bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guide to managing persistent upper gastrointestinal symptoms during and after treatment for cancer
Authors: Andreyev H Jervoise N , Muls Ann C , Shaw Clare , Jackson Richard R , Gee Caroline , Vyoral Susan , Davies Andrew R 
Published: October 2016

BackgroundGuidance:the practical management of the gastrointestinal symptoms of pelvic radiation diseasewas published in 2014 for a multidisciplinary audience. Following this, a companion guide to managing upper gastrointestinal (GI) consequences was developed.AimsThe development and peer review of an algorithm which could be accessible to all types of clinicians working with patients experiencing upper GI symptoms following cancer treatment.MethodsExperts who manage patients with upper GI symptoms were asked to review the guide, rating each section for agreement with the recommended measures and suggesting amendments if necessary. Specific comments were discussed and incorporated as appropriate, and this process was repeated for a second round of review.Results21 gastroenterologists, 11 upper GI surgeons, 9 specialist dietitians, 8 clinical nurse specialists, 5 clinical oncologists, 3 medical oncologists and 4 others participated in the review. Consensus (defined prospectively as 60% or more panellists selecting ‘strongly agree’ or ‘agree’) was reached for all of the original 31 sections in the guide, with a median of 90%. 85% of panellists agreed that the guide was acceptable for publication or acceptable with minor revisions. 56 of the original 61 panellists participated in round 2. 93% agreed it was acceptable for publication after the first revision. Further minor amendments were made in response to round 2.ConclusionsFeedback from the panel of experts developed the guide with improvement of occasional algorithmic steps, a more user-friendly layout, clearer time frames for referral to other teams and addition of procedures to the appendix.
A new posterior iliac puncture/aspiration needle
Authors: Islam Anwarul 
Published: October 2016

AimsThe needles that are currently used for obtaining bone marrow aspirate samples from the posterior ilium are typically those of 1930s vintage (eg, Klima, Salah or similar needles), which were specifically designed for sternal aspiration. These needles are not designed to obtain bone marrow aspirate samples from the posterior ilium and as a result they are unsatisfactory particularly if the patient is large or obese. A new posterior iliac puncture/aspiration needle has therefore been designed, which is particularly suited for bone marrow aspiration from the posterior ilium.MethodsThe needle was tested on five cadavers and on five patients.ResultsThe design and construction of the needle was found to be satisfactory and a marked improvement over the conventional sternal puncture needles particularly when large or obese patients were concerned.ConclusionsThe new posterior iliac bone marrow aspiration needle has advantages that overcome the limitations of using a conventional sternal puncture needle to obtain marrow aspirates from the posterior ilium.
The incidence of IgG4-positive plasma cells staining TIN in patients with biopsy-proven tubulointerstitial nephritis
Authors: Mac Kathy , Wu Xiao Juan , Mai Jun , Howlin Kenneth , Suranyi Michael , Yong Jim , Makris Angela 
Published: October 2016

AimIgG4 disease is rare. However, IgG4 tubulointerstitial nephritis (TIN) is the most common renal manifestation. IgG4 disease is usually associated with elevated serum IgG4 levels and other organ involvement, low-density renal lesions on enhanced CT imaging and immune activation. The incidence of IgG4-TIN may be underestimated, as staining for IgG4 is not routine. This study sought to describe the prevalence of previously undiagnosed IgG4-TIN. Due to the complexity of the diagnosis, we only attempt to look at IgG4-positive plasma cell TIN as a potential indication for IgG4 renal disease.MethodsA retrospective review of native renal biopsies performed between 2002 and 2012 with a primary diagnosis of TIN was selected. Samples for which interstitial nephritis was secondary to a glomerular disease were excluded. The tissues were stained for IgG4 and scored by two blinded observers. Demographic and follow-up details were collected. This study was approved by the local ethics committee.Results82 cases of interstitial nephritis from a total of 1238 renal biopsies (2002–2012) were available after staining for further assessment. 12 samples demonstrated staining consistent with the criteria for IgG4-positive plasma cell TIN, of which 3 had mildly positive staining, 7 moderately positive staining and 2 had markedly positive staining. There were no statistically significant differences in the baseline characteristics between the positive and negative staining groups.ConclusionsA number of cases of IgG4-positive plasma cell TIN were observed histologically that had been previously diagnosed as non-specific chronic TIN. IgG4-positive plasma cell TIN made up 1% of all renal biopsies performed over 10 years and 13% of all biopsies demonstrating TIN not related to glomerular disease. IgG4 staining should be considered routinely in biopsies demonstrating primary TIN.
Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch
Authors: Brea María S. , Díaz Romina G. , Escudero Daiana S. , Caldiz Claudia I. , Portiansky Enrique L. , Morgan Patricio E. , Pérez Néstor G. 
Published: October 2016

Background
Myocardial stretch increases force biphasically: the Frank‐Starling mechanism followed by the slow force response (SFR). Based on pharmacological strategies, we proposed that epidermal growth factor (EGF) receptor (EGFR or ErbB1) activation is crucial for SFR development. Pharmacological inhibitors could block ErbB4, a member of the ErbB family present in the adult heart. We aimed to specifically test the role of EGFR activation after stretch, with an interference RNA incorporated into a lentiviral vector (small hairpin RNA [shRNA]‐EGFR).

Methods and Results
Silencing capability of p‐shEGFR was assessed in EGFR‐GFP transiently transfected HEK293T cells. Four weeks after lentivirus injection into the left ventricular wall of Wistar rats, shRNA‐EGFR–injected hearts showed ≈60% reduction of EGFR protein expression compared with shRNA‐SCR–injected hearts. ErbB2 and ErbB4 expression did not change. The SFR to stretch evaluated in isolated papillary muscles was ≈130% of initial rapid phase in the shRNA‐SCR group, while it was blunted in shRNA‐EGFR–expressing muscles. Angiotensin II (Ang II)‐dependent Na+/H+ exchanger 1 activation was indirectly evaluated by intracellular pH measurements in bicarbonate‐free medium, demonstrating an increase in shRNA‐SCR–injected myocardium, an effect not observed in the silenced group. Ang II‐ or EGF‐triggered reactive oxygen species production was significantly reduced in shRNA‐EGFR–injected hearts compared with that in the shRNA‐SCR group. Chronic lentivirus treatment affected neither the myocardial basal redox state (thiobarbituric acid reactive substances) nor NADPH oxidase activity or expression. Finally, Ang II or EGF triggered a redox‐sensitive pathway, leading to p90RSK activation in shRNA‐SCR‐injected myocardium, an effect that was absent in the shRNA‐EGFR group.

Conclusions
Our results provide evidence that specific EGFR activation after myocardial stretch is a key factor in promoting the redox‐sensitive kinase activation pathway, leading to SFR development.
The Reproduction and Crisis of Capitalism in Venezuela under Chavismo
Authors: Dachevsky Fernando , Kornblihtt Juan 
Published: October 2016

The current crisis in Venezuela is sometimes said to have been provoked by the response of imperialism and the local oligarchy to the fundamental changes in economic and political relations fostered during the administrations of Hugo Chávez. A quantitative study using various statistical sources shows that the significant increase in oil rent during the Chávez presidency did not translate into a qualitative transformation in the form of state intervention and that, although social expenditures increased in that period, most of the income that allowed this was obtained through currency overvaluation by inefficient national and foreign capital. The current crisis is, therefore, evidence of the limits of low-productivity state and private capital reproduction due to the decline in oil prices rather than of a conflict between overcoming and reproducing capitalism in an alleged “economic war.” A veces se dice que la crisis actual en Venezuela ha sido provocada por la respuesta del imperialismo y la oligarquía local a los cambios profundos en las relaciones económicas y políticas promovidos durante la administración de Hugo Chávez. Un estudio cuantitativo en el que se usaron diferentes fuentes estadísticas demuestra que el aumento significativo en la renta del petróleo durante la presidencia de Chávez no se tradujo en una transformación cualitativa de la intervención estatal y que, aunque los gastos sociales aumentaron en ese período, la mayor parte del ingreso que permitió ese aumento se obtuvo por medio de la sobrevaluación de la moneda por parte del ineficiente capital nacional y extranjero. Por lo tanto, la crisis actual es evidencia de los límites de la baja productividad de la reproducción estatal y privada debido a la caída en el precio del petróleo y no un conflicto entre la eliminación y la reproducción del capitalismo en una supuesta “guerra económica.”
Trehalose preincubation increases mesenchymal (CD271<sup>+</sup>) stem cells post-cryopreservation viability
Authors: Kusuma Indra , Hadi Restu S. , Kiranadi Bambang , Boediono Arief 
Published: October 2016

Background: Dimethyl sulfoxide (Me2SO) is a common cryoprotective agent widely used in cell preservation system. Me2SO is currently known to cause epigenetic changes which are critical in stem cells development and cellular differentiation. Therefore, it is imperative to develop cryopreservation techniques that protect cellular functions and avert Me2SO adverse effect. Trehalose was able to protect organism in extreme condition such as dehydration and cold. This study aimed to verify the protective effect of trehalose preincubation procedure in cryopreservation.Methods: The study was conducted using experimental design. Thawed mesenchymal (CD271+) stem cells from YARSI biorepository were used for the experiment. Trehalose preincubation was performed for 1 hour, internalized trehalose was confirmed by FTIR-ATR measurement. Three groups consisted of (1) cryopreserved without trehalose preincubation, (2) cryopreserved with trehalose preincubation, and (3) did not undergo cryopreservation were evaluated after 24 hours in LN2 for viability in culture. The absorbance from each group was measured at 450 nm. The analysis performed using paired student t test.Results: Viability of thawed mesenchymal (CD271+) stem cells that undergo trehalose preincubation prior cryopreservation was significantly higher (p<0.05) compared to group without trehalose preincubation. Higher viability observed between group with trehalose preincubation compared with controlled group suggests protection to trypsinization. Mesenchymal (CD271+) stem cells incubated for 1 hour in 100 mM trehalose supplemented medium results in 15% trehalose loading efficiency.Conclusion: These findings confirm the protective effect of trehalose preincubation in cryopreservation. Future research should be directed to elucidate the trehalose internalization mechanism and eventually the protective mechanism of trehalose in mammalian cell cryopreservation.
Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage
Authors: Ekawati Maria , Mujihartini Ninik , Jusuf Ahmad A. , Dharmasetiawani Nani , Jusman Sri W.A. , Sadikin Mohamad 
Published: October 2016

Background: Placental hypoxia may lead to oxidative stress, which inflicts damage to capillary protein junction. The aim of this study was to evaluate altered expression of endothelial junction protein of capillaries in hypoxia condition and to observe its correlation with the incidence of intraventricular hemorrhage in premature infants.Methods: A cross-sectional study was conducted by using placental tissues of premature infants as amodel of capillary integrity (29 hypoxic and 29 non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione (GSH) served as markers of oxidative stress. The expressions of junctional proteins, N-cadherin and occludin were analyzed by immunohistochemistry. Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to analyze the data.Results: The HIF-1α and MDA levels were slightly, but not significantly, higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue (ranged 1.92–93.61), respectively} compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77 pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both groups (38.14 (ranged 9.44–118.91) and 38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia comparedto non-hypoxia group (p=0,001), while protein expression of N-cadherin (3.4; 75.9; 6.9; 13.8%) and occludin (20.7; 3.4; 69.0; 3.4; 6.9%) in hypoxia group was not associated with IVH (p=0.783 and p=0.743).Conclusion: Hypoxia altered expression of endothelial junction protein in placental capillaries, but no association with intraventricular hemorrhage was observed.
Antiproliferative activity and caspase enhancement properties of <em>Annona muricata</em> leaves extract against colorectal cancer cells
Authors: Indrawati Lili , Ascobat Purwantyastuti , Bela Budiman , Abdullah Murdani , Surono Ingrid S. , Pramono Suwijiyo 
Published: October 2016

Background: The prevalence of colorectal cancer is rising in Asia including Indonesia. Annona muricata tea leaves, that is traditionally used for maintaining health, and lately being used by cancer patients. The objectives of this study is to investigate its effects in human colorectal cancer cell in vitro and ex vivo.Methods: Thirty patients with colorectal cancer (CRC) were enrolled in a randomized double-blind placebo-controlled trial. They were equally divided into two groups: those treated with 300 mg A. muricata leaf extract and placebo daily for 8 weeks. Serum from supplemented CRC patients of both groups was compared for caspase 9 and caspase 8 enhancement activity. Antiproliferative effect of water extract of A. muricata leaves and its fractions were evaluated against colorectal cancer cell line (DLD-1 and COLO 205) compared with 5-fluorouracil and placebo, the dose range was 62.5-2,000 µg/mL. Method used was 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data were analyzed by Mann-Whitney U test. The p value was set at 0.05.Results: Ethanol-soluble fraction of A. muricata leaves extract water extract (ESFAM) leaves extract had cytotoxicity effects on DLD-1 as well as COLO 205 cell line, as shown by the lower IC50 compared to 5-fluorouracil and placebo, 20.59 μg/mL and 654.9μg/mL, respectively. Serum of subjects supplemented with extract significantly induced caspase 9 (p=0.001) activity of DLD-1 colorectal cancer cell line, but not for caspase 8 activity (p=0.372).Conclusion: The study's results suggest the cytotoxicity potential of A. muricata leaves extract in in vitro and ex vivo studies.
Risk factors of sepsis after open congenital cardiac surgery in infants: a pilot study
Authors: Fakhri Dicky , Busro Pribadi W. , Rahmat Budi , Purba Salomo , Mukti Aryo A.P. , Caesario Michael , Christy Kelly , Santoso Anwar , Djauzi Samsuridjal 
Published: October 2016

Background: Postsurgical sepsis is one of the main causes of the high mortality and morbidity after open congenital heart surgery in infants. This study aimed to evaluate the role of cardiopulmonary bypass duration, thymectomy, surgical complexity, and nutritional status on postsurgical sepsis after open congenital cardiac surgery in infants.Methods: A total of 40 patients <1 year of age with congenital heart disease, Aristotle Basic Score (ABS) ≥6 were followed for clinical and laboratory data before and after surgery until the occurrence of signs or symptoms of sepsis or until a maximum of 7 days after surgery. Bivariate analyses were performed. Variables with p≤0.200 were then included for logistic regression.Results: Duration of cardiopulmonary bypass ≥90 minutes was associated with 5.538 increased risk of postsurgical sepsis in comparison to those ≤90 minutes (80% vs 25%, RR=5.538, p=0.006). No association was observed between the incidence of postsurgical sepsis with poor nutritional status (86% vs 84%, RR=1.059, p=1.000), thymectomy (and 50% vs 76%, RR=0.481, p=0.157), and Aristotle Basic Score (p=0.870).Conclusion: Cardiopulmonary bypass time influences the incidence of sepsis infants undergoing open congenital cardiac surgery. Further studies are needed to elaborate a number of risk factors associated with the incidence of sepsis in this population.
Nursing Clinical Instructor Experiences of Empowerment in Rwanda: Applying Kanter’s and Spreitzer’s Theories
Authors: Thuss Mary , Babenko-Mould Yolanda , Andrusyszyn Mary-Anne , Laschinger Heather K. S. 
Published: October 2016

AbstractThe purpose of this study was to explore Rwandan nursing clinical instructors’ (CIs) experiences of structural and psychological empowerment. CIs play a vital role in students’ development by facilitating learning in health care practice environments. Quality nursing education hinges on the CI’s ability to enact a professional role. A descriptive qualitative method was used to obtain an understanding of CIs empowerment experiences in practice settings. Kanter’s Theory of Structural Power in Organizations and Spreitzer’s Psychological Empowerment Theory were used as theoretical frameworks to interpret experiences. Interview data from 21 CIs were used to complete a secondary analysis. Most participants perceived the structural components of informal power, resources, and support while formal power and opportunity were limited, diminishing their sense of structural empowerment. Psychological empowerment for CIs stemmed from a sense of competence, meaning, impact and self-determination they had for their teaching roles and responsibilities in the practice setting.
Examining the Importance of Admissions Criteria in Predicting Nursing Program Success
Authors: Wambuguh Oscar , Eckfield Monika , Van Hofwegen Lynn 
Published: October 2016

AbstractBaccalaureate nursing programs select students likely to graduate, become licensed, and contribute to a diverse workforce, and admissions criteria need to support those goals. This study assessed five criteria: pre-admit science GPA; TEAS score; healthcare experience; previous baccalaureate degree; and pre-admission university enrollment vs. college transfer as predictors of three desired outcomes: graduation; nursing program GPA; and passing NCLEX-RN. Results found TEAS and pre-admit science GPA predicted nursing program outcomes. Students with TEAS≥82 had 8 % greater probability of graduating, 13 % greater probability of a GPA≥3.25, and 9 % greater probability of passing NCLEX-RN, compared to students with TEAS < 82. Students with pre-admit science GPAs≥3.8 had 11 % greater probability of passing NCLEX-RN and 14 % greater probability of a GPA≥3.25 compared to students with pre-admit science GPAs < 3.8. Further discussions regarding factors important for training a diverse nursing workforce and effective ways to implement non-academic admission criteria are warranted.
Failing Clinical Practice & the Unsafe Student: A New Perspective
Authors: Scanlan Judith Marlene , Chernomas Wanda M. 
Published: October 2016

AbstractStudents who fail clinical courses is a long standing issue in nursing education. Although faculty intuitively “know” a student is in clinical difficulty, the research literature is limited to delineating and describing characteristics of these students. A retrospective analysis of students’ files in which there was at least one clinical failure was conducted to identify clinical failure indicators. Files included students who were successful, required to withdraw, or voluntarily withdrew. This study integrates these characteristics in a manner not discussed in the literature. Two themes emerged that characterize student practices: (i) How students are in practice and (ii) Aspects of practice. A third theme surfaced as clinical teachers responded to these students by labelling the practice unsafe and increasing vigilance. A model was developed that shows the relationship between these characteristics and unsafe student practice.
For Safety and Security Reasons: The Cost of Component-Isolation in IoT
Authors: Zuepke Alexander , Beckmann Kai , Zoor Andreas , Kroeger Reinhold 
Published: October 2016

Abstract
The current development trend of Internet of Things (IoT) aims for a tighter integration of mobile and stationary devices via various networks. This includes communication of vehicles to roadside infrastructure (V2I), as well as intelligent sensors / actors in Logistics and smart home environments.
Compared to isolated traditional embedded systems, the exposure to open networks increases the attack surface, and errors in the networking components could compromise the safety and security of the embedded application or the whole network. But often current system architectures for mass-market IoT devices lack the required isolation concepts.
Using a partitioning microkernel and enforcing the use of a microcontroller’s memory protection unit (MPU) facilities, we compare different isolation concepts for a publish/subscribe middleware implementing OMG’s Data Distribution Service (DDS) standard and we evaluate our results on an STM32F4 microcontroller. The results of this case study show moderate costs for increased memory usage and additional context switches.
Formation of Chromium-Containing Molten Salt Phase during Roasting of Chromite Ore with Sodium and Potassium Hydroxides
Authors: Escudero-Castejon Lidia , Sanchez-Segado Sergio , Parirenyatwa Stephen , Jha Animesh 
Published: October 2016

AbstractChromium has a wide range of applications including metals and alloys manufacturing, pigments, corrosion resistance coatings and leather tanning. The production of chromium chemicals is based on the oxidative alkali roasting of chromite ores, which leads to the formation of water-soluble alkali chromates. Previous investigations reported that when chromite is roasted with soda-ash, a molten salt containing chromium, which is mainly composed of sodium carbonate and sodium chromate (Na2CO3-Na2CrO4 binary mixture), forms under typical roasting conditions. The physical properties of the liquid phase, which are dependent on the temperature, charge and gangue compositions, play an important role on the oxidation reaction and may limit the chromate recovery by hindering the oxygen transport to the reaction interface. This investigation focuses on the alkali roasting of chromite ore at 1,000 oC using NaOH and KOH, followed by water leaching. The influence of the alkali ratio on the chromium extraction yield is analysed, and the results obtained with both hydroxides are compared. Sample characterisation and thermodynamic analysis, including phase diagrams, equilibrium calculations and computation of liquidus curves, are combined with the purpose of studying the formation of the molten salt phase under different roasting conditions and its effect on the final chromium recovery.