bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustained synchronized neuronal network activity in a human astrocyte co-culture system
Authors: Kuijlaars Jacobine , Oyelami Tutu , Diels Annick , Rohrbacher Jutta , Versweyveld Sofie , Meneghello Giulia , Tuefferd Marianne , Verstraelen Peter , Detrez Jan R. , Verschuuren Marlies , De Vos Winnok H. , Meert Theo , Peeters Pieter J. , Cik Miroslav , Nuydens Rony , Brône Bert , Verheyen An 
Published: November 2016

AbstractImpaired neuronal network function is a hallmark of neurodevelopmental and neurodegenerative disorders such as autism, schizophrenia, and Alzheimer’s disease and is typically studied using genetically modified cellular and animal models. Weak predictive capacity and poor translational value of these models urge for better human derived in vitro models. The implementation of human induced pluripotent stem cells (hiPSCs) allows studying pathologies in differentiated disease-relevant and patient-derived neuronal cells. However, the differentiation process and growth conditions of hiPSC-derived neurons are non-trivial. In order to study neuronal network formation and (mal)function in a fully humanized system, we have established an in vitro co-culture model of hiPSC-derived cortical neurons and human primary astrocytes that recapitulates neuronal network synchronization and connectivity within three to four weeks after final plating. Live cell calcium imaging, electrophysiology and high content image analyses revealed an increased maturation of network functionality and synchronicity over time for co-cultures compared to neuronal monocultures. The cells express GABAergic and glutamatergic markers and respond to inhibitors of both neurotransmitter pathways in a functional assay. The combination of this co-culture model with quantitative imaging of network morphofunction is amenable to high throughput screening for lead discovery and drug optimization for neurological diseases.
Electronic hybridisation implications for the damage-tolerance of thin film metallic glasses
Authors: Schnabel Volker , Jaya B. Nagamani , Köhler Mathias , Music Denis , Kirchlechner Christoph , Dehm Gerhard , Raabe Dierk , Schneider Jochen M. 
Published: November 2016

AbstractA paramount challenge in materials science is to design damage-tolerant glasses. Poisson’s ratio is commonly used as a criterion to gauge the brittle-ductile transition in glasses. However, our data, as well as results in the literature, are in conflict with the concept of Poisson’s ratio serving as a universal parameter for fracture energy. Here, we identify the electronic structure fingerprint associated with damage tolerance in thin film metallic glasses. Our correlative theoretical and experimental data reveal that the fraction of bonds stemming from hybridised states compared to the overall bonding can be associated with damage tolerance in thin film metallic glasses.
Insights into a novel nuclear function for Fascin in the regulation of the amino-acid transporter SLC3A2
Authors: Saad Amine , Bijian Krikor , Qiu Dinghong , da Silva Sabrina Daniela , Marques Maud , Chang Chia-Hao , Nassour Hassan , Ramotar Dindial , Damaraju Sambasivarao , Mackey John , Bismar Tarek , Witcher Michael , Alaoui-Jamali Moulay A. 
Published: November 2016

AbstractFascin 1 (FSCN1) is a cytoskeleton-associated protein recognized to function primarily in the regulation of cytoskeleton structure and formation of plasma membrane protrusions. Here we report a novel nuclear function for Fascin 1. Biochemical studies and genome wide localization using ChIP-seq identified phosphorylated Fascin 1 (pFascin) in complexes associated with transcription and that it co-localizes with histone H3 Lys4 trimethylation (H3K4me3) on chromatin. Gene expression profiling identified genes affected by Fascin 1 including SLC3A2, a gene encoding for a plasma membrane transporter that regulates intracellular amino acid levels. RbBP5, a subunit of the H3K4 histone methyltransferase (HMT) complex was found to interact with Fascin 1 supporting its role in H3K4me3 establishment at target genes. Moreover, we show that changes to SLC3A2 levels affect amino acid-mediated mTORC1 activation. These results reveal that Fascin 1 has a yet undiscovered nuclear function as an epigenetic modulator of genes essential for amino acid metabolism.
Ethylene glycol mediated synthesis of SnS quantum dots and their application towards degradation of eosin yellow and brilliant green dyes under solar irradiation
Authors: Chowdhury Arpita Paul , Shambharkar Baban H. , Ghugal Sachin G. , Umare Suresh S. , Shende Ashok G. 
Published: November 2016

SnS (tin sulfide) quantum dots (QDs) were synthesized by a chemical coprecipitation method using ethylene glycol as a solvent and capping agent and thiourea as a sulfur source at a temperature of 160 °C, 4 h.
Shadows and spirals in the protoplanetary disk HD 100453
Authors: Benisty M. , Stolker T. , Pohl A. , de Boer J. , Lesur G. , Dominik C. , Dullemond C. P. , Langlois M. , Min M. , Wagner K. , Henning T. , Juhasz A. , Pinilla P. , Facchini S. , Apai D. , van Boekel R. , Garufi A. , Ginski C. , Ménard F. , Pinte C. , Quanz S. P. , Zurlo A. , Boccaletti A. , Bonnefoy M. , Beuzit J. L. , Chauvin G. , Cudel M. , Desidera S. , Feldt M. , Fontanive C. , Gratton R. , Kasper M. , Lagrange A.-M. , LeCoroller H. , Mouillet D. , Mesa D. , Sissa E. , Vigan A. , Antichi J. , Buey T. , Fusco T. , Gisler D. , Llored M. , Magnard Y. , Moeller-Nilsson O. , Pragt J. , Roelfsema R. , Sauvage J.-F. , Wildi F. 
Published: November 2016

Context. Understanding the diversity of planets requires studying the morphology and physical conditions in the protoplanetary disks in which they form.
Aims. We aim to study the structure of the ~10 Myr old protoplanetary disk HD 100453, to detect features that can trace disk evolution and to understand the mechanisms that drive these features.
Methods. We observed HD 100453 in polarized scattered light with VLT/SPHERE at optical (0.6 μm, 0.8 μm) and near-infrared (1.2 μm) wavelengths, reaching an angular resolution of ~0.02′′, and an inner working angle of ~0.09′′.
Results. We spatially resolve the disk around HD 100453, and detect polarized scattered light up to ~0.42′′ (~48 au). We detect a cavity, a rim with azimuthal brightness variations at an inclination of ~38° with respect to our line of sight, two shadows and two symmetric spiral arms. The spiral arms originate near the location of the shadows, close to the semi major axis. We detect a faint feature in the SW that can be interpreted as the scattering surface of the bottom side of the disk, if the disk is tidally truncated by the M-dwarf companion currently seen at a projected distance of ~119 au. We construct a radiative transfer model that accounts for the main characteristics of the features with an inner and outer disk misaligned by ~72°. The azimuthal brightness variations along the rim are well reproduced with the scattering phase function of the model. While spirals can be triggered by the tidal interaction with the companion, the close proximity of the spirals to the shadows suggests that the shadows could also play a role. The change in stellar illumination along the rim induces an azimuthal variation of the scale height that can contribute to the brightness variations.
Conclusions. Dark regions in polarized images of transition disks are now detected in a handful of disks and often interpreted as shadows due to a misaligned inner disk. However, the origin of such a misalignment in HD 100453, and of the spirals, is still unclear, and might be due to a yet-undetected massive companion inside the cavity, and on an inclined orbit. Observations over a few years will allow us to measure the spiral pattern speed, and determine if the shadows are fixed or moving, which may constrain their origin.
Effects of strength and endurance exercise order on endocrine responses to concurrent training
Authors: Jones Thomas W. , Howatson Glyn , Russell Mark , French Duncan N. 
Published: November 2016

AbstractThe present study examined the effect of strength and endurance training order on the endocrine milieu associated with strength development and performance during concurrent training. A randomised, between‐groups design was employed with 30 recreationally resistance‐trained males completing one of four acute experimental training protocols; strength training (ST), strength followed by endurance training (ST‐END), endurance followed by strength training (END‐ST) or no training (CON). Blood samples were taken before each respective exercise protocol, immediately upon cessation of exercise, and 1 h post cessation of exercise. Blood samples were subsequently analysed for total testosterone, cortisol and lactate concentrations. Ability to maintain 80% 1RM during strength training was better in ST and ST‐END than END‐ST (both p < .05). Immediately following the respective exercise protocols all training interventions elicited significant increases in testosterone (p < .05). ST and END‐ST resulted in greater increases in cortisol than ST‐END (both p < .05). The testosterone:cortisol ratio was similar following the respective exercise protocols. Blood lactate concentrations post‐training were greater following END‐ST and ST than ST‐END (both p < .05). Conducting endurance exercise prior to strength training resulted in impaired strength training performance. Blood cortisol and lactate concentrations were greater when endurance training was conducted prior to strength training than vice versa. As such, it may be suggested that conducting endurance prior to strength training may result in acute unfavourable responses to strength training when strength training is conducted with high loads.
The Potential Role for a Nitrogen Compliance Policy in Mitigating Gulf Hypoxia
Authors: Ribaudo Marc , Key Nigel , Sneeringer Stacy 
Published: November 2016

AbstractWe evaluate whether expanding compliance to include nitrogen management could be an effective tool to reduce excess nitrogen applications in the Mississippi and Atchafalaya River Basin (MARB). Compliance requires farmers to meet some minimum standard of environmental protection on environmentally sensitive land as a condition for receiving federal farm program benefits. Using farm‐level data on “excess” nitrogen applications and program benefits, we estimate the level of compliance and the reduction in excess nitrogen applications to cropland in the MARB. Extending compliance provisions to nitrogen management could reduce excess applications up to 60% under ideal conditions.
Shedding of Hepatitis C Virus Into the Rectum of HIV-infected Men Who Have Sex With Men
Authors: Foster Andrew L , Gaisa Michael M , Hijdra Rosanne M , Turner Samuel S , Morey Tristan J , Jacobson Karen B , Fierer Daniel S 
Published: November 2016

Abstract

Background
For over a decade we have known of an epidemic of sexually transmitted hepatitis C virus (HCV) infection among human immunodeficiency virus (HIV)-infected men who have sex with men (MSM), but there still remains significant controversy over which bodily fluid(s) are responsible for HCV transmission in these men.

Methods
We enrolled HIV-infected MSM with recent and chronic HCV infection and quantified HCV from rectal fluid obtained by blind swab. We compared the rectal HCV viral load (VL) with paired blood HCV VL.

Results
We found rectal HCV shedding in 20 (47%) of 43 men, only one (2%) of whom had visible bleeding. Detection of rectal HCV shedding was associated with blood VL &gt; 5 log10 IU/mL (p = .01), and 85% with blood VL &gt; 5 log10 IU/mL had rectal shedding. The HCV VL of the rectal fluid ranged from 2.6 to 5.5 log10 IU/mL. Based on the median rectal fluid VL, the surface of an average human penis would be exposed to at least 2,300 IU of HCV for the duration of anal intercourse.

Conclusion
This study provides the first direct evidence to our knowledge that a sufficient quantity of HCV is shed into the rectum in HIV-infected men with HCV infection to directly infect an inserted penis or be passed indirectly through fomite-like transmission to the rectum of sex partner. We must develop an appropriate public health campaign to educate MSM about these routes of HCV infection to reverse the HCV epidemic among HIV-infected MSM.
Comparing the Outcomes of Patients With Carbapenemase-Producing and
Non-Carbapenemase-Producing Carbapenem-Resistant
Enterobacteriaceae Bacteremia
Authors: Tamma Pranita D. , Goodman Katherine E. , Harris Anthony D. , Tekle Tsigereda , Roberts Ava , Taiwo Abimbola , Simner Patricia J. 
Published: November 2016

Abstract

Background.
Carbapenem-resistant Enterobacteriaceae (CRE) are associated with considerable mortality. As mechanisms of carbapenem resistance are heterogeneous, it is unclear if mortality differs based on resistance mechanisms. We sought to determine whether CRE resistance mechanism determination is prognostically informative.

Methods.
We conducted an observational study comparing 14-day mortality between patients with carbapenemase-producing (CP)-CRE compared with non-CP-CRE bacteremia. Clinical data were collected on all patients. A comprehensive DNA microarray-based assay was performed on all isolates to identify β-lactamase-encoding genes.

Results.
There were 83 unique episodes of monomicrobial CRE bacteremia during the study period: 37 (45%) CP-CRE and 46 (55%) non-CP-CRE. The majority of CP-CRE isolates were blaKPC (92%), followed by blaNDM (5%) and blaOXA-48-type (3%). CP-CRE isolates were more likely to have meropenem minimum inhibitory concentrations (MICs) ≥16 µg/mL, while non-CP-CRE isolates were more likely to have meropenem MICs ≤1 µg/mL (P value &lt; .001). A total of 18 (22%) patients died within 14 days, including 12 (32%) in the CP-CRE group and 6 (13%) in the non-CP-CRE group. Adjusting for severity of illness on day 1 of bacteremia, underlying medical conditions, and differences in antibiotic treatment administered, the odds of dying within 14 days were more than 4 times greater for CP-CRE compared with non-CP-CRE bacteremic patients (adjusted odds ratio, 4.92; 95% confidence interval, 1.01–24.81).

Conclusion.
Our findings suggest that CP-CRE may be more virulent than non-CP-CRE and are associated with poorer outcomes. This underscores the added importance of delineating underlying resistance mechanisms of CRE to direct antibiotic treatment decisions.
Discovery of a supercluster in the Zone of Avoidance in Vela
Authors: Kraan-Korteweg Renée C. , Cluver Michelle E. , Bilicki Maciej , Jarrett Thomas H. , Colless Matthew , Elagali Ahmed , Böhringer Hans , Chon Gayoung 
Published: November 2016

Abstract
We report the discovery of a potentially major supercluster that extends across the Galactic plane in the constellation of Vela, at a mean recessional velocity of ∼18 000 km s−1. Recent multiobject spectroscopic observations of this Vela supercluster (VSCL), using AAOmega+2dF and the Southern African Large Telescope, confirm an extended galaxy overdensity in the Zone of Avoidance (ZOA) located where residual bulk flows predict a considerable mass excess. We present a preliminary analysis of ∼4500 new spectroscopic galaxy redshifts obtained in the ZOA centred on the Vela region ($l = 272{^{\circ}_{.}}5 \pm 20^\circ , b = 0^\circ \pm 10^\circ$). The presently sparsely sampled data set traces an overdensity that covers $25^\circ$ in Galactic longitude on either side of the Galactic plane, suggesting an extent of 25 × 20 deg2, corresponding to ∼ 115 × 90 h70 Mpc at the supercluster redshift. In redshift space, the overdensity appears to consist of two merging wall-like structures, interspersed with clusters and groups. Both the velocity histogram and the morphology of the multibranching wall structure are consistent with a supercluster classification. $K_{\rm s}^{\rm o}$ galaxy counts show an enhancement of ∼1.2 over the survey area for galaxies brighter than $M_{K}^{\ast }$ at the VSCL distance, and a galaxy overdensity of δ = 0.50–0.77 within a photometric redshift shell around the VSCL, when compared with various Two Micron All-Sky Survey samples. Taking account of selection effects, the VSCL is estimated to contribute $v_\mathrm{LG} \gtrsim 50$ km s−1 to the motion of the Local Group.
Expansion of the substrate range of the gentisate 1,2-dioxygenase from Corynebacterium glutamicum for the conversion of monohydroxylated benzoates
Authors: Eppinger Erik , Stolz Andreas 
Published: November 2016

AbstractThe gentisate 1,2-dioxygenases (GDOs) from Corynebacterium glutamicum and various other organisms oxidatively cleave the aromatic nucleus of gentisate (2,5-dihydroxybenzoate), but are not able to convert salicylate (2-hydroxybenzoate). In contrast, the α-proteobacterium Pseudaminobacter salicylatoxidans synthesises an enzyme (‘salicylate dioxygenase’, SDO) which cleaves gentisate, but also (substituted) salicylate(s). Sequence comparisons showed that the SDO belongs to a group of GDOs mainly originating from Gram-positive bacteria which also include the GDO from C. glutamicum ATCC 13032. The combination of sequence comparisons with previously performed structural and mutational analyses of the SDO allowed to identify an amino acid residue (Ala112) which might prevent the oxidation of (substituted) salicylate(s) by the GDO from C. glutamicum. Therefore, the relevant mutation (Ala→Gly) was introduced into the GDO from C. glutamicum. The GDO variant obtained gained the ability to oxidise salicylate and several other monohydroxylated substrates. In order to screen a broader range of enzyme variants a chromogenic assay was developed which allowed the detection of bacterial colonies converting salicylate. The applicability of this test system was proven by screening a set of GDO variants obtained by saturation mutagenesis at different positions. This demonstrated that also GDO variants carrying the mutations Ala112→Ser, Ala112→Ile and Ala112→Asp converted salicylate.
The effect of a budget-based incentive compensation scheme on job performance
Authors: Chong Vincent K. , Law Maggie B.C. 
Published: November 2016

PurposeThis study aims to examine the role of trust-in-supervisor and organizational commitment on the relationship between a budget-based incentive compensation scheme and job performance.Design/methodology/approachA survey was conducted involving 120 managers from Australian manufacturing firms listed in the Who’s Who in Business in Australia electronic database. A partial least squares approach was used to assess the psychometric properties of the theoretical model and proposed hypotheses. Data analysis was conducted using WarpPLS Version 5.0.FindingsThe results suggest that the reliance on a high budget-based incentive compensation scheme was found to lead to higher trust-in supervisor, which in turn resulted in higher organizational commitment and improved subordinate job performance.Research limitations/implicationsThis study is subject to the limitations of survey-based research.Practical implicationsThis study may assist top management to better understand the importance of designing an effective budget-based incentive compensation scheme to promote high interpersonal trust and organizational commitment among subordinates. Cultivating a climate of trust may help to enhance interpersonal trust between subordinates and their superior, which in turn may lead to higher levels of organizational commitment and improvement in subordinate job performance.Originality/valueThis paper elucidates and contributes to the existing literature by suggesting that a budget-based incentive compensation scheme can directly affect subordinates’ level of trust in their supervisor, and that trust-in-supervisor can serve as an antecedent to the development and cultivation of subordinates’ commitment to the organization, which in turn improves their job performance.
Project control: an exploratory study of levers of control in the context of managing projects
Authors: Rezania Davar , Baker Ron , Burga Ruben 
Published: November 2016

PurposeThis paper aims to examine the levers of control (LOC) framework in the context of managing projects. The authors explore the impact of diagnostic systems, interactive systems, beliefs systems and boundary systems on project performance and explore the association between control levers.Design/methodology/approachUsing data gathered from 113 project managers representing 38 organizations, the authors used the partial least squares path modelling algorithm to analyse the data.FindingsThe analysis validates the LOC framework in the context of managing projects and reveals the interrelatedness of control systems and their impact on project performance.Research limitations/implicationsThe conclusions support the current emphasis on performance reporting and change control and highlight the need to consider the interdependencies between control levers.Originality/valueThis study re-conceptualizes project control by using the LOC framework in the context of managing projects as temporary organizations. This provides a model for investigating and understanding project management control systems that consider the interaction of control mechanisms. Furthermore, the associations between the four control systems and project performance are examined, rather than individual mechanisms in isolation.
Coastal tourism space in the context of climate change: discourses and strategies
Authors: Lapointe Dominic , Lebon Coralie , Guillemard Alexis 
Published: November 2016

Purpose
The purpose of the paper is to explore how climate change and the discourses about adaptation to climate change are altering the spatial development of the tourism industry in coastal destinations. The paper also identifies how tourist development and climate change adaptation can be combined to transform space and place, especially in coastal tourism areas.

Design/methodology/approach
Using a theoretical approach based on the concept of production of space, the study focuses on relational space, relationships expressed through representations of space and social practices. A case study method is used to analyze the socio-geographic processes at work in the adaptation to climate change in a coastal tourism community in Quebec, Canada. The analysis of the study utilized Nvivo with thematic textual queries.

Findings
The results reveal an adaptation process at work, based on a “hold the line” strategy, where private stakeholders choose to invest in defensive structures to mitigate the impact of rising sea levels and erosion. This strategy reflects coordinated action in the face of the risk and to protect high-value land property.

Research limitations/implications
This research illustrates how tourism and climate change adaptation discourses intersect. It also reveals how tourism development promotes the values and image of coastal space and how this can conflict with an efficient climate change adaptation strategy.

Originality/value
This research provides guidelines for coastal tourism communities, enabling them to design their own climate change adaptation strategy, taking into account how the different social discourses and tourism practices interact with climate change adaptation. It also provides some insights into the criteria that influence an effective climate change adaptation strategy.