bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein p53 Binding to Cisplatin‐modified DNA Targets Evaluated by Modification‐specific Electrochemical Immunoprecipitation Assay
Authors: Tichy Vlastimil , Sebest Peter , Orsag Petr , Havran Ludek , Pivonkova Hana , Fojta Miroslav 
Published: September 2016

AbstractStudies of protein interactions with chemically modified nucleic acids are of importance in various areas of biomolecular and biomedical research, including investigations of the binding of proteins important in medicine with DNA modified with drugs and diagnostic applications of modified DNAs in biosensing and bioanalysis. Chemical modification of DNA substrates with various species inside or outside specific protein binding sites can affect the protein‐DNA recognition. In this paper we present a simple electrochemical immunoprecipitation technique designed for evaluation of the effects of antitumor drug cisplatin on the p53‐DNA binding. The cisplatin‐DNA adducts are utilized as electroactive labels allowing a facile determination of the p53‐bound modified DNA. Effects observed using this technique accord with results of previous biochemical assays. This approach is potentially applicable in studies that deal with the influence of any electroactive DNA modifications on the protein‐DNA binding.
Parameterizing the binding properties of dissolved organic matter with default values skews the prediction of copper solution speciation and ecotoxicity in soil
Authors: Djae Tanalou , Bravin Matthieu N. , Garnier Cédric , Doelsch Emmanuel 
Published: September 2016

Abstract

Parameterizing speciation models by setting the percentage of dissolved organic matter (DOM) that is reactive (% r-DOM) toward metal cations at a single 65% default value is very common in predictive ecotoxicology. The authors tested this practice by comparing the free copper activity (pCu2+ = –log10[Cu2+]) measured in 55 soil sample solutions with pCu2+ predicted with the Windermere humic aqueous model (WHAM) parameterized by default. Predictions of Cu toxicity to soil organisms based on measured or predicted pCu2+ were also compared. Default WHAM parameterization substantially skewed the prediction of measured pCu2+ by up to 2.7 pCu2+ units (root mean square residual = 0.75–1.3) and subsequently the prediction of Cu toxicity for microbial functions, invertebrates, and plants by up to 36%, 45%, and 59% (root mean square residuals ≤9 %, 11%, and 17%), respectively. Reparametrizing WHAM by optimizing the 2 DOM binding properties (i.e., % r-DOM and the Cu complexation constant) within a physically realistic value range much improved the prediction of measured pCu2+ (root mean square residual = 0.14–0.25). Accordingly, this WHAM parameterization successfully predicted Cu toxicity for microbial functions, invertebrates, and plants (root mean square residual ≤3.4%, 4.4%, and 5.8%, respectively). Thus, it is essential to account for the real heterogeneity in DOM binding properties for relatively accurate prediction of Cu speciation in soil solution and Cu toxic effects on soil organisms. Environ Toxicol Chem 2017;36:898–905. © 2016 SETAC
Managing false diffusion during second‐order upwind simulations of liquid micromixing
Authors: Bailey Robert T. 
Published: September 2016

SummaryLiquid mixing is an important component of many microfluidic concepts and devices, and computational fluid dynamics (CFD) is playing a key role in their development and optimization. Because liquid mass diffusivities can be quite small, CFD simulation of liquid micromixing can over predict the degree of mixing unless numerical (or false) diffusion is properly controlled. Unfortunately, the false diffusion behavior of higher‐order finite volume schemes, which are often used for such simulations, is not well understood, especially on unstructured meshes. To examine and quantify the amount of false diffusion associated with the often recommended and versatile second‐order upwind method, a series of numerical simulations was conducted using a standardized two‐dimensional test problem on both structured and unstructured meshes. This enabled quantification of an ‘effective’ false diffusion coefficient (Dfalse) for the method as a function of mesh spacing. Based on the results of these simulations, expressions were developed for estimating the spacing required to reduce Dfalse to some desired (low) level. These expressions, together with additional insights from the standardized test problem and findings from other researchers, were then incorporated into a procedure for managing false diffusion when simulating steady, liquid micromixing. To demonstrate its utility, the procedure was applied to simulate flow and mixing within a representative micromixer geometry using both unstructured (triangular) and structured meshes. Copyright © 2016 John Wiley & Sons, Ltd.
Simple and ecofriendly synthesis of dihydropyrimidinones (thiones), dihydropyridines, and pyridines using 3‐formylchromones as substrates assisted by a recyclable Preyssler heteropolyacid
Authors: Sanchez Laura M. , Pasquale Gustavo , Sathicq Ángel , Ruiz Diego , Jios Jorge , Ferreira de Souza Andrea L. , Romanelli Gustavo P. 
Published: September 2016

AbstractSeveral dihydropyrimidinones/thiones, 1,4‐dihydropyridines, and pyridine derivatives were prepared in very good yields and purity values. The corresponding reactions were carried out by employing a bulk Preyssler heteropolyacid H14[NaP5W29MoO110] as an efficient and recyclable catalyst. The preparation of pyridine derivatives was carried out not through a usual procedure, i.e., the opening of the γ‐pyrone ring of 3‐formylchromone. In general, reactions took place in solvent‐free conditions at 80°C during short reaction times.
Incidence of small lymph node metastases in patients with nasopharyngeal carcinoma: Clinical implications for prognosis and treatment
Authors: Xu Peng , Min Yanmei , Blanchard Pierre , Feng Mei , Zhang Peng , Luo Yangkun , Fan Zixuan , Lang Jinyi 
Published: September 2016

ABSTRACTBackgroundPatients with nasopharyngeal carcinoma (NPC) often present small lymph nodes. The purpose of this study was to determine the prognostic impact on local recurrence of small lymph nodes, defined as smaller than 10 mm in greatest diameter.MethodsConsecutive patients treated by intensity‐modulated radiotherapy (IMRT) for pathologically confirmed NPC were analyzed retrospectively. Those without small lymph nodes were excluded from the study. From January 2005 to January 2011, 275 patients with NPC represented with 2722 small lymph nodes, which were analyzed. Small lymph node axial diameter was measured using CT/MRI before radiotherapy (RT), at 50 Gy, and 6 months after the end of RT. The dose received by the small lymph nodes and the changes in lymph node diameter were recorded. Clinical endpoints were overall survival (OS), locoregional control, disease‐specific survival (DSS), and distant metastasis‐free survival. Median follow‐up time was 55 months (range, 5–96 months).ResultsPatients were grouped according to the dose received by the small lymph nodes and the number of small lymph nodes. Only 27 patients (9.8%) had a decrease ≥50% in the small lymph node diameter at 50 Gy and 53 patients (19.3%) had a decrease ≥50% at 6 months after RT. The 5‐year locoregional control, distant metastasis‐free survival, DSS, and OS were 93.5%, 85.2%, 88.8%, and 87.0%, respectively. Multivariate analyses showed that T classification and N classification are independent prognostic factors for OS. However, the dose received and the numbers of small lymph nodes were not statistically associated with any of the survival endpoints.ConclusionIn the IMRT era, N classification remains an independent prognostic factor in NPC. However, the incidence of small lymph nodes is not a significant prognostic factor in patients with NPC. The presence of small lymph nodes should not influence the nodal contouring or the dose delivered to nodal areas. © 2016 Wiley Periodicals, Inc. Head Neck 39: 305–310, 2017
Megastigmane Glycosides from Docynia indica and Their Anti‐inflammatory Activities
Authors: Dung Hoang Viet , Bach Nguyen Van , Trung Trinh Nam , Nhiem Nguyen Xuan , Tai Bui Huu , Kiem Phan Van , Park SeonJu , Lee Taek Hwan , Kim Sun Yeou , Kim Seung Hyun 
Published: September 2016

Using various chromatographic methods, three new megastigmane glycosides, docynicasides A – C (1 – 3) and ten known, (6S,9R)‐vomifoliol 9‐O‐β‐d‐xylopyranosyl‐(1′′→6′)‐O‐β‐d‐glucopyranoside (4), hyperin (5), quercitrin (6), quercetin 3‐α‐l‐arabinofuranoside (7), naringenin 7‐O‐β‐d‐glucopyranoside (8), phloridzin (9), phloretin 2′‐O‐β‐d‐xylopyranosyl‐(1→6)‐β‐d‐glucopyranoside (10), pinosylvin 3‐O‐β‐d‐glucopyranoside (11), tormentic acid (12), and chlorogenic acid methyl ester (13) were isolated from the fruits of Docynia indica. Their chemical structures were elucidated by physical and chemical methods. All the isolated compounds were evaluated for the inhibitory activity on NO production in LPS‐stimulated BV2 cells. As the results, compounds 3 – 5 showed significant inhibitory activity on LPS‐stimulated NO production in BV2 cells with the IC50 values ranging from 21.0 to 29.3 μm.
Aloeverasides A and B: Two Bioactive C‐Glucosyl Chromones from Aloe vera Resin
Authors: Rehman Najeeb Ur , Hussain Hidayat , Khiat Mohammed , Al‐Riyami Samia Ahmed , Csuk René , Khan Husain Yar , Abbas Ghulam , Al‐Thani Ghanim Salim , Green Ivan R. , Al‐Harrasi Ahmed 
Published: September 2016

Two new C‐glucosyl chromones named aloeverasides A (1) and B (2) were isolated from the resin of Aloe vera (L.) Burm.f. The structures of the two new natural products were elucidated based on 1D‐ (1H‐ and 13C‐NMR) and 2D‐NMR (COSY, HSQC, and HMBC) spectroscopic techniques and mass spectrometry (ESI‐MS). Aloeverasides A (1) and B (2) were evaluated for their anticancer activity, and both induced a 76.4 and 70.5% growth inhibition of the breast cancer cell line (MDA‐MB‐231) at a concentration of 100 μm. Both compounds were also evaluated for their 1,1‐diphenyl‐2‐picryl‐hydrazyl antioxidant, urease enzyme, and α‐glucosidase enzyme inhibition activities. Aloeverasides A (1) and B (2) displayed good urease enzyme inhibition activities (62 and 55%, resp.), as well as antioxidant activity in which aloeveraside A (1) had a value of 60% inhibition, while aloeveraside B (2) demonstrated a more potent antioxidant activity with 80% inhibition.
A Metal‐Free Synthesis of 2‐Alkyl(or Aryl) Thiomethyl‐2‐cyclohexenones from Cyclic Morita–Baylis–Hillman Bromides
Authors: Baioui Narjes , Abidi Ahlem , Rezgui Farhat 
Published: September 2016

Under mild conditions, an efficient and rapid S‐allylation of thiols with cyclic Morita–Baylis–Hillman (MBH) bromides without the need of a transition‐metal catalyst or an expensive additive is described herein. Treatment of the MBH bromides with various thiols or ethane‐1,2‐dithiol in the presence of Et3N regioselectively affords the corresponding 2‐alkyl(or aryl) thiomethyl‐2‐cyclohexenones or the perhydro benzo[1,4]dithiepinone, respectively, in moderate to good yields (40 – 73%). The reaction is rapid and carried out in THF at room temperature.
Remote sensing for restoration ecology: Application for restoring degraded, damaged, transformed, or destroyed ecosystems
Authors: Reif Molly K , Theel Heather J 
Published: September 2016

Abstract

Restoration monitoring is generally perceived as costly and time consuming, given the assumptions of successfully restoring ecological functions and services of a particular ecosystem or habitat. Opportunities exist for remote sensing to bolster the restoration science associated with a wide variety of injured resources, including resources affected by fire, hydropower operations, chemical releases, and oil spills, among others. In the last decade, the role of remote sensing to support restoration monitoring has increased, in part due to the advent of high-resolution satellite sensors as well as other sensor technology, such as lidar. Restoration practitioners in federal agencies require monitoring standards to assess restoration performance of injured resources. This review attempts to address a technical need and provides an introductory overview of spatial data and restoration metric considerations, as well as an in-depth review of optical (e.g., spaceborne, airborne, unmanned aerial vehicles) and active (e.g., radar, lidar) sensors and examples of restoration metrics that can be measured with remotely sensed data (e.g., land cover, species or habitat type, change detection, quality, degradation, diversity, and pressures or threats). To that end, the present article helps restoration practitioners assemble information not only about essential restoration metrics but also about the evolving technological approaches that can be used to best assess them. Given the need for monitoring standards to assess restoration success of injured resources, a universal monitoring framework should include a range of remote sensing options with which to measure common restoration metrics. Integr Environ Assess Manag 2017;13:614–630. Published 2016. This article is a US Government work and is in the public domain in the USA.

Key Points
Without post-implementation restoration monitoring, it is impossible to evaluate success, understand restoration recovery trajectories, or gather lessons learned that might better inform restoration science. Opportunities exist for remote sensing to bolster the restoration science associated with a wide variety of degraded, damaged, transformed, or destroyed ecosystems. This manuscript attempts to bring remote sensing technology to the forefront, while highlighting its applicability to site-specific restoration metrics for injured resources.
Classification of Wastewater Reuse for Agriculture: A Case Study in South Korea
Authors: Jeong Hanseok , Seong Chounghyun , Jang Taeil , Park Seungwoo 
Published: September 2016

AbstractThere are several types of wastewater reuse for agriculture, and an explicit classification is necessary before providing the proper management plan for the specific type. The objective of this study was to classify types of agricultural wastewater reuse for safe and sustainable wastewater irrigation. Three criteria were applied based on the reuse circumstances in Korea: (i) whether wastewater diluted with fresh water (direct/indirect); (ii) whether planned by government agencies (planned/unplanned); (iii) treatment level of wastewater (untreated wastewater/treated wastewater/reclaimed wastewater). The wastewater reuse for agriculture was classified into seven types. This study describes characteristics and a possible management practice for each reuse type, and also provides some representative cases. The proposed classification would contribute to a better understanding of agricultural wastewater reuse and help decision makers in both Korea and developing countries having agricultural similarity with Korea to provide optimal management plans. Copyright © 2016 John Wiley & Sons, Ltd.
Longitudinal study using voxel‐based relaxometry: Association between cartilage T1ρ and T2 and patient reported outcome changes in hip osteoarthritis
Authors: Pedoia Valentina , Gallo Matthew C. , Souza Richard B. , Majumdar Sharmila 
Published: September 2016

PurposeTo study the local distribution of hip cartilage T1ρ and T2 relaxation times and their association with changes in patient reported outcome measures (PROMs) using a fully automatic, local, and unbiased method in subjects with and without hip osteoarthritis (OA).Materials and MethodsThe 3 Tesla MRI studies of the hip were obtained for 37 healthy controls and 16 subjects with radiographic hip OA. The imaging protocol included a three‐dimensional (3D) SPGR sequence and a combined 3D T1ρ and T2 sequence. Quantitative cartilage analysis was compared between a traditional region of interest (ROI)‐based method and a fully automatic voxel‐based relaxometry (VBR) method. Additionally, VBR was used to assess local T1ρ and T2 differences between subjects with and without OA, and to evaluate the association between T1ρ and T2 and 18‐month changes PROMs.ResultsResults for the two methods were consistent in the acetabular (R = 0.79; coefficients of variation [CV] = 2.9%) and femoral cartilage (R = 0.90; CV = 2.6%). VBR revealed local patterns of T1ρ and T2 elevation in OA subjects, particularly in the posterosuperior acetabular cartilage (T1ρ: P = 0.02; T2: P = 0.038). Overall, higher T1ρ and T2 values at baseline, particularly in the anterosuperior acetabular cartilage (T1ρ: Rho = ‐0.42; P = 0.002; T2: Rho = ‐0.44; P = 0.002), were associated with worsening PROMS at 18‐month follow‐up.ConclusionVBR is an accurate and robust method for quantitative MRI analysis in hip cartilage. VBR showed the capability to detect local variations in T1ρ and T2 values in subjects with and without osteoarthritis, and voxel based correlations demonstrated a regional dependence between baseline T1ρ and T2 values and changes in PROMs.Level of Evidence: 1J. MAGN. RESON. IMAGING 2017;45:1523–1533
Ecologically sate and resource‐saving methods for recycling waste tungsten, niobium carbide‐cobalt cermets and extraction of tungsten and niobium from concentrates
Authors: Onischenko V. , Soloviev V. , Solianyk L. , Malyshev V. 
Published: September 2016

A range of potentials in which the cobalt–tungsten alloy phase is selectively dissolved in the solutions of phosphoric acid to form soluble compounds of these metals while the tungsten carbide phase remains in the precipitate is determined. It is shown that tungsten ores and concentrates are decomposed at temperatures of 1050 to 1100 °C in sodium chloride–sodium metasilicate melts to form two immiscible phases: halide–tungstate, which contains 96 to 99 %, and silicate, which contains 90 % of the ore components.
A grape seed procyanidin extract inhibits HDAC activity leading to increased Pparα phosphorylation and target‐gene expression
Authors: Downing Laura E. , Ferguson Bradley S. , Rodriguez Kelvin , Ricketts Marie‐Louise 
Published: September 2016

ScopeHistone deacetylases (HDACs) have emerged as epigenetic regulators of risk factors associated with the metabolic syndrome (MetS), and certain botanical extracts have proven to be potent HDAC inhibitors. Understanding the role of dietary procyanidins in HDAC inhibition is important in exploring the therapeutic potential of natural products.MethodsC57BL/6 mice were gavaged with vehicle (water) or grape seed procyanidin extract (GSPE, 250 mg/kg) and terminated 14 h later. Liver and serum were harvested to assess the effect of GSPE on HDAC activity, histone acetylation, Pparα activity and target‐gene expression, and serum lipid levels.ResultsGSPE increased histone acetylation and decreased Class I HDAC activity in vivo, and dose‐dependently inhibited recombinant HDAC2 and 3 activities in vitro. Accordingly, Pparα gene and phosphorylated protein expression were increased, as were target genes involved in fatty acid catabolism, suggesting increased Pparα activity. Serum fibroblast growth factor 21 (Fgf21) was elevated, and triglyceride levels were reduced by 28%.ConclusionGSPE regulates HDAC and Pparα activities to modulate lipid catabolism and reduce serum triglycerides in vivo.
A novel points system to predict the prognosis of ulnar neuropathy at the elbow
Authors: Beekman Roy , Zijlstra Wobbe , Visser Leo H. 
Published: September 2016

ABSTRACTIntroduction: In this study we aimed to identify prognostic factors of ulnar neuropathy at the elbow (UNE) and developed a scoring system to establish the prognosis. Methods: We collected baseline clinical, electrophysiologic, and ultrasonographic data from 2 cohorts. The outcomes for all patients were determined on follow‐up. Prognostic factors were determined using single and multiple variable analyses. A points system was developed to determine the risk for an unfavorable outcome. Results: Of the 220 patients with UNE 178 (81%) could be re‐evaluated. Four variables were retained in the prediction model for a points system. An unfavorable outcome was associated with right‐sided UNE, more severe weakness of the abductor digiti minimi (ADM), and more pronounced ulnar nerve thickening. A compound muscle action potential amplitude reduction across the elbow of ≥16% (particularly if ≥ 50%) was associated with a more favorable outcome. Conclusion: Outcome in UNE may be predicted by scoring 4 parameters. Muscle Nerve 55: 698–705, 2017
Monitoring and mechanisms of insecticide resistance in Chilo suppressalis (Lepidoptera: Crambidae), with special reference to diamides
Authors: Yao Rong , Zhao Dan‐Dan , Zhang Shuai , Zhou Li‐Qi , Wang Xin , Gao Cong‐Fen , Wu Shun‐Fan 
Published: September 2016

AbstractBACKGROUNDThe rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae), is one of the most economically important pests of rice in Asia. Chemical control remains the most efficient primary means for controlling this pest.RESULTSSignificant variations among field populations in their resistance to seven insecticides were observed. The populations exhibited LC50 values that ranged between 0.605 and 108.088 mg AI L−1 for chlorantraniliprole and between 0.046 and 3.919 mg AI L−1 for flubendiamide. The YY14 population collected from Yuyao in Zhejiang Province in 2014 showed a moderate resistance level to the two diamides, i.e. up to 77.6‐fold and 42.6‐fold for chlorantraniliprole and flubendiamide respectively. Synergism tests and biochemical assays showed no obvious correlations between diamide resistance and three detoxifying enzymes. Sequence comparison of the ryanodine receptor gene between the YY14 resistant population and susceptible population revealed that a glycine to glutamic acid substitution (G4910E) was presented in the YY14 population.CONCLUSIONG4910E mutation might be involved in the resistance evolution of C. suppressalis to the diamides. An appropriate insecticide resistance management programme should be established to maintain the effectiveness of the insecticides and to ensure sustainable management. © 2016 Society of Chemical Industry
Spectroscopic studies of Co2TiO4 and Co3O4 two‐phase composites
Authors: Nayak S. , Dasari K. , Joshi D. C. , Pramanik P. , Palai R. , Sathe V. , Chauhan R. N. , Tiwari N. , Thota S. 
Published: September 2016

AbstractIn this article, we report a comparative analysis of various spectroscopic studies including low‐temperature () Raman spectroscopy of cobalt‐orthotitanate () and tricobalt‐tetraoxide (), and their solid solutions of ( (100 wt.%)). For all the lower and intermediate compositions, five Raman‐active modes were recognized at , and that are associated with , , and phonon symmetries. Conversely, pure exhibits a broad spectrum of width without any signatures of (3) mode. At low‐temperatures (down to 25 K) the and peaks of both and shift toward the high‐frequency side with anomalies across the ferrimagnetic Néel temperature (K) and antiferromagnetic Néel temperature ( K), respectively. All the investigated samples exhibit two distinct bands at 576 () and 665 () in the Fourier transform infrared spectra recorded at K, associated with the vibrational stretching of the metal–oxygen bonds of length 195.8 pm (B–O) and 185.4 pm (A–O), respectively. The intensity of these sharp bands gradually decreases as the crystal structure transforms from normal‐spinel ( Å) to inverse‐spinel structure ( Å). The X‐ray photoelectron spectroscopy (XPS) studies revealed that the Ti was incorporated into the octahedral B‐sites of inverse‐spinel structure of . Interestingly, the XPS spectra of provide evidence of the trivalent character of Ti instead of tetravalent cationic configuration together with a weak character at the octahedral sites. These results are discussed in terms of the binding‐energy (BE) difference between the O‐1s and Ti‐2p ([O–Ti‐2p] = BE(O‐1s) −BE(Ti‐2p) and the mean chemical bond length . The peculiarities of all these results in consonance with the crystal‐structure (bond angles and bond lengths) and electron‐spin‐resonance studies are discussed in detail.