bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Western dietary pattern is associated with elevated level of high sensitive C‐reactive protein among adolescent girls
Authors: Khayyatzadeh Sayyed Saeid , Bagherniya Mohammad , Fazeli Mostafa , Khorasanchi Zahra , Bidokhti Mina Safari , Ahmadinejad Malihe , Khoshmohabbat Somaieh , Arabpour Mahla , Afkhamizadeh Mozhgan , Ferns Gordon A. , Masoudifar Maryam , Ghayour‐Mobarhan Majid 
Published: January 2018

AbstractBackgroundSerum high sensitive C‐reactive protein (hs‐CRP), is an indicator of low‐grade inflammation, and is associated with several non‐communicable diseases. The effects of diet on inflammation have not been extensively investigated, particularly among adolescents. We aimed to examine the association between major dietary patterns and elevated serum level of hs‐CRP among Iranian adolescent girls.Materials and methodsIn this cross‐sectional study, a total of 670 adolescent girls were recruited from several schools in different areas of Mashhad and Sabzevar cities, Iran. The dietary intakes of study participants were collected using a 147‐item food frequency questionnaire. To identify major dietary patterns based on the 40 food groups, we used principal component analysis. Serum concentration of hs‐CRP was measured using commercial kits and the BT‐3000 auto‐analyser. To investigate the association between dietary patterns and elevated serum level of hs‐CRP, we used logistic regression analysis.ResultsThree specific dietary patterns were identified: (i) healthy, (ii) traditional and (iii) western dietary patterns. A significant association was found between more adherence to Western dietary pattern and elevated serum level of hs‐CRP (OR: 1.58; 95% CI: 1.02‐2.42, P‐trend = .03); these association remained significant after adjustment for potential confounders. However, there was no significant relationship between healthy and traditional dietary patterns and elevated serum level of hs‐CRP.ConclusionOur results indicate that there is a significant positive association between more adherence to Western dietary pattern and higher serum levels of hs‐CRP among Iranian adolescent girls. Further studies, particularly longitudinal intervention studies may be required to clarify these relationships.
Expression pattern of PD‐L1 and PD‐L2 in classical Hodgkin lymphoma, primary mediastinal large B‐cell lymphoma, and gray zone lymphoma
Authors: Tanaka Yosuke , Maeshima Akiko Miyagi , Nomoto Junko , Makita Shinichi , Fukuhara Suguru , Munakata Wataru , Maruyama Dai , Tobinai Kensei , Kobayashi Yukio 
Published: January 2018

AbstractObjectivesWe aimed at investigating the relationship between classical Hodgkin lymphoma (cHL), primary mediastinal large B‐cell lymphoma (PMBL), and gray zone lymphoma (GZL) with intermediate characteristics between cHL and PMBL, from the perspective of the aberration in programed cell death 1 and the programed death ligands (PDLs) network.MethodsWe explored the expression levels of PDLs and chromosomal anomalies in 67 cases: 34 cases with cHL, 20 with PMBL, and 13 with GZL, using immunohistochemical analyses and Fluorescence In Situ Hybridization (FISH).ResultsTwenty‐one cHL (62%), 3 PMBL (15%), and 6 GZL (46%) cases showed staining to PD‐L1 antibodies in more than 70% of tumor cells. Two cHL (6%), 10 PMBL (50%), and 3 GZL (23%) cases were not stained by PD‐L1 antibodies. Patients over 40 years old manifest more frequent expression of PD‐L1 in cHL. Proportion of tumors stained by PD‐L2 antibody was increased in PMBL. FISH analyses with a PD‐L1/PD‐L2 probe detected 5 amplification, 1 gain, and 7 polysomy cases in cHL, 1 amplification and 1 polysomy case in GZL, and amplification in 1 PMBL case.ConclusionWe identified increased staining of PD‐L1 in cHL and that of PD‐L2 in PMBL. GZL had a pattern similar to that of cHL.
Zebrafish larvae as a model to demonstrate secondary iron overload
Authors: Nasrallah Gheyath K. , Younes Nadin N. , Baji Missbah H. , Shraim Amjad M. , Mustafa Ibrahim 
Published: January 2018

AbstractObjectivesThalassemia is the most common genetically inherited blood disorder arising from a defect in hemoglobin production, resulting in ineffective erythropoiesis and severe hemolytic anemia. While transfusion therapy corrects the anemia, it gives rise to secondary iron overload. Current iron chelation therapy performed using deferoxamine, and the efficiency of this drug was demonstrated here using the zebrafish animal model.MethodsZebrafish larvae were exposed for 3 days to iron [100 μmol L−1ferric ammonium citrate; 3‐6 days post fertilization (dpf)]. Then, iron treated larvae were exposed to 100 μmol L−1deferoxamine for 3 days (6‐9 dpf). Total tissue iron concentration in the whole larvae, assessed by three different assays; inductively coupled plasma mass spectrometry, colorimetry (spectrophotometry), and microscopy using iron staining followed by imaging and quantification.ResultsThe three assays showed that iron treatment alone resulted in a significant increase in total iron. Deferoxamine treatment of the iron‐loaded zebrafish larvae showed a significant decrease in total iron concentration.ConclusionThis study presented a clear evidence of the effectiveness of zebrafish larvae to use as a tool to study iron overload and open the door for studying the efficiency of potential new iron chelating compounds other than commercially available ones.
Agmatine co‐treatment attenuates allodynia and structural abnormalities in cisplatin‐induced neuropathy in rats
Authors: Donertas Basak , Cengelli Unel Cigdem , Aydin Sule , Ulupinar Emel , Ozatik Orhan , Kaygisiz Bilgin , Yildirim Engin , Erol Kevser 
Published: January 2018

AbstractCisplatin is a widely used antineoplastic agent in the treatment of various cancers. Peripheral neuropathy is a well‐known side effect of cisplatin and has potential to result in limiting and/or reducing the dose, decreasing the quality of life. Thus, effective treatments are needed. Agmatine is an endogenous neuromodulator that has been shown to exert antiallodynic effects in various animal studies. The first aim of this study was to investigate the in vitro effects of agmatine on cisplatin‐induced neurotoxicity. Primary cultures of dorsal root ganglia (DRG) which are the primary target of drug injury were prepared. DRG cells were incubated with cisplatin (100, 200, 500 μm). Then, agmatine (10, 100, 500 μm) was administered with the submaximal concentration of cisplatin. Cisplatin caused concentration‐dependent neurotoxicity, and agmatine did not alter this effect. The second aim was to investigate the effects of agmatine on cisplatin‐induced peripheral neuropathy in rats and the influence of nitric oxide synthase (NOS) inhibitor, L‐NAME, in this effect. Female Sprague Dawley rats received intraperitoneal saline (control), cisplatin (3 mg/kg), cisplatin+agmatine (100 mg/kg), or cisplatin+agmatine+L‐NAME (10 mg/kg) once a week for 5 weeks. The mechanical allodynia, hot plate, and tail clip tests were performed, and DRG cells and sciatic nerves were analyzed. Agmatine and agmatine+L‐NAME combination attenuated CIS‐induced mechanical allodynia and degeneration in DRG cells and sciatic nerves. However, L‐NAME did not potentiate the antiallodynic or neuroprotective effect of agmatine. These findings indicate that agmatine co‐administration ameliorates cisplatin‐induced neuropathy and may be a therapeutic alternative.
Interactive effects of LPS and dentine matrix proteins on human dental pulp stem cells
Authors: Widbiller M. , Eidt A. , Wölflick M. , Lindner S. R. , Schweikl H. , Hiller K.‐A. , Buchalla W. , Galler K. M. 
Published: January 2018

AbstractAimTo investigate the combinatorial effects of lipopolysaccharide (LPS) and extracted dentine matrix proteins (eDMP) on regenerative and inflammatory responses in human dental pulp stem cells (DPSCs).MethodologyCulture media were supplemented with several concentrations of LPS, eDMP and combinations of both. Cell viability was assessed over 1 week by MTT assay; cell survival was evaluated after 24 h and 7 days by flow cytometry. The expression of mineralization‐associated marker genes was determined by real‐time quantitative polymerase chain reaction (RT‐qPCR). To analyse the inflammatory response, secretion of interleukin 6 (IL‐6) was quantified in the initial and the late phase of cell culture by enzyme‐linked immunosorbent assay (ELISA). Data were treated nonparametrically and Mann–Whitney U‐tests were performed to compare all experimental groups (α = 0.05).ResultsWhereas LPS had no impact on viability, eDMP led to a concentration‐dependent decrease, which was significant after 7 days (P ≤ 0.024). A moderate decline of cell survival induced by LPS was detected after 48 h (P ≤ 0.026), whereas eDMP was able to reverse this effect. eDMP alone caused increased expression of tested marker genes, LPS had no regulatory effect. Combined eDMP and LPS induced an upregulation of collagen type I and osteocalcin, whereas expression levels of dentine matrix acidic phosphoprotein and dentine sialophosphoprotein were similar to the control. IL‐6‐secretion was increased by LPS over time. eDMP markedly elevated initial production of IL‐6 (P ≤ 0.002), but suppressed LPS‐induced cytokine production in the later phase.ConclusionsLipopolysaccharide did not affect cell viability but interfered with odontoblast‐like cell differentiation of DPSCs. Proteins from the dentine matrix may have a protective effect, attenuate the detrimental impact of LPS and thus play an important role during pulp repair.
Systemic bone marker expression induced by grey and white mineral trioxide aggregate in normal and diabetic conditions
Authors: de Azevedo Queiroz I. O. , Mello W. G. , Martins C. M. , Dal Fabbro R. , Narciso L. G. , Massunari L. , Cintra L. T. A. , Ervolino E. , Gomes‐Filho J. E. 
Published: January 2018

AbstractAimTo investigate the relationship between diabetes mellitus and local/systemic effects of both grey and white mineral trioxide aggregate (MTA) Angelus on bone marker expression.MethodologyWistar rats were divided into two groups: healthy and diabetic (Alloxan induced), which were further divided into three subgroups (control, GMTA Angelus and WMTA Angelus). Polyethylene tubes filled with MTA materials or empty tubes were implanted in dorsal connective tissue. On days 7 and 30, blood samples were collected for calcium, phosphorus and ALP measurement. The animals were euthanized; implanted tubes were removed and processed for immunohistochemical analysis of osteocalcin (OCN) and osteopontin (OPN). Kruskal–Wallis followed by Dunn's multiple comparison test was performed for nonparametric data, and anova followed by Tukey's test for parametric data.ResultsNo difference in systemic serum calcium levels between both groups was observed. On day 7, serum phosphorus levels within the WMTA healthy group were higher than that of the diabetic group. On day 30, healthy rats exhibited lower phosphorus levels than diabetic ones. At both time points, the diabetic group was associated with more ALP activity than the healthy group. Immunohistochemical analyses of the healthy group revealed OCN‐ and OPN‐positive cells in the presence of both MTA materials. However, under diabetic conditions, both OCN and OPN were absent.ConclusionBoth MTA materials were associated with an increase in serum calcium, phosphorus and ALP, suggesting a potential systemic effect, along with triggered differentiation of OCN‐ and OPN‐positive cells. Moreover, in diabetic conditions, an inhibitory effect on MTA‐induced differentiation of OCN‐ and OPN‐positive cells was detected.
Pathogenesis and treatment options for chemotherapy‐induced alopecia: a systematic review
Authors: Rubio‐Gonzalez Belen , Juhász Margit , Fortman Jamie , Mesinkovska Natasha Atanaskova 
Published: January 2018

AbstractChemotherapy‐induced alopecia (CIA) is one of the most troubling long‐lasting side effects of cancer treatment. An estimated 65% of patients undergoing classic chemotherapy will experience hair loss, which is an extremely upsetting adverse event for many. CIA has been traditionally considered to be a diffuse, nonscarring alopecia; however, there are increasing reports of permanent hair loss post chemotherapy. Despite its large impact on patients, there are few proven treatments for CIA. Recent advancements in understanding the pathogenesis of hair loss are promising novel preventative and therapeutic strategies. Currently, scalp cooling during chemotherapy is the most effective preventive intervention with response rates ranging from 50 to 80%. To avoid patient morbidity, clinicians should be aware of the pathogenesis of CIA, characteristic patterns of hair loss associated with specific drug regimens, preventive measures that may be taken, and therapeutic options post chemotherapy. The following represents an updated systematic review of CIA, including characteristic clinical patterns, pathophysiology of the disease, therapeutic approaches, as well as a cost‐effective analysis to assess the significance of this toxicity.
Clinical features and management of schwannoma affecting the upper and lower lips
Authors: Sitenga Jenna , Aird Gregory , Vaudreuil Adam , Huerter Christopher J. 
Published: January 2018

AbstractHead and neck schwannomas comprise 25–40% of all schwannomas, with presentation on the lips as the rarest and most surgically complicated site for perioral tumors. A systematic literature review was conducted to include 21 cases of patients with schwannoma of the upper or lower lips. The majority of patients presented with a single, painless, well‐encapsulated nodule on the upper or lower lips. The nodules were consistently slow‐growing, with an average 29.3 months from symptom onset to clinical presentation. Most cases were complicated by profound cosmetic disfigurement as well as dysphagia, dysarthria, snoring, and/or sleep apnea. Overall, histological analysis was consistent with classic schwannoma, and all cases were treated via complete surgical excision, and for malignant tumors, additional therapy was utilized. All but four cases achieved full remission by final follow‐up. Recurrence rate for benign lip schwannomas was 5.3%, which is remarkably different from the standard recurrence rate of 8–24% for benign peripheral schwannomas. Additionally, the proportion of malignant tumors was greater for lip schwannomas than other schwannomas. Lip schwannomas demonstrate different characteristics than schwannomas from other locations on the body, and these remarkable differences highlight significant implications for clinical practice. Complete excision is the primary mode of treatment with overall excellent postoperative prognosis and rare instances of recurrence. Given the rarity of this tumor, this review of available cases serves to comprehensively describe clinical presentation and surgical treatment approaches to upper and lower lip schwannomas.
Monomeric phenolics in different parts of high‐acid apple (Malus sieversii f. niedzwetzkyana (Dieck) Langenf): a promising source of antioxidants for application in nutraceuticals
Authors: Lin Lianzhu , Peng An , Yang Kun , Zou Ying 
Published: January 2018

SummaryThe high‐acid apple (Malus sieversii f. niedzwetzkyana (Dieck) Langenf) possessing deep red peel and light red flesh is widely distributed in China. To determine if a useful apple variety is being ignored, the monomeric phenolic profiles and antioxidant activities of peel, flesh, pomace, whole fruit and juice were evaluated. The results were compared to those of ‘Fuji’ and ‘Granny Smith’ apples. The high‐acid apple possessing the highest total phenolic and anthocyanin contents among three apple varieties contained the most chlorogenic acid, phloridzin, epicatechin, ferulic acid, catechin, vanillic acid, p‐coumaric acid and caffeic acid. The extraction yields of phenolics (64%) and anthocyanins (89%) from the high‐acid apple to juice were the highest. The high‐acid apple possessing the greatest antioxidant activity had the strongest antihaemolysis activity, indicating that it could be used to produce juice and value‐added ingredient to assist in the prevention of chronic disease.
Cholera toxin enhances interleukin‐17A production in both CD4+ and CD8+ cells via a cAMP/protein kinase A‐mediated interleukin‐17A promoter activation
Authors: Tsai Hsing‐Chuan , Velichko Sharlene , Lee Shanshan , Wu Reen 
Published: January 2018

SummaryCholera toxin (CT) is a bacterial component that increases intracellular cAMP levels in host cells and suppresses T‐cell activation. Recently, CT was reported to induce T helper type 17‐skewing dendritic cells and activate interleukin‐17A (IL‐17A) production in CD4+ T cells through a cAMP‐dependent pathway. However, the underlying mechanism by which cAMP regulates IL‐17A production in T cells is not completely defined. In this study, we took advantage of a small molecule protein kinase A (PKA) inhibitor (H89) and different cAMP analogues: a PKA‐specific activator (N6‐benzoyl‐adenosine‐cAMP), an exchange protein activated by cAMP‐specific activator (Rp‐8‐chlorophenylthio‐2′‐O‐methyl cAMP), and a PKA inhibitor (Rp‐8‐bromo‐cAMP), to elucidate the signalling cascade of cAMP in IL‐17A regulation in T cells. We found that CT induced IL‐17A production and IL‐17A promoter activity in activated CD4+ T cells through a cAMP/PKA pathway. Moreover, this regulation was via cAMP‐response element binding protein (CREB) ‐mediated transcriptional activation by using the transfection of an IL‐17A promoter–luciferase reporter construct and CREB small interfering RNA in Jurkat cells. Also, we showed that CREB bound to the CRE motif located at −183 of the IL‐17A promoter in vitro. Most interestingly, not only in CD4+ T cells, CT also enhanced cAMP/PKA‐dependent IL‐17A production and CREB phosphorylation in CD8+ T cells. In conclusion, our data suggest that CT induces an IL‐17A‐dominated immune microenvironment through the cAMP/PKA/CREB signalling pathway. Our study also highlights the potentials of CT and cAMP in modulating T helper type 17 responses in vivo.
Achievement goals influence mastery experience via two paths in digital creativity games among elementary school students
Authors: Yeh Y.‐c. , Lin C.S. 
Published: January 2018

AbstractAlthough cultivating creativity is greatly emphasized in elementary school education and that digital games can be a promising tool for improving creativity, little research has been conducted to identify and explore how player‐related factors might influence the learning outcomes of digital creativity games. This study identifies 3 individual traits pertaining to digital creativity game playing and examines how these determinants influence self‐efficacy and mastery experiences of creativity using structural equation modelling. The participants were 275 4th through 6th graders, and the employed method was inventory investigation. The findings reveal that the participants spend a large proportion of time playing digital games after school. Moreover, the results suggest that motivation for achieving both mastery goals and performance goals is crucial to enhancing self‐efficacy and achieving mastery experience in creativity. Additionally, such motivation might enhance mastery experience via two paths: the experience of flow states and the strengthening of self‐determination and self‐efficacy. The findings of this study shed light on the design of digital games for creativity training.
The effects of computerized inquiry‐stage‐dependent argumentation assistance on elementary students' science process and argument construction skills
Authors: Lin C.‐H. , Chiu C.‐H. , Hsu C.‐C. , Wang T.‐I. , Chen C.‐H. 
Published: January 2018

AbstractThis study proposed a computerized inquiry‐stage‐dependent argumentation assistance and investigated whether this can help improve elementary students' performance in science processes and the construction of quality arguments. Various argumentation assistances were developed and incorporated into each stage of scientific inquiry in a computer‐supported scientific inquiry system. A nonequivalent quasi‐experimental design was adopted to evaluate the effectiveness of this approach. Two intact sixth grade classes (N = 55) participated in this study, and each student used a tablet computer to accomplish the designated inquiry activities. One class of students was arranged to use the stage‐dependent argumentation assistance, and the other used a generic text‐based interface. The findings indicate that students who used the stage‐dependent argumentation assistance could acquire significantly better science process and argument construction skills than those using the generic text‐based interface.
Independent mapping methods reveal rotational activation near pulmonary veins where atrial fibrillation terminates before pulmonary vein isolation
Authors: Navara Rachita , Leef George , Shenasa Fatemah , Kowalewski Christopher , Rogers Albert J. , Meckler Gabriela , Zaman Junaid A. B. , Baykaner Tina , Park Shirley , Turakhia Mintu P. , Zei Paul , Viswanathan Mohan , Wang Paul J. , Narayan Sanjiv M. 
Published: January 2018

AbstractObjectiveTo investigate mechanisms by which atrial fibrillation (AF) may terminate during ablation near the pulmonary veins before the veins are isolated (PVI).IntroductionIt remains unstudied how AF may terminate during ablation before PVs are isolated, or how patients with PV reconnection can be arrhythmia‐free. We studied patients in whom PV antral ablation terminated AF before PVI, using two independent mapping methods.MethodsWe studied patients with AF referred for ablation, in whom biatrial contact basket electrograms were studied by both an activation/phase mapping method and by a second validated mapping method reported not to create false rotational activity.ResultsIn 22 patients (age 60.1 ± 10.4, 36% persistent AF), ablation at sites near the PVs terminated AF (77% to sinus rhythm) prior to PVI. AF propagation revealed rotational (n = 20) and focal (n = 2) patterns at sites of termination by mapping method 1 and method 2. Both methods showed organized sites that were spatially concordant (P < 0.001) with similar stability (P < 0.001). Vagal slowing was not observed at sites of AF termination.DiscussionPV antral regions where ablation terminated AF before PVI exhibited rotational and focal activation by two independent mapping methods. These data provide an alternative mechanism for the success of PVI, and may explain AF termination before PVI or lack of arrhythmias despite PV reconnection. Mapping such sites may enable targeted PV lesion sets and improved freedom from AF.