bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cooperative function of Fmp30, Mdm31, and Mdm32 in Ups1-independent cardiolipin accumulation in the yeast Saccharomyces cerevisiae
Authors: Miyata Non , Goda Naoto , Matsuo Keiji , Hoketsu Takeshi , Kuge Osamu 
Published: November 2017

AbstractCardiolipin (CL) is synthesized from phosphatidic acid (PA) through a series of enzymatic reactions occurring at the mitochondrial inner membrane (MIM). Ups1-Mdm35 mediates PA transfer from the mitochondrial outer membrane (MOM) to the MIM in the yeast Saccharomyces cerevisiae. Deletion of UPS1 leads to a ~80% decrease in the cellular CL level. However, the CL accumulation in ups1∆ cells is enhanced by the depletion of Ups2, which forms a protein complex with Mdm35 and mediates phosphatidylserine (PS) transfer from the MOM to the MIM for phosphatidylethanolamine (PE) synthesis by a PS decarboxylase, Psd1. In this study, we found that the accumulation of CL in ups1∆ cells was enhanced by deletion of not only UPS2, but also PSD1 and CHO1 encoding a PS synthase, suggesting that low PE levels in mitochondria were relevant to the enhancement of CL accumulation in ups1∆ cells. Furthermore, the Ups1-independent and low-level PE-enhanced CL accumulation was shown to depend on the functions of FMP30, MDM31, and MDM32. In addition, the physical interactions of Fmp30 with Mdm31 and Mdm32 were revealed. Thus, when the mitochondrial PE level is reduced, Fmp30, Mdm31, and Mdm32 seem to function cooperatively for the accumulation of CL in a UPS1-independent manner.
Electronic Health Record Driven Prediction for Gestational Diabetes Mellitus in Early Pregnancy
Authors: Qiu Hang , Yu Hai-Yan , Wang Li-Ya , Yao Qiang , Wu Si-Nan , Yin Can , Fu Bo , Zhu Xiao-Juan , Zhang Yan-Long , Xing Yong , Deng Jun , Yang Hao , Lei Shun-Dong 
Published: November 2017

AbstractGestational diabetes mellitus (GDM) is conventionally confirmed with oral glucose tolerance test (OGTT) in 24 to 28 weeks of gestation, but it is still uncertain whether it can be predicted with secondary use of electronic health records (EHRs) in early pregnancy. To this purpose, the cost-sensitive hybrid model (CSHM) and five conventional machine learning methods are used to construct the predictive models, capturing the future risks of GDM in the temporally aggregated EHRs. The experimental data sources from a nested case-control study cohort, containing 33,935 gestational women in West China Second Hospital. After data cleaning, 4,378 cases and 50 attributes are stored and collected for the data set. Through selecting the most feasible method, the cost parameter of CSHM is adapted to deal with imbalance of the dataset. In the experiment, 3940 samples are used for training and the rest 438 samples for testing. Although the accuracy of positive samples is barely acceptable (62.16%), the results suggest that the vast majority (98.4%) of those predicted positive instances are real positives. To our knowledge, this is the first study to apply machine learning models with EHRs to predict GDM, which will facilitate personalized medicine in maternal health management in the future.
Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus
Authors: Sakakibara Shuhei , Arimori Takao , Yamashita Kazuo , Jinzai Hideyuki , Motooka Daisuke , Nakamura Shota , Li Songling , Takeda Kazuya , Katayama Jun , El Hussien Marwa Ali , Narazaki Masashi , Tanaka Toshio , Standley Daron M. , Takagi Junichi , Kikutani Hitoshi 
Published: November 2017

AbstractThe evolutional process of disease-associated autoantibodies in systemic lupus erythematosus (SLE) remains to be established. Here we show intraclonal diversification and affinity maturation of anti-nuclear antibody (ANA)-producing B cells in SLE. We identified a panel of monoclonal ANAs recognizing nuclear antigens, such as double-stranded DNA (dsDNA) and ribonucleoproteins (RNPs) from acute SLE subjects. These ANAs had relatively few, but nonetheless critical mutations. High-throughput immunoglobulin sequencing of blood lymphocytes disclosed the existence of sizable ANA lineages shearing critical mutations intraclonally. We further focused on anti-DNA antibodies, which are capable to bind to both single-stranded (ss) and dsDNA at high affinity. Crystal structure and biochemical analysis confirmed a direct role of the mutations in the acquisition of DNA reactivity and also revealed that these anti-DNA antibodies recognized an unpaired region within DNA duplex. Our study unveils the unique properties of high-affinity anti-DNA antibodies that are generated through antigen-driven affinity maturation in acute phase of SLE.
Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics
Authors: Boufaied Nadia , Nash Claire , Rochette Annie , Smith Anthony , Orr Brigid , Grace O. Cathal , Wang Yu Chang , Badescu Dunarel , Ragoussis Jiannis , Thomson Axel A. 
Published: November 2017

AbstractProstate organogenesis involves epithelial growth controlled by inductive signalling from specialised mesenchymal subsets. To identify pathways active in mesenchyme we used tissue and single cell transcriptomics to define mesenchymal subsets and subset-specific transcript expression. We documented transcript expression using Tag-seq and RNA-seq in female rat Ventral Mesenchymal Pad (VMP) as well as adjacent urethra comprised of smooth muscle and peri-urethral mesenchyme. Transcripts enriched in female VMP were identified with Tag-seq of microdissected tissue, RNA-seq of cell populations, and single cells. We identified 400 transcripts as enriched in the VMP using bio-informatic comparisons of Tag-seq and RNA-seq data, and 44 were confirmed by single cell RNA-seq. Cell subset analysis showed that VMP and adjacent mesenchyme were composed of distinct cell types and that each tissue contained two subgroups. Markers for these subgroups were highly subset specific. Thirteen transcripts were validated by qPCR to confirm cell specific expression in microdissected tissues, as well as expression in neonatal prostate. Immunohistochemical staining demonstrated that Ebf3 and Meis2 showed a restricted expression pattern in female VMP and prostate mesenchyme. We conclude that prostate inductive mesenchyme shows limited cellular heterogeneity and that transcriptomic analysis identified new mesenchymal subset transcripts associated with prostate organogenesis.
A PLGA-reinforced PEG in situ gel formulation for improved sustainability of hypoglycaemic activity of glimepiride in streptozotocin-induced diabetic rats
Authors: Ahmed Osama A. A. , El-Say Khalid M. , Alahdal Abdulrahman M. 
Published: November 2017

AbstractGlimepiride (GMD) is a third-generation sulfonylurea derivative and one of the top three most-prescribed oral antidiabetic drugs. The need for a depot formulation exists, and a safe and effective antidiabetic therapy is the goal of this study. The aims were to design a depot in situ gel (ISG) formulation and investigate the main factors that control the initial burst and sustain the GMD effect using the Box-Behnken design. The studied factors were polymer percent (X1), plasticizer percent (X2) and benzyl benzoate percent in N-methyl-2-pyrrolidone (X3). The results revealed that X2 is the only factor that showed significant effects on all investigated responses. Scanning electron microscopy images showed that an increase in PEG % improved the smoothness and reduced the porosity of the ISG formulation surface. The GMD plasma levels in diabetic rats revealed no significant difference (p < 0.05) between the maximum GMD plasma concentrations of the optimized GMD-ISG formula (10 mg/ kg) and oral marketed GMD tablets (1 mg/kg). This result ensures that the optimized formula does not exceed the maximum safe plasma concentration. In addition, the optimized GMD-ISG formulation showed a depot effect that lasted for 14 days post-injection. This approach to controlling GMD release using an in situ forming system could be useful for improving patient compliance and diabetes treatment effectiveness.
Preparation of ZnS@In2S3 Core@shell Composite for Enhanced Photocatalytic Degradation of Gaseous o-Dichlorobenzene under Visible Light
Authors: Liu Baojun , Hu Xia , Li Xinyong , Li Ying , Chen Chang , Lam Kwok-ho 
Published: November 2017

AbstractIn this study, novel ZnS@In2S3 core@shell hollow nanospheres were fabricated by a facile refluxing method for the first time, and the formation mechanism of hollow structure with interior architecture was discussed based on ion-exchange Ostwald ripening. As the photocatalytic material for degradation of gaseous o-Dichlorobenzene (o-DCB), the as-synthesized core@shell hollow nanospheres were found to show significantly enhanced catalytic performance for effective separation of photo-generated charges. Moreover, the mechanisms of enhanced activity were elucidated by band alignment and unique configuration. Such photocatalyst would meet the demands for the control of persistent organic pollutant (POPs) in the atmospheric environment.
Infalling groups and galaxy transformations in the cluster A2142
Authors: Einasto Maret , Deshev Boris , Lietzen Heidi , Kipper Rain , Tempel Elmo , Park Changbom , Gramann Mirt , Heinämäki Pekka , Saar Enn , Einasto Jaan 
Published: November 2017

Context.Superclusters of galaxies provide dynamical environments for the study of the formation and evolution of structures in the cosmic web from galaxies, to the richest galaxy clusters, and superclusters themselves.Aims.We study galaxy populations and search for possible merging substructures in the rich galaxy cluster A2142 in the collapsing core of the supercluster SCl A2142, which may give rise to radio and X-ray structures in the cluster, and affect galaxy properties of this cluster.Methods.We used normal mixture modelling to select substructure of the cluster A2142. We compared alignments of the cluster, its brightest galaxies (hereafter BCGs), subclusters, and supercluster axes. The projected phase space (PPS) diagram and clustercentric distributions are used to analyse the dynamics of the cluster and study the distribution of various galaxy populations in the cluster and subclusters.Results.We find several infalling galaxy groups and subclusters. The cluster, supercluster, BCGs, and one infalling subcluster are all aligned. Their orientation is correlated with the alignment of the radio and X-ray haloes of the cluster. Galaxy populations in the main cluster and in the outskirts subclusters are different. Galaxies in the centre of the main cluster at the clustercentric distances 0.5 h−1Mpc (Dc∕Rvir< 0.5,Rvir= 0.9 h−1Mpc) have older stellar populations (with the median age of 10−11 Gyr) than galaxies at larger clustercentric distances. Star-forming and recently quenched galaxies are located mostly at the clustercentric distancesDc≈ 1.8 h−1Mpc, where subclusters fall into the cluster and the properties of galaxies change rapidly. In this region the median age of stellar populations of galaxies is about 2 Gyr. Galaxies in A2142 on average have higher stellar masses, lower star formation rates, and redder colours than galaxies in rich groups. The total mass in infalling groups and subclusters isM≈ 6 × 1014h−1M⊙, that is approximately half of the mass of the cluster. This mass is sufficient for the mass growth of the cluster from redshiftz= 0.5 (half-mass epoch) to the present.Conclusions.Our analysis suggests that the cluster A2142 has formed as a result of past and present mergers and infallen groups, predominantly along the supercluster axis. Mergers cause complex radio and X-ray structure of the cluster and affect the properties of galaxies in the cluster, especially at the boundaries of the cluster in the infall region. Explaining the differences between galaxy populations, mass, and richness of A2142, and other groups and clusters may lead to better insight about the formation and evolution of rich galaxy clusters.
Wenn der Puls rast
Authors: Reuter Janine 
Published: November 2017

Zusammenfassung
Interventionskoffer, die Elfte Erinnern Sie sich noch an Ihre erste eskalierende Situation auf Station? Wie haben Sie sich dabei
gefühlt? Haben Sie gehofft, dass dieser Moment schnell vorbeigeht? Ähnlich geht es Auszubildenden, die zum ersten Mal mit Akutsituationen
konfrontiert werden. Wie Sie deren Ängste auffangen und sie Schritt für Schritt an den Umgang mit selbst- oder fremdgefährdenden Patienten
heranführen, schildert unsere Autorin anhand eines Fallbeispiels.
Alle Sinne bewegen
Authors: Neumann Julia , Rudolph Jule Marie 
Published: November 2017

Zusammenfassung
Demenz Kochen, Backen und Gärtnern berühren eine Vielzahl der Sinne und lösen bei vielen Menschen das Gefühl aus, zu Hause zu sein und
sich wohlzufühlen. Auch Demenzerkrankte sind häufig noch in der Lage, diese Tätigkeiten – unter pflegerischer Anleitung – auszuführen. Ob es
gelingt, ihnen dadurch ein Stück Lebensqualität zurückzugeben, haben unsere Autorinnen in einer systematischen Literaturrecherche
untersucht.
Aufbruch in eine neue Welt
Authors: Wagner-Stramke Christina 
Published: November 2017

Zusammenfassung
Bericht einer Zeitzeugin Fast 50 Jahre hat unsere Autorin in der psychiatrischen Pflege gearbeitet. In ihrem sehr persönlichen
Rückblick erzählt sie von den Missständen der Verwahrpsychiatrie in den 1960er-Jahren und erläutert, warum die Psychiatriereform kommen
musste und die sozialpsychiatrische Weiterbildung für sie der Aufbruch in eine neue Welt war.
The Jellyfish Sign: A New Sonographic Cervical Marker to Predict Maternal Morbidity in Abnormally Invasive Placenta Previa
Authors: Bertucci Emma , Sileo Filomena , Grandi Giovanni , Fenu Valentina , Cani Carlotta , Mancini Luciano , Mataca Ema , Facchinetti Fabio 
Published: November 2017

Abstract
Purpose To investigate the value of a new cervical sonographic sign, called the jellyfish sign (JS), for predicting the risk of maternal morbidity in cases of abnormally invasive placenta (AIP) previa totalis.
Materials and Methods Retrospective evaluation of transvaginal (TV) and transabdominal (TA) scans performed in all singleton pregnancies with placenta previa totalis. JS, i. e. the absence of the normal linear demarcation between the placenta previa and the cervix, was evaluated by TV scans. The presence/severity of AIP and outcomes of maternal morbidity were related to this sign.
Results JS was noted in 8/39 (20.5 %) patients. The two analyzed groups, i. e. with and without JS, were similar. The specificity of JS in AIP diagnosis, histological findings of accreta/increta/percreta, need for caesarean hysterectomy or blood loss > 2000 ml ranges between 92 % and 96.2 %, with the PPV and NPV ranging between 71.4 % and 85.7 % and 61.3 % and 80.6 %, respectively. The JS group had a significant increase in blood loss (ml) (p = 0.003), transfusions (%) (p = 0.016), red blood cells (p = 0.002) and plasma (p = 0.002), admission to an postoperative intensive care unit (ICU) (%) (p = 0.002), hospitalization length (p < 0.001) and the need of cesarean hysterectomy (%) (p < 0.001). JS was independently correlated to cesarean hysterectomy (OR 25.6; 95 % CI 2.0:322.3, p = 0.012) and blood loss > 2000 ml (OR 16.6; 95 % CI 1.5:180.1, p = 0.021) also in a logistic regression model.
Conclusion JS is useful in predicting the increase in maternal morbidity: massive transfusion, admission to the ICU and cesarean hysterectomy related to intraoperative bleeding in patients with a previa AIP.
Macht über Leben und Tod
Authors: Lilienthal Georg 
Published: November 2017

Zusammenfassung
Psychiatrie im Nationalsozialismus Mit Beginn des Zweiten Weltkriegs eskalierte die Verfolgung psychisch kranker und geistig
behinderter Menschen zum Massenmord. Die an den Patientenmorden beteiligten Ärzte und Pflegekräfte verrichteten ihren Dienst routiniert und
im Bewusstsein der Macht über Leben und Tod. Ein Rückblick auf das vermutlich dunkelste Kapitel der deutschen Psychiatriegeschichte.
Phosphorylation of huntingtin at residue T3 is decreased in Huntington’s disease and modulates mutant huntingtin protein conformation
Authors: Cariulo Cristina , Azzollini Lucia , Verani Margherita , Martufi Paola , Boggio Roberto , Chiki Anass , Deguire Sean M. , Cherubini Marta , Gines Silvia , Marsh J. Lawrence , Conforti Paola , Cattaneo Elena , Santimone Iolanda , Squitieri Ferdinando , Lashuel Hilal A. , Petricca Lara , Caricasole Andrea 
Published: November 2017

Significance
The findings in this manuscript report on the identification of a posttranslational modification in the huntingtin protein (phosphorylation on residue T3 in the N17 region of the protein), which can revert the conformational effects of the Huntington’s disease (HD) mutation itself on the huntingtin protein and inhibit its aggregation properties in vitro. Using the first ultrasensitive immunoassay for a posttranslational modification of huntingtin protein, we demonstrate that pT3 levels are decreased in mutant huntingtin in preclinical models as well as in clinically relevant samples from HD patients. These findings are of high significance to Huntington’s disease biology, provide insights into mechanisms of Huntington’s disease pathogenesis, and open new opportunities for the development of therapeutics and diagnostics for Huntington’s disease.
Roles and Experiences of Parents in Necrotizing Enterocolitis
Authors: Gadepalli Samir K. , Canvasser Jennifer , Eskenazi Yael , Quinn Megan , Kim Jae H. , Gephart Sheila M. 
Published: November 2017

Background:
Although partnering with parents is important to improving neonatal outcomes, no studies have investigated what parents are taught, remember, or experience when their child is afflicted with necrotizing enterocolitis (NEC).

Purpose:
To characterize parental perceptions of communication and support they were given about NEC.

Methods:
An online survey was developed, reviewed for face validity, and then administered to parents whose child had experienced NEC. Quantitative data were analyzed using descriptive statistics, and qualitative data were analyzed using a qualitative descriptive approach.

Findings:
Parents (N = 110) wanted to know the risk factors and warning signs for NEC and wanted to be told as soon as their child was admitted to the neonatal intensive care unit (NICU). Information provided before diagnosis was felt to be poor by the majority of families, with only 32% feeling satisfied or very satisfied. No parent wrote that they were “scared” by information provided to them about NEC; in fact, parents were dissatisfied when they received “sugar-coated” information. Engaged parents were significantly more satisfied than those who were not informed, had their concerns and suggestions dismissed, or who had to advocate for their baby against clinician opposition (eg, activating the chain of command).

Implications for Practice:
Areas for quality improvement include better communication and collaboration with parents through early engagement in NEC prevention using modalities beyond verbal instruction.

Implications for Research:
More research is needed on how best to engage parents, especially to engage in prevention, and how doing so affects satisfaction and outcomes.
Associations of Race and Ethnicity With Patient-Reported Outcomes and Health Care Utilization Among Older Adults Initiating a New Episode of Care for Back Pain
Authors: Milani Carlo J. , Rundell Sean D. , Jarvik Jeffrey G. , Friedly Janna , Heagerty Patrick J. , Avins Andy , Nerenz David , Gold Laura S. , Turner Judith A. , Annaswamy Thiru , Nedeljkovic Srdjan S. , Suri Pradeep 
Published: November 2017

Study Design.
Secondary analysis of the Back Pain Outcomes using Longitudinal Data (BOLD) cohort study.

Objective.
To characterize associations of self-reported race/ethnicity with back pain (BP) patient-reported outcomes (PROs) and health care utilization among older adults with a new episode of care for BP.

Summary of Background Data.
No prior longitudinal studies have characterized associations between multiple race/ethnicity groups, and BP-related PROs and health care utilization in the United States.

Methods.
This study included 5117 participants ≥65 years from three US health care systems. The primary BP-related PROs were BP intensity and back-related functional limitations over 24 months. Health care utilization measures included common diagnostic tests and treatments related to BP (spine imaging, spine-related relative value units [RVUs], and total RVUs) over 24 months. Analyses were adjusted for multiple potential confounders including sociodemographics, clinical characteristics, and study site.

Results.
Baseline BP ratings were significantly higher for blacks vs. whites (5.8 vs. 5.0; P < 0.001). Participants in all race/ethnicity groups showed statistically significant, but modest improvements in BP over 24 months. Blacks and Hispanics did not have statistically significant improvement in BP-related functional limitations over time, unlike whites, Asians, and non-Hispanics; however, the magnitude of differences in improvement between groups was small. Blacks had less spine-related health care utilization over 24 months than whites (spine-related RVU ratio of means 0.66, 95% confidence interval [CI] 0.51–0.86). Hispanics had less spine-related health care utilization than non-Hispanics (spine-related RVU ratio of means 0.60; 95% CI 0.40–0.90).

Conclusion.
Blacks and Hispanics had slightly less improvement in BP-related functional limitations over time, and less spine-related health care utilization, as compared to whites and non-Hispanics, respectively. Residual confounding may explain some of the association between race/ethnicity and health outcomes. Further studies are needed to understand the factors underlying these differences and which differences reflect disparities.

Level of Evidence: 3
The Glucagon-Like Peptide-1 Analog Exenatide Increases Blood Glucose Clearance, Lactate Clearance, and Heart Rate in Comatose Patients After Out-of-Hospital Cardiac Arrest
Authors: Wiberg Sebastian , Kjaergaard Jesper , Schmidt Henrik , Thomsen Jakob Hartvig , Frydland Martin , Winther-Jensen Matilde , Lindholm Matias Greve , Høfsten Dan Eik , Engstrøm Thomas , Køber Lars , Møller Jacob Eifer , Hassager Christian 
Published: November 2017

Objectives:
To investigate the effects of the glucagon-like peptide-1 analog exenatide on blood glucose, lactate clearance, and hemodynamic variables in comatose, resuscitated out-of-hospital cardiac arrest patients.

Design:
Predefined post hoc analyzes from a double-blind, randomized clinical trial.

Setting:
The ICU of a tertiary heart center.

Patients:
Consecutive sample of adult, comatose patients undergoing targeted temperature management after out-of-hospital cardiac arrest from a presumed cardiac cause, irrespective of the initial cardiac rhythm.

Interventions:
Patients were randomized 1:1 to receive 6 hours and 15 minutes of infusion of either 17.4 μg of the glucagon-like peptide-1 analog exenatide (Byetta; Lilly) or placebo within 4 hours from sustained return of spontaneous circulation. The effects of exenatide were examined on the following prespecified covariates within the first 6 hours from study drug initiation: lactate level, blood glucose level, heart rate, mean arterial pressure, and combined dosage of norepinephrine and dopamine.

Measurements and Main Results:
The population consisted of 106 patients receiving either exenatide or placebo. During the first 6 hours from study drug initiation, the levels of blood glucose and lactate decreased 17% (95% CI, 8.9–25%; p = 0.0004) and 21% (95% CI, 6.0–33%; p = 0.02) faster in patients receiving exenatide versus placebo, respectively. Exenatide increased heart rate by approximately 10 beats per minute compared to placebo (p < 0.0001). There was no effect of exenatide on other hemodynamic variables.

Conclusions:
In comatose out-of-hospital cardiac arrest patients, infusion with exenatide lowered blood glucose and resulted in increased clearance of lactate as well as increased heart rate. The clinical importance of these physiologic effects remains to be investigated.