bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lnc‐Pim1 Promotes Neurite Outgrowth and Regeneration of Neuron‐Like Cells Following ACR‐Induced Neuronal Injury
Authors: Li He , Jiang Ruo yu , Tang Ya jie , Ling Cong , Liu Fang , Xu Jia jun 
Published: October 2024

ABSTRACTDecreased regenerative capacity of central nervous system neurons is the main cause for failure of damaged neuron regeneration and functional recovery. Long noncoding RNAs (lncRNAs) are abundant in mammalian transcriptomes, and many time‐ and tissue‐specific lncRNAs are thought to be closely related to specific biological functions. The promoting effect of Pim‐1 gene on neural differentiation and regeneration has been documented, but the effect and mechanism of its neighbor gene Lnc‐Pim1 in regulating the response of central neurons to injury remain unclear. RT‐PCR in this study demonstrated that the expression of Lnc‐Pim1 was upregulated in acrylamide (ACR)‐induced neuronal injury. FISH and nucleus‐cytoplasmic assay demonstrated that Lnc‐Pim1 was mainly expressed in the neuron cytoplasm, with a small amount in the nucleus. Western blot analysis proved that Lnc‐Pim1 overexpression induced by the lentivirus vector could promote neurite outgrowth in Neuro‐2a cells by activating the Erk1/2 signal pathway, and improve neurite regeneration of injured neurons by upregulating GAP‐43 and β‐Ⅲ tubulin protein expression. However, silencing Lnc‐Pim1 expression by interfering RNA could effectively downregulate the GAP‐43 and β‐Ⅲ tubulin protein expression, and inhibit neurite growth of neurons. In addition, CHIRP‐MS was performed to identify several potential targets of Lnc‐Pim1 involved in the regulation of neurite regeneration of injured neurons. In conclusion, our study demonstrated that Lnc‐Pim1 is a potential lnc‐RNA, playing an important role in regulating central nerve regeneration.
Examining dimensions of help‐seeking intentions and associations with personal mental illness stigma among racial and ethnic minority immigrants
Authors: Deng Yali , Rose Theda 
Published: October 2024

AbstractThis study examined the factor structure of the General Help‐seeking Questionnaire and how personal mental illness stigma was associated with different types of help‐seeking intentions among racial and ethnic minority immigrants in the United States. A sample of 202 immigrants aged 18−39 were recruited from a Qualtrics panel and completed the online survey. Confirmatory factor analysis and multiple linear regression were conducted. Results confirmed four dimensions of help‐seeking intentions: informal, formal, semi‐formal, and traditional sources. Immigrants with higher levels of mental illness stigma are more likely to seek help from traditional sources (e.g., religious leader or other community‐based nonfaith resources) and semi‐formal sources (e.g., web‐based resources). Lack of awareness of service options is a key barrier to formal help‐seeking. Results signal the importance of collaboration between mental health service providers. Adopting culturally sensitive care and web‐based delivery formats may help address immigrants' concerns about mental health stigma.
Ethionine‐induced S‐adenosylmethionine deficiency suppressed H3K27me3 and cell differentiation during neural tube development in mice
Authors: Zhang Li , Zhang Xiaona , Liu Yurong , Wei Kaixin , Ma Huijing , Xia Li , Cao Rui , Sun Yuqing , Zheng Ronghua , Wang Xiuwei , Chang Bingmei 
Published: October 2024

AbstractS‐adenosylmethionine (SAM) as a major methyl donor plays a key role in methylation modification in vivo, and its disorder was closely related to neural tube defects (NTDs). However, the exact mechanism between SAM deficiency and NTDs remained unclearly. Hence, we investigated the association between histone methylation modification and cell differentiation in NTDs mice induced by SAM deficiency. The levels of SAM and SAH (S‐adenosylhomocysteine) were determined by enzyme linked immunosorbent assay (ELISA). The level of histone methylation, β‐catenin were analyzed by Western blot, reversing transcription and quantitative PCR (RT‐qPCR) and immunofluorescence. The results showed that the incidence rate of NTDs induced by ethionine were 46.2%. Post treatment of ethionine combined with SAM, the incidence rate of NTDs was reduced to 26.2%. The level of SAM was significantly decreased (p < 0.05) and a reduction in the SAM/SAH ratio was observed after entionine treatment. The SAM deficiency caused the reduction of H3K27me3 modifications and the elevated UTX activity (p < 0.05), and inhibited the expressions of β‐catenin. The differentiations of NSCs into neurons and oligodendrocytes were inhibited under SAM deficiency (p < 0.05). These results indicated that the SAM deficiency led to reduce H3K27me3 modifications, prevented the β‐catenin signaling pathway and NSCs differentiation, which provided an understanding of the novel function of epigenetic regulation in NTDs.
Fetal Diagnosis of a Ductus Arteriosus Aneurysm: A Case Report
Authors: Molina‐Giraldo Saulo , Galindez‐Guerrero Christian Camilo , Estupiñan‐Rincon Wilber , Sterling‐Castaño Diana , Acuña‐Osorio Edgar Mariano , Salazar Gustavo , Rojas‐Arias José Luis 
Published: October 2024

ABSTRACTThe ductus arteriosus aneurysm (DAA) is considered a rare anatomical alteration that consists of a dilation of this vascular structure. It has been reported that the DAA can resolve in the immediate postnatal stage and do not generate any consequences for the neonate. However, have been described some cases in which the DAA is complicated due to thromboembolic events, rupture of the lesion, respiratory symptoms, and even death. We present a case report of aneurysm of the ductus arteriosus diagnosed at 24 weeks of gestation with detailed imaging study. Also, we highlight the importance of the use of fundamental tools in the diagnosis: 3D ultrasound, multiplanar reconstruction, spatio‐temporal image correlation (STIC), and omniview.
Efficacy of Administration Routes in Crayfish: Comparative Analysis of Intracoelomic and Intrapericardial Techniques Using Fluorescein Dye
Authors: Kaur Davinder , Khan Areeba , Kubec Jan , Breithaupt Thomas , Buřič Miloš 
Published: October 2024

ABSTRACTCrayfish are emerging as model organisms for various disciplines. Moreover, decapod crustaceans also exhibit pain‐like reactions and heightened anxiety when exposed to harmful stimuli, leading to short‐term or persistent behavioral shifts. Awareness of decapod crustacean sentience and thus, suffering calls for refinement of current laboratory protocols. This study aims to enhance the standard methodology for injecting substances into crayfish by minimizing stress‐inducing manipulation. We examined the impacts of various administration routes on the persistence of injected chemicals in marbled crayfish, its excretion, and animal survival. Fluorescein dye was used as a visual marker. It was administered via three alternative injection routes—intracoelomic (IC), intrapericardial administration through areola (IP‐A), and intrapericardial administration through arthrodial membrane (IP‐AM). Continuous video observations were made for a 4‐h period under UV light, followed by intermittent observations at 12‐h intervals over 48 h. The highest mortality (20%) was observed in IP‐A administration. The IP‐A method also provided the fastest systemic distribution of the dye in the body. Results indicated visibly higher urination frequency in IP‐AM compared to IP‐A. IC mirrored IP‐AM outcomes without any observed mortality. We conclude that IC administration proved superior to intrapericardial methods, offering the least harmful but effective approach for crayfish injections.
The complexity of the perfect matching‐cut problem
Authors: Bouquet Valentin , Picouleau Christophe 
Published: October 2024

AbstractPERFECT MATCHING‐CUT is the problem of deciding whether a graph has a perfect matching that contains an edge‐cut. We show that this problem is NP‐complete for planar graphs with maximum degree four, for planar graphs with girth five, for bipartite five‐regular graphs, for graphs of diameter three, and for bipartite graphs of diameter four. We show that there exist polynomial‐time algorithms for the following classes of graphs: claw‐free, ‐free, diameter two, bipartite with diameter three, and graphs with bounded treewidth.
Inhibiting KSHV replication by targeting the essential activities of KSHV processivity protein, PF‐8
Authors: Travis Jennifer Kneas , Costantini Lindsey M. 
Published: October 2024

AbstractKaposi's Sarcoma Herpesvirus (KSHV) is the causative agent of several human diseases. There are no cures for KSHV infection. KSHV establishes biphasic lifelong infections. During the lytic phase, new genomes are replicated by seven viral DNA replication proteins. The processivity factor's (PF‐8) functions to tether DNA polymerase to DNA, so new viral genomes are efficiently synthesized. PF‐8 self‐associates, interacts with KSHV DNA replication proteins and the viral DNA. Inhibition of viral DNA replication would diminish the infection within a host and reduce transmission to new individuals. In this review we summarize PF‐8 molecular and structural studies, detail the essential protein‐protein and nucleic acid interactions needed for efficient lytic DNA replication, identify future areas for investigation and propose PF‐8 as a promising antiviral target. Additionally, we discuss similarities that the processivity factor from Epstein‐Barr virus shares with PF‐8, which could promote a pan‐herpesvirus antiviral therapeutic targeting strategy.
Maximum Bandwidth Analysis With a UniversalAWLR‐Based Filter Structure
Authors: Tang Xianli , Jia Yonghao , Yang Taojun , Hu Junyuan , Lu Wei‐Bing 
Published: October 2024

ABSTRACTThe bandwidth enhancement of the acoustic‐wave‐lumped‐element resonator (AWLR)‐based filter has been investigated for a long time. However, few researches are focused on the maximum bandwidth analysis. Based on a universal circuit structure of the AWLR‐based filter, we proposed the maximum bandwidth analysis of the hybrid filter. In the analysis process, the universal filter structure can be transformed into a novel AWLR‐based filter structure by incidentally excluding most of the other structures (including the reported hybrid filter structures) under the defined electrical characteristics. The novel hybrid filter obtains the maximum fractional bandwidth (FBW). Moreover, the proposed calculation results are better than the simulation results without artificial intervention. They are 2.12kt2and 1.63kt2, respectively.kt2is the electromechanical coupling coefficient of the acoustic wave resonator (AWR). Third‐order AWLR‐based bandpass filters with five‐type circuit structures have been manufactured and measured. They include the proposed novel AWLR‐based filter and the hybrid filter with the reported structure based on the universal AWLR‐based filter structure. The experimental results indicate that the proposed novel AWLR‐based filter has the maximum FBW.
Structure–property relationship of pea protein microgels as fat analogues in Pickering oil‐in‐water emulsions: effect of salt addition
Authors: Du Liyang , Li Shaoyang , Lan Yaqi , Meng Zong 
Published: October 2024

AbstractBACKGROUNDThe design of plant‐based microgels provides a platform for food ingredients to enhance palatability and functionality. This work aimed to explore the modifying effect of salt addition (KCl) on the structure of pea protein microgel particles (PPI MPs), on the interfacial adsorption and characteristics of formed emulsions as fat analogues.RESULTSSalt addition (0–200 mmol L−1) promoted a structural transformation from α‐helix to β‐sheet, increased the surface hydrophobicity (from 1160.8 to 2280.7), and increased the contact angle (from 56.73° to 96.47°) of PPI MPs. The electrostatic shielding effect led to the tighter packing of MPs with irregular structures and lowered the adsorption energy barrier. Notably, salt‐treated PPI MPs could adjust their adsorption state at the interface. The discernible adsorption of PPI MPs with 200 mmol L−1 salt addition that possessed enhanced anti‐deformation ability dominated the interfacial stabilization, whereas a relatively rougher stretched continuous interfacial film formed after spreading and deformation of 0 mmol L−1 MPs. A tribological test suggested that emulsion stabilized by MPs at 0 (0.0053) and 80 mmol L−1 (0.0068) had similar friction coefficients to commercial mayonnaise (0.0058), whereas a higher salt concentration (200 mmol L−1) lowered its oral sensation due to the adsorption layer and enhanced the resistance to droplet coalescence during oral processing.CONCLUSIONSalt could be a modifier to tune the structure of microgels, and further promote the formation and attributes of emulsions. This study would improve application attributes of PPI MPs in the design of realistic fat analogues. © 2024 Society of Chemical Industry.
Thermal stability of γ‐PGA modified fish gelatin emulsion
Authors: Xie Huan , Sha Xiao‐Mei , Shan Shan , Hu Zi‐Zi , Tu Zong‐Cai 
Published: October 2024

AbstractBACKGROUNDEmulsions are thermally unstable systems. This research aimed to investigate the thermal stability of fish gelatin (FG) oil‐in‐water emulsions in the presence of poly‐γ‐glutamic acid (γ‐PGA) as an additive after heat treatment. The study assessed how γ‐PGA influences the thermal stability of FG emulsions over time, focusing on their properties, structure, and food application potential.RESULTSThe incorporation of γ‐PGA significantly enhanced the thermal stability of FG emulsions, preserving their morphology after heating. Emulsions containing 0.1% γ‐PGA showed no significant changes after 24 h at 90 °C, while emulsions without γ‐PGA experienced noticeable delamination. Rheological evaluations revealed that the energy storage modulus and loss modulus of FG–γ‐PGA emulsions remained consistently higher than those of FG emulsions, regardless of heating duration. Particle size analysis indicated minimal changes for FG–γ‐PGA emulsions (413 nm after 24 h) compared to a substantial increase for FG emulsions (1598 nm). After heating, FG–γ‐PGA emulsions demonstrated significantly higher emulsifying activity index (EAI) (74 m2 g−1 versus 22.7 m2 g−1) and emulsifying stability index (ESI) (97% versus 76%). Additionally, the texture properties of meat mince formulated with FG‐γ‐PGA emulsions were comparable to those containing fat, showcasing their potential as a fat replacement.CONCLUSIONThe study concludes that γ‐PGA enhances the thermal stability of FG emulsions, maintaining their integrity and improving functional properties under heat treatment. These findings offer valuable insights for the formulation of thermally stable emulsions, presenting promising opportunities for innovative applications in the food industry. © 2024 Society of Chemical Industry.
Beyond Convenience: Exploring the Hidden Impact of Algorithmic Pricing on Consumer Trust and Loyalty in Chinese Online Travel Agencies
Authors: Wang Kuo‐Yan , Yu Jing , Chen Wei , Chen Jinchao 
Published: October 2024

ABSTRACTThe emergence of online travel agencies (OTAs) provides travelers with “one‐stop” convenience, but also faces issues of price discrimination. This mixed‐methods study began with semi‐structured interviews of 30 participants to explore initial perceptions, followed by a survey of 307 Chinese consumers. The study examined attitudes toward OTA overreliance on algorithmic pricing and unclear information, and their impact on trust and loyalty. Findings reveal that during peak seasons, regular customers without value‐added membership services paid higher room rates than new customers. The results indicate that once trust is established through clear information quality and reliable service, consumers are less wary of pricing quotes. The conclusion summarizes the findings and suggests further research to promote transparent and fair pricing practices, enhancing consumer trust and loyalty.
Understanding of Nationwide Camping Behavior in Korea: The Influence of Camping Regions and Type of Accommodation on the Costs Incurred
Authors: Kim Hak‐Jun , Kim Joonhyeong Joseph , Yoon Tae‐Hwan 
Published: October 2024

ABSTRACTThis study aims to investigate the main effect, and interaction effect of, camping regions and type of accommodation (i.e., general tent; glamping; camping car, caravan, trailer; car including general/modified vehicle) on campers' spending in terms of total, transportation, and accommodation costs. This study recruited a total of 3087 respondents based on the online panel who experienced camping between January 1, 2021 and December 31, 2021, in order to conduct two‐way ANOVA. Based on the analyses, the current study found a significant effect of the type of accommodation and camping regions on total, transportation, and accommodation costs. The significant interaction effect of camping regions and total, transportation, and accommodation costs suggests that a comprehensive understanding of campers' different needs for the type of accommodation in a diverse range of regions, specifically in Korea, is needed. As a result of the study, the practical implications for the operators of campsites were provided.
Insight into Structure, Regulatable Photoluminescence of Stably Self and Codoped Phosphor Ba3Y2B6O15: Dy/Eu for nUV wLEDs
Authors: Wang Chang , Yue Li , Li Wanyuan , Lei Yifeng , Xu Man , Dai Wubin 
Published: October 2024

AbstractThe exploitation of borate‐based phosphors is garnered tremendous attention in wLEDs. Although previous research provided some insights into borates from the viewpoints of synthesis, structure, and defect evolutions, their photoluminescence (PL) with dopants is still insufficient for actual application, especially on aspects of efficiency/stability. Herein, to alleviate the above issues, a new type of lesser‐known high symmetry Ba3Y2B6O15 (BYB) codoped with Dy3+/Eu3+ phosphors are synthesized via multistep solid‐state reaction and possessed controllable PL based on nUV excitation, defect‐induced (self‐) PL, and energy transfer (ET) from Dy3+ to Eu3+. The origin of blue self‐PL is clarified based on oxygen‐related defects and optimized via adjusting synthetic atmosphere (air/Ar) and (co)doping dopants to control the defect content. Thanks to dense connectivity, and highly symmetric structure with perfectly ordered octahedral sites of Y3+ for accommodation of Dy/Eu and ET, “cool” white and red PL from Dy3+ and Eu3+, respectively, show high PL efficiency with narrow full width at half the maximum (fwhm). The phosphor also exhibits excellent thermal stability because of high bandgap and low electron‐phonon coupling. Finally, a high‐quality phosphor‐converted wLED (pc‐wLED) device is assembled via remote “capping” packaging by adopting the BYB: Dy/Eu and a 350 nm nUV LED chip.
Simultaneous Probing of Electron Density and Temperature Dynamics Inside Air Plasma with Intense Terahertz Pulses
Authors: Zhang Minghao , Wang Guoyang , Zhang Yizhuo , Xiao Wen , Zhang Cunlin , Liu Yi , Tikhonchuk Vladimir , Zhang Liangliang 
Published: October 2024

AbstractAir plasma induced by ultrafast laser pulses is an extraordinary source of electromagnetic waves, emitting microwave, terahertz (THz) radiation, and cavityless lasing in the near‐infrared and visible ranges. The temporal dynamics of the electron density have been revealed by optical pump‐probe techniques, while the evolution of the electron temperature remains elusive due to a lack of suitable methods. Here, it is demonstrated that the intense THz‐field‐enhanced fluorescence emission from the excited molecules of nitrogen is a novel tool that allows to explore the complex dynamics of the plasma density and electron temperature simultaneously. Two relaxation times of electrons in air plasma are observed and interpreted as a competition between the excitation of a triplet state by laser or THz‐field‐heated electrons and the dissociative recombination of nitrogen molecular ions. Based on the theoretical simulations, the tens of picoseconds relaxation process is attributed to the ultrafast temperature decrease, while the longer relaxation in the range of hundreds of picoseconds is ascribed to the decay of electron density. The temporal relaxation of both the electron density and temperature revealed by applying an intense THz field provides further insights into the laser‐air plasma interaction and will benefit the engineering of this exceptional source.
Understanding the Effect of Corrosion Resistance in Welded AA2198‐T8 Alloy: Microstructural‐Electrochemical Insights
Authors: Machado Caruline de Souza C. , Araujo João V. S. , Hernández José W. C. , Milagre Mariana X. , Ramirez Oscar M. P. , de Melo Hercílio G. , Farooq Sajid , Costa Isolda 
Published: October 2024

ABSTRACTIn this study, we explore the corrosion resistance of AA2198‐T8 alloy in friction stir‐welded zones, focusing on microstructure changes and resulting corrosion susceptibility. Macrogalvanic coupling effects are observed in weldment under corrosive conditions, particularly in the welding joint (WJ), heat‐affected zones, and base metal (BM). Severe localized corrosion is prevalent in anodic zones, revealing intricate trenching patterns around micrometric particles in cathodic domains. Investigating isolated zones exposes SLC across all welding‐affected regions and BM, underscoring the involvement of microgalvanic cells in corrosion. The novel utilization of a syringe cell as an electrochemical tool proves to be instrumental in delineating the electrochemical characteristics of welding testing zones. Notably, stir zone within WJ emerges as the region depicting the lowest corrosion resistance, a critical finding substantiated by localized electrochemical impedance spectroscopy. These insights into the interplay of electrochemical phenomena and microstructural attributes have implications for advancing corrosion mitigation strategies and alloy engineering in materials science.
Empathy at the heart of customer experience: A holistic framework for understanding and enhancing consumer empathy through the lens of customer experience
Authors: Lehnert Leon , Kuehnl Christina 
Published: October 2024

AbstractServing as a social glue in interactions, consumer empathy takes center stage in shaping a pleasant customer experience. Our holistic framework of an empathic customer experience, rooted in the perception–action model of empathy and established customer experience conceptualizations, unveils the close interrelationship between consumer empathy and customer experiences. Grounded in a systematic literature review and a conceptual synthesis, our study contributes to customer experience research by conceptualizing consumer empathy as a cognitive and affective customer experience response, potentially accompanied by supplementary customer experience responses, which we refer to as an empathic customer experience. This study advances empathy literature by offering a systematic overview of an empathic customer experience, covering stimuli at touchpoints that induce an empathic customer experience during consumer interactions, potential contextual factors, and evaluative outcomes of an empathic customer experience. From our results, we derive an agenda with several research directions for the joint investigation of consumer empathy and customer experience to contribute to both research fields. In addition, our framework provides insights for managers, including recommendations for designing stimuli to evoke an empathic customer experience.