bluuebunny/crossref_metadata_2025_split huggingface dataset:
Girdin (GIV) Expression as a Prognostic Marker of Recurrence in Mismatch Repair–Proficient Stage II Colon Cancer
Authors: Ghosh Pradipta , Tie Jeanne , Muranyi Andrea , Singh Shalini , Brunhoeber Patrick , Leith Katherine , Bowermaster Rebecca , Liao Zhiming , Zhu Yifei , LaFleur Bonnie , Tran Ben , Desai Jayesh , Jones Ian , Croxford Matthew , Jover Rodrigo , Goel Ajay , Waring Paul , Hu Song , Teichgraber Volker , Rohr Ulrich-Peter , Ridder Ruediger , Shanmugam Kandavel , Gibbs Peter 
Published: March 2016

Abstract
Purpose: Prognostic markers that identify patients with stage II colon cancers who are at the risk of recurrence are essential to personalize therapy. We evaluated the potential of GIV/Girdin as a predictor of recurrence risk in such patients.
Experimental Design: Expression of full-length GIV was evaluated by IHC using a newly developed mAb together with a mismatch repair (MMR)-specific antibody panel in three stage II colon cancer patient cohorts, that is, a training (n = 192), test (n = 317), and validation (n = 181) cohort, with clinical follow-up data. Recurrence risk stratification models were established in the training cohort of T3, proficient MMR (pMMR) patients without chemotherapy and subsequently validated.
Results: For T3 pMMR tumors, GIV expression and the presence of lymphovascular invasion (LVI) were the only factors predicting recurrence in both training (GIV: HR, 2.78, P = 0.013; LVI: HR, 2.54, P = 0.025) and combined test and validation (pooled) cohorts (GIV: HR, 1.85, P = 0.019; LVI: HR, 2.52, P = 0.0004). A risk model based on GIV expression and LVI status classified patients into high- or low-risk groups; 3-year recurrence-free survival was significantly lower in the high-risk versus low-risk group across all cohorts [Training: 52.3% vs. 84.8%; HR, 3.74, 95% confidence interval (CI), 1.50–9.32; Test: 85.9% vs. 97.9%, HR, 7.83, 95% CI, 1.03–59.54; validation: 59.4% vs. 84.4%, HR, 3.71, 95% CI, 1.24–11.12].
Conclusions: GIV expression status predicts recurrence risk in patients with T3 pMMR stage II colon cancer. A risk model combining GIV expression and LVI status information further enhances prediction of recurrence. Further validation studies are warranted before GIV status can be routinely included in patient management algorithms. Clin Cancer Res; 22(14); 3488–98. ©2016 AACR.
Lysyl Oxidase (LOX) Transcriptionally Regulates SNAI2 Expression and TIMP4 Secretion in Human Cancers
Authors: Boufraqech Myriem , Zhang Lisa , Nilubol Naris , Sadowski Samira M. , Kotian Shweta , Quezado Martha , Kebebew Electron 
Published: March 2016

Abstract
Purpose: Epithelial-to-mesenchymal transition (EMT) is important in cancer progression and metastasis. We and others have previously reported that lysyl oxidase (LOX) is overexpressed in aggressive cancers, is associated with increased mortality, and regulates EMT. However, the mechanism by which LOX mediates EMT is unknown. In this study, we investigated the effect of LOX on mediators of EMT.
Experimental Design: We used chromatin immunoprecipitation and promoter luciferase assays to determine the target gene of LOX. To determine the effects of SNAI2 in vivo, we used our metastatic anaplastic thyroid cancer (ATC) mouse model. To investigate the effects of LOX and SNAI2 on MMPs and TIMPs, protein arrays were used. Primary tumors from patients with metastatic, breast and colon cancer, and tissue array for thyroid cancer were assessed for SNAI2 and TIMP4 expression by immunohistochemistry.
Results: We found that LOX knockdown decreases SNAI2 expression in cancer cell lines. Furthermore, knockdown of LOX reduced SNAI2 expression in a metastatic mouse model of thyroid cancer. We also demonstrated that LOX binds and transactivates the SNAI2 promoter. We found a direct correlation in thyroid and breast cancer samples between LOX and SNAI2 expression. To understand how LOX/SNAI2 axis mediates these effects, we performed a comprehensive analysis of MMPs/TIMPs. LOX and SNAI2 depletion reduced TIMP4 secretion. Analysis of SNAI2 and TIMP4 expression showed overexpression of both proteins in aggressive thyroid, colon, and breast tumors.
Conclusions: Our findings provide new evidence that LOX regulates SNAI2 expression and that SNAI2-mediated TIMP4 secretion plays a role in cancer progression. Clin Cancer Res; 22(17); 4491–504. ©2016 AACR.
Statin and Aspirin Use Among Hispanic and Latino Adults at High Cardiovascular Risk: Findings From the Hispanic Community Health Study/Study of Latinos
Authors: Qato Dima M. , Lee Todd A. , Durazo‐Arvizu Ramon , Wu Donghong , Wilder Jocelyn , Reina Samantha A. , Cai Jianwen , Gonzalez Franklyn , Talavera Gregory A. , Ostfeld Robert J. , Daviglus Martha L. 
Published: March 2016

Background

Despite variations in the prevalence of cardiovascular disease and related risk factors among
US
Hispanic/Latino adults of diverse backgrounds, there is little information on whether disparities exist in the use of medications for the primary and secondary prevention of cardiovascular disease. We examined the prevalence of statin and aspirin use among diverse
US
Hispanic/Latino adults at high cardiovascular risk.

Methods and Results

A multicenter population‐based study, the Hispanic Community Health Study/Study Of Latinos, included a total of 16 415 participants of Mexican, Puerto Rican, Cuban, Dominican, South American, and Central American backgrounds who were aged 18 to 74 years and enrolled between March 2008 and June 2011. Our analyses were limited to 4139 participants considered to be at high cardiovascular risk. Age‐adjusted prevalence of statin and aspirin use was 25% and 44%, respectively, overall but varied by Hispanic/Latino background among those at high cardiovascular risk; statin use was significantly higher (
P
<0.001) among adults of Puerto Rican (33%) and Dominican (28%) backgrounds compared with adults of other backgrounds (Mexican, 24%; Cuban, 22%; Central American, 20%; South American, 22%). There was no difference in aspirin use. After adjusting for health insurance coverage, the difference in prevalence of statin use was substantially reduced among participants with a Puerto Rican background, from an odds ratio of 1.73 (95%
CI
1.30–2.31) to 1.30 (95%
CI
0.97–1.75), and with a Dominican background, from an odds ratio of 1.45 (95%
CI
1.04–2.02) to 1.07 (95%
CI
0.75–1.52), in comparison to their counterparts.

Conclusions

Among Hispanic/Latino adults of diverse backgrounds, statin use was more prevalent among adults with Puerto Rican and Dominican backgrounds at high cardiovascular risk. These differences in statin use were explained, in part, by differences in insurance coverage. These findings have important implications for the prevention of disparities in cardiovascular outcomes within the growing
US
Hispanic/Latino population.
Upwelling of Atlantic Water along the Canadian Beaufort Sea Continental Slope: Favorable Atmospheric Conditions and Seasonal and Interannual Variations
Authors: Kirillov Sergei , Dmitrenko Igor , Tremblay Bruno , Gratton Yves , Barber David , Rysgaard Søren 
Published: March 2016

Abstract
The role of wind forcing on the vertical displacement of the −1°C isotherm and 33.8 isohaline depths was examined based on snapshots of historical (1950–2013) temperature and salinity profiles along the Mackenzie continental slope (Beaufort Sea). It is found that upwelling is correlated with along-slope northeast (T59°) winds during both ice-free and ice-covered conditions, although the wind impact is more efficient during the ice-free season. One of the most important factors responsible for vertical displacements of isopycnals is sustained wind forcing that can last for several weeks and even longer. It accounts for 14%–55% of total variance in isotherm/isohaline depths, although these numbers might be underestimated. The upwelling and downwelling events are discussed in the context of the interplay between two regional centers of action—the Beaufort high and Aleutian low—that control the wind pattern over the southern Beaufort Sea. The probability of upwelling-favorable wind occurrence is closely related to the sea level pressure difference between these two centers, as well as their geographical positions. The combined effect of both centers expressed as the SLP differences is highly correlated (0.68/0.66 for summer/winter) with occurrences of extreme upwelling-favorable northeast (NE) winds over the Mackenzie slope, although the Beaufort high plays a more important role. The authors also diagnosed the predominant upwelling-favorable conditions over the Mackenzie slope in the recent decade associated with the summertime amplification of the Beaufort high. The upwelling-favorable NE wind occurrences also demonstrate the significant but low (−0.30) correlation with Arctic Oscillation (AO) during both summer and winter seasons, whereas the high correlation with North Pacific index (NPI; −0.52) is obtained only for the ice-covered period.
Plantaris Excision and Ventral Paratendinous Scraping for Achilles Tendinopathy in an Athletic Population
Authors: Bedi Harvinder S. , Jowett Charlie , Ristanis Stavros , Docking Sean , Cook Jill 
Published: March 2016

Background: Achilles tendinopathy is a frequent problem in high-level athletes. Recent research has proposed a combined etiologic role for the plantaris tendon and neovascularization. Both pathologies can be observed on ultrasound imaging.1,13 However, little is known about the change in structure of the Achilles tendon following the surgical treatment of these issues. The purpose of the study was to assess if excising the plantaris and performing ventral paratendinous “scraping” of the neovascularization improved symptoms of Achilles tendinopathy and whether there was a change in the fibrillar structure of the tendon with ultrasound tissue characterization (UTC) following this operation. Methods: This prospective consecutive case series included 15 professional/semiprofessional athletes (17 Achilles tendons) who underwent plantaris excision and paratendinous scraping to treat noninsertional Achilles tendinopathy. The plantaris tendon was excised if adherent to the Achilles tendon, and the area of neovascularization for scraping was demarcated on preoperative imaging. Preoperative and postoperative Victorian Institute of Sports Assessment-Achilles (VISA-A) scores were taken. UTC was performed on 11 of 17 tendons preoperatively and postoperatively. The mean follow-up was for 25 months. Results: Fourteen of 15 patients had a successful outcome after the surgery. The mean VISA-A improved from 51 to 95 (p=.0001). There was a statistically significant (p=.04) improvement in the aligned fibrillar structure of the tendon confirmed with UTC scanning following surgery from 90% (±8) to 96% (±5). Conclusion: This group of high-level athletes derived an excellent clinical result from this operation. Furthermore, UTC scanning offered an objective method to evaluate the healing of Achilles tendons. Level of Evidence: Level IV, case series.
Outcome of Arthroscopic Treatment of Posterior Impingement of the Ankle
Authors: Carreira Dominic S. , Vora Anand Mahesh , Hearne Kelly L. , Kozy John 
Published: March 2016

Background: Open and arthroscopic techniques have been utilized in the treatment of posterior impingement of the ankle and hindfoot. Because posterior impingement occurs more frequently in patients who repetitively plantarflex the ankle, this population may especially benefit from a procedure that reduces pain and results in maximal range of motion (ROM). The purpose of this study was to assess the outcome of hindfoot endoscopy in patients with posterior ankle impingement through a higher level of function outcome measures and physical examination parameters, focused on analysis of ROM. Methods: Twenty patients were followed prospectively at a minimum 1-year follow-up (mean 38.2 months). Nineteen of 20 patients were competitive athletes. Patients completed a minimum of 3 months of nonoperative treatment. Diagnoses included os trigonum, tibial exostosis, talar exostosis, loose body or fracture nonunion, and ganglion cyst removal. Patients underwent arthroscopic treatment utilizing a posterior approach; all relevant pathology was addressed. Results: At the most recent follow-up, visual analog scale pain and American Orthopaedic Foot & Ankle Society hindfoot scores showed significant improvement ( P < .01) pre- to postoperatively; Tegner score remained unchanged ( P = .888). Three patients were professional athletes; all returned to their previous level of professional activity. ROM variables between affected and unaffected sides reached statistical similarity at the most recent follow-up. Only ankle plantarflexion reached statistical significance when compared pre- to postoperatively. Fifteen percent of patients reported postoperative neuritis. Conclusions: Posterior ankle arthroscopy allowed for maintenance or restoration of anatomic ROM of the ankle and hindfoot, ability to return to at least previous level of activity, and improvement in objective assessment of pain relief and higher level of function parameters. Complications associated with this procedure were minimal. Level of Evidence: Level IV, retrospective case series.
Texting Adolescents in Repeat DKA and Their Caregivers
Authors: Wagner David V. , Barry Samantha , Teplitsky Lena , Sheffield Annan , Stoeckel Maggie , Ogden Jimmie D. , Karkula Elizabeth , Hartman Alexandra , Duke Danny C. , Spiro Kim , Harris Michael A. 
Published: March 2016

Background: Text message interventions are feasible, preferable, and sometimes effective for youth with diabetes. However, few, if any studies, have examined the personalized use of text messages with youth repeatedly hospitalized for diabetic ketoacidosis (DKA) and their caregivers. This study characterizes the use of personalized text messages in Novel Interventions in Children’s Healthcare (NICH). Methods: Approximately 2 months of text messages sent to youth with repeat DKA and their caregivers were logged regarding the following text characteristics: (1) content, (2) intervention type, (3) timing, and (4) recipient characteristics. Results: NICH interventionists sent 2.3 and 1.5 texts per day to patients and caregivers, respectively. Approximately 59% of outgoing texts occurred outside of typical business hours, and roughly 68% of texts contained some form of support and/or encouragement. The relation between type of intended intervention and day/time of text was significant, χ2(2, N = 5,808) = 266.93, P < .001. Interventionists were more likely to send behavioral intervention text messages outside of business hours, whereas they were more likely to send care coordination and case management text messages during business hours. Conclusions: To our knowledge, this is the first study to specifically categorize and describe the personalized use of text messages with youth repeatedly hospitalized for DKA and their caregivers. Findings indicate that a promising treatment program for these youth frequently used text interventions to deliver praise and encouragement to patients and caregivers alike, often outside of typical business hours, and tailored text content based on patient and caregiver characteristics.
Peri- and intraarticular analgesia (PIA) superior to other procedures in TKA – review of literature and results of own RCT
Authors: Beckmann J. , Stathellis A. , Fitz W. , Köck F. , Bauer G. , Gebauer M. , Schnurr C. 
Published: March 2016

Background: TKA is a worldwide established surgery with periarticular infiltrations being scientifically funded is the superiority of in the perioperative setup of TKA nowadays. However, nerve blocks are still widely used as perioperative standard meaning possible analgesia beyond surger, but simultaneously with sensomotoric deficiency and resulting risk of fall as well as increased pain after removal of nerval catheters. Also a standard procedure is intubation which means immediate pain after awakening of patients with resulting need for sufficient oral, intravenous, intramuscular or subcutaneous analgesia. We present an actual review of the literature and an own RCT comparing infiltrations with nerve blocks, which to our best knowledge presents the first RCT combined with an additional continous part. Methods: Literature review (PubMed) Own RCT: 50 TKA were randomized and prospectively included. 25 patients received nerve blocks with a single ischiadicus block and a femoralis block with additional perineural catheter. 25 patients received periarticular infiltrations together with an intraarticular catheter (PIA). All catheters stayed for 4 days. Both groups received a laryngeal masc. Postoperative mobilisation, surgeon and type of prosthesis were the same in all patients. The following was evaluated pre- and postoperatively (first, third and sixth hour, first until sixth day): VAS, additional analgetics/ opioids, KSS score, knee function and ability to raise the straightened leg. Complications as infection, falls, DVT etc. were recorded. Results: Periarticular infiltrations are superior to other options in the perioperative setup of TKA which is clearly shown by literature (several studies including reviews) as well as the results of our own RCT: Pain occured in both groups, however, VAS, additional analgetics/ opioids and KSS score as well as the ability to raise the straightened leg were significantly better following PIA (p<0,01), with comparable knee function in both groups. There were two falls and one superficial infection of catheter site following nerve blocks, however, without sequelae. Conclusion: All analgesic/ anaesthetic options can be done perioperatively in TKA with success with, however, clear superiority of PIA. Compared to nerve blocks PIA reduces the risk for falls, prevents a pain-„rebound“ with removal of perineural catheters and is beneficial for patients throughout the whole hospitalisation.
In vivo Investigation of Patellofemoral Cartilage Contact Mechanisms- Impact of Mechanical Loading and Patellofemoral Geometry
Authors: Izadpanah Kaywan , Niemeyer Philip , Meine Hans , Südkamp Norbert P. , Lang Thomas 
Published: March 2016

Aims and Objectives: Today the selection of the procedures for treatment of focal cartilage lesions is standardized to the defect size. Subject specific parameters like individual cartilage contact mechanisms are not taken into account. Aim of the present study therefore was to investigate inter-individual differences in the patellofemoral cartilage contact mechanisms of healthy subjects and to correlate them to the individual patellar joint surface area and patellar and femoral dimensions. Materials and Methods: 10 knees of 10 healthy subjects with regular shaped patella and trochlea underwent magnetic resonance imaging at rest and during mechanical loading for simulated weight bearing. Imaging was performed at 30° knee flexion. A custom made pneumatic loading device was used to apply 0 and 200 N axial loading to the leg. Manual segmentation of the patella, the trochlear and the patellofemoral cartilage was performed. Patellar volume, joint surface area and cartilage contact areas were calculated for each loading condition. Moreover patellar height, diameter and femoral epicondylar width were determined. Prospective motion correction was performed to reduce motion artifacts during load application. Results: Mean size of the patellar surface area was 16,08 cm2 (range 13,11-18,07 cm2). Mean patellofemoral contact area (PCA) without loading was 6,15 cm2 (range 5,12-7,33 cm2). During axial loading of the leg with 200N PCA significantly increased to 6,77 cm2 (range 6,05-7,55 cm2) (p<0,05). Patellar surface area and PCA were not correlated (r=0,01; p<0,05). Mean Patella width was 5,4 cm (Range 4,8-6,2 cm) and mean femoral width at the epicondyles was 8,5 cm (Range 7,9-9 cm). The mean patellofemoral cartilage contact area showed poor correlation to patellar geometry (patellar width (r=0,48, p<0,05; patellar cartilage surface r=0,06, p<0,05) but moderate correlation to the femoral width (r=0,75, p<0,05). Conclusion: Main finding of the presented study is that patellofemoral cartilage contact mechanisms are responsive to loading and show relevant inter individual differences. A second important finding is that cartilage contact mechanisms cannot be concluded directly by geometric parameters such as joint surface area or patellofemoral dimensions. Further investigation is needed to identify parameters influencing size of patellofemoral contact area at rest and during loading in order to include them into diagnostics and treatment of the cartilage defects in clinical routine.
Septic Arthritis Following Arthroscopic Anterior Cruciate Ligament Reconstruction
Authors: Schuster Philipp 
Published: March 2016

Aims and Objectives: To determine the incidence of postoperative septic arthritis following primary and revision ACL-reconstruction, to identify the microbiological spectrum, and to evaluate a graft-retaining treatment protocol consisting of sequential arthroscopic revisions. Materials and Methods: From January 2004 to June 2014 a total of 7,096 consecutive arthroscopic ACL reconstructions were performed at our institution (5,907 primary and 1,189 revision reconstructions). Thereof, cases with postoperative septic arthritis were identified and retrospectively analyzed with regard to incidence, clinical presentation, time to and number of arthroscopic revisions, clinical course and microbiological findings. Follow-up examination consisted of clinical examination, instrumental measurement (KT-1000), classification according to objective IKDC (International Knee Documentation Committee) score, subjective IKDC score and radiologic evaluation. Results: The incidence of septic arthritis was 0.51%, with 0.41% in primary and 1.01% in revision reconstructions, respectively (p = 0.008; odds ratio 2.5). First revision was performed 19.6 ? 10.6 days after index procedure. Eradication was achieved in all cases after an average of 2.25 ? 1.22 arthroscopic revisions, with graft retainment in all but one case (97.2%). Coagulase-negative Staphylococci (62.5%) and Staphylococcus aureus (21.9%) were the most frequent pathogens. Twenty-nine patients were available for follow-up (80.6%) after 4.7 ? 3.2 years (range 0.7 to 11.2). No reinfection was seen. Two patients suffered recurrent non-traumatic ACL insufficiency (6.9%), all others (93.1%) had an intact ACL graft with a KT-1000 side-to-side difference of 1.4 ? 0.9mm. Subjective IKDC score was 80.4 ? 11 points. No emerging or deterioration of osteoarthritis related to the infection was seen. Conclusion: Postoperative septic arthritis is a rare but serious complication after arthroscopic ACL reconstruction. Retention of the graft and good to excellent clinical results can be obtained with an arthroscopic treatment protocol.