bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of lactoperoxidase system‐alginate coating on chemical, microbial, and sensory properties of chicken breast fillets during cold storage
Authors: Yousefi Mohammad , Farshidi Maryam , Ehsani Ali 
Published: January 2018

AbstractThe main aim of this study was to investigate the effect of alginate coating samples without lactoperoxidase system (A‐L 0) and alginate coating samples containing lactoperoxidase system (LPOS) at levels of 2% (A‐L 2), 4% (A‐L 4), and 6% (A‐L 6) on the shelf‐life of chicken breast fillets. Chemical tests, including total volatile basic nitrogen (TVB‐N), peroxide value (PV), pH, thiobarbituric acid reactive substances (TBARS), and free fatty acids (FFA), bacteriological tests of aerobic mesophilic bacteria (AMB), lactic acid bacteria (LAB), Pseudomonas aeruginosa, and Enterobacteriaceae and sensory analyses, including odor, transparency, color, and overall acceptability were performed. These coatings (especially A‐L 6), despite the effect on decreasing the formation of TVB‐N compounds (27.82 for control samples, and 21.72 mg N/100 g for A‐L 6 samples) had no significant impact (p > .05) on FFA, PV, and TBARS. The LPOS‐alginate coating was effective against Enterobacteriaceae, Pseudomonas aeruginosa, and aerobic mesophilic bacteria (AMB). Also, A‐L 6 samples got acceptable sensory results. Overall acceptability of A‐L 0 and A‐L 6 samples were 3.21 ± 0.36 and 6.23 ± 0.38, respectively on the last day. These scores are significantly different (p < .05). Generally, results demonstrated a positive effect of alginate‐LPOS coatings on shelf‐life extension of chicken breast fillets during cold storage.Practical applicationsConcerning the effect of alginate coating incorporated with lactoperoxidase system on controlling the bacterial growth and improving the shelf‐life of chicken breast fillets, such coating could has practical applications in increasing the food safety in meats and meat products.
Risk for the development of non‐alcoholic fatty liver disease: A prospective study
Authors: Wang Jinghua , Ma Liang , Chen Shenghui , Xu Lei , Miao Min , Yu Chaohui , Li Youming , Xu Chengfu 
Published: January 2018

AbstractBackground and AimNon‐invasive assessment was widely used to identify the risk of non‐alcoholic fatty liver disease (NAFLD) among individuals with increased metabolic risks. This study aimed to investigate the prospective relationship between ZJU index and the development of NAFLD in a Chinese population.MethodsA cohort of 6310 initially NAFLD‐free participants was enrolled in this prospective study. Abdominal ultrasound was used to diagnosis NAFLD. NAFLD incidence was calculated among participants with different baseline ZJU index quintiles. Cox proportional hazards regression analyses were conducted to calculate the risks for incident NAFLD.ResultsDuring 37 705 person‐year follow‐ups, 1071 incident NAFLD cases were identified. The baseline ZJU index was linear and positively correlated with NAFLD incidence. The incidence was 5.53, 11.75, 23.77, 43.28, and 85.60 cases per 1000 person‐year follow‐up for participants with baseline ZJU index in quintiles 1–5, respectively. Compared with participants with baseline ZJU index in quintile 1, the hazard ratios (95% confidence interval) for incident NAFLD were 2.092 (1.458–3.002), 4.094 (2.942–5.698), 7.095 (5.167–9.742), and 13.191 (9.684–17.968) for participants with baseline ZJU index in quintiles 2–5, respectively. Further analysis found that the changes of ZJU index during follow‐up was also independently associated with risk for incident NAFLD.ConclusionsBaseline ZJU index and absolute ZJU index changes independently predicts the risk for incident NAFLD in Chinese population.
Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated
Authors: Scavuzzo‐Duggan Tess R. , Chaves Arielle M. , Singh Abhishek , Sethaphong Latsavongsakda , Slabaugh Erin , Yingling Yaroslava G. , Haigler Candace H. , Roberts Alison W. 
Published: January 2018

AbstractCellulose synthases (CESAs) are glycosyltransferases that catalyze formation of cellulose microfibrils in plant cell walls. Seed plant CESA isoforms cluster in six phylogenetic clades, whose non‐interchangeable members play distinct roles within cellulose synthesis complexes (CSCs). A ‘class specific region’ (CSR), with higher sequence similarity within versus between functional CESA classes, has been suggested to contribute to specific activities or interactions of different isoforms. We investigated CESA isoform specificity in the moss, Physcomitrella patens (Hedw.) B. S. G. to gain evolutionary insights into CESA structure/function relationships. Like seed plants, P. patens has oligomeric rosette‐type CSCs, but the PpCESAs diverged independently and form a separate CESA clade. We showed that P. patens has two functionally distinct CESAs classes, based on the ability to complement the gametophore‐negative phenotype of a ppcesa5 knockout line. Thus, non‐interchangeable CESA classes evolved separately in mosses and seed plants. However, testing of chimeric moss CESA genes for complementation demonstrated that functional class‐specificity is not determined by the CSR. Sequence analysis and computational modeling showed that the CSR is intrinsically disordered and contains predicted molecular recognition features, consistent with a possible role in CESA oligomerization and explaining the evolution of class‐specific sequences without selection for class‐specific function.
Management of comedonal acne vulgaris with fixed‐combination topical therapy
Authors: Gold Michael H , Baldwin Hilary , Lin Tina 
Published: January 2018

SummaryBackgroundAcne vulgaris (acne) is the most common skin disease we see in dermatology practice. Clinically, it is characterized by a combination of open and closed comedones (formally referred to as noninflammatory lesions) and inflammatory papules and pustules. Comedonal acne is more typical in young adolescents, but can occur in combination with inflammatory papules and pustules at any time. Topical retinoids have long been advocated for the treatment of comedonal acne.AimsGiven the increasing recognition of the inflammatory nature of acne and the synergistic benefits seen with fixed combinations we review the latest clinical data to provide guidance on optimal management of comedonal acne.MethodsAn English language literature search of Medline, EMBASE, and the Web of Science using key terms (acne, comedonal, noninflammatory, clinical trials) was conducted, and relevant articles reviewed.ResultsComparative data is sparse, but we show the importance of fixed combinations with and without retinoids, where treatment benefits are comparable. Adapalene 0.1%‐benzoyl peroxide 2.5% gel has been shown to be comparable to clindamycin 1%‐benzoyl peroxide 5% gel, and adapalene 0.3%‐benzoyl peroxide 2.5% gel. A meta‐analysis suggested that clindamycin 1.2%‐benzoyl peroxide 2.5% gel was more effective than clindamycin‐benzoyl peroxide 5% gel in noninflammatory lesions, and two equivalent clinical programs suggest additional benefits of higher doses of benzoyl peroxide (3.75% vs 2.5%) in this fixed combination.ConclusionsClindamycin 1.2%‐benzoyl peroxide 3.75% gel may afford similar benefits to adapalene 0.3%‐benzoyl peroxide 2.5% gel in this sometimes difficult to treat patient population.
Metabolomic response to coffee consumption: application to a three‐stage clinical trial
Authors: Cornelis M. C. , Erlund I. , Michelotti G. A. , Herder C. , Westerhuis J. A. , Tuomilehto J. 
Published: January 2018

AbstractBackgroundCoffee is widely consumed and contains many bioactive compounds, any of which may impact pathways related to disease development.ObjectiveTo identify individual metabolite changes in response to coffee.MethodsWe profiled the metabolome of fasting serum samples collected from a previously reported single‐blinded, three‐stage clinical trial. Forty‐seven habitual coffee consumers refrained from drinking coffee for 1 month, consumed four cups of coffee/day in the second month and eight cups/day in the third month. Samples collected after each coffee stage were subject to nontargeted metabolomic profiling using UPLC‐ESI‐MS/MS. A total of 733 metabolites were included for univariate and multivariate analyses.ResultsA total of 115 metabolites were significantly associated with coffee intake (P < 0.05 and Q < 0.05). Eighty‐two were of known identity and mapped to one of 33 predefined biological pathways. We observed a significant enrichment of metabolite members of five pathways (P < 0.05): (i) xanthine metabolism: includes caffeine metabolites, (ii) benzoate metabolism: reflects polyphenol metabolite products of gut microbiota metabolism, (iii) steroid: novel but may reflect phytosterol content of coffee, (iv) fatty acid metabolism (acylcholine): novel link to coffee and (v) endocannabinoid: novel link to coffee.ConclusionsThe novel metabolites and candidate pathways we have identified may provide new insight into the mechanisms by which coffee may be exerting its health effects.
Role of clarity of other's feelings for dyadic coping
Authors: LEUCHTMANN LORENA , ZEMP MARTINA , MILEK ANNE , NUSSBECK FRIDTJOF W. , BRANDSTÄTTER VERONIKA , BODENMANN GUY 
Published: January 2018

AbstractDyadic coping has repeatedly been associated with positive outcomes in intimate relationships. However, less is known about the prospective predictors of dyadic coping. This study investigates clarity of other's feelings (CoF) as a potential predictor of supportive dyadic coping in a longitudinal study. In a sample of 368 couples, self‐reported CoF and supportive dyadic coping perceived by the partner were assessed annually over 3 years. Results revealed that interpersonal differences in men and women's CoF are positively associated with interpersonal differences in supportive dyadic coping. Moreover, interpersonal differences in men's CoF predicted long‐term intrapersonal changes in supportive dyadic coping of both partners. Couple intervention programs might strengthen couple's dyadic coping skills by targeting men's understanding of their partner's feelings.
Extracorporeal photopheresis as second‐line treatment therapy in life‐threatening primary graft dysfunction following lung transplantation
Authors: Robinson Cécile A , Inci Ilhan , Naegeli Mirjam , Murer Christian , Schuurmans Macé M , Urosevic‐Maiwald Mirjana , Schüpbach Reto , Weder Walter , Benden Christian 
Published: January 2018

AbstractECP is an established “second‐line” treatment for CLAD/BOS. Recently, ECP was used for the first time in an adolescent CF patient as a “second‐line” treatment therapy in life‐threatening primary graft dysfunction following lung transplantation who deteriorated despite extensive treatment including ECMO and ATG. Within 10 days after initiation of ECP twice weekly, allograft function and clinical status improved significantly and the patient was weaned from mechanical ventilation support. ECP has been continued every 2 weeks since. Two hundred days after lung transplantation, the patient has an acceptable allograft function (FEV1 67%) and no signs of allograft rejection. We advocate that use of ECP and its immunomodulatory effects should be evaluated in the early period following lung transplantation.
Leishmania amazonensis induces modulation of costimulatory and surface marker molecules in human macrophages
Authors: Costa S. S. , Fornazim M. C. , Nowill A. E. , Giorgio S. 
Published: January 2018

SummaryManipulation of costimulatory and surface molecules that shape the extent of immune responses by Leishmania is suggested as one of the mechanisms of evading the host's defences. The experiments reported here were designed to evaluate the expressions of CD11b, CD11c, CD14, CD18, CD54, CD80, CD86, CD206, MHC class II and TLR‐2 (Toll‐like receptor 2) in human macrophages infected with L. amazonensis. Phenotypic evaluation revealed a negative modulation in CD11b, CD11c, CD14, CD18, CD54 and MHC class II molecules, depending on the level of infection. The results showed that as early as 1 hour after infection no reduction in marker expression occurs, whereas after 24 hours, downregulation of these molecules was observed in macrophages. No significant changes were observed in the expressions of CD80, CD86, CD206 and TLR2. Evidence of the differential modulation of markers expression and that after parasite uptake no reduction in surface marker expression occurs indicates that parasite internalization is not involved in the phenomena of down‐modulation.
Pregnancy Weight Gain by Gestational Age in Women with Uncomplicated Dichorionic Twin Pregnancies
Authors: Hutcheon Jennifer A. , Platt Robert W. , Abrams Barbara , Braxter Betty J. , Eckhardt Cara L. , Himes Katherine P. , Bodnar Lisa M. 
Published: January 2018

AbstractBackgroundTwin pregnancies are at increased risk for adverse outcomes and are associated with greater gestational weight gain compared to singleton pregnancies. Studies that disentangle the relationship between gestational duration, weight gain and adverse outcomes are needed to inform weight gain guidelines. We created charts of the mean, standard deviation and select percentiles of maternal weight gain‐for‐gestational age in twin pregnancies and compared them to singleton curves.MethodsWe abstracted serial prenatal weight measurements of women delivering uncomplicated twin pregnancies at Magee‐Womens Hospital (Pittsburgh, PA, 1998–2013) and merged them with the hospital's perinatal database. Hierarchical linear regression was used to express pregnancy weight gain as a smoothed function of gestational age according to pre‐pregnancy BMI category. Charts of week‐ and day‐specific values for the mean, standard deviation, and percentiles of maternal weight gain were created.ResultsPrenatal weight measurements (median: 11 [interquartile range: 9, 13] per woman) were available for 1109 women (573 normal weight, 287 overweight, and 249 obese). The slope of weight gain was most pronounced in normal weight women and flattened with increasing pre‐pregnancy BMI (e.g. 50th percentiles of 6.8, 5.7, and 3.6 kg at 20 weeks and 19.8, 18.1, and 14.4 at 37 weeks in normal weight, overweight, and obese women, respectively). Weight gain patterns in twins diverged from singletons after 17–19 weeks.ConclusionsOur charts provide a tool for the classification of maternal weight gain in twin pregnancies. Future work is needed to identify the range of weight gain associated with optimal pregnancy health outcomes.
T‐cell cross‐reactivity may explain the large variation in how cancer patients respond to checkpoint inhibitors
Authors: Sioud Mouldy 
Published: January 2018

AbstractThe therapeutic use of the immune system to specifically attack tumours has been a long‐standing vision among tumour immunologists. Recently, the use of checkpoint inhibitors to turn‐off immunosuppressive signals has proven to be effective in enhancing T‐cell reactivity against patient‐specific neoantigens, resulting from somatic mutations. Several of the identified T‐cell epitopes share similarity with common bacterial and viral antigens, suggesting the involvement of pre‐existing microbial cross‐reactive T cells in rapid and durable tumour regression seen in some patients. This notion of T‐cell cross‐reactivity is further supported by the findings that intestinal bacteria can influence checkpoint‐blockade therapy. Moreover, early data indicate the presence of such T cells in long‐term survival breast cancer patients. This review highlights the main challenges for cancer immunotherapy and discusses the potential contribution of T‐cell cross‐reactivity in cancer immunotherapy and whether it can be used as a biomarker to predict the responsiveness to checkpoint inhibitors.
Platelet sequestration with a new‐generation autotransfusion device
Authors: Seyfried Timo F. , Streithoff Fabian , Gruber Michael , Unterbuchner Christoph , Zech Nina , Kieninger Martin , Hansen Ernil 
Published: January 2018

BACKGROUNDAutologous platelet‐rich plasma (PRP) can be used either to prevent platelets (PLTs) from lesions during extracorporeal circulation or for wound therapy, when processed into PLT gel. The aim of this study was to evaluate the PLT sequestration abilities of a new‐generation autotransfusion device.STUDY DESIGN AND METHODSIn this experimental study the discontinuous, new‐generation autotransfusion device XTRA was evaluated using fresh donor blood. The blood was processed in four different size bowls (X55, X125, X175, X225 [bowls' size in mL]) using the device's built‐in “PLT sequestration program.” PLT functionality was tested using aggregometry; for PLT activation, β‐thromboglobulin (β‐TG) and soluble P‐selectin levels were determined. Cell damage was assessed by a morphology score and hypotonic shock response. Additionally, PLTs were tested after 6 hours to identify storage lesions.RESULTSPlatelet recovery in the PRP ranged from 39% to 64% and averaged 6.2‐fold PLT enrichment as defined by the increase in PLT concentration. The preparation caused minimal cell damage and a decrease in cell function by only 10%, but a slight activation was observed amounting to 9% of the maximal β‐TG release. The efficiency of the preparation, represented by the PLT recovery rate, increased in a linear fashion with the increasing bowl sizes being tested. After 6 hours of storage the prepared PLTs showed an additional 9% loss in function, but only 4% decrease in viability.CONCLUSIONThe autotransfusion device XTRA was capable of high‐quality perioperative PRP preparation, and the bowl size was found to have an influence on the efficiency of the preparation.
Accurate costs of blood transfusion: a microcosting of administering blood products in the United Kingdom National Health Service
Authors: Stokes Elizabeth A. , Wordsworth Sarah , Staves Julie , Mundy Nicola , Skelly Jane , Radford Kelly , Stanworth Simon J. 
Published: January 2018

BACKGROUNDIn an environment of limited health care resources, it is crucial for health care systems which provide blood transfusion to have accurate and comprehensive information on the costs of transfusion, incorporating not only the costs of blood products, but also their administration. Unfortunately, in many countries accurate costs for administering blood are not available. Our study aimed to generate comprehensive estimates of the costs of administering transfusions for the UK National Health Service.STUDY DESIGN AND METHODSA detailed microcosting study was used to cost two key inputs into transfusion: transfusion laboratory and nursing inputs. For each input, data collection forms were developed to capture staff time, equipment, and consumables associated with each step in the transfusion process. Costing results were combined with costs of blood product wastage to calculate the cost per unit transfused, separately for different blood products. Data were collected in 2014/15 British pounds and converted to US dollars.RESULTSA total of 438 data collection forms were completed by 74 staff. The cost of administering blood was $71 (£49) per unit for red blood cells, $84 (£58) for platelets, $55 (£38) for fresh‐frozen plasma, and $72 (£49) for cryoprecipitate.CONCLUSIONSBlood administration costs add substantially to the costs of the blood products themselves. These are frequently incurred costs; applying estimates to the blood components supplied to UK hospitals in 2015, the annual cost of blood administration, excluding blood products, exceeds $175 (£120) million. These results provide more accurate estimates of the total costs of transfusion than those previously available.
Neural crest‐derived cells migrate from nerve to participate in Achilles tendon remodeling
Authors: Xu Kang , Pan Xin , Qiu Xuefeng , Wang Dong , Dong Nianguo , Yang Li , Li Song 
Published: January 2018

AbstractDuring tendon injury, nerve ingrowth is one of the earliest events of tendon repair and remodeling. Since peripheral neurons and associated cells are mostly derived from neural crest (NC) cells, we sought to investigate the role of NC‐derived cells in tendon regeneration. Thus, we used Sox10‐Cre/ROSA26‐Flox‐Red Fluorescent Protein (RFP) transgenic mice to trace these cells during tendon regeneration. After 4 weeks of Achilles tendon rupture, the injured tendon tissues were harvested for immunohistological analyses, cell isolation, and phenotype identification. In addition, the tenocytes were co‐cultured with RFP labeled cells to examine cellular functions. Following the injury, a significant number of RFP‐labeled cells penetrated into the wound site and reached a peak (∼30% of cells) after 2 weeks, and then stabilized at a level of approximately 20%. Interestingly, 36.9% RFP labeled cells in the injured area expressed Tuj1, suggesting that most of the cells are peripheral neurons. Some RFP+/Tuj1+ cells were also found adjacent to newly formed blood vessels in the tendon. Importantly, the existing neuropeptide Y (NPY) and neuropeptide Y receptor (NPYr) in the invading nerve and blood vessels were directly correlated. In addition, there were also RFP+ cells (∼30%) negative for neuronal markers but positive for fibroblast markers, that is, FAP (34.7%) and Vimentin (Vmt) (27.2%), and approximately 10% positive for Sox10. Indeed, many RFP+ cells isolated from the ruptured Achilles tendon showed long spindle shapes and expressed fibroblast phenotypic markers FSP1 and FAP. Part of the Sox10+RFP‐labeled cells exhibited osteogenic and adipogenic differentiation ability. It is concluded that after Achilles tendon injury, nerves sprout into the wound site. The NC‐derived Vmt+/FAP+ mesenchymal cells and peripheral nerves participate in tendon regeneration.