bluuebunny/crossref_metadata_2025_split huggingface dataset:
Childhood poverty, social support, immigration background and adolescent health and life satisfaction: A population‐based longitudinal study
Authors: Gill Randip , Karim Mohammad Ehsanul , Puyat Joseph H. , Guhn Martin , Petteni Monique Gagné , Oberle Eva , Janus Magdalena , Georgiades Katholiki , Gadermann Anne M. 
Published: October 2024

AbstractObjectivesThis study examined whether poverty (neighborhood and household) was associated with future health or life satisfaction outcomes and whether the association operated through social support (adult support at home, adult support at school, peer belonging), or differed by the immigration background (nonimmigrant family or immigrant family) of the family.MethodsThis study utilized a retrospective, longitudinal, population‐based cohort that included self‐reported survey data from the Middle Years Development Instrument (MDI) completed by children at age 9 and age 12, linked to administrative records. Participants included 5906 children in British Columbia, Canada. Neighborhood and household poverty were observed at age 8. Social support from adults and peers was self‐reported at age 9. Outcomes (overall health; life satisfaction) were self‐reported at age 12. Adjusted multi‐level multiple linear regression analyses and parallel mediation analyses were utilized. The interaction between poverty exposure and immigration background was also examined.ResultsExposure to either poverty type was associated with lower levels of life satisfaction and overall health at age 12, though household poverty appeared to be associated with lower outcomes in comparison to neighborhood poverty. The indirect effects of poverty on outcomes appeared to operate primarily through adult support at home and peer belonging. Children in immigrant families had a larger negative association between neighborhood poverty and life satisfaction.ConclusionsHousehold poverty had a larger negative association to outcomes in comparison to neighborhood poverty. The association of poverty to outcomes differed by immigration background and operated partially through adult support at home and peer belonging.
Dictionary Learning Method Based on K‐Sparse Approximation and Orthogonal Procrustes Analysis for Reconstruction in Bioluminescence Tomography
Authors: Su Linzhi , Chen Limin , Tang Wenlong , Gao Huimin , Chen Yi , Gao Chengyi , Yi Huangjian , Cao Xin 
Published: October 2024

ABSTRACTBioluminescence tomography (BLT) is one kind of noninvasive optical molecular imaging technology, widely used to study molecular activities and disease progression inside live animals. By combining the optical propagation model and inversion algorithm, BLT enables three‐dimensional imaging and quantitative analysis of light sources within organisms. However, challenges like light scattering and absorption in tissues, and the complexity of biological structures, significantly impact the accuracy of BLT reconstructions. Here, we propose a dictionary learning method based on K‐sparse approximation and Orthogonal Procrustes analysis (KSAOPA). KSAOPA uses an iterative alternating optimization strategy, enhancing solution sparsity with k‐coefficients Lipschitzian mappings for sparsity(K‐LIMAPS) in the sparse coding stage, and reducing errors with Orthogonal Procrustes analysis in the dictionary update stage, leading to stable and precise reconstructions. We assessed the method performance through simulations and in vivo experiments, which showed that KSAOPA excels in localization accuracy, morphological recovery, and in vivo applicability compared to other methods.
Biocompatible and Safe Decellularized Spinach With Antibacterial and Wound Healing Activity
Authors: Ksouri Rihab , Aksel Hamide , Saghrouchni Hamza , Saygideger Yasemin 
Published: October 2024

ABSTRACTCreating acellular vascularized constructs from animal and plant tissue is one of the well‐known strategies for scaffold assembly. Decellularization takes an important position among these strategies. The most common method is chemical decellularization. This approach employs high concentrations of detergents, primarily Triton X‐100, sodium dodecyl sulfate (SDS), and sodium hypochlorite (SH). In this work, novel techniques for decellularizing spinach were developed using detergents frequently utilized in laboratories. Spinach leaves were decellularized using Tween‐20, SDS, and SH at low concentrations to generate an acellular plant matrix for tissue engineering. We measured the quantities of DNA and protein, as well as the decellularization using hematoxylin and eosin (H&E) staining. The biocompatibility and capacity of the biostructures to stimulate fibroblast wound healing were assessed using MTT and the Scratch assay. The antibacterial activity of the scaffolds was also tested against a gram‐positive bacterium, Staphilococcus aureus, which is a common pathogen associated with wound healing. The best shape, evident vascularization, and good biocompatibility were seen in the Tween‐20 decellularized samples at 1% concentration at 21°C and 37°C through the enhancement of cell proliferation and wound healing. In terms of antibacterial activity, all scaffold samples had a significant effect on Staphilococcus aureus, where the number of bacterial colonies in all six scaffold groups became zero after 4 h of treatment. The scaffolds also showed a 100% kill rate on Staphilococcus aureus, which could avoid wound infection during the repair process, and that can be suggested as a scaffold for tissue engineering applications and an important constituent for pharmacological activities.
Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct
Authors: Costello Lydia , Goncalves Kirsty , De Los Santos Gomez Paola , Hulette Ben , Dicolandrea Teresa , Flagler Michael J. , Isfort Robert , Oblong John , Bascom Charlie , Przyborski Stefan 
Published: October 2024

AbstractIncreased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming commonplace due to initiatives and the cost of clinical testing. In this study, we utilise a well‐defined and characterised bioengineered skin construct as a tool to investigate the cellular and molecular dynamics involved in skin ageing from a dermal perspective. Through incorporation of ageing fibroblasts into the dermal compartment we demonstrate the significant impact of dermal‐epidermal crosstalk on the overlying epidermal epithelium. We characterise the paracrine nature of dermal‐epidermal communication and the impact this has during skin ageing. Soluble factors, such as inflammatory cytokines released as a consequence of senescence associated secretory phenotype (SASP) from ageing fibroblasts, are known to play a pivotal role in skin ageing. Here, we demonstrate their effect on epidermal morphology and thickness, but not keratinocyte differentiation or tissue structure. Through a novel in vitro strategy utilising bioengineered tissue constructs, this study offers a unique reductionist approach to study epidermal and dermal compartments in isolation and tandem.
Evaluation of Anterior and Middle Brain Structures With Cerebrovascular Flow in Fetuses With Fetal Growth Restriction: A Prospective Study
Authors: Ulusoy Can Ozan , Ağaoğlu Recep Taha , Sucu Serap Topkapi , Kurt Dilara Sarikaya , Bucak Mevlüt , Şeyhanli Zeynep , Yücel Kadriye Yakut 
Published: October 2024

ABSTRACTObjectiveTo investigate the adaptation of the anterior cerebral artery (ACA) in fetuses with fetal growth restriction (FGR) and assess if forebrain and midbrain structures are affected by vascular adaptations.MethodsA prospective case–control study involving normally developed fetuses and those with late‐onset FGR (estimated fetal weight < 3rd percentile and/or abdominal circumference < 3rd percentile). Doppler indices of the middle cerebral artery (MCA), ACA and umbilical artery (UA) were determined between 32 + 0 and 37 + 0 weeks. Neurosonography assessed the depth of the insula, the sylvian fissure, and the antero‐posterior diameter of the frontal lobes (FAPD).ResultsThe cerebral‐placental ratio (CPR) and cerebro‐placental‐uterine ratio (CPUR) were lower in FGR cases. ACA PI percentile values were significantly lower in the FGR group (p = 0.020). Sylvian fissure depth was significantly lower in FGR fetuses.ConclusionThe ACA may be the first cranial vascular structure affected in fetuses with FGR. This may be related to the impact on postnatal cognitive functions in FGR patients.
Trial Registration: NCT06215690
Clock Gene Expression in Eel Retina and Hypothalamus: Response to Photoperiod and Moonlight
Authors: Hyeon Ji‐Yeon , Byun Jun‐Hwan , Kim Byeong‐Hoon , Hettiarachchi Sachithra Amarin , Han Jeonghoon , Choi Young‐Ung , Noh Choong‐Hwan , Takeuchi Yuki , Choi Soo‐Youn , Park Jong‐Eun , Hur Sung‐Pyo 
Published: October 2024

ABSTRACTAssessment of the clock genes, Period (Per) 1, Per2, Per3, and Cryptochrome (Cry) 2, Cry3, and Cry4, can help better understand eel spawning ecology. In this study, the circadian rhythm and moonlight effects of these clock genes in the eel retina and hypothalamus were analyzed. We examined clock gene expression patterns under 12 h light:12 h darkness (12L12D), constant darkness (DD), and constant light (LL) conditions; under short photoperiod (SP; 9L15D) and long photoperiod (LP; 15L9D), and during the new moon (NM) and full moon in male eels. Per2 expression increased after sunrise, Cry2, and Cry4 expression increased around sunset, and Per1, Per3, and Cry3 expression increased before sunrise. Under SP conditions, oscillations of retinal Per3 and Cry4, which did not occur under LP conditions, were generated. In addition, retinal Cry4 oscillation was generated under NM conditions. These results suggest that the retina of the eel may play an important role in regulating circadian rhythm, and migration is initiated by the synchronization of clock genes by moonlight, suggesting that photic signals are closely related to the migratory activity of the eel.
The suppression of OTUD7B by miR‐491‐5p enhances the ubiquitination of VEGFA to suppress vascular mimicry in non‐small cell lung cancer
Authors: Chen Xiaofei , He Lijun , Zhong Hai , Yan Chenxin , Ke Bin , Shi Lin 
Published: October 2024

AbstractBackgroundNon‐small cell lung cancer (NSCLC) is the main type of lung cancer with high morbidity and mortality. Vascular mimicry (VM), a distinct microcirculation model in tumors that differs from classical angiogenesis, is strongly associated with poor clinical outcomes in cancer patients. miR‐491‐5p has been reported to prevent NSCLC progression, including proliferation, metastasis, and angiogenesis. However, the effect and mechanism of miR‐491‐5p on VM have not been studied in NSCLC.MethodsThe expression of miR‐491‐5p was detected by quantitative reverse transcription PCR (qPCR) and fluorescence in situ hybridization (FISH). Cell counting kit‐8 (CCK‐8) and 5‐ethynyl‐2′‐deoxyuridine (EdU) staining assays were used to examine cell growth. Tube formation assay was used to assess VM in NSCLC cells. Immunohistochemistry (IHC) and western blot were performed to detect protein expression. Immunoprecipitation was used to confirm the interaction between OTU deubiquitinase 7B (OTUD7B) and vascular endothelial growth factor A (VEGFA), and the level of ubiquitinated VEGFA. A nude mouse tumorigenesis model was used to evaluate the carcinogenic capacity of NSCLC cells in vivo. Luciferase reporter assay was used to identify the potential target of miR‐491‐5p.ResultsMiR‐491‐5p was found downregulated in NSCLC tissues, and miR‐491‐5p deficiency was strongly associated with angiogenesis. miR‐491‐5p mimics suppressed cell viability, migration, and VM. Conversely, an inhibitor of miR‐491‐5p had the opposite effect. OTUD7B, a deubiquitinase, was identified as a downstream target of miR‐491‐5p. A luciferase reporter assay indicated that miR‐491‐5p directly binds to the 3′UTR of OTUD7B. Moreover, mimics of miR‐491‐5p caused a significant reduction in the OTUD7B protein in NSCLC cells, and an inhibitor of miR‐491‐5p stabilized the OTUD7B protein. In addition, overexpression of OTUD7B promoted cell proliferation, migration, and VM, similar to the effects of an inhibitor of miR‐491‐5p. Further exploration revealed that OTUD7B interacts with VEGFA and that the miR‐491‐5p‐OTUD7B axis modulates the ubiquitination of VEGFA. The rescue experiment indicated that OTUD7B compromised the inhibitory effects of miR‐491‐5p on the cellular function of NSCLC cells.ConclusionsOverall, our study first proved that miR‐491‐5p impedes VM by suppressing OUTD7B and promoting the ubiquitination of VEGFA. The miR‐491‐5p/OTUD7B axis may be a novel target for antiangiogenic therapy in NSCLC.
Basilica: New canonical decomposition in matching theory
Authors: Kita Nanao 
Published: October 2024

AbstractIn matching theory, one of the most fundamental and classical branches of combinatorics, canonical decompositions of graphs are powerful and versatile tools that form the basis of this theory. However, the abilities of the known canonical decompositions, that is, the Dulmage–Mendelsohn, Kotzig–Lovász, and Gallai–Edmonds decompositions, are limited because they are only applicable to particular classes of graphs, such as bipartite graphs, or they are too sparse to provide sufficient information. To overcome these limitations, we introduce a new canonical decomposition that is applicable to all graphs and provides much finer information. This decomposition also provides the answer to the longstanding absence of a canonical decomposition that is nontrivially applicable to general graphs with perfect matchings. We focus on the notion of factor‐components as the fundamental building blocks of a graph; through the factor‐components, our new canonical decomposition states how a graph is organized and how it contains all the maximum matchings. The main results that constitute our new theory are the following: (i) a canonical partial order over the set of factor‐components, which describes how a graph is constructed from its factor‐components; (ii) a generalization of the Kotzig–Lovász decomposition, which shows the inner structure of each factor‐component in the context of the entire graph; and (iii) a canonically described interrelationship between (i) and (ii), which integrates these two results into a unified theory of a canonical decomposition. These results are obtained in a self‐contained way, and our proof of the generalized Kotzig–Lovász decomposition contains a shortened and self‐contained proof of the classical counterpart.
Screening Proteins That Interact With AcHog1 and the Functional Analysis of AcSko1 in Aspergillus cristatus
Authors: Shao Lei , Liu Zuoyi , Liu Yongxiang , Tan Yumei 
Published: October 2024

ABSTRACTAspergillus cristatus is a dominant fungus formed during the “flowering” process of Fuzhuan brick tea. Previous research has established that the sporulation of Aspergillus nidulans, a model organism of filamentous fungi, is regulated by light. However, the sporulation of A. cristatus is dependent on osmotic stress. In a previous study, we used pull‐down and mass spectrometry to identify proteins that interacted with AcHog1 in A. cristatus when cultured under different conditions of osmotic stress. In the present study, we analyzed the proteins we identified previously to investigate their functional role. The AA1E3BER4 protein was located downstream of Hog1 in the HOG branch pathway and was identified that was regulated by AcHog1. Furthermore, yeast two‐hybrid analysis showed that AA1E3BER4 interacted with AcHog1. In addition, we knocked out and complemented the Acsko1 gene encoding the AA1E3BER4 protein. We found that the number of sexual and asexual spores were downregulated by 3.81‐ and 4.57‐fold, respectively, in the ΔAcsko1 strain. The sensitivity of the ΔAcsko1 strain to sorbitol and sucrose, as regulators of osmotic stress, increased, and the sensitivity to high sucrose was higher than that of sorbitol. Acsko1 also regulated the response of A. cristatus to oxidative stress, Congo red, and SDS (sodium dodecyl sulfate). In addition, the deletion of Acsko1 significantly increased the pigment of the ΔAcsko1 strain. This is the first study to report the role of the sko1 gene in oxidative stress, stress‐induced damage to the cell wall, and pigment in Aspergillus cristatus.
Evaluation and projection of changes in temperature and precipitation over Northwest China based on CMIP6 models
Authors: Song Xuanyu , Xu Min , Kang Shichang , Wang Rongjun , Wu Hao 
Published: October 2024

AbstractNorthwest China is much more sensitive to climate warming, and the climate has varied rapidly from warm and drought to warm and humid conditions. In addition, due to the complex terrain of Northwest China, the methods and parameterization schemes of different CMIP6 models, these models are mostly applied to arid areas in Northwest China or Central Asia, lacking climate data for plateau areas and eastern Lanzhou, specifically in filtering CMIP6 models and evaluating applicable models. In this paper, 34 CMIP6 climate models are used to evaluate and forecast future trends in Northwest China under the SSP126, SSP245 and SSP585 scenarios in the short, medium and long term. CMIP6 models of temperature and precipitation are identified by applying the interannual variability skill score (IVS) between CN05.1 datasets and historical CMIP6 models, which are suitable for Northwest China. Then, we assess the characteristics, warming and wetting deviations, and uncertainties in the prediction of climatic change according to CMIP6 models over Northwest China. The results show that CMIP6 models in precipitation and temperature applicable to Northwest China are AWI‐CM‐1‐1‐MR, BCC‐CSM2‐MR, FGOALS‐g3, INM‐CM4‐8, INM‐CM5‐0 and MRI‐ESM2‐0. The multi‐model ensemble mean (MMEM) has better capability than individual CMIP6 models in precipitation and temperature prediction. Spatiotemporal climatic change over Northwest China shows overall warming and wetting trends. The IVS provides the ability to estimate CMIP6 model simulation performance both temporally and spatially. The temperature simulation is quite good in the Tarim Basin and Hexi Corridor region, and the precipitation simulation is quite good in the plateau region, Altai Mountains, Tianshan Mountains and Hexi Corridor region. Cold and wet deviations occur in Northwest China due to the topography and few stations, which are common reasons. The main sources of uncertainties in temperature prediction during this century are model uncertainty (before the 2090s) and scenario variability (after the 2090s), and model uncertainty in precipitation for CMIP6 becomes the main source of uncertainty.
Typology and impact of follower responses to social media influencer “promotional crisis”
Authors: Rynarzewska Ania Izabela , Nafees Lubna , Nikolov Atanas Nik 
Published: October 2024

AbstractThis study introduces a typology of follower responses to seemingly deceptive promotional content by a social media influencer (SMI) and the effects of those responses on observers. Through a large‐scale, mixed‐methods analysis of an influencer's “promotional crisis” in early 2023, popularly known as “Mascaragate” on TikTok, we identified follower engagement types that potentially harm both SMI and the promoted brand. The crisis sparked an unusually high level of network activity, providing valuable insights into the ripple effects of follower reactions. Drawing on parasocial relationship theory and social capital theory, we proposed that interactions between influencers and their followers, as well as among followers, evolve systematically through linguistic and social cues. These interactions can shift the original meaning of the influencer's message, leading to unintended consequences for both SMI and the brand. Through netnography and k‐means cluster analysis, we identified three distinct clusters of followers with unique attitudes toward the post and SMI. One group (i.e., “Type 1”) was particularly adept at reshaping the meaning of the SMI post through rational engagement with other followers. A subsequent experiment assessed the impact of each group's comments on the observers within a broader community network, focusing on credibility, skepticism, and the impact of social media dependency. The findings offer practical tools for brands and SMIs to analyze follower comment patterns and suggest strategies for addressing crises stemming from SMI‐linked promotional content, ultimately helping to preserve brand reputation and trust.
Variability of the Microbiota in Chronic Rhinosinusitis: A Scoping Review
Authors: Ccami‐Bernal Fabricio , Barriga‐Chambi Fernanda , Ortiz‐Benique Zhamanda N. , Ferrary Evelyne , Torres Renato 
Published: October 2024

AbstractObjectiveChronic rhinosinusitis (CRS) is characterized by a persistent inflammation of the nasal and paranasal sinus mucosa that could be potentially linked to a dysregulation between the microbiota and the immune system. We aim to explore general, methodological, and microbiological aspects of microbiota research in CRS compared to disease‐free individuals.Data SourcesEmbase, Ovid MEDLINE, PubMed, Scopus, and Web of Science.Review MethodsAll studies comparing the composition of the resident microbiota of the sinonasal cavities in 2 groups: CRS and normal participants. We conducted systematic study selection, data extraction, and analysis first using the title and abstract, and then the full texts based on predefined inclusion and exclusion criteria. Compiled and presented findings include sampling site and technique, and microbiological results such as the relative abundance and the variability of the composition of the microbiota in both groups.ResultsTwenty‐seven studies, using genomic identification with 16s RNA were analyzed. Case definitions primarily followed EPOS or AAO‐HNS guidelines, with endoscopic swabs (82%), and middle meatus sampling (74%) being prevalent techniques. Despite relative abundance variability, patterns emerged across studies, indicating an increase in Haemophilus (19%) and Pseudomonas (11%), and decrease in Propionibacterium (15%) and Anaerococcus (11%). Another pattern was observed, showing a decreased alpha diversity (6/19; 22%) in CRS compared to normal individuals.ConclusionWhile variations exist among studies, analysis of CRS microbiota suggests an association with dysbiosis, potentially contributing to chronic inflammation. Future research must prioritize standardized criteria for diagnostics and patient selection, fostering a more comprehensive understanding of CRS microbiota.
Outcomes in Minor Salivary Gland Tumors—A 20+ Year Tertiary‐Care Center Experience
Authors: Zhang Emily S. , Doty Samuel , Woody Neil , Bottalico Danielle , Ku Jamie A. , Prendes Brandon , Koyfman Shlomo A. , Campbell Shauna R. , Silver Natalie , Scharpf Joseph , Lamarre Eric D. 
Published: October 2024

AbstractObjectiveMinor salivary gland carcinomas are challenging to study due to their rarity and heterogeneity. We aim to further characterize clinical characteristics, treatment, and outcomes over 20 years within a single institution.Study DesignRetrospective chart review was conducted on 210 patients who received primary treatment for minor salivary gland malignancy from 2000 to 2022.SettingSingle tertiary‐care center.MethodsMultivariable Cox proportional hazards method was used to examine the relationship between pre‐determined clinically important variables and outcomes.ResultsFive‐year overall survival was 77.8% (72.0‐84.1). Advanced clinical T stage portended over a 2 times higher risk of death and recurrence. High pathologic grade was associated with a near 3 times higher risk of death and recurrence. There was a predominance of occult nodal metastases in level II for oral cavity and oropharynx site tumors.ConclusionClinical T stage and grade were important for overall survival, local, regional, and distant recurrence‐free survival. Occult nodal metastases occurred most often in level II.