bluuebunny/crossref_metadata_2025_split huggingface dataset:
OsCERK1 plays a crucial role in the lipopolysaccharide‐induced immune response of rice
Authors: Desaki Yoshitake , Kouzai Yusuke , Ninomiya Yusuke , Iwase Ryosuke , Shimizu Yumi , Seko Keito , Molinaro Antonio , Minami Eiichi , Shibuya Naoto , Kaku Hanae , Nishizawa Yoko 
Published: November 2017

Summary

Plant cell surface receptor‐like kinases (RLKs) mediate the signals from microbe‐associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram‐negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co‐receptors are unknown except for LORE, a bulb‐type lectin S‐domain RLK (B‐lectin SD1‐RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP. Here, we analyzed the relevance of OsCERK1 to LPS perception in rice.

Using OsCERK1‐knockout mutants (oscerk1), we evaluated hydrogen peroxide (H2O2) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B‐lectin SD1‐RLK genes in rice and for all LysM‐protein genes in Arabidopsis.

Compared with wild‐type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B‐lectin SD1‐RLK genes and Arabidopsis lines mutated in the LysM‐protein genes responded normally to LPS.

From these results, we conclude that OsCERK1 is an LPS receptor/co‐receptor and that the LPS perception systems of rice and Arabidopsis are significantly different.
Evolution on the bright side of life: microorganisms and the evolution of mutualism
Authors: Hillesland Kristina Linnea 
Published: November 2017

AbstractMutualistic interactions, where two interacting species have a net beneficial effect on each other's fitness, play a crucial role in the survival and evolution of many species. Despite substantial empirical and theoretical work in past decades, the impact of these interactions on natural selection is not fully understood. In addition, mutualisms between microorganisms have been largely ignored, even though they are ecologically important and can be used as tools to bridge the gap between theory and empirical work. Here, I describe two problems with our current understanding of natural selection in mutualism and highlight the properties of microbial mutualisms that could help solve them. One problem is that bias and methodological problems have limited our understanding of the variety of mechanisms by which species may adapt to mutualism. Another problem is that it is rare for experiments testing coevolution in mutualism to address whether each species has adapted to evolutionary changes in its partner. These problems can be addressed with genome resequencing and time‐shift experiments, techniques that are easier to perform in microorganisms. In addition, microbial mutualisms may inspire novel insights and hypotheses about natural selection in mutualism.
Leadless pacemaker extraction from a single‐center perspective
Authors: González Villegas Elkin , Al Razzo Omar , Silvestre García Jorge , Mesa García José 
Published: November 2017

AbstractBackgroundLeadless pacemaker can be considered as a technical revolution in cardiac pacing devices, with clear advantages over conventional pacemakers in overcoming all lead‐related complications. However, the management of these devices once they reach the end of life (EOL) of the battery is still controversial.In the next years, there will be an increase in the need to define a clear strategy in the management of leadless PM once they reach their EOL. Safe extraction of these devices will define in a great manner this strategyMethodsWe performed the extraction of three functioning Nanostim leadless pacemaker prophylactically in two females and one male patients as part of the Nanostim battery depletion field action recommendation. All patients had a prior transesophageal 3D echocardiography to determine the device intracardiac mobility and the extent of possible endothelialization. For the extractions, we used the Nanostim Retrieval Catheter S1RSIN (St. Jude Medical, St. Paul, MN, USA), which is a proprietary catheter provided by the manufacturing company based on a lasso.ResultsComplete extraction of the devices was achieved in all patients using a relatively short fluoroscopic time (16, 19, and 12 minutes).ConclusionsThe extraction of leadless pacemakers can be considered as a safe and feasible procedure using the tools provided by the manufacturer and designed for the extraction. However, a very low threshold must be maintained to avoid any risk to the patients. Our extraction time ranges are between 983 and 1,070 days, nevertheless it is necessary to gather more long‐term data to assess the feasibility and safety of these procedures.
Mycorrhizal tomato plants fine tunes the growth‐defence balance upon N depleted root environments
Authors: Sánchez‐Bel P. , Sanmartín N. , Pastor V. , Mateu D. , Cerezo M. , Vidal‐Albalat A. , Pastor‐Fernández J. , Pozo M.J. , Flors V. 
Published: November 2017

AbstractIn low nutritive environments, the uptake of N by arbuscular mycorrhizal (AM) fungi may confer competitive advantages for the host. The present study aims to understand how mycorrhizal tomato plants perceive and then prepare for an N depletion in the root environment. Plants colonized by Rhizophagus irregularis displayed improved responses to a lack of N than nonmycorrhizal (NM) plants. These responses were accomplished by a complex metabolic and transcriptional rearrangement that mostly affected the gibberellic acid and jasmonic acid pathways involving DELLA and JAZ1 genes, which were responsive to changes in the C/N imbalance of the plant. N starved mycorrhizal plants showed lower C/N equilibrium in the shoots than starved NM plants and concomitantly a downregulation of the JAZ1 repressor and the increased expression of the DELLA gene, which translated into a more active oxylipin pathway in mycorrhizal plants. In addition, the results support a priorization in AM plants of stress responses over growth. Therefore, these plants were better prepared for an expected stress. Furthermore, most metabolites that were severely reduced in NM plants following the N depletion remained unaltered in starved AM plants compared with those normally fertilized, suggesting that the symbiosis buffered the stress, improving plant development in a stressed environment.
Racial realism I: Are biological races real?
Authors: Spencer Quayshawn 
Published: November 2017

AbstractIn this article, I discuss and critique how metaphysicians of race have conceived of and defended racial realism according to how biologists use “race”. I start by defining “racial realism” in the broadest accepted way in the metaphysics of race. Next, I summarize a representative sample of recent attempts from metaphysicians of race and biologists to defend racial realism and the main criticisms against each attempt. I discuss how metaphysicians of race have defended racial realism according to how ordinary people use “race” in Part II.
The “Just Too Different” Objection to Normative Naturalism
Authors: Paakkunainen Hille 
Published: November 2017

AbstractConsider normative properties and facts, such as facts consisting in something's being what you ought to do, or the property of being morally wrong. Normative naturalism is the view that normative properties and facts such as these exist, and that they are natural properties and facts. Some suspect, however, that normativity is incompatible with a wholly naturalistic worldview: that the normative couldn't be natural because it's somehow “just too different” from the natural. I critically examine recent forms of this “just too different” objection to normative naturalism, with a view to making the debate accessible to a general philosophical audience.
Comparison of peri‐implant and periodontal marginal soft tissues in health and disease
Authors: Ivanovski Saso , Lee Ryan 
Published: November 2017

AbstractThe integrity of the peri‐implant soft‐tissue seal is crucial for maintaining peri‐implant tissue health. Whilst the transmucosal component of the restored implant shares some common features with teeth, namely the presence of a junctional epithelium and a connective tissue component, there are some important differences. A key difference is the nature of the relationship of the connective tissue with the implant surface, whereby there is ‘adaptation’ of collagen fibers in a parallel orientation in relation to the implant, but insertion of fiber attachment perpendicularly into cementum in the case of teeth. This, combined with reduced cellularity and vascularity in the peri‐implant connective tissue, may make implants more susceptible to disease initiation and progression. Furthermore, the presence of a subgingival connection between the implant and the abutment/restoration poses some specific challenges, and maintaining the integrity of this connection is important in preserving peri‐implant tissue health. Implant design features, such as the nature of the connection between the implant and the abutment, as well as the surface characteristics of the abutment and implants, may influence the maintenance of the integrity of soft tissue around implants. Iatrogenic factors, such as incorrect seating of the abutment and/or the restoration, and the presence of residual subgingival cement, will lead to loss of soft‐tissue integrity and hence predispose to peri‐implant disease.
Comparative Analysis of EGR and Air Dilution in Spark-Ignited Natural Gas Engines
Authors: Glewen William , Hoops Chris , Hiltner Joel , Flory Michael 
Published: November 2017

Industrial natural gas engines are used in a wide range of applications, each with unique requirements in terms of power density, initial cost, thermal efficiency, and other factors. As a result of these requirements, distinct engine designs have evolved to serve various applications. Heavy-duty spark-ignited engines can generally be divided into two broad categories based on their charge characteristics and method of emissions control.
Stoichiometric engines are widely used in applications where first cost, absolute emissions and relative engine simplicity are more important than fuel consumption. In most of the developed world, stoichiometric engines are equipped with a three-way catalyst to control emissions of nitrogen oxides (NOx) as well as products of incomplete combustion and raw unburned fuel.
Dilution of the charge mixture with excess air reduces the peak combustion gas temperature and associated heat rejection. As a result, lean burn engines are generally able to achieve higher efficiency and power density without inducing excessive component temperatures or end gas knock. NOx formation is mitigated by the reduced gas temperatures, such that most regulatory standards can currently be met in-cylinder. Significant obstacles exist to meeting more stringent future emissions regulations in this manner, however.
Another possible strategy is to dilute the charge mixture with recirculated exhaust gas. This offers similar benefits as air dilution while maintaining the ability to use a three-way catalyst for emissions after-treatment. While similar principles apply in either case, the choice of diluent can have a significant impact on knock resistance, emissions formation, thermal efficiency, and other parameters of importance to engine developers and operators.
This work aimed to examine the unique characteristics of EGR and air dilution from a thermodynamic and combustion perspective. A combination of cycle simulation tools and experimental data from a single-cylinder test engine was applied to demonstrate the impact of diluent properties on a fundamental level, and to illustrate departures from idealized behavior and practical considerations specific to the development of combustion systems for spark-ignited natural gas engines.
Experimental Study of Flame Stretch Under Engine-Like Conditions
Authors: Afkhami Behdad , Wang Yanyu , Miers Scott A. , Naber Jeffrey D. 
Published: November 2017

Since fossil fuels will remain the main source of energy for power generation and transportation in next decades, their combustion processes remain an important concern for the foreseeable future. For liquid or gaseous fuels, flame velocity that propagates normal to itself and relative to the flow into the unburned mixture is one of the most important quantities to study. In a non-uniform flow, a curved flame front area changes continually which is known as flame stretch. The concept becomes more important when it is realized that the stretch affects the turbulent flame speed.
The current research empirically studies flame stretch under engine-like conditions since there has not been enough experimental studies in this area. For this reason, a one-cylinder, direct-injection, spark-ignition, naturally-aspirated optical engine was utilized to image the flame propagation process inside an internal combustion engine cylinder on the tumble plane. The flame front was found by processing high speed images which were taken from the flame inside the cylinder.
Flame front propagation analysis showed that after the flame kernel was developed, during flame propagation period, the stretch rate decreased until the flame front touches the piston surface. This trend was common among stoichiometric, lean, and rich mixtures. In addition, the fuel-air mixture with λ = 0.85 showed lower stretch rate compared to stoichiometric or lean mixture with λ = 1.2. However, based on previous studies, further enrichment may result in the flame stretch rate become greater than that of the stretch rates for stoichiometric or lean mixtures. Also, comparing the stretch rate at two different engine speeds revealed that as the speed increased the stretch rate also increased; especially during the early flame development period. Therefore, according to previous studies which discussed flame stretch as a mechanism for flame extinguishment, the probability of the flame extinction is higher when the engine speed is higher.
A Computational Investigation of Fuel Chemical and Physical Properties Effects on Gasoline Compression Ignition in a Heavy-Duty Diesel Engine
Authors: Zhang Yu , Voice Alexander , Pei Yuanjiang , Traver Michael , Cleary David 
Published: November 2017

Gasoline compression ignition (GCI) offers the potential to reduce criteria pollutants while achieving high fuel efficiency in heavy-duty diesel engines. This study aims to investigate the fuel chemical and physical properties effects on GCI operation in a heavy-duty diesel engine through closed-cycle, 3-D computational fluid dynamics (CFD) combustion simulations, investigating both mixing-controlled combustion (MCC) at 18.9 compression ratio (CR) and partially premixed combustion (PPC) at 17.3 CR.
For this work, fuel chemical properties were studied in terms of the primary reference fuel (PRF) number (0–91) and the octane sensitivity (0–6) while using a fixed fuel physical surrogate. For the fuel physical properties effects investigation, PRF70 was used as the gas-phase chemical surrogate. Six physical properties were individually perturbed, varying from the gasoline to the diesel range.
Combustion simulations were carried out at 1375 RPM and 10 bar brake mean effective pressure (BMEP). Reducing fuel reactivity (or increasing PRF number) was found to influence ignition delay time (IDT) more significantly for PPC than for MCC due to the lower charge temperature and higher EGR rate involved in the PPC mode. 0-D IDT calculations suggested that the fuel reactivity impact on IDT diminished with an increase in temperature. Moreover, higher reactivity gasolines exhibited stronger negative coefficient (NTC) behavior and their IDTs showed less sensitivity to temperature change. When exploring the octane sensitivity effect, ignition was found to occur in temperature conditions more relevant to the MON test. Therefore, increasing octane sensitivity (reducing MON) led to higher reactivity and shorter ignition delay.
Under both MCC (TIVC: 385K) and PPC (TIVC: 353K), all six physical properties showed little meaningful impact on global combustion behavior, NOx and fuel efficiency. Among the physical properties investigated, only density showed a notable effect on soot emissions. Increasing density resulted in higher soot due to deteriorated air entrainment into the spray and the slower fuel-air mixing process. When further reducing the IVC temperature from 353K to 303K under PPC, the spray vaporization and fuel-air mixing were markedly slowed. Consequently, increasing the liquid fuel density created a more pronounced presence of fuel-rich and higher reactivity regions, thereby leading to an earlier onset of hot ignition and higher soot.
Physiologically based cord clamping stabilises cardiac output and reduces cerebrovascular injury in asphyxiated near-term lambs
Authors: Polglase Graeme R , Blank Douglas A , Barton Samantha K , Miller Suzanne L , Stojanovska Vanesa , Kluckow Martin , Gill Andrew W , LaRosa Domenic , te Pas Arjan B , Hooper Stuart B 
Published: November 2017

BackgroundPhysiologically based cord clamping (PBCC) has advantages over immediate cord clamping (ICC) during preterm delivery, but its efficacy in asphyxiated infants is not known. We investigated the physiology of PBCC following perinatal asphyxia in near-term lambs.MethodsNear-term sheep fetuses (139±2 (SD) days’ gestation) were instrumented to measure umbilical, carotid, pulmonary and femoral arterial flows and pressures. Systemic and cerebral oxygenation was recorded using pulse oximetry and near-infrared spectroscopy, respectively. Fetal asphyxia was induced until mean blood pressure reached ~20 mm Hg, where lambs underwent ICC and initiation of ventilation (n=7), or ventilation for 15 min prior to umbilical cord clamping (PBCC; n=8). Cardiovascular parameters were measured and white and grey matter microvascular integrity assessed using qRT-PCR and immunohistochemistry.ResultsPBCC restored oxygenation and cardiac output at the same rate and in a similar fashion to lambs resuscitated following ICC. However, ICC lambs had a rapid and marked overshoot in mean systemic arterial blood pressure from 1 to 10 min after ventilation onset, which was largely absent in PBCC lambs. ICC lambs had increased cerebrovascular injury, as indicated by reduced expression of blood–brain barrier proteins and increased cerebrovascular protein leakage in the subcortical white matter (by 86%) and grey matter (by 47%).ConclusionPBCC restored cardiac output and oxygenation in an identical time frame as ICC, but greatly mitigated the postasphyxia rebound hypertension measured in ICC lambs. This likely protected the asphyxiated brain from cerebrovascular injury. PBCC may be a more suitable option for the resuscitation of the asphyxiated newborn compared with the current standard of ICC.
Neonatal mannequin comparison of the Upright self-inflating bag and snap-fit mask versus standard resuscitators and masks: leak, applied load and tidal volumes
Authors: Rafferty Anthony Richard , Johnson Lucy , Davis Peter G , Dawson Jennifer Anne , Thio Marta , Owen Louise S 
Published: November 2017

ObjectiveNeonatal mask ventilation is a difficult skill to acquire and maintain. Mask leak is common and can lead to ineffective ventilation. The aim of this study was to determine whether newly available neonatal self-inflating bags and masks could reduce mask leak without additional load being applied to the face.DesignForty operators delivered 1 min episodes of mask ventilation to a mannequin using the Laerdal Upright Resuscitator, a standard Laerdal infant resuscitator (Laerdal Medical) and a T-Piece Resuscitator (Neopuff), using both the Laerdal snap-fit face mask and the standard Laerdal size 0/1 face mask (equivalent sizes). Participants were asked to use pressure sufficient to achieve ‘appropriate’ chest rise. Leak, applied load, airway pressure and tidal volume were measured continuously. Participants were unaware that load was being recorded.ResultsThere was no difference in mask leak between resuscitation devices. Leak was significantly lower when the snap-fit mask was used with all resuscitation devices, compared with the standard mask (14% vs 37% leak, P<0.01). The snap-fit mask was preferred by 83% of participants. The device-mask combinations had no significant effect on applied load.ConclusionsThe Laerdal Upright Resuscitator resulted in similar leak to the other resuscitation devices studied, and did not exert additional load to the face and head. The snap-fit mask significantly reduced overall leak with all resuscitation devices and was the mask preferred by participants.
Genetic deletion of the circadian clock transcription factor BMAL1 and chronic alcohol consumption differentially alter hepatic glycogen in mice
Authors: Udoh Uduak S. , Valcin Jennifer A. , Swain Telisha M. , Filiano Ashley N. , Gamble Karen L. , Young Martin E. , Bailey Shannon M. 
Published: November 2017

Multiple metabolic pathways exhibit time-of-day-dependent rhythms that are controlled by the molecular circadian clock. We have shown that chronic alcohol is capable of altering the molecular clock and diurnal oscillations in several elements of hepatic glycogen metabolism ( 19 , 44 ). Herein, we sought to determine whether genetic disruption of the hepatocyte clock differentially impacts hepatic glycogen content in chronic alcohol-fed mice. Male hepatocyte-specific BMAL1 knockout (HBK) and littermate controls were fed control or alcohol-containing diets for 5 wk to alter hepatic glycogen content. Glycogen displayed a significant diurnal rhythm in livers of control genotype mice fed the control diet. While rhythmic, alcohol significantly altered the diurnal oscillation of glycogen in livers of control genotype mice. The glycogen rhythm was mildly altered in livers of control-fed HBK mice. Importantly, glycogen content was arrhythmic in livers of alcohol-fed HBK mice. Consistent with these changes in hepatic glycogen content, we observed that some glycogen and glucose metabolism genes were differentially altered by chronic alcohol consumption in livers of HBK and littermate control mice. Diurnal rhythms in glycogen synthase (mRNA and protein) were significantly altered by alcohol feeding and clock disruption. Alcohol consumption significantly altered Gck, Glut2, and Ppp1r3g rhythms in livers of control genotype mice, with diurnal rhythms of Pklr, Glut2, Ppp1r3c, and Ppp1r3g further disrupted (dampened or arrhythmic) in livers of HBK mice. Taken together, these findings show that chronic alcohol consumption and hepatocyte clock disruption differentially influence the diurnal rhythm of glycogen and various key glycogen metabolism-related genes in the liver.NEW & NOTEWORTHY We report that circadian clock disruption exacerbates alcohol-mediated alterations in hepatic glycogen. We observed differential responsiveness in diurnal rhythms of glycogen and glycogen metabolism genes and proteins in livers of hepatocyte-specific BMAL1 knockout and littermate control mice fed alcohol. Our findings provide new insights into potential mechanisms by which alcohol alters glycogen, an important energy source for liver and other organs.