bluuebunny/crossref_metadata_2025_split huggingface dataset:
Managerial Ambivalence and Electronic Civic Engagement: The Role of Public Manager Beliefs and Perceived Needs
Authors: Zhang Fengxiu , Feeney Mary K. 
Published: September 2017

AbstractThis article addresses an important yet often neglected component of electronic civic engagement efforts: managerial ambivalence about public participation. We examine how managers’ beliefs about public participation and their perceived needs for participation in agency decision making work together to shape electronic engagement efforts. Based on observational data collected in 2010 and 2014 and data from a 2014 survey of managers in 500 U.S. municipal governments, we find that managerial beliefs about participation and their perceived needs for participation are two valid and separate constructs. There is a positive relationship between managerial beliefs and electronic engagement. Perceived needs for participation interact with managers’ beliefs to affect electronic civic engagement. A high level of perceived needs for participation reinforces the effect of managerial beliefs on electronic engagement efforts, but a low level does not offset the effect of managerial beliefs on electronic engagement.
Quality screening and trade intermediaries: Evidence from China
Authors: Poncet Sandra , Xu Meina 
Published: September 2017

AbstractWe examine the quality‐screening role played by intermediaries in international trade, exploiting export data at the product level for Chinese exporters. We uncover substantial heterogeneity among intermediaries, and distinguish two types: generalized and specialized intermediaries. We find strong evidence of a quality‐verification role for specialized intermediaries: they are more prevalent in products with greater quality dispersion among local exporters, and export goods of higher quality than do generalized intermediaries. Our results suggest that specialized intermediaries have the capacity to reduce the incidence of quality problems.
An enhanced approach for surface flow routing over drainage‐constrained triangulated irregular networks
Authors: Zhang Fangli , Zhou Qiming , Li Qingquan , Wu Guofeng , Liu Jun 
Published: September 2017

AbstractThe accuracy and efficiency of the simulations in distributed hydrological models must depend on the proper estimation of flow directions and paths. Numerous studies have been carried out to delineate the drainage patterns based on gridded digital elevation models (DEMs). The triangulated irregular network (TIN) has been increasingly applied in hydrological applications due to the advantages of high storage efficiency and multi‐scale adaptive performance. Much of the previous literature focuses mainly on filling the depressions on gridded DEMs rather than treating the special cases in TIN structures, which has hampered its applications to hydrological models. This study proposes a triangulation‐based solution for the removal of flat areas and pits to enhance the simulation of flow routing on triangulated facet networks. Based on the drainage‐constrained TIN generated from only a gridded DEM by the compound point extraction (CPE) method, the inconsistent situations including flat triangles, V‐shape flat edges and sink nodes are respectively identified and rectified. The optimization algorithm is an iterative process of TIN reconstruction, in which the flat areas are generalized into their center points and the pits are rectified by embedding break lines. To verify the proposed algorithm and investigate the potential for flow routing, flow paths of steepest descent are derived by the vector‐based tracking algorithm based on the optimized TIN. A case study of TIN optimization and flow path tracking was performed on a real‐world DEM. The outcomes indicate that the proposed approach can effectively solve the problem of inconsistencies without a significant loss in accuracy of the terrain model.
Inferring the home locations of Twitter users based on the spatiotemporal clustering of Twitter data
Authors: Lin Jie , Cromley Robert G. 
Published: September 2017

AbstractResidential locations play an important role in understanding the form and function of urban systems. However, it is impossible to release this detailed information publicly, due to the issue of privacy. The rapid development of location‐based services and the prevalence of global position system (GPS)‐equipped devices provide an unprecedented opportunity to infer residential locations from user‐generated geographic information. This article compares different approaches for predicting Twitter users' home locations at a precise point level based on temporal and spatial features extracted from geo‐tagged tweets. Among the three deterministic approaches, the one that estimates the home location for each user by finding the weighted most frequently visited (WMFV) cluster of that user always provides the best performance when compared with the other two methods. The results of a fourth approach, based on the support vector machine (SVM), are severely affected by the threshold value for a cluster to be identified as the home.
Genome and Plasmid Sequences of Escherichia coli KV7, an Extended-Spectrum β-Lactamase Isolate Derived from Feces of a Healthy Pig
Authors: Bateman Michael D. , de Vries Stefan P. W. , Gupta Srishti , Guardabassi Luca , Cavaco Lina M. , Grant Andrew J. , Holmes Mark A. 
Published: September 2017

ABSTRACT

We present single-contig assemblies for
Escherichia coli
strain KV7 (serotype O27, phylogenetic group D) and its six plasmids, isolated from a healthy pig, as determined by PacBio RS II and Illumina MiSeq sequencing. The chromosome of 4,997,475 bp and G+C content of 50.75% harbored 4,540 protein-encoding genes.
Small Circular Rep-Encoding Single-Stranded DNA Genomes in Peruvian Diarrhea Virome
Authors: Altan Eda , Del Valle Mendoza Juana , Deng Xutao , Phan Tung G. , Sadeghi Mohammadreza , Delwart Eric L. 
Published: September 2017

ABSTRACT
Metagenomic analysis of diarrhea samples revealed the presence of numerous human enteric viruses and small circular Rep-encoding single-stranded DNA (CRESS-DNA) genomes. One such genome was related to smacoviruses, while eight others were related to genomes reported in the feces of different mammals. The tropism of these CRESS-DNA viruses remains unknown.
Complete Genome Sequence of Ureibacillus thermosphaericus A1, a Thermophilic Bacillus Isolated from Compost
Authors: Akita Hironaga , Kimura Zen-ichiro , Matsushika Akinori 
Published: September 2017

ABSTRACT

Ureibacillus thermosphaericus
A1 was isolated from compost collected in Munakata City, Fukuoka Prefecture, Japan. Here, we report the first complete genome sequence of
U. thermosphaericus
. The complete genome of this strain consists of 3,488,104 bp with a GC content of 36.3% and comprises 3,362 predicted coding sequences.
Draft Genome Sequence of Pantoea agglomerans JM1, a Strain Isolated from Soil Polluted by Industrial Production of Beta-Lactam Antibiotics That Exhibits Valacyclovir-Like Hydrolase Activity
Authors: Zahradník Jiří , Plačková Martina , Palyzová Andrea , Marešová Helena , Kyslíková Eva , Kyslík Pavel 
Published: September 2017

ABSTRACT

Strain
Pantoea agglomerans
JM1 was isolated from the soil of a microbiome that had been exposed to polluting pharmaceuticals. The bacterium exhibited enzymatic activities useful for the biotransformation of beta-lactams. The genome of the strain was assembled and described, and the gene encoding valacyclovir-like hydrolase was identified.
Draft Genome Sequence of
Aspergillus persii
NIBRFGC000004109, Which Has Antibacterial Activity against Plant-Pathogenic Bacteria
Authors: Kim Jung A. , Jeon Jongbum , Park Sook-Young , Kim Ki-Tae , Choi Gobong , Nguyen Hoa Thi , Jeon Sun Jeong , Lee Hyang Burm , Bae Chang-Hwan , Yang Hee-sun , Yeo Joo-Hong , Kim Jin-Cheol , Lee Yong-Hwan , Kim Soonok 
Published: September 2017

ABSTRACT

The fungus
Aspergillus persii
strain NIBRFGC000004109 is capable of producing penicillic acid and showed antibacterial activity against various plant-pathogenic bacteria, including
Xanthomonas arboricola
pv. pruni. Here, we report the first draft whole-genome sequence of
A. persii
. The assembly comprises 38,414,373 bp, with 12 scaffolds.
Genome Sequence of an H9N2 Avian Influenza Virus Strain with Hemagglutinin-Neuraminidase Combination, Isolated from a Quail in Guangxi, Southern China
Authors: Xie Liji , Xie Zhixun , Li Dan , Luo Sisi , Zhang Minxiu , Huang Li , Xie Zhiqin , Huang Jiaoling , Zhang Yanfang , Zeng Tingting , Deng Xianwen 
Published: September 2017

ABSTRACT
We isolated a strain of H9N2 avian influenza virus from a quail in southern China in May 2015 and named it A/quail/Guangxi/198Q39/2015. All eight gene segments of the strain were sequenced. Sequence analysis indicated that the amino acid motif of the hemagglutinin cleavage site of this H9N2 virus was RSSR↓GLF, which is a typical characteristic of the low pathogenic avian influenza virus. This study will help in better understanding the epidemiology and molecular characteristics of avian influenza virus in wild birds.
Draft Genome Sequence of Zoonotic Streptococcus canis Isolate G361
Authors: Eichhorn Inga , van der Linden Mark , Jarek Michael , Fulde Marcus 
Published: September 2017

ABSTRACT

Here, we report the draft genome sequence of an SCM-positive
Streptococcus canis
strain, G361, isolated from a vaginal swab of a 40-year-old woman. The draft genome comprises 2,045,931 bp in 62 contigs.
Insights into the Genome of the Anaerobic Acetogen Sporomusa silvacetica DSM 10669
Authors: Humphreys Jonathan R. , Daniel Rolf , Poehlein Anja 
Published: September 2017

ABSTRACT

Sporomusa silvacetica
is a spore-forming, anaerobic acetogen isolated from soil derived from east central Germany. The genome contains genes of the Wood-Ljungdahl pathway required for carbon fixation and genes involved in the biosynthesis of the amino acid pyrrolysine. The genome (5.92 Mb) harbors 4,355 predicted protein-encoding genes.
Draft Genome Sequences of Three Coxiella burnetii Strains Isolated from Q Fever Patients
Authors: Beare Paul A. , Jeffrey Brendan M. , Martens Craig A. , Heinzen Robert A. 
Published: September 2017

ABSTRACT

In the current study, we determined the draft genome sequences of three
Coxiella burnetii
human disease isolates. The
Coxiella burnetii
Turkey (RSA315) and Dyer (RSA345) strains were isolated from acute Q fever patients, while the Ko (Q229) strain was isolated from a Q fever endocarditis patient.
Complete Genome Sequence of a Novel Bioflocculant-Producing Strain, Microbacterium paludicola CC3
Authors: Liu Weijie , Liu Cong , Sun Di 
Published: September 2017

ABSTRACT

Microbacterium paludicola
CC3 exhibits the capability to produce polysaccharide bioflocculants. Here, we report the whole-genome sequence of
M. paludicola
CC3, which may be helpful in understanding the genetic basis of the biosynthesis of polysaccharide bioflocculants as well as in promoting its production and application in industrial fields.
Genome Sequence of Bacillus safensis Strain Ingolstadt Isolated from the Pectoralis Pouch of a Patient with Defibrillator-Related Surgery
Authors: Dematheis Flavia , Antwerpen Markus H. , Grass Gregor , Walter Mathias C. , Borgmann Stefan 
Published: September 2017

ABSTRACT

We report the draft genome sequence of clindamycin-resistant
Bacillus safensis
strain Ingolstadt isolated from a patient with bacterial colonization after heart surgery. The draft genome comprises 3.75 Mbp and harbors 3,793 predicted protein-encoding genes and a small plasmid.
Control of
Clostridium difficile
Infection by Defined Microbial Communities
Authors: Collins James , Auchtung Jennifer M. 
Published: September 2017

ABSTRACT

Each year in the United States, billions of dollars are spent combating almost half a million
Clostridium difficile
infections (CDIs) and trying to reduce the ∼29,000 patient deaths in which
C. difficile
has an attributed role. In Europe, disease prevalence varies by country and level of surveillance, though yearly costs are estimated at €3 billion. One factor contributing to the significant health care burden of
C. difficile
is the relatively high frequency of recurrent CDIs. Recurrent CDI, i.e., a second episode of symptomatic CDI occurring within 8 weeks of successful initial CDI treatment, occurs in ∼25% of patients, with 35 to 65% of these patients experiencing multiple episodes of recurrent disease. Using microbial communities to treat recurrent CDI, either as whole fecal transplants or as defined consortia of bacterial isolates, has shown great success (in the case of fecal transplants) or potential promise (in the case of defined consortia of isolates). This review will briefly summarize the epidemiology and physiology of
C. difficile
infection, describe our current understanding of how fecal microbiota transplants treat recurrent CDI, and outline potential ways that knowledge can be used to rationally design and test alternative microbe-based therapeutics.
Sources of Fungal Genetic Variation and Associating It with Phenotypic Diversity
Authors: Taylor John W. , Branco Sara , Gao Cheng , Hann-Soden Chris , Montoya Liliam , Sylvain Iman , Gladieux Pierre 
Published: September 2017

ABSTRACT

The first eukaryotic genome to be sequenced was fungal, and there continue to be more sequenced genomes in the kingdom Fungi than in any other eukaryotic kingdom. Comparison of these genomes reveals many sources of genetic variation, from single nucleotide polymorphisms to horizontal gene transfer and on to changes in the arrangement and number of chromosomes, not to mention endofungal bacteria and viruses. Population genomics shows that all sources generate variation all the time and implicate natural selection as the force maintaining genome stability. Variation in wild populations is a rich resource for associating genetic variation with phenotypic variation, whether through quantitative trait locus mapping, genome-wide association studies, or reverse ecology. Subjects of studies associating genetic and phenotypic variation include model fungi, e.g.,
Saccharomyces
and
Neurospora
, but pioneering studies have also been made with fungi pathogenic to plants, e.g.,
Pyricularia
(=
Magnaporthe
),
Zymoseptoria
, and
Fusarium
, and to humans, e.g.,
Coccidioides
,
Cryptococcus
, and
Candida
.
Foot orthoses for plantar heel pain: a systematic review and meta-analysis
Authors: Whittaker Glen A , Munteanu Shannon E , Menz Hylton B , Tan Jade M , Rabusin Chantel L , Landorf Karl B 
Published: September 2017

ObjectiveTo investigate the effectiveness of foot orthoses for pain and function in adults with plantar heel pain.DesignSystematic review and meta-analysis. The primary outcome was pain or function categorised by duration of follow-up as short (0 to 6 weeks), medium (7 to 12 weeks) or longer term (13 to 52 weeks).Data sourcesMedline, CINAHL, SPORTDiscus, Embase and the Cochrane Library from inception to June 2017.Eligibility criteria for selecting studiesStudies must have used a randomised parallel-group design and evaluated foot orthoses for plantar heel pain. At least one outcome measure for pain or function must have been reported.ResultsA total of 19 trials (1660 participants) were included. In theshort term, there was very low-quality evidence that foot orthoses do not reduce pain or improve function. In themedium term, there was moderate-quality evidence that foot orthoses were more effective than sham foot orthoses at reducing pain (standardised mean difference −0.27 (−0.48 to −0.06)). There was no improvement in function in the medium term. In thelonger term, there was very low-quality evidence that foot orthoses do not reduce pain or improve function. A comparison of customised and prefabricated foot orthoses showed no difference at any time point.ConclusionThere is moderate-quality evidence that foot orthoses are effective at reducing pain in the medium term, however it is uncertain whether this is a clinically important change.
Serpinb3 is Overexpressed in the Liver in Presence of Iron Overload
Authors: Turato Cristian , Kent Patricia , Sebastiani Giada , Cannito Stefania , Morello Elisabetta , Terrin Liliana , Biasiolo Alessandra , Simonato Davide , Parola Maurizio , Pantopoulos Kostas , Pontisso Patrizia 
Published: September 2017

Iron overload results in cellular toxicity, tissue injury, organ fibrosis and increased risk of neoplastic transformation. SerpinB3 is a serine protease inhibitor overexpressed in the liver in oxidative stress conditions, able to induce fibrosis and increased risk of malignant transformation. Aim of the present study was to assess the effect of iron overload on SerpinB3 expression in the liver using in vivo and in vitro models. The expression of Serpinb3 was assessed in the liver of hemojuvelin knockout mice (Hjv–/–), an established model of hereditary hemochromatosis, and of wild type control mice, following dietary or pharmacological iron manipulation. To assess the direct effect of iron in vitro, cell lines were treated with different concentration of hemin or with an iron chelator. Hepatic Serpinb3 mRNA and protein were highly expressed in Hjv–/– mice, but not in wild type controls fed with a standard diet. Serpinb3 became detectable in wild type mice fed with a high iron diet or injected with iron dextran; these treatments further induced Serpinb3 expression in Hjv–/– mice. Livers expressing Serpinb3 showed a positive staining also for HIF-2α in the same areas. Hemin promoted induction of SerpinB3 mRNA in HeLa and HA22T/VGH cells, but a mild stimulation of SerpinB3 promoter activity in HeLa and Huh7 cells. In conclusion, Serpinb3 is strongly induced by iron in the mouse liver. The molecular link between iron, ROS and SerpinB3 seems to be HIF-2α, which is induced by iron overload and was previously found capable of up-regulating SerpinB3 at the transcriptional level.
Anti-inflammatory approaches to ischaemic stroke prevention
Authors: Kelly Peter J , Murphy Sean , Coveney Sarah , Purroy Francisco , Lemmens Robin , Tsivgoulis Georgios , Price Chris 
Published: September 2017

Stroke is a major cause of neurological morbidity and mortality. Atherosclerosis is a major contributor to first and recurrent stroke. A growing evidence base indicates that inflammation is a key process in the pathogenesis of atherosclerosis, leading to thromboembolic events. In this review, we summarise the evidence linking inflammation to stroke risk and discuss clinical trials addressing the ’inflammation hypothesis' in coronary disease and stroke.Trial registration numberCONVINCE trial ClinicalTrials.gov number; NCT 02898610; Pre-results.
Polysomnographic phenotypes and their cardiovascular implications in obstructive sleep apnoea
Authors: Zinchuk Andrey V , Jeon Sangchoon , Koo Brian B , Yan Xiting , Bravata Dawn M , Qin Li , Selim Bernardo J , Strohl Kingman P , Redeker Nancy S , Concato John , Yaggi Henry K 
Published: September 2017

BackgroundObstructive sleep apnoea (OSA) is a heterogeneous disorder, and improved understanding of physiologic phenotypes and their clinical implications is needed. We aimed to determine whether routine polysomnographic data can be used to identify OSA phenotypes (clusters) and to assess the associations between the phenotypes and cardiovascular outcomes.MethodsCross-sectional and longitudinal analyses of a multisite, observational US Veteran (n=1247) cohort were performed. Principal components-based clustering was used to identify polysomnographic features in OSA’s four pathophysiological domains (sleep architecture disturbance, autonomic dysregulation, breathing disturbance and hypoxia). Using these features, OSA phenotypes were identified by cluster analysis (K-means). Cox survival analysis was used to evaluate longitudinal relationships between clusters and the combined outcome of incident transient ischaemic attack, stroke, acute coronary syndrome or death.ResultsSeven patient clusters were identified based on distinguishing polysomnographic features: ‘mild’, ‘periodic limb movements of sleep (PLMS)’, ‘NREM and arousal’, ‘REM and hypoxia’, ‘hypopnoea and hypoxia’, ‘arousal and poor sleep’ and ‘combined severe’. In adjusted analyses, the risk (compared with ‘mild’) of the combined outcome (HR (95% CI)) was significantly increased for ‘PLMS’, (2.02 (1.32 to 3.08)), ‘hypopnoea and hypoxia’ (1.74 (1.02 to 2.99)) and ‘combined severe’ (1.69 (1.09 to 2.62)). Conventional apnoea–hypopnoea index (AHI) severity categories of moderate (15≤AHI<30) and severe (AHI ≥30), compared with mild/none category (AHI <15), were not associated with increased risk.ConclusionsAmong patients referred for OSA evaluation, routine polysomnographic data can identify physiological phenotypes that capture risk of adverse cardiovascular outcomes otherwise missed by conventional OSA severity classification.
Role of eosinophils in the initiation and progression of pancreatitis pathogenesis
Authors: Manohar Murli , Verma Alok K. , Venkateshaiah Sathisha Upparahalli , Mishra Anil 
Published: September 2017

Eosinophilic pancreatitis (EP) is reported in humans; however, the etiology and role of eosinophils in EP pathogenesis are poorly understood and not well explored. Therefore, it is interesting to examine the role of eosinophils in the initiation and progression of pancreatitis pathogenesis. Accordingly, we performed anti-major basic protein immunostaining, chloroacetate esterase, and Masson’s trichrome analyses to detect eosinophils, mast cells, and collagen in the tissue sections of mouse and human pancreas. Induced eosinophils accumulation and degranulation were observed in the tissue sections of human pancreatitis, compared with no eosinophils in the normal pancreatic tissue sections. Similarly, we observed induced tissue eosinophilia along with mast cells and acinar cells atrophy in cerulein-induced mouse model of chronic pancreatitis. Additionally, qPCR and ELISA analyses detected induced transcript and protein levels of proinflammatory and profibrotic cytokines, chemokines like IL 5, IL-18, eotaxin-1, eotaxin-2, TGF-β1, collagen-1, collagen-3, fibronectin, and α-SMA in experimental pancreatitis. Mechanistically, we show that eosinophil-deficient GATA1 and endogenous IL-5-deficient mice were protected from the induction of proinflammatory and profibrotic cytokines, chemokines, tissue eosinophilia, and mast cells in a cerulein-induced murine model of pancreatitis. These human and experimental data indicate that eosinophil accumulation and degranulation may have a critical role in promoting pancreatitis pathogenesis including fibrosis. Taken together, eosinophil tissue accumulation needs appropriate attention to understand and restrict the progression of pancreatitis pathogenesis in humans.NEW & NOTEWORTHY The present study for the first time shows that eosinophils accumulate in the pancreas and promote disease pathogenesis, including fibrosis in earlier reported cerulein-induced experimental models of pancreatitis. Importantly, we show that GATA-1 and IL-5 deficiency protects mice form the induction of eosinophil active chemokines, and profibrotic cytokines, including accumulation of tissue collagen in an experimental model of pancreatitis. Additionally, we state that cerulein-induced chronic pancreatitis is independent of blood eosinophilia.