bluuebunny/crossref_metadata_2025_split huggingface dataset:
New RP-HPLC Method for Separation of Naja haje haje Venom and Studies of its Bactericidal Effect
Authors: Gâz Florea Şerban Andrei , Ciurca Diana , Mare Anca , Man Adrian , Cordoş Bogdan , Grama Anda-Lavinia , Muntean Daniela-Lucia 
Published: February 2016

Abstract
Background: Snake venom is a complex mixture of biologically active substances. Some peptides and proteins from snake have already demonstrated their therapeutically potential. The venom of Naja haje, an Elapidae member, has been analyzed from this point of view. Understanding the fully biochemical role of its enzymes has determined the scientists to find new separation and identification methods.
Objective: Our goal was to develop an optimal HPLC analytical method for separation and identification of Naja haje snake venom components, known for its neurotoxic activity. In addition, we wanted to find out if crude snake venom could inhibit the development of both Gram-positive and Gram-negative bacterial cultures. Materials and Method: Analysis of venom was performed on a HPLC system using a C16 column with UV detection at 210 nm. The analysis was done using two mobile phases, containing different concentrations of acetonitrile and trifluoroacetic acid aqueous solution at different pH values. An elution gradient was set at a flow of 0.60 mL/min. Bactericidal activity was quantified by measuring inhibition diameter around an aseptically disk filled with crude venom using Staphylococcus aureus and Escherichia coli. Results: An optimal HPLC analytical method has been developed by changing different parameters such as the pH value of mobile phase A or the elution gradient. The best resolution were obtained at a pH value of 7.4, in gradient varying from 5% to 45% in mobile phase B. Microbiological studies of the venom showed that Gram-positive bacteria growth was inhibited by crude venom, while on Gram-negative bacteria growth no effect was observed. Inhibition zone is dose-dependent and fresh crude venom is with 30% more potent than venom freeze and kept at -55°C. Conclusions: A comprehensive catalog of venom composition may serve as a starting point for studying structurefunction correlations of individual toxins for the development of new research tools and drugs of potential clinical use.
Multiparasitic Infection (Hydatid Cyst of the Spleen and Ascaris Lumbricoides Infestation) Mimicking an Acute Surgical Abdomen – Case Report
Authors: Molnar Călin , Vizitiu Alexandru , Deak Karoly , Russu Cristian , Tudor Adrian , Suciu Bogdan , Stolnicu Simona , Hulub Marius , Molnar Claudiu 
Published: February 2016

Abstract
Hydatid cyst involving the spleen is a rare clinical condition (0.5-4%). We report a case of multi-parasitic infection in a 62 year old female (hydatid cyst and ascaris lumbricoides infestation), confirmed during surgery. The purpose of the paper is to emphasize on the rare association of the two parasitic infections, affecting two different organs (spleen and jejunum). The combined symptomatology of the two parasites could mimic an acute surgical abdomen.
Investigating the Possibility to Individualize Asthma Attack Therapy Based on Attack Severity and Patient Characteristics
Authors: Sárkány Zoltán , Sárkány Kinga , Horváth A. 
Published: February 2016

Abstract
Introduction: The objective of this study was to investigate with the help of a computerized simulation model whether the treatment of an acute asthma attack can be individualized based on the severity of the attack and the characteristics of the patient. Material and Method: A stochastic lung model was used to simulate the deposition of 1 nm - 10 μm particles during a mild and a moderate asthma attack. Breathing parameters were varied to maximize deposition, and simulation results were compared with those obtained in the case of a severe asthma attack. In order to investigate the effect of height on the deposition of inhaled particles, another series of simulations was carried out with identical breathing parameters, comparing patient heights of 155 cm, 175 cm and 195 cm. Results: The optimization process yielded an increase in the maximum deposition values of around 6-7% for each type of investigated asthma attack, and the difference between attacks of different degree of severity was around 5% for both the initial and the optimized values, a higher degree of obstruction increasing the amount of deposited particles. Conclusions: Our results suggest that the individualization of asthma attack treatment cannot be based on particles of different size, as the highest deposited fraction in all three types of attacks can be obtained using 0.01 μm particles. The use of a specific set of breathing parameters yields a difference between a mild and a moderate, as well as a moderate and a severe asthma attack of around 5%.
Inferior Vena Cava Hypoplasia Associated with Deep Vein Thrombosis – Case Presentation
Authors: Nyulas Tiberiu , Blendea Ciprian , Rat Nora , Barcan Andreea , Korodi Szilamer , Bajka Balazs 
Published: February 2016

Abstract
Introduction: We present the case of a patient suffering from inferior vena cava hypoplasia complicated with Phlegmasia cerulean dolens. Imaging techniques allow precise diagnosis of inferior vena cava hypoplasia, providing essential structural details on the degree of damage of the vena cava and for the other branches. Case presentation: A 58 years old, obese and diabetic male patient presented with intense pain in the lower limbs, with the onset 24h before presentation. The patient presented generalized edema, cyanosis and functional impotence. Angio CT examination revealed hypoplasia of the inferior vena cava, with extensive DVT (deep vein thrombosis). In emergency conditions, with the agreement of the patient, we initiated the thrombolytic therapy (streptokinase for 72h) associated with anticoagulants (heparin). The evolution was favorable: a significant reduction in leg circumference was recorded, together with pain relief and reduction of local inflammation in the lower limbs. Conclusion: Severe cases of inferior vena cava hypoplasia complicated with deep vein thrombosis can present a good prognosis if appropriate treatment with anticoagulants and thrombolytics is initiated in time.
The solutions of time and space conformable fractional heat equations with conformable Fourier transform
Authors: Çenesiz Yücel , Kurt Ali 
Published: February 2016

Abstract
In this paper our aim is to find the solutions of time and space fractional heat differential equations by using new definition of fractional derivative called conformable fractional derivative. Also based on conformable fractional derivative definition conformable Fourier Transform is defined. Fourier sine and Fourier cosine transform definitions are given and space fractional heat equation is solved by conformable Fourier transform.
Analytical and numerical results of fractional differential-difference equations
Authors: Khan Najeeb Alam , Riaz Fatima 
Published: February 2016

Abstract
In this paper, we examine the fractional differential-difference equation (FDDE) by employing the proposed sensitivity approach (SA) and Adomian transformation method (ADTM). In SA the nonlinear differential-difference equation is converted to infinite linear equations which have a wide criterion to solve for the analytical solution. By ADTM, the FDDE is converted into ordinary differential-difference equation that can be solved. We test both the techniques through some test problems which are arising in nonlinear dynamical systems and found that ADTM is equivalently appropriate and simpler method to handle than SA.
Erweiterung Komplexer Netzwerke zur dynamischen Modellierung und Analyse von großen, verkoppelten Systemen
Authors: Geiger Andreas , Kroll Andreas 
Published: February 2016

Zusammenfassung
Komplexe Netzwerke stellen eine Möglichkeit dar, große, verkoppelte Systeme zu modellieren
und zu analysieren. Für die Analyse werden dabei statistische Maße verwendet, welche auf den
Modellgraphen, bestehend aus Knoten und Kanten, angewandt werden. Nachteil einer solchen
Modellierung und Analyse ist jedoch das Ausblenden des dynamisch bedingten Verhaltens eines
Systems, welches jedoch für die Regelungstechnik eine wesentliche Bedeutung besitzt. Der
Beitrag stellt einen Ansatz vor, Komplexe Netzwerke so zu erweitern, dass das
regelungstechnisch relevante, dynamische Verhalten analysiert werden kann. Bei der
Modellierung werden dabei Kantengewichte verwendet, welche das dynamische Verhalten auf eine
einfache Art und Weise beschreiben. Der Ansatz wird am Beispiel der qualitativen Analyse des
Inverse-Response-Verhaltens vorgestellt und an einer Fallstudie demonstriert.
Random forests in non-invasive sensorimotor rhythm brain-computer interfaces: a practical and convenient non-linear classifier
Authors: Steyrl David , Scherer Reinhold , Faller Josef , Müller-Putz Gernot R. 
Published: February 2016

Abstract
There is general agreement in the brain-computer interface (BCI) community that although non-linear classifiers can provide better results in some cases, linear classifiers are preferable. Particularly, as non-linear classifiers often involve a number of parameters that must be carefully chosen. However, new non-linear classifiers were developed over the last decade. One of them is the random forest (RF) classifier. Although popular in other fields of science, RFs are not common in BCI research. In this work, we address three open questions regarding RFs in sensorimotor rhythm (SMR) BCIs: parametrization, online applicability, and performance compared to regularized linear discriminant analysis (LDA). We found that the performance of RF is constant over a large range of parameter values. We demonstrate – for the first time – that RFs are applicable online in SMR-BCIs. Further, we show in an offline BCI simulation that RFs statistically significantly outperform regularized LDA by about 3%. These results confirm that RFs are practical and convenient non-linear classifiers for SMR-BCIs. Taking into account further properties of RFs, such as independence from feature distributions, maximum margin behavior, multiclass and advanced data mining capabilities, we argue that RFs should be taken into consideration for future BCIs.
Assessing the eligibility of a non-invasive continuous blood pressure measurement technique for application during total intravenous anaesthesia
Authors: Huhle Robert , Siegert Joachim , Wonka Fred , Schindler Christoph , de Abreu Marcelo Gama , Koch Thea , Morgenstern Ute , Theilen Herman 
Published: February 2016

Abstract
Objective: To assess the eligibility for replacement of invasive blood pressure as measured “within” the arterial vessel (IBP) with non-invasive continuous arterial blood pressure (cNIP) monitoring during total intravenous anaesthesia (TIVA), the ability of cNiP to track fast blood pressure changes needs to be quantified. A new method of statistical data analysis is developed for this purpose.
Methods: In a pilot study on patients undergoing neurosurgical anaesthesia, mean arterial pressure MAPIBP measured with IBP was compared to MAPCNP measured by the CNAP Monitor 500 in ten patients (age: 63±13 a). Correlation analysis of changes of device differences ΔeMAP=ΔMAPCNP-ΔMAPIBP with changes of MAPIBP (ΔMAPIBP) during intervals of vasoactivity was conducted. An innovative technique, of linear trend analysis (LTA) applied to two signals, is described to perform this analysis without a priori knowledge of intervals of vasoactivity.
Results: Analysis of ΔeMAP during vasoactivity revealed that ΔMAPCNP systematically underestimated ΔMAPIBP by 37%. This was confirmed in the complete data set using LTA technique showing a systematic, yet patient specific, underestimation in tracking ΔMAPIBP (16…120%).
Conclusion: The proposed LTA technique is able to detect systematic errors in tracking short-term blood pressure changes otherwise masked by established analysis. LTA may thus be a useful tool to assess the eligibility of cNIP to replace IBP during TIVA.
Comparative analysis of prostate cancer specific biomarkers PCA3 and ERG in whole urine, urinary sediments and exosomes
Authors: Hendriks Rianne J. , Dijkstra Siebren , Jannink Sander A. , Steffens Martijn G. , van Oort Inge M. , Mulders Peter F.A. , Schalken Jack A. 
Published: February 2016

Abstract
Background: PCA3 and ERG are mRNA-based prostate cancer (PCa) specific biomarkers that can be detected in urine. However, urine is a complex substrate that can be separated in several fractions. In this study we compared the levels of PCa-specific biomarkers (PCA3 and ERG) and KLK3 as prostate-specific reference gene in three urine substrates–whole urine, urinary sediment (cell pellet) and exosomes–and evaluated the influence of performing a digital rectal examination (DRE) prior to urine sampling.
Methods: First-voided urine samples were prospectively obtained before and after DRE from 29 men undergoing prostate biopsies. The urine was separated in whole urine, cell pellet and exosomes and the biomarker levels were measured with RT-qPCR.
Results: PCa was identified in 52% (15/29) of men. In several samples the mRNA levels were below the analytical limit of detection (BDL). The biomarker levels were highest in whole urine and significantly higher after DRE in all substrates. In PCa patients higher levels of PCA3 and ERG were found in all urine substrates after DRE compared to non-PCa patients.
Conclusions: This is the first study in which urinary PCa-specific biomarker levels were compared directly in three separate urine fractions. These results suggest that whole urine could be the urine substrate of choice for PCa-diagnostics based on analytical sensitivity, which is reflected directly in the high informative rate. Moreover, the significant positive effect of performing a DRE prior to urine sampling is confirmed. These findings could be of influence in the development of PCa-diagnostic urine tests.
An International Standard for holotranscobalamin (holoTC): international collaborative study to assign a holoTC value to the International Standard for vitamin B12 and serum folate
Authors: Thorpe Susan J. , Rigsby Peter , Roberts Graham , Lee Anne , Hamilton Malcolm , Craig David 
Published: February 2016

AbstractBackground:Investigation of possible B12 and folate deficiencies requires measurement of these vitamins in serum. There is evidence that holotranscobalamin (holoTC), the active portion of B12 available to cells, is a more specific marker of early B12 deficiency than total B12. The availability of immunoassays for holoTC prompted an international collaborative study to assign a holoTC value to the World Health Organization (WHO) 1st International Standard (IS) for vitamin B12 and serum folate, 03/178.Methods:The IS, 03/178, and three serum samples with different holoTC levels were assayed by 12 laboratories in eight countries using manual and automated immunoassays for holoTC; one laboratory additionally performed an in-house assay. Fourteen sets of data were analysed.Results:Overall, the IS, 03/178, and the three serum samples demonstrated assay linearity and parallelism. An overall geometric mean (GM) holoTC value of 106.8 pmol/L was obtained for 03/178, with an inter-laboratory geometric coefficient of variation (GCV) of 10.5%. There was a reduction in inter-laboratory variability when the holoTC levels in the serum samples were determined relative to the IS with an assigned holoTC value rather than to the assays’ calibration. Accelerated degradation studies showed that 03/178 was sufficiently stable to serve as an IS for holoTC.Conclusions:The WHO Expert Committee on Biological Standardization endorsed the proposal to assign a holoTC value of 107 pmol/L to 03/178, corresponding to 0.107 pmol per ampoule, for use as the 1st IS for vitamin B12, serum folate, and holoTC.
A practical approach to non-spectral interferences elimination in inductively coupled plasma optical emission spectrometry
Authors: Krejčová Anna , Černohorský Tomáš , Bendakovská Lenka 
Published: February 2016

AbstractMatrix effects and practical possibilities of reducing accompanying non-spectral interferences in inductively coupled plasma optical emission spectrometry (ICP-OES) were studied for microconcentric Micromist, concentric and V-groove nebulizers (VGN) coupled with two cyclonic spray chambers of different sizes. The effect of a wide scale of interferents and mixtures thereof in the concentration range of up to 2 mass % (Na, Ca, Ba, La, urea) or up to 20 vol. % (nitric acid) on the analysis of Cd, Cu, K, Mg, Mn, Pb and Zn was investigated in terms of their analytical recovery and Mg(II) 280.27 nm/Mg(I) 285.29 nm line intensity ratio. Recoveries of ionic lines were lower than those of atomic lines (37–102 %) depending on the matrix concentration. The Mg(II)/Mg(I) ratios were found to be 12–15 and they slightly decreased as the matrix load increased. Exceptional behavior of pure La matrix, steeply lowering the recoveries and Mg(II)/Mg(I) ratios was observed. A Micromist nebulizer coupled with a small inner volume spray chamber provided the highest recoveries (94–102 %), lowest matrix effects across the matrix loads and, compared to others, the least significant dependence without worsening of the analytical characteristics (recoveries, signal background ratios and the Mg(II)/Mg(I) ratios) across the studied matrices.
Pharmacogenetics and pharmacogenomics as tools in cancer therapy
Authors: Rodríguez-Vicente Ana E. , Lumbreras Eva , Hernández Jesus M. , Martín Miguel , Calles Antonio , Otín Carlos López , Algarra Salvador Martín , Páez David , Taron Miquel 
Published: February 2016

Abstract
Pharmacogenetics and pharmacogenomics (PGx) are rapidly growing fields that aim to elucidate the genetic basis for the interindividual differences in drug response. PGx approaches have been applied to many anticancer drugs in an effort to identify relevant inherited or acquired genetic variations that may predict patient response to chemotherapy and targeted therapies. In this article, we discuss the advances in the field of cancer pharmacogenetics and pharmacogenomics, driven by the recent technological advances and new revolutionary massive sequencing technologies and their application to elucidate the genetic bases for interindividual drug response and the development of biomarkers able to personalize drug treatments. Specifically, we present recent progress in breast cancer molecular classifiers, cell-free circulating DNA as a prognostic and predictive biomarker in cancer, patient-derived tumor xenograft models, chronic lymphocytic leukemia genomic landscape, and current pharmacogenetic advances in colorectal cancer. This review is based on the lectures presented by the speakers of the symposium “Pharmacogenetics and Pharmacogenomics as Tools in Cancer Therapy” from the VII Conference of the Spanish Pharmacogenetics and Pharmacogenomics Society (SEFF), held in Madrid (Spain) on April 21, 2015.
Effects of the introduction of pre-treated wastewater in a shallow lake reed stand
Authors: Dinka Mária , Kiss Anita , Magyar Norbert , Ágoston-Szabó Edit 
Published: February 2016

AbstractReed stands may be employed in the amelioration of water quality or even in the treatment of wastewater. In this study, the nutrient concentrations of (i) the above- and below-ground Common Reed (Phragmites australis) biomass, and (ii) surface and interstitial water were analyzed in a natural stand used in wastewater treatment. The reed stand was located in Hungarian part of Lake Fertő/Neusiedler See, by the shore near Fertőrákos Bay. The nitrate, phosphate and dissolved organic nitrogen concentrations of surface water were found to be higher on the inlet side of the reed stand compared to the outlet. The N and P concentrations in the above-ground biomass and P concentrations in the below-ground biomass increased after the introduction of pre-treated wastewater. The inter-annual differences in the characteristics of sediment interstitial water and in the nutrient content of reed tissues were assessed using statistical methods. The samples taken before and after the introduction of the pre-treated wastewater in the parcel formed different clusters. The results of the study provide further evidence that the nutrient retention capacity of natural stands ofP. australismay be employed in the treatment of wastewater while protecting and preserving the valuable natural assets of the lake.
The Czech language of Jews in Přemyslid Bohemia of the eleventh to fourteenth century
Authors: Dittmann Robert 
Published: February 2016

AbstractThe contribution discusses aspects of the language of Czech Jewry residing in Prague during the Přemyslid reign. The author shows that their vernacular was basically identical with contemporary Prague Czech. The corpus of Canaanite, i.e., Slavic, especially Old Czech glosses in Hebrew script is remarkable for its orthography, phonology, lexicology and grammar. The author discusses the linguistic situation with respect to multilingualism, Slavic interpretation of the glosses, their functions and features and several sociolinguistic aspects such as archaisation, place names in communication and name giving.
Romani in the Czech sociolinguistic space
Authors: Eckert Eva 
Published: February 2016

AbstractThe study was prompted by the question of whether Romani, the language of the dominant and traditional minority, is acceptable as a language in the profile of a bilingual living in the Czech Republic. It outlines the situation of Romani in the Czech sociolinguistic space and argues for its rehabilitation so that it could be studied, taught, maintained in a community, and used to represent its speakers. This is necessary in order to raise its prestige, positively affect its speakers’ identity and in turn stimulate an attitudinal shift and social change. Obstacles to Romani rehabilitation are related to it being framed by Czech culture, which is driven by a standard language ideology rendering Romani a stigmatized language. Romani speakers distance themselves from Romani dialects and the ethnolect as expressions of social and economic disadvantage. Recent research has established that Romani has shown signs of language shift.
Modeling of battery life optimal charging strategies based on empirical mobility data
Authors: Schoch Jennifer 
Published: February 2016

Abstract
Battery electric vehicles (EVs) have a more limited range and longer
refueling, i. e. charging times accompanied by battery aging as
conventional vehicles. Initial field studies about the behavior of EV
users also have identified the presence of range anxiety, which is
expressed by frequent recharging. Battery aging is a non-linear
process with numerous interactions, but generally pronounced around
higher state of charge (SoC) levels. Therefore, range anxiety
potentially provokes elevated battery degradation due to the resulting
operating conditions. Based on 2966 empirical driving
profiles, I analyze different user groups as well as the effect of
different charging strategies in a simulation-based analysis. In
comparison to charging strategies, for instance as fast as possible
(AFAP) charging or critical SoC charging (such that the next trip is
feasible), I calculate the degradation optimal charging pattern based
on a realistic battery cell aging model. The model is derived from
accelerated aging tests of Li-NMC 18650 cells, but can be adjusted to
individual degradation parameters, in order to account for different
battery chemistries. Preliminary results show that degradation can be
reduced by up to 46% by the optimized charging strategy as
compared to AFAP or critical SoC charging.