bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anoxia and glucose supplementation preserve neutrophil viability and function
Authors: Monceaux Valérie , Chiche-Lapierre Clarisse , Chaput Catherine , Witko-Sarsat Véronique , Prevost Marie-Christine , Taylor Cormac T. , Ungeheuer Marie-Noelle , Sansonetti Philippe J. , Marteyn Benoit S. 
Published: July 2016

Key PointsAnoxia combined with glucose supplementation maintains viability of neutrophils for 20 hours without affecting their functions. Such conditioned neutrophils are suitable for efficient DNA transfection and transfusion.
Corticosteroids compared with intravenous immunoglobulin for the treatment of immune thrombocytopenia in pregnancy
Authors: Sun Dongmei , Shehata Nadine , Ye Xiang Y. , Gregorovich Sandra , De France Bryon , Arnold Donald M. , Shah Prakesh S. , Malinowski Ann Kinga 
Published: July 2016

Key Points
Maternal platelet count response was not different for IVIg and corticosteroids in this retrospective study of pregnant women with ITP. Neonatal outcomes were overall favorable and similar after treatment of maternal ITP with IVIg or corticosteroids.
Objectively measured physical activity during pregnancy: a study in obese and overweight women
Authors: McParlin Catherine , Robson Stephen C , Tennant Peter WG , Besson Hervé , Rankin Judith , Adamson Ashley J , Pearce Mark S , Bell Ruth 
Published: July 2016

Abstract

Background
Obese and overweight women may benefit from increased physical activity (PA) during pregnancy. There is limited published data describing objectively measured PA in such women.

Methods
A longitudinal observational study of PA intensity, type and duration using objective and subjective measurement methods. Fifty five pregnant women with booking body mass index (BMI) ≥ 25 kg/m2 were recruited from a hospital ultrasound clinic in North East England. 26 (47%) were nulliparous and 22 (40%) were obese (BMI ≥ 30 kg/m2). PA was measured by accelerometry and self report questionnaire at 13 weeks, 26 weeks and/or 36 weeks gestation. Outcome measures were daily duration of light, moderate or vigorous activity assessed by accelerometry; calculated overall PA energy expenditure, (PAEE), and PAEE within four domains of activity based on self report.

Results
At median 13 weeks gestation, women recorded a median 125 mins/day light activity and 35 mins/day moderate or vigorous activity (MVPA). 65% achieved the minimum recommended 30 mins/day MVPA. This proportion was maintained at 26 weeks (62%) and 36 weeks (71%). Women achieving more than 30 mins/day MVPA in the first trimester showed a significant reduction in duration of MVPA by the third trimester (11 mins/day, p = 0.003). Walking, swimming and floor exercises were the most commonly reported recreational activities but their contribution to estimated energy expenditure was small.

Conclusion
Overweight and obese pregnant women can achieve and maintain recommended levels of PA throughout pregnancy. Interventions to promote PA should target changes in habitual activities at work and at home, and in particular walking.
Implementation of blinded outcome assessment in the Effective Verruca Treatments trial (EverT) – lessons learned
Authors: Cockayne Sarah , Hewitt Catherine , Hashmi Farina , Hicks Kate , Concannon Michael , McIntosh Caroline , Thomas Kim , Hall Jill , Watson Judith , Torgerson David , Watt Ian 
Published: July 2016

AbstractBackgroundTrials using inadequate levels of blinding may report larger effect sizes than blinded studies. It has been suggested that blinded outcome assessment in open trials may in some cases be undertaken by assessments of photographs. The aim of this paper is to explore the effect of using different methods to assess the primary outcome in the EVerT (Effective Verruca Treatments) trial. It also aims to give an overview of the experiences of using digital photographs within the trial.MethodsWe undertook a secondary analysis to explore the effect of using three different methods to assess the primary outcome in the EVerT trial: assessment of digital photographs by blinded healthcare professionals; blinded healthcare professional assessment at the recruiting site and patient self‐report. The verruca clearance rates were calculated using the three different methods of assessment. A Cohen's kappa measure of inter‐rater agreement was used to assess the agreement between the methods. We also investigated the experiences of healthcare professionals using digital photographs within the trial.ResultsDigital photographs for 189 out of 240 (79 %) patients in the trial were received for outcome assessment. Of the 189 photographs, 30 (16 %) were uninterpretable. The overall verruca clearance rates were 21 % (43/202,) using the unblinded patient self‐reported outcome, 6 % (9/159,) using blinded assessment of digital photographs and 14 % (30/210,) using blinded outcome assessment at the site.ConclusionsDespite differences in the clearance rates found using different methods of outcome assessment, this did not change the original conclusion of the trial, that there is no evidence of a difference in effectiveness between cryotherapy and salicylic acid. Future trials using digital photographs should consider individual training needs at sites and have a backup method of assessment agreed a priori.Trial registrationISRCTN Registry ISRCTN18994246
Europe and North East Asia, a complementary couple?
Authors: Széll György 
Published: July 2016

AbstractsEuropean and North East Asian histories are intermingled since more than 200 years - full of conflicts, but also of cooperation. An issue over the past couple of decades is about European, Asian or universal values. The article confronts these different approaches and comes to the conclusion that diversity and complexity ask for a multidisciplinary approach.Die europäische und nordostasiatische Geschichte vermengen sich seit mehr als zweitausend Jahren. - voller Konflikte, aber auch Zusammenarbeit. Ein besonderes Problem ist die Frage nach europäischen, asiatischen oder universellen Werten. Der Beitrag stellt diese verschiedenen Ansätze gegenüber und kommt zu dem Ergebnis, dass Vielfalt und Komplexität einen multi-disiziplinären Ansatz erfordern.
Phase II Multicenter, Randomized, Double-Blind Controlled Study of Efficacy and Safety of Umbilical Cord–Derived Mesenchymal Stromal Cells in the Prophylaxis of Chronic Graft-Versus-Host Disease After HLA-Haploidentical Stem-Cell Transplantation
Authors: Gao Lei , Zhang Yanqi , Hu Baoyang , Liu Jia , Kong Peiyan , Lou Shifeng , Su Yi , Yang Tonghua , Li Huimin , Liu Yao , Zhang Cheng , Gao Li , Zhu Lidan , Wen Qin , Wang Ping , Chen Xinghua , Zhong Jiangfan , Zhang Xi 
Published: July 2016

Purpose Although mesenchymal stromal cells (MSCs) possess immunomodulatory properties and exhibit promising efficacy against chronic graft-versus-host disease (cGVHD), little is known about the efficacy of MSCs in the prophylaxis of cGVHD after HLA-haploidentical hematopoietic stem-cell transplantation (HLA-haplo HSCT). Patients and Methods In this multicenter, double-blind, randomized controlled trial, we investigated the incidence and severity of cGVHD among patients, and the changes in T, B, and natural killer (NK) cells after the repeated infusion of MSCs. Results The 2-year cumulative incidence of cGVHD in the MSCs group was 27.4% (95% CI, 16.2% to 38.6%), compared with 49.0% (95% CI, 36.5% to 61.5%) in the non-MSCs control group (P = .021). Seven patients in the non-MSCs control group had severe lung cGVHD, but no patients in the MSCs group developed typical lung cGVHD (P = .047). After the MSC infusions, increasing memory B lymphocytes and regulatory T cells, as well as the ratio of type 1 T helper to type 2 T helper cells, were observed, whereas the number of NK cells decreased. Conclusion Our findings suggest that the repeated infusion of MSCs might inhibit cGVHD symptoms in patients after HLA-haplo HSCT, accompanied by changes in the numbers and subtypes of T, B, and NK cells, leading to the acquisition of immune tolerance.
Improved Outcomes With Retinoic Acid and Arsenic Trioxide Compared With Retinoic Acid and Chemotherapy in Non–High-Risk Acute Promyelocytic Leukemia: Final Results of the Randomized Italian-German APL0406 Trial
Authors: Platzbecker Uwe , Avvisati Giuseppe , Cicconi Laura , Thiede Christian , Paoloni Francesca , Vignetti Marco , Ferrara Felicetto , Divona Mariadomenica , Albano Francesco , Efficace Fabio , Fazi Paola , Sborgia Marco , Di Bona Eros , Breccia Massimo , Borlenghi Erika , Cairoli Roberto , Rambaldi Alessandro , Melillo Lorella , La Nasa Giorgio , Fiedler Walter , Brossart Peter , Hertenstein Bernd , Salih Helmut R. , Wattad Mohammed , Lübbert Michael , Brandts Christian H. , Hänel Mathias , Röllig Christoph , Schmitz Norbert , Link Hartmut , Frairia Chiara , Pogliani Enrico Maria , Fozza Claudio , D’Arco Alfonso Maria , Di Renzo Nicola , Cortelezzi Agostino , Fabbiano Francesco , Döhner Konstanze , Ganser Arnold , Döhner Hartmut , Amadori Sergio , Mandelli Franco , Ehninger Gerhard , Schlenk Richard F. , Lo-Coco Francesco 
Published: July 2016

Purpose The initial results of the APL0406 trial showed that the combination of all- trans-retinoic acid (ATRA) and arsenic trioxide (ATO) is at least not inferior to standard ATRA and chemotherapy (CHT) in first-line therapy of low- or intermediate-risk acute promyelocytic leukemia (APL). We herein report the final analysis on the complete series of patients enrolled onto this trial. Patients and Methods The APL0406 study was a prospective, randomized, multicenter, open-label, phase III noninferiority trial. Eligible patients were adults between 18 and 71 years of age with newly diagnosed, low- or intermediate-risk APL (WBC at diagnosis ≤ 10 × 109/L). Overall, 276 patients were randomly assigned to receive ATRA-ATO or ATRA-CHT between October 2007 and January 2013. Results Of 263 patients evaluable for response to induction, 127 (100%) of 127 patients and 132 (97%) of 136 patients achieved complete remission (CR) in the ATRA-ATO and ATRA-CHT arms, respectively ( P = .12). After a median follow-up of 40.6 months, the event-free survival, cumulative incidence of relapse, and overall survival at 50 months for patients in the ATRA-ATO versus ATRA-CHT arms were 97.3% v 80%, 1.9% v 13.9%, and 99.2% v 92.6%, respectively ( P < .001, P = .0013, and P = .0073, respectively). Postinduction events included two relapses and one death in CR in the ATRA-ATO arm and two instances of molecular resistance after third consolidation, 15 relapses, and five deaths in CR in the ATRA-CHT arm. Two patients in the ATRA-CHT arm developed a therapy-related myeloid neoplasm. Conclusion These results show that the advantages of ATRA-ATO over ATRA-CHT increase over time and that there is significantly greater and more sustained antileukemic efficacy of ATO-ATRA compared with ATRA-CHT in low- and intermediate-risk APL.
Barriers to Quality End-of-Life Care for Patients With Blood Cancers
Authors: Odejide Oreofe O. , Cronin Angel M. , Condron Nolan B. , Fletcher Sean A. , Earle Craig C. , Tulsky James A. , Abel Gregory A. 
Published: July 2016

Purpose Patients with blood cancers have been shown to receive suboptimal care at the end of life (EOL) when assessed with standard oncology quality measures (eg, no chemotherapy ≤ 14 days before death). As they were developed primarily for solid tumors, it is unclear if these measures are appropriate for patients with hematologic malignancies. Moreover, barriers to high-quality EOL care for this specific patient population are largely unknown. Methods In 2015, we asked a national cohort of hematologic oncologists about the acceptability of eight standard EOL quality measures. Building on prior qualitative work, we prespecified that measures achieving agreement among at least 55% of respondents would be considered acceptable. We also explored perspectives regarding barriers to quality EOL care. Results We received 349 surveys (response rate = 57.3%). Six of the standard measures met the threshold of acceptability, and four were acceptable to > 75% of respondents: hospice admission > 7 days before death, no chemotherapy ≤ 14 days before death, no intubation in the last 30 days of life, and no cardiopulmonary resuscitation in the last 30 days of life. The highest-ranked barriers to quality EOL care reported were “unrealistic patient expectations” (97.3%), “clinician concern about taking away hope” (71.3%), and “unrealistic clinician expectations” (59.0%). Conclusion In this large national cohort of hematologic oncologists, standard EOL quality measures were highly acceptable. The top barrier to quality EOL care reported was unrealistic patient expectations, which may be best addressed with more timely and effective advance care discussions.
Is FDA-Approved Bevacizumab Cost-Effective When Included in the Treatment of Platinum-Resistant Recurrent Ovarian Cancer?
Authors: Chappell Nicole P. , Miller Caela R. , Fielden Aaron D. , Barnett Jason C. 
Published: July 2016

Purpose: Although the Food and Drug Administration has approved incorporation of bevacizumab (BEV) into the treatment of platinum-resistant ovarian cancer (PROC), cost-value measures are an essential consideration, as evidenced by the recent ASCO Value Framework initiative. We assessed the cost-effectiveness and reviewed the net health benefit (NHB) of this expensive treatment. Methods: A cost-effectiveness decision model was constructed using results from a phase III trial comparing BEV plus cytotoxic chemotherapy with chemotherapy alone in patients with PROC. The Avastin Use in Platinum-Resistant Epithelial Ovarian Cancer (AURELIA) trial demonstrated improvement in progression-free survival and quality of life in patients receiving BEV. Costs, paracentesis rates, and adverse events were incorporated, including subgroup analysis of different partner chemotherapy agents. Results: Inclusion of BEV in the treatment of platinum-resistant recurrent ovarian cancer meets the common willingness-to-pay incremental cost-effectiveness ratio (ICER) threshold of $100,000 per progression-free life-year saved (LYS) for 15-mg/kg dosing and approaches this threshold for 10-mg/kg dosing, with an ICER of $160,000. In sensitivity analysis, reducing the cost of BEV by 13% (from $9,338 to $8,100 per cycle) allows 10-mg/kg dosing to reach a $100,000 ICER. Exploratory analysis of different BEV chemotherapy partners showed an ICER of $76,000 per progression-free LYS (6.5-month progression-free survival improvement) and $54,000 per LYS (9.1-month overall survival improvement) for the addition of BEV to paclitaxel once per week. Using the ASCO framework for value assessment, the NHB score for BEV plus paclitaxel once per week is 48. Conclusion: Using a willingness-to-pay threshold of $100,000 ICER, the addition of BEV to chemotherapy either demonstrates or approaches cost-effectiveness and NHB when added to the treatment of patients with PROC.
A Novel Phenotype of Familial Hyperaldosteronism Type III: Concurrence of Aldosteronism and Cushing’s Syndrome
Authors: Tong Anli , Liu Guanghua , Wang Fen , Jiang Jun , Yan Zhaoli , Zhang Dianxi , Zhang Yinsheng , Cai Jun 
Published: July 2016

Context:
To date, all the familial hyperaldosteronism type III (FH-III) patients reported presenting with typical primary aldosteronism (PA), without showing other adrenal hormone abnormalities.

Objective:
This study characterized a novel phenotype of FH-III and explored the possible pathogenesis.

Patients and Methods:
A male patient presented with severe hypertension and hypokalemia at the age of 2 years and developed Cushing’s syndrome at 20 years. He was diagnosed with PA and Cushing’s syndrome on the basis of typical biochemical findings. He had massive bilateral adrenal hyperplasia and underwent left adrenalectomy. KCNJ5 was sequenced, and secretion of aldosterone and cortisol were observed both in vivo and in vitro.

Results:
A heterozygous germline p.Glu145Gln mutation of KCNJ5 was identified. ARMC5, PRKAR1A, PDE8B, PDE11A, and PRKACA genes and β-catenin, P53 immunoactivity were normal in the adrenal. CYP11B2 was highly expressed, whereas mRNA expression of CYP11B1, CYP17A1, and STAR was relatively low in the hyperplastic adrenal, compared with normal adrenal cortex and other adrenal diseases. In the primary cell culture of the resected hyperplastic adrenal, verapamil and nifedipine, two calcium channel blockers, markedly inhibited the secretion of both aldosterone and cortisol and the mRNA expression of CYP11B1, CYP11B2, CYP17A1, and STAR.

Conclusions:
We presented the first FH-III patient who had both severe PA and Cushing’s syndrome. Hypersecretion of cortisol might be ascribed to overly large size of the hyperplastic adrenal because CYP11B1 expression was relatively low in his adrenal. Like aldosterone, synthesis and secretion of cortisol in the mutant adrenal may be mediated by voltage-gated Ca2+ channels.
Patriarchy from Margin to Center: Discipline, Territoriality, and Cruelty in the Apocalyptic Phase of Capital
Authors: Segato Rita Laura 
Published: July 2016

Colonial intervention in what I have called the village world (Segato 2015) has minoritized everything regarding women. The term minoritization refers to women's representation and collective position in social thought: minoritizing women means relegating all issues related to them to the realm of the intimate, the private, the particular, that is, treating theirs as minority (and consequently minor) issues. Such minoritization of women is the result of a historical transition to colonial modernity.
This transition was driven at first by the process of conquest and colonization led by the overseas metropolis and then later by the administration of the Criollo-elite constructed state. Therefore, this process can also be described as criollization. The transition to colonial-modernity—the current rapid expansion of the state-business-media-Christianity front—intrudes into the mode of existence of the village world and has a massive impact on interpersonal relationships and the organization of gender relations in societies that are still governed by communitarian or collectivist models of coexistence, or where a communitarian fabric can still be found that, if not unscathed, is at least recognizable and vital. In its wake, that transition integrates different peoples in the mass of the national “citizenry.” Considering transformations in the gender system, the history of the patriarchal structure is indispensable for understanding the social shift introduced by modernity. Correctly reading what this transition has meant and how intervention has readjusted and eroded preexisting hierarchies allows us to understand a number of current phenomena that affect society as a whole, phenomena that cannot be expressed or contained by the term the woman problem.
Development of a diet-induced murine model of diabetes featuring cardinal metabolic and pathophysiological abnormalities of type 2 diabetes
Authors: Morris Jodie L. , Bridson Tahnee L. , Alim Md Abdul , Rush Catherine M. , Rudd Donna M. , Govan Brenda L. , Ketheesan Natkunam 
Published: July 2016

ABSTRACT
The persistent rise in global incidence of type 2 diabetes (T2D) continues to have significant public health and economic implications. The availability of relevant animal models of T2D is critical to elucidating the complexity of the pathogenic mechanisms underlying this disease and the implications this has on susceptibility to T2D complications. Whilst many high-fat diet-induced rodent models of obesity and diabetes exist, growing appreciation of the contribution of high glycaemic index diets on the development of hyperglycaemia and insulin resistance highlight the requirement for animal models that more closely represent global dietary patterns reflective of modern society. To that end, we sought to develop and validate a murine model of T2D based on consumption of an energy-dense diet containing moderate levels of fat and a high glycaemic index to better reflect the aetiopathogenesis of T2D. Male C57BL/6 mice were fed an energy-dense (ED) diet and the development of pathological features used in the clinical diagnosis of T2D was assessed over a 30-week period. Compared with control mice, 87% of mice fed an ED diet developed pathognomonic signs of T2D including glucose intolerance, hyperglycaemia, glycosylated haemoglobin (HbA1c) and glycosuria within 30 weeks. Furthermore, dyslipidaemia, chronic inflammation, alterations in circulating leucocytes and renal impairment were also evident in ED diet-fed mice compared with mice receiving standard rodent chow. Longitudinal profiling of metabolic and biochemical parameters provide support of an aetiologically and clinically relevant model of T2D that will serve as a valuable tool for mechanistic and therapeutic studies investigating the pathogenic complications of T2D.
B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis inDrosophilamale meiosis
Authors: Hayashi Daisuke , Tanabe Karin , Katsube Hiroka , Inoue Yoshihiro H. 
Published: July 2016

ABSTRACTIn higher eukaryotes, nuclear envelope (NE) disassembly allows chromatin to condense and spindle microtubules to access kinetochores. The nuclear lamina, which strengthens the NE, is composed of a polymer meshwork made of A- and B-type lamins. We found that the B-type lamin (Lam) is not fully disassembled and continues to localize along the spindle envelope structure during Drosophila male meiosis I, while the A-type lamin (LamC) is completely dispersed throughout the cytoplasm. Among the nuclear pore complex proteins, Nup107 co-localized with Lam during this meiotic division. Surprisingly, Lam depletion resulted in a higher frequency of cytokinesis failure in male meiosis. We also observed the similar meiotic phenotype in Nup107-depleted cells. Abnormal localization of Lam was found in the Nup-depleted cells at premeiotic and meiotic stages. The central spindle microtubules became abnormal and recruitment of a contractile ring component to the cleavage sites was disrupted in Lam-depleted cells and Nup107-depleted cells. Therefore, we speculate that both proteins are required for a reinforcement of the spindle envelope, which supports the formation of central spindle microtubules essential for cytokinesis in Drosophila male meiosis.
Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination
Authors: Shamir Eliah R. , Coutinho Kester , Georgess Dan , Auer Manfred , Ewald Andrew J. 
Published: July 2016

ABSTRACT
Dissemination is the process by which cells detach and migrate away from a multicellular tissue. The epithelial-to-mesenchymal transition (EMT) conceptualizes dissemination in a stepwise fashion, with downregulation of E-cadherin leading to loss of intercellular junctions, induction of motility, and then escape from the epithelium. This gain of migratory activity is proposed to be mutually exclusive with proliferation. We previously developed a dissemination assay based on inducible expression of the transcription factor Twist1 and here utilize it to characterize the timing and dynamics of intercellular adhesion, proliferation and migration during dissemination. Surprisingly, Twist1+ epithelium displayed extensive intercellular junctions, and Twist1– luminal epithelial cells could still adhere to disseminating Twist1+ cells. Although proteolysis and proliferation were both observed throughout dissemination, neither was absolutely required. Finally, Twist1+ cells exhibited a hybrid migration mode; their morphology and nuclear deformation were characteristic of amoeboid cells, whereas their dynamic protrusive activity, pericellular proteolysis and migration speeds were more typical of mesenchymal cells. Our data reveal that epithelial cells can disseminate while retaining competence to adhere and proliferate.