bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: MicroRNA‐106b overexpression alleviates inflammation injury of cardiac endothelial cells by targeting BLNK via the NF‐κB signaling pathway
Authors: An Zhe , Yang Guang , Nie Wei , Ren Jin , Wang Dan 
Published: November 2017

AbstractWe aim to investigate whether microRNA‐106b (miR‐106b) affects the inflammation injury of cardiac endothelial cells (ECs) by targeting B‐cell linker (BLNK) via the NF‐κB signaling pathway. Human cardiac microvascular endothelial cells (HCMECs) were assigned into the control, hypoxia/reoxygenation (H/R), negative control (NC), pyrrolidine dithiocarbamate (PDTC), miR‐106b mimic, miR‐106b inhibitor, and si‐BLNK, and miR‐106b inhibitor+si‐BLNK groups. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blotting were conducted for miR‐106b expression and expressions of BLNK, interleukin (IL)‐6, IL‐1β, tumor necrosis factor (TNF)‐α, NF‐κB, pIκBα, BTK, and PLC‐γ2. Enzyme‐linked immunosorbent assay was applied for levels of IL‐6, IL‐10, and TNF‐α; cell counting Kit‐8 assay for cell proliferation; and flow cytometry for cell cycle and ensuing apoptosis. In‐vitro tube formation assay was performed for tube formation ability. Dual‐luciferase reporter assay revealed that BLNK was verified as the target gene of miR‐106b. Compared with the H/R and NC groups, the PDTC, miR‐106b mimic, and si‐BLNK groups had declined expressions of IL‐6, IL‐1β, TNF‐α, BTK, PLC‐γ2, NF‐κB p105/p50, and pIκBα as well as levels of L‐6 and TNF‐α, but contrarily elevated levels of NF‐κB p105/p50 and IL‐10. The PDTC, miR‐106b mimic, and si‐BLNK groups had less cell number in G0/G1 phase but higher cell count in both S and G2 phases, decreased cell apoptosis but increased proliferation and tube formation ability, while opposite trends were observed in the miR‐106b inhibitor group. Our findings indicated that the overexpression of miR‐106b alleviated the inflammation injury of cardiac ECs by targeting BLNK via the NF‐κB signaling pathway.
Design, expression and evaluation of novel chimeric protein constructed from colorectal tumor‐associated antigen
Authors: Veisi Malekshahi Ziba , Amani Jafar , Negahdari Babak , Rajabibazl Masoumeh , Ebrahimizadeh Walead 
Published: November 2017

AbstractThere were 134,000 new diagnosis and 49,000 deaths in 2016 due to colorectal cancer. Similar to most cancers, early diagnosis increases the chance of successful treatment. Detection of tumor‐associated antigens or the immune response against such markers is one of the most common methods of diagnosis. In that regard, we aimed to design and express a chimeric protein from the most common tumor‐associated antigens in colorectal cancer and assess its ability to detect the immune response in comparison with the parental tumor‐associated antigens in patient's sera. Through bioinformatics approaches a chimeric protein from carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA19‐9) was designed and expressed in E. coli (BL21DE3). Proper folding, expression levels and immune reactivity were assessed by western blot, ELISA and immunohistochemistry. Recombinant proteins functionality and immune reactivity were confirmed by ELISA and Western blot. Results showed that recombinant CEA, recombinant CA19.9 and chimeric protein of CEA‐ CA19.9 have strong reactivity with antibodies in the sera of colorectal cancer patients, whereas no reactivity was seen with the sera of healthy volunteers. Significantly stronger immune reactivity was seen with the chimeric protein than each of the CEA or CA19.9 alone. Overall, it was concluded that the designed recombinant proteins in this study could be used to detect autoantibodies produced against the colorectal tumor‐associated antigens. The chimeric CEA‐CA19.9 protein shows a stronger reactivity with the sera antibodies of colorectal cancer patients that CEA or CA19.9 alone.
Motivation and treatment credibility predict alliance in cognitive behavioral treatment for youth with anxiety disorders in community clinics
Authors: Fjermestad K. W. , Lerner M. D. , McLeod B. D. , Wergeland G. J. H. , Haugland B. S. M. , Havik O. E. , Öst L.‐G. , Silverman W. K. 
Published: November 2017

AbstractObjectiveWe examined whether motivation and treatment credibility predicted alliance in a 10‐session cognitive behavioral treatment delivered in community clinics for youth anxiety disorders.MethodNinety‐one clinic‐referred youths (meanage = 11.4 years, standard deviation = 2.1, range 8–15 years, 49.5% boys) with anxiety disorders‐rated treatment motivation at pretreatment and perceived treatment credibility after session 1. Youths and therapists (YT) rated alliance after session 3 (early) and session 7 (late). Hierarchical linear models were applied to examine whether motivation and treatment credibility predicted YT early alliance, YT alliance change, and YT alliance agreement.ResultsMotivation predicted high early YT alliance, but not YT alliance change or alliance agreement. Youth‐rated treatment credibility predicted high early youth alliance and high YT positive alliance change, but not early therapist alliance or alliance agreement.ConclusionConclusion Efforts to enhance youth motivation and treatment credibility early in treatment could facilitate the formation of a strong YT alliance.
Towards a methodology for recovering K‐struvite from manure
Authors: Tarragó Elena , Ruscalleda Maël , Colprim Jesús , Balaguer M Dolors , Puig Sebastià 
Published: November 2017

AbstractBACKGROUNDThis study provides a methodology for recovering two essential macronutrients (potassium and phosphorus) from the liquid fraction of manure. This methodology paves the way for K‐struvite recovery from manure by determining the suitable operational conditions (pH and temperature).RESULTSThe theoretical operational conditions were established by modelling and experimentally adjusted with manure, achieving the highest recoveries (80% of P‐recovery) at pH 10 and 38 °C when using a 10% manure solution. Then, the suitable operational conditions were evaluated using 0–10–50–100% manure solutions. The lowest percentages of manure (0–10%) favoured the co‐precipitation of other products besides K‐struvite (magnesium phosphate and magnesium hydroxide); and at the highest percentages of manure (50–100%), all the phosphate was recovered as K‐struvite, but also the co‐precipitation of magnesium hydroxide due to the Mg/P molar ratio applied (Mg/P = 2). Suspended particles acted as nuclei for heterogeneous nucleation and favoured the aggregation of crystals resulting in a star‐asterisk form.CONCLUSIONThe viability of the process was demonstrated, this study being the first proof‐of‐concept of K‐struvite recovery from complex waste streams. © 2017 Society of Chemical Industry
Gastric venous congestion and bleeding in association with total pancreatectomy
Authors: Nakao Akimasa , Yamada Suguru , Fujii Tsutomu , Tanaka Haruyoshi , Oshima Kenji , Oshima Yukiko , Iede Kiyotsugu , Kobayashi Hironobu , Kimura Yasunori , Kodera Yasuhiro 
Published: November 2017

AbstractBackgroundGastric venous congestion and bleeding in association with total pancreatectomy (TP) were evaluated.MethodsThirty‐eight patients of TP were retrospectively analyzed. TP was classified as TP with distal gastrectomy (TPDG), pylorus‐preserving TP (PPTP), subtotal stomach‐preserving TP (SSPTP), and TP with segmental duodenectomy (TPSD).ResultsPortal vein or superior mesenteric vein resection and reconstruction was performed in 24 patients (62.2%). Gastric bleeding occurred immediately after tumor resection in one of eight patients who underwent SSPTP, and urgent anastomosis between the right gastroepiploic and left ovarian vein stopped the bleeding. Another case of gastric bleeding was observed a few hours after TP in one of nine patients who underwent PPTP, and hemostasis was achieved after conservative therapy. Gastric bleeding was not observed in 16 patients who underwent TPDG and five who underwent TPSD. Some patients underwent preservation of gastric drainage veins (left gastric vein, right gastric vein, or right gastroepiploic vein). Neither patient with bleeding underwent preservation of a gastric drainage vein.ConclusionsTo preserve the subtotal or whole stomach when performing TP, one of the gastric drainage veins should undergo preservation or reconstruction, and anastomosis between the right gastroepiploic vein and left ovarian vein may be beneficial.
An immunoinformatics approach to define T cell epitopes from polyketide and non‐ribosomal peptide synthesis proteins of Mycobacterium tuberculosis as potential vaccine candidates
Authors: Dhivya S. , Baskar V. , Kumar S. R. , Sathishkumar R. 
Published: November 2017

AbstractThe role of polyketide and non‐ribosomal proteins from the class of small molecule metabolism of Mycobacterium tuberculosis is well documented in envelope organization, virulence, and pathogenesis. Consequently, the identification of T cell epitopes from these proteins could serve to define potential antigens for the development of vaccines. Fourty‐one proteins from polyketide and non‐ribosomal peptide synthesis of small molecule metabolism proteins of M tuberculosis H37Rv were analyzed computationally for the presence of HLA class I binding nanomeric peptides. All possible overlapping nanomeric peptide sequences from 41 small molecule metabolic proteins were generated through in silico and analyzed for their ability to bind to 33 alleles belonging to A, B, and C loci of HLA class I molecule. Polyketide and non‐ribosomal protein analyses revealed that 20% of generated peptides were predicted to bind HLA with halftime of dissociation T1/2 ≥ 100 minutes, and 77% of them were mono‐allelic in their binding. The structural bases for recognition of nanomers by different HLA molecules were studied by structural modeling of HLA class I‐peptide complexes. Pathogen peptides that could mimic as self‐peptides or partially self‐peptides in the host were excluded using a comparative study with the human proteome; thus, subunit or DNA vaccines will have more chance of success.
Why minority interests may be encouraged by majority regulation: A case study illustrated by slow money movement
Authors: Ashta Arvind 
Published: November 2017

AbstractMinorities seeking majority legislations need to communicate the increased option set and limit the downside risk for the system. Social movements urging for change require institutional support including enabling legislation. Public policy support may require associating with similar movements or other stakeholders. Loss aversion, downside risk, crisis, and real options may be reasons for majorities to approve minority legislation.
Properties and Phase Structure of Polycaprolactone‐Based Segmented Polyurethanes with Varying Hard and Soft Segments: Effects of Processing Conditions
Authors: Imre Balazs , Gojzewski Hubert , Check Casey , Chartoff Richard , Vancso G. Julius 
Published: November 2017

AbstractA series of segmented polycaprolactone polyurethane (PU) polymers is synthesized. One set of polymers ranges in composition from 0 to 100 wt% hard segments (HSs). The syntheses are carried out in solution and the polymers are melt‐processed by compression molding. Another subset of polymers is formed in bulk from a blocked isocyanate prepolymer. The blocked polymer's thermal and mechanical properties are compared with the melt‐processed materials. The emphasis in this paper is on the effects of varying the chemical structures of the PUs on their phase structures and physical cross‐linking due to nanocrystalline hard domains. The thermal properties indicate that nanophase separation and the formation of hard domains occur at HS contents above ≈8 wt%. Property differences resulting from varying the hard segment amounts are directly related to differences in morphology at the nanoscale. Atomic force microscopy images show that the best elastomeric mechanical properties are found when nanocrystallites are 4–5 nm in size.
Photocontrolled Synthesis of Abiotic Sequence‐Defined Oligo(Phosphodiester)s
Authors: König Niklas Felix , Telitel Sofia , Poyer Salomé , Charles Laurence , Lutz Jean‐François 
Published: November 2017

AbstractA photoregulated phosphoramidite iterative process is studied for the synthesis of non‐natural, digitally encoded oligo(phosphodiester)s. The oligomers are prepared using two reactive phosphoramidite monomers containing a 2‐(2‐nitrophenyl)propoxycarbonyl (NPPOC) protected OH group. The stepwise synthesis is performed on an OH‐functional soluble polystyrene support, which allows recycling by precipitation in a nonsolvent. Repeating cycles involving phosphoramidite coupling, oxidation of phosphite to phosphate, and NPPOC deprotection by light irradiation at λ = 365 nm are performed in order to prepare oligomers with different lengths and sequences. Synthesis is conducted on a micromolar scale and good recycling yields are obtained in all cases. The use of a soluble polymer support allows an in‐depth characterization of the NPPOC photo‐deprotection step by 1H NMR, UV spectroscopy, and size exclusion chromatography, and thus identification of optimal synthesis conditions. After cleavage from the support, the oligo(phosphodiester)s are characterized by tandem mass spectrometry, which confirms preparation of uniform sequence‐coded oligomers.
A novel mica‐based composite with hybrid aramid fibers for electrical insulating applications: largely improved mechanical properties and moisture resistance
Authors: Zhao Yongsheng , Dang Wanbin , Lu Zhaoqing , Wang Lamei , Si Lianmeng , Zhang Meiyun 
Published: November 2017

AbstractThe fabrication of mica‐based composites with good mechanical properties is very important especially for electrical insulation applications. In this study, a new aramid fibrid along with chopped fibers was introduced into a mica system, and the composites were prepared using a papermaking machine. It was found that the mechanical properties of both fibrid–mica composite (F‐M paper) and fiber/fibrid–mica composite (A‐M paper) were largely improved in comparison with the reference sample (M paper) with an increase in the content of fibrid or hybrid floc/fibrid. This enhancement can be attributed to two mechanisms: (1) enhanced interfacial adhesion derived from the addition of fibrids with good flexibility and wrapping properties and (2) significant reinforcing due to the frameworks originating from the addition of chopped flocs with high modulus and rigid‐rod morphology. Interestingly, a synergetic effect of enhanced interfacial adhesion and reinforcing could also be observed when both flocs and fibrids were introduced, which further improved the mechanical properties. Furthermore, the addition of aramid fibrid greatly enhanced the moisture resistance, which expands the potential for mica‐based composites. Thus, we successfully fabricated a mica‐based composite for electrical insulation with good mechanical properties, high end‐use temperature and excellent moisture resistance by adding hybrid aramid fibers. © 2017 Society of Chemical Industry
Influence of the precursor layer composition and deposition processes on the electronic quality of liquid phase crystallized silicon absorbers
Authors: Amkreutz Daniel , Preissler Natalie , Thi‐Trinh Cham , Trahms Martina , Sonntag Paul , Schlatmann Rutger , Rech Bernd 
Published: November 2017

AbstractLiquid phase crystallization using line‐shaped energy sources such as CW‐diode lasers or electron beams has proven to form mc‐Si layers on borosilicate or borosilicate/aluminosilicate glass that exhibit wafer equivalent grain sizes and electronic quality. In this work, we characterize the impact of the employed dielectric interlayer stack sandwiched between glass and absorber on the electronic quality. For this purpose, we investigate a large variety of test cell results achieved in the past on different interlayer stacks composed of silicon oxide, silicon nitride as well as silicon oxynitride deposited by means of plasma enhanced chemical vapour deposition or plasma oxidation and employ i(v), SunsVoc, quantum efficiency measurements, and photoluminescence imaging to assess the electronic properties of the crystallized absorbers. The results are compared with state‐of the art interdigitated back‐contact cells and literature values. Based on these findings, we conclude that at the present state of interlayer , the bulk quality imposes the limits in cell efficiency and investigates potential approaches to increase the bulk quality of LPC‐Si absorbers.
[11C]arachidonic acid incorporation measurement in human brain: Optimization for clinical use
Authors: Zanderigo Francesca , Kang Yeona , Kumar Dileep , Nikolopoulou Anastasia , Mozley P. David , Kothari Paresh J. , He Bin , Schlyer David , Rapoport Stanley I. , Oquendo Maria A. , Vallabhajosula Shankar , Mann J. John , Sublette M. Elizabeth 
Published: November 2017

AbstractArachidonic acid (AA) is involved in signal transduction, neuroinflammation, and production of eicosanoid metabolites. The AA brain incorporation coefficient (K*) is quantifiable in vivo using [11C]AA positron emission tomography, although repeatability remains undetermined. We evaluated K* estimates obtained with population‐based metabolite correction (PBMC) and image‐derived input function (IDIF) in comparison to arterial blood‐based estimates, and compared repeatability. Eleven healthy volunteers underwent a [11C]AA scan; five repeated the scan 6 weeks later, simulating a pre‐ and post‐treatment study design. For all scans, arterial blood was sampled to measure [11C]AA plasma radioactivity. Plasma [11C]AA parent fraction was measured in 5 scans. K* was quantified using both blood data and IDIF, corrected for [11C]AA parent fraction using both PBMC (from published values) and individually measured values (when available). K* repeatability was calculated in the test‐retest subset. K* estimates based on blood and individual metabolites were highly correlated with estimates using PBMC with arterial input function (r = 0.943) or IDIF (r = 0.918) in the subset with measured metabolites. In the total dataset, using PBMC, IDIF‐based estimates were moderately correlated with arterial input function‐based estimates (r = 0.712). PBMC and IDIF‐based K* estimates were ∼6.4% to ∼11.9% higher, on average, than blood‐based estimates. Average K* test‐retest absolute percent difference values obtained using blood data or IDIF, assuming PBMC for both, were between 6.7% and 13.9%, comparable to other radiotracers. Our results support the possibility of simplified [11C]AA data acquisition through eliminating arterial blood sampling and metabolite analysis, while retaining comparable repeatability and validity.
In vitro biotransformation of pyrrolizidine alkaloids in different species. Part I: Microsomal degradation
Authors: Kolrep Franziska , Numata Jorge , Kneuer Carsten , Preiss-Weigert Angelika , Lahrssen-Wiederholt Monika , Schrenk Dieter , These Anja 
Published: November 2017

Abstract
Pyrrolizidine alkaloids (PA) are secondary metabolites of certain flowering plants. The ingestion of PAs may result in acute and chronic effects in man and livestock with hepatotoxicity, mutagenicity, and carcinogenicity being identified as predominant effects. Several hundred PAs sharing the diol pyrrolizidine as a core structure are formed by plants. Although many congeners may cause adverse effects, differences in the toxic potency have been detected in animal tests. It is generally accepted that PAs themselves are biologically and toxicologically inactive and require metabolic activation. Consequently, a strong relationship between activating metabolism and toxicity can be expected. Concerning PA susceptibility, marked differences between species were reported with a comparatively high susceptibility in horses, while goat and sheep seem to be almost resistant. Therefore, we investigated the in vitro degradation rate of four frequently occurring PAs by liver enzymes present in S9 fractions from human, pig, cow, horse, rat, rabbit, goat, and sheep liver. Unexpectedly, almost no metabolic degradation of any PA was observed for susceptible species such as human, pig, horse, or cow. If the formation of toxic metabolites represents a crucial bioactivation step, the found inverse conversion rates of PAs compared to the known susceptibility require further investigation.
In-Situ Synchrotron X-ray Diffraction Studies on Effects of Plastic and Elastic Loading on bcc Phase Transformations of a 3rd Generation 1 GPa Advanced High Strength Steel
Authors: Eftekharimilani P. , Huizenga R. M. , Kim B. , Bernasconi A. , Hermans M. J. M. 
Published: November 2017

Abstract
In this paper, we describe the effects of mechanical loading on bcc-to-bcc phase transformations of an Advanced High Strength Steel during cooling. In-situ synchrotron diffraction was employed to measure time–temperature–load diffraction patterns. Calculations were made of the volume fractions of the phases, the transformation kinetics, and the austenite lattice parameter during cooling and simultaneous loading. In addition, volume fractions and lattice parameters of retained austenite at room temperature under different loading conditions were obtained. The results show that applying a load during cooling of the fcc phase significantly increases the volume fraction of a bcc phase before the start of the martensitic transformation. The kinetics of phase transformations were affected by the applied loads. The volume fraction and lattice parameter of retained austenite at room temperature vary in different samples and the highest retained austenite and the largest lattice parameter were obtained in the sample subjected to the highest load.
Three Dimensions of Change in School Segregation: A Grade-Period-Cohort Analysis
Authors: Fiel Jeremy E. , Zhang Yongjun 
Published: November 2017

AbstractThis study uses the first age-period-cohort (APC) analysis of segregation to examine changes in U.S. public school segregation from 1999–2000 to 2013–2014. APC analyses disentangle distinct sources of change in segregation, and they account for grade effects that could distort temporal trends if grade distributions change over time. Findings indicate that grade effects are substantial, drastically reducing segregation at the transition to middle school and further at the transition to high school. These grade effects do not substantially distort the analysis of recent trends, however, because grade distributions were sufficiently stable. Black-white segregation was stagnant overall, while Hispanic-white segregation declined modestly. In both cases, declines across periods were offset by increases across cohorts. Further analyses reveal variation in these trends across metropolitan and nonmetropolitan areas, regions, and areas with different histories of desegregation policy.
A matter of fact? Adolescents' assumptions about crime, laws, and authority and their domain‐specific beliefs about punishment
Authors: Oosterhoff Benjamin , Shook Natalie J. , Metzger Aaron 
Published: November 2017

ABSTRACTThis study examined adolescents' beliefs about the amount of punishment individuals should receive for violating different laws and whether these beliefs are connected with their informational assumptions (i.e., perceived facts) about crime, laws, and authority. American adolescents (N = 340; Mage = 16.64, 58.2% female) reported their judgments concerning the appropriate punishment for violating laws regulating domain‐specific behaviors and their informational assumptions regarding the prevalence and causes of crime, beliefs that authority is knowledgeable, and the purpose of punishment. Greater internal attributions for crime was associated with stronger punishment judgments for violating laws that regulate moral and conventional issues. Greater beliefs that punishment teaches right from wrong was associated with stronger punishment judgments for violating laws that regulate drug‐related prudential issues, and lower punishment judgments for violating laws that regulate personal issues. Greater beliefs that authorities are more knowledgeable than others was associated with stronger punishment judgments for violating laws that regulate personal issues.