bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: Dual roles of miR-374a by modulated c-Jun respectively targets CCND1-inducing PI3K/AKT signal and PTEN-suppressing Wnt/β-catenin signaling in non-small-cell lung cancer
Authors: Zhao Mengyang , Xu Ping , Liu Zhen , Zhen Yan , Chen Yiyu , Liu Yiyi , Fu Qiaofen , Deng Xiaojie , Liang Zixi , Li Yonghao , Lin Xian , Fang Weiyi 
Published: January 2018

AbstractMiR-374a appears to play a complex role in non-small-cell lung cancer (NSCLC). Here, we demonstrate a dual role for miR-374a in NSCLC pathogenesis. The effects and modulatory mechanisms of miR-374a on cell growth, migration, invasion, and in vivo tumorigenesis and metastasis in nude mice were also analyzed. The expression of miR-374a was examined in NSCLC and non-cancerous lung tissues by quantitative real-time reverse transcription-PCR (qRT-PCR), and in situ hybridization, respectively. miR-374a directly targets CCND1 and inactivates PI3K/AKT and Ras-mediated cell cycle signalings, as well as epithelial–mesenchymal transition (EMT). This not only dramatically suppressed cell growth, migration, invasion,and metastasis, but also elevated A549 and pc-9 NSCLC cell sensitivity to cisplatin (DDP) while increasing survival time of tumor-bearing mice. Interestingly, miR-374a serves an inverse function in SPCA-1 and H1975 NSCLC cells by directly targeting PTEN to activate Wnt/β-catenin and Ras signalings and its downstream cascade signals. Surprisingly, transcription factor c-Jun bound to the promoter region of human miR-374a and suppressed miR-374a in A549 and pc-9 cells while inducing it in SPCA-1 and H1975 cells. Increased levels of miR-374a appeared to serve a protective role by targeting CCND1 in early-stage NSCLC (Stages I and II). Inversely, increased miR-374a was an unfavorable factor when targeting PTEN in more advanced staged NSCLC patients. Our studies are the first to demonstrate that miR-374a plays divergent roles in NSCLC pathogenesis at different stages of the disease and implicate the potential application of miR-374a targeting for cancer therapy.
A novel ruthenium complex with xanthoxylin induces S-phase arrest and causes ERK1/2-mediated apoptosis in HepG2 cells through a p53-independent pathway
Authors: de Carvalho Nanashara C. , Neves Sara P. , Dias Rosane B. , Valverde Ludmila de F. , Sales Caroline B. S. , Rocha Clarissa A. G. , Soares Milena B. P. , dos Santos Edjane R. , Oliveira Regina M. M. , Carlos Rose M. , Nogueira Paulo C. L. , Bezerra Daniel P. 
Published: January 2018

AbstractRuthenium-based compounds have gained great interest due to their potent cytotoxicity in cancer cells; however, much of their potential applications remain unexplored. In this paper, we report the synthesis of a novel ruthenium complex with xanthoxylin (RCX) and the investigation of its cellular and molecular action in human hepatocellular carcinoma HepG2 cells. We found that RCX exhibited a potent cytotoxic effect in a panel of cancer cell lines in monolayer cultures and in a 3D model of multicellular cancer spheroids formed from HepG2 cells. This compound is detected at a high concentration in the cell nuclei, induces DNA intercalation and inhibits DNA synthesis, arresting the cell cycle in the S-phase, which is followed by the activation of the caspase-mediated apoptosis pathway in HepG2 cells. Gene expression analysis revealed changes in the expression of genes related to cell cycle control, apoptosis and the MAPK pathway. In addition, RCX induced the phosphorylation of ERK1/2, and pretreatment with U-0126, an MEK inhibitor known to inhibit the activation of ERK1/2, prevented RCX-induced apoptosis. In contrast, pretreatment with a p53 inhibitor (cyclic pifithrin-α) did not prevent RCX-induced apoptosis, indicating the activation of a p53-independent apoptosis pathway. RCX also presented a potent in vivo antitumor effect in C.B-17 SCID mice engrafted with HepG2 cells. Altogether, these results indicate that RCX is a novel anticancer drug candidate.
Fused electron deficient semiconducting polymers for air stable electron transport
Authors: Onwubiko Ada , Yue Wan , Jellett Cameron , Xiao Mingfei , Chen Hung-Yang , Ravva Mahesh Kumar , Hanifi David A. , Knall Astrid-Caroline , Purushothaman Balaji , Nikolka Mark , Flores Jean-Charles , Salleo Alberto , Bredas Jean-Luc , Sirringhaus Henning , Hayoz Pascal , McCulloch Iain 
Published: January 2018

AbstractConventional semiconducting polymer synthesis typically involves transition metal-mediated coupling reactions that link aromatic units with single bonds along the backbone. Rotation around these bonds contributes to conformational and energetic disorder and therefore potentially limits charge delocalisation, whereas the use of transition metals presents difficulties for sustainability and application in biological environments. Here we show that a simple aldol condensation reaction can prepare polymers where double bonds lock-in a rigid backbone conformation, thus eliminating free rotation along the conjugated backbone. This polymerisation route requires neither organometallic monomers nor transition metal catalysts and offers a reliable design strategy to facilitate delocalisation of frontier molecular orbitals, elimination of energetic disorder arising from rotational torsion and allowing closer interchain electronic coupling. These characteristics are desirable for high charge carrier mobilities. Our polymers with a high electron affinity display long wavelength NIR absorption with air stable electron transport in solution processed organic thin film transistors.
Dual-band Circular Polarizer Based on Simultaneous Anisotropy and Chirality in Planar Metamaterial
Authors: Zhao Yizhe , Qing Anyong , Meng Yang , Song Zelin , Lin Chuan 
Published: January 2018

AbstractMetamaterial of dual-square array is proposed to design a dual-band circular polarizer. The novel design of asymmetric unit cell and layout of duplicate arrays significantly enhances the coupling between electric and magnetic fields. Simulation and measurement results show that the polarizer presents wide angle circular dichroism and circular birefringence. Moreover, the polarization conversion of the proposed metamaterial changes with frequency, incident angle, and polarization of incident waves. The fundamental mechanism behind is concluded to be the angle-dependent chirality and dispersion of our novel design.
Structure and hydrogen bonding at the limits of liquid water stability
Authors: Cipcigan Flaviu , Sokhan Vlad , Martyna Glenn , Crain Jason 
Published: January 2018

AbstractLiquid water exhibits unconventional behaviour across its wide range of stability – from its unusually high liquid-vapour critical point down to its melting point and below where it reaches a density maximum and exhibits negative thermal expansion allowing ice to float. Understanding the molecular underpinnings of these anomalies presents a challenge motivating the study of water for well over a century. Here we examine the molecular structure of liquid water across its range of stability, from mild supercooling to the negative pressure and high temperature regimes. We use a recently-developed, electronically-responsive model of water, constructed from gas-phase molecular properties and incorporating many-body, long-range interactions to all orders; as a result the model has been shown to have high transferability from ice to the supercritical regime. We report a link between the anomalous thermal expansion of water and the behaviour of its second coordination shell and an anomaly in hydrogen bonding, which persists throughout liquid water’s range of stability – from the high temperature limit of liquid water to its supercooled regime.
Adhesive Leaf Created by a Corona Discharge
Authors: Lee Wonseok , Son Jongsang , Kim Seonghyun , Yang Dongmin , Choi Seungyeop , Watanabe Rodrigo Akira , Hwang Kyo Seon , Lee Sang Woo , Lee Gyudo , Yoon Dae Sung 
Published: January 2018

AbstractHere, we report a new concept of both the adhesive manner and material, named “adhesive leaf (AL),” based on the leaf of the plant Heteropanax fragrans. The treatment of the corona discharge on the leaf surface can cause the nano-/microdestruction of the leaf epidermis, resulting in an outward release of sap. The glucose-containing sap provided the AL with a unique ability to stick to various substrates such as steel, polypropylene, and glass. Moreover, we reveal that the AL adhesion strength depends on the AL size, as well as the corona-discharge intensity. Conventional adhesives, such as glue and bond, lose their adhesive property and leave dirty residues upon the removal of the attached material. Unlike the conventional methods, the AL is advantageous as it can be repeatedly attached and detached thoroughly until the sap liquid is exhausted; its adhesive ability is maintained for at least three weeks at room temperature. Our findings shed light on a new concept of a biodegradable adhesive material that is created by a simple surface treatment.
Downregulation of glutamic acid decarboxylase in Drosophila TDP-43-null brains provokes paralysis by affecting the organization of the neuromuscular synapses
Authors: Romano Giulia , Holodkov Nikola , Klima Raffaella , Grilli Federica , Guarnaccia Corrado , Nizzardo Monica , Rizzo Federica , Garcia Rodolfo , Feiguin Fabian 
Published: January 2018

AbstractAmyotrophic lateral sclerosis is a progressive neurodegenerative disease that affects the motor system, comprised of motoneurons and associated glia. Accordingly, neuronal or glial defects in TDP-43 function provoke paralysis due to the degeneration of the neuromuscular synapses in Drosophila. To identify the responsible molecules and mechanisms, we performed a genome wide proteomic analysis to determine differences in protein expression between wild-type and TDP-43-minus fly heads. The data established that mutant insects presented reduced levels of the enzyme glutamic acid decarboxylase (Gad1) and increased concentrations of extracellular glutamate. Genetic rescue of Gad1 activity in neurons or glia was sufficient to recuperate flies locomotion, synaptic organization and glutamate levels. Analogous recovery was obtained by treating TDP-43-null flies with glutamate receptor antagonists demonstrating that Gad1 promotes synapses formation and prevents excitotoxicity. Similar suppression of TDP-43 provoked the downregulation of GAD67, the Gad1 homolog protein in human neuroblastoma cell lines and analogous modifications were observed in iPSC-derived motoneurons from patients carrying mutations in TDP-43, uncovering conserved pathological mechanisms behind the disease.
Foxj1 expressing ependymal cells do not contribute new cells to sites of injury or stroke in the mouse forebrain
Authors: Muthusamy Nagendran , Brumm Andrew , Zhang Xuying , Carmichael S. Thomas , Ghashghaei H. Troy 
Published: January 2018

AbstractThe stem cell source of neural and glial progenitors in the periventricular regions of the adult forebrain has remained uncertain and controversial. Using a cell specific genetic approach we rule out Foxj1+ ependymal cells as stem cells participating in neurogenesis and gliogenesis in response to acute injury or stroke in the mouse forebrain. Non stem- and progenitor-like responses of Foxj1+ ependymal cells to injury and stroke remain to be defined and investigated.
Distinctive expression of T cell guiding molecules in human autoimmune lymph node stromal cells upon TLR3 triggering
Authors: Hähnlein Janine S. , Ramwadhdoebe Tamara H. , Semmelink Johanna F. , Choi Ivy Y. , Berger Ferco H. , Maas Mario , Gerlag Danielle M. , Tak Paul P. , Geijtenbeek Teunis B. H. , van Baarsen Lisa G. M. 
Published: January 2018

AbstractInfections are implicated in autoimmunity. Autoantibodies are produced in lymphoid tissue where lymph node stromal cells (LNSCs) regulate lymphocyte function. Infections can alter the interaction between LNSCs and lymphocytes resulting in defective immune responses. In rheumatoid arthritis (RA) autoantibody production precedes clinical disease allowing identification of at risk individuals. We investigated the ability of human LNSCs derived from RA, RA-risk and healthy individuals to sense and respond to pathogens. Human LNSCs cultured directly from freshly collected lymph node biopsies expressed TLR1-9 with exception of TLR7. In all donors TLR3 triggering induced expression of ISGs, IL-6 and adhesion molecules like VCAM-1 and ICAM-1. Strikingly, T cell guiding chemokines CCL19 and IL-8 as well as the antiviral gene MxA were less induced upon TLR3 triggering in autoimmune LNSCs. This observed decrease, found already in LNSCs of RA-risk individuals, may lead to incorrect positioning of lymphocytes and aberrant immune responses during viral infections.
An MCEM Framework for Drug Safety Signal Detection and Combination from Heterogeneous Real World Evidence
Authors: Xiao Cao , Li Ying , Baytas Inci M. , Zhou Jiayu , Wang Fei 
Published: January 2018

AbstractDelayed drug safety insights can impact patients, pharmaceutical companies, and the whole society. Post-market drug safety surveillance plays a critical role in providing drug safety insights, where real world evidence such as spontaneous reporting systems (SRS) and a series of disproportional analysis serve as a cornerstone of proactive and predictive drug safety surveillance. However, they still face several challenges including concomitant drugs confounders, rare adverse drug reaction (ADR) detection, data bias, and the under-reporting issue. In this paper, we are developing a new framework that detects improved drug safety signals from multiple data sources via Monte Carlo Expectation-Maximization (MCEM) and signal combination. In MCEM procedure, we propose a new sampling approach to generate more accurate SRS signals for each ADR through iteratively down-weighting their associations with irrelevant drugs in case reports. While in signal combination step, we adopt Bayesian hierarchical model and propose a new summary statistic such that SRS signals can be combined with signals derived from other observational health data allowing for related signals to borrow statistical support with adjustment of data reliability. They combined effectively alleviate the concomitant confounders, data bias, rare ADR and under-reporting issues. Experimental results demonstrated the effectiveness and usefulness of the proposed framework.
Multiplane and Spectrally-Resolved Single Molecule Localization Microscopy with Industrial Grade CMOS cameras
Authors: Babcock Hazen P. 
Published: January 2018

AbstractThis work explores the use of industrial grade CMOS cameras for single molecule localization microscopy (SMLM). We show that industrial grade CMOS cameras approach the performance of scientific grade CMOS cameras at a fraction of the cost. This makes it more economically feasible to construct high-performance imaging systems with multiple cameras that are capable of a diversity of applications. In particular we demonstrate the use of industrial CMOS cameras for biplane, multiplane and spectrally resolved SMLM. We also provide open-source software for simultaneous control of multiple CMOS cameras and for the reduction of the movies that are acquired to super-resolution images.
PlanHab Study: Consequences of combined normobaric hypoxia and bed rest on adenosine kinetics
Authors: Strewe C. , Zeller R. , Feuerecker M. , Hoerl M. , Matzel S. , Kumprej I. , Crispin A. , Johannes B. , Debevec T. , Mekjavic I. B. , Eiken O. , Thiel M. , Schelling G. , Choukèr A. 
Published: January 2018

AbstractAdenosine plays a role in the energy supply of cells and provokes differential, hormone-like functions in circulating cells and various tissues. Its release is importantly regulated by oxygen tension. This renders adenosine and its kinetics interesting to investigate in humans subjected to low oxygen conditions. Especially for space exploration scenarios, hypoxic conditions – together with reduced gravity - represent two foreseen living conditions when planning manned long-duration space missions or planetary habitats. The PlanHab study investigated microgravity through inactivity in bed rest and normobaric hypoxia to examine their independent or combined effect on adenosine and its kinetics. Healthy male subjects (n = 14) completed three 21-day interventions: hypoxic bed rest (HBR); hypoxic ambulatory confinement (HAMB); normoxic bed rest (NBR). The interventions were separated by 4 months. Our hypothesis of a hypoxia-triggered increase in adenosine was confirmed in HAMB but unexpectedly also in NBR. However, the highest adenosine levels were noted following HBR. Furthermore, the percentage of hemolysis was elevated in HBR whereas endothelial integrity markers stayed low in all three interventions. In summary, these data suggest that neocytolysis accounts for these effects while we could reduce evidence for microcirculatory changes.
A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection
Authors: Pan Jian-Zhang , Fang Pan , Fang Xiao-Xia , Hu Ting-Ting , Fang Jin , Fang Qun 
Published: January 2018

AbstractIn this work, we developed a miniaturized palmtop high-speed capillary electrophoresis (CE) system integrating whole modules, including picoliter-scale sample injection, short capillary-based fast CE, high-voltage power supply, orthogonal laser induced fluorescence (LIF) detection, battery, system control, on-line data acquisition, processing, storage, and display modules. A strategy of minimalist miniaturization combining minimal system design and low-cost system construction was adopted to achieve the instrument miniaturization with extremely low cost, which is differing from the current microfabrication strategy used in most reported miniaturized CE systems. With such a strategy, the total size of the bioanalyzer was minimized to 90 × 75 × 77 mm (length × width × height) and the instrument cost was reduced to ca. $500, which demonstrated the smallest and lowest-cost CE instrument with LIF detection in so far reported systems. The present bioanalyzer also exhibited comparable analytical performances to previously-reported high-speed CE systems. A limit of detection of 1.02 nM sodium fluorescein was obtained. Fast separations were achieved for multiple types of samples as amino acids, amino acid enantiomers, DNA fragments, and proteins with high efficiency. We applied this instrument in colorectal cancer diagnosis for detecting KRAS mutation status by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Comparison of focused cognitive training and portable “brain-games” on functional outcomes for vocational rehabilitation participants
Authors: Bell Morris D. , Laws Holly , Pittman Brian , Johannesen Jason K. 
Published: January 2018

AbstractCognitive remediation performed in a cognitive laboratory was compared with a sham control using portable brain games to study effects on vocational, neurocognitive, and functional outcomes for participants with psychotic disorders in vocational rehabilitation (VR). Seventy-seven participants (61% schizophrenia, 39% other psychosis) in transitional (45.5%) or supported employment (54.5%) were randomly assigned to 6 months of portable cognitive-games (CG) or cognitive remediation (CR) plus a weekly goal-setting group, and evaluated during training, post-training and at 12 months. Overall rates of employment did not differ significantly at 12-month follow-up; however, VR + CG attained employment more rapidly during training. A significant time by condition interaction favored VR + CR on Quality of Life Total Score and Instrumental Functioning over 12 months. Neurocognitive outcomes favored VR + CR, particularly on attention. Training hours related significantly to neurocognitive improvement regardless of condition. No differences were found in training adherence despite portability for VR + CG. Results indicate that VR + CR had significantly greater effect than VR + CG on neurocognition and community functioning, but not on employment outcome. Job attainment rates during the training period revealed a potential advantage for portable training raising new questions concerning how cognitive remediation can be most effectively integrated with VR.
Phase Coexistence and Kinetic Arrest in the Magnetostructural Transition of the Ordered Alloy FeRh
Authors: Keavney David J. , Choi Yongseong , Holt Martin V. , Uhlíř Vojtěch , Arena Dario , Fullerton Eric E. , Ryan Philip J. , Kim Jong-Woo 
Published: January 2018

AbstractIn materials where two or more ordering degrees of freedom are closely matched in their free energies, coupling between them, or multiferroic behavior can occur. These phenomena can produce a very rich phase behavior, as well as emergent phases that offer useful properties and opportunities to reveal novel phenomena in phase transitions. The ordered alloy FeRh undergoes an antiferromagnetic to ferromagnetic phase transition at ~375 K, which illustrates the interplay between structural and magnetic order mediated by a delicate energy balance between two configurations. We have examined this transition using a combination of high-resolution x-ray structural and magnetic imaging and comprehensive x-ray magnetic circular dichroism spectroscopy. We find that the transition proceeds via a defect-driven domain nucleation and growth mechanism, with significant return point memory in both the structural and magnetic domain configurations. The domains show evidence of inhibited growth after nucleation, resulting in a quasi-2nd order temperature behavior.
Edaphic characterization and plant zonation in the Qaidam Basin, Tibetan Plateau
Authors: Wang Xianjie , Kong Fanjing , Kong Weigang , Xu Wenning 
Published: January 2018

AbstractThis paper presents a study of edaphic characteristics and their relationship with plant distribution in the Qaidam Basin, Tibetan Plateau, and establishes a distribution model for plants in sandy gravel Gobi to dry salt lake areas. All of the communities in the study area were dominated by plants with strong saline-alkaline tolerance. In this area, salts appeared to migrate to the surface; the surface soil was striped, and the salt distribution varied from sandy gravel Gobi to dry salt lake areas. The salt composition mainly consisted of NaCl in the surface crust. In the subsurface layers, the salt composition was dominated by Ca2+, Cl− and SO42−. The type of vegetation at the study site can be divided into two categories: salt-tolerant vegetation and weakly salt-tolerant vegetation. The salt-tolerant vegetation is influenced by Na+, Cl−, and the salinity. The soil of these vegetation communities had a higher salt and Na+ concentration and a lower Ca2+ and K+ concentration. The weakly salt-tolerant vegetation is mainly affected by the Ca2+/Na+ and K+/Na+ ratios. Based on the above results, a vegetation distribution model for saline lakes on the inland plateau was established.