bluuebunny/crossref_metadata_2025_split huggingface dataset:
USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51‐AKT signaling
Authors: Luo Kuntian , Li Yunhui , Yin Yujiao , Li Lei , Wu Chenming , Chen Yuping , Nowsheen Somaira , Hu Qi , Zhang Lizhi , Lou Zhenkun , Yuan Jian 
Published: April 2017

AbstractThe AKT pathway is a fundamental signaling pathway that mediates multiple cellular processes, such as cell proliferation and survival, angiogenesis, and glucose metabolism. We recently reported that the immunophilin FKBP51 is a scaffolding protein that can enhance PHLPP‐AKT interaction and facilitate PHLPP‐mediated dephosphorylation of AKT at Ser473, negatively regulating AKT activation. However, the regulation of FKBP51‐PHLPP‐AKT pathway remains unclear. Here we report that a deubiquitinase, USP49, is a new regulator of the AKT pathway. Mechanistically, USP49 deubiquitinates and stabilizes FKBP51, which in turn enhances PHLPP's capability to dephosphorylate AKT. Furthermore, USP49 inhibited pancreatic cancer cell proliferation and enhanced cellular response to gemcitabine in a FKBP51‐AKT‐dependent manner. Clinically, decreased expression of USP49 in patients with pancreatic cancer was associated with decreased FKBP51 expression and increased AKT phosphorylation. Overall, our findings establish USP49 as a novel regulator of AKT pathway with a critical role in tumorigenesis and chemo‐response in pancreatic cancer.
Rae1/YacP, a new endoribonuclease involved in ribosome‐dependent mRNA decay in Bacillus subtilis
Authors: Leroy Magali , Piton Jérémie , Gilet Laetitia , Pellegrini Olivier , Proux Caroline , Coppée Jean‐Yves , Figaro Sabine , Condon Ciarán 
Published: April 2017

AbstractThe PIN domain plays a central role in cellular RNA biology and is involved in processes as diverse as rRNA maturation, mRNA decay and telomerase function. Here, we solve the crystal structure of the Rae1 (YacP) protein of Bacillus subtilis, a founding member of the NYN (Nedd4‐BP1/YacP nuclease) subfamily of PIN domain proteins, and identify potential substrates in vivo. Unexpectedly, degradation of a characterised target mRNA was completely dependent on both its translation and reading frame. We provide evidence that Rae1 associates with the B. subtilis ribosome and cleaves between specific codons of this mRNA in vivo. Critically, we also demonstrate translation‐dependent Rae1 cleavage of this substrate in a purified translation assay in vitro. Multiple lines of evidence converge to suggest that Rae1 is an A‐site endoribonuclease. We present a docking model of Rae1 bound to the B. subtilis ribosomal A‐site that is consistent with this hypothesis and show that Rae1 cleaves optimally immediately upstream of a lysine codon (AAA or AAG) in vivo.
Cerebral salt wasting following traumatic brain injury
Authors: Taylor Peter , Dehbozorgi Sasan , Tabasum Arshiya , Scholz Anna , Bhatt Harsh , Stewart Philippa , Kumar Pranav , Draman Mohd S , Watt Alastair , Rees Aled , Hayhurst Caroline , Davies Stephen 
Published: April 2017

Summary
Hyponatraemia is the most commonly encountered electrolyte disturbance in neurological high dependency and intensive care units. Cerebral salt wasting (CSW) is the most elusive and challenging of the causes of hyponatraemia, and it is vital to distinguish it from the more familiar syndrome of inappropriate antidiuretic hormone (SIADH). Managing CSW requires correction of the intravascular volume depletion and hyponatraemia, as well as mitigation of on-going substantial sodium losses. Herein we describe a challenging case of CSW requiring large doses of hypertonic saline and the subsequent substantial benefit with the addition of fludrocortisone.

Learning points:

The diagnosis of CSW requires a high index of suspicion. Distinguishing it from SIADH is essential to enable prompt treatment in order to prevent severe hyponatraemia.

The hallmarks of substantial CSW are hyponatraemia, reduced volume status and inappropriately high renal sodium loss.

Substantial volumes of hypertonic saline may be required for a prolonged period of time to correct volume and sodium deficits.

Fludrocortisone has a role in the management of CSW. It likely reduces the doses of hypertonic saline required and can maintain serum sodium levels of hypertonic saline.
How Changes in Anti-SD Sequences Would Affect SD Sequences in Escherichia coli and Bacillus subtilis
Authors: Abolbaghaei Akram , Silke Jordan R , Xia Xuhua 
Published: April 2017

Abstract
The 3′ end of the small ribosomal RNAs (ssu rRNA) in bacteria is directly involved in the selection and binding of mRNA transcripts during translation initiation via well-documented interactions between a Shine-Dalgarno (SD) sequence located upstream of the initiation codon and an anti-SD (aSD) sequence at the 3′ end of the ssu rRNA. Consequently, the 3′ end of ssu rRNA (3′TAIL) is strongly conserved among bacterial species because a change in the region may impact the translation of many protein-coding genes. Escherichia coli and Bacillus subtilis differ in their 3′ ends of ssu rRNA, being GAUCACCUCCUUA3′ in E. coli and GAUCACCUCCUUUCU3′ or GAUCACCUCCUUUCUA3′ in B. subtilis. Such differences in 3′TAIL lead to species-specific SDs (designated SDEc for E. coli and SDBs for B. subtilis) that can form strong and well-positioned SD/aSD pairing in one species but not in the other. Selection mediated by the species-specific 3′TAIL is expected to favor SDBs against SDEc in B. subtilis, but favor SDEc against SDBs in E. coli. Among well-positioned SDs, SDEc is used more in E. coli than in B. subtilis, and SDBs more in B. subtilis than in E. coli. Highly expressed genes and genes of high translation efficiency tend to have longer SDs than lowly expressed genes and genes with low translation efficiency in both species, but more so in B. subtilis than in E. coli. Both species overuse SDs matching the bolded part of the 3′TAIL shown above. The 3′TAIL difference contributes to the host specificity of phages.
Genotyping-by-Sequencing Facilitates a High-Density Consensus Linkage Map forAegilops umbellulata, a Wild Relative of Cultivated Wheat
Authors: Edae Erena A , Olivera Pablo D , Jin Yue , Rouse Matthew N 
Published: April 2017

AbstractHigh-density genetic maps are useful to precisely localize QTL or genes that might be used to improve traits of nutritional and/or economical importance in crops. However, high-density genetic maps are lacking for most wild relatives of crop species, including wheat. Aegilops umbellulata is a wild relative of wheat known for its potential as a source of biotic and abiotic stress resistance genes. In this work, we have developed a framework consensus genetic map using two biparental populations derived from accessions PI 298905, PI 542369, PI 5422375, and PI 554395. The framework map comprised 3009 genotype-by-sequence SNPs with a total map size of 948.72 cM. On average, there were three SNPs per centimorgan for each chromosome. Chromosome 1U was the shortest (66.5 cM), with only 81 SNPs, whereas the remaining chromosomes had between 391 and 591 SNP markers. A total of 2395 unmapped SNPs were added to the linkage maps through a recombination frequency approach, and increased the number of SNPs placed on the consensus map to a total of 5404 markers. Segregation distortion was disproportionally high for chromosome 1U for both populations used to construct component linkage maps, and thus segregation distortion could be one of the probable reasons for the exceptionally reduced linkage size for chromosome 1U. From comparative analysis, Ae. umbellulata chromosomes except 4U showed moderate to strong collinearity with corresponding homeologous chromosomes of hexaploid wheat and barley. The present consensus map may serve as a reference map in QTL mapping and validation projects, and also in genome assembly to develop a reference genome sequence for Ae. umbellulata.
A Bayesian Poisson-lognormal Model for Count Data for Multiple-Trait Multiple-Environment Genomic-Enabled Prediction
Authors: Montesinos-López Osval A , Montesinos-López Abelardo , Crossa José , Toledo Fernando H , Montesinos-López José C , Singh Pawan , Juliana Philomin , Salinas-Ruiz Josafhat 
Published: April 2017

Abstract
When a plant scientist wishes to make genomic-enabled predictions of multiple traits measured in multiple individuals in multiple environments, the most common strategy for performing the analysis is to use a single trait at a time taking into account genotype × environment interaction (G × E), because there is a lack of comprehensive models that simultaneously take into account the correlated counting traits and G × E. For this reason, in this study we propose a multiple-trait and multiple-environment model for count data. The proposed model was developed under the Bayesian paradigm for which we developed a Markov Chain Monte Carlo (MCMC) with noninformative priors. This allows obtaining all required full conditional distributions of the parameters leading to an exact Gibbs sampler for the posterior distribution. Our model was tested with simulated data and a real data set. Results show that the proposed multi-trait, multi-environment model is an attractive alternative for modeling multiple count traits measured in multiple environments.
ĐẶC ĐIỂM SINH HỌC SINH SẢN CỦA NGÁN (AUSTRIELLA CORRUGATA) TẠI VÙNG TRIỀU VEN BIỂN TỈNH QUẢNG NINH
Authors: Thanh Nguyen Xuan , Nhan Dinh Van , Trang Tran Thi Thu , Long Luc Van , An Tran Viet , Hung Do Hong 
Published: April 2017

Kết quả phân tích 360 mẫu ngán (A. corrugata) thu tại các khu vực nơi có ngán phân bố thuộc vùng triều ven biển tỉnh Quảng Ninh từ tháng 1/2015 đến tháng 12/2015 cho thấy: Tuyến sinh dục ngán đực có màu trắng sữa, ngán cái có màu đen thẫm. Mùa vụ sinh sản của ngán hàng năm được xác định từ giữa tháng 4 đến đầu tháng 9, mùa sinh sản tập trung từ đầu tháng 6 đến đầu tháng 8. Ở các tháng tromg năm, tỷ lệ con đực luôn chiếm ưu thế so với con cái trong quần đàn ngán. Vào mùa sinh sản tỷ lệ đực cái cân bằng. Ngán lưỡng tính xuất hiện với tỷ lệ nhỏ (3,3 - 10%) vào mùa sinh sản và ở nhóm kích thước lớn hơn 40 mm. Ngán thành thục sinh dục lần đầu khi chiều dài đạt trên 30 mm, khối lượng trên 20 g. Sức sinh sản tuyệt đối giao động từ 340.690 - 2.090.000 trứng/cá thể, trung bình đạt 960.680 trứng/cá thể. Ấu trùng của ngán phát triển trải qua các giai đoạn chính: Ấu trùng chữ D, ấu trùng đỉnh vỏ và ấu trùng chân bò. Sau đó, ngán phát triển thành con non. Kết quả nghiên cứu góp phần cung cấp cơ sở khoa học cho việc xây dựng kế hoạch sản xuất giống nhân tạo, bảo vệ và phát triển bền vững nguồn lợi ngán tại địa phương.
Violence and injury observatories: Reducing the burden of injury in high-risk communities
Authors: Jabar Ardil , Matzopoulos Richard 
Published: April 2017

Background The meaning of an observatory has expanded recently from its origins in astronomy to that of specialised informational repositories and knowledge building centres housing cross-referenced databases with advanced analytic and research capacities. The purpose of the violence and injury observatories is directed toward preventing crime and violence at the local and regional level. Data around the burden of violence and injury for the African continent is limited and often not collected periodically thus limiting the implementation and evaluation of timely violence prevention interventions.MethodsAn observatory is primarily a tool to support the authorities in formulating effective responses to citizen safety and security issues. It is a centre dedicated to systematising information from different sources to produce periodic analyses or studies, to show the development of crime and violence in a given area. These surveillance systems primarily comprise two components: (1) Collection, analysis and interpretation of violence and injury data (2) Dissemination of findings to stakeholders within the safety and security cluster and the general public. There are several important data collection strategies which include health surveys, administrative data, mandatory reports and studies of special groups. Surveillance data initially may be analysed in terms of time, place, and person, by looking at time trends and geographic distribution and comparing age, sex, and population groups. Advanced data analysis methods for surveillance data include space-time clustering, time-series analysis, geospatial analysis, life tables, logistic regression, trend and small area analysis and methods for the forecast of epidemics based on surveillance dataConclusion Violence and injury observatories seeks to collect comprehensive data on violence and injury data with the system serving as a platform for violence prevention intervention development and evaluation for their respective sites.
A Comparative Study of the Strategies Employed in "The Old Man and the Sea" Translated from English into Persian on the Basis of Vinay and Darbelnet's Model
Authors: Sharei Vahideh 
Published: April 2017

Translation as a culture-based phenomenon has been investigated from various perspectives and it is a field of knowledge that is wide open and has not been exhausted yet. The aim of this study is to examine the use of Vinay and Darbelnet’s translation strategies in translation of the novel “The Old Man and the Sea” from English to Persian by Najaf Daryabandari and Mohammad Taghi Faramarzi. It also made an attempt to investigate which of the two main categories of translation strategies (direct and oblique translation) is the prevalent tendency in translation of this novel by the two translators.
Comparison between pre and post- operative chronic otitis media outcome (COMOT-15) in patients who underwent cartilage myringoplasty in Kathmandu University Hospital: The Nepal scenario
Authors: Shrestha Bikash Lal , Amatya Ram Chhaya Man , Dhakal Ashish , Pradhan Akash , Rajbhandari Pradip 
Published: April 2017

<p class="abstract"><strong>Background:</strong> The study was conducted to compare the pre and post-operative outcome of quality of life in patients who underwent cartilage myringoplasty with COMOT-15 (Chronic otitis media outcome test-15).</p><p class="abstract"><strong>Methods:</strong> 37 patients were included in the study that underwent endoscopic cartilage myringoplasty. COMOT-15 questionnaire was used pre and 6 months post-operatively to see the quality of life outcome. Along with that audiological testing was also performed. </p><p class="abstract"><strong>Results:</strong> There were total 37 patients (males 19 and females 18) with the mean age of 24.32±/-11.1years, and age range of 18-61years participated in all questionings and examination. The comparison of COMOT-15 Pre and Post-operative questionnaires analysis showed statistically significant improvement in all scales except for mental health. The audiological result also showed clear association of audiometry results with hearing function.</p><p><strong>Conclusions:</strong> The cartilage myringoplasty has beneficial effect in disease specific health related quality of life, as well there is clear association between hearing function scale and audiological evaluation. So, it is useful to perform cartilage myringoplasty wherever necessary.</p>
Validating internal electron and proton energy configurations via a theoretical derivation of the mass ratio mp/me
Authors: Oakley William S. 
Published: April 2017

<p><strong>Background:</strong> There are no particle models giving theoretical rest mass energy values for the electron or proton, and their internal energy configurations are unknown. Consequently there is no theoretical basis for the proton/electron rest mass ratio m<sub>p/</sub>m<sub>e</sub>. Previous articles established both electrons and protons consist of quantum loops of the same 6.8MeV base quantum energy, albeit in different relativistic states.</p><p><strong>Methods:</strong> Prior work is extended by considering internal particle energy cross coupling factors to derive detailed theoretical expressions for the internal energy distributions of electrons and protons. These expressions consist of the base quantum energy modified by terms containing only relativistic factors of the fine structure constant, α ~ 1/137. For m<sub>p</sub>/m<sub>e</sub> the base quantum energy cancels and the derived mass ratio is given by the particle’s internal quantum loop relativistic states. The derived mass ratio is compared to the empirical value. Newton’s gravitational constant, G, is calculated from the electron internal energy configuration.</p><p><strong>Results:</strong> Derived particle energy configurations give proton mass and proton/electron mass ratio values fully consistent with empirical data. The common base quantum loop energy is obtained to 6ppm. Combining particle mass energy expressions gives m<sub>p</sub>/m<sub>e</sub> to ten digits and consistent with the 2014 CODATA value via an expression containing only the fine structure constant. A theoretical value for Newton’s gravitational constant is obtained to an uncertainty of 6ppb. The Hierarchy problem is resolved, and the Planck scale of matter is adjusted. </p><p><strong>Conclusions:</strong> The particle energy configurations are validated by providing particle mass energy values and a proton/electron mass ratio consistent with empirical data. Newton’s G is shown not a natural constant, and misunderstanding its nature gave rise to the Hierarchy problem and an erroneous value for the Planck scale of matter, both now resolved.</p>
Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells
Authors: Sandra Ferry , Sudiono Janti , Trisfilha Pretty , Pratiwi Deviyanti 
Published: April 2017

BACKGROUND: Alpinia galanga (A. galanga) was reported as a potential medicinal source due to its wide effect. A. galanga rhizome crude extract (ARCE) was reported to have high cytotoxic effect in cancer cells, but low in normal cells. However half maximal inhibitory concentration (IC50) of ARCE is not clearly known yet. Hence, current study was conducted to investigate the IC50 of ARCE in normal standard fibroblast cell line, NIH-3T3 cells.METHODS: Rhizomes of A. galanga were collected, peeled, dried, milled and weighed. Extraction was performed using maceration method, then filtered and evaporated. ARCE with various concentrations were applied in NIH-3T3 cells for 24 or 48 hours. Cells were documented and counted with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Five hundreds grams of simplicia were macerated with ethanol and evaporated, 1 mg/mL crude extract with total volume of 114 mL was obtained. By addition of ARCE in NIH-3T3 cell culture, number of NIH-3T3 cells were shown less when treated with higher concentration of ARCE. Cell numbers of 0, 3.125, 6.25, 12.5, 25 and 50% ARCE treatment for 24 hours are 11,531, 11,352, 10,920, 10,365, 9,471, 8,360, respectively, meanwhile for 48 hours are 13,219, 12,686, 12,278, 11,390, 10,279, 8,390, respectively.CONCLUSION: IC50 of ARCE in 24 hours treatment was 620.5 mg/mL, while in 48 hours treatment was 666.6 mg/mL. Hence, ARCE is suggested to have low cytotoxic effect in NIH-3T3 cells.KEYWORDS: Alpinia galanga, ginger, extract, cytotoxic, MTT, NIH-3T3
Correlation Abnormal Ankle-Brachial Index and Multivessel Coronary Artery Disease in Acute Coronary Syndrome Patients
Authors: Mappangara Idar , Putra Magma Purnawan , Saleh Khalid 
Published: April 2017

BACKGROUND: Many studies showed the association between peripheral artery disease (PAD) and coronary artery disease (CAD). The anklebrachial index (ABI) was a simple, noninvasive, and not expensive test that showed high sensitivity and specificity in the diagnosis of PAD. Previous studies showed PAD correlation with the number of coronary artery lesions and higher complexity of the lesions. These correlations might contribute to worse cardiovascular outcomes, especially acute coronary syndrome (ACS) cases. Aim of this study is to evaluate the correlation abnormal ABI with the risk of ACS patients to have multivessel coronary artery lesions.METHODS: This was a retrospective analytical casec ontrol study. The data were taken from Dr. Wahidin Sudirohusodo Hospital medical records from November 2015 to February 2016. The inclusion criteria were (>18 years old) patients with ACS who had underwent ABI examination and coronary angiography.RESULTS: The prevalence of patients with ST-elevation myocardial infarction (STEMI) was lower than non-ST elevation ACS (NSTE-ACS). The prevalence of patients with abnormal ABI was 43.3% and patients with multivessel CAD was 65%. Male patients dominated the ACS population with 73.3%, about half of patients were smoking, and 86.7% had dyslipidemia. Patients with abnormal ABI and multivessel disease had greater number than patients with normal ABI (p=0.025). Analysis with binary logistic regression model showed abnormal ABI(odd ratio [OR] 4.83; p=0.021) and male sex (OR 19.35; p=0.010) were associated with greater risk of multivessel CAD.CONCLUSION: An abnormal ABI is associated with greater risk of multivessel CAD in ACS patients.KEYWORDS: ankle-brachial index, multivessel, coronary, acute coronary syndrome
Genome Editing with Crispr-Cas9 Systems: Basic Research and Clinical Applications
Authors: Meiliana Anna , Dewi Nurrani Mustika , Wijaya Andi 
Published: April 2017

BACKGROUND: Recently established genome editing technologies will open new avenues for biological research and development. Human genome editing is a powerful tool which offers great scientific and therapeutic potential.CONTENT: Genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein 9 (Cas9) technology is revolutionizing the gene function studies and possibly will give rise to an entirely new degree of therapeutics for a large range of diseases. Prompt advances in the CRISPR/Cas9 technology, as well as delivery modalities for gene therapy applications, are dismissing the barriers to the clinical translation of this technology. Many studies conducted showed promising results, but as current available technologies for evaluating off-target gene modification, several elements must be addressed to validate the safety of the CRISPR/Cas9 platform for clinical application, as the ethical implication as well.SUMMARY: The CRISPR/Cas9 system is a powerful genome editing technology with the potential to create a variety of novel therapeutics for a range of diseases, many of which are currently untreatable.KEYWORDS: genome editing, CRISPR-Cas, guideRNA, DSB, ZFNs, TALEN
Pressupostos teóricos e perspectivas didáticas na docência universitária
Authors: Consaltér Evandro 
Published: April 2017

Esta resenha abordará o livro “Docência universitária: pressupostos teóricos e perspectivas didáticas”, publicado pela Editora Mercado de Letras, em outubro de 2015. Além de participantes diretos do grupo, também se somam à obra outros colaboradores, de outras instituições nacionais e também internacionais. A organização da coletânea é de competência dos professores Altair Alberto Fávero, Carina Tonieto e Leandro Carlos Ody. A obra se originou a partir das investigações e discussões sobre essa temática, presentes no grupo de pesquisa Improvisação Docente no Contexto da Expansão da Educação Superior, com a coordenação do professor doutor Altair Alberto Fávero e desenvolvido junto ao programa de Mestrado e Doutorado em Educação e do Curso de Filosofia da Universidade de Passo Fundo.