bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole‐Genome Sequencing Analysis Using Next‐Generation Sequencing Data
Authors: Ho Chi Kent , Cui Xiaohui , Grubner Sharon , Larson Christopher A. , Wei Ying , Flook Paul K. 
Published: May 2016

AbstractNext‐generation sequencing (NGS) technologies have revolutionized the biosciences and become invaluable to the discovery of gene function and its involvement in disease conditions. The fast pace of innovation in NGS technologies has enabled the production of huge volumes of sequence data at progressively lower cost. However, the increasing throughput combined with the growing accessibility of these technologies poses significant challenges for downstream data analysis. Here, we provide an overview of NGS methods and key secondary analysis pipelines with a focus on technology provided by Illumina. As a case study, we highlight potential applications in cancer research. © 2016 by John Wiley & Sons, Inc.
Exploring Helpful Tensions between Divorce Mediators and Clients: A Relational Dialectical Analysis
Authors: Baitar Rachid , De Mol Jan , Rober Peter 
Published: May 2016

AbstractAddressing calls for more mediation process research, this qualitative study explored which contradictory tensions are in play in a helpful working relationship between mediators and their clients. Data were collected in semistructured interviews with divorce mediators (n = 12). Relational dialectical analysis detailed how divorce mediators varied practice styles along the central opposing pulls of client self‐determination and professional control, neutrality and engagement, and efficiency and exploration. The findings counterbalance traditional hallmarks of mediation and highlight a range of dynamic truths in which mediators operate. Methodological limitations and implications for research and practice are discussed.
A stationary wavelet transform approach to compressed spectrum sensing in cognitive radio
Authors: El‐Khamy Said E. , Abd‐el‐Malek Mina B. , Kamel Sara H. 
Published: May 2016

SummaryIn this paper, we introduce the implementation of the stationary wavelet transform in compressive sensing, particularly in spectrum sensing and edge detection in cognitive radio. We also review the different forms of the compressive sensing basis matrix, providing a comparative study of their performance, with emphasis on the matrices implementing the discrete wavelet transform, the stationary wavelet transform, and their multiscale counterparts. The results presented in this paper show that, using the stationary wavelet transform, we can reach the performance level and error rate obtained by the discrete wavelet transform using only half the samples required by the latter to attain the same performance. Copyright © 2016 John Wiley & Sons, Ltd.
Maternal separation followed by isolation‐housing differentially affects prepulse inhibition of the acoustic startle response in C57BL/6 mice
Authors: Bailoo Jeremy D. , Varholick Justin A. , Garza Xavier J. , Jordan Richard L. , Hintze Sara 
Published: May 2016

AbstractExposure to chronic stress is associated with an increased incidence of neuropsychiatric dysfunction. The current study evaluated two competing hypotheses, the cumulative stress and the match/mismatch hypothesis of neuropsychiatric dysfunction, using two paradigms relating to exposure to “stress”: pre‐weaning maternal separation and post‐weaning isolation‐housing. C57BL/6 offspring were reared under four conditions: typical animal facility rearing (AFR, control), early handling (EH, daily 15 min separation from dam), maternal separation (MS, daily 4 hr separation from dam), and maternal and peer separation (MPS, daily 4 hr separation from dam and from littermates). After weaning, mice were either housed socially (2–3/cage) or in isolation (1/cage) and then tested for prepulse inhibition in adulthood. Isolation‐housed MPS subjects displayed greater deficits in prepulse inhibition relative to socially‐housed MPS subjects while socially‐housed AFR subjects displayed greater deficits in prepulse inhibition relative to isolation‐housed AFR subjects. The results indicate that these treatment conditions represent a potentially valuable model for evaluating the match/mismatch hypothesis in regards to neuropsychiatric dysfunction.
The Arctic oscillation, climatic variability, and biotic factors influenced seedling dynamics in a Caribbean moist forest
Authors: McLaren Kurt , Monroe Setu , Wilson Byron 
Published: May 2016

AbstractWe assessed the influence of the Arctic oscillation (AO) on local climate (using data from 2004 to 2009), their influence and the effects of heterospecific density on seedling dynamics (from January 2006 to August 2009), using data from 120 25‐m2 subplots established in a moist tropical forest over limestone in Jamaica. The AO index (AOI) had a positive nonlinear relationship with mean monthly rainfall and the number of days with rain. Also, there was a significant increase in mean monthly atmospheric temperature in 2006, which coincided with a global temperature increase. Overall, at the community level, as temperature increased, mortality increased and then decreased. Also, mortality was significantly lower in plots with higher densities and those that experienced the positive phase of the AO. The effect of the AO on relative growth rate (RGR) of height (RGRh) varied as the AOI increased from negative to positive, while the number of days with rainfall had a positive effect on recruitment. However, these relationships differed during three six‐month and two 12‐month sample periods. There was a drought during the first period (dry season) during the negative phase of the AO; consequently, mortality was highest during this period. As the AOI increased (negative to positive), both mortality and RGRh declined while recruitment increased, culminating in a high‐recruitment event. In addition, as the number of days with rainfall increased, RGR of diameter (RGRd) values were more positive (indicating that moisture stress was alleviated). During the second period (wet season), mortality increased as seedling density increased (possibly due to increased competition). Additionally, elevated temperature had a significant negative effect on RGRh (again, possibly due to increased competition or due to elevated respiratory carbon loss at higher growth temperatures). After the first two censuses, temperature and the AO influenced dynamics marginally, and seedling heterospecific density became increasingly important (lower mortality at higher densities). At the population level, the number of days with rainfall was the most frequent predictor of dynamics followed by temperature, AO, density and rainfall, and they were largely beneficial.
The N‐Hydroxymethyl Group as a Traceless Activating Group for the CAL‐B‐Catalysed Ring Cleavage of β‐Lactams: A Type of Two‐Step Cascade Reaction
Authors: Forró Enikő , Galla Zsolt , Fülöp Ferenc 
Published: May 2016

AbstractAn efficient enzymatic two‐step cascade procedure has been devised for rapid access to diverse amino acids from N‐hydroxymethyl‐β‐lactams; representative amino acids include the antifungal agent cispentacin, intermediates for the taxol side‐chain, and assorted cathepsin inhibitors. When CAL‐B‐catalysed hydrolyses of racemic N‐hydroxymethyl‐β‐lactams were performed with H2O (0.5 equiv.) in iPr2O at 60 °C, relatively quick (vs. non‐activated counterparts) and enantioselective (E > 200) ring cleavage reactions took place. As the ring‐opened amino acids formed, the hydroxymethyl group, as a traceless activating group, underwent spontaneous in situ degradation. Consequently, the desired β‐amino acid and unreacted N‐hydroxymethyl‐β‐lactam enantiomers (ee > 95 %) were formed. The formation of polymers, induced by liberation of formaldehyde, was successfully restricted by the addition of benzylamine as a capture agent, to the enzymatic reactions.
Silver‐Catalyzed Denitrative Sulfonylation of β‐Nitrostyrenes: A Conve­nient Approach to (E)‐Vinyl Sulfones
Authors: Keshari Twinkle , Kapoorr Ritu , Yadav Lal Dhar S. 
Published: May 2016

AbstractThe first utilization of β‐nitrostyrenes (readily available by the Henry reaction) for a highly stereoselective, convenient, and catalytic synthesis of (E)‐vinyl sulfones at room temperature was investigated. The protocol involves efficient silver‐catalyzed denitrative radical cross‐coupling of β‐nitrostyrenes and sodium sulfinates by using potassium persulfate as an additive to complete the catalytic cycle.
Psychological correlates of acute postsurgical pain: A systematic review and meta‐analysis
Authors: Sobol‐Kwapinska M. , Bąbel P. , Plotek W. , Stelcer B. 
Published: May 2016

AbstractDue to the frequency of surgeries, acute postsurgical pain (APSP) is a common problem. However, the role of psychological factors in the experience of this kind of pain has not been well established. In this review, we focused on presurgical psychological factors associated with the experience of APSP. A systematic search of articles was performed using PsycARTICLES, PsycINFO, PubMed, MEDLINE, Scopus, Cochrane and DARE. For each study, we assessed the risk of bias, the level of evidence, the corresponding score points and the degree of association with APSP. Separate meta‐analyses were performed for the selected variables. Fifty‐three relevant publications were selected. Pain catastrophizing, optimism, expectation of pain, neuroticism, anxiety (state and trait), negative affect and depression were classified as likely associated with APSP. Only one of the analysed psychological variables – locus of control – was recognized as shown unlikely association with APSP. Results of meta‐analyses suggested that pain catastrophizing was most strongly linked with APSP. Results of the studies reviewed suggest that patients who do not exaggerate the negative aspects of the situation and who have positive expectation of the future before undergoing surgery report lower levels of APSP than patients who catastrophize pain and expect negative events in the future. An increasing interest in preoperative positive psychological variables has been observed over the last few years in studies of surgical patients.What does this review add?

Pain catastrophizing, optimism, expectation of pain, neuroticism, anxiety (state and trait), negative affect and depression were classified as likely associated with acute postsurgical pain, and locus of control was classified as unlikely associated with acute postsurgical pain.

Anxiety was the psychological variable most frequently measured before surgery.

Pain catastrophizing was most strongly linked with acute postsurgical pain.
Photoelectroanalytical Sensor Based on TiO2 Nanoparticles/Copper Tetrasulfonated Phthalocyanine for Detection of Dopamine Exploiting Light Emitting Diode Irradiation
Authors: Yotsumoto Neto Sakae , de Cássia Silva Luz Rita , Santos Damos Flávio 
Published: May 2016

AbstractIn this manuscript, a novel photoelectroanalytical sensor for detection of dopamine based on anatase TiO2 nanoparticles sensitized with copper tetrasulfonated phthalocyanine (CuTsPc/TiO2) was developed. The CuTsPc/TiO2 photoelectroanalytical sensor shows high anodic photocurrent under visible light emitting diode irradiation in comparison to pure anatase TiO2 nanoparticles. The CuTsPc/TiO2 composite shows a photocurrent about 2.5‐fold higher than those of pure TiO2 nanoparticles or CuTsPc modified electrode. The CuTsPc/TiO2 composite shows a low charge transfer resistance in comparison to its individual components as well as the impedance circle radius decreases significantly under UV light illumination. Under optimized conditions, the photoelectroanalytical sensor shows a linear response range from 4 up to 810 µmol L−1 with a sensitivity of 3.0 nA L µmol−1 and limit of detection of 0.5 µmol L−1 for the detection of dopamine. The photoelectrochemical sensor shows high photocurrent to dopamine in comparison to foreign species such as ascorbic acid, uric acid, glucose, K+, Na+, and Cl−.
Electrochemical Oxidation of the Antiretroviral Drug Nelfinavir on Modified Screen‐printed Electrodes
Authors: Asturias‐Arribas Laura , Delfino Mario Raúl , Alonso‐Lomillo M. Asunción , Domínguez‐Renedo Olga , Arcos‐Martínez M. Julia 
Published: May 2016

AbstractThis paper describes the voltammetric study of the electrochemical oxidation of the antiretroviral drug Nelfinavir using a screen‐printed sensor modified with carbon nanotubes. The performance of the sensor in the determination of the drug was characterized in terms of precision (RSD 5.05 %, n=5) and capability of detection (10.99±0.87 μM for α=β=0.05, n=5) under optimized voltammetric conditions of pH, accumulation time and potential, in the calibration range from 10 to 150 μM of NFV. In order to check the viability of the device, the determination of the analyte in pharmaceutical and biological samples was carried out and its performance was also validated comparing it with HPLC.
Large‐scale brain networks are distinctly affected in right and left mesial temporal lobe epilepsy
Authors: de Campos Brunno Machado , Coan Ana Carolina , Lin Yasuda Clarissa , Casseb Raphael Fernandes , Cendes Fernando 
Published: May 2016

AbstractMesial temporal lobe epilepsy (MTLE) with hippocampus sclerosis (HS) is associated with functional and structural alterations extending beyond the temporal regions and abnormal pattern of brain resting state networks (RSNs) connectivity. We hypothesized that the interaction of large‐scale RSNs is differently affected in patients with right‐ and left‐MTLE with HS compared to controls. We aimed to determine and characterize these alterations through the analysis of 12 RSNs, functionally parceled in 70 regions of interest (ROIs), from resting‐state functional‐MRIs of 99 subjects (52 controls, 26 right‐ and 21 left‐MTLE patients with HS). Image preprocessing and statistical analysis were performed using UF2C‐toolbox, which provided ROI‐wise results for intranetwork and internetwork connectivity. Intranetwork abnormalities were observed in the dorsal default mode network (DMN) in both groups of patients and in the posterior salience network in right‐MTLE. Both groups showed abnormal correlation between the dorsal‐DMN and the posterior salience, as well as between the dorsal‐DMN and the executive‐control network. Patients with left‐MTLE also showed reduced correlation between the dorsal‐DMN and visuospatial network and increased correlation between bilateral thalamus and the posterior salience network. The ipsilateral hippocampus stood out as a central area of abnormalities. Alterations on left‐MTLE expressed a low cluster coefficient, whereas the altered connections on right‐MTLE showed low cluster coefficient in the DMN but high in the posterior salience regions. Both right‐ and left‐MTLE patients with HS have widespread abnormal interactions of large‐scale brain networks; however, all parameters evaluated indicate that left‐MTLE has a more intricate bihemispheric dysfunction compared to right‐MTLE. Hum Brain Mapp 37:3137–3152, 2016. © 2016 The Authors Human Brain Mapping Published by Wiley Periodicals, Inc.
The ecohydrological imprint of deforestation in the semiarid Chaco: insights from the last forest remnants of a highly cultivated landscape
Authors: Giménez Raúl , Mercau Jorge , Nosetto Marcelo , Páez Ricardo , Jobbágy Esteban 
Published: May 2016

AbstractThe semiarid Chaco plains present one of the highest rates of forest clearing and agricultural expansion of the world. In other semiarid plains, such massive vegetation replacements initiated a groundwater recharge and salt mobilization process that, after decades, raised regional water tables and salts to the surface, degrading agricultural and natural ecosystems. Indirect evidence suggests that this process (known as dryland salinity) began in the Chaco plains. Multiple approaches (deep soil profiles, geoelectric surveys and monitoring of groundwater salinity, level and isotopic composition) were combined to assess the dryland salinity status in one of the oldest and most active agricultural hotspots of the region, where isolated forest remnants occupy an extremely flat cultivated matrix. Full vadose moisture and chloride profiles from paired agriculture‐forest stands (17 profiles, six sites) revealed the following: a generalized onset of deep drainage with cultivation (32 to >87 mm year−1), full leaching of native chloride pools (13.7 ± 2.5 kg m−2) down to the water table after >40 years following clearing and differential groundwater table rises (0.7 to 2 m shallower water tables under agriculture than under neighbouring forests). Continuous level monitoring showed abrupt water table rises under annual crops (up to 2.6 m in 15 days) not seen under forests or pastures. Varying deep drainage rates and groundwater isotopic composition under agricultural plots suggest that these pulses are strongly modulated by crop choices and sequences. In contrast to other dryland salinity‐affected areas of the world, forest remnants in the study area (10–20% of the area) are not only surviving the observed hydrological shifts but also sustaining active salty groundwater transpirative discharge, as evidenced by continuous water table records. The overall impact of these forest remnants on lowering neighbouring water tables would be limited by the low hydraulic conductivity of the sediments. As highly cultivated areas of the Chaco evolve to new hydrological conditions of shallower saline water tables, innovative crop rotations that minimize recharge, enhance transpirative discharge and tolerate salinity will be needed. Copyright © 2016 John Wiley & Sons, Ltd.
Highly sensitive SERS analysis of the cyclic Arg–Gly–Asp peptide ligands of cells using nanogap antennas
Authors: Portela Alejandro , Yano Taka‐aki , Santschi Christian , Martin Olivier J. F. , Tabata Hitoshi , Hara Masahiko 
Published: May 2016

The cyclic RGD (cRGD) peptide ligands of cells have become widely used for treating several cancers. We report a highly sensitive analysis of c(RGDfC) using surface enhanced Raman spectroscopy (SERS) using single dimer nanogap antennas in aqueous environment. Good agreement between characteristic peaks of the SERS and the Raman spectra of bulk c(RGDfC) with its peptide's constituents were observed. The exhibited blinking of the SERS spectra and synchronization of intensity fluctuations, suggest that the SERS spectra acquired from single dimer nanogap antennas was dominated by the spectrum of single to a few molecules. SERS spectra of c(RGDfC) could be used to detect at the nanoscale, the cells' transmembrane proteins binding to its ligand.
SERS of cyclic RGD on nanogap antenna.magnified imageSERS of cyclic RGD on nanogap antenna.
Multiparametric evaluation of hindlimb ischemia using time‐series indocyanine green fluorescence imaging
Authors: Guang Huizhi , Cai Chuangjian , Zuo Simin , Cai Wenjuan , Zhang Jiulou , Luo Jianwen 
Published: May 2016

Peripheral arterial disease (PAD) can further cause lower limb ischemia. Quantitative evaluation of the vascular perfusion in the ischemic limb contributes to diagnosis of PAD and preclinical development of new drug. In vivo time‐series indocyanine green (ICG) fluorescence imaging can noninvasively monitor blood flow and has a deep tissue penetration. The perfusion rate estimated from the time‐series ICG images is not enough for the evaluation of hindlimb ischemia. The information relevant to the vascular density is also important, because angiogenesis is an essential mechanism for post‐ischemic recovery. In this paper, a multiparametric evaluation method is proposed for simultaneous estimation of multiple vascular perfusion parameters, including not only the perfusion rate but also the vascular perfusion density and the time‐varying ICG concentration in veins. The target method is based on a mathematical model of ICG pharmacokinetics in the mouse hindlimb. The regression analysis performed on the time‐series ICG images obtained from a dynamic reflectance fluorescence imaging system. The results demonstrate that the estimated multiple parameters are effective to quantitatively evaluate the vascular perfusion and distinguish hypo‐perfused tissues from well‐perfused tissues in the mouse hindlimb. The proposed multiparametric evaluation method could be useful for PAD diagnosis.
The estimated perfusion rate and vascular perfusion density maps (left) and the time‐varying ICG concentration in veins of the ankle region (right) of the normal and ischemic hindlimbs.magnified imageThe estimated perfusion rate and vascular perfusion density maps (left) and the time‐varying ICG concentration in veins of the ankle region (right) of the normal and ischemic hindlimbs.
Photosensitized singlet oxygen generation and detection: Recent advances and future perspectives in cancer photodynamic therapy
Authors: Li Buhong , Lin Lisheng , Lin Huiyun , Wilson Brian C. 
Published: May 2016

Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to produce reactive oxygen species (ROS) that kill malignant cells by apoptosis and/or necrosis, shut down the tumor microvasculature and stimulate the host immune system. The excited singlet state of oxygen (1O2) is recognized to be the main cytotoxic ROS generated during PDT for the majority of photosensitizers used clinically and for many investigational new agents, so that maximizing its production within tumor cells and tissues can improve the therapeutic response, and several emerging and novel approaches for this are summarized. Quantitative techniques for1O2production measurement during photosensitization are also of immense importance of value for both preclinical research and future clinical practice. In this review, emerging strategies for enhanced photosensitized1O2generation are introduced, while recent advances in direct detection and imaging of1O2luminescence are summarized. In addition, the correlation between cumulative1O2luminescence and PDT efficiency will be highlighted. Meanwhile, the validation of1O2luminescence dosimetry for PDT application is also considered. This review concludes with a discussion on future demands of1O2luminescence detection for PDT dosimetry, with particular emphasis on clinical translation.Eye‐catching color image for graphical abstract.magnified imageEye‐catching color image for graphical abstract.
Micro‐break activities at work to recover from daily work demands
Authors: Kim Sooyeol , Park YoungAh , Niu Qikun 
Published: May 2016

SummaryRecovery literature has focused predominantly on recovery processes outside the workplace during nonwork times. Considering a lack of research on momentary recovery at work, we examined four categories of micro‐break activities—relaxation, nutrition‐intake, social, and cognitive activities—as possible recovery mechanisms in the workplace. Using effort recovery and conservation of resources theories, we hypothesized that micro‐break activities attenuate the common stressor–strain relationship between work demands and negative affect. For 10 consecutive workdays, 86 South Korean office workers (842 data points) reported their specific daily work demands right after their lunch hour (Time 1) and then reported their engagement in micro‐break activities during the afternoon and negative affective state at the end of the workday (Time 2). As expected, relaxation and social activities reduced the effects of work demands on end‐of‐workday negative affect. Nutrition intake of beverages and snacks did not have a significant moderating effect. Post hoc analyses, however, revealed that only caffeinated beverages reduced work demands effects on negative affect. Unexpectedly, cognitive activities aggravated the effects of work demands on negative affect. The findings indicate not only the importance of taking micro‐breaks but also which types of break activities are beneficial for recovery. Implications, limitations, and future research directions are discussed. Copyright © 2016 John Wiley & Sons, Ltd.