bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perception of Behavioral Contagion of Adolescent Suicide
Authors: Range Lillian M. , Goggin William C. , Steede Kevin K. 
Published: December 2015

ABSTRACTIn order to assess perceptions of behavioral contagion of suicide (what people thought a disturbed adolescent would do if the teen knew about a suicide in the community), and to assess actor‐observer differences in such perceptions, 142 college students were asked to view a videotaped vignette of a distressed high school student, and then to assess her potential for committing suicide, running away, entering therapy, or abusing alcohol. Subjects who were told that the teenager knew of two recent suicides in the community (contagion group) rated the young woman as more likely to commit suicide or run away than did the subjects who were not told of the suicides (noncontagion group). Subjects who were instructed to imagine that they were the teenager (actors) blamed situational factors, and in particular the teen's parents, more for her distress than did subjects who were instructed just to rate the teenager on the videotape (observers). Contagion/actors rated suicide as more likely than did any other group. Apparently, people believe that behavioral contagion occurs when a suicide is reported, and they especially perceive themselves to be influenced by such information.
Disgust predicts prejudice and discrimination toward individuals with obesity
Authors: Vartanian Lenny R. , Trewartha Tara , Vanman Eric J. 
Published: December 2015

AbstractThis study examined the relevance of disgust to evaluations of an obese target person, and the connection between disgust and prejudice toward that person. Participants (n = 598) viewed an image of an obese or non‐obese woman, and then evaluated that woman on a number of dimensions (emotions, attitudes, stereotypes, desire for social distance). Compared with the non‐obese target, the obese target elicited more disgust, more negative attitudes and stereotypes, and a greater desire for social distance. Furthermore, disgust mediated the effect of the target's body size on all of the outcome variables (attitudes, stereotypes, social distance). Disgust plays an important role in prejudice and discrimination toward individuals with obesity, and might in part explain the pervasiveness of weight bias.
Affective learning in adults with intellectual disability: an experiment using evaluative conditioning
Authors: Blanchette I. , Treillet V. , Davies S. R. 
Published: December 2015

AbstractBackgroundEvaluative conditioning is a form of affective learning in which initially neutral stimuli acquire an affective value through association with negative or positive stimuli. Recent research shows an important role for cognitive resources in this type of learning. This form of affective learning has rarely been studied in intellectual disability (ID).MethodWe examined evaluative conditioning in 16 adults with mild to moderate ID compared to age‐ and gender‐matched control participants. Neutral shapes and symbols were repeatedly paired with positive, neutral or negative unconditioned stimuli (faces or International Affective Picture System images). There was also an extinction phase.ResultsThere was significant acquisition of conditioning in both groups. Stimuli paired with positive images were evaluated more positively, and stimuli paired with negative images were evaluated more negatively. Post‐extinction ratings however show that these novel affective associations were not maintained by individuals with ID as much as by individuals in the control group.ConclusionsWe conclude that ID modulates some aspects of affective learning but not necessarily initial preference acquisition.
Cardiorespiratory fitness in 16 025 adults aged 18–91 years and associations with physical activity and sitting time
Authors: Eriksen L. , Grønbæk M. , Helge J. W. , Tolstrup J. S. 
Published: December 2015

Our aim was to provide up‐to‐date cardiorespiratory fitness reference data for adults of all ages and to investigate associations between cardiores‐piratory fitness and leisure time physical activity as well as sitting time. In the Danish Health Examination Survey 2007–2008, cardiorespiratory fitness was estimated in 16 025 individuals aged 18–91 years from validated cycle ergometer exercise tests. Level of leisure time physical activity (sedentary, light, moderate, and vigorous) and daily sitting time in hours was obtained from a self‐administered questionnaire. Men had 20–33% higher cardiorespiratory fitness than women, depending on age, and cardiorespiratory fitness decreased by 0.26 and 0.23 mL/min/kg per year in men and women, respectively. Cardiorespiratory fitness was higher among participants who reported a high level of physical activity in leisure time compared with participants who were sedentary. Among sedentary or lightly physically active participants, inverse associations between total daily sitting time and cardiorespiratory fitness were found, while there was no association between sitting time and cardiorespiratory fitness among moderately or vigorously physically active participants. These data on cardiorespiratory fitness can serve as useful reference material. Although reluctant to conclude on causality, sitting time might impact cardiorespiratory fitness among individuals with low levels of leisure time physical activity.
Diversification, evolution and methylation of short interspersed nuclear element families in sugar beet and related Amaranthaceae species
Authors: Schwichtenberg Katrin , Wenke Torsten , Zakrzewski Falk , Seibt Kathrin M. , Minoche André , Dohm Juliane C. , Weisshaar Bernd , Himmelbauer Heinz , Schmidt Thomas 
Published: December 2015

SummaryShort interspersed nuclear elements (SINEs) are non‐autonomous non‐long terminal repeat retrotransposons which are widely distributed in eukaryotic organisms. While SINEs have been intensively studied in animals, only limited information is available about plant SINEs. We analysed 22 SINE families from seven genomes of the Amaranthaceae family and identified 34 806 SINEs, including 19 549 full‐length copies. With the focus on sugar beet (Beta vulgaris), we performed a comparative analysis of the diversity, genomic and chromosomal organization and the methylation of SINEs to provide a detailed insight into the evolution and age of Amaranthaceae SINEs. The lengths of consensus sequences of SINEs range from 113 nucleotides (nt) up to 224 nt. The SINEs show dispersed distribution on all chromosomes but were found with higher incidence in subterminal euchromatic chromosome regions. The methylation of SINEs is increased compared with their flanking regions, and the strongest effect is visible for cytosines in the CHH context, indicating an involvement of asymmetric methylation in the silencing of SINEs.
Opportunities and challenges in liquid cell electron microscopy
Authors: Ross Frances M. 
Published: December 2015

Advances in seeing small things
Electron microscopes, particularly those with aberration correction, can view materials at the subnanometer scale. Additional improvements make it possible to obtain images at lower electron doses, thus minimizing the damage to the sample. However, for a number of materials, particularly those of biological origin, samples need to be imaged in solution. Ross reviews recent advances that have made it possible to do liquid cell electron microscopy, which opens up the possibility of studying problems such as the changes inside a battery during operation, the growth of crystals from solution, or biological molecules in their native state.

Science
, this issue p.
10.1126/science.aaa9886
Dissecting the Role of E2 Protein Domains in Alphavirus Pathogenicity
Authors: Weger-Lucarelli James , Aliota Matthew T. , Wlodarchak Nathan , Kamlangdee Attapon , Swanson Ryan , Osorio Jorge E. 
Published: December 2015

ABSTRACTAlphaviruses represent a diverse set of arboviruses, many of which are important pathogens. Chikungunya virus (CHIKV), an arthritis-inducing alphavirus, is the cause of a massive ongoing outbreak in the Caribbean and South America. In contrast to CHIKV, other related alphaviruses, such as Venezuelan equine encephalitis virus (VEEV) and Semliki Forest virus (SFV), can cause encephalitic disease. E2, the receptor binding protein, has been implicated as a determinant in cell tropism, host range, pathogenicity, and immunogenicity. Previous reports also have demonstrated that E2 contains residues important for host range expansions and monoclonal antibody binding; however, little is known about what role each protein domain (e.g., A, B, and C) of E2 plays on these factors. Therefore, we constructed chimeric cDNA clones between CHIKV and VEEV or SFV to probe the effect of each domain on pathogenicityin vitroandin vivo. CHIKV chimeras containing each of the domains of the E2 (ΔDomA, ΔDomB, and ΔDomC) from SFV, but not VEEV, were successfully rescued. Interestingly, while all chimeric viruses were attenuated compared to CHIKV in mice, ΔDomB virus showed similar rates of infection and dissemination inAedes aegyptimosquitoes, suggesting differing roles for the E2 protein in different hosts. In contrast to CHIKV; ΔDomB, and to a lesser extent ΔDomA, caused neuron degeneration and demyelination in mice infected intracranially, suggesting a shift toward a phenotype similar to SFV. Thus, chimeric CHIKV/SFV provide insights on the role the alphavirus E2 protein plays on pathogenesis.IMPORTANCEChikungunya virus (CHIKV) has caused large outbreaks of acute and chronic arthritis throughout Africa and Southeast Asia and has now become a massive public health threat in the Americas, causing an estimated 1.2 million human cases in just over a year. No approved vaccines or antivirals exist for human use against CHIKV or any other alphavirus. Despite the threat, little is known about the role the receptor binding protein (E2) plays on disease outcome in an infected host. To study this, our laboratory generated chimeric CHIKV containing corresponding regions of the Semliki Forest virus (SFV) E2 (domains A, B, and C) substituted into the CHIKV genome. Our results demonstrate that each domain of E2 likely plays a critical, but dissimilar role in the viral life cycle. Our experiments show that manipulation of E2 domains can be useful for studies on viral pathogenesis and potentially the production of vaccines and/or antivirals.
Deletion of Murid Herpesvirus 4 ORF63 Affects the Trafficking of Incoming Capsids toward the Nucleus
Authors: Latif Muhammad Bilal , Machiels Bénédicte , Xiao Xue , Mast Jan , Vanderplasschen Alain , Gillet Laurent 
Published: December 2015

ABSTRACT

Gammaherpesviruses are important human and animal pathogens. Despite the fact that they display the classical architecture of herpesviruses, the function of most of their structural proteins is still poorly defined. This is especially true for tegument proteins. Interestingly, a potential role in immune evasion has recently been proposed for the tegument protein encoded by Kaposi's sarcoma-associated herpesvirus open reading frame 63 (ORF63). To gain insight about the roles of ORF63 in the life cycle of a gammaherpesvirus, we generated null mutations in the ORF63 gene of murid herpesvirus 4 (MuHV-4). We showed that disruption of ORF63 was associated with a severe MuHV-4 growth deficit both
in vitro
and
in vivo
. The latter deficit was mainly associated with a defect of replication in the lung but did not affect the establishment of latency in the spleen. From a functional point of view, inhibition of caspase-1 or the inflammasome did not restore the growth of the ORF63-deficient mutant, suggesting that the observed deficit was not associated with the immune evasion mechanism identified previously. Moreover, this growth deficit was also not associated with a defect in virion egress from the infected cells. In contrast, it appeared that MuHV-4 ORF63-deficient mutants failed to address most of their capsids to the nucleus during entry into the host cell, suggesting that ORF63 plays a role in capsid movement. In the future, ORF63 could therefore be considered a target to block gammaherpesvirus infection at a very early stage of the infection.

IMPORTANCE
The important diseases caused by gammaherpesviruses in human and animal populations justify a better understanding of their life cycle. In particular, the role of most of their tegument proteins is still largely unknown. In this study, we used murid herpesvirus 4, a gammaherpesvirus infecting mice, to decipher the role of the protein encoded by the viral ORF63 gene. We showed that the absence of this protein is associated with a severe growth deficit both
in vitro
and
in vivo
that was mainly due to impaired migration of viral capsids toward the nucleus during entry. Together, our results provide new insights about the life cycle of gammaherpesviruses and could allow the development of new antiviral strategies aimed at blocking gammaherpesvirus infection at the very early stages.
Identifying research priorities in anaesthesia and perioperative care: final report of the joint National Institute of Academic Anaesthesia/James Lind Alliance Research Priority Setting Partnership
Authors: Boney Oliver , Bell Madeline , Bell Natalie , Conquest Ann , Cumbers Marion , Drake Sharon , Galsworthy Mike , Gath Jacqui , Grocott Michael P W , Harris Emma , Howell Simon , Ingold Anthony , Nathanson Michael H , Pinkney Thomas , Metcalf Leanne 
Published: December 2015

ObjectiveTo identify research priorities for Anaesthesia and Perioperative Medicine.DesignProspective surveys and consensus meetings guided by an independent adviser.SettingUK.Participants45 stakeholder organisations (25 professional, 20 patient/carer) affiliated as James Lind Alliance partners.OutcomesFirst ‘ideas-gathering’ survey:Free text research ideas and suggestions.Second ‘prioritisation’ survey:Shortlist of ‘summary’ research questions (derived from the first survey) ranked by respondents in order of priority.Final ‘top ten’:Agreed by consensus at a final prioritisation workshop.ResultsFirst survey:1420 suggestions received from 623 respondents (49% patients/public) were refined into a shortlist of 92 ‘summary’ questions.Second survey:1718 respondents each nominated up to 10 questions as research priorities.Top ten:The 25 highest-ranked questions advanced to the final workshop, where 23 stakeholders (13 professional, 10 patient/carer) agreed the 10 most important questions:▸ What can we do to stop patients developing chronic pain after surgery?▸ How can patient care around the time of emergency surgery be improved?▸ What long-term harm may result from anaesthesia, particularly following repeated anaesthetics?▸ What outcomes should we use to measure the ‘success’ of anaesthesia and perioperative care?▸ How can we improve recovery from surgery for elderly patients?▸ For which patients does regional anaesthesia give better outcomes than general anaesthesia?▸ What are the effects of anaesthesia on the developing brain?▸ Do enhanced recovery programmes improve short and long-term outcomes?▸ How can preoperative exercise or fitness training, including physiotherapy, improve outcomes after surgery?▸ How can we improve communication between the teams looking after patients throughout their surgical journey?ConclusionsAlmost 2000 stakeholders contributed their views regarding anaesthetic and perioperative research priorities. This is the largest example of patient and public involvement in shaping anaesthetic and perioperative research to date.
Interobserver agreement for the ATS/ERS/JRS/ALAT criteria for a UIP pattern on CT
Authors: Walsh Simon L F , Calandriello Lucio , Sverzellati Nicola , Wells Athol U , Hansell David M 
Published: December 2015

ObjectivesTo establish the level of observer variation for the current ATS/ERS/JRS/ALAT criteria for a diagnosis of usual interstitial pneumonia (UIP) on CT among a large group of thoracic radiologists of varying levels of experience.Materials and methods112 observers (96 of whom were thoracic radiologists) categorised CTs of 150 consecutive patients with fibrotic lung disease using the ATS/ERS/JRS/ALAT CT criteria for a UIP pattern (3 categories—UIP, possibly UIP and inconsistent with UIP). The presence of honeycombing, traction bronchiectasis and emphysema was also scored using a 3-point scale (definitely present, possibly present, absent). Observer agreement for the UIP categorisation and for the 3 CT patterns in the entire observer group and in subgroups stratified by observer experience, were evaluated.ResultsInterobserver agreement across the diagnosis category scores among the 112 observers was moderate, ranging from 0.48 (IQR 0.18) for general radiologists to 0.52 (IQR 0.20) for thoracic radiologists of 10–20 years’ experience. A binary score for UIP versus possible or inconsistent with UIP was examined. Observer agreement for this binary score was only moderate. No significant differences in agreement levels were identified when the CTs were stratified according to multidisciplinary team (MDT) diagnosis or patient age or when observers were categorised according to experience. Observer agreement for each of honeycombing, traction bronchiectasis and emphysema were 0.59±0.12, 0.42±0.15 and 0.43±0.18, respectively.ConclusionsInterobserver agreement for the current ATS/ERS/JRS/ALAT CT criteria for UIP is only moderate among thoracic radiologists, irrespective of their experience, and did not vary with patient age or the MDT diagnosis.
HEATR1 Negatively Regulates Akt to Help Sensitize Pancreatic Cancer Cells to Chemotherapy
Authors: Liu Tongzheng , Fang Yuan , Zhang Haoxing , Deng Min , Gao Bowen , Niu Nifang , Yu Jia , Lee SeungBaek , Kim JungJin , Qin Bo , Xie Fang , Evans Debra , Wang Liewei , Lou Wenhui , Lou Zhenkun 
Published: December 2015

Abstract
Elucidating mechanisms of chemoresistance is critical to improve cancer therapy, especially for the treatment of pancreatic ductal adenocarcinoma (PDAC). Genome-wide association studies have suggested the less studied gene HEAT repeat-containing protein 1 (HEATR1) as a possible determinant of cellular sensitivity to different chemotherapeutic drugs. In this study, we assessed this hypothesized link in PDAC, where HEATR1 expression is downregulated significantly. HEATR1 silencing in PDAC cells increased resistance to gemcitabine and other chemotherapeutics, where this effect was associated with increased AKT kinase phosphorylation at the Thr308 regulatory site. Mechanistically, HEATR1 enhanced cell responsiveness to gemcitabine by acting as a scaffold to facilitate interactions between AKT and the protein phosphatase PP2A, thereby promoting Thr308 dephosphorylation. Consistent with these findings, treatment with the AKT inhibitor triciribine sensitized HEATR1-depleted PDAC cells to gemcitabine, suggesting that this therapeutic combination may overcome gemcitabine resistance in patients with low HEATR1 expression. Clinically, we found that HEATR1 downregulation in PDAC patients was associated with increased AKT phosphorylation, poor response to tumor resection plus gemcitabine standard-of-care treatment, and shorter overall survival. Collectively, our findings establish HEATR1 as a novel regulator of AKT and a candidate predictive and prognostic indicator of drug responsiveness and outcome in PDAC patients. Cancer Res; 76(3); 572–81. ©2015 AACR.
Treatment of Triple-Negative Breast Cancer with TORC1/2 Inhibitors Sustains a Drug-Resistant and Notch-Dependent Cancer Stem Cell Population
Authors: Bhola Neil E. , Jansen Valerie M. , Koch James P. , Li Hua , Formisano Luigi , Williams Janice A. , Grandis Jennifer R. , Arteaga Carlos L. 
Published: December 2015

Abstract
Approximately 30% of triple-negative breast cancers (TNBC) harbor molecular alterations in PI3K/mTOR signaling, but therapeutic inhibition of this pathway has not been effective. We hypothesized that intrinsic resistance to TORC1/2 inhibition is driven by cancer stem cell (CSC)-like populations that could be targeted to enhance the antitumor action of these drugs. Therefore, we investigated the molecular mechanisms by which PI3K/mTOR inhibitors affect the stem-like properties of TNBC cells. Treatment of established TNBC cell lines with a PI3K/mTOR inhibitor or a TORC1/2 inhibitor increased the expression of CSC markers and mammosphere formation. A CSC-specific PCR array revealed that inhibition of TORC1/2 increased FGF1 and Notch1 expression. Notch1 activity was also induced in TNBC cells treated with TORC1/2 inhibitors and associated with increased mitochondrial metabolism and FGFR1 signaling. Notably, genetic and pharmacologic blockade of Notch1 abrogated the increase in CSC markers, mammosphere formation, and in vivo tumor-initiating capacity induced by TORC1/2 inhibition. These results suggest that targeting the FGFR–mitochondrial metabolism–Notch1 axis prevents resistance to TORC1/2 inhibitors by eradicating drug-resistant CSCs in TNBC, and may thus represent an attractive therapeutic strategy to improve drug responsiveness and efficacy. Cancer Res; 76(2); 440–52. ©2015 AACR.
Exploring the Feasibility of a Broad-Reach Physical Activity Behavior Change Intervention for Women Receiving Chemotherapy for Breast Cancer: A Randomized Trial
Authors: Vallance Jeff K. , Friedenreich Christine M. , Lavallee Celeste M. , Culos-Reed Nicole , Mackey John R. , Walley Barbara , Courneya Kerry S. 
Published: December 2015

Abstract
Background: Facilitating healthy levels of physical activity (PA) during chemotherapy is important for the psychosocial and physical health of breast cancer survivors. The primary objective of this feasibility study was to examine the effects of a broad-reach PA behavior change intervention among women with breast cancer receiving adjuvant chemotherapy.
Methods: Breast cancer patients receiving adjuvant chemotherapy (N = 95) were randomly assigned to receive a PA resource kit consisting of tailored print materials and a step pedometer (intervention) or a standard public health PA recommendation (standard recommendation). The primary outcome was daily pedometer steps. Secondary outcomes were self-reported light, moderate, and vigorous intensity PA, total moderate-to-vigorous PA, and sedentary time. Assessments were conducted before and after adjuvant chemotherapy.
Results: Attrition was 19% (17 of 95). Intervention patients wore their step pedometer for 85 days (range, 35–144 days; SD = 26.4) for a 95% adherence rate. Analyses of covariance suggested that the intervention was not statistically superior to standard recommendation for daily average pedometer steps (−771; 95% CI = −2024 to 482; P = 0.22), total MVPA minutes (−4; 95% CI = −62 to 570; P = 0.90), or sedentary time (+160; 95% CI = −186 to 506; P = 0.42).
Conclusion: This broach-reach and low intensive intervention was not more effective for promoting PA in breast cancer patients receiving chemotherapy than providing the standard public health guidelines for PA.
Impact: Achieving physical activity behavior change during adjuvant breast cancer chemotherapy may require some level of supervised physical activity or more intensive (e.g., face-to-face, supervised) interventions. Cancer Epidemiol Biomarkers Prev; 25(2); 391–8. ©2015 AACR.