bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine Learning Prediction of Pancreatitis Risk With Antithyroid Drugs: A Nationwide Retrospective Observational Study
Authors: Kizilgul Muhammed , Yigit Talip , Ata Naim , Celik Ahmet , Ucan Bekir , Sahin Mustafa , Ulgu M Mahir , Birinci Suayip 
Published: September 2024

Abstract

Background
In recent years, there has been increasing data showing that the risk of acute pancreatitis (AP) is increased in patients using methimazole (MMI). The aim of this population-based study was to investigate the association between drugs used in the treatment of hyperthyroidism (MMI, propylthiouracil, propranolol) and the diagnosis of AP.

Material and Methods
The database consisted of more than 85 million citizen records between January 1, 2017, and December 31, 2022. Within the scope of the study, the data were examined of patients diagnosed with hyperthyroidism and the individuals who developed acute pancreatitis after being diagnosed with hyperthyroidism, which was accomplished using the International Classification of Diseases, Tenth Revision codes. Analyses were carried out using R Studio 4.2.0 software. The test-train technique as a machine learning method was used in Cox regression models.

Results
A total of 1 329 934 individuals diagnosed with hyperthyroidism were examined. Of these, 10 815 (0.81%) patients developed AP after being diagnosed with hyperthyroidism. The use of propylthiouracil was determined to increase the probability of experiencing AP diagnosis 0.10-fold (10%) (P .0009), and the use of propranolol decreased the probability of experiencing AP 0.5-fold (5%) (P .032), with a 52.2% predictive success rate. There is no any statistically significant association between MMI use and the probability of experiencing AP according to the model we built.

Conclusion
Although the risk of experiencing AP was not increased by MMI use, propylthiouracil use increased and propranolol use decreased the risk of experiencing AP in patients with hyperthyroidism. Although drug-induced AP is relatively rare, a firm understanding of the drugs associated with the condition should alert the clinician to appropriately diagnose and treat patients.
Comprehensive Study on Central Precocious Puberty: Molecular and Clinical Analyses in 90 Patients
Authors: Narusawa Hiromune , Ogawa Tomoe , Yagasaki Hideaki , Nagasaki Keisuke , Urakawa Tatsuki , Saito Tomohiro , Soneda Shun , Kinjo Saori , Sano Shinichiro , Mamada Mitsukazu , Terashita Shintaro , Dateki Sumito , Narumi Satoshi , Naiki Yasuhiro , Horikawa Reiko , Ogata Tsutomu , Fukami Maki , Kagami Masayo 
Published: September 2024

Abstract

Context
Defects in MKRN3, DLK1, KISS1, and KISS1R and some disorders, such as Temple syndrome (TS14), cause central precocious puberty (CPP). Recently, pathogenic variants (PVs) in MECP2 have been reported to be associated with CPP.

Objective
We aimed to clarify the contribution of (epi)genetic abnormalities to CPP and clinical and hormonal features in each etiology.

Methods
We conducted targeted sequencing for MKRN3, DLK1, MECP2, KISS1, and KISS1R and methylation analysis for screening of imprinting disorders such as TS14 associated with CPP in 90 patients with CPP (no history of brain injuries and negative brain magnetic resonance imaging) and collected their clinical and laboratory data. We measured serum DLK1 levels in 3 patients with TS14 and serum MKRN3 levels in 2 patients with MKRN3 genetic defects, together with some etiology-unknown patients with CPP and controls.

Results
We detected 8 patients with TS14 (6, epimutation; 1, mosaic maternal uniparental disomy chromosome 14; 1, microdeletion) and 3 patients with MKRN3 genetic defects (1, PV; 1, 13-bp deletion in the 5′-untranslated region [5′-UTR]; 1, microdeletion) with family histories of paternal early puberty. There were no patients with PVs identified in MECP2, KISS1, or KISS1R. We confirmed low serum MKRN3 level in the patient with a deletion in 5′-UTR. The median height at initial evaluation of TS14 patients was lower than that of all patients. Six patients with TS14 were born small for gestational age (SGA).

Conclusion
(Epi)genetic causes were identified in 12.2% of patients with CPP at our center. For patients with CPP born SGA or together with family histories of paternal early puberty, (epi)genetic testing for TS14 and MKRN3 genetic defects should be considered.
Weight-Specific Grip Strength as a Novel Indicator Associated With Cardiometabolic Risk in Children: The EMSNGS Study
Authors: Li Zhuoguang , Wu Chushan , Song Yongfang , Li Yanyan , Zhao Xiu , Shang Yue , Zheng Rongfei , Su Huiping , Su Qiru , Li Yan , Fu Rongyin , Lu Wenlong , Xiong Jingfan , Su Zhe 
Published: September 2024

Abstract

Context
Handgrip strength (HGS) is an important indicator of sarcopenia and adverse health outcomes. However, evaluating HGS in children presents challenges, and its association with metabolism remains incompletely understood.

Objective
To establish grip strength reference values for Chinese children and adolescents, as well as to evaluate the relationship between HGS and cardiometabolic risk.

Methods
Data were collected from 4 072 participants aged 6-18 as part of the Evaluation and Monitoring on School-based Nutrition and Growth in Shenzhen (EMSNGS) study. HGS was measured, and relative HGS (RHGS) was normalized by body mass index. Age- or weight-specific HGS and RHGS were derived using the generalized additive model of location, scale, and shape model, and participants’ values were categorized into quartiles, defining low strength as the lowest quartile. The cardiometabolic risk index (CMRI) z-score was calculated, with high risk defined as a z-score of ≥1.

Results
Both boys and girls exhibited similar increases in age- and weight-specific grip strength. Low grip strength, classified by weight-specific HGS and RHGS, was linked to higher CMRI z-scores than classifications based on age-specific references in both sexes. A dose-dependent relationship was observed between weight-specific grip strength and cardiometabolic risk, particularly in boys. Compared with the middle category (P25th–P75th), the odds ratios for high cardiometabolic risks associated with low grip strength increased in both sexes.

Conclusion
This study established grip strength reference values for Chinese youth, introduced the concept of weight-specific HGS and RHGS, and demonstrated a dose-dependent relationship between weight-specific grip strength and cardiometabolic risk. These findings highlighted the association between low muscle strength and increased cardiometabolic risk.
Population Growth, Immigration, and Labor Market Dynamics
Authors: Elsby Michael W. L. , Smith Jennifer C. , Wadsworth Jonathan 
Published: September 2024

Abstract
This article provides a first synthesis of population flows and labor market dynamics across immigrant and native-born populations. We devise a novel dynamic accounting methodology that integrates population flows from two sources—changes in birth cohort size and immigrant flows—with labor market dynamics. We illustrate the method using data for the United Kingdom, where population flows have been large and cyclical, driven first by the maturation of baby boom cohorts in the 1980s and later by immigration in the 2000s. New measures of labor market flows by migrant status uncover the flow origins of disparities in the levels and cyclicality of immigrant and native labor market outcomes and their more recent convergence. An application of our accounting framework reveals that population flows have played a nontrivial role in the volatility of labor markets among the UK-born and, especially, immigrants.
The Seed Keepers
Authors: Sen Pooja 
Published: September 2024

Abstract
Seeds always presuppose cooperative arrangements of tending and care. Seeds are useful to think with because they connect the past, present, and future, carrying old biologies and inherited histories as well as genetic transformations and new possibilities. This essay traces a history of botanical classification in herbariums, photographs, and seed libraries in Palestine that stretches back to the nineteenth century. Through individuation and taxonomic ordering, colonial botanical archives isolated plants and people from history and vanished Palestinians from their land. The colonial archive's formal organization and orientation to preserving dead or disappearing objects also opens space to investigate the proliferation of archive-based artworks by contemporary Palestinian artists such as Larissa Sansour and Jumana Manna. Sansour and Manna's artworks demonstrate that the value of seeds lies in their cultivation, transformations, and local adaptations. The seed is an alternative archive that maintains the vitality of being in the world. The seed is well-suited to planting Palestinian futures that are living, growing, and changing.
Robust organ size in Arabidopsis is primarily governed by cell growth rather than cell division patterns
Authors: Burda Isabella , Brauns Fridtjof , Clark Frances K. , Li Chun-Biu , Roeder Adrienne H. K. 
Published: September 2024

ABSTRACT
Organ sizes and shapes are highly reproducible, or robust, within a species and individuals. Arabidopsis thaliana sepals, which are the leaf-like organs that enclose flower buds, have consistent size and shape, indicating robust development. Cell growth is locally heterogeneous due to intrinsic and extrinsic noise. To achieve robust organ shape, fluctuations in cell growth must average to an even growth rate, which requires that fluctuations are uncorrelated or anti-correlated in time and space. Here, we live image and quantify the development of sepals with an increased or decreased number of cell divisions (lgo mutant and LGO overexpression, respectively), a mutant with altered cell growth variability (ftsh4), and double mutants combining these. Changes in the number of cell divisions do not change the overall growth pattern. By contrast, in ftsh4 mutants, cell growth accumulates in patches of over- and undergrowth owing to correlations that impair averaging, resulting in increased organ shape variability. Thus, we demonstrate in vivo that the number of cell divisions does not affect averaging of cell growth, preserving robust organ morphogenesis, whereas correlated growth fluctuations impair averaging.
Environmental and molecular control of tissue-specific ionocyte differentiation in zebrafish
Authors: Peloggia Julia , Lush Mark E. , Tsai Ya-Yin , Wood Christopher , Piotrowski Tatjana 
Published: September 2024

ABSTRACT
Organisms cope with environmental fluctuations and maintain fitness in part via reversible phenotypic changes (acclimation). Aquatic animals are subject to dramatic seasonal fluctuations in water salinity, which affect osmolarity of their cells and consequently cellular function. Mechanosensory lateral line hair cells detect water motion for swimming behavior and are especially susceptible to salinity changes due to their direct contact with the environment. To maintain hair cell function when salinity decreases, neuromast (Nm)-associated ionocytes differentiate and invade lateral line neuromasts. The signals that trigger the adaptive differentiation of Nm ionocytes are unknown. We demonstrate that new Nm ionocytes are rapidly specified and selectively triggered to proliferate by low Ca2+ and Na+/Cl− levels. We further show that Nm ionocyte recruitment and induction is affected by hair cell activity. Once specified, Nm ionocyte differentiation and survival are associated with sequential activation of different Notch pathway components, a process different from other tissue-specific ionocytes. In summary, we show how environmental changes activate a signaling cascade that leads to physiological adaptation. This may prove essential for survival not only in seasonal changing environments but also in changing climates.
Ribosome biogenesis is essential for hemogenic endothelial cells to generate hematopoietic stem cells
Authors: Liu Di , Wang Haizhen , Chen Haifeng , Tian Xitong , Jiao Yuqing , Wang Chi , Li Yuhui , Li Zongcheng , Hou Siyuan , Ni Yanli , Liu Bing , Lan Yu , Zhou Jie 
Published: September 2024

ABSTRACT
Undergoing endothelial-to-hematopoietic transition, a small fraction of embryonic aortic endothelial cells specializes into hemogenic endothelial cells (HECs) and eventually gives rise to hematopoietic stem cells (HSCs). Previously, we found that the activity of ribosome biogenesis (RiBi) is highly enriched in the HSC-primed HECs compared with adjacent arterial endothelial cells; however, whether RiBi is required in HECs for the generation of HSCs remains to be determined. Here, we have found that robust RiBi is markedly augmented during the endothelial-to-hematopoietic transition in mouse. Pharmacological inhibition of RiBi completely impeded the generation of HSCs in explant cultures. Moreover, disrupting RiBi selectively interrupted the HSC generation potential of HECs rather than T1 pre-HSCs, which was in line with its influence on cell cycle activity. Further investigation revealed that, upon HEC specification, the master transcription factor Runx1 dramatically bound to the loci of genes involved in RiBi, thereby facilitating this biological process. Taken together, our study provides functional evidence showing the indispensable role of RiBi in generating HSCs from HECs, providing previously unreported insights that may contribute to the improvement of HSC regeneration strategies.
Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties
Authors: Woudenberg Sjoerd , Alvarez Melissa Dipp , Rienstra Juriaan , Levitsky Victor , Mironova Victoria , Scarpella Enrico , Kuhn Andre , Weijers Dolf 
Published: September 2024

ABSTRACT
The auxin signaling molecule regulates a range of plant growth and developmental processes. The core transcriptional machinery responsible for auxin-mediated responses is conserved across all land plants. Genetic, physiological and molecular exploration in bryophyte and angiosperm model species have shown both qualitative and quantitative differences in auxin responses. Given the highly divergent ontogeny of the dominant gametophyte (bryophytes) and sporophyte (angiosperms) generations, however, it is unclear whether such differences derive from distinct phylogeny or ontogeny. Here, we address this question by comparing a range of physiological, developmental and molecular responses to auxin in both generations of the model fern Ceratopteris richardii. We find that auxin response in Ceratopteris gametophytes closely resembles that of a thalloid bryophyte, whereas the sporophyte mimics auxin response in flowering plants. This resemblance manifests both at the phenotypic and transcriptional levels. Furthermore, we show that disrupting auxin transport can lead to ectopic sporophyte induction on the gametophyte, suggesting a role for auxin in the alternation of generations. Our study thus identifies developmental phase, rather than phylogeny, as a major determinant of auxin response properties in land plants.
Expanding the field of view – a simple approach for interactive visualisation of electron microscopy data
Authors: Wohlmann Jens 
Published: September 2024

ABSTRACT
The unparalleled resolving power of electron microscopy is both a blessing and a curse. At 30,000× magnification, 1 µm corresponds to 3 cm in the image and the field of view is only a few micrometres or less, resulting in an inevitable reduction in the spatial data available in an image. Consequently, the gain in resolution is at the cost of loss of the contextual ‘reference space’, which is crucial for understanding the embedded structures of interest. This problem is particularly pronounced in immunoelectron microscopy, where the detection of a gold particle is crucial for the localisation of specific molecules. The common solution of presenting high-magnification and overview images side by side often insufficiently represents the cellular environment. To address these limitations, we propose here an interactive visualization strategy inspired by digital maps and GPS modules which enables seamless transitions between different magnifications by dynamically linking virtual low magnification overview images with primary high-resolution data. By enabling dynamic browsing, it offers the potential for a deeper understanding of cellular landscapes leading to more comprehensive analysis of the primary ultrastructural data.
A single-particle analysis method for detecting membrane remodelling and curvature sensing
Authors: Colussi Adeline , Almeida-Souza Leonardo , McMahon Harvey T. 
Published: September 2024

ABSTRACT
In biology, shape and function are related. Therefore, it is important to understand how membrane shape is generated, stabilised and sensed by proteins and how this relates to organelle function. Here, we present an assay that can detect curvature preference and membrane remodelling with free-floating liposomes using protein concentrations in physiologically relevant ranges. The assay reproduced known curvature preferences of BAR domains and allowed the discovery of high-curvature preference for the PH domain of AKT and the FYVE domain of HRS (also known as HGS). In addition, our method reproduced the membrane vesiculation activity of the ENTH domain of epsin-1 (EPN1) and showed similar activity for the ANTH domains of PiCALM and Hip1R. Finally, we found that the curvature sensitivity of the N-BAR domain of endophilin inversely correlates to membrane charge and that deletion of its N-terminal amphipathic helix increased its curvature specificity. Thus, our method is a generally applicable qualitative method for assessing membrane curvature sensing and remodelling by proteins.
Intrinsic religiosity and purchase intention: The role of attitude and moral efficacy
Authors: Suseno Andi , Bidayati Utik , Purwoko Purwoko , Waeno Mahamadaree 
Published: September 2024

The diversity of products on the market today raises various kinds of consumer considerations when making purchases. Consumers will consider the basics they have before making a purchase. Factors that influence consumers before making a purchase come from internal and external consumers. In this study, consumer behavior analysis is seen based on internal factors such as intrinsic religiosity, attitude, and moral efficacy. This study analyzes the effect of intrinsic religiosity on purchase intention by mediating attitude and moral efficacy. This study examines the behavior of consumers of cosmetic products in Yogyakarta, Indonesia and obtained a sample of 92 respondents. The research data were analyzed using the Smart PLS 4.0 software to test validity, reliability, and bootstrapping. The results of the study proved intrinsic religiosity does not negatively affect purchase intention, intrinsic religiosity has no positive effect on attitude, intrinsic religiosity has a positive effect on moral efficacy, attitude negatively affects purchase intention, moral efficacy has no negative effect on purchase intention, attitude does not mediate the influence of intrinsic religiosity on purchase intention, and moral efficacy did not mediate the effect of intrinsic religiosity on purchase intention.
RVN's perceptions of confidence levels of veterinary professionals when assessing pain in rabbits
Authors: Barton Abigail , Harniman Suzannah 
Published: September 2024

Background: Despite the popularity of pet rabbits in the UK, recent research suggests limitations in veterinary professionals’ knowledge and experience regarding behaviour and pain assessment in these patients. There is also a lack of species-specific pain assessment scales for rabbits. Methods: An online questionnaire collected key data, followed by semi-structured, one-to-one interviews over Microsoft Teams with five registered veterinary nurses. Thematic analysis was used to interpret the qualitative data. Results: Five themes were identified. Participants with more experience with rabbits felt more confident assessing their pain. Some participants found it difficult to distinguish between stress and pain when assessing body language in rabbit patients using pain assessment scales. Conclusions: To improve the accuracy of pain assessment in rabbits, it is recommended that registered veterinary nurses and veterinary surgeons receive more training and experience with the species. The potential impact could include more appropriate use of analgesia and improved welfare.
Levels and functionality of Pacific Islanders’ hybrid humoral immune response to BNT162b2 vaccination and delta/omicron infection: A cohort study in New Caledonia
Authors: Inizan Catherine , Courtot Adrien , Sturmach Chloé , Griffon Anne-Fleur , Biron Antoine , Bruel Timothée , Enouf Vincent , Demaneuf Thibaut , Munier Sandie , Schwartz Olivier , Gourinat Ann-Claire , Médevielle Georges , Jouan Marc , van der Werf Sylvie , Madec Yoann , Albert-Dunais Valérie , Dupont-Rouzeyrol Myrielle 
Published: September 2024

Background
Pacific Islanders are underrepresented in vaccine efficacy trials. Few studies describe their immune response to COVID-19 vaccination. Yet, this characterization is crucial to re-enforce vaccination strategies adapted to Pacific Islanders singularities.

Methods and findings
We evaluated the humoral immune response of 585 adults, self-declaring as Melanesians, Europeans, Polynesians, or belonging to other communities, to the Pfizer BNT162b2 vaccine. Anti-spike and anti-nucleoprotein IgG levels, and their capacity to neutralize SARS-CoV-2 variants and to mediate antibody-dependent cellular cytotoxicity (ADCC) were assessed across communities at 1 and 3 months post-second dose or 1 and 6 months post-third dose. All sera tested contained anti-spike antibodies and 61.3% contained anti-nucleoprotein antibodies, evidencing mostly a hybrid immunity resulting from vaccination and SARS-CoV-2 infection. At 1-month post-immunization, the 4 ethnic communities exhibited no significant differences in their anti-spike IgG levels (p value = 0.17, in an univariate linear regression model), in their capacity to mediate omicron neutralization (p value = 0.59 and 0.60, in an univariate logistic regression model at 1-month after the second and third dose, respectively) and in their capacity to mediate ADCC (p value = 0.069 in a multivariate linear regression model), regardless of the infection status. Anti-spike IgG levels and functionalities of the hybrid humoral immune response remained equivalent across the 4 ethnic communities during follow-up and at 6 months post-third dose.

Conclusions
Our study evidenced Pacific Islander’s robust humoral immune response to Pfizer BNT162b2 vaccine, which is pivotal to re-enforce vaccination deployment in a population at risk for severe COVID-19.

Trial registration
This trial has been register in ClinicalTrials.gov (ID: NCT05135585).