bluuebunny/crossref_metadata_2025_split huggingface dataset:
From the analysis of work-processes to designing competence-based occupational standards and vocational curricula
Authors: Tūtlys Vidmantas , Spöttl Georg 
Published: December 2016

Purpose
This paper aims to explore methodological and institutional challenges on application of the work-process analysis approach in the design and development of competence-based occupational standards for Lithuania.

Design/methodology/approach
The theoretical analysis is based on the review of scientific literature and the analysis of documents and methodical instruments (curricula and occupational standards). Empirical research is based on the observation and analysis of the processes of designing work-process-based occupational standards for Lithuania, including the face-to-face interviews with involved work-process experts on the shop-floor and stakeholders.

Findings
The application of a work-process-based approach in designing sectoral occupational standards enhances comprehensive and systemic design of qualifications. Work-process analysis approach helps to focus on the holistic concept of competence by considering different dimensions of work-processes. However, design and implementation of work-process-based occupational standards for the transitional and predominantly school-based vocational education and training (VET) systems encounter multiple methodological and institutional challenges.

Research limitations/implications
The findings of research are based on the analysis and evaluation of the design of sectoral-occupational standards in the beginning and middle stages of this process. These findings can help to draw the assumptions about potential implications of implementation of these standards to the development of competence-based VET but are not sufficient to provide comprehensive and detailed forecasts.

Originality/value
The paper explores and evaluates an application of the innovative work process approach in the design and development of qualifications for the concrete country.
Impulse control disorder, lysosomal malfunction andATP13A2 insufficiency in Parkinsonism
Authors: Liu Jun‐Ping , Li Jianfeng , Lu Yanhua , Wang Lihui , Chen Gang 
Published: December 2016

AbstractLysosomal transport of cargos in neurons is essential for neuronal proteostasis, transmission and functional motors and behaviours. Lysosomal malfunction including storage disorders is involved in the pathogenesis of Parkinson's disease (PD). Given the unclear molecular mechanisms of diverse defects inPDphenotypes, especially behavioural deficits, this mini review explores the cellular contexts ofPDimpulse control disorders and the molecular aspects of lysosomal cross‐membrane transports. Focuses are paid to trace metal involvements in α‐synuclein assembly in Lewy bodies, the functions and molecular interactions ofATP13A2 asATPase transporters in lysosomal membranes for cross‐membrane trafficking and lysosomal homeostasis, and our current understandings of the neural circuits inICD. Erroneously polarized distributions of cargos such as metals and lipids on each side of lysosomal membranes triggered by gene mutations and deregulated expression ofATP13A2 may thus instigate sensing protein structural changes such as aggregations, organelle degeneration, and specific neuronal ageing and death in Parkinsonism.
Sialotranscriptome sequencing and analysis of Anopheles sinensis and comparison with Psorophora albipes sialotranscriptome (Diptera: Culicidae)
Authors: Fang Ya‐Jie , Yan Zhen‐Tian , Chen Bin 
Published: December 2016

AbstractMost of adult female mosquitoes secrete saliva to facilitate blood sucking, digestion and nutrition, and mosquito‐borne disease prevention. The knowledge of classification and characteristics of sialotranscriptome genes are still quite limited. Anopheles sinensis is a major malaria vector in China and southeast Asian countries. In this study, the An. sinensis sialotranscriptome was sequenced using Illumina sequencing technique with a total of 10 907 unigenes to be obtained and annotated in biological functions and pathways, and 10 470 unigenes were mapped to An. sinensis reference genome with 70.46% of genes having 90%–100% genome mapping through bioinformatics analysis. These mapped genes were classified into four categories: housekeeping (6632 genes), secreted (1177), protein‐coding genes with function‐unknown (2646) and transposable element (15). The housekeeping genes were divided into 27 classes, and the secreted genes were divided into 11 classes and 96 families. The classification, characteristics and evolution of these classes/families of secreted genes are further described and discussed. The comparison of the 1177 secreted genes in An. sinensis in the Anophelinae subfamily with 811 in Psorophora albipes in the Culicinae subfamily show that six classes/subclasses have the gene number more than twice and two classes (uniquely found in anophelines, and Orphan proteins of unique standing) are unique in the former compared with the latter, whereas four classes/subclasses are much expanded and uniquely found in the Aedes class and is unique in the later. The An. sinensis sialotranscriptome sequence data is the most complete in mosquitoes to date, and the analyses provide a comprehensive information frame for further research of mosquito sialotranscriptome.
Reconstructing the plant mitochondrial genome for marker discovery: a case study using Pinus
Authors: Donnelly Kevin , Cottrell Joan , Ennos Richard A. , Vendramin Giovanni Giuseppe , A'Hara Stuart , King Sarah , Perry Annika , Wachowiak Witold , Cavers Stephen 
Published: December 2016

AbstractWhole‐genome‐shotgun (WGS) sequencing of total genomic DNA was used to recover ~1 Mbp of novel mitochondrial (mtDNA) sequence from Pinus sylvestris (L.) and three members of the closely related Pinus mugo species complex. DNA was extracted from megagametophyte tissue from six mother trees from locations across Europe, and 100‐bp paired‐end sequencing was performed on the Illumina HiSeq platform. Candidate mtDNA sequences were identified by their size and coverage characteristics, and by comparison with published plant mitochondrial genomes. Novel variants were identified, and primers targeting these loci were trialled on a set of 28 individuals from across Europe. In total, 31 SNP loci were successfully resequenced, characterizing 15 unique haplotypes. This approach offers a cost‐effective means of developing marker resources for mitochondrial genomes in other plant species where reference sequences are unavailable.
Dentate gyrus−cornu ammonis (CA) 4 volume is decreased and associated with depressive episodes and lipid peroxidation in bipolar II disorder: Longitudinal and cross‐sectional analyses
Authors: Elvsåshagen Torbjørn , Zuzarte Pedro , Westlye Lars T , Bøen Erlend , Josefsen Dag , Boye Birgitte , Hol Per K , Malt Ulrik F , Young L Trevor , Andreazza Ana C 
Published: December 2016

ObjectivesReduced dentate gyrus volume and increased oxidative stress have emerged as potential pathophysiological mechanisms in bipolar disorder. However, the relationship between dentate gyrus volume and peripheral oxidative stress markers remains unknown. Here, we examined dentate gyrus–cornu ammonis (CA) 4 volume longitudinally in patients with bipolar II disorder (BD‐II) and healthy controls and investigated whether BD‐II is associated with elevated peripheral levels of oxidative stress.MethodsWe acquired high‐resolution structural 3T‐magnetic resonance imaging (MRI) images and quantified hippocampal subfield volumes using an automated segmentation algorithm in individuals with BD‐II (n=29) and controls (n=33). The participants were scanned twice, at study inclusion and on average 2.4 years later. In addition, we measured peripheral levels of two lipid peroxidation markers (4‐hydroxy‐2‐nonenal [4‐HNE] and lipid hydroperoxides [LPH]).ResultsFirst, we demonstrated that the automated hippocampal subfield segmentation technique employed in this work reliably measured dentate gyrus–CA4 volume. Second, we found a decreased left dentate gyrus–CA4 volume in patients and that a larger number of depressive episodes between T1 and T2 predicted greater volume decline. Finally, we showed that 4‐HNE was elevated in BD‐II and that 4‐HNE was negatively associated with left and right dentate gyrus–CA4 volumes in patients.ConclusionsThese results are consistent with a role for the dentate gyrus in the pathophysiology of bipolar disorder and suggest that depressive episodes and elevated oxidative stress might contribute to hippocampal volume decreases. In addition, these findings provide further support for the hypothesis that peripheral lipid peroxidation markers may reflect brain alterations in bipolar disorders.
Alzheimer neuropathology without frontotemporal lobar degeneration hallmarks (TAR DNA‐binding protein 43 inclusions) in missense progranulin mutation Cys139Arg
Authors: Redaelli Veronica , Rossi Giacomina , Maderna Emanuela , Kovacs Gabor G. , Piccoli Elena , Caroppo Paola , Cacciatore Francesca , Spinello Sonia , Grisoli Marina , Sozzi Giuliano , Salmaggi Andrea , Tagliavini Fabrizio , Giaccone Giorgio 
Published: December 2016

AbstractNull mutations in progranulin gene (GRN) reduce the progranulin production resulting in haploinsufficiency and are tightly associated with tau‐negative frontotemporal lobar degeneration with TAR DNA‐binding protein 43‐positive inclusions (FTLD‐TDP). Missense mutations of GRN were also identified, but their effects are not completely clear, in particular unanswered is the question of what neuropathology they elicit, also considering that their occurrence has been reported in patients with typical clinical features of Alzheimer disease. They describe two fraternal twins carrying the missense GRN Cys139Arg mutation affected by late‐onset dementia and we report the neuropathological study of one of them. Both patients were examined by neuroimaging, neuropsychological assessment and genetic analysis of GRN and other genes associated with dementia. The brain of one was obtained at autopsy and examined neuropathologically. One sister presented clinical and MRI features leading to the diagnosis of Alzheimer disease. The other underwent autopsy and the brain showed neuropathological hallmarks of Alzheimer disease with abundant Aβ‐amyloid deposition and Braak stage V of neurofibrillary pathology, in the absence of the hallmark lesions of FTLD‐TDP. Their findings may contribute to better clarify the role of progranulin in neurodegenerative diseases indicating that some GRN mutations, in particular missense ones, may act as strong risk factor for Alzheimer disease rather than induce FTLD‐TDP.
Degree of methylation burden is determined by the exposure period to carcinogenic factors
Authors: Takeshima Hideyuki , Niwa Tohru , Toyoda Takeshi , Wakabayashi Mika , Yamashita Satoshi , Ushijima Toshikazu 
Published: December 2016

Aberrant DNA methylation accumulated in normal tissues, namely methylation burden, is associated with risk of carcinogenesis. The levels of methylation burden are known to be influenced by multiple factors, such as genetic factors and strengths of carcinogenic factors. However, the impact of the degree of exposure to a carcinogenic factor is still unclear. Here, using a Mongolian gerbil model of Helicobacter pylori (H. pylori)‐induced gastritis, we aimed to clarify the impact of the degree of exposure on methylation burden in normal gastric tissues. DNA methylation levels of four CpG islands, HE6, SA9, SB5, and SD2, increased by H. pylori infection, depending upon the infection period. After eradication of H. pylori, DNA methylation levels decreased, but tended to be higher in gastric mucosae with a longer infection period. DNA molecules with dense methylation, but not those with sparse methylation, increased depending upon the infection period. DNA methylation levels of one of the four CpG islands, SA9, tended to be higher in gastric mucosae of gerbils infected with H. pylori, even 50 weeks after eradication than in those of non‐infected gerbils. These results showed for the first time that the levels of methylation burden in normal tissues are influenced by the degree of exposure to a carcinogenic factor.
‘It's complicated.’ Exploring the meaning of sibling relationships of youth in foster care
Authors: Wojciak Armeda Stevenson 
Published: December 2016

AbstractYouth in foster care may be separated from their sibling(s) for a myriad of reasons. Although empirical attention to sibling relationships has grown, an examination of what sibling(s) mean to children currently in foster care has not occurred. This study used open‐ended survey responses of campers who attended Camp To Belong, a summer camp that reunites children who are separated from their sibling because of their placement in foster care. Six different member camps across the United States of America administered camper surveys. Thematic analysis was used to examine 178 responses from campers. Five major themes were identified of what siblings mean to youth in foster care: bond, dependable, fulfillment, despair and impact of separation. Youth shared the positive and protective influence their sibling(s) have in their life as well as the challenges that are associated with being separated from their sibling while in foster care. Implications of the results of the study are provided for clinicians, foster parents, researchers and sibling policy.
Health‐related quality of life in adult dermatitis patients stratified by filaggrin genotype
Authors: Heede Nina G. , Thyssen Jacob P. , Thuesen Betina H. , Linneberg Allan , Szecsi Pal B. , Stender Steen , Johansen Jeanne D. 
Published: December 2016

SummaryBackgroundInformation concerning health‐related quality of life (HRQoL) and comorbidities of adult dermatitis patients stratified by loss‐of‐function mutations in the filaggrin gene (FLG) is limited.ObjectiveTo investigate HRQoL, skin symptoms and comorbidities in adult FLG mutation carriers.MethodsThis cross‐sectional study included patients diagnosed with atopic dermatitis and/or hand eczema (n = 520). Patients completed questionnaires about dermatitis, skin symptoms, HRQoL, and comorbidities, including actinic keratosis, and atopic and mental disorders.ResultsFLG mutations (R501X, 2282del4, and R2447X) were identified in 16.9% of patients, and were significantly associated not only with atopic dermatitis, but also independently with skin fissures on the fingers and heels, and self‐reported actinic keratosis. Although FLG mutations were significantly associated with reduced HRQoL, as measured by use of the Dermatology Life Quality Index (DLQI), no association with self‐reported anxiety or depression was identified. Notably, the highest median DLQI score, reflecting greater impairment, was reported by patients with both FLG mutations and atopic dermatitis. Overall, 19.7% of patients with both atopic dermatitis and FLG mutations reported a ‘large or extremely large’ impact on their lives; this represents twice the prevalence seen in patients with atopic dermatitis and wild‐type FLG (9.6%).ConclusionPatients with both atopic dermatitis and common FLG mutations are more frequently affected by reduced HRQoL.
Medium‐term assessment of cardiac function in pediatric cancer survivors. Comparison of different echocardiographic methods, cardiac MRI and cardiac biomarker testing in adolescent cancer survivors
Authors: Shah Sanket S. , McClellan Wendy , Knowlton Joshua Q. , Mehta Jeet , Goudar Suma , Ferguson Angela , Sherman Ashley , Shirali Girish , Fulbright Joy 
Published: December 2016

PurposeTo evaluate the feasibility and correlation of 3D echocardiography (echo) and cardiac biomarkers with cardiac MRI, in surveillance of cardiac function for cancer survivors.MethodsSubjects ≥10 years of age who have survived >2 years after completion of cancer treatment from a single center were enrolled. Cardiac MRI and 3D echo images were obtained on the same day when routine echo was obtained. On the same day, along with annual routine blood test, cardiac biomarkers N‐terminal pro‐B‐type natriuretic peptide levels (NT‐proBNP) and troponin‐I levels were also measured.ResultsCardiac MRI was feasible in all 50 subjects. Three‐dimensional echo and 2D echo images were of poor quality in four subjects. With a median duration of remission of 10 years, there were four subjects with mild LV dysfunction (cardiac MRI LV EF of<53%). None had MRI EF <50%, and nine subjects had LVEF <55%. M‐mode echo overestimated EF more than 2D and 3D echo. Two‐dimensional and 3D echo methods had much tighter limits of agreement for LV EF. For measurement of LVEF, 3D echo had a lower % error than 2D echo or M‐mode echo. One subject had an abnormal troponin‐I level and another one had an elevated NT‐proBNP.ConclusionsThree‐dimensional echo can be performed in most adolescent cancer survivors, and it correlates well with MRI. Further large‐scale research is required in assessing utility of cardiac biomarkers in pediatric cancer survivors.
How To Manage Long‐term Financial Self‐sufficiency of a National Catastrophe Insurance Fund? The Feasibility of Three Bailout Programmes
Authors: Wang Jo‐Yu , Wu Wen‐Lin , Wu Yang‐Che , Yang Ming Jing 
Published: December 2016

AbstractThis paper shows the feasibility that a natural catastrophe insurance fund (NCIF) may achieve financial self‐sufficiency via three bailout programmes, including pre‐funding, loan‐financing and equity‐financing, to support the insurers during the bad years. Under such programmes, different accounting procedures for the insurers and NCIF are developed to simulate their 30‐year cash flows based on the best‐fitting loss model calibrated by the global insured loss data. The numerical analysis results indicate that the proposed programmes can balance the financial revenue and expenditure of NCIF in the long term, and this conclusion implies the authority can develop similar schemes as NCIF to smooth the peak risk of natural catastrophes.
Evidence of molybdenum association with particulate organic matter under sulfidic conditions
Authors: Dahl T. W. , Chappaz A. , Hoek J. , McKenzie C. J. , Svane S. , Canfield D. E. 
Published: December 2016

AbstractThe geochemical behavior of molybdenum (Mo) in the oceans is closely linked to the presence of sulfide species in anoxic environments, where Fe availability may play a key role in the Mo scavenging. Here, we show that Mo(VI) is reduced in the presence of particulate organic matter (represented by sulfate‐reducing bacteria). Molybdenum was immobilized at the surface of both living cells and dead/lysed cells, but not in cell‐free control experiments. Experiments were carried out at four different Mo concentrations (0.1 to 2 mm) to yield cell‐associated Mo precipitates with little or no Fe, consisting of mainly Mo(IV)‐sulfide compounds with molecular structures similar to Mo enzymes and to those found in natural euxinic sediments. Therefore, we propose that Mo removal in natural sulfidic waters can proceed via a non‐Fe‐assisted pathway that requires particulate organic matter (dead or living sulfate‐reducing bacteria). This pathway has implications for global marine Mo cycling and the current use of Mo‐based proxies for paleo‐environmental investigations.
‘Canaries in the mine’. Parents of adult children with schizophrenia: An integrative review of the literature
Authors: Klages Debra , Usher Kim , Jackson Debra 
Published: December 2016

AbstractThe purpose of this integrative review was to evaluate the current state of knowledge of parents who have adult children diagnosed with schizophrenia and their relationship with mental health professionals. Findings indicated that parents (primarily mothers) believed they intuitively knew when their adult children were becoming unwell and that they doggedly pursued connections with mental health care providers. Five themes were evident in the literature: trusting your instincts, feeling dismissed and devalued, making connections and making concessions, living with distress and sorrow, and becoming your own health‐care provider. The implications of the findings on mental health nursing practice indicate that professional family relationships were not ideal, and that parents wanted to improve these relationships. Parents wanted health‐care professionals to respond to their requests for help for both their children and for themselves, and wanted to be able to help the mental health team to help their adult children.
The concept of integrated services in different welfare states from a life course perspective
Authors: Minas Renate 
Published: December 2016

AbstractThe concept of integrated services is a common feature of current social policy discussions. It is often argued that social support systems have not evolved to cope with the complexity of individuals’ needs. This is deemed true for a variety of interrelated difficulties that cut across traditional welfare programmes and life course lines. This article examines the efforts of integrated services to bridge policy areas such as social policy, labour market policy and health care services for four different vulnerable groups at major stages of the life course: childhood, youth, adulthood and old age. Analytically, the article adopts a framework developed by Valentijn et al. () that allows systematic comparisons. Using mainly high‐income economy examples, the article connects key features of a certain policy area with key elements of integrated services. Key features of a policy area direct attention to the function of the policy area, and these are expressed through the framework of “person‐focused” and “population‐focused” services. Key elements of integrated services in turn emphasize levels of integration (macro, meso, micro level). Central questions addressed are the character of integration efforts for vulnerable groups at different stages of the life cycle and how variations therein can be understood. As a complement, sociological explanations of individual vulnerability, which are separated by causes of vulnerability into basic, conditional and triggering factors, are also used. A main finding is that the life course perspective as such does not explain variation in integration efforts; rather, it is the institutional features of the specific policy areas. These constrain or promote the potential for greater integration.