bluuebunny/crossref_metadata_2025_split huggingface dataset:
Respiratory regulation by steroids in newborn rats: a sex‐specific balance between allopregnanolone and progesterone receptors
Authors: Joseph Vincent , Uppari NagaPraveena , Kouchi Hayet , De Bruyn Celia , Boukari Ryma , Bairam Aida 
Published: November 2017

New Findings
What is the central question of this study?
What are the contributions of allopregnanolone, the neuroactive metabolite of progesterone, and nuclear (nPR) and membrane (mPR) progesterone receptors to the respiratory effect of progesterone in newborn rats?

What is the main finding and its importance?
Acute progesterone injection increases the apnoea frequency, whereas finasteride (which blocks the conversion of progesterone to allopregnanolone) reduces apnoea frequency. An nPR agonist decreases apnoea frequency in males and an mPR agonist decreases apnoea frequency in males and females. Chronic injection of progesterone decreases the frequency of apnoea more efficiently in males than in females.

We tested the hypothesis that the effects of progesterone on apnoea frequency in newborn rats are the result of a balance between its neuroactive metabolite, allopregnanolone (GABAA receptor modulator), and progesterone receptors. We used male and female rats between 10 and 12 days of age and recorded respiratory and metabolic parameters (whole‐body plethysmography), and assessed the frequency and duration of apnoeas in normoxia. We tested the effects of a single injection of progesterone (4 mg kg−1, i.p.), finasteride (10 mg kg−1, i.p.; a 5α‐reductase antagonist, which blocks the conversion of progesterone to allopregnanolone), finasteride plus progesterone, or agonists of the nuclear or membrane progesterone receptors (R5020 or Org‐od‐02‐0, 4 mg kg−1). To test the hypothesis that chronic exposure to progesterone reduces the frequency of apnoeas, we used male and female rats treated daily with progesterone between postnatal days 3 and 12. The acute injection of progesterone reduced minute ventilation and metabolic rate and increased the frequency of apnoeas. Finasteride decreased the frequency of apnoeas, and finasteride plus progesterone did not increase apnoea frequency but decreased minute ventilation in female rats. Although R5020 decreased apnoea frequency only in males, Org‐od‐02‐0 decreased apnoea frequency in males and females and decreased respiratory frequency in females. Chronic progesterone treatment reduced apnoea frequency more efficiently in males than in females, but in females (not in males) an acute injection of caffeine (the gold standard for the treatment of apnoea in preterm neonates) further reduced apnoea frequency. Apnoea frequency in newborn rats is, in part, determined by a sex‐specific balance between allopregnanolone, GABAA receptors and progesterone receptors.
Complete Genome Sequences of Seven Vibrio cholerae Phages Isolated in China
Authors: Bhandare Sudhakar G. , Warry Andrew , Emes Richard D. , Su Jingliang , Barrow Paul A. , Atterbury Robert J. 
Published: November 2017

ABSTRACT

The complete genome sequences of seven closely related
Vibrio cholerae
phages isolated from environmental sites in southeastern China are reported here. Phages QH, CJY, H1, H2, H3, J2, and J3 are members of the
Podoviridae
family and are highly similar to the previously sequenced
Vibrio
phages VP2, VP5, and phiVC8.
Complete Nucleotide Sequence of Australian
Tomato spotted wilt virus
Isolate TSWV-QLD2
Authors: Moyle Richard L. , Pretorius Lara , Carvalhais Lilia C. , Schenk Peer M. 
Published: November 2017

ABSTRACT

The complete nucleotide (nt) sequence of an Australian isolate of
Tomato spotted wilt virus
was determined by deep RNA sequencing and deep small RNA sequencing. The tripartite genome consists of an 8,914-nt L segment, a 4,851-nt M segment, and a 2,987-nt S segment.
Draft Genome Sequence of the Planktic Cyanobacterium Tychonema bourrellyi, Isolated from Alpine Lentic Freshwater
Authors: Pinto Federica , Tett Adrian , Armanini Federica , Asnicar Francesco , Boscaini Adriano , Pasolli Edoardo , Zolfo Moreno , Donati Claudio , Salmaso Nico , Segata Nicola 
Published: November 2017

ABSTRACT

We describe here the draft genome sequence of the cyanobacterium
Tychonema bourrellyi
, assembled from a metagenome of a nonaxenic culture. The strain (FEM_GT703) was isolated from a freshwater sample taken from Lake Garda, Italy. The draft genome sequence represents the first assembled
T. bourrellyi
strain.
Complete Genome Sequence of Enterococcus thailandicus Strain a523 Isolated from Urban Raw Sewage
Authors: Ybazeta Gustavo , Douglas Laura , Graham Jenna , Fraleigh Nya L. , Murad Yanal , Perez Justo , Diaz-Mitoma Francisco , Tilbe Kim , Nokhbeh Reza 
Published: November 2017

ABSTRACT

We report here the first complete circularly closed genome sequence of
Enterococcus thailandicus
strain a523 isolated from raw urban sewage. This genome contains 2,646,250 bp with a G+C content of 36.8%, 2,499 genes, 2,370 protein-coding sequences, 6 rRNA operons, 65 tRNAs, and 6 clustered regularly interspaced short palindromic repeat arrays.
Draft Genome Sequence of Tepidibacter mesophilus Strain JCM 16806
T
Isolated from Soil Polluted by Crude Oil in China
Authors: Lee Chol Gyu , Yuki Masahiro , Iida Toshiya , Nakaho Kazuhiro , Ohkuma Moriya 
Published: November 2017

ABSTRACT

Here, we report the draft genome sequence of
Tepidibacter mesophilus
strain JCM 16806
T
, which was isolated from an oil field. It is composed of 3,310,272 bp and contains 3,160 protein-coding genes, 8 5S rRNAs, 3 16S rRNAs, and 69 tRNAs.
Draft Genome Sequence of
Mycobacterium
sp. Strain shizuoka-1, a Novel Mycobacterium Isolated from Groundwater of a Bathing Facility in Shizuoka, Japan
Authors: Yoshida Mitsunori , Izumiyama Shinji , Fukano Hanako , Sugiyama Kanji , Suzuki Masato , Shibayama Keigo , Hoshino Yoshihiko 
Published: November 2017

ABSTRACT

Mycobacterium
sp. strain shizuoka-1 is a rapidly growing scotochromogenic mycobacterium and was isolated from well water for a bathing facility in Shizuoka Prefecture in Japan. Here, we report the draft sequence of its genome, comprising a 6.5-Mb chromosome. This mycobacterium has 83.1% identity with
Mycobacterium rhodesiae
, a human pathogen.
Draft Genome Sequence of Sphingobium fuliginis OMI, a Bacterium That Degrades Alkylphenols and Bisphenols
Authors: Kuroda Masashi , Ogata Yuka , Yahara Tatsuya , Yokoyama Takashi , Ishizawa Hidehiro , Takada Kazuki , Inoue Daisuke , Sei Kazunari , Ike Michihiko 
Published: November 2017

ABSTRACT

Sphingobium fuliginis
OMI is a bacterium that can degrade a variety of recalcitrant alkylphenols and bisphenols. This study reports the draft genome sequence of
S. fuliginis
OMI.
Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
Authors: Paoli George C. , Wijey Chandi , Nguyen Ly-Huong , Chen Chin-Yi , Yan Xianghe , Irwin Peter L. 
Published: November 2017

ABSTRACT

Brochothrix thermosphacta
is an important meat spoilage bacterium. Here we report the genome sequences of two strains of
B. thermosphacta
isolated from ground chicken. The genome sequences were determined using long-read PacBio single-molecule real-time (SMRT) technology and are the first complete genome sequences reported for
B. thermosphacta
.
Complete Circularized Genome Sequences of Four Strains of Elizabethkingia anophelis, Including Two Novel Strains Isolated from Wild-Caught
Anopheles sinensis
Authors: Pei Dong , Nicholson Ainsley C. , Jiang Jinjin , Chen Huiying , Whitney Anne M. , Villarma Aaron , Bell Melissa , Humrighouse Ben , Rowe Lori A. , Sheth Mili , Batra Dhwani , Juieng Phalasy , Loparev Vladimir N. , McQuiston John R. , Lan Yuhao , Ma Yajun , Xu Jiannong 
Published: November 2017

ABSTRACT

We provide complete circularized genome sequences of two mosquito-derived
Elizabethkingia anophelis
strains with draft sequences currently in the public domain (R26 and Ag1), and two novel
E. anophelis
strains derived from a different mosquito species,
Anopheles sinensis
(AR4-6 and AR6-8). The genetic similarity of all four mosquito-derived strains is remarkable.
‘The more we change, the more we remain the same’: female feticide continues unabated in India
Authors: Bhattacharya Sudip , Singh Amarjeet 
Published: November 2017

In North India, preference for sons has been blamed for repeated incidents of female feticide, despite the legislation in the form of the Pre-Conception and Pre-Natal Diagnostic Techniques Act, 2002. We describe how a team of local private doctors offer package deals to pregnant women and their families in rural areas. The fetal sex is determined at night using a portable ultrasonography machine.If the fetus is a girl, an immediate induced abortion is offered in the clients’ home. If complications arise, women are advised to attend hospital. Such a patient visited us with a history of bleeding per vagina following incomplete induced abortion. This case study highlights the fact that female feticide continues to occur in India.
Categorising cancers to enable tailored care planning through a secondary analysis of cancer registration data in the UK
Authors: McConnell Hannah , White Rachel , Maher Jane 
Published: November 2017

ObjectivesThe aim of this study is to categorise cancers into broad groups based on clusters of common treatment aims, experiences and outcomes to provide a numerical framework for understanding the services required to meet the needs of people with different cancers. This framework will enable a high-level overview of care and support requirements for the whole cancer population.Setting and participantsPeople in the UK with 1 of 20 common cancers; an estimated 309 000 diagnoses in 2014, 1 679 000 people diagnosed in a 20-year period and still living in 2010 and 135 000 cancer deaths in 2014.Primary and secondary outcome measuresSurvival and stage at diagnosis data were reviewed alongside clinically led assumptions to identify commonalities and cluster cancer types into three groups. The three cancer groups were then described using incidence, prevalence and mortality data collected and reported by UK cancer registries. This was then reviewed, validated and refined following consultation.ResultsGroup 1 includes cancers with the highest survival; 5-year survival is over 80%. Group 3 cancers have shorter term survival. Five-year survival is not >20% for any cancer in this group and many do not survive over a year. Group 2 includes cancers where people typically live more than a year but are less likely to live >5 years. We estimate that the majority (64%) of people living with cancer (20 year prevalence) have a cancer type in group 1 ‘longer term survival’, but significant minorities of people have cancers in group 2 ‘intermediate survival’ (19%) and group 3 ‘shorter term survival’ (10%).ConclusionsEvery person with cancer has unique needs shaped by a multitude of factors including comorbidities, treatment regimens, patient preferences, needs, attitudes and behaviours. However, to deliver personalised care, there needs to be a high-level view of potential care requirements to support service planning.
17 Exploring ambulance conveyances to the emergency department: a descriptive analysis of non-urgent transports
Authors: Miles Jamie , O’Keeffe Colin , Jacques Richard , Stone Tony , Mason Suzanne 
Published: November 2017

ObjectivesAn NHS England report highlighted key issues in how patients were initially navigating access to healthcare. This has manifested in increased pressure on ambulance services and emergency departments (EDs) to provide high quality, safe and efficient services to manage this demand. This study aims to identify non-urgent conveyances by ambulance services to the ED that would be suitable for care at scene or an alternative response.DesignA retrospective analysis of emergency department data linked to initial pre-hospital call data (either ‘111’ or ‘999’) in 2014 in Yorkshire and Humber. A previously validated definition of non-urgent attendance at ED was adapted for pre-hospital use to identify all linked ambulance conveyances that had no ‘in-hospital’ specific investigations, treatments or follow up care during that episode. Linked data was used to identify clinical triage conditions at the time and source of call (999 or 111).SettingAll 14 acute trusts (acute hospital and ambulance service) in Yorkshire and Humber.Outcome and measuresThe number of non-urgent attendances to ED which were conveyed by ambulance was examined in terms of age, time of arrival, initial triage (AMPDS) and final ED diagnosis.Figure 1ED final diagnosis for avoidable conveyances (n=65,360)Results1,312,539 linked patient episodes were analysed which included ambulance service contact and hospital data. 4 04 348 (30.8%) of the total reported were transported by ambulance. Of all the linked conveyances, 65 360 (16.2%) were classed as non-urgent ED attendances. There were significantly increased odds of a non-urgent conveyance out of hours (OR:1.48; 95% CI:1.45 to 1.51). Of all conveyances of patients aged 16–34 (n=77,683), 24 443 (31.5%) patients were non-urgent. This compares with patients aged 75+ (n=150,668), in which 11 400 (7.1%) were considered non-urgent. 70.6% of the data was included for AMPDS analysis. This demonstrated the largest numbers of non-urgent conveyances (by code) came from falls (n=5277, 8.1%) and outside referrals such as ‘Healthcare Professionals’ (n=3983, 6.1%) and the ‘111 telephone service’ (n=9437, 14.4%). ED diagnosis analysis showed the highest proportion of patients were attending with minor injury and illness, and alcohol intoxication.Conclusions16% of ambulance conveyances to ED in 2014 were non-urgent with around 1 in 3 patients under the age of 34 conveyed with non-urgent complaints. 1 in 5 patients had a non-urgent conveyance out of hours. AMPDS analysis identified target areas for intervention including referrals from other healthcare providers. Final ED diagnosis identified specific patient target areas including minor illness and alcohol intoxication.Figure 2Figure 3Age of patients taken to ED by ambulance (avoidable)
A Novel YAP1 Inhibitor Targets CSC-Enriched Radiation-Resistant Cells and Exerts Strong Antitumor Activity in Esophageal Adenocarcinoma
Authors: Song Shumei , Xie Min , Scott Ailing W. , Jin Jiankang , Ma Lang , Dong Xiaochuan , Skinner Heath D. , Johnson Randy L. , Ding Sheng , Ajani Jaffer A. 
Published: November 2017

Abstract
Mounting evidence suggests that the Hippo coactivator Yes-associated protein 1 (YAP1) is a major mediator of cancer stem cell (CSC) properties, tumor progression, and therapy resistance as well as often a terminal node of many oncogenic pathways. Thus, targeting YAP1 may be a novel therapeutic strategy for many types of tumors with high YAP1 expression, including esophageal adenocarcinoma. However, effective YAP1 inhibitors are currently lacking. Here, we identify a small molecule (CA3) that not only has remarkable inhibitory activity on YAP1/Tead transcriptional activity but also demonstrates strong inhibitory effects on esophageal adenocarcinoma cell growth especially on YAP1 high–expressing esophageal adenocarcinoma cells both in vitro and in vivo. Remarkably, radiation-resistant cells acquire strong cancer stem cell (CSC) properties and aggressive phenotype, while CA3 can effectively suppress these phenotypes by inhibiting proliferation, inducing apoptosis, reducing tumor sphere formation, and reducing the fraction of ALDH1+ cells. Furthermore, CA3, combined with 5-FU, synergistically inhibits esophageal adenocarcinoma cell growth especially in YAP1 high esophageal adenocarcinoma cells. Taken together, these findings demonstrated that CA3 represents a new inhibitor of YAP1 and primarily targets YAP1 high and therapy-resistant esophageal adenocarcinoma cells endowed with CSC properties. Mol Cancer Ther; 17(2); 443–54. ©2017 AACR.
Occurrence of phosphatic corrosion products on bronze swords of the Warring States period buried at Lijiaba site in Chongqing, China
Authors: Fan Xiaopan , Freestone Ian C. 
Published: November 2017

Abstract
Corrosion products on three bronze swords found in tombs dating from the Warring States period at Lijiaba site, Yunyang county, Chongqing were characterized by Raman and X-ray fluorescence spectroscopies. The major corrosion products were cuprite, malachite, cerussite and cassiterite, along with the copper and lead phosphates, libethenite and pyromorphite. The presence of libethenite and pyromorphite which have been reported infrequently in bronze corrosion products were attributed to the pH, humidity and phosphorus released by the decomposition of the adjacent bodies in the burial environment.
Developing a non-invasive tool to assess the impact of oxidation on the structural integrity of historic wool in Tudor tapestries
Authors: Kissi Nanette , Curran Katherine , Vlachou-Mogire Constantina , Fearn Tom , McCullough Lisa 
Published: November 2017

Abstract
Near infrared spectroscopy (NIR) can be a useful diagnostic tool in the assessment of quality in textile, agricultural and various other industries. In cultural heritage applications, NIR as a non-invasive assessment tool is advantageous for understanding the composition or degradation of fragile historic objects that cannot be sampled. The direct interpretation of measured NIR spectra is complicated by overlapping bands produced from overtone and combination bands of chemical bonds. However, in combination with multivariate partial least squares (PLS) models and principal component analysis, the physicochemical properties of historic materials can potentially be identified by calibrating against a measured component. In this research, wool fibres from a sacrificial collection of historic tapestry fragments housed at Hampton Court Palace were analysed. The cystine oxidation products of historic wools which can be related to the embrittlement of fibres and ultimately fibre loss, were recorded using Fourier transform infrared (FTIR) spectroscopy. A PLS predictive model was then developed using previously measured NIR spectra for the same historic tapestry fragments to predict the ratios of the cystine oxidation species in historic wools measured in this research. The prediction of a validation set of historic tapestry fragments with known oxidation product ratios was found to be accurate to root mean squared errors of prediction (RMSEP) of 0.11 for cysteic acid/cystine dioxide, 0.03 for an oxidation ratio of cysteic acid/cystine monoxide and 0.06 for cystine dioxide/cystine monoxide. The model was applied successfully to an independent set of historic tapestry fragments with unknown oxidation ratios with an M-distance pass limit of 3. The models were also applied to NIR measurements of historic tapestries on open display with limited success. Alternative methods to investigate and account for errors in this research are proposed. The potential for NIR to be used by conservators as a non-invasive tool for the assessment of textiles, complementing current condition audit practices in use at heritage institutions is also discussed.
Nerve transfer to biceps to restore elbow flexion and supination in children with obstetrical brachial plexus palsy
Authors: Murison J. , Jehanno P. , Fitoussi F. 
Published: November 2017

Purpose Nerve transfers to restore elbow flexion have been described for traumatic brachial plexus palsy in adults. Indications are less frequent in infants and the results are less published. Methods Ten patients with obstetrical brachial plexus palsy were operated on for lack of flexion against gravity with ulnar or median nerve transfer to biceps motor branch. The primary endpoint was improvement in elbow flexion and supination. Results Mean age at surgery was 12.5 months and mean follow-up was 2.6 years. The Active Movement Scale (AMS) was used to evaluate elbow flexion and forearm supination. At the last follow-up, the average AMS score improved from 0.3 to 5.7 for elbow flexion and from 0.6 to 5.8 for forearm supination. There was no statistical correlation between the age at surgery and the AMS score 18 months post-operatively. Conclusions Nerve transfer to the biceps motor branch can improve elbow flexion and forearm supination in selected patients with upper lesions and can be safely performed until the age of two years.
Indications for hydrodilatation for frozen shoulder
Authors: Rymaruk S. , Peach C. 
Published: November 2017

Frozen shoulder causes significant functional disability and pain in a population group constituted by patients who are often middle-aged and working. Frozen shoulder remains poorly understood. The available literature is limited and often prone to bias. A rapid, non-surgical and cost-effective treatment that reduces pain and restores function is an attractive option. Hydrodilatation is a potential first-line treatment of frozen shoulder in secondary care. Cite this article: EFORT Open Rev 2017;2:462–468. DOI: 10.1302/2058-5241.2.160061
New Ecuadorian cinema: Small, glocal and plurinational
Authors: Coryat Diana , Zweig Noah 
Published: November 2017

Abstract
This article conceptualizes New Ecuadorian cinema as small, glocal and plurinational. It makes the case that it is a propitious time to study this emergent cinema. However, while it is a heterogeneous film scene, this article argues that it is still traversed by long-standing ethno-racial, class and regional inequalities. After inserting Ecuador’s largely unknown film history into regional trends, different spheres of production are analysed, including mestizo fictionalized narratives, activist documentaries, indigenous and community filmmaking and coastal ‘guerilla’ filmmaking. The article looks at both the accomplishments of the sector and current challenges. It proposes that more state and private funds invest in fostering plurinational and regional initiatives, and cultivating diverse makers and audiences.
A mixed methods study of “English Corners” funded by the Taiwan Ministry of Education “Program for Promoting Teaching Excellence of Universities”
Authors: Reynolds Barry Lee 
Published: November 2017

Abstract
This investigation explored Taiwanese university students’ and professors’ perceptions of the effectiveness of “English Corner” in improving second language ability. Qualitative and quantitative data was collected through student attendee questionnaires (n=256); teaching logs (n=2); and teaching excellence university professor questionnaires (n=70). Item analysis and factor analysis was conducted to uncover factors affecting students’ perceptions of English Corner and qualitative content analysis using In Vivo and Emotion coding revealed themes affecting “English Corner” success or failure. Overall, findings indicate “English Corner” has the most influence on students’ willingness to speak in English and their enjoyment in speaking English. These results are encouraged by administration supported flexible teachers that apply various scaffolding techniques during lessons. However, analysis also indicated that success of “English Corner” is highly contextualized, with professors from teaching excellence universities possessing a dichotomized view of “English Corner” effectiveness.