bluuebunny/crossref_metadata_2025_split huggingface dataset:
New insights into the electrochemical behavior of acid orange 7: Convergent paired electrochemical synthesis of new aminonaphthol derivatives
Authors: Momeni Shima , Nematollahi Davood 
Published: February 2017

AbstractElectrochemical behavior of acid orange 7 has been exhaustively studied in aqueous solutions with different pH values, using cyclic voltammetry and constant current coulometry. This study has provided new insights into the mechanistic details, pH dependence and intermediate structure of both electrochemical oxidation and reduction of acid orange 7. Surprisingly, the results indicate that a same redox couple (1-iminonaphthalen-2(1H)-one/1-aminonaphthalen-2-ol) is formed from both oxidation and reduction of acid orange 7. Also, an additional purpose of this work is electrochemical synthesis of three new derivatives of 1-amino-4-(phenylsulfonyl)naphthalen-2-ol (3a–3c) under constant current electrolysis via electrochemical oxidation (and reduction) of acid orange 7 in the presence of arylsulfinic acids as nucleophiles. The results indicate that the electrogenerated 1-iminonaphthalen-2(1 H)-one participates in Michael addition reaction with arylsulfinic acids to form the 1-amino-3-(phenylsulfonyl)naphthalen-2-ol derivatives. The synthesis was carried out in an undivided cell equipped with carbon rods as an anode and cathode.
Black TiO2 nanobelts/g-C3N4 nanosheets Laminated Heterojunctions with Efficient Visible-Light-Driven Photocatalytic Performance
Authors: Shen Liyan , Xing Zipeng , Zou Jinlong , Li Zhenzi , Wu Xiaoyan , Zhang Yuchi , Zhu Qi , Yang Shilin , Zhou Wei 
Published: February 2017

AbstractBlack TiO2 nanobelts/g-C3N4 nanosheets laminated heterojunctions (b-TiO2/g-C3N4) as visible-light-driven photocatalysts are fabricated through a simple hydrothermal-calcination process and an in-situ solid-state chemical reduction approach, followed by the mild thermal treatment (350 °C) in argon atmosphere. The prepared samples are evidently investigated by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, N2 adsorption, and UV-visible diffuse reflectance spectroscopy, respectively. The results show that special laminated heterojunctions are formed between black TiO2 nanobelts and g-C3N4 nanosheets, which favor the separation of photogenerated electron-hole pairs. Furthermore, the presence of Ti3+ and g-C3N4 greatly enhance the absorption of visible light. The resultant b-TiO2/g-C3N4 materials exhibit higher photocatalytic activity than that of g-C3N4, TiO2, b-TiO2 and TiO2/g-C3N4 for degradation of methyl orange (95%) and hydrogen evolution (555.8 μmol h−1g−1) under visible light irradiation. The apparent reaction rate constant (k) of b-TiO2/g-C3N4 is ~9 times higher than that of pristine TiO2. Therefore, the high-efficient laminated heterojunction composites will have potential applications in fields of environment and energy.
Transcriptome sequencing identifies novel persistent viruses in herbicide resistant wild-grasses
Authors: Sabbadin Federico , Glover Rachel , Stafford Rebecca , Rozado-Aguirre Zuriñe , Boonham Neil , Adams Ian , Mumford Rick , Edwards Robert 
Published: February 2017

AbstractHerbicide resistance in wild grasses is widespread in the UK, with non-target site resistance (NTSR) to multiple chemistries being particularly problematic in weed control. As a complex trait, NTSR is driven by complex evolutionary pressures and the growing awareness of the role of the phytobiome in plant abiotic stress tolerance, led us to sequence the transcriptomes of herbicide resistant and susceptible populations of black-grass and annual rye-grass for the presence of endophytes. Black-grass (Alopecurus myosuroides; Am) populations, displaying no overt disease symptoms, contained three previously undescribed viruses belonging to the Partititiviridae (AMPV1 and AMPV2) and Rhabdoviridae (AMVV1) families. These infections were widespread in UK black-grass populations and evidence was obtained for similar viruses being present in annual rye grass (Lolium rigidum), perennial rye-grass (Lolium perenne) and meadow fescue (Festuca pratensis). In black-grass, while no direct causative link was established linking viral infection to herbicide resistance, transcriptome sequencing showed a high incidence of infection in the NTSR Peldon population. The widespread infection of these weeds by little characterised and persistent viruses and their potential evolutionary role in enhancing plant stress tolerance mechanisms including NTSR warrants further investigation.
Fungal endophyte-derived Fritillaria unibracteata var. wabuensis: diversity, antioxidant capacities in vitro and relations to phenolic, flavonoid or saponin compounds
Authors: Pan Feng , Su Tian-Jiao , Cai Shi-Mei , Wu Wei 
Published: February 2017

AbstractDiverse fungal endophytes are rich fungal resources for the production of an enormous quantity of natural products. In the present study, 53 fungal endophytes were isolated from the bulbs of Fritillaria unibracteata var. wabuensis (FUW). Of these, 49 strains were identified and grouped into 17 different taxa, and priority was conferred to the Fusarium genus. All fungal fermented filtrates displayed antioxidant activities. The DPPH activity, total antioxidant capacities (ABTS), reduction power (FRAP), total phenolic content (TPC), total flavonoid content (TFC) and total saponin content (TSC) were evaluated using petroleum ether, ethyl acetate, n-butyl alcohol and ethanol fractions extracted from five representative fungal cultures. The last three fractions showed more potent antioxidant activity than the first fraction. Significant positive correlations were found between the compositions (TPC, TFC and TSC) and antioxidant capacities (DPPH, ABTS and FRAP). In addition, multifarious natural antioxidant components were identified from the fungal extracts, including gallic acid, rutin, phlorizin, 2,4-di-tert-butylphenol and 2,6-di-tert-butyl hydroquinone; these were determined preliminarily by TLC-bioautography, HPLC and GC-MS analysis. This study showed abundant fungal resources in FUW. Phenolics, flavonoids and saponins are crucial bioactive constituents in these abundant fungal endophytes and can be viewed as new potential antioxidant resources.
The ATP binding cassette transporter, ABCG1, localizes to cortical actin filaments
Authors: Pandzic Elvis , Gelissen Ingrid C. , Whan Renee , Barter Philip J. , Sviridov Dmitri , Gaus Katharina , Rye Kerry-Anne , Cochran Blake J. 
Published: February 2017

AbstractThe ATP-binding cassette sub-family G member 1 (ABCG1) exports cellular cholesterol to high-density lipoproteins (HDL). However, a number of recent studies have suggested ABCG1 is predominantly localised to intracellular membranes. In this study, we found that ABCG1 was organized into two distinct cellular pools: one at the plasma membrane and the other associated with the endoplasmic reticulum (ER). The plasma membrane fraction was organized into filamentous structures that were associated with cortical actin filaments. Inhibition of actin polymerization resulted in complete disruption of ABCG1 filaments. Cholesterol loading of the cells increased the formation of the filamentous ABCG1, the proximity of filamentous ABCG1 to actin filaments and the diffusion rate of membrane associated ABCG1. Our findings suggest that the actin cytoskeleton plays a critical role in the plasma membrane localization of ABCG1.
Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer
Authors: Song Yongxi , Wang Jun , Cheng Zhongyi , Gao Peng , Sun Jingxu , Chen Xiaowan , Chen Chen , Wang Yunlong , Wang Zhenning 
Published: February 2017

AbstractWith the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of cancers have been comprehensively explored. However, cancer is a multi-dimensional disease with sophisticated regulations, and few studies focus on the crosstalk among multiomics. In order to explore the molecular mechanisms of gastric cancer (GC), particularly in the process of lymph node metastasis (LNM), we investigated dynamic profiling changes as well as crosstalk between long non-coding RNAs (lncRNAs), the proteome, and the lysine succinylome. Our study reports the first qualitative and quantitative profile of lysine succinylation in GC. We identified a novel mechanism through which the TCA cycle and pentose phosphate pathway might be regulated through lysine succinylation in their core enzymes. We then examined the potential of using lysine succinylation as a biomarker for GC and successfully developed a succinylation-dependent antibody for the K569 site in Caldesmon as putative biomarker. Finally, we investigated the relationship between the lysine succinylome and lncRNAs, identifying potential crosstalks between two lncRNAs and one succinylation site. These results expand our understanding of the mechanisms of tumorigenesis and provide new information for the diagnosis and prognosis of GC.
Proteome and Acetylome Analysis Identifies Novel Pathways and Targets Regulated by Perifosine in Neuroblastoma
Authors: Gu Xiao , Hua Zhongyan , Dong Yudi , Zhan Yue , Zhang Xiaowen , Tian Wei , Liu Zhihui , Thiele Carol J. , Li Zhijie 
Published: February 2017

AbstractPerifosine, an Akt inhibitor, has been shown to be effective in controlling neuroblastoma tumor growth. However, studies indicate that in addition to the ability to inhibit Akt, other mechanisms contribute to perifosine’s anti-tumor activity. To gain insight into perifosine anti-tumor activity in neuroblastoma we have studied changes in the proteome and acetylome after perifosine treatment in SK-N-AS neuroblastoma cells using SILAC labeling, affinity enrichment, high-resolution and LC-MS/MS analysis. Bioinformatic analysis indicates that, a total of 5,880 proteins and 3,415 lysine acetylation sites were quantified in SK-N-AS cells and 216 differentially expressed proteins and 115 differentially expressed lysine acetylation sites were obtained. These differentially expressed proteins and lysine acetylated proteins were involved in a number of different biological functions, metabolic pathways and pathophysiological processes. This study details the impact of perifosine on proteome and lysine acetylome in SK-N-AS cells and expands our understanding of the mechanisms of perifosine action in neuroblastoma.
Turbulent chimeras in large semiconductor laser arrays
Authors: Shena J. , Hizanidis J. , Kovanis V. , Tsironis G. P. 
Published: February 2017

AbstractSemiconductor laser arrays have been investigated experimentally and theoretically from the viewpoint of temporal and spatial coherence for the past forty years. In this work, we are focusing on a rather novel complex collective behavior, namely chimera states, where synchronized clusters of emitters coexist with unsynchronized ones. For the first time, we find such states exist in large diode arrays based on quantum well gain media with nearest-neighbor interactions. The crucial parameters are the evanescent coupling strength and the relative optical frequency detuning between the emitters of the array. By employing a recently proposed figure of merit for classifying chimera states, we provide quantitative and qualitative evidence for the observed dynamics. The corresponding chimeras are identified as turbulent according to the irregular temporal behavior of the classification measure.
Terahertz imaging for early screening of diabetic foot syndrome: A proof of concept
Authors: Hernandez-Cardoso G. G. , Rojas-Landeros S. C. , Alfaro-Gomez M. , Hernandez-Serrano A. I. , Salas-Gutierrez I. , Lemus-Bedolla E. , Castillo-Guzman A. R. , Lopez-Lemus H. L. , Castro-Camus E. 
Published: February 2017

AbstractMost people with diabetes suffer some deterioration of the feet. Diabetic foot syndrome causes ulceration in about 15% of cases and such deterioration leads to amputation in about 2.5% of diabetic patients, diminishing their quality of life and generating extraordinary costs for patients and public health systems. Currently, there is no objective method for the detection of diabetic foot syndrome in its early stages. We propose terahertz imaging as a method for the evaluation of such deterioration. This screening method could aid the prevention and medical treatment of this condition in the future.
A novel indication of platonin, a therapeutic immunomodulating medicine, on neuroprotection against ischemic stroke in mice
Authors: Sheu Joen-Rong , Chen Zhih-Cherng , Jayakumar Thanasekaran , Chou Duen-Suey , Yen Ting-Lin , Lee Hsing-Ni , Pan Szu-Han , Hsia Chih-Hsuan , Yang Chih-Hao , Hsieh Cheng-Ying 
Published: February 2017

AbstractThrombosis and stroke are major causes of disability and death worldwide. However, the regular antithrombotic drugs may have unsatisfactory results and side effects. Platonin, a cyanine photosensitizing dye, has been used to treat trauma, ulcers and some acute inflammation. Here, we explored the neuroprotective effects of platonin against middle cerebral artery occlusion (MCAO)-induced ischemic stroke in mice. Platonin(200 μg/kg) substantially reduced cerebral infarct volume, brain edema, neuronal cell death and neurological deficit scores, and improved the MCAO-reduced locomotor activity and rotarod performance. Platonin(5–10 μM) potently inhibited platelet aggregation and c-Jun NH2-terminal kinase (JNK) phosphorylation in collagen-activated platelets. The antiaggregation effect did not affect bleeding time but increased occlusion time in platonin(100 and 200 μg/kg)-treated mice. Platonin(2–10 μM) was potent in diminishing collagen- and Fenton reaction-induced ∙OH formation. Platonin(5–10 μM) also suppressed the expression of nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, interleukin-1β, and JNK phosphorylation in lipopolysaccharide-stimulated macrophages. MCAO-induced expression of 3-nitrotyrosine and Iba1 was apparently attenuated in platonin(200 μg/kg)-treated mice. In conclusion, platonin exhibited remarkable neuroprotective properties against MCAO-induced ischemia in a mouse model through its antiaggregation, antiinflammatory and antiradical properties. The observed therapeutic efficacy of platonin may consider being a novel medcine against ischemic stroke.
Code-switching attitudes and their effects on acceptability judgment tasks
Authors: Badiola Lucia , Delgado Rodrigo , Sande Ariane , Stefanich Sara 
Published: February 2017

Abstract
The present study examines the effects of code-switching (CS) attitudes in Acceptability Judgment Tasks (AJTs) among early Spanish/English bilinguals in the United States. In doing so, we explore whether negative attitudes towards CS result in lower/degraded ratings, and, likewise, whether positive attitudes result in higher acceptability ratings. Fifty Spanish/English bilinguals completed a survey that comprised a linguistic background questionnaire and a set of monolingual and code-switched sentences featuring two sets of stimuli, pro-drop (Sande, 2015) and pronouns (Koronkiewicz, 2014), that they rated on a 1–7 Likert scale; additionally, the survey included a final component that gathered information about the speakers’ attitudes towards CS. The pro-drop and pronouns code-switched stimuli gave rise to a total of four conditions. Results from a Linear Mixed Model revealed that all participants, regardless of attitude, distinguished between all Conditions. Furthermore, an effect for attitude was found for two of the conditions, such that the more positive the attitude, the higher the rating given on the AJT. In fact, these two conditions were composed of the CS structures that were rated higher by participants in Sande (2015) and Koronkiewicz (2014). No effect for attitude was found for CS structures that were rated low in the original studies. Thus, this investigation suggests that the attitudes that bilingual speakers have towards CS play a role in the ratings that they provide in AJTs, but in a manner that highlights, rather than obscures, the rule-governed nature of CS.
Isolation and Characterization of Large Marine Bacteriophage (Myoviridae), VhKM4 Infecting Vibrio harveyi
Authors: Lal Tamrin M. , Sano Motohiko , Ransangan Julian 
Published: February 2017

Abstract
The causative agent responsible for vibriosis in tropical fish aquaculture, Vibrio harveyi, has become a major bacterial pathogen. Studies suggest that this bacterium has developed resistance to antibiotics commonly used in aquaculture. In view of this situation and the requirement for the proposed postantibiotic era, bacteriophage therapy seems to be a promising control strategy for fish vibriosis. In this study, a lytic Vibrio phage VhKM4 belonging to a member of large, marine Myoviridae was successfully isolated. It exhibited bacteriolysis to both V. harveyi VHJR7 and V. parahaemolyticus ATCC 17802. The latent period of the VhKM4 phage was recorded at 60 min. It also recorded average burst size of approximately 52 plaque-forming units per infected cell. A strong bacteriolytic activity at low multiplicity of infection of 0.01 indicates the effectiveness of this large marine myovirid against fish pathogenic strain of V. harveyi VHJR7.
Received June 16, 2016; accepted October 7, 2016 Published online February 6, 2017
Saprolegniosis in Nile Tilapia: Identification, Molecular Characterization, and Phylogenetic Analysis of Two Novel Pathogenic Saprolegnia Strains
Authors: Zahran Eman , Hafez Elsayed E. , Mohd Altaf Hossain Ferdaus , Elhadidy Mohamed , Shaheen Adel A. 
Published: February 2017

Abstract
Saprolegniosis is a fungal infection that leads to huge economic losses in tilapia aquaculture. Saprolegnia spp. are usually implicated as the etiological agents, but their identification is sometimes troublesome and confusing. In this study, two Saprolegnia strains (ManS22 and ManS33) were isolated from Nile Tilapia Oreochromis niloticus suffering from saprolegniosis. Both isolates were characterized morphologically and from internal transcribed spacer (ITS) sequence data. Additionally, both strains were tested for pathogenicity, and they were highly pathogenic and caused cumulative mortalities of 88.9% and 95.6%, respectively. Initially, the two strains were identified, by morphology of sexual and asexual stages, as members of the genus Saprolegnia. For more definitive identification and characterization, the ITS region of the ribosomal RNA genes was amplified and sequenced, and sequences were compared with other known sequences in GenBank. A phylogenetic tree constructed using the neighbor-joining method revealed that the two strains fell into two clusters within the species Saprolegnia parasitica. Cluster 1 included the ManS33 strain and cluster 2 the ManS22 strain. Cluster 1 grouped the ManS33 strain with other S. parasitica stains and shared 97–99% sequence similarity. Cluster 2 contained only the ManS22 strain and shared 93–94% similarity to several reference sequences of S. parasitica strains. Therefore, our findings suggest that ManS22 represents a newly described strain of S. parasitica.
Received April 19, 2016; accepted October 27, 2016 Published online February 6, 2017
Transcriptomic characterization of gall tissue of Japanese elm tree (Ulmus davidiana var. japonica) induced by the aphid Tetraneura nigriabdominalis
Authors: Takei Mami , Ito Shinsaku , Tanaka Keisuke , Ishige Taichiro , Suzuki Yoshihito 
Published: February 2017

Abstract
Insect galls are abnormal plant tissues induced by parasitic insect(s) for use as their habitat. In previous work, we suggested that gall tissues induced by the aphid Tetraneura nigriabdominalis on Japanese elm trees are less responsive than leaf tissues to jasmonic acid (JA), which is involved in the production of volatile organic compounds as a typical defensive reaction of plants against attack by insect pests. A comprehensive analysis of gene expression by RNA sequencing indicated that the number of JA responsive genes was markedly lower in gall tissues than in leaf tissues. This suggests that gall tissues are mostly defective in JA signaling, although JA signaling is not entirely compromised in gall tissue. Gene ontology analysis sheds light on some stress-related unigenes with higher expression levels in gall tissues, suggesting that host plants sense aphids as a biotic stress but are defective in the JA-mediated defense response in gall tissues.
Trends and Potential Drivers of Distribution Overlap of River Herring and Commercially Exploited Pelagic Marine Fishes on the Northeast U.S. Continental Shelf
Authors: Turner Sara M. , Hare Jonathan A. , Richardson David E. , Manderson John P. 
Published: February 2017

Abstract
Concern has been increasing over the impacts of incidental catches of river herring (Alewife Alosa pseudoharengus and Blueback Herring A. aestivalis) in commercial fisheries for Atlantic Herring Clupea harengus and Atlantic Mackerel Scomber scombrus, but historical information on mixed-species catches and their driving factors is lacking. We examined data from spring and fall bottom trawl surveys (National Oceanic and Atmospheric Administration, Northeast Fisheries Science Center) for temporal trends in overlap and potential ecosystem drivers of overlap trends. Species overlap changed substantially over time, with changes varying by season and region of the northeastern U.S. continental shelf. In the fall, changes in overlap were positively related to abundance in Georges Bank and southern New England. In the spring, most changes in species overlap were positively related to increasing species abundances. Positive relationships between overlap and abundance suggested that as species distributions increased with abundance, distributional overlap with other species increased as well. During the fall, changes in species overlap were positively related to bottom temperature in the Gulf of Maine, whereas in the spring, the relationship with bottom temperature was less consistent. Temperature-related changes in overlap implied that species distributions changed at different rates in response to climatic changes, thus leading to changes in overlap. Our findings suggest that fisheries management should take an adaptive approach to managing incidental catches by using region- and season-specific policies and should incorporate the effects of species abundances and climate change on species interactions.