bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiomyocyte-enriched USP20 ameliorates pathological cardiac hypertrophy by targeting STAT3-CARM1 axis
Authors: Yin Deling , Zhong Lingfeng , Dai Shanshan , Yu Fan , Shi Guo-Ping , Gong Qinyan , Zhang Yucong , Duan Jingsi , Lou Zhengyin , Tang Zhixuan , Gong Fuzhe , Chen Derong , Hou Liya , Chen Jinghai , Wang Jian-An 
Published: October 2024

Abstract

Pathological cardiac hypertrophy can lead to heart failure. The molecular mechanisms underlying pathological cardiac hypertrophy remain undefined. This study aimed to examine the role and molecular mechanisms of a deubiquitinating enzyme (DUB), ubiquitin-specific protease 20 (USP20) in cardiac hypertrophy. Hypertrophic hearts were conducted for RNA-sequencing to screen the expression profiles of DUBs. Cardiomyocyte-specific USP20 knockout and overexpression mouse models were generated to explore its function. Co-immunoprecipitation coupled with liquid chromatography-mass spectrometry/mass spectrometry were performed to screen potential USP20 substrates. Cleavage under targets and tagmentation assay with high-throughput sequencing was utilized to identify the potential downstream targets of STAT3. We identified cardiomyocyte-enriched USP20 is downregulated in cardiac hypertrophy. Cardiomyocyte-specific USP20 deficiency exacerbated cardiac hypertrophy induced by Angiotensin II and transverse aortic constriction, whereas cardiomyocyte-specific USP20 overexpression ameliorated the phenotype. We further identified STAT3 is a substrate of USP20 during cardiac hypertrophy through direct binding with DUSP2 domain. Mechanistically, USP20 removes K63 ubiquitin chains from STAT3 at the K177 site via its H645 active site, reducing STAT3 phosphorylation and nuclear translocation. This prevents STAT3 from binding to the coactivator-associated arginine methyltransferase 1 (CARM1) promoter, thereby promoting CARM1 transcription and improving cardiac hypertrophy. Importantly, we discover with STAT3 inhibitor stattic that STAT3 is a key substrate through which USP20 exerts its therapeutic effect on cardiac hypertrophy. These results elucidate a critical role for a novel USP20/STAT3/CARM1 axis in cardiomyocytes and an exciting new avenue study for therapies to treat cardiac hypertrophy.
Hemoptysis in a Complex Case: Unraveling Underlying Factors
Authors: Kang Jianxiong , Zhang Weihao , Wang Mu , Hua Peiyan , Wang Bin 
Published: October 2024

Abstract

Case presentation: A middle-aged female patient underwent right upper and middle lobe lung resection surgery seven years ago and has since experienced intermittent hemoptysis symptoms. Following chest examinations at our hospital, the patient was diagnosed with surgical ligation of the pulmonary artery. Concurrently, the patient has pulmonary aspergillosis, pulmonary tuberculosis, and bronchiectasis. Sequentially, the patient underwent bronchoscopy and percutaneous arterial embolization treatments at our hospital, but there was no improvement. Ultimately, surgical intervention was chosen. The mechanisms by which bronchial arteries, non-bronchial systemic arteries (NBSA), pulmonary aspergillosis, and pulmonary tuberculosis induce hemoptysis, along with strategies to address blood supply to the remaining lung post-pulmonary artery ligation, merit in-depth investigation. Conclusions: We deem this case exceptionally rare and of considerable research value; therefore, we conducted a thorough analysis and report.
Comparative Effectiveness of Bubble versus Ventilator-Derived Nasal Continuous Positive Airway Pressure on the Management of Transient Tachypnoea of the Newborn: A Retrospective Cohort Study in a Tertiary Care Hospital
Authors: TUTAK Ercan , ÇELİK Zeynep TUTAR 
Published: October 2024

Abstract

Background
Transient Tachypnoea of the Newborn (TTN) is a common cause of neonatal respiratory distress, often requiring respiratory support with Continuous Positive Airway Pressure (CPAP). This study compares the effectiveness of Bubble CPAP (BCPAP) and Ventilator-derived CPAP (VCPAP) in reducing NICU admissions in late preterm and term neonates with TTN.
Materials and Methods
This retrospective cohort study analysed 144 neonates born at or beyond 34 weeks' gestation with TTN who received either BCPAP (n = 70) or VCPAP (n = 74) in the delivery room. Outcomes included NICU admissions, length of hospital stay, intubation rates, surfactant use, and complications.
Results
There were no significant differences between the groups in NICU admissions (p > 0.05), hospital stay, intubation rates, or surfactant use. No cases of sepsis, necrotizing enterocolitis, or pneumothorax were observed.
Conclusion
Both BCPAP and VCPAP were equally effective in managing TTN, with no notable difference in clinical outcomes or safety. Further prospective studies are required to validate these findings.
Digital Economy, Logistics Development and the Increase of Farmers’ Income: An Empirical Analysis Based on the Eastern Economic Region of China
Authors: Miao Xiaohong , Li Zhongbin , Ning Manxiu , Xu Anxin , Wu Zhilong 
Published: October 2024

Abstract

In the context of China’s “Rural revitalization via digital commerce”, the study explored the impact of the digital economy on farmers’ income and the path mechanism of logistics development in it. Based on the panel data of 71 cities in the Eastern Economic Region of China from 2013 to 2021, this paper calculated the level of the digital economy and used the level of logistics development as a moderating variable. Then using the basic transmission mechanism and the heterogeneous transmission mechanism to empirically analyse the impact of the digital economy on the income of farmers, exploring the non-linear link between the digital economy and farmers’ income. The analysis showed that: (1) the development of digital economy has a significant positive impact on farmers’ income. (2) the impact of the digital economy on the advancement of logistics development, showed a delayed phenomenon. (3) the digital economy’s indirect effect on farmers’ income growth also showed a lag. In this way, this study verified the practical significance of “Rural revitalization via digital commerce”, and put forward the corresponding recommendations for promoting the development of the digital economy and rural logistics.
Fasting and Diabetes: An Interventional Approach to Enhance Knowledge and Self-Care during Ramadan
Authors: Firdausa Sarah , Syahrizal Dedy , Rachmah Rachmah , Suhanda Rachmad , Zulkarnain Zulkarnain , Adewiah Siti , Munirwan Haris , Meutia Firdalena , Vonna Azizah , Suryawati Suryawati , Zufry Hendra , Ekadamayanti Agustia Sukri , Sucipto Krishna W , Ardiani Deasy , Suryantoro Satriyo Dwi , Maulana Hanapi , Ghufran Ghufran 
Published: October 2024

Abstract

Background. The aim of this study was to assess the impact of educational interventions on the knowledge and self-care practices of diabetes patients who fast during Ramadan. People diagnosed with type 2 diabetes were selected from outpatient clinic at Dr. Zainoel Abidin Hospital. Educational intervention was delivered before and during Ramadan. Disease knowledge and self-care practices were assessed by using Diabetes Knowledge Questionnaire and Diabetes Self-Management Questionnaire. Chi-square test was used to analyse the changes before and after educational intervention.
Results. There were 186 diabetes patients involved in this study with average age of 59.77 ± 9.64 years, body mass index was overweight at 24.78 ± 3.62 kg/m2 and poor HbA1c at 9.21 ± 2.62 %. The DKQ scores spotted a substantial improvement, with a vast majority (92.47%) achieving the good knowledge category compared to the previous percentage of 30.65%. There was also a significant change in DMSQ scores before and after the educational intervention. Pre-intervention, 83.87% of DMSQ scores were optimal self-care practice, while 16.13% were suboptimal. After intervention, it changed significantly, with 94.08% of those characterized as having optimal self-care habits. Three items of DMSQ subscales showed significant changes which were eating behavior (p 0.046), medication taking (p 0.001), and glucose monitoring (p 0.001).
Conclusion. The study suggests that the educational intervention effectively improved both the knowledge and self-care practices of diabetes patients, as evidenced by the significant positive changes in knowledge and self-practice scores. This implies the potential for such interventions to contribute positively to the management and overall well-being of individuals with diabetes.
Evaluating treatment outcome of Glioblastoma with Stupp’s regimen: an experienced in single Institute
Authors: Van Dang Nguyen , Thu Nhung Nguyen Thi , Huy Phan Nguyen , Thi Hang Nguyen , Cam Thanh Do 
Published: October 2024

Abstract

Background
The current conventional treatment approach for newly diagnosed GBM entails the complete removal of the tumor, followed by the implementation of Stupp's procedure. We assessed the results of Stupp's treatment protocol and examined certain prognostic markers associated with survival, which could offer empirical evidence in the treatment of GBM.
Methods
A total of 64 patients diagnosed with newly developed glioblastoma underwent treatment with irradiation and TMZ at VNCH from January 2020 to September 2022. The study provided information on the demographic and clinical features of the patients, as well as their overall survival (OS) and progression-free survival (PFS) outcomes. The analysis of survival and related variables involved the utilization of Kaplan-Meier curves, Cox regression, and the log-rank test.
Results
The retrospective analysis comprised 64 participants. The vast majority patients were in favorable performance status. The median OS and PFS were 24.33 and 9.39 months, respectively. Factors as female patients, GTR/STR, time to start RT within 8 weeks postoperative, no progressive disease after CCRT, no dexamethasone required and Ki67 level below 15% experienced increased OS. RegardingPFS, characteristics such as age < 40 years old, GTR/STR and no disease progression following CCRT were substantially related with improved survival. Nearly half of patients received TMZ 50mg/m2 in combination with Bevacizumab following the initial progressive illness.
Conclusion
Multidisciplinary collaboration, as well as advancements in diagnostic and customized treatment strategies, are critical in the treatment of GBM patients. In actual life, completing the entire Stupp's protocolsignificantly improves GBM survival.
Nutritional composition, mineral profile and phytochemical content of new and established cultivars of chickpea as effected by cropping year
Authors: . Prashansa , Kumar Rajendra , Yadav Neelam 
Published: October 2024

Abstract

The study investigates the proximate composition, non-nutrient phenols, as well as mineral profile of various chickpea cultivars. Eight cultivars, comprising four desi (BG-3062, BG-20211, BG-1053, and K-850) and four kabuli (BG-3022, BG-2024, BG-1103, BG-1108) varieties, were analyzed. The proximate composition of chickpea was assessed by AOAC method and values depicted that all cultivars had appreciable amount of protein. However, there was significant difference in protein (19.13% - 25.36%) between cultivars. The non-nutrient analysis showed total phenolic content (TPC)ranged from 101- 276 mg GAE/100g and total flavonoid content (TFC) from 0.100-0.173 mg/g. Phytate content varied between 579-891.6 mg/100g. Phenol and phytate content were higher in desi cultivars than kabuli. Mineral analysis of chickpea was done by ICP-OES method and result highlighted significant differences in calcium, chromium, and other essential minerals. Newer cultivars BG-20211 had highest iron content as well as good amount of zinc hence can be screened out for this quality. The study also compared nutritional profile of four established cultivars of chickpea over a 10 years (2009 and 2019) cropping interval. There were significant changes in protein and mineral content in established chickpea cultivars in both cropping years; whereas TPC content was in the same order of magnitude. A significant increase in phytate content was reported in the year 2019 in three out of four established cultivars. The findings suggest that these chickpea cultivars possess diverse nutritional properties and has significant impact of climate change. This emphasizes the need of targeted breeding and agricultural practices to enhance chickpea quality.
Diagnostic Accuracy of Combined T2-weighted MR Imaging and H1MR Spectroscopy for Prostate Cancer Detection
Authors: Idrees Ibrahim Nasir 
Published: October 2024

Abstract

Background
The primary objective was to evaluate the diagnostic accuracy of combining T2-weighted MR imaging and H1MR Spectroscopy in detecting prostate cancer, with histopathology serving as the reference standard.
Methods
The study included 125 male patients aged 44–78, who presented with clinical suspicion of prostate cancer (e.g., gland asymmetry, nodule, induration, adhesion, or palpable seminal vesicles). Patients with previous surgeries, hormonal or radiation therapy, or a biopsy within the last 12 weeks were excluded. MR imaging and H1MR Spectroscopy were conducted using a 1.5 Tesla scanner, and histopathology was used as the gold standard.
Results
The mean patient age was 63.17 ± 8.47 years. Of the 125 patients, 54 were true positives, 7 were false positives, 49 were true negatives, and 15 were false negatives. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were 78.26%, 87.5%, 88.52%, 76.56%, and 82.4%, respectively.
Conclusion
The combination of T2-weighted MR imaging and H1MR Spectroscopy demonstrates high diagnostic accuracy in detecting prostate cancer. This technique, pending further validation, may serve as a reliable non-invasive diagnostic method in clinical practice.
Establishment of gender related 99th percentile values for cardiac troponin-T among young and middle-aged adult Egyptians
Authors: Tork Ahmed , Labib Mohamed , Rabea Abeer , Ali Abeer 
Published: October 2024

Abstract

Background
Cardiac troponins are the preferred biomarkers for the diagnosis of acute myocardial infarction (AMI). The stable nature of cardiac troponin T (cTnT) in the circulation and the worldwide available single standardized assay make it a more precise biomarker of AMI. The manufacturer’s proposed upper reference limit (URL) for the high sensitive (hs)-cTnT assay does not take into consideration patients’ gender, age or race. The study aimed at establishing and comparing gender-specific upper reference limit for cardiac troponin-T among apparently healthy young adult Egyptians.
Subjects and methods
240 adult Egyptians (120 males and 120 females) participated in this study. Serum cTnT was determined using a commercially available hs-cTnT electrochemiluminescent immunoassay. The gender related 99th percentile values were then calculated to represent the URLs for cTnT according to the CLSI and IFCC approved guidelines.
Results
Serum cTnT was significantly higher in males compared to females (p < 0.001). The 99th percentile URL for cTnT in females (12.58 ng/L) was lower than that of males (19.55 ng/L). Upon dividing them according to an age cutoff value of 36 years, serum cTnT level was significantly lower among those aged < 36 years old (n = 119) compared to those aged 36 years old or more (n = 121) (p < 0.001), with a proposed 99th percentile URL after including outliers of 18.1 ng/L for the former and 16.42 ng/L for the latter, while after excluding outliers the URL was 10.0 ng/L for the former and 12.6 ng/L for the latter. Moreover, a significantly higher serum cTnT value among males compared to females was only noticed in those aged less than 36 years old (p = 0.001).
Conclusion:
An adult patient in early diagnosis of AMI by increasing both assay’s specificity among adult The use of age and gender specific 99th percentile value as an URL of hs-cTnT assay would benefit males (decreasing over-diagnosis) and assay’s sensitivity among adult females (decreasing under-diagnosis).
Incorporation of different particle-size peanut shell powder in wheat flour: effect on the dough and cookie characteristics
Authors: ALKIŞ Ülgen İlknur KONAK , EREM Fundagül 
Published: October 2024

Abstract

Peanuts are the most produced and processed industrial crops worldwide. Therefore, a considerable amount of waste is generated during its processing. In this study, the effect of peanut shells ground to different sizes (212, 500, and 800 µm) on the physical, chemical, techno-functional, and textural properties of cookies was investigated. The hardness of the dough increased and the stickiness and the dough strength decreased as the particle size decreased; however, no significant differences were observed in the textural parameters between the doughs obtained with 500 µm and 800 µm aperture-sized peanut shell powder. The addition of peanut shells to cookies was found to increase the crude fiber content by 85-100-fold, the total phenolic content by 1.5-1.8-fold, and the antioxidant activity by 2-2.4-fold. The incorporation of peanut shell powder in cookies decreased the thickness by 8–15% but increased the spread ratio by 18–33% compared to the control cookies. The hardness and the fracturability of cookies decreased by about 27–47% and 1–5%, respectively, as the particle size of peanut shell powder increased. The results suggest that peanut shells could be a noteworthy source of fiber and phenolic compounds in functional cookie production.
Impact of Lower Limb Muscle Strength and Power Asymmetry on Multidirectional Speed in Female Soccer Players
Authors: Skalski Dariusz , Prończuk Magdalena , Łośinska Kinga , Spieszny Michał , Kostrzewa Maciej , Aschenbrenner Piotr , Maszczyk Adam 
Published: October 2024

Abstract

The aim of the present study was to examine and determine the impact of asymmetry of muscle strength and power between the right and left lower limbs on running speed with changes of direction (multidirectional speed) in female football players. 20 right-footed elite female soccer players from the Ekstraliga participated in the study. Statistical analysis indicates that in running speed with change of direction in the 505 Right and 505 Left tests (group criterion: MVSLJ), players in the group with higher asymmetry (G2) achieved higher change-of-direction running speeds than those in the group with lower asymmetry (G1). A one-way ANOVA of running speeds between groups G1 and G2 (group criterion: PPLP) indicate statistically significant differences in running speed between groups in the Zigzag and 505 Right tests. Players in the group with higher asymmetry (G2) ran faster in the change-of-direction sections of the 505 Right. Based on the results collected during the study on the impact of differences in strength and power between the lower limbs on change-of-direction sprint speed in professional female soccer players, it was observed that players with smaller asymmetry achieved lower change-of-direction running speeds than those with greater asymmetry. Moreover, all observed significant differences in running speed tests between the analyzed groups were significantly correlated with the percentage differences in lower limb power within these groups.
Primary Klebsiella pneumoniae in Lumbar Spine: Case Report and Literature Review
Authors: Liu Wenying , Wang Yafang , Shi Juanhong , Dong Guixia 
Published: October 2024

Abstract

Background:
Klebsiella pneumoniae infections are predominantly observed in respiratory medicine, but their manifestation in the spine is exceedingly rare. Such infections can be easily manifestation as spinal tuberculosis or other similar infections, resulting in non-standardized treatments and an increased risk of adverse drug reactions. This study aims to explore the standardization and safety of early diagnosis and treatment of Klebsiella pneumoniae infections in the spine.
Case presentation:
We report the case of a 38-year-old female who presented with an acute onset of lower back pain. Initially diagnosed with spinal tuberculosis at a local community hospital, she was treated with anti-tuberculosis medications, which failed to alleviate her symptoms. Upon re-evaluation with enhanced MRI and additional laboratory tests, a core needle biopsy (CNB) was performed under local anesthesia, confirming Klebsiella pneumoniae as the causative pathogen. Following appropriate antibiotic treatment, the patient's pain symptoms significantly improved. Follow-up lumbar MRI indicated the complete resolution of vertebral and paravertebral abscesses. During a one-year follow-up period, the patient experienced no further complications and successfully conceived, delivering a healthy male infant.
Conclusions:
This case report highlights the rarity and significance of primary Klebsiella pneumoniae infections in the lumbar spine, emphasizing the need for heightened awareness among clinicians. Despite its infrequency, K. pneumoniae should be considered in differential diagnoses for spinal infections, particularly when tuberculosis is suspected. Early and accurate diagnosis through techniques such as core needle biopsy is crucial for effective management. Our findings suggest that tailored antibiotic therapy, combined with diligent long-term follow-up using MRI, can lead to successful outcomes and minimize the risk of recurrence. Given the challenges posed by potential drug resistance and the complexity of spinal infections, further research is essential to establish standardized treatment protocols and improve patient care in this domain.
A phase 2 randomized, placebo-controlled trial of inulin for the prevention of gut pathogen colonization and infection in the intensive care unit
Authors: Park Heekuk , Lynch Elissa , Tillman Alice , Lewis Kristen , Jin Zhezhen , Uhlemann Anne-Catrin , Abrams Julian A. , Freedberg Daniel E. 
Published: October 2024

Abstract

Objective
Patients admitted to the intensive care unit (ICU) often have gut colonization with pathogenic bacteria and such colonization is associated with increased risk for death and infection. We conducted a trial to determine whether a prebiotic would improve the gut microbiome to decrease gut pathogen colonization and decrease downstream risk for infection among newly admitted medical ICU patients.
Design
Randomized, double-blind, placebo-controlled trial with two prebiotic dose arms.
Setting
Two medical ICUs at a single center.
Patients
Adults who were admitted to the medical ICU for sepsis and were receiving broad-spectrum antibiotics.
Intervention
Participants were randomized 1:1:1 to placebo, inulin 16 grams/day, or inulin 32 grams/day for seven days.
Measurements and main results
The trial primary outcome was a surrogate measure for gut colonization resistance, namely the within-individual change from ICU admission to Day 3 in the relative abundance of short chain fatty acid (SCFA)-producing bacteria based on rectal swabs. Ninety participants were analyzed, including 30 in each study group. There was no difference between study groups in the relative abundance of SCFA-producing bacteria from ICU admission to ICU Day 3 (placebo: 0.0% change, IQR -8·0% to +7·4% vs. combined inulin: 0·0% change, IQR -10·1% to +4·8%; p=0·91). At end-of-treatment on ICU Day 7, inulin did not affect SCFA-producer levels, microbiome diversity, or rates of gut colonization with pathogenic bacteria. After 30 days of clinical follow-up, inulin did not affect rates of death or clinical infection. Patients who died or developed culture-proven infections had lower relative abundance of SCFA-producing bacteria at ICU admission compared to those who did not (p=0.03).
Conclusions
Prebiotic fiber had minimal impact on the gut microbiome in the ICU and did not improve clinical outcomes. Microbiome restitution therapies may face significant challenges in the ICU.
Trial registration: Clinicaltrials.gov: NCT03865706. Registered 10/14/19