bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions
Authors: Senthilnathan N. , Chandaluri Ch. G. , Radhakrishnan T. P. 
Published: August 2017

AbstractThe enhanced fluorescence emission of diaminodicyanoquinodimethanes (DADQs) in rigid and aggregated states holds great promise for bioimaging applications. This is demonstrated through their efficient application in epidermal and stomatal imaging with selective staining of cell walls and nuclei. Major advantages include the small quantities (a few nmols) of the fluorophore required, choice of DADQs soluble in water and organic solvents, and quick staining of the specimen in buffer-free state and in buffer medium. The molecular level interactions that enable staining are unraveled through isothermal calorimetry, infra-red spectroscopy and microscopy with energy dispersive X-ray spectroscopy analysis. It is proposed that DADQs with ionic or H-bonding functionalities bind to the polygalacturonic acid moieties in the epidermal layer; the former can bind also to nucleic acid polyanions. Fluorescence experiments explain the emission enhancement that enables the efficient imaging. DADQs are easy to synthesize, non-cytotoxic, and thermally, chemically and photo-stable, requiring no special storage conditions; preliminary experiments point to their potential utility in imaging different classes of cells.
Plasmonic trapping of nanoparticles by metaholograms
Authors: Rui Guanghao , Ma Yanbao , Gu Bing , Zhan Qiwen , Cui Yiping 
Published: August 2017

AbstractManipulation of nanoparticles in solution is of great importance for a wide range of applications in biomedical, environmental, and material sciences. In this work, we present a novel plasmonic tweezers based on metahologram. We show that various kinds of nanoparticles can be stably trapped in a surface plasmon (SP) standing wave generated by the constructive interference between two coherent focusing SPs. The absence of the axial scattering force and the enhanced gradient force enable to avoid overheating effect while maintaining mechanical stability even under the resonant condition of the metallic nanoparticle. The work illustrates the potential of such plasmonic tweezers for further development in lab-on-a-chip devices.
Perspektive Heilpraktiker
Authors: Ocker Sandra 
Published: August 2017

ZusammenfassungZunehmend legen Therapeuten die Prüfung zur Heilpraktikererlaubnis ab.
Dahinter stecken die eingeschränkten Therapiemöglichkeiten und die momentan
diffizile gesetzlichen Lage der Osteopathie in Deutschland, vermutet Dozentin
Sandra Ocker von der Heilpraktikerschule Homo Et Sanitas, Giengen/Brenz. Sie
zeigt die Chancen auf und beschreibt den Weg zum Ziel.
Spezielle urologische Traumatologie bei Kindern und Jugendlichen
Authors: Faé P. , Schröder A. , Rösch W. , Stein R. 
Published: August 2017

Zusammenfassung Isolierte Verletzungen des Urogenitaltraktes sind besonders bei Kindern und Jugendlichen selten. Das am häufigsten betroffene Organ ist die Niere, vorwiegend im Rahmen von Politraumen. Insgesamt ist der Urogenitaltrakt bei etwa 3 % aller von Politraumen betroffenen Kinder beteiligt. Die Literatur sowie die internationalen Leitlinien geben klare therapeutische Konzepte über die Diagnose und Behandlung von Nierentraumen vor. Die häufigsten isolierten Urogenitalverletzungen bei Kindern und Jugendlichen betreffen allerdings das äußere Genital und werden durch stumpfe Traumen, Schürfungen, Quetschungen sowie durch Pfählungen verursacht. Für diese Formen von Verletzungen gibt es aufgrund der Vielfalt der Verletzungsmuster keine allgemein gültigen Empfehlungen oder Leitlinien. In unseren Fallberichten kinder- und jugendurologischer Patienten gehen wir auf die speziellen Formen von Genitaltraumen und auf deren individuelle Diagnostik und Therapie ein.
Kollagenosen – Krankheiten mit vielen Gesichtern
Authors: Voll Reinhard 
Published: August 2017

Liebe Leserinnen und Leser der Aktuellen Rheumatologie,
die Kollagenosen stellen eine recht uneinheitliche Gruppe systemischer Autoimmunopathien des Bindegewebes dar, die aufgrund ihrer oft unspezifischen (Früh-)Symptome und vielgestaltigen Manifestationen nicht selten verkannt werden. Finden die Patientinnen, betroffen sind ganz überwiegend Frauen, schließlich den Weg zum Rheumatologen, so kann auch dieser nicht immer gleich eine definitive Diagnose stellen. Undifferenzierte Formen sind in der Frühphase häufig, auch rechtzeitige therapeutische Interventionen dürften gelegentlich verhindern, dass die Erkrankung fortschreitet und somit einer bestimmten Entität zugeordnet werden kann.
Lepra
Authors: Effendy I. 
Published: August 2017

ZusammenfassungLepra ist eine chronische bakterielle Infektionskrankheit durch Mycobacaterium leprae, derzeit leiden weltweit ca. 3 Millionen Personen. Lepra ist nicht hoch kontagiös, für die Übertragung steht die Tröpfcheninfektion im Vordergrund. Die vielfältigen klinischen Symptome sind durch den Immunstatus des Betroffenen, die aktuelle Bakterienladung sowie Bestandsdauer der Krankheit vor der Diagnosestellung bestimmt. Zwecks Behandlungsstrategie wird die Krankheit in bakterienarme (tuberkuloid, borderline-tuberkuloid) und bakterienreiche (borderline-borderline, borderline-lepromatöse und lepromatöse) Formen unterteilt. Erregernachweis erfolgt durch mikroskopisches Direktpräparat, Histopathologie bzw. mittels PCR. Die Kombinationstherapie mit Dapson, Rifampicin und Clofazimin gilt als Standard (WHO).
Weizensensitivität: FODMAP-armer Urweizen als Alternative
Authors: Kautz Sybille 
Published: August 2017

ZusammenfassungWer nach dem Verzehr von getreidehaltigen Produkten gastrointestinale Beschwerden entwickelt,
kann außer an Zöliakie oder Weizenallergie auch an einer Weizensensitivität oder einem
Reizdarmsyndrom leiden. Auslöser dafür ist bei manchen Betroffenen eine Unverträglichkeit
gegen fermentierbare Oligo-, Di- und Monosaccharide und Polyole (FODMAP) aus bestimmten
Getreidearten wie Weizen, einigen Obst- und Gemüsesorten sowie aus Hülsenfrüchten. Diese
Personen können von einer FODMAP-armen Ernährung profitieren. Der Urweizen 2ab enthält
weniger FODMAP als herkömmlicher Weizen und ausschließlich das meist besser verträgliche
Gluten des AABB-Genoms. Damit hergestellte Backwaren können in eine FODMAP-arme Diät
integriert werden, sodass sensitive Personen nicht auf Weizenprodukte verzichten
müssen.
Exploring regulation in tissues with eQTL networks
Authors: Fagny Maud , Paulson Joseph N. , Kuijjer Marieke L. , Sonawane Abhijeet R. , Chen Cho-Yi , Lopes-Ramos Camila M. , Glass Kimberly , Quackenbush John , Platig John 
Published: August 2017

Significance
A core tenet in genetics is that genotype influences phenotype. In an individual, the same genome can be expressed in substantially different ways, depending on the tissue. Expression quantitative trait locus (eQTL) analysis, which associates genetic variants at millions of locations across the genome with the expression levels of each gene, can provide insight into genetic regulation of phenotype. In each of 13 tissues we performed an eQTL analysis, represented significant associations as edges in a network, and explored the structure of those networks. We found clusters of eQTL linked to shared functions across tissues and tissue-specific clusters linked to tissue-specific functions, driven by genetic variants with tissue-specific regulatory potential. Our findings provide unique insight into the genotype–phenotype relationship.
Experimental and statistical reevaluation provides no evidence forDrosophilacourtship song rhythms
Authors: Stern David L. , Clemens Jan , Coen Philip , Calhoun Adam J. , Hogenesch John B. , Arthur Ben J. , Murthy Mala 
Published: August 2017

SignificancePrevious studies have reported that male vinegar flies sing courtship songs with a periodic rhythm of approximately 55 s. Several years ago, we showed that we could not replicate this observation. Recently, the original authors have claimed that we failed to find rhythms because (i) our flies did not sing enough and (ii) our software for detecting song did not detect all song events. They reported that they could detect rhythms in song annotated by hand. We show here that we cannot replicate their observation of rhythms in the hand-annotated data or in other datasets. We also show that our original methods were not biased against detecting rhythms. We conclude that song rhythms cannot be detected.
TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER–Golgi intermediate compartments
Authors: Hanna Michael G. , Block Samuel , Frankel E. B. , Hou Feng , Johnson Adam , Yuan Lin , Knight Gavin , Moresco James J. , Yates John R. , Ashton Randolph , Schekman Randy , Tong Yufeng , Audhya Anjon 
Published: August 2017

Significance
The endoplasmic reticulum (ER) serves as a platform for the packaging of most secretory proteins into conserved coat protein complex II (COPII)-coated transport carriers destined for ER–Golgi intermediate compartments (ERGIC) in animal cells. In this work, we demonstrate that Trk-fused gene (TFG), a protein implicated in multiple neurodegenerative diseases and oncogenesis, functions in this pathway by interacting directly with the COPII protein Sec23. Specifically, we show that TFG outcompetes interactions between the inner and outer layers of the COPII coat, indicating that TFG promotes the uncoating process after transport carriers undergo scission from the ER. Moreover, we demonstrate that TFG simultaneously captures and concentrates COPII transport carriers at the ER/ERGIC interface to enable the rapid movement of secretory cargoes to the ERGIC.
A rescaled expansiveness for flows
Authors: Wen Xiao , Wen Lan 
Published: August 2017

We introduce a new version of expansiveness for flows. Let

M
M

be a compact Riemannian manifold without boundary and let

X
X

be a

C
1

C^1

vector field on

M
M

that generates a flow

φ
t

\varphi _t

on

M
M

. We call

X
X

rescaling expansive on a compact invariant set

Λ
\Lambda

of

X
X

if for any

ϵ
>
0

\epsilon >0

there is

δ
>
0

\delta >0

such that, for any

x
,
y
∈
Λ

x,y\in \Lambda

and any time reparametrization

θ
:

R

→

R

\theta :\mathbb {R}\to \mathbb {R}

, if

d
(

φ
t

(
x
)
,

φ

θ
(
t
)

(
y
)
)
≤
δ
‖
X
(

φ
t

(
x
)
)
‖

d(\varphi _t(x), \varphi _{\theta (t)}(y))\le \delta \|X(\varphi _t(x))\|

for all

t
∈

R

t\in \mathbb R

, then

φ

θ
(
t
)

(
y
)
∈

φ

[
−
ϵ
,
ϵ
]

(

φ
t

(
x
)
)

\varphi _{\theta (t)}(y)\in \varphi _{[-\epsilon , \epsilon ]}(\varphi _t(x))

for all

t
∈

R

t\in \mathbb R

. We prove that every multisingular hyperbolic set (singular hyperbolic set in particular) is rescaling expansive and that a converse holds generically.
In Utero ART Exposure and Birth and Early Growth Outcomes Among HIV-Exposed Uninfected Infants Attending Immunization Services: Results From National PMTCT Surveillance, South Africa
Authors: Ramokolo Vundli , Goga Ameena E , Lombard Carl , Doherty Tanya , Jackson Debra J , Engebretsen Ingunn MS 
Published: August 2017

Abstract

Background
Despite the recognized benefit of antiretroviral therapy (ART) for preventing and treating HIV, some studies have reported adverse birth outcomes with in utero ART exposure. We evaluated the effect of infant in utero HIV and ART exposure on preterm delivery (PTD), low birth weight (LBW), small for gestational age (SGA), and underweight for age (UFA) at 6 weeks.

Methods
We surveyed 6179 HIV-unexposed-uninfected (HUU) and 2599 HIV-exposed-uninfected (HEU) infants. HEU infants were stratified into 3 groups: ART, Zidovudine alone, and no antiretrovirals (None). The ART group was further stratified to explore pre- or postconception exposure. Multivariable logistic regression evaluated effects of HIV and ARV exposure on the outcomes.

Results
We found higher odds of PTD, LBW, SGA, and UFA in HEU than HUU infants. HEU in the None group (adjusted odds ratio [AOR], 1.9; 95% confidence interval [CI], 1.2–3.0) or those whose mothers initiated ART preconception (AOR, 1.7; 95% CI, 1.1–2.5) had almost twice the odds of PTD than infants whose mothers started ART postconception, but no increased odds for other outcomes.

Conclusions
There was an association between preconception ART and PTD. As ART access increases, pregnancy registers or similar surveillance should be in place to monitor outcomes to inform future policy.
Family-centered Care Improved Neonatal Medical and Neurobehavioral Outcomes in Preterm Infants: Randomized Controlled Trial
Authors: Yu Yen-Ting , Hsieh Wu-Shiun , Hsu Chyong-Hsin , Lin Yuh-Jyh , Lin Chyi-Her , Hsieh Shulan , Lu Lu , Cherng Rong-Ju , Chang Ying-Ju , Fan Pi-Chuan , Yao Nai-Jia , Chen Wei J. , Jeng Suh-Fang 
Published: August 2017

Abstract

Background
Family-centered care for preterm infants in Western societies has yielded short- to medium-term benefits. However, the intervention effects have rarely been validated in Eastern societies.

Objective
The aim of this study was to examine whether a family-centered intervention program (FCIP) could improve the short-term medical and neurobehavioral outcomes in preterm infants with very low birth weight (VLBW; a birth weight of &lt;1,500 g) in Taiwan over the outcomes seen with a usual care program (UCP).

Design
This was a multicenter, single-blind, randomized controlled trial study.

Setting
Three medical centers in northern and southern Taiwan were the locations for the study.

Participants
The participants were 251 VLBW preterm infants without severe perinatal complications.

Intervention
The infants were randomly assigned to receive the FCIP or the UCP during hospitalization.

Measurements
Infant morbidities, feeding, growth, and neurobehavioral performance were evaluated during the neonatal period. Parental adherence to interventions was measured in the FCIP group.

Results
The FCIP promoted earlier full enteral feeding (β = –1.1 weeks; 95% CI = –1.9 to –0.2 weeks) and hospital discharge (β = –0.6 week; 95% CI = –1.1 to –0.1 weeks), greater weight gain (β = 3.3 g/d; 95% CI = 0.1 to 6.6 g/d), and better neurobehavioral performance than the UCP (β = 1.2 points; 95% CI = 0.2 to 2.3 points). Furthermore, a higher degree of parental motivation in interventions, goal attainment, and comprehensiveness of home activities was significantly associated with greater effects in infants’ neurobehavioral performance and weight gain (r = .20–.31; all Ps &lt; .05).

Limitations
The findings may not be generalized to preterm infants with severe perinatal diseases and parents with a low level of interest in interventions.

Conclusions
Family-centered care facilitated short-term medical and neurobehavioral outcomes in VLBW preterm infants in Taiwan; the effects were likely achieved through parental adherence to interventions. The designated strategies may be considered in a future launch of family-centered care in Taiwan.
PAs in primary care
Authors: Coplan Bettie , Smith Noel , Cawley James F. 
Published: August 2017

ABSTRACT

Objectives:
Understanding the PA primary care workforce is an initial step toward greater use of primary care PAs in new healthcare delivery models. This study sought to describe primary care PA practice as it compares with PA practice in other specialties.

Methods:
Data from two 2015 national American Academy of Physician Assistants surveys were analyzed using descriptive statistics. Statistically significant differences between primary care and specialty PAs were assessed using tests of column proportions and tests of column means.

Results:
Compared with PAs in specialties, primary care PAs were older, saw more patients per week, and spent less time consulting with physicians. In addition, higher percentages were Hispanic, had a record of military service, and had plans to leave their specialty or retire.

Conclusions:
Primary care PAs appear to possess unique strengths; however, challenges to maintaining a primary care PA workforce are substantial.
Is there a role for inhaled anticholinergic therapy in asthma management?
Authors: Parkey Shannon M. , Mospan Cortney M. 
Published: August 2017

ABSTRACT
Anticholinergic therapy has long been a cornerstone of management of chronic obstructive pulmonary disease (COPD) but has not been included in treatment guidelines for asthma. In September 2015, tiotropium bromide was approved for use in adults with asthma; the indication has since been expanded to children ages 6 years and older. This article discusses appropriate patient selection and dosing, and the role of tiotropium bromide in asthma management.
Dual-Task Walking Performance in Older Persons With Hearing Impairment: Implications for Interventions From a Preliminary Observational Study
Authors: Wollesen Bettina , Scrivener Katharine , Soles Kirsty , Billy Yaw , Leung Angela , Martin Felicity , Iconomou Nicholas , McMahon Catherine , Dean Catherine 
Published: August 2017

Objectives:
Adults with “hearing loss” have an increased falls risks. There may be an association between hearing impairment and walking performance under dual-task (DT) and triple-task (TT) conditions. The aim of this study was to identify DT and TT effects on walking speed, step length, and cadence in adults with hearing impairment, previous falls, and physical limitations.

Design:
The observational study included 73 community-dwelling older people seeking audiology services. Data were collected on sociodemographic characteristics, previous falls, fear of falling, physical limitations, and walking performance under three task conditions. Differences between the task conditions (single task [ST], DT, and TT) and the hearing groups were analyzed with a two-way ANOVA with repeated measures. The influence of fall risks and limited physical functioning on walking under ST, DT, and TT conditions was analyzed with ANOVAs, with ST, DT, and TT performance as repeated measurement factor (i.e., walking speed, step length and Cadence × Previous falls, or short physical performance battery <12 × Hearing Groups).

Results:
Walking speed was reduced accompanied by decreased step length and increased cadence in people with more severe hearing loss. Larger negative effects on DT and TT walking were found with increasing hearing loss (speed and cadence decreased with higher DT costs). Highest DT costs were found for the walking-manual conditions. These results were accompanied by small effects of older age and more comorbidities.

Conclusions:
This first screening data of walking performance under different conditions for people with hearing loss warrants the need for development and investigation of training interventions to improve walking abilities. DT training may be beneficial to enhance motor and cognitive flexibility and to reduce fall risks.
Correlation Between Original Biopsy Pathology and Mohs Intraoperative Pathology
Authors: Stiegel Evan , Lam Charlene , Schowalter Michael , Somani Ally-Khan , Lucas Jennifer , Poblete-Lopez Christine 
Published: August 2017

BACKGROUND
The pathology observed on initial biopsy specimens for nonmelanoma skin cancer (NMSC), specifically the tumor subtype, weighs heavily into the selection of the treatment modality. Recognizing aggressive features on intraoperative Mohs micrographic surgery (MMS) frozen sections that were not indicated on initial biopsy reports is not uncommon. To date, there is limited data describing this discrepancy.

OBJECTIVE
To investigate the frequency and nature of incongruity between the pathology seen in initial biopsies and intraoperative Mohs sections, providing information that will potentially impact skin cancer treatment and biopsy recommendations.

METHODS
Retrospective chart review of cases of NMSC referred for MMS at the authors' institution over a 1-year period, recording changes between tumor subtypes observed at biopsy and those observed during MMS.

RESULTS
Cases (50.5%) (n = 163) changed aggressiveness. Of these, 33% (n = 108) were more aggressive, whereas 17% (n = 55) were less aggressive.

CONCLUSION
A substantial discrepancy was observed between preoperative biopsy and intraoperative pathology, including a significant portion tumors that became more aggressive. These results suggest that treatment modalities without margin control may not provide adequate treatment for a considerable number of NMSC based on preoperative tumor type alone.
Nucleoligands-repurposing G Protein–coupled Receptor Ligands to Modulate Nuclear-localized G Protein–coupled Receptors in the Cardiovascular System
Authors: Audet Nicolas , Dabouz Rabah , Allen Bruce G. , Hébert Terence E. 
Published: August 2017

Abstract:There is significant evidence that internal pools of G protein–coupled receptors (GPCRs) exist and may be affected by both endogenous signaling molecules and hydrophobic pharmaceutical ligands, once assumed to only affect cell surface versions of these receptors. Here, we discuss evidence that the biology of nuclear GPCRs in particular is complex, rich, and highly interactive with GPCR signaling from the cell surface. Caging existing GPCR ligands may be an excellent means of further stratifying the phenotypic effects of known pharmacophores such as β-adrenergic, angiotensin II, and type B endothelin receptor ligands in the cardiovascular system. We describe some synthetic strategies we have used to design ligands to go from in cellulo to in vivo experiments. We also consider how surface and intracellular GPCR signaling might be integrated and ways to dissect this. If they could be selectively targeted, nuclear GPCRs and their associated nucleoligands would represent a completely novel area for exploration by Pharma.
Drug-induced Myopia and Bilateral Angle Closure Secondary to Zolmitriptan
Authors: Lee Jonathan Tak Loong , Skalicky Simon Edward , Lin Ming-Lee 
Published: August 2017

Purpose:
To describe a unique case of drug-induced transient myopia with angle-closure glaucoma in a patient being treated with zolmitriptan for migraines.

Methods:
A 42-year-old woman who had been using increasing amounts of zolmitriptan over the previous 12 months presented with an acute myopic shift and raised intraocular pressures (IOP) with anterior chamber shallowing. Clinical examination findings at presentation and at follow-up visits were reviewed.

Results:
Initial examination revealed unaided visual acuities of 20/100 in the right eye and 20/125 in the left, with IOP measuring 34 mm Hg bilaterally. Zolmitriptan was ceased and the patient was commenced on topical antiglaucoma medication. Within 2 weeks, IOP had normalized, with deepening of the anterior chambers and complete resolution of her myopia. Her final recorded unaided visual acuities were 20/12.5 in the right eye and 20/16 in the left. When topical antiglaucoma medication was ceased the patient developed pressure-related headaches and selective laser trabeculoplasty was performed to minimize the need for long-term topical medication use.

Conclusion:
Idiosyncratic drug reactions resulting in ciliochoroidal effusion, secondary angle closure, and transient myopia are well described, but they have not been previously reported with zolmitriptan use. An awareness of the various potential causative agents is important, as findings are generally reversible if recognized early and if the offending drug is discontinued.