bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pacientų, sergančių Vernerio sindromu, odos opų chirurginis gydymas: literatūros apžvalga
Authors: Radavičiūtė Viltė 
Published: September 2024

Introduction. Werner’s syndrome is a rare autosomal recessive disorder characterised by premature ageing phenotypes, inclu­ding skin ulcers resistant to conservative treatment. Purpose. To review the cases of surgical treatment of skin ulcers in patients with Werner’s syndrome published in the scientific literature. Methods. Scientific articles were searched using Google Scholar, PubMed and DeepDyve databases, applying the keyword “ulcer” and the word combination “Werner’s syndrome”. Based on the inclusion and exclusion criteria, 16 scientific publications were included in the review. Results. Depending on the location and nature of the ulcers in patients with Werner’s syndrome, different surgical options are available, with different surgical outcomes, such as partial or complete ulcer healing, non-healing or recurrence. Conclusions. In skin grafting, ulcers are more likely to heal incompletely or temporarily. Therefore additional treatment options can be used such as vacuum negative pressure therapy, Kirschner wires or removal of the upper layer of bone. Other grafts, such as artificial skin, are more reliable, especially in large joint areas. However, a critical assessment of each individual case is necessary before choosing surgical treatment, as these patients have different degrees of tissue blood flow, muscle atrophy and general condition.
A foodborne outbreak caused by staphylococcal enterotoxins in cheese sandwiches in northern Italy
Authors: Manila Bianchi Daniela , Romano Angelo , Tramuta Clara , Distasio Palma , Decastelli Lucia 
Published: September 2024

Staphylococcus aureus can produce numerous toxins, including staphylococcal enterotoxins, namely, SEs, SEA to SEE, SEG to SEI, and SER to SET. They have demonstrated emetic activity and can cause food poisoning outbreaks (FBOs). Here, we report a multidisciplinary investigation conducted by authorities competent in food safety in collaboration with veterinary and health and hygiene services. The issue was foodborne intoxication which involved eight members (aged 12–74 years) of the same family. Notification reported that time to symptom onset was about 3 hours after lunch, which suggests that the food contained preformed toxins. Ham and cheese used for the preparation of sandwiches consumed by all symptomatic relatives were suspected to be the source of FBO. The day after symptoms appeared, samples were collected at the restaurant and delivered to the food safety laboratory. All analyses performed on official food samples are validated according to ISO 17025:2017 and accredited. The cheese subsamples were all positive for coagulase-positive staphylococci CPS (range: 1.1*103 CFU/g to 8.1*103 CFU/g). The pooled sample tested positive for staphylococcal enterotoxin D (SED) at 0.649 ng/g. This concentration can cause symptoms of FBO. Following the notification of suspected food poisoning, a rapid response by the district food safety agency is necessary. In the case described here, the multidisciplinary collaboration facilitated the collection of samples at the food business plant aimed at identifying a suspected source of intoxication and to withdraw unsafe food from the market.
Tropospheric ozone pollution: Implication for food security and crop nutrition in South Asia
Authors: Qasim Sadaf , Ahmad Muhammad Nauman , Zia Afia , Alam Sahib , Riaz Muhammad , Aziz Tariq , Zahra Nureen , Alhomrani Majid , Alsanie Walaa F. , Alamri Abdulhakeem S. , Alshammary Amal F. 
Published: September 2024

The study was conducted on ambient ozone (O3), the most phyto-toxic air pollutant, and its effects on the growth and quality of okra (Abelmoschus esculentus) and peas (Pisum sativaum) grown in Northern Pakistan during the summer and winter of 2018. Okra was subjected to ambient O3 levels ranging from 43 to 63 ppb during the summer, with a mean O3 concentration of 55 ppb, while peas experienced lower winter concentrations of 15–25 ppb, with a mean O3 concentration of 19 ppb. The results indicated significant impacts on the growth and nutritional quality of crops, especially okra. Anti-ozonant ethylene diurea (EDU) was used for soil drenching to protect okra and green peas from O3 damage. Okra showed notable enhancements of 20%, 20%, 29%, and 13% in ash, protein, fiber, and non-fiber carbohydrates (NFE), respectively. Increase in plant height, leaf numbers, pod length, and dry weight was observed in EDU-treated okra plants. Conversely, peas exhibited less variation, although melioration was observed in plant height, pod numbers, length, and weight with EDU treatment. It was concluded that the concentration of ambient O3 in Peshawar is toxic enough to cause significant damage to crop growth and production. The stark difference in O3 impact during different seasons suggests that higher summer concentrations could severely compromise crop quality. This elicits significant concerns regarding food security in South Asia, especially for summer crops that can jeopardize future food security. It is recommended that further research be conducted on the effects of O3 on other regional crops to assess fully its implications for agricultural sustainability in the area.
Potato peel as substrate for Single Cell Protein in animal feed by submerged fermentation
Authors: Kiran Nimra , Yunus Fakhar-Un-Nisa , Nadeem Muhammad , Manzoor Farkhanda , Nelofar Rubina , Sarwar Abid , Ullah Najeeb , Aziz Tariq , S. Alamri Abdulhakeem , Alsanie Walaa F. , Alhomrani Majid 
Published: September 2024

Single-cell protein (SCP) has gained attention in scientific research as a vital source of protein in animal feed and human food. Taking into account the importance of SCP, an attempt was made in the current research to grow Rhizopus oligosporus and Saccharomyces cerevisiae biomass by selecting potato peel as a substrate. The potato peel was treated with sulfuric acid to form potato peel hydrolysate following acid hydrolysis. In 1.0-L potato peel hydrolysate, 2.5-g/L KH2PO4, 0.5-g/L MgSO4, 0.5-g/L NaCl, and 10 g/L yeast extract were taken to prepare growth media. The moisture content of potato peel was examined by following standard procedure of the Association of Official Agricultural Chemists (AOAC). The Kjeldahl method was used to determine the protein content, and glucose was examined by using the dinitrosalicylic acid (DNS) method. Optimization of SCP yield was accomplished by following the ‘one factor at a time’ method. The effect of each parameter, such as incubation period, temperature and pH on SCP production was analyzed. Bioreactor scale-up production of SCP was performed in a jar fermenter. To enhance SCP, yield co-culture of Saccharomyces cerevisiae and Rhizopus oligosporus was used in a bioreactor that yielded 19 g/L of SCP. Thus, as a nutritional supplement for living organisms, SCP can effectively fulfill protein scarcity globally.
Effects of tea and coffee on tooth discoloration
Authors: Kim Soyeon , Son Ji Eun , Larnani Sri , Sim Hye-Young , Yun Pil-Young , Kim Young-Jae , Park Young-Seok 
Published: September 2024

Tooth discoloration because of dietary habits is a serious concern, particularly from tea and coffee consumption. This study investigated the effects of these beverages on tooth discoloration, focusing on their key chemical components. Bovine enamel specimens were immersed in green and black tea samples and Arabica and Robusta coffee samples for 1–72 h. High-performance liquid chromatography analyzed catechins, theaflavins, and chlorogenic acids, while spectrophotometry measured discoloration. Multiple regression analysis (significance at p < 0.05) revealed that all beverages caused significant tooth discoloration, with black tea showing the most pronounced effect because of its theaflavin content.
Evaluation of the pharmacokinetics of an experimental combined form of IFNα and IFNγ for intranasal use
Authors: Kravchenko E. V. , Bezrukova E. V. , Vorobeychikov E. V. , Skvortsov N. V. 
Published: September 2024

Polypous rhinosinusitis is a chronic inflammatory disease of the nasal mucosa and paranasal sinuses. Recombinant interferon has antiproliferative activity and corrects the deficiency of endogenous regulatory molecules, which allows us to consider this class of immunotropic drugs as a promising component of conservative immunotherapy for polyposis rhinosinusitis. Purpose of the study: To select the optimal composition of the experimental composition of interferons and evaluate their pharmacokinetic parameters in laboratory animals.For laboratory evaluation of the effectiveness of the auxiliary components of the proposed composition in creating a local effect, Wistar rats in the amount of 30 animals were selected, to which the proposed form of the drug (IFN1) was administered once intranasally. The control group included 30 animals that were alternately administered intranasally with a similar dose of IFNα2b and IFNγ dissolved in water for injection (IFN2). Quantitative determination of interferon concentration in blood samples was carried out using the enzyme immunoassay method. To assess the dependence of changes in concentrations in the blood of animals on the time elapsed after their administration, standard pharmacokinetic models were used. Next, the integral from the initial moment of time to infinity was determined, which corresponded to the area under the pharmacokinetic curve and made it possible to calculate a number of pharmacokinetic characteristics.The following indicators were obtained: 1) Area under the pharmacokinetic curve (AUCt) ng/ml/min – IFN1 = 683.0; IFN2 = 1707.7. 2) Absorption constant (Kp) – IFN1 = 0.13096; IFN2 = 0.03836. 3) Suction constant (Kel) – IFN1 = 0.00177; IFN2 = 0.00317. 4) Clearance (Cl) ml/min – IFN1 = 129.35; IFN2 = 51.73.Based on the differences in the values of pharmacokinetic parameters (AUCt) for the IFN1 and IFN2 preparations, it can be concluded that the use of a composition based on chitosan, succinic acid and DMSO leads to a pronounced retention of interferon in the nasal mucosa, which provides a pronounced local therapeutic effect and allows for fewer systemic adverse reactions. This composition is suitable for further clinical studies of the effectiveness of immunotherapy for polyposis rhinosinusitis.
Flow cytometric analysis of the interaction of monoclonal antibodies to various epitopes of the HSP70 molecule with intracellular and membrane-associated forms of this protein
Authors: Ovsyanikova O. V. , Shustova O. A. , Grechikhina M. V. , Sapozhnikov A. M. 
Published: September 2024

Currently, a large amount of data has demonstrated that many types of tumor cells, in contrast to their non-transformed forms, are characterized by the translocation of intracellular heat shock proteins 70 kDa (HSP70) to the surface of the plasma membrane. This made it possible to classify HSP70 exposed on the cell surface as a tumor-associated antigen and was the basis for searching the opportunities for the practical use of this phenomenon in clinical oncology. A significant argument in favor of the prospects of such studies was the discovered ability of HSP70, present on the surface of target cells, to enhance the cytotoxic activity of NK cells. In this regard, the work of many research groups is devoted to developing approaches to increasing the level of membrane-associated HSP70 in tumor tissues. At the same time, given the presence of such proteins on the surface of many types of tumor cells, the use of monoclonal antibodies that interact with HSP70 molecules for antitumor therapy can be considered as one of the promising approaches. It is well known that antibody preparations that selectively interact with cancer cells can be used for targeted antitumor immunotherapy. Previously, we obtained a panel of six B cell hybridomas producing monoclonal antibodies to the inducible and constitutive forms of human HSP70, directed to various epitopes of this molecule. It is significant that three varieties of hybridomas produced antibodies with specificity for binding sites on the C-terminal domain of the HSP70 molecule, and the second trio of monoclonal antibodies were specific to epitopes on the N-terminal domain of HSP70, while almost all known commercial antibodies interact only with C-terminal fragments of HSP70. In this work, we conducted a comparative study of the binding of the obtained antibodies to these proteins localized in different types of cells, both in the intracellular space and on the cell surface.The results obtained allow us to consider the identified varieties of monoclonal antibodies that most effectively recognize surface HSP70 as a promising basis for the creation of new drugs for antitumor immunotherapy.
Immunobiological efficacy of a new sodium polyprenyl phosphate based medicine for the treatment and prevention of experimenrtal metabolic syndrome
Authors: Pronin A. V. , Cheknev S. B. , Nikolaeva T. N. , Kozlov V. V. , Kozhevnikova T. N. , Vostrova E. I. , Grigorieva E. A. , Sosnovskaya O. Yu. , Sarycheva M. A. , Vostrov A. V. , Narovlyansky A. N. , Sanin A. V. 
Published: September 2024

The aim of the research was to study the population composition of the splenic lymphoid cells, to assess the functional activity of lymphocytes as well as the state of the gastrointestinal tract microbiota in experimental modeling of metabolic syndrome (MS).The studies were conducted using two experimental models of MS and hyperlipidemia (HL), based on prolonged drinking of animals with 20% aqueous fructose solution with added cholesterol and intraperitoneal administration of Poloxamer 407 to mice, respectively.The results of the experiments indicate a change in the population composition of splenocytes (decrease in CD4+ and CD8+T cells, activation of CD4+CD25+FoxP3+Thed cells), accompanied by a decrease in T cell activity and increased proliferation of B lymphocytes, impaired production of IL-15 and IL-22, as well as lipid and carbohydrate metabolism (adiponectin, leptin, insulin), which serves as a prerequisite for the development of chronic inflammation, which is a pathogenetic sign of MS.We found changes in the intestinal microbiota of mice characteristic of the manifestation of metabolic dysbiosis – an increase in the representation of Firmicutes bacteria (staphylococci, streptococci, enterococci) in the biomaterial, changes in the content of facultative (E. coli) and transient (Enterobacter) microflora.In order to develop a new kind of medicine for therapy and prevention of HL and MS, we used a combination of sodium polyprenyl phosphate (PP) and beta-sitosterol (BSS), polyisoprenoid derivatives of plant origin.More pronounced changes were found in the splenocyte population composition and activation parameters of Treg cells in HL modeling compared with the MS model. The introduction of PP and BSS has an immunocorrective effect during treatment.The therapeutic effect of this drug, as well as the prevention of the MS symptoms, is accompanied by normalization of the microbiota state.The data obtained indicate the prospects of using PP and BSS for the prevention and treatment of HL and MS in order to influence the leading links in the pathogenesis of metabolic disease.
Нормативно закрепленные инструменты стимулирования низовой гражданской активности
Authors: Исаева Елена Александровна , Соколов Александр Владимирович 
Published: September 2024

The article describes the results of a study aimed at identifying normatively established tools for stimulating the development of positive civic engagement. The authors present the results of a comparative study of the legislation of the constituent entities of the Russian Federation, including examples of the most successful stimulation of volunteerism, charity, and territorial public self-government. The authors put forward the thesis that for the successful development of volunteerism, charity, territorial public self-government and other systemic institutions of civil society, an important factor is the state of the non-profit sector of the region itself, which the author reveals using the example of the Yaroslavl region.
Métodos de organización del trabajo, racionalidad neoliberal y Salud Mental
Authors: Wlosko Miriam 
Published: September 2024

El trabajo constituye el escenario privilegiado de la mediación entre el campo social y la economía psíquica (Dejours 1990). Por ello puede contribuir tanto al mantenimiento del equilibrio psíquico y l a economía psicosomática, como incidir en la fragilización subjetiva en la aparición de diversos trastornos psicopatológicos.
Es en épocas de crisis en las que se pone claramente de manifiesto la centralidad del trabajo en la estructuración de la subjetividad. Mediante argumentos en los que se presenta al mercado y la competitividad como fatales e inevitables; la racionalidad neoliberal legitima la implementación de políticas manageriales de reestructuración organizacional que operan mediante la precarización laboral, la amenaza de despido y modalidades de gestión mediante el uso del terror.
Procesos transformadores en salud mental y producción de cuidados. La problemática de las personas en situación de calle como analizador crítico (CABA, Argentina)A pesar de los cambios normativos introducidos en materia de salud mental y derechos humanos
Authors: Cantor Paula , Valero Ana Silvia , Torricelli Flavia 
Published: September 2024

A pesar de los cambios normativos introducidos en materia de salud mental y derechos humanos en Argentina, su expresión a través de políticas públicas es aún dispar e incompleta.
En particular, la problemática de las personas en situación en calle (PSC) en Ciudad Autónoma de Buenos Aires (CABA) constituye un emergente que desafía las perspectivas teóricas y las estrategias estatales para la viabilización de las políticas públicas en salud mental, para el acceso y protección de derechos. Desde la perspectiva de producción de cuidados, la aproximación a estas problemáticas conduce a repensar las herramientas para el abordaje en salud mental con atención a la complejidad de las trayectorias de vida, y a correr el foco de los efectores de salud como espacio exclusivo productor de cuidados. Este trabajo se propone examinar el entrecruzamiento entre la producción de cuidados y las perspectivas de abordaje en salud mental con atención particular a la problemática de las PSC. Para ello, se seleccionaron dos casos de PSC de la CABA con problemáticas asociadas al campo de la salud mental. Ambos tuvieron lugar en dos momentos muy diferentes, durante los años 2013 y 2021; uno de ellos en contexto de pandemia por COVID 19, y presentaron una configuración contrastante en cuanto al entramado social, político y económico. El trabajo se funda sobre la base de una selección intencional de artículos periodísticos y se sustenta en un análisis temático cualitativo.
La problemática de las PSC se presenta como un analizador crítico para profundizar y orientar las transformaciones en salud mental acorde con el plexo normativo.
Sentipensares a propósito del II Encuentro de la Red Latinoamericana y Caribeña de Salud Mental Colectiva y Buen Vivir
Authors: Gozález Natalia 
Published: September 2024

El 18 de julio del 2023 la Red Latinoamericana y Caribeña de Salud Mental Colectiva y Buen Vivir (red temática de Salud Mental, de la Asociación Latinoamericana de Medicina Social y Salud Colectiva [ALAMES]) celebró en Argentina su II Encuentro, en el marco del XVII Congreso de ALAMES, el cual tuvo por lema “Sindemia, reconfiguración en el mundo y lucha por el buen vivir”, en la Facultad de Ciencias Sociales de la Universidad de Buenos Aires, entre el 17 y 21 de julio del 2023. Este encuentro reunió a personas trabajadoras, profesionales, investigadoras, usuaries, referentes de organizaciones comunitarias y referentes históricos del campo de la salud mental de 11 países de Latinoamérica. Compañeres con quienes al encontrarnos vamos construyendo tramas, habitando otros modos de hacer.
Levav: un luchador necesario
Authors: Kraut Alfredo 
Published: September 2024

El doctor Itzhak Levav -psiquiatra, sanitarista, epidemiólogo e investigador- falleció el 20 de mayo en Jerusalén. Tenía 85 años. No es sencillo para mí colaborar con semblanzas de quien fuera un gran amigo y mentor en este apasionante mundo del derecho y la salud mental, materia poco abordada desde la perspectiva jurídica en nuestro país. Le debo al Dr. Levav un especial agradecimiento, respeto y gratitud a quien fuera un referente indiscutido en la lucha por mejorar o transformar la vida de las personas con padecimientos entales, un colectivo estigmatizado, jurídicamente hiposuficiente, avasallado y sojuzgado. Insistía en su prédica por la eficacia de los derechos humanos (hoy retóricos en su mayoría), especialmente el derecho a la salud, a tener voz y a vivir dignamente en la comunidad. Su dimensión ética, su compromiso, su capacidad de trabajo y su rigor científico lo caracterizaron desde muy joven.
Enhancing the Health and Lifespan of Honey Bees (Apis mellifera L.) with an Innovative Soybean-Based Diet Supplemented with Porcine Blood1
Authors: Danmek Khanchai , Hongsibsong Surat , Klaithin Kanokwan , Klaitanoad Supakhom , Auearchin Thanchanok , Wu Ming-Cheng , Jung Chuleui , Chuttong Bajaree 
Published: September 2024

Abstract
This study investigated the effect of soy-based diets supplemented with porcine blood (PB) on the diet's external morphology, nutritional efficacy, and health impacts on honey bees, Apis mellifera L. (Hymenoptera: Apidae). Scanning electron microscopy revealed that the control diet (maize bee bread) displayed ruptured granules with small particles, whereas the soy-based diets showed varying degrees of protein gelatinization and aggregation depending on the presence of PB. Nutritional analysis indicated that maize bee bread was used as a positive control contained 12.2% protein and provided 312.89 kcal/100 g, whereas the formulated soy-based diets with skim milk or PB showed 13–15% protein, meeting the required needs for optimal honey bee health and development. Survival and longevity assessments showed no significant difference in lifespan between honey bees fed with artificial diets and the control, but all treatment groups outlived the negative control, which fed only on syrup. Moreover, the diameter of the hypopharyngeal gland acini, a key indicator of the nutritional state and health in honey bees, was significantly larger in honey bees fed protein-enriched diets compared with those receiving only syrup. These findings underscore the potential of soy-based artificial protein diets, especially when enhanced with PB, to support honey bee health and longevity, comparable with natural pollen sources.
As repercussões na saúde mental de crianças e adolescentes vulneráveis no período pós-pandêmico: revisão integrativa
Authors: Lara Nunes de Souza Ester , Cristine Carvalho Moura Karen , Aparecida de Barros Junqueira Marcelle , Rodrigues da Silva Mônica , Silveira Miranda Frank José , Denari Giuliani Carla 
Published: September 2024

Objetivo: compreender como o período da pandemia influenciou a saúde mental de crianças e adolescentes e suas repercussões no período pós-pandêmico. Método: revisão integrativa realizada nas bases de dados SciELO, PubMed, Web of Science e Embase, de 2020 a abril de 2024, utilizando os descritores Covid-19, saúde mental, equidade social, crianças, adolescentes e vulnerabilidade. Resultados: de 22 artigos inicialmente levantados foram consideradas seis publicações, das quais os achados mostraram como repercussões pós-pandêmicas: ansiedade, depressão, estresse, violência, relacionamento familiar, problemas de saúde mental pré-existentes como fatores ou transtornos que agravam em curto, médio e longo prazo o cuidado psicossocial do jovem. Conclusão: a pandemia de COVID-19 provocou ou agravou sinais e sintomas de impacto negativo à saúde mental de crianças e adolescentes. Observou-se uma lacuna em produções sobre os efeitos pós pandemia, destacando a necessidade de pesquisas adicionais. É essencial desenvolver estratégias para a identificação e o tratamento de transtornos mentais, promover a readaptação social e fornecer suporte emocional adequado.