bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D plasmonic crystal metamaterials for ultra-sensitive biosensing
Authors: Aristov Andrey I. , Manousidaki Maria , Danilov Artem , Terzaki Konstantina , Fotakis Costas , Farsari Maria , Kabashin Andrei V. 
Published: May 2016

AbstractWe explore the excitation of plasmons in 3D plasmon crystal metamaterials and report the observation of a delocalized plasmon mode, which provides extremely high spectral sensitivity (>2600 nm per refractive index unit (RIU) change), outperforming all plasmonic counterparts excited in 2D nanoscale geometries, as well as a prominent phase-sensitive response (>3*104 deg. of phase per RIU). Combined with a large surface for bioimmobilization provided by the 3D matrix, the proposed sensor architecture promises a new important landmark in the advancement of plasmonic biosensing technology.
Expression of human Cfdp1 gene in Drosophila reveals new insights into the function of the evolutionarily conserved BCNT protein family
Authors: Messina Giovanni , Atterrato Maria Teresa , Fanti Laura , Giordano Ennio , Dimitri Patrizio 
Published: May 2016

AbstractThe Bucentaur (BCNT) protein family is widely distributed in eukaryotes and is characterized by a highly conserved C-terminal domain. This family was identified two decades ago in ruminants, but its role(s) remained largely unknown. Investigating cellular functions and mechanism of action of BCNT proteins is challenging, because they have been implicated in human craniofacial development. Recently, we found that YETI, the D. melanogaster BCNT, is a chromatin factor that participates to H2A.V deposition. Here we report the effects of in vivo expression of CFDP1, the human BCNT protein, in Drosophila melanogaster. We show that CFDP1, similarly to YETI, binds to chromatin and its expression results in a wide range of abnormalities highly reminiscent of those observed in Yeti null mutants. This indicates that CFDP1 expressed in flies behaves in a dominant negative fashion disrupting the YETI function. Moreover, GST pull-down provides evidence indicating that 1) both YETI and CFDP1 undergo homodimerization and 2) YETI and CFDP1 physically interact each other by forming inactive heterodimers that would trigger the observed dominant-negative effect. Overall, our findings highlight unanticipated evidences suggesting that homodimerization mediated by the BCNT domain is integral to the chromatin functions of BCNT proteins.
PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales
Authors: Shiraishi Miyako , Ishino Sonoko , Yoshida Kotaro , Yamagami Takeshi , Cann Isaac , Ishino Yoshizumi 
Published: May 2016

AbstractTo maintain genome integrity for transfer to their offspring and to maintain order in cellular processes, all living organisms have DNA repair systems. Besides the well-conserved DNA repair machineries, organisms thriving in extreme environments are expected to have developed efficient repair systems. We recently discovered a novel endonuclease, which cleaves the 5′ side of deoxyinosine, from the hyperthermophilic archaeon, Pyrococcus furiosus. The novel endonuclease, designated as Endonulcease Q (EndoQ), recognizes uracil, abasic site and xanthine, as well as hypoxanthine and cuts the phosphodiester bond at their 5′ sides. To understand the functional process involving EndoQ, we searched for interacting partners of EndoQ and identified Proliferating Cell Nuclear Angigen (PCNA). The EndoQ activity was clearly enhanced by addition of PCNA in vitro. The physical interaction between the two proteins through a PIP-motif of EndoQ and the toroidal structure of PCNA are critical for the stimulation of the endonuclease activity. These findings provide us a clue to elucidate a unique DNA repair system in Archaea.
Evolved resistance to colistin and its loss due to genetic reversion in Pseudomonas aeruginosa
Authors: Lee Ji-Young , Park Young Kyoung , Chung Eun Seon , Na In Young , Ko Kwan Soo 
Published: May 2016

AbstractThe increased reliance on colistin for treating multidrug-resistant Gram-negative bacterial infections has resulted in the emergence of colistin-resistantPseudomonas aeruginosa. We attempted to identify genetic contributors to colistin resistancein vitroevolved isogenic colistin-resistant and -susceptible strains of twoP. aeruginosalineages (P5 and P155). Their evolutionary paths to acquisition and loss of colistin resistance were also tracked. Comparative genomic analysis revealed 13 and five colistin resistance determinants in the P5 and P155 lineages, respectively. Lipid A in colistin-resistant mutants was modified through the addition of 4-amino-L-arabinose; this modification was absent in colistin-susceptible revertant strains. Many amino acid substitutions that emerged during the acquisition of colistin resistance were reversed in colistin-susceptible revertants. We demonstrated that evolved colistin resistance inP. aeruginosawas mediated by a complicated regulatory network that likely emerges through diverse genetic alterations. Colistin-resistantP. aeruginosabecame susceptible to the colistin upon its withdrawal because of genetic reversion. The mechanisms through whichP. aeruginosaacquires and loses colistin resistance have implications on the treatment options that can be applied againstP. aeruginosainfections, with respect to improving bactericidal efficacy and preventing further resistance to antibiotics.
Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ
Authors: Zhou Hao , Chen Shun , Wang Mingshu , Jia Renyong , Zhu Dekang , Liu Mafeng , Liu Fei , Yang Qiao , Wu Ying , Sun Kunfeng , Chen Xiaoyue , Jing Bo , Cheng Anchun 
Published: May 2016

AbstractBoth Tembusu virus (TMUV) and goose parvovirus (GPV) are causative agents of goose disease. However, the host immune response of the goose against these two different categories of virus has not been well documented. Here, we compared the clinical symptoms and pathological characteristics, antigen distribution and intensity, and expression of immune-related genes in TMUV- and GPV- infected goose. The immunohistochemistry analysis demonstrated that GPV was primarily located in the liver, lung, small intestine, and rectum, while TMUV was situated in the liver, brain, spleen, and small intestine. The induction of IFNγ and proinflammatory cytokines is highly associated with the distribution profiles of antigen and CD8α+ molecules. The effector function of CD8 T cells may be accomplished by the secretion of IFNγ together with high expression of proinflammatory cytokines such as IL1 and IL6. Remarkably, significant increases in the transcription of immune genes were observed after infection, which suggested that both GPV and TMUV can effectively induce immune response in goose PMBCs. This study will provide fundamental information for goose molecular immunology in defending against pandemic viruses.
Positive impedance humidity sensors via single-component materials
Authors: Qian Jingwen , Peng Zhijian , Shen Zhenguang , Zhao Zengying , Zhang Guoliang , Fu Xiuli 
Published: May 2016

AbstractResistivity-type humidity sensors have been investigated with great interest due to the increasing demands in industry, agriculture and daily life. To date, most of the available humidity sensors have been fabricated based on negative humidity impedance, in which the electrical resistance decreases as the humidity increases, and only several carbon composites have been reported to present positive humidity impedance. However, here we fabricate positive impedance humidity sensors only via single-component WO3−x crystals. The resistance of WO3−x crystal sensors in response to relative humidity could be tuned from a negative to positive one by increasing the compositional x. And it was revealed that the positive humidity impedance was driven by the defects of oxygen vacancy. This result will extend the application field of humidity sensors, because the positive humidity impedance sensors would be more energy-efficient, easier to be miniaturized and electrically safer than their negative counterparts for their lower operation voltages. And we believe that constructing vacancies in semiconducting materials is a universal way to fabricate positive impedance humidity sensors.
Type 3 Diabetes: Cross Talk between Differentially Regulated Proteins of Type 2 Diabetes Mellitus and Alzheimer’s Disease
Authors: Mittal Khyati , Mani Ruchi Jakhmola , Katare Deepshikha Pande 
Published: May 2016

AbstractType 3 Diabetes (T3D) is a neuroendocrine disorder that represents the progression of Type 2 Diabetes Mellitus (T2DM) to Alzheimer’s disease (AD). T3D contributes in the increase of the total load of Alzheimer’s patients worldwide. The protein network based strategies were used for the analysis of protein interactions and hypothesis was derived describing the possible routes of communications among proteins. The hypothesis provides the insight on the probable mechanism of the disease progression for T3D. The current study also suggests that insulin degrading enzyme (IDE) could be the major player which holds the capacity to shift T2DM to T3D by altering metabolic pathways like regulation of beta-cell development, negative regulation of PI3K/AKT pathways and amyloid beta degradation.
Performance improvement of direct torque controlled interior mounted permanent magnet drives by employing a linear combination of current and voltage based flux observers
Authors: Koc Mikail , Sun Tianfu , Wang Jiabin 
Published: May 2016

Flux observers in direct torque controlled (DTC) motor drives are of paramount importance as the drives rely on estimated variables for feedback control. It is well‐known that current‐based (CB) estimations are advantageous at low speeds, whereas voltage‐based (VB) estimations are more accurate at high speeds. Hence, a large number of state‐of‐the‐art DTC drives utilise closed‐loop flux observers in which the CB and VB estimations become dominant at low and high speeds, respectively. However, it has been discovered that the performance and current waveforms with the closed‐loop observers significantly deteriorate at low speeds since the residual error of the VB estimation causes current distortions. In addition, these observers have non‐linear flux transition trajectory resulting in reduced accuracy during transitions. To improve the low speed performance and achieve the linear transition, an alternative combination of the two estimations is proposed. Experimental results on a 10 kW interior mounted permanent magnet machine drive designed for electric vehicle traction applications validate significant improvements on the drive performance.
Thermally induced oxidative growth of copper oxide nanowire on dendritic micropowder and reductive conversion to copper nanowire
Authors: Mirza Gheitaghy Amir , Tabatabaei Seyyede Shahrzad , Saffari Hamid , Zhang Guo Qi 
Published: May 2016

Copper nanowires (NWs) were constructed on a dendritic copper powder precursor by a facile thermal oxidation–reduction method. First, copper oxide NWs were grown from high purity dendritic copper powder by thermal oxidation at 500°C. Next, these oxide NWs were reduced to copper NWs under a hydrogen ﬂow at two different temperatures of 220 and 500°C. Oxide NW diameters distribution was in the range of 50–105 nm with length ranging from 1 to 5 µm while copper NWs were shorter and wider. A time‐dependent study of oxide NWs growth was carried out. The morphology, composition and crystal structure of the resulting products were characterised by X‐ray diffraction and scanning electron microscope. The results indicated that the final product of oxidation process was dispersed cupric oxide (CuO) NW whose density and length increased with time. Furthermore, it was observed that as the reduction temperature increased, the copper NW melted and adhered to the powder surface.
Interactions of a fungal lytic polysaccharide monooxygenase with β-glucan substrates and cellobiose dehydrogenase
Authors: Courtade Gaston , Wimmer Reinhard , Røhr Åsmund K. , Preims Marita , Felice Alfons K. G. , Dimarogona Maria , Vaaje-Kolstad Gustav , Sørlie Morten , Sandgren Mats , Ludwig Roland , Eijsink Vincent G. H. , Aachmann Finn Lillelund 
Published: May 2016

Significance
Copper-dependent lytic polysaccharide monooxygenases (LPMOs) are key players in the enzymatic conversion of biomass. LPMOs catalyze oxidative cleavage of glycosidic bonds in a process involving molecular oxygen and an electron donor, such as cellobiose dehydrogenase (CDH). Using protein NMR and isothermal titration calorimetry we have studied the interactions between a fungal LPMO and three soluble substrates and CDH. The results reveal which areas on the LPMO surface interact with the varying substrates and unambiguously show that both the substrate and CDH bind to a region that is centered around the copper site. The data presented here suggest that electron transfer occurs before substrate binding, providing important new leads for understanding the reaction mechanism of LPMOs.
Evaluating the suitability of mathematical thinking problems for senior high‐school students by including mathematical sense making and global planning
Authors: van Velzen Joke H. 
Published: May 2016

ABSTRACTThe mathematics curriculum often provides for relatively few mathematical thinking problems or non‐routine problems that focus on a deepening of understanding mathematical concepts and the problem‐solving process. To develop such problems, methods are required to evaluate their suitability. The purpose of this preliminary study was to find such an evaluation method by including mathematical sense making and global planning. Eighteen 11th‐grade high‐school students, divided into three groups of three pairs, solved six mathematical thinking problems that included the finding of a numeric solution and the writing of mathematical texts and arguments. Content analysis of the students’ solution procedures provided for three kinds of hierarchically ordered mathematical sense‐making categories. The results showed the expected statistically significant difference between the kinds of problems, though only mathematical sense making enabled the exclusion of the routine problem. The implications for practice are discussed.
Novel metrics to measure coverage in whole exome sequencing datasets reveal local and global non-uniformity
Authors: Wang Qingyu , Shashikant Cooduvalli S. , Jensen Matthew , Altman Naomi S. , Girirajan Santhosh 
Published: May 2016

ABSTRACTWhole Exome Sequencing (WES) is a powerful clinical diagnostic tool for discovering the genetic basis of many diseases. A major shortcoming of WES is uneven coverage of sequence reads over the exome targets contributing to many low coverage regions, which hinders accurate variant calling. In this study, we devised two novel metrics, Cohort Coverage Sparseness (CCS) and Unevenness (UE) Scores for a detailed assessment of the distribution of coverage of sequence reads. Employing these metrics we revealed non-uniformity of coverage and low coverage regions in the WES data generated by three different platforms. This non-uniformity of coverage is both local (coverage of a given exon across different platforms) and global (coverage of all exons across the genome in the given platform). The low coverage regions encompassing functionally important genes were often associated with high GC content, repeat elements and segmental duplications. While a majority of the problems associated with WES are due to the limitations of the capture methods, further refinements in WES technologies have the potential to enhance its clinical applications.
Walking a mile in their shoes: user workarounds in a SAP environment
Authors: Drum Dawna M. , Pernsteiner Aimee J. , Revak Adam 
Published: May 2016

Purpose
The purpose of this paper is to examine workarounds from the perspective of the users of the system, focusing on the outcomes of workarounds and their effect on the quality of accounting information.

Design/methodology/approach
Interviews were conducted with employees in the accounting functions at two international organizations using SAP for their enterprise system. Results were coded by two experienced researchers and one undergraduate student, and included a categorization of the outcomes of workarounds.

Findings
The most striking result is the difference in views of workarounds between the two organizations. While workarounds are discouraged by the accounting function at both organizations, one seeks out workarounds and enforces standards while the other has not yet achieved process standardization. Not surprisingly, users view the workarounds of others as very detrimental to the quality of accounting information, but view their own workarounds as critical to maintain quality.

Practical implications
Organizations should obtain input from system users to document the extent that workarounds are used within the organization to determine the impact on the quality of their financial reporting.

Originality/value
The focus on outcomes of workarounds is an important shift in the literature, and more work is needed to further refine the categories used in this research, particularly for the effect of workarounds on internal controls and information quality. Additionally, this research surfaced a very strong difference in the actions taken to deal with workarounds, which may be due to organizational cultures.
Impact of B2C e-commerce codes of conduct on sales volume: lessons from the Spanish perspective
Authors: Gallego M. Dolores , Bueno Salvador , López-Jiménez David 
Published: May 2016

Purpose
The purpose of this paper is to carry out empirical testing of the relationship between corporate image and corporate satisfaction among business-to-consumer (B2C) e-commerce organizations who possess codes of conduct and study their effects on sales volume. The aim is to measure impacts of both corporate image as well as measure corporate satisfaction on online sales volume increases. In addition, the authors have added to the study the product-type dimension as a moderate variable.

Design/methodology/approach
A structural model is developed and empirically tested through survey data obtained from 127 Spanish companies adhering to a code of conduct.

Findings
The results indicate that adhering to a code of conduct has a positive impact on the increase in sales volumes. The paper identified the main reasons for adhering to a code of conduct, such as offering greater trust to potential consumers as well as improving the firm’s image, prestige and increasing quality and security.

Research limitations/implications
Further variables must be identified that would help to determine the impact on organizations adhering to codes of conduct.

Practical implications
This paper highlights how B2C e-commerce codes of conduct help managers achieve better conditions for competiveness. The findings show how adhering to a code of conduct has a positive impact on increased sales volumes. The results indicate that the reasons for adhering to a code of conduct are the following: it offers greater trust to potential consumers, as well as improves the firm’s image and prestige, and increases purchase quality and security.

Originality/value
This paper contributes to the enrichment of knowledge concerning B2C e-commerce codes of conduct developed a pioneering study.
Improving new product development performance by risk management
Authors: Salavati Mehran , Tuyserkani Milad , Mousavi Seyyede Anahita , Falahi Nafiseh , Abdi Farshid 
Published: May 2016

PurposeThe principal aim of this study is to investigate the relationship between technological, marketing, organizational and commercialization risk management on new product development (NPD) performance.Design/methodology/approachBased on questionnaire, the data were collected from a sample of general automotive industry in Iran. Based on theoretical considerations, a model was proposed and descriptive statistic and hierarchical regression were used to measure the relationship between risk management factors and NPD performance.FindingsData analysis revealed that if organization can amplify their knowledge and information about risk and main factors that affect NPD process, not only can they do their work better but can also increase their ability to predict future happenings that affect performance.Research limitations/implicationsFirst, due to the relatively small sample size, caution should be exercised when interpreting the results. Second, the data were collected from automotive producer in Iran, which may restrict to some extent generalizability of the findings.Practical implicationsThe results suggest that managers should consider more attention to risk management. If managers spread the risk management in all aspects of the NPD project, total performance will be increased and it can develop the probability of NPD success. Also organizations should perform great market research due to best commercialization.Originality/valuePast researches have presented complete information about NPD process. But identifying and considering the effect of the risk management parameters that are connected to the NPD process were the main thrusts to perform the study. In this paper, based on past research about risk management of NPD, the extra aspect of process that can improve total performance of NPD has been examined.
IAS 39, income smoothing, and pro-cyclicality: evidence from Hong Kong banks
Authors: Abdul Adzis Azira , Tripe David W.L. , Dunmore Paul 
Published: May 2016

Purpose
The purpose of this study is to investigate the impact of International Accounting Standard 39 (IAS 39) on income-smoothing activities and pro-cyclical behavior through loan loss provisions using a sample of Hong Kong banks.

Design/methodology/approach
Fixed effects estimator is used, and the analysis covers the period from 2000 to 2009.

Findings
The results suggest that Hong Kong banks engage less in income-smoothing activity after they comply with the IAS 39. No evidence supports loan loss provisions of Hong Kong banks exhibiting more pro-cyclical behavior after IAS 39 adoption.

Research limitations/implications
Compliance with IAS 39 should improve the quality of bank financial reporting. The reduction in income-smoothing activities among Hong Kong banks after IAS 39 adoption fairly supports the effectiveness of International Financial Reporting Standard (IFRS) and countries that have yet to comply with IFRS may take action to apply the standards. Bank regulators should take pro-active action in addressing the issue of pro-cyclicality of loan loss provisions, as IAS 39 focuses more on improving the financial information quality, while pro-cyclicality is associated with the economic cycles.

Originality/value
Hong Kong banking industry is unique, as it was among the first IFRS adopters in the East Asia region and it has its own legal framework for developing accounting standards. The results of this study are expected to shed some light on the effects of IAS 39 adoption on income smoothing and pro-cyclicality of banks in the East Asia region, where the accounting cultural value dimensions and institutional structures are different than that of European countries.