bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thrombin generation correlates with disease duration in multiple sclerosis (MS): Novel insights into the MS-associated prothrombotic state
Authors: Parsons Martin EM , O’Connell Karen , Allen Seamus , Egan Karl , Szklanna Paulina B , McGuigan Christopher , Ní Áinle Fionnuala , Maguire Patricia B 
Published: December 2017

Background Thrombin is well recognised for its role in the coagulation cascade but it also plays a role in inflammation, with enhanced thrombin generation observed in several inflammatory disorders. Although patients with multiple sclerosis (MS) have a higher incidence of thrombotic disease, thrombin generation has not been studied to date. Objectives The aim of this study was to characterise calibrated automated thrombography parameters in patients with relapsing–remitting MS (RRMS) and primary progressive MS (PPMS) in comparison to healthy controls (HCs). Methods Calibrated automated thrombography was performed on platelet poor plasma from 15 patients with RRMS, 15 with PPMS and 19 HCs. Results We found that patients with RRMS generate thrombin at a significantly faster rate than the less inflammatory subtype, PPMS or HCs. In addition, the speed of thrombin generation was significantly correlated with time from clinical diagnosis in both subtypes. However, in RRMS the rate of thrombin generation was increased with increased time from clinical diagnosis, while in PPMS the rate of thrombin generation decreased with increased time from clinical diagnosis. Conclusions These data likely reflect the differential active proinflammatory states in each MS subtype and provide novel mechanistic insights into the clinically relevant prothrombotic state observed in these patients.
Pulmonary edema following central nervous system lesions induced by a non- mouse-adapted EV71 strain in neonatal BALB/c mice
Authors: Jin Yuefei , Zhang Chao , Zhang Rongguang , Ren Jingchao , Chen Shuaiyin , Sui Meili , Zhou Guangyuan , Dang Dejian , Zhu Jiehui , Feng Huifen , Xi Yuanlin , Yang Haiyan , Duan Guangcai 
Published: December 2017

Abstract
Background
Enterovirus (EV) infection has been a serious health issue in Asia-Pacific region. It has been indicated that the occurrence of fatal hand foot and mouth disease (HFMD) cases following EV71 infection is mainly attributed to pulmonary edema. However, the development of pulmonary disorders after EV71 infection remains largely unknown. To establish an EV71-infected animal model and further explore the underlying association of central nervous system (CNS) invasion with pulmonary edema, we isolated a clinical source EV71 strain (ZZ1350) from a severe case in Henan Province.

Methods
We evaluated the cytotoxicity of ZZ1350 strain and the susceptibility in 3-day-old BALB/c mice with intraperitoneal, intracerebral and intramuscular inoculation. Various histopathological and immunohistochemical techniques were applied to determine the target organs or tissue damage after infection. Correlation analysis was used to identify the relationship between CNS injury and pulmonary disorders.

Results
Our experimental results suggested that ZZ1350 (C4 subtype) had high cytotoxicity against African green monkey kidney (Vero) cells and human rhabdomyosarcoma (RD) cells and neonatal BALB/c mice were highly susceptible to the infection with ZZ1350 through three different inoculation routes (2 × 106 pfu/mouse) exhibiting severe neurological and respiratory symptoms that were similar to clinical observation. Viral replication was found in brain, spinal cord, skeletal muscle, lung, spleen, liver, heart of infected mice and these sections also showed histopathological changes. We found that brain histology score was positive correlated with lung histology score in total experimental mice and mice under the three inoculation routes (P < 0.05). At the same time, there were positive correlations between spinal cord score and lung score in total experimental mice and mice with intracerebral inoculation (P < 0.05).

Conclusions
ZZ1350 strain is effective to establish animal model of EV71 infection with severe neurological and respiratory symptoms. The development of pulmonary disorders after EV71 infection is associated with severity of CNS damage.
Using incentives to recruit physicians into behavioral trials: lessons learned from four studies
Authors: Mohan Deepika , Rosengart Matthew R. , Fischhoff Baruch , Angus Derek C. , Wallace David J. , Farris Coreen , Yealy Donald M. , Barnato Amber E. 
Published: December 2017

Abstract
Objective
To describe lessons learned from the use of different strategies for recruiting physicians responsible for trauma triage, we summarize recruitment data from four behavioral trials run in the United States between 2010 and 2016.

Results
We ran a series of behavioral trials with the primary objective of understanding the influence of heuristics on physician decision making in trauma triage. Three studies were observational; one tested an intervention. The trials used different methods of recruitment (in-person vs. email), timing of the honorarium (pre-paid vs. conditional on completion), type of honorarium [a $100 gift card (monetary reward) vs. an iPad mini 2 (material incentive)], and study tasks (a vignette-based questionnaire, virtual simulation, and intervention plus virtual simulation). We recruited 989 physicians, asking each to complete a questionnaire or virtual simulation online. Recruitment and response rates were 80% in the study where we approached physicians in person, used a pre-paid material incentive, and required that they complete both an intervention plus a virtual simulation. They were 56% when we recruited physicians via email, used a monetary incentive conditional on completion of the task, and required that they complete a vignette-based questionnaire.
Trial registration clinicaltrials.gov; NCT02857348
CRISPR/Cas9‒Mediated Tspo Gene Mutations Lead to Reduced Mitochondrial Membrane Potential and Steroid Formation in MA-10 Mouse Tumor Leydig Cells
Authors: Fan Jinjiang , Wang Kevin , Zirkin Barry , Papadopoulos Vassilios 
Published: December 2017

AbstractThe outer mitochondrial membrane translocator protein (TSPO) binds cholesterol with high affinity and is involved in mediating its delivery into mitochondria, the rate-limiting step in hormone-induced steroidogenesis. Specific ligand binding to TSPO has been shown to initiate steroid formation. However, recent studies of the genetic deletion of Tspo have provided conflicting results. Here, we address and extend previous studies by examining the effects of Tspo-specific mutations on steroid formation in hormone- and cyclic adenosine monophosphate (cAMP)–responsive MA-10 cells, using the CRISPR/Cas9 system. Two mutant subcell lines, nG1 and G2G, each carrying a Tspo exon2-specific genome modification, and two control subcell lines, G1 and HH, each carrying a wild-type Tspo, were produced. In response to dibutyryl cAMP, the nG1 and G2G cells produced progesterone at levels significantly lower than those produced by the corresponding control cells G1 and HH. Neutral lipid homeostasis, which provides free cholesterol for steroid biosynthesis, was altered significantly in the Tspo mutant cells. Interestingly, the mitochondrial membrane potential (ΔΨm) of the Tspo mutant cells was significantly reduced compared with that of the control cells, likely because of TSPO interactions with the voltage-dependent anion channel and tubulin at the outer mitochondrial membrane. Steroidogenic acute regulatory protein (STAR) expression was induced in nG1 cells, suggesting that reduced TSPO affected STAR synthesis and/or processing. Taken together, these results provide further evidence for the critical role of TSPO in steroid biosynthesis and suggest that it may function at least in part via its regulation of ΔΨm and effects on STAR.
Evidence Supporting a Role for Constitutive Ghrelin Receptor Signaling in Fasting-Induced Hyperphagia in Male Mice
Authors: Fernandez Gimena , Cabral Agustina , Andreoli María F , Labarthe Alexandra , M'Kadmi Céline , Ramos Jorge G , Marie Jacky , Fehrentz Jean-Alain , Epelbaum Jacques , Tolle Virginie , Perello Mario 
Published: December 2017

AbstractGhrelin is a potent orexigenic peptide hormone that acts through the growth hormone secretagogue receptor (GHSR), a G protein–coupled receptor highly expressed in the hypothalamus. In vitro studies have shown that GHSR displays a high constitutive activity, whose physiological relevance is uncertain. As GHSR gene expression in the hypothalamus is known to increase in fasting conditions, we tested the hypothesis that constitutive GHSR activity at the hypothalamic level drives the fasting-induced hyperphagia. We found that refed wild-type (WT) mice displayed a robust hyperphagia that continued for 5 days after refeeding and changed their food intake daily pattern. Fasted WT mice showed an increase in plasma ghrelin levels, as well as in GHSR expression levels and ghrelin binding sites in the hypothalamic arcuate nucleus. When fasting-refeeding responses were evaluated in ghrelin- or GHSR-deficient mice, only the latter displayed an ∼15% smaller hyperphagia, compared with WT mice. Finally, fasting-induced hyperphagia of WT mice was significantly smaller in mice centrally treated with the GHSR inverse agonist K-(D-1-Nal)-FwLL-NH2, compared with mice treated with vehicle, whereas it was unaffected in mice centrally treated with the GHSR antagonists D-Lys3-growth hormone–releasing peptide 6 or JMV2959. Taken together, genetic models and pharmacological results support the notion that constitutive GHSR activity modulates the magnitude of the compensatory hyperphagia triggered by fasting. Thus, the hypothalamic GHSR signaling system could affect the set point of daily food intake, independently of plasma ghrelin levels, in situations of negative energy balance.
A proposal to change the name of the NBPF/DUF1220 domain to the Olduvai domain
Authors: Sikela James M. , van Roy Frans 
Published: December 2017

We are jointly proposing a new name for a protein domain of approximately 65 amino acids that has been previously termed NBPF or DUF1220. Our two labs independently reported the initial studies of this domain, which is encoded almost entirely within a single gene family. The name Neuroblastoma Breakpoint Family (NBPF) was applied to this gene family when the first identified member of the family was found to be interrupted in an individual with neuroblastoma. Prior to this discovery, the PFAM database had termed the domain DUF1220, denoting it as one of many protein domains of unknown function. It has been PFAM’s intention to use “DUF” nomenclature to serve only as a temporary placeholder until more appropriate names are proposed based on research findings. We believe that additional studies of this domain, primarily from our laboratories over the past 10 years, have resulted in furthering our understanding of these sequences to the point where proposing a new name for this domain is warranted. Because of considerable data linking the domain to human-specific evolution, brain expansion and cognition, we believe a name reflecting these findings would be appropriate. With this in mind, we have chosen to name the domain (and the repeat that encodes it) Olduvai. The gene family will remain as NBPF for now. The primary domain subtypes will retain their previously assigned names (e.g. CON1-3; HLS1-3), and the three-domain block that expanded dramatically in the human lineage will be termed the Olduvai triplet. The new name refers to Olduvai Gorge, which is a site in East Africa that has been the source of major anthropological discoveries in the early-mid 1900’s. We also chose the name as a tribute to the scientists who made important contributions to the early studies of human origins and our African genesis.
Elements of place and nation branding in Ocho apellidos vascos (2014)
Authors: Martínez-Expósito Alfredo 
Published: December 2017

Abstract
This article considers the ways in which Ocho apellidos vascos (2014) mobilizes salient aspects of nation branding, such as communication strategies that privilege a tourist’s gaze, authentication of local stereotypes, selective recycling of history, the commodification of place and commercial nationalism. The film was produced and released in 2014, only three years after ETA’s historic cease-fire declaration. The national context for the release was marked by the end of terrorist violence in the Basque Country, growing separatist sentiment in Catalonia and the concomitant decline in popularity of Brand Spain. Ocho apellidos vascos invokes branding discourses that have impact well beyond the seemingly innocuous regional stereotypes that structure the film’s imagery and plot. This article explores the influence of these discourses on the film’s branding of three key ‘places’: Spain, Seville and the Basque Country. In particular, it discusses the process by which ‘Spain’ is subject to erasure, ‘Seville’ is branded as a synecdoche for the whole of Spain and ‘the Basque Country’ is subject to an othering gaze that is incongruous with the recently formulated Basque Brand.
Basil Fawlty as a ‘pre-Thatcherite’ conservative in Fawlty Towers
Authors: Mähkä Rami 
Published: December 2017

Abstract
The article analyses the popular British situation comedy Fawlty Towers (1975, 1979) as a comedic representation of conservatism and its lead character, Basil Fawlty (played by John Cleese), as a ‘pre-Thatcherite’. The article discusses how the character of Fawlty can help us understand the processes that led to Margaret Thatcher winning the election in 1979 and becoming prime minister. Fawlty promotes similar values to Thatcher, but with ambiguity as the series is a comedy; comedy makes interpretation of ideological issues problematic as it is an alternative meaning-making system to non-comedic forms.
Polluting young minds? Smash Hits and ‘high Thatcherism’
Authors: Kirkham Neil 
Published: December 2017

Abstract
This article is an analysis of the British popular music publication Smash Hits at the peak of its commercial power in the late 1980s. Whereas academic attention has been diverted towards its early, more ‘alternative’ period in the late 1970s, as Railton suggests, like many similar publications it has not been widely studied within the academy and particularly not in relation to its engagement with overtly popular music. On the rare occasions it is discussed it is broadly dismissed as being conservative and overtly consumerist, merely a ‘teen glossy’ that provided posters for teenage girls’ bedrooms. Through a content analysis of issues published in 1987, with a specific focus on an interview with Margaret Thatcher conducted by Smash Hits in March of that year, this article will attempt to redress this imbalance. It aims to demonstrate that as a guide to the politics of popular music and youth culture at the end of ‘high Thatcherism’, Smash Hits deserves to be studied with the same respect as it’s more credible ‘inkie’ and ‘indie’ peers of the period. It will also suggest that the very nature of Smash Hits as a reflection of populist taste allowed it the scope to cover, as cultural texts, a range of musical performers and styles far broader than that seen in the indie press of the time and that, in turn, this allowed it to visualize key youth movements they ignored. Finally, it also hopes to outline how it’s representation of gender, sexuality and race was very much at odds with mainstream, and specifically Thatcherite, ideals in this period.
Where there is discord, may we bring harmony: Government economic policy and independent record labels in the United Kingdom in the 1980s
Authors: Baillie Mark 
Published: December 2017

Abstract
This article seeks to examine the relationship between independent popular music cultural production in the United Kingdom in the 1980s and the policies and political philosophy of Thatcherism. Although often regarded as being oppositional forces, closer scrutiny reveals areas of significant complementarity, most notably in the influence of the Enterprise Allowance Scheme on various emerging independent record labels. The first part of the article will look at genuine attempts at resistance to Thatcherism in popular music culture such as Red Wedge, and cite various ‘common-sense’ ideas around anti-Thatcherism in contemporary popular music culture. The second part looks at the historical roots of Thatcherism in order to provide a definitional framework as well as to explore some of the ambiguities around Thatcherism itself (particularly regarding the competing strands of social conservatism and economic liberalism) and the central importance of the promotion of an ‘enterprise culture’ based on self-reliance and entrepreneurship. An examination of the role played by the Enterprise Allowance Scheme in the development of independent record labels follows in order to assess the degree to which the economic liberalism of Thatcherism found an expression in independent popular music culture.
DESIDOC Journal of Library and Information Technology (DJLIT): A Bibliometric Analysis of Cited References
Authors: Bapte Vishal Dattatray 
Published: December 2017

<p>The paper presents a bibliometric analysis of the 4821 cited documents appended to the 295 articles published in DJLIT during 2011-15. The citation analysis is based on various strictures such as studying distribution of citations, authorship pattern, degree of collaboration, distribution of reference sources, prominent authors and ranked list of core journals. The study revealed that there is dominance of single authorship with 1912 (39.65%) citations followed by two authors with 1152 (23.89%) citations, three authors with 456 (9.45%) citations and more than three authors with 386 (8%) citations. There has been the availability of a good degree of institutional publications as well. Year-wise authorship pattern is also specified so as to see the dominance of particular authorship pattern for the period considered under the study. The degree of authors’ collaboration for the present study is 0.51 and modified collaborative coefficient is 0.3661. Dr B.M. Gupta with 52 citations is the most prolific author. Dr K.C. Garg and Dr B.S. Kademani are at the second and third position respectively. The study further exposed the journal to be the mostly cited information source 2560 (53.10%) followed by websites (22.69%) and books (10.81%). Conference papers, reports, theses, workshop papers and seminar papers equally seem to be preferred domain with regard to using pertinent information source. Ranked list of journals denotes Scientometrics to be the most used journal (6.60%) by the authors contributing in DJLIT. The source journal is at the second position in the ranked list with 5.43%. A glance at the ranked core list of journals suggests that maximum journals are from foreign countries.</p>
Separation Dynamics of Air-to-Air Missile and Validation with Flight Data
Authors: Chakraborty Debasis , Anandhanarayanan K , Raj Ankit , Shah Vaibhav , Krishnamurthy R 
Published: December 2017

<p class="p1">Prediction of flight characteristics of a store in the vicinity of an aircraft is vitally important for ensuring the safety of the aircraft and effectiveness of the store to meet the mission objective. Separation dynamics of an agile air-to-air-Missile from a fighter aircraft is numerically simulated using an integrated store separation dynamics suite. Chimera cloud of points along with a grid-free Euler solver is used to obtain aerodynamic force on the missile and the force is integrated using a rigid body dynamics code to obtain the missile position. In the present work, the suite is applied to a flight test case and sensitivity of trajectory variables on launch parameters is studied. Further, the results of the suite are compared with the flight data. The predicted body rates and Euler angles of missile compare well with the flight data.<span class="Apple-converted-space"> </span></p><p class="p2"><span class="Apple-converted-space"> </span></p>
Design and Development of Redeployable Underwater Data Communication Link for Defence Application
Authors: Kumar Arun , Anjaneyulu L. 
Published: December 2017

<p class="p1">Testing of underwater system for defence application is carried out from submersible platform at specified depth. The underwater platform houses the article under test, equipment and instruments required to conduct the test and to control the platform. During development phase of defence systems, large numbers of sensors are mounted on the test article and data is collected to validate the design. Data acquisition system, video camera and high speed video cameras are positioned on the platform to record physical parameters and observe visually the performance of the article under evaluation. Since the data and video signals are parallely recorded on ship, the bandwidth demand for real-time data communication from underwater platform to control station is very high. The existing technology presently used for underwater communication has limitation of bandwidth and not suitable for defence application. This paper describes in detail the design and development of a re-deployable data communication link by laying a specially designed negatively buoyant fibre optic cable in high-sea from ship to underwater platform to provide higher bandwidth required for defence application. The link has been successfully used for sea state less than one during evaluation of underwater defence system. The availability of bandwidth from underwater platform to control system can be increased significantly by laying fibre optic cable in high sea. It also provides unlimited bandwidth for the above requirement.<span class="Apple-converted-space"> </span></p>
Skills Development in The Asia-Pacific Maritime World: A Comparative Study of Vocational Education in Malaysia and Indonesia
Authors: Mustapha Ramlee 
Published: December 2017

This region of Southeast Asia shares more social and cultural ties with other Austronesian peoples in the Pacific than with the peoples of mainland Southeast Asia. Contemporarily, Asia Pacific is still the fastest growing economic region in the world despite economic turbulence and uncertainties in recent years due to the global economic slow-down. As one of centres of economic power, the region could hardly remain immune to the globalizing impact of economic and technological change. The purpose of this study was to explore the development of Technical and Vocational education in Malaysia and Indonesia by analyzing the history, policies, and its direction. In Malaya, the Technical and Vocational education prior to independence had projected the images of “colonial apprenticeship” with the emphasis on manual agricultural and crafts, which aimed at training the Malay students to fill positions in the Railway department under the Federated Malay States. After independence, Technical and Vocational education in Malaysia continued to grow, and some reforms have been implemented to improve the image of Technical and Vocational education itself. In Indonesia, a similar development occurred but the difference is in terms of Technical and Vocational education funding at the secondary level where there are many private Technical and Vocational schools as compared to Malaysia. There are new concepts introduced in Technical and Vocational education, such as the Tech-Prep, Time Sector Privatization and Vocational Colleges in Malaysia and Link and Match, Dual System, Product-based Curriculum, and Total Performance Management (TPM) in Indonesia, but the concepts are yet to be carried out optimally due of some constraints. The implications of this study are to identify the human capital development in the maritime Archipelago countries from the perspectives of the competitiveness and the preparation for dealing with the impact of globalization.