bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendampingan Pengaplikasian Hasil Desain Pada Kelompok Remaja Jimpitan Kelurahan Batu Jaya, Kota Tangerang
Authors: Pangestu Ilham Aji , Suseno Ari , Fadhlillah Muhammad Rizqi , Kurniawan Irvan Arif 
Published: September 2024

Kegiatan Pengabdian Kepada Masyarakat ini merupakan kegiatan tahap 2 (dua) berdasarkan roadmap kegiatan Pengabdian Kepada Masyarakat pada kelompok remaja Jimpitan. Kelompok remaja Jimpitan dalam hal ini terhimpun dalam Pusat Informasi, Konseling, Komunikasi Remaja (PIKKR). Tujuan dari kegiatan Pengabdian Kepada Masyarakat tahap 2 (dua) ini adalah kelompok remaja PIKKR dapat mengaplikasikan hasil desain yang sudah disusun kedalam suatu media tertentu. Metode yang digunakan pada Pengabdian Kepada Masyarakat tahap 2 (dua) ini menggunkan metode pelatihan. Dengan metode pelatihan, peserta dalam hal ini kelompok remaja PIKKR diberikan materi mengenai dasar-dasar desain, aplikasi yang digunakan serta pemahaman dalam mengaplikasikan kedalam suatu media tertentu. Materi-materi tersebut selain diberikan secara teori, juga dilaksanakan melalui praktik. Berdasarkan kegiatan Pengabdian Kepada Masyarakat tahap 2 (dua) yang sudah dilaksanakan, tujuan utama dari kegiatan ini belum tercapai. Hal tersebut dipengaruhi oleh beberapa faktor kendala. Faktor-faktor kendala tersebut diantaranya, pertama yaitu kendala dalam hal instal aplikasi adobe illustrator. Kedua, tidak seluruhnya anggota kelompok remaja PIKKR memiliki laptop. Ketiga, kurangnya intensitas pendampingan kepada kelompok remaja PIKKR. Untuk kedepannya, saran yang diberikan diantaranya pertama, memperkuat intensitas pendampingan dan monitoring, baik dilakukan secara formal ataupun non formal. Kedua, memperkuat komunikasi dengan kelompok remaja PIKKR, dan ketiga, mengalokasikan laptop yang khusus digunakan oleh kelompok remaja PIKKR.
Peningkatan Kualitas UMKM Dalam Pemasaran Teh Telang Berbasis Digital Di Desa Wage, Kecamatan Taman
Authors: Kurniawan Bagus Ananda , Prasetyo Farrel Eko , Halawa Tita Vestalenti 
Published: September 2024

Warga RW 13 Desa Wage di Kecamatan Taman Kabupaten Sidoarjo, Jawa Timur, membudidayakan bunga telang di halaman rumah. Masyarakat menganggap bunga telang adalah tanaman rambat biasa. Oleh karena itu, untuk mengetahui keuntungan bunga telang, pemberdayaan masyarakat harus dilakukan. Kehidupan masyarakat RW 13 Desa Wage Kecamatan Taman memiliki beragam UMKM hasil olahan bunga telang. Usaha olahan produk ini adalah mata pencaharian utama dan memiliki kebun kecil bunga telang di wilayah asrama polisi RW 13 Desa Wage dan sekitar wilayah Desa Wage RW 13 ini salah satu tempat sebagai pengembangan usaha teh telang dan memiliki UMKM hasil olahan bunga telang berupa teh telang dan serbuk teh telang. RW 13 Desa wage ini sebagai tempat kegiatan pengabdian kepada masyarakat mandiri yang dilaksanakan oleh Tim Dosen bidang pengabdian masyarakat dan kelompok 1 KKN gelombang 2. Adapun yang menjadi mitra kegiatan pengabdian kepada masyarakat mandiri ini adalah Kelompok Kuliah Kerja Nyata (KKN), para warga beserta pengurus Rukun Warga (RW) 3, dan UMKM Asmantoga RW 13 Teh telang Desa Wage Kecamatan Taman. Kegiatan pengabdian kepada masyarakat mandiri ini dilaksanakan dengan metode pelatihan dan pendampingan. Pelatihan dilaksanakan dalam sebuah workshop yang memberikan pendalaman materi tentang pentingnya penghijauan dan menumbuhkan kepedulian penghijauan, mendesain label produk, digital marketing e-commerce dalam memasarkan komoditi teh telang, menambahkan konten berita artikel di website resmi Desa Wage, mengadakan lomba mengaji untuk meningkatkan literasi anak usia dini.
Pharmacokinetics of a new drug based on polysaccharide from acorus calamus in an experiment on laboratory animals
Authors: Krivoshchekov S. V. , Yanovskaya E. A. , Gurto R. V. , Bryushinina O. S. , Isakov D. A. , Zykova A. V. , Zyuz’kova Yu. G. , Guryev A. M. , Udut V. V. , Belousov M. V. 
Published: September 2024

Introduction. α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane is a water-soluble heteropolysaccharide isolated from the rhizomes of calamus marsh (Acorus calamus L.) and has antitumor, antimetastatic, immunomodulatory and hepatoprotective effects, protective effect on hematopoiesis suppressed during cytostatic treatment.Aim. Validation of the bioanalytical method for the determination of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane Acorus calamus L. and the study of the pharmacokinetic parameters of a new drug based on it after a single administration of 120 mg/kg to rats and 24, 30, 42 mg/kg to rabbits.Materials and methods. In the study, 8 male rats (average weight – 300 g) were used as the main animals. 6 male rabbits (average body weight – 2.5 kg) were used as an additional animal species. The dose assessment experiment was carried out on 24 rabbits (body weight – 2.3–2.5 kg). The quantitative determination of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane Acorus calamus L. in the blood plasma of animals was carried out by HPLC-MS/MS, the analytical form was galacturonic acid. The method of sample preparation of biological samples included the stage of enzymatic hydrolysis of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane Acorus calamus L. with a solution of pectinase for the formation of galacturonic acid.Results and discussions. The pharmacokinetics of the new drug α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane Acorus calamus L. was studied after a single dose of 120 mg/kg to rats and 24, 30, 42 mg/kg to rabbits. The average residence time of the drug in the rabbit's body was 1.0 ± 0.21 hours, the half-life was 0.7 ± 0.15 hours. The average residence time of the drug in the rat body was 1.16 hours, the half-life T1/2 was 0.80 hours. After a single intravenous administration to rabbits of 24, 30 and 42 mg/kg, the maximum concentration in the systemic bloodstream is reached 5 minutes after administration and averages 133.95 ± 27.04, 145.86 ± 40.90 and 226.13 ± 41.27 µg/ml, respectively, the half-life ranged from 0.22 to 0.26 h, the half-life from 3.52 to 3.96 hours.Conclusion. The pharmacokinetic parameters of α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane Acorus calamus L. were evaluated in animals based on the use of the method of quantitative determination of galacturonic acid by HPLC-MS/MS.
Causal role of immune cells in diabetic nephropathy: a bidirectional Mendelian randomization study
Authors: Wang Shang-Yuan , Yu Yang , Ge Xiao-Li , Pan Shuming 
Published: September 2024

BackgroundDiabetic nephropathy (DN) stands as a pervasive chronic renal disease worldwide, emerging as the leading cause of renal failure in end-stage renal disease. Our objective is to pinpoint potential immune biomarkers and evaluate the causal effects of prospective therapeutic targets in the context of DN.MethodsWe employed Mendelian randomization (MR) analysis to examine the causal associations between 731 immune cell signatures and the risk of DN. Various analytical methods, including inverse-variance weighted (IVW), MR-Egger, weighted median, simple mode, and weighted mode, were employed for the analysis. The primary analytical approach utilized was the inverse-variance weighted (IVW) method. To ensure the reliability of our findings, we conducted comprehensive sensitivity analyses to assess the robustness, heterogeneity, and presence of horizontal pleiotropy in the results. Statistical powers were also calculated. Ultimately, a reverse Mendelian randomization (MR) analysis was conducted to assess the potential for reverse causation.ResultsAfter Benjamini &amp; Hochberg (BH) correction, four immunophenotypes were identified to be significantly associated with DN risk: HLA DR on Dendritic Cell (OR=1.4460, 95% CI = 1.2904~1.6205, P=2.18×10−10, P.adjusted= 1.6×10−7), HLA DR on CD14+ CD16− monocyte (OR=1.2396, 95% CI=1.1315~1.3580, P=3.93×10−6, P.adjusted = 0.00143). HLA DR on CD14+ monocyte (OR=1.2411, 95% CI=1.12957~1.3637, P=6.97×10−6, P.adjusted=0.0016), HLA DR on plasmacytoid Dendritic Cell (OR=1.2733, 95% CI= 1.1273~1.4382, P= 0.0001, P.adjusted = 0.0183). Significant heterogeneity of instrumental variables was found in the four exposures, and significant horizontal pleiotropy was only found in HLA DR on Dendritic Cell. The bidirectional effects between the immune cells and DN were not supported.ConclusionOur research illustrated the intimate association between immune cells and DN, which may contribute to a deeper understanding of the intricate mechanisms underlying DN and aid in the identification of novel intervention target pathways.
Environmental regulation, industrial transformation, and green economy development
Authors: Shao Huimin , Peng Qiong , Zhou Fei , Wider Walton 
Published: September 2024

BackgroundGreen development, which is crucial because of the escalating ecological and environmental issues, is largely driven by industrial transformation and environmental legislation.PurposeThe paper aims to examine the combined effects of environmental regulations and industrial change on the green economy, based on their separate effects.MethodsWe use a fixed-effects regression on panel data covering 30 provinces in China in 2010–2020.ResultsWe reach the following conclusions: environmental regulations and rationalization of the industrial structure both support growth in the green economy, but advancement in the industrial structure hinders it. The combined effect of environmental regulations and advancement and rationalization in the industrial structure has a significantly positive impact on growth in the green economy; it is most pronounced in regions with higher resource endowments and economic development.DiscussionBy providing specific empirical facts, this study clarifies the effects of industrial transformation and environmental regulations on growth in the green economy, contributing to enriching the literature and helping to pave the way to sustainable development.
Response of radial growth of Dahurian larch (Larix gmelinii) to climate factors at different altitudes in the northern part of the Greater Khingan Mountains
Authors: Luo Taoran , Wang Zhaopeng , Zhang Dongyou , Li Xiangyou , Wang Xinrui 
Published: September 2024

IntroductionTo investigate the differences in the response of radial growth of larch (Larix gmelinii) at varying altitude gradients to climatic factors, this study established tree-ring width chronologies of Larix gmelinii at altitudes of 900 m, 1,120 m and 1,300 m based dendroclimatology.MethodsWe also employed R packages including “dplR,” “static” and “moving” to determine the correlation between larch at different altitudes and climatic factors and to explore the sensitivity and stability between radial growth and the climate of larch at different altitudes in the northern part of the Greater Khingan Mountains Range in Northeast China.ResultsThe results revealed the radial growth of larch in high altitude areas to exhibit a downward trend due to climate warming, while the growth of trees in intermediate and low altitude areas showed an upward trend. The growth–climate relationship exhibited a significant negative correlation between radial growth in low altitude larch (900 m) and temperatures in the current winter (February and March) and growing season (July, October of the current year, and October of the previous year) (p &lt; 0.05). Moreover, high altitude larch (1,300 m) showed a significant negative correlation with temperature in January, March, and October of the current year (p &lt; 0.05). However, in intermediate altitude areas (1,120 m), the radial growth of trees was significantly positively correlated with the temperature of the growing season (May, June and August). Precipitation in April was observed to promote the radial growth of low-altitude larch. Moving correlation analysis revealed that the inhibitory effect of low temperatures in winter and high temperatures in the growing season on the radial growth of larch at three altitudes gradually strengthened, transitioning from significant negative/positive correlations to significant positive/negative correlations along the altitudinal gradient.DiscussionLarch trees in high and low altitudes exhibited an increased sensitivity to winter and spring precipitation, while in the intermediate region, the inhibitory effect of growing season precipitation on larch radial growth continued to intensify. The results of the study have an important reference value for the in-depth understanding of the growth dynamics of Larix gmelinii natural forests in the northern part of the Greater Khingan Mountains under climate change.
Radiation immunodynamics in patients with glioblastoma receiving chemoradiation
Authors: Sloan Lindsey , Sen Rupashree , Liu Chunnan , Doucet Michele , Blosser Lee , Katulis Lisa , Kamson David O. , Grossman Stuart , Holdhoff Matthias , Redmond Kristin J. , Quon Harry , Lim Michael , Eberhart Charles , Pardoll Drew M. , Hu Chen , Ganguly Sudipto , Kleinberg Lawrence R. 
Published: September 2024

IntroductionThis is a prospective, rigorous inquiry into the systemic immune effects of standard adjuvant chemoradiotherapy, for WHO grade 4, glioblastoma. The purpose is to identify peripheral immunologic effects never yet reported in key immune populations, including myeloid-derived suppressor cells, which are critical to the immune suppressive environment of glioblastoma. We hypothesize that harmful immune-supportive white blood cells, myeloid derived suppressor cells, expand in response to conventionally fractionated radiotherapy with concurrent temozolomide, essentially promoting systemic immunity similar what is seen in chronic diseases like diabetes and heart disease.Methods16 patients were enrolled in a single-institution, observational, immune surveillance study where peripheral blood was collected and interrogated by flow cytometry and RNAseq. Tumor tissue from baseline assessment was analyzed with spatial proteomics to link peripheral blood findings to baseline tissue characteristics.ResultsWe identified an increase in myeloid-derived suppressor cells during the final week of a six-week treatment of chemoradiotherapy in peripheral blood of patients that were not alive at two years after diagnosis compared to those who were living. This was also associated with a decrease in CD8+ T lymphocytes that produced IFNγ, the potent anti-tumor cytokine.DiscussionThese data suggest that, as in chronic inflammatory disease, systemic immunity is impaired following delivery of adjuvant chemoradiotherapy. Finally, baseline investigation of myeloid cells within tumor tissue did not differ between survival groups, indicating immune surveillance of peripheral blood during adjuvant therapy may be a critical missing link to educate our understanding of the immune effects of standard of care therapy for glioblastoma.
Neutrophil-fibroblast crosstalk drives immunofibrosis in Crohn’s disease through IFNα pathway
Authors: Gavriilidis Efstratios , Divolis Georgios , Natsi Anastasia-Maria , Kafalis Nikolaos , Kogias Dionysios , Antoniadou Christina , Synolaki Evgenia , Pavlos Evgenios , Koutsi Marianna A. , Didaskalou Stylianos , Papadimitriou Evangelos , Tsironidou Victoria , Gavriil Ariana , Papadopoulos Vasileios , Agelopoulos Marios , Tsilingiris Dimitrios , Koffa Maria , Giatromanolaki Alexandra , Kouklakis Georgios , Ritis Konstantinos , Skendros Panagiotis 
Published: September 2024

IntroductionCrohn’s disease (CD) is characterized by chronic inflammation and intestinal fibrosis leading to lifelong complications. However, the disease pathogenesis remains elusive, and the therapeutic options are limited. Here, we investigated the interaction between neutrophils and intestinal fibroblasts in the development of CD immunofibrosis, a disease mechanism predisposing to inflammatory and fibrotic complications.MethodsPeripheral neutrophils, enriched neutrophil extracellular traps (eNETs), serum, primary intestinal fibroblasts (PIFs) and intestinal biopsies from CD, ulcerative colitis (UC) patients, and healthy individuals (HI), were studied. Transcriptome analysis of neutrophils, multi-cytokine profiling and cell-based functional assays at mRNA/protein level were performed.ResultsCompared to UC, PIFs from CD patients, independently to the presence of strictures, displayed a distinct pro-fibrotic phenotype characterized by negative Krüppellike Factor-2 (KLF2) and increased cellular communication network factor-2 (CCN2) expression leading to collagen production. In both UC and CD, PIFs-derived IL-8 acted as a culprit chemoattractant for neutrophils in the intestine, where CD neutrophils were accumulated close to fibrotic lesions. Functionally, only CD neutrophils via eNETs induced a CD-like phenotype in HI PIFs, suggesting their fibrotic plasticity. High IFNa in serum and IFΝ-responsive signature in peripheral neutrophils were observed in CD, distinguishing it from UC. Moreover, CD serum stimulated the release of fibrogenic eNETs from neutrophils in an IFNa-dependent manner, suggesting the priming role of IFNa in circulating neutrophils. Inhibition of eNETs or JAK signaling in neutrophils or PIFs prevented the neutrophil-mediated fibrotic effect on PIFs. Furthermore, both serum IFNa levels and mRNA levels of key IFN signaling components in neutrophils were wellcorrelated with CD severity.ConclusionsThis study reveals the important role of the IFNa/neutrophil/fibroblast axis in CD immunofibrosis, suggesting candidate biomarkers and putative therapeutic targets.
Unraveling the role of ADAMs in clinical heterogeneity and the immune microenvironment of hepatocellular carcinoma: insights from single-cell, spatial transcriptomics, and bulk RNA sequencing
Authors: Chen Junhong , Yuan Qihang , Guan Hewen , Cui Yuying , Fu Chang , Wei Tianfu , Liu Kai 
Published: September 2024

BackgroundHepatocellular carcinoma (HCC) is a prevalent and heterogeneous tumor with limited treatment options and unfavorable prognosis. The crucial role of a disintegrin and metalloprotease (ADAM) gene family in the tumor microenvironment of HCC remains unclear.MethodsThis study employed a novel multi-omics integration strategy to investigate the potential roles of ADAM family signals in HCC. A series of single-cell and spatial omics algorithms were utilized to uncover the molecular characteristics of ADAM family genes within HCC. The GSVA package was utilized to compute the scores for ADAM family signals, subsequently stratified into three categories: high, medium, and low ADAM signal levels through unsupervised clustering. Furthermore, we developed and rigorously validated an innovative and robust clinical prognosis assessment model by employing 99 mainstream machine learning algorithms in conjunction with co-expression feature spectra of ADAM family genes. To validate our findings, we conducted PCR and IHC experiments to confirm differential expression patterns within the ADAM family genes.ResultsGene signals from the ADAM family were notably abundant in endothelial cells, liver cells, and monocyte macrophages. Single-cell sequencing and spatial transcriptomics analyses have both revealed the molecular heterogeneity of the ADAM gene family, further emphasizing its significant impact on the development and progression of HCC. In HCC tissues, the expression levels of ADAM9, ADAM10, ADAM15, and ADAM17 were markedly elevated. Elevated ADAM family signal scores were linked to adverse clinical outcomes and disruptions in the immune microenvironment and metabolic reprogramming. An ADAM prognosis signal, developed through the utilization of 99 machine learning algorithms, could accurately forecast the survival duration of HCC, achieving an AUC value of approximately 0.9.ConclusionsThis study represented the inaugural report on the deleterious impact and prognostic significance of ADAM family signals within the tumor microenvironment of HCC.
The value of lymphocyte-to-C-reactive protein ratio for predicting clinical outcomes in patients with sepsis in intensive care unit: a retrospective single-center study
Authors: Song Chao , Hu Zhenkui , Zhang Jinhui 
Published: September 2024

BackgroundThe lymphocyte-to-C-reactive protein ratio (LCR) was a novel biomarker of inflammation that had been implicated in various diseases. Nevertheless, the role of LCR in the context of sepsis patients admitted to the Intensive Care Unit (ICU) had not been thoroughly elucidated. This study aimed to determine the significance of the LCR in predicting the prognosis of sepsis patients within ICU.MethodsA sample of sepsis patients requiring ICU care was selected from the Affiliated Hospital of Jiangsu University. These patients were then segmented into four quartiles based on their LCR levels. The primary endpoint of the study was 30-day mortality and the secondary endpoint was the occurrence of Acute Kidney Injury (AKI). Survival analysis, via the Kaplan-Meier method and log-rank test, was conducted to assess survival rates. Cox proportional hazards regression and logistic regression models were employed to investigate the association between LCR and clinical outcomes. Additional subgroup analyses were conducted to evaluate the influence of other confounding factors on the relationship between LCR and patient outcomes.ResultsA total of 1,123 patients were enrolled in this study, with a median age of 75 (65–84) years, and 707 (63.0%) of them were male. The 30-day mortality rate was 28.1%, while the incidence of AKI was 45.6%. A progressive decrease in LCR levels was found to be associated with an increased cumulative incidence of 30-day mortality (log-rank P &lt; 0.001). Multivariable Cox proportional hazards analyses demonstrated that LCR was an independent predictor of 30-day mortality [per 1-unit increase in LCR: HR (95%CI): 0.370 (0.142–0.963); P = 0.042]. Additionally, multivariable logistic regression analysis revealed a significant association between LCR and AKI occurrence [per 1-unit increase in LCR: OR (95%CI): 0.541 (0.307–0.953); P = 0.034]. Furthermore, subgroup analysis indicated a stronger correlation for patients aged over 65 years compared to those aged 65 or younger (p for interaction &lt;0.05) in predicting 30-day mortality or AKI occurrence based on LCR.ConclusionA reduction in LCR was notably linked to 30-day mortality and the occurrence of AKI in sepsis patients. These findings suggested that LCR could potentially serve as a valuable tool in identifying sepsis patients at a heightened risk of adverse outcomes.