bluuebunny/crossref_metadata_2025_split huggingface dataset:
FORMULASI PANGAN DARURAT BERBENTUK FOOD BARS BERBASIS TEPUNG MILLET PUTIH (Panicum milliaceum L.) DAN TEPUNG KACANG MERAH (Phaseolus vulgaris L.)
Authors: Katri Anandito Raden Baskara , Siswanti Siswanti , Nurhartadi Edhi , Hapsari Rini 
Published: November 2016

This study aimed to obtain a formula emergency food in the form food bars made from white millet flour and red bean flour. Foodbars made with Intermediate Moisture Food (IMF) technology with wet dyeing technique. This study used completely randomized design (CRD), which consists of a single factor, namely the variation formula white millet flour and red bean flour. The results showed that the formula food bars with the highest level of consumer acceptance in the composition of 15 g of white millet flour, red bean flour 10 g, 2 g sugar, 10 g margarine, milk full cream 13 g, 6.043 g and the addition of water. In 100 g of food bars contained water, ash, protein, fat, carbohydrate, and caloric value a respectively of 16.45%, 1.45%, 10.99%, 35.39%, 42.26%, 0 , 81 and 233.80 kcallbar.Keywords: Emergency food, food bars, red bean flour, white millet flour ABSTRAKPenelitian ini bertujuan untuk memperoleh formula pangan darurat berbentuk food bars berbahan dasar tepung millet putih dan tepung kacang merah. Food bars dibuat dengan teknologi Intermediate Moisture Food (IMF) dengan teknik pencelupan basah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari satu faktor yaitu variasi formula tepung millet putih dan tepung kacang merah (15:10; 12,5:12,5; 10:15). Hasil penelitian menunjukkan bahwa formula food bars dengan tingkat penerimaan konsumen tertinggi pada komposisi tepung millet putih 15 g, tepung kacang merah 10 g, gula halus 2 g, margarine 10 g, susu full cream 13 g, dan penambahan air 6,043 g. Dalam 100 g food bars terkandung air, abu, protein, lemak, karbohidrat nilai a dan kalori berturut-turut sebesar 16,45%,1,45%, 10,99%, 35,39%, 42,26%, 0,81 dan 233,80 kkallbar.Kata kunci: Food bars, pangan darurat, tepung kacang merah, tepung millet putih
OPTIMASI MEDIA FERMENTASI Aspergillus oryzae, PENGHASIL ANTIJAMUR PATOGEN BUAH KAKAO Phytophthora palmivora Fermentation Medium Optimization of Aspergillus oryzae, Antifungals Producer for Cacao Pathogen Phytophthora palmivora
Authors: Rahma Rizka Aulia , Widjanarko Simon Bambang , Sunaryanto Rofiq , Yunianta Yunianta 
Published: November 2016

Indonesia was known as a producer and exporter of cacao, but nowadays Indonesia had an issue in cacao production caused by microorganisms (Phytophtora palmivora) that capable of reducing the amount of cacao production each year. This study follows up a previous study that had found compounds that are actively against at Phytophtora palmivora from Aspergillus oryzae metabolites. In this study the compound will be produced in greater numbers to optimize fermentation medium. The first stage was conducted to determine the profile of the active compound and to analyze the best carbon, nitrogen source for Aspergillus oryzae fermentation medium. The second stage was designed by using Response Surface Method (RSM) with Central Composite Design (CCD) to optimize two variables in the fermentation medium, glucose concentration (the best carbon source) and the concentration of peptone (best nitrogen source). The expected response was the most active compounds percentage of the area. Data analysis was performed by Design Expert DX 7.1.5 program. The first stage of this study presumed that Tenuazonic acid compounds with a molecular weight of 198.1126 gram.mol was an antifungal compound produced by Aspergillus oryzae fungus to prevent Phythopthora palmivora fungus pathogens cacao pods. In addition the results are that glucose was the best carbon source (mean % relative area of antifungal compounds: 85.39%) and that peptone was selected as the best source of nitrogen (mean % relative area of antifungal compounds: 91.07%). The second stage showed the optimum conditions of fermentation medium antifungal Aspergillus oryzae was the composition of glucose 35.25 g/L and peptone 16.7 g/L. The combination of both compositions would yield a mean response of laboratory data % relative area of antifungal compounds 91.2217% and the estimated value (prediction) by Design Expert models 92.2936%. Difference in the estimated values (the response of the model) to the value of the results of response in laboratory experiments at 1.1614%, then it could be concluded that the model used was appropriate and able to explain the applied experimentaldata.-1Kata kunci: Cacao, Aspergillus oryzae, Phytophtora palmivora, active compound, optimization of fermentation medium, Response Surface Method (RSM)
REDUKSI PEMBOROSAN UNTUK PERBAIKAN VALUE STREAM PRODUKSI “MI LETHEK” MENGGUNAKAN PENDEKATAN LEAN MANUFACTURING (Waste Reduction to Improve Value Stream of “Mi Lethek” Production Using Lean Manufacturing Approach)
Authors: Nugroho Aditya , Ainuri Makhmudun , Khuriyari Nafis 
Published: November 2016

“Mi Lethek” industry is an industry that produce dry noodles. In the production process of “Mi Lethek” industry, unnecessary inventory and excessive transportation. To reduce that waste, lean manufacturing approach is required. industry was 12,05%. The recommendations for increase PCE are relayouting the plant and change the order schedulingof raw materials. These recommendations could increase PCE score to 15,68 %.Keywords: Waste, value stream, “Mi Lethek”, lean manufacturing ABSTRAK Industri “Mi Lethek” merupakan industri yang menghasilkan produk berupa mi kering berbahan baku tepung tapioka. Pada proses pengolahan mi di industri “Mi Lethek”, terdapat berbagai pemborosan () yang dapat merugikan industri. Diantara pemborosan yang terjadi berupa persediaan bahan baku yang belum diperlukan dan transportasi berlebih. Untuk mereduksi pemborosan tersebut diperlukan suatu perbaikan pada menggunakan pendekatan . Pendekatan aktivitas yang ada pada industri “Mi Lethek”. Aktivitas-aktivitas tersebut kemudian digolongkan menjadi dua jenis aktivitas, yaitu aktivitas yang memberikan nilai tambah dan aktivitas yang tidak memberikan nilai tambah. Waktu dari masing-masing aktivitas tersebut yang selanjutnya digunakan untuk menghitung nilai (PCE). nilai pada produk dibandingkan total waktu yang digunakan produk selama dalam proses. Berdasarkan penelitian yang telah dilakukan, didapatkan nilai PCE awal dari industri “Mi Lethek” sebesar 12,05 %Perbaikan yang dilakukan ialah dengan mengubah tata letak pabrik dan melakukan perbaikan penjadwalan pemesanan bahan baku. Hasil perbaikan tersebut berhasil meningkatkan nilai PCE menjadi 15,68 %.Kata kunci: Pemborosan, “Mi Lethek”
PENGARUH PERBEDAAN WARNA PIGMEN BERAS ORGANIK TERHADAP AKTIVITAS ANTIOKSIDAN
Authors: Sri Widyawati Paini , Maya Suteja Anita , Putut Suseno Thomas Indarto , Monica Pricilia , Saputrajaya William , Liguori Christian 
Published: November 2016

Organic Rices, such as Jasmine variety white organic rice, Saodah variety red organic rice, and Java variety black organic rice, are many cultivated by farmer in Indonesia, especially Sleman area, DI Yogyakarta. The potency of three varieties of organic rice as antioxidant source hasn’t been studied. Therefore this research was done to know the effect of pigment color difference in three varieties of organic rice on antioxidant activity, especially 1,1-diphenyl-2- pycrylhydrazyl (DPPH) radical scavenging activity and ion reducing power. The results showed that Saodah variety red organic rice had the highest antioxidant potency because it had the highest total phenol and total flavonoid, 37.93 mg GAE/g sample dry base and 0.85 mg CE/g sample dry base, respectively. However Saodah variety red organic rice had total anthocyanin lower than that of Java variety black organic rice, 0.0025 mg/g sample dry base and 0.024 mg/g sample dry base, respectively. Anthocyanin compounds identified in three varieties of organic rice were cyanidine-3-glucoside at 32 minute time retention and peonidine -3-glucoside at 37 minute time retention. 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging activity and ion reducing power of Saodah variety red organic rice were the highest, 0.90 mg equivalent vitamin E/g sample dry base and 278.28 mg equivalent vitamin E/g sample dry base, respectively.Keywords: Pigment, organic rice, antioxidant activity ABSTRAKBeras organik, meliputi beras putih varietas Jasmine, merah varietas Saodah, dan hitam varietas Jawa, banyak dibudidayakan oleh masyarakat di Indonesia, terutama daerah Sleman, D.I. Yogyakarta. Potensi ketiga jenis beras tersebut sebagai sumber antioksidan belum banyak dikaji. Oleh karena itu penelitian ini dilakukan untuk mengetahui pengaruh perbedaan pigmen pada ketiga jenis beras organik tersebut terhadap aktivitas antioksidan, khususnya kemampuan menangkap radikal bebas 2,2-difenil-1-pikrilhidrasil (DPPH) dan mereduksi ion besi. Hasil menunjukkan bahwa beras organik merah varietas Saodah paling berpotensi sebagai sumber antioksidan. Hal ini ditunjukkan dengan total fenol dan total flavonoid beras merah tertinggi dibandingkan kedua beras yang lain, masing-masing sebesar 37,93 mg GAE/g sampel db dan 0,85 mg CE/g sampel db. Meskipun beras organik merah mempunyai total antosianin (0,0025 mg/g sampel db) lebih rendah dari beras organik hitam (0,024 mg/g sampel db). Jenis senyawa antosianin yang terdeteksi pada ketiga jenis organik ini adalah sianidin-3-glukosida yang terdeteksi pada waktu retensi 32 menit dan peonidin-3-glukosida dengan waktu retensi 37 menit. Kemampuan menangkap radikal bebas DPPH dan kemampuan mereduksi ion besi beras organik merah tertinggi, masing-masing sebesar 0,90 mg ekuivalen vit E/g sampel db dan278,28 mg ekuivalen vit E/g sampel db.Kata kunci: Pigmen, beras organik, aktivitas antioksidan
SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus)
Authors: Widowati Tri Wardani , Hamzah Basuni , Wijaya Agus , Pambayun Rindit 
Published: November 2016

Lactobacillus sp B441 and II442 were species of lactic acid bacteria isolated from tempoyak, a kind of food fermented made from durian flesh. Staphylococcus aureus is a pathogenic bacteria that has natural habitat on the surface of human body such as on the skin and in mucous membranes like the nose. Contamination of Staphylococcus aureuswas a risk factor in tempoyak fermentation, especially, during taking the durian flesh from seeds. The aim of this research was to observe antagonistic property of Lactobacillus sp B441 and II442 isolated from tempoyak during low temperature fermentation against Staphylococcus aureus. Phenotypic identification using API 50 CHL test kit indicatedthat isolates were Lactobacullus plantarum B441 and Lactobacillus plantarum II442. Based on co-culture analysis show that isolates of Lactobacillus plantarum B441 and II442 have bactericidal effect against Staphylococcus aureus ATCC 25923 as a indicator of pathogenic bacteria. At 25 th hours, population of Staphylococcus aureus ATCC 25923 in mix-culture with Lactobacillus plantarum B441 and Lactobacillus plantarum II442 decreased 3 – 4 log cycle from initial population. However, population of Staphylococcus aureus ATCC 25923 in mono-culture increased 2 log cycles at 25 th hours. Keywords: Lactobacillus sp, tempoyak, antagonism, Staphylococcus aureus ABSTRAKLactobacillus sp B441 dan II442 merupakan spesies bakteri asam laktat yang diisolasi dari tempoyak yaitu makanan fermentasi dari daging buah durian. Staphylococcus aureus adalah bakteri patogenik yang habitat alaminya pada permukaan kulit manusia dan membran mukosa seperti hidung. Proses fermentasi tempoyak khususnya saat pemisahandaging buah durian dari bijinya beresiko terkontaminasi oleh Staphylococcus aureus. Tujuan dari penelitian ini adalah mengkaji sifat antagonistik dari Lactobacillus sp B441 and II442 yang diisolasi dari tempoyak terhadap Staphylococcus aureus. Identifikasi secara fenotif menggunakan API 50 CHL test kit menunjukkan bahwa isolat bakteri asam laktat tersebut adalah Lactobacullus plantarum B441 dan Lactobacillus plantarum II442. Berdasar uji co-culture diketahuibahwa kedua spesies tersebut mempunyai efek bakterisidal terhadap bakteri Staphylococcus aureus ATCC 25923. Pada jam ke 25 Staphylococcus aureus yang ditumbuhkan secara mix-culture dengan Lactobacillus plantarum B441 dan Lactobacillus plantarum II442 asal tempoyak turun 3 – 4 log cycle dari populasi awal. Sedangkan populasiStaphylococcus aureus ATCC 25923 secara mono-culture meningkat sekitar 2 log cycle pada jam ke 25.Kata kunci: Lactobacillus sp, tempoyak, antaginistik, Staphylococcus aureus
KAJIAN TEKNIS-EKONOMIS ALAT PENGERING PATI SAGU MODEL CROSS FLOW VIBRO FLUIDIZED BED (Study on Technical-Economic of Sago Starch Dryer Model of Cross Flow Vibro Fluidized Bed)
Authors: Jading Abadi , Payung Paulus , Reniana Reniana 
Published: November 2016

The purpose of this research was to evaluate performance of vibro cross flow fluidized bed dryer using biomass fuel for drying sago starch. The phase of research were evaluation of dryer heated by a biomass stove using coconut shell as a fuel and observation of the drying temperature, moisture content, drying time, energy analysis, and drying effiviency, as well as calculation of its economic analysis. The dryer has dimension of 200x50x1500 cm for length, width, hight, respectively, and working capacity of 35kg/process. The results showed that biomass fuel consumption for drying sago starch for 7 hours was 12740,00 MJ (70 kg/process) and electricity consumption for blower and vibrator was 37,80 MJ. Furthermore, the dryer reduced moisture content of sago starch from 42% (wb) to 12% (wb) with temperature in the vibrator chamber of 40-60 oC and relative humidity of 50%, as well as ambient temperature of 30 3C and drying efficiency of 46,02%. Economic analysis showed that the dryer had NPV of Rp. 16.002.858, BCR of 1,53, IRR of 35%, and PBP of 3,51 years.Keywords: Cross flow, vibro fluidized bed dryer, biomass stoves, sago starch, financial analysis ABSTRAKTujuan penelitian ini adalah melakukan pengujian terhadap alat pengering pati berbasis sagu model cross flow vibrofluidized bed bertenaga biomassa. Tahapan penelitian meliputi pengujian alat pengering menggunakan tungku biomassa berbahan bakar tempurung kelapa dengan melakukan pengamatan suhu selama pengeringan, penurunan kadar air, waktu pengeringan, kebutuhan energi, efisiensi pengering, dan analisis finansial. Konstruksi alat pengering pati sagu model vibro fluidized bed bertenaga biomassa berukuran panjang, lebar dan tinggi masing-masing (200x50x1500) cm, serta memiliki daya tampung atau kapasitas maksimum pati sagu basah 35 kg/proses. Hasil pengujian menunjukkan bahwa alat pengering ini mampu mengeringkan pati sagu selama 7 jam, dengan konsumsi bahan bakar tempurung kelapa sebanyak 70 kg/proses (1274MJ), kebutuhan daya listrik untuk tenaga blower dan vibrator(37,80 MJ), serta mampu menurunkan kadar air pati sagu dari 42%bb menjadi 12%bb dengan suhu dalam ruang vibrator adalah 40-60 oC, RH 50%, suhu lingkungan 30C dan efisiensi pemanasan (pengeringan) 46,02%.Hasil analisis finansial menunjukan bahwa investasi alat pengering vibro fluidized bedsangat layak dilaksanakan berdasarkan kriteria NPV = Rp. 16.002.858, BCR= 1,53, IRR= 35%, dan PBP tertutupi setelah 3,51 tahun. Kata kunci: Aliran silang, vibro fluidized bed dryer, tungku biomassa, pati sagu, analisis finansial
OPTIMASI FERMENTASI BAGAS TEBU OLEH Zymomonas mobilis CP4 (NRRL B-14023) UNTUK PRODUKSI BIOETANOL (Optimization of Sugarcane Bagasse Fermentation by Zymomonas mobilis CP4 (NRRL B-14023) for Bioethanol Production)
Authors: Ernes Atmiral , Ratnawati Lia , Wardani Agustin Krisna , Kusnadi Joni 
Published: November 2016

Second generation bioethanol can be produced from fermentation of natural renewable materials, such as agricultural crops, as well as from industrial and domestic waste. The present study was aimed to optimize the fermentation process (inoculum concentration, urea concentration, and fermentation time) for ethanol production from sugarcane bagasse byZymomonas mobilis CP4 using response surface methodology (RSM) central composite experimental design (CCD). The RSM model predicted the optimum value of ethanol content was 1.257% (v/v) at inoculum concentration 15% (v/v), urea concentration 0.3% (w/v), and fermentation time 45 h. Based on the experiment, the ethanol concentrationwas 1.213% (v/v), which was in close agreement with the predicted value. Ethanol yield of this experiment was 0.479 with fermentation efﬁ ciency of 93.9%. The results presented here proved a signiﬁ cant contribution of Z. mobilis CP4 to the production of bioethanol from sugarcane bagasse.Keywords: Bioethanol, sugarcane bagasse, Zymomonas mobilis CP4, fermentation optimization ABSTRAKBioetanol generasi kedua dapat diproduksi dari fermentasi bahan terbarukan, seperti produk hasil pertanian, dan limbah atau hasil samping pengolahan industri dan rumah tangga. Tujuan penelitian ini adalah optimasi parameter fermentasi yang meliputi konsentrasi inokulum, konsentrasi urea, dan lama fermentasi untuk produksi etanol dari bagas tebu oleh Zymomonas mobilis CP4 dengan menggunakan response surface methodology (RSM) central composite experimental design (CCD). Kondisi respon yang optimal berdasarkan prediksi model diperoleh pada konsentrasi inokulum 15% (v/v), konsentrasi urea 0,3% (b/v), dan lama fermentasi 45 jam, dengan prediksi respon kadar etanol sebesar 1,257%(v/v). Berdasarkan hasil penelitian, kadar etanol optimal diperoleh sebesar 1,213% (v/v), yang menunjukkan hasil yang tidak berbeda jauh dengan prediksi model. Yield etanol yang diperoleh sebesar 0,479 dengan eﬁ siensi fermentasi 93,9%. Hasil penelitian ini membuktikan bahwa strain bakteri Zymomonas mobilis CP4 memiliki potensi yang cukup menjanjikan sebagai mikroba penghasil etanol.Kata kunci: Bioetanol, bagas tebu, Zymomonas mobilis CP4, optimasi fermentasi
Morphometric characteristics of TGF-β1-positive cells of fetal rat brain in vitro
Authors: Liubich L. , Lisyany M. , Malysheva T. , Semenova V. , Staino L. , Vaslovich V. 
Published: November 2016

One of the directions of cell therapy being developed for brain gliomas is the use of the neurogenic stem and progenitor cells (NSCs/NPCs). There are data on the anti-tumor and immunomodulating properties of the NSCs/NPCs the mechanisms of which were not disclosed yet. One of the potential targets for tumor therapy is the transforming growth factor β (TGF-β1) which is thought to be one of the key molecules in the regulation of proliferation, differentiation and cell survival or apoptosis. In the view of available information about the possibility of TGF-β1 production by the mammalian multipotent NSCs/NPCs, the aim of this work was to study the TGF-β1-positive cells in the dynamics of cultivation of fetal brain neurogenic cells as a potential source of anti-tumor or immunomodulating effects of these cells.Material and methods. The fetal rat brain cells on 14th (E14) day of gestation were used as the source for cultivation in standard conditions (DМЕМ + 1 % fetal bovine serum) and studied on the 2nd and 37thday by morphometry and immunocytochemistry.Results. In the fetal rat brain cell cultures, the TGF-β1-positive cells made 22.04 ± 2.33 % and the nestin-positive cells made 49.16 ± 10.60 % of the total cells number. The morphometric parameters of TGF-β1-positive cells exceeded the corresponding values of negative cells (average values of cross-sectional areas of the cytoplasm, cross-sectional areas of the nucleus, nuclear-cytoplasmic ratio). During cultivation the relative amount of TGF-β1-positive cells was slightly decreased 15.27 ± 9.80 % (p = 0.7) and their sizes were increased. On the 37th day of cultivation the sizes of TGF-β1-positive and their nuclei were smaller in the comparison with the TGF-β1-negative cells.Conclusions. The presence of TGF-β1 expression by part of neurogenic cells of fetal rat brain (E14) in vitro was found, which persisted throughout cultivation (~5 weeks). Significant quantitative differences of morphometric parameters of TGF-β1-positive and negative cells were detected.
Potentiation of directed osteogenic differentiation of thymic multipotent stromal cells by prior co-cultivation with thymocytes
Authors: Nikolskiy I. , Nikolskaya V. , Demchenko D. , Zubov D. 
Published: November 2016

It is known that multipotent stromal cells (MSCs) and thymocytes possess membrane affinity and interaction in the thymic niches that is essentially important for thymocytes differentiation. However there are no data about possible influence of intercellular contacts in the reverse direction: from the thymocytes to the MSCs.Materials and methods. The MSCs were obtained from the thymuses of С57ВL mice, using the explants technique, and cultivated under standard conditions during 8-12 passages. Thymocytes or bone marrow cells (106) were added to 4×104 MSCs for 24 hours. Thereafter they were eliminated and standard culture medium was changed by osteogenic or adipogenic differentiation medium and cultured during 10 days. After fixation the cells were stained by 1 % alizarin red S solution or 0.2 % solution of oil red О respectively. After extraction of the stains with 10 % acetic acid or isopropyl alcohol the optic density of extracts at 520 nm was measured.Results. We found that thymic multipotent stromal cells of the C57BL mice were effectively differentiated in vitro into the osteogenic and adipogenic lineages in the appropriate differentiation media that was evidenced by alizarin red and oil red staining of cell cultures. According to the results of measurement of optic density of the dye extracts, it was found that effectiveness of thymic MSCs differentiation into the osteogenic lineage after prior short-term co-cultivation with the thymocytes is increased.Conclusions. The contact of thymic stromal cells with thymocytes but not with bone marrow cells in the previous 24 hours potentiates the osteogenic differentiation and has no effect on the adipogenic cells maturation.
A novel model of periventricular leukomalacia on mouse organotypic brain slice culture
Authors: Tsupykov O. , Lushnikova I. , Nikandrova Y. , Yatsenko K. , Ustymenko A. , Kyryk V. , Butenko G. , Skybo G. 
Published: November 2016

The creation of adequate in vitro and in vivo models of neural tissue injury is essential to assess the therapeutic effect of pharmacological agents and regenerative potential of various types of stem cells in diseases of the central nervous system. The aim of this work was to create a novel model of cerebral white matter lesions – periventricular leukomalacia (PVL) – on murine organotypic brain slice culture.Materials and methods. The PVL model was developed on cultured organotypic mice brain slices subjected to oxygen-glucose deprivation (OGD) followed by addition of endotoxin lipopolysaccharide (LPS) in the culture medium. To analyze the degree of tissue injury within PVL simulation, we used spectrophotometric method for estimation of cytosolic enzyme lactate dehydrogenase (LDH) in the culture medium and immunohistochemical analysis of the slices using antibodies to Rip, GFAP and Iba-1 protein markers of oligodendrocyte, astroglia and microglia, respectively.Results. It was shown that the combined effect of OGD and lipopolysaccharide resulted in a significant release of the cytosolic enzyme LDH in culture medium, decrease of Rip-immunoreactivity and a pronounced reactive astro- and microgliosis in murine organotypic brain slice culture.Conclusions. Our model of PVL developed on cultured organotypic mice brain slices is novel and promising tool to study pathogenic mechanisms of cerebral white matter lesions and ways of neuroprotection in this pathology, including pharmacological agents and transplantation of stem cells.