bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vgll2 as an integrative regulator of mitochondrial function and contractility specific to skeletal muscle
Authors: Honda Masahiko , Inoue Ryota , Nishiyama Kuniyuki , Ueda Takeshi , Komuro Akiyoshi , Amano Hisayuki , Sugisawa Ryoichi , Dash Suman , Shirakawa Jun , Okada Hitoshi 
Published: September 2024

AbstractDuring skeletal muscle adaptation to physiological or pathophysiological signals, contractile apparatus and mitochondrial function are coordinated to alter muscle fiber type. Although recent studies have identified various factors involved in modifying contractile proteins and mitochondrial function, the molecular mechanisms coordinating contractile and metabolic functions during muscle fiber transition are not fully understood. Using a gene‐deficient mouse approach, our previous studies uncovered that vestigial‐like family member 2 (Vgll2), a skeletal muscle‐specific transcription cofactor activated by exercise, is essential for fast‐to‐slow adaptation of skeletal muscle. The current study provides evidence that Vgll2 plays a role in increasing muscle mitochondrial mass and oxidative capacity. Transgenic Vgll2 overexpression in mice altered muscle fiber composition toward the slow type and enhanced exercise endurance, which contradicted the outcomes observed with Vgll2 deficiency. Vgll2 expression was positively correlated with the expression of genes related to mitochondrial function in skeletal muscle, mitochondrial DNA content, and protein abundance of oxidative phosphorylation complexes. Additionally, Vgll2 overexpression significantly increased the maximal respiration of isolated muscle fibers and enhanced the suppressive effects of endurance training on weight gain. Notably, no additional alteration in expression of myosin heavy chain genes was observed after exercise, suggesting that Vgll2 plays a direct role in regulating mitochondrial function, independent of its effect on contractile components. The observed increase in exercise endurance and metabolic efficiency may be attributed to the acute upregulation of genes promoting fatty acid utilization as a direct consequence of Vgll2 activation facilitated by endurance exercise. Thus, the current study establishes that Vgll2 is an integrative regulator of mitochondrial function and contractility in skeletal muscle.
Impact of an immersive engineering program on children's understanding of and interest in engineering: Addressing gender stereotypes
Authors: Schonning Alexandra , Perez Susan M. 
Published: September 2024

AbstractBackgroundThe gender gap in engineering presents a critical barrier to achieving a diverse and innovative engineering workforce. This gap is influenced by gender stereotypes, socialization processes, masculine culture, and insufficient early experiences with engineering. These contribute to diminished STEM self‐concept, interest, and participation among women.Purpose/HypothesisThis study assessed an engineering outreach program's impact on children's understanding of and interest in engineering and beliefs about who can be an engineer. It compared whether discussions of gender bias differentially influenced these outcomes for girls and boys.Design/MethodThe study was a short‐term sequential quasi‐experimental design with boys and girls in grades 4–5. Six classroom groups attended a half‐day engineering field trip and were randomly assigned to two conditions: Gender Presentation or No Gender Presentation. Pre‐ and post‐program surveys measured changes in understanding of and interest in engineering and beliefs about who can be an engineer.ResultsAll children, regardless of gender or condition, had higher understanding of and interest in engineering after the program, but also demonstrated a bias in selecting men over women as engineers. Participants reported that both girls and boys like and are good at engineering and these ratings increased from the pre‐program to the post‐program survey.ConclusionThis research affirms the effectiveness of immersive and inclusive programming in enhancing children's understanding of and interest in engineering. No substantial gender‐specific differences emerged regarding the impact of discussions on gender biases. The program underscores the value of early inclusive educational interventions in fostering equitable interest in engineering among young students.
Clavicular tunnel widening in chronic acromioclavicular joint instabilities after primary versus revision arthroscopically‐assisted acromio‐ and coracoclavicular stabilization
Authors: Vetter Philipp , Bellmann Frederik , Eckl Larissa , Lazaridou Asimina , Scheibel Markus 
Published: September 2024

AbstractPurposeTo evaluate joint reduction (loss of reduction [LOR]; dynamic posterior translation [DPT]) and clavicular tunnel widening (cTW) in patients treated with arthroscopically‐assisted acromioclavicular joint (ACJ) stabilization after previously failed nonoperative versus surgical treatment.MethodsPatients undergoing arthroscopically‐assisted ACJ stabilization (bidirectional tendon allograft with a low‐profile TightRope) after previously failed nonoperative versus surgical treatment were included retrospectively. Bilateral anteroposterior stress views served for evaluating LOR (side‐comparative coracoclavicular distance [CCD]) and cTW at a 6‐weeks‐ and 6‐months‐follow‐up (FU) and for evaluating the filling ratio (FR, vertical device insertion depth relative to clavicle height) at the 6‐weeks‐FU. Postoperative DPT was assessed on Alexander's views.ResultsTwenty‐seven patients (20 male, mean age 46.1 ± 14.8 years) were included (prior treatment: nonoperative: n = 15; surgical: n = 12). There were no differences in LOR, DPT or cTW between groups postoperatively. Initial CCD‐symmetry at the 6‐weeks‐FU (CCD: −0.1 mm [95% confidence interval, CI, −2 to 1.4 mm]) was followed by LOR at the 6‐months‐FU (CCD: −3.5 mm [95% CI, −5.2 to −1.9 mm]; p < 0.001). cTW increased towards the inferior cortex, compared to the superior cortex and the intermediate level (p < 0.001, respectively). cTW at the inferior cortex was associated with more LOR (r = −0.449; p = 0.024) and DPT (r = 0.421; p = 0.036), dependent on a smaller FR (r = −0.430; p = 0.032).ConclusionPatients undergoing arthroscopically‐assisted ACJ stabilization for chronic bidirectional ACJ instabilities showed comparable radiologic results after previous nonoperative versus surgical treatment. cTW increased towards the inferior cortex and was associated with recurrent vertical and horizontal instability, related to a smaller FR. More research into reduced cTW, for example, by a more filling device, should be performed.Level of EvidenceLevel III, retrospective comparative study.
Enhancing patient information texts in orthopaedics: How OpenAI's ‘ChatGPT’ can help
Authors: Yüce Ali , Yerli Mustafa , Misir Abdulhamit , Çakar Murat 
Published: September 2024

AbstractPurposeThe internet has become a primary source for patients seeking healthcare information, but the quality of online information, particularly in orthopaedics, often falls short. Orthopaedic surgeons now have the added responsibility of evaluating and guiding patients to credible online resources. This study aimed to assess ChatGPT's ability to identify deficiencies in patient information texts related to total hip arthroplasty websites and to evaluate its potential for enhancing the quality of these texts.MethodsIn August 2023, 25 websites related to total hip arthroplasty were assessed using a standardized search on Google. Peer‐reviewed scientific articles, empty pages, dictionary definitions, and unrelated content were excluded. The remaining 10 websites were evaluated using the hip information scoring system (HISS). ChatGPT was then used to assess these texts, identify deficiencies and provide recommendations.ResultsThe mean HISS score of the websites was 9.5, indicating low to moderate quality. However, after implementing ChatGPT's suggested improvements, the score increased to 21.5, signifying excellent quality. ChatGPT's recommendations included using simpler language, adding FAQs, incorporating patient experiences, addressing cost and insurance issues, detailing preoperative and postoperative phases, including references, and emphasizing emotional and psychological support. The study demonstrates that ChatGPT can significantly enhance patient information quality.ConclusionChatGPT's role in elevating patient education regarding total hip arthroplasty is promising. This study sheds light on the potential of ChatGPT as an aid to orthopaedic surgeons in producing high‐quality patient information materials. Although it cannot replace human expertise, it offers a valuable means of enhancing the quality of healthcare information available online.Level of EvidenceLevel IV.
Edge‐transitive cubic graphs of twice square‐free order
Authors: Liu Gui Xian , Lu Zai Ping 
Published: September 2024

AbstractA graph is edge‐transitive if its automorphism group acts transitively on the edge set. This paper presents a complete classification for connected edge‐transitive cubic graphs of order , where is even and square‐free. In particular, it is shown that such a graph is either symmetric or isomorphic to one of the following graphs: a semisymmetric graph of order 420, a semisymmetric graph of order 29,260, and five families of semisymmetric graphs constructed from the simple group .
In the attic of dreams. The personal archives of the father of paradoxical sleep
Authors: Roelli Michael 
Published: September 2024

AbstractMichel Jouvet (1925–2017) is one of the most important figures in the contemporary history of the neuroscience of sleep and dreams, and one of the most awarded French researchers of the last century. Yet this former CNRS gold medalist and winner of the Cino Del Duca World Prize remains little known—not to say unknown—outside the field of sleep medicine, especially in non‐French‐speaking countries, where the name of his American counterpart, William C. Dement, is more familiar. Often reduced to his experiments on cats and the discovery of what he called “paradoxical sleep,” Jouvet left behind a rather unique body of work that includes not only countless publications on sleep and dreams—neurophysiological as well as ethnological and psychological—but also major contributions to clinical medicine, two novels and an impressive collection of personal dream accounts and drawings, which now make it possible to explore the nocturnal side of the last 50 years of his life. This article draws on unpublished archives to illuminate all these little‐known and unknown aspects of Jouvet's life and work, highlighting his hidden links with 19th‐century scientific oneirology and bringing to light its paradoxes.
Multicomponent Assembling of Aldehydes, N,N‐Dimethylbarbituric Acid, Malononitrile, and Morpholine Into Unsymmetrical Ionic Scaffold and Its Efficient Cyclization
Authors: Elinson Michail N. , Ryzhkova Yuliya E. , Kalashnikova Varvara M. , Chizhov Alexander O. , Egorov Mikhail P. 
Published: September 2024

ABSTRACTThe new type of the four‐component tandem Knoevenagel–Michael reaction was discovered: the transformation of arylaldehydes, N,N′‐dimethylbarbituric acid, malononitrile, and morpholine in alcohols and other organic solvents without any other additives at ambient temperature resulted in the selective formation of a new substituted unsymmetrical ionic scaffold with two pharmacology‐active heterocyclic rings. This new four‐component reaction is a simple and efficient route to a previously unknown substituted ionic unsymmetrical scaffold containing N,N′‐dimethylbarbituric acid and morpholine. These ionic scaffolds are promising for various biomedical applications, for example, as anticonvulsants and anti‐inflammatory drugs, as well as anti‐AIDS agents. In this research, we also accomplished the efficient cyclization of morpholin‐4‐ium 5‐(2,2‐dicya‐no‐1‐arylethyl)‐1,3‐dimethyl‐2,6‐di‐oxo‐1,2,3,6‐tetrahydro‐pyrimidin‐4‐olates by the action of NBS–NaOAc system, with the formation of 5,7‐dimethyl‐4,6,8‐trioxo‐2‐aryl‐5,7‐diazaspiro[2.5]octane‐1,1‐dicarbonitriles in 83%–98% yields. In the final stage of our research, direct one‐pot multicomponent transformation of benzaldehydes, N,N′‐dimethylbarbituric acid, and malononitrile into spirobarbituric cyclopropanes was accomplished in 75%–97% yields. The spiro‐barbituric cyclopropanes are potentially active as remedies against various inflammatory, infectious, immunological, or malignant diseases.
Compact and low‐loss IPD bandpass filter using 3D glass‐based redistribution layer technology
Authors: Li HangXing , Cao Yazi , Zhao Peng , Wang Gaofeng 
Published: September 2024

AbstractA low‐loss miniaturized bandpass filter is presented using 3D glass‐based RDL packaging technology. A new topology consisting of one modified Pi‐section and one modified T‐section is introduced to generate three transmission zeros which can achieve the high out‐of‐band rejection. A combination of 2D planar inductors and high‐Q 3D inductors is used to achieve low insertion loss and minimize the filter size. In addition, one grounded resonator at input terminal is introduced to generate an extra transmission zero in the low end. The proposed bandpass filter covering 3.3–4.2 GHz is fabricated with a compact size of 1.6 mm × 0.8 mm × 0.25 mm. It exhibits an insertion loss of less than 1.0 dB at a center frequency of 3.75GHz and a return loss of better than 14 dB. The proposed design has a 3 dB fractional bandwidth of 37.6%. Its out‐of‐band rejection is better than 20 dB at the low frequency band from DC to 2.0 GHz and better than 19 dB at the high frequency band from 5.0 to 9.0 GHz. The simulated and measured results of the proposed BPF are in reasonably good agreement.
Design and performance analysis of compact quad‐band two‐port sickle‐shaped MIMO antenna for wireless applications
Authors: Yadav Ashok , Singh Pramod , Bali Vikram , Kumar Akhilesh , Pattanayak Prabina , Verma Ramesh Kumar 
Published: September 2024

AbstractIn this research, a quad‐band sickle‐shaped MIMO antenna of compact size 32 × 32 mm2 (0.28λ0 × 0.28λ0 at lower resonant frequency 2.6 GHz) has been proposed using partial ground and tilted isolation stub at an angle of 45°. It covers 2.40 to 2.80 GHz frequency range with bandwidth (BW) of 15.38% (0.40 GHz) which is applicable for Wi‐Fi application in first band and in second band it covers from 4.48 to 5.75 GHz frequency range with BW of 24.83% (1.27 GHz) for WLAN application. In addition, the third band starts from 9.75 to 10.43 GHz frequency range with BW of 6.74% (0.68 GHz) for the X‐band whereas in fourth band it covers from 13.07 to 15.87 GHz frequency range with BW of 19.38% (2.8 GHz) frequency, which is applicable on Ku‐band application. Moreover, the isolation is < −15 dB in the entire resonating frequency band which is an acceptable limit for reduction of mutual coupling. ECC value is <0.5 in the entire operating band of frequency. The diversity gain of the proposed MIMO antenna is approximately 9.96 dB that shows the antenna exhibits good diversity property. The channel capacity loss value is <0.4 bps/s/Hz in the entire resonating band. In view of all the diversity parameters, the proposed MIMO structure fulfills all the requirements of the MIMO antenna.
Sensorimotor Synchronization and Neural Entrainment to Imagined Rhythms in Individuals With Proficient Imagery Ability
Authors: Whitton Simon Andrew , Sreenan Benjamin , Luo Canhuang , Jiang Fang 
Published: September 2024

ABSTRACTSensorimotor synchronization (SMS) is the temporal coordination of motor movements with external or imagined stimuli. Finger‐tapping studies indicate better SMS performance with auditory or tactile stimuli compared to visual. However, SMS with a visual rhythm can be improved by enriching stimulus properties (e.g., spatiotemporal content) or individual differences (e.g., one's vividness of auditory imagery). We previously showed that higher self‐reported vividness of auditory imagery led to more consistent synchronization–continuation performance when participants continued without a guiding visual rhythm. Here, we examined the contribution of imagery to the SMS performance of proficient imagers, including an auditory or visual distractor task during the continuation phase. While the visual distractor task had minimal effect, SMS consistency was significantly worse when the auditory distractor task was present. Our electroencephalography analysis revealed beat‐related neural entrainment, only when the visual or auditory distractor tasks were present. During continuation with the auditory distractor task, the neural entrainment showed an occipital electrode distribution, suggesting the involvement of visual imagery. Unique to SMS continuation with the auditory distractor task, we found neural and sub‐vocal (measured with electromyography) entrainment at the three‐beat pattern frequency. In this most difficult condition, proficient imagers employed both beat‐ and pattern‐related imagery strategies. However, this combination was insufficient to restore SMS consistency to that observed with visual or no distractor task. Our results suggest that proficient imagers effectively utilized beat‐related imagery in one modality when imagery in another modality was limited.
An Additional L451G452N453 in the RpoB Protein Suppressed the Synthetic Lethality in Escherichia coli at 37 Degrees Caused by Depletion of DnaK/J and Trigger Factor
Authors: Fan Dongjie , Liu Lushan , Yuen Bella , Sun Lu , Fu Yuliang , Liu Yan , Liao Rui , Qu Yanli , Liu Chuanpeng , Zhou Qiming 
Published: September 2024

ABSTRACTEscherichia coli depletion of chaperone trigger factor and DnaK/J were not viable at 37°C, but viable below 30°C. Among the engineered E. coli depleted of trigger factor and DnaK/J, one strain Z625, exhibited survival at 37°C, while another strain Z629 only survived below 30°C. Comparative analysis of fatty acid profiles of Z625 and Z629 revealed absence of numerous saturated fatty acids in Z625 as compared to the wild‐type E. coli BW25113. In addition, increased unsaturated fatty acids were present in Z625, whereas the fatty acids profile of Z629 closely resembled that of BW25113. Whole genome sequencing revealed a 9‐bp insertion in rpoB of Z625. Combined structural analysis of simulated RpoB protein bearing the amino acid sequence L451G452N453 insertion and susceptibility analysis to rifampicin suggested that the insertion did not disturb the individual RpoB structure as beta subunit of RNA polymerase. Comparative transcriptomic analysis of Z625 and Z629 suggested that this insertion impacted transcription of the overall RNA polymerase in Z625, leading to potential repression of some genes whose overexpression was toxic to E. coli. Additionally, Z625 exhibited distinctive metabolic adaptations, likely contributing to its survival at 37°C. In summary, our study elucidated one LGN insertion in rpoB that impacts transcriptional regulation in E. coli, thereby explaining the survival of E. coli depletion of trigger factor and DnaK/J at 37°C, and these founding suggested that some simple mutations in critical genes like rpoB might play an important role in driving adaptive evolution.
The Ecological Strategy Determines the Response of Fungi to Stress: A Study of the 2,4‐diacetylphloroglucinol Activity Against Aspergillus and Fusarium Species
Authors: Stepanov Artyom A. , Shulaev Nikita A. , Vasilchenko Alexey S. 
Published: September 2024

ABSTRACTAspergillus and Fusarium are two economically important genera of fungi. They cause significant yield losses and contamination of crops with mycotoxins. In this study we aimed to evaluate the impact of 2,4‐diacetylphloroglucinol (2,4‐DAPG) on Aspergillus and Fusarium fungi. It is hypothesized that two fungal genera, which have different ecological strategies, react differently to stress caused by a secondary metabolite produced by rhizosphere Pseudomonas species. We found that 2,4‐DAPG was able to reduce biofilm formation of Aspergillus and Fusarium, as reflected in biomass and its chemical composition. Furthermore, subinhibitory concentrations of 2,4‐DAPG increased the levels of ergosterol and polysaccharides (α‐ and β‐glucans, chitin) in the cell membrane and cell wall of Aspergillus, while decreasing them in Fusarium. 2,4‐DAPG altered the production of secondary metabolites, especially mycotoxins and extracellular proteases. The production of ochratoxin A was decreased in A. ochraceus, and T‐2 toxin and zearalenone, on the contrary, were increased in F. culmorum and F. sporotrichioides, respectively. Thus, using 2,4‐DAPG we demonstrated that the ecological role of fungi determines their reaction to antibiotic substances produced by the plant microbiome. Our data contributes to understanding the molecular mechanisms behind symbiotic relationships in natural communities, which are mediated by the biosynthesis of antibiotics.
Effect of refrigeration and reheating on the lipid oxidation and volatile compounds in silver carp surimi gels
Authors: Huang Jingjing , Yin Tao , Xiong Shanbai , Huang Qilin 
Published: September 2024

AbstractBACKGROUNDAs unsaturated and saturated aldehydes, ketones are known to be responsible for off‐odors in surimi products, and they are mainly derived from lipid oxidation. Because surimi‐based products are rich in unsaturated fatty acids, they are prone to producing off‐odors during the refrigeration and reheating processes, which are common treatments for leftovers. The present study investigated the color, lipid oxidation productions, fatty acid profiles and volatile components in surimi gels during refrigeration at 4 °C for 3 days with multiple reheating.RESULTSThe results revealed that the accumulation rate of hydroperoxides was higher in the refrigeration stage, whereas the decomposition rate was higher during reheating in surimi gels. Both refrigeration and reheating treatments promoted conjugated diene values, acid values and carbonyl values. Nevertheless, reheating treatment decreased tohiobarbituric acid reactive substances and whiteness. The contents of unsaturated fatty acids, especially α‐linolenic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid, were reduced, whereas the contents of saturated fatty acids increased during refrigeration and multiple reheating. The unsaturated fatty acids were lost as a result of their oxidative deterioration. The volatile components profile showed that the accumulation of volatile components mainly occurred in the refrigeration stage. Multivariate data analysis was utilized to further clarify whether the off‐odors in surimi gels were mainly generated in refrigeration.CONCLUSIONRefrigeration and reheating both contributed to lipid oxidation and the generation of volatile compounds in surimi gels, but the off‐odors were mainly generated during refrigeration. This research provides a novel understanding of the formation of food odors in processing. © 2024 Society of Chemical Industry.