bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with high consumption of soft drinks among Australian secondary-school students
Authors: Scully Maree , Morley Belinda , Niven Philippa , Crawford David , Pratt Iain S , Wakefield Melanie 
Published: February 2017

AbstractObjectiveTo examine demographic and behavioural correlates of high consumption of soft drinks (non-alcoholic sugar-sweetened carbonated drinks excluding energy drinks) among Australian adolescents and to explore the associations between high consumption and soft drink perceptions and accessibility.DesignCross-sectional self-completion survey and height and weight measurements.SettingAustralian secondary schools.SubjectsStudents aged 12–17 years participating in the 2012–13 National Secondary Students’ Diet and Activity (NaSSDA) survey (n 7835).ResultsOverall, 14 % of students reported consuming four or more cups (≥1 litres) of soft drinks each week (‘high soft drink consumers’). Demographic factors associated with high soft drink consumption were being male and having at least $AU 40 in weekly spending money. Behavioural factors associated with high soft drink consumption were low fruit intake, consuming energy drinks on a weekly basis, eating fast foods at least once weekly, eating snack foods ≥14 times/week, watching television for >2 h/d and sleeping for <8 h/school night. Students who perceived soft drinks to be usually available in their home, convenient to buy and good value for money were more likely to be high soft drink consumers, as were students who reported usually buying these drinks when making a beverage purchase from the school canteen/vending machine.ConclusionsHigh soft drink consumption clusters with other unhealthy lifestyle behaviours among Australian secondary-school students. Interventions focused on reducing the availability of soft drinks (e.g. increased taxes, restricting their sale in schools) as well as improved education on their harms are needed to lower adolescents’ soft drink intake.
Regulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2
Authors: Perander Maria , Al-Mahdi Rania , Jensen Thomas C. , Nunn Jennifer A. L. , Kildalsen Hanne , Johansen Bjarne , Gabrielsen Mads , Keyse Stephen M. , Seternes Ole-Morten 
Published: March 2017

AbstractThe atypical MAP kinases ERK3 and ERK4 are activated by phosphorylation of a serine residue lying within the activation loop signature sequence S-E-G. However, the regulation of ERK3 and ERK4 phosphorylation and activity is poorly understood. Here we report that the inducible nuclear dual-specificity MAP kinase phosphatase (MKP) DUSP2, a known regulator of the ERK and p38 MAPKs, is unique amongst the MKP family in being able to bind to both ERK3 and ERK4. This interaction is mediated by a conserved common docking (CD) domain within the carboxyl-terminal domains of ERK3 and ERK4 and the conserved kinase interaction motif (KIM) located within the non-catalytic amino terminus of DUSP2. This interaction is direct and results in the dephosphorylation of ERK3 and ERK4 and the stabilization of DUSP2. In the case of ERK4 its ability to stabilize DUSP2 requires its kinase activity. Finally, we demonstrate that expression of DUSP2 inhibits ERK3 and ERK4-mediated activation of its downstream substrate MK5. We conclude that the activity of DUSP2 is not restricted to the classical MAPK pathways and that DUSP2 can also regulate the atypical ERK3/4-MK5 signalling pathway in mammalian cells.
A genetically encoded toolkit for tracking live-cell histidine dynamics in space and time
Authors: Hu Hanyang , Gu Yanfang , Xu Lei , Zou Yejun , Wang Aoxue , Tao Rongkun , Chen Xianjun , Zhao Yuzheng , Yang Yi 
Published: March 2017

AbstractHigh-resolution spatiotemporal imaging of histidine in single living mammalian cells faces technical challenges. Here, we developed a series of ratiometric, highly responsive, and single fluorescent protein-based histidine sensors of wide dynamic range. We used these sensors to quantify subcellular free-histidine concentrations in glucose-deprived cells and glucose-fed cells. Results showed that cytosolic free-histidine concentration was higher and more sensitive to the environment than free histidine in the mitochondria. Moreover, histidine was readily transported across the plasma membrane and mitochondrial inner membrane, which had almost similar transport rates and transport constants, and histidine transport was not influenced by cellular metabolic state. These sensors are potential tools for tracking histidine dynamics inside subcellular organelles, and they will open an avenue to explore complex histidine signaling.
Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin
Authors: Bonnet J. , Cartannaz J. , Tourcier G. , Contreras-Martel C. , Kleman J. P. , Morlot C. , Vernet T. , Di Guilmi A. M. 
Published: March 2017

AbstractUnusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 Å. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.
Effects of multiple genetic loci on the pathogenesis from serum urate to gout
Authors: Dong Zheng , Zhou Jingru , Jiang Shuai , Li Yuan , Zhao Dongbao , Yang Chengde , Ma Yanyun , Wang Yi , He Hongjun , Ji Hengdong , Yang Yajun , Wang Xiaofeng , Xu Xia , Pang Yafei , Zou Hejian , Jin Li , Wang Jiucun 
Published: March 2017

AbstractGout is a common arthritis resulting from increased serum urate, and many loci have been identified that are associated with serum urate and gout. However, their influence on the progression from elevated serum urate levels to gout is unclear. This study aims to explore systematically the effects of genetic variants on the pathogenesis in approximately 5,000 Chinese individuals. Six genes (PDZK1, GCKR, TRIM46, HNF4G, SLC17A1, LRRC16A) were determined to be associated with serum urate (PFDR < 0.05) in the Chinese population for the first time. ABCG2 and a novel gene, SLC17A4, contributed to the development of gout from hyperuricemia (OR = 1.56, PFDR = 3.68E-09; OR = 1.27, PFDR = 0.013, respectively). Also, HNF4G is a novel gene associated with susceptibility to gout (OR = 1.28, PFDR = 1.08E-03). In addition, A1CF and TRIM46 were identified as associated with gout in the Chinese population for the first time (PFDR < 0.05). The present study systematically determined genetic effects on the progression from elevated serum urate to gout and suggests that urate-associated genes functioning as urate transporters may play a specific role in the pathogenesis of gout. Furthermore, two novel gout-associated genes (HNF4G and SLC17A4) were identified.
Susceptibility profiles of Nocardia spp. to antimicrobial and antituberculotic agents detected by a microplate Alamar Blue assay
Authors: Zhao Pan , Zhang Xiujuan , Du Pengcheng , Li Guilian , Li Luxi , Li Zhenjun 
Published: March 2017

AbstractNocardia species are ubiquitous in natural environments and can cause nocardiosis. Trimethoprim-sulfamethoxazole has long been the monotherapy treatment of choice, but resistance to this treatment has recently emerged. In this study, we used microplate Alamar Blue assays to determine the antimicrobial susceptibility patterns of 65 standard Nocardia isolates, including 28 type strains and 20 clinical Nocardia isolates, to 32 antimicrobial agents, including 13 little studied drugs. Susceptibility to the most commonly used drug, trimethoprim-sulfamethoxazole, was observed in 98% of the isolates. Linezolid, meropenem, and amikacin were also highly effective, with 98%, 95%, and 90% susceptibility, respectively, among the isolates. The isolates showed a high percentage of resistance or nonsusceptibility to isoniazid, rifampicin, and ethambutol. For the remaining antimicrobials, resistance was species-specific among isolates and was observed in traditional drug pattern types. In addition, the antimicrobial susceptibility profiles of a variety of rarely encountered standard Nocardia species are reported, as are the results for rarely reported clinical antibiotics. We also provide a timely update of antimicrobial susceptibility patterns that includes three new drug pattern types. The data from this study provide information on antimicrobial activity against specific Nocardia species and yield important clues for the optimization of species-specific Nocardia therapies.
Atomically informed nonlocal semi-discrete variational Peierls-Nabarro model for planar core dislocations
Authors: Liu Guisen , Cheng Xi , Wang Jian , Chen Kaiguo , Shen Yao 
Published: March 2017

AbstractPrediction of Peierls stress associated with dislocation glide is of fundamental concern in understanding and designing the plasticity and mechanical properties of crystalline materials. Here, we develop a nonlocal semi-discrete variational Peierls-Nabarro (SVPN) model by incorporating the nonlocal atomic interactions into the semi-discrete variational Peierls framework. The nonlocal kernel is simplified by limiting the nonlocal atomic interaction in the nearest neighbor region, and the nonlocal coefficient is directly computed from the dislocation core structure. Our model is capable of accurately predicting the displacement profile, and the Peierls stress, of planar-extended core dislocations in face-centered cubic structures. Our model could be extended to study more complicated planar-extended core dislocations, such as <110> {111} dislocations in Al-based and Ti-based intermetallic compounds.
Correlation Characterization of Particles in Volume Based on Peak-to-Basement Ratio
Authors: Vovk Tatiana A. , Petrov Nikolay V. 
Published: March 2017

AbstractWe propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation function peak-to-basement ratio calculated for these images. After preliminary calibration via numerical simulation, the proposed method allows one to quickly distinguish parameters of the particle distribution and evaluate their concentration. The experimental verification was carried out for the two types of physical suspensions. Our method can be applied in environmental and biological research, which includes analyzing tools in flow cytometry devices, express characterization of particles and biological cells in air and water media, and various technical tasks, e.g. the study of scattering objects or rapid determination of cutting tool conditions in mechanisms.
Superluminal wave propagation in a non‐Foster negative capacitor loaded transmission line
Authors: Niang A. , de Lustrac A. , Burokur S.N. 
Published: March 2017

Superluminal wave propagation phenomenon achieved from a periodic structure in which a transmission line (TL) is loaded with non‐Foster negative capacitors is presented. The loading decreases the effective capacitance and the effective permittivity of the dielectric substrate of the TL. An experimental investigation of the superluminal phase and group velocity in the non‐Foster loaded TL is reported. The phase and group velocities are extracted from measured S‐parameters. To do so, three non‐Foster negative capacitors operating over [50–350 MHz] frequency band are integrated in a TL. Superluminal broadband effects are obtained from 50 to 220 MHz. The negative capacitors loaded TL paves the way to innovative broadband leaky wave antennas, active metamaterial surfaces and cloaking devices.
APMP supplementary comparison report of absorbed dose rate in tissue for beta radiation (BIPM KCDB: APMP.RI(I)-S2)
Authors: Kato M , Kurosawa T , Saito N , Kadni T B , Kim I J , Kim B C , Yi C -Y , Pungkun V , Chu C -H 
Published: March 2017

The supplementary comparison of absorbed dose rate in tissue for beta radiation (APMP.RI(I)-S2) was performed with five national metrology institutes in 2013 and 2014. Two commercial thin window ionization chambers were used as transfer instruments and circulated among the participants. Two of the NMIs measured the calibration coefficients of the chambers in reference fields produced from Pm-147, Kr-85 and Sr-90/Y-90, while the other three measured those only in Sr-90/Y-90 beta-particle field. The degree of equivalence for the participants was determined and this comparison verifies the calibration capabilities of the participating laboratories. In addition, most of the results of this comparison are consistent with another international comparison (EUROMET.RI(I)-S2) reported before this work.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Report of the CCQM-K123: trace elements in biodiesel fuel
Authors: Kuroiwa T , Zhu Y , Inagaki K , Long S E , Christopher S J , Puelles M , Borinsky M , Hatamleh N , Murby J , Merrick J , White I , Saxby D , Sena R C , Almeida M D , Vogl J , Phukphatthanachai P , Fung W H , Yau H P , Okumu T O , Kang'iri J N , Télle J A S , Campos E Z , Galv´n E C , Kaewkhomdee N , Taebunpakul S , Thiengmanee U , Yafa C , Tokman N , Tunç M , Can S Z 
Published: March 2017

The CCQM-K123 key comparison was organized by the Inorganic Analysis Working Group (IAWG) of CCQM to assess and document the capabilities of the national metrology institutes (NMIs) or the designated institutes (DIs) to measure the mass fractions of sodium, calcium, potassium, magnesium phosphorous and sulfur in biodiesel fuel (BDF). National Metrology Institute of Japan (NMIJ) and National Institute of Standards and Technology (NIST) acted as the coordinating laboratories.
Results were submitted by 11 NMIs and DIs. Most of the participants used inductively coupled plasma-mass spectrometry (ICP-MS), isotope dilution technique with ICP-MS and inductively coupled plasma-optical emission spectrometry (ICP-OES) with microwave acid digestion.
Accounting for relative expanded uncertainty, comparability of measurement results for each of Na, Ca, K, Mg and P was successfully demonstrated by the participants. Concerning S, the variation in results between participants, particularly those using IDMS methods was observed. According to the additional evaluation and investigation, the revised results were overlapping between IDMS measurements at the k = 2 level. However, this KC does not support S measurements.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Pilot Study EURAMET.AUV.V-P1: Bilateral comparison on magnitude of the complex charge sensitivity of accelerometers from 10 Hz to 10 kHz
Authors: Bartoli Claire , Hermawanto Denny 
Published: March 2017

The results of a Pilot Study EURAMET.AUV.V-P1 between LNE (France) and RCM-LIPI (Indonesia) are reported. This bilateral comparison of sinusoidal vibration was organized after the implementation of various improvements at RCM-LIPI following a previous (unpublished) comparison that had revealed discrepancies in their results at frequencies above 5 kHz.
The results of this Pilot Study, using the same accelerometers as the earlier comparison, demonstrate that the discrepancies at high frequencies have been resolved. For both the back-to-back and the single-ended accelerometers tested, the sensitivities of the RCM-LIPI and the LNE over the frequency range from 10 Hz to 10 kHz now agree within their declared uncertainties.

Main text

To reach the main text of this paper, click on Final Report.
The final report has been peer-reviewed and approved for publication by the CCAUV.
Ecological data from observer programmes underpin ecosystem-based fisheries management
Authors: Gilman Eric , Weijerman Mariska , Suuronen Petri 
Published: February 2017

AbstractData required from fisheries monitoring programmes substantially expand as management authorities transition to implement elements of ecosystem-based fisheries management (EBFM). EBFM extends conventional approaches of managing single fishery effects on individual stocks of target species by taking into account the effects, within a defined ecosystem, of local to regional fisheries on biodiversity, from genotypes to ecological communities. This includes accounting for fishery effects on evolutionary processes, associated and dependent species, habitats, trophic food web processes, and functionally linked systems. Despite seemingly insurmountable constraints, through examples, we demonstrate how data routinely collected in most observer programmes and how minor and inexpensive expansions of observer data fields and collection protocols supply ecological data underpinning EBFM. Observer data enable monitoring bycatch, including catch and mortality of endangered, threatened and protected species, and assessing the performance of bycatch management measures. They provide a subset of inputs for ecological risk assessments, including productivity–susceptibility analyses and multispecies and ecosystem models. Observer data are used to monitor fishery effects on habitat and to identify and protect benthic vulnerable marine ecosystems. They enable estimating collateral sources of fishing mortality. Data from observer programmes facilitate monitoring ecosystem pressure and state indicators. The examples demonstrate how even rudimentary fisheries management systems can meet the ecological data requirements of elements of EBFM.
Using Wikipedia to Predict Election Outcomes
Authors: Smith Benjamin K. , Gustafson Abel 
Published: February 2017

AbstractThis study seeks to improve election forecasting by supplementing polling data with online information-seeking behavior trends as an indicator of public opinion. Aggregate trends of demonstrations of interest or engagement have been shown to accurately predict behavior trends and reflect public opinion. Further, because traditional poll-based predictions are inherently undermined by self-reporting biases and the intention-behavior disconnect, we can expect that information-seeking trends on widely used social media—as an autonomous and unobtrusive indicator of relative levels of public opinion—can help correct for some of this error and explain unique, additional variance in election results. We advance the literature by using data from Wikipedia pageviews along with polling data in a synthesized model based on the results of the 2008, 2010, and 2012 US Senate general elections. Results show that Wikipedia pageviews data significantly add to the ability of poll- and fundamentals-based projections to predict election results up to 28 weeks prior to Election Day, and benefit predictions most at those early points, when poll-based predictions are weakest.
Releasing potential power of terpene synthases by a robust precursor supply platform
Authors: Bian Guangkai , Han Yichao , Hou Anwei , Yuan Yujie , Liu Xinhua , Deng Zixin , Liu Tiangang 
Published: February 2017

AbstractApproximately 76,000 discovered makes terpenoids the largest family of natural products in nature with widespread applications. The wide-spectrum of structural diversity of the terpenoids were largely due to the variable skeletons generated by terpene synthases. The number of terpene skeletons found in nature, however, were so much more than those conceivably generated from known terpene synthases and the limited characterized terpene synthases also make no chance for some useful terpenoids overproduction in microbe. Here, we first demonstrated that the promiscuous synthasesin vivocan produce more variable terpenoid products by converting precursors of different lengths (C10, C15, C20, C25). This discovery was prompted by the development of an efficientin vivoplatform by combining the two promiscuous terpene synthases and three prenyltransferases to generate 50 terpenoids, at least 3 ring systems of which were completely new. Furthermore, protein engineering was further integrated to enhance product diversity. Clearly, the work is expected to dramatically reshape the terpenoid research by widening the flexibility of the terpene synthases for the fresh discovery or creation of the new terpenoid compounds by skeleton reframing.
Laminar organization of encoding and memory reactivation in the parietal cortex
Authors: Wilber Aaron A. , Skelin Ivan , McNaughton Bruce L. 
Published: February 2017

Egocentric neural coding has been observed in parietal cortex (PC), but its topographical and laminar organization is not well characterized. We used multi-site recording to look for evidence of local clustering and laminar consistency of linear and angular velocity encoding in multi-neuronal spiking activity (MUA) and in the high-frequency (300-900 Hz) component of the local field potential (HF-LFP), believed to reflect local spiking activity. Rats were trained to run many trials on a large circular platform, either to LED-cued goal locations or as a spatial sequence from memory. Tuning to specific self-motion states was observed consistently, and exhibited distinct cortical depth-invariant coding properties. These patterns of collective local and laminar activation during behavior were reactivated in compressed form during post-experience sleep, and temporally coupled to hippocampal sharp wave ripples. Thus, PC neuron motion encoding is consistent across cortical laminae, and this consistency is maintained during memory reactivation.HighlightsParietal cortex MUA encodes specific movements coherently across laminae.This organizational scheme is maintained during subsequent memory reactivationMUA and HF-LFP showed similar self-motion tuning and memory reactivation dynamicsThis establishes the utility of MUA and HF-LFP for human memory reactivation studies
An alternative STAT signaling pathway acts in antiviral immunity inCaenorhabditis elegans
Authors: Tanguy Mélanie , Véron Louise , Stempor Przemyslaw , Ahringer Julie , Sarkies Peter , Miska Eric A. 
Published: February 2017

AbstractAcross metazoans, innate immunity is vital in defending organisms against viral infection. In mammals, antiviral innate immunity is orchestrated by interferon signaling, activating the STAT transcription factors downstream of the JAK kinases to induce expression of antiviral effector genes. In the nematodeC. elegans, which lacks the interferon system, the major antiviral response so far described is RNA interference but whether additional gene expression responses are employed is not known. Here we show that, despite the absence of both interferon and JAK, theC. elegansSTAT homologue STA-1 orchestrates antiviral immunity. Intriguingly, mutants lacking STA-1 show increased resistance to antiviral infection. Using gene expression analysis and chromatin immunoprecipitation we show that, in contrast to the mammalian pathway, STA-1 acts as a transcriptional repressor. Thus STA-1 might act to suppress a constitutive antiviral response in the absence of infection. Using a reverse genetic screen we identify the SID-3 as a kinase upstream of STA-1 in the response to infection. Together, our work identifies a novel STAT regulatory cascade controlling its activity in antiviral resistance, illustrating the complex evolutionary trajectory displayed by innate immune signaling pathways across metazoan organisms.