bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long bone histology of the subterranean rodent Bathyergus suillus (Bathyergidae): ontogenetic pattern of cortical bone thickening
Authors: Montoya‐Sanhueza Germán , Chinsamy Anusuya 
Published: September 2016

AbstractPatterns of bone development in mammals are best known from terrestrial and cursorial groups, but there is a considerable gap in our understanding of how specializations for life underground affect bone growth and development. Likewise, studies of bone microstructure in wild populations are still scarce, and they often include few individuals and tend to be focused on adults. For these reasons, the processes generating bone microstructural variation at intra‐ and interspecific levels are not fully understood. This study comprehensively examines the bone microstructure of an extant population of Cape dune molerats, Bathyergus suillus (Bathyergidae), the largest subterranean mammal endemic to the Western Cape of South Africa. The aim of this study is to investigate the postnatal bone growth of B. suillus using undecalcified histological sections (n = 197) of the femur, humerus, tibia‐fibula, ulna and radius, including males and females belonging to different ontogenetic and reproductive stages (n = 42). Qualitative histological features demonstrate a wide histodiversity with thickening of the cortex mainly resulting from endosteal and periosteal bone depositions, whilst there is scarce endosteal resorption and remodeling throughout ontogeny. This imbalanced bone modeling allows the tissues deposited during ontogeny to remain relatively intact, thus preserving an excellent record of growth. The distribution of the different bone tissues observed in the cortex depends on ontogenetic status, anatomical features (e.g. muscle attachment structures) and location on the bone (e.g. anterior or lateral). The type of bone microstructure and modeling is discussed in relation to digging behavior, reproduction and physiology of this species. This study is the first histological assessment describing the process of cortical thickening in long bones of a fossorial mammal.
Newcomer leader–member exchange: the contribution of anticipated organizational support
Authors: Zheng Dianhan , Wu Hao , Eisenberger Robert , Shore Lynn M. , Tetrick Lois E. , Buffardi Louis C. 
Published: September 2016

We surveyed 198 graduating college seniors at four points before and after they began a new job to examine the relationship between anticipated organizational support (AOS) and leader–member exchange (LMX) as mediated by information seeking. We found that AOS, assessed before the first day of work, was associated with subsequent LMX 3 months after work began. Furthermore, information seeking mediated the relationship between AOS and LMX. Moreover, LMX was associated with reduced psychological strain, lessened turnover intentions, and increased extra‐role behaviours 6 months following the start of work. These findings indicate that anticipation of future support before the onset of employment influences newcomers' LMX and its consequences.Practitioner points

Human resources practitioners and managers can prepare a better transition for newcomers by cultivating a supportive and caring view of the organization before the newcomers start their job. To enhance such anticipated organization support, the organization's positive valuation of the newcomers and concern with their welfare can be emphasized during job interviews with prospective employees and training sessions.

Educating supervisors to be responsive to newcomers' requests for information may contribute to their adjustment and help them meet organizational goals and objectives.
Photosynthetic activity and proteomic analysis highlights the utilization of atmospheric CO2 by Ulva prolifera (Chlorophyta) for rapid growth
Authors: Huan Li , Gu Wenhui , Gao Shan , Wang Guangce 
Published: September 2016

Free‐floating Ulva prolifera is one of the causative species of green tides. When green tides occur, massive mats of floating U. prolifera thalli accumulate rapidly in surface waters with daily growth rates as high as 56%. The upper thalli of the mats experience environmental changes such as the change in carbon source, high salinity, and desiccation. In this study, the photosynthetic performances of PSI and PSII in U. prolifera thalli exposed to different atmospheric carbon dioxide (CO2) levels were measured. Changes in photosynthesis within salinity treatments and dehydration under different CO2 concentrations were also analyzed. The results showed that PSII activity was enhanced as CO2 increased, suggesting that CO2 assimilation was enhanced and U. prolifera thalli can utilize CO2 in the atmosphere directly, even when under moderate stress. In addition, changes in the proteome of U. prolifera in response to salt stress were investigated. Stress‐tolerance proteins appeared to have an important role in the response to salinity stress, whereas the abundance of proteins related to metabolism showed no significant change under low salinity treatments. These findings may be one of the main reasons for the extremely high growth rate of free‐floating U. prolifera when green tides occur.
Ribavirin revisited in the era of direct‐acting antiviral therapy for hepatitis C virus infection
Authors: Feld Jordan J. , Jacobson Ira M. , Sulkowski Mark S. , Poordad Fred , Tatsch Fernando , Pawlotsky Jean‐Michel 
Published: September 2016

AbstractOver the past two decades, ribavirin has been an integral component of treatment for hepatitis C virus (HCV) infection, where it has been shown to improve the efficacy of (pegylated) interferon. However, because of treatment‐limiting side effects and its additive toxicity with interferon, the search for interferon‐ and ribavirin‐free regimens has been underway. The recent approvals of all‐oral direct acting antivirals (DAAs) have revolutionized the HCV therapeutic landscape, and initially it was expected that the role of ribavirin with DAA regimens would be eliminated. On the contrary, what we have witnessed is that ribavirin retains an important role in the optimal treatment of some subgroups of patients, particularly those that historically have been considered the most difficult to cure. Fortunately, it has also been recognized that the safety profile of ribavirin is improved when co‐administered with all‐oral DAA combinations in the absence of interferon. Despite the antiviral mechanism of action of ribavirin being poorly understood, we now have a range of novel insights into the potential role of ribavirin in all‐oral DAA HCV treatment and greater insight into the antiviral mechanism by which it continues to provide clinical benefit for defined patient groups.
Functional gastrointestinal disorders in Greek Children based on ROME III criteria: identifying the child at risk
Authors: Bouzios I. , Chouliaras G. , Chrousos G. P. , Roma E. , Gemou‐Engesaeth V. 
Published: September 2016

AbstractBackgroundFunctional gastrointestinal disorders (FGIDs) are a common, diverse group of disorders of unknown etiology, resulting in significant socieconomic burden. In this study, we aimed to assess the prevalence of FGIDs in children aged 6–18 years and examine their association with various demographic and socioeconomic parameters.MethodsThis was a school‐based, cross‐sectional study approved by the relevant government authorities. Informed consent was obtained by the legal representatives of all children who participated. Diagnoses of FGIDs were based on the Greek official translation of the ROME‐III questionnaire. Demographic and socioeconomic information were also collected.Key ResultsA total of 1588 children (51.8% females, mean age: 12.9±2.8 years) were included. The overall prevalence of any‐FGID was 23.1% (95% CI: 21.1–25.2). The most common FGIDs were functional constipation, n=231 (13.9%), abdominal migraine, n=84 (5.6%), aerophagia, n=58 (3.5%), and irritable bowel syndrome, n=48 (3.0%). Multiple logistic regression analysis on the probability of any‐FGID identified physical exercise, TV‐exposure, victimization, gender, parental educational level, number of children at home and number of adults at home as significant covariates for any‐FGID in the final model.Conclusions and InferencesFGIDs affect approximately 1 in 4 school‐aged children in Greece. The following characteristics are associated with a higher probability of any‐FGID: female gender, living in a non‐nuclear household, victimization, lower parental education level, infrequent physical activity, and high television exposure.
Mediating patienthood—from an ethics of to an ethics with technology
Authors: Kiran Asle H. 
Published: September 2016

AbstractThe changes that happen to healthcare services after the implementation of new assistive healthcare technologies (also called ‘welfare technology’ in the Nordic countries) concern more than increased efficiency and reducing healthcare expenditure. Of particular interest from an ethical point of view are the manners in which technologies shape the roles and identities of care receivers and healthcare personnel. The notion of ‘patienthood’ is explored in this paper as something that is both challenged by new technologies, and as something that is opened up for active and potentially positive reshaping when care receivers support their illness or frailty with assistive healthcare technologies. This dual effect of technologies (as both challenge and opportunity) requires a rethinking of ethics of technologies, which for most part have been preoccupied with ethical issues prior to the implementation of a new technology into a healthcare service. Ethics of technology should also contribute to the concrete efforts of care receivers to establish something approximating a ‘good patienthood’ in relation to a new technology, making it opportune to dub it, instead, an ethics with technology. This paper explores how assistive healthcare technologies impact on care receivers and the care situation, and in relation to the notion of patienthood, before turning to what this implies for an ethics that has as its goal to support care receivers to reach a life with the technology that is in line with their own notions of well‐being and a good life.
Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
Authors: Kudo Madoka , Kidokoro Satoshi , Yoshida Takuya , Mizoi Junya , Todaka Daisuke , Fernie Alisdair R. , Shinozaki Kazuo , Yamaguchi‐Shinozaki Kazuko 
Published: September 2016

SummaryAlthough a variety of transgenic plants that are tolerant to drought stress have been generated, many of these plants show growth retardation. To improve drought tolerance and plant growth, we applied a gene‐stacking approach using two transcription factor genes: DEHYDRATION‐RESPONSIVE ELEMENT‐BINDING 1A (DREB1A) and rice PHYTOCHROME‐INTERACTING FACTOR‐LIKE 1 (OsPIL1). The overexpression of DREB1A has been reported to improve drought stress tolerance in various crops, although it also causes a severe dwarf phenotype. OsPIL1 is a rice homologue of Arabidopsis PHYTOCHROME‐INTERACTING FACTOR 4 (PIF4), and it enhances cell elongation by activating cell wall‐related gene expression. We found that the OsPIL1 protein was more stable than PIF4 under light conditions in Arabidopsis protoplasts. Transactivation analyses revealed that DREB1A and OsPIL1 did not negatively affect each other's transcriptional activities. The transgenic plants overexpressing both OsPIL1 and DREB1A showed the improved drought stress tolerance similar to that of DREB1A overexpressors. Furthermore, double overexpressors showed the enhanced hypocotyl elongation and floral induction compared with the DREB1A overexpressors. Metabolome analyses indicated that compatible solutes, such as sugars and amino acids, accumulated in the double overexpressors, which was similar to the observations of the DREB1A overexpressors. Transcriptome analyses showed an increased expression of abiotic stress‐inducible DREB1A downstream genes and cell elongation‐related OsPIL1 downstream genes in the double overexpressors, which suggests that these two transcription factors function independently in the transgenic plants despite the trade‐offs required to balance plant growth and stress tolerance. Our study provides a basis for plant genetic engineering designed to overcome growth retardation in drought‐tolerant transgenic plants.
Dissociating parafoveal preview benefit and parafovea‐on‐fovea effects during reading: A combined eye tracking and EEG study
Authors: Niefind Florian , Dimigen Olaf 
Published: September 2016

AbstractDuring reading, the parafoveal processing of an upcoming word n+1 can influence word recognition in two ways: It can affect fixation behavior during the preceding fixation on word n (parafovea‐on‐fovea effect, POF), and it can facilitate subsequent foveal processing once word n+1 is fixated (preview benefit). While preview benefits are established, evidence for POF effects is mixed. Recently, it has been suggested that POF effects exist, but have a delayed impact on saccade planning and thus coincide with preview benefits measured on word n+1. We combined eye movement and EEG recordings to investigate and separate neural correlates of POF and preview benefit effects. Participants read lists of nouns either in a boundary paradigm or the RSVP‐with‐flankers paradigm, while we recorded fixation‐ or event‐related potentials (FRPs/ERPs), respectively. The validity and lexical frequency of the word shown as preview for the upcoming word n+1 were orthogonally manipulated. Analyses focused on the first fixation on word n+1. Preview validity (correct vs. incorrect preview) strongly modulated fixation times and electrophysiological N1 amplitudes, replicating previous findings. Importantly, gaze durations and FRPs measured on word n+1 were also affected by the frequency of the word shown as preview, with low‐frequency previews eliciting a sustained, N400‐like centroparietal negativity. Results support the idea that POF effects exist but affect word recognition with a delay. Lastly, once word n+1 was fixated, its frequency also modulated N1 amplitudes in ERPs and FRPs. Taken together, we separated immediate and delayed effects of parafoveal processing on brain correlates of word recognition.
Why Is Your Patient Still Short of Breath? Understanding the Complex Pathophysiology of Dyspnea in Chronic Kidney Disease
Authors: Salerno Fabio Rosario , Parraga Grace , McIntyre Christopher William 
Published: September 2016

AbstractDyspnea is one of the most common symptoms associated with CKD. It has a profound influence on the quality of life of CKD patients, and its underlying causes are often associated with a negative prognosis. However, its pathophysiology is poorly understood. While hemodialysis may address fluid overload, it often does not significantly improve breathlessness, suggesting multiple and co‐existing alternative issues exist. The aim of this article is to discuss the main pathophysiologic mechanisms and the most important putative etiologies underlying dyspnea in CKD patients. Congestive heart failure, unrecognized chronic lung disease, pulmonary hypertension, lung fibrosis, air microembolism, dialyzer bio‐incompatibility, anemia, sodium, and fluid overload are potential frequent causes of breathing disorders in this population. However, the relative contributions in any one given patient are poorly understood. Systemic inflammation is a common theme and contributes to the development of endothelial dysfunction, lung fibrosis, anemia, malnutrition, and muscle wasting. The introduction of novel multimodal imaging techniques, including pulmonary functional magnetic resonance imaging with inhaled contrast agents, could provide new insights into the pathophysiology of dyspnea in CKD patients and ultimately contribute to improving our clinical management of this symptom.
Short‐term treatment of equine wounds with orf virus IL‐10 and VEGF‐E dampens inflammation and promotes repair processes without accelerating closure
Authors: Bodaan Christa J. , Wise Lyn M. , Wakelin Kirsty A. , Stuart Gabriella S. , Real Nicola C. , Mercer Andrew A. , Riley Christopher B. , Theoret Christine 
Published: September 2016

AbstractHealing is delayed in limb wounds relative to body wounds of horses, partly because of sustained inflammation and inefficient angiogenesis. In laboratory animals, proteins derived from orf virus modulate these processes and enhance healing. We aimed to compare immune cell trafficking and the inflammatory, vascular, and epidermal responses in body and limb wounds of horses and then to investigate the impact of orf virus interleukin‐10 and vascular endothelial growth factor‐E on these processes. Standardized excisional wounds were created on the body and forelimb of horses and their progression monitored macroscopically until healed. Tissue samples were harvested to measure the expression of genes regulating inflammation and repair (quantitative polymerase chain reaction) and to observe epithelialization (histology), innate immune cell infiltration, and angiogenesis (immunofluorescence). Delayed healing of limb wounds was characterized by intensified and extended pro‐inflammatory signaling and exacerbated innate immune response, concomitant with the absence of anti‐inflammatory eIL‐10. Blood vessels were initially more permeable and then matured belatedly, concomitant with retarded production of angiogenic factors. Epithelial coverage was achieved belatedly in limb wounds. Viral proteins were administered to wounds of one body and one limb site/horse at days 1–3, while wounds at matching sites served as controls. Treatment dampened pro‐inflammatory gene expression and the innate immune response in all wounds. It also improved angiogenic gene expression, but primarily in body wounds, where it altered blood vessel density and myofibroblast persistence. Moreover, the viral proteins increased epithelialization of all wounds. The short‐term viral protein therapy did not, however, improve the healing rate of wounds in either location, likely due to suboptimal dosing. In conclusion, we have further detailed the processes contributing to protracted healing in limb wounds of horses and shown that short‐term administration of viral proteins exerts several promising though transient effects that, if optimized, may positively influence healing.
The Blueprint of a Minimal Cell: MiniBacillus
Authors: Reuß Daniel R. , Commichau Fabian M. , Gundlach Jan , Zhu Bingyao , Stülke Jörg 
Published: September 2016

SUMMARY

Bacillus subtilis
is one of the best-studied organisms. Due to the broad knowledge and annotation and the well-developed genetic system, this bacterium is an excellent starting point for genome minimization with the aim of constructing a minimal cell. We have analyzed the genome of
B. subtilis
and selected all genes that are required to allow life in complex medium at 37°C. This selection is based on the known information on essential genes and functions as well as on gene and protein expression data and gene conservation. The list presented here includes 523 and 119 genes coding for proteins and RNAs, respectively. These proteins and RNAs are required for the basic functions of life in information processing (replication and chromosome maintenance, transcription, translation, protein folding, and secretion), metabolism, cell division, and the integrity of the minimal cell. The completeness of the selected metabolic pathways, reactions, and enzymes was verified by the development of a model of metabolism of the minimal cell. A comparison of the
MiniBacillus
genome to the recently reported designed minimal genome of
Mycoplasma mycoides
JCVI-syn3.0 indicates excellent agreement in the information-processing pathways, whereas each species has a metabolism that reflects specific evolution and adaptation. The blueprint of
MiniBacillus
presented here serves as the starting point for a successive reduction of the
B. subtilis
genome.
Asian collisional subduction: A key process driving formation of the Tibetan Plateau
Authors: Replumaz Anne , Funiciello F. , Reitano R. , Faccenna C. , Balon M. 
Published: September 2016

Abstract
Using silicone slabs as a model analogue for lithospheric plates subducting into a box of glucose syrup, as an analogue of the mantle, we explore the subduction of continental lithosphere in a context of intercontinental collision. The continental indenter pushed by a piston, reproducing the collision, attached to a dense oceanic plate, subducts to two-thirds of the depth of the mantle box. We show that, surprisingly, the continental plate attached to the back wall of the box subducts, even if not attached to a dense oceanic slab. The engine of this subduction is not the weight of the slab, because the slab is lighter than the mantle, but the motion of the piston, which generates horizontal tectonic forces. These are transmitted to the back wall plate through the indenter and the upper plate at the surface, and by the advancing indenter slab through the mantle at shallow depth. We define this process as collisional subduction occurring in a compressional context. The collisional subduction absorbs between 14% and 20% of the convergence, and represents an unexplored component of collisional mass balance. The transmission of tectonic forces far from the collision front favors the formation of a wide plateau. Our experiments reproduce adequately the amount and geometry of the Asian lithosphere subduction episodes inferred during the collision, leading us to conclude that it reproduces adequately the physics of such process.
Dodging snowballs: Geochronology of the Gaskiers glaciation and the first appearance of the Ediacaran biota
Authors: Pu Judy P. , Bowring Samuel A. , Ramezani Jahandar , Myrow Paul , Raub Timothy D. , Landing Ed , Mills Andrea , Hodgin Eben , Macdonald Francis A. 
Published: September 2016

Abstract
The snowball Earth hypothesis predicts that low-latitude glaciation lasted millions of years while CO2 built up to critical levels to culminate in catastrophic deglaciation in a supergreenhouse climate. The Gaskiers Formation of eastern Newfoundland (Canada) has been attributed to a snowball glaciation event, but the lack of robust paleomagnetic data and precise geochronological constraints has precluded tests of the hypothesis. Here we present high-precision U-Pb zircon geochronology (chemical abrasion–isotope dilution–thermal ionization mass spectrometry) from eight tuffs from multiple distant stratigraphic sections that bracket glacial diamictites and the first appearance of large Ediacaran fossils. Including internal error, deposition of the Gaskiers diamictite on the Avalon Peninsula is constrained to have been between 580.90 ± 0.40 and 579.88 ± 0.44 Ma, and the Trinity diamictite on Bonavista Peninsula was deposited between 579.63 ± 0.15 and 579.24 ± 0.17 Ma. Assuming approximately synchronous deglaciation, these results imply a maximum duration for deposition of the Trinity diamictite of ≤340 k.y.; this is inconsistent with the multimillion year duration predicted by the snowball Earth hypothesis. Our geochronologic data also constrain the first appearance datum of Ediacaran fossils to &lt;9.5 m.y. after the Gaskiers glaciation. Thus, despite existing paleomagnetic constraints that indicate that marine ice sheets extended to low to middle latitudes, it appears that Earth narrowly escaped a third Neoproterozoic snowball glaciation just prior to the late Ediacaran expansion of metazoan ecosystems.