bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA‐802 plays a tumour suppressive role in tongue squamous cell carcinoma through directly targeting MAP2K4
Authors: Wu Xiaozhen , Gong Zuode , Sun Lanying , Ma Long , Wang Qibao 
Published: March 2017

AbstractObjectivesTongue squamous cell carcinoma (TSCC) is the most common oral tumours. MicroRNAs play crucial roles in many cell processes including cell viability, development, apoptosis, migration and invasion. The role of miR‐802 in the TSCC is still unknown.Materials and methodsThe miR‐802 expression in TSCC tissues and cell lines was determined by quantitative real‐time polymerase chain reaction. CCK‐8 assay was performed to measure the cell viability, while the cell invasion assay was used to determine the cell invasion. Dual‐luciferase reporter and western blot were used to confirm the potential target gene of miR‐802.ResultsIn our study, we demonstrated that miR‐802 expression was downregulated in TSCC tissues and cell lines. Elevated expression of miR‐802 suppressed the TSCC cell viability and invasion. Moreover, enforced expression of miR‐802 increased the expression of E‐cadherin, while suppressed the expression of N‐cadherin, Snail and Vimentin in the TSCC cell. In addition, we identified the mitogen‐activated protein kinase 4 (MAP2K4) as a direct target gene of miR‐802 in the TSCC cell. We also demonstrated that the expression of MAP2K4 was higher in the TSCC tissues than that in the adjacent normal tissues. Furthermore, the expression level of MAP2K4 was inversely associated with the expression of miR‐802 in TSCC tissues. We also demonstrated that the MAP2K4 expression was upregulated in TSCC cell lines. Elevated expression of miR‐802 inhibited TSCC cell viability and invasion through inhibiting MAP2K4 expression.ConclusionsOur data revealed that miR‐802 played as a tumour suppressor gene and might act as a therapeutic target in TSCC patients.
Self‐limiting non‐medical pharmaceutical opioid use among young people in Sydney, Australia: An exploratory study
Authors: Dertadian George C. , Dixon Thomas C. , Iversen Jennifer , Maher Lisa 
Published: March 2017

AbstractIntroduction and AimsIndicators suggest an escalation in opioid use globally, with recent HIV outbreaks linked to non‐medical pharmaceutical opioid (NMPO) use. Little is known about how young Australians engage in NMPO use.Design and MethodsDuring 2015, we conducted qualitative interviews with young people (16–29 years) who reported oral NMPO use at least twice in the past 90 days. The study included a sample of injecting (n = 14) and oral (n = 22) users. This paper focuses on the oral user group.ResultsMost participants grew up in affluent areas of Sydney, reported few health problems, rarely accessed health or welfare services and had limited contact with police. NMPO use was part of a repertoire of drug use involving: (i) use to come down from stimulants; (ii) use in conjunction with cannabis and alcohol; and (iii) use by itself. Participants reported limiting their use in order to avoid stigma and dependence.Discussion and ConclusionsResults suggest culture of self‐limiting NMPO use characterised by stable housing, disposable income and intimate friendship networks. Despite the adverse health outcomes observed in other settings, our data suggest that the patterns and meanings of NMPO use observed in this small sample may help explain the limited nature of problematic use among young Australians to date. Findings indicate a need for epidemiological data, including longitudinal data, to assess possible demographic shifts in NMPO use in Australia, and to address risk factors for dependence and transitions to injecting and heroin use in this population. [Dertadian GC, Dixon TC, Iversen J, Maher L. Self‐limiting non‐medical pharmaceutical opioid use among young people in Sydney, Australia: An exploratory study. Drug Alcohol Rev 2017;00:000‐000]
A prospective study of the substance use and mental health outcomes of young adult former and current cannabis users
Authors: Silins Edmund , Swift Wendy , Slade Tim , Toson Barbara , Rodgers Bryan , Hutchinson Delyse M. 
Published: March 2017

AbstractIntroduction and AimsThe extent to which young adult former cannabis users fare better than infrequent users is unclear. We investigated the association between cannabis use status at age 23 and substance use and mental health outcomes at age 27.Design and MethodsData were from the 20+ year cohort of the PATH Through Life Study. Lifetime cannabis users (n = 1410) at age 23 were classified as former/occasional/regular users. Multivariable logistic regression was used to estimate the association between cannabis use status at age 23 and six outcomes assessed at age 27.ResultsCompared with occasional cannabis users: (i) former users had odds of subsequent tobacco use [odds ratio (OR) = 0.67, 95% confidence interval (CI) 0.52–0.85], illicit drug use (cannabis, OR = 0.22, 95% CI 0.17–0.28; other illicit drugs, OR = 0.29, 95% CI 0.22–0.39) and mental health impairment (OR = 0.71, 95% CI 0.55–0.92) that were 29–78% lower; and (ii) regular users had odds of subsequent frequent alcohol use (OR = 2.34, 95% CI 0.67–1.34), tobacco use (OR = 3.67, 95% CI 2.54–5.30), cannabis use (OR = 11.73, 95% CI 6.81–20.21) and dependence symptoms (OR = 12.60, 95% CI 8.38–18.94), and other illicit drug use (OR = 2.95, 95% CI 2.07–4.21) that were 2–13 times greater. Associations attenuated after covariate adjustment, and most remained significant.Discussion and ConclusionsClear associations exist between cannabis use status in young adulthood and subsequent mental health and substance use. While early intervention remains important to prevent regular cannabis use and the associated harms, experimentation with cannabis use in the years leading into young adulthood may not necessarily determine an immutable pathway to mental health problems and illicit substance use. [Silins E, Swift W, Slade T, Toson B, Rodgers B, Hutchinson DM. A prospective study of the substance use and mental health outcomes of young adult former and current cannabis users. Drug Alcohol Rev 2017;00:000‐000]
The efficacy of interventions to increase physical activity participation of children with cerebral palsy: a systematic review and meta‐analysis
Authors: Reedman Sarah , Boyd Roslyn N , Sakzewski Leanne 
Published: March 2017

AimTo determine efficacy of therapy and behaviour change interventions to increase the level of participation in leisure‐time physical activities (LTPAs) and habitual physical activity in children and young people with cerebral palsy.MethodFive databases were systematically searched. Included studies were randomized or comparison designs. Methodological quality was assessed with a modified Downs and Black Scale. Quantitative analysis was performed using RevMan 5.3 (The Nordic Cochrane Centre, The Cochrane Collaboration, Copenhagen, Denmark). Intervention components and behaviour change constructs were mapped against (1) the International Classification of Functioning, Disability and Health (ICF) and (2) the Theoretical Domains Framework.ResultsSearches yielded 2487 unique articles. Eight studies (nine articles) were included. Interventions included physical training, activity level training, combined physical training and behaviour change therapy, online behaviour change modules, and context‐focused therapy. Study quality varied from moderate to high. There was a small, significant effect of physical activity intervention compared with passive usual care on level of habitual physical activity, of approximately 1000 additional steps per day (standardized mean difference 0.34, 95% confidence interval 0.03–0.66, p=0.030). There was no significant effect on LTPA participation (standardized mean difference 0.40, 95% confidence interval −0.40 to 1.19, p=0.330).InterpretationTherapy and behaviour change interventions have the potential to increase LTPA participation of children and young people with cerebral palsy, although there is a need to depart from impairment‐focused approaches. Inappropriate selection of outcomes and inadequate reporting of complex interventions are barriers to progress in this field.
Decreased insulin‐stimulated brown adipose tissue glucose uptake after short‐term exercise training in healthy middle‐aged men
Authors: Motiani Piryanka , Virtanen Kirsi A. , Motiani Kumail K. , Eskelinen Joonas J. , Middelbeek Roeland J. , Goodyear Laurie J. , Savolainen Anna M. , Kemppainen Jukka , Jensen Jørgen , Din Mueez U. , Saunavaara Virva , Parkkola Riitta , Löyttyniemi Eliisa , Knuuti Juhani , Nuutila Pirjo , Kalliokoski Kari K. , Hannukainen Jarna C. 
Published: March 2017

AimsTo test the hypothesis that high‐intensity interval training (HIIT) and moderate‐intensity continuous training (MICT) improve brown adipose tissue (BAT) insulin sensitivity.Participants and methodsHealthy middle‐aged men (n = 18, age 47 years [95% confidence interval {CI} 49, 43], body mass index 25.3 kg/m2 [95% CI 24.1‐26.3], peak oxygen uptake (VO2peak) 34.8 mL/kg/min [95% CI 32.1, 37.4] ) were recruited and randomized into six HIIT or MICT sessions within 2 weeks. Insulin‐stimulated glucose uptake was measured using 2‐[18F]flouro‐2‐deoxy‐D‐glucose positron‐emission tomography in BAT, skeletal muscle, and abdominal and femoral subcutaneous and visceral white adipose tissue (WAT) depots before and after the training interventions.ResultsTraining improved VO2peak (P = .0005), insulin‐stimulated glucose uptake into the quadriceps femoris muscle (P = .0009) and femoral subcutaneous WAT (P = .02) but not into BAT, with no difference between the training modes. Using pre‐intervention BAT glucose uptake, we next stratified subjects into high BAT (>2.9 µmol/100 g/min; n = 6) or low BAT (<2.9 µmol/100 g/min; n = 12) groups. Interestingly, training decreased insulin‐stimulated BAT glucose uptake in the high BAT group (4.0 [2.8, 5.5] vs 2.5 [1.7, 3.6]; training*BAT, P = .02), whereas there was no effect of training in the low BAT group (1.5 [1.2, 1.9] vs 1.6 [1.2, 2.0] µmol/100 g/min). Participants in the high BAT group had lower levels of inflammatory markers compared with those in the low BAT group.ConclusionsParticipants with functionally active BAT have an improved metabolic profile compared with those with low BAT activity. Short‐term exercise training decreased insulin‐stimulated BAT glucose uptake in participants with active BAT, suggesting that training does not work as a potent stimulus for BAT activation.
Engaging decentralization in an uncertain political context: Lessons from Liberia
Authors: Krawczyk Kelly , Muhula Raymond 
Published: March 2017

AbstractThis study uses a Diagnostic Framework for Local Governance to evaluate the current capacity for administrative decentralization at the county level in Liberia, as well as to identify political economy challenges and opportunities surrounding decentralization. Applying the Problem‐Driven Iterative Adaptation (PDIA) framework to our findings, we offer recommendations for moving decentralization reform forward. We find strong demand for administrative decentralization among local officials, and propose that decentralization reform can be enhanced through incremental implementation that focuses on strengthening the existing structures and capacity of local administration, and through the creation of stronger accountability and participatory mechanisms. The triangulated research design includes focus group and interview data in eight Liberian counties, analyzed using NVivo Qualitative Data Analysis Software.
Vena contracta analysis by color Doppler three‐dimensional transesophageal echocardiography shows geometrical differences between prolapse and pseudoprolapse in eccentric mitral regurgitation
Authors: Berdejo Javier , Shiota Maiko , Mihara Hirotsugu , Itabashi Yuji , Utsunomiya Hiroto , Shiota Takahiro 
Published: March 2017

AimsEvaluation of eccentric mitral regurgitation (MR) remains extremely difficult and the role played by its etiology, functional or degenerative, is not well understood. This study aimed to demonstrate the value of three‐dimensional transesophageal echocardiography (3DTEE) in the evaluation of eccentric MR identifying geometric differences in the vena contracta area between functional and degenerative etiologies.Methods and ResultsWe studied 61 patients with eccentric MR (30 functional and 31 degenerative). Regurgitant orifice area was determined by the two‐dimensional proximal isovelocity surface area (2DPISA) and the 3DTEE methods. The ratio between maximum and minimum lengths of the vena contracta was calculated in each patient. Effective regurgitant orifice area by the 2DPISA method was smaller than that estimated by 3DTEE (0.56±0.21 vs 0.72±0.25 cm2). A better correlation between both methods was seen in degenerative mitral regurgitation (DMR; r=.83), with a mean underestimation of 8.2% by the 2DPISA method. A much worse correlation was found in functional mitral regurgitation (FMR; r=.39), where a mean underestimation by the 2DPISA method of 29.1% was observed. There was a more elongated and curved vena contracta in FMR compared to that in DMR (length ratio: 3.4±1.0 vs 2.2±0.7, P<.0001).ConclusionThree‐dimensional transesophageal echocardiography identifies a more elongated regurgitant orifice in eccentric FMR compared to that in eccentric DMR. This difference may explain the greater underestimation of effective regurgitant orifice area by the 2DPISA method in FMR. High‐quality 3DTEE analysis of vena contracta area would be a highly recommended alternative.
Evaluation of the effect of dipping pattern in hypertensive patients on the left ventricular systolic functions by two‐dimensional strain analysis
Authors: Göksülük Hüseyin , Habibova Ulvin , Ongun Aydan , Akbulut Müge , Özyüncü Nil , Kürklü Türkan Seda Tan , Erol Cetin 
Published: March 2017

BackgroundNondipping blood pressure pattern carry a high risk of cardiovascular and cerebrovascular complications due to a higher cumulative pressure overload. We aimed to define the role of strain analysis for detecting subclinical left ventricular systolic dysfunction in recently diagnosed nondipper and dipper hypertensive patients with normal left ventricular systolic function.MethodsStudy population consisted of two groups of patients, Group 1: 45 dipper patients and Group 2: 43 nondipper patients. Global and segmental two‐dimensional longitudinal strain analysis were measured by speckle tracking method.ResultsThe analysis of two‐dimensional left ventricular global longitudinal strain and strain rates showed that there was a significant difference between groups (−18.1%±3.1% for nondippers vs −20.5%±2.4% for dippers, P<.001 for global longitudinal strain and −1.2±0.2 1/s for nondippers vs −1.31±0.16 1/s for dippers, P<.001 for global longitudinal strain rate). The nighttime systolic, diastolic, and mean blood pressure measurements were significantly higher in the nondipper group. Nocturnal dipping rates were statistically different between the groups (P<.001). Interventricular septum, posterior wall thickness, relative wall thickness, left atrial dimension, left ventricular mass, and mass index were higher in the nondipper group. Multivariate analysis demonstrated left atrium size, nocturnal dipping rate, daytime mean blood pressure, and nighttime systolic, diastolic, and mean blood pressure as independent predictors of global longitudinal strain.ConclusionIn our study, two‐dimensional speckle tracking examination showed that the left ventricular systolic function is impaired even in the subclinical period in recently diagnosed nondipper hypertensive patients with deformational analysis.
The role of advanced echocardiography and cardiovascular magnetic resonance in the assessment of myocardial function in Marfan syndrome—An update
Authors: Kiotsekoglou Anatoli , Moggridge James C. , Child Anne H. , Rask Peter 
Published: March 2017

Cardiovascular assessment of patients with Marfan syndrome has normally focused on the aortic root and vascular manifestations of the disease due to the high risk of aortic dissection. Although primary myocardial impairment has long been suspected in these patients, the evidence has been controversial. Advanced echocardiography and cardiovascular magnetic resonance imaging have proven to be effective, accurate, and more sensitive in the detection of subtle cardiac dysfunction. The application of these techniques to Marfan syndrome over the last 10 years has made significant progress in demonstrating the presence of primary myocardial impairment in these patients, but further work is still required to obtain confirmatory molecular, pathophysiological, and prognostic clinical data. Phenotypic expression of the disease has prognostic value, also suggesting potential effective medical therapy.
The value of diastolic flow reversal in the descending thoracic aorta as a determinant of invasively measured aortic pulse pressure
Authors: Kim Hack‐Lyoung , Kim Yu Nui , Kim Hee‐Jun , Lim Woo‐Hyun , Seo Jae‐Bin , Kim Sang‐Hyun , Kim Myung‐A , Zo Joo‐Hee 
Published: March 2017

AimAlthough the diastolic flow reversal of the descending aorta has been recognized in patients with aortic regurgitation, its generation without this condition is still unknown. This study was performed to investigate whether flow patterns of the descending thoracic aorta, as measured by echocardiography, can represent invasively measured aortic pulse pressure (APP).MethodsA total of 100 patients (age, 62.3±11.0 years; men, 62.0%) undergoing invasive coronary angiography (ICA) was analyzed. APP was measured at ascending thoracic aorta using pigtail catheter before ICA. Flow in the descending thoracic aorta was assessed using pulse wave Doppler echocardiography, and R/F ratio was defined as reverse peak velocity (R)/forward peak velocity (F).ResultsEighty patients (80.0%) had obstructive coronary artery disease (CAD) (≥50% stenosis of one or more epicardial coronary arteries) in ICA. APP and R/F ratio were significantly higher in patients with obstructive CAD than those without (P<.05 for each). Both R/F ratio (β=0.379, P<.001) and APP (β=0.255, P<.001) were positively correlated with age. In simple linear regression analysis, there was a significant positive correlation between R/F ratio and APP (β=0.266, P<.001). This correlation remained significant even after controlling for potential confounders including age, gender, E/eʹ, and left atrial volume index in multiple linear regression analysis (β=0.193, P=.036).ConclusionsR/F ratio may be independently associated with APP in patients undergoing ICA.
What is the role of innovation in productivity growth in Central and Eastern European countries?
Authors: Tevdovski Dragan , Tosevska‐Trpcevska Katerina , Disoska Elena Makrevska 
Published: March 2017

AbstractThe paper uses company level data from the Eurostat's Community Innovation Survey 2008 and applies CDM model in order to estimate the links between R&D engagement, R&D intensity, innovation output and productivity in selected Central and Eastern European Countries – Bulgaria and Romania, and compares their performance with Germany. The results showed that different processes drive company's decision to engage in R&D in Bulgaria and Romania in comparison to Germany; R&D intensity is an important factor of product innovation in the observed CEE countries and product innovation leads to higher productivity in Bulgaria and Romania, while process innovation leads to higher productivity in Bulgaria, but not in Romania.
Impact of mild to severe hemophilia on education and work by US men, women, and caregivers of children with hemophilia B: The Bridging Hemophilia B Experiences, Results and Opportunities into Solutions (B‐HERO‐S) study
Authors: Cutter Susan , Molter Don , Dunn Spencer , Hunter Susan , Peltier Skye , Haugstad Kimberly , Frick Neil , Holot Natalia , Cooper David L. 
Published: March 2017

AbstractThe psychosocial impact of hemophilia on work was recently investigated in the Hemophilia Experiences, Results and Opportunities (HERO) study. The findings revealed that hemophilia had an impact for adults with moderate/severe hemophilia and caregivers of children with hemophilia. HERO did not specifically evaluate impact on education in adults/children with mild/moderate hemophilia or the impact on employment of spouses/partners of caregivers of affected children. The Bridging Hemophilia B Experiences, Results and Opportunities into Solutions (B‐HERO‐S) study evaluated the impact of hemophilia on the lives of adult men/women with mild‐severe hemophilia B and caregivers of boys/girls with hemophilia B and their spouses/partners. Many adults with hemophilia B (94%) reported that hemophilia had a negative effect on their ability to complete a formal education, often attributed to the inability to attend or concentrate in school as a result of hemophilia‐related bleeding or pain. Most adults with hemophilia B (95%) and caregivers/partners (89%/84%) indicated that hemophilia had a negative impact on employment. Most adults with hemophilia were employed (81%), with construction/manufacturing (35%) as the most frequently reported industry; many worked in jobs requiring manual labor (39%). Of those unemployed, 62% never worked, and those who stopped working reported that they left the workforce due to financial issues (59%), including insurance coverage/co‐pays, or hemophilia‐related issues (55%). Nearly one‐third of caregivers voluntarily left the workforce to care for children with hemophilia. These results suggest a need to focus more effort on career counseling for adults with hemophilia B and caregivers of affected children, especially around mild/moderate hemophilia, as this population may not be as well informed regarding potential impact in school and the workplace.
Management of US men, women, and children with hemophilia and methods and demographics of the Bridging Hemophilia B Experiences, Results and Opportunities into Solutions (B‐HERO‐S) study
Authors: Buckner Tyler W. , Witkop Michelle , Guelcher Christine , Frey Mary Jane , Hunter Susan , Peltier Skye , Recht Michael , Walsh Christopher , Kessler Craig M. , Owens Wendy , Clark David B. , Frick Neil , Rice Michelle , Iyer Neeraj N. , Holot Natalia , Cooper David L. , Sidonio Robert 
Published: March 2017

AbstractThe Bridging Hemophilia B Experiences, Results and Opportunities Into Solutions (B‐HERO‐S) initiative was launched in an effort to address specific gaps in the understanding of the psychosocial impact of mild‐moderate‐severe hemophilia B. The original Hemophilia Experiences, Results and Opportunities (HERO) qualitative study evaluated the needs of people with hemophilia A or B in multiple countries; however, a majority of participants had the more common moderate‐severe hemophilia A. The B‐HERO‐S study was designed in collaboration with the hemophilia community to evaluate the needs of adults with hemophilia B and caregivers of children with hemophilia B, including affected women and caregivers of girls with hemophilia. The report presented here describes participant demographics and comorbidities, as well as treatment regimens and access to treatment. Bleeding symptoms were reported by 27% of mothers of children with hemophilia B who participated. Women were more likely than men to self‐report arthritis and depression/anxiety as comorbidities associated with hemophilia B. More adults and children with hemophilia B were on routine treatment than on on‐demand treatment, and a high percentage of adults with moderate hemophilia B received routine treatment (86%). Many adults with hemophilia B (78%) and caregivers (69%) expressed concern about access to factor in the next 5 years, and of adults with hemophilia B, women more commonly experienced issues with access to factor in the past than did men (72% vs 44%). The findings of the B‐HERO‐S study reveal potential unmet needs of some patients with mild‐moderate hemophilia B, and the results may be leveraged to inform patient outreach by hemophilia treatment centers and education initiatives.