bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feral swine Sus scrofa: a new threat to the remaining breeding wetlands of the Vulnerable reticulated flatwoods salamander Ambystoma bishopi
Authors: Jones Kelly C. , Gorman Thomas A. , Rincon Brandon K. , Allen John , Haas Carola A. , Engeman Richard M. 
Published: February 2017

AbstractFeral swine Sus scrofa have been implicated as a major threat to sensitive habitats and ecosystems as well as threatened wildlife. Nevertheless, direct and indirect impacts on threatened species (especially small, fossorial species) are not well documented. The decline of the U.S. federally endangered reticulated flatwoods salamander Ambystoma bishopi, categorized as Vulnerable on the IUCN Red List, has been rapid and there are few remaining breeding locations for this species. The flatwoods salamander depends on complex herbaceous vegetation in all life stages, including eggs, larvae and adults. Historically sets of hog tracks have been observed only occasionally in the vicinity of monitored reticulated flatwoods salamander breeding wetlands, and damage to the wetlands had never been recorded. However, during the autumn–winter breeding season of 2013–2014 we observed a large increase in hog sign, including extensive rooting damage, in known flatwoods salamander breeding wetlands. Our objective was to assess the amount of hog sign and damage in these wetlands and to take corrective management actions to curb additional impacts. Of 28 wetlands surveyed for hog sign, presence was recorded at 68%, and damage at 54%. Of the 11 sites known to be occupied by flatwoods salamanders in 2013–2014, 64% had presence, and 55% had damage. We found that regular monitoring of disturbance in wetland habitats was a valuable tool to determine when intervention was needed and to assess the effectiveness of intervention. Habitat damage caused by feral hogs poses a potentially serious threat to the salamanders, which needs to be mitigated using methods to control and exclude hogs from this sensitive habitat.
Within-subject neural reactivity to reward and threat is inverted in young adolescents
Authors: Thomason M. E. , Marusak H. A. 
Published: February 2017

BackgroundAs children mature, they become increasingly independent and less reliant on caregiver support. Changes in brain systems are likely to stimulate and guide this process. One mechanistic hypothesis suggests that changes in neural systems that process reward and threat support the increase in exploratory behavior observed in the transition to adolescence. This study examines the basic tenets of this hypothesis by performing functional magnetic resonance imaging (fMRI) during well-established reward and threat processing tasks in 40 children and adolescents, aged 9–15 years.MethodfMRI responses in the striatum and amygdala are fit to a model predicting that striatal reward and amygdala threat-responses will be unrelated in younger participants (aged 9–12 years), while older participants (aged 13–15 years) will differentially engage these structures.ResultsOur data are consistent with this model. Activity in the striatum and amygdala are comparable in younger children, but in older children, they are inversely related; those more responsive to reward show a reduced threat-response. Analyses testing age as a continuous variable yield consistent results. In addition, the proportion of threat to reward-response relates to self-reported approach behavior in older but not younger youth, exposing behavioral relevance in the relative level of activity in these structures.ConclusionsResults are consistent with the notion that both individual and developmental differences drive reward-seeking behavior in adolescence. While these response patterns may serve adaptive functions in the shift to independence, skew in these systems may relate to increased rates of emotional psychopathology and risk-taking observed in adolescence.
The English Cassinese (1611-50)
Authors: Lunn David 
Published: February 2017

The Englishmen who were members of the Italian or Cassinese Benedictine Congregation in the seventeenth century have been neglected, mainly because, unlike the Englishmen who joined Spanish monasteries, the Cassinese did not perpetuate themselves and thus did not produce historians who would keeptheir memory alive. This is a pity, since several of them are important figures: Robert Gregory Sayer (1560-1602), who gained an international reputation as a moral theologian, which, it has been claimed, remains unrivalled among Englishmen; Roland Thomas Preston (1567-1647), whose prolific literaryoutput against the papal deposing power at least assures him of a place in the history of Anglo-Gallicanism; and Robert Anselm Beech (1568-1634), who was the agent in Rome who negotiated the setting up of the Benedictine mission to England and defended it against attack.The Anglo-Italians failed to perpetuate themselves because in 1616 they refused to unite with the other English monks, mainly in order to remain loyal to Preston, whom the others condemned for his writings. This article deals, first, with their attempt to found a community of their own at Paris, and, then, with their final years as a group. These two episodes have not been noticed before, and they have to be reconstructed entirely from manuscripts in the archives of the Archbishop of Westminster, the Abbey of S. Pietro at Perugia, the Congregation ‘de Propaganda Fide’ and the Vatican Library.
Twenty-Five Years of Recusant History
Authors: Allison A.F. , Rogers D.M. 
Published: February 2017

Fifteen years ago the present writers published a survey entitled ‘Ten Years of Recusant History’ (January issue, 1961). That article briefly described how this journal had evolved during its first ten years from a publication designed to supplement existing biographical works into ‘a periodical that would lay the foundations of a general history of Catholicism in these islands since the Reformation’.This is a statement of aims which, from the vantage point of a quarter of a century’s experience, we see no reason to alter. While much excellent material, derived mainly from local records of all sorts, now appears in the flourishing crop of county recusant periodicals which have sprung up in our wake, the pages of Recusant History continue to offer extended space for the publication of long articles, or even series of articles, treating specific topics in full depth. We still believe that definitive studies of this sort, with full apparatus of sources and references, must form the necessary groundwork for any reliable general histories to be written in the future.Such topics as we surveyed in 1961, for example biographies, family histories, recusant bibliography, and those wider questions concerning the status and fortunes of the whole Catholic body, have continued to be well represented in our pages during the ensuing fifteen years. But there has been growth as well as continuity. Looking back over the whole corpus of material published between 1961 and the end of 1975, we observe with pleasure that the previously under-cultivated period from 1700 onwards has received an amount of scholarly attention which fifteen years ago we did not dare to expect. Indeed, it has now been generally accepted that the boundaries of recusant history as a subject stretch onwards in time beyond those centuries when recusancy was a crime on the statute book. By a similar process the connotation of the word ‘recusant’ has been widened. It is perhaps not too much to claim that, by the expanded range of its subject matter, this journal has gone far towards validating the use of the word ‘recusancy’ as a general term covering post-Reformation English Catholicism even in its widest ramifications. It remains our aim to continue to explore and document its history and culture in all their rich variety.
Zika in Rio de Janeiro: Assessment of basic reproduction number and comparison with dengue outbreaks
Authors: VILLELA D. A. M. , BASTOS L. S. , DE CARVALHO L. M. , CRUZ O. G. , GOMES M. F. C. , DUROVNI B. , LEMOS M. C. , SARACENI V. , COELHO F. C. , CODEÇO C. T. 
Published: February 2017

SUMMARYZika virus infection was declared a public health emergency of international concern in February 2016 in response to the outbreak in Brazil and its suspected link with congenital anomalies. In this study, we use notification data and disease natural history parameters to estimate the basic reproduction number (R0) of Zika in Rio de Janeiro, Brazil. We also obtain estimates of R0 of dengue from time series of dengue cases in the outbreaks registered in 2002 and 2012 in the city, when DENV-3 and DENV-4 serotypes, respectively, had just emerged. Our estimates of the basic reproduction number for Zika in Rio de Janeiro based on surveillance notifications (R0 = 2·33, 95% CI: 1·97–2·97) were higher than those obtained for dengue in the city (year 2002: R0 = 1·70 [1·50–2·02]; year 2012: R0 = 1·25 [1·18–1·36]). Given the role of Aedes aegypti as vector of both the Zika and dengue viruses, we also derive R0 of Zika as a function of both dengue reproduction number and entomological and epidemiological parameters for dengue and Zika. Using the dengue outbreaks from previous years allowed us to estimate the potential R0 of Zika. Our estimates were closely in agreement with our first Zika's R0 estimation from notification data. Hence, these results validate deriving the potential risk of Zika transmission in areas with recurring dengue outbreaks. Whether transmission routes other than vector-based can sustain a Zika epidemic still deserves attention, but our results suggest that the Zika outbreak in Rio de Janeiro emerged due to population susceptibility and ubiquitous presence of Ae. aegypti.
Call-by-name extensionality and confluence
Authors: JOHNSON-FREYD PHILIP , DOWNEN PAUL , ARIOLA ZENA M. 
Published: February 2017

AbstractDesigning rewriting systems that respect functional extensionality for call-by-name languages with effects turns out to be surprisingly challenging. Simply interpreting extensional laws like η as reduction rules easily breaks confluence. We explore these issues in the setting of a sequent calculus. Building on an insight that appears in different aspects of the theory of call-by-name functional languages—confluent rewriting for two independent control calculi and sound continuation-passing style transformations—we give a confluent reduction system for lazy extensional functions. Finally, we consider limitations to this approach when used for strict evaluation and types beyond functions.
Structure and impact of cattle manure trade in crop–livestock systems of Vietnam
Authors: McRoberts Keenan C. , Nicholson Charles F. , Parsons David , Nam Le Van , Ba Nguyen Xuan , Ketterings Quirine M. , Cherney Debbie J. R. 
Published: February 2017

AbstractCattle manure value chains play important biological and economic roles in smallholder crop–livestock systems in developing countries, but relative to other livestock products our understanding of the nature and impact of manure sales is limited. In regions with an active manure trade, farmers face a choice between manure use on-farm and sales, which affects nutrient flows and participant incomes. We analyzed the manure value chain operating in south-central Vietnam as an example of the function and role of manure trade in crop–livestock systems. Lowland cattle farmers sell manure through a network of chain participants, including small-scale collectors, lowland and highland traders, to pepper, coffee, dragon fruit and rubber farms in the central highlands and southeast coast. We collected and summarized quantitative data (e.g., manure-related labor, manure transactions, and fertilizer and manure use) gathered in semi-structured interviews with value-chain participants [lowland cattle owners (n = 101), traders (n = 27) and end users (n = 72)]. Lowland cattle owners were selected by stratified random sampling, and subsequent participants were identified in preceding interviews. One key finding concerns the seasonality of the manure value chain: most manure flowed between February and August (lowland dry season and period of peak highland demand) from lowland communes to highland coffee and pepper farms for use as organic soil amendments. Fewer sales occurred, at a lower price, to southeast coastal dragon fruit farms and rubber companies. Value addition to manure occurred via drying, bagging, collection, transport and composting. The presence of local traders facilitated market sales for smallholder cattle owners, and prices through the value chain generally reflected costs for value addition. The geographic distribution of cattle relative to agricultural land influenced the flow of manure, with net outflows from regions with higher animal density to regions with lower density and higher value crop production. Manure trade was an important source of supplementary income for farmers and a primary livelihood activity for traders. Value chain participant net incomes ranged from near US$100 yr−1 for lowland farmers to over US$13,000 yr−1 for traders, and returns to labor were just over US$0.50 h−1 for lowland farmers and US$2 h−1 for traders. The quantitative information generated during our descriptive assessment provides an important first step toward manure value chain improvement, indicates survey methods that can be applied in other areas, and identifies next steps necessary to evaluate chain evolution and resilience.
MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells
Authors: Ito Keisuke , Eguchi Yutaka , Imagawa Yusuke , Akai Shuji , Mochizuki Hideki , Tsujimoto Yoshihide 
Published: February 2017

AbstractRegulation of cell death is potentially a powerful treatment modality for intractable diseases such as neurodegenerative diseases. Although there have been many reports about the possible involvement of various types of cell death in neurodegenerative diseases, it is still unclear exactly how neurons die in patients with these diseases, thus treatment strategies based on cell death regulation have not been established yet. To obtain some insight into the mechanisms of cell death involved in neurodegenerative diseases, we studied the effect of 1-methyl-4-phenylpyridinium (MPP+) on the human neuroblastoma cell line SH-SY5Y (a widely used model of Parkinson’s disease). We found that MPP+ predominantly induced non-apoptotic death of neuronally differentiated SH-SY5Y cells. This cell death was strongly inhibited by necrostatin-1 (Nec-1), a necroptosis inhibitor, and by an indole-containing compound (3,3′-diindolylmethane: DIM). However, it occurred independently of receptor-interacting serine/threonine-protein kinase 1/3 (RIP1/RIP3), indicating that this form of cell death was not necroptosis. MPP+-induced cell death was also inhibited by several inhibitors of ferroptosis, including ferrostatin-1 (Fer-1). Although MPP+-induced death and ferroptosis shared some features, such as occurrence of lipid peroxidation and inhibition by Fer-1, MPP+-induced death seemed to be distinct from ferroptosis because MPP+-induced death (but not ferroptosis) was inhibited by Nec-1, was independent of p53, and was accompanied by ATP depletion and mitochondrial swelling. Further investigation of MPP+-induced non-apoptotic cell death may be useful for understanding the mechanisms of neuronal loss and for treatment of neurodegenerative diseases such as Parkinson’s disease.
Antitumor effects of naturally occurring cardiac glycosides convallatoxin and peruvoside on human ER+ and triple-negative breast cancers
Authors: Kaushik Vivek , Azad Neelam , Yakisich Juan Sebastian , Iyer Anand Krishnan V 
Published: February 2017

AbstractBreast cancer is second most prevalent cancer in women, and the second only to lung cancer in cancer-related deaths. It is a heterogeneous disease and has several subtypes based on the presence or absence of hormone receptors and/or human epidermal growth factor receptor 2 (HER2). Hormone receptor-positive and HER2-enriched cancers can be targeted using hormone and HER2-targeting therapies such as trastuzumab or lapatinib. However, triple-negative breast cancers (TNBCs) do not express any of the receptors and therefore are resistant to most targeted therapies, and cytotoxic chemotherapies are the only viable option available for the treatment of TNBCs. Recently, cardiac glycosides (CGs) have emerged as potential anticancer agents that impart their antiproliferative effect by targeting multiple pathways. In this study our aim was to evaluate anticancer effects of two naturally occurring CGs, Convallatoxin (CT) and Peruvoside (PS), on ER+ and TNBCs cells. CT and PS demonstrated dose- and time-dependent cytotoxic effect on MCF-7 cells, which was further supported by loss of colony formation on drug treatment. CT and PS arrested MCF-7 cells in the G0/G1 phase and reduced the viability of MCF-7-derived mammospheres (MMs). Interestingly, while CT and PS imparted cell death in TNBCs cells from both Caucasians (MDA-MB-231 cells) and African Americans (MDA-MB-468 cells) in a dose- and time-dependent manner, the drugs were much more potent in MDA-MB-468 as compared with TNBC MDA-MB-231 cells. Both drugs significantly inhibited migration and invasion of both MCF-7 and MDA-MB-468 cells. An assessment of intracellular pathways indicated that both drugs were able to modulate several key cellular pathways such as EMT, cell cycle, proliferation and cell death in both cell types. Our data suggest a promising role for CGs in breast cancer treatment specifically in targeting TNBCs derived from African Americans, and provides impetus for further investigation of the anticancer potential of this class of drugs.
Optical screw-wrench for microassembly
Authors: Köhler Jannis , Ksouri Sarah Isabelle , Esen Cemal , Ostendorf Andreas 
Published: February 2017

AbstractFor future micro- and nanotechnologies, the manufacturing of miniaturized, functionalized, and integrated devices is indispensable. In this paper, an assembly technique based on a bottom-up strategy that enables the manufacturing of complex microsystems using only optical methods is presented. A screw connection is transferred to the micrometer range and used to assemble screw- and nut-shaped microcomponents. Micro-stereolithography is performed by means of two-photon polymerization, and microstructures are fabricated and subsequently trapped, moved, and screwed together using optical forces in a holographic optical tweezer set-up. The design and construction of interlocking microcomponents and the verification of a stable and releasable joint form the main focus of this paper. The assembly technique is also applied to a microfluidic system to enable the pumping or intermixing of fluids on a microfluidic chip. This strategy not only enables the assembly of microcomponents but also the combination of different materials and features to form complex hybrid microsystems.
DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs
Authors: Rossiello Francesca , Aguado Julio , Sepe Sara , Iannelli Fabio , Nguyen Quan , Pitchiaya Sethuramasundaram , Carninci Piero , d’Adda di Fagagna Fabrizio 
Published: February 2017

AbstractThe DNA damage response (DDR) is a set of cellular events that follows the generation of DNA damage. Recently, site-specific small non-coding RNAs, also termed DNA damage response RNAs (DDRNAs), have been shown to play a role in DDR signalling and DNA repair. Dysfunctional telomeres activate DDR in ageing, cancer and an increasing number of identified pathological conditions. Here we show that, in mammals, telomere dysfunction induces the transcription of telomeric DDRNAs (tDDRNAs) and their longer precursors from both DNA strands. DDR activation and maintenance at telomeres depend on the biogenesis and functions of tDDRNAs. Their functional inhibition by sequence-specific antisense oligonucleotides allows the unprecedented telomere-specific DDR inactivation in cultured cells and in vivo in mouse tissues. In summary, these results demonstrate that tDDRNAs are induced at dysfunctional telomeres and are necessary for DDR activation and they validate the viability of locus-specific DDR inhibition by targeting DDRNAs.
Connecting genetic risk to disease end points through the human blood plasma proteome
Authors: Suhre Karsten , Arnold Matthias , Bhagwat Aditya Mukund , Cotton Richard J. , Engelke Rudolf , Raffler Johannes , Sarwath Hina , Thareja Gaurav , Wahl Annika , DeLisle Robert Kirk , Gold Larry , Pezer Marija , Lauc Gordan , El-Din Selim Mohammed A. , Mook-Kanamori Dennis O. , Al-Dous Eman K. , Mohamoud Yasmin A. , Malek Joel , Strauch Konstantin , Grallert Harald , Peters Annette , Kastenmüller Gabi , Gieger Christian , Graumann Johannes 
Published: February 2017

AbstractGenome-wide association studies (GWAS) with intermediate phenotypes, like changes in metabolite and protein levels, provide functional evidence to map disease associations and translate them into clinical applications. However, although hundreds of genetic variants have been associated with complex disorders, the underlying molecular pathways often remain elusive. Associations with intermediate traits are key in establishing functional links between GWAS-identified risk-variants and disease end points. Here we describe a GWAS using a highly multiplexed aptamer-based affinity proteomics platform. We quantify 539 associations between protein levels and gene variants (pQTLs) in a German cohort and replicate over half of them in an Arab and Asian cohort. Fifty-five of the replicated pQTLs are located in trans. Our associations overlap with 57 genetic risk loci for 42 unique disease end points. We integrate this information into a genome-proteome network and provide an interactive web-tool for interrogations. Our results provide a basis for novel approaches to pharmaceutical and diagnostic applications.
Genome-wide identification of splicing QTLs in the human brain and their enrichment among schizophrenia-associated loci
Authors: Takata Atsushi , Matsumoto Naomichi , Kato Tadafumi 
Published: February 2017

AbstractDetailed analyses of transcriptome have revealed complexity in regulation of alternative splicing (AS). These AS events often undergo modulation by genetic variants. Here we analyse RNA-sequencing data of prefrontal cortex from 206 individuals in combination with their genotypes and identify cis-acting splicing quantitative trait loci (sQTLs) throughout the genome. These sQTLs are enriched among exonic and H3K4me3-marked regions. Moreover, we observe significant enrichment of sQTLs among disease-associated loci identified by GWAS, especially in schizophrenia risk loci. Closer examination of each schizophrenia-associated loci revealed four regions (each encompasses NEK4, FXR1, SNAP91 or APOPT1), where the index SNP in GWAS is in strong linkage disequilibrium with sQTL SNP(s), suggesting dysregulation of AS as the underlying mechanism of the association signal. Our study provides an informative resource of sQTL SNPs in the human brain, which can facilitate understanding of the genetic architecture of complex brain disorders such as schizophrenia.