bluuebunny/crossref_metadata_2025_split huggingface dataset:
Central nervous system involvement in acute lymphoblastic leukemia is mediated by vascular endothelial growth factor
Authors: Münch Vera , Trentin Luca , Herzig Julia , Demir Salih , Seyfried Felix , Kraus Johann M. , Kestler Hans A. , Köhler Rolf , Barth Thomas F. E. , te Kronnie Geertruy , Debatin Klaus-Michael , Meyer Lüder H. 
Published: May 2017

Key Points
Xenografted ALL cells faithfully recapitulate CNS leukemia and are characterized by high expression of VEGF, mediating CNS entry of ALL cells. VEGF captured by bevacizumab in vivo specifically reduces CNS leukemia, providing a novel strategy to target CNS involvement in ALL.
Essential role for histone deacetylase 11 (HDAC11) in neutrophil biology
Authors: Sahakian Eva , Chen Jie , Powers John J , Chen Xianghong , Maharaj Kamira , Deng Susan L , Achille Alex N , Lienlaf Maritza , Wang Hong Wei , Cheng Fengdong , Sodré Andressa L , Distler Allison , Xing Limin , Perez-Villarroel Patricio , Wei Sheng , Villagra Alejandro , Seto Ed , Sotomayor Eduardo M , Horna Pedro , Pinilla-Ibarz Javier 
Published: May 2017

Abstract
Epigenetic changes in chromatin structure have been recently associated with the deregulated expression of critical genes in normal and malignant processes. HDAC11, the newest member of the HDAC family of enzymes, functions as a negative regulator of IL-10 expression in APCs, as previously described by our lab. However, at the present time, its role in other hematopoietic cells, specifically in neutrophils, has not been fully explored. In this report, for the first time, we present a novel physiologic role for HDAC11 as a multifaceted regulator of neutrophils. Thus far, we have been able to demonstrate a lineage-restricted overexpression of HDAC11 in neutrophils and committed neutrophil precursors (promyelocytes). Additionally, we show that HDAC11 appears to associate with the transcription machinery, possibly regulating the expression of inflammatory and migratory genes in neutrophils. Given the prevalence of neutrophils in the peripheral circulation and their central role in the first line of defense, our results highlight a unique and novel role for HDAC11. With the consideration of the emergence of new, selective HDAC11 inhibitors, we believe that our findings will have significant implications in a wide range of diseases spanning malignancies, autoimmunity, and inflammation.
Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages
Authors: Bzowska Małgorzata , Nogieć Anna , Bania Krystian , Zygmunt Magdalena , Zarębski Mirosław , Dobrucki Jerzy , Guzik Krzysztof 
Published: May 2017

Abstract
Heat shock proteins (HSPs) are typical intracellular chaperones which also appear on the cell surface and in extracellular milieu. HSP90, which chaperones many proteins involved in signal transduction, is also a regular component of LPS-signaling complexes on Mϕ. As LPS is a prototypical PAMP, we speculated that HSP90 is engaged in pattern recognition by professional phagocytes. In this report, we provide the first evidence, to our knowledge, of the geldanamycin (Ge)-inhibitable HSP90 on the surface of live monocyte-derived Mϕs (hMDMs). Using cytometry and specific Abs, we showed both HSP90 isoforms (α and β) on the surface of human monocytes and hMDMs. The cell-surface HSP90 pool was also labeled with cell-impermeable Ge derivatives. Confocal analysis of hMDMs revealed that HSP90-inhibitor complexes were rapidly clustered on the cell surface and recycled through the endosomal compartment. This finding suggests that the N-terminal (ATPase) domain of HSP90 is exposed and accessible from the extracellular space. To study the role of cell-surface HSP90 in pattern recognition, we used pathogen (PAMPs)- or apoptotic cell-associated molecular patterns (ACAMPs). We showed that blocking the cell-surface HSP90 pool leads to a dramatic decrease in TNF production by monocytes and hMDMs exposed to soluble (TLRs-specific ligands) and particulate [bacteria Staphylococcus aureus (SA) and Porphyromonas gingivalis (PG)] PAMPs. Surprisingly, in hMDMs the functional cell-surface HSP90 was not necessary for the engulfment of either apoptotic neutrophils or bacteria. The presented data suggest that the cell-surface HSP90 is a “signaling complex chaperone,” with activity that is essential for cytokine response but not for target engulfment by Mϕ.
The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis
Authors: Dougher Christopher W L , Buffone Alexander , Nemeth Michael J , Nasirikenari Mehrab , Irons Eric E , Bogner Paul N , Lau Joseph T Y 
Published: May 2017

Abstract
Responding to systemic demands in producing and replenishing end-effector blood cells is predicated on the appropriate delivery and interpretation of extrinsic signals to the HSPCs. The data presented herein implicate the systemic, extracellular form of the glycosyltransferase ST6Gal-1 in the regulation of late-stage neutrophil development. ST6Gal-1 is typically a membrane-bound enzyme sequestered within the intracellular secretory apparatus, but an extracellular form is released into the blood from the liver. Both human and murine HSPCs, upon exposure to extracellular ST6Gal-1 ex vivo, exhibited decreased proliferation, diminished expression of the neutrophilic primary granule protein MPO, and decreased appearance of CD11b+ cells. HSPC suppression was preceded by decreased STAT-3 phosphorylation and diminished C/EBPα expression, without increased apoptosis, indicating attenuated G-CSF receptor signaling. A murine model to raise systemic ST6Gal-1 level was developed to examine the role of the circulatory enzyme in vivo. Our results show that systemic ST6Gal-1 modified the cell surface of the GMP subset of HSPCs and decreased marrow neutrophil reserves. Acute airway neutrophilic inflammation by LPS challenge was used to drive demand for new neutrophil production. Reduced neutrophil infiltration into the airway was observed in mice with elevated circulatory ST6Gal-1 levels. The blunted transition of GMPs into GPs in vitro is consistent with ST6Gal-1-attenuated granulopoiesis. The data confirm that circulatory ST6Gal-1 is a negative systemic regulator of granulopoiesis and moreover suggest a clinical potential to limit the number of inflammatory cells by manipulating blood ST6Gal-1 levels.
Francisella novicida inhibits spontaneous apoptosis and extends human neutrophil lifespan
Authors: Kinkead Lauren C , Fayram Drew C , Allen Lee-Ann H 
Published: May 2017

Abstract
Francisella novicida is a Gram-negative bacterium that is closely related to the highly virulent facultative intracellular pathogen, Francisella tularensis. Data published by us and others demonstrate that F. tularensis virulence correlates directly with its ability to impair constitutive apoptosis and extend human neutrophil lifespan. In contrast, F. novicida is attenuated in humans, and the mechanisms that account for this are incompletely defined. Our published data demonstrate that F. novicida binds natural IgG that is present in normal human serum, which in turn, elicits NADPH oxidase activation that does not occur in response to F. tularensis. As it is established that phagocytosis and oxidant production markedly accelerate neutrophil death, we predicted that F. novicida may influence the neutrophil lifespan in an opsonin-dependent manner. To test this hypothesis, we quantified bacterial uptake, phosphatidylserine (PS) externalization, and changes in nuclear morphology, as well as the kinetics of procaspase-3, -8, and -9 processing and activation. To our surprise, we discovered that F. novicida not only failed to accelerate neutrophil death but also diminished and delayed apoptosis in a dose-dependent, but opsonin-independent, manner. In keeping with this, studies of conditioned media (CM) showed that neutrophil longevity could be uncoupled from phagocytosis and that F. novicida stimulated neutrophil secretion of CXCL8. Taken together, the results of this study reveal shared and unique aspects of the mechanisms used by Francisella species to manipulate neutrophil lifespan and as such, advance understanding of cell death regulation during infection.
Myeloid-derived cells in prostate cancer progression: phenotype and prospective therapies
Authors: Lopez-Bujanda Zoila , Drake Charles G 
Published: May 2017

AbstractProstate cancer is the second most common cause of cancer mortality in men in the United States. As is the case for other tumor types, accumulating evidence suggests an important role for myeloid-derived cells in the promotion and progression of prostate cancer. Here, we briefly describe myeloid-derived cells that interact with tumor cells and what is known about their immune suppressive function. We next discuss new evidence for tumor cell–mediated myeloid infiltration via the PI3K/PTEN/AKT signaling pathway and an alternative mechanism for immune evasion that may be regulated by an endoplasmic reticulum stress response. Finally, we discuss several interventions that target myeloid-derived cells to treat prostate cancer.
Comparative exploration of multidimensional flow cytometry software: a model approach evaluating T cell polyfunctional behavior
Authors: Spear Timothy T , Nishimura Michael I , Simms Patricia E 
Published: May 2017

Abstract
Advancement in flow cytometry reagents and instrumentation has allowed for simultaneous analysis of large numbers of lineage/functional immune cell markers. Highly complex datasets generated by polychromatic flow cytometry require proper analytical software to answer investigators’ questions. A problem among many investigators and flow cytometry Shared Resource Laboratories (SRLs), including our own, is a lack of access to a flow cytometry-knowledgeable bioinformatics team, making it difficult to learn and choose appropriate analysis tool(s). Here, we comparatively assess various multidimensional flow cytometry software packages for their ability to answer a specific biologic question and provide graphical representation output suitable for publication, as well as their ease of use and cost. We assessed polyfunctional potential of TCR-transduced T cells, serving as a model evaluation, using multidimensional flow cytometry to analyze 6 intracellular cytokines and degranulation on a per-cell basis. Analysis of 7 parameters resulted in 128 possible combinations of positivity/negativity, far too complex for basic flow cytometry software to analyze fully. Various software packages were used, analysis methods used in each described, and representative output displayed. Of the tools investigated, automated classification of cellular expression by nonlinear stochastic embedding (ACCENSE) and coupled analysis in Pestle/simplified presentation of incredibly complex evaluations (SPICE) provided the most user-friendly manipulations and readable output, evaluating effects of altered antigen-specific stimulation on T cell polyfunctionality. This detailed approach may serve as a model for other investigators/SRLs in selecting the most appropriate software to analyze complex flow cytometry datasets. Further development and awareness of available tools will help guide proper data analysis to answer difficult biologic questions arising from incredibly complex datasets.
A micro ocean-bottom E-field receiver
Authors: Chen Kai , Deng Ming , Luo Xianhu , Wu Zhongliang 
Published: May 2017

Ocean-bottom electromagnetic (EM) receivers are used to record EM signals for marine magnetotelluric and controlled-source EM offshore data acquisition. These marine EM data are used for offshore gas hydrate and petroleum exploration. Although many conventional receivers are used for offshore data acquisition, they have deficiencies, such as a large size, high cost, and low operational efficiency. To address these limitations, we have developed a micro ocean-bottom E-field (micro-OBE) receiver. It reduces costs and deck-space use, while providing improved horizontal resolution and operational efficiency. Based on conventional receiver specifications, including low noise levels, low power consumption, and low clock-drift error, we reduced the receiver size, provided a low-cost release mechanism, integrated an acoustic telemetry module, improved the operational efficiency, and reduced the field acquisition cost. The resulting micro-OBE is comprised of a compact nylon frame, 17 inch glass sphere, logging system, batteries, recovery beacon, burn-wire release mechanism, transducer, electrode, red flag, and anchor (which eliminates the heavy and expensive acoustic release). It has no alloy aluminum press case, and it contains a minimal number of glass spheres, watertight cables, and connectors. Its size is [Formula: see text] (not including the electrode arm, red flag, and anchor). Its weight in air is 70 kg. It has a noise level of [Formula: see text] (Hz) at 8 Hz, and it provides 33 days of battery lifetime. Offshore data acquisition was performed to evaluate the micro-OBE field performance during an offshore gas-hydrate experiment. Our results indicate that the receiver effectively functioned throughout the operation and offshore data acquisition. Micro-OBE was thus verified as providing a low cost, compact size, and high operational efficiency.
A novel experimental rat model of peripheral nerve scarring: reliably mimicking post-surgical complications and recurring adhesions
Authors: Lemke Angela , Penzenstadler Carina , Ferguson James , Lidinsky Dominika , Hopf Rudolf , Bradl Monika , Redl Heinz , Wolbank Susanne , Hausner Thomas 
Published: May 2017

Inflammation, fibrosis and perineural adhesions with the surrounding tissue are common pathological processes following nerve injury and surgical interventions on peripheral nerves in human patients. Furthermore, these signs can reoccur following external neurolysis, currently the most common surgical treatment, thus leading to renewed nerve function impairment and chronic pain. To enable a successful evaluation of new therapeutic approaches, it is crucial to use a reproducible animal model that mimics the main clinical symptoms occurring in human patients. However, a clinically relevant model combining both histological and functional alterations has not been published to date. For this reason, we developed a reliable rat model, which exhibits the essential pathological processes of peripheral nerve scarring.
In our study, we present a novel method for the induction of nerve scarring by applying glutaraldehyde-containing glue, known to cause nerve injury in humans. After a three-week contact period with the sciatic nerve in female Sprague Dawley rats, we could demonstrate severe intra- and perineural scarring that resulted in grade 3 adhesions and major impairments in the electrophysiological peak amplitude compared to sham control (p=0.0478). Immunohistochemical analysis of the nerve structure revealed vigorous nerve inflammation and recruitment of T cells and macrophages. Also, distinct nerve degeneration was determined by immunostaining. These pathological alterations were further reflected in significant functional deficiencies as determined by the analysis of relevant gait parameters as well as the quantification of the sciatic functional index starting at week 1 post-operatively (p&lt;0.01). Moreover, with this model we could, for the first time, demonstrate not only the primary formation, but also the recurrence of severe adhesions one week after glue removal, imitating a major clinical challenge. As a comparison, we tested an already published model for generating perineural fibrotic adhesions, which however did not result in significant pathological changes.
Taken together, we established an easily reproducible and reliable rat model for peripheral nerve scarring which allows for the effective testing of new therapeutic strategies.
The Notion of the Diameter of Mereological Ball in Tarski's Geometry of Solids
Authors: Sitek Grzegorz 
Published: May 2017

In the paper "Full development of Tarski's geometry of solids" Gruszczyński and Pietruszczak have obtained the full development of Tarski’s geometry of solids that was sketched in [14, 15]. In this paper 1 we introduce in Tarski’s theory the notion of congruence of mereological balls and then the notion of diameter of mereological ball. We prove many facts about these new concepts, e.g., we give a characterization of mereological balls in terms of its center and diameter and we prove that the set of all diameters together with the relation of inequality of diameters is the dense linearly ordered set without the least and the greatest element.
RELEVANCE OF THE COMPETITIVE INTELLIGENCE PROCESS ON THE SPANISH PHARMACEUTICAL COMPANIES
Authors: Fernández-Arias M. Paula , Quevedo-Cano Pilar , Hidalgo Antonio 
Published: May 2017

It is common to find Competitive Intelligence activities within the high-tech enterprises in particular in the pharmaceutical industry. These companies not only use the process of Competitive Intelligence to act against competitors, traditional aim of the Competitive Intelligence. It is increasingly used to enhance cooperation. The Cycle of CI Competitive Intelligence is used both as to extract offensive and defensive intelligence as cooperative intelligence. The paper presents the results of a sample study of 186 Spanish pharmaceutical companies that were asked about the purpose of cooperation in their Competitive Intelligence activities. The results confirm that these are used in the development of business relationships, search for partners, joint research, etc. and that companies bet on these relationships for the future within the Competitive Intelligence.
POWERING CUSTOMER CONNECTIVITY OF PRODUCTS WITH LAST MILE SUPPORT SERVICES: EVIDENCES FROM INDIAN PASSENGER CAR MANUFACTURING
Authors: Jasial Sumeet Singh , Pal S S , Bansal Sanjeev 
Published: May 2017

The paper highlights post-production customer connectivity processes in an automobile manufacturing set-up. Identity of post-production processes has been established as a unique in company service operations or pseudo-manufacturing activities. Consequently this research observes that handling these activities purely with a manufacturing focus or with a pure service focus would not be that effective. This paper in-turn proposes a new approach to powering the customer connectivity process in the last mile of a product’s journey from manufacturing to market. The scenario has been addressed in three domains, viz., Post-Production pre-market functions, Product Installation, and Product Liabilities. Through a composite process map of the post-production situations it captures the order winning elements to pick up opportunities for the possible reinforcement of adding value for money in the last mile activities on the product from company to customer.
USING THE SERVQUAL MODEL TO ASSESSMALL SERVICE QUALITY AND CUSTOMER SATISFACTION
Authors: Falcão Larissa Maria Argollo de Arruda , Jerônimo Taciana de Barros , Melo Fagner José Coutinho de , Aquino Joas Tomaz de , Medeiros Denise Dumke de 
Published: May 2017

This paper use SERVQUAL measure for assessingthe quality of the administrative services in a large mall,located in Brazil, seen from the point of view of the currentservice providers. A questionnaire was used to interview 68service companies, and 5 CEO mall of this case of study. Twofactors were compared: the expectations and perceptions of theservice providers, the comparison enabled the strengths andweaknesses of the quality perceived of the services offered bythe mall were undertaken as a case study. Understand whatfeatures and level of service must have in order to meetconsumer needs, against on how consumers perceive the actualmall performance in the context of what they expected to ensuring good service to classify into three categories offactors: Basic, Performance and Excitement.The findings ofthis study may prompt future research to create a new tool tohelp those managers and service companies in the process ofmaking decisions aimed at improving the strategic relationshipwith retail companies and thus to improve the quality of themall services. One contribution of this study is the simpleproposed model used to understanding how quality processesaffect each organizational dimension of service performance.
Jumping Performance is Preserved but Not Muscle Thickness in Collegiate Volleyball Players After a Taper
Authors: Bazyler Caleb D. , Mizuguchi Satoshi , Sole Christopher J. , Suchomel Timothy J. , Sato Kimitake , Kavanaugh Ashley A. , DeWeese Brad H. , Stone Michael H. 
Published: May 2017

AbstractBazyler, CD, Mizuguchi, S, Sole, CJ, Suchomel, TJ, Sato, K, Kavanaugh, AA, DeWeese, BH, and Stone, MH. Jumping performance is preserved but not muscle thickness in collegiate volleyball players after a taper.J Strength Cond Res32(4): 1020–1028, 2018—The purpose of this study was to examine changes in muscle architecture and jumping performance in NCAA division I women's volleyball players throughout a competitive season and in preparation for conference championships. Ten women volleyball players were tested at preseason (T1), pretaper (T2), and post-taper (T3) on measures of vastus lateralis muscle thickness (MT), pennation angle (PA) and fascicle length (FL) using ultrasonography, and unloaded and loaded squat jump height (SJH) and peak power allometrically scaled to body mass (SJPPa) on a force platform. Rating of perceived exertion training load and strength training volume load were monitored weekly. Player's MT (p< 0.001, Glass's Δ = 2.8) and PA increased (p= 0.02, Δ = 3.9) after in-season training. However, MT decreased after the taper (p= 0.01, Δ = 0.6) but remained elevated above preseason values (p< 0.001, Δ = 1.7). There were no statistical changes in FL, SJH, or SJPPa. Large-to-very large negative relationships (r= −0.51 to −0.81) were observed between preseason relative maximal strength and changes in SJH and SJPPa with various loads over the season. These findings demonstrate that relatively low volumes of strength training and concurrent sport training during a tapering period are capable of preserving jumping performance, but not MT in women's volleyball players; however, jumping performance changes seem to be related to the player's strength level. Stronger players may benefit from an overreaching microcycle before the taper to preserve previously accrued muscular adaptations and jumping performance.