bluuebunny/crossref_metadata_2025_split huggingface dataset:
Negative emotions in art reception: Refining theoretical assumptions and adding variables to the Distancing-Embracing model
Authors: Menninghaus Winfried , Wagner Valentin , Hanich Julian , Wassiliwizky Eugen , Jacobsen Thomas , Koelsch Stefan 
Published: November 2017

AbstractWhile covering all commentaries, our response specifically focuses on the following issues: How can the hypothesis of emotional distancing (qua art framing) be compatible with stipulating high levels of felt negative emotions in art reception? Which concept of altogether pleasurable mixed emotions does our model involve? Can mechanisms of predictive coding, social sharing, and immersion enhance the power of our model?
Repair of interrupted aortic arch using autologous pulmonary artery tissue
Authors: Lee Cheul , Lee Jae Young , Lee Eun-Jung 
Published: November 2017

AbstractThere are several options for the repair of interrupted aortic arch. Direct anastomosis may cause several problems including anastomotic site stenosis, left main bronchus compression, and acute-angled aortic arch. Interposition of a prosthetic graft has no growth potential. We present a case of 34-month-old child with interrupted aortic arch, which was repaired using a pulmonary autograft tube.
Working Women and Monstrous Mothers: Kant, Marx, and the Valuation of Domestic Labour
Authors: Pascoe Jordan 
Published: November 2017

AbstractIn this article, I compare Kant’s and Marx’s analysis of women and domestic labour in their mature political works, and argue that Kant offers more analytic tools for understanding the social and economic role of domestic labour than does Marx. While domestic labour becomes visible to Marx only as it is outsourced, Kant develops a clear account of the specific rules governing domestic labour in the emerging bourgeois household. Because of his commitment to the domestic realm as a core feature of the just state, however, much of Kant’s account of domestic labour should be challenged by contemporary Kantian feminists.
From Revelation to Revolution: The Critique of Religion in Kant and Marx
Authors: Ypi Lea 
Published: November 2017

AbstractThis article examines Kant’s and Marx’s analysis of religion in its relation to human emancipation. It highlights some important affinities in their accounts of human nature and their critique of religious authority including: the emphasis on freedom as distinguishing human beings from other species, the relation between moral and political progress, the critique of revealed religion, the role of political community and the importance of ethical community to achieve moral emancipation.
Genome-wide association study of subcortical brain volume in PTSD cases and trauma-exposed controls
Authors: Morey Rajendra A. , Davis Sarah L. , Garrett Melanie E. , Haswell Courtney C. ,   Mid-Atlantic MIRECC Workgroup, Marx Christine E. , Beckham Jean C. , McCarthy Gregory , Hauser Michael A. , Ashley-Koch Allison E. 
Published: November 2017

AbstractDepending on the traumatic event, a significant fraction of trauma survivors subsequently develop PTSD. The additional variability in PTSD risk is expected to arise from genetic susceptibility. Unfortunately, several genome-wide association studies (GWAS) have failed to identify a consistent genetic marker for PTSD. The heritability of intermediate phenotypes such as regional brain volumes is often 80% or higher. We conducted a GWAS of subcortical brain volumes in a sample of recent military veteran trauma survivors (n = 157), grouped into PTSD (n = 66) and non-PTSD controls (n = 91). Covariates included PTSD diagnosis, sex, intracranial volume, ancestry, childhood trauma, SNP×PTSD diagnosis, and SNP×childhood trauma. We identified several genetic markers in high linkage disequilibrium (LD) with rs9373240 (p = 2.0 × 10−7, FDR q = 0.0375) that were associated with caudate volume. We also observed a significant interaction between rs9373240 and childhood trauma (p-values = 0.0007–0.002), whereby increased trauma exposure produced a stronger association between SNPs and increased caudate volume. We identified several SNPs in high LD with rs34043524, which is downstream of the TRAM1L1 gene that were associated with right lateral ventricular volume (p = 1.73 × 10−7; FDR q = 0.032) and were also associated with lifetime alcohol abuse or dependence (p = 2.49 × 10−7; FDR q = 0.0375). Finally, we identified several SNPs in high LD with rs13140180 (p = 2.58 × 10−7; FDR q = .0016), an intergenic region on chromosome 4, and several SNPs in the TMPRSS15 associated with right nucleus accumbens volume (p = 2.58 × 10−7; FDR q = 0.017). Both TRAM1L1 and TMPRSS15 have been previously implicated in neuronal function. Key results survived genome-wide multiple-testing correction in our sample. Leveraging neuroimaging phenotypes may offer a shortcut, relative to clinical phenotypes, in mapping the genetic architecture and neurobiological pathways of PTSD.
3D genome of multiple myeloma reveals spatial genome disorganization associated with copy number variations
Authors: Wu Pengze , Li Tingting , Li Ruifeng , Jia Lumeng , Zhu Ping , Liu Yifang , Chen Qing , Tang Daiwei , Yu Yuezhou , Li Cheng 
Published: November 2017

AbstractThe Hi-C method is widely used to study the functional roles of the three-dimensional (3D) architecture of genomes. Here, we integrate Hi-C, whole-genome sequencing (WGS) and RNA-seq to study the 3D genome architecture of multiple myeloma (MM) and how it associates with genomic variation and gene expression. Our results show that Hi-C interaction matrices are biased by copy number variations (CNVs) and can be used to detect CNVs. Also, combining Hi-C and WGS data can improve the detection of translocations. We find that CNV breakpoints significantly overlap with topologically associating domain (TAD) boundaries. Compared to normal B cells, the numbers of TADs increases by 25% in MM, the average size of TADs is smaller, and about 20% of genomic regions switch their chromatin A/B compartment types. In summary, we report a 3D genome interaction map of aneuploid MM cells and reveal the relationship among CNVs, translocations, 3D genome reorganization, and gene expression regulation.
Altered expression of metabolites and proteins in wild and caged fish exposed to wastewater effluents in situ
Authors: Simmons D. B. D. , Miller J. , Clarence S. , McCallum E. S. , Balshine S. , Chandramouli B. , Cosgrove J. , Sherry J. P. 
Published: November 2017

AbstractPopulation growth has led to increased global discharges of wastewater. Contaminants that are not fully removed during wastewater treatment, such as pharmaceuticals and personal care products (PPCPs), may negatively affect aquatic ecosystems. PPCPs can bioaccumulate causing adverse health effects and behavioural changes in exposed fish. To assess the impact of PPCPs on wild fish, and to assess whether caged fish could be used as a surrogate for resident wild fish in future monitoring, we caged goldfish in a marsh affected by discharges of wastewater effluents (Cootes Paradise, Lake Ontario, Canada). We collected plasma from resident wild goldfish, and from goldfish that we caged in the marsh for three weeks. We analyzed the plasma proteome and metabolome of both wild and caged fish. We also compared proteomic and metabolic responses in caged and wild fish from the marsh to fish caged at a reference site (Jordan Harbour Conservation Area). We identified significant changes in expression of over 250 molecules that were related to liver necrosis, accumulation and synthesis of lipids, synthesis of cyclic AMP, and the quantity of intracellular calcium in fish from the wastewater affected marsh. Our results suggest that PPCPs could be affecting the health of wild fish populations.
Elucidating the in vivo interactome of HIV-1 RNA by hybridization capture and mass spectrometry
Authors: Knoener Rachel A. , Becker Jordan T. , Scalf Mark , Sherer Nathan M. , Smith Lloyd M. 
Published: November 2017

AbstractHIV-1 replication requires myriad interactions between cellular proteins and the viral unspliced RNA. These interactions are important in archetypal RNA processes such as transcription and translation as well as for more specialized functions including alternative splicing and packaging of unspliced genomic RNA into virions. We present here a hybridization capture strategy for purification of unspliced full-length HIV RNA-protein complexes preserved in vivo by formaldehyde crosslinking, and coupled with mass spectrometry to identify HIV RNA-protein interactors in HIV-1 infected cells. One hundred eighty-nine proteins were identified to interact with unspliced HIV RNA including Rev and Gag/Gag-Pol, 24 host proteins previously shown to bind segments of HIV RNA, and over 90 proteins previously shown to impact HIV replication. Further analysis using siRNA knockdown techniques against several of these proteins revealed significant changes to HIV expression. These results demonstrate the utility of the approach for the discovery of host proteins involved in HIV replication. Additionally, because this strategy only requires availability of 30 nucleotides of the HIV-RNA for hybridization with a capture oligonucleotide, it is readily applicable to any HIV system of interest regardless of cell type, HIV-1 virus strain, or experimental perturbation.
Connectivity motifs of inhibitory neurons in the mouse Auditory Cortex
Authors: Oviedo Hysell V. 
Published: November 2017

AbstractConnectivity determines the function of neural circuits and it is the gateway to behavioral output. The emergent properties of the Auditory Cortex (ACx) have been difficult to unravel partly due to our assumption that it is organized similarly to other sensory areas. But detailed investigations of its functional connectivity have begun to reveal significant differences from other cortical areas that perform different functions. Using Laser Scanning Photostimulation we previously discovered unique circuit features in the ACx. Specifically, we found that the functional asymmetry of the ACx (tonotopy and isofrequency axes) is reflected in the local circuitry of excitatory inputs to Layer 3 pyramidal neurons. In the present study we extend the functional wiring diagram of the ACx with an investigation of the connectivity patterns of inhibitory subclasses. We compared excitatory input to parvalbumin (PV) and somatostatin (SOM)-expressing interneurons and found distinct circuit-motifs between and within these subpopulations. Moreover, these connectivity motifs emerged as intrinsic differences between the left and right ACx. Our results support a functional circuit based approach to understand the role of inhibitory neurons in auditory processing.
“Carboranyl-cysteine”—Synthesis, Structure and Self-Assembly Behavior of a Novel α-Amino Acid
Authors: He Tianyu , Misuraca Jennifer C. , Musah Rabi A. 
Published: November 2017

AbstractSubstitution of the thiol proton in cysteine withm-carborane furnished 2-amino-3-(1,7-dicarba-closo-dodecacarboranyl-1-thio)propanoic acid (3), a boron cluster amino acid that exhibits self-assembly to form micron-sized constructs. Field emission scanning electron microscopy revealed that ethanol solutions of3form floret-shaped constructs, while fibrillar architectures are formed in water. Furthermore, slow evaporation of methanol solutions of3produced crystals whose structure was revealed by X-ray crystallography. The crystal structure shows that the hydrogen bonding interactions between pairs of3result in the formation of bilayers of 174 Å in length. The orientation of the clusters is not random in the crystal structure, such that the side-by-side aligned polyhedra are offset by 158 degrees. The material was characterized by FT-IR, NMR, high resolution mass spectrometry and dynamic light scattering. Circular dichroism studies indicated that self-assembly occurs at concentrations as low as 0.01 μM. This represents the first demonstration of self-assembly of a carborane-based molecule in the absence of metals. The amino acid motif provides opportunities for the controlled synthesis of extended multimeric units with tunable properties and the potential for applications in biology, medicine and materials chemistry.
Mouse papillomavirus infection persists in mucosal tissues of an immunocompetent mouse strain and progresses to cancer
Authors: Cladel Nancy M. , Budgeon Lynn R. , Balogh Karla K. , Cooper Timothy K. , Brendle Sarah A. , Christensen Neil D. , Schell Todd D. , Hu Jiafen 
Published: November 2017

AbstractMouse papillomavirus has shown broad tissue tropism in nude mice. Previous studies have tested cutaneous infections in different immunocompromised and immunocompetent mouse strains. In the current study, we examined mucosal infection in several immunocompetent and immunocompromised mouse strains. Viral DNA was monitored periodically by Q-PCR of lavage samples. Immunohistochemistry and in situ hybridization were used to determine viral capsid protein and viral DNA respectively. All athymic nude mouse strains showed active infections at both cutaneous and mucosal sites. Interestingly, NOD/SCID mice, which have a deficiency in T, B, and NK cells, showed minimal disease at cutaneous sites but developed persistent infection at the mucosal sites including those of the anogenital region and the oral cavity. Three strains of immunocompetent mice supported mucosal infections. Infections of the lower genital tract in heterozygous (immunocompetent) mice of the NU/J strain progressed to high grade dysplasia and to carcinoma in situ. Anti-MmuPV1 neutralizing antibodies were detected in the sera of all immunocompetent animals. Our findings demonstrate that the mucosae may be the preferred sites for this virus in mice. The mouse model is expected to be a valuable model for the study of mucosal papillomavirus disease, progression, and host immune control.
Long-term drug modification to the surface of mesenchymal stem cells by the avidin-biotin complex method
Authors: Takayama Yukiya , Kusamori Kosuke , Hayashi Mika , Tanabe Noriko , Matsuura Satoru , Tsujimura Mari , Katsumi Hidemasa , Sakane Toshiyasu , Nishikawa Makiya , Yamamoto Akira 
Published: November 2017

AbstractMesenchymal stem cells (MSCs) have various functions, making a significant contribution to tissue repair. On the other hand, the viability and function of MSCs are not lasting after an in vivo transplant, and the therapeutic effects of MSCs are limited. Although various chemical modification methods have been applied to MSCs to improve their viability and function, most of conventional drug modification methods are short-term and unstable and cause cytotoxicity. In this study, we developed a method for long-term drug modification to C3H10T1/2 cells, murine mesenchymal stem cells, without any damage, using the avidin-biotin complex method (ABC method). The modification of NanoLuc luciferase (Nluc), a reporter protein, to C3H10T1/2 cells by the ABC method lasted for at least 14 days in vitro without major effects on the cellular characteristics (cell viability, cell proliferation, migration ability, and differentiation ability). Moreover, in vivo, the surface Nluc modification to C3H10T1/2 cells by the ABC method lasted for at least 7 days. Therefore, these results indicate that the ABC method may be useful for long-term surface modification of drugs and for effective MSC-based therapy.
Correlations between gut microbiota community structures of Tibetans and geography
Authors: Lan Daoliang , Ji Wenhui , Lin Baoshan , Chen Yabing , Huang Cai , Xiong Xianrong , Fu Mei , Mipam Tserang Donko , Ai Yi , Zeng Bo , Li Ying , Cai Zhixin , Zhu Jiangjiang , Zhang Dawei , Li Jian 
Published: November 2017

AbstractMicrobial communities of human gut directly influence health and bear adaptive potential to different geography environment and lifestyles. However, knowledge about the influences of altitude and geography on the gut microbiota of Tibetans is currently limited. In this study, fecal microbiota from 208 Tibetans across six different locations were analyzed by MiSeq sequencing; these locations included Gannan, Gangcha, Tianzhu, Hongyuan, Lhasa and Nagqu, with altitudes above sea level ranging from 2800 m to 4500 m across the Tibetan plateau. Significant differences were observed in microbial diversity and richness in different locations. At the phylum level, gut populations of Tibetans comprised Bacteroidetes (60.00%), Firmicutes (29.04%), Proteobacteria (5.40%), and Actinobacteria (3.85%) and were marked by a low ratio (0.48) of Firmicutes to Bacteroidetes. Analysis based on operational taxonomic unit level revealed that core microbiotas included Prevotella, Faecalibacterium, and Blautia, whereas Prevotella predominated all locations, except Gangcha. Four community state types were detected in all samples, and they mainly belong to Prevotella, Bacteroides, and Ruminococcaceae. Principal component analysis and related correspondence analysis results revealed that bacterial profiles in Tibetan guts varied significantly with increasing altitude, BMI, and age, and facultative anaerobes were rich in Tibetan guts. Gut microbiota may play important roles in regulating high-altitude and geographical adaptations.
A nonlinear approach to identify pathological change of thyroid nodules based on statistical analysis of ultrasound RF signals
Authors: Xu Huan , Liu Chunrui , Yang Ping , Tu Juan , Yang Bin , Zhang Dong 
Published: November 2017

AbstractIn order to reassure the majority of patients with benign nodules from unnecessary needle biopsy, there is an increasing clinical requirement to identify benign and malignant thyroid nodules during ultrasound diagnosis. A nonlinear approach based on statistical analysis of ultrasound radio-frequency (RF) signals was developed for differential diagnosing the thyroid nodules to improve the diagnostic accuracy. Data from 44 patients with solitary thyroid nodules were collected, following with the ultrasound-guided fine needle aspiration (FNA) as the ground truth. The relative P-value (rP-value) was estimated to quantify the pathophysiologic changes by comparing the region of interest (ROI) with the no pathological change part in the thyroid gland using only one frame of raw RF data. The malignant nodules were distinguished from benign ones with high accuracy and high credibility (sensitivity = 100%, specificity = 80%). Suspicious nodules (rP-value < 0.5) could be picked out for FNA with no additional instruments. This method shows promising in differentiating malignant from benign thyroid nodules, subsequently avoiding unnecessary biopsies.
Salmonella small RNA fragment Sal-1 facilitates bacterial survival in infected cells via suppressing iNOS induction in a microRNA manner
Authors: Zhao Chihao , Zhou Zhen , Zhang Tianfu , Liu Fenyong , Zhang Chen-Yu , Zen Ke , Gu Hongwei 
Published: November 2017

AbstractSalmonella can hijack host atypical miRNA processing machinery to cleave its small non-coding RNA into a ~22-nt RNA fragment, Sal-1, which facilitates Salmonella survival in the infected host. The mechanism through which Sal-1 promotes Salmonella survival, however, remains unknown. In the present study, we reported that Sal-1 targets cellular inducible nitric oxide synthase (iNOS) in a miRNA manner, leading to attenuation of host cell iNOS/NO-mediated anti-microbial capacity. First, depletion of Sal-1 in Salmonella-infected epithelial cells significantly increased the iNOS level but not the levels of various inflammatory cytokines. Bioinformatics analysis and mutagenesis strategies were consistent with the identification of mRNA of iNOS as a target of Sal-1 in both human and mice. Second, western blot and immunohistochemical analysis confirmed that Sal-1 suppressed iNOS expression in vitro and in vivo, thus reducing the production of NO. Finally, Sal-1 facilitating Salmonella survival through suppressing iNOS induction was confirmed in mouse model by expressing mutated iNOS that is not targeted by Sal-1 in mice colon. In conclusion, our study provides new insight into the pathogenic mechanism of intracellular bacteria to modulate host innate immune response.
Suitability of two distinct approaches for the high-throughput study of the post-embryonic effects of embryo-lethal mutations in Arabidopsis
Authors: Muñoz-Nortes Tamara , Candela Héctor , Micol José Luis 
Published: November 2017

AbstractSeveral hundred genes are required for embryonic and gametophytic development in the model plant Arabidopsis thaliana, as inferred from the lethality of their mutations. Despite many of these genes are expressed throughout the plant life cycle, the corresponding mutants arrest at early stages, preventing the study of their post-embryonic functions by conventional methods. Clonal analysis represents an effective solution to this problem by uncovering the effects of embryo-lethal mutations in sectors of mutant cells within an otherwise normal adult plant. In this pilot study, we have evaluated the suitability of two sector induction methods for the large-scale study of the post-embryonic effects of embryo-lethal (emb) mutations in Arabidopsis. In line with the interests of our laboratory, we selected 24 emb mutations that damage genes that are expressed in wild-type vegetative leaves but whose effects on leaf development remain unknown. For the induction of mutant sectors in adult plants, we followed one approach based on the X-ray irradiation of ‘cell autonomy’ (CAUT) lines, and another based on the site-specific excision of transgenes mediated by Cre recombinase. We conclude that both methods are time-consuming and difficult to scale up, being better suited for the study of emb mutations on a case-by-case basis.
Learning the value of information and reward over time when solving exploration-exploitation problems
Authors: Cogliati Dezza Irene , Yu Angela J. , Cleeremans Axel , Alexander William 
Published: November 2017

AbstractTo flexibly adapt to the demands of their environment, animals are constantly exposed to the conflict resulting from having to choose between predictably rewarding familiar options (exploitation) and risky novel options, the value of which essentially consists of obtaining new information about the space of possible rewards (exploration). Despite extensive research, the mechanisms that subtend the manner in which animals solve this exploitation-exploration dilemma are still poorly understood. Here, we investigate human decision-making in a gambling task in which the informational value of each trial and the reward potential were separately manipulated. To better characterize the mechanisms that underlined the observed behavioural choices, we introduce a computational model that augments the standard reward-based reinforcement learning formulation by associating a value to information. We find that both reward and information gained during learning influence the balance between exploitation and exploration, and that this influence was dependent on the reward context. Our results shed light on the mechanisms that underpin decision-making under uncertainty, and suggest new approaches for investigating the exploration-exploitation dilemma throughout the animal kingdom.