bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastrin induces parathyroid hormone-like hormone expression in gastric parietal cells
Authors: Al Menhali Asma , Keeley Theresa M. , Demitrack Elise S. , Samuelson Linda C. 
Published: April 2017

Parietal cells play a fundamental role in stomach maintenance, not only by creating a pathogen-free environment through the production of gastric acid, but also by secreting growth factors important for homeostasis of the gastric epithelium. The gastrointestinal hormone gastrin is known to be a central regulator of both parietal cell function and gastric epithelial cell proliferation and differentiation. Our previous gene expression profiling studies of mouse stomach identified parathyroid hormone-like hormone (PTHLH) as a potential gastrin-regulated gastric growth factor. Although PTHLH is commonly overexpressed in gastric tumors, its normal expression, function, and regulation in the stomach are poorly understood. In this study we used pharmacologic and genetic mouse models as well as human gastric cancer cell lines to determine the cellular localization and regulation of this growth factor by the hormone gastrin. Analysis of PthlhLacZ/+ knock-in reporter mice localized Pthlh expression to parietal cells in the gastric corpus. Regulation by gastrin was demonstrated by increased Pthlh mRNA abundance after acute gastrin treatment in wild-type mice and reduced expression in gastrin-deficient mice. PTHLH transcripts were also observed in normal human stomach as well as in human gastric cancer cell lines. Gastrin treatment of AGS-E gastric cancer cells induced a rapid and robust increase in numerous PTHLH mRNA isoforms. This induction was largely due to increased transcriptional initiation, although analysis of mRNA half-life showed that gastrin treatment also extended the half-life of PTHLH mRNA, suggesting that gastrin regulates expression by both transcriptional and posttranscriptional mechanisms. NEW & NOTEWORTHY We show that the growth factor parathyroid hormone-like hormone (PTHLH) is expressed in acid-secreting parietal cells of the mouse stomach. We define the specific PTHLH mRNA isoforms expressed in human stomach and in human gastric cancer cell lines and show that gastrin induces PTHLH expression via transcription activation and mRNA stabilization. Our findings suggest that PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis.
Interaction of compass sensing and object-motion detection in the locust central complex
Authors: Bockhorst Tobias , Homberg Uwe 
Published: April 2017

Goal-directed behavior is often complicated by unpredictable events, such as the appearance of a predator during directed locomotion. This situation requires adaptive responses like evasive maneuvers followed by subsequent reorientation and course correction. Here we study the possible neural underpinnings of such a situation in an insect, the desert locust. As in other insects, its sense of spatial orientation strongly relies on the central complex, a group of midline brain neuropils. The central complex houses sky compass cells that signal the polarization plane of skylight and thus indicate the animal’s steering direction relative to the sun. Most of these cells additionally respond to small moving objects that drive fast sensory-motor circuits for escape. Here we investigate how the presentation of a moving object influences activity of the neurons during compass signaling. Cells responded in one of two ways: in some neurons, responses to the moving object were simply added to the compass response that had adapted during continuous stimulation by stationary polarized light. By contrast, other neurons disadapted, i.e., regained their full compass response to polarized light, when a moving object was presented. We propose that the latter case could help to prepare for reorientation of the animal after escape. A neuronal network based on central-complex architecture can explain both responses by slight changes in the dynamics and amplitudes of adaptation to polarized light in CL columnar input neurons of the system.NEW & NOTEWORTHY Neurons of the central complex in several insects signal compass directions through sensitivity to the sky polarization pattern. In locusts, these neurons also respond to moving objects. We show here that during polarized-light presentation, responses to moving objects override their compass signaling or restore adapted inhibitory as well as excitatory compass responses. A network model is presented to explain the variations of these responses that likely serve to redirect flight or walking following evasive maneuvers.
Predictors of oral cavity bleeding and clinical outcome after dental procedures in patients on vitamin K antagonists
Authors: Biedermann Joseph S. , Rademacher Willem M. H. , Hazendonk Hendrika C. A. M. , van Diermen Denise E. , Leebeek Frank W. G. , Rozema Frederik R. , Kruip Marieke J. H. A. 
Published: April 2017

SummaryPatients on vitamin K antagonists (VKA) often undergo invasive dental procedures. International guidelines consider all dental procedures as low-risk procedures, while bleeding risk may differ between standard low-risk (e. g. extraction 1–3 elements) and extensive high-risk (e.g. extraction of >3 elements) procedures. Therefore current guidelines may need refinement. In this cohort study, we identified predictors of oral cavity bleeding (OCB) and evaluated clinical outcome after low-risk and highrisk dental procedures in patients on VKA. Perioperative management strategy, procedure risk, and 30-day outcomes were assessed for each procedure. We identified 1845 patients undergoing 2004 low-risk and 325 high-risk procedures between 2013 and 2015. OCB occurred after 67/2004 (3.3 %) low-risk and 21/325 (6.5 %) high-risk procedures (p=0.006). In low-risk procedures, VKA continuation with tranexamic acid mouthwash was associated with a lower OCB risk compared to continuation without mouthwash [OR=0.41, 95 %CI 0.23–0.73] or interruption with bridging [OR=0.49, 95 %CI 0.24–1.00], and a similar risk as interruption without bridging [OR=1.44, 95 %CI 0.62–3.64]. In high-risk procedures, VKA continuation was associated with an increased OCB risk compared to interruption [OR=3.08, 95 %CI 1.05–9.04]. Multivariate analyses revealed bridging, antiplatelet therapy, and a supratherapeutic or unobjectified INR before the procedure as strongest predictors of OCB. Non-oral cavity bleeding (NOCB) and thromboembolic event (TE) rates were 2.1 % and 0.2 %. Bridging therapy was associated with a two-fold increased risk of NOCB [OR=1.93, 95 %CI 1.03–3.60], but not with lower TE rates. In conclusion, predictors of OCB were mostly related to perioperative management and differed between low-risk and high-risk procedures. Perioperative management should be differentiated accordingly.
Gly74Ser mutation in protein C causes thrombosis due to a defect in protein S-dependent anticoagulant function
Authors: Chen Changming , Yang Likui , Villoutreix Bruno O. , Wang Xuefeng , Ding Qiulan , Rezaie Alireza R. 
Published: April 2017

SummaryProtein C is a vitamin K–dependent serine protease zymogen in plasma which upon activation by thrombin in complex with thrombomodulin (TM) down-regulates the clotting cascade by a feedback loop inhibition mechanism. Activated protein C (APC) exerts its anticoagulant function through protein S-dependent degradation of factors Va and VIIIa. We recently identified a venous thrombosis patient whose plasma level of protein C antigen is normal, but its anticoagulant activity is only 34% of the normal level. Genetic analysis revealed that the proband and her younger brother carry a novel heterozygous mutation c.346G>A, p.Gly74Ser (G74S) in PROC. Thrombin generation assay indicated that the TM-dependent anticoagulant activity of the proband’s plasma has been significantly impaired. We expressed protein C-G74S in mammalian cells and characterised its properties in established coagulation assays. We demonstrate that the protein C variant can be normally activated by the thrombin-TM complex and the resulting APC mutant also exhibits normal amidolytic and proteolytic activities toward both FVa and FVIIIa. However, it was discovered the protein S-dependent catalytic activity of APC variant toward both procoagulant cofactors has been significantly impaired. Protein S concentration-dependence of FVa degradation revealed that the capacity of APC variant to interact with the cofactor has been markedly impaired. The same results were obtained for inactivation of FVa-Leiden suggesting that the protein S-dependent activity of APC variant toward cleavage of Arg-306 site has been adversely affected. These results provide insight into the mechanism through which G74S substitution in APC causes thrombosis in the proband carrying this mutation.
Multiphasic Regulation of Systemic and Peripheral Organ Metabolic Responses to Cardiac Hypertrophy
Authors: Liew Chong Wee , Xu Shanshan , Wang Xuerong , McCann Maximilian , Whang Kong Hyerim , Carley Andrew C. , Pang Jingbo , Fantuzzi Giamila , O’Donnell J. Michael , Lewandowski E. Douglas 
Published: April 2017

Background—
Reduced fat oxidation in hypertrophied hearts coincides with a shift of carnitine palmitoyl transferase I from muscle to increased liver isoforms. Acutely increased carnitine palmitoyl transferase I in normal rodent hearts has been shown to recapitulate the reduced fat oxidation and elevated atrial natriuretic peptide message of cardiac hypertrophy.

Methods and Results—
Because of the potential for reduced fat oxidation to affect cardiac atrial natriuretic peptide, and thus, induce adipose lipolysis, we studied peripheral and systemic metabolism in male C57BL/6 mice model of transverse aortic constriction in which left ventricular hypertrophy occurred by 2 weeks without functional decline until 16 weeks (ejection fraction, −45.6%; fractional shortening, −22.6%). We report the first evidence for initially improved glucose tolerance and insulin sensitivity in response to 2 weeks transverse aortic constriction versus sham, linked to enhanced insulin signaling in liver and visceral adipose tissue (epididymal white adipose tissue [WAT]), reduced WAT inflammation, elevated adiponectin, mulitilocular subcutaneous adipose tissue (inguinal WAT) with upregulated oxidative/thermogenic gene expression, and downregulated lipolysis and lipogenesis genes in epididymal WAT. By 6 weeks transverse aortic constriction, the metabolic profile reversed with impaired insulin sensitivity and glucose tolerance, reduced insulin signaling in liver, epididymal WAT and heart, and downregulation of oxidative enzymes in brown adipose tissue and oxidative and lipogenic genes in inguinal WAT.

Conclusions—
Changes in insulin signaling, circulating natriuretic peptides and adipokines, and varied expression of adipose genes associated with altered insulin response/glucose handling and thermogenesis occurred prior to any functional decline in transverse aortic constriction hearts. The findings demonstrate multiphasic responses in extracardiac metabolism to pathogenic cardiac stress, with early iWAT browning providing potential metabolic benefits.
Fractional Flow Reserve–Guided Complete Revascularization Improves the Prognosis in Patients With ST-Segment–Elevation Myocardial Infarction and Severe Nonculprit Disease
Authors: Lønborg Jacob , Engstrøm Thomas , Kelbæk Henning , Helqvist Steffen , Kløvgaard Lene , Holmvang Lene , Pedersen Frants , Jørgensen Erik , Saunamäki Kari , Clemmensen Peter , De Backer Ole , Ravkilde Jan , Tilsted Hans-Henrik , Villadsen Anton Boel , Aarøe Jens , Jensen Svend Eggert , Raungaard Bent , Køber Lars , Høfsten Dan Eik 
Published: April 2017

Background—
The impact of disease severity on the outcome after complete revascularization in patients with ST-segment–elevation myocardial infarction and multivessel disease is uncertain. The objective of this post hoc study was to evaluate the impact of number of diseased vessel, lesion location, and severity of the noninfarct-related stenosis on the effect of fractional flow reserve–guided complete revascularization.

Methods and Results—

In the DANAMI-3-PRIMULTI study (Primary PCI in Patients With ST-Elevation Myocardial Infarction and Multivessel Disease: Treatment of Culprit Lesion Only or Complete Revascularization), we randomized 627 ST-segment–elevation myocardial infarction patients to fractional flow reserve–guided complete revascularization or infarct-related percutaneous coronary intervention only. In patients with 3-vessel disease, fractional flow reserve–guided complete revascularization reduced the primary end point (all-cause mortality, reinfarction, and ischemia-driven revascularization; hazard ratio [HR], 0.33; 95% confidence interval [CI], 0.17–0.64;
P
=0.001), with no significant effect in patients with 2-vessel disease (HR, 0.77; 95% CI, 0.47–1.26;
P
=0.29;
P
for interaction =0.046). A similar effect was observed in patients with diameter stenosis ≥90% of noninfarct-related arteries (HR, 0.32; 95% CI, 0.18–0.62;
P
=0.001), but not in patients with less severe lesions (HR, 0.72; 95% CI, 0.44–1.19;
P
=0.21;
P
for interaction =0.06). The effect was most pronounced in patients with 3-vessel disease and noninfarct-related stenoses ≥90%, and in this subgroup, there was a nonsignificant reduction in the end point of mortality and reinfarction (HR, 0.32; 95% CI, 0.08–1.32;
P
=0.09). Proximal versus distal location did not influence the benefit from complete revascularization.

Conclusions—
The benefit from fractional flow reserve–guided complete revascularization in ST-segment–elevation myocardial infarction patients with multivessel disease was dependent on the presence of 3-vessel disease and noninfarct diameter stenosis ≥90% and was particularly pronounced in patients with both of these angiographic characteristics.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT01960933.
Determinants of leadership in groups of female mallards
Authors: Bousquet Christophe A.H. , Ahr Noëlle , Sueur Cédric , Petit Odile 
Published: April 2017

When moving in groups, social animals tend to follow a leader which successfully attracted them. Many variables are known to affect an individual’s propensity to act as a leader. Depending on their nature, these variables underlie two theoretical paradigms (i) ‘leadership according to social indifference’, characterised by differences in personality or sociability, or (ii) ‘leadership according to need’, characterised by differences in energetic requirements or information content. Currently, it is not clear under which circumstances each of the two paradigms plays a larger role. Here, we tried to understand these paradigms by observing collective movements in female mallards. Each of these mallards previously learned individually to associate one of four locations in a maze with food rewards. We then formed groups of various compositions (group size range: 2–5 individuals) with respect to personality, sociability, energetic requirements, motivation and information content. We found that groups remained cohesive, and that certain individuals were consistent leaders within and between trials. The order of entering the maze was mainly determined by energetic requirements. However, soon after entering the maze, the progression order changed. Then, more socially indifferent individuals took the lead and this new order remained constant until all individuals reached the final location, which was usually the one the leader had learned. In addition, we investigated the role of naïve individuals in group decision-making. In our setup, adding naïve individuals broke the leadership consistency between trials and increased fission events. Overall, our results show that the onset of collective movements may be driven by different mechanisms compared to the movement progression itself.
A Physical Interpretation of the Wind-Wave Instability as Interacting Waves
Authors: Carpenter J. R. , Guha A. , Heifetz E. 
Published: April 2017

AbstractOne mechanism for the growth of ocean surface waves by wind is through a shear instability that was first described by Miles in 1957. A physical interpretation of this wind-wave instability is provided in terms of the interaction of the surface gravity wave with perturbations of vorticity within the critical layer—a near-singularity in the airflow where the background flow speed matches that of the surface gravity wave. This physical interpretation relies on the fact that the vertical velocity field is slowly varying across the critical layer, whereas both the displacement and vorticity fields vary rapidly. Realizing this allows for the construction of a physically intuitive description of the critical layer vorticity perturbations that may be approximated by a simple vortex sheet model, the essence of the wind-wave instability can then be captured through the interaction of the critical layer vorticity with the surface gravity wave. This simple model is then extended to account for vorticity perturbations in the airflow profile outside of the critical layer and is found to lead to an exact description of the linear stability problem that is also computationally efficient. The interpretation allows, in general, for the incorporation of sheared critical layers into the “wave interaction theory” that is commonly used to provide a physical description and rationalization of results in the stability of stratified shear flows.
A New Radio Frequency Interference Filter for Weather Radars
Authors: Cho John Y. N. 
Published: April 2017

AbstractA new radio frequency interference (RFI) filter algorithm for weather radars is proposed in the two-dimensional (2D) range-time/sample-time domain. Its operation in 2D space allows RFI detection at lower interference-to-noise or interference-to-signal ratios compared to filters working only in the sample-time domain while maintaining very low false alarm rates. Simulations and real weather radar data with RFI are used to perform algorithm comparisons. Results are consistent with theoretical considerations and show the 2D RFI filter to be a promising addition to the signal processing arsenal against interference with weather radars. Increased computational burden is the only drawback relative to filters currently used by operational systems.
Performance of Basin-Scale HWRF Tropical Cyclone Track Forecasts
Authors: Alaka Ghassan J. , Zhang Xuejin , Gopalakrishnan Sundararaman G. , Goldenberg Stanley B. , Marks Frank D. 
Published: April 2017

Abstract
The Hurricane Weather Research and Forecasting (HWRF) Model is a dynamical model that has shown annual improvements in its tropical cyclone (TC) track forecasts as a result of various modifications. This study focuses on an experimental version of HWRF, called the basin-scale HWRF (HWRF-B), configured with 1) a large, static outer domain to cover multiple TC basins and 2) multiple sets of high-resolution movable nests to produce forecasts for several TCs simultaneously. Although HWRF-B and the operational HWRF produced comparable average track errors for the 2011–14 Atlantic hurricane seasons, strengths of HWRF-B are identified and linked to its configuration differences. HWRF-B track forecasts were generally more accurate compared with the operational HWRF when at least one additional TC was simultaneously active in the Atlantic or east Pacific basins and, in particular, when additional TCs were greater than 3500 km away. In addition, at long lead times, HWRF-B average track errors were lower than for the operational HWRF for TCs initialized north of 25°N or west of 60°W, highlighting the sensitivity of TC track forecasts to the location of the operational HWRF’s outermost domain. A case study, performed on Hurricane Michael, corroborated these HWRF-B strengths. HWRF-B shows the potential to serve as an effective bridge between regional modeling systems and next-generational global efforts.
Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin–biotin immunoassays
Authors: Trambas Christina , Lu Zhong , Yen Tina , Sikaris Ken 
Published: April 2017

Background Biotin interference in streptavidin-based immunoassays causes widespread analytical distortions that may lead to clinical confusion, inappropriate patient management and, ultimately, adverse events. Its prevalence has increased recently due to the increased use of high-dose biotin therapy in specific patient groups (notably multiple sclerosis) and possibly the general community. Methods We have developed a method to deplete biotin from samples using the streptavidin-coated magnetic microparticles that are a component of most susceptible assays. Results We show that high concentrations of spiked biotin can be adequately depleted from serum using this approach, and that gross biochemical derangements can be restored to normality. We also show that biotin in samples derived from multiple sclerosis patients receiving 300 mg biotin daily can be adequately depleted to remove associated analytical interference and restore normal results. The method is applicable to competitive and sandwich immunoassays and importantly, because it does not change the volume of the sample, suitable for the measurement of free thyroid hormone assays. Application of the method does not significantly change the precision of measurement, and for the majority of analytes, the accuracy is not substantially altered. Conclusions Adopting this method enables laboratories to confirm biotin interference in the appropriate clinical setting. Moreover, it enables laboratories to remove the interference and report accurate and reliable results, without the need for patients to withhold beneficial therapy prior to blood tests. Until the biotin tolerance of susceptible assays is improved, our method gives laboratories a safe alternative for reporting results using streptavidin-based methods.
High-dose vitamin D supplementation is associated with an improvement in several cardio-metabolic risk factors in adolescent girls: a nine-week follow-up study
Authors: Khayyatzadeh Sayyed Saeed , Mirmoosavi Seyed Jamal , Fazeli Mostafa , Abasalti Zahra , Avan Amir , Javandoost Ali , Rahmani Farzad , Tayefi Maryam , Hanachi Parichehr , Ferns Gordon A , Bahrami-Taghanaki Hamidreza , Ghayour-Mobarhan Majid 
Published: April 2017

Background Vitamin D deficiency is a prevalent and important global health problem. Because of its role in growth and development, vitamin D status is likely to be particularly important in adolescent girls. Here, we explored the effects of high-dose vitamin D supplementation on cardio-metabolic risk factors. Methods We have examined the effects of vitamin D supplementation on cardio-metabolic risk factors in 988 healthy adolescent girls in Iran. Fasting blood samples and anthropometric measurements were obtained at baseline and after supplementation with high-dose vitamin D. All individuals took a capsule of 50,000 IU vitamin D/week for nine weeks. The study was completed by 940 participants. Results The prevalence of vitamin D deficiency was 90% at baseline, reducing to 16.3% after vitamin D supplementation. Vitamin supplementation was associated with a significant increase in serum concentrations of 25 (OH) vitamin D and calcium. There were significant reductions in diastolic blood pressure, heart rate, waist circumference and serum fasting blood glucose, total- and low-density lipoprotein-cholesterol after the nine-week period on vitamin D treatment, but no significant effects were observed on body mass index, systolic blood pressure or serum high-density lipoprotein cholesterol and triglyceride. Conclusion Vitamin D supplementation had beneficial effects on cardio-metabolic profile in adolescent girls.