bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Single Residue Mutation in the Gαq Subunit of the G Protein Complex Causes Blindness in Drosophila
Authors: Cao Jinguo , Bollepalli Murali K , Hu Yuhui , Zhang Jin , Li Qiang , Li Hongmei , Chang Hua , Xiao Feng , Hardie Roger C , Rong Yikang S , Hu Wen 
Published: November 2017

Abstract
Heterotrimeric G proteins play central roles in many signaling pathways, including the phototransduction cascade in animals. However, the degree of involvement of the G protein subunit Gαq is not clear since animals with previously reported strong loss-of-function mutations remain responsive to light stimuli. We recovered a new allele of Gαq in Drosophila that abolishes light response in a conventional electroretinogram assay, and reduces sensitivity in whole-cell recordings of dissociated cells by at least five orders of magnitude. In addition, mutant eyes demonstrate a rapid rate of degeneration in the presence of light. Our new allele is likely the strongest hypomorph described to date. Interestingly, the mutant protein is produced in the eyes but carries a single amino acid change of a conserved hydrophobic residue that has been assigned to the interface of interaction between Gαq and its downstream effector, PLC. Our study has thus uncovered possibly the first point mutation that specifically affects this interaction in vivo.
Identification of Isthmin 1 as a Novel Clefting and Craniofacial Patterning Gene in Humans
Authors: Lansdon Lisa A , Darbro Benjamin W , Petrin Aline L , Hulstrand Alissa M , Standley Jennifer M , Brouillette Rachel B , Long Abby , Mansilla M Adela , Cornell Robert A , Murray Jeffrey C , Houston Douglas W , Manak J Robert 
Published: November 2017

Abstract
Orofacial clefts are one of the most common birth defects, affecting 1–2 per 1000 births, and have a complex etiology. High-resolution array-based comparative genomic hybridization has increased the ability to detect copy number variants (CNVs) that can be causative for complex diseases such as cleft lip and/or palate. Utilizing this technique on 97 nonsyndromic cleft lip and palate cases and 43 cases with cleft palate only, we identified a heterozygous deletion of Isthmin 1 in one affected case, as well as a deletion in a second case that removes putative 3′ regulatory information. Isthmin 1 is a strong candidate for clefting, as it is expressed in orofacial structures derived from the first branchial arch and is also in the same “synexpression group” as fibroblast growth factor 8 and sprouty RTK signaling antagonist 1a and 2, all of which have been associated with clefting. CNVs affecting Isthmin 1 are exceedingly rare in control populations, and Isthmin 1 scores as a likely haploinsufficiency locus. Confirming its role in craniofacial development, knockdown or clustered randomly interspaced short palindromic repeats/Cas9-generated mutation of isthmin 1 in Xenopus laevis resulted in mild to severe craniofacial dysmorphologies, with several individuals presenting with median clefts. Moreover, knockdown of isthmin 1 produced decreased expression of LIM homeobox 8, itself a gene associated with clefting, in regions of the face that pattern the maxilla. Our study demonstrates a successful pipeline from CNV identification of a candidate gene to functional validation in a vertebrate model system, and reveals Isthmin 1 as both a new human clefting locus as well as a key craniofacial patterning gene.
Sgs1 Binding to Rad51 Stimulates Homology-Directed DNA Repair in Saccharomyces cerevisiae
Authors: Campos-Doerfler Lillian , Syed Salahuddin , Schmidt Kristina H 
Published: November 2017

Abstract
Accurate repair of DNA breaks is essential to maintain genome integrity and cellular fitness. Sgs1, the sole member of the RecQ family of DNA helicases in Saccharomyces cerevisiae, is important for both early and late stages of homology-dependent repair. Its large number of physical and genetic interactions with DNA recombination, repair, and replication factors has established Sgs1 as a key player in the maintenance of genome integrity. To determine the significance of Sgs1 binding to the strand-exchange factor Rad51, we have identified a single amino acid change at the C-terminal of the helicase core of Sgs1 that disrupts Rad51 binding. In contrast to an SGS1 deletion or a helicase-defective sgs1 allele, this new separation-of-function allele, sgs1-FD, does not cause DNA damage hypersensitivity or genome instability, but exhibits negative and positive genetic interactions with sae2Δ, mre11Δ, exo1Δ, srs2Δ, rrm3Δ, and pol32Δ that are distinct from those of known sgs1 mutants. Our findings suggest that the Sgs1-Rad51 interaction stimulates homologous recombination (HR). However, unlike sgs1 mutations, which impair the resection of DNA double-strand ends, negative genetic interactions of the sgs1-FD allele are not suppressed by YKU70 deletion. We propose that the Sgs1-Rad51 interaction stimulates HR by facilitating the formation of the presynaptic Rad51 filament, possibly by Sgs1 competing with single-stranded DNA for replication protein A binding during resection.
Clinicopathological study and management of diabetic foot
Authors: L. Chethan , K. M. Amith 
Published: November 2017

Background: The main objectives were to study the bacteriology, pathophysiology of diabetic foot, various limb salvage procedures and outcome, different treatment modalities and newer techniques wherever applicable to prevent complications and to minimise the progression of occurred complication.Methods: 50 patients admitted with diabetic foot presenting with lesions of foot were selected for the study and were managed conservatively and surgical interventions if needed.Results: Majority of the patients in the study revealed history of trauma preceding the foot complications and the chances of injury increased in these patients because of increased incidence of ischemia, neuropathy and infection. 15 patients in this study had neuropathy, 8 patients had ischemia and all cases were culture positive. 16 cases were managed by daily dressing and wound debridement, and slough excision. 9 patients were treated with Split skin graft, 6 patients with incision and drainage (IandD) for abscess and fasciotomy, 10 cases with disarticulation, below knee amputation (BKA) was done in 4 cases and above knee amputation (AKA) in 5 cases.Conclusions: Males are almost four times more affected than females, duration of diabetes varied from 1 year to 22 years and many patients were diagnosed post admission, trivial trauma of some kind was the initiating factor in nearly half of the cases, commonest presenting lesion was Ulcer 52%, conservative treatment consisting of control of diabetes was effective. Wound debridement, slough excision followed by dressing resulted in healing in some cases. Split skin grafts, Disarticulation, below knee amputation and above knee amputations were the other modes treatment.
Impact of the 2010 US Healthy, Hunger-Free Kids Act on School Breakfast and Lunch Participation Rates Between 2008 and 2015
Authors: Vaudrin Nicole , Lloyd Kristen , Yedidia Michael J. , Todd Michael , Ohri-Vachaspati Punam 
Published: November 2017

Objectives. To evaluate National School Lunch Program (NSLP) and School Breakfast Program (SBP) participation over a 7-year period before and after the implementation of the 2010 Healthy, Hunger-Free Kids Act (HHFKA), which required healthier school lunch options beginning in school year (SY) 2012–2013 and healthier school breakfast options beginning in SY2013–2014. Methods. Data were gathered from low-income, high-minority public schools in 4 New Jersey cities. We conducted longitudinal analyses of annual average daily participation (ADP) in school meals among enrolled students overall and among those eligible for free or reduced-price meals. We used linear mixed models to compare NSLP and SBP participation rates from SY2008–2009 to SY2014–2015. Results. NSLP participation rates among students overall differed little across years (from 70% to 72%). SBP rates among enrolled students were stable from the beginning of the study period to SY2013–2014 and then increased from 52% to 59%. Among students eligible for free or reduced-price meals, the ADP was lowest in SY2012–2013 (when the HHFKA was implemented) before rebounding. Conclusions. The HHFKA did not have a negative impact on school meal participation over time. Public Health Implications. The HHFKA-strengthened nutrition standards have not affected school meal participation rates. With time, students are likely to accept healthier options.
Characteristics of HIV-Positive Transgender Men Receiving Medical Care: United States, 2009–2014
Authors: Lemons Ansley , Beer Linda , Finlayson Teresa , McCree Donna Hubbard , Lentine Daniel , Shouse R. Luke ,   for the Medical Monitoring Project
Published: November 2017

Objectives. To present the first national estimate of the sociodemographic, clinical, and behavioral characteristics of HIV-positive transgender men receiving medical care in the United States. Methods. This analysis included pooled interview and medical record data from the 2009 to 2014 cycles of the Medical Monitoring Project, which used a 3-stage, probability-proportional-to-size sampling methodology. Results. Transgender men accounted for 0.16% of all adults and 11% of all transgender adults receiving HIV medical care in the United States from 2009 to 2014. Of these HIV-positive transgender men receiving medical care, approximately 47% lived in poverty, 69% had at least 1 unmet ancillary service need, 23% met criteria for depression, 69% were virally suppressed at their last test, and 60% had sustained viral suppression over the previous 12 months. Conclusions. Although they constitute a small proportion of all HIV-positive patients, more than 1 in 10 transgender HIV-positive patients were transgender men. Many experienced socioeconomic challenges, unmet needs for ancillary services, and suboptimal health outcomes. Attention to the challenges facing HIV-positive transgender men may be necessary to achieve the National HIV/AIDS Strategy goals of decreasing disparities and improving health outcomes among transgender persons.
Use of historical data in flood frequency analysis: a case study for four catchments in Norway
Authors: Engeland Kolbjørn , Wilson Donna , Borsányi Péter , Roald Lars , Holmqvist Erik 
Published: November 2017

Abstract
There is a need to estimate design floods for areal planning and the design of important infrastructure. A major challenge is the mismatch between the length of the flood records and needed return periods. A majority of flood time series are shorter than 50 years, and the required return periods might be 200, 500, or 1,000 years. Consequently, the estimation uncertainty is large. In this paper, we investigated how the use of historical information might improve design flood estimation. We used annual maximum data from four selected Norwegian catchments, and historical flood information to provide an indication of water levels for the largest floods in the last two to three hundred years. We assessed the added value of using historical information and demonstrated that both reliability and stability improves, especially for short record lengths and long return periods. In this study, we used information on water levels, which showed the stability of river profiles to be a major challenge.
The ion-exchanger–loop-stripping process: ammonium recovery from sludge liquor using NaCl-treated clinoptilolite and simultaneous air stripping
Authors: Ellersdorfer Markus 
Published: November 2017

Abstract
The technical feasibility of an ammonium recovery process (‘ion-exchanger–loop-stripping’) for sludge liquor from municipal wastewater treatment plants is examined. The proposed process combines ion-exchange on natural zeolites with simultaneous air stripping of ammonia to produce an industrial NOx-removal agent. Column experiments with continuously recycled NH4Cl-solutions and a real sludge liquor sample were conducted to determine basic ion-exchange kinetics of the applied clinoptilolite. Mass balances of consecutive loading/regeneration cycles show the positive influence of NaCl-pretreatment as well as simultaneous air stripping on the NH4+-exchange capacities. Removal rates for NH4+ between 61.5 and 84.6% were achieved at NH4+-concentrations typical for sludge liquor (900 to 2,300 mg L−1). Zeolite loadings ranged from 5 to 8 mg NH4+ g−1 after 90 min of loading. Regeneration rates were between 42.9 and 49.7%, but increased to 64.8% with simultaneous air stripping. A minimal decrease in the ammonium removal rate was observed as a result of matrix effects in sludge liquor (e.g. flocculants, competing ions). Liquid analyses showed a considerable phosphate-reduction in the sludge liquor sample after ion-exchange due to potential struvite or apatite precipitation. The obtained results enable a detailed design, scale-up and further optimization of the ion-exchanger–loop-stripping process in future.
A Technical Guide to Using Amazon's Mechanical Turk in Behavioral Accounting Research
Authors: Buchheit Steve , Doxey Marcus M. , Pollard Troy , Stinson Shane R. 
Published: November 2017

ABSTRACT
Multiple social science researchers claim that online data collection, mainly via Amazon's Mechanical Turk (MTurk), has revolutionized the behavioral sciences (Gureckis et al. 2016; Litman, Robinson, and Abberbock 2017). While MTurk-based research has grown exponentially in recent years (Chandler and Shapiro 2016), reasonable concerns have been raised about online research participants' ability to proxy for traditional research participants (Chandler, Mueller, and Paolacci 2014). This paper reviews recent MTurk research and provides further guidance for recruiting samples of MTurk participants from populations of interest to behavioral accounting researchers. First, we provide guidance on the logistics of using MTurk and discuss the potential benefits offered by TurkPrime, a third-party service provider. Second, we discuss ways to overcome challenges related to targeted participant recruiting in an online environment. Finally, we offer suggestions for disclosures that authors may provide about their efforts to attract participants and analyze responses.
Information Sharing during Auditors' Fraud Brainstorming: Effects of Psychological Safety and Auditor Knowledge
Authors: Gissel Jodi L. , Johnstone Karla M. 
Published: November 2017

SUMMARY

Conducting a fraud brainstorming session during planning assists with risk-based tailoring of the audit. An effective session should include a team environment in which all members are willing to share information to appropriately calibrate the collective assessment of fraud risk. We report the results of a study (Gissel and Johnstone 2017) in which we manipulate partner leadership in terms of engendering a safe (unsafe) psychological environment whereby subordinates are (are not) encouraged to speak up about fraud-relevant information. Participants are audit staff and seniors, and through the experimental case (based on the Krispy Kreme Doughnut Corporation fraud) they come to realize that they alone possess fraud-relevant information critical to the team's calibration of fraud risk. These auditors participate in a simulated brainstorming session containing the partner leadership manipulation and indicate changes in their willingness to share the fraud-relevant information with the team. We find that less-knowledgeable auditors become more willing to share their privately known, fraud-relevant information when the partner engenders a safe psychological environment as compared to an unsafe environment. In contrast, more-knowledgeable auditors are impervious to the relative psychological safety engendered by the partner; these individuals are equally willing to speak up regardless of the partner's leadership behavior.
Data Availability: Contact the authors.
Standardizing Clinically Meaningful Outcome Measures Beyond HbA1c for Type 1 Diabetes: A Consensus Report of the American Association of Clinical Endocrinologists, the American Association of Diabetes Educators, the American Diabetes Association, the Endocrine Society, JDRF International, The Leona M. and Harry B. Helmsley Charitable Trust, the Pediatric Endocrine Society, and the T1D Exchange
Authors: Agiostratidou Gina , Anhalt Henry , Ball Dana , Blonde Lawrence , Gourgari Evgenia , Harriman Karen N. , Kowalski Aaron J. , Madden Paul , McAuliffe-Fogarty Alicia H. , McElwee-Malloy Molly , Peters Anne , Raman Sripriya , Reifschneider Kent , Rubin Karen , Weinzimer Stuart A. 
Published: November 2017

OBJECTIVE
To identify and define clinically meaningful type 1 diabetes outcomes beyond hemoglobin A1c (HbA1c) based upon a review of the evidence, consensus from clinical experts, and input from researchers, people with type 1 diabetes, and industry. Priority outcomes include hypoglycemia, hyperglycemia, time in range, diabetic ketoacidosis (DKA), and patient-reported outcomes (PROs). While priority outcomes for type 1 and type 2 diabetes may overlap, type 1 diabetes was the focus of this work.

RESEARCH AND METHODS
A Steering Committee—comprising representatives from the American Association of Clinical Endocrinologists, the American Association of Diabetes Educators, the American Diabetes Association, the Endocrine Society, JDRF International, The Leona M. and Harry B. Helmsley Charitable Trust, the Pediatric Endocrine Society, and the T1D Exchange—was the decision-making body for the Type 1 Diabetes Outcomes Program. Their work was informed by input from researchers, industry, and people with diabetes through Advisory Committees representing each stakeholder group. Stakeholder surveys were used to identify priority outcomes. The outcomes prioritized in the surveys were hypoglycemia, hyperglycemia, time in range, DKA, and PROs. To develop consensus on the definitions of these outcomes, the Steering Committee relied on published evidence, their clinical expertise, and feedback from the Advisory Committees.

RESULTS
The Steering Committee developed definitions for hypoglycemia, hyperglycemia, time in range, and DKA in type 1 diabetes. The definitions reflect their assessment of the outcome’s short- and long-term clinical impact on people with type 1 diabetes. Knowledge gaps to be addressed by future research were identified. The Steering Committee discussed PROs and concluded that further type 1 diabetes–specific development is needed.

CONCLUSIONS
The Steering Committee recommends use of the defined clinically meaningful outcomes beyond HbA1c in the research, development, and evaluation of type 1 diabetes therapies.
Introduction to Modeling Convection in Planets and Stars
Authors: Glatzmaier Gary A. 
Published: November 2017

This book provides readers with the skills they need to write computer codes that simulate convection, internal gravity waves, and magnetic field generation in the interiors and atmospheres of rotating planets and stars. Using a teaching method perfected in the classroom, the book begins by offering a step-by-step guide on how to design codes for simulating nonlinear time-dependent thermal convection in a 2D box using Fourier expansions in the horizontal direction and finite differences in the vertical direction. It then describes how to implement more efficient a	nd accurate numerical methods and more realistic geometries in two and three dimensions. The third part of the book demonstrates how to incorporate more sophisticated physics, including the effects of magnetic field, density stratification, and rotation. The book features numerous exercises throughout, and is an ideal textbook for students and an essential resource for researchers. It explains how to create codes that simulate the internal dynamics of planets and stars, and builds on basic concepts and simple methods. The book shows how to improve the efficiency and accuracy of the numerical methods. It considers more relevant geometries and boundary conditions.
Sudut ATD sebagai Penanda Diabetes Mellitus Tipe-2 (DMT2)
Authors: Syamsurizal Syamsurizal 
Published: November 2017

Pola dermatoglifi (sidik jari) dapat digunakan sebagai penanda genetik DMT2. Analis pola dermatoglifi secara kuantitatif dapat melalui beberapa parameter dermatoglifi antara lain: frekuensi pola sidik jari (arch, loop dan whorl), jumlah sulur, indeks tipe pola (pattern type index) yang terdiri dari indeks Dankmeijer, indeks Furuhata, indeks intensitas pola (pattern intensity index) dan Sudut atd. Sudut atd merupakan sudut yang terbentuk dengan menghubungkan titik triradius a, t dan d pada daerah telapak tangan.Tujuan penelitian menemukan hubungan karakteristik dermatoglifi (sudut atd) dengan diabetes melitus tipe-2 etnis Minangkabau.Metode penelitian yang digunakan deskriptif dengan 132 sampel terdiri dari 66 pasien dengan diabetes melitus tipe-2 dan 66 kontrol. Hasil penelitian tentang sudut atd pada DMT2 adalah 40,44± 3,34 dan pada kontrol 40,42±3,96. Hasil uji-t dan α=5% didapatkan nilai p=0,75. Dengan demikian dapat disimpulkan bahwa tidak ada perbedaan yang signifikan rata-rata sudut atd pada diabetes melitus tipe-2 etnis Minangkabau.Kata kunci: sudut atd, dermataglifi dan DMT2
Meningkatan Kohesivitas Keluarga pada Siswa Remaja Menggunakan Kombinasi Strategi Empty Chair dan Reframing
Authors: Nauli Burju Ruth , Ratnasari Devi , Permatasari Rezky 
Published: November 2017

Kohesivitas keluarga adalah ikatan emosional yang dimiliki anggota keluarga satu terhadap yang lain. Penelitian ini bertujuan untuk menguji keefektifan kombinasi strategi empty chair dan reframing dalam meningkatkan kohesivitas keluarga siswa remaja. Metode penelitian yang digunakan adalah metode kuasi-eksperimen. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kuantitatif untuk mendapatkan data numerikal tentang kohesivitas keluarga pada tiga siswa kelas XII SMA. Hasil penelitian menunjukkan peningkatan setelah diberikan intervensi kombinasi strategi empty dan reframing. Sebagaimana konseli AY skor kohesivitas keluaraga pada baseline 203,00 meningkat menjadi 231,33, konseli YT skor kohesivitas keluarga pada baseline 207,33 meningkat menjadi 240,67, dan konseli PT skor kohesivitas keluaraga pada baseline 211,33 meningkatkan menjadi 239,67.
Musealização da Arqueologia: Diagnóstico do patrimônio arqueológico em museus potiguares
Authors: Silva Abrahão Sanderson Nunes Fernandes da 
Published: November 2017

As coleções arqueológicas quando em contextos museológicos são muitas vezes pouco articuladas com outros conjuntos patrimoniais, revelando relações que foram estabelecidas para com esses bens e evidenciando um processo de isolamento e esquecimento dos objetos arqueológicos enquanto constituintes das memórias locais, regionais ou nacionais. Esta "estratigrafia do abandono" é observada no Rio Grande do Norte a partir dos bens arqueológicos existentes nos museus Câmara Cascudo, Lauro da Escóssia, Seridó e Soledade. Assim, estudando realidades institucionais distintas, busca-se contribuir para a percepção não só do potencial informativo e comunicativo das coleções arqueológicas potiguares encontradas nesses museus, mas também tem-se por objetivo evidenciar a maneira como esse campo do patrimônio cultural brasileiro tem sido abordado no Rio Grande do Norte.
"A persistência da memória”… histórias não-lineares de arqueólogos e foqueiros na Antártica
Authors: Zarankin Andrés 
Published: November 2017

Construir/inventar/interpretar o passado é a função básica do arqueólogo. Refletir sobre este processo se torna, portanto, ponto central da prática arqueológica. Por isso, neste trabalho me proponho discutir algumas ideias, relativas a um tema que considero central e que venho trabalhando ultimamente associadas a como se escrever em arqueologia (ZARANKIN & SEANTORE, 2012). Para isso, vou utilizar como exemplo algumas alternativas que começamos a implementar na pesquisa arqueológica que estamos desenvolvendo na Antártida desde 1995.