bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Significance of SCC and CEA mRNA in the Pleural Cavity After Lymphadenectomy in Esophageal Cancer Patients who Underwent Preoperative Treatment
Authors: Sugimura Keijiro , Miyata Hiroshi , Motoori Masaaki , Omori Takeshi , Fujiwara Yoshiyuki , Yano Masahiko 
Published: September 2017

AbstractBackgroundThis report focuses on the surgical manipulation and spread of cancer cells. Our previous study suggested an association between a poor prognosis and positive pleural lavage cytology after resection of esophageal cancer without preoperative treatment. However, little is known regarding the clinical significance of the lavage procedure in esophageal cancer patients who undergo preoperative treatment.MethodsA cohort of 94 patients with squamous cell carcinoma of the esophagus who underwent esophagectomy with radical lymph node dissection was prospectively analyzed for free cancer cells in the pleural cavity after mediastinal lymphadenectomy. Reverse transcription–polymerase chain reaction was performed to detect free cancer cells in the pleural lavage fluid by measuring squamous cell carcinoma‐related antigen (SCC) and carcinoembryonic antigen (CEA).ResultsForty‐two patients (44.7%) were positive for SCC after thoracic lymphadenectomy, and 15 patients (15.9%) were positive for CEA. SCC positivity was significantly associated with venous invasion (p = 0.037) and with the clinical response to preoperative treatment (p = 0.001). Furthermore, SCC positivity was associated with poor prognosis compared with negative SCC (p = 0.026). Multivariate analysis revealed that SCC positivity was an independent prognostic factor. Regarding recurrence patterns, SCC positivity tended to be associated with hematogenous recurrence (p = 0.063). Conversely, positive CEA was not associated with any clinicopathological finding, treatment response, prognosis, or recurrence pattern.ConclusionsTumor spillage during the evaluated surgical manipulation was assessed in esophageal cancer patients who underwent preoperative treatment. Tumor spillage as evaluated by SCC mRNA was associated with a poor prognosis.
State of the art und Zukunft der Vitrektomie – Techniken und Instrumente
Authors: Mennel Stefan , Bechrakis Nikolaos E. , Binder Susanne , Haas Anton 
Published: September 2017

Zusammenfassung
Hintergrund
Durch die Einführung der Pars plana Vitrektomie ist eine Vielzahl von vitreoretinalen Erkrankungen behandelbar geworden. Kontinuierliche Verbesserungen der Geräte, Instrumente und zusätzliche Hilfsmittel sowie neue Techniken kennzeichnen den enormen Fortschritt der vitreoretinalen Chirurgie.

Methoden
In einer Literaturübersicht werden die Operationstechniken verschiedenster vitreoretinaler Eingriffe analysiert. Sofern aufgrund aktueller Entwicklungen verschiedene Optionen in der Operationstechnik bestehen, werden Experten auf dem Gebiet der vitreoretinalen Chirurgie über ihre Erfahrung und ihre Empfehlung befragt.

Ergebnisse
Die 20 Gauge Pars plana Vitrektomie wurde größtenteils durch die Verwendung von Trokaren (23, 25 und 27 Gauge) mit entsprechenden Instrumenten mit kleinerem Durchmesser abgelöst. Die Pars plana Vitrektomie ist nahtlos möglich, trotzdem kann es notwendig sein, eine zusätzliche Sicherung der Wunde mit einer Naht durchzuführen, um Leckage und Hypotonie zu vermeiden. Die Visualisierung des Glaskörpers erfolgt zunehmend mit Triamcinolon, epiretinale Gliosen und die Membrana limitans interna werden routinemäßig mit Vitalfarbstoffen dargestellt. Bei der Operationstechnik beim Makulaforamen und bei der Ablatio retinae zeigen sich sowohl bei aktuellen Publikationen als auch unter den Experten Variationen.

Schlussfolgerungen
Die Pars plana Vitrektomie hat sich aufgrund der Weiterentwicklung speziell im Bereich der Trokar Systeme, Vitrektome, der Weitwinkel-Beobachtungssysteme sowie dank neuer Techniken zu einer essentiellen OP-Methode für ein weites Indikationsspektrum entwickelt. Dies ist die Basis der Behandlung verschiedenster vitreoretinaler Erkrankungen mit immer weniger Operationstrauma, weniger intraoperativen und postoperativen Komplikationen und gutem Therapieerfolg. Unterschiedliche Operationsvarianten, neue Techniken und die Weiterentwicklung der Geräte und Instrumente sind die Basis für einen auch zukünftigen Fortschritt der Pars plana Vitrektomie.
Understanding positive immigrant youth adaptation in the context of multiculturalism☆
Authors: Sam David L. 
Published: September 2017

ABSTRACTThis commentary highlights some of the strengths of the papers in this special issue focusing on how they advance acculturation research; how they link immigrant youth research with positive youth development and how they provide some insights into understanding how immigrant youth thrive in their adoptive societies. The commentary takes as its point of departure the prototypical research question for immigrant youth research within the perspective of positive youth development and the ultimate goals of acculturation research, i.e., to (i) promote positive psychological acculturation and the well‐being of individuals and (ii) the attainment of harmonious intercultural relations among all groups in contact, and argues that the four papers fall short in addressing the second goal. The second goal is subsequently linked to multiculturalism and suggest that to do full justice to the prototypical research question, more attention should be directed to incorporating indicators of multiculturalism in acculturation research.
Lessons Learned From Implementing an Incident Command System During a Local Multiagency Response to a Legionnaires’ Disease Cluster in Sydney, NSW
Authors: Quinn Emma , Johnstone Travers , Najjar Zeina , Cains Toni , Tan Geoff , Huhtinen Essi , Nilsson Sven , Burgess Stuart , Dunn Matthew , Gupta Leena 
Published: September 2017

AbstractThe incident command system (ICS) provides a common structure to control and coordinate an emergency response, regardless of scale or predicted impact. The lessons learned from the application of an ICS for large infectious disease outbreaks are documented. However, there is scant evidence on the application of an ICS to manage a local multiagency response to a disease cluster with environmental health risks. The Sydney Local Health District Public Health Unit (PHU) in New South Wales, Australia, was notified of 5 cases of Legionnaires’ disease during 2 weeks in May 2016. This unusual incident triggered a multiagency investigation involving an ICS with staff from the PHU, 3 local councils, and the state health department to help prevent any further public health risk. The early and judicious use of ICS enabled a timely and effective response by supporting clear communication lines between the incident controller and field staff. The field team was key in preventing any ongoing public health risk through inspection, sampling, testing, and management of water systems identified to be at-risk for transmission of legionella. Good working relationships between partner agencies and trust in the technical proficiency of environmental health staff aided in the effective management of the response. (Disaster Med Public Health Preparedness. 2018;12:539–542)
Der Titelrekognitionstest für das Vorschulalter (TRT-VS)
Authors: Grolig Lorenz , Cohrdes Caroline , Schroeder Sascha 
Published: September 2017

Zusammenfassung. Studien haben gezeigt, dass bereits im Vorschulalter ein positiver Zusammenhang zwischen Lesevolumen und Sprachentwicklung besteht. Eine objektive und ökonomische Methode zur Erfassung des Lesevolumens sind Titelrekognitionstests. Hierbei geben die Teilnehmenden für eine Reihe von Buchtiteln an, ob ihnen diese bekannt sind. Um Ratetendenzen zu minimieren, enthält der Test auch fiktive Buchtitel. Wir beschreiben die Entwicklung des Titelrekognitionstests für das Vorschulalter (TRT-VS) und stellen anhand von 2 Validierungsstudien seine psychometrischen Eigenschaften vor. In Studie 1 untersuchen wir seine Reliabilität sowie Personen- und Itemkennwerte in einer Stichprobe von Kindern und jungen Erwachsenen. Studie 2 analysiert den Zusammenhang zwischen TRT-VS, häuslicher Lernumgebung und Vorläuferfertigkeiten des Lesens in einer Stichprobe von Vorschulkindern und deren Eltern. Die Studien belegen, dass der TRT-VS ein reliables Instrument zur Messung früher Leseerfahrungen ist und eine hohe Konstruktvalidität aufweist.
Entwicklung und Validierung einer Kurzversion des Fragebogeninventars für Freizeitinteressen (FIFI-K)
Authors: Nikstat Amelie , Höft Angelina , Lehnhardt Jessica , Hofmann Stephanie , Kandler Christian 
Published: September 2017

Zusammenfassung. Mit dem Fragebogeninventar für Freizeitinteressen (FIFI; Piepenburg & Kandler, 2016 ) steht nun erstmals ein deutschsprachiges Messinstrument für das Erwachsenenalter zur Verfügung, welches Freizeittätigkeiten hinsichtlich ihrer intrinsischen Wertigkeit (Freizeitinteresse) und Häufigkeit ihrer Ausübung (Freizeitaktivität) erfasst. Ziel der vorliegenden Arbeit war die Entwicklung einer Kurzversion (FIFI-K), um die Ökonomie der Erfassung bei gleichbleibender Heterogenität der Freizeitbereiche zu erhöhen. Das FIFI-K umfasst 67 Items, an denen die Struktur von Freizeitinteressenbereichen (21 Primärfaktoren und 5 Sekundärfaktoren) des FIFI bei 840 Probandinnen und Probanden mittels konfirmatorischer Faktorenanalysen bestätigt werden konnte. Basierend auf einer Substichprobe von 443 Probandinnen und Probanden fanden sich gute Retest-Reliabilitäten für entsprechende Freizeitbereiche. Zudem konnten konvergente Validität zwischen Freizeitinteresse und Freizeitaktivität und diskriminante Validität zwischen den Freizeitbereichen sowie prädiktive Validität von Freizeitinteressen und -aktivitäten zur Vorhersage psychischen Wohlbefindens bescheinigt werden. Das FIFI-K kann demnach als ökonomisches, reliables und valides Messinstrument für die individuelle Erfassung von Freizeitinteressen und -aktivitäten in Forschung und Praxis angewandt werden.
Das Eltern-Belastungs-Inventar (EBI)
Authors: Irlbauer-Müller Viktoria , Eichler Anna , A. Donhauser Johanna , E. Poehlmann Nina , Stemmler Mark , H. Moll Gunther , Kratz Oliver 
Published: September 2017

Zusammenfassung. Eltern-Kind-Interaktionen erfolgen natürlicherweise bidirektional. In der Folge sollte kinder- und jugendpsychiatrische / -psychotherapeutische (KJP–) Behandlung individuums- und kontextzentrierte Interventionen berücksichtigen. Dafür wurde eine dimensionale Erhebung elterlicher Belastung, wie sie mittels des Eltern-Belastungs-Inventars (EBI; Tröster, 2011 ) möglich ist, untersucht. Für N = 166 zu einem KJP-Erstkontakt vorstellige Kinder und Jugendliche (11 – 18 Jahre) wurden KJP-relevante Merkmale (inkl. kritischer Lebensereignisse), die elterliche Belastung (EBI) und drei Messungen der kindseitigen Symptomatik betrachtet (1. Elternurteil, 2. Selbsturteil, 3. klinisches Urteil). Zusätzlich wurde die Elternsicht auf die KJP-Behandlung als Be- oder Entlastung analysiert. Die Ergebnisse zeigen eine auffällig hohe elterliche Belastung. Diese Belastung korrelierte signifikant mit kritischen Lebensereignissen sowie mit dem Elternurteil. Verglichen mit dem Selbst- und dem klinischen Urteil erwies sich nur das Elternurteil als hoch signifikanter Prädiktor elterlicher Belastung, die für verschiedene KJP-Merkmale variierte. Die Inanspruchnahme der KJP-Behandlung wurde seitens der Eltern zumeist als Entlastung erlebt. Das EBI erlaubt folglich die Beschreibung eltern- bzw. familienbezogener Belastungen aus dimensionaler Perspektive, die eine fundierte kontextzentrierte Behandlungsplanung unterstützt.
Choose Your Words Wisely
Authors: Kotzur Patrick F. , Forsbach Nora , Wagner Ulrich 
Published: September 2017

Abstract. Differences in word connotations can have far-reaching consequences. We investigated the content, and emotional and behavioral consequences of the social perception of fled people as a function of their label (“refugees” vs. “asylum seekers”; “war refugees” vs. “economic refugees” vs. “refugees”) using a factorial survey (n = 389). Based on qualitative data on perceived intentions associated with the labels, we deducted predictions regarding differences in the Stereotype Content Model and Behavior from Intergroup Affect and Stereotypes Map. Participants evaluated refugees and asylum seekers similarly. Economic refugees were evaluated more negatively than war refugees or refugees, while the profiles of war refugees and refugees matched. These findings suggest that the choice of words to refer to fled people has profound consequences.
Loss of the vitamin D receptor in human breast and prostate cancers strongly induces cell apoptosis through downregulation of Wnt/β-catenin signaling
Authors: Zheng Yu , Trivedi Trupti , Lin Ruby CY , Fong-Yee Colette , Nolte Rick , Manibo Jeline , Chen Yunzhao , Hossain Musharraf , Horas Konstantin , Dunstan Colin , Zhou Hong , Seibel Markus J 
Published: September 2017

AbstractVitamin D co-regulates cell proliferation, differentiation and apoptosis in numerous tissues, including cancers. The known anti-proliferative and pro-apoptotic actions of the active metabolite of vitamin D, 1,25-dihydroxy-vitamin D [1,25(OH)2D] are mediated through binding to the vitamin D receptor (VDR). Here, we report on the unexpected finding that stable knockdown of VDR expression in the human breast and prostate cancer cell lines, MDA-MB-231 and PC3, strongly induces cell apoptosis and inhibits cell proliferation in vitro. Implantation of these VDR knockdown cells into the mammary fat pad (MDA-MB-231), subcutaneously (PC3) or intra-tibially (both cell lines) in immune-incompetent nude mice resulted in reduced tumor growth associated with increased apoptosis and reduced cell proliferation compared with controls. These growth-retarding effects of VDR knockdown occur in the presence and absence of vitamin D and are independent of whether cells were grown in bone or soft tissues. Transcriptome analysis of VDR knockdown and non-target control cell lines demonstrated that loss of the VDR was associated with significant attenuation in the Wnt/β-catenin signaling pathway. In particular, cytoplasmic and nuclear β-catenin protein levels were reduced with a corresponding downregulation of downstream genes such as Axin2, Cyclin D1, interleukin-6 (IL-6), and IL-8. Stabilization of β-catenin using the GSK-3β inhibitor BIO partly reversed the growth-retarding effects of VDR knockdown. Our results indicate that the unliganded VDR possesses hitherto unknown functions to promote breast and prostate cancer growth, which appear to be operational not only within but also outside the bone environment. These novel functions contrast with the known anti-proliferative nuclear actions of the liganded VDR and may represent targets for new diagnostic and therapeutic approaches in breast and prostate cancer.
Electrically conductive pili from pilin genes of phylogenetically diverse microorganisms
Authors: Walker David J F , Adhikari Ramesh Y , Holmes Dawn E , Ward Joy E , Woodard Trevor L , Nevin Kelly P , Lovley Derek R 
Published: September 2017

Abstract
The possibility that bacteria other than Geobacter species might contain genes for electrically conductive pili (e-pili) was investigated by heterologously expressing pilin genes of interest in Geobacter sulfurreducens. Strains of G. sulfurreducens producing high current densities, which are only possible with e-pili, were obtained with pilin genes from Flexistipes sinusarabici, Calditerrivibrio nitroreducens and Desulfurivibrio alkaliphilus. The conductance of pili from these strains was comparable to native G. sulfurreducens e-pili. The e-pili derived from C. nitroreducens, and D. alkaliphilus pilin genes are the first examples of relatively long (&gt;100 amino acids) pilin monomers assembling into e-pili. The pilin gene from Candidatus Desulfofervidus auxilii did not yield e-pili, suggesting that the hypothesis that this sulfate reducer wires itself with e-pili to methane-oxidizing archaea to enable anaerobic methane oxidation should be reevaluated. A high density of aromatic amino acids and a lack of substantial aromatic-free gaps along the length of long pilins may be important characteristics leading to e-pili. This study demonstrates a simple method to screen pilin genes from difficult-to-culture microorganisms for their potential to yield e-pili; reveals new sources for biologically based electronic materials; and suggests that a wide phylogenetic diversity of microorganisms may use e-pili for extracellular electron exchange.
Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration
Authors: Ichijo Ryo , Kobayashi Hiroki , Yoneda Saori , Iizuka Yui , Kubo Hirokazu , Matsumura Shigeru , Kitano Satsuki , Miyachi Hitoshi , Honda Tetsuya , Toyoshima Fumiko 
Published: September 2017

AbstractThe skin surface area varies flexibly in response to body shape changes. Skin homeostasis is maintained by stem cells residing in the basal layer of the interfollicular epidermis. However, how the interfollicular epidermal stem cells response to physiological body shape changes remains elusive. Here, we identify a highly proliferative interfollicular epidermal basal cell population in the rapidly expanding abdominal skin of pregnant mice. These cells express Tbx3 that is necessary for their propagation to drive skin expansion. The Tbx3+ basal cells are generated from Axin2+ interfollicular epidermal stem cells through planar-oriented asymmetric or symmetric cell divisions, and express transit-amplifying cell marker CD71. This biased division of Axin2+ interfollicular epidermal stem cells is induced by Sfrp1 and Igfbp2 proteins secreted from dermal cells. The Tbx3+ basal cells promote wound repair, which is enhanced by Sfrp1 and Igfbp2. This study elucidates the interfollicular epidermal stem cell/progeny organisation during pregnancy and suggests its application in regenerative medicine.
3D calcite heterostructures for dynamic and deformable mineralized matrices
Authors: Yi Jaeseok , Wang Yucai , Jiang Yuanwen , Jung Il Woong , Liu Wenjun , De Andrade Vincent , Xu Ruqing , Parameswaran Ramya , Peters Ivo R. , Divan Ralu , Xiao Xianghui , Sun Tao , Lee Youjin , Park Won Il , Tian Bozhi 
Published: September 2017

AbstractScales are rooted in soft tissues, and are regenerated by specialized cells. The realization of dynamic synthetic analogues with inorganic materials has been a significant challenge, because the abiological regeneration sites that could yield deterministic growth behavior are hard to form. Here we overcome this fundamental hurdle by constructing a mutable and deformable array of three-dimensional calcite heterostructures that are partially locked in silicone. Individual calcite crystals exhibit asymmetrical dumbbell shapes and are prepared by a parallel tectonic approach under ambient conditions. The silicone matrix immobilizes the epitaxial nucleation sites through self-templated cavities, which enables symmetry breaking in reaction dynamics and scalable manipulation of the mineral ensembles. With this platform, we devise several mineral-enabled dynamic surfaces and interfaces. For example, we show that the induced growth of minerals yields localized inorganic adhesion for biological tissue and reversible focal encapsulation for sensitive components in flexible electronics.
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
Authors: Gustafsen Camilla , Olsen Ditte , Vilstrup Joachim , Lund Signe , Reinhardt Anika , Wellner Niels , Larsen Torben , Andersen Christian B. F. , Weyer Kathrin , Li Jin-ping , Seeberger Peter H. , Thirup Søren , Madsen Peder , Glerup Simon 
Published: September 2017

AbstractCoronary artery disease is the main cause of death worldwide and accelerated by increased plasma levels of cholesterol-rich low-density lipoprotein particles (LDL). Circulating PCSK9 contributes to coronary artery disease by inducing lysosomal degradation of the LDL receptor (LDLR) in the liver and thereby reducing LDL clearance. Here, we show that liver heparan sulfate proteoglycans are PCSK9 receptors and essential for PCSK9-induced LDLR degradation. The heparan sulfate-binding site is located in the PCSK9 prodomain and formed by surface-exposed basic residues interacting with trisulfated heparan sulfate disaccharide repeats. Accordingly, heparan sulfate mimetics and monoclonal antibodies directed against the heparan sulfate-binding site are potent PCSK9 inhibitors. We propose that heparan sulfate proteoglycans lining the hepatocyte surface capture PCSK9 and facilitates subsequent PCSK9:LDLR complex formation. Our findings provide new insights into LDL biology and show that targeting PCSK9 using heparan sulfate mimetics is a potential therapeutic strategy in coronary artery disease.
Falling upward with Parkinson’s disease
Authors: Buetow Stephen A. , Martínez-Martín Pablo , McCormack Brendan 
Published: September 2017

AbstractFalls can injure, even kill. No one with Parkinson’s disease (PD) wants to fall by accident. However, the potential nastiness of falls does not preclude a more nuanced understanding of the personal meaning that falls can have. Rather than view falls as a problem to fear and manage solely by preventing and repairing harm, people with PD and those who care for them may recast falls as a mixed blessing. Falls may be a resource, skill, and catalyst for personal growth. We discuss how falls may give rise to opportunities in interrelated domains: capabilities, credo, character, creativity, chronemics, and connectedness. Clinicians could incorporate a positive focus across these domains to help people with PD to ‘fall upward’ in the sense of flourish.
Intense natural selection preceded the invasion of new adaptive zones during the radiation of New World leaf-nosed bats
Authors: Rossoni Daniela M. , Assis Ana Paula A. , Giannini Norberto P. , Marroig Gabriel 
Published: September 2017

AbstractThe family Phyllostomidae, which evolved in the New World during the last 30 million years, represents one of the largest and most morphologically diverse mammal families. Due to its uniquely diverse functional morphology, the phyllostomid skull is presumed to have evolved under strong directional selection; however, quantitative estimation of the strength of selection in this extraordinary lineage has not been reported. Here, we used comparative quantitative genetics approaches to elucidate the processes that drove cranial evolution in phyllostomids. We also quantified the strength of selection and explored its association with dietary transitions and specialization along the phyllostomid phylogeny. Our results suggest that natural selection was the evolutionary process responsible for cranial diversification in phyllostomid bats. Remarkably, the strongest selection in the phyllostomid phylogeny was associated with dietary specialization and the origination of novel feeding habits, suggesting that the adaptive diversification of phyllostomid bats was triggered by ecological opportunities. These findings are consistent with Simpson’s quantum evolutionary model of transitions between adaptive zones. The multivariate analyses used in this study provides a powerful tool for understanding the role of evolutionary processes in shaping phenotypic diversity in any group on both micro- and macroevolutionary scales.