bluuebunny/crossref_metadata_2025_split huggingface dataset:
G-CSF–Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way
Authors: Perobelli Suelen Martins , Mercadante Ana Carolina Terra , Galvani Rômulo Gonçalves , Gonçalves-Silva Triciana , Alves Ana Paula Gregório , Pereira-Neves Antonio , Benchimol Marlene , Nóbrega Alberto , Bonomo Adriana 
Published: October 2016

Abstract
Acute graft-versus-host disease (aGVHD) is the main complication of allogeneic hematopoietic stem cell transplantation, and many efforts have been made to overcome this important limitation. We showed previously that G-CSF treatment generates low-density splenic granulocytes that inhibit experimental aGVHD. In this article, we show that aGVHD protection relies on incoming IL-10+ neutrophils from G-CSF–treated donor spleen (G-Neutrophils). These G-Neutrophils have high phagocytic capacity, high peroxide production, low myeloperoxidase activity, and low cytoplasmic granule content, which accounts for their low density. Furthermore, they have low expression of MHC class II, costimulatory molecules, and low arginase1 expression. Also, they have low IFN-γ, IL-17F, IL-2, and IL-12 levels, with increased IL-10 production and NO synthase 2 expression. These features are in accordance with the modulatory capacity of G-Neutrophils on regulatory T cell (Treg) generation. In vivo, CD25+ Treg depletion shortly after transplantation with splenic cells from G-CSF–treated donors blocks suppression of aGVHD, suggesting Treg involvement in the protection induced by the G-Neutrophils. The immunocompetence and specificity of the semiallogeneic T cells, long-term after the bone marrow transplant using G-Neutrophils, were confirmed by third-party skin graft rejection; importantly, a graft-versus-leukemia assay showed that T cell activity was maintained, and all of the leukemic cells were eliminated. We conclude that G-CSF treatment generates a population of activated and suppressive G-Neutrophils that reduces aGVHD in an IL-10– and Treg-dependent manner, while maintaining immunocompetence and the graft versus leukemia effect.
Mannan-Binding Lectin–Associated Serine Protease 1/3 Cleavage of Pro–Factor D into Factor D In Vivo and Attenuation of Collagen Antibody-Induced Arthritis through Their Targeted Inhibition by RNA Interference–Mediated Gene Silencing
Authors: Banda Nirmal K. , Acharya Sumitra , Scheinman Robert I. , Mehta Gaurav , Coulombe Marilyne , Takahashi Minoru , Sekine Hideharu , Thiel Steffen , Fujita Teizo , Holers V. Michael 
Published: October 2016

Abstract
The complement system is proposed to play an important role in the pathogenesis of rheumatoid arthritis (RA). The complement system mannan-binding lectin–associated serine proteases (MASP)-1/3 cleave pro–factor D (proDf; inactive) into Df (active), but it is unknown where this cleavage occurs and whether inhibition of MASP-1/3 is a relevant therapeutic strategy for RA. In the present study, we show that the cleavage of proDf into Df by MASP-1/3 can occur in the circulation and that inhibition of MASP-1/3 by gene silencing is sufficient to ameliorate collagen Ab–induced arthritis in mice. Specifically, to examine the cleavage of proDf into Df, MASP-1/3–producing Df−/− liver tissue (donor) was transplanted under the kidney capsule of MASP-1/3−/− (recipient) mice. Five weeks after the liver transplantation, cleaved Df was present in the circulation of MASP-1/3−/− mice. To determine the individual effects of MASP-1/3 and Df gene silencing on collagen Ab–induced arthritis, mice were injected with scrambled, MASP-1/3–targeted, or Df-targeted small interfering RNAs (siRNAs). The mRNA levels for MASP-1 and -3 decreased in the liver to 62 and 58%, respectively, in mice injected with MASP-1/3 siRNAs, and Df mRNA decreased to 53% in the adipose tissue of mice injected with Df siRNAs; additionally, circulating MASP-1/3 and Df protein levels were decreased. In mice injected with both siRNAs the clinical disease activity, histopathologic injury scores, C3 deposition, and synovial macrophage/neutrophil infiltration were significantly decreased. Thus, MASP-1/3 represent a new therapeutic target for the treatment of RA, likely through both direct effects on the lectin pathway and indirectly through the alternative pathway.
Evaluation of 2 Heat-Mitigation Methods in Army Trainees
Authors: Sefton JoEllen M. , McAdam J. S. , Pascoe David D. , Lohse K. R. , Banda Robert L. , Henault Corbin B. , Cherrington Andrew R. , Adams N. E. 
Published: October 2016

Context: Heat injury is a significant threat to military trainees. Different methods of heat mitigation are in use across military units. Mist fans are 1 of several methods used in the hot and humid climate of Fort Benning, Georgia.
Objectives: To determine if (1) the mist fan or the cooling towel effectively lowered participant core temperature in the humid environment found at Fort Benning and (2) the mist fan or the cooling towel presented additional physiologic or safety benefits or detriments when used in this environment.
Design: Randomized controlled clinical trial.
Setting: Laboratory environmental chamber.
Patients or Other Participants: Thirty-five physically active men aged 19 to 35 years.
Intervention(s): (1) Mist fan, (2) commercial cooling towel, (3) passive-cooling (no intervention) control. All treatments lasted 20 minutes. Participants ran on a treadmill at 60% V̇o2max.
Main Outcome Measure(s): Rectal core temperature, heart rate, thermal comfort, perceived temperature, perceived wetness, and blood pressure.
Results: Average core temperature increased during 20 minutes of cooling (F1,28 = 64.76, P &lt; .001, ηp2 = 0.70), regardless of group (F1,28 = 3.41, P = .08, ηp2 = 0.11) or condition (F1,28 &lt; 1.0). Core temperature, heart rate, and blood pressure did not differ among the 3 conditions. Perceived temperature during 20 minutes of cooling decreased (F1,30 = 141.19, P &lt; .001, ηp2 = 0.83) regardless of group or condition. Perceived temperature was lower with the mist-fan treatment than with the control treatment (F1,15 = 7.38, P = .02, ηp2 = 0.32). The mist-fan group perceived themselves to be cooler even at elevated core temperatures.
Conclusions: The mist fan and cooling towel were both ineffective at lowering core temperature. Core temperature continued to increase after exercise in all groups. The mist fan produced feelings of coolness while the core temperature remained elevated, possibly increasing the risk of heat illness.
A enfermagem no manejo da dor em unidades de atendimento de urgência e emergência
Authors: Oliveira Paulo Eduardo Pires de , Pereira Lilian Varanda , Santos Nilde Resplandes , Souza Layz Alves Ferreira 
Published: October 2016

A enfermagem deve manejar a dor no atendimento em urgência e emergência, baseando-se em evidências científicas, mas isso parece apresentar obstáculos. Objetivou-se identificar e analisar as estratégias de manejo da dor e as barreiras para sua utilização entre as categorias profissionais da enfermagem nos serviços de urgência e emergência. Revisão integrativa, com buscas no LILACS, SciVerse Scopus, PUBMED e na literatura cinzenta do Google acadêmico. Foram selecionados 15 artigos publicados entre 2012 e 2015. A enfermagem avaliou as características da dor por meio de anamnese e uso de instrumentos. As intervenções restringiram-se prioritariamente às farmacológicas, apontando a necessidade de associá-las às não farmacológicas. A monitorização da dor ainda é incipiente. Os profissionais necessitam avançar no uso de instrumentos válidos para avaliação, intervenção e monitoramento da dor, com o propósito de vencer as barreiras que profissionais, clientes e gerentes das unidades de urgência e emergência encontram para sistematizar o seu manejo.
PROMOÇÃO DO CRESCIMENTO E ENRAIZAMENTO DE MINIESTACAS DE Eucalyptus dunnii POR RIZOBACTÉRIAS E BACTÉRIAS DO FILOPLANO
Authors: Spassin Ana Claudia , Vandresen Paula Burigo , Neves Dyanndra , Garcia Flávio Augusto de Oliveira , Ukan Daniele , Peres Fabiana Schmidt Bandeira 
Published: October 2016

O uso de microrganismos com efeito de promotores de crescimento e enraizamento de plantas tem apresentando resultados promissores no desenvolvimento de mudas clonais. Assim, o objetivo do estudo foi avaliar o potencial de isolados de rizobactérias e residentes do filoplano na promoção de crescimento e enraizamento de miniestacas de Eucalyptus dunnii. O experimento foi conduzido no delineamento de blocos casualizados, em arranjo fatorial 4 x 4 x 3, sendo os fatores constituídos por quatro coletas, quatro isolados bacterianos e três métodos de aplicação. Foram avaliados o percentual de sobrevivência das miniestacas na saída da casa de vegetação, casa de sombra e área de pleno sol e, o enraizamento das miniestacas aos 90 dias de idade. A resposta na sobrevivência de mudas na casa de vegetação, sombra e área de pleno sol variaram de acordo com o isolado, forma de aplicação e período de realização da coleta. Não foi observado efeito significativo dos isolados bacterianos sobre o enraizamento das miniestacas. Os isolados bacterianos apresentaram ora efeito benéfico, ora ausência de efeito para a sobrevivência e enraizamento das miniestacas. Por ser o primeiro relato do uso de rizobactérias e residentes de filoplano na promoção de crescimento de E. dunnii há demanda de novos estudos para a confirmação da efetividade destes microrganismos.
Surgical Excision of Heterotopic Ossification Leads to Re-Emergence of Mesenchymal Stem Cell Populations Responsible for Recurrence
Authors: Agarwal Shailesh , Loder Shawn , Cholok David , Li John , Breuler Chris , Drake James , Brownley Cameron , Peterson Joshua , Li Shuli , Levi Benjamin 
Published: October 2016

Abstract
Trauma-induced heterotopic ossification (HO) occurs after severe musculoskeletal injuries and burns, and presents a significant barrier to patient rehabilitation. Interestingly, the incidence of HO significantly increases with repeated operations and after resection of previous HO. Treatment of established heterotopic ossification is challenging because surgical excision is often incomplete, with evidence of persistent heterotopic bone. As a result, patients may continue to report the signs or symptoms of HO, including chronic pain, nonhealing wounds, and joint restriction. In this study, we designed a model of recurrent HO that occurs after surgical excision of mature HO in a mouse model of hind-limb Achilles’ tendon transection with dorsal burn injury. We first demonstrated that key signaling mediators of HO, including bone morphogenetic protein signaling, are diminished in mature bone. However, upon surgical excision, we have noted upregulation of downstream mediators of osteogenic differentiation, including pSMAD 1/5. Additionally, surgical excision resulted in re-emergence of a mesenchymal cell population marked by expression of platelet-derived growth factor receptor-α (PDGFRα) and present in the initial developing HO lesion but absent in mature HO. In the recurrent lesion, these PDGFRα+ mesenchymal cells are also highly proliferative, similar to the initial developing HO lesion. These findings indicate that surgical excision of HO results in recurrence through similar mesenchymal cell populations and signaling mechanisms that are present in the initial developing HO lesion. These results are consistent with findings in patients that new foci of ectopic bone can develop in excision sites and are likely related to de novo formation rather than extension of unresected bone.
Cooperación universitaria para el desarrollo en Makeni (Sierra Leona)
Authors: Perea Moreno Luis , García Fernández Natalia , Salas Ruiz Adela 
Published: October 2016

El crecimiento acelerado de las ciudades medias africanas de menos de 500.000 habitantes, representa el principal desafío urbano del momento a nivel mundial. Su desarrollo implicará, en las próximas décadas, un notable incremento de suelo urbano en los países en vías de desarrollo. La falta de recursos, de un marco normativo adecuado, de profesionales en planificación urbana y los déficits de habitabilidad ya existentes en estos entornos, son realidades que evidencian, entre otras, la urgencia de articular un importante esfuerzo colectivo. El planeamiento urbano y territorial convencional no es capaz de responder a estos retos en tiempo y escala. Por una parte, por la velocidad vertiginosa de la progresiva toma de tierra y el incesante crecimiento de la población y, por otra, por la dificultad que supone para la población asumir propuestas de planificación con visos de futuro en contextos en los que no existe cultura en planeamiento. Así entre Madrid (España) y Makeni (Sierra Leona) surge una experiencia en la que la Universidad y la Cooperación al Desarrollo emergen como elementos críticos de fortalecimiento institucional y formativo. La colaboración entre Universidades (USPCEU-UNIMAK), es el germen de un proceso de reflexión urbana que implica decididamente al Ayuntamiento y a los principales actores locales de la ciudad.
Disidencias artísticas en Ciudad Juárez. Prácticas colaborativas en contextos de violencia.
Authors: Méndez Llopis Carles 
Published: October 2016

Ciudad Juárez, como tantas otras regiones mexicanas, ha sido violentada por múltiples y heterogéneas acciones delictivas que se han sumado al desconcierto y hartazgo de una sociedad con autoridades sin dimensión pública, que siguen ahogadas en su incapacidad e insuficiencia surrealista. Una urdimbre complejizada por su estado fronterizo con Estados Unidos, que la hace, a su vez, ser ciudad-puente, un espacio para el nomadismo y el tránsito de residentes, de ideas, de contactos, etc. Un contexto en el que, si bien las élites políticas y económicas son ofensivas al bien común, los ciudadanos concentran sus esfuerzos en cultivar la habitabilidad y la cohesión urbana, en dar sentido a la vivencia y memoria colectiva, y en ocupar sus centros para hacer de Ciudad Juárez algo más que una simple escenografía vacía. Insertos en estos movimientos de reconstrucción se posicionan diversos colectivos y agentes artísticos que tomando la metrópoli como proyecto y soporte de sus estrategias van integrando nuevas dimensiones humanas y ciudadanas a través de la sensibilidad. La presentación y el texto abordarán las diferentes tácticas de estos dispositivos de activación urbana.
A systematic review of measures of HIV/AIDS stigma in paediatric HIV‐infected and HIV‐affected populations
Authors: McAteer Carole Ian , Truong Nhan‐Ai Thi , Aluoch Josephine , Deathe Andrew Roland , Nyandiko Winstone M , Marete Irene , Vreeman Rachel Christine 
Published: October 2016

IntroductionHIV‐related stigma impacts the quality of life and care management of HIV‐infected and HIV‐affected individuals, but how we measure stigma and its impact on children and adolescents has less often been described.MethodsWe conducted a systematic review of studies that measured HIV‐related stigma with a quantitative tool in paediatric HIV‐infected and HIV‐affected populations.Results and discussionVarying measures have been used to assess stigma in paediatric populations, with most studies utilizing the full or variant form of the HIV Stigma Scale that has been validated in adult populations and utilized with paediatric populations in Africa, Asia and the United States. Other common measures included the Perceived Public Stigma Against Children Affected by HIV, primarily utilized and validated in China. Few studies implored item validation techniques with the population of interest, although scales were used in a different cultural context from the origin of the scale.ConclusionsMany stigma measures have been used to assess HIV stigma in paediatric populations, globally, but few have implored methods for cultural adaptation and content validity.
Effects of chirp of pump pulses on broadband terahertz pulse spectra generated by optical rectification
Authors: Hamazaki Junichi , Furusawa Kentaro , Sekine Norihiko , Kasamatsu Akifumi , Hosako Iwao 
Published: October 2016

Abstract
The effects of the chirp of the pump pulse in broadband terahertz (THz) pulse generation by optical rectification (OR) in GaP were systematically investigated. It was found that the pre-compensation for the dispersion of GaP is important for obtaining smooth and single-peaked THz spectra as well as high power-conversion efficiency. It was also found that an excessive amount of chirp leads to distortions in THz spectra, which can be quantitatively analyzed by using a simple model. Our results highlight the importance of accurate control over the chirp of the pump pulse for generating broadband THz pulses by OR.
Effects of drain doping concentration on switching characteristics of tunnel field-effect transistor inverters
Authors: Kwon Dae Woong , Kim Jang Hyun , Park Byung-Gook 
Published: October 2016

Abstract
In order to investigate the effects of the modulation of drain doping concentration (N
drain) on alternating current (AC) switching characteristics of a tunnel filed-effect transistor (TFET) inverter, the characteristics of TFETs with various N
drains are analyzed rigorously through mixed-mode device and circuit TCAD simulations. As the N
drain gets decreased, the drain current (I
D) becomes reduced and the gate-to-drain capacitance (C
GD) reflects the entire gate capacitance (C
GG) at a lower gate voltage (V
G), which leads to the degradation of falling/rising delay in TFET inverters. These phenomena are explained successfully by the change of quasi-Fermi energy in the drain (E
F_drain) as a function of V
G. The E
F_drain rises dramatically from when tunneling current starts to flow from the source in the n-type TFET with low N
drain. As a result, drain-side channel inversion occurs at a lower V
G due to the reduction of the energy barrier between the E
F_drain and the conduction band edge of the channel.
Associations between different components of fitness and fatness with academic performance in Chilean youths
Authors: Olivares Pedro R. , García-Rubio Javier 
Published: October 2016

ObjectivesTo analyze the associations between different components of fitness and fatness with academic performance, adjusting the analysis by sex, age, socio-economic status, region and school type in a Chilean sample.MethodsData of fitness, fatness and academic performance was obtained from the Chilean System for the Assessment of Educational Quality test for eighth grade in 2011 and includes a sample of 18,746 subjects (49% females). Partial correlations adjusted by confounders were done to explore association between fitness and fatness components, and between the academic scores. Three unadjusted and adjusted linear regression models were done in order to analyze the associations of variables.ResultsFatness has a negative association with academic performance when Body Mass Index (BMI) and Waist to Height Ratio (WHR) are assessed independently. When BMI and WHR are assessed jointly and adjusted by cofounders, WHR is more associated with academic performance than BMI, and only the association of WHR is positive. For fitness components, strength was the variable most associated with the academic performance. Cardiorespiratory capacity was not associated with academic performance if fatness and other fitness components are included in the model.ConclusionsFitness and fatness are associated with academic performance. WHR and strength are more related with academic performance than BMI and cardiorespiratory capacity.
Improving education and coping of scoliosis patients undergoing surgery, and their families, using e-health
Authors: Lysenko Magdalena , Law Peggy , Jarvis James , Wright James G. 
Published: October 2016

Purpose Healthcare providers have limited time to spend with scoliosis patients who are considering surgery and their families. The purpose of this study was to evaluate an e-health strategy to increase knowledge and coping in patients with scoliosis who are surgical candidates and their families. Methods We enrolled patients with scoliosis who were candidates for surgery and their families. Patients and their families completed the scoliosis knowledge questionnaire, meaning of illness questionnaire, social support and coping questionnaires before and after access to a comprehensive evidence-based scoliosis website ( http://www.aboutkidshealth.ca/scoliosis ). Results Seventy-four patients and 71 parents completed the evaluation. While both patients and parents improved their knowledge of scoliosis ( p = 0.001 and p = 0.003, respectively), the scores of patients were consistently lower than those of the parents both before and after website use ( p = 0.0001). Only parents demonstrated a change in the meaning of illness questionnaire, with a small increase in the negative attitude towards illness and a small decrease in the positive attitude towards illness ( p = 0002 and p = 0.01, respectively). Of the 12 coping methods examined on the Adolescent Coping Orientation for Problem Experiences (A-COPE) instrument, patients were slightly more likely than parents to use relaxing and solving family problems as tools to cope following website access ( p = 0.02 and p = 0.09, respectively). Parents demonstrated no significant changes in the four methods of coping on the Coping Health Inventory for Parents (CHIP) after website exposure. While the majority of patients and parents reported receiving sufficient support, over half of the patients indicated a need for more support in social participation. Conclusion An evidence-based website increased the knowledge of patients and parents but simply providing access to the website had minimal impact on their coping and perceptions of social support. The website, however, provides users with the opportunity to absorb vital information about scoliosis across several media.
The sense of relational entitlement among adolescents toward their parents (SREap) – Testing an adaptation of the SRE
Authors: Tolmacz Rami , Efrati Yaniv , Ben‐David Boaz M. 
Published: October 2016

ABSTRACTThe quality of the adolescent–parent relationship is closely related to the adolescent's sense of entitlement. Study 1 (458 central‐Israel adolescents, 69% girls, ages: 11–16) developed the sense of relational entitlement among adolescents toward their parents (SREap, adapted from the original SRE on adults' romantic relationships) and provided initial validity evidence of its three‐factor structure: exaggerated, restricted and assertive – replicating the SRE's factor structure. Studies 2–5 (1237 adolescents, 56% girls) examined the link between the SREap factors and relevant psychological measures. Exaggerated and restricted SREap factors were associated with attachment insecurities. Restricted and exaggerated entitlement factors were related to higher levels of emotional problems, and lower levels of: wellbeing, positive mood and life satisfaction. Conversely, assertive entitlement was related to higher life satisfaction and self‐efficacy and lower levels of emotional problems. The findings also indicate that SREap is not merely a form of narcissism. The implications of SREap are discussed.