bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNHG14 promotes triple‐negative breast cancer cell proliferation, invasion, and chemoresistance by regulating the ERK/MAPK signaling pathway
Authors: Wang Bin , Xing Ai‐Yan , Li Guang‐Xin , Liu Long , Xing Chungen 
Published: September 2024

AbstractThe functional role and molecular mechanisms of small‐nucleolar RNA host gene 14 (SNHG14) in triple‐negative breast cancer (TNBC) progression remain unclear. The expression levels of SNHG14 in breast cancer samples and cell lines were determined using real‐time quantitative polymerase chain reaction. Cell proliferation, migration, and invasion abilities were detected using MTS and transwell assays. By RNA sequencing, differentially expressed genes were identified between the SNHG14 siRNA and the negative control group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to predict the targets and pathways regulated by SNHG14. pRAF, pMEK, and pERK expression were measured by western blot. The xenograft model was constructed to access the biological function of SNHG14 in vivo. A minimal patient‐derived xenograft model was established to evaluate the sensitivity to chemotherapy drugs. Our data indicated that SNHG14 expression was increased in TNBC tissues and cell lines. SNHG14 knockdown attenuated the proliferation, migration, and invasion abilities of TNBC cells both in vivo and in vitro. High SNHG14 expression was associated with lymph node metastasis and a high Ki67 index. The targets of SNHG14 were mainly enriched in the MAPK signaling pathway. pRAF, pMEK, and pERK expression were downregulated after being transfected with SNHG14 siRNA. Compared with the negative control group, the expression of CACNA1I, DUSP8, FGF17, FGFR4, FOS, PDGFRB, and DDIT3 was increased, and the expression of MKNK1 was decreased in the SNHG14 siRNA group. Minimal patient‐derived xenograft model demonstrated that knockdown of SNHG14 enhanced the sensitivity to Docetaxel in vivo. Compared with the DMSO group, the proliferation of Docetaxel‐resistant MDA‐MB‐231 cells was decreased in Dabrafenib, PD184352, and FR180204 treatment groups. SNHG14 knockdown inhibits TNBC progression by regulating the ERK/MAPK signaling pathway, which provides evidence for SNHG14 as a potential target for TNBC therapy.
Three‐Dimensionally Printed Biphasic Calcium Phosphate Ceramic Substrates as the Sole Inducer of Osteogenic Differentiation in Stromal Vascular Fraction Cells
Authors: Brochet Louis , Thomann Céline , Chocarro‐Wrona Carlos , Abawi Ariana , Nolens Grégory , Marquette Christophe , Dufour Alexandre 
Published: September 2024

ABSTRACTThe stromal vascular fraction (SVF) is a derivate of fat tissue comprising both adipose‐derived mesenchymal stem cells and endothelial cells and serves as a promising cell source for engineering vascularized bone tissues. Its combination with osteoconductive biphasic calcium phosphate (BCP) ceramic may represent a point‐of‐care agent for bone reconstruction. Here we assessed the proliferation and osteogenic differentiation capacities of SVF on 3D printed BCP implants, in comparison with isolated adipose‐derived mesenchymal stem cells (AD‐MSCs). AD‐MSCs and SVF isolated from human donors were seeded on plastic or 3D printed BCP ceramics with sinusoidal or gyroid macrotopography and cultured in the presence or absence of osteogenic factors. Vascular, hematopoietic and MSC surface markers were assessed by flow cytometry whereas osteogenic activity was investigated through alizarin red staining and alkaline phosphatase activity. Osteogenic factors were necessary to trigger osteogenic activity when cells were cultured on plastic, without significant difference observed between the two cell populations. Interestingly, osteogenic activity was observed on BCP implants in the absence of differentiation factors, without significant difference in level activity between the two cell populations and macrotopography. This study offers supportive data for the use of combined BCP scaffolds with SVF in a perspective of a one‐step surgical procedure for bone regeneration.
5‐Fluorouracil treatment represses pseudouridine‐containing miRNA export into extracellular vesicles
Authors: Qu Shimian , Nelson Hannah M. , Liu Xiao , Wang Yu , Semler Elizabeth M. , Michell Danielle L. , Massick Clark , Franklin Jeffrey L. , Karijolich John , Weaver Alissa M. , Coffey Robert J. , Liu Qi , Vickers Kasey C. , Patton James G. 
Published: September 2024

Abstract5‐Fluorouracil (5‐FU) has been used for chemotherapy for colorectal and other cancers for over 50 years. The prevailing view of its mechanism of action is inhibition of thymidine synthase leading to defects in DNA replication and repair. However, 5‐FU is also incorporated into RNA causing defects in RNA metabolism, inhibition of pseudouridine modification, and altered ribosome function. We examined the impact of 5‐FU on post‐transcriptional small RNA modifications (PTxMs) and the expression and export of RNA into small extracellular vesicles (sEVs). EVs are secreted by all cells and contain a variety of proteins and RNAs that can function in cell‐cell communication. We found that treatment of colorectal cancer (CRC) cells with 5‐FU represses sEV export of miRNA and snRNA‐derived RNAs, but promotes export of snoRNA‐derived RNAs. Strikingly, 5‐FU treatment significantly decreased the levels of pseudouridine on both cellular and sEV small RNA profiles. In contrast, 5‐FU exposure led to increased levels of cellular small RNAs containing a variety of methyl‐modified bases. These unexpected findings show that 5‐FU exposure leads to altered RNA expression, base modification, and aberrant trafficking and localization of small RNAs.
The genetic analysis of eight families with hemophilia B in Mongolia: Identification of two novel mutation
Authors: Munkhuu Purevdorj , Bazarragchaa Munkhtsetseg , Ichinkhorloo Purevdorj , Yoo Ki‐Young , Ayush Enkh‐Amar , Batjargal Ochbadrakh , Namjil Erdenebayar , Jav Sarantuya , Purevdorj Erkhembulgan , Lkhagvasuren Sodnomtsogt 
Published: September 2024

AbstractBackgroundThis study aimed to conduct molecular diagnostics among individuals with hemophilia B (HB) and carriers of hemophilia in Mongolia.MethodsEight patients (six severe, two mild) with HB and their 12 female relatives were enrolled from eight families. Sanger sequence was performed for mutation identification. The questionnaire survey was conducted to evaluate carrier symptoms in female relatives.ResultsTwo families had a history of HB. A total of five different variants (c.223C > T; c.344A > G; c.464G > C; c.187_188del; and c.1314_1314delA) were identified in six patients with severe HB. Of these, two (c.187_188del and c.1314_1314delA) were novel. No variant in the entire F9 was found in two patients with mild HB. Nonsense c.223C > T (p.Arg75*) mutation was detected in two unrelated patients. Carrier testing identified five mothers as carriers, while one younger sister was a non‐carrier. The carrier status of six female relatives of the two mild patients remained undetermined. By questionnaire survey, only one of the five genetically identified carriers displayed noticeable symptoms of being a carrier.ConclusionThe novel variants c.187_188del and c.1314_1314delA can cause severe hemophilia B. This study did not observe a significant association between symptoms and carrier status in the five carriers.
Development and external validation of a novel modality for rapid recognition of aortic dissection based on peripheral pulse oximetry waveforms
Authors: Luo Jing‐chao , Zhang Yi‐jie , Niu Ying , Luo Ming‐hao , Sun Feng , Tu Guo‐wei , Chen Zhao , Zhou Si‐ying , Gu Guo‐rong , Cheng Xu‐feng , Zhao Yu‐wei , Zhou Wan‐ting , Luo Zhe 
Published: September 2024

AbstractBackgroundAortic dissection (AD) is a life‐threatening cardiovascular emergency that is often misdiagnosed as other chest pain conditions. Physiologically, AD may cause abnormalities in peripheral blood flow, which can be detected using pulse oximetry waveforms.PurposeThis study aimed to assess the feasibility of identifying AD based on pulse oximetry waveforms and to highlight the key waveform features that play a crucial role in this diagnostic method.MethodsThis prospective study employed high‐risk chest pain cohorts from two emergency departments. The initial cohort was enriched with AD patients (n = 258, 47% AD) for model development, while the second cohort consisted of chest pain patients awaiting angiography (n = 71, 25% AD) and was used for external validation. Pulse oximetry waveforms from the four extremities were collected for each patient. After data preprocessing, a recognition model based on the random forest algorithm was trained using patients' gender, age, and waveform difference features extracted from the pulse oximetry waveforms. The performance of the model was evaluated using receiver operating characteristic (ROC) curve analysis and decision curve analysis (DCA). The importance of features was also assessed using Shapley Value and Gini importance.ResultsThe model demonstrated strong performance in identifying AD in both the training and external validation sets. In the training set, the model achieved an area under the ROC curve of 0.979 (95% CI: 0.961–0.990), sensitivity of 0.918 (95% CI: 0.873–0.955), specificity of 0.949 (95% CI: 0.912–0.985), and accuracy of 0.933 (95% CI: 0.904–0.959). In the external validation set, the model attained an area under the ROC curve of 0.855 (95% CI: 0.720–0.965), sensitivity of 0.889 (95% CI: 0.722–1.000), specificity of 0.698 (95% CI: 0.566–0.812), and accuracy of 0.794 (95% CI: 0.672–0.878). Decision curve analysis (DCA) further showed that the model provided a substantial net benefit for identifying AD. The median mean and median variance of the four limbs' signals were the most influential features in the recognition model.ConclusionsThis study demonstrated the feasibility and strong performance of identifying AD based on peripheral pulse oximetry waveforms in high‐risk chest pain populations in the emergency setting. The findings also provided valuable insights for future human fluid dynamics simulations to elucidate the impact of AD on blood flow in greater detail.
A new nomogram for the evaluation of detrusor underactivity and bladder outlet obstruction in nonneurogenic female patients with lower urinary tract symptoms who undergo urodynamic studies
Authors: Barco‐Castillo Catalina , Sotelo Perilla Melida , Rangel Amaya Jaime , Castaño Juan Carlos 
Published: September 2024

AbstractIntroductionMicturition physiology differs in men and women. However, the results in standard urodynamic studies in women with lower urinary tract symptoms (LUTS) were extrapolated from studies in men. Nowadays, the only validated nomogram for females is Solomon–Greenwell's. However, it only evaluated bladder outlet obstruction (BOO) without considering detrusor underactivity (DU). This study aims to create a nomogram that includes an evaluation of DU and BOO in nonneurogenic women and validate it against videourodynamic studies along with other nomograms.Materials and MethodsFor the first analysis (creation cohort), we included 183 women with LUTS who underwent videourodynamic study between 2022 and 2023. Exclusion criteria were females with neurologic diseases, renal transplantation, and trouble performing the flow‐pressure study. Baseline characteristics of the patients, urodynamic parameters, and classifications on different nomograms and indexes were evaluated. A logistic regression found Qmax and PdetQmax as predictors for DU and BOO. The Barco–Castillo nomogram was created by clustering analysis and adjusted by the results of the logistic regression. A second (test) cohort was evaluated from 2023 to 2024, including 142 patients for the validation of the nomogram. A p < 0.05 was considered significant.ResultsAll urodynamic parameters were compared between both cohorts, with no significant differences. The median age of the creation cohort was 50 years old (interquartile range [IQR] 39–63). All patients had LUTS and a previous standard urodynamic study without a clear diagnosis. The cluster analysis had a p < 0.05 for two groups of BOO (yes/no) and two of DU (yes/no). We created the graph based on the logistic regression results and adjusted it according to the data. The median age of the test cohort was 44 years old (IQR 33.75–59) and had the same indication for the videourodynamic study. The receiver operating characteristic (ROC) curve for BOO showed an accuracy of 85.4% for Barco–Castillo nomogram, 68.5% for Blaivas–Groutz, 58.1% for Solomon–Greenwell, 57.1% for BOOI, and 50% for LinPURR. For DU, accuracy was 80.5% for PIP‐1, 80.2% for Barco–Castillo, 76.6% for BCI, and 70.1% for LinPURR.ConclusionsWhen evaluating women's urodynamic studies, it is important to focus on female physiology and discourage the use of parameters previously standardized in men. We encourage using the new Barco–Castillo nomogram to determine BOO and DU in women as the currently easiest and more accurate tool.
Assessment of cognitive–motor functions in adults with perceived neuropsychological problems using NIH toolbox after remote biofield energy treatment as non‐pharmacological intervention: A randomized double‐blind placebo controlled trial
Authors: Trivedi Mahendra Kumar , Branton Alice , Trivedi Dahryn , Mondal Sambhu , Jana Snehasis 
Published: September 2024

AbstractNon‐pharmacological interventions include physical activity, biofield energy therapy, reiki, Tai chi, and therapeutic touch. However, no reports analyzed the effectiveness of biofield therapy on cognition and motor function performance in adult subjects. The study aimed to investigate the impact of remote biofield energy healing therapy on cognition and motor functioning in adults with self‐perceived neuropsychological impairments. This was a randomized double‐blind clinical trial that involved 114 participants with self‐perceived neuropsychological impairments. The participants were divided into three groups (control, sham control, and biofield intervention). Cognitive and motor function scores were assessed using the NIH Toolbox at baseline (day 0), day 90, and day 180. The biofield treatment group showed significant improvements in language function (p < 0.0001), working memory (p < 0.0001), and episodic memory (p < 0.0001) scores. Other cognitive functions also improved, although not statistically significant. The biofield intervention group also demonstrated significant enhancements (p < 0.05 to p < 0.0001) in locomotion, standing balance, dexterity, grip strength, and muscle endurance. No adverse effects were reported. The results suggest that remote biofield energy therapy is a safe, noninvasive intervention that improves cognitive and motor functions in adults. Further research is needed to understand its clinical benefits.
Violence and sharp force trauma in Nusaybin/Girnavaz Mound/Türkiye Late Roman–Early Islamic human remains
Authors: Köroğlu Tolga , Erol Ayla Sevim , Ocak Mert , Orhan Kaan 
Published: September 2024

AbstractGirnavaz Mound (Höyük), located in modern Türkiye, was studied between 1982 and 1991. The mound, inhabited from the Late Uruk Period to the Neo‐Assyrian Period, was later abandoned and repurposed as a cemetery during the Late Roman–Early Islamic Period. Anthropological studies on skeletons from this cemetery analyzed 148 individuals from this period, revealing notable trauma in five individuals. This study examines the skeletal remains of four individuals with perimortem sharp force trauma and one individual with an in situ arrowhead injury. Macroscopic and CT analyses indicated that both perimortem and antemortem trauma were intentional. The skull and body bones of four individuals displayed incision trauma likely inflicted by sword‐like cutting weapons, suggesting murder and decapitation, possibly as a form of execution. One individual had an arrowhead lodged in the tibia, indicating the person lived for some time with the injury and was buried with the arrowhead. This study aims to infer the period's political conflicts and cultural structures based on the trauma evidence. The findings suggest that the observed trauma may be linked to massacres and murders resulting from regional conflicts. This study offers exemplary insights into the mechanics and analysis of sharp force and perimortem trauma. Additionally, it sheds light on the social and historical context of the period.
Outcomes of proton therapy to infradiaphragmatic sites in pediatric patients with Hodgkin lymphoma
Authors: Jiang Cecilia , Kim Michele , Han Xiaoyan , Chelius Monica , Hoover Travis , Kersun Leslie , Reilly Anne F. , Hubbeling Harper , Cummings Elizabeth , Kurtz Goldie , Hill‐Kayser Christine , Plastaras John P. , LaRiviere Michael J. 
Published: September 2024

AbstractBackgroundProton therapy (PT) has potential advantages in pediatric Hodgkin lymphoma (pHL). However, there are limited data on PT, specifically to infradiaphragmatic targets. We report on PT planning details, doses achieved to organs at risk (OARs), and clinical and toxicity outcomes for patients with pHL who received PT to infradiaphragmatic regions.MethodsThis is a retrospective study including patients treated between 2011 and 2022. Demographic and clinical factors were collected, and toxicity was reported using Common Terminology Criteria for Adverse Events (CTCAE) version 5.0. Dosimetric and clinical factors associated with key outcomes were assessed via Cox regression. Photon plans were generated for all patients, and the paired t‐tests or Wilcoxon signed rank sum tests were used for dosimetric comparisons.ResultsTwenty‐one patients comprising 22 PT courses were included. Median follow‐up was 5.0 years, and mean age was 14.2 years. Median dose was 21 Gray equivalent (GyE) over 14 fractions. Top acute grade 1 (G1) toxicities included fatigue (59%) and anorexia (36%). Rates of acute G2 and G3+ toxicity were 18% and 0%, respectively. After PT, no local or marginal failures occurred. Five percent experienced disease progression, who were all successfully salvaged, and all patients were alive and disease‐free at last follow‐up. No secondary malignancies developed. Compared to photon radiotherapy, PT achieved significantly lower doses to the bowels, stomach, spleen, pancreatic tail, liver, kidneys, and pelvic bones.ConclusionsPT is well‐tolerated and leads to excellent oncologic and toxicity outcomes with long‐term follow‐up. PT confers dosimetric advantages when compared to photons.
Pylorus‐preserving pancreaticoduodenectomy with superior mesenteric vein resection and reconstruction in a child with recurrent hepatoblastoma after liver transplantation
Authors: Ziogas Ioannis A. , Gosain Ankush , Schulick Richard D. , Roach Jonathan P. , Del Chiaro Marco 
Published: September 2024

AbstractPancreaticoduodenectomy with vascular reconstruction is rarely performed in children. We present a 3‐year‐old male with stage IV hepatoblastoma and pre‐treatment extent of disease (PRETEXT) stage III with tumor into the portal vein and superior mesenteric vein (SMV), and with brain and lung metastases status post chemotherapy and stereotactic radiosurgery to left frontal brain lesion. He then underwent deceased donor liver transplant with Roux‐en‐Y hepaticojejunostomy complicated by two recurrences to bilateral lungs treated with wedge resections. His course lastly involved a third hepatoblastoma recurrence to the SMV that was managed with pylorus‐preserving pancreaticoduodenectomy with SMV resection and reconstruction.
Influence of alumina and PMMA on mechanical properties and aging behavior of 3D printed PLA composites: A comparative study
Authors: Nallamuthu Ramasamy , Thirugnanasamabandam Arunkumar , Kadirgama Kumaran , Chong William , Thangamani Geethapriyan , Alarifi Abdullah 
Published: September 2024

AbstractThis study intends to investigate the mechanical, thermal, and aging behaviors of 3D‐printed PLA (polylactic acid)‐blend with 10% polymethyl methacrylate (PMMA) and 10% alumina polymer composites for biomedical applications using compressive, DSC, and DMA analysis. The experimental results revealed that aged PLA blend with alumina samples increased compressive strength by 60.1% and 37.8% during hydrolytic and enzymatic degradation, respectively, compared to aged PLA samples. Also, it was reported that the PLA blend with PMMA samples increased compressive strength by 51.1% and 24% after hydrolytic and enzymatic degradation, respectively, as compared to aged PLA samples. Furthermore, DSC analysis revealed that alumina blended samples had a higher Tg than pure PLA and PMMA blended samples. In addition, DMA investigation revealed that the Tg of aged neat PLA, PLA/PMMA, and PLA/alumina increased by 4.38%, 4.8%, and 4.6%, respectively, compared to unaged polymer composites. Additionally, PLA/alumina‐aged samples exhibited stronger aging properties than neat PLA and PLA/PMMA blended‐aged samples. It was reported that the weight loss of PLA/Alumina was lowered by 10.7% and 15.6% compared to aged PLA/PMMA samples, for hydrolytic and enzymatic aging respectively. It was found that PLA alumina has better mechanical, thermal, and degradation resistance than PLA materials.Highlights
Alumina and PMMA materials were blended with PLA.
Examined the aging and mechanical properties of PLA blended composites.
Utilized hydrolytic and enzymatic aging for biomedical applications.
Evaluated mechanical strength performance of aged and unaged samples.
DSC and DMA were utlised for this research.
Performance and safety of volume guarantee ventilation in neonates using the fabian ventilator, a multicenter study
Authors: Molnar Zoltan , van Leuteren Ruud W. , Wilińka Maria , Szczapa Tomasz , Hutten Jeroen , Gupta Amit , Piwowarczyk Pawel , Wróblewska‐Seniuk Katarzyna , Chojnacka Karolina , van Kaam Anton H. , Belteki Gusztav 
Published: September 2024

AbstractObjectiveTo evaluate the performance (i.e., agreement between set and measured parameters) and safety (adverse events, device malfunctions, and ventilator alarms) of the fabian HFOi neonatal ventilator in volume guaranteed (VG) mode during conventional ventilation. To analyze the impact of leakage around the endotracheal tube and the set maximum allowed inflating pressure (Pmax).DesignProspective multicenter observational study.MethodsClinical and ventilator data were collected from 71 infants receiving VG ventilation for ≥12 h in four neonatal intensive care units (NICUs). Ventilator settings, parameters, and alarms were downloaded with 0.5 Hz sampling rate.ResultsData from 4,341 h of ventilation were analyzed. The median (interquartile range, IQR) of the absolute difference between the target and measured expired tidal volume was 0.76 (0.51–1.16) mL/kg. It was less when leak was <50% (median 0.36, IQR: 0.25–0.64 mL/kg, p < .001) and even less when the required peak inflating pressure (PIP) was also below Pmax (median: 0.09 mL/kg, IQR: 0.00–0.16 mL/kg, p < .001). On NICUs setting Pmax higher, tidal volume was maintained significantly closer to target. In 56 patients VG was continued until extubation. Two ventilator malfunctions were reported, none of them resulting in patient harm. “Tidal volume not reached” alarm occurred 32 times hourly, usually lasting for <10 s.ConclusionThe fabian HFOi ventilator maintains tidal volume close to its target, particularly when leak is <50% and when PIP is below Pmax. In most patients VG can be continued until extubation. Despite frequent ventilator alarms, ventilator malfunctions occur very rarely.