bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observations and Simulations of a Double‐Core Hot Flow Anomaly
Authors: Lu Xi , Otto Antonius , Zhang Hui , Liu Terry , Chen Xingran 
Published: September 2024

AbstractHot Flow anomalies (HFAs), one of the most well‐analyzed transient phenomena in the Earth's foreshock, are known as kinetic structures driven by tangential discontinuities (TDs). Recently, a 2‐dimensional (2D) magnetohydrodynamics (MHD) model reproduced HFAs with either a high‐ or low‐density core. Further investigation of an HFA with two cores observed by the Magnetospheric Multiscale (MMS) mission is reported. The observation via the Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon's Interaction with the Sun (ARTEMIS) mission suggests this MHD HFA is associated with a foreshock density hole‐like structure. The trailing flux tube in simulation may propagate with a TD in the foreshock. Our work suggests that HFAs with two low‐density cores can also be achieved in MHD process. Results show the total ram pressure can be an excellent diagnostic for the presence of transient structures, such as HFAs, at the bow shock.
Madden‐Julian Oscillation Contributes to the Skewed Intraseasonal PNA in El Niño and La Niña Winters
Authors: Zhou Fang , Jian Shenghua , Liu Minghong , Wang Run 
Published: September 2024

AbstractThe impact of the Madden‐Julian oscillation (MJO) on the intraseasonal PNA (ISPNA) was investigated and was found to be modulated by the El Niño‐Southern Oscillation (ENSO), which reasonably explains the skewness of the ISPNA during El Niño and La Niña winters. It was shown that the intensity and periodicity of the ISPNA was much stronger and slightly longer in La Niña winters than in the El Niño winters. The phase‐locked association between the ISPNA and MJO indicate that this skewness was controlled by the MJO. The northward Rossby wave activities derived from the tropics associated with the MJO to the subtropical Pacific sector of the ISPNA clarified that the stronger intensity of the MJO convection in the western Pacific during the La Niña winters, as well as the slower eastward propagation of the MJO, led to the asymmetric intensity and period of the ISPNA in the two ENSO phases.
Tracking vaccine effectiveness in an evolving pandemic, countering misleading hot takes and epidemiologic fallacies
Authors: Morris Jeffrey S 
Published: September 2024

Abstract
With the emergence of Omicron during the pandemic and the establishment of antibody waning over time, vaccine effectiveness, especially against infection, declined sharply from the original levels seen after the initial rollout. However, studies have demonstrated that they still provided substantial protection vs severe/fatal disease even with Omicron and after waning. Social media has been rife with reports claiming vaccines provided no benefit and some even claiming they made things worse, often driven by simple presentations of raw observational data using erroneous arguments involving epidemiologic fallacies including the base rate fallacy, Simpson’s paradox, and the ecological fallacy and ignoring the extensive bias especially from confounding that is an inherent feature of these data. Similar fallacious arguments have been made by some in promoting vaccination policies, as well. Generally, vaccine effectiveness cannot be accurately estimated from raw population summaries but instead require rigorous, careful studies using epidemiologic designs and statistical analysis tools attempting to adjust for key confounders and sources of bias. This article summarizes what aggregated evidence across studies reveals about effectiveness of the mRNA vaccines as the pandemic has evolved, chronologically summarized with emerging variants and highlighting some of the fallacies and flawed arguments feeding social media-based claims that have obscured society’s collective understanding.
Low foetal heart rate, a potentially ominous finding: case report
Authors: Afana Andreea Sorina , Filip Cristina , Cimpoca Brindusa , Dumitrascu-Biris Ioana , Jurcut Ruxandra 
Published: September 2024

Abstract

Background
Congenital long QT syndrome (LQTS) type 1 is characterized by abnormally prolonged ventricular repolarization caused by inherited defects in cardiac potassium channels. Patients are predisposed to ventricular arrhythmias and even sudden cardiac death. In some cases, foetal sinus bradycardia is the only sign, making prenatal diagnosis challenging. Physicians should be aware of this subtle presentation of LQTS. Early diagnosis and proactive treatment are crucial for preventing unexpected cardiac events.

Case summary
A healthy and asymptomatic 25-year-old pregnant woman was referred to our institute for cardiac evaluation after persistent foetal sinus bradycardia was detected during repeated ultrasounds, despite the absence of any foetal morphological or functional cardiac anomalies. After a thorough assessment, the mother was diagnosed with LQTS type 1, as confirmed by molecular genetic testing. Appropriate management, including maternal medication and increased surveillance, was initiated. The infant was delivered safely, and his electrocardiogram revealed a significantly prolonged QTc interval. Genetic testing confirmed the maternally inherited variant in KCNQ1 gene, and beta-blocker therapy was started. No arrhythmic events were noted.

Discussion
Detection and careful stratification of foetal heart rate (FHR) is crucial in every pregnancy. Foetal bradycardia can be caused by both maternal and foetal factors. Persistent low FHR should raise a high suspicion for LQTS. The condition may also present with atrioventricular blocks, torsades de pointes, or sudden intrauterine foetal demise. Accurate and early diagnosis of LQTS is essential for implementing appropriate management strategies, which include vigilant monitoring, effective medical treatment, careful planning of delivery, and post-natal care.
Aortic valve replacement vs. conservative treatment in asymptomatic severe aortic stenosis: long-term follow-up of the AVATAR trial
Authors: Banovic Marko , Putnik Svetozar , Da Costa Bruno R , Penicka Martin , Deja Marek A , Kotrc Martin , Kockova Radka , Glaveckaite Sigita , Gasparovic Hrvoje , Pavlovic Nikola , Velicki Lazar , Salizzoni Stefano , Wojakowski Wojtek , Van Camp Guy , Gradinac Sinisa , Laufer Michael , Tomovic Sara , Busic Ivan , Bojanic Milica , Ristic Arsen , Klasnja Andrea , Matkovic Milos , Boskovic Nikola , Zivic Katarina , Jovanovic Miodrag , Nikolic Serge D , Iung Bernard , Bartunek Jozef 
Published: September 2024

Abstract

Background and Aims
The question of when and how to treat truly asymptomatic patients with severe aortic stenosis (AS) and normal left ventricular (LV) systolic function is still subject to debate and ongoing research. Here, the results of extended follow-up of the AVATAR trial are reported (NCT02436655, ClinicalTrials.gov).

Methods
The AVATAR trial randomly assigned patients with severe, asymptomatic AS and LV ejection fraction ≥ 50% to undergo either early surgical aortic valve replacement (AVR) or conservative treatment with watchful waiting strategy. All patients had negative exercise stress testing. The primary hypothesis was that early AVR will reduce a primary composite endpoint comprising all-cause death, acute myocardial infarction, stroke, or unplanned hospitalization for heart failure (HF), as compared with conservative treatment strategy.

Results
A total of 157 low-risk patients (mean age 67 years, 57% men, mean Society of Thoracic Surgeons score 1.7%) were randomly allocated to either the early AVR group (n = 78) or the conservative treatment group (n = 79). In an intention-to-treat analysis, after a median follow-up of 63 months, the primary composite endpoint outcome event occurred in 18/78 patients (23.1%) in the early surgery group and in 37/79 patients (46.8%) in the conservative treatment group [hazard ratio (HR) early surgery vs. conservative treatment 0.42; 95% confidence interval (CI) 0.24–0.73, P = .002]. The Kaplan–Meier estimates for individual endpoints of all-cause death and HF hospitalization were significantly lower in the early surgery compared with the conservative group (HR 0.44; 95% CI 0.23–0.85, P = .012, for all-cause death and HR 0.21; 95% CI 0.06–0.73, P = .007, for HF hospitalizations).

Conclusions
The extended follow-up of the AVATAR trial demonstrates better clinical outcomes with early surgical AVR in truly asymptomatic patients with severe AS and normal LV ejection fraction compared with patients treated with conservative management on watchful waiting.
Darwin’s overlooked radiation: genomic evidence points to the early stages of a radiation in the Galápagos prickly pear cactus (Opuntia: Cactaceae)
Authors: Zapata Felipe , Cerca José , McCarney Dana , Henriquez Claudia L , Tiamiyu Bashir B , McCormack John E , Reckling Kelsey R , Chaves Jaime A , Rivas-Torres Gonzalo 
Published: September 2024

Abstract
In the Galápagos Islands, much attention has been devoted to the radiation of iconic species like Darwin’s finches, yet the Galápagos Islands offer an overlooked but equally remarkable opportunity for investigating plant radiations. Using a combination of genomic and phenotypic data, we present evidence of the early stages of a radiation in prickly pear cactus (Opuntia), a lineage widespread across the archipelago. We show that despite extensive ecophenotypic variation, there is limited genomic differentiation, consistent with the hypothesis that Opuntia is in the early stages of the diversification process. Phylogenomic and population genomic analyses show that notwithstanding low genetic differentiation across islands, there is marked geographical structure that is broadly consistent with the palaeogeography of the Galápagos. Because low genetic differentiation stands in stark contrast to the exceptional eco-phenotypic diversity displayed by cacti, it is plausible that the genetic architecture of phenotypic divergence mismatches our genomic sequencing, that phenotypic plasticity precedes genetic divergence and is the source of adaptive evolution, or that introgression influences local adaptation. Models of population relationships including admixture indicate that gene flow is common, probably facilitated by dispersal via animals known to feed on Opuntia flowers, fruits, and seeds. Because the prickly pear cacti of the Galápagos are a radiation in the making, they provide an exciting opportunity to investigate the interplay between ecological and genomic mechanisms promoting diversification.
Unde venis? Bacterial resistance from environmental reservoirs to lettuce: tracking microbiome and resistome over a growth period
Authors: Gekenidis Maria-Theresia , Vollenweider Vera , Joyce Aoife , Murphy Sinéad , Walser Jean-Claude , Ju Feng , Bürgmann Helmut , Hummerjohann Jörg , Walsh Fiona , Drissner David 
Published: September 2024

Abstract
Fresh produce is suggested to contribute highly to shaping the gut resistome. We investigated the impact of pig manure and irrigation water quality on microbiome and resistome of field-grown lettuce over an entire growth period. Lettuce was grown under four regimes, combining soil amendment with manure (with/without) with sprinkler irrigation using river water with an upstream wastewater input, disinfected by UV (with/without). Lettuce leaves, soil, and water samples were collected weekly and analysed by bacterial cultivation, 16S rRNA gene amplicon sequencing, and shotgun metagenomics from total community DNA. Cultivation yielded only few clinically relevant antibiotic-resistant bacteria (ARB), but numbers of ARB on lettuce increased over time, while no treatment-dependent changes were observed. Microbiome analysis confirmed a temporal trend. Antibiotic resistance genes (ARGs) unique to lettuce and water included multidrug and β-lactam ARGs, whereas lettuce and soil uniquely shared mainly glycopeptide and tetracycline ARGs. Surface water carried clinically relevant ARB (e.g. ESBL-producing Escherichia coli or Serratia fonticola) without affecting the overall lettuce resistome significantly. Resistance markers including biocide and metal resistance were increased in lettuce grown with manure, especially young lettuce (increased soil contact). Overall, while all investigated environments had their share as sources of the lettuce resistome, manure was the main source especially on young plants. We therefore suggest minimizing soil–vegetable contact to minimize resistance markers on fresh produce.
Testing treecbh in Central European forests: an R package for crown base height detection using high-resolution aerial laser-scanned data
Authors: Diószegi Gergő , Molnár Vanda Éva , Nagy Loránd Attila , Enyedi Péter , Török Péter , Szabó Szilárd 
Published: September 2024

Abstract
Accurate information regarding tree canopy characteristics is crucial for forest management, but it is often difficult to assess. This study presents an innovative framework designed for crown base height (CBH) detection using high-resolution laser-scanned data, with a specific focus on individual trees within forests. The framework comprises three key steps: (i) segmenting the input tree point cloud to identify the tree trunk and its branches using the treesio software; (ii) applying vertical cross-sectional K-means clustering to cluster the identified tree and to define the elevation threshold for removing low-lying understory vegetation; (iii) employing a novel 2D kernel method for detecting CBH after eliminating low-lying understory vegetation. The 2D kernel method, developed for broadleaf forests using leaf-off airborne laser scanning (ALS) data, underpins the treecbh tool. This tool features a visual CBH adjustment component that shows a 2D profile plot of the tree point cloud, and suggests a CBH value for user approval or adjustment. To evaluate accuracy, in situ measured CBH data from five forest plots in Germany and Hungary with varied species compositions were used. ALS data were collected during leaf-off conditions for the two Hungarian plots and during leaf-on conditions for the three German plots. Leaf-off terrestrial laser-scanned data from individual trees were also used in the accuracy assessment. A sensitivity analysis using random point decimation was conducted on the terrestrial laser-scanned data to assess treecbh’s sensitivity to point density. The initial results exhibited matching rates of 45% and 60% for leaf-off ALS plots, which significantly improved to 71% and 77%, respectively, when using the visual CBH adjustment feature of the tool. The leaf-on ALS results demonstrated matching rates between 24% and 33%, whereas the CBHs of individual terrestrial laser-scanned trees could be detected with 93% accuracy in visual mode. It was observed that treecbh operates effectively when the input ALS data have a minimum point density of 20 pts/${\text{m}}^2$, with its optimal performance achieved at 110 pts/${\text{m}}^2$. These findings indicated treecbh’s sensitivity to ALS data quality, scanning season (leaf-on and leaf-off), and point density. This sensitivity can be effectively mitigated in the case of leaf-off ALS data by utilizing the visual CBH adjustment feature of the tool.
TRIM25 activates Wnt/β-catenin signalling by destabilising MAT2A mRNA to drive thoracic aortic aneurysm development
Authors: Li Chaojie , Wang Kan , Fang Jian , Qin Lin , Ling Qiong , Yu Yu 
Published: September 2024

Abstract
This study explored the roles of methionine adenosyltransferase 2A (MAT2A) and tripartite motif containing 25 (TRIM25) in the progression of thoracic aortic aneurysm (TAA). The TAA model was established based on the β-aminopropionitrile method. The effects of MAT2A on thoracic aortic lesions and molecular levels were analyzed by several pathological staining assays (hematoxylin–eosin, Verhoeff-Van Gieson, TUNEL) and molecular biology experiments (qRT-PCR, Western blot). Angiotensin II (Ang-II) was used to induce injury in vascular smooth muscle cells (VSMCs) in vitro. The effects of MAT2A, shMAT2A, shTRIM25 and/or Wnt inhibitor (IWR-1) on the viability, apoptosis and protein expressions of VSMCs were examined by CCK-8, Annexin V-FITC/PI and Western blot assays. In TAA mice, overexpression of MAT2A alleviated thoracic aortic injury, inhibited the aberrant expressions of aortic contractile proteins and dedifferentiation markers, and blocked the activation of Wnt/β-catenin pathway. In Ang-II-induced VSMCs, up-regulation of MAT2A increased cellular activity and repressed the expression of β-catenin protein. TRIM25 knockdown promoted activity of VSMCs, inhibited apoptosis, and blocked the Wnt/β-catenin pathway activation by binding to MAT2A. IWR-1 partially counteracted the regulatory effects of shMAT2A. Collectively, TRIM25 destabilises the mRNA of MAT2A to activate Wnt/β-catenin signaling and ultimately exacerbate TAA injury.
Continuous finite elements satisfying a strong discrete Miranda–Talenti identity
Authors: Gallistl Dietmar , Tian Shudan 
Published: September 2024

Abstract
This article introduces continuous $H^{2}$-nonconforming finite elements in two and three space dimensions that satisfy a strong discrete Miranda–Talenti inequality in the sense that the global $L^{2}$ norm of the piecewise Hessian is bounded by the $L^{2}$ norm of the piecewise Laplacian. The construction is based on globally continuous finite element functions with $C^{1}$ continuity on the vertices (2D) or edges (3D). As an application, these finite elements are used to approximate uniformly elliptic equations in nondivergence form under the Cordes condition without additional stabilization terms. For the biharmonic equation in three dimensions, the proposed methods has less degrees of freedom than existing nonconforming schemes of the same order. Numerical results in two and three dimensions confirm the practical feasibility of the proposed schemes.
A novel explicit optimized scheme for numerical simulation of elastic-wavefield separation
Authors: Guo Zixi , Huang Jiandong , Chen Dong , Chen Yiyu 
Published: September 2024

Abstract
Numerical simulation of elastic-wave equation helps us better understand the information of underground structures and elastic-wave imaging has attracted the widespread attention of researchers. Using elastic-wave imaging requires separating the compressional and shear wavefields. Therefore, we develop a novel explicit optimized scheme to simulate the separated elastic wavefield. We construct a kind of 1-norm objective function directly utilizing the dispersion error and employ the simulated annealing algorithm to acquire improved finite-difference operators, whose optimal coefficients can effectively suppress spatial numerical dispersion. Meanwhile, we introduce a rotated staggered-grid (RSG) approach to enhance computational stability. Then, our proposed scheme, called the optimized RSG approach, is applied to the elastic-wave equations and decoupled elastic-wave equations to simulate the decoupled compressional and shear wavefield propagation. Numerical dispersion analysis is consistent with numerical results. The waveform comparison shows that the optimized RSG approach possesses higher accuracy, and several complex models are used to validate the applicability and effectiveness of the presented scheme.
Working Vacations and Adventure: American Women Physician Volunteers to the Labrador Mission of Wilfred Grenfell Before 1914
Authors: Connor Jennifer J 
Published: September 2024

Abstract
Many accounts, autobiographical and scholarly, emphasize how volunteers portrayed their work in the mission established for fishers by British physician Wilfred Grenfell in Newfoundland and Labrador: as escapist adventure. Scholars have not studied women physicians or their motivations to volunteer, however. This oversight derives from their small number combined with lack of knowledge about this mission’s distinction from the foreign medical missions and domestic frontier missions that drew many women physicians to permanent positions. This study therefore discusses two American physicians, Alfreda B. Withington (1860-1951) and Emma E. Musson (1862-1913), who volunteered for summer service with this mission in 1907 and 1909, respectively. Through their publications, biographical sources, and clinical accounts, it reveals the appeal to them of such temporary, accessible volunteer service as a working vacation that rejuvenated. Importantly, it counters the skewed perspective of contemporary accounts in which the connection of Withington and Musson to an international celebrity, Wilfred Grenfell, overrode fuller considerations of their own lives, careers, and experiences. Finally, this examination suggests possible differences in their volunteerism between women physicians and their male counterparts: along with other women professionals, medical women often incorporated volunteer vacation experience into a continuum of similar endeavors in their careers.
Attenuation of HIV-specific T cell responses Among people with HIV on ART following dipyridamole treatment
Authors: Morris Benjamin C , Hixson Emily A , Klamar-Blain Cynthia , Gillespie Delbert G , Abebe Kaleab Z , Rinaldo Charles R , Mellors John W , Jackson Edwin K , Riddler Sharon A , Macatangay Bernard J C 
Published: September 2024

Abstract
Twelve weeks of dipyridamole increased extracellular adenosine levels and decreased T cell activation in people with human immunodeficiency virus (HIV). In this analysis, we investigated the effect of dipyridamole on HIV-specific T cell responses. We compared changes in Gag- and Env-specific T cell responses using intracellular cytokine staining, following 12 wk of dipyridamole treatment vs placebo. We evaluated whether frequencies of polyfunctional HIV-specific T cells were associated with purines in the adenosine pathway and with measures of HIV persistence and chronic inflammation. There was a significant decrease in CD4+ polyfunctional T cell responses to Gag (−62.6% vs −23.0%; P &lt; 0.001) and Env (−56.1% vs −6.0%; P &lt; 0.001) in the dipyridamole arm. In the dipyridamole group, lower frequencies of polyfunctional Env-specific CD4+ T cells were associated with higher plasma levels of adenosine (r = −0.85, P &lt; 0.01) and inosine (r = −0.70, P = 0.04). Higher adenosine levels induced by dipyridamole treatment is associated with decreased HIV-specific CD4+ T cell polyfunctional responses in people with HIV on antiretroviral therapy.