bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microdiversity of Echinococcus granulosus sensu stricto in Australia
Authors: ALVAREZ ROJAS C. A. , EBI D. , Gauci C. G. , SCHEERLINCK J. P. , WASSERMANN M. , JENKINS D. J. , LIGHTOWLERS M. W. , ROMIG T. 
Published: April 2016

SUMMARYEchinococcus granulosus (sensu lato) is now recognized as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. One of these species, E. granulosus sensu stricto (s.s.), is now clearly identified as the principal agent causing cystic echinococcosis in humans. Previous studies of a small section of the cox1 and nadh1 genes identified two variants of E. granulosus s.s. to be present in Australia; however, no further work has been carried out to characterize the microdiversity of the parasite in its territory. We have analysed the sequence of the full length of the cox1 gene (1609 bp) from 37 isolates of E. granulosus from different hosts and geographic regions of Australia. The analysis shows that seven haplotypes of E. granulosus s.s. not previously described were found, together with five haplotypes known to be present in other parts of the world, including the haplotype EG01 which is widespread and present in all endemic regions. These data extend knowledge related to the geographical spread and host range of E. granulosus s.s. in a country such as Australia in which the parasite established around 200 years ago.
Tetractinomyxon stages genetically consistent withSphaerospora dicentrarchi(Myxozoa: Sphaerosporidae) found inCapitellasp. (Polychaeta: Capitellidae) suggest potential role of marine polychaetes in parasite's life cycle
Authors: RANGEL LUIS F. , CASTRO RICARDO , ROCHA SÓNIA , SEVERINO RICARDO , CASAL GRAÇA , AZEVEDO CARLOS , CAVALEIRO FRANCISCA , SANTOS MARIA J. 
Published: April 2016

SUMMARYKnown life cycles of myxosporean parasites have two hosts, but very few life cycles have been disclosed, especially in the marine environment.Sphaerospora dicentrarchiSitjà-Bobadilla and Álvarez-Pellitero, 1992 is a systemic parasite from the European seabass,Dicentrarchus labrax(Linnaeus, 1758), a highly valuable commercial fish. It affects its health, leading to aquaculture production losses. During 2013 and 2014, an actinospore survey was conducted in a total of 5942 annelids collected from a fish farm in Algarve and from the Aveiro Estuary, in Portugal. A new tetractinomyxon actinospore was found in a capitellid polychaete, belonging to the generaCapitellacollected at the fish farm. The tetractinomyxons were pyriform measuring 11·1 ± 0·7µm in length and 7·2 ± 0·4µm in width, and presented three rounded polar capsules measuring 2·4 ± 0·3µm in diameter. The molecular analysis of the 18S rRNA gene sequences from the tetractinomyxons revealed a similarity of 100% with the DNA sequences deposited in the GenBank fromS. dicentrarchimyxospores collected from the European seabass and the spotted seabass in the same fish farm and 99·9% similarity with the DNA sequence obtained from the myxospores found infecting the European seabass in the Aveiro Estuary. Therefore, the new tetractinomyxons are inferred to represent the actinospore phase of theS. dicentrarchilife cycle.
The diversity–disease relationship: evidence for and criticisms of the dilution effect
Authors: HUANG Z. Y. X. , VAN LANGEVELDE F. , ESTRADA-PEÑA A. , SUZÁN G. , DE BOER W. F. 
Published: April 2016

SUMMARYThe dilution effect, that high host species diversity can reduce disease risk, has attracted much attention in the context of global biodiversity decline and increasing disease emergence. Recent studies have criticized the generality of the dilution effect and argued that it only occurs under certain circumstances. Nevertheless, evidence for the existence of a dilution effect was reported in about 80% of the studies that addressed the diversity–disease relationship, and a recent meta-analysis found that the dilution effect is widespread. We here review supporting and critical studies, point out the causes underlying the current disputes. The dilution is expected to be strong when the competent host species tend to remain when species diversity declines, characterized as a negative relationship between species’ reservoir competence and local extinction risk. We here conclude that most studies support a negative competence–extinction relationship. We then synthesize the current knowledge on how the diversity–disease relationship can be modified by particular species in community, by the scales of analyses, and by the disease risk measures. We also highlight the complex role of habitat fragmentation in the diversity–disease relationship from epidemiological, evolutionary and ecological perspectives, and construct a synthetic framework integrating these three perspectives. We suggest that future studies should test the diversity–disease relationship across different scales and consider the multiple effects of landscape fragmentation.
Analysis of the transmission ofTrypanosoma cruziinfection through hosts and vectors
Authors: FABRIZIO MARÍA C. , SCHWEIGMANN NICOLÁS J. , BARTOLONI NORBERTO J. 
Published: April 2016

SUMMARYCalculating epidemiological measures of infection byTrypanosoma cruzi, the causative agent of Chagas disease, is complex, because it involves several species, different stages of infection in humans and multiple transmission routes. Using the next-generation matrix method, we analysed a model which considers the three stages of human infection, triatomines and dogs (the main domestic reservoirs ofT. cruziwhen triatomines are present) and the main transmission routes. We derivedR0and type-reproduction numbersT.We deduced formulas for the number of new infections generated through each transmission route by each infected individual. We applied our findings in Argentine Gran Chaco. The expressions achieved allowed quantifying the high infectivity of dogs and emphasizing the epidemiological importance of the long and asymptomatic chronic indeterminate stage in humans in the spread of the infection. According to the model, it is expected that one infected human infects 21 triatomines, that 100 infected triatomines are necessary to infect one human and 34 to infect a dog, and that each dog infects on average one triatomine per day. Our results may allow quantifying the effect of control measures on infected humans, triatomines and dogs (or other highly infected vertebrate) or on a specific route of transmission, in other scenarios.
Continental Potters? First-Century Roman Flagon Production at Duxford, Cambridgeshire
Authors: Anderson Katie , Woolhouse Tom , Marter-Brown Kayt , Quinn Patrick 
Published: April 2016

AbstractExcavations in Duxford, Cambridgeshire, in 2013, revealed six early Roman (a.d.50–80) pottery kilns. The kilns were used for the production of flagons, specifically collared and ring-necked varieties. Flagons are generally scarce in contemporary domestic assemblages in Cambridgeshire, often only occurring in ‘special’ contexts, such as burials, while collared flagons are closely associated with military consumption. The excavations also produced a large, significant assemblage of perforated kiln plates. The technology and repertoire of vessels suggest that manufacture was conducted by non-local potters for a specialist market. The site forms part of a group of other early Roman kiln sites in the Cambridge environs and adds to the growing picture of pottery production in the decades following the Roman Conquest.
Immunological and histochemical analyses of cerebrospinal fluid and peripheral blood from patients with neurological and psychiatric disorders
Authors: Maxeiner Horst-G. , Rojewski Markus Thomas , Tumani Hayrettin , Herzog Sibylle , Fuchs Dietmar , Schmitt Anita , Schmitt Michael , Bechter Karl 
Published: April 2016

Abstract:Epidemiological, clinical and post mortem studies indicate that inflammatory and immune reactions are involved in the pathomechanisms of affective and schizophrenic spectrum disorders. However, in psychiatric patients, only sporadic investigation on immunochemistry has been performed and information about immunofunction derived by investigation of immunocompetent cells in the CSF is not available to date.Here we present an interdisciplinary work of neurologists, psychiatrists and hemato-immunologists focusing on the immunology of psychiatric and neurological disorders. In a first study including 63 patients with therapy resistant affective and schizophrenic spectrum disorders we applied conventional, validated neurological CSF investigation such as analysis of albumin, IgG, IgA, IgM, oligoclonal IgG and specific antibodies, cell count and interpreted the data by Reibergrams.In a second study, we applied the highly sensitive and specific multicolour flowcytometry of paired samples of CSF and peripheral blood cells to characterize the immunostatus of psychiatric and neurological patients. We demonstrate that flowcytometry technology constitutes an appropriate method to investigate subsets of lymphocytes even with low CSF cell numbers, and therefore as a promising diagnostic tool for routine purposes in the differential diagnosis of psychiatric diseases. Furthermore, knowledge of the frequencies of T cell subsets such as the T regulatory cell type might open new avenues to models of psychiatric and neurological diseases as well as diagnostic and monitoring implications.
Maternal mental well-being during pregnancy and glucocorticoid receptor gene promoter methylation in the neonate
Authors: Mansell Toby , Vuillermin Peter , Ponsonby Anne-Louise , Collier Fiona , Saffery Richard ,   Barwon Infant Study Investigator Team, Ryan Joanne 
Published: April 2016

AbstractMaternal mental health during pregnancy has been linked to health outcomes in progeny. Mounting evidence implicates fetal “programming” in this process, possibly via epigenetic disruption. Maternal mental health has been associated with glucocorticoid receptor methylation (nuclear receptor subfamily 3, group C, member 1 [NR3C1]) in the neonate; however, most studies have been small (n < 100) and have failed to control for multiple testing in the statistical analysis. The Barwon Infant Study is a population-derived birth cohort with antenatal recruitment. Maternal depression and anxiety were assessed using the Edinburgh Postnatal Depression Scale and psychological distress using the Perceived Stress Scale. NR3C1 cord blood methylation levels were determined using Sequenom MassArray for 481 participants. Maternal psychological distress and anxiety were associated with a small increase in neonate NR3C1 methylation at specific CpG sites, thus replicating some previous findings. However, associations were only nominally significant and did not remain after correction for the number of CpG sites and exposures investigated. As the largest study to explore the relationship between maternal well-being and offspring NR3C1 cord blood methylation, our results highlight the need for caution when interpreting previous findings in this area. Future studies must ensure they are adequately powered to detect the likely small effect sizes while controlling for multiple testing.
Differences in quantitative methods for measuring subjective cognitive decline – results from a prospective memory clinic study
Authors: Vogel Asmus , Salem Lise Cronberg , Andersen Birgitte Bo , Waldemar Gunhild 
Published: April 2016

ABSTRACTBackground:Cognitive complaints occur frequently in elderly people and may be a risk factor for dementia and cognitive decline. Results from studies on subjective cognitive decline are difficult to compare due to variability in assessment methods, and little is known about how different methods influence reports of cognitive decline.Methods:The Subjective Memory Complaints Scale (SMC) and The Memory Complaint Questionnaire (MAC-Q) were applied in 121 mixed memory clinic patients with mild cognitive symptoms (mean MMSE = 26.8, SD 2.7). The scales were applied independently and raters were blinded to results from the other scale. Scales were not used for diagnostic classification. Cognitive performances and depressive symptoms were also rated. We studied the association between the two measures and investigated the scales’ relation to depressive symptoms, age, and cognitive status.Results:SMC and MAC-Q were significantly associated (r = 0.44, N = 121, p = 0.015) and both scales had a wide range of scores. In this mixed cohort of patients, younger age was associated with higher SMC scores. There were no significant correlations between cognitive test performances and scales measuring subjective decline. Depression scores were significantly correlated to both scales measuring subjective decline. Linear regression models showed that age did not have a significant contribution to the variance in subjective memory beyond that of depressive symptoms.Conclusions:Measures for subjective cognitive decline are not interchangeable when used in memory clinics and the application of different scales in previous studies is an important factor as to why studies show variability in the association between subjective cognitive decline and background data and/or clinical results. Careful consideration should be taken as to which questions are relevant and have validity when operationalizing subjective cognitive decline.
What Have We Learned? Needs Assessment
Authors: Deitchman Scott 
Published: April 2016

AbstrtactThis is a synthesis of the presentations and discussions pertaining to Needs Assessments of the Conference, Health Aspects of the Tsunami Disaster in Asia, convened by the World Health Organization (WHO), in Phuket, Thailand, 04–06 May 2005. The topics discussed included: (1) Needs assessments; (2) Coordination; (3) Effectiveness of needs assessments; (4) Needs assessments change; (5) Qualifications for conducting needs assessments; (6) Dissemination; (7) Emergencies overlay background needs assessments; (8) Need for standards; and (9) Conclusions and recommendations.
Neuroimaging and the Neuroanatomy of Posttraumatic Stress Disorder
Authors: Rauch Scott L. , Shin Lisa M. , Whalen Paul J. , Pitman Roger K. 
Published: April 2016

AbstractContemporary neuroimaging methods have been used to gather initial data regarding the neural substrates of posttraumatic stress disorder (PTSD). Morphometric magnetic resonance imaging (MRI) studies have reliably shown reduced hippocampal volume in subjects with PTSD vs control cohorts. Functional imaging studies have implicated a network of brain regions in PTSD, comprising the amygdala, hippocampus, and anterior paralimbic territories (including anterior cingulate cortex), as well as Broca's area and visual cortex. Extant relevant neuroimaging data are reviewed, and a tentative heuristic neuroanatomical model of PTSD is provided. In conclusion, emerging strategies for advancement in this field are outlined.
Individual emergency physician admission rates: predictably unpredictable
Authors: Mutrie David , Bailey S. Kathleen , Malik Saleem 
Published: April 2016

ABSTRACTObjective:We sought to determine the degree and possible causes of variability in admission practices among individual emergency physicians (EPs) at 1 emergency department (ED) using a Canadian Emergency Department Triage Acuity Scale (CTAS)–matched ED patient population.Methods:We distributed a survey measuring attitudes and demographics to all EPs (n= 30) at a large regional hospital. Hospital admissions data from 1 calendar year were matched to individual EP survey results. Emergency physicians were ranked as “lower,” “average” or “higher” admitters and, using these categorical variables, the data set was analyzed for correlations and trends.Results:Overall, 97.0% of the EPs responded to the survey. Admissions by EPs ranged from 8.7% to 17.0%, (mean 12.52, standard deviation [SD] 2.21) of all patients seen. CTAS category–specific admission data demonstrated variability in the admission ranking of individual EPs. No EPs consistently performed at any 1 admission ranking across all CTAS categories. More years of emergency medicine experience was significantly correlated with higher admissions in the CTAS-2 ranking (r= 0.4,p&lt; 0.05). Whether a physician worked full-time, part-time or as a locum was not associated with patterns of admission, nor was any particular postgraduate certification (e.g., CCFP, CCFP EM, FRCPC) or any of the surveyed attitudinal traits.Conclusion:Individual EPs' overall and CTAS-specific admissions varied substantially, and followed an approximately normal distribution curve. Emergency physicians with more years of experience had a statistically higher CTAS-2 admission rate; however, other variables, including postgraduate certification status, decision-related attitudes toward admission, and reported practices were not associated with admission proportions. Emergency physicians tend to have uniquely individual admission ranking profiles across all the CTAS categories.
Regional self-reliance for livestock feed, meat, dairy and eggs in the Northeast USA
Authors: Conrad Zach , Tichenor Nicole E. , Peters Christian J. , Griffin Timothy S. 
Published: April 2016

AbstractThe production of livestock feed in the USA is geographically concentrated, which poses several risks. Extreme weather events and disease outbreaks have the potential to disrupt production in these areas, which could reduce the national output of meat, dairy and eggs. Additionally, geographically concentrated livestock and feed production systems have been observed to contribute excessive nutrient loads to surrounding soil and water bodies, thereby threatening environmental sustainability. Geographic relocation of production systems has been proposed as an adaptation strategy to increase system resilience and this could take the shape of more geographically dispersed livestock feed production. We estimate the degree to which the demand for meat, dairy and eggs in the Northeast region is met with current levels of regional feed and livestock production, a term that we refer to as regional self-reliance. We combine mean annual (2001–2010) data on Northeast regional land use; crop output; meat, dairy and egg output; and food consumption with a livestock feed requirements model. An annual mean of over 6.1 million ha of land in the Northeast was dedicated to livestock feed from 2001 to 2010, with nearly 80% located in just three states (Pennsylvania, New York and West Virginia). The region is a net importer of livestock feed (in terms of total digestible nutrients and crude protein), as well as meat, dairy and eggs (in terms of total human-edible energy and protein). This is the result of a confluence of long-term regional trends that include the movement of agricultural production out of the region with a concomitant increase in the regional population and an increase in the national demand for meat, dairy and eggs. Limited slaughter output in the region is a key limiting factor to increasing the region's self-reliance for livestock products.
PINK1 and Parkin cooperatively protect neurons against constitutively active TRP channel-induced retinal degeneration in Drosophila
Authors: Huang Z , Ren S , Jiang Y , Wang T 
Published: April 2016

AbstractCalcium has an important role in regulating numerous cellular activities. However, extremely high levels of intracellular calcium can lead to neurotoxicity, a process commonly associated with degenerative diseases. Despite the clear role of calcium cytotoxicity in mediating neuronal cell death in this context, the pathological mechanisms remain controversial. We used a well-established Drosophila model of retinal degeneration, which involves the constitutively active TRPP365 channels, to study calcium-induced neurotoxicity. We found that the disruption of mitochondrial function was associated with the degenerative process. Further, increasing autophagy flux prevented cell death in TrpP365 mutant flies, and this depended on the PINK1/Parkin pathway. In addition, the retinal degeneration process was also suppressed by the coexpression of PINK1 and Parkin. Our results provide genetic evidence that mitochondrial dysfunction has a key role in the pathology of cellular calcium neurotoxicity. In addition, the results demonstrated that maintaining mitochondrial homeostasis via PINK1/Parkin-dependent mitochondrial quality control can potentially alleviate cell death in a wide range of neurodegenerative diseases.
Targeting the epigenetics of the DNA damage response in breast cancer
Authors: Montenegro M F , González-Guerrero R , Sánchez-del-Campo L , Piñero-Madrona A , Cabezas-Herrera J , Rodríguez-López J N 
Published: April 2016

AbstractCancer is as much an epigenetic disease as it is a genetic disease, and epigenetic alterations in cancer often serve as potent surrogates for genetic mutations. Because the epigenetic factors involved in the DNA damage response are regulated by multiple elements, therapies to target specific components of the epigenetic machinery can be inefficient. In contrast, therapies aimed at inhibiting the methionine cycle can indirectly inhibit both DNA and protein methylation, and the wide variety of genes and pathways that are affected by these methylations make this global strategy very attractive. In the present study, we propose an adjuvant therapy that targets the epigenetics of the DNA damage response in breast cancer cells and that results in efficient apoptosis and a reduction in distant metastases in vivo. We observed that a combined therapy designed to uncouple adenosine metabolism using dipyridamole in the presence of a new synthetic antifolate, 3-O-(3,4,5-trimethoxybenzoyl)-(−)-catechin, simultaneously and efficiently blocked both the folic cycle and the methionine cycle in breast cancer cells and sensitized these cells to radiotherapy. The treatment impeded the recruitment of 53BP1 and BRCA1 to the chromatin regions flanking DNA double-strand breaks and thereby avoided the DNA damage responses in breast cancer cells that were exposed to ionizing radiation. In addition, this hypomethylating therapy was also efficient in reducing the self-renewal capability of breast cancer-initiating cells and induced reversion of mesenchymal phenotypes in breast cancer cells.
β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy
Authors: Liu J , Li Q X , Wang X J , Zhang C , Duan Y Q , Wang Z Y , Zhang Y , Yu X , Li N J , Sun J P , Yi F 
Published: April 2016

Abstractβ-Arrestins are multifunctional proteins originally identified as negative adaptors of G protein-coupled receptors (GPCRs). Emerging evidence has also indicated that β-arrestins can activate signaling pathways independent of GPCR activation. This study was to elucidate the role of β-arrestins in diabetic nephropathy (DN) and hypothesized that β-arrestins contribute to diabetic renal injury by mediating podocyte autophagic process. We first found that both β-arrestin-1 and β-arrestin-2 were upregulated in the kidney from streptozotocin-induced diabetic mice, diabetic db/db mice and kidney biopsies from diabetic patients. We further revealed that either β-arrestin-1 or β-arrestin-2 deficiency (Arrb1−/− or Arrb2−/−) ameliorated renal injury in diabetic mice. In vitro, we observed that podocytes increased both β-arrestin-1 and β-arrestin-2 expression levels under hyperglycemia condition and further demonstrated that β-arrestin-1 and β-arrestin-2 shared common mechanisms to suppress podocyte autophagy by negative regulation of ATG12–ATG5 conjugation. Collectively, this study for the first time demonstrates that β-arrestin-1 and β-arrestin-2 mediate podocyte autophagic activity, indicating that β-arrestins are critical components of signal transduction pathways that link renal injury to reduce autophagy in DN. Modulation of these pathways may be an innovative therapeutic strategy for treating patients with DN.