bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of thePlasmodium falciparumhomologue of Vps16, a member of the Vps-C tethering complex
Authors: Galaup Thomas , Lauruol Florian , Sergerie Audrey , Richard Dave 
Published: October 2024

ABSTRACTThe organelles of the apical complex (rhoptries, micronemes and dense granules) are critical for erythrocyte invasion by the malaria parasitePlasmodium falciparum. Though they have essential roles in the parasite lifecycle, the mechanisms behind their biogenesis are still poorly defined. The Class C Vps proteins Vps11, 16, 18 and 33 constitute the core of the CORVET and HOPS complexes implicated in vesicle tethering and fusion in the eukaryotic endolysosomal system. Work in the model apicomplexanToxoplasma gondiihas revealed that TgVps11 is essential for the generation of the apical complex.P. falciparumpossesses all the four subunits of the Vps-C complex but their function is currently unknown. We here present an initial characterization of theP. falciparumorthologue of Vps16, a member of the Vps-C complex. Our structural predictions suggest that the structure of PfVps16 is similar to its other eukaryotic counterparts and that the binding region responsible for its interaction with PfVps33 is conserved. We next show that PfVps16 is expressed throughout the asexual erythrocytic cycle and that it is potentially associated with the rhoptries in schizont stage parasites. Finally, we present our unsuccessful attempts at studying its function using knock sideways.
DNA topoisomerase I acts as supercoiling sensor for transcription elongation inE. coli
Authors: Vidmar Vita , Borde Céline , Bruno Lisa , Takacs Maria , Batisse Claire , Saint-André Charlotte , Zhu Chengjin , Espéli Olivier , Lamour Valérie , Weixlbaumer Albert 
Published: October 2024

AbstractWhen DNA is transcribed to RNA, the DNA double helix is constantly unwound and rewound to provide access for RNA polymerase (RNAP). This induces DNA supercoiling as a function of transcript length due to over- and under-twisting of the DNA downstream and upstream of RNAP, respectively. Using single-particle cryo-EM andin vivoassays we investigated the relationship between bacterial RNAP and DNA Topoisomerase I (TopoI), which removes negative supercoils accumulating upstream of RNAP. TopoI binds to relaxed DNA upstream of RNAP in a manner suggesting a sensory role awaiting the formation of negative supercoils and involving a conformational switch in the functional domains of TopoI. On DNA substrates mimicking negatively supercoiled DNA, TopoI threads one strand into the active site for cleavage while binding the complementary strand with an auxiliary domain. We propose a comprehensive model for DNA relaxation in the context of a transcribing RNAP.
Impact of soil moisture on microbial diversity and their enzyme activity on agricultural soil
Authors: Bogati Kalisa , Sewerniak Piotr , Walczak Maciej 
Published: October 2024

AbstractIn this study, the impact of a two-month of drought stress on the microbial abundance, their enzymes and functional diversity in four agricultural soil (Gniewkowo (G), Lulkowo (L), Nieszawa (N), and Suchatówka (S) sites from Poland) was investigated during summer season. The physicochemical parameters (pH, organic carbon (C), calcium carbonate (CaCO3), total nitrogen (N), nitrate (NO3-), ammonium (NH4+), total phosphorus (P), available phosphate (P2O5)), and specific biological parameters (microbial abundance, CLPP, and soil enzymes (phosphatases (acid; ACP and alkaline; AKP), dehydrogenase (DH), and urease (UR)) were conducted on the soil samples in this study. The physicochemical and biological data were compared between zero-week (T0) and 8thweek (T8) time intervals. The microbial enumeration showed higher bacterial populations (496.63 x 104CFU g-1dry soil) compared to actinomycetes (13.43 x 104CFU g−1dry soil), and lowest were the fungal population (67.68 x 102CFU g-1dry soil) at T8. The functional diversity showed strong positive significance in the G, N and S sites at T8. On the contrary, most of the L sites showed negative significance with the utilization of amines only, by the end of the experiment. Overall, the microbial population, their enzymes and functional diversity showed positive correlation with soil moisture content in all four investigated sites. The findings of our study indicate that soil biological activities in agricultural regions can be modified by a mere two months of drought.
Structural extension of the human exocyst is enabled by a minimal interface
Authors: Xu Haonan D. , Badonyi Mihaly , Sopipong Watanyoo , MacGowan Stuart A. , Marsh Joseph A. , Murray David H. 
Published: October 2024

AbstractIn multicellular organisms, the machinery responsible for polarized trafficking directs constitutive cargo secretion at distinct sites of the plasma membrane, cilia, and junctional structures. Central to this machinery is the exocyst complex, which tethers cargo vesicles to their destination membrane, alongside other intracellular membrane tethering roles. Precisely how the exocyst spatially integrates membranes and membrane resident binding partners is unclear. Here, we address the structural morphology and formation of the human exocyst complex. Through structural approaches coupled to predictive models, we determined that the exocyst and its subcomplexes have extended ‘arm-like’ structures that help maximize its reach. Moreover, we demonstrate minimal intersubunit interaction, in contrast to prior models. Nucleation of the holocomplex occurs through a single site, explaining its spatial extension. Our results provide the biochemical basis for exocyst complex assembly, suggesting an ornate extended architecture.SignificanceCargo transport to the eukaryotic cell plasma membrane predominantly relies on the exocyst as a central, signal-integrating polarized trafficking complex. How this single cargo vesicle tethering complex can target diverse vesicles to destination membrane is poorly understood, partly due to inconsistent structural models. Here, we show an extended architecture of the human exocyst complex, revealing a minimal nucleation interface between its two subcomplexes. The distinct morphology of human exocyst, compared to yeast models, suggests a novel mechanism that supports its versatile role in membrane trafficking.
Tapering-enhanced high-efficiency THz waveguide oscillator
Authors: Yang Y. N. , Fisher A. , Musumeci P. , Li R. K. , Kravchenko M. , Agustsson R. , Chen Y. , Murokh A. 
Published: October 2024

Using a waveguide in a THz FEL has been shown to maximize the coupling and enable efficient extraction of relativistic electron beam energy from a single passage through a tapered helical undulator. An oscillator configuration can further boost energy extraction above the single-pass limits and open the door toward very high average power THz sources. Embedding the undulator in an oscillator cavity is particularly useful in combination with high repetition rate electron sources, even if at reduced peak brightness, since recycling a fraction of the radiation as an intense seed can compensate for lower single-pass gain. In this paper, we investigate the efficiency scaling of a tapering-enhanced waveguide oscillator, showcasing its capability for frequency-tuning operation and high-efficiency operation at different wavelengths. Using a thermionic-driven beamline equipped with compression elements, numerical start-to-end simulation results indicate a 16% efficiency at 200 GHz and a 2.1% efficiency at 1.5 THz, resulting in kW-level average power out-coupled in radiation pulses with few hundred μJ energy and tens of MW peak power.

Published by the American Physical Society
2024
Search for the process
e+e−→η′γ
in the energy range
s=1.075−2  GeV
Authors: Achasov M. N. , Barnyakov A. Yu. , Bedarev E. V. , Beloborodov K. I. , Berdyugin A. V. , Bogdanchikov A. G. , Botov A. A. , Dimova T. V. , Druzhinin V. P. , Zhabin V. N. , Zharinov Yu. M. , Kardapoltsev L. V. , Kasaev A. S. , Kattsin A. A. , Kyrpotin A. N. , Kovrizhin D. P. , Koop I. A. , Korol A. A. , Kupich A. S. , Kryukov A. P. , Melnikova N. A. , Muchnoy N. Yu. , Obrazovsky A. E. , Pakhtusova E. V. , Pugachev K. V. , Rastigeev S. A. , Rogovsky Yu. A. , Senchenko A. I. , Serednyakov S. I. , Silagadze Z. K. , Surin I. K. , Usov Yu. V. , Kharlamov A. G. , Chistyakov D. E. , Shatunov Yu. M. , Sherstyuk S. P. , Shtol D. A. ,   SND Collaboration
Published: October 2024

The results of the search for the e+e−→η′γ process in the center-of-mass energy range from 1.075 to 2 GeV are presented. We analyze data with an integral luminosity of 746  pb−1 accumulated with the SND detector at the e+e− collider VEPP-2000 in 2010–2024. The η′→ηπ0π0 decay is used in the analysis, with the subsequent η and π0 decays into γγ. Upper limits not exceeding 13 pb have been set on the e+e−→η′γ cross section at the 90% confidence level.

Published by the American Physical Society
2024
Fermi surface and Lifshitz transitions of a ferromagnetic superconductor under external magnetic fields
Authors: Leenen R. , Aoki D. , Knebel G. , Pourret A. , McCollam A. 
Published: October 2024

Lifshitz transitions are being increasingly recognized as significant in a wide variety of strongly correlated and topological materials, and understanding the origin and influence of Lifshitz transitions is leading to deeper understanding of key aspects of magnetic, transport, or quantum critical behavior. In the ferromagnetic superconductor UCoGe, a magnetic field applied along the c axis has been shown to induce a series of anomalies in both transport and thermopower that may be caused by Lifshitz transitions. The need to understand the subtleties of the relationship between magnetism, superconductivity and a heavy-electron Fermi surface in the ferromagnetic superconductors makes it important to explore if and why a series of magnetic-field-induced Lifshitz transitions occurs in UCoGe. Here we report magnetic susceptibility measurements of UCoGe, performed at temperatures down to 45 mK and magnetic fields (μ0H||c) up to 30 T. We observe a series of clearly defined features in the susceptibility, and multiple sets of strongly field-dependent de Haas-van Alphen oscillations, from which we extract detailed field dependence of the quasiparticle properties. We complement our experimental results with density functional theory band structure calculations, and include a simple model of the influence of magnetic field on the calculated Fermi surface. By comparing experimental and calculated results, we determine the likely shape of the Fermi surface and identify candidate Lifshitz transitions that could correspond to two of the features in susceptibility. We connect these results to the development of magnetization in the system.

Published by the American Physical Society
2024
DBA as an enabler of future management practice – case study insights and reflections from recent graduates
Authors: Kearney Arthur , Harrington Denis , Dempsey David , Collings David 
Published: October 2024

Purpose
The purpose of the study is to explore a Doctorate in Business Administration (DBA) programme in the context of a small emerging Irish university as an enabler for future management practice development.

Design/methodology/approach
The research comprises a literature review and a single case study focused on a single DBA graduate of a DBA programme embedded in a new technological Irish university.

Findings
Findings indicate that the DBA supports a unique form of managerial learning supportive of the development of future management practice in graduates. Specifically, such managerial learning emerges from the embracement of differences by both faculty and students; guidance by faculty in opening students to uncertainty and unknowing and in supporting students towards becoming architects of socio-technical organisational change. Ultimately, the DBA graduate is not viewed as leaving the programme, with novel management practices that are time-bound, rather they are imbued with a learning potential supportive of continual management practice development into the future. Theoretically, the authors extend studies that indicate the potential of the DBA as a mechanism for management practice development, indicating the role of managerial learning in context.

Research limitations/implications
The research is limited to a single case study of a DBA programme embedded in a new technological Irish university and draws from the perspective of one key informant graduate. Hence, future research into how managerial learning impacts new management practice development in the context of other universities is encouraged.

Practical implications
The central role of managerial learning promoted in the DBA within this new technological university indicates two practical implications of the research. First, both organisations and students considering DBA education as a means of developing future management practices are encouraged to contemplate such education through the lens of managerial learning. It is anticipated that such contemplation will improve preparedness. Second, business schools are encouraged to contemplate improving their programmes through the managerial learning lens, with such improvement activating potential in marketing the DBA to both students and organisations.

Originality/value
Theoretically the authors extend existing studies that indicate the potential of the DBA as a mechanism for management practice development. Specifically the role of contextual managerial learning is articulated, and is argued to support a management practice development capable of continual transformation.
Research on wind turbines preventive maintenance strategies based on reliability and cost-effectiveness ratio
Authors: Li Jian Hua , Jia Shilin , Ren Lina , Li Xueliang 
Published: October 2024

Purpose
The purpose of this study is to optimize the operational efficiency of the entire system by developing a reasonable maintenance strategy for wind turbines that improves component reliability and safety while reducing maintenance costs.

Design/methodology/approach
A hybrid incomplete preventive maintenance (PM) model based on boundary intensity process is established to give dynamic PM intervals for wind turbines using an iterative method with reliability as a constraint; the selection method of PM and replacement is given based on the cost-effectiveness ratio, which in turn determines the optimal number of PM for wind turbines.

Findings
The reliability is used to obtain the components’ maintenance cycle, and the cost-effectiveness ratio is used to select the number of maintenance times, thus, getting the optimal maintenance strategy. The validity of this paper’s method is verified by arithmetic cases, which provides a new method for formulating a reasonable PM strategy for wind turbines.

Practical implications
The wind turbine preventive maintenance strategy for Boundary intensity process proposed in this paper can scientifically formulate the maintenance strategy, optimize the cost-effectiveness per unit of time of the wind power generation system, and solve the problems of difficulty in formulating a reasonable maintenance strategy for the wind turbine components and high operation and maintenance costs.

Originality/value
In this paper, the authors describe the failure pattern by a Boundary intensity process, establish a hybrid incomplete PM model by introducing a failure intensity increment factor and an age reduction factor and establish a maintenance strategy optimization model with comprehensive consideration of reliability and cost-effectiveness ratio. Finally, the validity of the model in this paper is verified by arithmetic case analysis.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-05-2024-0153/
EMSA-IK: a real-time evolutionary multi-objective semi-analytical inverse kinematics algorithm for redundant manipulators
Authors: Chen Zhaoyang , Min Kang , Fan Xinyang , Tu Baoxu , Ni Fenglei , Liu Hong 
Published: October 2024

Purpose
This paper aims to propose a real-time evolutionary multi-objective semi-analytical inverse kinematics (IK) algorithm (EMSA-IK) for solving the multi-objective IK of redundant manipulators.

Design/methodology/approach
Within EMSA-IK, the parameterization method is applied to reduce the number of optimization variables of the evolutionary algorithm and calculate semi-analytical solutions that meet high target pose accuracy. The original evolutionary algorithm is improved with the proposed adaptive search sub-space strategy so that the improved evolutionary algorithm can be used to efficiently perform global search within the parametric joint space to obtain the global optimal parametric joint angles that satisfy multi-objective constraints.

Findings
Ablation experiments show the effectiveness of the improved strategy used for evolutionary algorithms. Comparative experiments on different manipulators demonstrate the advantages of EMSA-IK in terms of generalizability and balancing multiple objectives, for example, motion continuity, joint limits and obstacle avoidance. Real-world experiments further validate the effectiveness of the proposed algorithm for real-time application.

Originality/value
The semi-analytical IK solution that simultaneously satisfies high target pose accuracy and multi-objective constraints can be obtained in real time. Compared to existing semi-analytical IK algorithms, the proposed algorithm achieves obstacle avoidance for the first time. The proposed algorithm demonstrates superior generalizability, applicable to not only redundant manipulators with revolute joints but also those with prismatic joints.
Pilot study of an anti-racist professional development: effects on educators’ multicultural attitudes and efficacy
Authors: Stoffers Melissa , Barnes Tia Navelene , Strickland Lauren , Jung Joanne , Branch Kira , Perry Danika , Hatchimonji Danielle 
Published: October 2024

Purpose
This study aims to understand the impact of a pilot of the actions against racism (AAR) intervention, aimed at enhancing educators’ multicultural efficacy and attitudes in a mid-Atlantic K-8 charter school.

Design/methodology/approach
AAR included eight sessions delivered over one school year. Experienced facilitators led these sessions, focusing on combating racism, prejudice and discrimination. Of the 84 school staff, 56 completed a baseline survey and 33 completed a postintervention survey.

Findings
Paired sample t-tests revealed a significant increase in multicultural attitudes, t(29) = 2.55, p = 0.016, whereas the increase in multicultural efficacy was not significant, t(28) = 1.93, p = 0.063. The authors examined cultural and emotional intelligence as moderators of the intervention’s impact. Higher baseline cognitive reappraisal scores (an indicator of emotional intelligence) were linked to a larger increase in multicultural efficacy from pre- to postintervention, B = −0.59, t (27) = −2.20, p = 0.037. The number of sessions attended was unrelated to the intervention’s impact.

Research limitations/implications
This study was a single-site, uncontrolled pilot of AAR with a small sample size. Further research in additional settings with appropriately powered samples is needed to validate these results and extend findings to examine the impact of AAR on the student experience.

Originality/value
The study demonstrates the promising potential of AAR in improving teachers' multicultural attitudes and efficacy. Exploratory findings highlight the role of cognitive reappraisal in enhancing multicultural efficacy, contributing valuable insights for designing effective teacher training programs. This research supports the implementation of critical, identity-centered and asset-based pedagogies in education.
Empowering resilience through digital transformation intentions: synergizing knowledge sharing and transformational leadership amid COVID-19
Authors: Yang Zhibo , Dong Ming , Guo Hailan , Peng Weibin 
Published: October 2024

PurposeThis study examines the role of digital transformation intentions in enhancing the perceived resilience of firms, with a focus on China’s manufacturing sector. It investigates the mediating role of knowledge sharing and the moderating impact of transformational leadership.Design/methodology/approachA quantitative approach was employed, collecting data from 347 manufacturing firms. Participants included managers and MBA students involved in digital transformation projects. The study utilized statistical analysis to explore the relationships between digital transformation intentions, knowledge sharing, transformational leadership and perceived firm resilience.FindingsThe analysis reveals that knowledge sharing is a critical mediating factor between digital transformation intentions and perceived firm resilience. Additionally, transformational leadership significantly strengthens this relationship, highlighting its importance in the successful implementation of digital initiatives.Research limitations/implicationsThe study is geographically and sectorally limited to China’s manufacturing sector, which may affect the generalizability of the findings. Future research could explore other sectors and regions to validate and extend the results.Practical implicationsThe findings underscore the necessity of integrating digital transformation initiatives with effective leadership and knowledge management practices. Firms that foster transformational leadership and facilitate knowledge sharing are better equipped to enhance their resilience in the face of global disruptions.Originality/valueThis research offers a deep understanding of how digital transformation intentions, mediated by knowledge sharing and supported by transformational leadership, contribute to perceived firm resilience. It provides valuable insights for both academic research and practical applications in the field of management.
Reuse of service concept elements for modular service design
Authors: Hunke Fabian , Satzger Gerhard , Tuunanen Tuure 
Published: October 2024

PurposeThis research investigates the systematic reuse of service concept elements within modular service design, aiming to offer actionable insights into effective conceptualization of services and extending methodological underpinnings to enhance the approach of service design.Design/methodology/approachEmploying a design science research approach, this study investigates the intentional and targeted reuse of service concept elements for modular service design. It develops four general design principles and applies them in a real-world context to demonstrate and evaluate the purposeful integration of service concept elements.FindingsThis research reveals the efficacy of reusing service concept elements for modular service design, highlighting the benefits of this approach in conceptualizing new services. It theorizes generalizable design knowledge by formalizing four design principles that allow to underpin the reuse of service concept elements.Originality/valueThis research contributes to service design literature by providing actionable insights into the systematic reuse of service concept elements, particularly within the framework of modular service design. We develop and test general design principles and, specifically, apply them for analytics-based digital services.