bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex Regional Pain Syndrome: a cross-sectional study of physical symptoms, disability, and psychological health in long term
Authors: Lyckegård Finn Ellen , Parinder Astrid , Nyman Erika , Dahlin Lars. B. 
Published: September 2024

Abstract

Introduction:
Knowledge about long-time residual symptoms, disabilities, and psychological health in complex regional pain syndrome (CRPS) is limited.

Objectives:
The aim was to evaluate outcome, focusing on physical symptoms, disability, and psychological health, in individuals with CRPS through a cross-sectional survey study.

Methods:
Individuals with a confirmed diagnosis of CRPS were identified through medical charts and sent validated survey forms (Disabilities of the Arm, Shoulder and Hand—Quick version, Specific Hand Surgery Questionnaire-8 questions, EuroQol 5 Dimensions 3 levels, Life Satisfaction Questionnaire-11, Hospital Anxiety and Depression Scale, Pain Catastrophizing Scale, and Sense of Coherence-29) and complementary questions.

Results:
Responders (response rate: 99/238, 42%; CRPS type 1: 72%; CRPS type 2: 28%; time since diagnosis median: 59 [34–94] months) reported remaining symptoms and disability (Disabilities of the Arm, Shoulder and Hand—Quick version score: 45 [20–70]) and more improvement in type 1 than in type 2. Only 9% of individuals with CRPS reported no residual pain or discomfort. Approximately 60% had problems in daily activities, 49% had sleeping problems, and 90% experienced moderate-extreme pain with 23% still on sick leave. The Hospital Anxiety and Depression Scale survey revealed significantly higher scores than a Swedish reference population. Individuals with a low Sense of Coherence and high pain catastrophizing had worse disability and were less satisfied with their lives and physical and psychological health. A lower level of education and more anxiety were associated with worsened disability over time.

Conclusion:
Individuals with CRPS suffer in the long term from pain, sleeping problems, and limitations in daily activities with occurrence of anxiety and depression, resulting in dissatisfaction with many aspects of their lives. A low Sense of Coherence and high pain catastrophizing are associated with a worse outcome. Biopsychosocial aspects should be addressed in clinical practice.
Performance of the New CKD-EPI Creatinine-and Cystatin C–based Glomerular Filtration Rate Estimation Equation in Living Kidney Donor Candidate
Authors: Kakuta Yoichi , Maegawa-Higa Yoko , Matsumura Soichi , Fukae Shota , Tanaka Ryo , Yonishi Hiroaki , Nakazawa Shigeaki , Yamanaka Kazuaki , Isaka Yoshitaka , Nonomura Norio 
Published: September 2024

Background.
Accurate preoperative evaluation of renal function in living kidney donor candidates (LKDCs) is crucial to prevent kidney failure after nephrectomy. We examined the performance of various estimated glomerular filtration rate (eGFR) equations, including the new chronic kidney disease epidemiology collaboration (CKD-EPI) equation in LKDCs.

Methods.
We analyzed 752 LKDCs who were assessed for measured GFR by inulin clearance as part of routine pretransplant examination from 2006 to 2020. CKD-EPI2012 from cystatin C (CKD-EPI12cys), CKD-EPI2021 from creatinine (CKD-EPI21cr), CKD-EPI21cr-cys, Japanese modified (JPN) eGFRcr, and JPN eGFRcys were compared in determining the suitability for LKDCs.

Results.
CKD-EPI12cys had the lowest absolute and relative biases, with higher P30 and P10, followed by JPN eGFRcys, CKD-EPI21cr, and CKD-EPI21cr-cys. The root mean square error was least for CKD-EPI12cys, then JPN eGFRcys, CKD-EPI21cr-cys, CKD-EPI21cr, and JPN eGFRcr. CKD-EPI21cr, CKD-EPI12cys, and CKD-EPI21cr-cys estimated GFR higher, whereas JPN eGFRcr estimated GFR lower. At the threshold of 90 mL/min/1.73 m2, CKD-EPI21cr had the highest percentage of misclassification at 37.37%, whereas JPN eGFRcr had the lowest percentage of misclassification at 6.91%. Using the age-adapted approach, JPN eGFRcr had the lowest percentage of misclassification into overestimation at 7.31%. All eGFR had >5.0%, and CKD-EPI21cr had the highest percentage of misclassification at 21.94%. Conversely, CKD-EPI21cr-cys had the lowest percentage of misclassification into underestimation at 3.19%, both at the threshold of 90 mL/min/1.73 m2 and the age-adapted approach. JPN eGFRcr had the highest percentage at 33.38% and 40.69%, respectively.

Conclusions.
In evaluating the renal function of Japanese LKDCs, the new CKD-EPI equation had a lower rate of underestimation but a relatively high rate of overestimation. New GFR estimation formulas are needed to be tailored to each ethnic group to enhance the accuracy and reliability of donor selection processes.
Decoding Cytokine Networks in Ulcerative Colitis to Identify Pathogenic Mechanisms and Therapeutic Targets
Authors: Olbei Marton , Hautefort Isabelle , Thomas John P. , Csabai Luca , Bohar Balazs , Ibraheim Hajir , Saifuddin Aamir , Modos Dezso , Powell Nick , Korcsmaros Tamas 
Published: September 2024

AbstractUlcerative colitis (UC) is a chronic inflammatory disorder of the gastrointestinal tract characterised by dysregulated cytokine signalling. Despite the advent of advanced therapies targeting cytokine signalling, treatment outcomes for UC patients remain suboptimal. Hence, there is a pressing need to better understand the complexity of cytokine regulation in UC by comprehensively mapping the interconnected cytokine signalling networks that are perturbed in UC patients. To address this, we undertook systems immunology modelling of single-cell transcriptomics data from colonic biopsies of treatment-naive and treatment-exposed UC patients to build complex cytokine signalling networks underpinned by putative cytokine–cytokine interactions. The generated cytokine networks effectively captured known physiologically relevant cytokine–cytokine interactions which we recapitulated in vitro in UC patient-derived colonic epithelial organoids. These networks revealed new aspects of UC pathogenesis, including a cytokine subnetwork that is unique to treatment-naive UC patients, the identification of highly rewired cytokines across UC disease states (IL22, TL1A, IL23A, and OSM), JAK paralogue-specific cytokine-cytokine interactions, and the positioning of TL1A as an important upstream regulator of TNF and IL23A as well as an attractive therapeutic target. Overall, these findings open up several avenues for guiding future cytokine-targeting therapeutic approaches in UC, and the presented methodology can be readily applied to gain similar insights into other immune-mediated inflammatory diseases (IMIDs).One Sentence SummaryA systems immunology map of cytokine interaction networks in ulcerative colitis reveals novel insights into disease pathogenesis, with potential to guide future cytokine-targeting therapeutic strategies.
Elucidating early intestinal stem cell response to bacterial infection
Authors: Lebon S. , Davidzohn N. , Habshush Menachem A. , Katz A. , Wigoda N. , Rotkopf R. , Dadosh T. , Levin-Zaidman S. , Dezorella N. , Blumberger N. , Hoffman D. , Hofree M. , Biton M. 
Published: September 2024

AbstractIntestinal stem cells (ISCs) are the regenerative force of the gut epithelium. Lgr5+-ISC have been shown to respond to changes in their microenvironment by coping with different metabolites, adapting to caloric changes, and recovering from injury and inflammation. However, how pathogenic bacteria affect adult stem cell regeneration and, as a consequence, the overall tissue adaption to infection has yet to be explored in depth. Here, we interrogated early Lgr5+ ISC responses to an enteric intracellular pathogen by profiling individual IECs from the mouse small intestine. Utilizing GFP-labeledSalmonella enterica,we isolated intracellular invaded cells to elucidate invasion programs of epithelial cell subsets. In particular, we identified aSalmonella-specific infection signature comprised of antimicrobial peptide (AMP) genes, including the Defensin gene family. Our findings demonstrate thatSalmonella entericatargets differentiated Paneth, enterocytes, and stem/progenitor cells at these early stages of infection. In response, a rapid Lg5+ ISC-driven cellular remodeling to enterocyte and Paneth lineages expressing AMP genes is initiated to combat the intruders. Importantly, we uncovered an ISC differentiation program via inflammasome activation to protect the crypt environment, while eliminating infected stem cells from the overall stem cell pool. This novel Lgr5+ stem cell defense mechanism not only protects the gut epithelium from persistent bacterial infection but also promotes tissue regeneration. We propose epithelial remodeling to AMP-secreting cells as a novel innate immune response to handle different gut stresses mediated by Lgr5+ ISCs to maintain organizational principles of gut homeostasis and physiology.
Network-driven identification of indisulam neo-substrates for targeted protein degradation
Authors: Jarnuczak Andrew F. , Andres Angelo , Yogev Orli , Ashenden Stephanie K. , Ye Cheng , Pachl Fiona , Zhang Andrew , Cuomo Maria Emanuela , Jin Meizhong 
Published: September 2024

AbstractIndisulam, a DCAF15-based molecular glue degrader, induces widespread proteome changes with implications for cell division and chromosome segregation. While RBM39 and RBM23 are two well-characterized indisulam neo-substrates, additional targets likely exist. To identify those degradation targets, we applied a network-based approach to prioritize novel neo-substrates from large-scale omics data. Our approach integrates proteome-wide expression measurements with information from publicly accessible databases into a multiplex heterogeneous network. Utilizing a Random Walk with Restart algorithm, we identified a preliminary list of 30 neo-substrates. These proteins are likely interactors with DCAF15 in the presence of indisulam and are subject to subsequent degradation. Experimental validation of RBM5, one of the shortlisted candidates, confirmed its degradation in a proteasome-dependent manner, supporting its identification as a novel indisulam neo-substrate. Our work employs established network resources and analytical methods to effectively identify direct targets of indisulam molecular glue degrader. This approach is readily adaptable for exploring novel targets across other molecular glue systems, enhancing its applicability and value to the drug discovery community.SummaryMolecular glue neo-substrate identification via network analysis and proteomics profiling
PTPN11Mutation Clonal Hierarchy in Acute Myeloid Leukemia
Authors: Fobare Sydney , Sharpe Chia , Quinn Kate , Bryant Kinsey , Miles Linde A. , Bowman Robert L. , Cheney Carolyn , Furby Casie , Long Marissa , Fyock Kaytlynn , Wronowski Ben , Lerma James R. , Mullaney Allison , Mrózek Krzysztof , Nicolet Deedra , Sesterhenn Tom , Johnstone Megan E. , Rai Shesh N. , Pasare Chandrashekhar , Zimmermann Nives , Carroll Andrew J. , Stone Richard M. , Wang Eunice S. , Kolitz Jonathan E. , Powell Bayard L. , Perentesis John P. , Eisfeld Ann-Kathrin , Hertlein Erin , Byrd John C. 
Published: September 2024

SummaryMutations in protein tyrosine phosphatase non-receptor type 11 (PTPN11) have been considered late acquired mutations in acute myeloid leukemia (AML) development. To interrogate the ontogeny ofPTPN11mutations, we utilized single-cell DNA sequencing and identified thatPTPN11mutations can occur as initiating events in some AML patients when accompanied by strong oncogenic drivers, commonlyNPM1mutations. The co-driver role ofPTPN11mutations was confirmed in a novel murine model that exhibits an AML phenotype with early expansion of a diverse set of variably differentiated myeloid cells that engrafted into immunodeficient and immunocompetent mice. This immune diversity was reconstituted from early precursor cells when engrafted into immunodeficient mice. Moreover, immune diversity was also observed in the blast component of patient samples withNPM1andPTPN11mutations, providing novel antigen targets for immune based approaches in this subset of AML that is resistant to multiple targeted therapies.
Safeguarding economic stability: the interplay of fiscal dominance and monetary policy in South Africa
Authors: Buthelezi Eugene Msizi 
Published: September 2024

PurposeThe purpose of this study is to investigate the interplay between fiscal dominance and monetary policy in South Africa from 1960 to 2023.Design/methodology/approachThe study employs a structural vector autoregression (SVAR) medel to analyze the relationship between fiscal dominance and monetary policy. Short-term and long-term shocks of government borrowing and deficits are examined to understand their impact on inflation dynamics.FindingsFiscal dominance has a significant effect both in the short and long run. There is evidence that government debt and deficits increase inflation, overriding the effects of monetary policy aimed at maintaining price stability. On the other hand, the study reveals that money supply shocks have a greater effect in reducing fiscal dominance compared to interest rate shocks. The variance movement on inflation is significantly explained by government debt and deficits. This emphasizes the persistence of inflationary pressures associated with fiscal dominance, highlighting the importance of effective policy interventions to mitigate inflationary risks.Originality/valueThis study contributes to the existing literature by providing insights into the dynamics of fiscal dominance in South Africa. Moreover, this study extends the theoretical framework of the fiscal theory of the price level (FTPL) and the government budget constraint. This study contributes valuable insights into the dynamics of fiscal dominance in South Africa and offers guidance for policymakers in formulating strategies to safeguard economic stability.
A systematic review of stakeholder’s interest towards achieving net zero carbon building
Authors: Falana Justina , Osei-Kyei Robert , Tam Vivian W.Y. 
Published: September 2024

PurposeStakeholder interests are complex, sensitive and highly uncertain and may influence the development of net zero carbon building (NZCB). However, this study aims to conduct a systematic literature review to explore the stakeholder interests towards achieving NZCB.Design/methodology/approachA total of 62 articles were identified from the Scopus database and thoroughly reviewed to extract relevant information on stakeholders' interest towards achieving NZCB.FindingsA total of 28 stakeholder interests influencing the development of NZCB were identified from the literature and were classified into six major groups according to their uniqueness (economic, social, environmental, technological, political, regulatory and legal).Research limitations/implicationsThe findings of this study provide insight into the specific stakeholder interests towards achieving NZCB. Thus, the findings of this study could serve as a guide for future research, policy formulation and implementation to expedite the practice of building towards net zero carbon (NZC). Empirical studies are suggested in future studies to test and consolidate the theoretical claims of this study.Originality/valueThis paper undertakes a comprehensive systematic review of studies on stakeholder interests towards achieving NZCB, which is the least investigated in the literature.
The determinants of participation in online business forums: exploring the interplay of need factors and reciprocity
Authors: Gharib Rebwar Kamal 
Published: September 2024

PurposeThe purpose of this study is to enhance the understanding of the drivers of participation in online business forums (OBFs). Specifically, it examines how reciprocity and three distinct types of needs (functional, psychological and hedonic) shape the participation behaviour of members in OBFs.Design/methodology/approachThis study employs a multilevel analysis, integrating Social Exchange Theory and Uses and Gratification Theory to develop and validate a research framework. Data were collected via online questionnaires (N = 596) from 48 business forums on LinkedIn. The data analysis was carried out using PLS-SEM in stages. A confirmatory factor analysis was carried out to assess the measurement model, including validating the reliability and validity of the measurement items. The direct hypotheses were tested, followed by a post ad-hoc analysis to test the mediation and moderation hypotheses.FindingsThis study shows the impact of three need factors – functional, psychological and hedonic – and reciprocity on community members' participation behaviour in OBFs. The findings indicate that fulfiling functional, psychological and hedonic needs are key determinants driving active participation. The study further highlights the coexistence of two types of reciprocity: direct and indirect. However, the data analysis results show that only indirect reciprocity motivates participation in OBFs. Furthermore, the study reveals that indirect reciprocity not only precedes participation but also acts as a pivotal factor interacting with the interrelationship between the need factors and participation levels in OBFs.Originality/valueThis research advances theoretical understanding of participation in OBFs by offering novel insights into its complex, multi-faceted nature. Unlike some previous simplistic models, this study employs innovative multilevel analysis, uniquely demonstrating the synergistic impact of need factors and reciprocity on participation. It offers a nuanced perspective previously unexplored by addressing reciprocity’s paradoxical role and uncovering interconnections between various factors. This approach provides groundbreaking insights into OBF dynamics, advancing theoretical understanding while offering actionable strategies for enhancing member engagement and community development.
Harran earthen houses as a precedent for environmental comfort
Authors: Gülmüş Serhat , Alaçam Sema , Güzelci Orkan Zeynel 
Published: September 2024

PurposeThis study aims to conduct environmental comfort analyses of vernacular architecture to establish design principles for a more sustainable design domain. In the scope of this research, 47 individual Harran earthen houses, comprising 32 different types and six typologies are examined.Design/methodology/approachEnvironmental comfort is selected as an umbrella term for the analysis of thermal comfort, visual comfort, and natural ventilation performance criteria. The performance simulations are conducted utilizing ClimateStudio and SolidWorks software. These simulations yield values for thermal comfort, glare, daylight, solar radiation, airflow, and pressure, which are used to compare different Harran earthen house typologies.FindingsThe study’s results indicate that various environmental comfort standards are met by Harran earthen houses through passive systems, without the need for technology or mechanical equipment. In terms of thermal comfort, visual comfort, and natural ventilation performance criteria, a typology that has advantages in one criterion may have disadvantages in the others. Factors such as orientation, material selection, opening arrangement, and architectural form are found to have an impact on environmental comfort.Originality/valueThis study differs from previous Harran earthen house and environmental comfort studies by focusing on multiple performance criteria and conducting a typology-based comparison based on performance analysis. The results of the study are expected to provide valuable insights into the environmental comfort studies of Harran earthen houses, emphasizing their relevance and applicability in contemporary architectural and urban design.
Authoritarian cue effect of state repression
Authors: Zhu Jiangnan , Bai Steve , Kang Siqin , Wang Juan , Liu Kaixiao 
Published: September 2024

AbstractState repression in autocracies has long been assumed to elicit explicit or implicit disapproval from citizens. Recent studies suggest that authoritarian governments can garner support for repressive policies through active information manipulation or exploiting social cleavages. However, is it possible for citizens to support repression even without government manipulation? We propose the “authoritarian cue effect,” arguing that citizens’ attitudes toward state repression can be endogenously shaped by instances of state repression, which may be interpreted as cueing messages signaling the regime's disapproval of the punished behaviors. Using a novel belief correction survey experiment, we empirically demonstrate that state repression can induce the public to pick up on cues and automatically adopt the state's stance, perceiving repressed behavior as having more negative externalities and supporting state repression more. This cue effect suggests that authoritarian state repression can self‐legitimize and evade public opinion backlash in a less costly manner than previously presumed.
Utility of exome sequencing in primary spermatogenic disorders: From research to diagnostics
Authors: Riera‐Escamilla Antoni , Nagirnaja Liina 
Published: September 2024

AbstractBackgroundPrimary spermatogenic disorders represent a severe form of male infertility whereby sperm production is impaired due to testicular dysfunction, leading to reduced quality or quantity of spermatozoa. Gene‐centered research has certainly demonstrated the importance of the genetic factor in the etiology of both poor sperm morphology or motility and reduced sperm count. In the last decade, next‐generation sequencing has expanded the research to whole exome which has transformed our understanding of male infertility genetics, but uncertainty persists in its diagnostic yield, especially in large unrelated populations.ObjectiveTo evaluate the utility of exome sequencing in detecting genetic factors contributing to various traits of primary spermatogenic disorders, which is a crucial step before interpreting the diagnostic yield of the platform.Materials and MethodsWe manually curated 415 manuscripts and included 19 research studies that predominantly performed whole exome sequencing in cohorts of unrelated cases with primary spermatogenic defects.ResultsThe detection rate, defined as the fraction of cases with an identifiable genetic cause, typically remained below 25% for quantitative defects of spermatozoa, whereas improved rates were observed for traits of abnormal sperm morphology/motility and in populations enriched with consanguineous families. Unlike the quantitative defects, the genetic architecture of the qualitative issues of spermatozoa featured a small number of recurrent genes describing a large fraction of studied cases. These observations were also in line with the lower biological complexity of the pathways affected by the reported genes.Discussion and ConclusionsThis review demonstrates the variability in detection rates of exome sequencing across semen phenotypes, which may have an impact on the expectations of the diagnostic yield in the clinical setting.