bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurements of Oleic Acid among Individual Kernels Harvested from Test Plots of Purified Runner and Spanish High Oleic Seed
Authors: Davis J. P. , Leek J.M. , Sweigart D.S. , Dang P. , Butts C.L. , Sorensen R.B. , Chen C.Y. , Lamb M.C. 
Published: August 2017

ABSTRACT
Normal oleic peanuts are often found within commercial lots of high oleic peanuts when sampling among individual kernels. Kernels not meeting high oleic threshold could be true contamination with normal oleic peanuts introduced via poor handling, or kernels not meeting threshold could be immature and not fully expressing the trait. Beyond unintentional mixing, factors contributing to variation in oleic acid concentration in peanut kernels include market type, environment, maturity and/or kernel size; however, the relative influence of these factors, and their interactions, is not quantitatively well understood on the single kernel level. To better understand these factors while simultaneously excluding variation from unintentional mixing, seed from a high oleic spanish cultivar and seed from a high oleic runner cultivar were carefully purified via NIR technology. The purified seed were planted in environmentally controlled test plots to analyze the progeny for oleic acid chemistry. Post flowering, plot sections were either chilled (3.8 -5.0 C below ambient), maintained at ambient or heated (3.8-5.0 C above ambient) in the pod zone to characterize soil temperature effects on oleic acid chemistry development. Fully randomized (4 reps) plots included the purified high oleic spanish and runner cultivars, three soil temperatures, seed maturity (profile board), commercial kernel size classifications, and a late season flower termination protocol. At harvest, the oleic acid concentration of approximately 24,000 individual kernels were measured via NIR technology. Market type, temperature, maturity and size had a significant effect on high oleic chemistry among kernels. Late season flower termination significantly, and positively, influenced high oleic chemistry of runner peanuts, minimized the number of immature kernels not meeting high oleic threshold and resulted in elevated and more consistent distributions in this key chemistry; distributions that were more similar to those of the more botanically determinate, but lower yielding, spanish market type. Data from this study improves our understanding of expected natural variation in high oleic chemistry and suggests late season flower termination of runner peanuts is a viable strategy to maximize high oleic chemistry on the single kernel level.
Longitudinal changes in knee muscles isokinetic strength and dynamic performance in patients following reconstruction of the anterior cruciate ligament
Authors: Seo Kyoung Ho , Lee Joong Hoon , Lee Seung-Yeol , Chang Chong-Bum , Lim Jae-Young 
Published: August 2017

BACKGROUND: Relatively little is known about the longitudinal changes in strength and function in the early phase compared to long term outcome following reconstruction of the anterior cruciate ligament (ACL). OBJECTIVE: To assess the isokinetic concentric strength of the knee extensors and flexors as well as the distance jumped in the one-legged hop test in patients before and at 3, 6 and 12 months after (6 and 12 months for the hop test) reconstruction of the ACL. METHOD: Seventy-three men, aged 18–50 years took part in the study. The primary outcome measures were the International Knee Documentation Committee (IKDC) scores at 3, 6, and 12 months after surgery and one-legged hop test at 6 and 12 months after post-reconstruction. RESULTS: There was a consistent increase in muscular strength between 3 and 12 months post-reconstruction. Fair but significant correlations were found between the IKDC score at 12 months and the peak moment of the knee extensors at 3 and 6 months ([Formula: see text] 0.450 and 0.429, respectively, [Formula: see text] 0.012). 60.2% of the participants satisfied the criteria of successful outcome based on IKDC scores and one-legged hop test at 12 months. The peak moment of knee extensor measured at 3 months after surgery was a significant predictive factor for successful outcome ([Formula: see text] 0.021). Patients in whom muscle strength had recovered well by 3 months after ACL reconstruction were more active in terms of sporting activities and exhibited better dynamic performance at the 1-year follow-up. CONCLUSION: Knee extension strengthening in early phase is crucial to restoring sporting performance after ACL reconstruction.
Culture-Positive Endophthalmitis After Open Globe Injuries With and Without Retained Intraocular Foreign Bodies
Authors: Banker Tanuj P. , McClellan Andrew J. , Wilson Benjamin D. , Juan Fabiana M. , Kuriyan Ajay E. , Relhan Nidhi , Chen Felix V. , Weichel Eric D. , Albini Thomas A. , Berrocal Audina M. , Sridhar Jay , Gregori Ninel Z. , Townsend Justin H. , Flynn Harry W. 
Published: August 2017

BACKGROUND AND OBJECTIVE:
To report the visual and anatomical outcomes and microbiologic spectrum of culture-positive endophthalmitis in open globe injuries (OGIs) with or without intraocular foreign bodies (IOFBs).

PATIENTS AND METHODS:
A retrospective, interventional case series of OGIs (n = 718) treated between 2004 and 2015. Patients underwent a management protocol for OGI, including systemic broad-spectrum antibiotics, on presentation.

RESULTS:

Culture-positive cases of endophthalmitis after open globe repair occurred in 2.1% of eyes (n = 15 of 718 eyes); two eyes had evidence of endophthalmitis on presentation. The most common organism was
Staphylococcus
species (five of 17 eyes). An IOFB was present in 6.8% of eyes (n = 49 of 718 eyes). All of these eyes received prophylactic intravitreal antimicrobials. In eyes with IOFB, the rate of culture-positive endophthalmitis after initial globe repair was 8.1% (n = 4 of 49 eyes) versus 1.6% (n = 11 of 669 eyes) in eyes without IOFB (
P
< .01).

CONCLUSION:
Culture-positive endophthalmitis was identified after OGIs more often in eyes with a concurrent IOFB.

[
Ophthalmic Surg Lasers Imaging Retina
. 2017;48:632–637.]
IRF4 Modulates CD8+ T Cell Sensitivity to IL-2 Family Cytokines
Authors: Huang Su , Shen Yingjia , Pham Duy , Jiang Li , Wang Zheng , Kaplan Mark H , Zhang Guangjun , Sun Jie 
Published: August 2017

Abstract
IFN regulatory factor 4 (IRF4) is a key transcription factor that promotes effector CD8+ T cell differentiation and expansion. The roles of IRF4 in regulating the CD8+ T cell response to cytokines have not been explored. In this article, we show that IL-2 and IL-15 signaling and STAT5 activation regulate IRF4 expression in CD8+ T cells. Gene-expression profile analysis has also revealed that IRF4 is required for expression of the receptors of IL-2 family cytokines CD122 and CD127. We found that IRF4 binds directly to CD122 and CD127 gene loci, indicating that it may directly promote CD122 and CD127 gene transcription. As a consequence, IRF4-deficient CD8+ T cells show diminished sensitivity to IL-2, IL-15, and IL-7 treatment in vitro. Furthermore, we found that IRF4-deficient CD8+ T cells had lower expression of CD122 and CD127 in vivo during influenza virus infection. These data suggest that IRF4 regulates the sensitivity of CD8+ T cells to IL-2 family cytokines, which correlates with the diminished effector and memory CD8+ T cell responses in IRF4-deficient CD8+ T cells.
A Novel Computational Model Predicts Key Regulators of Chemokine Gradient Formation in Lymph Nodes and Site-Specific Roles for CCL19 and ACKR4
Authors: Jafarnejad Mohammad , Zawieja David C. , Brook Bindi S. , Nibbs Robert J. B. , Moore James E. 
Published: August 2017

Abstract
The chemokine receptor CCR7 drives leukocyte migration into and within lymph nodes (LNs). It is activated by chemokines CCL19 and CCL21, which are scavenged by the atypical chemokine receptor ACKR4. CCR7-dependent navigation is determined by the distribution of extracellular CCL19 and CCL21, which form concentration gradients at specific microanatomical locations. The mechanisms underpinning the establishment and regulation of these gradients are poorly understood. In this article, we have incorporated multiple biochemical processes describing the CCL19–CCL21–CCR7–ACKR4 network into our model of LN fluid flow to establish a computational model to investigate intranodal chemokine gradients. Importantly, the model recapitulates CCL21 gradients observed experimentally in B cell follicles and interfollicular regions, building confidence in its ability to accurately predict intranodal chemokine distribution. Parameter variation analysis indicates that the directionality of these gradients is robust, but their magnitude is sensitive to these key parameters: chemokine production, diffusivity, matrix binding site availability, and CCR7 abundance. The model indicates that lymph flow shapes intranodal CCL21 gradients, and that CCL19 is functionally important at the boundary between B cell follicles and the T cell area. It also predicts that ACKR4 in LNs prevents CCL19/CCL21 accumulation in efferent lymph, but does not control intranodal gradients. Instead, it attributes the disrupted interfollicular CCL21 gradients observed in Ackr4-deficient LNs to ACKR4 loss upstream. Our novel approach has therefore generated new testable hypotheses and alternative interpretations of experimental data. Moreover, it acts as a framework to investigate gradients at other locations, including those that cannot be visualized experimentally or involve other chemokines.
Klhl6 Deficiency Impairs Transitional B Cell Survival and Differentiation
Authors: Bertocci Barbara , Lecoeuche Damiana , Sterlin Delphine , Kühn Julius , Gaillard Baptiste , De Smet Annie , Lembo Frederique , Bole-Feysot Christine , Cagnard Nicolas , Fadeev Tatiana , Dahan Auriel , Weill Jean-Claude , Reynaud Claude-Agnès 
Published: August 2017

Abstract
Klhl6 belongs to the KLHL gene family, which is composed of an N-terminal BTB-POZ domain and four to six Kelch motifs in tandem. Several of these proteins function as adaptors of the Cullin3 E3 ubiquitin ligase complex. In this article, we report that Klhl6 deficiency induces, as previously described, a 2-fold reduction in mature B cells. However, we find that this deficit is centered on the inability of transitional type 1 B cells to survive and to progress toward the transitional type 2 B cell stage, whereas cells that have passed this step generate normal germinal centers (GCs) upon a T-dependent immune challenge. Klhl6-deficient type 1 B cells showed a 2-fold overexpression of genes linked with cell proliferation, including most targets of the anaphase-promoting complex/cyclosome complex, a set of genes whose expression is precisely downmodulated upon culture of splenic transitional B cells in the presence of BAFF. These results thus suggest a delay in the differentiation process of Klhl6-deficient B cells between the immature and transitional stage. We further show, in the BL2 Burkitt’s lymphoma cell line, that KLHL6 interacts with Cullin3, but also that it binds to HBXIP/Lamtor5, a protein involved in cell-cycle regulation and cytokinesis. Finally, we report that KLHL6, which is recurrently mutated in B cell lymphomas, is an off-target of the normal somatic hypermutation process taking place in GC B cells in both mice and humans, thus leaving open whether, despite the lack of impact of Klhl6 deficiency on GC B cell expansion, mutants could contribute to the oncogenic process.
Regulation of the Cell Cycle and Inflammatory Arthritis by the Transcription Cofactor LBH Gene
Authors: Matsuda Shinji , Hammaker Deepa , Topolewski Katharyn , Briegel Karoline J. , Boyle David L. , Dowdy Steven , Wang Wei , Firestein Gary S. 
Published: August 2017

Abstract
Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) display unique aggressive behavior, invading the articular cartilage and promoting inflammation. Using an integrative analysis of RA risk alleles, the transcriptome and methylome in RA FLS, we recently identified the limb bud and heart development (LBH) gene as a key dysregulated gene in RA and other autoimmune diseases. Although some evidence suggests that LBH could modulate the cell cycle, the precise mechanism is unknown and its impact on inflammation in vivo has not been defined. Our cell cycle analysis studies show that LBH deficiency in FLS leads to S-phase arrest and failure to progress through the cell cycle. LBH-deficient FLS had increased DNA damage and reduced expression of the catalytic subunit of DNA polymerase α. Decreased DNA polymerase α was followed by checkpoint arrest due to phosphorylation of checkpoint kinase 1. Because DNA fragments can increase arthritis severity in preclinical models, we then explored the effect of LBH deficiency in the K/BxN serum transfer model. Lbh knockout exacerbated disease severity, which is associated with elevated levels of IL-1β and checkpoint kinase 1 phosphorylation. These studies indicate that LBH deficiency induces S-phase arrest that, in turn, exacerbates inflammation. Because LBH gene variants are associated with type I diabetes mellitus, systemic lupus erythematosus, RA, and celiac disease, these results suggest a general mechanism that could contribute to immune-mediated diseases.
A escrita sociológica e as temáticas dos livros coletâneas: debates contínuos e descontínuos
Authors: Eras Lígia Wilhelms 
Published: August 2017

http://dx.doi.org/10.5007/1980-3532.2015n14p12 O artigo apresenta o debate dos livros coletâneas sobre o Ensino de Sociologia na Educação Básica na identificação das temáticas localizadas nos livros coletâneas (2008-2013), em metodologias de pesquisa (bibliográfica temática e teórica, histórica, prosopográfica e de análise de conteúdos) predominando na composição do artigo, a análise de conteúdos. O que prevalece no cenário como um todo é um movimento de continuidades e descontinuidades quanto à disposição de incorporar uma maior circulação institucional das ideias das experiências de ensino situadas em eventos acadêmicos, projetos de fomento à investigação e iniciação à docência e a uma prévia superação do registro imediato das experiências. Porém, as continuidades ainda se revelam em produções com baixas tiragens e a uma lacuna no avanço de um maior dialogismo entre a produção dos capítulos, edições, inter-obras e as apostas nos inventos teórico-didáticos específicos da subárea.
БАГАНУУР, ТАВАНТОЛГОЙН НҮҮРСНИЙ ТЕРМОГРАФИ, ТЕРМОЛИЗИЙН СУДАЛГАА
Authors: Д Каминский Ю , Ж Дугаржав , Б Авид 
Published: August 2017

Нийлэг түлш, кокс химийн аж үйлдвэрийг үүсгэн хөгжүүлэх зорилт тавигдаж байгаа өнөө үед Багануур, Тавантолгой мэтийн томоохон ордуудын нүүрсний хими – бүтцийн төрх байдал, урвалын идэвх, ялангуяа, халуун задрал, хийгээд механохимийн урвалыг гүнзгий судлах нь ихээхэн ач холбогдолтой. Нөгөө талаар, багануур ба тавантолгойн нүүрс бол Монгол орны хүрэн ба коксждог чулуун нүүрсний түгээмэл төлөөлөгч тул бусад ордын ижил төсөөтөй нүүрсний төрх байдлын үнэлгээнд ч хөрвүүлэн ашиглах боломжтой. Энэ үүднээс бид ОХУ – ын ШУА – ийн Сибирийн салбар, Монголын ШУА- ийн хамтарсан төслийн хүрээнд хоёр ордын нүүрсний хими- бүтцийн гүнзгийрүүлсэн судалгаа явуулсан бөгөөд өмнөх өгүүлэлд /1/ хоёр ордын нүүрсний петрограф, морфологи болон химийн байгуулалтын судалгааны үр дүнгүүдийг гаргасан болно. Энэ өгүүлэлд хоёр ордын нүүрсний термографи, термолиз, механохимийн судалгааны дүнгүүдийг өгүүлэв.
Extraction and Determination of Trace Amounts of p-Coumaric Acid in Vinegar, Carrot Juice, and Seed Extract from Silybum marianum (L.) Gaertn
Authors: Khani Rouhollah , Rostami Zeinab , Bagherzade Ghodsieh , Khojeh Vahid 
Published: August 2017

Abstract
In this study, for the monitoring and quantification of p-coumaric acid (p-CA) in vinegar, carrot juice, and seed extract from the plant species Silybum marianum (L.) Gaertn, an efficient and low-cost analytical method has been applied. For this purpose, a dispersive liquid–liquid microextraction (DLLME) method, followed by UV-Vis spectrophotometric detection, was used. To form a cloudy solution, a binary mixture containing ethanol as a disperser solvent and chloroform as an extraction solvent was rapidly injected by syringe into a sample solution containing p-CA. After centrifugation, dilution of the obtained organic phase was done with the proper amount of ethanol, and the phase was transferred into a micro cell for subsequent measurement. Some effective parameters for the DLLME method, such as the volume of disperser solvent and extraction solvent, pH, and salt concentration were inspected by a 24 full factorial central composite design using design Export Software. Under the optimized conditions, linearity was between 10 and 150 ng/mL, and the LOD was 2.3 ng/mL. The results of the proposed method were similar to the obtained results using a GC with flame-ionization detection method.
Various Aspects of Bollard Pull Tests and Analysis of Test Results
Authors: Dev Arun Kr 
Published: August 2017

The static force exerted on a hawser at zero ship speed by a vessel, otherwise known as the bollard pull, is one of the key performance indicators of Tugs, Anchor Handling Tugs (AHTs) and Anchor Handling Tug Supply Vessels (AHTSVs). The value of bollard pull is considered critical as it defines the functionality and performance of the vessel. When a company decides on chartering a vessel, a definite prerequisite considered is the value of the bollard pull. The value may be obtained via three ways: calculations, model-testing, and full-scale trials. The latter is often used officially to certify the vessel's bollard pull rating, with the presence of the vessel's owners, surveyors, and any other third parties. The tests and trials follow a set of guidelines provided by classification societies but do not have a standardized set of rules. Therefore, disagreements often arise over the results of such tests and trials. Tests are often carried without any load cells measuring the shaft power to ascertain the brake horsepower (BHP). Simply, engine rpm/ rating is used to fix the 100% maximum continuous rating (MCR) which often in the range of 105–108% MCR. Some of the parties involved in certifying the correct bollard pull tests (BPTs) do not even understand what is all about the bollard pull. Everybody is looking for a higher figure for the bollard pull on the certificate when the reality is different. The author examines and discusses the broad spectrum of factors that affects the "true" value of the bollard pull and explains why such a standardized set of mandatory BPT and the trial code is deemed necessary. The author also presents some of the interesting BPTs data to show the differences in various ways of conducting BPTs.

1. Introduction
The bollard pull (BP) is the zero speed pulling/pushing capability of a vessel, i.e., Tugs, Anchor Handling Tug (AHTs), Anchor Handling Tug/ Towing Supply Vessel (AHTSVs), trawlers, etc. It is considered as one of the key practical performance indicators of the above-mentioned vessels, measuring the usefulness of the vessel in a stranding scenario or in holding large vessels such as tankers or towing a rig.
Analysis of budget changes in the municipality of Parnaíba Santana in the exercises of 2013 and 2014.
Authors: Scalabrin Cardoso Gomes Maria Helena , Ferreira Vanderlei , Pereira Rios Ricardo , Goulart Verci Douglas 
Published: August 2017

The aim of this study was to analyze the changes in the public budgets in Santana de Parnaíba, during the years of 2013 and 2014. The methodology used in this study started from the existing Laws, academic publications, followed by research on the electronic site. The analytical bases were those in the Transparency Index of Municipal Public Management (ITGP-M), followed by exploratory researches with the responsible for preparing the public budget. The study reveals that in the year 2014 were proposed 31 Decrees, which the amounting of R$ 129 million in additional credits. The same happened in the year 2013, R$119 million. The Municipal Secretaries of Education and Health received together 70.52% of the funds. The justifications given by the responsible officers regarding these supplementary credits were not compatible with acts of good management, since the changes were occurred in the early months of each year, characterizing, at least, an inadequate planning. The evidence of inadequate practices, generate questions and send warning signals that is an indicative of monitoring the municipal budget.
Occasional hybridization between a native and invasiveSeneciospecies in Australia is unlikely to contribute to invasive success
Authors: Dormontt Eleanor E. , Prentis Peter J. , Gardner Michael G. , Lowe Andrew J. 
Published: August 2017

BackgroundHybridization between native and invasive species can facilitate introgression of native genes that increase invasive potential by providing exotic species with pre-adapted genes suitable for new environments. In this study we assessed the outcome of hybridization between nativeSenecio pinnatifoliusvar.pinnatifoliusA.Rich. (dune ecotype) and invasiveSenecio madagascariensisPoir. to investigate the potential for introgression of adaptive genes to have facilitatedS. madagascariensisspread in Australia.MethodsWe used amplified fragment length polymorphisms (141 loci) and nuclear microsatellites (2 loci) to genotype a total of 118 adults and 223 seeds fromS. pinnatifoliusvar.pinnatifoliusandS. madagascariensisat one allopatric and two shared sites. We used model based clustering and assignment methods to establish whether hybrid seed set and mature hybrids occur in the field.ResultsWe detected no adult hybrids in any population. Low incidence of hybrid seed set was found at Lennox Head where the contact zone overlapped for 20 m (6% and 22% of total seeds sampled forS. pinnatifoliusvar.pinnatifoliusandS. madagascariensisrespectively). One hybrid seed was detected at Ballina where a gap of approximately 150 m was present between species (2% of total seeds sampled forS. madagascariensis).ConclusionsWe found no evidence of adult hybrid plants at two shared sites. Hybrid seed set from both species was identified at low levels. Based on these findings we conclude that introgression of adaptive genes fromS. pinnatifoliusvar.pinnatifoliusis unlikely to have facilitatedS. madagascariensisinvasions in Australia. Revisitation of one site after two years could find no remainingS. pinnatifoliusvar. pinnatifolius, suggesting that contact zones between these species are dynamic and thatS. pinnatifoliusvar. pinnatifoliusmay be at risk of displacement byS. madagascariensisin coastal areas.
Immunological responses and wound healing in the polychaete Eurythoe complanata (Annelida: Amphinomidae) exposed to copper
Authors: Zapata-Vívenes E , Nusetti OA , Marcano L , Esclapés MM , Arredondo L 
Published: August 2017

Polychaetes exposed to 0.4 mg Cu+2 L–1 (2.14 mg CuSO4 5H2O L–1; 30% 96 h-LC50) for seven days and control individuals were divided into three groups: the first two were sensitized and non-sensitized with Micrococcus lysodeikticus, and the third was sensitized with heat-killed yeast. Lysozyme activity was tested in the first two groups and phagocytosis in the third one. Lysozyme was measured at 2 h and phagocytosis 15 min after immune sensitization. The effect of copper on wound healing was also examined in the polychaetes. For this, the polychaetes exposed and not exposed to copper were cut in two halves, and the body fragments were held in aquaria containing clean seawater, and the cicatrization was periodically observed. The lysozyme and phagocytosis responses were induced by the sensitization; however, these responses were inhibited after the metal exposure. On the other hand, 60–80% of the body fragments from the control group healed within four days, whereas the copper-treated individuals did not show cicatrization in the same period of time. In conclusion, the inhibition of the experimental activation of lysozyme and phagocytosis, and of wound healing in the polychaete Eurythoe complanata exposed to acute copper exposure, suggests the metal´ s potential toxicity on the physiological mechanisms that modulate the innate immunity in annelids, whose alteration could affect their tolerance to microbial infection.