bluuebunny/crossref_metadata_2025_split huggingface dataset:
EARLY DETECTION OF RETINAL HEMANGIOBLASTOMAS IN VON HIPPEL–LINDAU DISEASE USING ULTRA-WIDEFIELD FLUORESCEIN ANGIOGRAPHY
Authors: Chen Xuejing , Sanfilippo Christian J. , Nagiel Aaron , Hosseini Hamid , Mitchell Devery , McCannel Colin A. , Schwartz Steven D. , McCannel Tara A. 
Published: March 2017

Purpose:
To study the use of ultra-widefield fluorescein angiography (UWF FA) in the detection and management of retinal capillary hemangioblastomas in patients with von Hippel–Lindau disease.

Methods:
This is a retrospective study of patients with von Hippel–Lindau disease who underwent UWF FA using the Optos camera at a single center from June 2009 to May 2015. The clinical use of UWF FA was reviewed, and the number of hemangioblastomas identified on UWF FA was compared with ophthalmoscopy and a simulated seven standard field (7SF) FA montage.

Results:
Twenty eyes of 10 patients were identified. Only 33% of lesions seen on UWF FA were also found on ophthalmoscopy, and 88% of lesions visualized on UWF FA were located outside the 7SF overlay. In 5 eyes that had gaze steering, 18% of lesions could be visualized only on gaze-steered images. For the 14 eyes with data available, 6 had procedures recommended and 8 eyes observed based on data from UWF FA. One of 20 eyes had a lesion on ophthalmoscopy that was missed by imaging.

Conclusion:
Ultra-widefield FA using the Optos camera is helpful for the evaluation and management of patients with von Hippel–Lindau disease. The UWF FA with gaze steering appears to detect more hemangioblastomas than ophthalmoscopy and conventional angiography.
Implementation strategies in pediatric neurocritical care
Authors: Markham Christopher , Proctor Enola K. , Pineda Jose A. 
Published: March 2017

Purpose of review
Brain-directed critical care for children is a relatively new area of subspecialization in pediatric critical care. Pediatric neurocritical care teams combine the expertise of neurology, neurosurgery, and critical care medicine. The positive impact of delivering specialized care to pediatric patients with acute neurological illness is becoming more apparent, but the optimum way to implement and sustain the delivery of this is complicated and poorly understood. We aim to provide emerging evidence supporting that effective implementation of pediatric neurocritical care pathways can improve patient survival and outcomes. We also provide an overview of the most effective strategies across the field of implementation science that can facilitate deployment of neurocritical care pathways in the pediatric ICU.

Recent findings
Implementation strategies can broadly be grouped according to six categories: planning, educating, restructuring, financing, managing quality, and attending to the policy context. Using a combination of these strategies in the last decade, several institutions have improved patient morbidity and mortality. Although much work remains to be done, emerging evidence supports that implementation of evidence-based care pathways for critically ill children with two common neurological diagnoses – status epilepticus and traumatic brain injury – improves outcomes.

Summary
Pediatric and neonatal neurocritical care programs that support evidence-based care can be effectively structured using appropriately sequenced implementation strategies to improve outcomes across a variety of patient populations and in a variety of healthcare settings.
The Effects of Postdischarge Telephone Counseling and Short Message Service on the Knee Function, Activities of Daily Living, and Life Satisfaction of Patients Undergoing Total Knee Replacement
Authors: Park Kyung Hye , Song Mi Ryeong 
Published: March 2017

BACKGROUND:
No study comparing short message service (SMS) texts and telephone counseling for patients undergoing total knee replacement (TKR) has been reported.

PURPOSE:
The purpose of the study was to provide postdischarge telephone counseling and SMS texts to TKR patients and to analyze the effects of these services on their knee function (KF), activities of daily living (ADL), and life satisfaction (LS).

METHODS:
This study used a randomized clinical trial design. This study was conducted with 40 patients (counseling group: 21; SMS group: 19). In the telephone counseling group and the SMS group, KF, ADL, and LS were assessed before surgery and 1 and 3 months after TKR.

RESULTS:
Telephone counseling and SMS texts have the same effects on KF, ADL, and LS of TKR patients.

CONCLUSION:
Future research is needed to determine optimal frequency and duration of post-TKR SMS to support patients who have undergone TKR.
Acute Myeloid Leukemia (AML): Upregulation of BAALC/MN1/MLLT11/EVI1 Gene Cluster Relate With Poor Overall Survival and a Possible Linkage With Coexpression of MYC/BCL2 Proteins
Authors: Akhter Ariz , Farooq Fahad , Elyamany Ghaleb , Mughal Muhammad K. , Rashid-Kolvear Fariborz , Shabani-Rad Meer-Taher , Street Lesley , Mansoor Adnan 
Published: March 2017

Background:
Molecular heterogeneity accounts for the variable and often poor prognosis in acute myeloid leukemia (AML). The current risk stratification strategy in clinical practice is limited to karyotyping and limited molecular studies screening for genetic mutations such as FLT-3 and NPM1. There is opportunity to identify further molecular prognostic markers, which may also lay the groundwork for the development of novel targeted therapies. Complex molecular technologies require transition into widely available laboratory platforms, for better integration into routine clinical practice.

Method:
In a defined subset (MYC
+
/BCL2
+
or MYC
−
/BCL2
−
) of AML patients (n=20), we examined expression signature of several genes (n=12) of established prognostic value in AML. RNA expression and MYC/BCL2 protein pattern was correlated with 3 cytogenetic risk groups and overall survival.

Results:
K-means++ unsupervised clustering defined 2 distinct groups with high and low transcript levels of BAALC/MN1/MLLT11/EVI1/SOCS2 genes (>2.5-fold difference; P<0.001). This mRNA signature trended with higher prevalence of MYC/BCL2 coexpression (P<0.057) and poor overall survival (P<0.036), but did not correlate with conventional cytogenetic risk groups (P<0.084).

Conclusions:
This pilot study provides useful data, which may help further refine the prognostic scheme of AML patients outside conventional cytogenetic risk groups. It also presents some biological rationale for future studies to explore the use of novel agents targeting MYC and/or BCL2 genes in combination with conventional chemotherapy protocols for AML.
Cellular Interplay and Cytokine Hierarchy Cause Pathological Cardiac Hypertrophy inRAF1-Mutant Noonan Syndrome
Authors: Yin Jiani C. , Platt Mathew J. , Tian Xixi , Wu Xue , Backx Peter H. , Simpson Jeremy A. , Araki Toshiyuki , Neel Benjamin G. 
Published: March 2017

AbstractNoonan syndrome (NS) is caused by mutations in RAS/ERK pathway genes, and is characterized by craniofacial, growth, cognitive and cardiac defects. NS patients with kinase-activatingRAF1alleles typically develop pathological left ventricular hypertrophy (LVH), which is reproduced inRaf1L613V/+knock-in mice. Here, using inducibleRaf1L613Vexpression, we show that LVH results from the interplay of cardiac cell types. CardiomyocyteRaf1L613Venhances Ca2+sensitivity and cardiac contractility without causing hypertrophy.Raf1L613Vexpression in cardiomyocytes or activated fibroblasts exacerbates pressure overload-evoked fibrosis. Endothelial/endocardial (EC)Raf1L613Vcauses cardiac hypertrophy without affecting contractility. Co-culture and neutralizing antibody experiments reveal a cytokine (TNF/IL6) hierarchy inRaf1L613V-expressingECs that drives cardiomyocyte hypertrophyin vitro. Furthermore, post-natal TNF inhibition normalizes the increased wall thickness and cardiomyocyte hypertrophyin vivo. We conclude that NS cardiomyopathy involves cardiomyocytes, ECs, and fibroblasts, TNF/IL6 signaling components represent potential therapeutic targets, and abnormal EC signaling might contribute to other forms of LVH.
TheToxoplasmacentrocone houses cell cycle regulatory factors
Authors: Naumov Anatoli , Kratzer Stella , Ting Li-Min , Kim Kami , Suvorova Elena S. , White Michael W. 
Published: March 2017

ABSTRACTOur knowledge of cell cycle regulatory mechanisms in apicomplexan parasites is very limited. In this study, we describe a novelToxoplasma gondiifactor, essential for chromosome replication 1 (ECR1), that has a vital role in chromosome replication and the regulation of cytoplasmic and nuclear mitotic structures. ECR1 was discovered by complementation of a temperature sensitive (ts) mutant that suffers lethal, uncontrolled chromosome replication at 40°C similar to a ts-mutant carrying a defect in topoisomerase. ECR1 is a 52kDa protein containing divergent RING and TRAF-Sina like zinc-binding domains that is dynamically expressed in the tachyzoite cell cycle. ECR1 first appears in the centrocone compartment of the nuclear envelope in early S phase and then in the nucleus in late S phase where it reaches maximum expression. Following nuclear division, but before daughters resolve from the mother, ECR1 is down regulated and is absent in new daughter parasites. The proteomics of ECR1 identified interactions with the ubiquitin-mediated protein degradation machinery and the minichromosome maintenance complex and the loss of ECR1 led to increased stability of a key member of this complex, MCM2. ECR1 also forms a stable complex with the CDK-related kinase, TgCrk5, which shares a similar cell cycle expression and localization during tachyzoite replication. Altogether, the results of this study suggest ECR1 may be a unique E3 ligase that regulates DNA licensing and other mitotic processes. Importantly, the localization of ECR1/TgCrk5 in the centrocone indicates this Apicomplexa-specific spindle compartment houses important regulatory factors that control the parasite cell cycle.IMPORTANCEParasites of the apicomplexan family are important causes of human disease including malaria, toxoplasmosis, and cryptosporidiosis. Parasite growth is the underlying cause of pathogenesis, yet despite this importance the molecular basis for parasite replication is poorly understood. Filling this knowledge gap cannot be accomplished by mining recent whole genome sequencing because apicomplexan cell cycles differ substantially and lack many of the key regulatory factors of well-studied yeast and mammalian cell division models. We have utilized forward genetics to discover essential factors that regulate cell division in these parasites using theToxoplasma gondiimodel. An example of this approach is described here with the discovery of a putative E3 ligase/protein kinase mechanism involved in regulating chromosome replication and mitotic processes of asexual stage parasites.
Horizontal Gene Transfer of Functional Type VI Killing Genes by Natural Transformation
Authors: Thomas Jacob , Watve Samit S. , Ratcliff William C. , Hammer Brian K. 
Published: March 2017

AbstractHorizontal gene transfer can have profound effects on bacterial evolution by allowing individuals to rapidly acquire adaptive traits that shape their strategies for competition. One strategy for intermicrobial antagonism often used by Proteobacteria is the genetically-encoded contact-dependent Type VI secretion system (T6SS); a weapon used to kill heteroclonal neighbors by direct injection of toxic effectors. Here, we experimentally demonstrate thatVibrio choleraecan acquire new T6SS effector genes via horizontal transfer and utilize them to kill neighboring cells. Replacement of one or more parental alleles with novel effectors allows the recombinant strain to dramatically outcompete its parent. Through spatially-explicit simulation modeling, we show that the HGT is risky: transformation brings a cell into conflict with its former clonemates, but can be adaptive when superior T6SS alleles are acquired. More generally, we find that these costs and benefits are not symmetric, and that high rates of HGT can act as hedge against competitors with unpredictable T6SS efficacy. We conclude that antagonism and horizontal transfer drive successive rounds of weapons-optimization and selective sweeps, dynamically shaping the composition of microbial communities.
Gender differences in lifetime psychiatric and substance use disorders among people who use substances in Barcelona, Spain
Authors: Frem Yasmina , Torrens Marta , Domingo-Salvany Antonia , Gilchrist Gail 
Published: March 2017

Purpose
The purpose of this paper is to examine gender differences in lifetime substance use and non-substance use (non-SUD) psychiatric disorders among illicit drug users and determine factors associated with non-SUD psychiatric disorders independently for males and for females.

Design/methodology/approach
Secondary analysis of five cross-sectional studies conducted in Barcelona, Spain during 2000-2006. Lifetime DSM-IV substance use and non-SUD psychiatric diagnoses were assessed using the Spanish Psychiatric Research Interview for Substance and Mental disorders (PRISM) among 629 people who use substances (68 per cent male) recruited from treatment (n=304) and out of treatment (n=325) settings. Odds ratios (OR) and 95% confidence intervals (CI) were calculated using binary logistic regression.

Findings
The prevalence of any lifetime psychiatric (non-SUD) disorder was 41.8 per cent, with major depression (17 per cent) and antisocial personality disorder (17 per cent) being the most prevalent disorders. After adjusting for age and study, the odds of having any lifetime non-SUD (OR 2.10; 95%CI 1.48, 2.96); any mood disorder (OR 2.13; 95%CI 1.46, 3.11); any anxiety disorder (OR 1.86; 95%CI 1.19; 2.92); any eating disorder (OR 3.09; 95%CI 1.47, 6.47); or borderline personality disorder (OR 2.30; 95%CI 1.36, 3.84) were greater for females than males. Females were less likely than males to meet criteria for antisocial personality disorder (OR 0.59; 95%CI 0.36, 0.96) and attention deficit disorder (OR 0.37; 95%CI 0.17, 0.78).

Research limitations/implications
Psychiatric disorders are common among people who use substances, with gender differences reported for specific disorders. Gender-sensitive integrated treatment approaches are required to prevent and to address comorbidity psychiatric disorders among this population.

Originality/value
This secondary analysis of five cross-sectional studies included a large sample size allowing sufficient power to examine the differences between men and women. An additional strength of the methodology is the use of the gold standard PRISM which was used to assess disorders.
Acculturation and psychological well-being of immigrant brides in Taiwan
Authors: Li Chun-Hao , Hsu Shu-Yao 
Published: March 2017

Purpose
Since the 1990s, an imbalanced marriage market has ushered in a great number of female immigrants, mainly from China and certain ASEAN countries, into Taiwan. These immigrant brides lose their original social networks and face difficulties acculturating, which in the long run may take a toll on their psychological well-being. The purpose of this paper is to identify what the factors are and how they are associated with the symptoms of depression and anxiety among immigrant brides.

Design/methodology/approach
Due to the difficulty of identifying immigrant brides within the general population, the research relies on a convenience sample of 366 immigrant brides in eight cities or counties in Taiwan. The research team provided questionnaires in different languages including Traditional Chinese, Simplified Chinese, English, Vietnamese, Thai, and Indonesian. Surveyed immigrant brides filled in questionnaires while participating in activities for Immigrant Day.

Findings
Hierarchical regression analyses show that perceived discrimination and sociocultural barriers are the two greatest factors causing depression and anxiety among immigrant brides. However, the effect of social support is not as clear as expected. Emotional social support from “the same ethnic group” is significant in reducing the symptoms of psychological depression of immigrant brides, but it cannot explain the variation in anxiety among immigrant brides.

Originality/value
Although the psychological well-being of immigrant brides in Taiwan is an important issue, little scholarly research has been published on this increasing segment of the population. The research can improve our understanding of the acculturation of immigrant brides in Taiwan. Meanwhile, the findings in the Taiwan-based research may provide further helpful knowledge for South Korea and China, both of which generally share a similar culture with Taiwan and are also facing the issues of marriage market imbalance and the increase of immigrant brides, in developing prevention and intervention strategies and/or programs to enhance the psychological well-being of immigrant brides.
Patterns of living arrangements of the elderly in mainland China
Authors: Lu Jiehua , Zhang Yun 
Published: March 2017

Purpose
The purpose of this paper is to examine the changes and consequences of the patterns of Chinese elderly population’s living arrangements. It contains information that can be considered for future policy making for the elderly and to gain a better understanding of the social transition in China.

Design/methodology/approach
Based on the analysis of the population census data in 2000 and 2010, the authors examined the changes and trends of the living arrangements of the elderly Chinese population. Furthermore, the authors analyzed factors influencing the Chinese elderly’s living arrangements according to the data acquired from the Chinese Longitudinal Healthy Longevity Survey.

Findings
First, the proportion of the elderly people living with children has been decreasing. Second, the proportions of elderly people “living alone” and “living with spouse independently” has largely increased. Third, the changes and trends showed differences between urban and rural regions.

Originality/value
By looking at the characteristics among elderly people with different living arrangement patterns, those that are “living alone” are typically in disadvantaged conditions, and thus special attention should be paid with regards to related research and policies for the elderly who are “living alone.”
What if people’s private life constrained their career decisions? Examining the relationship between home-to-career interference and career satisfaction
Authors: Schooreel Tess , Shockley Kristen Michelle , Verbruggen Marijke 
Published: March 2017

PurposePrevious research suggests that employees often make family-related career decisions (Greenhaus and Powell, 2012). The authors extend this idea and explore the concept of “home-to-career interference,” defined as the extent to which people perceive that their private life has constrained their career decisions to date. The authors expect that home-to-career interference has a negative impact on employees’ later career satisfaction via career goal self-efficacy and perceived organizational career support. The paper aims to discuss these issues.Design/methodology/approachThe authors collected quantitative data at three points in time, each six months apart in a Belgian telecommunications organization. Using the full information maximum likelihood path analysis approach, the authors performed analyses on a sample of dual-earner employees.FindingsThe results showed that employees’ home-to-career interference related negatively to their career goal self-efficacy and perceived organizational career support, which were, in turn, positively related to their career satisfaction.Originality/valueThis study contributes to the work-family literature by introducing the concept of home-to-career interference, by clarifying the mechanisms through which home-to-career interference relates to career satisfaction and by testing these relationships using a three-wave longitudinal design.
Becoming a transformative vice-principal in culturally and linguistically rich diverse schools
Authors: Hamm Lyle 
Published: March 2017

Purpose
The purpose of this paper is to examine the leadership role(s) of vice-principals in diverse, multi-ethnic schools and communities and understand the supervision and mentoring support they require to help them become more effective leaders within them. The research questions guiding this study were: what forms of mentoring do vice-principals, who serve in diverse schools in rapidly changing communities, require? Who is in the best position to provide mentoring for them?

Design/methodology/approach
The author used a qualitative case study methodology. Data that were analyzed for this paper were drawn from surveys, semi-structured interviews, one focus group interview and school and community documents from three data sets within two case studies in Canada. The first data set was part of the author’s doctoral research program in a diverse school in Alberta; the other case study was part of a larger collective case study that the author is currently involved with and leading in New Brunswick. Several vice-principals were part of both studies. The author then constructed a survey questionnaire specifically focused on mentoring vice-principals in diverse schools. Vice-principals in both provinces, who were part of the two studies, were invited to respond to the follow-up survey. Using a constant comparative analytical approach, the author coded and analyzed the data from all three sets together. The author formed several categories and ultimately collapsed the categories into five distinct themes that illustrated and confirmed the social realities of the vice-principals in their schools and communities.

Findings
Five key findings emerged from the analysis of the data sets. They were building leadership capacity, fostering positive relationships, increasing global awareness, reducing stress and anxiety and becoming a diversity champion and peace-builder.

Originality/value
To this researcher’s knowledge, this paper contributes to a significant gap in the literature on vice-principals who serve in diverse schools and communities.