bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain Oxygenation During Thoracoscopic Repair of Long Gap Esophageal Atresia
Authors: Stolwijk Lisanne J. , van der Zee David C. , Tytgat Stefaan , van der Werff Desiree , Benders Manon J. N. L. , van Herwaarden Maud Y. A. , Lemmers Petra M. A. 
Published: January 2017

AbstractBackgroundElongation and repair of long gap esophageal atresia (LGEA) can be performed thoracoscopically, even directly after birth. The effect of thoracoscopic CO2‐insufflation on cerebral oxygenation (rScO2) during the consecutive thoracoscopic procedures in repair of LGEA was evaluated.MethodsProspective case series of five infants, with in total 16 repetitive thoracoscopic procedures. A CO2‐pneumothorax was installed with a pressure of maximum 5 mmHg and flow of 1 L/min. Parameters influencing rScO2 were monitored. For analysis 10 time periods of 10’ during surgery and in the perioperative period were selected.ResultsMedian gestational age was 35+3 [range 33+4 to 39+6] weeks; postnatal age at time of first procedure 4 [2–53] days and time of insufflation 127[22–425] min. Median rScO2 varied between 55 and 90%. Transient outliers in cerebral oxygenation were observed in three patients. In Patient 2 oxygenation values below 55% occurred during a low MABP and Hb < 6 mmol/L. The rScO2 increased after erythrocytes transfusion. Patient 5 also showed a rScO2 of 50% with a Hb <6 mmol/L during all procedures, except for a substantial increase during a high paCO2 of 60 mmHg. Patient 4 had a rScO2 > 85% during the first procedure with a concomitant high FiO2 > 45%. All parameters recovered during the surgical course.ConclusionsThis prospective case series of NIRS during consecutive thoracoscopic repair of LGEA showed that cerebral oxygenation remained stable. Transient outliers in rScO2 occurred during changes in hemodynamic or respiratory parameters and normalized after interventions of the anesthesiologist. This study underlines the importance of perioperative neuromonitoring and the close collaboration between pediatric surgeon, anesthesiologist and neonatologist.
Calcium Hypothesis of Alzheimer's disease and brain aging: A framework for integrating new evidence into a comprehensive theory of pathogenesis
Authors:   Alzheimer's Association Calcium Hypothesis Workgroup, Khachaturian Zaven S. 
Published: January 2017

AbstractThis article updates the Calcium Hypothesis of Alzheimer's disease and brain aging on the basis of emerging evidence since 1994 (The present article, with the subtitle “New evidence for a central role of Ca2+ in neurodegeneration,” includes three appendices that provide context and further explanations for the rationale for the revisions in the updated hypothesis—the three appendices are as follows: Appendix I “Emerging concepts on potential pathogenic roles of [Ca2+],” Appendix II “Future studies to validate the central role of dysregulated [Ca2+] in neurodegeneration,” and Appendix III “Epilogue: towards a comprehensive hypothesis.”) (Marx J. Fresh evidence points to an old suspect: calcium. Science 2007; 318:384–385). The aim is not only to re‐evaluate the original key claims of the hypothesis with a critical eye but also to identify gaps in knowledge required to validate relevant claims and delineate additional studies and/or data that are needed. Some of the key challenges for this effort included examination of questions regarding (1) the temporal and spatial relationships of molecular mechanisms that regulate neuronal calcium ion (Ca2+), (2) the role of changes in concentration of calcium ion [Ca2+] in various subcellular compartments of neurons, (3) how alterations in Ca2+ signaling affect the performance of neurons under various conditions, ranging from optimal functioning in a healthy state to conditions of decline and deterioration in performance during aging and in disease, and (4) new ideas about the contributions of aging, genetic, and environmental factors to the causal relationships between dysregulation of [Ca2+] and the functioning of neurons (see Appendices I and II). The updated Calcium Hypothesis also includes revised postulates that are intended to promote further crucial experiments to confirm or reject the various predictions of the hypothesis (see Appendix III).
On the receptivity of aerofoil tonal noise: an adjoint analysis
Authors: Fosas de Pando Miguel , Schmid Peter J. , Sipp Denis 
Published: January 2017

For moderate-to-high Reynolds numbers, aerofoils are known to produce substantial levels of acoustic radiation, known as tonal noise, which arises from a complex interplay between laminar boundary-layer instabilities, trailing-edge acoustic scattering and upstream receptivity of the boundary layers on both aerofoil surfaces. The resulting acoustic spectrum is commonly characterised by distinct equally spaced peaks encompassing the frequency range of convectively amplified instability waves in the pressure-surface boundary layer. In this work, we assess the receptivity and sensitivity of the flow by means of global stability theory and adjoint methods which are discussed in light of the spatial structure of the adjoint global modes, as well as the wavemaker region. It is found that for the frequency range corresponding to acoustic tones the direct global modes capture the growth of instability waves on the suction surface and the near wake together with acoustic radiation into the far field. Conversely, it is shown that the corresponding adjoint global modes, which capture the most receptive region in the flow to external perturbations, have compact spatial support in the pressure surface boundary layer, upstream of the separated flow region. Furthermore, we find that the relative spatial amplitude of the adjoint modes is higher for those modes whose real frequencies correspond to the acoustic peaks. Finally, analysis of the wavemaker region points at the pressure surface near 30 % of the chord as the preferred zone for the placement of actuators for flow control of tonal noise.
Two- and three-dimensional instabilities in the wake of a circular cylinder near a moving wall
Authors: Jiang Hongyi , Cheng Liang , Draper Scott , An Hongwei 
Published: January 2017

Two-dimensional (2D) and three-dimensional (3D) instabilities in the wake of a circular cylinder placed near to a moving wall are investigated using direct numerical simulation (DNS). The study covers a parameter space spanning a non-dimensional gap ratio ($G^{\ast }$) between 0.1 to 19.5 and Reynolds number ($Re$) up to 300. Variations in the flow characteristics with $Re$ and $G^{\ast }$ are studied, and their correlations with the hydrodynamic forces on the cylinder are investigated. It is also found that the monotonic increase of the critical $Re$ for 2D instability ($Re_{cr2D}$) with decreasing $G^{\ast }$ is influenced by variations in the mean flow rate around the cylinder, the confinement of the near-wake flow by the plane wall and the characteristics of the shear layer formed above the moving wall directly below the cylinder. The first factor destabilizes the wake flow at a moderate $G^{\ast }$ while the latter two factors stabilize the wake flow with decreasing $G^{\ast }$. In terms of 3D instability, the flow transition sequence of ‘2D steady $\rightarrow$ 3D steady $\rightarrow$ 3D unsteady’ for small gap ratios is analysed at $G^{\ast }=0.2$. It is found that the 3D steady and 3D unsteady flows are triggered by Mode C instability due to wall proximity. However, the Mode C structure is not sustained indefinitely, since interference with the shear layer leads to other 3D steady and unsteady flow structures.
Vortex dynamics for flow over a circular cylinder in proximity to a wall
Authors: He Guo-Sheng , Wang Jin-Jun , Pan Chong , Feng Li-Hao , Gao Qi , Rinoshika Akira 
Published: January 2017

The dynamics of vortical structures in flow over a circular cylinder in the vicinity of a flat plate is investigated using particle image velocimetry (PIV). The cylinder is placed above the flat plate with its axis parallel to the wall and normal to the flow direction. The Reynolds number $Re_{D}$ based on the cylinder diameter $D$ is 1072 and the gap $G$ between the cylinder and the flat plate is varied from gap-to-diameter ratio $G/D=0$ to $G/D=3.0$. The flow statistics and vortex dynamics are strongly dependent on the gap ratio $G/D$. Statistics show that as the cylinder comes close to the wall ($G/D\leqslant 2.0$), the cylinder wake becomes more and more asymmetric and a boundary layer separation is induced on the flat plate downstream of the cylinder. The wake vortex shedding frequency increases with decreasing $G/D$ until a critical gap ratio (about $G/D=0.25$) below which the vortex shedding is irregular. The deflection of the gap flow away from the wall and its following interaction with the upper shear layer may be the cause of the higher shedding frequency. The vortex dynamics is investigated based on the phase-averaged flow field and virtual dye visualization in the instantaneous PIV velocity field. It is revealed that when the cylinder is close to the wall ($G/D=2.0$), the cylinder wake vortices can periodically induce secondary spanwise vortices near the wall. As the cylinder approaches the wall ($G/D=1.0$) the secondary vortex can directly interact with the lower wake vortex, and a further approaching of the cylinder ($G/D=0.5$) can result in more complex interactions among the secondary vortex, the lower wake vortex and the upper wake vortex. The breakdown of vortices into filamentary debris during vortex interactions is clearly revealed by the coloured virtual dye visualizations. For $G/D<0.25$, the lower shear layer is strongly inhibited and only the upper shear layer can shed vortices. Investigation of the vortex formation, evolution and interaction in the flow promotes the understanding of the flow physics for different gap ratios.
AMS 14C Dating of Materials Recovered from the Tunnel under the Temple of the Feathered Serpent in Teotihuacan, Mexico
Authors: Gómez-Chávez S , Solís C , Gazzola J , Chávez-Lomelí E R , Mondragón M A , Rodríguez-Ceja M , Martínez-Carrillo M A 
Published: January 2017

AbstractIn 2003, flooding occurred in the Ciudadela (Citadel) of Teotihuacan and saltpeter began to damage the Temple of the Feathered Serpent. Work done to solve this problem led to one of the most important archaeological discoveries made in this site in recent years: an intact tunnel sealed for more than a thousand years. The project created to study the tunnel was named Tlalocan or Path to the Underworld. More than 60,000 objects have been recovered after years of exploration and removing huge amounts of soil and stones. This paper presents the first results of accelerator mass spectrometry (AMS) radiocarbon dating performed on some of those materials recovered from the tunnel. With these findings, in combination with the archaeological data, based on stratigraphy and ceramic typology, a chronology of several events is proposed concerning the construction phases and ceremonial use, as well as partial and definitive closures of the tunnel. Every closure was accompanied by a deliberate and structured deposition of offerings and ritual refuse along the tunnel. The range of ages that covers the Bayesian calibration of samples collected along the tunnel is around 115 yr, from AD 125 to 240. Material collected at the surface of the chamber located at the end of the tunnel and under the pyramid gave ages in the interval between AD 400 and 534. All samples analyzed fall within the interval of time that covers the period of occupation of Teotihuacan.
Louis J. Lanzerotti Receives 2016 William Kaula Award
Authors:   AGU
Published: January 2017

Louis J. Lanzerotti received the 2016 William Kaula Award at the American Geophysical Union Fall Meeting Honors Ceremony, held on 14 December 2016 in San Francisco, Calif. The award honors an individual for "unselfish service to the scientific community through extraordinary dedication to, and exceptional efforts on behalf of, the Union's publications program."
AIF-independent parthanatos in the pathogenesis of dry age-related macular degeneration
Authors: Jang Ki-Hong , Do Yun-Ju , Son Dongwon , Son Eunji , Choi Jun-Sub , Kim Eunhee 
Published: January 2017

AbstractCell death of retinal pigment epithelium (RPE) is characterized as an essential late-stage phenomenon of dry age-related macular degeneration (AMD). The aim of this study was to elucidate the molecular mechanism underlying RPE cell death after exposure to oxidative stress, which occurs often because of the anatomical location of RPE cells. ARPE-19, an established RPE cell line, exhibited necrotic features involving poly (ADP-ribose) polymerase-1 (PARP-1) activation in response to hydrogen peroxide (H2O2). ARPE-19 cells were resistant to H2O2 when PARP-1 was depleted using siRNA or inhibited by a pharmacological inhibitor of PARP-1, olaparib. Our data suggest a causal relationship between PARP-1 activation and ARPE-19 cell death in response to H2O2. Next, we investigated downstream molecular events in PARP-1 activation. Increased mitochondrial depolarization, mitochondrial fission and alterations of the cellular energy dynamics with reduced NAD+ and ATP were observed in H2O2-treated ARPE-19 cells. H2O2-triggered mitochondrial dysfunction was inhibited by olaparib. Nevertheless, translocation of apoptosis-inducing factor (AIF), a biochemical signature for PARP-1-dependent cell death (parthanatos), was not observed in our study. Moreover, the depletion of AIF did not affect the amplitude of cell death, demonstrating the lack of a role for AIF in the death of ARPE-19 cells in response to H2O2. This feature distinguishes the type of death observed in this study from canonical parthanatos. Next, we examined the in vivo role of PARP-1 in a dry AMD animal model system. Histological analysis of the outer nuclear layer in the mouse retina revealed protection against sodium iodate (SI) following treatment with olaparib. Moreover, retina fundus and electroretinograms also confirmed such a protective effect in the SI-treated rabbit. Collectively, we report that AIF-independent PARP-1-dependent necrosis constitutes a major mechanism of RPE cell death leading to retinal degeneration in dry AMD.
MicroRNA-494 inhibits breast cancer progression by directly targeting PAK1
Authors: Zhan Meng-Na , Yu Xiao-Ting , Tang Jun , Zhou Ci-Xiang , Wang Chen-Long , Yin Qian-Qian , Gong Xiu-Feng , He Ming , He Jian-Rong , Chen Guo-Qiang , Zhao Qian 
Published: January 2017

AbstractMicroRNA (miRNA) is involved in the progression and metastasis of diverse human cancers, including breast cancer, as strong evidence has been found that miRNAs can act as oncogenes or tumor suppressor genes. Here, we show that miR-494 is decreased in human breast cancer specimens and breast cancer cell lines. Ectopic expression of miR-494 in basal-like breast cancer cell lines MDA-MB-231-LUC-D2H3LN and BT-549 inhibits clonogenic ability and metastasis-relevant traits in vitro. Moreover, ectopic expression of miR-494 suppresses neoplasm initiation as well as pulmonary metastasis in vivo. Further studies have identified PAK1, as a direct target gene of miR-494, contributes to the functions of miR-494. Remarkably, the expression of PAK1 is inversely correlated with the level of miR-494 in human breast cancer samples. Furthermore, re-expression of PAK1 partially reverses miR-494-mediated proliferative and clonogenic inhibition as well as migration and invasion suppression in breast cancer cells. Taken together, these findings highlight an important role for miR-494 in the regulation of progression and metastatic potential of breast cancer and suggest a potential application of miR-494 in breast cancer treatment.
Inhibition of Stat3 signaling pathway by nifuroxazide improves antitumor immunity and impairs colorectal carcinoma metastasis
Authors: Ye Ting-Hong , Yang Fang-Fang , Zhu Yong-Xia , Li Ya-Li , Lei Qian , Song Xue-Jiao , Xia Yong , Xiong Ying , Zhang Li-Dan , Wang Ning-Yu , Zhao Li-Feng , Gou Hong-Feng , Xie Yong-Mei , Yang Sheng-Yong , Yu Luo-Ting , Yang Li , Wei Yu-Quan 
Published: January 2017

AbstractColorectal carcinoma (CRC) is the one of the most common cancers with considerable metastatic potential, explaining the need for new drug candidates that inhibit tumor metastasis. The signal transducers and activators of the transcription 3 (Stat3) signaling pathway has an important role in CRC and has been validated as a promising anticancer target for CRC therapy. In the present study, we report our findings on nifuroxazide, an antidiarrheal agent identified as an inhibitor of Stat3. Our studies showed that nifuroxazide decreased the viability of three CRC cell lines and induced apoptosis of cancer cells in a concentration-dependent manner. Moreover, western blot analysis demonstrated that the occurrence of its apoptosis was correlated with the activation of Bax and cleaved caspase-3, and decreased the expression of Bcl-2. In addition, nifuroxazide markedly impaired CRC cell migration and invasion by downregulating phosphorylated-Stat3Tyr705, and also impaired the expression of matrix metalloproteinases (MMP-2 and MMP-9). Furthermore, our studies showed that nifuroxazide also significantly inhibited the tumor metastasis in lung and abdomen metastasis models of colon cancer. Meanwhile, nifuroxazide functionally reduced the proliferation index, induced tumor apoptosis and impaired metastasis. Notably, nifuroxazide reduced the number of myeloid-derived suppressor cells in the blood, spleens and tumors, accompanied by the increased infiltration of CD8+ T cells in the tumors. Importantly, a marked decrease in the number of M2-type macrophages in tumor in the abdomen metastasis model was also observed. Taken together, our results indicated that nifuroxazide could effectively inhibit tumor metastasis by mediating Stat3 pathway and it might have a therapeutic potential for the treatment of CRC.
c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis
Authors: Zhang Jia , Wang Suili , Jiang Bin , Huang Lihong , Ji Zhiliang , Li Xiaotong , Zhou Huamin , Han Aidong , Chen Ai , Wu Yanan , Ma Huanhuan , Zhao Wentao , Zhao Qingwen , Xie Changchuan , Sun Xiaoyan , Zhou Yanming , Huang Huiying , Suleman Muhammad , Lin Furong , Zhou Lin , Tian Fang , Jin Meijun , Cai Yana , Zhang Nan , Li Qinxi 
Published: January 2017

AbstractIt is well known that c-Src has important roles in tumorigenesis. However, it remains unclear whether c-Src contributes to metabolic reprogramming. Here we find that c-Src can interact with and phosphorylate hexokinases HK1 and HK2, the rate-limiting enzymes in glycolysis. Tyrosine phosphorylation dramatically increases their catalytic activity and thus enhances glycolysis. Mechanistically, c-Src phosphorylation of HK1 at Tyr732 robustly decreases its Km and increases its Vmax by disrupting its dimer formation. Mutation in c-Src phosphorylation site of either HK1 or HK2 remarkably abrogates the stimulating effects of c-Src on glycolysis, cell proliferation, migration, invasion, tumorigenesis and metastasis. Due to its lower Km for glucose, HK1 rather than HK2 is required for tumour cell survival when glucose is scarce. Importantly, HK1-Y732 phosphorylation level remarkably correlates with the incidence and metastasis of various clinical cancers and may serve as a marker to predict metastasis risk of primary cancers.
Layer-specificity in the effects of attention and working memory on activity in primary visual cortex
Authors: van Kerkoerle Timo , Self Matthew W. , Roelfsema Pieter R. 
Published: January 2017

AbstractNeuronal activity in early visual cortex depends on attention shifts but the contribution to working memory has remained unclear. Here, we examine neuronal activity in the different layers of the primary visual cortex (V1) in an attention-demanding and a working memory task. A current-source density analysis reveales top-down inputs in the superficial layers and layer 5, and an increase in neuronal firing rates most pronounced in the superficial and deep layers and weaker in input layer 4. This increased activity is strongest in the attention task but it is also highly reliable during working memory delays. A visual mask erases the V1 memory activity, but it reappeares at a later point in time. These results provide new insights in the laminar circuits involved in the top-down modulation of activity in early visual cortex in the presence and absence of visual stimuli.
A global distributed basin morphometric dataset
Authors: Shen Xinyi , Anagnostou Emmanouil N. , Mei Yiwen , Hong Yang 
Published: January 2017

AbstractBasin morphometry is vital information for relating storms to hydrologic hazards, such as landslides and floods. In this paper we present the first comprehensive global dataset of distributed basin morphometry at 30 arc seconds resolution. The dataset includes nine prime morphometric variables; in addition we present formulas for generating twenty-one additional morphometric variables based on combination of the prime variables. The dataset can aid different applications including studies of land-atmosphere interaction, and modelling of floods and droughts for sustainable water management. The validity of the dataset has been consolidated by successfully repeating the Hack’s law.
Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
Authors: Stuchlý Jan , Kanderová Veronika , Vlková Marcela , Heřmanová Ivana , Slámová Lucie , Pelák Ondřej , Taraldsrud Eli , Jílek Dalibor , Králíčková Pavlína , Fevang Børre , Trková Marie , Hrušák Ondřej , Froňková Eva , Šedivá Anna , Litzman Jiří , Kalina Tomáš 
Published: January 2017

AbstractCommon variable immunodeficiency (CVID) is a heterogeneous group of diseases. Our aim was to define sub-groups of CVID patients with similar phenotypes and clinical characteristics. Using eight-color flow cytometry, we analyzed both B- and T-cell phenotypes in a cohort of 88 CVID patients and 48 healthy donors. A hierarchical clustering of probability binning “bins” yielded a separate cluster of 22 CVID patients with an abnormal phenotype. We showed coordinated proportional changes in naïve CD4+ T-cells (decreased), intermediate CD27− CD28+ CD4+ T-cells (increased) and CD21low B-cells (increased) that were stable for over three years. Moreover, the lymphocytes’ immunophenotype in this patient cluster exhibited features of profound immunosenescence and chronic activation. Thrombocytopenia was only found in this cluster (36% of cases, manifested as Immune Thrombocytopenia (ITP) or Evans syndrome). Clinical complications more frequently found in these patients include lung fibrosis (in 59% of cases) and bronchiectasis (55%). The degree of severity of these symptoms corresponded to more deviation from normal levels with respect to CD21low B-cells, naïve CD4+ and CD27− CD28+ CD4+ T-cells. Next-generation sequencing did not reveal any common genetic background. We delineate a subgroup of CVID patients with activated and immunosenescent immunophenotype of lymphocytes and distinct set of clinical complications without common genetic background.