bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Potential of Nanotechnology in Anti-Cancer Drug to Regulate Nrf2 Signaling for Cancer Therapeutic Purposes
Authors: Yari Abolfazl , Vazifeshenas-Darmiyan Khadijeh , Bakhshi Haniye , Kabiri-rad Hamid , Torabi Mohammadamin , Shabib Forouzan , Samarghandian Saeed , Farkhondeh Tahereh 
Published: October 2024

Abstract:
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a regulator of the cellular
antioxidant defense system that plays an important role in reducing the risk of various
pathophysiological conditions, including cancer. Targeting Nrf2 presents an attractive
therapeutic approach to overcome these challenges and improve cancer treatment outcomes.
Nanoparticles, with their unique physicochemical properties, offer several advantages
over conventional therapies for targeting Nrf2. These include enhanced stability,
improved permeability and retention effect, and precise targeting capabilities. Moreover,
delivery systems based on nanotechnology have shown promise in overcoming the limitations
of conventional cancer therapies, including ineffective precision targeting and
momentous complications. The therapeutic efficacy of Nrf2 inhibitors may be enhanced
by using nanoparticles for specific drug targeting and deeper tissue penetration. This involves
optimizing nanoparticle formulations, understanding their interactions with the biological
environment, and ensuring their safety and biocompatibility. Effective nanoparticle
formulations are being developed to transport Nrf2 inhibitors, which can significantly
improve treatment outcomes and address the limitations of conventional cancer therapies.
Further studies are needed to explore the potential of nanotechnology in targeting
Nrf2 for cancer therapeutic purposes.
Elucidating the Role of Autophagy-related Genes in Polycystic Ovary Syndrome: Implications for Diagnostic Models and Immune Response
Regulation
Authors: Li Nan , Yu Kai , Huang Delun , Guo Hua , Zhu Xuehong , Lin Zhong 
Published: October 2024

Background:
Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder
that negatively affects female reproductive capacity. Although the association between
autophagy and PCOS is known, there are few detailed studies on the association
between autophagy-related genes and PCOS.

Methods:
Publicly available gene expression datasets (GSE102293, GSE138518,
GSE34526, GSE114419, GSE137684, GSE155489) were used in a comprehensive analysis
to identify a role for autophagy in PCOS. Batch effects were mitigated using the sva
package, followed by WGCNA (weighted gene correlation network analysis) and ss-
GSEA (single sample gene set enrichment analysis) to identify autophagy-related genes.
Recursive feature elimination (RFE) and LASSO COX methods were used to identify
important hub genes, and their correlation with immune cell activity was assessed using
ssGSEA and Pearson correlation analysis.

Results:
High autophagy scores were observed in PCOS samples, and the dark green
gene module with the highest autophagy correlation was identified. The differential analysis
identified a total of 169 up-regulated genes versus 2 down-regulated genes in the
PCOS samples, which were intersected by taking the intersection with the deep green
module genes and resulted in 121 key genes. Subsequently, 6 hub genes (MMP25, CSF3R,
SLPI, MMP9, CLEC4E, and SIGLEC10) were further identified based on RFE
and LASSO algorithms. Diagnostic efficacy based on ROC curves showed six autophagy-
associated hub genes with AUC values as high as 0.959 and 0.896 in the training and
validation sets, respectively. Finally, we observed that these hub genes are strongly associated
with immune function, especially chronic inflammation and aberrant immune activation
pathways.

Conclusion:
In this study, we identified autophagy genes closely related to PCOS and
constructed a gene model with high diagnostic accuracy. These findings not only provided
potential new biomarkers for the diagnosis of PCOS but also revealed the key role of
autophagy in the pathogenesis of PCOS.
Design, In Silico, and In vitro Evaluation of Polymer-Based Drug Conjugates Incorporated with Derivative of Cinnamic Acid, Zidovudine, and 4-Aminosalicylic Acid against Pseudo-HIV-1
Authors: Naki T. , Matshe W.M.R. , Obisesan O. , Balogun M.O. , Oselusi S.O. , Ray S.S. , Aderibigbe B.A. 
Published: October 2024

Background:
The incorporation of anti-HIV drugs into polymer to form polymer-drug
conjugates has been reported to result in improved therapeutic activity. Zidovudine, an anti-HIV
drug, was explored alone and in combination with known drug molecules using polyamidoaminebased
carriers.

Objective:
Polymer-drug conjugates incorporated with zidovudine, cinnamic acid, and 4-aminosalicylic
acid were prepared and evaluated for their potential efficacy in vitro against pseudo-
HIV-1.

Methods:
Aqueous Michael addition polymerization reaction was employed to prepare the conjugates.
The conjugates were incorporated with zidovudine, cinnamic acid, and 4-aminosalicylic
acid. They were characterized by SEM/EDX, XRD, FTIR, NMR, LC-MS, particle size analysis,
in vitro analysis, computational studies, and in silico toxicity predictions.

Results:
The conjugates displayed spherically shaped morphology. The in vitro findings showed
that polymer-drug conjugates, T15 and T16, with a single drug were effective against pseudo-
HIV-1 at high concentrations of 111.11 and 333.33 μg/mL, respectively. The molecular docking
studies confirmed the in vitro results. The Swiss ADME, ProTox-II, and GUSAR (General Unrestricted
Structure-Activity Relationships) revealed that these compounds are promising antiviral
compounds.

Conclusion:
The prepared polymer-drug conjugates with a single drug showed promising effects
against the Pseudo-HIV-1, and the conjugates displayed features that make them potential anti-
HIV therapeutics that require further studies.
Preparation of New Liposomal Daunorubicin and Evaluation of its Anti-colorectal Cancer in HCT116 Cells
Authors: Mahdavi Faezeh , Rahiminezhad Arezo , Kami Aref Jafarpoor , Arasteh Amir , Ebrahimi Mehdi 
Published: October 2024

Background:
Colorectal cancer (CRC) is the third most common cause of cancerrelated
deaths worldwide. To develop more effective anti-CRC drugs, this research evaluated the
impact of synthesized liposomal daunorubicin on HTC116 colon cancer cell line.

Methods:
Liposomal daunorubicin (LDNR) was synthesized by the thin layer hydration method
and size was determined by dynamic light diffraction (DLS). MTT assay was used to determine
the cytotoxicity and IC50 of LDNR against the HCT116 CRC cell line. Relative mRNA expression
of the NF-κB gene and apoptosis were evaluated in 24 hours treatments of HCT116 cells by
qRT-PCR and flow cytometry, respectively.

Results:
The hydrodynamic diameter of liposomes containing daunorubicin (DNR) was determined
25.2 nm. MTT assay showed a 38% decrease in HCT116 cells viability after 24-hour
treatment with the DNR (0.5 μM). The lowest (0.125 μM) and highest (2 μM) dose of LDNR
showed 20.4% and 71.6% cytotoxicity, respectively. LDNR showed dose-dependent cytotoxicity
with the IC50 of 0.87 μM. The phase contrast microscope evaluation confirmed the LDNR cytotoxicity.
The DNR and LDNR (0.87 μM) decreased relative mRNA levels of NF-κB 63% (P=
0.023) and 99.6% (P=0.003), respectively. The percentage of apoptotic cells in the DNR and
LDNR increased by 27.1% (P<0.0001) and 49.7% (P<0.0001), respectively.

Conclusion:
DNR increases the rate of apoptosis by decreasing the NF-κB gene expression in
HCT116 cells. These effects are intensified in the liposomal form. Therefore, the LDNR produced
in this research can be considered in the treatment of CRC.
Amine-tethered DMAP Cation Catalysts: An Efficient Organocatalytic System for the Chemical Fixation of CO2 to Five-Membered Cyclic
Authors: Dewi Rita Sharmali , Khaligh Nader Ghaffari 
Published: October 2024

Introduction:
In this study, three amine-tethered DMAP cation bromide catalysts were
prepared using two different mole ratios of 4-dimethylpyridine (DMAP) and 3-bromopropylamine
hydrobromide (BPA·HBr) in two different solvents, namely, acetonitrile and ethanol. Then, prepared
catalysts were employed for CO2 cycloaddition with styrene and propylene epoxides under
metal- and solvent-free conditions.

Method:
The impact of mole ratio and solvent selection to prepare the designed product using a
simple and cost-effective procedure was demonstrated systematically and was discussed in detail.
Moreover, the influence of amine-tethered DMAP cation catalyst structures and reaction conditions
on the cycloaddition was investigated, and the CO2 conversion proceeded smoothly at 80°C and 0.2
MPa for the synthesis of cyclic carbonates in good to excellent yields and high selectivity.

Results:
The current protocol could be a promising process at an industrial scale due to the high
recyclability of the catalyst (10 cycles).

Conclusion:
In summary, three catalytic systems bearing amine-tethered DMAP cation have been
investigated for the chemical fixation of CO2 into five-membered cyclic carbonates at mild conditions.
Ethical and Methodological Considerations in LGBTQ-focused Online Ethnography: A Proposal of a Systematic Review
Authors: Wang Shengang , Wang Ning-Chiao , Lee Wan-Chen 
Published: October 2024

Lesbian, gay, bisexual, transgender, and queer (LGBTQ) people constitute a vulnerable population that requires research about their needs, representations, and challenges. Among the variant research methods, ethnography is particularly effective in understanding participants and their lived experience. The development of information and communication technologies has transformed traditional ethnography research and contributed to the emergence of online ethnography research that holds potential for studying vulnerable populations (e.g., LGBTQ people) and relevant topics.
We propose a systematic review to explore how researchers have considered and addressed ethical and methodological issues in LGBTQ-focused online ethnography research. We used the core collection of the Web of Science to search for relevant literature. We crafted the search query based on studies of LGBTQ populations, online ethnography studies, and other data sources like the Homosaurus vocabulary. Two groups of search terms were compiled and used in combination, including population-related terms (e.g., “queer”, “LGB*”, “GLB*”, and “sexual minorit*”) and method-related terms (e.g., “online ethnograph*”, “netnograph*”, “digital ethnograph*”, and “virtual ethnograph*”). In this review, we focus on empirical journal articles using any type or variant of online ethnography to investigate LGBTQ-focused topics. The eligible articles may include LGBTQ people as participants or stakeholders (e.g., LGBTQ staff), or analyze LGBTQ-related content (e.g., YouTube videos). Most importantly, we will analyze the ethical concerns (e.g., informed consent, power imbalance, cultural sensitivity, and beneficence and justice) and methodological aspects (e.g., research design, sampling, recruitment, data collection, data analysis, and roles of LGBTQ people) of these studies.
READCON: A Curriculum for Library Readiness, Advocacy, and Community Empowerment During Challenging Conditions
Authors: Santos Green Lucy , Dawkins April , Byrd-Fort Valerie 
Published: October 2024

This panel introduces the READCON curriculum: Library Readiness, Advocacy, and Community Empowerment During Challenging Conditions The curriculum equips library workers with soft skills to develop relationships with their communities, partnering more effectively for advocacy. Building on the highly successful Get Ready Stay Ready Community Action Toolkit (GRSR), this curriculum builds foundational skills for library workers to engage in constructive dialogue on potentially divisive aspects of library programming, service, and material challenges. READCON takes Get Ready Stay Ready a step further by providing library workers with the soft skills needed to encourage community members to use the action steps included in the GRSR toolkit. Both resources are highly adaptable, multi-lingual, open-source learning tools developed by experts in academic, public, and school librarianship, education and family law, crisis communications, community advocacy and relations, and youth leadership.
Moisture Control Using A Low- Cost Experimental Approach For A Vertical Agricultural Indoor Module
Authors: Borrero Henry , Montenegro Danna , Pabon Nicolás , Duran Laura , Vidal Rafael , Tiuso Juan 
Published: October 2024

This paper describes an indoor vertical farming low-cost approach undertaken under experimental learning considerations. A structure measuring approximately one meter in height, 0.27 meters in width, and 0.38 meters in depth was constructed to examine the growth of arugula seeds. We equipped our vertical farming structure with emitters of artificial light in order to facilitate the photosynthesis of arugula plants that were planted in trays in a building without sufficient natural light. Further, soil moisture in the trays was monitored so that electromechanical water pumps were activated whenever a high level of soil dryness was detected. As a control unit, an ESP32 microcontroller was used. It was programmed to read sensors and to apply control signals as required. Additionally, ESP32 was configured to send data from sensors to an Internet of Things (IoT) web page, enabling us to monitor the system remotely. BIPES (Block-based Integrated Platform for Embedded Systems) is an intuitive programming tool that is available to the general

public through the internet, which was used for programming the ESP32. As a final aspect, researchers at the Universidad Distrital Francisco José de Caldas, the Corporación Universitaria Minuto de Dios, in Colombia, and the Universidade Federal of Sao Carlos, in Brazil, became interested in vertical farming as a topic to be involved in, leading to the development of a first approach which is presented in this paper.
Dogs receiving cyclooxygenase-2–sparing nonsteroidal anti-inflammatory drugs and/or nonphysiologic steroids are at risk of severe gastrointestinal ulceration
Authors: Hillier Townes N. , Watt Meghan M. , Grimes Janet A. , Berg Alexia N. , Heinz Justin A. , Dickerson Vanna M. 
Published: October 2024

Abstract

OBJECTIVE
To report the incidence and characteristics of gastrointestinal ulceration lesions in dogs receiving an NSAID and/or corticosteroid.

ANIMALS
33 dogs.

CLINICAL PResentation
Medical records of dogs with gastrointestinal ulceration receiving NSAIDs and/or corticosteroids within 30 days of diagnosis between January 2012 and July 2022 at multiple referral institutions were reviewed. Diagnosis was confirmed via endoscopy, surgery, or necropsy. Clinical data were collected from the medical record, including the dose and reason for administration of NSAIDs or steroids.

RESULTS
Dogs received a single NSAID (n = 22, most commonly carprofen [9], meloxicam [4], and deracoxib [3]), 2 NSAIDs (5), a single steroid (5: prednisolone [2], prednisone [2], or dexamethasone SP [1]), or an NSAID and steroid (1). Eleven dogs receiving a single cyclooxygenase (COX)-2–sparing NSAID at an appropriate dose had ulcerations. All dogs receiving 2 NSAIDs concurrently experienced full-thickness perforation (5 of 5). The most common ulcer locations were duodenum (n = 18) and pylorus (11). Abdominal ultrasound correctly identified the site of ulceration in 5 of 24 dogs.

CLINICAL RELEVANCE
Dogs receiving COX-2 sparing NSAIDs at recommended doses are at risk of severe GI ulceration. Carprofen was the most common NSAID resulting in ulceration; however, it is one of the most prescribed NSAIDs. Adding another NSAID and steroid could increase this risk. Careful monitoring is crucial for dogs on NSAIDs, regardless of duration.
Rootstock affects phytotechnical attributes, gas exchange, and carbohydrate accumulation in mango scion
Authors: Santos Adriana da Silva , Gomes Fernando Antonio Lima , Silva Luan dos Santos , Neto Vespasiano Borges de Paiva , Silva Maiara Tatiane Lima , Silva Ana Carolina Rodrigues da , Cavalcante Ítalo Herbert Lucena 
Published: October 2024

ABSTRACT
The market demand for grafted mango seedlings is increasing because they provide uniformity and precocity for orchards and gain in productive performance. However, studies on suitable rootstocks for mango seedling production of the main mango cultivars of economic interest in the Sao Francisco Valley, Brazil, are incipient. In this context, the objective of the present study was to evaluate growth, gas exchange, and carbohydrate accumulation in the 'Palmer', 'Tommy Atkins', 'Kent', and 'Keitt' mango scions grafted onto polyembryonic rootstocks ('Capucho', 'Coquinho', and 'Espada'). The experiment was carried out under nursery conditions, with 50% shading. A randomized block experimental design was used, in a 3 × 4 factorial arrangement, consisting of three mango rootstocks ('Espada', 'Capucho', and 'Coquinho') and four mango scion cultivars ('Palmer', 'Keitt', 'Kent', and 'Tommy Atkins'), with five replications and five plants per plot. Graft success was evaluated 28 days after grafting (DAG) to determine the effects of the rootstocks on each scion. Growth, photosynthetic parameters, and total soluble carbohydrate (TSC) and starch content were evaluated 227 DAG. The rootstocks used affected the growth, photosynthetic pigments, contents of TSC and starch, and gas exchange of the mango scions. The higher graft success percentages were found for the cultivars 'Keitt', 'Palmer', and 'Kent' grafted on 'Espada' rootstocks. The cultivar 'Tommy Atkins' showed the highest graft success percentages when grafted on 'Espada' and 'Capucho' rootstocks. 'Capucho' rootstocks showed higher performance for most of the analysed variables, mainly when using 'Palmer' and 'Tommy Atkins' cultivars as scions.
The Safe Insulation from the Inside as a Solution to the Energy and Climate Crisis
Authors: Bogdanovs Mihails , Blumberga Andra , Freimanis Ritvars 
Published: October 2024

Abstract
During the time when the European Union is facing an energy crisis, it is essential to understand ways to overcome it more easily. As one of the consuming sectors, buildings play a crucial role in improving energy efficiency. Therefore, one of the ways to fight with energy crisis and high energy consumption in buildings is insulation from the inside. This study assesses the hygrothermal performance of masonry walls with 9 interior insulation systems (mineral wool with vapor barrier, XPS, PIR, cork, expanded cork, aerogel blanket, wood fibre plates, perlite board) exposed to different external conditions in the climate chambers. Masonry walls were tested in a steady cycle with cold box temperature 18 °C and 40 % RH), a dynamic cycle that follows daily fluctuations, a dynamic cycle with rain. Also, an identical simulation of the hygrothermal process was carried out in the DELPHIN software to compare results of both testing methods. The temperature between the thermal insulation layer and the brick wall in all thermal insulation systems is approximately 7-8 hours behind the temperature of the outdoor climatic chamber. A dynamic cycle with rain simulation has a significant impact on the hygrothermal behaviour of thermal insulation systems. The study provides valuable data on hygrothermal processes in different wall insulation systems from the inside.
Distribution, density and trends of the Eurasian eagle owl (Bubo bubo) population breeding in March-Thaya floodplain forests: impact of owlets on sustainability of natural nests
Authors: Svetlíková Soňa , Svetlík Ján , Škorpíková Vlasta , Matušík Hynek , Štěpánek Pavel , Horal David , Zuna-Kratky Thomas 
Published: October 2024

Abstract
In this study, we investigated the Eurasian eagle owl (Bubo bubo) breeding in lowland forests in the trans-border area between western Slovakia, eastern Austria, and southern Moravia. The research provides new information on the reuse of nests by eagle owls and presents initial insights into population density and trends of eagle owls in the March-Thaya floodplains. Our 19-year monitoring has shown that the eagle owl has become a widespread breeder over the study area, with an increasing population trend. A total of 151 breeding attempts by eagle owls have been identified, occupying 82 natural nests (originally built by at least nine species of birds) and 12 artificial nests. With an average of 6.2 active nests per 100 km2 and a maximum of 17 active nests found in 2021 (~10.6 pairs per 100 km2), our findings represent one of the highest eagle owl breeding densities found, especially in comparison with core populations nesting in the mountains (the Carpathians, north-eastern Alps and the Bohemian Massif). Regarding the dynamics of nest reuse, our results reveal that only a third of nests used by eagle owls were reused by other raptors or storks (Ciconia sp.). Almost 50% of the natural nests in which eagle owl bred, subsequently disintegrated after the owlets had fledged. Lastly, black stork (Ciconia nigra) nests re-used by eagle owls were twice as likely to have disintegrated after the owlets had fledged than nests built by other bird species. Our results suggest that black stork nests in the March and Thaya floodplain forests are most susceptible to destruction.
Bird crime and the assessment of risk areas in Slovakia
Authors: Veselovský Tomáš , Maderič Boris , Chavko Jozef , Svetlík Ján 
Published: October 2024

Abstract
Bird crime is a serious problem in many countries around the world. This study focuses on the situation in Slovakia, summarising data on bird crime in the country between 2016 and 2022. Over the course of that period, 92 cases were recorded in 27 districts involving 33 animal species. At least 249 protected birds and 78 mammals fell victim to illegal activities, and 61 poisoned baits were discovered. Social value is the monetary value which state institutions use to quantify the damage incurred to wildlife. Slovak law determines whether an illegal act is an offence or a criminal act based on the value of the incurred damage. The total social value of all of the protected animals as a result of illegal activities was estimated at € 696,250. Intentional poisoning, typically using carbofuran, was the most common of illegal act identified in the recorded cases, followed by shooting and trapping. An analysis of the recorded cases of bird crime was also used to assess the risk in each of Slovakia’s districts in relation to landscape structure variables. The percentage share of arable land was found to be a reliable predictor of bird crime, regardless of whether the birds had been poisoned or shot. These findings can help to develop better preventive inspections to detect bird crime incidents. We also recommended several management measures to help tackle bird crime more quickly and effectively.