bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon induced transmembrane protein 3 regulates the growth and invasion of human lung adenocarcinoma
Authors: Zhang Dong , Wang Huimin , He Huijie , Niu Haiying , Li Yu 
Published: May 2017

BackgroundInterferon induced transmembrane protein 3 (IFITM3) plays an important role in the tumorigenesis and progression of multiple cancers. This study investigated the expression and function of IFITM3 in human lung adenocarcinoma.MethodsFifty human lung adenocarcinoma tissues were collected. IFITM3 expression was assessed by immunohistochemical staining. The clinicopathologic characteristics of all patients were analyzed.ResultsIFITM3 was mainly detected in the cytoplasm of advanced cancer tissues and its expression was correlated with tumor malignancy grade. Knockdown of IFITM3 in vitro markedly inhibited the proliferation and invasion of lung adenocarcinoma cells.ConclusionIFITM3 represents a potential therapeutic target for the treatment of lung adenocarcinoma.
Nursing Diagnosis Risk for Delayed Surgical Recovery (00246): Concept Clarification and Definition of Empirical Referents
Authors: Rembold Simone Martins , Santana Rosimere Ferreira , de Souza Priscilla Alfradique , Schwartz Sócrates Miranda de Oliveira Xavier 
Published: May 2017

PURPOSETo clarify the concept of risk for delayed surgical recovery (00246) and to define the empirical referents for identification of predictive factors of delay in recovery.METHODA concept analysis was developed based on the eight steps proposed by Walker and Avant.RESULTSDefining attributes were determined and antecedents and consequents identified. Cases were proposed cases and empirical referents established.CONCLUSIONThe results provided evidences and instrumentalize empirical referents, which may support an accurate nursing diagnosis.IMPLICATIONS FOR NURSING PRACTICEThis clarification may contribute to the return to daily activities in a shortest time and promote surgical safety of the patient.OBJETIVOSClarificar o conceito de risco de recuperação cirúrgica retardada (00246) e definir as referências empíricas para a identificação dos fatores preditores de atraso na recuperação.MÉTODOFoi desenvolvida a análise de conceito desse fenômeno, com base nas oito etapas propostas por Walker e Avant.RESULTADOSForam determinados os atributos definidores e identificados seus antecedentes e consequentes. Foram propostos casos e estabelecidas as referências empíricas.CONCLUSÕESOs resultados proporcionaram evidências e instrumentalização das referências empíricas, o que pode auxiliar na acurácia do diagnóstico de enfermagem.IMPLICAÇÕES PARA A PRÁTICA DE ENFERMAGEMEsta clarificação poderá contribuir para o retorno do paciente às atividades diárias em um tempo mais curto e promoção da segurança cirúrgica.
Norepinephrine reduces arterial compliance less than phenylephrine when treating general anesthesia‐induced arterial hypotension
Authors: Vallée F. , Passouant O. , Le Gall A. , Joachim J. , Mateo J. , Mebazaa A. , Gayat E. 
Published: May 2017

IntroductionDuring general anesthesia, arterial hypotension is frequent and may be an important contributor to perioperative morbidity. We assessed the effect of a 5 μg bolus of Norepinephrine (NA) when compared with 50 μg bolus of Phenylephrine (PE) administered to treat hypotension during maintenance anesthesia, on MAP, derived cardiac output and arterial stiffness parameters.MethodsPatients scheduled for a neurosurgical procedure under general anesthesia were prospectively included. Monitoring included invasive blood pressure, esophageal Doppler, and arterial tonometer used to estimate central aortic pressure with arterial stiffness parameters, such as augmentation index (Aix). After initial resuscitation, hypotensive episodes were corrected by a bolus administration of NA or PE in a peripheral venous line.ResultsThere were 269 bolus administrations of vasopressors (149 NA, 120 PE) in 47 patients with no adverse effects detected. A decrease in stroke volume (SV) was observed with PE compared with NA (−18 ± 9% vs. −14 ± 7%, P < 0.001). This decrease was associated with an increase in Aix, which was greater for PE than for NA (+10 ± 8% vs. +6 ± 6%, P < 0.0001), and a decrease in total arterial compliance greater for PE compared to NA (Ctot = SV/Central Pulse Pressure) (−35 ± 9% vs. −29 ± 10%, P < 0.001).DiscussionThis study suggests that 5 μg of NA administered as a bolus in a peripheral venous line could treat general anesthesia‐induced arterial hypotension with a smaller decrease in SV and arterial compliance when compared to PE.
Estimations of linkage disequilibrium, effective population size and ROH‐based inbreeding coefficients in Spanish Churra sheep using imputed high‐density SNP genotypes
Authors: Chitneedi P. K. , Arranz J. J. , Suarez‐Vega A. , García‐Gámez E. , Gutiérrez‐Gil B. 
Published: May 2017

SummaryIn this study, the availability of the Ovine HD SNP BeadChip (HD‐chip) and the development of an imputation strategy provided an opportunity to further investigate the extent of linkage disequilibrium (LD) at short distances in the genome of the Spanish Churra dairy sheep breed. A population of 1686 animals, including 16 rams and their half‐sib daughters, previously genotyped for the 50K‐chip, was imputed to the HD‐chip density based on a reference population of 335 individuals. After assessing the imputation accuracy for beagle v4.0 (0.922) and fimpute v2.2 (0.921) using a cross‐validation approach, the imputed HD‐chip genotypes obtained with beagle were used to update the estimates of LD and effective population size for the studied population. The imputed genotypes were also used to assess the degree of homozygosity by calculating runs of homozygosity and to obtain genomic‐based inbreeding coefficients. The updated LD estimations provided evidence that the extent of LD in Churra sheep is even shorter than that reported based on the 50K‐chip and is one of the shortest extents compared with other sheep breeds. Through different comparisons we have also assessed the impact of imputation on LD and effective population size estimates. The inbreeding coefficient, considering the total length of the run of homozygosity, showed an average estimate (0.0404) lower than the critical level. Overall, the improved accuracy of the updated LD estimates suggests that the HD‐chip, combined with an imputation strategy, offers a powerful tool that will increase the opportunities to identify genuine marker‐phenotype associations and to successfully implement genomic selection in Churra sheep.
Hypogonadism in testicular cancer patients is associated with risk factors of cardiovascular disease and the metabolic syndrome
Authors: Bogefors C. , Isaksson S. , Bobjer J. , Kitlinski M. , Leijonhufvud I. , Link K. , Giwercman A. 
Published: May 2017

SummaryMore than 95% of testicular cancer are cured but they are at increased long‐term risk of cardiovascular disease. The risk of cardiovascular disease and treatment intensity was reported, but it is unknown whether this effect of cancer therapy is direct or indirect, mediated through androgen deficiency. Our aim was, therefore, to evaluate whether testicular cancer patients have increased the prevalence of risk factors of cardiovascular disease and if these risk factors are associated with hypogonadism and/or the cancer treatment given. In 92 testicular cancer patients (mean 9.2 years follow‐up) and age‐matched controls, blood samples were analysed for lipids, total testosterone, luteinizing hormone (LH), glucose and insulin. An estimate of insulin resistance, HOMAir was calculated. Hypogonadism was defined as total testosterone < 10 nmol/L and/or LH > 10 IU/L and/or androgen replacement. In testicular cancer men with hypogonadism, compared with eugonadal patients, higher insulin (mean difference: 3.10 mIU/L; p = 0.002) and HOMAir (mean difference: 0.792; p = 0.007) were detected. Hypogonadism group presented with increased risk (OR = 4.4; p = 0.01) of metabolic syndrome. Most associations between the treatment given and the metabolic parameters became statistically non‐significant after adjustment for hypogonadism. In conclusion, testicular cancer patients with signs of hypogonadism presented with significantly increased risk of metabolic syndrome and investigation of endocrine and metabolic parameters is warranted in these patients.
Spermatozoa from males with reduced fecundity exhibit differential DNA methylation patterns
Authors: Laqqan M. , Tierling S. , Alkhaled Y. , Lo Porto C. , Solomayer E. F. , Hammadeh M. 
Published: May 2017

AbstractInfertility affects 10–15% of couples, and approximately 50% of cases are linked to male factor infertility. The purpose of this study was to evaluate the DNA methylation patterns in spermatozoa from males who are suffering from a reduction in fecundity. Thirty samples were subjected to 450K arrays as a screening study to evaluate the variation in sperm DNA methylation levels between cases and controls groups, and then four CpG sites (cg05799088, cg07227024, cg16338278, and cg08408433) underwent to deep bisulfite sequencing to validate the observed methylation differences in 111 samples (56 proven fertile males as ‘controls’ and 55 males suffering from a reduction in fecundity as ‘cases’). A significant difference in the mean methylation level was found between cases and controls in the CpGs of PRICKLE2 gene‐related amplicon (CpG1, p ≤ 0.002, and CpG2, p ≤ 0.004) and CpG of ALS2CR12 gene‐related amplicon (CpG1, p ≤ 0.015, and CpG2, p ≤ 0.009). Besides, a significant difference was found at seven from thirteen CpGs tested in the ALDH3B2 gene amplicon CpG2, CpG6, CpG9, CpG10, CpG11, CpG12, and CpG13 (p ≤ 0.005, p ≤ 0.004, p ≤ 0.012, p ≤ 0.028, p ≤ 0.012, p ≤ 0.009, and p ≤ 0.001, respectively). In addition, the results showed that nine CpGs out of the twenty‐six within the PTGIR gene‐related amplicon (CpG4, CpG6, CpG8, CpG9, CpG11, CpG15, CpG19, CpG23, and CpG26) had a significant difference in their mean methylation level (p ≤ 0.006, p ≤ 0.009, p ≤ 0.003, p ≤ 0.003, p ≤ 0.007, p ≤ 0.002, p ≤ 0.018, p ≤ 0.018, and p ≤ 0.040, respectively) in the case vs. control group. In conclusion, an alteration in the methylation levels of sperm DNA from males with reduced fecundity was observed. In addition, an association between changes in the methylation level for these CpGs and different semen parameters has been found.
Maintenance of the contractile phenotype in corpus cavernosum smooth muscle cells by Myocardin gene therapy ameliorates erectile dysfunction in bilateral cavernous nerve injury rats
Authors: Zhang H.‐B. , Wang Z.‐Q. , Chen F.‐Z. , Ding W. , Liu W.‐B. , Chen Z.‐R. , He S.‐H. , Wei A.‐Y. 
Published: May 2017

AbstractThe pathophysiology of erectile dysfunction post radical prostatectomy is not clearly clarified, and the low efficacy of traditional PDE5i treatment remains a major complaint in contemporary practice. This study aimed to demonstrate phenotypic modulation in bilateral cavernous nerve injury (BCNI) rats within 7 days, and subsequently validate gene therapy with Myocardin (Mycod) by maintaining a contractile phenotype in corpus cavernosum smooth muscle cells. Initially, 36 male rats were randomly divided into BCNI and negative control (NC) groups for histological and phenotypic molecular measurements at 3, 5, and 7 days. Afterwards, an additional 30 rats received a single intra‐cavernous injection of 50 μL PBS, Ad‐Myocd (1 × 1011 pfu/ml) or Ad‐vector for 10 animals each, namely the NC+PBS, BCNI+Ad‐Myocd, and BCNI+Ad‐vector groups. Finally, the validity and mechanism of Myocd transfection was explored at 21 days in vivo and 48 h in vitro. Western blotting showed canonical declines in Myocd, α‐SMA, and Calponin expression, as well as elevated Osteopontin (OPN) expression, before corporeal morphological and SM‐to‐collagen ratio changes at day 5 after injury. Overexpression of Myocd maintained the contractile phenotype of corpus cavernosum smooth muscle cells, ameliorated bilateral cavernous nerve injury rat erectile dysfunction, as well as promoted cell contractility and suppressed proliferative capacity. Simultaneously, confocal imaging revealed up‐regulation and co‐localization of serum response factor in gene‐transferred cells. In conclusion, our study is the first to investigate corpus cavernosum smooth muscle cells phenotypes in the early stages of cavernous injury model rats, and Myocd reversed phenotypic modulation by activating serum response factor. The experimental results demonstrated the validity of gene therapy for erectile dysfunction.
Predictive Factors Upon Discontinuation of Renal Replacement Therapy for Long‐Term Chronic Dialysis and Death in Acute Kidney Injury Patients
Authors: Yang Tingting , Sun Si , Lin Liping , Han Mei , Liu Qiang , Zeng Xiaoxi , Zhao Yuliang , Li Yupei , Su Baihai , Huang Songmin , Yang Lichuan 
Published: May 2017

AbstractThe specific timing for discontinuing renal replacement therapy (RRT) in acute kidney injury (AKI) patients is debatable. The predictive abilities of variables at the time of discontinuation of RRT for the long‐term prognoses of patients have not been explored. This study aimed to explore the prognostic factors upon discontinuation of RRT for long‐term chronic dialysis and death of patients with acute RRT‐requiring AKI, thus improving decision making regarding the discontinuation of RRT and the follow‐up of patients thereafter. A cohort of 302 AKI patients who required acute RRT and remained alive and free of dialysis for at least 30 days after discharge from January 2009 to December 2012 were followed up. The predictive abilities of general characteristics, RRT details, and variables upon discontinuation of RRT for long‐term chronic dialysis and all‐cause death were evaluated using Cox proportional hazards models. Kaplan–Meier analysis with a log‐rank test was used to compare the survival curves between the strata of levels of good predictors upon discontinuation of RRT. After a median follow‐up time of 4.1 years, 20 (6.6%) patients initiated chronic dialysis and 56 (18.5%) patients died. A higher CysC level upon discontinuation of RRT (HR 1.520, 95% CI 1.082–2.135; P = 0.016), comorbid chronic kidney disease, and a higher non‐renal Charlson comorbidity index (CCI) were independently predictive for chronic dialysis. The hemoglobin level upon discontinuation of RRT was inversely predictive of death (HR 0.986, 95% CI 0.973–0.999; P = 0.035), and comorbid malignancy, the presence of multiple organ dysfunction syndrome, and a higher non‐renal CCI also predicted death. Urine output upon discontinuation of RRT was marginally inversely predictive of death (HR 0.997, 95% CI 0.994–1.000; P = 0.056). Patients who discontinued RRT with CysC levels <2.97 mg/L, hemoglobin levels >85 g/L, and urine output >1130 mL/24 h showed significantly higher non‐chronic dialysis and survival rates according to a log‐rank test. Our study suggested that upon discontinuation of RRT, higher serum CysC levels had the most promising predictive value for long‐term chronic dialysis, and lower hemoglobin levels predicted long‐term death; lower urine output also marginally predicted long‐term death. Based on the remission of the comprehensive condition, lower CysC levels and higher hemoglobin levels and urine output should be considered in the decision to stop RRT. Patients showing worse levels of these indices upon discontinuation of RRT should undergo stricter follow‐up and treatment to improve long‐term outcomes.
The catechol‐O‐methyltransferase (COMT) Val158Met genotype modulates working memory‐related dorsolateral prefrontal response and performance in bipolar disorder
Authors: Miskowiak KW , Kjærstad HL , Støttrup MM , Svendsen AM , Demant KM , Hoeffding LK , Werge TM , Burdick KE , Domschke K , Carvalho AF , Vieta E , Vinberg M , Kessing LV , Siebner HR , Macoveanu J 
Published: May 2017

ObjectivesCognitive dysfunction affects a substantial proportion of patients with bipolar disorder (BD), and genetic‐imaging paradigms may aid in the elucidation of mechanisms implicated in this symptomatic domain. The Val allele of the functional Val158Met polymorphism of the catechol‐O‐methyltransferase (COMT) gene is associated with reduced prefrontal cortex dopamine and exaggerated working memory‐related prefrontal activity. This functional magnetic resonance imaging (fMRI) study investigated for the first time whether the COMT Val158Met genotype modulates prefrontal activity during spatial working memory in BD.MethodsSixty‐four outpatients with BD in full or partial remission were stratified according to COMT Val158Met genotype (ValVal [n=13], ValMet [n=34], and MetMet [n=17]). The patients completed a spatial n‐back working memory task during fMRI and the Cambridge Neuropsychological Test Automated Battery (CANTAB) Spatial Working Memory test outside the scanner.ResultsDuring high working memory load (2‐back vs 1‐back), Val homozygotes displayed decreased activity relative to ValMet individuals, with Met homozygotes displaying intermediate levels of activity in the right dorsolateral prefrontal cortex (dlPFC) (P=.016). Exploratory whole‐brain analysis revealed a bilateral decrease in working memory‐related dlPFC activity in the ValVal group vs the ValMet group which was not associated with differences in working memory performance during fMRI. Outside the MRI scanner, Val carriers performed worse in the CANTAB Spatial Working Memory task than Met homozygotes (P≤.006), with deficits being most pronounced in Val homozygotes.ConclusionsThe association between Val allelic load, dlPFC activity and WM impairment points to a putative role of aberrant PFC dopamine tonus in the cognitive impairments in BD.
Annotation: Developing a national programme for VTE prevention
Authors: Roberts Lara N. , Durkin Mike , Arya Roopen 
Published: May 2017

SummaryThe National Venous Thromboembolism Prevention Programme was launched in England, in 2010. Its central objective was to reduce morbidity and mortality from preventable deep vein thrombosis and pulmonary embolism through introduction of a comprehensive systematic approach. The cornerstone of the programme was the introduction of mandatory documented risk assessment for venous thromboembolism, supported by national thromboprophylaxis guidance. Despite widespread uptake of risk assessment, measuring the impact of the national programme on outcomes has proved challenging. The aim of this paper is to review the implementation and outcomes of the national programme.
Impact of body mass index on robot‐assisted radical cystectomy with intracorporeal urinary diversion
Authors: Ahmadi Nariman , Clifford Thomas G. , Miranda Gus , Cai Jie , Aron Monish , Desai Mihir M. , Gill Inderbir S. 
Published: May 2017

ObjectivesTo determine the impact of body mass index (BMI) on peri‐operative and oncological outcomes after robot‐assisted radical cystectomy (RARC) with intracorporeal urinary diversion.Patients and MethodsA total of 216 patients undergoing RARC, extended lymphadenectomy and intracorporeal urinary diversion, between July 2010 and December 2015, were categorized into four BMI groups according to the 2004 World Health Organization obesity classification groups: <25 kg/m2 (normal); 25–29.9 kg/m2 (pre‐obese); 30–34.9 kg/m2 (obese class I); and ≥35 kg/m2 (obese class II). Pre‐, intra‐ and postoperative characteristics, oncological outcomes, and 90‐day complications were compared using sas statistical software.ResultsAll 216 patients underwent intracorporeal urinary diversion, with 68 (32%) undergoing orthotopic neobladder construction. Demographics were similar among the BMI groups with regard to median (range) age (71.8 [35– 95] years), gender (80.6% men), Charlson comorbidity index (CCI) score (66.2% with CCI score 0–1), pathological stage (carcinoma in situ to T2: 55.1%, T3–T4/N0: 18.5%, Tx/N+: 26.4%), median (interquartile range) node count [41 (28, 53)] and positive soft tissue margin rate (4.2%). Obese patients had greater blood loss and longer operating time (P = 0.02 and P = 0.04, respectively). There were no significant differences in length of hospital stay, transfusion rates, readmission or 90‐day overall and high‐grade complication rates (P = 0.16, P = 0.96, P = 0.89, P = 0.22 and P = 0.51, respectively). At a median (range) follow‐up of 13 months (15 days to 4.8 years), recurrence‐free survival (P = 0.92) and overall survival (P = 0.68) were similar among the groups.ConclusionThe results of the present study show that RARC with intracorporeal urinary diversion is safe and feasible in obese patients with bladder cancer. BMI was not associated with significant differences in peri‐operative, pathological or early oncological outcomes.
Understanding the school experiences of children and adolescents with serious chronic illness: a systematic meta‐review
Authors: Lum A. , Wakefield C. E. , Donnan B. , Burns M. A. , Fardell J. E. , Marshall G. M. 
Published: May 2017

AbstractBackgroundSerious chronic illness can have a detrimental effect on school attendance, participation and engagement, leaving affected students at risk of failing to meet their developmental potential. An improved understanding of factors that help to explain or mitigate this risk can help educators and health professionals deliver the most effective support. This meta‐review critiqued the available evidence examining the link between six chronic illnesses (asthma, cancer, chronic kidney diseases, heart diseases, cystic fibrosis and gastrointestinal diseases) and children's and adolescents' school experiences and outcomes, as well as investigating the medical, school, psychosocial and sociodemographic factors that are linked to poorer or better school outcomes.MethodsWe searched CINAHL, Cochrane Database, EMBASE, ERIC, MEDLINE, ProQuest Theses and Dissertations, and PsycINFO (2000–2015). Systematic and narrative reviews, and meta‐analyses, of original studies examining students' subjective school experiences and objective school outcomes were eligible. We used the Preferred Reporting Items for Systematic Reviews and Meta‐analyses criteria to critically appraise all systematic reviews. The Grading of Recommendations Assessment, Development, and Evaluation system guided our recommendations for practice and research.ResultsEighteen reviews of 172 studies including more than 40 000 students were eligible. Therefore, we chose to conduct a meta‐review to provide an overview of the literature on the relationship between chronic illness and school experiences and outcomes. We also explored the associated medical, school, psychosocial and sociodemographic factors affecting the relationship between illness and school experiences and outcomes.ConclusionStudents with chronic illness demonstrate mixed school experiences and outcomes that are often worse than students without chronic illness. Modifiable factors, such as students' engagement with school, may be novel yet appropriate targets of educational support to ensure that these students reach their full schooling potential.