bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toward a Complete North American Borrelia miyamotoi Genome
Authors: Kingry Luke C. , Replogle Adam , Batra Dhwani , Rowe Lori A. , Sexton Christopher , Dolan Marc , Connally Neeta , Petersen Jeannine M. , Schriefer Martin E. 
Published: February 2017

ABSTRACT

Borrelia miyamotoi
, of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere. Here, we present the chromosome, eight extrachromosomal linear plasmids, and a draft sequence for five circular and one linear plasmid of a
Borrelia miyamotoi
strain isolated from an
Ixodes
sp. tick from Connecticut, USA.
Draft Genome Sequence of Acholeplasma laidlawii, a Common Contaminant of Cell Cultures
Authors: Siqueira Franciele Maboni , Cibulski Samuel Paulo , Teixeira Thais Fumaco , Mayer Fabiana Quoos , Roehe Paulo Michel 
Published: February 2017

ABSTRACT

Mollicutes
are important cell culture contaminants which may eventually affect the results of biological assays or affect their interpretation.
Acholeplasma laidlawii
is one of the most frequent contaminants of cell cultures. Here, we report the complete genome sequence of
A. laidlawii
strain MDBK/IPV, recovered from Madin-Darby bovine kidney (MDBK) cells.
Impaired peripheral vasodilation during graded systemic hypoxia in healthy older adults: role of the sympathoadrenal system
Authors: Richards Jennifer C. , Crecelius Anne R. , Larson Dennis G. , Luckasen Gary J. , Dinenno Frank A. 
Published: February 2017

Systemic hypoxia is a physiological and pathophysiological stress that activates the sympathoadrenal system and, in young adults, leads to peripheral vasodilation. We tested the hypothesis that peripheral vasodilation to graded systemic hypoxia is impaired in older healthy adults and that this age-associated impairment is due to attenuated β-adrenergic mediated vasodilation and elevated α-adrenergic vasoconstriction. Forearm blood flow was measured (Doppler ultrasound), and vascular conductance (FVC) was calculated in 12 young (24 ± 1 yr) and 10 older (63 ± 2 yr) adults to determine the local dilatory responses to graded hypoxia (90, 85, and 80% O2 saturations) in control conditions, following local intra-arterial blockade of β-receptors (propranolol), and combined blockade of α- and β-receptors (phentolamine + propranolol). Under control conditions, older adults exhibited impaired vasodilation to hypoxia compared with young participants at all levels of hypoxia (peak ΔFVC at 80% [Formula: see text] = 4 ± 6 vs. 35 ± 8%; P < 0.01). During β-blockade, older adults actively constricted at 85 and 80% [Formula: see text] (peak ΔFVC at 80% [Formula: see text] = −13 ± 6%; P < 0.05 vs. control), whereas the response in the young was not significantly impacted (peak ΔFVC = 28 ± 8%). Combined α- and β-blockade increased the dilatory response to hypoxia in young adults; however, older adults failed to significantly vasodilate (peak ΔFVC at 80% [Formula: see text]= 12 ± 11% vs. 58 ± 11%; P < 0.05). Our findings indicate that peripheral vasodilation to graded systemic hypoxia is significantly impaired in older adults, which cannot be fully explained by altered sympathoadrenal control of vascular tone. Thus, the impairment in hypoxic vasodilation is likely due to attenuated local vasodilatory and/or augmented vasoconstrictor signaling with age. NEW & NOTEWORTHY We found that the lack of peripheral vasodilation during graded systemic hypoxia with aging is not mediated by the sympathoadrenal system, strongly implicating local vascular control mechanisms in this impairment. Understanding these mechanisms may lead to therapeutic advances for improving tissue blood flow and oxygen delivery in aging and disease.
The Landscape of Somatic Genetic Alterations in Metaplastic Breast Carcinomas
Authors: Ng Charlotte K.Y. , Piscuoglio Salvatore , Geyer Felipe C. , Burke Kathleen A. , Pareja Fresia , Eberle Carey A. , Lim Raymond S. , Natrajan Rachael , Riaz Nadeem , Mariani Odette , Norton Larry , Vincent-Salomon Anne , Wen Y. Hannah , Weigelt Britta , Reis-Filho Jorge S. 
Published: February 2017

Abstract
Purpose: Metaplastic breast carcinoma (MBC) is a rare and aggressive histologic type of breast cancer, predominantly of triple-negative phenotype, and characterized by the presence of malignant cells showing squamous and/or mesenchymal differentiation. We sought to define the repertoire of somatic genetic alterations and the mutational signatures of MBCs.
Experimental Design: Whole-exome sequencing was performed in 35 MBCs, with 16, 10, and 9 classified as harboring chondroid, spindle, and squamous metaplasia as the predominant metaplastic component. The genomic landscape of MBCs was compared with that of triple-negative invasive ductal carcinomas of no special type (IDC-NST) from The Cancer Genome Atlas. Wnt and PI3K/AKT/mTOR pathway activity was assessed using a qPCR assay.
Results: MBCs harbored complex genomes with frequent TP53 (69%) mutations. In contrast to triple-negative IDC-NSTs, MBCs more frequently harbored mutations in PIK3CA (29%), PIK3R1 (11%), ARID1A (11%), FAT1 (11%), and PTEN (11%). PIK3CA mutations were not found in MBCs with chondroid metaplasia. Compared with triple-negative IDC-NSTs, MBCs significantly more frequently harbored mutations in PI3K/AKT/mTOR pathway–related (57% vs. 22%) and canonical Wnt pathway–related (51% vs. 28%) genes. MBCs with somatic mutations in PI3K/AKT/mTOR or Wnt pathway–related genes displayed increased activity of the respective pathway.
Conclusions: MBCs are genetically complex and heterogeneous, and are driven by a repertoire of somatic mutations distinct from that of triple-negative IDC-NSTs. Our study highlights the genetic basis and the importance of PI3K/AKT/mTOR and Wnt pathway dysregulation in MBCs and provides a rationale for the metaplastic phenotype and the reported responses to PI3K/AKT/mTOR inhibitors in these tumors. Clin Cancer Res; 23(14); 3859–70. ©2017 AACR.
Aspirin-Induced Chemoprevention and Response Kinetics Are Enhanced by PIK3CA Mutations in Colorectal Cancer Cells
Authors: Zumwalt Timothy J. , Wodarz Dominik , Komarova Natalia L. , Toden Shusuke , Turner Jacob , Cardenas Jacob , Burn John , Chan Andrew T. , Boland C. Richard , Goel Ajay 
Published: February 2017

Abstract
This study was designed to determine how aspirin influences the growth kinetics and characteristics of cultured colorectal cancer cells that harbor a variety of different mutational backgrounds, including PIK3CA- and KRAS-activating mutations, and the presence or absence of microsatellite instability. Colorectal cancer cell lines (HCT116, HCT116 + Chr3/5, RKO, SW480, HCT15, CACO2, HT29, and SW48) were treated with pharmacologically relevant doses of aspirin (0.5–10 mmol/L) and evaluated for proliferation and cell-cycle distribution. These parameters were fitted to a mathematical model to quantify the effects and understand the mechanism(s) by which aspirin modifies growth in colorectal cancer cells. We also evaluated the effects of aspirin on key G0–G1 cell-cycle genes that are regulated by the PI3K–Akt pathway. Aspirin decelerated growth rates and disrupted cell-cycle dynamics more profoundly in faster growing colorectal cancer cell lines, which tended to be PIK3CA mutants. Additionally, microarray analysis of 151 colorectal cancer cell lines identified important cell-cycle regulatory genes that are downstream targets of PIK3 and were also dysregulated by aspirin treatment (PCNA and RB1). Our study demonstrated what clinical trials have only speculated, that PIK3CA-mutant colorectal cancers are more sensitive to aspirin. Aspirin inhibited cell growth in all colorectal cancer cell lines regardless of mutational background, but the effects were exacerbated in cells with PIK3CA mutations. Mathematical modeling combined with bench science revealed that cells with PIK3CA-mutations experience significant G0–G1 arrest and explains why patients with PIK3CA mutant colorectal cancers may benefit from aspirin use after diagnosis. Cancer Prev Res; 10(3); 208–18. ©2017 AACR.
Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury
Authors: Zhou Bisheng , Zeng Sheng , Li Nan , Yu Liming , Yang Guang , Yang Yuyu , Zhang Xinjian , Fang Mingming , Xia Jun , Xu Yong 
Published: February 2017

Objective—
Phenotypic modulation of vascular smooth muscle cells represents a hallmark event in vascular injury. The underlying mechanism is not completely sorted out. We investigated the involvement of angiogenic factor with G patch and FHA domains 1 (Aggf1) in vascular injury focusing on the transcriptional regulation of vascular smooth muscle cell signature genes.

Approach and Results—
We report here that Aggf1 expression was downregulated in several different cell models of phenotypic modulation in vitro and in the vessels after carotid artery ligation in mice. Adenovirus-mediated Aggf1 overexpression dampened vascular injury and normalized vascular smooth muscle cell signature gene expression. Mechanistically, Aggf1 interacted with myocardin and was imperative for the formation of a serum response factor–myocardin complex on gene promoters. In response to injurious stimuli, kruppel-like factor 4 was recruited to the Aggf1 promoter and enlisted histone deacetylase 11 to repress Aggf1 transcription. In accordance, depletion of kruppel-like factor 4 or histone deacetylase 11 restored Aggf1 expression and abrogated vascular smooth muscle cell phenotypic modulation. Finally, treatment of a histone deacetylase 11 inhibitor attenuated vascular injury in mice.

Conclusions—
Therefore, we have unveiled a previously unrecognized role for Aggf1 in regulating vascular injury.
Importing Notions in Health Law: Science and Proven Experience
Authors: Wahlberg Lena , Persson Johannes 
Published: February 2017

Abstract
In Swedish law, the notion of ‘science and proven experience’ (in Swedish, vetenskap och beprövad erfarenhet) defines the gold standard for public decision-making and practice, especially in medicine. The notion is notoriously vague but nevertheless plays an important role in the distribution of rights and duties of patients and healthcare workers. For example, failure to provide care in accordance with this standard can lead to penal responsibility. The notion also helps to define Swedish patients’ right to reimbursement for cross-border healthcare. From a legal point of view, the notion is especially intriguing because it appears to import medical standards into the legal conceptual apparatus. The purpose of this article is to explore the mechanisms of this and kindred ‘importing notions’ by investigating the role that the notion of science and proven experience plays in Swedish law and in the transfer of information between the legal and medical fields.
Using psychometric data from the stated preference (SP) experiments to search explanatory power for appropriateness of congestion charging policy
Authors: Saleh Sofyan M. , Sugiarto Sugiarto , Mutiawati Cut , Angraini Renni , Isya Muhammad 
Published: February 2017

Comprehensive stated preference (SP) experiment was conducted in Jakarta (a capital of Indonesia), where proposal for congestion charge (CC) has been considered as a way to reduce acute traffic congestion. With the government planning a CC scheme, public support is regarded as a prerequisite for its implementation. Therefore, a framework of structural equation model (SEM) is used to search explanatory power for the appropriateness of CC considering unobserved variable (latent variable) from psychometric data obtained from SP questionare. Causal paths among psychological determinants and their strength are measured and analyzed along with proposal acceptability from a psychological perspective. The findings from analysis with a SEM approach shows that a number of psychological determinants provide an explanation for the acceptability of the proposed scheme. The findings from analysis with a SEM approach shows that a number of psychological determinants provide an explanation for the appropriateness of the proposed scheme. Latent variables representing the validity of the CC scheme, such as ACE, APC and REC appear to have a significant explanation. These emerge as psychological determinants contributing a positive correlation with enhancement of appropriateness CC policy. Empirical result further shows that males have positive scores for the latent variables of car dependency (CDC) and inhibition freedom of movement (IFM). Furthermore, the variable of annual income, it has a positive correlation with recognition of the effects of CC in mitigating congestion and environmental problems (REC), car dependency (CDC) and awareness of the problems of cars in society (APC). This means that respondents with higher incomes are more concerned with the problems manifested by motorization while, on the contrary, the path coefficient between annual income (AI) and car dependency (CDC) has a value of 0.270. This discloses an automobile dependency. These findings should provide insight that designing a more acceptable policy in respecting to the acceptance of public in large.
Corporeal translation across borders: Indigenous choreographer Bulareyaung Pagarlava and his Warriors
Authors: Yatin Lin 
Published: February 2017

Abstract
This article looks into a genealogy of the multiple versions of the work Warriors (2010–2014) choreographed by Bulareyaung Pagarlava (also known as ‘Bula’). I discuss the evolution of the work alongside the search for his indigenous identity over the past few years as a freelance choreographer after having created works for dance companies from Taiwan such as Cloud Gate 2 and LAFA & Artists, then collaborating with performers from various institutions such as his alma mater the Taipei National University of the Arts (TNUA), the Martha Graham Dance Company, and the American Dance Festival (ADF), as well as the Formosa Aboriginal Song and Dance Troupe from Taiwan, leading up to the founding of his own Bulareyaung Dance Company (BDC) in 2014. Borrowing from Japanese cultural theorist Naoki Sakai’s concepts of subjectivity and translation, I trace how Bulareyaung gradually developed his indigenous creative voice after years of working with dancers of different ethnicities and nationalities from around the world, and how he chose to return home and set up an ensemble in his rural hometown of Taitung in southeastern Taiwan, gradually developing new movement vocabularies in tune with his indigenous subjects.
Selected Technical Solutions in Construction for Elderly People in Poland
Authors: Nicał A. K. 
Published: February 2017

AbstractThis paper brings up the issue of population aging and its influence on designing and construction of buildings in Poland. It also touches the problem of adaptation of existing facilities to the needs of the elderly and the disabled. Moreover, current trends in Polish demographics and possible outlook for the next 45 years has been presented. The author presents the current legal state concerning rules and regulations on the aspects of adjusting the buildings and their parts to the needs of the disabled. Additionally, the dimensions and the areas of movement for the disabled as well as the primary functional and requirements for bathrooms have been described. In the next paragraph, a concept of Ambient Assisted Living with examples of sanitary equipment for use in residential buildings has been propose.
The Statistical Analysis of Relation between Compressive and Tensile/Flexural Strength of High Performance Concrete
Authors: Kępniak M. , Woyciechowski P. 
Published: February 2017

AbstractThis paper addresses the tensile and flexural strength of HPC (high performance concrete). The aim of the paper is to analyse the efficiency of models proposed in different codes. In particular, three design procedures from: the ACI 318 [1], Eurocode 2 [2] and the Model Code 2010 [3] are considered. The associations between design tensile strength of concrete obtained from these three codes and compressive strength are compared with experimental results of tensile strength and flexural strength by statistical tools. Experimental results of tensile strength were obtained in the splitting test. Based on this comparison, conclusions are drawn according to the fit between the design methods and the test data. The comparison shows that tensile strength and flexural strength of HPC depend on more influential factors and not only compressive strength.
Scaling Resistance and Air Void Characteristics in Concrete Containing GGBS
Authors: Wawrzeńczyk J. , Molendowska A. , Juszczak T. 
Published: February 2017

AbstractIn this paper we discuss the test results for concretes containing various amounts of ggbs as compared to concretes made with Portland cement. The main objective of these tests is to evaluate the influence of varying air content in such mixtures on the structure and frost resistance of concrete. The authors suggest that the approach presented here allows for a safe design of concrete mixtures in terms of their frost resistance.The results indicate that concrete can be resistant to surface scaling even at the W/C ratio markedly higher than 0.45. Increased addition of ggbs leads to a decrease in concrete resistance to surface scaling. Proper air entrainment is the fundamental factor for frost-resistant concrete, and the air void system has to be assessed (micropore content A300, spacing factor $\overline L $). The addition of ggbs increases pore diameters, thus, to obtain the appropriate air pore spacing factor, micropore quantities introduced have to be increased.
Indignation as a political dynamics category
Authors: Brázda Radim 
Published: February 2017

Abstract
T. H. Macho defended the claim that politics is a system for organizing attention and for arranging relationships of visibility. One way of attracting and holding the attention of others and maintaining one’s visibility is the instrumentalization of indignation. Another way is to instigate and maintain social stress and unrest. The article explores the concepts of indignation and social stress as introduced by P. Sloterdijk. These concepts are part of a model of political dynamics that describes 1) the relationship between indignation and stress in political super-units and 2) the fact that they are compensated for by means of a specific conception of individual freedom (eleuthería, J.-J. Rousseau) and a liberal social order. The article defends the claim that there is a relationship between Macho’s claim about the organization of attention, Sloterdijk’s analyses of the concept of freedom and the method whereby individuals are immunized against generated social stress, which maintains social cohesion.
A Class of Exact Solutions of (3+1)-Dimensional Generalized B-Type Kadomtsev–Petviashvili Equation
Authors: Liu Shuang , Ding Yao , Liu Jian-Guo 
Published: February 2017

Abstract
By employing the generalized

(

G
′

/

G
)

$(G'/G)$

-expansion method and symbolic computation, we obtain new exact solutions of the (3 + 1) dimensional generalized B-type Kadomtsev–Petviashvili equation, which include the traveling wave exact solutions and the non-traveling wave exact solutions showed by the hyperbolic function and the trigonometric function. Meanwhile, some interesting physics structure are discussed.
Official Commemoration of the NATO Bombing of Serbia. A Case Study of the Fifteenth Anniversary
Authors: Mandić Marija 
Published: February 2017

Abstract
This article analyses how NATO’s bombing of Serbia has been officially commemorated in that country. Initially, it provides an overview of the commemorations performed between 2000 and 2013, covering both the commemorative practices and policies of leading Serbian politicians and alternative voices. The focus then turns to the fifteenth anniversary of the bombing in 2014. Just as in previous commemorations, there was no central ceremony, but, rather, a series of commemorative events held all over the country. The controversies that these aroused are then discussed, in particular those surrounding the commemoration of Radio Television Serbia’s employees and the spontaneous commemorative acts that took place in Serbian schools.
‘The Song Has Kept Us’: Soundscape of Belgrade during the NATO Bombing
Authors: Atanasovski Srđan 
Published: February 2017

Abstract
In this article I explore the sonic and music practices in the experience of the NATO bombing of Belgrade, focusing particularly on their role in the governmental apparatuses both of the NATO forces and of Miloševic’s regime. Drawing on affect studies, I discuss sound and music not only as text, but as sheer intensity, as a vibrational body and force. I argue that the sonic element of the experience of NATO bombing proved important as it provided the surface area, the somatic layer of the war machine, on which the apparatuses of governance could operate and effectuate the production of meaning.
Agents of Change. Women’s Advocacy during Democratization in Croatia
Authors: Rošul-Gajić Jagoda 
Published: February 2017

Abstract
This article investigates how Croatian women’s NGOs have contributed to gender policy in Croatia and what instruments they have used at the state level in drafting, initiating, and adopting innovative gender policy. It argues that, as norm advocates, Croatian women’s NGOs have adopted a double strategy: first, calling attention to the Croatian government’s non-compliance with international norms on women’s rights and second, enforcing change. Based on the advocacy of Croatian women’s NGOs, the author introduces the double-strategy model of norm implementation. The methodology is descriptive, whereas the analysis is based both on interviews and on published secondary data.
Experimental Nerium oleander poisoning in Balb/c mice and Wistar rat: comparative hepatotoxicity and nephrotoxicity effects based on biochemical and pathological studies
Authors: Khordadmehr Monire , Nazifi Saeed , Mansourian Maryam , Basiri Sara , Kolahian Saeed 
Published: February 2017

Abstract

Objective

Nerium oleander is a member of the Apocynaceae family. All parts of the plant are considered toxic and can poison livestock and humans.

Method
The present paper was carried out to compare the toxic effect of oral administration of N. oleander extract at single doses of 10, 12.5, 15 and 25 mg/kg body weight in Balb/c mice and Wistar rat. The toxicity of this plant was determined by measuring serum levels of ALT, AST, total protein, albumin, BUN and creatinine. Histopathological examination was performed on the liver and kidney.

Results
Significant differences were observed in the level of the ALT, AST, BUN and creatinine. Interestingly, the biochemical changes were more severe in AST in rats compared with mice (15–16 and 4–5 times compared to control, respectively). In comparison, the values of BUN in rats were higher compared with mice (2–2.5 and 1–1.5 times, respectively). In mice and in rat more sever toxic lesions were observed in the liver and kidney, respectively.

Conclusion
In conclusion, the biochemical and pathological results of the current study suggested that mice have more susceptibility to hepatotoxicity of N. oleander intoxication. But, rats show more susceptibility to nephrotoxicity of N. oleander poisoning.
Viscoelastic and Mechanical Properties of Polyimide-Clay Nanocomposites
Authors: Abdalla Mohamed O. , Dean Derrick , Campbell Sandi 
Published: February 2017

AbstractPolyimide-clay nanocomposites were prepared by blending 2.5 and 5 wt.% of PGV (Na+-montmorillonite) and two organically modified PGV (PGVC10COOH, PGVC12) with a methanol solution of PMR-15 precursor. DMA results showed a significant increase in the thermomechanical properties (E' and E'') of 2.5 wt.% clay loaded nanocomposites in comparison with the neat polyimide. Increasing the clay loading to 5 wt.% degraded these properties. Higher Tgs were observed for 2.5 wt.% nanocomposites compared to the neat polyimide. A lower CTE was observed only for the PGV/PMR-15 nanocomposite. Flexural properties measurements for the 2.5 wt.% nanocomposites showed an improvement in the modulus, strength and elongation. This trend in the tensile properties was not observed for the 5 wt.% nanocomposites.