bluuebunny/crossref_metadata_2025_split huggingface dataset:
TERT upregulation promotes cell proliferation via degradation of p21 and increases carcinogenic potential
Authors: Mishima Masako , Takai Atsushi , Takeda Haruhiko , Iguchi Eriko , Nakano Shigeharu , Fujii Yosuke , Ueno Masayuki , Ito Takahiko , Teramura Mari , Eso Yuji , Shimizu Takahiro , Maruno Takahisa , Hidema Shizu , Nishimori Katsuhiko , Marusawa Hiroyuki , Hatano Etsuro , Seno Hiroshi 
Published: September 2024

AbstractTelomerase reverse transcriptase (TERT) gene aberration is detectable in >80% of cases with hepatocellular carcinoma (HCC). TERT reactivation is essential for cellular immortalization because it stabilizes telomere length, although the role of TERT in hepatocarcinogenesis remains unelucidated. To elucidate the significance of aberrant TERT expression in hepatocytes in inflammation‐associated hepatocarcinogenesis, we generated Alb‐Cre;TertTg mice, which overexpress TERT in the liver and examined their phenotype during chronic inflammation. Based on transcriptome data from the liver tissue of Alb‐Cre;TertTg mice, we examined the role of TERT in hepatocarcinogenesis in vitro. We also evaluated the relationship between TERT and cell‐cycle‐related molecules, including p21, in HCC samples. The liver tumor development rate was increased by TERT overexpression during chronic inflammation, especially in the absence of p53 function. Gene set enrichment analysis of liver tissues revealed that gene sets related to TNF‐NFκB signaling, cell cycle, and apoptosis were upregulated in Alb‐Cre;TertTg liver. A luciferase reporter assay and immunoprecipitation revealed that TERT interacted with NFκB p65 and enhanced NFκB promoter activity. On the other hand, TERT formed protein complexes with p21, cyclin A2, and cyclin E and promoted ubiquitin‐mediated degradation of p21, specifically in the G1 phase. In the clinical HCC samples, TERT was highly expressed but p21 was conversely downregulated, and TERT expression was associated with the upregulation of molecules related to the cell cycle. Taken together, the aberrant upregulation of TERT increased NFκB promoter activity and promoted cell cycle progression via p21 ubiquitination, leading to hepatocarcinogenesis. © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Beyond presence of symptoms: Self‐reported psychosocial distress interference among outpatient youth with cancer and other life‐limiting conditions
Authors: Gordon Mallorie L. , Bedoya Sima Z. , Fry Abigail , Casey Robert , Steele Amii , Ciampa Devon , Ruble Kathy , Pao Maryland , Wiener Lori 
Published: September 2024

AbstractBackgroundYouth with life‐limiting conditions face significant psychosocial challenges (e.g., symptoms of anxiety, depression, and pain) throughout illness and treatment. Without appropriate intervention, this can negatively affect long‐term outcomes (e.g., disease management, health‐related quality of life). Prompt identification and appropriate attention to distress can mitigate these effects. We aimed to determine the prevalence and severity of distress interference among outpatient youth with cancer and other life‐limiting conditions, using the Checking IN screener.ProcedureWithin a larger study across four hospital centers, English‐speaking pediatric outpatients aged 8–21, and a caregiver‐proxy‐reporter, completed a brief distress screener. Descriptive analyses were used to characterize the sample and evaluate reported distress symptoms.ResultsChecking IN was completed by 100 participants, aged 8–21 (M = 14.27, SD = 3.81); caregivers completed an equivalent proxy screener. Youth most frequently endorsed fatigue (moderate: n = 50, 50.0%; high: n = 21, 21.0%), paying attention (moderate: n = 45, 45.0%; high: n = 16, 16.0%), and sleep difficulty (moderate: n = 46, 46.0%; high: n = 13; 13.0%) as problematic. Caregivers proxy reported fatigue (moderate: n = 46, 46.0%; high: n = 32, 32.0%), worry (moderate: n = 56, 56.0%; high: n = 10, 10.0%), and sleep difficulty (moderate: n = 47, 47.0%; high: n = 14; 14.0%) as most problematic. Group differences between youth and caregiver responses were not significant.ConclusionsYouth self‐report via Checking IN can detect psychosocial distress interference. By directing resources based on real‐time assessment of symptom interference, there is potential to simplify outpatient psychosocial screening and improve referral timeliness and specificity, thus allowing for more effective attention to evolving symptoms of distress.
Reduced multiscale complexity of daily behavioral dynamics in autism spectrum disorder
Authors: Nakamura Toru , Sumiyoshi Tomiki , Kamio Yoko , Takahashi Hidetoshi 
Published: September 2024

AbstractAimAutism spectrum disorder (ASD) is difficult to diagnose objectively due to its heterogeneous and complex manifestations. This study aimed to objectively characterize the behavioral phenotypes of ASD children by exploring the multiscale behavioral dynamics.MethodsWe applied behavioral organization (BO) and multiscale sample entropy (MSE) analyses to physical activity data collected from ASD and typically developing children, using wearable monitors in their daily life. We also examined their correlation with auditory startle response measures and clinical questionnaires, including the Social Responsiveness Scale (SRS) and the Strengths and Difficulties Questionnaire (SDQ).ResultsA significant decrease in MSE at timescales longer than 6 min was observed in ASD children, suggesting decreased irregularity or unpredictability, potentially linked to repetitive behaviors or stereotyped patterns commonly observed in ASD. Additionally, an increase in MSE positively correlated with prepulse inhibition levels, indicating its relationship with sensorimotor gating. Moreover, the observed significant negative correlation with the total difficulty score of SDQ substantiates MSE's potential as an objective metric for assessing general mental health problems associated with ASD.ConclusionMultiscale analysis enhances the understanding of ASD's behavioral dynamics, providing valuable metrics for real‐world assessments.
The effect of perceived parental conflict on middle school students' social anxiety: The role of self‐compassion and shame
Authors: Huang Siyuan , Huang Xishan , Luo Ruonan , Suzuki Ikue , Zou Lin , Chen Miao 
Published: September 2024

AbstractThis study explores the relationship between perceived parental conflict, self‐compassion, shame, and social anxiety and its internal mechanisms through two studies. Study 1 took 612 middle school students (285 women, 327 men, M = 14.18 years, SD = 1.72) as participants, and the Children's Perception of Interparental Conflict Scale, Self‐compassion scale (SCS), shame scale (SS), and Social Anxiety Scale were used for testing. The results showed that perceived parental conflict was significantly correlated with self‐compassion, shame, and social anxiety. Perceived parental conflict affects middle school students' social anxiety through feelings of self‐compassion and shame. In study 2, 106 middle school students (76 women, 30 men, M = 15.47 years, SD = 0.62) were randomly assigned to the self‐compassion group and control group, and students were asked to complete different literacy tasks, and then fill out the SCS, SS and adolescent social anxiety scale in turn. The results found that in the context of parental conflict, inducing the self‐compassion of middle school students can reduce the level of shame and social anxiety. The introduction of the concept of self‐compassion into the field of middle school students' mental health reveals the function of self‐compassion. It expands the relevant research on the intermediary role of self‐compassion, and also provides innovative ideas for the future research of mental health education in primary and secondary schools.
Mobilization with movement is effective for improving ankle range of motion and walking ability in individuals after stroke: A systematic review with meta‐analysis
Authors: Nascimento Lucas R. , Boening Augusto , Ribeiro Isabella , dos Santos Maria Eduarda , Benevides Marcelo , Santuzzi Cíntia H. 
Published: September 2024

AbstractBackgroundMobilization with movement has been recommended to improve foot mobility in people with ankle impairments and could improve walking after stroke.ObjectiveTo examine the effects of the addition of mobilization with movement to commonly used exercises in physiotherapy for improving ankle range of motion and walking in people who have had a stroke.DesignSystematic review of randomized controlled trials.SettingsNot applicable.ParticipantsAmbulatory adults at any time after stroke.InterventionThe experimental intervention was exercises plus ankle mobilization with movement, in comparison with exercises alone.Main outcomeAnkle range of motion.MeasurementsWalking parameters (ie., walking speed, cadence, step length).ResultsSix trials, involving 160 participants, were included. The mean PEDro score of the trials was 6 (range 4 to 7). Mobilization with movement in addition to exercises improved range of motion by 4° (95% CI 2 to 6), walking speed by 0.08 m/s (95% CI 0.05 to 0.11), cadence by 9 steps/min (95% CI 7 to 12), and step length by 5 cm (95% CI 3 to 7) more than exercises alone. The quality of evidence was low for range of motion and moderate for walking outcomes.ConclusionThis systematic review provided evidence that the addition of mobilization with movement to commonly used exercises in neurological rehabilitation increases ankle dorsiflexion, and benefits are carried over to improving walking speed, cadence, and step length in moderately disabled individuals with chronic stroke.Review RegistrationPROSPERO (CRD42023405130).
Seed‐Mediated Synthesis of Gold Nanobipyramids at Low CTAB Concentration Using Binary Surfactants
Authors: Zhang Er‐Ji , Deng Tian‐Song , Chen Zhuo , Liu Li‐Yong , Wu Gui‐Lin , Cheng Yu‐Chun , Gao Jia‐Fei , Wang Kun‐Peng , Liu Jie 
Published: September 2024

AbstractThe conventional method for synthesizing gold nanobipyramids (AuNBPs) is well established. However, the cytotoxicity resulting from the conjugates between hexadecyltrimethylammonium bromide (CTAB) and gold limits their biomedical applications. This study introduces a dual surfactant approach with CTAB and sodium oleate (NaOL) in the seed‐mediated synthesis of the AuNBPs, which can significantly reduce the CTAB concentration to 10 mM. Additionally, optimal growth conditions are achieved by varying the volumes of seed solution, AgNO3, and HCl. Under low CTAB concentrations, AuNBPs with tunable sizes and localized surface plasmon resonances between 600 and 1200 nm are successfully synthesized, as characterized by UV–vis‐NIR spectroscopy, transmission electron microscopy (TEM), and finite‐difference time‐domain (FDTD) simulations. This work demonstrates a method for synthesizing AuNBPs at low CTAB concentrations using binary surfactants and provides a framework applicable to the synthesis of other metallic nanomaterials.
Rapid and equipment‐free identification of papaya mealybug Paracoccus marginatus based on RPA‐CRISPR/Cas12a
Authors: Chen Yan‐Ting , Shi Meng‐Zhu , Chen Yan , Zhao Jian‐Wei , Yang Xiu‐Juan , Fu Jian‐Wei , Desneux Nicolas , Li Jian‐Yu 
Published: September 2024

AbstractBACKGROUNDParacoccus marginatus has invaded many countries, spreading rapidly and causing significant economic losses to crops. Accurate detection during the monitoring process is critical to prevent its expansion into new areas, therefore it is necessary to develop efficient and reliable detection methods. Traditional detection methods are time‐consuming and instrument‐dependent owing to the morphological similarities and small sizes of P. marginatus and other mealybugs, therefore establishing an efficient, rapid, and sensitive method for field detection in resource‐limited settings is critical.RESULTSA sensitive and rapid detection system was developed to detect P. marginatus using recombinase polymerase amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a. The RPA‐CRISPR/Cas12a assay distinguished P. marginatus from 10 other mealybugs. The entire process can be completed in approximately an hour, and the identification results can be determined by the naked eye using lateral flow strips or a portable mini‐UV torch. A method was developed to extract DNA from P. marginatus within 5 min. This method was combined with the RPA‐CRISPR/Cas12a assay to achieve rapid and simple detection. In addition, two portable thermos cups with temperature displays were used to maintain the reagents and assay reactions in the field.CONCLUSIONThis assay represents the first application of portable and easily available items (mini‐UV torch and thermos cup) based on the combination of RPA and CRISPR/Cas12a for rapid pest detection. This method is rapid, highly specific, and instrument‐flexible, allowing for the early monitoring of P. marginatus in the field. This study provides guidance for the development of suitable management strategies. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Paenibacillus polymyxa J2‐4 induces cucumber to enrich rhizospheric Pseudomonas and contributes to Meloidogyne incognita management under field conditions
Authors: Shi Qianqian , Fu Qi , Zhang Jie , Hao Guangyang , Liang Chen , Duan Fangmeng , Ma Juan , Zhao Honghai , Song Wenwen 
Published: September 2024

AbstractBACKGROUNDRoot knot nematodes (RKNs) pose a great threat to agricultural production worldwide. The bacterial nematocides have received increasing attention due to their safe and efficient control against RKNs. Here, we investigated the biocontrol efficacy of Paenibacillus polymyxa J2‐4 against Meloidogyne incognita in the field and analyzed the rhizosphere microbiome of cucumber under nematode infection after application of the J2‐4 strain. Furthermore, a biomarker strain of Pseudomonas spp. was isolated from the J2‐4‐inoculated rhizosphere soil, and its nematocidal activity and growth‐promoting effect on host plants were determined. In addition, chemotaxis assay of P. fluroescens ZJ5 toward root exudates was carried out.RESULTSThe field experiment demonstrated that P. polymyxa J2‐4 could effectively suppressed gall formation in cucumber plants, with the galling index reduced by 67.63% in 2022 and 65.50% in 2023, respectively, compared with controls. Meanwhile, plant height and yield were significantly increased in J2‐4 treated plants compared with controls. Metagenomic analysis indicated that J2‐4 altered the rhizosphere microbial communities. The relative abundance of Pseudomonas spp. was notably enhanced in the J2‐4 group, which was consistent with Linear discriminant analysis Effect Size results that Pseudomonas was determined as one of the biomarkers in the J2‐4 group. Furthermore, the ZJ5 strain, one of the biomarker Pseudomonas strains, was isolated from the J2‐4‐inoculated rhizosphere soil and was identified as Pseudomonas fluorescens. In addition, P. fluorescens ZJ5 exhibited high nematicidal activity in vitro and in vivo, with 99.20% of the mortality rate of M. incognita at 24 h and 69.75% of gall index reduction. The biocontrol efficiency of the synthetic community of ZJ5 plus J2‐4 was superior to that of any other single bacteria against M. incognita. Additionally, ZJ5 exhibited great chemotaxis ability toward root exudates inoculated with J2‐4.CONCLUSIONPaenibacillus polymyxa J2‐4 has good potential in the biological control against M. incognita under field conditions. Enrichment of the beneficial bacteria Pseudomonas fluorescens ZJ5 in the J2‐4‐inoculated rhizosphere soil contributes to M. incognita management. © 2024 Society of Chemical Industry.
Identification and functional characterization of the chitinase and chitinase‐like gene family in Myzus persicae (Sulzer) during molting
Authors: Zhang Chao , He Li , Ding Bo , Yang Hong 
Published: September 2024

AbstractBACKGROUNDThe crucial role of insect chitinase in molting, pupation, and emergence renders it a promising target for pest control strategies. Despite the extensive investigation of chitinase genes in various pests, there is still a lack of systematic identification and functional analysis related to aphid chitinase.RESULTSWe systematically identified a total of nine chitinase/chitinase‐like genes and one ENGase gene, which included eight Cht genes, one IDGF gene, and one ENGase gene. Through phylogenetic analysis, the chitinase proteins were classified into nine distinct groups (I, II, III, V, VI, VIII, X, other, and ENGase). The expression profile revealed that the epidermis exhibited relatively high expression levels for three chitinase genes: MpCht5, MpCht7, and MpCht10. Furthermore, transcriptional levels of nine chitinase genes were up‐regulated following treatment with 20‐hydroxyecdysone (20E) hormone. Silencing MpCht3, MpCht5, MpCht7, MpCht10, and MpCht11‐2 via RNA interference (RNAi) during the molting stage resulted in nymph shrinking, hindering normal molting and leading to death. Additionally, it was observed that silencing of MpIDGF induced the body color of the aphids to change from reddish brown to colorless after molting, culminating in eventual mortality.CONCLUSIONOur findings suggest that chitinase/chitinase‐like genes play a crucial role in the molting process of Myzus persicae. Utilizing RNAi technology, we aimed to elucidate the precise function of MpCht genes in the molting mechanism of M. persicae, this discovery establishes a significant theoretical foundation for future research on aphid control, with chitinase as the target. © 2024 Society of Chemical Industry.
The association between translocal mobility and well‐being of the “drifting elderly” in China
Authors: Chang Enyu , Zhang Min , Chen Peipei , Hu Yuchen 
Published: September 2024

AbstractMobility has been identified as having a key role in contributing to the well‐being of older adults. While existing studies have focused on the daily mobility of elderly people in a local context, relatively limited attention has been given to the translocal context of mobility of elderly migrants, who are increasingly involved in translocal mobility both physically and virtually. To fill this gap, focusing on the drifting elderly in China, this article examines the association between mobility and well‐being by integrating both local and translocal contexts. We deconstruct mobility into three forms: daily mobility within the host city, return mobility from the host city to the hometown, and virtual mobility which unifies local and translocal contexts. How these three forms of mobility relate to well‐being and the mediating role of both local and hometown social networks are investigated using structural equation models. The results indicate that daily mobility indirectly contributes to well‐being through the mediation of local social networks in the host city. Meanwhile, return mobility and virtual mobility have both direct and indirect positive effects on well‐being. Return mobility impacts well‐being mediated by hometown social networks, and virtual mobility contributes to well‐being through mediation by both daily mobility and hometown social networks. This article introduces a comprehensive translocal perspective, both physically and virtually, to the existing body of research, offering valuable insights into the intricate relationship between mobility and well‐being among elderly migrants.
The experiences of older migrant mothers and grandmothers with intergenerational solidarity in their transnational families
Authors: Pisaltu Ioana 
Published: September 2024

AbstractThe role of grandparents in providing support to their migrant adult children is well researched within the intergenerational cross‐border solidarity literature. Studies highlight the gendered care roles assumed by ‘left behind’ grandparents, usually women, who provide hands‐on caregiving to their young grandchildren. However, there is limited research on the supportive economic roles of older migrant women who help ‘left behind’ families navigate economically unpredictable circumstances through remittance transfers. This article makes a contribution by exploring the economic roles of older mothers and grandmothers who participate in the international labour market as paid care workers to support their families ‘back home.’ It draws on ethnographic research with Romanian women in their late 50s and 60s in Italy. Insight is gained into the experiences with transnational care and mobility of older mothers and grandmothers. More specifically, how expectations and demands for intergenerational solidarity in their families conditions women's migration projects, including its temporalities and the distribution of economic risks and responsibilities within the transnational family.
Spatial dynamics of incoming movers and the state‐led gentrification process: The case of Rotterdam
Authors: Janssen Kyri Maaike Joey , Cottineau‐Mugadza Clémentine , Kleinhans Reinout , van Bueren Ellen 
Published: September 2024

AbstractAlthough gentrification and its associated changes in residential mobility have been widely studied, surprisingly little attention has been paid to the changing origin locations of gentrification‐related residential moves. In this study, we use fine‐grained register data from the Dutch Central Bureau of Statistics to uncover changing residential mobility patterns to and within the city of Rotterdam, the Netherlands. We identify that the state‐led gentrification process goes hand in hand with the changing socioeconomic characteristics of in‐movers and the changing origin locations of residential moves. The city of Rotterdam increasingly attracts middle‐ to high‐income households from other core cities in the Netherlands, a process that we understand as inter‐urban gentrification spillover. In parallel, intra‐urban moves by economically vulnerable residents are declining, especially toward and within gentrifying neighborhoods. This represents evidence of exclusionary displacement. We conclude that the spillover effects of contemporary gentrification should be understood beyond an intra‐urban metropolitan perspective since gentrification in one city can enhance gentrification in another.
Quality by Design for Preclinical In Vitro Assay Development
Authors: Jones Jonathan , Zhang Bairu , Zhang Xiang , Konings Peter , Hansson Pia , Backmark Anna , Serrano Alessia , Künzel Ulrike , Novick Steven 
Published: September 2024

ABSTRACTQuality by Design (QbD) is an approach to assay development to determine the design space, which is the range of assay variable settings that should result in satisfactory assay quality. Typically, QbD is applied in manufacturing, but it works just as well in the preclinical space. Through three examples, we illustrate the QbD approach with experimental design and associated data analysis to determine the design space for preclinical assays.
The exponentially weighted control charts for geometrically moving standard deviations
Authors: Maghsoodloo Saeed , Silva Daniel F. 
Published: September 2024

AbstractThis article develops the control limits of Exponentially Weighted Moving Standard Deviation for Monitoring Gradual Shifts in Process variability, where the sample size n ≥ 2 stays constant in all subgroups. We have developed the Geometrically Moving control limits for the simple case of process standard deviation specified at σ0, and then for the case when σ is estimated from a subgroup of size m. We then obtained the conditional average run length by approximating the power function for testing the null hypothesis H0:σ = σ0 at the common sample sizes n = 4, 5, 6, 7, 8, 9, 10, 11 and 12. The results of this article are valid only if the normality assumption of underlying distribution is not violated.