bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modafinil and cognitive enhancement in schizophrenia and healthy volunteers: the effects of test battery in a randomised controlled trial
Authors: Lees J. , Michalopoulou P. G. , Lewis S. W. , Preston S. , Bamford C. , Collier T. , Kalpakidou A. , Wykes T. , Emsley R. , Pandina G. , Kapur S. , Drake R. J. 
Published: May 2017

BackgroundCognitive deficits in schizophrenia have major functional impacts. Modafinil is a cognitive enhancer whose effect in healthy volunteers is well-described, but whose effects on the cognitive deficits of schizophrenia appear to be inconsistent. Two possible reasons for this are that cognitive test batteries vary in their sensitivity, or that the phase of illness may be important, with patients early in their illness responding better.MethodsA double-blind, randomised, placebo-controlled single-dose crossover study of modafinil 200 mg examined this with two cognitive batteries [MATRICS Consensus Cognitive Battery (MCCB) and Cambridge Neuropsychological Test Automated Battery (CANTAB)] in 46 participants with under 3 years’ duration of DSM-IV schizophrenia, on stable antipsychotic medication. In parallel, the same design was used in 28 age-, sex-, and education-matched healthy volunteers. Uncorrectedpvalues were calculated using mixed effects models.ResultsIn patients, modafinil significantly improved CANTAB Paired Associate Learning, non-significantly improved efficiency and significantly slowed performance of the CANTAB Stockings of Cambridge spatial planning task. There was no significant effect on any MCCB domain. In healthy volunteers, modafinil significantly increased CANTAB Rapid Visual Processing, Intra-Extra Dimensional Set Shifting and verbal recall accuracy, and MCCB social cognition performance. The only significant differences between groups were in MCCB visual learning.ConclusionsAs in earlier chronic schizophrenia studies, modafinil failed to produce changes in cognition in early psychosis as measured by MCCB. CANTAB proved more sensitive to the effects of modafinil in participants with early schizophrenia and in healthy volunteers. This confirms the importance of selecting the appropriate test battery in treatment studies of cognition in schizophrenia.
Pathways from assaultive violence to post-traumatic stress, depression, and generalized anxiety symptoms through stressful life events: longitudinal mediation models
Authors: Lowe S. R. , Joshi S. , Galea S. , Aiello A. E. , Uddin M. , Koenen K. C. , Cerdá M. 
Published: May 2017

BackgroundAssaultive violence events are associated with increased risk for adverse psychiatric outcomes, including post-traumatic stress (PTS), depression, and generalized anxiety. Prior research has indicated that economic, legal, and social stressors that could follow assaultive events may explain the increased risk for adverse psychiatric outcomes, yet longitudinal studies have not adequately examined this pathway. In the current study, we aimed to address this limitation.MethodsParticipants (N = 1360) were part of a longitudinal population-based study of adults living in Detroit. At three waves, participants indicated their exposure to assaultive violence and economic, legal, and social stressors, and completed inventories of PTS, depression, and generalized anxiety. Longitudinal mediation models were used to test the hypothesized pathway from assaultive violence to each psychiatric outcome.ResultsThe hypothesized models evidenced good fit with the data and, in each, the paths from Wave 1 (W1) assaultive violence to W2 stressors, and from W2 stressors to W3 symptoms were significant (range of Standardized Estimates: 0.09–0.15, all p < 0.01). Additionally, the indirect paths from W1 assaultive violence to W3 symptoms were significant (range of Standardized Estimates: 0.01–0.02, all p < 0.05).ConclusionsThe findings illustrate that the economic, legal, and social stressors that could follow assaultive violence increase risk for a range of psychiatric symptoms. Although future research is needed, the results suggest that investment in interventions that prevent and mitigate assaultive violence survivors’ exposure to such stressors may be an effective way to prevent mental illness in the aftermath of violent assaults.
Social network cohesion and the retreat from Southern vowels in Raleigh
Authors: Dodsworth Robin , Benton Richard A. 
Published: May 2017

AbstractNetwork research in sociolinguistics suggests that integration in a local community network promotes speakers' retention of local linguistic variants in the context of pressure from external or standard dialects. In most sociolinguistic network research, a speaker is assigned a single score along an index representing the aggregate of several network and other social features. We propose that contemporary network methods in adjacent disciplines can profitably apply to sociolinguistics, thereby facilitating not only more generalizable quantitative analysis but also new questions about therelationalnature of linguistic variables. Two network analysis methods—cohesive blocking and Quadratic Assignment Procedure regression—are used to evaluate the social network factors shaping the retreat from the Southern Vowel Shift (SVS) in Raleigh, North Carolina. The data come from a 160-speaker subset of a conversational corpus. Significant network effects indicate that network proximity to Raleigh's urban core promotes retention of SVS features, and that network similarity between speakers corresponds to linguistic similarity. Contemporary social-network methods can contribute to linguistic analysis by providing a holistic picture of the community's structure. (Networks, sociophonetics, Southern Vowel Shift, dialect contact)*
Jaw setting and the California Vowel Shift in parodic performance
Authors: Pratt Teresa , D'Onofrio Annette 
Published: May 2017

AbstractThis article explores the intertwining semiotics of language and embodiment in performances of Californian personae. We analyze two actors’ performances of Californian characters in parodic skits, comparing them to the same actors’ performances of non-Californian characters. In portraying their Californian characters, the actors use particularized jaw settings, which we link toembodied stereotypesfrom earlier portrayals of the Valley Girl and Surfer Dude personae. Acoustic analysis demonstrates that both actors also produce features of the California Vowel Shift in their Californian performances, aligning their linguistic productions with sound changes documented in California. We argue that these embodied stereotypes and phonetic realizations not only co-occur in parodic styles, but are in fact semiotically and corporeally intertwined, one occasioning the other. Moreover, the performances participate in the broader process ofenregisterment, packaging these semiotic resources with other linguistic and extralinguistic features to recontextualize Californian personae in the present day. (Parody, performance, California, California Vowel Shift, embodiment, embodied stereotype, enregisterment)*
Policy convergence as a multifaceted concept: the case of renewable energy policies in the European Union
Authors: Strunz Sebastian , Gawel Erik , Lehmann Paul , Söderholm Patrik 
Published: May 2017

AbstractThe literature on policy convergence has identified numerous facets and causal drivers of convergence. Distinguishing four dimensions of convergence (object, benchmark, drivers and directed process) helps clarify why and in what form policy convergence may occur (or not). Thus, depending on, for example, the object of analysis (policy outcome or instruments used), the same empirical case may give rise to opposing assessments. Furthermore, both economic and political drivers are necessary to account for successful policy convergence: economic convergence partly explains why countries may face similar problems, and political mechanisms explain why they might choose similar policies to solve a given problem. This article illustrates the multifaceted character of convergence for the dynamic field of renewable energy policies in the European Union. The empirical results indicate temporary convergence in the case of policy support instrument choices and conditional convergence in terms of renewable shares. However, the results suggest divergence of public R&D subsidies targeting renewables.
How do family medicine residents choose an anticoagulation regimen for patients with nonvalvular atrial fibrillation?
Authors: Yazdan-Ashoori Payam , Oqab Zardasht , McIntyre William F. , Quinn Kieran L. , Oosten Erik van , Hopman Wilma M. , Baranchuk Adrian 
Published: May 2017

Aim
To examine the choices Canadian family medicine residents make for oral anticoagulation (OAC) for patients with nonvalvular atrial fibrillation (AF).

Background
AF increases the risk of strokes. An important consideration in AF management is risk stratification for stroke and prescription of appropriate OAC. Family physicians provide the vast majority of OAC prescriptions.

Methods
We administered a survey to residents in multiple Canadian family medicine training programmes. Questions explored the experiences and attitudes towards risk stratification and choices of OAC when presented with standardized clinical scenarios. In each scenario, a novel oral anticoagulant (NOAC) would be the preferred treatment according to the contemporary Canadian and European guidelines.

Findings
A total of 247 residents participated in the survey. Most used the congestive heart failure, hypertension, age ≥ 75, diabetes mellitus, stroke or TIA (2 points) (81%) and congestive heart failure, hypertension, age ≥ 75 (2 points) or age 65-74 (1 point), diabetes mellitus, stroke or TIA, vascular disease including peripheral arterial disease, myocardial infarction, or aortic plaque, sex (female) (67%) risk stratification schemes while the preferred bleeding risk stratification scheme was hypertension, abnormal liver or renal function, stroke, bleeding, labile international normalized ratio, elderly (age ≥ 65), drugs or alcohol (84%). In the clinical scenarios, residents generally preferred warfarin in favour of NOACs, independent of training level. Residents ranked the risk of adverse events and the cost to the patient as their most and least important consideration when prescribing OAC, respectively. Therefore in patients with nonvalvular AF, Canadian family medicine residents prefer warfarin in comparison with NOACs despite the latest Canadian and European guideline recommendations. This knowledge gap may be enhanced by multiple factors, including a sometimes magnified fear of adverse events and a rapidly changing landscape in stroke prophylaxis.
Evaluation of Central Asian wheat germplasm for stripe rust resistance
Authors: Kokhmetova Alma , Sharma Ram C. , Rsaliyev Shynbolat , Galymbek Kanat , Baymagambetova Kanagat , Ziyaev Zafar , Morgounov Alexey 
Published: May 2017

AbstractStripe rust, caused by Puccinia striiformis f.sp. tritici (Pst), is an important disease of winter wheat in Central Asia. Stripe rust races contain diverse virulence/avirulence patterns and change rapidly. Therefore the objectives of this research were to: (i) examine current pathotype variability of Pst races collected from Kazakhstan and Uzbekistan and (ii) evaluate stripe rust resistance in leading cultivars and advanced breeding lines targeted to those regions. Analyses of 152 Pst samples showed diverse virulence patterns with avirulence to Yr5, Yr10 and Yr15 being common. Most of identified races are among the rare. Analysis of a mixed Pst population showed 10 distinct pathotypes with frequencies ranged from 1.2 to 8.7%. The virulence patterns ranged from least ‘31–1.5’ and X-1.5 to highly virulent ‘86 + E16’. Seedling evaluation of 62 genotypes using the 10 pathotypes showed variations for resistance. Bunyodkor and Barhayot showed resistance to all pathotypes. Five Yr genes were postulated. Yr1 in KR12-5075, and Yr6 in KR11-03 and KR12-5003 were postulated. Yr5 combined with Yr10 and Yr15 genes were determined in Bunyodkor. The wheat genotypes also showed different levels of resistance in adult plant stage under field conditions. Twenty genotypes showed <20% severity in both Kazakhstan and Uzbekistan. The disease severity on several genotypes differed in this countries, suggesting different Pst populations in the two countries. Several resistant genotypes were identified, which should be further evaluated for release as new varieties or used in breeding programmes. Two resistant lines from this study were identified as new varieties in Georgia and Uzbekistan.
TCF1+ hepatitis C virus-specific CD8+ T cells are maintained after cessation of chronic antigen stimulation
Authors: Wieland Dominik , Kemming Janine , Schuch Anita , Emmerich Florian , Knolle Percy , Neumann-Haefelin Christoph , Held Werner , Zehn Dietmar , Hofmann Maike , Thimme Robert 
Published: May 2017

AbstractDifferentiation and fate of virus-specific CD8+ T cells after cessation of chronic antigen stimulation is unclear. Here we show that a TCF1+CD127+PD1+ hepatitis C virus (HCV)-specific CD8+ T-cell subset exists in chronically infected patients with phenotypic features of T-cell exhaustion and memory, both before and after treatment with direct acting antiviral (DAA) agents. This subset is maintained during, and for a long duration after, HCV elimination. After antigen re-challenge the less differentiated TCF1+CD127+PD1+ population expands, which is accompanied by emergence of terminally exhausted TCF1-CD127-PD1hi HCV-specific CD8+ T cells. These results suggest the TCF1+CD127+PD1+ HCV-specific CD8+ T-cell subset has memory-like characteristics, including antigen-independent survival and recall proliferation. We thus provide evidence for the establishment of memory-like virus-specific CD8+ T cells in a clinically relevant setting of chronic viral infection and we uncover their fate after cessation of chronic antigen stimulation, implicating a potential strategy for antiviral immunotherapy.
Transcriptional regulation of FOXP3 requires integrated activation of both promoter and CNS regions in tumor-induced CD8+ Treg cells
Authors: Chakraborty Sreeparna , Panda Abir K. , Bose Sayantan , Roy Dia , Kajal Kirti , Guha Deblina , Sa Gaurisankar 
Published: May 2017

AbstractT-regulatory cells are an upsurge in the tumor microenvironment and induce immune-evasion. CD4+ Treg cells are well characterized whereas the role of CD8+ Tregs in cancer has recently started to crease attention. Here, we report an augmentation CD8+FOXP3+ Tregs in breast tumor microenvironment. FOXP3, the lineage-specific transcription factor, is a dominant regulator of Treg cell development and function. FOXP3 is induced preferentially by divergent signaling in CD4+ Treg cells. But how FOXP3 is induced and maintained in tumor-CD8+ Tregs is the Cinderella of the investigation. We observed that RUNX3, a CD8+ lineage-specific transcription factor, binds at the FOXP3-promoter to induce its transcription. In addition to promoter activation, involvement of cis-elements CNS1 and CNS2 in the transcriptional regulation of FOXP3 was also evident in these cells. SMAD3 binds to CNS1 region and acts as transcription inducer, whereas GATA3 plays a temporal role in the FOXP3 transcription by differential chromatin modification in CNS regions. In CNS1 region, GATA3 acts as a repressor for FOXP3 in naïve CD8+ T cells. Whereas in CD8+ Tregs, GATA3 binds directly at CNS2 region and persuaded the maintenance of FOXP3. Therefore, the intervention of these concerted transcriptional machinery may have a therapeutic potential in immunotherapy of cancer.
Function of second cladding layer in hollow core tube lattice fibers
Authors: Huang Xiaosheng , Yoo Seongwoo , Yong KenTye 
Published: May 2017

AbstractModes attenuation of the tube lattice fiber (TLF) is characterized by D/λ, where D is the core diameter and λ is the wavelength. Hence, the TLF is structured with a large core to ensure a low attenuation loss. A small core, on the other hand, facilitates the gas-filled TLF applications, but at the expense of the increased mode attenuation. We show that adding a second cladding layer to the conventional one layer TLF (1TLF) can resolve the contradicting requirements. The mode attenuation of TLF with two cladding layers (2TLF) is less influenced by the D/λ value as compared to 1TLF, thus realizing a low loss small core TLF. Furthermore, we found that adding the second layer brings another advantage to a bending performance. With a determined core size, D, a 1TLF with smaller capillary hole size, d, experiences less bending loss. However, the reduced d increases the confinement loss that counteracts the bending loss improvement. This confliction is substantially alleviated in 2TLF thanks to the second cladding layer. Theoretical investigations and experimental demonstrations are presented to evidence the important role of the second cladding ring in the TLF, which has been overlooked in prior studies.
Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression
Authors: Liu Yang , Zhang Zhong , Liang Hui , Zhao Xuyang , Liang Ling , Wang Guangxi , Yang Jingyi , Jin Yan , McNutt Michael A. , Yin Yuxin 
Published: May 2017

AbstractEG5 (KIF11) is a member of the kinesin-like protein family involved in centrosome separation and bipolar spindle formation. When a cell enters mitosis, CDK1 phosphorylates EG5 at Thr926 and promotes EG5 localization on the mitotic spindle which drives bipolar spindle formation. EG5 provides power for spindle movement and thus controls the dynamics of spindle assembly. However, little is known about EG5 regulation or how EG5 detaches from the spindle upon mitotic exit. In this study we identify EG5 as a novel substrate of PP2A phosphatase, and we show that the PP2A/B55α complex plays an important role in mitotic exit by a mechanism involving EG5. The PP2A/B55α complex physically associates with the EG5 C-terminal tail domain and dephosphorylates EG5 at Thr926 that enables mitotic exit. Conversely PP2A knockdown cells show a high level of phospho-EG5 in late metaphase, which is associated with a delay in mitotic exit. These phenotypic features are similar to those induced by EG5/T926D transfection that mimics phosphorylated EG5 status. Our results argue that PP2A controls mitotic exit through EG5 dephosphorylation. Lack of PP2A leads to abnormal EG5 activation, resulting in delay of mitotic exit.
Stem cell proliferation patterns as an alternative for in vivo prediction and discrimination of carcinogenic compounds
Authors: Stevens An-Sofie , Willems Maxime , Plusquin Michelle , Ploem Jan-Pieter , Winckelmans Ellen , Artois Tom , Smeets Karen 
Published: May 2017

AbstractOne of the major challenges in the development of alternative carcinogenicity assays is the prediction of non-genotoxic carcinogens. The variety of non-genotoxic cancer pathways complicates the search for reliable parameters expressing their carcinogenicity. As non-genotoxic and genotoxic carcinogens have different cancer risks, the objective of this study was to develop a concept for an in vivo test, based on flatworm stem cell dynamics, to detect and classify carcinogenic compounds. Our methodology entails an exposure to carcinogenic compounds during the animal’s regeneration process, which revealed differences in proliferative responses between non-genotoxic and genotoxic carcinogens during the initial stages of the regeneration process. A proof of concept was obtained after an extensive study of proliferation dynamics of a genotoxic and a non-genotoxic compound. A pilot validation with a limited set of compounds showed that the proposed concept not only enabled a simple prediction of genotoxic and non-genotoxic carcinogens, but also had the power to discriminate between both. We further optimized this discrimination by combining stem cell proliferation responses with a phenotypic screening and by using specific knockdowns. In the future, more compounds will be tested to further validate and prove this concept.
Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily
Authors: Lanfranchi Elisa , Pavkov-Keller Tea , Koehler Eva-Maria , Diepold Matthias , Steiner Kerstin , Darnhofer Barbara , Hartler Jürgen , Van Den Bergh Tom , Joosten Henk-Jan , Gruber-Khadjawi Mandana , Thallinger Gerhard G. , Birner-Gruenberger Ruth , Gruber Karl , Winkler Margit , Glieder Anton 
Published: May 2017

AbstractHomology and similarity based approaches are most widely used for the identification of new enzymes for biocatalysis. However, they are not suitable to find truly novel scaffolds with a desired function and this averts options and diversity. Hydroxynitrile lyases (HNLs) are an example of non-homologous isofunctional enzymes for the synthesis of chiral cyanohydrins. Due to their convergent evolution, finding new representatives is challenging. Here we show the discovery of unique HNL enzymes from the fern Davallia tyermannii by coalescence of transcriptomics, proteomics and enzymatic screening. It is the first protein with a Bet v1-like protein fold exhibiting HNL activity, and has a new catalytic center, as shown by protein crystallography. Biochemical properties of D. tyermannii HNLs open perspectives for the development of a complementary class of biocatalysts for the stereoselective synthesis of cyanohydrins. This work shows that systematic integration of -omics data facilitates discovery of enzymes with unpredictable sequences and helps to extend our knowledge about enzyme diversity.
Increase the elongation at break of poly (lactic acid) composites for use in food packaging films
Authors: El-hadi Ahmed M. 
Published: May 2017

AbstractPoly (3-hydroxy butyrate) (PHB), cellulose nano crystal (CNC) and a plasticizer (TBC) are mixed together with PLLA with the aim to increase the elongation at break for use in the food packing sector. Spherical (CNC) and fibril nano crystal (CNF) were prepared by hydrolysis of microcrystalline cellulose (MCC) in distilled water, and then stirred using a magnetic stirrer for 15 days and ultrasonic treatment without using any acids as green method. The morphology, thermal, and mechanical properties were studied using POM, DSC, WAXD, SEM and tensile testing, respectively. DSC demonstrated that the addition of PHB, CNC and TBC to PLLA matrix lead to reduce Tg, TCCand Tmthan pure PLLA. FT-IR verified that the carbonyl group C=O appeared broad and some peaks in the PLLA composites 5, 6 and 7 shifted from 3.98 × 108to 4.07 × 108 Hz, at 3.54 × 108to 3.44 × 108 Hz, at 3.19 × 108to 3.13 × 108 Hz. Mechanical testing shows that pure PLLA is brittle, and the elongation at break of PLLA composites reaches up to 205%, making it suitable to use in food packaging.
Scoring Systems for Postthrombotic Syndrome
Authors: Ghanima Waleed , Sandset Per , Kahn Susan , Wik Hilde 
Published: May 2017

AbstractPostthrombotic syndrome (PTS) is the most common long-term complication after deep vein thrombosis (DVT) and is associated with reduced quality of life. There is no single objective test to diagnose the presence of PTS and it is usually diagnosed on the basis of typical symptoms and signs in a limb previously affected by DVT. Scoring systems for PTS are primarily developed as research tools, but could possibly also be useful in the clinical setting. A main advantage of a good scoring system is standardization of the diagnostic process. An optimal scoring system should be both sensitive and specific for PTS, but this has been difficult to achieve because the symptoms and signs of PTS can be similar to other conditions leading to complaints in the lower limb. In an effort to standardize the definition of PTS, in 2009, the International Society on Thrombosis and Haemostasis Subcommittee on Control of Anticoagulation reviewed available scales and recommended use of the Villalta scale as the most appropriate measure to diagnose and grade the severity of PTS. The aim of this article is to review the existing scoring systems for PTS and to present our view on the advantages and disadvantages of these diagnostic tools.