bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application
Authors: Qi Ying-Xin , Yao Qing-Ping , Huang Kai , Shi Qian , Zhang Ping , Wang Guo-Liang , Han Yue , Bao Han , Wang Lu , Li Hai-Peng , Shen Bao-Rong , Wang Yingxiao , Chien Shu , Jiang Zong-Lai 
Published: April 2016

SignificanceThe proliferation of vascular smooth muscle cells (VSMCs) in response to excessive cyclic stretch is crucial in vascular remodeling in hypertension. To elucidate the molecular mechanism, we studied the mechanobiological roles of emerin and lamin A/C, two important components of nuclear envelope proteins localized beneath the inner nuclear membrane. We found that emerin and lamin A/C play significant roles in the mechanical modulation of VSMC proliferation. The repressed expression of emerin and lamin A/C mediates the stretch-induced VSMC proliferation, which is important in vascular remodeling during hypertension. Emerin and lamin A/C bind to the respective sequencing-specific motifs of transcription factors to mediate the molecular mechanisms underlying the hyperstretch-induced VSMC dysfunction.
Mechanism of APC/C
CDC20
activation by mitotic phosphorylation
Authors: Qiao Renping , Weissmann Florian , Yamaguchi Masaya , Brown Nicholas G. , VanderLinden Ryan , Imre Richard , Jarvis Marc A. , Brunner Michael R. , Davidson Iain F. , Litos Gabriele , Haselbach David , Mechtler Karl , Stark Holger , Schulman Brenda A. , Peters Jan-Michael 
Published: April 2016

Significance
The ability of eukaryotic cells to pass their genomes properly from one cell generation to the next depends on the 1.2-MDa ubiquitin ligase complex APC/C (anaphase-promoting complex/cyclosome) and on the correct timing of its activation by the substrate adaptor CDC20 (cell division cycle 20). Although it has been known for two decades that mitotic APC/C phosphorylation is required for its activation by CDC20, the mechanistic basis of this process remained unknown, in part because the existence of numerous phospho-sites on APC/C made systematic mutagenesis approaches difficult. Here we have used the biGBac technique for the rapid assembly of multigene expression constructs to overcome this limitation and discovered that APC/C contains an autoinhibitory loop region that prevents CDC20 binding until it becomes phosphorylated in mitosis.
biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
Authors: Weissmann Florian , Petzold Georg , VanderLinden Ryan , Huis in 't Veld Pim J. , Brown Nicholas G. , Lampert Fabienne , Westermann Stefan , Stark Holger , Schulman Brenda A. , Peters Jan-Michael 
Published: April 2016

Significance
At the molecular level, most processes in living systems are mediated by multisubunit protein complexes. Recombinant forms of these complexes are essential for analyzing their structure and function. Multigene expression constructs greatly improve recombinant protein complex preparations, but the generation of such constructs can be a rate-limiting step. To overcome this limitation, we have adapted Gibson assembly reactions for the rapid, efficient, and fast generation of numerous expression constructs in parallel and used the resulting biGBac method for expression of different cell-cycle complexes, composed of up to 17 different subunits. The biGBac technique enables the analyses of large protein complexes by systematic mutagenesis approaches that were not feasible before.
Emergence of a new predator in the North Sea: evaluation of potential trophic impacts focused on hake, saithe, and Norway pout
Authors: Cormon Xochitl , Kempf Alexander , Vermard Youen , Vinther Morten , Marchal Paul 
Published: April 2016

Abstract
During the last 15 years, northern European hake (Merluccius merluccius) has increased in abundance, and its spatial distribution has expanded in the North Sea region in correlation with temperature. In a context of global warming, this spatial shift could impact local trophic interactions: direct impacts may affect forage fish through modified predator–prey interactions, and indirect impacts may materialize through competition with other resident predators. For instance, North Sea saithe (Pollachius virens) spatial overlap with hake has increased while saithe spawning-stock biomass has decreased recently notwithstanding a sustainable exploitation. In this context, we investigated the range of potential impacts resulting from most recent hake emergence in the North Sea, with a particular focus on saithe. We carried out a multispecies assessment of North Sea saithe, using the Stochastic MultiSpecies (SMS) model. In addition to top-down processes already implemented in SMS, we built in the model bottom-up processes, relating Norway pout (Trisopterus esmarkii) abundance and saithe weight-at-age. We simulated the effects, on all North Sea species being considered but focusing on Norway pout and saithe, of combining different hake abundance trends scenarios with the inclusion of bottom-up processes in SMS. North Sea saithe FMSY was then evaluated in a multispecies context and contrasted with single-species value. The different scenarios tested revealed a negative impact of hake emergence on saithe biomass, resulting from an increase of predation pressure on Norway pout. These results confirm the competition assumption between saithe and hake in the North Sea and might partially explain the most recent decrease of saithe biomass. This study also highlighted that taking into account bottom-up processes in the stock assessment had a limited effect on the estimation of saithe FMSY which was consistent with single-species value.
Did Garin and Bujadoux Actually Report a Case of Lyme Radiculoneuritis?
Authors: Wormser Gary P. , Wormser Vanessa 
Published: April 2016

Abstract
A 1922 report by Garin and Bujadoux is widely regarded as describing the first case of neurologic Lyme borreliosis. Although the patient reported had a tick bite followed by the development of a rash and radiculoneuritis, there were a number of highly atypical features, raising the question of whether the patient, in fact, had neurologic Lyme borreliosis. The paper may not deserve the historic recognition that it has received.
Mechanisms of Familial Influence on Reentry among Formerly Incarcerated Latino Men
Authors: Lee Jane Jean-Hee , Guilamo-Ramos Vincent , Muñoz-Laboy Miguel , Lotz Kevin , Bornheimer Lindsay 
Published: April 2016

Abstract
In the United States more than 10,000 people are released from state and federal prisons every week and often reenter the communities in which they were arrested. Formerly incarcerated individuals face considerable challenges to securing employment and housing. Subsequently, approximately two-thirds of former prisoners are rearrested within three years of their release. Latino men represent the fastest growing ethnic group of prisoners in the United States with unique cultural and social needs during the reentry process. The present study examined the role of the family in the reentry process through in-depth interviews (N = 16) with formerly incarcerated Latino men (FILM). The authors sought to identify familial processes specific to Latino men with potential to affect engagement and participation in reentry programs. Findings suggest that family mechanisms of social control and social support influence FILM’s reentry. Social work practitioners who work with this growing population can engage familial processes to prevent recidivism and promote desistance.
Geometric Torsion in Adolescent Idiopathic Scoliosis
Authors: Shen Jesse , Kadoury Samuel , Labelle Hubert , Parent Stefan 
Published: April 2016

Study Design.
Consecutive case series analysis.

Objective.
To evaluate the surgical outcomes of patients with thoracic adolescent idiopathic scoliosis (AIS) in relation to different degrees of geometric torsion.

Summary of Background Data.
AIS is a three-dimensional (3D) deformity of the spine. A 3D classification of AIS, however, remains elusive because there is no widely accepted 3D parameter in the clinical practice. Recently, a new method of estimating geometric torsion has been proposed and detected two potential new 3D subgroups based on geometric torsion values.

Methods.
This is an analysis of 93 patients with Lenke type-1 deformity from our institution. 3D reconstructions were obtained using biplanar radiographs both pre- and postoperatively. Geometric torsion was computed using a novel technique by approximating local arc lengths at the neutral vertebra in the thoracolumbar segment. An inter- and intragroup statistical analysis was performed to compare clinical indices of patients with different torsion values. A qualitative assessment was also performed on each patient by two senior staff surgeons.

Results.
Statistically significant differences were observed in clinical indices between high (2.85 mm−1) and low torsion (0.83 mm−1) Lenke type 1 subgroups. Preoperatively, the high torsion group showed higher Cobb angle values in the thoracic segment (71.18° vs. 63.74°), as well as higher angulation in the thoracolumbar plane of maximum deformity (67.79° vs. 53.30°). Postoperatively, a statistically significant difference was found in the orientation of the plane of maximum deformity in the thoracolumbar segment between the high and low torsion groups (47.95° vs. 30.03°). Results from the qualitative evaluation of surgical results showed different results between the two staff surgeons.

Conclusion.
These results suggest a link between preoperative torsion values and surgical outcomes within Lenke type 1 deformities. These results will need to be validated by an independent group, as it is a single-center study.

Level of Evidence: 4
Efficacy and tolerability of bazedoxifene in Mexican women with osteoporosis: a subgroup analysis of a randomized phase 3 trial
Authors: Palacios Santiago , Williams Robert , Mirkin Sebastian , Pan Kaijie , Arias Lizbeth , Komm Barry S. 
Published: April 2016

Abstract

Objective:
Bazedoxifene (BZA) is a selective estrogen receptor modulator that reduces fracture risk and bone turnover in postmenopausal women with osteoporosis. This analysis evaluated BZA's effects on bone mineral density (BMD) and bone turnover in Mexican women with osteoporosis from the global pivotal trial (Study Evaluating Bazedoxifene Acetate in Osteoporosis in Postmenopausal Women).

Methods:
In this 3-year, phase 3, randomized, double-blind trial, healthy postmenopausal women with osteoporosis (N = 7,492) received BZA 20 or 40 mg/d, raloxifene 60 mg/d, or placebo. The subanalyses of Mexican women assessed serum concentrations of osteocalcin and collagen type 1 C-telopeptide, BMD, and tolerability with BZA 20 mg/d versus placebo.

Results:
In the Mexican subgroup (BZA, n = 39; placebo, n = 37) at month 12, BZA 20 mg/d produced significant (P < 0.001) percentage decreases from baseline in osteocalcin (−40.5 vs −18.5) and C-telopeptide (−45.7 vs −29.4). For BZA versus placebo, percentage change in BMD from baseline to month 36 was 3.3 versus 0.64 for lumbar spine, −0.18 versus −1.8 for total hip, 0.21 versus −2.6 for femoral neck, and −0.55 versus −1.4 for femoral trochanter; differences were not statistically significant. Results were comparable to the overall study population in which differences were statistically significant. Common adverse events (≥20%) included arthralgia, back pain, gastritis, headache, influenza, and pain; none led to study withdrawal.

Conclusions:
In Mexican women with osteoporosis, BZA was well tolerated and seems to produce BMD changes comparable to the global phase 3 population, although differences versus placebo were not statistically significant in this smaller subgroup.
Switch Flap for Upper Eyelid Reconstruction—How Soon Should the Flap Be Divided?
Authors: Uemura Tetsuji , Yanai Tetsu , Yasuta Masato , Kawano Hiroshige , Ishihara Yasuhiro , Kikuchi Mamoru 
Published: April 2016

Background:
The results of a cohort of patients treated at one institution for upper eyelid reconstruction with the switch flap method after a defect due to excision of malignant tumor were reviewed.

Methods:
A retrospective data file review of all patients who had undergone total upper eyelid reconstruction with the switch flap method was conducted at the Saga University Hospital between April 2000 and October 2014. The follow-up lasted for varying periods during which the preoperative and postoperative photographs were compared as well.

Results:
A total of 10 patients with upper eyelid tumors, that is, 7 sebaceous carcinoma, 2 squamous cell carcinoma, and 1 basal cell carcinoma, underwent reconstructive surgery. With the switch flap technique, the defects resulting from tumor excision were completely covered in all cases. The mean of defect widths after tumor excision (A) was 18.8 mm (range, 15–25 mm), the mean of widths of switch flaps (B) was 13.3 mm (range, 8–22 mm), and the mean of B/A ratios was 0.69 (range, 0.5–0.88). When the switch flap was divided at 7 to 14 days, there was no flap loss, trichiasis, or corneal ulcer.

Conclusion:
Our protocol managed to make flaps with a B/A ratio of 0.5–0.7, and the flaps were divided at 7 to 14 days after surgery, the timing of which was much earlier than in the conventional method, lessening the possibility of complications.
Patient Expectations of Bariatric and Body Contouring Surgery
Authors: Poulsen Lotte , Klassen Anne , Jhanwar Sabrina , Pusic Andrea , Roessler Kirsten K. , Rose Michael , Sørensen Jens Ahm 
Published: April 2016

Background:
Patient expectations are important in bariatric and body contouring surgery because the goals include improvements in health-related quality of life, appearance, and body image. The aim of this study was to identify patient expectations along the weight loss journey and/or body contouring surgery.

Methods:
This qualitative study took an interpretive description approach. Between September 2009 and February 2012, 49 patients were interviewed postbody contouring surgery. Data were analyzed using a line-by-line approach whereby expectations were identified and labeled as expected, unexpected, or neutral. Constant comparison was used to ensure coding was done consistently. Interviews continued until no new themes emerged.

Results:
Participants described expectations according to appearance, health-related quality of life, and patient experience of care. Two areas stood out in terms of unmet expectations and included appearance and physical health, ie, recovery from body contouring surgery. Most participants, who underwent bariatric surgery, expected neither the extent of excess skin after weight loss nor how the excess skin would make them look and feel. For recovery, participants did not expect that it would be as long or as hard as it was in reality.

Conclusions:
A full understanding of outcomes and expectations for this patient population is needed to enhance patient education and improve shared medical decision making. Education materials should be informed by the collection of evidence-based patient-reported outcome information using measures such as the BODY-Q. A patient-reported outcome scale measuring patient expectations is needed for obese and bariatric patients.
Health-Related Quality of Life after Minimally Invasive Cardiac Surgery
Authors: Holmes Sari D. , Fornaresio Lisa M. , Shuman Deborah J. , Pritchard Graciela , Ad Niv 
Published: April 2016

Objective Factors influencing health-related quality of life (HRQL) after minimally invasive cardiac surgery have not been well described. We examined the trajectory of HRQL after minimally invasive cardiac surgery and the role of perioperative factors and rhythm on HRQL changes. Methods Patients underwent minimally invasive surgical ablation for atrial fibrillation and/or valve surgery (n = 235). Health-related quality of life (SF-12) and clinical status were assessed preoperatively and postoperatively. Results Physical summary HRQL (F = 36.2, P < 0.001) and mental summary HRQL (F = 3.2, P = 0.047) improved significantly by 12 months after surgery. Improvement on HRQL peaked at 6 months and plateaued between 6 and 12 months. Physical HRQL was similar to age-based normal values before surgery (P = 0.66) and surpassed norms by 6 months after surgery (P < 0.001). Younger age (r = −0.15, P = 0.02) and lower EuroSCORE II (r = −0.19, P = 0.003) correlated with greater HRQL improvements by 6 months. Only lower EuroSCORE II (r = −0.14, P = 0.04) correlated with greater HRQL improvement by 12 months. Length of stay and major morbidity were not related to HRQL improvement. In surgical ablation patients, restoration of stable sinus rhythm throughout the first 12 months was associated with greater physical HRQL improvement by 6 months compared with patients who had atrial arrhythmia recurrences (change, 5.0 vs. −1.0, P = 0.02). Conclusions Health-related quality of life improved significantly after minimally invasive cardiac surgery. These improvements were influenced by age, operative risk, symptoms, and rhythm status. Even patients with HRQL in a normal range before surgery can experience improved HRQL after surgery. Minimally invasive cardiac surgery can offer decreased postoperative complications and also improved HRQL.
Short article: Management of ruptured hepatocellular carcinoma in a European tertiary care center
Authors: Rijckborst Vincent , ter Borg Martijn J. , Tjwa Eric T. , Sprengers Dave , Verhoef Kees , Moelker Adriaan , Ijzermans Jan N. , de Man Robert A. 
Published: April 2016

Goals and background
Spontaneous rupture is a rare complication of hepatocellular carcinoma (HCC). Treatment options consist of transcatheter arterial embolization (TAE), hepatic resection, and conservative therapy. The best approach is under debate.

Study
This study presents a review of clinical data of patients with a ruptured HCC admitted to a European tertiary care center.

Results
Eleven patients were included; six (55%) had underlying cirrhosis. The majority of patients (73%) had no previous history of HCC. Spontaneous HCC rupture was diagnosed using abdominal computed tomography with or without a diagnostic paracentesis. Computed tomography showed one or two tumors in eight (73%) patients; the other patients had multiple tumors or diffuse infiltrative HCC. Seven (64%) patients were initially treated by TAE and one (9%) patient underwent hepatic resection. The remaining three (27%) patients, all of whom had liver cirrhosis, received conservative therapy. Two patients initially treated by TAE underwent a delayed resection and ultimately received systemic therapy. Overall, at the end of the follow-up period, three patients were still alive at 84, 991, and 1026 days after the initial presentation. Eight (73%) patients had died after a median of 88 days (range 7–417). One year after presentation, none of the conservatively treated patients was alive compared with three out of seven (43%) patients treated with TAE with or without delayed resection.

Conclusion
Patients with a spontaneously ruptured HCC have a poor prognosis. In selected patients, however, prolonged survival is possible using TAE as initial therapy with or without a delayed resection and systemic therapy.
Effect of a Pragmatic, Cluster-randomized Controlled Trial on Patient Experience With Care
Authors: Dorr David A. , Anastas Tracy , Ramsey Katrina , Wagner Jesse , Sachdeva Bhavaya , Michaels LeAnn , Fagnan Lyle J. 
Published: April 2016

Background:
Health reform programs like the patient-centered medical home are intended to improve the triple aim. Previous studies on patient-centered medical homes have shown mixed effects, but high value elements (HVEs) are expected to improve the triple aim.

Objective:
The aim of this study is to understand whether focusing on HVEs would improve patient experience with care.

Methods:
Eight clinics were cluster-randomized in a year-long trial. Both arms received practice facilitation, IT-based reporting, and financial incentives. Intervention practices were encouraged to choose HVEs for quality improvement goals. To assess patient experience, 1597 Consumer Assessment of Healthcare Providers and Systems surveys were sent pretrial and posttrial to a stratified random sample of patients. Difference-in-difference multivariate analysis was used to compare patient responses from intervention and control practices, adjusting for confounders.

Results:
The response rate was 43% (n=686). Nonrespondent analysis showed no difference between arms, although differences were seen by risk status and age. The overall difference in difference was 2.8%, favoring the intervention. The intervention performed better in 9 of 11 composites. The intervention performed significantly better in follow-up on test results (P=0.091) and patients’ rating of the provider (P=0.091), whereas the control performed better in access to care (P=0.093). Both arms also had decreases, including 4 of 11 composites for the intervention, and 8 of 11 for the control.

Discussion:
Practices that targeted HVEs showed significantly more improvement in patient experience of care. However, contemporaneous trends may have affected results, leading to declines in patient experience in both arms.
Refractory Mononeuritis Multiplex Due to Hepatitis C Infection and Cryoglobulinemia
Authors: Soares Cristiane N. 
Published: April 2016

Background:
Mononeuritis multiplex due to hepatitis C infection and cryoglobulinemia has no specific treatment guidelines. Despite the favorable evolution of the liver disease after treatment with interferon and ribavirin, neurological symptoms might not respond very efficiently to antiviral therapy.

Case Report:
We report the case of a 50-year-old woman, with a mononeuritis multiplex related to cryoglobulinemia and hepatitis C virus infection, who was treated with rituximab. Hepatitis C virus infection was treated successfully with interferon-α and ribavirin, but the neurological symptoms were still worsening until rituximab therapy. Significant improvement of paraparesis and painful hypoesthesia were evident after the fourth infusion of rituximab. However, every 6 months, the neurological symptoms relapsed and the patient was subjected to a new cycle of rituximab therapy, with the disappearing of the paraparesis and hypoesthesias.

Conclusions:
This case highlights the potential use of rituximab in immune-mediated neuropathies, especially the mononeuritis multiplex associated with hepatitis C infection.