bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ozone Improves the Aromatic Fingerprint of White Grapes
Authors: Río Segade Susana , Vilanova Mar , Giacosa Simone , Perrone Irene , Chitarra Walter , Pollon Matteo , Torchio Fabrizio , Boccacci Paolo , Gambino Giorgio , Gerbi Vincenzo , Rolle Luca 
Published: November 2017

AbstractOzone, a powerful oxidative stressor, has been recently used in wine industry as sanitizing agent to reduce spoilage microflora on grapes. In this study, we evaluated ozone-induced metabolic and molecular responses during postharvest grape dehydration. Ozone increased the contents of total volatile organic compounds (VOCs), which have a great impact on the organoleptic properties of grapes and wines. Among terpenes, responsible for floral and fruity aroma, linalool, geraniol and nerol were the major aromatic markers of Moscato bianco grapes. They were significantly affected by the long-term ozone treatment, increasing their concentration in the last phases of dehydration (>20% weight loss). At molecular level, our results demonstrated that both postharvest dehydration and ozone exposure induce the biosynthesis of monoterpenes via methylerythritol phosphate (MEP) pathway and of aldehydes from lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway. Therefore, transcriptional changes occurred and promoted the over-production of many important volatile compounds for the quality of white grapes.
Gas hydrate saturations estimated from pore-and fracture-filling gas hydrate reservoirs in the Qilian Mountain permafrost, China
Authors: Xiao Kun , Zou Changchun , Lu Zhenquan , Deng Juzhi 
Published: November 2017

AbstractAccurate calculation of gas hydrate saturation is an important aspect of gas hydrate resource evaluation. The effective medium theory (EMT model), the velocity model based on two-phase medium theory (TPT model), and the two component laminated media model (TCLM model), are adopted to investigate the characteristics of acoustic velocity and gas hydrate saturation of pore- and fracture-filling reservoirs in the Qilian Mountain permafrost, China. The compressional wave (P-wave) velocity simulated by the EMT model is more consistent with actual log data than the TPT model in the pore-filling reservoir. The range of the gas hydrate saturation of the typical pore-filling reservoir in hole DKXX-13 is 13.0~85.0%, and the average value of the gas hydrate saturation is 61.9%, which is in accordance with the results by the standard Archie equation and actual core test. The P-wave phase velocity simulated by the TCLM model can be transformed directly into the P-wave transverse velocity in a fracture-filling reservoir. The range of the gas hydrate saturation of the typical fracture-filling reservoir in hole DKXX-19 is 14.1~89.9%, and the average value of the gas hydrate saturation is 69.4%, which is in accordance with actual core test results.
Preparation of ZnSO4·7H2O using filter cake enriched in calcium and magnesium from the process of zinc hydrometallurgy
Authors: Li Bo , Wang Xuanbing , Wei Yonggang , Wang Hua , Hu Guanglai 
Published: November 2017

AbstractZnSO4∙7H2O was prepared using a filter cake enriched in calcium and magnesium that was generated during the process of zinc hydrometallurgy. The study was optimized to obtain process parameters. The results show that the optimal acid leaching parameters are a solid-to-liquid ratio of 1:3.5, a sulfuric acid concentration of 16%, an acid leaching time of 20 min, a final pH of 4.1–4.4, a cooling and settling time of 120 min, an oxidation time of 20 min, a stirring speed of 300 r/min, a H2O2 dosage of 25 mL/L, a crystallization temperature of 20 °C, and a crystallization time of 60 min. The ZnSO4∙7H2O content in the product is 98.6%, and the zinc recovery efficiency is 97.5%. This process is characterized by simple flow and low cost, while the circulation and accumulation problems with calcium and magnesium ions in the zinc hydrometallurgy process are also solved.
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Yu Xiao-Wei , Sun Wei-Hong , Wang Ying-Zheng , Xu Yan 
Published: November 2017

AbstractThe methylotrophic yeast Komagataella phaffii (Pichia pastoris) has been developed into a highly successful system for heterologous protein expression in both academia and industry. However, overexpression of recombinant protein often leads to severe burden on the physiology of K. phaffii and triggers cellular stress. To elucidate the global effect of protein overexpression, we set out to analyze the differential transcriptome of recombinant strains with 12 copies and a single copy of phospholipase A2 gene (PLA2) from Streptomyces violaceoruber. Through GO, KEGG and heat map analysis of significantly differentially expressed genes, the results indicated that the 12-copy strain suffered heavy cellular stress. The genes involved in protein processing and stress response were significantly upregulated due to the burden of protein folding and secretion, while the genes in ribosome and DNA replication were significantly downregulated possibly contributing to the reduced cell growth rate under protein overexpression stress. Three most upregulated heat shock response genes (CPR6, FES1, and STI1) were co-overexpressed in K. phaffii and proved their positive effect on the secretion of reporter enzymes (PLA2 and prolyl endopeptidase) by increasing the production up to 1.41-fold, providing novel helper factors for rational engineering of K. phaffii.
A pH-induced conformational switch in a tyrosine kinase inhibitor identified by electronic spectroscopy and quantum chemical calculations
Authors: Khattab Muhammad , Wang Feng , Clayton Andrew H. A. 
Published: November 2017

AbstractTyrosine kinase inhibitors (TKIs) are a major class of drug utilised in the clinic. During transit to their cognate kinases, TKIs will encounter different pH environments that could have a major influence on TKI structure. To address this, we report UV-Vis spectroscopic and computational studies of the TKI, AG1478, as a function of pH. The electronic absorption spectrum of AG1478 shifted by 10 nm (from 342 nm to 332 nm) from acid to neutral pH and split into two peaks (at 334 nm and 345 nm) in highly alkaline conditions. From these transitions, the pKa value was calculated as 5.58 ± 0.01. To compute structures and spectra, time-dependent density functional theory (TD-DFT) calculations were performed along with conductor-like polarizable continuum model (CPCM) to account for implicit solvent effect. On the basis of the theoretical spectra, we could assign the AG1478 experimental spectrum at acidic pH to a mixture of two twisted conformers (71% AG1478 protonated at quinazolyl nitrogen N(1) and 29% AG1478 protonated at quinazolyl nitrogen N(3)) and at neutral pH to the neutral planar conformer. The AG1478 absorption spectrum (pH 13.3) was fitted to a mixture of neutral (70%) and NH-deprotonated species (30%). These studies reveal a pH-induced conformational transition in a TKI.
Identification of DmTTLL5 as a Major Tubulin Glutamylase in the Drosophila Nervous System
Authors: Devambez Isabelle , van Dijk Juliette , Benlefki Salim , Layalle Sophie , Grau Yves , Rogowski Krzysztof , Parmentier Marie-Laure , Soustelle Laurent 
Published: November 2017

AbstractMicrotubules (MTs) play crucial roles during neuronal life. They are formed by heterodimers of alpha and beta-tubulins, which are subjected to several post-translational modifications (PTMs). Amongst them, glutamylation consists in the reversible addition of a variable number of glutamate residues to the C-terminal tails of tubulins. Glutamylation is the most abundant MT PTM in the mammalian adult brain, suggesting that it plays an important role in the nervous system (NS). Here, we show that the previously uncharacterized CG31108 gene encodes an alpha-tubulin glutamylase acting in the Drosophila NS. We show that this glutamylase, which we named DmTTLL5, initiates MT glutamylation specifically on alpha-tubulin, which are the only glutamylated tubulin in the Drosophila brain. In DmTTLL5 mutants, MT glutamylation was not detected in the NS, allowing for determining its potential function. DmTTLL5 mutants are viable and we did not find any defect in vesicular axonal transport, synapse morphology and larval locomotion. Moreover, DmTTLL5 mutant flies display normal negative geotaxis behavior and their lifespan is not altered. Thus, our work identifies DmTTLL5 as the major enzyme responsible for initiating neuronal MT glutamylation specifically on alpha-tubulin and we show that the absence of MT glutamylation is not detrimental for Drosophila NS function.
Habitat foraging niche of a High Arctic zooplanktivorous seabird in a changing environment
Authors: Jakubas Dariusz , Wojczulanis-Jakubas Katarzyna , Iliszko Lech M. , Strøm Hallvard , Stempniewicz Lech 
Published: November 2017

AbstractHere, we model current and future distribution of a foraging Arctic endemic species, the little auk (Alle alle), a small zooplanktivorous Arctic seabird. We characterized environmental conditions [sea depth, sea surface temperature (SST), marginal sea ice zone (MIZ)] at foraging positions of GPS-tracked individuals from three breeding colonies in Svalbard: one located at the southern rim of the Arctic zone (hereafter ‘boreo-Arctic’) and two in the high-Arctic zone on Spitsbergen (‘high-Arctic’). The birds from one ‘high-Arctic’ colony, influenced by cold Arctic water, foraged in the shallow shelf zone near the colony. The birds from remaining colonies foraged in a wider range of depths, in a higher SST zone (‘boreo-Arctic’) or in the productive but distant MIZ (second ‘high-Arctic’ colony). Given this flexible foraging behaviour, little auks may be temporarily resilient to moderate climate changes. However, our fuzzy logic models of future distribution under scenarios of 1 °C and 2 °C SST increase predict losses of suitable foraging habitat for the majority of little auk colonies studied. Over longer time scales negative consequences of global warming are inevitable. The actual response of little auks to future environmental conditions will depend on the range of their plasticity and pace of ecosystem changes.
A Distinct Endocytic Mechanism of Functionalized-Silica Nanoparticles in Breast Cancer Stem Cells
Authors: Sun Jiadong , Liu Yajing , Ge Min , Zhou Guoqiang , Sun Wentong , Liu Dandan , Liang Xing-Jie , Zhang Jinchao 
Published: November 2017

AbstractNanoparticles provide new fields for life medical science application, including targeted-drug delivery and cancer treatment. To maximize the delivery efficiency of nanoparticle, one must understand the uptake mechanism of nanoparticle in cells, which may determine their ultimate fate and localization in cells. Recently, the proposed-cancer stem cell (CSC) theory has been attracted great attention and regarded as new targets for the new nanodrug developmet and cancer therapies. The interaction between nanoparticles and cancer cells has been extensively studied, but the uptake mechanism of nanoparticles in CSCs has received little attention. Here, we use the pharmacological inhibitors of major endocytic pathways to study the silica nanoparticle (SiNP) uptake mechanisms in the human breast adenocarcinoma cell line (MCF-7) and MCF-7-derived breast cancer stem cells (BCSCs). The results demonstrate that the uptake of SiNPs, particularly amino-functionalized SiNPs, in MCF-7 cells is strongly affected by the actin depolymerization, whereas BCSCs more strongly inhibit the amino-functionalized SiNP uptake after the scavenger receptor disruption. These findings indicate a distinct endocytic mechanism of functionalized SiNPs in BCSCs, which is significant for designing ideal nanosized drug delivery systems and improving the selectivity for CSC-targeted therapy.
Occurrence and persistence of magnetic elements in the quiet Sun
Authors: Giannattasio F. , Berrilli F. , Consolini G. , Del Moro D. , Gošić M. , Bellot Rubio L. 
Published: November 2017

Context.Turbulent convection efficiently transports energy up to the solar photosphere, but its multi-scale nature and dynamic properties are still not fully understood. Several works in the literature have investigated the emergence of patterns of convective and magnetic nature in the quiet Sun at spatial and temporal scales from granular to global.Aims.To shed light on the scales of organisation at which turbulent convection operates, and its relationship with the magnetic flux therein, we studied characteristic spatial and temporal scales of magnetic features in the quiet Sun.Methods.Thanks to an unprecedented data set entirely enclosing a supergranule, occurrence and persistence analysis of magnetogram time series were used to detect spatial and long-lived temporal correlations in the quiet Sun and to investigate their nature.Results.A relation between occurrence and persistence representative for the quiet Sun was found. In particular, highly recurrent and persistent patterns were detected especially in the boundary of the supergranular cell. These are due to moving magnetic elements undergoing motion that behaves like a random walk together with longer decorrelations (~2 h) with respect to regions inside the supergranule. In the vertices of the supegranular cell the maximum observed occurrence is not associated with the maximum persistence, suggesting that there are different dynamic regimes affecting the magnetic elements.
Schmerz in der Gruppe lösen
Authors: Kürz-Weis Beate 
Published: November 2017

ZusammenfassungWieder Spaß an Bewegung finden – Menschen mit chronischen Schmerzen erscheint dies am Anfang der Therapie oft schwer erreichbar. In der
Bewegungsgruppe Schmerzstörung nähern sich Patienten diesem Ziel mithilfe einer psychosomatischen Schmerzphysiotherapie Schritt für
Schritt an. Zunächst gilt es, wieder Vertrauen in den eigenen Körper zu gewinnen.
Wissenschaft und Praxis
Authors: Reichert Bernhard , Egger Bernd 
Published: November 2017

ZusammenfassungDie Bedeutung von Forschungswissen im physiotherapeutischen Alltag untersuchte eine Online-Befragung deutschsprachiger Therapeuten. Die
Teilnehmer informieren sich regelmäßig über Forschungswissen in elektronischen Medien. Jedoch setzt sich nur eine (studierte) Minderheit mit
verschriftlichtem Wissen auseinander.
Perioperative Infusionstherapie bei Kindern – die neue S1-Leitlinie
Authors: Sümpelmann Robert 
Published: November 2017

ZusammenfassungIn der neuen S1-Leitlinie 1 wurden die Handlungsempfehlungen des wissenschaftlichen Arbeitskreises Kinderanästhesie (WAKKA) von 2006 2 durch eine repräsentativ zusammengesetzte Expertengruppe aktualisiert und nach dem Regelwerk der Arbeitsgemeinschaft der Wissenschaftlichen Medizinischen Fachgesellschaften (AWMF) überarbeitet 3.
Brillengläser – eine Übersicht
Authors: Buser Annemarie , Michels Ralf , Baumbach Peter 
Published: November 2017

ZusammenfassungDie Frage, welches das richtige Brillenglas sei, lässt sich nicht eindeutig beantworten, die Antwort ist von vielen Faktoren abhängig. Je nach Sehanforderungen gibt es nicht ein richtiges Brillenglas, sondern es muss ein Kompromiss zwischen verschiedenen Eigenschaften gefunden werden. Dieser Artikel möchte eine Hilfestellung bei der Beantwortung der Frage nach dem für den individuellen Patienten bzw. für die jeweilige Anforderung optimalen Brillenglas geben.
Arterielle Gefäßverschlüsse der Retina
Authors: Lang Gabriele , Lang Stefan 
Published: November 2017

ZusammenfassungRetinale Gefäßverschlüsse sind häufig durch eine Embolisation, seltener durch eine Thrombose im Rahmen einer Arteriitis bedingt. In der Akutphase kann die Diagnose meist klinisch gestellt werden, Schwierigkeiten bereitet sie jedoch in der chronischen Erkrankungsphase. Die diagnostischen und therapeutischen Möglichkeiten beschreibt dieser Beitrag. Wichtig ist die Abklärung möglicher Grunderkrankungen, um z. B. kardiovaskuläre Ereignisse zu verhindern.
Die neuroophthalmologische Anamnese
Authors: Wilhelm Helmut 
Published: November 2017

ZusammenfassungNeuroophthalmologische Anamnesen können sehr umfangreich und schwierig sein. Dieser Beitrag gibt 14 Ratschläge, wie man die Anamnese möglichst effizient gestalten und das Ansammeln irrelevanter Informationen vermeiden kann.
Additive effects of climate and fisheries drive ongoing declines in multiple albatross species
Authors: Pardo Deborah , Forcada Jaume , Wood Andrew G. , Tuck Geoff N. , Ireland Louise , Pradel Roger , Croxall John P. , Phillips Richard A. 
Published: November 2017

Significance
Three high-conservation priority populations were studied: the wandering, grey-headed, and black-browed albatrosses from Bird Island, South Georgia. They represent 12–50% of global numbers and have declined by 40–60% in 35 years. As temperatures and environmental stochasticity increase, polar species are particularly at risk, while fisheries accidentally kill hundreds of thousands individuals each year. Longitudinal monitoring of >40,000 individuals ringed since 1972 was used with detailed at-sea distributions, environmental data, and fisheries effort spanning the Southern Ocean to explore the factors driving population change and how they may combine. The powerful comparative framework used here is one of the most extensive to date and could be used to understand and better mitigate the fate of many threatened wild populations.
Kinship structures create persistent channels for language transmission
Authors: Lansing J. Stephen , Abundo Cheryl , Jacobs Guy S. , Guillot Elsa G. , Thurner Stefan , Downey Sean S. , Chew Lock Yue , Bhattacharya Tanmoy , Chung Ning Ning , Sudoyo Herawati , Cox Murray P. 
Published: November 2017

SignificanceAssociations between genes and languages occur even with sustained migration among communities. By comparing phylogenies of genes and languages, we identify one source of this association. In traditional tribal societies, marriage customs channel language transmission. When women remain in their natal community and men disperse (matrilocality), children learn their mothers’ language, and language correlates with maternally inherited mitochondrial DNA. For the converse kinship practice (patrilocality), language instead correlates with paternally inherited Y chromosome. Kinship rules dictating postmarital residence can persist for many generations and determine population genetic structure at the community scale. The long-term association of languages with genetic clades created by kinship systems provides information about language transmission, and about the structure and persistence of social groups.
CD1b-restricted GEM T cell responses are modulated byMycobacterium tuberculosismycolic acid meromycolate chains
Authors: Chancellor Andrew , Tocheva Anna S. , Cave-Ayland Chris , Tezera Liku , White Andrew , Al Dulayymi Juma’a R. , Bridgeman John S. , Tews Ivo , Wilson Susan , Lissin Nikolai M. , Tebruegge Marc , Marshall Ben , Sharpe Sally , Elliott Tim , Skylaris Chris-Kriton , Essex Jonathan W. , Baird Mark S. , Gadola Stephan , Elkington Paul , Mansour Salah 
Published: November 2017

SignificanceTuberculosis is a major global pandemic responsible for more deaths than any other infectious disease, yet no effective vaccine exists. Here, we demonstrate CD1b expression within human tuberculous granulomas, supporting a role for CD1b lipid antigen presentation in host immunity to infection. CD1b presents mycolates, the dominantMycobacterium tuberculosis(Mtb) cell wall lipid class and key virulence factors, to αβ T cells. We reveal that mycolate tail moieties, distal to the head group, are antigenic determinants for the conserved human germline-encoded mycolyl lipid-reactive (GEM) T cell receptors (TCRs). Computational simulations suggest a putative mechanism whereby lipid-ligand dynamics within CD1b regulate GEM-TCR activity. This work provides insights for the development of major histocompatibility complex (MHC)-independent Mtb lipid vaccines, including those that target GEM T cells.
Holocene fluctuations in human population demonstrate repeated links to food production and climate
Authors: Bevan Andrew , Colledge Sue , Fuller Dorian , Fyfe Ralph , Shennan Stephen , Stevens Chris 
Published: November 2017

Significance
The relationship between human population, food production, and climate change is a pressing concern in need of high-resolution, long-term perspectives. Archaeological radiocarbon dates have increasingly been used to reconstruct past population dynamics, and Britain and Ireland provide both radiocarbon sampling densities and species-level sample identifications that are globally unrivalled. We use this evidence to demonstrate multiple instances of human population downturn over the Holocene that coincide with periodic episodes of reduced solar activity and climate reorganization as well as societal responses in terms of altered food-procurement strategies.
Dynamics of a rolling robot
Authors: Ilin K. I. , Moffatt H. K. , Vladimirov V. A. 
Published: November 2017

Significance
The dynamics of a rolling ball activated by an internal battery mechanism are analyzed by theoretical and numerical techniques. The problem involves four independent dimensionless parameters and is governed by a six-dimensional nonholonomic nonautonomous dynamical system with cubic nonlinearity. It can serve as a prototype for rolling bodies activated by any internal mechanism and is relevant to robotic systems for which such an internal mechanism may be subject to remote control. This is believed to be a unique problem of its kind to have been solved by appeal to fundamental principles of classical dynamics. For this reason, it should be accessible to a wide readership. The numerical results provide clear evidence of both regular and chaotic behavior.
Long-term dopamine neurochemical monitoring in primates
Authors: Schwerdt Helen N. , Shimazu Hideki , Amemori Ken-ichi , Amemori Satoko , Tierney Patrick L. , Gibson Daniel J. , Hong Simon , Yoshida Tomoko , Langer Robert , Cima Michael J. , Graybiel Ann M. 
Published: November 2017

Significance
Dopamine is an important neurotransmitter governing behavior and heavily implicated in a large range of neural disorders, including Parkinson’s disease, depression, and related mood and movement disorders. Methods allowing accurate monitoring of dopamine over long timescales have not been previously reported yet are crucial to enable improved diagnostics and therapeutics. We report a technical advance that allowed recording of dopamine from sensors implanted into the brains of nonhuman primates for over 100 days. Our integrated platform enabled monitoring fast changes in dopamine release in response to rewarding stimuli and the administration of dopamine-related drugs. These findings demonstrate the long-term feasibility and reproducibility of neurochemical measurements and strengthen their potential translation to human use.