bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between serum insulin-like growth factor-1 and bone mineral density in patients with type 2 diabetes
Authors: Zhao Qianqian , Li Youqian , Zhang Qiuping , Zhang Mei , Ban Bo 
Published: September 2024

BackgroundSecondary osteoporosis is associated with type 2 diabetes mellitus (T2DM), and there is conflicting evidence regarding the relationship between insulin-like growth factor-1 (IGF-1) and bone mineral density (BMD) in different populations. The objective of this study was to investigate the relationship between serum IGF-1 levels and BMD in patients with T2DM.MethodA retrospective cross-sectional study was performed on a cohort of 363 patients with T2DM, comprising men aged over 50 and women who are postmenopausal. Those with no significant medical history or medication affecting BMD or IGF-1 were considered. Data analyzed included IGF-1 levels, markers of bone metabolism, and measurements of BMD. To account for age and gender variations, we calculated IGF-1 standard deviation scores (IGF-1 SDS) for further investigation.ResultsA significant increase in BMD at lumbar spine (LS), femoral neck (FN), and total hip (TH) was observed as IGF-1 SDS tertiles rose. We revealed a nonlinear correlation between IGF-1 SDS and BMD at these sites, with a common inflection point identified at an IGF-1 SDS level of -1.68. Additionally, our multivariate piecewise linear regression analysis highlighted a positive association between IGF-1 SDS and BMD at LS, FN, and TH when IGF-1 SDS exceeded the inflection point (β 0.02, 95% CI 0.01, 0.04 for LS; β 0.02, 95% CI 0.01, 0.03 for FN; β 0.02, 95% CI 0.01, 0.03 for TH). Conversely, below the inflection point, this association was not significant (β -0.04, 95% CI -0.10, 0.01 for LS; β -0.04, 95% CI -0.08, 0.01 for FN; β -0.03, 95% CI -0.08, 0.01 for TH).ConclusionThese findings reveal a nonlinear relationship between IGF-1 SDS and BMD in T2DM patients. Higher serum IGF-1 levels were connected to increased bone density only after surpassing a certain threshold.
Role of disulfidptosis in colorectal adenocarcinoma: implications for prognosis and immunity
Authors: Yang Ruanruan , Lai Chunxiao , Huang Luji , Li Feng , Peng Weiqi , Wu Meiyan , Xin Jinge , Lu Yan , Ouyang Manzhao , Bai Yang , Lei Haoqiang , He Shunhui , Lin Yu 
Published: September 2024

BackgroundRecent research has found a new way of cell death: disulfidptosis. Under glucose starvation, abnormal accumulation of disulfide molecules such as Cystine in Solute Carrier Family 7 Member 11 (SLC7A11) overexpression cells induced disulfide stress to trigger cell death. The research on disulfidptosis is still in its early stages, and its role in the occurrence and development of colorectal malignancies is still unclear.MethodIn this study, we employed bioinformatics methods to analyze the expression and mutation characteristics of disulfidptosis-related genes (DRGs) in colorectal cancer. Consensus clustering analysis was used to identify molecular subtypes of Colorectal Adenocarcinoma (COAD) associated with disulfidptosis. The biological behaviors between subtypes were analyzed to explore the impact of disulfidptosis on the tumor microenvironment. Constructing and validating a prognostic risk model for COAD using diverse data. The influence of key genes on prognosis was evaluated through SHapley Additive exPlanations (SHAP) analysis, and the predictive capability of the model was assessed using Overall Survival analysis, Area Under Curve and risk curves. The immunological status of different patients and the prediction of drug treatment response were determined through immune cell infiltration, TMB, MSI status, and drug sensitivity analysis. Single-cell analysis was employed to explore the expression of genes at the cellular level, and finally validated the expression of key genes in clinical samples.ResultBy integrating the public data from two platforms, we identified 2 colorectal cancer subtypes related to DRGs. Ultimately, we established a prognosis risk model for COAD using 7 genes (FABA4+GIPC2+EGR3+HOXC6+CCL11+CXCL10+ITLN1). SHAP analysis can further explained the positive or negative impact of gene expression on prognosis. By dividing patients into high-risk and low-risk groups, we found that patients in the high-risk group had poorer prognosis, higher TMB, and a higher proportion of MSI-H and MSI-L statuses. We also predicted that drugs such as 5-Fluorouracil, Oxaliplatin, Gefitinib, and Sorafenib would be more effective in low-risk patients, while drugs like Luminesib and Staurosporine would be more effective in high-risk patients. Single-cell analysis revealed that these 7 genes not only differ at the level of immune cells but also in epithelial cells, fibroblasts, and myofibroblasts, among other cell types. Finally, the expression of these key genes was verified in clinical samples, with consistent results.ConclusionsOur research findings provide evidence for the role of disulfidptosis in COAD and offer new insights for personalized and precise treatment of COAD.
The pathological significance and potential mechanism of ACLY in cholangiocarcinoma
Authors: Sun Xiaoyan , Zhao Xiaofang , Wang Senyan , Liu Qi , Wei Wenjuan , Xu Jing , Wang Hongyang , Yang Wen 
Published: September 2024

Background and aimCholangiocarcinoma (CCA) is a rare cancer, yet its incidence and mortality rates have been steadily increasing globally over the past few decades. Currently, there are no effective targeted treatment strategies available for patients. ACLY (ATP Citrate Lyase), a key enzyme in de novo lipogenesis, is aberrantly expressed in several tumors and is associated with malignant progression. However, its role and mechanisms in CCA have not yet been elucidated.MethodsThe expression of ACLY in CCA was assessed using transcriptomic profiles and tissue microarrays. Kaplan-Meier curves were employed to evaluate the prognostic significance of ACLY in CCA. Functional enrichment analysis was used to explore the potential mechanisms of ACLY in CCA. A series of assays were conducted to examine the effects of ACLY on the proliferation and migration of CCA cells. Ferroptosis inducers and inhibitors, along with lipid peroxide probes and MDA assay kits, were utilized to explore the role of ACLY in ferroptosis within CCA. Additionally, lipid-depleted fetal bovine serum and several fatty acids were used to evaluate the impact of fatty acids on ferroptosis induced by ACLY inhibition. Correlation analyses were performed to elucidate the relationship between ACLY and tumor stemness as well as tumor microenvironment.ResultsThe expression of ACLY was found to be higher in CCA tissues compared to adjacent normal tissues. Patients with elevated ACLY expression demonstrated poorer overall survival outcomes. ACLY were closed associated with fatty acid metabolism and tumor-initiating cells. Knockdown of ACLY did not significantly impact the proliferation and migration of CCA cells. However, ACLY inhibition led to increased accumulation of lipid peroxides and enhanced sensitivity of CCA cells to ferroptosis inducers. Polyunsaturated fatty acids were observed to inhibit the proliferation of ACLY-knockdown cells; nonetheless, this inhibitory effect was diminished when the cells were cultured in medium supplemented with lipid-depleted fetal bovine serum. Additionally, ACLY expression was negatively correlated with immune cell infiltration and immune scores in CCA.ConclusionACLY promotes ferroptosis by disrupting the balance of saturated and unsaturated fatty acids. ACLY may therefore serve as a potential diagnostic and therapeutic target for CCA.
Risk factors for multidrug-resistant tuberculosis: a predictive model study
Authors: Wu Lianpeng , Cai Xiaoxiao , Jiang Xiangao 
Published: September 2024

ObjectiveTo investigate the risk factors associated with Multidrug-resistant tuberculosis (MDR-TB) in people with drug-resistant tuberculosis (DR-TB) and develop a predictive model.MethodsA total of 893 individuals with DR-TB treated at Wenzhou Central Hospital from January 2018 to December 2022 were included in the study after excluding 178 individuals with incomplete clinical and laboratory data, leaving 715 individuals for analysis. Data on demographic information, baseline clinical characteristics, laboratory and imaging results, and clinical diagnosis were collected to identify the risk factors for MDR-TB and establish a predictive model.ResultsMultivariate logistic regression analysis identified residence in rural areas, retreatment of TB, presence of pulmonary cavity, uric acid (UA) ≥ 346 μmol/L and c-reactive protein (CRP) &lt; 37.3 mg/L as independent risk factors for MDR-TB in individuals with DR-TB. A nomogram model was constructed using these five factors to predict the risk of MDR-TB, with an area under the ROC curve (AUC) of 0.758 for the training group and 0.775 for the validation group. Calibration curve analysis showed good agreement between predicted and actual MDR-TB incidence in both groups, and decision curve analysis showed that the nomogram model had a higher rate of clinical net benefit.ConclusionThis study suggests that residence, types of TB treatment, presence of pulmonary cavity, UA and CRP are associated with MDR-TB occurrence in individuals with DR-TB, and the nomogram model developed in this study shows promising predictive value.
Prevalence of birth before arrival and associated factors among postpartum women in southern Ethiopia: a community-based cross-sectional study
Authors: Amanuel Tsedeke , Desalegn Mitiku , Lukas Kaleegziabher , Yohannes Tadele 
Published: September 2024

BackgroundBirth before arrival (BBA) constitutes a high-risk newborn population with high perinatal morbidity and mortality. In Ethiopia, most studies and health surveys consider only home and hospital deliveries but do not consider deliveries that take place between the house and health facility. The aim of this study was to assess the prevalence of BBA and its associated factors among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, 2023.MethodsA community-based cross-sectional study was conducted among postpartum women in Lemo woreda, Hadiya Zone, SNNPR, Ethiopia, from April 5 to May 20, 2023. Three hundred eighty-two postpartum women who gave birth 6 months prior to this study were included. Twelve out of 36 kebeles were selected randomly, and simple random sampling was employed for the selection of participant women. An interviewer-administered questionnaire was used for data collection. A binary logistic regression analysis was computed, and variables with a p value of &lt;0.25 were included in the final multivariable logistic regression analysis. Model fitness was checked via the Hosmer–Lemeshow goodness-of-fit test (x2 = 16.04, p value = 0.250). Statistical significance was declared via odds ratios and 95% confidence intervals at a p value &lt;0.05.ResultsThe prevalence of BBA among women who gave birth in the last 6 months preceding this study in the study area was 15.2% (95% CI: 11.8, 19.1%). In the multivariable analysis, the variables associated with birth before arrival in the final model were having no antenatal care (AOR = 2.63; 95% CI: 1.23, 5.63), having a female autonomy status (AOR = 3.32; 95% CI: 1.12, 9.89), not being knowledgeable about labor symptoms (AOR = 2.15; 95% CI: 1.11, 4.18), and having birth preparedness toward the index birth (AOR = 0.13; 95% CI: 0.05, 0.35).ConclusionThe prevalence of BBA in the study area was unacceptably high. A statistically significant association was observed between birth before arrival and having no antenatal care, dependent women’s autonomy status, being not knowledgeable about labor symptoms, and having birth preparedness toward the index birth.
Assessment of corneal epithelial thickness mapping by spectral-domain optical coherence tomography
Authors: Tañá-Rivero Pedro , Orts-Vila Paz , Tañá-Sanz Pedro , Ramos-Alzamora María , Montés-Micó Robert 
Published: September 2024

BackgroundTo assess corneal epithelial-thickness (ET) mapping resulting from spectral-domain-optical-coherence-tomography (SD-OCT) by analysing its repeatability and reproducibility and its utility for screening corneal-refractive-surgery (CRS) candidates.MethodsET was measured in 25-sectors by two-operators. Intra-subject-standard-deviation, coefficient-of-repeatability (CoR) and coefficient-of-variability (CoV) were calculated to evaluate repeatability. Reproducibility was evaluated using a Bland–Altman analysis. Scheimpflug-tomography, refraction, visual acuity, and patient history were used to make a decision on eligibility for CRS. After this decision, the surgeon was shown the patient’s ET map and was asked to reconsider his analysis. The percentage of screenings that changed after evaluating the ET maps was determined.ResultsForty-three eyes with normal corneas (CRS-group) and 21 eyes not suitable for CRS (non-CRS-group) were studied. For the CRS-group, CoR ranged from 2.03 (central) to 19.73 μm (outer-inferonasal), with the central-sector showing the highest repeatability (CoV: 1.53–1.80%). For the non-CRS-group, CoR ranged from 3.82 (central-middle-superonasal) to 13.42 μm (middle-inferotemporal), with the inner-superonasal-sector showing the highest repeatability (CoV: 2.86–4.46%). There was no statistically significant difference between operators (p &gt; 0.01). In the CRS-group, the outcomes showed a narrow 95% limits-of-agreement (LoA) for the central-and inner-nasal-sectors (about 4 μm), and wider for the inner-superior, outer-superotemporal and outer-inferonasal (about 10–14 μm). In the non-CRS-group, they were for the outer superonasal (about 4 μm), and for the middle-inferotemporal and outer-temporal (about 10 μm), respectively. Candidacy for CRS changed in 7.82% of patients after evaluation of the ET maps, with all of them screened-out.ConclusionThe SD-OCT provided repeatable and reproducible corneal ET measurements and may alter candidacy for CRS.Clinical trial registrationGerman Clinical Trials Register: https://drks.de/search/en/trial/DRKS00032797, identifier: DRKS00032797.
The characteristics of intestinal microbiota in patients with type 2 diabetes and the correlation with the percentage of T-helper cells
Authors: Yang Fan , Li Jinyan , Wei Longqin , Qin Shenghua , Shi Qingfeng , Lu Siyan , Chu Shuyuan 
Published: September 2024

BackgroundType 2 diabetes (T2D) is related to intestinal microflora changes and immune inflammation. We aimed to investigate the pattern of intestinal flora-systematic T helper (Th) cell linkage in T2D patients.MethodsParticipants with T2D diagnosed by physicians and healthy controls were enrolled in the study. The Th1, Th2, and Th17 cells from the peripheral blood were assessed by flow cytometry. The feces were collected. The V3–V4 variable region of 16S rRNA was sequenced and analyzed using bioinformatics. Principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis were performed to assess the beta diversity. The linear discriminant analysis (LDA) effect size (LEfSe) method was applied to identify amicrobial taxon specific to T2D. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was conducted to identify the metabolic pathways. A network analysis was conducted by constructing a co-occurrence network.ResultsThe percentages of the Th1 and Th17 cells in the peripheral blood were higher in patients with T2D than in controls. Among the top 30 genera of the intestinal microbiota, the levels of Lachnospiraceae_NK4A136_group, Ruminococcaceae_UCG002, and Eubacterium_hallii_group were lower in the patients with T2D than in controls. In the LEfSe analysis, it was observed that the Lachnospiraceae and Ruminococcaceae families were significantly different between patients with T2D and controls. Moreover, the Th1/Th2 ratio was positively correlated with the abundance of the Lachnoclostridium and Ruminococcus_torques_group genera. In the network analysis, the Th1/Th2 ratio, Ruminococcaceae_UCG-002, and Lachnospiraceae_NK4A136_group were the important nodes.ConclusionThis study provided a preliminary picture of the crosstalk between the intestinal microbiome and systematic Th cells in patients with T2D. The findings of the study suggested that the network relationship among the intestinal microbiota, metabolites, and CD4+T lymphocyte immunity was unbalanced in the patients with T2D, which might have promoted the development of T2D. This presents a therapeutic opportunity to modulate gut immune reaction and then chronic inflammation by manipulating microbiome-specific Th-cell response.
PXDN as a pan-cancer biomarker and promotes tumor progress via immune inhibition in nasopharyngeal carcinoma
Authors: Li Yu-Chun , Xiao Yan-Hong , Chen Fei-Xiong , Xiao Si-Yu , Lin Jian-Mei , Cai Shu-Tao , Zeng Cui-Lan , Ye Xue-Yan , Yu Xu-Fa , Yuan Li , Li Shi-Bing 
Published: September 2024

BackgroundInvestigating oncogenes and the mechanisms driving oncogenic processes in human tumors is imperative for the development of efficient therapies. Peroxidasin (PXDN) has been reported to play a critical role in tissue development and homeostasis. However, the role of PXDN in the occurrence and development of Nasopharyngeal Carcinoma (NPC) remains unknown.MethodsData from multiple databases, including GEO and TCGA, were used to analyze the expression levels of PXDN. Taking nasopharyngeal carcinoma as an example, in vitro experiments were conducted to explore the biological functions of PXDN. Overexpression of stable cell lines was achieved through lentiviral infection, cell proliferation was examined using CCK8 and BrdU incorporation assays, and clone formation experiments were performed to assess cell growth. Transwell and wound healing assays were employed to evaluate cell invasion and migration abilities. Additionally, immunofluorescence staining with multiple targets was used to analyze the immune microenvironment of the tumor tissues. Co-culture experiments, followed by clone formation and CFSE incorporation assays, were conducted to observe the impact of NPC stable cell lines on T cells. Flow cytometry was performed to detect surface markers and cytokines in T cells after co-culture to assess T cell function.ResultsPXDN was highly expressed in multiple tumors, and its high expression and mutation profile were correlated with poor survival. Functionally, PXDN plays a crucial role in promoting oncogenic processes by enhancing NPC cell proliferation and metastasis. Mechanistically, PXDN activates extracellular matrix (ECM) signaling pathways while simultaneously inhibiting T-cell infiltration and activation, thereby facilitating cancer progression.ConclusionWe characterized PXDN as a valuable biomarker for pan-cancer diagnosis and prognosis. We also uncovered new oncogenic roles for PXDN in promoting cancer progression and regulating T-cell immunosuppressive function in NPC.
A systematic review and meta-analysis of intraperitoneal anastomosis versus extraperitoneal anastomosis in laparoscopic left colectomy
Authors: Zhou Wenjie , Wang Xueting , Dan Jie , Zhu Mingjie , Li Ming , Liu Ke , Liao Qian , Wang Yonghong 
Published: September 2024

BackgroundThe effectiveness of the anastomosis method for laparoscopic left colectomy (LLC) remains inconclusive. Thus, a systematic review and meta-analysis were conducted to compare the outcomes between intraperitoneal anastomosis (IPA) and extraperitoneal anastomosis(EPA)in LLC.MethodsPubMed, Embase, the Cochrane Library, CNKI, and WanFangData were systematically searched for relevant literature. The literature was screened independently by two groups, and data were extracted and evaluated for bias. Meta-analysis was performed using Revman5.4 software.ResultsTwelve studies with a total of 1,278 patients were included in our meta-analysis. Compared with the EPA group, the IPA group had less blood loss [odds ratio (OR)=–20.32, 95% confidence interval (CI) (−27.98–12.65), p&lt;0.00001], a lower overall complication rate [OR=0.45, 95% CI (0.33–0.63), p&lt;0.00001], fewer non-severe complications [OR=0.44, 95% CI (0.30–0.64), p&lt;0.0001], and fewer surgical site infections [OR=0.39, 95% CI (0.21–0.71), p=0.002]. Additionally, a longer operation time appeared in the multicenter and propensity score matching (PSM) subgroups of the IPA group. Furthermore, patients in the IPA group had an earlier exhaust time and shorter hospital stays. There were no significant differences between the two groups regarding severe complications, anastomose-related complications, postoperative blood transfusion, ileus, reoperation rate, time to stool, pathologic sample length, and lymph node dissection number.ConclusionIPA seems more advantageous than EPA for patients receiving LCC in terms of complications and postoperative recovery and has similar oncological outcomes. However, it may take longer and be more difficult to perform.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/#recordDetails PROSPERO, identifier (CRD4202454391).
Anisodamine (654-1/654-2) ameliorates septic kidney injury in rats by inhibiting inflammation and apoptosis
Authors: Liu Dong , Tang Fei , Zhang Li , Wan Feng , Xu Li-Yue , Zhang Jing-Nan , Zhao Xiao-Lan , Ao Hui , Peng Cheng 
Published: September 2024

IntroductionTo investigate the protective effects of anisodamine (654-1/654-2) against acute kidney injury (AKI) in LPS-induced septic shock rats and explore its molecular mechanisms.Methods56 rats were randomly divided into 8 groups: control, LPS, LPS + 654-1, and LPS + 654-2 (1.25, 2.5 and 5 mg/kg). The model was evaluated by monitoring MAP, HR, and plasma LD levels. ELISA and biochemical assay kits were used to measure the levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and kidney injury markers (BUN and CRE). Additionally, RNA-seq and bioinformatic analysis were performed to explore the mechanism of action of 654-1/654-2, and verification was conducted by western blotting and RT-PCR.Results654-1/654-2 significantly restored the levels of MAP, HR, and plasma LD in septic shock rats. Furthermore, 654-1/654-2 (5 mg/kg) effectively ameliorated LPS-induced kidney structural damage and exhibited a dose-dependent reduction in levels of inflammatory cytokines and kidney injury markers. In addition, RNA-seq, WB, and RT-PCR analyses revealed that 654-1/654-2 exerted its effects by inhibiting the expressions of the NF-κB and MAPK pathways and activating the Pi3K/Akt/Bcl-2 signaling pathway, thereby mitigating AKI.DiscussionThis study suggested that 654-1/654-2 could alleviate AKI in septic shock rats by improving inflammation invasion and cell apoptosis. Notably, 654-1/654-2 collectively suppressed inflammation response through the p38/JNK/AP-1/NF-κB pathway. Additionally, 654-1 promotes survival signaling via the Pi3K/Akt/Bcl-2 pathway, whereas 654-2 reduces apoptosis through the P53/Bax pathway. These findings provided a theoretical basis for the clinical application of 654-1/654-2 in treating organ damage caused by septic shock.