bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant herpes simplex virus type 1 strains with targeted mutations relevant for aciclovir susceptibility
Authors: Brunnemann Anne-Kathrin , Liermann Kristin , Deinhardt-Emmer Stefanie , Maschkowitz Gregor , Pohlmann Anja , Sodeik Beate , Fickenscher Helmut , Sauerbrei Andreas , Krumbholz Andi 
Published: July 2016

AbstractHere, we describe a novel reliable method to assess the significance of individual mutations within the thymidine kinase (TK) gene of herpes simplex virus type 1 (HSV-1) to nucleoside analogue resistance. Eleven defined single nucleotide polymorphisms that occur in the TK gene of clinical HSV-1 isolates and a fluorescence reporter were introduced into the HSV-1 strain 17+ that had been cloned into a bacterial artificial chromosome. The susceptibility of these different strains to aciclovir, penciclovir, brivudin, and foscarnet was determined with a modified cytopathic effect reduction assay. The strains were also tested for their aciclovir susceptibility by measuring the relative fluorescence intensity as an indicator for HSV-1 replication and by quantifying the virus yield. Our data indicate that the amino acid substitutions R41H, R106H, A118V, L139V, K219T, S276R, L298R, S345P, and V348I represent natural polymorphisms of the TK protein, whereas G61A and P84L mediate broad cross-resistance against aciclovir, penciclovir, brivudin, and susceptibility to foscarnet. This method allows the definition of the resistance genotype of otherwise unclear mutations in the TK gene of HSV-1. Thus, it provides a scientific basis for antiviral testing in clinical isolates of patients suffering from serious diseases and will facilitate testing of new antivirals against HSV-1.
Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases
Authors: Pei Wuhong , Xu Lisha , Varshney Gaurav K. , Carrington Blake , Bishop Kevin , Jones MaryPat , Huang Sunny C. , Idol Jennifer , Pretorius Pamela R. , Beirl Alisha , Schimmenti Lisa A. , Kindt Katie S. , Sood Raman , Burgess Shawn M. 
Published: July 2016

AbstractPhosphoribosyl pyrophosphate synthetase-1 (PRPS1) is a key enzyme in nucleotide biosynthesis and mutations in PRPS1 are found in several human diseases including nonsyndromic sensorineural deafness, Charcot-Marie-Tooth disease-5 and Arts Syndrome. We utilized zebrafish as a model to confirm that mutations in PRPS1 result in phenotypic deficiencies in zebrafish similar to those in the associated human diseases. We found two paralogs in zebrafish, prps1a and prps1b and characterized each paralogous mutant individually as well as the double mutant fish. Zebrafish prps1a mutants and prps1a;prps1b double mutants showed similar morphological phenotypes with increasingly severe phenotypes as the number of mutant alleles increased. Phenotypes included smaller eyes and reduced hair cell numbers, consistent with the optic atrophy and hearing impairment observed in human patients. The double mutant also showed abnormal development of primary motor neurons, hair cell innervation and reduced leukocytes, consistent with the neuropathy and recurrent infection of the human patients possessing the most severe reductions of PRPS1 activity. Further analyses indicated the phenotypes were associated with a prolonged cell cycle likely resulting from reduced nucleotide synthesis and energy production in the mutant embryos. We further demonstrated the phenotypes were caused by delays in the tissues most highly expressing the prps1 genes.
The acquisition of Clostridium tyrobutyricum mutants with improved bioproduction under acidic conditions after two rounds of heavy-ion beam irradiation
Authors: Zhou Xiang , Yang Zhen , Jiang Ting-Ting , Wang Shu-Yang , Liang Jian-Ping , Lu Xi-Hong , Wang Liang 
Published: July 2016

AbstractEnd-product inhibition is a key factor limiting the production of organic acid during fermentation. Two rounds of heavy-ion beam irradiation may be an inexpensive, indispensable and reliable approach to increase the production of butyric acid during industrial fermentation processes. However, studies of the application of heavy ion radiation for butyric acid fermentation engineering are lacking. In this study, a second 12C6+ heavy-ion irradiation-response curve is used to describe the effect of exposure to a given dose of heavy ions on mutant strains of Clostridium tyrobutyricum. Versatile statistical elements are introduced to characterize the mechanism and factors contributing to improved butyric acid production and enhanced acid tolerance in adapted mutant strains harvested from the fermentations. We characterized the physiological properties of the strains over a large pH value gradient, which revealed that the mutant strains obtained after a second round of radiation exposure were most resistant to harsh external pH values and were better able to tolerate external pH values between 4.5 and 5.0. A customized second round of heavy-ion beam irradiation may be invaluable in process engineering.
CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle
Authors: Steinbach Sabine , Vordermeier H. Martin , Jones Gareth J. 
Published: July 2016

AbstractGene transcription studies have identified dual roles for the cytokines IL-17A and IL-22 in bovine tuberculosis, where they show potential as both predictors of vaccine success and correlates of infection. To allow for a detailed investigation of the cell populations responsible for production of these cytokines, we have utilised a novel bovine IL-22 specific recombinant antibody for flow cytometry. Bovine tuberculin (PPDB) induced greater IL-22 and IL-17A production in Mycobacterium bovis (M. bovis)-infected cattle compared to non-infected controls, while PWM-induced cytokine levels were similar between the two groups. In M. bovis-infected animals, PPDB specific IL-22 and IL-17A responses were observed in both CD4+ T cell and γδ T cell populations. Although both cytokines were detected in both cell types, IL-22/IL-17A double producers were rare and confined mainly to the γδ T cell population. These results support previous gene transcription studies and extend the observation of increased IL-22 and IL-17A responses in M. bovis-infected animals to the level of protein production. We were also able to characterise the cell populations responsible for these disease-related cytokine responses. The data generated can be used to further our understanding of the immunopathology of bovine tuberculosis and to produce more sensitive and specific immune-diagnostic reagents.
Wetting length in gas metal arc brazing of galvanised steel
Authors: Makwana P. , Shome M. , Goecke S.-F. , De A. 
Published: July 2016

Appropriate substrate surface wetting by a molten filler ensures a sound bead profile during brazing. A theory to estimate the wetting length considering gradual cooling of liquid filler and loss in its ability to spread is currently unavailable. We present here a methodology to estimate wetting length based on the minimisation of energy for spreading of a liquid filler considering its gradual cooling and tested the computed results in gas metal arc brazing of galvanised sheets for a wide range of conditions. The computed wetting lengths are used further to estimate bead height and toe angle of joint profile. GRAPHICAL ABSTRACT
StableC. eleganschromatin domains separate broadly expressed and developmentally regulated genes
Authors: Evans Kenneth J. , Huang Ni , Stempor Przemyslaw , Chesney Michael A. , Down Thomas A. , Ahringer Julie 
Published: July 2016

AbstractEukaryotic genomes are organized into domains of differing structure and activity. There is evidence that the domain organization of the genome regulates its activity, yet our understanding of domain properties and the factors that influence their formation is poor. Here we use chromatin state analyses in early embryos and L3 larvae to investigate genome domain organization and its regulation inC. elegans. At both stages we find that the genome is organized into extended chromatin domains of high or low gene activity defined by different subsets of states, and enriched for H3K36me3 or H3K27me3 respectively. The border regions between domains contain large intergenic regions and a high density of transcription factor binding, suggesting a role for transcription regulation in separating chromatin domains. Despite the differences in cell types, overall domain organization is remarkably similar in early embryos and L3 larvae, with conservation of 85% of domain border positions. Most genes in high activity domains are expressed in the germ line and broadly across cell types, whereas low activity domains are enriched for genes that are developmentally regulated. We find that domains are regulated by the germ line H3K36 methyltransferase MES-4 and that border regions show striking remodeling of H3K27me1, supporting roles for H3K36 and H3K27 methylation in regulating domain structure. Our analyses ofC. eleganschromatin domain structure show that genes are organized by type into domains that have differing modes of regulation.Significance statementGenomes are organized into domains of different structure and activity, yet our understanding of their formation and regulation is poor. We show thatC. eleganschromatin domain organization in early embryos and L3 larvae is remarkably similar despite the two developmental stages containing very different cell types. Chromatin domains separate genes into those with stable versus developmentally regulated expression. Analyses of chromatin domain structure suggest that transcription regulation and germ line chromatin regulation play roles in separating chromatin domains. Our results further our understanding of genome domain organization.
Performance of semi-automated hippocampal subfield segmentation methods across ages in a pediatric sample
Authors: Schlichting Margaret L. , Mack Michael L. , Guarino Katharine F. , Preston Alison R. 
Published: July 2016

ABSTRACTEpisodic memory function has been shown to depend critically on the hippocampus. This region is made up of a number of subfields, which differ in both cytoarchitectural features and functional roles in the mature brain. Recent neuroimaging work in children and adolescents has suggested that these regions may undergo different developmental trajectories—a fact that has important implications for how we think about learning and memory processes in these populations. Despite the growing research interest in hippocampal structure and function at the subfield level in healthy young adults, comparatively fewer studies have been carried out looking at subfield development. One barrier to studying these questions has been that manual segmentation of hippocampal subfields—considered by many to be the best available approach for defining these regions—is laborious and can be infeasible for large cross-sectional or longitudinal studies of cognitive development. Moreover, manual segmentation requires some subjectivity and is not impervious to bias or error. In a developmental sample of individuals spanning 6-30 years, we assessed the degree to which two semi-automated segmentation approaches—one approach based on Automated Segmentation of Hippocampal Subfields (ASHS) and another utilizing Advanced Normalization Tools (ANTs)—approximated manual subfield delineation on each individual by a single expert rater. Our main question was whether performance varied as a function of age group. Across several quantitative metrics, we found negligible differences in subfield validity across the child, adolescent, and adult age groups, suggesting that these methods can be reliably applied to developmental studies. We conclude that ASHS outperforms ANTs overall and is thus preferable for analyses carried out in individual subject space. However, we underscore that ANTs is also acceptable and may be well-suited for analyses requiring normalization to a single group template (e.g., voxelwise analyses across a wide age range). Previous work has supported the use of such methods in healthy young adults, as well as several special populations such as older adults and those suffering from mild cognitive impairment. Our results extend these previous findings to show that ASHS and ANTs can also be used in pediatric populations as young as six.
Tradition and innovation in Italian wine family businesses
Authors: Vrontis Demetris , Bresciani Stefano , Giacosa Elisa 
Published: July 2016

Purpose
– The purpose of this paper is to analyse how a strategy based on the combination of tradition and innovation can offer a competitive advantage to a medium-sized family firm operating in the wine sector.

Design/methodology/approach
– This research focuses on one case study. The subject of the case study under analysis is Pio Cesare, an internationally recognized fine wine Italian company.

Findings
– Pio Cesare is characterized by a strong combination of tradition and innovation in terms of products and processes. Innovation and tradition are not opposites; on the contrary, a blend of the two has been crucial in achieving and maintaining a sustainable competitive advantage.

Research limitations/implications
– The formalization of an effective blend of tradition and innovation, especially in a family business, and an effective dissemination of the company philosophy to future generations, calls for a more in-depth study. This study has some limitations, which determined by the applied methodology. Multiple case study method would be useful. In relation to the interview technique, the authors would set out to increase the number of interviews, by also questioning managers and employees working, as opposed to family members.

Practical implications
– Practical implications relate to different groups of stakeholders: for owners and management, for investors, for organizations and institutions working on a territory promotion and in the tourism sector, and for politicians and local authorities.

Originality/value
– It identifies which business model a family winery business could be adopted in order to manage such strategies of innovation in respect to traditions, filling the gap in the literature.
Competitiveness of short sea shipping: the case of olive oil industry
Authors: Galati Antonino , Siggia Dario , Crescimanno Maria , Martín-Alcalde Enrique , Saurí Marchán Sergi , Morales-Fusco Pau 
Published: July 2016

Purpose– The purpose of this paper is to analyse the economic benefits of short sea shipping (SSS) in the shape of Motorways of the Sea (MoS) compared to road transport. The study cover a gap in agro-food economics and analyses the economic benefits of sea transport mode compared to road transport in the food trade between Spain and Italy for a specific product: olive oils.Design/methodology/approach– Three different transportation scenarios are considered (road only, road combined with accompanied SSS and road combined with unaccompanied SSS) linking the main olive oil production and consumption areas in Spain and Italy. In each scenario the cost per unit shipped have been calculated.Findings– The results show the road option is about 30 and 34 per cent more costly than the best SSS option available for the exportations from Jaén and Southern Catalonia, respectively.Research limitations/implications– The need of further research is identified, mainly focused in two directions: first, the need for inclusion of new variables to the model (e.g. value of time, seasonality, complexity of the transport chain, potential demand, etc.) to better assess the competitiveness of the sea connection and, second, a study of the environmental impact and socio-economic benefits of SSS implementation for the agri-food sector.Originality/value– The research enriches the current literature on this field and provides a basis for future studies. In particular, it corroborates the strategic decisions taken in the framework of European transport policy demonstrating a greater economic sustainability of SSS, and more specifically MoS, compared to the road transport.
Status or fun? A cross-cultural examination of young consumers’ responses to images of champagne and sparkling wine
Authors: Velikova Natalia , Charters Steve , Fountain Joanna , Ritchie Caroline , Fish Nicola , Dodd Tim 
Published: July 2016

Purpose– The purpose of this paper is to test Luna and Gupta’s (2001) investigative framework on the interaction of cultural values and consumer behaviour by conducting a cross-cultural comparison of young wine consumers’ interpretation of images of champagne and sparkling wine. The research examined consumer responses to the images through the prism of the relationship between symbolism, ritual and myth, as well as other related values.Design/methodology/approach– In a series of focus groups with consumers from four anglophone countries (the USA, New Zealand, Australia and the UK), six images of champagne and sparkling wine were used as stimuli to encourage affective and cognitive perspectives on the topic.Findings– Overall, the UK market showed distinct differences from the other markets, due very much to its cultural context. The UK consumers valued traditional advertising; focused mainly on the product itself; and did not associate champagne with fun. Respondents from the New World focused on the general impression of the image and on enjoyment and fun associated with consumption of champagne and sparkling wine.Practical implications– The most crucial implication of this research is the cultural variation in consumer perceptions of champagne and sparkling wine and the impact that it has upon marketing strategies on how to market this product category to younger consumers in different markets.Originality/value– This research contributes to the study of cultural values and consumption behaviour, as well as image effectiveness in forming perceptions of the product category.
Unified approach for modeling innovation adoption and optimal model selection for the diffusion process
Authors: Anand Adarsh , Agarwal Mohini , Aggrawal Deepti , Singh Ompal 
Published: July 2016

Purpose
– Mathematical modeling of innovation diffusion is a constantly evolving field within marketing science. The diffusion process explains the dispersion of an innovation among potential buyers. Prior research on innovation diffusion has been based on modeling varied aspects of real life situations in marketing. One such aspect is studying the adoption process depending on the awareness and motivation level among the customers. Awareness is having knowledge of an innovation, whereas motivation is about the perception of an individual. In line with these aspects, the purpose of this paper is to propose a unified modeling framework for the adoption process based on the awareness and motivation about the product.

Design/methodology/approach
– When the market is well informed about the product, there are some people who are motivated and some, who have adopted the product earlier and shall now influence others in their buying behavior. It is very much similar to queuing system in which some units are waiting in a queue for the service, service for some units are being processed and some units have already been served. This analogous behavior between two approaches has motivated the use of infinite server queuing theory in modeling adoption of the product. Thereafter, the authors have proposed a unification scheme to model different market scenarios.

Findings
– From analyzing the values of comparison criteria, it was not clear that which among them is performing best. Thus there was a need for an approach which can judiciously find the optimal model. For this very purpose the authors applied distance-based approach which was capable of computing the optimal model based on the distance of attribute value from the optimal. The analysis performed on two real life sales data sets depict that model in which awareness is following logistic pattern and motivation and adoption are following a constant pattern is ranked one.

Research limitations/implications
– The idea has been validated on product. It would be interesting to know how the methodology works on service.

Originality/value
– The modeling framework discussed in this paper can be helpful to know from the available set of alternative, which among them is performing better in capturing the spread of the product in the market. The proposed framework offer some managerial guidance by highlighting the unusual aspects of diffusion process and also present an approach to judge the best among a set of different models.
WTE plants installed in European cities: a review of success stories
Authors: Chaliki Paraskevi , Psomopoulos Constantinos S. , Themelis Nickolas J. 
Published: July 2016

Purpose
– Waste is a resource. Generating energy from waste instead of sending it to landfill avoids methane gas which equals 25 times CO2 in mass. In combination with the energy efficiency thresholds set in Waste Framework Directive, this could prevent up to a further 45 million tons of CO2 eq. per year. The purpose of this paper is to present the waste-to-energy (WTE) plants installed in ten European cities which have been selected among the most sustainable cities or among the best cities to live in.

Design/methodology/approach
– The work is based on literature review and a combination of several statistical data and reports that include the required data.

Findings
– The European Directives, along with the general thinking that wastes are resources and the effort to reduce the environmental impact in urban environment from waste management, were the driving forces. The most sustainable cities in EU considered that their sustainability is based also in energy recovery from wastes. All of them are using WTE facilities to treat a significant part of their waste in order to produce energy in the form of heat and electricity. And they do it in a very successful and environmental friendly way, as they mainly utilize the waste fractions that cannot be recycled or reused, and they do not landfill these resources. This approach is proving that the sustainable waste management cannot be achieved without WTE facilities, since a fraction of wastes consists of non-recyclable and non-reusable materials, which present significant heating value that cannot be neglected as an energy source.

Originality/value
– This paper presents the WTE plants installed in ten European cities which have been selected among the most sustainable cities or among the best cities to live in. This work aims to present the strong and successful relation between WTE and sustainability in the modern complex urban environment.