bluuebunny/crossref_metadata_2025_split huggingface dataset:
D-aspartate stimulates growth hormone secretion in wethers
Authors: Takahashi Tatsuyuki , Kidachi Kyosuke , Yukawa Mikiko , Hachinohe Tomoki , Takashima Yuina , Fujimura Mao , Saito Atsuko , Soga Daichi , Ota Chihiro , Niizuma Eri , Sato Katsuyoshi , Ogasawara Hideki , Kurose Yohei 
Published: October 2024

Abstract
Growth hormone (GH) is an essential factor in enhancing the productivity of animals. In ruminants, L-aspartate (L-Asp) stimulates the secretion of GH; however, the effect of D-Asp on GH remains unknown. Here, we examined the effect of D-Asp on GH secretion in wethers. Blood GH, insulin, adrenaline, noradrenaline, non-esterified fatty acid (NEFA), and glucose concentrations were evaluated in response to the intravenous infusion of a high-dose (0.1 mmol/kg/min) of D-Asp for 20 min. Further, concentrations of these biomolecules were evaluated when a low-dose (0.05 mmol/kg/min) of D-Asp was continuously infused intravenously for 20 min. Finally, the direct effect of D-Asp on GH secretion was determined using cultured sections of the anterior pituitary tissue from wethers. Infusion of the high-dose of D-Asp markedly increased blood GH concentrations (P &lt; 0.05), resulting in an increase in the area under the curve (AUC). Plasma GH concentrations and AUC also increased in response to infusion of a low D-Asp dose. Infusion of a high and low D-Asp dose caused a prolonged reduction in plasma insulin concentrations, and the AUC was lower (P &lt; 0.05). Plasma NEFA concentrations gradually increased after the end of D-Asp infusion, with a low D-Asp dose infusion resulting in significantly higher concentrations at 90 min (P &lt; 0.05). Plasma adrenaline, noradrenaline, and glucose concentrations did not show significant changes despite differences in the dose of D-Asp. Although D-Asp treatments stimulated GH secretion in the cultured sections of pituitary tissues, the effect was not significant. These results suggest that D-Asp stimulates the secretion of GH in wethers through not only a direct action on the pituitary gland but also through another pathway of GH stimulation.
Dietary glycerides of valerate ameliorate diarrhea and impact intestinal physiology and serum biomarkers in weaned piglets infected with enterotoxigenic Escherichia coli F18
Authors: Kovanda Lauren , Rengman Sofia , Tawde Snehal , Pos Jeroen , Park Sangwoo , Sun Shuhan , Park Jungjae , Kim Kwangwook , Li Xunde , Liu Yanhong 
Published: October 2024

Abstract
In the commercial swine farm setting, the postweaning period is a critical window during which piglets are highly susceptible to infection and enterotoxigenic E. coli (ETEC)-associated diarrhea. Short-chain fatty acids and their glycerides are compounds that may influence intestinal health; however, valerate is one that has not been well-characterized for its role as a dietary supplement. Therefore, the major objective of this experiment was to investigate two forms of valerate glycerides on diarrhea, intestinal physiology, and systemic immunity of weaned pigs experimentally infected with ETEC F18. Dietary treatments included a control diet and three additional diets supplemented with 0.075% monovalerin, 0.1% monovalerin, or 0.1% trivalerin, respectively. Piglets were weaned (21 d to 24 d of age), individually housed, and experimental diets were fed through the 28-d trial period. After a 7-d period, all piglets were inoculated on three consecutive days with 1010 CFU ETEC F18/3 mL. Growth performance was monitored throughout the trial, and daily diarrhea scores were recorded. Rectal swabs were collected for bacterial culture to confirm the presence or absence of β-hemolytic coliforms throughout the trial. Serum samples were collected and analyzed for inflammatory biomarkers on days 0, 3, 6, and 21 postinoculation (PI) and untargeted metabolomics on day 6 PI. Intestinal mucosa and tissue sections were harvested from pigs sacrificed on day 7 PI for gene expression and histology analysis. All data, except for frequency of diarrhea and metabolomics, were analyzed by ANOVA using the PROC MIXED of SAS. Dietary trivalerin reduced (P &lt; 0.05) the frequency of severe diarrhea over the entire trial period and the frequency of β-hemolytic coliforms on day 7 PI compared with the control. The intestinal villus height on day 7 PI in jejunum tissue was increased (P &lt; 0.05) in pigs fed trivalerin. The mRNA expression of TNF-α was decreased (P &lt; 0.05) in the trivalerin group, while that of ZO1 was increased (P &lt; 0.05) compared with control. Throughout the trial, serum TNF-α was reduced in pigs fed trivalerin compared with control. Serum metabolites, adenosine, inosine, and shikimic acid were reduced (P &lt; 0.05) on day 6 PI in all treatment groups compared with control. In conclusion, the present results indicate supplementing dietary valerate glycerides exhibited beneficial impacts on diarrhea, inflammation, and intestinal gene expression of piglets during the postweaning period.
Parity-specific differences in spatial genetics and dispersal in the common lizard
Authors: Hunter Darren C , Clobert Jean , Elmer Kathryn R 
Published: October 2024

Abstract
Dispersal is a key demographic parameter that plays an important role in determining spatial population dynamics and genetic structure. Linking differences in dispersal patterns to life-history traits is often confounded by inconsistent environmental pressures experienced by different populations. To explore the relationship between dispersal and life history, we focus on a site where oviparous and viviparous lineages of the common lizard (Zootoca vivipara) are found adjacent to each other. We take advantage of this shared environment to investigate parity-specific dispersal patterns using high-resolution, individual-level spatial-genetic autocorrelation and population genomic approaches (11,726 single nucleotide polymorphisms; 293 oviparous and 310 viviparous individuals). We found isolation-by-distance patterns to be present in both the oviparous and viviparous populations. Density was 2.5 times higher in the oviparous population than the viviparous one, though heterozygosity and genetic diversity measures were similar in the two populations. We found marked differences in the extent of genetic neighbourhoods between the lineages, with the viviparous population showing both dispersal (σ) and spatial-genetic autocorrelation (Moran’s I) at 2-fold greater geographic distances than the oviparous population. We found clear evidence of male-biased dispersal from genetic estimates in the viviparous population. In the oviparous population, evidence of male-biased dispersal was weak or absent. These differences are likely to be closely linked to specific requirements of the alternative reproductive strategies and may be the demographic consequences of mother–offspring interactions. Fine-scale geographic and individual-level measures are essential to understanding parity mode differences at microevolutionary scales and to better identifying their ecological and evolutionary impacts.
Single-cell transcriptome analysis reveals atypical monocytes circulating ahead of acute graft-versus-host disease clinical onset
Authors: Martins Filipe , Planet Evarist , Marino Denis , Ansari Marc , Trono Didier 
Published: October 2024

Abstract
Acute graft-versus-host disease (aGVHD) represents the rejection of the recipient's skin, gut, and liver tissues of an allogeneic hematopoietic stem cell transplantation (HSCT) by the donor T cells. The onset of aGVHD is often rapid and its evolution is unpredictable. We undertook the single-cell RNA sequencing of peripheral blood mononuclear cells collected before aGVHD clinical onset in 3 patients and from 1 patient afterward. We used 4 HSCT recipients who remained free of aGVHD as controls. This analysis unveiled the presence of particular subpopulations of circulating monocytes and cytotoxic T cells (CTLs) in pre-aGVHD samples up to 18 d before clinical disease. These pre-aGVHD monocytes were characterized by an upregulation of the M2 polarity marker CD163 and the transmembrane protein SIGLEC1/CD169. At the same time, their CTL counterparts stood out for the upregulation of the CXCL10 receptor CXCR3 and the antigenic stimulation marker CD70. The occurrence of CD163/SIGLEC1 co-expressing monocytes upstream of aGVHD onset was validated using transcriptomic data from an independent cohort and by flow cytometry in additional blood samples. These findings point to potential early diagnostic tools and preventive therapeutic strategies for aGVHD.
The Role of Hospice and Palliative Medicine in the Ars Moriendi
Authors: Durham Levi 
Published: October 2024

Abstract
There is disagreement among physicians and medical ethicists on the precise goals of Hospice and Palliative Medicine (HPM). Some think that HPM’s goals should differ from those of other branches of medicine and aim primarily at lessening pain, discomfort, and confusion, while others think that HPM’s practices should aim, like all other branches of medicine, at promoting health. I take the latter position: using the ars moriendi to set a standard for what it means to die well, I argue that if HPM’s practices were to aim at mitigating suffering with little regard to promoting health, some patients would die worse deaths than if HPM’s practices were to aim at health. According to the ars moriendi, flourishing at the end of one’s life requires that persons exercise their agency and pursue the goods most important to them. On this view, HPM’s practices should promote patients’ health to enable them to pursue these goods.
On the role of surrogates in the efficient estimation of treatment effects with limited outcome data
Authors: Kallus Nathan , Mao Xiaojie 
Published: October 2024

Abstract
In many experimental and observational studies, the outcome of interest is often difficult or expensive to observe, reducing effective sample sizes for estimating average treatment effects (ATEs) even when identifiable. We study how incorporating data on units for which only surrogate outcomes not of primary interest are observed can increase the precision of ATE estimation. We refrain from imposing stringent surrogacy conditions, which permit surrogates as perfect replacements for the target outcome. Instead, we supplement the available, albeit limited, observations of the target outcome with abundant observations of surrogate outcomes, without any assumptions beyond unconfounded treatment assignment and missingness and corresponding overlap conditions. To quantify the potential gains, we derive the difference in efficiency bounds on ATE estimation with and without surrogates, both when an overwhelming or comparable number of units have missing outcomes. We develop robust ATE estimation and inference methods that realize these efficiency gains. We empirically demonstrate the gains by studying long-term-earning effects of job training.
Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients
Authors: Zhou Ping , Mo Dan , Huang Hanji , Xu Jiaqi , Liao Baoying , Wang Yinxue , Mao Di , Zeng Zhonghong , Huang Ziying , Zhang Chao , Yang Yihua , Yu Yang , Pan Heng , Li Rong 
Published: October 2024

Abstract
Recurrent implantation failure (RIF) is a leading impediment to assisted reproductive technology, yet the underlying pathogenesis of RIF remains elusive. Recent studies have sought to uncover novel biomarkers and etiological factors of RIF by profiling transcriptomes of endometrial samples. Nonetheless, the inherent heterogeneity among published studies and a scarcity of experimental validations hinder the identification of robust markers of RIF. Hence, we integrated six publicly accessible datasets with 209 samples, including microarray profiles of endometrial samples in the secretory phase. After removing batch effects, we identified 175 differentially expressed genes. Gene set enrichment analysis identified dysregulation of immunological pathways in RIF. We also observed altered immune infiltration and pro-inflammatory cytokines in RIF. Protein–protein interaction network analysis identified ten hub genes, representing two co-expression modules significantly related to RIF. Knockdown of ENTPD3, one of the hub genes, promoted the epithelial-mesenchymal transition process and resulted in elevated levels of pro-inflammatory cytokines. Collectively, our study reveals abnormal gene expressions involving the regulation of epithelial-mesenchymal transition and immune status in RIF, providing valuable insights into its pathogenesis.
Freedom, Omniscience and the Contingent A Priori
Authors: Lampert Fabio 
Published: October 2024

Abstract
One of the major challenges in the philosophy of religion is theological fatalism — roughly, the claim that divine omniscience is incompatible with free will. In this article, I present new reasons to be sceptical of what I consider to be the strongest argument for theological fatalism. First, I argue that divine foreknowledge is not necessary for an argument against free will if we take into account divine knowledge of contingent a priori truths. Second, I show that this argument can be generalized so that ordinary human knowledge of contingent a priori truths also leads to an argument against free will. This, I believe, results in an absurd conclusion that is unacceptable to both theists and non-theists. But if there is something wrong with this argument, there is something wrong, too, with the argument for theological fatalism. Although there is a range of possible responses, I suggest that the core issue in all cases is a closure principle — specifically, the principle that ‘no choice about’ is closed under entailment (or strict implication).
On the origin of star formation quenching in massive galaxies at z ≳ 3 in the cosmological simulations IllustrisTNG
Authors: Kurinchi-Vendhan Shalini , Farcy Marion , Hirschmann Michaela , Valentino Francesco 
Published: October 2024

ABSTRACT
Using the cosmological simulations IllustrisTNG, we perform a comprehensive analysis of quiescent, massive galaxies at z ≳ 3. The goal is to understand what suppresses their star formation so early in cosmic time, and how other similar mass galaxies remain highly star forming. As a first-order result, the simulations are able to produce massive, quiescent galaxies in this high-redshift regime. We find that active galactic nucleus (AGN) feedback is the primary cause of halting star formation in early, massive galaxies. Not only do the central, supermassive black holes (SMBHs) of the quenched galaxies have earlier seed times, but they also grow faster than in star-forming galaxies. As a result, the quenched galaxies are exposed to AGN feedback for longer, and experience the kinetic, jet mode of the AGN feedback earlier than the star-forming galaxies. The release of kinetic energy reduces inflows of gas while likely maintaining outflows, which keeps a low cold gas fraction and decreases the star formation of the galaxies down to a state of quiescence. In addition to AGN feedback, we also investigate the influence of the large-scale environment. While mergers do not play a significant role in the quenching process, the quenched galaxies tend to reside in more massive haloes and denser regions during their evolution. As this provides a greater initial amount of infalling gas to the galaxies, the large-scale environment can mildly affect the fate of the central SMBH growth and, via AGN feedback, contribute to star formation quenching.
Influence of rotation speed and frequency on the decision of Columba livia domestica (homing pigeon) to cross the rotor-swept area of paper blades mimicking a wind turbine
Authors: Blary Constance , Potier Simon , Duriez Olivier , Besnard Aurélien , Bonadonna Francesco 
Published: October 2024

ABSTRACT
To reduce bird collisions with wind turbines, automatic detection systems have been developed to slow the blades down when a bird is approaching. We experimentally tested whether blade rotational speed (i.e., number of rotations per min) and frequency (i.e., number of times a blade passes a point per min) affected the decision time (i.e., time to take-off), path choice (i.e., the position in the aviary), and decision to cross the rotor-swept area in Columba livia domestica (rock dove [domestic variety]; aka homing pigeon; hereafter, pigeon). We used a homemade device with paper blades, mimicking the movement of wind turbine blades. We adjusted the paper blade dimensions and achromatic contrast with the background to match the visual capabilities of pigeons, increasing the probability of detection. Pigeons were less likely to cross the rotor-swept area at higher speeds and frequencies, independent of their decision time. When pigeons crossed the rotor-swept area (43 out of 160 trials), 63% collided with the blades, regardless of blade speed or frequency. Pigeons chose to avoid the rotor-swept area after they had traveled half the distance to the wind turbine. Pigeons were not better able to avoid the rotor-swept area when blades were rotating at low speed and/or frequency and often collided with the blades. Thus, slowing blades to a low rotational speed may not reduce collisions with some species and a complete turbine shutdown may be necessary. The feasibility and economic costs of regular complete shutdowns after the deceleration triggered by the automatic detection systems need further investigation.
The H2S-responsive transcription factor ERF.D3 regulates tomato abscisic acid metabolism, leaf senescence, and fruit ripening
Authors: Hu Kangdi , Geng Meihui , Ma Lin , Yao Gaifang , Zhang Min , Zhang Hua 
Published: October 2024

Abstract
Hydrogen sulfide (H2S) is a signaling molecule that regulates plant senescence. In this study, we found that H2S delays dark-induced senescence in tomato (Solanum lycopersicum) leaves. Transcriptome and reverse transcription quantitative PCR (RT-qPCR) analyses revealed an ethylene response factor ERF.D3 is quickly induced by H2S. H2S also persulfidated ERF.D3 at amino acid residues C115 and C118. CRISPR/Cas9-mediated gene editing, and gene overexpression analyses showed that ERF.D3 negatively regulates leaf senescence and fruit ripening. Abscisic acid (ABA) levels were reduced by ERF.D3 overexpression, suggesting ERF.D3 might regulate ABA metabolism. Additionally, the ABA 8′-hydroxylase-encoding gene CYP707A2, which is required for ABA degradation, was identified as an ERF.D3 target gene through transcriptome data, RT-qPCR, dual-luciferase reporter assays, and electrophoretic mobility shift assays. ERF.D3 persulfidation enhanced its transcriptional activity toward CYP707A2. Moreover, the E3 ligase RNF217 ubiquitinated ERF.D3, which may accelerate fruit ripening during the late stage of fruit development. Overall, our study provides valuable insights into the roles of a H2S-responsive ERF.D3 and its persulfidation state in delaying leaf senescence and fruit ripening and provides a link between H2S and ABA degradation.
On the Within-Perfect Numbers
Authors: Kwan Chung-Hang , Miller Steven J 
Published: October 2024

Abstract
Motivated by the works of Erdös, Wirsing, Pomerance, Wolke and Harman on the sum-of-divisor function $\sigma(n)$, we study the distribution of a special class of natural numbers closely related to (multiply) perfect numbers which we term ‘$(\ell;k)$-within-perfect numbers’, where $\ell \gt 1$ is a real number and $k: [1, \infty) \rightarrow (0, \infty)$ is an increasing and unbounded function.
Absence of dysregulation in amplitude and phase of circadian rhythm of core body temperature in idiopathic hypersomnia: a case–control study
Authors: Adam Tugdual , Barateau Lucie , Tanty Jérôme , Dauvilliers Yves 
Published: October 2024

Abstract

Study Objectives
To investigate the amplitude and phase of the circadian rhythm of core body temperature (CBT) via the continuous measure of the gastrointestinal temperature in participants with idiopathic hypersomnia (IH), non-specified hypersomnia (NSH) compared to healthy controls (HC) in a constant routine standardized bedrest (BR) protocol.

Methods
Consecutive participants evaluated in a National Reference Center for Rare Hypersomnias benefited from an extensive evaluation with one-night polysomnography, followed by a modified Multiple Sleep Latency Test (mMSLT), and a continuous 32-hour BR recording in standardized conditions. CBT was recorded via a telemetry pill (e-Celsius) during the BR, modeled by a Cosinor, with extraction of MESOR, amplitude, and phase. Participants with IH, diagnosed according to the International Classification of Sleep Disorders-3, were compared with participants with NSH (complaint of hypersomnolence but normal mMSLT and BR), and HC. Participants were divided into four groups based on their mMSLT mean sleep latency (mMSLT+,≤8 minutes) and their BR total sleep time (BR+, ≥19 hours).

Results
A total of 108 participants (80% women, 28.3 ± 7.8 years old) were included in the analyses, 81 IH (83% women), 16 NSH (75% women), and 11 HC (64% women). Cosinor amplitude and phase of CBT did not differ between IH, NSH, and HC, nor in the subgroup analysis (37 BR+/mMSLT+, 35 BR+/mMSLT−, 9 BR-/mMSLT+, 27 BR-/mMSLT−). No difference in chronotypes was observed between groups. Women had a greater MESOR and reduced CBT amplitude compared to men.

Conclusions
The circadian rhythm of CBT showed no difference in amplitude or phase between IH, NSH, and HC, and was not related to prolonged sleep time or objective daytime sleepiness.
Phylogeny and biogeography support ancient vicariance and subsequent dispersal out of Africa in Palpimanidae spiders (Araneae)
Authors: Wood Hannah M , Kulkarni Siddharth , Ramírez Martín J , Scharff Nikolaj 
Published: October 2024

Abstract
The Palpimanidae are one of five extant Palpimanoidea families that occur mainly in South America and Africa, although there are lineages in Madagascar, islands of the Indian Ocean, and parts of Asia. Here we examine the role of plate tectonics in shaping the distribution of Palpimanidae. We perform molecular sequencing via target enrichment, which makes use of fragmented DNA, because most specimens are stored in natural history museum collections and were not properly preserved for molecular sequencing. We perform phylogenetic analysis, divergence dating, and ancestral range reconstructions to assess whether continental vicariance shaped the evolution of Palpimanidae. We also examine evolution of eye loss via ancestral character reconstruction. We report the first Palpimanidae phylogeny based on genomic data that samples the majority of Chediminae genera. Results suggest that Palpimanidae originated in the Triassic, with diversification spanning the Jurassic to the Cretaceous. Vicarance played a role in early diversification, with later range expansion out of Africa. Based on morphology and phylogenetic analysis, Sceliraptor is synonymized with Sarascelis, Sceliscelis is synonymized with Scelidocteus, and the new genus and species Sitamacho tao gen. nov., sp. nov. is described, with three species transferred from Hybosida.