bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toxicological evaluation of the topoisomerase inhibitor, etoposide, in the model animal Caenorhabditis elegans and 3T3‐L1 normal murine cells
Authors: Lee So Young , Kim Joo Yeon , Jung Yu‐Jin , Kang Kyungsu 
Published: February 2017

AbstractEtoposide, a topoisomerase II inhibitor, has been widely used as a clinical anticancer drug to treat diverse cancer patients. Since not only rapidly dividing cancer cells but also the cells of normal human tissues and every living organism in environmental ecosystems have topoisomerases, it is crucial to study the toxicity of etoposide in other organisms in addition to cancer cells. In this study, we evaluated the toxicity of etoposide in both a soil nematode, Caenorhabditis elegans, and 3T3‐L1 normal murine cells. Etoposide significantly retarded the growth, egg laying, and hatching in C. elegans. Etoposide also affected the reproductive gonad tissue, decreased the number of germ cells and induced abnormally enlarged nuclei in C. elegans. In addition, etoposide inhibited 3T3‐L1 cell proliferation, with IC50 values of 37.8 ± 7.3 and 9.8 ± 1.8 μM after 24 and 48 hours of treatment, respectively, via the induction of cell cycle arrest at the G2/M phase and apoptotic cell death. Etoposide also induced nuclear enlargement in 3T3‐L1 normal murine cells. The reproductive toxicity and abnormal nuclear morphological changes seemed to correlate with the adverse effects of etoposide. We suggest that these experimental platforms, i.e., the toxicological evaluation of both nematodes and 3T3‐L1 cells, may be useful to study the mechanisms underlying the side effects of chemicals, including topoisomerase inhibitors.
Experiences of Pre‐Implantation Genetic Diagnosis (PGD) in Sweden: a Three‐Year Follow‐Up of Men and Women
Authors: Järvholm Stina , Thurin‐Kjellberg Ann , Broberg Malin 
Published: February 2017

AbstractMen and women with a hereditary genetic disease are faced with different options when they wish to become parents. One is pre‐implantation genetic diagnosis (PGD) which is a combination of in vitro fertilization (IVF) and genetic analysis of the embryo before implantation. The present study focused on men and women's psychological experiences of PGD three years after applying for PGD. Nineteen women and seventeen men (i.e. seventeen couples and two women) participated. The interviews were analysed by thematic method. It is better to have tried was identified as a master theme, under which came three underlying sub‐themes, which had the following headings: Practical experience of PGD, Psychological experience of PGD and Goals of PGD. The results show that men and women three years after PGD are still psychologically affected by their experience. The men and women raised concerns that their relationship had been affected, both positively and negatively, and feelings of anxiety and depression still remained. Healthcare services should recognize the heterogeneous nature of the group being studied and therefore the need for counselling can arise at different times and in relation to different areas, regardless of the outcome of the PGD.
Prelates at War: Cardinal Bourne, Bishop Ward and the St. Edmund's College Cadet Corps Dispute
Authors: Foster Stewart 
Published: February 2017

Ernest Oldmeadow, in the second volume of his biography—some have said hagiography—of Cardinal Bourne, begins the chapter in which he deals with the First World War with the following statement:
When the ‘Great’ War broke out, Cardinal Bourne was in a better position than that of some other religious leaders. Many of them, before calling their followers to fight with might and main against the enemy, had to recant or explain away their pacifist pasts. Some of them had pronounced all war unjustifiable, and had obstructed the training of their fatherland's manhood, even for defence. Not so Francis Bourne.
The Making of a Convert: John Henry Newman's Oriel and Littlemore Experience
Authors: Nockles Peter 
Published: February 2017

‘The flood is round thee, but thy towers as yetAre safe, and clear as by a summer’s sea…Lo! On the top of each aerial spireWhat seems a star by day, so high and brightIt quivers from afar in golden light.But ‘tis a form of earth, though touched with fireCelestial, raised in other days, to tellHow, when they tired of prayer, Apostles fell’.John Henry Newman's poem ‘On Oxford’ published within a section called ‘Champions of the Truth’ in the verse collection, Lyra Apostolica, which he edited in 1836, encapsulates Newman's vision of Oxford and its colleges. Oxford was portrayed in the poem as an embattled but triumphant ‘city on a hill’ (in spite of its valley location surrounded by hills); a bulwark against contemporary forces, religious, and political, which for Newman, seemed to threaten it in the 1830s. The poem reminds us that the Oxford Movement, the great movement of religious revival within the Church of England commonly dated from 1833, the movement which Newman famously led and inspired, was rooted in Newman's keen and abiding sense of place (genius loci, as he put it), of memory, tradition, ethos, and association.
Mirage: an O(n) time analytical solution to 3D camera pose estimation with multi-camera support
Authors: Dinc Semih , Fahimi Farbod , Aygun Ramazan 
Published: February 2017

SUMMARYMirage is a camera pose estimation method that analytically solves pose parameters in linear time for multi-camera systems. It utilizes a reference camera pose to calculate the pose by minimizing the 2D projection error between reference and actual pixel coordinates. Previously, Mirage has been successfully applied to trajectory tracking (visual servoing) problem. In this study, a comprehensive evaluation of Mirage is performed by particularly focusing on the area of camera pose estimation. Experiments have been performed using simulated and real data on noisy and noise-free environments. The results are compared with the state-of-the-art techniques. Mirage outperforms other methods by generating fast and accurate results in all tested environments.
Rethinking the Cognitive Mechanisms Underlying Pantomime of Tool Use: Evidence from Alzheimer’s Disease and Semantic Dementia
Authors: Lesourd Mathieu , Baumard Josselin , Jarry Christophe , Etcharry-Bouyx Frédérique , Belliard Serge , Moreaud Olivier , Croisile Bernard , Chauviré Valérie , Granjon Marine , Le Gall Didier , Osiurak François 
Published: February 2017

AbstractObjectives: Pantomiming the use of familiar tools is a central test in the assessment of apraxia. However, surprisingly, the nature of the underlying cognitive mechanisms remains an unresolved issue. The aim of this study is to shed a new light on this issue by exploring the role of functional, mechanical, and manipulation knowledge in patients with Alzheimer’s disease and semantic dementia and apraxia of tool use. Methods: We performed multiple regression analyses with the global performance and the nature of errors (i.e., production and conception) made during a pantomime of tool use task in patients and control participants as dependent variables and tasks investigating functional, mechanical, and manipulation knowledge as predictors. Results: We found that mechanical problem solving, assessing mechanical knowledge, was a good predictor of the global performance of pantomime of tool use. We also found that occurrence of conception errors was robustly predicted by the task assessing functional knowledge whereas that of production errors was not explained by only one predictor. Conclusions: Our results suggest that both functional and mechanical knowledge are important to pantomime the use of tools. To our knowledge, this is the first demonstration that mechanical knowledge plays a role in pantomime of tool use. Although impairment in pantomime of tool use tasks (i.e., apraxia) is widely explained by the disruption of manipulation knowledge, we propose that pantomime of tool use is a complex problem-solving task. (JINS, 2017, 23, 128–138)
Cognitive Control, Learning, and Clinical Motor Ratings Are Most Highly Associated with Basal Ganglia Brain Volumes in the Premanifest Huntington’s Disease Phenotype
Authors: Misiura Maria B. , Lourens Spencer , Calhoun Vince D. , Long Jeffrey , Bockholt Jeremy , Johnson Hans , Zhang Ying , Paulsen Jane S. , Turner Jessica A. , Liu Jingyu , Kara Betul , Fall Elizabeth ,   for the PREDICT-HD Investigators & Working Group
Published: February 2017

AbstractObjectives:Huntington’s disease (HD) is a debilitating genetic disorder characterized by motor, cognitive and psychiatric abnormalities associated with neuropathological decline. HD pathology is the result of an extended chain of CAG (cytosine, adenine, guanine) trinucleotide repetitions in theHTTgene. Clinical diagnosis of HD requires the presence of an otherwise unexplained extrapyramidal movement disorder in a participant at risk for HD. Over the past 15 years, evidence has shown that cognitive, psychiatric, and subtle motor dysfunction is evident decades before traditional motor diagnosis. This study examines the relationships among subcortical brain volumes and measures of emerging disease phenotype in prodromal HD, before clinical diagnosis.Methods:The dataset includes 34 cognitive, motor, psychiatric, and functional variables and five subcortical brain volumes from 984 prodromal HD individuals enrolled in the PREDICT HD study. Using cluster analyses, seven distinct clusters encompassing cognitive, motor, psychiatric, and functional domains were identified. Individual cluster scores were then regressed against the subcortical brain volumetric measurements.Results:Accounting for site and genetic burden (the interaction of age and CAG repeat length) smaller caudate and putamen volumes were related to clusters reflecting motor symptom severity, cognitive control, and verbal learning.Conclusions:Variable reduction of the HD phenotype using cluster analysis revealed biologically related domains of HD and are suitable for future research with this population. Our cognitive control cluster scores show sensitivity to changes in basal ganglia both within and outside the striatum that may not be captured by examining only motor scores. (JINS, 2017,23, 159–170)
Critical Motor Involvement in Prediction of Human and Non-biological Motion Trajectories
Authors: de Wit Matthieu M. , Buxbaum Laurel J. 
Published: February 2017

AbstractObjectives: Adaptive interaction with the environment requires the ability to predict both human and non-biological motion trajectories. Prior accounts of the neurocognitive basis for prediction of these two motion classes may generally be divided into those that posit that non-biological motion trajectories are predicted using the same motor planning and/or simulation mechanisms used for human actions, and those that posit distinct mechanisms for each. Using brain lesion patients and healthy controls, this study examined critical neural substrates and behavioral correlates of human and non-biological motion prediction. Methods: Twenty-seven left hemisphere stroke patients and 13 neurologically intact controls performed a visual occlusion task requiring prediction of pantomimed tool use, real tool use, and non-biological motion videos. Patients were also assessed with measures of motor strength and speed, praxis, and action recognition. Results: Prediction impairment for both human and non-biological motion was associated with limb apraxia and, weakly, with the severity of motor production deficits, but not with action recognition ability. Furthermore, impairment for human and non-biological motion prediction was equivalently associated with lesions in the left inferior parietal cortex, left dorsal frontal cortex, and the left insula. Conclusions: These data suggest that motor planning mechanisms associated with specific loci in the sensorimotor network are critical for prediction of spatiotemporal trajectory information characteristic of both human and non-biological motions. (JINS, 2017, 23, 171–184)
Suicidal ideation and behaviour among community and health care seeking populations in five low- and middle-income countries: a cross-sectional study
Authors: Jordans M. , Rathod S. , Fekadu A. , Medhin G. , Kigozi F. , Kohrt B. , Luitel N. , Petersen I. , Shidhaye R. , Ssebunnya J. , Patel V. , Lund C. 
Published: February 2017

AimsSuicidal behaviour is an under-reported and hidden cause of death in most low- and middle-income countries (LMIC) due to lack of national systematic reporting for cause-specific mortality, high levels of stigma and religious or cultural sanctions. The lack of information on non-fatal suicidal behaviour (ideation, plans and attempts) in LMIC is a major barrier to design and implementation of prevention strategies. This study aims to determine the prevalence of non-fatal suicidal behaviour within community- and health facility-based populations in LMIC.MethodsTwelve-month prevalence of suicidal ideation, plans and attempts were established through community samples (n = 6689) and primary care attendees (n = 6470) from districts in Ethiopia, Uganda, South Africa, India and Nepal using the Composite International Diagnostic Interview suicidality module. Participants were also screened for depression and alcohol use disorder.ResultsWe found that one out of ten persons (10.3%) presenting at primary care facilities reported suicidal ideation within the past year, and 1 out of 45 (2.2%) reported attempting suicide in the same period. The range of suicidal ideation was 3.5–11.1% in community samples and 5.0–14.8% in health facility samples. A higher proportion of facility attendees reported suicidal ideation than community residents (10.3 and 8.1%, respectively). Adults in the South African facilities were most likely to endorse suicidal ideation (14.8%), planning (9.5%) and attempts (7.4%). Risk profiles associated with suicidal behaviour (i.e. being female, younger age, current mental disorders and lower educational and economic status) were highly consistent across countries.ConclusionThe high prevalence of suicidal ideation in primary care points towards important opportunities to implement suicide risk reduction initiatives. Evidence-supported strategies including screening and treatment of depression in primary care can be implemented through the World Health Organization's mental health Global Action Programme suicide prevention and depression treatment guidelines. Suicidal ideation and behaviours in the community sample will require detection strategies to identify at risks persons not presenting to health facilities.
The OncoPPi network of cancer-focused protein–protein interactions to inform biological insights and therapeutic strategies
Authors: Li Zenggang , Ivanov Andrei A. , Su Rina , Gonzalez-Pecchi Valentina , Qi Qi , Liu Songlin , Webber Philip , McMillan Elizabeth , Rusnak Lauren , Pham Cau , Chen Xiaoqian , Mo Xiulei , Revennaugh Brian , Zhou Wei , Marcus Adam , Harati Sahar , Chen Xiang , Johns Margaret A. , White Michael A. , Moreno Carlos S. , Cooper Lee A. D. , Du Yuhong , Khuri Fadlo R. , Fu Haian 
Published: February 2017

AbstractAs genomics advances reveal the cancer gene landscape, a daunting task is to understand how these genes contribute to dysregulated oncogenic pathways. Integration of cancer genes into networks offers opportunities to reveal protein–protein interactions (PPIs) with functional and therapeutic significance. Here, we report the generation of a cancer-focused PPI network, termed OncoPPi, and identification of >260 cancer-associated PPIs not in other large-scale interactomes. PPI hubs reveal new regulatory mechanisms for cancer genes like MYC, STK11, RASSF1 and CDK4. As example, the NSD3 (WHSC1L1)–MYC interaction suggests a new mechanism for NSD3/BRD4 chromatin complex regulation of MYC-driven tumours. Association of undruggable tumour suppressors with drug targets informs therapeutic options. Based on OncoPPi-derived STK11-CDK4 connectivity, we observe enhanced sensitivity of STK11-silenced lung cancer cells to the FDA-approved CDK4 inhibitor palbociclib. OncoPPi is a focused PPI resource that links cancer genes into a signalling network for discovery of PPI targets and network-implicated tumour vulnerabilities for therapeutic interrogation.
Nickel biopathways in tropical nickel hyperaccumulating trees from Sabah (Malaysia)
Authors: van der Ent Antony , Callahan Damien L. , Noller Barry N. , Mesjasz-Przybylowicz Jolanta , Przybylowicz Wojciech J. , Barnabas Alban , Harris Hugh H. 
Published: February 2017

AbstractThe extraordinary level of accumulation of nickel (Ni) in hyperaccumulator plants is a consequence of specific metal sequestering and transport mechanisms, and knowledge of these processes is critical for advancing an understanding of transition element metabolic regulation in these plants. The Ni biopathways were elucidated in three plant species, Phyllanthus balgooyi, Phyllanthus securinegioides (Phyllanthaceae) and Rinorea bengalensis (Violaceae), that occur in Sabah (Malaysia) on the Island of Borneo. This study showed that Ni is mainly concentrated in the phloem in roots and stems (up to 16.9% Ni in phloem sap in Phyllanthus balgooyi) in all three species. However, the species differ in their leaves – in P. balgooyi the highest Ni concentration is in the phloem, but in P. securinegioides and R. bengalensis in the epidermis and in the spongy mesophyll (R. bengalensis). The chemical speciation of Ni2+ does not substantially differ between the species nor between the plant tissues and transport fluids, and is unambiguously associated with citrate. This study combines ion microbeam (PIXE and RBS) and metabolomics techniques (GC-MS, LC-MS) with synchrotron methods (XAS) to overcome the drawbacks of the individual techniques to quantitatively determine Ni distribution and Ni2+ chemical speciation in hyperaccumulator plants.
TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway
Authors: Xu Guoxing , Guo Yongfei , Xu Dabo , Wang Yi , Shen Yafeng , Wang Feifei , Lv Yuanyuan , Song Fanglong , Jiang Dawei , Zhang Yinquan , Lou Yi , Meng Yake , Yang Yongji , Kang Yifan 
Published: February 2017

AbstractRecent studies have shown that some members of the tripartite motif-containing protein (TRIM) family serve as important regulators of tumorigenesis. However, the biological role of TRIM14 in osteosarcoma remains to be established. In this study, we showed that TRIM14 is upregulated in human osteosarcoma specimens and cell lines, and correlated with osteosarcoma progression and shorter patient survival times. Functional studies demonstrated that overexpression of TRIM14 enhances osteosarcoma cell proliferation, clone formation, cell cycle procession, migration and invasion in vitro and promotes tumor growth in vivo, and conversely, its silencing has the opposite effects. Furthermore, TRIM14 overexpression induced activation of the AKT pathway. Inhibition of AKT expression reversed the TRIM14-mediated promotory effects on cell growth and mobility, in addition to TRIM14-induced epithelial-to-mesenchymal transition (EMT) and cyclin D1 upregulation. Our findings collectively suggest that TRIM14 functions as an oncogene by upregulating the AKT signaling pathway in osteosarcoma cells, supporting its potential utility as a therapeutic target for this disease.
Genetic polymorphism and evolutionary differentiation of Eastern Chinese Han: a comprehensive and comparative analysis on KIRs
Authors: Yin Caiyong , Hu Li , Huang Huijie , Yu Yanfang , Li Zheng , Ji Qiang , Kong Xiaochao , Wang Zhongqun , Yan Jinchuan , Yan Jiangwei , Zhu Bofeng , Chen Feng 
Published: February 2017

AbstractKiller cell immunoglobulin-like receptor genes, namely KIRs, cluster together within the 160 kb genomic DNA region. In this study, we used PCR-SSP approach and successfully identified the genotype of 17 KIR genes in 123 independent healthy donors residing in the Jiangsu province, China. All individuals were positive at the 7 genes. The observed carrier gene frequencies (OFs) of remaining 10 KIRs ranged from 14.63% (KIR2DS3) to 95.93% (KIR3DL1). We found 27 distinct genotypes excluding KIR1D. The most frequent occurred in 63 individuals (51.22%). The linkage disequilibrium analysis signified 29 positive and 6 negative relations in 45 pairwise comparisons. To study population differentiation, we drew a Heatmap based on the data of KIRs from 59 populations and conducted Hierarchical Clustering by Euclidean distances. We next validated our results by estimating pairwise DA distances and illustrating a Neighbor-Joining tree, as well as a MDS plot covering 3 additional Chinese Han groups. The phylogenetic reconstruction and cluster analysis strongly indicated a genetically close relationship between Eastern and Jilin Hans. In conclusion, the present study provided a meritorious resource of KIR genotyping for population genetics, and could be helpful to uncover the genetic mechanism of KIRs in immune disease in the future.
An empirical analysis of the Ebola outbreak in West Africa
Authors: Khaleque Abdul , Sen Parongama 
Published: February 2017

AbstractThe data for the Ebola outbreak that occurred in 2014–2016 in three countries of West Africa are analysed within a common framework. The analysis is made using the results of an agent based Susceptible-Infected-Removed (SIR) model on a Euclidean network, where nodes at a distance l are connected with probability P(l) ∝ l−δ, δ determining the range of the interaction, in addition to nearest neighbors. The cumulative (total) density of infected population here has the form "Equation missing", where the parameters depend on δ and the infection probability q. This form is seen to fit well with the data. Using the best fitting parameters, the time at which the peak is reached is estimated and is shown to be consistent with the data. We also show that in the Euclidean model, one can choose δ and q values which reproduce the data for the three countries qualitatively. These choices are correlated with population density, control schemes and other factors. Comparing the real data and the results from the model one can also estimate the size of the actual population susceptible to the disease. Rescaling the real data a reasonably good quantitative agreement with the simulation results is obtained.
CR-LAAO, an L-amino acid oxidase from Calloselasma rhodostoma venom, as a potential tool for developing novel immunotherapeutic strategies against cancer
Authors: Costa Tássia R. , Menaldo Danilo L. , Zoccal Karina F. , Burin Sandra M. , Aissa Alexandre F. , Castro Fabíola A. de , Faccioli Lúcia H. , Greggi Antunes Lusânia M. , Sampaio Suely V. 
Published: February 2017

AbstractL-amino acid oxidases from snake venoms have been described to possess various biological functions. In this study, we investigated the inflammatory responses induced in vivo and in vitro by CR-LAAO, an L-amino acid oxidase isolated from Calloselasma rhodostoma venom, and its antitumor potential. CR-LAAO induced acute inflammatory responses in vivo, with recruitment of neutrophils and release of IL-6, IL-1β, LTB4 and PGE2. In vitro, IL-6 and IL-1β production by peritoneal macrophages stimulated with CR-LAAO was dependent of the activation of the Toll-like receptors TLR2 and TLR4. In addition, CR-LAAO promoted apoptosis of HL-60 and HepG2 tumor cells mediated by the release of hydrogen peroxide and activation of immune cells, resulting in oxidative stress and production of IL-6 and IL-1β that triggered a series of events, such as activation of caspase 8, 9 and 3, and the expression of the pro-apoptotic gene BAX. We also observed that CR-LAAO modulated the cell cycle of these tumor cells, promoting delay in the G0/G1 and S phases. Taken together, our results suggest that CR-LAAO could serve as a potential tool for the development of novel immunotherapeutic strategies against cancer, since this toxin promoted apoptosis of tumor cells and also activated immune cells against them.