bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sequential Coalescence Enabled Two‐Step Microreactions in Triple‐Core Double‐Emulsion Droplets Triggered by an Electric Field
Authors: Jia Yankai , Ren Yukun , Hou Likai , Liu Weiyu , Deng Xiaokang , Jiang Hongyuan 
Published: October 2017

AbstractAdvances in microfluidic emulsification have enabled the generation of exquisite multiple‐core droplets, which are promising structures to accommodate microreactions. An essential requirement for conducting reactions is the sequential coalescence of the multiple cores encapsulated within these droplets, therefore, mixing the reagents together in a controlled sequence. Here, a microfluidic approach is reported for the conduction of two‐step microreactions by electrically fusing three cores inside double‐emulsion droplets. Using a microcapillary glass device, monodisperse water‐in‐oil‐in‐water droplets are fabricated with three compartmented reagents encapsulated inside. An AC electric field is then applied through a polydimethylsiloxane chip to trigger the sequential mixing of the reagents, where the precise sequence is guaranteed by the discrepancy of the volume or conductivity of the inner cores. A two‐step reaction in each droplet is ensured by two times of core coalescence, which totally takes 20–40 s depending on varying conditions. The optimal parameters of the AC signal for the sequential fusion of the inner droplets are identified. Moreover, the capability of this technique is demonstrated by conducting an enzyme‐catalyzed reaction used for glucose detection with the double‐emulsion droplets. This technique should benefit a wide range of applications that require multistep reactions in micrometer scale.
Learning from ancient water management: Archeology's role in modern‐day climate change adaptations
Authors: Kaptijn Eva 
Published: October 2017

Climate change is altering our environment and societies worldwide have to devise adaptation strategies. Water management strategies are becoming especially important. In the past, societies had to adapt in order to survive as well. Communities often practised long‐term sustainable agriculture. By understanding the ways in which ancient communities were successful at or failed in attaining social‐ecological resilience through water management archeologists can provide important information for modern communities facing similar problems. Archeology's long time perspective is very valuable. However, archeologists are confronted with a number of issues. Archeology can only study the material remains of past societies, not the living communities. Not all human activity translates into material residue and not all materials survive. Moreover, people will not demonstrate completely rational cause‐and‐effect behavior, but ideology and beliefs, which archeology can only poorly attest, will also have influenced decisions. Nevertheless, archeology can bring a unique perspective to the debate on climate change adaptation: archeology can falsify or corroborate sustainability claims, ancient water management techniques may still be a resilient mode of subsistence and ancient techniques often rely on relatively simple technology allowing for easier adoption. When transposing ancient water management techniques to modern situations it is important to involve stakeholders from an early stage, to incorporate traditional knowledge systems as much as possible and most importantly to ascertain whether physical and socio‐cultural circumstances are comparable. Archeological knowledge on ancient sustainability and water management is not a panacea for all climate related aridification, but can contribute a unique longue durée perspective. WIREs Water 2018, 5:e1256. doi: 10.1002/wat2.1256This article is categorized under:

Human Water > Water as Imagined and Represented
On the origins and industrial applications of Saccharomyces cerevisiae × Saccharomyces kudriavzevii hybrids
Authors: Peris David , Pérez‐Torrado Roberto , Hittinger Chris Todd , Barrio Eladio , Querol Amparo 
Published: October 2017

AbstractCompanies based on alcoholic fermentation products, such as wine, beer and biofuels, use yeasts to make their products. Each industrial process utilizes different media conditions, which differ in sugar content, the presence of inhibitors and fermentation temperature. Saccharomyces cerevisiae has traditionally been the main yeast responsible for most fermentation processes. However, the market is changing due to consumer demand and external factors such as climate change. Some processes, such as biofuel production or winemaking, require new yeasts to solve specific challenges, especially those associated with sustainability, novel flavours and altered alcohol content. One of the proposed solutions is the application of yeast hybrids. The lager beer market has been dominated by S. cerevisiae × S. eubayanus hybrids. However, several less thoroughly studied hybrids have been isolated from other diverse industrial processes. Here we focus on S. cerevisiae × S. kudriavzevii hybrids, which have been isolated from diverse industrial conditions that include wine, ale beer, cider and dietary supplements. Emerging data suggest an extended and complex story of adaptation of these hybrids to traditional industrial conditions. S. cerevisiae × S. kudriavzevii hybrids are also being explored for new industrial applications, such as biofuels. This review describes the past, present and future of S. cerevisiae × S. kudriavzevii hybrids. Copyright © 2017 John Wiley & Sons, Ltd.
A Trichosporonales genome tree based on 27 haploid and three evolutionarily conserved ‘natural’ hybrid genomes
Authors: Takashima Masako , Sriswasdi Sira , Manabe Ri‐Ichiroh , Ohkuma Moriya , Sugita Takashi , Iwasaki Wataru 
Published: October 2017

AbstractTo construct a backbone tree consisting of basidiomycetous yeasts, draft genome sequences from 25 species of Trichosporonales (Tremellomycetes, Basidiomycota) were generated. In addition to the hybrid genomes of Trichosporon coremiiforme and Trichosporon ovoides that we described previously, we identified an interspecies hybrid genome in Cutaneotrichosporon mucoides (formerly Trichosporon mucoides). This hybrid genome had a gene retention rate of ~55%, and its closest haploid relative was Cutaneotrichosporon dermatis. After constructing the C. mucoides subgenomes, we generated a phylogenetic tree using genome data from the 27 haploid species and the subgenome data from the three hybrid genome species. It was a high‐quality tree with 100% bootstrap support for all of the branches. The genome‐based tree provided superior resolution compared with previous multi‐gene analyses. Although our backbone tree does not include all Trichosporonales genera (e.g. Cryptotrichosporon), it will be valuable for future analyses of genome data. Interest in interspecies hybrid fungal genomes has recently increased because they may provide a basis for new technologies. The three Trichosporonales hybrid genomes described in this study are different from well‐characterized hybrid genomes (e.g. those of Saccharomyces pastorianus and Saccharomyces bayanus) because these hybridization events probably occurred in the distant evolutionary past. Hence, they will be useful for studying genome stability following hybridization and speciation events. Copyright © 2017 John Wiley & Sons, Ltd.
Evaluation of Prognostic Factors for Esophageal Squamous Cell Carcinoma Treated with Neoadjuvant Chemoradiotherapy Followed by Surgery
Authors: Hamai Yoichi , Hihara Jun , Emi Manabu , Furukawa Takaoki , Murakami Yuji , Nishibuchi Ikuno , Ibuki Yuta , Yamakita Ichiko , Kurokawa Tomoaki , Nagata Yasushi , Okada Morihito 
Published: October 2017

AbstractBackgroundIntensive trimodality therapy is needed for locally advanced esophageal squamous cell carcinoma (ESCC). However, some patients develop recurrence and die of cancer even after trimodality therapy.MethodsWe evaluated prognostic factors based on data from 125 patients with ESCC who underwent neoadjuvant chemoradiotherapy (NCRT) comprising concurrent chemotherapy and 40 Gy of radiation, followed by curative‐intent esophagectomy.ResultsThirty‐four (27.2%) patients achieved a pathological complete response (pCR) after NCRT. The 5‐year recurrence‐free (RFS) and overall survival (OS) rates of all patients were 49.2 and 52.9%, respectively, and were significantly better for patients with pCR than without pCR (p = 0.01 and 0.02, respectively). Univariate and multivariate analyses selected performance status [PS 0 vs. 1: hazard ratio (HR) 2.05; 95% confidence interval (CI) 1.30–4.84; p = 0.01] and ypN (0 vs. 1: HR 2.33; 95% CI 1.12–4.84; p = 0.02; 0 vs. 2/3: HR 3.73; 95% CI 1.68–8.28; p = 0.001) as independent covariates for RFS. Furthermore, PS (0 vs. 1; HR 2.94; 95% CI 1.51–5.72; p = 0.002) and ypN (0 vs. 1; HR 2.26; 95% CI 1.09–4.69; p = 0.03; 0 vs. 2/3: HR 3.90; 95% CI 1.79–8.48; p = 0.001) were also independent covariates for OS.ConclusionsPerformance status 1 and ypN+ were significantly associated with a poor prognosis after trimodality therapy for ESCC.
Carnosinase, diabetes mellitus and the potential relevance of carnosinase deficiency
Authors: Peters Verena , Zschocke Johannes , Schmitt Claus P. 
Published: October 2017

AbstractCarnosinase (CN1) is a dipeptidase, encoded by the CNDP1 gene, that degrades histidine‐containing dipeptides, such as carnosine, anserine and homocarnosine. Loss of CN1 function (also called carnosinase deficiency or aminoacyl‐histidine dipeptidase deficiency) has been reported in a small number of patients with highly elevated blood carnosine concentrations, denoted carnosinaemia; it is unclear whether the variety of clinical symptoms in these individuals is causally related to carnosinase deficiency. Reduced CN1 function should increase serum carnosine concentrations but the genetic basis of carnosinaemia has not been formally confirmed to be due to CNDP1 mutations. A CNDP1 polymorphism associated with low CN1 activity correlates with significantly reduced risk for diabetic nephropathy, especially in women with type 2 diabetes, and may slow progression of chronic kidney disease in children with glomerulonephritis. Studies in rodents demonstrate antiproteinuric and vasculoprotective effects of carnosine, the precise molecular mechanisms, however, are still incompletely understood. Thus, carnosinemia due to CN1 deficiency may be a non‐disease; in contrast, carnosine may potentially protect against long‐term sequelae of reactive metabolites accumulating, e.g. in diabetes and chronic renal failure.
Emigration Rates From Sample Surveys: An Application to Senegal
Authors: Willekens Frans , Zinn Sabine , Leuchter Matthias 
Published: October 2017

AbstractWhat is the emigration rate of a country, and how reliable is that figure? Answering these questions is not at all straightforward. Most data on international migration are census data on foreign-born population. These migrant stock data describe the immigrant population in destination countries but offer limited information on the rate at which people leave their country of origin. The emigration rate depends on the number leaving in a given period and the population at risk of leaving, weighted by the duration at risk. Emigration surveys provide a useful data source for estimating emigration rates, provided that the estimation method accounts for sample design. In this study, emigration rates and confidence intervals are estimated from a sample survey of households in the Dakar region in Senegal, which was part of the Migration between Africa and Europe survey. The sample was a stratified two-stage sample with oversampling of households with members abroad or return migrants. A combination of methods of survival analysis (time-to-event data) and replication variance estimation (bootstrapping) yields emigration rates and design-consistent confidence intervals that are representative for the study population.
Size, shape, and topology optimization of planar and space trusses using mutation-based improved metaheuristics
Authors: Tejani Ghanshyam G. , Savsani Vimal J. , Patel Vivek K. , Savsani Poonam V. 
Published: October 2017

Abstract
In this study, simultaneous size, shape, and topology optimization of planar and space trusses are investigated. Moreover, the trusses are subjected to constraints for element stresses, nodal displacements, and kinematic stability conditions. Truss Topology Optimization (TTO) removes the superfluous elements and nodes from the ground structure. In this method, the difficulties arise due to unacceptable and singular topologies; therefore, the Grubler's criterion and the positive definiteness are used to handle such issue. Moreover, the TTO is challenging due to its search space, which is implicit, non-convex, non-linear, and often leading to divergence. Therefore, mutation-based metaheuristics are proposed to investigate them. This study compares the performance of four improved metaheuristics (viz. Improved Teaching–Learning-Based Optimization (ITLBO), Improved Heat Transfer Search (IHTS), Improved Water Wave Optimization (IWWO), and Improved Passing Vehicle Search (IPVS)) and four basic metaheuristics (viz. TLBO, HTS, WWO, and PVS) in order to solve structural optimization problems.
Highlights Improvements in four recently designed metaheuristics. Use of random mutation-based search technique. Applications on challenging/benchmark problems of simultaneous size, shape, and topology optimization of truss structures. Improvements effective over basic metaheuristics.
Legal Consciousness and Workers’ Resistance in Đồng Nai Province, Vietnam
Authors: NGUYEN Tu Phuong 
Published: October 2017

AbstractThis article examines how labour law contributes to labour resistance in Vietnam through an empirical case study of the ‘core workers’ in Đồng Nai Province. These core workers are factory workers who have undergone legal training and who provide legal aid to factory workers in need. They have, at the same time, deployed their legal knowledge to demand access to justice for themselves and the factory workers. This article demonstrates that the core workers’ legal consciousness is shaped by their mobilization of the law and their own workplace experiences. It then investigates in detail a core worker’s engagements with individual and collective disputes, and discusses his views on legal aid, labour law, and workplace relationships. This article argues that the core workers’ resistance is not only a fight against illegal practices, but that it also embodies a call for the management’s moral obligations towards its workers.
Two glutathione transferase isoforms isolated from juvenile cysts ofTaenia crassiceps: identification, purification and characterization
Authors: Maldonado G. , Nava G. , Plancarte A. 
Published: October 2017

AbstractWe identified and characterized the first two glutathione transferases (GSTs) isolated from juvenile cysts ofTaenia crassiceps(EC 2.5.1.18). The two glutathione transferases (TcGST1 and TcGST2) were purified in a single-step protocol using glutathione (GSH)-sepharose chromatography in combination with a GSH gradient. The specific activities of TcGST1 and TcGST2 were 26 U mg−1and 19 U mg−1, respectively, both at 25°C and pH 6.5 with 1-chloro-2,4-dinitrobenzene (CDNB) and GSH as substrates. TheKm(CDNB)andKcat(CDNB)values for TcGST1 and TcGST2 (0.86 μmand 62 s−1; 1.03 μmand 1.97 s−1, respectively) andKm(GSH)andKcat(GSH)values for TcGST1 and TcGST2 (0.55 μmand 11.61 s−1; 0.3 μmand 32.3 s−1, respectively) were similar to those reported for mammalian and helminth GSTs. Mass spectrometry analysis showed that eight peptides from each of the two parasite transferases were a match for gi|29825896 glutathione transferase (Taenia solium), confirming that both enzymes are GSTs. The relative molecular masses were 54,000 ± 0.9 for the native enzymes and 27,500 ± 0.5 for the enzyme subunits. Thus, TcGST1 and TcGST2 are dimeric proteins. Optimal TcGST1 and TcGST2 activities were observed at pH 8.5 in the range of 20–55°C and pH 7.5 at 35–40°C, respectively. TcGST1 and TcGST2 were inhibited by cibacron blue (CB), bromosulphophthalein (BST), rose bengal (RB), indomethacin and haematin (Hm) with 50% inhibitory concentrations (IC50) in the μmrange. TcGST1 was inhibited in a non-competitive manner by all tested inhibitors with the exception of indomethacin, which was uncompetitive. The discovery of these new GSTs facilitates the potential use ofT. crassicepsas a model to investigate multifunctional GSTs.
Buying through your teeth: traditional currency and conservation of flying foxesPteropusspp. in Solomon Islands
Authors: Lavery Tyrone H. , Fasi John 
Published: October 2017

AbstractGlobally, island bats are vulnerable to subsistence hunting, with widespread population declines, local extirpations and extinctions. Bats are important to the ecological functioning of remote oceanic islands, and thus the sustainable management of hunting of flying foxesPteropusspp. is a conservation priority in the Pacific. In Solomon Islands people hunt flying foxes for bushmeat and their canine teeth, which are used as traditional currency. The value of teeth potentially increases hunting pressure on species ofPteropus. We interviewed 197 people on Makira Island to determine the nature of this use and how it may influence flying-fox populations. We asked questions to gather information about hunting practices, the value of canine teeth, utilization of traditional currency, and population trends ofPteropus. Flying-fox teeth remain highly valued on Makira. It is primarily the teeth ofP. tonganusthat are used rather than those of the smallerP. cognatus. Although flying foxes are not targeted solely for their teeth, this added value seemingly drives hunters to focus onP. tonganus. Hunting varied across five regions of Makira and high hunting pressure corresponded with perceived rarity ofP. tonganus. Regions with low hunting pressure may be opportune locations to initiate flying-fox conservation on Makira.
The effects of snow and salt on ice table stability in University Valley, Antarctica
Authors: Williams K.E. , Heldmann J.L. , McKay Christopher P. , Mellon Michael T. 
Published: October 2017

AbstractThe Antarctic Dry Valleys represent a unique environment where it is possible to study dry permafrost overlaying an ice-rich permafrost. In this paper, two opposing mechanisms for ice table stability in University Valley are addressed: i) diffusive recharge via thin seasonal snow deposits and ii) desiccation via salt deposits in the upper soil column. A high-resolution time-marching soil and snow model was constructed and applied to University Valley, driven by meteorological station atmospheric measurements. It was found that periodic thin surficial snow deposits (observed in University Valley) are capable of drastically slowing (if not completely eliminating) the underlying ice table ablation. The effects of NaCl, CaCl2 and perchlorate deposits were then modelled. Unlike the snow cover, however, the presence of salt in the soil surface (but no periodic snow) results in a slight increase in the ice table recession rate, due to the hygroscopic effects of salt sequestering vapour from the ice table below. Near-surface pore ice frequently forms when large amounts of salt are present in the soil due to the suppression of the saturation vapour pressure. Implications for Mars high latitudes are discussed.
Neuropsychological Recovery Trajectories in Moderate to Severe Traumatic Brain Injury: Influence of Patient Characteristics and Diffuse Axonal Injury
Authors: Rabinowitz Amanda R. , Hart Tessa , Whyte John , Kim Junghoon 
Published: October 2017

AbstractObjectives: The goal of the present study was to elucidate the influence of demographic and neuropathological moderators on the longitudinal trajectory neuropsychological functions during the first year after moderate to severe traumatic brain injury (TBI). In addition to examining demographic moderators such as age and education, we included a measure of whole-brain diffuse axonal injury (DAI), and examined measures of processing speed (PS), executive function (EF), and verbal learning (VL) separately. Methods: Forty-six adults with moderate to severe TBI were examined at 3, 6, and 12 months post-injury. Participants underwent neuropsychological evaluation and neuroimaging including diffusion tensor imaging. Using linear mixed effects modeling, we examined longitudinal trajectories and moderating factors of cognitive outcomes separately for three domains: PS, VL, and EF. Results: VL and EF showed linear improvements, whereas PS exhibited a curvilinear trend characterized by initial improvements that plateaued or declined, depending on age. Age moderated the recovery trajectories of EF and PS. Education and DAI did not influence trajectory but were related to initial level of functioning for PS and EF in the case of DAI, and all three cognitive domains in the case of education. Conclusions: We found disparate recovery trajectories across cognitive domains. Younger age was associated with more favorable recovery of EF and PS. These findings have both clinical and theoretical implications. Future research with a larger sample followed over a longer time period is needed to further elucidate the factors that may influence cognitive change over the acute to chronic period after TBI. (JINS, 2018, 24, 237–246)
Russian Federation – Measures on the Importation of Live Pigs, Pork and Other Pig Products from the European Union (Russia–Pigs (EU)), DS475
Authors: Alcover Maria 
Published: October 2017

This dispute arose after African Swine Fever (ASF), a highly contagious disease of pigs, was detected in four member States of the European Union (EU) and Russia stopped accepting imports of pigs and pig products from the EU. The EU presented claims with respect to two measures: (i) the ‘EU-wide ban’, consisting of Russia's ban on the importation of the products at issue from the entire EU; and (ii) the ‘country-specific import bans’ imposed by Russia on imports from the four EU member States – Estonia, Latvia, Lithuania, and Poland. There were two categories of products at issue: ‘treated products’ (finished products subject to a treatment that ensures destruction of ASF) and ‘non-treated products’ (products including live pigs, and raw meat preparations). Treated products were not subject to the EU-wide ban. The EU brought multiple claims under the Agreement on the Application of Sanitary and Phytosanitary Measures (SPS Agreement).
Cyanophage-encoded lipid desaturases: oceanic distribution, diversity and function
Authors: Roitman Sheila , Hornung Ellen , Flores-Uribe José , Sharon Itai , Feussner Ivo , Béjà Oded 
Published: October 2017

Abstract
Cyanobacteria are among the most abundant photosynthetic organisms in the oceans; viruses infecting cyanobacteria (cyanophages) can alter cyanobacterial populations, and therefore affect the local food web and global biochemical cycles. These phages carry auxiliary metabolic genes (AMGs), which rewire various metabolic pathways in the infected host cell, resulting in increased phage fitness. Coping with stress resulting from photodamage appears to be a central necessity of cyanophages, yet the overall mechanism is poorly understood. Here we report a novel, widespread cyanophage AMG, encoding a fatty acid desaturase (FAD), found in two genotypes with distinct geographical distribution. FADs are capable of modulating the fluidity of the host’s membrane, a fundamental stress response in living cells. We show that both viral FAD (vFAD) families are Δ9 lipid desaturases, catalyzing the desaturation at carbon 9 in C16 fatty acid chains. In addition, we present a comprehensive fatty acid profiling for marine cyanobacteria, which suggests a unique desaturation pathway of medium- to long-chain fatty acids no longer than C16, in accordance with the vFAD activity. Our findings suggest that cyanophages are capable of fiddling with the infected host’s membranes, possibly leading to increased photoprotection and potentially enhancing viral-encoded photosynthetic proteins, resulting in a new viral metabolic network.