bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Knowledge of Research on Rehearsal Skills and Teaching Values of Experienced Teachers
Authors: Yarhrough Cornelia , Price Harry E. , Bowers Judy 
Published: January 2017

The following article, although a bit unusual is pertinent to this journal. It is not a review of research literature, nor is it a study about a single aspect of teaching music. It is, rather, a study about the application of research knowledge to teaching skills and values by experienced teachers. Update is based on the idea that if teachers are informed about the results of research, they will apply these results to their teaching. We all hope that this is true, and the results of this study seem to support the notion. Indeed, in this study, the researchers systematically examined how teachers' attitudes and skills changed as a result of being informed about research in a specific area: sequential patterns of instruction. The teachers chose to change without any contingencies operating to encourage or discourage this change. Work in understanding the sequential patterns of instruction in musk: is based on an ongoing line of research It is apparent that many music teachers and ensemble directors believe this concept is relevant, comprehensible, and easily applied.-H.E.P.
Functional role and therapeutic targeting of p21-activated kinase 4 in multiple myeloma
Authors: Fulciniti Mariateresa , Martinez-Lopez Joaquin , Senapedis William , Oliva Stefania , Lakshmi Bandi Rajya , Amodio Nicola , Xu Yan , Szalat Raphael , Gulla Annamaria , Samur Mehmet K. , Roccaro Aldo , Linares Maria , Cea Michele , Baloglu Erkan , Argueta Christian , Landesman Yosef , Shacham Sharon , Liu Siyuan , Schenone Monica , Wu Shiaw-Lin , Karger Barry , Prabhala Rao , Anderson Kenneth C. , Munshi Nikhil C. 
Published: January 2017

Key Points
High expression of PAK4 promotes myeloma cell proliferation through activation of MM antiapoptotic and survival pathways. Targeting PAK4 with a novel small molecule inhibitor, KPT-9274, has significant impact on MM cell growth and survival.
How I treat acquired aplastic anemia
Authors: Bacigalupo Andrea 
Published: January 2017

Abstract
Acquired severe aplastic anemia (SAA) is a rare hematologic disease associated with significant morbidity and mortality. Immune destruction of hemopoietic stem cells plays an important role in pathogenesis, as shown by successful treatment with immunosuppressive agents, leading to transfusion independence or complete recovery of peripheral blood counts in a proportion of patients. Growth factors can be combined with immunosuppressive therapy (IST) and may improve response rates, as recently shown with thrombopoietin analogs. Anabolic steroids may still play a role in combination with IST. The problem with IST is failure to respond and the development of late clonal disorders. Bone marrow transplantation (BMT) is the other therapeutic option: a matched sibling donor remains the best choice. For patients lacking a matched family donor, unrelated donors can be readily found, although mostly for patients of Caucasian origin. Other BMT options include unrelated cord blood or mismatched family donors. Acute and chronic graft-versus-host disease remain important complications of BMT. Patient age is a strong predictor of outcome for both IST and BMT, and must be considered when designing therapeutic strategies. Early diagnosis and treatment, as well as long-term monitoring, remain crucial steps for successful treatment of SAA.
Platelets activate a pathogenic response to blood-stage Plasmodium infection but not a protective immune response
Authors: Gramaglia Irene , Velez Joyce , Combes Valery , Grau Georges E. R. , Wree Melanie , van der Heyde Henri C. 
Published: January 2017

Key PointsPlatelets are not killer cells of blood-stage Plasmodium parasites. Platelets are not required to activate the protective immune response to blood-stage Plasmodium infection in mice.
The antineoplastic drug, trastuzumab, dysregulates metabolism in iPSC‐derived cardiomyocytes
Authors: Necela Brian M. , Axenfeld Bianca C. , Serie Daniel J. , Kachergus Jennifer M. , Perez Edith A. , Thompson E. Aubrey , Norton Nadine 
Published: January 2017

AbstractBackgroundThe targeted ERBB2 therapy, trastuzumab, has had a tremendous impact on management of patients with HER2+ breast cancer, leading to development and increased use of further HER2 targeted therapies. The major clinical side effect is cardiotoxicity but the mechanism is largely unknown. On the basis that gene expression is known to be altered in multiple models of heart failure, we examined differential gene expression of iPSC‐derived cardiomyocytes treated at day 11 with the ERBB2 targeted monoclonal antibody, trastuzumab for 48 h and the small molecule tyrosine kinase inhibitor of EGFR and ERBB2.ResultsTranscriptome sequencing was performed on four replicates from each group (48 h untreated, 48 h trastuzumab and 48 h lapatinib) and differential gene expression analyses were performed on each treatment group relative to untreated cardiomyocytes. 517 and 1358 genes were differentially expressed, p < 0.05, respectively in cardiomyocytes treated with trastuzumab and lapatinib. Gene ontology analyses revealed in cardiomyocytes treated with trastuzumab, significant down‐regulation of genes involved in small molecule metabolism (p = 3.22 × 10−9) and cholesterol (p = 0.01) and sterol (p = 0.03) processing. We next measured glucose uptake and lactate production in iPSC‐derived cardiomyocytes 13 days post‐plating, treated with trastuzumab up to 96 h. We observed significantly decreased glucose uptake from the media of iPSC‐derived cardiomyocytes treated with trastuzumab as early as 24 h (p = 0.001) and consistently up to 96 h (p = 0.03).ConclusionsOur study suggests dysregulation of cardiac gene expression and metabolism as key elements of ERBB2 signaling that could potentially be early biomarkers of cardiotoxicity.
Increased Perinatal Morbidity and Mortality Among Asian American and Pacific Islander Women in the United States
Authors: Siddiqui Maryam , Minhaj Mohammed , Mueller Ariel , Tung Avery , Scavone Barbara , Rana Sarosh , Shahul Sajid 
Published: January 2017

BACKGROUND:
Asian American/Pacific Islanders (AAPIs) are the fastest-growing racial group in the United States. Despite a higher socioeconomic status, AAPI women experience higher rates of maternal morbidity and mortality.

METHODS:
Using the National Inpatient Sample, we performed a retrospective cohort analysis of women who were hospitalized for delivery from 2002 to 2013. The primary outcome variable was inpatient mortality rate, and the presence of severe maternal morbidities was estimated using the Bateman Comorbidity Index, a validated tool for predicting obstetric morbidity.

RESULTS:
AAPI women presenting for delivery between 2003 and 2012 were older, more likely to reside in a zip code in the top quartile of annual income, be privately insured than Caucasian women, and less likely to have a higher Bateman Comorbidity Index. However, AAPI women had a higher likelihood of postpartum hemorrhage (3.4% vs 2.7%, P < .001), uterine atony, severe perineal lacerations, and severe maternal morbidities. Procedures such as transfusion, hysterectomy, and mechanical ventilation were also more common in AAPI women. Furthermore, AAPI women had a higher mortality rate that persisted despite adjustment for an apparently higher income and comorbidities (odds ratio 1.72, 95% confidence interval: 1.14–2.59, P = .01).

CONCLUSIONS:
Despite having a higher socioeconomic status, AAPI women had higher rates of maternal mortality during hospitalization for delivery. This increase persisted even after adjustment for factors known to affect peripartum outcomes. Further investigation is needed to better clarify the causes of racial differences in maternal morbidity and mortality.
Anesthetic Outcomes of Children With Arthrogryposis Syndromes: No Evidence of Hyperthermia
Authors: Gleich Stephen J. , Tien Michael , Schroeder Darrell R. , Hanson Andrew C. , Flick Randall , Nemergut Michael E. 
Published: January 2017

BACKGROUND:
Arthrogryposis syndromes are a heterogeneous group of disorders characterized by congenital joint contractures often requiring multiple surgeries during childhood to address skeletal and visceral abnormalities. Previous reports suggest that these children have increased perioperative risk, including hypermetabolic events discrete from malignant hyperthermia, difficult airway management, isolated hyperthermia, and difficult IV line placement. We sought to compare children with arthrogryposis multiplex congenita (AMC) versus the less severe, distal arthrogryposis syndromes (DAS) and to evaluate possible intraoperative hyperthermia of patients with AMC. We hypothesized that children with AMC had a greater incidence of intraoperative hyperthermia and more difficulty with airway management and IV access.

METHODS:
Children aged 0 to 25 years with arthrogryposis syndromes who underwent anesthesia from 1972 to 2013 were identified. The medical records were reviewed for demographics, arthrogryposis type, and anesthetic complications. AMC subjects were compared with DAS subjects. To evaluate the probability of hyperthermia and hypermetabolic responses of patients with AMC, we performed a post hoc case–control analysis. Patients with AMC were matched in a 1:2 ratio to patients without arthrogryposis to evaluate the primary outcome of maximum intraoperative temperature.

RESULTS:
Forty-five patients with AMC and 16 patients with DAS underwent 264 and 105 unique anesthetics, respectively. There was no significant difference in intraoperative hyperthermia or hypermetabolic events (odds ratio [OR], 0.94; 95% confidence interval [CI], 0.36–2.47; P = .90). Children with AMC were more likely to have difficult IV access (OR, 7.1; 95% CI, 1.81–27.90; P = .005). Additional evidence suggested that difficult airway management (OR, 4.06; 95% CI, 1.01–16.39; P = .049) and hemodynamic instability (OR, 4.22; 95% CI, 1.03–17.26; P = .045) were more likely in children with AMC. From post hoc case–control analysis, there was no significant difference in the mean maximum intraoperative temperature (estimated difference +0.04°C; 95% CI, −0.14 to +0.22; P = .64) or odds of intraoperative hyperthermia (OR, 1.49; 95% CI, 0.78–2.82; P = .223) for patients with AMC compared with control subjects.

CONCLUSIONS:
Children with arthrogryposis syndromes present challenges to the anesthesia and surgical teams, including greater neuromuscular disease burden and challenging peripheral IV placement, with additional evidence suggesting difficult airway management and intraoperative hemodynamic instability. Although more definitive studies are warranted, we did not find evidence of increased odds of intraoperative hyperthermia or hypermetabolic responses.
CASZ1 loss-of-function mutation contributes to familial dilated cardiomyopathy
Authors: Qiu Xing-Biao , Qu Xin-Kai , Li Ruo-Gu , Liu Hua , Xu Ying-Jia , Zhang Min , Shi Hong-Yu , Hou Xu-Min , Liu Xu , Yuan Fang , Sun Yu-Min , Wang Jun , Huang Ri-Tai , Xue Song , Yang Yi-Qing 
Published: January 2017

AbstractBackground:The zinc finger transcription factor CASZ1 plays a key role in cardiac development and postnatal adaptation, and in mice, deletion of theMethods:The coding exons and splicing junction sites of theResults:A novel heterozygous CASZ1 mutation, p.K351X, was identified in an index patient with DCM. Genetic analysis of the mutation carrier’s family showed that the mutation co-segregated with DCM, which was transmitted in an autosomal dominant pattern with complete penetrance. The nonsense mutation, which was absent in 400 referential chromosomes, altered the amino acid that was highly conserved evolutionarily. Biological investigations revealed that the mutant CASZ1 had no transcriptional activity.Conclusions:The current study reveals
Assessment of autoantibodies to interferon-ω in patients with autoimmune polyendocrine syndrome type 1: using a new immunoprecipitation assay
Authors: Larosa Maria del Pilar , Mackenzie Rachel , Burne Peter , Garelli Silvia , Barollo Susi , Masiero Stefano , Rubin Beatrice , Chen Shu , Furmaniak Jadwiga , Betterle Corrado , Smith Bernard Rees 
Published: January 2017

AbstractBackground:Measurements of autoantibodies to interferon-ω (IFN-ω) in patients with autoimmune polyglandular syndrome type 1 (APS-1) were performed using a new immunoprecipitation assay (IPA) based onMethods:We have developed and validated a new IPA based onResults:Sixty-six (84.6%) of 78 APS-1 patients were positive for IFN-ω Ab usingConclusions:We have developed a novel, highly sensitive and specific assay for measurement of IFN-ω Ab. It provides a simple and convenient method for the assessment of patients with APS-1 and selecting patients suspected of having APS-1 for
The clinical significance of platelet microparticle-associated microRNAs
Authors: Provost Patrick 
Published: January 2017

AbstractCirculating blood platelets play a central role in the maintenance of hemostasis. They adhere to subendothelial extracellular matrix proteins that become exposed upon vessel wall damage, which is followed by platelet activation, further platelet recruitment, platelet aggregation and formation of an occlusive, or non-occlusive, platelet thrombus. Platelets host a surprisingly diverse transcriptome, which is comprised of ~9500 messenger RNAs (mRNAs) and different classes of non-coding RNAs, including microRNAs, as well as a significant repertoire of proteins that contribute to their primary (adhesion, aggregation, granule secretion) and alternative (RNA transfer, mRNA translation, immune regulation) functions. Platelets have the propensity to release microparticles (MPs; 0.1–1 μm in diameter) upon activation, which may mediate inflammatory responses and contribute to exacerbate inflammatory diseases and conditions. Carrying components of the platelets’ cytoplasm, platelet MPs may exert their effects on recipient cells by transferring their content in platelet-derived bioactive lipid mediators, cytokines, mRNAs and microRNAs. Platelet MP-associated microRNAs may thus function also outside of platelets and play an important role in intercellular signaling and gene expression programming across the entire circulatory system. The role and importance of platelet MP-associated microRNAs in various aspects of biology and pathophysiology are increasingly recognized, and now provide the scientific basis and rationale to support further translational research and clinical studies. The clinical significance, pathophysiological role as well as the diagnostic and therapeutic potential of platelet MP-associated microRNAs in cardiovascular diseases, platelet transfusion and cancer will be discussed.
Recalibrating the Spatiality of the State: The Normality of Abnormal Transgressions in the Third World
Authors: Menon Parvathi 
Published: January 2017

AbstractThe paper argues that the opposing ideas of order/disorder, peace/war and normality/abnormality exist within each other, making a discernible boundary between them a fallacy created by the language of law. Therefore, even when a resistance to the order is carried out, it is with the aspiration of assimilation into this phantom world community. I analyze how the concept of nation-state is reinforced through territorial identities, best portrayed through liberation struggles, thus demonstrating how these transgressions, though projected by the international order to exist separate from the order (normality) as an ‘abnormality’, is in fact facilitated through (the normality of) law.
Experimental optimization of microwave drying zinc oxide leach residues by response surface methodology
Authors: Zhang Weifeng , Chen Junruo , Lin Guo , Zhang Libo 
Published: January 2017

Abstract
Response surface methodology (RSM) and Box-Behnken design (BBD) were applied to experiment optimization of microwave drying zinc oxide leach residues (ZOLR). The effects of different factors involving temperature, drying time, material mass, material thickness and their interaction on moisture content and dehydration rate of ZOLR were investigated. Two second-order polynomial models for moisture content and dehydration rate of ZOLR were established by multivariate regression analysis. Analysis of variance shows that all independent variable and quadratic terms of the four factors have significant influence on moisture content and dehydration rate of ZOLR. The optimum drying conditions are as follows: temperature is 93.41°C, drying time is 15.9 min, sample mass is 77.91 g and material thickness is 17 mm. The predicted values of moisture content and dehydration rate were 10.98% and 1 g/min, which were close to experimental values 11±0.03% and 1±0.01 g/min under the optimal conditions. Infrared spectra analysis show that no chemical reactions took place in the process of microwave dying ZOLR and only a part of the absorbed water was removed in the sample.
Synthesis of 1,4-dihydropyrimidines with immobilized urease: effect of method immobilization on magnetic supports
Authors: Vargas Annie Y. , Rojas Hugo A. , Romanelli Gustavo P. , Martínez José J. 
Published: January 2017

AbstractThe effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe
Bone health assessment of food allergic children on restrictive diets: a practical guide
Authors: Doulgeraki Artemis E. , Manousakis Emmanouel M. , Papadopoulos Nikolaos G. 
Published: January 2017

AbstractBackground:Food allergy in childhood is on the rise globally and is managed with avoidance diets; recent case reports of food allergic children with nutritional rickets in the literature highlight the importance of close monitoring of bone health in this population.Methods:There is no consensus as yet with regard to bone health evaluation in food allergic children; therefore, extensive literature search was performed and the existing evidence is presented, along with a relevant algorithm.Results:Children allergic to cow’s milk protein or presenting with allergy in more than three food items, as well as patients with severe allergic phenotypes or comorbidities known to affect the skeleton, seem to be at risk of metabolic bone disorders. As a practical guide, suspicious cases can be investigated with basic bone profile, whereas more severe cases (persistent bone pain and fractures) may undergo advanced bone health assessment, with bone mineral density (BMD) and metabolic bone markers’ evaluation. Of note, these diagnostic steps call for further studies in the field of food allergy, as they are not performed as a routine. Evidence is accumulating with regard to vitamin D deficiency, osteopenia and imbalanced bone metabolism in those food allergic children who show poor dietary compliance or have inadequate medical supervision.Conclusions:Ensuring optimal bone accrual in a food allergic child is an important task for the clinician and requires close monitoring of the restrictive diet and prompt therapeutic intervention, in an effort to avoid rickets or osteopenia.
Epidermal growth factor receptor (EGFR) involvement in successful growth hormone (GH) signaling in GH transduction defect
Authors: Kostopoulou Eirini , Rojas-Gil Andrea Paola , Karvela Alexia , Spiliotis Bessie E. 
Published: January 2017

AbstractBackground:Growth hormone (GH) transduction defect (GHTD) is a growth disorder with impaired signal transducer and activator of transcription 3 (STAT3) phosphorylation mediated by overexpression of cytokine-inducible SH2-containing protein (CIS), which causes increased growth hormone receptor (GHR) degradation. This study investigated the role of epidermal growth factor (EGF) in the restoration of normal GH signaling in GHTD.Methods:Protein expression, cellular localization and physical contact of proteins of the GH and EGF signaling pathways were studied by Western immunoblotting, immunofluorescence and co-immunoprecipitation, respectively. These were performed in fibroblasts of one GHTD patient (P) and one control child (C) at the basal state and after induction with human GH (hGH) 200 μg/L (GH200), either with or without silencing of CIS mRNA, and after induction with hGH 1000 μg/L (GH1000) or 50 ng/mL EGF.Results:The membrane availability of the EGF receptor (EGFR) and the activated EGFR (pEGFR) was increased in P only after simultaneous GH200 and silencing of CIS mRNA or with GH1000, whereas this occurred in C after GH200 alone. After EGF induction, the membrane localization of GHR, STAT3 and that of EGFR were increased in P more than in C.Conclusions:In conclusion, in GHTD, the EGFR seems to participate in successful GH signaling, but induction of GHTD fibroblasts with a higher dose of hGH is needed. The EGF/EGFR pathway, in contrast to the GH/GHR pathway, seems to function normally in P and is more primed compared to C. The involvement of the EGFR in successful GH signaling may explain the catch-up growth seen in the Ps when exogenous hGH is administered.
Efficacy of long-term growth hormone therapy in short non-growth hormone-deficient children
Authors: Schena Lucia , Meazza Cristina , Pagani Sara , Paganelli Valeria , Bozzola Elena , Tinelli Carmine , Buzi Fabio , Bozzola Mauro 
Published: January 2017

AbstractBackground:In recent years, several studies have been published showing different responses to growth hormone (GH) treatment in idiopathic short stature children. The aim of the present study was to investigate whether non-growth-hormone-deficient (non-GHD) short children could benefit from long-term GH treatment as GHD patients.Methods:We enrolled 22 prepubertal children and 22 age- and sex-matched GHD patients, with comparable height, body mass index (BMI), bone age, and insulin-like growth factor 1 (IGF-I) circulating levels. The patients were treated with recombinant human GH (rhGH) and followed until they reach adult height.Results:During GH treatment, the two groups grew in parallel, reaching the same final height-standard deviation score (SDS) and the same height gain. On the contrary, we found significantly lower IGF-I serum concentrations in non-GHD patients than in GHD ones, at the end of therapy (p=0.0055).Conclusions:In our study, the response to GH treatment in short non-GHD patients proved to be similar to that in GHD ones. However, a careful selection of short non-GHD children to be treated with GH would better justify the cost of long-term GH therapy.