Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition
Authors: Alberti Adriana , Poulain Julie , Engelen Stefan , Labadie Karine , Romac Sarah , Ferrera Isabel , Albini Guillaume , Aury Jean-Marc , Belser Caroline , Bertrand Alexis , Cruaud Corinne , Da Silva Corinne , Dossat Carole , Gavory Frédérick , Gas Shahinaz , Guy Julie , Haquelle Maud , Jacoby E'krame , Jaillon Olivier , Lemainque Arnaud , Pelletier Eric , Samson Gaëlle , Wessner Mark ,   Genoscope Technical Team, Bazire Pascal , Beluche Odette , Bertrand Laurie , Besnard-Gonnet Marielle , Bordelais Isabelle , Boutard Magali , Dubois Maria , Dumont Corinne , Ettedgui Evelyne , Fernandez Patricia , Garcia Espérance , Aiach Nathalie Giordanenco , Guerin Thomas , Hamon Chadia , Brun Elodie , Lebled Sandrine , Lenoble Patricia , Louesse Claudine , Mahieu Eric , Mairey Barbara , Martins Nathalie , Megret Catherine , Milani Claire , Muanga Jacqueline , Orvain Céline , Payen Emilie , Perroud Peggy , Petit Emmanuelle , Robert Dominique , Ronsin Murielle , Vacherie Benoit , Acinas Silvia G. , Royo-Llonch Marta , Cornejo-Castillo Francisco M. , Logares Ramiro , Fernández-Gómez Beatriz , Bowler Chris , Cochrane Guy , Amid Clara , Hoopen Petra Ten , De Vargas Colomban , Grimsley Nigel , Desgranges Elodie , Kandels-Lewis Stefanie , Ogata Hiroyuki , Poulton Nicole , Sieracki Michael E. , Stepanauskas Ramunas , Sullivan Matthew B. , Brum Jennifer R. , Duhaime Melissa B. , Poulos Bonnie T. , Hurwitz Bonnie L. ,   Tara Oceans Consortium Coordinators, Acinas Silvia G. , Bork Peer , Boss Emmanuel , Bowler Chris , De Vargas Colomban , Follows Michael , Gorsky Gabriel , Grimsley Nigel , Hingamp Pascal , Iudicone Daniele , Jaillon Olivier , Kandels-Lewis Stefanie , Karp-Boss Lee , Karsenti Eric , Not Fabrice , Ogata Hiroyuki , Pesant Stéphane , Raes Jeroen , Sardet Christian , Sieracki Michael E. , Speich Sabrina , Stemmann Lars , Sullivan Matthew B. , Sunagawa Shinichi , Wincker Patrick , Pesant Stéphane , Karsenti Eric , Wincker Patrick 
Published: August 2017

AbstractA unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009–2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world’s planktonic ecosystems.