bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suicide ideation among high-risk adolescent females: Examining the interplay between parasympathetic regulation and friendship support
Authors: Giletta Matteo , Hastings Paul D. , Rudolph Karen D. , Bauer Daniel J. , Nock Matthew K. , Prinstein Mitchell J. 
Published: December 2016

AbstractPoor physiological self-regulation has been proposed as a potential biological vulnerability for adolescent suicidality. This study tested this hypothesis by examining the effect of parasympathetic stress responses on future suicide ideation. In addition, drawing from multilevel developmental psychopathology theories, the interplay between parasympathetic regulation and friendship support, conceptualized as an external source of regulation, was examined. At baseline, 132 adolescent females (Mage = 14.59,SD= 1.39) with a history of mental health concerns participated in an in vivo interpersonal stressor (a laboratory speech task) and completed self-report measures of depressive symptoms and perceived support within a close same-age female friendship. Respiratory sinus arrhythmia (RSA) was measured before and during the speech task. Suicide ideation was assessed at baseline and at 3, 6, and 9 months follow-up. The results revealed that females with greater relative RSA decreases to the laboratory stressor were at higher risk for reporting suicide ideation over the subsequent 9 months. Moreover, parasympathetic responses moderated the effect of friendship support on suicide ideation; among females with mild changes or higher relative increases in RSA, but not more pronounced RSA decreases, friendship support reduced risk for future suicide ideation. Findings highlight the crucial role of physiological and external regulation sources as protective factors for youth suicidality.
Do dormancy-breaking temperature thresholds change as seeds age in the soil seed bank?
Authors: Liyanage Ganesha S. , Ooi Mark K.J. 
Published: December 2016

AbstractIn fire-prone ecosystems, many species regenerate after fire from persistent soil seed banks. Species with physically dormant (PY) seeds have dormancy broken by fire-related heat. The magnitude of post-fire recruitment, to predict response to varying fire severity, is commonly estimated by testing dormancy-breaking temperature thresholds of fresh PY seeds. However, seeds spend years in the soil during the inter-fire period, and determining whether dormancy-breaking thresholds change over time is essential to accurately predict population persistence. Germination of four south-eastern Australian PY species from the Fabaceae family (Acacia linifolia, Aotus ericoides, Bossiaea heterophylla and Viminaria juncea) were studied. Dormancy-breaking temperature thresholds vary inter-specifically and the species represented either high or low dormancy-breaking threshold classes. Freshly collected seeds, and seeds that had been buried in the field or stored in dry laboratory conditions for 6 and 18 months were subjected to a fire-related range of heat treatments (40–100°C). Seed ageing increased germination response to heat treatments, effectively lowering the dormancy-breaking thresholds of three species. The fourth species, A. linifolia, initially had a relatively large non-dormant fraction which was lost as seeds aged, with older seeds then displaying PY broadly similar to the other study species. Patterns of threshold decay were species-specific, with the thresholds and viability of low-threshold species declining more rapidly than high-threshold species. The non-dormant fraction did not increase over time for any of our study species. Instead of increasing their non-dormant fraction, as is common in other vegetation types, these fire-prone PY species displayed a change of dormancy-breaking temperature thresholds. This is an important distinction, as maintaining dormancy during the inter-fire period is essential for population persistence. While changes in sensitivity to dormancy-breaking treatments have previously been reported as seeds age, our study provides the first test of changes to temperature thresholds, which increases the range of germination response from the seed bank under varying fire severity.
Religion, Gendered Authority, and Identity in American Politics
Authors: Cassese Erin C. , Holman Mirya R. 
Published: December 2016

AbstractReligious identity serves as a central cleavage in American politics. However, little attention has been granted to how gendered views of authority conveyed in religious doctrine shape political identities and attitudes. Using a nation-wide sample of adult Americans, we demonstrate that gendered notions of divine and human authority exert considerable influence on political thinking. In particular, belief in a masculine God and preferences for traditional gender roles strongly relate to political conservatism. Adherence to gendered notions of authority influences political identity and policy preferences, even when controlling for more conventional indicators of religiosity. Accounting for gendered beliefs about authority also partially explains well-documented gender gaps in American politics, providing insight into women's apparently contradictory tendencies toward both political liberalism and religiosity. The relationships uncovered here, coupled with the continued salience of both gender and religion in contemporary political campaigns, underscore the importance of attending to the gendered dimensions of authority.
The ASEAN Economic Community: Deep Integration or Just Political Window Dressing?
Authors: Dosch Jörn 
Published: December 2016

AbstractOn 31 December 2015, the Association of Southeast Asian Nations (ASEAN) announced the establishment of the ASEAN Economic Community (AEC). In theory, this agreement has produced association-wide economic integration. However, following the announcement and for the foreseeable future, ASEAN member states will continue to have significantly less than full regional economic integration. Why? Some observers believe that the AEC plans involve an overly ambitious timeline and too many ill-thought-out initiatives. Others point to ASEAN's traditional aversion to legally binding agreements. While progress has been made in reducing or eliminating intra-ASEAN trade tariffs, substantial non-tariff barriers to trade persist. However, for most member states, the ASEAN market is relatively small, while external markets, especially China, are growing rapidly. Given this outward orientation for ASEAN trade, is the lack of an unhindered regional market really a problem?
Cullin-4 regulates Wingless and JNK signaling-mediated cell death in the Drosophila eye
Authors: Tare Meghana , Sarkar Ankita , Bedi Shimpi , Kango-Singh Madhuri , Singh Amit 
Published: December 2016

AbstractIn all multicellular organisms, the fundamental processes of cell proliferation and cell death are crucial for growth regulation during organogenesis. Strict regulation of cell death is important to maintain tissue homeostasis by affecting processes like regulation of cell number, and elimination of unwanted/unfit cells. The developing Drosophila eye is a versatile model to study patterning and growth, where complex signaling pathways regulate growth and cell survival. However, the molecular mechanisms underlying regulation of these processes is not fully understood. In a gain-of-function screen, we found that misexpression of cullin-4 (cul-4), an ubiquitin ligase, can rescue reduced eye mutant phenotypes. Previously, cul-4 has been shown to regulate chromatin remodeling, cell cycle and cell division. Genetic characterization of cul-4 in the developing eye revealed that loss-of-function of cul-4 exhibits a reduced eye phenotype. Analysis of twin-spots showed that in comparison with their wild-type counterparts, the cul-4 loss-of-function clones fail to survive. Here we show that cul-4 clones are eliminated by induction of cell death due to activation of caspases. Aberrant activation of signaling pathways is known to trigger cell death in the developing eye. We found that Wingless (Wg) and c-Jun-amino-terminal-(NH2)-Kinase (JNK) signaling are ectopically induced in cul-4 mutant clones, and these signals co-localize with the dying cells. Modulating levels of Wg and JNK signaling by using agonists and antagonists of these pathways demonstrated that activation of Wg and JNK signaling enhances cul-4 mutant phenotype, whereas downregulation of Wg and JNK signaling rescues the cul-4 mutant phenotypes of reduced eye. Here we present evidences to demonstrate that cul-4 is involved in restricting Wg signaling and downregulation of JNK signaling-mediated cell death during early eye development. Overall, our studies provide insights into a novel role of cul-4 in promoting cell survival in the developing Drosophila eye.
Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
Authors: Mikuła-Pietrasik Justyna , Uruski Paweł , Sosińska Patrycja , Maksin Konstantin , Piotrowska-Kempisty Hanna , Kucińska Małgorzata , Murias Marek , Szubert Sebastian , Woźniak Aldona , Szpurek Dariusz , Sajdak Stefan , Piwocka Katarzyna , Tykarski Andrzej , Książek Krzysztof 
Published: December 2016

AbstractAlthough both incidence and aggressiveness of ovarian malignancy rise with age, the exact reason for this tendency, in particular the contribution of senescent cells, remains elusive. In this project we found that the patient’s age determines the frequency of intraperitoneal metastases of ovarian cancer. Moreover, we documented that senescent human peritoneal mesothelial cells (HPMCs) stimulate proliferation, migration and invasion of ovarian cancer cells in vitro, and that this effect is related to both the activity of soluble agents released to the environment by these cells and direct cell-cell contact. The panel of mediators of the pro-cancerous activity of senescent HPMCs appeared to be cancer cell line-specific. The growth of tumors in a mouse peritoneal cavity was intensified when the cancer cells were co-injected together with senescent HPMCs. This effect was reversible when the senescence of HPMCs was slowed down by the neutralization of p38 MAPK. The analysis of lesions excised from the peritoneum of patients with ovarian cancer showed the abundance of senescent HPMCs in close proximity to the cancerous tissue. Collectively, our findings indicate that senescent HPMCs which accumulate in the peritoneum in vivo may create a metastatic niche facilitating intraperitoneal expansion of ovarian malignancy.
Differential regulated microRNA by wild type and mutant p53 in induced pluripotent stem cells
Authors: Grespi Francesca , Landré Vivien , Molchadsky Alina , Di Daniele Nicola , Marsella Luigi Tonino , Melino Gerry , Rotter Varda 
Published: December 2016

AbstractThe tumour suppressor p53 plays an important role in somatic cell reprogramming. While wild-type p53 reduces reprogramming efficiency, mutant p53 exerts a gain of function activity that leads to increased reprogramming efficiency. Furthermore, induced pluripotent stem cells expressing mutant p53 lose their pluripotency in vivo and form malignant tumours when injected in mice. It is therefore of great interest to identify targets of p53 (wild type and mutant) that are responsible for this phenotype during reprogramming, as these could be exploited for therapeutic use, that is, formation of induced pluripotent stem cells with high reprogramming efficiency, but no oncogenic potential. Here we studied the transcriptional changes of microRNA in a series of mouse embryonic fibroblasts that have undergone transition to induced pluripotent stem cells with wild type, knock out or mutant p53 status in order to identify microRNAs whose expression during reprogramming is dependent on p53. We identified a number of microRNAs, with known functions in differentiation and carcinogenesis, the expression of which was dependent on the p53 status of the cells. Furthermore, we detected several uncharacterised microRNAs that were regulated differentially in the different p53 backgrounds, suggesting a novel role of these microRNAs in reprogramming and pluripotency.
Short-term inhibition of TERT induces telomere length-independent cell cycle arrest and apoptotic response in EBV-immortalized and transformed B cells
Authors: Celeghin Andrea , Giunco Silvia , Freguja Riccardo , Zangrossi Manuela , Nalio Silvia , Dolcetti Riccardo , De Rossi Anita 
Published: December 2016

AbstractBesides its canonical role in stabilizing telomeres, telomerase reverse transcriptase (TERT) may promote tumorigenesis through extra-telomeric functions. The possible therapeutic effects of BIBR1532 (BIBR), a powerful TERT inhibitor, have been evaluated in different cellular backgrounds, but no data are currently available regarding Epstein–Barr virus (EBV)-driven B-cell malignancies. Our aim was to characterize the biological effects of TERT inhibition by BIBR on EBV-immortalized lymphoblastoid cell lines (LCLs) and fully transformed Burkitt’s lymphoma (BL) cell lines. We found that BIBR selectively inhibits telomerase activity in TERT-positive 4134/Late and 4134/TERT+ LCLs and EBV-negative BL41 and EBV-positive BL41/B95.8 BL cell lines. TERT inhibition led to decreased cell proliferation, accumulation of cells in the S-phase and ultimately to increased apoptosis, compared with mock-treated control cells. All these effects occurred within 72 h and were not observed in BIBR-treated TERT-negative 4134/TERT- and U2OS cells. The cell cycle arrest and apoptosis, consequent upon short-term TERT inhibition, were associated with and likely dependent on the activation of the DNA damage response (DDR), highlighted by the increased levels of γH2AX and activation of ATM and ATR pathways. Analyses of the mean and range of telomere lengths and telomere dysfunction-induced foci indicated that DDR after short-term TERT inhibition was not related to telomere dysfunction, thus suggesting that TERT, besides stabilizing telomere, may protect DNA via telomere-independent mechanisms. Notably, TERT-positive LCLs treated with BIBR in combination with fludarabine or cyclophosphamide showed a significant increase in the number of apoptotic cells with respect to those treated with chemotherapeutic agents alone. In conclusion, TERT inhibition impairs cell cycle progression and enhances the pro-apoptotic effects of chemotherapeutic agents in TERT-positive cells. These results support new therapeutic applications of TERT inhibitors in EBV-driven B-cell malignancies.
Functional roles of sialylation in breast cancer progression through miR-26a/26b targeting ST8SIA4
Authors: Ma Xiaolu , Dong Weijie , Su Zhen , Zhao Lifen , Miao Yuan , Li Nana , Zhou Huimin , Jia Li 
Published: December 2016

AbstractSialylation is one of the altered glycosylation patterns associated with cancer progression. In this study, we investigated the N-glycan profiles of breast cancer patients and cell lines to reveal sialylation associated with breast cancer progression, and provided new evidences of miRNA-mediated sialylation. MALDI-TOF MS analysis revealed that N-glycans found in breast cancer tissues and breast cancer cell MDA-MB-231 featured increased levels of sialylation compared with adjacent tissues and normal breast epithelial cell MCF-10A. The expressional profiles of 20 sialyltransferase genes were then analyzed and found significantly different comparing breast cancer samples with adjacent tissues, and two breast cancer cell lines MDA-MB-231 and MCF-7 with different metastatic potential and MCF-10A cells. Tumor tissues and highly metastatic breast cancer cell line MDA-MB-231 exhibited higher levels of ST8SIA4. Knocking down ST8SIA4 in breast cancer cell lines significantly inhibited their malignant behaviors including cell proliferation and invasion in a sialyltransferase-dependent manner. By applying bioinformatic approaches for the prediction of miRNA targeting 3′-UTR of ST8SIA4, we identified ST8SIA4 as one of the miR-26a/26b-targeted genes. Further data analysis revealed the inversely related expression of ST8SIA4 and miR-26a/26b in breast cancer cells, tumor tissues and corresponding adjacent tissues. The ability of miR-26a/26b to interact specifically with and regulate the 3′-UTR of ST8SIA4 was demonstrated via a luciferase reporter assay. The forced expression of miR-26a/26b was able to induce a decrease of ST8SIA4 level and also to affect breast cancer cells progression, while altered expression of ST8SIA4 in breast cancer cells modulated progression upon transfection with miR-26a/26b mimics or inhibiter. Taken together, these results indicate that changes in the glycosylation patterns and sialylation levels may be useful markers of the progression of breast cancer, as well as miR-26a/26b may be widely involved in the regulation of sialylation machinery by targeting ST8SIA4.
Speedy A–Cdk2 binding mediates initial telomere–nuclear envelope attachment during meiotic prophase I independent of Cdk2 activation
Authors: Tu Zhaowei , Bayazit Mustafa Bilal , Liu Hongbin , Zhang Jingjing , Busayavalasa Kiran , Risal Sanjiv , Shao Jingchen , Satyanarayana Ande , Coppola Vincenzo , Tessarollo Lino , Singh Meenakshi , Zheng Chunwei , Han Chunsheng , Chen Zijiang , Kaldis Philipp , Gustafsson Jan-Åke , Liu Kui 
Published: December 2016

Significance
In meiotic prophase I, telomere attachment to the nuclear envelope is a prerequisite for subsequent prophase events, such as homologous pairing and recombination. In this study, we show that Speedy A, a noncanonical activator of cyclin-dependent kinases (Cdks), is essential for telomere attachment to the nuclear envelope in mice. We have identified a telomere localization domain in Speedy A, which covers the protein’s distal N-terminus and Cdk2-binding Ringo domain but excludes its Cdk-activation domain. Furthermore, we found that the binding of Cdk2 to Speedy A is essential for Cdk2’s localization to telomeres. Our results suggest that Speedy A–Cdk2 binding might mediate the initial assembly of the meiotic telomere complex, a process that is independent of Cdk2 activation.
Modelling larval dispersal of Pecten maximus in the English Channel: a tool for the spatial management of the stocks
Authors: Nicolle Amandine , Moitié Roderic , Ogor Julien , Dumas Franck , Foveau Aurélie , Foucher Eric , Thiébaut Eric 
Published: December 2016

AbstractThe great scallop Pecten maximus supports one of the most important and valuable commercial fisheries around the British Isles and in the northwest of France, but the resource is mainly managed at the scale of each local fishing ground through a combination of European, national and local measures. To analyse the larval dispersal pathways and connectivity patterns among fishing grounds of the great scallop in the Celtic Sea and the English Channel, a particle tracking model was developed. The model combined a 3D physical circulation model that simulated currents and temperature fields and a scallop larval submodel that took into account a temperature-dependent planktonic larval duration and an active vertical swimming behaviour. Due to the lack of stock assessment at the regional scale, the location of the main fishing grounds was established by combining different sources (e.g. grey literature, unpublished scientific surveys, vessel monitoring data, fishermen) while the spawning biomass of each stock was estimated from landings data. Results indicated that each local stock could not be considered as a single independent management unit and that all stocks except that of the Bay of Brest were connected to neighbouring stocks, suggesting that the management should be defined in a metapopulation context. Three major groups of strongly interconnected stocks including two or three stocks exhibiting high retention and self-recruitment rates and some peripheral stocks with a low self-recruitment rate were defined: the North Brittany and Channel Islands, the eastern English Channel, and the SW of England. Our results were discussed in terms of the definition of management units in comparison with genetic and phenotypic data, and in terms of resource management in a transnational context.
Tubulopapillary Cystic Adenoma With Apocrine Differentiation: A Unifying Concept for Syringocystadenoma Papilliferum, Apocrine Gland Cyst, and Tubular Papillary Adenoma
Authors: Ansai Shin-ichi , Anan Takashi , Fukumoto Takaya , Saeki Hidehisa 
Published: December 2016

Abstract:
Syringocystadenoma papilliferum (SCAP), apocrine gland cyst (AGC, also called apocrine hidrocystoma or apocrine cystadenoma), and tubular papillary adenoma (TPA) with apocrine differentiation are defined as proliferations of apocrine epithelium with myoepithelial cells. At Sapporo Dermatopathology Institute, we retrieved 308 benign neoplastic lesions diagnosed as SCAP, AGC, or TPA and combinations of these entities. Among the 308 lesions, 202 (66%) exhibited features of only one type, of which 144 (47%) were AGC, 39 (13%) were TPA, and 19 (6%) were SCAP. The other 106 lesions (34%) had features of 2 or more types, including 56 lesions that were AGC + TPA (18%), 2 that were AGC + SCAP (1%), 34 that were TPA + SCAP (11%), and 14 that were AGC + TPA + SCAP (5%). The most frequent site of these lesions was the face (56%), followed by the scalp (13%). Lesions with the features of AGC were more frequently found on the face, especially the periocular region, than at other sites. TPA lesions were more frequent on the face and scalp than at other sites, whereas SCAP lesions were preferentially found on the face, scalp, and trunk. We also retrieved clinicopathological data and other information. We propose a unifying concept for AGC, TPA, and SCAP. Approximately one-third of these lesions are composite entities with the features of 2 or 3 different tumors, and we propose calling such tumors tubulopapillary cystic adenoma with apocrine differentiation.
Malignant Melanoma in Association With a Thymic Nevus in a Patient With a Giant Congenital Nevus
Authors: Shvartser-Beryozkin Yulia , Yakobson Alexander , Benharroch Daniel , Saute Milton , Feinmesser Meora 
Published: December 2016

Abstract:
Nevi and melanocytic proliferations are known to appear in multiple extracutaneous sites, including lymph nodes and meninges. We report a case of an anterior mediastinal mass in a patient with a giant congenital nevus and neurofibromatosis type I. Histologically, the tumor was found to be a malignant melanoma in the thymus arising in association with a nevus that involved most of the thymic tissue. There was no sign of cutaneous melanoma on skin examination. We suggest that the tumor originated from the benign nevus in the thymus, a rare extracutaneous location for nevi and malignant melanoma.