bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Genetic Screen for Pre-mRNA Splicing Mutants of Arabidopsis thaliana Identifies Putative U1 snRNP Components RBM25 and PRP39a
Authors: Kanno Tatsuo , Lin Wen-Dar , Fu Jason L , Chang Chia-Liang , Matzke Antonius J M , Matzke Marjori 
Published: October 2017

Abstract
In a genetic screen for mutants showing modified splicing of an alternatively spliced GFP reporter gene in Arabidopsis thaliana, we identified mutations in genes encoding the putative U1 small nuclear ribonucleoprotein (snRNP) factors RBM25 and PRP39a. The latter has not yet been studied for its role in pre-messenger RNA (pre-mRNA) splicing in plants. Both proteins contain predicted RNA-binding domains and have been implicated in 5′ splice site selection in yeast and metazoan cells. In rbm25 mutants, splicing efficiency of GFP pre-mRNA was reduced and GFP protein levels lowered relative to wild-type plants. By contrast, prp39a mutants exhibited preferential splicing of a U2-type AT-AC intron in GFP pre-mRNA and elevated levels of GFP protein. These opposing findings indicate that impaired function of either RBM25 or PRP39a can differentially affect the same pre-mRNA substrate. Given a prior genome-wide analysis of alternative splicing in rbm25 mutants, we focused on examining the alternative splicing landscape in prp39a mutants. RNA-seq experiments performed using two independent prp39a alleles revealed hundreds of common genes undergoing changes in alternative splicing, including PRP39a itself, a second putative U1 snRNP component PRP40b, and genes encoding a number of general transcription-related proteins. The prp39a mutants displayed somewhat delayed flowering, shorter stature, and reduced seed set but no other obvious common defects under normal conditions. Mutations in PRP39b, the paralog of PRP39a, did not visibly alter GFP expression, indicating the paralogs are not functionally equivalent in this system. Our study provides new information on the contribution of PRP39a to alternative splicing and expands knowledge of plant splicing factors.
Mutation Rate Evolution in Partially Selfing and Partially Asexual Organisms
Authors: Gervais Camille , Roze Denis 
Published: October 2017

Abstract
Different factors can influence the evolution of the mutation rate of a species: costs associated with DNA replication fidelity, indirect selection caused by the mutations produced (that should generally favor lower mutation rates, given that most mutations affecting fitness are deleterious), and genetic drift, which may render selection acting on weak mutators inefficient. In this paper, we use a two-locus model to compute the strength of indirect selection acting on a modifier locus that affects the mutation rate toward a deleterious allele at a second, linked, locus, in a population undergoing partial selfing or partial clonality. The results show that uniparental reproduction increases the effect of indirect selection for lower mutation rates. Extrapolating to the case of a whole genome with many deleterious alleles, and introducing a direct cost to DNA replication fidelity, the results can be used to compute the evolutionarily stable mutation rate, U. In the absence of mutational bias toward higher U, the analytical prediction fits well with individual-based, multilocus simulation results. When such a bias is added into the simulations, however, genetic drift may lead to the maintenance of higher mutation rates, and this effect may be amplified in highly selfing or highly clonal populations due to their reduced effective population size.
Association between risky behaviors in adolescents and altered psychophysiological emotional responses
Authors: Galindo-Aldana Gilberto Manuel , Fraga-Vallejo Miguel , Menchaca-Díaz Rufino , Alvelais-Alarcón Marina , Machinskaya Regina 
Published: October 2017

Introducción. La psicofisiología de la respuesta emocional en la adolescencia es un factor clave en la comprensión de conductas de riesgo y desadaptación.Objetivo. Comparar especificidades de la reacción psicofisiológica emocional entre adolescentes con y sin conductas de riesgo.Materiales y métodos. Las medidas consistieron en la caracterización de conductas de riesgo (CR), variaciones en la frecuencia cardiaca (FC) electromiografía (EMG), conductancia de la piel (CP) y temperatura durante la presentación de estímulos emocionales visuales, así como la calificación en parámetros de valencia emocional, activación y dominancia de dichos estímulos.Resultados. Se encontraron diferencias significativas entre los adolescentes con CR comparados con aquellos que no las presentaban: se detectaron signos de mayor reacción psicofisiológica a los estímulos emocionales.Conclusiones. El grupo control se caracterizó por una bradicardia inicial más expresiva en comparación con el grupo con CR, ambos grupos mostraron desaceleración cardíaca más pronunciada ante estímulos desagradables, niveles más altos en EMG ante estímulos agradables en el grupo con RB e indicadores más elevados de CP ante los tres tipos de estímulo. Se requiere un estudio de cohorte a fin de comparar los hallazgos actuales en CR frente a la reacción fisiológica emocional entre los grupos de adolescentes.
Intervención integral de ocho meses disminuye el peso y mejora los niveles de depresión y ansiedad en obesos severos y mórbidos
Authors: Cofre-Lizama Alfonso , Delgado-Floody Pedro , Angulo-Díaz Pamela , Jérez-Mayorga Daniel 
Published: October 2017

Introducción. La obesidad mórbida genera una disminución considerable de la expectativa y calidad de vida, según lo cual existen niveles aumentados de depresión y ansiedad.Objetivo. Determinar los efectos de un programa de tratamiento integral en la pérdida de peso y los niveles de ansiedad y depresión de obesos severos y mórbidos.Materiales y métodos. Se estudiaron 4 hombres y 12 mujeres de un promedio de 34 26 años, candidatos a cirugía bariátrica. 6 obesos severos y 10 pacientes con obesidad mórbida participaron en una intervención de tratamiento integral de 8 meses. Se evaluaron en preintervención y post intervención: peso, índice de masa corporal (IMC), contorno de la cintura, capacidad cardiorrespiratoria, ansiedad y depresión.Resultados. El peso y el IMC posterior a la intervención disminuyeron de forma significativa (p<0.001) en un 12.28% y 12.30%, respectivamente, el contorno cintura presentó una disminución de un 11.67% (p< 0.001). Además, la capacidad respiratoria mejoró bastante (p<0.001). Los síntomas ansiosos y depresivos disminuyeron de manera significativa (p<0.001).Conclusión. La intervención integral de 8 meses presenta beneficios significativos en la pérdida de peso de los participantes y en la mejora de los niveles de ansiedad y depresión. Por tal motivo, la intervención realizada puede recomendarse para el tratamiento de esta condición.
Evaluation of complications in postoperative of thirdmolar extraction
Authors: Costa Morgana Marielle de Almeida , De Sousa Layane Maria , De Moura Leonardo Alonso 
Published: October 2017

OBJECTIVE: This study aimed to analyze parameters such as the presence of trismus, facial swelling, postoperative pain, paresthesia of the inferior alveolar and lingual nerve, alveolitis, late bleeding and correct use of prescribed medications. Relating the complication rates with age, gender and proper use of medications prescribed.METHODS: This study evaluated 20 patients who have gone through removal of third molars, using questionnaires applied by researchers in the postoperative period of seven days in the graduate and professional qualifications Pós-Doc center from April to May/2015.RESULTS: It was observed that 45% of the patients had pain, 45% had trismus, 15% had paresthesia, 85% had late light bleeding, 5% had fever, malaise or discharge from the surgical site and 45% had facial swelling.CONCLUSION: The more frequent complications found were pain and late bleeding, with no association between pain and gender and pain and age.
Radiographic assessment of the quality of dental restorations and their relationship with periodontal radiographic changes
Authors: Fortes João Henrique Parise , Dallavilla Felipe Guarda , Tirapelli Camila , Watanabe Plauto Christopher Aranha , Brito Ana Caroline Ramos , De Oliveira-Santos Christiano 
Published: October 2017

INTRODUCION: The quality of dental restorations can be directly related to the maintenance of periodontal health. Particularly in relation to the contour of interproximal restorations, radiographs allow the assessment of possible excess or lack of proximal contact of restorative materials.OBJECTIVE: To evaluate the frequency of adequate and inadequate restorations, the type of restorative material and the relationship of these parameters with periodontal alterations.METHODS: One hundred digital panoramic radiographs were evaluated, with interproximal restorations being observed on these examinations, classifying them according to the material used and the quality of interproximal restoration and the presence of periodontal alterations. Thepossible correlation between these parameters was evaluated.RESULTS: The frequency of adequate restorations was 48% of restored proximal surfaces. Among the inadequate restorations, 46.2% and 53.8% were over-contour and lack of proximal contact, respectively. The restorations were metallic in 64% of cases.CONCLUSIONS: Over-contour were more common among metal restorations and lack of proximal contact was more frequent among non-metal restorations. The presence of restorations was associated with increased prevalence of periodontal alterations.
Dental emergencies in public health services: what is the role of the Dental Surgeon?
Authors: Rios Leonardo Essado , Queiroz Maria Goretti 
Published: October 2017

OBJECTIVE: To assess the opinions of Brazilian dental surgeons (DS) about their roles in Public Emergency Dental Services (PEDS).METHODS: A cross-sectional study was conducted with an intentional sample of 44 DS who worked at the PEDS in a large municipality in the central-western region of Brazil, in 2013. To collect data we designed, pretested and applied a self-administered questionnaire with Likert scale assertions about the DS duties, referral of patients and execution of dental procedures in PEDS. Post-hoc tests revealed an instrument’s reliability of 91%.RESULTS: As their main duty, DS pointed out providing relief to patients’ complaints in cases of dental pain and trauma. Regarding referral of patients, they considered typically that they should refer to services at a higher complexity level those patients exhibiting infections in advanced stages under risk of disseminations. Most of them disagreed with performing dental restorations in emergency consultations, while the majority stated that they should mainly perform intraoral abscesses drainage, alveolitis and haemorrhage treatment.CONCLUSION: To provide pain and trauma relief to patients was pointed out by DS as their key role in PEDS, as well as to refer at-risk patients to high-level complexity services and to perform procedures aimed at solving emergency situations, instead of providing restorative treatment to patients.
Analysis of the frequency of radiation therapy complications in patients with prostate cancer
Authors: Isaev I G , Guliev F A , Akperov K S , Alieva N R 
Published: October 2017

Aim. Study of the frequency of radiation therapy complications in patients with prostate cancer.
Methods. The study included 142 patients who underwent radiation therapy combined with hormonal therapy in 2001-2015. Average age of patients was 68 years. All of them received 3D-conformal radiotherapy by linear accelerators with 6 to 15 MV photon beams. Single boost dose was 2 Gy, total boost dose was 75 Gy. We studied frequency and severity of acute hematological toxicity and early and late gastroenterological and urological complications of the treatment. Treatment-related toxicity was assessed by RTOG/ EORTC and CTCAE v. 4.0 scales.
Results. Early radiation-induced proctitis of I, II and III degree were registered in 95 (66.9%), 32 (22.5%) and 8 (5.6%) patients, respectively. Acute cystitis of I, II and III degree were noted in 22 (15.5%), 87 (61.3%) and 16 (11.3%) patients, respectively. Signs of late radiation-induced proctitis of I degree was diagnosed in 21 (14.8%) cases. Late radiation-induced cystitis of I degree was diagnoswed in 37 (26.1%) patients. No late complications of higher severity occurred.
Conclusion. The results of the conducted study showed that use of high-tech precision external-beam radiotherapy techniques in the treatment of prostate cancer along with high efficacy allows to reduce the number of early and late complications.
Health features of newborns depending on vitamin D level of their mothers during pregnancy
Authors: Vasil’eva E N , Mal’tseva L I , Denisova T G , Gerasimova L I 
Published: October 2017

Aim. Evaluation of health state in newborns whose mothers were at risk of pre-eclampsia, depending on vitamin D level.
Methods. Study materials included peripheral blood from pregnant women and umbilical cord blood of newborns. Vitamin D level was measured by enzyme-linked immunoassay kits of BIOMEDICAGRUPPE company (Germany). Intrauterine fetal state was studied with the use of ultrasonography.
Results. Analysis of the study proved that vitamin D level in the umbilical cord blood correlates with its concentration in the mother’s blood. In women at pre-eclampsia risk decreased calcium level and vitamin D deficiency in the blood were detected. Administration of 2,000 IU of vitamin D and 1.5 g of calcium from the beginning of 2nd trimester of pregnancy resulted in reduction of frequency and severity of pre-eclampsia and its complications, including intrauterine growth retardation. Use of vitamin D in combination with calcium supplementation was shown to significantly improve unfavorable perinatal outcomes in women at high risk of pre-eclampsia, reducing by 3 times hypoxia and cerebral fetal lesions frequency that proves the vitamin D importance for functioning of mother-placenta-fetus system.
Conclusion. Children of patients at risk of pre-eclampsia not taking vitamin D and calcium supplementation have lower birth weight and Apgar score, they have more frequent perinatal complications.
Electromyographic study in spondylogenic monocorditis
Authors: Alimetov Kh A , Rodnyakova E B 
Published: October 2017

Aim. To reveal relation between neuromascular disorders of the larynx and cervical spine pathology and to assess extrinsic laryngeal muscular tension in digital format.
Methods. The article contains clinical experience of the survey of 130 patients, 110 of whom were included into the study group with diagnosed spondylogenic monocorditis, and 20 healthy subjects were included into control group. All patients underwent electromyographic study using the patented Kh.A. Alimetov аnd D.D. Jabarov’s technique with the use of 4-channel electromyograph Neuro-MEP (Neurosoft) with the frequency range from 5 Hz to 20 kHz. The used method of interference (global) electromyography allowed obtaining information on laryngeal muscular tension in norm and spondylogenic pathology.
Results. In patients with spondylogenic monocorditis, the upper and lower muscle group tension was increased approximately by 1.2 times on the affected side in contrast to the opposite side. The received results confirm clinical trial data - on the affected side the vocal fold is visually thinner than the opposite fold, lags behind it during phonation and has decreased tension, and the increased tension of anterior laryngeal muscles on the right or left side, revealed during examination and palpation of the larynx, indicates neuromuscular disorder in cervical osteochondrosis on the affected side and involvement of certain laryngeal regions in the process of myofixation.
Conclusion. Electromyographic study provides quantitative assessment of laryngeal muscular tension imbalance, allows to correctly prescribe treatment and to control it.
Some aspects of realizing the order for ophtalmologic service as exemplified by Irkutsk oblast
Authors: Zapevalin P V , Taevsky B V 
Published: October 2017

Aim. Evaluation of the state of ophthalmologic service in Irkutsk oblast, as well as its compliance with the requirements of new legislative and regulatory documents of the federal level, development of a draft program for the development of ophthalmic care for the adult population of Irkutsk oblast.
Methods. To achieve the goal, two types of special process chart («Personnel» and «Equipment») were developed, based on the technology of a fact-potential method for assessing the performance of health facilities. Information capabilities of these methods were demonstrated. Assessment of comliance of the human resources and material and technical equipment of the ophthalmologic service of Irkutsk oblast with the order for medical care was performed.
Results. A serious staff shortage was reveled, as well as unsatisfactory material and technical equipment of institutions providing ophthalmologic service to the adult population. This problem is especially acute in the regions of Irkutsk oblast. To bring the material and technical base of the ophthalmologic service of Irkutsk oblast into compliance with the requirements of the Law №323 FZ and the approved Order for medical care to the adult population in case of eye diseases, 169.3 million rubles are required, of which 145 million rubles are required for «priority expenses». Measures aimed at improving the state of the ophthalmologic service in the region were suggested. The need to develop and implement a perspective regional targeted program for the development of ophthalmologic service of the Irkutsk oblast for a long-term period was shown.
Conclusion. Human resources and material and technical equipment of medical institutions providing ophthalmologic service to the population of Irkutsk oblast do not comply with the standards; one of the solutions to the problem is the development and implementation of the regional targeted program for the development of ophthalmologic service.
MOBILIDADE NA MARCHA, RISCO DE QUEDAS E DEPRESSÃO EM IDOSOS INSTITUCIONALIZADOS E NÃO INSTITUCIONALIZADOS
Authors: Costa Camila , Kemer Carla Gabriela , Oliveira Daniel Vicentini , Antunes Mateus Dias , Nascimento Júnior José Roberto Andrade do , Silva Cristina Cristovão Ribeiro da 
Published: October 2017

Este artigo tem como objetivo comparar o risco de quedas, indicativos de depressão e mobilidade na marcha de idosos institucionalizados e idosos frequentadores de um centro de convivência do município de Foz do Iguaçu, estado do Paraná. Trata-se de um estudo transversal, constituído por 26 idosos de ambos os sexos, sendo 13 institucionalizados (ILPI) e 13 frequentadores de um centro de convivência (CCI). Foi utilizado um questionário sociodemográfico, a Escala de Equilíbrio de Berg, escala de depressão geriátrica e o teste Timed Up and go. A análise dos dados foi realizada mediante os testes Shapiro-Wilk, “U” de Mann-Whitney, a correlação de Spearman e o teste Qui-quadrado e o teste exato de Fisher. Foi adotada a significância de p < 0,05. Ressalta-se que, significativamente, idosos do CCI apresentaram menor indicativo de depressão (p = 0,009), menor risco de quedas (p = 0,001) e melhor mobilidade na marcha (p = 0,001) em detrimento dos idosos do ILPI. Após a identificação de que os idosos institucionalizados apresentam maior risco de quedas, dificuldade na marcha e de sinais depressivos quando comparados aos não institucionalizados, surge um desafio para os serviços de saúde no enfrentamento da incapacidade funcional ao longo da vida.
Editorial
Authors: Santos Gildenir Carolino 
Published: October 2017

Estamos aqui, novamente, com mais um número da ETD — Educação Temática Digital! É com muita satisfação que os colocamos sintonizados com o que está acontecendo no momento com as pesquisas e os trabalhos no campo educacional, envolvendo não somente uma área temática, mas as diversas temáticas que agregam a educação. Nesta edição, apresentaremos 24 trabalhos que têm como tema central “Diferenças e Subjetividade”, assim distribuídos: 8 trabalhos na Seção Artigos; 12 na seção Dossiê; 2 na Seção Pesquisa; e 2 na Seção Relatos de experiência.
UNIVERSIDADE E CRISE INSTITUCIONAL: perspectivas de uma formação humana
Authors: Pinho Maria José de 
Published: October 2017

A universidade brasileira do século XXI tem enfrentado adversidades multidimensionais no âmbito do ensino e da pesquisa. Sua estabilidade tem percorrido um labirinto de inferências que a impede de adentrar o espaço educativo para se desenvolver em um processo educacional que atenta à sua função social e humanística, perante uma sociedade desacreditada de possibilidades superadoras. Nesse sentido, buscamos fazer uma reflexão da transição do paradigma conservador para paradigma emergente e sua influência no contexto da universidade do século XXI na perspectiva de uma visão complexa da realidade contemporânea. Buscamos contextualizar a universidade como protagonista na construção de um novo paradigma para educação. O repensar das instituições de ensino superior a partir do Processo de Bolonha tendo em vista uma educação superior voltada para competitividade e para a competência profissional. Os desafios da universidade para este século tanto nos aspectos filosóficos e epistemológicos na percepção da complexidade humana e da pertinência do conhecimento mediante a superação do conhecimento fragmentado e compartimentalizado, colaborando para o autoconhecimento e para o diálogo entre os saberes.
PADRÕES ESTÉTICOS E ATUAÇÃO PROFISSIONAL DE MULHERES TELEJORNALISTAS: uma pesquisa exploratória
Authors: Martino Luis Mauro Sá , Zancoper Julya Vendite 
Published: October 2017

Embora a beleza não esteja entre os pré-requisitos da prática jornalística, , ela parece ser um elemento importante na escolha de apresentadora de telejornais. Este artigo, ponto inicial de uma pesquisa em desenvolvimento, delineia alguns aspectos dessa demanda, colocada para as jornalistas, de atingir um determinado padrão hegemônico de beleza no telejornalismo. A partir do observação das apresentadoras dos principais telejornais da TV aberta, nota-se a presença de um padrão – apresentadoras brancas, de cabelo castanho, dentro de um tipo físico considerado “magro”, com pouco espaço para jornalistas fora desse modelo. Esse padrão é discutido a partir do ponto de vista de questões de identidade e poder simbólico.