bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of curculigoside on osteogenic differentiation and orthodontic tooth movement in osteoporosis under mechanical stretch stress
Authors: Liu Xinyu , Jiang Huang , Wang Huimin , Li Yutong , Hu Min 
Published: September 2024

Abstract

Curculigoside (CCG), a natural glycoside compound extracted from the traditional Chinese medicinal herb Curculigo orchioides, is known for its osteogenic differentiation-promoting effects. In this study, we investigated the anti-osteoporotic effects of CCG under mechanical stress. Establishing an osteoporotic orthodontic force cell model, we found that CCG treatment could promote osteogenic differentiation in MC3T3-E1 cells and enhance the expression of osteogenic differentiation factors such as COL1, BMP2, ALP, and RUNX2. Furthermore, we applied CCG to osteoporotic rats undergoing orthodontic tooth movement (OTM) to observe its in vivo anti-osteoporotic activity. Through the detection of serum ALP levels, X-ray of femur, and Micro-CT of maxillary, we found that CCG could alleviate the reduction in bone density caused by osteoporosis (OP) and decrease the loss of alveolar bone mass during OTM. In summary, CCG can promote osteogenic differentiation of MC3T3-E1 cells under mechanical stress, and it is beneficial for bone formation during OTM in OP.
Integrative Computational Approaches to Assess Anti-Influenza Activity in Caesalpinia mimosoides Lamk Hydroethanolic Extract
Authors: Klamrak Anuwatchakij , Rahman Shaikh Shahinur , Nopkuesuk Napapuch , Nabnueangsap Jaran , Narkpuk Jaraspim , Janpan Piyapon , Saengkun Yutthakan , Soonkum Thananya , Sribu Supawadee , Teeravechyan Samaporn , Sitthiwong Poramet , Jangpromma Nisachon , Kulchat Sirinan , Choowongkomon Kiattawee , Patramanon Rina , Chaveerach Arunrat , Daduang Jureerut , Daduang Sakda 
Published: September 2024

Abstract

In a recent study, we used chemical analysis to show that the Caesalpinia mimosoides aqueous extract, which contains a high concentration of simple phenolics, has strong anti-influenza activity. We determined through molecular docking methods that its potential target inhibitor is the neuraminidase. Therefore, our study objectives were to evaluate whether the aqueous-ethanol extract (30% v/v) of this plant species exhibits greater antiviral activity than the aqueous plant extract. The C. mimosoides hydroethanolic extract exhibited potent antioxidant activity in the DPPH assay, with an IC50 value of 15.01 µg/mL, comparable to authentic quercetin (IC50 = 12.72 µg/mL) and approximately 4.91 times greater than standard gallic acid (IC50 = 3.06 µg/mL). Through untargeted metabolomic analyses (UPLC-ESI(±)-QTOF-MS/MS) and subsequent stepwise computational metabolomics analyses, we identified the extract as primarily containing simple phenolics (e.g., gallic acid, ellagic acid, shikimic acid, and chlorogenic acid), flavonoid derivatives (e.g., quercetin, taxifolin, myricitrin, and afzelin), and other bioactive components, including dicarboxylic acids and germacrone. The polyphenol-rich extract showed strong anti-influenza activity, with an IC50 of 2.33 µg/mL against the influenza A/PR/8/34 virus and no cytotoxic effects, as indicated by a CC50 greater than 50 µg/mL. This represents an approximately 3.35-fold increase in effectiveness compared to its corresponding aqueous extract (IC50 = 7.81 µg/mL). Furthermore, the extract demonstrated no hemolytic activity, even at a maximum concentration of 2,000 µg/mL, suggesting its potential as a safe antiviral agent. Molecular docking analyses revealed that the identified phytochemicals can simultaneously interact with the "drug-target binding sites" of neuraminidase (NA) and the PB2 subunit of influenza RNA polymerase, indicating their potential polypharmacological effects. Given the consistent efficacy of polyphenols in targeting various viral machinery proteins, we are currently investigating the anti-SARS-CoV-2 properties of our established plant extract to expand its potential applications.
Quantitative Study on Hepatic Genotoxicity of Neodymium and Its Molecular Mechanisms Based on Benchmark Dose Method
Authors: Wang Ning , Chang Xiu-li , Leng Jing , Cheng Xue-qing , Ding Yu , Xu Jing , Yang Zhengli , Zhang Hui-min , Sun Jing-qiu , Hong Xin-yu , Jia Xu-dong 
Published: September 2024

Abstract

Neodymium has been shown to induce genotoxicity in mice, but the molecular mechanisms behind this effect are not fully understood. To clarify the genotoxic effects of intragastric neodymium nitrate (Nd(NO3)3) administration over 28 consecutive days, we assessed the percentage of tail DNA in mouse hepatocytes using the alkaline comet assay, genetic toxicological biomarkers, and the expression levels of genes and proteins related to the p53 pathway in the mouse liver. Our results indicated significant accumulation of Nd(NO3)3 in the livers and kidneys of mice, resulting in micronuclei formation and DNA double-strand breaks, as indicated by comet and γ-H2AX assays, as well as DNA damage in hepatocytes. Nd(NO3)3 significantly increased the percentage of tail DNA in hepatocytes as measured by the alkaline comet assay and upregulated the expression of p53 pathway-related molecules, including ATM, Wip1, ATR, Chk2, MDM2, p53, p21, and NF-kB, at both the transcriptional and translational levels. This treatment effectively triggered the production of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and γ-H2AX in liver tissue. These findings suggest that Nd(NO3)3 induces hepatic genotoxicity and injury in mice, and modulates the expression of genes associated with DNA damage response, carcinogenesis, and inflammatory processes.
In-Situ Bragg Coherent Diffraction Imaging ofDomain Wall Motion in Multiferroic DyMnO3 Nanocrystal
Authors: Najeeb Mansoor A. , Morrison Robbie , Mokhtar Ahmed H. , Porter Daniel G. , Lichtenberg Frank , Bombardi Alessandro , Spaldin Nicola A. , Newton Marcus C. 
Published: September 2024

Abstract

Multiferroic materials which exhibit interacting and coexisting properties, like ferroelectricity and ferromagnetism, possess significant potential in the development of novel technologies that can be controlled through the application of external fields. They also exhibit varying regions of polarity, known as domains,with the interfaces that separate the domains referred to as domain walls. In this study, using three-dimensional Bragg Coherent Diffractive Imaging (BCDI), we investigate the dynamics of multiferroic domain wall motion in a single hexagonal dysprosium manganite (h-DyMnO3 ) nanocrystal under varying applied electricfield. Our analysis reveals the dynamics of the ferroelectric polarisation where switching of the direction was achieved with an applied field of less than 0.3 V.
Study on erosion characteristics of gas-liquid-solid three-phase flow on drilling four-way
Authors: Liu Pu , zhang ruiqi , Wang Zhiping , Tan Ruifeng , Li Kaixuan 
Published: September 2024

Abstract

In the process of drilling oil and gas Wells, due to the complexity of the formation fluid pressure system and the subjective and objective factors in drilling operations, the original pressure balance in the wellbore may be destroyed, resulting in the formation fluid entering the well and causing the overflow phenomenon. When studying the erosion wear of drilling four-pass, most of them focus on the wear effect caused by two-phase flow, while ignoring the impact caused by gas-liquid-solid three-phase flow. Aiming at the erosion wear problem of four-pass pipeline during the well killing process when gas encroachment occurs, based on the DPM erosion prediction model, FLUENT software is used to numerically simulate the erosion wear rule of four passes when gas encroachment occurs, and the change rule of the erosion wear position and maximum erosion rate of drilling four passes caused by different particle size and flow rate of cuttings is analyzed. The results show that the erosion damage is mainly concentrated in the bypass inlet intersecting line and bypass channel. The gas-liquid-solid three-phase flow caused by gas invasion will aggravate the erosion wear. When the mass flow rate of cuttings is constant, the maximum erosion rate at the intersecting line also increases with the increase of particle size. Only the mass flow rate of cuttings is changed, and the maximum erosion rate increases with the mass flow rate increasing.
A multi-fidelity transfer learning strategy for surface deformation measurement of large reflector antennas
Authors: Zhang Zihan , Ye Qian , Wang Na , Meng Guoxiang 
Published: September 2024

Abstract

As the observation frequency of large-aperture antennas increases, the requirements for measuring main reflector deformation have become more stringent. Recently, the rapid development of deep learning has led to its application in antenna deformation prediction. However, achieving high accuracy requires a large number of high-fidelity deformation samples, which is often challenging to obtain. To address these problems, this paper establishes a high-accuracy antenna surface deformation measurement model based on a multi-fidelity transfer learning neural network (MF-TLNN). Firstly, a low-fidelity surrogate model is constructed using a large number of simulation deformation samples to ensure its robustness. Secondly, the MF-TLNN structure is designed and trained using a small number of high-fidelity samples obtained from actual measurements of the main reflector deformation. Thirdly, a Zernike correction module is utilized to provide additional constraints and ensure the stability of the results. Experimental results show that the proposed method can closely approximate radio holography measurements in terms of accuracy and is almost real-time in terms of speed.
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
Authors: Yuan Gankun , Yang Ruyue , Wen Wenjing , Wei Zhaoyi , Song Meiru , Zhang Lingyang , Hou Kun , Liang Gaofeng 
Published: September 2024

Abstract

Background Activating immunogenic cell death (ICD) represents a promising therapeutic strategy for tumor immunotherapy. However, photodynamic therapy (PDT)-mediated ICD effects are severely limited due to the extremely short half-life and limited diffusion radius of reactive oxygen species (ROS) hinder effective endoplasmic reticulum (ER) stress induction. In addition, targeted therapy of triple-negative breast cancer (TNBC) remain hugely challenging due to the lack of expression of multiple receptors.
Results Herein, we synthesized a hierarchical targeting and controllable intelligent nanodelivery material Da-CD@CET@CQ, loaded with highly efficient ER-targeted photosensitizers CET and autophagy inhibitor chloroquine (CQ). Excitingly, Da-CD@CET@CQ NPs can selectively target TNBC tumor cells and the CET was effectively released in the tumor microenvironment, enabling local accumulation of photosensitizers in the ER and in situ ROS production, which causing stronger ER stress and amplifying the ICD effect, further increasing the immune suppression of tumor growth. More importantly, CQ released by Da-CD@CET@CQ NPs can inhibit autophagy to destroy damaged cell repair, and further enhance the anti-tumor ability of PDT.
Conclusions Our findings indicate that we reported a novel strategy of photosensitizing ICD inducer to amplify ICD effects and combination with autophagy inhibition, which provides a meaningful guideline for targeted PDT synergistic immunotherapy of TNBC in the future.
An Inverter based OTA with Novel Tunable Pseudoresistor and its Application in Arrhythmia Detection using Machine Learning Algorithms
Authors: FARSHORI MOHAMMAD , NIZAMUDDIN M , HASHMI FARAZ , ASLAM FAIZ , ZULQARNAIN MOHAMMAD 
Published: September 2024

Abstract

In this work, inverter based operational trans-conductance amplifier (OTA) is designed using different configurations of FinFET and CNTFET. Performance parameters (gain, power, bandwidth, and output resistance) of the OTA have been analyzed with variation of CNT parameters. In addition, inverter based OTA is used as a core amplifier in chopper stabilized amplifier. Three different novel configurations of tunable pseudoresistor are used as feedback resistor in chopper stabilized amplifier and their impact on OTA design has been observed. Also, a machine learning based model is designed for arrhythmia diagnosis. The performance of the designed model is evaluated using training accuracy and training loss for various algorithms. Confusion matrix is also used to show the performance of various algorithms.
Clinical and basic longitudinal analyses of root canal therapy
Authors: Júnior Wilson Bambirra , Guimarães Victor Hugo Dantas , Maia Lucas Moreira , de Brito Luciana Carla Neves , Vieira Leda Quercia , Guimarães André Luiz Sena , Marquiore Larissa Fassarela , Sobrinho Antônio Paulino Ribeiro , Tavares Warley Luciano Fonseca 
Published: September 2024

Abstract

Objective
To quantitatively assess the gene expression of microbial rRNA 16S and fragments of the Internal Transcribed Spacer (ITS) region of fungi within infected root canals.
Materials and methods
Twenty-two individuals undergoing endodontic treatment were divided into two groups: 11 with infected teeth and 11 without infection. #20 paper points were inserted into the pulp cavity at various time points (T0, T1, T2, and T3) during the cleaning and shaping procedure. Real-time PCR was utilized to analyze the gene expression of microbial 16S rRNA and fragments of the ITS region of the gDNA of Candida species, as well as the expressions of cytokine and chemokine genes.
Results
The expression levels of 16S mRNA, IL-1β, and IL-17 decreased after cleaning and shaping procedures, while an initial increase in Candida abundance was noted in infected teeth. TNF-α expression significantly increased in both groups. No significant differences were observed in the expression of IFN-γ, IL-10, CCL-2, CCL-5, ITGAL, and ITGA5. Over time, expressions of ITGA1 and ITGA4 in the experimental group notably decreased. The mRNA expression of the macrophage cell marker (CD64) showed no significant differences between the groups. In contrast, mRNA expression in fibroblasts (S100A4) significantly increased 15 days post-treatment.
Conclusion
A correlation was found between bacterial load, yeast abundance in root canal systems, and mRNA expression of cytokines and chemokines.
Clinical Relevance
The undertaking of fundamental scientific research is indispensable in comprehending the dynamics of molecules and microorganisms, enabling the assessment of factors that directly or indirectly impact the clinical framework.
Asymptotic normality for the estimators in heteroscedastic semiparametric EV models with α-mixing errors
Authors: Wang Xuejun , Xi Mengmei , Huang Haiwu , Wang Rui 
Published: September 2024

Abstract

In this paper, the heteroscedastic semi-parametric errors-in-variables (EV) model, yi = ξiβ + g(ti) + ϵi, xi = ξi + μi, is considered, where ϵi = σiei, σ2 i = f(ui), β is an unknown parameter to be estimated, g(·) and f(·) are unknown functions to be estimated. The asymptotic normalities for the estimators of β, g(·) and f(·) are presented under α-mixing random errors in either the errors are homoscedastic or heteroscedastic. Simulation studies are concerned to verify the finite sample behavior of the estimators.
Mathematical Subject Classification: 62E20; 62F12; 62G20
Integrating self-efficacy and career anchor: a multi-dimensional model to enhance vocational college students' employment competitiveness
Authors: shuangshuang liu 
Published: September 2024

Abstract

With With the growth of the digital economy and the advancement of China's “Made in 2025” initiative, people pay more and more attention to the employment competitiveness of graduates from vocational colleges. Based on career anchor theory and self-efficacy theory, this study uses a partial least square structural equation model to analyze the sample data. It discusses the influence mechanism of cross-professional learning, career development and planning, and career network expansion on employment competitiveness. The results show that these factors significantly improve students' employment competitiveness, while self-efficacy and employment matching play an important mediating role. This study provides valuable insights for governments in formulating education policies, emphasizes the importance of supporting cross-disciplinary learning and providing systematic and personalized career development and planning guidance, and makes specific recommendations to strengthen career guidance and services. This study innovatively integrates self-efficacy theory and career anchor theory to build a brand new theoretical framework. Such cross-theoretical integration not only deepens the understanding of the psychological mechanism of individual career development, but also provides a new research path and perspective for subsequent research.
Effectiveness of Yoga Pranayama in Reducing Anxiety Levels Among Caregiver of Children With Mental Disorders in Denpasar
Authors: Putra Surya Pradnyana , Ardani I Gusti Ayu Indah , Sutrisna I Putu Belly , Putra I Wayan Gede Artawan Eka 
Published: September 2024

Abstract

Background: Caregivers of children with mental disorders often face significant psychological challenges, including elevated anxiety levels. This study aimed to assess the effectiveness of yoga pranayama as a non-pharmacological intervention in reducing anxiety among these caregivers in Denpasar.
Method: The research employed a pre-experimental design with a one-group pre-post-test approach involving 36 caregivers recruited from the Disability Service Center in Denpasar City. The intervention consisted of 30-minute yoga pranayama sessions conducted once a week for four consecutive weeks. Anxiety levels were measured using the Hamilton Anxiety Rating Scale (HARS) before and after the intervention period.
Results: The results revealed a significant decrease in HARS scores from pre-test (29.5 ± 8.56) to post-test (6.42 ± 3.65), with paired t-test analysis showing a mean difference of 23.083 points (95% CI: 20.205 - 25.962, p < 0.001).
Conclusion: These findings demonstrate that yoga pranayama is an effective strategy for reducing anxiety levels among caregivers of children with mental disorders in Denpasar. The substantial and statistically significant reduction in anxiety scores suggests that integrating yoga pranayama into support programs for caregivers could be a valuable approach to improving their mental well-being. This study contributes to the growing evidence supporting mind-body interventions in caregiversupport. It highlights the potential of yoga pranayama as an accessible and beneficial practice for this population.
Green Synthesis of Silver Nanoparticles using Alkanna Tinctoria Aqueous Leaf Extract and its Antibacterial and Antioxidant Activity Against Cancer Cell Lines
Authors: Alotaibi Rajeh , Alshammari Riyadh H. , Almadhhi Sultan , Alobaidi Ahmed , Alshammari Saad , Rady Ahmad 
Published: September 2024

Abstract

Nanotechnology plays a pivotal role in advancing materials science, particularly through the development of nanoparticles, which are integral in a variety of industrial applications. This study focuses on silver nanoparticles (AgNPs), which are highly valued in multiple sectors due to their unique physical, chemical, and biological properties. We explore the green synthesis of AgNPs using the aqueous leaf extract of “Alkanna tinctorial”. This method not only ensures a sustainable and eco-friendly approach but also facilitates the production of nanoparticles with controlled sizes and shapes, which are crucial for their efficacy and application versatility. The synthesized AgNPs were characterized using advanced techniques including X-ray Diffraction (XRD), UV-visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), and Transmission Electron Microscopy (TEM). These characterizations confirm the successful synthesis and desired physicochemical properties of the AgNPs. The research highlights the broad applications of these AgNPs, demonstrating significant antimicrobial properties and potential anticancer activities. The nanoparticles showed a notable efficacy against a range of bacterial strains and exhibited promising anticancer properties in vitro, particularly tested against the HepG2 cell line. The AgNPs induce apoptosis and inhibit cellular proliferation, showcasing their potential as therapeutic agents in medical applications. This study substantiates the potential of biogenically synthesized AgNPs to serve as a safer, more efficient alternative to conventional nanoparticles, offering significant advantages in healthcare and beyond, owing to their tailored functionalities and reduced toxicity. The findings promote further exploration and development of green synthesis methods in nanoparticle production, ensuring sustainability and broad applicability in modern technology and medicine.
Laser methane emission monitoring based on harmonic ratio feature and estimation of absorption pulse parameters
Authors: Nguyen Bach , Baryskievic Anatol , Baryskievic Illa , Tsviatkou Viktar 
Published: September 2024

Abstract

Monitoring of methane from oil and natural gas operations is essential to mitigate environmental risks and promote sustainable practices. The method is an effective tool to detect methane presence and compute methane concentration under condition of low signal to noise ratio. The proposed adaptive time synchronous moving average, multiband decomposition and pulse signal fusion of laser signal allow to reduce measurement noise. The harmonic ratio features and time-amplitude features improve taking into consideration the properties of the absorption signal and pulse allow to improve the methane estimation accuracy. Experimental results show that the methane detection accuracy and classification accuracy are equal to 100% and 86.5%, respectively. The estimation accuracy of methane concentration in terms of R2 value equal to 0.995.
How universal adhesive systems with nanoencapsulated flavonoids improve long-term bonding to caries-affected dentin
Authors: Falconi-Páez Camila , Núñez Alejandra , Navarro Tobia Gastón , González-Vaca Claudia Carolina , Campos Lucas Arrais , Dávila-Sánchez Andrés , Arrais Cesar Augusto Galvão 
Published: September 2024

Abstract

Objectives
To evaluate the effect of nano-encapsulated flavonoids in universal adhesives on resin-dentin bond-strength (µTBS) and nanoleakage (NL) on artificial caries-affected dentin (CAD).
Materials and Methods
Artificial CAD was created on the occlusal dentin surfaces of 60 human third molars by a microbiological assay. Nanoencapsulated quercetin (Q) and naringin (N) were incorporated into Prime&Bond Universal (PBU; Dentsply-Sirona) and Single Bond Universal (SBU, 3M ESPE). The adhesive systems with and without (control) flavonoids were applied to the CAD surface, which was validated by Knoop microhardness (KNH), and a 4-mm resin composite block (TPH Spectrum, Dentsply Sirona) was built up and light-cured. Specimens were sectioned into resin-dentin sticks and tested in tension in a universal testing machine. µTBS and NL tests were performed after 24-h and 6-month water storage (WS). The HL was subjected to micro-raman analysis to detect N and Q. µTBS and NL data were analyzed using a non-parametric three-way repeated measures ANOVA test followed by Bonferroni's test (α = 5%). KNH data was analyzed using a paired Student´s t test.
Results
CAD exhibited significantly lower KNH values than sound dentin. N and Q nanocapsules increased µTBS and reduced NL values after WS. At 24-h, PBU group showed higher NL values than SBU group, and the values decreased after WS. Q and N were detected within the HL.
Conclusions
Incorporating nanoencapsulated flavonoids may improve longevity of universal bonding systems applied to CAD.
Clinical Relevance:
Adhesives restorations with therapeutical compounds might be an option to create stable bonding over time.