bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chill coma in the locust, Locusta migratoria, is initiated by spreading depolarization in the central nervous system
Authors: Robertson R. Meldrum , Spong Kristin E. , Srithiphaphirom Phinyaphat 
Published: August 2017

AbstractThe ability of chill-sensitive insects to function at low temperatures limits their geographic ranges. They have species-specific temperatures below which movements become uncoordinated prior to entering a reversible state of neuromuscular paralysis. In spite of decades of research, which in recent years has focused on muscle function, the role of neural mechanisms in determining chill coma is unknown. Spreading depolarization (SD) is a phenomenon that causes a shutdown of neural function in the integrating centres of the central nervous system. We investigated the role of SD in the process of entering chill coma in the locust, Locusta migratoria. We used thermolimit respirometry and electromyography in whole animals and extracellular and intracellular recording techniques in semi-intact preparations to characterize neural events during chilling. We show that chill-induced SD in the central nervous system is the mechanism underlying the critical thermal minimum for coordinated movement in locusts. This finding will be important for understanding how insects adapt and acclimate to changing environmental temperatures.
Mind wandering simultaneously prolongs reactions and promotes creative incubation
Authors: Leszczynski Marcin , Chaieb Leila , Reber Thomas P. , Derner Marlene , Axmacher Nikolai , Fell Juergen 
Published: August 2017

AbstractMind wandering (MW) refers to the disengagement of attention from the external environment and the generation of thoughts unrelated to the task at hand. It is a ubiquitous cognitive process resulting in lapses of attention. MW imposes a negative impact on attention-based task performance, but also has been associated with enhanced creativity and future planning. In three experiments we show that MW relates simultaneously to both behavioral costs but also benefits. Behavioral costs were measured by prolonged reaction times (RT) in sustained attention to response tasks (SART), whereas the benefits were observed as improved performance in the creative problem solving and daily routine planning tasks performed after the SART. Additionally, we found an increased dispersion of RTs during MW suggesting that attention during these times underwent dynamical changes compared to states when participants were fully focused on the task. Our results support a model in which MW deteriorates performance in the task at hand and is related to dynamical changes in attention. At the same time it is also able to improve human capacity for complex operations.
miR-96 attenuates status epilepticus-induced brain injury by directly targeting Atg7 and Atg16L1
Authors: Gan Jing , Cai Qianyun , Qu Yi , Zhao Fengyan , Wan Chaomin , Luo Rong , Mu Dezhi 
Published: August 2017

AbstractStatus epilepticus (SE) can cause brain damage and lead to neural dysfunction. Developing novel targets for SE therapy and diagnosis is important and necessary. Previously, we found several differentially expressed microRNAs (miRNAs) in the developing hippocampus following SE, including the autophagy-related miR-96. In the present study, we employed immunofluorescence staining and Western blot analysis to assess the expression of autophagy-related 7 (Atg7) and Atg16L1 and the status of autophagosome formation in the hippocampus of immature rats with SE. Additional in vivo intervention was also performed to investigate the potential therapeutic function of miR-96 in developing rats with SE. We found that Atg7 and Atg16L1 were up-regulated in the neurons after SE, together with an increase in autophagosome formation. Meanwhile, overexpression of miR-96 significantly prevented brain damage in SE rats by inhibiting Atg7 and Atg16L1 expression and autophagosome formation in the hippocampus. Furthermore, Rapamycin negated miR-96 mediated brain injury attenuation through inducing autophagosome formation. Our study indicates that miR-96 might be a potential target for therapy of pediatric SE.
Field-free manipulation of magnetization alignments in a Fe/GaAs/GaMnAs multilayer by spin-orbit-induced magnetic fields
Authors: Lee Sangyeop , Yoo Taehee , Bac Seul-Ki , Choi Seonghoon , Lee Hakjoon , Lee Sanghoon , Liu Xinyu , Dobrowolska Margaret , Furdyna Jacek K. 
Published: August 2017

AbstractWe investigate the process of selectively manipulating the magnetization alignment in magnetic layers in the Fe/GaAs/GaMnAs structure by current-induced spin-orbit (SO) magnetic field. The presence of such fields manifests itself through the hysteretic behavior of planar Hall resistance observed for two opposite currents as the magnetization in the structure switches directions. In the case of the Fe/GaAs/GaMnAs multilayer, hystereses are clearly observed when the magnetization switches direction in the GaMnAs layer, but are negligible when magnetization transitions occur in Fe. This difference in the effect of the SO-field in the two magnetic layers provides an opportunity to control the magnetization in one layer (in the presence case in GaMnAs) by a current, while the magnetization in the other layer (i.e., Fe) remains fixed. Owing to our ability to selectively control the magnetization in the GaMnAs layer, we are able to manipulate the relative spin configurations in our structure between collinear and non-collinear alignments simply by switching the current direction even in the absence of an external magnetic field.
One-pot preparation of nanoporous Ag-Cu@Ag core-shell alloy with enhanced oxidative stability and robust antibacterial activity
Authors: Liu Xue , Du Jing , Shao Yang , Zhao Shao-Fan , Yao Ke-Fu 
Published: August 2017

AbstractMetallic core–shell nanostructures have inspired prominent research interests due to their better performances in catalytic, optical, electric, and magnetic applications as well as the less cost of noble metal than monometallic nanostructures, but limited by the complicated and expensive synthesis approaches. Development of one-pot and inexpensive method for metallic core–shell nanostructures’ synthesis is therefore of great significance. A novel Cu network supported nanoporous Ag-Cu alloy with an Ag shell and an Ag-Cu core was successfully synthesized by one-pot chemical dealloying of Zr-Cu-Ag-Al-O amorphous/crystalline composite, which provides a new way to prepare metallic core–shell nanostructures by a simple method. The prepared nanoporous Ag-Cu@Ag core-shell alloy demonstrates excellent air-stability at room temperature and enhanced oxidative stability even compared with other reported Cu@Ag core-shell micro-particles. In addition, the nanoporous Ag-Cu@Ag core-shell alloy also possesses robust antibacterial activity against E. Coli DH5α. The simple and low-cost synthesis method as well as the excellent oxidative stability promises the nanoporous Ag-Cu@Ag core-shell alloy potentially wide applications.
Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι
Authors: Miropolskaya Nataliya , Petushkov Ivan , Kulbachinskiy Andrey , Makarova Alena V. 
Published: August 2017

AbstractBesides X-family DNA polymerases (first of all, Pol β) several other human DNA polymerases from Y- and A- families were shown to possess the dRP-lyase activity and could serve as backup polymerases in base excision repair (Pol ι, Rev1, Pol γ and Pol θ). However the exact position of the active sites and the amino acid residues involved in the dRP-lyase activity in Y- and A- family DNA polymerases are not known. Here we carried out functional analysis of fifteen amino acid residues possibly involved in the dRP-lyase activity of human Pol ι. We show that substitutions of residues Q59, K60 and K207 impair the dRP-lyase activity of Pol ι while residues in the HhH motif of the thumb domain are dispensable for this activity. While both K60G and K207A substitutions decrease Schiff-base intermediate formation during dRP group cleavage, the latter substitution also strongly affects the DNA polymerase activity of Pol ι, suggesting that it may impair DNA binding. These data are consistent with an important role of the N-terminal region in the dRP-lyase activity of Pol ι, with possible involvement of residues from the finger domain in the dRP group cleavage.
Interleukin-6-dependent growth in a newly established plasmablastic lymphoma cell line and its therapeutic targets
Authors: Mine Sohtaro , Hishima Tsunekazu , Suganuma Akihiko , Fukumoto Hitomi , Sato Yuko , Kataoka Michiyo , Sekizuka Tsuyoshi , Kuroda Makoto , Suzuki Tadaki , Hasegawa Hideki , Fukayama Masashi , Katano Harutaka 
Published: August 2017

AbstractPlasmablastic lymphoma (PBL) is a rare, highly aggressive subtype of non-Hodgkin lymphoma with plasma-cell differentiation occurring typically in immune-suppressed patients such as those with AIDS. This study reports the establishment and characterization of a new cell line, PBL-1, derived from a patient with AIDS-associated PBL. Morphological assessment of PBL-1 indicated plasma-cell differentiation with a CD20(−) CD38(+) CD138(+) immunophenotype and IgH/c-myc translocation. The cell line harbours Epstein-Barr virus, but a 52.7-kbp length defect was identified in its genome, resulting in no expression of viral microRNAs encoded in the BamHI-A Rightward Transcript region. Importantly, supplementation of culture medium with >5 ng/mL of interleukin-6 (IL-6) was required for PBL-1 growth. Starvation of IL-6 or addition of tocilizumab, an inhibitory antibody for the IL-6 receptor, induced apoptosis of PBL-1. Transduction of IL-6 into PBL-1 by lentivirus vector induced autologous growth without IL-6 supplementation of culture medium. These data indicate the IL-6 dependency of PBL-1 for proliferation and survival. mTOR inhibitors induced cell death effectively, suggesting mTOR in the IL-6 signalling pathway is a potential therapeutic target for PBL. This established PBL cell line will be a useful tool to further understand the pathophysiology of PBL and aid the future development of PBL treatment.
Differential Cortical Gray Matter Deficits in Adolescent- and Adult-Onset First-Episode Treatment-Naïve Patients with Schizophrenia
Authors: Zhang Chengcheng , Wang Qiang , Ni Peiyan , Deng Wei , Li Yinfei , Zhao Liansheng , Ma Xiaohong , Wang Yingcheng , Yu Hua , Li Xiaojing , Zhang Pingping , Meng Yajing , Liang Sugai , Li Mingli , Li Tao 
Published: August 2017

AbstractThe current study aimed to explore age-variant trait differences of cortical gray matter volume (GMV) in a unique sample of first-episode and treatment-naïve patients with schizophrenia. A total of 158 subjects, including 26 adolescent-onset patients and 49 adult-onset patients as well as 83 age- and gender-matched controls were scanned using a 3T MRI scanner. Voxel-based morphometry (VBM) following Diffeomorphic Anatomical Registration Through Exponentiated Lie algebra (DARTEL) was used to explore group differences between patients and controls in regional GMV. We found that patients with schizophrenia had decreased GMV in the left parietal postcentral region that extended to the left frontal regions, the right middle temporal gyrus, the occipital lobe and the right cerebellum posterior pyramis. Further analysis showed a distinct pattern of gray matter alterations in adolescent-onset patients compared with both healthy controls and adult-onset patients. Relative to healthy controls, adolescent-onset patients showed GMV alterations in the left parietal postcentral gyrus, parahippocampal gyrus and right cerebellum posterior pyramis, while GMV deficits in adult-onset patients were focused on the cingulo-fronto-temporal module and right occipital regions. Our study identified differential cortical gray matter deficits between adolescent- and adulthood-onset patients with schizophrenia, which suggests that the cortical abnormalities in schizophrenia are likely adjusted by the developmental community structure of the human brain.
Topological N-glycosylation and site-specific N-glycan sulfation of influenza proteins in the highly expressed H1N1 candidate vaccines
Authors: She Yi-Min , Farnsworth Aaron , Li Xuguang , Cyr Terry D. 
Published: August 2017

AbstractThe outbreak of a pandemic influenza H1N1 in 2009 required the rapid generation of high-yielding vaccines against the A/California/7/2009 virus, which were achieved by either addition or deletion of a glycosylation site in the influenza proteins hemagglutinin and neuraminidase. In this report, we have systematically evaluated the glycan composition, structural distribution and topology of glycosylation for two high-yield candidate reassortant vaccines (NIBRG-121xp and NYMC-X181A) by combining various enzymatic digestions with high performance liquid chromatography and multiple-stage mass spectrometry. Proteomic data analyses of the full-length protein sequences determined 9 N-glycosylation sites of hemagglutinin, and defined 6 N-glycosylation sites and the glycan structures of low abundance neuraminidase, which were occupied by high-mannose, hybrid and complex-type N-glycans. A total of ~300 glycopeptides were analyzed and manually validated by tandem mass spectrometry. The specific N-glycan structure and topological location of these N-glycans are highly correlated to the spatial protein structure and the residential ligand binding. Interestingly, sulfation, fucosylation and bisecting N-acetylglucosamine of N-glycans were also reliably identified at the specific glycosylation sites of the two influenza proteins that may serve a crucial role in regulating the protein structure and increasing the protein abundance of the influenza virus reassortants.
Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure
Authors: Anjum Fraz , Agabalyan Natacha A. , Sparks Holly D. , Rosin Nicole L. , Kallos Michael S. , Biernaskie Jeff 
Published: August 2017

AbstractCell-based therapies have recently been the focus of much research to enhance skin wound healing. An important challenge will be to develop vehicles for cell delivery that promote survival and uniform distribution of cells across the wound bed. These systems should be stiff enough to facilitate handling, whilst soft enough to limit damage to newly synthesized wound tissue and minimize patient discomfort. Herein, we developed several novel modifiable nanofibre scaffolds comprised of Poly (ε-caprolactone) (PCL) and gelatin (GE). We asked whether they could be used as a functional receptacle for adult human Skin-derived Precursor Cells (hSKPs) and how naked scaffolds impact endogenous skin wound healing. PCL and GE were electrospun in a single facile solvent to create composite scaffolds and displayed unique morphological and mechanical properties. After seeding with adult hSKPs, deposition of extracellular matrix proteins and sulphated glycosaminoglycans was found to be enhanced in composite grafts. Moreover, composite scaffolds exhibited significantly higher cell proliferation, greater cell spreading and integration within the nanofiber mats. Transplantation of acellular scaffolds into wounds revealed scaffolds exhibited improvement in dermal-epidermal thickness, axonal density and collagen deposition. These results demonstrate that PCL-based nanofiber scaffolds show promise as a cell delivery system for wound healing.
The novel link between planar möbius aromatic and third order nonlinear optical properties of metal–bridged polycyclic complexes
Authors: Wang Li , Ye Jinting , Wang Hongqiang , Xie Haiming , Qiu Yongqing 
Published: August 2017

AbstractMetal–bridged polcyclic aromatic complexes, exhibiting unusual optical effects such as near-infrared photoluminescence with particularly large Stokes shifts, long lifetimes and aggregation enhancement, have been established as unique “carbonloong chemistry”. Herein, the electronic structures, aromaticities, absorption spectra and third order nonlinear optical (NLO) responses of metal–bridged polcyclic aromatic complexes (M = Fe, Re, Os and Ir) are investigated using the density functional theory computations. It is found that the bridge–head metal can stabilize and influence rings, thus creating π–, σ– and metalla–aromaticity in an extended, π–conjugated framework. Interestingly, metal radius greatly influence the bond, aromaticity, liner and third order NLO properties, which reveals useful information to develop new applications of metal regulatory mechanism in NLO materials field. Significantly, the novel relationship between the aromaticity and third order NLO response has firstly been proposed, that the metal-bridged polycyclic complex with larger aromaticity will exhibit larger third order nonlinear optical response. It is our expectation that the novel link between aromaticity and NLO response could provide valuable information for scientists to develop the potential NLO materials on the basis of metal–bridged polycyclic complexes.
RNA-seq analysis of apical meristem reveals integrative regulatory network of ROS and chilling potentially related to flowering in Litchi chinensis
Authors: Lu Xingyu , Li Jingjing , Chen Houbin , Hu Jiaqi , Liu Pengxu , Zhou Biyan 
Published: August 2017

AbstractLitchi is an important woody fruit tree. Floral initiation in litchi is triggered by low temperatures. However, defective flowering is a major challenge for litchi production in times of climate change and global warming. Previous studies have shown that the reactive oxygen species (ROS) generated by methyl viologen dichloride hydrate (MV) promotes flowering. In this study, potted trees were transferred to growth chambers for low-temperature (LT), medium-temperature (MT), and high-temperature (HT) treatments. Trees at MT were subjected to ROS treatment to promote flowering, and those at LT were induced to flower. RNA-sequencing was applied to obtain a global transcriptome of the apical meristem and reveal potential gene networks controlling the transformation from vegetative meristems (VM) into inflorescence meristems (IM). We assembled 73,117 unigenes with a mean size of 790 bp and 11741 unigenes were identified as both chilling and ROS responsive genes (CRRGs), of which 48 were identified as flowering-related CRRGs, 59 were plant hormone signal transduction CRRGs, and 146 were plant hormone biosynthesis-related CRRGs. Genes co-expression network analysis indicated inner relationships, suggesting that ROS and chilling promotes the VM to IM transition through a regulatory gene network of transcription factors, hormones, and flowering regulators.
Deswelling and deformation of microgels in concentrated packings
Authors: Bouhid de Aguiar I. , van de Laar T. , Meireles M. , Bouchoux A. , Sprakel J. , Schroën K. 
Published: August 2017

AbstractIncreasing the particle density of a suspension of microgel colloids above the point of random-close packing, must involve deformations of the particle to accommodate the increase in volume fraction. By contrast to the isotropic osmotic deswelling of soft particles, the particle-particle contacts give rise to a non-homogeneous pressure, raising the question if these deformations occur through homogeneous deswelling or by the formation of facets. Here we aim to answer this question through a combination of imaging of individual microgels in dense packings and a simple model to describe the balance between shape versus volume changes. We find a transition from shape changes at low pressures to volume changes at high pressures, which can be explained qualitatively with our model. Whereas contact mechanics govern at low pressures giving rise to facets, osmotic effects govern at higher pressures, which leads to a more homogeneous deswelling. Our results show that both types of deformation play a large role in highly concentrated microgel suspensions and thus must be taken into account to arrive at an accurate description of the structure, dynamics and mechanics of concentrated suspensions of soft spheres.
Short Chain Fatty Acids Enhance Aryl Hydrocarbon (Ah) Responsiveness in Mouse Colonocytes and Caco-2 Human Colon Cancer Cells
Authors: Jin Un-Ho , Cheng Yating , Park Hyejin , Davidson Laurie A. , Callaway Evelyn S. , Chapkin Robert S. , Jayaraman Arul , Asante Andrew , Allred Clinton , Weaver Evelyn A. , Safe Stephen 
Published: August 2017

AbstractAryl hydrocarbon receptor (AhR) ligands are important for gastrointestinal health and play a role in gut inflammation and the induction of T regulatory cells, and the short chain fatty acids (SCFAs) butyrate, propionate and acetate also induce similar protective responses. Initial studies with butyrate demonstrated that this compound significantly increased expression of Ah-responsive genes such as Cyp1a1/CYP1A1 in YAMC mouse colonocytes and Caco-2 human colon cancer cell lines. Butyrate synergistically enhanced AhR ligand-induced Cyp1a1/CYP1A1 in these cells with comparable enhancement being observed for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and also microbiota-derived AhR ligands tryptamine, indole and 1,4-dihydroxy-2-naphthoic acid (DHNA). The effects of butyrate on enhancing induction of Cyp1b1/CYP1B1, AhR repressor (Ahrr/AhRR) and TCDD-inducible poly(ADP-ribose)polymerase (Tiparp/TiPARP) by AhR ligands were gene- and cell context-dependent with the Caco-2 cells being the most responsive cell line. Like butyrate and propionate, the prototypical hydroxyamic acid-derived histone deacetylase (HDAC) inhibitors Panobinostat and Vorinostat also enhanced AhR ligand-mediated induction and this was accompanied by enhanced histone acetylation. Acetate also enhanced basal and ligand-inducible Ah responsiveness and histone acetylation, demonstrating that acetate was an HDAC inhibitor. These results demonstrate SCFA-AhR ligand interactions in YAMC and Caco-2 cells where SCFAs synergistically enhance basal and ligand-induced expression of AhR-responsive genes.
Microcystis aeruginosa strengthens the advantage of Daphnia similoides in competition with Moina micrura
Authors: Tang Hengxing , Hou Xinying , Xue Xiaofeng , Chen Rui , Zhu Xuexia , Huang Yuan , Chen Yafen 
Published: August 2017

AbstractMicrocystis blooms are generally associated with zooplankton shifts by disturbing interspecific relationships. The influence of Microcystis on competitive dominance by different sized zooplanktons showed species-specific dependence. We evaluated the competitive responses of small Moina micrura and large Daphnia similoides to the presence of Microcystis using mixed diets comprising 0%, 20%, and 35% of toxic M. aeruginosa, and the rest of green alga Chlorella pyrenoidosa. No competitive exclusion occurred for the two species under the tested diet combinations. In the absence of M. aeruginosa, the biomasses of the two cladocerans were decreased by the competition between them. However, the Daphnia was less inhibited with the higher biomass, suggesting the competitive dominance of Daphnia. M. aeruginosa treatment suppressed the population growths of the two cladocerans, with the reduced carrying capacities. Nonetheless, the population inhibition of Daphnia by competition was alleviated by the increased Microcystis proportion in diet. As a result, the competitive advantage of Daphnia became more pronounced, as indicated by the higher Daphnia: Moina biomass ratio with increased Microcystis proportions. These results suggested that M. aeruginosa strengthens the advantage of D. similoides in competition with M. micrura, which contributes to the diversified zooplankton shifts observed in fields during cyanobacteria blooms.