bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange
Authors: Baumann Martina , Gludovacz Elisabeth , Sealover Natalie , Bahr Scott , George Henry , Lin Nan , Kayser Kevin , Borth Nicole 
Published: July 2017

AbstractSite‐specific recombinase mediated cassette exchange (RMCE) enables the transfer of the gene of interest (GOI) into pre‐selected genomic locations with defined expression properties. For the generation of recombinant production cell lines, this has the advantage that screening for high transcription rates at the genome integration site would be required only once, with the possibility to reuse the selected site for new products. Here, we describe a strategy that aims at the selection of transcriptionally active genome integration sites in Chinese Hamster Ovary (CHO) cells by using alternate start codons in the surface reporter protein CD4, in combination with FACS sorting for high expressers. The alternate start codon reduces the translation initiation efficiency and allows sorting for CHO cells with the highest transcription rates, while RMCE enables the subsequent exchange of the CD4 against the GOI. We have shown that sorted cell pools with the CD4 reporter gene containing the alternate start codon CTG lead to higher GFP signals and higher antibody titers upon RMCE as compared to cell pools containing the ATG start codon of the CD4 reporter. Despite the absence of any subcloning step, the final cell pool contained the CD4 gene in a single genome integration site.
Development of aptamers against unpurified proteins
Authors: Goto Shinichi , Tsukakoshi Kaori , Ikebukuro Kazunori 
Published: July 2017

AbstractSELEX (Systematic Evolution of Ligands by EXponential enrichment) has been widely used for the generation of aptamers against target proteins. However, its requirement for pure target proteins remains a major problem in aptamer selection, as procedures for protein purification from crude bio‐samples are not only complicated but also time and labor consuming. This is because native proteins can be found in a large number of diverse forms because of posttranslational modifications and their complicated molecular conformations. Moreover, several proteins are difficult to purify owing to their chemical fragility and/or rarity in native samples. An alternative route is the use of recombinant proteins for aptamer selection, because they are homogenous and easily purified. However, aptamers generated against recombinant proteins produced in prokaryotic cells may not interact with the same proteins expressed in eukaryotic cells because of posttranslational modifications. Moreover, to date recombinant proteins have been constructed for only a fraction of proteins expressed in the human body. Therefore, the demand for advanced SELEX methods not relying on complicated purification processes from native samples or recombinant proteins is growing. This review article describes several such techniques that allow researchers to directly develop an aptamer from various unpurified samples, such as whole cells, tissues, serum, and cell lysates. The key advantages of advanced SELEX are that it does not require a purification process from a crude bio‐sample, maintains the functional states of target proteins, and facilitates the development of aptamers against unidentified and uncharacterized proteins in unpurified biological samples.
What Drives Green Product Development and How do Different Antecedents Affect Market Performance? A Survey of Italian Companies with Eco‐Labels
Authors: Dangelico Rosa Maria 
Published: July 2017

AbstractGreen products can play a key role in the achievement of sustainable development goals. Through a survey of 188 Italian companies with eco‐labeled green products, this study aims at understanding the relative importance of several motivations to develop green products, the influence of different motivations and firm characteristics on green product features (radicalness and differentiation), and which factors affect market performance of green products.This study reports a ranking of 49 motivations, highlighting that the most relevant are related to the prospect of market benefits. Results also show that product radicalness and differentiation have partially different antecedents in terms of motivations, while being a family firm positively influences only product differentiation. With regards to factors affecting market performance of green products, prospect of market benefits, availability of new technologies, firm foreign ownership, product radicalness and differentiation show a positive influence, while firm age displays a negative effect.
Does Financial Development Affect Environmental Degradation? Evidence from the OECD Countries
Authors: Halkos George E. , Polemis Michael L. 
Published: July 2017

AbstractIn this study, building a simple model that incorporates static and dynamic elements, the relationship of financial development and economic growth to environmental degradation is investigated together with the validation of the Environmental Kuznets Curve (EKC) hypothesis. Our analysis is based on an unbalanced panel data set covering the OECD countries over the period 1970–2014. Our approach thoroughly accounts for the presence of cross‐sectional dependence between the sample variables and utilizes second generation panel unit root tests in order to investigate possible cointegration relationships. The empirical findings do indicate that local (NOx per capita emissions) and global (CO2 per capita emissions) pollutants redefine the EKC hypothesis when we account for the presence of financial development indicators. Specifically, in the case of global pollution an N‐shape relationship is evident in both static and dynamic frameworks, with a very slow adjustment. Lastly, our study calls for a strengthening of the effectiveness of environmental degradation policies by ensuring sustainability of the OECD banking system in order to drastically reduce emissions. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
Thrombolysis for pulmonary embolism using the superficial veins of the upper extremity
Authors: Kusick Joseph , Salamat Judd , Sanghvi Kintur 
Published: July 2017

AbstractThis report describes a novel approach of accessing the superficial veins of the upper extremity for the treatment of pulmonary emboli (PE) with an ultrasound enhanced thrombolytic infusion catheter. In a patient suffering from saddle PE and impending right heart failure, separate basilic and cephalic venous access sites in the right arm were utilized to insert EKOS EndoWave Infusion Catheter (EKOS Corporation, Bothell, WA) insertion in each pulmonary artery (PA). This technique could be advantageous, particularly for patients at high risk for access site complications and those unable to lie supine for prolonged duration of infusion therapy.
Incidence, predictors, and clinical outcomes of early stent thrombosis in acute myocardial infarction patients treated with primary percutaneous coronary angioplasty (insights from the University of Ottawa Heart Institute STEMI registry)
Authors: Singh Kuljit , Rashid Mohammed , So Derek Y. , Glover Christopher A. , Froeschl Michael , Hibbert Benjamin , Chong Aun‐Yeong , Dick Alexander , Labinaz Marino , Le May Michel 
Published: July 2017

AbstractBackgroundEarly stent thrombosis (ST) remains an important complication of primary percutaneous intervention (PCI). To date, our information on angiographic and clinical predictors of early ST in ST‐segment elevation myocardial infarction (STEMI) patients treated with primary PCI is limited.MethodsWe tried to evaluate the incidence, predictors, and outcomes of early ST in real‐world patients treated with primary PCI. We identified all the patients presenting with STEMI between June 2004 and January 2011 who underwent primary PCI as the primary mode of revascularization. Diagnosis of ST was made as per the standard definition proposed by the Academic Research Consortium.ResultsThe incidence of early ST was 1% among 2,303 patients treated with primary PCI. Definite and probable early ST occurred in 22 and 2 patients, respectively. Patients with early ST had higher in‐hospital (P = 0.03) and 30‐day mortality (P = 0.048). The rate of cardiogenic shock (P = 0.0006) and cerebrovascular accident (P = 0.0004) was also greater in the early ST group. Smaller stent diameter and lower use of intracoronary glycoprotein IIb/IIIa inhibitor were associated with higher rate of early ST. There was a trend of higher bivalirudin use in ST group, which did not reach significance (P = 0.07) On IVUS imaging, stent malapposition and uncovered plaque area were noted in 6 out of 11 cases.ConclusionThe incidence of early ST in primary PCI cohort is low. However, it is still associated with higher mortality and morbidity. Small stent diameter and disuse of intracoronary glycoprotein IIb/IIIa inhibitor may be associated with early ST.
Control of Reversible Activation Dynamics of [Ru{η6:κ1‐C6H5(C6H4)NH2}(XY)]n+ and the Effect of Chelating‐Ligand Variation
Authors: Martínez‐Peña Francisco , Pizarro Ana M. 
Published: July 2017

AbstractThe potential use of organoruthenium complexes as anticancer drugs is well known. Herein, a family of activatable tethered ruthenium(II) arene complexes of general formula [Ru{η6:κ1‐C6H5(C6H4)NH2}(XY)]n+ (closed tether ring) bearing different chelating XY ligands (XY=aliphatic diamine, phenylenediamine, oxalato, bis(phosphino)ethane) is reported. The activation of these complexes (closed‐ to open‐tether conversion) occurs in methanol and DMSO at different rates and to different reaction extents at equilibrium. Most importantly, RuII‐complex activation (cleavage of the Ru−Ntether bond) occurs in aqueous solution at high proton concentration (upon Ntether protonation). The activation dynamics can be modulated by rational variation of the XY chelating ligand. The electron‐donating capability and steric hindrance of XY have a direct impact on the reactivity of the Ru−N bond, and XY=N,N′‐dimethyl‐, N,N′‐diethyl‐, and N,N,N′,N′‐tetramethylethylenediamine afford complexes that are more prone to activation. Such activation in acidic media is fully reversible, and proton concentration also governs the deactivation rate, that is, tether‐ring closure slows down with decreasing pH. Interaction of a closed‐tether complex and its open‐tether counterpart with 5′‐guanosine monophosphate revealed selectivity of the active (open) complex towards interaction with nucleobases. This work presents ruthenium tether complexes as exceptional pH‐dependent switches with potential applications in cancer research.
Non‐Planar [n]Cyclo‐1,8‐carbazolylenes (n=3,4,6) and [3]Cyclo‐1,8‐carbazolylenyl B, P, PO, SiPh Complexes
Authors: Yamamoto Koji , Pandit Palash , Higashibayashi Shuhei 
Published: July 2017

AbstractA new family of non‐planar heterocyclic molecules consisting of carbazoles was created. A bowl‐shaped cyclodimer, a flake‐shaped cyclotrimer, a double‐decker‐ring‐shaped cyclotetramer and a cyclohexamer were synthesized by Ni0‐mediated one‐shot cyclo‐oligomerization of 1,8‐dibromocarbazole, and were named [n]cyclo‐1,8‐carbazolylenes (n=3,4,6). [3]Cyclo‐1,8‐carbazolylene was found to act as a trivalent tridentate ligand for heteroatoms (B, P, Si), giving flake‐shaped complexes. The boron derivative acted as a Lewis acid, and the tetra‐coordinated complexes showed an unprecedented red‐shift of absorption and emission spectra by the coordination. The synthetic method, the distorted C2 or C1 symmetric shape, the trivalent tridentate coordination ability and the kinds of introduced heteroatoms of [3]cyclo‐1,8‐carbazolylenes, the electronic structure, the photophysical property, and the Lewis acidity were distinct from those of related subporphyrins/subphthalocyanines.
Adolescent body mass index and risk of colon and rectal cancer in a cohort of 1.79 million Israeli men and women: A population‐based study
Authors: Levi Zohar , Kark Jeremy D. , Katz Lior H. , Twig Gilad , Derazne Estela , Tzur Dorit , Leibovici Weissman Yaara , Leiba Adi , Lipshiez Irena , Keinan Boker Lital , Afek Arnon 
Published: July 2017

BACKGROUNDThis study examined the association between the body mass index (BMI) in late adolescence and the risk of colon and rectal cancer.METHODSThis study analyzed a cohort of 1,087,358 Jewish men and 707,212 Jewish women who underwent health examinations at the ages of 16 to 19 years between 1967 and 2002 and were followed by linkage to the national cancer registry up to 2012. Cox regression was used to estimate hazard ratios (HRs) for cancer according to age‐ and sex‐adjusted BMI percentiles from the US Centers for Disease Control and Prevention (overweight, 85th percentile to <95th percentile; obesity, ≥95th percentile).RESULTSOver a median follow‐up of 23 years, 2967 incidence cases of colorectal cancer, including 1977 among men (1403 in the colon and 574 in the rectum) and 990 among women (764 in the colon and 226 in the rectum), were identified. Overweight and obesity were associated with the risk for colon cancer among both men (HR for overweight, 1.53; 95% confidence interval [CI], 1.28‐1.84; HR for obesity, 1.54; 95% CI, 1.15‐2.06; statistically significant from a BMI of 23.4 kg/m2 [spline analysis]) and women (HR for overweight, 1.54; 95% CI, 1.22‐1.93; HR for obesity, 1.51; 95% CI, 0.89‐2.57; significant from a BMI of 23.6 kg/m2). Obesity, but not overweight, was associated with a risk for rectal cancer among men (HR, 1.71; 95% CI, 1.11‐2.65; significant from a BMI of 29.6 kg/m2) and women (HR, 2.03; 95% CI, 0.90‐4.58; significant from a BMI of 30.6 kg/m2).CONCLUSIONSBeing overweight or obese in adolescence was associated with an increased risk of subsequent colon cancers in men and women, whereas obesity was associated with rectal cancer. Cancer 2017;123:4022‐30. © 2017 American Cancer Society.
Characterization of the axon initial segment of mice substantia nigra dopaminergic neurons
Authors: González‐Cabrera Cristian , Meza Rodrigo , Ulloa Lorena , Merino‐Sepúlveda Paulina , Luco Valentina , Sanhueza Ana , Oñate‐Ponce Alejandro , Bolam J. Paul , Henny Pablo 
Published: July 2017

AbstractThe axon initial segment (AIS) is the site of initiation of action potentials and influences action potential waveform, firing pattern, and rate. In view of the fundamental aspects of motor function and behavior that depend on the firing of substantia nigra pars compacta (SNc) dopaminergic neurons, we identified and characterized their AIS in the mouse. Immunostaining for tyrosine hydroxylase (TH), sodium channels (Nav) and ankyrin‐G (Ank‐G) was used to visualize the AIS of dopaminergic neurons. Reconstructions of sampled AIS of dopaminergic neurons revealed variable lengths (12–60 μm) and diameters (0.2–0.8 μm), and an average of 50% reduction in diameter between their widest and thinnest parts. Ultrastructural analysis revealed submembranous localization of Ank‐G at nodes of Ranvier and AIS. Serial ultrathin section analysis and 3D reconstructions revealed that Ank‐G colocalized with TH only at the AIS. Few cases of synaptic innervation of the AIS of dopaminergic neurons were observed. mRNA in situ hybridization of brain‐specific Nav subunits revealed the expression of Nav1.2 by most SNc neurons and a small proportion expressing Nav1.6. The presence of sodium channels, along with the submembranous location of Ank‐G is consistent with the role of AIS in action potential generation. Differences in the size of the AIS likely underlie differences in firing pattern, while the tapering diameter of AIS may define a trigger zone for action potentials. Finally, the conspicuous expression of Nav1.2 by the majority of dopaminergic neurons may explain their high threshold for firing and their low discharge rate.
One‐Pot Hydrothermal Synthesis of Hexagonal WO3 Nanorods/Graphene Composites as High‐Performance Electrodes for Supercapacitors
Authors: Guan Xiao‐hui , Zhang Zi‐wei , Yang Liu , Wang Guang‐sheng 
Published: July 2017

AbstractTungsten oxide (WO3) as an electrode material for supercapacitors has always suffered from low capacitance and poor rate capability. In this work, a series of WO3 nanorods/graphene composites with different weight ratios of tungsten oxide nanorods (WO3 NRs) and reduced graphene oxide (rGO) are synthesized successfully through a facile one‐pot electrostatic adsorptive hydrothermal method. In these composites, rGO enhances the conductivity, transporting electrons and protons to the WO3 NRs. In addition, rGO can reinforce the structure of the WO3 NRs during frequently occurring redox reactions. At a graphene weight ratio of 1 wt %, the specific capacitance of the WO3 NRs/rGO composite is 343 F g−1 at a current density of 0.2 A g−1. Compared with pure WO3 as electrodes, the WO3 NRs/rGO composite shows excellent specific capacity, and superior rate performance and cycling stability owing to the combined action of the double layer and pseudocapacitor. This study provides a new convenient approach to promote the electrochemical performance of tungsten oxide.
Researching Corporate Social Responsibility in the Middle East: The Current State and Future Directions
Authors: Al‐Abdin Ahmed , Roy Taposh , Nicholson John D. 
Published: July 2017

AbstractCorporate social responsibility (CSR) has the potential to yield economic and social value in the Middle East (ME), especially given the current high environmental flux in the region. Although much scholarly attention has been paid to CSR issues, a key question remains about how to operate responsibly in the ME, particularly since institutional environments and stakeholders' needs vary across ME states. The purpose of this paper is to provide a systematic review of the current state of CSR in the ME. We identify 38 papers that are most pertinent to CSR in the ME and examine the main theoretical frameworks, methodologies, trajectories for further conceptual development, gaps where new research pathways need to be created, and also future research questions. From this systematic review, we reveal how attention to CSR in the ME is slowly gaining traction. A snapshot of the gaps identified include collaboration between business and non‐governmental organisations (NGOs), the impact of stakeholders and institutions on CSR, the impact of political and economic crisis on CSR, and the influence of individualistic characteristics shaping managers' CSR behaviour. In addition to such gaps, we present an agenda for future research. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
How Does Entrepreneurial and International Orientation Influence SMEs' Commitment to Sustainable Development? Empirical Evidence from Spain and Mexico
Authors: Ayuso Silvia , Navarrete‐Báez Francisco Ernesto 
Published: July 2017

AbstractThis paper aims to shed light on the relationship between the entrepreneurial behaviour of small and medium‐sized enterprises (SMEs) and their commitment to sustainable development (SD). In order to analyse the determinants of SD engagement at the organisational level, we draw on the resource‐based view of the firm and examine specifically the effects of entrepreneurial orientation and internationalisation, using survey data from Spanish and Mexican SMEs. Our results reveal that entrepreneurial orientation is positively associated with SD engagement, particularly regarding the environment, human resources, and community involvement. However, we could only observe a positive effect of SME internationalisation on SD engagement in Mexican but not in Spanish firms, suggesting that this effect is context‐dependent and related to institutional pressures. We discuss the implications of these findings for scholars and practitioners. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
SA 8000 as a Tool for a Sustainable Development Strategy
Authors: Santos Gilberto , Murmura Federica , Bravi Laura 
Published: July 2017

AbstractIn today's global markets, issues related to environmental sustainability, ethics, working environment conditions and safety, stakeholders' relations and the protection of workers' rights have greater importance on business performances. For this reason, the aim of this research is to analyze the concept of corporate social responsibility, investigating the perception of Italian companies about Social Accountability 8000, a voluntary International Standard used as a tool to certify companies' commitment on social aspects. The survey began September 15, 2016 and answers were accepted until November 15, 2016. Two hundred twenty‐one Italian SA8000 certified companies participated to the survey reaching a response rate of 20.4%. Using a questionnaire submitted to certified companies, the main reasons which have driven companies to certification have been investigated, highlighting that the motivations which drive companies to be certified are both of an internal and external type: an improved image is the first, followed by the willingness to have a better working environment. Among the experienced benefits resulted from the study there are ‘reduction of labor conflicts’ and ‘achievement of a competitive advantage over competitors’, while limitations seem to be scarcely perceived and are defined especially by the lack of staff and management training. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
1D and 2D NMR Spectroscopy of Bonding Interactions within Stable and Phase‐Separating Organic Electrolyte–Cellulose Solutions
Authors: Clough Matthew T. , Farès Christophe , Rinaldi Roberto 
Published: July 2017

AbstractOrganic electrolyte solutions (i.e. mixtures containing an ionic liquid and a polar, molecular co‐solvent) are highly versatile solvents for cellulose. However, the underlying solvent–solvent and solvent–solute interactions are not yet fully understood. Herein, mixtures of the ionic liquid 1‐ethyl‐3‐methylimidazolium acetate, the co‐solvent 1,3‐dimethyl‐2‐imidazolidinone, and cellulose are investigated using 1D and 2D NMR spectroscopy. The use of a triply‐13C‐labelled ionic liquid enhances the signal‐to‐noise ratio for 13C NMR spectroscopy, enabling changes in bonding interactions to be accurately pinpointed. Current observations reveal an additional degree of complexity regarding the distinct roles of cation, anion, and co‐solvent toward maintaining cellulose solubility and phase stability. Unexpectedly, the interactions between the dialkylimidazolium ring C2−H substituent and cellulose become more pronounced at high temperatures, counteracted by a net weakening of acetate–cellulose interactions. Moreover, for mixtures that exhibit critical solution behavior, phase separation is accompanied by the apparent recombination of cation–anion pairs.