bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Essential Oils of Schinus molle L. Leaves Growing in Different Regions of Algeria in Terms of Their Chemical Compositions and Various Biological Activities
Authors: Kouachi Amina , Madani Zohra , MeltemTaş‐Küçükaydın  , Eroğlu Büşra , Korkmaz Cansu , Belhouala Khadidja , Küçükaydın Selçuk , Aouiche Adel , Ceylan Özgür , Duru Mehmet Emin , Benarba Bachir 
Published: October 2024

ABSTRACTThis preliminary study investigated the chemical composition and biological properties of essential oils derived from Schinus molle L. leaves obtained from three specific regions in Algeria: Algiers (SMA), Djelfa (SMD), and Mascara (SMM). Gas chromatography (GC) and Gas chromatography–Mass Spectrometry (GC–MS) analyses confirmed the presence of 52 compounds (100.0%) in SMA essential oil, 50 compounds (100.0%) in SMD, and 72 compounds (100.0%) in SMM essential oils. The main components of SMA oil were camphene (31.82%), limonene (14.71%), and p‐cymene (9.25%). In SMD and SMM oils, α‐phellandrene (14.25% and 12.70%), limonene (13.02% and 11.90%), and germacrene D (10.62% and 10.15%) were the major components, respectively. The antioxidant characteristics were evaluated using five methods: β‐carotene‐linoleic acid, DPPH, ABTS+, CUPRAC, and Metal chelating assays. The results revealed a moderate to low anti‐oxidant effect, with SMA essential oils exhibiting the highest activity. A moderate inhibitory effect against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α‐glucosidase, α‐amylase, urease, and tyrosinase was observed, indicating anti‐enzymatic activity. Nevertheless, all samples had higher IC50 values for both antioxidant and anti‐enzymatic activities than 200 μg/mL. It was determined that regional differences in the locations where S. molle grows showed both qualitative and quantitative differences in essential oil components. This difference was also detected in the biological activities of the essential oils. The anti‐inflammatory capacities of the three samples were assessed using Human Red Blood Cell (HRBC) membrane stabilisation and egg albumin denaturation techniques. The results showed effective anti‐inflammatory activity in all three samples. The antimicrobial efficacy was evaluated using a range of tests, such as anti‐quorum sensing, violacein inhibition, anti‐swimming, and anti‐swarming assays, demonstrating moderate activity. No toxic effects were observed at the tested dosages when assessing cytotoxicity against a healthy cell line (CCD18‐Co). This thorough examination provides valuable insights into the chemical composition and bioactive properties of essential oils derived from S. molle. These findings indicate the possible use of these essential oils in the food, medicinal, and cosmetic industries.
Molecular Profiling of Low‐Grade Appendiceal Mucinous Neoplasms (LAMN)
Authors: Doll Julia , Maurus Katja , Köhler Franziska , Matthes Niels , Lock Johan F. , Germer Christoph‐Thomas , Rosenwald Andreas , Wiegering Armin 
Published: October 2024

ABSTRACTLow‐grade appendiceal mucinous neoplasia (LAMN) represents a relatively rare tumor of the appendix typically diagnosed incidentally through appendectomy for acute appendicitis. In cases where perforation occurs, mucinous content may disseminate into the abdominal cavity, leading to the development of pseudomyxoma peritonei (PMP). The primary objective of this study was to elucidate the molecular characteristics associated with various stages of LAMN and PMP. DNA was extracted from LAMN, primary PMPs, recurrent PMPs, and adenocarcinomas originating from LAMN. The subsequent analysis involved the examination of mutational hotspot regions within 50 cancer‐related genes, covering over 2800 COSMIC mutations, utilizing amplicon‐based next‐generation sequencing (NGS). Our findings revealed activating somatic mutations within the MAPK‐signaling pathway across all tumors examined. Specifically, 98.1% of cases showed mutations in KRAS, while one tumor harbored a BRAF mutation. Additionally, GNAS mutations were identified in 55.8% of tumors, with no significant difference observed between LAMN and PMP. While LAMN rarely displayed additional mutations, 42% of primary PMPs and 60% of recurrent PMPs showed additional mutations. Notably, both adenocarcinomas originating from LAMN showed mutations within TP53. Furthermore, 7.7% (4/52) of cases exhibited a potentially targetable KRAS G12C mutation. In four patients, NGS analysis was performed on both primary PMP and recurrent PMP/adenocarcinoma samples. While mutations in KRAS and GNAS were detected in almost all samples, 50% of recurrent cases displayed an additional SMAD4 mutation, suggesting a notable alteration during disease progression. Our findings indicate two key points: First, mutations within the MAPK pathway, particularly in KRAS, are evident across all tumors, along with a high frequency of GNAS mutations. Second, progression toward PMP or adenocarcinoma is associated with an accumulation of additional mutations within common oncogenic pathways.
Front Cover
Authors: Shah Jatin P. , Levyn Helena , Valero Cristina , Adilbay Dauren , Eagan Alana , Zheng Junting , Gonen Mithat , Cohen Marc , Patel Snehal , Ganly Ian , Pai Prathamesh , Castelnuovo Paolo , Gao Fang Ju , Piazza Cesare , Nicolai Piero , Panizza Ben , Bowman James , Barnett Catherine , Kowalski Luiz P. , Toledo Ronaldo , Fliss Dan M. , DeAlmeida John , Witterick Ian , Herman Philippe , Fontanella Walter , Aniceto Gregorio Sanchez , Hosal Sefik , Ozer Serdar , Iyer Subramania , Harvey Richard , Leemans C. Rene , Hendrickx Jan‐Jaap , Figari Marcelo , Boccalatte Luis , Nibu Ken Ichi , Clarke Peter , Rennie Catherine , Ming Zhu Yi , Cernea Claudio , Goncalves Sergio , Schlosser Rodney , Dias Fernando , Sargi Zoukaa , Ahmed Shahzada , Golusinski Wojciech , Kim Se Heon , Su Shirley Y. , Raza Shaan M. , DeMonte Franco , Hanna Ehab 
Published: October 2024

Front Cover: The cover image is based on the article Skull base surgery for malignant tumors: The 2nd international collaborative study (1995–2015) by Jatin P. Shah MD et al., https://doi.org/10.1002/hed.27746.image
Neuropsychiatric symptoms following the consumption of Lanmaoa asiatica, a poisonous mushroom native to Yunnan
Authors: Dai Ruanxian , Duan Zhantao , Yang Jing , Ning Deyuan , Liu Yu , Gong Bing , Meng Qiang 
Published: October 2024

AbstractObjectivesTo describe and analyze the clinical characteristics and outcomes of Lanmaoa asiatica poisoning cases.MethodsWe conducted a retrospective study of Lanmaoa asiatica poisoning cases admitted to the emergency department of the First People's Hospital of Yunnan Province from January 2023 to December 2023.ResultsA total of 81 patients were admitted, with ages ranging from 14 to 84 years and a median age of 42.5 ± 14.6, predominantly female (72.8%). The peak poisoning cases were observed in July (n = 55, 67.9%). The commonest clinical presentation was the mixed neuropsychiatric and gastroenteritic type (64.2%), while 24.7% of patients presented with neuropsychiatric symptoms only and 11.1% of patients presented with pure gastroenteritic symptoms. The most common first symptom was visual hallucination (n = 56, 69.1%), followed by gastrointestinal symptoms such as nausea (n = 44, 54.3%) and vomiting (n = 36, 44.4%). Around 69.4% of patients had delayed onset of neuropsychiatric symptoms of 12–24 h after mushroom intake and 78.7% of patients had onset of gastroenteritic symptoms within 6 h after ingestion. Laboratory tests showed normal blood routine, liver function, kidney function, cardiac enzymes, and coagulation function. The median length of stay was 3 days, and no fatalities were reported.ConclusionsLanmaoa asiatica poisoning peaked in July. The commonest clinical presentation was the mixed neuropsychiatric and gastroenteritic type. The most common first symptom was visual hallucination. The incubation period for gastrointestinal symptoms was shorter than neuropsychiatric symptoms. Short hospitalization and absence of organ function damage was observed in Lanmaoa asiatica poisoning.
Comparative sequencing study of mismatch repair and homology‐directed repair genes in endometrial cancer and breast cancer patients from Kazakhstan
Authors: Zheng Ying , Vdovichenko Natalia , Schürmann Peter , Ramachandran Dhanya , Geffers Robert , Speith Lisa‐Marie , Bogdanova Natalia , Enßen Julia , Dubrowinskaja Natalia , Yugay Tatyana , Yessimsiitova Zura Berkutovna , Turmanov Nurzhan , Hillemanns Peter , Dörk Thilo 
Published: October 2024

AbstractEndometrial cancer has been associated with pathogenic variants in mismatch repair (MMR) genes, especially in the context of the hereditary Lynch Syndrome. More recently, pathogenic variants in genes of homology‐directed repair (HDR) have also been suggested to contribute to a subset of endometrial cancers. In the present hospital‐based study, we investigated the relative distribution of pathogenic MMR or HDR gene variants in a series of 342 endometrial cancer patients from the Oncology Clinic in Almaty, Kazakhstan. In comparison, we also sequenced 178 breast cancer patients from the same population with the same gene panel. Identified variants were classified according to ClinVar, ESM1b, and AlphaMissense prediction tools. We found 10 endometrial cancer patients (2.9%) carrying pathogenic or likely pathogenic variants in MMR genes (7 MSH6, 1 MSH2, 2 MUTYH), while 14 endometrial cancer patients (4.1%) carried pathogenic variants in HDR genes (4 BRCA2, 3 BRCA1, 3 FANCM, 2 SLX4, 1 BARD1, 1 BRIP1). In the breast cancer series, we found 8 carriers (4.5%) of pathogenic or likely pathogenic variants in MMR genes (2 MSH2, 2 MSH6, 4 MUTYH) while 12 patients (6.7%) harbored pathogenic or likely pathogenic HDR gene variants (5 BRCA1, 3 BRCA2, 1 BRIP1, 1 ERRC4, 1 FANCM, 1 SLX4). One patient who developed breast cancer first and endometrial cancer later carried a novel frameshift variant in MSH6. Our results indicate that MMR and HDR gene variants with predicted pathogenicity occur at substantial frequencies in both breast and endometrial cancer patients from the Kazakh population.
Tips for percutaneous nephrolithotomy for transplant kidney stone
Authors: Yagisawa Takafumi , Shimizu Tomokazu , Tachiki Ayane , Ishiyama Yudai , Onohara Tadashi , Iida Shoichi , Ishida Hideki , Takagi Toshio 
Published: October 2024

IntroductionThe management of urinary tract stones, particularly de novo kidney allograft stones, presents unique challenges for kidney transplant recipients because of their prevalence and specific clinical considerations. Here, we describe a case in which percutaneous nephrolithotomy was successfully used to fragment a large kidney allograft stone ≥20 mm in size.Case presentationA 57‐year‐old woman who underwent ureteroureterostomy post simultaneous pancreas–kidney transplantation presented with gross hematuria after 15 years. Computed tomography revealed a 23‐mm stone in the transplanted kidney. Initial attempts at endoscopic combined intrarenal surgery were changed to percutaneous nephrolithotomy because of poor ureter mobility and tortuosity. Stone fragmentation was achieved using pneumatic and ultrasonic lithotripsy. A second procedure using Swiss LithoClast® Trilogy enabled complete stone clearance and ureteral stent placement.ConclusionBy understanding the peculiarities of the percutaneous approach, we demonstrated the safe and effective use of a pneumatic and ultrasonic lithotripter for kidney allograft stone fragmentation.
Diagnostic value of exosomal N‐glycan profiles for microvascular invasion in hepatocellular carcinoma
Authors: Feng Huijuan , Ke Junping , Zheng Liping , Huang Chenjun , Liu Lijuan 
Published: October 2024

AbstractBackgroundMicrovascular invasion (MVI) was a critical high‐risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma (HCC), and there were no reliable non‐invasive pre‐operative diagnostic markers. The exosomal N‐glycan profile was closely related to the invasion and immune escape of HCC. Therefore, this article investigated the expression of N‐glycan profiles in serum exosomes of patients with HCC and its clinical significance for MVI.MethodsSerum samples from 210 patients with HCC were collected and randomly divided into modeling and validation cohorts. The abundances of N‐glycans in serum exosomes with different MVI grades were determined. A diagnostic model for MVI in HCC based on N‐glycosylation was constructed and the diagnostic value was analyzed.ResultsIn the modeling cohort, comparing groups M0 with M2, the area under the receiver operating characteristic (AUC) of the diagnostic model namely SUM (AUCSUM) was 0.861, the sensitivity of SUM was 92.68%, and the specificity of SUM was 79.41%, all of which were higher than the seven individual indexes (containing Peak 1, Peak 6, Peak 9, Peak 10, Peak 12, AFP, and PIVKA‐II). In the comparison between the M1 and M2 groups, the AUCSUM was 0.749, the sensitivity of SUM was 79.07%, and the specificity of SUM was 76.60%, all of which were higher than the seven individual indexes. When comparing the M0 and M1 groups, the AUCSUM was 0.712, the sensitivity of SUM was 88.57%, and the specificity of SUM was 65.00%. The AUCSUM and sensitivity of SUM were higher than the seven individual indexes and the specificity of SUM was slightly lower than that of AFP (68.18%) but higher than other individual indexes. The results of the validation cohort were similar to those of the modeling cohort.ConclusionThe SUM model of serum exosomes can serve as an auxiliary diagnostic index for MVI staging in patients with HCC.
Unveiling the longitudinal reciprocal relationship between burnout and engagement among adolescent athletes in sport schools
Authors: Kuokkanen Joni , Saarinen Milla , Phipps Daniel J. , Korhonen Johan , Romar Jan‐Erik 
Published: October 2024

AbstractIntroductionBurnout and engagement are pivotal for adolescents' well‐being and have received extensive attention in the educational literature. However, less is known about how these factors develop and interact within and between school and sport when adolescent athletes follow dual (school and sport) careers. The aim of this study was to examine the reciprocal relationship between engagement and burnout in school and sport through a cross‐lagged analysis of longitudinal associations.MethodsA sample of 165 Finnish adolescent athletes (aged 14.5 years, 48.9% girls) enrolled in lower secondary sport schools (i.e., junior high schools) participated in a survey in spring 2019 (i.e., in Grade 8) and 1 year later, in spring 2020 (i.e., in Grade 9).ResultsEngagement and burnout had significant autoregressive relationships within school and sport. Furthermore, low levels of sport engagement in Grade 8 predicted sport burnout in Grade 9, and low levels of school burnout in Grade 8 predicted school engagement in Grade 9. Regarding cross‐domain effects, high levels of school engagement in Grade 8 predicted low levels of sport engagement in Grade 9.ConclusionsThe study shows that school and sport are distinct domains with unique associations between engagement and burnout in each domain. It also shows that adolescent athletes may maintain high school engagement by reducing their sport engagement. These findings highlight the need for sport school practitioners to develop intervention programs to address adolescents' school and sport needs and to support their holistic well‐being.
Swellable Biopolymer Composite Microneedle Patch for Pain‐Free Collection of Interstitial Fluid to Analyze Multiple Biomolecules
Authors: Chauhan Shreya Shashank , Prasad Aditi , Venuganti Venkata Vamsi Krishna 
Published: October 2024

ABSTRACTSampling of interstitial fluid (ISF) using microneedle (MN) patch offers a pain‐free minimally invasive alternative to syringe needle‐based blood sample collection. However, there is a challenge in the development of MN patch that provides swelling behavior with sufficient mechanical strength for skin penetration. Here, we report fabrication of MN patch made of biopolymer composite containing iota‐carrageenan, gelatin, and polyethylene glycol. Calcium chloride was used as a crosslinker to improve mechanical strength. MN patch was characterized for integrity, swelling behavior, mechanical strength, aspiration of fluid from agarose gel, and the excised porcine ear skin. An array of 361 MNs was able to aspirate 36 ± 5 and 14 ± 1 μL fluid after application in agarose gel matrix and the ex vivo porcine skin model, respectively. MN patch applied in vivo rat model for 30 min resulted in the collection of ISF containing 267 ± 128 mg/dL, 24 ± 13 mg/dL, and 0.6 ± 0.4 mIU/mL of glucose, uric acid and thyroid stimulating hormone (TSH), respectively. The concentration of glucose, uric acid, and TSH in rat blood was found to be 199 ± 47 mg/dL, 8.4 ± 6 mg/dL, and 1.1 ± 0.6 mIU/mL at the same time. Furthermore, MN patch applied on the forearm of 10 healthy human volunteers for 30 min was able to aspirate 32 ± 14 μL of ISF. The concentration of glucose, uric acid, and TSH determined from ISF samples of human volunteers was 64 ± 25 mg/dL, 4.2 ± 4.1 mg/dL, and 0.16 ± 0.08 mIU/mL, respectively. The visual analogue scale (VAS) pain score after MN application was lower compared with hypodermic syringe needle insertion. Taken together, biopolymer composite‐based swellable MN patch can be developed for collection of ISF for simultaneous determination of multiple biomolecules in a minimally invasive manner.
The Bacterial Species Behind the Wound and Their Antibacterial Resistant Pattern: A Three‐Year Retrospective Study at St. Dominic Hospital, Akwatia, Ghana
Authors: Deku John Gameli , Aninagyei Enoch , Bedzina Israel , Goloe Francisca Esenam , Eshun Vida Angmorkie , Agyei Eunice , Nmoandor Jonathan Maniye , Duneeh Richard Vikpebah , Duedu Kwabena Obeng 
Published: October 2024

ABSTRACTBackgroundWound infections are often underestimated issues that can lead to chronic illnesses, and since the introduction of antibiotics, wound complications have become less common. However, due to the increased and irrational use of these antibiotics, the resistance in the bacterial isolates has become very common. This has led to reduced treatment options, delay in wound healing, and high treatment costs. This study aimed to investigate bacterial wound infections and their antibiotic resistance at St. Dominic Hospital, Ghana.MethodsA total of 517 records of wound swab culture and susceptibility testing, and patient demographics from 2020 to 2022 were collected from the microbiology unit of St. Dominic Hospital in the Eastern Region of Ghana. The data were entered into Microsoft Excel 2019, cleaned, and exported into IBM SPSS v26 for the statistical analysis. p < 0.05 was considered statistically significant for all analyses.ResultsThe overall prevalence of bacteriological agents causing wound infection in individuals who visited the St. Dominic Hospital from 2020 to 2022 was 70.21% (363/517), with S. aureus 79/363 (21.76%) being the most abundant isolate. Out of the 79 S. aureus isolated, 40 (50.63%) and 39 (49.37%) were resistant to ampicillin and cephalexin, respectively. More than 50% of the predominant Gram‐negative isolate, K. pneumoniae, were resistant to clindamycin 45/72 (62.50%) but susceptible to levofloxacin 70/72 (97.22%), cefotetan 69/72 (95.83%), and chloramphenicol 67/72 (93.06%).ConclusionAntibacterial susceptibility patterns revealed significant resistance trends, particularly among Gram‐negative isolates, emphasizing the urgent need for prudent antibiotic use and ongoing surveillance to combat resistance.
Development and initial validation of the Immigrant Optimism Questionnaire (IOQ) for immigrant‐origin young adults
Authors: Arce Maria Alejandra , Kuperminc Gabriel P. 
Published: October 2024

AbstractImmigrant optimism has been identified as a cultural protective factor that could be targeted in prevention efforts that support immigrant well‐being. However, to date, immigrant optimism has not been consistently operationalized. We report on an iterative and collaborative approach to develop and validate a measure of immigrant optimism. Methods: Content validity was established using feedback from immigrant‐origin young adults and from experts in the field. Then, using survey data from 241 immigrant‐origin young adults, we examined reliability, validity, and invariance of our measure among first‐ and second‐generation immigrants. Results: Confirmatory factor analyses indicated that a single immigrant optimism factor explains significant variation in each of our indicators. Our measure was further invariant across first‐ and second‐generation immigrant youth and demonstrated convergent and discriminant validity. Conclusions: Findings support the use of our measure with immigrant‐origin young adults. We offer considerations for future research and highlight implications for prevention efforts.
Low fluid shear stress stimulates the uptake of noxious endothelial extracellular vesicles via MCAM and PECAM‐1 cell adhesion molecules
Authors: Coly Pierre‐Michaël , Chatterjee Shruti , Mezine Fariza , Jekmek Christelle El , Devue Cécile , Nipoti Thomas , Mazlan Stephane , Corona Maribel Lara , Dingli Florent , Loew Damarys , van Niel Guillaume , Loyer Xavier , Boulanger Chantal M. 
Published: October 2024

AbstractAtherosclerotic lesions mainly form in arterial areas exposed to low shear stress (LSS), where endothelial cells express a senescent and inflammatory phenotype. Conversely, areas exposed to high shear stress (HSS) are protected from plaque development. Endothelial extracellular vesicles (EVs) have been shown to regulate inflammation and senescence, and therefore play a crucial role in vascular homeostasis. Whilst previous studies have shown links between hemodynamic forces and EV release, the effects of shear stress on the release and uptake of endothelial EVs remains elusive. We aim to decipher the interplay between these processes in endothelial cells exposed to atheroprone or atheroprotective shear stress. Confluent HUVECs were exposed to LSS or HSS for 24 h. Large and small EVs were isolated from conditioned medium by centrifugation and size exclusion chromatography. They were characterised by TEM, Western blot, tunable resistive pulse sensing, flow cytometry and proteomics. Uptake experiments were performed using fluorescently‐labelled EVs and differences between groups were assessed by flow cytometry and confocal microscopy. We found that levels of large and small EVs in conditioned media were fifty and five times higher in HSS than in LSS conditions, respectively. In vivo and in vitro uptake experiments revealed greater EV incorporation by cells exposed to LSS conditions. Additionally, endothelial LSS‐EVs have a greater affinity for HUVECs than HSS‐EVs or EVs derived from platelets, erythrocytes and leukocytes. Proteomic analysis revealed that LSS‐EVs were enriched in adhesion proteins (PECAM1, MCAM), participating in EV uptake by endothelial cells. LSS‐EVs also carried mitochondrial material, which may be implicated in elevating ROS levels in recipient cells. These findings suggest that shear stress influences EV biogenesis and uptake. Given the major role of EVs and shear stress in vascular health, deciphering the relation between these processes may yield innovative strategies for the early detection and treatment of endothelial dysfunction.