bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential protein expression marks the transition from infection withOpisthorchis viverrinito cholangiocarcinoma
Authors: Khoontawad Jarinya , Pairojkul Chawalit , Rucksaken Rucksak , Pinlaor Porntip , Wongkham Chaisiri , Yongvanit Puangrat , Pugkhem Ake , Jones Alun , Plieskatt Jordan , Potriquet Jeremy , Bethony Jeffery , Pinlaor Somchai , Mulvenna Jason 
Published: November 2016

SummaryParts of Southeast Asia have the highest incidence of intrahepatic cholangiocarcinoma (CCA) in the world due to infection by the liver flukeOpisthorchis viverrini(Ov). Ov-associated CCA is the culmination of chronic Ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in Ov-infected individuals prior to transitioning to CCA. Isobaric labelling and tandem mass spectrometry of liver tissue from a hamster model of CCA was used to compare protein expression profiles from inflammed tissue (Ov-infected but not cancerous) versus cancerous tissue (Ov-induced CCA). Immunohistochemistry and immunoblotting were used to verify dysregulated proteins in the animal model and in human tissue. We identified 154 dysregulated proteins that marked the transition from Ov-infection to Ov-induced CCA, i.e. proteins dysregulated during carcinogenesis but not Ov-infection. The verification of dysregulated proteins in resected liver tissue from humans with Ov-associated CCA showed the numerous parallels in protein dysregulation between human and animal models of Ov-induced CCA. To identify potential circulating markers for CCA, dysregulated proteins were compared to proteins isolated from exosomes secreted by a human CCA cell line (KKU055) and 27 proteins were identified as dysregulated in CCA and present in exosomes. These data form the basis of potential diagnostic biomarkers for human Ov-associated CCA. The profile of protein dysregulation observed during chronic Ov-infection and then in Ov-induced CCA provides insight into the etiology of an infection-induced inflammation-related cancer.AbbreviationsCCAcholangiocarcinomaOvOpisthorchis viverriniicNDMAN -nitrosodimethylamineIHCimmunohistochemistryMMTSmethyl methanethiosulfonateTPPTrans Proteomic Pipeline
Cis-regulatory divergence in gene expression between two thermally divergent yeast species
Authors: Li Xueying C. , Fay Justin C. 
Published: November 2016

AbstractGene regulation is a ubiquitous mechanism by which organisms respond to their environment. While organisms are often found to be adapted to the environments they experience, the role of gene regulation in environmental adaptation is not often known. In this study, we examine divergence incis-regulatory effects between twoSaccharomycesspecies,S. cerevisiaeandS. uvarum, that have substantially diverged in their thermal growth profile. We measured allele specific expression (ASE) in the species’ hybrid at three temperatures, the highest of which is lethal toS. uvarumbut not the hybrid orS. cerevisiae. We find thatS. uvarumalleles can be expressed at the same level asS. cerevisiaealleles at high temperature and mostcis-acting differences in gene expression are not dependent on temperature. While a small set of 136 genes show temperature-dependent ASE, we find no indication that signatures of directionalcis-regulatory evolution are associated with temperature. Within promoter regions we find binding sites enriched upstream of temperature responsive genes, but only weak correlations between binding site and expression divergence. Our results indicate that temperature divergence betweenS. cerevisiaeandS. uvarumhas not caused widespread divergence incis-regulatory activity, but point to a small subset of genes where the species’ alleles show differences in magnitude or opposite responses to temperature. The difficulty of explaining divergence incis-regulatory sequences with models of transcription factor binding sites and nucleosome positioning highlights the importance of identifying mutations that underliecis-regulatory divergence between species.
Inactivation of thermogenic UCP1 as a historical contingency in multiple placental mammal clades
Authors: Gaudry Michael J. , Jastroch Martin , Treberg Jason R. , Hofreiter Michael , Paijmans Johanna L.A. , Starrett James , Wales Nathan , Signore Anthony V. , Springer Mark S. , Campbell Kevin L. 
Published: November 2016

ABSTRACTMitochondrial uncoupling protein 1 (UCP1) is essential for non-shivering thermogenesis in brown adipose tissue and is widely accepted to have played a key thermoregulatory role in small-bodied and neonatal placental mammals that enabled the exploitation of cold environments. Here we mapucp1sequences from 133 mammals onto a species tree constructed from a ∼51-kb sequence alignment and show that inactivating mutations have occurred in at least eight of the 18 traditional placental orders, thereby challenging the physiological importance of UCP1 across Placentalia. Selection and timetree analyses further reveal thatucp1inactivations temporally correspond with strong secondary reductions in metabolic intensity in xenarthrans and pangolins, or in six other lineages coincided with a ∼30 million year episode of global cooling in the Paleogene that promoted sharp increases in body mass and cladogenesis evident in the fossil record. Our findings also demonstrate that members of various lineages (e.g., cetaceans, horses, woolly mammoths, Steller’s sea cows) evolved extreme cold hardiness in the absence of UCP1-mediated thermogenesis. Finally, we identifyucp1inactivation as a historical contingency that is linked to the current low species diversity of clades lacking functional UCP1, thus providing the first evidence for species selection related to the presence or absence of a single gene product.
Water-soluble thiosemicarbazide-imidazole derivative as an efficient inhibitor protecting P110 carbon steel from CO2 corrosion
Authors: He Yi , Yang Ranran , Zhou Yanqiu , Ma Lan , Zhang Lei , Chen Zhao 
Published: November 2016

Purpose
The purpose of this paper is to adopt both the fragments of imidazole and thiosemicarbazide as reaction centers, to synthesize a water-soluble corrosion inhibitor named as thiosemicarbazide-imidazole derivative (TH-IM).

Design/methodology/approach
This paper presents potentiodynamic polarization tests, electrochemical impedance spectroscopy tests and morphology study of metal surface by scanning electron microscope and thermodynamic study.

Findings
In the aggressive condition of CO2 saturated Wt. NaCl solution at 333 K for 72 h with 400 ppm inhibitor dosage, the efficiency of inhibition increases approximately 98 per cent through the method of weight loss.

Originality/value
This paper finds a new corrosion inhibitor about TH-IM which has a good solubility in water.
Characterizing biofilms for biofouling and microbial corrosion control in cooling water systems
Authors: George R.P. , Kamachi Mudali U. , Raj Baldev 
Published: November 2016

Purpose
The purpose of this paper is to study the metal-Microbe interaction playing a crucial role in microbiologically influenced corrosion (MIC) and biofouling of materials in cooling water systems. Treatment regimens should be planned based on this understanding.

Design/methodology/approach
Attempts were made in the past decades to characterize and understand biofilm formation on important power plant structural materials such as carbon steel (CS), stainless steel (SS) and titanium in fresh water and in seawater to achieve better control of biofouling and minimize MIC problems.

Findings
This report presents the results of detailed studies on tuberculation-formed CS because of the action of iron-oxidizing bacteria and the effects of algae- and bacteria-dominated biofilms on the passivity of SS. The preferential adhesion of different bacterial species on SS under the influence of inclusions and sensitization was studied in the context of preferential corrosion of SS weldments due to microbial action. Detailed characterization of biofilms formed on titanium (the likely condenser material for fast breeder reactors) after exposure for two years in Kalpakkam coastal waters revealed intense biofouling and biomineralization of manganese even in chlorinated seawater. Studies on the effectiveness of conventional fouling control strategies were also evaluated.

Originality/value
The detailed studies of different metal/biofilm/microbe interactions demonstrated the physiological diversity of microbes in the biofilms that were formed on different materials, coupling their cooperative metabolic activities with consequent corrosion behaviour. These interactions could enhance either anodic or cathodic reactions and exploit metallurgical features that enhance biofilm formation and/or the capacity of microbes to mutate and overcome mitigation measures.
Corrosion behavior of low pressure cold sprayed Zn-Ni composites coating
Authors: Huang Guosheng , Li Xiangbo , Xing Lukuo 
Published: November 2016

PurposeThis paper aims to examine the performance of low-pressure cold-sprayed zinc–nickel (Zn-Ni) composites coating, i.e. whether it has the same performance as Zn-Ni alloy coating.Design/methodology/approachIn this paper, Zn-Ni composites coatings containing four different nickel contents were prepared with commercial DYMET 413 low-pressure cold spraying system under the same parameters. Corrosion behaviors of four kinds of coatings were examined with potentiodynamic polarization curves and electrochemical impedance spectroscopy methods, combined with scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction.FindingsCorrosion behavior of Zn-Ni composites coating is similar to Zn-Ni alloy coating. In the early stages of immersion, the anodic dissolution of zinc happens, which results in the formation of a zinc hydroxide layer. With the continuous infiltration of chloride ion, zinc hydroxide will get converted to zinc oxide, basic zinc chloride and basic zinc carbonate. The presence of nickel in coatings can prevent zinc hydroxide from converting into zinc oxide.Research limitations/implicationsFurther research should be done on improving the deposition efficiency, as the deposition efficiency of low-pressure cold spray is lower than 30 per cent.Practical implicationsLow-pressure cold spray coating can be used in cyclic dry/wet conditions to prolong the life of a steel structure.Social implicationsLow-pressure cold spray Zn-Ni coating is an environmentally friendly anticorrosion method which can be used as an alternative of hexavalent chromium passivation coating.Originality/valueZn-Ni composite coating can be deposited on steel directly by low-pressure cold spray by mechanically mixing the powders together. The composite coating also has the same long-term anticorrosion performance as Zn-Ni alloy coating.
Creating micro-videos to demonstrate technology learning and digital literacy
Authors: Frydenberg Mark , Andone Diana 
Published: November 2016

Purpose
Short videos, also known as micro-videos, have emerged as a platform for sharing ideas, experiences and life events via online social networks. This paper aims to share preliminary results of a study, involving students from two universities who created six-second videos using the Vine mobile app to explain or illustrate technological concepts.

Design/methodology/approach
Students were assigned the task of creating a Vine video to demonstrate their understanding of an assigned technological concept. A survey, performed after creating their micro-videos, asked students about the technology tools they use that allow them to be creative, their current personal digital literacy skills and the extent to which they are able to express a complex concept in a simple way. The study categorizes the students’ micro-videos to determine which formats might be most effective in demonstrating technology learning.

Findings
An analysis of their videos shows that the six-second constraint often inspires creativity and critical thinking, as students need to carefully consider the message they wish to convey, and how they can do so effectively in a compelling micro-video. The creation of such videos provides an effective way to demonstrate student learning and digital literacy skills.

Research limitations/implications
The study presents preliminary results gathered during one semester, with 68 videos created by 75 students.

Practical implications
Micro-video platforms are popular among today’s students, whose increased use of social media, dependence on mobile devices and desire for constant connectivity enable new outlets for creativity and communication. This study examines the introduction of a micro-video platform, which many students already use in their personal lives, in an educational context to develop their technology and digital literacy skills.

Social implications
Students learn to use an emerging social media platform as a vehicle to inspire creativity and learning.

Originality/value
This study explores and shares preliminary results related to the use of micro-videos for demonstrating technology learning and developing digital literacy skills in the information technology classroom.
Differentiating customer engaging behavior by targeted benefits – an empirical study
Authors: Braun Corina , Batt Verena , Bruhn Manfred , Hadwich Karsten 
Published: November 2016

Purpose
Relationship marketing scholars and managers have recognized the potential of customer engagement to enhance business performance and customer value. Therefore, the purpose of this paper is to examine the effects that different types of customer engagement behaviors have on their perceived benefits.

Design/methodology/approach
The authors conducted two empirical studies. In the first step, 69 in-depth interviews were held to identify important customer engagement behaviors and targeted benefits. Then, in the second step, a quantitative study with 255 participants was used to match the identified customer engagement behaviors with the targeted benefits.

Findings
The results reveal that there are three aggregated types of customer engagement behaviors (“value creation-focused customer engagement”, “online-focused customer engagement” and “customer-to-customer interaction-focused customer engagement”). These types of customer engagement behaviors lead to different targeted benefits (social, relationship, autonomous, economic, altruistic and self-fulfillment benefits).

Research limitations/implications
A consideration of the influencing factors of the different customer-engagement-behavior types, including customers’ motives for their engagement with a company, would potentially enhance the findings. Furthermore, a closer investigation of the relationship between socio-demographic characteristics and customer engagement types can also provide deeper insights into the reasons for engaging with a certain firm or brand.

Practical implications
The findings provide managers with information on how to segment customers according to their customer engagement type and associated benefits and thereby enable them to manage customer engagement behaviors more profitably.

Originality/value
The results make a key contribution to the emerging research field of customer engagement by gaining deeper insights into the benefits associated with different customer engagement behaviors. It becomes clear that different customer engagement types aim at receiving various benefits.
GREEN consumption values and Indian consumers’ response to marketing communications
Authors: Bailey Ainsworth Anthony , Mishra Aditya , Tiamiyu Mojisola F. 
Published: November 2016

Purpose
This paper aims to report on a study that assessed Indian consumers’ response to green marketing communications, based on their GREEN consumption values. GREEN (Haws et al., 2014) refers to consumers’ tendency to express their environmental concern through their consumption behaviors. This study applies this construct in a marketing communications context.

Design/methodology/approach
Two conceptual models involving GREEN were developed, and data to test the models were collected in a survey conducted among a convenience sample of 284 Indian consumers.

Findings
The results show that GREEN can enhance understanding of consumers’ green attitudes and intentions. GREEN consumption values have an impact on how Indian consumers respond to advertising and public relations stimuli, as GREEN influences perceptions of green brand trust, attitudes toward green marketing communications and green brand support and purchase intentions.

Research limitations/implications
The research adds to the growing literature on green marketing in emerging economies and extends the application of the GREEN construct from the domain of consumer behavior to that of green marketing communications.

Practical implications
The results suggest that marketers should focus on developing green marketing communications strategy, rather than just green advertising strategies, and they can position their green products to appeal to consumers based on GREEN consumption values.

Originality/value
The study is the first to apply the GREEN construct in assessing consumer response to a brand’s green marketing communications; it also explores this issue in an emerging economy.
Integrating alternative technologies to improve built environment sustainability in Africa
Authors: GhaffarianHoseini Ali , Tookey John , GhaffarianHoseini Amirhosein , Naismith Nicola , Bamidele Rotimi James Olabode 
Published: November 2016

PurposeThe purpose of this paper is to review extant literature and to provide perspectives on approaches to enhancing built environment sustainability in Africa. There is a mismatch between global societal resources and the increasing demand for natural resources. The consequences of this mismatch are prevalent in many African countries, causing the need to implement of built environment sustainability as a matter of cause.Design/methodology/approachLittle research has been undertaken to date with a focus on the environmental sustainability of Africa. With this in mind the review was undertaken through a series of incremental steps. It began with an initial review, before developing through exploratory and development phases. The process culminated with the refined literature review presented.FindingsThe paper finds that a different approach is required to achieve built sustainable development for developed and developing countries, with a clear difference in terms of its application observed between the Northern and Southern hemispheres. Current energy and water crisis facing Africa is brought to the fore and an evaluation is provided of the systems being used to ameliorate its effects. The study explores a range of technological solutions that are appropriate for consideration in the African context. It also examines the barriers that need to be overcome to facilitate the widespread use of the suggested solutions in Africa.Originality/valueThis study examines built environment sustainability through the Africa lens. It highlights its importance and the contextual factors inhibiting the widespread uptake of built environment sustainability solutions. The study offers a number of recommendations for the future to encourage long-term built environment sustainability in Africa and more specifically the Sub-Saharan region.
Extent of incorporation of green features in office properties in Lagos, Nigeria
Authors: Komolafe Markson Opeyemi , Oyewole Matthew Oluwole , Kolawole John Temitope 
Published: November 2016

Purpose
The purpose of this paper is to investigate the extent to which green building features are evident in office properties in Lagos, Nigeria; and consequently determine the degree of compliance with green standards in the country.

Design/methodology/approach
The study purposively sampled two (2) office properties from the management portfolio of 88 registered Estate firms in Lagos. Data were collected using physical observation on the properties and interview with two users purposively selected from each of the properties. The data were analysed with the use of frequency distribution, percentages and measures of green features availability index.

Findings
The result revealed a low extent of green features incorporation in existing office properties with the value of availability indices on most features falling below 2.5 on a five-point scale. Feature relating to material use and conservation is the most incorporated green feature (mean score of 2.62) while those relating to owner and occupant education were least in incorporation (mean score of 1.895).

Practical implications
From the findings, it is apparent that green retrofitting may be necessary in Nigeria due to the low extent of green practices in existing office properties. Also, reinforcement of existing government policies and increased sensitisation of stakeholders on impact of current building practices are pertinent to green building success in Nigeria.

Originality/value
Most existing studies of similar focus are based in the developed economies where stronger implementation framework exists for green building. Besides, they are mostly based on evaluation of green certified buildings using few criteria. This study differs in that it presents the existing building sustainability practices in a less pronounced green property market, with varying architectural styles using more robust criteria. Information provided is applicable in Nigeria and other emerging economies.
Supplemental Insurance and Racial Health Disparities under Medicare Part B
Authors: Brunt Christopher S. 
Published: November 2016

ObjectiveTo assess the impact of preferences, socioeconomic status (SES), and supplemental insurance (SI) on racial/ethnic disparities in the probability and use of services at physicians' offices, hospitals, and emergency departments among Medicare beneficiaries enrolled in Part B.Research Design and SubjectsThis study includes black and white beneficiaries from the 2009–2011 panel of the Medicare Current Beneficiary Survey who were enrolled in Medicare Part B. Logit and negative binomial multivariate regression analysis were used in conjunction with rank‐and‐replace methods to determine factors influencing utilization and black–white utilization disparities.Principal FindingsAmong Part B beneficiaries, significant disparities exist for each studied service. Examining contributing factors, 12–19 percent of the black–white health‐adjusted difference in the probability of use is explained by differences in SES, whereas differences in the distribution of SI accounts for 20 percent or more. For volume, SES is found to account for 2–11 percent of differences with SI making up another 9–10 percent.ConclusionsA substantial portion of the difference in black–white beneficiary use of outpatient services is due to SI. Policies aimed at increasing coverage are likely to increase the probability of visits with modest increases in volume.
Emulsion Stabilized with Starch Octenyl Succinate Prolongs Nisin Activity Against Listeria Monocytogenes in a Cantaloupe Juice Model
Authors: Sarkar Preetam , Bhunia Arun K. , Yao Yuan 
Published: November 2016

AbstractFood safety related to fresh and fresh‐cut produce, such as cantaloupe, is a great challenge in the food industry. Various processing technologies, including the use of antimicrobial materials, have been applied to reduce pathogens. Nisin, an antimicrobial peptide, shows strong inhibitory activity against Listeria monocytogenes (LM); however, it suffers from quick depletion in foods. In this study, the protective effect of starch octenyl succinate (starch‐OS) stabilized emulsion on nisin was evaluated in a cantaloupe juice model. Results showed that the proteolytic enzyme in cantaloupe juice caused nisin depletion, and the adsorption of nisin in emulsion led to considerable protection of antimicrobial activity. After 1, 3, and 6 d of incorporation with cantaloupe juice, the size of LM inhibitory ring were 7.64, 7.60, and 2.97 mm, respectively for emulsion formulations, whereas it reduced from 3.60 mm to a negligible level for nisin‐alone. This study showed the potential of using carbohydrate colloidal systems to prolong the efficacy of antimicrobial materials.