bluuebunny/crossref_metadata_2025_split huggingface dataset:
ID: 1033 Case report: Results of the first case of Stem cell transplantation from cord blood cryopreservation for β-thalassemia patient at Vinmec International Hospital
Authors: NT Phu , Dam Phuong T.M. , NT Dung , PT Lan , NT Liem 
Published: September 2017

Background: To report the result of first case of β thalassemia, which is successfully transplanted by using allogeneic cord blood at the Vinmec International Hospital.
Methods: Male patient, born in January 2014, was diagnosed with β thalassemia at 3 months of age. Patient was received cord blood transplantation from his younger sibling who matches HLA index of 10/10 in August 2016.
Results: The number of cells in cord blood met high quality and after 30 days of transplantation, engraftment of patient fully developed with the Chimerism test was 100%. After 3 months of follow-up, it was demonstrated that the transplantation was successful.
Conclusion: We present an effective case of hematopoietic stem cell transplantation from human umbilical cord blood of sibling for treatment of thalassemia.
ID: 1041 Effectiveness of human adipose-derived stem cell therapy pretreated with hepatocyte growth factor in liver fibrosis mouse model
Authors: Vo Ngoc Hong , Le Trinh Van , Nguyen Nam Hai , Nguyen Yen K.T. , Dang Thanh Minh , Do Huy Quang 
Published: September 2017

Background: Adipose-derived stem cells (ADSCs) have the potential therapeutic impact on the liver fibrosis. Hepatocyte growth factor (HGF) is pivotal for damage repair with its anti-apoptotic, anti-fibrotic and cell migration-promoting effect. In this study, the therapy with HGF-pretreated hADSCs was expected to enhance the therapeutic effect in the amelioration of liver fibrosis compared with untreated hADSCs.

Method: HGF-hADSCs were prepared by culturing hADSCs for seven days in the medium added HGF.
HGF-hADSCs transplantation was performed to investigate the therapeutic effect of these cells on carbon tetrachloride (CCl4)-induced liver fibrosis in a mouse model.
Results: After seven days and fourteen days of cell transfusion, HGF-hADSCs ameliorated the liver fibrosis. The results showed the attenuation of the liver injury (ALT level), the down-regulation of procollagen-1 (7 days, 3-fold; 14 days, 6.7-fold) and alpha -smooth muscle actin (alpha-SMA) expression (7 days, 10-fold; 14 days, 2-fold), and the histological improvement. Notably, there was the significant difference in the procollagen-1 between HGF-hADSCs and untreated hADSCs groups. Thus, the therapy with HGF-hADSCs was more efficient in the liver fibrosis treatment compared with untreated hADSCs.
Conclusion: HGF pretreated hADSCs have a potential therapy in the treatment of liver fibrosis
ID: 1046 Antifungal prophylaxis in hematopoietic stem cell transplantation recipients: drug use evaluation at Vietnam National Institute of Hemotology and Blood transfusion
Authors: T. Son Nguyen , Nguyen Hoa M. , Nguyen Anh H. , Nguyen Tan D. , Vo Binh T.T. 
Published: September 2017

*For correspondence:
tungson94@gmail.com

Competing interests: The authors declare that no competing interests exist.
Received: 2017-07-13
Accepted: 2017-08-04
Published: 2017-09-05

Copyright The Author(s) 2017. This article is published with open access by BioMedPress (BMP).
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Background: Hematopoietic stem cell transplantation (HSCT) recipients were known to be in high risk of fungal infection. However, not all HSCT patients need antifungal prophylaxis. The aim of this study was to describe the characteristics of the use of antifungal prophylaxis in hematopoietic stem cell transplantation recipients and to evaluate the appropriateness of use.
Methods: In this observational prospective study, adult hematopoietic stem cell transplantation recipients without using antifungal treatment were recruited from start of stem cell transfusion in Vietnam National Institute of Hematology and Blood Transfusion (from April to December 2016) to end of antifungal prophylaxis or start of antifungal treatment. Appropriateness was defined based on National Comprehensive Cancer Network (NCCN) guidelines. Clinically significant Drug-drug interactions related to antifungals were defined based on Hansten and Horn’s Drug interaction and management 2013.
Results: 38 patients (mean age: 35.7 ± 12.0; 57.9% male) were admitted, included 21 autologous and 17 allogeneic HSCT recipients. After HSCT, 3 deaths, 1 to ICU, 1 IFI. Azoles prophylaxis was given to 100% of patients with median 17.5 days (13 - 47.5). Fluconazole was the most used antifungal agents, in 94.7% of patients and 74.5% of episodes. 10 patients had been changed antifungal agents. Indication was appropriate in 44.7%, 0% in autologous and 100% in allogeneic group. In patients with appropriate indication, choice of antifungal agents was 100% appropriate. 100% of patients used inappropriate dosage, mostly lower than recommeded dosage with azoles. 12.7% had appropriate prophylactic time. Potential drug-drug interactions were identified in 92.1% of patients. Most frequent interactions involved azoles-diazepam (80.4% of episodes) and azoles-immunosuppressive drugs (49% of episodes).
Conclusion: Our evaluation revealed a high proportion of inappropriate of antifungal prophylaxis. An antifungal stewardship programme is needed to strengthen rational use of antifungal in this specific circumstance.
ID: 1049 Variation of mitochondrial DNA HV1 and HV2 of the Vietnamese population
Authors: Quy Linh Nguyen , Pham Le Anh Tuan , Trinh Anh Hong , Nguyen Thuy Thi , Tran Thinh Huy , Tran Anh Van , Nguyen Hinh Duc , Ta Van Thanh , Tran Van Khanh 
Published: September 2017

The sequence polymorphism of mitochondrial DNA (mtDNA) hypervariable Segment 1 (HV1) and hypervariable Segment 2 (HV2) are studied and applied to genetic diversity and human evolution assessment, forensic genetics, consanguinity determination, and mitochondrial disease diagnosis.
Nucleotide sequence variations in HV1 and HV2, two hypervariable segments of the noncoding control region of human mitochondrial DNA (mtDNA), in selected ethnics of the Vietnamese population were elucidated through sequencing. In this study, we define the variations of HV1 and HV2 of 517 unrelated Vietnamese individuals in Kinh, Muong, Cham, and Khmer ethnic. We found 50 haplogroups: F1a haplogroup frequency is the highest at 15.7%; B5a haplogroup frequency is 10.8%, M haplogroup frequency is 8.9%, M7b1 haplogroup frequency is 7.7%; B6, D4e, D5a, E, F1c, F2a, F3a, G2a, M9b, N, N21 and U5a haplogroup frequencies are the lowest (1%). The frequency of SNP A263G are 100%; A73G is 99.6%, 315insC is 96%; 309insC is 56%; C16223T is 41%, and T16189C is 39%. We have assessed the genetic polymorphism of mtDNA HV1 and HV2 of 517 Kinh, Muong, Cham, Khmer ethnic samples.
ID: 1055 Adipose-derived stem cells and platelet rich plasma ameliorate liver cirrhotic circumstance in CCl4-induced mice: a new approach for liver cirrhosis treatment
Authors: Truong Nhung Hai , Nguyen Nam Hai , Le Trinh Van , Huynh Nghia , Ngo Dat Quoc , Nguyen Thanh Van , Phan Ngoc Kim , Pham Phuc Van 
Published: September 2017

Background: Stem cell therapy in liver cirrhosis treatment is attracting the attention of the scientific community. Adipose tissue-derived mesenchymal stem cells are a potential source of cells because they have self-renewal, high proliferation, and differentiation into a variety of cell types, including hepatocytes as potential cell sources for cirrhosis treatment. Platelet-rich plasma (PRP) growth factors contribute to regeneration and wound healing. We test the hypothesis that PRP co-administration enhances MSC treatment for mouse cirrhosis.
Method: Male Swiss mice were treated orally with olive oil or CCl4 for 11 weeks. PRP was obtained from healthy mice. Mouse adipose-derived stem cells (mADSCs) from adipose tissue of 3 weeks CCl4 mice were cultured for three passages (P3-mADSCs) before the transfer by tail vein injection with or without PRP into 11 weeks CCl4 mice. Mice were divided into six groups (n=10 each group). 1) normal, 2) cirrhotic, 3) cirrhotic /PBS; 4) cirrhotic/PRP (0.2 ml/mice with PRP from healthy mice), 5) cirrhotic/mADSCs (5 x 105 cells/mice), and 6) cirrhotic/mADSC-PRP.

Result: mADSCs were highly positive for CD44, CD90, and CD105. Relative to liver cells, P3-mADSCs highly expressed Alb, Ck18, Ck19, Tnf, c-met, Cyp1a1, Afp, Muc1, Ldl receptor. mADSCs were strongly positive for Cyp1a1 (98.21±1.57%) and Hgf (95.55±3.11%); moderately positive for alfa-fetoprotein (45.99±2.08%), Aat (44.43±7.79%), Alb (57.81±8.49%) and differentiated into hepatocyte-like cells under induction medium. After transplantation, CFDA-transplanted cells into CCl4-treated mice were found in the liver at day 21 st. Compared to mADSCs, mADSCs and PRP co-treatment most effectively improved serum AST/ALT/bilirubin and albumin levels in day seven cirrhotic mice (p<0.05); and significantly down-regulated procollagen (104-fold less) and TGF-beta 1 (10-fold less) in day 21 cirrhotic liver. Histology index and collagen deposition were improved in 100% of mADSC/PRP- and mADSCs- cirrhotic mice compared to 33.3% of PBS- or PRP- cirrhotic liver (p<0.05).
Conclusion: Cultured mADSCs express hepatocyte enriched markers. PRP coadministration enhances mADSC effects to improve liver function further, and further reduce fibrosis.
ID1056 Synthesis and biological evaluation of novel curcumin derivative with water-soluble phosphate group as potential antitumor agent
Authors: Nguyen Dinh Luyen , Nguyen Van Giang , Nong Thi Bich Van , Pham Thi Hien , Nguyen Van Hai 
Published: September 2017

Background: Curcumin (diferuloylmethane) is the main natural active polyphenol from the rhizome of Curcuma longa (turmeric) and in others Curcuma spp. The main pharmacological effects include anti-tumor, anti-inflammatory, anti-oxidation, anti-fungal and anti-bacterial activities. However, the clinical application of curcumin is limited by its main drawbacks such as low solubility, poor bioavailability and rapid excretion from the body. In this study, the chemical modification approach was performed to improve the solubility of curcumin in water, at the same time increase the anti-tumor activity.
Methods: The water-soluble compound (2-(curcumin-O-yl)ethyl dihydrophosphate, LH-42017) was synthesized by two classical reactions: O-alkylation with 2-bromoethanol to obtain mono-O-(2-hydroxyethyl)-curcumin, followed by esterification using phosphoryl chloride in water and pyridine. The chemical structure was confirmed by IR-, NMR-spectroscopies and mass-spectrometry. The anti-tumor activity of LH-42017 was assessed against hepatocellular carcinoma Hep-G2, human breast cancer MCF-7, human leukemia K562 and human cervical carcinoma HeLa cell lines based on the sulforhodamine B (SRB) assay.
Results: LH-42017 was successfully synthesized from curcumin by overall yield of 28.43%. IC50 of original curcumin was 63.55, 90.88, 104.99 and 60.24 µM on Hep-G2, MCF-7, K562 and Hela, respectively. IC50 of LH-42017 was 36.82, 51.60, 60.80 and 26.14 µM on Hep-G2, MCF-7, K562 and Hela, respectively.
Conclusion: The novel synthesized compound LH-42017 showed the antitumor activity in 1.7-2.3 times better than original curcumin. The water-soluble phosphate group plays an important role in development of natural curcumin as potential antitumor agent.
ID: 1060 Organic-inorganic Biomaterials promote mesenchymal stem cell proliferation and differentiation for long-term cultivation
Authors: Yusoff Umul Hanim , Ebrahimi Shamsi 
Published: September 2017

Background: A nifty propagating of mesenchymal stem cell (MSCs) diligence has germinated all over the world by innovative investigators. However, the clinical and basic research applications of MSC requires novel finding biomaterials interfacial interaction especially in sustainable the morphology, physiology, multipotent and phenotypically in long-term cultivation. A prominent of biomaterials benefit to MSCs culture has triggered the multitudinous field especially in regenerative medicine. In order to hinder the deprivation of MSCs in purity and potency, the alternative cell-substrate materials of MSCs culture is essentially to be discovered.
This has instigated the idea to encountered the method of screening libraries organic and inorganics biomaterials in bio-adhesively, free ethically, and sustainability to support the morphologically, physiologically, multi-potent and phenotypically of substrates coating cover slip.
Methods: Libraries of inorganic biomaterials substrates have been collected from co-researcher to conduct the initial screening phase of 100 myriad fabrications of substrates whereas enumerated as a Graphene Oxide (GO), Hydroxyapatite (HAp), and Bioactive Glass (BAG) coated cover slip and discs also several organic biomaterials. Wharton’s Jelly derived Mesenchymal Stem Cells (WJMSCs) and Denuded Amnion Mesenchymal Stem Cells (AMMSCs) have been seeded on each substrate in the 48-well plate. Top four leading substrates have been selected for further cultivation until up to 5 passage (>P5) for long term screening known as scaling up phase. Several parameters such as cell attachment, cell viability, kinetic growth, cell-materials osteogenic and adipogenic differentiation and cell phenotype have been analyzed. Top one cell-material culture will go forward to further long-term cultivation up to Passage 10(>P10).
Results: Morphologically and phenotypically demonstrated that GOy1WJMSC showed the significance result among others.
ID:1073 Assessment of intravenous infused human adipose derived stem cells homing in diabetic mice
Authors: Truong Nhat Chau , Vu Ngoc Bich , Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Bui Anh Thi Van , Vu Anh Thi Van , Dang Loan Thi Tung , Phan Ngoc Kim 
Published: September 2017

Currently, Adipose-derived Stem Cells (hASCs) therapy is being promoted applied research in the treatment of many diseases, especially autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis and type 1 diabetes. However, profound mechanisms of this potential therapy have not been understood entirely. It is questioned that which tissues transplanted cells home to, and how long they survive there?
In this work, we evaluated the migration, and survival duration of GFP-expressed hASCs (hASC-GFPs) in normal and STZ-induced diabetic mice. A number of 106 hASC-GFPs were intravenous transplanted into the mice. After 1, 3, 5, 7 days, the presence of hASC-GFPs in various organs such as heart, lungs, kidneys, spleen, liver and pancreas was detected by Imaging technique (iBox® ExplorerTM Imaging Microscope System_IMS) and the percentage of these cells in the tissues of diabetic mice was evaluated by Flow cytometry (FCM).
The results showed that the transplanted cells could be identified by both IMS and FCM. By IMS, it was clear that hASC-GFPs homed to lung and heart most at day 1 and 3, respectively and the signals were decreased gradually in both tissues. These imaging results were similar in normal and diabetic mice. By FCM, we detected the signals of hASC-GFPs in all six diabetic organs at day 1, 3, 5, 7. The signals expressed highest at day 1 (liver, heart), day 3 (lungs, kidneys, spleen) or day 5 (pancreas) and all gradually decreased until day 7.
Our results demonstrated that although difficult hASCs could home to the pancreas of normal as well as diabetic mice, but we need more experiments to make further conclusions regards the mechanism of their immigration and behaviour in the recipients.
ID: 1081 Development and validation of surface electrocardiogram in the septal accessory pathway localization in typical Wolff-Parkinson-White syndrome
Authors: Chu Si Dung , Pham Khanh Quoc , Tran Dong Van 
Published: September 2017

Objectives: This study was designed characteristics of surface electrocardiogram (ECG) for the localization of septal accessory pathway (AP) in the typical Wolff-Parkinson-White (WPW) syndrome to develop a new algorithm ECG for the septal AP localization, and to test the accuracy of the algorithm prospectively.
Subject and Methods: We studied 106 patients, in 65 patients with typical WPW syndrome have a single anterograde with the localization of Aps identified by successful radiofrequency catheter ablation (RCFA) to develop a new ECG algorithm for the septal AP localization. Then this algorithm was tested propectively in 41 patients were compared with the location of AP’s successful ablation by RCFA (gold standard).
Results: We found that the 12 lead ECG parameters in 65 patients with typical WPW syndrome such as the transition of the QRS complex, delta wave polarity in V1 lead, delta wave polarity in at least 2/3 inferior leads and morphology QRS was “QRS pattern’’ in inferior leads in diagnosis for the localization of septal APs with hight accuracy predicted from 83.3% to 100%, and for development of a new ECG algorithm. Then the following 41 patients were prospectively evaluated by the new derived algorithm for the septal pathways with high sensitivity and specificity from 84.6% to 100%.
Conclusion: 12-lead ECG parameters in typical WPW syndrome closely related to the septal AP localization, in order to develop the new ECG algorithm by parameters as above; and can be used to a new septal ECG algorithm in predicted the location APs with high accuracy predicted
Seroprevalence of Human Herpesvirus Type 2 in a Reference Center for Pregnant Women in Rio de Janeiro, Brazil
Authors: Lima Lyana , Padalecki Guilherme , Castro Carlos , Cordeiro Jânio , De Paula Vanessa 
Published: September 2017

Introduction: Pregnant women stand as an relevant group for research about Human Herpesvirus (HHV-2) infection owing to the risk of mother-to-child transmission. Methods: Women attending in a prenatal care center were tested for HHV-2 IgM and IgG by ELISA. Quantitative PCR test was the chosen method to ascertain viremia. Results: The seroprevalence of IgG and IgM anti-HHV-2 was 20.6% and 2.2% respectively. HHV-2 viremia was found in one pregnant woman with HHV-2 IgM, leading to the assumption of primary infection. Conclusion: The significantly high prevalence of HHV-2 found and the ascertainment of primary infection in a pregnant woman underline the need for constant HHV-2 follow-up and diagnosis in order to avoid sexual transmission.
Бунин как «буревестник» новой революции
Authors: Ильинский Игорь Михайлович 
Published: September 2017

<p>Статья дает авторское осмысление роли творчества И. А. Бунина и его произведений предреволюционного периода в формировании образа России и русского человека в сознании читающей публики. В 1910 г. Бунин опубликовал свое главное произведение — «Деревня». В нем писатель изобразил целую галерею деревенских персонажей, наделенных чертами якобы «типичного русского характера»: отрицательными, а часто совершенно отрицательными. Сама жизнь в ситуации постоянного подавления со стороны «хозяев» (дворян и помещиков), бытовое неустройство, голод, болезни — все это создает безнадежный образ «мужика», лишенного каких-либо добродетелей, и тяжелейшей порочности обитателей деревенского мира. Раскрывая в «Деревне» особенности труднейших и суровых условий жизни сельского населения в 1904–1907 гг., Бунин, хочется думать, сам того не осознавая, вызывал в умах современников понимание того, что единственный путь для исправления мрачной картины бытия 85% русского народа — радикальные перемены и смена правящего строя. </p>
Проблемы социализации личности студента в вузе
Authors: Ковалева Антонина Ивановна 
Published: September 2017

<p>В данной статье на основе авторской концепции социализации рассматриваются проблемы личностного становления студентов на этапе обучения в вузе, характеризуются специфика современной высшей школы как института социализации, особенности социализационных траекторий российских студентов, значимые для студентов личностные качества и их оценки учебно-воспитательного процесса. В статье использованы результаты последнего этапа (весна 2017 г.) мониторингового исследования проблем студенческой молодежи, проведенного в Московском гуманитарном университете. </p><p>Показано, что в оценках студентов МосГУ преобладает высокий уровень удовлетворенности обучающихся условиями обучения в университете. Подтверждается ранее установленная в мониторинге тенденция: студенты, удовлетворенные наличием учебной и методической литературы, оснащением учебных аудиторий, уровнем информационных технологий, выше оценивают и свою удовлетворенность вузом в целом. Студенты, не принимающие участия в общественной жизни университета, ниже оценивают уровень своей удовлетворенности вузом, нежели студенты, участвующие во внеучебных мероприятиях. Однако сохраняется проблема вовлечения студентов в такие мероприятия, в работу органов студенческого самоуправления, инициативные студенческие проекты, поддерживаемые университетом. Остается доля студентов (около 11%), которые отмечают слабые стороны организации учебного процесса.</p><p>Полученные в исследовании данные свидетельствуют о сложных процессах в формировании мировоззрения, нравственных позиций, социальной активности студентов МосГУ.</p>
Самореализация личности по возрастным интервалам (на примере современных россиян)
Authors: Козлова Татьяна Захаровна 
Published: September 2017

<p class="Text">Самореализация человека, — главная цель всей его жизни. Жизненный путь человека — непрерывная цепь этапов самореализации. Самореализация личности — это актуализации индивида в двух основных сферах жизни: профессии (труде) и семье человека. Объект исследования — возрастные группы граждан России (в возрасте от 18 лет и старше): 1) «18–30лет»; 2) «31–40 лет»; 3) «41–50 лет»; 4) «51–60 лет»; 5) «старше 60 лет». Возраст является важной характеристикой личности. Он — индикатор биологического и психологического статуса человека, а значит, в определенной степени его социальной активности. С возрастом происходит снижение умственных способностей и сужение сферы мотивации. Этот тезис подтвержден в исследованиях западных ученых (Р. Кюлен, Б. Ньюгартен и др.). Предмет исследования — самореализация личности. Сферы профессиональной деятельности являются теми институтами, где реализуется личность. Ответ на вопрос, каков уровень самореализации лиц различных возрастных групп, автор статьи получила, анализируя репрезентативное Всероссийское социологическое исследование, которое провел Институт социологии РАН в конце 2016 г. Выборка — 4000 человек. Респондентам был задан вопрос: «Оцените по 5-балльной шкале, насколько важным лично для Вас на сегодняшний день является: самореализация, проявление себя». На шкале от 1 до 5 определены значения: «не важно» — для оценки 1; «важно» — для оценки 4; «очень важно» — для оценки 5. Самореализацию мы определяем суммой двух оценок 4 и 5, Каков уровень самореализации личности по возрастным группам? У респондентов самой молодой возрастной группы «18–30 лет» сильная мотивация и умственные способности на пике развития. У них высокая самореализация — 87%. Второй интервал взрослой жизни — человек от 31 года до 40 лет, период наиболее активной реализации поставленных целей. У них также высокая самореализация — 83%. Третий возрастной период жизни человека — возраст после 50 лет. Человек стремится свои знания передать молодежи. Самореализация в этой возрастной группе — 74%. Предпенсионный период («51–60 лет»). Уход на пенсию сопряжен для многих людей с определенными переживаниями. Для возрастной группы «51–60лет» самореализация — 73%. В возрастной группе «60 и старше» у людей подтачиваются силы, самореализация понижается до 56%.</p>