bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of rifampicin in human plasma by high‐performance liquid chromatography coupled with ultraviolet detection after automatized solid–liquid extraction
Authors: Louveau B. , Fernandez C. , Zahr N. , Sauvageon‐Martre H. , Maslanka P. , Faure P. , Mourah S. , Goldwirt L. 
Published: June 2016

AbstractA precise and accurate high‐performance liquid chromatography (HPLC) quantification method of rifampicin in human plasma was developed and validated using ultraviolet detection after an automatized solid‐phase extraction. The method was validated with respect to selectivity, extraction recovery, linearity, intra‐ and inter‐day precision, accuracy, lower limit of quantification and stability. Chromatographic separation was performed on a Chromolith RP8 column using a mixture of 0.05 m acetate buffer pH 5.7–acetonitrile (35:65, v/v) as mobile phase. The compounds were detected at a wavelength of 335 nm with a lower limit of quantification of 0.05 mg/L in human plasma. Retention times for rifampicin and 6,7‐dimethyl‐2,3‐di(2‐pyridyl) quinoxaline used as internal standard were respectively 3.77 and 4.81 min. This robust and exact method was successfully applied in routine for therapeutic drug monitoring in patients treated with rifampicin.
A transient increase and subsequent sharp decrease of chemo‐refractory liver‐metastasized uterine cervical small cell carcinoma to autologous formalin‐fixed tumor vaccine plus anti‐PD‐1 antibody
Authors: Miyoshi Tatsu , Kataoka Tatsuji , Asahi Atsuko , Maruyama Takashi , Okada Rika , Uemae Yoji , Ohno Tadao 
Published: June 2016

Key Clinical MessageUterine cervical small cell carcinoma is rare and aggressive with no standardized therapy. A patient bearing the advanced chemo‐refractory carcinoma, treated with a tumor vaccine combined with 1 mg/kg of pembrolizumab, showed a transient increase and subsequent sharp decrease of the liver‐metastasized lesion to less than half its maximum diameter.
Numerical study on heat transfer and pressure drop characteristics of R410A condensation in horizontal circular mini/micro‐tubes
Authors: Zhang Jingzhi , Li Wei 
Published: June 2016

AbstractHeat transfer and pressure drop characteristics of condensation for R410A inside horizontal circular tubes with inner diameters ranging from 0.25–4 mm were investigated numerically. The effects of mass flux, vapour quality, tube diameters, and gravity on heat transfer and pressure drop characteristics were discussed. Liquid‐vapour interfaces and stream‐traces were also presented to give a better understanding of these effects on the condensing flow. Numerical heat transfer coefficients and pressure gradients fitted well with the empirical correlations. The results indicate that local heat transfer coefficients and pressure drop increase with increasing mass flux, vapour quality, and with decreasing tube diameters. A thin liquid film is formed at the upper part of the tube in mini‐channels, while a symmetrical annular flow is observed in microchannels. A vortex with its core lying at the bottom of the tube is formed in the tubes with an inner diameter of 4 mm, while the stream‐traces start from the z‐axis and point to the liquid‐vapour interface when dh is 0.25 mm. The gravity effect on heat transfer is important in larger tubes at lower vapour quality, where stratified flow may occur.
New Disaccharide‐Based Ether Lipids as SK3 Ion Channel Inhibitors
Authors: Berthe Wilfried , Sevrain Charlotte M. , Chantôme Aurélie , Bouchet Ana Maria , Gueguinou Maxime , Fourbon Yann , Potier‐Cartereau Marie , Haelters Jean‐Pierre , Couthon‐Gourvès Hélène , Vandier Christophe , Jaffrès Paul‐Alain 
Published: June 2016

AbstractThe SK3 potassium channel is involved in the development of bone metastasis and in the settlement of cancer cells in Ca2+‐rich environments. Ohmline, which is a lactose‐based glycero‐ether lipid, is a lead compound that decreases SK3 channel activity and consequently limits the migration of SK3‐expressing cells. Herein we report the synthesis of three new ohmline analogues in which the connection of the disaccharide moieties (1→6 versus 1→4) and the stereochemistry of the glycosyl linkage was studied. Compound 2 [3‐(hexadecyloxy)‐2‐methoxypropyl‐6‐O‐α‐d‐glucopyranosyl‐β‐d‐galactopyranoside], which possesses an α‐glucopyranosyl‐(1→6)‐β‐galactopyranosyl moiety, was found to decrease SK3 current amplitude (70 % inhibition at 10 μm), displace SK3 protein outside caveolae, and decrease constitutive Ca2+ entry (50 % inhibition at 300 nm) and SK3‐dependent cell migration (30 % at 300 nm) at a level close to that of the benchmark compound ohmline. Compound 2, which decreases the activity of SK3 channel (but not SK2 channel), is a new drug candidate to reduce cancer cell migration and to prevent bone metastasis.
Widely linear channel estimation for LD‐STBC MB‐OFDM UWB systems
Authors: Hajjaj Moufida , Chainbi Walid , Bouallegue Ridha 
Published: June 2016

SummaryThe combination of Linear Dispersion Space‐Time Block Codes (LD‐STBCs) and Multiband Orthogonal Frequency Division Multiplexing Ultra‐WideBand (MB‐OFDM UWB) technologies has been proposed in the literature to improve the error performance and data rate. Recently, it has been shown that the LD codes allow the repetitions of data symbols conjugates. They cause a transformation of the Multiple‐Input Multiple‐Output (MIMO) channel that requires a Widely Linear (WL) filtering. However, proposed LD‐STBC MB‐OFDM UWB system does not take this issue into account. In this paper, we propose a Widely Linear Minimum Mean Square Error (WLMMSE) channel estimator for LD‐STBC MB‐OFDM UWB systems. The proposed channel estimator proceeds to WL estimation instead of linear one. Via simulation results, we prove that the LD‐STBCs provide significant performance gain for MB‐OFDM UWB system compared to Single‐Input Single‐Output (SISO) configuration. We show also that the proposed WLMMSE channel estimator outperforms the LMMSE one. This performance improvement is observed for both proper and improper signal constellations. Additionally, the computational complexity of our proposal is comparable with that of LMMSE estimator. Copyright © 2016 John Wiley & Sons, Ltd.
Ultrasonographic and cytopathological features of an inflammatory pseudonodule in the thyroid gland
Authors: Jung Soo Jin , Kim Dong Wook 
Published: June 2016

ObjectiveNo previous study has investigated the ultrasonography (US) and cytopathological features of an inflammatory pseudonodule (IFP) associated with underlying thyroiditis. This study aimed to evaluate and identify the characteristic US features of IFP in patients who underwent US‐guided fine‐needle aspiration (US‐FNA).MethodsFrom January 2012 to December 2014, 899 patients underwent US‐FNA for a solid thyroid nodule that was ≥5 mm in diameter, by a single radiologist. Among them, 49 nodules showed lymphocytic thyroiditis on cytological analysis. Based on cytohistopathological and follow‐up US results, 28 IFP cases were included. The same radiologist and a cytopathologist retrospectively analyzed all the US images and the cytology slides, respectively.ResultsThe locations of 28 IFPs were from the right lobe (n = 13), left lobe (n = 13), and isthmus (n = 2). The echogenicity of 28 IFPs was hypoechoic (n = 5) or marked hypoechoic (n = 23). The common US features of IFP were marked hypoechogenicity, no calcification, smooth margin, ovoid shape, and the same vascularity as adjacent parenchyma. However, no IFP cases showed calcification, spiculated margin, taller‐than‐wide shape, and central or peripheral vascularity. The common cytopathological feature of IFP was lymphocyte infiltration, but the intensity of this was variable, with mild, moderate, and severe intensity observed in 8 (28.6%), 11 (39.2%), and 9 (32.1%) of cases, respectively.ConclusionIFP rarely showed malignant US and cytopathological features, except for marked hypoechogenicity, and these findings may be helpful for US and cytopathological diagnoses. Diagn. Cytopathol. 2016;44:725–730. © 2016 Wiley Periodicals, Inc.
TIL 2.0: More effective and predictive T‐cell products by enrichment for defined antigen specificities
Authors: Schober Kilian , Busch Dirk H. 
Published: June 2016

Adoptive transfer of in vitro‐expanded T cells derived from tumor‐infiltrating lymphocytes (TILs) in melanoma patients started the era of tumor immunotherapy three decades ago. The approach has demonstrated remarkable clinical responses in several studies since. Reinfusion of TIL‐derived T cells represents a highly personalized form of immunotherapy, taking into account the enormous interindividual tumor heterogeneity. However, despite its successes, TIL therapy does not lead to objective clinical responses in all cases. It is thus crucial to find out which tumor antigens are particularly valuable targets and to develop strategies to enhance the reactivity of T‐cell products toward them. In this issue of the European Journal of Immunology, Kelderman et al. [Eur. J. Immunol. 2016. 46: 1351–1360] present a platform for the generation of antigen‐specific TIL therapy. Combining recently developed technologies for clinical identification and enrichment of antigen‐specific CD8+ T cells, such as MHC Streptamers and UV‐mediated peptide exchange, the authors could enrich T‐cell populations with defined antigen specificities from melanoma‐derived TILs. This T‐cell product showed higher reactivity against autologous tumor cell lines than bulk TIL‐derived T cells. The novel platform might enable the generation of more effective and predictable TIL‐derived T‐cell products for future clinical applications.
Preparation and characterization of aluminum hypophosphite/reduced graphene oxide hybrid material as a flame retardant additive for PBT
Authors: Qi Yanxia , Wu Weihong , Liu Xiaowei , Qu Hongqiang , Xu Jianzhong 
Published: June 2016

SummaryAluminum hypophosphite/reduced graphene oxide (AHP/RGO) hybrid flame retardant with high thermal stability was successfully prepared by a one‐step method consisting of the simultaneous reduction of graphene oxide and the deposition of AHP on graphene. The as‐prepared sample was characterized by X‐ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, and X‐ray photoelectron spectroscopy. The obtained sample was used as a flame retardant for polybutylene terephthalate, and the flame retardancy of the composites was investigated by a limiting oxygen index test, a UL‐94 test, and cone calorimetry. The results showed that AHP/RGO exhibited improved flame retardancy when compared with bare AHP. The addition of AHP/RGO to polybutylene terephthalate led to a significant reduction in the heat release rate and resulted in excellent anti‐dripping properties for the composites. Copyright © 2016 John Wiley & Sons, Ltd.
Optimal solution error quantification in variational data assimilation involving imperfect models
Authors: Shutyaev V. , Gejadze I. , Vidard A. , Le Dimet F.‐X. 
Published: June 2016

SummaryThe problem of variational data assimilation for a nonlinear evolution model is formulated as an optimal control problem to find the initial condition. If the model is ‘perfect,’ the optimal solution (analysis) error rises because of the presence of the input data errors (background and observation errors). Then, this error is quantified by the covariance matrix, which can be approximated by the inverse Hessian of an auxiliary control problem. If the model is not perfect, the optimal solution error includes an additional component because of the presence of the model error. In this paper, we study the influence of the model error on the optimal solution error covariance, considering strong and weak constraint data assimilation approaches. For the latter, an additional equation describing the model error dynamics is involved. Numerical experiments for the 1D Burgers equation illustrate the presented theory. Copyright © 2016 John Wiley & Sons, Ltd.
Distribution and diffusion of surface suspended matter off the East China Shore, 2010
Authors: Liu Xue , Li Guangxue , Ma Yanyan , Ding Dong , Dada Olusegun A. , Xu Jishang , Qiao Lulu , Li Jianchao , Liu Shidong 
Published: June 2016

AbstractIt is a question whether the suspended sediment from the China continent could enter the Kuroshio Current and Pacific Ocean. Surface suspended matter (SSM) and sea surface temperature (SST) in the East China Seas from December 2009 to November 2010 has been extracted from the satellite NOAA‐19 AVHRR/3. We have monitored the change of the SSM diffusion during summer half‐year and winter half‐year. High SSM areas occur in the inshore of the Liaodong Bay, Bohai Bay, Yellow River Delta, Shandong Peninsula, Jiangsu Coast and Yangtze Estuary, especially blooming in winter half‐year. Going by our findings, the sediment provenance influences the SSM distribution, and the current system and monsoon are important factors that drive the SSM diffusion all year round. In winter half‐year, the ocean colour images show that the current system is well developed. The SSMs are transported southeastward, and a part reaching the outer continental shelf is captured by the Kuroshio Current before it finally reaches the open Pacific Ocean through the Tokara Strait. Some SSMs are transported by the Yellow Sea and Tsushima Warm Current into the Yellow Sea and Japan Sea. In summer half‐year, most of the SSM could not reach the continental shelf break and only little SSM could be captured by the Kuroshio Current into the open ocean. Copyright © 2016 John Wiley & Sons, Ltd.
Curcumin inhibits aerobic glycolysis in hepatic stellate cells associated with activation of adenosine monophosphate‐activated protein kinase
Authors: Lian Naqi , Jin Huanhuan , Zhang Feng , Wu Li , Shao Jiangjuan , Lu Yin , Zheng Shizhong 
Published: June 2016

SummaryActivation of hepatic stellate cells (HSCs) is characterized by expression of extracellular matrix and loss of adipogenic phenotype during liver fibrogenesis. Emerging evidence suggests that HSCs adopt aerobic glycolysis during activation. The present work aimed at investigating whether the anti‐fibrogenic effects of curcumin was associated with interfering with glycolysis in HSCs. Primary rat HSCs were cultured in vitro. We demonstrated that inhibition of glycolysis by 2‐deoxyglucose or galloflavin reduced the expression of α‐smooth muscle actin (α‐SMA) and α1(I)procollagen at both mRNA and protein levels, and increased the intracellular lipid contents and upregulated the gene and protein expression of adipogenic transcription factors C/EBPα and PPAR‐γ in HSCs. Curcumin at 20 μM produced similar effects. Moreover, curcumin decreased the expression of hexokinase (HK), phosphofructokinase‐2 (PFK2), and glucose transporter 4 (glut4), three key glycolytic parameters, at both mRNA and protein levels. Curcumin also reduced lactate production concentration‐dependently in HSCs. Furthermore, curcumin increased the phosphorylation of adenosine monophosphate‐activated protein kinase (AMPK), but AMPK inhibitor BML‐275 significantly abolished the curcumin downregulation of HK, PFK2, and glut4. In addition, curcumin inhibition of α‐SMA and α1(I)procollagen was rescued by BML‐275, and curcumin upregulation of C/EBPα and PPAR‐γ was abrogated by BML‐275. These results collectively indicated that curcumin inhibited glycolysis in an AMPK activation‐dependent manner in HSCs. We revealed a novel mechanism for curcumin suppression of HSC activation implicated in antifibrotic therapy. © 2016 IUBMB Life, 68(7):589–596, 2016
Monthly rain amounts at Fabra Observatory (Barcelona, NE Spain): fractal structure, autoregressive processes and correlation with monthly Western Mediterranean Oscillation index
Authors: Lana X. , Burgueño A. , Martínez M. D. , Serra C. 
Published: June 2016

ABSTRACTMonthly rain amounts (MRA) recorded at Fabra Observatory (Barcelona, NE Spain) since 1917–2010, are analysed from two complementary points of view. First, mono‐ and multifractal characteristics of MRA are compared with those corresponding to the monthly Western Mediterranean Oscillation index (WeMOi), which affects the rainfall regime at the western Mediterranean region. Monofractality is analysed through Hurst and Hausdorff exponents, and a power law describing the dependence of MRA power spectra on frequency. The reconstruction theorem permits to quantify the complexity of the physical process by means of the correlation dimension and the Kolmogorov entropy. In agreement with this theorem, the predictive instability is also evaluated through the Lyapunov exponents. The multifractality is characterized by the critical Hölder exponent, as well as by the asymmetry and spectral width of the multifractal spectrum. Second, three predictive processes for MRA are tested. One of them is based on the assumption that MRA could be simulated by fractional Gaussian noise. The other two are, by one hand, an ARIMA(p,1,0) process for MRA; on the other hand, an adaptation of the ARIMA process for MRA taking as arguments preceding WeMOi. Finally, relationships between MRA and WeMOi confirm that outstanding MRA use to be coincident with negative WeMOi. An explanation is also proposed for the non‐negligible number of MRA corresponding to positive WeMOi.
Cytocompatible, Photoreversible, and Self‐Healing Hydrogels for Regulating Bone Marrow Stromal Cell Differentiation
Authors: Yu Lianlian , Xu Kaige , Ge Liangpeng , Wan Wenbing , Darabi Ali , Xing Malcolm , Zhong Wen 
Published: June 2016

Photo‐crosslinking and self‐healing have received considerable attention for the design of intelligent materials. A novel photostimulated, self‐healing, and cytocompatible hydrogel system is reported. A coumarin methacrylate crosslinker is synthesized to modify the polyacrylamide‐based hydrogels. With the [2+2] cyclo‐addition of coumarin moieties, the hydrogels exhibit excellent self‐healing capacity when they are exposed to light with wavelengths at 280 and 365 nm, respectively. To enhance cell compatibility, a poly (amidoamine) crosslinker is also synthesized. Variations in light exposure times and irradiation wavelengths are found to alter the self‐healing property of the hydrogels. The hydrogels are shown to induce a regular cellular pattern. The hydrogels are used to regulate bone marrow stromal cells differentiation. The relative mRNA expressions are recorded to monitor the osteogenic differentiation of the cells.
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Tuning the Surface of Nanoparticles: Impact of Poly(2‐ethyl‐2‐oxazoline) on Protein Adsorption in Serum and Cellular Uptake
Authors: Koshkina Olga , Westmeier Dana , Lang Thomas , Bantz Christoph , Hahlbrock Angelina , Würth Christian , Resch‐Genger Ute , Braun Ulrike , Thiermann Raphael , Weise Christoph , Eravci Murat , Mohr Benjamin , Schlaad Helmut , Stauber Roland H. , Docter Dominic , Bertin Annabelle , Maskos Michael 
Published: June 2016

Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2‐ethyl‐2‐oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non‐coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi‐angle dynamic light scattering, asymmetrical flow field‐flow fractionation, gel electrophoresis, and liquid chromatography‐mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non‐specific cellular uptake, particularly by macrophage‐like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
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Empathic Relationships in Professional Services and the Moderating Role of Relationship Age
Authors: Weißhaar Isabelle , Huber Frank 
Published: June 2016

ABSTRACTEmpathy has been identified as a key success factor for employees who interact with customers. Despite its overall relevance, only a few studies have acknowledged its multidimensional nature. Knowledge remains scarce about the relative impact of cognitive and affective empathy on relational outcomes. In addition, few pieces of research have explicitly acknowledged empathy as theorized within stage‐models focusing on the communication of empathy. The authors conceptualize empathy as a multidimensional construct perceived by the client. Empathy has to be communicated to be effective, while customers’ perceptions of empathy may also be more complex as they distinguish between cognitive and affective empathy. This article investigates the effects of perspective taking, emotional concern, and emotional contagion on trust and commitment. Drawing upon relationship stage concepts, the authors further argue that the influence is moderated by relationship age. Finally, this study investigates if employees benefit from being perceived as empathic partners in terms of “hard facts” (objective sales performance). Based on a data set from 215 business clients of a large consulting firm, this study adopts a structural equation modeling (SEM) approach by using multigroup analysis. To test the empathy–performance link, the authors aggregate customer responses nested in 84 employees and link perceived empathy with performance data. The results show that within B2B relationships, perspective taking exerts the strongest influence on trust, whereas emotional concern is the strongest driver for commitment. The results also confirm the moderating role of relationship age and that perspective taking leads to an increase in actual sales performance. This study underpins the relevance of empathy within services marketing, while providing a more detailed approach to account for empathy as a relation building tool. Practical and academic implications are also addressed.
Radiation‐Induced In Situ Synthesis of Gold Nanostructured Materials
Authors: Ali Zakaria Ismaiel , Eisa Weal H. , Saleh Hoda H. , Meligi Gamal A. , El‐Sokary Rehab 
Published: June 2016

SummaryGold nanoparticles were in situ synthesized via irradiation process, using PVA, (PVA:Cs), and (PVA:PAM) as host polymeric matrices. The effect of irradiation dose, polymer content, polymer combination AgNO3, chitosan, and poly acrylamide were studied. Gold nanoparticles (AuNPs) have spherical nanoparticles shape with different size ranging from 10−35 nm, which were characterized by ultraviolet‐visible (UV‐VIS) spectroscopy, X‐ray spectroscopy and transmission electron microscopy. Fourier transform infrared spectroscopy (FTIR) shows the spectral shift together with reduced intensity of the −OH stretching vibration upon incorporation of the Au nanoparticles indicating the coordination between Au nanoparticles and the OH groups originating from the PVA chains. The synthesized gold nanoparticles have been successfully applied as a catalyst in the degradation of methyl orange and methylene blue by NaBH4.