bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bisphenol A Affects on the Functional Properties and Proteome of Testicular Germ Cells and Spermatogonial Stem Cells in vitro Culture Model
Authors: Karmakar Polash Chandra , Kang Hyun-Gu , Kim Yong-Hee , Jung Sang-Eun , Rahman Md. Saidur , Lee Hee-Seok , Kim Young-Hyun , Pang Myung-Geol , Ryu Buom-Yong 
Published: September 2017

AbstractThe endocrine disruptor bisphenol A (BPA) is well known for its adverse effect on male fertility. Growing evidence suggests that BPA may interact with testicular germ cells and cause infertility as a result of its estrogenic activity. Objective of current in vitro study was to investigate the proliferation, survivability and stemness properties of mouse testicular germ cells exposed to BPA, and to evaluate possible expression of cellular proteome. Our results showed that germ cell viability and proliferation were not affected by low concentrations (0.01, 0.1, 1, and 10 µM) although significant reduction observed at 100 µM BPA. Germ cell self-renewal and differentiation related marker proteins expression found unchanged at those concentrations. When BPA-exposed germ cells were transplanted into recipient testes, we observed fewer colonies at higher concentrations (10 and 100 µM). Additionally, a significant frequency of recombination failure during meiosis was observed in 10 µM BPA-exposed germ cell transplanted recipient. Moreover, experiment on continuous BPA-exposed and 100 µM BPA-recovered germ cells suggested that spermatogonial stem cells are more potential to survive in adverse environment. Finally, scrutinizing differentially expressed cellular proteins resulted from our proteomic analysis, we conclude that BPA exposure might be associated with several health risks and infertility.
CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines
Authors: Yu Qing-hui , Wang Baike , Li Ning , Tang Yaping , Yang Shengbao , Yang Tao , Xu Juan , Guo Chunmiao , Yan Peng , Wang Qiang , Asmutola Patiguli 
Published: September 2017

AbstractQuickly and precisely gain genetically enhanced breeding elites with value-adding performance traits is desired by the crop breeders all the time. The present of gene editing technologies, especially the CRISPR/Cas9 system with the capacities of efficiency, versatility and multiplexing provides a reasonable expectation towards breeding goals. For exploiting possible application to accelerate the speed of process at breeding by CRISPR/Cas9 technology, in this study, the Agrobacterium tumefaciens-mediated CRISPR/Cas9 system transformation method was used for obtaining tomato ALC gene mutagenesis and replacement, in absence and presence of the homologous repair template. The average mutation frequency (72.73%) and low replacement efficiency (7.69%) were achieved in T0 transgenic plants respectively. None of homozygous mutation was detected in T0 transgenic plants, but one plant carry the heterozygous genes (Cas9/*-ALC/alc) was stably transmitted to T1 generations for segregation and genotyping. Finally, the desired alc homozygous mutants without T-DNA insertion (*/*-alc/alc) in T1 generations were acquired and further confirmed by genotype and phenotype characterization, with highlight of excellent storage performance, thus the recessive homozygous breeding elites with the character of long-shelf life were generated. Our results support that CRISPR/Cas9-induced gene replacement via HDR provides a valuable method for breeding elite innovation in tomato.
Double deficiency of Trex2 and DNase1L2 nucleases leads to accumulation of DNA in lingual cornifying keratinocytes without activating inflammatory responses
Authors: Manils Joan , Fischer Heinz , Climent Joan , Casas Eduard , García-Martínez Celia , Bas Jordi , Sukseree Supawadee , Vavouri Tanya , Ciruela Francisco , de Anta Josep Maria , Tschachler Erwin , Eckhart Leopold , Soler Concepció 
Published: September 2017

AbstractThe cornification of keratinocytes on the surface of skin and oral epithelia is associated with the degradation of nuclear DNA. The endonuclease DNase1L2 and the exonuclease Trex2 are expressed specifically in cornifying keratinocytes. Deletion of DNase1L2 causes retention of nuclear DNA in the tongue epithelium but not in the skin. Here we report that lack of Trex2 results in the accumulation of DNA fragments in the cytoplasm of cornifying lingual keratinocytes and co-deletion of DNase1L2 and Trex2 causes massive accumulation of DNA fragments throughout the cornified layers of the tongue epithelium. By contrast, cornification-associated DNA breakdown was not compromised in the epidermis. Aberrant retention of DNA in the tongue epithelium was associated neither with enhanced expression of DNA-driven response genes, such as Ifnb, Irf7 and Cxcl10, nor with inflammation. Of note, the expression of Tlr9, Aim2 and Tmem173, key DNA sensor genes, was markedly lower in keratinocytes and keratinocyte-built tissues than in macrophages and immune tissues, and DNA-driven response genes were not induced by introduction of DNA in keratinocytes. Altogether, our results indicate that DNase1L2 and Trex2 cooperate in the breakdown and degradation of DNA during cornification of lingual keratinocytes and aberrant DNA retention is tolerated in the oral epithelium.
Increased TREM-2 expression on the subsets of CD11c+ cells in the lungs and lymph nodes during allergic airway inflammation
Authors: Hall Sannette C. , Agrawal Devendra K. 
Published: September 2017

AbstractDendritic cells (DCs) are professional APCs that traffic to the draining lymph nodes where they present processed antigens to naïve T-cells. The recently discovered triggering receptor expressed on myeloid cells (TREM)-2 has been shown to be expressed on DCs in several disease models, however, its role in asthma is yet to be elucidated. In the present study, we examined the effect of allergen exposure on TREM-2 expression in the airways and on DC subsets in the lung and lymph nodes in murine model of allergic airway inflammation. Sensitization and challenge with ovalbumin reproduced hallmark features of asthma. TREM-2 mRNA expression in the whole lung was significantly higher in the OVA-sensitized and -challenged mice which was associated with increased protein expression in the lungs. Analysis of CD11c+MHC-IIhi DCs in the lung and draining lymph nodes revealed that allergen exposure increased TREM-2 expression on all DC subsets with significantly higher expression in the lymph nodes. This was associated with increased mRNA expression of Th2 and Th17 cytokines. Further analyses showed that these TREM-2+ cells expressed high levels of CCR-7 and CD86 suggesting a potential role of TREM-2 in mediating maturation and migration of DC subsets in allergic airway inflammation.
Bisoprolol protects myocardium cells against ischemia/reperfusion injury by attenuating unfolded protein response in rats
Authors: Zhang Chengcheng , He Songqing , Li Yanming , Li Feng , Liu Zhengbing , Liu Jing , Gong Jianbin 
Published: September 2017

AbstractBisoprolol (B) exerts potential cardioprotective effects against myocardial ischemia/reperfusion (I/R) injury. Unfolded protein response (UPR) attenuates I/R injury induced apoptosis by reducing oxidative damage and inflammation response. The current study investigated whether the protective effects of bisoprolol resulted from modulating UPR and anti-inflammatory during myocardial I/R condition and elucidated its potential mechanisms. Sprague-Dawley rats were treated with B in the absence or presence of the injected UPR activator dithiothreitol (DTT) and then subjected to myocardial I/R surgery. In vitro, cultured H9C2 cells were pretreated with B or DTT and then subjected to simulate ischemia reperfusion (SIR) operation. Bisoprolol conferred cardioprotective effects by improving postischemic cardiac function, decreasing infarct size, reducing apoptotic index, diminishing serum creatine kinase and lactate dehydrogenase levels, suppressing TNF-α and IL-6 secretion, inhibiting UPR signal pathways and downregulating caspase-12 and caspase-3 expressions. Consistently, B conferred similar antioxidative and anti-inflammatory effects against SIR injury in cultured H9C2 cardiomyocytes. Pretreatment with DTT or C/EBP homologous protein (CHOP) overexpression mediated by lentivirus administration both abolished these effects. In summary, our results demonstrate that Bisoprolol protects myocardium cells against ischemia/reperfusion injury partly by attenuating unfolded protein response.
Parametric study on kink instabilities of twisted magnetic flux ropes in the solar atmosphere
Authors: Mei Z. X. , Keppens R. , Roussev I. I. , Lin J. 
Published: September 2017

Aims. Twisted magnetic flux ropes (MFRs) in the solar atmosphere have been researched extensively because of their close connection to many solar eruptive phenomena, such as flares, filaments, and coronal mass ejections (CMEs). In this work, we performed a set of 3D isothermal magnetohydrodynamic (MHD) numerical simulations, which use analytical twisted MFR models and study dynamical processes parametrically inside and around current-carrying twisted loops. We aim to generalize earlier findings by applying finite plasma β conditions.
Methods. Inside the MFR, approximate internal equilibrium is obtained by pressure from gas and toroidal magnetic fields to maintain balance with the poloidal magnetic field. We selected parameter values to isolate best either internal or external kink instability before studying complex evolutions with mixed characteristics. We studied kink instabilities and magnetic reconnection in MFRs with low and high twists.
Results. The curvature of MFRs is responsible for a tire tube force due to its internal plasma pressure, which tends to expand the MFR. The curvature effect of toroidal field inside the MFR leads to a downward movement toward the photosphere. We obtain an approximate internal equilibrium using the opposing characteristics of these two forces. A typical external kink instability totally dominates the evolution of MFR with infinite twist turns. Because of line-tied conditions and the curvature, the central MFR region loses its external equilibrium and erupts outward. We emphasize the possible role of two different kink instabilities during the MFR evolution: internal and external kink. The external kink is due to the violation of the Kruskal-Shafranov condition, while the internal kink requires a safety factor q = 1 surface inside the MFR. We show that in mixed scenarios, where both instabilities compete, complex evolutions occur owing to reconnections around and within the MFR. The S-shaped structures in current distributions appear naturally without invoking flux emergence. Magnetic reconfigurations common to eruptive MFRs and flare loop systems are found in our simulations.
No Difference in Functional Outcomes after Total Knee Arthroplasty with or without Pinless Navigation
Authors: Chen Jerry , Chong Hwei , Pang Hee , Tay Darren , Chia Shi-Lu , Lo Ngai , Yeo Seng , Zhu Meng 
Published: September 2017

AbstractThis study aims to investigate the functional outcomes of pinless navigation (BrainLAB VectorVision Knee 2.5 navigation system; Munich, Germany) as an intraoperative alignment guide in total knee arthroplasty (TKA). A prospective, 24-month follow-up study of 100 patients who were scheduled and randomized into two groups, the pinless navigation and conventional surgery, was conducted. All TKAs were performed with the surgical aim of achieving neutral coronal alignment with the 180-degree mechanical axis. The outcomes measured in this study were Oxford Knee Score (OKS), Knee Society Score (KSS), Short Form-36 (SF-36), and range of motion (ROM). At 24-month postoperatively, four and two patients were lost to follow-up from the pinless navigation group and conventional group, respectively. There were no significant differences in absolute scores of the OKS, KSS, and ROM, as well as changes from preoperative baseline, between pinless navigation and conventional groups at both 6 and 24 months postoperatively. Pinless navigation results in comparable functional outcomes as conventional TKA at 6 and 24 months postoperatively.
Multiple endokrine Neoplasie
Authors: Schaaf Ludwig , Raue Friedhelm 
Published: September 2017

AbstractMultiple endocrine neoplasia type 1 and 2 are hereditary cancer syndromes. They are characterized by the occurrence of many benign and malignant tumor types, in MEN1 parathyroid tumors, pituitary tumors, and pancreas tumors, in MEN2 medullary thyroid carcinoma, pheochromocytoma, and parathyroid tumors. The autosomal dominant inherited tumor syndromes are caused by mutations in the MEN1 gene, a tumor suppressor gene, and mutations in the RET gene, an activated oncogene, in MEN2. The clinical expression of the different tumors can vary within and between families, with a good genotype-phenotype correlation in MEN2. Early diagnosis and therapy is possible by using biochemical and imaging screening in the families. Early thyroidectomy in young patients with MEN2 results in a high cure rate of MTC.
Prioritäten deutscher Onkologen. Auswertung der „Klug entscheiden“-Umfrage der Deutschen Gesellschaft für Hämatologie und Medizinische Onkologie (DGHO)
Authors: Krause Stefan , Oldenburg Michael , Hallek Michael , Neubauer Andreas 
Published: September 2017

Zusammenfassung
Hintergrund Die Deutsche Gesellschaft für Hämatologie und Medizinische Onkologie (DGHO) hatte im Rahmen der „Klug entscheiden“-Initiative ihre Mitglieder zur Über- und Unterversorgung befragt. Aus der großen Menge an Antworten ergab sich ein überraschend deutliches Meinungsbild, das für die hier vorliegende Darstellung quantitativ ausgewertet wurde.
Methodik Die Bewertung der vorformulierten Empfehlungen wird mittels beschreibender Statistik dargestellt. Die Freitextantworten wurden in einem iterativen Verfahren in 3 Ebenen nach der medizinischen Aussage und ergänzend nach der allgemeinen Zielrichtung kategorisiert.
Ergebnisse 492 DGHO-Mitglieder schlossen die Online-Umfrage in der kurzen Frist von 14 Tagen vollständig ab. Die vorgeschlagenen Empfehlungen zur Vermeidung von Überversorgung fanden eine breite inhaltliche Zustimmung (77 % bis 88 % der Teilnehmer). In den 1598 Freitextantworten wurde der Verzicht auf unnötigen Einsatz von Ressourcen und unnötige Belastung von Patienten in Übereinstimmung mit unseren Empfehlungen gefordert. Eine mögliche Unterversorgung wurde weniger auf dem Gebiet teurer medizinischer Maßnahmen gesehen (17 % der Antworten) als vielmehr bei weichen Faktoren: „sprechende“ Medizin, individuelle Therapieführung, Supportivtherapie und Palliativtherapie sowie soziale Ressourcen (62 %).
Schlussfolgerung Aus den Antworten ergab sich ein deutliches Votum für ein Mehr an sprechender Medizin in der onkologischen Versorgung. Jeder beteiligte Arzt kann hierzu beitragen. Strukturelle Verbesserungen in der Vergütung und Weiterbildung sind jedoch wünschenswert.
100 Jahre Hautklinik an der Georg-August-Universität in Göttingen: Die Moulagensammlung
Authors: Ude-Koeller S. , Fuchs T. 
Published: September 2017

ZusammenfassungMoulagen wurden über viele Jahrzehnte als Lehrmittel verwendet und schienen im dermatologischen Unterricht bis in die 50er-Jahre unverzichtbar. Sie wurden dennoch von der Farbfotografie abgelöst. Mit diesen historischen Aspekten wird ein Bogen geschlagen zu aktuellen Entwicklungen, d. h. zu Perspektiven der interdisziplinären Moulagen-Forschung, zu Problemen bzw. Herausforderungen ihrer öffentlichen Präsentation, zur erneuten Integration der Moulagen in die dermatologische Lehre. Grundlage hierfür ist die seit 1917 unter dem ersten dermatologischen Göttinger Lehrstuhlinhaber Prof. Dr. Rudolf Erhard Riecke (1869 – 1939) in Göttingen angelegte Moulagen-Sammlung. In dem Zusammenhang erfolgten erstmalig biografische Nachforschungen zu dem in der Klinik tätigen Mouleur August Leonhardt. Dabei bestätigte sich die Bedeutung dieser Berufsgruppe als maßgebliche Sammlungsakteure.
Schwangerschaft bei Niereninsuffizienz
Authors: Kurschat Christine , Benzing Thomas 
Published: September 2017

ZUSAMMENFASSUNGEine reduzierte Nierenfunktion beeinflusst die Fertilität und geht in der Schwangerschaft mit einem erhöhten Risiko für Mutter und Kind
einher. Dieses Risiko hängt von der Schwere der Nierenfunktionseinschränkung ab. Frauen mit geringer Niereninsuffizienz und normalem
Blutdruck haben meist kein erhöhtes Risiko. Frauen mit schwerer Niereninsuffizienz haben in bis zu 92 % der Fälle Komplikationen, daher
sollte in diesem Fall mit der Schwangerschaft gewartet werden, bis eine Nierentransplantation erfolgreich durchgeführt wurde, denn diese
führt zu einer deutlichen Reduktion der Komplikationen. Wenn eine nierentransplantierte Patientin einen Schwangerschaftswunsch äußert,
sollte die medikamentöse Therapie vor dem Eintreten der Schwangerschaft bereits umgestellt werden. Dies betrifft i. d. R. immunsuppressive
und antihypertensive Medikamente. Durch eine Schwangerschaft können sich vorbestehende Glomerulonephritiden verschlechtern, insbesondere die
Lupusnephritis und die Nierenbeteiligung bei systemischer Sklerose. Harnwegsinfekte müssen in der Schwangerschaft differenziert antibiotisch
therapiert werden.
Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity
Authors: Omori Yoshihiro , Kubo Shun , Kon Tetsuo , Furuhashi Mayu , Narita Hirotaka , Kominami Taro , Ueno Akiko , Tsutsumi Ryotaro , Chaya Taro , Yamamoto Haruka , Suetake Isao , Ueno Shinji , Koseki Haruhiko , Nakagawa Atsushi , Furukawa Takahisa 
Published: September 2017

Significance
Each retinal rod and cone photoreceptor cell subtype expresses a distinct set of genes including the genes encoding phototransduction components such as opsins and transducins. This subtype-specific gene expression is essential for achieving proper function in each photoreceptor cell. While the various cone and rod photoreceptor cell gene expressions are regulated by multiple transcription factors, whether epigenetic regulation plays an important role for photoreceptor cell-specific gene expression has been unclear. In the current study, we found that a photoreceptor-specific protein, Samd7, functions as a component of the epigenetic gene-silencing complex and is essential for establishing rod photoreceptor cell identity and function by silencing nonrod gene expression in developing rod photoreceptor cells.
A functional approach to self-points and self-reference in a deaf signing child and the (dis)continuity issue in child language
Authors: Caët Stéphanie , Limousin Fanny , Morgenstern Aliyah 
Published: September 2017

AbstractBased on her observation of two deaf children acquiring American Sign Language (ASL) who stopped pointing to persons at around 12 months and then produced reversal errors,Petitto (1987)argued that the discontinuous development of gestures and signs gives support to the hypothesis that language does not arise from general cognitive processes. However, since then, a large amount of studies on hearing children have suggested that early pointing was strongly related to later language abilities. In this paper, we follow up on these socio-cognitive approaches, with a dataset comparable to Petitto’s. We study the development of pointing and self-reference in a deaf child acquiring French Sign Language (LSF). We focus on self-reference rather than self-points, and suggest that, despite the apparent discontinuity in the production of self-points, there is continuity in the establishment of self-reference. In our data, the child produces self-points early on. She then uses predicates without overt subject before entering more complex syntax by combining predicates and self-points. The deaf signing child constructs self-reference similarly to speaking children and uses specific forms provided by her linguistic environment according to her cognitive, social and linguistic development.
Microstructure and wear properties of spark plasma sintered 316L-30W composites
Authors: Tan Chao , Wang Sen , Chen Yong , Wang Guoyu , Ji Lina , Tong Yangang , Duan Xuan-Ming 
Published: September 2017

316L-30W composites were successfully fabricated via spark plasma sintering at 1550°C to evaluate their potential as the interlayer between W and 316L stainless steel in fusion reactors. The effect of holding time on the microstructure and its subsequent effects on the fracture morphology and wear properties of the composites were investigated. The results show that more W particles melted and reacted with 316L steel as the holding time increased from 1 to 5 min. The generation of voids was mainly caused by the differences in diffusivity and the coefficient of thermal expansion between W and 316L. The 316L-30W composite held for 3 min had a smaller and more stable friction coefficient, indicating that its interface was firmly bonded and homogeneous. This paper is part of a thematic issue on Nuclear Materials.
The Overlap of Sleep Disturbance and Depression in Primary Care Patients Treated with Buprenorphine
Authors: Garnaat Sarah L. , Weisberg Risa B. , Uebelacker Lisa A. , Herman Debra S. , Bailey Genie L. , Anderson Bradley J. , Sharkey Katherine M. , Stein Michael D. 
Published: September 2017

Background Sleep disturbance is common among patients receiving long-term opioid therapies, such as methadone maintenance. However, little is known about sleep disturbances in patients receiving medication treatment with buprenorphine. We sought to determine the frequency of subjective sleep disturbance in a sample of patients receiving medication treatment and to examine clinical factors related to sleep disturbance. Methods Participants were 328 persons receiving buprenorphine at 3 primary care sites. Sleep difficulty was assessed 2 questions adapted from the Patient Health Questionnaire-9 (PHQ-9) item assessing sleep. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression Scale (CESD)-10 and PHQ-2. In addition, information was gathered on participant demographics and treatment characteristics. Demographics, buprenorphine treatment history, and depressive symptoms were compared for those with and without self-reported sleep difficulty. Logistic regression was used to estimate the adjusted association of sleep disturbance with these correlates. Results Seventy-one percent of persons receiving medication treatment with buprenorphine in the present study reported sleep difficulty. Persons reporting sleep disturbance reported shorter time in buprenorphine treatment and more depressed mood compared with those without sleep difficulty ( p < .01). Men were significantly less likely to report disturbed sleep than women (odds ratio [OR] = 0.57, 95% confidence interval [CI]: 0.33, 0.98). Sleep disturbance was not associated significantly with age, ethnicity, educational attainment, or buprenorphine dose. Conclusions Sleep disturbance is common in patients receiving medication treatment with buprenorphine and is associated with more depressive symptoms as well as a shorter duration of medication treatment. Future research, using subjective and objective sleep measures, is warranted to understand whether sleep disturbance is mitigated by longer buprenorphine treatment and whether difficulty sleeping predicts buprenorphine discontinuation among patients seeking treatment for opioid dependence.