bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined treatment with Aronia berry extract and oligomeric proanthocyanidins exhibit a synergistic anticancer efficacy through LMNB1‐AKT signaling pathways in colorectal cancer
Authors: Li Yuan , Xu Caiming , Weng Wenhao , Goel Ajay 
Published: September 2024

AbstractColorectal cancer (CRC) is one of the most prevalent and highly recurrent malignancies worldwide and currently ranks as the second leading cause of cancer‐related deaths. The high degree of morbidity and mortality associated with CRC is primarily attributed to the limited effectiveness of current therapeutic approaches and the emergence of chemoresistance to standard treatment modalities. Recent research indicates that several natural products, including Aronia berry extracts (ABE) and oligomeric proanthocyanidins (OPCs), might offer a safe, cost‐effective, and multitargeted adjunctive role to cancer treatment. Herein, we hypothesized a combined treatment with ABE and OPCs could synergistically modulate multiple oncogenic pathways in CRC, thereby enhancing their anticancer activity. We initially conducted a series of in vitro experiments to assess the synergistic anticancer effects of ABE and OPCs on CRC cell lines. We demonstrate that these two compounds exhibited a superior synergistic anticancer potential versus individual treatments in enhancing the ability to inhibit cell viability, suppress colony formation, and induce apoptosis (p < 0.05). Consistent with our in vitro findings, we validated this combinatorial anticancer effect in tumor‐derived 3D organoids (PDOs; p < 0.01). Using genome‐wide transcriptomic profiling, we identified that a specific gene, LMNB1, associated with the cell apoptosis pathway, was found to play a crucial role in exhibiting anticancer effects with these two products. Furthermore, the combined treatment of ABE and OPCs significantly impacted the expression of key proteins involved in apoptosis, including suppressed expression levels of LMNB1 in CRC cell lines (p < 0.05), which resulted in inhibiting downstream AKT phosphorylation. In conclusion, our study provides novel evidence of the synergistic anticancer effects of ABE and OPCs in CRC cells, partially mediated through the regulation of apoptosis and the oncogene LMNB1 within the AKT signaling pathway. These findings have the potential to better appreciate the anticancer potential of natural products in CRC and help improve treatment outcomes in this malignancy.
NeuroBooster Array: A Genome‐Wide Genotyping Platform to Study Neurological Disorders Across Diverse Populations
Authors: Bandres‐Ciga Sara , Faghri Faraz , Majounie Elisa , Koretsky Mathew J. , Kim Jeffrey , Levine Kristin S. , Leonard Hampton , Makarious Mary B. , Iwaki Hirotaka , Crea Peter Wild , Hernandez Dena G. , Arepalli Sampath , Billingsley Kimberley , Lohmann Katja , Klein Christine , Lubbe Steven J. , Jabbari Edwin , Saffie‐Awad Paula , Narendra Derek , Reyes‐Palomares Armando , Quinn John P. , Schulte Claudia , Morris Huw R. , Traynor Bryan J. , Scholz Sonja W. , Houlden Henry , Hardy John , Dumanis Sonya , Riley Ekemini , Blauwendraat Cornelis , Singleton Andrew , Nalls Mike , Jeff Janina , Vitale Dan ,   the Global Parkinson's Genetics Program (GP2) and the Center for Alzheimer's and Related Dementias (CARD)
Published: September 2024

AbstractBackgroundCommercial genome‐wide genotyping arrays have historically neglected coverage of genetic variation across populations.ObjectiveWe aimed to create a multi‐ancestry genome‐wide array that would include a wide range of neuro‐specific genetic content to facilitate genetic research in neurological disorders across multiple ancestral groups, fostering diversity and inclusivity in research studies.MethodsWe developed the Illumina NeuroBooster Array (NBA), a custom high‐throughput and cost‐effective platform on a backbone of 1,914,934 variants from the Infinium Global Diversity Array and added custom content comprising 95,273 variants associated with more than 70 neurological conditions or traits, and we further tested its performance on more than 2000 patient samples. This novel platform includes approximately 10,000 tagging variants to facilitate imputation and analyses of neurodegenerative disease–related genome‐wide association study loci across diverse populations.ResultsIn this article, we describe NBA's potential as an efficient means for researchers to assess known and novel disease genetic associations in a multi‐ancestry framework. The NBA can identify rare genetic variants and accurately impute more than 15 million common variants across populations. Apart from enabling sample prioritization for further whole‐genome sequencing studies, we envisage that NBA will play a pivotal role in recruitment for interventional studies in the precision medicine space.ConclusionsFrom a broader perspective, the NBA serves as a promising means to foster collaborative research endeavors in the field of neurological disorders worldwide. Ultimately, this carefully designed tool is poised to make a substantial contribution to uncovering the genetic etiology underlying these debilitating conditions. © 2024 The Author(s). Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Artificial intelligence in orthopaedic education: A comparative analysis of ChatGPT and Bing AI's Orthopaedic In‐Training Examination performance
Authors: Chen Clark J. , Bilolikar Vivek K. , VanNest Duncan , Raphael James , Shaffer Gene 
Published: September 2024

AbstractBackgroundThis study evaluated the performance of generative artificial intelligence (AI) models on the Orthopaedic In‐Training Examination (OITE), an annual exam administered to U.S. orthopaedic residency programs.MethodsChatGPT 3.5 and Bing AI GPT 4.0 were evaluated on standardised sets of multiple‐choice questions drawn from the American Academy of Orthopaedic Surgeons OITE online question bank spanning 5 years (2018–2022). A total of 1165 questions were posed to each AI system. The performance of both systems was standardised using the latest versions of ChatGPT 3.5 and Bing AI GPT 4.0. Historical data of resident scores taken from the annual OITE technical reports was used as a comparison.ResultsAcross the five datasets, ChatGPT 3.5 scored an average of 55.0% on the OITE questions. Bing AI GPT 4.0 scored higher with an average of 80.0%. In comparison, the average performance of orthopaedic residents in national accredited programs was 62.1%. Bing AI GPT 4.0 outperformed ChatGPT 3.5 and Accreditation Council for Graduate Medical Education examinees, and analysis of variance analysis demonstrated p < 0.001 among groups. The best performance was by Bing AI GPT 4.0 on OITE 2020.ConclusionGenerative AI can provide a logical context across answer responses through its in‐depth information searches and citation of resources. This combination presents a convincing argument for the possible uses of AI in medical education as an interactive learning aid.
Teaching foundational skills using strategic incremental rehearsal: Investigating effectiveness across varying school agents
Authors: Novelli Christina , Ardoin Scott P. 
Published: September 2024

AbstractStrategic incremental rehearsal (SIR) is an efficacious flashcard intervention shown to improve foundational skill acquisition. However, there is limited research examining the feasibility and effectiveness of SIR when used by school‐based implementers. We present two studies to address this limitation. Study 1 examined the effects of SIR implemented by teachers on the acquisition of grapheme‐phoneme correspondences with three at‐risk kindergarteners. Study 2 examined the effects of SIR implemented by a same‐age peer on the acquisition and fluency of addition math facts with 3 second‐grade students receiving special education services. Overall, both studies point to the effectiveness of using SIR with students requiring intensive intervention in an focused, short timeframe. The effectiveness of SIR is particularly salient given that teachers and peers were able to implement SIR with high treatment integrity and effectively improve outcomes for students with and at risk for disabilities.
Matrix stiffness regulates the protein profile of extracellular vesicles of pancreatic cancer cell lines
Authors: Ferrara Benedetta , Bourgoin‐Voillard Sandrine , Habert Damien , Vallée Benoit , Nicolas‐Boluda Alba , Simanic Isidora , Seve Michel , Vingert Benoit , Gazeau Florence , Castellano Flavia , Cohen José , Courty José , Cascone Ilaria 
Published: September 2024

AbstractThe fibrotic stroma characterizing pancreatic ductal adenocarcinoma (PDAC) derives from a progressive tissue rigidification, which induces epithelial mesenchymal transition and metastatic dissemination. The aim of this study was to investigate the influence of matrix stiffness on PDAC progression by analyzing the proteome of PDAC‐derived extracellular vesicles (EVs). PDAC cell lines (mPDAC and KPC) were grown on synthetic supports with a stiffness close to non‐tumor (NT) or tumor tissue (T), and the protein expression levels in cell‐derived EVs were analyzed by a quantitative MSE label‐free mass spectrometry approach. Our analysis figured out 15 differentially expressed proteins (DEPs) in mPDAC‐EVs and 20 DEPs in KPC‐EVs in response to matrix rigidification. Up‐regulated proteins participate to the processes of metabolism, matrix remodeling, and immune response, altogether hallmarks of PDAC progression. A multimodal network analysis revealed that the majority of DEPs are strongly related to pancreatic cancer. Interestingly, among DEPs, 11 related genes (ACTB/ANXA7/C3/IGSF8/LAMC1/LGALS3/PCD6IP/SFN/TPM3/VARS/YWHAZ) for mPDAC‐EVs and 9 (ACTB/ALDH2/GAPDH/HNRNPA2B/ITGA2/NEXN/PKM/RPN1/S100A6) for KPC‐EVs were significantly overexpressed in tumor tissues according to gene expression profiling interaction analysis (GEPIA). Concerning the potential clinical relevance of these data, the cluster of ACTB, ITGA2, GAPDH and PKM genes displayed an adverse effect (p < 0.05) on the overall survival of PDAC patients.
Assessment of lower respiratory tract microbiota associated with pulmonary tuberculosis in children
Authors: Yang Xuemei , Jiao Weiwei , Zeng Xi , Yu Jie , Xiao Jing , Jiang Tingting , Tang He , Bi Jing , Chen Yiyi , Li Xiaoxue , Chen Wanning , Chen Yu , Shen Adong , Sun Lin 
Published: September 2024

AbstractBackgroundThe respiratory microbiota plays a crucial role in the development of tuberculosis (TB). While existing research has underscored imbalances in the respiratory microbiota of adult patients with TB, information regarding the lower respiratory tract (LRT) microbiota in pediatric patients with TB remains scarce.MethodsWe employed 16S rRNA gene sequencing technology to investigate the LRT microbial communities of 85 children of different ages with active TB of different severities, 33 children with infectious diseases other than TB, and 48 sex‐ and age‐matched healthy children.ResultsA marked imbalance in the respiratory microbiota was observed in children with TB, highlighted by reduced alpha diversity and a distinct microbial community structure. Comparative analysis indicated that patients with severe TB exhibited lower Neisseria levels than those with non‐severe TB (1.01% vs. 3.93%, respectively; p = .02). Streptococcus and Gemella levels were lower in bacteriologically confirmed TB cases compared with clinically diagnosed cases, and higher in healthy children younger than 10 years old than in the older group. Spearman correlation analysis demonstrated significant associations between the microbiota of the LRT and cytokine concentrations in the sputum of children with TB (e.g., an inverse correlation between Veillonella and interleukin‐17A).ConclusionsTB induced significant dysbiosis in the LRT microbiota of children that was associated with disease severity and the immunological response in the respiratory tract. Our findings may offer a deeper understanding of the role of the respiratory microbiome in TB pathogenesis and progression.
Integrating novel interventional techniques into pediatric pulmonology training
Authors: Schramm Dirk 
Published: September 2024

AbstractObjectiveTo review the evolution of pediatric pulmonology interventions and propose strategies for advancing training in the field.MethodsI examined the historical development of pediatric pulmonology interventions and current training practices, including hands‐on courses and fellowship programs. I reviewed a survey of US pediatric pulmonology centers to assess variability in procedural expertise.ResultsHistorically, foreign body removal dominated pediatric pulmonology interventions. Advancements in technology have expanded the field to include techniques such as endobronchial and transbronchial biopsies, airway lumen restoration, and cryotherapy, enabling more accurate tissue sampling with larger specimens while maintaining safety. Hands‐on courses, offered globally and at major conferences, provide opportunities for skill development, self‐assessment, and networking. However, limited availability leads to high demand and long waiting lists. A survey of US pediatric pulmonology centers revealed significant variability in procedural expertise, highlighting the need for uniform training across institutions.ConclusionsTo better integrate interventional techniques, I propose that pediatric pulmonology training could benefit from a structured, tiered approach: (1) expanding hands‐on workshops and incorporating them into fellowship programs, (2) facilitating collaborations between centers of excellence to allow trainees to rotate through institutions with advanced expertise, and (3) developing an additional year of training for an "Advanced Pediatric Pulmonologist" certification. This approach aims to ensure proficiency in the latest interventional techniques, standardize care, and foster advancements across the field.
GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries
Authors: Hoch Shlomo Yakir , Netzer Ravit , Weinstein Jonathan Yaacov , Krauss Lucas , Hakeny Karen , Fleishman Sarel Jacob 
Published: September 2024

AbstractGolden Gate assembly (GGA) can seamlessly generate full‐length genes from DNA fragments. In principle, GGA could be used to design combinatorial mutation libraries for protein engineering, but creating accurate, complex, and cost‐effective libraries has been challenging. We present GGAssembler, a graph‐theoretical method for economical design of DNA fragments that assemble a combinatorial library that encodes any desired diversity. We used GGAssembler for one‐pot in vitro assembly of camelid antibody libraries comprising >105 variants with DNA costs <0.007$ per variant and dropping significantly with increased library complexity. >93% of the desired variants were present in the assembly product and >99% were represented within the expected order of magnitude as verified by deep sequencing. The GGAssembler workflow is, therefore, an accurate approach for generating complex variant libraries that may drastically reduce costs and accelerate discovery and optimization of antibodies, enzymes and other proteins. The workflow is accessible through a Google Colab notebook at https://github.com/Fleishman-Lab/GGAssembler.
Synthesis, Photophysical, Antimicrobial Activity and In‐silico Study of Homoleptic Dithiocarbamate Complexes [MII{k2S^S‐S2CN(R)CH2C6H4OH}2] (R=2‐picolyl, 3‐picolyl; M=NiII, CuII, ZnII)
Authors: Parmar Heena A. , Makwana Bhaveshkumar B. , Singh Vinay K. 
Published: September 2024

AbstractAn array of mononuclear dithiocarbamate complexes [MII{k2S^S‐S2CN(R)CH2C6H4OH}2] (R=2‐picolyl, M=NiII 1, CuII 2, ZnII 3, R=3‐picolyl, M=NiII 4, CuII 5, ZnII 6); incorporating heterocyclic backbone were synthesised from (2‐hydroxybenzyl)(2‐picolyl)amine (HL1) and (2‐hydroxybenzyl)(3‐picolyl)amine (HL2) precursors. The formulation of these complexes was examined using various spectroscopic and analytical techniques (1H NMR, 13C NMR, FTIR, UV‐visible, Fluorescence, TGA/DTA) and further corroborated by DFT level calculation. Notably, precursors HL1 and HL2 exhibits maximum emission in the visible nm region (ca 502 and 408 nm) upon excitation of ultraviolet radiation at ~280 nm with concomitant Stokes shift ca 220 and 128 nm, respectively. The emission properties of these evidently suppressed after complexation with metal ions. Stability of 1–6 were determined by thermogravimetric analysis. The magnetic moment along with UV‐visible of 1–6 suggests square‐planar environment around NiII and CuII and tetrahedral environment around ZnII in 1–6 which is in accord with their optimized geometries. Compounds were screened for in‐ vitro antimicrobial activity against E. coli, P. aeruginosa, S. aureus, S. pyogenes, C. albicans, A. niger, A. clavatus by broth dilution method. Molecular docking study was performed to rationalize the results.
N,S‐Doped Ordered Mesoporous Carbon: An Effective Catalyst for the Formylation of Anilines and Stabilization of Pd Nanoparticles to Catalyze the Hydrogenation of Nitroarenes
Authors: Eftekhari‐Sis Bagher , Jalalat Maryam , Vahdati‐Khajeh Saleh , Samadi Maryam , Alavinia Maryam , Zirak Maryam 
Published: September 2024

AbstractN,S‐Doped highly ordered mesoporous carbon (N,S‐doped OMC) material was constructed by the nanocasting methodology using egg yolk with a high content of amino acids as a carbon precursor and SBA‐15 as a hard template, followed by carbonization at 500 °C under an N2 atmosphere, and then aqueous alkaline‐induced removal of the silica moiety of the SBA‐15 template. Prepared N,S‐doped OMC material efficiently catalyzed the formylation of various substituted anilines using DMF as a formylating agent in water, to afford N‐formylaniline derivatives in high to excellent yields. Also, as‐prepared N,S‐doped OMC was used to immobilize and stabilize the Pd nanoparticles in order to synthesize Pd@OMC material, which was used as an efficient catalyst for the hydrogenation of nitroaromatic compounds to the corresponding aniline derivatives. Nitroaromatic compounds are considered to be highly toxic water contaminant compounds, and their removal from wastewater by the hydrogenation is an interesting method. Furthermore, Pd@OMC catalyzed one‐pot tandem hydrogenation of nitrobenzene/formylation reactions was investigated, which gave corresponding N‐formyl aniline in high yield. Synthesized OMC and Pd@OMC were characterized using X‐ray diffraction (XRD), Brunauer‐Emmett‐Teller (BET), scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy (EDS), and transmission electron microscopy (TEM) analysis.
Induced Ferroptosis Pathway by Regulating Cellular Lipid Peroxidation With Peroxynitrite Generator for Reversing “Cold” Tumors
Authors: Li Ruipeng , Yuan Haitao , Zhang Chuangxin , Han Dong , Wang Yunxia , Feng Liheng 
Published: September 2024

AbstractOvercoming the resistance of tumor cells to apoptosis and immunosuppression is an important challenge to improve tumor immunotherapy. Non‐apoptotic death mode of ferroptosis has been regarded as a new strategy to enhance tumor immunotherapy against drug‐resistant cancers. The lethal accumulation of lipid peroxides (LPO) determines the progress of ferroptosis. The high susceptibleness of ferroptosis provides an opportunity for combating triple‐negative breast cancer. Reactive nitrogen species (RNS) produced by nitric oxide (NO) and reactive oxygen species (ROS) is more lethal than ROS for tumor cells. Herein, an RNS‐mediated immunotherapy strategy for inducing ferroptosis pathway is proposed by improving LPO accumulation, and constructed a multifunctional liposome (Lipo‐MT‐SNAP) comprised of peroxynitrite (ONOO−) generator, tumor targeted group, inhibiting glutathione peroxidase 4 (GPX4), and basic units (dipalmitoyl phosphatidylcholine and cholesterol). The significant enhancement of LPO resulted from the intense oxidative damage of ONOO− impaired synthesis of GPX4 by depleting glutathione, which further amplified ferroptosis and triggered immunogenic cell death. In vivo, RNS‐mediated photoimmunotherapy can promote polarization of M2 to M1 macrophages and dendritic cells maturation, further infiltrate T cells, regulate the secretion of inflammatory factors, and reprogram the tumor microenvironment. The powerful RNS‐mediated ferroptosis induces strong immunogenicity and effectively inhibit tumor proliferation.
Electron‐Spin Regulation Driving Heterointerface Electron Distribution and Phase Transition toward Ultrafast and Durable Sodium Storage
Authors: Li Yuhang , Li Wenying , Zhang Meng , Zhuang Yanhui , Li Huaidong , Pan Zhigang , Min Huihua , Chen Tsung‐Yi , Chen Han‐Yi , Yang Hao , Wang Jin 
Published: September 2024

AbstractPhase engineering is an effective strategy for modulating the electronic structure and electron transfer mobility of cobalt selenide (CoSe2) with remarkable sodium storage. Nevertheless, it remains challenging to improve fast‐charging and cycling performance. Herein, a heterointerface coupling induces phase transformation from cubic CoSe2 to orthorhombic CoSe2 accompanied by the formation of MoSe2 to construct a CoSe2/MoSe2 heterostructure decorated with N‐doped carbon layer on a 3D graphene foam (CoSe2/MoSe2@NC/GF). The incorporated Mo cations in the bridged o‐CoSe2/MoSe2 not only act an electron donor to regulate charge‐spin configurations with more active electronic states but also trigger the upshift of d/p band centers and a decreased ∆d‐p band center gap, which greatly enhances ion adsorption capability and lowers the ion diffusion barrier. As expected, the CoSe2/MoSe2@NC/GF anode demonstrates a high‐rate capability of 447 mAh g−1 at 2 A g−1 and an excellent cyclability of 298 mAh g−1 at 1 A g−1 over 1000 cycles. The work deepens the understanding of the elaborate construction of heterostructured electrodes for high‐performance SIBs.
Harvesting Green Hydrogen from the Deep Blue: Seawater‐Compatible SnSe‐P Decorated Graphene‐CNTs Based Electrocatalyst Under Universal pH
Authors: Pahuja Mansi , Dastider Saptarshi Ghosh , Jyoti  , Alam Kehkashan , Rani Seema , Das Subhabrata , Urkude Rajashri , Afshan Mohd , Rani Daya , Chaudhary Nikita , Siddiqui Shumile Ahmed , Riyajuddin SK , Ghosh Rishita , Mondal Krishnakanta , Ghosh Kaushik 
Published: September 2024

AbstractFabrication of cost‐effective and robust metal‐based electrocatalysts for hydrogen evolution reactions (HER) across the entire pH range has garnered significant attention in harvesting renewable energy. Herein, the fabrication of 3D high‐surface Ni Foam‐Graphene‐Carbon Nanotubes (NGC) decorated with phosphorous‐inserted tin selenide (SnSe‐P) showcases unprecedented HER activity with minimal overpotentials across all pH ranges (52 mV in acidic, 93 mV in basic, and 198 mV in neutral conditions@10 mA cm−2) and stability at 1 A cm−2 for 72 h. The as‐designed catalyst shows a low overpotential of 122 mV@10 mA cm−2 in alkaline seawater, achieved through controlled electronic distribution on Sn site after incorporation of P in NGC‐SnSe‐P. A stable cell voltage of 1.56 V@10 mA cm⁻2 is achieved for prolonged time in 1 m KOH toward overall water electrolysis. Experimental and theoretical investigation reveals that the insertion of P in layered SnSe enables s orbitals of H* and p orbitals of Sn to interact, favoring the adsorption of the H* intermediate. A renewable approach is adopted by using silicon solar cells (η = 10.66%) to power up the electrolyzer, yielding a solar‐to‐hydrogen (STH) conversion efficiency of 7.70% in 1 m KOH and 5.65% in alkaline seawater, aiming toward green hydrogen production.