bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large vessel occlusion mediated fluid attenuated inversion recovery signal intensity ratio is associated with stroke within 4.5 h
Authors: Wang Yajing , Mao Qianqian , Jiang Liang , Peng Mingyang , Chen Yu-Chen , Zhang Hong , Wang Liwei , Yin Xindao 
Published: October 2024

IntroductionThe primary objective was to investigate the value of the fluid attenuated inversion recovery (FLAIR) signal intensity ratio (SIR) in identifying stroke within 4.5 h. The secondary objective was to ascertain whether large vessel occlusion (LVO) mediated the relationship between the SIR and stroke within 4.5 h.MethodsWe analyzed 633 acute stroke patients within 24 h of clear symptom onset. The SIR and DWI-FLAIR mismatch were evaluated. First, we determined whether demographic variables, vascular risk factors and LVO were related to stroke within 4.5 h with multivariate logistic regression analyses and stratified regression analysis. Next, we used mediation analysis to determine whether LVO explained the association between SIR and stroke within 4.5 h. Finally, we used receiver operating characteristic (ROC) analysis to assess the value of SIR, independent variable, and multiparameter models in identifying stroke within 4.5 h and compared with DWI-FLAIR mismatch.ResultsHyperlipemia, LVO and SIR were associated with stroke within 4.5 h. Mediation analysis revealed that LVO partially mediated the relationship between SIR and stroke within 4.5 h (p &lt; 0.001). The multiparameter model (hyperlipemia, LVO and SIR) showed significantly improved performance (AUC 0.869) in identifying stroke within 4.5 h over DWI-FLAIR mismatch (0.684), hyperlipemia (0.632), LVO (0.667) and SIR (0.773) models.ConclusionSIR is associated with stroke within 4.5 h, and LVO partially mediates this relationship. A multiparameter model combining hyperlipemia, LVO and SIR can more accurately identify stroke within 4.5 h than individual parameter models.
New model to predict survival in advanced pancreatic ductal adenocarcinoma patients by measuring GGT and LDH levels and monocyte count
Authors: del Campo-Pedrosa Rocío , Martín-Carnicero Alfonso , González-Marcos Ana , Martínez Alfredo 
Published: October 2024

IntroductionPancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a poor survival outcome. Predicting patient survival allows physicians to tailor treatments to specific individuals. Thus, a simple and cost-effective prognosis model is sorely needed.MethodsThis retrospective study assesses the prognostic value of blood biomarkers in advanced and metastatic PDAC patients (n=96) from Spain. Cut-off points for hematological parameters were calculated and correlated with overall survival (OS) using Kaplan-Meier, log-rank test, robust Cox proportional hazards and logistic regressions.ResultsIn univariate analysis, individuals with low levels of GGT, LDH, ALP, leukocyte-, neutrophil- and monocyte counts showed significantly longer survival than patients with higher levels. In multivariate analysis, lower levels of GGT (HR (95%CI), 2.734 (1.223-6.111); p=0.014), LDH (HR (95%CI), 1.876 (1.035-3.400); p=0.038) and monocyte count (HR (95%CI), 1.657 (1.095-2.506); p = 0.017) remained significantly beneficial. In consequence, we propose a prognostic model based on logistic regression (AUC=0.741) of these three biomarkers as a pioneer tool to estimate OS in PDAC.ConclusionThis study has demonstrated that the joint use of GGT (&lt;92.00), LDH (&lt;220.00) and monocyte count (&lt;800) are independent positive prognostic factors in PDAC that can predict one-year survival in a novel prognostic logistic model.
Molecular insights and inhibitory dynamics of flavonoids in targeting Pim-1 kinase for cancer therapy
Authors: Alhadrami Hani A. , Sayed Ahmed M. , Hassan Hossam M. , Alhadrami Albaraa H. , Rateb Mostafa E. 
Published: October 2024

Pim-1 kinase, a serine/threonine kinase, is often overexpressed in various cancers, contributing to disease progression and poor prognosis. In this study, we explored the potential of flavonoids as inhibitors of Pim-1 kinase using a combination of molecular docking and steered molecular dynamics (SMD) simulations. Our docking studies revealed two main binding orientations for the flavonoid molecules. The SMD simulations showed that the binding mode with higher pulling forces was linked to stronger inhibitory activity, with a strong positive correlation (R2 ≈ 0.92) between pulling forces and IC50 values. Quercetin stood out as the most potent inhibitor, showing a pulling force of about 820 pN and an IC_(5) 0 of less than 6 µM. Further dynamic simulations indicated that quercetin’s hydroxyl groups at the C3, C-5 and C-7 positions formed stable hydrogen bonds with key residues GLU-121, Leu-44 and Val-126, respectively enhancing its binding stability and effectiveness. Our results emphasized the critical role of the hydroxyl group at the C-3 position, which plays a pivotal function in effectively anchoring these molecules in the active site of Pim-1 kinase. Principal component analysis (PCA) of Pim-1 kinase’s conformational changes revealed that potent inhibitors like quercetin, galangin, and kaempferol significantly restricted the enzyme’s flexibility, suggesting potential inhibitory effect. These findings provide insights into the structural interactions between flavonoids and Pim-1 kinase, offering a foundation for future experimental investigations. However, further studies, including in vitro and in vivo validation, are necessary to assess the pharmacological relevance and specificity of flavonoids in cancer therapy.
EcoFAB 3.0: a sterile system for studying sorghum that replicates previous field and greenhouse observations
Authors: Gupta Kshitiz , Tian Yang , Eudes Aymerick , Scheller Henrik V. , Singh Anup K. , Adams Paul D. , Andeer Peter F. , Northen Trent R. 
Published: October 2024

IntroductionStudying plant-microbe interactions is one of the key elements in understanding the path to sustainable agricultural practices. These interactions play a crucial role in ensuring survival of healthy plants, soil and microbial communities. Many platforms have been developed over the years to isolate these highly complex interactions however, these are designed for small model plants. This creates a need for complementary devices for larger plants, such as sorghum.MethodsThis work introduces a novel platform, EcoFAB 3.0, which is designed to enable studying bioenergy plants such as sorghum for up to 4 weeks in a controlled sterile environment. Several other advantages of this platform such as dark root chambers and user-friendly assembly are also discussed in this work.Results and discussionEcoFAB 3.0 was found to replicate previous greenhouse and field observations when comparing an engineered sorghum line overproducing 4-hydroxybenzoic acid (4-HBA) and wildtype (variety BTx430). Consistent with greenhouse and field observations, it was found that the engineered line of sorghum grown in EcoFAB 3.0 had a higher 4-HBA content and a lower dry biomass.
Polysomnographic insights into the attention-deficit/hyperactivity disorder and obstructive sleep apnea connection in children
Authors: Lacki Olga , Slaven James , Rushton Jerry , Rao Harish , Thompson Rohan , Jalou Hasnaa , Bandyopadhyay Anuja 
Published: October 2024

IntroductionThere is a high prevalence of sleep disturbances and disorders such as obstructive sleep apnea (OSA) in children with attention-deficit hyperactivity disorder (ADHD), though this relationship remains poorly characterized by objective measures. Polysomnographic studies (PSGs) on sleep disruptions in ADHD have yielded inconsistent results. Few have studied polysomnograms in stimulant-medicated vs. non-medicated children with ADHD+/-OSA. This study aimed to elucidate pathognomonic polysomnographic sleep disturbances in children diagnosed with ADHD+/-OSA.MethodsMedical charts and polysomnograms were retrospectively reviewed for children ages 4-18 who underwent overnight polysomnography at a tertiary care center from 2019 to 2022. ADHD diagnosis was determined by ICD code F90, and OSA was defined by apnea-hypopnea indices (AHI) ≥5 events/hour. Four groups were evaluated: children without OSA or ADHD, children with OSA alone, children with ADHD alone, and children with ADHD+OSA. Statistical analyses identified significant differences among variables of interest.Results4,013 children met the study criteria. 2,372 children were without OSA and without ADHD (59.1%), 1,197 with OSA alone (29.8%), 333 with ADHD alone (8.3%), and 111 with ADHD and OSA (2.8%). Insomnia (ICD code G47.00) was prevalent in children with ADHD alone. However, they exhibited significantly better sleep efficiency (SE), than children with OSA alone, and SE did not significantly differ from the other groups. No significant difference in periodic limb movements (PLMs) was found across all groups. The above results held true even after correcting for stimulant prescription.ConclusionThe increased frequency of clinical insomnia diagnoses in children with ADHD is not associated with any traditional parameters on polysomnogram. Innovative subclinical polysomnographic biomarkers are needed to identify sleep characteristics unique to ADHD. In children with both ADHD and OSA, PSG results do not reveal any distinctive sleep disturbances which cannot be better explained by OSA alone. These findings underscore the importance of screening for OSA in patients with ADHD and clinically assessing for other sleep concerns.
Risk, Attributable Fraction and Attributable Number of Cause-Specific Heat-Related Emergency Hospital Admissions in Switzerland
Authors: Schulte Florian , Röösli Martin , Ragettli Martina S. 
Published: October 2024

ObjectivesWe assessed the relationship between heat and emergency hospital admissions (EHAs) in Switzerland using clinically relevant metrics.MethodsApplying distributed lag non-linear models, we investigated temperature-admission associations between May and September 1998–2019 for various disease groups, by age class and gender. We estimated the relative risk (RR) for moderate (29°C) and extreme (34°C) daily maximum temperatures relative to disease-specific optimum temperature, and calculated attributable fractions (AFs) for hot days and the following week. We also calculated the total number of heat-related EHAs.ResultsWe attributed 31,387 (95% confidence interval: 21,567–40,408) EHAs to above-optimal temperatures, 1.1% (0.7%–1.4%) of the total. Extreme temperatures increased the EHA risk for mental, infectious and neurological diseases. We observed particularly high AFs due to extreme heat for dehydration (85.9%, 95% CI: 82.4%–88.8%) and acute kidney injury (AKI, 56.1%, 95% CI: 45.3%–64.7%). While EHA risk generally increased with age, we also found high RRs for infectious diseases in children (0–15 years) and AKI in young adults (15–64 years).ConclusionHot weather increases the EHA risk in Switzerland. Therefore a comprehensive clinical and public health response is needed.
Influence of Pediococcus pentosaceus Starter Cultures on Biogenic Amine Content and Antioxidant Activity in African Sourdough Flatbread Fermentation
Authors: Hassan Alaa Ahmed Alsiddig , Jin Young Hun , Mah Jae-Hyung 
Published: October 2024

This study investigated the impact of Pediococcus pentosaceus strains not only on biogenic amine (BA) content, but also on antioxidant indices, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and total phenolic content, in kisra, an African sourdough flatbread. Among forty-six lactic acid bacteria (LAB) strains isolated from naturally fermented kisra sourdough, two strains (K-B21, K-B01) identified as P. pentosaceus, were selected due to their low BA-producing and high BA-degrading ability for kisra fermentation. Inoculation with P. pentosaceus K-B21 or P. pentosaceus K-B01 completely prevented the formation of tyramine and cadaverine during kisra fermentation. The levels of putrescine, histamine, spermine, and spermidine in kisra were reduced by about 90%, >31%, 55–61%, and 9–25%, respectively, by the two strains, compared to the control (natural fermentation). Additionally, DPPH scavenging activity was 83–84% in the control and inoculated groups of kisra. The total phenolic content was 1977.60 μg/g in the control and insignificantly lower in the inoculated groups (1850–1880 μg/g) than the control. These results suggest that P. pentosaceus K-B21 and K-B01 are promising candidates for use as sourdough starter cultures to produce kisra bread of higher quality, including both its safety and health functionality.
EMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the Drosophila Muscle
Authors: Couto-Lima Carlos Antonio , Machado Maiaro Cabral Rosa , Anhezini Lucas , Oliveira Marcos Túlio , Molina Roberto Augusto da Silva , da Silva Rodrigo Ribeiro , Lopes Gabriel Sarti , Trinca Vitor , Colón David Fernando , Peixoto Pablo M. , Monesi Nadia , Alberici Luciane Carla , Ramos Ricardo Guelerman P. , Espreafico Enilza Maria 
Published: October 2024

EMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER–mitochondria contact, and lipid exchange. Here, we show that the Drosophila melanogaster EMC1 gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific EMC1 RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an EMC1 transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with an altered SR network, cytosolic calcium overload, and mitochondrial dysfunction and dysmorphology that impaired membrane potential and oxidative phosphorylation capacity. Genes coding for ER stress sensors, mitochondrial biogenesis/dynamics, and other EMC components showed altered expression and were mostly rescued by the EMC1 transgene expression. In conclusion, EMC1 is required for the SR network’s mitochondrial integrity and influences underlying programs involved in the regulation of muscle mass and shape. We believe our data can contribute to the biology of human diseases caused by EMC1 mutations.
Full-Length Transcriptome Analysis and Characterization of DFRs Involved in the Formation of Anthocyanin in Allium wallichii
Authors: Ju Zhigang , Liang Lin , Shi Hongxi , Zheng Yaqiang , Zhao Wenxuan , Sun Wei , Pang Yuxin 
Published: October 2024

Allium wallichii is famous for its reddish-purple flowers, which can be utilized as cut flowers and garden landscaping. Flower color is mainly determined by flavonoids, betalains, carotenoids, as well as other pigments. However, there is no research on the color formation mechanism in A. wallichii, which restricts its genetic improvement and development of superior varieties. The flower of A. wallichii was collected for full-length transcriptome sequencing and metabolome analysis using PacBio SMART and UPLC-MS, respectively. A total of 45 anthocyanins were detected in its flower, and 75,778 transcripts of 107,208 non-redundant transcripts were annotated. Then, two AwDFRs were cloned and characterized using bioinformatics tools. Enzyme activity assays revealed that both AwDFR1 and AwDFR2 possessed DFR activity in vitro that only accepted DHQ and DHM as substrates, except for DHK. Finally, physiological results showed that AwDFR1 and AwDFR2 could restore the lacking phenotypes of Arabidopsis tt3 mutant and increase the content of anthoycanin in tobacco petals. The anthocyanins and transcriptome in A. wallichii were firstly reported, and AwDFR1 and AwDFR2 are key enzymes participating in the biosynthesis of anthocyanins. This research provides important guidance for future key gene mining, color improvement, and horticultural breeding in A. wallichii.
Area-Time-Efficient Secure Comb Scalar Multiplication Architecture Based on Recoding
Authors: Zhang Zhantao , Wang Weijiang , Zhang Jingqi , He Xiang , Ma Mingzhi , Ren Shiwei , Dang Hua 
Published: October 2024

With the development of mobile communication, digital signatures with low latency, low area, and high security are in increasing demand. Elliptic curve cryptography (ECC) is widely used because of its security and lightweight. Elliptic curve scalar multiplication (ECSM) is the basic arithmetic in ECC. Based on this background information, we propose our own research objectives. In this paper, a low-latency and low-area ECSM architecture based on the comb algorithm is proposed. The detailed methodology is as follows. The recoding-k algorithm and randomization-Z algorithm are used to improve security, which can resist sample power analysis (SPA) and differential power analysis (DPA). A low-area multi-functional architecture for comb is proposed, which takes into account different stages of the comb algorithm. Based on this, the data dependency is considered and the comb architecture is optimized to achieve a uniform and efficient execution pattern. The interleaved modular multiplication algorithm and modified binary inverse algorithm are used to achieve short clock cycle delay and high frequency while taking into account the need for a low area. The proposed architecture has been implemented on Xilinx Virtex-7 series FPGA to perform ECSM on 256-bits prime field GF(p). In the hardware architecture with only 7351 slices of resource usage, a single ECSM only takes 0.74 ms, resulting in an area-time product (ATP) of 5.41. The implementation results show that our design can compete with the existing state-of-the-art engineering in terms of performance and has higher security. Our design is suitable for computing scenarios where security and computing speed are required. The implementation of the overall architecture is of great significance and inspiration to the research community.
Evaluation of the impact of specific oral health parameters on the quality of life in children with rare disorders of phosphorus-calcium metabolism
Authors: Alekseeva I. A. , Kiselnikova L. P. 
Published: October 2024

Relevance. Understanding the impact of oral health problems on the quality of life in children with rare metabolic disorders that affect mineral metabolism is critically important.Materials and methods. An oral health assessment was conducted on 59 children aged 6 to 17 years with rare diseases affecting mineral metabolism, each with a genetically confirmed diagnosis. The children were divided into two study groups: the first group (24 children) comprised those with bone mineralization disorders (hypophosphatemic rickets, hypophosphatasia), while the second group (35 children) included those with disorders of bone and cartilage formation (osteogenesis imperfecta, Marfan syndrome, achondroplasia, etc.). The clinical condition of the children's dental tissues was assessed by evaluating oral hygiene levels and caries intensity in permanent teeth, using the OHI-S and DMFT indices, respectively. The pufa/PUFA index was used to document complications arising from dental caries. To assess the perception of oral health problems, a survey was administered to parents and legal guardians using the Oral Health-Related Quality of Life (OHRQoL) questionnaire.Results. The average DMFT and OHI-S index values did not show statistically significant differences, with values of 4.26 ± 0.28 and 1.96 ± 0.15 in the first group, and 3.76 ± 0.40 and 1.75 ± 0.10 in the second group, respectively. However, the mean pufa/PUFA index values were significantly higher in children with bone and cartilage formation disorders (p = 0.003), being three times greater than those in the group with bone mineralization disorders, with values of 1.03 ± 0.18 and 0.30 ± 0.11, respectively. This increase was associated with higher correlation coefficients between the DMFT and PUFA indices and the modules for physical discomfort, oral functional status, and emotional well-being, with values of 0.38 and 0.41, and 0.49 and 0.27, compared to 0.07 and 0.02, and 0.29 and -0.21 in the first group. These differences were statistically significant at p < 0.05 and p < 0.005.Conclusion. The study revealed that existing oral health conditions have the greatest impact on the quality of life in children with rare diseases affecting bone and cartilage formation.
Progression of TMJ disorders in patients with rheumatoid arthritis
Authors: Lazarev Yu. V. , Antonova I. N. , Lazareva S. E. , Petrova M. S. , Inamova O. V. 
Published: October 2024

Relevance. The literature highlights the significant impact of rheumatoid arthritis (RA) on the onset of musculoskeletal dysfunction symptoms in the temporomandibular joint (TMJ). However, the risk factors that influence the clinical manifestations of TMJ disorders in RA patients remain insufficiently explored.Objective: To examine the impact of rheumatoid arthritis on the onset and progression of TMJ disorders.Materials and methods. At the Dental Clinic of the Central Research Institute of Stomatology and Maxillofacial Surgery, 180 patients from St. Petersburg State Clinical Rheumatology Hospital No. 25, diagnosed with rheumatoid arthritis, were examined. Standard clinical assessments and detailed medical history collection were performed.Results. Patients with long-standing RA (over 5 years) were more likely to experience TMJ disorder symptoms, with a relative risk of 1.342 (95% CI) [1.106; 1.628]. No statistically significant correlation was found between RA activity and TMJ disorders. The symptom pattern varied by timing: prior to RA, patients predominantly experienced TMJ clicking and crepitus (66%); during RA onset, pain and restricted jaw movement were more common (80% and 72%, respectively). After RA manifestation, pain became the dominant symptom, though its intensity diminished over time (to 60%).Conclusions. The duration of RA has a notable impact on both the frequency and nature of TMJ disorder symptoms. While RA treatment reduces symptom intensity over time, their frequency remains high.
Identifikasi Miskonsepsi Pada Konsep Ion Menggunakan Tes Diagnostik Four-Tier Test Berbasis Representasi Jamak
Authors: Sukaria Muhammad Ikhsan 
Published: October 2024

Penelitian ini bertujuan untuk mengidentifikasi miskonsepsi yang dialami dengan menggunakan instrumen diagnostic four-tier. Metode penelitian yang digunakan adalah deskripstif kualitatif dengan menggambarkan beragam miskonsepsi yang dialami siswa pada konsep ion. Tes diagnostik four-tier digunakan untuk menemukan miskonsepsi ini. Tes diagnostik four-tier terdiri dari empat tingkat pertanyaan. Tingkat pertama mengacu pada pertanyaan utama, tingkat kedua mengacu pada keyakinan siswa dalam menjawab pertanyaan tingkat pertama, tingkat ketiga mengacu pada alasan siswa memilih pertanyaan tingkat pertama, dan tingkat keempat mengacu pada keyakinan siswa dalam menjawab pertanyaan tingkat ketiga. Representasi pada soal menggunakan representasi jamak yang terdiri dari representasi makroskopik, submikroskopik, dan representasi simbol. Instrument yang dikembangkan valid ditunjukkan dengan pengujian validitas oleh validator ahli. Subjek penelitian, siswa SMP kelas IX yang mempelajari materi atom molekul dan ion, memiliki reliabilitas tes sebesar 0,6. Penelitian ini berhasil menemukan miskonsepsi yang terjadi pada siswa yang berkaitan dengan konsep ion antara lain ; molekul merupakan gabungan ion-ion; muatan positif pada ion menandakan pertambahan jumlah elektron sedangkan muatan negatif menandakan kehilangan elektron; molekul dan ion merupakan bentuk lain dari atom.