bluuebunny/crossref_metadata_2025_split huggingface dataset:
A transcriptome analysis focusing on inflammation-related genes of grass carp intestines following infection with Aeromonas hydrophila
Authors: Song Xuehong , Hu Xiaolong , Sun Bingyao , Bo Yunxuan , Wu Kang , Xiao Lanying , Gong Chengliang 
Published: January 2017

AbstractInflammation is a protective response that is implicated in bacterial enteritis and other fish diseases. The inflammatory mechanisms behindAeromonas hydrophilainfections in fish remain poorly understood. In this study, we performed ade novograss carp transcriptome assembly using Illumina’s Solexa sequencing technique. On this basis we carried out a comparative analysis of intestinal transcriptomes fromA. hydrophila-challenged and physiological saline solution (PSS/mock) -challenged fish, and 315 genes were up-regulated and 234 were down-regulated in the intestines infected withA. hydrophila. The GO enrichment analysis indicated that the differentially expressed genes were enriched to 12, 4, and 8 GO terms in biological process, molecular function, and cellular component, respectively. A KEGG analysis showed that 549 DEGs were involved in 165 pathways. Moreover, 15 DEGs were selected for quantitative real-time PCR analysis to validate the RNA-seq data. The results confirmed the consistency of the expression levels between RNA-seq and qPCR data. In addition, a time-course analysis of the mRNA expression of 12 inflammatory genes further demonstrated that the intestinal inflammatory responses toA. hydrophilainfection simultaneously modulated gene expression variations. The present study provides intestine-specific transcriptome data, allowing us to unravel the mechanisms of intestinal inflammation triggered by bacterial pathogens.
The usefulness of CorvisST Tonometry and the Ocular Response Analyzer to assess the progression of glaucoma
Authors: Matsuura Masato , Hirasawa Kazunori , Murata Hiroshi , Nakakura Shunsuke , Kiuchi Yoshiaki , Asaoka Ryo 
Published: January 2017

AbstractCorneal Visualization Scheimpflug Technology (CST) and Ocular Response Analyzer (ORA) measurements were carried out in 105 eyes of 69 patients with primary open-angle glaucoma. All patients had axial length (AL), central corneal thickness (CCT), intraocular pressure (IOP) with Goldmann applanation tonometry (GAT) and eight visual fields (VF)s with the Humphrey Field Analyzer. VF progression was summarized using a time trend analysis of mean total deviation (mTD) and the association between mTD progression rate and a number of ocular parameters (including CST and ORA measurements) was assessed using mixed linear regression analysis. The optimal model of VF progression selected based on the corrected Akaike Information Criteria (AICc) included ORA’s corneal hysteresis (CH) parameter as well as a number of CST measurements: mTD progression rate = 1.2–0.070 * mean GAT + 0.090 * CH–1.5 * highest concavity deformation amplitude with CST + 9.4 * A1 deformation amplitude with CST–0.05 * A2 length with CST (AICc = 125.8). Eyes with corneas that experience deep indentation at the maximum deformation, shallow indentation at the first applanation and wide indentation at the second applanation in the CST measurement are more likely to experience faster rates of VF progression.
Somatostatin receptor expression on von Hippel-Lindau-associated hemangioblastomas offers novel therapeutic target
Authors: Sizdahkhani Saman , Feldman Michael J. , Piazza Martin G. , Ksendzovsky Alexander , Edwards Nancy A. , Ray-Chaudhury Abhik , Maric Dragan , Merrill Marsha J. , Pacak Karel , Zhuang Zhengping , Chittiboina Prashant 
Published: January 2017

AbstractVon Hippel-Lindau (VHL)-associated hemangioblastomas (VHL-HB) arise in the central nervous system (CNS), and are a leading cause of morbidity and mortality in VHL disease. Currently, surgical resection is the most effective way to manage symptomatic VHL-HBs. Surgically unresectable VHL-HBs or those in frail patients are challenging problems. Therapies targeting oncologic and vascular endothelial growth factor (VEGF) pathways have failed to demonstrate tumor control. Our experience and previous reports on VHL-HB avidity to somatostatin analogues suggested somatostatin receptor (SSTR) expression in VHL-HBs, offering an alternative therapeutic strategy. We explored this possibility by demonstrating consistent histologic expression of SSTR1, 2a, 4, and 5 in VHL-HBs. We found that somatostatin analogue octreotide induces apoptosis in VHL-HB stromal cells in a dose-dependent fashion by BAX – caspase-3 pathway unrelated to canonical VHL pathway. When administered to a patient with unresectable symptomatic suprasellar hemangioblastoma, octreotide resulted in tumor volume reduction, symptom stabilization, and tumor cytopenia on repeat 68Ga-DOTA-TATE positron emission tomography (PET) within 6 months, suggesting tumor infarction. We conclude that VHL-HBs harbor multiple SSTR subtypes that offer actionable chemo-therapeutic strategy for management of symptomatic, unresectable tumors by somatostatin analogue therapy.
Significantly enhanced creep resistance of low volume fraction in-situ TiBw/Ti6Al4V composites by architectured network reinforcements
Authors: Wang S. , Huang L. J. , Geng L. , Scarpa F. , Jiao Y. , Peng H. X. 
Published: January 2017

AbstractWe present a new class of TiBw/Ti6Al4V composites with a network reinforcement architecture that exhibits a significant creep resistance compared to monolithic Ti6Al4V alloys. Creep tests performed at temperatures between 773 K and 923 K and stress range of 100 MPa-300 MPa indicate both a significant improvement of the composites creep resistance due to the network architecture made by the TiB whiskers (TiBw), and a decrease of the steady-state creep rates by augmenting the local volume fractions of TiBw in the network region. The deformation behavior is driven by a diffusion-controlled dislocation climb process. Moreover, the activation energies of these composites are significantly higher than that of Ti6Al4V alloys, indicating a higher creep resistance. The increase of the activation energy can be attributed to the TiBw architecture that severely impedes the movements of dislocation and grain boundary sliding and provides a tailoring of the stress transfer. These micromechanical mechanisms lead to a remarkable improvement of the creep resistance of these networked TiBw/Ti6Al4V composites featuring the special networked architecture.
DRD2 co-expression network and a related polygenic index predict imaging, behavioral and clinical phenotypes linked to schizophrenia
Authors: Pergola G , Di Carlo P , D'Ambrosio E , Gelao B , Fazio L , Papalino M , Monda A , Scozia G , Pietrangelo B , Attrotto M , Apud J A , Chen Q , Mattay V S , Rampino A , Caforio G , Weinberger D R , Blasi G , Bertolino A 
Published: January 2017

AbstractGenetic risk for schizophrenia (SCZ) is determined by many genetic loci whose compound biological effects are difficult to determine. We hypothesized that co-expression pathways of SCZ risk genes are associated with system-level brain function and clinical phenotypes of SCZ. We examined genetic variants related to the dopamine D2 receptor gene DRD2 co-expression pathway and associated them with working memory (WM) behavior, the related brain activity and treatment response. Using two independent post-mortem prefrontal messenger RNA (mRNA) data sets (total N=249), we identified a DRD2 co-expression pathway enriched for SCZ risk genes. Next, we identified non-coding single-nucleotide polymorphisms (SNPs) associated with co-expression of this pathway. These SNPs were associated with regulatory genetic loci in the dorsolateral prefrontal cortex (P<0.05). We summarized their compound effect on co-expression into a Polygenic Co-expression Index (PCI), which predicted DRD2 pathway co-expression in both mRNA data sets (all P<0.05). We associated the PCI with brain activity during WM performance in two independent samples of healthy individuals (total N=368) and 29 patients with SCZ who performed the n-back task. Greater predicted DRD2 pathway prefrontal co-expression was associated with greater prefrontal activity and longer WM reaction times (all corrected P<0.05), thus indicating inefficient WM processing. Blind prediction of treatment response to antipsychotics in two independent samples of patients with SCZ suggested better clinical course of patientswith greater PCI (total N=87; P<0.05). The findings on this DRD2 co-expression pathway are a proof of concept that gene co-expression can parse SCZ risk genes into biological pathways associated with intermediate phenotypes as well as with clinically meaningful information.
Interactions between microsatellite instability and human gut colonization byEscherichia coliin colorectal cancer
Authors: Gagnière Johan , Bonnin Virginie , Jarrousse Anne-Sophie , Cardamone Emilie , Agus Allison , Uhrhammer Nancy , Sauvanet Pierre , Déchelotte Pierre , Barnich Nicolas , Bonnet Richard , Pezet Denis , Bonnet Mathilde 
Published: January 2017

Recent studies suggest that colonization of colonic mucosa by pathogenic Escherichia coli could be involved in the development of colorectal cancer (CRC), especially through the production of genotoxins such as colibactin and/or by interfering with the DNA mismatch repair (MMR) pathway that leads to microsatellite instability (MSI). The present study, performed on 88 CRC patients, revealed a significant increase in E. coli colonization in the MSI CRC phenotype. In the same way, E. coli persistence and internalization were increased in vitro in MMR-deficient cells. Moreover, we demonstrated that colibactin-producing E. coli induce inhibition of the mutL homologue 1 (MLH1) MMR proteins, which could lead to genomic instability. However, colibactin-producing E. coli were more frequently identified in microsatellite stable (MSS) CRC. The present study suggests differences in the involvement of colibactin-producing E. coli in colorectal carcinogenesis according to the CRC phenotype. Further host–pathogen interactions studies should take into account CRC phenotypes.
Qualitätsindikatoren für die Krankenhaushygiene in Internet-Patientenportalen – Analyse der Umsetzungshäufigkeit und Bewertung
der Eignung als Vergleichsparameter aus Krankenhaussicht
Authors: Haking Dennis 
Published: January 2017

Zusammenfassung
Zielsetzung Die in Internet-Patientenportalen genutzten Indikatoren für die Hygienequalität deutscher Krankenhäuser werden auf ihren
Umsetzungsgrad und ihre Eignung als Vergleichsparameter untersucht.
Methodik Mithilfe eines Onlinefragebogens wurde eine deutschlandweite Befragung gemäß der Zielsetzung durchgeführt. Die Datenanalyse
erfolgte mithilfe statistischer Analyseverfahren größen-, träger- und berufsgruppenspezifisch.
Ergebnisse Insbesondere rechtlich vorgeschriebene Maßnahmen wie Mitarbeiterschulungen und eine regelmäßig tagende Hygienekommission
werden von nahezu allen Krankenhäusern umgesetzt. Unterschiede gibt es bei den „freiwilligen“ Maßnahmen wie der „Aktion Saubere Hände“.
Diese werden speziell von den kleinen Häusern weniger häufig durchgeführt und auch in ihrer Eignung als Vergleichsparameter schlechter
bewertet. Auffällig ist, dass eine Vielzahl der Maßnahmen durch das Hygienepersonal deutlich besser bewertet wird als durch die
Geschäftsführung.
Schlussfolgerung Eine vollständige Transparenz ist für den Patienten nicht gegeben. Er kann nicht genau erkennen, welche Maßnahmen
gesetzlich vorgegeben und welche freiwillig sind, und kann dementsprechend die Bemühungen der einzelnen Krankenhäuser nicht richtig
einschätzen. Eine Vergleichbarkeit ist nur teilweise gegeben.
Antichlamydial Dimeric Indole Derivatives from Marine Actinomycete Rubrobacter radiotolerans
Authors: Li Jian , Chen Dandan , Huang Lei , Ni Min , Zhao Yu , Fan Huizhou , Bao Xiaofeng 
Published: January 2017

AbstractChlamydiae are widely distributed pathogens of human populations, which can lead to serious reproductive and other health problems. In our search for novel antichlamydial metabolites from marine derived-microorganisms, one new (1) and two known (2, 3) dimeric indole derivatives were isolated from the sponge-derived actinomycete Rubrobacter radiotolerans. The chemical structures of these metabolites were elucidated by NMR spectroscopic data as well as CD calculations. All three metabolites suppressed chlamydial growth in a concentration-dependent manner. Among them, compound 1 exhibited the most effective antichlamydial activity with IC50 values of 46.6 ~ 96.4 µM in the production of infectious progeny. Compounds appeared to target the mid-stage of the chlamydial developmental cycle by interfering with reticular body replication, but not directly inactivating the infectious elementary body.
Quantitative Determination of Lactones in Piper methysticum (Kava-Kava) by Supercritical Fluid Chromatography
Authors: Murauer Adele , Ganzera Markus 
Published: January 2017

AbstractA fast and validated supercritical fluid chromatography method for the quantitative determination of major lactones in Piper methysticum, a plant used against nervous anxiety, stress, and restlessness, was developed. The baseline separation of dihydrokavain, demethoxyyangonin, kavain, yangonin, dihydromethysticin, and methysticin was possible in less than 4 min on an Aquity UPC2 BEH 1.7 µm column, in combination with a mobile phase comprising CO2 and methanol with diethylamine. The column temperature had a great impact on the results because only at 70 °C could kavain and yangonin be fully resolved. With correlation coefficients above 0.998, recovery rates between 95.9 and 104.1 % as well as limit of detection values below 1.5 ng on-column, the procedure fulfilled all validation requirements and was well suited for the quantitative analysis of commercial products containing P. methysticum root powder and/or extract. All of them contained the target analytes, however, the absolute content of lactones was quite variable. Accordingly, depending on the product, the total daily intake of lactones varied from 56 to 312 mg. Concerning speed, selectivity, and environmental friendly operation, this supercritical fluid chromatography approach surpasses all previously reported ones.
What’s happening to our universities?
Authors: Martin Ben R. 
Published: January 2017

Abstract
In recent decades, many universities have been moving in the direction of a more hierarchical and centralised structure, with top-down planning and reduced local autonomy for departments. Yet, the management literature over this period has stressed the numerous benefits of flatter organisational structures, decentralisation and local autonomy for sections or departments. What might explain this paradox? And why have academics remained strangely quiet about this, meekly accepting their fate? This proposition paper critically examines the dangers of centralised top-down management, increasingly bureaucratic procedures, teaching to a prescribed formula, and research driven by assessment and performance targets, illustrating these with a number of specific examples. It discusses a number of possible forces driving these worrying developments, and concludes by asking whether academics may be in danger of suffering the fate of the boiled frog.
A chronicle of PA journals
Authors: Hooker Roderick S. 
Published: January 2017

ABSTRACT
At its semicentennial mark, the PA profession appears to be flourishing. Over the past 50 years, a number of journals have emerged to highlight the development of the profession and offer observations on it. The Physician Associate Journal, launched in 1970, was the first foray into PA reporting. Following this small effort, a dozen journals or newspapers came into print, lasted for a while, and then were replaced or died. Of journals that survived to the present, four are in English and one in Dutch. Three of the five journals are association-based, and four produce clinical articles; publication is monthly, quarterly, or semiannually. Two journals produce original health services research or studies on the PA education process and have citable track records. Readership of all journals is growing, and collectively these periodicals produce a body of scholarly work that clarifies the PA as a healthcare professional growing on a world stage. This article describes the journalistic history of one aspect of PA development.
Effect of Freeze-Dried Allograft Bone With Human Basic Fibroblast Growth Factor Containing a Collagen-Binding Domain From Clostridium histolyticum Collagenase on Bone Formation After Lumbar Posterolateral Fusion Surgery in Rats
Authors: Inoue Gen , Uchida Kentaro , Matsushita Osamu , Fujimaki Hisako , Saito Wataru , Miyagi Masayuki , Sekiguchi Hiroyuki , Nishi Nozomu , Ohtori Seiji , Yogoro Mizuki , Takaso Masashi 
Published: January 2017

Study Design.
An experimental study.

Objective.
To evaluate the effectiveness of freeze-dried bone allograft (FDBA) with basic fibroblast growth factor (bFGF) fused with the polycystic kidney disease domain (PKD) and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase, for the acceleration of lumbar posterolateral fusion in rats.

Summary of Background Data.
Reports indicate bFGF is an effective growth factor with osteogenic potential for promoting bone regeneration, although its efficiency decreases rapidly following its diffusion in body fluid from the host site. We developed a bFGF fusion protein containing the PKD and the CBD of C histolyticum collagenase (bFGF-PKD-CBD), which markedly enhanced bone formation at a relatively low concentration when applied to the surface of rat femurs in a previous study. The potential of this novel protein to accelerate bone fusion in a rat model of lumbar posterolateral fusion has yet to be investigated.

Methods.
Bilateral L4-L5 posterolateral fusions were performed, using 150 mg of FDBA powder per side. A total of 20 male Sprague-Dawley rats weighing 200 to 250 g/each were divided into two groups of 10 rats: FDBA was incubated with either phosphate-buffered saline (control group) or 0.58 nmol bFGF-PKD-CBD (bFGF-PKD-CBD group) before fusion surgery. The effect of bFGF-PKD-CBD was estimated using radiographs, microcomputed tomography, and histology (hematoxylin-eosin and von Kossa staining).

Results.
Both grafted bone volume in the posterolateral lesion and the volume of new bone formation on the surface of laminae and spinal processes were significantly higher in the bFGF-PKD-CBD group than in the control group. Histologically, new bone formation and surrounding chondrocytes and fibroblasts were prominent in the bFGF-PKD-CBD group.

Conclusion.
FDBA infused with bFGF-PKD-CBD may be a promising material for accelerating spinal fusion, and the FDBA-based delivery system for localizing bFGF-PKD-CBD may offer novel therapeutic approaches to augment spinal fusion.

Level of Evidence: N/A
Treatment of Minimally Invasive Hallux Valgus Surgery Complications
Authors: Yañez Arauz Juan M. 
Published: January 2017

Percutaneous foot surgery is a surgical method of treating various bone and soft-tissue disorders of the foot. In hallux valgus pathology, it is called minimal incision surgery, and is another method to treat this disorder. As in all surgical techniques, there are complications in minimal incision hallux valgus surgery. In general, in percutaneous surgery, there have been reports of recurrence on the hallux valgus deformity or development of the opposite deformity (hallux varus), malunion, nonunion, head metatarsal necrosis, clawed hallux, and transfer keratotic lesions that cause pain, thermal injuries, and other complications. We describe some of the most frequent complications in minimally invasive surgery of the hallux valgus, and how to correct it.Level of Evidence:Diagnostic Level 5. See Instructions for Authors for a complete description of levels of evidence.
Lesser Metatarsal Complications After MIS Surgery
Authors: Khazen Gabriel , Wagner Pablo , Wagner Emilio 
Published: January 2017

Minimally invasive surgery (MIS) for lesser metatarsals is performed through percutaneous incisions, with minimal scar formation. Nevertheless, MIS can have significant complications that may result in poor outcomes, forefoot pain, and metatarsalgia requiring further complex open revision surgery. These complications occur after inappropriate or poor surgical technique, lack of surgeon’s experience, and poor patient selection. The most frequent complication is metatarsalgia that occurs secondary to metatarsal malunion or nonunion. MIS is a technique that has to be carefully chosen for specific morbidities and performed only under experienced hands.
Level of Evidence: Diagnostic Level 5. See Instructions for Authors for a complete description of levels of evidence.
Racial and Ethnic Variation in Pediatric Cardiac Extracorporeal Life Support Survival
Authors: Chan Titus , Di Gennaro Jane , Farris Reid W. D. , Radman Monique , McMullan David Michael 
Published: January 2017

Objectives:
Previous studies have suggested an association between nonwhite race and poor outcomes in small subsets of cardiac surgery patients who require extracorporeal life support. This study aims to examine the association of race/ethnicity with mortality in pediatric patients who receive extracorporeal life support for cardiac support.

Design:
Retrospective analysis of registry data.

Setting:
Prospectively collected multi-institutional registry data.

Subjects:
Data from all North American pediatric patients in the Extracorporeal Life Support International Registry who received extracorporeal life support for cardiac support between 1998 and 2012 were analyzed. Multivariate regression models were constructed to examine the association between race/ethnicity and hospital mortality, adjusting for demographics, diagnosis, pre-extracorporeal life support care, extracorporeal life support variables, and extracorporeal life support-related complications.

Interventions:
None.

Measurements and Main Results:
Of 7,106 patients undergoing cardiac extracorporeal life support, the majority of patients were of white race (56.9%) with black race (16.7%), Hispanic ethnicity (15.8%), and Asian race (2.8%) comprising the other major race/ethnic groups. The mortality rate was 53.9% (n = 3,831). After adjusting for covariates, multivariate analysis identified black race (relative risk = 1.10; 95% CI, 1.04–1.16) and Hispanic ethnicity (relative risk = 1.08; 95% CI, 1.02–1.14) as independent risk factors for mortality.

Conclusions:
Black race and Hispanic ethnicity are independently associated with mortality in children who require cardiac extracorporeal life support.