bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic insights and functional evaluation of Lacticaseibacillus paracasei EG005: a promising probiotic with enhanced antioxidant activity
Authors: Kim Jisu , Jo Jinchul , Cho Seoae , Kim Heebal 
Published: October 2024

IntroductionProbiotics, such as Lacticaseibacillus paracasei EG005, are gaining attention for their health benefits, particularly in reducing oxidative stress. The goal of this study was to reinforce the antioxidant capacity of EG005, along with comprehensive genomic analysis, with a focus on assessing superoxide dismutase (SOD) activity, acid resistance and bile tolerance, and safety.MethodsEG005 was screened for SOD activity and change of SOD activity was tested under various pH conditions. Its survival rates were assessed in acidic (pH 2.5) and bile salt (0.3%) conditions and the antibiotic MIC test and hemolysis test were performed to evaluate safety. Genetic analyses including functional identification and phylogenetic tree construction were performed. The SOD overexpression system was constructed using Ptuf, Pldh1, Plhd2, and Pldh3 strong promoters.ResultsEG005 demonstrated higher SOD activity compared to Lacticaseibacillus rhamnosus GG, with optimal activity at pH 7.0. It showed significant acid and bile tolerance, with survival rates recovering to 100% after 3 h in acidic conditions. Phylogenetic analysis confirmed that EG005 is closely related to other L. paracasei strains with ANI values above 98%. Overexpression of SOD using the Ptuf promoter resulted in a two-fold increase in activity compared to the controls. Additionally, EG005 exhibited no hemolytic activity and showed antibiotic susceptibility within safe limits.DiscussionOur findings highlight EG005’s potential as a probiotic with robust antioxidant activity and high tolerance to gastrointestinal conditions. Its unique genetic profile and enhanced SOD activity through strong promoter support its application in probiotic therapies and functional foods. Further research should be investigated to find the in vivo effects of EG005 on gut health and oxidative stress reduction. In addition, attB and attP-based recombination, combined with CRISPR-Cas9 technologies, could offer a more stable alternative for long-term sodA gene expression in commercial and medical applications.
Breast cancer and neoplasms of the thyroid gland: a bidirectional two-sample Mendelian randomization study
Authors: Sun Yiqi , Wan Bohan , Liu Xin , Dong Jianguo , Yin Shengjie , Wu Yiqi 
Published: October 2024

BackgroundWith the rising incidence of breast cancer (BC) and neoplasms of the thyroid gland, a potential link between the two has drawn increasing attention. However, the causal relationship remains unclear due to various confounding factors. This study aims to investigate the causality between BC and thyroid tumors using Mendelian Randomization (MR) analysis.MethodsWe conducted a bidirectional two-sample MR analysis, utilizing breast cancer-associated single nucleotide polymorphisms (SNPs) from the Breast Cancer Association Consortium (BCAC) and thyroid tumor-related SNPs from the FinnGen (https://www.finngen.fi/) database. First, we performed univariable MR (UVMR) to assess the causal relationship between BC and both malignant and benign thyroid tumors, followed by reverse causality analysis. To account for potential confounders, we applied multivariable MR (MVMR). The inverse-variance weighted (IVW) method was primarily used, with secondary analyses performed using the weighted median and MR-Egger regression approaches.ResultsUVMR analysis revealed a significant positive causal relationship between BC and malignant thyroid tumors (odds ratio [OR] and 95% confidence interval [CI]: 1.291, 1.143–1.458, P = 3.90×10-5). No causal relationship was found between BC and benign thyroid tumors. The MVMR analysis, adjusting for confounding factors such as smoking, drinking, and body mass index (BMI), confirmed the robustness of the results.ConclusionThis study provides genetic evidence supporting a causal relationship between BC and malignant thyroid tumors. These findings highlight the importance of thyroid cancer screening in BC patients. However, further MR studies or randomized controlled trials (RCTs) are necessary to assess small effects accurately.
Self-regulation and performance among elite youth soccer players: the role of approach-avoidance motivation
Authors: Hamoud Mounir , Sæther Stig Arve , Bjørnebekk Gunnar 
Published: October 2024

Introduction and methodsThis study aimed to investigate the motivational processes behind self-regulated learning and performance among 192 soccer players (82 girls) for three age groups (14–16 years old) eligible for the Norwegian national football team. A conditional process model was proposed and tested with achievement goals as mediators between achievement motives on the one hand and coach-reported performance and self-regulated learning on the other hand. The probability of success was examined as a potential moderator in the motivational process.ResultsAs predicted, motives to achieve success directly influenced planning and reflection/evaluation, whereas the influence of the success motive on regulation of effort was explained partly by task- and self-based approach goals. The motive to achieve success was, however, particularly crucial for maintaining these beneficial regulatory processes when the probability of success was found to be from moderate to low. Concerning the avoidance paths, the data supported only some of our original hypotheses. The motive to avoid failure predicted all three types of avoidance-based (task, self, and other) and other-based approach goals but did not contribute to explaining planning, reflection/evaluation, regulation of effort, or performance. There were no significant correlations between motivation variables and coach-reported performance. Moreover, girls were more motivated to avoid failure than boys, while both sexes achieved similar scores for football-specific self-regulated learning, probability of success, achievement goals, and motive to achieve success.DiscussionThe results are discussed considering a hierarchical motivation model.
“I feel so small and big at the same time”—exploring body experience and binge eating disorder following inpatient treatment: a qualitative study
Authors: Olsen Helene T. , Vangen Sunniva B. , Stänicke Line Indrevoll , Vrabel KariAnne 
Published: October 2024

BackgroundLimited research into binge eating disorder (BED), a low treatment rate, and a lack of treatment rights, reflects a marginalized disorder in society and a treatment context.AimThe aim of this study was to gain a deeper understanding of the psychopathology of BED, by exploring the patients’ meanings related to the disorder and the role of the body in the treatment of BED.MethodQualitative methodology using a reflexive thematic analysis. The data material is based on semi-structured in-depth interviews with six cis-gendered women who had previously completed an inpatient treatment program for BED at a department for EDs and met the criteria for 307.51 Binge Eating Disorder in DSM-5.ResultsThe qualitative analysis rendered two meta-themes comprising six themes and 12 subthemes. The initial meta-theme was “Relational challenges and feelings could not be talked about at home.” The secondary meta-theme was “Body contempt disturbs the experience of self and others” and the third was “Their body has not been a theme in previous treatment.” This categorization depicts a narrative of the disorder in terms of development, current condition, and experiences with prior treatment.DiscussionThe findings indicate that shame and disgust are central to playing pivotal roles in the embodiment of BED, highlighting the significance of addressing one’s relationship with their body for achieving recovery and preventing an experienced repetition of the perceived rejection as a child.
Weathering words: a virtual reality study of environmental influence on reading dynamics
Authors: Rocabado Francisco , Muntini Laís , González Alonso Jorge , Duñabeitia Jon Andoni 
Published: October 2024

IntroductionReading is a fundamental cognitive activity that is influenced by both textual and external environmental factors, although the latter has been less thoroughly explored. This study aims to examine the impact of environmental visual conditions on reading performance using Virtual Reality (VR) technology.MethodsWe conducted two experiments to assess the effects of visual contrast and simulated weather conditions on reading dynamics. In Experiment 1, we measured single-word recognition speed using a lexical decision task under different visual contrasts and weather conditions. In Experiment 2, we assessed reading dynamics during a sentence reading task, analyzing how visual contrast and simulated sunny versus rainy weather conditions affected reading behavior, particularly focusing on reading speed and eye fixations.ResultsIn Experiment 1, high visual contrast, particularly under sunny conditions, significantly enhanced single-word recognition speed, indicating a notable influence of environmental visual conditions. In Experiment 2, visual contrast had minimal effect on sentence reading; however, sunny weather facilitated faster reading times, while rainy scenarios increased the number of eye fixations.DiscussionThese findings suggest that environmental factors, such as weather conditions, can significantly affect reading behavior. The study contributes to the understanding of key environmental influences on reading in everyday life contexts and has implications for the ergonomic design of reading materials, especially for outdoor settings and VR environments. Additionally, the integration of controlled stimuli within VR increases the ecological validity of reading research, underscoring the potential of VR as a powerful tool for cognitive research.
Improving mental health among intensive care unit staff with communication skills training
Authors: Holmberg Johan , Rosendahl Ingvar , Andersson Rebecca , Kemani Mike K. , Holmström Linda , Öst Lars-Göran , Wicksell Rikard K. 
Published: October 2024

PurposeIntensive Care Unit (ICU) staff report that a large part of the work-related distress they experience is related to communication situations with colleagues, patients, and relatives. Based on these findings, the aim of the present study was to preliminary evaluate the effects of a novel Communication Skills Training (CST) program on mental health among ICU staff.MethodsThe CST program was delivered to the entire work force of an ICU at a Swedish hospital and was evaluated as an uncontrolled clinical trial with three repeated measures. 100 participants were eligible and included in the analyses. The theoretical framework for the program was learning theory and cognitive behavioral therapy. The program was delivered by two psychologists and included one full-day lecture and three subsequent two-hour supervision sessions. Changes in mental health was evaluated with self-report questionnaires measuring perceived stress, general mental health, work engagement, and psychological flexibility.ResultsAnalyses of three repeated measures showed significant improvements with 1.3 points lower perceived stress and 2.0 points lower general mental health after the intervention. No differences were seen in measures of work engagement or psychological flexibility.ConclusionFindings of the study supports the utility of the CST program as an intervention to target perceived stress and general mental health within intensive care. These findings should be further validated in trials with improved design.
Institutional investment in addictive industries: an important commercial determinant of health
Authors: Berret Sébastien , Marionneau Virve , Sievänen Riikka , Nikkinen Janne 
Published: October 2024

BackgroundThe production of addictive commodities is subject to a range of commercial determinants. There has nevertheless been a gap in understanding how investments into addictive commodities may function as commercial determinants. Institutional investors can yield important financial power with their investment decisions. Many investors apply responsible investment (RI) policies to address environmental, social, and governance concerns. Negative screening is used to exclude certain sectors or companies from investment portfolios, mainly for ethical concerns. Negative screening also affects investment into tobacco and other addictive industries. This article investigates RI policies toward addictive industries among institutional investors that are signatories of the Tobacco-Free Finance Pledge (TFFP; N = 161). The TFFP is an initiative created in 2021 to de-normalize tobacco-related investments.MethodsThe mixed-method study uses descriptive statistics to quantify the extent and scope of exclusion policies as well as institutional and geographical profiles of investors, and a qualitative analysis of the justifications for these exclusion policies.ResultsSome TFFP signatories apply negative screening to other addictive industries (gambling: 35%; alcohol: 24%; cannabis 12% of signatories). There are important differences in the applied exclusion thresholds, with only 47% of TFFP signatories applying a zero-tolerance policy to tobacco. Thresholds are higher for other addictive industries. Signatories also differ in terms of their geographical and investor profiles. Justifications pertaining to compliance with international standards and reputational risks were the most common.ConclusionAddictive industries, such as tobacco, alcohol, gambling, and recreational cannabis, are increasingly excluded by investors. However, different understandings of RI influence how sector exclusions are implemented. Divesting from tobacco and other addictive industries is a crucial step toward a public health approach that prioritizes population health over financial profits. Prominent institutional investors are influential opinion leaders who can change the behavior of other investors and de-normalize controversial industries and reduce or prevent harm.
Research on the effectiveness and strategies of new media in promoting voluntary blood donation from a public health perspective in the post-pandemic era
Authors: Weng Jieqiu , Xu Yongzhu , Xie Chengbing , Tian Yunbo , Wang Fang , Cheng Ying 
Published: October 2024

ObjectiveThis study explores the effectiveness of new media in enhancing public enthusiasm for voluntary blood donation in the post-pandemic era and proposes effective publicity strategies to promote the sustainability and healthy development of blood donation activities.MethodsA questionnaire survey was widely used to collect public opinions and attitudes toward voluntary blood donation. The sample data underwent rigorous reliability and validity analysis to ensure authenticity and reliability. Statistical methods such as correlation analysis and regression analysis were employed to deeply investigate the underlying relationships between factors like new media publicity, emotional value, social recognition, convenience, information reliability, and willingness to donate blood voluntarily. Based on these analyses, a research model was constructed, and relevant hypotheses were verified through empirical methods.ResultsThe study found that new media publicity may be associated with increasing the willingness of the public to voluntarily donate blood. Survey participants indicated that they were more likely to consider donating blood after exposure to new media publicity. Among these factors, the emotional value and content conveyed by the publicity appeared to be particularly important. Additionally, our research revealed that social recognition, the convenience of blood donation, and the reliability of promotional information seemed to have a direct impact on the public’s willingness to donate blood. At the same time, these factors may indirectly promote actual blood donation behavior by enhancing the public’s emotional resonance and acceptance of blood donation.ConclusionThis study suggests that new media may play multiple positive roles in promoting voluntary blood donation. Based on these findings, we propose a series of strategic recommendations, including further optimizing publicity content, striving to enhance the public’s emotional resonance, improving the reliability of promotional information, and enhancing service convenience. These suggestions aim to potentially raise public awareness and willingness to participate in voluntary blood donation, providing a scientific basis and strong support for the promotion of voluntary blood donation in the post-epidemic era.
A single session of coordinative motor training does not improve spatial ability performances in healthy children
Authors: Morawietz Christina , Wissmann Anna Maria , Kuehne Till , Muehlbauer Thomas 
Published: October 2024

BackgroundIn recent years, studies have found small-to-medium positive effects of physical activity on academic achievement. Already acute bouts of exercise appear to improve certain cognitive functions. Spatial abilities are one aspect of cognition that is encountered frequently in daily life and that is closely related to success in science, technology, engineering, and mathematics (STEM)-subjects. However, little is known about the effects of an acute exercise session on spatial abilities. The aim of this study was therefore to evaluate the effect of a single session of coordinative motor training (CMT) on spatial ability performances in healthy children.MethodsForty-nine children were assigned to either a single session of CMT (i.e., obstacle course with motor coordinative and spatial elements) (n = 25, 12 females, mean age: 10.7 ± 0.6 years) or a resting control group (n = 24, 12 females, mean age ± SD: 11.4 ± 0.5 years). Spatial abilities were evaluated in both groups using the Paper Folding Test (PFT), Mental Rotation Test (MRT), Water Level Task (WLT), Corsi Block Test (CBT), and Numbered Cones Run (NCR).ResultsA statistical main effect for Test was observed for the majority of outcomes (i.e., all but the MRT). Test × Group interactions did not reach the level of significance.ConclusionThe results indicate that a single session of CMT does not improve spatial ability performances of healthy children. Future research should evaluate whether repeated longer-term interventions might be more suitable to generate significant improvements in spatial abilities.
Analysis of fungal diversity in the feces of Arborophila rufipectus
Authors: Ma Xiaoping , Li Junshu , Li Zhiguo , Chen Benping , Ling Zhenwen , Feng Shenglin , Zhong Zhijun , Peng Guangneng , Wang Ya , Jiang Yaozhang , Gu Yu 
Published: October 2024

BackgroundIntestinal fungal composition plays a crucial role in modulating host health, and thus is of great significance in the conservation of endangered bird species. However, research on gut fungal composition in birds is limited. Therefore, in this study, we aimed to examine gut fungal community and potential fecal pathogen composition in wild Arborophila rufipectus.MethodsFecal samples were collected from the habitats of wild A. rufipectus and Lophura nycthemera (a widely distributed species belonging to the same family as A. rufipectus) in summer and autumn. Thereafter, RNA was collected and the internal transcribed spacer rRNA gene was sequenced via high-throughput sequencing to investigate seasonal variations in intestinal core fungi, microbial fungi, and potential pathogenic fungi.ResultsThe gut microbiota of A. rufipectus and L. nycthemera were highly similar and mainly consisted of three phyla, Ascomycota (58.46%), Basidiomycota (28.80%), and Zygomycota (3.56%), which accounted for 90.82% of the fungal community in all the samples. Further, the predominant genera were Ascomycota_unclassified (12.24%), Fungi_unclassified (8.37%), Davidiella (5.18%), Helotiales_unclassified (2.76%), Wickerhamomyces (1.84%), and Pleosporales_unclassified (1.14%), and the potential fecal pathogens identified included Candida, Cryptococcus, Trichosporon, and Malassezia.ConclusionOur results provide evidence that the diversity of intestinal fungi in the endangered species, A. rufipectus, is similar to that in the common species, L. nycthemera, and may serve as a basis for monitoring the status of A. rufipectus and for developing conservation measures.
Photothermal Conversion Performance of Fe3O4/ATO Hybrid Nanofluid for Direct Absorption Solar Collector
Authors: Ham Jeonggyun , Kim Hyemin , Cho Honghyun 
Published: October 2024

In order to enhance the efficiency of direct absorption solar collectors, this study carried out an experimental analysis about the optical and photothermal conversion performance of Fe3O4, ATO (Antimony-doped tin oxide), and Fe3O4/ATO nanofluids with a total concentration of 0.1 wt%. According to the results of the experiments, Fe3O4 nanofluid outperforms ATO nanofluid in terms of optical absorption; nevertheless, at wavelengths shorter than 600 nm, it also shows significant scattering reflection. The solar-weighted absorption coefficient of Fe3O4/ATO nanofluid rose from 0.863 (mFe3O4/mTotal = 0.2) to 0.932 (mFe3O4/mTotal = 0.8) when the optical path length increased from 0.01 m to 0.06 m. Moreover, the Fe3O4/ATO hybrid nanofluid achieved a photothermal conversion efficiency of 0.932 when the mass ratio of Fe3O4 to total mass was 0.2, surpassing the efficiencies of 0.892 and 0.898 recorded for 0.1 wt% ATO and Fe3O4 nanofluids, respectively. When present together, the opposing optical characteristics of Fe3O4 and ATO boost photothermal conversion performance, which is anticipated to raise the efficiency of direct absorption solar collectors.
Bacillus clausii: A Review into Story of Its Probiotic Success and Potential Food Applications
Authors: Sadrimovahed Mahtab , Ulusoy Beyza H. 
Published: October 2024

Probiotics are live microbiota that can confer their hereditary health benefits upon the host. They can positively alter the diversity of the host’s gut microbiota population. Bacillus clausii is a spore-producing potential probiotic. Its application in the food industry has been highly recommended. Clausii spores are greatly resistant to harsh food processing treatment (heat and industrial pressure) and they can maintain their physiological traits (acid, bile salt) inside the human gut. The utilization of spores can enhance the nutritional viability of livestock, as well as the functionality of on-shelf products. This contemporary review covers the great attributes of B. clausii, including physiological characteristics, modes of action, probiotics benefits, a safety assessment, and the bacteria food industry applications for the purposes of producing enhanced functional foods known as probiotic foods. To our knowledge, although B. clausii has been widely applied in food industry, the amount of the literature that is dedicated to its role in sustainable food production and addresses its potential sporogenous probiotic behavior is fewer as compared to non-sporogenous lactic acid bacteria (Lactobacillus spp. and Bifidobacterium spp.). In our review, we also discovered a lack of empirical evidence on the synbiotic and synergistic behavior of clausii in combination with other active nutrients. Therefore, our review suggests that more studies should be conducted on the potential probiotic contribution of B. clausii in sustainable food production and its synergistic and synbiotic behavior in conjunction with other active nutrients.