bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic Mechanisms of Plant Phosphorus (P) Resorption and Microbial P‐Limitation Affecting Soil P During Grassland Vegetation Succession
Authors: Liu Chunhui , Xue Zhijing , Wang Baorong , Yuan Runtong , Qu Tingting , Zhu Zhaolong , Zhou Zhengchao , An Shaoshan 
Published: September 2024

AbstractPhosphorus (P), a crucial element for all life forms on Earth, is often insufficiently available in terrestrial ecosystems. The interaction and feedback between plants and soil microorganisms are crucial links integrating above‐ and below‐ground ecosystems. However, changes in soil P fractions in response to plant‐microbe interactions during vegetation succession remain poorly understood. This study investigated the trends and relationships between plant resorption, microbial properties, and soil P fractions in the transformation interface soil layer (TIS) and underlying topsoil layer (UTS) during grassland vegetation succession. The results point to a combination of soil properties, alongside microbial and plant factors driving soil P changes. However, the TIS and UTS layers differ, with phosphorus dynamics in the TIS layer primarily influenced by microorganisms. Microorganisms are co‐limited by C and P in both the TIS and UTS layers. Under microbial P‐limitation, microorganisms produce alkaline phosphatases (AP), while C deficiency stimulates the production of C‐acquiring enzymes, subtly regulating soil P dynamics through organic matter decomposition. Plant P resorption efficiency and microbial P‐limitation exhibit synergistic variations, reaching their lowest levels during the mixed Bothriochloa ischaemum and Stipa bungeana Trin (Bo.I + St.B) stage. This study emphasizes that P cycling is influenced by plant‐microbe‐soil interactions and feedback. Plants and soil microorganisms jointly regulate soil nutrient effectiveness and partitioning in the ecosystem.
Miocene Alkaline Basaltic Magmatism in Northeastern Tibetan Plateau: Implications for Mantle Evolution and Plateau Outward Growth
Authors: Che Yue , Liu Dong , Zhao Zhidan , Niu Yaoling , Teng Fang‐Zhen , DePaolo Donald J. , Yu Xuehui , Zhu Di‐Cheng , Qi Ningyuan , Mo Xuanxue 
Published: September 2024

AbstractThe widespread Cenozoic alkaline magmatism within and around the Tibetan Plateau offers a prime opportunity to probe the nature of the mantle at the depths where basalt magmas originate. The close temporal and spatial relationship between volcanism and regional strike‐slip fault systems also helps better understand the geodynamics of outward growth of the plateau in response to the continued India‐Asia convergence. We present a comprehensive study of the deeply sourced alkaline basalts formed along the Kunlun strike‐slip fault with the aim of understanding their petrogenesis and the composition of mantle sources beneath the northeastern Tibetan Plateau. High Nb/U and Ce/Pb ratios and relatively depleted bulk‐rock Sr‐Nd‐Pb isotope compositions corroborate the mantle origin of these alkaline basalts. Homogeneous and low 87Sr/86Sr of clinopyroxene indicates negligible crustal contamination during magmatic evolution. Low δ26Mg in the alkaline basalts and positive correlations with Hf/Sm and Ti/Ti* indicate that the basalts were derived from mantle that was metasomatized by melts derived from sedimentary carbonates during the Paleo‐Tethyan seafloor subduction. Based on 40Ar/39Ar dating results, it appears that the alkaline basaltic magmatism in the northeastern Tibetan Plateau occurred simultaneously with Kunlun strike‐slip faulting. These observations suggest that the India‐Asia convergence must have reactivated ancient subduction plate boundaries and resulted in strike‐slip faulting along these suture zones within and around the Tibetan Plateau. The eruption of low‐volume and deeply rooted alkaline basalts may have been controlled by fractures associated with the strike‐slip fault systems.
A Dynamic Smagorinsky Model for Horizontal Turbulence Parameterization in Tropical Cyclone Simulation
Authors: Zhang Xu , Huang Qijun , Ma Yulong 
Published: September 2024

AbstractThe horizontal turbulence parameterization is vital for the intensity and structure forecasting of tropical cyclone (TC) in numerical weather prediction (NWP) models. The default two‐dimensional (2D) standard Smagorinsky model with a single universal constant in Weather and Research Forecasting (WRF) model has been proven to be over dissipative for TC, leading to underprediction of TC intensity. This study provides the first attempt to implement the physically based 2D dynamic Smagorinsky model (DSM) for horizontal turbulence parameterization in WRF model for TC forecasts. The DSM dynamically computes the Smagorinsky coefficient as a function of the resolved flow during the simulation, avoiding the need to prescribe the coefficient a prior. The test results of the DSM in a TC NWP model show that the DSM can significantly improve the wind intensity forecasts compared to the standard Smagorinsky model.
Rapid Ductile Strain Localization Due To Thermal Runaway
Authors: Spang A. , Thielmann M. , Kiss D. 
Published: September 2024

AbstractThermal runaway is a ductile localization mechanism that has been linked to deep‐focus earthquakes and pseudotachylyte formation. In this study, we investigate the dynamics of this process using one‐dimensional, numerical models of simple shear deformation. The models employ a visco‐elastic rheology where viscous creep is accommodated with a composite rheology encompassing diffusion and dislocation creep as well as low‐temperature plasticity. To solve the nonlinear system of differential equations governing this rheology, we utilize the pseudo‐transient iterative method in combination with a viscosity regularization to avoid resolution dependencies. To determine the impact of different model parameters on the occurrence of thermal runaway, we perform a parameter sensitivity study consisting of 6,000 numerical experiments. We observe two distinct behaviors, namely a stable regime, characterized by transient shear zone formation accompanied by a moderate (100–300 K) temperature increase, and a thermal runaway regime, characterized by strong localization, rapid slip and a temperature surge of thousands of Kelvin. Nondimensional scaling analysis allows us to determine two dimensionless groups that predict the model behavior. The ratio represents the competition between heat generation from stress relaxation and heat loss due to thermal diffusion while the ratio compares the stored elastic energy to thermal energy in the system. Thermal runaway occurs if is small and is large. Our results demonstrate that thermal runaway is a viable mechanism driving fast slip events that are in line with deep‐focus earthquakes and pseudotachylyte formation at conditions resembling cores of subducting slabs.
Defining Hydrogeophysical Layers With Multi‐Scale Geophysics for Increased Understanding of Mountain Basin Recharge
Authors: Smith E. , Carr B. 
Published: September 2024

AbstractBasin aquifers are important groundwater sources in the Western United States that are increasingly stressed due to growing populations, increased resource use, and the impacts of climate change. These aquifers are mainly recharged through melting snowpack in the surrounding mountains that infiltrates to the water table and flows directly into the basin (Mountain Front Recharge), or through deeper groundwater pathways that flow from the mountains directly into the basin aquifer (Mountain Block Recharge). However, the dominant system of recharge remains uncharacterized in many mountain basin aquifers. To address this challenge, near‐surface geophysical methods are being implemented to efficiently measure properties that govern groundwater storage and movement. This study infers groundwater storage and recharge to the Casper Aquifer around Laramie, WY, building off past studies that relied solely on sparse monitoring well data and observation of rainfall events. In this study, we use a clustering analysis on airborne electromagnetic data to define hydrogeophysical layers within the Casper Aquifer. These layers, which represent significant changes in bulk subsurface electrical resistivity, are integrated with existing hydrologic, lithologic, and smaller scale geophysical datasets to build a more representative hydrogeophysical model. Through this analysis, we define two sub‐aquifers within the larger Casper Aquifer system that are connected through structurally induced fractures and faults. This research highlights the importance of integrating geophysical data at multiple scales for defining hydrogeophysical layers that provide both a more complete understanding of basin aquifer recharge dynamics and constrain more detailed hydrologic models.
Dayside Magnetic Depression Following Interplanetary Shock Arrivals During the February 1958 and July 1959 Superstorms
Authors: Ohtani Shinichi , Odagi Yoko , Matsuoka Ayako , Iyemori Toshihiko 
Published: September 2024

AbstractThe present study investigates mid‐ and low‐latitude ground magnetic disturbances observed following the arrival of three interplanetary (IP) shocks during the super‐geomagnetic storms of February 1958 and July 1959. One may expect that after IP shocks, the H (northward) magnetic component increases globally but especially on the dayside. However, in each event, the H component was depressed sharply for 1–2 hr in the dawn‐to‐noon sector, whereas it increased in other local time (LT) sectors. Observed magnetic deflections suggest that there existed field‐aligned currents (FACs) flowing into and out of the auroral zone around the western and eastern edges of the LT sector of the dayside H depression. These features strongly suggests that the observed H depression was a remote effect of a R1‐sense FAC system. It was previously reported that similar ground magnetic disturbances were observed after the SSC of the 2003 Halloween storm, which reveals striking similarities to the well‐known H depression observed at Colaba during the 1859 Carrington storm. It is therefore suggested that the external driving behind IP shocks, especially those associated with major storms, is most optimum for the sharp reduction of the dayside H component through the formation and intensification of the dayside FAC system. Associated magnetic disturbances are considered to be larger in magnitude with increasing magnetic latitude, and oriented azimuthally as well as meridionally. Such magnetic disturbances in dayside midlatitudes may not be discussed very often as a target of space weather, but their potential impacts on ground infrastructures probably require closer attention.
Dysregulation of miRNA expression and excitation in MEF2C autism patient hiPSC-neurons and cerebral organoids
Authors: Trudler Dorit , Ghatak Swagata , Bula Michael , Parker James , Talantova Maria , Luevanos Melissa , Labra Sergio , Grabauskas Titas , Noveral Sarah Moore , Teranaka Mayu , Schahrer Emily , Dolatabadi Nima , Bakker Clare , Lopez Kevin , Sultan Abdullah , Patel Parth , Chan Agnes , Choi Yongwook , Kawaguchi Riki , Stankiewicz Pawel , Garcia-Bassets Ivan , Kozbial Piotr , Rosenfeld Michael G. , Nakanishi Nobuki , Geschwind Daniel H. , Chan Shing Fai , Lin Wei , Schork Nicholas J. , Ambasudhan Rajesh , Lipton Stuart A. 
Published: September 2024

AbstractMEF2C is a critical transcription factor in neurodevelopment, whose loss-of-function mutation in humans results in MEF2C haploinsufficiency syndrome (MHS), a severe form of autism spectrum disorder (ASD)/intellectual disability (ID). Despite prior animal studies of MEF2C heterozygosity to mimic MHS, MHS-specific mutations have not been investigated previously, particularly in a human context as hiPSCs afford. Here, for the first time, we use patient hiPSC-derived cerebrocortical neurons and cerebral organoids to characterize MHS deficits. Unexpectedly, we found that decreased neurogenesis was accompanied by activation of a micro-(mi)RNA-mediated gliogenesis pathway. We also demonstrate network-level hyperexcitability in MHS neurons, as evidenced by excessive synaptic and extrasynaptic activity contributing to excitatory/inhibitory (E/I) imbalance. Notably, the predominantly extrasynaptic (e)NMDA receptor antagonist, NitroSynapsin, corrects this aberrant electrical activity associated with abnormal phenotypes. During neurodevelopment, MEF2C regulates many ASD-associated gene networks, suggesting that treatment of MHS deficits may possibly help other forms of ASD as well.
Not too sick, not too well: reducing the diagnostic void in pediatric emergency medicine
Authors: Roland Damian , Horeczko Timothy , Snelson Edward 
Published: September 2024

AbstractEmergency clinicians must rapidly evaluate the acutely ill or injured child. In a resource-stressed environment, “spotting the sick child” is essential for appropriate stabilization, treatment, and further management. Overlooking clinical features in a child’s presentation may impede timely care. Complicating factors include the volume of patients seeking care, unfettered access to emergency services, parental perceptions and expectations, and clinician biases. Notwithstanding, after an appropriate history and physical exam, some children do not fall under the standard rubric of “sick or not sick”. This article explores strategies to recognise the child who may lie in the diagnostic void between those who are obviously well and those who are not.
Treating ICB-resistant cancer by inhibiting PD-L1 via DHHC3 degradation induced by cell penetrating peptide-induced chimera conjugates
Authors: Shi Yu-Ying , Fan Gang , Tan Ruirong , Li Shan , Sun Hua-Bing , Li Rui , Yang Mengni , Gao Shanshan , Liu Miao , Dai Meng-Yuan 
Published: September 2024

AbstractThe current selection of ligands for both proteins of interest (POI) and E3 ubiquitin ligase significantly restricts the scope of targeted protein degradation (TPD) technologies. This study introduces cell-penetrating peptide-induced chimera conjugates (cp-PCCs) targeting the DHHC3 enzyme involved in PD-L1 palmitoylation. This approach disrupts PD-L1’s immunosuppressive function, enhancing anti-tumor immunity. We developed cp-PCCs to degrade DHHC3, directly linking DHHC3-mediated PD-L1 palmitoylation to PD-L1 stability on tumor cells. Our research utilized both in vitro assays and in vivo experiments in immune checkpoint blockade-resistant mouse models. We focused on a CRBN-based cp-PCC named PCC16, which demonstrated a DC50 of 102 nmol for DHHC3 degradation and significantly reduced PD-L1 levels. In resistant models, PCC16 not only robustly downregulated PD-L1 but also exhibited substantial anti-tumor activity in vivo without significant toxicity. This outperformed traditional inhibitors, showcasing the potential of cp-PCC technology to bypass current PROTAC limitations. Our findings suggest that cp-PCCs offer a promising method for targeting PD-L1 through DHHC3 inhibition and support their continued exploration as a versatile tool in cancer immunotherapy, especially for tumors resistant to standard treatments.
The cytoplasmic-nuclear transport of DDX3X promotes immune-mediated liver injury in mice regulated by endoplasmic reticulum stress
Authors: Fan Zihao , Xu Ling , Gao Yao , Cao Yaling , Tian Yuan , Pan Zhenzhen , Wei Linlin , Chen Sisi , Zhang Xiangying , Liu Mei , Ren Feng 
Published: September 2024

AbstractImmune-mediated liver injury is a common characteristic of various liver diseases, including autoimmune and viral hepatitis. Here, we investigated the role of DEAD-box helicase 3, X-linked (DDX3X) in immune-mediated liver injury. Liver injury was induced in C57BL/6J mice via concanavalin A (Con A). DDX3X hepatocyte-specific knockout (DDX3XΔHep) mice and control (DDX3Xfl/fl) mice were utilized to investigate the role of DDX3X in liver injury. Primary hepatocytes were treated with tunicamycin (TM) to induce ER stress in vitro. The expression of DDX3X in patients with various liver diseases was evaluated. Hepatic DDX3X expression increased, and DDX3X translocated from the cytoplasm to the nucleus during Con A-induced liver injury. DDX3X deficiency ameliorated mouse liver injury and reduced ER stress in liver tissue. The inhibition of ER stress with 4-PBA significantly attenuated liver injury while decreasing DDX3X levels in liver tissue. However, the upregulation of hepatic DDX3X expression reversed Con A-induced liver injury and negated the protective effect of 4-PBA. Mechanistically, the nuclear translocation of DDX3X promoted ER stress-induced apoptosis through the transcriptional induction of CHOP. Moreover, DDX3X was elevated and translocated into the nucleus in patients with HBV-LF and AIH. Additionally, serum DDX3X levels markedly increased in patients with HBV-LF, and a consistent decrease in DDX3X was associated with a good prognosis. The cytoplasmic-to-nuclear translocation of DDX3X promotes ER stress-induced apoptosis, which is an obligatory step that drives hepatic necrosis and tissue damage. Notably, DDX3X is a potential therapeutic target for immune-mediated liver injury.
Targeted therapy of cancer stem cells: inhibition of mTOR in pre-clinical and clinical research
Authors: Son Boram , Lee Wonhwa , Kim Hyeonjeong , Shin Heungsoo , Park Hee Ho 
Published: September 2024

AbstractCancer stem cells (CSCs) are a type of stem cell that possesses not only the intrinsic abilities of stem cells but also the properties of cancer cells. Therefore, CSCs are known to have self-renewal and outstanding proliferation capacity, along with the potential to differentiate into specific types of tumor cells. Cancers typically originate from CSCs, making them a significant target for tumor treatment. Among the related cascades of the CSCs, mammalian target of rapamycin (mTOR) pathway is regarded as one of the most important signaling pathways because of its association with significant upstream signaling: phosphatidylinositol 3‑kinase/protein kinase B (PI3K/AKT) pathway and mitogen‑activated protein kinase (MAPK) cascade, which influence various activities of stem cells, including CSCs. Recent studies have shown that the mTOR pathway not only affects generation of CSCs but also the maintenance of their pluripotency. Furthermore, the maintenance of pluripotency or differentiation into specific types of cancer cells depends on the regulation of the mTOR signal in CSCs. Consequently, the clinical potential and importance of mTOR in effective cancer therapy are increasing. In this review, we demonstrate the association between the mTOR pathway and cancer, including CSCs. Additionally, we discuss a new concept for anti-cancer drug development aimed at overcoming existing drawbacks, such as drug resistance, by targeting CSCs through mTOR inhibition.
LINC00887 promotes GCN5-dependent H3K27cr level and CRC metastasis via recruitment of YEATS2 and enhancing ETS1 expression
Authors: Liao Meijian , Zheng Wendan , Wang Yifan , Li Mengting , Sun Xiaolin , Liu Nan , Yao Jia , Dong Fuxing , Wang Qingling , Ma Yu , Mou Jie 
Published: September 2024

AbstractRecent observations have revealed upregulation of H3K27cr in colorectal cancer (CRC) tissues; however, the underlying cause remains elusive. This study aimed to investigate the mechanism of H3K27cr upregulation and its roles in CRC metastasis. Clinically, our findings showed that H3K27cr served as a highly accurate diagnostic marker to distinguish CRC tissues from healthy controls. Elevated levels of LINC00887 and H3K27cr were associated with a poorer prognosis in CRC patients. Functionally, LINC00887 and H3K27cr facilitated the migration and invasion of CRC cells. Mechanistically, LINC00887 interacted with SIRT3 protein. Overexpressed of LINC00887 obstructed the enrichment of SIRT3 within GCN5 promoter, thereby elevating H3K27ac but not H3K27cr level within this region, subsequently activating GCN5 expression. This activation increased the global level of H3K27cr, promoting the enrichment of GCN5, H3K27cr, and YEATS2 within ETS1 promoter, activating ETS1 transcription and ultimately promoting the metastasis of CRC. The in vivo study demonstrated that inhibition of LINC00887 suppressed CRC metastasis, but this inhibitory effect was nullified when mice were treated with NaCr. In conclusion, our results confirmed the diagnostic biomarker potential of H3K27cr in individuals with CRC, and proposed a functional model to elucidate the involvement of LINC00887 in promoting CRC metastasis by elevating H3K27cr level.
LncRNA PVT1 induces mitochondrial dysfunction of podocytes via TRIM56 in diabetic kidney disease
Authors: Lv Zhimei , Wang Ziyang , Hu Jinxiu , Su Hong , Liu Bing , Lang Yating , Yu Qun , Liu Yue , Fan Xiaoting , Yang Meilin , Shen Ning , Zhang Dongdong , Zhang Xia , Wang Rong 
Published: September 2024

AbstractMitochondrial dysfunction is a significant contributor to podocyte injury in diabetic kidney disease (DKD). While previous studies have shown that PVT1 might play a vital role in DKD, the precise molecular mechanisms are largely unknown. By analyzing the plasma and kidney tissues of DKD patients, we observed a significant upregulation of PVT1 expression, which exhibited a positive correlation with albumin/creatinine ratios and serum creatinine levels. Then, we generated mice with podocyte-specific deletion of PVT1 (Nphs2-Cre/Pvt1flox/flox) and confirmed that the deletion of PVT1 suppressed podocyte mitochondrial dysfunction and inflammation in addition to ameliorating diabetes-induced podocyte injury, glomerulopathy, and proteinuria. Subsequently, we cultured podocytes in vitro and observed that PVT1 expression was upregulated under hyperglycemic conditions. Mechanistically, we demonstrated that PVT1 was involved in mitochondrial dysfunction by interacting with TRIM56 post-transcriptionally to modulate the ubiquitination of AMPKα, leading to aberrant mitochondrial biogenesis and fission. Additionally, the release of mtDNA and mtROS from damaged mitochondria triggered inflammation in podocytes. Subsequently, we verified the important role of TRIM56 in vivo by constructing Nphs2-Cre/Trim56flox/flox mice, consistently with the results of Nphs2-Cre/Pvt1flox/flox mice. Together, our results revealed that upregulation of PVT1 could promote mitochondrial dysfunction and inflammation of podocyte by modulating TRIM56, highlighting a potential novel therapeutic target for DKD.
Child-like sex dolls: legal, empirical, and ethical perspectives
Authors: Desbuleux Jeanne C. , Fuss Johannes 
Published: September 2024

AbstractThe review deals with the controversy surrounding the use of highly realistic dolls with a child-like appearance. It summarizes recent empirical findings and provides an overview of the different legal and ethical perspectives on this issue. Countries use different legal approaches to regulate the use or sale of child-like sex dolls. Although a causal link is assumed by some legislators between the prohibition of such dolls and the protection of children from sexual abuse, empirical studies do not support this causality. The imposition of bans will hinder empirical research on the potential use of alternative sexual outputs for people with paraphilic disorders.