bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meta‐analysis and meta‐regression of transcriptomic responses to water stress in Arabidopsis
Authors: Rest Joshua S. , Wilkins Olivia , Yuan Wei , Purugganan Michael D. , Gurevitch Jessica 
Published: January 2016

SummaryThe large amounts of transcriptome data available for Arabidopsis thaliana make a compelling case for the need to generalize results across studies and extract the most robust and meaningful information possible from them. The results of various studies seeking to identify water stress‐responsive genes only partially overlap. The aim of this work was to combine transcriptomic studies in a systematic way that identifies commonalities in response, taking into account variation among studies due to batch effects as well as sampling variation, while also identifying the effect of study‐specific variables, such as the method of applying water stress, and the part of the plant the mRNA was extracted from. We used meta‐analysis, the quantitative synthesis of independent research results, to summarize expression responses to water stress across studies, and meta‐regression to model the contribution of covariates that may affect gene expression. We found that some genes with small but consistent differential responses become evident only when results are synthesized across experiments, and are missed in individual studies. We also identified genes with expression responses that are attributable to use of different plant parts and alternative methods for inducing water stress. Our results indicate that meta‐analysis and meta‐regression provide a powerful approach for identifying a robust gene set that is less sensitive to idiosyncratic results and for quantifying study characteristics that result in contrasting gene expression responses across studies. Combining meta‐analysis with individual analyses may contribute to a richer understanding of the biology of water stress responses, and may prove valuable in other gene expression studies.
Identified metabolic signature for assessing red blood cell unit quality is associated with endothelial damage markers and clinical outcomes
Authors: Bordbar Aarash , Johansson Pär I. , Paglia Giuseppe , Harrison Scott J. , Wichuk Kristine , Magnusdottir Manuela , Valgeirsdottir Sóley , Gybel‐Brask Mikkel , Ostrowski Sisse R. , Palsson Sirus , Rolfsson Ottar , Sigurjónsson Olafur E. , Hansen Morten B. , Gudmundsson Sveinn , Palsson Bernhard O. 
Published: January 2016

BACKGROUNDThere has been interest in determining whether older red blood cell (RBC) units have negative clinical effects. Numerous observational studies have shown that older RBC units are an independent factor for patient mortality. However, recently published randomized clinical trials have shown no difference of clinical outcome for patients receiving old or fresh RBCs. An overlooked but essential issue in assessing RBC unit quality and ultimately designing the necessary clinical trials is a metric for what constitutes an old or fresh RBC unit.STUDY DESIGN AND METHODSTwenty RBC units were profiled using quantitative metabolomics over 42 days of storage in SAGM with 3‐ to 4‐day time intervals. Metabolic pathway usage during storage was assessed using systems biology methods. The detected time intervals of the metabolic states were compared to clinical outcomes.RESULTSUsing multivariate statistics, we identified a nonlinear decay process exhibiting three distinct metabolic states (Days 0‐10, 10‐17, and 17‐42). Hematologic variables traditionally measured in the transfusion setting (e.g., pH, hemolysis, RBC indices) did not distinguish these three states. Systemic changes in pathway usage occurred between the three states, with key pathways changing in both magnitude and direction. Finally, an association was found between the time periods of the metabolic states with the clinical outcomes of more than 280,000 patients in the country of Denmark transfused over the past 15 years and endothelial damage markers in healthy volunteers undergoing autologous transfusions.CONCLUSIONThe state of RBC metabolism may be a better indicator of cellular quality than traditional hematologic variables.
Nanofiltrated C1‐esterase‐inhibitor in the prophylactic treatment of bradykinin‐mediated angioedema
Authors: Greve Jens , Hahn Janina , Nordmann Melanie , Schuler Patrick J. , Bas Murat , Hoffmann Thomas K. , Hajdu Zuzana , Buchberger Maria , Strassen Ulrich 
Published: January 2016

BACKGROUNDPatients suffering from bradykinin‐induced angioedema show recurrent swelling of subcutaneous and submucosal structures. Increased bradykinin levels lead to an increase in vascular permeability and edema formation. Current therapy consists of B2 bradykinin receptor antagonists, C1‐esterase‐inhibitor (C1‐INH) concentrate, or the kallikrein inhibitor ecallantide. In most cases the treatment of acute attacks is sufficient. Prophylactic therapy is recommended only in severe cases. C1‐INHc has been shown a safe and efficient option. Its effect on the quality of life has not yet been analyzed.STUDY DESIGN AND METHODSPatients with inadequate disease control despite an “on‐demand therapy” including C1‐INHc and/or the B2 receptor antagonist icatibant were switched to long‐term prophylaxis consisting in an individual dose of intravenous C1‐INHc (Cinryze). None of the patients had been previously treated with ecallantide. Disease‐specific quality‐of‐life questionnaires and patient records were used for evaluation. Disease control, quality of life, adverse events, and administered dosage per month were compared for 6 months on on‐demand therapy and the following 6 months under prophylactic therapy.RESULTSData of seven patients with hereditary angioedema (HAE) and one patient with acquired angioedema were evaluated. Prophylactic therapy with Cinryze led to a significant and clinically relevant reduction in the overall attack frequency from 6.7 to 2.3 per month without relevant side effects. The frequency of severe attacks was reduced by 89% and quality of life significantly improved.CONCLUSIONProphylaxis with Cinryze led to a significantly improved quality of life in our cohort of patients with high‐frequency bradykinin‐induced angioedema attacks that were not sufficiently treated with on‐demand medication.
Implementing a blood management protocol during the entire perioperative period allows a reduction in transfusion rate in major orthopedic surgery: a before–after study
Authors: Rineau Emmanuel , Chaudet Aurélie , Chassier Claire , Bizot Pascal , Lasocki Sigismond 
Published: January 2016

BACKGROUNDPatient blood management (PBM) must be promoted in orthopedic surgery and relies on different strategies implemented during the entire perioperative period. Our aim was to assess whether the introduction of a pre‐, intra‐, and postoperative PBM protocol combining erythropoietin (EPO), ferric carboxymaltose (FCM), and tranexamic acid was effective in reducing perioperative transfusion and postoperative anemia.STUDY DESIGN AND METHODSIn a two‐phase prospective observational study, all patients admitted for total hip or knee arthroplasty were included the day before surgery. In Phase 1, use of EPO, iron, and tranexamic acid was left to the discretion of the anesthesiologists. In Phase 2, a protocol combining these treatments was implemented in the perioperative period. Perioperative hemoglobin levels and transfusion rates were recorded.RESULTSA total of 367 patients were included (184 and 183 in Phase 1 and 2, respectively). During Phase 2, implementing a PBM protocol allowed an increase in preoperative EPO prescription in targeted patients (i.e., with Hb < 13 g/dL; 18 [38%] vs. 34 [62%], p = 0.03) and in postoperative use of intravenous iron (12 [6%] vs. 32 [18%], p = 0.001) and tranexamic acid (157 [86%] vs. 171 [94%] patients, p = 0.02). In Phase 2, the number of patients who received transfusions (24 [13%] vs. 5 [3%], p = 0.0003) and of patients with a Hb level of less than 10 g/dL at discharge (46 [25%] vs. 26 [14%], p = 0.01) were reduced.CONCLUSIONIntroduction of a PBM protocol, using EPO, FCM, and tranexamic acid, reduces the number of perioperative transfusions and of patients with a Hb level of less than 10 g/dL at discharge.
ASVCP guidelines: quality assurance for portable blood glucose meter (glucometer) use in veterinary medicine
Authors: Gerber Karen L. , Freeman Kathleen P. 
Published: January 2016

AbstractPortable blood glucose meters (PBGM, glucometers) are a convenient, cost effective, and quick means to assess patient blood glucose concentration. The number of commercially available PBGM is constantly increasing, making it challenging to determine whether certain glucometers may have benefits over others for veterinary testing. The challenge in selection of an appropriate glucometer from a quality perspective is compounded by the variety of analytic methods used to quantify glucose concentrations and disparate statistical analysis in many published studies. These guidelines were developed as part of the ASVCP QALS committee response to establish recommendations to improve the quality of testing using point‐of‐care testing (POCT) handheld and benchtop devices in veterinary medicine. They are intended for clinical pathologists and laboratory professionals to provide them with background knowledge and specific recommendations for quality assurance (QA) and quality control (QC), and to serve as a resource to assist the provision of advice to veterinarians and technicians to improve the quality of results obtained when using PBGM. These guidelines are not intended to be all‐inclusive; rather they provide a minimum standard for management of PBGM in the veterinary setting.
Comparison of the gene encoding, and the predicted amino acid composition of, platelet membrane receptor subunit glycoprotein Ibα in members of the family Felidae
Authors: Boudreaux Mary K. , Christopherson Pete W. , Blair Cori 
Published: January 2016

BackgroundThere is minimal information regarding platelet receptors in the family Felidae. Comparative studies assist with identifying amino acids critical for protein structure and function.ObjectiveThe purpose of the study was to compare the gene encoding, and the predicted amino acid composition of, platelet membrane receptor subunit GPIbα in Felidae family members.MethodsGenomic DNA samples isolated from whole blood of 13 domestic cats and 50 big cats representing 8 different species were subjected to PCR using primers designed to flank the coding region of GPIbα in overlapping fashion. PCR products were separated via electrophoresis on agarose gels, and extracted products were submitted for sequencing. DNA sequences were used to predict the length and amino acid composition of the protein.ResultsVarying protein lengths were predicted in Felidae family members which were primarily due to polymorphisms in the variable number of tandem repeats region encoding the macroglycopeptide region of GPIbα. Other areas of the gene and predicted amino acid compositions were fairly conserved when compared to human sequences and between Felidae family members.ConclusionVarious polymorphisms within GPIbα, including length variants encoding the macroglycopeptide region, were identified in members of the family Felidae. More studies are needed to determine if a correlation exists between various polymorphisms and predisposition for hemorrhage or thrombosis as suggested in people.
Clinical and immunological responses of dust mite sensitive, atopic dogs to treatment with sublingual immunotherapy (SLIT)
Authors: DeBoer Douglas J. , Verbrugge Maria , Morris Mary 
Published: January 2016

BackgroundSublingual immunotherapy (SLIT) has been reported to be beneficial in people with atopic dermatitis (AD) and dust mite sensitivity. Evaluation of this therapy has not been reported in spontaneous canine AD.ObjectivesThe objective of this study was to preliminarily evaluate the effectiveness of an established SLIT protocol, as used in human patients, in dogs with AD.AnimalsTen dust mite sensitive dogs with spontaneous AD.MethodsDogs underwent a 6 month open trial of SLIT concurrently with decreasing dose oral methylprednisolone. Clinical evaluations and quantitative serum anti‐mite IgE and IgG levels were performed every 2 months.ResultsMean methylprednisolone use from the first 2 months of the study to the final 2 months declined from 10.2 to 4.3 mg/kg/2 months (P < 0.001, Student's paired t‐test); at 6 months, four dogs required no oral corticosteroid administration. Over the course of the study, median Canine Atopic Dermatitis Extent and Severity Index (CADESI)‐03 scores declined from 76.5 to 59; median pruritus scores declined from 65 to 37 (P < 0.02 and P < 0.01, respectively; Wilcoxon signed‐rank test). Pre‐ and post‐SLIT intradermal test scores for mite allergen were not significantly different over time. Median Dermatophagoides farinae (DF)‐specific IgE levels declined significantly from 150.2 × 103 AU/mL to 3.6 × 103 AU/mL (P < 0.05). Concurrently, median DF‐specific IgG levels increased from 18.5 × 106 AU/mL to 3923.4 × 106AU/mL (P < 0.05; Wilcoxon signed‐rank tests).Conclusions and clinical importanceSLIT treatment produced clinical improvement in dogs with dust mite‐associated AD and was associated with serological changes supporting this improvement. Further studies in larger numbers of dogs and those with polysensitization are warranted.
Severe anemia in cats with urethral obstruction: 17 cases (2002–2011)
Authors: Beer Kari Santoro , Drobatz Kenneth J. 
Published: January 2016

AbstractObjectiveTo characterize clinical parameters of cats with severe anemia due to suspected urinary bladder hemorrhage associated with urethral obstruction.DesignRetrospective case‐control study.SettingUniversity teaching hospital.AnimalsSeventeen cats with urethral obstruction and severe anemia (group “UO‐A”) that required transfusion were identified via medical record database search. Thirty cats with urethral obstruction and mild or no anemia (group “UO”) were included as controls.InterventionsNone.Measurements and Main ResultsThe median PCV of all cases at presentation was 28% (range, 9%–47%). Seven cats had PCV ≤20% at presentation, and all transfused cats had PCV ≤20% at the time of transfusion. Three cats did not receive a transfusion despite PCV ≤18%. Cats in the UO‐A group had a significantly longer duration of clinical signs (P = 0.001), and were more likely to have a history of previous urethral obstruction (P = 0.011), have a heart murmur (P = 0.002), have a gallop rhythm (P = 0.005), and have lower blood pressure (P = 0.007) compared to those in the UO group. Additionally, UO‐A cats had significantly lower pH, more negative base excess, higher BUN, and higher creatinine compared to UO cats. Duration of urinary catheterization was significantly (P = 0.016) longer in UO‐A cats. All UO cats survived to discharge, whereas 4/17 (23.5%) UO‐A cats were euthanized (P = 0.013).ConclusionsA history of previous urethral obstruction and longer duration of clinical signs may be important risk factors for severe anemia in UO cats. Additionally, UO‐A cats appeared to be more severely affected, as evidenced by lower blood pressure, more severe metabolic acidosis, higher BUN and creatinine, and worse outcome.
Persistent gross lipemia and suspected corneal lipidosis following intravenous lipid therapy in a cat with permethrin toxicosis
Authors: Seitz Marc A. , Burkitt‐Creedon Jamie M. 
Published: January 2016

AbstractObjectiveTo describe the observation of persistent gross lipemia and suspected corneal lipidosis following intravenous lipid therapy (IVLT) in a cat with permethrin toxicosis.Case SummaryA 5‐year‐old, spayed female, domestic short‐haired cat with permethrin toxicosis was treated with a high dose of IVLT as an adjunct treatment when it remained severely obtunded following traditional supportive care. The cat received intravenous 20% lipid emulsion as a 1.5 mL/kg bolus given over 10 minutes followed by a constant rate infusion of 0.25 mL/kg/min for 2 hours. The cat developed gross lipemia that persisted at least 48 hours after the single dose of IVLT. Changes consistent with corneal lipidosis were observed and resolved within 1 week after IVLT.New or Unique Information ProvidedThis is the first report documenting the complications of persistent gross lipemia and suspected corneal lipidosis in a cat following IVLT. This report underscores the off‐label, experimental nature of IVLT as a treatment for intoxication in cats.
Retrospective comparison of thromboelastography results to postmortem evidence of thrombosis in critically ill dogs: 39 cases (2005–2010)
Authors: Thawley Vincent J. , Sánchez Melissa D. , Drobatz Kenneth J. , King Lesley G. 
Published: January 2016

AbstractObjectiveTo determine whether there is an association between thromboelastography (TEG) data and necropsy evidence of thrombosis in a cohort of critically ill dogs.DesignRetrospective study (2005–2010).SettingUniversity teaching hospital.AnimalsThirty‐nine client‐owned critically ill dogs for which TEG was performed within 7 days of complete necropsy.InterventionsNone.Measurements and Main ResultsThrombi were found in 26 (67%) dogs. Spayed females (n = 20) were significantly more likely to have thrombosis (P = 0.0127). No significant association was found between presence of thrombosis and any TEG parameter, the calculated coagulation index, results of coagulation testing, type of vascular access, or clinical diagnosis. D‐dimers were significantly higher in dogs with thrombosis (P = 0.0207) and a weak positive correlation was found between D‐dimer value and number of sites of thrombosis (ρ = 0.18, P = 0.0045). Dogs with WBC > 16 × 103/μL were more likely to have thrombosis compared to others (odds ratio 5.56, 95% confidence interval 1.2–25.7, P = 0.025).ConclusionsThis study found no association between any TEG parameter and the presence of thrombosis on postmortem examination.
Re‐evaluating the residual risk of transfusion‐transmitted Ross River virus infection
Authors: Seed C. R. , Hoad V. C. , Faddy H. M. , Kiely P. , Keller A. J. , Pink J. 
Published: January 2016

Background and objectivesRoss River virus (RRV) is an enveloped, RNA alphavirus in the same antigenic group as chikungunya virus. Australia records an annual average of 5000 laboratory‐confirmed RRV infections. While RRV is currently geographically restricted to the Western Pacific, the capacity of arboviruses for rapid expansion is well established. The first case of RRV transfusion‐transmission was recently described prompting a comprehensive risk assessment.Materials and methodsTo estimate the RRV residual risk, we applied laboratory‐confirmed RRV notifications to two published models. This modelling generated point estimates for the risk of viraemia in the donor population, the risk of collecting a viraemic donation and the predicted number of infected components.ResultsThe EUFRAT model estimated the risk of infection in donors as one in 95 039 (one in 311 328 to one in 32 399) to one in 14 943 (one in 48 593 to one in 5094). The point estimate for collecting a RRV viraemic donation varied from one in 166 486 (one in 659 078 to one in 49 158) (annualized national risk) to one in 26 117 (one in 103 628 to one in 7729) (area of high transmission). The modelling predicted 8–11 RRV‐infected labile blood components issued in Australia during a 1‐year period.ConclusionConsidering the uncertainty in the modelled estimates, the unknown rate of RRV donor viraemia and the low severity of any recipient RRV infection, additional risk management for RRV in Australia will initially be restricted to strengthening the messaging to donors regarding prompt reporting of any postdonation illnesses.
An Alternative, Direct Approach for Arthroscopic Removal of Osteochondral Fragments in the Dorsal Pouch of the Proximal Intertarsal Joint of Horses
Authors: Compagnie Ellen , Drumm Niklas J. , de Heer Natascha , ter Braake Frerik 
Published: January 2016

ObjectiveTo describe an alternative technique and resulting outcomes for arthroscopic removal of osteochondral (OC) fragments from the proximal intertarsal joint (PIJ) using a direct approach without resection of the membrane between the tarsocrural joint (TCJ) and the PIJ.Study DesignRetrospective case series.AnimalsClient owned horses (n = 11) with Category 1 OC fragments in the PIJ.MethodsArthroscopic examination of the dorsal pouch of the TCJ was performed with horses positioned in dorsal recumbency using routine portals. A hook probe placed through one of the standard portals was used to lift the edge of the communication between TCJ and PIJ to help pass the arthroscope into the PIJ from the opposite standard portal. A third direct portal into the PIJ was created under arthroscopic visualization, and loose OC fragments (Category 1) were removed using the direct portal. Medical records, race records, and owner surveys were reviewed for case details and outcome.ResultsThe described technique allowed removal of all fragments in all 11 cases. No complications associated with the procedure were observed. Of the 9 horses with long term follow‐up available, 7 were used as intended (4/4 riding horses, 3/5 racehorses). Two horses did not enter an athletic career because of reasons unrelated to fragment removal.ConclusionA direct approach for arthroscopic removal of OC fragments of the PIJ using a third portal into this joint, without resection of the membrane between the TCJ and PIJ is a good alternative for removal of fragments at this site.
Long Term Outcome of Conservative Management or Surgical Treatment of Bovine Spastic Paresis: 79 Cases
Authors: De Vlamynck Caroline , Pille Frederik , Vlaminck Lieven 
Published: January 2016

ObjectiveTo report the long term clinical outcome of calves treated surgically or managed conservatively for bovine spastic paresis of the gastrocnemius (BSP‐G), quadriceps femoris muscle (BSP‐Q), or mixed muscle involvement (BSP‐M).Study DesignRetrospective case study.AnimalsCalves (n = 79) with bovine spastic paresis.MethodsMedical records of calves treated by partial tibial neurectomy or managed conservatively for bovine spastic paresis were analyzed for sex, breed, lineage history, and the onset, duration, and severity of clinical signs. Cases were classified as unilateral or bilateral BSP‐G, BSP‐Q, or BSP‐M. Long term follow‐up information was obtained by telephone questionnaire.ResultsThe study group included 26 BSP‐G (33%), 16 BSP‐Q (20%), and 37 BSP‐M (47%) calves. BSP‐M and BSP‐Q calves were significantly more bilaterally affected compared to BSP‐G calves. Twenty‐five of 26 BSP‐G calves were treated surgically; 86% had complete resolution of clinical signs. Twenty‐nine of 37 BSP‐M calves were treated surgically; 81.5% improved, but none completely recovered. In all of the conservatively managed BSP‐M calves, clinical signs gradually worsened. None of the BSP‐Q calves were treated surgically; in 66.7%, clinical signs gradually worsened and 33.3% of calves spontaneously improved.ConclusionPartial tibial neurectomy is advocated for the treatment of BSP‐G and in selected cases of BSP‐M. However, only partial resolution of clinical signs should be expected for BSP‐M. No surgical treatment exists for BSP‐Q calves, although spontaneous improvement is possible.