bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation on the interaction of collagen molecules in solution with different acetic acid concentrations
Authors: Yang Huan , Deng Yi , Xu Songcheng , Liu Wentao , Li Guoying 
Published: May 2017

ABSTRACTCollagen solutions in the presence of 0.1–2.0M acetic acid (AA) were investigated to understand the interaction between collagen molecules and an acidic solvent. Fluorescence measurements of pyrene showed that the critical aggregation concentration (CAC) of collagen increased from 0.518 to 1.581 mg/mL for AA concentrations ranging from 0.1 to 2.0M, indicating that the aggregated state of collagen molecules was associated with AA concentration. The size of the collagen aggregates, determined by dynamic light scattering, demonstrated that their disaggregation was enhanced with increasing electrostatic repulsion between the collagen chains. The variations in the intrinsic viscosity and Huggins coefficient depended on the molecular interaction among the collagen molecules. Furthermore, the increased AA concentration has a different influence on the rigidity of collagen molecules above and below the CAC. This observation was manifested by the changes in the morphology of collagen molecules observed by atomic force microscopy. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45255.
Office waste paper as cellulose nanocrystal source
Authors: Orue Ander , Santamaria‐Echart Arantzazu , Eceiza Arantxa , Peña‐Rodriguez Cristina , Arbelaiz Aitor 
Published: May 2017

ABSTRACTCellulose nanocrystals (CNC) are isolated from office waste paper using an alkali solution and a subsequent acid hydrolysis process. The Fourier transform infrared spectroscopy and X‐ray diffraction (XRD) results demonstrate that ink and fillers used in the papermaking industry are almost totally removed after alkali treatments. The XRD results show that CNCs obtained after 2 wt % NaOH solution treatment and a subsequent hydrolysis process exhibit only a cellulose I crystalline structure, and the crystallinity index value increases around 42% with respect to initial office waste paper. Nevertheless, CNCs obtained after 7.5 wt % NaOH solution treatment and a subsequent acid hydrolysis process show a mixture of cellulose I and cellulose II polymorphs. The thermal analysis shows that the CNCs obtained after 7.5 wt % NaOH solution treatment and a subsequent acid hydrolysis process are thermally less stable than other samples, suggesting that the cellulose chains could depolymerize into low molecular weight sugar compounds. Even though the atomic force microscopy images confirm the presence of CNCs, the optical images show that some cellulose microfibers still maintain their structure. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45257.
Effects of poly(propylene carbonate) additive prepared from carbon dioxide on the tensile properties of polypropylene
Authors: Takubo Etsuko , Hiejima Yusuke , Nishioka Kiyoshi , Nitta Koh‐hei 
Published: May 2017

ABSTRACTWe investigated the effect of a rubbery poly(propylene carbonate) (PPC) additive on the tensile properties of isotactic polypropylene (iPP). PPC materials were produced from carbon dioxide gas. Although the elongation of iPP was reduced at higher elongation speeds, PPC addition improved the elongation at break at higher elongation speeds. iPP/PPC blends showed a high drawability while maintaining a high stress level. The failure mechanisms at high elongation speeds were different between the iPP and iPP/PPC samples. Craze‐like voids appeared along the stretching direction for pure iPP, whereas the PPC additive yielded craze‐like voids that were perpendicular to the stretching direction. The addition of PPC doped with carbon nanodots enhanced the yield toughness of iPP at high elongation speeds. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45266.
Dual‐fluorophore Raspberry‐like Nanohybrids for Ratiometric pH Sensing
Authors: Acquah Isaac , Roh Jinkyu , Ahn Dong June 
Published: May 2017

AbstractWe report on the development of raspberry‐like silica structures formed by the adsorption of 8‐hydroxypyrene‐1,3,6‐trisulfonate (HPTS)@silica nanoparticles (NPs) on rhodamine B isothiocyanate (RBTIC)@silica NPs for ratiometric fluorescence‐based pH sensing. To overcome the well‐known problem of dye leaching which occurs during encapsulation of anionic HPTS dye in silica NPs, we utilized a polyelectrolyte‐assisted incorporation of the anionic HPTS. The morphological and optical characterization of the as‐synthesized dye‐doped NPs and the resulting nanohybrids were carried out. The pH‐sensitive dye, HPTS, incorporated in the HPTS‐doped silica NPs provided a pH‐dependent fluorescence response while the RBITC‐doped silica provided the reference signal for ratiometric sensing. We evaluated the effectiveness of the nanohybrids for pH sensing; the ratio of the fluorescence emission intensity at 510 nm and 583 nm at excitation wavelengths of 454 nm and 555 nm, respectively. The results showed a dynamic response in the acidic pH range. With this approach, nanohybrids containing different dyes or receptors could be developed for multifunctioning and multiplexing applications.
Polysome profiling of mAb producing CHO cell lines links translational control of cell proliferation and recombinant mRNA loading onto ribosomes with global and recombinant protein synthesis
Authors: Godfrey Charlotte L. , Mead Emma J. , Daramola Olalekan , Dunn Sarah , Hatton Diane , Field Ray , Pettman Gary , Smales C. Mark 
Published: May 2017

AbstractmRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to reprogramming of translation and decreased global protein synthesis. Here we apply polysome profiling to determine reprogramming and translational capabilities in host and recombinant monoclonal antibody‐producing (mAb) CHO cell lines during batch culture. Recombinant cell lines with the fastest cell specific growth rates were those with the highest global translational efficiency. However, total ribosomal capacity, determined from polysome profiles, did not relate to the fastest growing or highest producing mAb cell line, suggesting it is the ability to utilise available machinery that determines protein synthetic capacity. Cell lines with higher cell specific productivities tended to have elevated recombinant heavy chain transcript copy numbers, localised to the translationally active heavy polysomes. The highest titre cell line was that which sustained recombinant protein synthesis and maintained high recombinant transcript copy numbers in polysomes. Investigation of specific endogenous transcripts revealed a number that maintained or reprogrammed into heavy polysomes, identifying targets for potential cell engineering or those with 5' untranslated regions that might be utilised to enhance recombinant transcript translation.
Epigenetic silencing of ADAMTS18 promotes cell migration and invasion of breast cancer through AKT and NF‐κB signaling
Authors: Xu Hongying , Xiao Qian , Fan Yu , Xiang Tingxiu , Li Chen , Li Chunhong , Li Shuman , Hui Tianli , Zhang Lu , Li Hongzhong , Li Lili , Ren Guosheng 
Published: May 2017

AbstractADAMTS18 dysregulation plays an important role in many disease processes including cancer. We previously found ADAMTS18 as frequently methylated tumor suppressor gene (TSG) for multiple carcinomas, however, its biological functions and underlying molecular mechanisms in breast carcinogenesis remain unknown. Here, we found that ADAMTS18 was silenced or downregulated in breast cancer cell lines. ADAMTS18 was reduced in primary breast tumor tissues as compared with their adjacent noncancer tissues. ADAMTS18 promoter methylation was detected in 70.8% of tumor tissues by methylation‐specific PCR, but none of the normal tissues. Demethylation treatment restored ADAMTS18 expression in silenced breast cell lines. Ectopic expression of ADAMTS18 in breast tumor cells resulted in inhibition of cell migration and invasion. Nude mouse model further confirmed that ADAMTS18 suppressed breast cancer metastasis in vivo. Further mechanistic studies showed that ADAMTS18 suppressed epithelial‐mesenchymal transition (EMT), further inhibited migration and invasion of breast cancer cells. ADAMT18 deregulated AKT and NF‐κB signaling, through inhibiting phosphorylation levels of AKT and p65. Thus, ADAMTS18 as an antimetastatic tumor suppressor antagonizes AKT and NF‐κB signaling in breast tumorigenesis. Its methylation could be a potential tumor biomarker for breast cancer.
Chemical Composition, Antibacterial, Schistosomicidal, and Cytotoxic Activities of the Essential Oil of Dysphania ambrosioides (L.) Mosyakin & Clemants (Chenopodiaceae)
Authors: Soares Marina H. , Dias Herbert J. , Vieira Tatiana M. , de Souza Maria G. M. , Cruz Ana F. F. , Badoco Fernanda R. , Nicolella Heloiza D. , Cunha Wilson R. , Groppo Milton , Martins Carlos H. G. , Tavares Denise C. , Magalhães Lizandra G. , Crotti Antônio E. M. 
Published: May 2017

We have investigated the chemical composition and the antibacterial activity of the essential oil of Dysphania ambrosioides (L.) Mosyakin & Clemants (Chenopodiaceae) (DA‐EO) against a representative panel of cariogenic bacteria. We have also assessed the in vitro schistosomicidal effects of DA‐EO on Schistosoma mansoni and its cytotoxicity to GM07492‐A cells in vitro. Gas chromatography (GC) and gas chromatography‐mass spectrometry (GC/MS) revealed that the monoterpenes cis‐piperitone oxide (35.2%), p‐cymene (14.5%), isoascaridole (14.1%), and α‐terpinene (11.6%) were identified by as the major constituents of DA‐EO. DA‐EO displayed weak activity against Streptococcus sobrinus and Enterococcus faecalis (minimum inhibitory concentration (MIC) = 1000 μg/ml). On the other hand, DA‐EO at 25 and 12.5 μg/ml presented remarkable schistosomicidal action in vitro and killed 100% of adult worm pairs within 24 and 72 h, respectively. The LC50 values of DA‐EO were 6.50 ± 0.38, 3.66 ± 1.06, and 3.65 ± 0.76 μg/ml at 24, 48, and 72 h, respectively. However, DA‐EO at concentrations higher than 312.5 μg/ml significantly reduced the viability of GM07492‐A cells (IC50 = 207.1 ± 4.4 μg/ml). The selectivity index showed that DA‐EO was 31.8 times more toxic to the adult S. mansoni worms than GM07492‐A cells. Taken together, these results demonstrate the promising schistosomicidal potential of the essential oil of Dysphania ambrosioides.
Polyadenine‐Modulated DNA Conformation Monitored by Surface‐Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application
Authors: Guo Jingxing , Chen Yunlong , Jiang Yongjia , Ju Huangxian 
Published: May 2017

AbstractThis work proposes a facile way to modulate the conformation of DNA from the “Lie‐Down” to the “Stand‐Up” conformation on the surface of multibranched gold nanoparticles (AuNPs). This is realized by regulating the length of polyadenine (polyA) linked to the DNA sequence and/or the hybridization of this sequence with the target DNA, and can be monitored by the Raman signal owing to the excellent performance of multibranched AuNPs (AuNSs) as a surface‐enhanced Raman scattering (SERS) substrate and the distance change between the Raman reporter and the substrate. The probable mechanism, which depends on the repulsion of polyA from the sequence and the tip assembly, has also been probed through theoretical simulation using the finite difference time domain method. By virtue of this strategy, a conformation‐transformation‐based DNA@AuNS sensor is constructed for the identification of a specific oligonucleotide, which has been used for the detection of DNA sequences associated with Severe Acute Respiratory Syndrome (SARS). This strategy leads to a novel sensing platform with good extendibility for DNA analysis, and provides a powerful protocol for facilitating the cognition of DNA conformation on metal surfaces.
Observation of Supramolecular Chirality in a Hierarchically Self‐Assembled Mixed‐Stack Charge‐Transfer Complex
Authors: Krishnan Sumesh Babu , Gopidas Karical Raman 
Published: May 2017

AbstractSupramolecular chirality was observed in a charge‐transfer (CT) complex formed by self‐assembly of β‐cyclodextrin‐linked pyrene and adamantane‐linked pyromellitic diimide in water. In the complex, the pyrene–pyromellitic diimide CT interaction is reinforced several‐fold by the strong β‐cyclodextrin–adamantane inclusion binding interaction, as confirmed by 1H NMR, absorption, fluorescence and isothermal titration calorimetry studies. The CT complex exhibited high stability, as inferred from the temperature‐dependent absorption and NMR studies. The CT absorption band exhibited a positive Cotton effect indicative of supramolecular chirality for the complex. Twisted fibres were observed in TEM, AFM, and SEM experiments. A mechanism involving synergistic binding interactions leading to 2D sheets, followed by twisting of the sheet, is proposed for the formation of twisted fibres. This system offers a chance to investigate a combination of supramolecular CT and supramolecular chirality and explore their synergistic effects.
Ruthenium Complexes with Strongly Electron‐Donating Terpyridine Ligands: Effect of the Working Electrode on Electrochemical and Spectroelectrochemical Properties
Authors: Klein Johannes , Stuckmann Alexandra , Sobottka Sebastian , Suntrup Lisa , van der Meer Margarethe , Hommes Paul , Reissig Hans‐Ulrich , Sarkar Biprajit 
Published: May 2017

AbstractThe combination of 2,2′:6′,2′′‐terpyridines (tpy) and RuII is known to deliver molecular and supramolecular assemblies with remarkable properties. Here new RuII complexes, with modified tpy ligands substituted with varying numbers of dimethlyamino groups, are presented. Electrochemistry shows that the incorporation of the strongly electron‐donating groups on the tpy ligands leads to a negative shift of the RuII oxidation potential by close to 1 V. The reductive electrochemical responses are strongly dependent on the nature of the working electrode, with glassy carbon and gold working electrodes showing the best results. These observations led to the development of a modified Optically Transparent Thin Layer Electrochemical (OTTLE) cell, based on a gold working electrode. The use of UV/Vis/NIR spectroelectrochemical methods with that OTTLE cell, together with simulations of the cyclic voltammograms, allowed the characterization of four reduction steps in these complexes, the final two of which lead to bond activations at the ruthenium center. This observation is to the best of our knowledge unprecedented in coordinatively saturated complexes of type [Ru(tpy)2]2+. The various redox states of the complexes were characterized by EPR spectroelectrochemistry and through DFT calculations. The results presented here establish these substituted tpy ligands as highly attractive ligands in coordination chemistry, and display the utility of a gold‐based OTTLE cell for spectroelectrochemical measurements.
Equilibrium, Kinetic and Structural Properties of Gallium(III) and Some Divalent Metal Complexes Formed with the New DATAm and DATA5m Ligands
Authors: Farkas Edit , Nagel Johannes , Waldron Bradley P. , Parker David , Tóth Imre , Brücher Ernő , Rösch Frank , Baranyai Zsolt 
Published: May 2017

AbstractThe development of 68Ge/68Ga generators has made the positron‐emitting 68Ga isotope widely accessible and raised interest in new chelate complexes of Ga3+. The hexadentate 1,4‐di(acetate)‐6‐methyl[amino(methyl)acetate]perhydro‐1,4‐diazepane (DATAm) ligand and its bifunctional analogue, 1,4‐di(acetate)‐6‐pentanoic acid[amino(methyl)acetate]perhydro‐1,4‐diazepane (DATA5m), rapidly form complexes with 68Ga in high radiochemical yield. The stability constants of DATAm and DATA5m complexes formed with Ga3+, Zn2+, Cu2+, Mn2+ and Ca2+ have been determined by using pH potentiometry, spectrophotometry (Cu2+) and 1H and 71Ga NMR spectroscopy (Ga3+). The stability constants of Ga(DATAm) and Ga(DATA5m) complexes are slightly higher than those of Ga(AAZTA). The species distribution calculations indicated the predominance of Ga(L)OH mixed‐hydroxo complexes at physiological pH. The 1H and 71Ga NMR spectroscopy studies provided information about the coordinated functional groups of ligands and on the kinetics of exchange between the Ga(L) and Ga(L)OH complexes. The transmetalation reactions between the Ga(L) complexes and Cu2+ citrate (6<pH<8.5) occur through both spontaneous and OH−‐assisted dissociation of the Ga(L)OH species. At pH 7.4 and 25 °C, the half‐lives of the dissociation of Ga(DATAm), Ga(DATA5m) and Ga(AAZTA) were 11, 44 and 24 h, respectively. Similar half‐lives have been obtained for the ligand‐exchange reactions between the Ga(L)OH complexes and transferrin. The equilibrium and kinetic data indicate that the Ga(DATA5m) complex is a good 68Ga‐based radiodiagnostic candidate.
Mechanochemical Synthesis of Phosphazane‐Based Frameworks
Authors: Sim Ying , Shi Yan X. , Ganguly Rakesh , Li Yongxin , García Felipe 
Published: May 2017

AbstractMechanochemistry is emerging as a powerful solvent‐free approach to chemical synthesis, having been applied to metal oxides, pharmaceutical materials, organic compounds and to a lesser extent, coordination complex synthesis. Notably, examples of applications of mechanochemical methodologies in the synthesis of main‐group compounds are few and far between. Herein, we demonstrate that ball milling enabled the solvent‐free synthesis of a range of phosphazane frameworks with a broad substrate scope, yielding seven new acyclic and macrocyclic species. The strength of this methodology is highlighted by a fast, selective and high‐conversion product generation from poorly soluble starting materials, thereby demonstrating mechanochemistry as a real alternative to solution‐based methods in synthetic main‐group chemistry.
Inkjet Printing of Aqueous Photoluminescent CdSe/CdS Nanorods on Solid Substrates
Authors: Lübkemann Franziska , Anselmann Ralf , Kodanek Torben , Bigall Nadja‐Carola 
Published: May 2017

AbstractIn order to deposit colloidal nanocrystals on different substrates, a large variety of methodologies have been developed, such as dipcoating, spin coating, or spray coating. The deposition of CdSe/CdS core‐shell nanorods (NRs) with a conventional office printer onto conducting tin‐doped indium oxide glass surfaces is presented. This involves the preparation of water‐based inks containing the NRs. To improve the connection between the CdSe/CdS NRs and the underlying substrates, the glass surfaces were modified by means of silanes. Homogeneous films were obtained by consecutively printing several layers of CdSe/CdS NRs.
The Density Distribution in Ribbons from Roll Compaction
Authors: Wiedey Raphael , Kleinebudde Peter 
Published: May 2017

AbstractThe most important quality attribute in roll compaction/dry granulation is the ribbon porosity, as it predominantly determines the granule porosity and granule size distribution. The porosity, however, is not distributed homogeneously over the ribbon. In this study the porosity distribution was investigated using X‐ray micro‐computed tomography and demonstrated that characteristic distribution patterns exist over all three spatial directions, the ribbon length, width, and thickness. In detail the distribution patterns depend on the sealing system.
Development of Element Technology for 1 STEP Biomarker Protein Analysis Device Using Silver Nanoparticle–Contained Hydrogel and Reagent‐Immobilized Cartridge
Authors: YAMAMOTO KENZO , SUEYOSHI KENJI , HISAMOTO HIDEAKI , ENDO TATSURO 
Published: May 2017

SUMMARYWe fabricated 1 STEP biomarker protein analysis device using silver nanoparticles (AgNPs)‐contained hydrogel and reagent‐immobilized cartridge. Specific color change of AgNPs based on localized surface plasmon resonance (LSPR) was applied to develop the novel colorimetric protein analysis device. In this report, we constructed this device by four components, AgNPs‐contained hydrogel, glucose oxidase (GOx) antibody release pad, glucose release pad, and antibody‐immobilized membrane. AgNPs‐contained hydrogel detected 0.001 to 10 μg/mL GOx‐antibody. AgNPs‐contained hydrogel absorbance became altered 90.0% GOx release pad as compared to GOx‐antibody solution under the reaction time 60 min. AgNPs‐contained hydrogel absorbance became altered 98.4% glucose release pad as compared to glucose solution under the reaction time 60 min. We applied AgNPs‐contained hydrogel and antibody‐immobilized membrane to detect human immunoglobulin G (IgG). As a result, we found these could detect 1 μg/mL or lower concentration of human IgG.
Potential impacts of overlapping land‐use and climate in a sensitive dryland: a case study of the Colorado Plateau, USA
Authors: Copeland Stella M. , Bradford John B. , Duniway Michael C. , Schuster Rudy M. 
Published: May 2017

AbstractThe combination of co‐occurring climate change and increasing land‐use is likely to affect future environmental and socioeconomic conditions in drylands; these hyper‐arid to sub‐humid landscapes are limited by water resources and prone to land degradation. We characterized the potential for geographic overlap among land‐use practices and between land‐use and climate change on the Colorado Plateau—a dryland region experiencing rapid changes in land‐use and facing aridification. We characterized spatial patterns and temporal trends in aridification, land‐use, and recreation at the county and 10‐km2 grid scales. Increasing trends and overlapping areas of high intensity for use, including oil and gas development and recreation, and climate drying, suggest areas with high potential to experience detrimental effects to the recreation economy, water availability, vegetation and wildlife habitat, and spiritual and cultural resources. Patterns of overlap in high‐intensity land‐use and climate drying differ from the past, indicating the potential for novel impacts and suggesting that land managers and planners may require new strategies to adapt to changing conditions. This analytical framework for assessing the potential impacts of overlapping land‐use and climate change could be applied with other drivers of change or to other regions to create scenarios at various spatial scales in support of natural resource planning efforts.