bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of MAPK‐mediated endoplasmic reticulum stress signaling in the heart during aging in senescence‐accelerated prone mice
Authors: Sreedhar Remya , Giridharan Vijayasree V. , Arumugam Somasundaram , Karuppagounder Vengadeshprabhu , Palaniyandi Suresh S. , Krishnamurthy Prasanna , Quevedo Joao , Watanabe Kenichi , Konishi Tetsuya , Thandavarayan Rajarajan A. 
Published: August 2016

AbstractHeart failure is typically related to aging as there is a definite relationship between age‐related changes in the heart and the pathogenesis of heart failure. We have previously reported the involvement of p38 mitogen‐activated protein kinase protein in cardiac function using animal models of heart failure. To further understand its relationship with aging‐induced heart failure, we have compared its expression in the hearts of senescence accelerated‐prone (SAMP8) mice and their control (SAMR1) with normal aging behavior. We have identified its activation along with reduced expression of 14‐3‐3η protein in SAMP8 mice hearts than in SAMR1 mice. To reveal the downstream signaling, we have measured the endoplasmic reticulum stress marker proteins along with some inflammatory and apoptosis markers and identified a significant increase in SAMP8 mice hearts than that of SAMR1. In addition, we have performed comet assay and revealed a significant DNA damage in the cardiomyocytes of SAMP8 mice when compared with SAMR1 mice. All these results demonstrate the role of 14‐3‐3η protein and the downstream mitogen‐activated protein kinase‐mediated endoplasmic reticulum stress, and apoptosis and DNA damage in aging‐induced cardiac malfunction in SAMP8 mice. Thus targeting this signaling might be effective in treating age‐related cardiac dysfunction. © 2016 BioFactors, 42(4):368–375, 2016
Circulating leptin and NF‐κB activation in peripheral blood mononuclear cells across the menstrual cycle
Authors: Faustmann Gernot , Tiran Beate , Maimari Theopisti , Kieslinger Petra , Obermayer‐Pietsch Barbara , Gruber Hans‐Jürgen , Roob Johannes M. , Winklhofer‐Roob Brigitte M. 
Published: August 2016

AbstractUsing the menstrual cycle as a model, this study focused on longitudinal changes and associations within a physiological network known to play a role in female fertility, including, as biologically active nodes, NF‐κB, leptin and adiponectin, β‐carotene, adipose tissue, and progesterone. In 28 women, leptin, adiponectin, β‐carotene, and progesterone concentrations, NF‐κB p65 and p50 activation in peripheral blood mononuclear cells (known to possess estrogen, progesterone and leptin receptors), total body fat (TBF) and subcutaneous adipose tissue (SAT) mass were determined at early (T1) and late follicular (T2) and mid (T3) and late (T4) luteal phase. Leptin and adiponectin concentrations were higher, while NF‐κB p65 activation was lower at T3 compared with T1. NF‐κB p65 activation was inversely related to leptin concentrations at T1, T3, and T4. β‐Carotene was inversely related to leptin (T1,T2,T4) and SAT (T1,T3,T4). NF‐κB p50 activation was inversely related to TBF (T4) and SAT (T3,T4), and leptin was positively related to TBF and SAT (T1‐T4). Progesterone was inversely related to leptin (T2,T3), adiponectin (T3), TBF (T3,T4), and SAT (T2,T3,T4). By providing evidence of luteal phase‐specific reduced NF‐κB p65 activation in women under physiological conditions, this study bridges the gap between existing evidence of a Th1‐Th2 immune response shift induced by reduced NF‐κB p65 activation and a Th1‐Th2 shift previously observed at luteal phase. For the first time, inverse regressions suggest inhibitory effects of leptin on NF‐κB p65 activation at luteal phase, along with inhibitory effects of leptin as well as adiponectin on progesterone production in corpus luteum. © 2016 The Authors BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology. 24(4):376–387, 2016
Dietary beta‐carotene and lutein metabolism is modulated by the APOE genotype
Authors: Huebbe Patricia , Lange Jennifer , Lietz Georg , Rimbach Gerald 
Published: August 2016

AbstractThe human apolipoprotein E (APOE) genotype has been suggested to interact with nutrient metabolism particularly with lipid soluble vitamins. Plasma carotenoid levels are determined by numerous dietary and genetic factors with high inter‐individual variation; however, the APOE genotype has not been systematically examined so far. Our aim was to investigate the effect of the APOE genotype on dietary carotenoid metabolism with special regard to transcriptional regulation of carotenoid absorption, cleavage and adipocyte fat storage. We supplemented targeted replacement mice expressing human APOE3 and APOE4 isoforms with dietary beta‐carotene (BC) and lutein (LUT) for 8 weeks. Plasma BC and adipose tissue BC and LUT levels were in trend lower in APOE4 than APOE3 mice, while hepatic expression of the beta‐carotene oxygenases BCO1 and BCO2 was significantly higher. In contrast to the liver, mRNA levels of proteins involved in carotenoid absorption and cleavage in the small intestinal mucosa as well as of adipogenic markers in the adipose tissue were not different between APOE3 and APOE4 mice. Our data suggest that the hepatic carotenoid cleavage activity is higher in APOE4 mice partially reducing the circulation and extra‐hepatic accumulation of intact carotenoids as compared to APOE3. Therefore we suggest considering the APOE genotype as modulator of carotenoid status in the future. © 2016 BioFactors, 42(4):388–396, 2016
Depot‐ and gender‐specific expression of NLRP3 inflammasome and toll‐like receptors in adipose tissue of cancer patients
Authors: Shimabukuro Michio , Sato Hiromi , Izaki Hirofumi , Fukuda Daiju , Uematsu Etsuko , Hirata Yoichiro , Yagi Shusuke , Soeki Takeshi , Sakaue Hiroshi , Kanayama Hiro‐omi , Masuzaki Hiroaki , Sata Masataka 
Published: August 2016

AbstractGender difference in obesity‐associated cardiovascular complication could be derived from divergent chronic inflammation. We evaluated depot‐ and gender‐specific regulation of the innate immune system in human adipose tissues. Pair samples were obtained from subcutaneous (SAT) and visceral adipose tissue (VAT) during elective surgery (Male: 35; Female: 27). Expressions of pro‐ and anti‐inflammatory adipocytokines were evaluated by semi‐quantitative qPCR. Adipose cell‐size distribution was obtained from tissue samples fixed in osmium tetroxide and analyzed by Beckman Coulter Multisizer. Levels of adiponectin were higher in SAT and VAT of female than those of male (P < 0.001 and P = 0.011, respectively). NLRP3, IL1β‐IL18, TLR2 were comparable in SAT and VAT between genders. However, TLR4 and TLR9 were increased in female SAT and VAT and HMGB1 in female VAT. Levels of adiponectin were not correlated with mean diameter of adipocyte (φ, μm) in SAT and VAT of male, but negatively well correlated in those of female (r = −0.392 and r = −0.616). Such negative correlations were also observed between levels of TLR2, TLR4, and HMGB1 and φ in female. Levels of NLRP3 and IL1β were positively correlated with φ in male, but not in female. In conclusion, Innate signals were differentially expressed in male and female adipose tissues, suggesting that the depot‐ and gender‐specific signals could be related to gender difference in chronic inflammation. © 2016 BioFactors, 42(4):397–406, 2016
Curcuma longa polyphenols improve insulin‐mediated lipid accumulation and attenuate proinflammatory response of 3T3‐L1 adipose cells during oxidative stress through regulation of key adipokines and antioxidant enzymes
Authors: Septembre‐Malaterre Axelle , Le Sage Fanny , Hatia Sarah , Catan Aurélie , Janci Laurent , Gonthier Marie‐Paule 
Published: August 2016

AbstractPlant polyphenols may exert beneficial action against obesity‐related oxidative stress and inflammation which promote insulin resistance. This study evaluated the effect of polyphenols extracted from French Curcuma longa on 3T3‐L1 adipose cells exposed to H2O2‐mediated oxidative stress. We found that Curcuma longa extract exhibited high amounts of curcuminoids identified as curcumin, demethoxycurcumin, and bisdemethoxycurcumin, which exerted free radical‐scavenging activities. Curcuma longa polyphenols improved insulin‐mediated lipid accumulation and upregulated peroxisome proliferator‐activated receptor‐gamma gene expression and adiponectin secretion which decreased in H2O2‐treated cells. Curcuminoids attenuated H2O2‐enhanced production of pro‐inflammatory molecules such as interleukin‐6, tumor necrosis factor‐alpha, monocyte chemoattractant protein‐1, and nuclear factor κappa B. Moreover, they reduced intracellular levels of reactive oxygen species elevated by H2O2 and modulated the expression of genes encoding superoxide dismutase and catalase antioxidant enzymes. Collectively, these findings highlight that Curcuma longa polyphenols protect adipose cells against oxidative stress and may improve obesity‐related metabolic disorders. © 2016 BioFactors, 42(4):418–430, 2016
Enhanced production of 2,3‐butanediol in pyruvate decarboxylase‐deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose
Authors: Choi Eun‐Ji , Kim Jin‐Woo , Kim Soo‐Jung , Seo Seung‐Oh , Lane Stephan , Park Yong‐Cheol , Jin Yong‐Su , Seo Jin‐Ho 
Published: August 2016

AbstractGalactose and glucose are two of the most abundant monomeric sugars in hydrolysates of marine biomasses. While Saccharomyces cerevisiae can ferment galactose, its uptake is tightly controlled in the presence of glucose by catabolite repression. It is desirable to construct engineered strains capable of simultaneous utilization of glucose and galactose for producing biofuels and chemicals from marine biomass. The MTH1 gene coding for transcription factor in glucose signaling was mutated in a pyruvate decarboxylase (Pdc)‐deficient S. cerevisiae expressing heterologous 2,3‐butanediol (2,3‐BD) biosynthetic genes. The engineered S. cerevisiae strain consumed glucose and galactose simultaneously and produced 2,3‐BD as a major product. Total sugar consumption rates increased with a low ratio of glucose/galactose, though, occurrence of the glucose depletion in a fed‐batch fermentation decreased 2,3‐BD production substantially. Through optimizing the profiles of sugar concentrations in a fed‐batch cultivation with the engineered strain, 99.1 ± 1.7 g/L 2,3‐BD was produced in 143 hours with a yield of 0.353 ± 0.022 g 2,3‐BD/g sugars. This result suggests that simultaneous and efficient utilization of glucose and galactose by the engineered yeast might be applicable to the economical production of not only 2,3‐BD, but also other biofuels and chemicals from marine biomass.
Solid‐in‐oil nanodispersions for transdermal drug delivery systems
Authors: Kitaoka Momoko , Wakabayashi Rie , Kamiya Noriho , Goto Masahiro 
Published: August 2016

AbstractTransdermal administration of drugs has advantages over conventional oral administration or administration using injection equipment. The route of administration reduces the opportunity for drug evacuation before systemic circulation, and enables long‐lasting drug administration at a modest body concentration. In addition, the skin is an attractive route for vaccination, because there are many immune cells in the skin. Recently, solid‐in‐oil nanodisperison (S/O) technique has demonstrated to deliver cosmetic and pharmaceutical bioactives efficiently through the skin. S/O nanodispersions are nanosized drug carriers designed to overcome the skin barrier. This review discusses the rationale for preparation of efficient and stable S/O nanodispersions, as well as application examples in cosmetic and pharmaceutical materials including vaccines. Drug administration using a patch is user‐friendly, and may improve patient compliance. The technique is a potent transcutaneous immunization method without needles.
Delays in the presentation to stroke services of patients with transient ischaemic attack and minor stroke
Authors: Hurst K , Lee R , Sideso E , Giles M , Handa A 
Published: August 2016

Abstract

Background
The UK National Institute for Health and Care Excellence (NICE) guidelines state that carotid endarterectomy should be scheduled within 2 weeks of symptoms. The recent National Stroke Strategy has reduced the time interval to 48 h. This study aimed to review the possible delays.

Methods
This study analysed patients with confirmed transient ischaemic attack (TIA) or minor stroke, referred to a single tertiary centre clinic and followed up 1 month after the event. A questionnaire was used to collect data on the rapid-access clinic pathway, and details of previous medication and treatment.

Results
Some 150 patients presented with a confirmed TIA or minor stroke during a 5-month interval (June to October 2014). Fifty-one (34·0 per cent) had a history of TIA or stroke and 35 (23·3 per cent) had undergone an ‘index’ event in the 5 days before presentation. Forty-five patients (30·0 per cent) experienced a reduction or loss of vision. Of this group, 32 had a deficit in vision only, none of whom attributed these symptoms to a cerebrovascular event. Overall 92 (61·3 per cent) of the 150 patients had a delay in presentation to medical services. Forty-seven (31·3 per cent) had residual symptoms at the clinic appointment. Eighty-eight patients (58·7 per cent) did not think they were having a stroke and 54 (36·0 per cent) were unaware of the National Stroke Strategy (FAST campaign – Face, Arm, Speech, Time).

Conclusion
Two-thirds of patients were not aware they were having a stroke, one-third were unaware of the FAST campaign and nearly one-third presented with eye symptoms. Inclusion of eye symptoms and reaffirmation of the need to react might avoid unnecessary delays in the presentation of patients with TIA and minor stroke.
Magnetite‐Binding Flagellar Filaments Displaying the MamI Loop Motif
Authors: Bereczk‐Tompa Éva , Pósfai Mihály , Tóth Balázs , Vonderviszt Ferenc 
Published: August 2016

AbstractThis work aimed at developing a novel method for fabricating 1 D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite‐binding motifs: two contained fragments of magnetosome‐associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop‐containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof‐of‐concept that flagellar filaments can be engineered to facilitate formation of 1 D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.
Bivalirudin versus unfractionated heparin during peripheral vascular interventions
Authors: Ortiz Daniel , Singh Maharaj , Jahangir Arshad , Allaqaband Suhail , Khitha Jayant , Bajwa Tanvir K. , Mewissen Mark W. 
Published: August 2016

ObjectivesThis study aimed to compare the association of access site complications and the use of unfractionated heparin versus bivalirudin during subinguinal peripheral vascular intervention.BackgroundCompared to unfractionated heparin, bivalirudin has been associated with fewer bleeding complications in patients undergoing percutaneous coronary intervention but more ischemic events. The safety and efficacy of direct thrombin inhibitors in peripheral vascular interventions is not well defined.MethodsWe compared the incidence of in‐hospital access site complications and discharge status among patients in the multicenter, prospective Vascular Quality Initiative registry who underwent peripheral vascular intervention between August 2007 and January 2014 using bivalirudin or unfractionated heparin. Propensity score matching was used to obtain a balanced cohort of 1,524 patients in each treatment group.ResultsPatients treated with bivalirudin had a significantly lower incidence of access site hematomas (2.4% vs. 3.9%, P = 0.018), shorter post‐procedural hospitalization (1.0 vs. 1.2 days, P < 0.001) and lower rates of discharge to a nursing home or rehabilitation center rather than home (7.61% vs. 9.73%, P = 0.034) when compared with unfractionated heparin‐treated patients. The incidence of in‐hospital access site occlusion, distal embolization, and mortality did not differ significantly between groups.ConclusionsPatients who received bivalirudin had lower rates of access site hematoma, shorter length of stay, and improved discharge status compared with unfractionated heparin during hospitalization for peripheral vascular intervention. Randomized comparisons of these agents are needed to confirm these findings. © 2016 Wiley Periodicals, Inc.
Hemorrhagic and ischemic outcomes of Heparin vs. Bivalirudin in carotid artery stenting: A meta‐analysis of studies
Authors: Omran Jad , Abdullah Obai , Abu‐Fadel Mazen , Gray William A , Firwana Belal , Drachman Douglas E , Mahmud Ehtisham , Aronow Herebert D , White Christopher J , Al‐Dadah Ashraf S 
Published: August 2016

IntroductionBivalirudin, has been shown to have comparable efficacy and better safety profile when compared to unfractionated heparin (UFH) in percutaneous coronary interventions. Bivalirudin's safety in carotid artery stenting (CAS) was associated with better outcomes than heparin in some studies. In this Meta analysis we examine the hemorrhagic and ischemic outcomes associated with Bivalirudin compared to UFH during CAS.MethodsA comprehensive literature search was conducted with the electronic databases MEDLINE, EMBASE, and CENTRAL. Random‐effects meta‐analysis method was used to pool risk ratio (RR) for both Heparin and Bivalirudin with 95% confidence interval (CI). Study outcomes included hemorrhagic complications; major/minor bleeding and intracranial hemorrhage (ICH) as well as ischemic complications including ischemic stroke, myocardial infarction, and 30 day mortality.ResultsA total of four studies were included enrolling 7,784 patients. Compared to UFH, Bivalirudin was associated with significantly lower major bleeding events with a relative risk (RR) of 0.53 (95% CI: 0.35–0.80; I2 = 0%). Minor bleeding events were significantly lower in the Bivalirudin group with a RR of 0.41 (95% CI: 0.2–0.82; I2 = 0%). Looking into other outcomes, there were no significant differences between anticoagulation strategies in terms of ischemic stroke (RR 0.8, with 95% CI: 0.60–1.06), intracranial hemorrhage (RR 0.73 with 95% CI: 0.27–1.98), myocardial infarction (RR 1.01 with 95% CI: 0.59–1.73) or 30 day mortality (RR 0.83 with 95% CI: 0.47–1.47).ConclusionCompared to UFH, Bivalirudin is associated with lower bleeding risk when used during CAS. © 2016 Wiley Periodicals, Inc.
Long sheath use in femoral artery catheterizations in infants <15 kg is associated with a higher thrombosis rate
Authors: Ding Linda , Pockett Charissa , Moore John , El‐Said Howaida 
Published: August 2016

ObjectivesTo examine the factors contributing to arterial thrombosis after catheterization, particularly the use of long vs. short introducer sheaths, and propose a new protocol to address risks.BackgroundPost‐procedure arterial compromise is an important concern in pediatric cardiac catheterizations. For certain interventional procedures, the use of a long sheath is necessary, however, the incidence of complications when using such sheaths has not yet been studied.MethodsThis retrospective review includes patients <15 kg who underwent femoral artery catheterization at our institution from Feb 2006 to June 2014. The study examined 29 long sheath and 40 matched interventional short sheath cases. Data collected included age, weight, time to arterial access, total sheath duration, and arterial complications. The long sheath and short sheath groups, and confounding factors, were compared for complication rate.ResultsThe incidence of arterial compromise was 38% (11/29) in the long sheath group compared to 15% (6/40) for short sheaths (P = 0.029). Arterial thrombosis was documented by ultrasound, CT, or angiography in 71% (12/17) patients. Time to arterial access and total sheath duration were not statistically significant factors. Weight was an independent risk factor, with infants <5 kg having a higher complication rate compared to infants >5 kg at 38.2% (13/34) and 11.4% (4/35), respectively (P = 0.004). Sheath length remained statistically significant after taking weight and age into account (P = 0.011).ConclusionThe use of a long sheath in infants <15 kg is a significant risk factor for arterial compromise compared to short sheaths. Lower patient weight is also a contributing factor to increased incidence of complication, particularly in those <5 kg. © 2016 Wiley Periodicals, Inc.
Functional characterization of a GFAP variant of uncertain significance in an Alexander disease case within the setting of an individualized medicine clinic
Authors: Boczek Nicole J. , Sigafoos Ashley N. , Zimmermann Michael T. , Maus Rachel L. , Cousin Margot A. , Blackburn Patrick R. , Urrutia Raul , Clark Karl J. , Patterson Marc C. , Wick Myra J. , Klee Eric W. 
Published: August 2016

Key Clinical MessageA de novo GFAP variant, p.R376W, was identified in a child presenting with hypotonia, developmental delay, and abnormal brain MRI. Following the 2015 ACMG variant classification guidelines and the functional studies showing protein aggregate formation in vitro, p.R376W should be classified as a pathogenic variant, causative for Alexander disease.
Mixed Picolinate and Quinaldinate Iron(III) Complexes for the Catalytic Oxidation of Alcohols with Hydrogen Peroxide
Authors: Tanaka Shinji , Kon Yoshihiro , Ogawa Atsuko , Uesaka Yumiko , Tamura Masanori , Sato Kazuhiko 
Published: August 2016

AbstractA series of Fe mixed picolinate and quinaldinate complexes that catalyze the H2O2 oxidation of alcohols are presented. The Fe catalysts are generated in situ by simple mixing of iron acetate with 6‐methylpicolinic acid (6‐MepicH) and 4‐chloropicolinic acid (4‐ClpicH); they showed high catalytic activity for H2O2 oxidation of 1‐phenylethanol to acetophenone. Fe complexes generated in situ as precatalysts were successfully isolated and structurally characterized. Based on the single‐crystal X‐ray analysis and electrochemical measurements of the Fe complexes, the lability of picolinate and/or quinaldinate ligand and the redox potential of FeIII were found to be important factors for the catalytic reaction.
Framework‐Copper‐Catalyzed C−N Cross‐Coupling of Arylboronic Acids with Imidazole: Convenient and Ligand‐Free Synthesis of N‐Arylimidazoles
Authors: Devarajan Nainamalai , Suresh Palaniswamy 
Published: August 2016

AbstractA convenient and environmentally benign synthesis of N‐arylimidazoles has been demonstrated by a straightforward reaction catalyzed by the unsaturated coordination sites of Cu in the copper terephthalate metal–organic framework (Cu(tpa)‐MOF). A series of N‐arylimidazoles has been synthesized in excellent yields by the C−N cross‐coupling reaction of arylboronic acids and imidazoles catalyzed by the Cu(tpa)‐MOF using ethanol as a benign solvent. The present ligand‐free catalytic system proceeds smoothly under mild conditions, avoids stoichiometric Cu reagents, tolerates many functional groups, has a wide substrate scope, and is feasible with other nitrogen heterocycles. The stability and heterogeneity of the catalyst is evidenced by the results of a heterogeneity test, and the catalyst can be reused several times without a loss of activity. The easy preparation of the catalyst, its stability, recovery by simple filtration, and reusability reveal Cu(tpa) MOF as a versatile catalyst for academic and industrial applications.