bluuebunny/crossref_metadata_2025_split huggingface dataset:
The genome of the biting midgeCulicoides sonorensisand gene expression analyses of vector competence for Bluetongue virus
Authors: Morales-Hojas Ramiro , Hinsley Malcolm , Armean Irina M. , Silk Rhiannon , Harrup Lara E. , Gonzalez-Uriarte Asier , Veronesi Eva , Campbell Lahcen , Nayduch Dana , Saski Christopher , Tabachnick Walter J. , Kersey Paul , Carpenter Simon , Fife Mark 
Published: January 2018

AbstractBackgroundThe use of the new genomic technologies has led to major advances in control of several arboviruses of medical importance such as Dengue. However, the development of tools and resources available for vectors of non-zoonotic arboviruses remains neglected. Biting midges of the genusCulicoidestransmit some of the most important arboviruses of wildlife and livestock worldwide, with a global impact on economic productivity, health and welfare. The absence of a suitable reference genome has hindered genomic analyses to date in this important genus of vectors. In the present study, the genome ofCulicoides sonorensis, a vector of bluetongue virus (BTV) in the USA, has been sequenced to provide the first reference genome for these vectors. In this study, we also report the use of the reference genome to perform initial transcriptomic analyses of vector competence for BTV.ResultsOur analyses reveal that the genome is 197.4 Mb, assembled in 7,974 scaffolds. Its annotation using the transcriptomic data generated in this study and in a previous study has identified 15,629 genes. Gene expression analyses ofC. sonorensisfemales infected with BTV performed in this study revealed 165 genes that were differentially expressed between vector competent and refractory females. Two candidate genes,glutathione S-transferase(gst) and the antiviral helicaseski2, previously recognized as involved in vector competence for BTV inC. sonorensis(gst) and repressing dsRNA virus propagation (ski2), were confirmed in this study.ConclusionsThe reference genome ofC. sonorensishas enabled preliminary analyses of the gene expression profiles of vector competent and refractory individuals. The genome and transcriptomes generated in this study provide suitable tools for future research on arbovirus transmission. These provide a significant resource for these vector lineage, which diverged from other major Dipteran vector families over 200 million years ago. The genome will be a valuable source of comparative data for other important Dipteran vector families including mosquitoes (Culicidae) and sandflies (Psychodidae), and yield potential targets for transgenic modification in vector control and functional studies.
In vivoEffects of Temperature on the Heart and Pyloric Rhythms in the Crab,Cancer borealis
Authors: Kushinsky Dahlia , Morozova Ekaterina O. , Marder Eve 
Published: January 2018

Summary StatementTemperature elevation increases the frequency of the heart and pyloric rhythms of the crab,Cancer borealis, but the heart rhythm has a higher critical temperature than the pyloric rhythm.AbstractThe heart and pyloric rhythms of crustaceans have been studied separately and extensively over many years. Local and hormonal neuromodulation and sensory inputs onto these central pattern generating circuits play a significant role in the animals’ responses to perturbations, but are usually lost or removed duringin vitrostudies. To examine simultaneously thein vivomotor output of the heart and pyloric rhythms, we used photoplethysmography (PPG). In the population measured (n = 49), the heart rhythm frequencies ranged from 0.3–2.3 Hz. The pyloric rhythms varied from 0.2–1.6 Hz. During multiple hour-long recordings, many animals held at control temperature showed strong inhibitory bouts in which the heart decreased in frequency or become quiescent and the pyloric rhythm also decreased in frequency. Many animals show significant coherence in frequency between the rhythms at the frequency of the heart rhythm. We measured the simultaneous responses of the rhythms to temperature ramps by heating or cooling the saline bath while recording both the heart and pyloric muscle movements. Q10s, critical temperatures (temperatures at which function is compromised), and changes in frequency were calculated for each of the rhythms tested. The heart rhythm was more robust to high temperature than the pyloric rhythm.
Male-specific CREB signaling in the hippocampus controls spatial memory deficits in a mouse model of autism and intellectual disability
Authors: Zamarbide Marta , Mossa Adele , Wilkinson Molly K. , Pond Heather L. , Oaks Adam W. , Manzini M. Chiara 
Published: January 2018

ABSTRACTBackgroundThe prevalence of neurodevelopmental disorders is biased towards males with male: female ratios of 2:1 in intellectual disability (ID) and 4:1 in autism spectrum disorder (ASD). However, the molecular mechanisms of such bias remain unknown. While characterizing a mouse model for loss of the signaling scaffold coiled-coil and C2 domain containing 1A (CC2D1A), which is mutated in ID and ASD, we identified biochemical and behavioral differences between males and females, and explored whether CC2D1A controls male-specific intracellular signaling.MethodsCC2D1A is known to regulate phosphodiesterase 4D (PDE4D). We tested for activation PDE4D and downstream signaling molecules such as CREB in the hippocampus of Cc2d1a-deficient mice. We then performed behavioral studies in females to analyze learning and memory, social interactions, anxiety and hyperactivity. Finally, we targeted PDE4D activation with a PDE4D inhibitor to define how changes in PDE4D and CREB activity affect behavior in males and females.ResultsWe found that in Cc2d1a-deficient males PDE4D is hyperactive leading to a reduction in CREB signaling, but this molecular deficit is not present in females. Cc2d1a-deficient females only show impairment in novel object recognition, and no other cognitive and social deficits that have been found in males. Restoring PDE4D activity using an inhibitor rescues male-specific cognitive deficits, but has no effect on females.ConclusionsOur findings show that CC2D1A regulates intracellular signaling in a male-specific manner in the hippocampus leading to male-specific behavioral deficits. We propose that male-specific signaling mechanisms are involved in establishing sex bias in neurodevelopmental disorders.
Stochastic methods for inferring states of cell migration
Authors: Allen R.J. , Welch C. , Pankow N. , Hahn K. , Elston Timothy C. 
Published: January 2018

AbstractCell migration refers to the ability of cells to translocate across a substrate or through a matrix. To achieve net movement requires spatiotemporal regulation of the actin cytoskeleton. Computational approaches are neceary to identify and quantify the regulatory mechanisms that generate directed cell movement. To address this need, we developed computational tools, based on stochastic modeling, to analyze time series data for the position of randomly migrating cells. Our approach allows parameters that characterize cell movement to be efficiently estimated from time series data. We applied our methods to analyze the random migration of Mouse Embryonic Fibroblasts (MEFS). Our analysis revealed that these cells exist in two distinct states of migration characterized by differences in cell speed and persistence. Further analysis revealed that the Rho-family GTPase RhoG plays a role in establishing these two states. An important feature of our computational approach is that it provides a method for predicting the current migration state of an individual cell from time series data. Using this feature, we demonstrate that HeLa cells also exhibit two states of migration, and that these states correlate with differences in the spatial distribution of active Rac1.
Prenatal aromatase inhibition alters postnatal immune function in domestic chickens (Gallus gallus)
Authors: Simkins J.W. , Bonier F. , Benowitz-Fredericks Z.M. 
Published: January 2018

ABSTRACTIn birds, exposure to testosterone during embryonic development can suppress immune function; however, it is unclear whether this is caused by direct stimulation of androgen receptors. Estradiol is synthesized from testosterone by the enzyme aromatase, and this conversion is a necessary step in many signaling pathways that are ostensibly testosterone-dependent. Many lines of evidence in mammals indicate that estradiol can affect immune function. We tested the hypothesis that immunosuppressive effects of avianin ovotestosterone exposure are mediated by conversion to estradiol by aromatase, using Fadrozole to inhibit aromatization of endogenous testosterone during a crucial period of embryonic immune system development in domestic chickens (Gallus gallus). We then measured total IgY antibody count, response to PHA challenge, mass of thymus and bursa of Fabricius, and plasma testosterone post-hatch on days 3 and 18. We predicted that if immunomodulation by testosterone is dependent on aromatization, then Fadrozole treatment would lead to elevated immune activity by inhibiting estrogen production. Conversely, if testosterone inhibits immune function directly by binding to androgen receptors, then Fadrozole treatment would likely not alter immune function. Fadrozole treated birds had decreased day 3 plasma IgY antibody titers but there was a strong trend towards increased day 18 thymic mass. Furthermore, Fadrozole treatment generated a positive relationship between testosterone and thymic mass in males, and tended to increase day 18 IgY levels for a given bursal mass in females. There was no effect on PHA response, bursal mass, or plasma testosterone at either age. Overall, Fadrozole treated birds tended to have elevated indicators of immune function, implicating aromatization as a relevant pathway through which developmental exposure to testosterone can affect immunity.
Auditors’ professional and organizational identities and commercialization in audit firms
Authors: Broberg Pernilla , Umans Timurs , Skog Peter , Theodorsson Emily 
Published: January 2018

Purpose
The purpose of this paper is to explain how auditors’ professional and organizational identities are associated with commercialization in audit firms. Unlike previous studies exploring the consequences of commercialization in the firms, the study directs its attention toward the potential driver of commercialization, which the authors argue to be the identities of the auditors.

Design/methodology/approach
The paper is based on 374 responses to a survey distributed to 3,588 members of FAR, the professional association of accountants, auditors and advisors in Sweden. The study used established measures of organizational and professional identity and introduced market, customer and firm process orientation as aspects of commercialization. The study explored the data through descriptive statistics, principle component analysis and correlation analysis and tested the hypotheses with multiple linear regression analysis.

Findings
The findings indicated that the organizational identity of auditors has a positive association with three aspects of commercialization: market orientation, customer orientation and firm process orientation. Contrary to the arguments based on prior literature, the study has found that the professional identity of auditors is also a positively associated with commercialization. This indicates a change of the role of professional identity vis-à-vis commercialization of audit firms. The positive association between professional identity and commercial orientation could indicate the development of “organizational professionalism.” The study also found differences between the association between professional identity and commercialization in Big 4 and non-Big 4 firms. While in Big 4 firms, professional identity is positively associated only with the firm’s process orientation, in non-Big 4 firms, professional identity has a positive association with all three aspects of commercialization.

Originality/value
The paper provides insight into how auditors’ identities have influenced commercialization of audit firms and into the normalizing of commercialization within auditing. The study also developed a new instrument for measuring commercialization, one based on market, customer and firm process orientation concepts. This paper suggests that this instrument is an alternative to the observation through proxies.
“This degrading and stealthy practice”
Authors: Miley Frances Myfanwy , Read Andrew F. 
Published: January 2018

PurposeThe purpose of this paper is to make visible the relationship between accounting and stigma in the absence of accounting. This research examines how failure to implement mandatory accounting and auditing requirements in the management of indigenous wages contributed to stigmatisation of indigenous Australians and led to maladministration and unchecked financial fraud that continued for over 75 years. The accounting failures are by those charged with protecting the financial interests of the indigenous population.Design/methodology/approachAn historical and qualitative approach has been used that draws upon archival and contemporary sources.FindingsPrior research has examined the nexus between accounting mechanisms and stigma. This research suggests that the absence of accounting mechanisms can also contribute to stigma.Research limitations/implicationsThis research highlights the complex relationship between accounting and stigma, suggesting that it is simplistic to examine the nexus between accounting and stigma without considering the social forces in which stigmatisation occurs.Social implicationsThis research demonstrates decades of failed accounting have contributed to the ongoing social disadvantage of indigenous Australians. The presence of accounting mechanisms cannot eradicate the past, or fix the present, but can create an environment where financial abuse does not occur.Originality/valueThis research demonstrates that stigma can be exacerbated in the negative space created by failures or absence of accounting.
An assessment model of FM organisational performance
Authors: Abdul Mutalib Ahmad Firdauz , Sapri Maimunah , Sipan Ibrahim 
Published: January 2018

PurposeThe purpose of this paper is to improve the existing model developed by Hsu and Sabherwal (2012) by developing a new dimension of the relationships between a group of constructs (knowledge elements, mediating constructs and facility management [FM] organisational performance) in the model of FM organisational performance.Design/methodology/approachThe paper reviews the theoretical studies empirically to all of the knowledge elements (learning culture, intellectual capital and knowledge management) literature, mediating constructs (customer performance, efficiency, innovation and dynamic capabilities) and the organisational performance that relates to FM.FindingsThe paper identifies the relationships between the knowledge elements, mediating constructs and the FM organisational performance. Subsequently, a proposal of relationships was made to develop the FM organisational performance model.Research limitations/implicationsThe model provides a possible explanation of relationships between the knowledge elements, mediating constructs and the FM organisational performance. Thus, the understanding of the identified relationships will provide a new direction in improving the FM organisational performance.Originality/valueAddressing lack of research in identifying the importance of relationships between knowledge and the FM organisational performance, the paper conceptualises the potential relationships into a proposed model. The proposed model integrates with a new mediating construct into the existing research model, which is customer performance. Moreover, knowing that the nature of FM organisation is more towards the non-financial aspects, the paper investigates the nature of the efficiency and organisational performance that is not based on the financial performance, but rather the ability to optimise organisational resources, to achieve organisational goals and customer needs.
Que Sera, sera? Conceptualizing business network foresighting
Authors: Andersen Poul , Holmen Elsebeth , Pedersen Ann-Charlott 
Published: January 2018

Purpose
Networks and relationships are not stable. On the contrary, they change and are transformed by the actors who take part in them. Change and transformation result from the actions and reactions of these actors. However, a key issue is what makes the actors choose some actions and reactions while refraining from others. The paper aims to discuss these issues.

Design/methodology/approach
The authors argue that the actors’ expectations to the future of the network are formative for the actions and reactions and, furthermore, that the future expectations are formed by interaction among the actors that take part in the networks.

Findings
The authors depart from the existing foresight literature, but realign its ideas to fit with the core tenets of the IMP approach. Thereby, the purpose is twofold: to explore and conceptualize network foresight phenomena as well as to contribute to the practice of collective foresighting in business networks.

Research limitations/implications
The authors suggest research into formations of expectations in networks with a specific view to the interactive and structural effects of networks. Furthermore, the authors suggest a framework for categorizing network episodes and linking these to the formation of recognized issues and solutions.

Practical implications
The authors provide a framework for analyzing the focus of business networks in terms of solutions and issues, and analytically breaking down the interaction among these.

Originality/value
The authors introduce the concept of business network foresight, both as a distinct concept that enables us to understand change and transformation in networks, but also as a procedure for supporting actors’ strategizing efforts in business networks.
Purchasing management and the role of uncertainty
Authors: Gadde Lars-Erik , Wynstra Finn 
Published: January 2018

Purpose
The purpose of this paper is to analyze the role of uncertainty in purchasing and supply management, and the changes of this role over time.

Design/methodology/approach
The paper is based on a literature review of the development of purchasing and supply management over time and how these issues have been related to uncertainty and dependence. This examination also required analysis of the impact of other concepts from behavioral sciences: interdependence, power and control.

Findings
The paper shows that the relationship between purchasing management and uncertainty has changed substantially over time. Traditionally, uncertainty was avoided, while firms today are engaged in efforts of handling the consequences of uncertainty. This modification affected the features of buyer-supplier relationships, as well as the perspectives and the exploitation of power, control and dependence.

Originality/value
The paper demonstrates both positive and negative consequences of uncertainty, depending on the approach applied in purchasing. Moreover, the analysis shows that uncertainty cannot be avoided. Modifications of purchasing management will reduce certain types of uncertainty. But the same modification also results in increases of other forms of uncertainty.
The relationship between marketing mix and retailer-perceived brand equity
Authors: Efanny Wita , Haryanto Jony , Kashif Muhammad , Widyanto Hanif Adinugroho 
Published: January 2018

Purpose
A huge investment launching the marketing program is made by the manufacturers to establish brand loyalty with retailers and other supply chain partners; however, what is the impact of these efforts on retailer-perceived brand equity (RPBE) is scarcely investigated in a business-to-business (B2B) context. The purpose of this paper is to investigate the impact of Nu Green – a brand of tea as a manufacturer’s marketing efforts on RPBE.

Design/methodology/approach
Based on the positivist paradigm, the authors followed a survey-based approach to collect data from 125 retailers of Nu Green Tea brand from Indonesia. The collected data were rigorously analyzed by means of structural equation modeling.

Findings
The results reveal that elements of marketing efforts such as supplier image, distribution strategy, and push and pull promotions have a significant impact on the RPBE of Nu Green.

Originality/value
This research extends the understanding of retail marketing in a B2B context by investigating the impact of marketing efforts on RPBE.
Born global on blockchain
Authors: Zalan Tatiana 
Published: January 2018

PurposeThe purpose of this paper is to alert international business (IB) and international entrepreneurship (IE) researchers of a new phenomenon and novel research opportunities arising as a result of digital innovations brought about by the new, decentralized internet popularly known as “blockchain”. The paper contains a general overview of the blockchain technology and maps connections with the IB/IE literature, focusing on explaining accelerated internationalization of firms that are born global on blockchain.Design/methodology/approachThe paper is a viewpoint based on the author’s ongoing research on blockchain and fintech and reflections on the born global literature. The paper has benefited from the author’s insights through her involvement in the global blockchain community as an investor and advisor.FindingsThe author argues for establishing a theoretical link between the born global literature and the literature on the economics of information goods and platform economics to explain the pace of international growth in the context of blockchain start-ups.Research limitations/implicationsThe author urges IB/IE researchers to pay attention to research opportunities in the blockchain area, especially those related to explaining rapid internationalization of digital start-ups and a new organizational form for organizing cross-border activities known as decentralized autonomous organization.Originality/valueThree factors are shown to contribute to a rapid internationalization of blockchain start-ups: network effects, solving the chicken-and-egg problem and building an ecosystem around the evolving technology.