bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal sensitivity as a predictor of change in respiratory sinus arrhythmia activity from infancy to toddlerhood
Authors: Tacana Tracey , Speck Bailey , Isenhour Jennifer , Conradt Elisabeth , Crowell Sheila E. , Raby K. Lee 
Published: October 2024

AbstractThis study examined whether parental sensitivity during distressing and non‐distressing mother–infant interactions predicts changes in young children's respiratory sinus arrhythmia (RSA) activity. Baseline RSA levels were collected from 83 children (49% female, 51% male) when children were 7 and 18 months old. Children's RSA reactivity and RSA recovery during the still‐face paradigm were collected when children were 7 months and during the strange situation procedure at 18 months. Controlling for stability of RSA activity over time, maternal sensitivity during distressing interactions at 7 months predicted changes in children's baseline RSA levels (β = −0.30) and children's RSA recovery (β = 0.25). Young children who experienced higher levels of sensitivity at 7 months had lower resting RSA levels and exhibited greater RSA recovery at 18 months. These results suggest that changes in young children's RSA activity are meaningfully related to their early caregiving experiences.Highlights

We examined whether maternal sensitivity during infancy predicts changes in children's RSA activity from infancy to toddlerhood.
Children who experienced higher levels of maternal sensitivity during infancy showed greater RSA recovery from a stressor during toddlerhood.
Children who experienced higher levels of maternal sensitivity during infancy had lower resting RSA levels during toddlerhood.
US household demand system analysis for dairy milk products and plant‐based milk alternatives
Authors: Capps Oral , Wang Lingxiao 
Published: October 2024

AbstractUsing the QUAIDS model, we analyze interrelationships among dairy milk and plant‐based milk alternatives (PBMA) for US households from 2018 to 2020 using NielsenIQ. We adopt the Shonkwiler–Yen methodology to account for censored observations. PBMA demand is inelastic, while milk products show elastic demand. PBMA substitutes for traditional white milk and organic milk. Traditional flavored milk and PBMA as well as lactose‐free and organic milk are complements. PBMA and lactose‐free milk are independent goods. Demographic factors like income, household size, and education significantly affect budget share, alongside women, infants, and children participation and the pandemic.
An in vitro cell model for exploring inflammatory and amyloidogenic events in alkaptonuria
Authors: Mastroeni Pierfrancesco , Geminiani Michela , Olmastroni Tommaso , Frusciante Luisa , Trezza Alfonso , Visibelli Anna , Santucci Annalisa 
Published: October 2024

AbstractAlkaptonuria (AKU) is a progressive systemic inherited metabolic disorder primarily affecting the osteoarticular system, characterized by the degeneration of cartilage induced by ochronosis, ultimately leading to early osteoarthritis (OA). However, investigating AKU pathology in human chondrocytes, which is crucial for understanding the disease, encounters challenges due to limited availability and donor variability. To overcome this obstacle, an in vitro model has been established using homogentisic acid (HGA) to simulate AKU conditions. This model employed immortalized C20/A4 human chondrocytes and serves as a dependable platform for studying AKU pathogenesis. Significantly, the model demonstrates the accumulation of ochronotic pigment in HGA‐treated cells, consistent with findings from previous studies. Furthermore, investigations into inflammatory processes during HGA exposure revealed notable oxidative stress, as indicated by elevated levels of reactive oxygen species and lipid peroxidation. Additionally, the model demonstrated HGA‐induced inflammatory responses, evidenced by increased production of nitric oxide, overexpression of inducible nitric oxide synthase, and cyclooxygenase‐2. These findings underscore the model's utility in studying inflammation associated with AKU. Moreover, analysis of serum amyloid A and serum amyloid P proteins revealed a potential interaction, corroborating evidence of amyloid fibril formation. This hypothesis was further supported by Congo red staining, which showed fibril formation exclusively in HGA‐treated cells. Overall, the C20/A4 cell model provided valuable insights into AKU pathogenesis, emphasizing its potential for facilitating drug development and therapeutic interventions.
Tensile force impairs lip muscle regeneration under the regulation of interleukin‐10
Authors: Cheng Xu , Dou Jinfeng , Li Jinggui , Huang Yixuan , Shi Bing , Li Jingtao 
Published: October 2024

AbstractBackgroundOrbicularis oris muscle, the crucial muscle in speaking, facial expression and aesthetics, is considered the driving force for optimal lip repair. Impaired muscle regeneration remains the main culprit for unsatisfactory surgical outcomes. However, there is a lack of study on how different surgical manipulations affect lip muscle regeneration, limiting efforts to seek effective interventions.MethodsIn this study, we established a rat lip surgery model where the orbicularis oris muscle was injured by manipulations including dissection, transection and stretch. The effect of each technique on muscle regeneration was examined by histological analysis of myogenesis and fibrogenesis. The impact of tensile force was further investigated by the in vitro application of mechanical strain on cultured myoblasts. Transcriptome profiling of muscle satellite cells from different surgical groups was performed to figure out the key factors mediating muscle fibrosis, followed by therapeutic intervention to improve muscle regeneration after lip surgeries.ResultsEvaluation of lip muscle regeneration till 56 days after injury revealed that the stretch group resulted in the most severe muscle fibrosis (n = 6, fibrotic area 48.9% in the stretch group, P < 0.001, and 25.1% in the dissection group, P < 0.001). There was the lowest number of Pax7‐positive nuclei at Days 3 and 7 in the stretch group (n = 6, P < 0.001, P < 0.001), indicating impaired satellite cell expansion. Myogenesis was impaired in both the transection and stretch groups, as evidenced by the delayed peak of centrally nucleated myofibers and embryonic MyHC. Meanwhile, the stretch group had the highest percentage of Pdgfra+ fibro‐adipogenic progenitors infiltrated area at Days 3, 7 and 14 (n = 6, P = 0.003, P = 0.006, P = 0.037). Cultured rat lip muscle myoblasts exhibited impaired myotube formation and fusion capacity when exposed to a high magnitude (ε = 2688 μ strain) of mechanical strain (n = 3, P = 0.014, P = 0.023). RNA‐seq analysis of satellite cells isolated from different surgical groups demonstrated that interleukin‐10 was the key regulator in muscle fibrosis. Administration of recombinant human Wnt7a, which can inhibit the expression of interleukin‐10 in cultured satellite cells (n = 3, P = 0.041), exerted an ameliorating effect on orbicularis oris muscle fibrosis after stretching injury in surgical lip repair.ConclusionsTensile force proved to be the most detrimental manoeuvre for post‐operative lip muscle regeneration, despite its critical role in correcting lip and nose deformities. Adjunctive biotherapies to regulate the interleukin‐10‐mediated inflammatory process could facilitate lip muscle regeneration under conditions of high surgical tensile force.
AutoLNMNet: Automated Network for Estimating Lymph‐Node Metastasis in EGC Using a Pyramid Vision Transformer and Data Derived From Multiphoton Microscopy
Authors: Gao Lin , Liu Wenju , Kang Bingzi , Wu Han , He Jiajia , Li Xiaolu , Xi Gangqin , Zhuo Shuangmu 
Published: October 2024

ABSTRACTLymph‐node status is important in decision‐making during early gastric cancer (EGC) treatment. Currently, endoscopic submucosal dissection is the mainstream treatment for EGC. However, it is challenging for even experienced endoscopists to accurately diagnose and treat EGC. Multiphoton microscopy can extract the morphological features of collagen fibers from tissues. The characteristics of collagen fibers can be used to assess the lymph‐node metastasis status in patients with EGC. First, we compared the accuracy of four deep learning models (VGG16, ResNet34, MobileNetV2, and PVTv2) in training preprocessed images and test datasets. Next, we integrated the features of the best‐performing model, which was PVTv2, with manual and clinical features to develop a novel model called AutoLNMNet. The prediction accuracy of AutoLNMNet for the no metastasis (Ly0) and metastasis in lymph nodes (Ly1) stages reached 0.92, which was 0.3% higher than that of PVTv2. The receiver operating characteristics of AutoLNMNet in quantifying Ly0 and Ly1 stages were 0.97 and 0.97, respectively. Therefore, AutoLNMNet is highly reliable and accurate in detecting lymph‐node metastasis, providing an important tool for the early diagnosis and treatment of EGC.
若年者に発症したMRSAによる右心系three noes感染性心内膜炎の1例(‘Three noes’ right–sided infective endocarditis in a young patient due to methicillin–resistant Staphylococcus aureus: a case report)
Authors: 潤 中島 , 悠輔 澤田 , 雄太 一色 , 優美 市川 , 一憲 福島 , 祐斗 荒巻 , 清宏 大嶋 
Published: October 2024

要旨症例は20歳代の男性。発熱と頭痛を主訴に当院へ救急搬送された。来院時の血液検査で炎症反応上昇を認めるも，その他の検査で感染所見はなかった。感染巣不明の感染症を疑い，入院のうえ，抗菌薬治療を開始した。第2病日，血液培養からグラム陽性球菌が検出された。感染性心内膜炎（infective endocarditis: IE）の検索のため第3病日に経胸壁心エコー図検査を行うも疣贅はみられなかった。その後，検出菌がMRSAであることが判明し，第3病日に再検した血液培養検査でもMRSAが検出された。経過中に心雑音が出現・増強し，第9病日に行った経食道心エコー図検査で三尖弁位の疣贅と三尖弁閉鎖不全症を認めたためIEと診断し循環器内科へ転科した。抗菌化学療法が継続されたが，敗血症性肺塞栓症発症や炎症反応上昇再燃のため，第30病日に疣贅切除，三尖弁形成術および卵円孔閉鎖術が行われた。術後経過は良好で，第59病日に当院を退院した。右心系IEの主な誘因には，①静注薬物使用者，②心臓デバイス関連，③先天性心疾患，④上記①〜③のいずれでもないthree noes IEの4つがあり，本症例は市中獲得MRSAによるthree noes IEと考えられた。Three noes右心系IEの死亡率は高いため，迅速な抗菌化学療法開始とともに，治療戦略として外科治療も念頭に置きながら加療を進める必要がある。
Guselkumab for the treatment of pyoderma gangrenosum
Authors: Sarsam Sera , Sexton Holly , Nicolopoulos Jenny , Dolianitis Con 
Published: October 2024

AbstractPyoderma gangrenosum (PG) is a complex disease with limited therapeutic options. Here we report the use of higher dose of guselkumab, a monoclonal antibody targeting interleukin (IL‐) 23, in the treatment of PG in a medically complex patient. This case contributes additional evidence supporting the potential effectiveness of guselkumab in the treatment of PG and highlights the necessity for larger studies to confirm these findings.
Contrasting nitrogen dynamics across the Mid‐Atlantic Bight shelfbreak front: Insights from nitrate dual isotopes and nitrifier gene abundance
Authors: Zhu Yifan , Mulholland Margaret R. , Selden Corday R. , McGillicuddy Dennis J. , Mottram Josie , Chappell P. Dreux , Zhang Weifeng Gordon , Granger Julie , Crider Katherine E. , Meyer Meredith G. , Bernhardt Peter W. , Oliver Hilde , Clayton Sophie 
Published: October 2024

AbstractObservations and model studies suggest that front dynamics can enhance phytoplankton productivity. This study tested whether frontal systems also increase the abundance of nitrifying microbes and nitrogen recycling during repeat sampling transects across the Mid‐Atlantic Bight shelfbreak in July 2019. We measured ammonium concentrations, nitrate dual isotopes (δ15N, δ18O), and ammonia monooxygenase subunit A (amoA) genes of ammonia‐oxidizing archaea (AOA) and bacteria (AOB). In subsurface shelf waters, ammonium concentrations exceeded 2 μmol L−1, due to a temporary imbalance in regeneration from sinking particles and subsequent nitrification. The inverse correlation between nitrate δ15N values and ammonium concentrations confirmed nitrate was partially or entirely from local nitrification on the shelf. In contrast, the shelfbreak frontal zone and slope sea subsurface waters had much lower ammonium concentrations (0.1–0.2 μmol L−1) due to tight coupling between ammonium regeneration and nitrification. The deviation of nitrate δ15N and δ18O from algal uptake‐driven 1 : 1 ratio suggests concurrent nitrification in the euphotic zone. The shelfbreak front acted as an ecological boundary where AOA and AOB amoA gene numbers were partitioned, with AOAs abounding in slope waters and AOBs in shelf waters, likely due to ammonium availability. At certain slope stations, deep‐water nutrient inputs via isopycnal lifting induced by Gulf Stream intrusions caused unexpectedly high phytoplankton biomass, which doubled nitrifier abundance and potentially stimulated both ammonium regeneration and nitrification. These findings demonstrate distinct distributions of nitrifying microbes along the salinity gradient from shelf to slope and highlight the significant influence of coastal ocean‐western boundary current interactions on nitrogen biogeochemistry.
EmbB and EmbC regulate the sensitivity of Mycobacterium abscessus to echinomycin
Authors: He Jing , Gao Yamin , Wang Jingyun , Hameed H. M. Adnan , Wang Shuai , Fang Cuiting , Tian Xirong , Zhang Jingran , Han Xingli , Ju Yanan , Tan Yaoju , Ma Junying , Ju Jianhua , Hu Jinxing , Liu Jianxiong , Zhang Tianyu 
Published: October 2024

AbstractTreatment of Mycobacterium abscessus (Mab) infections is very challenging due to its intrinsic resistance to most available drugs. Therefore, it is crucial to discover novel anti‐Mab drugs. In this study, we explored an intrinsic resistance mechanism through which Mab resists echinomycin (ECH). ECH showed activity against Mab at a minimum inhibitory concentration (MIC) of 2 µg/ml. A ΔembC strain in which the embC gene was knocked out showed hypersensitivity to ECH (MIC: 0.0078–0.0156 µg/ml). The MICs of ECH‐resistant strains screened with reference to ΔembC ranged from 0.25 to 1 µg/ml. Mutations in EmbB, including D306A, D306N, R350G, V555I, and G581S, increased the Mab's resistance to ECH when overexpressed in ΔembC individually (MIC: 0.25–0.5 µg/ml). These EmbB mutants, edited using the CRISPR/Cpf1 system, showed heightened resistance to ECH (MIC: 0.25–0.5 µg/ml). The permeability of these Mab strains with edited genes and overexpression was reduced, as evidenced by an ethidium bromide accumulation assay, but it remained significantly higher than that of the parent Mab. In summary, our study demonstrates that ECH exerts potent anti‐Mab activity and confirms that EmbB and EmbC are implicated in Mab's sensitivity to ECH. Mutation in EmbB may partially compensate for a loss of EmbC function.
Zinc finger 4 negatively controls the transcriptional activator Fzf1 in Saccharomyces cerevisiae
Authors: Du Ying , Ma Chaoqun , Moore Stanley A. , Xiao Wei 
Published: October 2024

AbstractFzf1 is a Saccharomyces cerevisiae transcription factor containing five zinc fingers (ZFs). It regulates the expression of at least five downstream genes, including SSU1, YHB1, DDI2/3, and YNR064c, by recognizing a consensus sequence, CS2, found in these gene promoters. These gene products are involved in cellular responses to various chemical stresses. For example, SSU1 encodes a sodium sulfite efflux protein that confers sulfite resistance. However, the underlying molecular mechanism through which Fzf1 responds to chemical stress and coordinates target gene activation remains elusive. Interestingly, several mutations in the fourth ZF (ZF4) of Fzf1 have previously been reported to confer either sulfite resistance or elevated basal‐level expression of YHB1, indicating that ZF4 negatively impacts Fzf1 activity. Since ZF4 is dispensable for CS2 binding in vitro, we hypothesized that ZF4 is a negative regulator of Fzf1 and that chemically induced Fzf1‐regulated gene expression occurs via de‐repression. All five genes examined were cross‐induced by corresponding chemicals in an Fzf1‐dependent manner, and all three ZF4 mutations and a ZF4 deletion conferred increased basal‐level expression and SSU1‐dependent sulfite resistance. A ZF4 deletion did not alter the target DNA binding, consistent with the observed codominant phenotype. These observations collectively reveal that Fzf1 remains inactive by default at the target promoters and that its activation is at least partially achieved by self‐derepression through chemical modification and/or a conformational change.
Monolithic ceramic waveguide filter using a transition between GCPW feed and ceramic waveguide for 28 GHz
Authors: Park Jeong‐Hun , Park Seung‐Jun 
Published: October 2024

AbstractIn this letter, a mm‐wave monolithic ceramic waveguide filter using a transition between a grounded coplanar waveguide (GCPW) feed and a ceramic waveguide has been proposed. The published studies on monolithic ceramic waveguide filters with wideband properties for the mm‐wave are lacking. A desired external quality factor, satisfying wide bandwidth, is achieved using both inductive and capacitive couplings in the transition. The inductive coupling is realized with a waveguide's hole directly connected to the microstrip line probe of the GCPW. The capacitive coupling is employed with the slots of both GCPW and ceramic waveguide, which are placed facing each other. Adopting the presented transition, the inline monolithic ceramic waveguide filter for a fourth‐order Chebyshev response was designed. The fabricated filter provided the insertion and return losses of lower than 1.2 dB and higher than 10 dB, respectively, at frequencies from 26.43 to 29.76 GHz, with a fractional bandwidth of 11.85%.
Physical Activity, Kinesiophobia, Pain Catastrophizing, Body Awareness, Depression and Disease Activity in Patients With Ankylosing Spondylitis and Rheumatoid Arthritis: A Cross‐Sectional Explorative Study
Authors: Karaca Nur Banu , Arin‐Bal Gamze , Sezer Serdar , Kelesoglu Dincer Ayse Bahar , Kinikli Gulay , Boström Carina , Kinikli Gizem Irem 
Published: October 2024

ABSTRACTObjective/AimThe aim was to compare the levels of physical activity, kinesiophobia, pain catastrophizing, body awareness, and depression in patients with Ankylosing Spondylitis (AS) and Rheumatoid Arthritis (RA) and to explore the associations between these outcomes and disease activity.MethodsSeventy‐eight patients with AS (n = 30) and RA (n = 48) were included. Outcomes were assessed using the International Physical Activity Questionnaire‐Short Form, the Tampa Scale of Kinesiophobia, the Pain Catastrophizing Scale, the Body Awareness Questionnaire, and the Beck Depression Inventory. Disease activity levels were determined using the Bath Ankylosing Spondylitis Disease Activity Index for AS and the Disease Activity Score 28 score for RA.ResultsAS patients were younger, had a higher proportion of men, and were more physically active than RA patients (p < 0.05). Both groups exhibited high levels of kinesiophobia but low levels of pain catastrophizing, similar body awareness and mild depression scores. Moderate correlations (r ≥ 0.5) were observed between kinesiophobia and depression, body awareness and pain catastrophizing and depression, and pain catastrophizing and disease activity in AS patients. In RA patients, moderate correlations (r ≥ 0.5) were found between kinesiophobia and pain catastrophizing.ConclusionAddressing physical activity, kinesiophobia, pain catastrophizing, body awareness, and depression is important in managing AS and RA patients. Notably, correlations among outcomes differed between groups, with more significant correlations in AS. Further studies are needed to explore these in greater detail.
Assessing Long‐Term Quality of Life Concerns Through Survivorship Clinic in Head and Neck Cancer Patients
Authors: Yousef Andrew , Nichol Ariadne , Luu Bao , Montesa Carie , Ramsey Celia , Verpukhovskiy Philipp , Blumenfeld Liza , Linnemeyer‐Risser Kristen , Guo Theresa 
Published: October 2024

AbstractObjectiveTo characterize the concerns of head and neck cancer (HNC) patients and discern changes in quality‐of‐life (QoL) during long‐term follow‐up.Study DesignRetrospective review.SettingSurvivorship clinic at a tertiary academic center.MethodsA retrospective review was conducted on HNC patients seen in our survivorship clinic between 1/2020 and 1/2024 using the University of Washington Quality of Life (UW‐QOL) Questionnaire.ResultsThree hundred and forty‐two patients were seen for 914 encounters. Patients were divided into 4 groups: pretreatment (n = 326), 0 to 12 months posttreatment (n = 247), 1 to 3 years posttreatment (n = 248), and more than 3 years posttreatment (n = 64). The average follow‐up after treatment was 459 days (range: 0‐5.2 years).Multivariable analysis revealed significant improvements in overall QoL, health‐related QoL, social‐emotional composite scores, activity, anxiety, appearance, chewing, mood, pain, speech, and recreation at more than 1‐year posttreatment compared to less than 1‐year posttreatment. However, declines were noted in saliva and taste scores. No differences in scores were observed between patients 1 to 3 years posttreatment and those >3 years posttreatment.At all timepoints before and after treatment, top concerns were pain, activity, and swallowing. Patients with oral cancer followed for more than 1‐year posttreatment had worse scores in appearance, chewing, pain, and speech compared to those with oropharyngeal cancer.ConclusionsUnderstanding the evolving concerns of HNC patients is imperative for enhancing care. Most QoL domains improve at 1‐year posttreatment except for saliva, taste, swallowing, and shoulder function. QoL scores stabilize after 1‐year post‐treatment. Pain, activity, and swallowing remain the top concerns at all time points.
Esculin Alleviates Osteoarthritis Progression Through the Sirt1/NF‐κB Pathway
Authors: Sun Tao , Bai Bingli , Wu Haohao , Xing Hailin , Li Jian 
Published: October 2024

ABSTRACTOsteoarthritis (OA), a joint disease associated with inflammatory processes, contributes to joint destruction. Esculin (ESC) extracted from the stem bark of Fraxinus rhynchophylla Hance has been shown to possess anti‐inflammatory properties. In this study, we investigated the effect of ESC on chondrocytes treated with IL‐1β and its molecular mechanism. The importance and potential mechanism of ESC in the progression of OA were evaluated. The viability of chondrocytes after exposure to ESC was examined through the CCK‐8 assays. The cells were then subjected to quantitative polymerase chain reaction (qPCR), western blot, and enzyme‐linked immunosorbent assay (ELISA) techniques to analyze the degradation of the extracellular matrix (ECM) and occurrence of inflammation. The NF‐κB mechanism was evaluated by western blot analysis, immunofluorescence (IF), and luciferase reporter assay. Molecular docking was performed to allow for predictions on proteins that interact with ESC. Moreover, the significance of Sirt1 was explored through a knockdown experiment based on siRNA. Micro‐computed tomography (CT), H&E, Safranin O‐Fast Green (S‐O), and immunohistochemical analyses were carried out to assess the treatment efficacy of ESC on OA in destabilization of medial meniscus (DMM) models. ESC treatment effectively inhibited ECM degradation, modulated the levels of pro‐inflammatory factors, and regulated the NF‐κB signaling in chondrocytes exposed to IL‐1β. Mechanistically, we found that ESCs bound to Sirt1 to inhibit the activity of the NF‐κB mechanism. Furthermore, ESC treatment suppressed OA progression in the DMM models. Our findings reveal that ESC ameliorates OA progression via modulating the Sirt1/NF‐κB axis. This demonstrates that ESC has the potential to be applied in the treatment of OA.