bluuebunny/crossref_metadata_2025_split huggingface dataset:
EGF is highly expressed in hepatocellular carcinoma (HCC) and promotes motility of HCC cells via fibronectin
Authors: Liu Zongcai , Chen Danyang , Ning Fen , Du Jun , Wang Haifang 
Published: January 2018

AbstractBetter understanding of metastasis process would allow for the development of effective approaches to treat hepatocellular carcinoma (HCC). Recent literature has highlighted the fundamental role of interaction between tumor cells and their microenvironment components in tumor metastasis. Aberrant expression of epidermal growth factor (EGF) induces highly malignant HCC, and activated EGF/EGFR signaling is correlated with an aggressive phenotype and intrahepatic metastasis. Thus, EGF in the tumor microenvironment may influence the behavior of HCC cells. In this study, for the first time, we studied the expression of EGF in HCCs, and the potential role of EGF in the motility of HCC cells and the underlying mechanisms. It was demonstrated that EGF was highly expressed in HCCs and positively associated with higher tumor grade. In addition, EGF promoted the migration and invasion of HCC cells mainly via induction of fibronectin (FN) in vitro. Mechanistically, EGF simultaneously increased the nuclear translocation and PKC mediated phosphorylation of p65 which could bind to the −356 bp to −259 bp fragment of FN promoter, leading to a markedly increased activity of FN promoter in HCC cells. These results highlight the potential role of EGF in promoting HCC metastasis, demonstrate a novel pathway for regulation of FN expression and provide potential targets for HCC prevention and treatment.
Retracted: MicroRNA‐494 regulates Gli3 expression and inhibits pancreatic cancer cells growth and migration
Authors: Ma Yongbiao , Li Gaoxue , Hu Jingxia , Liu Xin , Shi Baomin 
Published: January 2018

AbstractMicroRNA (miR)‐494 has been identified as a predictor and inhibitor in pancreatic cancer. This study aimed to explore the role of miR‐494 in pancreatic cancer cells, and the regulation of glioma‐associated oncogene 3 (Gli3) by miR‐494. The mRNA level of Gli3 in 99 pairs of pancreatic cancer and correspondingly adjacent tissues was monitored by qRT‐PCR. Correlation of Gli3 expression with miR‐494 level was assessed by Pearson χ2 test. Dual‐luciferase reporter assay was used to detect whether Gli3 was a target of miR‐494. Following miR‐494 mimics and miR‐494 inhibitor transfection, the changes in cell viability and migration were detected by using CCK‐8 and Transwell chamber. Furthermore, Gli3 siRNA was co‐transfected with miR‐494 inhibitor, and then cell viability and migration were redetected. Result showed that, the mRNA level of Gli3 in tumor tissues was higher than in the adjacent tissues (P < 0.01). There were 45 in 99 patients with pancreatic cancer expressed Gli3, and significant correlations were observed between the Gli3 level and vascular invasion (P = 0.04), distant metastasis (P = 0.001), and histologic grade (P = 0.03). Gli3 was a direct target of miR‐494 (P < 0.01) and it was negatively related by miR‐494 (P < 0.01). Overexpression of miR‐494 suppressed PANC‐1 cells viability (P < 0.05, P < 0.01, or P < 0.001) and migration (P < 0.01). Additionally, Gli3 silence suppresses miR‐494 suppression‐induced cell viability and migration (P < 0.01). In conclusion, these data demonstrate miR‐494 exhibits tumor‐suppressive effects on pancreatic cancer, possibly via targeting Gli3.
Examination of interoception along the suicidality continuum
Authors: Rogers Megan L. , Hagan Christopher R. , Joiner Thomas E. 
Published: January 2018

AbstractObjectiveInteroception deficits have been associated with various points on the suicidality continuum. Most research, however, has focused on general, rather than specific facets of, interoception. This study compared individuals with lifetime suicidal ideation, plans, and attempts using a multidimensional assessment of interoception.MethodA total of 537 adults completed measures of their interoception and lifetime suicide histories.ResultsIndividuals with lifetime suicidal ideation reported more worry about their bodily sensations. Those with lifetime suicide attempts reported a greater tendency to distract themselves from bodily sensations and less self‐regulation using bodily sensations. Individuals at all points of the suicidality continuum reported less trust of their bodily sensations.ConclusionThese findings suggest that interoception may be impaired in those with a history of suicidal thoughts and behaviors. Longitudinal designs are needed to parse apart the interplay of these variables and directionality of these relationships.
The acoustics of therapeutic subjectivity and their impact on the resonance of mutual recognition
Authors: Steinfeld Matthew 
Published: January 2018

AbstractThis paper addresses how musical metaphors can be useful in understanding the substructure of psychotherapy by considering how our patients resonate inside us, what that resonance does to and for us, and how these acoustic properties seem to be foundational in the construction of the therapist as an instrument of healing. From this perspective, psychotherapy involves “living music” with another person, as the process of psychotherapy always involves the passing of sound back and forth across an interpersonal divide. This requires both psychotherapist and patient to step into the roles of performer and audience member. Additionally, psychotherapists can be meaningfully thought of not only as an “instrument” of healing, but also an “instrumentalist” who produces unique “therapeutic music.” Moreover, the patient and therapist cocreate the concert space in which treatment is performed. This concert space has unique “architectural,” and thus acoustic, properties within which we hear and remember one another.
Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis
Authors: Yan Keding , Xue Liang , Wang Shouyu 
Published: January 2018

AbstractMicroimaging is of great significance in the biological and medical fields, since it can realize observations acting as important references for cellular research and disease diagnosis. However, traditional microscopy only offers qualitative sample contours; moreover, it is difficult to reach large‐amount sample observations limited by the fixed field of view (FoV). To realize massive cellular measurements quantitatively, three designed quantitative interferometric microscopic cytometers based on the FoV scanning are introduced and compared in details in this article. These devices not only retrieve the quantitative sample phase distributions in the extended FoV, but also provide the detailed information of massive cells, such as cellular volume, area, and roundness. Considering their capabilities as quantitative imaging and large‐amount sampling, it is believed that these quantitative interferometric microscopic cytometers (QIMCs) can be potentially adopted in high‐throughput cell imaging and statistical analysis for both the biological and medical applications.
Differences between serious and nonserious patient safety incidents in the largest hospital district in Finland
Authors: Jämsä Juho Olavi , Palojoki Sari Hannele , Lehtonen Lasse , Tapper Anna‐Maija 
Published: January 2018

ObjectivesTo determine if and in what ways serious patient safety incidents differ from nonserious patient safety incidents.MethodsStatistical analysis was performed on patient safety incident reports that were reported in 2015 in Finland's largest hospital district (Helsinki and Uusimaa, HUS). Reports were divided into two groups: nonserious incidents and serious incidents. Differences between groups were studied from several types of categorically divided information.ResultsOf the total number of reports (15,863), 1% were serious incidents (175). Serious and nonserious incidents differed significantly from each other. Serious incidents concerning laboratory, imaging, or medical equipment were more common. On the other hand, incidents concerning medication, infusion, and blood transfusion were less frequent. In serious incidents, the proportion of doctors reporting was greater, and contributing factors were better recognized, the most common being working of procedures.ConclusionsIn the future, special attention should be given to the particular aspects of serious patient safety incidents, such as safe use of medical equipment, training, and handling of procedures. Root cause analysis is an effective way to handle serious incidents and enables the prevention of their reoccurrence. However, a systematic follow‐up of the root cause analysis should be developed.
Design of nonapeptide LVFFARKHH: A bifunctional agent against Cu2+‐mediated amyloid β‐protein aggregation and cytotoxicity
Authors: Zhang Huan , Zhang Chong , Dong Xiao‐Yan , Zheng Jie , Sun Yan 
Published: January 2018

AbstractDysfunctional accumulation of amyloid β‐protein (Aβ) mediated by Cu2+ exhibits higher neurotoxicity and accelerates the progress of Alzheimer's disease, so inhibition of Cu2+‐mediated Aβ aggregation and cytotoxicity has been considered as a therapeutic strategy for the disease. Herein, a nonapeptide was designed by linking HH to the C‐terminus of a peptide inhibitor of Aβ aggregation, LVFFARK (LK7). We found that the nonapeptide, LK7‐HH, possessed dual functionality, including enhanced inhibition capability on Aβ aggregation as compared to LK7, and chelating Cu2+ with a dissociation constant of 5.50 μM. This enabled LK7‐HH to arrest the generation of reactive oxygen species catalyzed by Cu2+ or Cu2+‐Aβ complex, and to inhibit Cu2+‐induced Aβ aggregation. Moreover, in contrast with the cytotoxicity of LK7 aggregates, LK7‐HH was biocompatible because HH conjugation made its aggregation behavior different from LK7. Thus, LK7‐HH efficiently suppressed Cu2+‐mediated Aβ aggregation and cytotoxicity. An equimolar concentration of LK7‐HH increased cell viability from 50% to 90% when treating Aβ40‐Cu2+ complexes. The results provided insights into the roles of HH in enhancing the inhibition of Aβ and Cu2+‐induced Aβ aggregations, in eliminating Cu2+‐induced cytotoxicities by arresting generation of reactive oxygen species, and in making the peptide biocompatible. Therefore, this work would contribute to the design of potent peptide‐based inhibitors of Cu2+‐mediated Aβ aggregation and cytotoxicity.
Optimal design of analog active filters using symbiotic organisms search
Authors: Dib Nihad , El‐Asir Bassam 
Published: January 2018

AbstractThis paper investigates the optimal design of analog active filters using the symbiotic organisms search (SOS) algorithm. Symbiotic organisms search is a newly proposed global optimization method that is inspired by the symbiotic interaction strategies between different organisms in an ecosystem. In SOS, the 3 common types of symbiotic relationships (mutualism, commensalism, and parasitism) are modeled using simple expressions, which are used to find the global minimum of the fitness function. Unlike other methods, SOS is free of tuning parameters, which makes it an attractive optimization method. Here, SOS is used to determine the values of the passive components (resistors and capacitors) used in active filters. Two active filter topologies, fourth‐order Butterworth low‐pass filter and second‐order state variable low‐pass filter, are considered. The components values are chosen from several standard industrial series (E12, E24, and E96). The results obtained using SOS are compared with those obtained using several optimization methods, like particle swarm optimization, seeker optimization algorithm, differential evolution, and other ones. It is shown that the SOS is a robust straightforward evolutionary algorithm that can outperform other well‐known methods.
The influence of the Pacific Decadal Oscillation on North Central China precipitation during boreal autumn
Authors: Qin Minhua , Li Dongliang , Dai Aiguo , Hua Wenjian , Ma Hedi 
Published: January 2018

ABSTRACTDecadal variations in autumn (September–October–November, SON) precipitation over North Central China since the 1940s are investigated using observations and reanalysis data. They are found to be significantly correlated negatively with the Pacific Decadal Oscillation (PDO). Further analyses indicate that the inter‐decadal changes in SON precipitation over North Central China result from anomaly patterns in the large‐scale atmospheric circulation in the mid‐latitude Northern Hemisphere associated with the PDO. Specifically, during the negative phase of the PDO (1945–1976 and 2003–2014), an anomalous low pressure and cyclonic circulation are seen west of Lake Baikal, while positive pressure anomalies are located over the East Asia‐Japan‐North Pacific region, which weakens the East Asian trough (EAT). The weakened EAT favours southerly winds over North Central China. The negative pressure anomalies around Mongolia and Lake Baikal induce large‐scale ascent motion to the west of the EAT, which also produces southerly advection of warm and moist air into North Central China, leading to increased precipitation there. These results reinforce the notion that PDO has a large impact on SON rainfall over North Central China on decadal timescales and highlight the need to further examine how the PDO induces the Eurasia atmospheric teleconnection pattern (e.g. East Atlantic/Western Russia pattern).
Insulin Instability in Parenteral Nutrition Admixtures
Authors: Forchielli Maria Luisa , Bongiovanni Franco , Platé Livia , Piazza Giulia , Puggioli Cristina , D'Alise Antonietta , Bersani Germana 
Published: January 2018

AbstractBackgroundBiosynthetic human recombinant short‐acting insulin is added to parenteral nutrition (PN) admixtures to nourish glucose‐intolerant patients. Insulin, however, is electrostatically attracted and inactivated by ethyl‐vinyl‐acetate (EVA) bags and filling system tubes. Our aim was to verify and quantify the presence of insulin in PN with and without intravenous lipid emulsion (ILE), just after addition (T0) until the infusion's end (T24).MethodsFour undiluted samples of 12 different PN complete admixtures (6 with ILE and 6 without), each containing 250 g of glucose in a 2000 mL volume, were taken and analyzed at T0 and T24 by an automated electrochemiluminescence immunoassay after the addition of biosynthetic human recombinant short‐acting insulin at increasing doses (from 6 to 72 IU/bag) by an automated compounding device. Assay sensitivity was set at 2 μIU/mL. Admixtures with and without ILE were compared in terms of insulin‐detected amounts at T0 and T24.ResultsRegardless of the amount initially provided, insulin was missing in PN without ILE. In admixtures with ILE, the greater the insulin and ILE doses initially included, the higher the insulin availability at T0 and T24, both in absolute terms and as a percentage of the initial amount (from 3 to 81% at T0 and from 2.5 to 72.5% at T24). ILE may prevent insulin attraction to plastic surfaces.ConclusionsInsulin is recovered in the presence of ILE in PN even though considerable amounts are untraceable. This aspect needs verification. Until then, insulin should safely be injected in a different manner in uncontrolled situations.
Effect of Delaying Replacement of Parenteral Nutrition Intravenous Administration Sets: Preclinical Experiments and a Dynamic Laboratory Model of Microbial Colonization
Authors: Gavin Nicole Clare , McMillan David , Keogh Samantha , Choudhury Md Abu , Ray‐Barruel Gillian , Rickard Claire M. 
Published: January 2018

AbstractBackgroundRecommendations prescribe daily intravenous administration set (IVAS) replacement for parenteral nutrition (PN) comprising intravenous fat emulsions (IVFE) due to risk of micro‐organism growth and resultant central‐line associated bloodstream infections (CLABSIs), but system disconnection for this practice may allow contamination and CLABSIs.Materials and MethodsLaboratory experiments and model development were used to simulate PN administration after contamination from healthcare workers’ hands. This study observed the growth of micro‐organisms known to cause CLABSIs in a variety of PN and other IV fluids and developed a model to investigate the effect of delaying IVAS replacement on microbial growth for up to 7 days.ResultsMicro‐organisms grew at different rates and were affected by solution type. In static experiments, growth was supported in IVFE and all‐in‐one PN, but suppressed in 50% glucose. Growth patterns were consistent over time for Staphylococcus epidermidis, Staphylococcus aureus, and Candida albicans in IVFE, all‐in‐one PN, and 0.9% sodium chloride in both static and dynamic experiments. C. albicans grew exponentially to clinically significant numbers in all‐in‐one PN and IVFE IVAS after 30 hours, but negligible growth of S. epidermidis or S. aureus occurred for 7 days.ConclusionAll‐in‐one PN and IVFE support the C. albicans growth after minimal initial contamination, with micro‐organisms migrating from the fluid bag to the central venous access device. Improved aseptic nontouch technique during clinical practice is vital to prevent contamination. Daily IVAS replacement of for all‐in‐one PN and IVFE should continue until the safety of prolonging IVAS replacement is confirmed by randomized trials.
A New Model for Non‐Lipid Compounded Neonatal Parenteral Nutrition Solution Osmolality
Authors: Borenstein Sivan , Mack Ellen , Palmer Katherine , Cat Tram , Gibson Leena Caroline , Sandhu Meenu , Wang Jinyuan , Simmons Charles F. 
Published: January 2018

AbstractBackgroundOsmotic stress is a physical risk factor for adverse events related to peripheral parenteral nutrition (PN) administration, such as infiltration. We sought to improve prediction of compounded PN osmolality utilizing basic nutrient solutions available to North American neonatal intensive care units. This study tested the hypothesis that calculated osmolarity underestimates osmolality in compounded PN.MethodsOsmolarity (mOsm/L) was calculated utilizing commercial software. Osmolality (mOsm/kg) was determined by a freezing‐point depression micro‐osmometer. The relationship between calculated osmolarity and measured osmolality was modeled from linear or polynomial regression analysis using the least squares method. Regression models were based upon calculated osmolarity and included various combinations of PN components.ResultsCalculated osmolarity significantly underestimated measured osmolality in all PN samples (n = 363). Based upon the osmolality of PN and the basic nutrient solutions, we determined a polynomial regression that effectively corrects for the osmolal gap (measured osmolality‐calculated osmolarity) in the validation set (R2 = 0.99367). The unbiased analysis corrected for the osmolal gap based on individual solute behaviors, as well as the solute‐solute interactions in compounded solutions.ConclusionsCalculated osmolarity (mOsm/L) significantly underestimates the osmolality (mOsm/kg) in compounded PN. We developed a new algorithm to more accurately predict PN osmolality based upon calculated osmolarity from commercial software and composition of neonatal basic nutrient solutions used in North America. We propose that use of this PN algorithm will facilitate future studies to determine whether a causal association exists between PN osmolality and adverse events, and to establish safe thresholds for PN concentration in neonates.
Statistical modeling for estimating glucosinolate content in Chinese cabbage by growth conditions
Authors: Kim Do‐Gyun , Shim Joon‐Yong , Ko Myung‐Jun , Chung Sun‐Ok , Chowdhury Milon , Lee Wang‐Hee 
Published: January 2018

AbstractBACKGROUNDGlucosinolate in Chinese cabbage (Brassica campestris L. ssp. pekinensis (Lour.) Rupr) has potential benefits for human health, and its content is affected by growth conditions. In this study, we used a statistical model to identify the relationship between glucosinolate content and growth conditions, and to predict glucosinolate content in Chinese cabbage.RESULTMultiple regression analysis was employed to develop the model's growth condition parameters of growing period, temperature, humidity and glucosinolate content measured in Chinese cabbage grown in a plant factory. The developed model was represented by a second‐order multi‐polynomial equation with two independent parameters: growth duration and temperature (adjusted R2 = 0.81), and accurately predicted glucosinolate content after 14 days of seeding.CONCLUSIONTo our knowledge, this study presents the first statistical model for evaluating glucosinolate content, suggesting a useful methodology for designing glucosinolate‐related experiments, and optimizing glucosinolate content in Chinese cabbage cultivation. © 2018 Society of Chemical Industry
Soil organic matter doubles the cation exchange capacity of tropical soil under no‐till farming in Brazil
Authors: Ramos Fabricio T , Dores Eliana FG de Carvalho , Weber Oscarlina L dos Santos , Beber Daniel C , Campelo José H , Maia João C de Souza 
Published: January 2018

AbstractBACKGROUNDAgricultural conservation practices increase total organic carbon storage in soil (TOCS), a factor that is correlated with the physical and chemical qualities of highly weathered soils. In this study, we investigated the effects of TOCS on the physicochemical attributes of a Latosol after 10 years of no‐till management in Mato Grosso State, Brazil.RESULTSTCOS was highly correlated (r = 0.92) with cation exchange capacity (CEC, pH = 7) and soil density. In the top 0.2 m soil layer, CEC increased by 25% with every 1.8 kg m−2 of stored organic carbon. Eliminating soil organic matter reduced CEC from an already low value of 8.40 cmolc kg−1 to 4.82 cmolc kg−1. Humus is therefore clearly important for the formation of a negative liquid charge in a predominantly electropositive but clayey soil.CONCLUSIONWe confirmed that TOCS is an indicator related to the physiochemical characteristics of weathered soils. Furthermore, our results demonstrate that the increased carbon storage under non‐tilling systems is essential for guaranteeing weathered soil fertility in tropical climates. © 2018 Society of Chemical Industry
Relationships between tyrosine, phenylalanine, chlorogenic acid, and ascorbic acid concentrations and blackspot biochemical potential and blackspot susceptibility in stored russet potatoes
Authors: Goyer Aymeric , Pellé Julien 
Published: January 2018

AbstractBACKGROUNDBlackspot in potato is an internal tissue discoloration that occurs during handling and transport of potato tubers. Blackspot is cosmetically undesirable and represents a huge economic cost for the potato industry. The aim of this study was to test whether concentrations of certain metabolites in the potato tuber cortex could predict blackspot susceptibility.RESULTSSeven russet potato varieties were stored for eight months at 8.8 °C. Stored tubers were subjected to mechanical impact and evaluated for blackspot susceptibility. A blackspot susceptibility index was calculated for each variety by determining an index for the percentage of the tuber cortex area that was covered with blackspot, and an index for the intensity of blackspot discoloration. Concentrations of tyrosine, chlorogenic acid, phenylalanine, and ascorbic acid, and blackspot biochemical potential of tubers to synthesize pigments were measured in the tuber cortex. Blackspot indices, metabolites concentrations and blackspot biochemical potential varied significantly between varieties. Tyrosine concentrations strongly, significantly, and positively correlated with blackspot biochemical potential. Phenylalanine concentrations showed good, significant, and positive correlation with blackspot biochemical potential and discoloration index. None of the analyzed metabolites correlated with blackspot susceptibility.CONCLUSIONConcentrations of tyrosine and phenylalanine explained up to ∼80% of the variation in blackspot biochemical potential between varieties but did not correlate with blackspot susceptibility. © 2018 Society of Chemical Industry