bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brief Report: Radiofrequency Ablation Therapy for Anal Intraepithelial Neoplasia: Results From a Single-Center Prospective Pilot Study in HIV+ Participants
Authors: Goldstone Robert N. , Hasan Shirin R. , Goldstone Stephen E. 
Published: August 2017

Background:
HIV-positive individuals have high incidence of anal high-grade squamous intraepithelial lesions (HSIL) at the squamocolumnar junction (SCJ), which can progress to cancer. Focal radiofrequency ablation (RFA) treats HSIL, but metachronous recurrence remains high and may be improved with circumferential treatment.

Setting:
The study was performed at a single center.

Methods:
This was a prospective trial of circumferential anal RFA using Barrx 60 RFA focal catheters in participants with ≥1 anal SCJ HSIL. The entire SCJ was ablated by RFA. Adverse events (AEs), symptoms, including pain, and quality of life were assessed. High-resolution anoscopy assessed recurrences at 3, 6, 9, and 12 months. Lesion site biopsies occurred at month 12. Recurrences were retreated with focal RFA.

Results:
Ten male participants (9 HIV+), with a median 2 HSILs (range 2–8) each, enrolled. Median T-cell count and viral load were 730 cells/mcL and 38 copies/mL. Median duration of RFA treatment was 6.5 (5–13) minutes. Lesion persistence occurred in 4 participants (3 at 3 months, 1 at 6 months). Recurrence at a new site occurred in 1 participant at 3 months. No lesion persisted after retreatment. All participants were HSIL free and completely healed by 12 months. Two device-related mild AEs occurred in 1 participant each (thrombosed external hemorrhoid and soft anal scar; both resolved). No serious AEs occurred.

Conclusion:
Circumferential anal SCJ RFA produced total HSIL eradication with no more than 2 treatments. Circumferential RFA seems to be well tolerated with minimal pain and no serious AEs in HIV+ participants.

Clinicaltrials.gov:
NCT02189161.
Transnasal Endoscopic Resection of a Pleomorphic Adenoma Originate From Nasal Floor
Authors: Cho Yesun , Kim Yul Gyun , Shin Eunhye , Kim Boo-Young 
Published: August 2017

Abstract
A 81-year-old female presented to our hospital frequent epistaxis. Nasal endoscopy showed a mass obstructing nasal cavity completely and occupying middle meatus. Magnetic resonance imaging was performed, an about 4.8 × 4 × 4.2 cm sized heterogeneous T2 high signal intensity and T1 enhancing mass mainly involving right nasal cavity with invasion of right hard palate with bony destruction. Therefore, the authors planned to do endoscopic mass excision, under general anesthesia for diagnosis and treatment. The authors removed the mass from lateral nasal wall, nasal roof, nasal septum, medial maxillary wall by piece-meal. Margins of mass were clear except the nasal floor. So, the authors did frozen biopsy to confirm the clear margin in nasal floor. Endoscopy enables better visualization of tumor margins, facilitating complete removal and avoiding excessive resection and following up using good visualization.
Does Septoplasty Improve Heart Functions of Patients With Septum Deviation in Terms of Echocardiography Findings?
Authors: Sahin Murat Sertan , Ozmen Omer Afsin , Yalcin Muhammed Ulvi 
Published: August 2017

Objective:The authors aimed to determine pulmonary artery pressure and right heart functions in patients with nasal septum deviation (NSD) with echocardiography (EchoCG) and compare the postoperative changes of EchoCG parameters with preoperative findings.Methods:Seventy-six patients who underwent septoplasty composed the study group. Average age of patients was 23.50 ranging between 18 and 48 years of age. There were 53 males (69.7%) and 23 females (30.3%). Mean pulmonary artery pressure, tricuspid annular-plane systolic excursion, right ventricular diameter, and e/a ratio were measured by EchoCG preoperatively and same parameters were reobtained 3 months after surgery.Results:Mean pulmonary artery pressure reduced from 23.88 ± 6.36 to 19.80 ±3.95 mm Hg and tricuspid annular-plane systolic excursion increased from 22.36 ±3.85 to 23.57±3.00 3 months after surgery. It represented statistically significant improvement at right heart functions postoperatively (P < 0.001). Although they were not statistically significant, there was also some improvement in right ventricular diameter and e/a ratio values postoperatively.Conclusion:Nasal septum deviation was associated with higher PAP values, which were improved after surgery. Although at a lower extent, a negative effect of NSD on right heart functions was also suspected. Thus, treatment of NSD without delay was proposed, not only to treat the nasal symptoms but possible future cardiovascular complications as well.
Levator Aponeurosis and Muller Muscle Plication Reinforced With Levator Sheath Advancement for Blepharoptosis Correction
Authors: Byun Jin Suk , Hwang Kun , Lee Sang Yun , Kim Hak Tae , Kim Kenneth 
Published: August 2017

Abstract
The authors innovated the levator aponeurosis and Muller muscle plication reinforced with levator sheath advancement (AMPSA) for blepharoptosis correction. The orbital septum was opened 1 mm above its fusion with the levator aponeurosis. The preaponeurotic fat was retracted and the thickened part of the levator sheath was identified. Two plication sutures were made: medial suture at the medial border of the pupil and lateral between the lateral border of the pupil and the lateral limbus. A needle with 6-0 nylon thread first bit the tarsal plate approximately 1 mm below its upper border, then bit the levator aponeurosis and the Muller muscle together at 3 to 6 mm above the upper border of the tarsal plate. The needle bit 1 to 3 mm of the thickened part of the levator sheath and the suture was tied. A total of 116 eyes were operated on using levator aponeurosis and Muller muscle plication (AMP), and 79 eyes using AMPSA. The mean follow-up period was 11.4 months. In the AMP group, the postoperative marginal reflex distance-1 (MRD-1) (3.8 ± 0.2 mm) was significantly greater than the preoperative MRD-1 (2.7 ± 0.3 mm) (P < 0.001). In the AMPSA group, the postoperative MRD-1 (3.5 ± 0.3 mm) was also significantly greater than the preoperative MRD-1 (1.7 ± 0.4 mm) (P < 0.001). The improvement in MRD-1 was greater in the AMPSA group (1.7 ± 0.4 mm) than in the AMP group (1.1 ± 0.3 mm) (P < 0.001). The difference in the MRD-1 outcome between AMPSA and AMP (0.6 mm) was obtained by advancing the thickened part of the levator sheath. AMPSA may be an effective procedure for correcting blepharoptosis.
Cervical Necrotizing Fasciitis of Odontogenic Origin and Hyperbaric Oxygen Therapy
Authors: Inan Caner Hakki , Yener Haydar Murat , Yilmaz Mehmet , Gözen Emine Deniz , Erdur Zülküf Burak , Oroğlu Bengüsu , Olcay Eren , Memmedova Nermin 
Published: August 2017

Abstract
Necrotizing fasciitis (NF) is an infection of the soft tissues pathology with high mortality that spreads through the fascial planes and rarely seen in head and neck region. The cause of infection is often odontogenic problems. Broad-spectrum antibiotics, surgical debridement, and hyperbaric oxygen therapy (HBO) are important for the treatment of disease. We report a case of cervical NF in a 33-year-old woman who was treated with rapid surgical debridement and simultaneous HBO.
Differential Diagnosis of Intramuscular Schwannoma of the Craniocervical Region
Authors: Jeong Yeon Jin , Ko Hyo-Sun , Shim Hyung-Sup 
Published: August 2017

Abstract
Schwannomas in the head and neck are a relatively common condition. However, intramuscular schwannomas in the craniocervical region are extremely rare. The preoperative consideration of schwannomas is very important to preserve adjacent circulation and nerve function during the surgery in this area. Moreover, the treatment and preoperative evaluation of this condition is not firmly established in the past literatures. The authors successfully managed the intramuscular schwannoma in the craniocervical region, and provide the differential diagnoses with appropriate treatments.
A Randomized Double-Blind Placebo-Controlled Phase III Trial of Selegiline Monotherapy for Early Parkinson Disease
Authors: Mizuno Yoshikuni , Hattori Nobutaka , Kondo Tomoyoshi , Nomoto Masahiro , Origasa Hideki , Takahashi Ryosuke , Yamamoto Mitsutoshi , Yanagisawa Nobuo 
Published: August 2017

Background
In Japan, selegiline has been approved for combination therapy with levodopa for Parkinson disease (PD). We conducted a trial of selegiline monotherapy for early PD.

Methods
In this 12-week controlled phase III trial, a total of 292 subjects were randomized to receive placebo (n = 146) (full analysis set 140) or selegiline (n = 146) (full analysis set 139). The primary outcome measure was the change in the Unified Parkinson Disease Rating Scale part I + II + III total score from baseline to the final visit. Other secondary measures and a safety profile were evaluated.

Results
Selegiline monotherapy reduced the primary outcome measure by −6.26 ± 7.86 compared with the placebo −3.14 ± 6.98 (mean ± SD, P = 0.0005 by analysis of covariance). There was no significant difference in the number of adverse events between the 2 groups (P > 0.05).

Conclusions
Selegiline monotherapy reduced the total Unified Parkinson Disease Rating Scale part I + II + III score and was well tolerated in Japanese patients with early PD.
Nucleosome remodelling, DNA repair and transcriptional regulation build negative feedback loops in cancer and cellular ageing
Authors: Watanabe Reiko , Kanno Shin-ichiro , Mohammadi Roushandeh Amaneh , Ui Ayako , Yasui Akira 
Published: August 2017

Nucleosome remodelling (NR) regulates transcription in an ATP-dependent manner, and influences gene expression required for development and cellular functions, including those involved in anti-cancer and anti-ageing processes. ATP-utilizing chromatin assembly and remodelling factor (ACF) and Brahma-associated factor (BAF) complexes, belonging to the ISWI and SWI/SNF families, respectively, are involved in various types of DNA repair. Suppression of several BAF factors makes U2OS cells significantly sensitive to X-rays, UV and especially to cisplatin, and these BAF factors contribute to the accumulation of repair proteins at various types of DNA damage and to DNA repair. Recent cancer genome sequencing and expression analysis has shown that BAF factors are frequently mutated or, more frequently, silenced in various types of cancer cells. Thus, those cancer cells are potentially X-ray- and especially cisplatin-sensitive, suggesting a way of optimizing current cancer therapy. Recent single–stem cell analysis suggests that mutations and epigenetic changes influence stem cell functionality leading to cellular ageing. Genetic and epigenetic changes in the BAF factors diminish DNA repair as well as transcriptional regulation activities, and DNA repair defects in turn negatively influence NR and transcriptional regulation. Thus, they build negative feedback loops, which accelerate both cellular senescence and transformation as common and rare cellular events, respectively, causing cellular ageing.
This article is part of the themed issue ‘Chromatin modifiers and remodellers in DNA repair and signalling’.
Rational cell culture optimization enhances experimental reproducibility in cancer cells
Authors: Muelas Marina Wright , Ortega Fernando , Breitling Rainer , Bendtsen Claus , Westerhoff Hans V. 
Published: August 2017

AbstractOptimization of experimental conditions is critical in ensuring robust experimental reproducibility. Through detailed metabolomic analysis we found that cell culture conditions significantly impacted on glutaminase (GLS1) sensitivity resulting in variable sensitivity and irreproducibility in data.Baseline metabolite profiling highlighted that untreated cells underwent significant changes in metabolic status. Both the extracellular levels of glutamine and lactate and the intracellular levels of multiple metabolites changed drastically during the assay. We show that these changes compromise the robustness of the assay and make it difficult to reproduce.We then devised ‘metabolically rationalized standard’ assay conditions, in which glutaminase-1 inhibition reduced glutamine metabolism differently in both cell lines assayed, and decreased the proliferation of one of them. The adoption of optimized conditions such as the ones described here should lead to an improvement in reproducibility and help eliminate false negatives as well as false positives in these assays.
On the relationship between instantaneous phase synchrony and correlation-based sliding windows for time-resolved fMRI connectivity analysis
Authors: Pedersen Mangor , Omidvarnia Amir , Zalesky Andrew , Jackson Graeme D. 
Published: August 2017

AbstractCorrelation-based sliding window analysis (CSWA) is the most commonly used method to estimate time-resolved functional MRI (fMRI) connectivity. However, instantaneous phase synchrony analysis (IPSA) is gaining popularity mainly because it offers single time-point resolution of time-resolved fMRI connectivity. We aim to provide a systematic comparison between these two approaches, on both temporal and topological levels.For this purpose, we used resting-state fMRI data from two separate cohorts with different temporal resolutions (45 healthy subjects from Human Connectome Project fMRI data with repetition time of 0.72 s and 25 healthy subjects from a separate validation fMRI dataset with a repetition time of 3 s). For time-resolved functional connectivity analysis, we calculated tapered CSWA over a wide range of different window lengths that were temporally and topologically compared to IPSA.We found a strong association in connectivity dynamics between IPSA and CSWA when considering the absolute values of CSWA. This association peaked at a CSWA window length of ∼20 seconds, irrespective of the sampling rate of the underlying fMRI data. Narrow-band filtering of fMRI data (0.03-0.07 Hz) yielded a stronger relationship between IPSA and CSWA than wider-band (0.01-0.1 Hz). On a topological level, time-averaged IPSA and CSWA nodes were non-linearly correlated, mainly because nodes with strong negative correlations (CSWA) displayed high phase synchrony (IPSA).Our results suggest that IPSA and CSWA provide comparable characterizations of time-resolved fMRI connectivity for appropriately chosen window lengths. Although IPSA requires narrow-band fMRI filtering, we recommend the use of IPSA given that it does not mandate a (semi-)arbitrary choice of window length and window overlap. A MATLAB code for calculating IPSA is provided.
Common features in plastic changes rather than constructed structures in recurrent neural network prefrontal cortex models
Authors: Kuroki Satoshi , Isomura Takuya 
Published: August 2017

AbstractWe have flexible control over our cognition depending on the context or surrounding environments. The prefrontal cortex (PFC) controls this cognitive flexibility; however, the detailed underlying mechanisms remain unclear. Recent developments in machine learning techniques have allowed simple recurrent neural network PFC models to perform human- or animal-like behavioral tasks. These systems allow us to acquire parameters, which we could not in biological experiments, for performing the tasks. We compared four models, in which a flexible cognition task, called context-dependent integration task, was performed; subsequently, we searched for common features. In all the models, we observed that high plastic synapses were concentrated in the small neuronal population and the more concentrated neuronal units contributed further to the performance. However, there were no common properties in the constructed structures. These results suggest that plastic changes can be more general and important to accomplish cognitive tasks than features of the constructed structures.
Development of High-Throughput Screening Assays for Inhibitors of ETS Transcription Factors
Authors: Currie Simon L. , Warner Stephen L. , Vankayalapati Hariprasad , Liu Xiao-Hui , Sharma Sunil , Bearss David J. , Graves Barbara J. 
Published: August 2017

AbstractETS transcription factors from the ERG and ETV1/4/5 subfamilies are overexpressed in the majority of prostate cancer patients and contribute to disease progression. Here, we develop twoin vitroassays for the interaction of ETS transcription factors with DNA that are amenable for high throughput screening. Using ETS1 as a model, these assays were applied to screen 110 compounds derived from a high-throughput virtual screen. We find that the use of lower affinity DNA-binding sequences, similar to those which ERG and ETV1 bind to in prostate cells, allowed for higher inhibition from many of these test compounds. Further pilot experiments demonstrated that thein vitroassays are robust for ERG, ETV1, and ETV5, three of the ETS transcription factors that are overexpressed in prostate cancer.
Human macrophages survive and adopt activated genotypes in living zebrafish
Authors: Paul Colin D , Devine Alexus , Bishop Kevin , Xu Qing , Wulftange William J , Burr Hannah , Daly Kathryn M , Lewis Chaunte , Green Daniel S , Staunton Jack R , Choksi Swati , Liu Zheng-Gang , Sood Raman , Tanner Kandice 
Published: August 2017

AbstractThe inflammatory response, modulated both by tissue resident macrophages and recruited monocytes from peripheral blood, plays a critical role in human diseases such as cancer and neurodegenerative disorders. Here, we sought a model to interrogate human immune behaviorin vivo. We determined that primary human monocytes and macrophages survive in zebrafish for up to two weeks. Flow cytometry revealed that human monocytes cultured at the physiological temperature of the zebrafish survive and differentiate comparable to cohorts cultured at human physiological temperature. Moreover, key genes that encode for proteins that play a role in tissue remodeling were also expressed. Human cells migrated within multiple tissues at speeds comparable to zebrafish macrophages. Analysis of gene expression ofin vivoeducated human macrophages confirmed expression of activated macrophage phenotypes. Here, human cells adopted phenotypes relevant to cancer progression, suggesting that we can define the real time immune modulation of human tumor cells during the establishment of a metastatic lesion in zebrafish.
Tracing the color line in the American music market and its effect on contemporary music marketing
Authors: Barretta Paul G. 
Published: August 2017

PurposeThe purpose of this paper is to provide an in-depth consideration of the color line in the US music market, much deeper treatment than that of a superficial social construct.Design/methodology/approachContent analysis was performed using archives from the Performing Arts Division of the New York Public Library.FindingsA complex intersection of social and capitalist influences is fueled by culture and economics, filtered through the contributions of artists and media. Six major categories: social, media, artist, culture, industry, and economics contribute to its development and propagation. It continues to affect contemporary music markets.Research limitations/implicationsInterpretation of archival data is subject to availability of material and subjectivity of the researcher. Steps were taken to minimize bias. The research implies an opportunity for the US music market to celebrate diversity and social justice.Practical implicationsFocusing on the symbolic use of music, marketers have the opportunity to empower consumers to embrace diversity, reversing the trajectory of the color line.Social implicationsEmbracing cultural heritage and celebrating diversity can promote economic gain without detriment to cultural interests.Originality/valueThe present research provides a much deeper consideration of the color line in the American Music Market than previous literature does. The consideration includes a combination of forces, from profit focused to cultural.
Socioeconomic development in the context of Uruguay: a knowledge-based approach
Authors: Duarte Alonso Abel 
Published: August 2017

PurposeThe purpose of this exploratory study is to examine the relevance and impact of knowledge in the context of Uruguay’s present and future socioeconomic development through the lens of the knowledge-based theory of the firm (KBTF).Design/methodology/approachThe perspectives of 47 key informants, predominantly representatives of public and private Uruguayan institutions, including chambers of commerce and producer associations, were gathered through unstructured, face-to-face interviews.FindingsAligned with the KBTF, the significance of tacit knowledge, complemented with explicit knowledge, was revealed, particularly in the more traditional industries. Indeed, industry-based (tacit) knowledge evolving for generations has been strengthened by innovative practices, enhancing the appeal and image of key commodities and the nation’s exports. Additional elements highlighted in the KBTF, such as problem-solving, knowledge integration and application and knowledge specialisation, were identified.Originality/valueEssentially, the study highlights the different associations between the KBTF, the various forms of acquiring knowledge (tacit, explicit), innovation and resulting impacts on food quality and increased product recognition for a developing economy. Moreover, the findings, which illustrate that crucial improvements can be achieved through knowledge-based approaches, could also be considered in the context of other emerging economies that are aiming to attain further socioeconomic development through maximising the benefits of knowledge. In addition, the study addresses a theme that has been sporadically presented in the academic literature, especially when studying developing economies and their industries.
Identifying key success factors in supply chain management for increasing the competitive advantages of Vietnamese coffee
Authors: Nguyen Thich Van , Nguyen Nam Cao , Bosch Ockie J.H. 
Published: August 2017

Purpose
The purpose of this paper is to identify the leverage points in the Vietnamese coffee supply chain (SC) that would be used for increasing competitive advantages of the product.

Design/methodology/approach
A sequential approach by combining two established modelling techniques (causal loop diagram and Bayesian belief networks) was applied to identify the leverage points in the Vietnamese coffee SC for increasing the competitive advantages of the product. Data for the study were collected from a series of workshops and in-depth interviews with numerous relevant stakeholders of the coffee industry in the central highland of Vietnam.

Findings
The systems’ archetypes were developed and a sensitivity analysis was conducted to identify potential factors that can increase the competitive advantages of coffee production. The results indicate that higher investment in all elements of the coffee SC will lead to an enhanced competitive advantage.

Originality/value
Supply chain management (SCM) has become a potentially valuable method for improving competitive advantages, as competition is no longer only between organizations but also among SCs. Therefore, this research focus on enhancing the competitive advantages of production via SCM.