bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversible Reduction of Estrone to 17β‐Estradiol by Rhizobium, Sphingopyxis, and Pseudomonas Isolates from the Las Vegas Wash
Authors: Blunt Susanna M. , Benotti Mark J. , Rosen Michael R. , Hedlund Brian P. , Moser Duane P. 
Published: March 2017

Environmental endocrine‐disrupting compounds (EDCs) are a growing concern as studies reveal their persistence and detrimental effects on wildlife. Microorganisms are known to affect the transformation of steroid EDCs; however, the diversity of estrogen‐degrading microorganisms and the range of transformations they mediate remain relatively little studied. In mesocosms, low concentrations of added estrone (E1) and 17β‐estradiol (E2) were removed by indigenous microorganisms from Las Vegas Wash water within 2 wk. Three bacterial isolates, Rhizobium sp. strain LVW‐9, Sphingopyxis sp. strain LVW‐12, and Pseudomonas sp. strain LVW‐PC, were enriched from Las Vegas Wash water on E1 and E2 and used for EDC transformation studies. In the presence of alternative carbon sources, LVW‐9 and LVW‐12 catalyzed near‐stoichiometric reduction of E1 to E2 but subsequently reoxidized E2 back to E1; whereas LVW‐PC minimally reduced E1 to E2 but effectively oxidized E2 to E1 after a 20‐d lag. In the absence of alternative carbon sources, LVW‐12 and LVW‐PC oxidized E2 to E1. This report documents the rapid and sometimes reversible microbial transformation of E1 and E2 and the slow degradation of 17α‐ethinylestradiol in urban stream water and extends the list of known estrogen‐transforming bacteria to the genera Rhizobium and Sphingopyxis. These results suggest that discharge of steroid estrogens via wastewater could be reduced through tighter control of redox conditions and may assist in future risk assessments detailing the environmental fate of estrogens through evidence that microbial estrogen transformations may be affected by environmental conditions or growth status.Core Ideas
Estrone and 17β‐estradiol were rapidly degraded in Las Vegas Wash water.
Microbial genera involved included Rhizobium, Sphingopyxis, and Pseudomonas.
Microbial isolates catalyzed the reversible reduction of estrone to 17β‐estradiol.
Oxidation‐reduction activities on estrogens may be controlled by redox status.
Assessing the Performance of Floating Biofilters for Oxidation of Methane from Dairy Effluent Ponds
Authors: Syed Rashad , Saggar Surinder , Tate Kevin , Rehm Bernd H.A. , Berben Peter 
Published: March 2017

Mitigating methane (CH4) emissions from New Zealand dairy effluent ponds using volcanic pumice soil biofilters has been found to be a promising technology. Because the soil column biofilter prototype previously used was cumbersome, here we assess the effectiveness of volcanic pumice soil‐perlite biofilter media in a floating system to remove high concentrations of CH4 emitted from a dairy effluent pond and simultaneously in a laboratory setting. We measured the CH4 removal over a period of 11 mo and determined methanotroph population dynamics using molecular techniques to understand the role of methanotroph population abundance and diversity in CH4 removal. Irrespective of the season, the pond‐floating biofilters removed 66.7 ± 5.7% CH4 throughout the study period and removed up to 101.5 g CH4 m−3 h−1. By contrast, the laboratory‐based floating biofilters experienced more biological disturbances, with both low (∼34%) and high (∼99%) CH4 removal phases during the study period and an average of 58% of the CH4 oxidized. These disturbances could be attributed to the measured lower abundance of type II methanotroph population compared with the pond biofilters. Despite the acidity of the pond biofilters increasing significantly by the end of the study period, the biofilter encouraged the growth of both type I (Methylobacter and Methylomonas) and type II (Methylosinus and Methylocystis) methanotrophs. This study demonstrated the potential of the floating biofilters to mitigate dairy effluent ponds emissions efficiently and indicated methanotroph abundance as a key factor controlling CH4 oxidation in the biofilter.Core Ideas
Floating biofilters removed an average of 67% of pond CH4 from a dairy effluent pond.
Floating biofilters removed up to 101.5 g CH4 m−3 h−1.
Diverse and abundant methanotroph community supported CH4 removal under varying conditions.
Study demonstrated potential of the biofilters to mitigate effluent pond emissions.
Metals and Metalloids in Wild Asparagus at Uncontaminated and Mining‐Contaminated Sites
Authors: Biddau R. , Cidu R. 
Published: March 2017

Wild edible plants are often considered healthier than crops because they are unaffected by fertilizers, herbicides, and pesticides. However, the occurrence of potentially toxic elements in wild plants is poorly known and needs to be assessed. In this study, 22 elements were determined by inductively coupled plasma mass spectrometry in wild asparagus (Asparagus acutifolius L.). Asparagus shoots were collected in uncontaminated environments (20 sites) and in areas contaminated by past mining (15 sites) in Sardinia, Italy. Beryllium, Bi, Te, Tl, and U were not detected in asparagus (<0.03 μg g−1); Ag, As, Co, Cd, Li, and Sb were detected at very low levels (median, 0.01–0.05 μg g−1); B, Cu, Fe, Mn, Rb, and Zn were relatively abundant (median, 10–58 μg g−1); and Ba, Mo, Ni, Pb, and Sr showed large variations (median, 0.4, 0.3, 3, 0.1, and 0.5 μg g−1, respectively). Median concentrations of detected elements were similar in asparagus collected in uncontaminated environments or previously mined areas. An exception was Cd in asparagus collected in mined areas (median, 1.4 μg g−1), whose levels were much higher than in asparagus from uncontaminated environments (median, 0.01 μg g−1), indicating that high Cd in bedrocks affect contents in asparagus shoots. Also, Sb‐ and As‐rich substrates in mined areas resulted in asparagus with Sb and As concentrations much higher than asparagus samples from areas mined predominantly for Pb and Zn. Results of this study indicate potential health hazards to residents living worldwide in areas affected by past mining.Core Ideas
Median amounts of elements in asparagus are similar in uncontaminated and mine areas, except Cd.
High As, Cd, and Sb levels in asparagus correspond to high content in soils.
Soil properties affect the translocation of Cd from soil to asparagus shoots.
Cd showed the highest value of estimated daily intake versus the guideline.
A Simple Method for Determining the Critical Point of the Soil Water Retention Curve
Authors: Chen Chong , Hu Kelin , Ren Tusheng , Liang Yin , Arthur Emmanuel 
Published: March 2017

Core Ideas
A fixed tangent line method was developed to estimate the critical point of the soil water retention curve as an alternative to the commonly used flexible tangent line method.
The critical points of the soil water retention curve from the proposed method and the flexible tangent line method were compared.
Particle‐size distribution and specific surface area had significant effects on the critical point of the soil water retention curve.
The transition point between capillary water and adsorbed water, which is the critical point Pc [defined by the critical matric potential (ψc) and the critical water content (θc)] of the soil water retention curve (SWRC), demarcates the energy and water content region where flow is dominated by capillarity or liquid film flow. Accurate estimation of Pc is crucial for modeling water movement in the vadose zone. By modeling the dry‐end (matric potential < –104.2 cm H2O) and wet‐end (matric potential > –104.2 cm H2O) sections of the SWRC using the models of Campbell and Shiozawa, and of van Genuchten, a fixed tangent line method was developed to estimate Pc as an alternative to the commonly used flexible tangent line method. The relationships between Pc, and particle‐size distribution and specific surface area (SSA) were analyzed. For 27 soils with various textures, the mean RMSE of water content from the fixed tangent line method was 0.007 g g–1, which was slightly better than that of the flexible tangent line method. With increasing clay content or SSA, ψc was more negative initially but became less negative at clay contents above ∼30%. Increasing the silt contents resulted in more negative ψc values, whereas soils with higher sand content had less negative ψc values. The magnitude of θc increased linearly with SSA and clay content. Our findings suggest that Pc estimated from the fixed tangent line method could represent the actual values and clarify the effect of particle‐size distribution and SSA on Pc.
Position of Shear Plane at the Clay–Water Interface: Strong Polarization Effects of Counterions
Authors: Liu Xinmin , Ding Wuquan , Tian Rui , Du Wei , Li Hang 
Published: March 2017

Core Ideas
A nonlinear Poisson–Boltzmann equation including ionic polarization effects was developed.
A new approach was developed to estimate the shear plane position at solid–liquid interface.
The strong polarization is an important factor in the change of shear plane position.
The zeta potential at the shear plane is an important electrochemical parameter at solid–liquid interfaces. However, it is difficult to accurately determine the position of the shear plane because a rather important effect— strong ion polarization in the electrical double layer—is neglected. In the present study, a model for estimation of the shear plane position considering ion polarization was developed based on the modified Poisson–Boltzmann equation, and the intensity of polarization on a constantly charged surface was quantified through an ion exchange equilibrium experiment. The polarization effects increased the effective ionic charges of Na+ from 1 to 1.082 and that of K+ from 1 to 1.498 at the montmorillonite–water interface, which resulted in decreasing the thickness of the slipping layer. The theoretical prediction of the swelling pressure was in excellent agreement with the experimental measurements. The theoretical standpoint also sheds new light on one of the oldest puzzles in physical chemistry—specific ion effects.
Hacia el surgimiento de una cultura de la innovación en un centro público de investigación en México: Hallazgos y aprendizajes
Authors: López Hernández Carlos Gerardo , Serrano-Santoyo Arturo 
Published: March 2017

El presente trabajo describe la trayectoria y las estrategias establecidas durante el periodo de 1973 a 2015 sobre el entorno de la cultura de la innovación, la propiedad intelectual y el desarrollo de conocimiento aplicado a la industria, gobierno y sociedad en un Centro Público de Investigación (CPI), el Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Se hace un recuento de elementos sustantivos de aprendizaje, hallazgos e hitos; a partir de una visión y estrategias de planeación alineado la política nacional de ciencia y tecnología, sus cambios en el tiempo y el impacto de la normatividad aplicada. Se discuten los factores que afectaron las iniciativas de cambio en los procesos hacia una vinculación exitosa y desarrollo tecnológico. Se presentan así mismo los aprendizajes, hallazgos y conclusiones, en donde un cambio de paradigma afectó la dinámica institucional de la innovación, lo que obligó a hacer una revisión de los objetivos institucionales para dar un viraje hacia una sólida cultura de la innovación y su reflejo en la sociedad.
Research Advances in Molecular Modeling in Cyclodextrins
Authors: Zhao Qianqian , Zhang Weixiang , Wang Runmiao , Wang Yitao , Ouyang Defang 
Published: March 2017

Background:
Cyclodextrins (CDs), as one type of the novel pharmaceutical excipients, have been
widely used in drug delivery and pharmaceutical industry. Over the past decades, a large amount of molecular
modeling studies in CDs were reported for profound understanding of structural, dynamic and energetic features
of CDs systems. Thus, this review is focused on qualitative and quantitative analysis of research outputs on molecular
modeling in CDs.

Methods:
The original data were collected from Web of Science and analyzed by scientific knowledge mapping
tools, including Citespace, Science of Science, VOSviewer, GPSvisualizer and Gephi software. Scientific knowledge
mapping, as an emerging approach for literature analysis, was employed to identify the knowledge structure
and capture the development of the science in a visual way.

Results:
The results of analysis included research outputs landscape, collaboration patterns, knowledge structure
and research frontiers shift with time. China had the largest contributions to the publication number in this area,
while USA dominated the high quality research outputs. International collaboration between USA and Europe
was much stronger than that within Asia. J American Chemical Society, as one of the most important journals,
played a pivotal role in linking different research fields. Furthermore, seven important thematic clusters were
identified by the research cluster analysis with visualization tools and demonstrated from three different perspectives
including: (1) the mostly-used CD molecules: β-Cyclodextrin, (2) preferred modeling tools: docking calculation
and molecular dynamic, (3) hot research fields: structural properties, solubility, chiral recognition and solidstate
inclusion complexes. Moreover, research frontier shift in the past three decades was traced by detecting
keywords bursts with high citation.

Conclusion:
The current review provided us a macro-perspective and intellectual landscape to molecular modeling
in CDs.
OPTIMIZATION OF SCREEN PRINTED REFERENCE ELECTRODE BASED ON CHARGE BALANCE AND POLY (BUTYL ACRYLATE) PHOTOCURABLE MEBRANE
Authors: Alva S. , Heng L. Y. , Ahmad M. 
Published: March 2017

<p class="TRANSAffiliation"><span>This research focus on transforming the traditional design of reference electrode into all-solid-state reference electrode front-end using Ag/AgCl screen- printed electrodes. By replacing the internal reference solution of a traditional reference electrode by a solid photocurable membrane, an all-solid-state reference electrode can be achieved. The solid-state screen-printed reference electrode was designed using a photocurable acrylic film containing immobilized sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (NaTFPB) and trimethylocthylammonium chloride (TOMA-Cl). An optimum ratio of NaTFPB:TOMA-Cl = 1:1 produced a stable reference electrode. In the anions interference studies, all anions i.e. NO<sub>3</sub><sup>-</sup>, Cl<sup>-</sup>, Br<sup>-</sup> and SO<sub>4</sub><sup>2-</sup> does not give effect to the SPRE except perchlorate anions. The all-solid-state reference electrodes was applied to the detection of potassium ions and ammonium ions. Validation of the all-screen-printed reference electrode was performed with reference electrode standard gel type. The validation results showed that all-solid-state screen-printed reference electrode demonstrated performance that was comparable to standard reference electrode. </span></p>
Hyper-intensive farming white shrimp Litopenaeus vannamei (Decapoda: Penaeidae) in a seawater tank under semi-controlled conditions
Authors: Ortega-Salas A. A. , Rendón M. L. A. 
Published: March 2017

Shrimp development to a commercial size in high density culture saves food and avoids predators and disease. Our study was conducted to calculate the growth of white shrimp Litopenaeus vannamei by hyper-intensive cultivation under semi-controlled conditions. We seeded at a density of 550 shrimp per m3 during the first cycle and 400 shrimp per m3 in the second cycle in an outdoor tank of 6m3or 6m2 covered with mesh, constant aeration. The shrimp were fed Artemia franciscana during the first two weeks and camaronina pellets (35% protein) as required, in food baskets, aftterwards. The temperature ranged from 22,3 to 31,3°C, pH 7,5-8,7, oxygen 4,26±1,43. The tanks are siphoned of debris every other day, and water was replaced according to a program. The food conversion ratio (FCR) was 1:1,3. The shrimp were measured weekly to calculate growth with the Bertalanffy model. Survival in the first cycle was 95,8 ( 97,9% for the second cycle). Population parameters (maximum likelihood method) for the first cycle were k=0,0301, L∞ =322,16 and t0 =-0,8852; second cycle: k=0,0203, L∞ =294,42 and t0 =-5,3771. There was rapid growth during the first 10 weeks. Biomass was 27kg for the first cycle (second: 16kg). KEY WORDSGrowth, high density, survival, biomass, semi-controlled conditions.
Neonate survival en handled broods of the Olive Ridley Turtle Lepidochelys olivacea (Testudines: Cheloniidae) in “El Verde”, Sinaloa, Mexico
Authors: Rendón Luis A. , Ortega-Salas Armando A. 
Published: March 2017

We worked in the protective and conservation area of the Olive Ridley Turtle Lepidochelys olivacea in the beaches of Estero Verde, Sinaloa, Mexico (2004 season). The position in the beach does not affect survival. We found nests until the preferential nesting site located at the beginning of the second sandy berm in a zone free of vegetation; and the latter presented a slight increase in survival. Specimens handled for 2-5 hours survived less (R2=0,82). KEY WORDSIncidence of turtles, management, survival, protected area, conservation.
Grupo focal sobre el desarrollo de productos innovadores a base de frijol gandul (Cajanus cajan) en la Cuenca del Río Peñas Blancas de Costa Rica
Authors: Andrés Carmen 
Published: March 2017

El gandul (Cajanus Cajan) es un producto conocido por sus propiedades como fijador de nitrógeno, alto valor nutricional y protector de cuencas hídricas. Su cultivo está extendido en América Latina, África y Asia, y se exporta a Estados Unidos y Europa. Se han desarrollado diferentes productos a partir del gandul, primordialmente enlatados. En Costa Rica existen plantaciones, pero no hay estudios científicos sobre este producto o sus subproductos. Es por eso que se decidió desarrollar un grupo focal que generara ideas innovadoras sobre los productos más factibles a industrializar a base de gandul. Entre estas ideas se destacaron: el gandul tierno enlatado, gandul seco, gandul tierno congelado, flanes y sopas deshidratadas, salsa tipo Chuncky, salsas para carnes, dips, bocadillos, tortillas y harina. PALABRAS CLAVEGandul, Cajanus cajan, grupo focal, innovación, productos nuevos.
Fatal predation of oilfish, Ruvettus pretiosus (Gempylidae), by a young short-finned pilot whale, Globicephala macrorhynchus (Delphinidae)
Authors: García-Rivas María del Carmen , Schmitter-Soto Juan J. , Pérez-Flores Jonathan 
Published: March 2017

We found a dead whale (Globicephalus macrorhynchus) whose probable cause of death was an internal injury produced by a 1m-long oilfish (Ruvettus pretiosus) that had bitten the whale´s stomach from the inside. This is the first record of an oilfish in Mexican Caribbean waters. KEY WORDSMarine mammals, mesopelagic fishes, feeding behavior, new records, Caribbean sea
Sex reversal, growth, and survival in the guppy Poecilia reticulata (Cyprinodontiformes: Poeciliidae) under laboratory conditions
Authors: Ortega-Salas A.A. , Reyes-Bustamante H. , Reyes B. H. 
Published: March 2017

Just like it is important to obtain robust and heavy fish in short periods in aquaculture, in aquariology it is important to efficiently obtain colorful fish, which are usually males, and this makes sex reversal important. We studied sex reversal and growth for 120 days in 10-gallon tanks, without aeration, with densities of 1 fry per liter. Temperature varied between 28,2 and 28,7°C. The pH was 8,1 to 8,8 and oxygen concentration 6,2 to 6,8mg/l. Aba Api-balanced food with 25% protein was used and three different hormones were added to the treatments: 1) Sten 2) Primoteston and 3) Sostenon 250. Sex reversal in males was 85,0%, 92,5% and 80,0% respectively. Fishes with treatments 1 and 3 gained an average of 0,48g and 4,2cm (with treatment 2: 0,44g and 4cm). Survival was 85%, 92,5% and 80%; however, these differences were not significant. The three treatments gave the expected results of sex reversal, growth and survival.KEY WORDSAnimal feed, population density, hormone supply, aquariums without aeration
Asthma knowledge among primary and secondary school teachers in rural northern Costa Rica
Authors: Pitstick Claire 
Published: March 2017

Asthma is a chronic inflammatory disease of the airways. It is very common among children and is a major cause for emergency room visits and missed school days. Costa Rica has one of the highestrates of childhood asthma in the world; so it is important for teachers to know how to prevent and treat asthma attacks in the classroom. Since schools in rural Costa Rica had not yet been studied, teachers were sampled from six schools in Venecia and Aguas Zarcas, San Carlos. During March and April 2014, 185 asthma questionnaires were collected.Among these teachers, 51% were aware of asthmatic students in their classes, and 19% recalled witnessing an asthma attack in the classroom. Interest in asthma training was high among 85% of the teachers,and the preferred format was a lecture or workshop. Greater asthma knowledge was significantly related to living with an asthmatic person (p=0,014), but not with being asthmatic, having asthmatic students,witnessing an asthma attack in the classroom, sex, age, education level, or teaching experience. Greater interest in asthma training was related to awareness of asthmatic students (p=0,031), prior experience with an asthma attack in the classroom (p=0,033), and greater asthma knowledge (p=0,022). Primary school teachers were more likely to be aware of asthmatic students in their classes compared to secondary school teachers (p=0,002)
Length-weight relationships of the Indian Major Carp, Labeo rohita (Cypriniformes: Cyprinidae) from six drainages of Ganga basin, India
Authors: Iqbal Mir Javaid , Kumar Sarkar Uttam , Prakash Gusain Om , Kumar Dwivedi Arvind 
Published: March 2017

The Indian Major Carp, Labeo rohita, is a geographically widespread and economically important food fish species in tropical freshwater of India and adjacent countries. We studied the lengthweightrelationships of 1 033 specimens collected from the main channel of Ganga river and its five major drainages from March 2009 to July 2012. The length of males ranged from 16 to 92cm (females: 16to 94cm). The growth is allometric positive (b>3) for males, females and pooled sexes. The coefficient of determination (r2) in males ranged from 0,978 to 0,989 and for females from 0,958 to 0,985. Data from field populations are scarce and our results will be useful in the management and conservation of L. rohita populations in its natural range.
Size, sex and maturity of the squid Lolliguncula diomedeae (Cephalopoda: Loliginidae) in the Gulf of Tehuantepec, México.
Authors: Alejo-Plata María del Carmen , Gómez-Márquez José Luis , Herrera-Galindo Jorge Eduardo 
Published: March 2017

There is practically no information on the biology of Lolliguncula (Loliolopsis) diomedeae. We analyzed specimens caught in four shrimp fishing trips in the Gulf of Tehuantepec in 1999-2001, 2004 and 2006.Sexual maturity and gonadosomatic index (GSI) were used as indicators of reproductive activity. A total of 1 200 individuals were captured, 89% female. Females ranged from 35 to 97mm in mantle length (LDM),with a mode at 80mm and weights 2 to 30,9g; males 20,7 to 65mm LDM with a mode at 35-40mm and weights from 0,4 to 12g. Differences in the LDM and the PT between males and females were significant (KS, p<0,05). The sex ratio was 8:1 H:M (p <0,05). The weight-length relationship type is potential for both sexes, with a negative allometric growth.83% of mature females had high IGS values, in contrast to less than 20% of mature males. The size at first maturity (L50) indicates that males (42,7mm LDM) mature when shorter than females (74,5mm LDM). Maturity, IGS and oceanographic features of the Gulf of Tehuantepec indicate that L. diomedeae spawn there.