bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mammalian Mss51 is a Skeletal Muscle-Specific Gene Modulating Cellular Metabolism
Authors: Moyer Adam L. , Wagner Kathryn R. 
Published: November 2015

Background: The transforming growth factor β (TGF-β) signaling pathways modulate skeletal muscle growth, regeneration, and cellular metabolism. Several recent gene expression studies have shown that inhibition of myostatin and TGF-β1 signaling consistently leads to a significant reduction in expression of Mss51, also named Zmynd17. The function of mammalian Mss51 is unknown although a putative homolog in yeast is a mitochondrial translational activator. Objective: The objective of this work was to characterize mammalian MSS51. Methods: Quantitative RT-PCR and immunoblot of subcellular fractionation were used to determine expression patterns and localization of Mss51. The CRISPR/Cas9 system was used to reduce expression of Mss51 in C2C12 myoblasts and the function of Mss51 was evaluated in assays of proliferation, differentiation and cellular metabolism. Results: Mss51 was predominantly expressed in skeletal muscle and in those muscles dominated by fast-twitch fibers. In vitro, its expression was upregulated upon differentiation of C2C12 myoblasts into myotubes. Expression of Mss51 was modulated in response to altered TGF-β family signaling. In human muscle, MSS51 localized to the mitochondria. Its genetic disruption resulted in increased levels of cellular ATP, β-oxidation, glycolysis, and oxidative phosphorylation. Conclusions: Mss51 is a novel, skeletal muscle-specific gene and a key target of myostatin and TGF-β1 signaling. Unlike myostatin, TGF-β1 and IGF-1, Mss51 does not regulate myoblast proliferation or differentiation. Rather, Mss51 appears to be one of the effectors of these growth factors on metabolic processes including fatty acid oxidation, glycolysis and oxidative phosphorylation.
Adult Onset Leigh Syndrome in the Intensive Care Setting: A Novel Presentation of a C12orf65 Related Mitochondrial Disease
Authors: Wesolowska Maria , Gorman Grainne S. , Alston Charlotte L. , Pajak Aleksandra , Pyle Angela , He Langping , Griffin Helen , Chinnery Patrick F. , Miller James A.L. , Schaefer Andrew M. , Taylor Robert W. , Lightowlers Robert N. , Chrzanowska-Lightowlers Zofia M. 
Published: November 2015

Background: Mitochondrial disease can present at any age, with dysfunction in almost any tissue making diagnosis a challenge. It can result from inherited or sporadic mutations in either the mitochondrial or the nuclear genome, many of which affect intraorganellar gene expression. The estimated prevalence of 1/4300 indicates these to be amongst the commonest inherited neuromuscular disorders, emphasising the importance of recognition of the diagnostic clinical features. Objective: Despite major advances in our understanding of the molecular basis of mitochondrial diseases, accurate and early diagnoses are critically dependent on the fastidious clinical and biochemical characterisation of patients. Here we describe a patient harbouring a previously reported homozygous mutation in C12orf65, a mitochondrial protein of unknown function, which does not adhere to the proposed distinct genotype-phenotype relationship. Methods: We performed clinical, biochemical and molecular analysis including whole exome sequencing on patient samples and cell lines. Results: We report an extremely rare case of an adult presenting with Leigh-like disease, in intensive care, in the 5th decade of life, harbouring a recessively inherited mutation previously reported in children. A global reduction in intra-mitochondrial protein synthesis was observed despite normal or elevated levels of mt-RNA, leading to an isolated complex IV deficiency. Conclusions: All the reported C12orf65 mutations have shown an autosomal recessive pattern of inheritance. Mitochondrial disease causing mutations inherited in this manner are usually of early onset and associated with a severe, often fatal clinical phenotype. Presentations in adulthood are usually less severe. This patient’s late adulthood presentation is in sharp contrast emphasising the clinical variability that is characteristic of mitochondrial disease and illustrates why making a definitive diagnosis remains a formidable challenge.
Efficacy and safety of goserelin combined with adjuvant chemotherapy in premenopausal women with breast cancer
Authors: Wang Yang , Zhu Zheng Fei , Pan Wei Jun , Xu Zhe 
Published: November 2015

<p class="Abstract">This study aims to evaluate the efficacy and safety of goserelin combined with chemotherapy for premenopausal women with breast cancer. Literatures were extracted from databases including Excerpta Medica Database, Springer, Pubmed, China National Knowledge Infrastructure and Chinese Biological Medicine from their inception up to May 2014. The main efficacy measures were 5 years overall survival (OS), 10 years OS, 5 years disease free survival and 5 years progress free survival. Ten randomized comparison clinical trials were eligible in this study. The result showed that goserelin combined with chemotherapy group can improve the survival rate and decrease the incidence of arthralgia in postmenopausal breast cancer patients, respectively, compared to the control group. However, they can increase the occurrence of vomiting during the chemotherapy process. Compared with the simple chemotherapy, goserelin combined with chemotherapy can provide benefits for premenopausal women with breast cancer on improving the survival rate and reducing arthralgia.</p><p> </p>
Análise qualitativa de tensões em estruturas sólidas acopladas a amostras de resina fotoelástica
Authors: Silva Sidney Leal , Lima Antônio Valdinei , Da Silva Luan Neves , Da Silva Charles Rodrigues , Gomes Carlos , Santos Esther Gehrti Regis , Batista Henrique Sampaio , Ta Gein Lindaci Aparecida 
Published: November 2015

<p>Nesse trabalho foram estudadas amostras de resina fotoelástica, pela técnica de fotoelasticidade, envolvendo dois tipos de materiais: alumínio e cobre. As franjas de interferência observadas foram comparadas e contrastadas com as de uma amostra padrão. O instrumento utilizado para captura dos dados foi um polariscópio linear.<strong></strong></p><p>Foi realizada uma análise qualitativa de dados em função das observações feitas com os dados colhidos através de uma câmera fotográfica. Verificou-se que houve mudanças nos padrões das franjas em todas as amostras. As principais diferenças foram: (i) nitidez nas cores; (ii) curvatura e (iii) quantidade de franjas.</p><p>Com essas observações, concluiu-se que é possível estudar determinadas propriedades desses materiais, como exemplo a resistência mecânica, porém através de processos que envolvem análises quantitativas das observações, apontando, assim, para uma continuidade desse trabalho. </p>
A ocupação em Joinville/SC e o papel da gestão municipal para mitigação de danos causados por inundações
Authors: Muller Cristiane Regina , Oliveira Francisco Henrique de , Schardosim Patrícia Royes 
Published: November 2015

<p style="text-align: justify;"><span style="font-size: 11.0pt;">A origem da ocupação no município de Joinville/SC desenvolveu-se na planície do Rio Cachoeira onde as inundações ocorrem tanto pelas cheias do rio quanto pela ação das marés. No entanto, mesmo com o conhecimento desta situação e sendo esta recorrente, o centro urbano do município estruturou-se neste local. Os dados e as informações apresentadas foram adquiridos por meio de pesquisa bibliográfica e no âmbito da Chamada Pública Prevenção de Desastres Naturais n° 10/2009 - Fundação de Amparo a Pesquisa e Inovação de Santa Catarina – FAPESC. Com isto o presente trabalho faz uma abordagem ligada a caracterização física, histórica e socioeconômica da área de estudo, bem como avalia os instrumentos de ordenamento territorial que o município dispõe com vistas a mitigar os danos causados pelas constantes inundações.</span></p>
Questões sobre a integração de novos meios de mobilidade ao plano de mobilidade urbana e transporte de Curitiba
Authors: Martins Andrea Martinesco Coelho 
Published: November 2015

<div>A análise do Plano de Mobilidade Urbana e Transporte Integrado da Prefeitura Municipal de Curitiba </div><div>demonstra a grande preocupação dos agentes públicos com o desenvolvimento de Políticas Públicas </div><div>no campo da mobilidade urbana, envolvendo, para o que interessa no presente trabalho, o transporte </div><div>coletivo e o transporte comercial metropolitano. Nesse sentido, foi realizado estudo sobre os novos </div><div>usos relacionados aos serviços de mobilidade compartilhada desenvolvidos na Europa, em especial na </div><div>França, para identificação de alternativas viáveis aos problemas de saturação do sistema viário nas </div><div>áreas urbanas e suburbanas de Curitiba, visando ao transporte urbano sustentável. Serão apresentados </div><div>dois sistemas alternativos aos descritos no PlanMob Curitiba – covoiturage e autopartage –,</div><div>introduzidos na Europa desde a década de 1980. Serão estudadas as características de cada sistema e </div><div>avaliadas as estatísticas francesas. O resultado da pesquisa indicou que, naquele país, pela crescente </div><div>demanda por alternativas ao uso do petróleo, associada às mudanças climáticas, bem como à saturação </div><div>das redes de mobilidade, há um esforço multidisciplinar na formação e adoção de novos sistemas de </div><div>mobilidade eco-sustentáveis, que passa pelo desenvolvimento do veículo elétrico, também chamado de </div><div>carro verde (com emissão “zero” de carbono), a delegação da condução e a conectividade, que vão </div><div>além do proposto nesse trabalho. </div>
Cálculo dos escores e estatística descritiva do WHOQOL-8 por meio do Microsoft Excel
Authors: Pedroso Bruno , Ferreira Bruna Maria Palotino 
Published: November 2015

Haja vista a limitação ocasionada pela obrigatoriedade de utilização do software <em>Statistical Package for Social Sciences</em> (SPSS) na utilização dos instrumentos WHOQOL, o objetivo do presente trabalho foi construir uma ferramenta a partir do software Microsoft Excel para o cálculo dos escores e estatística descritiva do WHOQOL-8. Para testar a fidedignidade da ferramenta proposta, fora realizada uma aplicação do WHOQOL-8 em 56 indivíduos. Os cálculos dos resultados foram realizados por meio da ferramenta construída no presente estudo e também por meio da sintaxe SPSS proposta pelos desenvolvedores do WHOQOL-8. Os resultados retornados pela ferramenta foram comparados com os resultados retornados pelo SPSS, tendo havido 100% de coincidência entre ambos. Conclui-se que a partir do presente estudo foi possível a disponibilização de uma ferramenta alternativa, fidedigna à sintaxe original, que facilita o acesso aos resultados de aplicações do WHOQOL-8, possibilitando a obtenção dos resultados deste por meio de uma interface simples e sem a necessidade de aquisição de softwares para fins específicos e que requerem conhecimentos avançados de utilização.
Comparação do nível de Qualidade de Vida dos indivíduos com Síndrome de Down praticantes e não praticantes de atividade física
Authors: Melo Aline Cristina Sá de , César Eurico Peixoto 
Published: November 2015

<p><strong>OBJETIVO: </strong>Comparar o nível de qualidade de vida dos indivíduos com Síndrome de Down praticantes e não praticantes de atividade física.</p><p><strong>MÉTODO:</strong> O estudo é do tipo transversal, comparativo e de caráter qualitativo. A amostra foi composta por oito indivíduos com Síndrome de Down de ambos os sexos divididos em dois grupos, praticantes (20,2 ± 8,7 anos) e não praticantes (25,2 ± 2,0 anos) de atividade física, atendidos na Associação de Pais e Amigos dos Excepcionais (APAE), na cidade de Juiz de Fora/MG. Para realização da pesquisa foi aplicado o Questionário de Qualidade de Vida SF-36 (<em>Medical Outcomes Study 36 – Item Short Form Health Survey</em>), traduzido e homologado para a realidade brasileira. O questionário foi respondido pelos pais e/ou responsáveis dos indivíduos que apresentavam a síndrome, devido ao grau de deficiência de cada um deles.</p><p><strong>RESULTADOS: </strong>Dentre os oito domínios avaliados na pesquisa, foram encontradas diferenças significativas apenas para Capacidade Funcional (75 vs. 25; p=0,042) e Saúde Mental (86 vs. 60; p=0,028), com resultados favoráveis ao grupo praticante de atividade física em relação ao grupo de não praticantes.</p><p><strong>CONCLUSÃO:</strong> Numa avaliação geral, verificou-se que a prática de atividade física garantiu melhores resultados nos domínios Capacidade Funcional e Saúde Mental de indivíduos com Síndrome de Down, refletindo, assim, em uma melhor qualidade de vida dessas pessoas. </p>
Un autre regard sur la campagne est possible !
Authors: Bourdeau Vincent , Cottin-Marx Simon 
Published: November 2015

La recherche scientifique offre un tableau très urbano-centré du monde rural. Dans cet entretien, la ruraliste Georgette Zrinscak, maîtresse de conférence en géographie à l’université Paris 1, invite à abandonner les clichés citadins, à décoloniser nos imaginaires de la campagne fantasmée. Changer de posture nous permet de voir que la campagne garde ses spécificités, partout en Europe elle est traversée par des évolutions très hétérogènes.
L’affect à l’école : projet thérapeutique partagé d’un élève en difficulté
Authors: Allegra Julie 
Published: November 2015

La relation enseignants-élève à l’école est le lieu d’émergence privilégié d’intenses vécus affectifs. Quels facteurs facilitent la construction d’un bon espace émotionnel où se mettre en jeu pour apprendre et enseigner, et lesquels constituent, au contraire, une entrave à la possibilité d’apprendre et de vivre une harmonieuse relation d’apprentissage ? Les hypothèses formulées ici se fondent sur l’expérience de l’auteure, psychothérapeute d’enfants, ayant accompagné, pendant 5 ans, l’équipe éducative d’une école primaire de Rome d’un jeune enfant en grande difficulté scolaire, Raphaël, suivi parallèlement en psychothérapie. En nous penchant sur les conflits et les mécanismes de projections défensives circulant entre les enseignantes, les parents et l’enfant, nous verrons comment les réunions scolaires avec la psychothérapeute ont permis aux différents interlocuteurs d’entamer un dialogue autour du vécu complexe de l’enfant et d’internaliser une fonction d’étayage. La reconnaissance et l’élaboration de certains affects et aspects moins conscients du monde interne ont permis la transformation d’une expérience de souffrance à l’école en expérience positive de soi, susceptible d’alimenter la relation d’éducation et de la rendre plus gratifiante pour tous les acteurs en jeu.
El imperio medieval hispánico y la Chronica Adefonsi Imperatoris
Authors: Gambra Gutiérrez Andrés 
Published: November 2015

La Chronica Adefonsi Imperatoris es un relato histórico de extraordinario interés. Redactado en el entorno de Alfonso VII, propone al lector una visión muy sopesada de la trayectoria del emperador, dirigida a magnificar su figura. La Crónica sitúa al monarca leonés al frente de un imperio de carácter feudo-vasallático, apto para asumir la dirección de las entidades políticas que, bajo el signo de la pluralidad, proliferaban en la Península a mediados del siglo XII; un imperio sin relación con el de su abuelo Alfonso VI, de inspiración neogótica. El cronista localiza la institución de ese imperio de nuevo cuño en el concilio de León en mayo de 1135, donde Alfonso VII fue coronado emperador, versión de los hechos que ha condicionado la imagen de su trayectoria histórica. En estas páginas se intenta demostrar que la realidad fue menos lineal y que el imperio de Alfonso VII atravesó por etapas distintas, de modo que solo en una fase tardía cabe identificar su configuración con la que le atribuye la CAI.
A Good Death in Ratnapura : A Qualitative Study
Authors: Perera M. C. , Ramadasa U. , Chandrasiri G. M. L. W. , Thaibudeen M. A. , Kalupahana K. M. S. N 
Published: November 2015

Introduction: One of the most important goals of medical care in patients having a life limiting illness is achieving a ‘‘good death’’. To achieve this, components of a good death should be identified.Objectives: To identify the components of a good death in Ratnapura, Sri Lanka.Methodology: A qualitative, prospective serial study was done. The study sample included patients diagnosed as having life limiting illness and being followed up at the ENT, Palliative care and Oncology units at General Hospital Ratnapura.Results: There were 42 participants in the study. Thirty five (83.33%) of the patients had cancer and 7(16.67%) had non cancer life limiting illnesses. The study identified attributes which fall to 15 categories as common components of ‘‘good death’’.Conclusion: The main categories of good death in Ratnapura, Sri Lanka, were having a good family relationship, having a good relationship with medical staff, Not being aware of death, Fighting against the disease, Dying at ones favourite place, Having faith, Maintaining hope, Maintaining dignity, Freedom from Pain, Physical & Psychological symptoms, Not being a burden to others and Maintaining sense of control. Home was considered the best place to die.Ceylon Journal of Otolaryngology Vol.4(1) 2015 pp. 20-25
Effect of Instrumentation Techniques, Irrigant Solutions and Artificial accelerated Aging on Fiberglass Post Bond Strength to Intraradicular Dentin
Authors: Soares Carlos Jose , Estrela Carlos , Santana Fernanda Ribeiro , Silva Júlio Almeida , Alencar Ana Helena Gonçalves , Renovato Sara Rodrigues , Lopes Lawrence Gonzaga 
Published: November 2015

ABSTRACT

Objective
To evaluate the effect of instrumentation techniques, irrigant solutions and specimen aging on fiberglass posts bond strength to intraradicular dentine.

Materials and methods
A total of 120 bovine teeth were prepared and randomized into control and experimental groups resulting from three study factors (instrumentation techniques, irrigant solutions, specimen aging). Posts were cemented with RelyX U100. Samples were submitted to push-out test and failure mode was evaluated under a confocal microscope.

Results
In specimens submitted to water artificial aging, nickel-titanium rotary instruments group presented higher bond strength values in apical third irrigated with NaOCl or chlorhexidine. Irrigation with NaOCl resulted in higher bond strength than ozonated water. Artificial aging resulted in significant bond strength increase. Adhesive cement-dentin failure was prevalent in all the groups.

Conclusion
Root canal preparation with NiTi instruments associated with NaOCl irrigation and ethylenediaminetetra acetic acid (EDTA) increased bond strength of fiberglass posts cemented with self-adhesive resin cement to intraradicular dentine. Water artificial aging significantly increased postretention.

Clinical significance
The understanding of factors that may influence the optimal bond between post-cement and cementdentin are essential to the success of endodontically treated tooth restoration.

How to cite this article
Santana FR, Soares CJ, Silva JA, Alencar AHG, Renovato SR, Lopes LG, Estrela C. Effect of Instrumentation Techniques, Irrigant Solutions and Artificial accelerated Aging on Fiberglass Post Bond Strength to Intraradicular Dentin. J Contemp Dent Pract 2015;16(7): 523-530.
Oral Paleomicrobiology: Study of Ancient Oral Microbiome
Authors: Baroudi Kusai , Dagli Rushabh , Dagli Namrata , Tarakji Bassel 
Published: November 2015

ABSTRACT

Background
Paleomicrobiology is a special branch of micropaleontology concerned with the study of bacterial fossils. We have used the term ‘oral paleomicrobiology’, as in this review we have focused on the ancient oral microflora. Recently, dental calculus and dental pulp have been identified as rich sources of ancient microbial DNA. Study of this ancient genetic material opens a new door to the ancient world. This review gives an overview of history of ancient DNA research, various techniques of analyzing ancient DNA in dental calculus and dental pulp, and the implications of the oral paleomicrobiology.

Materials and methods
A comprehensive literature search was performed in the following databases—pubmed, medline and google scholar for studies published before 10 April, 2015. The following keywords were used—‘ancient DNA’, ‘ancient oral flora, ‘oral paleomicrobiology’ and ‘oral microbiome’, ‘16S rRNA sequencing’. To obtain additional data, a manual search was performed using the reference lists of selected articles.

Result
As a result of literature search, 27 articles were found in pubmed, 12 in google scholar and one in medline. Eight more articles were selected from the reference list of selected articles.

Conclusion
The combination of microbiology and paleontology has brought a revolution in the study of human evolution and microbial communities. The naturally well-preserved samples of microbial DNA from dental pulp and microbial colonies trapped in dental calculus are a potential source of microbial genetic material, which will prove invaluable in resolving mysteries of the past. This may be a beginning of a new era of oral paleomicrobiology, which will contribute in our studies about prevention of disease by establishing symbiosis between human beings and their microbiome.

How to cite this article
Dagli N, Dagli R, Baroudi K, Tarakji B. Oral Paleomicrobiology: Study of Ancient Oral Microbiome. J Contemp Dent Pract 2015;16(7):588-594.
Comparative Evaluation of Fracture Resistance and Mode of Failure of Zirconia and Titanium Abutments with Different Diameters
Authors: Ghodsi Safoura , Shabanpour Reza , Mousavi Niloufar , Alikhasi Marzieh 
Published: November 2015

ABSTRACTAimThe purpose of the current study was to compare the fracture resistance and mode of failure of zirconia and titanium abutments with different diameters.Materials and methodsFourteen groups of abutments including prefabricated zirconia, copy-milled zirconia and titanium abutments of an implant system (XiVE, Dentsply) were prepared in different diameters. An increasing vertical load was applied to each specimen until failure occurred. Fracture resistance was measured in each group using the universal testing machine. Moreover, the failure modes were studied and categorized as abutment screw fracture, connection area fracture, abutment body fracture, abutment body distortion, screw distortion and connection area distortion. Groups were statistically compared using univariate and post-hoc tests. The level of statistical significance was set at 5%.ResultsFabrication method (p = 0.03) and diameter (p < 0.001) had significant effect on the fracture resistance of abutments. Fracture resistance of abutments with 5.5 mm diameter was higher than other diameters (p < 0.001). The observed modes of failure were dependent on the abutment material as well. All of the prefabricated titanium abutments fractured within the abutment screw. Abutment screw distortion, connection area fracture, and abutment body fracture were the common failure type in other groups.ConclusionDiameter had a significant effect on fracture resistance of implant abutments, as abutments with greater diameters were more resistant to static loads. Copy-milled abutments showed lower fracture resistance as compared to other experimental groups.Clinical significanceAlthough zirconia abutments have received great popularity among clinicians and even patients selecting them for narrow implants should be with caution.How to cite this articleShabanpour R, Mousavi N, Ghodsi S, Alikhasi M. Comparative Evaluation of Fracture Resistance and Mode of Failure of Zirconia and Titanium Abutments with Different Diameters. J Contemp Dent Pract 2015;16(8):613-618.