bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermal tracing of retained meltwater in the lower accumulation area of the Southwestern Greenland ice sheet
Authors: Charalampidis Charalampos , Van As Dirk , Colgan William T. , Fausto Robert S. , Macferrin Michael , Machguth Horst 
Published: May 2016

ABSTRACTWe present in situ firn temperatures from the extreme 2012 melt season in the southwestern lower accumulation area of the Greenland ice sheet. The upper 2.5 m of snow and firn was temperate during the melt season, when vertical meltwater percolation was inefficient due to a ~5.5 m thick ice layer underlying the temperate firn. Meltwater percolation and refreezing beneath 2.5 m depth only occurred after the melt season. Deviations from temperatures predicted by pure conductivity suggest that meltwater refroze in discrete bands at depths of 2.0–2.5, 5.0–6.0 and 8.0–9.0 m. While we find no indication of meltwater percolation below 9 m depth or complete filling of pore volume above, firn at 10 and 15 m depth was respectively 4.2–4.5°C and 1.7°C higher than in a conductivity-only simulation. Even though meltwater percolation in 2012 was inefficient, firn between 2 and 15 m depth the following winter was on average 4.7°C warmer due to meltwater refreezing. Our observations also suggest that the 2012 firn conditions were preconditioned by two warm summers and ice layer formation in 2010 and 2011. Overall, firn temperatures during the years 2009–13 increased by 0.6°C.
Surgical repair of an aneurysm-like fistula connecting the left main coronary artery with the right atrium
Authors: Tong Guang , Sun Zhongchan , Zhang Weida 
Published: May 2016

AbstractCoronary fistula is defined as an anomalous connection between a coronary artery and any of the four chambers of the heart or any of its great vessels. A coronary fistula connecting the left main coronary artery to the right atrium is the most uncommon. In the present study, we report the surgical management of a very uncommon case of an aneurysm-like fistula connecting the left main coronary artery to the right atrium in a 2-year-old boy.
MLL1 and MLL1 fusion proteins have distinct functions in regulating leukemic transcription program
Authors: Xu Jing , Li Li , Xiong Jie , denDekker Aaron , Ye Andrew , Karatas Hacer , Liu Liu , Wang He , Qin Zhaohui S , Wang Shaomeng , Dou Yali 
Published: May 2016

AbstractMixed lineage leukemia protein-1 (MLL1) has a critical role in human MLL1 rearranged leukemia (MLLr) and is a validated therapeutic target. However, its role in regulating global gene expression in MLLr cells, as well as its interplay with MLL1 fusion proteins remains unclear. Here we show that despite shared DNA-binding and cofactor interacting domains at the N terminus, MLL1 and MLL-AF9 are recruited to distinct chromatin regions and have divergent functions in regulating the leukemic transcription program. We demonstrate that MLL1, probably through C-terminal interaction with WDR5, is recruited to regulatory enhancers that are enriched for binding sites of E-twenty-six (ETS) family transcription factors, whereas MLL-AF9 binds to chromatin regions that have no H3K4me1 enrichment. Transcriptome-wide changes induced by different small molecule inhibitors also highlight the distinct functions of MLL1 and MLL-AF9. Taken together, our studies provide novel insights on how MLL1 and MLL fusion proteins contribute to leukemic gene expression, which have implications for developing effective therapies in the future.
Biogeography of sulfur-oxidizing Acidithiobacillus populations in extremely acidic cave biofilms
Authors: Jones Daniel S , Schaperdoth Irene , Macalady Jennifer L 
Published: May 2016

Abstract
Extremely acidic (pH 0–1.5) Acidithiobacillus-dominated biofilms known as snottites are found in sulfide-rich caves around the world. Given the extreme geochemistry and subsurface location of the biofilms, we hypothesized that snottite Acidithiobacillus populations would be genetically isolated. We therefore investigated biogeographic relationships among snottite Acidithiobacillus spp. separated by geographic distances ranging from meters to 1000s of kilometers. We determined genetic relationships among the populations using techniques with three levels of resolution: (i) 16S rRNA gene sequencing, (ii) 16S–23S intergenic transcribed spacer (ITS) region sequencing and (iii) multi-locus sequencing typing (MLST). We also used metagenomics to compare functional gene characteristics of select populations. Based on 16S rRNA genes, snottites in Italy and Mexico are dominated by different sulfur-oxidizing Acidithiobacillus spp. Based on ITS sequences, Acidithiobacillus thiooxidans strains from different cave systems in Italy are genetically distinct. Based on MLST of isolates from Italy, genetic distance is positively correlated with geographic distance both among and within caves. However, metagenomics revealed that At. thiooxidans populations from different cave systems in Italy have different sulfur oxidation pathways and potentially other significant differences in metabolic capabilities. In light of those genomic differences, we argue that the observed correlation between genetic and geographic distance among snottite Acidithiobacillus populations is partially explained by an evolutionary model in which separate cave systems were stochastically colonized by different ancestral surface populations, which then continued to diverge and adapt in situ.
Physiological constraints to climate warming in fish follow principles of plastic floors and concrete ceilings
Authors: Sandblom Erik , Clark Timothy D. , Gräns Albin , Ekström Andreas , Brijs Jeroen , Sundström L. Fredrik , Odelström Anne , Adill Anders , Aho Teija , Jutfelt Fredrik 
Published: May 2016

AbstractUnderstanding the resilience of aquatic ectothermic animals to climate warming has been hindered by the absence of experimental systems experiencing warming across relevant timescales (for example, decades). Here, we examine European perch (Perca fluviatilis, L.) from the Biotest enclosure, a unique coastal ecosystem that maintains natural thermal fluctuations but has been warmed by 5–10 °C by a nuclear power plant for over three decades. We show that Biotest perch grow faster and display thermally compensated resting cardiorespiratory functions compared with reference perch living at natural temperatures in adjacent waters. However, maximum cardiorespiratory capacities and heat tolerance limits exhibit limited or no thermal compensation when compared with acutely heated reference perch. We propose that while basal energy requirements and resting cardiorespiratory functions (floors) are thermally plastic, maximum capacities and upper critical heat limits (ceilings) are much less flexible and thus will limit the adaptive capacity of fishes in a warming climate.
Decimetre-scale multicellular eukaryotes from the 1.56-billion-year-old Gaoyuzhuang Formation in North China
Authors: Zhu Shixing , Zhu Maoyan , Knoll Andrew H. , Yin Zongjun , Zhao Fangchen , Sun Shufen , Qu Yuangao , Shi Min , Liu Huan 
Published: May 2016

AbstractFossils of macroscopic eukaryotes are rarely older than the Ediacaran Period (635–541 million years (Myr)), and their interpretation remains controversial. Here, we report the discovery of macroscopic fossils from the 1,560-Myr-old Gaoyuzhuang Formation, Yanshan area, North China, that exhibit both large size and regular morphology. Preserved as carbonaceous compressions, the Gaoyuzhuang fossils have statistically regular linear to lanceolate shapes up to 30 cm long and nearly 8 cm wide, suggesting that the Gaoyuzhuang fossils record benthic multicellular eukaryotes of unprecedentedly large size. Syngenetic fragments showing closely packed ∼10 μm cells arranged in a thick sheet further reinforce the interpretation. Comparisons with living thalloid organisms suggest that these organisms were photosynthetic, although their phylogenetic placement within the Eukarya remains uncertain. The new fossils provide the strongest evidence yet that multicellular eukaryotes with decimetric dimensions and a regular developmental program populated the marine biosphere at least a billion years before the Cambrian Explosion.
Negative magnetoresistance without well-defined chirality in the Weyl semimetal TaP
Authors: Arnold Frank , Shekhar Chandra , Wu Shu-Chun , Sun Yan , dos Reis Ricardo Donizeth , Kumar Nitesh , Naumann Marcel , Ajeesh Mukkattu O. , Schmidt Marcus , Grushin Adolfo G. , Bardarson Jens H. , Baenitz Michael , Sokolov Dmitry , Borrmann Horst , Nicklas Michael , Felser Claudia , Hassinger Elena , Yan Binghai 
Published: May 2016

AbstractWeyl semimetals (WSMs) are topological quantum states wherein the electronic bands disperse linearly around pairs of nodes with fixed chirality, the Weyl points. In WSMs, nonorthogonal electric and magnetic fields induce an exotic phenomenon known as the chiral anomaly, resulting in an unconventional negative longitudinal magnetoresistance, the chiral-magnetic effect. However, it remains an open question to which extent this effect survives when chirality is not well-defined. Here, we establish the detailed Fermi-surface topology of the recently identified WSM TaP via combined angle-resolved quantum-oscillation spectra and band-structure calculations. The Fermi surface forms banana-shaped electron and hole pockets surrounding pairs of Weyl points. Although this means that chirality is ill-defined in TaP, we observe a large negative longitudinal magnetoresistance. We show that the magnetoresistance can be affected by a magnetic field-induced inhomogeneous current distribution inside the sample.
Uniaxial ferroelectric quantum criticality in multiferroic hexaferrites BaFe12O19 and SrFe12O19
Authors: Rowley S. E. , Chai Yi-Sheng , Shen Shi-Peng , Sun Young , Jones A. T. , Watts B. E. , Scott J. F. 
Published: May 2016

AbstractBaFe12O19 is a popular M-type hexaferrite with a Néel temperature of 720 K and is of enormous commercial value ($3 billion/year). It is an incipient ferroelectric with an expected ferroelectric phase transition extrapolated to lie at 6 K but suppressed due to quantum fluctuations. The theory of quantum criticality for such uniaxial ferroelectrics predicts that the temperature dependence of the electric susceptibility χ diverges as 1/T3, in contrast to the 1/T2 dependence found in pseudo-cubic materials such as SrTiO3 or KTaO3. In this paper we present evidence of the susceptibility varying as 1/T3, i.e. with a critical exponent γ = 3. In general γ = (d + z – 2)/z, where the dynamical exponent for a ferroelectric z = 1 and the dimension is increased by 1 from deff = 3 + z to deff = 4 + z due to the effect of long-range dipole interactions in uniaxial as opposed to multiaxial ferroelectrics. The electric susceptibility of the incipient ferroelectric SrFe12O19, which is slightly further from the quantum phase transition is also found to vary as 1/T3.
Hyperosmosis and its combination with nutrient-limitation are novel environmental stressors for induction of triacylglycerol accumulation in cells of Chlorella kessleri
Authors: Hirai Kazuho , Hayashi Taihei , Hasegawa Yuri , Sato Atsushi , Tsuzuki Mikio , Sato Norihiro 
Published: May 2016

AbstractTriacylglycerols of oleaginous algae are promising for production of food oils and biodiesel fuel. Air-drying of cells induces triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, therefore, it seems that dehydration, i.e., intracellular hyperosmosis, and/or nutrient-limitation are key stressors. We explored this possibility in liquid-culturing C. kessleri cells. Strong hyperosmosis with 0.9 M sorbitol or 0.45 M NaCl for two days caused cells to increase the triacylglycerol content in total lipids from 1.5 to 48.5 and 75.3 mol%, respectively, on a fatty acid basis, whereas nutrient-limitation caused its accumulation to 41.4 mol%. Even weak hyperosmosis with 0.3 M sorbitol or 0.15 M NaCl, when nutrient-limitation was simultaneously imposed, induced triacylglycerol accumulation to 61.9 and 65.7 mol%, respectively. Furthermore, culturing in three-fold diluted seawater, the chemical composition of which resembled that of the medium for the combinatory stress, enabled the cells to accumulate triacylglycerol up to 24.7 weight% of dry cells in only three days. Consequently, it was found that hyperosmosis is a novel stressor for triacylglycerol accumulation, and that weak hyperosmosis, together with nutrient-limitation, exerts a strong stimulating effect on triacylglycerol accumulation. A similar combinatory stress would contribute to the triacylglycerol accumulation in air-dried C. kessleri cells.
Catheter-based Intramyocardial Injection of FGF1 or NRG1-loaded MPs Improves Cardiac Function in a Preclinical Model of Ischemia-Reperfusion
Authors: Garbayo Elisa , Gavira Juan José , de Yebenes Manuel Garcia , Pelacho Beatriz , Abizanda Gloria , Lana Hugo , Blanco-Prieto María José , Prosper Felipe 
Published: May 2016

AbstractCardiovascular protein therapeutics such as neuregulin (NRG1) and acidic-fibroblast growth factor (FGF1) requires new formulation strategies that allow for sustained bioavailability of the drug in the infarcted myocardium. However, there is no FDA-approved injectable protein delivery platform due to translational concerns about biomaterial administration through cardiac catheters. We therefore sought to evaluate the efficacy of percutaneous intramyocardial injection of poly(lactic-co-glycolic acid) microparticles (MPs) loaded with NRG1 and FGF1 using the NOGA MYOSTAR injection catheter in a porcine model of ischemia-reperfusion. NRG1- and FGF1-loaded MPs were prepared using a multiple emulsion solvent-evaporation technique. Infarcted pigs were treated one week after ischemia-reperfusion with MPs containing NRG1, FGF1 or non-loaded MPs delivered via clinically-translatable percutaneous transendocardial-injection. Three months post-treatment, echocardiography indicated a significant improvement in systolic and diastolic cardiac function. Moreover, improvement in bipolar voltage and decrease in transmural infarct progression was demonstrated by electromechanical NOGA-mapping. Functional benefit was associated with an increase in myocardial vascularization and remodeling. These findings in a large animal model of ischemia-reperfusion demonstrate the feasibility and efficacy of using MPs as a delivery system for growth factors and provide strong evidence to move forward with clinical studies using therapeutic proteins combined with catheter-compatible biomaterials.
Correlative near-infrared light and cathodoluminescence microscopy using Y2O3:Ln, Yb (Ln = Tm, Er) nanophosphors for multiscale, multicolour bioimaging
Authors: Fukushima S. , Furukawa T. , Niioka H. , Ichimiya M. , Sannomiya T. , Tanaka N. , Onoshima D. , Yukawa H. , Baba Y. , Ashida M. , Miyake J. , Araki T. , Hashimoto M. 
Published: May 2016

AbstractThis paper presents a new correlative bioimaging technique using Y2O3:Tm, Yb and Y2O3:Er, Yb nanophosphors (NPs) as imaging probes that emit luminescence excited by both near-infrared (NIR) light and an electron beam. Under 980 nm NIR light irradiation, the Y2O3:Tm, Yb and Y2O3:Er, Yb NPs emitted NIR luminescence (NIRL) around 810 nm and 1530 nm, respectively and cathodoluminescence at 455 nm and 660 nm under excitation of accelerated electrons, respectively. Multimodalities of the NPs were confirmed in correlative NIRL/CL imaging and their locations were visualized at the same observation area in both NIRL and CL images. Using CL microscopy, the NPs were visualized at the single-particle level and with multicolour. Multiscale NIRL/CL bioimaging was demonstrated through in vivo and in vitro NIRL deep-tissue observations, cellular NIRL imaging and high-spatial resolution CL imaging of the NPs inside cells. The location of a cell sheet transplanted onto the back muscle fascia of a hairy rat was visualized through NIRL imaging of the Y2O3:Er, Yb NPs. Accurate positions of cells through the thickness (1.5 mm) of a tissue phantom were detected by NIRL from the Y2O3:Tm, Yb NPs. Further, locations of the two types of NPs inside cells were observed using CL microscopy.
Crystalline-Amorphous-Crystalline Transformation in a Highly Brilliant Luminescent System with Trigonal-Planar Gold(I) Centers
Authors: Igawa Kosuke , Yoshinari Nobuto , Okumura Mitsutaka , Ohtsu Hiroyoshi , Kawano Masaki , Konno Takumi 
Published: May 2016

AbstractPhotoluminescent compounds showing emission color changes in response to external stimuli have received considerable attention because of their wide range of applications. Here, we report the unique photoluminescence behavior of a digold(I) coordination system with trigonal-planar AuI centers, [Au2(dppm)3]2+ (dppm = bis(diphenylphosphino)methane). This system shows an extremely intense phosphorescence, with a quantum yield of >95% in the solid state. Both the emission color and thermal stability vary due to changing counter ions (Cl− vs. OTf−). Of particular note is the thermal crystalline-amorphous-crystalline transformation for the chloride salt, which is accompanied by drastic emission color changes. Single-crystal and powder X-ray diffractions demonstrate that the two-step transformation is induced by the loss of water molecules of crystallization with the subsequent removal of a dppm ligand to form [Au2(dppm)2]2+, which is mechanically reverted back to [Au2(dppm)3]2+.
Revealing martensitic transformation and α/β interface evolution in electron beam melting three-dimensional-printed Ti-6Al-4V
Authors: Tan Xipeng , Kok Yihong , Toh Wei Quan , Tan Yu Jun , Descoins Marion , Mangelinck Dominique , Tor Shu Beng , Leong Kah Fai , Chua Chee Kai 
Published: May 2016

AbstractAs an important metal three-dimensional printing technology, electron beam melting (EBM) is gaining increasing attention due to its huge potential applications in aerospace and biomedical fields. EBM processing of Ti-6Al-4V as well as its microstructure and mechanical properties were extensively investigated. However, it is still lack of quantitative studies regarding its microstructural evolution, indicative of EBM thermal process. Here, we report α′ martensitic transformation and α/β interface evolution in varied printing thicknesses of EBM-printed Ti-6Al-4V block samples by means of atom probe tomography. Quantitative chemical composition analysis suggests a general phase transformation sequence. By increasing in-fill hatched thickness, elemental partitioning ratios arise and β volume fraction is increased. Furthermore, we observe kinetic vanadium segregation and aluminum depletion at interface front and the resultant α/β interface widening phenomenon. It may give rise to an increased α/β lattice mismatch and weakened α/β interfaces, which could account for the degraded strength as printing thickness increases.
Differential investment in visual and olfactory brain areas reflects behavioural choices in hawk moths
Authors: Stöckl Anna , Heinze Stanley , Charalabidis Alice , el Jundi Basil , Warrant Eric , Kelber Almut 
Published: May 2016

AbstractNervous tissue is one of the most metabolically expensive animal tissues, thus evolutionary investments that result in enlarged brain regions should also result in improved behavioural performance. Indeed, large-scale comparative studies in vertebrates and invertebrates have successfully linked differences in brain anatomy to differences in ecology and behaviour, but their precision can be limited by the detail of the anatomical measurements, or by only measuring behaviour indirectly. Therefore, detailed case studies are valuable complements to these investigations and have provided important evidence linking brain structure to function in a range of higher-order behavioural traits, such as foraging experience or aggressive behaviour. Here, we show that differences in the size of both lower and higher-order sensory brain areas reflect differences in the relative importance of these senses in the foraging choices of hawk moths, as suggested by previous anatomical work in Lepidopterans. To this end we combined anatomical and behavioural quantifications of the relative importance of vision and olfaction in two closely related hawk moth species. We conclude that differences in sensory brain volume in these hawk moths can indeed be interpreted as differences in the importance of these senses for the animal’s behaviour.
Synergetic enhancement of gold nanoparticles and 2-mercaptobenzothiazole as highly-sensitive sensing strategy for tetrabromobisphenol A
Authors: Chen Xuerong , Ji Liudi , Zhou Yikai , Wu Kangbing 
Published: May 2016

AbstractVarious gold nanoparticles (AuNPs) were in-situ prepared on the electrode surface through electrochemical reduction under different potentials such as −0.60, −0.50, −0.40, −0.30 and −0.20 V. The reduction potentials heavily affect the surface morphology and electrochemical activity of AuNPs such as effective area and catalytic ability, as confirmed using atomic force microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors of tetrabromobisphenol A (TBBPA), a widely-existed pollutant with severe adverse health effects, were studied. The oxidation activity of TBBPA enhances obviously on the surface of AuNPs and the signal improvements of TBBPA show difference on the prepared AuNPs. Interestingly, the existence of 2-mercaptobenzothiazole (MBT) further improves the oxidation signals of TBBPA on AuNPs. The synergetic enhancement effects of AuNPs and MBT were studied using cyclic voltammetry and chronocoulometry. The numerous nano-scaled gold particles together with the strong hydrophobic interaction between TBBPA and the assembled MBT on AuNPs jointly provide highly-effective accumulation for TBBPA. As a result, a sensitive and simple electrochemical method was developed for the direct determination of TBBPA, with detection limit of 0.12 μg L−1 (0.22 nM). The practical applications in water samples manifest that this new sensing system is accurate and feasible.
Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor
Authors: Brandstätter Olga , Schanz Oliver , Vorac Julia , König Jessica , Mori Tetsushi , Maruyama Toru , Korkowski Markus , Haarmann-Stemmann Thomas , von Smolinski Dorthe , Schultze Joachim L. , Abel Josef , Esser Charlotte , Takeyama Haruko , Weighardt Heike , Förster Irmgard 
Published: May 2016

AbstractAs a sensor of polyaromatic chemicals the aryl hydrocarbon receptor (AhR) exerts an important role in immune regulation besides its requirement for xenobiotic metabolism. Transcriptional activation of AhR target genes is counterregulated by the AhR repressor (AhRR) but the exact function of the AhRR in vivo is currently unknown. We here show that the AhRR is predominantly expressed in immune cells of the skin and intestine, different from other AhR target genes. Whereas AhRR antagonizes the anti-inflammatory function of the AhR in the context of systemic endotoxin shock, AhR and AhRR act in concert to dampen intestinal inflammation. Specifically, AhRR contributes to the maintenance of colonic intraepithelial lymphocytes and prevents excessive IL-1β production and Th17/Tc17 differentiation. In contrast, the AhRR enhances IFN-γ-production by effector T cells in the inflamed gut. Our findings highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli.