bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production, amplification and systemic propagation of redox messengers in plants? The phloem can do it all!
Authors: Gaupels Frank , Durner Jörg , Kogel Karl‐Heinz 
Published: January 2017

SummaryRapid long‐distance signalling is an emerging topic in plant research, and is particularly associated with responses to biotic and abiotic stress. Systemic acquired resistance (SAR) to pathogen attack is dependent on nitric oxide (NO) and reactive oxygen species (ROS) such as hydrogen peroxide (H2O2). By comparison, systemic wound responses (SWRs) and systemic acquired acclimation (SAA) to abiotic stress encounters are triggered by rapid waves of H2O2, calcium and electrical signalling. Efforts have been made to decipher the relationship between redox messengers, calcium and other known systemic defence signals. Less is known about possible routes of signal transduction throughout the entire plant. Previously, the phloem has been suggested to be a transport conduit for mobile signals inducing SAR, SWR and SAA. This review highlights the role of the phloem in systemic redox signalling by NO and ROS. A not yet identified calcium‐dependent NO source and S‐nitrosoglutathione reductase are candidate regulators of NO homeostasis in the phloem, whereas ROS concentrations are controlled by NADPH oxidases and the H2O2‐scavenging enzyme ascorbate peroxidase. Possible amplification mechanisms in phloem‐mediated systemic redox signalling are discussed.
Allelic variations and differential expressions detected at quantitative trait loci for salt stress tolerance in wheat
Authors: Oyiga Benedict C. , Sharma Ram C. , Baum Michael , Ogbonnaya Francis C. , Léon Jens , Ballvora Agim 
Published: January 2017

AbstractThe increasing salinization of agricultural lands is a threat to global wheat production. Understanding of the mechanistic basis of salt tolerance (ST) is essential for developing breeding and selection strategies that would allow for increased wheat production under saline conditions to meet the increasing global demand. We used a set that consists of 150 internationally derived winter and facultative wheat cultivars genotyped with a 90K SNP chip and phenotyped for ST across three growth stages and for ionic (leaf K+ and Na+ contents) traits to dissect the genetic architecture regulating ST in wheat. Genome‐wide association mapping revealed 187 Single Nucleotide Polymorphism (SNPs) (R2 = 3.00–30.67%), representing 37 quantitative trait loci (QTL), significantly associated with the ST traits. Of these, four QTL on 1BS, 2AL, 2BS and 3AL were associated with ST across the three growth stages and with the ionic traits. Novel QTL were also detected on 1BS and 1DL. Candidate genes linked to these polymorphisms were uncovered, and expression analyses were performed and validated on them under saline and non‐saline conditions using transcriptomics and qRT‐PCR data. Expressed sequence comparisons in contrasting ST wheat genotypes identified several non‐synonymous/missense mutation sites that are contributory to the ST trait variations, indicating the biological relevance of these polymorphisms that can be exploited in breeding for ST in wheat.
Mechanistic studies of sesquiterpene cyclases based on their carbon isotope ratios at natural abundance
Authors: Tan Wenhua , Bartram Stefan , Boland Wilhelm 
Published: January 2017

AbstractDuring the process of terpene biosynthesis, C–C bond breaking and forming steps are subjected to kinetic carbon isotope effects, leading to distinct carbon isotopic signatures of the products. Accordingly, carbon isotopic signatures could be used to reveal the ‘biosynthetic history’ of the produced terpenoids. Five known sesquiterpene cyclases, regulating three different pathways, representing simple to complex biosynthetic sequences, were heterologously expressed and used for in vitro assays with farnesyl diphosphate as substrate. Compound specific isotope ratio mass spectrometry measurements of the enzyme substrate farnesyl diphosphate (FDP) and the products of all the five cyclases were performed. The calculated δ13C value for FDP, based on δ13C values and relative amounts of the products, was identical with its measured δ13C value, confirming the reliability of the approach and the precision of measurements. The different carbon isotope ratios of the products reflect the complexity of their structure and are correlated with the frequency of carbon–carbon bond forming and breaking steps on their individual biosynthetic pathways. Thus, the analysis of carbon isotopic signatures of terpenes at natural abundance can be used as a powerful tool in elucidation of associated biosynthetic mechanisms of terpene synthases and in future in vivo studies even without ‘touching’ the plant.
Cataracts in the Norwegian Buhund—current prevalence and characteristics
Authors: Kristiansen Elin , Revold Tobias , Lingaas Frode , Narfström Kristina , Pedersen Pia Bjerre , Kielland Camilla , Dahl Stein , Ropstad Ernst‐Otto 
Published: January 2017

AbstractObjectiveTo evaluate prevalence and characteristics of cataracts in the Norwegian Buhund breed 20 years after high reported prevalence of especially pulverulent nuclear cataracts (PNCs).Animals studiedTwo hundred and fifty Norwegian Buhund dogs in Norway, Sweden, and Denmark (117 males and 133 females) with previously unknown eye health status were included. Forty‐five dogs had multiple examinations (two to six times over a 6‐year period). Median age was 4.4 years [0.2–15.2] at first examination and 5.3 years [0.2–15.2] at last examination.ProceduresAll dogs underwent regular screening for inherited eye diseases.ResultsAt the last observation of each dog, 52.4% were affected by PNC, categorized as minimal (33 of 250 dogs; 13.2%), mild (31 dogs; 12.4%), moderate (38 dogs; 15.2%), or pronounced (29 dogs; 11.6%). Moderate or pronounced changes were only seen in older dogs, and progressive changes were identified in some of the re‐examined dogs. Some dogs, free of lenticular changes at early examinations, were affected by PNC at re‐examinations. The odds for finding PNC increased with dog's age up to approximately 8 years. Presumably inherited cataracts other than PNC were found in 53 dogs (21.2%) with cortical (17.6%) and posterior polar (6.4%) locations as the most common ones.ConclusionsThe high prevalence of PNC in the breed reported 20 years ago persists. PNCs are not always visible in young dogs, and the rate of progression varies. The prevalence of other types of cataract is also high, but cataracts rarely cause loss of vision in this breed.
Effects of hypercapnia on the lung
Authors: Shigemura Masahiko , Lecuona Emilia , Sznajder Jacob I. 
Published: January 2017

AbstractGases are sensed by lung cells and can activate specific intracellular signalling pathways, and thus have physiological and pathophysiological effects. Carbon dioxide (CO2), a primary product of oxidative metabolism, can be sensed by eukaryotic cells eliciting specific responses via recently identified signalling pathways. However, the physiological and pathophysiological effects of high CO2 (hypercapnia) on the lungs and specific lung cells, which are the primary site of CO2 elimination, are incompletely understood. In this review, we provide a physiological and mechanistic perspective on the effects of hypercapnia on the lungs and discuss the recent understanding of CO2 modulation of the alveolar epithelial function (lung oedema clearance), epithelial cell repair, innate immunity and airway function.
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Draft Genome Sequences of 40 Pseudomonas aeruginosa Clinical Strains Isolated from the Sputum of a Single Cystic Fibrosis Patient Over an 8-Year Period
Authors: Bianconi Irene , D'Arcangelo Silvia , Benedet Mattia , Bailey Kate E. , Esposito Alfonso , Piffer Elena , Mariotto Alex , Baldo Ermanno , Dinnella Grazia , Gualdi Paola , Schinella Michele , Donati Claudio , Jousson Olivier 
Published: January 2017

ABSTRACT

We report draft genome sequences of 40
Pseudomonas aeruginosa
strains, isolated from the sputum of a single cystic fibrosis patient over eight years. Analyses indicated a correlation between multidrug-resistant phenotypes and population structure. Our data provide new insights into the mechanisms leading to acquisition of antibiotic resistance in
P. aeruginosa
.
Draft Genome Sequences of Itaconate-Producing
Ustilaginaceae
Authors: Geiser Elena , Ludwig Florian , Zambanini Thiemo , Wierckx Nick , Blank Lars M. 
Published: January 2017

ABSTRACT

Some smut fungi of the family
Ustilaginaceae
produce itaconate from glucose.
De novo
genome sequencing of nine itaconate-producing
Ustilaginaceae
revealed genome sizes between 19 and 25 Mbp. Comparison to the itaconate cluster of
U. maydis
MB215 revealed all essential genes for itaconate production contributing to metabolic engineering for improving itaconate production.
Complete Genome Sequences of Curli-Negative and Curli-Positive Isolates of Foodborne Escherichia coli O157:H7 Strain 86-24
Authors: Sharma Vijay K. , Bayles Darrell O. , Alt David P. , Looft Torey 
Published: January 2017

ABSTRACT

Escherichia coli
O157:H7 strain 86-24 does not produce curli fimbriae, but gives rise to curli-positive isolates at a variable frequency. Here, we report the complete genome sequences of curli-negative and curli-positive isolates of strain 86-24.
Complete Genome Sequence of Type Strain Campylobacter fetus subsp.
fetus
ATCC 27374
Authors: Oliveira Luciana M. , Resende Daniela M. , Dorneles Elaine M. S. , Horácio Elvira C. A. , Alves Fernanda L. , Gonçalves Leilane O. , Tavares Grace S. , Stynen Ana Paula R. , Lage Andrey P. , Ruiz Jeronimo C. 
Published: January 2017

ABSTRACT

Campylobacter fetus
subsp.
fetus
is a zoonotic bacterium important for animal and public health. The complete sequencing and annotation of the genome of the type strain
C. fetus
subsp.
fetus
ATCC 27374 are reported here.
Draft Genome Sequence of Bacillus subtilis Ia1a, a New Strain for Poly-γ-Glutamic Acid and Exopolysaccharide Production
Authors: Ibrahim Mohammad H. A. , Cress Brady F. , Linhardt Robert J. , Koffas Mattheos A. G. , Gross Richard A. 
Published: January 2017

ABSTRACT

We report here the 4.092-Mb high-quality draft genome assembly of a newly isolated poly-γ-glutamic acid–producing strain,
Bacillus subtilis
Ia1a. The genome sequence is considered a critical tool to facilitate the engineering of improved production strains. Exopolysaccharides and many industrially important enzymes can be produced by this new strain utilizing different carbon sources.
Draft Genome Sequence of Pediococcus parvulus 2.6, a Probiotic β-Glucan Producer Strain
Authors: Pérez-Ramos Adrián , Mohedano M. Luz , Puertas Ana , Lamontanara Antonella , Orru Luigi , Spano Giuseppe , Capozzi Vittorio , Dueñas M. Teresa , López Paloma 
Published: January 2017

ABSTRACT

We report here the draft genome sequence of the probiotic
Pediococcus parvulus
2.6, a lactic acid bacterial strain isolated from ropy cider. The bacterium produces a prebiotic and immunomodulatory exopolysaccharide, and this is the first strain of the
P. parvulus
species whose genome has been characterized.
Draft Genome Sequence of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strain CCUG 62462, Isolated from a Urine Sample
Authors: Johnning Anna , Jakobsson Hedvig E. , Boulund Fredrik , Salvà-Serra Francisco , Moore Edward R. B. , Åhrén Christina , Karami Nahid , Kristiansson Erik 
Published: January 2017

ABSTRACT

The draft genome sequence has been determined for an extended-spectrum-β-lactamase (ESBL)-producing (
bla
CTX-M-15
)
Escherichia coli
strain (CCUG 62462), composed of 119 contigs and a total size of 5.27 Mb. This
E. coli
is serotype O25b and sequence type 131, a pandemic clonal group, causing worldwide antimicrobial-resistant infections.
Draft Genome Sequence of Blautia faecis Strain Marseille-P328, Isolated from the Human Ascending Colon
Authors: Ricaboni Davide , Mailhe Morgane , Labas Noémie , Vitton Véronique , Raoult Didier , Million Matthieu 
Published: January 2017

ABSTRACT

Blautia faecis
strain Marseille P328 was isolated from the ascending colon of a French patient. We sequenced the 4.45-Mb genome of the strain and compared it with that of other species of the
Blautia
genus.
Draft Genome Sequence of Enterococcus faecalis Strain UCD-PD3
Authors: De Vries Dana R. , Martin Alexandra L. , Ganz Holly H. , Eisen Jonathan A. , Coil David A. 
Published: January 2017

ABSTRACT

Here, we present the draft genome sequence of
Enterococcus faecalis
strain UCD-PD3. The assembly contains 2,861,314 bp in 73 contigs. This strain was isolated from a feral domestic cat (
Felis catus
) anal sac secretion sample, as part of a project on isolating and characterizing the microbes present in feline anal sacs.
Genome Sequence of
Rheinheimera salexigens
sp. nov. Isolated from a Fishing Hook off O‘ahu, Hawai‘i
Authors: Wan Xuehua , Hou Shaobin , Hayashi Kazukuni , Anderson James , Donachie Stuart P. 
Published: January 2017

ABSTRACT

Rheinheimera salexigens
KH87
T
is an obligately halophilic gammaproteobacterium. The strain's draft genome sequence, generated by the Roche 454 GS FLX+ platform, comprises two scaffolds of ~3.4 Mbp and ~3 kbp, with 3,030 protein-coding sequences and 58 tRNA coding regions. The G+C content is 42 mol%.
High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies
Authors: Buchmann Anina , Eitel Michael , Koch Pierre , Schwarz Paul N. , Stegmann Evi , Wohlleben Wolfgang , Wolański Marcin , Krawiec Michał , Zakrzewska-Czerwińska Jolanta , Méndez Carmen , Botas Alma , Núñez Luz Elena , Morís Francisco , Cortés Jesus , Gross Harald 
Published: January 2017

ABSTRACT

The bacterium
Nocardia terpenica
IFM 0406 is known as the producer of the immunosuppressant brasilicardin A. Here, we report the completely sequenced genome of strain IFM 0406, which facilitates the heterologous expression of the brasilicardin biosynthetic gene cluster but also unveils the intriguing biosynthetic capacity of the strain to produce secondary metabolites.
Complete Genome Sequence of Delftia tsuruhatensis CM13 Isolated from Murine Proximal Colonic Tissue
Authors: Saffarian Azadeh , Mulet Céline , Tournebize Régis , Naito Tomoaki , Sansonetti Philippe J. , Pédron Thierry 
Published: January 2017

ABSTRACT

We report here the complete genome sequence of
Delftia tsuruhatensis
CM13, isolated from murine proximal colonic tissue. The genome assembly using PacBio single-molecule real-time sequencing resulted in a single scaffold of 7.19 Mb.
Genome Sequence of the Poly-3-Hydroxybutyrate Producer Clostridium acetireducens DSM 10703
Authors: Flüchter Sebastian , Poehlein Anja , Schiel-Bengelsdorf Bettina , Daniel Rolf , Dürre Peter 
Published: January 2017

ABSTRACT

Here, we report the genome sequence of
Clostridium acetireducens
(DSM 10703
T
), a strictly anaerobic bacterium capable of fermenting acetate and leucine to butyrate, isovalerate, and poly-3-hydroxybutyrate. The draft genome consists of a circular chromosome with a size of 2.4 Mb and harbors 2,239 predicted protein-encoding genes.
Genome Sequences of Multidrug-Resistant Salmonella enterica subsp.
enterica
Serovar Infantis Strains from Broiler Chicks in Hungary
Authors: Wilk Tímea , Szabó Móni , Szmolka Ama , Kiss János , Barta Endre , Nagy Tibor , Olasz Ferenc , Nagy Béla 
Published: January 2017

ABSTRACT

Three strains of
Salmonella enterica
serovar Infantis isolated from healthy broiler chickens from 2012 to 2013 have been sequenced. Comparison of these and previously published
S
. Infantis genome sequences of broiler origin in 1996 and 2004 will provide new insight into the genome evolution and recent spread of
S
. Infantis in poultry.
Complete Genome Sequence of Community-Acquired Klebsiella pneumoniae KP36, a Strain Isolated from a Patient with an Upper Urinary Tract Infection
Authors: Lin Wei-Hung , Zheng Po-Xing , Liu Tsunglin , Tseng Chin-Chung , Chen Wei-Chu , Wang Ming-Cheng , Wu Jiunn-Jong 
Published: January 2017

ABSTRACT

Here, we announce the complete genome sequence of
Klebsiella pneumoniae
KP36, a strain isolated from a patient with a severe community-acquired urinary tract infection. This genome provides insights into the pathogenesis of a pandemic
K. pneumoniae
strain from a community-acquired urinary tract infection.
Complete Genome Sequence of
Rhodococcus
sp. Strain WMMA185, a Marine Sponge-Associated Bacterium
Authors: Adnani Navid , Braun Doug R. , McDonald Bradon R. , Chevrette Marc G. , Currie Cameron R. , Bugni Tim S. 
Published: January 2017

ABSTRACT

The
Rhodococcus
strain WMMA185 was isolated from the marine sponge
Chondrilla nucula
as part of ongoing drug discovery efforts. Analysis of the 4.44-Mb genome provides information regarding interspecies interactions as pertains to regulation of secondary metabolism and natural product biosynthetic potentials.
Genome Sequence of “
Anthococcus
,” a Novel Genus of the Family
Streptococcaceae
Isolated from Flowers
Authors: Chuah Li-Oon , Yap Kien-Pong , Thong Kwai Lin , Liong Min Tze , Ahmad Rosma , Shamila-Syuhada Ahamed Kamal , Rusul Gulam 
Published: January 2017

ABSTRACT

Here, we report the draft whole-genome sequence of “
Anthococcus
,” a novel genus of the family
Streptococcaceae
isolated from fresh flowers of a durian (
Durio zibethinus
) tree. The draft genome of
Anthococcus
sp. strain DF1 contains 2,157,756 bp, with a G+C content of 33.0%.
Genome Sequences of Multidrug-Resistant, Colistin-Susceptible and -Resistant Klebsiella pneumoniae Clinical Isolates from Pakistan
Authors: Crawford Matthew A. , Timme Ruth , Lomonaco Sara , Lascols Christine , Fisher Debra J. , Sharma Shashi K. , Strain Errol , Allard Marc W. , Brown Eric W. , McFarland Melinda A. , Croley Tim , Hammack Thomas S. , Weigel Linda M. , Anderson Kevin , Hodge David R. , Pillai Segaran P. , Morse Stephen A. , Khan Erum , Hughes Molly A. 
Published: January 2017

ABSTRACT

The emergence and spread of colistin resistance among multidrug-resistant (MDR)
Klebsiella pneumoniae
represent a critical threat to global health. Here, we report the complete genome sequences of 10 MDR, colistin-susceptible and -resistant
K. pneumoniae
clinical isolates obtained in Pakistan between 2010 and 2013.
Draft Genome Sequences of Type Strains Bacillus drentensis DSM 15600
T
and Bacillus novalis DSM 15603
T
Authors: Liu Bo , Liu Guo-Hong , Zhu Yu-jing , Wang Jie-Ping , Che Jian-Mei , Chen Qian-Qian , Chen Zheng 
Published: January 2017

ABSTRACT

Here, we report the draft genome sequences of
Bacillus drentensis
DSM 15600
T
and
Bacillus novalis
DSM 15603
T
with 5,305,306 bp and 5,667,584 bp, respectively, which will provide useful information for the functional gene mining and application of these two species. The average DNA G+C contents were 38.91% and 40.01%, respectively.
Racecadotril for the treatment of severe acute watery diarrhoea in children admitted to a tertiary hospital in Kenya
Authors: Gharial Jaspreet , Laving Ahmed , Were Fred 
Published: January 2017

BackgroundDiarrhoea is the second most common cause of death in children under 5 years of age in Kenya. It is usually treated with oral rehydration, zinc and continued feeding. Racecadotril has been in use for over 2 decades; however, there is a paucity of data regarding its efficacy from Africa.ObjectivesThe objectives of this study were: to compare the number of stools in the first 48 hours in children with severe gastroenteritis requiring admission and treated with either racecadotril or placebo, to study the impact of racecadotril on duration of inpatient stay as well as duration of diarrhoea and to describe the side effect profile of racecadotril.MethodsThis was a randomised, double-blinded, placebo-controlled trial. It enrolled children between the age of 3 and 60 months who were admitted with severe acute gastroenteritis. They received either racecadotril or placebo in addition to oral rehydration solution (ORS) and zinc and were followed up daily.Results120 children were enrolled into the study. There were no differences in the demographics or outcomes between the 2 groups. Stools at 48 hours: median (IQR) of 5 (3–7) and 5 (2.5–7.5), respectively; p=0.63. The duration of inpatient stay: median (IQR): 4 days (1.5–6.5) and 4.5 (1.8–6.3); p=0.71. The duration of illness: 3 days (2–4) and 2 days (1–3); p=0.77. The relative risk of a severe adverse event was 3-fold higher in the drug group but was not statistically significant (95% CI 0.63 to 14.7); p=0.16.ConclusionsRacecadotril has no impact on the number of stools at 48 hours, the duration of hospital stay or the duration of diarrhoea in children admitted with severe gastroenteritis and managed with ORS and zinc.Trial registration numberPACTR201403000694398; Pre-results.
PPH Butterfly: a novel device to treat postpartum haemorrhage through uterine compression
Authors: Cunningham Caroline , Watt Peter , Aflaifel Nasreen , Collins Simon , Lambert Dot , Porter John , Lavender Tina , Fisher Tony , Weeks Andrew 
Published: January 2017

ObjectivePostpartum haemorrhage (PPH) is a significant cause of maternal morbidity and mortality. The most common cause is an inability of the uterus to contract adequately after childbirth. In bimanual compression (BMC), one hand is placed within the vagina and the other hand is on the abdominal wall to compress the uterus. It is effective, but very uncomfortable for the woman. We designed a device that could replicate BMC without inserting a hand vaginally, therefore being less invasive. It could also help in diagnosing the source of the bleeding.DesignMixed methods, combining an iterative design process with input from clinicians in simulations, and focus groups of clinicians and consumers.SettingDepartment of Women's and Children's Health and Department of Medical Physics and Clinical Engineering, University of Liverpool, UK.MethodsA multidisciplinary team developed the design, using an obstetric manikin. Clinician and consumer groups also gave input on the concept and design. A healthcare product company and prototype manufacturer provided input into strategy, design and manufacture.ResultsThe PPH Butterfly is a single piece, plastic medical device that replicates BMC. It is designed to be easy to use and low-cost and allows for smooth insertion and removal. It is acceptable to clinicians and consumers and performs well in tests.ConclusionsThis is the first device designed to replicate BMC while being less invasive. It could potentially be an effective form of PPH management, while also diagnosing the source of the bleeding. The device will now be tested in humans.