bluuebunny/crossref_metadata_2025_split huggingface dataset:
JA but not JA‐Ile is the cell‐nonautonomous signal activating JA mediated systemic defenses to herbivory in Nicotiana attenuata
Authors: Bozorov Tohir A. , Dinh Son Truong , Baldwin Ian T. 
Published: April 2017

AbstractThe whole‐plant activation of defense responses to wounding and herbivory requires systemic signaling in which jasmonates (JAs) play a pivotal role. To examine the nature of the slower cell‐nonautonomous as compared to the rapid cell‐autonomous signal in mediating systemic defenses in Nicotiana attenuata, reciprocal stem grafting‐experiments were used with plants silenced for the JA biosynthetic gene ALLENE OXIDE CYCLASE (irAOC) or plants transformed to create JA sinks by ectopically expressing Arabidopsis JA‐O‐methyltransferase (ovJMT). JA‐impaired irAOC plants were defective in the cell‐nonautonomous signaling pathway but not in JA transport. Conversely, ovJMT plants abrogated the production of a graft‐transmissible JA signal. Both genotypes displayed unaltered cell‐autonomous signaling. Defense responses (17‐hydroxygeranyllinalool diterpene glycosides, nicotine, and proteinase inhibitors) and metabolite profiles were differently induced in irAOC and ovJMT scions in response to graft‐transmissible signals from elicited wild type stocks. The performance of Manduca sexta larvae on the scions of different graft combinations was consistent with the patterns of systemic defense metabolite elicitations. Taken together, we conclude that JA and possibly MeJA, but not JA‐Ile, either directly functions as a long‐distance transmissible signal or indirectly interacts with long distance signal(s) to activate systemic defense responses.
Career Cartography: From Stories to Science and Scholarship
Authors: Wilson Deleise S. , Rosemberg Marie‐Anne S. , Visovatti Moira , Munro‐Kramer Michelle L. , Feetham Suzanne 
Published: April 2017

AbstractPurposeTo present four case scenarios reflecting the process of research career development using career cartography.Organizing ConstructsCareer cartography is a novel approach that enables nurses, from all clinical and academic settings, to actively engage in a process that maximizes their clinical, teaching, research, and policy contributions that can improve patient outcomes and the health of the public.MethodsFour early‐career nurse researchers applied the career cartography framework to describe their iterative process of research career development. They report the development process of each of the components of career cartography, including destination statement, career map, and policy statement.ConclusionsDespite diverse research interests and career mapping approaches, common experiences emerged from the four nurse researchers. Common lessons learned throughout the career cartography process include: (a) have a supportive mentorship team, (b) start early and reflect regularly, (c) be brief and to the point, (d) keep it simple and avoid jargon, (e) be open to change, (f) make time, and (g) focus on the overall career destination.Clinical RelevanceThese four case scenarios support the need for nurse researchers to develop their individual career cartography. Regardless of their background, career cartography can help nurse researchers articulate their meaningful contributions to science, policy, and health of the public.
Comparison of the nephrotoxic effects of iodixanol versus iohexol in patients with chronic heart failure undergoing coronary angiography or angioplasty
Authors: Song Tao , Song Min , Ge Zhiming , Li Yong , Shi Peimiao , Sun Menghan 
Published: April 2017

ObjectivesThe aim of this clinical trial is to compare iodixanol with iohexol for the incidence of contrast‐induced nephropathy in patients with chronic heart failure with reduced ejection fraction who are currently undergoing coronary angiography or angioplasty.MethodsThe clinical trial included 220 consecutive patients with chronic heart failure with reduced ejection fraction undergoing coronary angiography or angioplasty. Study participants were administered either iodixanol (n = 110) or iohexol (n = 110). The primary study endpoint was the incidence of contrast‐induced nephropathy within 72 h after the procedure. The secondary endpoints were to determine the peak increase in serum creatinine levels and Cystatin C, and the peak decrease in estimated glomerular filtration rate at 72 h post‐contrast medium.ResultsBaseline demographic and clinical characteristics of the patients were similar between the two groups. Our study showed that the overall incidence of contrast induced nephropathy in patients with chronic heart failure was 20.9%. The incidence of contrast induced nephropathy was significantly lower in iodixanol group than in iohexol group (29.1% vs 12.7%, P = 0.041). The peak increase in serum creatinine levels and the peak decrease in estimated glomerular filtration rate after the procedure were statistically significant between the two groups. Moreover, there was statistically significance in the peak increase of Cystatin C levels after the procedure.ConclusionsIn patients with chronic heart failure with reduced ejection fraction who are currently undergoing coronary angiography with or without percutaneous coronary intervention, the iso‐osmolar contrast iodixanol was associated with a lower incidence of contrast induced nephropathy than low‐osmolar contrast iohexol.
Comprehensive Review and Comparison of the Disinfection Techniques Currently Available in the Literature
Authors: Chidambaranathan Ahila S. , Balasubramanium Muthukumar 
Published: April 2017

AbstractThe goal of disinfection in the dental office is to prevent the spread of infection from one patient to another and maintain the safety of the dental care providers. Prevention of cross infection has significant effect on infection control. The standard procedure of rinsing impressions under tap water immediately after removal from the mouth eliminates microorganisms along with saliva and blood. A broad search on published literature was done using the keywords impression materials, disinfection method, and sterilization of dental impression from 1980 to 2016 in Medline, Google Scholar, the internet, and textbooks. This article critically analyzes the various published methods of dental impression disinfection in dentistry.
Therapeutic Efficacy of Twin‐Block and Fixed Oral Appliances in Patients with Obstructive Sleep Apnea Syndrome
Authors: Umemoto George , Toyoshima Hideo , Yamaguchi Yuji , Aoyagi Naoko , Yoshimura Chikara , Funakoshi Kouta 
Published: April 2017

AbstractPurposeTo compare the efficacy of twin‐block (i.e., allows mouth opening) and fixed (i.e., maintains mouth closure) mandibular advancement splints (MASs) for the treatment of obstructive sleep apnea–hypopnea syndrome (OSA).Materials and MethodsFrom 2011 to 2013, 23 patients with OSA in the twin‐block group, and from 2013 to 2015, 29 patients in the fixed MAS group were included. All patients underwent polysomnography before and after 3 months of treatment. The two sets of polysomnographic and cephalometric variables were compared.ResultsA significant difference (p < 0.001) was observed in the apnea–hypopnea index before and after MAS treatment in both groups (twin‐block group: 20.6 ± 11.5 vs. 14.7 ± 9.4; fixed group: 21.4 ± 15.2 vs. 11.2 ± 9.7). In the twin‐block group, 5 patients (21.7%) were complete responders, 9 (39.1%) were fair responders, and 9 (39.1%) were nonresponders; the corresponding figures for the fixed group were 14 (48.3%), 9 (31.0%), and 6 (20.7%) patients. A significant between‐group difference was observed in the distribution of responders (p = 0.046). The fixed group showed a significant improvement in the snoring index (p = 0.003), arousal index (p = 0.036), and desaturation rate (p = 0.012). Finally, the change in incisal overjet was larger in the fixed group than in the twin‐block group (p < 0.001).ConclusionsThese results suggest that fixed oral appliances are superior in treating OSA, based on their ability to prevent mouth opening and reduce incisal overjet.
High payload itraconazole-incorporated lipid nanoparticles with modulated release property for oral and parenteral administration
Authors: Zeb Alam , Qureshi Omer S , Kim Hoo-Seong , Kim Myung-Sic , Kang Jong-Ho , Park Jeong-Sook , Kim Jin-Ki 
Published: April 2017

Abstract

Objectives
The aim of this study was to develop high payload itraconazole-incorporated lipid nanoparticles (HINP) with modulated release property using a binary mixture core of solid and liquid lipid for oral and parenteral administration.

Methods
High payload itraconazole-incorporated lipid nanoparticles were prepared by hot high-pressure homogenization method using tristearin (TS) as a solid lipid, triolein (TO) as a liquid lipid and egg phosphatidylcholine/Tween 80/DSPE-PEG2000 as a surfactants mixture. To investigate the effects of liquid lipid in lipid core on itraconazole (ITZ) dissolution and release, TS/TO ratio was varied as 100/0, 90/10 and 80/20 (mg/mg).

Key findings
All HINP formulations showed particle size around 300 nm and polydispersity index below 0.3. The incorporation efficiencies of HINP formulations were above 80%, and more than 40 mg of ITZ was incorporated into each HINP formulation. In-vitro dissolution and release rate of ITZ from HINP increased as the amount of TO in lipid core increased. Compared with commercial formulations of ITZ, the pharmacokinetics of ITZ was improved after oral and parenteral administration of HINP formulations containing 0% or 10% of TO in lipid core.

Conclusion
High payload itraconazole-incorporated lipid nanoparticles with a binary mixture lipid core have a great potential for the development of controlled release formulation of ITZ.
Drivers of intraspecific trait variation of grass and forb species in German meadows and pastures
Authors: Herz Katharina , Dietz Sophie , Haider Sylvia , Jandt Ute , Scheel Dierk , Bruelheide Helge 
Published: April 2017

AbstractQuestionsTo what extent is trait variation in grasses and forbs driven by land‐use intensity, climate, soil conditions and plant diversity of the local neighbourhood? Do grass and forb species differ in the degree of intraspecific trait variation?LocationManaged grasslands in three regions of Germany.MethodsUsing a phytometer approach, we raised 20 common European grassland species (ten forbs and ten grasses) and planted them into 54 plots of different land‐use types (pasture, meadow, mown pasture). After 1 yr in the field, we measured above‐ and below‐ground plant functional traits. Linear mixed effects models (LMEM) were used to identify the most powerful predictors for every trait. Variation partitioning was applied to assess the amount of inter‐ and intraspecific trait variation in grasses and forbs explained by environmental conditions (land‐use intensity, climate and soil conditions) and plant species diversity of the local neighbourhood.ResultsFor 12 out of the 14 traits studied, either land‐use intensity or local neighbourhood diversity were predictors in the best LMEM. Land‐use intensity had considerably stronger effects than neighbourhood diversity. Root dry matter content and root phosphorus concentration of forbs were more affected by land‐use intensity than those of grasses. For almost all traits, intraspecific trait variation of grasses was much higher than that of forbs, while traits of forbs varied more among species. Overall, inter‐ and intraspecific variation was of the same magnitude.ConclusionThe similar magnitude of intra‐ and interspecific trait variation suggests that both sources should be considered in grassland studies at a scale similar to that of our study. The high amount of intraspecific trait variation that was explained by environmental factors and local neighbourhood diversity clearly demonstrates the high potential of species to adjust to local conditions, which would be ignored when only considering species mean trait values.
Fair bilateral pricing under funding costs and exogenous collateralization
Authors: Nie Tianyang , Rutkowski Marek 
Published: April 2017

AbstractBielecki and Rutkowski introduced and studied a generic nonlinear market model, which includes several risky assets, multiple funding accounts, and margin accounts. In this paper, we examine the pricing and hedging of contract from the perspective of both the hedger and the counterparty with arbitrary initial endowments. We derive inequalities for unilateral prices and we study the range of fair bilateral prices. We also examine the positive homogeneity and monotonicity of unilateral prices with respect to the initial endowments. Our study hinges on results from Nie and Rutkowski for backward stochastic differential equations (BSDEs) driven by continuous martingales, but we also derive the pricing partial differential equations (PDEs) for path‐independent contingent claims of a European style in a Markovian framework.
Arbitrage‐free XVA
Authors: Bichuch Maxim , Capponi Agostino , Sturm Stephan 
Published: April 2017

AbstractWe develop a framework for computing the total valuation adjustment (XVA) of a European claim accounting for funding costs, counterparty credit risk, and collateralization. Based on no‐arbitrage arguments, we derive backward stochastic differential equations associated with the replicating portfolios of long and short positions in the claim. This leads to the definition of buyer's and seller's XVA, which in turn identify a no‐arbitrage interval. In the case that borrowing and lending rates coincide, we provide a fully explicit expression for the unique XVA, expressed as a percentage of the price of the traded claim, and for the corresponding replication strategies. In the general case of asymmetric funding, repo, and collateral rates, we study the semilinear partial differential equations characterizing buyer's and seller's XVA and show the existence of a unique classical solution to it. To illustrate our results, we conduct a numerical study demonstrating how funding costs, repo rates, and counterparty risk contribute to determine the total valuation adjustment.
Park Forest (L5) and the asteroidal source of shocked L chondrites
Authors: Meier Matthias M. M. , Welten Kees C. , Riebe My E. I. , Caffee Marc W. , Gritsevich Maria , Maden Colin , Busemann Henner 
Published: April 2017

AbstractThe Park Forest (L5) meteorite fell in a suburb of Chicago, Illinois (USA) on March 26, 2003. It is one of the currently 25 meteorites for which photographic documentation of the fireball enabled the reconstruction of the meteoroid orbit. The combination of orbits with pre‐atmospheric sizes, cosmic‐ray exposure (CRE), and radiogenic gas retention ages (“cosmic histories”) is significant because they can be used to constrain the meteoroid's “birth region,” and test models of meteoroid delivery. Using He, Ne, Ar, 10Be, and 26Al, as well as a dynamical model, we show that the Park Forest meteoroid had a pre‐atmospheric size close to 180 g cm−2, 0–40% porosity, and a pre‐atmospheric mass range of ~2–6 tons. It has a CRE age of 14 ± 2 Ma, and (U, Th)‐He and K‐Ar ages of 430 ± 90 and 490 ± 70 Ma, respectively. Of the meteorites with photographic orbits, Park Forest is the second (after Novato) that was shocked during the L chondrite parent body (LCPB) break‐up event approximately 470 Ma ago. The suggested association of this event with the formation of the Gefion family of asteroids has recently been challenged and we suggest the Ino family as a potential alternative source for the shocked L chondrites. The location of the LCPB break‐up event close to the 5:2 resonance also allows us to put some constraints on the possible orbital migration paths of the Park Forest meteoroid.
Vitamin D status of White pregnant women and infants at birth and 4 months in North West England: A cohort study
Authors: Emmerson Anthoney J. B. , Dockery Karen Elizabeth , Mughal M. Z. , Roberts Stephen A. , Tower Clare Louise , Berry Jacqueline L. 
Published: April 2017

AbstractThe prevalence of vitamin D deficiency in pregnant white‐skinned women (WSW) and their infants has not been investigated at northern latitudes in a developed county. A 2‐year observational cohort study was undertaken in the North West of England to determine 25‐hydroxyvitamin D (25OHD) levels in WSW and their infants during pregnancy and 4 months postdelivery and to explore factors associated with these levels. Nutritional and lifestyle questionnaires were completed and 25OHD levels measured at 28 weeks and 4 months postdelivery.Twenty‐seven percent and 7% of WSW had insufficient and deficient levels of 25OHD during pregnancy and 48% and 11% four months postdelivery. WSW with Fitzpatrick skin‐type I (FST I) have significantly lower 25OHD than other skin types after controlling for time spent outside and vitamin D intake. Twenty‐four percent and 13% of infants had insufficient and deficient 25OHD levels at 4 months. Unsupplemented breast‐fed infants have the highest level of insufficiency (67%) compared with formula‐fed infants (2%). Factors associated with infant serum 25OHD levels at 4 months included breast feeding, supplementation, and time outside. WSW have a high prevalence of insufficiency and deficiency during pregnancy which doubles 4 months after birth. Breast‐fed infants of WSW are rarely considered at risk of vitamin D insufficiency but have high rates compared with formula‐fed infants. This is the first study to show the finding that FST I WSW have significantly lower levels of 25OHD than those with FST II–IV (difference adjusted for diet and time outside 14 (95%CI 7–21) nmol/L).
Small serine recombination systems ParA‐MRS and CinH‐RS2 perform precise excision of plastid DNA
Authors: Shao Min , Blechl Ann , Thomson James G. 
Published: April 2017

AbstractSelectable marker genes (SMGs) are necessary for selection of transgenic plants. However, once stable transformants have been identified, the marker gene is no longer needed. In this study, we demonstrate the use of the small serine recombination systems, ParA‐MRS and CinH‐RS2, to precisely excise a marker gene from the plastid genome of tobacco. Transplastomic plants transformed with the pTCH‐MRS and pTCH‐RS2 vectors, containing the visual reporter gene DsRed flanked by directly oriented MRS and RS2 recognition sites, respectively, were crossed with nuclear‐genome transformed tobacco plants expressing plastid‐targeted ParA and CinH recombinases, respectively. One hundred per cent of both types of F1 hybrids exhibited excision of the DsRed marker gene. PCR and Southern blot analyses of DNA from F2 plants showed that approximately 30% (CinH‐RS2) or 40% (ParA‐MRS) had lost the recombinase genes by segregation. The postexcision transformed plastid genomes were stable and the excision events heritable. The ParA‐MRS and CinH‐RS2 recombination systems will be useful tools for site‐specific manipulation of the plastid genome and for generating marker‐free plants, an essential step for reuse of SMG and for addressing concerns about the presence of antibiotic resistance genes in transgenic plants.
Cloning and plant‐based production of antibody MC10E7 for a lateral flow immunoassay to detect [4‐arginine]microcystin in freshwater
Authors: Melnik Stanislav , Neumann Anna‐Cathrine , Karongo Ryan , Dirndorfer Sebastian , Stübler Martin , Ibl Verena , Niessner Reinhard , Knopp Dietmar , Stoger Eva 
Published: April 2017

SummaryAntibody MC10E7 is one of a small number of monoclonal antibodies that bind specifically to [Arg4]‐microcystins, and it can be used to survey natural water sources and food samples for algal toxin contamination. However, the development of sensitive immunoassays in different test formats, particularly user‐friendly tests for on‐site analysis, requires a sensitive but also cost‐effective antibody. The original version of MC10E7 was derived from a murine hybridoma, but we determined the sequence of the variable regions using the peptide mass‐assisted cloning strategy and expressed a scFv (single‐chain variable fragment) format of this antibody in yeast and a chimeric full‐size version in leaves of Nicotiana tabacum and Nicotiana benthamiana to facilitate inexpensive and scalable production. The specific antigen‐binding activity of the purified antibody was verified by surface plasmon resonance spectroscopy and ELISA, confirming the same binding specificity as its hybridoma‐derived counterpart. The plant‐derived antibody was used to design a lateral flow immunoassay (dipstick) for the sensitive detection of [Arg4]‐microcystins at concentrations of 100–300 ng/L in freshwater samples collected at different sites. Plant‐based production will likely reduce the cost of the antibody, currently the most expensive component of the dipstick immunoassay, and will allow the development of further antibody‐based analytical devices and water purification adsorbents for the efficient removal of toxic contaminants.
By our bootstraps: Comparing methods for measuring auditory 40 Hz steady‐state neural activity
Authors: Edgar J. Christopher , Fisk IV Charles L. , Chen Yu‐Han , Stone‐Howell Breannan , Hunter Michael A. , Huang Mingxiong , Bustillo Juan R. , Cañive José M. , Miller Gregory A. 
Published: April 2017

AbstractAlthough the 40 Hz auditory steady‐state response (ASSR) is of clinical interest, the construct validity of EEG and MEG measures of 40 Hz ASSR cortical microcircuits is unclear. This study evaluated several MEG and EEG metrics by leveraging findings of (a) an association between the 40 Hz ASSR and age in the left but not right hemisphere, and (b) right‐ > left‐hemisphere differences in the strength of the 40 Hz ASSR. The contention is that, if an analysis method does not demonstrate a left 40 Hz ASSR and age relationship or hemisphere differences, then the obtained measures likely have low validity. Fifty‐three adults were presented 500 Hz stimuli modulated at 40 Hz while MEG and EEG were collected. ASSR activity was examined as a function of phase similarity (intertrial coherence) and percent change from baseline (total power). A variety of head models (spherical and realistic) and a variety of dipole source modeling strategies (dipole source localization and dipoles fixed to Heschl's gyri) were compared. Several sensor analysis strategies were also tested. EEG sensor measures failed to detect left 40 Hz ASSR and age associations or hemisphere differences. A comparison of MEG and EEG head‐source models showed similarity in the 40 Hz ASSR measures and in estimating age and left 40 Hz ASSR associations, indicating good construct validity across models. Given a goal of measuring the 40 Hz ASSR cortical microcircuits, a source‐modeling approach was shown to be superior in measuring this construct versus methods that rely on EEG sensor measures.