bluuebunny/crossref_metadata_2025_split huggingface dataset:
INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY ORMENIS ERIOLEPIS A MORROCAN ENDEMIC PLANT IN VARIOUS HUMAN CANCER CELL LINES
Authors: Belayachi Lamiae , Aceves-Luquero Clara , Merghoub Nawel , Mattos Silvia FernÃ¡ndez de , Amzazi SaaÃ¯d , Villalonga Priam , Bakri Youssef 
Published: March 2017

Background: Ormenis eriolepis Coss (Asteraceae) is an endemic Moroccan subspecies, traditionally named “Hellala”
or “Fergoga”. It’s usually used for its hypoglycemic effect as well as for the treatment of stomacal pain. As far as we
know, there is no scientific exploration of anti tumoral activity of Ormenis eriolepis extracts.
Materials and Methods: In this regard, we performed a screening of organic extracts and fractions in a panel of both
hematological and solid cancer cell lines, to evaluate the potential in vitro anti tumoral activity and to elucidate the
respective mechanisms that may be responsible for growth arrest and cell death induction. The plant was extracted
using organic solvents, and four different extracts were screened on Jurkat, Jeko-1, TK-6, LN229, SW620, U2OS, PC-3
and NIH3T3 cells.
Results: Cell viability assays revealed that, the IC50 values were (11,63±5,37μg/ml) for Jurkat, (13,33±1,67μg/ml) for
Jeko-1, (41,67±1,98μg/ml) for LN229 and (19,31±4,88μg/ml) for PC-3 cells upon treatment with Oe-DF and Oe-HE
respectively. Both the fraction and extract exhibited no effects on TK6 and NIH3T3. Cytometry analysis accompanied
by DNA damage signaling protein levels monitoring (p-H2A.X), showed that both the Dichloromethane Fraction and
Hexanic extract induce DNA double stranded breaks (DSBs) accompanied by cell cycle arrest in G1 (Jurkat, Jeko -1
and LN22) and G2/M (PC-3) phases which is agreed with the caspase activity observed. Additional experiments with
selective inhibitors of stress and survival pathways (JNK, MAPK, Rho, p53, and JAK3) indicated that none of these
pathways was significantly involved in apoptosis induction. The bioactive compound analysis by CG/MS indicated that
the major compounds in Oe-DF were: Linoleic Acid (15,89%), Podophyllotoxin (17,89%) and Quercetin (22,95%). For
Oe-HE the major molecules were: Linoleic Acid (9,76%), α-curcumene (7,07%), α-bisabolol (5,49%), Campesterol
(4,41%), Stigmasterol (14,08%) and β-sitosterol (7,49%).
Conclusion: Our data suggest that bioactive compounds present in Ormenis eriolepis show significant anti proliferative
activity inducing cell cycle arrest and cell death operating through apoptosis pathway.
THE BIOLOGICAL ACTIVITIES OF MOLTKIA AUREA BOISS., AN ENDEMIC SPECIES TO TURKEY
Authors: Balpinar Neslihan , Okmen Gulten 
Published: March 2017

Background: Staphylococcus aureus is the direct reason of mastitis. Mastitis is a disease characterized by pathological
changes in mammary glands as well as physical, chemical, bacteriological changes in milk. This disease causes loses in
milk yield and quality. In recent years, it is reported that mastitis pathogens have developed a resistance to antibiotics
as a natural consequence of widespread use of it. Today’s researches are focused on discovering and using new
antibiotics against bacteria. The aim of this paper is to examine the antibacterial properties of Moltkia aurea Boiss.][o]
(an endemic species to Turkey), and its other biological activities.
Materials and Methods: All of the extracts were tested by disc diffusion assay in order to screen antibacterial activity.
MIC values were evaluated as antibacterial activities of plant extracts. The non-enzymatic antioxidative activities
including DPPH radical scavenging effects were studied in vitro.
Results and Conclusions: Results shown that the extracts had strong antibacterial effects on three bacteria (S. aureus-
17, S. aureus-18 and CNS-37) and the range of inhibition zone was 4-6mm. This three bacteria screened the lowest
sensitivity to 65000 μg /mL concentration. Besides, the extracts were tested for non-enzymatic antioxidant activities.
As a result, the methanol extract of the flower displayed a strong antioxidant activity. The various extracts of Moltkia
aurea have different antibacterial and antioxidant properties.
ANTITUMOR AND APOPTOTIC EFFECTS OF CUCURBITACIN A IN A-549 LUNG CARCINOMA CELLS IS MEDIATED VIA G2/M CELL CYCLE ARREST AND M-TOR/PI3K/AKT SIGNALLING PATHWAY.
Authors: Wang Wen-dong , Liu Yan , Su Yuan , Xiong Xian-zhi , Shang Dan , Xu Juan-juan , Liu Hong-ju 
Published: March 2017

Background: The main aim of this study was to demonstrate the antitumor potential of cucurbitacin A on A-549 NSCLC
(non-small cell lung cancer cells). The effects of Cucurbitacin A on apoptotic induction, cell physic, cell cycle failure and
m-TOR/PI3K/Akt signalling pathway were also investigated in the present study.
Materials and Methods: MTT assay and clonogenic assay were carried out to study effects of this compound on cell
cytotoxicity and colony forming tendency in A-549 cells. Moreover, phase and fluorescence microscopic techniques were
used to examine the effects on cell morphology and induction of apoptosis. The effects on cell cycle phase distribution were
investigated by flow cytometry and effects on m-TOR/PI3K/Akt signalling proteins were assessed by western blot analysis.
Results: Results showed that cucurbitacin A induced dose-dependent cytotoxic effects along with suppressing the colony
forming tendency in these cells. Cucurbitacin A also induced morphological changes in these cells featuring chromatin
condensation, cell shrinkage and apoptotic body formation. G2/M phase cell cycle collapse was also induced by
Cucurbitacin A along with inhibition of expression levels of m-TOR/PI3K/Akt proteins.
Conclusions: In conclusion, cucurbitacin A inhibits cancer growth in A-549 NSCLC cells by inducing apoptosis, targeting
m-TOR/PI3K/Akt signalling pathway and G2/M cell cycle.
BIOACTIVE COMPONENTS OF GYNURA DIVARICATA AND ITS POTENTIAL USE IN HEALTH, FOOD AND MEDICINE:A MINI-REVIEW
Authors: Xu Bing-Qing , Zhang Yu-Qing 
Published: March 2017

Background: G. divaricata (L.) DC belongs to genus the Gynura Cass which is a kind of perennial herb that has good
health protection efficacy and is especially used widely in medicine and functional food. It is one of the most famous
traditional Chinese medicinal herbs and is usually used to cure bronchitis, pulmonary tuberculosis, kink cough,
rheumatism, diabetes, and so on. It has a long history for the treatment of diabetes mellitus in the folk medicine. This
review is aimed at gathering all information relating to G. divaricata and obtaining new insights for further studies on G.
divaricata
Materials and Methods: PubMed, Springer Link, Web of Science, Science Direct and Research Gate were used for the
literature search. The key search terms included G. divaricata isolation and identification, flavonoids and their
combinations without language restriction. The period for the search is from year 1979 to 2016.
Results: The main chemical components were listed, and the folk application, the extraction and separation methods of
main chemical components, pharmacological effects of G. divaricata were discussed, which further demonstrated the
plant’s value as health food and medicine.
Conclusion: The present review is of great significance to the development of new medicinal resources and health food
of G. divaricata
PURIFICATION AND FRACTIONAL ANALYSIS OF METHANOLIC EXTRACT OF WEDELIA TRILOBATA POSSESSING APOPTOTIC AND ANTI-LEUKEMIC ACTIVITY
Authors: Venkatesh Uday , Javarasetty Chethan , Murar Satish Kumar 
Published: March 2017

Background: Wedelia trilobata (L.) Hitch (WT), commonly known as yellow dots or creeping daisy, is a shrub
possessing potent biological activities, and is traditionally used a medicinal plant in Ayurveda, Siddha and Unani
systems of medicines, and it has also been tried against leukemia cell line MEG- 01. In the present study, purification
and screening of the plant was done for bioactive compounds in methanolic extract of WT for apoptotic and antileukemia
activity.
Materials and methods: The methanolic extract of WT was initially purified through thin layer chromatography
(TLC) and screened for the apoptotic and anti-leukemia activities. The positive band of TLC was subjected to silica gel
column chromatography for further purification and the fractions obtained from it were screened again for antileukemia
activity through thymidine uptake assay and apoptotic activity by DNA fragmentation, nuclear staining and
flow cytometry assays. The fraction with positive result was subjected to HPLC for analysis of bioactive components.
Results: Out of many combinations of solvents, the methanol and dichloromethane combination in the ratio 6:4 has
revealed two bands in TLC, among which the second band showed positive results for apoptotic and anti-leukemic
activities. Further purification of second band through silica gel chromatography gave five fractions in which the 3rd
fraction gave positive results and it shows single peak during compositional analysis through HPLC.
Conclusion: The single peak revealed through HPLC indicates the presence of pure compound with apoptotic and antileukemia
activities encouraging for further structural analysis.
ANTI-VIRAL EFFECT OF HERBAL MEDICINE KOREAN TRADITIONAL CYNANCHUM PANICULATUM (BGE.) KITAG EXTRACTS
Authors: Kim Woong , Oh Tae-Seok , Park Youn-Jin 
Published: March 2017

Background: Pestiviruses in general, and Bovine Viral Diarrhea (BVD) in particular, present several potential targets
for directed antiviral therapy.
Material and Methods: The antiviral effect of Cynanchum paniculatum (Bge.) Kitag (Dog strangling vine: DS)
extract on the bovine viral diarrhea (BVD) virus was tested. First, a cytotoxicity test in MDBK (Madin-Darby bovine
kidney) cells was done with all organic extract concentrations.
Results: The cytotoxic concentration CC50 for the ethyl acetate (EA) extracts was 18.2 ug/ml. In the tissue culture,
infectious dose (TCID50) assay, the BVD virus decreased when treated with 18.2 ug/ml of the ethyl acetate extracts.
Conclusion: Ethyl acetate extracts and fractions of the DS extract could be used as a potential antiviral for BVD.
INVESTIGATION ON CHEMICAL COMPOSITION, ANTIMICROBIAL, ANTIOXIDANT, AND CYTOTOXIC PROPERTIES OF ESSENTIAL OIL FROM DRACOCEPHALUM KOTSCHYI BOISS
Authors: Ashrafi Behnam , Ramak Parvin , Ezatpour Behrouz , Talei Gholam Reza 
Published: March 2017

Background: Dracocephalum kotschyi Boiss is a herb with wide-spread applications. Lorestan traditional healers have
applied it for the treatment of rheumatoid diseases and stomach disorders.
Materials and methods: Hydrodistillation process was used for essential oil extraction, the extracted essential oil was
then analyzed through combination of capillary GC–FID, GC–MS and RI. The in vitro antimicrobial, antioxidant and
cytotoxic activities of this essential oil were examined. Results indicate that the essential oil has a broad range of antimicrobial
activity against all of the tested microorganisms.
Results: The 50% of cytotoxic concentrations was 26.4 μg/ml and 4266.7 μg/ml for Hela cells and human
lymphocytes, respectively. The oil cytotoxicity against the human tumor cell line was far higher than the amount
required for human healthy cells. Conversely, the essential oil’s IC50 value of 49.2 μg/ml in the DPPH assay, could be
regarded as its strong antioxidant potential.
Conclusion: According to the data obtained, it can be concluded that D. kotschyi essential oil could be applied as a safe
antibacterial and antioxidant agent for food and pharmaceutical purposes.
SURVEY OF DENTAL STUDENTS’ ATTITUDE REGARDING ORIENTAL MEDICINE/COMPLEMENTARY AND ALTERNATIVE MEDICINE: COMPARISON BETWEEN TWO JAPANESE DENTAL SCHOOLS
Authors: Kameyama Atsushi , Toda Kazuo 
Published: March 2017

Background: The present study aimed to examine the impact of “curricula for undergraduate education in oriental
medicine (OM)/complementary and alternative medicine (CAM)” on student awareness of OM. A questionnaire survey
was conducted involving the Nagasaki University School of Dentistry (NUSD), a university that implements education
in OM as part of its undergraduate curriculum, and Tokyo Dental College (TDC), which does not teach OM.
Materials and methods: The third- and fifth-year students of both NUSD and TDC underwent the anonymous
questionnaire survey, which included questions regarding their knowledge of OM and CAM, interests in these subjects,
and their opinions on the necessity of teaching OM in the undergraduate dental education, and the results were
collected for analysis.
Results: Whereas 33% of 5th year NUSD students had knowledge of OM/CAM was 33%, only 10% of 5th year TDC
students reported knowledge on the subject. 69% of 5th year NUSD students interested in OM/CAM, while 5th year
TDC students who interest them were only 45%. Although 77% of 5th year NUSD students were in favor of OM
education implemented in the Faculty of Dentistry, the percentages of TDC students of that were smaller (46% in 3rd
year and 48% in 5th year). Whereas 26% of 5th year TDC students did not recognize the necessity of oriental medicine
education, only one 5th year NUSD student (2%) did not so.
Conclusion: Introduction of education in OM in the undergraduate dental education program helps students to increase
their interests in dental clinical applications.
IN VITRO EFFECTS OF A COMMERCIAL HERBAL MEDICINE USED AS AFRICAN TRADITIONAL MEDICINE ON HUMAN NEUTROPHILS
Authors: Mothibe Mmamosheledi Elsie , Kahler-Venter Christinah , Osuch Elzbieta 
Published: March 2017

Background: Commercial herbal medicines (CHMs) being marketed as immune boosters or tonics, have gained
widespread popularity. The many herbal mixtures sold have not been tested for efficacy and safety, despite their
modern packaging and presentations. It is imperative that these herbal mixtures be investigated for their effects on
human neutrophils.
Methods: The selected herbal mixture (HM), Stametta™ Body healing liquid, is common in retail outlets in Pretoria,
South Africa (SA) and is used as an immune booster or intended to strengthen the body. Isolated neutrophils as well as
those in whole blood phagocytes were obtained from blood samples collected from consenting healthy adult volunteers.
The neutrophils were incubated with the HM at different strengths, and taken through a luminol-enhanced
luminescence assay, using activators- phorbol myristate acetate and N-formyl-methionyl-leucyl-phenylalanine.
Results: The HM had variable stimulatory and inhibitory effects on the luminescence activity of healthy isolated and
non-isolated human neutrophils. The effects, ranging from weak to potent were either directly or inversely related to
the concentration of the HM and were mediated through a direct protein kinase C activating mechanism and an indirect
formyl peptide receptor-linked mechanism.
Conclusion: The findings have shown the immunomodulatory potential of Stametta TM. The in vitro inhibitory and
stimulatory effects on neutrophils which are furthermore time-based, suggest variable effects on the immune system,
which may be beneficial as well as risky. The effects at different concentrations highlight the importance of appropriate
dosing. It would therefore be prudent to caution users of this commercial herbal medicine accordingly.
Para onde nos leva a principal política de mobilidade urbana na Região Metropolitana do Recife? Do Modelo Tradicional às Novas percepções sobre desenvolvimento
Authors: Béhar Alexandre Hochmann , Dourado Débora Paschoal 
Published: March 2017

<p>Diversas são as críticas direcionadas ao modelo tradicional de desenvolvimento, marcado pelo viés econômico. Neste sentido, o Estado apresenta-se como principal agente, a cidade o palco desta crise, e a mobilidade urbana uma de suas significativas representações. Assim, este estudo se propõe a analisar as perspectivas de desenvolvimento subjacentes a uma política pública de mobilidade urbana na Região Metropolitana do Recife. Foi realizada uma pesquisa qualitativa básica, consolidada por meio da análise de conteúdo, cujos resultados apresentados indicam que, apesar do discurso dos governantes, tais políticas públicas ainda encontram-se direcionadas às antigas práticas desenvolvimentistas.</p>
Peningkatan keterampilan proses sains melalui percobaan sederhana anak usia 5-6 tahun di TK-IT Albina Ternate
Authors: Wahid Santi M. J. , Suyanto Slamet 
Published: March 2017

Penelitian ini bertujuan untuk mengetahui peningkatan keterampilan proses sains melalui percobaan sederhana pada anak usia 5-6 tahun di TK IT Albina Kota Ternate. Jenis penelitian ini adalah penelitian tindakan kelas (kolaboratif) yang terdiri dari tiga siklus & enam pertemuan. Subjek penelitian adalah anak-anak kelas B2 yang berjumlah 19 anak terdiri dari 8 anak laki-laki dan 11 anak perempuan. Teknik pengumpulan data yaitu observasi dan catatan lapangan. Instrumen menggunakan lembar observasi Keterampilan Proses Sains (KPS) anak dan lembar observasi kegiatan guru selama kegiatan pembelajaran. Data yang terkumpul dianalisis secara deskriptif kualitatif dan kuantitatif. Hasil penelitian ini menunjukkan bahwa peningkatan keterampilan proses sains anak secara keseluruhan pada siklus I sebesar 59,38%, siklus II sebesar 66,12% dan siklus III sebesar 78,82% dengan keterampilan proses sains masing-masing indikator KPS selama tiga siklus sebagai berikut: (1) KPS Membuat dugaan adalah 53,84%, 60,53%, 73,55%, (2) KPS mengamati adalah 65,02%, 71,71%, 85%, (3) KPS mengklasifikasi adalah 64,25%, 69,08%, 84,01%, dan (4) KPS mengkomunikasikan sebesar 54,39%, 63,08%, 72,70%. Aktivitas kegiatan guru mengalami peningkatan sebesar 36,84% dan 47,37% siklus I, 63,16% dan 73,68% siklus II, 89,47% dan 94,74% siklus III.Kata kunci: keterampilan proses sains, percobaan sederhana, anak usia 5-6 tahun Improving KPS Through the Simple Experiment for the 5-6 Year-Old Students in TK-IT Albina Ternate AbstractThis research is aimed to find out the improvement of the science skill through the simple experiment for the 5-6 year-old students in TK IT Albina, Ternate. This research was a Classroom Action Research (collaborative) consisted of three cycles with six meetings. The subjects of the research were the students of Class B2 in TK IT Albina Ternate which were 19 students consisting of 8 boys and 11 girls. The data collection techniques were by observing and making field notes. The instruments of the research used the students Science Process Skills (SPS) observation sheets and teacher observation sheets during the process of studying. The data then were analyzed by using descriptive qualitative and quantitative methods. The results of this research indicats the improvement of science process skill entirely in the Cycle I is 59.38 %, in Cycle II is 66.12%, and in Cycle III is 78.82% with each SPS indicator as (1) SPS made judgment is 53.84%, 60.53%, 73.55%, (2) SPS observing is 65.02%, 71.71%, and 85%, (3) SPS classifiying is 64.25%, 69.08%, 84.01%, and (4) SPS communicating is 54.39%, 63.08%, 72.70%. In Cycle I The teacher’s activities improve from 36.84% to 47.37%, in Cycle II much as 63,16% and 73.68%, and in cycle III is 89.47%.Keywords: science process skill, simple experiment, 5-6 years-old students
Potensi gugus PAUD untuk pembinaan paska pelatihan pendidik anak usia dini dengan menggunakan lesson study
Authors: Fauzi Ikka Kartika Abbas 
Published: March 2017

Penelitian ini bertujuan untuk mencari peluang bagi peningkatan kompetensi pendidik PAUD paska pelatihan dengan menggunakan lesson study melalui wadah pembinaan yang sudah ada, yaitu Gugus PAUD. Alasannya hanya 20,4 % tenaga pendidik alumni Diklat yang langsung mampu menerapkan hasil pelatihannya. Di sisi lain, banyak Gugus PAUD yang belum optimal dimanfaatkan sebagai wadah pembinaan pendidik PAUD. Penelitian ini menggunakan metoda kualitatif dengan teknik pengumpulan data wawancara, observasi dan dokumentasi. Data dianalisis dengan menggunakan flow model . Hasil penelitian menunjukkan bahwa Gugus PAUD memiliki peluang untuk mengembangkan pembinaan Paska Pelatihan dengan menggunakan model pembinaan Lesson Study karena memiliki potensi yang relevan dengan penyelenggaraan lesson study, yaitu : terstruktur dan terorganisir, pembinaan terus menerus dari pihak yang berwenang, kebersamaan, pendekatan partisipatif, kemampuan bermitra dan penumbuhan komunitas belajar. Dalam pelaksanaannya perlu memperhatikan factor pendorong dan penghambat pembinaan di Gugus PAUD.Kata kunci : potensi gugus PAUD, lesson study , pembinaan pasca pelatihan Potential Gugus PAUD for Coaching Post Training Early Childhood Educators by Using Lesson Study AbstractThis study aims to explore opportunities for increased competence post training early childhood educators with using lesson study through the existing institution , namely Gugus PAUD. The reason only 20.4% of educators alumnus of training that is directly able to apply their training. On the other hand, many Gugus PAUD that has not been optimally utilized as a forum for coaching early childhood educators. This study uses qualitative methods with data collection techniques of interview, observation and documentation. Data were analyzed using flow model . The results showed that the Gugus PAUD has the opportunity to develop coaching after the training using coaching model Lesson Study as it has potential relevance for the implementation of lesson study, namely: structured and organized, coaching continuously from the authorities, togetherness, participatory approach, the ability to partner and growth of a learning community. In the execution should also pay attention to supporting factors and inhibiting factors in Gugus PAUD development.Keywords: potential Gugus PAUD, lesson study, post-training coaching
Desain model wisata belajar di Kebun Binatang Gembiraloka Yogyakarta sebagai laboratorium luar kampus
Authors: Sujarwo Sujarwo , Samsi Ibnu , Wibawa Lutfi 
Published: March 2017

Penelitian ini bertujuan untuk mendekripsikan desain model wisata belajar di Kebun Binatang Gembira Loka Yogyakarta. Penelitian tahap awal ini tersusun desain model wisata belajar di GL Zoo merujuk pada penelitian tindakan. Dari informasi yang diperoleh pada tahap pengumpulan data, selanjutnya peneliti mendesain produk yang berupa desain model wisata belajar, menyusun panduan kegiatan, panduan pendampingan, pelaksanaan dan refleksi. Untuk mendukung keberhasilan wisata belajar perlu adanya panduan yang dapat dijadikan acuan dalam kegiatan pembelajaran. Adapun komponen dalam desain model ini terdiri dari; (1) calon peserta, (2) memilih peserta, (3) Bentuk Kegiatan Wisata Belajar (penyambutan, bina suasana, tour to zoo, zona alam, pojok kreatif, permainan, dan refleksi), (4) Materi Kegiatan sesuai jenis kegiatannya, (5) media, (6) bahan Ajar, (7) penilaian, (8) sosialisasi program, (9) pelaksanaan program, (10) evaluasi program dan (11) pendampingan, Model ini dilengkapi dengan panduan kegiatan, panduan pembelajaran, dan panduan materi wisata belajarKata Kunci: desain model, wisata belajar, laboratorium luar kampus Design of Study-Tour Model in Gembira Loka Zoo Yogyakarta as an Outdoor Laboratory AbstractThe paper is aimed at describing the design of study-tour model in Gembira Loka Zoo Yogyakarta. This initial-phase research comprised the design of study-tour model in Gembira Loka Zoo which refered to action reseach. On the basis of the information gained from the data collection, the researchers designed the product in the form of study-tour model, arranged the guides of activities, accompaniment, implementation, and reflection. In order to support the success of study-tour, a guide which could be used as a reference in the learning activity is needed. The components of the model design consisted of (1) candidates of participant, (2) participants selection, (3) study-tour activity (welcoming, atmosphere building, zoo tour, nature zone, creativity corner, games, and reflections), (4) activity materials suitable to the types of the activity, (5) media, (6) learning material, (7) assessment, (8) program socialization, (9) program implementation, (10) program evaluation, and (11) accompaniment. This model was completed with guides on the activity, learning, and study-tour materials.Keywords: design model, tourism learning laboratory campus affairs