bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atividade Antibacteriana de Extratos de Chlorella sorokiniana Obtidos por Extração com Diferentes Solventes Orgânicos
Authors: Teixeira Simões da Silva Iasmin , César Richter Nogueira Caio , Da Silva Josemeyre Bonifácio , Rodrigo Ito Morioka Luiz , Hiroshi Suguimoto Hélio 
Published: October 2024

O estudo teve como objetivo avaliar qualitativamente o efeito inibitório de extratos de Chlorella sorokiniana obtidos pela extração com diferentes solventes orgânicos com polaridades crescentes em bactérias patogênicas. A microalga C. sorokiniana cepa IPR 7104 foi liofilizada e submetida às extrações com os respectivos solventes: hexano, diclorometano, acetato de etila e metanol para posterior ensaio inibitório. Para o teste de inibição, as bactérias Escherichia coli, Salmonella enterica e Serratia marcescens foram cultivadas em meio Müeller-Hinton e a partir disso, foi preparado um inóculo bacteriano, diluído na proporção 1/100. 195 µL de inóculo bacteriano foi acondicionado em tubo de ensaio com 5 µL dos diferentes extratos de C. sorokiniana. O conteúdo dos tubos foi esgotado em placas e avaliado. Os resultados do ensaio antibacteriano com os extratos da microalga C. sorokiniana obtidos pela extração com diferentes solventes indicaram que somente o extrato hexânico na concentração de 500 µg/mL apresentou inibição para a bactéria Serratia marcescens. A concentração inibitória mínima do extrato hexânico de C. sorokiniana é de 400 µg/mL. O ensaio temporal aponta que a partir da oitava hora o extrato hexânico tem atividade de inibição.
Palavras-chave: Inibição. Hexano. Microalgas. Bactérias Patogênicas.

Abstract
The study aimed to qualitatively evaluate the inhibitory effect of Chlorella sorokiniana extracts obtained by extraction with different organic solvents of increasing polarity on pathogenic bacteria. The microalga C. sorokiniana strain IPR 7104 was lyophilized and subjected to extractions with the following solvents: hexane, dichloromethane, ethyl acetate, and methanol for subsequent inhibitory assay. For the inhibition test, Escherichia coli, Salmonella enterica, and Serratia marcescens were cultured in Müeller-Hinton medium, and from this, a bacterial inoculum was prepared, diluted at a 1/100 ratio. 195 µL of bacterial inoculum was placed in a test tube with 5 µL of the different C. sorokiniana extracts. The tube contents were plated and evaluated. The results of the antibacterial assay with C. sorokiniana extracts obtained by extraction with different solvents indicated that only the hexane extract at a concentration of 500 µg/mL showed inhibition for the bacterium Serratia marcescens. The minimum inhibitory concentration of the hexane extract of C. sorokiniana is 400 µg/mL. The time-course assay indicates that from the eighth hour, the hexane extract exhibits inhibitory activity.
Keywords: Inhibition. Hexane. Microalgae. Pathogenic Bacteria.
Acculturation, Perceptions about Seeking Mental Health Care, and Utilization of Mental Health Services among US-based South Asians
Authors: Kapur Reet , Badsa Konya , Kapadia Farzana 
Published: October 2024

Objectives
To examine the relationship between acculturation, cultural perceptions surrounding mental health (MH) burden and utilization of MH services among South Asian (SA) adults in the United States.

Design
Cross-sectional study.

Setting
Online survey.

Participants
Four hundred five SA adults (≥18 years old) residing in the United States.

Methods
Cross-sectional study of acculturation, attitudes toward MH burden, and MH service utilization was conducted via online survey. Multivariate logistic regression models were employed to understand how these factors were associated with MH service utilization.

Main outcome measure
Utilization of MH services.

Results
Among the 405 participants, 49.0% identified as immigrants (75.1% Indian, 8% Bangladeshi, 5.5% Pakistani, and 3.8% from other SA countries). Current utilization of MH services was associated with comfort conversing in English (odds ration [OR]=5.99; 95% confidence interval [CI]=1.63, 27.02), having English-speaking peers (OR=3.80; 95% CI=1.12, 12.93), and having family (OR=2.37; 95% CI=1.21, 4.64) and peers (OR=5.64; 95% CI=1.71, 18.66) who used MH services. Participants with mostly SA peers (OR=0.48; 95% CI=0.23, 0.97) reported lower lifetime MH service utilization, and those with positive perceptions about MH burden reported higher lifetime utilization (OR=1.04; 95% CI=1.01, 1.09).

Conclusions
Comfort expressing emotions in English, knowledge of family/peer MH service use, and type of social connections were associated with MH service utilization among SA immigrants. Interventions should aim to increase SA languages in which MH services are offered and to engage with SA communities to increase acceptance of MH service utilization.
Revolutionizing Postpartum Hemorrhage Prediction with Insights from Iraq
Authors: Alshaibani Ola Abdullah , Husein Zahraa Khudair 
Published: October 2024

Background: Postpartum hemorrhage (PPH) is a severe complication affecting women with gestational hypertension or preeclampsia, yet predictive models for PPH in this population remain underdeveloped. Specific Background: Existing studies have not adequately addressed the combined influence of antepartum and intrapartum factors on the predictability of PPH. Knowledge Gap: There is a lack of comprehensive models integrating both antepartum and intrapartum variables to predict PPH risk in women with gestational hypertension or mild preeclampsia. Aims: The study investigates the predictability of preeclampsia (PPH) in women with gestational hypertension or preeclampsia at term using logistic regression models incorporating both antepartum and intrapartum factors. Methods: The study, conducted in Karbala, Iraq, involved 1252 women with hypertension or preeclampsia, developed two logistic regression models, and assessed their predictive efficacy using receiver operating characteristic analysis and calibration techniques. Results: A study found that 168 participants (10.4%) experienced preterm pregnancies, with antepartum predictors including maternal age, pre-pregnancy BMI, and preeclampsia, and intrapartum factors like gestational age and labor duration. Novelty: The study introduces a novel predictive model for Pregnancy-Positive Hypertension (PPH) that integrates antepartum and intrapartum variables for risk assessment in high-risk populations. Implications: The study suggests that combining antepartum and intrapartum variables can improve risk stratification and preventive measures, requiring further refinement for improved maternal care outcomes.
Highlights:

Antepartum and intrapartum variables enhance PPH prediction accuracy.
Model B outperforms antepartum-only model in predicting PPH.
Results stress need for improved risk stratification and prevention strategies.

Keywords: postpartum hemorrhage, gestational hypertension, preeclampsia, predictive modeling, logistic regression
Multidrug Resistant Bacteria Isolated From Some Basrah Hospitals
Authors: Dhahir Aymen Wasfi , Mohammad Noor J. 
Published: October 2024

General Background: Antibiotic resistance is a significant and growing public health concern, especially in hospital settings, where intensive care units (ICUs) often harbor multidrug-resistant organisms. Specific Background: Antimicrobial resistance in ICUs is a significant issue, necessitating a thorough assessment of bacterial susceptibility patterns to develop effective treatment protocols. Knowledge Gap: Despite the increasing concern, comprehensive studies focusing on bacterial resistance patterns in ICUs, particularly in diverse hospital settings, remain limited. Aims: The study aimed to assess the resistance patterns of bacterial isolates from blood, urine, and ICU surfaces to various antibiotics and identify the most resistant species. Results: Thirty blood, twenty urine, and fifty-six environmental samples were collected and cultured. Staphylococcus spp. exhibited 75% resistance to erythromycin, while Klebsiella spp., Pantoea spp., and E. coli showed 100% resistance to multiple antibiotics, including Ticarcillin, Piperacillin, and Cefixime. Confirmatory bacterial identification was performed using the Vitek 2 compact system, and resistance was measured across 25 antibiotics from various classes. Novelty: The study highlights the alarming 100% antibiotic resistance in various ICU-associated bacterial species, emphasizing the urgent need for revised antibiotic stewardship programs. Implications: The study underscores the importance of monitoring AMR patterns in hospitals and adjusting antibiotic therapies to combat rising resistance, particularly in ICUs, highlighting the need for robust surveillance. Highlights: 100% resistance: Klebsiella, Pantoea, E. coli resist multiple antibiotics in ICU. ICU risk: Multidrug-resistant bacteria prevalent in intensive care units. Urgent need: Update antibiotic protocols, enhance AMR surveillance in hospitals. Keywords: Antibiotic resistance, ICU, bacterial susceptibility, multidrug-resistant organisms, hospital infections
Interference Between S. Aureus and P. Aeruginosa Clinical Isolates
Authors: Najeeb Aghadeer Sarmad , Khalaf Nagham Mohamed , Kamil Israa Salim 
Published: October 2024

General Background: Understanding microbial interactions between Pseudomonas aeruginosa and Staphylococcus aureus is crucial for clinical infections, as they often coexist and influence each other's growth and virulence. Specific Background: Both organisms are known for their ability to form biofilms and exhibit multidrug resistance (MDR), complicating treatment strategies in hospitalized patients. Knowledge Gap: Previous studies have explored the virulence of P. aeruginosa and S. aureus, but there is limited understanding of their direct in vitro interactions, particularly in protease and lipase production. Aims: The study examined the interaction between P. aeruginosa and S. aureus isolates in hospitalized patients' sputum, urine, and blood samples, focusing on virulence factors, biofilm formation, and antibiotic resistance patterns. Results: In Diyala, Iraq, 50 clinical isolates showed P. aeruginosa as protease producers, lipase producers, and biofilm producers, with significant MDR phenotypes in both species. Novelty: This study highlights the ability of P. aeruginosa to produce staphylolysin, offering novel insights into the antagonistic mechanisms that may suppress S. aureus in co-infections. Implications: The study emphasizes the significance of understanding microbial interactions in clinical infections, particularly in biofilm-associated MDR infections, to improve treatment outcomes and guide more effective therapeutic approaches.
Highlights:

P. aeruginosa staphylolysin inhibits S. aureus growth in vitro.
Both bacteria produce biofilms and exhibit multidrug resistance.
Microbial interactions impact infection severity and treatment strategies.

Keywords: Microbial interactions, Pseudomonas aeruginosa, Staphylococcus aureus, Biofilm, Multidrug resistance
A Study of Diabetes Correlated Emotional Distress among Patients with Type 2 Diabetes Mellitus: A cross Sectional Study
Authors: Jassim Saja Kareem , Abbass Zahraa , Tiryag Ali Malik 
Published: October 2024

Background: Diabetes distress (DD) is a significant concern for individuals with type 2 diabetes, marked by emotional strain related to disease management and support, distinct from general psychological distress. Specific Background: Addressing DD can improve self-care practices and glycemic control, yet limited research exists on the relationship between emotional distress and sociodemographic factors in this population. Knowledge Gap: This study aims to fill this gap by exploring how sociodemographic traits influence emotional distress among type 2 diabetes patients. Aims: The primary objectives are to identify the correlation between emotional distress and diabetes and to determine the impact of sociodemographic characteristics on this distress. Results: A descriptive cross-sectional study conducted at the Endocrinology and Diabetes Center in Al-Basrah City included 150 patients aged 50–65 years, with a significant proportion of men (63.3%). The findings revealed a mean emotional distress score of 3.81, indicating high distress levels, with physician-associated distress at 3.32 and regimen-related distress at 3.48. Novelty: This research highlights the strong correlation between emotional and diabetes-related distress, particularly across different age and gender demographics, thus emphasizing the need for tailored interventions. Implications: The study emphasizes the importance of incorporating psychological assessments into diabetes management and policymakers' consideration of emotional dimensions in health program designs to improve patient self-management and quality of life.
Highlights:

Diabetes distress impacts self-care and glycemic control.
Socio-demographics influence emotional distress levels in patients.
Psychological assessments are crucial in diabetes management strategies.

Keywords: diabetes distress, emotional distress, type 2 diabetes, sociodemographic factors, healthcare interventions
Higher Serum Level of MMP-3 and Homocysteine in Patients Admitted With COVID-19
Authors: Hayawi Nasrin , Youssefi Masoud , Soukhtanloo Mohammad , Jarahi Lida , Pishbin Elham , Avval Farnaz Zahedi 
Published: October 2024

Background: SARS-CoV-2 predominantly affects the lungs, leading to severe acute respiratory syndrome (ARDS). The lack of specific biomarkers underscores the urgent need for novel indicators for early diagnosis and severity assessment of COVID-19. Specific Background: Matrix metalloproteinase-3 (MMP-3) is implicated in various inflammatory diseases, particularly viral infections, while homocysteine (Hcy) plays a crucial role in maintaining cell homeostasis and regulating inflammatory responses. Knowledge Gap: Despite their relevance in inflammation, the potential of MMP-3 and Hcy as biomarkers for COVID-19 remains underexplored. Aims: This study aimed to evaluate the serum levels of MMP-3 and Hcy in COVID-19 patients and assess their utility in diagnosis and severity prediction. Results: A study analyzing 90 serum samples from 60 ICU patients and 30 healthy controls found elevated CRP levels, higher Hcy and MMP-3 levels in the moderate group, but lower in the ICU group, with a significant correlation between MMP-3 activity and Hcy levels. Novelty: This research highlights the potential role of MMP-3 and Hcy as valuable biomarkers for COVID-19 diagnosis. Implications: While MMP-3 and homocysteine may aid in the diagnostic process, they could not be reliably used to predict severity outcomes in COVID-19 patients. Further studies are warranted to clarify the clinical implications of these biomarkers in the context of COVID-19.
Highlights:

MMP-3 and homocysteine identified as potential COVID-19 biomarkers.
No correlation found between biomarkers and disease severity.
Further research needed for clinical utility assessment.

Keywords: COVID-19, MMP-3, homocysteine, biomarkers, diagnosis
Study of Some Apoptotic Protein Expration in Human Mesenchymal Stem Cells During Toxoplasma Gondii Infection
Authors: Jasim Suhair Sameer , Mohammed Safa Yassin , Erzayek Suha Sulaiman , Hussein Rand Hatem , Jasim Ridhab Ajeel 
Published: October 2024

Background: Toxoplasma gondii (T. gondii) infection is a significant health concern, particularly during pregnancy, as it can lead to fetal harm and birth abnormalities. Specific Background: The role of apoptosis in managing T. gondii infection remains poorly understood, particularly regarding its molecular mechanisms. Knowledge Gap: The impact of T. gondii infection on apoptosis in mesenchymal stem cells (MSCs) derived from human umbilical cords has not been thoroughly studied in existing literature. Aims: This study aimed to investigate the activation of apoptosis and its regulatory mechanisms in human Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) during T. gondii infection. Results: Using non-enzymatic techniques, we isolated T. gondii from infected and aborted phase-specific placentas. Our findings demonstrated a significant increase in the expression of apoptosis-activating genes (CASP2, CASP3, Bak1) in WJ-MSCs following infection, with a marked decrease in cell viability observed within 2 to 4 hours of exposure to the parasite (P≤0.05). Novelty: This study provides novel insights into the relationship between T. gondii infection and apoptosis in WJ-MSCs, highlighting the specific gene expression changes that occur in response to infection. Implications: Research on T. gondii's apoptotic pathways is crucial for developing therapeutic strategies to mitigate pregnancy-related adverse effects and improve maternal and fetal health outcomes.
Highlights:

Increased Apoptosis: T. gondii enhances apoptosis in mesenchymal stem cells.
Cell Viability Impact: Significant decrease in WJ-MSC viability after infection.
Clinical Relevance: Insights can inform strategies to reduce fetal infection risks.

Keywords: Toxoplasma gondii, apoptosis, Wharton’s Jelly mesenchymal stem cells, pregnancy, gene expression
Nurses' Knowledge of Nonalcoholic Fatty Liver Disease: A Cross-sectional Study
Authors: Rahi Eman H. , Al-Hejaj Zahraa Mahmoud Hussain , Tiryag Ali Malik 
Published: October 2024

General Background: Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver conditions, including simple steatosis, cirrhosis, liver cancer, and non-alcoholic steatohepatitis (NASH). Specific Background: Given the increasing prevalence of NAFLD, understanding healthcare professionals' knowledge about this condition is crucial for effective patient care and management. Knowledge Gap: However, there is limited research assessing nurses' knowledge regarding NAFLD, particularly in the context of Basrah City Center Hospitals. Aims: This study aimed to evaluate nurses' knowledge of NAFLD and investigate its relationship with demographic characteristics such as gender, age, years of experience, and departmental affiliation. Results: A cross-sectional survey involving 150 nurses revealed that 57.3% possessed high levels of knowledge regarding NAFLD, while 34% had moderate knowledge. Notably, a significant correlation was found between nurses working at Al-Shifaa Hospital and their knowledge levels. The majority of participants were female (64.7%) and aged 20-30 (72%), with a predominant educational background from Nursing Institutes (39.3%) and fewer than five years of experience (56%). Novelty: This study is pioneering in assessing the knowledge of NAFLD among nurses in Basrah, highlighting specific areas of strength and weakness in their understanding. Implications: The study highlights the need for targeted educational interventions to improve nurses' understanding of NAFLD, leading to improved patient care and outcomes in liver disease management. practice.
Highlights:

Increasing NAFLD prevalence necessitates enhanced healthcare provider awareness.
Nurses showed significant knowledge levels about NAFLD.
Knowledge varies significantly by hospital affiliation.

Keywords: Non-alcoholic fatty liver disease, nurses' knowledge, Basrah City, cross-sectional study, healthcare education.
The Effect of Public Communication, Youth Participation and Social Media on Community Receptions About Covid Pandemic in Village
Authors: Indriawati Novila , Rochmaniah Ainur 
Published: October 2024

Public perception of the Covid-19 pandemic is very diverse, people view events in their environment with different responses. Especially the response regarding the Covid-19 Pandemic in the Rw.06 Village area. Kalisampurno, from the various symptoms of Covid-19, people who were exposed to Covid-19 even died due to Covid-19. Therefore Rw.06 Village, Kalisampurno formed a team to help residents of Rw.06 deal with the Covid-19 Pandemic in the Rw.06 neighborhood. Starting from the existence of public communication between RW.06 Village. Kalisampurno through their respective RT, Rw.06 Village. Kalisampurno through the covid team and also through youths who live in the Rw.06 area. In addition to using public communication, the head of Rw.06 Village. Kalisampurno also spreads information about the covid pandemic through social media, especially whatsapp. With this, information can be spread to all residents of Rw.06 Village. Kalisampurno.
This research is a type of quantitative research by choosing a location in the Rw.06 area of ​​Kalisampurno Village by distributing questionnaires through google form, totaling 110 respondents with random sampling technique. With data analyst using SPSS 20.0 . program. The results of this study indicate that the Group Communication variable (X1) partially and simultaneously has an influence on the Community Reception variable (Y) in the neighborhood area of ​​Rw.06 Village. Kalisampurno.
Overcoming E-Government Challenges through Insights from Simple Apps
Authors: Ervinda Alvina Dea , Choiriyah Ilmi Usrotin 
Published: October 2024

This study explores the development strategy for e-government preparation through Simple Applications at the Sidoarjo Regency Community and Village Empowerment Service in East Java, Indonesia. Using qualitative descriptive methods, data was collected through observation, interviews, and documentation from key personnel involved in managing the Simple Application. Findings highlight successful implementation alongside challenges such as a lack of IT expertise among staff and inadequate infrastructure for effective socialization efforts. These obstacles necessitate improvements in human resources and infrastructure to sustain and expand e-government benefits across the region, promoting enhanced governmental transparency and service delivery.
Highlights:

Successful e-government with Simple Applications in Sidoarjo Regency.
Challenges: lack of IT expertise, inadequate socialization infrastructure.
Recommendations: improve human resources, enhance infrastructure for sustainable e-government.

Keywords: e-government, Simple Application, community empowerment, infrastructure challenges, human resource development
Enhancing Performance through Work Environment, Rewards, and Employee Engagement
Authors: Ghozali Mohammad Alvin Sufianto , Abadiyah Rifdah 
Published: October 2024

This quantitative study investigates the impact of work environment and rewards on employee engagement and performance in BUMDes across Porong District. Using a sample of 104 participants, the research finds that while direct effects of work environment on employee engagement were not significant, both factors indirectly influence performance through enhanced engagement. Key findings highlight the importance of facilities and boredom alleviation in the work environment, as well as learning opportunities within rewards, in shaping performance outcomes. The study underscores the pivotal role of employee engagement, characterized by enthusiasm and support, as a mediator between these variables. The implications suggest that improving work conditions and optimizing reward systems can effectively bolster employee engagement and thereby enhance organizational performance in community-owned enterprises.
Highlights:

Quantitative study on work environment, rewards, and performance in BUMDes.
Finds indirect impact of work environment on performance through engagement.
Suggests improving conditions and rewards to boost employee engagement and performance.

Keywords: Employee Engagement, Work Environment, Rewards, Performance, BUMDes