bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signal peptide hydrophobicity modulates interaction with the twin-arginine translocase
Authors: Huang Qi , Palmer Tracy 
Published: May 2017

AbstractThe general secretory pathway (Sec) and twin-arginine translocase (Tat) operate in parallel to export proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of plant chloroplasts. Substrates are targeted to their respective machineries by N-terminal signal peptides that share a common tripartite organization, however Tat signal peptides harbor a conserved and almost invariant arginine pair that are critical for efficient targeting to the Tat machinery. Tat signal peptides interact with a membrane-bound receptor complex comprised of TatB and TatC components, with TatC containing the twin-arginine recognition site. Here we isolated suppressors in the signal peptide of the Tat substrate, SufI, that restored Tat transport in the presence of inactivating substitutions in the TatC twin-arginine binding site. These suppressors increased signal peptide hydrophobicity, and co-purification experiments indicated that they restored binding to the variant TatBC complex. The hydrophobic suppressors could also act incisto suppress substitutions at the signal peptide twin-arginine motif that normally prevent targeting to the Tat pathway. Highly hydrophobic variants of the SufI signal peptide containing four leucine substitutions retained the ability to interact with the Tat system. The hydrophobic signal peptides of two Sec substrates, DsbA and OmpA, containing twin lysine residues, were shown to mediate export by the Tat pathway and to co-purify with TatBC. These findings indicate that there is unprecedented overlap between Sec and Tat signal peptides and that neither the signal peptide twin-arginine motif nor the TatC twin-arginine recognition site are essential mechanistic features for operation of the Tat pathway.ImportanceProtein export is an essential process in all prokaryotes, The Sec and Tat export pathways operate in parallel, with the Sec machinery transporting unstructured precursors and the Tat pathway transporting folded proteins. Proteins are targeted to the Tat pathway by N-terminal signal peptides that contain an almost invariant twin-arginine motif. Here we make the surprising discovery that the twin-arginines are not essential for recognition of substrates by the Tat machinery, and that this requirement can be bypassed by increasing the signal peptide hydrophobicity. We further show that signal peptides ofbona fideSec substrates can also mediate transport by the Tat pathway. Our findings suggest that key features of the Tat targeting mechanism have evolved to prevent mis-targeting of substrates to the Sec pathway rather than being a critical requirement for function of the Tat pathway.
Digital native or digital immigrant? Using intraorganizational resources to develop technological competence among older employees
Authors: Evans Robert D. 
Published: May 2017

Purpose
The purpose of this viewpoint article is to demonstrate that firms can utilize intraorganizational resources to aid in helping older workers become technologically competent. Through the use of these intraorganizational resources, firms can benefit from the transfer of technological knowledge between younger and older workers. A further benefit is cost savings associated with utilization of internal as opposed to external resources.

Design/methodology/approach
This paper is a viewpoint article which demonstrates and raises awareness of some elementary internal resources which can aid in the development of older workers’ technological competence.

Findings
There exists a marked difference between younger and older workers and the associated technological competence of each. Implementing a mentoring process which matches recent college graduates or younger workers, digital natives, with older workers, digital immigrants, who are either returning to the workforce or transitioning to jobs which require technological skills, can aid in knowledge transfer of proprietary and public software programs and has the potential to lead to improved employee relationships.

Originality/value
This paper addresses the development and utilization of internal as opposed to external resources in aiding older workers gaining technological competence. These workers may be returning to the workforce or transitioning to jobs which require more technological knowledge, and, by providing a system to support this transition, both, firms and employees can benefit.
Early success experience as a predictor of fast-track career growth
Authors: Pandya Saurabh 
Published: May 2017

Purpose
This paper aims to recommend an approach which can help ensure the fast-track career progression of employees.

Design/methodology/approach
The paper uses data from a larger study on leadership development practices in the context of rapid growth organizations. This was based on semi-structured interviews with 43 senior managers from medium-sized organizations in the Indian software services industry.

Findings
The study suggests that many respondents, who were from a pool of fast-track employees, had experienced some kind of success early on in their careers or particular roles. This was due to various reasons such as exciting opportunities, proximity to top management, a supportive boss, or challenging assignments.

Originality/value
The paper can be of immense help to organizations by helping them to plan their induction and onboarding activities more effectively, so as to provide new recruits with early success experience of some sort, which in turn will help them to fast-track their career growth.
Establishing yourself as a thought leader? Seven simple ideas to improve the quality of your thinking
Authors: Gibbins-Klein Mindy 
Published: May 2017

Purpose
To establish ourselves as true thought leaders, we need to develop our skills so that we hone and shape our thinking before communicating with others. This paper aims toexplore the importance of pushing your own thinking further and offers seven simple practices that will help take it to the next level.

Design/methodology/approach
The author draws on 18 years of experience as a thought leader strategist.

Findings
Seven tactics are presented which if followed can sharpen thinking and help establish someone as a thought leader in their field.

Originality/value
The organizational world is now connected on a minute-by-minute basis through technology, and particularly social media. All leaders need to focus on the quality of their thinking, how they share their thinking and how this sharing shapes their global reputation.
Financial inclusion, biometric identification and mobile: unlocking the JAM trinity
Authors: Ghosh Saibal 
Published: May 2017

Purpose
The purpose of the paper is to understand the interlinkage with financial inclusion and how it interacts with biometric identification. To investigate this in detail, the authors employ household-level data on India to examine the interlinkage among Prime Minister Jan Dhan Yojana (PMJDY) account, Aadhaar card and mobile telephony.

Design/methodology/approach
Given the survey data, the authors employ 3 stage least squares (3SLS) methodology to explore the association among these key variables, while controlling for other household, district and economy-wide factors.

Findings
The findings provide strong evidence of complementarity among these variables, with each tending to reinforce the other. This complementarity is reflected primarily in respect of PMJDY and Aadhaar, but much less so with regard to mobile telephony. Additionally, this complementarity is manifest more prominently in the long run, although it is much less so in the short run.

Originality/value
To the best of the authors’ knowledge, this is one of the earliest studies for India to systematically examine the Jan-Dhan-Aadhaar-Mobile (JAM) trinity.
Paying for education among households in Ghana
Authors: Iddrisu Abdul Malik , Danquah Michael , Quartey Peter 
Published: May 2017

Purpose
Using data from the sixth round of the Ghana Living Standards Survey, this paper aims to take a critical look at issues relating to the demand for education in Ghana.

Design/methodology/approach
In doing this, the paper develops a model for the determinants of household’s educational expenditure using the full sample of data and an income-quintile disaggregated model of the determinants of household’s educational expenditures. The study adopts robust empirical estimation techniques to estimate the model.

Findings
The paper finds that household resources importantly influence children’s educational expenditures with wealthier households spending proportionately more in educating their children than poorer households; large-sized households spend more in terms of educational expenses than small-sized households reflecting largely the quantity of education purchased, given that quality and contextual factors matters for educational investments. Strikingly, the findings show that female headship is a significant positive predictor of households’ demand for education. These findings provide valuable policy options relating to the goal of ensuring inclusive and quality education for all and promoting a lifelong learning by 2030.

Originality/value
While literature on the determinants of households’ educational expenditures abound, very few of this literature focuses on sub-Saharan Africa. Consequently, this study makes an important contribution to the literature by providing evidence on the determinants of households’ educational expenditure in the context of sub-Saharan Africa.
The influence of strategy and concurrent engineering on design for procurement
Authors: Arnette Andrew , Brewer Barry 
Published: May 2017

PurposeThe purpose of this paper is to examine the role of strategy and concurrent engineering (CE) in driving design for procurement (DFP) actions and results via the role of procurement professionals in new product development (NPD). The strategies of cost leadership, differentiation, and a hybrid approach are compared, and sequential NPD is compared to a CE approach within a DFP context.Design/methodology/approachANOVA was applied to survey data collected for a series of items capturing the activities and characteristics relating to procurement for a new product design, as well as the performance of the product compared to other design events in the firm.FindingsSeveral major findings were supported through the analysis. Product-level strategy played a limited role, at best, in driving the implementation of procurement activities and product performance. In contrast, high CE intensity was shown to improve procurement activity and product performance. The results were analyzed along the three dimensions of sustainability, and were especially strong for both environmental and economic-focused activities and performance.Practical implicationsManagers should work to integrate procurement early into NPD activities, ensure procurement uses strategy to drive decisions, and can use DFP initiatives from this research to implement a DFP program.Originality/valueThis research is one of the first attempts to empirically test design-for (DFX) approaches in NPD. It creates one of the first theoretical frameworks for DFX-related research.
Towards more efficient logistics: increasing load factor in a shipper’s road transport
Authors: Santén Vendela 
Published: May 2017

Purpose
Increasing load factor is crucial for transport efficiency and may benefit shippers because of its potential to reduce both environmental impact and transportation costs. The purpose of this paper is to explore how shippers can increase load factor in their road transport by identifying opportunities for logistics action and influences on load factor performance measures created by such opportunities.

Design/methodology/approach
A case study is performed of the outgoing goods flow from the central warehouse of a large retailer in Sweden. Data are collected from interviews with the shipper and its contracted freight forwarder, as well as from archival sources and visual observations, and applied to produce a framework.

Findings
Logistics actions that can increase load factor are identified and categorised according to packaging efficiency, loading efficiency and booking efficiency, all of which are linked to logistics variables and specific performance measures in the framework. Visual observations of volumetric load factor in vehicles indicate room for improvement via, for example, making lead times more flexible.

Practical implications
The framework’s principles can be used to support shippers in finding opportunities to increase load factor.

Originality/value
The framework clarifies the concept of load factor as a whole by explaining each logistics action’s contribution to increasing load factor, as well as the actions’ combined effect in the context of a shipper and its purchased transport share.
Improving supply chain flexibility and agility through variety management
Authors: Um Juneho 
Published: May 2017

PurposeA trade-off exists between product variety and supply chain (SC) performance. To help mitigate the impact on SC of increased product variety, the purpose of this paper is to examine how variety management activities including variety management strategy (VMS), supplier partnerships and close customer relationships affect SC flexibility and agility at different levels of customisation.Design/methodology/approachA survey-research methodology is employed using data from 363 manufacturing firms from the UK and South Korea. In particular, cluster analysis and structural equation modelling were used to evaluate the proposed model according to the level of customisation.FindingsThe results suggest that internal variety management strategy and external SC integration have a positive influence on SC flexibility and agility. Customer relationships and variety management strategies influence SC flexibility more than partnerships with suppliers whereas variety management strategies and partnerships with suppliers influence SC agility more than customer relationships. In fact, for external integration in particular, customer relationships influence SC flexibility (i.e. reaction capability) rather than agility (i.e. reaction time) whereas partnerships with suppliers influence SC agility rather than flexibility. In a high-customisation context, close customer relationships are the most effective way to increase SC flexibility, whereas partnerships with suppliers are the most effective way to increase SC agility. In a low-customisation context, a VMS and customer relationships are the most effective way to increase both SC flexibility and agility.Originality/valueThis paper suggests key variety management activities to aid managers to better manage product variety ambitions in SC under varying customisation profiles through internal and external approaches.
The past and future of supply chain collaboration: a literature synthesis and call for research
Authors: M. Ralston Peter , Richey R. Glenn , J. Grawe Scott 
Published: May 2017

Purpose
The purpose of this paper is to provide scholarly and practical benefits by detailing the past and suggesting a future research agenda for supply chain (SC) collaboration. A literature review is utilized to examine what has been investigated prior, and what remains to be analyzed, in order to assist today’s managers and researchers. The research expands the understanding of SC collaboration from a focal firm perspective while providing boundaries for future investigation and at the same time detailing the current state of collaboration to practitioners.

Design/methodology/approach
The current research utilizes a systematic review of the literature to shape a proposed research agenda on the topic of SC collaboration.

Findings
The paper provides insights into gaps in the literature as it pertains to SC collaboration. Specifically, the paper suggests investigating SC collaboration as it relates to perceived and real performance, understanding what know-how and commitment a focal firm must make to SC collaboration, and how to successfully navigate collaboration termination.

Research limitations/implications
This manuscript makes four specific contributions to the literature. First, it provides the first holistic graphic depiction of the central constructs employed in extant SC collaboration research. Next it examines three specific factor areas influencing collaboration. Overlooked issues are then revealed as suggestions for future research in SC collaboration. Finally, the method employed to conduct the systematic literature review can be used as a blueprint for future researchers in performing a similar exercise.

Practical implications
The current research seeks to provide a research agenda which meets the needs of today’s business managers.

Originality/value
The paper provides a suggested research agenda for SC collaboration.
Do corporate sustainable management activities improve customer satisfaction, word of mouth intention and repurchase intention? Empirical evidence from the shipping industry
Authors: Shin Youngran , Thai Vinh Van , Grewal Devinder , Kim Yulseong 
Published: May 2017

Purpose
As the concept of corporate sustainable management activities (CSMA) has become an increasingly important element of corporate management, much attention has been paid to its impact on customer relationship. Although there exists many studies concentrating mainly on the environment aspect of CSMA, only a few address the three dimensions of CSMA (environmental, social and economic) and examine their comprehensive impact on relationship marketing. In an attempt to fill this gap, the purpose of this paper is to empirically investigate the influence of CSMA on customer satisfaction (CS), word of mouth intention (WOMI) and repurchase intention (RI).

Design/methodology/approach
The data come from 214 shipping industry practitioners (shippers, freight forwarders and third party logistics service providers in South Korea). Structural equation modeling (SEM) is employed to test the impact of CSMA on CS, WOMI and RI.

Findings
Based on a survey conducted in South Korea, the findings indicate that environmental and economic aspects of CSMA play a significant role in enhancing CS and the enhanced CS positively affects WOMI and RI.

Research limitations/implications
As the field data were obtained from only one industry, future replication of the findings to other industries should consider industry-specific factors, if applicable.

Practical implications
The results highlight several implications for shipping industry practitioners to consider when establishing the strategy for effective corporate sustainable management to enhance CS.

Originality/value
This study is one of the first empirical attempts to explore how CSMA affects CS in the shipping industry. In addition, this paper empirically investigates the influence of CS on WOMI and RI.
Larger and better
Authors: Spielmann Nathalie 
Published: May 2017

PurposeWineries today are faced with the prospect of having to include environmental sustainability into their practices but implementation can be hard, complicated or even undesired. This research aims to examine firm features, specifically winery size and foreign direct investment, as potential sources of variability regarding environmental sustainability attitudes and practices.Design/methodology/approachQuestionnaires were administered via telephone interviews with 63 wineries in France. Production surface and wine activities in other countries were the independent variables examined as potentially predicting environmental sustainability attitudes and practices, leading to competitive positioning and perceived firm success.FindingsThe findings clearly show that bigger wineries are more likely to practice environmental sustainability, but they do not necessarily have more positive attitudes toward environmental sustainability. For winery managers, firm size and environmental sustainability practices interact because they are perceived to lead to competitive advantages such as augmented product quality and better innovations. Larger firms are also more sensitive to micro pressures emanating from customers, competitors and distributors regarding environmental sustainability. Finally, wineries engaging in foreign direct investments have more positive attitudes toward and engage in more environmental sustainability practices than firms that remain domestic.Originality/valueRather than comparing firms that are environmentally sustainable versus firms that are not, this research examined actual firm characteristics that may influence management’s propensity to engage in environmental sustainability practices. This research provides explanations for why there are augmented environmental sustainability practices by larger wineries and the sources of subjective norms encouraging larger wineries, versus smaller wineries, to practice environmental sustainability.
Beyond autism and technology: lessons from neurodiverse populations
Authors: Brosnan Mark , Holt Samantha , Yuill Nicola , Good Judith , Parsons Sarah 
Published: May 2017

Purpose
The purpose of this paper is to report on the sixth seminar in a seven-seminar series entitled, Innovative Technologies for Autism: Critical Reflections on Digital Bubbles, funded by the UK Economic and Social Research Council. The aim of this particular seminar was to reflect upon the implications from neurodiverse communities for the development of technology for autism.

Design/methodology/approach
Presentations from key researchers and parental perspectives are reviewed, highlighting contemporary issues in neurodiverse populations that have important implications for autism.

Findings
Whilst there are many conditions associated with autism, most commonly intellectual disability (learning difficulties), this is not reflected in research. In addition, for child-based research, researchers are at least a generation older than participants and have had different digital-childhoods. Involving neurodiverse populations within participatory design sessions can address both of these issues. Understanding the context of the issues that the participatory design sessions address is crucial for developing participatory design principles that extend from one condition to another. This includes understanding when findings based upon verbal populations can be extended to non-verbal populations.

Originality/value
This paper offers up-to-date insights into how design principles from one condition extend to different conditions. Universal interaction and neurodiversity HCI are considered. This is important within neurodiverse populations, especially given the high rates of additional conditions that are associated with autism. Whilst the majority of autism research has involved verbal populations, the benefits of technology can extend to non-verbal populations.