bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analgesic activity of a new cannabinoid CB1 receptor modulator
Authors: Gurkin N. V. , Bykov V. V. , Bykova A. V. , Motov V. S. , Larchenko V. V. , Il’ina I. V. , Khazanov V. A. , Volcho K. P. , Salakhutdinov N. F. , Vengerovskii A. I. 
Published: October 2024

Aim. To study the analgesic activity, the effect on motor functions, and the potential ulcerogenic effect of a new 2H-chromene derivative, a cannabinoid CB1 receptor modulator (code name – CHR).Materials and methods. The analgesic activity of the CHR compound was studied when injected intragastrically at an effective dose of 5 mg / kg in mouse models of acute chemogenic pain (formalin test), acute visceral pain (the acetic acid-induced writhing test), and thermal nociception (hot plate test and tail-flick test). It was compared to the effect of tramadol and morphine or diclofenac sodium at doses of 20, 4 or 10 mg / kg, respectively. The effect of a single intragastric injection of the CHR compound at a dose of 5 mg / kg on motor activity was evaluated in the open field test. The potential ulcerogenic effect of the CHR compound at a dose of 5 mg / kg with repeated intragastric administration was compared with the effect of diclofenac sodium at a dose of 10 mg / kg.Results. With subplantar administration of formalin to mice, the 2H-chromene derivative reduced the number of pain reactions by 43–63% (p < 0.05). With intraperitoneal administration of acetic acid to mice, it reduced the number of writhing responses by 50% and had the same analgesic effect as diclofenac sodium and tramadol. In the hot plate test, the CHR compound increased the latency time to painful stimuli by 34% (p < 0.05). In the tailflick test, it increased the latency time to painful thermal sensations by 32% (p < 0.05). The CHR compound at an effective dose of 5 mg / kg did not change the motor activity of mice in the open field test and did not cause the formation of erosions and ulcers in the gastric mucosa when administered repeatedly to rats.Conclusion. The 2H-chromene derivative CHR at an effective dose of 5 mg / kg has a pronounced analgesic effect in mouse models of chemogenic, visceral, and thermal pain, which is as strong as that of tramadol, morphine, and diclofenac sodium used at effective doses. The CHR compound at an effective dose does not inhibit motor functions and does not have an ulcerogenic effect.
Cardioprotective effect of lithium ascorbate in an in vivo model of myocardial infarction
Authors: Plotnikov E. V. , Chernov V. I. , Mukhomedzyanov A. V. , Maslov L. N. , Yusubov M. S. , Larkina M. S. , Artamonov A. A. , Belousov M. V. 
Published: October 2024

The aim of the work was to study the cardioprotective effect of lithium ascorbate in an in vivo model of myocardial infarction. In the course of the study, we searched for compounds promising for therapy of acute myocardial infarction.Materials and methods. Myocardial infarction was modeled in Wistar rats by ligating the left coronary artery (the duration of ischemia was 45 minutes) followed by ligature loosening and 120-minute reperfusion. All manipulations were performed under alpha-chloralose anesthesia with mechanical lung ventilation and recording heart rate, blood pressure, and ECG. Lithium ascorbate was administered intravenously at a dose of 100 mg / ml before ischemia. The area at risk (the ischemia / reperfusion zone) was detected by staining the myocardium with tightened ligature with 5% potassium permanganate. After that consecutive myocardial slices were prepared, and infarct size was determined. Differentiation of the infarct size from the area at risk was performed by staining with 1% 2,3,5-triphenyl tetrazolium chloride solution for 30 minutes at 37 ºC. The infarct size and the area at risk were determined by the planimetric method. The serum concentration of myocardial damage marker creatine kinase-MB (CK-MB) was measured using ELISA kits.Results. Lithium ascorbate reduced the infarct size / area at risk ratio by 38% and decreased the serum CPKMB level in the experimental animals by 42% compared to the control group. Lithium ascorbate did not affect hemodynamics parameters during coronary artery occlusion and reperfusion.Conclusion. The cardioprotective effect of lithium ascorbate in cardiac ischemia / reperfusion in vivo was found.
The effect of a new 3-hydroxypyridine derivative LHT-2-20 on free radical oxidation in experimental periodontitis
Authors: Poryadin G. V. , Zakhvatov A. N. , Yasnetsov V. V. , Skachilova S. Ya. , Khaydar D. A. , Tarasova T. V. , Zakharkin I. A. , Parshina A. Yu. , Simakina E. A. 
Published: October 2024

Aim. To study the effect of a new complex compound LHT-2-20 (2-ethyl-6-methyl-3-hydroxypyridine-2-(3benzoyl phenyl)-propanoate) on free radical oxidation in experimental periodontitis.Materials and methods. The experimental study was performed on 195 white mongrel mice weighing 19–23 g and 137 white mongrel rats weighing 180–220 g. The effect of a new complex compound LHT-2-20 (2-ethyl-6methyl-3-hydroxypyridine-2-(3-benzoyl phenyl)-propanoate) on the intensity of free radical oxidation and the local state of periodontal tissues during a course of intragastric administration was studied on the experimental model of periodontitis. Statistical processing of the results was carried out using the SPSS Statistics 20.0 software package with the analysis of variance (ANOVA) and the parametric Tukey’s test.Results. The LHT-2-20 compound reduced elevated levels of primary and secondary lipoperoxidation products (conjugated dienes, malondialdehyde in plasma and in erythrocytes during spontaneous and iron-induced oxidation) already at the early stages of the experiment, bringing the studied parameters closer to the reference values by the end of the course of treatment. The use of the compound LHT-2-20 contributed to an increase in the activity of the main antioxidant enzymes (catalase and superoxide dismutase), normalizing them to baseline values by the end of the experiment. With the correction of free radical processes, the use of LHT-2-20 limited the local inflammatory response in periodontal tissues, which was confirmed by a decrease in gingival edema and hyperemia, bleeding, depth of periodontal pockets, and tooth mobility.Conclusion. The results of this study confirm the anti-inflammatory potential of the compound and the multiplicity of its effects due to the impact on the mechanisms of oxidative stress. The expediency of further study of the drug is justified by the prospect of creating a new drug and its subsequent wide clinical application as part of the complex therapy of periodontal inflammation.
Beta-adrenergic reactivity of erythrocyte membranes in adolescents with supraventricular and ventricular arrhythmias before and after radiofrequency ablation
Authors: Rebrova Т. Yu. , Perevoznikova Yu. E. , Muslimova E. F. , Svintsova L. I. , Afanasiev S. A. , Dzhaffarova O. Yu. 
Published: October 2024

Aim. To evaluate β-adrenergic reactivity of erythrocyte membranes (β-ARM) in adolescents with ventricular and supraventricular arrhythmias before and after radiofrequency ablation (RFA) of heart rhythm disturbances.Materials and methods. The study included 49 adolescents aged 11–17 years, of which 15 had Wolff – Parkinson – White pattern (WPW), 13 – WPW syndrome, 10 – atrioventricular nodal reentry tachycardia (AVNRT), and 11 – ventricular arrhythmia (VA). The control group consisted of 11 adolescents without cardiovascular pathology. All patients received surgical treatment for heart rhythm disturbances (HRD) using RFA. In patients with HRD, β-ARM was determined by a set of reagents BETA-ARM AGAT (AGAT LLC, Russia) before RFA and 3 days after it. In the control group, the parameter was determined at the stage of inclusion in the study.Results. In adolescents with supraventricular arrhythmias, median values of β-ARM did not differ significantly from the control group. RFA in adolescents in these groups did not affect the value of β-ARM on day 3 after the surgery. In adolescents with VA, the median value of β-ARM was initially higher than in the control group (p = 0.026). On day 3 after RFA, an increase in β-ARM was noted in this group (p = 0.028) compared to baseline values.Conclusion. Activation of the sympathetic nervous system plays a significant role in the pathogenesis of VA in adolescence. The study showed the possibility of using β-ARM to assess the state of the sympathetic nervous system in patients with methodological limitations in analyzing heart rate variability.
Characterization of Different Mango (Mangifera indica) Varieties of South Punjab
Authors: Shahid Amna , Ikhlaq Muhammad , Noreen Ammara , Amjad Muhammad Ammar , Bashir Muhammad Azhar , Rasool Fatima , Latif Amna 
Published: October 2024

One of the great and popular fruit produces in tropical and subtropical areas of the world is the mango (Mangifera indica Linn.), stated as the &quot;King of Fruits,&quot;. Varietal description is a vital constituent of mango enhancement and breeding. Objective of this study was choosing important morphological and biochemical indicators for mangos to serve as future reference points for varietal identification and breeding efforts was another objective. These varieties include Anwar Ratol, Azeem Chaunsa, Alishan, Dusehri, Haiden. The results of this investigation support the hypothesis that there is substantial variance among genotypes depending on physical and biochemical attributes. South Punjab has a varied germplasm that can be castoff for crossbreeding, and effective exploration will be carried out to fully realize the probable. Description is therefore a crucial prerequisite for initiating a breeding effort.
Patient-Level Value Analysis in Total Hip Arthroplasty: Optimizing the Value of Care Delivery
Authors: Doxey Stephen A. , Huyke-Hernández Fernando A. , Davidson Samuel , Kleinsmith Rebekah M. , Qian Lily J. , Krohn Eric J. , Horst Patrick K. , Bingham Joshua S. , Cunningham Brian P. 
Published: October 2024

Background:
Value is defined as the ratio of patient outcomes to the cost of care. One method to assess value is through patient-level value analysis (PLVA). To our knowledge, this tool has not previously been implemented in the setting of total hip arthroplasty (THA). The purposes of this study were to perform PLVA for a 1-year episode of care among patients undergoing primary THA and to identify characteristics that affect value in a metropolitan health-care system.

Methods:
The patient-reported outcome (PRO) measure database of the institution was queried for all primary THAs from 2018 to 2019. The PRO measure that was utilized was the Hip disability and Osteoarthritis Outcome Score, Joint Replacement (HOOS-JR). Cost was evaluated with use of time-driven activity-based costing (TDABC) for a 1-year episode of care (consisting of the day of surgery, inpatient stay, discharge facility, postoperative appointments, and physical therapy). The primary outcome was the 1-year value quotient, or the ratio of the 1-year change in HOOS-JR to the cost of the episode of care (VHOOS). The value quotient was compared among all included patients and evaluated for variables that may affect the overall value of the episode of care.

Results:
In total, 480 patients were included in the analysis. The mean improvement in the HOOS-JR was +34.9 ± 16.1 (95% confidence interval [CI]: 33.5, 36.3). The mean cost was $13,835 ± $3,471 (95% CI: $13,524, $14,146). The largest contributor to cost was implants (39.0%), followed by post-recovery care (i.e., inpatient stay and specialized nursing facilities; 24.1%). Change in the HOOS-JR was poorly correlated with the cost of care (r = −0.06; p = 0.19). THAs performed at an ambulatory surgery center (ASC) with discharge to home demonstrated higher value (VHOOS = 0.42) than THAs performed at a hospital with discharge to a rehabilitation facility (VHOOS = 0.15; analysis of variance F-test, p < 0.01).

Conclusions:
Our study found that PROs did not correlate with costs in the setting of primary THA. Implants were the largest cost driver. Surgical setting (an ASC versus a hospital) and discharge destination influenced value as well. PLVA is a value measurement tool that can be utilized to optimize components of the care delivery pathway.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Identifying Risk Factors for Disease Progression in Developmental Dysplasia of the Hip Using a Contralateral Hip Model
Authors: Harris Michael D. , Thapa Susan , Lieberman Elizabeth G. , Pascual-Garrido Cecilia , Abu-Amer Wahid , Nepple Jeffrey J. , Clohisy John C. 
Published: October 2024

Background:
Developmental dysplasia of the hip (DDH) can cause pain and premature osteoarthritis. The risk factors and timing for disease progression in adolescents and young adults have not been fully defined. This study aimed to determine the prevalence of and risk factors for contralateral hip pain and surgery after periacetabular osteotomy (PAO) on a dysplastic hip.

Methods:
Patients undergoing unilateral PAO for DDH were followed for at least 2 years and categorized into contralateral pain and no-pain groups and contralateral surgery and no-surgery groups. Pain was defined with the modified Harris hip score. Univariate analysis tested group differences in demographics, radiographic measures, and range of motion. Kaplan-Meier survival analysis was used to assess pain development and surgery in the contralateral hip over time. Multivariable regression identified risk factors for contralateral pain and surgery. Contralateral pain and surgery predictors were secondarily assessed after categorization of the contralateral hips as dysplastic, borderline, and non-dysplastic and in subgroups based on the lateral center-edge angle (LCEA) and acetabular inclination (AI) in 5° increments.

Results:
One hundred and eighty-four patients were followed for a mean of 4.6 ± 1.6 years (range, 2.0 to 8.8 years), during which 51% (93) reported contralateral hip pain and 33% (60) underwent contralateral surgery. Kaplan-Meier analysis predicted 5-year survivorship of 49% with contralateral pain development as the end point and 66% with contralateral surgery as the end point. Painful hips exhibited more severe dysplasia compared with no-pain hips (LCEA = 16.5° versus 20.3°, p < 0.001; AI = 13.2° versus 10.0°, p < 0.001). AI was the sole predictor of pain, with every 1° increase in the AI raising the risk by 11%. Surgically treated hips also had more severe dysplasia (LCEA = 14.9° versus 20.0°, p < 0.001; AI = 14.7° versus 10.2°, p < 0.001) and were in younger patients (21.6 versus 24.1 years, p = 0.022). AI and a maximum alpha angle of ≥55° were predictors of contralateral surgery.

Conclusions:
At 5 years after hip PAO, approximately 50% of contralateral hips will have pain and approximately 35% can be expected to need surgery. More severe dysplasia, based on the LCEA and AI, increases the risk of contralateral hip pain and surgery, with AI being a predictor of both outcomes. Knowing these risks can inform patient counseling and treatment planning.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Genome sequence of the Mediterranean red coral Corallium rubrum
Authors: Ganot Philippe , Rausch Tobias , Fritz Markus , Zoccola Didier , Wang Xin , Aranda Manuel , Benes Vladimir , Allemand Denis , Tambutté Sylvie 
Published: October 2024

Abstract

Objectives: Corallium rubrum, the precious red coral, is an octocoral endemic to the western Mediterranean Sea. Like most octocorals, it produces tiny, calcified structures called sclerites. Uniquely, it also produces a completely calcified axial skeleton that is a bright red color. This combination of color and hardness has made the red coral prized for centuries, leading to extensive fishing and trade for use in jewelry. Understanding how it produces this red skeleton is thus a central question in economics, culture, and biology. To gain insights into this process, we sequenced the C. rubrum genome.Data description: Our C. rubrum genome assembly is 655 megabases (Mb) in size, distributed across 2,910 scaffolds with a very low level of unknown nucleotides (0.95%). We used a pipeline based on the MaSuRCA hybrid assembler, combining long PacBio reads and short Illumina reads, followed by several steps to improve the assembly, including scaffolding, merging, and polishing. This represents the third published genome of an octocoral and the first within the order Scleralcyonacea.
Extra-cranial metastases of Glioblastoma Multiform: cases reports and a review of the literature
Authors: Bravo-Gamboa Brian , Floyd-Aristizábal Danielle , Enríquez-Marulanda Alejandro , Escobar Luis Alberto , Rojas Oscar , Lobato-Polo Javier 
Published: October 2024

Abstract

Introduction: Glioblastoma multiforme (GBM) is the most common and aggressive malignant glioma. Despite the well-known invasiveness in the central nervous system (CNS), yet they are considered to be typically confined because extra-cranial metastasis of glioblastoma rarely occurs.
Case presentation: We describe two cases, the first is about a 58-year-old woman with a diagnosis with GBM, which was subsequently taken to surgery with total resection and progress with recurrence in the temporal lobe. finally presented a spinal cord metastasis. the second case of a fifteen-year old patient who initially presented with a low-grade glioma, which was managed with surgical resection, radiation therapy and a ventriculoperitoneal shunt because of obstructive hydrocephalus. Despite of the treatment, the tumor progressed to GBM, associated with peritoneal metastasis.
Conclusion: Glioblastoma extracranial disease is rare, and reporting cases could help to collect data that might orient about mechanism of dissemination, and develop future investigations on this topic.
Pulmonary involvement in inflammatory bowel diseases: report of a national cohort over the past 5 years
Authors: Papazian L. , Giovannini-Chami L. , Hadchouel A. , Pouessel G. , Gilardoni J.M. , Thumerelle C. , Renoux MC. , Fabre A. , Dubus JC. , Mazenq J. 
Published: October 2024

Abstract

Background
Chronic inflammatory bowel disease (IBD) is a common illness, with a number of cases constantly rising. Pulmonary involvement is rarely described in children with IBD. This national survey aims to describe the clinical, biological and therapeutic data of IBD children with pulmonary involvement followed from January 2017 to April 2023 in a rare respiratory diseases centre.
Methods
This was a retrospective, observational, descriptive, multicentre study conducted within the French RespiRare network. Socio-demographic characteristics, previous events, clinical and paraclinical data, treatment and evolution were collected via an anonymous secure questionnaire.
Results
Seven IBD children with pulmonary involvement were reported (sex ratio 0.75, median age 10 years, 4 with Crohn disease and 3 with ulcerative colitis). For 2 patients, the discovery of pulmonary involvement was concomitant with the diagnosis of Crohn's disease. Pulmonary symptoms were non-specific or absent. Chest computed tomography scans revealed condensation (n = 5), mosaic appearance (n = 2), excavated (n = 2) or non-excavated (n = 3) nodules or micronodules. Bronchoalveolar lavage revealed hypercellularity with hyperlymphocytosis in 4 patients among 6. Biopsies (n = 5) showed inflammation or granulomas. Organized pneumonia was diagnosed in 5 patients, granulomatosis in 1 and necrotizing pneumonia in 1. Most patients were treated with oral corticosteroids, with a favourable outcome.
Conclusions
This is the first national reported cohort of IBD children with pulmonary involvement. They are discussed in regard to the 29 previous published cases we found in the same period.
Comparison of the efficacy of esophageal balloon dilatation and Botox injection, with esophageal balloon dilatation alone, in children with achalasia referred to Mofid children hospital (2020-2023)
Authors: Dara Naghi , Aghaei Mohammad , Hosseini Amirhossein , zarinfar yasaman , Dara Arshia , Sayyari Aliakbar , Olang Beheshteh , Khalili Mitra , Imanzadeh Farid , hajipour Mahmoud 
Published: October 2024

Abstract

Background: Achalasia is an esophageal motility disorder with symptoms like regurgitation, dysphagia, anorexia, and chronic cough. Effective treatments include pneumatic dilatation and myotomy. This study evaluated the combined use of botulinum toxin (Botox) injection and esophageal balloon dilation versus balloon dilation alone, analyzing recovery rates for gastrointestinal and non-gastrointestinal symptoms.
Study method: This clinical trial involved an intervention group and historical controls. Patients with symptomatic achalasia referred to Mofid Hospital (2020-2023) received balloon dilatation with Botox injection. Historical controls had balloon dilatation alone. Botox was injected at four points in the Lower Esophageal Sphincter (LES) before balloon dilatation. Symptoms and growth parameters were monitored every three months for a year, then every six months, with annual follow-ups for three years. The study included 37 intervention and 31 control patients, and data were analyzed using SPSS 24 software.
Results: From the onset of the study to the 36th month, the intervention group showed greater symptom improvement than the control group: nausea or vomiting (19%), regurgitation (21%), dysphagia to liquids (37%), dysphagia to solids (10%), globus sensation (10%), anorexia (2.14%), weight loss (18%), halitosis (15%), cough (44%), aspiration (18%), and refusal to eat (7%). Exceptions were chest pain (same in both groups) and heartburn (14% better in the control group). At 6 and 12 months, the intervention group improved in abdominal tenderness (4.2%), wheezing (7.11%), and coarse rales (20.9%). Radiological signs improved more in the intervention group: esophageal dilatation (11.6%), rat tail/bird beak sign (31.7%), tertiary contractions (10.4%), and reduced air-fluid level in the esophagus (upper to middle third: 17%, middle to lower third: 35.8%), except for the sigmoid esophagus (same in both groups). At 36 months, growth criteria in the intervention group increased: weight (4 kg), length (5.35 cm), BMI (1.47 units), Z score (0.65 units), and percentile (5.65 units).
Conclusion: The combined balloon and Botox treatment has shown effectiveness in improving disease symptoms, physical examination results, growth criteria, and radiological findings. Further studies are recommended to potentially establish this approach as a national protocol.
Does Auto-clave Sterilization Affect Nickle Ion Release of Stainless Steel Crowns and Space Maintainer Bands?
Authors: Alessa Noura , Alhamdan Habah , Alrobaish Shahad , AlQahtani Nisrin , Alarifi Norah , Almutairi Manal , Alhowaish Latifa , Al-Homaidhi Majedah 
Published: October 2024

Abstract

Aim
This study aimed to investigate the effect of autoclave sterilization on the leaching of nickel ions from stainless steel crowns and space-maintainer bands used in pediatric dentistry.
Materials and Methods
Sixty space maintainer bands and 60 stainless steel crowns were divided into sterilized and non-sterilized groups. These samples were immersed in artificial saliva, placed in individual petri dishes, and then incubated at 37°C. The samples were analyzed for 42 d using a gas chromatography-mass spectrometry (GC-MS) machine to quantify the release of nickel ions.
Results
Nickel release was higher in the sterilized samples than in the non-sterilized samples for both space maintainer bands and stainless steel crowns. This trend persisted throughout most days of the experiment, with a peak release observed on day 7. The sterilized group showed significantly higher levels of ion release than the non-sterilized group (p < 0.05).
Conclusion
This study suggests that nickel allergy testing may be advisable before placing stainless steel crowns or space maintainers, particularly for patients at risk of allergies. Dentists should be mindful of the increased nickel release observed from sterilized crowns and bands, as this information could influence treatment decisions, especially in children who are predisposed to allergies.