bluuebunny/crossref_metadata_2025_split huggingface dataset:
A path already travelled in domestic orders? From fragmentation to constitutionalisation in the global legal order
Authors: Murray Colin R.G. , O'Donoghue Aoife 
Published: April 2017

AbstractTheories of fragmentation and constitutionalisation have long been presented as antagonistic accounts of the global legal order. Fragmentation theorists posit a non-hierarchical order explained in terms of the relationships between general and specialised areas of international law. Constitutionalisation’s adherents, by contrast, identify the global legal order’s ongoing transformation from horizontal and consent-based roots towards a hierarchal structure grounded upon fundamental principles. The proliferation of international tribunals has long been recognised as a factor muddying the picture of constitutionalisation and pointing towards fragmentation within international law. We argue, however, that this proliferation enhances the global order’s potential for constitutionalisation. The current state of fragmentation within the uncodified global order is comparable to long periods when the UK’s uncodified constitution exhibited the hallmarks of fragmented development. We bridge these supposedly rival explanations for the development of legal orders by re-evaluating the role played by competing courts in the UK’s constitutionalisation process, reassessing developments familiar to common-law historians through the prism of fragmentation theory. The UK example indicates that fragmentation is not, of itself, an insurmountable obstacle to constitutionalisation within the global order and may even mark a stage within this process. We employ lessons derived from this comparison to evaluate current flashpoints in relations between international tribunals, including the European Court of Justice’s Opinion 2/13 which has for now stymied the EU’s efforts to accede to the European Convention on Human Rights.
H.L.A. Hart's secondary rules: what do ‘officials’ really think?
Authors: Howarth David , Stark Shona Wilson 
Published: April 2017

AbstractThe impact of H.L.A. Hart'sThe Concept of Lawon modern legal thinking is undisputed. But does it reflect the reality of the way British institutions work? InConcept,Hart argued, amongst other things, that one of two ‘minimum conditions necessary and sufficient for the existence of a legal system’ was that ‘its rules of recognition specifying the criteria of legal validity and its rules of change and adjudication must be effectively accepted as common public standards of official behaviour by its officials’. In this paper, we begin the process of testing that statement empirically. Specifically, we ask whether non-judicial UK officials have a uniform view of what the rules of recognition, change and adjudication are, and whether they uniformly take an internal point of view towards them (i.e. whether they accept the rules and do not merely obey them). By way of a pilot study, thirty non-judicial UK officials were interviewed. Those officials comprised currently serving and retired senior civil servants, senior military officials, chief constables and local authority chief executives. The findings of the pilot study are presented in this paper. They allow us to deduce that Hart's statement might well be an inaccurate and incomplete description of the modern British constitution, and to comment on the implications of that conclusion.
Inducement of apoptosis by cucurbitacin E, a tetracyclic triterpenes, through death receptor 5 in human cervical cancer cell lines
Authors: Cheng Ya-Min , Shen Ching-Ju , Chang Chi-Chang , Chou Cheng-Yang , Tsai Ching-Chou , Hsu Yi-Chiang 
Published: April 2017

AbstractCervical cancer is the most common malignancy in women, for which conization or hysterectomy are the main therapy. Curcubitacin E (Cu E) is a natural compound-based drug which from the Guadi (climbing stem of Cucumic melo L). Previously shown to be an anti-tumor as well as a potent chemopreventive agent against several types of tumors. The present study, investigated anti-proliferation and apoptosis induced by Cu E in cervical cancer cell lines (HeLa and Ca Ski). The results indicate that the cytotoxicity is associated with accumulation in apoptosis but not necrosis. Cu E produced apoptosis as well as the up-regulation the expression of death receptor 5 (DR5). In addition, the DR5 gene activation in apoptosis, both effects increased proportionally with the dose of Cu E; however, mitosis delay was also dependant on the amount of Cu E treatment in the cancer cells. These results indicate that Cu E may delay cancer cell growth by apoptosis via upregulation of DR5 gene expression.
A metallic molybdenum dioxide with high stability for surface enhanced Raman spectroscopy
Authors: Zhang Qiqi , Li Xinshi , Ma Qiang , Zhang Qing , Bai Hua , Yi Wencai , Liu Jingyao , Han Jing , Xi Guangcheng 
Published: April 2017

AbstractCompared with noble metals, semiconductors with surface plasmon resonance effect are another type of SERS substrate materials. The main obstacles so far are that the semiconducting materials are often unstable and easy to be further oxidized or decomposed by laser irradiating or contacting with corrosive substances. Here, we report that metallic MoO2 can be used as a SERS substrate to detect trace amounts of highly risk chemicals including bisphenol A (BPA), dichloropheno (DCP), pentachlorophenol (PCP) and so on. The minimum detectable concentration was 10−7 M and the maximum enhancement factor is up to 3.75 × 106. To the best of our knowledge, it may be the best among the metal oxides and even reaches or approaches to Au/Ag. The MoO2 shows an unexpected high oxidation resistance, which can even withstand 300 °C in air without further oxidation. The MoO2 material also can resist long etching of strong acid and alkali.
Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant
Authors: Nie Rongxin , Stark Steven , Symersky Jindrich , Kaplan Ronald S. , Lu Min 
Published: April 2017

AbstractIntegral membrane proteins of the divalent anion/Na+ symporter (DASS) family translocate dicarboxylate, tricarboxylate or sulphate across cell membranes, typically by utilizing the preexisting Na+ gradient. The molecular determinants for substrate recognition by DASS remain obscure, largely owing to the absence of any substrate-bound DASS structure. Here we present 2.8-Å resolution X-ray structures of VcINDY, a DASS from Vibrio cholerae that catalyses the co-transport of Na+ and succinate. These structures portray the Na+-bound VcINDY in complexes with succinate and citrate, elucidating the binding sites for substrate and two Na+ ions. Furthermore, we report the structures of a humanized variant of VcINDY in complexes with succinate and citrate, which predict how a human citrate-transporting DASS may interact with its bound substrate. Our findings provide insights into metabolite transport by DASS, establishing a molecular basis for future studies on the regulation of this transport process.
LPP is a Src substrate required for invadopodia formation and efficient breast cancer lung metastasis
Authors: Ngan Elaine , Stoletov Konstantin , Smith Harvey W. , Common Jessica , Muller William J. , Lewis John D. , Siegel Peter M. 
Published: April 2017

AbstractWe have previously shown that lipoma preferred partner (LPP) mediates TGFβ-induced breast cancer cell migration and invasion. Herein, we demonstrate that diminished LPP expression reduces circulating tumour cell numbers, impairs cancer cell extravasation and diminishes lung metastasis. LPP localizes to invadopodia, along with Tks5/actin, at sites of matrix degradation and at the tips of extravasating breast cancer cells as revealed by intravital imaging of the chick chorioallantoic membrane (CAM). Invadopodia formation, breast cancer cell extravasation and metastasis require an intact LPP LIM domain and the ability of LPP to interact with α-actinin. Finally, we show that Src-mediated LPP phosphorylation at specific tyrosine residues (Y245/301/302) is critical for invadopodia formation, breast cancer cell invasion and metastasis. Together, these data define a previously unknown function for LPP in the formation of invadopodia and reveal a requirement for LPP in mediating the metastatic ability of breast cancer cells.
Volumetric chemical imaging by stimulated Raman projection microscopy and tomography
Authors: Chen Xueli , Zhang Chi , Lin Peng , Huang Kai-Chih , Liang Jimin , Tian Jie , Cheng Ji-Xin 
Published: April 2017

AbstractVolumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions and at high speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living systems. We demonstrate Bessel-beam-based stimulated Raman projection (SRP) microscopy and tomography for label-free volumetric chemical imaging. Our SRP microscope enables fast quantitation of chemicals in a 3D volume through a two-dimensional lateral scan. Furthermore, combining SRP and sample rotation, we demonstrate the SRP tomography that can reconstruct the 3D distribution of chemical compositions with optical spatial resolution at a higher speed than the Gaussian-beam-based stimulated Raman scattering sectioning imaging can. We explore the potential of our SRP technology by mapping polymer particles in 3D volumes and lipid droplets in adipose cells.
Time-reversal symmetry breaking hidden order in Sr2(Ir,Rh)O4
Authors: Jeong Jaehong , Sidis Yvan , Louat Alex , Brouet Véronique , Bourges Philippe 
Published: April 2017

AbstractLayered 5d transition iridium oxides, Sr2(Ir,Rh)O4, are described as unconventional Mott insulators with strong spin-orbit coupling. The undoped compound, Sr2IrO4, is a nearly ideal two-dimensional pseudospin-1/2 Heisenberg antiferromagnet, similarly to the insulating parent compound of high-temperature superconducting copper oxides. Using polarized neutron diffraction, we here report a hidden magnetic order in pure and doped Sr2(Ir,Rh)O4, distinct from the usual antiferromagnetic pseudospin ordering. We find that time-reversal symmetry is broken while the lattice translation invariance is preserved in the hidden order phase. The onset temperature matches that of the odd-parity hidden order recently highlighted using optical second-harmonic generation experiments. The novel magnetic order and broken symmetries can be explained by the loop-current model, previously predicted for the copper oxide superconductors.
Platinum-nickel alloy excavated nano-multipods with hexagonal close-packed structure and superior activity towards hydrogen evolution reaction
Authors: Cao Zhenming , Chen Qiaoli , Zhang Jiawei , Li Huiqi , Jiang Yaqi , Shen Shouyu , Fu Gang , Lu Bang-an , Xie Zhaoxiong , Zheng Lansun 
Published: April 2017

AbstractCrystal phase regulations may endow materials with enhanced or new functionalities. However, syntheses of noble metal-based allomorphic nanomaterials are extremely difficult, and only a few successful examples have been found. Herein, we report the discovery of hexagonal close-packed Pt–Ni alloy, despite the fact that Pt–Ni alloys are typically crystallized in face-centred cubic structures. The hexagonal close-packed Pt–Ni alloy nano-multipods are synthesized via a facile one-pot solvothermal route, where the branches of nano-multipods take the shape of excavated hexagonal prisms assembled by six nanosheets of 2.5 nm thickness. The hexagonal close-packed Pt–Ni excavated nano-multipods exhibit superior catalytic property towards the hydrogen evolution reaction in alkaline electrolyte. The overpotential is only 65 mV versus reversible hydrogen electrode at a current density of 10 mA cm−2, and the mass current density reaches 3.03 mA μgPt−1 at −70 mV versus reversible hydrogen electrode, which outperforms currently reported catalysts to the best of our knowledge.
CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin
Authors: Li P , Wang Y , Mao X , Jiang Y , Liu J , Li J , Wang J , Wang R , She J , Zhang J , Yang J , Liu Y , Liu P 
Published: April 2017

AbstractThe cancer stem cell (CSC) theory depicts a special population within the cancer mass that self-renew and sustain the cancer, even if the other cells were eliminated by therapies. How CSCs acquire these unique traits is still unclear. Crumbs homolog 3 (CRB3), a member of the CRB polarity complex, has been reported to act as a tumor suppressor. Here, we detected significantly lower or negative CRB3 expression in human breast cancer tissues. Knockdown of CRB3 generated non-tumorigenic, immortalized breast epithelial cell line MCF 10A with CSC properties. Simultaneously, we found that CRB3 downregulation induced the epithelial–mesenchymal transition and activated TAZ (transcriptional co-activator with PDZ-binding motif) and β-catenin. Significantly, the activation of TAZ and β-catenin sufficed in conferring MCF 10A cells with CSC properties. This study demonstrates that cell polarity proteins may serve as a switch of the differentiated vs multipotent states in breast cancers.
Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system
Authors: Sugano Shigeo S. , Suzuki Hiroko , Shimokita Eisuke , Chiba Hirofumi , Noji Sumihare , Osakabe Yuriko , Osakabe Keishi 
Published: April 2017

AbstractMushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryopreserved protoplasts, we identified a novel promoter, CcDED1
pro
, with seven times stronger activity in this assay than the conventional promoter GPD2. To develop highly efficient genome editing using CRISPR/Cas9 in C. cinerea, we used the CcDED1
pro
to express Cas9 and a U6-snRNA promoter from C. cinerea to express gRNA. Finally, CRISPR/Cas9-mediated GFP mutagenesis was performed in a stable GFP expression line. Individual genome-edited lines were isolated, and loss of GFP function was detected in hyphae and fruiting body primordia. This novel method of high-throughput CRISPR/Cas9-based genome editing using cryopreserved protoplasts should be a powerful tool in the study of edible mushrooms.
Cation and voltage dependence of lidocaine inhibition of the hyperpolarization-activated cyclic nucleotide-gated HCN1 channel
Authors: Putrenko Igor , Yip Raymond , Schwarz Stephan K. W. , Accili Eric A. 
Published: April 2017

AbstractLidocaine is known to inhibit the hyperpolarization-activated mixed cation current (Ih) in cardiac myocytes and neurons, as well in cells transfected with cloned Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) channels. However, the molecular mechanism of Ih inhibition by this drug has been limitedly explored. Here, we show that inhibition of Ih by lidocaine, recorded from Chinese hamster ovary (CHO) cells expressing the HCN1 channel, reached a steady state within one minute and was reversible. Lidocaine inhibition of Ih was greater at less negative voltages and smaller current amplitudes whereas the voltage-dependence of Ih activation was unchanged. Lidocaine inhibition of Ih measured at −130 mV (a voltage at which Ih is fully activated) was reduced, and Ih amplitude was increased, when the concentration of extracellular potassium was raised to 60 mM from 5.4 mM. By contrast, neither Ih inhibition by the drug nor Ih amplitude at +30 mV (following a test voltage-pulse to −130 mV) were affected by this rise in extracellular potassium. Together, these data indicate that lidocaine inhibition of Ih involves a mechanism which is antagonized by hyperpolarizing voltages and current flow.
Repeated inoculation of cattle rumen with bison rumen contents alters the rumen microbiome and improves nitrogen digestibility in cattle
Authors: Ribeiro Gabriel O. , Oss Daniela B. , He Zhixiong , Gruninger Robert J. , Elekwachi Chijioke , Forster Robert J. , Yang WenZhu , Beauchemin Karen A. , McAllister Tim A. 
Published: April 2017

AbstractFuture growth in demand for meat and milk, and the socioeconomic and environmental challenges that farmers face, represent a “grand challenge for humanity”. Improving the digestibility of crop residues such as straw could enhance the sustainability of ruminant production systems. Here, we investigated if transfer of rumen contents from bison to cattle could alter the rumen microbiome and enhance total tract digestibility of a barley straw-based diet. Beef heifers were adapted to the diet for 28 days prior to the experiment. After 46 days, ~70 percent of rumen contents were removed from each heifer and replaced with mixed rumen contents collected immediately after slaughter from 32 bison. This procedure was repeated 14 days later. Intake, chewing activity, total tract digestibility, ruminal passage rate, ruminal fermentation, and the bacterial and protozoal communities were examined before the first and after the second transfer. Overall, inoculation with bison rumen contents successfully altered the cattle rumen microbiome and metabolism, and increased protein digestibility and nitrogen retention, but did not alter fiber digestibility.
Host-probiotic interaction: new insight into the role of the endocannabinoid system by in vivo and ex vivo approaches
Authors: Gioacchini Giorgia , Rossi Giacomo , Carnevali Oliana 
Published: April 2017

AbstractThe endocannabinoid system plays an important role in regulating inflammation in several chronic or anomalous gut inflammatory diseases. In vivo and ex vivo studies showed that 30 days treatment with a probiotic mix activated the endocannabinoid system in zebrafish. These results highlight the potential of this probiotic mixture to regulate immune cell function, by inducing gene expression of toll-like receptors and other immune related molecules. Furthermore, TUNEL assay showed a decrease in the number of apoptotic cells, and this finding was supported by a reduction in pro-apoptotic factors and an increase in anti-apoptotic molecules. The results presented here strengthen the molecular mechanisms activated by probiotic mix controlling immune response and inflammation.
The paradox of a long grounding during West Antarctic Ice Sheet retreat in Ross Sea
Authors: Bart Philip J. , Krogmeier Benjamin J. , Bart Manon P. , Tulaczyk Slawek 
Published: April 2017

AbstractMarine geological data show that the West Antarctic Ice Sheet (WAIS) advanced to the eastern Ross Sea shelf edge during the Last Glacial Maximum (LGM) and eventually retreated ~1000 km to the current grounding-line position on the inner shelf. During the early deglacial, the WAIS deposited a voluminous stack of overlapping grounding zone wedges (GZWs) on the outer shelf of the Whales Deep Basin. The large sediment volume of the GZW cluster suggests that the grounding-line position of the paleo-Bindschadler Ice Stream was relatively stationary for a significant time interval. We used an upper bound estimate of paleo-sediment flux to investigate the lower bound duration over which the ice stream would have deposited sediment to account for the GZW volume. Our calculations show that the cluster represents more than three millennia of ice-stream sedimentation. This long duration grounding was probably facilitated by rapid GZW growth. The subsequent punctuated large-distance (~200 km) grounding-line retreat may have been a highly non-linear ice sheet response to relatively continuous external forcing such as gradual climate warming or sea-level rise. These findings indicate that reliable predictions of future WAIS retreat may require incorporation of realistic calculations of sediment erosion, transport and deposition.
Three-stage anaerobic co-digestion of food waste and horse manure
Authors: Zhang Jingxin , Loh Kai-Chee , Lee Jonathan , Wang Chi-Hwa , Dai Yanjun , Wah Tong Yen 
Published: April 2017

AbstractA novel compact three-stage anaerobic digester (HM3) was developed to combine the advantages of high solids anaerobic digestion (AD) and wet AD for co-digestion of food waste and horse manure. By having three separate chambers in the three-stage anaerobic digester, three different functional zones were created for high-solids hydrolysis, acidogenesis and wet methanogenesis. The results showed that the functionalized partitioning in HM3 significantly accelerated the solubilization of solid organic matters and the formation of volatile fatty acids, resulting in an increase of 11~23% in methane yield. VS reduction in the HM3 presents the highest rate of 71% compared to the controls. Pyrosequencing analysis indicated that different microbial communities in terms of hydrolyzing bacteria, acidogenic bacteria and methanogenic archaea were selectively enriched in the three separate chambers of the HM3. Moreover, the abundance of the methanogenic archaea was increased by 0.8~1.28 times compared to controls.
Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
Authors: Wang Yijia , Zhang Chunze , Zhang Shiwu , Zhao Zhenying , Wang Jiawen , Song Jiali , Wang Yue , Liu Jun , Hou Shaobin 
Published: April 2017

AbstractTaxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity. Kanglaite injection is an extract from Coix lacryma-jobi seed and is usually combined with other agents to treat cancer. The aim of this study was to investigate the treatment effect of Taxol combined with Kanglaite on colorectal cancer cell lines. Kanglaite pretreatment followed by Taxol treatment was found to show the best synergism among all combination strategies. This combination also resulted in the smallest tumour volume in a Balb/c mice model. Kanglaite inhibited the expression of nuclear factor (NF)-κΒ and upregulated that of connexin 43, both of which sensitized cancer cells to Taxol. Moreover, Kanglaite increased many cellular variations caused by Taxol, including tubulin polymerization, caspase-3 cleavage, and upregulated expression of survivin and cyclin B1. These results suggest that Kanglaite pretreatment may increase the effect of Taxol on colorectal cancer.
Novel 1.5 GPa-strength with 50%-ductility by transformation-induced plasticity of non-recrystallized austenite in duplex steels
Authors: Sohn Seok Su , Song Hyejin , Jo Min Chul , Song Taejin , Kim Hyoung Seop , Lee Sunghak 
Published: April 2017

AbstractNeeds for steel designs of ultra-high strength and excellent ductility have been an important issue in worldwide automotive industries to achieve energy conservation, improvement of safety, and crashworthiness qualities. Because of various drawbacks in existing 1.5-GPa-grade steels, new development of formable cold-rolled ultra-high-strength steels is essentially needed. Here we show a plausible method to achieve ultra-high strengths of 1.0~1.5 GPa together with excellent ductility above 50% by actively utilizing non-recrystallization region and TRansformation-Induced Plasticity (TRIP) mechanism in a cold-rolled and annealed Fe-Mn-Al-C-based steel. We adopt a duplex microstructure composed of austenite and ultra-fine ferrite in order to overcome low-yield-strength characteristics of austenite. Persistent elongation up to 50% as well as ultra-high yield strength over 1.4 GPa are attributed to well-balanced mechanical stability of non-crystallized austenite with critical strain for TRIP. Our results demonstrate how the non-recrystallized austenite can be a metamorphosis in 1.5-GPa-grade steel sheet design.