bluuebunny/crossref_metadata_2025_split huggingface dataset:
Borreliaceae
Authors: Barbour Alan G. 
Published: December 2017

Bor.rel.i.a.ce'ae. N.L. fem. n.Borreliatype genus of the family; suff. ‐aceaeending to denote a family; N.L. fem. pl. n.Borreliaceae, the family ofBorrelia.Spirochaetes / Spirochaetia / Spirochaetales / BorreliaceaeCells are helical with regular or irregular coils, 0.2–0.3 µm in diameter and 10–40 µm in length. Cells do not have hooked ends. Motile. Inner and outer membrane with periplasmic flagella with 7–20 subterminal insertion points. Aniline‐stain‐positive. Microaerophilic. Most members of the family cultivable in complex media that includesN‐acetylglucosamine. Optimum growth between 33°C and 38°C. Diamino acid of peptidoglycan is ornithine. Lacks a lipopolysaccharide. Linear chromosome and plasmids with hairpin telomeres. The family currently accommodates the generaBorreliaandBorreliella. Members of the family are host‐associated organisms that are transmitted between vertebrate reservoirs by a hematophagous arthropod, in all but one case, a tick. Members include the agents of relapsing fever, Lyme disease, and avian spirochetosis.DNA G + C content (mol%): 26–30.Type genus:BorreliaSwellengrebel 1907, 562AL.
Light‐Responsive Biodegradable Nanorattles for Cancer Theranostics
Authors: Li Chunxiao , Zhang Yifan , Li Zhiming , Mei Enci , Lin Jing , Li Fan , Chen Cunguo , Qing Xialing , Hou Liyue , Xiong Lingling , Hao Hui , Yang Yun , Huang Peng 
Published: December 2017

AbstractCancer nanotheranostics, integrating both diagnostic and therapeutic functions into nanoscale agents, are advanced solutions for cancer management. Herein, a light‐responsive biodegradable nanorattle‐based perfluoropentane‐(PFP)‐filled mesoporous‐silica‐film‐coated gold nanorod (GNR@SiO2‐PFP) is strategically designed and prepared for enhanced ultrasound (US)/photoacoustic (PA) dual‐modality imaging guided photothermal therapy of melanoma. The as‐prepared nanorattles are composed of a thin mesoporous silica film as the shell, which endows the nanoplatform with flexible morphology and excellent biodegradability, as well as large cavity for PFP filling. Upon 808 nm laser irradiation, the loaded PFP will undergo a liquid–gas phase transition due to the heat generation from GNRs, thus generating nanobubbles followed by the coalescence into microbubbles. The conversion of nanobubbles to microbubbles can improve the intratumoral permeation and retention in nonmicrovascular tissue, as well as enhance the tumor‐targeted US imaging signals. This nanotheranostic platform exhibits excellent biocompatibility and biodegradability, distinct gas bubbling phenomenon, good US/PA imaging contrast, and remarkable photothermal efficiency. The results demonstrate that the GNR@SiO2‐PFP nanorattles hold great potential for cancer nanotheranostics.
A distinct immunophenotype identifies a subset of NPM1‐mutated AML with TET2 or IDH1/2 mutations and improved outcome
Authors: Mason Emily F. , Kuo Frank C. , Hasserjian Robert P. , Seegmiller Adam C. , Pozdnyakova Olga 
Published: December 2017

AbstractRecent work has identified distinct molecular subgroups of acute myeloid leukemia (AML) with implications for disease classification and prognosis. NPM1 is one of the most common recurrently mutated genes in AML. NPM1 mutations often co‐occur with FLT3‐ITDs and mutations in genes regulating DNA methylation, such as DNMT3A, TET2, and IDH1/2. It remains unclear whether these genetic alterations are associated with distinct immunophenotypic findings or affect prognosis. We identified 133 cases of NPM1‐mutated AML and correlated sequencing data with immunophenotypic and clinical findings. Of 84 cases (63%) that lacked monocytic differentiation (“myeloid AML”), 40 (48%) demonstrated an acute promyelocytic leukemia‐like (APL‐like) immunophenotype by flow cytometry, with absence of CD34 and HLA‐DR and strong myeloperoxidase expression, in the absence of a PML‐RARA translocation. Pathologic variants in TET2, IDH1, or IDH2 were identified in 39/40 APL‐like cases. This subset of NPM1‐mutated AML was associated with longer relapse‐free and overall survival, when compared with cases that were positive for CD34 and/or HLA‐DR. The combination of NPM1 and TET2 or IDH1/2 mutations along with an APL‐like immunophenotype identifies a distinct subtype of AML. Further studies addressing its biology and clinical significance may be especially relevant in the era of IDH inhibitors and recent work showing efficacy of ATRA therapy in NPM1 and IDH1‐mutated AML.
Nitrogen doped nano porous graphene as a sorbent for separation and preconcentration trace amounts of Pb, Cd and Cr by Ultrasonic assisted in‐syringe dispersive micro solid phase extraction
Authors: Lotfi Zahra , Zavvar Mousavi Hassan , Sajjadi S. Maryam 
Published: December 2017

Nitrogen doped nano porous graphene was used as an efficient sorbent in solid‐phase extraction process for simultaneous separation and pre‐concentration of metal ions lead (II), cadmium(II), and chromium(III)) in biological samples. Ultrasonic assisted in‐syringe dispersive micro solid phase extraction coupled with micro sampling atomic absorption spectrometry was utilized for the determination of metal ions. Nitrogen doped nano porous graphene was synthesized as a nano sorbent by chemical vapour deposition method. Methane and aniline were used as carbon and nitrogen sources. The characterization of sorbent was performed by field emission scanning electron microscope, transmission electron microscopy, atomic force microscope, fourier transform infrared, chemical element analysis and raman analysis. Effective parameters on the extraction efficiency such as pH, sorbent dosage, eluent volume and eluent concentration were optimized by central composite design and desirability function. Experimental results indicate that the optimal conditions for this extraction were pH = 6.4, 1.42 mg of sorbent, 100 μL of eluent, and 0.84 mol L‐1 of eluent concentration. The detection limits are as low as 1.5, 0.3 and 0.9 μg L‐1 for lead, cadmium, and chromium, respectively. The intra‐day precisions were 3.6, 4.38 and 2.94 and Inter‐day precision were 4.83, 5.26 and 4.52 for lead, cadmium, and chromium, respectively. Method performance was investigated by determination of mentioned heavy metals in complicated biological matrixes such as human plasma, urine and saliva samples with good recoveries.
Optimization of solid phase dispersive field‐assisted ultrasonication for the extraction of auramine O and crystal violet dyes using central composite design
Authors: Pourebrahim Faezeh , Ghaedi Mehrorang , Dashtian Kheibar , Kheirandish Shadi , Goudarzi Alireza 
Published: December 2017

Simultaneous preconcentration and determination of auramine o (AO) and crystal violet (CV) dyes from aqueous solution was conducted by ultrasound assisted (dispersive) solid phase microextraction (UASPME) based on SnO2/SnS composite loaded activated carbon (SnO2/SnS‐NCs‐AC). The prepared of SnO2/SnS‐NCs‐AC was characterized by FESEM and XRD analysis. Main and interaction influences of operational parameters such as pH, sonication time, amounts of sorbent, and type of eluent on extraction efficiency were investigated by central composite design and optimized with desirability function approach (DFA). ANOVA was conducted and shows that optimized values were found at 15.33 min sonication time, 0.019 g SnO2/SnS‐NCs‐AC mass, pH 5.46 and among different solvents, dimethyl formamide was selected as an efficient eluent. Under this conditions recoverees percentage were obtained 82.85% and 86.70% for AO and CV, respectively. Based on F‐test under ANOVA all main effect and interaction effect of understudy parameters has the significant effect on the responses. At optimum conditions, limit of detection (0.0015 and 0.001 mg/l), limit of quantitation (0.4 and 0.4 mg/l), limit of linearity (9.0 and 9.0 mg/l), enrichment factor (33.48 and 83.71) and percent relative standard deviation (3.44 and 4.20) were found to be for auramine o and crystal violet dyes, respectively. Finally, the method was successfully applied for the preconcentration and determination of AO and CV in water samples and ER% of 89.0‐97.0 and 96.2–98.0% as acceptable range were found to be for AO and CV samples, respectively.
Visible light driven amide synthesis in water at room temperature from Thioacid and amine using CdS nanoparticles as heterogeneous Photocatalyst
Authors: Das Sudipto , Ray Shounak , Ghosh Abhisek Brata , Samanta Partha Kumar , Samanta Suvendu , Adhikary Bibhutosh , Biswas Papu 
Published: December 2017

Highly efficient photocatalytic thioacid mediated amide synthesis at room temperature using CdS nanoparticles as photocatalyst was observed under a household 30 W CFL in water. The operationally mild reaction was tolerant to a number of functional group substitutions on amine and could be scaled up to gram. This heterogeneous photocatalyst was extremely stable and could easily be recovered by simple centrifugation for at least six recycling reactions without any significant loss of catalytic performance. The possible reaction mechanism for the photocatalytic thioacid mediated amide synthesis over the CdS semiconductor has also been proposed on the basis of experimental observations.
Poly(2‐hydroxyethyl acrylate) hydrogels reinforced with graphene oxide: Remarkable improvement of water diffusion and mechanical properties
Authors: Sánchez‐Correa F. , Vidaurre‐Agut C. , Serrano‐Aroca Á. , Campillo‐Fernández A. J. 
Published: December 2017

ABSTRACTA series of hybrid hydrogels of poly(2‐hydroxyethyl acrylate), PHEA, and graphene oxide, GO, with GO content up to 2 wt % has been prepared by in situ polymerization. Because PHEA has been used as biomaterial in various applications, has a side chain with the hydroxyl functional group and its mechanical properties are poor, it is a good candidate for reinforcement with GO. Fourier transform (infrared) spectroscopy, atomic force microscopy, differential scanning calorimetry, the thermal, mechanical, and water sorption properties of neat PHEA and PHEA/GO composites have been studied in order to elucidate the dispersion and interaction between both components. An increase in the water diffusion coefficient and dramatic changes in its mechanical properties are the most remarkable results. Thus, at a nanofiller load of 2 wt %, the novel materials present an increased diffusion coefficient higher than 380% and the elastic modulus is enhanced by more than 650% in dry state and by more than 100% in swollen state, both compared to neat PHEA. These results have been attributed to the excellent interaction between the matrix, PHEA, and the reinforcement, GO, and could open the door to new applications in the field of biomaterials with higher structural requisites. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46158.
Preparation of active antibacterial biomaterials based on sparfloxacin, enrofloxacin, and lomefloxacin deposited on polyethylene
Authors: Ozaltin Kadir , Lehocky Marian , Humpolicek Petr , Vesela Daniela , Mozetic Miran , Novak Igor , Saha Petr 
Published: December 2017

ABSTRACTBacterial contamination is one of the biggest drawbacks of implanted biomaterials, which may cause nosocomial infection. Avoiding bacterial adhesion onto the biomaterial surface by preparing active antibacterial biomaterials is an accurate solution. In this study, three of the fluoroquinolones: sparfloxacin, enrofloxacin, and lomefloxacin were selected due to their broad bactericidal effect and immobilized onto low‐density polyethylene surface at two different pH values (pH 3 and pH 6), after tailoring the surface by plasma treatment followed by grafting of polymer brush of N‐allylmethylamine. Surface wettability test and morphological investigations were carried out by water contact angle measurement and scanning electron microscopy analysis, respectively. Chemical characterizations were carried out by Fourier transform infrared spectra and X‐ray photoelectron spectroscopy. Antibacterial activity was checked against Staphylococcus aureus and Escherichia coli strains and results revealed that prepared surfaces were more effective against E. coli than S. aureus especially those prepared at pH 6. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46174.
In Situ Synthesis of 3D Flower‐Like Nanocrystalline Ni/C and its Effect on Hydrogen Storage Properties of LiAlH4
Authors: Zang Lei , Liu Song , Guo Huinan , Chang Xiaoya , Xu Xiangqian , Jiao Lifang , Yuan Huatang , Wang Yijing 
Published: December 2017

AbstractLithium alanate (LiAlH4) is of particular interest as one of the most promising candidates for solid‐state hydrogen storage. Unfortunately, high dehydrogenation temperatures and relatively slow kinetics limit its practical applications. Herein, 3D flower‐like nanocrystalline Ni/C, composed of highly dispersed Ni nanoparticles and interlaced carbon flakes, was synthesized in situ. The as‐synthesized nanocrystalline Ni/C significantly decreased the dehydrogenation temperature and dramatically improved the dehydrogenation kinetics of LiAlH4. It was found that the LiAlH4 sample with 10 wt % Ni/C (LiAlH4‐10 wt %Ni/C) began hydrogen desorption at approximately 48 °C, which is very close to ambient temperature. Approximately 6.3 wt % H2 was released from LiAlH4‐10 wt %Ni/C within 60 min at 140 °C, whereas pristine LiAlH4 only released 0.52 wt % H2 under identical conditions. More importantly, the dehydrogenated products can partially rehydrogenate at 300 °C under 4 MPa H2. The synergetic effect of the flower‐like carbon substrate and Ni active species contributes to the significantly reduced dehydrogenation temperatures and improved kinetics.
Stem cell education for medical students at Tongji University: Primary cell culture and directional differentiation of rat bone marrow mesenchymal stem cells
Authors: Jin Caixia , Tian Haibin , Li Jiao , Jia Song , Li Siguang , Xu Guo‐Tong , Xu Lei , Lu Lixia 
Published: December 2017

AbstractStem cells are cells that can self‐renew and differentiate into a variety of cell types under certain conditions. Stem cells have great potential in regenerative medicine and cell therapy for the treatment of certain diseases. To deliver knowledge about this frontier in science and technology to medical undergraduate students, we designed an innovative practical experiment for freshmen in their second semester. The lab exercise focused on rat bone marrow mesenchymal stem cell (BMSC) isolation, cell culture and differentiation, and it aimed to help students master the aseptic techniques for cell culture, the basic methods and procedures for the primary culture and passage of BMSCs, the basic procedure for the directional differentiation of BMSCs into adipocytes and their subsequent identification by oil‐red‐O staining. This lab exercise is a very meaningful and useful introduction to stem cell collection and manipulation and inspires medical students to deepen their understanding of translational medicine and regenerative medicine. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(2):151–154, 2018.
Small laccase from streptomyces coelicolor—an ideal model protein/enzyme for undergraduate laboratory experience
Authors: Cook Ryan , Hannon Drew , Southard Jonathan N. , Majumdar Sudipta 
Published: December 2017

AbstractA one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and purification, and protein analysis and characterization. Students perform the tasks of cloning, expression, purification, analysis, and characterization of small laccase (SLAC) from Streptomyces coelicolor. SLAC is an extremely robust well‐characterized protein/enzyme, which serves as an ideal model for undergraduate teaching laboratories. Also, this goal‐oriented research‐like approach helps students to unite the concepts of biochemistry and molecular biology and appreciate the utility of the methods more effectively. A student assessment before and after the course demonstrated an overall increase in learning and enthusiasm on the topic of modern protein chemistry. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(2):172–181, 2018.
Nine‐month results of the BIOHELIX‐I clinical trial study: Evaluation of the PRO‐Kinetic Energy cobalt chromium bare‐metal stent system
Authors: Michael Tesfaldet T. , Richardt Gert , Lansky Alexandra , Carney Robert J. , Khan Muhammad Akram , Shehadeh Abbas , Zeymer Uwe , Gupta Saurabh 
Published: December 2017

AbstractObjectivesTo evaluate the safety and efficacy of the PRO‐Kinetic Energy (PKE) Cobalt Chromium Coronary Stent System (BIOTRONIK AG, Switzerland).BackgroundPercutaneous coronary intervention is a mainstay treatment for symptomatic coronary artery disease (CAD). While drug‐eluting stents constitute a majority of implants, bare‐metal stents (BMS) remain important for a subset of patients. Newer generation BMS offer advantages due to stent design improvements.MethodsThe BIOHELIX‐I study was a prospective, multicenter, non‐randomized, single arm clinical trial designed to evaluate the safety and efficacy of the PKE bare metal stent. Thirty‐three study centers (US, Columbia, Europe) enrolled 329 patients for treatment of one target lesion (≤31 mm). Eligible patients received a PKE stent(s), at least one month of dual antiplatelet therapy and were followed for 36‐months. The primary endpoint was the 9‐month rate of target vessel failure (TVF) compared with a prespecified performance goal of 18.7% derived from prior BMS trials.ResultsThe mean patient age was 69 years, 28.6% with diabetes. The mean lesion length was 13.7 ± 6.0 mm. The 9‐month TVF rate was 9.06% and met the primary endpoint (P < 0.001). The TVF component rates were 0.95% cardiac death, 1.58% myocardial infarction, and 7.26% ischemia‐driven target vessel revascularization (TVR). The ischemia‐driven target lesion revascularization rate at 9‐months was 6.62%.ConclusionsThe 9‐month TVF rate of the PKE stent was comparable to other BMS and is a viable option for treating CAD. The low observed rate of ischemia‐driven TVR supports the safety and efficacy of the novel BMS design.
Photovoltaic Devices Prepared through a Trihydroxy Substitution Strategy on an Unsymmetrical Squaraine Dye
Authors: Wu Jianglin , Si Changfeng , Chen Yao , Yang Lin , Hu Bin , Chen Guo , Lu Zhiyun , Huang Yan 
Published: December 2017

AbstractA series of unsymmetrical arene‐1,3‐squaraine (USQ) derivatives with two, three, or four hydroxy (−OH) substituents, namely, USQ‐2‐OH, USQ‐3‐OH, or USQ‐4‐OH, respectively, were designed and synthesized, and the effect of the number of hydroxy groups on the optoelectronic properties of USQs were investigated. Despite the three compounds having similar UV/Vis absorption and HOMO energy levels, solution‐processed bulk‐heterojunction (BHJ) small‐molecule organic solar cells with USQ‐3‐OH as electron‐donor materials exhibit the highest power conversion efficiency of 6.07 %, which could be mainly attributed to the higher hole mobility and smaller phase separation. It is also noteworthy that the short‐circuit current (Jsc) of the USQ‐3‐OH‐based device is as high as 14.95 mA cm−2, which is the highest Jsc values reported for squaraine‐based BHJ solar cells to date. The results also indicate that more −OH substituents on squaraine dyes do not necessarily lead to better photovoltaic performance.
Enantiomeric separation of type I and type II pyrethroid insecticides with different chiral stationary phases by reversed‐phase high‐performance liquid chromatography
Authors: Zhang Ping , Yu Qian , He Xiulong , Qian Kun , Xiao Wei , Xu Zhifeng , Li Tian , He Lin 
Published: December 2017

AbstractThe enantiomeric separation of type I (bifenthrin, BF) and type II (lambda‐cyhalothrin, LCT) pyrethroid insecticides on Lux Cellulose‐1, Lux Cellulose‐3, and Chiralpak IC chiral columns was investigated by reversed‐phase high‐performance liquid chromatography. Methanol/water or acetonitrile/water was used as mobile phase at a flow rate of 0.8 mL/min. The effects of chiral stationary phase, mobile phase composition, column temperature, and thermodynamic parameters on enantiomer separation were carefully studied. Bifenthrin got a partial separation on Lux Cellulose‐1 column and baseline separation on Lux Cellulose‐3 column, while LCT enantiomers could be completely separated on both Lux Cellulose‐1 and Lux Cellulose‐3 columns. Chiralpak IC provided no separation ability for both BF and LCT. Retention factor (k) and selectivity factor (α) decreased with the column temperature increasing from 10°C to 40°C for both BF and LCT enantiomers. Thermodynamic parameters including ∆H and ∆S were also calculated, and the maximum Rs were not always obtained at lowest temperature. Furthermore, the quantitative analysis methods for BF and LCT enantiomers in soil and water were also established. Such results provide a new approach for pyrethroid separation under reversed‐phase condition and contribute to environmental risk assessment of pyrethroids at enantiomer level.
The association of low muscle mass with soluble receptor for advanced glycation end products (sRAGE): The Korean Sarcopenic Obesity Study (KSOS)
Authors: Kim Tae Nyun , Park Man Sik , Lee Eun Joo , Chung Hye Soo , Yoo Hye Jin , Kang Hyun Joo , Song Wook , Baik Sei Hyun , Choi Kyung Mook 
Published: December 2017

AbstractBackgroundAdvanced glycation end products (AGEs) are accumulated with aging in various tissues of humans. The soluble receptor for AGEs (sRAGE) exerts a protective role against the development of aging‐related chronic disorders by neutralizing the action of AGEs. We investigated the implication of sRAGE on low muscle mass in Asian men and women.MethodsThis cross‐sectional study included a 390‐participant, nondiabetic subcohort recruited within the framework of the Korean Sarcopenic Obesity Study, an ongoing prospective cohort study. Low muscle mass was defined based on the distribution of appendicular skeletal muscle mass divided by body mass index, as proposed by the Foundation for the National Institutes Sarcopenia Project.ResultsSerum sRAGE levels were significantly lower in participants with low muscle mass than in participants without low muscle mass (0.76 [0.60‐1.00] ng/mL vs 0.87 [0.67‐1.15] ng/mL, P = .005). In age‐ and sex‐adjusted correlation analyses, appendicular skeletal muscle mass divided by body mass index was associated with sRAGE (r = 0.109, P = .037). Furthermore, decreased circulating levels of sRAGE are independently associated with low muscle mass (odds ratio = 0.254, P = .002) after adjusting for confounding factors, including insulin resistance and inflammatory markers.ConclusionsThe present study shows that a low circulating level of sRAGE may be an independent risk factor for the presence of low muscle mass.