bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells
Authors: Shishido Yurina , Baba Takashi , Sato Tetsuya , Shima Yuichi , Miyabayashi Kanako , Inoue Miki , Akiyama Haruhiko , Kimura Hiroshi , Kanai Yoshiakira , Ishihara Yasuhiro , Haraguchi Shogo , Miyazaki Akira , Rozman Damjana , Yamazaki Takeshi , Choi Man-Ho , Ohkawa Yasuyuki , Suyama Mikita , Morohashi Ken-ichirou 
Published: February 2017

AbstractSRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells. Lactate and cholesterol metabolisms, essential for nursing the developing germ cells, were down-regulated in XX/Sry cells, which appears to be caused at least in part by the differential expression of histone modification enzymes SMCX/SMCY (H3K4me3 demethylase) and UTX/UTY (H3K27me3 demethylase) encoded by the sex chromosomes. We suggest that down-regulation of lactate and cholesterol metabolism that may be due to altered epigenetic modification affects the nursing functions of XX/Sry Sertoli cells.
Biochemical Genetics of Fundulus heteroclitus (L.): V. Inheritance of 10 Biochemical Loci
Authors: Brown D. C. , Ropson I. J. , Powers D. A. 
Published: February 2017

Abstract
Starch gel electrophoresis has shown that natural populations of Fundulus heteroclitus have electrophoretic variants for at least 21 loci. We provide inheritance data for 10 polymorphic systems: esterases (Est-B, EST-C, and Est-D); aspartate amlnotransferases (Aat-A, and Aat-B); mannosephosphate isomerase (Mpl-A); acid phosphatase (Ap-A); phosphoglucomutase (Pgm-B); hexose-6-phosphate dehydrogenase (H6pdh-A); and fumarase (Fum-A). Variants for nine of these loci segregate as autosomally inherited codominant alleles. The other system, EST-C, does not reflect such inheritance. We have identified two possible linkage groups: H6pdh-A may be loosely linked to Pgm-B, and Fum-A appears to be linked to Pgm-A. Tissue specificity and intracellular localization for all these loci are also presented.
Cerebellar volume in autism: Meta-analysis and analysis of the ABIDE cohort
Authors: Traut Nicolas , Beggiato Anita , Bourgeron Thomas , Delorme Richard , Rondi-Reig Laure , Paradis Anne-Lise , Toro Roberto 
Published: February 2017

AbstractCerebellar volume abnormalities have been often suggested as a possible endophenotype for autism spectrum disorder (ASD). We aimed at objectifying this possible alteration by performing a systematic meta-analysis of the literature, and an analysis of the Autism Brain Imaging Data Exchange (ABIDE) cohort. Our meta-analysis sought to determine a combined effect size of ASD diagnosis on different measures of the cerebellar anatomy, as well as the effect of possible factors of variability across studies. We then analysed the cerebellar volume of 328 patients and 353 controls from the ABIDE project. The meta-analysis of the literature suggested a weak but significant association between ASD diagnosis and increased cerebellar volume (p=0.049, uncorrected), but the analysis of ABIDE did not show any relationship. The studies in the literature were generally underpowered, however, the number of statistically significant findings was larger than expected. Although we could not provide a conclusive explanation for this excess of significant findings, our analyses would suggest publication bias as a possible reason. Finally, age, sex and IQ were important sources of cerebellar volume variability, however, independent of autism diagnosis.
Compaction of Duplex Nucleic Acids upon Native Electrospray Mass Spectrometry
Authors: Porrini Massimiliano , Rosu Frédéric , Rabin Clémence , Darré Leonardo , Gómez Hansel , Orozco Modesto , Gabelica Valérie 
Published: February 2017

ABSTRACTNative mass spectrometry coupled to ion mobility spectrometry is a promising tool for structural biology. Intact complexes can be transferred to the mass spectrometer and, if native conformations survive, collision cross sections give precious information on the structure of each species in solution. Based on several successful reports for proteins and their complexes, the conformation survival becomes more and more taken for granted. Here we report on the fate of nucleic acids conformation in the gas phase. Disturbingly, we found that DNA and RNA duplexes, at the electrospray charge states naturally obtained from native solution conditions (≥ 100 mM aqueous NH4OAc), are significantly more compact in the gas phase compared to the canonical solution structures. The compaction is observed for short (12-bp) and long (36-bp) duplexes, and for DNA and RNA alike. Molecular modeling (density functional calculations on small helices, semi-empirical calculations on up to 12-bp, and molecular dynamics on up to 36-bp duplexes) demonstrates that the compaction is due to phosphate group self-solvation prevailing over Coulomb-driven expansion. Molecular dynamics simulations starting from solution structures do not reproduce the experimental compaction. To be experimentally relevant, molecular dynamics sampling should reflect the progressive structural rearrangements occurring during desolvation. For nucleic acid duplexes, the compaction observed for low charge states results from novel phosphate-phosphate hydrogen bonds formed across both grooves at the very late stages of electrospray.
Public-private partnership in university female students’ hostel delivery
Authors: Babatunde Solomon Olusola , Perera Srinath 
Published: February 2017

Purpose
Although studies on student residential satisfaction abound, yet there are limited studies conducted on students’ satisfaction in respect of hostel accommodation facilities undertaken through public-private partnerships (PPPs) in Nigeria. The purpose of this study is to assess the level of students’ satisfaction with the provision of university hostel accommodation facility using build-operate-transfer (BOT) model at University of Lagos (Unilag), Nigeria.

Design/methodology/approach
The study adopted literature review and questionnaire survey within the case study of a BOT hostel accommodation facility at Unilag in Nigeria. The students who reside at the case study were the respondents. Hence, 153 self-administered questionnaires were purposively distributed face-to-face to the entire student population that live in the BOT hostel. Thus, a total of 96 completed questionnaires representing 63 per cent were collected and found suitable for the analysis. The data collected were analysed using the mean score and chi-square (χ2) test.

Findings
The study revealed a total mean satisfaction level of 3.46 (on a 5-point Likert scale), which indicates that students are “somehow satisfied” with the university hostel accommodation facility undertaken through BOT. The study further established that age and level of students have no significant influence on the overall students’ satisfaction with their hostel accommodation facility undertaken through BOT.

Practical implications
The study would provide valuable feedback to private investors, university authorities and facility managers of higher education institutions to improve their services in the near future.

Originality/value
The identified variables that have tested to be significant in assessing students’ level of satisfaction in BOT hostel accommodation facilities could be used to assess users’ satisfaction in similar facilities delivered through PPPs in other countries.
Developing a research framework for the green maintainability of buildings
Authors: Chew M.Y.L. , Conejos Sheila , Asmone Ashan Senel 
Published: February 2017

Purpose
The aim of this paper is to present a research framework for the green maintainability of buildings. This study makes the case for the development of a new concept called “green maintainability”. The paper also identifies and discusses the knowledge gap concerning green facilities management (FM). As an integral part of green FM, the economic, environmental and social impacts and opportunities of green maintainability throughout the total life cycle of the facility are also highlighted.

Design/methodology/approach
The little attention paid to the maintainability of green buildings has resulted in losses of lives due to occupational health and safety hazards as well as high operation and maintenance costs. To address this issue, this study has conducted a literature review to determine the relevant background knowledge and provides a conceptual framework that will aid in conceptualizing the green maintainability of buildings and the development of a research framework for the furtherance of this concept.

Findings
This paper finds that there is little research on the maintainability of green buildings, and the studies about the maintainability of green features are nonexistent in current research. This study confirms the knowledge gap of this little-researched area and draws from it the formulation of a research framework for the green maintainability of buildings to ensure green FM. Emerging literature on green practices and methods is currently receiving attention from academia, as well as building and construction practitioners, and can valuably contribute to the existing theories, practices and methods concerning building maintainability and facilities management.

Originality/value
This study develops the novel concept of green maintainability, which integrates maintainability and green FM at the planning/design stage. The proposed research framework is the first attempt to investigate the green maintainability of different typologies of buildings and especially green building technologies.
Call centers: is there an upside to the dissatisfied customer experience?
Authors: Hudson Sarah , González-Gómez Helena V. , Rychalski Aude 
Published: February 2017

PurposeThis paper aims to present the triggers of negative customer emotions during a call center encounter and the impact of emotions on satisfaction and loyalty. It suggests ways of mitigating the negative effects of such emotions.Design/methodology/approachThis paper uses exploratory research consisting of 33 focus groups with 121 narratives of a call center encounter.FindingsCallers predominantly report frustration as the emotion arising from negative experiences in a call center encounter. Goal urgency, reduced customer control and uncertainty underlie this emotional experience. Triggers include assessments of “dehumanized”, “incompetent” or “hostile” call center employees as well as the more well-known multiple transfers and waiting time. Customer may remain loyal after a frustrating encounter if they believe that alternative services will be no better.Research limitations/implicationsDisembodied service encounters generate conditions of reduced control and certainty which foster negative emotions. The outcomes of negative emotions are not always negative if the call center context is managed appropriately. Focus groups took place in a European business school, so generalizability of the results to other regions may be limited.Practical implicationsNegative emotions can have a strong effect on loyalty, a key issue in service organizations. This paper provides insights into how to manage customer emotions effectively.Originality/valueCustomer satisfaction and loyalty in terms of emotions are generally overlooked in the call center industry because of the focus on performance metrics. This study shows that emotions must be taken into account to ensure customer retention and the competitive edge.
The charge of the white brigade
Authors: Hirsch Peter Buell 
Published: February 2017

Purpose
The purpose of this study is to examine the long struggle to increase diversity and inclusion in the leadership of large corporations. In spite of significant progress, women and minorities are still woefully under-represented. The extraordinary diversity of the Millennial generation (born between 1982 and 2004) should help accelerate change, but it is not believed that demographics on its own will dramatically improve matters. This viewpoint examines some of the underlying tensions around diversity in the workplace and suggests some approaches that could have a positive impact.

Design/methodology/approach
The paper cites a number of statistics from the secondary literature that demonstrate how, unfortunately, diversity still suffers periodic setbacks as well as improvements. It then examines the actual world of work to propose ways in which “inclusion” in the workplace and not just diversity in recruitment has a role to play.

Findings
In addition to the importance of a strong minority recruitment pipeline, the establishment of affinity networks and minority mentoring, the author also found that some routine business practices need to be carefully examined to see how they promote or defeat inclusion. These include the trend toward less formal meeting structures that can militate against diverse voices being heard; the need to create greater awareness around the tendency of men to interrupt women disproportionality; and the tyranny of teams in which individual credit is often obscured. By being conscious of these practices, their negative impact on diversity and inclusion can be mitigated.

Research limitations/implications
The findings are the author’s own viewpoint and would need to be validated in controlled studies.

Practical implications
The best practices proposed would need to be adapted to fit well in different corporate cultures both geographically and by industry type.

Social implications
If corporate leaders devoted sufficient attention to these day-to-day business practices, the author believes that they would see a noticeable increase in diversity and inclusion, leading to better productivity and more rewarding roles for women and minorities in the workplace.

Originality/value
While diversity and inclusion have been written about extensively, the author is not aware of any comparable reflections on best practices in the workplace of this kind.
Why understanding what matters to the patient matters
Authors: Patterson Karen D. 
Published: February 2017

Purpose
The purpose of this paper is to explore the evolving of integrated care, focussing on developments in why what matters to the patient matters and how to better understand the value the patient brings to and anticipates from integrated care.

Design/methodology/approach
A descriptive piece that draws on relevant research findings, literature, reports and health policy.

Findings
Although integrated care is a contemporary policy agenda, it is bringing back to the forefront the benefits of a population health planning approach. The shift in the health systems focus from inward looking to outward looking is promising, albeit has taken 25 years and is still evolving. Leadership is being demonstrated, however, a shared vision is elusive and not all voices are being heard. The development of a narrative that defines integrated care from the patient’s point of view is an important step. Next is better understanding the immediate and anticipatory public value integrated care offers.

Originality/value
This is a novel perspective of an enduring health reform policy concept. Currently the literature is thin.
Retrospective analysis of the clinical response of palmoplantar pustulosis after dental infection control and dental metal removal
Authors: Kouno Michiyoshi , Nishiyama Akihiro , Minabe Masaki , Iguchi Naohiko , Ukichi Kenichiro , Nomura Takeshi , Katakura Akira , Takahashi Shinichi 
Published: February 2017

AbstractBoth metal allergy and dental focal infection have been considered as causative factors for palmoplantar pustulosis, and several case reports described that the skin lesions were ameliorated after dental metal removal or dental infection control. However, limited data are available to evaluate the association of these factors with disease severity of palmoplantar pustulosis. This study is designed to analyze the clinical outcome of 85 palmoplantar pustulosis patients after dental infection control (n = 70), tonsillectomy (n = 6) and dental metal removal (n = 9). More than half of the patients (63%, 44/70) showed positive clinical outcome after dental infection control. The skin lesions of all patients with tonsillitis were improved after tonsillectomy (100%, 6/6). On the other hand, one‐third of patients (33%, 3/9) showed positive response after dental metal removal. These results suggest that focal infection is more closely associated with palmoplantar pustulosis than dental metal allergy. According to our findings, palmoplantar pustulosis patients should be preferentially examined for focal infections.
Application of COL1A1–PDGFB fusion gene detection by fluorescence in situ hybridization in biopsy tissue of dermatofibrosarcoma protuberans
Authors: Zhang Zhang , Chen Huijiao , Chen Min , He Xin , Wang Yiying , Zhang Hongying 
Published: February 2017

AbstractSeveral uncommon variants of dermatofibrosarcoma protuberans (DFSP) and the limitations of small biopsies render pathological diagnosis difficult. The aim of this study was to analyze the utility of fluorescence in situ hybridization (FISH) in the detection of the collagen type I‐α1/platelet derived growth factor‐β (COL1A1–PDGFB) fusion gene in biopsies of DFSP. Twenty‐three consecutive biopsy specimens of DFSP were reviewed for clinicopathological features and examined with the COL1A1–PDGFB fusion probe and PDGFB break‐apart probe using FISH analysis. The 23 tumor samples consisted of 11 males and 12 females (mean age at diagnosis, 37 years; range, 14–75 years). Eighteen conventional DFSP, one Bednar tumor, two myxoid DFSP and two fibrosarcomatous DFSP samples were included in the group. Strong and extensive CD34 expression was observed in 19 of 23 cases (83%). Twenty‐one cases (91%) were positive for both the COL1A1–PDGFB fusion signal and the PDGFB break‐apart signal. This is one of the few studies to demonstrate the value of FISH analysis of the COL1A1–PDGFB gene, which could validate complicated and suspected diagnoses in the routine biopsy of DFSP.
Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes
Authors: Thompson Luke R. , Nikolakakis Kiel , Pan Shu , Reed Jennifer , Knight Rob , Ruby Edward G. 
Published: February 2017

SummaryThe marine bacterium Vibrio fischeri is the monospecific symbiont of the Hawaiian bobtail squid, Euprymna scolopes, and the establishment of this association involves a number of signaling pathways and transcriptional responses between both partners. We report here the first full RNA‐Seq dataset representing host‐associated V. fischeri cells from colonized juvenile E. scolopes, as well as comparative transcriptomes under both laboratory and simulated marine planktonic conditions. These data elucidate the broad transcriptional changes that these bacteria undergo during the early stages of symbiotic colonization. We report several previously undescribed and unexpected transcriptional responses within the early stages of this symbiosis, including gene expression patterns consistent with biochemical stresses inside the host, and metabolic patterns distinct from those reported in associations with adult animals. Integration of these transcriptional data with a recently developed metabolic model of V. fischeri provides us with a clearer picture of the metabolic state of symbionts within the juvenile host, including their possible carbon sources. Taken together, these results expand our understanding of the early stages of the squid–vibrio symbiosis, and more generally inform the transcriptional responses underlying the activities of marine microbes during host colonization.
Developing models to predict early postoperative patient deterioration and adverse events
Authors: Petersen Tym Mitchell K. , Ludbrook Guy L. , Flabouris Arthas , Seglenieks Richard , Painter Thomas W. 
Published: February 2017

BackgroundAccurate identification of patients at risk of early postoperative deterioration allows needs‐based allocation of patients to appropriate levels of care. This study aimed to record the incidence of early postoperative deterioration and identify factors predictive of at‐risk patients. Doing so may assist future evidence‐based perioperative planning and allocation of patients to high‐acuity facilities.MethodsWith ethical approval, data from elective non‐cardiac surgical patients were collected between May and August 2013. Patient and surgical factors potentially related to postoperative deterioration were collected from preoperative assessment records. Data on deterioration in the postanaesthesia care unit (PACU), and on the wards were collected prospectively for a period of 72 h postoperatively. Patient factors, surgical factors and PACU events were compared with ward events using binomial logistic regression analysis.ResultsOf the 747 patients, postoperative deterioration was common both in PACU (155 (20.1%) patients) and on the wards (125 (16.7%)). Common ward events included hypotension (64 (8.2%)) and desaturation (59 (6.2%)). A rapid response team call occurred for 33 (4.4%) patients and an unplanned ICU admission for seven (0.9%) patients. A history of atrial fibrillation and chronic liver disease, duration of surgery and excessive sedation in PACU, among others, were strongly associated with subsequent ward deterioration. However, measures of surgical complexity were not.ConclusionsPatient factors, duration of surgery and events in PACU can be predictive of subsequent early postoperative ward clinical deterioration. Such information may aid appropriate perioperative decision‐making with respect to postoperative utilization of high‐acuity facilities.
Could habits hold the key to weight loss maintenance? A narrative review
Authors: Cleo G. , Isenring E. , Thomas R. , Glasziou P. 
Published: February 2017

AbstractDespite the significance placed on lifestyle interventions for obesity management, most weight loss is followed by weight regain. Psychological concepts of habitual behaviour and automaticity have been suggested as plausible explanations for this overwhelming lack of long‐term weight loss success. Interventions that focus on changing an individual's behaviour are not usually successful at changing an individual's habits because they do not incorporate the strategies required to break unhealthy habits and/or form new healthy habits. A narrative review was conducted and describes the theory behind habit formation in relation to weight regain. The review evaluated the effectiveness of using habits as tools to maintain weight loss. Three specific habit‐based weight loss programmes are described: ‘10 Top Tips’, ‘Do Something Different’ and ‘Transforming Your Life’. Participants in these interventions achieved significant weight loss compared to a control group or other conventional interventions. Habit‐based interventions show promising results in sustaining behaviour change. Weight loss maintenance may benefit from incorporating habit‐focused strategies and should be investigated further.