bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liberal intolerance in European education debates
Authors: Olsen Tore Vincents 
Published: November 2016

AbstractThe reaction against non‐western immigrants and especially Muslims has been analysed both in terms of an exclusionary civic nationalism and in terms of an assertive liberalism. Similar to exclusionary civic nationalism, assertive liberalism purports to defend liberal democratic principles and society against illiberal principles and forces predominantly represented by Muslims. This article argues that nationalism and liberalism are analytically distinguishable but difficult to disentangle empirically. It contends that a more detailed analysis of assertive liberalism can be obtained by subdividing it into four categories of liberal intolerance and demonstrates this by analysing six national debates on the accommodation of cultural and religious diversity in education. The analysis indicates that the nature of liberal intolerance understood as the combination of the four categories of liberal intolerance varies with the state tradition regarding religious neutrality of public institutions and the type of welfare state, but also that many liberal arguments for and against accommodation repeat themselves across national contexts.
Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+‐regulated Photoproteins of Different Organisms
Authors: Eremeeva Elena V. , Bartsev Sergey I. , van Berkel Willem J. H. , Vysotski Eugene S. 
Published: November 2016

AbstractUpon binding their metal ion cofactors, Ca2+‐regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+‐regulated photoproteins—aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata—demonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca2+‐regulated photoproteins.
Both JrWRKY2 and JrWRKY7 of Juglans regia mediate responses to abiotic stresses and abscisic acid through formation of homodimers and interaction
Authors: Yang G. , Zhang W. , Liu Z. , Yi‐Maer A.‐Y. , Zhai M. , Xu Z. 
Published: November 2016

Abstract

WRKY transcription factors belong to a large protein family that is involved in diverse developmental processes and abiotic stress responses. Currently, there is little understanding of the role of WRKY transcription factors in regulatory mechanisms in plants, especially in the protein‐protein interactions that are essential for biological regulatory functions and networks.

In the present study, yeast one‐hybrid, yeast two‐hybrid, transient expression and quantitative RT‐PCR were applied to investigate the potential characteristics of two WRKY proteins from Juglans regia, JrWRKY2 (GenBank Accession No. KU057089) and JrWRKY7 (GenBank Accession No. KP784651).

JrWRKY2 and JrWRKY7 can form homodimers and interact with each other. JrWRKY2 and JrWRKY7 can bind to W‐box motifs. Similarly high levels of transcription were found for JrWRKY2 and JrWRKY7 under NaCl and polyethylene glycol (PEG) stresses, as well as at different developmental stages, e.g., the pistil or terminal leaf. JrWRKY2 and JrWRKY7 were transiently overexpressed in an independent manner in the terminal leaf. Analyses of superoxide dismutase (SOD) and peroxidase (POD) activities, proline and malondialdehyde (MDA) contents, and electrolyte leakage rate showed that JrWRKY2 and JrWRKY7 overexpression improved plant tolerance to NaCl, PEG, abscisic acid, and cold stress. Additionally, JrWRKY2 and JrWRKY7 overexpression elevated transcription of SOD, POD, glutathione peroxidase (GPX), catalase (CAT), ascorbate peroxidase (APX), and MYB genes, but downregulated the expression of NAC.

Overall, the results demonstrate that JrWRKY2 and JrWRKY7 are dimeric proteins that can form functional homodimers and interact with each other and that they are involved in abiotic stress responses.
The mutation nrpb1‐A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF
Authors: Babiychuk Elena , Hoang Khai Trinh , Vandepoele Klaas , Van De Slijke Eveline , Geelen Danny , De Jaeger Geert , Obokata Junichi , Kushnir Sergei 
Published: November 2016

SummaryThe evolutionarily conserved 12‐subunit RNA polymerase II (Pol II) is a central catalytic component that drives RNA synthesis during the transcription cycle that consists of transcription initiation, elongation, and termination. A diverse set of general transcription factors, including a multifunctional TFIIF, govern Pol II selectivity, kinetic properties, and transcription coupling with posttranscriptional processes. Here, we show that TFIIF of Arabidopsis (Arabidopsis thaliana) resembles the metazoan complex that is composed of the TFIIFα and TFIIFβ polypeptides. Arabidopsis has two TFIIFβ subunits, of which TFIIFβ1/MAN1 is essential and TFIIFβ2/MAN2 is not. In the partial loss‐of‐function mutant allele man1‐1, the winged helix domain of Arabidopsis TFIIFβ1/MAN1 was dispensable for plant viability, whereas the cellular organization of the shoot and root apical meristems were abnormal. Forward genetic screening identified an epistatic interaction between the largest Pol II subunit nrpb1‐A325V variant and the man1‐1 mutation. The suppression of the man1‐1 mutant developmental defects by a mutation in Pol II suggests a link between TFIIF functions in Arabidopsis transcription cycle and the maintenance of cellular organization in the shoot and root apical meristems.
Genome Sequences of Malonate-Positive Cronobacter sakazakii Serogroup O:2, Sequence Type 64 Strains CDC 1121-73 and GK1025, Isolated from Human Bronchial Wash and a Powdered Infant Formula Manufacturing Plant
Authors: Chase H. R. , Gopinath G. R. , Gangiredla J. , Patel I. R. , Kothary M. H. , Carter L. , Sathyamoorthy V. , Lee B. , Park E. , Yoo Y. J. , Chung T. J. , Choi H. , Jun S. , Park J. , Jeong S. , Kim M. , Reich F. , Klein G. , Tall B. D. 
Published: November 2016

ABSTRACT

We introduce draft genome sequences of strains CDC1121-73 (human bronchial wash isolate) and GK1025 (powdered infant formula manufacturing facility isolate), which are both malonate-positive
Cronobacter sakazakii
serogroup O:2, sequence type 64. Assemblies for these strains have sizes of 4,442,307 and 4,599,266 bp and % G+C contents of 56.9 and 56.7, respectively.
Complete Genome Sequence of Pediococcus pentosaceus Strain wikim 20, Isolated from Korean Kimchi
Authors: Lee Se Hee , Jung Min Young , Park Boyeon , Sung-Oh Sohn , Park Hae Woong , Choi Hak-Jong , Lee Jong-Hee 
Published: November 2016

ABSTRACT

Pediococcus pentosaceus
strain wikim 20 is a lactic acid bacterium that was isolated from kimchi, a representative traditional Korean fermented food. Here, we announce the complete genome sequence of
P. pentosaceus
strain wikim 20 consisting of a 1,830,629-bp chromosome and provide a description of its annotation.
Draft Genome Sequence for the Type Strain Vulcanibacillus modesticaldus BR, a Strictly Anaerobic, Moderately Thermophilic, and Nitrate-Reducing Bacterium Isolated from Deep-Sea Hydrothermal Vents of the Mid-Atlantic Ridge
Authors: Abin Christopher A. , Hollibaugh James T. 
Published: November 2016

ABSTRACT

Vulcanibacillus modesticaldus
BR
T
was isolated from calcite-rich, metalliferous core samples collected at the Rainbow deep-sea hydrothermal vent field on the Mid-Atlantic Ridge. Here, we report the 2.2-Mb draft genome sequence for this strain, consisting of 100 contigs with a G+C content of 33.6% and 2,227 protein-coding sequences.
Complete Genome Sequences of the Neethling-Like Lumpy Skin Disease Virus Strains Obtained Directly from Three Commercial Live Attenuated Vaccines
Authors: Mathijs Elisabeth , Vandenbussche Frank , Haegeman Andy , King Alasdair , Nthangeni Bethuel , Potgieter Christiaan , Maartens Louis , Van Borm Steven , De Clercq Kris 
Published: November 2016

ABSTRACT
Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete genome sequences of three LSDV strains obtained directly from the live attenuated vaccines: Lumpyvax (MSD Animal Health), Herbivac LS (Deltamune) and Lumpy Skin Disease Vaccine (Onderstepoort Biological Products).
Draft Genome Sequences of Two Marinitoga camini Isolates Producing Bacterioviruses
Authors: Mercier Coraline , Lossouarn Julien , Haverkamp Thomas , Bienvenu Nadège , Godfroy Anne , Cueff-Gauchard Valérie , Geslin Claire , Nesbo Camilla 
Published: November 2016

ABSTRACT

Here, we present the draft genome sequences of two thermophilic
Marinitoga
strain members of the
Thermotogales
order,
Marinitoga camini
DV1155 and
Marinitoga camini
DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge.
Finished Genome Sequences of Xanthomonas fragariae, the Cause of Bacterial Angular Leaf Spot of Strawberry
Authors: Henry Peter M. , Leveau Johan H. J. 
Published: November 2016

ABSTRACT

Xanthomonas fragariae
is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit. Long-read sequencing was employed to generate finished genomes for two isolates (each with one chromosome and two plasmids) from symptomatic plants in northern California.
First Draft Genome Sequences of
Neisseria
sp. Strain 83E34 and
Neisseria
sp. Strain 74A18
,
Previously Identified as CDC Eugonic Fermenter 4b Species
Authors: Greninger Alexander L. , Streithorst Jessica , Chiu Charles Y. , Miller Steve 
Published: November 2016

ABSTRACT

We report the first draft genome sequences of two isolates previously classified as CDC EF-4b species,
Neisseria
sp. 83E34 and
Neisseria
sp. 74A18. Both strains were isolated from patients with animal bites and likely constitute novel genomospecies with average nucleotide identities of <95% to other sequenced strains.
Missed opportunities for earlier diagnosis of HIV in patients who presented with advanced HIV disease: a retrospective cohort study
Authors: Levy Itzchak , Maor Yasmin , Mahroum Naim , Olmer Liraz , Wieder Anat , Litchevski Vladislav , Mor Orna , Rahav Galia 
Published: November 2016

ObjectiveTo quantify and characterise missed opportunities for earlier HIV diagnosis in patients diagnosed with advanced HIV.DesignA retrospective observational cohort study.SettingA central tertiary medical centre in Israel.MeasuresThe proportion of patients with advanced HIV, the proportion of missed opportunities to diagnose them earlier, and the rate of clinical indicator diseases (CIDs) in those patients.ResultsBetween 2010 and 2015, 356 patients were diagnosed with HIV, 118 (33.4%) were diagnosed late, 57 (16%) with advanced HIV disease. Old age (OR=1.45 (95% CI 1.16 to 1.74)) and being heterosexual (OR=2.65 (95% CI 1.21 to 5.78)) were significant risk factors for being diagnosed late. All patients with advanced disease had at least one CID that did not lead to an HIV test in the 5 years prior to AIDS diagnosis. The median time between CID and AIDS diagnosis was 24 months (IQR 10–30). 60% of CIDs were missed by a general practitioner and 40% by a specialist.ConclusionsMissed opportunities to early diagnosis of HIV occur in primary and secondary care. Lack of national guidelines, lack of knowledge regarding CIDs and communication barriers with patients may contribute to a late diagnosis of HIV.
Survey of community pharmacists' perception of electronic cigarettes in London
Authors: Marques Gomes Ana C N , Nabhani-Gebara Shereen , Kayyali Reem , Buonocore Federico , Calabrese Gianpiero 
Published: November 2016

ObjectivesTo seek community pharmacists' perception on use, safety and possible effectiveness of e-cigarettes as quit smoking tools, and their future regulation.SettingA survey of a sample of 154 community pharmacies across London, UK.ContextE-cigarettes have exclusively established themselves in the market through consumers-led demand. To date, e-cigarettes still remain unregulated and can be easily purchased in shops, over the internet, but more controversially also in pharmacies in the UK. Pharmacists find themselves with a shortage of information on their safety and efficacy, and may experience an ethical dilemma when consulted by patients/customers.Key findingsResponse rate: 60% (n=92). Independent pharmacies accounted for 90% of the sample. The majority of participants (73%) sell e-cigarettes. A minority of participants (20%) have been presented with adverse effects such as cough and dry mouth. As possible reasons for their use, pharmacists ranked ‘aid in stop smoking’ as the most important (56%), with ‘cheaper alternative’ (43%) and ‘social/recreational use’ (31%) being the least important ones. Safety issues were raised as statements such as ‘e-liquid in cartridges may be toxic’ were agreed by 52% of respondents. The majority of pharmacists (97%) were supportive of e-cigarettes being regulated, expressing current concerns regarding excipients (42%) and nicotine content (34%). Participants indicated that they would require training in the form of information packs (88%), online tutorials (67%), continuous professional development (CPD) workshops (43%) to cover safety, counselling, dosage instructions, adverse effects and role in the smoking cessation care pathway in the future.ConclusionsPharmacists expressed concerns about the safety of e-cigarettes, especially regarding the amounts of excipients and nicotine as these still remain unregulated. Currently, there are no guidelines for pharmacists regarding e-cigarettes. Community pharmacists look forward to regulations so to conduct their duties in a more confident and legislated fashion.
Improving pneumococcal vaccination rates of medical inpatients in urban Nepal using quality improvement measures
Authors: Bock Allison , Chintamaneni Kathan , Rein Lisa , Frazer Tifany , Kayastha Gyan , MacKinney Theodore 
Published: November 2016

Streptococcus pneumoniae infection is associated with high morbidity and mortality in low income countries. In Nepal, there is a high lung disease burden and incidence of pneumonia due to multiple factors including indoor air pollution, dust exposure, recurrent infections, and cigarette smoking. Despite the ready availability of effective pneumococcal vaccines (PNV), vaccine coverage rates remain suboptimal globally. Quality Improvement (QI) principles could be applied to improve compliance, but it is a virtually new technology in Nepal.This QI study for Patan Hospital sought to introduce the concept of QI there, to measure the baseline pneumococcal vaccination rate of qualifying adult patients discharged from the medical wards and to assess reasons for non-vaccination. QI interventions were instituted to improve this rate, measuring the effectiveness of QI methods to produce the desired outcomes using the Model for Improvement, Plan-Do-Study-Change (PDSA) methodology.In the three week baseline assessment, 2 out of 81 (2%) eligible patients recalled ever receiving a prior pneumococcal vaccine; 68 (84%) unvaccinated patients responded that they were not asked or were unaware of the PNV. After the QI interventions, the pneumococcal vaccination rate significantly increased to 42% (23/56, p<0.001). Post-intervention, the leading reason for non-vaccination was cost (20%, 11/56). Only 5 (9%) unvaccinated patients were not asked or were unaware of the PNV, a significant change in that process outcome from baseline (p<0.001).Quality improvement measures were effective in increasing pneumococcal vaccination rates, despite the limited familiarity with QI methods at this major teaching hospital. QI techniques may be useful in this and other efforts to improve quality in resource-limited settings, without great cost.
Be(ing) prepared: Guide and Scout participation, childhood social position and mental health at age 50—a prospective birth cohort study
Authors: Dibben Chris , Playford Chris , Mitchell Richard 
Published: November 2016

BackgroundMental health is a major concern in many countries. We explore whether youth participation in the Scouts and Guides could protect mental health in later life and in particular whether it might reduce inequalities in mental health associated with early life socioeconomic position.MethodsUsing the 1958 birth cohort National Child Development Study, we tested whether Scouts–Guide attendance was associated with mental health (SF-36, Mental Health Index (MHI-5)) controlling for childhood risk factors and interacted with social class.ResultsOf the 9603 cohort members, 28% had participated in the Scouts–Guides. The average MHI-5 score was 74.8 (SD 18.2) at age 50. After adjustment, for potential childhood confounders, participation in Scouts–Guides was associated with a better MHI-5 score of 2.22 (CI 1.32 to 3.08). Among those who had not been a Scout–Guide, there was a gradient in mental health at age 50 by childhood social position, adjusting for other childhood risk factors. This gradient was absent among those who had been a Scout–Guide. Scout–Guides had an 18% lower odds of an MHI-5 score indicative of mood or anxiety disorder. The findings appeared robust to various tests for residual confounding.ConclusionsParticipation in Guides or Scouts was associated with better mental health and narrower mental health inequalities, at age 50. This suggests that youth programmes that support resilience and social mobility through developing the potential for continued progressive self-education, ‘soft’ non-cognitive skills, self-reliance, collaboration and activities in natural environments may be protective of mental health in adulthood.
Simple mass estimates for resonance(s) being 6 top + 6 antitop bound states and combinations thereof
Authors: Nielsen H. B. 
Published: November 2016

We have long speculated,[Formula: see text] that 6 top + 6 antitop quarks due to the relatively large size of the top-Yukawa coupling would bind exceptionally strongly by mainly Higgs exchange. Here we present a surprisingly simple “calculation” of the mass of this speculated bound state. Even a possible resonance in scattering of two such bound states is speculated. For the “calculation” of the masses it is crucial to assume, that our since long speculated principle “multiple point principle,”[Formula: see text] is true. This principle says: there are several vacua all having almost zero energy density. Further, we make an approximation of the Higgs Yukawa potential essentially replacing the exponential in it by a step-function. The new result means that there are now two independent calls for our bound state having the mass around 750 GeV required by our “new law of nature” the Multiple Point Principle. It should be remarked that in our picture there is no new physics in the sense of new fundamental particles, but the “multiple point principle” is new in the sense of being not yet accepted. Further, we get the same mass within uncertainties as earlier2 but now from a completely different assumption, except for being from our “multiple point principle.” But the two masses are gotten from using different (speculative) vacua occurring in the pure Standard Model.
Abstract A01: Trichodermin induces G1/S cell cycle arrest in ovarian cancer cells
Authors: Chen Yi C. , Gao Ying 
Published: November 2016

Abstract
Ovarian cancer is a major health concern for women as it is prominent among gynecologic cancers. Trichodermin is a sesquiterpene isolated from Nalanthamala psidii and found to induce apoptosis in human chondrosarcoma cells. In this study, A2780/CP70 and OVCAR-3 ovarian cancer cell lines were treated with trichodermin to determine whether it had an anti-cancer effect. Our data presented that trichodermin reduced the viability of both ovarian cancer cells by inducing G1/S cell cycle arrest rather than apoptosis. The underlying mechanism might be that trichodermin down-regulated the expression of Cyclin D1 via inhibiting AhR and c-Myc expression. Therefore, trichodermin is promising in ovarian cancer treatment.
Citation Format: Yi C. Chen, Ying Gao. Trichodermin induces G1/S cell cycle arrest in ovarian cancer cells. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Therapeutic Response; Feb 28-Mar 2, 2016; Orlando, FL. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(11_Suppl):Abstract nr A01.
Abstract A08: Mechanistic basis of Palbociclib combinatorial activity in ER+ breast cancer and non-breast indications
Authors: Dann Stephen , Chionis John , Choating Liu , Chen Enhong , Wei Ping , Eisele Koleen , Shields David J. , Rejto Paul A. , VanArsdale Todd 
Published: November 2016

Abstract
Phosphorylation of the retinoblastoma protein (Rb) by cyclin-dependent kinases 4 and 6 (CDK4/6) is a critical checkpoint for G1/S cell cycle progression and commitment to cellular proliferation. Human malignancies often subvert these control mechanisms through a range of genetic and biochemical adaptations. Accordingly, tumors that depend on CDK4/6 activity for proliferation and survival are particularly sensitive to inhibition of this pathway by palbociclib (Ibrance™), a highly selective inhibitor of CDK4/6 kinase activities. Treatment regimen of palbociclib with letrozole significantly improved progression-free survival in a randomized phase 2 study of women with advanced estrogen receptor-positive (ER+), HER2-negative breast cancer. Likewise, in ER+ breast cancer models palbociclib and estrogen antagonists combine for greater anti-proliferative activity, increased hallmarks of cellular senescence and prolonged durability of response following drug removal. Dual inhibition of CDK4/6 and ER signaling demonstrated robust anti-tumor activity in xenograft studies. The addition of Palbociclib to other targeted therapeutics elicits improved activity in pre-clinical models of several non-breast indications and these effects also manifest through modulation of cellular proliferation, senescence and growth arrest. Data will be presented on the molecular basis of combination benefit with Palbociclib in ER+ breast and other oncology indications.
Citation Format: Stephen Dann, John Chionis, Liu Choating, Enhong Chen, Ping Wei, Koleen Eisele, David J. Shields, Paul A. Rejto, Todd VanArsdale. Mechanistic basis of Palbociclib combinatorial activity in ER+ breast cancer and non-breast indications. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Therapeutic Response; Feb 28-Mar 2, 2016; Orlando, FL. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(11_Suppl):Abstract nr A08.