bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biodistribution and Targeting of Anti-5T4 Antibody–Drug Conjugate Using Fluorescence Molecular Tomography
Authors: Giddabasappa Anand , Gupta Vijay R. , Norberg Rand , Gupta Parul , Spilker Mary E. , Wentland Joann , Rago Brian , Eswaraka Jeetendra , Leal Mauricio , Sapra Puja 
Published: July 2016

Abstract
Understanding a drug's whole-body biodistribution and tumor targeting can provide important information regarding efficacy, safety, and dosing parameters. Current methods to evaluate biodistribution include in vivo imaging technologies like positron electron tomography and single-photon emission computed tomography or ex vivo quantitation of drug concentrations in tissues using autoradiography and standard biochemical assays. These methods use radioactive compounds or are cumbersome and do not give whole-body information. Here, for the first time, we show the utility of fluorescence molecular tomography (FMT) imaging to determine the biodistribution and targeting of an antibody–drug conjugate (ADC). An anti–5T4-antibody (5T4-Ab) and 5T4-ADC were conjugated with a near-infrared (NIR) fluorophore VivoTag 680XL (VT680). Both conjugated compounds were stable as determined by SEC-HPLC and plasma stability studies. Flow cytometry and fluorescence microscopy studies showed that VT680-conjugated 5T4-ADC specifically bound 5T4-expressing cells in vitro and also exhibited a similar cytotoxicity profile as the unconjugated 5T4-ADC. In vivo biodistribution and tumor targeting in an H1975 subcutaneous xenograft model demonstrated no significant differences between accumulation of VT680-conjugated 5T4-Ab or 5T4-ADC in either normal tissues or tumor. In addition, quantitation of heart signal from FMT imaging showed good correlation with the plasma pharmacokinetic profile suggesting that it (heart FMT imaging) may be a surrogate for plasma drug clearance. These results demonstrate that conjugation of VT680 to 5T4-Ab or 5T4-ADC does not change the behavior of native biologic, and FMT imaging can be a useful tool to understand biodistribution and tumor-targeting kinetics of antibodies, ADCs, and other biologics. Mol Cancer Ther; 15(10); 2530–40. ©2016 AACR.
Integrin-αIIbβ3-mediated outside-in signalling activates a negative feedback pathway to suppress platelet activation
Authors: Dai Baiyun , Wu Peng , Xue Feng , Yang Renchi , Yu Ziqiang , Dai Kesheng , Ruan Changgeng , Liu Gang , Newman Peter J. , Gao Cunji 
Published: July 2016

SummaryIntegrin-αIIbβ3-mediated outside-in signalling is widely accepted as an amplifier of platelet activation; accumulating evidence suggests that outside-in signalling can, under certain conditions, also function as an inhibitor of platelet activation. The role of integrin-αIIbβ3-mediated outside-in signalling in platelet activation is disputable. We employed flow cytometry, aggregometry, immunoprecipitation, and immunoblotting to investigate the role of integrin-αIIbβ3-mediated outside-in signalling in platelet activation. Integrin αIIbβ3 inhibition enhances agonist-induced platelet ATP secretion. Human platelets lacking expression of αIIbβ3 exhibited more platelet ATP secretion than their wild-type counterparts. Moreover, integrin-αIIbβ3-mediated outside-in signals activate SHIP-1, which in turn mediates p-Akt dep-hosphorylation, leading to inactivation of PI3K/Akt signalling. Furthermore, 3AC (SHIP-1 inhibitor) inhibits platelet disaggregation, and promotes platelet ATP secretion. Upon ADP stimulation, Talin is recruited to αIIbβ3, and it is dissociated from αIIbβ3 when platelets disaggregate. In addition, treatment with RUC2, an inhibitor of αIIbβ3, which blocks αIIbβ3-mediated outside-in signalling, can markedly prevent the dissociation of talin from integrin. SHIP1 Inhibitor 3AC inhibits the dissociation of talin from integrin-β3. These results suggest that integrin-αIIbβ3-mediated outside-in signalling can serve as a brake to restrict unnecessary platelet activation by activated SHIP-1, which mediated the disassociation of talin from β3, leading to integrin inactivation and blocking of PI3K/Akt signalling to restrict platelet ATP secretion.
Treatment with the GPR55 antagonist CID16020046 increases neutrophil activation in mouse atherogenesis
Authors: Bondarenko Alexander I. , Lenglet Sébastien , Burger Fabienne , Piscitelli Fabiana , Carbone Federico , Roth Aline , Liberale Luca , Dallegri Franco , Brandt Karim J. , Fraga-Silva Rodrigo A. , Stergiopulos Nikolaos , Montecucco Fabrizio , Di Marzo Vincenzo , Mach François 
Published: July 2016

SummaryEndocannabinoids modulate atherogenesis by triggering different receptors. Recently, orphan G protein-coupled receptors (GPRs) were suggested to be activated by endocannabinoids, possibly regulating vasorelaxation. Here, we investigated whether GPR55 antagonism with CID16020046 would impact on atherosclerotic size and inflammation in two mouse models of early and more advanced atherogenesis. Eleven-week old ApoE−/− mice were fed either a normal diet ([ND] for 16 weeks) or a high-cholesterol diet ([HD] for 11 weeks), resulting in different degrees of hypercholesterolaemia and size of atherosclerosis. CID16020046 (0.5 mg/kg) or vehicle were intraperitoneally administrated five times per week in the last three weeks before euthanasia. Treatment with CID1602004 was well-tolerated, but failed to affect atherosclerotic plaque and necrotic core size, fibrous cap thickness, macrophage and smooth muscle cell content as well as Th cell polarisation. In ND mice, treatment with CID1602004 was associated with increased chemokine production, neutrophil and MMP-9 intraplaque content as well as reduced collagen as compared to vehicletreated animals. In HD mice, CID1602004 increased intraplaque MMP-9 and abrogated collagen content without affecting neutrophils. In vitro, serum from CID1602004-treated ND mice increased mouse neutrophil chemotaxis towards CXCL2 as compared to serum from vehicletreated animals. CID1602004 dose-dependently induced neutrophil degranulation that was reverted by co-incubation with the GPR55 agonist Abn-CBD. In supernatants from degranulation experiments, increased levels of the endocannabinoid and putative GPR55 ligand anandamide (AEA) were found, suggesting its possible autocrine control of neutrophil activity. These results indicate that GPR55 is critical for the negative control of neutrophil activation in different phases of atherogenesis.Supplementary Material to this article is available online at www.thrombosis-online.com.
An Assessment of Future Southern Hemisphere Blocking Using CMIP5 Projections from Four GCMs
Authors: Parsons Simon , Renwick James A. , McDonald Adrian J. 
Published: July 2016

AbstractThis study is concerned with blocking events (BEs) in the Southern Hemisphere (SH), their past variability, and future projections. ERA-Interim (ERA-I) is used to compare the historical output from four general circulation models (GCMs) from phase 5 of the Coupled Model Intercomparison Project (CMIP5); the output of the representative concentration pathway 4.5 and 8.5 (RCP4.5 and RCP8.5) projections are also examined. ERA-I shows that the higher latitudes of the South Pacific Ocean (SPO) are the main blocking region, with blocking occurring predominantly in winter. The CMIP5 historical simulations also agree well with ERA-I for annual and seasonal BE locations and frequencies. A reduction in BEs is observed in the SPO in the 2071–2100 period in the RCP4.5 projections, and this is more pronounced for the RCP8.5 projections and occurs predominantly during the spring and summer seasons. Preliminary investigations imply that the southern annular mode (SAM) is negatively correlated with blocking activity in the SPO in all seasons in the reanalysis. This negative correlation is also observed in the GCM historical output. However, in the RCP projections this correlation is reduced in three of the four models during summer, suggesting that SAM may be less influential in summertime blocking in the future.
Existence of renal dysfunction in diabetics undergoing coronary artery bypass
Authors: Ali Taimur Asif , Salahuddin Urooj , Shoukat Arfeel , Shahzad Noman , Naeem Syed Saad , Dar Mudassir Iqbal , Fatimi Saulat Hasnain 
Published: July 2016

Introduction The effect of diabetes mellitus on morbidity and mortality in patients undergoing coronary artery bypass grafting has remained uncertain, and conflicting conclusions have been reported in clinical trials. Evidence suggests that coronary artery bypass in patients with diabetes mellitus carries a higher risk of stroke, renal failure, perioperative complications, and sternal wound infection. This study evaluated the frequency of acute renal dysfunction after coronary artery bypass in diabetic patients, and the associated risk factors. Method This cross-sectional observational study included 135 patients with diabetes (111 males and 24 females with a mean age 51 years and a body mass index of 27.44 kg m−2), who underwent elective coronary artery bypass from March 2015 to October 2015. Data were collected prospectively in 2 tertiary care centers. Renal dysfunction was assessed by serum creatinine levels preoperatively and at 24 and 48 h postoperatively. Results Fifteen percent of patients were found to have postoperative renal dysfunction. Univariate analysis revealed that patients with increased serum creatinine preoperatively were at greater risk of developing renal dysfunction after coronary artery bypass ( p = 0.00). On multivariable binary logistic regression analysis, preoperative serum creatinine level was the only independent predictor of postoperative renal dysfunction; age, body mass index, dyslipidemia, hypertension, cardiopulmonary bypass time, and aortic crossclamp time showed no significant association. Conclusion Diabetic patients with increased serum creatinine preoperatively are at greater risk of kidney damage postoperatively; therefore, these patients should be monitored and treated critically in the perioperative period.
Development and validation of the Taiwan Children’s Health Literacy Test
Authors: Liu Chieh-Hsing , Liao Li-Ling , Cheng Chi-Chia J. , Chang Tzu-Chau 
Published: July 2016

Background: Currently, health literacy (HL) worldwide is measured primarily among adults from a medical perspective. A children’s HL test from a health promotion perspective is needed. This study develops the Taiwan Children’s Health Literacy Test (TCHL) targeted at Taiwanese sixth-graders. Methods: HL indicators were identified through interviews of sixth-grade teachers, primary caregivers, and sixth-graders. They were used to develop a 25-item online test, which was finalized after pretesting and administered to a stratified random sample of 671 students across 29 sixth-grade classes. Results: The response rate was 87.2% (585 participants). Cronbach’s α was 0.60–0.78 for the HL subtests and 0.79 for the overall HL test. Correlation coefficients among subtests ranged from 0.49 to 0.87. The average proportion correct was 0.80 for overall HL. In item response theory analysis, the difficulty level ranged from −2.77 to −0.40, while the degree of discrimination was 0.44–2.65. Item information peaked when the ability value was approximately −1.8. Furthermore, HL scores had a significant positive correlation with health behaviors. Conclusions: Overall, the test was found to be reliable and valid, with a low level of difficulty for basic HL ability. The present results can be used as a reference by government health education units to investigate national HL in children.
Fluorescent Gelators for Detection of Explosives
Authors: Hanabusa Kenji , Takata Shingo , Fujisaki Masafumi , Nomura Yasushi , Suzuki Masahiro 
Published: July 2016

Abstract
Carbazole-, quinoline-, benzothiazole-, and stilbene-containing fluorescent gelators are synthesized by connecting gelation-driving segments, and their gelation abilities are studied with 13 solvents. Fibrous thin-layer films are prepared on quartz plates from the solutions or gels, and they are studied as chemosensors for explosives. Fluorescence quenching of the films upon exposure to saturated TNT or RDX vapor is used to evaluate the abilities of the films to detect explosives. The relationship between the thickness of the thin-layer film and the quenching efficiency upon exposure to TNT is studied. The morphologies of the thin-layer films are observed by dynamic force mode scanning probe microscopy and discussed with regard to their fluorescence quenching. The interactions among chromophores in the gels, thin-layer films, and solutions are studied by variable-temperature spectroscopy. The mechanism of TNT detection is discussed from the viewpoint of the HOMO and LUMO energy levels.
Preoperative fasting for elective surgery in a regional hospital in Oman
Authors: Abdullah Al Maqbali Mohammed 
Published: July 2016

Background: A fasting period before anesthesia is necessary to avoid the aspiration of stomach contents, which can be threatening to the patient's life. Guidelines from professional societies in the USA and UK recommend that healthy patients fast for 6 hours from solid food and 2 hours from liquids. Despite this, many institutions still practice nil-by-mouth after midnight. This can affect the patient's recovery after surgery, and increase the length of stay in hospital. The aim of this study was to assess the duration of fasting before elective surgery on the part of adult patients. Methods: A prospective study was conducted to identify the fasting time and complications among surgical patients undergoing elective surgery over a 4-month period. The patients were asked for preoperative fasting times, and the complication. The demographic data were taken from the patients' files. Result: A total of 169 patients were included in the study, 88 male and 81 female. The minimum and maximum fasting hours with regard to food were 7 hours and 19 hours, respectively; all the patients fasted from food for longer than the recommended time. The minimum and maximum fasting hours for fluids were 4 hours and 19 hours, respectively; all the patients fasted from fluid for longer than the recommended time. Conclusion: Most of the patients fasted from food and fluids for more than the time recommended by the American Society of Anaesthesiologists, the Royal College of Nursing, the Association of Anaesthetists of Great Britain and Ireland and the Royal College of Anaesthetists. Excessive fasting could lead to discomfort and possible morbidity in surgical patients. The surgical team needs to collaborate to reduce the fasting time by revising the operative list.
Synthesis of Polymetallic Oxides with High Organized Structure
Authors: Dumitrescu Anca Mihaela , Jucan Victor , Iordan Alexandra Raluca , Palamaru Mircea Nicolae 
Published: July 2016

Abstract
The synthesis and characterization of high organized and nanostructured polymetallic oxides were studied. The samples were synthesized by sol-gel autocombustion method, using glycine as chelating/fuel agent and polyacrylamide-based hydrogel as template agent. This polymer has a porous microstructure with well-individualized and regularly-dispersed pores. The asobtained samples were characterized by IR spectroscopy, XRD, SEM. The magnetic properties were studied. As-synthesized polymetallic oxides have the morphological structure given by the three-dimensional lattice structure of hydrogel used as template agent
Portuguese older people and the Internet: Interaction, uses, motivations, and obstacles
Authors: Martinez-Pecino Roberto , Delerue Matos Alice , Silva Patricia 
Published: July 2016

AbstractThis study analyzes Portuguese seniors’ Internet activity and determine their reasons, benefits, and motivations for web use as well as the obstacles faced by non-users. Results were derived from a questionnaire completed by 189 seniors enrolled in universities for seniors. 68.1% defined themselves as Internet users. The seniors asked principally go online to check e-mail and gather information. They state that the Internet is useful, helps them to stay up-to-date, and to preserve (but not expand) relationships. Non-user status is not attributed to old age or health reasons. According to the technology acceptance model, ease of use and perception of utility were related to both Internet use and its intensity of use. Family influence also played a key role. Gender differences were not found in Internet use, intensity of use, or in use motivations.
Mediatization in public bureaucracies: A typology
Authors: Thorbjornsrud Kjersti , Ustad Figenschou Tine , Ihlen Øyvind 
Published: July 2016

AbstractBased on extensive fieldwork, the present article illustrates how the logic of the news media is expanding from influential communication departments to the practices, routines and priorities of traditional career bureaucrats. To theorize the mediatization of a traditional bureaucratic rationale, the article proposes a typology for how rule-based public organizations adapt to and adopt the news media’s implicit ‘logic of appropriateness.’ We emphasize the importance of (1) the news rhythm and (2) news formats, but also (3) how and why being in the media is valued by civil servants, and (4) how this leads to a reallocation of resources and responsibilities within the organization. We find that career bureaucrats both anticipate and adopt a news logic in their daily work. The normative implications of these transformations are discussed in the final section of the article.
Hodge correlators
Authors: Goncharov Alexander B. 
Published: July 2016

Abstract
Hodge correlators are
complex numbers given by certain integrals assigned to a smooth complex curve.
We show that they are correlators of a Feynman integral, and describe the real mixed Hodge structure
on the pronilpotent completion of the fundamental group of the curve. We introduce motivic correlators, which are elements of the motivic Lie algebra and whose periods are the Hodge correlators. They describe the motivic fundamental group of the curve.
We describe variations of real mixed Hodge structures on a variety by certain connections
on the product of the variety by twistor plane. We call them twistor connections.
In particular, we define the canonical period map on variations of real mixed
Hodge structures. We show that the obtained period functions satisfy a simple
Maurer–Cartan type non-linear differential equation.
Generalizing this, we suggest a DG-enhancement of the subcategory of Saito’s Hodge complexes with smooth cohomology. We show that when the curve varies,
the Hodge correlators are the coefficients of the twistor connection describing the corresponding variation of real MHS.
Examples of the Hodge correlators include classical and elliptic polylogarithms, and their generalizations.
The simplest Hodge correlators on the modular curves are the Rankin–Selberg integrals. Examples of the motivic
correlators include Beilinson’s elements in the motivic cohomology, e.g. the ones delivering
the Beilinson–Kato Euler system on modular curves.
Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet
Authors: Arzanlou Mahdi , Mousavi Somayeh , Bakhshi Mounes , Khakvar Reza , Bandehagh Ali 
Published: July 2016

AbstractIn the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.
Assessment of control strategies against Cydia pomonella (L.) in Morocco
Authors: Iraqui Salma El , Hmimina M’hamed 
Published: July 2016

Abstract
The codling moth, Cydia pomonella (L.), is the key pest of apple production worldwide. In Morocco, there is a sustainable presence of codling moth causing considerable damage in apple orchards despite frequent applications of broad spectrum insecticides. For 12 years, sexual trapping and chemical control were performed and the development of the codling moth population was analysed in an orchard which was in the region of Azrou. The efficacy of some insecticides (azinphos-methyl, chlorpyriphos-ethyl, diflubenzuron, thiacloprid, methoxyfenozide, spinosad, and deltamethrin) was also evaluated on neonate larvae and compared with a laboratory sensitive strain. This procedure was done to assess an eventual resistance in Moroccan populations. The action threshold was usually exceeded, leading to an intensive chemical control, with an average frequency of 9 to 13 days. The chemical control was done according to the action persistence time of the insecticides and the trap captures. However, those two parameters are compromised in Moroccan conditions because of the high summer temperatures which disrupt the action of insecticides and exacerbate populations. The pheromone traps may become ineffective and useless. Neonate larvae were resistant to five insecticides out of seven. Such results suggest the presence of a cross resistance in local strains. Overall, the insect resistance, the functioning of the sexual traps, and some insecticides properties (persistence action, pre-harvest interval) are the key factors that could explain the failure to control these moths under Moroccan conditions.
Sinn und Wohlergehen
Authors: Rüther Markus , Muders Sebastian 
Published: July 2016

Abstract
Das Thema des Beitrages bildet die normative Wertdimension des sinnvollen Lebens, die innerhalb der Theorie des guten Lebens lange vernachlässigt wurde. Untersucht wird dabei das Verhältnis der Sinndimension zur Dimension des Wohlergehens innerhalb des guten Lebens, die gegenwärtig im Zentrum der ethischen Theoriebildung steht. Im Rahmen eines komparativen Abgleichs wird dafür argumentiert, dass es den gängigen Theorien des menschlichen Wohls - hedonischen Theorien, Wunsch-Ziel- Theorien und Objektive-Liste-Theorien - nicht gelingt, die Dimension des sinnvollen Lebens über die jeweils von ihnen verfochtene Konzeption des Wohlergehens vollständig zu beschreiben. Ihr Verhältnis zum Sinn ist allerdings durchaus unterschiedlich: Während Glück und Wunschbefriedigung weder notwendig noch hinreichend sind, erweist sich die Selbstvervollkommnung wenigstens als ein möglicher Kandidat zum Führen eines sinnvollen Lebens.