bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes
Authors: Hu Yuan-Lang , Mi Xue , Huang Chao , Wang Hui-Fang , Song Jian-Ren , Shu Qing , Ni Lan , Chen Jian-Guo , Wang Fang , Hu Zhuang-Li 
Published: March 2017

AbstractAcidosis has been known to cause “Ca2+ transients”, however, the mechanism is still uncertain. Here, we demonstrated that multiple H+ sensors, such as ASICs, TRPV1 and proton-sensing G protein coupled receptors (GPCRs) are involved in extracellular acidification-induced intracellular calcium ([Ca2+]i) elevation. By using calcium imaging measures, we observed that both ASIC and TRPV1 channels inhibitors suppressed the [Ca2+]i elevation induced by extracellular acidosis in cultured rat cardiac myocytes. Then, both channels mRNA and proteins were identified by RT-PCR, western blotting and immunofluorescence. ASIC-like and TRPV1-like currents were induced by extracellular acidification, suggesting that functional ASIC and TRPV1 channels jointly mediated extracellular calcium entry. Furthermore, either pre-exhaustion of sarcoplasmic reticulum (SR) Ca2+ with thapsigargin or IP3 receptor blocker 2-APB or PLC inhibitor U73122 significantly attenuated the elevation of [Ca2+]i, indicating that the intracellular Ca2+ stores and the PLC-IP3 signaling also contributed to the acidosis-induced elevation of [Ca2+]i. By using genetic and pharmacological approaches, we identified that ovarian cancer G protein-coupled receptor 1 (OGR1) might be another main component in acidosis-induced release of [Ca2+]i. These results suggest that multiple H+-sensitive receptors are involved in “Ca2+ transients” induced by acidosis in the heart.
Elevated miR-130a/miR130b/miR-152 expression reduces intracellular ATP levels in the pancreatic beta cell
Authors: Ofori Jones K. , Salunkhe Vishal A. , Bagge Annika , Vishnu Neelanjan , Nagao Mototsugu , Mulder Hindrik , Wollheim Claes B. , Eliasson Lena , Esguerra Jonathan L. S. 
Published: March 2017

AbstractMicroRNAs have emerged as important players of gene regulation with significant impact in diverse disease processes. In type-2 diabetes, in which impaired insulin secretion is a major factor in disease progression, dysregulated microRNA expression in the insulin-secreting pancreatic beta cell has been widely-implicated. Here, we show that miR-130a-3p, miR-130b-3p, and miR-152-3p levels are elevated in the pancreatic islets of hyperglycaemic donors, corroborating previous findings about their upregulation in the islets of type-2 diabetes model Goto-Kakizaki rats. We demonstrated negative regulatory effects of the three microRNAs on pyruvate dehydrogenase E1 alpha (PDHA1) and on glucokinase (GCK) proteins, which are both involved in ATP production. Consequently, we found both proteins to be downregulated in the Goto-Kakizaki rat islets, while GCK mRNA expression showed reduced trend in the islets of type-2 diabetes donors. Overexpression of any of the three microRNAs in the insulin-secreting INS-1 832/13 cell line resulted in altered dynamics of intracellular ATP/ADP ratio ultimately perturbing fundamental ATP-requiring beta cell processes such as glucose-stimulated insulin secretion, insulin biosynthesis and processing. The data further strengthen the wide-ranging influence of microRNAs in pancreatic beta cell function, and hence their potential as therapeutic targets in type-2 diabetes.
Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel
Authors: Cochis A. , Grad S. , Stoddart M. J. , Farè S. , Altomare L. , Azzimonti B. , Alini M. , Rimondini L. 
Published: March 2017

AbstractAutologous chondrocyte implantation for cartilage repair represents a challenge because strongly limited by chondrocytes’ poor expansion capacity in vitro. Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. Moreover, MSC supporting material selection is fundamental to allow for an active interaction with cells. Here, we tested a novel thermo-reversible hydrogel composed of 8% w/v methylcellulose (MC) in a 0.05 M Na2SO4 solution. MC hydrogel was obtained by dispersion technique and its thermo-reversibility, mechanical properties, degradation and swelling were investigated, demonstrating a solution-gelation transition between 34 and 37 °C and a low bulk degradation (<20%) after 1 month. The lack of any hydrogel-derived immunoreaction was demonstrated in vivo by mice subcutaneous implantation. To induce in vitro chondrogenesis, MSCs were seeded into MC solution retained within a porous polyurethane (PU) matrix. PU-MC composites were subjected to a combination of compression and shear forces for 21 days in a custom made bioreactor. Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis.
Brain anatomy alterations associated with Social Networking Site (SNS) addiction
Authors: He Qinghua , Turel Ofir , Bechara Antoine 
Published: March 2017

AbstractThis study relies on knowledge regarding the neuroplasticity of dual-system components that govern addiction and excessive behavior and suggests that alterations in the grey matter volumes, i.e., brain morphology, of specific regions of interest are associated with technology-related addictions. Using voxel based morphometry (VBM) applied to structural Magnetic Resonance Imaging (MRI) scans of twenty social network site (SNS) users with varying degrees of SNS addiction, we show that SNS addiction is associated with a presumably more efficient impulsive brain system, manifested through reduced grey matter volumes in the amygdala bilaterally (but not with structural differences in the Nucleus Accumbens). In this regard, SNS addiction is similar in terms of brain anatomy alterations to other (substance, gambling etc.) addictions. We also show that in contrast to other addictions in which the anterior-/ mid- cingulate cortex is impaired and fails to support the needed inhibition, which manifests through reduced grey matter volumes, this region is presumed to be healthy in our sample and its grey matter volume is positively correlated with one’s level of SNS addiction. These findings portray an anatomical morphology model of SNS addiction and point to brain morphology similarities and differences between technology addictions and substance and gambling addictions.
Injections through skin colonized with Staphylococcus aureus biofilm introduce contamination despite standard antimicrobial preparation procedures
Authors: Wang Yi , Leng Valery , Patel Viraj , Phillips K. Scott 
Published: March 2017

AbstractWhile surgical site preparation has been extensively studied, there is little information about resistance of skin microbiota in the biofilm form to antimicrobial decontamination, and there are no quantitative models to study how biofilm might be transferred into sterile tissue/implant materials during injections for joint spine and tendon, aspiration biopsies and dermal fillers (DF). In this work, we develop twoin vitromodels to simulate the process of skin preparation and DF injection using pig skin and SimSkin (silicone) materials, respectively. Using the pig skin model, we tested three of the most common skin preparation wipes (alcohol, chlorhexidine and povidone iodine) and found that during wiping they reduced the biofilm bacterial burden ofS. aureus(CFU cm−2) by three logs with no statistically significant differences between wipes. Using the SimSkin model, we found that transfer of viable bacteria increased with needle diameter for 30G, 25G and 18G needles. Transfer incidence decreased as injection depth was increased from 1 mm to 3 mm. Serial puncture and linear threading injection styles had similar transfer incidence, whereas fanning significantly increased transfer incidence. The results show that contamination of DF during injection is a risk that can be reduced by modifying skin prep and injection practices.
Akt targeting as a strategy to boost chemotherapy efficacy in non-small cell lung cancer through metabolism suppression
Authors: Le Grand Marion , Berges Raphael , Pasquier Eddy , Montero Marie-Pierre , Borge Laurence , Carrier Alice , Vasseur Sophie , Bourgarel Veronique , Buric Duje , André Nicolas , Braguer Diane , Carré Manon 
Published: March 2017

AbstractMetabolic reprogramming is a hallmark of cancer development, mediated by genetic and epigenetic alterations that may be pharmacologically targeted. Among oncogenes, the kinase Akt is commonly overexpressed in tumors and favors glycolysis, providing a rationale for using Akt inhibitors. Here, we addressed the question of whether and how inhibiting Akt activity could improve therapy of non-small cell lung cancer (NSCLC) that represents more than 80% of all lung cancer cases. First, we demonstrated that Akt inhibitors interacted synergistically with Microtubule-Targeting Agents (MTAs) and specifically in cancer cell lines, including those resistant to chemotherapy agents and anti-EGFR targeted therapies. In vivo, we further revealed that the chronic administration of low-doses of paclitaxel - i.e. metronomic scheduling - and the anti-Akt perifosine was the most efficient and the best tolerated treatment against NSCLC. Regarding drug mechanism of action, perifosine potentiated the pro-apoptotic effects of paclitaxel, independently of cell cycle arrest, and combining paclitaxel/perifosine resulted in a sustained suppression of glycolytic and mitochondrial metabolism. This study points out that targeting cancer cell bioenergetics may represent a novel therapeutic avenue in NSCLC, and provides a strong foundation for future clinical trials of metronomic MTAs combined with Akt inhibitors.
TRPM8 in the negative regulation of TNFα expression during cold stress
Authors: Wang Xin-Pei , Yu Xuan , Yan Xiao-Jin , Lei Fan , Chai Yu-Shuang , Jiang Jing-Fei , Yuan Zhi-Yi , Xing Dong-Ming , Du Li-Jun 
Published: March 2017

AbstractTransient Receptor Potential Melastatin-8 (TRPM8) reportedly plays a fundamental role in a variety of processes including cold sensation, thermoregulation, pain transduction and tumorigenesis. However, the role of TRPM8 in inflammation under cold conditions is not well known. Since cooling allows the convergence of primary injury and injury-induced inflammation, we hypothesized that the mechanism of the protective effects of cooling might be related to TRPM8. We therefore investigated the involvement of TRPM8 activation in the regulation of inflammatory cytokines. The results showed that TRPM8 expression in the mouse hypothalamus was upregulated when the ambient temperature decreased; simultaneously, tumor necrosis factor-alpha (TNFα) was downregulated. The inhibitory effect of TRPM8 on TNFα was mediated by nuclear factor kappa B (NFκB). Specifically, cold stress stimulated the expression of TRPM8, which promoted the interaction of TRPM8 and NFκB, thereby suppressing NFκB nuclear localization. This suppression consequently led to the inhibition of TNFα gene transcription. The present data suggest a possible theoretical foundation for the anti-inflammatory role of TRPM8 activation, providing an experimental basis that could contribute to the advancement of cooling therapy for trauma patients.
Microstructure formation and interface characteristics of directionally solidified TiAl-Si alloys in alumina crucibles with a new Y2O3 skull-aided technology
Authors: Fan Jianglei , Liu Jianxiu , Wu Shen , Tian Shuxia , Gao Hongxia , Wang Shengyong , Guo Jingjie , Wang Xiao 
Published: March 2017

AbstractThe microstructure evolution and interface characteristics of a directionally solidified Ti-43Al-3Si (at.%) alloy in an alumina (Al2O3) crucible with new Y2O3 skull-aided technology were investigated. The Y2O3-skull that is in contact with the TiAl-melt is relatively stable, which results in a more controlled reaction between the skull and the melt than in the case of an Al2O3 crucible is used. A thin reaction layer was formed between the mould and the melt through mutual diffusion. The layer thickness increased with increasing reaction time. The thickness of this layer was less than 80 μm for reaction times up to 5800 s. Y2O3 particles were not found in the specimen because the mould coating was prepared with fine Y2O3 powder without a binder, which prevented the Y2O3 particles splitting from the coating as a consequence of thermal physical erosion. The oxygen content of the TiAl-alloy increased with increasing reaction time. The total oxygen content of the solidified specimen was less than that of the specimen solidified in the Al2O3 crucibles. This new Y2O3 skull-aided technology is expected to improve the surface quality of TiAl-alloys and reduce the reaction between the crucible/mould and molten TiAl alloys during directional solidification processing with longer contact times.
Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections
Authors: He Hui , Li Rongqun , Chen Yi , Pan Ping , Tong Wenjuan , Dong Xueyan , Chen Yueming , Yu Daojun 
Published: March 2017

AbstractCurrent extraction methods often extract DNA and RNA separately, and few methods are capable of co-extracting DNA and RNA from sputum. We established a nucleic acid co-extraction method from sputum based on magnetic beads and optimized the method by evaluating influencing factors, such as the guanidinium thiocyanate (GTC) and dithiothreitol (DTT) concentrations, magnetic bead amount, incubation temperature, lysis buffer pH and RNA carrier type. The feasibility of the simultaneous nucleic acid co-extraction method was evaluated by amplifying DNA and RNA viruses from a single clinical specimen with a multiplex RT-qPCR method. Both DNA and RNA were most efficiently extracted when the GTC and DTT concentrations were 2.0 M and 80 mM, respectively, 20 μl magnetic beads were added, the incubation temperature was 80 °C, the pH was 8 or 9, and RNA carrier A was used. Therefore, we established a simple method to extract nucleic acids from two important respiratory viruses compared with other commercial kits. This magnetic beads-based co-extraction method for sputum followed by a multiplex RT-qPCR can rapidly and precisely detect DNA and RNA viruses from a single clinical specimen and has many advantages, such as decreased time, low cost, and a lack of harmful chemicals.
Long-Term Health-Related Quality of Life Outcomes in Digital Replantation versus Revision Amputation
Authors: Bartow Matthew , Tremblay-Champagne Marie , Lin Alex , Landes Geneviève , Sebbag Sarah , Nikolis Andreas , Tessler Oren 
Published: March 2017

Background Earlier, digit viability judged the success of digital replantation. Now, utility health-related quality of life (HRQOL) measures can better assess the impact of digital replantation.
Methods Overall, 264 digital injury patients were sent a regimen of utility measures: Disabilities of the Arm, Shoulder and Hand (DASH) score, European Quality of Life 5 Dimensions, visual analog scale (VAS), time trade-off (TTO), and standard gamble (SG). Overall, 51 patients responded completely to all of these—36 replantation patients and 15 revision amputation patients. The utility results of these patients were stratified between replantation versus revision amputation; dominant hand replantation versus nondominant hand replantation; and dominant hand revision amputation versus nondominant hand revision amputation.
Results The mean VAS score of replant (0.84) and revision amputation (0.75) groups was significantly different (p = 0.05). The mean DASH score of dominant hand replantations (29.72) and nondominant hand replantations (17.97) was significantly different (p = 0.027). The dominant hand revision amputation had higher anxiety levels in comparison to nondominant hand revision amputation (p = 0.027). Patients with two or more digits replanted showed a significant decrease in VAS, TTO, and SG scores in comparison to patients who only had one digit replanted (p = 0.009, 0.001, and 0.001, respectively).
Conclusions This study suggests that HRQOL can offer better indices for outcomes of digital replantation. This shows some specific replantation cohorts have a significantly better quality of life when compared with their specific correlating revision amputation cohort. These findings can be employed to further refine indications and contraindications to replantation and help predict the quality of life outcomes.
Playable Bodies
Authors: Miller Kiri 
Published: March 2017

Abstract
Playable Bodies investigates what happens when machines teach humans to dance. Dance videogames work as engines of humor, shame, trust, and intimacy, urging players to dance like nobody’s watching—while being tracked by motion-sensing interfaces in their living rooms. The chart-topping dance game franchises Just Dance and Dance Central transform players’ experiences of popular music, invite experimentation with gendered and racialized movement styles, and present new possibilities for teaching, learning, and archiving choreography. The book shows how these games teach players to regard their own bodies as both interfaces and avatars, and how a convergence of choreography and programming code is driving a new wave of full-body virtual-reality media experiences. Drawing on five years of ethnographic research with players, game designers, and choreographers, Playable Bodies situates dance games in a media ecology that includes the larger game industry, viral music videos, reality TV competitions, marketing campaigns, consumer reviews, social media discourse, and emerging surveillance technologies. Miller tracks the circulation of dance gameplay and related “body projects” across media platforms to reveal how dance games function as “intimate media,” configuring new relationships among humans, interfaces, music and dance repertoires, and social media practices.
Listening Like a Dancer
Authors: Miller Kiri 
Published: March 2017

Abstract
This chapter addresses multisensory musicality in dance games, situating gameplay as a form of embodied popular music reception that intersects with other kinds of musical experience and social media practice. Dance games capitalize on players’ prior relationships with particular songs, artists, and genres. They offer curated collections of licensed popular music tracks, and their dance routines are kinesthetic transcriptions of these musical works. Game choreographers employ specific listening techniques to create these routines; players report that performing game choreography transforms their listening experience. The chapter focuses on three aspects of interactive audio in dance games: how game developers turn song into dance, how players learn to listen like choreographers, and how gameplay offers experiential lessons in multisensory musicality by engaging players in proprioceptive interaction with sound. Interactive audio in dance games serves to cultivate a choreographic listening sensibility, bringing the listening ear and the moving body into intimate alignment.
Intimate Media
Authors: Miller Kiri 
Published: March 2017

Abstract
This chapter addresses dance games as “intimate media,” showing how they configure intimate relationships among humans, interfaces, musical and choreographic repertoires, and other social media practices. It guides readers through my research experience: navigating social media feeds, following chains of related videos, reading scholarly literature alongside comment threads, and learning to listen for concord, discord, echoes, and rhymes across these materials. The chapter presents six portraits of collective body projects mobilized via social media, placing dance games in the context of a broader media ecology that includes user-generated content, advertising campaigns, commercial music videos, mobile apps, and viral videos. These six body projects—uploading, disciplining, infringing, autodancing, belonging, and circulating—show us players negotiating the dynamics of control and consent that drive so many interactive media experiences today.
Characteristics of the VARHS Data and Other Data Sources
Authors: Brandt Kasper , Tarp Finn 
Published: March 2017

Abstract
The VARHS was first implemented in 2002 when it was realized that more information on household resources and the interaction of resources was needed in Viet Nam. From 2006 and onwards, households from twelve provinces are surveyed biennially and data collected. The studies of this book almost exclusively rely on households that are present in all VARHS datasets between 2006 and 2014, which covers 2,162 households being interviewed five times. The sample of VARHS households resembles, to a large extent, the samples in the representative VHLSS datasets and the 2009 population census. This implies that the results put together in this book are likely to apply to a more extensive part of the Vietnamese population than initially planned for.
Local Antibiotic Therapy to Reduce Infection After Operative Treatment of Fractures at High Risk of Infection: A Multicenter, Randomized, Controlled Trial (VANCO Study)
Authors: O'Toole Robert V. , Joshi Manjari , Carlini Anthony R. , Murray Clinton K. , Allen Lauren E. , Scharfstein Daniel O. , Gary Joshua L. , Bosse Michael J. , Castillo Renan C. ,   METRC
Published: March 2017

Summary:
A number of clinical studies in the spine literature suggest that the use of local vancomycin powder may substantially reduce surgical site infections (SSIs). These studies are primarily retrospective and observational and few focus on orthopaedic trauma patients. This study is a phase III, prospective, randomized, clinical trial to assess the efficacy of locally administered vancomycin powder in the prevention of SSI after fracture surgery. The primary goal of the VANCO Study is to compare the proportion of deep SSI 6 months after fracture fixation surgery. A secondary objective is to compare species and antibacterial susceptibilities among study patients who develop SSI. An additional objective is to build and validate a risk prediction model for the development of SSI. The study population consists of patients aged 18–80 years with tibial plateau or pilon (tibial plafond) fractures, at higher risk of infection, and definitively treated with plate and screw fixation. Participants are block randomized (within center) in a 1:1 ratio to either treatment group (local vancomycin powder up to a maximum dose of 1000 mg, placed immediately before wound closure) or control group (standard of care) for each study injury location, and return to the clinic for evaluations at 2 weeks, 3 months, and 6 months after fixation. The targeted sample size for the study is 500 fractures per study arm. This study should provide important information regarding the use of local vancomycin powder during the definitive treatment of lower extremity fractures and has the potential to significantly reduce the incidence of infection after orthopaedic trauma.