bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Arabidopsis HSP70 Sustains Cell Survival and Metastatic Potential of Breast Cancer Cells
Authors: Nigro Alessandra , Mauro Loredana , Giordano Francesca , Panza Salvatore , Iannacone Rina , Liuzzi Grazia Maria , Aquila Saveria , De Amicis Francesca , Cellini Francesco , Indiveri Cesare , Panno Maria Luisa 
Published: March 2016

Abstract
The chaperone HSP70 protein is widely present in many different tumors and its expression correlates with an increased cell survival, low differentiation, and poor therapeutic outcome in human breast cancer. The intracellular protein has prevalently a cytoprotective function, while the extracellular HSP70 mediates immunologic responses. Evolutionarily, HSPs are well conserved from prokaryotes to eukaryotes, and human HSP70 shows a strong similarity to that of plant origin. In the current article, we have tested the potential effect of recombinant HSP70, from Arabidopsis thaliana, on cell survival and metastatic properties of breast cancer cells. Our data show that HSP70 sustains cell viability in MCF-7 and MDA-MB-231 breast tumoral cells and increases Cyclin D1 and Survivin expression. The extracellular HSP70 triggers cell migration and the activation of MMPs particularly in MDA-MB-231 cells. Furthermore, under UV-induced stress condition, the low levels of phospho-AKT were increased by exogenous HSP70, together with the upregulation of Cyclin D1, particularly in the tumoral cell phenotype. On the other hand, UV increased TP53 expression, and the coincubation of HSP70 lowers the TP53 levels similar to the control. These findings correlate with the cytoprotective and antiapoptotic role of HSPs, as reported in different cellular contexts. This is the first study on mammary cells that highlights how the heterologous HSP70 from Arabidopsis thaliana sustains cell survival prevalently in breast cancer cell types, thus maintaining their metastatic potential. Therefore, targeting HSP70 would be of clinical importance since HSP70 blocking selectively targets tumor cells, in which it supports cell growth and survival. Mol Cancer Ther; 15(5); 1063–73. ©2016 AACR.
Favorable Cardiovascular Risk Profile Is Associated With Lower Healthcare Costs and Resource Utilization
Authors: Valero-Elizondo Javier , Salami Joseph A. , Ogunmoroti Oluseye , Osondu Chukwuemeka U. , Aneni Ehimen C. , Malik Rehan , Spatz Erica S. , Rana Jamal S. , Virani Salim S. , Blankstein Ron , Blaha Michael J. , Veledar Emir , Nasir Khurram 
Published: March 2016

Background—
The American Heart Association’s 2020 Strategic Goals emphasize the value of optimizing risk factor status to reduce the burden of morbidity and mortality. In this study, we aimed to quantify the overall and marginal impact of favorable cardiovascular risk factor (CRF) profile on healthcare expenditure and resource utilization in the United States among those with and without cardiovascular disease (CVD).

Methods and Results—

The study population was derived from the 2012 Medical Expenditure Panel Survey (MEPS). Direct and indirect costs were calculated for all-cause healthcare resource utilization. Variables of interest included CVD diagnoses (coronary artery disease, stroke, peripheral artery disease, dysrhythmias, or heart failure), ascertained by
International Classification of Diseases
, Ninth Edition, Clinical Modification codes, and CRF profile (hypertension, diabetes mellitus, hypercholesterolemia, smoking, physical activity, and obesity). Two-part econometric models were used to study expenditure data. The final study sample consisted of 15 651 MEPS participants (58.5±12 years, 54% female). Overall, 5921 (37.8%) had optimal, 7002 (44.7%) had average, and 2728 (17.4%) had poor CRF profile, translating to 54.2, 64.1, and 24.9 million adults in United States, respectively. Significantly lower health expenditures were noted with favorable CRF profile across CVD status. Among study participants with established CVD, overall healthcare expenditures with optimal and average CRF profile were $5946 and $3731 less compared with those with poor CRF profile. The respective differences were $4031 and $2560 in those without CVD.

Conclusions—
Favorable CRF profile is associated with significantly lower medical expenditure and healthcare utilization among individuals with and without established CVD.
Toward the Worker State, or Working for the State? Reorganization of Political Antagonisms in the Brazilian Amazon
Authors: Baletti Brenda 
Published: March 2016

The institutionalization of the Brazilian Workers’ Party has given rise to new tensions among emerging political actors, historic social movement mediator organizations, and the state. An analysis of the differences in strategies and practices between the Movement in Defense of Renascer and the Prainha Rural Worker’s Movement that emerged during the creation of the Renascer Extractive Reserve in the Lower Amazon highlights the fact that the movement’s emancipatory impulses indicate a break with the politics-as-usual of the union and the Workers’ Party more broadly. An examination of union political discourses and practices that seek to fold these emancipatory impulses back into the dominant logic indicates that the union continues to perform the work of the state—albeit a reconstituted one—both institutionally and effectively. A institucionalização do Partido dos Trabalhadores (PT) no Brasil tem criado novas tensões entre atores políticos emergentes, organizações mediadoras dos movimentos sociais históricos e o Estado. Uma análise das diferenças entre o Movimento em Defesa do Renascer e o Movimento dos Trabalhadores Rurais da Prainha, na Baixa Amazônia, enfatiza o fato de que os impulsos emancipatórios do movimento indicam um rompimento com os hábitos políticos de sindicatos e do Partido dos Trabalhadores de um modo geral. Um exame dos discursos políticos sindicais e das práticas que buscam a contenção desses impulsos emancipatórios, e tentam restaurá-los à lógica dominante, indica que o sindicato continua a desempenhar o trabalho do Estado—mesmo que reconstituído—tanto institucionalmente quanto efetivamente.
Estimation of overdiagnosis using short-term trends and lead time estimates uncontaminated by overdiagnosed cases: Results from the Norwegian Breast Screening Programme
Authors: Michalopoulos Dimitrios , Duffy Stephen W 
Published: March 2016

Background Estimating overdiagnosis in cancer screening is complicated. Using observational data, estimation of the expected incidence in the screening period and taking account of lead time are two major problems. Methods Using data from the Cancer Registry of Norway and the Norwegian Breast Cancer Screening Programme, we estimated incidence trends, using age-specific trends by year in the pre-screening period (1985–95). We also estimated sojourn time and sensitivity using interval cancers only. Thus, lead time estimates were uncontaminated by overdiagnosed cases. Finally, we derived estimates of overdiagnosis separately for all cancers, and for invasive cancers only, correcting for lead time, using two different methods. Results Our results indicate that overdiagnosis of all cancers, invasive and in situ, constituted 15–17% of all screen-detected cancers in 1996–2009. For invasive cancers only, the corresponding figures were -2 to 7% in the same period, suggesting that a substantial proportion of the overdiagnosis in the Norwegian Programme was due to ductal carcinoma in situ. Conclusion Using short-term trends, instead of long, prior to screening was more effective in predicting incidence in the screening epoch. In addition, sojourn time estimation using symptomatic cancers only avoids over-correction for lead time and consequently underestimation of overdiagnosis. Longer follow-up will provide more precise estimates of overdiagnosis.
Analysis of an electronic consultation program at an academic medical centre: Primary care provider questions, specialist responses, and primary care provider actions
Authors: Wrenn Katherine , Catschegn Sereina , Cruz Marisa , Gleason Nathaniel , Gonzales Ralph 
Published: March 2016

Introduction Electronic consultations (eConsults) increase access to specialty care, but little is known about the types of questions primary care providers (PCPs) ask through eConsults, and how they respond to specialist recommendations. Methods This is a retrospective descriptive analysis of the first 200 eConsults completed in the UCSF eConsult program. Participating PCPs were from eight adult primary care sites at the University of California, San Francisco (UCSF), USA. Medicine subspecialties participating were Cardiology, Endocrinology, Gastroenterology/hepatology, Hematology, Infectious diseases, Nephrology, Pulmonary medicine, Rheumatology, and Sleep medicine. We categorized eConsult questions into “diagnosis,” “treatment,” and/or “monitoring.” We performed medical record reviews to determine the percentage of specialist recommendations PCPs implemented, and the proportion of patients with a specialist visit in the same specialty as the eConsult, emergency department visit, or hospital admission during the subsequent six months. Results PCP questions related to diagnosis in 71% of cases, treatment in 46%, and monitoring in 21%. Specialist responses related to diagnosis in 76% of cases, treatment in 64%, and monitoring in 40%. PCPs ordered 79% of all recommended laboratory tests, 86% of recommended imaging tests and procedures, 65% of recommended new medications, and 73% of recommended medication changes. In the six months after the eConsult, 14% of patients had a specialist visit within the UCSF system in the same specialty as the eConsult. Discussion eConsults provide guidance to PCPs across the spectrum of patient care. PCPs implement specialists’ recommendations in the large majority of cases, and few patients subsequently require in-person specialty care related to the reason for the eConsult.
Determination of Factors Effected Dietary Glycemic Index in Turkish University Students
Authors: Gumus Huseyin , Akdevelioglu Yasemin , Bulduk Sıdıka 
Published: March 2016

<p style="text-align:justify">We aimed to determine any factors like as smoking, being regular activity affect dietary glycemic index in university students. This study was carried out at Gazi University, Ankara, Turkey. The participants were 577 randomly selected Turkish healthy female university students aged 17-32 years. The survey included a questionnaire that assessed demographic, 3-day self-reported nutrient intake. The dietary intake was based on the of the recipients. The 3 days included 1 weekend day and the previous or next 2 days. A weighted GI was designed to the diet of the participants with the use of values from the 2002 international table of GI values. BMI was calculated from measurements of height and weight. The differences between living area (house or dormitory)/regular physical activity (do or not)/smoking/nutrition education and mean dietary glycemic index was determined by Independent Sample t-Test. Dietary glycemic index was found high (>70) in 98.4% of participants. Mean dietary glycemic index was found 85.1±6.80 and it was significantly (p<0.05) differences between participants staying house or dormitory, doing physical activity or not, smoking or not and educated on nutrition or not. Participant’s who are smoking, staying dormitory and no educated about nutrition dietary glycemix index was found high than who are not smoking, staying house, doing regular physical activity and educated on nutrition. Conclusion and Implication: It’s important that educated students on health and nutrition to prevent their diets content high glycemic index. It can be help to prevent obesity in the future.</p>
Dayak Desa forest land use system as social capital to acquire forest management rights in West Kalimantan, Indonesia
Authors: ROSLINDA EMI 
Published: March 2016

Roslinda E. 2016. Dayak Desa forest land use system as social capital to acquire forest management rights in WestÂ Kalimantan, Indonesia. Biodiversitas 17: 177-184. People communities have an important role to play in biodiversity conservation. TheÂ ownership of land has often become a constraint on sustainable management of forest. The aim of this study was to explore theÂ management system of forest land use in Dayak Desa in West Kalimantan, Indonesia. Data collection was undertaken through fieldÂ observation, focus group discussion (FGD, and interviews with the community members. Dayak Desa has a several forest land useÂ systems including tawang semilas, tawang sebesai, tawang mersibung, tawang sepayan, tawang serimbak and bukit rentap protectionÂ forest, which each system has its own function and utilization. Regardless of the systemâ€™s differences, the forest land use systems haveÂ been supporting species diversity such as flora, fauna, and environmental services in Dayak Desa. This species diversity can supportÂ Dayak Desa community daily needs, regarding food, clothing, housing and other secondary needs. Forest utilization by Dayak DesaÂ community is suitably paired to what can be produced by the land, which is equipped with their own rules. The role and the rule inÂ managing, utilizing and protecting forest land use are Dayak Desa social capital. These are capital to acquire their rights to manage theÂ forest.
The effect of ultrasonic disintegration process conditions on the physicochemical characteristics of excess sludge
Authors: Tytła Malwina , Zielewicz Ewa 
Published: March 2016

Abstract
Ultrasonic disintegration, as a method of sludge pre-treatment (before the stabilization process), causes changes in their physicochemical characteristics. The aim of this study was to determine the influence of ultrasonic disintegration conditions (sonication) on the changes in the physicochemical characteristics of sonicated sludge, i.e. an increase in the content of organic substances in the supernatant, sludge dewaterability and flocs structure. Thickened and non-thickened excess sludge from the municipal wastewater treatment plant in Gliwice was disintegrated. The process was conducted, using a high-power disintegrator equipped with a lenticular horn. In order to determine the most favorable conditions, the sewage sludge was sonicated at a wave frequency of f=25 kHz (as a function of time), with a different samples volume (V1=0.5 and V2=1 L) and emitter position of 1 and the 2.5 cm from the bottom of the chamber in which the process was conducted. The disintegration of sewage sludge was carried out with a specific energy density (EV) in the range from 10 to 30 kWh/m3. The evaluation of the disintegration effects was based on changes in the physicochemical characteristics of the sludge and/or supernatant at the end of the process, expressed by commonly used and author’s disintegration indicators. The best results were obtained for the sludge disintegrated with a volume of V2=1 L and the emitter position of 2.5 cm from the bottom of the chamber. The study confirms that in various operating conditions of ultrasonic disintegration, there is a possibility for obtaining different effects which may influence the course of anaerobic stabilization and quality of the final products of the process.
The effect of temperature on the efficiency of industrial wastewater nitrification and its (geno)toxicity
Authors: Gnida Anna , Wiszniowski Jarosław , Felis Ewa , Sikora Jan , Surmacz-Górska Joanna , Miksch Korneliusz 
Published: March 2016

AbstractThe paper deals with the problem of the determination of the effects of temperature on the efficiency of the nitrification process of industrial wastewater, as well as its toxicity to the test organisms. The study on nitrification efficiency was performed using wastewater from one of Polish chemical factories. The chemical factory produces nitrogen fertilizers and various chemicals. The investigated wastewater was taken from the influent to the industrial mechanical-biological wastewater treatment plant (WWTP). The WWTP guaranteed high removal efficiency of organic compounds defined as chemical oxygen demand (COD) but periodical failure of nitrification performance was noted in last years of the WWTP operation. The research aim was to establish the cause of recurring failures of nitrification process in the above mentioned WWTP. The tested wastewater was not acutely toxic to activated sludge microorganisms. However, the wastewater was genotoxic to activated sludge microorganisms and the genotoxicity was greater in winter than in spring time. Analysis of almost 3 years’ period of the WWTP operation data and laboratory batch tests showed that activated sludge from the WWTP under study is very sensitive to temperature changes and the nitrification efficiency collapses rapidly under 16°C. Additionally, it was calculated that in order to provide the stable nitrification, in winter period the sludge age (SRT) in the WWTP should be higher than 35 days.
Operation mode and external carbon dose as determining factors in elemental composition and morphology of aerobic granules
Authors: Cydzik-Kwiatkowska Agnieszka , Rusanowska Paulina , Głowacka Katarzyna 
Published: March 2016

Abstract
The elemental composition and morphology of aerobic granules in sequencing batch reactors (GSBRs) treating high-nitrogen digester supernatant was investigated. The investigation particularly focused on the effect of the number of anoxic phases (one vs. two) in the cycle and the dose of external organics loading (450 mg COD/(L·cycle) vs. 540 mg COD/(L·cycle)) on granule characteristics.
Granules in all reactors were formed of many single cells of rod and spherical bacteria. Addition of the second anoxic phase in the GSBR cycle resulted in enhanced settling properties of the granules of about 10.6% and at the same time decreased granule diameter of about 19.4%. The study showed that external organics loading was the deciding factor in the elemental composition of biomass. At 540 mg COD/(L·cycle) the granules contained more weight% of C, S and N, suggesting more volatile material in the granule structure. At lower organics loadings granules had the higher diameter of granules which limited the diffusion of oxygen and favored precipitation of mineral compounds in the granule interior. In this biomass higher content of Mg, P and Ca, was observed.
Inorganic nanomaterials in the aquatic environment: behavior, toxicity, and interaction with environmental elements
Authors: Krzyżewska Iwona , Kyzioł-Komosińska Joanna , Rosik-Dulewska Czesława , Czupioł Justyna , Antoszczyszyn-Szpicka Patrycja 
Published: March 2016

AbstractThe aim of this paper is to present characteristics, toxicity and environmental behavior of nanoparticles (NPs) (silver, copper, gold, zinc oxide, titanium dioxide, iron oxide) that most frequently occur in consumer products. In addition, NPs are addressed as the new aquatic environmental pollutant of the 21stcentury. NPs are adsorbed onto particles in the aquatic systems (clay minerals, fulvic and humic acids), or they can adsorb environmental pollutants (heavy metal ions, organic compounds). Nanosilver (nAg) is released from consumer products into the aquatic environment. It can threaten aquatic organisms with high toxicity. Interestingly, copper nanoparticles (Cu-NPs) demonstrate higher toxicity to bacteria and aquatic microorganisms than those of nanosilver nAg. Their small size and reactivity can cause penetration into the tissues and interfere with the metabolic systems of living organisms and bacterial biogeochemical cycles. The behavior of NPs is not fully recognized. Nevertheless, it is known that NPs can agglomerate, bind with ions (chlorides, sulphates, phosphates) or organic compounds. They can also be bound or immobilized by slurry. The NPs behavior depends on process conditions, i.e. pH, ionic strength, temperature and presence of other chemical compounds. It is unknown how NPs behave in the aquatic environment. Therefore, the research on this problem should be carried out under different process conditions. As for the toxicity, it is important to understand where the differences in the research results come from. As NPs have an impact on not only aquatic organisms but also human health and life, it is necessary to recognize their toxic doses and know standards/regulations that determine the permissible concentrations of NPs in the environment.
Shape Optimisation of Multi-Chamber Acoustical Plenums Using BEM, Neural Networks, and GA Method
Authors: Chang Ying-Chun , Cheng Ho-Chih , Chiu Min-Chie , Chien Yuan-Hung 
Published: March 2016

Abstract
Research on plenums partitioned with multiple baffles in the industrial field has been exhaustive. Most researchers have explored noise reduction effects based on the transfer matrix method and the boundary element method. However, maximum noise reduction of a plenum within a constrained space, which frequently occurs in engineering problems, has been neglected. Therefore, the optimum design of multi-chamber plenums becomes essential. In this paper, two kinds of multi-chamber plenums (Case I: a two-chamber plenum that is partitioned with a centre-opening baffle; Case II: a three-chamber plenum that is partitioned with two centre-opening baffles) within a fixed space are assessed.
In order to speed up the assessment of optimal plenums hybridized with multiple partitioned baffles, a simplified objective function (OBJ) is established by linking the boundary element model (BEM, developed using SYSNOISE) with a polynomial neural network fit with a series of real data – input design data (baffle dimensions) and output data approximated by BEM data in advance. To assess optimal plenums, a genetic algorithm (GA) is applied. The results reveal that the maximum value of the transmission loss (TL) can be improved at the desired frequencies. Consequently, the algorithm proposed in this study can provide an efficient way to develop optimal multi-chamber plenums for industry.
Sound Radiation of a Pulsating Sphere in the Outlet of a Hard/Soft Semi-Spherical Cavity in a Flat Screen
Authors: Rdzanek Wojciech P. 
Published: March 2016

Abstract
A rigorous analysis of sound radiation by a pulsating sphere forming a resonator together with a semi-spherical cavity is presented. Both hard and soft boundaries are considered, as well as mixed. The problem is solved by dividing the entire region into two subregions, one surrounding the sphere and containing the cavity and the other for the remaining half-space. The continuity conditions are applied to obtain the acoustic pressure. Then the acoustic radiation resistance is calculated both in the near- and far-field. The acoustic radiation reactance is calculated in the impedance approach. The resonance frequencies are determined, for which a significant growth of the sound pressure level is observed as well as the sound field directivity. The accuracy and convergence of these rigorous results has been examined empirically.
The Uncertainty of Noise Composed of Separate Sound Events
Authors: Makarewicz Rufin , Gołębiewski Roman 
Published: March 2016

Abstract
Transportation noise is a main source of noise pollution. It is assumed that it consists of recognizable noise events which come from moving aircrafts, trains and boats. The noise of an isolated sound event is assessed by the sound exposure level, LAE. Much legislation and many regulations and guidelines employ the A-weighted time-average sound level, LAeq,T, with the time interval T of one hour or longer. LAE measurements enable an approximation of LAeq,T. The key point is the uncertainty of this approximation. It has been shown that an increase in the number of LAE categories brings about a decrease in uncertainty. For illustrative purposes, LAE measurements of aircrafts taking off and landing were carried out.