bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Function of Emulsions on the Biogenic Amine Formation and their Indices of Sea Bass Fillets (Dicentrarchus Labrax) Stored in Vacuum Packaging
Authors: Ozogul Yesim , Durmus Mustafa , Kuley Boga Esmeray , Uçar Yılmaz , Ozogul Fatih 
Published: January 2018

AbstractThe impacts of emulsions based on commercial oils on the biogenic amine formation and their indices of vacuumed packed sea bass fillets were investigated. The results showed that among biogenic amines, cadaverine, putrescine, spermidine, spermine, serotonin, dopamine, and agmatine were predominant amines in sea bass fillets stored under vacuum packaging. Significant differences (P < 0.05) in biogenic amines concentrations of vacuumed packed sea bass treated with emulsions were observed. All groups contained histamine lower than 5.0 mg/100 g, regarded as the allowable limit by the U.S. Food and Drug Administration. Polyamine levels were not affected by application of emulsion. Quality index (QI) showed an increase and after 14 d of storage it decreased in all groups. The control generally seemed to higher QI value than those of treatment groups except at 14 and 18 days while soybean and corn gave lower QI among treatment groups. Only biogenic amine index correlated with sensory acceptability of vacuumed packed sea bass, indicating that this index can be used for determination of the degree of spoilage of vacuumed packed sea bass. Emulsions extended the shelf‐life (approximately 2 to 4 d) of vacuumed packed sea bass fillets by inhibiting microbial growth compared to the control.Practical ApplicationEmulsions have become popular since they are regarded as ideal carrier for the delivery of lipophilic substances due to the ease of preparation, small particle size, their enhanced bioavailability, and long term kinetic stability. They have been proven to be self‐preserving antimicrobials due to bound water in their structure and thus no available water to microorganisms. Antimicrobial emulsions have potential applications in many fields because they are inexpensive, stable, and nontoxic agents.
Consumer Acceptance Comparison Between Seasoned and Unseasoned Vegetables
Authors: Feng Yiming , Albiol Tapia Marta , Okada Kyle , Castaneda Lazo Nuria Blanca , Chapman‐Novakofski Karen , Phillips Carter , Lee Soo‐Yeun 
Published: January 2018

AbstractRecent findings show that approximately 87% of the U.S. population fail to meet the vegetable intake recommendations, with unpleasant taste of vegetables being listed as the primary reason for this shortfall. In this study, spice and herb seasoning was used to enhance palatability of vegetables, in order to increase consumer acceptance. In total, 749 panelists were screened and recruited as specific vegetable likers of the vegetable being tested or general vegetable likers. Four sessions were designed to evaluate the effect of seasoning within each type of vegetable, including broccoli, cauliflower, carrot, and green bean. Each panelist was only allowed to participate in one test session to evaluate only one vegetable type, so as to mitigate potential learning effect. Overall, the results showed that seasoned vegetables were significantly preferred over unseasoned vegetables (P < 0.001), indicating the sensory properties were significantly improved with seasoning. When general vegetable likers and specific vegetable likers were compared in terms of their preference between seasoned and unseasoned vegetables, the pattern varied across different vegetables; however, general trend of seasoned vegetable being preferred remained. The findings from this study demonstrate the effect of seasoning in enhancing consumer liking of vegetables, which may lead to increased consumption to be assessed in future studies.Practical ApplicationTo improve the sensory properties of vegetables, masking the bitter taste of vegetables using spice and herb seasoning are gaining increasing attention. Our findings suggest that the overall liking of vegetables could be improved by incorporating spice and herb seasonings that are specifically formulated for each vegetable. Ultimately, developing and commercializing spice and herb seasonings may aid to increase vegetable consumption, as well as expanding the vegetable seasoning market.
Selection of Yeast Strains for Tequila Fermentation Based on Growth Dynamics in Combined Fructose and Ethanol Media
Authors: Aldrete‐Tapia J. A. , Miranda‐Castilleja D. E. , Arvizu‐Medrano S. M. , Hernández‐Iturriaga M. 
Published: January 2018

AbstractThe high concentration of fructose in agave juice has been associated with reduced ethanol tolerance of commercial yeasts used for tequila production and low fermentation yields. The selection of autochthonous strains, which are better adapted to agave juice, could improve the process. In this study, a 2‐step selection process of yeasts isolated from spontaneous fermentations for tequila production was carried out based on analysis of the growth dynamics in combined conditions of high fructose and ethanol. First, yeast isolates (605) were screened to identify strains tolerant to high fructose (20%) and to ethanol (10%), yielding 89 isolates able to grow in both conditions. From the 89 isolates, the growth curves under 8 treatments of combined fructose (from 20% to 5%) and ethanol (from 0% to 10%) were obtained, and the kinetic parameters were analyzed with principal component analysis and k‐means clustering. The resulting yeast strain groups corresponded to the fast, medium and slow growers. A second clustering of only the fast growers led to the selection of 3 Saccharomyces strains (199, 230, 231) that were able to grow rapidly in 4 out of the 8 conditions evaluated. This methodology differentiated strains phenotypically and could be further used for strain selection in other processes.Practical ApplicationA method to select yeast strains for fermentation taking into account the natural differences of yeast isolates. This methodology is based on the cell exposition to combinations of sugar and ethanol, which are the most important stress factors in fermentation. This strategy will help to identify the most tolerant strain that could improve ethanol yield and reduce fermentation time.
Stability and Antioxidant Activity of Annatto (Bixa orellana L.) Tocotrienols During Frying and in Fried Tortilla Chips
Authors: Winkler‐Moser Jill K. , Bakota Erica L. , Hwang Hong‐Sik 
Published: January 2018

AbstractAnnatto tocotrienols (AnT3), which contain approximately 90% δ‐tocotrienol (δ‐T3), were added to mid‐oleic sunflower oil used for frying tortilla chips over 3 d. The objectives were to evaluate their stability during frying, absorption by the fried food, and activity as antioxidants in frying oil and in tortilla chips during storage. AnT3 did not significantly affect the stability of the oil during frying or the sensory profiles of freshly fried chips. The naturally present α‐tocopherol (α‐T) in the oil degraded at a lower rate in the presence of AnT3, resulting in significantly higher α‐T by the end of the frying study. Levels of tocopherols and tocotrienols in the chips mirrored oil levels. AnT3 did not affect the sensory profile of the chips after 1 wk of storage at 50 °C, but after 3 wk of storage, the control chips had higher levels of painty and rancid flavors compared to chips with AnT3. Headspace hexanal was also significantly higher in the control chips compared to the chips with AnT3 after 3 wk of storage.Practical ApplicationAnnatto tocotrienols, containing primarily delta‐ and gamma‐tocotrienols, were added to mid‐oleic sunflower oil used for frying tortilla chips. The tocotrienols were absorbed by the chips along with the oil. They slowed the degradation of α tocopherol during frying, and reduced levels of painty and rancid flavor scores as well as headspace hexanal in chips that were stored for 3 wk at elevated temperatures. The results indicated that fried snack foods such as tortilla chips may be a suitable and convenient vehicle for enriching tocotrienols in the diet, and that tocotrienols may also enhance the shelf‐life of fried foods.
Senescence chips for ultrahigh‐throughput isolation and removal of senescent cells
Authors: Chen Yuchao , Mao Pan , Snijders Antoine M. , Wang Daojing 
Published: January 2018

SummaryCellular senescence plays an important role in organismal aging and age‐related diseases. However, it is challenging to isolate low numbers of senescent cells from small volumes of biofluids for downstream analysis. Furthermore, there is no technology that could selectively remove senescent cells in a high‐throughput manner. In this work, we developed a novel microfluidic chip platform, termed senescence chip, for ultrahigh‐throughput isolation and removal of senescent cells. The core component of our senescence chip is a slanted and tunable 3D micropillar array with a variety of shutters in the vertical direction for rapid cell sieving, taking advantage of the characteristic cell size increase during cellular senescence. The 3D configuration achieves high throughput, high recovery rate, and device robustness with minimum clogging. We demonstrated proof‐of‐principle applications in isolation and enumeration of senescent mesenchymal stem cells (MSCs) from undiluted human whole blood, and senescent cells from mouse bone marrow after total body irradiation, with the single‐cell resolution. After scale‐up to a multilayer and multichannel structure, our senescence chip achieved ultrahigh‐throughput removal of senescent cells from human whole blood with an efficiency of over 70% at a flow rate of 300 ml/hr. Sensitivity and specificity of our senescence chips could be augmented with implementation of multiscale size separation, and identification of background white blood cells using their cell surface markers such as CD45. With the advantages of high throughput, robustness, and simplicity, our senescence chips may find wide applications and contribute to diagnosis and therapeutic targeting of cellular senescence.
Neonatal Ethanol Exposure Causes Behavioral Deficits in Young Mice
Authors: Xu Wenhua , Hawkey Andrew B. , Li Hui , Dai Lu , Brim Howard H. , Frank Jacqueline A. , Luo Jia , Barron Susan , Chen Gang 
Published: January 2018

BackgroundFetal ethanol (EtOH) exposure can damage the developing central nervous system and lead to cognitive and behavioral deficits, known as fetal alcohol spectrum disorders (FASD). EtOH exposure to mouse pups during early neonatal development was used as a model of EtOH exposure that overlaps the human third‐trimester “brain growth spurt”—a model that has been widely used to study FASD in rats.MethodsC57BL/6 male and female mice were exposed to EtOH (4 g/kg/d) on postnatal days (PD) 4 to 10 by oral intubation. Intubated and nontreated controls were also included. Behavioral testing of the offspring, including open field, elevated plus maze, and Morris water maze, was performed on PD 20 to 45.ResultsEtOH exposure during PD 4 to 10 resulted in hyperactivity and deficits in learning and memory in young mice with no apparent sex differences.ConclusionsBased on these data, this neonatal intubation mouse model may be useful for future mechanistic and genetic studies of FASD and for screening of novel therapeutic agents.
Donepezil Reverses Dendritic Spine Morphology Adaptations and Fmr1 Epigenetic Modifications in Hippocampus of Adult Rats After Adolescent Alcohol Exposure
Authors: Mulholland Patrick J. , Teppen Tara L. , Miller Kelsey M. , Sexton Hannah G. , Pandey Subhash C. , Swartzwelder H. Scott 
Published: January 2018

BackgroundAdolescent intermittent ethanol (AIE) exposure produces persistent impairments in cholinergic and epigenetic signaling and alters markers of synapses in the hippocampal formation, effects that are thought to drive hippocampal dysfunction in adult rodents. Donepezil (Aricept), a cholinesterase inhibitor, is used clinically to ameliorate memory‐related cognitive deficits. Given that donepezil also prevents morphological impairment in preclinical models of neuropsychiatric disorders, we investigated the ability of donepezil to reverse morphological and epigenetic adaptations in the hippocampus of adult rats exposed to AIE. Because of the known relationship between dendritic spine density and morphology with the fragile X mental retardation 1 (Fmr1) gene, we also assessed Fmr1 expression and its epigenetic regulation in hippocampus after AIE and donepezil pretreatment.MethodsAdolescent rats were administered intermittent ethanol for 16 days starting on postnatal day 30. Rats were treated with donepezil (2.5 mg/kg) once a day for 4 days starting 20 days after the completion of AIE exposure. Brains were dissected out after the fourth donepezil dose, and spine analysis was completed in dentate gyrus granule neurons. A separate cohort of rats, treated identically, was used for molecular studies.ResultsAIE exposure significantly reduced dendritic spine density and altered morphological characteristics of subclasses of dendritic spines. AIE exposure also increased mRNA levels and H3‐K27 acetylation occupancy of the Fmr1 gene in hippocampus. Treatment of AIE‐exposed adult rats with donepezil reversed both the dendritic spine adaptations and epigenetic modifications and expression of Fmr1.ConclusionsThese findings indicate that AIE produces long‐lasting decreases in dendritic spine density and changes in Fmr1 gene expression in the hippocampal formation, suggesting morphological and epigenetic mechanisms underlying previously reported behavioral deficits after AIE. The reversal of these effects by subchronic, post‐AIE donepezil treatment indicates that these AIE effects can be reversed by up‐regulating cholinergic function.
Increasing the knowledge, identification and treatment of osteoporosis through education and shared decision‐making with residents living in a retirement village community
Authors: Anderson‐Wurf Jane , Harding Catherine , Seal Alexa 
Published: January 2018

ObjectiveThis pilot study explored whether individual goal setting in a retirement village setting could improve strategies to strengthen bones in an ageing population and help prevent osteoporosis.MethodsA two‐phased osteoporosis prevention program was developed, piloted and evaluated involving a group education session followed by the development of individualised Bone Plans based upon personal understanding of individual fracture risk and lifestyle factors.ResultsA significant improvement in knowledge and understanding of factors to prevent and manage osteoporosis was achieved, and changes in lifestyle behaviours were sustained at six months.ConclusionSuccess was due to education by specialist medical and health personnel, flexibility of goal setting, use of group sessions and location of the program within the retirement community setting. The ‘Mind Your Bones’ program is a feasible and acceptable way to translate preventative bone health messages to a large number of people via the retirement village network.
The EAACI/GA²LEN/EDF/WAO guideline for the definition, classification, diagnosis and management of urticaria
Authors: Zuberbier T. , Aberer W. , Asero R. , Abdul Latiff A. H. , Baker D. , Ballmer‐Weber B. , Bernstein J. A. , Bindslev‐Jensen C. , Brzoza Z. , Buense Bedrikow R. , Canonica G. W. , Church M. K. , Craig T. , Danilycheva I. V. , Dressler C. , Ensina L. F. , Giménez‐Arnau A. , Godse K. , Gonçalo M. , Grattan C. , Hebert J. , Hide M. , Kaplan A. , Kapp A. , Katelaris C. H. , Kocatürk E. , Kulthanan K. , Larenas‐Linnemann D. , Leslie T. A. , Magerl M. , Mathelier‐Fusade P. , Meshkova R. Y. , Metz M. , Nast A. , Nettis E. , Oude‐Elberink H. , Rosumeck S. , Saini S. S. , Sánchez‐Borges M. , Schmid‐Grendelmeier P. , Staubach P. , Sussman G. , Toubi E. , Vena G. A. , Vestergaard C. , Wedi B. , Werner R. N. , Zhao Z. , Maurer M. ,   Endorsed by the following societies: AAAAI, AAD, AAIITO, ACAAI, AEDV, APAAACI, ASBAI, ASCIA, BAD, BSACI, CDA, CMICA, CSACI, DDG, DDS, DGAKI, DSA, DST, EAACI, EIAS, EDF, EMBRN, ESCD, GA²LEN, IAACI, IADVL, JDA, NVvA, MSAI, ÖGDV, PSA, RAACI, SBD, SFD, SGAI, SGDV, SIAAIC, SIDeMaST, SPDV, TSD, UNBB, UNEV and WAO
Published: January 2018

AbstractThis evidence‐ and consensus‐based guideline was developed following the methods recommended by Cochrane and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) working group. The conference was held on 1 December 2016. It is a joint initiative of the Dermatology Section of the European Academy of Allergology and Clinical Immunology (EAACI), the EU‐founded network of excellence, the Global Allergy and Asthma European Network (GA²LEN), the European Dermatology Forum (EDF) and the World Allergy Organization (WAO) with the participation of 48 delegates of 42 national and international societies. This guideline was acknowledged and accepted by the European Union of Medical Specialists (UEMS). Urticaria is a frequent, mast cell‐driven disease, presenting with wheals, angioedema, or both. The lifetime prevalence for acute urticaria is approximately 20%. Chronic spontaneous urticaria and other chronic forms of urticaria are disabling, impair quality of life and affect performance at work and school. This guideline covers the definition and classification of urticaria, taking into account the recent progress in identifying its causes, eliciting factors and pathomechanisms. In addition, it outlines evidence‐based diagnostic and therapeutic approaches for the different subtypes of urticaria.
Choroidal thickness and myopia in relation to physical activity – theCHAMPSEye Study
Authors: Lundberg Kristian , Vestergaard Anders Højslet , Jacobsen Nina , Suhr Thykjær Anne , Søgaard Hansen Rasmus , Goldschmidt Ernst , Peto Tunde , Halekoh Ulrich , Wedderkopp Niels , Grauslund Jakob 
Published: January 2018

AbstractPurposeTo describe the relationship between choroidal thickness (CT) and myopia in relation to physical activity (PA) in a population‐based child cohort.MethodsIn a prospective study of 307 children from theCHAMPSStudy Denmark, we used objective data from GT3X accelerometer worn at four periods between 2009 and 2015 to determine the amount and intensity ofPA. Intensity was estimated as counts/minutes, and cut‐off points were defined at four intensity levels. Eye examinations were performed in 2015 and included autorefraction in cycloplegia, axial length (AL) by biometric and fovea‐centred enhanced depth imaging optical coherence tomography. By a semi‐automated method, we measured theCTat 17 targets per eye representing anatomically different locations (subfoveal, 1 and 3 millimetre in each direction of fovea).ResultsMean age at the eye examination was 15.4 ± 0.7 years. The meanALwas 23.5 ± 0.9 mm, and the mean subfovealCTwas 369 ± 87 μm. Choroidal thickness (CT) was 331 ± 68 μm for the overall macula, 355 ± 78 μm for the 1‐mm zone and 304 ± 60 μm for the 3‐mm zone. AllCTmeasurements were thinner in myopic eyes (p < 0.0001) and in boys (p < 0.05). We found no association between totalPAand theCTby either mixed model analysis (p = 0.074) or linear regression by any intensity levels (p = 0.22, p = 0.15 and p = 0.43).ConclusionAmong adolescents aged 14–17 years, there was no association between objective PA exposures and the CT, AL or refractive error.
Statin use and vitreoretinal surgery: Findings from a Finnish population‐based cohort study
Authors: Loukovaara Sirpa , Sahanne Sari , Takala Annika , Haukka Jari 
Published: January 2018

AbstractPurposeVitreoretinal (VR) surgery is the third most common intraocular surgery after refractive and cataract surgery. The impact of statin therapy on VR surgery outcomes remains unclear, despite a potentially beneficial effect. We explored the association of preoperative statin therapy and the need for revitrectomy after primary vitrectomy.MethodsOur historical, population‐based, register‐based, VR surgery cohort consisted of 5709 patients operated in a tertiary, academic referral hospital in Finland, during 2008–2014, covering 6.5 years.Subgroup analysis was performed as follows: eyes operated due to (i) rhegmatogenous retinal detachment (RRD), (ii) VR interface diseases (macular pucker/hole), (iii) diabetic maculopathy or proliferative retinopathy, (iv) vitreous haemorrhage, (v) lens subluxation, (vi) vitreous opacities or (vii) other VR indication.The primary end‐point event was revitrectomy during a postoperative follow‐up period of 1 year due to retinal redetachment, vitreous rehaemorrhage, postoperative endophthalmitis, recurrent pucker or unclosed macular hole.ResultsRhegmatogenous retinal detachment (RRD) was the second most frequent indication of VR surgery, including 1916 patients, with 305 re‐operations with rate 0.20 (95% CI 0.18–0.23) per person‐year. Statin treatment in time of operation was associated with lower risk of re‐operation according to relative scale (incidence rate ratio 0.72, 95% CI 0.53–0.97), but not in absolute scale (incidence rate difference −0.58, 95% CI −4.30 to 3.15 for 100 person‐years). No association with statin therapy and vitrectomy outcome was observed in the other VR subgroups.ConclusionUse of statin treatment was associated with a 28% lower risk of revitrectomy in patients operated due to RRD. Further randomized clinical trials are highly warranted.
Physical exercise and glaucoma: a review on the roles of physical exercise on intraocular pressure control, ocular blood flow regulation, neuroprotection and glaucoma‐related mental health
Authors: Zhu Ming Ming , Lai Jimmy Shiu Ming , Choy Bonnie Nga Kwan , Shum Jennifer Wei Huen , Lo Amy Cheuk Yin , Ng Alex Lap Ki , Chan Jonathan Cheuk Hung , So Kwok Fai 
Published: January 2018

AbstractThe benefits of physical exercise on health and well‐being have been studied in a wide range of systemic and ocular diseases, including glaucoma, a progressive optic neuropathy characterized by accelerated apoptosis of retinal ganglion cells (RGCs). Elevated intraocular pressure (IOP) and insufficient ocular perfusion have been postulated to be the two main theories in glaucoma development and progression. The effects of exercise in these two aspects have been demonstrated by numerous researches. A review in 2009 focusing on these two theories concluded that exercise results in transient IOP reduction but an inconsistent elevation in ocular perfusion. However, the majority of the studies had been conducted in healthy subjects. Over the past decade, technological advancement has brought forth new and more detailed evidence regarding the effects of exercise. Moreover, the neuroprotective effect of exercise by upregulation of neurotrophin and enhancement of mitochondrial function has been a focus of interest. Apart from visual impairment, the mental health issues in patients with glaucoma, which include anxiety and depression, should also be addressed. In this review, we mainly focus on publications from the recent years, so as to provide a comprehensive review on the impact of physical exercise on IOP, ocular perfusion, neuroprotection and mental health in patients with glaucoma.