bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Rescue Under the Rubble: The Medical Aid in the First Hours After the Earthquake in Amatrice (Italy) on August 24, 2016
Authors: Blasetti Angelo Geremia , Petrucci Emiliano , Cofini Vincenza , Pizzi Barbara , Scimia Paolo , Pozone Tullio , Necozione Stefano , Fusco Pierfrancesco , Marinangeli Franco 
Published: December 2017

Specific Event Identifiersa. Event Type: Earthquake measuring 6.2 (SD=0.016) on the moment magnitude;b. Event Onset: August 24, 2016 - 03:36:32 CEST (01:36 UTC);c. Location of Event: Central Italy, in the town of Amatrice;d. Geographic Coordinates: latitude (DMS): 42°37′45.77″N; longitude (DMS): 13°17′18.14″E; elevation: 955 meters above sea-level;e. Dates: August 24, 2016 at 4:48 AM;f. Response Type: Medical Relief.AbstractOn August 24, 2016, an earthquake hit the town of Amatrice (Italy). This study aims to document the first medical aid provided to earthquake victims in Amatrice immediately following the earthquake.Patient data were collected and recorded during the first clinical evaluation and before definitive hospitalization. Blood gas tests were performed on survivors extricated from the rubble using the iSTAT (Abbott Point of Care Inc.; Princeton, New Jersey USA) handheld blood analyzer.Performing “victim-side” blood gas tests could provide concrete information to facilitate clinical evaluation and decision making when treating buried victims. After a natural disaster, it is essential to provide effective analgo-sedation to victims.BlasettiAG, PetrucciE, CofiniV, PizziB, ScimiaP, PozoneT, NecozioneS, FuscoP, MarinangeliF. First rescue under the rubble: the medical aid in the first hours after the earthquake in Amatrice (Italy) on August 24, 2016. Prehosp Disaster Med. 2018;33(1):109–113.
The complex resistomes of Paenibacillaceae reflect diverse antibiotic chemical ecologies
Authors: Pawlowski Andrew C , Westman Erin L , Koteva Kalinka , Waglechner Nicholas , Wright Gerard D 
Published: December 2017

Abstract
The ecology of antibiotic resistance involves the interplay of a long natural history of antibiotic production in the environment, and the modern selection of resistance in pathogens through human use of these drugs. Important components of the resistome are intrinsic resistance genes of environmental bacteria, evolved and acquired over millennia, and their mobilization, which drives dissemination in pathogens. Understanding the dynamics and evolution of resistance across bacterial taxa is essential to address the current crisis in drug-resistant infections. Here we report the exploration of antibiotic resistance in the Paenibacillaceae prompted by our discovery of an ancient intrinsic resistome in Paenibacillus sp. LC231, recovered from the isolated Lechuguilla cave environment. Using biochemical and gene expression analysis, we have mined the resistome of the second member of the Paenibacillaceae family, Brevibacillus brevis VM4, which produces several antimicrobial secondary metabolites. Using phylogenomics, we show that Paenibacillaceae resistomes are in flux, evolve mostly independent of secondary metabolite biosynthetic diversity, and are characterized by cryptic, redundant, pseudoparalogous, and orthologous genes. We find that in contrast to pathogens, mobile genetic elements are not significantly responsible for resistome remodeling. This offers divergent modes of resistome development in pathogens and environmental bacteria.
Activation of the c-Jun NH2-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-Jun
Authors: Fung To Sing , Liu Ding Xiang 
Published: December 2017

AbstractMitogen-activated protein kinases (MAPKs) are conserved protein kinases that regulate a variety of important cellular signaling pathways. Among them, c-Jun N-terminal kinases (JNK) are known to be activated by various environmental stresses including virus infections. Previously, activation of the JNK pathway has been detected in cells infected with several coronaviruses. However, detailed characterization of the pathway as well as its implication in host–virus interactions has not been fully investigated. Here we report that the JNK pathway was activated in cells infected with the avian coronavirus infectious bronchitis virus (IBV). Of the two known upstream MAPK kinases (MKK), MKK7, but not MKK4, was shown to be responsible for IBV-induced JNK activation. Moreover, knockdown and overexpression experiments demonstrated that JNK served as a pro-apoptotic protein during IBV infection. Interestingly, pro-apoptotic activity of JNK was not mediated via c-Jun, but involved modulation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl2). Taken together, JNK constitutes an important aspect of coronavirus–host interaction, along with other MAPKs.
Silica-grafted ionic liquids for revealing the respective charging behaviors of cations and anions in supercapacitors
Authors: Dou Qingyun , Liu Lingyang , Yang Bingjun , Lang Junwei , Yan Xingbin 
Published: December 2017

AbstractSupercapacitors based on activated carbon electrodes and ionic liquids as electrolytes are capable of storing charge through the electrosorption of ions on porous carbons and represent important energy storage devices with high power delivery/uptake. Various computational and instrumental methods have been developed to understand the ion storage behavior, however, techniques that can probe various cations and anions of ionic liquids separately remain lacking. Here, we report an approach to monitoring cations and anions independently by using silica nanoparticle-grafted ionic liquids, in which ions attaching to silica nanoparticle cannot access activated carbon pores upon charging, whereas free counter-ions can. Aided by this strategy, conventional electrochemical characterizations allow the direct measurement of the respective capacitance contributions and acting potential windows of different ions. Moreover, coupled with electrochemical quartz crystal microbalance, this method can provide unprecedented insight into the underlying electrochemistry.
Catastrophic disassembly of actin filaments via Mical-mediated oxidation
Authors: Grintsevich Elena E. , Ge Peng , Sawaya Michael R. , Yesilyurt Hunkar Gizem , Terman Jonathan R. , Zhou Z. Hong , Reisler Emil 
Published: December 2017

AbstractActin filament assembly and disassembly are vital for cell functions. MICAL Redox enzymes are important post-translational effectors of actin that stereo-specifically oxidize actin’s M44 and M47 residues to induce cellular F-actin disassembly. Here we show that Mical-oxidized (Mox) actin can undergo extremely fast (84 subunits/s) disassembly, which depends on F-actin’s nucleotide-bound state. Using near-atomic resolution cryoEM reconstruction and single filament TIRF microscopy we identify two dynamic and structural states of Mox-actin. Modeling actin’s D-loop region based on our 3.9 Å cryoEM reconstruction suggests that oxidation by Mical reorients the side chain of M44 and induces a new intermolecular interaction of actin residue M47 (M47-O-T351). Site-directed mutagenesis reveals that this interaction promotes Mox-actin instability. Moreover, we find that Mical oxidation of actin allows for cofilin-mediated severing even in the presence of inorganic phosphate. Thus, in conjunction with cofilin, Mical oxidation of actin promotes F-actin disassembly independent of the nucleotide-bound state.
Whole exome sequencing identified sixty-five coding mutations in four neuroblastoma tumors
Authors: Miller Aubrey L. , Garcia Patrick L. , Pressey Joseph G. , Beierle Elizabeth A. , Kelly David R. , Crossman David K. , Council Leona N. , Daniel Richard , Watts Raymond G. , Cramer Stuart L. , Yoon Karina J. 
Published: December 2017

AbstractNeuroblastoma is a pediatric tumor characterized by histologic heterogeneity, and accounts for ~15% of childhood deaths from cancer. The five-year survival for patients with high-risk stage 4 disease has not improved in two decades. We used whole exome sequencing (WES) to identify mutations present in three independent high-risk stage 4 neuroblastoma tumors (COA/UAB-3, COA/UAB -6 and COA/UAB -8) and a stage 3 tumor (COA/UAB-14). Among the four tumors WES analysis identified forty-three mutations that had not been reported previously, one of which was present in two of the four tumors. WES analysis also corroborated twenty-two mutations that were reported previously. No single mutation occurred in all four tumors or in all stage 4 tumors. Three of the four tumors harbored genes with CADD scores ≥20, indicative of mutations associated with human pathologies. The average depth of coverage ranged from 39.68 to 90.27, with >99% sequences mapping to the genome. In summary, WES identified sixty-five coding mutations including forty-three mutations not reported previously in primary neuroblastoma tumors. The three stage 4 tumors contained mutations in genes encoding protein products that regulate immune function or cell adhesion and tumor cell metastasis.
Tailored plasmon-induced transparency in attenuated total reflection response in a metal–insulator–metal structure
Authors: Matsunaga Kouki , Hirai Yusuke , Neo Yoichiro , Matsumoto Takahiro , Tomita Makoto 
Published: December 2017

AbstractWe demonstrated tailored plasmon-induced transparency (PIT) in a metal (Au)–insulator (SiO2)–metal (Ag) (MIM) structure, where the Fano interference between the MIM waveguide mode and the surface plasmon polariton (SPP) resonance mode induced a transparency window in an otherwise opaque wavenumber (k) region. A series of structures with different thicknesses of the Ag layer were prepared and the attenuated total reflection (ATR) response was examined. The height and width of the transparency window, as well as the relevant k-domain dispersion, were controlled by adjusting the Ag layer thickness. To confirm the dependency of PIT on Ag layer thickness, we performed numerical calculations to determine the electric field amplitude inside the layers. The steep k-domain dispersion in the transparency window is capable of creating a lateral beam shift known as the Goos–Hänchen shift, for optical device and sensor applications. We also discuss the Fano interference profiles in a ω − k two-dimensional domain on the basis of Akaike information criteria.
PiT2 regulates neuronal outgrowth through interaction with microtubule-associated protein 1B
Authors: Ma Xi-Xiang , Li Xiangyang , Yi Ping , Wang Cheng , Weng Jun , Zhang Li , Xu Xuan , Sun Hao , Feng Shenglei , Liu Kai , Chen Rui , Du Shiyue , Mao Xiao , Zeng Xiaomei , Zhang Luo-Ying , Liu Mugen , Tang Bei-Sha , Zhu Xiaojuan , Jin Shan , Liu Jing-Yu 
Published: December 2017

AbstractPiT2 is a member of the inorganic phosphate transporter family, and is extensively expressed in the nervous system. It was found that loop7 domain of PiT2 is not required for retroviral recognition and transport function. The exact functions of loop7 remain poorly understood. Here we show that loop7 of PiT2 is necessary for the transport of PiT2 protein to the cell surface. Further, loop7 is also related to the outgrowth of neurite in Neuro2A cells interacts with the light chain 1 of microtubule-associated protein 1B (MAP1B). PiT2 with mutated MAP1B binding sites affect neurite outgrowth whereas Pi transport function deficient mutants of PiT2 do not. We also show that Drosophila dPiT interacts with microtubule-associated protein Futsch, and dPiT is crucial for the normal development of neuromuscular junctions (NMJs). These results indicate that PiT2 might participate in the regulation of neuronal outgrowth by interacting with MAP1B and independently of its Pi transport function in the nervous system.
Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle
Authors: Ikeda Mitsumi , Matsuyama Shuichi , Akagi Satoshi , Ohkoshi Katsuhiro , Nakamura Sho , Minabe Shiori , Kimura Koji , Hosoe Misa 
Published: December 2017

AbstractIsoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-strand break near the mutation site. CRISPR/Cas9 and donor DNA that contained a synonymous codon for the correct amino acid and an Aequorea coerulescens Green Fluorescent Protein (AcGFP) cassette with a piggyBac transposase recognition site at both ends were introduced into bovine fetal fibroblast (BFF) cells isolated from a homozygous mutant calf. Recombinant cells were enriched on the basis of expression of AcGFP, and two cell lines that contained the repaired allele were subcloned. We generated somatic cell nuclear transfer (SCNT) embryos from the repaired cells and transferred 22 blastocysts to recipient cows. In total, five viable fetuses were retrieved at Days 34 and 36. PiggyBac transposase mRNA was introduced into BFF cells isolated from cloned foetuses and AcGFP-negative cells were used for second round of cloning. We transferred nine SCNT embryos to recipient cows and retrieved two fetuses at Day 34. Fetal genomic DNA analysis showed correct repair of the IARS mutation without any additional DNA footprint.
Optical Bloch oscillation and Zener tunneling in the fractional Schrödinger equation
Authors: Zhang Yiqi , Wang Rong , Zhong Hua , Zhang Jingwen , Belić Milivoj R. , Zhang Yanpeng 
Published: December 2017

AbstractWe demonstrate optical Bloch oscillation (OBO) and optical Zener tunneling (OZT) in the fractional Schrödinger equation (FSE) with periodic and linear potentials, numerically and theoretically. We investigate in parallel the regular Schrödinger equation and the FSE, by adjusting the Lévy index, and expound the differences between the two. We find that the spreading of the OBO decreases in the fractional case, due to the diminishing band width. Increasing the transverse force, due to the linear potential, leads to the appearance of OZT, but this process is suppressed in the FSE. Our results indicate that the adjustment of the Lévy index can effectively control the emergence of OBO and OZT, which can inspire new ideas in the design of optical switches and interconnects.
Change in Land Use and Evapotranspiration in the Manas River Basin, China with Long-term Water-saving Measures
Authors: Yang Guang , Xue Lianqing , He Xinlin , Wang Cui , Long Aihua 
Published: December 2017

AbstractWidespread application of water-saving measures, especially advanced drip irrigation technologies, may significantly impact on the land use, and further potentially alter regional ecological environments in an arid area. In this study, the remote sensing and geographic information system technology were used to analyze the LANDSAT images (1976–2015) and the MOD16 evapotranspiration data (2000–2015) in the Manas River Basin (MRB), China where the water-saving technologies have experienced the past 40 years. Our results show that the area of the cultivated land was approximately doubled from 1976 to 2015 with a dynamic degree of cultivated land ranging from 1.7% to 4%. The reclamation rates were estimated at 9.5% in 1976 and 21.8% in 2015 and the comprehensive index of land use degree shows an increasing trend in the MRB. The evapotranspiration in the MRB suggests that the cultivated land is becoming more humid while the other regions are becoming more arid. Long-term change in the land use is mainly promoted due to the multiple years’ efforts on development of the water-saving technologies. This study greatly improves our understanding of the interactions between change in ecological environments and human activities and may provide policy makers guidance of sustainable development at an arid area.
Skin immunization by microneedle patch overcomes statin-induced suppression of immune responses to influenza vaccine
Authors: Vassilieva Elena V. , Wang Shelly , Li Song , Prausnitz Mark R. , Compans Richard W. 
Published: December 2017

AbstractRecent studies indicated that in elderly individuals, statin therapy is associated with a reduced response to influenza vaccination. The present study was designed to determine effects on the immune response to influenza vaccination induced by statin administration in a mouse model, and investigate potential approaches to improve the outcome of vaccination on the background of statin therapy. We fed middle aged BALB/c mice a high fat “western” diet (WD) alone or supplemented with atorvastatin (AT) for 14 weeks, and control mice were fed with the regular rodent diet. Mice were immunized with a single dose of subunit A/Brisbane/59/07 (H1N1) vaccine, either systemically or with dissolving microneedle patches (MNPs). We observed that a greater age-dependent decline in the hemagglutinin inhibition titers occurred in systemically-immunized mice than in MNP- immunized mice. AT dampened the antibody response in the animals vaccinated by either route of vaccine delivery. However, the MNP-vaccinated AT-treated animals had ~20 times higher total antibody levels to the influenza vaccine than the systemically vaccinated group one month postvaccination. We propose that microneedle vaccination against influenza provides an approach to ameliorate the immunosuppressive effect of statin therapy observed with systemic immunization.
The role of the strain induced population imbalance in Valley polarization of graphene: Berry curvature perspective
Authors: Farajollahpour Tohid , Phirouznia Arash 
Published: December 2017

AbstractReal magnetic and lattice deformation gauge fields have been investigated in honeycomb lattice of graphene. The coexistence of these two gauges will induce a gap difference between two valley points (K and K′) of system. This gap difference allows us to study the possible topological valley Hall current and valley polarization in the graphene sheet. In the absence of magnetic field, the strain alone could not generate a valley polarization when the Fermi energy coincides exactly with the Dirac points. Since in this case there is not any imbalance between the population of the valley points. In other words each of these gauges alone could not induce any topological valley-polarized current in the system at zero Fermi energy. Meanwhile at non-zero Fermi energies population imbalance can be generated as a result of the external strain even at zero magnetic field. In the context of Berry curvature within the linear response regime the valley polarization (both magnetic free polarization, Π0, and field dependent response function, χ
α
) in different values of gauge fields of lattice deformation has been obtained.
Strong stability of linear parabolic time-optimal control problems
Authors: Bonifacius Lucas , Pieper Konstantin 
Published: December 2017

Sufficient conditions for strong stability of a class of linear time-optimal control problems with general convex terminal set are derived. Strong stability in turn guarantees qualified optimality conditions. The theory is based on a characterization of weak invariance of the target set under the controlled equation. An appropriate strengthening of the resulting Hamiltonian condition ensures strong stability and yieldsa prioribounds on the size of multipliers, independent of,e.g., the initial point or the running cost. In particular, the results are applied to the control of the heat equation into anL2-ball around a desired state.
Effect of Population-Specific Birthweight Curves on Disparities in Perinatal Mortality in Small-for-Gestational Age Pregnancies
Authors: Naimi Ashley , Larkin Jacob 
Published: December 2017

Objective To determine the effect of adopting sex or race/ethnicity-specific birthweight curves on small-for-gestational age (SGA)-associated mortality rates for specific populations.
Materials and Methods Analyzing 20,095,735 singleton pregnancies, we compared rates of perinatal death associated with SGA in distinct sex and racial/ethnic groups when SGA was defined using nonspecific, sex-specific, and race/ethnicity-specific birthweight curves.
Results With use of a nonspecific birthweight curve, the rate of perinatal death was higher for SGA males (20.4/1,000 [95% confidence interval (CI), 20.1, 20.7]) than SGA females [14.6/1,000 (95% CI, 14.4, 14.8)]. With a sex-specific curve, this disparity was reduced, measuring 17.7/1,000 (95% CI, 17.4, 17.9) for SGA males and 16.3/1,000 (95% CI, 16.1, 16.6) for females. Using a nonspecific birthweight curve, perinatal death rates were higher for non-Hispanic blacks (20.4/1,000 [95% CI, 20.0, 20.8]) than for all other racial/ethnic groups (15.9/1,000 [95% CI, 15.7, 16.1]). This difference increased with use of a race-specific birthweight curve: perinatal mortality was 29.7/1,000 (95% CI, 29.0, 30.3) for SGA blacks and 14.7/1,000 (95% CI, 14.6, 14.9) for all other racial/ethnic groups.
Conclusion Population-based differences in SGA-associated mortality are reduced with adoption of a sex-specific birthweight curve, but widen with use of a race/ethnicity-specific curve. These findings highlight the importance of outcomes analysis in the selection of diagnostic criteria for SGA.
Development of a Clinical Risk Assessment Tool for 6-Week Postpartum Visit Nonadherence
Authors: Kaur Sangeeta , Bahado-Singh Ray , Qafiti Fred 
Published: December 2017

Objective The 6-week postpartum visit (6WPP) is integral in addressing postpartum medical concerns. Failure to attend this routine visit is a measure of suboptimal care. This study aims to identify patients at risk of 6WPP nonadherence by developing a novel point-based risk scoring system.
Methods In this retrospective case–control study (n = 587), a randomly selected subgroup, that is, the “test” group (n = 303), was used to develop the model. The remaining patients were used as an independent “validation” group (n = 284) to assess the model performance.
Results Five factors were found to correlate with 6WPP nonadherence. Positive correlations include: Medicaid health insurance (odds ratio [OR]: 2.40, 95% confidence interval [CI]: 1.38–4.15); prenatal care initiated at ≥ 14 weeks' gestation (OR: 1.82, 95% CI: 1.11–2.96); and maternal age < 24.0 years (OR: 2.02, 95% CI: 1.13–3.61). Factors negatively correlated with nonadherence include: “married” marital status (OR: 0.50, 95% CI: 0.30–0.84) and primiparity (OR: 0.51, 95% CI: 0.30–0.85). The final scoring system demonstrates significant predictive power in both the test and validation groups (respectively, area under the curve = 0.682, p < 0.001 and 0.629, p < 0.001).
Conclusion This risk assessment tool relies on routinely collected data, making its implementation simple. Applying it in the clinical setting allows for early, targeted intervention aimed at minimizing 6WPP nonadherence.