bluuebunny/crossref_metadata_2025_split huggingface dataset:
Winter Lightning to the Lee of Lake Ontario: The Lake-Effect Electrification (LEE) Field Campaign
Authors: Steiger Scott M. , Bruning Eric C. , Chmielewski Vanna C. , Stano Geoffrey , Trostel John , Calhoun Kristin M. , Jesmonth Kaitlyn R. , Lamsma Bee , Lang Timothy , Laurinaitis Shaun , Losego Jessica , Ringhausen Jacquelyn S. , Stock Michael , Wang Yonggang , Waugh Sean M. , Weiss Stephanie A. , Weist Thomas , White Thomas 
Published: September 2024

Abstract
The National Science Foundation–sponsored Lake-Effect Electrification (LEE) field campaign intensive observation periods occurred between November and early February 2022–23 across the eastern Lake Ontario region. Project LEE documented, for the first time, the total lightning and electrical charge structures of lake-effect storms and the associated storm environment using a lightning mapping array (LMA), a mobile dual-polarization X-band radar, and balloon-based soundings that measured vertical profiles of temperature, humidity, wind, electric field, and hydrometeor types. LEE also observed abundant wind turbine-initiated lightning, which is climatologically more likely during the winter. The frequent occurrence of intense lake-effect storms and the proximity of a wind farm with nearly 300 turbines each more than 100 m tall to the lee of Lake Ontario provided an ideal laboratory for this study. The field project involved many undergraduate (>20) and graduate students. Some foreseen and unforeseen challenges included clearing the LMA solar panels of snow and continuous operation in low-sunlight conditions, large sonde balloons prematurely popping due to extremely cold conditions, sonde line breaking, recovering probes in deep snow in heavily forested areas, vehicles getting stuck in the snowpack, and an abnormally dry season for parts of the LEE domain. In spite of these difficulties, a dataset was collected in multiple lake-effect snowstorms (11 observation periods) and one extratropical cyclone snowstorm that clarifies the electrical structure of these systems. A key finding was the existence of a near-surface substantial positive charge layer (1 nC m−3) near the shoreline during lake-effect thunderstorms.
Characteristics of lymphoedema, in particular midline lymphoedema, after treatment for prostate cancer: a retrospective study
Authors: Van Calster Charlotte , Everaerts Wouter , Geraerts Inge , De Groef An , Heroes An-Kathleen , De Vrieze Tessa , Alar Ceyhun , Devoogdt Nele 
Published: September 2024

Abstract
Background
Patients undergoing treatment for prostate cancer may develop lymphoedema of the midline region. This has a substantial impact on a patient’s quality of life and its diagnosis is often delayed or missed. Therefore, the purpose of this study is to compare the characteristics of patients with leg and midline lymphoedema to patients with only leg lymphoedema.

Methods
We retrospectively collected patient-, cancer-, lymphoedema- and lymphoedema treatment-related data of 109 men with lymphoedema after treatment for prostate cancer. First, 42 characteristics were compared between both groups. Second, factors predicting presence of midline lymphoedema were explored by multivariable analyses.

Results
The mean age of the patients with lymphoedema was 68 ( ±7) years and mean BMI is 28 (±4) kg/m2. Median duration of lymphoedema before the first consultation was 27 (9;55) months. Based on univariable analyses, patients with leg and midline lymphoedema had more frequently upper leg lymphoedema (89% (31/35) vs. 69% (51/74), p = 0.026), skin fibrosis (34% (12/35) vs. 16% (12/74), p = 0.034) and lymphatic reconstructive surgery (9% (3/35) vs. 0% (0/71), p = 0.020) than patients with only leg lymphoedema. Additionally, patients with leg and midline lymphoedema reported less frequently lower leg lymphoedema (77% (27/35) vs. 95% (70/74), p = 0.007). Based on the multivariable analysis, not having lower leg lymphoedema, skin fibrosis, performing self-bandaging and self-manual lymphatic drainage appear to be predictors for having midline lymphoedema.

Conclusions
If patients with lymphoedema after prostate cancer do not have lower leg lymphoedema, have skin fibrosis, perform self-bandaging or self-manual lymphatic drainage, they possibly have midline lymphoedema.
Perspectives on a peer-driven intervention to promote pre-exposure prophylaxis (PrEP) uptake among men who have sex with men in southern New England: a qualitative study
Authors: Tao Jun , Parent Hannah , Karki Ishu , Martin Harrison , Marshall Sarah Alexandra , Kapadia Jhanavi , Nunn Amy S. , Marshall Brandon D. L. , Raymond Henry F. , Mena Leandro , Chan Philip A. 
Published: September 2024

Abstract
Background
Pre-exposure prophylaxis (PrEP) is a highly effective pharmaceutical intervention that prevents HIV infection, but PrEP uptake across the US has been slow among men who have sex with men (MSM), especially among Black/African American (B/AA) and Hispanic /Latino (H/L) MSM. This study investigates the acceptability and essential components of a peer-driven intervention (PDI) for promoting PrEP uptake among MSM, with a specific focus on B/AA and H/L communities.

Methods
We conducted 28 semi-structured, qualitative interviews with MSM in southern New England to explore the components of a PDI, including attitudes, content, and effective communication methods. A purposive sampling strategy was used to recruit diverse participants who reflect the communities with the highest burden of HIV infection.

Results
Of 28 study participants, the median age was 28 years (interquartile range [IQR]: 25, 35). The sample comprised B/AA (39%, n = 11) and H/L (50%, n = 14) individuals. Notably, nearly half of the participants (46%) were current PrEP users. We found that many participants were in favor of using a PDI approach for promoting PrEP. Additionally, several participants showed interest in becoming peer educators themselves. They emphasized the need for strong communication skills to effectively teach others about PrEP. Moreover, participants noted that peer education should cover key topics like how PrEP works, how effective it is, and any possible side effects.

Conclusions
Our study shows that effective PDIs, facilitated by well-trained peers knowledgeable about PrEP, could enhance PrEP uptake among MSM, addressing health disparities and potentially reducing HIV transmission in B/AA and H/L communities.
Dengue NS1 interaction with lipids alters its pathogenic effects on monocyte derived macrophages
Authors: Dayarathna Shashika , Senadheera Bhagya , Jeewandara Chandima , Dissanayake Madushika , Bary Farha , Ogg Graham S. , Malavige Gathsaurie Neelika 
Published: September 2024

Abstract
Background
While dengue NS1 antigen has been shown to be associated with disease pathogenesis in some studies, it has not been linked in other studies, with the reasons remaining unclear. NS1 antigen levels in acute dengue are often associated with increased disease severity, but there has been a wide variation in results based on past dengue infection and infecting dengue virus (DENV) serotype. As NS1 engages with many host lipids, we hypothesize that the type of NS1-lipid interactions alters its pathogenicity.

Methods
Primary human monocyte derived macrophages (MDMs) were co-cultured with NS1 alone or with HDL, LDL, LPS and/or platelet activating factor (PAF) from individuals with a history of past dengue fever (DF = 8) or dengue haemorrhagic fever (DHF = 8). IL-1β levels were measured in culture supernatants, and gene expression analysis carried out in MDMs. Monocyte subpopulations were assessed by flow cytometry. Hierarchical cluster analysis with Euclidean distance calculations were used to differentiate clusters. Differentially expressed variables were extracted and a classifier model was developed to differentiate between past DF and DHF.

Results
Significantly higher levels of IL-1β were seen in culture supernatants when NS1 was co-cultured with LDL (p = 0.01, median = 45.69 pg/ml), but lower levels when NS1 was co-cultured with HDL (p = 0.05, median = 4.617 pg/ml). MDMs of those with past DHF produced higher levels of IL-1β when NS1 was co-cultured with PAF (p = 0.02). MDMs of individuals with past DHF, were significantly more likely to down-regulate RPLP2 gene expression when macrophages were co-cultured with either PAF alone, or NS1 combined with PAF, or NS1 combined with LDL. When NS1 was co-cultured with PAF, HDL or LDL two clusters were detected based on IL10 expression, but these did not differentiate those with past DF or DHF.

Conclusions
As RPLP2 is important in DENV replication, regulating cellular stress responses and immune responses and IL-10 is associated with severe disease, it would be important to further explore how differential expression of RPLP2 and IL-10 could lead to disease pathogenesis based on NS1 and lipid interactions.
A new modular neuroprosthesis suitable for hybrid FES-robot applications and tailored assistance
Authors: Gil-Castillo Javier , Herrera-Valenzuela Diana , Torricelli Diego , Gil-Agudo Ángel , Opisso Eloy , Vidal Joan , Font-Llagunes Josep M. , del-Ama Antonio J. , Moreno Juan C. 
Published: September 2024

AbstractBackgroundTo overcome the application limitations of functional electrical stimulation (FES), such as fatigue or nonlinear muscle response, the combination of neuroprosthetic systems with robotic devices has been evaluated, resulting in hybrid systems that have promising potential. However, current technology shows a lack of flexibility to adapt to the needs of any application, context or individual. The main objective of this study is the development of a new modular neuroprosthetic system suitable for hybrid FES-robot applications to meet these needs.MethodsIn this study, we conducted an analysis of the requirements for developing hybrid FES-robot systems and reviewed existing literature on similar systems. Building upon these insights, we developed a novel modular neuroprosthetic system tailored for hybrid applications. The system was specifically adapted for gait assistance, and a technological personalization process based on clinical criteria was devised. This process was used to generate different system configurations adjusted to four individuals with spinal cord injury or stroke. The effect of each system configuration on gait kinematic metrics was analyzed by using repeated measures ANOVA or Friedman’s test.ResultsA modular NP system has been developed that is distinguished by its flexibility, scalability and personalization capabilities. With excellent connection characteristics, it can be effectively integrated with robotic devices. Its 3D design facilitates fitting both as a stand-alone system and in combination with other robotic devices. In addition, it meets rigorous requirements for safe use by incorporating appropriate safety protocols, and features appropriate battery autonomy, weight and dimensions. Different technological configurations adapted to the needs of each patient were obtained, which demonstrated an impact on the kinematic gait pattern comparable to that of other devices reported in the literature.ConclusionsThe system met the identified technical requirements, showcasing advancements compared to systems reported in the literature. In addition, it demonstrated its versatility and capacity to be combined with robotic devices forming hybrids, adapting well to the gait application. Moreover, the personalization procedure proved to be useful in obtaining various system configurations tailored to the diverse needs of individuals.
Targeting lipid droplets and lipid droplet-associated proteins: a new perspective on natural compounds against metabolic diseases
Authors: Jiang Xinyue , Wang Hongzhan , Nie Kexin , Gao Yang , Chen Shen , Tang Yueheng , Wang Zhi , Su Hao , Dong Hui 
Published: September 2024

Abstract
Background
Lipid droplet (LD) is a metabolically active organelle, which changes dynamically with the metabolic state and energy requirements of cells. Proteins that either insert into the LD phospholipid monolayer or are present in the cytoplasm, playing a crucial role in lipid homeostasis and signaling regulation, are known as LD-associated proteins.

Methods
The keywords “lipid droplets” and “metabolic diseases” were used to obtain literature on LD metabolism and pathological mechanism. After searching databases including Scopus, OVID, Web of Science, and PubMed from 2013 to 2024 using terms like “lipid droplets”, “lipid droplet-associated proteins”, “fatty liver disease”, “diabetes”, “diabetic kidney disease”, “obesity”, “atherosclerosis”, “hyperlipidemia”, “natural drug monomers” and “natural compounds”, the most common natural compounds were identified in about 954 articles. Eventually, a total of 91 studies of 10 natural compounds reporting in vitro or in vivo studies were refined and summarized.

Results
The most frequently used natural compounds include Berberine, Mangostin, Capsaicin, Caffeine, Genistein, Epigallocatechin-3-gallate, Chlorogenic acid, Betaine, Ginsenoside, Resveratrol. These natural compounds interact with LD-associated proteins and help ameliorate abnormal LDs in various metabolic diseases.

Conclusion
Natural compounds involved in the regulation of LDs and LD-associated proteins hold promise for treating metabolic diseases. Further research into these interactions may lead to new therapeutic applications.

Graphical Abstract
Single-cell transcriptional atlas of tumor-associated macrophages in breast cancer
Authors: Zhang Yupeng , Zhong Fan , Liu Lei 
Published: September 2024

Abstract
Background
The internal heterogeneity of breast cancer, notably the tumor microenvironment (TME) consisting of malignant and non-malignant cells, has been extensively explored in recent years. The cells in this complex cellular ecosystem activate or suppress tumor immunity through phenotypic changes, secretion of metabolites and cell-cell communication networks. Macrophages, as the most abundant immune cells within the TME, are recruited by malignant cells and undergo phenotypic remodeling. Tumor-associated macrophages (TAMs) exhibit a variety of subtypes and functions, playing significant roles in impacting tumor immunity. However, their precise subtype delineation and specific function remain inadequately defined.

Methods
The publicly available single-cell transcriptomes of 49,141 cells from eight breast cancer patients with different molecular subtypes and stages were incorporated into our study. Unsupervised clustering and manual cell annotation were employed to accurately classify TAM subtypes. We then conducted functional analysis and constructed a developmental trajectory for TAM subtypes. Subsequently, the roles of TAM subtypes in cell-cell communication networks within the TME were explored using endothelial cells (ECs) and T cells as key nodes. Finally, analyses were repeated in another independent publish scRNA datasets to validate our findings for TAM characterization.

Results
TAMs are accurately classified into 7 subtypes, displaying anti-tumor or pro-tumor roles. For the first time, we identified a new TAM subtype capable of proliferation and expansion in breast cancer-TUBA1B+ TAMs playing a crucial role in TAMs diversity and tumor progression. The developmental trajectory illustrates how TAMs are remodeled within the TME and undergo phenotypic and functional changes, with TUBA1B+ TAMs at the initial point. Notably, the predominant TAM subtypes varied across different molecular subtypes and stages of breast cancer. Additionally, our research on cell-cell communication networks shows that TAMs exert effects by directly modulating intrinsic immunity, indirectly regulating adaptive immunity through T cells, as well as influencing tumor angiogenesis and lymphangiogenesis through ECs.

Conclusions
Our study establishes a precise single-cell atlas of breast cancer TAMs, shedding light on their multifaceted roles in tumor biology and providing resources for targeting TAMs in breast cancer immunotherapy.
Identification and functional analysis of C-type lectin from mosquito Aedes albopictus in response to dengue virus infection
Authors: Gao Sheng , Xu Haodong , Li Hongbo , Feng Xiao , Zhou Jitao , Guo Renxian , Liang Zihan , Ding Jinying , Li Xin , Huang Yijia , Liu Wenquan , Liang Shaohui 
Published: September 2024

Abstract
Background
C-type lectins (CTLs) are a large family of proteins with sugar-binding activity. CTLs contain an evolutionarily conserved C-type lectin domain (CTLD) that binds microbial carbohydrates in a calcium-dependent manner, thereby playing a key role in both microbial pathogenesis and innate immune responses. Aedes albopictus is an important vector for transmitting dengue virus (DENV) worldwide. Currently, the molecular characteristics and functions of CTLs in Ae. albopictus are largely unknown.

Methods
Transcripts encoding CTL proteins in the Ae. albopictus genome assembly were analyzed via sequence blast. Phylogenetic analysis and molecular characterization were performed to identify the functional domains of the CTLs. Quantitative analysis was performed to determine the gene expression features of CTLs during mosquito development and in different tissues of female adults after blood feeding. In addition, the functional role of CTLs in response to DENV infection was investigated in Ae. albopictus mosquito cells.

Results
We identified 39 transcripts encoding CTL proteins in the Ae. albopictus transcriptome. Aedes albopictus CTLs are classified into three groups based on the number of CTLDs and the domain architecture. These included 29 CTL-Ss (single-CTLDs), 1 immulectins (dual-CTLD) and 9 CTL-Xs (CTLDs with other domains). Phylogenetic analysis and structural modeling indicated that CTLs in Ae. albopictus are highly conserved with the homologous CTLs in Aedes aegypti. The expression profile assay revealed differential expression patterns of CTLs in both developmental stages and in adult female tissues. Knockdown and overexpression of three CTLs (CTL-S12, S17 and S19) confirmed that they can promote dengue virus infection in Ae. albopictus cells.

Conclusions
The CTL genes in Ae. albopictus mosquito and other mosquito species are evolutionarily conserved and exhibit different developmental and tissue expression features. The functional assay indicated that three CTLs in Ae. albopictus mosquitoes are involved in promoting dengue virus infection. Our study revealed that CTLs play important roles in both the physiological processes and viral infection in mosquito vectors.

Graphical Abstract
Inhibitory effect of human indoleamine 2,3-dioxygenase 1 (hIDO1) by kazinols of 1,3-diphenylpropane derivatives
Authors: Oh Taehoon , Jung Sunin , Oh Seon Min , Park Mi Hyeon , Kim Hyoung-Geun , Lee Su-Yeon , Ko Sung-Kyun , Ryu Hyung Won 
Published: September 2024

AbstractThis study focused on identifying and characterizing 1,3-diphenylpropane derivatives from flavonoids that inhibit human indoleamine 2,3-dioxygenase 1 (hIDO1) enzymes, which play a role in immune regulation and are associated with various diseases. A series of isolated metabolites (1–7) demonstrated modest to high inhibition of hIDO1, with binding degree values ranging from 26.31 to 72.17%. In particular, during a target-based screening of natural products using hIDO1, kazinol J (6, a 1,3-diphenylpropane derivative) was found to potently inhibit hIDO1, with a binding degree of 72.17% at 1 ppm. Kazinol J (6) showed concentration-dependent and mixed inhibition kinetics and achieved slow and time-dependent inhibition of hIDO1. Additionally, docking simulations were performed to evaluate the inhibitory potential and binding interactions of the compounds with hIDO1. These findings suggest that these 1,3-diphenylpropane derivatives can serve as therapeutic agents for conditions involving hIDO1 dysregulation, such as cancer, autoimmune disorders, and infectious diseases.
Smallholder access to purchased seeds in the presence of pervasive market imperfections and rainfall shocks: panel data evidence from Malawi and Ethiopia
Authors: Makate Clifton , Angelsen Arild , Holden Stein Terje , Westengen Ola Tveitereid 
Published: September 2024

AbstractSeed purchasing enables farmers to respond to adverse events that may cause chronic and temporary seed insecurity by allowing them to exploit opportunities associated with accessing new seeds. However, as with other inputs, seed purchasing is complicated by pervasive market imperfections and climate risk common in Sub-Sahara Africa. This study uses balanced household panel data for Malawi (2010–2018) and Ethiopia (2012–2016) and applies dynamic random effects Probit and Tobit models to assess how seed purchase decisions are affected by earlier participation in the market, lagged rainfall shocks, and historical climate variables. Our findings show that there are nonlinear effects of lagged seed purchase decisions on subsequent decisions with strong initial effects (weakening over time). For instance, initial maize seed purchase decisions are associated with about 11 and 22% higher probability of purchase and 1 and 2% higher shares of seed volumes purchased in later rounds in Malawi and Ethiopia, respectively. Seed purchase decisions also respond to climate variability and shocks. For instance, lagged drought shocks enhance subsequent maize purchase decisions in both countries. Historical average rainfall and temperature enhance maize seed purchase decisions in both countries. Overall, results point to state dependency on the demand side of the seed market, leading to selective access to purchased seeds. Also, seed purchase in smallholder farming is a liquidity and risk-dependent input choice. Policy efforts need to continue targeting reducing transaction costs and other barriers to entry into seed markets to enhance access to off-farm seed and support adaptation to rainfall shocks.
Modelling user notification scenarios in privacy policies
Authors: Kuznetsov Mikhail , Novikova Evgenia , Kotenko Igor 
Published: September 2024

AbstractThe processing of personal data gives a rise to many privacy concerns, and one of them is to ensure the transparency of data processing to end users. Usually this information is communicated to them using privacy policies. In this paper, the problem of user notification in case of data breaches and policy changes is addressed, besides an ontology-based approach to model them is proposed. To specify the ontology concepts and properties, the requirements and recommendations for the legislative regulations as well as existing privacy policies are evaluated. A set of SPARQL queries to validate the correctness and completeness of the proposed ontology are developed. The proposed approach is applied to evaluate the privacy policies designed by cloud computing providers and IoT device manufacturers. The results of the analysis show that the transparency of user notification scenarios presented in the privacy policies is still very low, and the companies should reconsider the notification mechanisms and provide more detailed information in privacy policies.