bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Effects of Winter Irrigation during Seasonal Freezing-Thawing Period on Soil Microbial Ecological Properties
Authors: Yang YueFa , WANG Chunxia , HE Xinlin , WANG Hongxin , WANG Yuan , ZHOU Fangyuan , QIN Da , FAN Zikang 
Published: October 2024

Abstract

Background and aims
The seasonal freeze-thaw period is a critical time for restoring soil fertility. Winter irrigation during this period alters soil moisture in agricultural fields, which in turn affects the soil's temperature, nutrients, and microbial factors within the soil micro-environment. Soil microorganisms play a crucial role in soil nitrogen fixation. Investigating the changes in soil microbial communities during the seasonal freeze-thaw period under winter irrigation conditions can help further understand the regulatory mechanisms of winter irrigation on the soil microenvironment.The experimental micro-areas were selected at the Irrigation Experiment Station of Shihezi University. Methods Based on soil moisture conditions, winter irrigation quotas of 0, 45, 90, and 135 mm were established. Soil samples were collected according to the seasonal freeze-thaw process in the experimental area, and measurements were taken for soil moisture, temperature, salinity, nutrients, as well as the quantity and diversity indices of soil bacteria and fungi. Using single-factor correlation and principal component analysis, the interactions between soil microbial indicators and their influencing factors under winter irrigation conditions were examined.
Results
The results indicate that winter irrigation during the seasonal freeze-thaw period not only alters the composition and metabolic capacity of microbial communities but also affects the interrelationships among microbial diversity indices, thereby impacting microbial stability.
Conclusions
Furthermore, a winter irrigation quota of 45 mm during the seasonal freeze-thaw period has multiple positive effects on the soil micro-environment, effectively promoting plant growth, improving soil health, and supporting the development of sustainable agriculture.
Integration of metabolomics and transcriptomics analyses reveals the effects of nano-selenium on pak choi
Authors: WANG Yanyan , SUN Peiheng , NIE Mingying , ZHAN Jianyun , HUANG Liu , WU Junda , HE Xiaowu , LI Na , HU Longsong , LIU Shiyu , YUAN Chengfu , ZHOU Changming , CHEN Guangjie , HUANG Jialong , LI Xiaofei 
Published: October 2024

Abstract

Selenium is an essential nutrient element for plants, and optimal selenium levels can enhance plant growth and metabolism while improving yield and quality. Suzhouqing serves as a significant source of selenium for human consumption. However, the effects of selenium-enriched culture and the underlying mechanisms of selenium accumulation in it remain inadequately understood. This study investigated the impact of varying concentrations of nano-selenium (0, 5, 10 and 20 mg L− 1) on the growth and nutritional quality of Suzhouqing. The findings revealed that a low concentration (5 mg L− 1) of nano-selenium significantly increased leaf weight and total selenium content while modulating primary metabolites (soluble amino acids, proteins, sugars and ascorbic acid) as well as secondary metabolites (glucosinolates, phenolic acids and flavonoids), thereby enhancing both growth performance and nutritional quality. Furthermore, integrated metabolomics and transcriptomic analyses elucidated pathways involved in selenocompound metabolism, phenylpropanoid biosynthesis and flavonoid biosynthesis, including methionine S-methyltransferase, 5-methyltetrahydrofolate-homocysteine methyltransferase, kynurenine-oxoglutarate transaminase, thioredoxin reductase, phenylalanine ammonian-lyase, 4-coumarate-CoA ligase, flavonoid 3', 5'-hydroxylase, naringenin 3-dioxygenase, flavonol synthase and bifunctional dihydroflavonol 4-reductase. These results elucidate the physiological and molecular mechanisms through which nano-selenium influences plant growth and nutritional quality, thus providing theoretical basis and technical support for breeding and cultivation of selenium-rich pak choi.
The sensory and affective dimensions of neuropathic pain engage distinct interneuron plasticity patterns and separate circuitries in the medial prefrontal cortex.
Authors: Kuner Rohini , Gan Zheng , Oswald Manfred , Naser Paul , Tan Linette , Beretta Carlo , Wang Lirong , Kaushalya Sanjeev , Sonntag Ivo , Zheng Hongwei , Kuner Thomas 
Published: October 2024

Abstract

Deactivation of the medial prefrontal cortex, a key center for cognition and motivational actions, is a common feature of several chronic pain conditions. However, which precise circuits underlie deactivation and altered output in chronic pain in vivo is not adequately understood. Here, unbiased longitudinal multiphoton imaging of GABAergic interneuron activity in the rodent prelimbic cortex revealed sensitization in all classes of GABAergic interneurons and a phenotypic switch from unimodality to polymodality after nerve injury. Targeted circuit interrogation revealed that distinct interneuron classes subserve different functions in fostering neuropathic pain and uncovered novel functions for the thus far unstudied caudal geminal eminence-derived interneurons. Remarkably, the sensory and emotional components of neuropathic pain engaged separate prelimbic output circuits comprising distinct types of excitatory neurons and projections. These results explain how the medial prefrontal cortex guides motivated actions to avoid or overcome pain and how loss thereof upon prefrontal deactivation exacerbates neuropathic pain.
Type 2 Diabetes Prediction Method Based onDual-Teacher Knowledge Distillation and FeatureEnhancement
Authors: Zhao Jian , Gao Hanlin , Sun Lei , Shi Lijuan , Kuang Zhejun , Wang Haiyan 
Published: October 2024

Abstract

Diabetes prediction is a critical issue in healthcare, and early identification is particularly important as the number of peopleaffected by the disease continues to increase. Many patients fail to recognize their health problems in time, leading to latediagnosis, which in turn causes a large number of health problems and deaths every year. Therefore, it is crucial to developeffective methods for early diagnosis of diabetes.This study proposes a data preprocessing method that includes outlierremoval, missing value filling, and sparse autoencoder (SAE) feature enhancement. We propose a Dual-CNN Teacher-StudentDistillation Model (DCTSD-Model) for type 2 diabetes classification, aiming to improve the accuracy and reliability of prediction.SAE is used to expand the variables of the original data and enhance the feature expression ability. The proposed CNN andDCTSD-Model models are evaluated using 10-fold cross validation on the feature-enhanced dataset.The results show that afterusing SAE feature enhancement, DCTSD-Model generates soft labels through knowledge distillation of the dual teacher model,helps the student model learn rich category information, and solves the category imbalance problem through the weightedrandom sampler. Experiments show that the accuracy of DCTSD-Model in classification tasks reaches 98.57%.This model issignificantly better than other models in terms of classification performance and reliability, providing an effective solution fordiabetes prediction and laying a solid foundation for future research and applications.
FABP4 as a critical mediator in osteoporosis: inhibition strategies and therapeutic potential
Authors: Xie Qian , Du Xiangfu , Liang Jianhui , Shen Yanni , Ling Yufan , Huang Zhengji , Ke Zekai , Li Tai , Song Bing , Wu Tailin , Wang Yan , Tao Hui-Ren 
Published: October 2024

Abstract

Fatty acid-binding protein 4 (FABP4), a key lipid protein in metabolism and inflammation, has been suggested to be linked to osteoporosis (OP), though direct evidence is scarce. Here, we present the first clear evidence of FABP4's significant role in OP, supported by clinical data and comprehensive in vivo and in vitro experiments. Elevated serum FABP4 in OP patients inversely correlates with bone mineral density (BMD), with similar trends observed in OVX mice. While FABP4 does not influence osteoblast differentiation, it promotes osteoclast formation and bone resorption. The FABP4 inhibitor BMS309403, with an IC50 of 0.89 µM, inhibits osteoclast differentiation by modulating calcium ions and suppressing the Ca2+-Calcineurin-NFATc pathway. Oral BMS309403 increased BMD in OVX mice, albeit less effectively than alendronate, whereas bone-targeted PLGA nanoparticles showed comparable efficacy to alendronate. This research identifies FABP4 as a promising therapeutic target for OP, with significant clinical implications.
Ecosystem Productivity and Carbon Dynamics in Keibul Lamjao National Park, Manipur, India: A Grey Relational Analysis Perspective
Authors: Meetei Kambam Boxen , Tsopoe Meribeni , Chandra Girish , Mukhopadhyay Debaaditya , Giri Krishna 
Published: October 2024

Abstract

An in-depth understanding of carbon dynamics and ecosystem productivity is essential for conservation and management of different ecosystems. Ecosystem dynamics and carbon budget are assessed by estimating Net Ecosystem Production (NEP) across different global ecosystems. An ecological productivity assessment of forest and floating meadow ecosystems in Keibul Lamjao National Park (KLNP), Manipur, North East India was conducted using the multi-criteria decision-making process namely, Grey Relational Analysis (GRA). The analysis was performed on 24 selected criterions classified either as "higher-the-better" or "lower-the-better" based on their degree of influence on the carbon budget. Floating meadows exhibited a higher production of aboveground and belowground biomass and a higher total mortality and decay. Furthermore, the study found that floating meadows exhibited a higher soil organic carbon (SOC) and net soil organic matter (SOM) than the forest ecosystem. The forest ecosystem showed higher total respiration (RT), heterotrophic respiration (RH), and autotrophic respiration (RA) than floating meadows. Floating meadows exhibited a higher net primary productivity (NPP) of 616.49 ± 33.87 gCm⁻²yr⁻¹ than the forest ecosystem, which has a NPP of 566.64 ± 65.26 gCm⁻²yr⁻¹. Similarly, Floating meadows have higher NEP (495.25 ± 36.46 gCm−²yr⁻¹) than forest ecosystems (418.39 ± 65.76 gCm−²yr⁻¹). These characteristics have a significant influence on the carbon budget in floating meadows as compared to forest ecosystems, as shown by larger values of Grey Relational Coefficient (GRC) in GRA. The Floating Meadows Ecosystem (0.82) obtained 54.72% percentage gain in GRG value with the forest ecosystem (0.53). This study might help in improving KLNP and other adjutant areas for conservation and management policies from the vital information given on the importance of wetlands in carbon dynamics and ecosystem productivity.
Effect of Dietary Treatment on Growth and plasma IGF-1 and IGFBP-3 Levels of Children with Familial Hypercholesterolemia
Authors: Kartal Tuğçe , Köksal Gülden , Bulut Fatma Derya , Kor Deniz , Mungan Neslihan Önenli 
Published: October 2024

Abstract

Background/Objective: This study aims to determine the effects of dietary treatment on growth and the biochemical markers of growth in children with primary familial hypercholesterolemia.
Subjects/Methods: The study was conducted with 30 children aged 5-10 years diagnosed with familial hypercholesterolemia at Cukurova University Hospital. Sociodemographic characteristics and dietary habits of patients were queried, and anthropometric measurements were taken. Specific dietary treatments were arranged, detailed dietary education was provided, and monthly follow-ups were conducted. At the beginning and in the end of the study, 3-days (24-hour) food consumption records were collected, and biochemical parameters were recorded. The International Physical Activity Questionnaire-Short Form (IPAQ-Short Form) was used to assess the general physical activity levels of the patients.
Results: It was found that 53.3% of the patients were female, and 46.7% were male. The average age of patients was 92.93±19.89 months. A moderate negative relationship was observed between the 6th month IGFBP-3 Z-scores and BMI Z-scores (r=-0.460). A moderate negative relationship was found between the initial IGF-1 Z-scores and the percentage of consumed dietary carbohydrates (r=-0.417). When the change in protein intake was examined, a moderate positive relationship was observed between Delta (∆) total protein and Delta (∆) animal protein, Delta (∆) plant protein, and Delta (∆) IGF-1 values (r=0.693; r=0.392; r=0.356, respectively).
Conclusion: To ensure adequate growth in children diagnosed with primary familial hypercholesterolemia, the amount and the content of protein together with the percentage of daily carbohydrate consumption should be considered in dietary treatment planning. Compliance with the diet should be closely monitored through food consumption records, and adherence to physical activity recommendations should be encouraged.
SnO2/SnS2 heterojunction with mesoporous structure for improved photocatalytic degradation of sulfonamide antibiotics
Authors: Li Zhanyu , Li Pengyu , Fang Yike , Chen bingxu , He Danfeng , Sun Wei , Li Guohui , Sun Yuanyuan 
Published: October 2024

Abstract

The SnO2/SnS2 composite photocatalyst with mesoporous structure and S-type heterojunction was successfully constructed, and their photocatalytic degradation activity under visible light irradiation was investigated using sulfonamide antibiotics as the target degradants. The results show that SnO2/SnS2 obtained by hydrothermal reaction at 180°C presents a flower ball with a size of about 5μm, a relatively high specific surface area of 50 m2g-1 and a large mesoporous pore size of 12.33 nm, and has lower fluorescence intensity, stronger stability and more abundant active sites. It is worth noting that SnO2/SnS2 has a strong light response at the wavelength of 200-800nm, and degradation rate of SnO2/SnS2 was 1.7 times that of SnO2 and 1.5 times that of SnS2, after photocatalytic reaction time of 210 min, respectively. It is benefitting from that the S-type heterojunction structure formed by the coupling of mesoporous SnO2 and SnS2 has a synergistic effect, which reduces the band gap and improves the photocatalytic activity. It can be seen that the photocatalytic degradation of sulfonamides by SnO2/SnS2 belongs to the zero-order reaction, and the reaction rate constant increases with the increase of temperature.
Effects of temperature and nutrition stress on Anopheles stephensi, an Indian urban malaria vector
Authors: Ghosh Chaitali , Kumar Naveen , Mogaveerthi Soumya , Lamba Sanjay , Ramanjini Chethan Kumar , Joshi Soumya Gopal , Chandran Priyanka , Kumar Sampath , Swain Sunita 
Published: October 2024

Abstract

Background: Changing climate and complexity in ecological landscape can potentially expand the geographic distribution of mosquitoes (Diptera: Culicidae) to adapt and transmit various vector-borne diseases like malaria globally. Anopheles stephensi is a potential urban malaria vector in the Indian subcontinent. Temperature and nutrients are the important environmental stressors, which influence the life cycle and vector competence of mosquitoes.
Methods: Three experimental setups were designed in the laboratory by exposing An. stephensi to low-temperature (LT, 4°Cand 18±1°C), high-temperature (HT, 35.5±1°C) and nutrition-deficient (NT, 33 mg) conditions. Stress was induced to both juvenile and adult stages for eight consecutive generations. Data of life-stage parameters were recorded. Additionally, LT, HT and NT strains were infected with in vitro culture of Plasmodium falciparum to compare their vectorial competence. Cytological analysis using the ovarian polytene chromosomes of the three stressed strains was carried out to check the structural differences in chromosomal arms due to extreme stress. Through predictive mathematical modelling using linear regression analysis, we estimated the number of generations required for the stressed lines to become comparable to the control lines.
Results: Fecundity, egg–wing sizes, egg-to-adult developmental time, pupal weight, male-to-female ratio, and longevity of adults were decreased in HT and NT lines, whereas these parameters were increased in LT lines. The HT and NT lines developed faster from egg to adult emergence (7.5 and 11 days), whereas the duration was longer (25 days) in the LT line. The oocyst infection rates in in-vitro Plasmodium falciparum were higher in HT, LT, and NT lines than the control line. The NT line revealed paracentric inversions on the 3L polytene chromosome arm in 2 locations. Further, the predictive model suggested faster adaptation of the LT line than HT and NT lines.
Conclusion: Being an invasive species and responsible for urban malaria transmission, the present multi-transgenerational laboratory-based study provides novel insights into the effects of stress on the life cycle, vectorial competence, cytology and rate of adaptation of An. stephensi. The findings aid in assessing disease risk and developing suitable intervention measures.
How Childhood Experiences in Nature Might Influence Adulthood Participation in Nature-Based Recreation
Authors: Azhar Nurul Farahiyyah Binti , Numata Shinya , Ngo Kang Min , Hosaka Tetsuro 
Published: October 2024

Abstract

Participation in activities that occur in natural settings during childhood tends to influence the frequency with which an individual engages in nature-based recreation during adulthood, along with their perceptions of the natural environment in adulthood. This study examined how childhood nature experience (CNE) might affect nature-based recreational behavior among adults residing in urban settings. We conducted a questionnaire survey of 1,004 urban residents in the Singapore city-state and found that urban parks were among the most frequently visited outdoor spaces, whereas more natural green spaces were less frequently visited. We found that CNE had a significant and robust positive effect on nature-based activities (NBA); this effect was followed by the effects of gender and income. Our findings to reveal the most frequently visited outdoor space among Singaporean and the correlation between the frequencies of CNE and sociodemographic have different effect on NBA adulthood. Thus, our findings suggest that CNE is strongly linked to adult NBA and is common among adults, particularly in the Asian region. Our findings may be utilized to inform the design and management of outdoor areas for younger generations, with the aim of encouraging the development of early childhood outdoor exposure.
Transcriptomics of acute injured lungs reveals IGF1R action on DNA damage response, metabolic reprogramming, mitochondrial homeostasis and epigenetics
Authors: Urtubia Alfredo , Piñeiro-Hermida Sergio , Alfaro-Arnedo Elvira , Canalejo Marta , de Toro María , Pichel José G. , López Icíar P. 
Published: October 2024

Abstract

Background.
Acute lung injury (ALI), ARDS and COVID-19 usually involve a “cytokine storm”. Insulin-like growth factor receptor 1 (IGF1R) maintains lung homeostasis and is implicated in these pulmonary inflammatory diseases. In mice, widespread IGF1R deficiency was reported to counteract respiratory inflammation and alveolar damage after bleomycin (BLM)-induced ALI.
Methods.
To explore the molecular mechanisms mediated by IGF1R signaling after BLM challenge, we performed RNA-sequencing in lungs of IGF1R-deficient mice after BLM or saline instillation, followed by differential expression and functional enrichment analyses. To further explore the findings, we performed protein immunodetection and DNA methylation measurements on lung sections and extracts, and analyses of primary MEFs lacking IGF1R.
Results.
Transcriptomic analysis identified differentially expressed genes between BLM-challenged and untreated control lungs, detecting biological processes and signaling pathways involved in ALI pathobiology. IGF1R depletion in BLM-challenged mice reversed large part of the transcriptional changes triggered by BLM, counteracting the transcriptomic profile of the inflammatory "cytokine storm". Data mining also identified changes in the expression of gene clusters with key roles in DNA damage, metabolic reprogramming, mitochondrial homeostasis, and epigenetics. These functional groups were deeply explored and further validated. IGF1R depleted MEFs exhibited decreased mitochondrial respiration and were protected against BLM-mediated morphological alterations, nuclear impairment and mitochondrial accumulation. Lung genomic DNA methylation levels in IGF1R-deficient BLM-challenged mice were found increased.
Conclusions.
These findings provide new insights into the molecular mechanisms underlying the attenuating effect of IGF1R deficiency on ALI, reinforce the important role of IGF1R in promoting ALI and postulate it as an epigenetic regulator in ARDS.
Effects of a Blended Face-to-Face and eHealth Lifestyle Intervention on Physical Activity, Diet, and Health Outcomes in Hong Kong Community-Dwelling Older Adults: A Randomized Controlled Trial
Authors: Duan Yanping , Yang Min , Wang Yanping , Cheng Shishi , Liang Wei , Lippke Sonia , Zhang Shiping , Chen Li 
Published: October 2024

Abstract

Background: Unhealthy lifestyle patterns, consisting of insufficient physical activity (PA) and unhealthy diets, are prevalent among older people, leading to detrimental health outcomes as the population ages. Traditional face-to-face was found to be resource-intensive and limited transfer. Exploring eHealth as a complementary approach to traditional interventions for older adults holds promise. However, whether a blended approach, integrating both face-to-face and eHealth interventions, could potentially enhance the effectiveness of interventions aimed at initiating and sustaining positive lifestyle behaviors was not tested in older individuals so far systematically. This study aims to examine the intervention effectiveness on health behaviors (i.e., PA and diet) and health outcomes among Hong Kong older adults.
Methods: A three-arm, single-blind, randomized controlled trial was conducted. 132 eligible older adults were recruited and randomly assigned into three groups. The blended intervention group received 10-week weekly two 60-minute face-to-face sessions with one for PA and one for diet, and two web-based sessions with one for PA and one for diet. The face-to-face intervention group received the same content and intensity like the blended group but as face-to-face sessions. The control group underwent biweekly telephone interviews. Data were collected at baseline, 10-week post-test, and 3-month follow-up assessment. All data were analyzed using IBM SPSS 29.0. A series of generalized linear mixed models were used to evaluate the effects of the intervention.
Results: The findings supported the effectiveness of blended interventions for PA and diet behaviors. Significant interaction effects were found on diet behaviors with large effect sizes. More participants in the blended group met the multiple health behavior guidelines than those in the face-to-face and control groups at T2 and T3. The results also revealed significant interaction effects on PA self-efficacy, PA planning, PA action control, fruit and vegetable intake self-efficacy, and meat, fish, egg, and alternatives intake action control. Significant intervention effects were found in agility and dynamic balance, and health-related quality of life.
Conclusions: This study provides empirical evidence for the effectiveness of blended interventions designed to promote PA, diet, and overall health outcomes among Hong Kong older adults.
Trial registration: ISRCTN, ISRCTN32329348. Registered 1 December 2023 - Retrospectively registered, https://doi.org/10.1186/ISRCTN32329348