bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trepanation in Italy: A Review
Authors: Giuffra V. , Fornaciari G. 
Published: March 2017

AbstractThis paper reviews and discusses cases of skull trepanation in ancient Italy on the basis of information provided by the literature. A total of 54 individuals from 43 different Italian archaeological sites were found to have evidence of trepanation. The analysis of evidences of trepanation in Italy has demonstrated that no differences can generally be perceived between the trepanned individual and the social context of the burial, leading to exclude a special role of the former within the group. Trepanation in Italy covers a time span of approximately 7000 years, the most ancient cases dating back to the fifth millennium bc and the most recent to the 18th–19th centuries ad. The geographic distribution appears quite homogeneous, with a prevalence of cases in Central Italy and abundant evidence from prehistoric Sardinia. The majority of individuals show a single trepanation, whereas others present skulls with multiple holes. Trepanation in Italy was reserved to adult individuals, except for some rare cases, and shows a relevant preponderance for the male sex. The most diffused technique is scraping; cutting and drilling are less attested, especially as unique techniques for trepanation, but they were used more frequently in combination with scraping. Trepanation could in some cases be hypothesised as therapeutic intervention for the treatment of a traumatic wound or of other pathologies, whereas in other cases, a number of evidences are attested of trepanation performed as probable ritual intervention or as experimental surgery; in the remaining cases, the reasons for trepanation are unclear or not determinable. A high percentage of long‐term healing associated with trepanation has been evidenced. Copyright © 2017 John Wiley & Sons, Ltd.
Multifunctional nanoparticle composites: progress in the use of soft and hard nanoparticles for drug delivery and imaging
Authors: Sangtani Ajmeeta , Nag Okhil K. , Field Lauren D. , Breger Joyce C. , Delehanty James B. 
Published: March 2017

With continued advancements in nanoparticle (NP) synthesis and in the interfacing of NPs with biological systems has come the exponential growth in the use of NPs for therapeutic drug delivery and imaging applications. In recent years, the advent of NP multifunctionality—the ability to perform multiple, disparate functions on a single NP platform—has garnered much excitement for the potential realization of highly functional NP‐mediated drug delivery for use in the clinical setting. This Overview will survey the current state of the art (reports published within the last 5 years) of multifunctional NPs for therapeutic drug delivery, imaging or a combination thereof. We provide extensive examples of both soft (micelles, liposomes, polymeric NPs) and hard (noble metals, quantum dots, metal oxides) NP formulations that have been used for multimodal drug delivery and imaging. The criteria for inclusion, herein, is that there must be at least two therapeutic drug cargos or imaging agents or a combination of the two. We next offer an assessment of the cytotoxicity of therapeutic NP constructs in biological systems. We then conclude with a forward‐looking perspective on how we expect this field to develop in the coming years. WIREs Nanomed Nanobiotechnol 2017, 9:e1466. doi: 10.1002/wnan.1466This article is categorized under:

Diagnostic Tools > Diagnostic Nanodevices
Diagnostic Tools > In Vitro Nanoparticle-Based Sensing
Diagnostic Tools > In Vivo Nanodiagnostics and Imaging
Redirecting carbon flux throughpgi-deficient and heterologous transhydrogenase toward efficient succinate production inCorynebacterium glutamicum
Authors: Wang Chen , Zhou Zhihui , Cai Heng , Chen Zhongjun , Xu Hongtao 
Published: March 2017

AbstractCorynebacterium glutamicum is particularly known for its potentiality in succinate production. We engineered C. glutamicum for the production of succinate. To enhance C3–C4 carboxylation efficiency, chromosomal integration of the pyruvate carboxylase gene pyc resulted in strain NC-4. To increase intracellular NADH pools, the pntAB gene from Escherichia coli, encoding for transhydrogenase, was chromosomally integrated into NC-4, leading to strain NC-5. Furthermore, we deleted pgi gene in strain NC-5 to redirect carbon flux to the pentose phosphate pathway (PPP). To solve the drastic reduction of PTS-mediated glucose uptake, the ptsG gene from C. glutamicum, encoding for the glucose-specific transporter, was chromosomally integrated into pgi-deficient strain resulted in strain NC-6. In anaerobic batch fermentation, the production of succinate in pntAB-overexpressing strain NC-5 increased by 14% and a product yield of 1.22 mol/mol was obtained. In anaerobic fed-batch process, succinic acid concentration reached 856 mM by NC-6. The yields of succinate from glucose were 1.37 mol/mol accompanied by a very low level of by-products. Activating PPP and transhydrogenase in combination led to a succinate yield of 1.37 mol/mol, suggesting that they exhibited a synergistic effect for improving succinate yield.
Post-Dural Puncture Headache is Uncommon in Young Ambulatory Surgery Patients
Authors: DelPizzo Kathryn , Cheng Jennifer , Dong Naomi , Edmonds Chris R. , Kahn Richard L. , Fields Kara G. , Curren Jodie , Rotundo Valeria , Zayas Victor M. 
Published: March 2017

Background The incidence of post-dural puncture headache (PDPH) arising from spinal anesthesia in the general population is low. However, patients under 45 years have been shown to exhibit a higher incidence of PDPH, even with small needles. Questions/Purposes This study aimed to estimate the incidence of PDPH from a 27G pencil-point needle in ambulatory surgery patients between the ages of 15–45 years and compare incidence of PDPH by age group, sex, and history of headache. Methods In this prospective cohort study, 300 patients (15–45 years old) who underwent simple knee arthroscopy under spinal anesthesia with a 27G pencil-point needle were enrolled. Verbal consent was obtained during the initial phone conversation between post-operative days (PODs) 2–5. A PDPH questionnaire was administered during this conversation and between PODs 7–10. Patients who reported a positional headache were contacted by a physician co-investigator, who determined PDPH diagnosis. Results Five patients were excluded from analysis due to complicated operative procedures or spinal needle size. The overall PDPH incidence was 2.0% (95% CI 0.9–4.4; 6/295). PDPH incidence in 15–19-year-old patients was 16.7% (95% CI 4.7–44.8; 2/12). The crude relative risk of PDPH was 15.4 (95% CI 2.8–114.4) for patients with and without history of headache and 2.5 (95% CI 0.5–14.8) for females vs. males. Overall, 16.3% (95% CI 12.5–20.9) of patients reported post-operative, non-positional headaches. Conclusions There was a low overall incidence of PDPH among patients aged 15–45. Pre-planned subgroup analyses of PDPH incidence by age group revealed a high risk of PDPH among a small sample of 15–19-year-olds.
Massive Formation of Equiaxed Crystals by Avalanches of Mushy Zone Segments
Authors: Ludwig A. , Stefan-Kharicha M. , Kharicha A. , Wu M. 
Published: March 2017

Abstract
It is well known that the growth and motion of equiaxed crystals govern important microstructural features, especially in larger castings such as heavy ingots. To determine the origin of the equiaxed crystals, heterogeneous nucleation, and/or fragmentation of dendrite arms from columnar regions are often discussed. In the present study, we demonstrate that under certain conditions relatively large areas of mushy regions slide downward and form spectacular crystal avalanches. These avalanches crumble into thousands of dendritic fragments, whereby the larger fragments immediately sediment and the smaller proceed to behave as equiaxed crystals. Traces of such crystal avalanches can be seen by conspicuous equiaxed layers in the lower part of the casting. From the arguments in the discussion, it is believed that such a phenomenon may occur in alloys which reveal an upward solutal buoyancy in the interdendritic mush. This would include certain steels and other alloys such as Cu-Al, Pb-Sn, or Ni-Al-alloys. Moreover, the occurrence of crystal avalanches contribute to the formation of V-segregations.
Evaluation of Olive Preservation Methods on Bioactive Constituents and Antioxidant Properties of Olive Oils
Authors: Irmak Şahnur , Kadiroğlu Pınar , Ötleş Semih 
Published: March 2017

AbstractThe purpose of this study was to determine the effects of preservation methods on less salty natural black table olives by evaluating the extracted olive oils. For this purpose, olives of the Gemlik variety obtained in two harvest years were brined and dry salted with 2 and 4% levels of salt and packaged with vacuum and modified atmosphere (60% N2 and 40% CO2) and treated with gamma irradiation (0, 1, 3, 5 kGy). The olive oil extraction process was applied to raw olives, olives after fermentation and olives at 4th and 8th months of storage time. Bioactive component analyses including total carotenoid, total chlorophyll, α‐tocopherol analyses and antioxidant properties were performed along with determination of free fatty acids content (FFA) and peroxide values (PV). FFA and PV increased while α‐tocopherol (TO), total chlorophyll (TCH), total carotenoids (TC) and antioxidant activity (DPPH· radical scavenging assay) values decreased with storage and increment of irradiation dosage. Principal components analyses (PCA) showed that olive oils were discriminated clearly according to harvest year, salt content and processing type (dry‐salting and brining). Overall, this study revealed that chemical parameters of olive oils as the main component of the olives were affected by the harvest year, processing and preservation conditions along with fermentation and storage period of olives.
FINDING ‘ARATUS’: PHAENOMENA 367–85 AND LEONIDAS, ANTH. PAL. 9.25
Authors: Campbell Charles S. , Ryan John J. 
Published: March 2017

Aratus’ Phaenomena calls upon its reader to scrutinize the letters of the text as carefully as the stars and constellations that form its subject matter. The poem abounds with clever letter-play and wordplay, and its reception too is characterized by verbal cleverness, as later authors vie with Aratus and one another to create ingenious textual effects. Among the best-known examples is the word ἄρρητον (‘unspoken’) at Phaen. 2, a witty hidden sphragis for Aratus, who nowhere in his work directly names himself. Later poets picked up on this pun: Callimachus speaks of the λεπταὶ ῥήσιες Ἀρήτου (Anth. Pal. 9.507.3-4 = 27 Pf.), repeating the verbal root of ἄρρητον in ῥήσιες and its sound in Ἀρήτου. Leonidas of Tarentum meanwhile rates Aratus ‘second after Zeus’ (Διὸς … δεύτερος, Anth. Pal. 9.25.5-6), acknowledging the hidden sphragis, which follows directly after the invocation of Zeus in line 1 of the Phaenomena.
Atom Probe Tomographic Characterization of Nanoscale Cu-Rich Precipitates in 17-4 Precipitate Hardened Stainless Steel Tempered at Different Temperatures
Authors: Wang Zemin , Fang Xulei , Li Hui , Liu Wenqing 
Published: March 2017

AbstractThe formation of copper-rich precipitates of 17-4 precipitate hardened stainless steel has been investigated, after tempering at 350–570°C for 4 h, by atom probe tomography (APT). The results reveal that the clusters, enriched only with Cu, were observed after tempering at 420°C. Segregation of Ni, Mn to the Cu-rich clusters took place at 450°C, contributing to the increased hardening. After tempering at 510°C, Ni and Mn were rejected from Cu-rich precipitates and accumulated at the precipitate/matrix interfaces. Al and Si were present and uniformly distributed in the precipitates that were <1.5 nm in radius, but Ni, Mn, Al, and Si were enriched at the interfaces of larger precipitates/matrix. The proxigram profiles of the Cu-rich precipitates formed at 570°C indicated that Ni, Mn, Al, and Si segregated to the precipitate/matrix interfaces to form a Ni(Fe, Mn, Si, Al) shell, which significantly reduced the interfacial energy as the precipitates grew into an elongated shape. In addition, the number density of Cu-rich precipitates was increased with the temperature elevated from 350 up to 450°C and subsequently decreased at higher temperatures. Also, the composition of the matrix and the precipitates were measured and found to vary with temperature.
Cystatin SN inhibits auranofin-induced cell death by autophagic induction and ROS regulation via glutathione reductase activity in colorectal cancer
Authors: Oh Byung Moo , Lee Seon-Jin , Cho Hee Jun , Park Yun Sun , Kim Jong-Tae , Yoon Suk Ran , Lee Sang Chul , Lim Jong-Seok , Kim Bo-Yeon , Choe Yong-Kyung , Lee Hee Gu 
Published: March 2017

AbstractCystatin SN (CST1) is a specific inhibitor belonging to the cystatin superfamily that controls the proteolytic activities of cysteine proteases such as cathepsins. Our previous study showed that high CST1 expression enhances tumor metastasis and invasiveness in colorectal cancer. Recently, auranofin (AF), a gold(I)-containing thioredoxin reductase 1 (TrxR1) inhibitor, has been used clinically to treat rheumatoid arthritis. AF is a proteasome-associated deubiquitinase inhibitor and can act as an anti-tumor agent. In this study, we investigated whether CST1 expression induces autophagy and tumor cell survival. We also investigated the therapeutic effects of AF as an anti-tumor agent in colorectal cancer (CRC) cells. We found that CRC cells expressing high levels of CST1 undergo increased autophagy and exhibit chemotherapeutic resistance to AF-induced cell death, while those expressing low levels of CST1 are sensitive to AF. We also observed that knockdown of CST1 in high-CST1 CRC cells using CST1-specific small interfering RNAs attenuated autophagic activation and restored AF-induced cell mortality. Conversely, the overexpression of CST1 increased autophagy and viability in cells expressing low levels of CST1. Interestingly, high expression of CST1 attenuates AF-induced cell death by inhibiting intracellular reactive oxygen species (ROS) generation, as demonstrated by the fact that the blockage of ROS production reversed AF-induced cell death in CRC cells. In addition, upregulation of CST1 expression increased cellular glutathione reductase (GR) activity, reducing the cellular redox state and inducing autophagy in AF-treated CRC cells. These results suggest that high CST1 expression may be involved in autophagic induction and protects from AF-induced cell death by inhibition of ROS generation through the regulation of GR activity.
Astrocyte hepcidin is a key factor in LPS-induced neuronal apoptosis
Authors: You Lin-Hao , Yan Cai-Zhen , Zheng Bing-Jie , Ci Yun-Zhe , Chang Shi-Yang , Yu Peng , Gao Guo-Fen , Li Hai-Yan , Dong Tian-Yu , Chang Yan-Zhong 
Published: March 2017

AbstractInflammatory responses involving microglia and astrocytes contribute to the pathogenesis of neurodegenerative diseases (NDs). In addition, inflammation is tightly linked to iron metabolism dysregulation. However, it is not clear whether the brain inflammation-induced iron metabolism dysregulation contributes to the NDs pathogenesis. Herein, we demonstrate that the expression of the systemic iron regulatory hormone, hepcidin, is induced by lipopolysaccharide (LPS) through the IL-6/STAT3 pathway in the cortex and hippocampus. In this paradigm, activated glial cells are the source of IL-6, which was essential in the iron overload-activated apoptosis of neurons. Disrupting astrocyte hepcidin expression prevented the apoptosis of neurons, which were able to maintain levels of FPN1 adequate to avoid iron accumulation. Together, our data are consistent with a model whereby inflammation initiates an intercellular signaling cascade in which activated microglia, through IL-6 signaling, stimulate astrocytes to release hepcidin which, in turn, signals to neurons, via hepcidin, to prevent their iron release. Such a pathway is relevant to NDs in that it links inflammation, microglia and astrocytes to neuronal damage.
A high-salt diet enhances leukocyte adhesion in association with kidney injury in young dahl salt-sensitive rats
Authors: Takahashi Hidenori , Nakagawa Suguru , Wu Yaqiong , Kawabata Yukari , Numabe Atsushi , Yanagi Yasuo , Tamaki Yasuhiro , Uehara Yoshio , Araie Makoto 
Published: March 2017

AbstractSalt-sensitive hypertension is associated with severe organ damage. Generating oxygen radicals is an integral component of salt-induced kidney damage, and activated leukocytes are important in oxygen radical biosynthesis. We hypothesized that a high-salt diet causes the upregulation of immune-related mechanisms, thereby contributing to the susceptibility of Dahl salt-sensitive rats to hypertensive kidney damage. For verifying the hypothesis, we investigated leukocytes adhering to retinal vessels when Dahl salt-sensitive rats were challenged with a high-salt (8% NaCl) diet using acridine orange fluoroscopy and a scanning laser ophthalmoscope. The high-salt diet increased leukocyte adhesion after 3 days and was associated with a significant increase in mRNA biosynthesis of monocyte chemotactic protein-1 and intercellular adhesion molecule-1 (ICAM-1) -related molecules in the kidney. Losartan treatment did not affect increased leukocyte adhesion during the early, pre-hypertensive phase of high salt loading; however, losartan attenuated the adhesion of leukocytes during the hypertensive stage. Moreover, the inhibition of leukocyte adhesion in the pre-hypertensive stage by anti-CD18 antibodies decreased tethering of leukocytes and was associated with the attenuation of functional and morphological kidney damage without affecting blood pressure elevation. In conclusion, a high-salt challenge rapidly increased leukocyte adhesion through the over-expression of ICAM-1. Increased leukocyte adhesion in the pre-hypertensive stage is responsible for subsequent kidney damage in Dahl salt-sensitive rats. Immune system involvement may be a key component that initiates kidney damage in a genetic model of salt-induced hypertension.
A human antibody against Zika virus crosslinks the E protein to prevent infection
Authors: Hasan S. Saif , Miller Andrew , Sapparapu Gopal , Fernandez Estefania , Klose Thomas , Long Feng , Fokine Andrei , Porta Jason C. , Jiang Wen , Diamond Michael S. , Crowe James E. , Kuhn Richard J. , Rossmann Michael G. 
Published: March 2017

AbstractThe recent Zika virus (ZIKV) epidemic has been linked to unusual and severe clinical manifestations including microcephaly in fetuses of infected pregnant women and Guillian-Barré syndrome in adults. Neutralizing antibodies present a possible therapeutic approach to prevent and control ZIKV infection. Here we present a 6.2 Å resolution three-dimensional cryo-electron microscopy (cryoEM) structure of an infectious ZIKV (strain H/PF/2013, French Polynesia) in complex with the Fab fragment of a highly therapeutic and neutralizing human monoclonal antibody, ZIKV-117. The antibody had been shown to prevent fetal infection and demise in mice. The structure shows that ZIKV-117 Fabs cross-link the monomers within the surface E glycoprotein dimers as well as between neighbouring dimers, thus preventing the reorganization of E protein monomers into fusogenic trimers in the acidic environment of endosomes.
Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration
Authors: Sun Xiaohuan , Cahill James , Van Hautegem Tom , Feys Kim , Whipple Clinton , Novák Ondrej , Delbare Sofie , Versteele Charlot , Demuynck Kirin , De Block Jolien , Storme Veronique , Claeys Hannes , Van Lijsebettens Mieke , Coussens Griet , Ljung Karin , De Vliegher Alex , Muszynski Michael , Inzé Dirk , Nelissen Hilde 
Published: March 2017

AbstractMaize is the highest yielding cereal crop grown worldwide for grain or silage. Here, we show that modulating the expression of the maizePLASTOCHRON1(ZmPLA1) gene, encoding a cytochrome P450 (CYP78A1), results in increased organ growth, seedling vigour, stover biomass and seed yield. The engineered trait is robust as it improves yield in an inbred as well as in a panel of hybrids, at several locations and over multiple seasons in the field. Transcriptome studies, hormone measurements and the expression of the auxin responsive DR5rev:mRFPer marker suggest that PLA1 may function through an increase in auxin. Detailed analysis of growth over time demonstrates that PLA1 stimulates the duration of leaf elongation by maintaining dividing cells in a proliferative, undifferentiated state for a longer period of time. The prolonged duration of growth also compensates for growth rate reduction caused by abiotic stresses.
Superfast assembly and synthesis of gold nanostructures using nanosecond low-temperature compression via magnetic pulsed power
Authors: Li Binsong , Bian Kaifu , Lane J. Matthew D. , Salerno K. Michael , Grest Gary S. , Ao Tommy , Hickman Randy , Wise Jack , Wang Zhongwu , Fan Hongyou 
Published: March 2017

AbstractGold nanostructured materials exhibit important size- and shape-dependent properties that enable a wide variety of applications in photocatalysis, nanoelectronics and phototherapy. Here we show the use of superfast dynamic compression to synthesize extended gold nanostructures, such as nanorods, nanowires and nanosheets, with nanosecond coalescence times. Using a pulsed power generator, we ramp compress spherical gold nanoparticle arrays to pressures of tens of GPa, demonstrating pressure-driven assembly beyond the quasi-static regime of the diamond anvil cell. Our dynamic magnetic ramp compression approach produces smooth, shockless (that is, isentropic) one-dimensional loading with low-temperature states suitable for nanostructure synthesis. Transmission electron microscopy clearly establishes that various gold architectures are formed through compressive mesoscale coalescences of spherical gold nanoparticles, which is further confirmed by in-situ synchrotron X-ray studies and large-scale simulation. This nanofabrication approach applies magnetically driven uniaxial ramp compression to mimic established embossing and imprinting processes, but at ultra-short (nanosecond) timescales.
The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries
Authors: Grasso Silvia , Chapelle Jennifer , Salemme Vincenzo , Aramu Simona , Russo Isabella , Vitale Nicoletta , Verdun di Cantogno Ludovica , Dallaglio Katiuscia , Castellano Isabella , Amici Augusto , Centonze Giorgia , Sharma Nanaocha , Lunardi Serena , Cabodi Sara , Cavallo Federica , Lamolinara Alessia , Stramucci Lorenzo , Moiso Enrico , Provero Paolo , Albini Adriana , Sapino Anna , Staaf Johan , Di Fiore Pier Paolo , Bertalot Giovanni , Pece Salvatore , Tosoni Daniela , Confalonieri Stefano , Iezzi Manuela , Di Stefano Paola , Turco Emilia , Defilippi Paola 
Published: March 2017

AbstractThe docking protein p140Cap negatively regulates tumour cell features. Its relevance on breast cancer patient survival, as well as its ability to counteract relevant cancer signalling pathways, are not fully understood. Here we report that in patients with ERBB2-amplified breast cancer, a p140Cap-positive status associates with a significantly lower probability of developing a distant event, and a clear difference in survival. p140Cap dampens ERBB2-positive tumour cell progression, impairing tumour onset and growth in the NeuT mouse model, and counteracting epithelial mesenchymal transition, resulting in decreased metastasis formation. One major mechanism is the ability of p140Cap to interfere with ERBB2-dependent activation of Rac GTPase-controlled circuitries. Our findings point to a specific role of p140Cap in curbing the aggressiveness of ERBB2-amplified breast cancers and suggest that, due to its ability to impinge on specific molecular pathways, p140Cap may represent a predictive biomarker of response to targeted anti-ERBB2 therapies.