bluuebunny/crossref_metadata_2025_split huggingface dataset:
Staphylococcal SSL5-induced platelet microparticles provoke proinflammatory responses via the CD40/TRAF6/NFKB signalling pathway in monocytes
Authors: Bei Jun-Jie , Liu Chuan , Peng Song , Liu Cheng-Hai , Zhao Wei-Bo , Qu Xiao-Long , Chen Qiang , Zhou Zhou , Yu Zheng-Ping , Peter Karlheinz , Hu Hou-Yuan 
Published: December 2015

SummaryPathogens-induced platelet activation contributes to inflammation in cardiovascular diseases, but underlying mechanisms remain elusive. Staphylococcal superantigen-like protein 5 (SSL5) is a known activator of platelets. Here we examined whether SSL5 is implicated in Staphylococcus aureus (S. aureus)-induced inflammation and potential mechanisms involved. As expected, we show that SSL5 activates human platelets and induces generation of platelet microparticles (PMPs). Flow cytometry and scanning electron microscopy studies demonstrate that SSL5-induced PMPs (SSL5-PMPs) bind to monocytes, causing aggregate formation. In addition, SSL5-PMPs provoke monocyte expression and release of inflammatory mediators, including interleukin-1β (IL-1β), tumour necrosis factor-α (TNFα), monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in a dose- and time-dependent manner. SSL5-PMPs also enhance MCP-1-induced monocyte migration. Blockade of CD40 and CD40 ligand (CD40L) interactions with neutralising antibodies significantly reduce monocyte release of inflammatory mediators and migration induced by SSL5-PMPs. SiRNA-mediated silencing of CD40 or TNF receptor (TNFR)-associated factor 6 (TRAF6) gene largely abrogates phosphorylation and nuclear translocation of NFkB (p65). In conclusion, SSL5 provokes the release of inflammatory mediators in monocytes, at least in part, via PMPs-mediated activation of the CD40/TRAF6/NFkB signalling pathway, though it normally inhibits leukocyte function. Our findings thus reveal a novel mechanism by which S. aureus induces inflammation.
Anti-dengue virus nonstructural protein 1 antibodies contribute to platelet phagocytosis by macrophages
Authors: Chu Ya-Ting , Lin Chiou-Feng , Chang Chih-Peng , Yeh Trai-Ming , Anderson Robert , Wan Shu-Wen , Yang Yi-Wen , Lin Yee-Shin 
Published: December 2015

SummaryThrombocytopenia is an important clinical manifestation of dengue disease. The hypotheses concerning the pathogenesis of thrombocytopenia include decreased production and increased destruction or consumption of platelets. We previously suggested a mechanism of molecular mimicry in which antibodies (Abs) directed against dengue virus (DENV) nonstructural protein 1 (NS1) cross-react with platelets. Furthermore, several lines of evidence show activation of endothelial cells (ECs) and macrophages are related to dengue disease severity. Previous studies also suggested that Ab-opsonised platelets facilitate the engulfment of platelets by macrophages. Here we show that TNF-α-activated ECs upregulate adhesion molecule expression to enhance the binding of platelets and macrophages and lead to anti-DENV NS1 Ab-mediated platelet phagocytosis. We further demonstrate that the interaction between macrophages and TNF-α-activated ECs requires binding of FcγR with the Fc region of platelet-bound anti-DENV NS1 Abs. Importantly, the binding of anti-DENV NS1 Abs to platelets did not interfere with platelet adhesion to ECs. The adhesion molecules ICAM-1 and β3 integrin expressed on ECs as well as the FcγR expressed on macrophages were critical in anti-DENV NS1 Ab-mediated platelet phagocytosis on activated ECs. Moreover, anti-DENV NS1 Abs dramatically enhanced platelet engulfment by macrophages in a murine model of DENV infection. Our study provides evidence for a novel role for anti-DENV NS1 Abs in the pathogenesis of thrombocytopenia in dengue disease by enhancing platelet phagocytosis by macrophages.
Radiographic Identification of Arthroscopically Relevant Proximal Femoral Structures
Authors: Lee W. Andrew , Saroki Adriana J. , Løken Sverre , Trindade Christiano A.C. , Cram Tyler R. , Schindler Broc R. , LaPrade Robert F. , Philippon Marc J. 
Published: December 2015

Background: Anatomic landmarks located on the proximal femur have only recently been defined, and there is a lack of radiographic guidelines for their locations presented in the literature. With the confident identification of these landmarks, radiographs could provide more assistance in preoperative evaluations, intraoperative guidance, and postoperative assessments. Purpose: To quantify the radiographic locations of endoscopic landmarks of the proximal femur. Study Design: Descriptive laboratory study. Methods: Ten cadaveric specimens were dissected, and radio-opaque hardware was placed for each landmark of interest. Radiographs were obtained and measurements recorded in anteroposterior (AP) and Dunn 45° views. Results: In the AP view, the gluteus medius insertion was located a mean 12.9 ± 2.4 mm and 34.7 ± 5.1 mm from the piriformis fossa and vastus tubercle, respectively. The piriformis fossa was a mean 14.8 ± 5.9 mm and 4.9 ± 1.9 mm from the anterior and posterior tips of the greater trochanter, respectively. The anterior and posterior tips of the greater trochanter were a mean 14.8 ± 5.1 mm from each other. In the Dunn 45° view, the piriformis fossa was a mean 13.3 ± 2.0 mm, and the vastus tubercle was a mean 21.5 ± 6.0 mm, from the gluteus medius insertion. Moreover, the vastus tubercle was a mean 33.5 ± 6.4 mm from the anterior tip of the greater trochanter and 31.6 ± 8.5 mm from the posterior tip of the greater trochanter. Conclusion: In spite of the variation in cadaveric sizes, quantitative descriptions of endoscopic landmarks were reproducible in clinical views. Clinical Relevance: A detailed understanding of how the described landmarks present radiographically is relevant to preoperative planning, intraoperative evaluations, and postoperative assessments.
Art and mental health in Samoa
Authors: Ryan Brigid , Goding Margaret , Fenner Patricia , Percival Steven , Percival Wendy , Latai Leua , Petaia Lisi , Pulotu-Endemann Fuimaono Karl , Parkin Ian , Tuitama George , Ng Chee 
Published: December 2015

Objectives: To pilot an art and mental health project with Samoan and Australian stakeholders. The aim of this project was to provide a voice through the medium of art for people experiencing mental illness, and to improve the public understanding in Samoa of mental illness and trauma. Methods: Over 12 months, a series of innovative workshops were held with Samoan and Australian stakeholders, followed by an art exhibition. These workshops developed strategies to support the promotion and understanding of mental health in Samoa. Key stakeholders from both art making and mental health services were engaged in activities to explore the possibility of collaboration in the Apia community. Results: The project was able to identify the existing resources and community support for the arts and mental health projects, to design a series of activities aimed to promote and maintain health in the community, and to pilot these programs with five key organizations. Conclusions: This project demonstrates the potential for art and mental health projects to contribute to both improving mental health and to lowering the personal and social costs of mental ill health for communities in Samoa.
Strengthening research capacity in the Pacific: an example from the Atoifi Health Research Group, Solomon Islands
Authors: MacLaren David , Asugeni James , Redman-MacLaren Michelle ,   On behalf of the Atoifi Health Research Group
Published: December 2015

Objective: To provide an example of one model of research capacity building for mental health from a remote setting in Solomon Islands. Methods: The Atoifi Health Research Group is building health research capacity with a health service on the remote east coast of Malaita, Solomon Islands. The group uses a ‘learn-by-doing’ approach embedded in health service and community-level health projects. The group is eclectic in nature and deliberately engages a variety of partners to discover culturally informed methods of collecting, analysing and disseminating research findings. Results: Key successes of the Atoifi Health Research Group are: that it was initiated by Solomon Islanders with self-expressed desire to learn about research; the learn-by-doing model; inclusion of community people to inform questions and socio-cultural appropriateness; and commitment to ongoing support by international researchers. Conclusions: Given different social, cultural, economic, geographic, spiritual and service contexts across the Pacific, locally appropriate approaches need to be considered. Such approaches challenge the orthodox approach of centralized investment to replicate specialist driven approaches of funder nations. Increasing expertise at all levels through participatory capacity building models that define and address local problems may be more sustainable and responsive to local mental health contexts.
A 13-Year Retrospective Study of Basal Cell Carcinoma in a Canadian Dermatology Practice
Authors: Rivers Jason K. , Mistry Bhavik D. , Hung Tawny , Vostretsova Kateryna , Mistry Nisha 
Published: December 2015

Background: It is unknown whether the histologic subtypes of basal cell carcinoma (BCC) arise from a common progenitor cell or whether other factors play a role in their development. Objective: To investigate the relationship between the different BCC histopathologic subtypes and anatomical distribution of BCCs in a Canadian dermatology practice. Methods: The charts of all patients diagnosed with BCC between 1993 and 2005 from a single private dermatology practice in Vancouver, Canada, were reviewed. Descriptive data analysis was undertaken to look at the distribution of histologic subtypes based on age, gender, and anatomical location. Results: Nodular BCCs accounted for 58% of all tumors. Sixty-six percent of these were situated on the head/neck (odds ratio [OR] = 3.0, 95% confidence interval [CI] = 2.1-4.3, P < .0001). Infiltrative (OR = 2.4, 95% CI = 1.5-4.1, P = .0003) and superficial BCCs were more common in women (OR = 3.7, 95% CI = 2.5-5.7, P < .0001), affected the trunk (OR = 3.2, 95% CI = 2.1-4.9, P < .0001), and appeared in younger individuals (OR = 1.8, 95% CI = 1.2-2.7, P = .004). Conclusion: Our results show a preference of distinct BCC subtypes for certain anatomical locations.
Enhanced reproducibility of SADI web service workflows with Galaxy and Docker
Authors: Aranguren Mikel Egaña , Wilkinson Mark D. 
Published: December 2015

Abstract

Background
Semantic Web technologies have been widely applied in the life sciences, for example by data providers such as OpenLifeData and through web services frameworks such as SADI. The recently reported OpenLifeData2SADI project offers access to the vast OpenLifeData data store through SADI services.

Findings
This article describes how to merge data retrieved from OpenLifeData2SADI with other SADI services using the Galaxy bioinformatics analysis platform, thus making this semantic data more amenable to complex analyses. This is demonstrated using a working example, which is made distributable and reproducible through a Docker image that includes SADI tools, along with the data and workflows that constitute the demonstration.

Conclusions
The combination of Galaxy and Docker offers a solution for faithfully reproducing and sharing complex data retrieval and analysis workflows based on the SADI Semantic web service design patterns.
Observations on the spawning behavior, egg masses and paralarval development of the ommastrephid squid Todarodes pacificus in a laboratory mesocosm
Authors: Puneeta Pandey , Vijai Dharmamony , Yoo Hae-Kyun , Matsui Hajime , Sakurai Yasunori 
Published: December 2015

ABSTRACT
The spawning behavior of ommastrephid squids has never been observed under natural conditions. Previous laboratory observations of Japanese flying squid (Todarodes pacificus) suggest that pre-spawning females might rest on the continental shelf or slope before they ascend above the pycnocline to spawn, and that the egg masses might settle in the pycnocline. Here, two mesocosm experiments were conducted in a 300 m3 tank that was 6 m deep to investigate this hypothesis. In the first experiment, a thermocline (2.5–3.5 m) was established in the tank by creating a thermally stratified (17–22°C) water column. In the second experiment, the temperature was uniform (22°C) at all depths. Prior to spawning, females did not rest on the tank floor. In the stratified water column, egg masses remained suspended in the thermocline, but in an unstratified water column, they settled on the tank bottom, collapsed and were infested by microbes, resulting in abnormal or nonviable embryos. Eleven females spawned a total of 18 egg masses (17–80 cm in diameter), indicating that females can spawn more than once when under stress. Paralarvae hatched at stage 30/31 and survived for up to 10 days, allowing us to observe the most advanced stage of paralarvae in captivity. Paralarvae survived after consumption of the inner yolk, suggesting they might have fed in the tank.
Surface-Assisted Laser Desorption Ionization Mass Spectrometry (SALDI-MS) of Low-Molecular-Weight Medicines and Toxic Materials Using Commercial TiO2 Nanoparticles
Authors: Yonezawa Tetsu , Asano Takashi , Matsubara Masaki 
Published: December 2015

Abstract
The ability of surface-assisted laser desorption/ionization mass spectroscopy (SALDI-MS) of low-molecular-weight compounds using six types of commercially available TiO2 nanoparticles (NPs) was examined. In all cases, TiO2 NPs gave strong Na+-adduct sample ion peaks. Some chemicals, which rarely show proton-adduct sample ion peaks by MALDI-MS, such as aspirin and barbital, could be easily detected by using TiO2 NPs. The key factors determining the SALDI ability of TiO2 NPs are their purity, ratio of rutile, and particle size.
Diagnosis and therapy in cancer-associated thromboembolism – what about guideline recommendations?
Authors: Matzdorff A. 
Published: December 2015

SummaryOncogenic transformation is closely linked to coagulation activation and cancer-associated venous thromboembolism (VTE) is a common problem. Guidelines recommend thromboprophylaxis with a low molecular weight heparin for hospitalized cancer patients. However, thromboprophylaxis is not customarily advised for ambulatory cancer patients. Cancer patients with VTE are usually treated with a low molecular weight heparin for 3–6 months. Vitamin K antagonists have a higher bleeding risk and there are not sufficient data to recommend any of the new oral anticoagulants. All physicians taking care of cancer patients should be aware of the current guideline recommendations. Oncology professionals should educate patients about the signs of VTE.
Heparins – DOACS – VKA
Authors: Schinzel H. 
Published: December 2015

SummaryPatients with cancer are at increased risk of venous thromboembolism (VTE). At the same time they have often an underlying bleeding risk. That can often make decisions surrounding the administration of anticoagulants complicate. Individual risk-benefit calculation is necessary. During hospital stage the patients get, if there are no contraindications, a medical VTE prophylaxis with low molecular weight heparin (LMWH). Whereas out-patients don’t get a prophylaxis because they are at low risk of thromboembolism. If additional risk factor for VTE exists a decision for medical VTE prophylaxis should be taken into account. In patients with cancer and acute VTE, LMWH is recommended as treatment of choice for initial and long-term management in a body weight adapted dosage. After a period of 3–6 month and if a prolonged treatment is necessary, guidelines allow to switch from LMWH to VKA for further anticoagulant therapy. Beside the established anticoagulants like heparin, vitamin K antagonists, fondaparinux new oral direct anticoagulants (DOACs) were established in the last years. These substances are evaluated in in clinical trials. They are approved for treatment of acute VTE, for secondary prophylaxis and for prevention of ischemic stroke in patients with arterial fibrillation. In the VTE trials, 4–10 % of the enrolled patients had a history of cancer. The data shows that DOACs can prevent recurrent VTE as good as standard therapy with enoxaparin/warfarin without more bleeding complications. The results are encouraging. Because of the limited data the direct oral anticoagulants are not recommended for treatment of VTE at this time. Further studies are necessary.
Atypical venous thromboses in myeloproliferative neoplasias
Authors: Dicke C. , Frölich A. , Holstein K. , Bokemeyer C. , Langer F. 
Published: December 2015

SummaryWe describe two patients who developed an extensive catheter-related upper extremity deep vein thrombosis and a cerebral venous sinus thrombosis, respectively. Both thrombotic complications occurred in the presence of an elevated platelet count. Subsequent detection of the JAK2 V617F and MPL mutations led to the diagnosis of a myeloproliferative neoplasia.
Situational Assignments in the Ecological Education of Primary School Children
Authors: Гринёва  , Grineva E. , Давлетшина  , Davletshina L. 
Published: December 2015

The paper considers the issue on how to unlock the spiritual and moral potential of primary school ecological education, based on learning
through the use of situational assignments. The authors provide typology of situations (cases), designed to facilitate pupils’ spiritual development.
Examples on each type of situational assignments are drawn.
The Economic Component of the «World Around Us» Subject (2nd and 3rd Grades)
Authors: Корнеева  , Korneeva Olga 
Published: December 2015

The paper covers the prospects of economic education of primary school pupils and considers economic concepts included in the of the “World
Around Us” subject curriculum. Also provided are didactic materials for class work, designed to help primary school pupils to learn and deepen
their economic knowledge and to encourage their interest in the economic issues.
Forming an Algorithm for Determining the Grammatical Gender of Nouns (The Russian Language Lesson at the Third Grade of Primary School)
Authors: Хотько  , Khotko E. 
Published: December 2015

The paper considers the fair culture as being the element of the Russian vernacular and festive folklore, which for the whole existence of domestic
education has always used to be a means of upbringing the youth. Acquaintance of schoolchildren with the national folklore helps to aspire their
interest in the national traditions and history; to engage them with the origins of the Russian culture. As the author proves, the folklore material
can easily be used within the modern primary education to stage mass cultural events and also in the course of after-school activities.
Afars, Issas … and Djiboutians: Toward a History of Denominations
Authors: Imbert-Vier Simon 
Published: December 2015

Abstract
This article analyzes the ethnic denominations around the Gulf of Tadjoura. By using travel narratives from the nineteenth century and colonial archives from the twentieth, it provides a history of these denominations, their construction and evolution, and the representations they carry. In the nineteenth century, denominations proposed by African informers were used by European travelers to describe the political and social situation in the area as they understood it. Later, the colonial administration wanted to identify groups and individuals in order to manage the inhabitants and secure its domination over the country. We show how this practice was an impossible task because of the identity lability of individuals and groups and the impossibility of defining accurate limits between them, either physical or symbolic. Nonetheless, these constructions have been used until now to legitimate access to the State and country's resources.
Error and misconception: Relation of fraction and part-whole<p>Hata ve kavram yanılgısı: Kesir ve parça bütün ilişkisi
Authors: Altıparmak Kemal 
Published: December 2015

<p>In this study, students’ fraction concept has been studied to reveal their errors and misconceptions. For this purpose, researchers prepared an “error and misconception diagnostic test” which consists of 34 questions about part-whole relationship (simple and compound fractions), number line, and comment. The reliability coefficient of this test is 0.86. The misconceptions diagnostic test was applied to 73 secondary school students and 113 university students. According to results, students participated in the study had five different misconceptions type about fractions. They are: Unequal partitioning misconceptions; misconceptions about the expansion and simplification of fractions; misconceptions resulting from conceiving number line in part-whole relationship; misconceptions because of using unequal parts of a whole while adding; misconceptions about adding numerators and denominators of fractions.</p><p> </p><p><strong>Özet</strong></p><p>Bu çalışmada öğrencilerin kesir konusundaki hata ve kavram yanılgıları ortaya çıkarılmaya çalışılmıştır. Bu amaçla 37 soruluk “hata ve kavram yanılgıları teşhis testi” hazırlanmıştır. Bu test kesir konusu için parça-bütün, sayı doğrusu, yorum kısmından oluşmaktadır. Bu testin güvenilirlik katsayısı 0.86 bulunmuştur. Hata ve kavram yanılgıları teşhis testi 73 ortaokul, 113 üniversite öğrencisine uygulanmıştır. Sonuçlara göre, öğrenciler 5 tipte kavram hatasına sahiptirler. Bunlar sırasıyla; bir bütünün eş olmayan parçalara ayrılması ile ilgili kavram hatası, Parça bütün üzerinde genişletme ve sadeleştirme konusunda kavram yanılgısı, Sayı doğrusunu parça bütün olarak görme konusundaki kavram yanılgısı, Toplama işlemi için eş olmayan bütünlerin kullanılması üzerine kavram yanılgısı, Paydası eşit olmayan kesirlerde toplama yapılırken paylar toplanıp paya, paydalar toplanıp paydaya yazılan kavram hatasıdır<em>.</em></p>
“Oh My Gosh! The Expression Is Too Chinese”: Attitudes of University Teachers and Students towards China English
Authors: Wang Weihong , Gao Xuesong 
Published: December 2015

AbstractWith the wide spread of English in the global context, scholars increasingly call for the reshaping of English education towards teaching English as an international language. One oft-mentioned proposal is to increase students’ exposure to more English varieties, in particular the variety of English used in their country or region. Against this backdrop, this study investigated the attitudes of Chinese university students and teachers towards China English (CE), an emerging variety in China and the ideological underpinnings beneath their attitudinal responses. In the study, 33 English teachers and 31 students were asked to first evaluate the understandability and acceptability of CE features that are identified in literature and then provide reasons justifying their choices in a questionnaire survey. Results show that both teacher and student participants were reluctant to accept CE as a legitimate variety but their attitudes diverged as regards specific CE features. The in-depth exploration of their justifications revealed that native speaker English ideology, Chinglish stigma and the communicativeness of CE to the outside world were their major concerns in evaluating CE. Findings in this study have implications for educators who attempt to localize English language teaching.