bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin prevents mitochondrial dynamics disturbances in early 5/6 nephrectomy: Relation to oxidative stress and mitochondrial bioenergetics
Authors: Aparicio‐Trejo Omar Emiliano , Tapia Edilia , Molina‐Jijón Eduardo , Medina‐Campos Omar Noel , Macías‐Ruvalcaba Norma Angélica , León‐Contreras Juan Carlos , Hernández‐Pando Rogelio , García‐Arroyo Fernando E. , Cristóbal Magdalena , Sánchez‐Lozada Laura Gabriela , Pedraza‐Chaverri José 
Published: November 2016

AbstractFive‐sixths nephrectomy (5/6NX) is a widely used model to study the mechanisms leading to renal damage in chronic kidney disease (CKD). However, early alterations on renal function, mitochondrial dynamics, and oxidative stress have not been explored yet. Curcumin is an antioxidant that has shown nephroprotection in 5/6NX‐induced renal damage. The aim of this study was to explore the effect of curcumin on early mitochondrial alterations induced by 5/6NX in rats. In isolated mitochondria, 5/6NX‐induced hydrogen peroxide production was associated with decreased activity of complexes I and V, decreased activity of antioxidant enzymes, alterations in oxygen consumption and increased MDA‐protein adducts. In addition, it was found that 5/6NX shifted mitochondrial dynamics to fusion, which was evidenced by increased optic atrophy 1 and mitofusin 1 (Mfn1) and decreased fission 1 and dynamin‐related protein 1 expressions. These data were confirmed by morphological analysis and immunoelectron microscopy of Mfn‐1. All the above‐described mechanisms were prevented by curcumin. Also, it was found that curcumin prevented renal dysfunction by improving renal blood flow and the total antioxidant capacity induced by 5/6NX. Moreover, in glomeruli and proximal tubules 5/6NX‐induced superoxide anion production by uncoupled nitric oxide synthase (NOS) and nicotinamide adenine dinucleotide phosphate oxidase (NOX) dependent way, this latter was associated with increased phosphorylation of serine 304 of p47phox subunit of NOX. In conclusion, this study shows that curcumin pretreatment decreases early 5/6NX‐induced altered mitochondrial dynamics, bioenergetics, and oxidative stress, which may be associated with the preservation of renal function. © 2016 BioFactors, 43(2):293–310, 2017
Cooperation between the bacterial‐derived short‐chain fatty acid butyrate and interleukin‐22 detected in human Caco2 colon epithelial/carcinoma cells
Authors: Bachmann Malte , Meissner Carlotta , Pfeilschifter Josef , Mühl Heiko 
Published: November 2016

ABSTRACTBy generating biologically active factors luminal microbiota shape the intestinal micro‐milieu thereby regulating pathological processes such as inflammation and carcinogenesis. Preclinical data suggest that bacterial‐derived butyrate and the signal transducer and activator of transcription (STAT)‐3 activating cytokine interleukin (IL)‐22 display concordant protective properties at the inflamed colonic epithelium. Herein, biochemical cooperation between the short‐chain fatty acid butyrate and IL‐22 was investigated by focusing on human Caco2 colon epithelial/carcinoma cells. We report that physiological levels of butyrate enhance IL‐22 signaling thereby enforcing expression of the prototypic STAT3‐downstrean target genes α1‐antichymotrypsin and suppressor of cytokine signaling (SOCS)‐3. A dual mode of butyrate action on the IL‐22/STAT3 axis was identified. Butyrate acted by upregulating IL‐22R1, the decisive chain of the heterodimeric IL‐22 receptor, and, independent from that, has the potential to directly amplify STAT3‐mediated gene activation as detected by chromatin immunoprecipitation analysis of STAT3 binding to the SOCS3 promoter. Since trichostatin A acted similarly, inhibition of histone deacetylases is likely at the root of these butyrate biological properties. The mutual benefit gained from interactions between the host and commensal intestinal bacteria‐derived factors is an expanding field of research beginning to affect clinical practice. Data presented herein propose a supportive and fine‐tuning role for butyrate in IL‐22 signaling that might be therapeutically exploited by local butyrate administration or by increasing its bacterial production in the context of a fiber‐rich diet. © 2016 BioFactors, 43(2):283–292, 2017
RETRACTED: Inhibition of the SphK1/S1P signaling pathway by melatonin in mice with liver fibrosis and human hepatic stellate cells
Authors: González‐Fernández Bárbara , Sánchez Diana I. , Crespo Irene , San‐Miguel Beatriz , Álvarez Marcelino , Tuñón María J. , González‐Gallego Javier 
Published: November 2016

AbstractThe sphingosine kinase 1/sphingosine 1‐phosphate (SphK1/S1P) system is involved in different pathological processes, including fibrogenesis. Melatonin abrogates activation of hepatic stellate cells (HSCs) and attenuates different profibrogenic pathways in animal models of fibrosis, but it is unknown if protection associates with its inhibitory effect on the SphK1/S1P axis. Mice in treatment groups received carbon tetrachloride (CCl4) 5 μL g−1 body wt i.p. twice a week for 4 or 6 weeks. Melatonin was given at 5 or 10 mg kg−1 day−1 i.p, beginning 2 weeks after the start of CCl4 administration. At both 4 and 6 weeks following CCl4 treatment, liver mRNA levels, protein concentration and immunohistochemical labelling for SphK1 increased significantly. S1P production, and expression of S1P receptor (S1PR)1, S1PR3 and acid sphingomyelinase (ASMase) were significantly elevated. However, there was a decreased expression of S1PR2 and S1P lyase (S1PL). Melatonin attenuated liver fibrosis, as shown by a significant inhibition of the expression of α‐smooth muscle actin (α‐SMA), transforming growth factor (TGF)‐β and collagen (Col) Ι. Furthermore, melatonin inhibited S1P production, lowered expression of SphK1, S1PR1, SP1R3, and ASMase, and increased expression of S1PL. Melatonin induced a reversal of activated human HSCs cell line LX2, as evidenced by a reduction in α‐SMA, TGF‐β, and Col I expression. Melatonin‐treated cells also exhibited an inhibition of the SphK1/S1P axis. Antifibrogenic effect of SphK1 inhibition was confirmed by treatment of LX2 cells with PF543. Abrogation of the lipid signaling pathway by the indole reveals novel molecular pathways that may account for the protective effect of melatonin in liver fibrogenesis. © 2016 BioFactors, 43(2):272–282, 2017
Mutual benefit for foreign medical students and Chinese postgraduates: A mixed team‐based learning method overcomes communication problems in hematology clerkship
Authors: Chen Xianling , Chen Buyuan , Li Xiaofan , Song Qingxiao , Chen Yuanzhong 
Published: November 2016

AbstractHematology is difficult for students to learn. A beneficial education method for hematology clerkship training is required to help students develop clinical skills. Foreign medical students often encounter communication issues in China. To address this issue, Chinese post‐graduates from our institute are willing to assist with educating foreign students. Therefore, we propose a mixed team‐based learning method (MTBL) which might overcome communication problems in hematology clerkship. Twenty‐two foreign medical Students attended a 2‐week hematology clerkship in Fujian Medical University Union Hospital. Twenty‐one foreign African medical students were assigned randomly into two groups. Fourteen foreign African medical students were assigned to MTBL group. Each MTBL team included two foreign African medical students and one Chinese post‐graduate. Seven foreign African medical students were assigned to lecture‐based learning method (LBL) group, which had a foreign medical classmate from Hong Kong or Chinese intern volunteers to serve as translators. The practice test scores of MTBL were significantly higher than LBL group (p < 0.05). The MTBL group had increased motivation to prepare before class, an engaged classroom atmosphere, and an improvement in their understanding of difficult topics. Interestingly, the Chinese post‐graduates also benefited from this setting, as they found that this interaction improved their communication in the English language. The mixed team‐based learning method overcomes communication problems in hematology clerkship. Foreign medical students and Chinese post‐graduates alike can benefit from MTBL. © 2016 by The International Union of Biochemistry and Molecular Biology, 45(2):93–96, 2017.
Influence of Hydrophilicity on the Snβ‐Catalyzed Baeyer–Villiger Oxidation of Cyclohexanone with Aqueous Hydrogen Peroxide
Authors: Conrad Sabrina , Wolf Patrick , Müller Philipp , Orsted Hailey , Hermans Ive 
Published: November 2016

AbstractSnβ zeolites are amongst the most effective heterogeneous catalysts for the Baeyer–Villiger (BV) oxidation of ketones with aqueous hydrogen peroxide (H2O2). The high selectivity is rooted in the activation of the carbonyl substrate through interaction with the isolated SnIV sites. However, these sites are also accessible to other molecules in the reaction mixture (in particular the co‐solvent and product). In this contribution, we report the impact of competitive adsorption on the Snβ‐catalyzed BV oxidation of cyclohexanone with aqueous H2O2. We furthermore prepared a series of Snβ zeolites with varying amounts of framework silanols and quantified their hydrophilicities with water adsorption and IR experiments. By correlating the results with catalytic data, we show that Snβ zeolites with an intermediate hydrophilicity achieve the highest activity. Our adaptable post‐synthetic synthesis allows us to tune the material synthesis, resulting in enhanced activity compared with conventional hydrothermal Snβ.
Nickel‐Catalyzed N‐Arylation of Primary Amides and Lactams with Activated (Hetero)aryl Electrophiles
Authors: Lavoie Christopher M. , MacQueen Preston M. , Stradiotto Mark 
Published: November 2016

AbstractThe first nickel‐catalyzed N‐arylation of amides with (hetero)aryl (pseudo)halides is reported, enabled by use of the air‐stable pre‐catalyst (PAd‐DalPhos)Ni(o‐tolyl)Cl (C1). A range of structurally diverse primary amides and lactams were cross‐coupled successfully with activated (hetero)aryl chloride, bromide, triflate, tosylate, mesylate, and sulfamate electrophiles.
Mass Spectrometry‐Based Bottom‐Up Proteomics: Sample Preparation, LC‐MS/MS Analysis, and Database Query Strategies
Authors: Wither Matthew J. , Hansen Kirk C. , Reisz Julie A. 
Published: November 2016

AbstractRecent technological advances in mass spectrometry (MS) have made possible the investigation and quantification of complex mixtures of biomolecules. The exceptional sensitivity and resolving power of today's mass spectrometers allow for the detection of proteins and peptides at low femtomole quantities; however, these attributes demand high sample purity to minimize artifacts and achieve the highest degree of biomolecule identification. Tissue preparation for proteomic studies is particularly challenging due to their heterogeneity in cell type, presence of insoluble biomaterials, and wide diversity of biomolecules. The workflow described herein details sample preparation from tissues through protein extraction, proteolysis, and purification to generate peptides for MS analysis. Increased peptide resolution and a corresponding increase in protein identification is accomplished using polarity‐based fractionation (C18 resin) at the peptide level. Additionally, approaches to instrument set up, including the use of nanoscale liquid chromatography and quadrupole Orbitrap MS, along with database searching, are described. © 2016 by John Wiley & Sons, Inc.
Comparative Protein Structure Modeling Using MODELLER
Authors: Webb Benjamin , Sali Andrej 
Published: November 2016

AbstractComparative protein structure modeling predicts the three‐dimensional structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). The prediction process consists of fold assignment, target‐template alignment, model building, and model evaluation. This unit describes how to calculate comparative models using the program MODELLER and how to use the ModBase database of such models, and discusses all four steps of comparative modeling, frequently observed errors, and some applications. Modeling lactate dehydrogenase from Trichomonas vaginalis (TvLDH) is described as an example. The download and installation of the MODELLER software is also described. © 2016 by John Wiley & Sons, Inc.
Basic Techniques in Mammalian Cell Tissue Culture
Authors: Phelan Katy , May Kristin M. 
Published: November 2016

AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.
Development of a Hybrid Simulator for Power System Analysis for a High Penetration of Distributed Generations
Authors: KITAGAWA RYOUHEI , TAKAGI TERUO , YOKOI KOICHI , SHIMOMURA KIMIHIKO , HARADA ATSUSHI , TAKANO SACHIO , SUZUKI MOTOJI , KOJIMA TAKEHIKO , SHIMADA SEIICHIROU , HANZAKI YOSHISHIGE 
Published: November 2016

SUMMARYChubu Electric Power Co., Ltd.’s hybrid power system simulator consists of analog models and hybrid models. The power system facilities are downsized as an analog model, while the synchronous generators and the loads are modeled as an actual current source model through the amplifier and the digital model. In recent years, there is a growing interest in analyzing power system dynamic phenomena caused by a high penetration of distributed generations. Therefore, the multifunctional generator model that can simulate distributed generation has been developed.
Performance Evaluation of Lithium‐Ion Batteries on Small Electric Bus
Authors: HORITA TSUYOSHI , SHIMAZAKI JUNPEI 
Published: November 2016

SUMMARYWe evaluate performance of lithium‐ion batteries on the small electric bus, conducting tests of cell and battery pack using discharge/charge machine. We suggest the test item on distinction between good and bad of a battery. In the discharge/charge cycle tests of cell at environmental temperature (25 °C), the relative capacity was 60% at 10,000 cycles. In the discharge capacity test of battery packs on the small electric bus, the relative capacity maintained more than 90% in progress for approximately 900 days. Finally, based on these results, we analyzed about influence factor on a battery discharge capacity.
A Verification of Estimation Accuracy of Lightning Current Waveform and Charge Transfer from Measured E‐Field Waveform
Authors: KURIHARA SATOSHI , NAKATA HIDEHIRO , HASHIMOTO YOSUKE , MICHISHITA KOJI 
Published: November 2016

SUMMARYTo establish effective countermeasures on power facilities against lightning damage, it is necessary to clarify the lightning characteristics such as current parameters at the area of interest. In order to collect lightning current parameters effectively, the authors have observed electric and magnetic field waveforms simultaneously at south Kyushu, where the lightning flash density in summer is high. Based on observed electric field waveforms, the lightning current parameters have been estimated by adopting the Diendorfer–Uman model (DU model) as the return‐stroke model. In this paper, the accuracy of the estimated return‐stroke current waveform is verified by comparison with directly measured return‐stroke current waveform, along with the accuracy of the estimated charge transfer from the electric field waveform measured with a slow antenna.
Protein‐based nanoformulations for α‐tocopherol encapsulation
Authors: Ghaheh Fatemeh Shahmoradi , Khoddami Akbar , Alihosseini Farzaneh , Gomes Andreia , Ribeiro Artur , Cavaco‐Paulo Artur , Silva Carla 
Published: November 2016

Nanoparticles of BSA and silk fibroin (SF) with entrapped α‐tocopherol were produced via ultrasonic emulsification. Populations with particle size of 200–300 nm and highly negatively charged were obtained for all the tested formulations. Entrapment efficiencies of around 99% revealed the effective encapsulation of α‐tocopherol into the produced nanoformulations. Generally, these nanodevices did not induce significant cytotoxicity to human skin keratinocytes for all the concentrations tested. The developed formulations showed free radical scavenging of ABTS.+ ability resulting from the synergistic effect between the proteins in formulation and the entrapped tocopherol. Overall, the results contribute for the establishment of BSA:VO and BSA:SF:VO as biodegradable and non‐toxic nanoformulations for the functionalization of textile devices and controlled delivery of tocopherol into the skin.
Aberrant cytokine secretion and zinc uptake in chronic cadmium‐exposed lung epithelial cells
Authors: Xu Yan‐Ming , Gao Yang‐Min , Wu Dan‐Dan , Yu Fei‐Yuan , Zang Zhong‐Sheng , Yang Lei , Yao Yue , Cai Na‐Li , Zhou Yuan , Chiu Jen‐Fu , Ching Yick‐Pang , Lau Andy T. Y. 
Published: November 2016

PurposeOur previous results showed that cadmium (Cd)‐adapted lung epithelial cells (LECs) developed resistance to apoptosis due to non‐responsiveness of the c‐Jun N‐terminal kinase pathway and augmented expression of cytokeratin 8. Since cellular Cd entry is a prerequisite in order for Cd to elicit its cytotoxicity, therefore, we wonder if there are differential metal ion transport ability and also other phenotypic changes that occurred in these Cd‐resistant LECs.Experimental design and resultsHere, we explored further and found that the zinc (Zn) importer Zip8 was stably abolished in these cells along with a marked decrease of Cd and Zn accumulation. Moreover, by cell migration assays and cytokine antibody array analysis, we found that Cd‐adapted cells exhibit enhanced migratory ability possibly due to elevated secretions of vascular endothelial growth factor and macrophage inflammatory protein‐3 alpha (MIP‐3α).Conclusion and clinical relevanceTaken together, our results show that during chronic Cd exposure, lung cells antagonize excessive cellular Cd‐influx by abolishing Zip8 expression to reduce Cd‐toxicity; however, this also renders cells with a diminished Zn uptake. The imbalance of Zn homeostasis and elevation of angiogenic and epithelial–mesenchymal transition‐promoting cytokines in Cd‐adapted cells might thus likely promote Zn deficiency, angiogenesis, and cell invasion.
Exposure to tobacco smoke in utero or during early childhood and risk of hypomania: Prospective birth cohort study
Authors: Mackay D.F. , Anderson J.J. , Pell J.P. , Zammit S. , Smith D.J. 
Published: November 2016

AbstractObjectivesUsing data from a prospective birth cohort, we aimed to test for an association between exposure to tobacco smoke in utero or during early development and the experience of hypomania assessed in young adulthood.MethodsWe used data on 2957 participants from a large birth cohort (Avon longitudinal study of parents and children [ALSPAC]). The primary outcome of interest was hypomania, and the secondary outcome was “hypomania plus previous psychotic experiences (PE)”. Maternally-reported smoking during pregnancy, paternal smoking and exposure to environmental tobacco smoke (ETS) in childhood were the exposures of interest. Multivariable logistic regression was used and estimates of association were adjusted for socio-economic, lifestyle and obstetric factors.ResultsThere was weak evidence of an association between exposure to maternal smoking in utero and lifetime hypomania. However, there was a strong association of maternal smoking during pregnancy within the sub-group of individuals with hypomania who had also experienced psychotic symptoms (OR = 3.45; 95% CI: 1.49–7.98;P= 0.004). There was no association between paternal smoking, or exposure to ETS during childhood, and hypomania outcomes.ConclusionsExposure to smoking in utero may be a risk factor for more severe forms of psychopathology on the mood-psychosis spectrum, rather than DSM-defined bipolar disorder.
Cognitive behavioral therapy for compulsive buying behavior: Predictors of treatment outcome
Authors: Granero R. , Fernández-Aranda F. , Mestre-Bach G. , Steward T. , Baño M. , Agüera Z. , Mallorquí-Bagué N. , Aymamí N. , Gómez-Peña M. , Sancho M. , Sánchez I. , Menchón J.M. , Martín-Romera V. , Jiménez-Murcia S. 
Published: November 2016

AbstractBackgroundCompulsive buying behavior (CBB) is receiving increasing consideration in both consumer and psychiatric-epidemiological research, yet empirical evidence on treatment interventions is scarce and mostly from small homogeneous clinical samples.ObjectivesTo estimate the short-term effectiveness of a standardized, individual cognitive behavioral therapy intervention (CBT) in a sample of n = 97 treatment-seeking patients diagnosed with CBB, and to identify the most relevant predictors of therapy outcome.MethodThe intervention consisted of 12 individual CBT weekly sessions, lasting approximately 45 minutes each. Data on patients’ personality traits, psychopathology, sociodemographic factors, and compulsive buying behavior were used in our analysis.ResultsThe risk (cumulative incidence) of poor adherence to the CBT program was 27.8%. The presence of relapses during the CBT program was 47.4% and the dropout rate was 46.4%. Significant predictors of poor therapy adherence were being male, high levels of depression and obsessive-compulsive symptoms, low anxiety levels, high persistence, high harm avoidance and low self-transcendence.ConclusionCognitive behavioral models show promise in treating CBB, however future interventions for CBB should be designed via a multidimensional approach in which patients’ sex, comorbid symptom levels and the personality-trait profiles play a central role.
Unilateral EEG sweat artifacts in a patient of brainstem hemorrhage with central Horner's syndrome
Authors: Singh Pankaj , Bansal A. 
Published: November 2016

AbstractWe present an interesting EEG finding of persistent unilateral sweat artifacts in a 47 years old male patient with right hemiplegia secondary to left thalamic bleed with extension of hemorrhage into the left internal capsule, the left midbrain and ventricles. The EEG was done for altered sensorium. It showed unilateral (right sided) sweat artifacts throughout the EEG recording. The anhydrosis on left side of the face was due to left sided Horner's syndrome because of involvement of first order neurons in the left midbrain. This EEG finding is highlighted because of it being relatively less common but interesting finding.