bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of Lampenflora Chlorobium limicola Strain Frasassi in a Sulfidic Cave System
Authors: Mansor Muammar , Macalady Jennifer L. 
Published: May 2016

ABSTRACT

The draft genome sequence of
Chlorobium limicola
strain Frasassi was assembled from metagenomic sequencing of a green mat in an artificially lighted aquarium inside the Frasassi caves in Italy. The genome is 2.08 Mbp in size and contains the necessary genes for anoxygenic photosynthesis and CO
2
fixation.
Draft Genome Sequences of Four Plant Probiotic
Bacillus
Strains
Authors: Jeong Haeyoung , Park Seung-Hwan , Choi Soo-Keun 
Published: May 2016

ABSTRACT

Here, we report the whole-genome sequences of four
Bacillus
strains that exhibit plant probiotic activities. Three of them are the type strains of
Bacillus endophyticus
, “
Bacillus gaemokensis
,” and
Bacillus trypoxylicola
, and the other,
Bacillus
sp. strain KCTC 13219, should be reclassified into a species belonging to the genus
Lysinibacillus
.
Complete Genome Sequences of Zika Virus Strains Isolated from the Blood of Patients in Thailand in 2014 and the Philippines in 2012
Authors: Ellison D. W. , Ladner J. T. , Buathong R. , Alera M. T. , Wiley M. R. , Hermann L. , Rutvisuttinunt W. , Klungthong C. , Chinnawirotpisan P. , Manasatienkij W. , Melendrez M. C. , Maljkovic Berry I. , Thaisomboonsuk B. , Ong-ajchaowlerd P. , Kaneechit W. , Velasco J. M. , Tac-An I. A. , Villa D. , Lago C. B. , Roque V. G. , Plipat T. , Nisalak A. , Srikiatkhachorn A. , Fernandez S. , Yoon I. K. , Haddow A. D. , Palacios G. F. , Jarman R. G. , Macareo L. R. 
Published: May 2016

ABSTRACT

Here, we present the complete genome sequences of two Zika virus (ZIKV) strains, Zika virus/
Homo sapiens
-tc/THA/2014/SV0127-14 and Zika virus/
H. sapiens
-tc/PHL/2012/CPC-0740, isolated from the blood of patients collected in Thailand, 2014, and the Philippines, 2012, respectively. Sequencing and phylogenetic analysis showed that both strains belong to the Asian lineage.
Draft Genome Sequence of
Cutaneotrichosporon curvatus
DSM 101032 (Formerly
Cryptococcus curvatus
), an Oleaginous Yeast Producing Polyunsaturated Fatty Acids
Authors: Hofmeyer Thomas , Hackenschmidt Silke , Nadler Florian , Thürmer Andrea , Daniel Rolf , Kabisch Johannes 
Published: May 2016

ABSTRACT

Cutaneotrichosporon curvatus
DSM 101032 is an oleaginous yeast that can be isolated from various habitats and is capable of producing substantial amounts of polyunsaturated fatty acids. Here, we present the first draft genome sequence of any
C. curvatus
species.
Draft Genome Sequence of Hydrocarbon-Degrading Staphylococcus saprophyticus Strain CNV2, Isolated from Crude Oil-Contaminated Soil from the Noonmati Oil Refinery, Guwahati, Assam, India
Authors: Mukherjee Arghya , Chettri Bobby , Langpoklakpam James S. , Singh Arvind K. , Chattopadhyay Dhrubajyoti 
Published: May 2016

ABSTRACT

Here, we report the 2.6 Mb draft genome sequence of hydrocarbon-degrading
Staphylococcus saprophyticus
strain CNV2, isolated from oil-contaminated soil in Guwahati, India. CNV2 contains 2,545 coding sequences and has a G+C content of 33.2%. This is the first report of the genome sequence of an
S. saprophyticus
adapted to an oil-contaminated environment.
Complete Genome Sequence of Staphylococcus aureus MCRF184, a Necrotizing Fasciitis-Causing Methicillin-Sensitive Sequence Type 45
Staphylococcus
Strain
Authors: Aswani Vijay , Mau Bob , Shukla Sanjay K. 
Published: May 2016

ABSTRACT

We report here the complete genome sequence of a highly virulent methicillin-sensitive
Staphylococcus aureus
strain
,
MCRF184, belonging to sequence type 45. This staphylococcal strain was isolated from a surgical biopsy specimen from a patient with necrotizing fasciitis.
Complete Genome Sequences of Five Zika Virus Isolates
Authors: Ladner Jason T. , Wiley Michael R. , Prieto Karla , Yasuda Chadwick Y. , Nagle Elyse , Kasper Matthew R. , Reyes Daniel , Vasilakis Nikolaos , Heang Vireak , Weaver Scott C. , Haddow Andrew , Tesh Robert B. , Sovann Ly , Palacios Gustavo 
Published: May 2016

ABSTRACT
Zika virus is an emerging human pathogen of great concern due to putative links to microcephaly and Guillain-Barre syndrome. Here, we report the complete genomes, including the 5′ and 3′ untranslated regions, of five Zika virus isolates, one from the Asian lineage and four from the African lineage.
First Insights into the Genome of the Moderately Thermophilic Bacterium Clostridium tepidiprofundi SG 508
T
Authors: Poehlein Anja , Friedrich Ines , Krüger Larissa , Daniel Rolf 
Published: May 2016

ABSTRACT

The moderately thermophilic bacterium
Clostridium tepidiprofundi
is Gram-positive and belongs to clostridial cluster I. It was isolated from a hydrothermal vent chimney. Substrates utilized by
C. tepidiprofundi
include casein, peptone, tryptone, yeast extract, beef extract, starch, maltose, and glucose. The genome consists of one replicon (3.06 Mb).
Seroprevalence and correlates ofToxoplasma gondiiinfection in Yoremes (Mayos) in Mexico: a cross-sectional study
Authors: Alvarado-Esquivel Cosme , Rascón-Careaga Antonio , Hernández-Tinoco Jesús , Corella-Madueño María Alba Guadalupe , Sánchez-Anguiano Luis Francisco , Aldana-Madrid María Lourdes , Almada-Balderrama Gerardo Javier , Nuñez-Aguirre Alan Daniel , Liesenfeld Oliver 
Published: May 2016

ObjectivesWe sought to determine the prevalence of anti-Toxoplasma gondiiantibodies in Yoremes and to identify associations ofT. gondiiexposure with sociodemographic, clinical and behavioural characteristics of Yoremes.DesignA cross-sectional survey.SettingYoremes were enrolled in the locality of Tierra Blanca in the municipality of Navojoa in Sonora State, Mexico.ParticipantsWe studied 200 Yoremes (Mayos); they are an indigenous ethnic group living in a coastal region in northwestern Mexico.Primary and secondary outcome measuresWe assessed the prevalence of anti-ToxoplasmaIgG and IgM antibodies in participants using enzyme-linked immunoassays. We used a standardised questionnaire to obtain the characteristics of Yoremes. The association ofT. gondiiexposure and Yoremes’ characteristics was assessed by bivariate and multivariate analyses.ResultsOf the 200 Yoremes studied (mean age: 31.50±18.43 years), 26 (13.0%) were positive for anti-T. gondiiIgG antibodies and 19 (73.1%) of them were also positive for anti-T. gondiiIgM antibodies. Seroprevalence ofT. gondiiinfection did not vary with sex, educational level, occupation or socioeconomic status. In contrast, multivariate analysis of sociodemographic and behavioural characteristics showed thatT. gondiiexposure was associated with increasing age (OR=1.02; 95% CI 1.00 to 1.04; p=0.03) and consumption of squirrel meat (OR=4.99; 95% CI 1.07 to 23.31; p=0.04). Furthermore, seroprevalence ofT. gondiiinfection was significantly higher in Yoremes with a history of lymphadenopathy (p=0.03) and those suffering from frequent abdominal pain (p=0.03). In women,T. gondiiexposure was associated with a history of caesarean sections (p=0.03) and miscarriages (p=0.02).ConclusionsWe demonstrate, for the first time, serological evidence ofT. gondiiexposure among Yoremes in Mexico. Results suggest that infection withT. gondiimight be affecting the health of Yoremes. Results may be useful for an optimal design of preventive measures againstT. gondiiinfection.
Association between fluid intake and kidney function, and survival outcomes analysis: a nationwide population-based study
Authors: Wu Li-Wei , Chen Wei-Liang , Liaw Fang-Yih , Sun Yu-Shan , Yang Hui-Fang , Wang Chung-Ching , Lin Chien-Ming , Tsao Yu-Tzu 
Published: May 2016

ObjectivesFluid intake, one of the most common daily activities, has not been well studied in chronic kidney disease (CKD) populations, and clinical outcomes are rarely addressed. The aim of this nationwide study is to explore the influence of daily fluid intake on cardiovascular and all-cause mortality and its association with renal function.DesignObservational cohort study.ParticipantsIn all, 2182 participants aged more than 20 years participated in the Third National Health and Nutrition Examination Survey (1988–1994).Main outcome measuresSurvival outcomes in patients with or without CKD, using multiple variable adjusted Cox proportional hazard models.ResultsIn a longitudinal survey with a median follow-up length of 15.4 years, 1080 participants died and 473 cardiovascular deaths were recorded. For all-cause mortality in the CKD group, individuals in the highest quartile of fluid intake (≧3.576 L/day) had better survival outcomes than those in the lowest quartile of fluid intake (≤2.147 L/day) (p=0.029) after adjustment of several pertinent variables.ConclusionsAlthough the interpretation of this observational study was limited by the failure to identify the compositions of ingested fluids, adequate hydration may offer some advantages in patients with CKD. However, the underlying pathophysiological mechanisms of the responses of normal and injured kidneys to chronic changes in fluid consumption warrant further investigation.
Layperson mouth-to-mask ventilation using a modified I-gel laryngeal mask after brief onsite instruction: a manikin-based feasibility trial
Authors: Schälte Gereon , Bomhard Lilli-Theresa , Rossaint Rolf , Coburn Mark , Stoppe Christian , Zoremba Norbert , Rieg Annette 
Published: May 2016

ObjectiveThe intention of this manikin-based trial was to evaluate whether laypersons are able to operate an I-gel laryngeal mask (I-gel) modified for mouth-to-mask ventilation after receiving brief on-site instruction.SettingEntrance hall of a university hospital and the city campus of a public technical university, using a protected manikin scenario.MethodsLaypersons were handed a labelled, mouthpiece-integrated I-gel laryngeal mask and a corresponding instruction chart and were asked to follow the printed instructions.Outcome measuresThe overall process was analysed and evaluated according to quality and duration.ResultsData from 100 participants were analysed. Overall, 79% of participants were able to effectively ventilate the manikin, 90% placed the laryngeal mask with the correct turn and direction, 19% did not position the mask deep enough and 85% believed that their inhibition threshold for performing resuscitation was lowered. A significant reduction in reluctance before and after the trial was found (p<0.0001). A total of 35% of participants had concerns about applying first aid in an emergency. Former basic life support (BLS) training significantly reduced the time of insertion (19.6 s, 95% CI 17.8 to 21.5, p=0.0004) and increased overall success (p=0.0096).ConclusionsLaypersons were able to manage mouth-to-mask ventilation in the manikin with a reasonable success rate after receiving brief chart-based on-site instructions using a labelled I-gel mask. Positioning the mask deep enough and identifying whether the manikin was successfully ventilated were the main problems observed. A significant reduction in reluctance towards initialising BLS by using a modified supraglottic airway device (SAD) may lead to better acceptance of bystander resuscitation in laypersons, supporting the introduction of SADs into BLS courses and the stocking of SADs in units with public automatic external defibrillators.
Out-of-hours antibiotic prescription after screening with C reactive protein: a randomised controlled study
Authors: Rebnord Ingrid Keilegavlen , Sandvik Hogne , Batman Mjelle Anders , Hunskaar Steinar 
Published: May 2016

ObjectiveTo evaluate the effect of preconsultation C reactive protein (CRP) screening on antibiotic prescribing and referral to hospital in Norwegian primary care settings with low prevalence of serious infections.DesignRandomised controlled observational study at out-of-hours services in Norway.SettingPrimary care.Participants401 children (0–6 years) with fever and/or respiratory symptoms were recruited from 5 different out-of-hours services (including 1 paediatric emergency clinic) in 2013–2015.InterventionData were collected from questionnaires and clinical examination results. Every third child was randomised to a CRP test before the consultation; for the rest, the doctor ordered a CRP test if considered necessary.Outcome measuresMain outcome variables were prescription of antibiotics and referral to hospital.ResultsIn the group pretested with CRP, the antibiotic prescription rate was 26%, compared with 22% in the control group. In the group pretested with CRP, 5% were admitted to hospital, compared with 9% in the control group. These differences were not statistically significant. The main predictors for ordering a CRP test were parents' assessment of seriousness of the illness and the child's temperature. Paediatricians ordered CRP tests less frequently than did other doctors (9% vs 56%, p<0.001).ConclusionsPreconsultation screening with CRP of children presenting to out-of-hours services with fever and/or respiratory symptoms does not significantly affect the prescription of antibiotics or referral to hospital.Trial registration numberNCT02496559; Results.
First-principles study of optical properties of InN nanosheet
Authors: Sarmazdeh Masoud Majidiyan , Mendi Roohallah Taghavi , Zelati Amir , Boochani Arash , Nofeli Fariba 
Published: May 2016

Based on density functional theory (DFT), some optical properties of InN nanosheet, such as dielectric function, energy loss function, refractive index, reflectivity and absorption coefficient, have been calculated using the modified Becke–Johnson (mBJ) exchange–correlation potential and full potential-linearized augmented plane waves (FP-LAPW) method. The study of dielectric function show that optical properties of InN nanosheet are anisotropic and important energy range in the optical process is between low energies to 20 eV. The results indicate the plasmon energy of InN nanosheet occurs in the lower energy than bulk InN and in addition the plasmon energy in the in-plane direction is different from that perpendicular to the in-plane direction. The obtained optical gaps are 1.2 eV and 3.6 eV in perpendicular and parallel to [Formula: see text]-axis, respectively. Study of refractive index and optical reflectivity shows that the superluminal phenomena occur in the several energy ranges for the InN nanosheet and this nanosheet has high transparency in a wide energy range. The results propose that the InN nanosheet is a good candidate for the optical communications applications, optoelectronics devices and transparent coatings.
Quantum turing machine and brain model represented by Fock space
Authors: Iriyama Satoshi , Ohya Masanori 
Published: May 2016

The adaptive dynamics is known as a new mathematics to treat with a complex phenomena, for example, chaos, quantum algorithm and psychological phenomena. In this paper, we briefly review the notion of the adaptive dynamics, and explain the definition of the generalized Turing machine (GTM) and recognition process represented by the Fock space. Moreover, we show that there exists the quantum channel which is described by the GKSL master equation to achieve the Chaos Amplifier used in [M. Ohya and I. V. Volovich, J. Opt. B 5(6) (2003) 639., M. Ohya and I. V. Volovich, Rep. Math. Phys. 52(1) (2003) 25.]
Concomitant Inhibition of PI3Kβ and BRAF or MEK in PTEN-Deficient/BRAF-Mutant Melanoma Treatment: Preclinical Assessment of SAR260301 Oral PI3Kβ-Selective Inhibitor
Authors: Bonnevaux Hélène , Lemaitre Olivier , Vincent Loic , Levit Mikhail N. , Windenberger Fanny , Halley Frank , Delorme Cécile , Lengauer Christoph , Garcia-Echeverria Carlos , Virone-Oddos Angela 
Published: May 2016

Abstract
Class IA PI3K pathway activation resulting from PTEN deficiency has been associated with lack of sensitivity of melanoma to BRAF kinase inhibitors. Although previous studies have shown synergistic activity when pan-PI3K inhibitors were combined with MAPK inhibitors in the treatment of melanoma exhibiting concurrent genetic abnormalities, overlapping adverse events in patients limit optimal dosing and clinical application. With the aim of specifically targeting PTEN-deficient cancers and minimizing the potential for on-target toxicity when inhibiting multiple PI3K isoforms, we developed a program to discover PI3Kβ-selective kinase inhibitors and identified SAR260301 as a potent PI3Kβ-selective, orally available compound, which is now in clinical development. Herein, we provide a detailed biological characterization of SAR260301, and show that this compound has outstanding biochemical and cellular selectivity for the PI3Kβ isoform versus the α, δ, and γ isoforms and a large panel of protein and lipid kinases. We demonstrate that SAR260301 blocks PI3K pathway signaling preferentially in PTEN-deficient human tumor models, and has synergistic antitumor activity when combined with vemurafenib (BRAF inhibitor) or selumetinib (MEK inhibitor) in PTEN-deficient/BRAF-mutated human melanoma tumor models. Combination treatments were very well tolerated, suggesting the potential for a superior safety profile at optimal dosing using selective compounds to inhibit multiple signaling pathways. Together, these experiments provide a preclinical proof-of-concept for safely combining inhibitors of PI3Kβ and BRAF or MEK kinase modulators to improve antitumor activity in PTEN-deficient/BRAF-mutant melanoma, and support the evaluation of SAR260301-based combinations in clinical studies. Mol Cancer Ther; 15(7); 1460–71. ©2016 AACR.
Hepatic lipase inactivation decreases atherosclerosis in insulin resistance by reducing LIGHT/Lymphotoxin β-Receptor pathway
Authors: Andrés-Blasco Irene , Vinué Àngela , Herrero-Cervera Andrea , Martínez-Hervás Sergio , Nuñez Laura , Piqueras Laura , Ascaso Juan F. , Sanz MarÍa Jesús , Burks Deborah Jane , González-Navarro Herminia 
Published: May 2016

SummaryCoexistence of insulin resistance (IR) and metabolic syndrome (MetS) increases the risk of cardiovascular disease (CVD). Genetic studies in diabetes have linked Hepatic Lipase (HL) to an enhanced risk of CVD while others indicate a role of HL in inflammatory cells. Thus, we explored the role of HL on atherosclerosis and inflammation in a mouse model of MetS/IR, (apoE-/-Irs2+/- mice) and in patients with MetS and IR. HL-deficiency in apoE-/-Irs2+/- mice reduced atheroma size, plaque vulnerability, leukocyte infiltration and macrophage proliferation. Compared with apoE-/-Irs2+/-HL+/+ mice, MCP1, TNFa and IL6 plasma levels, pro-inflammatory Ly6Chi monocytes and activated(CD69+)-T lymphocytes were also decreased in apoE-/-Irs2+/-HL-/- mice. The LIGHT (Tumour necrosis factor ligand superfamily member 14, TNFSF14)/ Lymphotoxin β-Receptor(LTβ-R) pathway, which is involved in T-cell and macrophage activation, was diminished in plasma and in apoE-/-Irs2+/-HL-/- mouse atheromas. Treatment of apoE-/-Irs2+/-HL-/- mice with LIGHT increased the number of Ly6Chi-monocytes and lesion size. Acutely LIGHT-treated apoE-/- mice displayed enhanced proliferating Ly6Chi-monocytes and increased activation of the mitogen-activated protein kinase p38, suggesting that LIGHT/LTβ-R axis might promote atherogenesis by increasing proinflammatory monocytes and proliferation. Notably, MetS-IR subjects with increased atherosclerosis displayed up-regulation of the LIGHT/LTβ-R axis, enhanced inflammatory monocytes and augmented HL mRNA expression in circulating leukocytes. Thus, HL-deficiency decreases atherosclerosis in MetS/IR states by reducing inflammation and macrophage proliferation which are partly attributed to reduced LIGHT/LTβ-R pathway. These studies identify the LIGHT/LTβ-R axis as a main pathway in atherosclerosis and suggest that its inactivation might ameliorate inflammation and macrophage proliferation associated with atherosclerosis burden in MetS/IR.Supplementary Material to this article is available at www.thrombosis-online.com.
Selective Sphingosine-1-Phosphate Receptor 1 Modulation Attenuates Experimental Intracerebral Hemorrhage
Authors: Sun Na , Shen Yi , Han Wei , Shi Kaibin , Wood Kristofer , Fu Ying , Hao Junwei , Liu Qiang , Sheth Kevin N. , Huang DeRen , Shi Fu-Dong 
Published: May 2016

Background and Purpose—
Preclinical studies and a proof-of-concept clinical study have shown that sphingosine-1-phosphate receptor (S1PR) modulator, fingolimod, improves the clinical outcome of intracerebral hemorrhage (ICH). However, the specific subtype of the S1PRs through which immune modulation provides protection in ICH remains unclear. In addition, fingolimod-induced adverse effects could limit its use in patients with stroke because of interactions with other S1PR subtypes, particularly with S1PR3. RP101075 is a selective S1PR1 agonist with superior cardiovascular safety profile. In this study, we investigated the impact of RP101075 treatment in a mouse model of ICH.

Methods—

ICH was induced by injection of autologous blood in 294 male C57BL/6J and Rag2
−/−
mice. ICH mice randomly received vehicle, RP101075, or RP101075 plus S1PR1 antagonist W146 by daily oral gavage for three consecutive days, starting from 30 minutes after surgery. Neurodeficits, brain edema, brain infiltration of immune cells, blood–brain barrier integrity, and cell death were assessed after ICH.

Results—
RP101075 significantly attenuated neurological deficits and reduced brain edema in ICH mice. W146 blocked the effects of RP101075 on neurodeficits and brain edema. RP101075 reduced the counts of brain-infiltrating lymphocytes, neutrophils, and microglia, as well as cytokine expression after ICH. Enhanced blood–brain barrier integrity and alleviated neuronal death were also seen in ICH mice after RP101075 treatment.

Conclusions—
S1PR1 modulation via RP101075 provides protection in experimental ICH. Together with the advantageous pharmacological features of RP101075, these results warrant further investigations of its mechanisms of action and translational values in ICH patients.
External Validation of the ASTRAL and DRAGON Scores for Prediction of Functional Outcome in Stroke
Authors: Cooray Charith , Mazya Michael , Bottai Matteo , Dorado Laura , Skoda Ondrej , Toni Danilo , Ford Gary A. , Wahlgren Nils , Ahmed Niaz 
Published: May 2016

Background and Purpose—
ASTRAL (Acute Stroke Registry and Analysis of Lausanne) and DRAGON (includes dense middle cerebral artery sign, prestroke modified Rankin Scale score, age, glucose, onset to treatment, National Institutes of Health Stroke Scale score) are 2 recently developed scores for predicting functional outcome after acute stroke in unselected acute ischemic stroke patients and in patients treated with intravenous thrombolysis, respectively. We aimed to perform external validation of these scores to assess their predictive performance in the large multicentre Safe Implementation of Thrombolysis in Stroke-International Stroke Thrombolysis Register.

Methods—
We calculated the ASTRAL and DRAGON scores in 36 131 and 33 716 patients, respectively, registered in Safe Implementation of Thrombolysis in Stroke-International Stroke Thrombolysis Register between 2003 and 2013. The proportion of patients with 3-month modified Rankin Scale scores of 3 to 6 was observed for each score point and compared with the predicted proportion according to the risk scores. Calibration was assessed using calibration plots, and predictive performance was assessed using area under the curve of the receiver operating characteristic. Multivariate logistic regression coefficients for the variables in the 2 scores were compared with the original derivation cohorts.

Results—
The ASTRAL showed an area under the curve of 0.790 (95% confidence interval, 0.786–0.795) and the DRAGON an area under the curve of 0.774 (95% confidence interval, 0.769–0.779). All ASTRAL parameters except range of visual fields and all DRAGON parameters were significantly associated with functional outcome in multivariate analysis.

Conclusions—
The ASTRAL and DRAGON scores show an acceptable predictive performance. ASTRAL does not require imaging-data and therefore may have an advantage for the use in prehospital patient assessment. Prospective studies of both scores evaluating the impact of their use on patient outcomes after intravenous thrombolysis and endovascular therapy are needed.