bluuebunny/crossref_metadata_2025_split huggingface dataset:
ПСИХІЧНІ СТАНИ В СТРУКТУРІ ПЕРЕЖИВАННЯ ПРОФЕСІЙНОЇ КРИЗИ
Authors: Вербицька Л. 
Published: October 2024

У статті розкрито теоретико-методологічні підходи різних авторів щодо розкриття сутнісних ознак поняття «професійна криза» та «психічних станів». Проаналізовано наслідки феномену професійної кризи та можливий негативний вплив на ефективність життєдіяльності особистості. Розглянуто психологічні особливості професійної кризи та негативні психічні стани. Розкрито фактори, що детермінують виникнення та перебіг криз професійного розвитку людини. За підсумками концепції професійного становлення особистості виділяються стадії професійних криз та можливі негативні наслідки перебігу кризових етапів та пропонуються конструктивні варіанти їх подолання. Розглянуто механізми формування та прояву криз професійного становлення особистості, особливості їх розвитку та перебігу.
First Two Cases of Russell’s Viper (Daboia russelii)bite, aRe-emerged Cause of Envenomation in Bangladesh
Authors: Amin Md Robed , Rahman Abu Shahin Mohammed Mahbubur , Haque Md Azizul , Haque Md Zahirul , Rahman F M Atiqur , Chowdhury Fazle Rabbi , Kuch Ulrich , Faiz Md Abul 
Published: October 2024

The first two cases of Russell’s viper bite (RV)presented in contemporary period from Chapainwabgonj and Patuakhali districts of Bangladesh in 2013. The unfortunate death of both the cases indicates the severity it can produce and difficulties in its management in resource limited settings. Treatment seeking from traditional healer, sub-district hospital could not provide first dose of AV before referral and delayed arrival to hospital are concerned to be addressed to prevent death from bite by RV in future. Basic preventive measures such as careful while working and walking in the field and unnecessary handling of snake could avoid bite in both the cases.
Bangladesh. Bangladesh J Medicine 2024; 35: 204-210
ЖИТТЄПИС ОСТАННЬОГО ПРОВІДНИКА ЛУЦЬКОЇ ОКРУГИ ОУН ОЛЕКСАНДРА САВИРИ («ЯРОША»)
Authors: Антонюк Ярослав Миколайович , Трофимович Володимир Васильович 
Published: October 2024

У статті проаналізовано життєвий шлях одного з видатних лідерів визвольного руху 1940-х – 1950-х років на теренах Волині – Олександра Савири. З’ясовано склад його родини та досліджено юнацький період життя. Доведено, що Олександр Савира діяв під псевдонімами «Адам», «Антон», «Аркадій», «Арсен», «Блакит», «Вадим», «Зарічний», «Зозуля», «Івась», «Лаврін», «Оксентій», «Роман», «Савка», «Назар», а найчастіше – «Ярош». Його робота як керівника розпочалась у Службі безпеки. Спочатку на теренах Сарненської, а згодом – Холмської округи ОУН. Після проходження фронту «Ярош» повернувся на Волинь та очолив Володимирський надрайон ОУН. Влітку 1945 р. його підвищили до посади керівника пропаганди Північно-Західного краю (ПЗК) «Москва» та водночас терену «Крим» (Луцький і Торчинський райони), де базувалося крайове керівництво. На початку 1949 р. він отримав посаду керівника СБ Луцької округи ОУН, а в жовтні 1950 р. очолив провід цього терену. З того часу його постать стала ключовою у діяльності підпілля ОУН південних районів Волині. Особливо помітно вплив «Яроша» зріс на початку 1950-х років. У цей період він забезпечував роботу лінії зв’язку між керівництвом Проводу ОУН у Галичині та підпіллям ОУН Волині й Західного Полісся. Неодноразово «Ярош» особисто зустрічався з провідником ОУН на Північно-західних українських землях (ПЗУЗ) ОУН Василем Галасою («Орланом») і головнокомандувачем Української повстанської армії (УПА) Василем Куком («Лемішем»). Активна діяльність провідника Луцької округи ОУН приковувала до нього увагу співробітників радянських спецслужб – Міністерства державної безпеки (МДБ) і Міністерства внутрішніх справ (МВС). Використовуючи розгалужену агентурну мережу, 14 травня 1953 р. вони виявили та ліквідували «Яроша».
Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal
Authors: Fazel Anvari Yazdi Abbas , Tahermanesh Kobra , Ejlali Maryam , Babaei-Ghazvini Amin , Acharya Bishnu , Badea Ildiko , MacPhee Daniel J. , Chen Xiongbiao 
Published: October 2024

IntroductionDecellularized uterine extracellular matrix has emerged as a pivotal focus in the realm of biomaterials, offering a promising source in uterine tissue regeneration, research on disease diagnosis and treatments, and ultimately uterine transplantation. In this study, we examined various protocols for decellularizing porcine uterine tissues, aimed to unravel the intricate dynamics of DNA removal, bioactive molecules preservation, and microstructural alterations.MethodsPorcine uterine tissues were treated with 6 different, yet rigorously selected and designed, protocols with sodium dodecyl sulfate (SDS), Triton® X-100, peracetic acid + ethanol, and DNase I. After decellularization, we examined DNA quantification, histological staining (H&amp;E and DAPI), glycosaminoglycans (GAG) assay, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and Thermogravimetric Analysis (TGA).ResultsA comparative analysis among all 6 protocols was conducted with the results demonstrating that all protocols achieved decellularization; while 0.1% SDS + 1% Triton® X-100, coupled with agitation, demonstrated the highest efficiency in DNA removal. Also, it was found that DNase I played a key role in enhancing the efficiency of the decellularization process by underscoring its significance in digesting cellular contents and eliminating cell debris by 99.79% (19.63 ± 3.92 ng/mg dry weight).ConclusionsOur findings enhance the nuanced understanding of DNA removal, GAG preservation, microstructural alteration, and protein decomposition in decellularized uterine extracellular matrix, while highlighting the importance of decellularization protocols designed for intended applications. This study along with our findings represents meaningful progress for advancing the field of uterine transplantation and related tissue engineering/regenerative medicine.
The influence of adult urine lead exposure on bone mineral densit: NHANES 2015-2018
Authors: Wang Shaokang , Zhao Xiaofeng , Zhou Runtian , Jin Yuanzhang , Wang Xiaonan , Ma Xiaotian , Lu Xiangdong 
Published: October 2024

IntroductionPrevious studies have indicated that exposure to heavy metals related to bone health is primarily limited to some common harmful metals, and the impact of lead has not been fully understood. This study aims to explore the relationship between urine lead exposure and bone density.Methods1,310 adults were included from the NHANES database (2015-2018), and through generalized linear regression analysis and constrained cubic spline models, the association between lead levels and total bone density as well as lumbar spine bone density was explored. The study also examined the impact of combined exposure to lead and cadmium on bone density.Results and conclusionsUrinary lead levels were significantly negatively correlated with total bone mineral density (β: −0.015; 95%CI: −0.024, −0.007) and lumbar spine bone mineral density (β: −0.019; 95%CI: −0.031, −0.006). Compared to the lowest three quartiles of lead levels, the adjusted odds ratios for T3 changes in total bone mineral density and lumbar spine bone mineral density were 0.974 (95%CI: 0.959, 0.990) and 0.967 (95%CI: 0.943, 0.991), indicating a significant negative trend. Further analysis with constrained cubic spline models revealed a non-linear decreasing relationship between urinary lead and total bone mineral density as well as lumbar spine bone mineral density. Stratified analyses suggested that the relationship between urinary lead levels and bone mineral density might be significantly influenced by age, while gender showed no significant impact on the relationship. Moreover, combined exposure to lead and cadmium was found to be associated with decreased bone mineral density, emphasizing the potential synergistic effects between lead and cadmium on bone health. However, the specific mechanisms of lead and its effects on different populations require further comprehensive research. This study provides valuable insights for further exploration and development of relevant public health policies.
Modulating effects of temperature on CO2-inhibited isoprene emissions in Eucalyptus urophylla
Authors: Pang Weihua , Zhang Yanli , Zeng Jianqiang , Tian Xiao , Wang Xinming 
Published: October 2024

Terrestrial vegetation emits substantial amounts of highly reactive isoprene, significantly impacting atmospheric chemistry and climate change. Both atmospheric carbon dioxide (CO2) concentration and temperature can influence plant isoprene emissions; however, whether these factors have a synergistic effect remains unclear, particularly for tropical/subtropical plants. In this study, we conducted in-situ controlled experiments on Eucalyptus urophylla, a representative tropical/subtropical species, to investigate the seasonal variation in the response of isoprene emissions to CO2 concentrations (ISOP-CO2 response) and to identify potential controlling factors. The results showed that high CO2 exerts a nearly linear inhibitory effect on isoprene emissions, as indicated by the slope of the ISOP-CO2 response curve. This inhibitory effect exhibited evident seasonal changes, with stronger suppression during cooler seasons and weaker suppression during warmer seasons. This finding contrasts with the default ISOP-CO2 response in the MEGAN model, which ignored seasonal variation. Further analysis showed a significant correlation between the slope of the ISOP-CO2 response curve and growth temperature from the past 10 days, indicating that these metrics are effective indicators for predicting seasonal changes. Our findings reveal a synergistic mechanism between temperature and CO2 concentration effects on isoprene emissions. By coupling the effects of growth temperature with the ISOP-CO2 response, this mechanism can be integrated into models to provide more accurate predictions of future isoprene emissions, reducing prediction biases, especially during cooler seasons.
Molecular diagnosis of patients with syndromic short stature identified by trio whole-exome sequencing
Authors: Sun Huihui , Zhang Geng , Li Na , Bu Xiangfang 
Published: October 2024

BackgroundShort stature is a complex disorder with phenotypic and genetic heterogeneity. This study aimed to investigate clinical phenotypes and molecular basis of a cohort of patients with short stature.MethodsTrio whole-exome sequencing (Trio-WES) was performed to explore the genetic aetiology and obtain a molecular diagnosis in twenty Chinese probands with syndromic and isolated short stature.ResultsOf the twenty probands, six (6/20, 30%) patients with syndromic short stature obtained a molecular diagnosis. One novel COMP pathogenic variant c.1359delC, p.N453fs*62 and one LZTR1 likely pathogenic variant c.509G&gt;A, p.R170Q were identified in a patient with short stature and skeletal dysplasia. One novel de novo NAA15 pathogenic variant c.63T&gt;G, p.Y21X and one novel de novo KMT2A pathogenic variant c.3516T&gt;A, p.N1172K was identified in two probands with short stature, intellectual disability and abnormal behaviours, respectively. One patient with short stature, cataract, and muscle weakness had a de novo POLG pathogenic variant c.2863 T&gt;C, p.Y955H. One PHEX pathogenic variant c.1104G&gt;A, p.W368X was identified in a patient with short stature and rickets. Maternal uniparental disomy 7 (mUPD7) was pathogenic in a patient with pre and postnatal growth retardation, wide forehead, triangular face, micrognathia and clinodactyly. Thirteen patients with isolated short stature had negative results.ConclusionTrio-WES is an important strategy for identifying genetic variants and UPD in patients with syndromic short stature, in which dual genetic variants are existent in some individuals. It is important to differentiate between syndromic and isolated short stature. Genetic testing has a high yield for syndromic patients but low for isolated patients.
Spermidine attenuates chondrocyte inflammation and cellular pyroptosis through the AhR/NF-κB axis and the NLRP3/caspase-1/GSDMD pathway
Authors: Guo Xiaocheng , Feng Xinyuan , Yang Yue , Zhang He , Bai Lunhao 
Published: October 2024

IntroductionOsteoarthritis (OA) is a prevalent chronic degenerative disease, marked by a complex interplay of mechanical stress, inflammation, and metabolic imbalances. Recent studies have highlighted the potential of spermidine (SPD), a naturally occurring polyamine known for its anti-inflammatory and antioxidant properties, as a promising therapeutic agent for OA. This study delves into the therapeutic efficacy and mechanistic pathways of SPD in mitigating OA symptoms.MethodsForty Sprague-Dawley rats were randomly assigned to four groups, including the CG (sham operation), model (anterior cruciate ligament transection [ACLT], and treatment (ACLT + two different doses of SPD) groups. In vivo, correlations between OA severity and different interventions were assessed by ELISA, X-rays, CT imaging, histological staining, and immunohistochemistry. In vitro, IL-1β was used to trigger chondrocyte inflammation, and SPD’s cytotoxicity was assessed in primary rat chondrocytes. Next, inflammatory markers, extracellular matrix (ECM) proteins, and pathway marker proteins were detected in chondrocytes administered IL-1β alone, SPD, or aryl hydrocarbon receptor (AhR) silencing, by qRT-PCR, Griess reaction, ELISA, Western blot, and immunofluorescence. Morphological alterations and pyroptosis in chondrocytes were examined by transmission electron microscopy (TEM) and flow cytometry.ResultsOur research reveals that SPD exerts significant anti-inflammatory and antipyroptotic effects on IL-1β-treated chondrocytes and in anterior cruciate ligament transection (ACLT) rat models of OA, primarily through interaction with the Aryl hydrocarbon receptor (AhR). Specifically, SPD’s binding to AhR plays a crucial role in modulating the inflammatory response and cellular pyroptosis by inhibiting both the AhR/NF-κB and NLRP3/caspase-1/GSDMD signaling pathways. Furthermore, the knockdown of AhR was found to negate the beneficial effects of SPD, underscoring the centrality of the AhR pathway in SPD’s action mechanism. Additionally, SPD was observed to promote the preservation of cartilage integrity and suppress ECM degradation, further supporting its potential as an effective intervention for OA.DiscussionCollectively, our findings propose SPD as a novel therapeutic approach for OA treatment, targeting the AhR pathway to counteract the disease’s progression and highlighting the need for further clinical evaluation to fully establish its therapeutic utility.
How the arts heal: a review of the neural mechanisms behind the therapeutic effects of creative arts on mental and physical health
Authors: Barnett Kelly Sarah , Vasiu Fabian 
Published: October 2024

BackgroundThe creative arts have long been known for their therapeutic potential. These modalities, which include dance, painting, and music, among others, appear to be effective in enhancing emotional expression and alleviating adverse physiological and psychological effects. Engagement in creative arts can be pursued as a personal hobby, in a classroom setting, or through a formal therapeutic intervention with a qualified therapist. Engagement can be active (i.e., creating) or passive (i.e., viewing, listening). Regardless of the modality and manner of engagement, the mechanisms explaining the therapeutic efficacy of creative arts remain poorly understood.ObjectiveThis study aims to systematically review research investigating the neurological mechanisms activated during active or passive engagement in creative arts, with a specific emphasis on the roles of the medial prefrontal cortex (mPFC) and the amygdala in emotional regulation (ER) and creative behaviors. The review seeks to provide preliminary evidence for the possible existence of common neural mechanisms underlying both phenomena, which could inform the development of targeted therapeutic interventions leveraging creative arts for ER.MethodsA systematic review was conducted following the Cochrane Collaboration guideline and PRISMA standards to identify studies examining the neurological mechanisms underlying creative activities.ResultsA total of six out of 85 records meet the inclusion criteria, with all being basic research studies. Preliminary findings suggest that active and passive engagement with creative arts consistently activate neural circuits implicated in adaptive emotional regulation, including the mPFC and amygdala. These activations mirror the neural pathways engaged in effective ER strategies, suggesting the possible existence of shared mechanisms between creative expression and emotional processing.ConclusionThe evidence underscores the potential of creative arts as a complementary therapeutic strategy alongside conventional care and other evidence-based mind-body modalities. By elucidating the shared neural mechanisms between creative arts engagement and ER, this review contributes to the theoretical and practical understanding of the role of creative arts in mental health. Future research is recommended to further explore these neural correlations and their implications for therapeutic practice.
Case report: Neuroacanthocytosis associated with novel variants in the VPS13A gene with concomitant nucleotide expansion for CANVAS and assessment with osmotic gradient ektacytometry
Authors: Paucar Martin , Wincent Josephine , Rubin Charlotta , Peikert Kevin , Kyhle Josefin , Hertegård Stellan , Möller Riita , Beshara Soheir , Svenningsson Per 
Published: October 2024

Background and objectivesThe diseases historically known as neuroacanthocytosis (NA) conditions include VPS13A disease (formerly chorea-acanthocytosis) and XK disease (formerly McLeod syndrome). Here we report a patient with a hyperkinetic syndrome associated with variants in VPS13A with a concomitant homozygous nucleotide expansion in Replication factor C, subunit 1 (RFC1) and evaluate the role of ektacytometry for the assessment of acanthocytes.MethodsInvestigations included clinical assessments, neuroimaging studies, laboratory analyses, blood smears, ektacytometry, psychometric evaluation, and genetic analyses. Using ektacytometry, an osmoscan curve is obtained yielding a diffraction pattern as a measure of average erythrocyte deformability from circular at rest to elliptical at a high shear stress. The pattern allows the derivation of several parameters (mainly EI-max, O-min and O-Hyper points). Samples from two other patients with genetically proven VPS13A disorder and XK disease and varying numbers of acanthocytes as well as from a fourth with acanthocytosis due to liver failure were also analyzed.Case presentationThe patient has impulsivity, chorea and disabling feeding dystonia refractory to treatment and 15% acanthocytes in peripheral blood. Genetic workup revealed compound heterozygous variants c.1732_1733del; p.(V578Ffs*9) and c.8282C &gt; A, p.(S2761*) in VPS13A with absence of chorein in the blood, the latter variant is novel. In addition, he harbors a homozygous nucleotide expansion in the RFC1 gene, reported in cerebellar ataxia, neuropathy, vestibular areflexia syndrome (CANVAS). However, the patient does not display ataxia yet. Ektacytometry revealed significantly reduced erythrocyte deformability in this patient and in another man with VPS13A disease. In contrast, the patient with XK disease had 2% acanthocytes and mild abnormalities on ektacytometry. In the three cases, ektacytometry yielded a specific pattern, different from acanthocytosis due to liver failure.ConclusionPathogenicity of the VPS13A variants is confirmed by absence of chorein, long-term follow up is required to evaluate any synergistic impact of for the underlying CANVAS mutation. New generation ektacytometry provides an objective measurement of erythrocytes’ rheological properties and may serve as a complement to blood smears. Finally, ektacytometry’s ability to detect deformability of erythrocytes in NA seems to depend on the degree of acanthocytosis.
Molecular determinants of resurgent sodium currents mediated by Navβ4 peptide and A-type FHFs
Authors: Xiao Yucheng , Pan Yanling , Xiao Jingyu , Cummins Theodore R. 
Published: October 2024

IntroductionResurgent current (INaR) generated by voltage-gated sodium channels (VGSCs) plays an essential role in maintaining high-frequency firing of many neurons and contributes to disease pathophysiology such as epilepsy and painful disorders. Targeting INaR may present a highly promising strategy in the treatment of these diseases. Navβ4 and A-type fibroblast growth factor homologous factors (FHFs) have been identified as two classes of important INaR mediators; however, their receptor sites in VGSCs remain unknown, which hinders the development of novel agents to effectively target INaR.MethodsNavβ4 and FHF4A can mediate INaR generation through the amino acid segment located in their C-terminus and N-terminus, respectively. We mainly employed site-directed mutagenesis, chimera construction and whole-cell patch-clamp recording to explore the receptor sites of Navβ4 peptide and FHF4A in Nav1.7 and Nav1.8.ResultsWe show that the receptor of Navβ4-peptide involves four residues, N395, N945, F1737 and Y1744, in Nav1.7 DI-S6, DII-S6, and DIV-S6. We show that A-type FHFs generating INaR depends on the segment located at the very beginning, not at the distal end, of the FHF4 N-terminus domain. We show that the receptor site of A-type FHFs also resides in VGSC inner pore region. We further show that an asparagine at DIIS6, N891 in Nav1.8, is a major determinant of INaR generated by A-type FHFs in VGSCs.DiscussionCryo-EM structures reveal that the side chains of the critical residues project into the VGSC channel pore. Our findings provide additional evidence that Navβ4 peptide and A-type FHFs function as open-channel pore blockers and highlight channel inner pore region as a hotspot for development of novel agents targeting INaR.