bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Utility of Circulating Tumor DNA for Detecting Lung Cancer Mutations by Targeted Next‐Generation Sequencing With Insufficient Tumor Samples
Authors: Sun Yi , Zhang Xu , Yang Xinhua , Ma Jiangjun 
Published: September 2024

ABSTRACTBackgroundCirculating tumor deoxyribonucleic acid (ctDNA) is increasingly applied in clinical practice. This study aimed to explore clinical utility of a minimal invasive and sensitive way of ctDNA for next‐generation sequencing in non–small cell lung cancer (NSCLC) with inadequate tumor samples.MethodsTargeted DNA sequencing was performed on tissue biopsies and matched plasma samples from 60 patients with NSCLC.ResultsA total of 13 driving genes were detected in 60 matched tissue DNA (tDNA) and ctDNA samples. Overall concordance rate was 75.47%, with 77.55% sensitivity and 50% specificity. Epidermal growth factor receptor (EGFR) mutations were the most common in both tDNA and ctDNA samples. Among other mutated genes were tumor protein p53 (TP53), erb‐b2 receptor tyrosine kinase 2 (ERBB2), anaplastic lymphoma kinase (ALK), cyclin‐dependent kinase inhibitor 2A (CDKN2A), ros proto‐oncogene 1, and receptor tyrosine kinase (ROS1). Mutations in b‐raf proto‐oncogene, serine/threonine kinase (BRAF), cluster of differentiation 274 (CD274), neurotrophin receptor tyrosine kinase 1 (NTRK1), and rearranged during transfection (RET) occurred only in plasma. The majority of mutations in both samples were single‐nucleotide variants. Deletions were found in EGFR, BRAF, and TP53 in ctDNA, whereas in tDNA, deletions were only found in EGFR. In ALK, single nucleic acid‐site amplification occurred simultaneously in tissue and plasma, but insertions and copy number variations were detected only in plasma.ConclusionsIdentifying ctDNA mutations by targeted sequencing in plasma is feasible, showing the clinical value of ctDNA‐targeted sequencing in NSCLC patients when tumor tissue sampling is insufficient or even impossible.
Role of ultrasound in diagnosing gravid uterine incarceration
Authors: Dong Yanhua , Liu Mengyuan , Dong Xiaozhen , Li Jie , Li Hezhou 
Published: September 2024

AbstractObjectiveTo summarize the ultrasound characteristics, diagnostic experiences, and pregnancy outcomes of gravid uterine incarceration.MethodsA retrospective analysis was conducted on the data of pregnant women diagnosed with gravid uterine incarceration by prenatal ultrasound at the Ultrasound Department of the Third Affiliated Hospital of Zhengzhou University from January 2020 to December 2023. Clinical data, ultrasound features, and pregnancy outcomes were analyzed.ResultsIn this study, a total of 23 pregnant women were included. Of these, eight were diagnosed in early pregnancy, and 15 were diagnosed in mid‐pregnancy. Seven participants had concurrent uterine fibroids, 10 had a history of abdominal or pelvic surgery, and two had ovarian cysts. A total of 13 cases presented with symptoms of urethral obstruction, three with rectal pressure symptoms, five cases with tight and stiff lower abdomen and two cases without special discomfort. Seventeen cases exhibited cervical compression with thinning and elongation, measuring approximately 39 to 73 mm. All 23 cases underwent manual or knee‐chest positioning repositioning, with one case requiring surgical intervention. Ultimately, 22 cases resulted in full‐term live births, one case experienced fetal demise at 24 weeks and one case experienced gravid uterine incarceration again in the third week after successful manual reduction, and manual reduction was performed again.ConclusionEarly diagnosis is critical for obstetric management and clinical prognosis, facilitating the successful release of the incarcerated uterus. The earlier the diagnosis, the higher the likelihood of successfully releasing the incarcerated uterus.
Extracellular vesicles derived from melanoma cells induce carcinoma‐associated fibroblasts via miR‐92b‐3p mediated downregulation of PTEN
Authors: Kewitz‐Hempel Stefanie , Windisch Nicola , Hause Gerd , Müller Lutz , Sunderkötter Cord , Gerloff Dennis 
Published: September 2024

AbstractIn melanoma, carcinoma‐associated fibroblasts (CAFs) are important cellular components in the tumour microenvironment due to their potential to promote tumour growth and metastatic spread of malignant cells. Melanoma cells have the ability to affect non‐tumour cells in the microenvironment by releasing extracellular vesicles (EVs). The mechanisms responsible for reprogramming normal dermal fibroblasts (NHDFs) into CAFs remain incompletely understood. However, it is likely thought to be mediated by melanoma‐specific miRNAs, which are transported by EVs derived from melanoma cells. Therefore, we wondered if one of the most enriched miRNAs in EVs secreted by melanoma cells, miR‐92b‐3p, is involved in the conversion of normal fibroblasts into CAFs. We observed that melanoma cell‐derived EVs indeed delivered miR‐92b‐3p into NHDFs and that its accumulation correlated with CAF formation, as demonstrated by enhanced expression of CAF marker genes and increased proliferation and migration. Overexpression of miR‐92b‐3p in NHDFs revealed similar results, while EVs deficient of miR‐92b‐3p did not induce a CAF phenotype. As a target we identified PTEN, whose repression led to increased expression of CAF markers. We thus provide a novel pathway of intercellular communication by which melanoma cells control the transformation of CAFs by virtue of EV‐transported miRNAs.
Single‐centre experience of implementing physiotherapist‐led prehabilitation for chimeric antigen receptor T cell therapy
Authors: McCourt Orla , Maciocia Paul , Roddie Claire , Hwang Angela , Wood Leigh , Panopoulou Aikaterini , Springell Deborah Ann , Al Bakir Maise , O'Reilly Maeve 
Published: September 2024

AbstractIntroductionThis report outlines the evaluation of physiotherapist‐led prehabilitation/rehabilitation for recipients of chimeric antigen receptor T (CAR‐T) cell therapy.MethodsA hybrid approach was used, incorporating in‐person assessment of quality of life and functional capacity (6‐min walk test and timed sit‐to‐stand test), and a personalised home exercise programme with remotely delivered physiotherapist support pre/post‐admission.ResultsFunctional deficits were prevalent at referral for CAR‐T. Prehabilitation and rehabilitation were highly acceptable to patients, and improvements in functional capacity were documented pre‐admission.ConclusionThis data highlights the importance of pre‐CAR‐T functional assessment and prehabilitation to optimise preparation and recovery.
Activation of the mPFC‐NAc Pathway Reduces Motor Impulsivity but Does Not Affect Risk‐Related Decision‐Making in Innately High‐Impulsive Male Rats
Authors: Arrondeau Chloé , Urueña‐Méndez Ginna , Marchessaux Florian , Goutaudier Raphaël , Ginovart Nathalie 
Published: September 2024

ABSTRACTAttention‐deficit/hyperactivity disorder (ADHD) and substance use disorders (SUD) are characterized by exacerbated motor and risk‐related impulsivities, which are associated with decreased cortical activity. In rodents, the medial prefrontal cortex (mPFC) and nucleus accumbens (NAc) have been separately implicated in impulsive behaviors, but studies on the specific role of the mPFC‐NAc pathway in these behaviors are limited. Here, we investigated whether heightened impulsive behaviors are associated with reduced mPFC activity in rodents and determined the involvement of the mPFC‐NAc pathway in motor and risk‐related impulsivities. We used the Roman High‐ (RHA) and Low‐Avoidance (RLA) rat lines, which display divergent phenotypes in impulsivity. To investigate alterations in cortical activity in relation to impulsivity, regional brain glucose metabolism was measured using positron emission tomography and [18F]‐fluorodeoxyglucose ([18F]FDG). Using chemogenetics, the activity of the mPFC‐NAc pathway was either selectively activated in high‐impulsive RHA rats or inhibited in low‐impulsive RLA rats, and the effects of these manipulations on motor and risk‐related impulsivity were concurrently assessed using the rat gambling task. We showed that basal [18F]FDG uptake was lower in the mPFC and NAc of RHA compared to RLA rats. Activation of the mPFC‐NAc pathway in RHA rats reduced motor impulsivity, without affecting risk‐related decision‐making. Conversely, inhibition of the mPFC‐NAc pathway had no effect in RLA rats. Our results suggest that the mPFC‐NAc pathway controls motor impulsivity, but has limited involvement in risk‐related decision‐making in our current model. Our findings suggest that reducing fronto‐striatal activity may help attenuate motor impulsivity in patients with impulse control dysregulation.
Criminal Genius or Everyday Villain? A Comparison of Malevolent Creativity Among Prisoners, Police Officers, and the General Population
Authors: Perchtold‐Stefan Corinna M. , Szabó Enikő , Rominger Christian , Fink Andreas , Opris Laura , Pataky Nóra 
Published: September 2024

ABSTRACTCriminals allegedly use effective novelty to intentionally exploit and harm others (creative fraud, theft, and murder). However, empirical evidence that criminals possess higher malevolent creativity than individuals without criminal backgrounds is lacking. We compared a male sample of prisoners in a maximum‐security penitentiary (n = 140), police officers (n = 122), and the general population (n = 106) on three different aspects of malevolent creativity: self‐reported malevolent creativity behavior (MCBS), willingness to engage in malevolent creativity on a test (MCT), and malevolent creativity potential on that test (reduced n = 285). Group comparisons (ANOVAs) differed for different malevolent creativity aspects: Prisoners reported more malevolent creativity behavior in daily life (MCBS) than nonprisoners, which may reflect their alleged criminal personality or the effects of confinement on creative coping with threat. However, prisoners also performed worse than police officers in generating creative ideas for taking revenge on others (MCT). No differences in initial willingness to engage in malevolent creativity (MCT) emerged. This discrepancy of self‐report and ability is discussed from several angles, including suitability of the applied measures and heterogeneity of prison populations. This study constitutes the first empirical insights into the often hypothesized but rarely tested malevolent creativity expression in the criminal mind.
Job Demands, Moral Disengagement, and Employee Behavior During the Pandemic: The Role of Family–Work Enrichment Among Healthcare Workers
Authors: Siddiqui Eram Fatima , Ghosh Koustab , Sharma Dheeraj 
Published: September 2024

ABSTRACTDuring crises like pandemics, organizations often face substantial disruptions that significantly increase job demands (JDs) on employees. This study investigates the impact of high JDs on moral disengagement (MD) and subsequent employee behaviors. Drawing upon social cognitive theory, we hypothesize that excessive JDs undermine self‐regulatory mechanisms, resulting in MD and affecting counterproductive work behavior (CWB) and extra‐role behaviors (ERBs). Additionally, we employ the conservation of resources theory to examine how family–work enrichment (FWE) can encourage ERB, potentially mitigating negative outcomes. To test our hypotheses, a two‐wave time‐lagged survey was conducted among hospital staff members during the COVID‐19 pandemic. The findings support our theoretical model, demonstrating that high JDs deactivate self‐regulatory systems, leading to MD and varied behavioral outcomes. However, FWE fosters ERB even among morally disengaged employees. This study advances the literature by elucidating the mechanisms of MD and highlighting the role of family support in promoting positive behaviors during crises. Implications for both theory and practice are discussed, offering insights for managing employee well‐being and performance in challenging times.
Data Analytics Capability Transforms Risk Management and Firm Performance
Authors: Fallahieh Moloud Soltanian , Mohezar Suhana , Kanapathy Kanagi 
Published: September 2024

ABSTRACTIn the contemporary globalized business environment, the escalating complexity in supply chains has led organizations to face an array of burgeoning risk factors. To address these challenges, firms strive to enhance their visibility across various supply chain levels by equipping themselves with the ability to scrutinize operational activities and effectively manage supply chain risks. This study employed a quantitative approach by administering a survey questionnaire to 158 manufacturing companies in Malaysia. Partial least squares structural equation modeling (PLS‐SEM) was utilized to examine the anticipated relationships. By drawing on organizational information processing theory principles, this study investigates the influence of supply chain operational reference (SCOR)—based data analytics capability (SCOR‐DAC) in bolstering firm performance by developing a more secure, risk‐averse enterprise, and improved strategic alignment. Additionally, this study investigates the mediating role of supply chain and risk management performance in the relationship between SCOR‐driven data analytics and organizational performance and the interaction between business strategy alignment and SCOR‐DAC. The study accentuates the notion that the capacity to manage disruptions, attained through improved risk management performance, positively impacts a firm's performance. Furthermore, the research underscores the importance of the synergy between data analytics and organizational strategies in constructing a holistic approach to risk management and performance enhancement. The findings offer valuable insights for companies aiming to enhance risk management and improve overall performance by increasing investments in data analytics and fostering a data‐driven culture for consistent business growth.
Choice of Adjuvant Radiotherapy Facility in Sinonasal Squamous Cell Carcinoma
Authors: Kaki Praneet C. , Patel Aman M. , Maxwell Russell , Brant Jason A. , Brody Robert M. , Adappa Nithin D. , Palmer James N. , Douglas Jennifer E. , Carey Ryan M. 
Published: September 2024

ObjectiveUndergoing surgery and adjuvant radiotherapy (aRT) at the same facility has been associated with higher overall survival (OS) in head and neck squamous cell carcinoma. Our study investigates whether undergoing surgery and aRT at the same academic facility is associated with higher OS compared with separate facilities in sinonasal squamous cell carcinoma (SNSCC).MethodsThe 2006 to 2017 National Cancer Database was queried for patients with SNSCC undergoing surgery at an academic facility followed by aRT with or without adjuvant chemotherapy. Multivariable binary logistic and Cox proportional hazards regression models were implemented.ResultsOf 419 patients satisfying inclusion criteria, 299 (71.4%) underwent surgery and aRT at the same academic facility. Residence in a less populated area (adjusted odds ratio [aOR] 1.75, 95% confidence interval [CI] 1.02–2.99, p = 0.042) and surgical facility case volume (aOR 2.51, 95% CI 1.21–5.21, p = 0.014) were associated with undergoing surgery and aRT at different facilities on multivariable logistic regression adjusting for patient demographics, clinicopathologic features, and adjuvant therapy (p < 0.05). Five‐year OS was higher among patients undergoing surgery and aRT at the same academic facility (64% vs. 55%, p = 0.039). Undergoing surgery and aRT at different facilities remained associated with worse OS on multivariable Cox regression (aHR 1.90, 95% CI 1.09–3.32, p = 0.023).ConclusionUndergoing surgery and aRT at the same academic facility is associated with higher OS in SNSCC. Academic physicians should carefully consider the recommendation of aRT treatment facility based on the level of benefit that the patient may derive from coordinated multidisciplinary care.Level of Evidence3 Laryngoscope, 135:705–715, 2025
Neurophysiological Assessment in Children with Vocal Fold Paralysis: A Tertiary Center Experience
Authors: D'Agostino Roberto , Cacco Tommaso , Fiz Ivana , Della Rocca Mirta , Martelli Simona , Cataldi Matteo , Lanteri Paola 
Published: September 2024

ObjectiveThe aim of this study is to report on experience acquired during the laryngeal electrophysiological assessment with Co‐MEP and L‐EMG in pediatric patients with acquired, congenital, and syndromic vocal fold paralysis (VFP), and correlate our findings with patients' characteristics, their comorbidities, and VFP etiology.MethodsPediatric patients with suspected or previously diagnosed unilateral or bilateral VFP underwent electrophysiological records under general anesthesia; corticobulbar motor‐evoked potentials (Co‐MEPs) and laryngeal electromyography (L‐EMG) of thyroarytenoid (TA) and posterior cricoarytenoid (PCA) muscles were recorded.ResultsStatistical analysis revealed a statistically significant correlation between early gestational age at childbirth and TA muscle intensity (p = 0.002) and PCA muscle intensity (p = 0.002); tracheostomy presence and TA muscle intensity (p = 0.002) and PCA muscle intensity (p = 0.002); presence of genetic anomalies with intensity and latency for TA muscle and latency for PCA muscle (TA latency p = 0.015, TA intensity p = 0.021, PCA latency p = 0.035); congenital presentation of VFP and an increased intensity for TA muscle (p = 0.04); latency and intensity for TA muscle (p = 0.024); TA muscle intensity and PCA intensity (p = 0.005).ConclusionIntraoperative Co‐MEPs and L‐EMG are two complementary tools for evaluating the functional integrity of the structures involved in conveying signals from the motor cortex to TA and PCA muscles in children with vocal fold paralysis. Further studies are needed to establish their ability to predict the recovery of VF mobility, which could potentially lead to decannulation.Level of Evidence4 Laryngoscope, 135:935–941, 2025
Broadband Unidirectional Thermal Emission
Authors: Ma Yue , Wang Jiawei , Li Longnan , Liu Tianji , Li Wei 
Published: September 2024

AbstractDirectional control of far‐field thermal emission plays a key role in effective heat and energy transfer. However, conventional photonic strategies are challenging to concurrently control the polar and azimuthal angle of thermal emission over broadband. Here both polar and azimuthal angles of thermal emission are constrained to narrow ranges over broadband by introducing in‐plane anisotropy combined with magneto‐optical materials in the epsilon‐near‐zero (ENZ) wavelength range. The physical mechanism of tunable perfect absorption/emission is explored by investigating the evolution of multiple topological phase singularity pairs (TPSPs). The structure consisting of a magnetized gradient‐ENZ emitter and anisotropic spacer that exhibits high (>0.8) unidirectional emissivity (θ: 55°–79°, φ: 163.5°–196.5°) in the p‐polarization for a broad range of wavelength (22–26 µm) is demonstrated. The unveiled physics synergizing ENZ, anisotropy, and magneto‐optical properties that support broadband unidirectional thermal emission will bring new opportunities in applications such as thermal camouflaging, thermal photovoltaics, and infrared light sources.
Towards sustainable development: Institutions, perceptions, and innovation
Authors: Zhao Xiaoyang , Cui Dangdang , Ding Chante Jian 
Published: September 2024

AbstractThis study investigates the influence of the exogenous event of Chinese GIS (Government Inspection Supervision) on corporate environmental innovation. GIS significantly enhances green innovation in state‐owned enterprises (SOEs), according to difference‐in‐differences (DID) estimation. Moreover, executives' green perception significantly and positively moderates the constructive association between GIS and green innovation in SOEs. Additional analysis shows that inspection supervision encourages green innovation in SOEs by easing financial restrictions and boosting R&D spending. This effect is more prominent in areas where the intensity of government pollution source regulation is weaker and in enterprises with poorer performance in corporate social responsibility (CSR). Our research supports the conjecture that national governance can be a crucial determinant of green innovation, providing a valuable reference for accelerating China's green growth process and expanding the research perspective for the construction of governance systems in developing countries.
Deep learning methods for protein structure prediction
Authors: Qin Yiming , Chen Zihan , Peng Ye , Xiao Ying , Zhong Tian , Yu Xi 
Published: September 2024

AbstractProtein structure prediction (PSP) has been a prominent topic in bioinformatics and computational biology, aiming to predict protein function and structure from sequence data. The three‐dimensional conformation of proteins is pivotal for their intricate biological roles. With the advancement of computational capabilities and the adoption of deep learning (DL) technologies (especially Transformer network architectures), the PSP field has ushered in a brand‐new era of “neuralization.” Here, we focus on reviewing the evolution of PSP from traditional to modern deep learning‐based approaches and the characteristics of various structural prediction methods. This emphasizes the advantages of deep learning‐based hybrid prediction methods over traditional approaches. This study also provides a summary analysis of widely used bioinformatics databases and the latest structure prediction models. It discusses deep learning networks and algorithmic optimization for model training, validation, and evaluation. In addition, a summary discussion of the major advances in deep learning‐based protein structure prediction is presented. The update of AlphaFold 3 further extends the boundaries of prediction models, especially in protein‐small molecule structure prediction. This marks a key shift toward a holistic approach in biomolecular structure elucidation, aiming at solving almost all sequence‐to‐structure puzzles in various biological phenomena.
Effect of Solvent on the Optical Rotation of Azatryptophan Derivatives
Authors: Das Mitalee , Kulandai Felix , Kumar Hemantha , Kuppuswamy Prakasam , Subba Bandreddy , Hazra Sunit , Nimje Roshan , Gupta Anuradha , Bagadi Muralidhararao , Mathur Arvind , Roy Amrita , Duche Sharad 
Published: September 2024

ABSTRACTChirally pure enantiomers of differently protected 7‐azatryptophan derivatives (R‐3c, S‐3c, R‐3i, S‐3i, R‐3m, S‐3m, R‐3aa, and S‐3aa) were synthesized, which showed solvent‐dependent optical rotation. The obtained results not only exhibited changes in the values but also showed the variation in sign (− or +) with the different solvents studied. The change in optical rotation value was essentially attributed to the electron‐donating property, which can be correlated to the donor number of the solvents. There are two types of hydrogen bonds, intramolecular (i.e., form within the structure) and intermolecular (i.e., form with external groups such as solvents). These hydrogen bonds are responsible for the value and sign variations, and 1H NMR experiments were used to further characterize them. The NMR data suggested that hydrogen bond formation is occurring between the Fmoc NH group vicinal to the chiral center and donor group of the corresponding solvent.