bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel mechanism of inflammatory activation by Ebola virus matrix protein linked to the ebolavirus virulence
Authors: Yamaoka Satoko , M’Hamdi Zeineb , Wang Lin , Swenson Vaille A. , McNally Kristin L. , Lu Shao-Chia , Singh Reema , Saundh Stephanie L. , Zell Brady N. , Best Sonja M. , Barry Michael A. , Rasmussen Angela L. , Ebihara Hideki 
Published: October 2024

AbstractUncontrolled systemic inflammatory responses are a critical pathological feature of fatal Ebola virus (EBOV) infection. While some inflammatory responses may originate from mononuclear phagocytes (MNPs), non-immune cells vastly outnumber MNPs and may be an important source of inflammation. Here, we demonstrated that highly virulent EBOV induced a high and sustained pro-inflammatory response compared to less virulent ebolaviruses in non-MNPs through TLR4-independent NF-κB activation. We identified the EBOV matrix protein VP40 as a potent activator of NF-κB in non-MNPs, whose intrinsic inflammatory activation ability is higher than VP40 proteins from less virulent ebolaviruses. This suggests that VP40 is a novel virulence determinant inducing distinct degrees of pro-inflammatory responses among ebolaviruses. Mechanistically, VP40 activated the NF-κB signaling pathway, primarily via TNFR1 using a ligand-independent mechanism. These findings reveal mechanisms that may drive systemic inflammation and promote EBOV pathogenesis, suggesting potential therapeutic strategies to mitigate immune dysregulation in severe EBOV infections.
Loss of the Inv(16) OncogeneCBFB::MYH11Eliminates Leukemia from the Blood and Spleen, but not the Bone Marrow
Authors: Panda Sipra , Wang Yiqian , Becker Michelle , Dhir Arjun , Lam Calvin , Rivas Cecilia , Alemu Lemlem , Garrett Lisa , Swenson Samantha , Hyde R. Katherine 
Published: October 2024

AbstractInversion of chromosome 16 [inv(16)] is one of the most common chromosomal rearrangements in Acute Myeloid Leukemia (AML) and generates the fusion geneCBFB::MYH11 (CM), which initiates leukemogenesis. Patients with inv(16) at diagnosis invariably have the rearrangement at relapse, leading to the assumption thatCMis required after leukemic transformation. However, this has yet to be shown experimentally. Using a knock-in mouse that allows for deletion ofCMafter leukemia development, we found that loss of the fusion gene increased apoptosis and decreased colony growthin vitro. Interestingly, 5-20% of the colonies had successfully deletedCM. To test the role ofCM in vivo, we used an inducible shRNA knockdown (KD) construct against the fusion gene. We found that decreasedCMexpression eliminated leukemia cells from the peripheral blood and spleen, but not the bone marrow, despite these cells showing significant knockdown ofCMat the mRNA and protein levels. Furthermore, with prolonged KD ofCM, ∼40% of mice re-established disease while maintaining KD of the fusion gene. Our work indicates thatCMis required by leukemia cells in the spleen and blood, but that cells in the bone marrow can survive and re-establish disease independent of the fusion protein.
Tumor suppressor NME1/NM23-H1 modulates DNA binding of NF-κB RelA
Authors: Shahabi Shandy , Maurya Mano , Subramaniam Shankar , Ghosh Gourisankar 
Published: October 2024

ABSTRACTThe dimeric NF-κB family of transcription factors activates transcription by binding sequence-specifically to DNA response elements known as κB sites, located within the promoters and enhancers of their target genes. While most NF-κB remain inactive in the cytoplasm of unstimulated cells, a small amount of RelA, one of its members, persists in the nucleus, ensuring low-level expression of genes essential for homeostasis. Several cofactors have been identified that aid in DNA binding of RelA. In this study, we identify NME1 (nucleoside diphosphate kinase 1) as a cofactor that enhances RelA’s ability to bind κB sites within the promoters of a subset of its target genes, promoting their expression under both unstimulated and stimulated conditions. Depletion of NME1 influences activation or repression of several genes that are unresponsive to TNFα, despite containing κB sites in their promoters but not in clusters. This suggests that clustering of kB sites may be necessary for RelA-dependent transcription complex assembly. NME1 appears to act as a cofactor for other transcription factors to regulate these genes. NME1 does not directly contact κB DNA but interacts with RelA, with this interaction being further strengthened in the presence of κB DNA. Notably, NME1 alone has a marginal effect in enhancing RelA’s DNA binding, suggesting that NME1 likely cooperate with other cofactors to regulate DNA binding and transcription through RelA. These observations underscore the intricate assembly of transcription complexes centered on NF-κB.
Alzheimer’s disease-associated genotypes differentially influence chronic evoked seizure outcomes and antiseizure medicine activity in aged mice
Authors: Knox Kevin M. , Davidson Stephanie , Lehmann Leanne M. , Skinner Erica , Lo Alexandria , Jayadev Suman , Barker-Haliski Melissa 
Published: October 2024

Structured AbstractINTRODUCTIONAlzheimer’s disease (AD) patients are at greater risk of focal seizures than similarly aged adults; these seizures, left untreated, may worsen functional decline. Older people with epilepsy generally respond well to antiseizure medications (ASMs). However, whether specific ASMs can differentially control seizures in AD is unknown. The corneal kindled mouse model of acquired chronic secondarily generalized focal seizures allows for precisely timed drug administration studies to quantify the efficacy and tolerability of ASMs in an AD-associated genetic model. We hypothesized that mechanistically distinct ASMs would exert differential anticonvulsant activity and tolerability in aged AD mice (8-15 months) to define whether rational ASM selection may benefit specific AD genotypes.METHODSAged male and female PSEN2-N141I versus age-matched non-transgenic control (PSEN2 control) C57Bl/6J mice, and APPswe/PS1dE9versus transgene negative (APP control) littermates underwent corneal kindling to quantify latency to fully kindled criterion. Dose-related ASM efficacy was then compared in each AD model versus matched control over 1-2 months using ASMs commonly prescribed in older adults with epilepsy: valproic acid, levetiracetam, lamotrigine, phenobarbital, and gabapentin.RESULTSSex and AD genotype differentially impacted seizure susceptibility. Male PSEN2-N141I mice required more stimulations to attain kindling criterion (X2=5.521; p<0.05). Male APP/PS1 mice did not differ in kindling rate versus APP control mice, but they did have more severe seizures. There were significant ASM class-specific differences in acute seizure control and dose-related tolerability. APP/PS1 mice were more sensitive than APP controls to valproic acid, levetiracetam, and gabapentin. PSEN2-N141I mice were more sensitive than PSEN2 controls to valproic acid and lamotrigine.DISCUSSIONAD genotypes may differentially impact ASMs activity and tolerability in vivo with advanced biological age. These findings highlight the heterogeneity of seizure risk in AD and suggest that precisely selected ASMs may beneficially control seizures in AD, thus reducing functional decline.
Effect of Neoadjuvant Chemotherapy on Breast Conservative Surgery of Breast Cancer
Authors: Ahmed Mohamed Mostafa M , Abosenna Kamal Abdelrahman , Mohamed Basma Ahmed , Nagla Noha Mohamed 
Published: October 2024

AbstractBackgroundBreast conserving surgery (BCS) has been established as a safe and effective alternative way of total (modified radical) mastectomy for achieving loco regional control of female invasive breast cancer. There are still obstacles on generalizing neoadjuvant chemotherapy as a primary treatment of breast cancer even on early tumors, including large presenting tumors size relative to breast size especially on small breast sizes. Introducing preoperative (neoadjuvant) chemotherapy (NACT) has been argued to increase rates of neoadjuvant chemotherapy because of decreasing the overall tumor’s size.Aim of this studyThe aim of this work is to evaluate the efficacy of neoadjuvant chemotherapy on rates of breast conserving surgery on different molecular subgroups of breast cancer.Patients and methodsRecord of total of 44 patients with pathologically proven invasive breast cancer, who received neoadjuvant chemotherapy were included in this study. Eligibility of patients for breast conserving surgery before and after receiving neoadjuvant chemotherapy was measured in different molecular subtypes of breast cancer.ResultsNeoadjuvant chemotherapy increased eligibility for breast conserving surgery from 29% pre neoadjuvant chemotherapy to 77% post neoadjuvant chemotherapy. Highest rates of eligibility to neoadjuvant chemotherapy post neoadjuvant chemotherapy were achieved in HER-2 positive and Luminal A patients. Downstaging of the whole TNM disease stage decreased secondary to neoadjuvant chemotherapy in 89% of total study population. Luminal B patients showed the highest rates of downstaging. The average decrease in tumor size was 65.8% in response to neoadjuvant chemotherapy. HER-2 positive and triple negative patients showed the highest complete pathological response rates at 60.0% and 54.5% (p = 0.008).ConclusionNeoadjuvant chemotherapy increased the rate of breast conserving surgery with variation in response depending on the molecular subtype of the tumor. Her2+ tumors were the most sensitive subtypes to neoadjuvant chemotherapy with the highest breast conserving surgery eligibility following neoadjuvant chemotherapy.
Functional respiratory abnormalities in adults who vape daily
Authors: Lechasseur Ariane , Fortin Marc , Godbout Krystelle , Boulay Marie-Ève , Bergeron Keven , Routhier Joanie , Parent-Racine Geneviève , Roy Annie , Boutin Geneviève , Maltais François , Côté Andréanne , Morissette Mathieu C. 
Published: October 2024

AbstractDespite the widespread use of vaping, a very limited number of clinical studies have investigated the effects of this habit on the lungs of healthy individuals. Our group recently initiated theVapingAdverseLung and HeartEvents Cohort(VapALERT), a prospective study aiming to identify the impacts of vaping on respiratory and cardiovascular health. We elected to report early findings from the pulmonary function tests performed at the initial visit of the first 83 participants recruited so far. Almost 80% of volunteers with no diagnosis of lung disease and who vape daily have an abnormal airway reactivity to metacholine and/or lung clearance index and/or diffusion capacity. We can conclude from this study that adult individuals who vape daily are very likely to present asymptomatic functional respiratory abnormalities, especially airway hyperresponsiveness, ventilation heterogeneity and reduced gas diffusion regardless of past or current tobacco and/or cannabis smoking. Longitudinal studies are crucial to determine how respiratory abnormalities observed in individuals who vape will progress over time.
The Hunchback temporal transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in theDrosophilaNB5-2 lineage
Authors: Pollington Heather Q. , Doe Chris Q. 
Published: October 2024

AbstractInterneuron diversity within the central nervous system (CNS) is essential for proper circuit assembly. Functional interneurons must integrate multiple features, including combinatorial transcription factor (TF) expression, axon/dendrite morphology, and connectivity to properly specify interneuronal identity. Yet, how these different interneuron properties are coordinately regulated remains unclear. Here we used theDrosophilaneural progenitor, NB5-2, known to generate late-born interneurons in a proprioceptive circuit, to determine if the early-born temporal transcription factor (TTF), Hunchback (Hb), specifies early-born interneuron identity, includingmolecular profile,axon/dendrite morphology, andpresynapse targeting. We found that prolonged Hb expression in NB5-2 increases the number of neurons expressing early-born TFs (Nervy, Nkx6, and Dbx) at the expense of late-born TFs (Runt and Zfh2); thus, Hb is sufficient to promote interneuron molecular identity. Hb is also sufficient to transform late-born neuronal morphology to early-born neuronal morphology. Furthermore, prolonged Hb promotes the relocation of late-born neuronal presynapses to early-born neuronal presynapse neuropil locations, consistent with a change in interneuron connectivity. Finally, we found that prolonged Hb expression led to defects in proprioceptive behavior, consistent with a failure to properly specify late-born interneurons in the proprioceptive circuit. We conclude that the Hb TTF is sufficient to specify multiple aspects of early-born interneuron identity, as well as disrupt late-born proprioceptive neuron function.
Presynaptic filopodia form kinapses and modulate membrane mechanics for synchronous neurotransmission and seizure generation
Authors: Jain Akanksha , Kroll Jana , Webster Jack F. , Moss Jon , Ultanir Sila K. , Gonzalez-Sulser Alfredo , Rosenmund Christian , Cousin Michael A. , Ivanova Daniela 
Published: October 2024

AbstractThe structural stability of synapses directly contrasts with their functional plasticity. This conceptual dichotomy is explained by the assumption that all synaptic plasticity is generated via either electrical and/or biochemical signaling. Here, we challenge this dogma by revealing an activity-dependent presynaptic response that is physical in nature. We show that dynamic filopodia emerge during action potential discharge and transiently deform synaptic boutons to enhance connectivity. Filopodia generation requires neuronal activity, calcium and actin, and occurs in intact brain circuits and human brain. Mechanistically, their extension preserves synchronous neurotransmitter release by increasing presynaptic membrane tension. However, filopodia generation becomes maladaptive during dysregulated brain activity, exacerbating seizuresin vivo. Therefore, we provide direct evidence that presynaptic mechanical forces determine the extent and timing of synaptic signals.
Magnetic dipole contribution to the
γ
spectrum from
d−t
collisions
Authors: Timofeyuk N. K. 
Published: October 2024

One in about a few 105 sub-Coulomb d−t collisions is accompanied by the emission of a γ photon. The γ spectrum from these collisions is dominated by a 16.7 MeV peak, corresponding to the population of the p-wave 3/2− α−n ground-state resonance in the E1 transition from an intermediate d-wave α−n state, corresponding to the He5 excited state around 16.7 MeV. The strength of this spectrum at the large γ-energy endpoint decreases fast both due to kinematic factors and due to centrifugal repulsion between neutron and α particle at near-zero relative energies. However, no centrifugal repulsion would occur if s-wave α−n states were populated in a magnetic dipole transition. Therefore, one can expect that close to the γ-spectrum endpoint around 17.5 MeV the M1 transition could dominate, leading to additional counts in experimental spectra which could be easily misinterpreted as a background. This work presents calculations of the M1 contribution to the d+t→α+n+γ cross section caused both by convection and magnetization currents. While the first contribution was found to be negligible, the one from magnetization depends strongly on the choice of the model for the d−t channel scattering wave function. Using d−t interaction models from the literature, represented either by a gaussian of by a square well, resulted in unrealistically strong M1 contribution. Withoptical folding potential constructed in the present work, correctly representing the sizes of deuteron and triton, the M1 contribution near the endpoint is reduced to about 2% of the 16.7-MeV E1 peak. Arguments in favor of more detailed calculations, that could potentially change this number, are put forward.

Published by the American Physical Society
2024
CGC and saturation approach: Impact-parameter dependent model of perturbative QCD and combined HERA data
Authors: Roa Michael , Garrido José , Guevara Miguel 
Published: October 2024

In this paper we confront the color glass condensate and saturation approach of Contreras [Non-linear equation in the re-summed next-to-leading order of perturbative QCD: The leading twist approximation, ] with the experimental combined HERA data and obtain its parameters. The model includes two features that are in accordance with our theoretical knowledge of deep inelastic scattering. These consist of: (i) the use of analytical solution for the nonlinear Balitsky-Kovchegov evolution equation and () the exponential behavior of the saturation momentum on the impact parameter b-dependence, characterized by Qs∝exp(−mb) which reproduces the correct behavior of the scattering amplitude at large b in accord with Froissart theorem. The model results are then compared to data at small-x for the structure function of the proton F2, the longitudinal structure function FL, the charm structure function F2cc¯, the exclusive vector meson (J/ψ,ϕ,ρ) production and deeply virtual Compton scattering. We obtain a good agreement for the processes in a wide kinematic range of Q2 at small-x. Our results provide a strong guide for finding an approach, based on color glass condensate and saturation effective theory for high-energy QCD, to make reliable predictions from first principles and for forthcoming experiments like the Electron Ion Collider and the LHeC.

Published by the American Physical Society
2024
The configurational impact of e-commerce live streaming interactivity on consumer engagement behavior
Authors: Bolun Zhang , Yan Zhou , Minghui Jiang 
Published: October 2024

PurposeThis study aimed to verify the impact of e-commerce live streaming interactivity on consumer engagement behavior. Specifically, the multiple dimensions of interactivity and tie strength together form a high-level consumer engagement behavior.Design/methodology/approachData collection was carried out in the form of a questionnaire survey. Data from 416 respondents were analyzed by structural, followed by further test by fsQCA method.FindingsPerceived controllability, perceived responsiveness and perceived mutuality positively influence tie strength, and tie strength promotes consumer engagement behavior. Moreover, we found a configuration effect between interactivity and tie strength and revealed four configurations that can affect high-level engagement behavior.Research limitations/implicationsFirst, the SEM results show that the three dimensions of perceived interactivity positively impact tie strength: perceived controllability, perceived responsiveness, and perceived mutuality. And perceived personalization has no positive impact on tie strength. Second, the relevant results show that tie strength positively impacts consumer engagement behavior. Third, we find that the relationship between perceived interactivity and consumer engagement behavior may not be asymmetric, so the alternative combination of causal conditions may produce the same results. The fsQCA results revealed four configurations that can affect the level of consumer engagement behavior. And tie strength is the core condition.Practical implicationsThis study provides specific solutions for e-retailers and live streaming platforms to promote consumers' participation in engagement behavior in e-commerce live streaming.Originality/valueThis study transforms the linear impact of interactivity on consumer engagement behavior into a configuration effect for the first time, and enriches the live streaming commerce and consumer engagement behavior literature.
Socioeconomic position and sports in the US today: the economic and cultural capital of sports followers
Authors: Gemar Adam 
Published: October 2024

PurposeThis study aims to explore the relationship between social class and sports following in the United States, addressing a gap in research that has largely overlooked spectator sports in sociocultural analyses. The paper seeks to be an expanded update on previous work focusing on how socioeconomic factors such as income and education interact with sports engagement.Design/methodology/approachEmploying 2023 national survey data from the United States, this study analyzes the following of 31 different spectator sports in relation to income and education. The methods include the use of Cramer’s V statistic to assess associations between sports following and socioeconomic variables and multiple logistic regression analysis to understand the relative predictive power of these variables on sports following.FindingsThe study reveals significant socioeconomic associations in sports consumption patterns in American society, with higher income and education levels generally predicting an increased likelihood of following a wide range of sports. Notably, international soccer leagues like the English Premier League and La Liga showed the strongest associations with higher socioeconomic status, suggesting their following might serve as particularly potent markers of sociocultural distinction.Originality/valueThis research provides a contemporary empirical update to the study of social class and spectator sports in the US, expanding the understanding of the evolving landscape of sports consumption and its socioeconomic connections. It underscores the importance of considering sports following as a vital aspect of cultural engagement, reflecting and reproducing broader socioeconomic stratifications. The findings offer new insights into how factors of social class shape sports consumption today, highlighting the role of sports in the cultural life of different social classes in the US.
Influence of separation, collision and friction of rotating ring in sleeve on vibration characteristics of gas face seals
Authors: Teng Liming , Jiang Jinbo , Peng Xudong , Wu Fan , Zhao Wenjing 
Published: October 2024

Purpose
This study aims to understand how the assembly of rotating ring affects the axial forced vibration of gas face seals.

Design/methodology/approach
A three-mass kinematic model is established to investigate the axial movement of the rotating ring with bilateral constraints. The separation, collision and frictional sliding of the rotating ring in sleeve are discussed under rotor excitation. The effects of operating parameters and O-ring dynamic characteristics on the separation degree and film thickness disturbance are analyzed. A dimensionless axial characteristic force is defined to determine the critical conditions for the occurrence of separation. Several effective methods to eliminate the separation are proposed based on the adjustment of typical installation parameters.

Findings
Under rotor excitation, there may be two collisions between the rotating ring and the sleeve surfaces in one excitation period. This will cause self-excited vibration of the fluid film, increasing the risk of seal failure. The separation and collision can be prevented by increasing the equilibrium ratio, the installation radius of the O-ring on the outer surface of the rotating ring and the friction in the sleeve.

Originality/value
The results develop the modeling of multibody dynamics of gas face seals, enabling more accurate prediction of vibration characteristics.