bluuebunny/crossref_metadata_2025_split huggingface dataset:
High ambient temperature facilitates the acquisition of 3,4-methylenedioxymethamphetamine (MDMA) self-administration
Authors: Aarde Shawn. M. , Huang Pai-Kai , Taffe Michael A. 
Published: April 2017

AbstractRationaleMDMA alters body temperature in rats with a direction that depends on the ambient temperature (TA). The thermoregulatory effects of MDMA and TAmay affect intravenous self-administration (IVSA) of MDMA but limited prior reports conflict.ObjectiveTo determine how body temperature responses under high and low TAinfluence MDMA IVSA.MethodsMale Sprague-Dawley rats were trained to IVSA MDMA (1.0 mg/kg/infusion; 2-hr sessions; FR5 schedule of reinforcement) under TA20°C or 30°C. Radiotelemetry transmitters recorded body temperature and activity during IVSA.ResultsMDMA intake increased under both TAduring acquisition, but to a greater extent in the 30°C group. The magnitude of hypothermia was initially equivalent between groups but diminished over training in the 30°C group. Within-session activity was initially lower in the 30° C group, but by the end of acquisition and maintenance, activity was similar for both groups. When TAconditions were swapped, the hot-trained group increased MDMA IVSA under 20 °C TAand a modest decrease in drug intake was observed in the cold-trained group under 30 °C TA. Subsequent non-contingent MDMA (1.0-5.0 mg/kg, i.v.) found that rats with higher MDMA IVSA rates showed blunted hypothermia compared with rats with lower IVSA levels; however, within-session activity did not differ by group. High TAincreased intracranial self-stimulation thresholds in a different group of rats and MDMA reduced thresholds below baseline at low, but not high, TA.ConclusionsHigh TAappears to enhance acquisition of MDMA IVSA through an aversive effect and not via thermoregulatory motivation.
Phylogenetic Conflict in Bears Identified by Automated Discovery of Transposable Element Insertions in Low Coverage Genomes
Authors: Lammers Fritjof , Gallus Susanne , Janke Axel , Nilsson Maria A 
Published: April 2017

AbstractCompared to sequence analyses, phylogenetic reconstruction from transposable elements (TEs) offers an additional perspective to study evolutionary processes. However, detecting phylogenetically informative TE insertions requires tedious experimental work, limiting the power of phylogenetic inference. Here, we analyzed the genomes of seven bear species using high throughput sequencing data to detect thousands of TE insertions. The newly developed pipeline for TE detection called TeddyPi (TE detection and discovery for Phylogenetic Inference) obtained 150,513 high-quality TE insertions in the genomes of ursine and tremarctine bears. By integrating different TE insertion callers and using a stringent filtering approach, the TeddyPi pipeline produced highly reliable TE insertion calls, which were confirmed by extensivein vitrovalidation experiments. Screening for single nucleotide substitutions in the flanking regions of the TEs show that these substitutions correlate with the phylogenetic signal from the TE insertions. Our phylogenomic analyses show that TEs are a major driver of genomic variation in bears and enabled phylogenetic reconstruction of a well-resolved species tree, even with strong signals for incomplete lineage sorting and introgression. The analyses show that the Asiatic black, sun and sloth bear form a monophyletic clade. TeddyPi is open source and can be adapted to various TE and structural variation callers. The pipeline makes it easy to confidently extract thousands of TE insertions even from low coverage genomes of non-model organisms, opening new possibilities for biologists to study phylogenies, evolutionary processes as well as rates and patterns of (retro-)transposition and structural variation.
PREDICTD: PaRallel Epigenomics Data Imputation with Cloud-based Tensor Decomposition
Authors: Durham Timothy J. , Libbrecht Maxwell W. , Howbert J. Jeffry , Bilmes Jeff , Stafford Noble William 
Published: April 2017

AbstractThe Encyclopedia of DNA Elements (ENCODE) and the Roadmap Epigenomics Project have produced thousands of data sets mapping the epigenome in hundreds of cell types. However, the number of cell types remains too great to comprehensively map given current time and financial constraints. We present a method, PaRallel Epigenomics Data Imputation with Cloud-based Tensor Decomposition (PREDICTD), to address this issue by computationally imputing missing experiments in collections of epigenomics experiments. PREDICTD leverages an intuitive and natural model called “tensor decomposition” to impute many experiments simultaneously. Compared with the current state-of-the-art method, ChromImpute, PREDICTD produces lower overall mean squared error, and combining methods yields further improvement. We show that PREDICTD data can be used to investigate enhancer biology at non-coding human accelerated regions. PREDICTD provides reference imputed data sets and open-source software for investigating new cell types, and demonstrates the utility of tensor decomposition and cloud computing, two technologies increasingly applicable in bioinformatics.
Uncovering the repertoire of endogenous flaviviral elements inAedesmosquito genomes
Authors: Suzuki Yasutsugu , Frangeul Lionel , Dickson Laura B. , Blanc Hervé , Verdier Yann , Vinh Joelle , Lambrechts Louis , Saleh Maria-Carla 
Published: April 2017

AbstractEndogenous viral elements derived from non-retroviral RNA viruses were described in various animal genomes. Whether they have a biological function such as host immune protection against related viruses is a field of intense study. Here, we investigated the repertoire of endogenous flaviviral elements (EFVEs) inAedesmosquitoes, the vectors of arboviruses such as dengue and chikungunya viruses. Previous studies identified three EFVEs fromAe. albopictusand one fromAe. aegypticell lines. However, in-depth characterization of EFVEs in wild-type mosquito populations and individualsin vivohas not been performed. We detected the full-length DNA sequence of the previously described EFVEs and their respective transcripts in severalAe. albopictusandAe. aegyptipopulations from geographically distinct areas. However, EFVE-derived proteins were not detected by mass spectrometry. Using deep sequencing, we detected the production of piRNA-like small RNAs in antisense orientation, targeting the EFVEs and their flanking regionsin vivo. The EFVEs were integrated in repetitive regions of the mosquito genomes, and their flanking sequences varied among mosquito populations from different geographical regions. We bioinformatically predicted several new EFVEs from a VietnameseAe. albopictuspopulation and observed variation in the occurrence of those elements among mosquito populations. Phylogenetic analysis of anAe. aegyptiEFVE suggested that it integrated prior to the global expansion of the species and subsequently diverged among and within populations. Together, this study revealed substantial structural and nucleotide diversity of flaviviral integrations inAedesgenomes. Unraveling this diversity will help to elucidate the potential biological function of these EFVEs.ImportanceEndogenous viral elements (EVEs) are whole or partial viral sequences integrated in host genomes. Interestingly, some EVEs have important functions for host fitness and antiviral defense. Because mosquitoes also have EVEs in their genomes, we decided to thoroughly characterized them to lay the foundation of the potential use of these EVEs to manipulate the mosquito antiviral response. Here, we focused on EVEs related to theFlavivirusgenus, to which dengue and Zika viruses belong, inAedesmosquito individuals from geographically distinct areas. We showed the existencein vivoof flaviviral EVEs previously identified in mosquito cell lines and we detected new ones. We showed that EVEs have evolved differently in each mosquito population. They produced transcripts and small RNAs, but not proteins, suggesting a function at the RNA level. Our study uncovers the diverse repertoire of flaviviral EVEs inAedesmosquito populations and suggests a role in the host antiviral system.
Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3
Authors: Kimura Hana , Arasaki Kohei , Ohsaki Yuki , Fujimoto Toyoshi , Tagaya Mitsuo 
Published: April 2017

AbstractLipid droplets (LDs) are ubiquitous organelles that contain neutral lipids and are surrounded by a phospholipid monolayer. How proteins specifically localize to the phospholipid monolayer of the LD surface has been a matter of extensive investigations. Here we show that syntaxin 17 participates in LD biogenesis by regulating the distribution of acyl-CoA synthetase 3 (ACSL3), a key enzyme for LD biogenesis that redistributes from the endoplasmic reticulum to LDs during LD formation. Time course experiments revealed that syntaxin 17 binds to ACSL3 in the initial stage of LD formation, and that ACSL3 is released as a consequence of competitive binding of SNAP23 to syntaxin 17 in the maturation stage. We propose a model in which ACSL3 redistributes from the endoplasmic reticulum to LDs through association with syntaxin 17 and SNAP23-mediated dissociation from syntaxin 17. We also provide evidence that lipid raft-like structures are important for LD formation and SNAREs-ACSL3 interactions.
Genome-wide association study and genomic predictions for resistance against Piscirickettsia salmonis in coho salmon (Oncorhynchus kisutch) using ddRAD sequencing
Authors: Barría Agustín , Christensen Kris A. , Correa Katharina , Jedlicki Ana , Lhorente Jean P. , Davidson William S. , Yáñez José M. 
Published: April 2017

ABSTRACTPiscirickettsia salmonis is one of the main infectious diseases affecting coho salmon (Oncorhynchus kisutch) farming. Current treatments have been ineffective for the control of the disease. Genetic improvement for P. salmonis resistance has been proposed as a feasible alternative for the control of this infectious disease in farmed fish. Genotyping by sequencing (GBS) strategies allow genotyping hundreds of individuals with thousands of single nucleotide polymorphisms (SNPs), which can be used to perform genome wide association studies (GWAS) and predict genetic values using genome-wide information. We used double-digest restriction-site associated DNA (ddRAD) sequencing to dissect the genetic architecture of resistance against P. salmonis in a farmed coho salmon population and identify molecular markers associated with the trait. We also evaluated genomic selection (GS) models in order to determine the potential to accelerate the genetic improvement of this trait by means of using genome-wide molecular information. 764 individuals from 33 full-sib families (17 highly resistant and 16 highly susceptible) which were experimentally challenged against P. salmonis were sequenced using ddRAD sequencing. A total of 4,174 SNP markers were identified in the population. These markers were used to perform a GWAS and testing genomic selection models. One SNP related with iron availability was genome-wide significantly associated with resistance to P. salmonis defined as day of death. Genomic selection models showed similar accuracies and predictive abilities than traditional pedigree-based best linear unbiased prediction (PBLUP) method.
Lipopolysaccharide-binding protein (LBP) can reverse the amyloid state of fibrin seen or induced in Parkinson’s disease: implications for its aetiology
Authors: Pretorius Etheresia , Mbotwe Sthembile , Kell Douglas B. 
Published: April 2017

AbstractThe thrombin-induced polymerisation of fibrinogen to form fibrin is well established as a late stage of blood clotting. In recent work, we showed that the presence of tiny amounts of bacterial lipopolysaccharide (LPS) could cause these clots to adopt an amyloid form, that could be observed via scanning electron microscopy (SEM) or via the fluorescence of thioflavin-T. This could be prevented by the prior addition of lipopolysaccharide-binding protein (LBP). We had also observed by SEM this unusual clotting in the blood of patients with Parkinson’s disease (PD). We here show that this too can be prevented by LBP, thereby implicating such inflammatory microbial cell wall products in the aetiology of the disease. This may lead to novel treatment strategies in PD designed to target microbes and their products.
Forward looking vs backward looking
Authors: Gao Yanyan , Sun Jun , Zhou Qin 
Published: April 2017

Purpose
The purpose of this paper is to estimate the effectiveness of the credit evaluation system using the borrowing data from China’s leading P2P lending platform, Renrendai.com.

Design/methodology/approach
The current credit valuation systems are classified into the forward-looking mechanism, which judges the borrowers’ credit levels based on their uploaded information, and the backward-looking mechanism, which judges the borrowers’ credit levels based on their historical repayment performance. Probit models and Tobit models are used to examine the effectiveness of credit evaluation mechanisms.

Findings
The results show that only the “hard” information reflecting borrowers’ credit ability can explain the default risk on the platform under the forward-looking credit evaluation mechanism. The backward-looking credit evaluation mechanism (BCEM) based on the repeated borrowings produces both promise-enhancing and “fishing” incentives and thus fails to explain the default risk, and weakens the effectiveness of forward-looking credit indicators in explaining the default risk because it encourages borrowers to invest in forging forward-looking credit indicators. Additional information such as the interest rate and the repayment periods reveals borrowers’ credit and thus can also be used as a predictor of borrowers’ default risk.

Practical implications
The findings suggest that current ex ante screening based on the information collected from the borrowers or repeated borrowings is inadequate to control the default risk in P2P lending markets and thus needs be improved. Ex post monitoring and sharing on defaulter’s information should be strengthened to increase the default cost and thus to deter potential bad borrowers.

Originality/value
To the authors’ knowledge, this is the first paper classifying the credit evaluation system in online P2P lending market into the forward-looking type and the backward-looking type, which is important since they provide different incentives to borrowers. The paper also investigates and provides evidence on the promise-enhancing and “fishing” incentives of BCEMs.
Energy-led refurbishment of non-domestic buildings: ranking measures by attributes
Authors: Strachan Megan E. , Banfill Phil FG 
Published: April 2017

Purpose
In the context of the energy-led refurbishment of traditionally constructed non-domestic buildings, the purpose of this study was to identify the attributes that professionals consider to be important in the selection of energy performance improvement measures (EPIMs) and to establish their relative importance.

Design/methodology/approach
A questionnaire-based Delphi exercise was carried out to draw up a set of attributes agreed among a small panel of experts. Subsequently, a paired comparison questionnaire was completed by the experts to establish the relative importance ascribed to the attributes.

Findings
In total, 22 assessment attributes, relevant to different stages in the building’s life cycle, were agreed as important by the expert panel. Measures fell into one of three broad groups, expressed on a weighting scale of 0-100, such that the sum of the weights of all 22 measures was 100. Measures of relatively high importance (7-9 per cent) included capital cost, potential energy and carbon savings, financial payback and impact on the building’s vapor permeability. Measures of medium importance (4-5 per cent) were impact on internal air movement, loss of significant original building fabric, impact on internal layout, appearance and occupant comfort, environmental impact and availability of grants or subsidies. Eight further measures were ranked of low importance (2-3 per cent).

Originality/value
This paper is the first attempt to draw up a ranking order of the attributes of EPIMs applicable to non-domestic buildings. It confirms that more attributes must be considered by professionals with decision-making responsibility than merely cost, energy performance and payback on investment and suggests that policy instruments targeting or incentivizing a single or a restricted range of attributes risk failure to deliver the desired improvements.
Advancement of behavioral energy interventions in commercial buildings
Authors: Kosonen Heta Karoliina , Kim Amy Ahim 
Published: April 2017

Purpose
The purpose of this paper is to identify opportunities, barriers and guidelines for future research in behavioral energy interventions in commercial buildings.

Design/methodology/approach
The study methodology includes a three-step screening protocol with a collection of prior publications, clustering of related studies and results and analysis of the findings of the prior studies.

Findings
The review showed that commercial energy interventions were generally successful at impacting occupant energy consumption. Most energy savings were obtained by applying comparative feedback and energy competition strategies, but the lack of long-term effect measurements prevents drawing conclusions regarding their long-term effectiveness. The authors suggest that future studies should explore the impacts that occupant characteristics, environment and community and intervention implementation have on the success of the energy intervention, and integrate these findings into the intervention design. In addition, the authors call for more discussion on the feasibility issues that researchers, policymakers and educators face when implementing these energy interventions to streamline sustainability efforts in the future.

Originality/value
Research on assessing the effectiveness of occupant behavior interventions has increased considerably over the past decade. This review includes a structured analysis of prior studies of behavioral energy interventions in commercial buildings and encompasses studies conducted between 2005 and 2015. The review is unique in that it focuses on comparing empirical studies that quantified measured energy savings.
Life-cycle cost analysis (LCCA): a comparison of commercial flooring
Authors: Harris Debra , Fitzgerald Lori 
Published: April 2017

Purpose
The business case for facility expenditures is grounded in the knowledge that life-cycle economics is significant to the continued viability of the facility. The aim of this study is to develop an algorithm for life-cycle cost analysis (LCCA) and evaluate flooring products to inform decision makers about the long-term cost of ownership.

Design/methodology/approach
The protocol for executing an LCCA is defined by the National Institute of Standards and Technology, including defining the problem, identifying feasible alternatives and establishing common assumptions and parameters, as well as acquiring financial information. Data were provided by an independent third-party source.

Findings
The results of this study are twofold: assess functionally equivalent flooring alternatives to determine the best financial value and develop a replicable protocol and algorithm for LCCA. The study found that modular carpet was the best financial solution. As a tool for decision makers, this LCCA informs asset management about the long-term cost of ownership, providing a protocol for making practical, informed decisions for the lowest cost solution for functionally equivalent alternatives.

Research limitations/implications
Projecting LCCA beyond 15 years may have limited value based on potential changes in the financial climate. Further research should focus on the implications of changes in the discount rate over time and testing the algorithm on other building systems.

Practical implications
Maintenance costs are considerable when compared to initial cost of flooring. Equipment costs have a significant impact on long-term cost of ownership. Using LCCA to inform specifications and to determine the best solution for a building system such as flooring provides an evidence-based process for building design and facility management.

Social implications
Life-cycle costs have a significant impact on the financial health of an organization. Using LCCA to make informed decisions about facility design and specifications may contribute to increased financial stability and resources to benefit the organization’s long term goals.

Originality/value
This study contributes an algorithm instrument for buildings and building systems. The flooring tested with this protocol provides evidence to inform flooring selection based on lowest cost while considering other factors that inform appropriate selection of flooring materials.
Generation difference in hiring financial planners in Malaysia
Authors: Safari Meysam , Mansori Shaheen , Sesaiah Stephen 
Published: April 2017

Purpose
The purpose of this paper is to document a gap between generation X and Y’s behavior toward decision making for hiring a professional financial planner in context of an emerging country.

Design/methodology/approach
This research is based on a public survey in Malaysia on the effect of five major contributing factors (namely, awareness, acceptability, affordability, accessibility and assurance) on the decision to hire a professional financial planner. The study further shed light into the difference among the influential factors among generation X and Y.

Findings
Although awareness, acceptability, affordability and assurance have demonstrated significant effect on decision making in general, their impact varies among different age groups. Results of moderation tests on the role of age suggest that for Gen X, the determinant factor is only their acceptability of the financial planning service. However, awareness, affordability, acceptability and assurance are critical factors for Gen Y respondents. In contrast to Gen Y, the Gen X respondents tend to have more awareness toward their needs for financial planning; they have gained enough experience to assess the credibility of the planner and test their assurance; and have higher earnings to afford the financial planners services.

Originality/value
Findings of this study are novel as it provide first hand picture from an emerging market in South-East Asia. Moreover, the study documents generation gap in financial decision making process.