bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
Authors: Ellsworth Darrell L. , Blackburn Heather L. , Shriver Craig D. , Rabizadeh Shahrooz , Soon‐Shiong Patrick , Ellsworth Rachel E. 
Published: April 2017

AbstractExtensive genomic and transcriptomic heterogeneity in human cancer often negatively impacts treatment efficacy and survival, thus posing a significant ongoing challenge for modern treatment regimens. State‐of‐the‐art DNA‐ and RNA‐sequencing methods now provide high‐resolution genomic and gene expression portraits of individual cells, facilitating the study of complex molecular heterogeneity in cancer. Important developments in single‐cell sequencing (SCS) technologies over the past 5 years provide numerous advantages over traditional sequencing methods for understanding the complexity of carcinogenesis, but significant hurdles must be overcome before SCS can be clinically useful. In this review, we: (1) highlight current methodologies and recent technological advances for isolating single cells, single‐cell whole‐genome and whole‐transcriptome amplification using minute amounts of nucleic acids, and SCS, (2) summarize research investigating molecular heterogeneity at the genomic and transcriptomic levels and how this heterogeneity affects clonal evolution and metastasis, and (3) discuss the promise for integrating SCS in the clinical care arena for improved patient care.
CD56bright NK IL-7Rα expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections
Authors: Judge Chelsey J , Kostadinova Lenche , Sherman Kenneth E , Butt Adeel A , Falck-Ytter Yngve , Funderburg Nicholas T , Landay Alan L , Lederman Michael M , Sieg Scott F , Sandberg Johan K , Anthony Donald D 
Published: April 2017

Abstract
Several lines of evidence support the concept that NK cells play an important role in control of hepatitis C virus (HCV) infection via cytokine secretion and cytotoxicity. IL-7 is a homeostatic cytokine with a role in T cell development, activation, proliferation, and cytokine secretion. The IL-7Rα chain [cluster of differentiation (CD)127] is expressed on NK cells, with greatest abundance on the CD56brightCD16dim/− (CD56bright) subset. Here, we measured CD127 expression on CD56bright, CD56dimCD16+ (CD56dim), or CD56negCD16+ (CD56neg) NK cell subsets of 25 uninfected donors (UD); 34 chronic HCV-infected, treatment-naiüve; 25 HIV-infected, virally suppressed on antiretroviral therapy (ART); and 42 HCV–HIV-coinfected subjects on ART. Interestingly, CD127 expression on CD56bright NK cells negatively correlated with HCV plasma levels in HCV monoinfection and HCV–HIV coinfection. IL-7 induced CD69 expression, as well as IFN-γ production, in CD56bright NK cells and also enhanced the IFN-α-induced CD69 expression on these cells. The latter was impaired in HIV infection. Furthermore, IL-7 induced B cell lymphoma 2 (BCL-2) expression and cell cycling of CD56bright NK cells, and this effect was impaired in HCV- and HIV-infected subjects. Whereas IL-7-stimulated CD56bright NK cell degranulation appeared intact in all cohorts, we observed impaired IL-7-activated NK cell cytolytic function in HCV- and HIV-infected subjects. Finally, IL-7-induced phosphorylation of STAT-5 (pSTAT-5) signaling was impaired in NK cells of subjects with chronic viral infection, and this was reversible upon 6 mo of viral suppression with IFN-free HCV therapy. These results implicate that IL-7-dependent NK cell activation and effector function may be other host immune surveillance mechanisms that are impaired in viral infections.
Percepção da influência das políticas e práticas de recursos humanos na satisfação com o trabalho
Authors: Paiva Luis Eduardo Brandão , Lima Tereza Cristina Batista de , Oliveira Thiago Sousa de , Pitombeira Suzete Suzana Rocha 
Published: April 2017

Esta pesquisa tem como objetivo analisar a influência das políticas e práticas de recursos humanos sobre a satisfação no trabalho, utilizando o mercado de trabalho no Ceará. O tema tem relevância devido à identificação da satisfação no trabalho como fator que causa mudanças nas atitudes e saúde dos funcionários afetando, portanto, a sua produtividade. Para tanto, foram traçados os seguintes objetivos específicos: identificar as dimensões com maior e menor relevância entre as políticas e práticas de RH; identificar quais dimensões apresentam maior e menor relevância na satisfação no trabalho; e analisar a relação entre satisfação no trabalho e a percepção das políticas e práticas de RH. Em relação aos procedimentos metodológicos, esta pesquisa é de natureza quantitativa, descritiva, com o método de coleta de dados survey, junto aos funcionários de empresas de grande porte que têm sede no Estado do Ceará. Os resultados obtidos apontam que os funcionários estão indiferentes em relação a sua satisfação com o trabalho, como também se sentem indiferentes quanto às políticas e práticas de recursos humanos. Já a correlação dos dados indicou que existe uma correlação moderada entre o fator remuneração e recompensas e a dimensão satisfação com o salário. Palavras-chave: Percepção. Políticas e Práticas de Recursos Humanos. Satisfação no Trabalho.
Kaiser Permanente Oakland Medical Center Optimizes Operating Room Block Schedule for New Hospital
Authors: Benchoff Brittney , Yano Candace Arai , Newman Alexandra 
Published: April 2017

In July 2014, Kaiser Permanente, a major integrated healthcare delivery consortium, opened a new hospital, replacing an existing hospital, adjacent to its headquarters in Oakland, California. Hospital staff needed to devise a new operating room schedule. In developing the schedule, the key decisions the staff would have to make were the type of block (i.e., a combination of surgery types that can be performed in the same operating room on the same day) to assign to each operating room on each day of the planning horizon. We report on the development and implementation of an integer programming model to generate a near-optimal block schedule. The approach differs from many in the literature because it considers both direct nursing costs and patient-related costs, and can accommodate a variety of practical constraints. Kaiser Permanente Oakland implemented the proposed schedule and continues to use it with minor modifications in response to subsequent growth and changes in patient demand patterns. Three major benefits of the schedule are that it: (1) satisfies almost all of the monthly block requirements in only four weeks, thereby releasing capacity to reduce the surgical backlog; (2) eliminates days with excess admissions, which would have required additional nursing staff; and (3) reduces the number of surgeries canceled due to an insufficient number of available beds.
Does Social Interaction Improve Learning Outcomes? Evidence from Field Experiments on Massive Open Online Courses
Authors: Zhang Dennis J. , Allon Gad , Van Mieghem Jan A. 
Published: April 2017

This paper studies how service providers can design social interaction among participants and quantify the causal impact of that interaction on service quality. We focus on education and analyze whether encouraging social interaction among students improves learning outcomes in massive open online courses (MOOCs), which are a new service delivery channel with universal access at reduced, if not zero, cost. We analyze three randomized experiments in a MOOC with more than 30,317 students from 183 countries. Two experiments study large-group interaction by encouraging a random subset of students to visit the course discussion board. The majority of students treated in these experiments had higher social engagement, higher quiz completion rates, and higher course grades. Using these treatments as instrumental variables, we estimate that one additional board visit causally increases the probability that a student finishes the quiz in the subsequent week by up to 4.3%. The third experiment studies small-group interaction by encouraging a random subset of students to conduct one-on-one synchronous discussions. Students who followed through and actually conducted pairwise discussions increased their quiz completion rates and quiz scores by 10% in the subsequent week. Combining results from these three experiments, we provide recommendations for designing social interaction mechanisms to improve service quality.
Alttestamentliche Prophetie als Thema religiöser und interreligiöser Bildung
Authors: Schlag Thomas 
Published: April 2017

Abstract
Compared to the other theological disciplines, the most intense and most innovative attempts at combining Old Testament tradition with a theological transfer to modernity are found in the area of religious pedagogy. Especially the treatment of prophetic literature in religious education has had a colorful past, which sharpens the eye for an up-to-date and appropriate presentation. The special challenge is to connect the traditional prophetic speech of God’s determining reality to the politically relevant power of orientation for the students’ lives in a theologically correct manner so that the truth claim of the biblical tradition can come to light freely.
Nanotechnology in food safety and quality assessment: potentiality of nanoparticles in diagnosis of foodborne pathogens
Authors: Primec Maša 
Published: April 2017

Abstract A rapid microbial detection in different biological and environmental material is a key of preventing several foodborne diseases. By implementing nanotechnology into food safety sector, a great step towards successful, reliable and sensible detection methods of foodborne pathogens has been achieved. Therefore, the aim of this review was to illustrate some of the principal functions of nanotechnology-based techniques, used for microbial detection in the last few years. Regarding consumer’s health, the review also discusses the question of safety, concerning human exposure to nanomaterials (NMs). Due to their different composition-unique properties, such as greater penetrability, reactivity and high surface to volume ratio, NMs have been coupled to several biomolecules and integrated in special system devices, resulting in improvement of sensitivity in transmitting biological signal informations in a shorter time. Among all the NMs, gold, magnetic and fluorescent nanoparticles (NPs) have been widely used, also in microbial diagnosis. Despite the success of linking nanotechnology to detection of foodborne pathogens, the exposure to various NMs could also be a matter of potential risk to human health, although conclusions still need to be definitely proven.
Identification of hypothetical duplicate accessions of plums (Prunus domestica L.) within the slovene plant gene bank collection using molecular markers
Authors: Šiško Metka 
Published: April 2017

Abstract The main goal of the Slovene Plant Gene Bank is preservation, maintenance and evaluation of traditional cultivars and other useful genotypes. The Faculty of Agriculture and Life Sciences houses among other plant materials also numerous accessions of plums (Prunus domestica L.). Duplicates among 15 accessions were studied using six microsatellite primer pairs. These microsatellite markers revealed an average of 7.67 alleles per locus, and a range of 4 to 10 different alleles per locus. The genetic distances between studied accessions were calculated using the Dice coefficient to form a dendrogram. The six SSRs were found to be adequate for differentiating among genotypes within the collection. Among the analysed accessions no duplicates were found.
Assessment of cassava supply response in Nigeria using vector error correction model (VECM)
Authors: Obayelu Oluwakemi Adeola , Ebute Samuel 
Published: April 2017

AbstractThe response of agricultural commodities to changes in price is an important factor in the success of any reform programme in agricultural sector of Nigeria. The producers of traditional agricultural commodities, such as cassava, face the world market directly. Consequently, the producer price of cassava has become unstable, which is a disincentive for both its production and trade. This study investigated cassava supply response to changes in price. Data collected from FAOSTAT from 1966 to 2010 were analysed using Vector Error Correction Model (VECM) approach. The results of the VECM for the estimation of short run adjustment of the variables toward their long run relationship showed a linear deterministic trend in the data and that Area cultivated and own prices jointly explained 74% and 63% of the variation in the Nigeria cassava output in the short run and long-run respectively. Cassava prices (P<0.001) and land cultivated (P<0.1) had positive influence on cassava supply in the short-run. The short-run price elasticity was 0.38 indicating that price policies were effective in the short-run promotion of cassava production in Nigeria. However, in the long-run elasticity cassava was not responsive to price incentives significantly. This suggests that price policies are not effective in the long-run promotion of cassava production in the country owing to instability in governance and government policies.
Chip bonding of low-melting eutectic alloys by transmitted laser radiation
Authors: Hoff Christian , Venkatesh Arjun , Schneider Friedrich , Hermsdorf Jörg , Bengsch Sebastian , Wurz Marc C. , Kaierle Stefan , Overmeyer Ludger 
Published: April 2017

Abstract
Present-day thermode bond systems for the assembly of radio-frequency identification (RFID) chips are mechanically inflexible, difficult to control, and will not meet future manufacturing challenges sufficiently. Chip bonding, one of the key processes in the production of integrated circuits (ICs), has a high potential for optimization with respect to process duration and process flexibility. For this purpose, the technologies used, so far, are supposed to be replaced by a transmission laser-bonding process using low-melting eutectic alloys. In this study, successful bonding investigations of mock silicon chips and of RFID chips on flexible polymer substrates are presented using the low-melting eutectic alloy, 52In48Sn, and a laser with a wavelength of 2 μm.
The effects of 20-m repeated sprint training on aerobic capacity in college volleyball players
Authors: Kaynak Kerimhan , Eryılmaz Selcen Korkmaz , Aydoğan Sami , Mihailov Dimitar 
Published: April 2017

Summary
Study aim: The purpose of this study was to examine the effects of a repeated sprint training program in addition to volleyball training on the aerobic capacity of college volleyball players.
Materials and methods: Eighteen male volleyball players were randomly assigned to either an experimental group (n = 9, age: 21.2 ± 1.3 years) or a control (n = 9, age: 21.2 ± 1.6 years) group. Both groups followed a traditional volleyball training program three times per week for 6 weeks. The experimental group additionally performed a repeated sprint training protocol immediately before each volleyball training session. The repeated sprint training consisted of 1-3 sets of 5 × 20 m maximal sprints with 20 seconds of active recovery between sprints and 4 min of passive recovery between sets. Before and after the 6-week training period, all participants performed an incremental treadmill test to determine maximal oxygen uptake (VO2max) and time to exhaustion, and the repeated sprint test (10 × 20 m with a 20‑second recovery between each sprint).
Results: The experimental group showed significant improvements in VO2max (+7.1 ± 4.8%; p = 0.001) and running time to exhaustion (+15.8 ± 6.8%; p = 0.004) after training. The best 20-m sprint time (−2.3 ± 2.5%; p = 0.029), mean sprint time (−5.3 ± 3.1%; p = 0.001) and fatigue index (−34.1 ± 28.2%; p = 0.012) also improved significantly in the experimental group. None of these variables changed significantly in the control group (p > 0.05).
Conclusions: The current findings indicate that the addition of a repeated sprint training program can improve both the aerobic capacity and anaerobic performance of college volleyball players.
Swailing affects seed germination of plants of European bio-and agricenosis in a different way
Authors: Bączek-Kwinta Renata 
Published: April 2017

AbstractSwailing as a part of agricultural practice is an illegal habit in many European countries. The indirect effect of swailing is the emission of volatiles (SGV), hence the aim of the study was to identify their impact to seeds of different species occurring or grown Europe. It was carried out on seeds of 29 species of 10 botanical families within the angiosperms. The response to SGV was more or less differentiated within a family, and even within the species, e.g. in the case of tomato. The stimulation of germination and/or increased seedling vigour was established in celery, green- and red-leafed basil, white and red cabbage, white clover and wild thyme. The same effect was noticed for the seeds of stratified broadleaf plantain and the positively photoblastic seeds of German chamomile germinated in darkness. The inhibition of seed germination and/ or reduced seedling vigour was demonstrated in case of caraway, dill and forget-me-not. Similar results were obtained in the experiments carried outin vitroand in the soil, hence it can be assumed that the indirect impact of SGV on plant habitat composition is likely. The interaction of SGV compounds with seed testa and seed phytohormones is discussed.
Chemical composition and antifungal potential of medicinal plants against seedborne mycoflora of eggplant (Solanum melongena L.)
Authors: Ashiq Bushra , Chohan Sobia , Perveen Rashida , Abid Muhammad , Mehmood Mirza Abid 
Published: April 2017

AbstractAntifungal activities of medicinal plants were observed against seedborne mycoflora of eggplant (Solanum melongena). The effect of ethanolic leaf extracts of Mangifera indica, Mentha spicata, Citrus limon, Eucalyptus camaldulensis against four isolated fungal species including Fusarium oxysporum, Aspergillus flavus, Rhizopus stolonifer and Penicillium digitatum was evaluated at various concentrations, by using the poisoned food technique. The impact of the extracts on seed germination and growth of eggplant was assessed by seed treatment and growth in a greenhouse experiment. Total flavonoids of E. camaldulensis were analyzed through spectrophotometer, using quercetin as a standard. Physico-chemical parameters were also determined. Antifungal activity showed that maximum inhibition percentage of P. digitatum (67.78%) and F. oxysporum (64.44%) was observed at the highest concentration (80%) of C. limon and E. camaldulensis extracts, respectively, followed by M. spicata extract against A. fl avus (63.33%) and R. stolonifer (52.22%). Least inhibition percentage of F. oxysporum, P. digitatum, R. stolonifer and A. flavus was 6.67, 7.78, 14.44 and 16.67%, respectively, at the lowest (20%) concentration of M. spicata. The greenhouse experiment showed variations in seedling germination and post-germination growth. E. camaldulensis extract showed an increase in percent germination (78.98%) over untreated control (62.83%), root and shoot length and fresh and dry weight of root and shoot with the consequent reduction in disease symptoms. Phytochemical analysis depicted the presence of alkaloids, flavonoids, tannins, saponins in all extracts while steroids and glycosides were absent. A fair amount (10.38 mg QE g-1DF) of flavonoid was present in leaf extract of E. camaldulensis. Physico-chemical analysis showed pH of 4.6, ash content of 0.41% and weight loss on drying of 8.14%.
Judicial Activism Contributing to the Understanding of Social State Principle(S) – Constitutional Court of Slovenia at the Crossroads
Authors: Brezovar Nejc 
Published: April 2017

Abstract
The abstract nature of Constitutional principles, such as the social state principle, requires further interpretation to determine their concrete substance. Their realization is primarily the duty of politics and the legislator. Yet the Constitutional Courts can substantially contribute to developing the contents and nature of social state principles. This paper attempts to show, through examples from Slovenian judicature, how Constitutional Courts can, with the use of (limited) judicial activism, form and shape social politics and their main principles. The Slovenian Constitutional Court is usually relatively restrained in its interpretations of the social state principles respecting the primary authority of the legislator in regulating the area. On the other hand, we cannot ignore the important role the Constitutional Court and its judicature play in developing the substance of social state principles. This is usually done by means of dynamic and evolutionary interpretations. After analysing the judicature,we can conclude that some of the interconnected social state principals developed by the Court, which play a vital role in understanding the essence of a social state and social rights it provides, are: social balance, social security, social justice, solidarity, a minimum protection of existence, prevention of social exclusion, proving the important contribution of Constitutional Courts to the understanding of the concept of social state principals.
Effectiveness of Marketing Mix Activities in Engineering Companies in the Czech Republic
Authors: Milichovský František 
Published: April 2017

Abstract
The effectiveness of marketing activities has become important, especially in the industrial environment. In an industrial environment, there is a prerequisite for the success of strategic marketing objectives in relation to corporate strategy. The main aim of the paper is to determine whether the realization of marketing activities is influenced by corporate size. For this reason, a questionnaire survey was used, focusing on engineering companies operating in the Czech Republic. To process the results of the questionnaire survey, both basic types of descriptive statistics and Pearson’s chi-square test were used on the selected dataset. The data were processed using the statistical program IBM SPSS Statistics 22. The conclusions provide characteristics of the limitations of research and its potential further direction.
European Institutions On Social Media ‒ Shaping the Notion of European Citizenship
Authors: Gaušis Eduards 
Published: April 2017

Abstract
This study supports topical discussion about the possibilities to use social media as a tool for citizen involvement in democratic processes. Contemporary academic views and statistics on the use of social media for communication between institutions and citizens are gathered in the study. The research focus is on social media publications, analysing the content of four European Parliament’s social media accounts in Latvian during the period from 1 July 2015 to 31 December 2015. The aim of the research is to identify the potential of the information published on European Parliament’s social media accounts in shaping the European citizenship notion among youth in Latvia. The conclusions present clear idea on the frequency of European citizenship topics in the corresponding social media accounts as well as on the amount of feedback and the share of youth involvement in this communication. The results of the study suggest that for now European Parliament’s social media publications in Latvian have little potential to develop the notion of European citizenship and there is a need for a well-considered use of social media to assure that the opportunity for two-way communication is fruitfully used.