bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural, electrical, leakage current, and magnetic characteristics of double perovskite Nd2NiTiO6
Authors: Malik Sujan , Dutta Abhigyan 
Published: September 2024

AbstractThis study investigates the structural, electrical, leakage current, and magnetic characteristics of the double perovskite material Nd2NiTiO6 synthesized through the sol–gel citrate auto‐ignition method. The Rietveld refinement of the X‐ray diffraction patterns confirmed a monoclinic symmetry (space group P21/n) with an ordered arrangement of alternate NiO6 and TiO6 octahedra. Impedance spectroscopic analysis exhibited the material's non–Debye‐type relaxation mechanism and semi‐conductive nature. AC conduction analysis revealed that the conduction of charge carriers occurs via the nonoverlapping small polaron hopping mechanism, consistent with Mott's variable range hopping model. Temperature‐ and frequency‐dependent dielectric measurements were interpreted using the Maxwell–Wagner interfacial polarization model. The analysis of thermal‐ and field‐dependent leakage currents established that conduction is ohmic, which the Schottky barrier model explains. Studies on magnetization ruled out the presence of magnetic ordering connected to indirect interaction between Ni2+ ions via Ti4+ ions and the rare‐earth cation (Nd3+) moment.
Adapting Complicated Grief Therapy for Use With People With Intellectual Disabilities: An Action Research Study
Authors: O'Riordan Damien , Conway Emma , Dodd Philip , Guerin Suzanne 
Published: September 2024

ABSTRACTBackgroundThere is established evidence of complicated grief among people with an intellectual disability. This paper describes the process of adapting complicated grief therapy (CGT) for this population.MethodAction research documented the adaptation of CGT. Qualitative methods included analysing meeting notes, reflective interviews with two members of the team involved in adapting the materials, and interviews with six professionals working in disability settings who reviewed the adapted materials.ResultsKey processes included adapting the standardised tools that form part of CGT and developing adapted approaches to abstract concepts related to death, dying and bereavement. Key therapeutic components such as imaginal revisiting and the role of significant others required adaptation for implementation with people with intellectual disabilities.ConclusionThe importance of adapting evidence‐based therapies for people with intellectual disabilities is emphasised. This research provides an adapted form of an established therapy for piloting with this population.
A meta‐analysis of the distance between lead‐implanted site and tricuspid valve annulus with postoperative tricuspid regurgitation deterioration in patients with left bundle branch area pacing
Authors: Karwiky Giky , Kamarullah William , Pranata Raymond , Achmad Chaerul , Iqbal Mohammad 
Published: September 2024

AbstractTricuspid regurgitation (TR) is a known complication of cardiac implantable electrical devices (CIEDs), with prevalences ranging from 10% to as high as 30%. Despite left bundle branch area pacing (LBBAP) has emerged as an alternative to the limits of His‐bundle pacing (HBP), the long‐term safety of this procedure, notably the worsening of TR after implantation, has yet to be thoroughly investigated. This meta‐analysis sought to determine the frequency of post‐LBBAP TR deterioration and identify the predictors, particularly the distance between lead‐implanted site and the tricuspid valve annulus (lead‐TA‐distance). A systematic literature search was conducted using PubMed, Europe PMC, and ScienceDirect for studies that reported the incidence of deterioration and measurement of TR grade at baseline and follow‐up following LBBAP, in addition to the differences in exposure between short and long lead‐TA‐distances. A total of three studies involving 480 participants were included in this meta‐analysis. The incidence of TR deterioration was 22%. Patients with TR deterioration also demonstrated a significantly shorter lead‐TA‐distance in comparison to the opposing group (MD: −5.74 mm (−0.70, −10.78); p < .001; I2 = 92.6%). The pooled results of three comparative studies suggest that participants in the longer lead‐TA‐distance group had a significant decrement in the likelihood of TR worsening (adjusted OR = 0.59 (0.36–0.96); p = .034; I2 = 79%). Multivariate analysis conducted in each of the included investigations supported the independence of the connection between lead‐TA‐distance and TR deterioration. A shorter lead‐TA‐distance was an independent risk factor for TR deterioration in individuals with post‐LBBAP implantation.
Carvacrol inhibits the progression of oral submucous fibrosis via downregulation of PVT1/miR‐20a‐5p‐mediated pyroptosis
Authors: Yu Cheng‐Chia , Hsieh Pei‐Ling , Chao Shih‐Chi , Liao Yi‐Wen , Yu Chuan‐Hang , Chueh Pin Ju , Peng Chih‐Yu , Lee Shiuan‐Shinn 
Published: September 2024

AbstractOral submucous fibrosis (OSF) is a precancerous condition in the oral cavity, which is closely related to the myofibroblast conversion of buccal mucosal fibroblasts (BMFs) after chronic consumption of areca nut. Emerging evidence suggests pyroptosis, a form of programmed cell death that is mediated by inflammasome, is implicated in persistent myofibroblast activation and fibrosis. Besides, numerous studies have demonstrated the effects of non‐coding RNAs on pyroptosis and myofibroblast activities. Herein, we aimed to target key long non‐coding RNA PVT1 with natural compound, carvacrol, to alleviate pyroptosis and myofibroblast activation in OSF. We first identified PVT1 was downregulated in the carvacrol‐treated fBMFs and then demonstrated that myofibroblast features and expression of pyroptosis makers were all reduced in response to carvacrol treatment. Subsequently, we analysed the expression of PVT1 and found that PVT1 was aberrantly upregulated in OSF specimens and positively correlated with several fibrosis markers. After revealing the suppressive effects of carvacrol on myofibroblast characterisitcs and pyroptosis were mediated by repression of PVT1, we then explored the potential mechanisms. Our data showed that PVT1 may serve as a sponge of microRNA(miR)‐20a to mitigate the myofibroblast activation and pyroptosis. Altogether, these findings indicated that the anti‐fibrosis effects of carvacrol merit consideration and may be due to the attenuation of pyroptosis and myofibroblast activation by targeting the PVT1/miR‐20a axis.
Distribution characteristics of Purkinje fibres in the canine left ventricle
Authors: Li Yunhao , Zhang Daoyang , Meng Yunfan , Zhang Jie , Zhang Qi , Zhang Ping , Zhang Yujie , Sun Mingyu , Wang Zulu , Liang Ming 
Published: September 2024

AbstractPurkinje‐related ventricular arrhythmias have been increasingly reported, and with the development of catheter ablation techniques, intervention for Purkinje‐related arrhythmias has been shown to be effective. The characteristics of Purkinje fibres orientation in the 12 canine left ventricles were observed at a gross level by staining the endocardium with Lugol's solution. Purkinje fibres were observed microscopically by HE, Masson's, PAS glycogen, and Cx40 immunohistochemical staining. Staining was successful, and the transverse orientation characteristics of Purkinje fibres were observed by Lugol's staining, and the longitudinal distribution was observed microscopically. The distribution of Purkinje fibres in the canine left ventricle is ‘graded’, ‘layered’, and ‘networked’, which can guide catheter ablation of Purkinje‐related ventricular arrhythmia.
The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: Implications for deep space missions, female crews, and potential antioxidant countermeasures
Authors: Yun Sanghee , Kiffer Frederico C. , Bancroft Grace L. , Guzman Caterina S. , Soler Ivan , Haas Harley A. , Shi Raymon , Patel Riya , Lara‐Jiménez Jaysen , Kumar Priya L. , Tran Fionya H. , Ahn Kyung Jin , Rong Yuying , Luitel Krishna , Shay Jerry W. , Eisch Amelia J. 
Published: September 2024

AbstractGalactic cosmic radiation (GCR) is an unavoidable risk to astronauts that may affect mission success. Male rodents exposed to 33‐beam‐GCR (33‐GCR) show short‐term cognitive deficits but reports on female rodents and long‐term assessment are lacking. We asked: What are the longitudinal behavioral effects of 33‐GCR on female mice? Also, can an antioxidant/anti‐inflammatory compound (CDDO‐EA) mitigate the impact of 33‐GCR? Mature (6‐month‐old) C57BL/6J female mice received CDDO‐EA (400 μg/g of food) or a control diet (vehicle, Veh) for 5 days and Sham‐irradiation (IRR) or whole‐body 33‐GCR (0.75Gy) on the 4th day. Three‐months post‐IRR, mice underwent two touchscreen‐platform tests: (1) location discrimination reversal (tests behavior pattern separation and cognitive flexibility, abilities reliant on the dentate gyrus) and (2) stimulus–response learning/extinction. Mice then underwent arena‐based behavior tests (e.g. open field, 3‐chamber social interaction). At the experiment's end (14.25‐month post‐IRR), an index relevant to neurogenesis was quantified (doublecortin‐immunoreactive [DCX+] dentate gyrus immature neurons). Female mice exposed to Veh/Sham vs. Veh/33‐GCR had similar pattern separation (% correct to 1st reversal). There were two effects of diet: CDDO‐EA/Sham and CDDO‐EA/33‐GCR mice had better pattern separation vs. their respective control groups (Veh/Sham, Veh/33‐GCR), and CDDO‐EA/33‐GCR mice had better cognitive flexibility (reversal number) vs. Veh/33‐GCR mice. One radiation effect/CDDO‐EA countereffect also emerged: Veh/33‐GCR mice had slower stimulus–response learning (days to completion) vs. all other groups, including CDDO‐EA/33‐GCR mice. In general, all mice showed normal anxiety‐like behavior, exploration, and habituation to novel environments. There was also a change relevant to neurogenesis: Veh/33‐GCR mice had fewer DCX+ dentate gyrus immature neurons vs. Veh/Sham mice. Our study implies space radiation is a risk to a female crew's longitudinal mission‐relevant cognitive processes and CDDO‐EA is a potential dietary countermeasure for space‐radiation CNS risks.
image
Research Progress on the Application of Injectable Hydrogel in Oral Tissue Regeneration
Authors: Wang Hairong , Gao Biyun 
Published: September 2024

ABSTRACTBackgroundOral and maxillofacial tissue defects resulting from factors such as trauma or infection, can significantly impact both facial function and aesthetics. Additionally, the complex anatomical structure of the face often increases the difficulty of treatment. With the advantages of controlled release, targeted delivery, and enhanced mechanical properties, injectable hydrogels have been investigated for the treatment of oral and maxillofacial diseases. In the field of regeneration, injectable hydrogels have a structure similar to the extracellular matrix (ECM) and are biocompatible, which can be used as scaffolds for tissue regeneration.ObjectiveThis review aims to summarize the literature on the current status and limitations of injectable hydrogels in the field of oral tissue regeneration.MethodsWe searched Pubmed and Web of Science databases to find and summarize the articles on the application of injectable hydrogels in tissue regeneration.ConclusionsThis review focuses on the current status and limitations of injectable hydrogels in the field of tissue regeneration (periodontal tissue, dentin‐pulp complex, bone and cartilage, salivary gland regeneration, and mucosal repair). Although fully studied in animal models, there are still challenges in clinical transformation of injectable hydrogels in promoting tissue regeneration.
High glucose promotes lipopolysaccharide‐induced macrophage pyroptosis through GSDME O‐GlcNAcylation
Authors: Xu Huifeng 
Published: September 2024

AbstractAimThe high glucose (HG) environment in diabetic periodontitis aggravates the damage of periodontal tissue. Pyroptosis has been shown to be positively correlated with the severity of periodontitis, including macrophage pyroptosis. O‐GlcNAcylation is a posttranslational modification that is involved in the pathogenesis of periodontitis. However, whether HG regulates macrophage pyroptosis through O‐GlcNAcylation remains uncertain. This study aimed to investigate the effect of HG on the O‐GlcNAcylation level of a pyroptosis regulator GSDME in macrophages to further probe the mechanisms of diabetic periodontitis.MethodsBlood samples were collected from patients with diabetic periodontitis. THP‐1 monocytes were induced to differentiate into macrophages by phorbol 12‐myristate 13‐acetate and then treated with HG to simulate periodontitis in vitro. GSDME expression of blood samples and macrophages was measured by quantitative real‐time PCR. Pyroptosis was assessed by propidium iodide staining, measurement of cell viability, cytotoxicity, protein levels of inflammation factors, and pyroptosis‐related proteins. O‐GlcNAcylation of GSDME was analyzed using co‐immunoprecipitation (co‐IP), IP, and western blot.ResultsThe results showed that GSDME expression was elevated in patients with periodontitis and HG‐treated macrophages. HG inhibited cell viability but increased LDH content, levels of IL‐1β, IL‐18, TNF‐α, NLRP3, GSDMD, and Caspase‐1, indicating that HG promoted pyroptosis of macrophages, which was reversed by GSDME knockdown. HG treatment increased O‐GlcNAcylation in macrophages. Mechanically, GSDME interacted with OGT, and OGT knockdown suppressed O‐GlcNAcylation of GSDME at Ser (S)339 site. Knockdown of OGT inhibited pyroptosis in HG‐treated macrophages, while GSDME overexpression partially reversed this inhibition.ConclusionHG treatment enhanced OGT‐mediated GSDME O‐GlcNAcylation, thereby augmenting pyroptosis in LPS‐induced macrophages. These results may provide a novel sight for the treatment of periodontitis.
How do medical students' expectations shape their experiences of well‐being programmes?
Authors: Tan Emmanuel , Driessen Erik , Frambach Janneke , Cleland Jennifer , Kearney Grainne P. 
Published: September 2024

AbstractIntroductionMedical schools have a duty of care to support their students' health and well‐being. Student support studies have tended to focus on outcomes in respect of effectiveness and satisfaction. In contrast, little is known about how student expectations of support may shape their experiences and engagement with support mechanisms, as well as the relationships students have with those offering support (including the institution itself). To address this gap in knowledge, we explore how medical students' expectations of student support systems shape, and are shaped by, institutional rules and processes.MethodsWe employed a qualitative case study approach using an institutional ethnography informed methodology. Our context was a medical school that provides a well‐advertised, formal institutional support system where students are assigned a personal tutor. Data collection included interviews with medical students (n = 13) plus document analysis (public facing artefacts and internal policies/guidelines related to the support system). We applied the lens of psychological contract theory to guide abductive analysis of interview and text data.ResultsStudents expected a strong support system to be provided by the medical school and the personal tutors. However, their experiences did not always align with their expectations. Some felt excluded by the system while others regarded the relationship with their personal tutor as more transactional than relational. Where their expectations were unmet, students responded by reducing their engagement with the formal support system and creating their own peer‐support network that supplemented existing formal support.DiscussionStudent expectations matter in shaping their experiences of support systems. Where expectations are unmet, students may disengage and/or find alternatives. This may be easier for some students compared with others. More understanding of the relationship between expectations and engagement with support can inform the development of institutional support structures that meet the needs of all students across time.
Modeling Hemodynamics in Three‐Dimensional, Biomimetic, Branched, Microfluidic, Vascular Networks
Authors: Ramanathan Rahul , Borum Andy , Rooney David M. , Rabbany Sina Y. 
Published: September 2024

ABSTRACTObjectiveNeovascularization has been extensively studied because of its significant role in both physiological processes and diseases. The significance of vascular microfluidic platforms lies in its essential role in recreating an in vitro environment capable of supporting cellular and tissue systems through the process of neovascularization. Biomechanical properties in a tissue engineered system use fluid flow and transport properties to recapitulate physiological systems. This enables mimicry of organ systems which can further personalized and regenerative medicine. Thus, fluid hemodynamics can be used to study these flow patterns and create a system that mimics real physiological pathways and processes. The establishment of stable flow pathways encourages endothelial cells (ECs) ECs to undergo neovascularization. Specifically, the shear stress applied in capillary beds generates the increased proliferation and differentiation of ECs to build larger microcirculatory beds.Mathematical FrameworkHere, we describe a mathematical model that uses branching patterns and vessel morphology to predict hemodynamic parameters in capillary beds.ResultsA retinal capillary bed is used as one‐use case of our model to show how the mathematical framework can be used to determine hemodynamic parameters for any microfluidic system.ConclusionIn doing so, this tool can be altered to be used to supplement emerging research areas in neovascularization.
Severe hypertension in tuberous sclerosis complex caused by renal artery stenosis: A case report
Authors: Xiong Yi , Li Sanlin , Shen Gang 
Published: September 2024

AbstractTuberous sclerosis complex (TSC) is a rare autosomal dominant neurocutaneous disease. Arterial hypertension is one of its uncommon complications, which is supposed to be caused by renal cysts or angiomyolipomas. Few studies have been reported in the literature on renal artery stenosis (RAS) as the cause of hypertension in TSC. Hence, we reported a boy who presented with uncontrolled hypertension under five anti‐hypertension drugs and was diagnosed with TSC complicated with left RAS. His high blood pressure was relieved by percutaneous transluminal renal angioplasty (PTRA). In one and a half years follow‐up, his blood pressure was normal whilst he took four anti‐hypertensive drugs. In conclusion, children with TCS complicated with hypertension should be carefully screened for RAS, which might be relieved by percutaneous balloon dilatation.
Microbiota responses to mutations affecting NO homeostasis in Arabidopsis thaliana
Authors: Berger Antoine , Pérez‐Valera Eduardo , Blouin Manuel , Breuil Marie‐Christine , Butterbach‐Bahl Klaus , Dannenmann Michael , Besson‐Bard Angélique , Jeandroz Sylvain , Valls Josep , Spor Aymé , Subramaniam Logapragasan , Pétriacq Pierre , Wendehenne David , Philippot Laurent 
Published: September 2024

Summary

Interactions between plants and microorganisms are pivotal for plant growth and productivity. Several plant molecular mechanisms that shape these microbial communities have been identified. However, the importance of nitric oxide (NO) produced by plants for the associated microbiota remains elusive.

Using Arabidopsis thaliana isogenic mutants overproducing NO (nox1, NO overexpression) or down‐producing NO (i.e. nia1nia2 impaired in the expression of both nitrate reductases NR1/NIA1 and NR2/NIA2; the 35s::GSNOR1 line overexpressing nitrosoglutathione reductase (GSNOR) and 35s::AHB1 line overexpressing haemoglobin 1 (AHB1)), we investigated how altered NO homeostasis affects microbial communities in the rhizosphere and in the roots, soil microbial activity and soil metabolites.

We show that the rhizosphere microbiome was affected by the mutant genotypes, with the nox1 and nia1nia2 mutants causing opposite shifts in bacterial and fungal communities compared with the wild‐type (WT) Col‐0 in the rhizosphere and roots, respectively. These mutants also exhibited distinctive soil metabolite profiles than those from the other genotypes while soil microbial activity did not differ between the mutants and the WT Col‐0.

Our findings support our hypothesis that changes in NO production by plants can influence the plant microbiome composition with differential effects between fungal and bacterial communities.
Effects of online high‐definition transcranial direct current stimulation over left dorsolateral prefrontal cortex on predominant negative symptoms and EEG functional connectivity in patients with schizophrenia: a randomized, double‐blind, controlled trial
Authors: Yeh Ta‐Chuan , Lin Yen‐Yue , Tzeng Nian‐Sheng , Kao Yu‐Chen , Chung Yong‐An , Chang Chuan‐Chia , Fang Hsu‐Wei , Chang Hsin‐An 
Published: September 2024

AimsSchizophrenia, a debilitating mental disorder, is characterized by persistent negative symptoms such as avolition and anhedonia. Currently, there are no effective treatments available for these symptoms. Thus, our study aims to assess the efficacy of online high‐definition transcranial direct current stimulation (online HD‐tDCS) in addressing the negative symptoms of schizophrenia, utilizing a double‐blind, randomized, sham‐controlled trial design.MethodsFifty‐nine patients with schizophrenia were randomized to receive either active HD‐tDCS or sham stimulation, targeting the left dorsolateral prefrontal cortex. Outcomes were measured by changes in the Positive and Negative Syndrome Scale Factor Score for Negative Symptom (PANSS‐FSNS). Exact low‐resolution electromagnetic tomography was used to assess the functional connectivity.ResultsAll 59 participants, including 50.84% females with an average age of 43.36 years, completed the trial. In the intention‐to‐treat analysis, patients receiving active HD‐tDCS showed greater improvement in PANSS‐FSNS scores compared to those receiving the sham procedure. The differences were 2.34 (95% confidence interval [CI], 1.28–3.40), 4.28 (95% CI, 2.93–5.62), and 4.91 (95% CI, 3.29–6.52) after the intervention, as well as at 1‐week and 1‐month follow‐ups, respectively. A tingling sensation on the scalp was more common in the active group (63.3%) compared to the sham group (10.3%). Additionally, HD‐tDCS was associated with a decrease in delta‐band connectivity within the default mode network.ConclusionsHigh‐definition transcranial direct current stimulation was effective and safe in ameliorating negative symptoms in patients with schizophrenia when combined with online functional targeting.