bluuebunny/crossref_metadata_2025_split huggingface dataset:
Critical period for first language acquisition may be shorter in autistic children than in typically developing children
Authors: Vyshedskiy Andrey , Marsiglio Allegra , Batham Sahil , Tagliavia Alessandro , Venkatesh Rohan , Tarakbay Anel , Mundhia Sagar , Urs Samarth , Khokhlovich Edward , Pinsky Eugene 
Published: October 2024

Abstract

The goal of this study was to differentiate between two hypotheses regarding syntactic-language comprehension deficits in autistic adults. One hypothesis suggests a persistent, age-independent barrier, such as sound hypersensitivity or social avoidance, which may hinder acquisition of syntax throughout life. Another hypothesis proposes an age-dependent factor, such as a shortened critical period for language acquisition. These hypotheses predict distinct trajectories for language learning-rates. The first hypothesis expects autistic individuals to consistently exhibit a slower learning-rate compared to neurotypical individuals across all ages. In contrast, the second hypothesis predicts that autistic individuals will initially acquire language at a rate comparable to their neurotypical peers but will experience an earlier decline in learning-rate. To test these predictions, we analyzed language learning-rates in 15,183 autistic and 138 neurotypical individuals, 2 to 22 years-of-age. At age 2, both groups showed comparable learning-rates. In neurotypical individuals, this rate remained stable from ages 2 to 7. However, in autistic individuals, the learning-rate began to decline exponentially starting as early as 2.3 years, with an earlier onset of decline observed in those with more severe autism. These findings strongly support the second hypothesis, indicating that language deficits in autism may be caused by a shortened critical period.
A SPR based fiber optic sensor for the development of Internet of Things (IoT) Centered Technologies
Authors: Aktar Mst Nargis , Basak Nilanjana , Biswas Shuvo , Abdullah Hasan , Uddin Muhammad Shahin 
Published: October 2024

Abstract

Photonic crystal fiber (PCF) is the crucial component of optical fiber. Today, PCF has sparked widespread interest because of its potential in sensor systems, photonic devices, and communication. However, the features of technologies are updating day by day, and to keep up with the latest features, optical fiber sensors are a key issue for the Internet of Things (IoT) technologies. The primary goal of the offered project is to design an optical sensor for IoT-compatible devices with maximum sensitivity and minimum loss. Therefore, this project presents a unique circular-shape PCF sensor based on surface plasmon resonance (SPR) for evaluating analyte refractive index (RI). With the utilization of the finite element approach, sensing capabilities of the proposed structure have been examined via numerical simulations incorporating an iterative optimization. However, in this configuration, several capillaries are collected and conducted to yield a circular-shape silica structure, then coating the flattened layer with a gold (Au) material. The gold material obtains the SPR pulse in the PCF’s broadcast spectrum. In this structure, the operating wavelength range of 0.94 − 0.62 µm yields efficient results. All the parameters are numerically simulated for the analyte, with RI = 1.34–1.37. After the simulation and formal analysis, the offered sensor gives the highest wavelength sensitivity response of 18403.59 nm/RIU and a minimum loss peak of 193.87 dB/cm. We believe the structural design can also be an appropriate candidate for biological and organic detection, as well as other IoT-based applications.
Exploring the Psychometric Properties of the Life Orientation Test-Revised Among Multiple Sclerosis Patients, Insights into Optimism
Authors: Yunier Broche-Pérez , M. Jiménez-Morales Rodneys , D. Díaz-Guerra Diego 
Published: October 2024

Abstract

Introduction: Optimism, defined as the tendency to expect positive outcomes and view challenges as manageable, plays a vital role in the lives of individuals with chronic illnesses, including multiple sclerosis. Given its potential benefits, measuring optimism through validated tools like the Life Orientation Test-Revised (LOT-R) is essential for both research and clinical practice.
Objective: This study aims to validate the LOT-R within a sample of MS patients, assessing its psychometric properties and exploring its potential utility in clinical settings.
Methods: This study included 179 patients with Relapsing-Remitting Multiple Sclerosis (PwMS). Confirmatory factor analysis (CFA) was performed to assess the factor structure of the LOT-R. Model fits were evaluated by the χ2/df value, comparative fit index (CFI), and root mean square error of approximation (RMSEA).
Results: The LOT-R showed good psychometric characteristics in our sample of PwMS. The CFA's two-factor model (optimism/pessimism) yielded a good model fit. Cronbach's alpha and McDonald’s omega were acceptable. Optimism was significantly correlated with quality-of-life scores, resilience, and fear of relapse scale.
Conclusions: The LOT-10 has satisfactory psychometric properties and is suitable for measuring dispositional optimism in Spanish-speaking patients with multiple sclerosis.
Hydrophilic and strength-softening characteristics of clay-rich rocks
Authors: Han Zongfang , Bu Mohua , Li Yuqiong , He Manchao 
Published: October 2024

Abstract

To better understand the mechanism of the strength weakening process of clay-rich rocks after interacting with water, the relationship between mineral composition, microscopic pore structure and gaseous water adsorption characteristics of clay-rich rocks was comprehensively analyzed by using X-ray diffraction (XRD), scanning electron microscopy (SEM) and mercury injection test. The results show a positive correlation between water absorption and both clay minerals and montmorillonite (MMT) content. The more complex the microporous structure is, the more pores in the diameter of 0.2 ~ 2µm accumulated, and the worse the water absorption capacity obtained. The innovation of this study lies in quantitatively calculating the weight coefficients of influencing factors, highlighting the contribution of pore structure and hydrophilic mineral composition to the strength of clay-rich rocks samples after water absorption, which was not covered in previous studies. The results show that the weight coefficients are fractal dimension of pores, effective porosity and clay mineral content in order. The clay minerals content is negatively correlated with the strength of the clay rocks after water absorption, that is, the presence of MMT is the key influencing factor of strength softening in this study, and the paper concludes with the results of molecular dynamics (MD) simulation to illustrate more intuitively the effect of hydrated MMT on strength. The research results provide a new insight to better understand the strength softening caused by water sensitivity of clay-rich rocks and to establish the correlation between the macroscopic mechanical behavior and the micromechanical mechanism.
Re-examining the Optimal Extent of Lymph Node Dissection for Colon Cancer using the Lymphadenectomy Index
Authors: Marunaka Yuta , Kiuchi Jun , Kuriu Yoshiaki , Arita Tomohiro , Shimizu Hiroki , Nanishi Kenji , Imamura Taisuke , Ohashi Takuma , Konishi Hirotaka , Yamamoto Yusuke , Morimura Ryo , Shiozaki Atsushi , Ikoma Hisashi , Kubota Takeshi , Fujiwara Hitoshi , Otsuji Eigo 
Published: October 2024

Abstract

The optimal extent of lymph node dissection in colon cancer surgery is specified in guidelines based on the results of past analyses. However, with advances in surgical techniques and multidisciplinary treatments, the clinical significance of dissecting each lymph node may change. In this study, we re-examined the optimal dissection range in each colon cancer localization. We retrospectively analyzed 788 cases of T1-T4 colon cancer who underwent radical resection between 2008 and 2018 at our hospital, and evaluated the Lymphadenectomy Index. No metastases to the main lymph node were found in T1 cases. In T2 cases, dissection effect to the main lymph node were observed in cases with tumors localized in the ascending colon and left side of the transverse colon. For tumors localized in the cecum, dissection was effective for lymph nodes in nodal station 213, in the right side of the transverse colon in station 211, in the descending colon in station 221, and in the sigmoid colon in station 231. These lymph nodes could have been considered out of scope for dissection if the Japanese guidelines were followed. In these cases, the extent of lymph node dissection should be carefully considered on a case-by-case basis.
Disulfidptosis-associated ferroptosis gene BCAT2 regulates c-MYC expression and promotes the malignant progression of epithelial ovarian cancer cells by interacting with GYS1
Authors: Tang Zhongyun , Ma Chao , Liu Chongdong 
Published: October 2024

Abstract

Purpose
Epithelial ovarian cancer (EOC) is a prevalent gynecological malignancy with a notoriously poor prognosis. Recent oncological research has highlighted ferroptosis and disulfidptosis as novel forms of cell death with potential therapeutic implications. However, the relationship between these processes and their role in EOC remains poorly understood.
Methods
Through comprehensive bioinformatics analyses, we identified six disulfidptosis-related ferroptosis genes in EOC samples. A prognostic model was developed utilizing these genes to assess their predictive power for EOC survival prognosis. We further investigated the dual role of BCAT2 in regulating ferroptosis and modulating GYS1, which is implicated in disulfidptosis. The influence of BCAT2 on cell proliferation, migration, and invasion was examined through its regulation of c-MYC and its impact on G2M phase and EMT-related proteins.
Results
Our findings reveal that BCAT2 plays a pivotal role in both ferroptosis and disulfidptosis in EOC. It not only regulates ferroptosis but also modulates GYS1, thereby influencing disulfidptosis. We observed a strong correlation between BCAT2 and GYS1 expression. Additionally, BCAT2's interaction with GYS1 extends its influence beyond ferroptosis, significantly disrupting disulfidptosis as well.
Conclusion
This study provides valuable insights into the complex interplay between disulfidptosis and ferroptosis in EOC, our findings underscore the potential of targeting BCAT2 and GYS1 as a therapeutic strategy for EOC.
Strategies for occupational recovery processes in individuals with bipolar disorder type I: a qualitative study.
Authors: Koene Juul , Maassen Eva , Lang Natasja van , Stel Jaap van der , Kupka Ralph , Weeghel Jaap van , Kroon Hans 
Published: October 2024

Abstract

Background. Employment is a challenging life domain for individuals with bipolar disorder (BD), illustrated by high unemployment numbers and various experienced problems within the workplace. While regaining a satisfactory level of occupational functioning is an important aspect of recovery, there is little qualitative research exploring occupational recovery in individuals with BD. The aim of this qualitative study was to gain insight in the strategies of patients and peer support workers with BD type I (BD-I) regarding their occupational functioning. Our second aim was to construct a conceptual model based on these experiences to provide further understanding of this aspect of recovery. In our study, inspired by Glaser and Strauss' constructivist Grounded Theory, we interviewed 21 patients and 15 peer support workers with BD-I about their occupational functioning experiences and strategies for dealing with difficulties, as well as their occupational recovery processes. We analysed the data through open, axial, and selective coding.
Results. The results showed one key theme and three categories of strategies implemented by individuals with BD-I: (1) organizing work, (2) self-regulatory actions in relation to employment, and (3) getting support. Based on the narratives of participants, we constructed a conceptual model to get a clearer understanding of their occupational recovery processes to manage challenges they encounter in their workplace.
Conclusion. The narratives of individuals with BD-I show that two types of recovery can be experienced in relation to work: recovery after an acute mood episode and a longer lasting recovery process that is intertwined with several aspects of daily working life. Strategies that individuals develop to regulate their work behaviour and their work environment aid in this recovery process.
Geospatial analysis of individuals with suspected diagnosis of leprosy and positive qPCR in an endemic region of Minas Gerais
Authors: Pinheiro Marcos Daniel Silva , Oliveira Nathan Guilherme , Rios Vladmir Machado , Takeishi Lucas Kenji , Batista Lavínia Cássia Ferreira , Branco Alexandre Castelo , Fairley Jessica K. , Oliveira Lorena Bruna Pereira , Baptista Ida Maria Foschiane Dias , Fraga Lucia Alves Oliveira 
Published: October 2024

Abstract

Background: to evaluate the geographic distribution of individuals with a suspected leprosy diagnosis (SLD) presenting a positive qPCR test residing in Governador Valadares/MG.
Methods: Between 2017 and 2021, dermal scraping samples were collected from the earlobes, elbows, and lesions of 200 individuals with SLD examined in CREDEN-PES. A geospatial analysis estimated the coverage of health units.
Results: It was found that 108 (54.0%) of the participants were female, with an average age of 55 years, and the majority lived in the urban area of the municipality. The qPCR test was positive in 94 (47.0%) samples evaluated, of which 49 (52.1%) were from female individuals and 45 (47.9%) were males. A heterogeneous distribution of individuals who presented a positive qPCR test in the municipality was observed. However, in the sectors with the highest number of individuals with SLD and positive in the qPCR test, there was a predominance of female, literate, black, or mixed-race residents. Furthermore, 58.8% of individuals with SLD and positive in the qPCR test did not have coverage at a health facility.
Conclusion: The lack of health unit coverage in places with more SLD and qPCR-positive individuals indicates the need to expand this coverage and implement active search, diagnostic suspicion, and treating leprosy in Primary Care. This would facilitate user access to the nearest.
Health service, thus making Secondary Care (CREDEN-PES) available to perform its role as a Specialized unit.
Measurement Uncertainty and Validation for Quantification of Marijuana Metabolite: (−)-11-nor-9-Carboxy-Δ9-THC in Human Urine by GC-MS/MS
Authors: Maurya Ashok Kumar , Sahu Puran Lal , Sahu Kapendra 
Published: October 2024

Background:
Since the International Olympic Committee (IOC) was established,
sports doping control analyses have revealed a high rate of positive cases for cannabinoids.
Cannabinoids were banned in all sports where they were used in-competition as per the Prohibited
List published annually. Further, it was also included in the threshold drug category. Consequently,
developing a reliable method for urine Cannabinoids metabolite quantification plays
a pivotal role in sports dope testing.

Objective:
This work aimed to develop and validate a reliable, cost-effective, robust gas
chromatography-tandem mass spectrometry method for detecting (−)-11-nor-9-Carboxy-Δ9-
THC component in human urine samples, in compliance with ICH and WADA guidelines.

Methods:
The sample preparation was done by enzymatic hydrolysis for deconjugation, further
proceeded with solid phase extraction (SPE), liquid-liquid extraction (LLE), and using an
XAD2 column, and N-methyl trimethylsilyl trifluoroacetamide (MSTFA) for derivatization.

Results:
The linearity was obtained in a range of 50–300 ng/mL, and the correlation coefficient
was found to be higher than 0.99. Throughout the entire validation study, the difference in
Retention Time (RT) for the analyte, including the Internal Standard (IS), was shown to be less
than 1.0%. The quantification limit (LOQ) was calculated at a level of 50 ng/mL in human
urine samples for the 3 most abundant ion transitions. The detection limit (LOD) was
established at 4 ng/mL.

Conclusion:
The accuracy, precision, linearity, recovery, quantification limit, and selectivity
by GC-MS/MS technique were found acceptable and well satisfactory while following the ICH
guidelines. The developed method has been proven to be fit for purpose in accordance with the
enforced Guidelines of WADA.
Identification of Phytoconstituents from Natural Product Database as SIRT2 Inhibitors for Potential Role in Alzheimer’s Disease: An In-Silico Screening
Authors: Kumar Hitesh , Datusalia Ashok Kumar , Kumar Anoop , Khatik Gopal L. 
Published: October 2024

Aim:
We aimed to conduct in silico screening of the potential phytoconstituent from a
natural product database to find SIRT2 inhibitors.

Background:
Alzheimer's disease (AD) is the most prevalent type of dementia, characterized by
behavioral and mental symptoms as well as a progressive loss of cognitive ability. Since SIRT2
may be detrimental to neurological illnesses, it is a prime target for research into SIRT2 inhibitors.

Objective:
To identify the SIRT2 inhibitors and their role in AD.

Methods:
We have utilized NPAtlas database and screened using pharmacophore-based virtual
screening, molecular docking, and simulation. The Natural Products Atlas provides unrestricted access
to various natural products derived from bacteria and fungi, allowing researchers to investigate
and visualize the extensive chemical diversity in the natural world.

Results:
From in silico screening data, we have found phytoconstituents that could function as
SIRT2 inhibitors. Six phytoconstituents were identified using pharmacophore-based virtual screening.
According to molecular docking, Kurasoin B outperformed the reference molecule regarding
binding energy. Kurasoin B exhibited a binding affinity of -12.543 kcal/mol, whereas the binding
affinity of the reference molecule was -12.089 kcal/mol. The Kurasoin B complex with SIRT2 was
determined to be stable throughout the simulation by performing MD simulation, with an RMSD of
2.88 (Å), whereas the reference and free protein displayed RMSDs of 3.74 and 4.70 (Å), respectively.

Conclusion:
In silico studies and data analysis, suggest that Kurasoin B may be able to suppress
the SIRT2 protein for managing AD.
Synergistic Action of Thymol-citral is Associated with Cell Cycle Arrest and Intracellular ROS Generation in A549 Cells
Authors: Balan Devasahayam Jaya , Kiruthiga Chandramohan , Jeyakumar Mahalingam , Devi Kasi Pandima 
Published: October 2024

Objective:
NSCLC is the predominant form of lung cancer, often exhibiting resistance
to chemotherapy. Thymol and citral have shown promise as anticancer agents in different cancer
cell lines but have not been evaluated in combination against NSCLC. Hence, we planned to investigate
the anticancer effect of thymol-citral combination and explore its mechanisms of action
against A549 cells.

Methods:
A549 cells were exposed to varying concentrations of thymol and citral, alone and in
combination. Cell proliferation, plasma membrane integrity, apoptotic markers, reactive oxygen
species (ROS) levels, cell cycle distribution, senescence induction, and migration potential were
assessed. Additionally, in vitro safety was evaluated in human bronchial epithelial cells (HBECs)
and human red blood cells (RBCs).

Results:
Thymol and citral showed synergistic action against A549 cells, with a CI value of 0.75.
After 24 h, they induced apoptosis, caused G0/G1 phase arrest, and increased ROS levels, suggesting
oxidative stress as the mechanism. This combination also induced cell senescence, significantly
inhibited A549 cell migration, and was non-toxic to human RBCs and HBECs.

Conclusion:
Overall, the thymol-citral synergistic combination was found to be a safe and effective
therapy option for non-small cell lung cancer.
Uso de un digestato elaborado a partir de residuos orgánicos urbanos: evaluación de su efecto en el suelo y en un cultivo de Raphanus sativus L.
Authors: Rodriguez  Aristizabal Monica Alejandra , Yaya Lancheros Mary Luz , Cifuentes Olaya Brigitte Alexandra , Escobar Rojas Aidee Vanessa 
Published: October 2024

Colombia, considerado un país de vocación agrícola y que históricamente ha dependido de productos agroquímicos para el acondicionamiento fisicoquímico, la fertilización y nutrición del suelo, así como para el control de plagas en los cultivos, no es ajeno a problemáticas como la degradación del suelo, la contaminación ambiental y la pérdida de biodiversidad como consecuencia del uso de estos productos, poniendo en riesgo la seguridad alimentaria. Como alternativa, el uso de bioinsumos a base de microorganismos ha demostrado beneficios en el rendimiento y la sanidad de los cultivos. En ese contexto, este trabajo tuvo como objetivo evaluar el uso de un digestato elaborado con residuos orgánicos urbanos como acondicionador fisicoquímico del suelo. La metodología incluyó la caracterización fisicoquímica del digestato, análisis microbiológicos, pruebas de inocuidad, para detectar la presencia de Salmonella spp (Lignières, 1900) y huevos de helmintos, ensayos de fitotoxicidad en la germinación y un bioensayo con plántulas de Raphanus sativus L. Los resultados fisicoquímicos indicaron que el digestato es un líquido altamente salino y, en la aproximación a la diversidad microbiológica, se detectaron bacterias amilolíticas. En cuanto a la fitotoxicidad se demostró que el digestato no contiene sustancias que inhiban la germinación, según los análisis estadísticos de Bonferroni y Kruskal-Wallis. Sin embargo, el bioensayo mostró que el digestato no tuvo un impacto significativo en el acondicionamiento del suelo, ya que no se observó mejora en la altura, número de hojas o peso seco de las plántulas. De acuerdo con estos resultados, se concluyó que el digestato obtenido requiere ajustes en el proceso de elaboración. Se recomienda su uso en cultivos con ciclos fenológicos más largos, además de incluir la adición de inóculos microbianos que mejoren su efectividad, así como la mejora de sus propiedades, lo cual podría aumentar su potencial como biofertilizante.
Profil Penggunaan Granulocyte Colony Stimulating Factor (G-Csf) Pada Pasien Kanker Dengan Neutropenia di RSUP Dr. Kariadi Semarang
Authors: Ningsih Diana Rachma , Murwanti Retno , Nugroho Agung Endro 
Published: October 2024

Kejadian neutropenia pada pasien kanker disebabkan 90% akibat kemoterapi. Penundaan pengatasan neutropenia dapat menyebabkan risiko terjadinya febril neutropenia sehingga menyebabkan tingginya mortalitas. Pasien kanker dengan neutropenia dapat diberikan terapi granulocyte colony stimulating factor (G-CSF), yang dapat mengurangi kejadian febril neutropenia, lama perawatan, menurunkan jumlah angka kesakitan dan kematian pada pasien kanker. Tujuan dari penelitian ini adalah mengetahui profil penggunaan G-CSF pada pasien kanker dengan neutropenia. G-CSF yang digunakan adalah Filgrastrim dan Lenograstrim. Penelitian ini menggunakan Retrospective Cohort Study dengan observational analitik yang dilaksanakan di RSUP Dr. Kariadi Semarang. Data penelitian dikumpulkan secara retrospektif menggunakan catatan medik pada periode 2019-2022. Sampel penelitian diperoleh 56 subyek yang memenuhi kriteria inklusi. Berdasarkan hasil penelitian pasien kanker dengan neutropenia lebih banyak terjadi pada pasien kanker non hematologi sebesar (62,5%) dengan rentang usia paling banyak terjadi pada pada 18-60 tahun (78,6%). Grade neutropenia yang diamali pasien paling banyak yaitu neutropenia severe (55,3%), sedangkan pada neutropenia moderatre sejumlah (32,2%), dan neutropenia mild sebesar (12,5%). Profil penggunaan G-CSF pada pasien kanker dengan neutropenia di RSUP Dr. Kariadi yang diteliti yaitu Filgrastrim dan Lenograstrim. Pasien yang diberikan Filgrastrim sejumlah (73,2%) dan yang diberikan Lenograstrim sejumlah (26,8%).