bluuebunny/crossref_metadata_2025_split huggingface dataset:
Injury-activated glial cells promote wound healing of the adult skin in mice
Authors: Parfejevs Vadims , Debbache Julien , Shakhova Olga , Schaefer Simon M. , Glausch Mareen , Wegner Michael , Suter Ueli , Riekstina Una , Werner Sabine , Sommer Lukas 
Published: January 2018

AbstractCutaneous wound healing is a complex process that aims to re-establish the original structure of the skin and its functions. Among other disorders, peripheral neuropathies are known to severely impair wound healing capabilities of the skin, revealing the importance of skin innervation for proper repair. Here, we report that peripheral glia are crucially involved in this process. Using a mouse model of wound healing, combined with in vivo fate mapping, we show that injury activates peripheral glia by promoting de-differentiation, cell-cycle re-entry and dissemination of the cells into the wound bed. Moreover, injury-activated glia upregulate the expression of many secreted factors previously associated with wound healing and promote myofibroblast differentiation by paracrine modulation of TGF-β signalling. Accordingly, depletion of these cells impairs epithelial proliferation and wound closure through contraction, while their expansion promotes myofibroblast formation. Thus, injury-activated glia and/or their secretome might have therapeutic potential in human wound healing disorders.
Author Correction: Hot carrier cooling mechanisms in halide perovskites
Authors: Fu Jianhui , Xu Qiang , Han Guifang , Wu Bo , Huan Cheng Hon Alfred , Leek Meng Lee , Sum Tze Chien 
Published: January 2018

The original version of this Article omitted an affiliation of Cheng Hon Alfred Huan: ‘Institute of Materials Research and Engineering (IMRE), A*STAR, 2 Fusionopolis Way, Innovis #08-03 138634, Singapore’.This has now been corrected in both the PDF and HTML versions of the article.
Hydrogen blistering under extreme radiation conditions
Authors: Sznajder Maciej , Geppert Ulrich , Dudek Mirosław R. 
Published: January 2018

AbstractMetallic surfaces, exposed to a proton flux, start to degradate by molecular hydrogen blisters. These are created by recombination of protons with metal electrons. Continued irradiation progresses blistering, which is undesired for many technical applications. In this work, the effect of the proton flux magnitude onto the degradation of native metal oxide layers and its consequences for blister formation has been examined. To study this phenomenon, we performed proton irradiation experiments of aluminium surfaces. The proton kinetic energy was chosen so that all recombined hydrogen is trapped within the metal structure. As a result, we discovered that intense proton irradiation increases the permeability of aluminium oxide layers for hydrogen atoms, thereby counteracting blister formation. These findings may improve the understanding of the hydrogen blistering process, are valid for all metals kept under terrestrial ambient conditions, and important for the design of proton irradiation tests.
Determinants of postnatal spleen tissue regeneration and organogenesis
Authors: Tan Jonathan K. H. , Watanabe Takeshi 
Published: January 2018

AbstractThe spleen is an organ that filters the blood and is responsible for generating blood-borne immune responses. It is also an organ with a remarkable capacity to regenerate. Techniques for splenic auto-transplantation have emerged to take advantage of this characteristic and rebuild spleen tissue in individuals undergoing splenectomy. While this procedure has been performed for decades, the underlying mechanisms controlling spleen regeneration have remained elusive. Insights into secondary lymphoid organogenesis and the roles of stromal organiser cells and lymphotoxin signalling in lymph node development have helped reveal similar requirements for spleen regeneration. These factors are now considered in the regulation of embryonic and postnatal spleen formation, and in the establishment of mature white pulp and marginal zone compartments which are essential for spleen-mediated immunity. A greater understanding of the cellular and molecular mechanisms which control spleen development will assist in the design of more precise and efficient tissue grafting methods for spleen regeneration on demand. Regeneration of organs which harbour functional white pulp tissue will also offer novel opportunities for effective immunotherapy against cancer as well as infectious diseases.
Understanding turbulent free-surface vortex flows using a Taylor-Couette flow analogy
Authors: Mulligan Sean , De Cesare Giovanni , Casserly John , Sherlock Richard 
Published: January 2018

AbstractFree-surface vortices have long been studied to develop an understanding of similar rotating flow phenomena observed in nature and technology. However, a complete description of its turbulent three-dimensional flow field still remains elusive. In contrast, the related Taylor-Couette flow system has been well explicated which classically exhibits successive instability phases manifested in so-called Taylor vortices. In this study, observations made on the turbulent free-surface vortex revealed distinguishable, time-dependent “Taylor-like” vortices in the secondary flow field similar to the Taylor-Couette flow system. The observations were enabled by an original application of 2D ultrasonic Doppler velocity profiling complemented with laser induced fluorescence dye observations. Additional confirmation was provided by three-dimensional numerical simulations. Using Rayleigh’s stability criterion, we analytically show that a wall bounded free-surface vortex can indeed become unstable due to a centrifugal driving force in a similar manner to the Taylor-Couette flow. Consequently, it is proposed that the free-surface vortex can be treated analogously to the Taylor-Couette flow permitting advanced conclusions to be drawn on its flow structure and the various states of free-surface vortex flow stability.
Newborn screening of glucose-6-phosphate dehydrogenase deficiency in Guangxi, China: determination of optimal cutoff value to identify heterozygous female neonates
Authors: Fu Chunyun , Luo Shiyu , Li Qifei , Xie Bobo , Yang Qi , Geng Guoxing , Lin Caijuan , Su Jiasun , Zhang Yue , Wang Jin , Qin Zailong , Luo Jingsi , Chen Shaoke , Fan Xin 
Published: January 2018

AbstractThe aim of this study is to assess the disease incidence and mutation spectrum of glucose-6-phosphate dehydrogenase (G6PD) deficiency in Guangxi, China, and to determine an optimal cutoff value to identify heterozygous female neonates. A total of 130, 635 neonates were screened from the year of 2013 to 2017. Neonates suspected for G6PD deficiency were further analyzed by quantitatively enzymatic assay and G6PD mutation analysis. The overall incidence of G6PD deficiency was 7.28%. A total of 14 G6PD mutations were identified, and different mutations lead to varying levels of G6PD enzymatic activities. The best cut-off value of G6PD activity in male subjects is 2.2 U/g Hb, same as conventional setting. In female population, however, the cut-off value is found to be 2.8 U/g Hb (sensitivity: 97.5%, specificity: 87.7%, AUC: 0.964) to best discriminate between normal and heterozygotes, and 1.6 U/g Hb (sensitivity: 82.2%, specificity: 85.9%, AUC: 0.871) between heterozygotes and deficient subjects. In conclusion, we have conducted a comprehensive newborn screening of G6PD deficiency in a large cohort of population from Guangxi, China, and first established a reliable cut-off value of G6PD activity to distinguish heterozygous females from either normal or deficient subjects.
Detection of American Football Head Impacts Using Biomechanical Features and Support Vector Machine Classification
Authors: Wu Lyndia C. , Kuo Calvin , Loza Jesus , Kurt Mehmet , Laksari Kaveh , Yanez Livia Z. , Senif Daniel , Anderson Scott C. , Miller Logan E. , Urban Jillian E. , Stitzel Joel D. , Camarillo David B. 
Published: January 2018

AbstractAccumulation of head impacts may contribute to acute and long-term brain trauma. Wearable sensors can measure impact exposure, yet current sensors do not have validated impact detection methods for accurate exposure monitoring. Here we demonstrate a head impact detection method that can be implemented on a wearable sensor for detecting field football head impacts. Our method incorporates a support vector machine classifier that uses biomechanical features from the time domain and frequency domain, as well as model predictions of head-neck motions. The classifier was trained and validated using instrumented mouthguard data from collegiate football games and practices, with ground truth data labels established from video review. We found that low frequency power spectral density and wavelet transform features (10~30 Hz) were the best performing features. From forward feature selection, fewer than ten features optimized classifier performance, achieving 87.2% sensitivity and 93.2% precision in cross-validation on the collegiate dataset (n = 387), and over 90% sensitivity and precision on an independent youth dataset (n = 32). Accurate head impact detection is essential for studying and monitoring head impact exposure on the field, and the approach in the current paper may help to improve impact detection performance on wearable sensors.
HPV-16 virions can remain infectious for 2 weeks on senescent cells but require cell cycle re-activation to allow virus entry
Authors: Broniarczyk Justyna , Ring Nadja , Massimi Paola , Giacca Mauro , Banks Lawrence 
Published: January 2018

AbstractSuccessful infection with Human Papillomaviruses requires mitosis, when incoming viral genomes gain access to nuclear components. However, very little is known about how long HPV particles can remain infectious in non-dividing cells or in which cellular compartments these viruses may reside. To investigate these questions we have used BJ cells as a reversible model of senescence and show that HPV-16 can only infect early-passage proliferating cells. Late-passage senescent cells are resistant to HPV infection, but this can be reversed by inducing cell cycle re-entry with a p53 siRNA. In senescent cells we find that efficient virus entry can be attained upon cell cycle re-entry 16 days after infection, demonstrating that HPV can persist for 2 weeks prior to induction of mitosis. However, exposing cells to anti-HPV-16 L1 neutralising antibody blocks infection at these late time points, suggesting that the virions reside near the cell surface. Indeed, immunofluorescence analysis shows that virions accumulate on the cell surface of senescent cells and only enter endocytic vesicles upon stimulation with p53 siRNA. These results demonstrate that HPV-16 virions can remain viable on a non-dividing cell for extended periods of time, but are nonetheless vulnerable to antibody-induced neutralisation throughout.
Ancient Human Migrations to and through Jammu Kashmir- India were not of Males Exclusively
Authors: Sharma Indu , Sharma Varun , Khan Akbar , Kumar Parvinder , Rai Ekta , Bamezai Rameshwar N. K. , Vilar Miguel , Sharma Swarkar 
Published: January 2018

AbstractJammu and Kashmir (J&K), the Northern most State of India, has been under-represented or altogether absent in most of the phylogenetic studies carried out in literature, despite its strategic location in the Himalayan region. Nonetheless, this region may have acted as a corridor to various migrations to and from mainland India, Eurasia or northeast Asia. The belief goes that most of the migrations post-late-Pleistocene were mainly male dominated, primarily associated with population invasions, where female migration may thus have been limited. To evaluate female-centered migration patterns in the region, we sequenced 83 complete mitochondrial genomes of unrelated individuals belonging to different ethnic groups from the state. We observed a high diversity in the studied maternal lineages, identifying 19 new maternal sub-haplogroups (HGs). High maternal diversity and our phylogenetic analyses suggest that the migrations post-Pleistocene were not strictly paternal, as described in the literature. These preliminary observations highlight the need to carry out an extensive study of the endogamous populations of the region to unravel many facts and find links in the peopling of India.
An orthotopic mouse model of gastric cancer invasion and metastasis
Authors: Busuttil Rita A. , Liu David S. , Di Costanzo Natasha , Schröder Jan , Mitchell Catherine , Boussioutas Alex 
Published: January 2018

AbstractGastric cancer is a leading cause of cancer death worldwide, with advanced stage being correlated to the level of tumour invasion and metastasis. Current research is heavily focused on the identification and development of efficacious therapeutics targeting these fundamental hallmarks of cancer, however there are currently no animal models that mimic the invasive phenotypes observed in humans. To address this we have developed an orthotopic mouse model whereby gastric cancer cell lines are tagged with luciferase and injected into the subserosal layer of the stomach. This allows for the monitoring of primary tumour growth and metastasis in real-time as well as quantitation of the degree of tumour invasion through the stomach wall by immunohistochemistry. We have three models based on the degree of invasion and metastasis that are cell line specific: The AGS cells develop into invasive tumours by 4-weeks with no evidence of metastases, MKN45 cells are moderately metastatic with minimal invasion till week 2 and MKN28 cells are highly metastatic and fully invasive by week 1. These models have utility as a tool for testing the efficacy of anti-tumour, anti-invasive and anti-metastatic therapies in the setting of gastric cancer, which currently has poor treatment options.
Four-dimensional entanglement distribution over 100 km
Authors: Ikuta Takuya , Takesue Hiroki 
Published: January 2018

AbstractHigh-dimensional quantum entanglement can enrich the functionality of quantum information processing. For example, it can enhance the channel capacity for linear optic superdense coding and decrease the error rate threshold of quantum key distribution. Long-distance distribution of a high-dimensional entanglement is essential for such advanced quantum communications over a communications network. Here, we show a long-distance distribution of a four-dimensional entanglement. We employ time-bin entanglement, which is suitable for a fibre transmission, and implement scalable measurements for the high-dimensional entanglement using cascaded Mach-Zehnder interferometers. We observe that a pair of time-bin entangled photons has more than 1 bit of secure information capacity over 100 km. Our work constitutes an important step towards secure and dense quantum communications in a large Hilbert space.
Carbohydrates protect protein against abiotic fragmentation by soil minerals
Authors: Reardon Patrick N. , Walter Eric D. , Marean-Reardon Carrie L. , Lawrence Chad W. , Kleber Markus , Washton Nancy M. 
Published: January 2018

AbstractThe degradation and turnover of soil organic matter is an important part of global carbon cycling and of particular importance with respect to attempts to predict the response of ecosystems to global climate change. Thus, it is important to mechanistically understand the processes by which organic matter can be degraded in the soil environment, including contact with reactive or catalytic mineral surfaces. We have characterized the outcome of the interaction of two minerals, birnessite and kaolinite, with two disaccharides, cellobiose and trehalose. These results show that birnessite reacts with and degrades the carbohydrates, while kaolinite does not. The reaction of disaccharides with birnessite produces Mn(II), indicating that degradation of the disaccharides is the result of their oxidation by birnessite. Furthermore, we find that both sugars can inhibit the degradation of a model protein by birnessite, demonstrating that the presence of one organic constituent can impact abiotic degradation of another. Therefore, both the reactivity of the mineral matrix and the presence of certain organic constituents influence the outcomes of abiotic degradation. These results suggest the possibility that microorganisms may be able to control the functionality of exoenzymes through the concomitant excretion of protective organic substances, such as those found in biofilms.
Postural adaptations to unilateral knee joint hypomobility induced by orthosis wear during gait initiation
Authors: Delafontaine A. , Fourcade P. , Honeine J. L. , Ditcharles S. , Yiou E. 
Published: January 2018

AbstractBalance control and whole-body progression during gait initiation (GI) involve knee-joint mobility. Single knee-joint hypomobility often occurs with aging, orthopedics or neurological conditions. The goal of the present study was to investigate the capacity of the CNS to adapt GI organization to single knee-joint hypomobility induced by the wear of an orthosis. Twenty-seven healthy adults performed a GI series on a force-plate in the following conditions: without orthosis (“control”), with knee orthosis over the swing leg (“orth-swing”) and with the orthosis over the contralateral stance leg (“orth-stance”). In orth-swing, amplitude of mediolateral anticipatory postural adjustments (APAs) and step width were larger, execution phase duration longer, and anteroposterior APAs smaller than in control. In orth-stance, mediolateral APAs duration was longer, step width larger, and amplitude of anteroposterior APAs smaller than in control. Consequently, step length and progression velocity (which relate to the “motor performance”) were reduced whereas stability was enhanced compared to control. Vertical force impact at foot-contact did not change across conditions, despite a smaller step length in orthosis conditions compared to control. These results show that the application of a local mechanical constraint induced profound changes in the global GI organization, altering motor performance but ensuring greater stability.
Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome
Authors: Yabe Ichiro , Yaguchi Hiroaki , Kato Yasutaka , Miki Yasuo , Takahashi Hidehisa , Tanikawa Satoshi , Shirai Shinichi , Takahashi Ikuko , Kimura Mari , Hama Yuka , Matsushima Masaaki , Fujioka Shinsuke , Kano Takahiro , Watanabe Masashi , Nakagawa Shin , Kunieda Yasuyuki , Ikeda Yoshio , Hasegawa Masato , Nishihara Hiroshi , Ohtsuka Toshihisa , Tanaka Shinya , Tsuboi Yoshio , Hatakeyama Shigetsugu , Wakabayashi Koichi , Sasaki Hidenao 
Published: January 2018

AbstractClinical diagnosis of progressive supranuclear palsy (PSP) is sometimes difficult because various phenotypes have been identified. Here, we report a mutation in the bassoon (BSN) gene in a family with PSP-like syndrome. Their clinical features resembled not only those of PSP patients but also those of individuals with multiple system atrophy and Alzheimer’s disease. The neuropathological findings showed a novel three + four repeat tauopathy with pallido-luysio-nigral degeneration and hippocampal sclerosis. Whole-exome analysis of this family identified a novel missense mutation in BSN. Within the pedigree, the detected BSN mutation was found only in affected individuals. Further genetic analyses were conducted in probands from four other pedigrees with PSP-like syndrome and in 41 sporadic cases. Three missense mutations in BSN that are very rarely listed in databases of healthy subjects were found in four sporadic cases. Western blot analysis of tau following the overexpression of wild-type or mutated BSN revealed the possibility that wild-type BSN reduced tau accumulation, while mutated BSN lost this function. An association between BSN and neurological diseases has not been previously reported. Our results revealed that the neurodegenerative disorder associated with the original proband’s pedigree is a novel tauopathy, differing from known dementia and parkinsonism syndromes, including PSP.
The GluN2A Subunit Regulates Neuronal NMDA receptor-Induced Microglia-Neuron Physical Interactions
Authors: Eyo Ukpong B. , Bispo Ashley , Liu Junting , Sabu Sruchika , Wu Rong , DiBona Victoria L. , Zheng Jiaying , Murugan Madhuvika , Zhang Huaye , Tang Yamei , Wu Long-Jun 
Published: January 2018

AbstractMicroglia are known to engage in physical interactions with neurons. However, our understanding of the detailed mechanistic regulation of microglia-neuron interactions is incomplete. Here, using high resolution two photon imaging, we investigated the regulation of NMDA receptor-induced microglia-neuron physical interactions. We found that the GluN2A inhibitor NVPAAM007, but not the GluN2B inhibitor ifenprodil, blocked the occurrence of these interactions. Consistent with the well-known developmental regulation of the GluN2A subunit, these interactions are absent in neonatal tissues. Furthermore, consistent with a preferential synaptic localization of GluN2A subunits, there is a differential sensitivity of their occurrence between denser (stratum radiatum) and less dense (stratum pyramidale) synaptic sub-regions of the CA1. Finally, consistent with differentially expressed GluN2A subunits in the CA1 and DG areas of the hippocampus, these interactions could not be elicited in the DG despite robust microglial chemotactic capabilities. Together, these results enhance our understanding of the mechanistic regulation of NMDA receptor-dependent microglia-neuronal physical interactions phenomena by the GluN2A subunit that may be relevant in the mammalian brain during heightened glutamatergic neurotransmission such as epilepsy and ischemic stroke.
Supra-pharmacological concentration of capsaicin stimulates brown adipogenesis through induction of endoplasmic reticulum stress
Authors: Kida Ryosuke , Noguchi Taiki , Murakami Masaru , Hashimoto Osamu , Kawada Teruo , Matsui Tohru , Funaba Masayuki 
Published: January 2018

AbstractWe previously showed that brown (pre)adipocytes express Trpv1, a capsaicin receptor, and that capsaicin stimulates differentiation of brown preadipocytes in the late stages of brown adipogenesis. The present study revealed that treatment with 100 μM capsaicin stimulates brown adipogenesis by inducing endoplasmic reticulum (ER) stress. Treatment with capsaicin (100 μM) during brown adipogenesis enhanced lipid accumulation and the expression of Ucp1, a gene selectively expressed in brown adipocytes. Capsaicin treatment also caused an increase in the cytosolic calcium concentration even when extracellular calcium was removed. I-RTX, a Trpv1 inhibitor, did not modulate the increase in cytosolic calcium concentration, lipid accumulation or Ucp1 expression. Previous studies revealed that the release of calcium from the ER induces ER stress, leading to the conversion of X-box binding protein 1 (Xbp1) pre-mRNA to spliced Xbp1 (sXbp1) as well as the up-regulation of Chop expression. Capsaicin treatment increased the expression of sXbp1 and Chop in brown preadipocytes and did not enhance lipid accumulation or Ucp1 expression in Xbp1 knockdown cells. The present results describe a novel mechanism of brown adipogenesis regulation via ER stress that is induced by a supra-pharmacological concentration of capsaicin.