bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of legislative basis for medical rehabilitation in Russia
Authors: Voynovskiy A. E. , Efanov A. E. 
Published: September 2024

Background. In recent years, there have been significant changes in the regulatory documentation related to medical rehabilitation, caused by the need to consolidate the principles for rehabilitation of patients in post-COVID period and standardize the procedures used for it. Therefore, it is important to analyze the existing regulatory framework and the proposed changes that will come into force in the near future.Objective: to assess the regulatory legal acts governing the provision of medical rehabilitation in Russia.Material and methods. Regulatory legal acts and their drafts were obtained through the reference and legal systems “Kontur Standard”, “ConsultantPlus”, and the Federal Portal of Draft Regulatory Legal Acts. The following electronic libraries, databases and other sources were used to collect secondary information: eLibrary, Google Scholar, PubMed/MEDLINE, Yandex and social networks.Results. Article 41 of the Constitution of the Russian Federation guarantees the right to health protection and medical care. Within the latter the use of medical rehabilitation can be considered. Going down to the next level, the term “medical rehabilitation” is found in Federal Law dated November 27, 2011 No. 323-FZ “On the fundamentals of health protection of citizens in the Russian Federation”. However, the requirements in the field under consideration are specifically described in Orders of the Ministry of Health of Russia No. 788n and 878n, which establish a three-stage provision of medical rehabilitation and give specific time instructions for the frequency and duration of care prcedures. The conditions for the transition of patients between the stages of medical rehabilitation are presented, and creating interdisciplinary rehabilitation teams is specified.Conclusion. The analysis of innovations in the regulatory framework showed no fundamental changes in the regulation of medical rehabilitation. At the moment, there is a need to expand it by regulating the activities of multidisciplinary rehabilitation teams. A more detailed elaboration of normative documentation regulating the provision of medical rehabilitation to children is necessary.
Estratégias de Ensinagem e Hibridismo – Análise, Desenvolvimento e Aplicação
Authors: Macedo Alcina Maria Barcellos de Melo e , Oliveira Ramony Maria da Silva Reis 
Published: September 2024

Os períodos peri- e pós-pandemia de COVID-19 revelaram fragilidades do sistema educacional brasileiro destacando a necessidade de desenvolvimento de metodologias inovadoras para se adaptar ao “novo normal” e integrar a era digital às práticas escolares. Este trabalho teve por objetivo desenvolver estratégias de ensinagem hibridizadas para potencializar a aprendizagem de alunos do Ensino Médio Integrado. As principais técnicas e ferramentas de coleta e análise de dados utilizadas foram a Pesquisa Bibliográfica, a Entrevista Semiestruturada, as Análises Documental e de Conteúdo. O trabalho incluiu o levantamento teórico e a escolha das estratégias de ensinagem a serem hibridizadas, bem como a construção dos respectivos modelos de hibridização. O projeto piloto ocorreu em turma do Curso Técnico de Química do Instituto Federal de Educação, Ciência e Tecnologia do Norte de Minas Gerais (IFNMG). A escolha da turma foi baseada em critérios estabelecidos pelo professor aplicador que decidiu participar após reunião com a pesquisadora principal. O modelo de hibridização (Tempestade Cerebral) escolhido, adaptado e utilizado foi definido pelo professor aplicador a partir das necessidades docentes, das especificidades da turma e dos recursos didáticos disponíveis. A aplicação ocorreu sem percalços, sendo o direcionamento do professor decisivo para isto. A partir dos relatos obtidos na entrevista, evidencia-se a preponderância do papel do professor na compreensão das possibilidades que podem ser vislumbradas com a implementação de estratégias de ensinagem em uma metodologia híbrida. Destaca-se o comprometimento e a decisiva atuação do professor aplicador no sentido de motivar os alunos a participarem de forma ativa e corresponsável.

Palavras-chave: Educação profissional. Metodologia de ensino. Aprendizagem híbrida.
A AVALIAÇÃO PSICOLÓGICA NO ESTÁGIO BÁSICO EM PROCESSOS CLÍNICOS E AVALIAÇÃO PSICOLÓGICA: PELO OLHAR DOS BOLSISTAS
Authors: Muller Cristiane , Fleck Giovana Pereira , Mentz de Souza Pedro Henrique , Gonzatti Valéria 
Published: September 2024

A disciplina é fundamental na formação em psicologia, proporcionando uma base teórica, técnica e de conhecimentos necessários para conduzir avaliações psicológicas (AP). O objetivo é relatar as experiências, de bolsistas, das etapas de preparação e realização dos atendimentos de AP. A primeira fase da disciplina compreende a revisão de conteúdos que embasam a prática de AP. São realizados momentos de simulação de situações hipotéticas de casos para o exercício de como proceder diante de cada situação. Próximo ao início dos atendimentos, os estudantes recebem os casos, se organizam quanto ao papel que terão durante os atendimentos e definem um plano de atendimento. A etapa de preparação é importante tanto para o êxito do processo de avaliação quanto para garantir maior segurança e conhecimento aos estudantes, de forma que observa-se que os estudantes preocupam-se e realizam movimentos de reflexões e envolvimento quanto ao seu aprimoramento. No entanto, também existem problemáticas envolvendo as dificuldades na preparação em relação à disciplina bimestral, como o tempo bem mais limitado, a preparação muito mais intensa e os alunos que trabalham durante o dia acabam sendo prejudicados, pois não conseguem realizar as revisões de forma aprofundada. Por isso, considera-se muito importante que a disciplina ocorra de forma semestral, garantindo uma melhor preparação. A preparação para AP demonstrou ser essencial para o desenvolvimento dos estagiários. A combinação de revisões oferece uma base sólida para enfrentar desafios clínicos reais no campo da AP. Através do aprofundamento teórico e prático, os bolsistas enriquecem seu próprio repertório de habilidades e servem como incentivo aos estagiários. Como resultado, a disciplina não apenas cria profissionais competentes, mas também influencia positivamente a qualidade geral da educação e profissão.
Rancang Bangun Sistem Informasi Kelola Magang (SIKAM) di LPTP Surakarta
Authors: Yudhanto Yudho , Albab Hypatia Ulil 
Published: September 2024

<p><strong>Abstrak </strong></p><p>LPTP telah memiliki website yang berfungsi sebagai wadah informasi yang berguna untuk menyampaikan informasi terkait LPTP kepada masyarakat. Saat ini, proses update artikel yang dibuat dari luar masih lama dikarenakan harus melalui email kepada admin. Permasalahan berikutnya adalah tentang proses pengajuan magang di LPTP yang masih manual manual dan harus offline dikombinasikan menggunakan email. Sehingga menimbulkan permasalahan yang ketiga yakni sulitnya memantau kinerja peserta magang yang belum tersimpan didalam database, akibatnya banyak data peserta magang yang tidak terarsip. Solusi yang diusulkan adalah dengan membuat sebuah sistem informasi yang digunakan untuk mengelola data artikel dan data peserta magang. Aktor yang terlibat adalah admin, pegawai dan masyarakat. Perancangan dan Pembuatan Sistem Informasi Kelola Magang (SIKAM) dibuat dengan perancangan analisis permodelan Bisnis Proses, Kebutuhan Perangkat Lunak, Use Case Diagram, Activity Diagram. Dengan metode Prototyping maka digunakanlah bahasa pemrograman PHP dengan menggunakan framework CodeIgniter 3, dan perancangan basis data dengan menggunakan permodelan Entity Relationship Diagram. Untuk penyimpanan menggunakan basis data MySQL. Kesimpulan dari pengujian menunjukkan bahwa dari 34 skenario yang dijalankan dengan metode Black box didapatkan hasil berjalan dengan baik dan memuaskan.</p><p>================</p><p><strong><em>Abstract</em></strong></p><p><em><em>LPTP already has a website that functions as an information forum that is useful for conveying information related to LPTP to the public. Currently, the process of updating articles made from outside still takes a long time because they have to be emailed to the admin. The next problem is the process of applying for internships at LPTP which is still manual and must be offline combined using email. So that raises the third problem, namely the difficulty of monitoring the performance of apprentices who have not been stored in the database, as a result, a lot of apprentice data is not archived. The proposed solution is to create an information system that is used to manage article data and apprentice data. The actors involved are admin, employees and the community. The design and manufacture of Intern Management Information Systems (SIKAM) is made by designing business process modeling analysis, Software Requirements, Use Case Diagrams, Activity Diagrams. With the Prototyping method, the PHP programming language is used using the CodeIgniter 3 framework, and database design using the Entity Relationship Diagram model. For storage using MySQL database. The conclusion of the test shows that of the 34 scenarios that were run using the Black-box method, the results went well and were satisfactory.</em></em><em></em></p>
Fragment based discovery of a small molecule RhoGDI2 ligand HR3119 that inhibits cancer cell metastasis
Authors: Ruan Ke , Liu Mingqing , Wan Shizhang , Guo Shangxi , Liu Jiuyang , Li Wenqian , Wang Lei , Li Fudong , Hai Zhang , Liu Xing , Liu Dan , Gao Jia , He Wei , Yao Xuebiao 
Published: September 2024

Abstract

Guanine nucleotide dissociation inhibitors (GDIs) proteins including RhoGDI2 play a central role in regulating the functions of Ras superfamily proteins that are known to be important cancer drug targets. Given the known challenges in directly targeting Ras superfamily proteins with small compounds, targeting GDIs represents a unique opportunity but has met with limited success. Herein, we describe the discovery of the first small compound HR3119 that binds RhoGDI2 in low micromolar affinity (Kd ~ 7 μM) starting from a millimolar binding affinity fragment hit (Kd ~ 0.7 mM) through iterative structure-guided design. We identify that HR3119 surrogates occupy the binding interface between RhoGDI2 and its endogenous ligand Rac1 in a series of co-crystal structures, laying out rich information for future compound design. We further demonstrate that HR3119 disrupts RhoGDI2-Rac1 binding and promotes the degradation of Rac1, as well as engages RhoGDI2 in cells. As an illustration of this novel chemical probe, we show that HR3119 suppressed the invasion and migration of invasive breast cancer cells at a 5 μM concentration and in a concentration-dependent manner. Interestingly, (6R)-HR3119 exhibited a 100-fold binding affinity advantage to that of (6S)-HR3119 (4 μM vs 290 μM) and improved anti-metastatic activity compared to racemic HR3119. This work provides valuable insights into the discovery of small compounds targeting GDIs in terms of methodology, chemistry starting points, compound design, and phenotype studies, underscoring exciting new perspectives in early drug discovery.
Evaluation of Positioning Accuracy and Clinical Outcomes in 3D Printing- Assisted Ankle Ligament Surgery
Authors: Luo Kai , Xiang Yi , Lu Kang , Du zhongbao , Ren Yanmei , Sun Liqin , Zhang Jian , Qiao Wenjie , Tang Xiangyu 
Published: September 2024

Abstract

Background: To investigate the positioning accuracy and clinical efficacy of 3D-printed, personalized guide plate assisted lateral ankle ligament reconstruction.
Methods: We retrospectively studied 48 patients with chronic lateral ankle instability and divided them into two groups: the template group (24 patients) and the conventional group (24 patients). The operation time, intraoperative X-ray exposure time, American Orthopedic Foot and Ankle Society and Foot and Ankle Outcome Score (FAOS) were compared between the two groups.
Results: All patients were followed up for 12-20 months (14.5 ± 5.5). The operation time in the template group was 32-55 min (38.6 ± 9.7), the operation time in the conventional group was 48-73 min (58.3 ± 12.6), and there was a significant difference between the two groups (P<0.05). The number of X-ray exposures in the template group was 1-3 (1.2 ± 0.6), the number of X-ray exposures in the conventional group was 3-6 (3.8 ± 2.2), and there was a significant difference between the two groups (p<0.05). There was no significant difference in the AOFAS score or FAOS score between the two groups before the operation orat the last follow-up after the operation. There was no significant difference in the talar anteversion distance or talar varus angle between the two groups at the last follow-up after the operation.
Conclusion: Comparedwith conventional surgery, 3D-printedtemplate surgery has a shorter operation time, higher positioning accuracy, and shorterradiation exposure time. 3D printing template surgery is a successful treatment option with the same satisfactory effect on patients with chronic lateral ankle instability.
Expression of anti-amyloid CARs in microglia promotes efficient and selective phagocytosis of Aβ1‒42
Authors: Heiss Christina , Riise Rebecca , Hanse Eric , Fruhwürth Stephanie , Zetterberg Henrik , Björefeldt Andreas 
Published: September 2024

Abstract

Genetic engineering of microglial cells is a promising therapeutic avenue emerging with advancements in gene delivery techniques. Using a recently developed AAV capsid for efficient in vitro transduction we report the engineering of microglia with CARs (CAR-Mic) targeting phagocytosis of amyloid beta 1‒42 (Aβ42). Functional screening of seven CAR constructs in human iPSC-derived microglia revealed up to 6-fold increases in internalized Aβ relative to viral control. CAR-driven phagocytic enhancement was selective for Aβ, dependent on intracellular domain signaling, and was confirmed in primary mouse microglia. These findings highlight the potential of using this approach to target dysfunctional microglia in Alzheimer’s disease and other CNS disorders.
Reversal Gene Expression Assessment for Drug Repurposing, a Case Study of Glioblastoma
Authors: Sun Shixue , Shyr Zeenat , McDaniel Kathleen , Fang Yuhong , Tao Dingyin , Chen Catherine Z. , Zheng Wei , Zhu Qian 
Published: September 2024

Abstract

Glioblastoma (GBM) is a rare brain cancer with an exceptionally high mortality rate, which illustrates the pressing demand for more effective therapeutic options. Despite considerable research efforts on GBM, its underlying biological mechanisms remain unclear. Furthermore, none of the United States Food and Drug Administration (FDA) approved drugs used for GBM deliver satisfactory survival improvement. This study presents a novel computational pipeline by utilizing gene expression data analysis for GBM for drug repurposing to address the challenges in rare disease drug development, particularly focusing on GBM. The GBM Gene Expression Profile (GGEP) was constructed with multi-omics data to identify drugs with reversal gene expression to GGEP from the Integrated Network-Based Cellular Signatures (iLINCS) database. We prioritized the candidates via hierarchical clustering of their expression signatures and quantification of their reversal strength by calculating two self-defined indices based on the GGEP genes’ log2 foldchange (LFCs) that the drug candidates could induce. Among eight prioritized candidates, in-vitro experiments validated Clofarabine and Ciclopirox as highly efficacious in selectively targeting GBM cancer cells. The success of this study illustrated a promising avenue for accelerating drug development by uncovering underlying gene expression effect between drugs and diseases, which can be extended to other rare diseases and non-rare diseases.
Novel small fragment removal system may improve extraction of renal calculi: an in vitro study.
Authors: Lazarus John , Wellmann Mark , Wulfsberg Jørgen , Ceccato Tommaso , John Jeff 
Published: September 2024

Abstract

Objective
To describe a novel Small Fragment Removal System (SFRS) which is hypothesized to improve stone fragment removal during flexible ureteroscopy in patients with urolithiasis.
The SFRS consists of three parts: a Syphon Ureteric Access Sheath (SUAS), a Dual Action Pump (DAP) and an Agitator.
This bench assessment aims to assess the SFRS’s impact on intra-renal pressure (IRP), irrigant flow rate and stone fragment removal compared to a traditional UAS.
Materials and Methods
A validated phantom kidney and fibre optic pressure sensor was used to assess IRP. Standardized irrigation via a flexible ureterorenoscope was instilled through a traditional UAS and compared to the novel SFRS. Both were 11/13Fr in size. Measured minute volume, IRP and percentage of stone fragments removed were compared.
Results
The mean IRP using a traditional UAS and SFRS was 24,3mmHg and 9,4mmHg respectively. The flow rate of the traditional UAS was 25mL/min, compared to 31mL/min with the SFRS attached.
During bolus fluid administration using the traditional UAS the maximum IRP increased to 41mmHg, compared to 9,3mmHg with the SFRS attached.
A mean of 42,7% (0,103g of 0,305g) of weighed dry stones were removed with the traditional UAS compared to 77,6% (0,233g of 0,299g) with the SFRS attached, p=0,017.
Conclusions
The described Small Fragment Removal System (SFRS) is different from traditional UASs by incorporating a syphoning mechanism. In addition, it has a Dual Action Pump which both boluses and augments the aspiration of irrigant by the Syphon. It further includes a deflectable Agitator to flush out stone fragments.
Alterations in Nailfold Videocapillaroscopy Among Patients with Connective Tissue Diseases Combined with Pulmonary Arterial Hypertension
Authors: Tang Zhicheng , Yang Fan , Wu Haolin , Zhao Ying , Shen Jingyi , Hong Huiming , Yin Fanzhang , Ma Xiaolei , Geng Linyu , Xu Xue , Wei Yu , Zhang Huayong 
Published: September 2024

Abstract

Objectives: This study investigates the correlation between abnormalities detected in nailfold videocapillaroscopy and pulmonary hypertension in patients with connective tissue disease (CTD). Additionally, it explores the potential diagnostic and predictive value of nailfold videocapillaroscopy in assessing microcirculation alterations in these patients.
Methods: A cross-sectional study involving 351 CTD patients and 30 healthy controls underwent nailfold videocapillaroscopy. Two independent physicians assessed the results qualitatively, semi-quantitatively, and quantitatively. Clinical characteristics and laboratory parameters were also recorded, with a focus on differences between CTD patients with pulmonary arterial hypertension (CTD-PAH) and those without (CTD-non-PAH).
Results: Among the patients, 16.5% (n=58) had pulmonary hypertension. CTD-PAH patients showed higher nailfold videocapillaroscopy scores (5.73±3.54 vs. 4.30±2.98, P=0.001) and larger capillary diameters (17.06±8.22 vs. 14.41±9.25, P=0.044) compared to CTD-non-PAH patients. Factors significantly influencing the nailfold videocapillaroscopy score included Raynaud's phenomenon, pulmonary hypertension, and the presence of anti-Scl-70 antibody. The ROC analysis yielded an AUC of 0.621 for predicting PAH. Additionally, pulmonary artery systolic pressure in CTD-PAH patients was positively correlated with both nailfold videocapillaroscopy score (R=0.618, B=3.26, P<0.001) and capillary diameter (R=0.541, B=1.23, P<0.001).
Conclusion: Nailfold videocapillaroscopy is a valuable tool in assessing CTD patients with pulmonary hypertension, providing a novel non-invasive method for evaluating this condition.
Cellular indexing of transcriptomes and epitopes (CITE-Seq) in hidradenitis suppurativa identifies dysregulated cell types in peripheral blood and facilitates diagnosis via machine learning
Authors: Liao Wilson , Kumar Sugandh , Orcales Faye , Shih Bobby , Fang Xiaohui , Yin Congcong , Yates Ashley , Dimitrion Peter , Neuhaus Isaac , Johnson Chandler , Adrianto Indra , Wiala Antonia , Hamzavi Iltefat H. , Zhou Li , Naik Haley , Posch Christian , Mi Qing-Sheng 
Published: September 2024

Abstract

Hidradenitis suppurativa (HS) is a chronic inflammatory skin condition characterized by painful nodules, abscesses, and scarring, predominantly affecting intertriginous regions. This study aimed to utilize single cell RNA and cell-surface protein sequencing (CITE-Seq) to delineate the immune composition of circulating cells in Hidradenitis suppurativa (HS) peripheral blood compared to healthy controls. CITE-Seq was used to analyze the gene and protein expression profiles of peripheral blood mononuclear cells (PBMCs) from 9 HS and 29 healthy controls. The study identified significant differences cell composition between HS patients and healthy controls, including increased proportions of CD14+ and CD16+ monocytes, cDC2, plasmablasts, and proliferating CD4+ T cells in HS patients. Differential expression analysis revealed upregulation of inflammatory markers such as TNF, IL1B, and NF-κB in monocytes, as well as chemokines and cell adhesion molecules involved in immune cell recruitment and tissue infiltration. Pathway enrichment analysis highlighted the involvement of IL-17, IL-26 and TNF signaling pathways in HS pathogenesis. Machine learning identified key markers for diagnostics and therapeutic development. The findings also support the potential for machine learning models to aid in the diagnosis of HS based on immune cell markers. These insights may inform future therapeutic strategies targeting specific immune pathways in HS.
Prognostic Impact of Renal Microcirculatory Dysfunction in Heart Failure Assessed by the Advanced Doppler technique, Superb Microvascular Imaging
Authors: Kayama Kiyomi , Kikuchi Shohei , Sugimoto Tadafumi , Seo Yoshihiro 
Published: September 2024

Abstract

The critical role of cardio-renal interactions in heart failure (HF) prognosis has gained increasing recognition, yet standardized methods for their assessment remain elusive. This study introduces a novel approach utilizing Superb Microvascular Imaging (SMI), an advanced ultrasound technique enabling detailed microvascular flow visualization, to evaluate renal microcirculation. We conducted a retrospective analysis of 78 patients who underwent renal ultrasonography with SMI between October 2020 and May 2023. Temporal changes in the Vascular Index (VI), which quantifies the blood flow signal area within the region of interest on SMI images, were measured. Key parameters included Maximum VI (Max.VI), Minimum VI (Min.VI), and the cyclic variation of VI, calculated as the intrarenal perfusion index (IRPI) = (Max.VI - Min.VI) / Max.VI within one cardiac cycle. The primary endpoint was a composite event (CE), defined as all-cause mortality or unplanned hospitalization due to worsening HF. Over a mean follow-up period of 1.6 ± 0.8 years, 13 of 78 patients (17%) experienced CEs. Patients with CEs exhibited significantly lower Max.VI and Min.VI values, while IRPI was significantly elevated in this group compared to those without CEs. Univariable Cox regression analyses revealed significant associations between Max.VI, Min.VI, and IRPI with CEs. In multivariable Cox regression analyses, Max.VI and Min.VI maintained significant associations with CEs after adjusting for creatinine, estimated central venous pressure, and intra-renal venous flow pattern. Kaplan-Meier analysis demonstrated that Max.VI (< 0.31, as determined by ROC analysis; 43% vs. 7%, log-rank p < 0.001), Min.VI (< 0.08, 42% vs. 8%, log-rank p < 0.001), and IRPI (> 0.70, 39% vs. 10%, log-rank p = 0.002) could effectively stratify CE prognosis. This novel application of SMI for renal circulation assessment provides valuable insights into HF prognosis and enables risk stratification beyond conventional markers.