bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1
Authors: Hulin Julie-Ann , Tommasi Sara , Elliot David , Hu Dong Gui , Lewis Benjamin C. , Mangoni Arduino A. 
Published: October 2017

AbstractDimethylarginine dimethylaminohydrolase 1 (DDAH1) is responsible for metabolism of an endogenous inhibitor of nitric oxide synthase (NOS), asymmetric dimethylarginine (ADMA), which plays a key role in modulating angiogenesis. In addition to angiogenesis, tumours can establish a vascular network by forming vessel-like structures from tumour cells; a process termed vasculogenic mimicry (VM). Here, we identified over-expression of DDAH1 in aggressive MDA-MB-231, MDA-MB-453 and BT549 breast cancer cell lines when compared to normal mammary epithelial cells. DDAH1 expression was inversely correlated with the microRNA miR-193b. In DDAH1+ MDA-MB-231 cells, ectopic expression of miR-193b reduced DDAH1 expression and the conversion of ADMA to citrulline. In DDAH1− MCF7 cells, inhibition of miR-193b elevated DDAH1 expression. Luciferase reporter assays demonstrated DDAH1 as a direct target of miR-193b. MDA-MB-231 cells organised into tube structures in an in vitro assay of VM, which was significantly inhibited by DDAH1 knockdown or miR-193b expression. Mechanistically, we found miR-193b regulates cell proliferation and migration of MDA-MB-231 cells, whilst DDAH1 knockdown inhibited cell migration. These studies represent the first evidence for DDAH1 expression, regulation and function in breast cancer cells, and highlights that targeting DDAH1 expression and/or enzymatic activity may be a valid option in the treatment of aggressive breast cancers.
Bi-directional regulation of acupuncture on extrahepatic biliary system: An approach in guinea pigs
Authors: Zhao Jingjun , Yu Yutian , Luo Man , Li Liang , Rong Peijing 
Published: October 2017

AbstractClinically, acupuncture affects the motility of the extrahepatic biliary tract, but the underlining mechanisms are still unknown. We applied manual acupuncture (MA) and electrical acupuncture (EA) separately at acupoints Tianshu (ST25), Qimen (LR14), Yanglingquan (GB34), and Yidan (CO11) in forty guinea pigs (4 groups) with or without atropinization under anesthesia while Sphincter of Oddi (SO) myoelectric activities and gallbladder pressure were monitored. In both MA and EA groups, stimulation at ST25 or LR14 significantly increased the frequency and amplitude of SO myoelectrical activities and simultaneously decreased the gallbladder pressure as compared to the pre-MA and pre-EA (P < 0.05). On the contrary, stimulation at GB34 or CO11 significantly decreased SO myoelectricity and increased the gallbladder pressure (P < 0.05). Pretreatment with atropine could abolish the effect of stimulation at acupoints ST25, GB34 and LR14 (P > 0.05), although significant myoelectricity increases were still inducible with MA or EA stimulation at CO11 (P < 0.05). In summary, acupuncture has bi-directional effects to gallbladder pressure and SO function, which probably due to autonomic reflex and somatovisceral interactions.
Effect of adipose-derived mesenchymal stem cell transplantation on vascular calcification in rats with adenine-induced kidney disease
Authors: Yokote Shinya , Katsuoka Yuichi , Yamada Akifumi , Ohkido Ichiro , Yokoo Takashi 
Published: October 2017

AbstractPrevious studies have investigated the use of mesenchymal stem cells (MSCs) to treat damaged kidneys. However, the effect of adipose-derived MSCs (ASCs) on vascular calcification in chronic kidney disease (CKD) is still poorly understood. In the present study, we explored the potential of ASCs for the treatment of CKD and vascular calcification. CKD was induced in male Sprague-Dawley rats by feeding them a diet containing 0.75% adenine for 4 weeks. ASCs transplantation significantly reduced serum inorganic phosphorus (Pi) as compared to that in the control. The histopathology of the kidneys showed a greater dilation of tubular lumens and interstitial fibrosis in the control group. Calcium and Pi contents of the aorta in the ASCs transplantation group were lower than those in the control group. Von Kossa staining of the thoracic aorta media revealed that ASCs transplantation suppressed vascular calcification. Thus, this study revealed that autogenic ASCs transplantation inhibits kidney damage and suppresses the progression of vascular calcification in the CKD rat model, suggesting that autogenic ASCs transplantation is a novel approach for preventing the progression of CKD and vascular calcification.
Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens
Authors: Chu Jun , Zhang Qiang , Zuo Zonghui , El-Ashram Saeed , Guo Yongxia , Zhao Peng , Huang Shujian , He Cheng , Khan Ahrar 
Published: October 2017

AbstractSince 2007, most areas of China have seen outbreaks of poultry airsacculitis, which causes hugely economic losses to the poultry industry. However, there are no effective measures to combat the problem. In this study, 105 rations were collected to isolate Aspergillus spp. from the diseased farms. In subsequent experiments, SPF chickens were inoculated with Ornithobacterium rhinotracheale (ORT), Chlamydia psittaci (C. psittaci) and Aspergillus fumigatus (A. fumigatus), and mortality rate, body weight gain and lesion score were evaluated. Of these ration samples, 63 (60.0%) were A. fumigates, 21 (20.0%) were Aspergillus niger (A. niger) and 11 (10.5%) were Aspergillus candidus (A. candidus). Furthermore, SPF birds infected with C. psittaci, ORT, H9N2 virus and A. fumigatus conidia exhibited a mortality rate of 40%, while simultaneous co-infection with C. psittaci, ORT and A. fumigatus resulted in a mortality rate of 20%. The avian airsacculitis was manifested in the C. psittaci + ORT/A. fumigatus, C. psittaci + H9N2 + ORT/A. fumigatus and C. psittaci + H9N2/A. fumigatus groups while others had transient respiratory diseases without mortality. Our survey indicates that feed-borne A. fumigatus is prevalent in poultry rations. The combination of C. psittaci, ORT, H9N2 and A. fumigatus conidia contributes to the replication of avian airsacculitis by aggravating the severe damage to the air sacs and lungs of chickens.
New reverse genetics and transfection methods to rescue arboviruses in mosquito cells
Authors: Atieh Thérèse , Nougairède Antoine , Klitting Raphaëlle , Aubry Fabien , Failloux Anna-Bella , de Lamballerie Xavier , Priet Stéphane 
Published: October 2017

AbstractReverse genetics is a critical tool to decrypt the biological properties of arboviruses. However, whilst reverse genetics methods have been usually applied to vertebrate cells, their use in insect cells remains uncommon due to the conjunction of laborious molecular biology techniques and of specific difficulties surrounding the transfection of such cells. To leverage reverse genetics studies in both vertebrate and mosquito cells, we designed an improved DNA transfection protocol for insect cells and then demonstrated that the simple and flexible ISA (Infectious Subgenomic Amplicons) reverse-genetics method can be efficiently applied to both mammalian and mosquito cells to generate in days recombinant infectious positive-stranded RNA viruses belonging to genera Flavivirus (Japanese encephalitis, Yellow fever, West Nile and Zika viruses) and Alphavirus (Chikungunya virus). This method represents an effective option to potentially overcome technological issues related to the study of arboviruses.
Language learning in the adult brain: disrupting the dorsolateral prefrontal cortex facilitates word-form learning
Authors: Smalle Eleonore H. M. , Panouilleres Muriel , Szmalec Arnaud , Möttönen Riikka 
Published: October 2017

AbstractAdults do not learn languages as easily as children do. It has been hypothesized that the late-developing prefrontal cortex that supports executive functions competes with procedural learning mechanisms that are important for language learning. To address this hypothesis, we tested whether a temporary neural disruption of the left Dorsolateral Prefrontal Cortex (DLPFC) can improve implicit, procedural learning of word-forms in adults. Young adults were presented with repeating audio-visual sequences of syllables for immediate serial recall in a Hebb repetition learning task that simulates word-form learning. Inhibitory theta-burst Transcranial Magnetic Stimulation was applied to the left DLPFC or to the control site before the Hebb task. The DLPFC-disrupted group showed enhanced learning of the novel phonological sequences relative to the control group. Moreover, learning was negatively correlated with executive functions that rely on the DLPFC in the control group, but not in the DLPFC-disrupted group. The results support the hypothesis that a mature prefrontal cortex competes with implicit learning of word-forms. The findings provide new insight into the competition between brain mechanisms that contribute to language learning in the adult brain.
The morphology of antennal lobe projection neurons is controlled by a POU-domain transcription factor Bmacj6 in the silkmoth Bombyx mori
Authors: Namiki Shigehiro , Fujii Tsuguru , Shimada Toru , Kanzaki Ryohei 
Published: October 2017

AbstractHow to wire a neural circuit is crucial for the functioning of the nervous system. Here, we describe the neuroanatomy of the olfactory neurons in the spli mutant strain of silkmoth (Bombyx mori) to investigate the function of a transcription factor involved in neuronal wiring in the central olfactory circuit. The genomic structure of the gene Bmacj6, which encodes a class IV POU domain transcription factor, is disrupted in the spli mutant. We report the neuroanatomical abnormality in the morphology of the antennal lobe projection neurons (PNs) that process the sex pheromone. In addition to the mis-targeting of dendrites and axons, we found axonal bifurcation within the PNs. These results indicate that the morphology of neurons in the pheromone processing pathway is modified by Bmacj6.
Molecular phylogeny and divergence times of Astragalus section Hymenostegis: An analysis of a rapidly diversifying species group in Fabaceae
Authors: Bagheri Ali , Maassoumi Ali Asghar , Rahiminejad Mohammad Reza , Brassac Jonathan , Blattner Frank R. 
Published: October 2017

AbstractThe taxa of Astragalus section Hymenostegis are an important element of mountainous and steppe habitats in Southwest Asia. A phylogenetic hypothesis of sect. Hymenostegis has been obtained from nuclear ribosomal DNA internal transcribed spacer (ITS) and plastid ycf1 sequences of up to 303 individuals from 106 species, including all 89 taxa currently assigned to sect. Hymenostegis, 14 species of other Astragalus sections, and two species of Oxytropis and one Biserrula designated as outgroups. Bayesian phylogenetic inference and parsimony analyses reveal that three species from two other closely related sections group within sect. Hymenostegis, making the section paraphyletic. DNA sequence diversity is generally very low among Hymenostegis taxa, which is consistent with recent diversification of the section. We estimate that diversification in sect. Hymenostegis occurred in the middle to late Pleistocene, with many species arising only during the last one million years, when environmental conditions in the mountain regions of Southwest and Central Asia cycled repeatedly between dry and more humid conditions.
International Outcome Inventory for Hearing Aids: Data From a Large Swedish Quality Register Database
Authors: Arlinger Stig , Nordqvist Peter , Öberg Marie 
Published: October 2017

Purpose
The purpose of this study was to analyze a database of completed International Outcome Inventory for Hearing Aids (IOI-HA) questionnaires obtained from over 100,000 clients fitted with new hearing aids in Sweden during the period of 2012–2016. Mean IOI-HA total scores were correlated with degree of hearing loss, unilateral versus bilateral fitting, first-time versus return clients, gender, and variation among dispensing clinics. The correlations with expectations, service quality, and technical functioning of the hearing aids were also analyzed.

Method
Questionnaires containing the 7 IOI-HA items as well as questions concerning some additional issues were mailed to clients 3–6 months after fitting of new hearing aids. The questionnaires were returned to and analyzed by an independent research institute.

Results
More than 100 dispensing clinics nationwide take part in this project. A response rate of 52.6% resulted in 106,631 data sets after excluding incomplete questionnaires. Forty-six percent of the responders were women, and 54% were men. The largest difference in mean score (0.66) was found for the IOI-HA item “use” between return clients and first-time users. Women reported significantly higher (better) scores for the item “impact on others” compared with men. The bilaterally fitted subgroup reported significantly higher scores for all 7 items compared with the unilaterally fitted subgroup. Experienced users produced higher scores on benefit and satisfaction items, whereas first-time users gave higher scores for residual problems. No correlation was found between mean IOI-HA total score and average hearing threshold level (pure-tone average [PTA]). Mean IOI-HA total scores were found to correlate significantly with perceived service quality of the dispensing center and with the technical functionality of the hearing aids.

Conclusions
When comparing mean IOI-HA total scores from different studies or between groups, differences with regard to hearing aid experience, gender, and unilateral versus bilateral fitting have to be considered. No correlation was found between mean IOI-HA total score and degree of hearing loss in terms of PTA. Thus, PTA is not a reliable predictor of benefit and satisfaction of hearing aid provision as represented by the IOI-HA items. Identification of a specific lower fence in PTA for hearing aid candidacy is therefore to be avoided. Large differences were found in mean IOI-HA total scores related to different dispensing centers.
Diagnosing and Screening in a Minority Language: A Validation Study
Authors: Zokoll Melanie A. , Wagener Kirsten C. , Kollmeier Birger 
Published: October 2017

Purpose
The Turkish Digit Triplet Test for hearing self-screening purposes and the Turkish Matrix Test (TURMatrix) for follow-up hearing diagnostics offer an automated closed-set response format where patients respond by choosing from response alternatives. Their applicability for testing Turkish-speaking patients in their native language by German audiologists with different Turkish language skills was investigated.

Method
Tests were composed of spoken numbers (Turkish Digit Triplet Test) or sentences (TURMatrix). For 49 participants differing in hearing ability, speech reception thresholds (SRTs) in noise and quiet were obtained, for the TURMatrix with either the open- or closed-set response format, by audiologists with and without Turkish language skills, respectively.

Results
SRTs of both tests correlate closely with each other as well as with hearing ability, but not as closely as individual SRTs in quiet with hearing ability. SRTs in noise of listeners with normal hearing were about 0.7 dB lower for the closed-set than for the open-set response format.

Conclusions
The 2 tests yield comparable results and are applicable to professionals without suitable language skills. For the closed-set response format of the TURMatrix, literacy is crucial and supplemental (visual) cues improve performance. Speech audiometry in noise should assess suprathreshold processing deficits independently from language proficiency in the majority language.
Auditory Scene Analysis: An Attention Perspective
Authors: Sussman Elyse S. 
Published: October 2017

Purpose
This review article provides a new perspective on the role of attention in auditory scene analysis.

Method
A framework for understanding how attention interacts with stimulus-driven processes to facilitate task goals is presented. Previously reported data obtained through behavioral and electrophysiological measures in adults with normal hearing are summarized to demonstrate attention effects on auditory perception—from passive processes that organize unattended input to attention effects that act at different levels of the system. Data will show that attention can sharpen stream organization toward behavioral goals, identify auditory events obscured by noise, and limit passive processing capacity.

Conclusions
A model of attention is provided that illustrates how the auditory system performs multilevel analyses that involve interactions between stimulus-driven input and top-down processes. Overall, these studies show that (a) stream segregation occurs automatically and sets the basis for auditory event formation; (b) attention interacts with automatic processing to facilitate task goals; and (c) information about unattended sounds is not lost when selecting one organization over another. Our results support a neural model that allows multiple sound organizations to be held in memory and accessed simultaneously through a balance of automatic and task-specific processes, allowing flexibility for navigating noisy environments with competing sound sources.

Presentation Video

http://cred.pubs.asha.org/article.aspx?articleid=2601618
Cortical and Sensory Causes of Individual Differences in Selective Attention Ability Among Listeners With Normal Hearing Thresholds
Authors: Shinn-Cunningham Barbara 
Published: October 2017

Purpose
This review provides clinicians with an overview of recent findings relevant to understanding why listeners with normal hearing thresholds (NHTs) sometimes suffer from communication difficulties in noisy settings.

Method
The results from neuroscience and psychoacoustics are reviewed.

Results
In noisy settings, listeners focus their attention by engaging cortical brain networks to suppress unimportant sounds; they then can analyze and understand an important sound, such as speech, amidst competing sounds. Differences in the efficacy of top-down control of attention can affect communication abilities. In addition, subclinical deficits in sensory fidelity can disrupt the ability to perceptually segregate sound sources, interfering with selective attention, even in listeners with NHTs. Studies of variability in control of attention and in sensory coding fidelity may help to isolate and identify some of the causes of communication disorders in individuals presenting at the clinic with “normal hearing.”

Conclusions
How well an individual with NHTs can understand speech amidst competing sounds depends not only on the sound being audible but also on the integrity of cortical control networks and the fidelity of the representation of suprathreshold sound. Understanding the root cause of difficulties experienced by listeners with NHTs ultimately can lead to new, targeted interventions that address specific deficits affecting communication in noise.

Presentation Video

http://cred.pubs.asha.org/article.aspx?articleid=2601617
Perceptions of Cluttering Among Communication Sciences and Disorders and Non Communication Sciences and Disorders Students
Authors: Blanchet Paul G. , Snyder Greg 
Published: October 2017

Purpose
The aim of this study was to compare perceptions of cluttering of undergraduate Communication Sciences and Disorders majors with perceptions of non-majors.

Method
A total of 79 undergraduate students served as participants; 37 students were Communications Sciences and Disorders majors enrolled in an introductory-level course. The other 42 students were recruited from nutrition courses in the Department of Family and Consumer Sciences. All students were provided with a written definition of cluttering and also viewed a short segment of an educational video. Students then rated a person who clutters on a variety of speech skills and personality scales.

Results
Results revealed that the Communication Sciences and Disorders students rated a person who clutters as exhibiting a significantly more inappropriate speech rate than did the Family and Consumer Sciences majors. However, results revealed no significant group differences in ratings of any personality traits.

Conclusions
Findings do not support the presence of relatively positive perceptions of cluttering among Communication Sciences and Disorders majors, at least not prior to coursework or clinical training. Further research is needed to replicate these findings with larger sample sizes, and to assess attitudes of these students before and after coursework and/or clinical training in fluency disorders.
Star formation history of Canis Major OB1
Authors: Santos-Silva T. , Gregorio-Hetem J. , Montmerle T. , Fernandes B. , Stelzer B. 
Published: October 2017

Aims. The Canis Major OB1 Association has an intriguing scenario of star formation, especially in the region called Canis Major R1 (CMa R1) traditionally assigned to a reflection nebula, but in reality an ionized region. This work is focussed on the young stellar population associated with CMa R1, for which our previous results from ROSAT, optical, and near-infrared data had revealed two stellar groups with different ages, suggesting a possible mixing of populations originated from distinct star formation episodes.
Methods. The X-ray data allow the detected sources to be characterized according to hardness ratios, light curves, and spectra. Estimates of mass and age were obtained from the 2MASS catalogue and used to define a complete subsample of stellar counterparts for statistical purposes.
Results. A catalogue of 387 XMM-Newton sources is provided, of which 78% are confirmed as members or probable members of the CMa R1 association. Flares (or similar events) were observed for 13 sources and the spectra of 21 bright sources could be fitted by a thermal plasma model. Mean values of fits parameters were used to estimate X-ray luminosities. We found a minimum value of log(LX [erg/s] ) = 29.43, indicating that our sample of low-mass stars (M⋆ ≤ 0.5 M⊙), which are faint X-ray emitters, is incomplete. Among the 250 objects selected as our complete subsample (defining our “best sample”), 171 are found to the east of the cloud, near Z CMa and dense molecular gas, of which 50% of them are young (<5 Myr) and 30% are older (>10 Myr). The opposite happens to the west, near GU CMa, in areas lacking molecular gas: among 79 objects, 30% are young and 50% are older. These findings confirm that a first episode of distributed star formation occurred in the whole studied region ~10 Myr ago and dispersed the molecular gas, while a second, localized episode (<5 Myr) took place in the regions where molecular gas is still present.