bluuebunny/crossref_metadata_2025_split huggingface dataset:
Localisation of Nup153 and SENP1 to nuclear pore complexes is required for 53BP1-mediated DNA double-strand break repair
Authors: Duheron Vincent , Nilles Nadine , Pecenko Sylvia , Martinelli Valérie , Fahrenkrog Birthe 
Published: June 2017

ABSTRACT
The nuclear basket of nuclear pore complexes (NPCs) is composed of three nucleoporins: Nup153, Nup50 and Tpr. Nup153 has a role in DNA double-strand break (DSB) repair by promoting nuclear import of 53BP1 (also known as TP53BP1), a mediator of the DNA damage response. Here, we provide evidence that loss of Nup153 compromises 53BP1 sumoylation, a prerequisite for efficient accumulation of 53BP1 at DSBs. Depletion of Nup153 resulted in reduced SUMO1 modification of 53BP1 and the displacement of the SUMO protease SENP1 from NPCs. Artificial tethering of SENP1 to NPCs restored non-homologous end joining (NHEJ) in the absence of Nup153 and re-established 53BP1 sumoylation. Furthermore, Nup50 and Tpr, the two other nuclear basket nucleoporins, also contribute to proper DSB repair, in a manner distinct from Nup153. Similar to the role of Nup153, Tpr is implicated in NHEJ and homologous recombination (HR), whereas loss of Nup50 only affects NHEJ. Despite the requirement of all three nucleoporins for accurate NHEJ, only Nup153 is needed for proper nuclear import of 53BP1 and SENP1-dependent sumoylation of 53BP1. Our data support the role of Nup153 as an important regulator of 53BP1 activity and efficient NHEJ.
The controversial Linnaean name Statice minuta (Plumbaginaceae)
Authors: Iamonico Duilio , Guacchio Emanuele Del 
Published: June 2017

AbstractThe original circumscription and nomenclatural history of Statice minuta are discussed. The taxon nowadays indicated by this name is endemic to the Baleares (Spain, western Mediterranean), but neither the lectotype nor the Linnaean protologue support this usage. The paper clarifies the origin of this mistake and preludes to a formal proposal to conserve the name with a conserved type.
Report of the General Committee: 18
Authors: Wilson Karen L. 
Published: June 2017

SummaryDecisions are reported on proposals and requests in Reports 15 and 17 of the Nomenclature Committee for Algae, Report 69 from the Nomenclature Committee for Vascular Plants, Reports 20 and 21 from the Nomenclature Committee for Fungi, plus an old proposal (1193) from the then Committee for Spermatophyta. The first six lists of fungal names authorized under Art. 14.13 from fungal working groups are approved, as are two additions to the list of institutional votes for the Nomenclature Section of the XIX IBC.
Eros e Thanatos em “Tempos Sombrios”: aspectos da teoria pulsional em diálogo com Stefan Zweig e Hannah Arendt
Authors: Wels Érica Schlude 
Published: June 2017

O presente artigo reflete sobre aspectos ligados à propensão do homem pela destruição e domínio do outro por meio da violência. Nesse sentido, elencamos reflexões sobre os conceitos psicanalíticos de Eros e Thanatos, além de contribuições do pensamento de Hannah Arendt (1987; 1995; 1997), sobretudo o conceito de “Banalidade do Mal”; O mal concretizado pelo nazismo é monstruosamente banal e burocrático, ao mesmo tempo que eficiente e sistemático, levado a cabo por agentes superficiais (LECHTE, 2002). Buscar-se-á estabelecer um diálogo entre esse arcabouço teórico e três novelas do escritor austríaco Stefan Zweig, ele próprio uma vítima declaradamente pacifista do exílio imposto aos escritores judeus no período da Segunda Guerra: “A coleção invisível” (2015, [1924]), “O Livreiro Mendel” (1999, [1929]) e “Xadrez, uma novela” (2015, [1944]) têm a guerra como pano-de-fundo; O presente trabalho objetiva explorar aspectos da chamada “mitologia psicanalítica”, campo definido por Freud como obscuro. Além do caráter destruidor intrínseco à natureza humana, como postulado nos textos freudianos que abordam a guerra (1916; 1933, [1932]), destacamos o protótipo da pulsão de morte: compulsão à repetição e ao retorno do inorgânico, movimento regressivo de retorno a um estado anterior (ROUDINESCO; PLON, 1998). Ao explorar a teoria das pulsões em seu dualismo Eros/Thanatos, lançamos luzes sobre o que Arendt define como “Tempos sombrios”.
In Shadows and Darkness: being authentic in Céline and Hillman (“intimité des choses” and “pathologizing” )
Authors: Novaes Cláudio Cledson , Vrbata Aleš 
Published: June 2017

Aim of this paper is to demonstrate a proximity or even complementarity between James Hillman’s and Louis-Ferdinand Céline’s vision of human soul and human condition. Even though their cultural and intellectual context differed significantly and they both used very different forms of expression, they repeatedly invoked intimate dimension of human existence as permeated by somehow pathological, peripherial or dark aspects of being. Nevertheless, both of them shared deep interest in bottom-line dimension of being which they called “soul” and which they linked with death, darkness, weakness and which they associated with socially disapproved ways of being. Even though Hillman could be labeled as reformist and Céline as nihilist, for both of them modern society and its programming cut modern man off his deeper sense of meaningfulness or as Céline puts it in from “intimité des choses”. Questioning intellectual legacy of Enlightenment, both Céline and Hillman find soul of modern man as pathologized and threatened but at the same time as the very source of meaningfulness. Like that Hillman and Céline can be viewed not just as cultural critics but as actively deconstructing, questioning modern project and modern subjectivity
„Revolution“. Zur Karriere eines Begriffs in Großbritannien, 1688–1714
Authors: Niggemann Ulrich 
Published: June 2017

ZusammenfassungIm England der Jahre nach der Glorious Revolution war der Revolutionsbegriff nicht nur enorm präsent. Die Behauptung, der Begriff sei dort marginal gewesen und sei erst durch die Rezeption französischer Texte in die Diskussion eingebracht worden, dürfte kaum haltbar sein. Ganz offenkundig war der Begriff unmittelbar nach 1688 ein wesentlicher Bestandteil der innerenglischen politischen Debatten. Genauso wenig haltbar sind aber auch Versuche, den Revolutionsbegriff nach 1688 auf eine bestimmte Bedeutung festzulegen. Vielmehr zeichnet sich die Semantik des Begriffs in den Jahren nach der Revolution durch ein hohes Maß an Uneindeutigkeit und Heterogenität aus, durch ein Nebeneinander verschiedener Semantiken, ja durch einen regelrechten Kampf um die Füllung eines von unterschiedlichen Parteien anerkannten und in positiver Weise genutzten Begriffs. Weder gingen damit zyklische oder restaurative Konnotationen einfach verloren, noch lässt sich „Revolution“ im England des ausgehenden 17. und beginnenden 18. Jahrhunderts eindeutig auf die Konnotation von Widerstand oder Umsturz von unten festlegen. Dementsprechend wäre es zu einfach, eine geradlinige Entwicklung zum modernen Revolutionsbegriff anzunehmen. Vielmehr weist der Begriff eine Vielzahl paralleler und gleichzeitiger Konnotationen auf, die eng mit den konkurrierenden Interpretationen der Ereignisse von 1688/89 verbunden waren. Was aber deutlich wird, ist die positive Abgrenzung gegenüber Rebellion. Während „Rebellion“ stets einen illegitimen Akt des Widerstands meinte und zumeist auf die Ereignisse der Bürgerkrieg und des Regizids der Jahrhundertmitte verwies, war „Revolution“ ein positiv konnotierter Begriff, der fast immer einen legitimen Umbruch meinte, ganz gleich ob dieser als legitimer Widerstand oder als Eingriff Gottes in den Lauf der Geschichte interpretiert wurde. Für die Funktionsbestimmung des Revolutionsbegriffs in den englischen Debatten nach 1688 bedeutet dies: Der Begriff „Revolution“ diente vor allem dazu, die Ereignisse, die zur Flucht Jakobs II. und zur Krönung Wilhelms III. und Marias II. führten, in positiver Weise von den Geschehnissen zwischen 1642 und 1660 abzugrenzen. Der Revolutionsbegriff war also ganz eindeutig ein positiver Gegenbegriff zur „Rebellion“ gegen Karl I. Genau darin lag seine Attraktivität für Whigs und Tories gleichermaßen.
Überlegungen zu einer historiografischen Bildanalyse
Authors: Jäger Jens 
Published: June 2017

ZusammenfassungUnübersehbar und vielfältig sind die historiografischen Zugänge zu Bildern als Quelle. Methodisch war und ist Rainer Wohlfeils Überlegung einer Historischen Bildkunde, die er in den 1980er Jahren vorstellte, noch immer ein wichtiger Referenzpunkt. In enger Anlehnung an das kunsthistorische Verfahren der Ikonografie-Ikonologie nach Erwin Panofsky stellte Wohlfeil ein dreistufiges Verfahren vor, welches Bildinhalt und -bedeutung mit dem historischen Kontext eng verband. Kritiker bemängelten an dem Verfahren, dass es zu sehr auf die Frühe Neuzeit ausgerichtet sei, Mehrdeutigkeiten und Eigendynamik von Bildaussagen verneine. Es schenke der Medialität und der Ästhetik zu wenig Aufmerksamkeit und vereinnahme Bilder zu sehr „nur“ als Symptom für historische Prozesse. Diese Einwände finden sich auch generell gegen historiografische Bildanalysen. Die Debatte innerhalb der Geschichtswissenschaft um adäquate analytische Zugänge trägt den kritischen Hinweisen vielfach Rechnung und hat mit der Perspektive der „Visual History“ und Übernahmen von methodischen Zugängen benachbarter Disziplinen das Instrumentarium stets erweitert. Die Überlegung, bei der Analyse von Bildquellen zunächst einen „konventionellen Sinn“ zu ermitteln, versteht sich als Beitrag zu dieser Debatte. Auf Grundlage eines aus Sicht der Geschichtswissenschaft operationalisierbaren Bildbegriffs soll als konventioneller Sinn eines Bildes die je zeitgenössische, innerhalb eines konkreten Kontextes übliche Deutung von Bildaussagen gelten. Nebenbedeutungen werden hierbei nicht ausgeschlossen. Sie erschließen sich aus besonderen Kontexten des Bildgebrauchs. Ausgangspunkt ist der Bildinhalt und seine je konkrete mediale Form, wie sie als Quellenbefund vorliegt. Ein solches Vorgehen empfiehlt sich zwar generell, hilft aber vor allem bei kontextarmen Massenbildern, die gleichwohl die überwiegende Mehrzahl verfügbarer Bildquellen ausmachen.
Konstruierte Entzauberung
Authors: Nierop Jantine 
Published: June 2017

AbstractJürgen Ebach, Hans Martin Dober, and Albrecht Grözinger have argued for the appropriation of Walter Benjamin’s philosophy into homiletics. In this article, I first look at their proposal in all its diversity. I then turn to a striking shift in Benjamin’s philosophical development. Subsequently, I contend that the argument for such an appropriation of Benjamin can be sharpened even more by considering precisely this shift. The article concludes with my own proposal for appropriating Benjamin’s philosophy. It shows that Benjamin’s approach helps answer a central pastoral question in homiletics.
An Orthodox Model of Practical/Pastoral Theology
Authors: Porumb Razvan 
Published: June 2017

AbstractThis study explores the Orthodox vision of theology as holistic, where liturgical and sacramental life on the one hand, and social action and commitment on the other hand are inextricably connected. Theology appears as an inseparable whole and therefore, all theology is implicitly practical/pastoral. The study approaches the Orthodox response to the western perspective of pastoral theology as engagement of the entire community of the Church. The concept of
A Theory of Religious Praxis in India
Authors: Lazar Roy 
Published: June 2017

AbstractThis article introduces Practical Theology as an academic discipline by tracing down its historical development in the West that inspired its emergence in the Indian context. From a Cinderella of theology, this youngest discipline has emerged as a very important critical theory validating every branch of theology thanks to its innovative approaches and effective methodologies. Part I is laying the foundation to trace down the tenets of Indian Practical Theology in Part II.
Removal of U(VI) from aqueous solution using TiO2 modified β-zeolite
Authors: Peng Liu , Ni Yuan , Wei Xiong , Hanyu Wu , Duoqiang Pan , Wangsuo Wu 
Published: June 2017

Abstract
β-Zeolite was synthesized and modified with TiO2. The synthesized materials were characterized and used for removal of U(VI) from aqueous solutions. The influences of pH, contact time and temperature on U(VI) adsorption onto modified β-zeolite by TiO2 were studied by batch technique, and XPS was employed to analysed the experimental data. The dynamic process showed that the adsorption of U(VI) onto TiO2/β-zeolite matched the pseudo-second-order kinetics model, and the adsorption of U(VI) were significantly dependent on pH values. Through simulating the adsorption isotherms by Langmuir, Freundlich and Dubini–Radushkevich (D–R) models, it could be seen, respectively that the adsorption patterns of U(VI) onto TiO2/β-zeolite were mainly controlled by surface complexation, and the adsorption processes were endothermic and spontaneous. The modification of β-zeolite by TiO2 it shows a novel material for the removing of U(VI) from water environment for industrialized application.
Investigation of selenium compounds as targets for 76,77Br production using protons of energies up to 34 MeV
Authors: Hassan H. Ebrahim , El-Azony Khaled M. , Azzam Ahmed , Qaim Syed M. 
Published: June 2017

Abstract
Selenium compounds of Zn, Sn and Cu were prepared using a conventional sintering method and the phase composition of each compound was investigated using X-ray diffraction. The compounds prepared were tested under variable irradiation and separation conditions for 76,77Br production. The activity of 77Br was measured as a function of proton beam current within the range 2–15 μA and the thermal stability of the irradiated compound was investigated. The results showed that the compounds ZnSe and Cu2Se are thermally more stable in comparison to the SnSe. The effects of several factors, such as temperature, gas flow rate and separation time, were studied to obtain the maximum separation yield of radiobromine by the dry distillation method. The data showed that the compound Cu2Se is the most suitable target for proton irradiation to produce 76,77Br at energies up to 20 MeV. A simplified method was used to prepare also CuSe, which was tested in irradiations with intermediate energy protons of beam currents up to 10 μA. The data of a typical production run using 17 MeV protons and the Cu2Se target are reported.
Cold Fusion: Massive Karyotype Evolution in the Antarctic Bullhead NotothenNotothenia coriiceps
Authors: Amores Angel , Wilson Catherine A , Allard Corey A H , Detrich H William , Postlethwait John H 
Published: June 2017

AbstractHalf of all vertebrate species share a series of chromosome fusions that preceded the teleost genome duplication (TGD), but we do not understand the causative evolutionary mechanisms. The “Robertsonian-translocation hypothesis” suggests a regular fusion of each ancestral acro- or telocentric chromosome to just one other by centromere fusions, thus halving the karyotype. An alternative “genome-stirring hypothesis” posits haphazard and repeated fusions, inversions, and reciprocal and nonreciprocal translocations. To study large-scale karyotype reduction, we investigated the decrease of chromosome numbers in Antarctic notothenioid fish. Most notothenioids have 24 haploid chromosomes, but bullhead notothen (Notothenia coriiceps) has 11. To understand mechanisms, we made a RAD-tag meiotic map with ∼10,000 polymorphic markers. Comparative genomics aligned about a thousand orthologs of platyfish and stickleback genes along bullhead chromosomes. Results revealed that 9 of 11 bullhead chromosomes arose by fusion of just two ancestral chromosomes and two others by fusion of three ancestral chromosomes. All markers from each ancestral chromosome remained contiguous, implying no inversions across fusion borders. Karyotype comparisons support a history of: (1) Robertsonian fusions of 22 ancestral chromosomes in pairs to yield 11 fused plus two small unfused chromosomes, like N. angustata; (2) fusion of one of the remaining two ancestral chromosomes to a preexisting fused pair, giving 12 chromosomes like N. rossii; and (3) fusion of the remaining ancestral chromosome to another fused pair, giving 11 chromosomes in N. coriiceps. These results raise the question of what selective forces promoted the systematic fusion of chromosomes in pairs and the suppression of pericentric inversions in this lineage, and provide a model for chromosome fusions in stem teleosts.
Coordination of Heparan Sulfate Proteoglycans with Wnt Signaling To Control Cellular Migrations and Positioning inCaenorhabditis elegans
Authors: Saied-Santiago Kristian , Townley Robert A , Attonito John D , da Cunha Dayse S , Díaz-Balzac Carlos A , Tecle Eillen , Bülow Hannes E 
Published: June 2017

AbstractHeparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, morphology, and network connectivity. HSPGs function as cofactors for multiple signaling pathways, including the Wnt-signaling molecules and their Frizzled receptors. To investigate the functional interactions among the HSPG and Wnt networks, we conducted genetic analyses of each, and also between these networks using five cellular migrations in the nematode Caenorhabditis elegans. We find that HSPG core proteins act genetically in a combinatorial fashion dependent on the cellular contexts. Double mutant analyses reveal distinct redundancies among HSPGs for different migration events, and different cellular migrations require distinct heparan sulfate modification patterns. Our studies reveal that the transmembrane HSPG SDN-1/Syndecan functions within the migrating cell to promote cellular migrations, while the GPI-linked LON-2/Glypican functions cell nonautonomously to establish the final cellular position. Genetic analyses with the Wnt-signaling system show that (1) a given HSPG can act with different Wnts and Frizzled receptors, and that (2) a given Wnt/Frizzled pair acts with different HSPGs in a context-dependent manner. Lastly, we find that distinct HSPG and Wnt/Frizzled combinations serve separate functions to promote cellular migration and establish position of specific neurons. Our studies suggest that HSPGs use structurally diverse glycans in coordination with Wnt-signaling pathways to control multiple cellular behaviors, including cellular and axonal migrations and, cellular positioning.
Canalization by Selection ofde NovoInduced Mutations
Authors: Fanti Laura , Piacentini Lucia , Cappucci Ugo , Casale Assunta M , Pimpinelli Sergio 
Published: June 2017

AbstractWaddington elaborated the concepts of “canalization and assimilation” to explain how an apparently somatic, stress-induced variant...One of the most fascinating scientific problems, and a subject of intense debate, is that of the mechanisms of biological evolution. In this context, Waddington elaborated the concepts of “canalization and assimilation” to explain how an apparently somatic variant induced by stress could become heritable through the germline in Drosophila. He resolved this seemingly Lamarckian phenomenon by positing the existence of cryptic mutations that can be expressed and selected under stress. To investigate the relevance of such mechanisms, we performed experiments following the Waddington procedure, then isolated and fixed three phenotypic variants along with another induced mutation that was not preceded by any phenocopy. All the fixed mutations we looked at were actually generated de novo by DNA deletions or transposon insertions, highlighting a novel mechanism for the assimilation process. Our study shows that heat-shock stress produces both phenotypic variants and germline mutations, and suggests an alternative explanation to that of Waddington for the apparent assimilation of an acquired character. The selection of the variants, under stress, for a number of generations allows for the coselection of newly induced corresponding germline mutations, making the phenotypic variants appear heritable.
Genetics of Genome-Wide Recombination Rate Evolution in Mice from an Isolated Island
Authors: Wang Richard J , Payseur Bret A 
Published: June 2017

Abstract
Recombination rate is a heritable quantitative trait that evolves despite the fundamentally conserved role that recombination plays in meiosis. Differences in recombination rate can alter the landscape of the genome and the genetic diversity of populations. Yet our understanding of the genetic basis of recombination rate evolution in nature remains limited. We used wild house mice (Mus musculus domesticus) from Gough Island (GI), which diverged recently from their mainland counterparts, to characterize the genetics of recombination rate evolution. We quantified genome-wide autosomal recombination rates by immunofluorescence cytology in spermatocytes from 240 F2 males generated from intercrosses between GI-derived mice and the wild-derived inbred strain WSB/EiJ. We identified four quantitative trait loci (QTL) responsible for inter-F2 variation in this trait, the strongest of which had effects that opposed the direction of the parental trait differences. Candidate genes and mutations for these QTL were identified by overlapping the detected intervals with whole-genome sequencing data and publicly available transcriptomic profiles from spermatocytes. Combined with existing studies, our findings suggest that genome-wide recombination rate divergence is not directional and its evolution within and between subspecies proceeds from distinct genetic loci.
LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans
Authors: Tsukamoto Tatsuya , Gearhart Micah D , Spike Caroline A , Huelgas-Morales Gabriela , Mews Makaela , Boag Peter R , Beilharz Traude H , Greenstein David 
Published: June 2017

Abstract
An extended meiotic prophase is a hallmark of oogenesis. Hormonal signaling activates the CDK1/cyclin B kinase to promote oocyte meiotic maturation, which involves nuclear and cytoplasmic events. Nuclear maturation encompasses nuclear envelope breakdown, meiotic spindle assembly, and chromosome segregation. Cytoplasmic maturation involves major changes in oocyte protein translation and cytoplasmic organelles and is poorly understood. In the nematode Caenorhabditis elegans, sperm release the major sperm protein (MSP) hormone to promote oocyte growth and meiotic maturation. Large translational regulatory ribonucleoprotein (RNP) complexes containing the RNA-binding proteins OMA-1, OMA-2, and LIN-41 regulate meiotic maturation downstream of MSP signaling. To understand the control of translation during meiotic maturation, we purified LIN-41-containing RNPs and characterized their protein and RNA components. Protein constituents of LIN-41 RNPs include essential RNA-binding proteins, the GLD-2 cytoplasmic poly(A) polymerase, the CCR4-NOT deadenylase complex, and translation initiation factors. RNA sequencing defined messenger RNAs (mRNAs) associated with both LIN-41 and OMA-1, as well as sets of mRNAs associated with either LIN-41 or OMA-1. Genetic and genomic evidence suggests that GLD-2, which is a component of LIN-41 RNPs, stimulates the efficient translation of many LIN-41-associated transcripts. We analyzed the translational regulation of two transcripts specifically associated with LIN-41 which encode the RNA regulators SPN-4 and MEG-1. We found that LIN-41 represses translation of spn-4 and meg-1, whereas OMA-1 and OMA-2 promote their expression. Upon their synthesis, SPN-4 and MEG-1 assemble into LIN-41 RNPs prior to their functions in the embryo. This study defines a translational repression-to-activation switch as a key element of cytoplasmic maturation.