bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteochondral Allograft Transplantation of the Knee in Patients Aged 40 Years and Older
Authors: Wang Dean , Kalia Vivek , Eliasberg Claire D. , Wang Tim , Coxe Francesca R. , Pais Mollyann D. , Rodeo Scott A. , Williams Riley J. 
Published: November 2017

Background: Treatment of large chondral defects of the knee among patients aged ≥40 years remains a difficult clinical challenge owing to preexisting joint degeneration and the lack of treatment options short of arthroplasty. Purpose: To characterize the survivorship, predictors of failure, and clinical outcomes of osteochondral allograft transplantation (OCA) of the knee among patients aged ≥40 years. Study Design: Case series; Level of evidence, 4. Methods: Prospectively collected data were reviewed for 54 consecutive patients aged ≥40 years who were treated with OCA. Preoperative levels of osteoarthritis (according to Kellgren-Lawrence classification) and meniscal volume and quality were graded from review of radiographs and magnetic resonance imaging. Complications, reoperations, and patient responses to validated outcome measures were reviewed. A minimum follow-up of 2 years was required for analysis. Failure was defined by any removal or revision of the allograft or conversion to arthroplasty. Results: Among 51 patients (mean age, 48 years; range, 40-63 years; 65% male), a total of 52 knees had symptomatic focal cartilage lesions (up to 2 affected areas) that were classified as Outerbridge grade 4 at the time of OCA and did not involve substantial bone loss requiring shell allografts or additional bone grafting. Mean duration of follow-up was 3.6 years (range, 2-11 years). After OCA, 21 knees (40%) underwent reoperation, including 14 failures (27%) consisting of revision OCA (n = 1), unicompartmental knee arthroplasty (n = 5), and total knee arthroplasty (n = 8). Mean time to failure was 33 months, and 2- and 4-year survivorship rates were 88% and 73%, respectively. Male sex (hazard ratio = 4.18, 95% CI = 1.12-27.13) and a higher number of previous ipsilateral knee operations (hazard ratio = 1.70 per increase in 1 surgical procedure, 95% CI = 1.03-2.83) were predictors of failure. A higher Kellgren-Lawrence osteoarthritis grade on preoperative radiographs was associated with higher failure rates in the Kaplan-Meier analysis but not the multivariate model. At final follow-up, clinically significant improvements were noted in the pain (mean score, 47.8 to 67.6) and physical functioning (56.8 to 79.1) subscales of the Short Form-36, as well as the International Knee Documentation Committee subjective form (45.0 to 63.6), Knee Outcome Survey–Activities of Daily Living (64.5 to 80.1), and overall condition statement (4.5 to 6.8) ( P < .001). No significant changes were noted for the Marx Activity Rating Scale (5.1 to 3.9, P = .789). Conclusion: A higher failure rate was found in this series of patients aged ≥40 years who were treated with OCA as compared with other studies of younger populations. However, for select older patients, OCA can be a good midterm treatment option for cartilage defects of the knee.
Solution-Focused Brief Therapy for Individuals With Alcohol Use Disorders in Chile
Authors: González Suitt Karla , Geraldo Pablo , Estay Marlene , Franklin Cynthia 
Published: November 2017

Purpose: This article presents a pilot study exploring the applicability of a linguistically adapted, solution-focused brief therapy (SFBT) program, implemented by social workers in Chilean primary care. Method: We completed a single-case design with eight replications. To analyze the results of the program on participants’ alcohol use and other related variables, we conducted visual and percentage of nonoverlapping data analyses. Results: Social workers successfully implemented 10 of the 13 SFBT techniques. Although results need to be interpreted with caution, positive trends were observed. Participants increased their “percentage of days abstinent,” diminished “consequences of alcohol use,” decreased their “depression index,” and increased their “self-reported well-being.” Discussion: Results are consistent with previous studies on SFBT and alcohol use. Exception and coping questions may serve to increase abstinent days. SFBT focus on issues other than alcohol that are important to clients could help to reduce harm on individuals who use alcohol.
Mass spectrometric characterization of protein structures and protein complexes in condensed and gas phase
Authors: Yefremova Yelena , Danquah Bright D , Opuni Kwabena FM , El-Kased Reham , Koy Cornelia , Glocker Michael O 
Published: November 2017

Proteins are essential for almost all physiological processes of life. They serve a myriad of functions which are as varied as their unique amino acid sequences and their corresponding three-dimensional structures. To fulfill their tasks, most proteins depend on stable physical associations, in the form of protein complexes that evolved between themselves and other proteins. In solution (condensed phase), proteins and/or protein complexes are in constant energy exchange with the surrounding solvent. Albeit methods to describe in-solution thermodynamic properties of proteins and of protein complexes are well established and broadly applied, they do not provide a broad enough access to life-science experimentalists to study all their proteins' properties at leisure. This leaves great desire to add novel methods to the analytical biochemist's toolbox. The development of electrospray ionization created the opportunity to characterize protein higher order structures and protein complexes rather elegantly by simultaneously lessening the need of sophisticated sample preparation steps. Electrospray mass spectrometry enabled us to translate proteins and protein complexes very efficiently into the gas phase under mild conditions, retaining both, intact protein complexes, and gross protein structures upon phase transition. Moreover, in the environment of the mass spectrometer (gas phase, in vacuo), analyte molecules are free of interactions with surrounding solvent molecules and, therefore, the energy of inter- and intramolecular forces can be studied independently from interference of the solvating environment. Provided that gas phase methods can give information which is relevant for understanding in-solution processes, gas phase protein structure studies and/or investigations on the characterization of protein complexes has rapidly gained more and more attention from the bioanalytical scientific community. Recent reports have shown that electrospray mass spectrometry provides direct access to six prime protein complex properties: stabilities, compositions, binding surfaces (epitopes), disassembly processes, stoichiometries, and thermodynamic parameters.
Hyperglycaemia and the SOAR stroke score in predicting mortality
Authors: McCall Stephen J , Alanazi Turkiah A , Clark Allan B , Musgrave Stanley D , Bettencourt-Silva Joao H , Bachmann Max O , Metcalf Anthony K , Bowles Kristian M , Mamas Mamas A , Potter John F , Myint Phyo K 
Published: November 2017

Background: We assessed the association between admission blood glucose levels and acute stroke mortality and examined whether there was any incremental value of adding glucose status to the validated acute stroke mortality predictor – the SOAR (stroke subtype, Oxford Community Stroke Project classification, age, and pre-stroke modified Rankin) score. Methods: Data from Norfolk and Norwich University Hospital stroke and Transient Ischaemic Attack register (2003–2013) and Anglia Stroke Clinical Network Evaluation Study (2009–2012) were analysed. Multivariable logistic regression analysis assessed the association between admission blood glucose levels with inpatient and 7-day mortality. The prognostic ability of the SOAR score was then compared with the SOAR with glucose score. Results: A total of 5575 acute stroke patients (ischaemic stroke: 89.2%) with mean age (standard deviation) of 76.97 ( ± 11.88 ) years were included. Both borderline hyperglycaemia (7.9–11.0 mmol/L) and hyperglycaemia (>11.0 mmol/L) when compared to normoglycaemia (4.0–7.8 mmol/L) were associated with both 7-day and inpatient mortality after controlling for sex, age, Oxford Community Stroke Project classification and pre-stroke modified Rankin score. Both the SOAR stroke score and SOAR-G score were good predictors of inpatient stroke mortality [area under the curve: 0.82 (95% confidence interval: 0.81–0.84) and 0.83 (95% confidence interval: 0.81–0.84)], respectively. These scores were also good at predicting outcomes in both patients with and without diabetes. Conclusion: High blood glucose levels at admission were associated with worse acute stroke mortality outcomes. The constituents of the SOAR stroke score were good at predicting mortality after stroke.
Evaluation for shunted pouches of cavernous sinus dural arteriovenous fistula and the treatment outcome of transvenous embolization
Authors: Sato Masaki , Izumi Takashi , Matsubara Noriaki , Nishihori Masahiro , Miyachi Shigeru , Wakabayashi Toshihiko 
Published: November 2017

Background This study aimed to evaluate the detailed location and the number (single or multiple) of cavernous sinus dural arteriovenous fistula (CSDAVF) shunted pouches as well as the relationship between the characteristics of shunted pouch(es) and the treatment outcome of transvenous embolization for CSDAVF. Methods A total of 23 consecutive patients with CSDAVFs who underwent angiogram and transvenous embolization were retrospectively analyzed. Shunted pouches were assessed using three-dimensional angiogram and multiplanar reformatted image obtained from the rotational angiogram data. Results Of the 23 patients with CSDAVFs, 40 shunted pouches were identified. Twelve CSDAVFs had a single shunted pouch, and 11 had multiple shunted pouches. The mean CSDAVF with multiple shunted pouches was 2.5. The shunted pouches were more often found in the posterior compartment of the CS, which was connected with the intercavernous sinus (23/40; 57.5%). In 12 CSDAVFs with a single shunted pouch, 10 were treated with selective embolization and complete occlusion was achieved during the follow-up. Two CSDAVFs with single shunted pouch were just observed without intervention, and DAVFs disappeared spontaneously during the follow-up period. In 11 CSDAVFs with multiple shunted pouches, eight were treated with selective embolization and three with sinus embolization. In six of eight (75%), complete occlusion was achieved following selective embolization, but two of eight (25%) recurred and required retreatment. Conclusions Rotational angiography data suggested that the shunted pouches of CSDAVFs were mostly located in the posterior compartment of the CS connected with the intercavernous sinus. Selective embolization for CSDAVFs with a single shunted pouch is the first-line treatment alternative to sinus packing, and selective embolization with multiple shunted pouches will be a considerable treatment option.
Early social communicative skills as predictors of symptom severity in autism spectrum disorder
Authors: Lobban-Shymko Jan , Im-Bolter Nancie , Freeman Nancy 
Published: November 2017

Background and aims Early diagnosis of autism spectrum disorder, while providing many benefits, also presents challenges. Accurately predicting symptom severity allows clinicians to confidently diagnose and assign the most appropriate intervention. Little available research predicts symptom severity in children with autism spectrum disorder who have not been exposed to significant levels of intervention. Methods The present file review study examined preverbal skills as predictors of symptom severity, approximately one year later, in a group of young children (18–64 months) with autism spectrum disorder ( n = 199). Results Of the two core diagnostic features (social communicative deficits and restricted repetitive behaviors), social communicative skills best predicted symptom severity. Furthermore, social communicative gestures predicted symptom severity after age, adaptive behavior, restricted repetitive behaviors, and functional gestures had been accounted for. Conclusions Social communicative gestures are excellent predictors of future symptom severity independent of intervention effects in very young children with autism spectrum disorder. Implications Previously, the social aspect of gestures has been missing in the literature on language and symptom prediction in children with autism spectrum disorder. Careful attention to social communicative gestures in the future may help with early diagnosis and more accurate predictions of symptom and developmental trajectories.
A novel irreversible FLT3 inhibitor, FF-10101, shows excellent efficacy against AML cells with FLT3 mutations
Authors: Yamaura Takeshi , Nakatani Toshiyuki , Uda Ken , Ogura Hayato , Shin Wigyon , Kurokawa Naoya , Saito Koichi , Fujikawa Norie , Date Tomomi , Takasaki Masaru , Terada Daisuke , Hirai Atsushi , Akashi Akimi , Chen Fangli , Adachi Yoshiya , Ishikawa Yuichi , Hayakawa Fumihiko , Hagiwara Shinji , Naoe Tomoki , Kiyoi Hitoshi 
Published: November 2017

Key Points
FF-10101 has selective and potent inhibitory activities against FLT3 by forming a covalent bond to the C695 residue. FF-10101 shows high efficacy against AML cells with FLT3 mutations including quizartinib-resistant activation loop mutations.
Platelet glycoprotein VI aids in local immunity during pneumonia-derived sepsis caused by gram-negative bacteria
Authors: Claushuis Theodora A. M. , de Vos Alex F. , Nieswandt Bernard , Boon Louis , Roelofs Joris J. T. H. , de Boer Onno J. , van ’t Veer Cornelis , van der Poll Tom 
Published: November 2017

Key Points
GPVI aids in local immunity in gram-negative pneumonia–derived sepsis. GPVI, CLEC2, and neutrophils do not play a major role in vascular integrity during pneumosepsis.
Mutant JAK3 signaling is increased by loss of wild-type JAK3 or by acquisition of secondary JAK3 mutations in T-ALL
Authors: Degryse Sandrine , Bornschein Simon , de Bock Charles E. , Leroy Emilie , Vanden Bempt Marlies , Demeyer Sofie , Jacobs Kris , Geerdens Ellen , Gielen Olga , Soulier Jean , Harrison Christine J. , Constantinescu Stefan N. , Cools Jan 
Published: November 2017

Key Points
One-third of T-ALL cases with JAK3 mutation harbor 2 JAK3 mutations. Double JAK3 mutants show stronger signaling than single JAK3 mutants.
Role of blue dye for sentinel lymph node detection in early endometrial cancer
Authors: Restaino Stefano , Ronsini Carlo , Finelli Angelo , Perrone Emanuele , Scambia Giovanni , Fanfani Francesco 
Published: November 2017

Abstract
Background
Sentinel Lymphonode analysis has become a barely new and innovative way to treat early stages of endometrial cancer (Ballester et al., Lancet Oncol 469–476, 2011; Buda et al., Ann Surg Oncol 2975-81, 2016). Indocyanine green cervical injection is considered gold standard for mapping nodes’ drainage. Blue dye is used as a valid alternative in many centers, due to the lower cost of execution. The objective of this video is to prove that methylene blue dye’s cervical injection is a valid and “low-cost” method to obtain mapping of lymphatic drainage in patient with early endometrial cancer.

Methods
Fifty-four-year old women, with a recent diagnosis of endometrial cancer IA G2, we performed a radical Hysterectomy type A. We diluted in equal proportions the blue dye and saline and injected 1 cl in depth and 1 cl on the surface of the cervix, at 3 o’clock and 9 o’clock. After 20 min, it was shown with precision the lymphatic drainage until the first lymph node station from both sides.

Results
One external iliac lymph node and one obturator were resected on the left side and one external iliac on the right side. On histological analysis, none of the lymph nodes had any location of metastasis, neither micro-metastasis. Myometrial infiltration was 8/19 mm.

Conclusions
Blue dye cervical injection is a “low-cost”, safe, and satisfactory procedure to point out Sentinel Lymph Node of uterus drainage. Other tracers, such as indocyanine green, are widely used in gynecological oncology, but with a higher cost of the product and the needing of a dedicated optical filter to be shown on human view.
Yeast Hog1 proteins are sequestered in stress granules during high-temperature stress
Authors: Shiraishi Kosuke , Hioki Takahiro , Habata Akari , Yurimoto Hiroya , Sakai Yasuyoshi 
Published: November 2017

ABSTRACT
The yeast high-osmolarity glycerol (HOG) pathway plays a central role in stress responses. It is activated by various stresses, including hyperosmotic stress, oxidative stress, high-temperature stress and exposure to arsenite. Hog1, the crucial MAP kinase of the pathway, localizes to the nucleus in response to high osmotic concentrations, i.e. high osmolarity; but, otherwise, little is known about its intracellular dynamics and regulation. By using the methylotrophic yeast Candida boidinii, we found that CbHog1-Venus formed intracellular dot structures after high-temperature stress in a reversible manner. Microscopic observation revealed that CbHog1-mCherry colocalized with CbPab1-Venus, a marker protein of stress granules. Hog1 homologs in Pichia pastoris and Schizosaccharomyces pombe also exhibited similar dot formation under high-temperature stress, whereas Saccharomyces cerevisiae Hog1 (ScHog1)-GFP did not. Analysis of CbHog1-Venus in C. boidinii revealed that a β-sheet structure in the N-terminal region was necessary and sufficient for its localization to stress granules. Physiological studies revealed that sequestration of activated Hog1 proteins in stress granules was responsible for downregulation of Hog1 activity under high-temperature stress.
This article has an associated First Person interview with the first author of the paper.
Learning to starve: impacts of food limitation beyond the stress period
Authors: McCue Marshall D. , Terblanche John S. , Benoit Joshua B. 
Published: November 2017

ABSTRACT
Starvation is common among wild animal populations, and many individuals experience repeated bouts of starvation over the course of their lives. Although much information has been gained through laboratory studies of acute starvation, little is known about how starvation affects an animal once food is again available (i.e. during the refeeding and recovery phases). Many animals exhibit a curious phenomenon – some seem to ‘get better’ at starving following exposure to one or more starvation events – by this we mean that they exhibit potentially adaptive responses, including reduced rates of mass loss, reduced metabolic rates, and lower costs of digestion. During subsequent refeedings they may also exhibit improved digestive efficiency and more rapid mass gain. Importantly, these responses can last until the next starvation bout or even be inherited and expressed in the subsequent generation. Currently, however, little is known about the molecular regulation and physiological mechanisms underlying these changes. Here, we identify areas of research that can fill in the most pressing knowledge gaps. In particular, we highlight how recently refined techniques (e.g. stable isotope tracers, quantitative magnetic resonance and thermal measurement) as well as next-generation sequencing approaches (e.g. RNA-seq, proteomics and holobiome sequencing) can address specific starvation-focused questions. We also describe outstanding unknowns ripe for future research regarding the timing and severity of starvation, and concerning the persistence of these responses and their interactions with other ecological stressors.
Considerations on the role of olfactory input in avian navigation
Authors: Wiltschko Roswitha , Wiltschko Wolfgang 
Published: November 2017

ABSTRACT
A large amount of data documents an important role of olfactory input in pigeon navigation, but the nature of this role is not entirely clear. The olfactory navigation hypothesis assumes that odors are carrying essential navigational information, yet some recent experiments support an activating role of odors. This led to an ongoing controversy. An important, often-neglected aspect of the findings on olfaction is that olfactory deprivation affects avian navigation only at unfamiliar sites. The orientation of anosmic birds at familiar sites remains an enigma; earlier assumptions that they would rely on familiar landmarks have been disproven by the home-oriented behavior of anosmic pigeons additionally deprived of object vision, which clearly indicated the use by the birds of non-visual, non-olfactory cues. However, if odors activate the establishing and enlarging of the navigational ‘map’ and promote the integration of local values of navigational factors into this map, it seems possible that such a process needs to occur only once at a given site, when the birds are visiting this site for the first time. If that were the case, the birds could interpret the local factors correctly at any later visit and orient by them. This hypothesis could explain the oriented behavior of birds at familiar sites, and it could also help to reconcile some of the seemingly controversial findings reported in the literature, where the effect of olfactory deprivation was reported to differ considerably between the various pigeon lofts, possibly because of different training procedures.
Development of UV-Curable Resins Suitable for Reverse-Tone Lithography for Au Metamaterials Using a Print-and-Imprint Method
Authors: Uehara Takuya , Sato Shinya , Ito Shunya , Yano Haruna , Nakamura Takahiro , Nakagawa Masaru 
Published: November 2017

Abstract
We developed a UV-curable resin (NL-SU1) suitable for screen printing with laser-drilled polyimide masks and reverse-tone nanoimprint lithography. The viscosity of the UV-curable resin composed of two bisphenol A-based monomers was adjusted to 11.0 Pa·s for the screen printing process. It was determined by photo-differential scanning calorimetry that photoinitiator Irgacure 369 was suitable for high methacrylate consumption in UV curing. The UV-curable resin after curing could be used as a top-coated resist layer on another imprinted resist layer because of its sufficient contrast in oxygen reactive ion etching and argon ion milling. We demonstrated a method for reverse-tone lithography in a print–and-imprint method to fabricate 20-nm-thick and 50-nm-linewide Au split-ring resonator arrays.
Two-Dimensional Array ATP/ADP Sensitive Image Sensor with a Uniform Distribution of Chemically Immobilized Apyrase
Authors: Endo Shinnosuke , Kato Ryo , Sawada Kazuaki , Hattori Toshiaki 
Published: November 2017

Abstract
ATP and ADP are the major energy source in metabolism of cells, and furthermore ATP works as an important extracellular signaling material. ATP bioimaging is required to understand their metabolism. In this paper, a label-free ATP/ADP image sensor was fabricated using a 128 × 128 (16 k) pixel array semiconductor CCD-type pH image sensor and Apyrase. The principle is based on measuring protons produced by the enzyme reaction between Apyrase and ATP or ADP. In order to put a uniform potential response of the sensor into practice, two different methods, (3-APTES and CEST), which chemically fixed N-terminal of Apyrase with the sensor were examined. The sensor modified by the CEST method had a quite clean surface microscopically and demonstrated a fine real-time image monitoring the ATP concentration. The potential response of the image sensor was characterized; effect of buffer solution, calibration curves of ATP and ADP, durability, the limit of detection (LOD) for ATP, and the response of time. The potential distribution for effective pixels to ATP concentration was narrow, single and symmetrical. Due to 16 K sensing pixels finely responding to ATP concentration uniformly, the LOD of ATP concentration (10 µM) was exactly determined with a statistical treatment.
Mandado de segurança - Processo legislativo - Legitimidade ativa
Authors: Mello Celso Antônio Bandeira de 
Published: November 2017

- O processo legislativo é suscetível de controle pelo Poder Judiciário havendo possibilidade de lesão à ordem jurídico-constitucional.- A legitimidade ativa é privativa de membro do Congresso Nacional, não cabendo a terceiros.