bluuebunny/crossref_metadata_2025_split huggingface dataset:
HLA‐B27 testing: A journey from flow cytometry to molecular subtyping
Authors: Chheda Pratiksha , Warghade Sandeep , Mathias Jyothi , Dama Tavisha , Matkar Sunmeet , Shah Nilesh , Bendre Rajesh 
Published: January 2018

BackgroundDetermination of HLA‐B27 status plays an important role as adjuvant in suspected cases for diagnosis of Ankylosing Spondilytis (AS). Objectives of this study were to evaluate (i) flow cytometry method in comparison with DNA microarray for HLA‐B27 typing and (ii) EUROArray HLA‐B27 Direct assay for HLA‐B27 allele detection along with discrimination of AS/non‐AS subtypes in Indian population.MethodsA total of 7543 patients with a presumptive clinical diagnosis of AS were referred for screening of HLA‐B27. All samples were initially tested by flow cytometry, and based on its findings, 1560 samples were analyzed for the presence of HLA‐B27 allele by microarray technology. A subset of samples (n = 200) were further tested by DNA sequencing for identification of HLA‐B27 subtypes.ResultsScreening of HLA‐B27 by flow cytometry reported 1551 positive (20.56%) and 5556 negative (73.65%) cases. Remaining 436 (5.78%) samples were identified within equivocal zone. Of cases (n = 1560) analyzed by microarray method, 1333 (85.44%) and 227 (14.55%) were detected microarray positive and negative, respectively. DNA sequencing identified HLA‐B*27:07 as the predominant subtype among cases showing ex2 positivity by microarray method. Of 200 cases, 20 cases (14 of HLA‐B*07 and 6 of HLA‐B*37) of HLA‐B27 cross‐reactive subtypes were also identified.ConclusionWe recommend DNA typing as a complementary tool along with flow cytometry to accomplish successful HLA‐B27 phenotype determination. This is the first study among Indian population to evaluate efficacy of EUROArray to detect B27 allele and its potential to indicate the presence of nondisease‐associated alleles in Indian population.
Genetic analysis of interleukin 18 gene polymorphisms in alopecia areata
Authors: Celik Sumeyya Deniz , Ates Omer 
Published: January 2018

BackgroundAlopecia areata (AA), which appears as nonscarring hair shedding on any hair‐bearing area, is a common organ‐specific autoimmune condition. Cytokines have important roles in the development of AA. Interleukin (IL) 18 is a significant proinflammatory cytokine that was found higher in the patients with AA. We aimed to investigate whether the IL‐18 (rs187238 and rs1946518) single nucleotide polymorphisms (SNPs) may be associated with AA and/or clinical outcome of patients with AA in Turkish population.MethodsGenotyping of rs187238 and rs1946518 SNPs were detected using sequence‐specific primer‐polymerase chain reaction (SSP‐PCR) method in 200 patients with AA and 200 control subjects.ResultsThe genotype distribution of rs1946518 (−607C>A) SNP was found to be statistically significantly different among patients with AA and controls (P = .0008). Distribution of CC+CA genotypes and frequency of −607/allele C of rs1946518 SNP were higher in patients with AA (P = .001, P = .001, respectively). The genotype distribution of rs187238 (−137G>C) SNP was found to be statistically significantly different among patients with AA and control subjects (P = .0014). Distribution of GG genotype and frequency of −137/allele G of rs187238 SNP were higher in patients with AA (P = .0003, P = .001, respectively).ConclusionThe rs1946518 (−607C>A) and rs187238 (−137G>C) polymorphisms were found associated with alopecia areata disease. The study suggests that IL‐18 rs187238 and rs1946518 SNPs may be the cause of the AA susceptibility.
Wirkung von organischen Schadstoffen (PAK; PCB) und Schwermetallen in Rieselfeldböden auf Biomasseertrag und Schwermetalltransfer bei Roggen (Secale Cereale)
Authors: Dorn Jana , Metz Reinhart 
Published: January 2018

AbstractRoggen (Secale cereale) wurde im Gefäßversuch auf unterschiedlich stark belasteten Rieselfeldböden und künstlich stoffspezifisch angereichertem Boden geprüft. Dazu wurde ein niedrig belasteter Rieselfeldboden (Referenzboden R) sowohl einzeln mit 2.21′,5.5′ Tetrachlorbiphenyl, Benzo‐a‐pyren, Cadmium und Kupfer als auch mit Kombinationen dieser organischen Schadstoffe und Schwermetalle kontaminiert. Diese künstlichen Varianten standen einem anthropogen hochbelasteten Rieselfeldboden (Großbeeren GB) gegenüber. Der Roggen zeigte auf allen Cu‐Anrei‐cherungsvarianten typische Schadsymptome, die der Wirkung des in gleicher Höhe kombiniert belasteten Rieselfeldbodens sehr ähnlich waren. Dabei wirkte Cu als Einzelstoff teilweise stärker als die im hochbelasteten Rieselfeld vorliegenden Kombinationen. Das deutet auf eine gegenseitige Wechselwirkung oder eine synergistische Wirkung hin.
Raman spectroscopy and density functional theory study of energetically closely separated C2′‐endo and C3′‐endo pentose forms in purine nucleoside analogue drug‐gold conjugates
Authors: Ganbold Erdene‐Ochir , Nguyen Thanh Danh , Ly Nguyen Hoang , Joo Sang‐Woo , Li Linzi , Kim B. Moon 
Published: January 2018

AbstractWe performed a vibrational analysis of a cyclin‐dependent kinase inhibitor as an anticancer drug using Raman spectroscopy and quantum mechanical calculations. BMK‐Y101, a purine nucleoside analogue cyclin‐dependent kinase inhibitor, was found to adsorb on gold nanoparticles as evidenced by ultraviolet–visible absorption spectroscopy. Density functional theory (DFT) calculations were introduced to examine the energetic stabilities of the tautomeric amino and imino purine rings as well as C3′‐endo and C2′‐endo pentose forms for the possible binding geometries to 6‐atom gold clusters. DFT calculations predicted that the N9 binding mode of the C2′‐endo amino purine conformer would be the most stable in coordination with the gold atoms, despite very small energy differences of 0.026 kcal/mol to the second stable conformer of the N1 coordinated state. Surface‐enhanced Raman scattering (SERS) spectra were analyzed with appropriate vibrational assignments according to the DFT calculations and potential energy distribution analysis. The vibrational band at ~1,460 cm−1, which can be ascribed to the ν(N9–C4)(20%) + ν(N3–C4)(13%) + ν(C10–C7)(12%) + ν(C7–C8)(12%) + ν(N3–C2)(11%) mode for the N9‐coordination on Au6, appeared to be the most prominent in the SERS spectra, as predicted from the DFT calculations. BMK‐Y101 appeared to be detached from gold nanoparticles efficiently not in cell culture media but in hepatocarcinoma cells. Our findings indicate that quantum mechanical DFT calculations can be successfully implemented to interpret the SERS spectral features of the nucleoside drug on Au surfaces.
Vibrational spectra of the rare‐occurring complex hydrogen arsenate minerals pharmacolite, picropharmacolite, and vladimirite: Dominance of Raman over IR spectroscopy to discriminate arsenate and hydrogen arsenate units
Authors: Makreski Petre , Todorov Jovica , Makrievski Vasil , Pejov Ljupčo , Jovanovski Gligor 
Published: January 2018

AbstractThis work represents the first complete experimental and theoretical study of the infrared (IR) spectra of pharmacolite, Ca(AsO3OH)·2H2O; vladimirite, Ca4(AsO3OH)(AsO4)2·4H2O; and picropharmacolite, Ca4Mg(AsO3OH)2(AsO4)2·11H2O. IR spectra, collected at both room and liquid nitrogen temperature, have shown similar spectral view among minerals in the region of ν1 to ν4 modes, surprisingly not involving complexity in vladimirite and picropharmacolite spectral view due to the presence of both AsO3OH and AsO4 units compared to pharmacolite where only AsO3OH units appear in the structure. On the other hand, vladimirite and picropharmacolite Raman spectra in the corresponding ν1 to ν4 region (955–350 cm−1) exhibit existence of 3 sets of 2 bands (6 in total) completely reflecting the existence of 3 symmetrically nonequivalent AsO4 groups in the structures of vladimirite and picropharmacolite (of which one is protonated) and only one set of ν1 to ν4 bands from the structurally equivalent AsO3OH units in the pharmacolite structure. The liquid nitrogen temperature IR spectra enabled to infer important information regarding the OH vibrational bands related to the AsO3OH groups and to the water molecules. Essentially, all bands in the vibrational spectra were assigned and correlated with the findings for a plethora of structurally similar complex hydrogen arsenate minerals. To support the tentative assignment of bands in the vibrational spectra, quantum theoretical calculations were performed within the framework of density functional theory. On the basis of theoretical calculations, a few reassignments of bands appearing in the OH stretching region are proposed. X‐ray powder diffraction was used to confirm authenticity of the title systems.
Quantifying nitrate dynamics in a mesotrophic lake using triple oxygen isotopes as tracers
Authors: Tsunogai Urumu , Miyauchi Takanori , Ohyama Takuya , Komatsu Daisuke D. , Ito Masanori , Nakagawa Fumiko 
Published: January 2018

AbstractVertical distributions of both concentrations and stable isotopic compositions of nitrate, including the 17O‐excesses (Δ17O), were determined four times during 1 yr within the mesotrophic water column of Lake Biwa in Japan. By using both the deposition rate of atmospheric nitrate onto the entire surface of the lake and the influx/efflux of both atmospheric and remineralized nitrate via streams reported in the literature, we quantified the annual dynamics of nitrate (gross production rate of nitrate through nitrification and gross metabolic rate of nitrate through assimilation and denitrification), together with their seasonal variations, based on the Δ17O method. The results revealed that 642 ± 113 Mmol (Mmol = 106 mol) of the remineralized nitrate was supplied into the water column through nitrification in the lake on an annual basis, while 810 ± 120 Mmol of nitrate was metabolized in the lake through assimilation and denitrification. In addition, it turns out that nitrification was active, not only in the hypolimnion, but also in the epilimnion and upper thermocline in this lake. Furthermore, the total metabolic rates of nitrate varied seasonally, with the highest rates in summer and the lowest in winter. Because the difference between the annual metabolic rate of nitrate estimated based on the Δ17O method and the annual assimilation rate of nitrate estimated based on the traditional 15N incubation method was only 20%, we concluded that the Δ17O method reliably estimates the dynamics of nitrate in mesotrophic lakes.
Identification of fish source Vibrio alginolyticus and evaluation of its bacterial ghosts vaccine immune effects
Authors: Cao Ji , Zhang Jiajun , Ma Lin , Li Lin , Zhang Wenchang , Li Jinnian 
Published: January 2018

AbstractVibrio alginolyticus (V. alginolyticus) is a common pathogen for humans and marine aquatic animals. Vibriosis of marine aquatic animals, caused by V. alginolyticus, has become more prevalent globally in recent years. Hence, a safe and effective vaccine is urgently needed for the control of this disease. Here, the strain 16‐3 isolated from the large yellow croaker (Larimichthys crocea) suffered from canker was identified as V. alginolyticus based on morphological, biochemical, and 16S rDNA sequencing analysis. Then, recombinant temperature‐controlled lysis plasmid pBV220‐lysisE was electroporated into the strain 16‐3 to generate V. alginolyticus bacterial ghosts (VaBGs) by inducing lysis gene E expression, and the safety and immune effects of VaBGs were further investigated in mice and large yellow croaker. The results showed that VaBGs were as safe as formalin‐killed V. alginolyticus cells (FKC) to mice and fish. Compared with FKC and PBS groups, significant elevations of the serum agglutinating antibody titer, serum bactericidal activity, lymphocyte proliferative responses, and levels of four different cytokines (Th1 type: IL‐2, TNF‐α; Th2 type: IL‐4 and IL‐6) in serum were detected in the VaBGs group, indicating that a Th1/Th2‐mediated mixed immune response was elicited by the VaBGs. More importantly, after challenged with the parent strain 16‐3, VaBGs‐vaccinated mice and fish showed higher protection than FKC‐vaccinated mice, the relative percent of survival (RPS) being 60%, 66.7% and 40%, respectively. Taken together, this is the first demonstration that the newly constructed V. alginolyticus ghosts may be developed as a safe and effective vaccine against V. alginolyticus infection in aquaculture.
Untangling the complexity of limb‐girdle muscular dystrophies
Authors: Liewluck Teerin , Milone Margherita 
Published: January 2018

ABSTRACTThe limb‐girdle muscular dystrophies (LGMDs) are a group of genetically heterogeneous, autosomal inherited muscular dystrophies with a childhood to adult onset, manifesting with hip‐ and shoulder‐girdle muscle weakness. When the term LGMD was first conceptualized in 1954, it was thought to be a single entity. Currently, there are 8 autosomal dominant (LGMD1A–1H) and 26 autosomal recessive (LGMD2A–2Z) variants according to the Online Mendelian Inheritance in Man database. In addition, there are other genetically identified muscular dystrophies with an LGMD phenotype not yet classified as LGMD. This highlights the entanglement of LGMDs, which represents an area in continuous expansion. Herein we aim to simplify the complexity of LGMDs by subgrouping them on the basis of the underlying defective protein and impaired function. Muscle Nerve 58: 167–177, 2018
Alveolar soft part sarcoma in children and young adults: A report of 69 cases
Authors: Flores Ricardo J. , Harrison Douglas J. , Federman Noah C. , Furman Wayne L. , Huh Winston W. , Broaddus Emily G. , Okcu Mehmet F. , Venkatramani Rajkumar 
Published: January 2018

AbstractBackgroundAlveolar soft part sarcoma (ASPS) is a rare mesenchymal tumor characterized by ASPL‐TFE3 translocation. Apart from complete surgical resection, there is no standard management strategy.ProcedureThe clinical data of 69 children and young adults less than 30 years old with ASPS diagnosed from 1980–2014 were retrospectively collected from four major institutions.ResultsMedian age at diagnosis was 17 years (range: 1.5–30). Forty‐four (64%) were female. Median follow‐up was 46 months (range: 1–409). Most common primary sites were limbs (58%) and trunk (24%). ASPL‐TFE3 translocation was present in all 26 patients tested. IRS postsurgical staging was I in 19 (28%), II in 7 (10%), III in 5 (7%), and IV in 38 (55%) patients. The 5‐year event‐free survival (EFS) and overall survival (OS) were 38% and 72%, respectively. The 5‐year EFS and OS were 80% and 87%, respectively, for the 31 patients with localized tumors (IRS‐I‐II‐III), and 7% and 61%, respectively, for the 38 patients with metastatic tumors (IRS‐IV). Of 11 IRS‐IV patients who received targeted therapy upfront, two had partial response, six had stable disease, and three had progressive disease. Median time to progression for IRS‐IV patients was 12 months for those treated with targeted therapy, 7 months for cytotoxic chemotherapy (N = 15), and 4 months for observation only (N = 6).ConclusionLocalized ASPS has a good prognosis after gross total resection. ASPS is resistant to cytotoxic chemotherapy. Although there are no curative therapies for patients with metastatic disease, prolonged disease stabilization may be achieved with targeted therapies.
Patterns of Internet‐based health information seeking in adult survivors of childhood cancer
Authors: Claridy Mechelle D. , Hudson Melissa M. , Caplan Lee , Mitby Pauline A. , Leisenring Wendy , Smith Selina A. , Robison Leslie L. , Mertens Ann C. 
Published: January 2018

AbstractBackgroundTo assess where, when, and why survivors of childhood cancer seek health information.ProcedureData from the Childhood Cancer Survivor Study (CCSS) cohort (n = 1386) and Health Information National Trends Survey (n = 2385) were analyzed to determine the health information seeking strategies of childhood cancer survivors. Descriptive frequencies, χ2 analyses, t‐tests, and multivariable logistic regression models were used.ResultsTo seek health‐related information for themselves, 54% (n = 742) of the childhood survivors reported using the Internet in the past 12 months, compared to 45% of the general population (adjusted OR: 2.76; 95% CI: 2.40–3.19). Childhood cancer survivors who used the Internet for health information were more likely to be female, between the ages of 18–34, have received some college education or be a college graduate, and report being in poor health. Although survivors were less likely than the general population to trust health information from the Internet (P < 0.01), they indicated that they would like a secure website that uses information from their medical records to provide individualized health‐related information.ConclusionThe use of the Internet to access health information among the childhood cancer survivors was over 50%. Information on late effects was a high priority for most survivors, as was their interest in websites related to late effects and a website on patient information tailored to personal situations. Identification of factors associated with searching the Internet for cancer information may provide direction for development of effective cancer communication interventions for this at‐risk population.
A prospective study of family predictors of health‐related quality of life in pediatric brain tumor survivors
Authors: Quast Lauren F. , Phillips Peter C. , Li Yimei , Kazak Anne E. , Barakat Lamia P. , Hocking Matthew C. 
Published: January 2018

AbstractBackgroundThe objective of this study was to examine prospectively the associations between family functioning at the end of tumor‐directed treatment and the health‐related quality of life (HRQL) of pediatric brain tumor survivors (PBTSs) approximately 9 months later.ProcedureThirty‐five PBTS (age 6–16 years) and their mothers completed measures of family functioning and survivor HRQL within 5 months of completing tumor‐directed therapy (baseline) and again approximately 9 months later (follow‐up).ResultsSurvivor‐rated general family functioning at baseline significantly predicted mother proxy‐ and self‐reported survivor HRQL at follow‐up when controlling for survivor HRQL at baseline and relevant demographic and treatment‐related variables.ConclusionsFamily functioning is a key factor contributing to survivor HRQL and should be screened throughout the course of tumor‐directed treatment. Psychosocial interventions directed toward improving general family functioning may improve survivor well‐being following the completion of treatment.
Robotic‐assisted coronary artery bypass surgery: an 18‐year single‐centre experience
Authors: Giambruno Vincenzo , Chu Michael W. , Fox Stephanie , Swinamer Stuart A. , Rayman Reiza , Markova Zarina , Barnfield Rebecca , Cooper Mitchell , Boyd Douglas W. , Menkis Alan , Kiaii Bob 
Published: January 2018

AbstractBackgroundMinimally invasive robot‐assisted direct coronary artery bypass (RADCAB) has emerged as a feasible minimally invasive surgical technique for revascularization that might offer several potential advantages over conventional approaches. We present our 18‐year experience in RADCAB.MethodsBetween February 1998 and February 2016, 605 patients underwent RADCAB. Patients underwent post‐procedural selective graft patency assessment using cardiac catheterization.ResultsThe mortality rate was 0.3%. The rate of conversion to sternotomy for any cause was reduced from 16.0% of the first 200 cases to 6.9% of the last 405 patients. The patency rate of the LITA‐to‐LAD anastomosis was 97.4%. Surgical re‐exploration for bleeding occurred in 1.8% of patients, and the transfusion rate was 9.2%. Average ICU stay was 1.2 ± 1.4 days, and average hospital stay was 4.8 ± 2.9 days.ConclusionsRobot‐assisted coronary artery bypass grafting is safe, feasible and it seems to represent an effective alternative to traditional coronary artery bypass grafting in selected patients.
A 3D Microporous MOF with mab Topology for Selective CO2 Adsorption and Separation
Authors: Pal Arun , Lin Jian‐Bin , Chand Santanu , Das Madhab C. 
Published: January 2018

AbstractA three dimensional MOF, {[Zn(OBA)(L)0.5].DMA}n (IITKGP‐7, IITKGP stands for Indian Institute of Technology Kharagpur), has been synthesized by the combination of a V‐shaped organic acid linker (H2OBA=4, 4′‐oxybisbenzoic acid) with flexible N,N‐donor spacer (L=2,5‐bis(3‐pyridyl)‐3,4 – diaza‐2,4‐hexadiene), forming a network with mab topology (DMA=N, N‐dimethylacetamide). This MOF is characterized by Fourier‐transform infrared spectroscopy (FTIR), elemental analysis (EA), powder X‐ray diffraction (PXRD), thermo gravimetric analysis (TGA) and single crystal X‐ray diffraction. The framework exhibits rhombus‐shaped channels of approximate size of 5.3 Å x 6.4 Å along crystallographic b axis with a potential solvent accessible volume of 31.8%. Gas sorption measurements of the evacuated framework shows preferential uptake of CO2 at 273 and 295 K under 1 bar over N2 and CH4. Calculations based on ideal adsorbed solution theory (IAST) show that the selectivity values of CO2/N2 (15:85) are 57 at 273 K and 121.4 at 295 K, whereas CO2/CH4 (50:50) selectivity values are 8.4 at 273 K and 8.8 at 295 K under 1 bar. The high CO2 separation selectivity over N2 and CH4 makes this MOF a potential candidate for CO2 separation from flue gas mixture and landfill gas mixture.
Solution Processable 1D Fullerene C60 Crystals for Visible Spectrum Photodetectors
Authors: Saran Rinku , Curry Richard J. 
Published: January 2018

AbstractVisible spectrum photodetector devices fabricated using molecular crystals of carbon C60 are reported. The devices operate efficiently, extending over and beyond the full visible light spectrum (300–710 nm) with a bias voltage tunable responsivity of 4 mA–0.5 mA W−1. Across this range of wavelengths, the noise equivalent power of these devices remains below 102 nW Hz−1/2, providing a detectivity of 107 Jones. The noise current in these devices is found to have a strong dependence on both bias voltage and frequency, varying by 4 orders of magnitude from 1 nA Hz−1/2 to 0.1 pA Hz−1/2. The devices also display a near‐linear dependence of photocurrent on light intensity over 4 orders of magnitude, providing a dynamic range approaching 80 dB. The 3 dB bandwidth of the devices is found to be above 102 Hz, while the 18 dB bandwidth exceeds 1 kHz. The transient photocurrents of the devices have a rise time of ≈50 µs and a long fall time of ≈4 ms. The spectral photocurrent of the devices is found to quench gradually with a reduction in temperature from ≈300 K and is fully quenched at temperatures below T ≈ 100 K. Upon reheating, the device performance is fully recovered.
The developmental trinity of mind: Cognizance, executive control, and reasoning
Authors: Demetriou Andreas , Makris Nikolaos , Kazi Smaragda , Spanoudis George , Shayer Michael 
Published: January 2018

This paper summarizes research on how cognizance, that is, awareness of mental processes, interacts with executive control and reasoning from childhood to adolescence. Central positions are that (a) cognizance changes extensively with age; (b) it contributes to the formation of executive control, and (c) mediates between executive control and reasoning. Cognizance recycles with changes in executive and inferential possibilities in four developmental cycles: it registers their present state, yielding insight into their operation, allowing their better management; this catalyzes their transformation into the next level. Implications for theory of intellectual development and practical implications for education are discussed.This article is categorized under:Psychology > Development and AgingNeuroscience > CognitionNeuroscience > DevelopmentPhilosophy > Consciousness