bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dryopteris crassirhizomaDryocrassinABBAfor Postharvest Control of the Potato Dry Rot PathogenFusarium solanivar. coeruleum
Authors: Wang Wenzhong , Gao Rui , Bo Zhigang , Chen Lingling , Min Fanxiang , Gao Yunfei , Yang Shuai , Wei Qi , Dong Xuezhi , Lv Dianqiu , Chang Ying 
Published: April 2017

AbstractDryopteris crassirhizomadryocrassinABBAtreatment was tested for its effectiveness in controllingFusarium solanivar. coeruleumgrowthin vitroand for prevention of postharvest dry rot of potato tubers and slices. DryocrassinABBAstrongly inhibited mycelial growth, resulting in reductions in both mycelium dry weight and per cent spore germination ofF. solanivar.coeruleumat concentrations of 2.0, 0.5 and 0.1 mg/ml. Scanning electron microscopy (SEM) observations showed that treatment induced abnormal, tightly twisted morphological changes in hyphae. Moreover,in vivoexperiments demonstrated that dryocrassinABBAtreatment at 2 mg/mleffectively controlled dry rot of potato tubers inoculated with mycelial discs ofF. solanivar.coeruleum. After dryocrassinABBAtreatment, the content of soluble proteins, peroxidase (POD) and superoxide dismutase (SOD) activity increased, and malondialdehyde (MDA) content remained the stable situation. In addition, the expression level of plant lipid‐transfer proteins (LTPs) genes –StLTPa1,StLTPa7,StLTPb1andStLTPb3 –was upregulated. These results collectively demonstrate that dryocrassinABBAcan inhibit growth ofFusariumpathogens to induce disease resistance. On the other side, dryocrassinABBAmaybe induce potato defence responses.
Effects of nanocalcium carbonate on egg production performance and plasma calcium of laying hens
Authors: Ganjigohari S. , Ziaei N. , Ramzani Ghara A. , Tasharrofi S. 
Published: April 2017

SummaryThis experiment was conducted to evaluate the effects of nanocalcium carbonate (NCC) instead of calcium carbonate (CC) on egg production, egg weight, egg mass, FCR, blood calcium and egg quality characteristics in laying hens. A total of 120 laying hens were used in a 10‐weeks trial, from week 23 to 33 of age. Laying hens were randomly assigned to six treatments with four replications, five hens each. The experimental treatments involved replacing 50% of the CC in the diet by decreasing amounts of NCC and were T1 Basal diet (BD) with 8.06% CC; T2 (6.045% of CC as a negative control); T3 (4.03% of CC replaced by 2.015% NCC); T4 (4.03% of CC replaced by 1.01% NCC); T5 (4.03% of CC replaced by 0.252% NCC) and T6 (4.03 of CC replaced with 0.126%NCC).Egg weight was unaffected by dietary treatments (p > .05). However, the egg production percentage and egg mass in T6 were less than that of other treatments (p < .05). The laying hens in the control group had the best average feed conversion ratio (p < .05). Also, the lowest concentration of calcium in hens’ blood was recorded for birds fed T6 (p < .05). The best egg shell quality (relative egg shell weight and egg shell weight/surface) was observed in T1 (p < .05).Collectively, our results demonstrated that NCC could replace CC at a lower inclusion level but extreme reduction of calcium concentration in diets (to 1.43% Ca in the T6 group) reduced production performance, egg quality characteristics, Tibia thickness and blood calcium of laying hens.
Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact‐dependent translocation
Authors: Gordon Jay E. , Costa Tiago R. D. , Patel Roosheel S. , Gonzalez‐Rivera Christian , Sarkar Mayukh K. , Orlova Elena V. , Waksman Gabriel , Christie Peter J. 
Published: April 2017

SummaryRecent studies have shown that conjugation systems of Gram‐negative bacteria are composed of distinct inner and outer membrane core complexes (IMCs and OMCCs, respectively). Here, we characterized the OMCC by focusing first on a cap domain that forms a channel across the outer membrane. Strikingly, the OMCC caps of the Escherichia coli pKM101 Tra and Agrobacterium tumefaciens VirB/VirD4 systems are completely dispensable for substrate transfer, but required for formation of conjugative pili. The pKM101 OMCC cap and extended pilus also are dispensable for activation of a Pseudomonas aeruginosa type VI secretion system (T6SS). Chimeric conjugation systems composed of the IMCpKM101 joined to OMCCs from the A. tumefaciens VirB/VirD4, E. coli R388 Trw, and Bordetella pertussis Ptl systems support conjugative DNA transfer in E. coli and trigger P. aeruginosa T6SS killing, but not pilus production. The A. tumefaciens VirB/VirD4 OMCC, solved by transmission electron microscopy, adopts a cage structure similar to the pKM101 OMCC. The findings establish that OMCCs are highly structurally and functionally conserved – but also intrinsically conformationally flexible – scaffolds for translocation channels. Furthermore, the OMCC cap and a pilus tip protein coregulate pilus extension but are not required for channel assembly or function.
The physiological phosphorylation of tau is critically changed in fetal brains of individuals with Down syndrome
Authors: Milenkovic I. , Jarc J. , Dassler E. , Aronica E. , Iyer A. , Adle‐Biassette H. , Scharrer A. , Reischer T. , Hainfellner J. A. , Kovacs G. G. 
Published: April 2017

AimsDown syndrome (DS) is a common cause of mental retardation accompanied by cognitive impairment. Comprehensive studies suggested a link between development and ageing, as nearly all individuals with DS develop Alzheimer disease (AD)‐like pathology. However, there is still a paucity of data on tau in early DS to support this notion.MethodsUsing morphometric immunohistochemistry we compared tau phosphorylation in normal brains and in brains of individuals with DS from early development until early postnatal life.ResultsWe observed in DS a critical loss of physiological phosphorylation of tau. Rhombencephalic structures showed prominent differences between controls and DS using antibodies AT8 (Ser‐202/Thr‐205) and AT180 (Thr‐231). In contrast, in the subiculum only a small portion of controls deviated from DS using antibodies AT100 (Thr‐212/Ser‐214) and AT270 (Thr‐181). With exception of the subiculum, phosphorylation‐independent tau did not differ between groups, as confirmed by immunostaining for the HT‐7 antibody (epitope between 159 and 163 of the human tau) as well.DiscussionOur observations suggest functional tau disturbance in DS brains during development, rather than axonal loss. This supports the role of tau as a further important player in the pathophysiology of cognitive impairment in DS and related AD.
Age‐associated changes in the blood‐brain barrier: comparative studies in human and mouse
Authors: Goodall E. F. , Wang C. , Simpson J. E. , Baker D. J. , Drew D. R. , Heath P. R. , Saffrey M. J. , Romero I. A. , Wharton S. B. 
Published: April 2017

AimsWhile vascular pathology is a common feature of a range of neurodegenerative diseases, we hypothesized that vascular changes occur in association with normal ageing. Therefore, we aimed to characterize age‐associated changes in the blood–brain barrier (BBB) in human and mouse cohorts.MethodsImmunohistochemistry and Evans blue assays were used to characterize BBB dysfunction (tight junction protein expression and serum plasma protein accumulation), vascular pathology (pericyte loss and vascular density) and glial pathology (astrocyte and microglial density) in ageing neurological control human prefrontal cortex (a total of 23 cases from 5 age groups representing the spectrum of young adult to old age: 20–30 years, 31–45 years, 46–60 years, 61–75 years and 75+) and C57BL/6 mice (3 months, 12 months, 18 months and 24 months, n = 5/6 per group).ResultsQuantification of the tight junction protein ZO‐1 within the cortex and cerebellum of the mouse cohort showed a significant trend to both increased number (cortex P < 0.001, cerebellum P < 0.001) and length (cortex P < 0.001, cerebellum P < 0.001) of junctional breaks associated with increasing age. GFAP expression significantly correlated with ageing in the mice (P = 0.037). In the human cohort, assessment of human protein accumulation (albumin, fibrinogen and human IgG) demonstrated cells morphologically resembling clasmatodendritic astrocytes, indicative of BBB dysfunction. Semiquantitative assessment of astrogliosis in the cortex expression revealed an association with age (P = 0.003), while no age‐associated changes in microglial pathology, microvascular density or pericyte coverage were detected.ConclusionsThis study demonstrates BBB dysfunction in normal brain ageing, both in human and mouse cohorts.
CiMYB17, a stress‐induced chicory R2R3‐MYB transcription factor, activates promoters of genes involved in fructan synthesis and degradation
Authors: Wei Hongbin , Bausewein Anja , Greiner Steffen , Dauchot Nicolas , Harms Karsten , Rausch Thomas 
Published: April 2017

Summary

In Cichorium intybus, inulin metabolism is mediated by fructan‐active enzymes (FAZYs): sucrose:sucrose 1‐fructosyltransferase (1‐SST), fructan:fructan 1‐fructosyltransferase (1‐FFT), and fructan 1‐exohydrolases 1, 2a and 2b (1‐FEH1, ‐2a and ‐2b), respectively. While these enzymes have been rigorously characterized, the transcriptional network orchestrating their development‐ and stress‐related expression has remained largely unknown.

Here, the possible role of R2R3‐MYB transcription factors in FAZY regulation was explored via bioinformatic identification of R2R3‐MYBs (using an RNA sequencing (RNAseq) database), studies of co‐expression of these factors with target genes, in vivo transient transactivation assays of FAZY target promoters (dual luciferase assay), and a yeast one‐hybrid assay investigating the specificity of the binding of these factors to cis‐elements.

The chicory MYB transcription factor CiMYB17 specifically activated promoters of 1‐SST and 1‐FFT by binding to the consensus DNA‐motif DTTHGGT. Unexpectedly, CiMYB17 also activated promoters of fructan exohydrolase genes. The stimulatory effect on promoter activities of sucrose transporter and cell wall invertase genes points to a general role in regulating the source–sink relationship.

Co‐induction of CiMYB17 with 1‐SST and 1‐FFT (and, less consistently, with 1‐FEH1/2) in nitrogen‐starved or abscisic acid (ABA)‐treated chicory seedlings and in salt‐stressed chicory hairy roots supports a role in stress‐induced fructan metabolism, including de novo fructan synthesis and trimming of pre‐existing fructans, whereas the reduced expression of CiMYB17 in developing taproots excludes a role in fructan accumulation under normal growth conditions.
An evaluation of vitamin D levels in children with seasonal allergic rhinitis during pollen season
Authors: Kutluğ Seyhan , Kılıç Mehtap , Bilgici Birşen , Paksu Şule , Yıldıran Alişan , Sancak Recep 
Published: April 2017

AbstractBackgroundSerum vitamin D levels have not been studied in children with seasonal allergic rhinitis (SAR). The aim of this study was to evaluate the vitamin D levels of children with SAR and to compare them to levels in healthy children during pollen season.MethodsThis study was conducted in 100 children with SAR and 100 healthy controls. Clinical and laboratory evaluations and vitamin D analyses of all the participants were performed between the months of April and July. Pollen sensitization was detected in the patient group using a skin prick test. 25(OH)D3 levels were compared between the patient and control groups. Associations among the patient 25(OH)D3 levels and their demographic, clinical, and laboratory characteristics were analyzed.ResultsOverall, 72% of the patients were male, the median age was 12.35 years (range: 6‐17.8 years), and the median body mass index value was 19.15 (range: 13.6‐27.8). There were no differences between the patients and healthy controls in terms of gender, age, or body mass index. The mean levels of 25(OH)D3 (20.78±6) in patients were higher than those of the controls (17.92±4). In the patient group, no associations were found among 25(OH)D3 levels, demographic characteristics, atopy test results, atopy history, severity of rhinitis, and the total four symptoms score (all P>.05).ConclusionsDuring pollen season, children with SAR may have higher vitamin D levels than healthy controls. The presence of asthma and/or atopic dermatitis in addition to SAR did not change this result.
Oxidative stress in asthmatic and non‐asthmatic adolescent swimmers—A breathomics approach
Authors: Couto Mariana , Barbosa Corália , Silva Diana , Rudnitskaya Alisa , Delgado Luís , Moreira André , Rocha Sílvia M. 
Published: April 2017

AbstractWe hypothesize that oxidative stress induced by trichloramine exposure during swimming could be related to etiopathogenesis of asthma among elite swimmers.AimTo investigate the effect of a swimming training session on oxidative stress markers of asthmatic compared to non‐asthmatic elite swimmers using exhaled breath (EB) metabolomics.MethodsElite swimmers annually screened in our department (n=27) were invited and those who agreed to participate (n=20, of which 9 with asthma) had EB collected (Tedlar® bags) before and after a swimming training session. SPME fiber (DVB/CAR/PDMS) was used to extract EB metabolites followed by a multidimensional gas chromatography analysis (GC×GC‐ToFMS). Dataset comprises eight metabolites end products of lipid peroxidation: five aliphatic alkanes (nonane, 2,2,4,6,6‐pentamethylheptane, decane, dodecane, and tetradecane) and three aldehydes (nonanal, decanal, and dodecanal). To assess exercise impact on lipid peroxidation markers, data were analyzed using principle component analysis (PCA), which was run on the original data set and on the data set constructed using differences in the metabolite total areas before and after exercise session.ResultsHeatmap representation revealed that metabolites content decreased after exercise, both for control and asthma groups; however, the greater decrease was observed for controls. Asthmatics and controls did not form separated clusters; however, control swimmers demonstrated a more varied response to the exercise being dispersed along all score plot.ConclusionIn well‐trained athletes, swimming is associated with a decrease in oxidative stress markers independently of the presence of asthma, although a more pronounced decrease was seen in controls.
Pediatric psoriasis: Should we be concerned with comorbidity? Cross‐sectional study
Authors: Kelati Awatef , Baybay Hanane , Najdi Adil , Zinoune Safae , Mernissi Fatima Z 
Published: April 2017

AbstractBackgroundSimilarly to psoriasis in adults, recent research has linked psoriasis to several comorbidities in children. The aim of this study was therefore to describe comorbidities associated with pediatric psoriasis, to investigate their relationship with psoriasis characteristics and severity, and to perform a review of the literature.MethodsA cross‐sectional study was performed on a sample of Moroccan children with psoriasis, in 2014–2016.ResultsA total of 64 pediatric psoriasis patients had metabolic comorbidities in association with psoriasis; 20 children had non‐metabolic comorbidities; and 76 children had no comorbidity. The metabolic comorbidities were as follows: abdominal obesity, 40% (n = 64); overweight, 12.5% (n = 20); metabolic syndrome, 3.7% (n = 6); and dyslipidemia, 3.1% (n = 5); the non‐metabolic comorbidities were atopy, 4.3% (n = 7); epilepsy, 3.1% (n = 5); celiac disease, 1.8% (n = 3); vitiligo, 1.8% (n = 3); alopecia ariata, 0.6% (n = 1); and valvular cardiopathy, 0.6% (n = 1). No cases of diabetes mellitus, obesity, or high blood pressure were recorded. Significant factors associated with metabolic comorbidity were extended psoriasis vulgaris >10% (P = 0.01; OR, 2.19), severe psoriasis especially pustular and erythroderma (P = 0.018; OR, 2), nail involvement (P = 0.016; OR, 1.5), face involvement (P = 0.01; OR, 1,59), resistance to topical treatment (P = 0.003; OR, 2.5) and alteration of quality of life (P = 0.02; OR, 1,7). There was no significant risk factor associated with non‐metabolic comorbidity.ConclusionsGiven the frequent association of pediatric psoriasis with many disorders, these comorbidities should be investigated and identified so that they can be taken into account in the management of psoriasis in order to avoid treatment failure. Regular follow up should be carried out in patients at risk of metabolic comorbidity.
Perfusion monitoring and intraventricular hemorrhage in preterm infants
Authors: Ishiguro Akio 
Published: April 2017

AbstractCardiovascular instability in preterm infants during the early postnatal period correlates with the development of intraventricular hemorrhage (IVH). Due to the correlation between hypotension and fluctuation of blood pressure, treatment was targeted specifically at hypotension to prevent IVH, but this was not successful. Recently, several novel perfusion markers have been found to be correlated with the development of IVH, and they are of current interest in cardiovascular management. In this review, the correlation between IVH and conventional, as well as novel, perfusion markers is examined.
Support provided by nurses to parents of hospitalized children – cultural adaptation and validation of Nurse Parent Support Tool and initial research results
Authors: Aftyka Anna , Rozalska‐Walaszek Ilona , Wróbel Aleksandra , Bednarek Anna , Dąbek Katarzyna , Zarzycka Danuta 
Published: April 2017

IntroductionChild's illness and hospitalisation are difficult and stressful situations both for the patients themselves and for their parents. Supporting the ill child and their guardians is an indispensable element of caring for ill children. The Nurse‐Parent Support Model developed by Margaret Miles is based on four elements, namely emotional support, appraisal support, informational support and instrumental support.AimThe aim of the research was (i) cultural adaptation and validation of The Nurse‐Parent Support Tool into Polish and (ii) initial assessment of parents’ perception of nursing support given to the parents of children hospitalised in five paediatric wards in Poland.Material and methodQuantitative cross‐sectional study was conducted in a group of 195 parents of children hospitalised in five different hospital wards in eastern Poland.Results and conclusionsTheoretical validity of the four‐factor version ofNPSTis proved by the correlation matrix analysis and inter‐correlation between the dimensions of the described tool as well as the analysis of the internal structure of the test verified on the basis of its internal validity which also confirms its reliability. However, theoretical validity of the test is not confirmed by the factor analysis whose results indicate that the tool encompasses two factors that explain 58.5% of variances. Respondents rated instrumental support provided by the nurses the highest, appraisal and informational support were rated slightly lower, and emotional support was rated the lowest. Negative correlation between the level of stress and emotional support (r = −0.35), informational support (r = −0.29) and support in general (r = −0.30) was demonstrated. Polish four‐factor version ofNPSTis recommended only for international comparative analyses, whereas the use of two‐factor version ofNPSTis recommended for national research programmes. The level of support given to the parents of children hospitalised in Poland seems to be unsatisfactory.
Combined aerobic and resistance training decreases inflammation markers in healthy men
Authors: Ihalainen J. K. , Schumann M. , Eklund D. , Hämäläinen M. , Moilanen E. , Paulsen G. , Häkkinen K. , Mero A. A. 
Published: April 2017

Our primary aim was to study the effects of 24 weeks of combined aerobic and resistance training performed on the same day or on different days on inflammation markers. Physically active, healthy young men were randomly divided into three groups that performed: aerobic and resistance training consecutively in the same training session (SS) 2‐3 days wk−1 or on alternating days (AD) 4‐6 days wk−1 as well as control (C). The total training volume was matched in the training groups. The control group was asked to maintain their habitual physical activity and exercise level. Maximal leg press strength (1RM) and peak oxygen uptake (VO2peak) were measured. Abdominal fat mass was estimated with dual‐energy absorptiometry (DXA). High‐sensitivity C‐reactive protein (hs‐CRP), interleukin‐6 (IL‐6), monocyte chemoattractant protein 1 (MCP‐1), tumor necrosis factor alpha (TNF‐α), and adipocytokines resistin, adiponectin, and leptin were analyzed from plasma samples. Training significantly reduced circulating hs‐CRP, leptin, and resistin in both training groups (P<.05), whereas MCP‐1 and TNF‐α decreased only in AD (P<.05). Significant correlations were observed between changes in abdominal fat mass and corresponding changes in MCP‐1, leptin, adiponectin, and resistin. Long‐term combined aerobic and resistance training reduced markers of subclinical inflammation in healthy young men. The results indicate that a higher frequency of individual exercise sessions might be more beneficial with respect to the anti‐inflammatory effects of physical activity. The decreases in inflammation markers seem to be related to decreases in abdominal fat mass.
Six Suggestions for Research on Games in Cognitive Science
Authors: Chabris Christopher F. 
Published: April 2017

AbstractGames are more varied and occupy more of daily life than ever before. At the same time, the tools available to study game play and players are more powerful than ever, especially massive data sets from online platforms and computational engines that can accurately evaluate human decisions. This essay offers six suggestions for future cognitive science research on games: (1) Don't forget about chess, (2) Look beyond action games and chess, (3) Use (near)‐optimal play to understand human play and players, (4) Investigate social phenomena, (5) Raise the standards for studies of games as treatments, (6) Talk to real experts.
Draft Genome Sequence of Uncultured Upland Soil Cluster
Gammaproteobacteria
Gives Molecular Insights into High-Affinity Methanotrophy
Authors: Edwards Collin R. , Onstott Tullis C. , Miller Jennifer M. , Wiggins Jessica B. , Wang Wei , Lee Charles K. , Cary S. Craig , Pointing Stephen B. , Lau Maggie C. Y. 
Published: April 2017

ABSTRACT

Aerated soils form the second largest sink for atmospheric CH
4
. A near-complete genome of uncultured upland soil cluster
Gammaproteobacteria
that oxidize CH
4
at <2.5 ppmv was obtained from incubated Antarctic mineral cryosols. This first genome of high-affinity methanotrophs can help resolve the mysteries about their phylogenetic affiliation and metabolic potential.
Draft Genome Sequence of
Streptomyces
sp. Strain IB2014011-1, Isolated from
Trichoptera
sp. Larvae of Lake Baikal
Authors: Axenov-Gribanov Denis V. , Tokovenko Bogdan T. , Rebets Yuriy V. , Voytsekhovskaya Irina V. , Shatilina Zhanna M. , Protasov Eugenii S. , Luzhetskyy Andriy N. , Timofeyev Maxim A. 
Published: April 2017

ABSTRACT

Unique ecosystems with specific environmental conditions have been proven to be a promising source for isolation of new
Actinobacteria
strains. Ancient Lake Baikal is one of the greatest examples of an ecosystem with high species biodiversity and endemicity caused by long-lasting isolated evolution and stable environmental conditions. Herein we report the draft genome sequence of
Streptomyces
sp. strain IB2014011-1, which was isolated from insect
Trichoptera
sp. larvae collected at the bottom of Lake Baikal.
Complete Coding Sequences of Two Dengue Virus Type 2 Strains Isolated from an Outbreak in Burkina Faso in 2016
Authors: Baronti Cécile , Piorkowski Géraldine , Touret Franck , Charrel Rémi , de Lamballerie Xavier , Nougairede Antoine 
Published: April 2017

ABSTRACT
We report here the complete coding sequences of two strains of dengue virus type 2, isolated in France from patients returning from Burkina Faso in November 2016. Both strains (cosmopolitan genotype) are almost identical (99.91% nucleotide identity) and closely related to a strain circulating in Burkina Faso in 1983.