bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aptamers against Cells Overexpressing Glypican 3 from Expanded Genetic Systems Combined with Cell Engineering and Laboratory Evolution
Authors: Zhang Liqin , Yang Zunyi , Le Trinh Thu , Teng I‐Ting , Wang Sai , Bradley Kevin M. , Hoshika Shuichi , Wu Qunfeng , Cansiz Sena , Rowold Diane J. , McLendon Christopher , Kim Myong‐Sang , Wu Yuan , Cui Cheng , Liu Yuan , Hou Weijia , Stewart Kimberly , Wan Shuo , Liu Chen , Benner Steven A. , Tan Weihong 
Published: September 2016

AbstractLaboratory in vitro evolution (LIVE) might deliver DNA aptamers that bind proteins expressed on the surface of cells. In this work, we used cell engineering to place glypican 3 (GPC3), a possible marker for liver cancer theranostics, on the surface of a liver cell line. Libraries were then built from a six‐letter genetic alphabet containing the standard nucleobases and two added nucleobases (2‐amino‐8H‐imidazo[1,2‐a][1,3,5]triazin‐4‐one and 6‐amino‐5‐nitropyridin‐2‐one), Watson–Crick complements from an artificially expanded genetic information system (AEGIS). With counterselection against non‐engineered cells, eight AEGIS‐containing aptamers were recovered. Five bound selectively to GPC3‐overexpressing cells. This selection–counterselection scheme had acceptable statistics, notwithstanding the possibility that cells engineered to overexpress GPC3 might also express different off‐target proteins. This is the first example of such a combination.
Linear Chains of Magnetic Ions Stacked with Variable Distance: Ferromagnetic Ordering with a Curie Temperature above 20 K
Authors: Friedländer Stefan , Liu Jinxuan , Addicoat Matt , Petkov Petko , Vankova Nina , Rüger Robert , Kuc Agnieszka , Guo Wei , Zhou Wencai , Lukose Binit , Wang Zhengbang , Weidler Peter G. , Pöppl Andreas , Ziese Michael , Heine Thomas , Wöll Christof 
Published: September 2016

AbstractWe have studied the magnetic properties of the SURMOF‐2 series of metal–organic frameworks (MOFs). Contrary to bulk MOF‐2 crystals, where Cu2+ ions form paddlewheels and are antiferromagnetically coupled, in this case the Cu2+ ions are connected via carboxylate groups in a zipper‐like fashion. This unusual coupling of the spin
ions within the resulting one‐dimensional chains is found to stabilize a low‐temperature, ferromagnetic (FM) phase. In contrast to other ordered 1D systems, no strong magnetic fields are needed to induce the ferromagnetism. The magnetic coupling constants describing the interaction between the individual metal ions have been determined in SQUID experiments. They are fully consistent with the results of ab initio DFT electronic structure calculations. The theoretical results allow the unusual magnetic behavior of this exotic, yet easy‐to‐fabricate, material to be described in a detailed fashion.
A Kinetic Self‐Sorting Approach to Heterocircuit [3]Rotaxanes
Authors: Neal Edward A. , Goldup Stephen M. 
Published: September 2016

AbstractIn this proof‐of‐concept study, an active‐template coupling is used to demonstrate a novel kinetic self‐sorting process. This process iteratively increases the yield of the target heterocircuit [3]rotaxane product at the expense of other threaded species.
ESOA modified unsaturated polyester hybrid networks: A new perspective
Authors: Panda Shivkumari , Mohanty Priyabrata , Behera Dibakar , Kumar Bastia Tapan 
Published: September 2016

ABSTRACTHybrid polymer networks based on unsaturated polyester (UPE) and epoxidized soybean oil acrylate (ESOA) were synthesized by reactive blending through free radical addition polymerization reaction. ESOA was prepared by acrylation of epoxidized soybean oil (ESO). The physical, mechanical, thermal and electrical properties of the cured blends were compared with the neat resin. ESOA resin bearing reactive functional groups showed good miscibility and compatibility with the UPE resin. The co‐cured resin showed substantial upgrading in the toughness, impact resistance, thermal properties, and downgrading brittleness up to the addition of 20 wt % of ESOA content. The muddled phase structure was corroborated by Fourier transform infrared spectroscopy, scanning electron microscope, and transmission electron microscopy and proved the formation of excellent hybrid polymer network. An improvement in overall properties has been achieved without seriously affecting any other properties. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44345.
Observable Degree Analysis to Match Estimation Performance for Wireless Tracking Networks
Authors: Ge Quanbo , Ma Jinyan , Chen Shaodong , Wang Yongting , Bai Liang 
Published: September 2016

AbstractState estimation suffers some new challenging problems when wireless sensor networks (WSNs) are used in mobile target tracking systems. An important problem among them is low observability, which makes it necessary to further study performance of tracking estimators from an observable degree point of view. This paper studies observable degree analysis (ODA) to formulate the estimator performance for a kind of wireless tracking filter. For a kind of time‐varying Kalman estimator, an improved ODA method is proposed by using weighted least square, Cauchy Schwarz inequality and normalization processing. The main contributions are that the relation is firstly analytically established between the observable degree and the estimation performance, and finally the observable degree is normalized in [0,1]. Meanwhile, observable degrees are both given for local state components and the global state. Thereby, we have the conclusion that higher observable degree corresponds to better estimation performance of the Kalman filter. Accordingly, it is potentially hopeful to achieve an effective function to study estimation performance of tracking estimators by directly using the observable degree. Simulation is provided to verify the results on real‐time and generality, completeness and consistency/matching of the proposed observable degree analysis.
Pre‐incubation with cyclosporine A potentiates its inhibitory effects on pitavastatin uptake mediated by recombinantly expressed cynomolgus monkey hepatic organic anion transporting polypeptide
Authors: Takahashi Tsuyoshi , Ohtsuka Tatsuyuki , Uno Yasuhiro , Utoh Masahiro , Yamazaki Hiroshi , Kume Toshiyuki 
Published: September 2016

AbstractCyclosporine A, an inhibitor of hepatic organic anion transporting polypeptides (OATPs), reportedly increased plasma concentrations of probe substrates, although its maximum unbound blood concentrations were lower than the experimental half‐maximal inhibitory (IC50) concentrations. Pre‐incubation with cyclosporine A in vitro before simultaneous incubation with probes has been reported to potentiate its inhibitory effects on recombinant human OATP‐mediated probe uptake. In the present study, the effects of cyclosporine A and rifampicin on recombinant cynomolgus monkey OATP‐mediated pitavastatin uptake were investigated in pre‐ and simultaneous incubation systems. Pre‐incubation with cyclosporine A, but not with rifampicin, decreased the apparent IC50 values on recombinant cynomolgus monkey OATP1B1‐ and OATP1B3‐mediated pitavastatin uptake. Application of the co‐incubated IC50 values toward R values (1 + [unbound inhibitor]inlet to the liver, theoretically maximum/inhibition constant) in static models, 1.1 in monkeys and 1.3 in humans, for recombinant cynomolgus monkey and human OATP1B1‐mediated pitavastatin uptake might result in the poor prediction of drug interaction magnitudes. In contrast, the lowered IC50 values after pre‐incubation with cyclosporine A provided better prediction with R values of 3.9 for monkeys and 2.7 for humans when the estimated maximum cyclosporine A concentrations at the inlet to the liver were used. These results suggest that the enhanced inhibitory potential of perpetrator medicines by pre‐incubation on cynomolgus monkey OATP‐mediated pitavastatin uptake in vitro could be of value for the precise estimation of drug interaction magnitudes in silico, in accordance with the findings from pre‐administration of inhibitors on pitavastatin pharmacokinetics validated in monkeys. Copyright © 2016 John Wiley & Sons, Ltd.
Interactions of donor sources and media influence the histo‐morphological quality of full‐thickness skin models
Authors: Lange Julia , Weil Frederik , Riegler Christoph , Groeber Florian , Rebhan Silke , Kurdyn Szymon , Alb Miriam , Kneitz Hermann , Gelbrich Götz , Walles Heike , Mielke Stephan 
Published: September 2016

AbstractHuman artificial skin models are increasingly employed as non‐animal test platforms for research and medical purposes. However, the overall histopathological quality of such models may vary significantly. Therefore, the effects of manufacturing protocols and donor sources on the quality of skin models built‐up from fibroblasts and keratinocytes derived from juvenile foreskins is studied. Histo‐morphological parameters such as epidermal thickness, number of epidermal cell layers, dermal thickness, dermo‐epidermal adhesion and absence of cellular nuclei in the corneal layer are obtained and scored accordingly. In total, 144 full‐thickness skin models derived from 16 different donors, built‐up in triplicates using three different culture conditions were successfully generated. In univariate analysis both media and donor age affected the quality of skin models significantly. Both parameters remained statistically significant in multivariate analyses. Performing general linear model analyses we could show that individual medium‐donor‐interactions influence the quality. These observations suggest that the optimal choice of media may differ from donor to donor and coincides with findings where significant inter‐individual variations of growth rates in keratinocytes and fibroblasts have been described. Thus, the consideration of individual medium‐donor‐interactions may improve the overall quality of human organ models thereby forming a reproducible test platform for sophisticated clinical research.
Biological activity of peptide‐conjugated polyion complex matrices consisting of alginate and chitosan
Authors: Fujimori Chikara , Kumai Jun , Nakamura Kyotaro , Gu Yingzi , Katagiri Fumihiko , Hozumi Kentaro , Kikkawa Yamato , Nomizu Motoyoshi 
Published: September 2016

AbstractPeptide‐conjugated polysaccharide matrices using bioactive laminin‐derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide‐polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde‐alginate was synthesized by oxidization of alginate using NaIO4, and then, reacted with Cys‐peptides. Next, the peptide‐alginate solution was added to a chitosan‐coated plate, and the peptide‐polyion complex matrices (peptide‐PCMs) were prepared. The peptide‐PCMs using an integrin αvβ3‐binding peptide (A99a: ALRGDN, mouse laminin α1 chain 1145‐1150) and an integrin α2β1‐binding peptide (EF1XmR: RLQLQEGRLHFXFD, X = Nle, mouse laminin α1 chain 2751‐2763) showed strong cell attachment activity in a dose‐dependent manner. When we examined the effect of various spacers on the biological activity of A99a‐PCM, hydrophobic and long spacers enhanced the cell attachment activity. Further, the A99a‐PCM with the spacers strongly promoted neurite outgrowth. The polyion complex method is an easy way to obtain insolubilized matrix and is widely applicable for various polysaccharides. The peptide‐PCM is useful as a biomaterial for cell and tissue engineering.
Self‐Assembly of a [2]Pseudorotaxane by an Inchworm‐Motion Mechanism
Authors: Meng Zheng , Wang Bo‐Yang , Xiang Jun‐Feng , Shi Qiang , Chen Chuan‐Feng 
Published: September 2016

AbstractThe threading of biomolecules through pores or channels in membranes is important to validate the physiological activities of cells. To aid understanding of the controlling factors required for the translocation in space with confined size and distorted conformation, it is desirable to identify experimental systems with minimized complexity. We demonstrate the mechanism of a linear guest L1 threading into a tris(crown ether) host TC with a combinational distorted cavity to form a triply interlocked [2]pseudorotaxane 3in‐[L1⊂TC]. An inchworm‐motion mechanism is proposed for the process. For the forward‐threading steps that lead to the formation of higher‐order interlocked species, guest L1 must adopt a bent conformation to find the next crown ether cavity. Two simplified models are applied to investigate the self‐assembly dynamic of 3in‐[L1⊂TC]. Kinetic NMR spectroscopic and molecular dynamics (MD) studies show that formation of the singly penetrated species is fast, whereas formation of the doubly and triply threaded species is several orders of magnitude slower. During threading the freedom of both the guest L1 and host TC gradually decrease due to their interactions. This results in a significant entropy effect for the threading dynamic, which is also observed for the threading of a biomolecular chain through a channel.
Obtaining miRNA‐Target Interaction Information from miRWalk2.0
Authors: Parveen Alisha , Gretz Norbert , Dweep Harsh 
Published: September 2016

AbstractmiRWalk2.0 (http://zmf.umm.uni‐heidelberg.de/mirwalk2) is a freely accessible, regularly updated comprehensive archive supplying the largest available collection of predicted and experimentally verified miRNA‐target interactions, with various novel and unique features to assist the scientific community. Approximately 949 million interactions between 11,748 miRNAs, 308,700 genes, and 68,460 lncRNAs are documented in miRWalk2.0 with 5,146,217 different kinds of identifiers to offer a one‐stop site to collect an abundance of information. This article describes a schematic workflow on how to obtain miRNA‐target interactions from miRWalk2.0. © 2016 by John Wiley & Sons, Inc.
Social‐ecological outcomes in recreational fisheries: the interaction of lakeshore development and stocking
Authors: Ziegler Jacob P. , Golebie Elizabeth J. , Jones Stuart E. , Weidel Brian C. , Solomon Christopher T. 
Published: September 2016

AbstractMany ecosystems continue to experience rapid transformations due to processes like land use change and resource extraction. A systems approach to maintaining natural resources focuses on how interactions and feedbacks among components of complex social‐ecological systems generate social and ecological outcomes. In recreational fisheries, residential shoreline development and fish stocking are two widespread human behaviors that influence fisheries, yet emergent social‐ecological outcomes from these potentially interacting behaviors remain under explored. We applied a social‐ecological systems framework using a simulation model and empirical data to determine whether lakeshore development is likely to promote stocking through its adverse effects on coarse woody habitat and thereby also on survival of juvenile and adult fish. We demonstrate that high lakeshore development is likely to generate dependency of the ecosystem on the social system, in the form of stocking. Further, lakeshore development can interact with social‐ecological processes to create deficits for state‐level governments, which threatens the ability to fund further ecosystem subsidies. Our results highlight the value of a social‐ecological framework for maintaining ecosystem services like recreational fisheries.
Modeling the spatial behavior of the meteorological drivers' effects on extreme ozone
Authors: Russell Brook T. , Cooley Daniel S. , Porter William C. , Heald Colette L. 
Published: September 2016

At its most extreme levels, ground‐level ozone is most harmful to human health, and meteorological conditions play a critical role in such episodes. In this work, our aim is to better understand how the primary meteorological drivers' effects on extreme ozone vary over the Southeast and Mid‐Atlantic region of the USA.We employ a model based on a bivariate extreme value framework that finds the linear combination of a set of meteorological covariates that has the strongest tail dependence with ground‐level ozone. In order to gain knowledge about the spatial behavior of the meteorological drivers, we spatially model the coefficients, which relate the covariates to extreme ozone. Because inference for our extreme value model is not likelihood based, we utilize a two‐stage modeling procedure: first, estimating the coefficients in our extreme value model and their associated uncertainties and then using these to fit a multivariate spatial process.We analyze data from 160 air quality stations located in the Environmental Protection Agency Regions 3 and 4, producing estimated spatial surfaces via the fitted spatial process and co‐kriging. We find that the relative contribution of the driving meteorological variables to extreme ozone differs between the northern and southern portions of the study region. For instance, we find that air temperature is more important in the northern portion of the region, while low humidity is more influential in the southern portion of the region.
Tissue-specific bioconcentration and biotransformation of cypermethrin and chlorpyrifos in a native fish (Jenynsia multidentata) exposed to these insecticides singly and in mixtures
Authors: Bonansea Rocío Inés , Marino Damián J.G. , Bertrand Lidwina , Wunderlin Daniel A. , Amé María Valeria 
Published: September 2016

Abstract

The aim of the present study was to evaluate the accumulation of cypermethrin and chlorpyrifos when the fish Jenynsia multidentata was exposed to these pesticides singly and in technical and commercial mixtures. Adult female fish were exposed over 96 h to 0.04 μg/L of cypermethrin; 0.4 μg/L of chlorpyrifos; 0.04 μg/L of cypermethrin + 0.4 μg/L of chlorpyrifos in a technical mixture; and 0.04 μg/L of cypermethrin + 0.4 μg/L of chlorpyrifos in a mixture of commercial products. Fish exposed to cypermethrin accumulated this compound only in muscle, probably because of the low biotransformation capacity of this organ and the induction of cytochrome P4501A1 (CYP1A1) expression in the liver. The accumulation of chlorpyrifos occurred in fish exposed to the insecticide (intestine &gt; liver &gt; gills) even when these fish had higher gluthatione-S-transferase (GST) activity in gills and P-glycoprotein (P-gp) expression in the liver, compared with the control. Fish exposed to the technical mixture showed cypermethrin accumulation (liver &gt; intestine &gt; gills) with higher levels than those measured in fish after only cypermethrin exposure. Higher expression levels of CYP1A1 in the liver were also observed compared with the Control. Fish exposed to the commercial mixture accumulated both insecticides (cypermethrin: intestine &gt; gills and chlorpyrifos: liver &gt; intestine &gt; gills &gt; muscle). In the organs where accumulation occurred, biotransformation enzymes were inhibited. Consequently, the commercial formulation exposure provoked the highest accumulation of cypermethrin and chlorpyrifos in J. multidentata, possibly associated with the biotransformation system inhibition. Environ Toxicol Chem 2017;36:1764–1774. © 2016 SETAC
Real‐time estimation of dynamic functional connectivity networks
Authors: Monti Ricardo Pio , Lorenz Romy , Braga Rodrigo M. , Anagnostopoulos Christoforos , Leech Robert , Montana Giovanni 
Published: September 2016

AbstractTwo novel and exciting avenues of neuroscientific research involve the study of task‐driven dynamic reconfigurations of functional connectivity networks and the study of functional connectivity in real‐time. While the former is a well‐established field within neuroscience and has received considerable attention in recent years, the latter remains in its infancy. To date, the vast majority of real‐time fMRI studies have focused on a single brain region at a time. This is due in part to the many challenges faced when estimating dynamic functional connectivity networks in real‐time. In this work, we propose a novel methodology with which to accurately track changes in time‐varying functional connectivity networks in real‐time. The proposed method is shown to perform competitively when compared to state‐of‐the‐art offline algorithms using both synthetic as well as real‐time fMRI data. The proposed method is applied to motor task data from the Human Connectome Project as well as to data obtained from a visuospatial attention task. We demonstrate that the algorithm is able to accurately estimate task‐related changes in network structure in real‐time.Hum Brain Mapp 38:202–220, 2017. ©2016 Wiley Periodicals, Inc.
Immediate referral to colposcopy versus cytological surveillance for low‐grade cervical cytological abnormalities in the absence of HPV test: A systematic review and a meta‐analysis of the literature
Authors: Kyrgiou Maria , Kalliala Ilkka , Mitra Anita , Ng Ka Ying Bonnie , Raglan Olivia , Fotopoulou Christina , Martin‐Hirsch Pierre , Paraskevaidis Evangelos , Arbyn Marc 
Published: September 2016

We performed a systematic review and meta‐analysis to explore the optimum management strategy for women with atypical squamous cells of undetermined significance (ASCUS/borderline) or low‐grade squamous intra‐epithelial lesions (LSIL/mild dyskaryosis) cytological abnormalities at primary screening in the absence of HPV DNA test. We searched MEDLINE, EMBASE and CENTRAL and included randomised controlled trials comparing immediate colposcopy to cytological surveillance in women with ASCUS/LSIL. The outcomes of interest were occurrence of different histological grades of cervical intraepithelial neoplasia (CIN) and default rates during follow‐up. Pooled risk ratios (RR) and 95% confidence intervals (CI) were calculated using a random‐effect model and with inverse variance weighting. Interstudy heterogeneity was assessed using I2 statistics. Six RCTs were included. Immediate colposcopy significantly increased detection of unimportant abnormalities as opposed to repeat cytology (koilocytosis: 32 vs. 21%, RR: 1.49, 95% CI = 1.17–1.90); CIN1: 21 vs. 8%, RR: 2.58, 95% CI = 1.69–3.94). Although immediate colposcopy detected CIN2, CIN2+, and CIN3+ earlier than cytology, the differences were no longer observed at 24 months (CIN3+: 10.3 vs.11.9%, RR: 1.02, 95% CI = 0.53–1.97), with significant interstudy heterogeneity (p < 0.001, I2 = 93%). Default risk was significantly higher for repeat cytology (6 months: 6.3 vs. 13.3%, RR: 3.85, 95% CI = 1.27–11.63; 12 months: 6.3 vs. 14.8%, RR: 6.39, 95% CI = 1.24–32.95; 24 months: 0.9 vs. 16.1%, RR: 19.1, 95% CI = 9.02–40.4). Detection of CIN2+ for cytological surveillance over two years is similar to that of immediate colposcopy, although patients may default. Colposcopy may be first choice when good compliance is not assured, but may increase detection of insignificant lesions. This emphasizes the need for a reflex triage test to distinguish women who need diagnostic work‐up from those who can return to routine recall.