bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a Blood Spot Creatine Kinase Skeletal Muscle Isoform Immunoassay for High-Throughput Newborn Screening of Duchenne Muscular Dystrophy
Authors: Moat Stuart J , Korpimäki Teemu , Furu Petra , Hakala Harri , Polari Hanna , Meriö Liisa , Mäkinen Pauliina , Weeks Ian 
Published: February 2017

AbstractBACKGROUNDDuchenne muscular dystrophy (DMD) is a progressive, lethal X-linked neuromuscular disorder with an average worldwide incidence of 1:5000. Blood spot creatine kinase (CK) enzyme assays previously used in newborn screening programs for DMD are nonspecific because measured CK enzyme activity is attributable to 3 isoenzyme forms of CK (CK-MM, CK-MB, and CK-BB) and it is the CK-MM isoform that is found predominantly in skeletal muscle. CK-MM is increased in boys with DMD owing to muscle damage. We describe a sensitive and specific automated immunoassay for CK-MM to screen for DMD in blood spots.METHODSThe prototype assay was developed on the PerkinElmer GSP® analyzer to enable high-throughput screening. CK-MM was assayed using a solid phase, 2-site immunofluorometric system. Purified human CK-MM was used to create calibrators and controls.RESULTSThe limit of blank (LOB), detection (LOD), and quantification (LOQ) values were &lt;1, 3, and 8 ng/mL, respectively. The analytical measurement range was 4–8840 ng/mL. Interassay (n = 40) imprecision was &lt;7% across the analytical range. Cross-reactivity was &lt;5% for CK-MB and 0% for CK-BB. The mean recovery of CK-MM was 101% (range 87%–111%). Blood spots from newborn infants (n = 277) had a mean CK-MM concentration of 155 ng/mL and a 99th centile of 563 ng/mL. The mean blood spot CK-MM concentration from 10 cases of DMD was 5458 ng/mL (range 1217–9917 ng/mL).CONCLUSIONSCK-MM can be reliably quantified in blood spots. The development of this CK-MM assay on a commercial immunoassay analyzer would enable standardized and high-throughput newborn blood spot screening of DMD.
CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11
Authors: Murniasih Tutik , Bayu Asep 
Published: February 2017

Marine sponge Aaptos-aaptos is thought to produce antimicrobial aaptamine and its derivatives. To investigate whether its associated bacteria are in fact the producer of such bioactive compounds, a study of antibacterial compounds derived from Aaptos-associated bacteria was conducted. In this research, approximately 10 bacterial colonies were isolated from the sponge Aaptos aaptos. Among the bacteria isolated, the one that showed the most potential for producing antibacterial compounds was Rhodobacteracea bacterium. Extra and intracellular bacterial extract from this strain strongly inhibited the growth of pathogenic bacteria Staphylococcus aureus and Vibrio eltor, while were moderately effective against Bacillus subtilis. Optimization of antibacterial activity was conducted by culturing Rhodobacteraceae bacterium in various carbon sources such as glucose, lactose, amylum, molasses and glycerol. The highest production of biomass was obtained by culturing this bacteria in SYP (Seawater Yeast Peptone) medium, enriched with 1% glycerol as the carbon source and with a harvesting time of around 56-104 hours. The highest activity (8 U/ml) was reached when culturing this strain in SYP medium without any adding of carbon sources. Data analysis using a statistical tool indicated that carbon sources added to medium do not have a significant effect on antibacterial activity. Characterizing the compound responsible for the antibacterial properties will be the topic of further work.Keywords : carbon source, Rhodobacteracea bacterium, Aaptos aaptos, associated bacteria
“My History, Finally Invented”: Nightwood and Its Publics
Authors: Wallace Laura K. 
Published: February 2017

Abstract
Djuna Barnes’s 1937 novel Nightwood has long been a mainstay of GLBTQ, lesbian, and/or queer canons, syllabi, and reading lists. This article argues that canons, syllabi, and reading lists promise readers the experience of “feeling public.” Drawing upon magazine and newspaper reviews, scholarly journal articles, memoirs, and reviews from the literary social networking site Goodreads, this article constructs a reception history of Nightwood that demonstrates that the book has been incorporated by modernist literary publics and lesbian publics into those publics’ own histories and into their members’ everyday worldmaking practices. This article compares historical and contemporary reading practices to argue that queer texts and reading practices make GLBTQ readers feel public, even in private. Queer novels performed important worldmaking work in the early 20th century, and continue to do so. Framed by affect studies, queer studies, and reception studies, the case study of Nightwood ’s history demonstrates that categories like “queer literature” shape affective reading practices, and vice versa.
Social Pedagogy and School Community Preventing Bullying in Schools and Dealing with Diversity: Two Sides of the Same Coin
Authors: Mylonakou-Keke Iro 
Published: February 2017

This paper presents a social pedagogical research programme that has been carried out during the years of economic crisis in Greece. It focuses on preventing bullying in schools, primarily by dealing with personal beliefs about diversity as well as by expanding and strengthening emotional, communication and social skills. What may differentiate this social pedagogical programme from others on bullying in schools is that it holds that positively dealing with diversity/otherness is important in preventing bullying, and that the programme is systemic in nature; that is, it utilises multiple possibilities arising from the transdisciplinary synergy of social pedagogy and systems science and is inspired by an emerging common philosophical and epistemological perception, integration of principles, methods and practices to be derived from the combined operation, at a higher level, of the two sciences.
This programme brings together: those involved directly or indirectly with the school and the wider community; all those who seek to create a powerful and consistent communications network to establish, strengthen, and eventually be inspired by what we call the ‘social pedagogical ethos’, which will shape and establish a new ‘social pedagogical culture’.
Social Pedagogy under Very Difficult Conditions: The Case of the Multicultural School of Athens
Authors: Kalagiakos Panagiotis 
Published: February 2017

This paper presents social pedagogy under very difficult conditions in the Multicultural School of Athens. More specifically, the paper introduces the Participatory Transformative Pedagogy model developed to train our students to overcome conflicts and to learn to coexist, communicate and gradually to participate to common actions and finally to collaborate effectively. Our efforts aim to introduce cooperation among students that have come from war-torn countries, have suffered a lot and finally meet, in the same school, students ‘responsible for their suffering’; this is the environment of the first multicultural School of Athens.
Participatory Transformative Pedagogy (PaTraPe), is a promising approach for making students develop relationships as the only way to deal with the demanding requirements of their classes that value cooperation. When sustainable relationships have been established, social pedagogy methods emerge. The aim of our model is to let a ‘social pedagogical wind’ blow in the Multicultural School of Athens, so that effective communication, community collaboration and social cohesion could be established.
Invariants of the Brill–Noether curve
Authors: Castorena Abel , López Martín Alberto , Teixidor i Bigas Montserrat 
Published: February 2017

Abstract
For a projective nonsingular curve of genus g, the Brill–Noether locus

W
d
r

(
C
)

$W^r_d(C)$

parametrizes line bundles of degree d over C with at least r + 1 (linearly independent) sections. When the curve is generic and the Brill–Noether number ρ(g, r, d) equals 1, one can then talk of the Brill–Noether curve. We introduce combinatorial methods that, with help from the theory of limit linear series, allow us to find invariants of Brill–Noether loci. In particular, we explore the first two invariants of the Brill–Noether curve, giving a new way of calculating the genus of this curve and computing its gonality when C has genus 5.
Mean width of random perturbations of random polytopes
Authors: Alonso-Gutiérrez David , Prochno Joscha 
Published: February 2017

Abstract
We prove some “high probability” results on the expected value of the mean width for random perturbations of random polytopes. The random perturbations are considered for Gaussian random vectors and uniform distributions on

ℓ
p
N

$\ell_p^N$

-balls and the unit sphere.
The Lightning Electromagnetic Pulse Coupling Effect Inside the Shielding Enclosure With Penetrating Wire
Authors: Jiao Xue , Yang Bo 
Published: February 2017

AbstractTo study the lightning electromagnetic pulse (LEMP) coupling and protection problems of shielding enclosure with penetrating wire, we adopt the model with proper size which is close to the practical engineering and the two-step finite-difference time-domain (FDTD) method is used for calculation in this paper. It is shown that the coupling voltage on the circuit lead inside the enclosure increases about 34 dB, when add 1.0 m long penetrating wire at the aperture, comparing with the case without penetrating wire. Meanwhile, the waveform, has the same wave outline as the lightning current source, shows that the penetrating wire brings a large number of low frequency component into the enclosure. The coupling effect in the enclosure will reduce greatly when penetrating wire has electrical connection with the enclosure at the aperture and the coupling voltage increase only about 12 dB than the case without penetrating wire. Moreover, the results show that though the waveguide pipe can reduce the coupling effect brought by the penetrating wire, the exposing part of penetrating wire can increase the coupling when the penetrating wire outside the enclosure is longer than the waveguide pipe and the longer the exposing part is, the stronger the coupling is.
Crystal structure of tetrakis(μ2-acetato-κ2
O:O′)-bis(2,2′-sulfonyldipyrazine-κ1
N)dicopper(II), C24H24Cu2N8O12S2
Authors: Qiang Hong , Xiao Guo-Wei , Sun Xin-Zhan 
Published: February 2017

Abstract
C24H24Cu2N8O12S2, monoclinic, P21/c (no. 14), a = 10.4668(12) Å, b = 10.1315(12) Å, c = 14.9315(17) Å, β = 94.608(5)°, V = 1578.3(3) Å3, Z = 2, R
gt(F) = 0.0268, wR
ref(F
2) = 0.0796, T = 296 K.
Analysis of Rare Earth Elements in Rock and Mineral Samples by ICP-MS and LA-ICP-MS
Authors: Sindern Sven 
Published: February 2017

AbstractThe group of the rare earth elements (REEs) serves as valuable indicator of numerous geological processes such as magma formation or fluid–rock interaction. The decay systems of the radioactive REE isotopesThe inductively coupled plasma (ICP) ion source and various types of mass spectrometers (MS) represent the basis to fulfil the analytical requirements of geoscientific studies. Today, ICP-quadrupole MS and ICP-sector field MS (SFMS) with a single detector or multiple ion collection (MC-ICP-MS) are standard instruments for REE analyses in the geosciences. Due to the need for in situ analysis, laser ablation (LA)-ICP-MS has become an important trace element microprobe technique, which is widely applied for determination of REE concentrations and isotope compositions in geoscientific laboratories.The quality of concentration analysis or isotope ratio determination of REEs by ICP-MS and LA-ICP-MS is affected by many parameters. Most significant are interferences caused by polyatomic oxide and hydroxide ion species formed in the plasma as well as fractionation effects leading to non-stoichiometric behaviour during element determination or to biased isotope ratio measurements. Laser-induced fractionation and isobaric interferences have to be considered as additional effects for LA-ICP-MS. As analyte elements and matrix are unseparated, mineral standards matching the matrix of samples are a prerequisite for accurate and precise REE concentration and isotope ratio determination. Application of fs lasers instead of the more common ns lasers in LA-ICP-MS systems turns out to be a significant step to reduce laser-induced fractionation and to overcome effects of sample matrices.
Evaluation of vascular endothelial growth factor levels in rheumatoid arthritis patients, with and without joint swelling; a comparison with erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor and anti-cyclic citruillnated protein
Authors: Gümüş Alper , Coşkun Cihan , Emre Hümeyra Öztürk , Temel Musa , İnal Berrin Berçik , Seval Hatice , Döventaş Yasemin Erdoğan , Koldaş Macit 
Published: February 2017

Abstract

Introduction
The aim of our study was to investigate the vascular endothelial growth factor levels in joint swelling-positive and joint swelling-negative rheumatoid arthritis patients and to then examine the relationship between conventional parameters such as the erythrocyte sedimentation rate and the levels of C-reactive protein, rheumatoid factor, and anti-cyclic citrullinated protein.

Methods
Fifty-nine (52 women and seven men) rheumatoid arthritis patients and 25 (20 women and five men) healthy individuals volunteered for this study. The patient group was divided into two sub-groups based on whether or not they exhibited joint swelling.

Results
The levels of vascular endothelial growth factor in the joint swelling-negative group were significantly different from those in the joint swelling-positive group, but they were not different from those in the control group (p=0.001 and p=0.72, respectively). We investigated the correlation between vascular endothelial growth factor and C-reactive protein levels (r=0.37, p=0.001). We also evaluated the diagnostic adequacy of vascular endothelial growth factor and created a ROC curve. The area under the curve was calculated to be 0.767.

Conclusion
Vascular endothelial growth factor is an adequate diagnostic biomarker and can successfully be used to predict the occurrence of rheumatoid synovitis based on local inflammation.
Selbstoffenbarung und Identität. Zur grammatikalischen Struktur der „absoluten“ Ich-Bin-Worte Jesu im Johannesevangelium
Authors: Förster Hans 
Published: February 2017

Abstract:There are instances of the nominal sentence ἐγώ εἰμι in John without a predicate nominal phrase. These have been described as “absolute” ἐγώ εἰμι-phrases (cf. John 8,24). It seems that the construction breaks the rules of Greek grammar. It is commonly assumed, therefore, that these “absolute” ἐγώ εἰμι-phrases are connected with the revelation of God to Moses in Ex 3,14. However, this assumption does not take into consideration the occurrences of the ἐγώ εἰμι-phrases in other contexts. It can be argued that these short nominal sentences are in fact in accordance with the rules of Greek grammar, and that the words ἐγώ εἰμι in the Septuagint are in most cases a translation of the Hebrew personal pronoun in the first person singular. As such they can be translated in most cases as “It is I”. This analysis is important for Johannine theology; in particular, it shows that the use of these words by Jesus cannot be taken as a special revelation.
Genetic Adaptation of Schizothoracine Fish to the Phased Uplifting of the Qinghai–Tibetan Plateau
Authors: Zhang Dongsheng , Yu Mengchao , Hu Peng , Peng Sihua , Liu Yimeng , Li Weiwen , Wang Congcong , He Shunping , Zhai Wanying , Xu Qianghua , Chen Liangbiao 
Published: February 2017

AbstractMany species of Schizothoracine, a subfamily of Cyprinidae, are highly endemic to the Qinghai–Tibetan Plateau (QTP). To characterize the adaptive changes associated with the Schizothoracine expansion at high altitudes, we sequenced tissue transcriptomes of two highland and two subhighland Schizothoracines and analyzed gene evolution patterns by comparing with lowland cyprinids. Phylogenetic tree reconstruction and divergence time estimation indicated that the common ancestor of Schizothoracine fish lived ∼32.7 million years ago (MYA), coinciding with the timing of the first phase of QTP uplifting. Both high- and subhigh-Schizothoracines demonstrated elevated dN/dS ratios in the protein-coding genes compared to lowland cyprinids, from which some biological processes implicated in altitude adaptation were commonly identified. On the other hand, the highland and subhighland lineages presented drastically divergent landscapes of positively selected genes (PSGs), enriched with very different gene ontology (GO) profiles, including those in “sensory organ morphogenesis,” “regulation of protein ubiquitination,” “blood circulation,” and “blood vessel development.” These results indicated different selection pressures imposed on the highland and subhighland lineages of the Schizothoracine subfamily, with a higher number of genes in the high-altitude species involved in adaptations such as sensory perception, blood circulation, and protein metabolism. Our study indicated divergent genetic adaptations in the aquatic species facing the phased uplifting of QTP.
Parental Health Literacy and Outcomes of Childhood Nephrotic Syndrome
Authors: Borges Karlota , Sibbald Cathryn , Hussain-Shamsy Neesha , Vasilevska-Ristovska Jovanka , Banh Tonny , Patel Viral , Brooke Josefina , Piekut Monica , Reddon Michele , Aitken-Menezes Kimberly , McNaughton Ashley , Pearl Rachel J. , Langlois Valerie , Radhakrishnan Seetha , Licht Christoph P.B. , Piscione Tino D. , Levin Leo , Noone Damien , Hebert Diane , Parekh Rulan S. 
Published: February 2017

OBJECTIVE:
Determine the association of parental health literacy with treatment response among children with nephrotic syndrome.

METHODS:
This was a cohort study of children aged 1–18 with nephrotic syndrome and their parent. Health literacy was measured using the validated Short Test of Functional Health Literacy in Adults assessing reading comprehension and numeracy. Outcomes included initial relapse-free period, frequently relapsing disease, relapse rate, second-line medication use, and complete remission after therapy.

RESULTS:
Of 190 parents, 80% had adequate health literacy (score &gt;67 of 100), and higher scores were not correlated with higher education. Almost all achieved perfect numeracy scores (&gt;86%); numeracy was not associated with outcomes. After adjusting for immigration, education, and income, higher reading comprehension scores (tertile 3) compared with lower scores (tertile 1) were significantly associated with lower risk of first relapse (hazard ratio 0.67, 95% confidence interval [CI] 0.48–0.94, P trend = .02), lower odds of frequently relapsing disease (odds ratio [OR] 0.38, 95% CI 0.21–0.70, P trend = .002), lower relapse rate (rate ratio 0.77, 95% CI 0.73–0.80, P trend &lt; .001), and higher odds of complete remission after both initial steroids and cyclophosphamide (OR 2.07, 95% CI 1.36–3.16, P trend = .003; OR 5.97, 95% CI 2.42–14.7, P trend &lt; .001).

CONCLUSIONS:
Lower parental health literacy, specifically reading comprehension, is associated with higher relapse rates among children with nephrotic syndrome and fewer achieving complete remission. This underscores the importance of assessing and targeting health literacy for chronic management of childhood-onset diseases.
EDUCATION AS A PRODUCTION FUNCTION OF HUMAN RESOURCES
Authors: Ahmad Dedy 
Published: February 2017

Abstrak. Manusia sebagai salah satu faktor produksi perlu mempunyai pengetahuan dan keterampilan untuk mampu melaksanakan rangkaian kegiatan yang berhubungan dengan sasaran dan tujuan yang ingin di capai oleh suatu organisasi/institusi. Pengetahuan dan keterampilan tersebut hanya akan diperoleh melalui suatu proses pendidikan.Pendekatan tingkat pengembalian dari hasil pendidikan (rate of return approach), mengungkap bahwa penghasilan orang berpendidikan berasal dari investasi pendidikan, dan karena nya menilai penghasilan dianggap sebagai produk pendidikan. Pendidikan merupakan sebuah proses produksi bagi sumber daya manusia yang mengharapkan untuk mempunyai bekal pengetahuan dan keterampilan untuk menunjang pertumbuhan perekonomian bagi bangsanya di masa yang akan datang. Metode Penelitian ini menggunakan metode deskriptif dengan pendekatan kualitatif, dimana pembahasan kajian ini menggunakan bentuk desk study. Pendidikan dapat dipandang sebagai suatu fungsi produksi bagi pembangunan sumber daya manusia, karena dengan mengikuti proses pendidikan manusia akan mempunyai pengetahuan, keterampilan, dan keahlian sesuai dengan apa yang diharapkannya. Hal tersebut merupakan suatu investasi bagi diri manusia itu sendiri untuk dapat tumbuh dan berkembang secara totalitas dalam rangka pembangunan dalam berbagai aspek kepentingan hidup baik untuk pribadinya maupun secara nasional dan internasional.Kata Kunci : Fungsi Produksi, Pertumbuhan Ekonomi, dan Human Investment Abstract. As one of production factors, man has to manage to have knowledge and skills. That way, the power to accomplish a series of activities relating to aims and goals of an organization can be achieved. The knowledge and skills aformentioned are gained through a merely process of education. The rate of return approach as a result of the process reveals that an income of an educated man derives from educational institution, and therefore measuring one's income is considered as a product of education. A set of education proves to be a process of production fir human resources expecting to an entity of knowledge and skills to support economic growth for the nation in years to come.The method of this research is decription method.Keywords : Production Function, Economic Growth, Human Investment