bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moisture‐resistant nitroaromatic explosive gas sensor based on hydrophilic pentiptycene polymer
Authors: Kim Gyeongsoo , Lee Sun Bu , Heo Jaeyoung , An Tae Eun , Lee Gang Min , Kim Junggong , Kim Keunyoung , Lee Jongman , Bae Han Yong , Song Changsik 
Published: October 2024

AbstractThe detection of explosive materials, particularly nitroaromatic compounds such as 2,4,6‐trinitrotoluene (TNT), is critical for public safety and national security. Existing detection methods often require complex instrumentation, limiting their applicability for real‐time or in‐field use. Fluorescent sensors, which offer rapid and sensitive detection through fluorescence quenching, present a promising alternative. This study evaluates the performance of two pentiptycene‐based polymers, P‐1 and P‐2, in detecting explosive vapors, with a specific focus on their behavior under high‐humidity conditions. P‐2, modified with triethylene glycol groups, demonstrated significantly increased hydrophilicity compared to the commonly studied P‐1, as confirmed by contact angle measurements. Spectroscopic analysis revealed a blue shift in P‐2's UV–Vis absorption and photoluminescence spectra, indicating electronic property changes due to structural modifications. The enhanced hydrophilicity of P‐2 enabled it to maintain stable sensing performance under moist conditions, showing less than a 10% reduction in sensitivity, compared to the 20% decrease observed in P‐1. This superior moisture resistance suggests that P‐2 is a more robust and practical sensor for explosive detection in environments with variable humidity levels.
An undergraduate research experience in CRISPR‐Cas9 mediated eukaryotic genome editing to teach fundamental biochemistry techniques
Authors: Tonsager Andrew J. , Stargell Laurie A. 
Published: October 2024

AbstractCRISPR‐Cas9 technology is an established, powerful tool for genome editing through the ability to target specific DNA sequences of interest for introduction of desired genetic modifications. CRISPR‐Cas9 is utilized for a variety of purposes, ranging from a research molecular biology tool to treatment for human diseases. Due to its prominence across a variety of applications, it is critical that undergraduates in the life sciences are educated on CRISPR‐Cas9 technology. To this end, we created an intensive eight‐week long course‐based undergraduate research experience (CURE) designed for students to understand CRISPR‐Cas9 genome editing and perform it in Saccharomyces cerevisiae. Students enrolled in the CURE participate in 2, 3‐h sessions a week and are engaged in the entire process of CRISPR‐Cas9 genome editing, from preparation of genome editing reagents to characterization of mutant yeast strains. During the process, students master fundamental techniques in the life sciences, including sterile technique, Polymerase Chain Reaction (PCR), primer design, sequencing requirements, and data analysis. The course is developed with flexibility in the schedule for repetition of techniques in the event of a failed experiment, providing an authentic research experience for the students. Additionally, we have developed the course to be easily modified for the editing of any yeast gene, offering the potential to expand the course in research‐driven classroom or laboratory settings.
A BreakoutEDU for understanding gene expression in high school. A case study: Design, implementation, and evaluation of the emotions of its implementation
Authors: Martínez‐Carmona Marina , Ayuso Fernández G. Enrique , Fernández‐Díaz Manuel , Serrano‐García Francisco , Griñán Antonia Plaza 
Published: October 2024

AbstractKnowledge about genetics is essential to build a society capable of participating in socioscientific and ethical debates. However, this subject remains difficult for students, making it necessary to develop new educational strategies, such as gamification. Thus, two main objectives are established in this work: (a) to design and evaluate BreakoutEDU, a gamified activity to improve understanding of the content of gene expression; and (b) to study the emotions triggered by this activity among students. Using questionnaires and observation templates, the implementation of BreakoutEDU is evaluated in two groups of first‐year high school students (15 and 10 students). The results are analyzed qualitatively and quantitatively. Bearing in mind the limitations of this study, it is concluded that the designed BreakoutEDU could support gamification as a good strategy to contextualize and approach the abstract content of gene expression. Moreover, the activity could maintain a balance between time, difficulty and students' skills, encourage teamwork and trigger mainly positive emotions.
E!Canasta: A card game to teach ATP synthesis and metabolic regulation in biochemistry classes
Authors: Faria de Souza Thalles Henrique , Figueiredo Peloso Eduardo , Gerber Hornink Gabriel 
Published: October 2024

AbstractUnderstanding ATP formation is essential for learning metabolism and is central to grasping metabolic processes as a whole. However, due to the high level of abstraction, the number of intermediate substrates, the connections, and integrated regulation, its comprehension often poses a challenge. This and the fact that traditional teaching methods struggle when dealing with highly abstract concepts, game‐based strategies present a more concrete and dynamic alternative, which led to the creation of E!Canasta (card game). Developed based on Canasta and adapted in order to improve the learning of concepts, including some of pathway's regulation and integration, E!Canasta motivates students and promotes engagement in a fun activity. Students assemble a sequence of cards representing the glycolysis, acetyl‐CoA, Krebs cycle, and electron transport chain, which correspond to the card suits. Strategically, some of the cards hold special feats that simulate some aspects of metabolic regulation and integration (to give or take away points). At the end of the game, points are added up for sequences and cards with positive or negative effects. The game was played with two classes of students enrolled in biochemistry as part of their graduations (86 players). Student perception on gameplay, motivation and understanding was measured through an anonymous Likert scale questionnaire, with very positive results in all questions. Statistically significant correlations were observed regarding the perceived comprehension of pathways and their regulation, and in linking motivation with a positive gaming experience, showcasing that E!Canasta demonstrates considerable educational potential, along with an enjoyable experience for learning ATP synthesis.
Quantitation of the DNA‐dependent protein kinase inhibitor peposertib (M3814) and metabolite in human plasma by LC–MS/MS
Authors: Christner Susan M. , Parise Robert A. , Bakkenist Christopher J. , Davis S. Lindsey , Feng Ye , Synold Timothy , Gore Steven , Beumer Jan H. 
Published: October 2024

AbstractThe DNA‐dependent protein kinase (DNA‐PK) is an abundant nuclear protein that mediates DNA double‐strand break repair by nonhomologous end joining (NHEJ). As such, DNA‐PK is critical for V(D)J recombination in lymphocytes and for survival in cells exposed to ionizing radiation and clastogens. Peposertib (M3814) is a small molecule DNA‐PK inhibitor currently in preclinical and clinical development for cancer treatment. We have developed a high‐performance liquid chromatography‐mass spectrometry method for quantitating peposertib and its metabolite in 0.1 mL human plasma. After MTBE liquid–liquid extraction, chromatographic separation was achieved with a Phenomenex Synergi polar reverse phase (4 μm, 2 × 50 mm) column and a gradient of 0.1% formic acid in acetonitrile and water over an 8 min run time. Mass spectrometric detection was performed on an ABI SCIEX 4000 with electrospray, positive‐mode ionization. The assay was linear from 10 to 3000 ng/mL for peposertib and 1–300 ng/mL for the metabolite and proved to be both accurate (97.3%–103.7%) and precise (<8.9%CV) fulfilling criteria from the Food and Drug Administration (FDA) guidance on bioanalytical method validation. This liquid chromatography‐tandem mass spectroscopy (LC–MS/MS) assay will support several ongoing clinical studies by defining peposertib pharmacokinetics.
Why Do Some Depressive Patients Have Suicidal Ideation but Others Not? Suicidal Ideation From the Perspective of Affective Neuroscience Personality Traits
Authors: Hoşgören Alici Yasemin , Ceran Selvi , Hasanli Jamal , Asut Gonca , Özel Beren , Ucar Hasanli Zehra , Saygi Gökçe , Bağcaz Arda , Misir Emre 
Published: October 2024

ABSTRACTIntroductionAlthough major depression is the disorder most frequently associated with suicidal behavior, it is unclear that major depressive disorder patients may develop suicidal ideation. Basic affective system theory may provide a novel and beneficial viewpoint in this field. The goal of this study was to investigate the basic affective system in relation to suicidal ideation in individuals with depression.MethodThe study population comprised 160 participants who had been formally diagnosed with major depressive disorder. Participants were divided into two groups according to whether they had suicide ideation (depression with suicide [DS]) (N = 93) or not (depression with no suicidal ideation [DNS]) (N = 67). The Beck Depression Inventory (BDI), the Suicide Probability Scale (SPS), the Beck Hopelessness Scale (BHS), and the Affective Neuroscience Personality Scale (ANPS) were applied. Statistical Product and Service Solutions (SPSS) 24 and the SPSS macroprocess, which were specifically developed for assessing complex models including serial mediators, were used to analyze the data.ResultsThe mean age of all participants was 31.1 ± 11 years, and most of them were female (65%). The DS group had a higher family history of psychiatric medication use and suicidal attempts. In addition, BDI, BHS, and SPS total scores were higher, as well as ANGER subscale scores were higher in the DS group. In mediation analysis, the ANGER subscale significantly predicted the presence of suicide ideation. We observed a direct effect of the ANGER subscale score on suicide ideation as well as an indirect effect of the ANGER subscale score on suicidal ideation via depression severity.ConclusionHigher scores on ANGER are associated with suicidal ideation. Neurobiological correlates, including the ANGER system, may be promising in understanding suicidal behaviors.
The Relationship Between Attention and Suicidal Ideation Among Patients With Adult‐Onset Chronic Schizophrenia
Authors: Wang Qiongzhang , Zhou Yurou , Xu Weiqian , Tang Wei , Liu Jing 
Published: October 2024

ABSTRACTBackgroundPatients with chronic schizophrenia (SZ) have a high risk of suicide, and their cognition function is impaired. We aimed to explore the relationship between attention and suicidal ideation among patients with adult‐onset chronic SZ.MethodsA total of 416 patients with adult‐onset chronic SZ were enrolled in this study. We divided patients into suicidal ideation group and non‐suicidal ideation group according to the evaluation results of the Beck Scale for Suicide Ideation. Psychotic symptoms were measured by Positive and Negative Syndrome Scale (PANSS), and cognitive function was measured by Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). Insomnia symptoms were measured by Insomnia Severity Index (ISI).ResultsAge was significantly different (44.28 ± 10.58 vs. 48.46 ± 12.23, t = 10.64, p = 0.001) between the two groups, and the patients with suicidal ideation were younger than patients without suicidal ideation. The positive symptom scores of the PANSS, the scores of ISI, and attention scores of RBANS were higher in patients with suicidal ideation than patients without suicidal ideation (17.30 ± 5.67 vs. 15.58 ± 4.90, t = 9.633, p = 0.002; 3.00 [1.00–6.00] vs. 2.00 [1.00–3.50], Z = −2.048, p = 0.041; 81.80 ± 14.99 vs. 76.91 ± 13.88, t = 10.101, p = 0.002). In the logistic regression analysis, age (odds ratio [OR], 0.973; 95% confidence interval [95%CI], [0.955–0.992]; p = 0.005), positive symptom scores of PANSS (OR, 1.063; 95%CI, [1.019–1.109]; p = 0.005), ISI scores (OR, 1.098; 95%CI, [1.037–1.163]; p = 0.001), and attention scores of RBANS (OR, 1.029; 95%CI, [1.013–1.047]; p = 0.001) were independently associated with the occurrence of suicidal ideation among patients with adult‐onset chronic SZ.ConclusionsHigh attention scores of RBANS were a risk factor for suicidal ideation among patients with adult‐onset chronic SZ.
Industry 4.0‐driven business model innovation for supply chain sustainability: An exploratory case study
Authors: Krishnan Ramesh , Phan Phi Yen , Krishnan S. Navaneetha , Agarwal Renu , Sohal Amrik 
Published: October 2024

AbstractIn the rapidly evolving landscape of the automotive industry, firms are increasingly turning to advanced Industry 4.0 (I4.0) technologies to drive innovation and sustainability. While I4.0 technologies hold immense potential for Business Model Innovation (BMI) and supply chain (SC) sustainability, a gap exists in understanding how firms can leverage BMI and SCS effectively. This study addresses this gap and explores the impact of implementing I4.0 on BMI and its effects on SC sustainability. Utilizing an exploratory case study approach, the research investigates an Indian automobile SC and highlights how I4.0 technologies such as IoT, cloud computing, additive manufacturing, analytics, and automation contribute to BMI, enhancing operational efficiency and SC sustainability. Additionally, the study emphasizes the importance of fostering collaboration among SC entities and the need for support from large‐scale manufacturers for the initial adoption of I4.0 technologies at the small‐scale supplier level. Based on these findings, the study develops the ‘I4.0‐enabled BMI for SC Sustainability’ framework, providing a structured approach for integrating I4.0 and BMI to enhance SC sustainability. This research investigates and applies Dynamic Capability (DC) theory to examine I4.0 transformations and contributes to DC theory by demonstrating how I4.0 facilitates BMI across the value chain, leading to improved sustainability performance.
Visualization of the dentogingival junction using micro‐plastination technique
Authors: Correa‐Aravena Jaime , Panes Camila , Ponce Nikol , Prado‐Sanhueza Aurora , Guzmán Diego , Vásquez Bélgica , Roa Ignacio , Veuthey Carlos , Masuko Telma S. , Ottone Nicolás E. 
Published: October 2024

AbstractPlastination has revolutionized the field of anatomy and research by providing biosecurity and enabling the long‐term preservation of biological material, ranging from entire bodies to individual organs and even micron sections. The dentogingival junction (DGJ) consists of both epithelial and connective tissues that are closely related to the tooth's mineralized tissues. Cutting‐grinding techniques are commonly used to visualize DGJ histology. These techniques exclude enamel from preparations and focus on visualizing hard or soft tissues. To improve the micro‐anatomical and histological study of this region, we applied micro‐plastination technique to obtain micro‐thin slices below 150 μm thick from human and animal samples. The DGJ microanatomy was visualized by applying histological stains to the micro‐plastinated slices, highlighting the technique's endogenous autofluorescence capacity identifying periodontal tissues, including dentin, enamel, cementoenamel junction, dentinal tubules, connective tissue, and collagen. Based on our results, we confirm that micro‐plastination is a useful technique for visualizing anatomical regions that are difficult to access, such as the DGJ. Micro‐plastination can be used as an alternative technique, providing a new approach for its application in anatomical and morphological research protocols.
CD11b/CD86 involved in the microenvironment of colorectal cancer by promoting Wnt signaling activation
Authors: Ke Junyu , Chen Guirong , You Yihui , Xie Qinghua , Liu Zheng‐lin , Song Chunhui , Zheng Yanqiu , Shan Zejun , Song Jinbin , Jiang Zhangyu , Wang Haiyan , Du Qun , Wu Yongqiang , Chen Xin‐lin , Li Yanwu 
Published: October 2024

AbstractBackgroundColorectal cancer (CRC) is a malignancy that arises within the gastrointestinal tract. Despite ongoing research, the etiology and pathogenesis of CRC remain elusive; particularly, the distribution and characteristics of tumor‐associated macrophages is currently an active area of investigation in understanding the pathological progression and prevention of CRC.MethodsThis study utilized CRC patient surgical samples, mouse models of colitis‐associated cancer, colonic organoid, and co‐culture cell line to examine the changes in CD11b/CD86 at different pathological region and detect the Wnt signaling pathway activity.ResultsOur findings revealed a sensitive and increased expression of CD11b from the early to the advanced CRC tissues and correlated with poor prognosis, while CD86 expression was reduced in advanced CRC tissues. CD133 expression was also elevated in advanced CRC tissues and mainly co‐localized with CD11b, suggesting a positive regulatory effect of CD11b and CD133 expression that may contribute to CRC progression. In AOM/DSS mouse models, activation of the Wnt signaling pathway was associated with increased CD133 and CD11b expression. In vitro, THP‐1 cell was induced to high expression of CD11b, and the above conditional cultural medium enhanced HCT116 cell colony number and CD133 protein expression. Furthermore, colonic crypts from AOM/DSS mouse models were isolated to culture, and the colonic organoids exhibited dilation and significant increases expression of CD133 and β‐Catenin/N‐P‐B‐Catenin.ConclusionsCD11b might be an important factor to participate the progress of CRC. And the high CD11b of CRC microenviroment might potentially promote CD133 expression and associate with Wnt signal activation.
Antibacterial Activity of Glycosylated Flavonoids Isolated from Ocotea notata Leaves
Authors: de Jesus Borges Costa Isabela Francisca , Rocha Reis João Vitor , Ungaretti Paleo Konno Tatiana , Wanderley Tinoco Luzineide , Correa Pinto Shaft , Correa Ramos Leal Ivana , Frazão Muzitano Michelle 
Published: October 2024

AbstractResearch into natural products has led to the discovery of new drugs. This work shows relevant data on the antibacterial activity of Ocotea notata leaves. Dichloromethane fraction (DF) from leaves of O. notata showed antibacterial activity at the concentration of 512 μg/mL against strains of Staphylococcus. The DF was fractionated to investigate bioactive compounds and yielded six subfractions (DF1‐DF6). The DF3, DF5, and DF6 showed bacteriostatic activity, and DF4 showed bactericidal activity at the concentration of 2048 μg/mL and additive effect when combined with the antibiotic oxacillin. The flavonoids miquelianin (1), isoquercitrin (2), reynoutrin (3), guaijaverin (4), and afzelin (5) were isolated from the DF4 and characterized via NMR and HPLC‐DAD‐MS/MS analyses. Flavonoids 1‐5, especially reynoutrin (3), seem to be responsible for the DF4 antibacterial activity. Reynoutrin showed activity against Staphylococcus epidermidis ATCC 12228 and S. aureus methicillin‐resistant ATCC 33591. This is the first report regarding the reynoutrin antibacterial activity and the first description of the flavonoid guaijaverin for O. notata.
Three New Antibacterial Mycophenolic Acid Derivatives from the Marine‐Derived Fungus Penicillium sp. HN‐66
Authors: Mo TuXiang , Qin YuYue , Zhang Yue , Liang YongBin , Li XiaoBao , Li WanShan , Chen GuangYing 
Published: October 2024

AbstractThree new mycophenolic acid derivatives, penicacids L−N (1–3), together with four known analogues, were isolated from a fungus Penicillium sp. HN‐66 derived from a South China Sea marine sediment. The structures of compounds 1–3 were determined on the basis of HR‐ESI‐MS, NMR (1H, 13C, HSQC and HMBC) data analyses, and comparison of optical rotations. Antimicrobial activities of 1–7 were tested. The results showed that compounds 1–3 and 5–7 had weak inhibitory effects against E. coli ATCC 25922 with the MIC values of 50 μg/mL.
Exploring Phytochemical Profile and Biological Activities of Callistemon viminalis (Sol. ex Gaertn.) G.Don ex Loudon) By In vitro and In silico Approaches
Authors: Kanwal Shamsa , Ahmad Saeed , Rao Huma , Khan Kashif ur Rehman , Zeeshan Muhammad , Farooq Samra 
Published: October 2024

AbstractCallistemon viminalis (Sol. ex Gaertn.) G.Don ex Loudon) (family: Myrtaceae) is used for its medicinal properties in treating various metabolic disorders. We investigated the chemical characterization and biological screening of the n‐hexane extract of C. viminalis. The total phenolic content was (37.45±7.40 mg GA.E/g D.E±S.D) and the total flavonoid content was (18.43±6.34 mg R.E/g D.E±S.D). GC‐MS screening of the n‐hexane extract tentatively identified 70 bioactive phytochemicals. The maximum antioxidant potential (289.99±9.01 mg T.E/g D.E±S.D) was observed via the FRAP assay. Enzyme inhibition assays revealed that n‐hexane extract of C. viminalis showed enzyme inhibition against the enzymes including α‐glucosidase (6.9±0.13 mmol of ACA.E/g D.E±S.D); α‐amylase (7.2±0.56 mmol of ACA.E/g D.E±S.D), urease (4.95±0.9 mg of TU.E/g D.E±S.D), acetylcholinesterase (2.9±0.08 mg GALA.E/g D.E±S.D), lipoxygenase (4.93±1.05 mg of Indo.E/g D.E±S.D) and tyrosinase (4.33±0.62 mg of KA.E/g D.E±S.D). The extract showed maximum antibacterial activity against Staphylococcus aureus (71.30±4.44 %) followed by Bacillus subtilis (68.55±2.70 %), Pseudomonas aeruginosa (57.86±6.02 %), and Salmonella typhi (53.90±5.05 %). Docking studies revealed good docking interactions between ligands and the studied enzymes, whereas ADME analysis revealed the pharmacokinetic profiles of the phytoconstituents. C. viminalis possesses promising therapeutic potential and can be further explored for drug development and drug design.
Comparative Analysis of Thermal Activation on Felts and Continuous Carbon Filament Electrodes for Vanadium Redox Flow Batteries
Authors: Aguiló‐Aguayo Noemí , Ebert Toni Alena , Amade Roger , Bertran Enric , Ospina Rogelio , Rodriguez‐Pereira Jhonatan , Ponce de León Carlos , Bechtold Thomas , Pham Tung 
Published: October 2024

AbstractThermal treatments are commonly used to improve electrode kinetics in vanadium redox flow batteries (VRFB). The impact of the widely adopted thermal treatment—400 °C for least 24 hours—was investigated on polyacrylonitrile (PAN)‐based continuous carbon filaments (tows) and compared to PAN‐based graphite felts. Surface properties were assessed with scanning electron microscopy (SEM), Raman spectroscopy, X‐ray photoelectron spectroscopy (XPS) and wettability measurements. The electrode activity was investigated via electrochemical impedance spectroscopy (EIS). Charge‐transfer resistances and the constant phase element parameters related to the electric double layer were determined, revealing a correlation between enhanced electrode activity and increased double layer across all electrodes. An 8‐hour 400 °C thermal treatment was sufficient to improve electrode activity for tows, whereas felts required longer durations, up to 24 hours, attributed to differences in the carbonization process employed for each material, with the tows undergoing continuous processing and the felts following a batch process. Three‐electrode half‐cell EIS measurements were conducted to elucidate positive and negative electrode contributions. Activated continuous carbon filament electrodes exhibited consistent electrode activities in both the catholyte (VO2+/VO2+) and anolyte (V3+/V2+), whereas the electrochemical activity of felts was limited by the electrode deactivation in the anolyte.