bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a series of silk fibroin protein‐based nonwoven mats for use as an anti‐adhesion patch for wound management in robotic surgery
Authors: Wang Yu‐Chi , Bai Meng‐Yi , Hsu Wan‐Yuan , Yu Mu‐Hsien 
Published: September 2017

AbstractA novel anti‐adhesion nonwoven mat mainly composed of silk fibroin protein (SFP) was fabricated via the single‐spinneret electrospinning technique. A series of SFP‐based electrospun nonwoven mats containing additives of different synthetic polymer ratios, such as pure SFP, SFP/poly(vinyl alcohol) (PVA), SFP/polyethylene glycol (PEG), and SFP/polyethylene oxide (PEO) were produced and compared. All membranes were porous and had diameters of 324.02 ± 113.7, 308.86 ± 74.02, 366.22 ± 115.81, and 341.82 ± 119.42 nm, respectively. The average pore size for each membrane was 1.132 ± 0.99, 0.811 ± 0.424, 0.975 ± 0.741, and 0.784 ± 0.497 μm2. No nonwoven mats showed significant cytotoxicity toward fibroblast cells based on the results of MTT assays. Surprisingly, for all groups of SFP‐based nonwoven mats, nitrate formation was reduced by up to 94.55 ± 14.50%, 92.16 ± 19.38%, 91.28 ± 28.375%, and 92.00 ± 12.64% in lipopolysaccharide‐induced RAW 264.7 macrophages model. Tissue anti‐adhesion potential was evaluated in an in vitro fibroblast cell adhesion model and in vivo wounded mice model. In vitro, the mean cell anti‐adhesion percentage of fibroblast cells changed over time in the following order: PVA/SFP > SFP > PEG/SFP∼PEO/SFP. In vivo, SFP and PVA/SFP‐treated groups both showed superior collagen regeneration and wound closure. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 221–230, 2018.
Extracellular matrix derived from periodontal ligament cells maintains their stemness and enhances redifferentiation via the wnt pathway
Authors: Zhang Ji‐Chun , Song Zhong‐Chen , Xia Yi‐Ru , Shu Rong 
Published: September 2017

AbstractLarge numbers of viable cells cannot be obtained from periodontal ligament tissues of patients with periodontitis. Therefore, it is imperative to establish an ex vivo environment that can support cell proliferation and delay senescence. Here, we have successfully reconstructed a native extracellular matrix (ECM), derived from early‐passage human periodontal ligament cells (PDLCs) using the NH4OH/Triton X‐100 protocol. The ECM was investigated by scanning electron microscopy and immunostaining for specific ECM proteins (collagen I and fibronectin). Late‐passage ECM‐expanded PDLCs exhibited a much higher proliferation index and lower levels of reactive oxygen species (ROS), confirmed by the increased expression of pluripotent markers and enhanced osteogenic capacity. Interestingly, the Wnt pathway was suppressed during the ECM expansion‐mediated increase in pluripotency, but was activated in an osteogenic differentiation environment, as confirmed by treatment with the XAV‐939 β‐catenin inhibitor or the SP600125 c‐Jun N‐terminal kinase (JNK) inhibitor. Cell sheets formed by ECM‐expanded PDLCs exhibited an enhanced periodontal tissue regeneration capacity compared to those formed on tissue culture polystyrene (TCP) surfaces in vivo. Taken together, the cell‐free ECM provides a tissue‐specific cell niche for the ex vivo expansion of PDLCs while retaining stemness and osteogenic potential, partially via the Wnt pathway. This represents a promising matrix for future applications in periodontal tissue regeneration therapy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 272–284, 2018.
Retracted: Effects of Glut1 gene silencing on proliferation, differentiation, and apoptosis of colorectal cancer cells by targeting the TGF‐β/PI3K‐AKT‐mTOR signaling pathway
Authors: Wu Xue‐Liang , Wang Li‐Kun , Yang Dong‐Dong , Qu Ming , Yang Yong‐Jiang , Guo Fei , Han Lei , Xue Jun 
Published: September 2017

AbstractThis study aims to investigate the effects of glucose transport l (Glut1) gene on proliferation, differentiation, and apoptosis of colorectal cancer (CRC) cells by regulating the TGF‐β/PI3K‐AKT‐mTOR signaling pathway. Immunohistochemistry was conducted to detect the positive Glut1 expression. Normal human CRC epithelial cells (CCD‐18Co) and CRC cell line HCT116 were grouped into the control, blank, negative control (NC), and shGlut1‐1 groups. RT‐qPCR and Western blotting were performed to detect the expressions of Glut1, TGF‐β1, PI3K, AKT, PTEN, mTOR, Bcl‐2, and Bax. Protein expression of phosphorylated‐PI3K (p‐PI3K), p‐S473‐AKT, p‐S389‐S6K1, p‐T70‐4EBP1, Cleaved caspase‐3 and Cleaved‐PARP were detected. MTT assay, flow cytometry, and colony formation assay were performed in order to detect cell viability, cell cycle, and apoptosis, respectively. The positive expression rate of Glut1 in CRC tissues was 75% ± 8%, while in the adjacent normal tissues it was 0%. In comparison to adjacent normal tissues, CRC tissues had increased Glut1, TGF‐β1, PI3K, AKT, mTOR, and Bcl‐2 expressions, and p‐PI3K, p‐S473‐AKT, p‐S389‐S6K1, and p‐T70‐4EBP1 expressions; and decreased PTEN, Bax, Cleaved caspase‐3, and Cleaved‐PARP expressions. In comparison with the blank and NC groups, cells in the shGlut1‐1 group showed decreased Glut1, TGF‐β1, PI3K, AKT, mTOR, and Bcl‐2 expressions, and p‐PI3K, p‐S473‐AKT, p‐S389‐S6K1, and p‐T70‐4EBP1 expressions; and increased PTEN, Bax, Cleaved caspase‐3, and Cleaved‐PARP expressions, along with more arrested cells in C0/C1 phase than in S phase and slower cell growth. These results suggested that silencing the Glut1 gene inhibited proliferation and promoted apoptosis of CRC cells by inactivating TGF‐β/PI3K‐AKT‐mTOR signaling pathway.
Retracted: Effects of cyclin D1 gene silencing on cell proliferation, cell cycle, and apoptosis of hepatocellular carcinoma cells
Authors: Chen Jin , Li Xue , Cheng Qi , Ning Deng , Ma Jie , Zhang Zhi‐Ping , Chen Xiao‐Ping , Jiang Li 
Published: September 2017

AbstractThis study aims to investigate the effects of Cyclin D1 silencing on cell cycle, cell proliferation, and apoptosis of hepatocellular carcinoma cells (HCC). Cells were divided into the blank group, negative control group (HCC cells transfected with control shRNA), Cyclin D1 shRNA group (HCC cells transfected with Cyclin D1 shRNA), and the normal group (human normal liver L‐02 cells). Expressions of Cyclin D1, Caspase‐3, Bcl‐2, and C‐myc were detected by RT‐qPCR and Western blotting. Cell proliferation was detected by Cell Counting Kit‐8. Cell cycle and apoptosis were detected by flow cytometry. Tumor xenograft in nude mice was performed to detect in vivo tumorigenesis. HCC tissues and HCC cells exhibited elevated expression levels of Cyclin D1. Cyclin D1 expression levels was found to be correlated with tumor size and tumor staging. Compared with the normal group, the blank group showed enhanced cell proliferation, a reduction in the amount of cells in G0/G1 phase, increased number cells in S and G2/M phase, reduced apoptosis, elevated expressions of Cyclin D1, Bcl‐2, and C‐myc, decreased Caspase‐3 activity and significant tumorigenicity. In comparison with the blank group, the Cyclin D1 shRNA group revealed weakened cell proliferation, reduced cells in S and G2/M phase, increased cells in G0/G1 phase, increased Annexin V positive cell ratio, decreased expression of Cyclin D1, Bcl‐2, and C‐myc, elevated Caspase‐3 activity and inhibited tumorigenicity. In conclusion, Cyclin D1 gene silencing suppresses cell proliferation and inhibits cell apoptosis, which may be a new target approach in the treatment and management for HCC.
Targeting intracellularStaphylococcus aureusto lower recurrence of orthopaedic infection
Authors: Dusane Devendra H. , Kyrouac Douglas , Petersen Iris , Bushrow Luke , Calhoun Jason H. , Granger Jeffrey F. , Phieffer Laura S. , Stoodley Paul 
Published: September 2017

ABSTRACTStaphylococcus aureus is often found in orthopaedic infections and may be protected from commonly prescribed antibiotics by forming biofilms or growing intracellularly within osteoblasts. To investigate the effect of non‐antibiotic compounds in conjunction with antibiotics to clear intracellular and biofilm formingS. aureuscausing osteomyelitis. SAOS‐2 osteoblast‐like cell lines were infected withS. aureusBB1279. Antibiotics (vancomycin, VAN; and dicloxacillin, DICLOX), bacterial efflux pump inhibitors (piperine, PIP; carbonyl cyanide m‐chlorophenyl hydrazone, CCCP), and bone morphogenetic protein (BMP‐2) were evaluated individually and in combination to kill intracellular bacteria. We present direct evidence that after gentamicin killed extracellular planktonic bacteria and antibiotics had been stopped, seeding from the infected osteoblasts grew as biofilms. VAN was ineffective in treating the intracellular bacteria even at 10× MIC; however in presence of PIP or CCCP the intracellularS. aureuswas significantly reduced. Bacterial efflux pump inhibitors (PIP and CCCP) were effective in enhancing permeability of antibiotics within the osteoblasts and facilitated killing of intracellularS. aureus. Confocal laser scanning microscopy (CLSM) showed increased uptake of propidium iodide within osteoblasts in presence of PIP and CCCP. BMP‐2 had no effect on growth ofS. aureuseither alone or in combination with antibiotics. Combined application of antibiotics and natural agents could help in the treatment of osteoblast infected intracellular bacteria and biofilms associated with osteomyelitis. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1086–1092, 2018.
Changes in physiological and some nutritional, nutraceuticals, chemical–physical, microbiological and sensory quality of minimally processed cactus pears cvs ‘Bianca’, ‘Gialla’ and ‘Rossa’ stored under passive modified atmosphere
Authors: Palma Amedeo , Continella Alberto , La Malfa Stefano , D'Aquino Salvatore 
Published: September 2017

AbstractBACKGROUNDThe objective of this study was to compare the overall quality changes of minimally processed cactus pears cvs ‘Bianca’, Gialla' and ‘Rossa’ stored at 4 °C for 10 days. Periodically in‐package CO2, O2 and C2H4 were determined and fruit were assessed for overall quality changes (pH, acidity, sugars, phenolics, betacyanins and betaxanthines, antioxidant capacity, colour, firmness, microbiological population and sensory attributes). In a preliminary study three different polymeric films were tested to select the most suitable to design a package with a short lag time to achieve steady‐state conditions.RESULTSResults showed marked differences between measured in‐package CO2 and O2 values and those calculated based on respiration of peeled fruit and film permeance to CO2 and O2 provided by manufactures. The sensory evaluation of packed fruit indicated in film BBT‐Bolphane, which created a steady‐state in‐package partial pressure for CO2 of 4.3–4.8 kPa and for O2 of 4.8–5.5 kPa, as the best film. Results of in‐package gas composition with the three cultivars were similar to those achieved in cv. ‘Gialla’ with the preliminary test. All measured qualitative parameters changed slightly over the storage period for all cultivars and followed the same trend, despite significant differences existing among cultivars.CONCLUSIONThis study clearly showed a similar physiological behavior of minimally processed ‘Bianca’, ‘Gialla’ and ‘Rossa’ cactus pears. Storage conditions optimal for one cultivar fit well for the others; thus mixing fruit of different cultivars in a package designed for one specific cultivar does not lead to relevant deviation from expected results. © 2017 Society of Chemical Industry
Anti‐inflammatory effects of polyphenols in arthritis
Authors: Oliviero Francesca , Scanu Anna , Zamudio‐Cuevas Yessica , Punzi Leonardo , Spinella Paolo 
Published: September 2017

AbstractPolyphenols have been extensively investigated with regard to their antioxidant, anti‐inflammatory, and immunomodulant properties in many inflammatory chronic conditions. The aim of this review is to summarise how these compounds can modulate the inflammatory pathways which characterise the most prevalent arthropathies including osteoarthritis, rheumatoid arthritis and crystal‐induced arthritis. Among polyphenols, epigallocatechin gallate, carnosol, hydroxytyrosol, curcumin, resveratrol, kaempferol and genistein have been the most widely investigated in arthritis. The most important results of the studies outlined in this article show how polyphenolic compounds are able to inhibit the expression and the release of a number of pro‐inflammatory mediators and proteolytic enzymes, the activity of different transcriptional factors and the production of reactive oxygen species in vitro. Studies on animal models of rheumatoid arthritis, osteoarthritis and gout show interesting results in terms of reduced tissue damage, restored cartilage homeostasis, and decreased levels of uric acid, respectively. Despite the multiple protective effects of polyphenols, there are no dietary recommendations for patients affected by rheumatic diseases. Future studies, including intervention trials, should be conducted to determine the relevance of polyphenols consumption or supplementation in arthritis. © 2017 Society of Chemical Industry
Microwave heating modelling of a green smoothie: Effects on glucoraphanin, sulforaphane and S‐methyl cysteine sulfoxide changes during storage
Authors: Castillejo Noelia , Martínez‐Hernández Ginés Benito , Lozano‐Guerrero Antonio José , Pedreño‐Molina Juan Luis , Gómez Perla A , Aguayo Encarna , Artés Francisco , Artés‐Hernández Francisco 
Published: September 2017

AbstractBACKGROUNDThe heating of a green smoothie during an innovative semi‐continuous microwave treatment (MW; 9 kW for 15 s) was modelled. Thermal and dielectric properties of the samples were previously determined. Furthermore, the heating effect on the main chemopreventive compounds of the smoothie and during its subsequent storage up to 30 days at 5 or 15 °C were studied. Such results were compared to conventional pasteurisation (CP; 90 °C for 45 s) while unheated fresh blended samples were used as the control.RESULTSA procedure was developed to predict the temperature distribution in samples inside the MW oven with the help of numerical tools. MW‐treated samples showed the highest sulforaphane formation after 20 days, regardless of the storage temperature, while its content was two‐fold reduced in CP samples. Storage of the smoothie at 5 °C is crucial for maximising the levels of the bioactive compound S‐methyl cysteine sulfoxide.CONCLUSIONThe proposed MW treatment can be used by the food industry to obtain an excellent homogeneous heating of a green smoothie product retaining high levels of bioactive compounds during subsequent retail/domestic storage up to 1 month at 5 °C. © 2017 Society of Chemical Industry
How does the exosite of rhomboid protease affect substrate processing and inhibition?
Authors: Shokhen Michael , Albeck Amnon 
Published: September 2017

AbstractRhomboid proteases constitute a family of intramembrane serine proteases ubiquitous in all forms of life. They differ in many aspects from their soluble counterparts. We applied molecular dynamics (MD) computational approach to address several challenging issues regarding their catalytic mechanism: How does the exosite of GlpG rhomboid protease control the kinetics efficiency of substrate hydrolysis? What is the mechanism of inhibition by the non‐competitive peptidyl aldehyde inhibitors bound to the GlpG rhomboid active site (AS)? What is the underlying mechanism that explains the hypothesis that GlpG rhomboid protease is not adopted for the hydrolysis of short peptides that do not contain a transmembrane domain (TMD)? Two fundamental features of rhomboid catalysis, the enzyme recognition and discrimination of substrates by TMD interactions in the exosite, and the concerted mechanism of non‐covalent pre‐catalytic complex to covalent tetrahedral complex (TC) conversion, provide answers to these mechanistic questions.
Overly determined agents prevent consensus in a generalized Deffuant model on ℤ with dispersed opinions
Authors: Hirscher Timo 
Published: September 2017

AbstractDuring the last decades, quite a number of interacting particle systems have been introduced and studied in the crossover area of mathematics and statistical physics. Some of these can be seen as simplistic models for opinion formation processes in groups of interacting people. In the model introduced by Deffuantet al.(2000), agents that are neighbors on a given network graph, randomly meet in pairs and approach a compromise if their current opinions do not differ by more than a given threshold value θ. We consider the two-sided infinite path ℤ as the underlying graph and extend existing models to a setting in which opinions are given by probability distributions. Similar to what has been shown for finite-dimensional opinions, we observe a dichotomy in the long-term behavior of the model, but only if the initial narrow mindedness of the agents is restricted.
Dirichlet approximation of equilibrium distributions in Cannings models with mutation
Authors: Gan Han L. , Röllin Adrian , Ross Nathan 
Published: September 2017

AbstractConsider a haploid population of fixed finite size with a finite number of allele types and having Cannings exchangeable genealogy with neutral mutation. The stationary distribution of the Markov chain of allele counts in each generation is an important quantity in population genetics but has no tractable description in general. We provide upper bounds on the distributional distance between the Dirichlet distribution and this finite-population stationary distribution for the Wright–Fisher genealogy with general mutation structure and the Cannings exchangeable genealogy with parent independent mutation structure. In the first case, the bound is small if the population is large and the mutations do not depend too much on parent type; 'too much' is naturally quantified by our bound. In the second case, the bound is small if the population is large and the chance of three-mergers in the Cannings genealogy is small relative to the chance of two-mergers; this is the same condition to ensure convergence of the genealogy to Kingman's coalescent. These results follow from a new development of Stein's method for the Dirichlet distribution based on Barbour's generator approach and a probabilistic description of the semigroup of the Wright–Fisher diffusion due to Griffiths and Li (1983) and Tavaré (1984).
SQUARE WITH BUILT-IN DIAMOND-PLUS
Authors: RINOT ASSAF , SCHINDLER RALF 
Published: September 2017

AbstractWe formulate combinatorial principles that combine the square principle with various strong forms of the diamond principle, and prove that the strongest amongst them holds in L for every infinite cardinal.As an application, we prove that the following two hold in L:1.For every infinite regular cardinal λ, there exists a special λ+-Aronszajn tree whose projection is almost Souslin;2.For every infinite cardinal λ, there exists a respecting λ+-Kurepa tree; Roughly speaking, this means that this λ+-Kurepa tree looks very much like the λ+-Souslin trees that Jensen constructed in L.
TAMENESS AND FRAMES REVISITED
Authors: BONEY WILL , VASEY SEBASTIEN 
Published: September 2017

AbstractWe study the problem of extending an abstract independence notion for types of singletons (what Shelah calls a good frame) to longer types. Working in the framework of tame abstract elementary classes, we show that good frames can always be extended to types of independent sequences. As an application, we show that tameness and a good frame imply Shelah’s notion of dimension is well-behaved, complementing previous work of Jarden and Sitton. We also improve a result of the first author on extending a frame to larger models.
DECIDABILITY AND CLASSIFICATION OF THE THEORY OF INTEGERS WITH PRIMES
Authors: KAPLAN ITAY , SHELAH SAHARON 
Published: September 2017

AbstractWe show that under Dickson’s conjecture about the distribution of primes in the natural numbers, the theory Th (ℤ , +, 1, 0, Pr) where Pr is a predicate for the prime numbers and their negations is decidable, unstable, and supersimple. This is in contrast with Th (ℤ , +, 0, Pr, <) which is known to be undecidable by the works of Jockusch, Bateman, and Woods.
What reward does a child prefer for behaving well at the dentist?
Authors: Coxon James , Hosey Marie Therese , Newton Jonathon Timothy 
Published: September 2017

Abstract
Background:
Paediatric dentists often report using positive reinforcement to encourage their young patients to show co-operative behaviour. For effective reinforcement to take place the reward should be salient to the individual. To date, there is little research into what reward a young patient will choose when attending the dentist.

Aim:
To identify what reward children between the age of 4–8 years will choose when attending the dentist, and to determine the extent of agreement between children and caregivers in reward choice.

Method:
Observational study. Fifty-two children from different age groups (4–5 years, 6–7 years and 8 years) attending a primary-care dental clinic were asked to choose between a range of different rewards. The caregiver attending with them was also asked to anticipate the child’s preferred choice.

Results:
There was no clear favourite reward for children from both genders and different age group. However, no child chose the ‘sticker’ reward that is traditionally given out at the dentist. Overall carers agreed with the child’s choice of toy on 18 occasions (34.6%), but there were significant differences across the age groups with carers of older children showing less agreement

Conclusion:
To ensure that rewards are salient, children should be given a choice of rewards when attending the dental clinic. Parents ability to predict their child’s preferred rewards decreases as the child ages.

Clinical relevance:
A child’s motivation to co-operate during dental treatment can be increased by offering a range of rewards. Asking children to choose their reward from a limited range will increase the saliency of the reward for the child.
First observation of Hα redshifted emission in RR Lyr
Authors: Gillet D. , Mauclaire B. , Garrel T. , Lemoult T. , Mathias Ph. , de France T. , Devaux J.-S. , Boussier H. , Verilhac D. , Brabant G. , Desbordes J. , Garde O. ,   the GRRR Collaboration
Published: September 2017

Context. The so-called Hα third emission occurs around pulsation phase ϕ = 0.30. It has been observed for the first time in 2011 in some RR Lyrae stars. The emission intensity is very weak, and its profile is a tiny persistent hump in the red side-line profile.
Aims. We report the first observation of the Hα third emission in RR Lyr itself (HD 182989), the brightest RR Lyrae star in the sky.
Methods. New spectra were collected in 2013−2014 with the AURELIE spectrograph (resolving power R = 22 700, T152, Observatoire de Haute-Provence, France) and in 2016−2017 with the eShel spectrograph (R = 11 000, T035, Observatoire de Chelles, France). In addition, observations obtained in 1997 with the ELODIE spectrograph (R = 42 000, T193, Observatoire de Haute-Provence, France) were reanalyzed.
Results. The Hα third emission is clearly detected in the pulsation phase interval ϕ = 0.188−0.407, that is, during about 20% of the period. Its maximum flux with respect to the continuum is about 13%. The presence of this third emission and its strength both seem to depend only marginally on the Blazhko phase. The physical origin of the emission is probably due to the infalling motion of the highest atmospheric layers, which compresses and heats the gas that is located immediately above the rising shock wave. The infalling velocity of the hot compressed region is supersonic, almost 50 km s-1, while the shock velocity may be much lower in these pulsation phases.
Conclusions. When the Hα third emission appears, the shock is certainly no longer radiative because its intensity is not sufficient to produce a blueshifted emission component within the Hα profile. At phase ϕ = 0.40, the shock wave is certainly close to its complete dissipation in the atmosphere.