bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unlocking Enhanced Light Harvesting in Perovskites: A Light‐Mediated Ligand Management Approach
Authors: Singh Siddharth , Ganguly Debarjya , Aggarwal Pooja , Govind Rao Vishal 
Published: September 2024

AbstractInterfaces between nanocrystals and their stabilizing ligands in light‐harvesting devices are crucial for mediating energy transfer, essential for efficient solar energy conversion. In metal halide perovskites, a promising material for these devices, Förster resonance energy transfer (FRET) within perovskite/acceptor‐molecule complexes offers a pathway for optimized energy transfer. However, traditional methods for optimizing FRET in perovskite‐chromophore hybrids are based on static modifications. This study presents an innovative approach for light‐driven dynamic control of FRET, achieving an ≈14% enhancement in efficiency between CsPbBr3 nanocrystals (CPB NCs) and rhodamine B isothiocyanate (RITC) dye. UV light is utilized to reversibly regulate NC‐ligand binding and thus control the coupling between CPB NCs and RITC at their interface. This approach critically relies on strong NC–ligand interactions, such as the Pb─S bond between CPB NCs and RITC. Light exposure weakens the binding of surface ligands, allowing RITC, with its strong bond, to attach more effectively and promote enhanced energy transfer. This light‐activated control is absent with Rhodamine B (RhB) lacking ─NCS group, a strong binding motif. The findings reveal the importance of NC–ligand interactions in dynamically manipulating FRET with light. This pioneering method for nanoscale FRET control paves the way for more efficient light‐harvesting devices.
Ag‐Catalyzed Asymmetric 1,3‐Dipolar Cycloaddition of N‐2,2,2‐Trifluoroethylisatin Ketimines with Conjugated Exocyclic Double Bonds: Access to Pyrrolidines with Four Consecutive Stereocenters Including Two Adjacent Spiro‐Quaternary Centers
Authors: Liu Ji‐Hong , Zhang Yan‐Ping , Wang Zhen‐Hua , Lu Ji‐Hong , Yang Lei , You Yong , Zhao Jian‐Qiang , Zhou Ming‐Qiang , Yuan Wei‐Cheng 
Published: September 2024

AbstractAg‐catalyzed enantioselective 1,3‐dipolar cycloaddition of N‐2,2,2‐trifluoroethylisatin ketimines with conjugated exocyclic double bonds including α‐alkylidene succinimides and aurone derivatives was developed for the first time. Using a catalyst system consisting of a ferrocenyl P,N‐ligand and AgNO2, the reaction provides a facile method for the construction of chiral pyrrolidines bearing four consecutive stereocenters and two adjacent spiro‐quaternary chiral centers, as well as offers a direct route to structurally diverse enantioenriched CF3‐containing 3,2′‐pyrrolidinyl spirooxindole derivatives.
Spatiotemporal Analysis of Mesenchymal Stem Cells Fate Determination by Inflammatory Niche Following Soft Tissue Injury at a Single‐Cell Level
Authors: Kan Chen , Tan Zhenya , Wang Haitao , Wang Wei , Yang Jiazhao , Zhang Ya , Lu Xiaoling , Cheng Qirong , Chai Lanyi , Peng Chao , Zhu Jicheng , Zhu Chenghang , Wang Hailin , Zhan Li , Lin Keqiong , Liu Yakun , Zhang Lingqiang , Fan Haitao , Zheng Hong 
Published: September 2024

AbstractHeterotopic ossification (HO), often arising in response to traumatic challenges, results from the aberrant osteochondral differentiation of mesenchymal stem cells (MSCs). Nevertheless, the impact of trauma‐induced inflammatory exposure on MSC fate determination remains ambiguous. In this study, the cellular diversity within inflammatory lesions is elucidated, comprising MSCs and several innate and adaptive immune cells. It is observed that quiescent MSCs transition into cycling MSCs, subsequently giving rise to chondrogenic (cMSC) and/or osteogenic (oMSC) lineages within the inflammatory microenvironment following muscle or tendon injuries, as revealed through single‐cell RNA sequencing (scRNA‐seq), spatial transcriptome and lineage tracing analysis. Moreover, these investigations demonstrate that neutrophils and natural killer (NK) cells enhance transition of quiescent MSCs into cycling MSCs, which is also controlled by M1 macrophages, a subpopulation of macrophages can also stimulate cMSC and oMSC production from cycling MSCs. Additionally, M2 macrophages, CD4+ and CD8+ T lymphocytes are found to promote chondrogenesis. Further analysis demonstrates that immune cells promotes the activation of signaling transducers and activators of transcription (STAT) pathway and phosphoinositide 3 (PI3K)/protein kinase B (AKT) pathway in MSC proliferation and osteochondral progenitors’ production, respectively. These findings highlight the dynamics of MSC fate within the inflammatory lesion and unveil the molecular landscape of osteoimmunological interactions, which holds promise for advancing HO treatment.
A ROS‐Responsive Lipid Nanoparticles Release Multifunctional Hydrogel Based on Microenvironment Regulation Promotes Infected Diabetic Wound Healing
Authors: Yang Hao , Lv Dongming , Qu Shanqiang , Xu Hailin , Li Shuting , Wang Zhiyong , Cao Xiaoling , Rong Yanchao , Li Xiaohui , Wu Honglin , Chen Yongfei , Zhu Jiayuan , Tang Bing , Hu Zhicheng 
Published: September 2024

AbstractThe continuous imbalance of the diabetic wound microenvironment is an important cause of chronic nonhealing, which manifests as a vicious cycle between excessive accumulation of reactive oxygen species (ROS) and abnormal healing. Regulating the microenvironment by suppressing wound inflammation, oxidative stress, and bacterial infection is a key challenge in treating diabetic wounds. In this study, ROS‐responsive hydrogels are developed composed of silk fibroin methacrylated (SFMA), modified collagen type III (rCol3MA), and lipid nanoparticles (LNPs). The newly designed hydrogel system demonstrated stable physicochemical properties and excellent biocompatibility. Moreover, the release of antimicrobial peptide (AMP) and puerarin (PUE) demonstrated remarkable efficacy in eradicating bacteria, regulating inflammatory responses, and modulating vascular functions. This multifunctional hydrogel is a simple and efficient approach for the treatment of chronic diabetic infected wounds and holds tremendous potential for future clinical applications.
Evolution and Domestication of a Novel Biosynthetic Gene Cluster Contributing to the Flavonoid Metabolism and High‐Altitude Adaptability of Plants in the Fagopyrum Genus
Authors: Huang Xu , He Yuqi , Zhang Kaixuan , Shi Yaliang , Zhao Hui , Lai Dili , Lin Hao , Wang Xiangru , Yang Zhimin , Xiao Yawen , Li Wei , Ouyang Yinan , Woo Sun Hee , Quinet Muriel , Georgiev Milen I. , Fernie Alisdair R. , Liu Xu , Zhou Meiliang 
Published: September 2024

AbstractThe diversity of secondary metabolites is an important means for plants to cope with the complex and ever‐changing terrestrial environment. Plant biosynthetic gene clusters (BGCs) are crucial for the biosynthesis of secondary metabolites. The domestication and evolution of BGCs and how they affect plant secondary metabolites biosynthesis and environmental adaptation are still not fully understood. Buckwheat exhibits strong resistance and abundant secondary metabolites, especially flavonoids, allowing it to thrive in harsh environments. A non‐canonical BGC named UFGT3 cluster is identified, which comprises a phosphorylase kinase (PAK), two transcription factors (MADS1/2), and a glycosyltransferase (UFGT3), forming a complete molecular regulatory module involved in flavonoid biosynthesis. This cluster is selected during Tartary buckwheat domestication and is widely present in species of the Fagopyrum genus. In wild relatives of cultivated buckwheat, a gene encoding anthocyanin glycosyltransferase (AGT), which glycosylates pelargonidin into pelargonidin‐3‐O‐glucoside, is found inserted into this cluster. The pelargonidin‐3‐O‐glucoside can help plants resist UV stress, endowing wild relatives with stronger high‐altitude adaptability. This study provides a new research paradigm for the evolutionary dynamics of plant BGCs, and offers new perspectives for exploring the mechanism of plant ecological adaptability driven by environmental stress through the synthesis of secondary metabolites.
Multi‐Zone Visco‐Node‐Pore Sensing: A Microfluidic Platform for Multi‐Frequency Viscoelastic Phenotyping of Single Cells
Authors: Lai Andre , Hinz Stefan , Dong Alan , Lustig Michael , LaBarge Mark A. , Sohn Lydia L. 
Published: September 2024

AbstractThis study introduces multi‐zone visco‐Node‐Pore Sensing (mz‐visco‐NPS), an electronic‐based microfluidic platform for single‐cell viscoelastic phenotyping. mz‐visco‐NPS implements a series of sinusoidal‐shaped contraction zones that periodically deform a cell at specific strain frequencies, leading to changes in resistance across the zones that correspond to the cell's frequency‐dependent elastic G′ and viscous G″ moduli. mz‐visco‐NPS is validated by measuring the viscoelastic changes of MCF‐7 cells when their cytoskeleton is disrupted. mz‐visco‐NPS is also employed to measure the viscoelastic properties of human mammary epithelial cells across the entire continuum of epithelial transformation states, from average‐ and high‐risk primary epithelial cells, to immortal non‐malignant (MCF‐10A), malignant (MCF‐7), and metastatic (MDA‐MB‐231) cell lines. With a throughput of 600 cells per hour and demonstrated ease‐of‐use, mz‐visco‐NPS reveals a remarkable level of single‐cell heterogeneity that would otherwise be masked by ensemble averaging.
Nucleic Acid Amplification Circuit‐Based Hydrogel (NACH) Assay for One‐Step Detection of Metastatic Gastric Cancer‐Derived Exosomal miRNA
Authors: Seo Seung Beom , Lim Jaewoo , Kim Kyujung , Maeng Inhee , Rho Hyun Wook , Son Hye Young , Kim Eunjung , Jang Eunji , Kang Taejoon , Jung Juyeon , Oh Seung Jae , Huh Yong‐Min , Lim Eun‐Kyung 
Published: September 2024

AbstractGastric cancer (GC) is recognized as the fifth most prevalent malignant tumor worldwide. It is characterized by diverse clinical symptoms, treatment responses, and prognoses. In GC prognosis, the promotion of epithelial–mesenchymal transition (EMT) fosters cancer cell invasion and metastasis, thereby triggering the dissemination of tumor cells. This study proposes a nucleic acid amplification circuit‐based hydrogel (NACH) assay for identifying exosomal miRNA derived from metastatic GC. The NACH assay employs the rolling circle amplification method and targets miRNA‐21, a tumor‐related oncogene, and miRNA‐99a, which promotes EMT. Specific amplification probes for each target are immobilized within the hydrogel, enabling a streamlined, one‐step amplification reaction. The NACH assay exhibits a detection limit of 1 fm for miRNA‐21 and miRNA‐99a, thereby enabling rapid and highly sensitive on‐site detection. Performance evaluation using exosomal miRNA extracted from cell culture media, mouse plasma, and human plasma revealed fluorescence intensity patterns similar to those obtained in qRT‐PCR. Furthermore, deploying a custom‐developed portable fluorometer for the NACH assay allows for diagnostic performance assessment and point‐of‐care testing using clinical samples from GC patients. These findings emphasize the potential of the NACH assay to be used as a robust tool for the genetic diagnosis of GC based on exosome detection.
A Three–Dimensional Nanoscale View of Electrocatalyst Degradation in Hydrogen Fuel Cells
Authors: Amichi Lynda , Yu Haoran , Ziabari Amirkoushyar , Rahman Obaidullah , Arregui‐Mena David , Hu Leiming , Neyerlin K. C. , Cullen David A. 
Published: September 2024

AbstractThe loss of platinum (Pt) electrochemically active surface area (ECSA) is a critical degradation mode that often becomes a limiting factor for heavy‐duty proton exchange membrane fuel cell vehicles. High surface area carbon supports have been shown to improve Pt dispersion and limit detrimental ionomer‐electrocatalyst interactions due to their large interior pore volume. In this work, using automated scanning transmission electron tomography, the degradation of nanoparticles located on the interior versus exterior surfaces of the carbon support is compared following a catalyst‐specific accelerated stress test (AST) of 90,000 voltage cycles between 0.6 V to 0.95 V. The results reveal a notable increase in median particle size for both interior and exterior Pt catalyst particles, with a slightly higher increase in particle size distribution and loss of specific surface area for the particles located on the exterior carbon surface. The fraction of Pt nanoparticles that reside within the interior of the carbon support also increased following the AST test, accompanied by evidence of an increase in average carbon mesopore size. The results shed light on the degradation mechanisms affecting electrochemical properties and the enhanced particle accessibility at lower relative humidity.
Molecular layer deposition of polyhydroquinone thin films for Li‐ion battery applications
Authors: Paranamana Nikhila C. , Datta Amit K. , Wyatt Quinton K. , Gettler Ryan C. , Werbrouck Andreas , Young Matthias J. 
Published: September 2024

AbstractMany next‐generation materials for Li‐ion batteries are limited by material instabilities. To stabilize these materials, ultrathin, protective coatings are needed that conduct both lithium ions and electrons. Here, we demonstrate a hybrid chemistry combining molecular layer deposition (MLD) of trimethylaluminum (TMA) and p‐hydroquinone (HQ) with oxidative molecular layer deposition (oMLD) of molybdenum pentachloride (MoCl5) and HQ to enable vapor‐phase molecular layer growth of poly(p‐hydroquinone) (PHQ)—a mixed electron and lithium ion conducting polymer. We employ quartz crystal microbalance (QCM) studies to understand the chemical mechanism and demonstrate controlled linear growth with a 0.5 nm/cycle growth rate. Spectroscopic characterization indicates that this hybrid MLD/oMLD chemistry polymerizes surface HQ monomers from the TMA‐HQ chemistry to produce PHQ. The polymerization to PHQ improves air stability over MLD TMA‐HQ films without crosslinking. Electrochemical measurements on hybrid MLD/oMLD films indicate electronic conductivity of ~10−9 S/cm and a Li‐ion conductivity of ~10−4 S/cm. While these coatings show promise for Li‐ion battery applications, this work focuses on establishing the coating chemistry and future studies are needed to examine the stability, structure, and cycling performance of these coatings in full Li‐ion cells.
Cracking the physical insight of power law models: Bridging the gap between macroscopic kinetics and surface coverages
Authors: Vega‐Ramon Fernando , Rogers Alexander W. , Hardacre Christopher , Zhang Dongda 
Published: September 2024

AbstractWe propose a methodological framework to quantify the relative abundance of key surface intermediates via analysis of the macroscopic intrinsic kinetic characteristics of gas‐phase data. At the core of this approach is the development of analytical expressions, which link reaction orders and activation energies in macroscopic power law models to the fractional coverage of the non‐observable intermediates. Through the design of an advanced parameter estimation methodology, these relationships were further exploited to develop thermodynamically consistent and mechanistic‐based power law models which could capture the evolution of surface occupancy profiles along the reactor length. The developed framework was tested through in‐silico water gas shift reaction case‐studies; the results indicate the robustness of the approach and the mechanistic insight that can be gained from its application. The methodology proposed in this study could thus be of general value to assist in reaction mechanism analysis, identification of key surface intermediates, and reactor optimization.
Howler Monkey Die‐Off in Southern Mexico
Authors: Pozo‐Montuy Gilberto , Aguilar‐Cucurachi María del Socorro , Aureli Filippo , Briseño‐Jaramillo Margarita , Canales‐Espinosa Domingo , Cárdenas‐Navarrete Anaid , Cortés‐Ortiz Liliana , Coyohua‐Fuentes Alejandro , Espinosa‐Gómez Fabiola Carolina , Franquesa‐Soler Montserrat , García‐Duran Candelaria , García‐Ojeda Yuliana , González‐Acosta Mónica Rosario , Hermida‐Lagunes Javier , Hernández‐Salazar Laura Teresa , Jasso‐del‐Toro Cristina , Lizama‐Hernández José Alberto , Martínez‐Ramos Ileana Zorhaya , Montejo‐Zetina Edgar J. , Núñez‐Martínez Guadalupe , Nuñez‐Ramírez Paulina Y. , Pareja‐Badillo Pedro Shautamai , Pinacho‐Guendulain Braulio , Ramos‐Fernández Gabriel , Rangel‐Negrín Ariadna , Rivera‐Sánchez Alix Fernanda , Sánchez‐Domínguez Elizabeth , Serio‐Silva Juan Carlos , Smith‐Aguilar Sandra E. , Solórzano‐García Brenda , Spaan Denise , Van Belle Sarie , Dias Pedro A. D. 
Published: September 2024

ABSTRACTIn May and June 2024, a die‐off of Mexican mantled howler monkeys (Alouatta palliata mexicana) occurred in southern Mexico. This commentary documents the event, attributing it to extreme heatwaves, drought, wildfires, and habitat impoverishment. Despite their reported resilience to habitat disturbances, mantled howler monkey mortality rate in some areas reached 31%. Key evidence points to heatstroke as the primary cause of death, exacerbated by limited hydration and reduced dietary diversity in disturbed habitats. Immediate responses included community‐led rescues (e.g., hydrating the monkeys), coordination of rescue activities by nongovernmental organizations (NGOs) (e.g., managing donations), involvement of scientists (e.g., monitoring of primate populations), and assistance from government officials (e.g., providing legal support for animal management). This event underscores the urgency of developing action plans to prevent and attend future crises. Among other actions, we highlight (i) establishing primate care infrastructure with medical and rehabilitation centers; (ii) developing protocols and training programs to ensure rapid crisis response; (iii) fostering collaboration among government, NGOs, and academic institutions for effective crisis management; and (iv) developing targeted research on climate change impacts, predictive models, and long‐term health monitoring. We emphasize the critical need for coordinated conservation efforts to protect wild primates and maintain natural ecosystem resilience in the face of escalating climate challenges.
Loss of TREM2 diminishes CAA despite an overall increase of amyloid load in Tg‐SwDI mice
Authors: Zhong Rui , Xu Yingzheng , Williams Jesse W. , Li Ling 
Published: September 2024

AbstractINTRODUCTIONThe microglial receptor triggering receptor expressed on myeloid cells 2 (TREM2) is a major risk factor for Alzheimer's disease (AD). Experimentally, Trem2 deficiency affects parenchymal amyloid beta (Aβ) deposition. However, the role of TREM2 in cerebrovascular amyloidosis, especially cerebral amyloid angiopathy (CAA), remains unexplored.METHODSTg‐SwDI (SwDI) mice, a CAA‐prone model of AD, and Trem2 knockout mice were crossed to generate SwDI/TWT, SwDI/THet, and SwDI/TKO mice, followed by pathological and biochemical analyses at 16 months of age.RESULTSLoss of Trem2 led to a dramatic decrease in CAA and microglial association, despite a marked increase in overall brain Aβ load. Single nucleus RNA sequencing analysis revealed that in the absence of Trem2, microglia were activated but trapped in transition to the fully reactive state, with distinct responses of vascular cells.DISCUSSIONOur study provides the first evidence that TREM2 differentially modulates parenchymal and vascular Aβ pathologies, offering significant implications for both TREM2‐ and Aβ‐targeting therapies for AD.Highlights
Triggering receptor expressed on myeloid cells 2 (TREM2) differentially modulates brain parenchymal and vascular amyloidosis.
Loss of Trem2 markedly reduces cerebral amyloid angiopathy despite an overall increase of amyloid beta load in Tg‐SwDI mice.
Microglia are trapped in transition to the fully reactive state without Trem2.
Perivascular macrophages and other vascular cells have distinct responses to Trem2 deficiency.
Balanced TREM2‐targeting therapies may be required for optimal outcomes.
Self‐ and study partner–reported cognitive decline in older adults without dementia: The role of α‐synuclein and amyloid biomarkers in the Alzheimer's Disease Neuroimaging Initiative
Authors: Thomas Kelsey R. , Bangen Katherine J. , Rotblatt Lindsay J. , Weigand Alexandra J. , Edwards Lauren , Tosun Duygu , Galasko Douglas ,   for the Alzheimer's Disease Neuroimaging Initiative
Published: September 2024

AbstractINTRODUCTIONSubjective cognitive decline (SCD) may be an early marker of Alzheimer's disease (AD) pathology. Until recently, it was impossible to measure biomarkers specific for α‐synuclein pathology; therefore, its association with subjective reports of cognitive decline is unknown.METHODSAlzheimer's Disease Neuroimaging Initiative participants without dementia (n = 918) were classified as positive or negative for amyloid beta (Aβ+ or Aβ−) and α‐synuclein (α‐syn+ or α‐syn−) biomarkers. Self‐ and study partner–reported cognitive decline was measured with the Everyday Cognition (ECog) questionnaire.RESULTSPer self‐report, Aβ+/α‐syn+ had the greatest cognitive decline. Aβ−/α‐syn+ did not differ from Aβ−/α‐syn− across ECog scores. Study partner–reported results had a similar pattern, but Aβ+/α‐syn− and Aβ+/α‐syn+ did not differ across ECog scores. Mild cognitive impairment classification moderated the study partner–reported memory score.DISCUSSIONWhile α‐syn+ alone did not increase subjective reports of cognitive decline, Aβ+/α‐syn+ had the most self‐ and study partner–rated cognitive decline. Therefore, the presence of multiple pathologies was associated with greater SCD.Highlights

Cerebrospinal fluid α‐synuclein (α‐syn) seed amplification assay was used to determine α‐syn positivity.
Amyloid beta (Aβ)−/α‐syn−, Aβ−/α‐syn+, Aβ+/α‐syn−, and Aβ+/α‐syn+ biomarker groups were created.
Aβ+/α‐syn+ had greater subjective cognitive decline (SCD) than the other biomarker groups.
Aβ−/α‐syn+ did not differ from Aβ−/α‐syn− across self‐ or study–partner reported SCD scores.
Study partner–reported subjective memory results were largely driven by participants with mild cognitive impairment.
Atmosphere Induces Tunable Oxygen Vacancies to Stabilize Single‐Atom Copper in Ceria for Robust Electrocatalytic CO2 Reduction to CH4
Authors: Huang Fang , Chen Xiangyu , Sun Huanhuan , Zeng Qingduo , Ma Junjie , Wei Dong , Zhu Jinliang , Chen Zhengjun , Liang Taoyuan , Yin Xucai , Liu Xijun , Xu Jing , He Huibing 
Published: September 2024

AbstractElectrochemical carbon dioxide reduction (ECO2RR) shows great potential to create high‐value carbon‐based chemicals, while designing advanced catalysts at the atomic level remains challenging. The ECO2RR performance is largely dependent on the catalyst microelectronic structure that can be effectively modulated through surface defect engineering. Here, we provide an atmosphere‐assisted low‐temperature calcination strategy to prepare a series of single‐atomic Cu/ceria catalysts with varied oxygen vacancy concentrations for robust electrolytic reduction of CO2 to methane. The obtained Cu/ceria catalyst under H2 environment (Cu/ceria‐H2) exhibits a methane Faraday efficiency (FECH4) of 70.03 % with a turnover frequency (TOFCH4) of 9946.7 h−1 at an industrial‐scale current density of 150 mA cm−2 in a flow cell. Detailed studies indicate the copious oxygen vacancies in the Cu/ceria‐H2 are conducive to regulating the surface microelectronic structure with stabilized Cu+ active center. Furthermore, density functional theory calculations and operando ATR‐SEIRAS demonstrate that the Cu/ceria‐H2 can markedly enhance the activation of CO2, facilitate the adsorption of pivotal intermediates *COOH and *CO, thus ultimately enabling the high selectivity for CH4 production. This study presents deep insights into designing effective electrocatalysts for CO2 to CH4 conversion by controlling the surface microstructure via the reaction atmosphere.