bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic variation, structural analysis, and virulence implications of BimA and BimC in clinical isolates of Burkholderia pseudomallei in Thailand
Authors: Cagape Charlene Mae Salao , Seng Rathanin , Saiprom Natnaree , Tandhavanant Sarunporn , Chewapreecha Claire , Boonyuen Usa , West T. Eoin , Chantratita Narisara 
Published: October 2024

AbstractMelioidosis is a life-threatening tropical disease caused by an intracellular gram-negative bacterium Burkholderia pseudomallei. B. pseudomallei polymerizes the host cell actin through autotransporters, BimA, and BimC, to facilitate intracellular motility. Two variations of BimA in B. pseudomallei have been reported previously: BimABp and BimA B. mallei-like (BimABm). However, little is known about genetic sequence variations within BimA and BimC, and their potential effect on the virulence of B. pseudomallei. This study analyzed 1,294 genomes from clinical isolates of patients admitted to nine hospitals in northeast Thailand between 2015 and 2018 and performed 3D structural analysis and plaque-forming efficiency assay. The genomic analysis identified 10 BimABp and 5 major BimC types, in the dominant and non-dominant lineages of the B. pseudomallei population structure. Our protein prediction analysis of all BimABp and major BimC variants revealed that their 3D structures were conserved compared to those of B. pseudomallei K96243. Sixteen representative strains of the most distant BimABp types were tested for plaque formation and the development of polar actin tails in A549 epithelial cells. We found that all isolates retained these functions. These findings enhance our understanding of the prevalence of BimABp and BimC variants and their implications for B. pseudomallei virulence.
A multi-objective fuzzy programming model for port tugboat scheduling based on the Stackelberg game
Authors: Ren Yangjun , Chen Qiong , Lau Yui-yip , Dulebenets Maxim A. , Li Botang , Li Mengchi 
Published: October 2024

AbstractTo solve the optimization problem of tugboat scheduling for assisting ships in entering and exiting ports in uncertain environments, this study investigates the impact of the decisions of tugboat operators and port dispatchers on tugboat scheduling under the scenario of dynamic task arrival and fuzzy tugboat operation time. Considering the features of the shortest distance tugboat principle, the first available tugboat principle, and the principle of fairness in the task volume of each tugboat, the tugboat company aims to minimize the total daily fuel consumption of tugboat operations, maximize the total buffer time of dynamic tasks, and minimize the total completion time as the objective functions. Due to the limitations of port vessel berthing and departure, as well as the allocation standards for piloting or relocating tugboats, the present study proposes a Stackelberg game-based fuzzy model for port tugboat scheduling with the tugboat operator and port dispatcher acting as decision makers at the upper and lower levels, respectively. A seagull optimization algorithm based on priority encoding and genetic operators is designed as a solution approach. CPLEX, genetic algorithm, standard seagull optimization algorithm, and simulated annealing algorithm are used to compare and analyze the solution results for the 45 problem cases generated from the actual data obtained from the Guangzhou Port. The results verify the efficiency of the proposed seagull optimization algorithm based on priority encoding and genetic operators. Furthermore, additional experiments are conducted to evaluate the changes in fairness coefficient, uncertain parameter correlation coefficients, and objective function correlation coefficients to demonstrate the practicality of the fuzzy programming model. This analysis involves adjusting the confidence level incrementally from 0 to 100% with respect to the model’s uncertain parameters.
The effect of laboratory diet and feeding on growth parameters in juvenile zebrafish
Authors: Hillman Courtney , Cooper Austin H. , Ram Pooja , Parker Matthew O. 
Published: October 2024

AbstractDespite being one of the most used laboratory species in biomedical, behavioral and physiological research, the nutritional requirements of zebrafish (Danio rerio) are poorly understood, and no standardized laboratory diet exists. Diet and feeding regimen can substantially impact the welfare of the fish and, in turn, experimental reproducibility. Consequently, the establishment of a standardized diet and feeding protocol for laboratory zebrafish is imperative to enhance animal welfare, guarantee research reproducibility and advance the economic and environmental sustainability of laboratory dietary practices. Here the aim of this systematic review is to provide an overview of the parameters that need to be standardized in future nutritional studies to facilitate future meta-analyses for confirmation of an optimal juvenile diet for growth. A comprehensive search was conducted in PubMed and Scopus to identify relevant studies published up to August 2023, and the studies were selected on the basis of the predefined inclusion/exclusion criteria. The databases yielded a total of 1,065 articles, of which 14 were included in this review. We conducted data extraction and risk-of-bias analysis in the included studies. Statistical comparisons for specific growth rate, weight gain (%) and length gain (%) parameters were performed to determine the optimal feed for enhanced juvenile growth. We identified significant heterogeneity and caveats to our findings owing to a lack of standardization of experimental conditions in nutritional studies. Our findings highlight an urgent need for research on zebrafish nutrition. Therefore, the standardized parameters we have reported here represent a critical starting point for studies.
The stringency and potential impact of climate laws and policies in the European Union and the 21OECD countries
Authors: Jahn Detlef 
Published: October 2024

AbstractIn this paper, I offer a way to measure the stringency of climate change legislation for macro-comparative studies using publicly available data. The paper is innovative in that it examines the complex interrelationship between national, subnational, and supranational legislation using data from the FaoLex database on environmental laws and policies. It is also novel in that it conducts a contextualized comparison that takes into account different national conditions for climate change policies. The resulting index is the Gross Potential Impact (GPI), which is a measure of the stimulus of legislation before taking into account implementation issues. To demonstrate the validity of the index, findings from case study research on the European Union’s 2020 and 2030 climate and energy frameworks are used. The rest of the paper presents the GPI for 21 OECD countries for the period between 1990 and 2020.
Binary neutron star merger offsets from their host galaxies
Authors: Gaspari N. , Stevance H. F. , Levan A. J. , Chrimes A. A. , Lyman J. D. 
Published: October 2024

Aims. The locations of binary neutron star (BNS) mergers within their host galaxies encode the systemic kicks that these systems received in the supernova aftermath. We investigate how the galactic potential and the systemic kicks shape the offset distribution of BNS mergers with a case study of GW 170817 and its host NGC 4993.
Methods. We derived dynamical constraints on the host potential from integral field spectroscopy with Jeans anisotropic modelling. We evolved the trajectories of synthetic BNSs from the BPASS code in the galactic potential, using two different kick prescriptions to investigate how the observed offsets might differentiate between these two possibilities. The simulation was repeated after swapping the host potential with that of a dwarf galaxy, to test the effect of the potential on the offsets.
Results. The location of GW 170817 is entirely consistent with our predictions, regardless of large or small kicks, because the strong potential of NGC 4993 is only diagnostic of very large kicks. In galaxies of similar or greater mass, large offsets can constrain large kicks, while small offsets do not provide much information. In an old dwarf galaxy, on the other hand, small offsets can constrain small kicks, while large offsets would prevent host association.
Machine learning methods for automated interstellar object classification with LSST
Authors: Cloete Richard , Vereš Peter , Loeb Abraham 
Published: October 2024

Context. The Legacy Survey of Space and Time (LSST), to be conducted with the Vera C. Rubin Observatory, is poised to revolutionize our understanding of the Solar System by providing an unprecedented wealth of data on various objects, including the elusive interstellar objects (ISOs). Detecting and classifying ISOs is crucial for studying the composition and diversity of materials from other planetary systems. However, the rarity and brief observation windows of ISOs, coupled with the vast quantities of data to be generated by LSST, create significant challenges for their identification and classification.
Aims. This study aims to address these challenges by exploring the application of machine learning algorithms to the automated classification of ISO tracklets in simulated LSST data.
Methods. We employed various machine learning algorithms, including random forests (RFs), stochastic gradient descent (SGD), gradient boosting machines (GBMs), and neural networks (NNs), to classify ISO tracklets in simulated LSST data.
Results. Our results demonstrate that GBM and RF algorithms outperform SGD and NN algorithms in accurately distinguishing ISOs from other Solar System objects. RF analysis shows that many derived Digest2 values are more important than direct observables (right ascension, declination, and magnitude) in classifying ISOs from the LSST tracklets. The GBM model achieves the highest precision, recall, and F1 score, with values of 0.9987, 0.9986, and 0.9987, respectively.
Conclusions. These findings lay the foundation for the development of an efficient and robust automated system for ISO discovery using LSST data, paving the way for a deeper understanding of the materials and processes that shape planetary systems beyond our own. The integration of our proposed machine learning approach into the LSST data processing pipeline will optimize the survey’s potential for identifying these rare and valuable objects, enabling timely follow-up observations and further characterization.
Two stellar populations with different metallicities in the low-mass globular cluster Gran 5
Authors: Lim Dongwook , Chun Sang-Hyun , Lee Young-Wook , Chung Chul , Koch-Hansen Andreas J. , Hong Seungsoo 
Published: October 2024

Context. With the increasing number of discoveries of globular clusters in the inner Milky Way, the need for spectroscopic confirmation and further investigation of their stellar populations and chemodynamical properties has become crucial.
Aims. Gran 5 is a newly reported low-mass globular cluster located close to the Galactic center, and it is thought to be an accreted object associated with the Gaia-Enceladus structure. This study aims to investigate the stellar populations of Gran 5 and their detailed chemical properties.
Methods. We performed high-resolution near-infrared spectroscopy on seven stars in the field of Gran 5 using IGRINS on the Gemini-South telescope.
Results. We identified six stars as cluster members and reveal that they are divided into two stellar populations with different metallicities, with mean [Fe/H] values of −0.76 dex and −0.55 dex, respectively. In addition, the chemodynamical properties of Gran 5 agree with those of in situ globular clusters.
Conclusions. Our findings represent the first detection of two stellar populations with different metallicities in a low-mass globular cluster. This suggests that the metallicity variation in Gran 5 may have arisen from processes different from those in other globular clusters with metallicity variation, or that it may have lost a substantial amount of its initial mass during its evolution.
Studying the X-ray absorption characteristics of Centaurus X-3 using nearly 14 years of MAXI/GSC data
Authors: Balu Ajith , Roy Kinjal , Manikantan Hemanth , Tamang Abhisek , Paul Biswajit 
Published: October 2024

Context. Centaurus X-3 is a persistent high-mass X-ray binary with the long-term light curve from the source exhibiting orbit-to-orbit intensity variations with no apparent superorbital periodicity.
Aims. We used ∼13.5 years of MAXI/GSC data to study the long-term behaviour of X-ray absorption caused by the stellar wind from the companion star and any absorbing structures present in the binary.
Methods. We used orbital-phase-resolved spectroscopy to study the variation in the photoelectric absorption along the line of sight of the source for both the intensity-averaged data and intensity-resolved data after dividing all the data binned with orbital period into three intensity levels.
Results. We find an asymmetric variation in the photoelectric absorption along the line of sight across an orbit of the source. The orbital-phase-resolved spectra show a clear increase in photoelectric absorption after ϕorb ∼ 0.5, which deviates from a spherically symmetric stellar wind model. The flux of Cen X-3 shows significant variation between consecutive orbits. An intensity-resolved spectral analysis of the source was performed, followed by an intensity-resolved and orbital-phase-resolved spectral analysis, which showed that at the medium and high intensity levels, the orbital-phase-resolved photoelectric absorption is slightly asymmetric with respect to mid-phase (ϕorb = 0.5). The asymmetry is very pronounced at the lowest intensity level and cannot be explained by a spherically symmetric wind from the companion star.
Conclusions. The differences in the orbital phase-dependence of absorption for different intensity levels suggest that the presence of an accretion wake, photoionization wake, or tidal stream is more prominent at a lower intensity level for Centaurus X-3 than at a higher intensity level.
Investigations on the Hemostatic Potential of Physiological Body Fluids
Authors: Thaler Johannes , Tripisciano Carla , Nieuwland Rienk 
Published: October 2024

AbstractCurrent blood coagulation models consider the interactions between blood, the vessel wall, and other tissues that expose tissue factor (TF), the main initiator of coagulation. A potential role of body fluids other than blood is generally not considered. In this review, we summarize the evidence that body fluids such as mother's milk saliva, urine, semen, and amniotic fluid trigger coagulation. The ability of these body fluids to trigger coagulation is explained by the presence of extracellular vesicles (EVs). These EVs expose extrinsic tenase complexes (i.e., complexes of TF and activated factor VII) that can trigger coagulation. Why these body fluids share this activity, however, is unknown. Possible explanations are that these body fluids contribute to hemostatic protection and/or to the regulation of the epithelial barrier function. Further investigations may help understand the underlying cellular and biochemical pathways regulating or contributing to coagulation and innate immunity, which may be directly relevant to medical conditions such as gastrointestinal bleeding and chronic inflammatory bowel disease.
Update on Tissue Factor Detection in Blood in 2024: A Narrative Review
Authors: Bonifay Amandine , Cointe Sylvie , Plantureux Léa , Lacroix Romaric , Dignat-George Françoise 
Published: October 2024

AbstractTissue factor (TF) is a transmembrane protein essential for hemostasis. Different forms of active TF circulate in the blood, either as a component of blood cells and extracellular vesicles (EVs) or as a soluble plasma protein. Accumulating experimental and clinical evidence suggests that TF plays an important role in thrombosis. Many in-house and commercially available assays have been developed to measure TF-dependent procoagulant activity or antigen in blood and have shown promising results for the prediction of disease outcomes or the occurrence of thrombosis events in diseases such as cancer or infectious coagulopathies. This review addresses the different assays that have been published for measuring circulating TF antigen and/or activity in whole blood, cell-free plasma, and EVs and discusses the main preanalytical and analytical parameters that impact results and their interpretation, highlighting their strengths and limitations. In the recent decade, EVTF assays have been significantly developed. Among them, functional assays that use a blocking anti-TF antibody or immunocapture to measure EVTF activity have higher specificity and sensitivity than antigen assays. However, there is still a high variability between assays. Standardization and automatization are prerequisites for the measurement of EVTF in clinical laboratories.
Alles Gluten, oder was? Weizenallergie – Zöliakie – Glutensensitivität
Authors: Schmidt Robert 
Published: October 2024

ZusammenfassungImmer mehr Menschen meinen, Weizen bzw. Gluten nicht zu vertragen. Nicht nur Patienten mit gastrointestinalen Beschwerden, sondern auch mit Haut- und Gelenkbeschwerden oder unspezifischen Symptomen wie Müdigkeit, Abgeschlagenheit und Konzentrationsstörungen führen dies immer häufiger auf ihre Ernährungsgewohnheiten zurück. Dabei geht es natürlich nicht nur – aber oft – um das Thema Weizen bzw. Gluten. Da letztendlich nur die genaue Diagnose entscheidet, ob überhaupt und wenn ja wie konsequent eine bestimmte Diät einzuhalten ist, lohnt es sich, sich die 3 häufigen Beschwerdebilder Weizenallergie, Zöliakie und Glutensensitivität nochmals differenziert zu vergegenwärtigen. Bei der Weizenallergie handelt es sich um eine Überreaktion des Immunsystems, bei der Zöliakie um eine chronische Autoimmunerkrankung der Dünndarmschleimhaut, und der Verdacht auf eine Weizen- oder Glutensensitivität erhärtet sich, wenn die unspezifischen Beschwerden wie Reizdarmsyndrom nach Ausschluss einer Weizenallergie oder Zöliakie bestehen bleiben. Jedes Krankheitsbild erfordert eine unterschiedliche Auslassdiät von weizen- und glutenhaltigen Lebensmitteln.
Homöopathische Arzneimittel bei nahrungsinduzierten Beschwerden
Authors: Boeddrich Ute , Elies Michael K. H. 
Published: October 2024

ZusammenfassungZunehmend suchen Patientinnen und Patienten mit Beschwerden, denen mutmaßlich Allergien und/oder Unverträglichkeiten von Nahrungsmitteln zugrunde liegen, die naturheilkundliche Praxis auf. Bei nahrungsinduzierten Beschwerden wie Verdauungsstörungen, Übelkeit oder Blähungen stehen diverse homöopathische Mittel zur Verfügung, um das Gleichgewicht im Körper wiederherzustellen und die Selbstheilungskräfte zu aktivieren. Oft liegen den Unverträglichkeiten verschiedene Auslöser oder Ursachen zugrunde, die nach sorgfältiger Anamnese und Konstitution gefunden werden müssen. Der Beitrag geht auf den Stellenwert und die Möglichkeiten homöopathischer Arzneimittel für die erfahrungsheilkundliche Therapie ein.
An annotated checklist of Syzygium subgenus Sequestratum (Myrtaceae)
Authors: Lyytikäinen Virva M. M. , Hatt Sebastian A. , Low Yee Wen , Burslem David F. R. P. , Lucas Eve J. 
Published: October 2024

We present an annotated checklist of the 29 species comprising Syzygium subg. Sequestratum. Species in the subgenus were identified through a comprehensive review of herbarium specimens and relevant literature, including both historic taxonomic and regional revisions of Syzygium, and recent molecular phylogenetic analyses. No single character is diagnostic of subg. Sequestratum; instead, a combination of commonly occurring characters is used, including glaucescence in the hypanthium, funnel-shaped flowers <10 mm long and coriaceous leaves with dark drying petioles in contrast to a paler lamina. Species are distributed across East Asia, Malesia and Eastern Australia, with an apparent preference for nutrient-poor habitats. This checklist provides a foundation for future taxonomic revision of this clade, from an early branching node in the tree of life of the world’s largest tropical tree genus.
Zero-dimensional simulations of DC ns-pulsed plasma jet in N2 at near atmospheric pressure: validation of the vibrational kinetics
Authors: He Youfan , Kuhfeld Jan , Lepikhin Nikita D , Czarnetzki Uwe , Guerra Vasco , Peter Brinkmann Ralf , Gibson Andrew R , Kemaneci Efe 
Published: October 2024

Abstract
A Direct current (DC) nanosecond (ns)-pulsed plasma jet fed with N2 at near atmospheric pressure (

20

000

Pa) is studied using a transient zero-dimensional (0-D) model coupled with a two-term Boltzmann equation solver. Good agreement is observed between the simulated and measured plasma properties: including the electron density and the ratios of the N

2

(
v
=
1
,
2
,
3
,
4
)

densities to the gas density. A variety of theoretical approaches are considered to determine the Vibrational-Vibrational (V-V) and Vibrational-Translational (V-T) rate coefficients. For the V-V kinetics, the simple form of an Harmonic Oscillator (sfHO), the Schwartz-Slawsky-Herzfeld (SSH) and the Forced Harmonic Oscillator (FHO) approaches are used. The SSH approach used in this study is an improved version based on the pure SSH approach. For the V-T kinetics, the sfHO, a fit function of the Semi-Classical (ffSC) calculations and a fit function of the Quasi-Classical Trajectory (ffQCT) calculations are used. The influence of these different approaches on the calculated temporal evolution profiles of the vibrational distribution functions (VDFs) within one pulse modulation cycle is revealed. It is observed that the use of these different approaches does not strongly affect the densities of the low vibrational levels (v < 5), whereas larger influences on the higher level densities are found. In addition, it is found that the simulated evolution of the VDFs are sensitive to the probabilities of the neutral wall reaction

N

2

(
v
)
+

wall

→

N

2

(
v
−
1
)

in the range of

1
×

10

−
2

to

1
×

10

−
4

. A further analysis of the wall quenching probabilities of

N

2

(
0
<
v
<
58
)

is of importance for a more accurate prediction of the VDFs.
A 1D micromechanics-based constitutive model for the thermoviscoelastic behavior of crosslinked semicrystalline shape memory polymers: numerical simulation and experimental validation
Authors: Zeng Hao , Shi Jiawen , Sun Huiyu , Gu Jianping , Xu Xiaotao , Song Linhui 
Published: October 2024

Abstract
The paper develops a 1D thermoviscoelastic constitutive model for the crystallization- and melting-induced one-way and two-way shape memory effects, as well as isothermal yielding behaviors, of crosslinked semi-crystalline polymers. A micromolecular chain model is proposed to characterize the transition between the amorphous and crystalline phases. Structural equations including a modified Eying model that combine phase transition and viscoelasticity equations are employed to predict the shape memory effects. An extensive experimental campaign has been carried out on poly (ethylene-co-vinyl acetate) based semi-crystalline elastomers to characterize the thermoviscoelastic temperature-stress-strain relations of the material under different loading and rate conditions. Some results guide the determination of the model parameters, while the rest validate the model capabilities. Comparisons with the experimental results show that the model can well reproduce the stress-strain-temperature responses, providing valuable insights for application development.