bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Host miRNA Hsa-miR-139-3p in HPV-16–Induced Carcinomas
Authors: Sannigrahi M.K. , Sharma Rajni , Singh Varinder , Panda Naresh K. , Rattan Vidya , Khullar Madhu 
Published: February 2017

Abstract
Purpose: Human papillomavirus 16 (HPV-16) is an important risk factor in head and neck cancer (HNC). Studies suggest that miRNAs play an important role in cancer; however, their role in HPV-mediated oncogenesis remains largely unknown. We investigated the role of miRNAs with HPV-16 as putative target in HPV-16–mediated cancers.
Experimental Design: Using in silico tools, we identified miRNAs with putative binding sequences on HPV-16 miRNAs. Hsa-miR-139-3p was identified as best candidate miRNA by luciferase reporter assay and was found to be significantly downregulated in HPV-16–positive tissues and cell lines. Overexpression/inhibition studies were performed to determine the role of miRNA in regulating oncogenic pathways.
Results: Hsa-miR-139-3p was found to target high-risk HPV-16 oncogenic proteins and revive major tumor suppressor proteins (p53, p21, and p16). This resulted in inhibition of cell proliferation and cell migration, cell-cycle arrest at G2–M phase and increased cell death of HPV-16–positive cells. Analysis of The Cancer Genome Atlas (TCGA) data showed decreased expression of Hsa-miR-139-3p in HPV-16–positive HNC and cervical cancer cases, and its higher expression correlated with better survival outcome in both cases. Increased DNA methylation of Hsa-miR-139-3p harboring gene PDE2A at its promoter/CpG islands was observed in HPV-16–positive tissues and cell lines, which further correlated with Hsa-miR-139-3p expression, suggesting its role in regulating Hsa-miR-139-3p expression. Furthermore, we observed an increased sensitization of Hsa-miR-139-3p overexpressed HPV-16–positive cells to chemotherapeutic drugs (cisplatin and 5-fluorouracil).
Conclusions: HPV-16–mediated downregulation of Hsa-miR-139-3p may promote oncogenesis in HNC and cervical cancer. Clin Cancer Res; 23(14); 3884–95. ©2017 AACR.
Analysis of Five-Level DSTATCOM for Harmonic Reduction in Power System Due To Non-Linear Loads
Authors: Raveendra N. , Madhusudhan V. , Jayalaxmi A. 
Published: February 2017

<p class="AEEEAbstract"><strong></strong>This paper deals with the elimination of harmonics at PCC in the presence of non-linear type of loads. Introduction of five-level DSTATCOM at PCC injects compensating currents for the minimization of harmonic content in the source currents. Non-linear loads when connected to the system draw non-linear components of current leaving undesired harmonics at the source side. DSTATCOM when connected to the system at the coupling point induces compensating currents to nullify harmonics. This paper introduces the simulation modelling of five-level DSTATCOM for harmonic elimination. Matlab/Simulink modelling was done for the system without DSTATCOM and its harmonic contents were shown. The system without DSTATCOM is compared with the system with the presence of DSTATCOM showing their respective THD in the source current. Five level DSTATCOM was validated by considering fixed load and variable load. Simulation work was carried out for three types of systems and results were obtained using Matlab/Simulink.</p>
Learning Vector Quantization Image for Identification Adenium
Authors: Wulanningrum Resty , Robby Bagus Fadzerie 
Published: February 2017

Information and technology are two things that can not be separated and it has become a necessity for human life. Technology development at this time was not only used for intelligence purposes only, but has penetrated the world of holtikurtura. Adenium is one of the plants are much favored by ornamental plants lovers. Many of cultivation adenium who crosses that appear new varieties that have the color and shape are similar to each other. From this case, then made an application that can identify the type of adenium based on the image of that flower. Learning Vector quantization is one of the algorithm that used for clustering. Based on test scenarios were performed, image identification applications Adenium petals produce an accuracy of 86.66% with a number of training dataset of 135 images and datasets with a test as many as 45 images max epoch 10 and learning rate between 0.01 to 0.05.
Analysis and Design of High Performance Phase Frequency Detector, Charge Pump and Loop Filter Circuits for Phase Locked Loop in Wireless Applications
Authors: Metange P.N. , Khanchandani K. B. 
Published: February 2017

<p>This paper presents the analysis and design of high performance phase frequency detector, charge pump and loop filter circuits for phase locked loop in wireless applications. The proposed phase frequency detector (PFD) consumes only 8 µW and utilises small area. Also, at 1.8V voltage supply the maximum operation frequency of the conventional PFD is 500 MHz whereas proposed PFD is 5 GHz. Hence, highly suitable for low power, high speed and low jitter applications. The differential charge pump uses switches using NMOS and the inverter delays for up and down signals do not generate any offset due to its fully symmetric operation. This configuration doubles the range of output voltage compliance compared to single ended charge pump. Differential stage is less sensitive to the leakage current since leakage current behaves as common mode offset with the dual output stages. All the circuits are implemented using cadence 0.18 μm CMOS Process.</p>
In Memoriam Edward Louis Keenan Jr.
Authors: Martin Russell E. 
Published: February 2017

On Friday, September 25, 2015, in Memorial Church at Harvard University, a memorial service was held to celebrate the life and career of Professor Edward L. Keenan Jr., Andrew W. Mellon Professor of History, Emeritus, who passed away on March 6, 2015. The memorial service gathered together hundreds – family members, former colleagues and students, and countless friends. The eight speakers at this memorial described Edward (Ned) Keenan’s influence on them, but also his inestimable impact on the field of Russian history; and his widow provided a window into his thinking about the many roles he played over the course of a diverse and significant career that spanned four decades. The memorial service marked a sad moment in the field of Russian studies, and the words offered at it are important for understanding how the field of Russian history in the United States grew and was transformed in the last quarter of the twentieth century.
Seasonal Transitions and the Westerly Jet in the Holocene East Asian Summer Monsoon
Authors: Kong Wenwen , Swenson Leif M. , Chiang John C. H. 
Published: February 2017

The Holocene East Asian summer monsoon (EASM) was previously characterized as a trend toward weaker monsoon intensity paced by orbital insolation. It is demonstrated here that this evolution is more accurately characterized as changes in the transition timing and duration of the EASM seasonal stages (spring, pre-mei-yu, mei-yu, midsummer), and tied to the north–south displacement of the westerlies relative to Tibet. To this end, time-slice simulations across the Holocene are employed using an atmospheric general circulation model. Self-organizing maps are used to objectively identify the transition timing and duration of the EASM seasonal stages.Compared to the late Holocene, an earlier onset of mei-yu and an earlier transition from mei-yu to midsummer in the early to mid-Holocene are found, resulting in a shortened mei-yu and prolonged midsummer stage. These changes are accompanied by an earlier northward positioning of the westerlies relative to Tibet. Invoking changes to seasonal transitions also provides a more satisfactory explanation for two key observations of Holocene East Asian climate: the “asynchronous Holocene optimum” and changes to dust emissions.A mechanism is proposed to explain the altered EASM seasonality in the simulated early to mid-Holocene. The insolation increase over the boreal summer reduces the pole–equator temperature gradient, leading to northward-shifted and weakened westerlies. The meridional position of the westerlies relative to the Tibetan Plateau determines the onset of mei-yu and possibly the onset of the midsummer stage. The northward shift in the westerlies triggers earlier seasonal rainfall transitions and, in particular, a shorter mei-yu and longer midsummer stage.
A Little Anthropomorphism Goes a Long Way
Authors: de Visser Ewart J. , Monfort Samuel S. , Goodyear Kimberly , Lu Li , O’Hara Martin , Lee Mary R. , Parasuraman Raja , Krueger Frank 
Published: February 2017

Objective: We investigated the effects of exogenous oxytocin on trust, compliance, and team decision making with agents varying in anthropomorphism (computer, avatar, human) and reliability (100%, 50%). Background: Authors of recent work have explored psychological similarities in how people trust humanlike automation compared with how they trust other humans. Exogenous administration of oxytocin, a neuropeptide associated with trust among humans, offers a unique opportunity to probe the anthropomorphism continuum of automation to infer when agents are trusted like another human or merely a machine. Method: Eighty-four healthy male participants collaborated with automated agents varying in anthropomorphism that provided recommendations in a pattern recognition task. Results: Under placebo, participants exhibited less trust and compliance with automated aids as the anthropomorphism of those aids increased. Under oxytocin, participants interacted with aids on the extremes of the anthropomorphism continuum similarly to placebos but increased their trust, compliance, and performance with the avatar, an agent on the midpoint of the anthropomorphism continuum. Conclusion: This study provides the first evidence that administration of exogenous oxytocin affected trust, compliance, and team decision making with automated agents. These effects provide support for the premise that oxytocin increases affinity for social stimuli in automated aids. Application: Designing automation to mimic basic human characteristics is sufficient to elicit behavioral trust outcomes that are driven by neurological processes typically observed in human–human interactions. Designers of automated systems should consider the task, the individual, and the level of anthropomorphism to achieve the desired outcome.
Predicting Racial and Ethnic Disparities in Advance Care Planning Using the Integrated Behavioral Model
Authors: McAfee Colette A. , Jordan Timothy R. , Sheu Jiunn-Jye (J. J.) , Dake Joseph A. , Kopp Miller Barbara A. 
Published: February 2017

Purpose To explain and predict racial or ethnic disparities in advance care planning (ACP) behaviors among American adults by using the Integrated Behavioral Model (IBM) and the Precaution Adoption Process Model. Methods A randomized, observational, nonexperimental, cross-sectional study design was used to survey American adults between 40 and 80 years of age ( n = 386). Results The majority of respondents (75%) had not completed ACP. Significant differences were found by race or ethnicity: 33% of Whites had completed ACP versus Hispanics (18%) and Blacks (8%). Whites had statistically significantly higher levels of most IBM constructs compared with Blacks and Hispanics. The IBM predicted 28% of the variance in behavioral intention. After controlling for sociodemographic variables, direct attitudes, indirect attitudes, and indirect perceived norms were significant predictors of behavioral intention. Conclusion The IBM and the Precaution Adoption Process Model are useful frameworks for interventions designed to increase ACP among racial or ethnic minorities in the United States.
Effects on pain of a stepwise multidisciplinary intervention (STA OP!) that targets pain and behavior in advanced dementia: A cluster randomized controlled trial
Authors: Pieper Marjoleine JC , van der Steen Jenny T , Francke Anneke L , Scherder Erik JA , Twisk Jos WR , Achterberg Wilco P 
Published: February 2017

Background: Pain in nursing home residents with advanced dementia remains a major challenge; it is difficult to detect and may be expressed as challenging behavior. STA OP! aims to identify physical and other needs as causes of behavioral changes and uses a stepwise approach for psychosocial and pharmacological management which was effective in improving challenging behavior. Aim: To assess whether implementation of the stepwise multidisciplinary intervention also reduces pain and improves pain management. Design: In a cluster randomized controlled trial (Netherlands National Trial Register NTR1967), healthcare professionals of intervention units received the stepwise training, while training of the control group focused on knowledge and skills without the stepwise component. Observed and estimated pain was assessed at baseline and at 3 and 6 months post-intervention. Logistic generalized estimating equations were used to test treatment and time effects. Setting/participants: A total of 21 clusters (single nursing home units) in 12 Dutch nursing homes included 288 residents with advanced dementia (Global Deterioration Scale score 5, 6, or 7): 148 in the intervention and 140 in the control condition. Results: The multilevel modeling showed an overall effect of the intervention on observed pain but not on estimated pain; Pain Assessment Checklist for Seniors with Limited Ability to Communicate–Dutch version, mean difference: −1.21 points (95% confidence interval: −2.35 to −0.06); Minimum Dataset of the Resident Assessment Instrument pain scale, mean difference: −0.01 points (95% confidence interval: −0.36 to 0.35). Opioid use increased (odds ratio = 3.08; 95% confidence interval: 1.08–8.74); paracetamol use did not (odds ratio = 1.38; 95% confidence interval: 0.71–2.68). Conclusion: STA OP! was found to decrease “observed” pain but not estimated pain. Observing pain-related behavior might help improve pain management in dementia.
Effects of acute and repeated treatment with the biased mu opioid receptor agonist TRV130 (oliceridine) on measures of antinociception, gastrointestinal function, and abuse liability in rodents
Authors: Altarifi Ahmad A , David Bethany , Muchhala Karan H , Blough Bruce E , Akbarali Hamid , Negus S Stevens 
Published: February 2017

Rationale: TRV130 (oliceridine; N-[(3-methoxythiophen-2-yl)methyl]-2-[(9 R)-9-pyridin-2-yl-6-oxaspiro[4.5]decan-9-yl]ethanamine) is a novel mu opioid receptor (MOR) agonist that preferentially activates G-protein versus β-arrestin signaling pathways coupled to MORs. Prevailing evidence suggests that TRV130 and other G-protein-biased MOR agonists may produce therapeutic analgesic effects with reduced adverse effects compared to existing MOR agonists. Objectives: This study compared the effects of acute and repeated TRV130 administration on measures of antinociception, gastrointestinal function, and abuse liability in rodents. We hypothesized that TRV130 would produce robust and sustained antinociception and abuse-related effects during repeated treatment, but that tolerance would develop to gastrointestinal inhibition. Methods: Antinociception was assessed using a warm-water tail-withdrawal procedure in mice. Gastrointestinal function was assessed in mice using an in vivo measure of fecal output and in vitro assays of colonic propulsion and of colon and ileum circular muscle contraction. Abuse liability was assessed in rats using an intracranial self-stimulation (ICSS) procedure. (+)-TRV130 was administered with acute and repeated dosing regimens, and (–)-TRV130 was also examined in the ICSS procedure to assess stereoselectivity. Results: Acute (+)-TRV130 treatment produced robust antinociception, complete inhibition of gastrointestinal function, and weak abuse-related effects. Repeated (+)-TRV130 treatment failed to produce tolerance to antinociception or gastrointestinal inhibition, and abuse-related effects were enhanced by repeated treatment. Effects of acute and repeated (+)-TRV130 in these procedures resemble effects of morphine, with the exception that TRV130 antinociception was more resistant to tolerance. (–)-TRV130 was inactive. Conclusions: These results suggest that TRV130 retains undesirable constipating and abuse-related effects during repeated treatment despite its bias for G-protein signaling.
Does Allograft Augmentation of Small-Diameter Hamstring Autograft ACL Grafts Reduce the Incidence of Graft Retear?
Authors: Pennock Andrew T. , Ho Brian , Parvanta Kristina , Edmonds Eric W. , Chambers Henry G. , Roocroft Joanna H. , Bastrom Tracey P. 
Published: February 2017

Background: Small-diameter hamstring tendons are frequently encountered during anterior cruciate ligament (ACL) reconstructions in patients with short stature or those who are skeletally immature. The role of augmenting these small-diameter autografts with allograft is unclear. Purpose: To assess clinical outcomes and failure rates in adolescent patients with small hamstring tendon autografts (<7 mm) that were either augmented with soft tissue allograft or accepted “as is” and not augmented. Study Design: Cohort study; Level of evidence, 3. Methods: A retrospective chart review of all primary ACL reconstructions performed with hamstring autografts identified 385 patients, of whom 50 (13%) had a quadrupled (semitendinosus-gracilis) graft size less than 7 mm. Patients were grouped based on the surgeon’s preference either to augment these grafts with allograft (augmented group; n = 26) or to accept the smaller autograft (nonaugmented group; n = 24). Preoperative demographic, injury, and intraoperative data were documented. All patients were contacted to obtain information about outcome scores, subsequent procedures, and complications. Forty patients (20 in each group) were available for 2-year follow-up. Results: The mean age of the entire cohort was 15.7 years (range, 12-18 years), and 38% were male. No between-group differences were found with respect to any of the preoperative or intraoperative variables except extremity side. The mean graft size for the augmented group was 8.9 mm and for the nonaugmented group was 6.4 mm. At a mean follow-up of 3 years, 6 (30%) of the patients in the augmented group had a graft failure, whereas only 1 (5%) in the nonaugmented group had a failure ( P = .04). Five of the 6 augmented failures occurred within 1 year of surgery, whereas the single failure in the nonaugmented group occurred 2.7years postoperatively. No differences were noted in the reported outcomes between patients in the augmented and nonaugmented groups who did not experience graft failure (Lysholm score, 88 vs 92; Tegner score, 6.4 vs 6.3; single-assessment numeric evaluation score, 86 vs 86; satisfaction, 8.4 vs 8.9, respectively). Conclusion: Adolescents undergoing an ACL reconstruction frequently have small hamstring tendon autograft size. The augmentation of these small grafts with allograft does not reduce graft failure rates and may in fact lead to higher retear rates, with earlier graft failure.
Incidence and Radiographic Predictors of Valgus Tibiotalar Tilt After Hindfoot Fusion
Authors: Miniaci-Coxhead Sara Lyn , Weisenthal Benjamin , Ketz John P. , Flemister A. Samuel 
Published: February 2017

Background: The development of valgus tibiotalar tilt following hindfoot arthrodesis is rarely discussed in the literature. The purpose of this study was to determine the incidence of valgus tibiotalar tilt and to evaluate if there were any radiographic predictors for the development of valgus tibiotalar tilt. Methods: Patients who underwent hindfoot fusion between January 1, 2004 and December 31, 2013 were identified. Charts were reviewed for demographic information and operative details. Preoperative and postoperative radiographs were reviewed for the development of tibiotalar tilt, and standardized measurements and angles were calculated. A total of 187 patients were included. There were 106 (56.7%) females and 81 (43.3%) males. The average age was 52 years (range, 11-82 years). The most common indication for surgery was adult-acquired flatfoot deformity (n = 92, 49.2%), followed by arthritis (n = 83, 44.4%). The most common procedure was triple arthrodesis (n = 101, 54%). Twenty-seven patients demonstrated tibiotalar tilt preoperatively. Results: A total of 51 patients (27.3%) developed valgus tibiotalar tilt postoperatively at an average of 3.6 months after surgery. We found that an increase in the preoperative Meary (lateral talar–first metatarsal) angle (hazard ratio, 1.039; 95% confidence interval, 1.002-1.077; P < .05) was associated with the development of tibiotalar tilt. An increase in the postoperative Meary angle (hazard ratio, 1.052; 95% confidence interval, 0.999-1.108; P = .0528) approached significance for the development of tibiotalar tilt. Conclusion: The development of valgus tibiotalar tilt following hindfoot fusion was a notable phenomenon, occurring in 27% of our patient population. The preoperative Meary angle was the only radiographic measurement that was significantly associated with the development of valgus tibiotalar tilt, although the postoperative Meary angle approached significance. These findings should encourage surgeons to be aware of patients with large deformities and of their propensity to develop a valgus deformity about the ankle. Level of Evidence: Level III, retrospective comparative series.
Adolescent patellar instability
Authors: Clark D. , Metcalfe A. , Wogan C. , Mandalia V. , Eldridge J. 
Published: February 2017

Patellar instability most frequently presents during adolescence. Congenital and infantile dislocation of the patella is a distinct entity from adolescent instability and measurable abnormalities may be present at birth. In the normal patellofemoral joint an increase in quadriceps angle and patellar height are matched by an increase in trochlear depth as the joint matures. Adolescent instability may herald a lifelong condition leading to chronic disability and arthritis.Restoring normal anatomy by trochleoplasty, tibial tubercle transfer or medial patellofemoral ligament (MPFL) reconstruction in the young adult prevents further instability. Although these techniques are proven in the young adult, they may cause growth arrest and deformity where the physis is open. A vigorous non-operative strategy may permit delay of surgery until growth is complete. Where non-operative treatment has failed a modified MPFL reconstruction may be performed to maintain stability until physeal closure permits anatomical reconstruction. If significant growth remains an extraosseous reconstruction of the MPFL may impart the lowest risk to the physis. If minor growth remains image intensifier guided placement of femoral intraosseous fixation may impart a small, but acceptable, risk to the physis.This paper presents and discusses the literature relating to adolescent instability and provides a framework for management of these patients.Cite this article: Bone Joint J 2017;99-B:159–70.