bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular spectrum of secretome regulates the relative hepatogenic potential of mesenchymal stem cells from bone marrow and dental tissue
Authors: Kumar Ajay , Kumar Vinod , Rattan Vidya , Jha Vivekananda , Pal Arnab , Bhattacharyya Shalmoli 
Published: November 2017

AbstractLiver regeneration is a spontaneous process that occurs after liver injury, but acute liver failure is a complex and fatal disease which is difficult to treat. Cell-based therapies are promising alternative therapeutic approach for liver failure and different cell sources have been tested in this regard. We investigated the comparative hepatogenic potential of human bone marrow stem cells (BMSC) with stem cells derived from human dental pulp (DPSC), apical papilla (SCAP) and follicle (DFSC) during this study. Hepatogenic potential of stem cells was assessed by functional assays at both genetic and protein level. We observed higher expression of most of the hepatic markers post differentiation in DPSCs compared to other cell types. LC-MS/MS analysis of stem cell secretome revealed the presence of different proteins related to hepatogenic lineage like growth arrest specific protein 6, oncostatin M, hepatocyte growth factor receptor etc. Interactome and Reactome pathway analysis revealed the interaction of DPSC/SCAP secretome proteins and these proteins were found to be associated with various pathways involved in lipid transport and metabolism. To the best of our knowledge, this is the first study regarding detailed investigation of hepatogenic potential of BMSCs v/s DMSCs (DPSC, SCAP & DFSC) along-with secretome characterization.
Quantitative text feature analysis of autobiographical interview data: prediction of episodic details, semantic details and temporal discounting
Authors: Peters J. , Wiehler A. , Bromberg U. 
Published: November 2017

AbstractAutobiographical memory and episodic future thinking (i.e. the capacity to project oneself into an imaginary future) are typically assessed using the Autobiographical Interview (AI). In the AI, subjects are provided with verbal cues (e.g. “your wedding day”) and are asked to freely recall (or imagine) the cued past (or future) event. Narratives are recorded, transcribed and analyzed using an established manual scoring procedure (Levine et al., 2002). Here we applied automatic text feature extraction methods to a relatively large (n = 86) set of AI data. In a first proof-of-concept approach, we used regression models to predict internal (episodic) and semantic detail sum scores from low-level linguistic features. Across a range of different regression methods, prediction accuracy averaged at about 0.5 standard deviations. Given the known association of episodic future thinking with temporal discounting behavior, i.e. the preference for smaller-sooner over larger-later rewards, we also ran models predicting temporal discounting directly from linguistic features of AI narratives. Here, prediction accuracy was much lower, but involved the same text feature components as prediction of internal (episodic) details. Our findings highlight the potential feasibility of using tools from quantitative text analysis to analyze AI datasets, and we discuss potential future applications of this approach.
Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
Authors: Franke Andrea , Roth Olivia , Schryver Peter De , Bayer Till , Garcia-Gonzalez Linsey , Künzel Sven , Bossier Peter , Miest Joanna J. , Clemmesen Catriona 
Published: November 2017

AbstractThe reliable production of marine fish larvae is one of the major bottlenecks in aquaculture due to high mortalities mainly caused by infectious diseases. To evaluate if the compound poly-β-hydroxybutyrate (PHB) might be a suitable immunoprophylactic measure in fish larviculture, its capacity to improve immunity and performance in European sea bass (Dicentrarchus labrax) yolk-sac larvae was explored. PHB was applied from mouth opening onwards to stimulate the developing larval immune system at the earliest possible point in time. Larval survival, growth, microbiota composition, gene expression profiles and disease resistance were assessed. PHB administration improved larval survival and, furthermore, altered the larva-associated microbiota composition. The bacterial challenge test using pathogenic Vibrio anguillarum revealed that the larval disease resistance was not influenced by PHB. The expression profiles of 26 genes involved e.g. in the immune response showed that PHB affected the expression of the antimicrobial peptides ferritin (fer) and dicentracin (dic), however, the response to PHB was inconsistent and weaker than previously demonstrated for sea bass post-larvae. Hence, the present study highlights the need for more research focusing on the immunostimulation of different early developmental stages for gaining a more comprehensive picture and advancing a sustainable production of high quality fry.
Extreme behavioural shifts by baboons exploiting risky, resource-rich, human-modified environments
Authors: Fehlmann Gaelle , O’Riain M. Justin , Kerr-Smith Catherine , Hailes Stephen , Luckman Adrian , Shepard Emily L. C. , King Andrew J. 
Published: November 2017

AbstractA range of species exploit anthropogenic food resources in behaviour known as ‘raiding’. Such behavioural flexibility is considered a central component of a species’ ability to cope with human-induced environmental changes. Here, we study the behavioural processes by which raiding male chacma baboons (Papio ursinus) exploit the opportunities and mitigate the risks presented by raiding in the suburbs of Cape Town, South Africa. Ecological sampling and interviews conducted with ‘rangers’ (employed to manage the baboons’ space use) revealed that baboons are at risk of being herded out of urban spaces that contain high-energy anthropogenic food sources. Baboon-attached motion/GPS tracking collars showed that raiding male baboons spent almost all of their time at the urban edge, engaging in short, high-activity forays into the urban space. Moreover, activity levels were increased where the likelihood of deterrence by rangers was greater. Overall, these raiding baboons display a time-activity balance that is drastically altered in comparison to individuals living in more remote regions. We suggest our methods can be used to obtain precise estimates of management impact for this and other species in conflict with people.
Decoy Wnt receptor (sLRP6E1E2)-expressing adenovirus induces anti-fibrotic effect via inhibition of Wnt and TGF-β signaling
Authors: Lee Won Jai , Lee Jung-Sun , Ahn Hyo Min , Na Youjin , Yang Chae Eun , Lee Ju Hee , Hong JinWoo , Yun Chae-Ok 
Published: November 2017

AbstractAberrant activation of the canonical Wingless type (Wnt) signaling pathway plays a key role in the development of hypertrophic scars and keloids, and this aberrant activation of Wnt pathway can be a potential target for the development of novel anti-fibrotic agents. In this study, we evaluated the anti-fibrotic potential of a soluble Wnt decoy receptor (sLRP6E1E2)-expressing non-replicating adenovirus (Ad; dE1-k35/sLRP6E1E2) on human dermal fibroblasts (HDFs), keloid fibroblasts (KFs), and keloid tissue explants. Higher Wnt3a and β-catenin expression was observed in the keloid region compared to the adjacent normal tissues. The activity of β-catenin and mRNA expression of type-I and -III collagen were significantly decreased following treatment with dE1-k35/sLRP6E1E2 in HDFs and KFs. The expression of LRP6, β-catenin, phosphorylated glycogen synthase kinase 3 beta, Smad 2/3 complex, and TGF-β1 were decreased in Wnt3a- or TGF-β1-activated HDFs, following administration of dE1-k35/sLRP6E1E2. Moreover, dE1-k35/sLRP6E1E2 markedly inhibited nuclear translocation of both β-catenin and Smad 2/3 complex. The expression levels of type-I and -III collagen, fibronectin, and elastin were also significantly reduced in keloid tissue explants after treatment with dE1-k35/sLRP6E1E2. These results indicate that Wnt decoy receptor-expressing Ad can degrade extracellular matrix in HDFs, KFs, and primary keloid tissue explants, and thus it may be beneficial for treatment of keloids.
Differential transcriptome regulation by 3,5-T2 and 3′,3,5-T3 in brain and liver uncovers novel roles for thyroid hormones in tilapia
Authors: Olvera A. , Martyniuk C. J. , Buisine N. , Jiménez-Jacinto V. , Sanchez-Flores A. , Sachs L. M. , Orozco A. 
Published: November 2017

AbstractAlthough 3,5,3′-triiodothyronine (T3) is considered to be the primary bioactive thyroid hormone (TH) due to its high affinity for TH nuclear receptors (TRs), new data suggest that 3,5-diiodothyronine (T2) can also regulate transcriptional networks. To determine the functional relevance of these bioactive THs, RNA-seq analysis was conducted in the cerebellum, thalamus-pituitary and liver of tilapia treated with equimolar doses of T2 or T3. We identified a total of 169, 154 and 2863 genes that were TH-responsive (FDR < 0.05) in the tilapia cerebellum, thalamus-pituitary and liver, respectively. Among these, 130, 96 and 349 genes were uniquely regulated by T3, whereas 22, 40 and 929 were exclusively regulated by T2 under our experimental paradigm. The expression profiles in response to TH treatment were tissue-specific, and the diversity of regulated genes also resulted in a variety of different pathways being affected by T2 and T3. T2 regulated gene networks associated with cell signalling and transcriptional pathways, while T3 regulated pathways related to cell signalling, the immune system, and lipid metabolism. Overall, the present work highlights the relevance of T2 as a key bioactive hormone, and reveals some of the different functional strategies that underpin TH pleiotropy.
Deconstructing atypical eye gaze perception in autism spectrum disorder
Authors: Pantelis Peter C. , Kennedy Daniel P. 
Published: November 2017

AbstractThe ability to discern the target of another person’s gaze is critical for social and linguistic development, but functions atypically in autism spectrum disorder (ASD). A multi-pronged approach allowed us to deconstruct this complex ability, to uncover the fundamental bases of this impairment. We analyzed performance on a novel gaze perception task with classical psychophysical metrics (precision and accuracy), principal component analysis (in the analysis of spatial biases), and Bayesian computational modeling (in the analysis of individual subjects’ use of contextual salience cues). Compared to controls, adults with ASD were less precise and less accurate in their judgments of gaze direction. Further, although nearly all controls exhibited a prototypical pattern of spatial bias in their judgments, this spatial prior was severely disrupted among a large subset of ASD participants. By contrast, Bayesian computational modeling revealed that both groups exploited contextual salience cues in their gaze judgments, and that the average strength of this contextual prior was similar for both groups. This comprehensive study revealed that although most ASD participants performed atypically in at least one aspect of gaze perception, the particular aspects disrupted varied idiosyncratically across individuals. Impairment in gaze perception in ASD likely arises via heterogeneous underlying mechanisms.
Mechanotransduction of matrix stiffness in regulation of focal adhesion size and number: reciprocal regulation of caveolin-1 and β1 integrin
Authors: Yeh Yi-Chun , Ling Jin-Ying , Chen Wan-Chun , Lin Hsi-Hui , Tang Ming-Jer 
Published: November 2017

AbstractFocal adhesion (FA) assembly, mediated by integrin activation, responds to matrix stiffness; however, the underlying mechanisms are unclear. Here, we showed that β1 integrin and caveolin-1 (Cav1) levels were decreased with declining matrix stiffness. Soft matrix selectively downregulated β1 integrin by endocytosis and subsequent lysosomal degradation. Disruption of lipid rafts with methyl-β-cyclodextrin or nystatin, or knockdown of Cav1 by siRNA decreased cell spreading, FA assembly, and β1 integrin protein levels in cells cultured on stiff matrix. Overexpression of Cav1, particularly the phospho-mimetic mutant Cav1-Y14D, averted soft matrix-induced decreases in β1 integrin protein levels, cell spreading, and FA assembly in NMuMG cells. Interestingly, overexpression of an auto-clustering β1 integrin hindered soft matrix-induced reduction of Cav1 and cell spreading, which suggests a reciprocal regulation between β1 integrin and Cav1. Finally, co-expression of this auto-clustering β1 integrin and Cav1-Y14D synergistically enhanced cell spreading, and FA assembly in HEK293T cells cultured on either stiff ( > G Pa) or soft (0.2 kPa) matrices. Collectively, these results suggest that matrix stiffness governs the expression of β1 integrin and Cav1, which reciprocally control each other, and subsequently determine FA assembly and turnover.
Impaired development of the cerebral cortex in infants with congenital heart disease is correlated to reduced cerebral oxygen delivery
Authors: Kelly Christopher J. , Makropoulos Antonios , Cordero-Grande Lucilio , Hutter Jana , Price Anthony , Hughes Emer , Murgasova Maria , Teixeira Rui Pedro A. G. , Steinweg Johannes K. , Kulkarni Sagar , Rahman Loay , Zhang Hui , Alexander Daniel C. , Pushparajah Kuberan , Rueckert Daniel , Hajnal Joseph V. , Simpson John , Edwards A. David , Rutherford Mary A. , Counsell Serena J. 
Published: November 2017

AbstractNeurodevelopmental impairment is the most common comorbidity associated with complex congenital heart disease (CHD), while the underlying biological mechanism remains unclear. We hypothesised that impaired cerebral oxygen delivery in infants with CHD is a cause of impaired cortical development, and predicted that cardiac lesions most associated with reduced cerebral oxygen delivery would demonstrate the greatest impairment of cortical development. We compared 30 newborns with complex CHD prior to surgery and 30 age-matched healthy controls using brain MRI. The cortex was assessed using high resolution, motion-corrected T2-weighted images in natural sleep, analysed using an automated pipeline. Cerebral oxygen delivery was calculated using phase contrast angiography and pre-ductal pulse oximetry, while regional cerebral oxygen saturation was estimated using near-infrared spectroscopy. We found that impaired cortical grey matter volume and gyrification index in newborns with complex CHD was linearly related to reduced cerebral oxygen delivery, and that cardiac lesions associated with the lowest cerebral oxygen delivery were associated with the greatest impairment of cortical development. These findings suggest that strategies to improve cerebral oxygen delivery may help reduce brain dysmaturation in newborns with CHD, and may be most relevant for children with CHD whose cardiac defects remain unrepaired for prolonged periods after birth.
A Thermodynamic Model of Diameter- and Temperature-dependent Semiconductor Nanowire Growth
Authors: Li Xinlei , Ni Jun , Zhang Ruiqin 
Published: November 2017

AbstractCreating and manipulating nanowires (NWs) with controllable growth direction and crystal orientation is important to meeting the urgent demands of emerging applications with designed properties. Revealing the underlying mechanisms of the experimentally demonstrated effects of NW diameter and growth temperature on growth direction is crucial for applications. Here, we establish a thermodynamic model to clarify the dependence of NW growth direction on diameter and temperature via the vapor-liquid-solid growth mechanism, enabling analysis of NW critical length between unstable and stable states. At a small critical length, NWs with a large diameter or grown at low temperature tend to grow along the <111> direction, while at a large critical length, NWs with a small diameter or grown at high temperature favor the <110> direction. Specific growth parameters of ZnSe NW have been obtained which can guide the design of functional NWs for applications.
Influence of hydrocarbon oil structure on adjuvanticity and autoimmunity
Authors: Yau Anthony C. Y. , Lönnblom Erik , Zhong Jianghong , Holmdahl Rikard 
Published: November 2017

AbstractMineral oils are extensively used in our daily life, in food, cosmetics, biomedicine, vaccines and in different industrial applications. However, exposure to these mineral oils has been associated with immune adjuvant effects and the development of autoimmune diseases. Here we investigate the structural impacts of the hydrocarbon oil molecules on their adjuvanticity and autoimmunity. First, we showed that hydrocarbon oil molecules with small atomic differences could result in experimental arthritis in DA rats differing in disease severity, incidence, weight change and serum levels of acute phase proteins. Injection of these hydrocarbon oils resulted in the activation, proliferation and elevated expression of Th1 and especially Th17 cytokines by the T cells, which correlate with the arthritogenicity of the T cells. Furthermore, the more arthritogenic hydrocarbon oils resulted in an increased production of autoantibodies against cartilage joint specific, triple-helical type II collagen epitopes. When injected together with ovalbumin, the more arthritogenic hydrocarbon oils resulted in an increased production of αβ T cell-dependent anti-ovalbumin antibodies. This study shows the arthritogenicity of hydrocarbon oils is associated with their adjuvant properties with implications to not only arthritis research but also other diseases and medical applications such as vaccines in which oil adjuvants are involved.
Gravitational Field effects on the Decoherence Process and the Quantum Speed Limit
Authors: Dehdashti Sh. , Avazzadeh Z. , Xu Z. , Shen J. Q. , Mirza B. , Wang H. 
Published: November 2017

AbstractIn this paper we use spinor transformations under local Lorentz transformations to investigate the curvature effect on the quantum-to-classical transition, described in terms of the decoherence process and of the quantum speed limit. We find that gravitational fields (introduced adopting the Schwarzschild and anti-de Sitter geometries) affect both the decoherence process and the quantum speed limit of a quantum particle with spin-1/2. In addition, as a tangible example, we study the effect of the Earth’s gravitational field, characterized by the Rindler space-time, on the same particle. We find that the effect of the Earth’s gravitational field on the decoherence process and quantum speed limit is very small, except when the mean speed of the quantum particle is comparable to the speed of light.
Behavioral changes after nicotine challenge are associated with α7 nicotinic acetylcholine receptor-stimulated glutamate release in the rat dorsal striatum
Authors: Ryu In Soo , Kim Jieun , Seo Su Yeon , Yang Ju Hwan , Oh Jeong Hwan , Lee Dong Kun , Cho Hyun-Wook , Yoon Seong Shoon , Seo Joung-Wook , Chang Suchan , Kim Hee Young , Shim Insop , Choe Eun Sang 
Published: November 2017

AbstractNeurochemical alterations associated with behavioral responses induced by re-exposure to nicotine have not been sufficiently characterized in the dorsal striatum. Herein, we report on changes in glutamate concentrations in the rat dorsal striatum associated with behavioral alterations after nicotine challenge. Nicotine challenge (0.4 mg/kg/day, subcutaneous) significantly increased extracellular glutamate concentrations up to the level observed with repeated nicotine administration. This increase occurred in parallel with an increase in behavioral changes in locomotor and rearing activities. In contrast, acute nicotine administration and nicotine withdrawal on days 1 and 6 did not alter glutamate levels or behavioral changes. Blockade of α7 nicotinic acetylcholine receptors (nAChRs) significantly decreased the nicotine challenge-induced increases in extracellular glutamate concentrations and locomotor and rearing activities. These findings suggest that behavioral changes in locomotor and rearing activities after re-exposure to nicotine are closely associated with hyperactivation of the glutamate response by stimulating α7 nAChRs in the rat dorsal striatum.
Influence of non-thermal plasma on structural and electrical properties of globular and nanostructured conductive polymer polypyrrole in water suspension
Authors: Galář Pavel , Khun Josef , Kopecký Dušan , Scholtz Vladimír , Trchová Miroslava , Fučíková Anna , Jirešová Jana , Fišer Ladislav 
Published: November 2017

AbstractNon-thermal plasma has proved its benefits in medicine, plasma assisted polymerization, food industry and many other fields. Even though, the ability of non-thermal plasma to modify surface properties of various materials is generally known, only limited attention has been given to exploitations of this treatment on conductive polymers. Here, we show study of non-thermal plasma treatment on properties of globular and nanostructured polypyrrole in the distilled water. We observe that plasma presence over the suspension level doesn’t change morphology of the polymer (shape), but significantly influences its elemental composition and physical properties. After 60 min of treatment, the relative concentration of chloride counter ions decreased approximately 3 and 4 times for nanostructured and globular form, respectively and concentration of oxygen increased approximately 3 times for both forms. Simultaneously, conductivity decrease (14 times for globular and 2 times for nanostructured one) and changes in zeta potential characteristics of both samples were observed. The modification evolution was dominated by multi-exponential function with time constants having values approximately 1 and 10 min for both samples. It is expected that these time constants are related to two modification processes connected to direct presence of the spark and to long-lived species generated by the plasma.
Gut remediation: a potential approach to reducing chromium accumulation using Lactobacillus plantarum TW1-1
Authors: Wu Gaofeng , Xiao Xingpeng , Feng Pengya , Xie Fuquan , Yu Zhengsheng , Yuan Wenzhen , Liu Pu , Li Xiangkai 
Published: November 2017

AbstractSome lactobacilli have protective effects against some heavy metals in mammals, but the underlying mechanism is not fully understood. To evaluate the remediation potency and the mechanism of Lactobacillus against chromium (Cr) in mice, Lactobacillus plantarum TW1-1 was orally administrated to Kunming mice for 7 weeks during exposure to 1 mM K2Cr2O7 in drinking water. Results showed that TW1-1 helped to decrease Cr accumulation in tissues and increase Cr excretion in feces, and may also attenuate alterations in oxidative stress and histopathological changes caused by Cr exposure. Moreover, the chromate reduction ability of fecal bacteria doubled after administration of TW1-1 upon Cr induction. MiSeq sequencing of fecal bacterial 16S rRNA genes revealed that the overall structures of gut microbiota was shifted by Cr exposure and partially restored by TW1-1. The abundances of 49 of the 79 operational taxonomic units altered by Cr were reversed by TW1-1. Based on these, we proposed a working model of TW1-1 against Cr: TW1-1 helps to remove Cr from the host and meanwhile acts as a regulator of gut microbiota, which aids in chromate reduction and provide protection against Cr. We call this process of remediation of heavy metal in the gut “gut remediation”.