bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of an HPLC Method for the Simultaneous Determination of Fipronil, Chlorfenapyr, and Pyriproxyfen in Insecticide Formulations
Authors: Hafeez Anum , Tawab Iffat Abdul , Iqbal Sajid 
Published: July 2016

Abstract
In this study, the analytical method development and validation of an HPLC assay for simultaneous determination of fipronil, chlorfenapyr, and pyriproxyfen in formulation products is described. On the basis of solubility and chromatographic separation with good resolution, acetonitrile–water (80 + 20) was selected as the mobile phase in isocratic mode with a flow rate of 1 mL/min. Chromatographic separations were performed on a Beckman C18 analytical column (4.6 mm × 15 cm, 5 μm particle size; Musa Jee & Sons, Karachi, Pakistan). The retention times for fipronil, chlorfenapyr, and pyriproxyfen were 3.70, 8.61 and 10.09 min, respectively. Calibration curves of all studied insecticides were linear in the concentration range of 20 to 800 μg/mL, with R2 &gt; 0.997. The LODs of fipronil, chlorfenapyr, and pyriproxyfen were 15.1, 13.3, and 20.0 μg/mL, respectively, whereas the LOQs were 45.9, 40.3, and 60.6 μg/mL. Interday precision was RSD, % &lt;2 for all formulation types, whereas intraday precision was &lt;3. The accuracy of the proposed method was determined by interlaboratory comparison. The z-score for all formulation results were &lt;2.The proposed method is low-cost, green, accurate, and precise and can suitably be used for the simultaneous quantitative determination of fipronil, chlorfenapyr, and pyriproxyfen in their formulations.
Human Endomyocardial Biopsy Specimen-Derived Stromal Cells Modulate Angiotensin II-Induced Cardiac Remodeling
Authors: Miteva Kapka , Van Linthout Sophie , Pappritz Kathleen , Müller Irene , Spillmann Frank , Haag Marion , Stachelscheid Harald , Ringe Jochen , Sittinger Michael , Tschöpe Carsten 
Published: July 2016

AbstractCardiac-derived adherent proliferating cells (CardAPs) are cells derived from human endomyocardial biopsy specimens; they share several properties with mesenchymal stromal cells. The aims of this study were to evaluate whether intramyocardial injection of CardAPs modulates cardiac fibrosis and hypertrophy in a mouse model of angiotensin II (Ang II)-induced systolic heart failure and to analyze underlying mechanisms. Intramyocardial application of 200,000 CardAPs improved left ventricular function. This was paralleled by a decline in left ventricular remodeling, as indicated by a reduction in cardiac fibrosis and hypertrophy. CardAPs reduced the ratio of the left ventricle to body weight and cardiac myosin expression (heavy chain), and decreased the Ang II-induced phosphorylation state of the cardiomyocyte hypertrophy mediators Akt, extracellular-signal regulated kinase (ERK) 1, and ERK2. In accordance with the antifibrotic and antihypertrophic effects of CardAPs shown in vivo, CardAP supplementation with cardiac fibroblasts decreased the Ang II-induced reactive oxygen species production, α-SMA expression, fibroblast proliferation, and collagen production. Coculture of CardAPs with HL-1 cardiomyocytes downregulated the Ang II-induced expression of myosin in HL-1. All antifibrotic and antihypertrophic features of CardAPs were mediated in a nitric oxide- and interleukin (IL)-10-dependent manner. Moreover, CardAPs induced a systemic immunomodulation, as indicated by a decrease in the activity of splenic mononuclear cells and an increase in splenic CD4CD25FoxP3, CD4-IL-10, and CD8-IL-10 T-regulatory cells in Ang II mice. Concomitantly, splenocytes from Ang II CardAPs mice induced less collagen in fibroblasts compared with splenocytes from Ang II mice. We conclude that CardAPs improve Ang II-induced cardiac remodeling involving antifibrotic and antihypertrophic effects via paracrine actions and immunomodulatory properties.SignificanceDespite effective pharmacological treatment with angiotensin II type I receptor antagonists or angiotensin II-converting enzyme inhibitors, morbidity and mortality associated with heart failure are still substantial, prompting the search of novel therapeutic strategies. There is accumulating evidence supporting the use of cell therapy for cardiac repair. This study demonstrates that cells derived from human endomyocardial biopsies, cardiac-derived adherent proliferating cells (CardAPs), have the potential to reduce angiotensin II-induced cardiac remodeling and improve left ventricular function in angiotensin II mice. The mechanism involves antifibrotic and antihypertrophic effects via paracrine actions and immunomodulatory properties. These findings support the potential of CardAPs for the treatment of heart failure.
Smooth Muscle Progenitor Cells Derived From Human Pluripotent Stem Cells Induce Histologic Changes in Injured Urethral Sphincter
Authors: Li Yanhui , Wen Yan , Wang Zhe , Wei Yi , Wani Prachi , Green Morgaine , Swaminathan Ganesh , Ramamurthi Anand , Pera Renee Reijo , Chen Bertha 
Published: July 2016

Abstract
Data suggest that myoblasts from various sources, including bone marrow, skeletal muscle, and adipose tissue, can restore muscle function in patients with urinary incontinence. Animal data have indicated that these progenitor cells exert mostly a paracrine effect on the native tissues rather than cell regeneration. Limited knowledge is available on the in vivo effect of human stem cells or muscle progenitors on injured muscles. We examined in vivo integration of smooth muscle progenitor cells (pSMCs) derived from human pluripotent stem cells (hPSCs). pSMCs were derived from a human embryonic stem cell line (H9-ESCs) and two induced pluripotent stem cell (iPSC) lines. pSMCs were injected periurethrally into urethral injury rat models (2 × 106 cells per rat) or intramuscularly into severe combined immunodeficiency mice. Histologic and quantitative image analysis revealed that the urethras in pSMC-treated rats contained abundant elastic fibers and thicker muscle layers compared with the control rats. Western blot confirmed increased elastin/collagen III content in the urethra and bladder of the H9-pSMC-treated rats compared with controls. iPSC-pSMC treatment also showed similar trends in elastin and collagen III. Human elastin gene expression was not detectable in rodent tissues, suggesting that the extracellular matrix synthesis resulted from the native rodent tissues rather than from the implanted human cells. Immunofluorescence staining and in vivo bioluminescence imaging confirmed long-term engraftment of pSMCs into the host urethra and the persistence of the smooth muscle phenotype. Taken together, the data suggest that hPSC-derived pSMCs facilitate restoration of urethral sphincter function by direct smooth muscle cell regeneration and by inducing native tissue elastin/collagen III remodeling.

Significance
The present study provides evidence that a pure population of human smooth muscle progenitor cells (pSMCs) derived from human pluripotent stem cells (hPSCs) (human embryonic stem cells and patient induced pluripotent stem cells) restores urethral sphincter function by two mechanisms: modulation of extracellular matrix protein metabolism in vivo and pSMC proliferation and differentiation into smooth muscle cells to regenerate the muscle layer in the lower urinary tract. These findings on the in vivo effects of human pSMCs should aid in optimizing regenerative therapies using human myoblasts.
Health on a Cloud: Modeling Digital Flows in an E-health Ecosystem
Authors: Stephanie Felix Lena , Sharma Ravi S. 
Published: July 2016

A unified and well-knit e-health network is one that provides a common platform to its key stakeholders to facilitate a sharing of information with a view to promoting cooperation and maximizing benefits. A promising candidate worthy of being considered for this ponderous job is the emerging ‘cloud technology’ with its offer of computing as a utility, which seems well-suited to foster such a network bringing together diverse players who would otherwise remain fragmented and be unable to reap benefits that accrue from cooperation. The e-health network serves to provide added value to its various stakeholders through syndication, aggregation and distribution of this health information, thereby reducing costs and improving efficiencies. Because such a network is in fact an interconnected ‘network of networks’ that delivers a product or service through both competition and cooperation, it can be thought of as a business ecosystem. . This study attempts to model the digital information flows in an e-health ecosystem and analyze the resulting strategic implications for the key players for whom the rules of the game are bound to change given their interdependent added-values. The ADVISOR framework is deployed to examine the values created and captured in the ecosystem. Based on this analysis, some critical questions that must be addressed as necessary preconditions for an e-Health Cloud, are derived. The paper concludes with the conjecture that “collaboration for value” will replace “competition for revenue” as the new axiom in the health care business that could ideally usher in a fair, efficient and sustainable ecosystem.
Investigation of the giant step bunching induced by the etching of 4H-SiC in Ar–H2 mix gases
Authors: Ishida Yuuki , Yoshida Sadafumi 
Published: July 2016

Abstract
We have investigated the etching of 4H-SiC by H2 and the occurrence of giant step bunching (GSB) on SiC surfaces in an Ar–H2 gas system. In this paper, we propose a new method of determining the rate-limiting process under a certain etching condition and a new chemical kinetics model of SiC etching at the mass transfer limit in the Ar–H2 gas system. Using the new determination method, we have found that GSB occurs only at the mass transfer limit. Combining the new chemical kinetics model with the cluster effect model of the GSB occurrence mechanism, we have derived the theoretical boundary line for the temperature dependence of H2 concentration on GSB occurrence and shown the good agreement between the theoretical boundary line and the line derived from experimental results.
Plasmonic lens for ultraviolet wavelength
Authors: Takeda Minoru , Tanimoto Takuya , Inoue Tsutomu , Aizawa Kento 
Published: July 2016

Abstract
A plasmonic lens (PL) is one of the promising photonic devices utilizing the surface plasmon wave. In this study, we have newly developed a PL with a 3.5 µm diameter for a wavelength of 375 nm (ultraviolet region). It is composed of multiple circular slit apertures milled in aluminum (Al) thin film. We have simulated the electric field distribution of the PL, and confirmed that a tightly focused beam spot of subwavelength size in the far-field region was attained. We have also measured the focusing characteristics of the PL using a near-field scanning optical microscope (NSOM) and compared them with the calculated results.
Hochauflösende NMR‐spektroskopische Bestimmung der dynamischen Struktur von Membranproteinen
Authors: Jaremko Mariusz , Jaremko Łukasz , Villinger Saskia , Schmidt Christian D. , Griesinger Christian , Becker Stefan , Zweckstetter Markus 
Published: July 2016

Abstract15N‐Spinrelaxationsraten können wichtige Informationen über Fernstruktur und interne Bewegungen von Membranproteinen bereitstellen. Kombiniert mit einem verbesserten Berechnungsprotokoll offenbart die mittels Relaxationsraten abgeleitete Struktur des 283 Reste umfassenden humanen spannungsabhängigen Anionenkanals ein anisotrop geformtes Fass mit einer fest verankerten N‐terminalen Helix. Diese Arbeit eröffnet somit einen NMR‐spektroskopischen Ansatz, um die Struktur und Dynamik von Membranproteinen der Säugetiere mit hoher Exaktheit und Auflösung zu bestimmen.
A Cyclized Helix‐Loop‐Helix Peptide as a Molecular Scaffold for the Design of Inhibitors of Intracellular Protein–Protein Interactions by Epitope and Arginine Grafting
Authors: Fujiwara Daisuke , Kitada Hidekazu , Oguri Masahiro , Nishihara Toshio , Michigami Masataka , Shiraishi Kazunori , Yuba Eiji , Nakase Ikuhiko , Im Haeri , Cho Sunhee , Joung Jong Young , Kodama Seiji , Kono Kenji , Ham Sihyun , Fujii Ikuo 
Published: July 2016

AbstractThe design of inhibitors of intracellular protein–protein interactions (PPIs) remains a challenge in chemical biology and drug discovery. We propose a cyclized helix‐loop‐helix (cHLH) peptide as a scaffold for generating cell‐permeable PPI inhibitors through bifunctional grafting: epitope grafting to provide binding activity, and arginine grafting to endow cell‐permeability. To inhibit p53–HDM2 interactions, the p53 epitope was grafted onto the C‐terminal helix and six Arg residues were grafted onto another helix. The designed peptide cHLHp53‐R showed high inhibitory activity for this interaction, and computational analysis suggested a binding mode for HDM2. Confocal microscopy of cells treated with fluorescently labeled cHLHp53‐R revealed cell membrane penetration and cytosolic localization. The peptide inhibited the growth of HCT116 and LnCap cancer cells. This strategy of bifunctional grafting onto a well‐structured peptide scaffold could facilitate the generation of inhibitors for intracellular PPIs.
Ansätze zur Synthese Trifluormethoxy‐substituierter Verbindungen
Authors: Tlili Anis , Toulgoat Fabien , Billard Thierry 
Published: July 2016

AbstractAufgrund der einzigartigen Eigenschaften der Trifluormethoxygruppe finden Moleküle, die diesen Baustein enthalten, in verschiedenen Bereichen Anwendung, besonders in den Biowissenschaften. Obwohl also an dieser funktionellen Gruppe ein großes Interesse besteht, ist gegenwärtig nur eine geringe Anzahl an trifluormethoxylierten Molekülen präparativ zugänglich. In den letzten Jahren sind mehrere innovative und vielversprechende Strategien für die Synthese von trifluormethoxylierten Verbindungen beschrieben worden. In diesem Kurzaufsatz werden diese Methoden diskutiert, mit einem Fokus auf aktuelleren Fortschritten.
Engineering of CH3NH3PbI3 Perovskite Crystals by Alloying Large Organic Cations for Enhanced Thermal Stability and Transport Properties
Authors: Peng Wei , Miao Xiaohe , Adinolfi Valerio , Alarousu Erkki , El Tall Omar , Emwas Abdul‐Hamid , Zhao Chao , Walters Grant , Liu Jiakai , Ouellette Olivier , Pan Jun , Murali Banavoth , Sargent Edward H. , Mohammed Omar F. , Bakr Osman M. 
Published: July 2016

AbstractThe number of studies on organic–inorganic hybrid perovskites has soared in recent years. However, the majority of hybrid perovskites under investigation are based on a limited number of organic cations of suitable sizes, such as methylammonium and formamidinium. These small cations easily fit into the perovskite's three‐dimensional (3D) lead halide framework to produce semiconductors with excellent charge transport properties. Until now, larger cations, such as ethylammonium, have been found to form 2D crystals with lead halide. Here we show for the first time that ethylammonium can in fact be incorporated coordinately with methylammonium in the lattice of a 3D perovskite thanks to a balance of opposite lattice distortion strains. This inclusion results in higher crystal symmetry, improved material stability, and markedly enhanced charge carrier lifetime. This crystal engineering strategy of balancing opposite lattice distortion effects vastly increases the number of potential choices of organic cations for 3D perovskites, opening up new degrees of freedom to tailor their optoelectronic and environmental properties.
Zelluläre Mikroskopie der Poly(ADP‐Ribos)ylierung von Proteinen in Echtzeit
Authors: Buntz Annette , Wallrodt Sarah , Gwosch Eva , Schmalz Michael , Beneke Sascha , Ferrando‐May Elisa , Marx Andreas , Zumbusch Andreas 
Published: July 2016

AbstractPoly(ADP‐Ribos)ylierung (PARylierung) ist eine wichtige posttranslationale Proteinmodifikation, die in grundlegende zelluläre Prozesse wie Genregulation und DNA‐Reparatur involviert ist. Ihre Fehlregulierung wurde mit verschiedenen Krankheiten wie Krebs in Verbindung gebracht. Trotz größter Wichtigkeit gibt es nur wenige Methoden, um PARylierung und ihre Dynamik in Zellen zu beobachten. Mittels einer chemisch‐biologischen Herangehensweise entwickelten wir ein fluoreszierendes NAD+‐Analogon, das sich als kompetitiver Baustein für die PARylierung von Proteinen in vitro und in Zellen erwies. Dies ermöglichte uns, den Umsatz von PAR direkt und in lebenden Zellen nach DNA‐Schädigung durch NIR‐Mikrobestrahlung zu verfolgen. Zusätzlich wurden mithilfe von FLIM‐FRET‐Mikroskopie kovalente und nichtkovalente Interaktionen von PAR mit ausgewählten Proteinen sichtbar gemacht. Unsere Ergebnisse eröffnen neue Chancen für die schnelle zelluläre Untersuchung der Protein‐PARylierung in Echtzeit und werden somit zu einem besseren Verständnis und höherer Aussagekraft im zellulären Kontext beitragen.
Graphitic Carbon Nitride/Nitrogen‐Rich Carbon Nanofibers: Highly Efficient Photocatalytic Hydrogen Evolution without Cocatalysts
Authors: Han Qing , Wang Bing , Gao Jian , Qu Liangti 
Published: July 2016

AbstractAn interconnected framework of mesoporous graphitic‐C3N4 nanofibers merged with in situ incorporated nitrogen‐rich carbon has been prepared. The unique composition and structure of the nanofibers as well as strong coupling between the components endow them with efficient light‐harvesting properties, improved charged separation, and a multidimensional electron transport path that enhance the performance of hydrogen production. The as‐obtained catalyst exhibits an extremely high hydrogen‐evolution rate of 16885 μmol h−1 g−1, and a remarkable apparent quantum efficiency of 14.3 % at 420 nm without any cocatalysts, which is much higher than most reported g‐C3N4‐based photocatalysts even in the presence of Pt‐based cocatalysts.
Isolation and Characterization, Including by X‐ray Crystallography, of Contact and Solvent‐Separated Ion Pairs of Silenyl Lithium Species
Authors: Pinchuk Daniel , Mathew Jomon , Kaushansky Alexander , Bravo‐Zhivotovskii Dmitry , Apeloig Yitzhak 
Published: July 2016

AbstractReaction of bromoacylsilane 1 (pink solution) with tBu2MeSiLi (3.5 equiv) in a 4:1 hexane:THF solvent mixture at −78 °C to room temperature yields the solvent separated ion pair (SSIP) of silenyl lithium E‐[(tBuMe2Si)(tBu2MeSi)C=Si(SiMetBu2)]− [Li⋅4THF]+ 2 a (green–blue solution). Removal of the solvent and addition of benzene converts 2 a into the corresponding contact ion pair (CIP) 2 b (violet–red solution) with two THF molecules bonded to the lithium atom. The 2 a⇌2 b interconversion is reversible upon THF⇌ benzene solvent change. Both 2 a and 2 b were characterized by X‐ray crystallography, NMR and UV/Vis spectroscopy, and theoretical calculations. The degree of dissociation of the Si−Li bond has a large effect on the visible spectrum (and thus color) and on the silenylic 29Si NMR chemical shift, but a small effect on the molecular structure. This is the first report of the X‐ray molecular structure of both the SSIP and the CIP of any R2E=E′RM species (E=C, Si; E′=C, Si; M=metal).
From Cluster to Polymer: Ligand Cone Angle Controlled Syntheses and Structures of Copper(I) Alkynyl Complexes
Authors: Chang Xiao‐Yong , Low Kam‐Hung , Wang Juan‐Yu , Huang Jie‐Sheng , Che Chi‐Ming 
Published: July 2016

AbstractCopper(I) alkynyl complexes have attracted tremendous attention in structural studies, as luminescent materials, and in catalysis, and homoleptic complexes have been reported to form polymers or large clusters. Herein, six unprecedented structures of CuI alkynyl complexes and a procedure to measure the cone angles of alkynyl ligands based on the crystal structures of these complexes are reported. An increase of the alkynyl cone angle in the complexes leads to a modulation of the structures from polymeric [((PhC≡CC≡C)Cu)2(NH3)]∞, to a large cluster [(TripC≡CC≡C)Cu]20(MeCN)4, to a relatively small cluster [(TripC≡C)Cu]8 (Trip=2,4,6‐iPr3‐C6H2). The complexes exhibit yellow‐to‐red phosphorescence at ambient temperature in the solid state and the luminescence behavior of the Cu20 cluster is sensitive to acetonitrile.
A Bispidine Iron(IV)–Oxo Complex in the Entatic State
Authors: Comba Peter , Fukuzumi Shunichi , Koke Carsten , Martin Bodo , Löhr Anna‐Maria , Straub Johannes 
Published: July 2016

AbstractFor a series of FeIV=O complexes with tetra‐ and pentadentate bispidine ligands, the correlation of their redox potentials with reactivity, involving a variety of substrates for alkane hydroxylation (HAT), alkene epoxidation, and phosphine and thioether oxidation (OAT) are reported. The redox potentials span approximately 350 mV and the reaction rates over 8 orders of magnitude. From the experimental data and in comparison with published studies it emerges that electron transfer and the driving force are of major importance, and this is also supported by the DFT‐based computational analysis. The striking difference of reactivity of two isomeric systems with pentadentate bispidines is found to be due to a destabilization of the S=1 ground state of one of the ferryl isomers, and this is supported by the experimentally determined redox potentials and published stability constants with a series of first‐row transition metal ions with these two isomeric ligands.
Synthesis of poly(N‐isopropylacrylamide) by distillation precipitation polymerization and quantitative grafting on mesoporous silica
Authors: Jadhav Sushilkumar A. , Brunella Valentina , Miletto Ivana , Berlier Gloria , Scalarone Dominique 
Published: July 2016

ABSTRACTIn this work, syntheses of thermoresponsive poly(N‐isopropylacrylamide) (PNIPAM) with different molecular weights were carried out in ethanol by distillation precipitation polymerization (DPP) technique. The synthesized polymers were fully characterized by attenuated total reflection Fourier‐transform infrared (ATR‐FTIR) spectroscopy, nuclear magnetic resonance spectroscopy, and size exclusion chromatography techniques. The lower critical solution temperatures of the polymers were determined with differential scanning calorimetry. A simple and versatile method for the in situ synthesis and grafting of PNIPAM on mesoporus silica nanoparticles (MSNs) with improved control over quantitative grafting is devised. The PNIPAM grafted MSNs were characterized with ATR‐FTIR, thermogravimetric analysis, transmission electron microscopy, and dynamic light scattering analyses. From the results obtained it is showed that quantitative grafting of PNIPAM on MSNs from 1 to 20% by weight can be tuned by manipulating the in situ DPP reaction conditions. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44181.
Causes of excessive late death after trauma compared with a matched control cohort
Authors: Eriksson M , Brattström O , Larsson E , Oldner A 
Published: July 2016

Abstract

Background
Studies on mortality following trauma have been restricted mainly to in-hospital or 30-day death. Mortality risk may be sustained several years after trauma, but the causes of late death have not been elucidated. The aim was to investigate mortality and analyse causes of late death after trauma.

Methods
All injured patients from a regional trauma registry with long-term follow-up were matched in a 1 : 5 ratio with uninjured controls by age, sex and municipality. By linkage to national registries, long-term mortality, causes of death and co-morbidity status were identified. Excess mortality was examined by calculating the all-cause mortality rate ratio (MRR).

Results
Among the trauma cohort of 7382 patients, 662 (9·0 per cent) died within 3 years after the index trauma; the 30-day mortality rate was 5·0 per cent. Compared with the control group (36 759 individuals), there was a sustained increase in mortality up to 3 years after trauma; the MRR was 2·88 (95 per cent c.i. 2·37 to 3·50) for days 31–365, 1·59 (1·24 to 2·04) for years 1–2 and 1·43 (1·06 to 1·92) for years 2–3. External causes, including new trauma, were far more common causes of late death in injured patients than in matched controls.

Conclusion
Postinjury mortality is increased for several years after trauma. Excess mortality is largely attributed to recurrent trauma and other external causes of death.