bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-modal noise compensation in audiovisual words
Authors: Baart Martijn , Armstrong Blair C. , Martin Clara D. , Frost Ram , Carreiras Manuel 
Published: February 2017

AbstractPerceiving linguistic input is vital for human functioning, but the process is complicated by the fact that the incoming signal is often degraded. However, humans can compensate for unimodal noise by relying on simultaneous sensory input from another modality. Here, we investigated noise-compensation for spoken and printed words in two experiments. In the first behavioral experiment, we observed that accuracy was modulated by reaction time, bias and sensitivity, but noise compensation could nevertheless be explained via accuracy differences when controlling for RT, bias and sensitivity. In the second experiment, we also measured Event Related Potentials (ERPs) and observed robust electrophysiological correlates of noise compensation starting at around 350 ms after stimulus onset, indicating that noise compensation is most prominent at lexical/semantic processing levels.
Enhancement of 5-aminolevulinic acid-based fluorescence detection of side population-defined glioma stem cells by iron chelation
Authors: Wang Wenqian , Tabu Kouichi , Hagiya Yuichiro , Sugiyama Yuta , Kokubu Yasuhiro , Murota Yoshitaka , Ogura Shun-ichiro , Taga Tetsuya 
Published: February 2017

AbstractCancer stem cells (CSCs) are dominantly responsible for tumor progression and chemo/radio-resistance, resulting in tumor recurrence. 5-aminolevulinic acid (ALA) is metabolized to fluorescent protoporphyrin IX (PpIX) specifically in tumor cells, and therefore clinically used as a reagent for photodynamic diagnosis (PDD) and therapy (PDT) of cancers including gliomas. However, it remains to be clarified whether this method could be effective for CSC detection. Here, using flow cytometry-based analysis, we show that side population (SP)-defined C6 glioma CSCs (GSCs) displayed much less 5-ALA-derived PpIX fluorescence than non-GSCs. Among the C6 GSCs, cells with ultralow PpIX fluorescence exhibited dramatically higher tumorigenicity when transplanted into the immune-deficient mouse brain. We further demonstrated that the low PpIX accumulation in the C6 GSCs was enhanced by deferoxamine (DFO)-mediated iron chelation, not by reserpine-mediated inhibition of PpIX-effluxing ABCG2. Finally, we found that the expression level of the gene for heme oxygenase-1 (HO-1), a heme degradation enzyme, was high in C6 GSCs, which was further up-regulated when treated with 5-ALA. Our results provide important new insights into 5-ALA-based PDD of gliomas, particularly photodetection of SP-defined GSCs by iron chelation based on their ALA-PpIX-Heme metabolism.
Fast and Quantitative T1ρ-weighted Dynamic Glucose Enhanced MRI
Authors: Schuenke Patrick , Paech Daniel , Koehler Christina , Windschuh Johannes , Bachert Peter , Ladd Mark E. , Schlemmer Heinz-Peter , Radbruch Alexander , Zaiss Moritz 
Published: February 2017

AbstractCommon medical imaging techniques usually employ contrast agents that are chemically labeled, e.g. with radioisotopes in the case of PET, iodine in the case of CT or paramagnetic metals in the case of MRI to visualize the heterogeneity of the tumor microenvironment. Recently, it was shown that natural unlabeled D-glucose can be used as a nontoxic biodegradable contrast agent in Chemical Exchange sensitive Spin-Lock (CESL) magnetic resonance imaging (MRI) to detect the glucose uptake and potentially the metabolism of tumors. As an important step to fulfill the clinical needs for practicability, reproducibility and imaging speed we present here a robust and quantitative T1ρ-weighted technique for dynamic glucose enhanced MRI (DGE-MRI) with a temporal resolution of less than 7 seconds. Applied to a brain tumor patient, the new technique provided a distinct DGE contrast between tumor and healthy brain tissue and showed the detailed dynamics of the glucose enhancement after intravenous injection. Development of this fast and quantitative DGE-MRI technique allows for a more detailed analysis of DGE correlations in the future and potentially enables non-invasive diagnosis, staging and monitoring of tumor response to therapy.
Human CD64-targeted non-viral siRNA delivery system for blood monocyte gene modulation
Authors: Yong Seok-Beom , Kim Hyung Jin , Kim Jang Kyoung , Chung Jee Young , Kim Yong-Hee 
Published: February 2017

AbstractA subset of phagocytes including inflammatory monocytes in blood migrate and give rise to macrophages in inflammatory tissues which generated the idea that blood monocytes are the therapeutic targets for drug delivery. Fc gamma receptor I (CD64) is a membrane receptor for the Fc region of immunoglobulin G, primarily expressed on monocyte-lineage, and H22 a monoclonal antibody for human CD64 had shown rapid blood monocyte binding and occupation in clinical studies. Small interfering RNA-mediated gene silencing as a therapeutic has been proposed and is a promising strategy in terms of its “knock-down” ability on the target gene prior to translation. However, its instability and off-targeting effect must be overcome for success in clinical studies. In this study, we developed a non-viral delivery system composed of oligo-nona-arginine (9R) and anti-human CD64 single chain antibodies (H22) for human monocyte-specific siRNA delivery. A targeted and efficient siRNA delivery mediated by anti-CD64 scFv-9R was observed in CD64 positive human leukemia cells, THP-1. With primary human blood cells, anti-CD64 scFv-9R mediated gene silencing was quantitatively confirmed representing blood monocyte selective gene delivery. These results demonstrate the potential of anti-CD64 scFv-9R mediated siRNA delivery for the treatment of human inflammatory diseases via blood monocytes gene delivery.
Shrimp AHPND-causing plasmids encoding the PirAB toxins as mediated by pirAB-Tn903 are prevalent in various Vibrio species
Authors: Xiao Jinzhou , Liu Liyuan , Ke Yiyun , Li Xiefei , Liu Yunfei , Pan Yingjie , Yan Shuling , Wang Yongjie 
Published: February 2017

AbstractAcute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease caused by pirAB toxins encoded by a plasmid found in Vibrio parahaemolyticus. The pirAB toxins are the homologs of the Photorhabdus insect-related (Pir) toxins. Here, we report the complete sequences of the AHPND-causing plasmid isolated from V. owensii, as well as those of its 11 siblings (pVH family). In addition, we also included 13 related plasmids (pVH-r family) without the pirAB genes isolated from a variety of species within the Vibrio Harveyi clade. Furthermore, the pirAB-Tn903 composite transposon was identified in pVH, and both ends of the transposon appeared to have inserted simultaneously into the ancestor plasmid at different sites. The homologue counterparts of pirAB were also detected in a non-pVH plasmid in V. campbellii. Taken together, our results provide novel insights into the acquisition and evolution of pirAB as well as related plasmids in the Vibrio Harveyi clade.
Bronchoalveolar Lavage Proteomics in Patients with Suspected Lung Cancer
Authors: Carvalho Ana Sofia , Cuco Célia Marina , Lavareda Carla , Miguel Francisco , Ventura Mafalda , Almeida Sónia , Pinto Paula , de Abreu Tiago Tavares , Rodrigues Luís Vaz , Seixas Susana , Bárbara Cristina , Azkargorta Mikel , Elortza Felix , Semedo Júlio , Field John K. , Mota Leonor , Matthiesen Rune 
Published: February 2017

AbstractLung cancer configures as one of the deadliest types of cancer. The future implementation of early screening methods such as exhaled breath condensate analysis and low dose computed tomography (CT) as an alternative to current chest imaging based screening will lead to an increased burden on bronchoscopy units. New approaches for improvement of diagnosis in bronchoscopy units, regarding patient management, are likely to have clinical impact in the future. Diagnostic approaches to address mortality of lung cancer include improved early detection and stratification of the cancers according to its prognosis and further response to drug treatment. In this study, we performed a detailed mass spectrometry based proteome analysis of acellular bronchoalveolar lavage (BAL) fluid samples on an observational prospective cohort consisting of 90 suspected lung cancer cases which were followed during two years. The thirteen new lung cancer cases diagnosed during the follow up time period clustered, based on liquid chromatography-mass spectrometry (LC-MS) data, with lung cancer cases at the time of BAL collection. Hundred and thirty-tree potential biomarkers were identified showing significantly differential expression when comparing lung cancer versus non-lung cancer. The regulated biomarkers showed a large overlap with biomarkers detected in tissue samples.
Quality Evaluation Focusing on Tissue Fractal Dimension and Chemical Changes for Frozen Tilapia with Treatment by Tangerine Peel Extract
Authors: He Qi , Yang Zhao , Gong Bin , Wang Jingjing , Xiao Kaijun , Yang Shang-Tian 
Published: February 2017

AbstractThis work aimed to establish an effective approach to evaluate the quality of frozen fish, focusing on changes in fish tissue structure and chemical composition during storage. Fresh tilapia samples were treated by coating with tangerine peel (TP) extract and then stored at −4, −8 and −18 °C, respectively, for 40 days. The frozen fish tissues were analyzed for structural and chemical changes. Fractal dimension, which quantifies the porous structure formed in the tissue samples, texture properties including hardness and springiness, and moisture content and water activity all decreased during the storage, while the extents of lipid oxidation, measured as peroxide value and thiobarbituric acid concentration, and protein degradation, monitored with total volatile basic nitrogen and trichloroacetic acid soluble peptides, increased. The change rates of these parameters decreased with decreasing the storage temperature and by applying TP extract. A model was developed for predicting fractal dimension, which indicated the quality of preserved tilapia and thus can be used to predict the shelf life under different storage temperatures. The results demonstrated that TP extract could extend the shelf life of frozen tilapia by 35–45% by inhibiting changes in tissue structure, moisture loss, lipid oxidation and protein degradation during frozen storage.
Development of a Novel Simulation Reactor for Chronic Exposure to Atmospheric Particulate Matter
Authors: Ye Jianhuai , Salehi Sepehr , North Michelle L. , Portelli Anjelica M. , Chow Chung-Wai , Chan Arthur W. H. 
Published: February 2017

AbstractEpidemiological studies have shown that air pollution is associated with the morbidity and mortality from cardiopulmonary diseases. Currently, limited experimental models are available to evaluate the physiological and cellular pathways activated by chronic multi-pollutant exposures. This manuscript describes an atmospheric simulation reactor (ASR) that was developed to investigate the health effects of air pollutants by permitting controlled chronic in vivo exposure of mice to combined particulate and gaseous pollutants. BALB/c mice were exposed for 1 hr/day for 3 consecutive days to secondary organic aerosol (SOA, a common particulate air pollutant) at 10–150 μg/m3, SOA (30 μg/m3) + ozone (65 ppb) or SOA + ozone (65 ppb) + nitrogen dioxide (NO2; 100 ppb). Daily exposure to SOA alone led to increased airway hyperresponsiveness (AHR) to methacholine with increasing SOA concentrations. Multi-pollutant exposure with ozone and/or NO2 in conjunction with a sub-toxic concentration of SOA resulted in additive effects on AHR to methacholine. Inflammatory cell recruitment to the airways was not observed in any of the exposure conditions. The ASR developed in this study allows us to evaluate the chronic health effects of relevant multi-pollutant exposures at ‘real-life’ levels under controlled conditions and permits repeated-exposure studies.
Copulatory courtship by internal genitalia in bushcrickets
Authors: Wulff Nadja C. , van de Kamp Thomas , dos Santos Rolo Tomy , Baumbach Tilo , Lehmann Gerlind U. C. 
Published: February 2017

AbstractMale genital organs are among the fastest evolving morphological structures. However, large parts of the male’s genitalia are often hidden inside the female during mating. In several bushcricket species, males bear a pair of sclerotized genital appendices called titillators. By employing synchrotron-based in vivo X-ray cineradiography on mating couples, we were able to visualize titillator movement and spermatophore attachment inside the female. Titillators are inserted and retracted rhythmically. During insertion the titillator processes tap the soft and sensillae-covered dorsal side of the female’s flap-like genital fold, which covers the opening of the female’s genitalia, without tissue penetration. Titillators thus appear to be initially used for stimulation; later they may apply pressure that forces the female’s genital fold to stay open, thereby aiding mechanically in spermatophore transfer.
Spatially resolved air-water emissions tradeoffs improve regulatory impact analyses for electricity generation
Authors: Gingerich Daniel B. , Sun Xiaodi , Behrer A. Patrick , Azevedo Inês L. , Mauter Meagan S. 
Published: February 2017

SignificanceThe human health, environmental, and climate change implications of regulations affecting the electric power generation sector are typically assessed at the national scale. Variability in power plant emissions factors, regional grid composition, and location-specific marginal damages may lead to significant regional differences in the net benefits of regulations, including the recently promulgated Effluent Limitation Guidelines. Regional variation is further exacerbated by differences in the marginal damages of air, water, and solids emissions. Minimizing the externalities of electricity generation and reducing the compliance costs of emissions control require future regulatory design to use spatially resolved estimates of marginal damages and address air, water, and solids emissions control processes simultaneously.
HY5, a positive regulator of light signaling, negatively controls the unfolded protein response inArabidopsis
Authors: Nawkar Ganesh M. , Kang Chang Ho , Maibam Punyakishore , Park Joung Hun , Jung Young Jun , Chae Ho Byoung , Chi Yong Hun , Jung In Jung , Kim Woe Yeon , Yun Dae-Jin , Lee Sang Yeol 
Published: February 2017

SignificanceIn nature, plants are inevitably exposed to adverse conditions such as salinity, drought, and extreme temperatures. Recent reports suggest that environmental stresses critically affect the protein-folding capacity of endoplasmic reticula (ER), leading to ER stress. As the growth and development of plants significantly depend on light environment, the crosstalk between light signaling and ER stress response explained in current research can be a unique feature of plants. Our results suggest that light increases the ER stress sensitivity of plants and ELONGATED HYPOCOTYL 5, a positive regulator of light signaling, negatively regulates unfolded protein response gene expression in plant cells, which decreases the protein-folding capacity. The present study may form the basis for designing new strategies to increase stress tolerance of plants by tightly controlling light environment.
Molecular basis of fatty acid selectivity in the zDHHC family of S-acyltransferases revealed by click chemistry
Authors: Greaves Jennifer , Munro Kevin R. , Davidson Stuart C. , Riviere Matthieu , Wojno Justyna , Smith Terry K. , Tomkinson Nicholas C. O. , Chamberlain Luke H. 
Published: February 2017

Significance
S-acylation, the attachment of different fatty acids onto cysteine residues, regulates the activity of a diverse array of cellular proteins. This reversible posttranslational modification is essential for normal physiology, and defects are linked to human disease. S-acylation is catalyzed by a large family of zDHHC S-acyltransferases that use a cellular pool of diverse fatty acyl-CoAs as substrates. Using chemically synthesized probes, we show that individual zDHHC enzymes have marked differences in fatty acid selectivity and identify the underlying molecular basis for this property. This study identifies how acyl chain heterogeneity of S-acylated proteins is generated and is significant because the chemical nature of the attached S-acyl chain can fundamentally impact protein behavior.
Global socioeconomic material stocks rise 23-fold over the 20th century and require half of annual resource use
Authors: Krausmann Fridolin , Wiedenhofer Dominik , Lauk Christian , Haas Willi , Tanikawa Hiroki , Fishman Tomer , Miatto Alessio , Schandl Heinz , Haberl Helmut 
Published: February 2017

Significance
A large part of all primary materials extracted globally accumulates in stocks of manufactured capital, including in buildings, infrastructure, machinery, and equipment. These in-use stocks of materials provide important services for society and the economy and drive long-term demand for materials and energy. Configuration and quantity of stocks determine future waste flows and recycling potential and are key to closing material loops and reducing waste and emissions in a circular economy. A better understanding of in-use material stocks and their dynamics is essential for sustainable development. We present a comprehensive estimate of global in-use material stocks and of related material flows, including a full assessment of uncertainties for the 20th century as we analyze changes in stock-flow relations.
Phosphorylation of cardiac myosin binding protein C releases myosin heads from the surface of cardiac thick filaments
Authors: Kensler Robert W. , Craig Roger , Moss Richard L. 
Published: February 2017

Significance
Cardiac myosin binding protein C (cMyBP-C) is an important regulator of myocardial contraction, but its mechanism of action is unclear. In this study, we examined the structure of thick filaments from the hearts of mice in which the three serine residues that are phosphorylated by protein kinase A in the m-domain of cMyBP-C were replaced by either alanine or aspartic acid to mimic either the nonphosphorylated or phosphorylated state of cMyBP-C. In contrast to earlier work on rat cardiac filaments, the results support a model in which nonphosphorylated cMyBP-C stabilizes the relaxed/superrelaxed ordered “off-state” conformation of the heads while phosphorylation weakens the binding of the heads to the thick filament surface, increasing the probability of interaction with actin.
Impact of pre-Columbian “geoglyph” builders on Amazonian forests
Authors: Watling Jennifer , Iriarte José , Mayle Francis E. , Schaan Denise , Pessenda Luiz C. R. , Loader Neil J. , Street-Perrott F. Alayne , Dickau Ruth E. , Damasceno Antonia , Ranzi Alceu 
Published: February 2017

Significance
Amazonian rainforests once thought to be pristine wildernesses are increasingly known to have been inhabited by large populations before European contact. How and to what extent these societies impacted their landscape through deforestation and forest management is still controversial, particularly in the vast interfluvial uplands that have been little studied. In Brazil, the groundbreaking discovery of hundreds of geometric earthworks by modern deforestation would seem to imply that this region was also deforested to a large extent in the past, challenging the apparent vulnerability of Amazonian forests to human land use. We reconstructed environmental evidence from the geoglyph region and found that earthworks were built within man-made forests that had been previously managed for millennia. In contrast, long-term, regional-scale deforestation is strictly a modern phenomenon.
Estrogen receptor α wields treatment-specific enhancers between morphologically similar endometrial tumors
Authors: Droog Marjolein , Nevedomskaya Ekaterina , Dackus Gwen M. , Fles Renske , Kim Yongsoo , Hollema Harry , Mourits Marian J. , Nederlof Petra M. , van Boven Hester H. , Linn Sabine C. , van Leeuwen Flora E. , Wessels Lodewyk F. A. , Zwart Wilbert 
Published: February 2017

Significance
This study shows that the hormonal environment in which a tumor originates may affect a hormone receptor’s enhancer usage. We further show that enhancer function is less tissue specific than previously thought. By implementing ChIP sequencing in a unique patient cohort, we compared estrogen receptor α (ERα) profiles in endometrial tumors that developed in different hormonal environments and integrated these comparisons with transcriptomic data. Our data show that tumors associated with therapeutic intervention have a distinct ERα DNA-binding signature with regulatory potentials that resemble ERα-binding patterns in breast cancer. These results highlight the value of cistromic analyses in clinical specimens, which enabled us to distinguish subtypes of tumors on the level of transcriptional regulation.
Low HesperianPCO2constrained from in situ mineralogical analysis at Gale Crater, Mars
Authors: Bristow Thomas F. , Haberle Robert M. , Blake David F. , Des Marais David J. , Eigenbrode Jennifer L. , Fairén Alberto G. , Grotzinger John P. , Stack Kathryn M. , Mischna Michael A. , Rampe Elizabeth B. , Siebach Kirsten L. , Sutter Brad , Vaniman David T. , Vasavada Ashwin R. 
Published: February 2017

SignificanceApproximately 3.5-Ga sedimentary rocks surveyed by the Mars Science Laboratory rover in Gale Crater, Mars, contain secondary mineral phases indicating aqueous alteration and release of cations from mafic minerals during sediment deposition in lakes. However, carbonate phases are not detected, and our model calculations indicate atmospheric CO2levels at the time of sediment deposition 10s to 100s of times lower than those required by climate models to warm early Mars enough to maintain surficial water. Our results offer a ground-based reference point for the evolution of martian atmospheric CO2and imply that other mechanisms of warming Hesperian Mars, or processes that allowed for confined hydrological activity under cold conditions, must be sought.
Up-regulation of CD81 inhibits cytotrophoblast invasion and mediates maternal endothelial cell dysfunction in preeclampsia
Authors: Shen Li , Diao Zhenyu , Sun Hai-Xiang , Yan Gui-Jun , Wang Zhiqun , Li Ruo-Tian , Dai Yimin , Wang Jingmei , Li Jie , Ding Hailing , Zhao Guangfeng , Zheng Mingming , Xue Pingping , Liu Mo , Zhou Yan , Hu Yali 
Published: February 2017

Significance
Preeclampsia (PE) is a severe trophoblast-related disorder that threatens the health of mother and fetus. Impaired placentation in the early stages of pregnancy and the subsequent systemic endothelial cell activation constitute the principle pathogenic mechanisms of PE. Currently, the molecules involved in the pathogenesis of PE that link the two stages of this disorder remain elusive. Our study demonstrates that CD81 is associated with key pathological changes that occur in both the placenta and maternal endothelial cells of patients with severe PE (sPE). Importantly, overexpression of CD81 induces a PE-like phenotype in pregnant rats. This study provides evidence of the involvement of CD81 in the pathogenesis of PE and supports the use of CD81 as a potential biomarker for PE.