bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
Authors: Zhu Xiaoyu , Liu Xin , Cheng Zhongyi , Zhu Jun , Xu Lei , Wang Fengsong , Qi Wulin , Yan Jiawei , Liu Ning , Sun Zimin , Liu Huilan , Peng Xiaojun , Hao Yingchan , Zheng Nan , Wu Quan 
Published: January 2016

AbstractValproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA) are both HDAC inhibitors (HDACi). Previous studies indicated that both inhibitors show therapeutic effects on acute myeloid leukaemia (AML), while the differential impacts of the two different HDACi on AML treatment still remains elusive. In this study, using 3-plex SILAC based quantitative proteomics technique, anti-acetyllysine antibody based affinity enrichment, high resolution LC-MS/MS and intensive bioinformatic analysis, the quantitative proteome and acetylome in SAHA and VPA treated AML HL60 cells were extensively studied. In total, 5,775 proteins and 1,124 lysine acetylation sites were successfully obtained in response to VAP and SAHA treatment. It is found that VPA and SAHA treatment differently induced proteome and acetylome profiling in AML HL60 cells. This study revealed the differential impacts of VPA and SAHA on proteome/acetylome in AML cells, deepening our understanding of HDAC inhibitor mediated AML therapeutics.
Growth control of oxygen stoichiometry in homoepitaxial SrTiO3 films by pulsed laser epitaxy in high vacuum
Authors: Lee Ho Nyung , Ambrose Seo Sung S. , Choi Woo Seok , Rouleau Christopher M. 
Published: January 2016

AbstractIn many transition metal oxides, oxygen stoichiometry is one of the most critical parameters that plays a key role in determining the structural, physical, optical and electrochemical properties of the material. However, controlling the growth to obtain high quality single crystal films having the right oxygen stoichiometry, especially in a high vacuum environment, has been viewed as a challenge. In this work, we show that, through proper control of the plume kinetic energy, stoichiometric crystalline films can be synthesized without generating oxygen defects even in high vacuum. We use a model homoepitaxial system of SrTiO3 (STO) thin films on single crystal STO substrates. Physical property measurements indicate that oxygen vacancy generation in high vacuum is strongly influenced by the energetics of the laser plume and it can be controlled by proper laser beam delivery. Therefore, our finding not only provides essential insight into oxygen stoichiometry control in high vacuum for understanding the fundamental properties of STO-based thin films and heterostructures, but expands the utility of pulsed laser epitaxy of other materials as well.
The duality principle in the presence of postselection
Authors: Leach Jonathan , Bolduc Eliot , Miatto Filippo M. , Piché Kevin , Leuchs Gerd , Boyd Robert W. 
Published: January 2016

AbstractThe duality principle, a cornerstone of quantum mechanics, limits the coexistence of wave and particle behaviours of quantum systems. This limitation takes a quantitative form when applied to the visibility "Equation missing" of interference fringes and predictability "Equation missing" of paths within a two-alternative system, which are bound by the inequality "Equation missing". However, if such a system is coupled to its environment, it becomes possible to obtain conditional measures of visibility and predictability, i.e. measures that are conditioned on the state of the environment. We show that in this case, the predictability and visibility values can lead to an apparent violation of the duality principle. We experimentally realize this apparent violation in a controlled manner by enforcing a fair-sampling-like loophole via postselection. This work highlights some of the subtleties that one can encounter while interpreting familiar quantities such as which-alternative information and visibility. While we concentrated on an extreme example, it is of utmost importance to realise that such subtleties might also be present in cases where the results are not obviously violating an algebraic bound, making them harder (but not any less crucial) to detect.
Phase-Controlled Iron Oxide Nanobox Deposited on Hierarchically Structured Graphene Networks for Lithium Ion Storage and Photocatalysis
Authors: Yun Sol , Lee Young-Chul , Park Ho Seok 
Published: January 2016

AbstractThe phase control, hierarchical architecturing and hybridization of iron oxide is important for achieving multifunctional capability for many practical applications. Herein, hierarchically structured reduced graphene oxide (hrGO)/α-Fe2O3 and γ-Fe3O4 nanobox hybrids (hrGO/α-Fe and hrGO/γ-Fe NBhs) are synthesized via a one-pot, hydrothermal process and their functionality controlled by the crystalline phases is adapted for energy storage and photocatalysis. The three-dimensionally (3D) macroporous structure of hrGO/α-Fe NBhs is constructed, while α-Fe2O3 nanoboxes (NBs) in a proximate contact with the hrGO surface are simultaneously grown during a hydrothermal treatment. The discrete α-Fe2O3 NBs are uniformly distributed on the surface of the hrGO/α-Fe and confined in the 3D architecture, thereby inhibiting the restacking of rGO. After the subsequent phase transition into γ-Fe3O4, the hierarchical structure and the uniform distribution of NBs are preserved. Despite lower initial capacity, the hrGO/α-Fe NBhs show better rate and cyclic performances than those of commercial rGO/α-Fe due to the uniform distribution of discrete α-Fe2O3 NBs and electronic conductivity, macroporosity and buffering effect of the hrGO for lithium ion battery anodes. Moreover, the catalytic activity and kinetics of hrGO/γ-Fe NBhs are enhanced for photo-Fenton reaction because of the uniform distribution of discrete γ-Fe3O4 NBs on the 3D hierarchical architecture.
Camouflage predicts survival in ground-nesting birds
Authors: Troscianko Jolyon , Wilson-Aggarwal Jared , Stevens Martin , Spottiswoode Claire N. 
Published: January 2016

AbstractEvading detection by predators is crucial for survival. Camouflage is therefore a widespread adaptation, but despite substantial research effort our understanding of different camouflage strategies has relied predominantly on artificial systems and on experiments disregarding how camouflage is perceived by predators. Here we show for the first time in a natural system, that survival probability of wild animals is directly related to their level of camouflage as perceived by the visual systems of their main predators. Ground-nesting plovers and coursers flee as threats approach and their clutches were more likely to survive when their egg contrast matched their surrounds. In nightjars – which remain motionless as threats approach – clutch survival depended on plumage pattern matching between the incubating bird and its surrounds. Our findings highlight the importance of pattern and luminance based camouflage properties and the effectiveness of modern techniques in capturing the adaptive properties of visual phenotypes.
An experimental proposal to test the physical effect of the vector potential
Authors: Wang Rui-Feng 
Published: January 2016

AbstractThere are two interpretations of the Aharonov–Bohm (A–B) effect. One interpretation asserts that the A–B effect demonstrates that the vector potential is a physical reality that can result in the phase shift of a moving charge in quantum mechanics. The other interpretation asserts that the phase shift of the moving charge results from the interaction energy between the electromagnetic field of the moving charge and external electromagnetic fields. This paper briefly reviews these two interpretations and analyzes their differences. In addition, a new experimental scheme is proposed to determine which interpretation is correct.
New α-glucosidase inhibitors from marine algae-derived Streptomyces sp. OUCMDZ-3434
Authors: Chen Zhengbo , Hao Jiejie , Wang Liping , Wang Yi , Kong Fandong , Zhu Weiming 
Published: January 2016

AbstractWailupemycins H (1) and I (2) with a new skeleton coupled two 6-(2-phenylnaphthalene-1-yl)pyrane-2-one nuclei to a –CH2– linkage were identified from the culture of Streptomyces sp. OUCMDZ-3434 associated with the marine algae, Enteromorpha prolifera. Compounds 1 and 2 are two new α-glucosidase inhibitors with the Ki/IC50 values of 16.8/19.7 and 6.0/8.3 μM, respectively. In addition, the absolute configurations of wailupemycins D (3) and E (4) are also resolved in this paper for the first time.
Boolean Models of Biological Processes Explain Cascade-Like Behavior
Authors: Chen Hao , Wang Guanyu , Simha Rahul , Du Chenghang , Zeng Chen 
Published: January 2016

AbstractBiological networks play a key role in determining biological function and therefore, an understanding of their structure and dynamics is of central interest in systems biology. In Boolean models of such networks, the status of each molecule is either “on” or “off” and along with the molecules interact with each other, their individual status changes from “on” to “off” or vice-versa and the system of molecules in the network collectively go through a sequence of changes in state. This sequence of changes is termed a biological process. In this paper, we examine the common perception that events in biomolecular networks occur sequentially, in a cascade-like manner, and ask whether this is likely to be an inherent property. In further investigations of the budding and fission yeast cell-cycle, we identify two generic dynamical rules. A Boolean system that complies with these rules will automatically have a certain robustness. By considering the biological requirements in robustness and designability, we show that those Boolean dynamical systems, compared to an arbitrary dynamical system, statistically present the characteristics of cascadeness and sequentiality, as observed in the budding and fission yeast cell- cycle. These results suggest that cascade-like behavior might be an intrinsic property of biological processes.
Donor-derived exosomes induce specific regulatory T cells to suppress immune inflammation in the allograft heart
Authors: Song Jiangping , Huang Jie , Chen Xiao , Teng Xiao , Song Zhizhao , Xing Yong , Wang Mangyuan , Chen Kai , Wang Zheng , Yang Pingchang , Hu Shengshou 
Published: January 2016

AbstractTo inhibit the immune inflammation in the allografts can be beneficial to organ transplantation. This study aims to induce the donor antigen specific regulatory T cells (Treg cell) inhibit the immune inflammation in the allograft heart. In this study, peripheral exosomes were purified from the mouse serum. A heart transplantation mouse model was developed. The immune inflammation of the allograft heart was assessed by histology and flow cytometry. The results showed that the donor antigen-specific T helper (Th)2 pattern inflammation was observed in the allograft hearts; the inflammation was inhibited by immunizing the recipient mice with the donor-derived exosomes. Purified peripheral exosomes contained integrin MMP1a; the latter induced CD4+ T cells to express Fork head protein-3 and transforming growth factor (TGF)-β via inhibiting the Th2 transcription factor, GATA binding protein 3, in CD4+ T cells. Administration with the donor-derived exosomes significantly prolonged the allograft heart survival. We conclude that the donor-derived peripheral exosomes have the capacity to inhibit the immune inflammation in the allograft heart via inducing specific Treg cells, implicating that administration with the donor-derived exosomes may be beneficial to cardiac transplantation.
Conformational Dynamics, Ligand Binding and Effects of Mutations in NirE an S-Adenosyl-L-Methionine Dependent Methyltransferase
Authors: Singh Warispreet , Karabencheva-Christova Tatyana G. , Black Gary W. , Ainsley Jon , Dover Lynn , Christov Christo Z. 
Published: January 2016

AbstractHeme d1, a vital tetrapyrrol involved in the denitrification processes is synthesized from its precursor molecule precorrin-2 in a chemical reaction catalysed by an S-adenosyl-L-methionine (SAM) dependent Methyltransferase (NirE). The NirE enzyme catalyses the transfer of a methyl group from the SAM to uroporphyrinogen III and serves as a novel potential drug target for the pharmaceutical industry. An important insight into the structure-activity relationships of NirE has been revealed by elucidating its crystal structure, but there is still no understanding about how conformational flexibility influences structure, cofactor and substrate binding by the enzyme as well as the structural effects of mutations of residues involved in binding and catalysis. In order to provide this missing but very important information we performed a comprehensive atomistic molecular dynamics study which revealed that i) the binding of the substrate contributes to the stabilization of the structure of the full complex; ii) conformational changes influence the orientation of the pyrrole rings in the substrate, iii) more open conformation of enzyme active site to accommodate the substrate as an outcome of conformational motions; and iv) the mutations of binding and active site residues lead to sensitive structural changes which influence binding and catalysis.
Characterization of Actin Filament Dynamics during Mitosis in Wheat Protoplasts under UV-B Radiation
Authors: Chen Huize , Han Rong 
Published: January 2016

AbstractEnhanced ultraviolet-B (UV-B) radiation is caused by the thinning ozone and affects photosynthesis and crop yield. Recently, UV-B radiation has been considered as an environmental signal that regulates plant growth. Elucidating the downstream effectors in UV-B-triggered pathways is of particular interest. Previous studies have shown that actin filaments (AFs) play many roles during cell physiological processes. However, the underlying response of AFs to UV-B radiation remains unclear. In this study, wheat protoplasts were isolated from 7-d-old leaves. The dynamics of AFs during mitosis were observed under different treatments. The protoplasts were treated with UV-B radiation, cytochalasin B (CB) and jasplakinolide (JAS). Ph-FITC labelling results revealed typical actin filament structures in the control group; AFs were rearranged under UV-B radiation. AFs polymerized into bundles during interphase, the preprophase band (PPB) structure was destroyed during prophase and the AFs gathered into plaques during metaphase in response to UV-B radiation. During anaphase and telophase, the distribution of AFs was dispersed. Pharmacologic experiments revealed that CB induced apoptosis and JAS induced nuclear division without cytokinesis in wheat protoplasts. These results indicated that AFs respond to UV-B radiation during mitosis, supplying evidence of UV-B signal transduction in plants.
Lower attention to daily environment: a novel cue for detecting chronic horses’ back pain?
Authors: Rochais C. , Fureix C. , Lesimple C. , Hausberger M. 
Published: January 2016

AbstractChronic pain is thought to affect patients’ cognitive functioning, including attention. Loss of attention is likely to have an impact on the execution of daily tasks, and, therefore, to have negative effects. However, relationships between chronic pain and cognitive deficits are still debated. Pre-clinical studies using laboratory animals prove useful to model pain-related cognitive impairment, but animal models had to predict effects in the real world. This study investigates attentional engagement of domestic horses by comparing observations in a home setting and evaluations of vertebral disorders. We found that lower attentional engagement and the level of back disorders were correlated. Two different evaluation techniques of the state of horses’ spines gave similar results. We suggest that novel animal models would prove useful for identifying spontaneous behaviours indicative of chronic pain. We suggest that more ethological studies in human patients’ home environments would help to improve our understanding of the processes involved. Finally, these results yield interesting indications for evaluating animal welfare, as attentional engagement could become a reliable indicator of chronic pain and thus a useful tool for identification of suffering individuals.
Lanatoside C Promotes Foam Cell Formation and Atherosclerosis
Authors: Shi Huairui , Mao Xiaobo , Zhong Yucheng , Liu Yuzhou , Zhao Xiaoqi , Yu Kunwu , Zhu Ruirui , Wei Yuzhen , Zhu Jianghao , Sun Haitao , Mao Yi , Zeng Qiutang 
Published: January 2016

AbstractLanatoside C’s impact on atherosclerosis is poorly understood. The present study was conducted to determine whether lanatoside C affects the development of atherosclerosis in apolipoprotein E-deficient (ApoE–/–) mice. ApoE–/– mice were administered either phosphate-buffered saline (PBS) containing 0.1% DMSO (the vehicle control group) or lanatoside C at low (1 mg/kg per day) or high (2 mg/kg per day) doses and fed a Western diet for 12 weeks. Lanatoside C dose-dependently aggravated the development of atherosclerosis in the ApoE–/– mice compared with the vehicle control group. In an effort to determine the mechanism by which lanatoside C increased atherosclerosis, we found that lanatoside C significantly promoted the uptake of oxidised low-density lipoprotein (oxLDL) and increased foam-cell formation by upregulation of scavenger receptor class A (SR-A) and the class B scavenger receptor (CD36) in macrophages. Meanwhile, the effects of lanatoside C were abolished using small interfering RNA (siRNA) inhibition of peroxisome proliferator-activated receptors β/δ (PPARβ/δ). Overall, our data demonstrate that lanatoside C aggravates the development of atherosclerosis by inducing PPARβ/δ expression, which mediates upregulation of SR-A and CD36 and promotes oxLDL uptake and foam-cell formation.
200‐GHz CMOS amplifier with 9‐dB noise figure for atmospheric remote sensing
Authors: Varonen M. , Safaripour A. , Parveg D. , Kangaslahti P. , Gaier T. , Hajimiri A. 
Published: January 2016

The feasibility of using CMOS technology for RF amplification in atmospheric remote sensing receiver is studied. The design and measurement results of a 200‐GHz low‐noise amplifier which is fabricated using a 32‐nm SOI CMOS technology are presented. The 8‐stage amplifier in a common‐source configuration achieves a 9‐dB noise figure and 25‐dB gain with a power consumption of 33 mW.
Molar fraction stability in dynamic preparation of reference trace gas mixtures
Authors: Demichelis Alessia , Sassi Guido , Lecuna Maricarmen , Sassi Maria Paola 
Published: January 2016

Volatile Organic Compounds (VOC) at trace level are common indicators for climate change and ambient air quality. The calibration of detection systems at trace level needs VOC mixtures at nmol/mol level with 5% uncertainty, the dynamic preparation can fulfil it. A source of uncertainty for dynamic generation is the stability of the VOC molar fraction averaged on the sampling time. A general method for the calculation of the relevance of the stability contribution to the total uncertainty is here proposed. VOC molar fraction is here considered as a composed quantity. Threshold values for stability contribution were set to consider it either as negligible and relevant. The stability of the operative quantities of a device, for the dynamic preparation of accurate VOC reference mixtures, was measured at the working conditions by the Allan deviation algorithm. Stabilities of operative quantities were composed to calculate the VOC molar fraction stability and their contribution to total uncertainty of VOC molar fraction. The stability of the air inlet pressure gave the main stability contribution. The stability contribution to the total uncertainty resulted to be negligible for observation times below 60 min for VOC molar fraction uncertainty of 2% at 30 nmol/mol level.
Die TIDieR Checkliste und Anleitung – ein Instrument für eine verbesserte Interventionsbeschreibung und Replikation
Authors: Hoffmann T. , Glasziou P. , Boutron I. , Milne R. , Perera R. , Moher D. , Altman D. , Barbour V. , Macdonald H. , Johnston M. , Lamb S. , Dixon-Woods M. , McCulloch P. , Wyatt J. , Chan A.-W. , Michie S. 
Published: January 2016

Zusammenfassung

Nur wenn Interventionsbeschreibungen vollständig veröffentlicht sind, können Kliniker und Patienten Interventionen, die sich als nützlich erwiesen haben, verlässlich umsetzen und andere Forscher die Studienergebnisse replizieren oder darauf aufbauen. Die Qualität von Interventionsbeschreibungen in wissenschaftlichen Publikationen ist bemerkenswert gering. Um die Vollständigkeit der Berichterstattung und damit die Replizierbarkeit von Interventionen zu verbessern, entwickelte eine internationale Gruppe von Experten und Interessensvertretern die Checkliste zur Interventionsbeschreibung und Replikation (TIDieR). Der Prozess beinhaltete eine Literaturrecherche zu relevanten Checklisten und wissenschaftlichen Untersuchungen, eine Delphi-Umfrage mit internationalen Experten zur Steuerung der Item-Auswahl und eine Expertenkonferenz. Die daraus resultierende 12-Item-TIDieR Checkliste (Bezeichnung, Warum, Was (Materialien), Was (Verfahren), Wer intervenierte, Wie, Wo, Wann und Wieviel, Anpassungen, Modifikationen, Wie gut (geplante Durchführungskontrolle), Wie gut (tatsächliche Durchführung)) ist eine Erweiterung des CONSORT 2010 Statements (Item 5) und des SPIRIT 2013 Statements (Item 11). Während der Fokus der Checkliste auf klinischen Studien liegt, kann die erweiterte Anleitung bei allen evaluativen Studiendesigns herangezogen werden. Dieser Artikel präsentiert die TIDieR Checkliste und Anleitung mit Erklärung und Erläuterung jedes einzelnen Items sowie Beispielen guter Berichterstattung. Die TIDieR Checkliste und Anleitung sollen das Berichten von Interventionen verbessern und Autoren eine Hilfe bieten, die Berichterstattung ihrer Interventionen zu strukturieren, Gutachtern und Herausgebern, die Beschreibungen zu beurteilen und Lesern, die Informationen zu nutzen.
Reexamining the acquisition of null subject pronouns in a second language
Authors: Clements Maria , Domínguez Laura 
Published: January 2016

AbstractThis study re-examines the L2 acquisition of referential and pragmatic properties of null and overt subject pronouns by advanced English learners of Spanish under the assumption that both forms display levels of complexity at the syntax–pragmatics interface. Our main hypothesis is that null subjects should be as difficult to acquire as overt subjects, challenging current generative accounts (e.g., the Interface Hypothesis) in which the acquisition of null subjects is problem-free. Data obtained by a group of 20 advanced English speakers of Spanish in aPicture Verification Taskand aContext-Matching Preference Taskcorroborate this hypothesis. Results show that L2 speakers over-accept null subjects and find it difficult to reject them when an overt pronoun is preferred by the controls. We propose that they may be using null subjects as a default form as they have an incomplete knowledge of the pragmatic constraints governing the use ofproin context.
Bearing performance as a function of structure and heat treatment
Authors: Blass T. , Dinkel M. , Trojahn W. 
Published: January 2016

The rings of rolling bearings are either through hardened with a martensitic or bainitic structure or case hardened. They differ in cleanliness, structure and residual stresses. The over-rolling fatigue resistance mirrors these differences. Typical results are given for over-rolling conditions causing classical fatigue, surface-initiated failure or failure by white etching cracking. This paper is part of a Themed Issue on Recent developments in bearing steels.