bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Mechanism Underlying Radiotherapy Resistance
Caused by Oligodendroglia Cells in Glioblastoma by Applying the
Single-cell RNA Sequencing Technology
Authors: Yuan Qinghua , Gao Weida , Guo Mian , Liu Bo 
Published: September 2024

Background:
Glioblastoma (GBM) is an aggressive malignancy. The inherent resistance of GBM to radiotherapy poses great challenges for clinical treatment

Objectives:
The primary objective of this study is to explore the molecular mechanisms
of radiotherapy resistance in GBM and identify the key influencing factors that contribute to this phenomenon.

Methods:
The single-cell RNA sequencing (scRNA-seq) data of GBM were downloaded from the Gene Expression Omnibus (GEO) database. Cells were clustered using the
Seurat R package, and the clusters were annotated using the CellMarker database. Pseudotime analysis was conducted using Monocle2. Marker scores were calculated based
on the RNA-seq data of GBM from the UCSC database, and the enrichment of Hallmark
gene sets was measured with the AUCell package. Furthermore, the most frequently mutated genes were identified using the simple nucleotide variation data from The Cancer
Genome Atlas (TCGA) applying the maftools package.

Results:
This study identified two oligodendrocyte subsets (ODC3 and ODC4) as radiotherapy-resistant groups in GBM. Enrichment and Pseudotime analysis revealed that the
inflammatory response and immune activation pathways were enriched in ODC3, while
the cell division and interferon response pathways were enriched in ODC4. The enrichment scores of hallmark gene sets further confirmed that ODC3 and ODC4 subpopulations developed radiotherapy resistance via distinct molecular mechanisms. Analysis of
gene mutation frequencies showed that TP53 exhibited the most significant change in
mutation frequency, indicating that it was an important risk factor involved in radiotherapy resistance in GBM.

Conclusion:
We identified two ODC subpopulations that exhibited resistance to radiotherapy, providing a new perspective and potential targets for personalized treatment
strategies for GBM.
DeepLCRmiRNA: A Hybrid Neural Network Approach for Identifying
Lung Cancer-Associated miRNAs
Authors: Cheng Nitao , Chen Chen , Liu Junliang , Wang Xuanchun , Gao Ziqi , Mao Ming , Huang Jingyu 
Published: September 2024

Introduction:
Lung cancer stands as one of the most prevalent malignant neoplasms,
with microRNAs (miRNAs) playing a pivotal role in the modulation of gene expression, impacting
cancer cell proliferation, invasion, metastasis, immune escape, and resistance to therapy.

Method:
The intricate role of miRNAs in lung cancer underscores their significance as biomarkers
for early detection and as novel targets for therapeutic intervention. Traditional approaches for the
identification of miRNAs related to lung cancer, however, are impeded by inefficiencies and complexities.

Results:
In response to these challenges, this study introduced an innovative deep-learning strategy
designed for the efficient and precise identification of lung cancer-associated miRNAs. Through
comprehensive benchmark tests, our method exhibited superior performance relative to existing
technologies.

Conclusion:
Further case studies have also confirmed the ability of our model to identify lung cancer-associated miRNAs that have undergone biological validation.
The Efficacy and Safety Analysis of the Bendamustine, Pomalidomide, and Dexamethasone (BPD) Combination Treatment for Patients with Proteasome Inhibitor Intolerance and/or Relapsed Multiple Myeloma
Authors: Zhao Yiming , Tian Wanlu , Jin Feng bo , Jiang Lei , Zhang Rui , Qian Wei , Xia Leiming , Yang Ming zhen 
Published: September 2024

Background:
Multiple Myeloma (MM) is a hematologic malignancy that often progresses
to a refractory relapse, posing significant treatment challenges due to prior treatments,
drug response duration, clinical and molecular characteristics, comorbidities, and adverse reactions.

Methods:
This single-center and single-arm study assessed the BPD regimen, which includes bendamustine,
pomalidomide, and dexamethasone, for its efficacy and safety in 21 patients with Relapsed
and Refractory Multiple Myeloma (RRMM), including those who were intolerant to prior
bortezomib treatment.

Results:
The Overall Response Rate (ORR) after 1-8 cycles of BPD treatment was 58.8%. The 6--
month Progression-Free Survival (PFS) was 70.5%, and the 12-month PFS was 52.9%. The 1-year
Overall Survival (OS) rate was 82.35%. Hematologic toxicities were the main adverse reactions,
with grade 3 or higher adverse events mainly linked to hematologic toxicity and infections.

Conclusion:
The BPD regimen has shown to be highly effective, with a favorable ORR and survival
rate in RRMM patients, indicating it a relatively safe and well-tolerated treatment option.
Influence of Surgical Mask on Exertion Perception and Select Physiological Parameters During an Incremental Test in Healthy Subjects
Authors: Piñeros-Alvarez Jose Luis , Portuguez-Jaramillo Nelson Esteban , Rosero- Carvajal Hamilton Elias , Pizarro-Loaiza Mayra Liliana , Murillo-Ortiz Monica Andrea , Tonguino-Rosero Stefanie 
Published: September 2024

Background:
During cardiac or pulmonary rehabilitation exercises, the use of a surgical
mask may not be well tolerated by patients, especially those experiencing dyspnea during certain
activities due to their underlying health conditions. The purpose of this study was to describe
the influence of a surgical mask on exertion perception and some physiological parameters during
an incremental test in healthy subjects.

Methods:
A descriptive study involving 16 healthy adult individuals was conducted. The BRUCE
incremental protocol was performed twice, with and without the use of a surgical mask.

Results:
No significant changes were found in vital signs, such as heart rate (p: 0.8684), mean arterial
pressure (p: 0.2781), and peripheral oxygen saturation (p: 0.5314). However, the respiratory
rate showed a significant difference (p: 0.023). Furthermore, significant differences were observed
in the distance covered (p: 0.0005), duration (p: 0.0003), and measured intensity (METs)
(p: 0.0117). Metabolic acidosis (p: 0.032), hypocapnia (p: 0.238), and hyperlactatemia (p: 0.024)
were detected in the analysis of arterial gases. Oxygenation parameters, such as PaO2 (p: 0.038)
and PaO2/FiO2 ratio (p: 0.038), were higher in the mask-off trial.

Conclusion:
Surgical masks induce negative changes in METs, distance covered, and duration
during an incremental treadmill test. Although no significant impact on vital signs was observed,
the use of a surgical mask resulted in statistically significant but not clinically relevant decreases
in oxygenation parameters. The stages of the Bruce protocol were achieved differently when participants
wore a surgical mask.

conclusion:
During a treadmill incremental test, surgical masks induce negative changes in MET, distance covered, and duration. Although no significant impact was observed on vital signs, wearing a surgical mask resulted in statistically significant but not clinically relevant decreases in oxygenation parameters. The stages of the Bruce protocol were achieved differently when participants wore a surgical mask.
Biochemical Sources of Fulvic Acid and its Application in Agriculture
Authors: Chen Yu-He , Liu Jialei , Liu Yang , Liu Zun-Qi 
Published: September 2024

:
The demand for crop yields is increasing in modern society. The challenges to agricultural
cultivation, nevertheless, are posed by soil degradation, pollution, and the increasing number
of extreme weather events due to climate change. All these factors can threaten crop yield and quality.
However, fulvic acid can effectively alleviate these problems. It’s a type of humus with multiple
agricultural values, is produced through biomass fermentation, and has various mechanisms that
promote crop growth. It plays a significant role in mitigating various adverse factors affecting crop
growth. Fulvic acid contains more carboxyl and hydroxyl groups but also the presence of ether
bonds and ester bonds, due to the special structure of xanthic acid, which also allows it to closely
bond with many metal ions and substances. This paper provides an in-depth study of the mechanism
and molecular structure of fulvic acid production and describes a variety of application scenes of
fulvic acid in agricultural production. This paper makes a general conclusion on the improvement
of yield and quality of yellow rot crops and analyzes the resistance of yellow rot to the adverse environment,
including heavy metal hazards, salt stress hazards, drought hazards, pests, and diseases.
Through the analysis by many scholars, yellow rot acid can play a significant role in the improvement
of the adverse environment growing plants.

:
Furthermore, the combined use of fulvic acid and modern drip irrigation techniques is receiving
increased attention due to the significant benefits it offers in terms of water management and enhanced
fertilizer efficiency. More studies should be conducted on the mechanism of xanthate in
plants. The characterization of fulvic acid properties is indispensable in practical agricultural applications.
Moreover, the development of effective fulvic acid derivatives according to the actual situation
is essential to promote the sustainable development of agriculture.
In Silico Evaluation of Novel Quinoline Derivatives Targeting Hepatocyte Growth Factor Receptors as Anticancer Agents
Authors: Mhaske Ganesh , Rokade Siddhesh , Rokade Chaitrali , Sonkusare Diksha , Mane Divyani , Mane Poonam , Shinde Aishwarya 
Published: September 2024

Background:
Numerous methods for computer-aided drug design (CAAD) have made
it possible to create and synthesize new chemical entities. The utilization of in silico techniques
and structure-based drug design (SBDD) facilitate the visualization of the ligand-target binding
process, in addition to allowing the prediction of receptor affinities and important binding pocket
locations.

Objective:
The current research work was carried out to recognize novel quinoline derivatives designed
specifically to bind with hepatocyte growth factor (HGF) receptors.

Materials and Methods:
For the formation of quinolines derivatives, ChemAxon Marvin Sketch
5.11.5 was utilized. SwissADME and the admetSAR online web tools were exploited to predict
the pharmacokinetic properties and the toxicity of compounds. Numerous software, including Autodock
1.5.7, MGL Tools 1.5.7, Biovia Discovery Studio Visualizer v20.1.0.19295, Procheck,
Protparam tool, and PyMOL, were also used to determine the ligand-receptor interactions of derivatives
of quinoline with the target receptor (PDB -1R0P).

Results:
Based on in silico research, it was found that all compounds were less toxic, orally bioavailable,
and had the proper pharmacokinetic properties. When compared to the commonly used
drug gefitinib, the docking scores of all newly created derivative compounds were higher.

Conclusion:
An increased binding energy, the number of H-bonds generated, and interactions
with quinoline analogues are significant parameters to be considered while constructing compounds
that are most appropriate for additional investigation. The favorable pharmacokinetic profile
of quinoline moiety was found to enhance its potential as a novel lung cancer treatment alternative
and may help medicinal chemists to carry out more thorough in vitro, in vivo, chemical, and
pharmacological research studies.
Protective Role of Ovothiol-A against Muscle and Kidney Injuries in Obese Rats
Authors: Arafa Nada Hussien , Shehata Mohamed Refaat , Mohamed Ayman Saber 
Published: September 2024

Background:
Obesity is a serious chronic metabolic disease impairing health damaging
many organs such as kidneys and muscles. Ovothiol-A (Ovo-A) has been found to keep the redox
balance normal in sea urchins indicating its antioxidant characteristics.

Aim:
This study aims to investigate the protective effects of Ovo-A on kidneys and muscles in
obese rats.

Methods:
In-silco studies were performed on lactate dehydrogenase (LDH) and creatine kinase
(CK) with Ovo-A to compute their binding affinities. Obesity was induced by high-fat diet (HFD)
for 4 weeks. Wistar rats were used in this study as 6 rats per group as control, HFD, Ovo-A (200
and 400 mg/Kg, p.o) groups.

Results:
Docking results have revealed that Ovo-A has affinities to bind to LDH (-8.5 kcal/mol)
and CK (-17.7 kcal/mol). Ovo-A reduced the levels of uric acid, urea, creatinine, LDH, CK,
malondialdehyde (MDA), and nitric oxide (NO), while increasing the levels of glutathione (GSH),
catalase (CAT), and glutathione-S-transferase (GST). Histopathological investigations have revealed
that Ovo-A restored the renal and muscular structure.

Conclusion:
The current study showed that Ovo-A has a protective effect on kidneys and muscles
in obese rats. Ovo-A enhances renal and muscular functions by inhibiting LDH and CK activities
and improving the antioxidant system. Ovo-A is more effective in the high dose.
Unveiling The Potential of Microwave Plasma Treated Char For Sustainable Automotive Shredder Residue (ASR) Management and Reduced Ecological Risk Of Cadmium
Authors: Ngo Thi-Huyen-Tran , Wang Ya-Fen , Chang Tien-Chin , You Sheng-Jie 
Published: September 2024

Introduction:
This study explores the effectiveness of microwave plasma pyrolysis in reducing
heavy metal concentrations, specifically cadmium, in automobile shredder residue (ASR).
METHOD: The ASR was subjected to plasma pyrolysis at microwave power levels ranging from
800 to 1000W, resulting in a significant decrease in heavy metal concentration in the char. Utilizing
the Bureau Communautaire de Référence (BCR) sequential extraction method, a standardized procedure
developed by the European Commission, we assessed the chemical speciation and matrix
properties of the char. The results indicated that microwave plasma treatment effectively decreased
the bioavailable forms of cadmium. After 6 minutes of pyrolysis at 1000W, the exchangeable and
acid-soluble fractions (F1) dropped to 10%, while the reducible fraction (F2) fell to 12%. RESULT:
This treatment resulted in a substantial increase in the oxidizable (F3) and residue (F4) fractions,
which reached 27% and 51%, respectively. Importantly, the potential ecological risk of cadmium
decreased significantly, with the risk index (Er) dropping from 164.91 to 28.45, aligning with Taiwan's
regulatory standards for non-hazardous waste.CONCLUSION: These findings suggest that
microwave plasma pyrolysis is a promising and sustainable approach for the disposal of ASR, offering
an environmentally friendly alternative for waste management. This study provides valuable
insights for policymakers, environmental scientists, and industries seeking effective solutions for
ASR treatment.
TÉCNICAS MULTIVARIADAS NA DETERMINAÇÃO DA DIVERSIDADE DE MANDIOCA NO SUL MARANHENSE
Authors: Clemilton Alves da Silva  , Dávila da Silva Santos  , Joyce de Sousa Cordeiro  
Published: September 2024

As técnicas multivariadas, aplicadas em várias áreas do conhecimento, são amplamente utilizadas em estudos genéticos devido à possibilidade de integrar simultaneamente, múltiplas informações e quantificar a variância de caracteres de interesse agronômico. Objetivou-se caracterizar a variabilidade de genótipos mandioca cultivada no sul maranhense, por meio técnicas multivariadas, considerando dez genótipos e dezessete descritores morfológicos. Por meio da utilização dos diferentes critérios de agrupamento, pode-se identificar a existência de agrupamento natural dos genótipos, em função da similaridade genética do germoplasma avaliado. Foi constatado a existência de variabilidade genética entre os genótipos mandioca estudados, sendo o genótipo Formosa, os mais divergentes constatado pelos diferentes métodos utilizados. Ademais, com base nos resultados obtidos, espera-se que o cruzamento realizado entre um dos genótipos mais divergentes com quaisquer outros materiais pertencentes aos demais grupos, apresente características de importância agronômica.
A Suicide Prevention Digital Technology for Individuals Experiencing an Acute Suicide Crisis in Emergency Departments: Naturalistic Observational Study of Real-World Acceptability, Feasibility, and Safety
Authors: Dimeff Linda A , Koerner Kelly , Heard Kandi , Ruork Allison K , Kelley-Brimer Angela , Witterholt Suzanne T , Lardizabal Mary Beth , Clubb Joseph R , McComish Julie , Waghray Arpan , Dowdy Roger , Asad-Pursley Sara , Ilac Maria , Lawrence Hannah , Zhou Frank , Beadnell Blair 
Published: September 2024

Background
Emergency departments (EDs) are the front line in providing suicide care. Expert consensus recommends the delivery of several suicide prevention evidence-based interventions for individuals with acute suicidal ideation in the ED. ED personnel demands and staff shortages compromise delivery and contribute to long wait times and unnecessary hospitalization. Digital technologies can play an important role in helping EDs deliver suicide care without placing further demands on the care team if their use is safe to patients in a routine care context.

Objective
This study evaluates the safety and effectiveness of an evidence-based digital technology (Jaspr Health) designed for persons with acute suicidal ideation seeking psychiatric crisis ED services when used as part of routine ED-based suicide care. This study deployed Jaspr Health for real-world use in 2 large health care systems in the United States and aimed to evaluate (1) how and whether Jaspr Health could be safely and effectively used outside the context of a researcher-facilitated clinical trial, and (2) that Jaspr’s use would be associated with improved patient agitation and distress.

Methods
Under the auspices of a nonsignificant risk device study, ED patients with acute suicidal ideation (N=962) from 2 health care systems representing 10 EDs received access to Jaspr Health as part of their routine suicide care. Primary outcome measures included how many eligible patients were assigned Jaspr Health, which modules were assigned and completed, and finally, the number of adverse events reported by patients or by medical staff. Secondary outcome measures were patient agitation, distress, and satisfaction.

Results
The most frequent modules assigned were Comfort and Skills (98% of users; n=942) and lethal means assessment (90% of patient users; n=870). Patient task completion rates for all modules ranged from 51% to 79%. No adverse events were reported, suggesting that digital technologies can be safely used for people seeking ED-based psychiatric services. Statistically significant (P<.001) reductions in agitation and distress were reported after using the app. Average patient satisfaction ratings by site were 7.81 (SD 2.22) and 7.10 (SD 2.65), with 88.8% (n=325) and 84% (n=90) of patients recommending the app to others.

Conclusions
Digital technologies such as Jaspr Health may be safely and effectively integrated into existing workflows to help deliver evidence-based suicide care in EDs. These findings hold promise for the use of digital technologies in delivering evidence-based care to other vulnerable populations in complex environments.
Sodium-Glucose Cotransporter 2 Inhibitors and Kidney Outcomes across the Spectrum of Kidney Disease
Authors: Spiazzi Bernardo F. , Piccoli Giovana F. , Wayerbacher Laura F. , Lubianca João Pedro N. , Scalco Bruno G. , Scheffler Mariana H. , Fraga Bruna L. , Colpani Verônica , Gerchman Fernando 
Published: September 2024

Key Points

The effect of sodium-glucose cotransporter 2 inhibitors in preventing kidney outcomes in populations at lower risk of kidney disease remains uncertain.Pooled data from randomized controlled trials show that sodium-glucose cotransporter 2 inhibitors prevent kidney outcomes across the spectrum of kidney disease risk.

Background
Sodium-glucose cotransporter 2 (SGLT2) inhibitors have shown to reduce clinically meaningful kidney outcomes in individuals with CKD at high risk of adverse outcomes. The effect of these agents in preventing clinically meaningful kidney outcomes in populations at lower risk remains uncertain. We aim to evaluate the effect of SGLT2 inhibitors on kidney outcomes across the Kidney Disease Improving Global Outcomes (KDIGO) classification and urinary albumin-creatinine ratio (UACR) levels.

Methods
We have searched medical literature analysis and retrieval system online (PubMed), excerpta medica database, and Cochrane Central Register of Controlled Trials from inception up to August 8, 2023. In pairs, researchers selected large (≥500 participants per arm) randomized placebo-controlled trials of SGLT2 inhibitors, with a minimum duration of 1 year. Researchers independently extracted study-level data and assessed within-study risk of bias with the risk of bias 2.0 tool and quality of evidence with grading of recommendations, assessment, development and evaluation.

Results
We included ten trials, encompassing 78,184 participants and a median follow-up of 2.7 years. Risk of bias was overall low. We performed meta-analyses summarizing individual study hazard ratios (HRs) and 95% confidence intervals (CIs) using a random-effects model. SGLT2 inhibitors reduced the composite kidney outcome across all KDIGO (HR [95% CI]: low 0.48 [0.32 to 0.71], moderate 0.60 [0.39 to 0.93], high 0.59 [0.47 to 0.74], very high 0.59 [0.49 to 0.72]) and UACR (HR [95% CI]: <30 mg/g 0.62 [0.50 to 0.78], ≥30 to ≤300 mg/g 0.80 [0.67 to 0.96], >300 mg/g 0.61 [0.52 to 0.73]) groups, without evidence of heterogeneity between groups. A small proportion of participants without diabetes in low-risk groups were referred, and there was lack of standardization of composite outcomes.

Conclusions
SGLT2 inhibitors consistently reduce kidney outcomes across the spectrum of KDIGO classes and UACR levels.

Clinical Trial registry name and registration number:
CRD42023492877.