bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of Compartment Syndrome Following Peri-Pandemic Intervention for Non-traumatic Acute Limb Ischemia
Authors: Chihade Deena B. , Williams Zachary E. , Wainwright Brandon S. , Shaw Palma M. 
Published: October 2024

Objective During the pandemic, our institution anecdotally observed a significant proportion of acute limb ischemia (ALI) patients developing compartment syndrome (CS) following revascularization compared to pre-pandemic rates. To determine whether this perceived increase was occurring globally, we utilized the TriNetX database to evaluate the incidence of CS secondary to ALI intervention in both the pre-pandemic (2017-2019) and pandemic eras (2020-2022). Methods We conducted a multicenter query using the TriNetX global research network for ALI patients receiving treatment. Incidence of CS diagnosis within 1 calendar day of ALI intervention was calculated for each era. Demographics and comorbidities were then compared between CS and non-CS patients within each era. Risk of adverse outcomes within 30 days of CS diagnosis was also determined for each era, including mortality, major amputation, and re-intervention. Results The pre-pandemic cohort contained 7736 patients while the pandemic era cohort included 8,306, for 16,042 total patients. A significant increase in CS incidence (risk ratio (RR) = 1.23, P = 0.0026) was demonstrated within the pandemic era. An increased prevalence of comorbidities such as dyslipidemia (pre-pandemic: P = 0.0022; pandemic: P = 0.0026) and peripheral vascular disease ( P < 0.0001, both eras) was observed in the non-CS cohort within both eras. 30-day mortality was significantly increased in CS patients (pre-pandemic: RR = 3.057; pandemic: RR = 2.710; P < 0.0001 both eras) compared to non-CS patients. CS patients were more likely to receive major amputation (pre-pandemic: RR = 3.734; pandemic: RR = 2.809; P < 0.0001 both eras) and/or re-intervention within 30 days (pre-pandemic: RR = 1.871, P < 0.0001; pandemic: RR = 1.370, P = 0.0218) over non-CS patients. Conclusions The incidence of CS following revascularization for ALI rose worldwide during the pandemic. Patients who developed CS are younger with fewer comorbidities than non-CS patients. Despite a more favorable comorbid profile, CS patients demonstrate significantly higher rates of adverse outcomes. Further investigation is necessary to determine the specific underlying mechanisms driving this increased incidence in CS among ALI patients.
Re-designing the experience of transition into nursing homes: A Singapore study
Authors: Lim Benjamin Wei Ming , Xue Agnes Lishan , Toh Irene , Lim Sok Mui 
Published: October 2024

Introduction Transition of senior adults from community into nursing homes (NH) is a challenging event for both the senior adults and their family. Even with the introduction of new service models, studies continued to advocate that the navigation of services should remain simple and streamlined. In relation to the ‘wicked problem’ that persist, design thinking methodology and tools can be adopted to address the unacceptably huge gap between the services that NH operators aim to deliver and the actual service that is delivered. The study aims to understand the experience of senior adults transitioning into NH from the design thinking approach. Methods A phenomenological research design was used with 10 senior adults recruited. The protocol consisted of three 8-h sessions, in-depth observations of the participants in an NH environment and a 45-min semi-structured interview with the participants to understand their experience of transition into a NH. Results Four distinct phases were unravelled to illustrate the transition process. Four personas were also created to represent the types of senior adults who are likely to transition into a NH. Main factors that influence the experience of transition include (1) emotions of the senior adults, (2) support provided during the transition, and (3) frequency of visitation by others. Conclusion The study validated the complexity of transition into a NH. Using the design thinking methodology and tools, such as interviewing with empathy and the creation of personas, findings can be used to develop a service model that addresses the challenges of seniors’ transition into a NH.
Comparison of intrathecal fentanyl as an adjunct in spinal anaesthesia to ultrasound-guided quadratus lumborum block as an effective postoperative analgesia for Caesarean section surgery
Authors: Patnaik Subhasish , Singh Shalendra , Dash Lalita Kumar , Sharma Arunav , Chandran Anand Naveen , Taank Priya 
Published: October 2024

Background: Postoperative pain in patients, if dealt with inadequately, has been a significant cause of morbidity. The present study compared the postoperative analgesic efficacy of intrathecal fentanyl and ultrasound-guided quadratus lumborum block following Caesarean surgery. Methods: A prospective randomised controlled study was planned for parturients who underwent Caesarean surgery under spinal anaesthesia. Patients received bupivacaine (10mg) and 25µg of intrathecal fentanyl in the spinal anaesthesia (group intrathecal fentanyl, n = 30) or 20mL of 0.375% ropivacaine bilateral quadratus lumborum block (group quadratus lumborum; n = 30) after surgery. The visual analogue scale score, quality of recovery-15 score and incidence of ill effects were recorded. Results: The postoperative haemodynamic parameters were comparable between the two groups. The visual analogue scale score at different time intervals decreased after the quadratus lumborum block (p < 0.05). The mean global quality of recovery score was better in the quadratus lumborum group (p < 0.001). In the quadratus lumborum group, the mean time to first ambulation was lower than that in the intrathecal fentanyl group (p < 0.05). The requirement for first-rescue analgesia was earlier in the intrathecal fentanyl group (4.67 ± 0.72) than in the quadratus lumborum group (4.92 ± 0.88). Conclusion: Intrathecal fentanyl and quadratus lumborum block had effective postoperative analgesic effects on Caesarean surgery patients. However, the quadratus lumborum block group exhibited better analgesia and early ambulation than the intrathecal fentanyl group, with an improved quality of recovery.
Affordance trajectories and the usefulness of online records access among older adults in Sweden
Authors: Huvila Isto , Rexhepi Hanife , Moll Jonas , Ghorbanian Zolbin Maedeh , Blease Charlotte , Bärkås Annika , Åhlfeldt Rose-Mharie , Hagström Josefin , Kane Bridget , Scandurra Isabella , Hägglund Maria , Klein Gunnar O. , Wang Bo , Kharko Anna 
Published: October 2024

Objective The current understanding of the breadth of individual differences in how eHealth technologies are perceived as useful for different purposes is incomprehensive. The aim/purpose of the study is to improve the understanding of diverse perceptions of the usefulness of technologies by exploring older adults’ use of their patient-accessible electronic health records (PAEHRs). Methods The study applies and extends Affordance Theory based on an empirical analysis of data from the NORDeHEALTH 2022 Patient Survey on attitudes toward PAEHR in Norway, Sweden, Finland, and Estonia. Responses from 3964 participants in Sweden, aged 65 + years were analysed. Data included demographics and agreement ratings to reasons for using PAEHR. To analyse variation in the reasons for using PAEHR, group comparisons were conducted based on gender (male/female), age group (65–74, 75–84 and 85+) and earlier encouragement to use PAEHR. Results Overall, the findings suggest that PAEHRs have multiple parallel affordance trajectories and affordance potencies that actualise differently depending on needs. The top reasons, pointing to both orientational and goal-oriented affordances for using PAEHR, were improving understanding of health issues, getting an overview of medical history/treatment and ensuring understanding of what the doctor said. Men reported more often sharing information with relatives or friends as a reason to access PAEHR. Women were more inclined, albeit similarly to men less frequently, to read their PAEHR for detecting errors. Age had little influence on reasons for using PAEHR. Conclusions The study applies and extends Affordance Theory in the context of older adults’ PAEHR use based on findings from the largest national investigation of reasons for older users to access PAEHR in Sweden demonstrating the applicability of the theory in improving the understanding of the diversity of individual perceptions on eHealth technologies.
Beyond the filter: Impact of popularity on the mental health of social media influencers
Authors: Azayem Ala’a K. , Nawaz Faisal A. , Jeyaseelan Lakshmanan , Kair Hawk M. , Sultan Meshal A. 
Published: October 2024

Objective This study examines the emotional state and interpersonal relationships of social media influencers, focusing on the psychological effects of their popularity and engagement. Despite increased awareness of mental health issues, influencers remain underrepresented in research. Barriers such as low mental health literacy, stigma, access issues, and the pressure to maintain virtual personas highlight the need for this investigation. This research addresses these gaps by systematically examining the impact of social media on influencers’ mental health. Methods An online survey was conducted using the positive and negative affect schedule (PANAS) and the Relationship Structures Questionnaire (ECR-RS). The target audience was social media influencers from various countries, with a specific focus on the United Arab Emirates (UAE). Participants were recruited from 2 November 2022, to 1 March 2023, through email, SMS, and direct messages on social media platforms. Statistical analyses included t-tests, ANOVA, and Pearson correlation coefficients. Results A total of 161 social media influencers completed the survey. A significant association was found between extended social media usage and heightened negative emotions among influencers spending more than 5 hours daily on these platforms ( p < .05). Influencers earning less than $10,000 from social media reported the lowest negative feeling scores. However, higher income levels correlated with increased relationship avoidance and anxiety scores (e.g., p < .01 for avoidance and anxiety from parents and best friends). Additionally, an increase in the number of followers was associated with increased negative emotions ( p = .001). Conclusion Our study provides compelling insights into the well-being, emotions, and relationship quality of social media influencers. It underscores the urgency of prioritizing their mental well-being on a global scale, helping them navigate the challenges of their digital careers while maintaining a positive impact on their audiences.
Data professionals’ attitudes on data privacy, sharing, and consent in healthcare and research
Authors: Kaplow Katya , Downey Max , Stewart Darren , Massie Allan B. , Motter Jennifer D. , Taylor Lauren , Massarelli John , Matalon Taylor , Sidoti Carolyn , Levan Macey L. , Parent Brendan 
Published: October 2024

Objective Individuals who work on health data systems and services are uniquely positioned to understand the risks of health data collection and use. We designed and conducted a survey assessing the perceptions of those who work with health data around health data consent, sharing, and privacy practices in healthcare and clinical research. Methods A 43-item online survey was distributed via a market research firm to individuals (18+) who work with health data in the United States from March to April 2023. Descriptive statistics were calculated for all variables. Associations with demographic variables were assessed using Pearson's X2 tests and ordinal logistic regression. Results Most of our respondents (61.7%) reported that they would trust people to use their health data across various sectors, but more respondents trusted those working in academic medical research (86.5%) and healthcare offices (89.9%) compared to those working in industry (68.2%). Despite this reported trust, a strong majority believed that individuals should have complete control over their health data (97.3%), specific consent should be obtained for each use of their health data (92.0%), and that there should be higher standards of consent and privacy for health records data than other types of data (93.7%). Conclusions Based on our findings, we might infer that people who work with health data generally trust institutions across sectors to protect their health data. However, many would prefer to have complete control over who has access to their health data and how it is used. These insights should be explored further through qualitative studies.
Postoperative Rehabilitation and Return-to-Sport Criteria After Distal Triceps Rupture Repair: A Scoping Review
Authors: Phelps Brian M. , Fomunung Clyde , Singer William , George Andrew , Jack Robert A. 
Published: October 2024

Background: Distal triceps rupture is a rare injury that is commonly surgically repaired to improve elbow strength and function. Most patients are active and have goals to return to specific activities and sports. There is no gold standard rehabilitation protocol for return to sport (RTS) after distal triceps repair. Purpose: To identify in the literature any criteria used for RTS after distal triceps repair. Study Design: Scoping review; Level of evidence, 4. Methods: Under PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines, level 1 to 4 studies that examined distal triceps repair and had a minimum follow-up duration of 12 months were deemed eligible for study inclusion. Exclusion criteria encompassed studies that employed nonoperative treatment, lacked specific RTS criteria, or involved revision cases. The selected studies underwent assessment regarding RTS outcomes, timelines, rates, and methodological quality. Results: Of the initial 378 unique studies, 6 studies (including 116 patients involved in 16 different sports) met the inclusion criteria. Four of the studies reported RTS rates, with a mean of 89.3%. The duration of time for returning to sport was reported in 2 studies and varied from 4 to 6 months. The parameters most emphasized in the rehabilitation protocols were progressive range of motion exercises, followed by early immobilization and progressive strengthening exercises. Supervised therapy was reported in only 2 (33%) studies. Although all studies included a rehabilitation protocol and 5 studies included a timeline for RTS, only 1 study provided subjective criteria, and no studies utilized specific objective measurement criteria. The overall study quality was fair. Conclusion: This review demonstrated that the overall RTS rate after distal triceps repair was high (89.3%), with no consensus on RTS criteria. Most studies included initial immobilization followed by progressive range of motion and strengthening. Timing after surgery was used as a measure to RTS in the minority of studies and varied between 4 and 6 months. Further research is needed to develop functional and performance-based metrics to better standardize RTS criteria and rehabilitation protocols.
Predictors of Academic and Clinical Stress Among Nursing Students
Authors: Mohamed Nermen Abdelftah , Ali Samah Osman , Ehrahim Elturabi Elsayed Elkhider , Ahmed Amany Lotfy , Wahba Ahmed Mohamed 
Published: October 2024

Introduction Nursing students experience significant academic and clinical stress due to challenging coursework, assessments, and educational demands, as well as the pressures associated with gaining practical healthcare experience. These challenges can lead to anxiety, fear of making errors, and concerns about patient well-being, ultimately impacting students’ mental health and their preparation for a nursing career. Objective assesses the predictors of academic and clinical stress among nursing students. Method A cross-sectional study design was used with a convenience sample of 113 nursing students. The study team conducted the study at the Faculty of Applied Medical Science, Nursing Department at Prince Sattam bin Abdulaziz University. The researchers used three instruments for data collection and asked all nurse students to complete the online questionnaires, which utilized perceived clinical settings and academic stress. Results The mean age of the studied nursing students was 21.2 years old. The study observed students had moderate perceived stress levels furthermore, the total levels of the academic stress domain's mean and standard deviation in students were 90.5 ± 32. Also, a significant relationship between perceived stress and clinical setting hours per week in nursing students, and hospital clinical setting type in nursing students. There was a correlation between academic stress and the ages of students. Students’ academic stress was statistically significantly correlated with their educational level. Conclusion Academic and clinical stress significantly impacts nursing students; the most common reasons that increase stress are fear of making mistakes and harming the patient. Recommendation Accordingly, the study advocates for developing educational strategies and a structured course curriculum before student nurses are sent to clinical areas to enhance their capabilities and reduce academic and clinical stress.
Ascorbate deficiency increases quiescence and self-renewal in hematopoietic stem cells and multipotent progenitors
Authors: Comazzetto Stefano , Cassidy Daniel L. , DeVilbiss Andrew W. , Jeffery Elise C. , Ottesen Bethany R. , Reyes Amanda R. , Paul Animesh , Bansal Suraj , Xie Stephanie Z. , Muh Sarah , Mathews Thomas P. , Chen Brandon , Zhao Zhiyu , Morrison Sean J. 
Published: October 2024

Abstract
Ascorbate (vitamin C) limits hematopoietic stem cell (HSC) function and suppresses leukemia development, partly by promoting the function of the Tet2 tumor suppressor. In humans, ascorbate is obtained from the diet, whereas in mice, it is synthesized in the liver. In this study, we show that deletion of the Slc23a2 ascorbate transporter from hematopoietic cells depleted ascorbate to undetectable levels in HSCs and multipotent hematopoietic progenitors (MPPs) without altering the plasma ascorbate levels. Slc23a2 deficiency increased HSC reconstituting potential and self-renewal potential upon transplantation into irradiated mice. Slc23a2 deficiency also increased the reconstituting and self-renewal potentials of MPPs, conferring the ability to reconstitute irradiated mice long term. Slc23a2-deficient HSCs and MPPs divided much less frequently than control HSCs and MPPs. Increased self-renewal and reconstituting potential were observed particularly in quiescent Slc23a2-deficient HSCs and MPPs. The effect of Slc23a2 deficiency on MPP self-renewal was not mediated by reduced Tet2 function. Ascorbate thus regulates quiescence and restricts self-renewal potential in HSCs and MPPs such that ascorbate deficiency confers MPPs with long-term self-renewal potential.
Lhx6 deficiency causes human embryonic palatal mesenchymal cell mitophagy dysfunction in cleft palate
Authors: Luo Haotian , Ieong Hio Cheng , Li Runze , Huang Delan , Chen Danying , Chen Xin , Guo Yuqing , Qing Yangqiao , Guo Bingyan , Li Ruoyu , Teng Yungshan , Li Wenfeng , Cao Yang , Zhou Chen , Wang Weicai 
Published: October 2024

Abstract
Background
Overconsumption of retinoic acid (RA) or its analogues/derivatives has been linked to severe craniomaxillofacial malformations, such as cleft palate and midface hypoplasia. It has been noted that RA disturbed the proliferation and migration of embryonic palatal mesenchymal (EPM) cells in these malformations, yet the exact mechanisms underlying these disruptions remained unclear.

Methods
A model of retinoic acid (RA)-induced cleft palate in fetal mice was successfully established. Histological alterations in the palate were evaluated using Hematoxylin and Eosin (H&E) staining and RNA in situ hybridization (RNAscope). Cellular proliferation levels were quantified via the Cell Counting Kit-8 (CCK-8) assay and EdU incorporation assay, while cell migration capabilities were investigated using wound healing and Transwell assays. Mitochondrial functions were assessed through Mito-Tracker fluorescence, mitochondrial reactive oxygen species (ROS) measurement, ATP level quantification, and mitochondrial DNA (mtDNA) copy number analysis. Differential gene expression and associated signaling pathways were identified through bioinformatics analysis. Alterations in the transcriptional and translational levels of Lhx6 and genes associated with mitophagy were quantified using quantitative PCR (qPCR) and Western blot analysis, respectively. Mitochondrial morphology and the mitochondrial autophagosomes within cells were examined through transmission electron microscopy (TEM).

Results
Abnormal palatal development in mice, along with impaired proliferation and migration of human embryonic palatal mesenchymal (HEPM) cells, was associated with RA affecting mitochondrial function and concomitant downregulation of Lhx6. Knockdown of Lhx6 in HEPM cells resulted in altered cell proliferation, migration, and mitochondrial function. Conversely, the aberrant mitochondrial function, proliferation, and migration observed in RA-induced HEPM cells were ameliorated by overexpression of Lhx6. Subsequent research demonstrated that Lhx6 ameliorated RA-induced dysfunction in HEPM cells by modulating PINK1/Parkin-mediated mitophagy, thereby activating the MAPK signaling pathways.

Conclusion
Lhx6 is essential for mitochondrial homeostasis via tuning PINK1/Parkin-mediated mitophagy and MAPK signaling pathways. Downregulation of Lhx6 by RA transcriptionally disturbs the mitochondrial homeostasis, which in turn leads to the proliferation and migration defect in HEPM cells, ultimately causing the cleft palate.

Graphical abstract
Peripheral blood to next-generation sequencing ready DNA library: a novel engineering design for automation
Authors: Naranbat Dulguunnaran , Brassard Lothar à , Lawandy Nabil , Tripathi Anubhav 
Published: October 2024

AbstractWhole genome sequencing (WGS) has become a gold standard for diagnosing genomic variation. Peripheral blood is a common sample source for the extraction of nucleic acids for Next-Generation Sequencing (NGS) applications. Here, we present an integrated and fully automated device design that uses new concepts of fluid mechanics, heat-mass transfer, and thermodynamics of enzymatic reactions to extract nucleic acids from the blood and perform DNA library preparation from a pre-filled plate. We demonstrate that the presented device effectively extracts dsDNA with an average of 25.03 µg/mL and 25.91 µg/mL yield from citrate-stabilized human peripheral blood stored in Fresh (4 °C) and Frozen (-20 °C) conditions, respectively. Furthermore, our method automatically extracts nucleic acids and creates a high-quality sequence-ready DNA library from blood stabilized with citrate and EDTA for 8 samples simultaneously in a single run with a total operation time of ~ 7 h. Our results show the required coverage and depth of the genome, highlighting an essential application of this device in processing blood samples for genome sequencing.
Graphical Abstract