bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anais da Jornada da Residência Médica em Cirurgia da Mão do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (HCFMRP-USP) - 2024
Authors: Rezende Luis Guilherme Rosifini Alves 
Published: October 2024

No dia 17 de outubro de 2024, sábado, realizou-se no Anfiteatro Prof. Marcondes, no 11º Andar do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (HCFMRP-USP) a Jornada da Residência Médica em Cirurgia da Mão do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto (HCFMRP-USP) – Edição de 2024. Este evento teve como objetivo promover e divulgar os conhecimentos das diferentes linhas de pesquisa da Residência Médica e do Grupo de Cirurgia da Mão, Microcirurgia e Membro Superior (do Complexo HC-FMRP-USP), representada pelos doze Médicos Residentes do Programa de Residência Médica em Cirurgia da Mão do HCFMRP-USP. As apresentações contemplaram os trabalhos que são considerados como pré-requisitos para a atividade acadêmica e como pré-requisito para o “Exame de Suficiência para obtenção do Título de Especialista em Cirurgia da Mão”, aplicado pela Sociedade Brasileira pertinente, conforme normativas e regulamentos internos. Os trabalhos foram avaliados previamente, apresentados e discutidos com o Coordenador e Responsável pelo Programa de Residência Médica em Cirurgia da Mão, e equipe de Preceptores, além de contar com a participação híbrida de convidados selecionados, membros da equipe e egressos do Programa de Residência Médica em Cirurgia da Mão do Complexo HC-FMRP-USP. Assim, os Médicos Residentes, de uma subespecialidade que permite atribuição de RQE pelo Conselho Federal de Medicina (CFM), passa a demonstrar a oportunidade de ensino e competências no formato de um centro formador tradicional, e a contribuição dos projetos e pesquisas que beneficiarão inúmeros pacientes, prestando assistência e auxílio, além de fortalecer e auxiliar protocolos internos utilizados por nossa instituição. O evento, também mostrou o conhecimento e preparo para a carreira, cujos Médicos Residentes passarão a se inserir no mercado de trabalho. Jornada da Residência Médica em Cirurgia da Mão do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto (HCFMRP-USP) – Edição de 2024, passará a ocorrer como evento anual, estimulando o Ensino e Pesquisa. Prof. Dr. Luis Guilherme Rosifini Alves Rezende Professor do Departamento de Ortopedia e Anestesiologia da FMRP-USP/ Responsável pela Divisão de Cirurgia da Mão, Microcirurgia e Cirurgia do Membro Superior HCFMRP-USP /Responsável pelo Programa de Residência Médica em Cirurgia da Mão do HCFMRP-USP
Advanced machine learning for predicting groundwater decline and drought in the Rabat–Salé–Kénitra region, Morocco
Authors: Elmotawakkil Abdessamad , Enneya Nourddine 
Published: October 2024

ABSTRACT

The Rabat–Salé–Kénitra region of Morocco faces critical groundwater challenges due to increasing demands from population growth, agricultural expansion, and the impacts of prolonged droughts and climate change. This study employs advanced machine learning models, including artificial neural networks (ANN), gradient boosting (GB), support vector regression (SVR), decision tree (DT), and random forest (RF), to predict groundwater storage variations. The dataset encompasses hydrological, meteorological, and geological factors. Among the models evaluated, RF demonstrated superior performance, achieving a mean squared error (MSE) of 484.800, a root mean squared error (RMSE) of 22.018, a mean absolute error (MAE) of 14.986, and a coefficient of determination (R2) of 0.981. Sensitivity analysis revealed significant insights into how different models respond to variations in key environmental factors such as evapotranspiration and precipitation. Prophet was also integrated for its ability to handle seasonality in time-series data, further enhancing prediction reliability. The findings emphasize the urgent need to integrate advanced predictive models into groundwater management to address groundwater depletion and ensure sustainable water resources amid rising drought conditions. Policymakers can use these models to regulate extraction, promote water-saving technologies, and enhance recharge efforts, ensuring the sustainability of vital groundwater resources for future generations.
Measurement and thermal characterization of graywater discharge events for house-central collection systems in the context of heat recovery
Authors: Mehling Simon , Hörnlein Stefanie , Schnabel Tobias , Beier Silvio , Londong Jörg 
Published: October 2024

ABSTRACT
Within this work, a detailed measurement with a temporal resolution of 3 s of flow rate and temperature of a house-central graywater system was carried out. Individual discharge events could be identified and assigned to specific types of use via user surveys and current measurements. Due to the consistent recording of measurement errors, the accuracy of the determined data could be improved. Error rates of approximately 0–30% were determined, depending on the tab. Thus, accurate statements on the existing temperature distribution for the graywater of specific tabs could be determined. Tab-specific temperature distributions were calculated with ranges of 16–50 °C and an overall mean value of approximately 28 °C. Furthermore, a novel data basis could be provided by evaluating the time-related rate of change of temperatures. This enables more precise simulations to be carried out. For some tabs, significant differences to previous assumptions became apparent. The high temporal resolution of the data also enables simulative considerations that go beyond previous longer-scale energy balances.
Wastewater desalination for irrigation in inland regions
Authors: Sanciolo Peter , Haby Carl , Sharma Ashok K. , Muthukumaran Shobha , Gray Stephen 
Published: October 2024

ABSTRACT
Desalination using membrane processes is increasingly being implemented in coastal regions of the world to meet the water needs of the growing population. The use of this water treatment option to meet the growing water needs in inland regions, however, is impeded by the cost and regulatory challenges associated with responsible disposal of the concentrate brine. For inland regions of Australia, the use of evaporation ponds (EPs) is the only available brine management option. The high cost associated with construction and the high land requirements for EPs necessitates low waste brine volume production. This desktop study investigated the feasibility of achieving very high water recoveries and very low waste brine volumes in the reverse osmosis desalination of municipal wastewater for irrigation of crops near the inland town of Horsham, Victoria, Australia. Interstage lime and soda ash softening was selected as the means of removal of the scalants that prevent the achievement of high water recovery. It is hoped that this study will provide valuable information to aid in the planning of water reclamation operations for agricultural applications in inland areas at a time of increasing demand for water for food production and forecasts of population growth and climate change.
AFROCENTRICIDADE
Authors: Fraga Celestino Livia 
Published: October 2024

Este artigo possui como base a afrocentricidade buscando analisar as percepções de espaço e tempo sagrado nas comunidades dos terreiros de candomblé. Em um processo de forte dominação colonial em que vivemos, a afrocentricidade é uma abordagem necessária por colocar o pensamento africano, do continente e da diáspora, no centro do discurso e não mais como margem. Assim, as pesquisas que tratam das cosmopercepções afrodiaspóricas abrem possibilidades de perceber o mundo com outros olhares, outros sentidos de espaço e tempo. Com referências pautadas na afrocentricidade e, em minha vivência enquanto pessoa de axé, este artigo trata de maneira introdutória algumas discussões sobre espaço e tempo para as comunidades de terreiro. No candomblé as noções de espaço e tempo sagrados são vividas a partir das cosmopercepções afrodiaspóricas percebendo o mundo e a relação com as pessoas a partir de outras percepções e sentidos atraleadas a ancestralidade, natureza, comunidade e axé.
Characterization of Luciferase from Photorhabdus kayaii and its Application for In vivo Imaging Studies in Mice
Authors: Tarahomjoo Shirin , Ebrahimi Laleh , Valishahavaz Fatemeh , Taghdiri Maryam , Banihashemi Reza 
Published: October 2024

Background:
Bioluminescence, or the production of light by luciferases, is the basis of
a well-known reporter technology. A quick way to study the efficacy of antimicrobial drugs and
vaccines is in vivo bioluminescence imaging (BLI). Photorhabdus spp. represent the only terrestrial
group of bioluminescent bacteria. The luciferase obtained from Photorhabdus luminescence
has been widely used in BLI studies. However, little information is available about the functions
of luciferases obtained from other members of this genera.

Objective:
This study aimed to evaluate the applicability of the luciferase obtained from Photorhabdus
kayaii for BLI studies.

Methods:
P. kayaii starE, an Iranian isolate of P. kayaii, was cultivated on NBTA agar plates. The
resulting colonies were cultured on McConkey agar to determine the bacterial phase. Bioluminescence
emission was measured using a multimode reader. The luciferase genes of this bacterium
were sequenced following the PCR amplification, and the corresponding amino acid sequences
were determined. The luciferase tertiary structure was then obtained from the TACOS web server
and compared to that of P. luminescence in CE software. The lux operon encoding the luciferase
(luxA and luxB genes) and substrate synthesis complex was cloned and expressed in Escherichia
coli BL21 (DE3) using the pBBR1MCS2_START vector. The luminescence emission during the
growth was examined. Moreover, the effects of pH and sodium deoxycholate (bile salt) on bioluminescence
emission were investigated. Appropriate conditions for the use of bioluminescent E.
coli for BLI studies in mice were demonstrated in terms of cell numbers and injection routes.

Results:
The bacterium was luminescent and in phase I. Its luciferase monomers (α and β) shared
100% amino acid homology with P. kayaii M-HU2 and more than 92% with P. luminescence. Tertiary
structures of the luciferase monomers were 93%- 95% identical to those of P. luminescence.
The lux operon was expressed in E. coli, and the maximum bioluminescence signal was observed
during the decelerating phase of growth. The bioluminescence at different pH values correlated
with the cell survival. The luminescence was emitted by cells exposed to the bile salt. A strong bioluminescent
signal was emitted from mice after subcutaneous injection of bioluminescent E. coli
at 107 CFU. However, no signals were emitted from mice that were administered the same cell
number via intraperitoneal injection. A 2.5-fold increase in the cell number resulted in bioluminescence
detection in the abdomen of mice after intraperitoneal injection and a 3.22-fold increase in
signal intensity after subcutaneous injection.

Conclusion:
These results demonstrated the usefulness of P. kayaii luciferase for BLI studies.
Multiple Machine Learning Models, Molecular Subtyping and Singlecell Analysis Identify PANoptosis-related Core Genes and their Association with Subtypes in Crohn’s Disease
Authors: Chen Yi , Zhang Lu , Huang Wan-Ying , He Rong-Quan , Huang Zhi-Guang , Li Hui , Song Rui , Zhang Jia-Wei , He Juan , Chen Gang 
Published: October 2024

Background:
PANoptosis plays an important role in many inflammatory diseases.
However, there are no reports on the association between PANoptosis and CD.

Materials and Methods:
This study used five machine learning algorithms - least absolute
shrinkage and selection operator, support vector machine, random forest, decision
tree and Gaussian mixture models - to construct CD’s PANoptosis signature. Unsupervised
hierarchical clustering analysis was used to identify PANoptosis-associated subgroups
of CD. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes
and Genomes (KEGG) pathway analysis, Gene Set Enrichment Analysis (GSEA) and
Gene Set Variation Analysis (GSVA) were conducted to compare the PANoptosis-associated
subgroups of CD among the potential biological mechanisms. Single sample
GSEA was used to assess immune microenvironmental differences among the subgroups.
The potential role of PANoptosis in CD was further explored using single-cell
RNA-Seq (scRNA-Seq) for PANoptosis scoring, differential analysis, pseudotime analysis,
cellular communication analysis and weighted gene co-expression network analysis
(WGCNA) analysis.

Results:
CD’s PANoptosis signature consisted of seven genes: CEACAM6, CHP2,
PIK3R1, CASP10, PSMB1, PSMB8 and UBC. The PANoptosis signature in multiple cohorts
had a strong ability to recognise CD. The levels of immune cell infiltration and the
vigour of the immune responses significantly varied between the two subpopulations of
CD associated with PANoptosis. Multiple lines of evidence from the GO, KEGG,
GSEA, GSVA, scRNA-Seq and WGCNA analyses suggested that I-kappaB kinase/NF-
kappaB signalling, mitogen-activated protein kinase (MAPK), leukocyte activation
and leukocyte migration were mechanisms closely associated with PANoptosis in CD.

Conclusion:
This study is the first to construct a PANoptosis signature with excellent efficacy
in recognising CD. PANoptosis may mediate the process of CD through inflammatory
and immune mechanisms, such as NF- kappaB, MAPK and leukocyte migration.
A Comprehensive Approach for Diabetic Foot Ulcer Management
Authors: Bakshi Anusha , Prajapati Bijal , Dharamsi Abhay , Sompura Krishna 
Published: October 2024

Objectives:
This study aims to evaluate the efficacy of a comprehensive management
plan in the treatment of diabetic foot ulcers (DFUs). Evaluating the effects of wound debridement
on the outcomes of diabetic foot ulcers, advanced dressings, blood sugar control, and offloading
strategies are the main objectives. The study also intends to investigate the function of surgery in
managing chronic ulcers and preventing recurrences. The goal is to offer advice for managing
DFUs optimally and lowering related morbidity based on evidence.

Method:
The intervention program includes techniques like wound debridement, advanced dressings,
unloading modalities, blood sugar level control, and, where necessary, surgical treatments.
Growth factors, electrical stimulation, negative pressure wound therapy, bio-engineered skin, and
hyperbaric oxygen therapy are among the many treatments that are included. Regression models
are among the statistical analyses used to assess the efficacy of the overall management strategy.

Results:
Significant findings are presented in the results section, which shows how the holistic management
strategy improves DFU outcomes. Improved wound healing rates, reduced infection incidents,
and enhanced patient-reported outcomes are highlighted. The results underscore the significance
of maintaining blood sugar levels, wound debridement, and incorporating various therapeutic
modalities in DFU management.

Conclusion:
This study concludes by recommending a thorough and interdisciplinary approach to
DFU management. Maintaining blood sugar levels, debridement of the wound, sophisticated dressings,
unloading techniques, and surgical procedures are among the effective strategies that help reduce
morbidity and prevent serious complications related to diabetic foot ulcers. Additional investigation
is necessary to enhance and broaden these results for ongoing progress in the management
of diabetic foot ulcers.
A Retrospective Observational Study of Adverse Drug Reactions (ADR) Reported to ADR Monitoring Centre from 2010 to 2020
Authors: Srinivasan Akila , Selvarajan Sandhiya , Shivabasappa Santhosh , Singaravelu Arunmozhy 
Published: October 2024

Background:
Adverse Drug Reactions (ADR) are one of the essential causes of hospital
admissions and pose a significant clinical and economic burden on the healthcare system.
The Adverse Drug Reaction Monitoring Centre (AMC) in JIPMER functioning under the Pharmacovigilance
Programme of India (PvPI) plays a vital role in ensuring medication safety by
routinely detecting and monitoring ADRs. Hence, this study aimed to assess the characteristics
of ADR reported from 2010 to 2020 in AMC JIPMER and to detect signals, if any. Objectives:
To study the characteristics of Adverse Drug Reactions (ADR) reported to a regional ADR monitoring
center from 2010 to 2020 and to detect signals of disproportionate reporting (SDRs) if
any from the reported ADRs.

Materials and Methods:
A total of 6007 ADR reports with a single suspect drug were included
for analysis from 2010 to 2020. The characteristics of these reports, including patient’s age and
gender, Number and percentage of ADRs, the causality of ADR using WHO UMC (World
Health Organization-Uppsala Monitoring Scale), the seriousness of the ADR, and outcome were
collected from the ADR reports. MedDRA (Medical Dictionary for Regulatory Activities) Preferred
Terms (PT) were used to classify adverse drug reactions. Causality analysis using the Naranjo
Algorithm and Preventability using Modified Schumock and Thornton criteria were performed
for the ADRs. The number and percentage of severe ADRs were analyzed. The System
Organ class of all the ADRs was enumerated. ADRs not mentioned in the US FDA (United
States Food and Drug Administration) product label (unlabelled reactions) were documented.
Unlabeled reactions with ≥3 ADR reports were included for signal detection by disproportionality
analysis.

Results:
Antineoplastic drugs, followed by antimicrobials, anticonvulsants, Anti snake venom,
and NSAID were the most common drugs implicated in ADRs. Skin and subcutaneous tissue
disorders were the most common System Organ Class (SOC) involved in the ADRs. Among the
6007 reports, 19.2% were serious ADRs. Most of the ADR reports were of possible causality
followed by probable and certain as per WHO UMC and Naranjo causality scales. Only ten
ADRs were preventable and one reaction (Tamoxifen-induced neuropathy) was eligible for signal
detection. Disproportionality analysis using a 2x2 contingency table showed insignificant
signal detection using the Reporting Odds Ratio (ROR) and Proportional Reporting Ratio
(PRR).

Conclusion:
Analysis of ADRs from an ADR Monitoring center functioning in a tertiary care
hospital shows antineoplastic drugs to be the most common drugs associated with adverse drug
reactions, with rash being the most common adverse effect. The majority of the ADRs were not
preventable. No Signals of Disproportionate Reporting (SDR) were detected in our study.
Promotion of Epithelial Healing in Oral Mucositis by hESC-Derived Mesenchymal Stem Cells via the PI3K/AKT Pathway
Authors: Lv Kejia , Gao Bicong , Ye Weijia , Shen Chenlu , Chen Tianchi , Wang Chaowei , Yao Hua 
Published: October 2024

Introduction:
Oral mucositis (OM) is a common and debilitating side effect of cancer
therapies such as radiotherapy, chemotherapy, hematopoietic cell transplant, or their combinations.

Method:
This study focused on the reparative effects of human embryonic stem cell-derived mesenchymal
stem cells (hESC-MSCs) in OM and possible mechanisms. An ulcer model was created
in the rat buccal mucosa to mimic an in vivo animal model of OM mucosal injury, and hESC-MSCs
were injected 48h later to assess their reparative effects. In vitro, the efficacy of hESC-MSCs
in regulating apoptosis and proliferation in LPS- or 5-fluorouracil (5-FU)-injured HaCaT cells
was studied using a transwell coculture system. Subsequently, the PI3K inhibitor LY24002 was
used to assess whether hESC-MSCs regulated injured HaCaT cells through the PI3K/AKT pathway.
In vivo, we found that hESC-MSCs injection promoted OM healing in rats through the acceleration
of re-epithelialization and a decrease in apoptosis.

Results:
In vitro, our findings revealed that the hESC-MSCs treatment led to a reduction in the
quantity of HaCaT cells undergoing apoptosis. Western blot analysis revealed that hESC-MSCs
activated AKT, resulting in increased protein levels of PCNA and BCL-2 and decreased protein
levels of Bax and Caspase-3 whereas LY294002 reversed these changes.

Conclusion:
These findings suggest that hESC-MSCs promoted OM wound healing by stimulating
the proliferation of epithelial cells and inhibiting their apoptosis in rat models. Furthermore,
hESC-MSCs might mediate the PI3K/AKT pathway to modulate apoptosis/proliferation injured
by LPS or 5-FU in HaCaT cells.
Thiazolidine-4-one Analogues: Synthesis, In-Silico Molecular Modeling, and In-vivo Estimation for Anticonvulsant Potential
Authors: Mittal Payal , Ghanghas Deepak , Sharma Diksha , Shah Kamal , Arya Girish Chandra , Chaudhary Aarti , Dewangan Hitesh Kumar 
Published: October 2024

Background:
Epilepsy is a critically deep-rooted CNS disorder affecting above 50 million
people all over the world. Thus, a safe and effective treatment that proves its worth in this ailment
is urgently needed. Thiazolidine-4-ones possess the molecules to be used as anticonvulsants.
The thiazolidinedione is a cyclic analogue of thiosemicarbazides and thioureas as well as a (bio)isostere
of hydantoin (imidazolidine-2,4-dione), which are recognized as novel anticonvulsant designs.

Aim:
This study aimed to develop and evaluate a novel thiazolidine-4-one derivative by three-component
condensation in one pot reaction method.

Methods:
A novel thiazolidine-4-one derivative was formulated by three-component condensation.
The selected OH (Alcohol) derivatives were found to be more potent; hence, a molecular
docking study against a selected target LGI1 LRR domain was performed. Various analytical tests
like FTIR and H1 NMR were accomplished. The FTIR was used to validate the existence of multiple
functional moieties like C-S, O-H, C=O, C-N, N=O, C-NH, C-O in the wave region from 3075
cm-1 – 1236 cm-1 and H1 NMR was employed to ascertain if the synthesized analogues had the
complete set of protons. Then, the anti-seizure activity of the selected compound was examined using
PTZ models in mice at three successive doses, i.e., 25, 50, and 100mg/kg, and compared with
standard ethosuximide.

Results:
The docking simulations were initiated using PyMOL after the binding site was determined
and the receptor and ligand were suitably prepared. It showed higher binding frequency in
comparison to the standard marketed drug Ethosuximide. FTIR and H1 NMR spectroscopy were
used to characterize the chemical components. Numerous functional groups, including O-H (alcohol),
C=O (ketones), N=O, C-NH, C-N, C-S, and C-O bending stretching, were visible in the synthesized
molecule accordingly. The synthesized compound was effective in inhibiting the convulsions
at the concentration of 100 mg/kg.

Conclusion:
The novel thiazolidine-4-one derivative showed promising activity and could be considered
for further investigation and dosage form preparation.