bluuebunny/crossref_metadata_2025_split huggingface dataset:
BMP8B Is a Tumor Suppressor Gene Regulated by Histone Acetylation in Gastric Cancer
Authors: Wisnieski Fernanda , Leal Mariana Ferreira , Calcagno Danielle Queiroz , Santos Leonardo Caires , Gigek Carolina Oliveira , Chen Elizabeth Suchi , Artigiani Ricardo , Demachki Sâmia , Assumpção Paulo Pimentel , Lourenço Laércio Gomes , Burbano Rommel Rodríguez , Smith Marília Cardoso 
Published: October 2016

ABSTRACTDifferent from genetic alterations, the reversible nature of epigenetic modifications provides an interesting opportunity for the development of clinically relevant therapeutics in different tumors. In this study, we aimed to screen and validate candidate genes regulated by the epigenetic marker associated with transcriptional activation, histone acetylation, in gastric cancer (GC). We first compared gene expression profile of trichostatin A‐treated and control GC cell lines using microarray assay. Among the 55 differentially expressed genes identified in this analysis, we chose the up‐regulated genes BMP8B and BAMBI for further analyses, that included mRNA and histone acetylation quantification in paired GC and nontumor tissue samples. BMP8B expression was reduced in GC compared to nontumor samples (P < 0.01). In addition, reduced BMP8B expression was associated with poorly differentiated GC (P = 0.02). No differences or histopathological associations were identified concerning BAMBI expression. Furthermore, acetylated H3K9 and H4K16 levels at BMP8B were increased in GC compared to nontumors (P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC (P < 0.05). Reduced levels of acetylated H3K9 was also associated with diffuse‐type histological GC (P < 0.05). Notably, reduced BMP8B mRNA and acetylated H4K16 levels were positively correlated in poorly differentiated GC (P < 0.05). Our study demonstrated that BMP8B seems to be a tumor suppressor gene regulated by H4K16 acetylation in poorly differentiated GC. Therefore, BMP8B may be a potential target for TSA‐based therapies in this GC sample subset. J. Cell. Biochem. 118: 869–877, 2017. © 2016 Wiley Periodicals, Inc.
Grape yield and quality responses to simulated year 2100 expected climatic conditions under different soil textures
Authors: Leibar Urtzi , Pascual Inmaculada , Morales Fermín , Aizpurua Ana , Unamunzaga Olatz 
Published: October 2016

AbstractBACKGROUNDThe influence of global warming on grape quality is a great concern among grapegrowers and enologists. The effects of simulated year 2100 expected CO2, temperature and relative humidity (RH) conditions (FCC; 700 µmol CO2 mol−1 air, 28/18 °C day/night and 33/53% RH, day/night) versus the current situation (Curr; 390 µmol CO2 mol−1 air, 24/14 °C and 45/65% RH); well‐irrigated versus expected future water deficit and three soils with different clay contents (41, 19 and 8%) on yield and berry quality of grapevine cv. Tempranillo were evaluated.RESULTSFCC shortened the time between fruit set and veraison and between fruit set and maturity by up to 7 and 10 days, respectively. This faster maturity led to higher must pH and tonality and reduced malic and tartaric acid concentrations, total anthocyanin concentration and colour intensity. Water deficit delayed ripeness for up to 9 days and reduced vegetative growth and malic acid concentration of grapes. However, this malic acid reduction did not occur with the clayey soils. These soils induced the lowest root fresh weight and berries with lower total anthocyanin concentration.CONCLUSIONAmong the adaptation techniques to cope with the described effects on fruit composition, soil selection should be considered with attention in addition to irrigation practices. © 2016 Society of Chemical Industry
Effect of nursing or mentoring by adult cows on physical activity, performance and meat quality of fattening beef calves kept on alpine pastures
Authors: Gangnat Isabelle DM , Dufey Pierre‐Alain , Silacci Paolo , Messadene‐Chelali Jessika , Kreuzer Michael , Berard Joel 
Published: October 2016

AbstractBACKGROUNDCompared with weaned calves, nursed calves benefit from access to milk as nutrient‐dense feed and mentoring by their dams. Both may be decisive for growth under difficult conditions such as grazing on alpine pastures, where experience could play an essential role. These factors were investigated separately by comparing nursed calves with calves weaned at 3 months of age and grazing with or without a mentor cow (n = 8 calves).RESULTSWeaned calves with mentor cows spent less time lying and walked more than nursed calves. Irrespective of whether they had a mentor cow or not, the weaned calves grew slower than the nursed calves (357, 428 and 1324 g day−1 respectively). This also adversely affected slaughter weight, dressing percentage, conformation and fat cover at slaughter. In general, mentoring had no effect. Meat quality did not differ whether weaned calves were mentored or not. Nursed calves were superior in intramuscular fat content, tenderness and water‐holding capacity.CONCLUSIONThe availability of milk appeared to be much more important to the calves under demanding grazing conditions than did mentoring. The differences in growth rate were accompanied by unexpectedly large differences in meat quality, showing that early weaning was not suitable for this system.© 2016 Society of Chemical Industry
A fluorescence‐activated cell sorting subsystem for the Imaging FlowCytobot
Authors: Lambert Bennett S. , Olson Robert J. , Sosik Heidi M. 
Published: October 2016

AbstractRecent advances in plankton ecology have brought to light the importance of variability within populations and have suggested that cell‐to‐cell differences may influence ecosystem‐level processes such as species succession and bloom dynamics. Flow cytometric cell sorting has been used to capture individual plankton cells from natural water samples to investigate variability at the single cell level, but the crude taxonomic resolution afforded by the fluorescence and light scattering measurements of conventional flow cytometers necessitates sorting and analyzing many cells that may not be of interest. Addition of imaging to flow cytometry improves classification capability considerably: Imaging FlowCytobot, which has been deployed at the Martha's Vineyard Coastal Observatory since 2006, allows classification of many kinds of nano‐ and microplankton to the genus or even species level. We present in this paper a modified bench‐top Imaging FlowCytobot (IFCB‐Sorter) with the capability to sort both single cells and colonies of phytoplankton and microzooplankton from seawater samples. The cells (or subsets selected based on their images) can then be cultured for further manipulation or processed for analyses such as nucleic acid sequencing. The sorting is carried out in two steps: a fluorescence signal triggers imaging and diversion of the sample flow into a commercially available “catcher tube,” and then a solenoid‐based flow control system isolates each sorted cell along with 20 μL of fluid.
Gold Nanocarriers for Macrophage‐Targeted Therapy of Human Immunodeficiency Virus
Authors: Zazo Hinojal , Colino Clara I. , Warzecha Klaudia T. , Hoss Mareike , Gbureck Uwe , Trautwein Christian , Tacke Frank , Lanao José M. , Bartneck Matthias 
Published: October 2016

The human immunodeficiency virus (HIV) continues to be a global pandemic and there is an urgent need for innovative treatment. Immune cells represent a major target of virus infection, but are also therapeutic targets. Currently, no antiretroviral therapy targets macrophages, which function as portal of entry and as major long‐term deposit of HIV. It has been shown before that human macrophages efficiently internalize gold nanoparticles, a fact which might be used to target them with drug‐nanoparticle conjugates. Here, the authors use gold nanocarriers to facilitate delivery of stavudine, a widely used antiretroviral drug, to primary human macrophages. Using an ease‐of‐use coupling method, a striking potentiation of stavudine intake by macrophages using gold nanocarriers is shown. Further, the carriers induce a specific subtype of proinflammatory activation indicative for antiviral activity of macrophages, which suggests promising novel treatment options for HIV.image
Sequence Determination and Regulation in the Living Anionic Copolymerization of Styrene and 1,1‐Diphenylethylene (DPE) Derivatives
Authors: Ma Hongwei , Han Li , Li Yang 
Published: October 2016

The living anionic copolymerization of styrene (St) and 1,1‐diphenylethylene (DPE) derivatives were carried out to investigate how the DPE units are distributed along the polymer chain. Based on the combination of timing sample method and exact characterizations, the sequence determination method was established to reveal the sequential distribution of DPE units along the functionalized chains. In the copolymerization of St and DPE‐SiH, when DPE‐SiH was excessively fed, a strictly alternating structure can be prepared. As the feed ratios of S/D increased, a gradient structure was obtained. Additionally, when the substituent structure of the DPE derivatives changed, the sequence structure could be regulated. It has been confirmed in the copolymerization of St and DPE‐SiH/OMe or DPE‐SiH/NMe2, that the electron‐donating group (‐OMe or ‐NMe2) was introduced into DPE‐SiH. Then, the preliminary sequence regulation method was found. Meanwhile the sequence‐determined grafting was investigated, and bottlebrush polymers with sequence‐determined branches were successfully synthesized.
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Genetic analysis of the dystrophin gene in children with Duchenne and Becker muscular dystrophies
Authors: Zhong Jingzi , Xu Tiantian , Chen Gang , Liao Haixia , Zhang Jiapeng , Lan Dan 
Published: October 2016

ABSTRACTIntroductionDuchenne and Becker muscular dystrophies (DMD and BMD) are X‐linked myopathies caused by mutations of the dystrophin gene.MethodsMultiplex ligation‐dependent probe amplification (MLPA) combined with next‐generation sequencing (NGS) of the exons of the dystrophin gene were performed in 92 suspected DMD/BMD patients. Patients with negative results were subjected to additional muscle diseases panel tests.ResultsDNA rearrangements were detected in 65 (70.65%) patients using MLPA. The deletions primarily clustered at exons 45–55, followed by exons 2–19. The duplication locations were in contrast to previous studies, which involved the 3′ end of the gene. A total of 21 cases with point mutations were detected by NGS analysis. Furthermore, 6 previously unreported mutations were detected. Limb‐girdle muscular dystrophy was confirmed in 2 patients after analysis with the muscle diseases panel.ConclusionsMLPA combined with NGS was effective for detection of the mutations in dystrophin gene exons. Muscle Nerve 56: 117–121, 2017.
Hot Air or Comprehensive Progress? A Critical Assessment of the SDGs
Authors: Spangenberg Joachim H. 
Published: October 2016

AbstractSince the SDGs have been adopted, the focus of the policy, civil society and academic debate is on how to implement ‘the SDGs’. However, if there are inherent contradictions, it will be impossible to implement all goals, and a choice has to be made. This paper analyses the SDGs regarding their coherence and its impacts on their implementation. It does so by using the DPSIR heuristic looking at driving forces, pressures, states, impacts and responses of sustainability challenges. The opportunities for success are assessed by a closer look at the agency mentioned in the SDG–Agenda 2030 text. The SDGs are found to be weak on agency, with limited obligations to governments and none to business or consumers. They focus on state and impact, neglecting the pressures and supporting counterproductive drivers. In conclusion, either the positive targets will not be realized, or the means of implementation must be upgraded significantly. Copyright © 2016 John Wiley & Sons, Ltd and ERP Environment
DNA‐Decorated Luminescent Vesicles as Drug Carriers
Authors: Thelu Hari Veera Prasad , Albert Shine K. , Golla Murali , Krishnan Nithiyanandan , Yamijala Santosh Bhargav , Nair Sreeja V. , Srinivasula S. Murty , Varghese Reji 
Published: October 2016

AbstractDNA based spherical nanostructures is one of the promising nanocarriers for drug delivery applications because of their excellent biocompatibility and unique structural features of DNA. Herein, we report the amphiphilicity driven self‐assembly of DNA‐phenyleneethynylene based amphiphile into luminescent vesicles and their cellular uptake toward HeLa cell. Spectroscopic and microscopic analyses reveal the self‐assembly of this class of amphiphiles into vesicular nanostructures with corona made of DNA and a functional hydrophobic membrane. Vesicles exhibit promising cell permeability.
Healthy Change Processes—A Diary Study of Five Organizational Units. Establishing a Healthy Change Feedback Loop
Authors: Lien Mathilde , Saksvik Per Øystein 
Published: October 2016

AbstractThis paper explores a change process in the Central Norway Regional Health Authority that was brought about by the implementation of a new economics and logistics system. The purpose of this paper is to contribute to understanding of how employees' attitudes towards change develop over time and how attitudes differ between the five health trusts under this authority. In this paper, we argue that a process‐oriented focus through a longitudinal diary method, in addition to action research and feedback loops, will provide greater understanding of the evaluation of organizational change and interventions. This is explored through the assumption that different units will have different perspectives and attitudes towards the same intervention over time because of different contextual and time‐related factors. The diary method aims to capture the context, events, reflections and interactions when they occur and allows for a nuanced frame of reference for the different phases of the implementation process and how these phases are perceived by employees. Copyright © 2016 John Wiley & Sons, Ltd.
Transfer Hydrogenation in Water
Authors: Wu Xiaofeng , Wang Chao , Xiao Jianliang 
Published: October 2016

AbstractThis article provides an account of our group's efforts in developing aqueous‐phase transfer hydrogenation reactions. It is comprised of mainly two parts. The first part concentrates on asymmetric transfer hydrogenation in water, enabled by Noyori–Ikariya catalysts, while the second part is concerned with the achiral version of the reaction catalysed by a new class of catalysts, iridacycles. A range of substrates are featured, including various carbonyl compounds and N‐heterocycles.
Reproductive toxicity induced by nickel nanoparticles in Caenorhabditis elegans
Authors: Kong Lu , Gao Xiaojie , Zhu Jiaqian , Zhang Ting , Xue Yuying , Tang Meng 
Published: October 2016

ABSTRACTTo investigate the reproductive toxicity and underlying mechanism of nickel nanoparticles (Ni NPs), Caenorhabditis elegans (C. elegans) were treated with/without 1.0, 2.5, and 5.0 μg cm−2 of Ni NPs or nickel microparticles (Ni MPs). Generation time, fertilized egg numbers, spermatide activation and motility were detected. Results indicated, under the same treatment doses, that Ni NPs induced higher reproductive toxicity to C. elegans than Ni MPs. Reproductive toxicities observed in C. elegans included a decrease in brood size, fertilized egg and spermatide activation, but an increase in generation time and out‐of‐round spermatids. The reproductive toxicity of Ni NPs on C. elegans may be induced by oxidative stress. The reproductive toxicity in C. elegans induced by Ni NPs is consistent with our previous results in the rats. Therefore, C. elegans can be used as an alternative model to detect the early reproductive toxicity of Ni NPs exposure. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1530–1538, 2017.
Construction and Analysis of Functional Networks in the Gut Microbiome of Type 2 Diabetes Patients
Authors: Li Lianshuo , Wang Zicheng , He Peng , Ma Shining , Du Jie , Jiang Rui 
Published: October 2016

Abstract
Although networks of microbial species have been widely used in the analysis of 16S rRNA sequencing data of a microbiome, the construction and analysis of a complete microbial gene network are in general problematic because of the large number of microbial genes in metagenomics studies. To overcome this limitation, we propose to map microbial genes to functional units, including KEGG orthologous groups and the evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) orthologous groups, to enable the construction and analysis of a microbial functional network. We devised two statistical methods to infer pairwise relationships between microbial functional units based on a deep sequencing dataset of gut microbiome from type 2 diabetes (T2D) patients as well as healthy controls. Networks containing such functional units and their significant interactions were constructed subsequently. We conducted a variety of analyses of global properties, local properties, and functional modules in the resulting functional networks. Our data indicate that besides the observations consistent with the current knowledge, this study provides novel biological insights into the gut microbiome associated with T2D.
A pilot study exploring the molecular architecture of the tumor microenvironment in human prostate cancer using laser capture microdissection and reverse phase protein microarray
Authors: Pin Elisa , Stratton Steven , Belluco Claudio , Liotta Lance , Nagle Ray , Hodge K. Alex , Deng Jianghong , Dong Ting , Baldelli Elisa , Petricoin Emanuel , Pierobon Mariaelena 
Published: October 2016

AbstractThe cross‐talk between tumor epithelium and surrounding stromal/immune microenvironment is essential to sustain tumor growth and progression and provides new opportunities for the development of targeted treatments focused on disrupting the tumor ecology. Identification of novel approaches to study these interactions is of primary importance. Using laser capture microdissection (LCM) coupled with reverse phase protein microarray (RPPA) based protein signaling activation mapping we explored the molecular interconnection between tumor epithelium and surrounding stromal microenvironment in 18 prostate cancer (PCa) specimens. Four specimen‐matched cellular compartments (normal‐appearing epithelium and its adjacent stroma, and malignant epithelium and its adjacent stroma) were isolated for each case. The signaling network analysis of the four compartments unraveled a number of molecular mechanisms underlying the communication between tumor cells and stroma in the context of the tumor microenvironment. In particular, differential expression of inflammatory mediators like IL‐8 and IL‐10 by the stroma cells appeared to modulate specific cross‐talks between the tumor cells and surrounding microenvironment.
Do neighbourhoods in Vancouver and surrounding areas demonstrate different rates of bystander CPR and survival for out-of-hospital cardiac arrest?
Authors: Barbic David , Klinkenberg Brian , Grunau Brian , Christenson Jim 
Published: October 2016

AbstractObjectiveNo prior work exists examining the relation between the geographic distribution of out-of-hospital cardiac arrest (OHCA) in the city of Vancouver and surrounding areas that may exhibit a clustering of cases. The primary objective of this study was to describe the distribution of OHCA within the Vancouver Coastal Health region using a geographic information system (GIS) analysis and appropriate statistical analyses.MethodsThis study was a post-hoc GIS-based analysis of OHCA patients in the city of Vancouver and surrounding areas, using data collected by the Resuscitation Outcomes Consortium between September 2007 and December 2011. The kernel density techniques and regression tree analysis using binary recursive partitioning were used.ResultsWe examined 1617 cases of OHCA with a mortality rate of 86.5% (95% CI 84.8-88.2). The mean age of OHCA cases was 66.6 years (95% CI 65.7-67.5), and 33.6% (95% CI 31.3-35.9) were female. The proportion with an initial shockable rhythm (VF or pulseless VT) was 22.2% (95% CI 20.2-24.2); 42.3% (95% CI 39.9-44.7) of all cases received bystander CPR, and 49.7% (95% CI 47.3-52.1) were transported to the hospital by paramedics. The rate of survival to hospital discharge with favourable neurological status (FNS) Cerebral Performance Category (CPC) 1 or 2 was 10.4% (8.9-11.9). Distance of transport to the hospital (less than 2.7 km) was a significant predictor of survival with FNS, but income did not predict survival with FNS. Areas with higher proportions of commuters by car demonstrated lower rates of survival with FNS.ConclusionThis is the first GIS-based study to examine OHCA in a single large Canadian centre. Clustering of OHCA consistent with areas of high population density was observed. Distance of transport was a significant predictor of survival with FNS for patients with OHCA. This may have important implications for future emergency medical services deployment and dispatch decision-making, and public policy initiatives.
Hegel’s Subjective Logic as a Logic for (Hegel’s) Philosophy of Mind
Authors: Redding Paul 
Published: October 2016

AbstractIn the 1930s, C. I. Lewis, who was responsible for the revival of modal logic in the era of modern symbolic logic, characterized ‘intensional’ approaches to logic as typical of post-Leibnizian ‘continental philosophy’, in contrast to the ‘extensionalist’ approaches dominant in the British tradition. Indeed Lewis’s own work in this area had been inspired by the logic of his teacher, the American ‘Absolute Idealist’, Josiah Royce. Hegel’s ‘Subjective Logic’ in Book III of hisScience of Logic, can, I suggest, be considered as an intensional modal logic, and this paper explores parallels between it and a later variety of modal logic—tenselogic, as developed by Arthur Prior in the 1950s and 60s. Like Lewis, Prior too had been influenced in this area by a teacher with strong Hegelian leanings—John N. Findlay. Treated as anintensional(with an ‘s’) logic, Hegel’s subjective logic can be used as a framework for addressing issues ofintentionality(with a ‘t’)—the mind’s capacity to be intentionally directed to objects. In this way, I suggest that the structures of his subjective logic can clarify what is at issue in the ‘Psychology’ section of theEncyclopaediaPhilosophy of Subjective Spirit.
Imperfect Models: The Kingston Lunatic Asylum Scandal and the Problem of Postemancipation Imperialism
Authors: Fryar Christienna D. 
Published: October 2016

AbstractThis article examines an imperial scandal concerning the treatment of patients in the lunatic asylum of Kingston, Jamaica, that highlighted the inadequacies of the imperial government. A significant moment in the development of colonial public health policy, this scandal also spoke to broader questions of postemancipation imperial governance. At the heart of the scandal was a debate about whether standards of treatment developed in Britain—symbolized by the image of the ideal asylum and the ideology of moral management—could and should be implemented in colonies. This debate was all the more fraught because the designation of moral management as the official protocol was recent, its implementation incomplete, and its underlying ideas contested. Nevertheless, despite the instability of these ideas, during the scandal and its aftermath, actors treated them as a monolithic package of standards before making them the definitive model for all colonial institutions. Indeed, the scandal helped further bolster moral management in Britain.