bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial Spores: Cytocompatible Coating of Living Cells with Plant‐Derived Pyrogallol
Authors: Kim Ji Yup , Lee Hojae , Park Taegyun , Park Joonhong , Kim Mi‐Hee , Cho Hyeoncheol , Youn Wongu , Kang Sung Min , Choi Insung S. 
Published: October 2016

AbstractCell nanoencapsulation, generating cell‐in‐shell structures (“artificial spores“), provides a chemical toolbox for controlling the cellular behaviors and functional characteristics of individual cells. Among the shell materials studied so far, naturally occurring polyphenolic compounds, including polydopamine and tannic acid, have intensively been employed in cell‐surface engineering, because their material‐independent coating property eliminates an extra priming step for inducing subsequent shell formation. Albeit successful in generating cell‐in‐shell structures, the coating of polyphenolic compounds generally requires alkaline conditions and/or high salt conditions, which are not compatible with certain cell types. In this work, we demonstrate that the nanocoating of individual cells with a plant‐derived phenolic compound, pyrogallol (1,2,3‐trihydroxybenzene), occurs at mildly alkaline pH of 7.8 in an isotonic buffer. Three different cell types (anucleate, microbial, and mammalian cells) are coated with pyrogallol without noticeable decrease in cell viability. The protocol developed in this work could be applied to other polyphenolic compounds, and, considering the many polyphenols identified as a coating material, provides an advanced chemical tool in cell‐surface engineering.
Azo‐Polymer Janus Particles Assembled by Solvent‐Induced Microphase Separation and Their Photoresponsive Behavior
Authors: Zhou Xinran , Chen Shouping , Liu Bingyang , Wang Xiaogong 
Published: October 2016

AbstractWe report the successful fabrication of photoresponsive Janus particles (JPs) composed of an epoxy‐based azo polymer and poly(methyl methacrylate) (PMMA). Two representative azo polymers, of which one polymer (BP‐AZ‐CN) has cyano groups as electron‐withdrawing substituents on the azobenzene moieties and the other polymer (BP‐AZ‐CA) has carboxyl groups as the electron‐withdrawing substituents, were adopted for the investigation. The nanoscaled JPs, with a narrow size distribution and different azo polymer/PMMA ratios, were fabricated through self‐assembly in solution and as dispersions. Upon irradiation with linearly polarized light (λ=488 nm), two types of photoresponsive behavior were observed for JPs in the solid state. For JPs composed of BP‐AZ‐CN and PMMA, the light irradiation caused the azo‐polymer component to be stretched along the light polarization direction. Conversely, for JPs composed of BP‐AZ‐CA and PMMA, the azo‐polymer component became separated from PMMA component under the same irradiation conditions. These observations are valuable for a deeper understanding of the nature of self‐assembly and photoinduced mass‐transport at the nanometer scale.
Three‐Arm, Biotin‐Tagged Carbazole–Dicyanovinyl–Chlorambucil Conjugate: Simultaneous Tumor Targeting, Sensing, and Photoresponsive Anticancer Drug Delivery
Authors: Venkatesh Yarra , Karthik S. , Rajesh Y. , Mandal Mahitosh , Jana Avijit , Singh N. D. Pradeep 
Published: October 2016

AbstractThe design, synthesis, and in vitro biological studies of a biotin–carbazole–dicyanovinyl–chlorambucil conjugate (Bio‐CBZ‐DCV‐CBL; 6) are reported. This conjugate (6) is a multifunctional single‐molecule appliance composed of a thiol‐sensor DCV functionality, a CBZ‐derived phototrigger as well as fluorescent reporter, and CBL as the anticancer drug, and Bio as the cancer‐targeting ligand. In conjugate 6, the DCV bond undergoes a thiol–ene click reaction at pH<7 with intracellular thiols, thereby shutting down internal charge transfer between the donor CBZ and acceptor DCV units, resulting in a change of the fluorescence color from green to blue, and thereby, sensing the tumor microenvironment. Subsequent photoirradiation results in release of the anticancer drug CBL in a controlled manner.
Hysteresis‐Based Design of Dynamic Reference Trajectories to Avoid Saturation in Controlled Wind Turbines
Authors: Tutivén Christian , Vidal Yolanda , Acho Leonardo , Rodellar José 
Published: October 2016

AbstractThe main objective of this paper is to design a dynamic reference trajectory based on hysteresis to avoid saturation in controlled wind turbines. Basically, the torque controller and pitch controller set‐points are hysteretically manipulated to avoid saturation and drive the system with smooth dynamic changes. Simulation results obtained from a 5MW wind turbine benchmark model show that our proposed strategy has a clear added value with respect to the baseline controller (a well‐known and accepted industrial wind turbine controller). Moreover, the proposed strategy has been tested in healthy conditions but also in the presence of a realistic fault where the baseline controller caused saturation to finally conduct to instability.
Berücksichtigung der Längsbewehrung beim Durchstanznachweis nach Eurocode 2 (DIN EN 1992‐1‐1/NA)
Authors: Kueres Dominik , Siburg Carsten , Hegger Josef , Fingerloos Frank 
Published: October 2016

AbstractIm Jahr 2012 wurde der Eurocode 2 in Deutschland bauaufsichtlich eingeführt. Im Vergleich zu DIN 1045‐1 wurde unter anderem die Durchstanzbemessung von Flachdecken und Fundamenten bzw. Bodenplatten überarbeitet. Insbesondere die Berücksichtigung der Längsbewehrung führte in der Praxis zu einigen offenen Fragen. In diesem Beitrag werden daher aktuelle Auslegungen des DIN‐Normenausschusses Bauwesen (NABau) zur Ermittlung und Verteilung der Mindestmomente bei Wandecken und Wandenden sowie zur anrechenbaren Breite und Verankerung der Längsbewehrung für die Ermittlung des Längsbewehrungsgrades in Flachdecken und Fundamenten bzw. Bodenplatten vorgestellt.
Ansatz zur Quantifizierung der Nachhaltigkeit von Beton auf der Baustoffebene
Authors: Haist Michael , Moffatt Jack S. , Breiner Raphael , Vogel Michael , Müller Harald S. 
Published: October 2016

AbstractDer Gewährleistung einer möglichst hohen Nachhaltigkeit kommt im Bauwesen aufgrund der hohen Investitionskosten und des Energieverbrauchs bei der Errichtung beispielsweise von Infrastrukturbauwerken und den hohen Anforderungen an die Lebensdauer derartiger Bauwerke eine besonders große gesellschaftliche Bedeutung zu. Nachhaltiges Bauen ist dabei durch den Grundsatz geprägt, dass das Verhältnis aus gesellschaftlichem Nutzen von Bauwerken und dem dafür erforder lichen Energieaufwand und Ressourcenverbrauch infolge ihrer Errichtung, Nutzung und Entsorgung maximiert wird. Insbesondere für Infrastrukturbauwerke ist dies nur möglich, wenn die eingesetzten Werkstoffe eine möglichst hohe Leistungsfähigkeit und Dauerhaftigkeit aufweisen und – dies ist entscheidend –, wenn diese Eigenschaften auch durch den Planer möglichst optimal genutzt werden. Vor diesem Hintergrund beschreibt der vorliegende Beitrag Möglichkeiten und Wege, wie die Nachhaltigkeit des Werkstoffs Beton – dessen Herstellung für einen signifikanten Anteil beispielsweise des weltweiten CO2 Ausstoßes verantwortlich ist – signifikant gesteigert werden kann. Am Beispiel sogenannter Ökobetone – Betone mit stark reduziertem Zementgehalt – wird aufgezeigt, wie die Nachhaltigkeit des Werkstoffs nicht nur durch dessen Umwelteinwirkungen, sondern auch durch dessen Leistungsfähigkeit und Dauerhaftigkeit beeinflusst wird. Hierzu wird ein neuer Kennwert, das sog. Beton‐Nachhaltigkeitspotenzial, eingeführt, mit dem das Potenzial für eine nachhaltige Betonnutzung quantifiziert werden kann. Die Anwendung des Kennwerts wird im Beitrag anhand eines Beispiels erläutert.
Korrelation von Betondruck‐ und Zugfestigkeit historischer Betone
Authors: Weber Michael , Thiele Catherina 
Published: October 2016

AbstractIn der in Deutschland für die Bemessung von Stahlbeton‐ und Spannbetontragwerken aktuell bauaufsichtlich eingeführten DIN EN 1992‐1‐1 [1] sind Angaben zur Korrelation zwischen Betondruck‐ und Zugfestigkeit enthalten. Diese Regeln beziehen sich jedoch ausschließlich auf im Neubaufall verwendete Betone nach DIN EN 206‐1 [2]. Aufgrund verschiedener last‐ und zeitabhängiger Einflüsse kann die Gültigkeit der in DIN EN 1992‐1‐1 [1] aufgeführten Beziehung zwischen Betondruck‐ und Zugfestigkeit für Bestandsbetone nicht ohne weitere Überlegungen vorausgesetzt werden.Die Beeinflussung der Betondruck‐ und Zugfestigkeit durch z. B. Nacherhärtung und Karbonatisierung ist bisher unzureichend und noch nicht abschließend erforscht. Die in DIN EN 1992‐1‐1 [1] definierte Betonzugfestigkeit bezieht sich auf Untersuchungen an dauerhaft, d. h. bis zur Prüfung im Alter von 28 Tagen feucht gelagerten Probekörpern. Hinzu kommt, dass die tatsächlich am Bauwerk vorliegende Betonzugfestigkeit durch Mikrorissbildung infolge thermisch‐hygrischer Eigen spannungen wesentlich von der an Laborprobekörpern bestimmten Betonzugfestigkeit abweichen kann. Darüber hinaus ist der Einfluss der Mikrorissbildung auf die Betonzugfestigkeit größer als auf die Betondruckfestigkeit. Die tatsächlich am Bauwerk vorliegende Betonzugfestigkeit hat u. a. Einfluss auf die Querkrafttragfähigkeit sowie auf die Betonausbruchlast bei der Dimensionierung von Befestigungsmitteln.Zur Bewertung der Anwendbarkeit der Korrelation zwischen Betondruck‐ und Zugfestigkeit nach DIN EN 1992‐1‐1 [1] für historische Betone wurde an der TU Kaiserslautern ein von der Forschungsinitiative „Zukunft Bau” des Bundesamtes für Bauwesen und Raumordnung (BBR) gefördertes Forschungsprojekt (SWD‐10.08.18.7‐13.01) durchgeführt, dessen Ergebnisse nachfolgend dargestellt sind.
Fuß‐ und Radwegbrücke aus Carbonbeton in Albstadt‐Ebingen
Authors: Helbig Thorsten , Unterer Kay , Kulas Christian , Rempel Sergej , Hegger Josef 
Published: October 2016

AbstractDie im Oktober 2015 in Albstadt‐Ebingen realisierte Konstruktion verzichtet vollständig auf eine schlaffe oder vorgespannte Stahlbewehrung und ist damit die erste ausschließlich mit Textilfasern bewehrte Betonbrücke weltweit. Der Trogquerschnitt mit Materialstärken von 70 mm (Trogwände) bzw. 90 mm (Gehwegplatte) ist als monolithisches Fertigteil her gestellt worden. Das Brückendeck, das keiner weiteren Geh belagsaufbauten bedarf, wiegt bei einer Spannweite von 15 m und einer Breite von 3 m lediglich 14 t (ca. 310 kg/m2) und somit knapp die Hälfte eines vergleichbaren konventionellen Stahl betonbrückendecks. Neben Material‐ und Gewichtsersparnis kann von einer überdurchschnittlich langen Lebensdauer bei minimalem Wartungsaufwand ausgegangen werden, da die häufig schadenverursachende Stahlkorrosion des Stahlbetonbaus komplett umgangen wird. Der Einsatz von carbon faserbewehrtem Beton (Carbonbeton) ist in Deutschland derzeit bauaufsichtlich noch nicht zugelassen, daher musste eine Zustimmung im Einzelfall (ZiE) erwirkt werden.
Cardiovascular risk prevention and all-cause mortality in primary care patients with an abdominal aortic aneurysm
Authors: Bahia S S , Vidal-Diez A , Seshasai S R K , Shpitser I , Brownrigg J R , Patterson B O , Ray K K , Holt P J , Thompson M M , Karthikesalingam A 
Published: October 2016

Abstract

Background
Perioperative mortality is low for patients undergoing abdominal aortic aneurysm (AAA) repair, but long-term survival remains poor. Although patients diagnosed with AAA have a significant burden of cardiovascular disease and associated risk factors, there is limited understanding of the contribution of cardiovascular risk management to long-term survival.

Methods
General practice records within The Health Improvement Network (THIN) were examined. Patients with a diagnosis of AAA and at least 1 year of registered medical history were identified from 2000 to 2012. Medical therapies for cardiovascular risk were classified as antiplatelet, statin or antihypertensive agents. Progression to death was investigated using the G-computation formula with time-dependent co-variables to account for differences in exposure to cardiovascular risk-modifying treatments and the confounding between exposure, co-morbidities and death.

Results
Some 12 485 patients had a recorded diagnosis of AAA. From 2000 to 2012, prescription of medications that modify cardiovascular risk increased: from 26·6 to 76·7 per cent for statins, from 56·5 to 73·9 per cent for antiplatelet agents and from 75·3 to 84·0 per cent for antihypertensive drugs. Adjusted Kaplan–Meier curves demonstrated a better 5-year survival rate in patients receiving statins (68·4 versus 42·2 per cent), antiplatelet agents (63·6 versus 39·7 per cent) or antihypertensive agents (61·5 versus 39·1 per cent), compared with rates in patients not receiving each therapy.

Conclusion
Appropriate risk factor modification could significantly reduce long-term mortality in patients with AAA. In the UK, up to 30 per cent of patients are not currently receiving these medications.
Bowel function and quality of life after local excision or total mesorectal excision following chemoradiotherapy for rectal cancer
Authors: Pucciarelli S , Giandomenico F , De Paoli A , Gavaruzzi T , Lotto L , Mantello G , Barba C , Zotti P , Flora S , Del Bianco P 
Published: October 2016

Abstract

Background
Local excision for rectal cancer is expected to offer a better functional outcome than conventional surgery. The aim of the present study was to compare quality of life and bowel function in patients with rectal cancer who underwent either local excision or conventional surgery after chemoradiotherapy.

Methods
This was a retrospective multicentre study. Patients who underwent local excision were compared with those who had mesorectal excision. Quality of life and bowel function were investigated using validated questionnaires (European Organization for Research and Treatment of Cancer (EORTC) QLQ-C30, EORTC QLQ-CR29 and Memorial Sloan-Kettering Cancer Center Bowel Function Instrument) at a median follow-up of 49 (range 13–95) months. Further analysis was undertaken of data from patients who underwent local excision alone compared with those requiring subsequent radical surgery. Statistical significance was set at P &lt; 0·010.

Results
The mean constipation score was significantly better in the local excision group than in the mesorectal excision group (3·8 (95 per cent c.i. 0·3 to 7·2) versus 19·8 (12·1 to 27·4); P &lt; 0·001). Compared with patients who underwent mesorectal excision, those who had local excision had less sensation of incomplete emptying (mean score 3·7 (3·4 to 4·0) versus 2·8 (2·5 to 3·1); P &lt; 0·001) and second bowel movements within 15 min (mean score 3·6 (3·3 to 3·9) versus 3·0 (2·7 to 3·3); P = 0·006). Patients who underwent local excision alone scored better than those who had mesorectal excision, particularly for bowel function, who, in turn, scored better than patients requiring subsequent radical surgery following local excision.

Conclusion
Patients who underwent local excision had a better quality of life and bowel function than those who underwent mesorectal excision.
The E3 ubiquitin ligase RNF135 regulates the tumorigenesis activity of tongue cancer SCC25 cells
Authors: Jin Jian , Zhao liya , Li Zubing 
Published: October 2016

AbstractSeveral E3 ubiquitin ligases have been confirmed that they are related to the tumorigenesis. This study aims to find the tongue cancer‐related E3 ubiquitin ligase. The E3 ubiquitin ligase library was screened. The effect of candidate molecule on tongue cancer was validated through cell viability, cell proliferation, colony formation, invasive assay in vitro, and the xenograft model in vivo. The E3 ubiquitin ligase RNF135 significantly promoted the expression of PTEN and TP53 in SCC25 cells. The overexpression of RNF135 inhibited the viability, proliferation, and invasion of SCC25 cells. Knockdown of RNF135 had the opposite effects. Furthermore, RNF135 regulates the tumorigenesis activity of SCC25 cells in vivo. Our results demonstrated that RNF135 had the potential to affect the development of the tongue cancer in vitro. The further in vivo study is helpful to fully understand the role of it.
PD‐1 expression on the surface of peripheral blood CD4+ T cell and its association with the prognosis of patients with diffuse large B‐cell lymphoma
Authors: Zhang Wei , Bai Jie‐Fei , Zuo Meng‐Xuan , Cao Xin‐Xin , Chen Miao , Zhang Yan , Han Xiao , Zhong Ding‐Rong , Zhou Dao‐Bin 
Published: October 2016

AbstractThe aim of the study was to investigate the relationship between PD‐1 expression on the surface of CD4+ T cells and prognosis of patients with diffuse large B‐cell lymphoma (DLBCL). Sixty patients who were newly diagnosed with DLBCL and 39 healthy controls were enrolled. In CD4+ T cells of DLBCL patients, the median MFI of PD‐1 were 541.5 (range: 348.25–758.75), significantly higher than 250 (range: 211–326) in healthy controls (P < 0.001). The ZAP70, PI3K, and NFAT mRNA expression levels of patients were 0.47, 0.47, and 0.62 times, respectively, of those of the healthy controls (P < 0.05). In patients with the percentage of PD‐1 on CD4+ T cells ≥30.25%, their EFS and OS were significantly lower than patients with PD‐1+ CD4+ T cells <30.25% (P < 0.05). The possible explanation is that high PD‐1 expression on CD4+ cells of DLBCL patients may impair T‐cell function and thus contribute to poor prognosis. There was no relationship between PD‐1 surface expression on CD4+ T cells and PD‐1 expression within the biopsy of tumor microenvironments from DLBCL patients.
Quercetin suppresses lung cancer growth by targeting Aurora B kinase
Authors: Xingyu Zhu , Peijie Ma , Dan Peng , Youg Wang , Daojun Wang , Xinzheng Chen , Xijun Zhang , Yangrong Song 
Published: October 2016

Abstractaurora B kinase is highly expressed in several cancer cells and promotes tumorigenesis and progression, and therefore, it is an important target for drug to treat tumors. Quercetin was identified to be an antitumor agent. Herein, we report for the first time that quercetin inhibited aurora B activities by directly binding with aurora B in vitro and in vivo. Ex vivo studies showed that quercetin inhibited aurora B activities in JB6 Cl41 cells and A549 lung cancer cells. Moreover, knockdown of aurora B in A549 cells decreased their sensitivities to quercetin. In vivo study demonstrated that injection of quercetin in A549 tumor‐bearing mice effectively suppressed cancer growth. The phosphorylation of histone 3 in tumor tissues was also decreased after quercetin treatment. In short, quercetin can suppress growth of lung cancer cells as an aurora B inhibitor both in vitro and in vivo.
Constructions of emotional impact, risk and meaning among practitioners working with asylum seekers and refugees
Authors: Apostolidou Zoe 
Published: October 2016

AbstractAimThis study examines the emotional impact that clinical work with asylum seekers and refugees has on practitioners who work with them. This examination takes place by unpacking on the one hand, notions of risk and trauma whilst on the other hand, notions of meaning among practitioners.MethodThe study analyses the discourses deriving from eight semi‐structured interviews that were conducted with specialist practitioners who have worked with asylum seekers and refugees in a psychotherapeutic context.FindingsThe findings of the study reveal that clients’ traumatic experiences permeated practitioners’ discourses and had a profound impact on practitioners’ way of perceiving the world and themselves. Despite this impact, the findings also show that clinical work with asylum seekers and refugees is constructed as meaningful and rewarding for practitioners. Practitioners appear to locate their clinical work in a wider context of social engagement that envelops a notion of social responsibility towards vulnerable individuals and which allows them to feel that they can make a positive contribution to their community. Importantly, practitioners’ social engagement with clinical work appears to counterbalance the traumatising impact that work with asylum seekers and refugees has upon them and to produce a meaningful experience.
Glassworts as Possible Anticancer Agents Against Human Colorectal Adenocarcinoma Cells with Their Nutritive, Antioxidant and Phytochemical Profiles
Authors: Altay Ahmet , Celep Gulcin Sagdicoglu , Yaprak Ahmet E. , Baskose Isa , Bozoglu Faruk 
Published: October 2016

In this study, the possible uses of glassworts as potential food ingredients and their antiproliferative activity against colorectal adenocarcinoma cells together with their antioxidant and phytochemical profiles were investigated for the first time. MeOH extracts of five different taxa collected from different localities were screened for their antioxidant capacities by DPPH (IC50 2.91 – 5.49 mg/ml) and ABTS (24.4 – 38.5 μmol TE/g extract) assays. Salicornia freitagii exhibited the highest DPPH radical scavenging activity. LC/MS/MS analysis displayed that vanillic acid and p‐coumaric acid were two main phenolic compounds in the extract. Salicornia freitagii extracts also exhibited high antiproliferative activity against HT‐29 (IC50 1.67 mg/ml) and Caco‐2 (IC50 3.03 mg/ml) cells for 72 h. Mineral analysis indicated that all the species with different proportions of elemental components contained high amount of cations. These results indicate that investigated glassworts, with their high phenolic and mineral contents and also notable antioxidant and cytotoxic properties, may be utilized as a promising source of therapeutics.
From early dating violence to adult intimate partner violence: Continuity and sources of resilience in adulthood
Authors: Greenman Sarah J. , Matsuda Mauri 
Published: October 2016

AbstractBackgroundPrevious literature has found continuity for intimate partner violence, but little research has explored continuity between dating violence and adult intimate partner violence (IPV) or whether protective factors may attenuate this relationship.AimsThis research hypothesised a positive relationship between dating violence in early adulthood and later adulthood IPV and that support and attachment would provide buffering and direct protection for this relationship.MethodsData from the Rochester Youth Development Study were used to explore these questions through negative binomial regression.ResultsDating violence was statistically significantly related to an increase of adult IPV. Family support, parental reports of attachment to the subject, peer support and parenting‐related social support all were protective factors that provided a direct effect for those respondents perpetrating dating violence. None of the protective factors provided buffering protection between dating violence and adult IPV.ConclusionsResults confirm significant continuity between dating violence and IPV and that support from peers and family, parenting‐related support and parental reports of attachment protect an individual from continuing to engage in intimate partner violence throughout adulthood. Bolstering these supportive relationships may help provide points of intervention to interrupt the link between early dating violence and later adulthood IPV. Copyright © 2016 John Wiley & Sons, Ltd.