bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probing the response of human osteoblasts following exposure to sympathetic neuron‐like PC‐12 cells in a 3D coculture model
Authors: Erndt‐Marino Josh D. , Hahn Mariah S. 
Published: November 2016

AbstractUnderstanding the capacity of the sympathetic nervous system (SNS) to regulate bone homeostasis has implications for a number of metabolic diseases and may help establish connections between certain neurological conditions and bone quality. The goal of the present work was to gain a deeper understanding of the influence of the SNS on the phenotype of osteoblasts, a major cell type in bone. An in vitro coculture model with human osteoblasts and sympathetic‐like, neuroendocrine pheochromocytoma‐12 (PC‐12) cells encapsulated within separate 3D poly(ethylene glycol) diacrylate (PEGDA) hydrogels was utilized to assess markers involved with bone ECM formation and osteoclast formation. In terms of bone ECM proteins, only osteopontin (OPN) was significantly increased in osteoblasts exposed to PC‐12 cells relative to osteoblast mono‐culture controls. In contrast, all bone resorption markers investigated (IL‐6, TNF, IL‐1β, VEGF‐A) were enhanced at the gene level and the ratio of osteoprotegerin (OPG) to RANKL was significantly decreased in osteoblasts exposed to PC‐12 cells. Cumulatively, these data indicate that the SNS may substantially influence bone resorption. Because of the context‐dependent nature of the SNS, future studies will characterize the secretion profile of neurotransmitters and neuropeptides from the PC‐12 cells in our model. Additionally, various SNS modulating pharmacologic agents will be examined for their capacity to reduce expression of bone resorption/inflammatory markers. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 984–990, 2017.
Protein Expression Differences of 2‐Dimensional and Progressive 3‐Dimensional Cell Cultures of Non‐Small‐Cell‐Lung‐Cancer Cell Line H460
Authors: Ravi Maddaly , Mohan Divya K. , Sahu Bellona 
Published: November 2016

ABSTRACTNon‐small‐cell‐lung‐cancer (NSCLC) constitutes about 75–80% of lung cancers. The challenge to tackle cancers is in early diagnosis and arriving at safer therapeutic options. In vitro studies using cancer cell lines continue to contribute significantly in understanding cancers. Cell culture methods have evolved and the recent developments in 3 dimensional (3D) cell cultures are inducing greater resemblance of the in vitro cultured cells with in vivo conditions. In this study, we established 3D aggregates of H460 cell line on agarose hydrogels and studied the protein expression differences among cells grown as monolayers (2D) and the progressively developing 3D aggregates from days 2 to 10. Analysis included matching of those proteins expressed by the developing aggregates and the available literature on progressing tumors in vivo. J. Cell. Biochem. 118: 1648–1652, 2017. © 2016 Wiley Periodicals, Inc.
Exposure‐Response Analyses Supporting Ticagrelor Dosing Recommendation in Patients With Prior Myocardial Infarction
Authors: Röshammar Daniel , Nyberg Joakim , Andersson Tomas , Stanski Donald , Storey Robert F. , Hamrén Bengt 
Published: November 2016

AbstractThe relationships between drug exposure and the composite risk of cardiovascular (CV) death, myocardial infarction (MI), and stroke as well as the risk of TIMI major bleeding were estimated following long‐term treatment with ticagrelor 60 or 90 mg twice daily in 20,942 patients with prior MI. These analyses support the primary reported efficacy and safety evaluations by showing that there were clear separations from placebo early in treatment with both doses, regardless of ticagrelor exposure, for both endpoints. In addition, the exposure‐response analyses provided new insight into the contribution of individual exposure levels, rather than dose, as a predictor of events and accounted for differences in the baseline risk between patients. The predicted risks of CV death/MI/stroke were similar despite an increase in the median predicted ticagrelor average steady‐state concentration from 606 nmol/L with ticagrelor 60 mg to 998 nmol/L with ticagrelor 90 mg (hazard ratios vs placebo of 0.83 and 0.81, respectively). The corresponding predicted risk of TIMI major bleeding slightly increased (hazard ratios vs placebo of 2.4 and 2.6, respectively). Apart from Japanese patients, showing a lower risk of CV death/MI/stroke, the response to ticagrelor was consistent across the study population, as supported by the combination of relatively flat exposure‐response relationships in the studied exposure range, similar sensitivity to ticagrelor exposure, and small exposure differences. Consequently, the present analyses support the selection of the 60‐mg dose for all demographic subgroups of patients studied.
An advanced analytical neuro–space mapping technique with sensitivity analysis for transistor modeling
Authors: Zhu Lin , Liu Kaihua , Liu Wenyuan , Zhang Qi‐jun , Wu Haifeng , Ma Yongtao , Peng Bo 
Published: November 2016

AbstractThis paper presents an advanced analytical neuro–space mapping (neuro‐SM) technique for accurate and efficient modeling of transistor devices. This is an improvement over the existing neuro‐SM, which aims to use neural networks to map a given approximate device model towards an accurate model. The proposed neuro‐SM retains the ability of the existing neuro‐SM in modifying the voltage relationship between the given approximate device model and the accurate model. The proposed technique can also map the current relationship between the given model and the accurate model. In this way, the proposed neuro‐SM can produce improved accuracy over the existing neuro‐SM. In addition, analytical formulas of mapping and sensitivities of the direct current, small‐signal S parameter, and large‐signal harmonic of the proposed neuro‐SM model with respect to mapping parameters and coarse‐model parameters are also derived. The sensitivity analysis can be used with a gradient‐based training technique to improve the model training efficiency. The validity and efficiency of the proposed approach are verified through 2 transistor modeling examples and use of the proposed neuro‐SM models in a large‐signal behavior analysis of an amplifier.
Study of the two‐dimensional sine‐Gordon equation arising in Josephson junctions using meshless finite point method
Authors: Kamranian Maryam , Dehghan Mehdi , Tatari Mehdi 
Published: November 2016

AbstractIn this paper, the finite point method is discussed for solving the initial‐boundary value problem associated with the sine‐Gordon equation in two‐dimensional domains arising in the Josephson junctions problem. The resulting nonlinear system is solved using an appropriate predictor‐corrector scheme. The proposed scheme is simple and efficient. The collisional properties for cases involving the most known from the bibliography, line, and ring solitons are studied in numerical results. Also the birth of a single Josephson vortex in a Josephson transmission line at a T‐shaped junction is studied.
Assessing seasonal variation of near surface air temperature lapse rate across India
Authors: Ojha Richa 
Published: November 2016

ABSTRACTPrecise characterization of surface air temperature at different spatial scales remains a challenge as it often requires a dense network of temperature sensors. The most common approach for estimating surface air temperature at a desired location in hydrological and terrestrial models is to interpolate surface air temperature observations obtained from a sparse set‐up of temperature sensors using the global standard uniform lapse rate. This interpolation technique, though easy to use, can lead to unreliable results, as the global standard uniform lapse rate may not be representative of seasonal and local variations in lapse rate. In this study, linear and nonlinear regression relationships are derived to estimate mean minimum, mean and mean maximum near surface air temperatures (at monthly and annual time scales) across India. The normal daily air temperature data from 439 stations and their corresponding latitude, longitude and elevation are used in the analysis. The computed temperature gradients exhibit seasonal variation. The effects of elevation and longitude are more pronounced in pre‐monsoon (March–May) and post‐monsoon (October–November) months, whereas the effects of latitude are more in winter months (December–February). Results indicate that the global standard uniform lapse rate (− 0.65 ° C 100 m− 1) is only applicable for monthly mean maximum temperature for April and May. The highest interpolation reliability is obtained for monthly mean minimum temperature. The regression equations though simple, hold promise for describing surface air temperature across India.
Nomogram for predicting central node metastasis in papillary thyroid carcinoma
Authors: Kim Seo Ki , Chai Young Jun , Park Inhye , Woo Jung‐Woo , Lee Jun Ho , Lee Kyu Eun , Choe Jun‐Ho , Kim Jung‐Han , Kim Jee Soo 
Published: November 2016

BackgroundThere was a difficulty for detecting Central lymph node metastasis (CLNM) in papillary thyroid carcinoma (PTC) patients. Therefore, the purpose of this study was to design a nomogram for predicting CLNM.MethodsA total of 10,763 PTC patients who underwent total thyroidectomy with central neck dissection (CND) in Samsung Medical Center were randomly assigned to the training set (n = 7,535) and to the internal validation set (n = 3,228). And, a total of 2,514 PTC patients who underwent total thyroidectomy with CND at Seoul National University Hospital were assigned to the external validation set.ResultsThe values of the area under the receiver operating characteristic curve in the training set, internal validation set, and external validation set were 0.721 (95% confidence interval [CI], 0.709–0.732), 0.706 (95%CI, 0.688–0.724), and 0.706 (95%CI, 0.685–0.727), respectively.ConclusionsWe recommend the use of our nomogram to enable clinicians and patients to easily personalize and quantify the probability of CLNM during the both pre‐ and postoperative period. Clinicians may consider the prophylactic CND and meticulous postoperative evaluation in PTC patients with a high nomogram score. J. Surg. Oncol. 2017;115:266–272. © 2016 Wiley Periodicals, Inc.
Mediterranean diet–gene interactions: A targeted metabolomics study in Greek‐Cypriot women
Authors: Kakkoura Maria G. , Sokratous Kleitos , Demetriou Christiana A. , Loizidou Maria A. , Loucaides Giorgos , Kakouri Eleni , Hadjisavvas Andreas , Kyriacou Kyriacos 
Published: November 2016

ScopeA high adherence to the Mediterranean diet (MD) was previously associated with a decreased risk of breast cancer (BC) among Greek‐Cypriot women. Additionally, particular polymorphisms were shown to modulate this MD‐BC association. Herein, we aimed to investigate the effect of polymorphisms–MD interactions on the levels of specific metabolites that could be related to dietary adherence or enzymatic activity, which is itself modulated by polymorphisms.Methods and resultsGreek‐Cypriot women who were BC controls and had the lowest or the highest MD adherence (vegetables, fruit, legumes, fish) as assessed by principal component analysis (n = 564) were included. Participants were previously genotyped for nine polymorphisms of the one‐carbon metabolism, oxidative stress, and xenobiotic metabolism. The serum levels of 14 metabolites that are key players in the aforementioned pathways were measured by UPLC‐MS/MS. ANCOVA was used to assess polymorphism–MD interactions on metabolites’ levels within a multivariate linear regression model. Statistically significant interactions between GSTM1 (where GST is glutathione S‐transferase) deletion polymorphism and MD on flavin mononucleotide and on 5‐methyltetrahydrofolate (5‐MTHF) concentrations were observed. The MTHFR rs1801133 interacted significantly with MD on 5‐MTHF concentration.ConclusionSerum levels of flavin mononucleotide and 5‐MTHF were shown to be influenced by interactions between GSTM1 deletion or MTHFR (rs1801133) polymorphisms and a dietary pattern, characteristic of MD.
A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane‐treated colon cancer cells
Authors: Johnson Gavin S. , Li Jia , Beaver Laura M. , Dashwood W. Mohaiza , Sun Deqiang , Rajendran Praveen , Williams David E. , Ho Emily , Dashwood Roderick H. 
Published: November 2016

ScopeThe anticancer agent sulforaphane (SFN) acts via multiple mechanisms to modulate gene expression, including the induction of nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2)‐dependent signaling and the inhibition of histone deacetylase activity. Transcriptomics studies were performed in SFN‐treated human colon cancer cells and in nontransformed colonic epithelial cells in order to pursue new mechanistic leads.Methods and resultsRNA‐sequencing corroborated the expected changes in cancer‐related pathways after SFN treatment. In addition to NAD(P)H quinone dehydrogenase 1 (NQO1) and other well‐known Nrf2‐dependent targets, SFN strongly induced the expression of Loc344887. This noncoding RNA was confirmed as a novel functional pseudogene for NmrA‐like redox sensor 1, and was given the name NmrA‐like redox sensor 2 pseudogene (NMRAL2P). Chromatin immunoprecipitation experiments corroborated the presence of Nrf2 interactions on the NMRAL2P genomic region, and interestingly, NMRAL2P also served as a coregulator of NQO1 in human colon cancer cells. Silencing of NMRAL2P via CRISPR/Cas9 genome‐editing protected against SFN‐mediated inhibition of cancer cell growth, colony formation, and migration.ConclusionNMRAL2P is the first functional pseudogene to be identified both as a direct transcriptional target of Nrf2, and as a downstream regulator of Nrf2‐dependent NQO1 induction. Further studies are warranted on NMRAL2P‐Nrf2 crosstalk and the associated mechanisms of gene regulation.
Analytical prediction of aggregates' effects on the ITZ volume fraction and Young's modulus of concrete
Authors: Zouaoui R. , Miled K. , Limam O. , Beddey A. 
Published: November 2016

SummaryIn order to evaluate analytically the ITZ volume fraction (fITZ) in concrete, a three phase model is proposed for the random concrete microstructure using the Voronoï tessellation. Within this model, the ITZ local thickness is a statistical variable depending on the local paste thickness available between each couple of neighbouring aggregates. The fITZ is found to not exceed 7% for typical concretes. Then, the concrete Young's modulus is predicted analytically using a four‐phase generalized self consistent model but in which the proposed fITZ is considered. It is found that the concrete Young's modulus increases when increasing aggregates volume fraction, aggregates maximum size and the proportion of coarse aggregates and when decreasing the ITZ thickness and Young's modulus. Finally, the validity of the proposed model is discussed based on a comparison between its predictions and three sets of experimental results related to normal and high strength concretes taken from literature. Copyright © 2016 John Wiley & Sons, Ltd.
Avoidant conversations about death by clinicians cause delays in reporting of neutropenic sepsis: Grounded theory study
Authors: Oakley Catherine , Taylor Cath , Ream Emma , Metcalfe Alison 
Published: November 2016

AbstractBackgroundEvidence suggests that patients delay reporting symptoms of neutropenic sepsis (NS) despite the risk to their life. This study aimed to elicit factors that contribute to delayed patient reporting of NS symptoms.MethodsA constructivist grounded theory study used observations of chemotherapy consultations (13 h) and 31 in‐depth interviews to explore beliefs, experiences, and behaviors related to NS. Participants included women with breast cancer, their carers (partners, family, or friends), and clinicians. An explanation for patient delays was developed through theoretical sampling of participants to explore emerging areas of interest and through constant comparison of data and their coding. This entailed iterative and concurrent data collection and analysis. Data were collected until saturation.ResultsAll patients who developed NS‐type symptoms delayed presenting to hospital (2.5 h‐8 days), sometimes repeatedly. Moderators of delay included metastatic disease, bereavement, fatalism, religious beliefs, and quality of relationships with clinicians. There was an interplay of behaviors between clinicians, patients, and carers where they subconsciously conspired to underplay the seriousness and possibility of NS occurring.ConclusionsFindings have implications for health risk communication and development of holistic service models.
Distributed event‐triggered feedback consensus control with state‐dependent threshold for general linear multi‐agent systems
Authors: Liu Xiangdong , Du Changkun , Lu Pingli , Yang Dapeng 
Published: November 2016

SummaryThis paper considers the distributed event‐triggered consensus problem for multi‐agent systems with general linear dynamics under undirected graphs. Based on state feedback, we propose a novel distributed event‐triggered consensus controller with state‐dependent threshold for each agent to achieve consensus, without continuous communication in either controller update or triggering condition monitoring. Each agent only needs to monitor its own state continuously to determine if the event is triggered. It is proved that there is no Zeno behavior under the proposed consensus control algorithm. To relax the requirement of the state measurement of each agent, we further propose a novel distributed observer‐based event‐triggered consensus controller to solve the consensus problem in the case with output feedback and prove that there is no Zeno behavior exhibited. Finally, simulation results are given to illustrate the theoretical results. Copyright © 2016 John Wiley & Sons, Ltd.
Postcapture robust nonlinear control for tethered space robot with constraints on actuator and velocity of space tether
Authors: Huang Panfeng , Wang Dongke , Zhang Fan , Meng Zhongjie , Liu Zhengxiong 
Published: November 2016

SummaryStabilization control is an essential mission for the tethered space robot‐target combination during the postcapture phase of tethered space robot (TSR). In this paper, the stabilization problem of such a tumbling combination is studied. With the consideration of the space tether and the attitude of the TSR's gripper, the dynamic model of the combination is first derived using Lagrange method. Then a robust nonlinear controller for the combination is proposed based on the backstepping control method. Considering the constraint on the velocity of the space tether, command filter method is utilized to guarantee the velocity of the space tether within a permitted range. A feedback term is designed to compensate the saturation of the thruster. Moreover, an adaptive law is designed to estimate the disturbance of parameter uncertainties and this disturbance is compensated in the proposed controller. Numerical simulations suggest that the proposed robust controller can realize the orbit and attitude stabilization of the combination; besides, the velocity of the space tether is effectively constrained and the parameter uncertainties of the combination can be compensated via the adaptive law. Copyright © 2016 John Wiley & Sons, Ltd.
Adaptive block‐based pixel value differencing steganography
Authors: Hosam Osama , Ben Halima Nadhir 
Published: November 2016

AbstractSteganography is the science of hiding secure data in digital carriers such as images and videos. Pixel value differencing (PVD) steganography algorithms embed data into images depending on pixel neighborhood differences. We have proposed PVD scheme for embedding secure data into digital images. The image is divided into non‐overlapping 3 × 3 blocks. The block's median pixel is used as a reference for calculating pixel differences. The distance between the minimum and maximum differences are fine tuned for spreading the secure data on a wide range of image regions with high‐intensity fluctuations. The embedding procedure embeds secure data into the content regions with edges and intensity transitions. Texture images provide higher embedding size compared with regular images. The results showed that the proposed algorithm is successfully able to avoid smooth regions in the embedding process. In addition, the proposed algorithm shows better embedding quality compared with the state of the art PVD approaches especially with low‐embedding rates. Copyright © 2016 John Wiley & Sons, Ltd.
Towards a complete chaotic map‐based mobile RFID authentication protocol with efficient anticollision tag indexing
Authors: Benssalah Mustapha , Djeddou Mustapha , Drouiche Karim 
Published: November 2016

AbstractRadio frequency identification technology is one of the modern popular emerging technologies proven to advance a wide range of applications in both academia and industry. However, potential security risks, privacy issues, and efficiency are still open challenges that must be addressed seriously to achieve enhanced protection. In this paper, we show that the Akgün et al. protocol remains unsafe and imperfect in terms of the security issue and database identification efficiency. We, first, demonstrate that the Akgün et al. protocol suffers from a stolen/lost reader attack, which could expose users to some life threatening situations and unwanted collection of sensitive private information. Second, we found that all indexing identification‐based protocols and exceptionally the Akgün et al. protocol cannot be used properly when the collision of tag index occurs, which exhibits a low efficiency in the database entry identification process. As the Akgün et al. protocol is almost perfect, it is worthy to be enhanced. Therefore, towards a complete and secure mobile radio frequency identification authentication protocol, we propose efficient solutions that could overcome the aforementioned security problem and offer high identification efficiency using efficient anticollision procedure based on dynamic linked list data structure principle. Via informal and formal security analysis, we show that the improved version is insensitive to the most common attacks and is more efficient than the existing schemes in the literature. Copyright © 2016 John Wiley & Sons, Ltd.
Lateral drift constrained structural optimization of an actual supertall building acted by wind load
Authors: Xu An , Sun Wei‐Xing , Zhao Ruo‐Hong , Wu Jiu‐Rong , Ying Wei‐Qin 
Published: November 2016

AbstractRecent trends towards constructing taller and increasingly slender buildings imply that these structures are more sensitive to wind excitation. This paper presents a technique for the wind‐resistant optimal design of supertall buildings with a complex structural system including concrete‐filled steel tube columns, shear walls, and various types of beams and columns. In each optimal design cycle, the dynamic wind load acting on a building is transformed into a set of multiple‐oriented equivalent static wind loads, which converts the optimal design for a building acted by dynamic loads into a simpler optimal design problem that considers only static loads. The objective function and constraint functions are explicitly formulated for various types of frame and area members, and consequently, the optimal design problem is mathematically modeled. The optimality criteria method is employed to seek a solution to the optimal design problem. A 68‐story actual supertall building with a height of 303 m is considered for a case study. The obtained results show that the presented technique is capable of giving a good numerical optimal solution for practical use. The technique and results obtained from this study are valuable for academic and professional engineers involved in wind engineering and structural design.