bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bentonite diminishes DON-induced changes in bone development in mink dams
Authors: Tomaszewska Ewa , Muszyński Siemowit , Dobrowolski Piotr , Kostro Krzysztof , Taszkun Iwona , Żmuda Andrzej , Blicharski Tomasz , Kędzia Paweł 
Published: September 2016

AbstractIntroduction:The aim of this study was to determine the effect of deoxynivalenol (DON), given alone or with bentonite (which eliminates mycotoxicity) in the diet of mink dams throughout mating, pregnancy, and lactation period to pelt harvesting, on the mechanical properties and geometry of their long bones.Material and Methods:The minks were randomly assigned into two groups: a control group (not supplemented with DON, n = 15) and a group fed naturally DON-contaminated wheat and divided into three sub-groups (each sub-group n = 15), depending on bentonite dose: 0 M – sub-group fed naturally DON-contaminated wheat at a concentration of 3.7 mg kg−1alone; 2 M – sub-group fed naturally DON-contaminated wheat at a concentration of 3.7 mg kg−1and bentonite at a concentration of 2 kg 1000 kg−1; 0.5 M – sub-group fed naturally DON-contaminated wheat at a concentration of 3.7 mg kg−1and bentonite at a concentration of 0.5 kg 1000 kg−1.Results:The DON treatment reduced the length of the femur compared to the control group and reduced the bone weight dependently on the amount of bentonite supplementation. However, DON treatment reduced the MRWT and CI of the femur, irrespective of the bentonite supplementation, compared to the control. The total BTD and BMC decreased in all DON-treated groups (irrespective of the bentonite supplementation). Furthermore, the densitometric analysis showed that the main changes in BMD and BMC indicated bone loss in the proximal and distal parts of bone covering the trabecular bone; whereas when bentonite was given at the dose of 2 kg 1000 kg−1an increase in the whole BMD and BMC was observed in the femoral midshaft.Conclusion:Analysis of the geometrical parameters seems to indicate that endosteal resorption was delayed after bentonite supplementation. The addition of bentonite diminished the DON action on bone homeostasis in the mink dams. Thus bentonite could prevent DON-induced bone loss in a dose-dependent manner.
Apoptosis in natural rabies virus infection in dogs
Authors: Vural Sevil Atalay , Bozkurt Mehmet Fatih , Ozkara Ali , Alcigir Mehmet Eray , Ilhan Fatma Sayin 
Published: September 2016

Abstract

Introduction: In the present study apoptosis was investigated in the cornu ammonis and cerebellum of 10 dogs naturally infected with rabies virus. Diagnosis of rabies was based on the results of fluorescent antibody staining and experimental inoculation.

Material and Methods: The paraffin tissue sections were stained with haematoxylin and eosin, avidin-biotin complex peroxidase (ABC-P), and terminal deoxynucleotidyl transferase biotin-dUTP nick end-labelling (TUNEL) methods.

Results: Histopathological examination revealed encephalomyelitis of varying severity and the presence of Negri bodies. Dense rabies antigens were determined in the motor neurons with ABC-P method. On the other hand, Bcl-2 protein and Bax protein gave positive reaction in seven and five cases, respectively. TUNEL staining demonstrated very marked apoptotic changes in the nuclei of neurons localised deep in the substantia alba of the cerebellum. Similar changes were also determined in perivascular mononuclear cells and glia cells within the substantia alba. No apoptopic changes were found in the motor neurons of the cornu ammonis.

Conclusion: The absence of apoptotic changes in the neurons was considered to be the consequence of the necrotic changes that developed in these neurons.
Parapoxvirusorf virus infection induces an increase in interleukin-8, tumour necrosis factor-α, and decorin in goat skin fibroblast cells
Authors: Wang Lingling , Lu Bingzhou , Zheng Haixue , Zhang Keshan , Liu Xiangtao 
Published: September 2016

AbstractIntroduction:Orf virus (ORFV) is a prototypeParapoxvirusspecies in the Poxviridae family that causes serious zoonotic infectious disease. Goat skin fibroblast (GSF) cells are the major host targets of ORFV. Interleukin 8 (IL-8) and tumour necrosis factor (TNF)-α are known to play a vital role in immune response during viral infections. However, the manner of variation over time of their level of expression in GSF cells remains unclear.Material and Methods:In this study, quantitative enzyme-linked immunosorbent assay chips were used to detect changes in the levels of these cytokines expressed and secreted in GSF cells after ORFV infection.Results:Results showed that the expression of IL-8, TNF-α, and decorin was upregulated in the cell lysates, and that secreted decorin and IL-8 were significantly increased in cell supernatant.Conclusion:The results provided possible approaches to elucidation of how ORFV infection initiates host cell immune response.
Intestinal helminths of raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) from the Augustów Primeval Forest (north-eastern Poland)
Authors: Karamon Jacek , Samorek-Pieróg Małgorzata , Moskwa Bożena , Różycki Mirosław , Bilska-Zając Ewa , Zdybel Jolanta , Włodarczyk Magdalena 
Published: September 2016

Abstract

Introduction: The aim of the study was to determine the prevalence of intestinal helminths in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) in the Augustów Primeval Forest (north-eastern Poland), with particular regard to zoonotic parasites.

Material and Methods: Intestines from 53 raccoon dogs and 66 red foxes were examined with the use of sedimentation and counting technique (SCT). Samples of faeces from 51 red foxes and 50 raccoon dogs were examined with the use of flotation method.

Results: Parasitic helminths were found by SCT in 98.5% of red foxes and 96.2% of raccoon dogs. Both species were infected with: Alaria alata (93.9% and 94.3%, respectively), hookworms (68.2% and 83.0%), Apophallus spp. (7.6% and 15.1%), Mesocestoides spp. (57.6% and 24.5%), Taenia spp. (40.9% and 1.9%), and Toxocara/Toxascaris nematodes (33.3% 15.1%). Echinococcus multilocularis was detected only in red foxes (6.1%), but trematodes Echinostomatidae and nematodes Molineus spp. only in raccoon dogs (18.9% and 41.5%, respectively). Additionally, Capillaria spp. eggs were detected by flotation method in 78.4% of foxes and 20.0% of raccoon dogs.

Conclusion: The study showed a very high percentage of red foxes and raccoon dogs infected with intestinal helminths in the Augustów Primeval Forest. Moreover, dangerous zoonotic parasites also were found, which should be taken into consideration in the assessment of infection risk for humans in this region.
Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals
Authors: Denis Edyta , Bielińska Katarzyna , Wieczorek Kinga , Osek Jacek 
Published: September 2016

Abstract

Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic Escherichia coli (VTEC) detection in slaughtered animal carcasses.

Material and Methods: The procedure included an enrichment step, DNA extraction, and two multiplex real-time PCRs. The first PCR detected the invA and hly genes of Salmonella and L. monocytogenes respectively, the second the vtx1, vtx2, and eae genes of VTEC.

Results: The validation of this method resulted in 100% relative sensitivity, specificity, and accuracy as compared to the reference ISO methods. The limit of detection per swab sample was established at 1 cfu for Salmonella and L. monocytogenes and 2 cfu for VTEC. The authors analysed 265 slaughterhouse-collected swabs from cattle, pig, and poultry carcasses. Among 125 from cattle, 51 were positive for VTEC, 29 for Salmonella, and 1 for L. monocytogenes. Among swabs from pig carcasses (n = 95), three, two, and one sample were positive for these pathogens respectively. None of the microorganisms tested for was identified in 45 samples of poultry origin.

Conclusion: The obtained results showed that the method developed can rapidly identify the main bacterial pathogens that may contaminate carcasses of food-producing animals.
Isolation and characterisation of peroxiredoxin 6 promoter from sheep (Ovis aries)
Authors: Liu Nan-Nan , Liu Zeng-Shan , Lu Shi-Ying , Hu Pan , Zhang Ying , Fu Bao-Quan , Li Yan-Song , Zhou Yu , Zhang Yu , Ren Hong-Lin 
Published: September 2016

Abstract

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein and a unique 1-Cys Prdx of the peroxiredoxin family. The expression and regulation of Prdx6 are implicated in numerous physiological and pathological processes.

Material and Methods: Eight stepwise truncated DNA fragments obtained from the 5′-flank region of the Prdx6 gene were prepared and subcloned into the pSEAP2-Enhancer vectors. To investigate the transcriptional activity of the truncated DNA fragments, the recombinant plasmids were transfected into the COS-1 cells and the transcriptional activity was measured via assaying the expression of the reporter gene of the secreted alkaline phosphatase.

Results: A 3.4 kb 5′-upstream flank region of the Prdx6 gene was cloned and sequenced. The region from −108 nt to −36 nt of the 5′-flanking region of the Prdx6 gene contained basal transcriptional activity.

Conclusion: This result provides the basis for further studies on the gene regulation of the Prdx6-mediated biological processes and on screening for the transacting factors that interact with cis-acting elements of the Prdx6 gene promoter.
Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture
Authors: Mazurkevych Anatoliy , Malyuk Mykola , Bezdieniezhnykh Natalia , Starodub Lyubov , Kharkevych Yuriy , Brusko Evgen , Gryzińska Magdalena , Jakubczak Andrzej 
Published: September 2016

Abstract

Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture.

Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study.

Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells.

Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.
Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats
Authors: Mansouri Esrafil , Asadi-Samani Majid , Kooti Wesam , Ghasemiboroon Maryam , Ashtary-Larky Damoon , Alamiri Fatima , Afrisham Reza , Noohi Zahra Hasanzadeh 
Published: September 2016

Abstract

Introduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.

Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.

Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.

Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.
National and Regional Identity in Kaliningrad Oblast from the Cross-border Perspective
Authors: Zieliński Miłosz J. 
Published: September 2016

Abstract
The article analyses the notion of national and regional identity of contemporary inhabitants of Kaliningrad Oblast, as seen by local government officials, NGO activists and researchers living and working in the Polish cities of Elbląg and Gdańsk. For the purposes of the article, nine in-depth interviews were chosen conducted. The article takes into account interviewees’ opinions based on their experience, everyday cooperation with institutions and private contacts with individuals from Kaliningrad Oblast. The main question addressed is to what extent inhabitants of Kaliningrad Oblast are perceived to be different from their compatriots from other regions of Russia in matters constituting national community: narrative of history, tradition, symbols etc. The role of the Local Small Border Traffic Agreement (LBTA) between Poland and Russia, which has served as an important means of facilitating cross-border contacts, was also evaluated. The article demonstrates that despite come regional peculiarities the most prevailing level of identity in Kaliningrad Oblast is that related to the feeling of belonging to the Russian nation.
Magnetite Nanoparticles Prepared By Spark Erosion
Authors: Maiorov M. , Blums E. , Kronkalns G. , Krumina A. , Lubane M. 
Published: September 2016

Abstract
In the present research, we study a possibility of using the electric spark erosion method as an alternative to the method of chemical co-precipitation for preparation of magnetic nanoparticles. Initiation of high frequency electric discharge between coarse iron particles under a layer of distilled water allows obtaining pure magnetite nanoparticles.
Impact of ICTs on Innovation Activities: Indication for selected European countries
Authors: Zoroja Jovana 
Published: September 2016

Abstract
The development and usage of information and communication technologies (ICTs) has particularly increased in the last two decades, while at the same time showing great potential to improve the efficacy of business processes, facilitate and drive innovations, and therefore increase competitiveness. Innovation activities represent an important factor for social and economic change as well as for increasing competitive advantages at both the national and firm levels. This paper focuses on the role that ICTs play in the innovation performance of selected European countries. Using data drawn from the Eurostat and Global Competitiveness Index (2007–2011) and panel regression analysis, research results indicate that ICTs have a significant impact on business innovation activities.
Analyzing Hotel Innovation Behavior in the Balaton Region of Hungary to Identify Best Practices
Authors: Gyurácz-Németh Petra , Horn Nikolett , Friedrich Nóra 
Published: September 2016

Abstract
The goal of the research is to investigate the hotel innovation behavior in one of the most important tourism regions of Hungary. The Lake Balaton Region is a very popular area of the country with quite a short season (from the middle of June to August 20). The region is the second most visited area based on the number of guests and guest nights in Hungary. The topic of innovation plays a growing role in the Hungarian hotel sector as well, because competition is very high and keeping guests and employees can be a great challenge. The study involved both qualitative and quantitative research. The qualitative research aimed to identify the best practices of the Hotel Európa Fit****superior which introduced an “innovation by inspiring” strategy and applies several types of innovation in a highly recommended way. The respondents of the questionnaire included 28 hotel general managers (52% of the 54-person sample). The results showed a strong relationship between the type of the hotel and the number of innovations as well as the type of the hotel and the frequency of innovation.
Assessment of myocardial metabolic rate of glucose by means of Bayesian ICA and Markov Chain Monte Carlo methods in small animal PET imaging
Authors: Berradja Khadidja , Boughanmi Nabil 
Published: September 2016

Abstract
In dynamic cardiac PET FDG studies the assessment of myocardial metabolic rate of glucose (MMRG) requires the knowledge of the blood input function (IF). IF can be obtained by manual or automatic blood sampling and cross calibrated with PET. These procedures are cumbersome, invasive and generate uncertainties. The IF is contaminated by spillover of radioactivity from the adjacent myocardium and this could cause important error in the estimated MMRG. In this study, we show that the IF can be extracted from the images in a rat heart study with 18F-fluorodeoxyglucose (18F-FDG) by means of Independent Component Analysis (ICA) based on Bayesian theory and Markov Chain Monte Carlo (MCMC) sampling method (BICA). Images of the heart from rats were acquired with the Sherbrooke small animal PET scanner. A region of interest (ROI) was drawn around the rat image and decomposed into blood and tissue using BICA. The Statistical study showed that there is a significant difference (p < 0.05) between MMRG obtained with IF extracted by BICA with respect to IF extracted from measured images corrupted with spillover.
Preparation and characterization of poly (amide-ester-imide)/Na+-MMT nanocomposite via ultrasonic method
Authors: Mirsafaei Razieh , Kolahdoozan Majid 
Published: September 2016

Abstract
The novel poly (amide-ester-imide) (PAEI)/Na+-MMT (montmorillonite) nanocomposites were synthesized by ultrasound-assisted technique successfully. PAEI/Na+-MMT nanocomposites with various content of Na+-MMT were applied to enhance the dispersion of the clay layers with polymer matrix. Water-soluble PAEI containing poly (ethylene glycol) (PEG-6000) was prepared, and nanocomposites with various content of Na+-MMT (5, 10, 15, and 20 mass %) were fabricated by ultrasonic method to produce nano-scale composites. The effects of Na+-MMT on the nanocomposites were investigated by Fourier transform infrared, X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) and for thermal properties. XRD approved the structure information of the synthesized nanomaterials, and it was shown that the interlayer spacing increased with clay loading. The magnitude of dispersion of the clay in the nanocomposite matrix was investigated by TEM and SEM images. The results indicated that the lateral dimension of Na+-MMT particles in composites was approximately 100 nm, and the nanomaterials are well dispersed in the polymer matrix to produce exfoliated structure in polymer/nanocomposite. Thermogravimetric analysis confirms that PAEI/Na+-MMT nanocomposites show superior thermal stability with respect to the pure PAEIs.
Surface complexation modeling of U(VI) sorption on GMZ bentonite in the presence of fulvic acid
Authors: Zheng Jie , Luo Daojun , Qiao Yahua , Wang Liang , Wu Wangsuo , Zhang Chunming , Ye Yuanlv 
Published: September 2016

Abstract
In this work, experiments and modeling for the interactions between uranyl ion and GMZ bentonite in the presence of fulvic acid are presented. The results demonstrated that FA is strongly bound to GMZ bentonite, and these molecules have a very large effect on the U(VI) sorption. The results also demonstrated that U(VI) sorption to GMZ bentonite in the presence and absence of sorbed FA can be well predicted by combining SHM and DLM. According to the model calculations, the nature of the interactions between FA with U(VI) at GMZ bentonite surface is mainly surface complex. The first attempt to simulate clay interaction with humus by the SHM model.
The Relationship Between Nutrition Knowledge and Development of Complications in Type 2 Diabetic Patients
Authors: Korkut Deniz , Ergor Gul , Kaner Gulsah , Kurklu Nilgun Seremet 
Published: September 2016

Abstract Background and Aims: Diabetes is increasing rapidly in Turkey as most countries in the world. The prevention of complications which is the main aim in the treatment of diabetes can be accomplished partly with nutrition education. The aim of this study was to assess the relationship between nutrition knowledge (NK) and complications in patients with type 2 diabetes. Materials and Methods: 280 patients with 8-20 years of diabetes duration who applied to diet outpatient clinic were recruited. The questionnaire was prepared by the investigators to assess the NK. A score was calculated on the scale of 100. The complications were determined based on hospital records and patients’ selfreport. Results: 63.2% of participants were female and 36.8% were male. The most common complications in participants were retinopathy (56.1%) and neuropathy (42.9%). The mean NK score was 80.2±11.7. At least one complication was seen in 85.0% of the participants. There was no significant difference for having any complication in patients with adequate and inadequate NK. However the risk of diabetic foot, and coronary artery disease was significantly higher in women with inadequate NK. Conclusion: NK is quite high in long term diabetic patients. However no effect of the NK on the development of complications could be shown. The difference of effect between men and women could be due to the fact that food is mostly prepared by women thus not much chance of the knowledge of men to be reflected on his eating habits. The knowledge difference among female patients was seen in the results as; in women with inadequate NK, the prevalence of diabetic foot and CAD was significantly higher.
A-voiding representation:Eräugnisand inscription in Celan
Authors: Buhanan Kurt 
Published: September 2016

AbstractThis essay deals with the poetry of Paul Celan, particularly focusing on the event of appearing of the negative as that which a-voids representation. The key term here is “Eräugnis,” a term with important resonances between Celan’s poetry and the thought of Alain Badiou, whose evental philosophy centers on the void. Another immediate point of reference is Martin Heidegger, whose thought and language permeates Celan’s work, and it is likely from Heidegger that Celan takes his impetus in thinking through the problem of representing the void. In readings of poems like “Heute und Morgen,” “Welchen der Steine du hebst,” and “Mandorla,” I argue that Celan is grappling with the appearing of the negative in its function as foundational figure for representation. Celan tropes the negative in his poetry, and this essay explores the theoretical and philosophical implications of this negative visuality.