bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effective tricuspid regurgitation reduction is associated with renal improvement and reduced heart failure hospitalization
Authors: Felbel Dominik , von Winkler Juliana , Paukovitsch Michael , Gröger Matthias , Walther Elene , Andreß Stefanie , Schneider Leonhard , Markovic Sinisa , Rottbauer Wolfgang , Keßler Mirjam 
Published: October 2024

BackgroundSeveral studies have demonstrated an association between tricuspid regurgitation (TR) and organ dysfunction including hepatic and renal insufficiency. Improvement of liver function following transcatheter edge-to-edge repair (T-TEER) has already been linked to reduction of venous congestion due to TR reduction. This study analyzes whether TR-reduction using T-TEER is also associated with improved renal function.Methods and resultsThe TRIC-ULM registry includes 92 selected patients undergoing T-TEER between March 2017 and May 2023. Estimated glomerular filtration rate (eGFR) improvement was evident in 53 patients (57%) at 3-months follow-up (FU) and defined by FU eGFR &gt; baseline eGFR. Median age was 80 [interquartile range 75–83] years, pre- and postinterventional TR grades were 4 [3–5] and 1 [1–2], baseline eGFR was 36 [30–53] ml/min and New Yeark Heart Association (NYHA) IV was evident in 15% of patients. Multiple logistic regression analysis revealed TR vena contracta reduction (Odds ratio (OR) 1.35 [95% CI: 1.12–1.64] per mm, p = 0.002) and reduced preinterventional tricuspid annular plane systolic excursion (TAPSE) [OR 0.89 (95% CI: 0.79–0.99) per mm, p = 0.033] to independently predict renal improvement at FU. An eGFR improvement threshold of &gt;9 ml/min was associated with reduced 1-year heart failure hospitalization rates [adjusted hazard ratio 0.22 (95% CI: 0.07–0.62) p = 0.005].ConclusionEffective tricuspid edge-to-edge repair is associated with improved renal function and reduced heart failure hospitalization. In patients without renal improvement at 3-months follow-up, residual tricuspid regurgitation should be reevaluated for reintervention.
AI-enabled detection of QRS fragmentation from 12-lead electrocardiogram and its clinical relevance for predicting malignant arrhythmia onset
Authors: Ingelaere Sebastian , Villa Amalia , Varon Carolina , Van Huffel Sabine , Vandenberk Bert , Willems Rik 
Published: October 2024

BackgroundElectrocardiographic markers differentiating between death caused by ventricular arrhythmias and non-arrhythmic death could improve the selection of patients for implantable cardioverter-defibrillator (ICD) implantation. QRS fragmentation (fQRS) is a parameter of interest, but subject to debate. We investigated the association of an automatically quantified probability of fragmentation with the outcome in ICD patients.MethodsFrom a single-center retrospective registry, all patients implanted with an ICD between January 1996 and December 2018 were eligible for inclusion. Patients with active pacing were excluded. From the electronical medical record, clinical characteristics at implantation were collected and a 12-lead ECG was exported and analyzed by a previously validated machine-learning algorithm to quantify the probability of fQRS. To compare fQRS(+) and fQRS(−) patients, dichotomization was performed using the Youden index. Patients with a high probability of fragmentation in any region (anterior, inferior or lateral), were labeled fQRS(+). The impact of this fQRS probability on outcomes was investigated using Cox regression.ResultsA total of 1,242 patients with a mean age of 62.6 ± 11.5 years and a reduced left ventricular ejection fraction of 31 ± 12% were included of which 227 (18.3%) were female. The vast majority suffered from ischemic heart disease (64.3%) and were implanted in primary prevention (63.8%). 538 (43.3%) had a high probability of fragmentation in any region. Patients with a high probability of fragmentation had more frequently dilated cardiomyopathy (39.4% vs. 33.0%, p = 0.019), left bundle branch block (40.8% vs. 32.5%, p = 0.006) and a higher use of cardiac resynchronization therapy with defibrillator (CRT-D) devices (33.9% vs. 26.3%, p = 0.004). After adjustment in a multivariable Cox model, there was no significant association between the probability of global or regional fQRS and appropriate ICD therapy, inappropriate shock and short- or long-term mortality.ConclusionThere was no association between the automatically quantified probability of the presence of fQRS and outcome. This lack of predictive value might be due to the algorithm used, which identifies only the presence but not the severity of fragmentation.
Single center experience in localization of insulinoma by selective intraarterial calcium stimulation angiography - a case series of 15 years
Authors: Halmi Sándor , Berta Eszter , Diószegi Ágnes , Sira Lívia , Fülöp Péter , Nagy Endre V. , Győry Ferenc , Kanyári Zsolt , Tóth Judit , Bhattoa Harjit Pal , Bodor Miklós 
Published: October 2024

BackgroundInsulinomas are rare insulin-secreting neuroendocrine neoplasms of the pancreas. First-line treatment is the surgical removal of the tumor, however, the localization with standard imaging techniques is often challenging. With the help of selective intraarterial calcium stimulation the insulinoma’s localization can be narrowed down to one third of the pancreas which the selected artery supplies.ObjectiveWe aimed to prove the usefulness of the calcium stimulation test in case of 9 patients treated between 2006 and 2021 diagnosed with endogenous hyperinsulinemic hypoglycemia confirmed by fasting test, where conventional imaging methods, like transabdominal ultrasound, CT or MRI failed to detect the source of hyperinsulinemia.MethodsWe performed selective intraarterial calcium stimulation with angiography with calcium gluconate injected to the main supporting arteries of the pancreas (splenic, superior mesenteric and gastroduodenal arteries); blood samples were obtained from the right hepatic vein before, and 30, 60 and 120 seconds after calcium administration.ResultsWith selective angiography we found a significant elevation of insulin levels taken from the right hepatic vein in five of the nine cases. On histopathology, the lesions were between 1-2 cm, in one case malignancy was also confirmed. In four patients we found a significant rise of insulin levels obtained from all catheterized sites, which confirmed the diagnosis of nesidioblastosis. In three cases no surgery was performed, and the symptoms relieved with medical treatment.ConclusionsSelective intraarterial calcium stimulation remains an important tool in localization of the source of insulin excess, especially in cases where other diagnostic modalities fail.
U-shaped association between dietary niacin intake and chronic kidney disease among US elderly: a nationwide cross-sectional study
Authors: Xie Zhouzhou , Peng Shansen , Ou Gejun , Zhou Xiaoqi , Zhang Guihao , Jiang Huiming , Zhang Tianhui , Chen Nanhui 
Published: October 2024

BackgroundIn addition to hypertension or diabetes, elderly people are also considered one of the high-risk groups for chronic kidney disease (CKD). Although niacin is recognized for its renal protective properties, the link between dietary niacin intake and CKD remains uncertain. This study investigated this relationship in the elderly.MethodsWe included participants aged 60 and older from the National Health and Nutrition Examination Survey (NHANES) for the years 2003-2018. Dietary niacin intake was assessed through two non-consecutive 24-hour dietary recalls. CKD was diagnosed in individuals with a urine albumin-to-creatinine ratio exceeding 30 mg/g or an estimated glomerular filtration rate below 60 mL/min per 1.73 m^2. The study cohort comprised 4,649 participants, 1,632 of whom had CKD. Propensity score matching (PSM) was utilized to adjust for baseline differences between the groups.ResultsOur analysis, using smooth curve fitting and generalized additive models both before and after PSM, found a U-shaped curve depicting the relationship between dietary niacin intake and CKD risk, confirmed by a log-likelihood ratio test (P &lt; 0.05). Threshold effect analysis (after PSM) indicated a reduced risk of CKD in older adults with a niacin intake below 38.83 mg per day [odds ratio (OR) = 0.99, 95% confidence interval (CI) 0.97-1.00]. In contrast, higher intake levels significantly increased the risk (OR = 1.03, 95% CI 1.00-1.06). Subgroup analysis indicated that these associations were consistent across different stratification variables (P for interaction &gt; 0.05).ConclusionOur findings suggested a U-shaped association between dietary niacin intake and CKD risk among older Americans. However, further prospective cohort studies are needed to confirm this finding.
Complex factors combinations driving tea growers to adopt ecological agricultural practices in tea gardens
Authors: Yan Zhaoshuo , Zhang Wenzhe , Liu Xiao , Li Zhengyuan , Zhao Yutao , Zeng Mingjiang , Gao Shuilian 
Published: October 2024

IntroductionThe implementation of ecological agriculture practices in Chinese tea gardens plays a vital role in mitigating N2O emissions and addressing environmental degradation. Nevertheless, a dearth of discourse exists regarding the intricacies surrounding farmers' adoption of tea garden ecological agriculture practices (TGEAP), particularly the complex interplay between adoption factors and outcomes.MethodsUsing data of 310 farmers, this study employed complexity theories and Stimulus-Organism-Response theories, and integrated Partial Least Squares Structural Equation Modeling and fuzzy-set Qualitative Comparative Analysis to explore the complex relationships between farmer characteristics, internal and external factors, and adoption of farmer.ResultsThe results show three influential paths in the Stimulus-Organism-Response model, and environmental protection attitude (EPA) and production expectation (PE) act as intermediaries. Notably, EPA exhibits a masking effect in one pathway. These paths linked closely to three farmer characteristics. Beyond the Stimulus-Organism-Response model, nine combinations lead to farmers' adoption, and four to non-adoption.DiscussionWe discover scenarios where opposing environmental states or age lead to adoption, explaining the masking effect. These combinations highlight how a favorable environment influences both adoption and non-adoption. We also discuss other combinations that lead to adoption or non-adoption. The study suggests that governments employ targeted incentives to facilitate tea farmers' transition in agriculture.
Clones reactive to apoptotic cells and specific chemical adducts are prevalent among human thymic B cells
Authors: Hertel Andrea , Aguiar Talita , Mashiko Shunya , Núñez Sarah , Moore Carolina , Gao Baoshan , Ausmeier Mattea , Roy Poloumi , Zorn Emmanuel 
Published: October 2024

IntroductionThymus resident B cells were described more than 40 years ago. In early human life, these cells are found predominantly in the medulla and overwhelmingly display an unswitched IgM+ phenotype. The reactivity of thymic IgM B cells, however, is still unclear.MethodsHere, we generated 120 IgM-producing B cell clones from 3 separate thymus specimens obtained from infant, adolescent, and adult donors. Using flow cytometry and a unique high-dimensional ELISA platform, we investigated the clones’ reactivity to apoptotic cells as well as to common chemical adducts exposed on modified amino acids and other macromolecules.ResultsRegardless of the age, approximately 30-40% of thymic IgM B cells reacted to apoptotic cells. Further, 30-40% displayed reactivity to at least one adduct, including malondialdehyde, Homocysteine, and NEDD 8. Four distinct reactivity patterns were identified through this profiling. Notably, a significant association was observed between reactivity to apoptotic cells, and to one or more adducts, suggesting that the same determinants were recognized in both assays. Additionally, thymic IgM B cells reactive to adducts were more likely to recognize intra-nuclear or intra-cytoplasmic structures in Hep-2 cells as revealed by immunofluorescence staining.Conclusion/DiscussionCollectively, our findings suggest that thymic IgM B cells actively uptake apoptotic bodies and cellular debris in the medulla by binding specific chemical adducts. This mechanism could underpin their antigen-presenting function and further support their role in T-cell negative selection.
Coagulation-related genes for thyroid cancer prognosis, immune infltration, staging, and drug sensitivity
Authors: Sun Yuxiao , Zhang Yifei , Yang Yuchuan , Liu Weihao , Yin Detao 
Published: October 2024

IntroductionThyroid cancer (THCA) is the most common endocrine tumor. Coagulation may be associated with the development of cancer, but its role in THCA patients is not yet clear.MethodsIn this study, we determined the predictive value of coagulation biomarker D-dimer for THCA patient lateral lymph node metastasis (LLNM) through receiver operating characteristics (ROC) analysis and logistic regression analysis. Subsequently, this study used the TCGA database to identify coagulation-related molecular subtypes through consensus clustering analysis and compared their prognosis. We identified coagulation-related genes (CRGs) associated with prognosis in thyroid cancer through gene expression data and clinical information, and constructed a prognostic model by selecting the prognostic CRGs using LASSO regression. Patients were divided into high-risk and low-risk groups based on the median score. Subsequently, prognosis, clinical characteristics, gene mutation occurrence, immune infiltration, function, and drug sensitivity of the two groups were analyzed. We also constructed a nomogram combining the model and clinical features. Finally, the expression of the prognostic CRGs was validated by RT-qPCR.ResultsD-dimers had better performance in predicting LLNM(the area under the curve was 0.656 (95% CI 0.580-0.733), with a cut-off value of 0.065 mg/l), and D-dimer&gt;0.065mg/l was an independent predictor of LLNM. Then, we selected 8 prognostic CRGs to construct a predictive model. The prognosis of low-risk group patients was significantly better than that of high-risk group (P&lt;0.001). The results showed significant differences in clinical characteristics, gene mutation occurrence, immune infiltration, function, and drug sensitivity between the high-risk and low-risk groups. We validated by qPCR that these 8 prognostic CRGs were overexpressed in THCA cell lines.DiscussionOverall, this study provided an in-depth exploration of the potential role of the coagulation in thyroid cancer and its clinical significance, offering a new theoretical basis and research direction for personalized therapy and prognostic evaluation.
Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren’s syndrome via suppression of the CX3CL1/CX3CR1 axis
Authors: Yang Zi , Liu Mangnan , Chang Zhichao , Du Conglin , Yang Yang , Zhang Chen , Hu Liang 
Published: October 2024

IntroductionPrimary Sjögren syndrome (pSS) is a systemic autoimmune disease that is characterized by the infiltration of immune cells into the salivary glands. The re-establishment of salivary glands (SGs) function in pSS remains a clinical challenge. Myeloid-derived growth factor (MYDGF) has anti-inflammatory, immunomodulatory, and tissue-functional restorative abilities. However, its potential to restore SGs function during pSS has not yet been investigated.MethodsNonobese diabetic (NOD)/LtJ mice (pSS model) were intravenously administered with adeno-associated viruses carrying MYDGF at 11 weeks of age. Salivary flow rates were determined before and after treatment. Mice were killed 5 weeks after MYDGF treatment, and submandibular glands were collected for analyses of histological disease scores, inflammatory cell infiltration, PCR determination of genes, and Western blotting of functional proteins. Furthermore, mRNA sequencing and bioinformatics were used to predict the mechanism underlying the therapeutic effect of MYDGF.ResultsTreatment of NOD/LtJ mice with MYDGF alleviated pSS, as indicated by increased salivary flow rate, reduced lymphocyte infiltration, attenuated glandular inflammation, and enhanced AQP5 and NKCC1 expression. The gene expression levels of cytokines and chemokines, including Ccl12, Ccl3, Il1r1, Ccr2, Cx3cr1, Il7, Mmp2, Mmp14, Il1b, and Il7, significantly decreased after treatment with MYDGF, as determined by RNA sequencing. Meanwhile, MYDGF inhibits infiltration of macrophages (Mϕ) in SGs, induces polarization of M2ϕ, and suppresses C-X3C motif ligand 1 (CX3CL1)/C-X3C motif receptor 1 (CX3CR1) axis.ConclusionsOur findings showed that MYDGF could revitalize the SGs function of pSS, inhibit infiltration of Mϕ, and promote M2ϕ polarization via suppression of the CX3CL1/CX3CR1 axis, which has implications for potential therapy for pSS.
Ubiquitin-proteasome system in Plasmodium: a potential antimalarial target to overcome resistance – a systematic review
Authors: Gonçalves Adriana F. , Lima-Pinheiro Ana , Ferreira Pedro E. 
Published: October 2024

BackgroundMalaria is a devasting parasitic disease that causes over half a million deaths every year. The necessity for prompt and thorough antimalarial drug discovery and development is accelerated by the rise in multidrug resistance and the lack of an effective vaccine. The Plasmodium spp. proteasome represents a prospective target for antimalarial treatment since several chemotherapy types have been shown to potently and selectively limit the growth of parasites. Combined with first-line artemisinin medicines, it creates synergy, even in the artemisinin-resistant parasites.MethodsPRISMA guidelines were used in the development of this systematic review. A literature search was performed in March 2024 in PubMed, Science Direct, and Scopus databases, with the following keywords: ((antimalarial resistance) AND (plasmodium OR malaria) AND (proteasome)) NOT (cancer [Title/Abstract]). Only articles with the susceptibility assessment were included.ResultsHerein, 35 articles were included in the systematic review, which was divided into two subcategories: those that studied the UPS inhibitors, which accounted for 25 articles, and those that studied genetic modifications, including knockouts, knockdowns, and mutations, in the UPS toward antimalarial resistance, accounting for 16 articles. 6 articles included both subcategories. In total, 16 categories of inhibitors were analyzed, together with two knockdowns, one knockout, and 35 mutations.ConclusionIn this study, we reviewed the literature for available inhibitors and their respective susceptibility and ability to develop resistance toward Plasmodium spp. 26 s proteasome. The proteasome was highlighted as a potential antimalarial target and as an artemisinin partner drug. However, host toxicity and susceptibility to resistance appear as the main obstacle in the development of highly potent drugs, indicating a need for additional scrutiny during any further drug development efforts.
Association of hearing loss and risk of depression: a systematic review and meta-analysis
Authors: Wei Jingxuan , Li Yang , Gui Xiongbin 
Published: October 2024

BackgroundPrevious studies have suggested a possible link between hearing loss and the risk of depression. However, the strength of this association remains uncertain across varying follow-up periods, age groups, cohort studies, and longitudinal study designs.MethodsWe conducted an extensive literature search across PubMed, Embase, and the Cochrane Library databases to retrieve pertinent studies. The quality of observational studies was assessed using the Newcastle–Ottawa Scale. Synthesis and meta-analysis of odds ratios (ORs) along with corresponding 95% confidence intervals (CIs) were performed using Stata 14.0. Funnel plot analysis and Egger’s regression test were utilized to assess potential publication bias.ResultsThis meta-analysis comprised 24 cohort studies conducted between 2007 and 2024, with sample sizes ranging from 548 to 254,466 participants. Among these participants, 24,304 had experienced depression events. The pooling analysis shows that hearing loss is associated with an increased risk of depression (OR = 1.35; 95%CI: 1.27–1.44). In the subgroup analysis, the retrospective cohort exhibited a slightly higher risk of depression compared to the prospective cohort (OR = 1.43; 95% CI: 1.30–1.58). There are differences in the risk of depression among young, middle-aged, and older individuals, with older adults facing a higher risk (OR = 1.33, 95% CI: 1.21–1.45). Additionally, the risk of depression was slightly higher in the sudden sensorineural hearing loss (SSNHL) group compared to the non-SSNHL group (OR = 1.62; 95% CI: 1.27–2.07). Furthermore, in cohorts with a follow-up time ≥ 5 years, the risk of depression was higher compared to those with &lt;5 years of follow-up (OR = 1.39; 95% CI: 1.26–1.54).ConclusionOur meta-analysis shows that hearing loss increases the risk of depression. These findings provide evidence that hearing loss should be recognized as an independent risk factor for depression.Clinical trial registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42024502043.
Borehole disposal of spent fuel and other high-level wastes: the case for deep, vertical, fully cased holes in saturated “hard” rock
Authors: Gibb Fergus , Beswick John , Travis Karl 
Published: October 2024

Driven by major advances in deep drilling technology and the geological understanding of the deep continental crust over the past 70 years, disposal in deep boreholes has moved from being technically unachievable to the point that it now offers a viable solution for the most hazardous nuclear wastes that could effectively be implemented “tomorrow”—i.e., within a few years. Moreover, disposal in deep boreholes is arguably superior in almost every respect to the mined and engineered repositories being pursued for high level waste by most countries. During the first 50 years of their evolution, almost all deep borehole disposal concepts shared five key aspects: (i) the hole was as deep as possible, (ii) it was vertical, (iii) it was fully cased, and (iv) it was in “hard” basement rock (v) saturated with aqueous fluid (groundwater). Technical advances in drilling over the last 20 years have encouraged proposed versions of the concept which depart from one or more of these aspects, but it is our contention that all five fundamental aspects should be retained. This paper summarises the more important arguments supporting this view. In order to meet the necessary post-closure (radiological) safety requirements, engineer out possible operational problems during construction and waste-package deployment, and capitalise on the main benefits of borehole disposal, the hole itself must be over 3 km deep, vertical, fully cased, and in suitably hard (ideally granitic) host rock saturated with aqueous fluid.