bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Frequency Based Taste Receptors Using Bioinspired Glucose Nanobiosensor
Authors: TermehYousefi Amin , Tateno Katsumi , Bagheri Samira , Tanaka Hirofumi 
Published: May 2017

AbstractA method to fabricate a bioinspired nanobiosensor using electronic-based artificial taste receptors for glucose diagnosis is presented. Fabricated bioinspired glucose nanobiosensor designated based on an artificial taste bud including an amperometric glucose biosensor and taste bud-inspired circuits. In fact, the design of the taste bud-inspired circuits was inspired by the signal-processing mechanism of taste nerves which involves two layers. The first, known as a type II cell, detects the glucose by glucose oxidase and transduces the current signal obtained for the pulse pattern is conducted to the second layer, called type III cell, to induce synchronisation of the neural spiking activity. The oscillation results of fabricated bioinspired glucose nanobiosensor confirmed an increase in the frequency of the output pulse as a function of the glucose concentration. At high glucose concentrations, the bioinspired glucose nanobiosensor showed a pulse train of alternating short and long interpulse intervals. A computational analysis performed to validate the hypothesis, which was successfully reproduced the alternating behaviour of bioinspired glucose our nanobiosensor by increasing the output frequency and alternation of pulse intervals according to the reduction in the resistivity of the biosensor.
Identification and activation of TLR4-mediated signalling pathways by alginate-derived guluronate oligosaccharide in RAW264.7 macrophages
Authors: Fang Weishan , Bi Decheng , Zheng Ruijin , Cai Nan , Xu Hong , Zhou Rui , Lu Jun , Wan Min , Xu Xu 
Published: May 2017

AbstractAlginate, a natural acidic polysaccharide extracted from marine brown seaweeds, is composed of different blocks of β-(1, 4)-D-mannuronate (M) and its C-5 epimer α-(1, 4)-L-guluronate (G). Alginate-derived guluronate oligosaccharide (GOS) readily activates macrophages. However, to understand its role in immune responses, further studies are needed to characterize GOS transport and signalling. Our results show that GOS is recognized by and upregulates Toll-like receptor 4 (TLR4) on RAW264.7 macrophages, followed by its endocytosis via TLR4. Increased expression of TLR4 and myeloid differentiation protein 2 (MD2) results in Akt phosphorylation and subsequent activation of both nuclear factor-κB (NF-κB) and mechanistic target of rapamycin (mTOR). Moreover, GOS stimulates mitogen-activated protein kinases (MAPKs); notably, c-Jun N-terminal kinase (JNK) phosphorylation depends on TLR4 initiation. All these events contribute to the production of inflammatory mediators, either together or separately. Our findings also reveal that GOS induces cytoskeleton remodelling in RAW264.7 cells and promotes macrophage proliferation in mice ascites, both of which improve innate immunity. Conclusively, our investigation demonstrates that GOS, which is dependent on TLR4, is taken up by macrophages and stimulates TLR4/Akt/NF-κB, TLR4/Akt/mTOR and MAPK signalling pathways and exerts impressive immuno-stimulatory activity.
Intrabasin Variability of East Pacific Tropical Cyclones During ENSO Regulated by Central American Gap Winds
Authors: Fu Dan , Chang Ping , Patricola Christina M. 
Published: May 2017

AbstractHurricane Patricia in 2015 was the strongest Pacific hurricane to make landfall in Mexico. Although Patricia fortuitously spared major cities, it reminded us of the threat tropical cyclones (TCs) pose in the eastern North Pacific (ENP) and the importance of improving our understanding and prediction of ENP TCs. Patricia’s intensity and the active 2015 ENP hurricane season have been partially attributed to the strong El Niño in 2015, however there is still a lack of fundamental understanding of the relationship between El Niño-Southern Oscillation (ENSO) and ENP TCs. Here, we demonstrate that ENSO drives intrabasin variability of ENP TCs, with enhanced (reduced) TC frequency in the western portion of the ENP during El Niño (La Niña), but reduced (enhanced) TC frequency in the eastern nearshore area, where landfalling TCs preferentially form. This intrabasin difference is primarily driven by the Central American Gap Winds (CAGW), which intensify (weaken) during El Niño (La Niña), producing low-level anticyclonic (cyclonic) relative vorticity anomalies and thus an unfavorable (favorable) environment for TC genesis. These findings shed new light on the dynamics linking ENP TC activity to ENSO, and highlight the importance of improving CAGW representation in models to make skillful seasonal forecasts of ENP TCs.
Pressure induced superconductivity in the antiferromagnetic Dirac material BaMnBi2
Authors: Chen Huimin , Li Lin , Zhu Qinqing , Yang Jinhu , Chen Bin , Mao Qianhui , Du Jianhua , Wang Hangdong , Fang Minghu 
Published: May 2017

AbstractThe so-called Dirac materials such as graphene and topological insulators are a new class of matter different from conventional metals and (doped) semiconductors. Superconductivity induced by doing or applying pressure in these systems may be unconventional, or host mysterious Majorana fermions. Here, we report a successfully observation of pressure-induced superconductivity in an antiferromagnetic Dirac material BaMnBi2 with T
c
of ~4 K at 2.6 GPa. Both the higher upper critical field, μ0H
c2(0) ~ 7 Tesla, and the measured current independent of T
c
precludes that superconductivity is ascribed to the Bi impurity. The similarity in ρ
ab
(B) linear behavior at high magnetic fields measured at 2 K both at ambient pressure (non-superconductivity) and 2.6 GPa (superconductivity, but at the normal state), as well as the smooth and similar change of resistivity with pressure measured at 7 K and 300 K in zero field, suggests that there may be no structure transition occurred below 2.6 GPa, and superconductivity observed here may emerge in the same phase with Dirac fermions. Our findings imply that BaMnBi2 may provide another platform for studying SC mechanism in the system with Dirac fermions.
Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms
Authors: Colinet Hervé , Pineau Charles , Com Emmanuelle 
Published: May 2017

AbstractThe regulatory mechanisms involved in the acquisition of thermal tolerance are unknown in insects. Reversible phosphorylation is a widespread post-translational modification that can rapidly alter proteins function(s). Here, we conducted a large-scale comparative screening of phosphorylation networks in adult Drosophila flies that were cold-acclimated versus control. Using a modified SIMAC method followed by a multiple MS analysis strategy, we identified a large collection of phosphopeptides (about 1600) and phosphoproteins (about 500) in both groups, with good enrichment efficacy (80%). The saturation curves from the four biological replicates revealed that the phosphoproteome was rather well covered under our experimental conditions. Acclimation evoked a strong phosphoproteomic signal characterized by large sets of unique and differential phosphoproteins. These were involved in several major GO superclusters of which cytoskeleton organization, positive regulation of transport, cell cycle, and RNA processing were particularly enriched. Data suggest that phosphoproteomic changes in response to acclimation were mainly localized within cytoskeletal network, and particularly within microtubule associated complexes. This study opens up novel research avenues for exploring the complex regulatory networks that lead to acquired thermal tolerance.
Structure of polyacrylate/nanoparticle interfaces
Authors: Matsuura Kazuki , Matsushita Minoru , Matsuda Yasuhiro , Tasaka Shigeru 
Published: May 2017

The structure of polymer/nanoparticle interfaces was discussed using X‐ray diffraction measurements with mixtures of polyacrylates [poly(ethyl acrylate) and poly(n‐buthyl acrylate)] and nanoparticles (Al2O3 and SiO2). The amorphous halo with two peaks present for the bulk polymers changed, both in diffraction angle and intensity, into a peak in the mixtures. A novel polyacrylate structure at the interface (5–10 nm) with nanoparticles was formed with a specific chain conformation that was different from the bulk structure observed using Fourier transfer infrared spectroscopy.
Performance of Robotic Assistance for Skull Base Biopsy: A Phantom Study
Authors: Zhu Jian-Hua , Wang Jing , Wang Yong-Gui , Li Meng , Guo Yu-Xing , Liu Xiao-Jing , Guo Chuan-Bin 
Published: May 2017

Objectives This study aims to evaluate the feasibility of a custom robot system guided by optical cone beam computed tomography (CBCT)-based navigation for skull base biopsy.
Design An accuracy study was conducted.
Setting Platform for navigation and robot-aided surgery technology.
Participants Phantom skull.
Main Outcome Measures The primary outcome measure was to investigate the accuracy of robot-assisted needle biopsy for skull base tumors. A 14-gauge needle was automatically inserted by the five degrees of freedom robot into the intended target, guided by optical navigation. The result was displayed on the graphical user interface after matrix transformation. Postoperative image scanning was performed, and the result was verified with image fusion.
Results All 20 interventions were successfully performed. The mean deviation of the needle tip was 0.56 ± 0.22 mm (measured by the navigation system) versus 1.73 ± 0.60 mm (measured by image fusion) (p < 0.05). The mean insertion depth was 52.3 mm (range: 49.7–55.2 mm). The mean angular deviations off the x-axis, y-axis, and z-axis were 1.51 ± 0.67, 2.33 ± 1.65, and 1.47 ± 1.16 degrees, respectively.
Conclusions The experimental results show the robot system is efficient, reliable, and safe. The navigation accuracy is a significant factor in robotic procedures.
Management des massiven intraoperativen Blutverlusts anhand eines Fallbeispiels
Authors: Pape Andreas , Ippolito Angelo , Warszawska Joanna , Raimann Florian J. , Zacharowski Kai 
Published: May 2017

ZusammenfassungDer massive intraoperative Blutverlust stellt eine potenziell lebensbedrohliche Komplikation während eines operativen Eingriffs dar. Die letale Trias aus schockbedingter Azidose, Hypothermie und Koagulopathie verstärkt die Blutungsneigung zusätzlich. Um diesen circulus vitiosus zu vermeiden, erfordert das Management einer intraoperativen Massivblutung ein strukturiertes und standardisiertes Vorgehen. Vorrangige Behandlungsziele sind dabei die Aufrechterhaltung einer adäquaten Gewebeoxygenierung, die Wiederherstellung einer suffizienten Gerinnungsfunktion, sowie Normothermie und die Homöostase des Säure-Basen- und Elektrolythaushaltes. Der vorliegende Artikel veranschaulicht diese Therapieziele und ihre pathophysiologischen Hintergründe anhand eines Fallbeispiels.
Participation after Stroke and Traumatic Brain Injury: Results of a Pilot Study in Outpatient Neurorehabilitation in Vorarlberg
Authors: Pöppl Dominik , Marheineke Judith , Deck Ruth 
Published: May 2017

Abstract
Objective A multicentric study has reported on the sustained effects of outpatient neurorehabilitation in Germany. Different to Germany, the biopsychosocial model of disability, as described in the International Classification of Functioning, Disability and Health (published by the World Health Organization), is not incorporated into Austrian law. There is a need for objective measurement instruments for routine outcome evaluation of outpatient neurorehabilitation in Austria. The present examines the extent to which the instruments used for the German multicentric study are applicable in the Austrian rehabilitation context and if there are similar rehabilitation effects over time.
Method In a multicentric observational study, patients of 4 Austrian outpatient neurorehabilitation centers for stroke or traumatic brain injury were asked to fill in a questionnaire. This was carried out 4 times throughout the program: on admission and discharge as well as on month 4 and 12 post rehabilitation. Additionally, clinical experts were asked to rate the patients’ status at admission and discharge.
Results and conclusions The results of 4 outpatient neurorehabilitation centers in Vorarlberg (Austria) suggest that the used measurement instruments are reasonable for the evaluation of outpatient neurorehabilitation in Austria, based on the ultimate goal “participation and self-determined conduct of life”. For routine outcome evaluation, fewer instruments should be used and adjustments should be made while assessing more severely affected patients.
Frei fluktuierende intraokulare Zysten
Authors: Werner Jens , Lang Gerhard , Enders Christian 
Published: May 2017

Zusammenfassung
Hintergrund Frei fluktuierende intraokulare Zysten lassen sich in der Vorderkammer (FZV) und im Glaskörper (FZG) finden. Vor fast 150 Jahren wurde erstmals eine solche frei fluktuierende Zyste beschrieben; sie gelten als okuläre Raritäten.
Material und Methoden Es werden exemplarisch 2 Patienten demonstriert. Anhand dieser Beispiele erfolgt eine Übersicht über den aktuellen Wissensstand.
Ergebnisse und Schlussfolgerung Patient 1 zeigte als Zufallsbefund eine FZV, die glatt begrenzt, transluzide und leicht pigmentiert war. Die Ultraschallbiomikroskopie zeigte einen echofreien Binnenraum der Zyste auf. Bei Beschwerdefreiheit und fehlender Behandlungsindikation wurden lediglich Verlaufskontrollen angeraten. Zysten der Iris lassen sich in primäre und sekundäre einteilen. Primäre Zysten der Iris können sowohl vom Stroma als auch vom Pigmentepithel abstammen, wobei FZV i. d. R. vom Pigmentepithel entstehen. Sekundäre Zysten und auch FZV können durch Tumoren, Inflammation, Epithelimplantation, Ophthalmologikaanwendung oder aber intraokulare Fremdkörper induziert werden. Patientin 2 wies neben deutlichen myopen Fundusveränderungen eine FZG mit gelb-grünlicher Oberfläche auf; die Transparenz war reduziert und die Oberfläche nicht pigmentiert. Auch hier zeigte die Sonografie einen echofreien Binnenraum und es wurden lediglich Verlaufskontrollen angeraten. Bei FZG werden angeborene und erworbene Ursachen diskutiert. So könnten FZG ebenfalls vom Pigmentepithel der Iris abstammen, es gibt jedoch diesbezüglich widersprüchliche Untersuchungsergebnisse. Als erworbene Ursachen werden Trauma, Inflammation und chorioretinale Erkrankungen in Betracht gezogen. Vor allem bei FZG ist die Genese nach wie vor nicht abschließend geklärt. Wichtig für die Behandlung von Patienten mit FZV und FZG ist eine genaue Kenntnis über mögliche Entstehungsursachen, um eine Therapieentscheidung treffen zu können. Bei Verlaufskontrollen ist eine qualitativ hochwertige Dokumentation mit Fotografie und Ultraschall (-biomikroskopie) erforderlich.
Impact of backbone fluorination on nanoscale morphology and excitonic coupling in polythiophenes
Authors: Hu Zhongjian , Haws Ryan T. , Fei Zhuping , Boufflet Pierre , Heeney Martin , Rossky Peter J. , Vanden Bout David A. 
Published: May 2017

Significance
Conjugated polymers (CPs) are some of the most attractive organic semiconductors for flexible optoelectronic device applications. The structural and optoelectronic properties of CPs are dependent on both the conformation of individual polymer chains and interactions between chains. Chemists have sought to control these interactions by creating more planar polymers that can pack tightly together and share strong electronic interactions. Single-molecule spectroscopy (SMS) studies of a fluorinated CP show that backbone fluorination does, in fact, lead to more planar polymer chains and highly ordered aggregates. Interestingly though, the electronic interactions are found to extend along individual chains rather than between chains. The studies demonstrate how SMS can test hypotheses about structure–property relationships in large molecular systems.
La alternancia de código como variable estratégica de decisiones económicas en la industria musical
Authors: Jiménez Palmero Diego , Jiménez Palmero Alfredo 
Published: May 2017

Resumen
En este trabajo se analiza el impacto de la alternancia de código o code-switching en el éxito musical de las canciones recientemente lanzadas al mercado. Tras efectuar una revisión de la literatura sobre el bilingüismo en general y la alternancia de código en particular, se analiza una muestra de 284 canciones a través de un análisis de corpus así como de un enfoque empírico y cuantitativo basado en la regresión binomial negativa y la minimocuadrática. Los resultados obtenidos demuestran que el empleo de la alternancia de código tiene un impacto positivo y significativo sobre el éxito de la canción medido a través de diversos indicadores, lo que verifica y refuerza la necesidad de tener en consideración las importantes repercusiones, en este caso de naturaleza socioeconómica, que la lingüística ejerce en el mundo actual.
Estudio longitudinal del desarrollo del acento en español como primera lengua
Authors: Polo Nuria 
Published: May 2017

Resumen
El presente trabajo propone un análisis de la adquisición del acento basado en el desarrollo de la estructura prosódica del español durante los primeros meses del desarrollo lingüístico. Hay muy poca investigación sobre la adquisición del acento y el desarrollo de la estructura prosódica en esta lengua. Un repaso de la bibliografía sobre adquisición muestra dos hipótesis de trabajo: un sesgo hacia el troqueo, que interpreta que las primeras emisiones infantiles se realizan bajo una forma trocaica por defecto, y una hipótesis neutra, determinada por la estructura correspondiente de la lengua meta. Dado que no hay consenso sobre la adquisición del acento en español, se presenta este trabajo con el propósito de profundizar en el desarrollo del acento en español como primera lengua. Para ello se han analizado las producciones de dos niños monolingües hablantes de español (desde 1;7 a 2;3 años), más los datos adicionales de otro niño a 1;10 años. El estudio no ha proporcionado datos convincentes para apoyar la hipótesis trocaica en el desarrollo del acento en español; sin embargo, los niños que adquieren español dominan los patrones acentuales de esta lengua desde el principio de sus emisiones.
Effects of Short–Term Medical Mission Trips to Chiapas, Mexico, on the Religiosity of the Missionaries
Authors: Meidl Katherine A. , Meidl Joseph M. , Meidl Laura R. , Meidl Erik J. 
Published: May 2017

This study evaluates the effects that short-term, foreign, Catholic medical mission trips had on the religiosity of the United States-based participants. The subjects of this study participated in Catholic medical missions to Chiapas State, Mexico, in 2014 and 2015. Twenty-two of forty-two participants responded to a survey to assess for any changes in their religiosity and associated attitudes and behaviors. The results revealed that participation in the medical mission was associated with a significant increase in non-organizational religious activity, intrinsic religiosity, concern for health disparities and the burden of illness in the developing world, the promotion of further missions, the provision of service and/or monetary aid to the poor in the missionary's local community, and an increased likelihood to discuss the Christian faith in conversations with others. There was no statistically significant association with organizational religious activity or local participation in evangelization activities. Summary This article reports on the changes seen in the religious attitudes and charitable works performed following participation in a short-term medical mission. After serving on a mission trip to Mexico, we found that United States-based missionaries had an increase in their private religious activities, felt closer to God, were more likely to help the poor in their own neighborhoods, and were more likely to discuss their Christian faith than prior to the mission trip. We discuss possible reasons for these changes.
Taking Down Barcelona in Kathmandu: Linguistic Futures in a Student Movement
Authors: Weinberg Miranda 
Published: May 2017

AbstractIn June 2012, student activists in Nepal declared a campaign against private, for-profit colleges with foreign names, simultaneously decrying the schools’ names and their exorbitant tuition fees. During the campaign, members of multiple student unions vandalized signboards, buildings, computers, and buses belonging to various colleges and filed a court case demanding stricter management of private schools. These activists claimed control of the linguistic landscape of Kathmandu, objecting not to English in the classroom but to the material emblems of branded educational institutions. This article explores the semiotic implications of this movement through analysis of newspaper coverage of the protests. The school names and talk about appropriate names delineate two competing cultural chronotopes that students employed to promote a particular vision of proper Nepali behavior and to contest what they depicted as inappropriate commodification of higher education.
Reliability of algorithmic somatic copy number alteration detection from targeted capture data
Authors: Rieber Nora , Bohnert Regina , Ziehm Ulrike , Jansen Gunther 
Published: May 2017

Abstract

Motivation
Whole exome and gene panel sequencing are increasingly used for oncological diagnostics. To investigate the accuracy of SCNA detection algorithms on simulated and clinical tumor samples, the precision and sensitivity of four SCNA callers were measured using 50 simulated whole exome and 50 simulated targeted gene panel datasets, and using 119 TCGA tumor samples for which SNP array data were available.

Results
On synthetic exome and panel data, VarScan2 mostly called false positives, whereas Control-FREEC was precise (&gt;90% correct calls) at the cost of low sensitivity (&lt;40% detected). ONCOCNV was slightly less precise on gene panel data, with similarly low sensitivity. This could be explained by low sensitivity for amplifications and high precision for deletions. Surprisingly, these results were not strongly affected by moderate tumor impurities; only contaminations with more than 60% non-cancerous cells resulted in strongly declining precision and sensitivity. On the 119 clinical samples, both Control-FREEC and CNVkit called 71.8% and 94%, respectively, of the SCNAs found by the SNP arrays, but with a considerable amount of false positives (precision 29% and 4.9%).

Discussion
Whole exome and targeted gene panel methods by design limit the precision of SCNA callers, making them prone to false positives. SCNA calls cannot easily be integrated in clinical pipelines that use data from targeted capture-based sequencing. If used at all, they need to be cross-validated using orthogonal methods.

Availability and implementation
Scripts are provided as supplementary information.

Supplementary information
Supplementary data are available at Bioinformatics online.