bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex hormone‐binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer
Authors: Cheng Chao‐Wen , Chang Che‐Chang , Patria Yudha Nur , Chang Ruei‐Ting , Liu Yun‐Ru , Li Fu‐An , Shih Hsiu‐Ming , Lin Ching‐Yu 
Published: November 2017

AbstractThe use of blood plasma biomarkers in gastric cancer (GC) management is limited due to a lack of reliable biomarkers. An LC‐MS/MS assay and a bioinformatic analysis were performed to identify blood plasma biomarkers in a GC discovery cohort. The data obtained were verified and validated by western blotting and an ELISA in an independent study cohort. A label‐free quantification analysis of the MS data using PEAKS7 software found that four plasma proteins of apolipoprotein C‐1, gelsolin, sex hormone‐binding globulin (SHBG), and complement component C4‐A were significantly overexpressed in GC patients. A western blot assay of these plasma proteins showed that only SHBG was consistently overexpressed in the patient group. ELISA measurement of SHBG blood plasma levels confirmed that the patient group had significantly higher SHBG levels than the control group. SHBG levels in the patient group remained significantly higher after being stratified by gender, age, and disease stage. These findings show that LC‐MS/MS is powerful and highly sensitive for plasma biomarker discovery, and SHBG could be a potential plasma biomarker for GC management.
New WS9326A Derivatives and One New Annimycin Derivative with Antimalarial Activity are Produced by Streptomyces asterosporus DSM 41452 and Its Mutant
Authors: Zhang Songya , Zhu Jing , Zechel David L. , Jessen‐Trefzer Claudia , Eastman Richard T. , Paululat Thomas , Bechthold Andreas 
Published: November 2017

AbstractIn this study, we report that Streptomyces asterosporus DSM 41452 is a producer of new molecules related to the nonribosomal cyclodepsipeptide WS9326A and the polyketide annimycin. S. asterosporus DSM 41452 is shown to produce six cyclodepsipeptides and peptides, WS9326A to G. Notably, the compounds WS9326F and WS9326G have not been described before. The genome of S. asterosporus DSM 41452 was sequenced, and a putative WS9326A gene cluster was identified. Gene‐deletion experiments confirmed that this cluster was responsible for the biosynthesis of WS9326A to G. Additionally, a gene‐deletion experiment demonstrated that sas16 encoding a cytochrome P450 monooxygenase was involved in the synthesis of the novel (E)‐2,3‐dehydrotyrosine residue found in WS9326A and its derivatives. An insertion mutation within the putative annimycin gene cluster led to the production of a new annimycin derivative, annimycin B, which exhibited modest inhibitory activity against Plasmodium falciparum.
NAD+‐Dependent Dehydrogenase PctP and Pyridoxal 5′‐Phosphate Dependent Aminotransferase PctC Catalyze the First Postglycosylation Modification of the Sugar Intermediate in Pactamycin Biosynthesis
Authors: Hirayama Akane , Chu Jinmiao , Goto Ena , Kudo Fumitaka , Eguchi Tadashi 
Published: November 2017

AbstractThe unique five‐membered aminocyclitol core of the antitumor antibiotic pactamycin originates from d‐glucose, so unprecedented enzymatic modifications of the sugar intermediate are involved in the biosynthesis. However, the order of the modification reactions remains elusive. Herein, we examined the timing of introduction of an amino group into certain sugar‐derived intermediates by using recombinant enzymes that were encoded in the pactamycin biosynthesis gene cluster. We found that the NAD+‐dependent alcohol dehydrogenase PctP and pyridoxal 5′‐phosphate dependent aminotransferase PctC converted N‐acetyl‐d‐glucosaminyl‐3‐aminoacetophonone into 3′‐amino‐3′‐deoxy‐N‐acetyl‐d‐glucosaminyl‐3‐aminoacetophenone. Further, N‐acetyl‐d‐glucosaminyl‐3‐aminophenyl‐β‐oxopropanoic acid ethyl ester was converted into the corresponding 3′‐amino derivative. However, PctP did not oxidize most of the tested d‐glucose derivatives, including UDP‐GlcNAc. Thus, modification of the GlcNAc moiety in pactamycin biosynthesis appears to occur after the glycosylation of aniline derivatives.
MCP2 activates NF‐κB signaling pathway promoting the migration and invasion of ESCC cells
Authors: Zhou Jian , Zheng Shutao , Liu Tao , Liu Qing , Chen Yumei , Tan Doudou , Ma Rong , Lu Xiaomei 
Published: November 2017

AbstractMCP2, aliased CCL8, has been suggested to be implicated in the metastasis of cancer cells; however, no direct evidence has been established in esophageal squamous cell carcinoma (ESCC). In our present study, to investigate the role MCP2 played in the metastasis of ESCC cells; in vitro cell co‐culture system was established. Wound‐healing and Transwell assays were used to evaluate the migratory and invasive variation of ESCC cells before and after treatment with recombinant human MCP2. It was shown that MCP2 was able to activate the NF‐κB signaling pathway inducing the epithelial‐mesenchymal transition (EMT) and promoting the migration and invasion of ESCC cells in vitro. Our study provides an alternative working mechanism for M2 macrophage mediated the metastasis in tumor microenvironment in ESCC.
Peri‐procedural myocardial infarction: Plaques and patients “at‐risk”
Authors: Goldstein James A. 
Published: November 2017

Key Points

Patients with acute coronary syndrome are at risk for peri‐procedural myocardial infarction (PMI)
Lipid‐laden plaques with thinned disrupted fibrous caps are most prone to PMI
PMI is associated with worse outcome over time, though whether such damaging events are causal or instead reflect patients prone to future instability from non‐culprit vulnerable plaques requires further delineation
What can intracoronary pressure measurements tell us about CFR? Now comes pb‐CFR
Authors: Nakamura Kenta , Dean Larry S. 
Published: November 2017

Key Points

Pressure‐bound (pb) coronary flow reserve (CFR) is a novel estimation of CFR, calculated from easily obtained baseline and hyperemic pressure assessment
Validation of pb‐CFR in previously reported lesions showed accuracy of 84% to CFR with sensitivity of 96% and specificity of 60%
FFR >0.75 and low pb‐CFR in DEFER was associated with increased angina and complications compared to high pb‐CFR and this risk was not modified by elective revascularization.
Recurrent coarctation of aorta after Norwood operation in single ventricle patients; the case of the missing ideal stent
Authors: Parekh Dhaval R. , Krajcer Zvonimir 
Published: November 2017

Key Points

Recurrent coarctation postoperative Norwood is poorly tolerated in single ventricle patients.
Although transcatheter balloon angioplasty is considered first line, it often leads to inadequate or non‐durable relief of obstruction.
Transcatheter stent implantation provides excellent relief of obstruction but necessitates staged redilation to account for somatic growth in children.
Stent technology, delivery systems, and procedural techniques will need to evolve to obviate reported adverse events mainly due to patient size.
Morphology‐Controlled Synthesis of Molybdenum Disulfide Wrapped Single‐Walled Carbon Nanotubes for the Hydrogen Evolution Reaction
Authors: Chen Lin , Zeng Min , Zhang Wenqing , He Lin , He Wenya , Yang Fengchun , Zhang Xin 
Published: November 2017

AbstractThe synthesis of new nanohybrid catalysts with a tunable structure and the exploration of their synergistic behaviors have captured substantial attention. In this work, amorphous molybdenum disulfide/single‐walled carbon nanotubes (MoS2/SWNTs) composites are synthesized by a facile hydrothermal process with the assistance of l‐cysteine. We varied the mass ratio of sodium molybdate (the in situ Mo source) to SWNTs, this method provides a well‐defined pathway to enable the morphological control over the evolution of MoS2 from nanospheres to nanofilaments, nanorods, and nanosheets. Among the as‐obtained samples, the MoS2/SWNTs (1:1) hybrids, which displayed a 3 D architecture that consisted of nanorods, exhibited the highest activity in the hydrogen evolution reaction (HER). Compared with pure MoS2, the MoS2 nanosheets that decorate the SWNTs have more highly exposed active edges, and the SWNTs in the center of the MoS2/SWNTs nanorods can enhance the conductivity. We regulated the ratio between MoS2 and SWNTs to obtain a balance between the number of active sites and the conductivity of MoS2/SWNTs. Here, MoS2/SWNTs nanorods exhibited a low overpotential of 195 mV at 20 mA cm−2 and a low Tafel slope of 41 mV dec−1 for MoS2‐based HER catalysts.
In Situ Confined Synthesis of Ti4O7 Supported Platinum Electrocatalysts with Enhanced Activity and Stability for the Oxygen Reduction Reaction
Authors: Duma Alemayehu Dubale , Wu Yi‐Chen , Su Wei‐Nien , Pan Chun‐Jern , Tsai Meng‐Che , Chen Hung‐Ming , Lee Jyh‐Fu , Sheu Hwo‐Shuenn , Ho Van Thi Thanh , Hwang Bing‐Joe 
Published: November 2017

AbstractHerein, an in situ confined synthesis of highly conductive titanium oxide supported Pt catalysts is successfully employed. The method involves crystallization and formation of TiO2 particles inside the inert silica shell introduced by using hexamethyldisilazane (HMDS). The conductivity of titania is found to be greatly enhanced by increased annealing temperature, whereas the inevitable decrease in the specific surface area was effectively inhibited by the confining silica shell derived from HMDS. The optimal catalyst (20Pt 850 FHST), exhibited better oxygen reduction reaction (ORR) activity with a maximum current density of 0.049 mA cm−2 at 0.9 V (vs. normal hydrogen electrode (NHE)) and an onset potential of 0.86 V (vs. NHE). The prolonged cycling test demonstrated superior stability compared with Pt/C (JM 20). Moreover, X‐ray absorption spectroscopy asserts that the increased electron population in the Pt 5d orbital, emanating from the strong metal–support interaction, improves the electrochemical performance in the ORR. The in situ HMDS‐assisted synthesis can also be extended to other oxide‐supported catalytic systems.
Pd‐Catalyzed β‐C(sp3)−H Arylation of Propionic Acid and Related Aliphatic Acids
Authors: Ghosh Kiron K. , van Gemmeren Manuel 
Published: November 2017

AbstractA generally applicable Pd‐catalyzed protocol for the β‐C(sp3)−H arylation of propionic acid and related α‐branched aliphatic acids is reported. Enabled by the use of N‐acetyl‐β‐alanine as ligand our protocol delivers a broad range of arylation products. Notably, the highly challenging substrate, propionic acid, which lacks any acceleration through the Thorpe–Ingold effect, can be employed as substrate with synthetically useful yields. Furthermore, the scalability and synthetic applicability of the protocol are demonstrated.
Business Forecasting of Double‐trend Time Series: An Improved PLS‐based Time‐varying Weight Combination Approach
Authors: Luo Biao , Wan Liang , Li Tieshan , Liang Liang 
Published: November 2017

AbstractBusiness forecasting with double‐trend time series (long‐term trends and seasonal volatility) has been challenging due to its complexity. Neither a single time series model nor a fixed‐weight combination approach can fully capture the comprehensive information. We address this issue by proposing an improved partial least squares (PLS) based time‐varying weight combination approach. The proposed method can handle the relations both between the single models involved and between single models and time ordering with time‐varying weights. The test on 20 simulated datasets demonstrates the better and more robust performance of the method. We also apply it to three real datasets. The results show that our approach represents a significant improvement over the existing methods in terms of data fitness and prediction accuracy. Copyright © 2017 ASAC. Published by John Wiley & Sons, Ltd.
Effects of starch nanoparticles on phase inversion of Pickering emulsions
Authors: Ogunlaja Sileola B. , Pal Rajinder , Sarikhani Kaveh 
Published: November 2017

AbstractUnderstanding the effects of interfacial additives such as surface‐active nanoparticles on phase inversion of Pickering emulsions is important from a practical point of view. In this work, we studied the effects of surface‐active starch nanoparticles on catastrophic phase inversion of Pickering emulsions by continuous addition of a dispersed phase. Two types of experimental‐grade starch nanoparticles were used: hydrophilic starch nanoparticles (HSNP) and hydrophobic starch nanoparticles (HOSNP). The dynamic oil‐water interfacial tension was measured using the pendant drop method at varying starch concentrations in the aqueous phase while the contact angles were measured using the sessile drop method of the Axisymmetric Drop Shape Analysis‐Profile (ADSA‐P). Both types of starch nanoparticles (HSNP and HOSNP) were effective in delaying the phase inversion of emulsions from water‐in‐oil (W/O) type to oil‐in‐water (O/W) type. This delay in phase inversion was directly correlated with the concentration of starch nanoparticles. The interfacial tension decreased as the drop aged at a given starch nanoparticle concentration. The contact angles for both types of starch nanoparticles were within the intermediate wettability range that confirmed the irreversible adsorption of starch nanoparticles at the oil/water interface leading to an increased stability of emulsions.
Testing the heteroscedastic error structure in quantile varying coefficient models
Authors: Gijbels Irène , Ibrahim Mohammed A. , Verhasselt Anneleen 
Published: November 2017

AbstractIn mean regression the characteristic of interest is the conditional mean of the response given the covariates. In quantile regression the aim is to estimate any quantile of the conditional distribution function. For given covariates, the conditional quantile function fully characterizes the entire conditional distribution function, in contrast to the mean which is just one of its characteristic quantities. Regression quantiles substantially out‐perform the least‐squares estimator for a wide class of non‐Gaussian error distributions. In this article we consider quantile varying coefficient models (VCMs) that are an extension of classical quantile linear regression models, in which one allows the coefficients to depend on other variables. We consider VCMs with various structures for the variance of the errors (the variability function) in order to allow for heteroscedasticity. For longitudinal data, the time (T) dependent coefficient functions in the signal and the variability functions are estimated with P‐splines (Penalized B‐splines). Consistency of the proposed estimators is proved. Further, likelihood‐ratio‐type tests are considered for comparing the variability functions. The performance of the testing procedure is illustrated on simulated and real data.The Canadian Journal of Statistics46: 246–264; 2018 © 2017 Statistical Society of Canada
Probing nitrite ion dynamics in NaNO2 crystals by solid‐state 17O NMR
Authors: Dai Yizhe , Hung Ivan , Gan Zhehong , Wu Gang 
Published: November 2017

AbstractWe report a solid‐state 17O (I = 5/2) NMR study of the nitrite ion dynamics in crystalline NaNO2. Variable temperature (VT) 17O NMR spectra were recorded at 3 magnetic fields, 11.7, 14.1, and 21.1 T. The VT 17O NMR data suggest that the ion in the ferroelectric phase of NaNO2 undergoes 2‐fold flip motion about the crystallographic b axis and the corresponding rotational barrier is 68 ± 5 kJ mol−1. We also obtained a 2D 17O EXSY spectrum for a stationary sample of NaNO2 at 250 K, which, in combination with 1D 17O NMR spectral analyses, allowed precise determination of the relative orientation between the 17O quadrupolar coupling and chemical shift tensors in the molecular frame of reference. The experimentally determined 17O NMR tensors for NaNO2 were in agreement with quantum chemical calculations produced by a periodic DFT code BAND.
Sexual functioning among young adult cancer patients: A 2‐year longitudinal study
Authors: Acquati Chiara , Zebrack Brad J. , Faul Anna C. , Embry Leanne , Aguilar Christine , Block Rebecca , Hayes‐Lattin Brandon , Freyer David R. , Cole Steve 
Published: November 2017

BACKGROUNDCancer‐related sexual dysfunction has been reported among adolescents and young adults (AYAs); however, its prevalence over time has not been examined. This longitudinal study investigated sexual dysfunction in AYAs over the course of 2 years after the initial diagnosis.METHODSYoung adult patients (18‐39 years old) completed the Medical Outcomes Study Sexual Functioning Scale within the first 4 months of their diagnosis (n = 123) and again 6 (n = 107) and 24 months later (n = 95). An ordered multinomial response model analyzed changes in the probability of reporting sexual dysfunction over time and the independent effects of demographic, clinical, and psychosocial variables.RESULTSMore than half of the participants reported sexual functioning to be problematic at each assessment. The probability of reporting sexual dysfunction increased over time (P < .01) and was greater for cancer patients who were female (P < .001), older (P < .01), married or in a committed relationship (P < .001), treated with chemotherapy (P < .05), and reporting comorbid psychological distress (P < .001) and lower social support (P < .05). For women, being in a relationship increased the likelihood of reporting sexual problems over time; for men, the likelihood of reporting sexual problems increased regardless of their relationship status.CONCLUSIONSA substantial proportion of young adults report ongoing problems with sexual functioning in the first 2 years after their cancer diagnosis. These findings justify the need to evaluate and monitor sexual functioning throughout a continuum of care. Cancer 2018;124:398‐405. © 2017 American Cancer Society.
Impact of chronic hepatitis C virus infection on the survival of patients with oropharyngeal cancer
Authors: Economides Minas P. , Amit Moran , Mahale Parag S. , Hosry Jeff J. , Jiang Ying , Bharadwaj Uddalak , Sturgis Erich M. , Torres Harrys A. 
Published: November 2017

BACKGROUNDAlthough an association between hepatitis C virus (HCV) infection and oropharyngeal cancers (OPCs) has been reported, to the authors' knowledge the clinical significance of this epidemiological finding remains unknown. Therefore, the authors analyzed the oncologic outcomes of HCV‐infected patients with OPCs.METHODSIn this retrospective cohort study, all patients with OPCs who were seen at The University of Texas MD Anderson Cancer Center between January 2004 and December 2015 were reviewed. HCV infection was defined as detectable HCV RNA in the serum. Five‐year overall survival and progression‐free survival rates were compared between patients infected with HCV and those not infected.RESULTSA total of 161 patients were examined. The majority of the patients were white (141 patients; 88%) and male (132 patients; 82%) and had TNM stage III or IV disease (147 patients; 91%). The OPC involved the tonsils (83 patients; 52%), base of the tongue (67patients; 42%), or the soft palate (11 patients; 7%). The median follow‐up after an OPC diagnosis was 3 years (range, 1‐13 years). HCV‐infected patients (25 patients) and HCV‐uninfected patients (136 patients) were comparable with regard to smoking and alcohol status. In multivariate analysis, HCV was associated with increased cancer‐specific mortality (hazard ratio, 2.15; 95% confidence interval, 1.08‐6.85 [P = .02]) and risk of OPC progression (hazard ratio, 5.42; 95% confidence interval, 2.64‐11.14 [P = .0008]) independent of age and cirrhosis status. Antivirals were administered after the diagnosis of OPC in 8 of the 25 HCV‐infected patients (32%). HCV‐infected patients who received antivirals were found to have better 5‐year overall survival (70% vs 12%; P = .005) and progression‐free survival (72% vs 19%; P = .005) compared with patients who did not.CONCLUSIONSThe early detection of HCV is important in patients with OPC because this infection may affect their oncologic outcomes. Cancer 2018;124:960‐5. © 2017 American Cancer Society.
Microsecond Timescale Protein Dynamics: a Combined Solid‐State NMR Approach
Authors: Rovó Petra , Linser Rasmus 
Published: November 2017

AbstractConformational exchange in proteins is a major determinant in protein functionality. In particular, the μs–ms timescale is associated with enzymatic activity and interactions between biological molecules. We show here that a comprehensive data set of R1ρ relaxation dispersion profiles employing multiple effective fields and tilt angles can be easily obtained in perdeuterated, partly back‐exchanged proteins at fast magic‐angle spinning and further complemented with chemical‐exchange saturation transfer NMR experiments. The approach exploits complementary sources of information and enables the extraction of multiple exchange parameters for μs–ms timescale conformational exchange, most notably including the sign of the chemical shift differences between the ground and excited states.
Effects of the methacrylate/acrylate monomers HEMA, TEGDMA, DEGDA, and EMA on the immune system
Authors: Alizadehgharib Sara , Östberg Anna‐Karin , Dahlgren Ulf 
Published: November 2017

AbstractIncomplete curing of dental fillings may lead to leakage of methacrylate/acrylate monomers, which may come in contact with different cells of the immune system in oral tissues. Very little is known about the different immunologic effects caused by these methacrylates/acrylates. The objective of the present study was to study if and how the methacrylate/acrylate monomers ethyl methacrylate (EMA) and diethylene glycol diacrylate (DEGDA) affect the immune system in vivo and in vitro in comparison to 2‐hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA). Human peripheral blood mononuclear cells were exposed to the different monomers (500 and 1000 μM) for 24 hr in vitro. BioPlex Pro™ assays were used for cytokine analysis. In vivo, BALB/c mice were immunized subcutaneously at the base of the tail with HEMA, TEGDMA, EMA, or DEGDA in combination with ovalbumin (OVA) in order to study adjuvant properties of the 4 monomers. Peripheral blood mononuclear cells exposed to DEGDA had viability less than 50% of the cells. A pattern was observed where the levels of most cytokines were elevated after exposure to HEMA or TEGDMA. Since that, many cells died after DEGDA‐exposure, the only observed cytokine secretion was a significantly increased production of interleukin‐18. In the in vivo experiments, all mice immunized with DEGDA died after the booster injection. Mice receiving OVA in combination with HEMA, TEGDMA, or EMA developed a higher immunoglobulin G anti‐OVA antibody levels compared to the group immunized with OVA alone. We could not demonstrate any significant difference in antibody levels among the mice receiving the various methacrylate/acrylate monomers. The different monomers affected the production, increase and decrease, of different cytokines in vitro but resulted also in vivo in increased antibody production and T‐cell activity.