bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compostaje de estiércol de feedlot con aserrín/viruta: características del proceso y del producto final
Authors: Hang S. , Castán E. , Daghero A. , Buffa E. , Ringuelet A. , Satti P. , Mazzarino M.J. , Negro G. 
Published: September 2024

Composting of feedlot manure is a common practice in developed countries which aims at taking advantage of its value as reclamation and fertilizer as well as at reducing its negative impact on the environment. In Argentina manure is often applied without treatments of stabilization and hygienization. The aim of this work was to evaluate the effect of two rates of bulking agent (sawdust-woodshavings) and feedlot manure (1:1 and 2:1) on composting efficiency and final compost quality in experiments conducted as turning piles. Three piles per treatment were prepared at a pilot scale (3 m3each). The termophilic phase lasted 70-100 days and the content of faecal coliforms, potentially toxic materials and parameters of stability and maturity ? the latter being evaluated through water soluble C (WSC), ratio WSC/total N, ammonium, ratio ammonium/nitrate and germination index ? , indicated that after 4-6 months the product was innocuous, stable and mature. Pathogen and potentially toxic element contents were below the limits established by international laws. Compost 1:1 showed higher concentrations of total and extractable P than compost 2:1, and similar C and N values. When mixed with control soil both composts increased P and N availability and microbial respiration.
Acupuncture navigation method integrated with augmented reality
Authors: Chiou Shin-Yan , He Meng-Ru 
Published: September 2024

BACKGROUND: Acupuncture and moxibustion are effective in alleviating symptoms, but the large number of acupoints can make accurate needle placement and training difficult. OBJECTIVE: To address these challenges, this study aims to develop an augmented reality (AR) acupuncture navigation system designed to improve the accuracy and intuitiveness of acupoint localization. METHODS: The proposed system employs a six-point registration and positioning technique, enabling the AR navigation model to adapt to the specific characteristics of each patient. RESULTS: In testing, discrepancies between virtual and actual acupuncture points ranged from 0.6 mm to 3.9 mm, which is within the acceptable tolerance range for acupuncture. CONCLUSION: This AR-based system shows promise in enhancing the precision of acupuncture point localization, potentially leading to improved treatment outcomes.
The effects of mindfulness-focused internet-based cognitive behavioral therapy on elevated levels of stress and symptoms of exhaustion disorder: A randomized controlled trial
Authors: Vernmark Kristofer , Hursti Timo , Blom Victoria , Asplund Robert Persson , Nathanson Elise , Engelro Linda , Radvogin Ella , Andersson Gerhard 
Published: September 2024

Background
Internet-based Cognitive Behavior Therapy (ICBT) and mindfulness interventions are commonly used to treat elevated levels of stress. There are however few high-quality studies that examine ICBT with integrated mindfulness components for symptoms of stress and exhaustion, and the role of mindfulness exercises in digital treatment.

Method
The aim of the present study was to evaluate if a mindfulness-focused ICBT-program could reduce symptoms of stress and exhaustion, and increase quality of life, in a randomized controlled trial including 97 self-referred participants between 18 and 65 years who experienced elevated levels of stress.

Results
The intervention group had significantly reduced symptoms of stress and exhaustion, and increased quality of life, compared to the control group. Compared with the controls, participants in the intervention group showed a significant improvement with moderate to large effects on the primary outcome measure perceived stress (d = 0.79), and the secondary outcomes, exhaustion (d = 0.65), and quality of life (d = 0.40). Participants in the ICBT group also increased their level of mindfulness (d = 0.66) during the program. The amount of mindfulness training was significantly associated with an increased level of mindfulness, which in turn was significantly associated with reduced stress symptoms.

Conclusions
Mindfulness-focused ICBT can be an effective method to reduce stress-related mental health problems and the amount of mindfulness training seems to be of importance to increase the level of experienced mindfulness after treatment.
An automatic cough counting method and system construction for portable devices
Authors: Wang Yixuan , Yang Kehaoyu , Xu Shaofeng , Rui Shuwang , Xie Jiaxing , Wang Juncheng , Wang Xin 
Published: September 2024

IntroductionCough is a common symptom of respiratory diseases, and prolonged monitoring of cough can help assist doctors in making judgments about patients’ conditions, among which cough frequency is an indicator that characterizes the state of the patient’s lungs. Therefore, the aim of this paper is to design an automatic cough counting system to monitor the number of coughs per minute for a long period of time.MethodsIn this paper, a complete cough counting process is proposed, including denoising, segment extraction, eigenvalue calculation, recognition, and counting process; and a wearable automatic cough counting device containing acquisition and reception software. The design and construction of the algorithm is based on realistically captured cough-containing audio from 50 patients, combined with short-time features, and Meier cepstrum coefficients as features characterizing the cough.ResultsThe accuracy, sensitivity, specificity, and F1 score of the method were 93.24%, 97.58%, 86.97%, and 94.47%, respectively, with a Kappa value of 0.9209, an average counting error of 0.46 counts for a 60-s speech segment, and an average runtime of 2.80 ± 2.27 s.DiscussionThis method improves the double threshold method in terms of the threshold and eigenvalues of the cough segments’ sensitivity and has better performance in terms of accuracy, real-time performance, and computing speed, which can be applied to real-time cough counting and monitoring in small portable devices with limited computing power. The developed wearable portable automatic cough counting device and the accompanying host computer software application can realize the long-term monitoring of patients’ coughing condition.
Inorganic pyrophosphate plasma levels in patients with GGCX-associated PXE-like phenotypes
Authors: Li Qiaoli , Troutman Catherine , Peckiconis Mary , Wurst Tamara , Terry Sharon F. 
Published: September 2024

IntroductionPseudoxanthoma elasticum (PXE) is an autosomal recessive ectopic calcification disorder clinically affecting the skin, eyes, and vascular system. Most cases of PXE are caused by inactivating pathogenic variants in the ABCC6 gene encoding a hepatic transmembrane efflux transporter, which facilitates the extracellular release of ATP, the precursor of inorganic pyrophosphate (PPi), a potent endogenous inhibitor of calcification. Pathogenic variants in GGCX, encoding γ-glutamyl carboxylase required for activation of vitamin K-dependent coagulation factors as well as matrix Gla protein (MGP) and Gla-rich protein (GRP), two inhibitors of ectopic calcification, have also been reported to cause cutaneous changes like those seen in PXE. While ectopic calcification in ABCC6 deficiency has been associated with reduced plasma levels of PPi due to loss of ABCC6 transport activity in the liver, plasma PPi levels have not been reported in patients with GGCX-associated phenotypes.MethodsWe analyzed five patients from three unrelated families on their clinical, laboratory, and molecular findings who carry biallelic variants in GGCX and present with phenotypic characteristics associated with PXE. The variants were identified using a next-generation sequencing panel consisting of 29 genes associated with ectopic calcification.Results and conclusionThis study demonstrates that in addition to ABCC6, GGCX variants can cause the PXE phenotype, expanding PXE and perhaps other heritable ectopic calcification disorders’ clinical and genetic heterogeneity. The results also show that the plasma concentrations of PPi in these patients are not reduced compared to healthy control individuals, suggesting that plasma PPi does not govern ectopic calcification in GGCX deficiency.
The causal relationship between immune cell traits and schizophrenia: a Mendelian randomization analysis
Authors: Du Jianbin , Baranova Ancha , Zhang Guofu , Zhang Fuquan 
Published: September 2024

IntroductionThe complex and unresolved pathogenesis of schizophrenia has posed significant challenges to its diagnosis and treatment. While recent research has established a clear association between immune function and schizophrenia, the causal relationship between the two remains elusive.MethodsWe employed a bidirectional two-sample Mendelian randomization approach to investigate the causal relationship between schizophrenia and 731 immune cell traits by utilizing public GWAS data. We further validated the causal relationship between schizophrenia and six types of white cell measures.ResultsWe found the overall causal effects of schizophrenia on immune cell traits were significantly higher than the reverse ones (0.011 ± 0.049 vs 0.001 ± 0.016, p &lt; 0.001), implying that disease may lead to an increase in immune cells by itself. We also identified four immune cell traits that may increase the risk of schizophrenia: CD11c+ monocyte %monocyte (odds ratio (OR): 1.06, 95% confidence interval (CI): 1.03~1.09, FDR = 0.027), CD11c+ CD62L- monocyte %monocyte (OR:1.06, 95% CI: 1.03~1.09, FDR = 0.027), CD25 on IgD+ CD38- naive B cell (OR:1.03, 95% CI:1.01~1.06, FDR = 0.042), and CD86 on monocyte (OR = 1.04, 95% CI:1.01~1.06, FDR = 0.042). However, we did not detect any significant causal effects of schizophrenia on immune cell traits. Using the white blood cell traits data, we identified that schizophrenia increases the lymphocyte counts (OR:1.03, 95%CI: 1.01-1.04, FDR = 0.007), total white blood cell counts (OR:1.02, 95%CI: 1.01-1.04, FDR = 0.021) and monocyte counts (OR:1.02, 95%CI: 1.00-1.03, FDR = 0.034). The lymphocyte counts were nominally associated with the risk of schizophrenia (OR:1.08,95%CI:1.01-1.16, P=0.019).DiscussionOur study found that the causal relationship between schizophrenia and the immune system is complex, enhancing our understanding of the role of immune regulation in the development of this disorder. These findings offer new insights for exploring diagnostic and therapeutic options for schizophrenia.
Molecular landscape of kidney allograft tissues data integration portal (NephroDIP): a curated database to improve integration of high-throughput kidney transplant datasets
Authors: Boshart Alex , Petrovic Stefan , Abovsky Mark , Pastrello Chiara , Farkona Sofia , Manion Kieran , Neupane Slaghaniya , Allen Maya , Jurisica Igor , Konvalinka Ana 
Published: September 2024

IntroductionKidney transplantation is the optimal treatment for end-stage kidney disease; however, premature allograft loss remains a serious issue. While many high-throughput omics studies have analyzed patient allograft biospecimens, integration of these datasets is challenging, which represents a considerable barrier to advancing our understanding of the mechanisms of allograft loss.MethodsTo facilitate integration, we have created a curated database containing all open-access high-throughput datasets from human kidney transplant studies, termed NephroDIP (Nephrology Data Integration Portal). PubMed was searched for high-throughput transcriptomic, proteomic, single nucleotide variant, metabolomic, and epigenomic studies in kidney transplantation, which yielded 9,964 studies.ResultsFrom these, 134 studies with available data detailing 260 comparisons and 83,262 molecules were included in NephroDIP v1.0. To illustrate the capabilities of NephroDIP, we have used the database to identify common gene, protein, and microRNA networks that are disrupted in patients with chronic antibody-mediated rejection, the most important cause of late allograft loss. We have also explored the role of an immunomodulatory protein galectin-1 (LGALS1), along with its interactors and transcriptional regulators, in kidney allograft injury. We highlight the pathways enriched among LGALS1 interactors and transcriptional regulators in kidney fibrosis and during immunosuppression.DiscussionNephroDIP is an open access data portal that facilitates data visualization and will help provide new insights into existing kidney transplant data through integration of distinct studies and modules (https://ophid.utoronto.ca/NephroDIP).
Myasthenia Gravis: utilising cross-platform quantitative content analysis to uncover and validate unmet needs
Authors: Legg David , Meisel Andreas , Stein Maike , Gerischer Lea , Herdick Meret , Krüger Daniela , Mergenthaler Philipp , Masanneck Lars , Lehnerer Sophie 
Published: September 2024

BackgroundRecent years have seen a rapid growth in the number of online health communities targeted at patients with long-term conditions. Myasthenia Gravis (MG) is a rare neurological disease for which such communities have not been analysed before. The aim of this study was to better understand the needs of the MG population through the collation and categorisation of questions that users of MG social media were asking fellow users on these platforms.MethodologySystematic observation of four MG Facebook groups was conducted over a 2-month period. Groups were selected for analysis based on the following systematic criteria: Language (English), Membership (≥ 5,000 members), group activity (≥ 2 posts per week), target audience (general MG population) and researcher engagement with group administrators. The study protocol was reviewed by the institutional review board of the Charité—Universitätsmedizin Berlin (EA2/106/22). During the observation period, data were extracted from individual posts featuring questions made across each group using a systematic and objective coding scheme. All data points were coded directly from the source and collated into an SPSS database (IBM SPSS V.27, SPSS). Absolute and relative frequencies were calculated for categorical variables and proportions were compared across groups to validate the credibility and relevance of different requests.ResultsOf the 2,062 posts observed (N = 2,062), 1,392 featured questions (n = 1,392). Questions were asked by 787 unique users: 531 were identified as one-time users (67%) and 256 were identified as repeat users (33%). Six hundred and fifty six users were classified as presumed diagnosed (83%), 61 as seeking diagnosis (8%), 69 as family and/or friends (9%) and as other (&lt;0%). Eight unique categories of questions were observed including MG treatment (31%), Symptoms (19%), Living with MG (12%), Diagnosis (10%), non-MG medication (11%), Tests (8%), Location (4%) and Other (4%).ConclusionMembers of the MG population make active use of online health communities to seek and discuss practical information concerning various aspects of the disease, its diagnosis and care. The openness and willingness of the sample population to share sensitive medical information shows a high need for information not entirely catered to by the medical profession.
Unraveling the pathogenic mechanism of a novel filamin a frameshift variant in periventricular nodular heterotopia
Authors: Xue Chunran , Wang Yishu , Peng Jing , Feng Sisi , Guan Yangtai , Hao Yong 
Published: September 2024

BackgroundPeriventricular nodular heterotopia (PVNH) is a neuronal migration disorder caused by the inability of neurons to move to the cortex. Patients with PVNH often experience epilepsy due to ectopic neuronal discharges. Most cases of PVNH are associated with variations in filamin A (FLNA), which encodes an actin-binding protein. However, the underlying pathological mechanisms remain unclear.MethodsNext-generation sequencing was performed to detect variants in the patient with PVNH, and the findings were confirmed using Sanger sequencing. Iterative threading assembly refinement was used to predict the structures of the variant proteins, and the search tool for the retrieval of interacting genes/proteins database was used to determine the interactions between FLNA and motility-related proteins. An induced pluripotent stem cell (iPSC) line was generated as a disease model by reprogramming human peripheral blood mononuclear cells. The FLNA expression in iPSCs was assessed using western blot and quantitative real-time polymerase chain reaction (qRT-PCR). Immunofluorescence analysis was performed to determine the arrangement of F-actin.ResultsA novel FLNA frameshift variant (NM_001456.3: c.1466delG, p. G489Afs*9) was identified in a patient with PVNH and epilepsy. Bioinformatic analysis indicated that this variation was likely to impair FLNA function. Western blot and qRT-PCR analysis of iPSCs derived from the patient’s peripheral blood mononuclear cells revealed the absence of FLNA protein and mRNA. Immunofluorescence analysis suggested an irregular arrangement and disorganization of F-actin compared to that observed in healthy donors.ConclusionOur findings indicate that the frameshift variant of FLNA (NM_001456.3: c.1466delG, p. G489Afs*9) impairs the arrangement and organization of F-actin, potentially influencing cell migration and causing PVNH.
Weakly supervised localization model for plant disease based on Siamese networks
Authors: Chen Jiyang , Guo Jianwen , Zhang Hewei , Liang Zhixiang , Wang Shuai 
Published: September 2024

ProblemsPlant diseases significantly impact crop growth and yield. The variability and unpredictability of symptoms postinfection increase the complexity of image-based disease detection methods, leading to a higher false alarm rate.AimTo address this challenge, we have developed an efficient, weakly supervised agricultural disease localization model using Siamese neural networks.MethodsThis model innovatively employs a Siamese network structure with a weight-sharing mechanism to effectively capture the visual differences in plants affected by diseases. Combined with our proprietary Agricultural Disease Precise Localization Class Activation Mapping algorithm (ADPL-CAM), the model can accurately identify areas affected by diseases, achieving effective localization of plant diseases.Results and conclusionThe results showed that ADPL-CAM performed the best on all network architectures. On ResNet50, ADPL-CAM’s top-1 accuracy was 3.96% higher than GradCAM and 2.77% higher than SmoothCAM; the average Intersection over Union (IoU) is 27.09% higher than GradCAM and 19.63% higher than SmoothCAM. Under the SPDNet architecture, ADPL-CAM achieves a top-1 accuracy of 54.29% and an average IoU of 67.5%, outperforming other CAM methods in all metrics. It can accurately and promptly identify and locate diseased leaves in crops.
Development and validation of the Arabic children’s strong communication scale: a pilot study
Authors: Alhamami Munassir , Alduais Ahmed , Alasmari Muhammad , Qasem Fawaz 
Published: September 2024

BackgroundThe assessment of communication skills in Arabic-speaking children has been challenged by a lack of culturally and linguistically appropriate tools. The Arabic children’s strong communication scale (ACSCS) was developed in response to this need, adapting the children’s communication checklist-2 (CCC-2) to better suit the Arabic context.AimsThis study aimed to validate the ACSCS and establish its utility in measuring communication strengths among Arabic-speaking children.MethodsA total of 102 participants completed the questionnaire, which included relatives, teachers, and speech therapists of children aged 4–16. Twenty-two items were developed that targeted children communication strengths. The items were based on a theoretical framework that encompassed language proficiency, social communication, and behavioral aspects. Exploratory factor analysis (EFA) and confirmatory composite analysis (CCA) were employed to validate the structure of the ACSCS. The scale’s reliability was tested using Cronbach’s alpha and composite reliability, while its validity was assessed through convergent and discriminant validity measures.ResultsThe EFA and CCA confirmed a clear distinction of communication strengths. Reliability analyses indicated high internal consistency for the ACSCS. Discriminant validity was established, showing that the ACSCS accurately measures distinct facets of communication skills.ConclusionThe ACSCS is a valid and reliable instrument for assessing communication skills in Arabic-speaking children, reflecting strengths across various domains.ImplicationsThe scale has significant implications for educational and clinical settings, providing a culturally sensitive tool for practitioners and researchers to assess and support the communication development of Arabic-speaking children.
Embedding positive psychology into curriculum to promote posttraumatic growth, psychological flexibility, and socio-emotional competencies in higher education
Authors: Chim Kathleen , Lai JoJo T. C. , Chan Benjamin Tak Yuen 
Published: September 2024

BackgroundThe benefits of positive psychology interventions (PPIs) have become increasingly popular. While there is an emerging evidence base on the effectiveness of applying positive psychology in curriculum as preventive, early mental health intervention for higher education students, little is known about the content and pedagogy in these promising courses.ObjectiveThis article describes (a) the rationale for and development of a positive psychology course embedded into the curriculum that aims to foster posttraumatic growth, psychological flexibility, and socio-emotional competencies for higher education students; and (b) a mixed-method non-randomised pre-post study to evaluate the effectiveness of the positive psychology course in achieving positive participant outcomes.MethodsHigher education students from different disciplines will be enrolled to a general education course, “Positive Psychology and Personal Growth”, where they will learn progressive topics and complete summative assessments related to key areas in positive psychology. In addition to lectures, participants will engage in constructivist-based experiential activities that are guided by research on PPIs, life-design interventions and constructivist learning principles. The primary outcome is posttraumatic growth, and the secondary outcomes are psychological flexibility, and socio-emotional competencies.Expected resultsWe hypothesised that after the curriculum-embedded PPI, participants will have significantly higher levels of posttraumatic growth, psychological flexibility, and socio-emotional competencies. Results derived from the questionnaire survey will be supported by corroborating evidence identified from qualitative analysis of participants' summative assessments and follow-up semi-structured interviews on their perceptions of the present course.DiscussionThe current study will fill in a gap in existing intervention research and practise in curriculum-embedded PPIs and promote research transparency and pedagogical advancement. The intervention provides guidance and recommendations for educators to consider embedding positive psychology into the formal curriculum as cost-effective, low-intensity, structured, and sustainable educational interventions for higher education students.