bluuebunny/crossref_metadata_2025_split huggingface dataset:
PALLD Regulates Phagocytosis by Enabling Timely Actin Polymerization and Depolymerization
Authors: Sun Hai-Min , Chen Xin-Lei , Chen Xin-Jie , Liu Jin , Ma Lie , Wu Hai-Yan , Huang Qiu-Hua , Xi Xiao-Dong , Yin Tong , Zhu Jiang , Chen Zhu , Chen Sai-Juan 
Published: July 2017

Abstract
PALLD is an actin cross-linker supporting cellular mechanical tension. However, its involvement in the regulation of phagocytosis, a cellular activity essential for innate immunity and physiological tissue turnover, is unclear. We report that PALLD is highly induced along with all-trans-retinoic acid–induced maturation of myeloid leukemia cells, to promote Ig- or complement-opsonized phagocytosis. PALLD mechanistically facilitates phagocytic receptor clustering by regulating actin polymerization and c-Src dynamic activation during particle binding and early phagosome formation. PALLD is also required at the nascent phagosome to recruit phosphatase oculocerebrorenal syndrome of Lowe, which regulates phosphatidylinositol-4,5-bisphosphate hydrolysis and actin depolymerization to complete phagosome closure. Collectively, our results show a new function for PALLD as a crucial regulator of the early phase of phagocytosis by elaborating dynamic actin polymerization and depolymerization.
Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance
Authors: Strandt Helen , Pinheiro Douglas Florindo , Kaplan Daniel H. , Wirth Dagmar , Gratz Iris Karina , Hammerl Peter , Thalhamer Josef , Stoecklinger Angelika 
Published: July 2017

Abstract
The skin hosts a variety of dendritic cells (DCs), which act as professional APC to control cutaneous immunity. Langerhans cells (LCs) are the only DC subset in the healthy epidermis. However, due to the complexity of the skin DC network, their relative contribution to either immune activation or immune tolerance is still not entirely understood. To specifically study the function of LCs in vivo, without altering the DC subset composition in the skin, we have generated transgenic mouse models for tamoxifen-inducible de novo expression of Ags in LCs but no other langerin+ DCs. Therefore, this system allows for LC-restricted Ag presentation to T cells. Presentation of nonsecreted OVA (GFPOVA) by steady-state LCs resulted in transient activation of endogenous CTL in transgenic mice. However, when these mice were challenged with OVA by gene gun immunization in the contraction phase of the primary CTL response they did not respond with a recall of CTL memory but, instead, with robust Ag-specific CTL tolerance. We found regulatory T cells (Tregs) enriched in the skin of tolerized mice, and depletion of Tregs or adoptive experiments revealed that Tregs were critically involved in CTL tolerance. By contrast, when OVA was presented by activated LCs, a recallable CTL memory response developed in transgenic mice. Thus, neoantigen presentation by epidermal LCs results in either robust CTL tolerance or CTL memory, and this decision-making depends on the activation state of the presenting LCs.
Cutting Edge: TRAF6 Mediates TLR/IL-1R Signaling–Induced Nontranscriptional Priming of the NLRP3 Inflammasome
Authors: Xing Yue , Yao Xiaomin , Li Hua , Xue Guang , Guo Qiuhong , Yang Guiwen , An Liguo , Zhang Yan , Meng Guangxun 
Published: July 2017

Abstract
NLRP3 inflammasome activiation requires two sequential signals. The priming signal 1 from TLRs or cytokine receptors induces the transcription of NLRP3 and IL-1β, and concomitantly promotes transcription-independent activation of caspase-1. The activating signal 2 can be provided by microbial products or danger signals. In this study we found that TRAF6 is necessary for the nontranscriptional priming of NLRP3 inflammasome by TLR/IL-1R derived signals. Deficiency of TRAF6 specifically inhibited TLR/IL-1R priming–initiated caspase-1 cleavage, pyroptosis, and secretion of presynthesized IL-18. Mechanistically, TRAF6 promoted NLRP3 oligomerization as well as the interaction between NLRP3 and apoptosis-associated speck-like protein containing a CARD. Of note, the nontranscriptional priming via TRAF6 did not involve mitochondrial reactive oxygen species or the phosphorylation of Jnk, Erk, and Syk, whereas the ubiquitin E3 ligase activity of TRAF6 was required. Our findings thus extended cognition on the mechanism of NLRP3 inflammasome activation, and provided a novel target for controlling NLRP3-related diseases.
Trypanosoma musculi Infection in Mice Critically Relies on Mannose Receptor–Mediated Arginase Induction by a TbKHC1 Kinesin H Chain Homolog
Authors: Nzoumbou-Boko Romaric , De Muylder Géraldine , Semballa Silla , Lecordier Laurence , Dauchy Fréderic-Antoine , Gobert Alain P. , Holzmuller Philippe , Lemesre Jean-Loup , Bras-Gonçalves Rachel , Barnabé Christian , Courtois Pierrette , Daulouède Sylvie , Beschin Alain , Pays Etienne , Vincendeau Philippe 
Published: July 2017

Abstract
Arginase activity induction in macrophages is an escape mechanism developed by parasites to cope with the host’s immune defense and benefit from increased host-derived growth factor production. We report that arginase expression and activity were induced in macrophages during mouse infection by Trypanosoma musculi, a natural parasite of this host. This induction was reproduced in vitro by excreted/secreted factors of the parasite. A mAb directed to TbKHC1, an orphan kinesin H chain from Trypanosoma brucei, inhibited T. musculi excreted/secreted factor–mediated arginase induction. Anti-TbKHC1 Ab also inhibited T. musculi growth, both in vitro and in vivo. Induction of arginase activity and parasite growth involved C-type lectin receptors, because mannose injection decreased arginase activity induction and parasite load in vitro and in vivo. Accordingly, the parasite load was reduced in mice lacking mannose receptor C-type 1. The T. musculi KHC1 homolog showed high similarity with TbKHC1. Bioinformatics analysis revealed the presence of homologs of this gene in other trypanosomes, including pathogens for humans and animals. Host metabolism dysregulation represents an effective parasite mechanism to hamper the host immune response and modify host molecule production to favor parasite invasion and growth. Thus, this orphan kinesin plays an important role in promoting trypanosome infection, and its neutralization or the lock of its partner host molecules offers promising approaches to increasing resistance to infection and new developments in vaccination against trypanosomiasis.
Metodologías de evaluación de la transparencia: procedimientos y problemas
Authors: Molina Rodríguez-Navas Pedro , Simelio Solà Núria , Corcoy Rius Marta 
Published: July 2017

La aprobación de la Ley de Transparencia en España en 2013, junto a la preocupación por la corrupción, el buen gobierno y la rendición de cuentas, ha provocado un importante aumento de los estudios sobre transparencia de las administraciones en los últimos años. Metodología: El objetivo de este artículo es exponer las diferentes complejidades metodológicas para analizar la transparencia de los contenidos publicados por las administraciones públicas en aspectos como la propia definición de transparencia, el objeto de estudio, los usuarios o los destinatarios. Resultados: Se presenta un modelo de análisis de la transparencia de las webs de los ayuntamientos: el proyecto Infoparticipa. La metodología que presentamos está basada en herramientas innovadoras que han establecido un mapa de la transparencia local en España. Discusión y conclusiones: El Mapa Infoparticipa ha transcendido el ámbito académico mostrando su utilidad social al impulsar la mejora de la información publicada en las webs.
El uso de Facebook como herramienta narrativa para articular el nuevo diálogo político. Estudio de caso de la II Asamblea Ciudadana de Podemos, “Vistalegre II”
Authors: Rodríguez Fidalgo Mª Isabel , Ruiz Paz Yanira , Paíno Ambrosio Adriana , Jiménez Iglesias Lucía 
Published: July 2017

Las redes sociales se están utilizando como herramientas para trasladar el debate político con la finalidad de potenciar la interacción-participación entre el binomio ‘actores políticos-sociedad’. El presente paper centra su objetivo en el análisis de la construcción de dichas narrativas políticas generadas en la red social Facebook desde la perspectiva del concepto de “compromiso 2.0”. Metodología: Bajo una perspectiva metodológica cuantitativa y cualitativa, se exploran los nuevos relatos de participación política partiendo de los discursos en Facebook de Pablo Iglesias e Íñigo Errejón durante la II Asamblea Ciudadana de Podemos, “Vistalegre 2”, y de las reacciones ciudadanas manifestadas a través de sus comentarios. Resultados y conclusiones: De este estudio se desprende la idea del surgimiento de una narrativa política donde el ciudadano, además de cambiar su rol tradicional de emisor, potencia nuevos flujos de información bajo la fórmula receptor-emisor respectivamente convirtiéndose en el verdadero protagonista ante estas nuevas narrativas digitales políticas.
Adultilização na infância: as representações das crianças dos anos iniciais do ensino fundamental
Authors: Caetano Maria Raquel 
Published: July 2017

http://dx.doi.org/10.5007/1980-4512.2016v18n33p83O artigo faz parte de uma pesquisa realizada em uma escola e busca identificar, compreender e analisar as representações das crianças que frequentam o segundo e quarto ano do ensino fundamental frente à temática “adultilização da infância” tendo em vista que se vive no século XXI, momento no qual muitas mudanças vêm ocorrendo, entre elas mudanças sociais, políticas e econômicas, que interferem diretamente no âmbito educacional e, consequentemente, na formação de alunos e professores para este século. O estudo buscou responder em sua narrativa a seguinte questão: quais são as representações das crianças no ambiente escolar frente à adultilização da infância? Para responder a esta questão foram realizados grupos focais com as crianças, no intuito de coletar dados acerca do problema pesquisado. O estudo suscitou a compreensão de que as crianças já conseguem dizer e classificar o que é apropriado para elas e para os adultos.
A diversidade tipológica na composição de palavras neoclássicas agro-X
Authors: Silva Neide Higino da 
Published: July 2017

http://dx.doi.org/10.5007/1984-8412.2017v14n1p1779Este trabalho investiga as relações estruturais e semânticas dos compostos neoclássicos. O objetivo é identificar características que possam estabelecer vínculos entre construções que, a princípio, apresentam-se como díspares, em função dos diferentes elementos que as constituem (agrologia, agromoda, autódromo, cachorródromo) e pelos vários significados que um mesmo radical pode assumir (agro- pode significar campo em agrologia, produtos agrícolas em agroexportação e ciências agrárias em agroveterinária). No entanto, essas composições apresentam um grau de semelhança relacional que influencia a categorização dessas construções. Os dados examinados foram selecionados do autor (2016). A fundamentação teórica que norteia a análise é o continuum composição-derivação, como proposto por Bauer (2005), Kastovsky (2009), Gonçalves (2011b) e Gonçalves e Andrade (2012). Considerando uma rede de associações estruturais, de acordo com Bybee (2010), é formulado um esquema geral para os compostos neoclássicos.
Cópula invariável em clivadas invertidas: um exemplo de gramaticalização?
Authors: Silveira Damaris Matias 
Published: July 2017

http://dx.doi.org/10.5007/1984-8420.2016v17n1p30Esta pesquisa busca investigar diacronicamente as sentenças sintaticamente utilizadas para focalizar constituintes, as clivadas canônicas e invertidas. Tais sentenças caracterizam-se pelas sequências Cópula + Foco + que + IP e Foco + cópula + que + IP. O período considerado para a busca dessas sentenças é o que se estende do século XVI ao XIX, textos que compõem o Corpus Histórico do Português Tycho Brahe. Tento apre­sentar uma discussão em torno da gramaticalização da cópula das clivadas invertidas atra­vés da maior evidência para o fenômeno: a ausência de concordância temporal entre a cópula e o verbo da sentença encaixada.
Validation of the CrIS fast physical NH3 retrieval with ground-based FTIR
Authors: Dammers Enrico , Shephard Mark W. , Palm Mathias , Cady-Pereira Karen , Capps Shannon , Lutsch Erik , Strong Kim , Hannigan James W. , Ortega Ivan , Toon Geoffrey C. , Stremme Wolfgang , Grutter Michel , Jones Nicholas , Smale Dan , Siemons Jacob , Hrpcek Kevin , Tremblay Denis , Schaap Martijn , Notholt Justus , Erisman Jan Willem 
Published: July 2017

Abstract. Presented here is the validation of the CrIS (Cross-track Infrared Sounder) fast physical NH3 retrieval (CFPR) column and profile measurements using ground-based Fourier transform infrared (FTIR) observations. We use the total columns and profiles from seven FTIR sites in the Network for the Detection of Atmospheric Composition Change (NDACC) to validate the satellite data products. The overall FTIR and CrIS total columns have a positive correlation of r  =  0.77 (N  =  218) with very little bias (a slope of 1.02). Binning the comparisons by total column amounts, for concentrations larger than 1.0  ×  1016 molecules cm−2, i.e. ranging from moderate to polluted conditions, the relative difference is on average ∼ 0–5 % with a standard deviation of 25–50 %, which is comparable to the estimated retrieval uncertainties in both CrIS and the FTIR. For the smallest total column range (< 1.0  × 1016 molecules cm−2) where there are a large number of observations at or near the CrIS noise level (detection limit) the absolute differences between CrIS and the FTIR total columns show a slight positive column bias. The CrIS and FTIR profile comparison differences are mostly within the range of the single-level retrieved profile values from estimated retrieval uncertainties, showing average differences in the range of  ∼ 20 to 40 %. The CrIS retrievals typically show good vertical sensitivity down into the boundary layer which typically peaks at  ∼ 850 hPa (∼ 1.5 km). At this level the median absolute difference is 0.87 (std  =  ±0.08) ppb, corresponding to a median relative difference of 39 % (std  =  ±2 %). Most of the absolute and relative profile comparison differences are in the range of the estimated retrieval uncertainties. At the surface, where CrIS typically has lower sensitivity, it tends to overestimate in low-concentration conditions and underestimate in higher atmospheric concentration conditions.
On The Implicit Negation via Subjunctive Mood
Authors: Mnatsyan Azganush 
Published: July 2017

Extensive research on negation in linguistics has not only led to new theories but also dictated a range of ideas.In the frame of this article an effort is made to reveal semantic-functional traits of verb forms from a modal perspective. The basis of the study is mood-negation correlation. The formal description of the categories allows us to see the immediate differences of negation and changeability of form and meaning in different mood forms.The corpus at our data shows that the semantic value of implicit negative constructions is context-driven as a result of the close interaction of negation with modal categories. In the paradigm of the subjunctive form some affirmative constructions come into use as negatively charged linguistic items. Moreover, mood categories conflate under the rubric of realis - irrealis.
The perceptions of women on child birthing in a public-health facility in a peri-urban area in Kavango east region, Namibia
Authors: Muntenda Bartholomeus M , Nuuyoma Vistolina , Stern Ruth 
Published: July 2017

Purpose: The study explored the perceptions of women on child birthing in a public-health facility in a peri-urban area of the Kavango east region.Methods: A qualitative case study was conducted with women residing in Kehemu settlement, who have given birth either using a public-health facility or outside a public facility with the assistance of traditional birth attendants. Data were collected via three focus group discussions with 21 women (n = 21) who were purposively selected. Discussions were conducted in a local language and all were audio recorded with the participants’ permission. Data were transcribed and translated into English for analysis. The process of data reduction was used to analyse the study data.Results: Women were found to prefer child birthing at a public hospital because they considered nurses and midwives to be knowledgeable, caring and skilled people with regard to managing a woman in labour. However, although some women prefer to deliver at a public hospital, circumstances such as lack of transport – especially at night-time – prevent them from reaching the hospital. Moreover, some women indicated that nurses’ attitudes, the ill timing of labour and the wrong advice were hindrances to public hospital child birthing. Women stated more nurses, more supplies of the items used in child birthing, the provision of pain relief during labour, and a change in nurses’ attitudes as being some of the improvements which would make public hospital maternity wards more user-friendly.Conclusions: The women perceived child birthing at the public-health facility as being generally good, affordable and acceptable. However, there is a need for more interventions to make the public-health facility more user-friendly and accessible to all.
Application of Poisson Mixed Combined Models for Identifying Correlations of CD4 Count Progression in HIV Infected TB Patients During ART Treatment Period
Authors: Temesgen Aboma 
Published: July 2017

CD4 count is used to measures the number CD4 cells in the blood mostly during ART treatment to know the risk progression of HIV in the HIV infected patients. This continuously measured CD4 count during the treatment period results longitudinal data having correlation and over dispersion effects. While modeling such data to identify associated factors of change in CD4 count to monitor the progression of HIV most of the study did not considered these two main effects. The main aim of this study was also to consider these two main effect to identify the risk factors CD4 count progression based on 239 HIV infected TB patients who were 18 years old and above taking ART treatment from $1^{st}$ September 2009 to $1^{st}$ July 2014 at Jimma University Specialize Hospital. The result of study showed Poisson normal Gamma combine model which handles correlation and over dispersion effects of CD4 count simultaneously was an appropriate fit of the data among different Poisson mixed combined models considered for the study based on Akaki information criteria (AIC)comparisons. The estimated model depicts linear time and it's interaction effect with functional status category group of the patients have positive effect whereas quadratic time has the negative effect on the progression of CD4 count. The model also showed baseline bedridden and ambulatory functional status group patients has lower average CD4 count measurements in comparison with working functional status group patients counterparts. Therefore, while modeling CD4 count correlation and over dispersion should be taken in to consideration since the CD4 count value was correlated due to repeated measurement and it's variance larger than mean leading to over dispersion. Being at bedridden, ambulatory functional status at baseline in comparison with working functional status group and having quadratic time effects were also the associated risk factors that lowers the CD4 count measurements of the patients during the ART treatment period at the study area.
Het commissierapport: inhoud als uitdrukking van een proces
Authors: Schulz Martin , Ferket Jony , van der Steen Martijn 
Published: July 2017

The Commission Report: Content as the expression of an ongoing process
In this contribution we analyze the content of the Veerman Commission’s report that in 2007/2008 advised the Dutch government on the necessity of measures to protect the coast against future rising waters and other climatological and environmental changes and challenges. We conclude that the content of the report is in itself an expression of the ongoing social and governmental debate and process that tries to create a sense of urgency since there is no real immediate crisis to facilitate changes. Thus the report is not only the result of the work of the commission (though its firm statements on the necessity of measures were clearly heard), but at the same time the reflection of an ongoing debate which also creates a new challenge for stakeholders in the water domain. The organizational recommendations of the commission to place the protection against rising waters as far away from day to day politics as possible have all been put into action, which is a noteworthy result. Still, it is the ongoing process between stakeholders that will determine the actual measures to be taken by the government water related bodies.
‘Governing Governance’: zware kost in een Jip-en-Janneke-jasje
Authors: van der Heijden Jeroen 
Published: July 2017

Review of ‘Governing Governance’
The manifest Governing Governance: A Liberal-Democratic View on Governance by Relationships, Bureaucracies and Markets in the 21st Century aims to please a wide range of audiences. Yet in trying to do so it does not seem to please any. The manifest may be applauded for its clear and concise discussion of the complicated concept of governance. Yet, it falls short in being a truly engaging text as it is somewhat outdated, it lacks interaction with the wider world beyond the Netherlands (and Belgium), and it is too optimistic in terms of what may be achieved through collaborative or new governance and deliberative democracy. All this may very well explain why the manifest has, as of yet, not received a great deal of attention.
Consultations, Citizen Narratives and Evidence-Based Regulation
Authors: Ranchordás Sofia 
Published: July 2017

Consultations, Citizen Narratives and Evidence-Based Regulation: The Strange Case of the Consultation on the Collaborative Economy
The 2015 Better Regulation Communication advocates an evidence-based approach to regulation, which includes better consultations and broader civic engagement. In this article, I consider the recent EU public consultation on the regulatory environment of online platforms and the collaborative economy. I enquire in this context whether citizens were seriously regarded as evidence providers and how their knowledge that materialized in individual narratives could contribute to more legitimate and thus better regulation. I argue that an evidence-based approach to regulation should also include citizen narratives as they can provide first-hand and diverse perspectives, which might not be considered in standard consultation questions. I contend that citizen narratives can be particularly useful in complex and rapidly evolving fields where there is still little empirical evidence and where participants are likely to have diverse personal experiences. Drawing on the literature on narratives, I contend that this method of collecting information can help regulators identify new problems and structure solutions in rapidly changing and diverse regulatory fields such as the collaborative economy.