bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide association study of social relationship satisfaction: significant loci and correlations with psychiatric conditions
Authors: Warrier Varun ,   the 23andMe Research Team, Bourgeron Thomas , Baron-Cohen Simon 
Published: September 2017

AbstractDissatisfaction in social relationships is reported widely across many psychiatric conditions. We investigated the genetic architecture of family relationship satisfaction and friendship satisfaction in the UK Biobank. We leveraged the high genetic correlation between the two phenotypes (rg= 0.87±0.03; P < 2.2x10-16) to conduct multi-trait analysis of Genome Wide Association Study (GWAS) (Neffectivefamily = 164,112; Neffectivefriendship = 158,116). We identified two genome-wide significant associations for both the phenotypes: rs1483617 on chromosome 3 and rs2189373 on chromosome 6, a region previously implicated in schizophrenia. eQTL and chromosome conformation capture in neural tissues prioritizes several genes includingNLGN1. Gene-based association studies identified several significant genes, with highest expression in brain tissues. Genetic correlation analysis identified significant negative correlations for multiple psychiatric conditions including highly significant negative correlation with cross-psychiatric disorder GWAS, underscoring the central role of social relationship dissatisfaction in psychiatric diagnosis. The two phenotypes were enriched for genes that are loss of function intolerant. Both phenotypes had modest, significant additive SNP heritability of approximately 6%. Our results underscore the central role of social relationship satisfaction in mental health and identify genes and tissues associated with it.
Functional analyses of the CIF1-CIF2 complex inTrypanosoma bruceiidentify the structural motifs required for complex formation and cytokinesis
Authors: Hu Huiqing , Majneri Paul , Li Dielan , Kurasawa Yasuhiro , An Tai , Dong Gang , Li Ziyin 
Published: September 2017

ABSTRACTCytokinesis in trypanosome occurs uni-directionally along the longitudinal axis from the cell anterior towards the cell posterior and requires a trypanosome-specific CIF1-CIF2 protein complex. However, little is known about the contribution of the structural motifs in CIF1 and CIF2 to complex assembly and cytokinesis. Here, we demonstrated that the two zinc-finger motifs but not the coiled-coil motif in CIF1 are required for interaction with the EF-hand motifs in CIF2. We further showed that localization of CIF1 depends on the coiled-coil motif and the first zinc-finger motif and that localization of CIF2 depends on the EF-hand motifs. Deletion of the coiled-coil motif and mutation of either zinc-finger motifs in CIF1 disrupted cytokinesis. Further, mutation of either zinc-finger motif in CIF1 mis-localized CIF2 to the cytosol and destabilized CIF2, whereas deletion of the coiled-coil motif in CIF1 spread CIF2 over to the new flagellum attachment zone and stabilized CIF2. Together, these results uncovered the requirement of the coiled-coil motif and zinc-finger motifs for CIF1 function in cytokinesis and for CIF2 localization and stability, providing structural insights into the functional interplay between the two cytokinesis regulators.
Mechanisms underlying serotonergic excitation of callosal projection neurons
Authors: Stephens Emily K. , Baker Arielle L. , Gulledge Allan T. 
Published: October 2017

AbstractSerotonin (5-HT) selectively excites subpopulations of pyramidal neurons in the neocortex via activation of 5-HT2A(2A) receptors coupled to Gqsubtype G-protein alpha subunits. Gq-mediated excitatory responses have been attributed primarily to suppression of potassium conductances, including those mediated by KV7 potassium channels (i.e., the M-current), or activation of nonspecific cation conductances that underly calcium-dependent afterdepolarizations (ADPs). However, 2A-dependent excitation of cortical neurons has not been extensively studied, and no consensus exists regarding the underlying ionic effector(s) involved. We tested potential mechanisms of serotonergic excitation in commissural/callosal projection neurons (COM neurons) in layer 5 of the mouse medial prefrontal cortex, a subpopulation of cortical pyramidal neurons that exhibit 2A-dependent excitation in response to 5-HT. In baseline conditions, 5-HT enhanced the rate of action potential generation in COM neurons experiencing suprathreshold somatic current injection. This serotonergic excitation was occluded by activation of muscarinic acetylcholine (ACh) receptors, confirming that 5-HT acts via the same Gq-signaling cascades engaged by ACh. Like ACh, 5-HT promoted the generation of calcium-dependent ADPs following spike trains. However, calcium was not necessary for serotonergic excitation, as responses to 5-HT were enhanced (by >100%), rather than reduced, by chelation of intracellular calcium with 10 mM BAPTA. This suggests intracellular calcium negatively regulates additional ionic conductances contributing to 2A excitation. Removal of extracellular calcium had no effect when intracellular calcium signaling was intact, but suppressed 5-HT response amplitudes, by about 50% (i.e., back to normal baseline values) when BAPTA was included in patch pipettes. This suggests that 2A excitation involves activation of a nonspecific cation conductance that is both calcium-sensitive and calcium-permeable. M-current suppression was found to be a third ionic effector, as blockade of KV7 channels with XE991 (10 μM) reduced serotonergic excitation by ∼50% in control conditions, and by ∼30% with intracellular BAPTA present. These findings demonstrate a role for at least three distinct ionic effectors, including KV7 channels, a calcium-sensitive and calcium-permeable nonspecific cation conductance, and the calcium-dependent ADP conductance, in mediating serotonergic excitation of COM neurons.
Revisiting the erythroid-myeloid lineage decision – a data-driven dynamical model analysis
Authors: Teles Jose , Olariu Victor , Peterson Carsten 
Published: October 2017

AbstractIt is widely conjectured that the mutually antagonizing pair of transcription factors GATA1 and PU.1, deter-mines the choice between the erythroid and myeloid lineages in hematopoiesis. In theoretical approaches, this appears natural with a bistable switch driving the decision. Recent extensive binding and gene expression experiments with some focus on the triad GATA1, GATA2 and PU.1 indicate that GATA2 may be more involved in this lineage decision than previously anticipated. Here, we analyze these experimental data by modeling regulatory sub-networks with deterministic rate equations. Using network dynamical parameters determined by the data, we deduce from increasing the self-interaction bindingsin silicoamong the triad genes that GATA2 and PU.1 exhibit non-linear behavior with one unstable and one stable state. This is in contrast to GATA1, which shows smoother behavior. We extend the network to include the downstream regulators FOG1 and CEBPA, and extract the nature of the corresponding regulatory interactions, excitatory or suppressing, between this pair and the triad by fitting to experimental gene expression time series. Based on this extended network, we simulate and explore different knockout scenarios, providing insight into the role of these regulators in the process of lineage specification, as well as predictions for future experimental validation. We address the mechanism of GATA switching as a mechanism of lineage differentiation by investigating the dynamics of FOG1 regulation by GATA2 and GATA1. Overall, this analysis strongly suggests that within this network, GATA2 is the key driver of erythroid lineage specification through its repression of PU.1, whereas GATA1 appears to be more relevant for the downstream differentiation events.
Knowledge creation and play
Authors: Suorsa Anna Reetta 
Published: October 2017

Purpose
The purpose of this paper is to present a framework for empirically studying knowledge creation (KC) with phenomenological approach and propose that understanding interaction as play conceptualized by Hans-Georg Gadamer allows examining KC starting from the idea of a human being interacting in the events of co-creation. The presented framework is used to examine KC in a community of librarians and teachers collaborating to promote children’s joy of reading.

Design/methodology/approach
An ethnographic approach is applied to investigate knowledge-creating interaction in a working community. The triangulated data consist of ethnographic observations and video recordings of the community’s gatherings, its members’ interviews and produced documents.

Findings
The phenomenological conceptions of temporality of a human being and play are suitable for understanding being in the knowledge-creating interaction, as they give means to understand the meaningfulness of the past experiences, but promote an open attitude toward the future possibilities in a way which promotes KC. Studying interactive events allows understanding how KC can be examined as a collective accomplishment. The playful mode of being in the event was seen as a way to use the limited time available for interaction effectively.

Research limitations/implications
The empirical study was conducted in one community, and further research is needed to test the developed approach in other contexts.

Practical implications
The results may be utilized to develop organizational circumstances, which promote KC by acknowledging the meaningfulness of interaction.

Originality/value
The study presents a novel way to conceptualize and examine KC as an experience and an event with phenomenological approach.
The impact of other customer perception on consumer-brand relationships
Authors: S. Sreejesh , Sarkar Juhi Gahlot , Sarkar Abhigyan , Eshghi Abdolreza , M.R. Anusree 
Published: October 2017

Purpose
Extant research evidence demonstrates that customer satisfaction in a service encounter is influenced by other customer perception (OCP). However, scholarly research on the impact of OCP on brand love and the moderating influence of customers’ attachment styles in the context of hospitality industry is scarce. The purpose of this paper is to fill this gap in the literature.

Design/methodology/approach
The data were collected from customers of five and three-star hotels in India. Regression-based process analysis (Hayes, 2013) was used to test the proposed set of hypotheses.

Findings
The data analysis shows that OCP predicts brand love through the mediation of satisfaction. Individual’s anxious attachment style positively moderates “other customer perception-satisfaction” relationship, and avoidant attachment style negatively moderates the same relationship. Thus the effect of OCP is positively moderated by anxious attachment style, and negatively moderated by avoidant attachment style.

Originality/value
The value of this study lies in quantitatively investigating the roles played by OCP and individual attachment styles in shaping brand love in hospitality industry. In contrast to findings from previous research, the findings from this study suggest that anxious attachment style positively influences brand satisfaction and formation of brand love.
Involvement of the JAK‐STAT pathway in collagen regulation of decidual NK cells
Authors: Fu Qiang , Sun Yufei , Tao Yu , Piao Hailan , Wang Xiaoqiu , Luan Xiying , Du Meirong , Li Dajin 
Published: October 2017

ProblemThe mechanisms underlying the regulation of decidual natural killer cells (dNKs) at the maternal‐fetal interface are unclear.Method of studyPrimary trophoblasts (TROs), decidual stromal cells (DSCs), and dNKs were cocultured, and responses to LAIR‐2 (LAIR‐1 inhibitor) and P4H shRNA (collagen inhibitor) were studied.ResultsCoculture of dNKs with primary TROs/DSCs resulted in downregulation of Th1 cytokine production by dNKs. These effects were abrogated by LAIR‐2 and P4H shRNA. LAIR‐1 binds to SHP‐1, which in turn binds to JAK1 and JAK2. Further, the phosphorylation of STAT1/STAT4 and the expression of the downstream transcription factors T‐bet and Helios in dNKs were decreased by collagen treatment and primary TROs/DSCs coculture.ConclusionThe JAK‐STAT pathway and its downstream transcription factors T‐bet and Helios are involved in the regulation of dNK function by collagen/LAIR‐1 interaction, and this signaling mechanism may contribute to the maintenance of immune tolerance at the maternal‐fetal interface.
Improved neonatal outcome after active management of prolonged pregnancies beyond 41+2 weeks in nulliparous, but not among multiparous women
Authors: Lindegren Lina , Stuart Andrea , Herbst Andreas , Källén Karin 
Published: September 2017

AbstractIntroductionProlonged pregnancies are associated with adverse maternal and fetal outcome. In Sweden, no national guidelines exist for when to induce prolonged pregnancies.Material and methodsSingleton cephalic prolonged pregnancies (defined as ≥ 41+3 gestational weeks) during 2001–2013 (n = 199 770) were identified using the Swedish Medical Birth Register. The maternity units were divided into three groups (tertiles) based on the proportion of pregnancies ≥ 42+3 gestational weeks among all pregnancies ≥ 41+3 weeks. The pregnancy outcome among women delivered at the units with the most expectant management of prolonged pregnancies (> 17.6% proceeding to 42+3 weeks), was compared with that among women delivered at units with the most active management (< 12.6% proceeding to 42+3 weeks). Odds ratios (OR) were calculated using simple and multiple logistic regression. Adjustments were made for year of delivery, hospital level, maternal age, body mass index, and smoking.ResultsAmong primiparas, an increased risk of Apgar score < 7 at 5 minutes [odds ratio (OR) 1.27, 95% CI 1.16–1.41] and meconium aspiration (OR 1.49, 95% CI 1.14–1.95) was found after birth at most expectant units compared with most active units, but among multiparas, no such associations were detected. A decreased rate of cesarean section was found for both primiparas (OR 0.83, 95% CI 0.80–0.86) and multiparas (OR 0.82, 95% CI 0.77–0.86) at units with expectant vs. active management. No association between perinatal death and delivery‐unit specific management of prolonged pregnancies was detected.ConclusionsOffspring to primiparas might gain from a more active management of prolonged pregnancies, whereas no such improvement of neonatal outcome among multiparous women was detected.
Diagnostic accuracy of ultrasound in detecting the severity of abnormally invasive placentation: a systematic review and meta‐analysis
Authors: Pagani Giorgio , Cali Giuseppe , Acharya Ganesh , Trisch Ilan‐Timor , Palacios‐Jaraquemada Jose , Familiari Alessandra , Buca Danilo , Manzoli Lamberto , Flacco Maria E. , Fanfani Francesco , Liberati Marco , Scambia Giovanni , D'antonio Francesco 
Published: September 2017

AbstractIntroductionOur objective was to elucidate the overall diagnostic accuracy of ultrasound in detecting the severity of abnormally invasive placentation (AIP).Material and methodsMedline, Embase, CINAHL and The Cochrane databases were searched. The ultrasound signs explored were: loss of hypoechoic (clear) zone in the placental–uterine interface, placental lacunae, bladder wall interruption, myometrial thinning, focal exophitic mass, placental lacunar flow, subplacental vascularity, and uterovesical hypervascularity.ResultsTwenty studies (3209 pregnancies) were included. Ultrasound had an overall good diagnostic accuracy in identifying the depth of placental invasion with sensitivities of 90.6%, 93.0%, 89.5%, and 81.2% for placenta accreta, increta, accreta/increta, and percreta, respectively; the corresponding specificities were 97.1%, 98.4%, 94.7%, and 98.9%. Placental lacunae had sensitivities of 74.8%, 88.6%, and 76.3% for the detection of placenta accreta, increta, and percreta, respectively. Sensitivity and specificity of loss of the clear zone in identifying placenta accreta were 74.9% and 92.0%, whereas the corresponding figures for placenta increta and percreta were 91.6% and 76.9%, and 88.1% and 71.1%. Lacunar flow had sensitivities of 81.2%, 84.3%, and 45.2% for the detection of placenta accreta, increta, and percreta respectively; the corresponding figures for specificity were 84.0%, 79.7%, and 75.3%. Sensitivity of uterovesical hypervascularity was low for the detection of placenta accreta (12.3%) but high for placenta increta (94.4%) and percreta (86.2%); the corresponding figures for specificity were 90.8%, 88.0% and 88.2%, respectively.ConclusionsUltrasound has an overall good diagnostic accuracy in recognizing the depth and the topography of placental invasion.
Development of one paediatric and one neonatal formulary list in hospital settings
Authors: Haslund‐Krog Sissel Sundell , Rolighed Christensen Hanne , Bjerager Mia , Holst Helle 
Published: September 2017

AimsThe aim of this study is to describe the stepwise process towards creating two formulary lists: one for paediatric and one for neonatal patients covering common diseases in hospital settings.MethodsThis study presents the concept for developing a formulary list, namely how to: (1) organize the editorial board, (2) procure drug consumption data and database management, including information on labelling status, dosing options, excipients and problematic adverse events, current guidelines, evidence and price, (3) develop the first edition for the formulary list and formulary manual, and (4) to establish a paediatric sub‐committee within the Regional Drug and Therapeutic Committee to maintain and continually develop the two formularies.ResultsThe total number of drugs was 411 ATC level 5, which covers 1097 unique item numbers prior to the paediatric formulary list, of which 263 item numbers were included in the final list. In neonates, 201 drugs ATC level 5 were evaluated, covering 348 unique item numbers, of which 104 item numbers were included in the final neonatal formulary list. Eighty‐eight percent of the included drugs in the paediatric formulary were licensed to children (not specified by age group), 2% were unlicensed in Denmark, and 7% were extemporaneous preparations. For neonates, the percentage was 48%, 4% and 16%, correspondingly.ConclusionThe process is time‐consuming as studies are lacking and age‐appropriate dosage forms and concentrations differ amongst countries. Nevertheless, the process should be somewhat similar between countries, albeit different drugs may be selected for the final formulary lists.
Developmental cascade models linking peer victimization, depression, and academic achievement in Chinese children
Authors: Liu Junsheng , Bullock Amanda , Coplan Robert J. , Chen Xinyin , Li Dan , Zhou Ying 
Published: October 2017

This study explored the longitudinal relations among peer victimization, depression, and academic achievement in Chinese primary school students. Participants were N = 945 fourth‐grade students (485 boys, 460 girls; Mage = 10.16 years, SD = 2 months) attending elementary schools in Shanghai, People's Republic of China. Three waves of data on peer victimization, depression, and academic achievement were collected from peer nominations, self‐reports, and school records, respectively. The results indicated that peer victimization had both direct and indirect effects on later depression and academic achievement. Depression also had both direct and indirect negative effects on later academic achievement, but demonstrated only an indirect effect on later peer victimization. Finally, academic achievement had both direct and indirect negative effects on later peer victimization and depression. The findings show that there are cross‐cultural similarities and differences in the various transactions that exist among peer victimization, depression, and academic achievement.
Statement of contribution

What is already known on this subject?

Peer victimization directly and indirectly relates to depression and academic achievement.

Depression directly and indirectly relates to academic achievement.

Academic achievement directly and indirectly relates to depression.

What the present study adds?

A developmental cascade approach was used to assess the interrelations among peer victimization, depression, and academic achievement.

Academic achievement mediates the relation between peer victimization and depression.

Depression is related to peer victimization through academic achievement.

Academic achievement directly and indirectly relates to peer victimization.

Academic achievement is related to depression through peer victimization.
Authoring and enactment of mobile pyramid‐based collaborative learning activities
Authors: Manathunga Kalpani , Hernández‐Leo Davinia 
Published: October 2017

AbstractCollaborative learning flow patterns (CLFPs) formulate best practices for the orchestration of activity sequences and collaboration mechanisms that can elicit fruitful social interactions. Mobile technology features offer opportunities to support interaction mediation and content accessibility. However, existing mobile collaborative learning research has mostly focussed on simple activity orchestrations from the perspective of collaborative flow orchestration and flexibility requirements, predominantly in face‐to‐face pre‐university educational contexts. This paper proposes a particularisation of the Pyramid CLFP to support flexible face‐to‐face and distance mobile learning scenarios in which learners interact in increasingly larger groups along a sequence of activities (Pyramid levels). PyramidApp implements this Pyramid particularisation that provides both a web‐based authoring tool and an enactment tool accessible through web or mobile devices. The authoring tool was evaluated in workshops where teachers appreciated its design and applicability to their educational contexts. PyramidApp flows were enacted in three higher education settings. Learners enjoyed the activities but usage and satisfaction varied depending on several design and contextual factors like the epistemic tasks given, the education level and application mode (face‐to‐face or distance).
BIO 300, a nanosuspension of genistein, mitigates pneumonitis/fibrosis following high‐dose radiation exposure in the C57L/J murine model
Authors: Jackson Isabel L , Zodda Andrew , Gurung Ganga , Pavlovic Radmila , Kaytor Michael D , Kuskowski Michael A , Vujaskovic Zeljko 
Published: September 2017

Background and PurposeBIO 300 nanosuspension (Humanetics Corporation) is being developed as a medical countermeasure (MCM) for the mitigation of the delayed effects of acute radiation exposure, specifically pneumonitis and fibrosis of the lung. The objective of this study was to determine the best dose and treatment duration of BIO 300 to mitigate lung injury and improve the likelihood for survival in C57L/J mice exposed to whole thorax lung irradiation (WTLI).Experimental ApproachAge‐ and sex‐matched C57L/J mice received a single dose of 11.0 or 12.5 Gy WTLI. BIO 300 (200 or 400 mg·kg−1, oral gavage) was administered daily starting 24 h post‐exposure for a duration of 2, 4, 6 or, in some cases, 10 weeks. Non‐treated controls were included for comparison in both sexes. Animals were observed daily for signs of major morbidity. Respiratory function was assessed biweekly. Lungs were collected, weighed and paraffin embedded for histological evaluation post mortem.Key ResultsBIO 300 administered at an oral dose of 400 mg·kg−1 for 4 to 6 weeks starting 24 h post‐WTLI reduced morbidity associated with WTLI. The improvement in survival correlated with reduced respiratory frequency and enhanced pause. The irradiated lungs of mice treated with BIO 300 (400 mg·kg−1) for 4 to 6 weeks displayed less morphological damage and airway loss due to oedema, congestion and fibrotic scarring than the untreated, irradiated controls.Conclusions and ImplicationsBIO 300 is a promising MCM candidate to mitigate pneumonitis/fibrosis when administered daily for 4–6 weeks starting 24 h post‐exposure.