bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transient telomerase inhibition alters cell cycle kinetics
Authors: Thompson Connor AH , Gu Alice , Yang Sunny , Mathew Veena , Fleisig Helen B , Wong Judy MY 
Published: July 2017

ABSTRACTTelomerase is the ribonucleoprotein reverse transcriptase that catalyzes the synthesis of telomeres at the ends of linear human chromosomes and contributes to proper telomere-loop (T-loop) formation. Formation of the T-loop, an obligate step before cell division can proceed, requires the generation of a 3’-overhang on the G-rich strand of telomeric DNA via telomerase or C-strand specific nucleases. Here, we discover telomerase activity is critical for efficient cell cycle progression using transient chemical inhibition by the telomerase inhibitor imetelstat. Telomerase inhibition caused changes in cell cycle kinetics and increased the proportion of cells in G2 phase, suggesting delayed clearance through this checkpoint. Investigating the possible contribution of unstructured telomere ends to these cell cycle distribution changes, we observed that imetelstat treatment induced γH2AX DNA damage foci in a subset of telomerase-positive cells but not telomerase-negative primary human fibroblasts. Chromatin-immunoprecipitation with γH2AX antibodies demonstrated imetelstat treatment-dependent enrichment of this DNA damage marker at telomeres. Notably, the effects of telomerase inhibition on cell cycle profile alterations were abrogated by pharmacological inhibition of the DNA-damage-repair transducer ATM.Additionally, imetelstat potentiation of etoposide, a DNA-damaging drug that acts preferentially during S/G2 phases of the cell cycle, also depended on functional ATM signaling. Our results suggest that telomerase inhibition delays the removal of ATM-dependent DNA damage signals from telomeres in telomerase-positive cancer cells. This demonstrates for the first time that telomerase activity directly facilitates the progression of the cell cycle through modulation of transient telomere dysfunction signals.
Double insertion of transposable elements provides a substrate for the evolution of satellite DNA
Authors: McGurk Michael P. , Barbash Daniel A. 
Published: July 2017

AbstractEukaryotic genomes are replete with repeated sequences, in the form of transposable elements (TEs) dispersed across the genome or as satellite arrays, large stretches of tandemly repeated sequence. Many satellites clearly originated as TEs, but it is unclear how mobile genetic parasites can transform into megabase-sized tandem arrays. Comprehensive population genomic sampling is needed to determine the frequency and generative mechanisms of tandem TEs, at all stages from their initial formation to their subsequent expansion and maintenance as satellites. The best available population resources, short-read DNA sequences, are often considered to be of limited utility for analyzing repetitive DNA due to the challenge of mapping individual repeats to unique genomic locations. Here we develop a new pipeline called ConTExt which demonstrates that paired-end Illumina data can be successfully leveraged to identify a wide range of structural variation within repetitive sequence, including tandem elements. Analyzing 85 genomes from five populations ofDrosophila melanogasterwe discover that TEs commonly form tandem dimers. Our results further suggest that insertion site preference is the major mechanism by which dimers arise and that, consequently, dimers form rapidly during periods of active transposition. This abundance of TE dimers has the potential to provide source material for future expansion into satellite arrays, and we discover one such copy number expansion of the DNA transposonHoboto ~16 tandem copies in a single line. The very process that defines TEs —transposition— thus regularly generates sequences from which new satellites can arise.
Genome-wide association mapping and genomic prediction unravels CBSD resistance in aManihot esculentabreeding population
Authors: Kayondo Siraj Ismail , Pino Del Carpio Dunia , Lozano Roberto , Ozimati Alfred , Wolfe Marnin , Baguma Yona , Gracen Vernon , Samuel Offei , Ferguson Morag , Kawuki Robert , Jannink Jean-Luc 
Published: July 2017

AbstractCassava(Manihot esculentaCrantz), a key carbohydrate dietary source for millions of people in Africa, faces severe yield loses due to two viral diseases: cassava brown streak disease (CBSD) and cassava mosaic disease (CMD). The completion of the cassava genome sequence and the whole genome marker profiling of clones from African breeding programs (www.nextgencassava.org) provides cassava breeders the opportunity to deploy additional breeding strategies and develop superior varieties with both farmer and industry preferred traits. Here the identification of genomic segments associated with resistance to CBSD foliar symptoms and root necrosis as measured in two breeding panels at different growth stages and locations is reported. Using genome-wide association mapping and genomic prediction models we describe the genetic architecture for CBSD severity and identify loci strongly associated on chromosomes 4 and 11. Moreover, the significantly associated region on chromosome 4 colocalises with aManihot glazioviiintrogression segment and the significant SNP markers on chromosome 11 are situated within a cluster of nucleotide-binding site leucine-rich repeat (NBS-LRR) genes previously described in cassava. Overall, predictive accuracy values found in this study varied between CBSD severity traits and across GS models with Random Forest and RKHS showing the highest predictive accuracies for foliar and root CBSD severity scores.
The plant circadian clock influences rhizosphere community structure and function
Authors: Hubbard Charley J. , Brock Marcus T. , van Diepen Linda T.A. , Maignien Loïs , Ewers Brent E. , Weinig Cynthia 
Published: July 2017

AbstractPlants alter chemical and physical properties of soil, and thereby influence rhizosphere microbial community structure. The structure of microbial communities may in turn affect plant performance. Yet, outside of simple systems with pairwise interacting partners, the plant genetic pathways that influence microbial community structure remain largely unknown, as are the performance feedbacks of microbial communities selected by the host plant genotype. We investigated the role of the plant circadian clock in shaping rhizosphere community structure and function. We performed 16S rRNA gene sequencing to characterize rhizosphere bacterial communities of Arabidopsis thaliana between day and night time points, and tested for differences in community structure between wild-type (Ws) vs. clock mutant (toc1-21, ztl-30) genotypes. We then characterized microbial community function, by growing wild-type plants in soils with an overstory history of Ws, toc1-21 or ztl-30 and measuring plant performance. We observed that rhizosphere community structure varied between day and night time points, and clock misfunction significantly altered rhizosphere communities. Finally, wild-type plants germinated earlier and were larger when inoculated with soils having an overstory history of wild-type in comparison to clock mutant genotypes. Our findings suggest the circadian clock of the plant host influences rhizosphere community structure and function.
Transfer Learning from Markov models leads to efficient sampling of related systems
Authors: Sultan Mohammad M. , Pande Vijay S. 
Published: July 2017

AbstractWe recently showed that the time-structure based independent component analysis method from Markov state model literature provided a set of variationally optimal slow collective variables for Metadynamics (tICA-Metadynamics). In this paper, we extend the methodology towards efficient sampling of related mutants by borrowing ideas from transfer learning methods in machine learning. Our method explicitly assumes that a similar set of slow modes and metastable states are found in both the wild type (base line) and its mutants. Under this assumption, we describe a few simple techniques using sequence mapping for transferring the slow modes and structural information contained in the wild type simulation to a mutant model for performing enhanced sampling. The resulting simulations can then be reweighted onto the full-phase space using Multi-state Bennett Acceptance Ratio, allowing for thermodynamic comparison against the wild type. We first benchmark our methodology by re-capturing alanine dipeptide dynamics across a range of different atomistic force fields, including the polarizable Amoeba force field, after learning a set of slow modes using Amber ff99sb-ILDN. We next extend the method by including structural data from the wild type simulation and apply the technique to recapturing the affects of the GTT mutation on the FIP35 WW domain.
Relationship between budget allocation and growth of resources at the university libraries of Northern India – a study
Authors: Singh Har , Mahajan Preeti 
Published: July 2017

Purpose
The purpose of the study is to find out the budgetary allocation for books and journals (Print and Electronic) and growth of resources during 2003-2004 to 2012-2013. The study also explored the significant/non-significant relationship for budget allocated for books and journals (Print and Electronic) as well as growth of resources across the libraries. This will help the librarians of such universities to build their collection accordingly.

Design/methodology/approach
The data were collected with the help of a structured questionnaire. Moreover, information was obtained from the primary library records such as annual reports, budget estimates, ledgers and also the staff involved in the acquisition section. The hypotheses were tested using chi-square test. The relationship between budgetary allocation and growth of resources amongst the five university libraries during 2003-2004 to 2012-2013 were analysed and discussed.

Findings
Maharishi Dayanand University library had the highest budget for purchase of books (Rs.125 lakh), whereas Panjab University library had the highest budget (Rs.208 lakh) for purchase of journals (Print and Electronic) during 2012-2013. Panjab University library allocated Rs.18 lakh for purchase of books and Rs.208 lakh for purchase of journals (Print and Electronic) during 2012-2013. Punjabi University library allocated 16 lakh for purchase of books and 45 lakh for purchase of journals (Print and Electronic) during 2012-2013. Guru Nanak Dev University library allocated Rs.16.80 lakh as the budget for purchase of books and Rs.8.30 lakh for purchase of journals (Print and Electronic) from 2007-2008 to 2012-2013. Kurukshetra University library allocated Rs.9 lakh for purchase of books during 2010-2011 and 2011-2012, whereas it had allocated Rs.79.20 lakh for purchase of journals (Print and Electronic) during 2008-2009. Maharishi Dayanand University library allocated Rs.125 lakh for purchase of books during 2011-2012 and 2012-2013, whereas it allocated Rs.125 lakh for purchase of journals (Print and Electronic) during 2012-2013.

Research limitations/implications
The study was limited to five universities of Northern India, which comprised Panjab University, Chandigarh (PU); Punjabi University, Patiala (PbiU); Guru Nanak Dev University, Amritsar (GNDU); Kurukshetra University, Kurukshetra (KU); and Maharishi Dayanand University, Rohtak (MDU). The data related to budgetary allocation for resources and growth and cumulative growth of books and journals (print and electronic) during 2003-2004 to 2012-2013 were collected and analysed.

Originality/value
This is the first of its kind of study in these universities, which provides an overview of how much budget was allocated for collection development in these university libraries. The relation between budget allocation and growth of resources were compared, and their significant/non-significant relationship in five university libraries in North India was found.
Self-initiated international career transition: a qualitative case study of Pakistani immigrants to Canada
Authors: Malik Amina Raza , Manroop Laxmikant , Singh Parbudyal 
Published: July 2017

PurposeThe purpose of this study is to examine the career transition experiences of three immigrants from Pakistan in Canadian organisations using Nicholson’s four-phase transition cycle.Design/methodology/approachA case study approach was used, and data were collected through three in-depth, semi-structured interviews to determine how immigrants experience career transitions in Canadian organisations.FindingsThe findings show that all three participants had almost the same level of expectations before coming to Canada; however, there were significant differences in their career transition experiences. These findings demonstrate that immigrants’ career experiences can be understood and examined through the lens of Nicholson’s transition framework; their expectations and experiences at one stage subsequently affected their experiences at later stages.Research limitations/implicationsA longitudinal research design would be an excellent approach to explore immigrants’ career transition over time.Practical implicationsOrganisations hiring skilled immigrants need to be more supportive of their efforts in reducing immigrants’ feeling of underemployment and discrimination.Originality/valueThe study contributes to the immigrant literature by presenting a new way of looking at immigrants’ experiences in Canadian organisations. It also contributes to the career literature by extending the application of Nicholson transition cycle to an underrepresented population (i.e. immigrants) in careers research.
Positioning health professional identity: on-campus training and work-based learning
Authors: Christensen Mette Krogh , Henriksen Jette , Thomsen Kristian Raun , Lund Ole , Mørcke Anne Mette 
Published: July 2017

PurposeDrawing on positioning theory, the purpose of this paper is to characterize the activities and positions of students and supervisors at workplaces and on-campus skills training sites across the higher health professional educations of medicine, sports science, and nursing. Furthermore, the study explored the impact of work-based learning (WBL) and skills training on students’ personal professional identity development.Design/methodology/approachA qualitative case study was conducted across six workplace sites and three on-campus skills training sites with 20 days of observation and 21 in-depth interviews. The data were inductively analyzed resulting in the identification of 12 characteristic narratives. This was followed by abductive analysis using Harré’s concept of positioning as the theoretical framework.FindingsAcross the three higher health professional educations, work-based and on-campus skills training sites were characterized by two learning spaces with distinct positions, rights, and duties. The WBL sites gave the students rich opportunities to position themselves, act independently, and behave as professionals seriously striving for mastery. On the on-campus sites, the students behaved less seriously, and were conscious of their rights to try out things, get support, and have fun.Research limitations/implicationsThe authors recommend that future studies explore aspects of professional identity formation due to its consequences for curriculum design, including the distribution of simulated spaces and professional spaces in students’ learning environments.Originality/valueThis study adds to the empirical evidence and conceptual frameworks of personal and shared professional identity development in the field of skills and WBL, and it underlines the ongoing value of Harré’s positioning theory in educational research.
A study on ultrasonic welding of nonwovens used for surgical gowns
Authors: Eryuruk Selin Hanife , Karagüzel Kayaoglu Burçak , Kalaoglu Fatma 
Published: July 2017

PurposeSurgical gowns should be designed and produced using special techniques to provide barrier properties against potential risks during surgery and healthcare procedures. Ultrasonic welding is one of these methods used to produce surgical gowns with determined barrier properties. The purpose of this paper is to analyse bond strength and permeability properties of ultrasonically welded nonwoven fabrics and compare them with traditional sewing techniques.Design/methodology/approachIn this study, ultrasonic welding of nonwovens was performed to demonstrate its use as an assembly method. Performance requirements in the design of surgical gowns were determined. Fabric strengths and bond strengths of ultrasonic-welded and traditionally sewn fabrics were analysed. The performance properties, i.e., bond strength, air and water resistance of the fabrics and the joints obtained by ultrasonic and classical sewing methods were studied.FindingsAs a result, it was found that ultrasonic welding technique is a suitable method for joining layers in surgical gown production bringing the advantages of high water resistance together with acceptable bond strength.Originality/valueThe current study focuses on the use of ultrasonic welding of nonwovens used for disposable protective surgical gowns. Ultrasound welding technique was presented as an alternative to classic assembly methods and ultrasonic welding technology was applied to different fabric combinations simulating different layers in different joining sections of a surgical gown.
Green start-up finance – where do particular challenges lie?
Authors: Bergset Linda 
Published: July 2017

PurposeThe purpose of this paper is to shed some new light on the entrepreneurial finance of green start-ups, for which there has been little quantitative empirical evidence thus far. It explores what challenges green start-ups might experience when it comes to financial access.Design/methodology/approachThe paper includes a survey of start-ups in Finland, Germany and Sweden and the separate evaluation of the “greenness” of participating companies’ product/service portfolios based on Eurostat’s Environmental Goods and Service Sector classification. A logistic regression is carried out for different company phases for two measures of challenges (“difficulty accessing finance” and “rejection by investor/funder”).FindingsGreen start-ups as an overall group cannot be said to have more challenges in access to finance. Particularly, a lack in business education and a high level of innovativeness, however, seem to be lead to more challenges for green start-ups in accessing finance compared to other start-ups.Research limitations/implicationsFurther research might seek to identify which exact characteristics of innovative green start-ups lead to challenges in financial access, i.e. is it individual factors such as high risk levels, high investment sums, long development periods or a low return prospect – or is it rather a combination? It might, furthermore, be rewarding to investigate whether “interventions” of business-related training might reduce challenges.Social implicationsSuggestions are made for improved policy support to sustainable entrepreneurship in the case of green start-ups.Originality/valueThis research paper provides quantitative empirical analysis in a new research area, which has previously been predominantly theory based with some anecdotal observations as well as some early qualitative research.
Robust visual tracking via randomly projected instance learning
Authors: Cheng Fei , Liu Kai , Gong Mao-Guo , Fu Kaiyuan , Xi Jiangbo 
Published: July 2017

Purpose
The purpose of this paper is to design a robust tracking algorithm which is suitable for the real-time requirement and solves the mistake labeling issue in the appearance model of trackers with the spare features.

Design/methodology/approach
This paper proposes a tracker to select the most discriminative randomly projected ferns and integrates a coarse-to-fine search strategy in this framework. First, the authors exploit multiple instance boosting learning to maximize the bag likelihood and select randomly projected fern from feature pool to degrade the effect of mistake labeling. Second, a coarse-to-fine search approach is first integrated into the framework of multiple instance learning (MIL) for less detections.

Findings
The quantitative and qualitative experiments demonstrate that the tracker has shown favorable performance in efficiency and effective among the competitors of tracking algorithms.

Originality/value
The proposed method selects the feature from the compressive domain by MIL AnyBoost and integrates the coarse-to-fine search strategy first to reduce the burden of detection. This paper designs a tracker with high speed and favorable results which is more suitable for real-time scene.
Development of an intelligent e-healthcare system for the domestic care industry
Authors: Wong Bennie , Ho G.T.S. , Tsui Eric 
Published: July 2017

Purpose
In view of the elderly caregiving service being in high demand nowadays, the purpose of this paper is to develop an intelligent e-healthcare system for the domestic care industry by using the Internet of Things (IoTs) and Fuzzy Association Rule Mining (FARM) approach.

Design/methodology/approach
The IoTs connected with the e-healthcare system collect real-time vital sign monitoring data for the e-healthcare system. The FARM approach helps to identify the hidden relationships between the data records in the e-healthcare system to support the elderly care management tasks.

Findings
To evaluate the proposed system and approach, a case study was carried out to identify the association between the specific collected demographic data, behavior data and the health measurements data in the e-healthcare system. It is found that the discovered rules are useful for the care management tasks in the elderly healthcare service.

Originality/value
Knowledge discovery in databases uses various data mining techniques and rule-based artificial intelligence algorithms. This paper demonstrates complete processes on how an e-healthcare system connected with IoTs can support the elderly care services via a data collection phase, data analysis phase and data reporting phase by using the FARM to evaluate the fuzzy sets of the data attributes. The caregivers can use the discovered rules for proactive decision support of healthcare services and to improve the overall service quality by enhancing the elderly healthcare service responsiveness.
Sensibility analysis of MCDA using prospective in Brazilian energy sector
Authors: Gomes Carlos Francisco Simões , Costa Helder Gomes , de Barros Alexandre P. 
Published: July 2017

Purpose
The purpose of this paper is to present a hybrid modelling that combines concepts and techniques for scenario building together with a Multi-criteria Decision Aid (MCDA) outranking approach. The paper presents a case to illustrate the proposed methodology.

Design/methodology/approach
The research method is a qualitative and quantitative mixture and it is presented as a study case. Bibliographic research is used to construct the theoretical framework. There are a number of studies that develop a sensibility analysis in MCDA modelling; however, none of them explore the robustness of the MCDA solution with use of scenarios variation.

Findings
The methodology allows the criteria that must be taken into account, according to the decision makers’ values and preferences. It is interesting to note that, depending on the scenario, different weights were applied for each criterion, and the performances of alternatives under each criterion has changed as well.

Practical implications
This need arises in decision problems that are susceptible to the influence of scenario variation.

Originality/value
This proposal was applied to a real case that has taken into account six alternatives, with a prospective analysis of three scenarios, evaluated by four criteria. The authors use prospective scenarios to choose the criterion weights and alternatives evaluation.