bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploiting Drug Addiction Mechanisms to Select against MAPKi-Resistant Melanoma
Authors: Hong Aayoung , Moriceau Gatien , Sun Lu , Lomeli Shirley , Piva Marco , Damoiseaux Robert , Holmen Sheri L. , Sharpless Norman E. , Hugo Willy , Lo Roger S. 
Published: September 2017

Abstract
Melanoma resistant to MAPK inhibitors (MAPKi) displays loss of fitness upon experimental MAPKi withdrawal and, clinically, may be resensitized to MAPKi therapy after a drug holiday. Here, we uncovered and therapeutically exploited the mechanisms of MAPKi addiction in MAPKi-resistant BRAFMUT or NRASMUT melanoma. MAPKi-addiction phenotypes evident upon drug withdrawal spanned transient cell-cycle slowdown to cell-death responses, the latter of which required a robust phosphorylated ERK (pERK) rebound. Generally, drug withdrawal–induced pERK rebound upregulated p38–FRA1–JUNB–CDKN1A and downregulated proliferation, but only a robust pERK rebound resulted in DNA damage and parthanatos-related cell death. Importantly, pharmacologically impairing DNA damage repair during MAPKi withdrawal augmented MAPKi addiction across the board by converting a cell-cycle deceleration to a caspase-dependent cell-death response or by furthering parthanatos-related cell death. Specifically in MEKi-resistant NRASMUT or atypical BRAFMUT melanoma, treatment with a type I RAF inhibitor intensified pERK rebound elicited by MEKi withdrawal, thereby promoting a cell death–predominant MAPKi-addiction phenotype. Thus, MAPKi discontinuation upon disease progression should be coupled with specific strategies that augment MAPKi addiction.
Significance: Discontinuing targeted therapy may select against drug-resistant tumor clones, but drug-addiction mechanisms are ill-defined. Using melanoma resistant to but withdrawn from MAPKi, we defined a synthetic lethality between supraphysiologic levels of pERK and DNA damage. Actively promoting this synthetic lethality could rationalize sequential/rotational regimens that address evolving vulnerabilities. Cancer Discov; 8(1); 74–93. ©2017 AACR.
See related commentary by Stern, p. 20.
This article is highlighted in the In This Issue feature, p. 1
Bridging Operational Meteorology and Academia through Experiential Education: The Storm Prediction Center in the University of Oklahoma Classroom
Authors: Cohen Ariel E. , Thompson Richard L. , Cavallo Steven M. , Edwards Roger , Weiss Steven J. , Hart John A. , Jirak Israel L. , Bunting William F. , Rogers Jaret W. , Piltz Steven F. , Gerard Alan E. , Moore Andrew D. , Cornish Daniel J. , Boothe Alexander C. , Cohen Joel B. 
Published: September 2017

Abstract
During the 2014–15 academic year, the National Oceanic and Atmospheric Administration (NOAA) National Weather Service Storm Prediction Center (SPC) and the University of Oklahoma (OU) School of Meteorology jointly created the first SPC-led course at OU focused on connecting traditional theory taught in the academic curriculum with operational meteorology. This class, “Applications of Meteorological Theory to Severe-Thunderstorm Forecasting,” began in 2015. From 2015 through 2017, this spring–semester course has engaged 56 students in theoretical skills and related hands-on weather analysis and forecasting applications, taught by over a dozen meteorologists from the SPC, the NOAA National Severe Storms Laboratory, and the NOAA National Weather Service Forecast Offices. Following introductory material, which addresses many theoretical principles relevant to operational meteorology, numerous presentations and hands-on activities focused on instructors’ areas of expertise are provided to students. Topics include the following: storm-induced perturbation pressure gradients and their enhancement to supercells, tornadogenesis, tropical cyclone tornadoes, severe wind forecasting, surface and upper-air analyses and their interpretation, and forecast decision-making. This collaborative approach has strengthened bonds between meteorologists in operations, research, and academia, while introducing OU meteorology students to the vast array of severe thunderstorm forecast challenges, state-of-the-art operational and research tools, communication of high-impact weather information, and teamwork skills. The methods of collaborative instruction and experiential education have been found to strengthen both operational–academic relationships and students’ appreciation of the intricacies of severe thunderstorm forecasting, as detailed in this article.
Comparison of stored red blood cell washing techniques for priming extracorporeal circuits
Authors: Sasaki Jun , Tirotta Christopher , Lim Hyunsoo , Kubes Kathleen , Salvaggio Jane , Hannan Robert , Burke Redmond , Ojito Jorge 
Published: September 2017

Background: The aim of this study was to compare three different blood washing techniques and describe the differences for the composition of the washed red blood cells (RBC). Methods: Stored RBCs less than 5 days old were washed using three different techniques. 1) Washing with normal saline with the COBE Model 2991 blood processor in the blood bank (BB-S). 2) Washing with normal saline with the Continuous AutoTransfusion System (C.A.T.S) in the operating room (OR-S). 3) Washing with Plasma-Lyte with the C.A.T.S in the operating room (OR-PL). Then, we compared the values for hemoglobin (Hb), hematocrit (Hct), blood volume, RBC volume, lactate, glucose, sodium and potassium of the three different groups. Results: Forty-five units of RBCs were washed and analyzed (15 for each technique). The OR-S RBCs, when compared to the BB-S RBCs, had lower hemoglobin (g/dL) (22.8 vs 24.1, p=0.006), lower hematocrit (%) (67 vs 71, p=0.006), higher RBC volume (ml) (161 vs 130, p<0.001), higher glucose (mg/dL) (185 vs 46, p<0.001) and lower sodium (mmol/L) (153 vs 158, p<0.001). When compared to the OR-S RBCs, the OR-PL RBCs showed higher potassium (mmol/L) (5.3 vs 2, p<0.001) and lower sodium (mmol/L) (129 vs 153, p<0.001). Conclusion: RBCs washed with an autotransfusion device had a higher RBC volume and more physiological levels of glucose and sodium when compared with the blood processor in the blood bank. It can be an alternative option to use RBCs washed with an autotransfusion device for priming the extracorporeal circuits utilized in patients undergoing cardiac surgery.
Cultural Identity in the Age of Transculturation
Authors: Um Jungsik 
Published: September 2017

AbstractThe term “self” refers to the subject which persists through the changing experiences of a person. We think of it at least as the center of personal identity, or the organizing function within the individual. It may be desirable to examine cultural identity if there is something like “the communal self.” Kant believed that there is a “transcendental self” which transcends our experiences. Following him, we may analogously claim that there is a communal self, which transcends the history and the culture of the community, maintaining their identity throughout time by means of providing the unity principle. Korea is a typical example of a nation whose process of transculturation is most dynamically ongoing. Such a nation should apply this concept to her current cultural situations in order to be successful in the age of globalization.
Depression and Cognitive Function in Mild Cognitive Impairment: A 1-Year Follow-Up Study
Authors: Yoon Seoyoung , Shin Cheolmin , Han Changsu 
Published: September 2017

Background: The coexistence of depression with mild cognitive impairment (MCI) seems to increase the risk of dementia. However, the explanations of that relationship have been inconsistent. We investigated cognitive profiles in patients with MCI with and without depression and whether changes in depression symptoms affect cognition longitudinally. Methods: For the study, 161 patients with MCI were divided into a depressed group (D+) and a nondepressed group (D−). After 1 year, we redivided the original D− group into D− and newly developed depression (Dd) groups and the D+ group into improved depression (Di) and nonimproved depression (Dn) groups. Neuropsychological tests assessing depression and cognitive domains were performed at baseline and follow-up. Results: When age-adjusted, the D+ group showed significantly poorer performance in general cognition and some subtests regarding memory, executive function, and attention. At the 1-year follow-up, changes in the calculation test ( P = .005) and Controlled Oral Word Test (COWAT; P = .048) were significantly different between groups. Only the Di group showed significant improvement in calculation. The Dn group showed significant decrement in COWAT that was significantly different from that of the Di group, which showed no significant change. Discussion: Patients with depression having MCI showed poorer cognitive function than nondepressed patients with MCI in some cognitive domains. Improvement in depression was related to improvement or prevention of decline in cognitive measures.
Clinical and Dermoscopic Features of Pigmented Disseminated Superficial Actinic Porokeratosis: Case Report and Literature Review
Authors: Sotoodian Bahman , Mahmood Muhammad N. , Salopek Thomas G. 
Published: September 2017

Introduction: Porokeratosis is a benign hyperkeratotic skin tumour due to a clonal proliferation of keratinocytes and is characterised by a telltale annular threadlike configuration along the border of a skin-colored to erythematous papule that can expand centrifugally. Case Presentation: We are presenting a clinical and dermoscopic case of pigmented disseminated superficial actinic porokeratosis (DSAP) limited to the upper trunk of a white man with sun-damaged skin. Literature Review and Conclusion: A thorough review of PubMed failed to identify any previous reports on the dermoscopic appearance of pigmented porokeratosis. On dermoscopy, the presence of black dots limited to the periphery of the lesions is due to pigment incontinence and melanophages within the superficial papillary dermis limited to the area below the cornoid lamella. Pigmented DSAP is a unique morphological presentation of porokeratosis, and it is essential to be familiar with its clinical and dermoscopic presentation.
Ultrasound-guided radiofrequency ablation of genicular nerves of knee for relief of intractable pain from knee osteoarthritis: a case series
Authors: Ahmed Arif , Arora Divesh 
Published: September 2017

Introduction: The knee arthroplasty is the best option for patients with advanced osteoarthritis who have failed all other conservative options, but regrettably many patients fail to undergo surgery due to co-morbidities or other reasons. So, new alternative modes are always in demand for these patients. Methods: The ultrasound-guided radiofrequency ablation (RFA) of all the genicular nerves of knee joint was done in patients with grade III and IV osteoarthritis of knee joint, with severe pain (numerical rating scale (NRS) > 7) who had failed conservative management and intra-articular injections after a positive genicular nerve block with local anaesthetics. The demographics, pain intensity measured in NRS, Oxford Knee Score (OKS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and quality of life measured by 36-Item Short Form Health Survey (SF-36) questionnaire was measured at baseline and at regular intervals. Results: The average age of the patients was 61.50 ± 6.75 years. There was significant improvement in pain intensity at rest, movement and on weight bearing from 8.75 ± 0.5, 9.0 ± 0.0, and 9.0 ± 0.0 at baseline to 2.38 ± 0.51, 3.75 ± 0.46, and 4.13 ± 0.35 at 1 month and 3.13 ± 0.64, 4.38 ± 0.51, and 4.63 ± 0.51 at 6 months after the procedure, respectively (p value <0.05). The OKS had improved from 7.75 ± 1.25 at baseline to 28.88 ± 2.53 and 28.13 ± 1.80 at 1 and 6 months, respectively, after the procedure (p value <0.05). The WOMAC score had also improved significantly from 77.75 ± 4.34 at baseline to 38.38 ± 5.82 and 39.25 ± 5.12 at 1 and 6 months, respectively (p value <0.05). There was also significant improvement in the quality of life after the procedure (p value <0.05). Conclusion: Ultrasound-guided RFA of genicular nerves of knee joint is a good alternative option for patients who are having severe pain and disability from knee osteoarthritis and gives a long-lasting pain relief for more than 6 months.
Single-pedicle hinge flap performed by shelter medicine team resolves chronic antebrachial wound in a cat
Authors: Richardson Jodi M , Shivley Jacob M , Bushby Philip A 
Published: September 2017

Case summary An approximately 3-year-old, male domestic longhair cat was presented to a mobile veterinary unit for routine neuter. Preoperative physical examination revealed an approximately 5 cm × 2 cm scab on the craniolateral portion of the left antebrachium. The cat was anesthetized for the neuter using an injectable anesthesia protocol. After castration, the wound area on the antebrachium was clipped, copiously lavaged and the wound edges were surgically debrided. Injectable antibiotics and analgesic management were instituted. The wound was conservatively managed using sugar bandaging and antibiotic dressings until the progression of healing plateaued. Procedures for closing the defect were explored, and it was decided that a single-pedicle hinge flap would be ideal. The procedure was performed on the mobile veterinary unit and managed postoperatively with pain control and biweekly bandage changes. After 3 weeks, the single-pedicle hinge flap was released to create a skin graft, which successfully filled the defect. Relevance and novel information Single-pedicle hinge flaps performed in feline patients have been minimally reported. This case report serves to provide detailed information on the surgical procedure and aftercare required for a successful outcome. Furthermore, this procedure was performed by a shelter medicine team in a mobile veterinary unit with no specialty equipment or instruments. This report documents an alternative procedure that may be used in a shelter environment for distal forelimb wounds rather than amputation or euthanasia.
CK2β regulates thrombopoiesis and Ca2+-triggered platelet activation in arterial thrombosis
Authors: Münzer Patrick , Walker-Allgaier Britta , Geue Sascha , Langhauser Friederike , Geuss Eva , Stegner David , Aurbach Katja , Semeniak Daniela , Chatterjee Madhumita , Gonzalez Menendez Irene , Märklin Melanie , Quintanilla-Martinez Leticia , Salih Helmut R. , Litchfield David W. , Buchou Thierry , Kleinschnitz Christoph , Lang Florian , Nieswandt Bernhard , Pleines Irina , Schulze Harald , Gawaz Meinrad , Borst Oliver 
Published: September 2017

Key Points
CK2β is critically required for thrombopoiesis by regulating tubulin polymerization, MK fragmentation, and proplatelet formation. CK2β facilitates inositol triphosphate–mediated increase of cytosolic Ca2+ and is essential for platelet activation in arterial thrombosis in vivo.
Pain control in thoracic oncology
Authors: Peeters-Asdourian Christine , Massard Gilbert , Rana Paragi H. , Van Houtte Paul , White Andrew P. , Grigoriu Bogdan , Lossignol Dominique , Almalki Mohammed , Alexiou Jean , Engelholm Jean-Luc , Sculier Jean-Paul 
Published: September 2017

This review of pain management in lung cancer is based on the presentation of four cases of thoracic oncology patients with pain at various stages of their disease. The approach will be multidisciplinary, involving a thoracic oncologist, radiologist, thoracic and orthopaedic spine surgeon, radiation therapist, pain medicine specialist, and palliative care specialist. This multispecialty approach to the management of different painful presentations in thoracic oncology will demonstrate the complexity of each case and the improved patient outcomes which result from the involvement of different disciplines working in concert.In the USA, Europe and other countries, palliative care specialists often become rapidly involved in the management of these patients, coordinating social care and providing psychological support.Thoracic and orthopaedic spine subspecialists provide surgical methods to control tumour invasion, and improve quality of life and preservation of function in settings of even diffuse metastatic disease. Similarly, thoracic oncology and radiation therapists utilise both therapeutic and palliative chemotherapeutic and radiation therapy regimens to prolong and improve quality of life.The pain medicine specialist can, in addition to medication management, offer a variety of interventional approaches including unique drug delivery systems such as epidural analgesia, regional anaesthesia techniques, and intrathecal pumps, as well as neuromodulation techniques and neurolytic or neuroablative procedures.In the USA, these specialists complete an additional fellowship year in pain medicine following the completion of an anaesthesiology, physical medicine and rehabilitation, neurology or psychiatry residency. These programmes are accredited by the Accreditation Council for Graduate Medical Education, or ACGME (www.acgme.org).
Case study on 3D printing education in fashion design coursework
Authors: Kwon Yu Mi , Lee Young-A , Kim Sook Jin 
Published: September 2017

AbstractIn the past 10 years, the use of 3D printing (3DP) has been increased in a number of industries. However, its use in the fashion industry and education is still at the early stage. This study aimed to provide (1) a general overview of 3DP in fashion and its integration in fashion education and (2) a pedagogical guidance to educators in the fashion discipline by presenting one case of 3D printing education in fashion design courses. This case study focused on the importance of 3D printing in fashion design curricula and the way to strategically integrate this technology into the curriculum. Rhinoceros (Rhino) and FINEBOT were used for 3D object design and printing, respectively. The coursework consisted of theoretical lectures including definition and application of 3D printing; training of Rhino use; methods of producing objects; 3D modeling of creative objects; and 3D printing practice. During this coursework, the course instructor allowed for students to initiate their design and create the 3D objects on what they wanted. Challenges for educators were identified when fashion students were learning 3D modeling software; however, the coursework resulted in increased interest and understanding of the students in 3D modeling and printing of more advanced fashion-related objects.
Illness perceptions in adolescents with a psychiatric diagnosis in Pakistan
Authors: Imran Nazish , Azeem Muhammad Waqar , Chaudhry Mansoor R. , Butt Zeeshan 
Published: September 2017

Aims and methodTo assess adolescents' perceptions of their psychiatric illness and the role of various demographic factors in a Pakistani setting. Adolescents with various psychiatric diagnoses were interviewed using a structured questionnaire including the Illness Perceptions Questionnaire–Revised (IPQ-R).ResultsFifty-two adolescents with various psychiatric illnesses were interviewed; their mean age was 12.7 years and the majority (67%) were female. Males had significantly higher scores on timeline and emotional representation (P<0.05), suggesting strongly held beliefs about chronicity of their illness and anger and worry about their condition. Adolescents' own emotional state, stress, family problems and bad luck were endorsed by participants as some of the causal factors in their mental illness.Clinical implicationsDespite the importance of early intervention in psychiatric problems, engaging youth in the treatment process in Pakistan remains difficult. Better understanding of how adolescents perceive their psychiatric difficulties may play a significant role in developing culturally sensitive interventions and better utilisation of services.
‘Spirituality’ and ‘cultural adaptation’ in a Latino mutual aid group for substance misuse and mental health
Authors: Anderson Brian T. , Garcia Angela 
Published: September 2017

SummaryA previously unknown Spanish-language mutual aid resource for substance use and mental health concerns is available in Latino communities across the USA and much of Latin America. This kind of ‘4th and 5th step’ group is a ‘culturally adapted’ version of the 12-step programme and provides empirical grounds on which to re-theorise the importance of spirituality and culture in mutual aid recovery groups. This article presents ethnographic data on this organisation.
Effectiveness of model-based clustering in analyzing Plasmodium falciparum RNA-seq time-course data
Authors: Oyelade Jelili , Isewon Itunuoluwa , Olaniyan Damilare , Rotimi Solomon O , Soyemi Jumoke 
Published: September 2017

Background: The genomics and microarray technology played tremendous roles in the amount of biologically useful information on gene expression of thousands of genes to be simultaneously observed. This required various computational methods of analyzing these amounts of data in order to discover information about gene function and regulatory mechanisms. Methods: In this research, we investigated the usefulness of hidden markov models (HMM) as a method of clustering Plasmodium falciparum genes that show similar expression patterns. The Baum-Welch algorithm was used to train the dataset to determine the maximum likelihood estimate of the Model parameters. Cluster validation was conducted by performing a likelihood ratio test. Results: The fitted HMM was able to identify 3 clusters from the dataset and sixteen functional enrichment in the cluster set were found. This method efficiently clustered the genes based on their expression pattern while identifying erythrocyte membrane protein 1 as a prominent and diverse protein in P. falciparum. Conclusion: The ability of HMM to identify 3 clusters with sixteen functional enrichment from the 2000 genes makes this a useful method in functional cluster analysis for P. falciparum
Collapse characteristics of a circular-cross-section CFRP pipe structure member using finite element analysis
Authors: Kim Dae Young , Kim Hee Seong , Kim Ji Hoon 
Published: September 2017

AbstractCarbon fiber reinforced plastics (CFRPs) are advanced composite materials that have been used as lightweight structural materials for vehicles. Unlike general isotropic materials, the structural characteristics of composite materials are strongly influenced by the stacking directions and sequences of the composite laminates. In this study, finite element analysis was used to predict the material properties of the carbon fibers and the resin composing a CFRP in cases of laminated carbon fibers and modified external angles. The results verify the approach’s reliability by comparing the simulation results and the real test results related to the material properties of the carbon fibers and the resin. The results of the finite element analysis and the experimental results were compared with the load-displacement curves and the maximum load. The [02/902]S, [902/02]2, and [0/90]2S specimens showed a maximum error rate of 8.6%, whereas the [902/02]S, [02/902]2, and [90/0]2S specimens showed a maximum error rate of approximately 12.93%. By applying CFRP static collapse analysis of fiber properties and resin properties through basic experiments and basic theory, we predicted the properties of CFRPs through finite element analysis; an error rate of approximately 10% indicated that our approach is effective.
Maternal plasma LPCAT 1 mRNA correlates with lamellar body count
Authors: Welch Robert A. , Recanati Maurice-Andre , Welch Kathryn C. , Shaw Michael K. 
Published: September 2017

AbstractHuman lysophosphatidylcholine acyltransferase 1 (hLPCAT1) is a protein which helps produce surfactant in the fetal lung. We previously reported that levels of cell-free fetal mRNA for hLPCAT1 in amniotic fluid are correlated with lamellar body count (LBC) (r2=0.93). This short communication demonstrates that fetal hLPCAT1 mRNA is also present in maternal blood. Its quantity also correlates with amniotic fluid LBC (r2=0.81). Research in maternal plasma hLPCAT1 may assist in understanding fetal and placental maturational processes.