bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceptions of French and Spanish Slave Law in Late Eighteenth-Century Britain
Authors: Wyman-McCarthy Matthew 
Published: January 2018

AbstractThis article examines British understandings of the laws and legal traditions that regulated slavery in French and Spanish colonies in the late eighteenth century, particularly between the American and French Revolutions. Based on reports from those with firsthand knowledge of different slave systems, many imperial commentators contended that enslaved persons under French and Spanish rule were treated more humanely—and consequently worked more efficiently—than those in British jurisdictions. Advocates of slavery reform therefore looked to the slave management strategies of competitors to help advance their cause. For some, appropriating foreign slave regulations became a central feature of programs designed to lessen the brutality of slavery and eventually bring about emancipation. For others, highlighting the comparatively benign treatment of enslaved workers in French and Spanish islands served as a way to pressure the British government to more proactively police slaveholding in its own colonies. By exploring calls to emulate the slave regulations of rival empires, this article provides a window onto shifting British attitudes toward both slavery and imperial governance during a period of major political and economic change in the Atlantic World.
Crime Scene Photography in England, 1895–1960
Authors: Bell Amy 
Published: January 2018

AbstractThis article discusses the development of techniques and practices of murder crime scene photography through four pairs of photographs taken in England between 1904 and 1958 and examines their “forensic aesthetic”: the visual combination of objective clues and of subjective aesthetic resonances. Crime scene photographs had legal status as evidence that had to be substantiated by a witness, and their purpose, as expressed in forensic textbooks and policing articles, was to provide a direct transfer of facts to the courtroom; yet their inferential visual nature made them allusive and evocative as well. Each of four pairs of photographs discussed reflects a significant period in the historical evolution of crime scene photography as well as an observable aesthetic influence: the earliest days of police photography and pictorialism; professionalization in the 1930s, documentary photography, and film noir; postwar photographic expansion to the suburban and middle class, advertising images of the family and home; and postwar elegiac landscape photography in the 1950s and compassion shown to infanticidal mothers. Crime scene photographs also demonstrate a remarkable shift in twentieth-century forensic technologies, and they reveal a collection of ordinary domestic and pastoral scenes at the moment when an act of violence made them extraordinary.
LABOUR PROVISIONS IN EU AND US MEGA-REGIONAL TRADE AGREEMENTS: RHETORIC AND REALITY
Authors: Melo Araujo Billy 
Published: January 2018

AbstractThe EU and the US have long called for the linking of trade and labour standards in trade agreements at both the multilateral and bilateral level. This article examines their practice of including labour provisions in trade agreements, with a particular focus on recent attempts to include such provisions on so-called ‘mega-regionals’, which were presented by their proponents as providing the benchmark for labour protection in future trade agreements. It discusses the rationale behind the inclusion of such provisions and their practical limitations, and examines the extent to which mega-regionals address these limitations. It is argued that whilst the EU and the US have been keen advocates for trade-labour linkages, there has also been an unwillingness to convert this rhetoric into practice, raising questions about the extent of their commitment to these values.
UNDERSTANDING AND OVERCOMING CHALLENGES IN ACCESSING THE AFRICAN COURT ON HUMAN AND PEOPLES’ RIGHTS
Authors: Viljoen Frans 
Published: January 2018

AbstractThis contribution examines access to the African Court on Human and Peoples’ Rights in the first decade of its operation. Compared with other regional human rights Courts over the corresponding period, the African Court has decided more contentious cases. Direct access accounts for this difference. Acceptance by States of optional direct access is a necessary but insufficient condition for actual access. The reasons for the Commission's reluctance to refer cases, which hampered indirect access to the Court, are investigated. Although the Court's advisory jurisdiction has found limited application, it has welcomed amici curiae and showed some acceptance of the role of original complainants before the Court.
JUDICIAL COOPERATION IN CIVIL AND COMMERCIAL MATTERS AFTER BREXIT: WHICH WAY FORWARD?
Authors: Rühl Giesela 
Published: January 2018

AbstractJudicial cooperation in civil and commercial matters is generally perceived to be of a rather ‘specialist and technical nature’. However, for the many UK and EU citizens, families and businesses who work, live, travel and do business abroad, the current European framework for choice of law, jurisdiction and recognition and enforcement is of paramount importance. The article, therefore, explores how that framework might look like after Brexit and explores the merits and demerits of the various ways forward. It argues that the best option for both the UK and the EU would be to agree on the continued application of the existing EU instruments or to strive for conclusion of a new agreement that closely replicates these instruments. If no such agreement can be reached the UK should decide to apply the Rome I and Rome II Regulations unilaterally and sign the Lugano Convention of 2007 as well as the Hague Convention on Choice of Court Agreements of 2005.
From accuracy to accountability: subjecting global indicators to the rule of law
Authors: Frydman Benoit 
Published: January 2018

AbstractThis paper presents a double genealogy of indicators as instruments of governance. These have their roots both in the use of statistical tools for normative purposes by states and in the development of indicators within firms as preferred instruments of ‘new management’. The paper argues that social indicators not only convey information, but are genuine tools of global governance and that, for this reason, their legitimacy depends not only on their accuracy, but also on their accountability. If indicators are intended to produce or effectively produce regulatory effects, they should be subjected to the rule of law and to judicial review. The essay ends by formulating four main principles that these indicators should comply with.
Oxidative stress preconditioning of mouse perivascular myogenic progenitors selects a subpopulation of cells with a distinct survival advantage in vitro and in vivo
Authors: Gargioli Cesare , Turturici Giuseppina , Barreca Maria M. , Spinello Walter , Fuoco Claudia , Testa Stefano , Feo Salvatore , Cannata Stefano M. , Cossu Giulio , Sconzo Gabriella , Geraci Fabiana 
Published: January 2018

AbstractCell engraftment, survival and integration during transplantation procedures represent the crux of cell-based therapies. Thus, there have been many studies focused on improving cell viability upon implantation. We used severe oxidative stress to select for a mouse mesoangioblast subpopulation in vitro and found that this subpopulation retained self-renewal and myogenic differentiation capacities while notably enhancing cell survival, proliferation and migration relative to unselected cells. Additionally, this subpopulation of cells presented different resistance and recovery properties upon oxidative stress treatment, demonstrating select advantages over parental mesoangioblasts in our experimental analysis. Specifically, the cells were resistant to oxidative environments, demonstrating survival, continuous self-renewal and improved migration capability. The primary outcome of the selected cells was determined in in vivo experiments in which immunocompromised dystrophic mice were injected intramuscularly in the tibialis anterior with selected or non-selected mesoangioblasts. Resistant mesoangioblasts exhibited markedly enhanced survival and integration into the host skeletal muscle, accounting for a more than 70% increase in engraftment compared with that of the unselected mesoangioblast cell population and leading to remarkable muscle recovery. Thus, the positive effects of sorting on mesoangioblast cell behaviour in vitro and in vivo suggest that a selection step involving oxidative stress preconditioning may provide a novel methodology to select for resistant cells for use in regenerative tissue applications to prevent high mortality rates upon transplantation.
Experimental noise cutoff boosts inferability of transcriptional networks in large-scale gene-deletion studies
Authors: Blum C. F. , Heramvand N. , Khonsari A. S. , Kollmann M. 
Published: January 2018

AbstractGenerating a comprehensive map of molecular interactions in living cells is difficult and great efforts are undertaken to infer molecular interactions from large-scale perturbation experiments. Here, we develop the analytical and numerical tools to quantify the fundamental limits for inferring transcriptional networks from gene knockout screens and introduce a network inference method that is unbiased with respect to measurement noise and scalable to large network sizes. We show that network asymmetry, knockout coverage and measurement noise are central determinants that limit prediction accuracy, whereas the knowledge about gene-specific variability among biological replicates can be used to eliminate noise-sensitive nodes and thereby boost the performance of network inference algorithms.
Biotransformation of polyunsaturated fatty acids to bioactive hepoxilins and trioxilins by microbial enzymes
Authors: An Jung-Ung , Song Yong-Seok , Kim Kyoung-Rok , Ko Yoon-Joo , Yoon Do-Young , Oh Deok-Kun 
Published: January 2018

AbstractHepoxilins (HXs) and trioxilins (TrXs) are involved in physiological processes such as inflammation, insulin secretion and pain perception in human. They are metabolites of polyunsaturated fatty acids (PUFAs), including arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid, formed by 12-lipoxygenase (LOX) and epoxide hydrolase (EH) expressed by mammalian cells. Here, we identify ten types of HXs and TrXs, produced by the prokaryote Myxococcus xanthus, of which six types are new, namely, HXB5, HXD3, HXE3, TrXB5, TrXD3 and TrXE3. We succeed in the biotransformation of PUFAs into eight types of HXs (>35% conversion) and TrXs (>10% conversion) by expressing M. xanthus 12-LOX or 11-LOX with or without EH in Escherichia coli. We determine 11-hydroxy-eicosatetraenoic acid, HXB3, HXB4, HXD3, TrXB3 and TrXD3 as potential peroxisome proliferator-activated receptor-γ partial agonists. These findings may facilitate physiological studies and drug development based on lipid mediators.
Pushing nuclear magnetic resonance sensitivity limits with microfluidics and photo-chemically induced dynamic nuclear polarization
Authors: Mompeán Miguel , Sánchez-Donoso Rosa M. , de la Hoz Antonio , Saggiomo Vittorio , Velders Aldrik H. , Gomez M. Victoria 
Published: January 2018

AbstractAmong the methods to enhance the sensitivity of nuclear magnetic resonance (NMR) spectroscopy, small-diameter NMR coils (microcoils) are promising tools to tackle the study of mass-limited samples. Alternatively, hyperpolarization schemes based on dynamic nuclear polarization techniques provide strong signal enhancements of the NMR target samples. Here we present a method to effortlessly perform photo-chemically induced dynamic nuclear polarization in microcoil setups to boost NMR signal detection down to sub-picomole detection limits in a 9.4T system (400 MHz 1H Larmor frequency). This setup is unaffected by current major drawbacks such as the use of high-power light sources to attempt uniform irradiation of the sample, and accumulation of degraded photosensitizer in the detection region. The latter is overcome with flow conditions, which in turn open avenues for complex applications requiring rapid and efficient mixing that are not easily achievable on an NMR tube without resorting to complex hardware.
Microbiota derived short chain fatty acids promote histone crotonylation in the colon through histone deacetylases
Authors: Fellows Rachel , Denizot Jérémy , Stellato Claudia , Cuomo Alessandro , Jain Payal , Stoyanova Elena , Balázsi Szabina , Hajnády Zoltán , Liebert Anke , Kazakevych Juri , Blackburn Hector , Corrêa Renan Oliveira , Fachi José Luís , Sato Fabio Takeo , Ribeiro Willian R. , Ferreira Caroline Marcantonio , Perée Hélène , Spagnuolo Mariangela , Mattiuz Raphaël , Matolcsi Csaba , Guedes Joana , Clark Jonathan , Veldhoen Marc , Bonaldi Tiziana , Vinolo Marco Aurélio Ramirez , Varga-Weisz Patrick 
Published: January 2018

AbstractThe recently discovered histone post-translational modification crotonylation connects cellular metabolism to gene regulation. Its regulation and tissue-specific functions are poorly understood. We characterize histone crotonylation in intestinal epithelia and find that histone H3 crotonylation at lysine 18 is a surprisingly abundant modification in the small intestine crypt and colon, and is linked to gene regulation. We show that this modification is highly dynamic and regulated during the cell cycle. We identify class I histone deacetylases, HDAC1, HDAC2, and HDAC3, as major executors of histone decrotonylation. We show that known HDAC inhibitors, including the gut microbiota-derived butyrate, affect histone decrotonylation. Consistent with this, we find that depletion of the gut microbiota leads to a global change in histone crotonylation in the colon. Our results suggest that histone crotonylation connects chromatin to the gut microbiota, at least in part, via short-chain fatty acids and HDACs.
Response speed control of helicity inversion based on a “regulatory enzyme”-like strategy
Authors: Sairenji Shiho , Akine Shigehisa , Nabeshima Tatsuya 
Published: January 2018

AbstractIn biological systems, there are many signal transduction cascades in which a chemical signal is transferred as a series of chemical events. Such successive reaction systems are advantageous because the efficiency of the functions can be finely controlled by regulatory enzymes at an earlier stage. However, most of artificial responsive molecules developed so far rely on single-step conversion, whose response speeds have been difficult to be controlled by external stimuli. In this context, developing artificial conversion systems that have a regulation step similar to the regulatory enzymes has been anticipated. Here we report a novel artificial two-step structural conversion system in which the response speed can be controlled based on a regulatory enzyme-like strategy. In this system, addition of fluoride ion caused desilylation of the siloxycarboxylate ion attached to a helical complex, resulting in the subsequent helicity inversion. The response speeds of the helicity inversion depended on the reactivity of the siloxycarboxylate ions; when a less-reactive siloxycarboxylate ion was used, the helicity inversion rate was governed by the desilylation rate. This is the first artificial responsive molecule in which the overall response speed can be controlled at the regulation step separated from the function step.
Synchrony and Physiological Arousal Increase Cohesion and Cooperation in Large Naturalistic Groups
Authors: Jackson Joshua Conrad , Jong Jonathan , Bilkey David , Whitehouse Harvey , Zollmann Stefanie , McNaughton Craig , Halberstadt Jamin 
Published: January 2018

AbstractSeparate research streams have identified synchrony and arousal as two factors that might contribute to the effects of human rituals on social cohesion and cooperation. But no research has manipulated these variables in the field to investigate their causal – and potentially interactive – effects on prosocial behaviour. Across four experimental sessions involving large samples of strangers, we manipulated the synchronous and physiologically arousing affordances of a group marching task within a sports stadium. We observed participants’ subsequent movement, grouping, and cooperation via a camera hidden in the stadium’s roof. Synchrony and arousal both showed main effects, predicting larger groups, tighter clustering, and more cooperative behaviour in a free-rider dilemma. Synchrony and arousal also interacted on measures of clustering and cooperation such that synchrony only encouraged closer clustering—and encouraged greater cooperation—when paired with physiological arousal. The research helps us understand why synchrony and arousal often co-occur in rituals around the world. It also represents the first use of real-time spatial tracking as a precise and naturalistic method of simulating collective rituals.
Occlusal disharmony-induced stress causes osteopenia of the lumbar vertebrae and long bones in mice
Authors: Shimizu Yasuhiro , Khan Masud , Kato Genki , Aoki Kazuhiro , Ono Takashi 
Published: January 2018

AbstractExcessive exposure to glucocorticoids causes osteoporosis in children and adults. Occlusal disharmony is known to induce an increase in serum corticosteroid levels in murine models, but the influence of occlusal disharmony-induced stress on the bone mass during the growth period has not yet been clarified. The purpose of this study was to investigate whether occlusal disharmony-induced stress decreases bone mass. Five-week-old C57BL/6J male mice were used. A 0.5-mm increase in the vertical height of occlusion was used to induce occlusal disharmony for a period of 7 days. Serum corticosterone levels were significantly higher on post-induction day 7, with radiological evidence of osteopenia of the third lumbar vertebra and long bones of the hind limbs. Osteopenia was associated with a reduction of the mechanical properties of the tibia and femur, with significant suppression of bone formation parameters and an increase in bone resorption parameters, as evaluated by bone histomorphometric analysis of the tibial/femur metaphysis. Our findings at the level of bones were supported by our assessment of serum markers of systemic metabolism. Therefore, occlusal disharmony-induced stress may lead to osteopenia and reduce the mechanical strength of bone through an increase in serum glucocorticoid levels in mice.
Isolation and Characterization of Key Genes that Promote Flavonoid Accumulation in Purple-leaf Tea (Camellia sinensis L.)
Authors: He Xiujuan , Zhao Xuecheng , Gao Liping , Shi Xingxing , Dai Xinlong , Liu Yajun , Xia Tao , Wang Yunsheng 
Published: January 2018

AbstractThere were several high concentrations of flavonoid components in tea leaves that present health benefits. A novel purple-leaf tea variety, ‘Mooma1’, was obtained from the natural hybrid population of Longjing 43 variety. The buds and young leaves of ‘Mooma1’ were displayed in bright red. HPLC and LC-MS analysis showed that anthocyanins and O-Glycosylated flavonols were remarkably accumulated in the leaves of ‘Mooma1’, while the total amount of catechins in purple-leaf leaves was slightly decreased compared with the control. A R2R3-MYB transcription factor (CsMYB6A) and a novel UGT gene (CsUGT72AM1), that were highly expressed in purple leaf were isolated and identified by transcriptome sequencing. The over-expression of transgenic tobacco confirmed that CsMYB6A can activate the expression of flavonoid-related structural genes, especially CHS and 3GT, controlling the accumulation of anthocyanins in the leaf of transgenic tobacco. Enzymatic assays in vitro confirmed that CsUGT72AM1 has catalytic activity as a flavonol 3-O-glucosyltransferase, and displayed broad substrate specificity. The results were useful for further elucidating the molecular mechanisms of the flavonoid metabolic fluxes in the tea plant.
Clinical Outcome of Eosinophilic Airway Inflammation in Chronic Airway Diseases Including Nonasthmatic Eosinophilic Bronchitis
Authors: Cho Jaeyoung , Choi Sun Mi , Lee Jinwoo , Park Young Sik , Lee Sang-Min , Yoo Chul-Gyu , Kim Young Whan , Han Sung Koo , Lee Chang-Hoon 
Published: January 2018

AbstractWe enrolled patients with confirmed sputum eosinophilia who had visited our tertiary referral hospital between 2012 and 2015. We evaluated the incidence and predictors of exacerbations in patients with nonasthmatic eosinophilic bronchitis (NAEB), and investigated predictors of improvement in eosinophilic inflammation in chronic airway diseases with or without persistent airflow limitation. In total, 398 patients with sputum eosinophilia were enrolled. Of these, 152 (38.2%) had NAEB. The incidence rate of exacerbations requiring treatment with antibiotics, systemic corticosteroids, or hospital admission was 0.13 per patient-year (95% CI, 0.06–0.19) in NAEB. Inhaled corticosteroid (ICS) did not affect the risk of exacerbations, even in an analysis of propensity score. One hundred seventy-six patients had chronic airway diseases; in 37 of these (21.0%), sputum eosinophilia had improved at the 1-year follow-up. Patients who had persistent airflow limitation were less likely to show an improvement in eosinophilic inflammation (aOR, 0.26; 95% CI, 0.09–0.77) when they were treated with ICSs for less than 75% of the follow-up days. Exacerbations requiring systemic corticosteroids, antibiotics, or hospitalization did occur, although infrequently, in NAEB patients. Among patients with chronic airway diseases, those with persistent airflow limitation were less likely to show an improvement in eosinophilic airway inflammation.
Flt3 ligand expands bona fide innate lymphoid cell precursors in vivo
Authors: Parigi Sara M. , Czarnewski Paulo , Das Srustidhar , Steeg Christiane , Brockmann Leonie , Fernandez-Gaitero Sara , Yman Victor , Forkel Marianne , Höög Charlotte , Mjösberg Jenny , Westerberg Lisa , Färnert Anna , Huber Samuel , Jacobs Thomas , Villablanca Eduardo J. 
Published: January 2018

AbstractA common helper-like innate lymphoid precursor (CHILP) restricted to the innate lymphoid cells (ILC) lineage has been recently characterized. While specific requirements of transcription factors for CHILPs development has been partially described, their ability to sense cytokines and react to peripheral inflammation remains unaddressed. Here, we found that systemic increase in Flt3L levels correlated with the expansion of Lineage (Lin)negα4β7+ precursors in the adult murine bone marrow. Expanded Linnegα4β7+ precursors were bona fide CHILPs as seen by their ability to differentiate into all helper ILCs subsets but cNK in vivo. Interestingly, Flt3L-expanded CHILPs transferred into lymphopenic mice preferentially reconstituted the small intestine. While we did not observe changes in serum Flt3L during DSS-induced colitis in mice or plasma from inflammatory bowel disease (IBD) patients, elevated Flt3L levels were detected in acute malaria patients. Interestingly, while CHILP numbers were stable during the course of DSS-induced colitis, they expanded following increased serum Flt3L levels in malaria-infected mice, hence suggesting a role of the Flt3L-ILC axis in malaria. Collectively, our results indicate that Flt3L expands CHILPs in the bone marrow, which might be associated with specific inflammatory conditions.