bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanoscale Distribution of Transforming Growth Factor Receptor on Post‐Golgi Vesicle Revealed by Super‐resolution Microscopy
Authors: Ruan Hefei , Yu Jianqiang , Yuan Jinghe , Li Nan , Fang Xiaohong 
Published: October 2016

AbstractTransforming growth factor‐β (TGF‐β) type II receptor (TβRII) plays a critical role in the initiation of TGF‐β signaling pathway; therefore, the study of its synthesis and transport processes is of great important. In this work, we achieved super‐resolution imaging of a new type of TβRII‐containing post‐Golgi vesicle by our home‐built stimulated emission depletion (STED) microscope. We visualized the ring‐shaped structure of these vesicles containing newly synthesized TβRII in the cytoplasm and characterized their size distribution from 300 to 1000 nm. These vesicles could be swollen by chloroquine treatment. Further investigation revealed that TβRII formed clusters on the outer ring of the post‐Golgi vesicles. This study offers new information on the intracellular transportation of TGF‐β receptors for better understanding its signaling process.
Particle Smoother for Nonlinear Systems With One‐Step Randomly Delayed Measurements
Authors: Yu‐Long Huang , Yong‐Gang Zhang 
Published: October 2016

AbstractIn this paper, a new particle smoother based on forward filtering backward simulation is developed to solve the nonlinear and non‐Gaussian smoothing problem when measurements are randomly delayed by one sampling time. The heart of the proposed particle smoother is computation of delayed posterior filtering density based on stochastic sampling approach, whose particles and corresponding weights are updated in Bayesian estimation framework by considering the one‐step randomly delayed measurement model. The superior performance of the proposed particle smoother as compared with existing methods is illustrated in a numerical example concerning univariate non‐stationary growth model.
Analysis of incoming biomass burning aerosol plumes over southern Brazil
Authors: de Oliveira Aline Macedo , Mariano Glauber Lopes , Alonso Marcelo Félix , Mariano Ericka Voss Chagas 
Published: October 2016

AbstractWe characterized the aerosol particles and their transport to southern Brazil during the austral winter months of June, July, and August from 2002 to 2011. To obtain the aerosol optical depth (AOD) (0.55 μm) and the Ångström exponent (0.47–0.66 μm), we used MODIS data. High values of AOD were observed in 2002, 2005, 2007, and 2010, with the highest values in August. We also used the CCATT–BRAMS model to simulate the AOD in southern Brazil. Both approaches suggest an increase of the AOD in the northwestern part of the region, evidence for the transport of biomass burning plumes from central Brazil.
Optimal design of a short‐turning strategy considering seat availability
Authors: Ji Yuxiong , Yang Xiyu , Du Yuchuan 
Published: October 2016

SummaryWe develop a methodology to optimize the schedule coordination of a full‐stop service pattern and a short‐turning service pattern on a bus route. To capture the influence of bus crowding and seat availability on passengers' riding experience, we develop a Markov model to describe the seat‐searching process of a passenger and an approach to estimate the transition probabilities of the Markov model. An optimization model that incorporates the Markov model is proposed to design the short‐turning strategy. The proposed model minimizes the total cost, which includes operational cost, passengers' waiting time cost and passengers' in‐vehicle travel time cost. Algorithm is developed to produce optimal values of the decision variables. The proposed methodology is evaluated in a case study. Compared with methodologies that ignore the effect of bus crowding, the proposed methodology could better balance bus load along the route and between two service patterns, provide passengers with better riding experience and reduce the total cost. In addition, it is shown that the optimal design of the short‐turning strategy is sensitive to seat capacity. Copyright © 2016 John Wiley & Sons, Ltd.
Monitoring der neuen Schrägseilbrücke über den Chao Phraya, Nonthaburi, Thailand
Authors: Mack Thomas , Geier Roman , Kahl Thomas , Kasper Marcel 
Published: October 2016

AbstractIm Norden von Bangkok (Thailand) wurde in den letzten Jahren zur Entlastung der umliegenden Straßenzüge in der Provinz Nonthaburi ein neuer Autobahnabschnitt realisiert, dessen Hauptmerkmal eine Extradosed‐Brücke über den Chao Phraya mit einer Gesamtlänge von 460 m darstellt. Das Bauwerk, bestehend aus zwei Pylonen mit goldener Kuppel, trägt mit 96 Schrägseilen einen Hohlkastenquerschnitt, ausgelegt für sechs Fahrstreifen, über den Fluss. Zur Überwachung des Tragverhaltens wurde vom Bauherrn ein umfangreiches Monitoringsystem ausgeschrieben, welches von Schimetta Consult in Kooperation mit DYWIDAG Systems International GmbH optimiert, geplant und umgesetzt wurde. 45 Sensoren überwachen dauerhaft die Temperaturen, Dehnungen und Durchbiegungen der Brücke, Neigungen der Pylone, Öffnung der Fahrbahnübergänge, Windgeschwindigkeiten, Beschleunigungen und Seilkräfte. Die Daten werden automatisiert vor Ort gespeichert, über eine UMTS‐Verbindung auf einen externen Server übermittelt und dem Bauherrn innerhalb weniger min über eine Homepage zur laufenden Kontrolle zur Verfügung gestellt. Darüber hinaus werden die Messdaten in einem halbjährlichen Intervall zusammengefasst und analysiert sowie die Ergebnisse dem Bauherrn in einem Messbericht übermittelt. Das Monitoringsystem zeichnet seit Messbeginn Anfang 2015 zuverlässig Daten auf und ermöglicht so einen guten Einblick in das Tragverhalten des Objekts.
Feuchtemessungen in Bauteilen und anderen Strukturen mit Bluetooth Low Energy
Authors: Köppe Enrico , Moldenhauer Laura , Haamkens Frank , Helmerich Rosemarie 
Published: October 2016

AbstractDie Langzeitüberwachung von Bauteilen (Structural Health Monitoring, SHM) ging in den letzten Jahren zunehmend von verkabelten Sensoren [1] zu kabellosen Sensornetzwerken (WSN) [2] über. Frühwarnsysteme sollen rechtzeitige Signale senden, wenn Schädigungsprozesse einsetzen, wenn ein Bauteil z. B. zu feucht wird (Deiche, Staumauern), oder wenn Feuchteprozesse in Bauteilen auftreten, die den Transport von schädlichen Stoffen ermöglichen. Mittels handelsüblicher multifunktionaler Sensoren wie einem Bluetooth Low Energy (BLE) wurden erste Machbarkeitsuntersuchungen zur Massenfeuchtemessung durchgeführt. Die untersuchten Parameter sind der sogenannte Received Signal Strength Indicator (RSSI) eines Bluetooth‐Low‐Energy(BLE)‐Signals und die abgestrahlte Sendeleistung eines eingebetteten BLE‐Moduls. Das BLE‐Modul kommuniziert mit einem mobilen smarten Empfänger wie einem Tablet oder einem Smartphone als SmartHub. Die Veränderung des RSS‐Indikators ist ein Maß für die Veränderung der Feuchte im Bauteil, während die ausgesandte Signalstärke konstant bleibt. Wenn das BLE‐Signal einen feuchten Bereich im Baumaterial durchläuft, verändert sich der RSS‐Indikator. Nach erfolgreicher Machbarkeitsstudie wurde nun ein aus dem Netzwerk MI4G (Modern Industry for Germany) zwischen der Bundesanstalt für Materialprüfung und dem Klein‐ und mittelständigen Unternehmen LinTech GmbH heraus beantragtes ZIM‐Projekt bewilligt. Der Bericht zeigt erste Ergebnisse von Voruntersuchungen und gibt einen Ausblick auf weitere Forschungsaktivitäten.
Festakt zur Verleihung des Deutschen Brückenbau preises 2016 und 26. Dresdner Brückenbausymposium
Authors: Stritzke Jürgen 
Published: October 2016

AbstractAm 14. März d. J. war es wieder so weit: In einem großartigen Festakt wurde der Deutsche Brückenbaupreis 2016 vor über 1 200 Gästen im Hörsaalzentrum der Technischen Universität Dresden verliehen. Diesen bedeutenden Ingenieurpreis hatte die Bundesingenieurkammer gemeinsam mit dem Verband Beratender Ingenieure (VBI) bereits zum sechsten Mal für herausragende Leistungen auf dem Gebiet des Brückenbaus ausgelobt.Er ging auch diesmal an ganzheitlich gelungene Brückenbauwerke in den beiden Kategorien „Straßen‐ und Eisenbahnbrücken” sowie „Fuß‐ und Radwegbrücken”, die Beiträge zur Baukultur leisten. Die Ingenieure, deren kreative Leistungen maßgeblich zum Entstehen der ausgezeichneten Brückenbauwerke geführt haben, erhielten die Preisskulptur [1]. Der alle zwei Jahre vergebene Ingenieurpreis der Königsdisziplin des Ingenieurbaus steht unter der Schirmherrschaft des BMVI. Hauptsponsor ist die Deutsche Bahn AG. Durch den Festakt führte die vom TV‐Sender rbb bekannte Moderatorin Petra Gute.
Leichte Dachschalen aus Carbonbeton
Authors: Scholzen Alexander , Chudoba Rostislav , Hegger Josef , Will Norbert 
Published: October 2016

AbstractFür einfach gekrümmte Dachschalen aus Textilbeton wurde eine Herstellmethodik entwickelt, die sowohl den Produktionsprozess im Fertigteilwerk als auch materialgerechte Lösungsansätze für Transport und Montage der filigranen Betonschalen auf der Baustelle umfasst. Darüber hinaus wurde anhand von Prototypen die statische Leistungsfähigkeit der Dachschalen im Bauteilversuch verifiziert und über Sägeschnitte die hohe Ausführungsqualität der hergestellten Fertigteile dokumentiert. Die Anwendung der entwickelten Methoden wurde bei einer Dachkonstruktion aus fünf Carbonbeton‐Dachschalen erfolgreich demonstriert. Die Bauteildicke der 10 m2 großen Fertigteile betrug lediglich 2 cm und unterstreicht so eindrucksvoll die Leistungsfähigkeit und das architektonische Anwendungspotenzial des neuartigen Kompositwerkstoffs.
Luminescence characterization of Dy and Eu doped Na6Mg(SO4)4 phosphors
Authors: Kore Bhushan P. , Pardhi Shilpa A. , Dhoble N. S. , Dhoble S. J. , Swart H. C. 
Published: October 2016

AbstractA series of single‐phase phosphors based on Na6Mg(SO4)4 (Zeff = 11.70) doped with Dy and Eu was prepared by the wet chemical method. The photoluminescence (PL) and thermoluminescence (TL) properties of Dy3+‐ and Eu3+‐activated Na6Mg(SO4)4 phosphors were investigated. The characteristic emissions of Dy3+ and Eu3+ were observed in the Na6Mg(SO4)4 host. The TL glow curve of the Na6Mg(SO4)4:Dy phosphor consisted of a prominent peak at 234°C and a very small hump at 158°C. The TL sensitivity of the Na6Mg(SO4)4:Dy phosphor was found to be four times less than the commercialized CaSO4:Dy phosphor. The TL dose–response of the Na6Mg(SO4)4:Dy phosphor was studied from a dose range of 5–10 kGy and the linear dose–response was observed up to 1 kGy which is good for a microcrystalline phosphor. Trapping parameters for both the samples were calculated using the Initial Rise and Chen's peak shape methods.
In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters
Authors: Jiao Song , Li Xu , Yu Huimin , Yang Huan , Li Xue , Shen Zhongyao 
Published: October 2016

ABSTRACTSurfactin‐family lipopeptides are green biosurfactants with substantial industrial potential. The major problem prohibiting surfactin use is the low titer of the wild producer, Bacillus subtilis. Using transcriptomic analysis, four strong promoters, PgroE, Pcdd, PrplK, and PsspE, were identified and cloned from the genome of B. subtilis THY‐7, a novel surfactin producer that has been identified from soil with a 0.55 g/L surfactin titer. An optimal promoter, PgroE, was selected to replace the native THY‐7 surfactin synthase (SrfA) promoter through single‐cross homologous recombination; however, the resulting engineered strain containing the PgroE substitution did not synthesize surfactin. The sucrose‐inducible promoters PsacB and PsacP were then substituted in place of PsrfA, and the resulting engineered strains produced 1.09 and 0.22 g/L surfactin, respectively. An artificial, sucrose‐inducible Pg1 promoter was produced through fusion of the PgroE and PsacB ribonucleic antiterminator (RAT), and the engineered strain containing the Pg1‐substitution produced a surfactin titer of 1.44 g/L. An artificial IPTG‐inducible promoter, Pg2, was constructed from a PgroE‐lacO fusion and then substituted for the chromosomal PsrfA locus, and the surfactin titer of the resulting THY‐7/Pg2‐srfA increased to 5.98 g/L. The driving capacity of Pg2 was further improved by the inclusion of two point mutations in the −35 and −10 regions to produce the novel promoter Pg3. Pg3 exhibited super‐strong activity as measured by lacZ reporter gene overexpression (approximately 3000 U). The Pg3‐substitution strain THY‐7/Pg3‐srfA produced up to 9.74 g/L surfactin in a 5 L fermentor. The maximum productivity was 0.30 g/L/h, and the greatest yield reached 0.14 g surfactin/g sucrose. Biotechnol. Bioeng. 2017;114: 832–842. © 2016 Wiley Periodicals, Inc.
Meta-analysis of the use of surgical sealants for suture-hole bleeding in arterial anastomoses
Authors: Rogers A C , Turley L P , Cross K S , McMonagle M P 
Published: October 2016

AbstractBackgroundSuture-hole bleeding in arterial anastomoses prolongs operating time and increases blood loss, particularly with the use of prosthetic grafts. Surgical sealants (such as fibrin) may be used as haemostatic adjuncts in vascular surgery. This is a systematic review and meta-analysis of published studies that investigated the utility of surgical sealants in arterial-to-prosthetic graft anastomoses.MethodsA systematic review was undertaken of papers published until January 2015 on Embase, MEDLINE, PubMed, PubMed Central and Cochrane databases that analysed the use of surgical sealants as haemostatic adjuncts after arterial anastomoses. RCTs were included, with study endpoints of time to haemostasis or haemostasis at 5 min. Secondary outcomes included treatment failure, mean difference in estimated blood loss and duration of surgery. Sensitivity and subgroup analyses were performed, as well as funnel plot analysis for publication bias.ResultsA total of 2513 citations were reviewed; 19 RCTs comprising 1560 patients were ultimately included in the analysis. The majority of studies compared fibrin sealant with control haemostatic measures. Pooled analysis suggested that surgical sealants reduced the time to haemostasis (mean difference 243·26 (95 per cent c.i. 183·99 to 302·53) s; P &lt; 0·001), improved haemostasis at 5 min (odds ratio 4·50, 95 per cent c.i. 2·59 to 7·81; P &lt; 0·001), and were associated with less treatment failure, blood loss and shorter duration of surgery.ConclusionSurgical sealants appear to reduce suture-hole bleeding significantly in vascular prosthetic graft anastomoses compared with standard haemostatic measures.
Recent analytical methods for atmospheric polycyclic aromatic hydrocarbons and their derivatives
Authors: Hayakawa Kazuichi , Tang Ning , Toriba Akira 
Published: October 2016

AbstractPolycyclic aromatic hydrocarbons (PAHs) and nitropolycyclic aromatic hydrocarbons (NPAHs) are ubiquitous environmental pollutants. Moreover, some oxidative metabolites of these pollutants, such as hydroxylated and epoxide PAHs, cause endocrine disruption or produce reactive oxygen species. These compounds have become a large concern from the viewpoint of particulate matter (PM2.5) pollution. This report deals with recent studies concerning analytical methods for PAHs, NPAHs and related compounds in atmospheric and biological samples.
Homoharringtonine combined with aclarubicin and cytarabine synergistically induces apoptosis in t(8;21) leukemia cells and triggers caspase‐3‐mediated cleavage of the AML1‐ETO oncoprotein
Authors: Cao Jiang , Feng Hao , Ding Ning‐Ning , Wu Qing‐yun , Chen Chong , Niu Ming‐Shan , Chen Wei , Qiu Ting‐Ting , Zhu Hong‐Hu , Xu Kai‐Lin 
Published: October 2016

AbstractHomoharringtonine combined with aclarubicin and cytarabine (HAA) is a highly effective treatment for acute myeloid leukemia (AML), especially for t(8;21) AML. However, the underlying mechanisms by which HAA kills t(8;21) AML cells remain unclear. In this study, SKNO‐1 and Kasumi‐1 cells with t(8;21) were used. Compared with individual or pairwise administration of homoharringtonine, aclarubicin, or cytarabine, HAA showed the strongest inhibition of growth and induction of apoptosis in SKNO‐1 and Kasumi‐1 cells. HAA caused cleavage of the AML1‐ETO (AE) oncoprotein to form truncated AE (ΔAE). Pretreatment with the caspase‐3 inhibitor caspase‐3 inhibitor Q‐DEVD‐OPh (QDO) not only suppressed HAA‐induced apoptosis but also abrogated the cleavage of AE and generation of ΔAE. These results suggest that HAA synergistically induces apoptosis in t(8;21) leukemia cells and triggers caspase‐3‐mediated cleavage of the AML1‐ETO oncoprotein, thus providing direct evidence for the strong activity of HAA toward t(8;21) AML.
Minding the gaps: Using narrative accounts to explore people's experiences of using North Staffs MIND's Adult Counselling Service
Authors: Beaumont Elaine , Hickey Anthony Joseph , McAndrew Sue , Goldman Stacey , Warne Tony 
Published: October 2016

AbstractIntroductionIn the UK, almost 50% of illness diagnosed among working age adults is mental distress, with depression and chronic anxiety cited as the two most prevalent psychological illnesses. However, only 24% of those who experience anxiety and depression, consistent with diagnoses, receive National Health Service (NHS) interventions. Effective mental health care is predicated on understanding the lived experiences of those using services in order to provide sensitively attuned therapy. An understanding of the process of counselling and what makes it effective will only be achieved through hearing the voices of service users. However, with regard to counselling, the literature foregrounding the perspectives of those using services remains sparse.MethodThis study reports on a qualitative research project, the aim of which was to explore the experiences of people who have used North Staffs MIND’s Adult Counselling Service in order to elicit the strengths of and/or opportunities for improving the service. Twelve participants, five males and seven females, were interviewed on a one‐to‐one basis and six themes were identified; Mindful of the Gap; Easing Like Sunday Morning; Magic Moments; Love is in the Air; Lighting up a Future and Following up the Changes: Spreading the Word.ResultsFindings suggest the participants in this study found the service beneficial, with therapeutic interventions being tailor‐made to meet the person's needs, and one which ensures a safe environment and compassionate care for those in distress.
Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue
Authors: Cuetos Anibal , Steffen‐Munsberg Fabian , Mangas Sanchez Juan , Frese Amina , Bornscheuer Uwe T. , Höhne Matthias , Grogan Gideon 
Published: October 2016

AbstractPyridoxal‐phosphate (PLP)‐dependent enzymes catalyse a remarkable diversity of chemical reactions in nature. A1RDF1 from Arthrobacter aurescens TC1 is a fold type I, PLP‐dependent enzyme in the class III transaminase (TA) subgroup. Despite sharing 28 % sequence identity with its closest structural homologues, including β‐alanine:pyruvate and γ‐aminobutyrate:α‐ketoglutarate TAs, A1RDF1 displayed no TA activity. Activity screening revealed that the enzyme possesses phospholyase (E.C. 4.2.3.2) activity towards O‐phosphoethanolamine (PEtN), an activity described previously for vertebrate enzymes such as human AGXT2L1, enzymes for which no structure has yet been reported. In order to shed light on the distinctive features of PLP‐dependent phospholyases, structures of A1RDF1 in complex with PLP (internal aldimine) and PLP⋅PEtN (external aldimine) were determined, revealing the basis of substrate binding and the structural factors that distinguish the enzyme from class III homologues that display TA activity.
Predictors of emotional and physical dating violence in a sample of serious juvenile offenders
Authors: Sweeten Gary , Larson Matthew , Piquero Alex R. 
Published: October 2016

AbstractAimWe estimate group‐based dating violence trajectories and identify the adolescent risk factors that explain membership in each trajectory group.MethodUsing longitudinal data from the Pathways to Desistance Study, which follows a sample of 1354 serious juvenile offenders from Philadelphia, Pennsylvania and Phoenix, Arizona between mid‐adolescence and early adulthood, we estimate group‐based trajectory models of both emotional dating violence and physical dating violence over a span of five years in young adulthood. We then estimate multinomial logistic regression models to identify theoretically motivated risk factors that predict membership in these groups.ResultsWe identified three developmental patterns of emotional dating violence: none (33%), low‐level (59%) and high‐level decreasing (8%). The best‐fitting model for physical dating violence also had three groups: none (73%), low‐level (24%) and high‐level (3%). Race/ethnicity, family and psychosocial variables were among the strongest predictors of both emotional and physical dating violence. In addition, delinquency history variables predicted emotional dating violence and relationship variables predicted physical dating violence.ConclusionsDating violence is quite prevalent in young adulthood among serious juvenile offenders. Numerous predictors distinguish between chronic dating violence perpetrators and other groups. These may suggest points of intervention for reducing future violence. Copyright © 2016 John Wiley & Sons, Ltd.
Comparison of acute expansion of bioresorbable vascular scaffolds versus metallic drug‐eluting stents in different degrees of calcification: An optical coherence tomography study
Authors: Ming Fam Jiang , van Der Sijde Johannes N , Karanasos Antonios , Felix Cordula , Diletti Roberto , van Mieghem Nicolas , de Jaegere Peter , Zijlstra Felix , Jan van Geuns Robert , Regar Evelyn 
Published: October 2016

ObjectivesThe acute expansion of bioresorbable vascular scaffolds (BRS) and drug‐eluting stents (DES) in lesions with different extent of calcification was compared by Optical Coherence Tomography (OCT).BackgroundThe acute mechanical performance of polymeric BRS in calcified lesions is poorly understood.MethodsAcute device performance in lesions treated with either BRS(N = 50) or DES (N = 50) was compared using Optical Coherence Tomography (OCT). According to angiographic degree of calcification the lesions were divided in three groups: no/mild, moderate and heavy calcification. Device performance was assessed with the following parameters by OCT: mean scaffold area, eccentricity index (EI), symmetry index (SI) and percentage incomplete strut apposition (ISA).ResultsOne hundred lesions from 85 patients (BRS/DES; 37/48) were analyzed. Scaffold area and SI were similar between BRS and DES groups in the three calcification subgroups. Compared to DES, EI in BRS was marginally lower in the no/mild calcification group (0.86 ± 0.03 versus 0.88 ± 0.03, p = 0.018) but was similar in the moderate and heavy calcification groups. Compared to DES, percentage ISA struts in BRS was similar in the no/mild calcification group and was significantly lower in the moderate and heavy calcification groups (2.96 ± 2.36 versus 6.78 ± 4.61%, p = 0.002 and 1.82 ± 2.40 versus 8.89 ± 8.25%, p = 0.025 respectively).ConclusionsWith adequate lesion preparation, implantation of BRS in a population reflective of clinical practice, resulted in a similar luminal gain compared to DES as measured by OCT, regardless of the degree of angiographic calcification, while acute malapposition is lower with BRS in moderately and heavily calcified lesions. The clinical significance of our findings warrants further evaluation in future studies. © 2016 Wiley Periodicals, Inc.
Functional salivary reservoir in maxillary complete denture – technique redefined
Authors: Joseph Angel Mary , Joseph Suja , Mathew Nicholas , Koshy Ashwin Thomas 
Published: October 2016

Key Clinical MessageComplete dentures are poorly tolerated in patients with xerostomia. A salivary reservoir can be incorporated into a denture that provides slow, sustained, and continuous release of salivary substitute. This article describes a simple, cost‐effective, and innovative technique of fabrication and designing of functional maxillary salivary reservoir complete denture.