bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain state and polarity dependent modulation of brain networks by transcranial direct current stimulation
Authors: Li Lucia M. , Violante Ines R. , Leech Rob , Ross Ewan , Hampshire Adam , Opitz Alexander , Rothwell John C. , Carmichael David W. , Sharp David J. 
Published: August 2017

AbstractTranscranial direct current stimulation (TDCS) has been widely used to improve cognitive function. However, current deficiencies in mechanistic understanding hinders wider applicability. To clarify its physiological effects, we acquired fMRI whilst simultaneously acquiring TDCS to the right inferior frontal gyrus (rIFG) of healthy human participants, a region involved in coordinating activity within brain networks. TDCS caused widespread modulation of network activity depending on brain state (‘rest’ or choice reaction time task) and polarity (anodal or cathodal). During task, TDCS increased salience network activation and default mode network deactivation, but had the opposite effect during ‘rest’. Furthermore, there was an interaction between brain state and TDCS polarity, with cathodal effects more pronounced during task performance and anodal effects more pronounced during ‘rest’. Overall, we show that rIFG TDCS produces brain state and polarity dependent effects within large-scale cognitive networks, in a manner that goes beyond predictions from the current literature.
Role for Ribosome-Associated Complex and Stress-Seventy subfamily B (RAC-Ssb) in integral membrane protein translation
Authors: Acosta-Sampson Ligia , Döring Kristina , Lin Yuping , Yu Vivian Y. , Bukau Bernd , Kramer Günter , Cate Jamie H. D. 
Published: August 2017

ABSTRACTTargeting of most integral membrane proteins to the endoplasmic reticulum is controlled by the signal recognition particle (SRP), which recognizes a hydrophobic signal sequence near the protein N-terminus. Proper folding of these proteins is monitored by the unfolded protein response, and involves protein degradation pathways to ensure quality control. Here, we identify a new pathway for quality control of major facilitator superfamily transporters that occurs before the first transmembrane helix–the signal sequence recognized by SRP–is made by the ribosome. Increased rates of translation elongation of the N-terminal sequence of these integral membrane proteins can divert the nascent protein chains to the ribosome-associated complex (RAC) and Stress-Seventy Subfamily B (Ssb) chaperones. We also show that quality control of integral membrane proteins by RAC-Ssb couples translation rate to the unfolded protein response, which has implications for understanding mechanisms underlying human disease and protein production in biotechnology.
Identification of Iridoid Synthases fromNepetaspecies: Iridoid cyclization does not determine nepetalactone stereochemistry
Authors: Sherden Nathaniel H. , Lichman Benjamin , Caputi Lorenzo , Zhao Dongyan , Kamileen Mohamed O. , Robin Buell C. , O’Connor Sarah E. 
Published: August 2017

Graphical AbstractIridoid synthase fromNepeta cateria(catnip) andNepeta mussinii, have been cloned and characterized.AbstractNepetalactones are iridoid monoterpenes with a broad range of biological activities produced by plants in theNepetagenus. However, none of the genes for nepetalactone biosynthesis have been discovered. Here we report the transcriptomes of twoNepetaspecies, each with distinctive profiles of nepetalactone stereoisomers. As a starting point for investigation of nepetalactone biosynthesis inNepeta, these transcriptomes were used to identify candidate genes for iridoid synthase homologs, an enzyme that has been shown to form the core iridoid skeleton in several iridoid producing plant species. Iridoid synthase homologs identified from the transcriptomes were cloned, heterologously expressed, and then assayed with the 8-oxogeranial substrate. These experiments revealed that catalytically active iridoid synthase enzymes are present inNepeta,though there are unusual mutations in key active site residues. Nevertheless, these enzymes exhibit similar catalytic activity and product profile compared to previously reported iridoid synthases from other plants. Notably, four nepetalactone stereoisomers with differing stereochemistry at the 4αand 7αpositions – which are generated during the iridoid synthase reaction – are observed at different ratios in variousNepetaspecies. This work strongly suggests that the variable stereochemistry at these 4αand 7αpositions of nepetalactone diastereomers is established further downstream in the iridoid pathway inNepeta. Overall, this work provides a gateway into the biosynthesis of nepetalactones inNepeta.HighlightsSpecies within theNepetagenus (such as catnip) produce nepetalactone iridoidsThe enzymes that produce the iridoid scaffold of nepetalactone were identified from two species ofNepetaThe iridoid synthase enzymes are not responsible for the stereochemical variation in these iridoids
Helpers increase daily survival rate of Southern Lapwing (Vanellus chilensis) nests during the incubation stage
Authors: Santos Eduardo S. A. , Macedo Regina H. 
Published: August 2017

AbstractCooperative breeding is characterized by reproduction in the presence of helpers. What impact these helpers have on the reproductive success of group members is one of the long-standing questions in the cooperative breeding literature. In cooperative species, helpers are known to provide benefits during multiple stages or at a particular stage of the reproductive cycle. The aim of this study was to investigate whether helpers increased the daily survival rate of nests during the incubation stage in the Southern Lapwing (Vanellus chilensis), a crested plover with a cooperative breeding system. Southern Lapwings have a variable mating system, with some breeding groups composed of unassisted pairs, and others that breed in the presence of helpers. Our best supported model indicated a positive effect of the presence of helpers on the daily survival rate of nests, leading to a probability of nest success (i.e., survival until hatching) of 83%, compared to 51% for nests of unassisted pairs. But a null model had a similar model weight as the best supported model and was the second-best model. Our study provides evidence that helpers influence egg survival during the egg-incubation stage, which could influence the fitness of breeders.
Low-level mitochondrial heteroplasmy modulates DNA replication, glucose metabolism and lifespan in mice
Authors: Hirose Misa , Schilf Paul , Gupta Yask , Zarse Kim , Künstner Axel , Busch Hauke , Yin Junping , Wright Marvin N , Ziegler Andreas , Vallier Marie , Belheouane Meriem , Baines John F , Tautz Diethard , Johann Kornelia , Oelkrug Rebecca , Mittag Jens , Lehnert Hendrik , Othman Alaa , Jöhren Olaf , Schwaninger Markus , Prehn Cornelia , Adamski Jerzy , Shima Kensuke , Rupp Jan , Haesler Robert , Fuellen Georg , Köhling Rüdiger , Ristow Michael , Ibrahim Saleh M 
Published: August 2017

SummaryMutations in mitochondrial DNA (mtDNA) lead to heteroplasmy, i.e. the intracellular coexistence of wild-type and mutant mtDNA strands, which impact a wide spectrum of diseases but also physiological processes, including endurance exercise performance in athletes. However, the phenotypic consequences of limited levels of naturally-arising heteroplasmy have not been experimentally studied to date. We hence generated a conplastic mouse strain carrying the mitochondrial genome of a AKR/J mouse strain (B6-mtAKR) together with a C57BL/6J nuclear genomic background, leading to >20% heteroplasmy in the origin of light-strand DNA replication (OriL). These conplastic mice demonstrate a shorter lifespan as well as dysregulation of multiple metabolic pathways, culminating in impaired glucose metabolism, compared to wild-type C57BL/6J mice carrying lower levels of heteroplasmy. Our results indicate that physiologically relevant differences in mtDNA heteroplasmy levels at a single, functionally important site impair metabolic health and lifespan in mice.HighlightsWe identify heteroplasmy of the adenine-repeat variation (9 to 13A) in nt5172 in the origin of light-strand DNA replication (OriL) in inbred mice.B6-mtAKRmice carry >20% 12A heteroplasmy in the OriL, while B6 mice carry only ∼ 10% heteroplasmy.The level of 12A heteroplasmy correlates to mtDNA copy number, glucose metabolism, and lifespan in mice.Given the established role of mtDNA heteroplasmy in regards to endurance exercise performance in athletes, these findings may impact our understanding of metabolism and aging in humans.
Tadpole-like conformations of huntingtin exon 1 with expanded polyglutamine engenders novel interactions in cells
Authors: Newcombe Estella A. , Ruff Kiersten M. , Sethi Ashish , Ormsby Angelique R. , Ramdzan Yasmin M. , Fox Archa , Purcell Anthony W. , Gooley Paul R. , Pappu Rohit V. , Hatters Danny M. 
Published: August 2017

ABSTRACTSoluble huntingtin exon 1 (Httex1) with expanded polyglutamine (polyQ) engenders neurotoxicity in Huntington’s disease. To uncover the physical basis of this toxicity, we performed structural studies of soluble Httex1 for wild type and mutant polyQ lengths. Nuclear magnetic resonance experiments show evidence for conformational rigidity across the polyQ region. In contrast, hydrogen-deuterium exchange shows absence of backbone amide protection, suggesting negligible persistence of hydrogen bonds. The seemingly conflicting results are explained by all-atom simulations, which show that Httex1 adoptstadpole-likestructures with a globular head encompassing the N-terminal amphipathic and polyQ regions and the tail encompassing the C-terminal proline-rich region. The surface area of the globular domain increases monotonically with polyQ length. This stimulates sharp increases in gain-of-function interactions in cells for expanded polyQ, and one of these interactions is with the stress-granule protein Fus. Our results highlight plausible connections between Httex1 structure and routes to neurotoxicity.
Lead-lag relationship between futures and spot FX market in India
Authors: Kharbanda Varuna , Singh Archana 
Published: August 2017

PurposeThe purpose of this paper is to study the lead-lag relationship between the futures and spot foreign exchange (FX) market in India to understand the price discovery mechanism and the relationship between these two markets.Design/methodology/approachThe estimation of lead-lag relationship is realized in three steps. First unit root and stationarity tests (Augmented Dickey-Fuller, Phillips-Perron, and Kwiatkowski-Phillips-Schmidt-Shin) are applied to check the stationarity of the data. Second, cointegration tests (Engle and Granger’s residual based approach and Johansen’s cointegration test) are applied to determine long run relationship between the markets. Third, error correction estimation is carried out by applying Vector Error Correction Model (VECM) to determine the leading market.FindingsThe study finds that there is a long run relationship between the futures and spot market where the futures market has emerged as the leading market for the four currencies studied in the paper.Originality/valueMajorly, the studies on Indian FX market limit themselves to identifying the efficiency of the market and the studies which talk about the lead-lag relationship focus on the Indian stock market. This paper enhances the existing literature on Indian FX market by exploring the less explored subject of the lead-lag relationship between futures and spot FX market in India.
The interrelationship between net interest margin and non-interest income: evidence from Vietnam
Authors: Le Tu DQ 
Published: August 2017

Purpose
The purpose of this paper is to investigate the interrelationship between non-interest income (NII) and net interest margin (NIM) in the Vietnamese banking system between 2006 and 2015. Thereafter, the impact of NII on risk-adjusted returns is also examined.

Design/methodology/approach
Various analysis techniques are used to achieve the research objectives.

Findings
The findings show a negative two-way link between NII and NIM, thus supporting the subsidisation hypothesis. Furthermore, NII is found to have a negative impact on risk-adjusted returns. When observing this relationship in sub-samples, the findings indicate that the negative impact of NII on risk-adjusted returns still holds in the first subsample (2006-2011). The coefficient of NII becomes positive but not significant for the subsequent period (2012-2015). In addition, the Spearman rank-order correlations of returns on assets and NII for both sub-samples are negative. Together, the author concludes that there are no diversification benefits in the Vietnamese banking system.

Practical implications
The evidence suggests a trade-off between non-interest activities and traditional lending ones. In addition, the findings demonstrate that the Vietnamese banks may use NIIs to expand leverage and herd by coordinating NII strategy during the economic downturns. Thus, the banking system may be exposed to a greater risk. The research has implications for bank supervisors, policy-makers and bank managers.

Originality/value
This study is the first attempt to investigate the interrelationships between net NII and NIM in the Vietnamese banking system.
Students’ satisfaction with hostel accommodations in higher education institutions
Authors: Oke Ayodeji Emmanuel , Aigbavboa Clinton Ohis , Raphiri Marcia M. 
Published: August 2017

Purpose
There are several types of accommodation available to students of higher education institutions, and it is the responsibility of the institutions to focus on factors that can attract and retain students to their residences. This study aims to investigate satisfaction of higher institutions students with on-campus accommodation with an emphasis on the ethnicity of the students.

Design/methodology/approach
Using convenience sampling method, questionnaires were administered to students of selected residences in the study area. Mean item score was used to rank identified factors, whereas mean gap and Kruskal–Wallis K-test were adopted to examine the difference in opinion of students from various ethnic groups.

Findings
Overall, major features that make students dissatisfied with university-owned accommodations are enforcement of rule that compels all students to move out with their belongings during each recess, the effectiveness of the lift system, the size of wardrobe and closet, laundry service in the residence, numbers of electrical sockets and window quality. Considering dwelling unit features, neighbourhood, environmental, building quality and services provided by residence management, this study reveals that there is a significant difference in the satisfaction of students of diverse ethnic groups with their accommodation.

Research limitations/implications
The study was limited to on-campus residences owned by higher education institutions in South Africa. However, the findings of the study can be adopted for off-campus residences, and such owned by private individuals, agencies and other bodies provided are approved by the institutions.

Originality/value
The findings of this study will help management of higher education institutions to improve quality of services in their residences for the satisfaction of their students. Developers, contractors and other stakeholders involved in construction of these residences will also find the findings useful in designing and construction of the facilities.
Rethinking the challenges to the pricing of projects in the Ghanaian construction industry
Authors: Kissi Ernest , Ahadzie Divine Kwaku , Adjei-Kumi Theophilus , Badu Edward 
Published: August 2017

Purpose
The aim of the paper is to examine the challenges to the pricing of construction projects in the Ghanaian industry. This remains a persistent problem among construction stakeholders because price is the basis for which offers are made. Project stakeholders are always faced with challenges relating to effective and efficient pricing system making it difficult to achieve value for money.

Design/methodology/approach
Research data were collected through a questionnaire survey involving quantity surveyors in the Ghanaian construction industry. Data collected were analyzed using exploratory factor analysis which helped to reduce the factors into six components. Confirmatory factor analysis (CFA) was further carried out to explore whether these six observed variables would form a latent variable called challenges to pricing of construction projects.

Findings
The paper highlighted six underlying challenges which were cost-related, managerial-related, quality-related, variation of works, poor understanding of the terms of contract and time-related. Strong and significant relationships were found between all six of the observed variables. A CFA confirmed that they formed a latent variable the authors called challenges to pricing of construction projects.

Research limitations/implications
The findings clearly can serve as a reference for both developed and developing countries in the quest for finding an antidote to pricing challenges. In addition, use of CFA improves measurement of the constructs and thus enhances understanding of the underlying components of a construct and their individual relationship with pricing. The paper is particularly useful for policy making and industry regulation because it identifies some of the factors that need attention.

Originality/value
This paper presents an exceptional contribution to the construction management literature by concentrating on the factors that challenge effective and efficient pricing system in the construction industry.
Mechanisms of subzero growth in the cryophile Planococcus halocryophilus determined through proteomic analysis
Authors: Raymond‐Bouchard Isabelle , Chourey Karuna , Altshuler Ianina , Iyer Ramsunder , Hettich Robert L. , Whyte Lyle G. 
Published: August 2017

SummaryThe eurypsychrophilic bacterium Planococcus halocryophilus is capable of growth down to −15°C, making it ideal for studying adaptations to subzero growth. To increase our understanding of the mechanisms and pathways important for subzero growth, we performed proteomics on P. halocryophilus grown at 23°C, 23°C with 12% w/v NaCl and −10°C with 12% w/v NaCl. Many proteins with increased abundances at −10°C versus 23°C also increased at 23C‐salt versus 23°C, indicating a closely tied relationship between salt and cold stress adaptation. Processes which displayed the largest changes in protein abundance were peptidoglycan and fatty acid (FA) synthesis, translation processes, methylglyoxal metabolism, DNA repair and recombination, and protein and nucleotide turnover. We identified intriguing targets for further research at −10°C, including PlsX and KASII (FA metabolism), DD‐transpeptidase and MurB (peptidoglycan synthesis), glyoxalase family proteins (reactive electrophile response) and ribosome modifying enzymes (translation turnover). PemK/MazF may have a crucial role in translational reprogramming under cold conditions. At −10°C P. halocryophilus induces stress responses, uses resources efficiently, and carefully controls its growth and metabolism to maximize subzero survival. The present study identifies several mechanisms involved in subzero growth and enhances our understanding of cold adaptation.
How to lose your clinical balance, while holding on to your therapeutic bearings, and other ideas on working therapeutically with a ‘treatment resistant’ client diagnosed with Borderline Personality Disorder
Authors: Boardman Don 
Published: August 2017

AbstractThis article describes a clinical case where an Advanced Brief Strategic Therapy Model (Gibson and Boardman, ; Gibson et al., ; Nardone and Watzlawick, ) was used with a woman presenting with presumed Borderline Personality Disorder (BPD). In the world of therapy, the process of change can often be hard to predict at the best of times and extremely unpredictable during the worst. It can be difficult for the curious therapist to know how and what to look for as indicators of change when the larger picture may appear to remain unchanged in a client's life. I am curious about those therapy cases which present with great difficulties attached to them and which often drain much of our energy, vim and vigour as psychotherapists with often little apparent return on the investment we make. That is, if we measure that return in terms of reported change by the client, as opposed to privileging therapeutic disposition.Practitioner points

This article explores how an Advanced Brief Strategic Therapy Model was used with a client diagnosed with Borderline Personality Disorder
I critique the often misunderstood idea of embodied cybernetics as an interpretation of the therapeutic process
Drawing on clinical practice, I describe some of the challenges in working with a client who often dispensed with rationality and employed non‐ordinary logic in her modus operandi
Managerial Career Choices: Evidence from South Australian Local Government
Authors: Jorgensen Bradley S. , Martin John F. , Nursey‐Bray Melissa 
Published: August 2017

AbstractThere are structural and individual factors that contribute to and compound the current and continuing under‐representation of women in leadership and senior management positions. We explore these factors by investigating the beliefs and intentions of male and female senior managers with respect to applying for promotion to executive level in local government organisations in South Australia. Survey data from 148 senior managers indicated that men and women have similar belief structures when it comes to their intentions to apply for promotion in South Australian local government. The imbalance in the proportion of women and men in CEO positions in South Australian local government, we suggest, reflects earlier findings of the inherent bias towards men in the selection process for these positions. Our analysis supports a number of structural and managerial recommendations, which we believe will address this imbalance overtime.
Current Immunoassay Methods for the Rapid Detection of Aflatoxin in Milk and Dairy Products
Authors: Matabaro Emmanuel , Ishimwe Nestor , Uwimbabazi Eric , Lee Byong H. 
Published: August 2017

AbstractThe presence of mycotoxins in foodstuff causes serious health problems to consumers and economically affects the food industry. Among the mycotoxins, aflatoxins are very toxic and highly carcinogenic contaminants which affect the safety of many foods, and therefore endanger human health. Aflatoxin M1(AFM1) found in milk results from the biotransformation of aflatoxin B1. Many efforts have been made to control the source of AFM1from farmers to dairy product companies. However, AFM1escapes ordinary methods of food treatment such as cooking, sterilization, and freezing, hence it appears in milk and dairy products. The presence of high levels of AFM1constitutes an alarming threat as milk and dairy products contain essential nutrients for human health, especially for infants and children. For this reason, there is a pressing need for developing a fast and reliable screening method for detecting trace aflatoxins in food. Several analytical methods based on high‐performance liquid chromatography (HPLC) and mass spectroscopy have been used for aflatoxin detection; however, they are expensive, time‐consuming, and require many skills. Recently, immunoassay methods, including enzyme‐linked immunosorbent assay (ELISA), immunosensors, and lateral flow immunoassay (LFIA), have been preferred for food analysis because of their improved qualities such as high sensitivity, simplicity, and capability of onsite monitoring. This paper reviews the new developments and applications of immunoassays for the rapid detection of AFM1in milk.