bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vicious circle of peripheries: Representation of sub‐municipal units in local election manifestos in amalgamated municipalities
Authors: Voda Petr , Vodová Petra 
Published: October 2024

AbstractMerged municipalities create unequal opportunities for sub‐municipal units according to their relative size and administrative centrality. Because electoral manifestos provide the basis for policy‐making on services, we analyse how the mentions of different sub‐municipal units are distributed in manifestos. We analyze the manifestos of 381 parties in 82 amalgamated municipalities in the 2022 local elections in Czechia, focussing on the effects of size, administrative centrality, and presence of candidates from the units on candidate lists. The probability of mentioning a unit is affected by the presence of candidates from the unit on candidate lists, and that the probability of mentioning is very low for small and peripheral units without candidates. The results show the systematic differences in attention parties pay to various sub‐municipal units and crucial importance of unit‐based candidates for the substantive representation within the amalgamated municipalities.
Clinical application of centrifugal‐membrane hybrid plasmapheresis in the treatment of hyperlipidemia
Authors: Yi Zhongmei , Wu Chunxi , Zhou Yumeng , Zhang Bin 
Published: October 2024

AbstractObjectiveThis study aimed to clarify the clinical application of centrifugal‐membrane hybrid plasmapheresis (CMHP) in the treatment of hyperlipidemia.MethodsA retrospective study was conducted on 48 patients who were diagnosed with hyperlipidemia and had received CMHP treatment. Serum total cholesterol (TC), triglycerides (TG), high‐density lipoprotein cholesterol (HDL‐C), and low‐density lipoprotein cholesterol (LDL‐C) were monitored, and adverse reactions to the treatment were observed.ResultsForty‐eight patients with hyperlipidemia received CMHP over 59 sessions. The average age of the 48 patients with hyperlipidemia, including 32 males (66.67%) and 16 females (33.33%), was 44.23 ± 12.02 years. Twenty‐nine outpatients (60.42%) and 19 inpatients (39.58%) were included. Hypertriglyceridemia was diagnosed in 16 cases (33.33%), mixed hyperlipidemia in 31 cases (64.58%), and hypercholesterolemia in one case (2.08%). The pretreatment blood lipid concentrations were significantly different after the 59 CMHP treatments (p < .001). The concentrations of TC, TG, HDL‐C, and LDL‐C decreased significantly after the treatment, and the median ratios of reduction were 67.06% (range: 58.97%–71.87%), 63.33% (range: 55.20%–74.86%), 45.87% (range: 35.86%–52.95%), and 66.09% (range: 44.37%–73.94%), respectively. Three adverse reactions (5.08%) were recorded. No differences were detected in therapeutic parameters, effects, or adverse reactions between the two blood cell separators, there was no difference in Lipoprotein apheresis efficacy.ConclusionThis preliminary study demonstrated the clinical application of CMHP in patients in the treatment of hyperlipidemia. However, further studies are needed applying CMHP with hyperlipidemia.
Clinical Utility of Circulating Cell‐Free DNA as a Liquid Biopsy in Cats With Various Tumours
Authors: Tagawa Michihito , Hiroi Hotaka , Nakano Yuzuki , Morishita Riyo , Kobayashi Kosuke , Sakai Osamu 
Published: October 2024

ABSTRACTOnly a limited number of tumour biomarkers are currently available in veterinary medicine, particularly in cats. Cell‐free DNA (cfDNA) is an extracellular DNA fragment released upon cell death and is considered a minimally invasive biomarker for the diagnosis and monitoring of various human malignancies. This study aimed to clarify the utility of circulating cfDNA as a liquid biopsy for various feline tumours. Plasma samples were collected from 44 cats with various tumours, 24 cats with other diseases and 10 healthy controls. A follow‐up study was conducted in three tumour‐bearing patients. All cfDNA concentrations were quantified via real‐time polymerase chain reaction (PCR), which provided short and long fragments of a newly identified feline LINE‐1 gene. We found that cfDNA levels were significantly higher in cats with various tumours than in those with other diseases or healthy controls. The cfDNA concentration was not correlated with serum amyloid A (SAA) levels. Cats with tumours exhibited elevated cfDNA levels that predicted tumour‐bearing with a sensitivity and specificity of 50.5% and 91.2%, respectively (AUC 0.736; p < 0.001). In lymphoma cases, cats with high cfDNA levels had significantly shorter survival times than those with low cfDNA levels (median: 33 days vs. 178 days; p = 0.003). In addition, the cfDNA levels of the three patients correlated with clinical status during follow‐up. Collectively, these findings indicate the potential of cfDNA as a useful biomarker for the diagnosis, therapeutic monitoring and prognostic assessment of tumours in cats.
Multicentric aggressive unclassified hematopoietic neoplasm involving the placenta in a pregnant bitch
Authors: Marconato Laura , Sabattini Silvia , Zambelli Daniele , Ferrari Maria Giulia , Aresu Luca , Renzi Andrea , Ferrari Anna , Cunto Marco , Maga Ilaria , Ballotta Giulia 
Published: October 2024

AbstractHematopoietic neoplasms are common in dogs; however, their association with pregnancy has not been previously reported in veterinary medicine. This rare occurrence presents a variety of diagnostic, therapeutic, prognostic, and ethical challenges. We report a case of a 3‐year‐old pregnant Bernese Mountain Dog diagnosed with multicentric aggressive unclassified hematopoietic cancer associated with paraneoplastic hypercalcemia during pregnancy. The dog died 7 days after diagnosis, and at Day 36 of pregnancy before any treatment decision could be made. Post‐mortem evaluation, including histology, immunohistochemistry, and clonality analysis, led to the diagnosis of an unclassified hematopoietic cancer affecting the uterus and placenta, with no evidence of fetal involvement. The placenta likely acted as a barrier, preventing neoplastic involvement of the fetuses. Alternatively, the pregnancy might have been too early for the hematopoietic neoplasm to affect the labyrinth zone of the placenta and the fetuses. The dramatic disease progression could be explained by compromised cell‐mediated immunity during pregnancy. This immunodeficient state is induced by embryonic, maternal, and hormonal factors, which suppress the response to mitogens to prevent rejection of the placenta and the conceptuses. Thus, pregnant dogs might exhibit increased vulnerability to cancer and infectious diseases that rely on cell‐mediated immunity for host defense.
The effect of midline shift on survival time in dogs with structural brain disease diagnosed on MRI
Authors: Guy Bethany , Freeman Paul , Khan Sam , Genain Marie‐Aude 
Published: October 2024

AbstractThe effect of midline shift identified on brain MRI on survival time in dogs with structural brain disease is relatively unknown. This retrospective single‐centered cohort study reviewed medical and imaging data of 77 dogs with structural brain lesions evident on MRI. Images were reviewed for the presence of midline shift, brain edema, foramen magnum herniation, and ventriculomegaly. Kaplan–Meier method and Cox regression analysis were undertaken to compare survival between dogs with and without midline shift. Midline shift was present in 40 of 77 (52%) dogs and absent in 37 of 77 (48%). Univariate analysis revealed that dogs with midline shift had a median survival time of 34.5 days (95% CI, 4–108 days) compared with 241 days (95% CI, 133,‐ days) in dogs without midline shift (hazard ratio = 2.67, 95% CI, 1.5–4.49). Multivariate Cox regression analysis revealed a hazard ratio of 3.6 (95% CI, 1.7–7.6; P‐value < .001) for dogs with midline shift. Shorter median survival times remained significant in all groups after segregation based on etiological diagnosis. The significantly shorter survival times observed herein for dogs with midline shifts, regardless of etiologic cause, provide further evidence that midline shift holds value as a negative prognostic factor in diagnostic imaging.
<p class="MDPI12title">Entomopathogenic fungi, Hirsutella thompsonii F.E. Fisher Safe Mycoacaricide for the Management of Coconut Mite, <span style="color: windowtext;">Aceria guerreronis</span><span style="color: windowtext;"> Keifer</span> in Thailand.
Authors: Jampameung Nattaya , Arikit Siwaret , Sanguansub Sunisa , Buttachon Suradet 
Published: October 2024

Aceria guerreronis Keifer (Acari: Eriophyidae) is a phytophagous mite that is one of the serious pests of coconut fruits worldwide. Chemical controls have been and continue to be the primary means of control. Because pesticide compatibility with natural enemies is a major concern for IPM systems. The aim of this study was to explore the contact toxicity and egg hatching inhibition of conidia suspension and crude ethyl acetate extract of Hirsutella thompsonii F.E. Fisher against adults and eggs of A. guerreronis Keifer. The results indicated that six isolates of H. thompsonii F.E. Fisher were evaluated for their pathogenicity against eggs and adults of the coconut mite (A. guerreronis Keifer), and H. thompsonii F.E. Fisher TBRC 6805 and TBRC 6933 resulted in the highest mortality (100%) on the 3rd day post-inoculation (DPI). The crude ethyl acetate extracts 5% (w/v) of H. thompsonii F.E. Fisher isolates TBRC-BCC 13865 showed the highest mortality to eggs (92%) and adults (98%) of A. guerreronis Keifer, and the LC50 value was 2.629% (eggs) and 1.704% (adults) after 72 h posttreatment. This study shows that H. thompsonii F.E. Fisher, a fungus well known for controlling biotic stress brought on by insect pests, can also have potential for the development of novel bioacaricides to control A. guerreronis Keifer in an integrated pest management program.
24-Hour Movement Behaviors of LGBTQA+ Young People: A Systematic Review
Authors: Fortnum Kathryn , Gomersall Sjaan R. , Ross Megan H. , Woodforde James , Thomas George , Wen Yu-Shu , Perales Francisco , Stylianou Michalis 
Published: October 2024

Background: The movement behaviors of LGBTQA+ young people, who encounter specific health, and other, challenges are not well understood. This systematic review examines the 24-hour movement behaviors of LGBTQA+ young people compared with population estimates of meeting the 24-hour movement guidelines. Methods: Seven electronic databases were searched from inception to January 2024. Observational studies published in English and reporting quantitative data for physical activity, sedentary behavior, or sleep duration for LGBTQA+ individuals <24 years old were included. Data were narratively synthesized for children/adolescents (<18 years) and young adults (18–24 years), guided by the Cochrane Synthesis Without Meta-analysis guidelines. Results: Fifty-six studies were included; 46 were of “fair” quality and 37 were conducted in the United States. Physical activity, sedentary behavior, and sleep outcomes were reported in 46, 11, and 14 studies, respectively. Ninety unique LGBTQA+ terms were identified. Based on outcomes reported in ≥1 study, and compared to population estimates, LGBTQA+ young people were less likely to meet aerobic physical activity and strength/resistance training guidelines. LGBTQA+ children/adolescents were less likely to meet the sleep guidelines. Young adults demonstrated similar adherence to sleep guidelines as population estimates. Conclusions: Overall, our results suggest that LGBTQA+ young people have suboptimal 24-hour movement behaviors; in some cases, worse than population estimates. Utilization of more robust measures of exposure and outcome variables is recommended, with a focus on sleep and sedentary behavior.
Can letrozole be repurposed for the treatment of visceral leishmaniasis?
Authors: Ribeiro Juliana Martins , Teixeira Eliane de Morais , Alves Líndicy Leidicy , Alves Érica Alessandra Rocha , Pascoal-Xavier Marcelo Antônio , Santi Ana Maria Murta , Oliveira Edward , Guimarães Pedro Pires Goulart , Teixeira-Carvalho Andrea , Murta Silvane Maria Fonseca , Peruhype-Magalhães Vanessa , Souza-Fagundes Elaine Maria 
Published: October 2024

ABSTRACT

Visceral leishmaniasis, caused by
Leishmania infantum
in New World countries, is the most serious and potentially fatal form of leishmaniasis, if left untreated. There are currently no effective prophylactic measures, and therapeutic options are limited. Therefore, we investigated whether the aromatase inhibitor letrozole (LET), which is already used to treat breast cancer, has an antileishmanial activity and/or immunomodulatory potential and therefore may be used to treat
L. infantum
infection. LET was active against
L. infantum
promastigote and amastigote life cycle stages in an i
n vitro
infection model using human THP-1 cell-derived macrophages. In human peripheral blood leukocytes
ex vivo
, LET reduced the internalized forms of
L. infantum
by classical monocytes and activated neutrophils. Concomitantly, LET stimulated the production of IL-12/TNF-α and decreased the production of IL-10/TGF-β by peripheral blood phagocytes, while in T and B cells, it promoted the production of TNF-α/IFN-γ and decreased that of IL-10. In a murine infection model, LET significantly reduced the parasite load in the liver after just 5 days and in the spleen after 15 days. During
in vivo
treatment with LET, the production of TNF-α/IFN-γ also increased. In addition, the proportion of developing granulomas decreased and that of mature granulomas increased in the liver, while there was no significant change in organ architecture in the spleen. Based on these data, repositioning of LET may be promising for the treatment of visceral leishmaniasis in humans.
Excellent anti-mildew effect of essential oil impregnation on sliced veneer plybamboo and its anti-mildew mechanism
Authors: Bao Qichao , Shan Yingying , Yang Fei , Zhu Jiawei , Du Chungui , Wang Yuting , Shao Yuran , Liu Chunlin , Chen Shiqin , Ran Ying , Yin Wenxiu 
Published: October 2024

ABSTRACT

Sliced bamboo veneer used as a high-end decoration material is a highly innovative material for the deep processing of bamboo. However, bamboo is rich in starch and small molecular soluble sugars, making it susceptible to mildew infection and limiting the wide application of sliced veneer plybamboo. Spice essential oils are considered green and safe antimildew agents, which are cheap and accessible. The natural phenolic substances in plant essential oil have a good inhibitory effect on bamboo mildew. In this study, three types of spice essential oils (clove essential oil, oregano essential oil, and fennel essential oil) were employed, and their antifungal activity against bamboo mildews was assessed using the Oxford cup method, scanning electron microscopy, transmission electron microscopy, and a micro pH meter. The results demonstrated that the diameters of the Inhibition Zone against four common bamboo mildews (AN
, Aspergillus niger;
TV
, Trichoderma viride;
PC,
Penicillium citrinum
; MM, Mixed Mildews) caused by clove essential oil were 25.68 mm, 23.22 mm, 30.68 mm, and 25.43 mm, respectively. As an explanation, clove essential oil can inhibit or eliminate mildew by damaging and disrupting the cell membrane of the bamboo mildew, leading to significant shrinkage, distortion, surface roughness, formation of holes, or partial structural cracks in the mildew’s mycelium. Additionally, it may interfere with and disrupt the pH balance of the intracellular and extracellular fluids within the cell. Furthermore, we also report that sliced veneer plybamboo impregnated with clove essential oil on each layer showed fine inhibition rates of 50%, 75%, 100%, and 25% against AN, TV, PC, and MM, respectively. This research underscores a sustainable approach to mildew prevention, crucial for advancing bamboo’s utilization in high-value furniture decor applications.

IMPORTANCE
Mildew growth in sliced veneer plybamboo poses a significant challenge, particularly in its use for high-end furniture and decor. Traditionally, chemical treatments have been the primary solution though they often raise environmental concerns. Essential oils, with their well-documented antimicrobial properties, have emerged as an important natural and eco-friendly alternative for preventing mildew. These oils inhibit mildew growth effectively while offering a sustainable, non-toxic solution that reduces harm to both the environment and human health. By leveraging essential oils, it becomes possible to extend the lifespan of bamboo products, making them more durable and suitable for broader applications in furniture and decor, all while addressing the ecological limitations of conventional mildew prevention methods.
Association between early pulmonary arterial pressure measurements and bronchopulmonary dysplasia or mortality in very preterm infants: a prospective cohort study
Authors: Akin Mustafa Senol , Kas Gökce , Aydin Emre , Cetinkaya Aslıhan Kose , Ece Ibrahim , Sari Fatma Nur , Alyamac Dizdar Evrim 
Published: October 2024

BackgroundPrematurity is a significant risk for bronchopulmonary dysplasia related pulmonary artery pressure.ObjectiveTo determine the association between pulmonary artery pressure in the early days of life and the development of bronchopulmonary dysplasia or mortality.MethodsThis prospective observational cohort study included infants born at <32 weeks and weighing <1500 g. Pulmonary artery pressure was measured between postnatal days 3 and 7. Pulmonary hypertension was defined as systolic pulmonary artery pressure ≥40 mm Hg or systolic pulmonary artery pressure/systolic blood pressure >0.5 (pulmonary hypertension criterion-1). Infants were categorised into pulmonary hypertension and non-pulmonary hypertension groups. The primary endpoint was bronchopulmonary dysplasia or mortality. Receiver operating characteristic analysis established a new threshold value for predicting bronchopulmonary dysplasia or mortality (pulmonary hypertension criterion-2). Infants were reanalysed according to new criteria.ResultsA total of 329 infants were included in this study. Moderate-to-severe pulmonary hypertension was identified in 24% (n=79) of the infants. The pulmonary hypertension group exhibited a significantly lower gestational age, lower birth weight and a higher incidence of small for gestational age. Systolic pulmonary artery pressure >25 mm Hg or systolic pulmonary artery pressure/systolic blood pressure >0.35 was defined as the pulmonary hypertension criterion-2. Logistic regression analysis identified pulmonary hypertension criterion-2 as an independent risk factor for moderate-to-severe bronchopulmonary dysplasia or mortality (OR 2.67, 95% CI 1.3 to 5.51, p<0.01).ConclusionPulmonary artery pressure exceeding 25 mm Hg in the early days of life may be considered a potential risk factor for bronchopulmonary dysplasia or mortality.
Tale of quadriceps and hamstring muscle strength after ACL reconstruction: a systematic review with longitudinal and multivariate meta-analysis
Authors: Girdwood Michael , Culvenor Adam G , Rio Ebonie K , Patterson Brooke E , Haberfield Melissa , Couch Jamon , Mentiplay Benjamin , Hedger Michael , Crossley Kay M 
Published: October 2024

ObjectiveThis study aimed to investigate how knee extensor and flexor strength change over time after anterior cruciate ligament reconstruction (ACLR).DesignSystematic review with longitudinal meta-analysis.Data sourcesMedline, Embase, CINAHL, Scopus, Cochrane CENTRAL and SPORTDiscus to 28 February 2023.Eligibility criteriaStudies of primary ACLR (n≥50), with mean participant age 18–40 years, reporting a quantitative measure of knee extensor or flexor strength were eligible. Muscle strength had to be reported for the ACL limb and compared with: (1) the contralateral limb (within-person); and/or (2) an uninjured control limb (between-person).ResultsWe included 232 studies of 34 220 participants. Knee extensor and flexor strength showed sharp initial improvement postoperatively before tailing off at approximately 12–18 months post surgery with minimal change thereafter. Knee extensor strength was reduced by more than 10% compared with the contralateral limb and approximately 20% compared with uninjured controls at 1 year for slow concentric, fast concentric and isometric contractions. Knee flexor strength showed smaller deficits but was still 5%–7% lower than the contralateral limb at 1 year for slow concentric, fast concentric and isometric contractions. Between-person comparisons showed larger deficits than within-person comparisons.ConclusionKnee extensor muscle strength is meaningfully reduced (>10%) at 1 year, with limited improvement after this time up to and beyond 5 years post surgery. Many people likely experience persistent and potentially long-term strength deficits after ACLR. Comparison within person (to the contralateral limb) likely underestimates strength deficits in contrast to uninjured controls.
Overdose as a complex contagion: modelling the community spread of overdose events following law enforcement efforts to disrupt the drug market
Authors: Humphrey Jamie L , Schwab Clyde , Richardson Nicholas J , Lambdin Barrot H , Kral Alex H , Ray Bradley 
Published: October 2024

Background
The opioid overdose mortality crisis in the USA is an ongoing public health epidemic. Ongoing law enforcement strategies to disrupt local unregulated drug markets can have an iatrogenic effect of increasing overdose by driving consumers towards new suppliers with unpredictable drug products of unknown potency.

Methods
Cross-sectional study using point-level information on law enforcement opioid-related drug seizures from property room data, opioid-related non-fatal overdose events from emergency medical services and block group-level social determinants of health data from multiple sources. Using an endemic-epidemic spatiotemporal regression model, we estimated the degree to which exposure to drug supply disruptions triggers future overdose events within small space-time distances in Indianapolis, Indiana.

Results
Neighbourhoods with more structural racism, economic deprivation or urban blight were associated with higher rates of non-fatal overdose. Exposure to an opioid-related drug seizure event had a significant and positive effect on the epidemic probability of non-fatal overdose. An opioid seizure that occurred within 250 m and 3 days, 250 m and 7 days, and 250 m and 14 days of an overdose event increased the risk of a new non-fatal overdose by 2.62 (rate ratio (RR)=2.62, 95% CI 1.87 to 3.67), 2.17 (RR=2.17, 95% CI 1.87 to 2.59) and 1.83 (RR=1.83, 95% CI 1.66 to 2.02), respectively. Similar spatiotemporal patterns were observed in a smaller spatial bandwidth.

Conclusions
Results demonstrated that overdoses exhibit a community spread process, which is exacerbated following law enforcement strategies to disrupt the unregulated drug market. We discuss decriminalisation and increasing resources that promote safer drug use to combat this public health crisis.