bluuebunny/crossref_metadata_2025_split huggingface dataset:
The small RNA biogenesis in rice is regulated by MAP kinase‐mediated OsCDKD phosphorylation
Authors: Singh Dhanraj , Verma Neetu , Rengasamy Balakrishnan , Banerjee Gopal , Sinha Alok Krishna 
Published: September 2024

Summary

CDKs are the master regulator of cell division and their activity is controlled by the regulatory subunit cyclins and phosphorylation by the CAKs. However, the role of MAP kinases in regulating plant cell cycle or CDKs have not been explored.

Here, we report that the MAP kinases OsMPK3, OsMPK4, and OsMPK6 physically interact and phosphorylate OsCDKD and its regulatory subunit OsCYCH in rice. MAP kinases phosphorylate CDKD at Ser‐168 and Thr‐235 residues in OsCDKD. The MAP kinase‐mediated phosphorylation of OsCDKD is required for its activation to control the small RNA biogenesis. The phosphodead version of OsCDKD fails to activate the C‐terminal domain of RNA Polymerase II, thereby negatively impacting small RNA transcription.

Further, the overexpression lines of wild‐type (WT) OsCDKD and phosphomimic OsCDKD show increased root growth, plant height, tiller number, panicle number, and seed number in comparison to WT, phosphodead OsCDKD‐OE, and kinase‐dead OsCDKD‐OE plants.

In a nutshell, our study establishes a novel regulation of OsCDKD by MAPK‐mediated phosphorylation in rice. The phosphorylation of OsCDKD by MAPKs imparts a positive effect on rice growth and development by regulating miRNAs transcription.
Predicting soil hydraulic conductivity using random forest, SVM, and LSSVM models
Authors: Farasati Masumeh , Seyedian Morteza , Fathaabadi Abolhasan 
Published: September 2024

AbstractUnderstanding the hydraulic properties of soil is essential to solve many management problems in agriculture and the environment. Water quality affects soil hydraulic conductivity. Soil hydraulic properties play an important role in nature's water cycle and are used as basic information in designing irrigation and drainage systems, hydrological issues, and soil quality assessment. In the current study, soil sampling is performed from different areas and its hydraulic conductivity was measured using the drop load method and then predicted using support vector machine (SVM) and least‐squares support vector machine (LSSVM) models. The model inputs were: soil texture (percentage of sand, silt, and clay particles), salinity (electrical conductivity), pH, sodium adsorption ratio, soil porosity, and bulk density and the output was soil hydraulic conductivity. Correlation coefficient, root mean square error (RMSE), mean bias error (MBE), and Nash–Sutcliffe efficiency (NSE) were used to evaluate the models and compare them. Based on evaluation criteria the best performance was obtained for random forest (RF) (R = 0.89, RMSE = 0.53, mean absolute error (MAE) = 0.54, and NSE = 0.72). Following RF, the SVM with (R = 0.69, RMSE = 1.32, MAE = 0.69, and NSE = 0.48) performed better than LSSVM model.
Exploring Plant Diversity Through Enzyme‐Mediated Analysis Using Electro‐Carbon Sensors
Authors: Rachappanavar Vinaykumar 
Published: September 2024

ABSTRACTThis review explores the application of enzyme‐mediated analysis using electro‐carbon sensors to investigate plant diversity. Precision plant genotype fingerprinting (PPGF) represents a paradigm shift in agricultural science, merging the fields of phyto‐enzyme identification and quantification with cutting‐edge electro‐sensor technology. PPGF acts as a powerful tool for dissecting the genetic makeup of plant species by meticulously examining their unique phyto‐enzyme signatures. Electro‐sensor technology emerges as a pioneering force, utilizing electro‐analytical methods to precisely measure and differentiate these markers. Enzyme‐based sensors are capable of detecting plant metabolites even at low concentrations, enabling highly precise and accurate measurements. Furthermore, they are generally more eco‐friendly because they use fewer hazardous chemicals and produce less waste. These sensors can function under gentle conditions and can be miniaturized, making them highly suitable for field applications. This synergistic approach between PPGF and electro‐sensor technology ushers in a transformative era, offering unprecedented insights into plant genomics and paving the way for novel avenues for PPGF. Overall, enzyme‐based electrochemical sensors contribute to improving the efficiency and effectiveness of plant fingerprinting, thereby facilitating more comprehensive and precise plant phenotyping and research endeavours.
Tyrosylprotein Sulfotransferase Positively Regulates Symbiotic Nodulation and Root Growth
Authors: Zhang Danping , Di Qi , Gui Jinshan , Li Qiong , Mysore Kirankumar S. , Wen Jiangqi , Luo Li , Yu Liangliang 
Published: September 2024

ABSTRACTPosttranslational tyrosine sulfation of peptides and proteins is catalysed by tyrosylprotein sulfotransferases (TPSTs). In Arabidopsis, tyrosine sulfation is essential for the activities of peptide hormones, such as phytosulfokine (PSK) and root meristem growth factor (RGF). Here, we identified a TPST‐encoding gene, MtTPST, from model legume Medicago truncatula. MtTPST expression was detected in all organs, with the highest level in root nodules. A promoter:GUS assay revealed that MtTPST was highly expressed in the root apical meristem, nodule primordium and nodule apical meristem. The loss‐of‐function mutant mttpst exhibited a stunted phenotype with short roots and reduced nodule number and size. Application of both of the sulfated peptides PSK and RGF3 partially restored the defective root length of mttpst. The reduction in symbiotic nodulation in mttpst was partially recovered by treatment with sulfated PSK peptide. MtTPST‐PSK module functions downstream of the Nod factor signalling to promote nodule initiation via regulating accumulation and/or signalling of cytokinin and auxin. Additionally, the small‐nodule phenotype of mttpst, which resulted from decreased apical meristematic activity, was partially complemented by sulfated RGF3 treatment. Together, these results demonstrate that MtTPST, through its substrates PSK, RGF3 and other sulfated peptide(s), positively regulates nodule development and root growth.
Wittgenstein's critical Philosophy of Mathematical Practice
Authors: Scheppers Frank 
Published: September 2024

AbstractOn the one hand, I show that the later Wittgenstein's practice‐based approach to meaning, including the idea that the meaningfulness of mathematics ultimately is rooted in the everyday ‘applications’ it emerged from, as well as his insistence on the variability in and contingency of mathematical and mathematics‐like practices, foreshadows more recent work in Philosophy of Mathematical Practice (PMP), although Wittgenstein's approach was more radically practice‐based than what is prevalent in present‐day PMP. On the other hand, I also show that Wittgenstein's work on mathematics is driven by a pervasive critical attitude towards some key characteristics (monism, foundationalism and exceptionalism) of mainstream philosophical discourse about mathematics (then and now), which is mostly absent from present‐day PMP, and I argue that this aspect of Wittgenstein's work on mathematics could inspire present‐day practitioners of PMP to rethink the identity of their discipline.
Gratitude and believing in someone
Authors: Lewis Max 
Published: September 2024

AbstractI aim to vindicate the claim that we can owe someone gratitude for believing in us and to show how this seemingly prosaic fact has important upshots for the normativity of gratitude. I start by sketching a novel account of what it is to believe in someone according to which it consists in holding an affective attitude of confident optimism toward their general ability in some domain(s). I then argue that people can deserve gratitude for holding this attitude. I close by showing how the possibility of being gratitudeworthy for believing in someone casts down on three commonplaces about when gratitude is owed.
Emotions and the phenomenal grasping of epistemic blameworthiness
Authors: Magalotti Tricia 
Published: September 2024

AbstractIn this paper, I consider the potential implications of the observation that epistemic judgment seems to be less emotional than moral judgment. I argue that regardless of whether emotions are necessary for blame, blaming emotions do play an important epistemic role in the moral domain. They allow us to grasp propositions about moral blameworthiness and thereby to appreciate their significance in a special way. Further, I argue that if we generally lack blaming emotions in the epistemic domain, then we are unable to grasp propositions about epistemic blameworthiness. As a result, regardless of one's theory of epistemic blame, there emerges a tension between the claims that we are epistemically blameworthy for our epistemic failings and the claim that we do not feel epistemic blaming emotions.
Blaming the victim
Authors: Sliwa Paulina 
Published: September 2024

AbstractFeminists critique acts and practices as victim‐blaming. Victim‐blaming is a moral phenomenon: to call a communicative act victim blaming is to criticise it. It is also a political phenomenon. As feminists point out, it plays a important role in perpetuating oppression. But what makes a communicative act an act of victim‐blaming? I propose that victim‐blaming communicative acts attribute responsibility to the victim for the wrong in contexts in which such attributions are morally improper. Attributions of responsibility can be morally imporoper in virtue of what they make salient in a conversation. Making salient the victim's conduct and backgrounding the conduct of the perpetrator can run afoul of the duties we have to the victim: including the duty to listen to the victim, to support her, and to hold the perpetrator responsible.
Life cycle and molecular phylogeny of Vaucheria piloboloides (Vaucheriales, Xanthophyceae) from Sado Island, Japan
Authors: Hoshino Masakazu , Tadokoro Satoru , Akita Shingo , Uwai Shinya 
Published: September 2024

SUMMARYThe siphonous, yellow–green alga Vaucheria piloboloides (Vaucheriales, Xanthophyceae) is the type species of the section Piloboloideae and is widely reported worldwide. Despite its taxonomic importance and broad range distribution, its detailed life cycle and molecular phylogenetic position are unknown. In the present study, we established culture strains of V. piloboloides collected from Sado Island, Niigata Prefecture, Japan, and examined its morphology, life cycle and phylogenetic position based on rbcL sequences. The morphology of the gametangia and aplanosporangia (asexual sporangia) of our samples, along with their monoecious nature, resembled that of V. piloboloides from the Atlantic coast, including the type locality. The rbcL phylogenetic analysis demonstrated that our sample formed a clade with other Piloboloideae species, supporting the current sectional scheme of Vaucheria. Under culture conditions, our samples exhibited a monophasic life cycle. Development of gametangia was observed in any culture conditions, whereas development of aplanosporangia could be induced by low salinity (salinity = 24).
Governing the European Union's recovery and resilience facility: National ownership and performance‐based financing in theory and practice
Authors: Zeitlin Jonathan , Bokhorst David , Eihmanis Edgars 
Published: September 2024

AbstractThe Recovery and Resilience Facility (RRF) adopted in response to the COVID‐19 pandemic marks an important departure in European Union (EU) governance, as it introduces an innovative “demand‐driven, performance‐based” model aimed at overcoming the limitations of past policies seeking to promote national reforms. In this study, we set out the theoretical assumptions underlying the RRF governance model, and assess its practical effectiveness and legitimacy by analyzing the drafting, implementation, and monitoring of National Recovery and Resilience Plans in eight member states. The study concludes by assessing the strengths and weaknesses of the RRF's governance model, relating them to theoretical expectations derived from previous international experience with similar approaches elsewhere, and considers the implications for future EU policy. Our core argument is that while the RRF's governance design has reinforced national ownership and commitment to reform and investment objectives, its performance‐based financing system leads to a mechanical focus on formal verification of predetermined milestones and targets, with negative consequences for both effectiveness and legitimacy. Addressing these problems would require a redesign of the RRF's complete contracting approach, giving member states greater flexibility on the means for achieving agreed commitments, as well as for revising them, not only in response to unanticipated changes in objective circumstances, but also to lessons learned during the implementation process.
On recognizing the real: Beauty and affliction in Simone Weil
Authors: Thomas Christopher 
Published: September 2024

AbstractIf the guiding question of ethics is “how should I live?,” then the guiding question of aesthetics might be “what is beauty?” For Simone Weil, these two questions have intertwined answers that turn on a like conceptual apparatus. Focussing on Weil's foremost ethical problem, the plight of the afflicted (malheur), this article offers an account of the philosophical basis to Weil's claim that, when truly recognized, beauty and affliction motivate the same form of experience. I argue that, for Weil, both the aesthetic and moral disposition are grounded in an experience of the real, an experience that both requires and compels the subject to the same attentive state. Finally, I address the charges that might be leveled at Weil for suggesting that our experience of beauty is the same as our experience of affliction, specifically arguing that rather than this experience leading to a form of passive aesthetic arrest, as the experience of beauty has typically been theorized to lead to, Weil understands the experience of beauty and affliction as fundamentally motivational toward the Good.
Attitudes toward abortion legality and abortion regulation: Insights from a nationally representative study
Authors: Jozkowski Kristen N. , Crawford Brandon L. , Hawbaker Amelia , Parker Erik , Golzarri Arroyo Lilian , Turner Ronna C. 
Published: September 2024

AbstractObjectiveNational public opinion polls and surveys use different questions from one another to assess people's abortion attitudes. We included commonly asked abortion attitude items on a single survey to examine people's attitudes toward abortion legality and abortion restriction to create profiles of people which we then compared across state groups. Concurrently assessing attitudes toward both abortion legality and restrictions is important given the changing abortion legislative climate in the United States.MethodWe administered an online survey to U.S. adults (n = 919) via Ipsos probability panel and used latent class analysis to identify classes of participants. Then, we used multinominal logistic regression to make state‐level comparisons.ResultsWe identified three classes: (1) 35.0 percent—abortion should be illegal/more restricted, (2) 35.1 percent—abortion should be legal/laws should reflect the status quo, and (3) 29.9 percent—abortion should be legal/more available. Trigger‐law states comprise the largest proportion of people who think abortion should be illegal/more restricted, whereas states without trigger laws comprise similar proportions of people from all three classes.ConclusionConcurrently measuring whether people believe abortion should be legal and the extent it should be restricted can provide a more comprehensive understanding of people's attitudes and demonstrates important state‐level nuances in attitudes.
Region‐specific diversification dynamics and biogeographic history of one of the most diverse families of insects
Authors: Ghanavi Hamid Reza , Chazot Nicolas , Sanmartín Isabel , Murillo‐Ramos Leidys , Duchêne Sebastián , Sihvonen Pasi , Brehm Gunnar , Wahlberg Niklas 
Published: September 2024

AbstractA long‐standing question in evolutionary biology is how historical biogeographic processes have shaped the current diversity of organisms, especially for highly diverse groups. We study the diversification dynamics and biogeographic processes of one of the most diverse families of Lepidoptera, Geometridae, with over 24,000 described species and a worldwide distribution. Despite the cosmopolitan distribution of the family, most species of Geometridae have limited distribution ranges. We present the largest historical biogeography and diversification study on the current diversity patterns and distribution ranges of Geometridae. We use a multi‐locus dataset of 1200 taxa to estimate the historical biogeography of Geometridae, implementing a Bayesian approach of the Dispersal‐Extinction‐Cladogenesis (DEC) model that incorporates palaeographic‐based dispersal graphs with uncertainty in geological ages in RevBayes. We also implement a Bayesian time‐variable, episodic birth–death model and a model that allows branch‐specific speciation rates to estimate the diversification dynamics in the family. Our results suggest that the most recent common ancestor of Geometridae was distributed in the New World, with the Neotropics being the most likely ancestral area. An increase in diversification rates occurred circa 30–40 million years ago (Mya), coinciding with a time of a major global climate cooling in the Eocene. Clade‐specific shifts in speciation rates also occurred around 10–15 Mya, coincident with another period of major climate change in the Oligocene. Our results point to different biogeographical and evolutionary histories per area to show the differences of the diversification rates in different biogeographical regions through time, showing the relative importance of each region in the diversification history of Geometridae.
The development and evanescence of red blood cell antibodies after transfusion: A multi‐institutional prospective study in Japan
Authors: Yamada Chiaki , Ono Takaaki , Ino Kaede , Nemoto Naoki , Shinba Takahito , Furumaki Hiroaki , Shibata Hiroki , Ishizuka Keiko , Yamada Naotomo , Matsuura Hideaki , Izuhara Yumiko , Fujihara Harumi , Minamiguchi Hitoshi 
Published: September 2024

AbstractBackgroundDespite several reports on red blood cell (RBC) alloimmunization, the actual prevalence and factors contributing to RBC alloimmunization in transfused patients remain poorly investigated. We examined the association between clinical factors and the development and evanescence of RBC antibodies after transfusion.Study Design and MethodsEach participating institution performed antibody screens before and after RBC transfusion. A survey including patient characteristics, results of antibody screen and identification, antibody screen methods, total amount of RBC transfused, and adverse reactions, was conducted.ResultsBetween October 2018 and March 2023, 1194 patients were registered at five institutions. Overall, 958 patients underwent at least one follow‐up RBC antibody screen after transfusion, revealing new antibody development in 44 (4.6%). Anti‐E was identified in 25 patients, anti‐Jka in 5, and anti‐c in 4. The number of RBC units transfused was significantly associated with antibody development after transfusion (p < .001). Among 55 patients in whom antibodies were identified after transfusion, including historical antibodies, antibodies evanesced in 18 (33%); anti‐E in 7, anti‐Jka in 4, and anti‐Lea in 2. Evanescent antibodies were identified more frequently by saline and/or enzyme methods than persistent antibodies (p = .012).DiscussionThe number of RBC units transfused can impact antibody development, and antibodies identified only by saline and/or enzyme methods, deemed clinically insignificant, are likely to have a high evanescence rate. Antibody screen should be carefully performed, especially in those receiving a large number of RBC units. Confirming previous antibody screen results should be performed to prevent omitting evanesced antibodies regardless of clinical relevance.
A Retrospective Study Evaluating the Outcomes of Conventionally Fractionated Radiation Therapy as a Treatment for Infiltrative Lipomas in Twenty‐One Dogs
Authors: Hauser Adam , Thorsen Lily , Boss Mary‐Keara , Martin Tiffany W. 
Published: September 2024

ABSTRACTInfiltrative lipomas represent a subcategorisation of rarer, potentially more aggressive, lipoma‐related neoplasms. Twenty‐one dogs treated with conventionally fractionated radiotherapy (CFRT) for infiltrative lipomas were included in this retrospective study. One patient had no prior surgical excision, 11 patients had one prior surgery and 9 patients had two or more surgeries prior to CFRT. Five patients (24%) had microscopic disease and 16 patients (76%) had macroscopic disease prior to treatment. A complete response or no regrowth was seen in 10 patients (48%), stable disease in 6 patients (29%) and progressive disease or regrowth in 5 patients (24%). Response to treatment of macroscopic tumours was significantly different between dogs that had one prior surgery versus two or more (p = 0.01). Dogs with a single surgery were most likely to result in stable disease compared with dogs with two or more surgeries resulting in a complete response. The dog without surgery developed progressive disease at 211 days, dogs with one surgery had a median progression or recurrence at 1369 days and dogs with two or more surgeries developed progression or recurrence at 826 days (p = 0.04). Twelve dogs were alive at the time of analysis. Overall median survival time (MST) was 1694 days. The prior number of surgeries did not significantly affect MST. While survival time is comparable to previous reports, the number of patients with progressive disease or recurrence of previous microscopic disease requires more investigation into the most appropriate protocol, dose and treated field size.
Impact of regression modeling on the assessment and harmonization of a point‐of‐care analyzer and commercial laboratory analyzer for feline plasma biochemistry testing
Authors: Baral Randolph M. , Flatland Bente , Jaensch Susan M. , Hayward Douglas A. , Freeman Kathleen P. 
Published: September 2024

AbstractBackgroundRegression describes the relationship of results from two analyzers, and the generated equation can be used to harmonize results. Point‐of‐care (POC) analyzers cannot be calibrated by the end user, so regression offers an opportunity for calculated harmonization. Harmonization (uniformity) of laboratory results facilitates the use of common reference intervals and medical decision thresholds.ObjectiveOur aims were to characterize the relationship of results for multiple biochemistry analytes on a POC and a commercial laboratory analyzer (CL) with three regression techniques and to use regression equations to harmonize the POC results with those of the CL. Harmonized results were assessed by recognized quality goals. We used harmonized results to assess the regression techniques.MethodsAfter analyzer imprecision assessments, paired clinical samples were assessed with one dataset to calculate regression parameters that were applied to a second dataset. Three regression techniques were performed, and each was used to harmonize the POC results with those from the CL. POC results were assessed for bias and the number of results reaching quality goals before and after harmonization.ResultsAll regression techniques could be used to harmonize most analytes so that 95% of results were within ASVCP TEa guidelines. Harmonization could be further improved with alternate regression techniques or exclusions.ConclusionsRegression offers a means to harmonize POC and CL analyzers. Further work is needed to assess how few samples can reliably be used and to assess likely species differences. No regression technique reliably describes the relationship between methods when correlation is poor.