bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alpha‐thalassaemia promotes frequent vaso‐occlusive crises in children with sickle cell anaemia through haemorheological changes
Authors: Renoux Céline , Connes Philippe , Nader Elie , Skinner Sarah , Faes Camille , Petras Marie , Bertrand Yves , Garnier Nathalie , Cuzzubbo Daniela , Divialle‐Doumdo Lydia , Kebaïli Kamila , Renard Cécile , Gauthier Alexandra , Etienne‐Julan Maryse , Cannas Giovanna , Martin Cyril , Hardy‐Dessources Marie‐Dominique , Pialoux Vincent , Romana Marc , Joly Philippe 
Published: January 2017

AbstractBackgroundSickle cell anaemia (SCA) is a severe hereditary haemoglobinopathy characterised by haemorheological abnormalities, which play a role in the occurrence of several acute and chronic clinical complications. While βS‐haplotypes and alpha‐thalassaemia modulate SCA clinical severity, their effects on blood rheology have been incompletely described. The aim of this study was to test the effects of these genetic modifiers on the haemorheological properties and clinical complication of children with SCA.ProcedureSteady‐state haemorheological profile, biological parameters, βS‐haplotypes, alpha‐globin status, vaso‐occlusive crisis (VOC) and acute chest syndrome frequencies were analysed in 128 children (aged 5 to 18 years) with SCA.ResultsPatients with alpha‐thalassaemia showed increased red blood cell (RBC) deformability and aggregation compared to those without. Median VOC rate was higher in patients with homozygous alpha‐thalassaemia compared to those with a normal alpha genotype. Conversely, the haemorheological profile and clinical complications were not influenced by the βS‐haplotypes in our study.ConclusionOur results demonstrate that alpha‐thalassaemia is associated with higher risk for VOC events in children with SCA, which may be due in part to its effects on RBC deformability and aggregation.
Exposure to reactive intermediate‐inducing drugs during pregnancy and the incident use of psychotropic medications among children
Authors: Tran Yen‐Hao , Groen Henk , Bergman Jorieke E.H. , Hak Eelko , Wilffert Bob 
Published: January 2017

AbstractPurposeOur study aimed to investigate the association between prenatal exposure to reactive intermediate (RI)‐inducing drugs and the initiation of psychotropic medications among children.MethodsWe designed a cohort study using a pharmacy prescription database. Pregnant women were considered exposed when they received a prescription of RI‐inducing drugs. These drugs could be either used alone (RI+/FAA−) or combined with drugs exhibiting folic acid antagonism (FAA, RI+/FAA+). The reference group included pregnant women who did not receive any RI‐inducing drugs or FAA drugs.ResultsWe analyzed 4116 exposed and 30 422 reference pregnancies. The hazard ratio (HR) with 95% confidence interval (CI) was 1.27 (95%CI 1.15–1.41) for pregnancies exposed to RI‐inducing drugs as a whole. Considering subgroups of RI‐inducing drugs, prenatal exposure to both RI+/FAA+ and RI+/FAA− was associated with the children's initiation of psychotropic medications, HRs being 1.35 (95%CI 1.10–1.66) and 1.26 (1.13–1.41), respectively. The HRs were increased with prolonged exposure to RI‐inducing drugs, especially in the first and second trimesters. In a detailed examination of the psychotropics, the incidences of receiving antimigraine preparations and psychostimulants were significantly increased for the exposed children, compared with the reference children. The incidences of receiving antipsychotics and hypnotics were also higher for the exposed children; however, the HRs did not reach significance after adjustment.ConclusionsWe found a significantly increased incident use of psychotropic medications among children prenatally exposed to RI‐inducing drugs, especially during the first and second trimesters. This suggests a detrimental effect during critical periods of brain development. Copyright © 2017 John Wiley & Sons, Ltd.
Evaluating the economic viability of CdTe/CIS and CIGS/CIS tandem photovoltaic modules
Authors: Nanayakkara Sanjini U. , Horowitz Kelsey , Kanevce Ana , Woodhouse Michael , Basore Paul 
Published: January 2017

AbstractWe analyze the potential cost competitiveness of two frameless, glass–glass thin‐film tandem photovoltaic module structures, cadmium telluride (CdTe)/CuInSe2 (CIS) and CuIn0.3Ga0.7Se2 (CIGS)/CIS, based on the demonstrated cost of manufacturing the respective component cell technologies in high volume. To consider multiple economic scenarios, we base the CdTe/CIS module efficiency on the current industrial production of CdTe modules, while for CIGS/CIS, we use an aspirational estimate for CIGS efficiency. We focus on four‐terminal mechanically stacked structures, thus avoiding the need to achieve current matching between the two cells. The top cell in such a tandem must have a transparent back contact, which has not been successfully implemented to date. However, for the purpose of understanding the economic viability of both tandems, we assume that this can be implemented at a cost similar to that of sputtered indium tin oxide. The cost of both tandem module structures was found to be nearly identical on an equal‐area basis and approximately $30/m2 higher than the single‐junction alternatives. Both tandem modules are about 4% (absolute) more efficient than a module by using the top‐cell material alone. We find that these tandem modules might reduce total system cost by as much as 11% in applications having a high area‐related balance‐of‐system cost, such as area‐constrained residential systems; however, the relative advantage of tandems decreases in the cases where balance‐of‐system costs are lower, such as in commercial and utility scale systems. Copyright © 2017 John Wiley & Sons, Ltd.
Structural and functional insights into thermally stable cytochrome c′ from a thermophile
Authors: Fujii Sotaro , Oki Hiroya , Kawahara Kazuki , Yamane Daisuke , Yamanaka Masaru , Maruno Takahiro , Kobayashi Yuji , Masanari Misa , Wakai Satoshi , Nishihara Hirofumi , Ohkubo Tadayasu , Sambongi Yoshihiro 
Published: January 2017

AbstractThermophilic Hydrogenophilus thermoluteolus cytochrome c′ (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c′ (AVCP), which has a homo‐dimeric structure and ligand‐binding ability. To understand the thermal stability mechanism and ligand‐binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo‐dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 Å. In the PHCP structure, six specific residues appeared to strengthen the heme‐related and subunit–subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit–subunit interactions were more severely destabilized than ones having altered heme‐related interactions. The PHCP structure further revealed a ligand‐binding channel and a penta‐coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme‐related and subunit–subunit interactions with conservation of the ligand‐binding ability.Brief SummaryWe report the X‐ray crystal structure of cytochrome c′ (PHCP) from thermophilic Hydrogenophilus thermoluteolus. The high thermal stability of PHCP was attributed to heme‐related and subunit–subunit interactions, which were confirmed by a mutagenesis study. The ligand‐binding ability of PHCP was examined by spectrophotometry. PHCP acquired the thermal stability with conservation of the ligand‐binding ability. This study furthers the understanding of the stability and function of cytochromes c.
Comparative evaluation of label‐free quantification methods for shotgun proteomics
Authors: Bubis Julia A. , Levitsky Lev I. , Ivanov Mark V. , Tarasova Irina A. , Gorshkov Mikhail V. 
Published: January 2017

RationaleLabel‐free quantification (LFQ) is a popular strategy for shotgun proteomics. A variety of LFQ algorithms have been developed recently. However, a comprehensive comparison of the most commonly used LFQ methods is still rare, in part due to a lack of clear metrics for their evaluation and an annotated and quantitatively well‐characterized data set.MethodsFive LFQ methods were compared: spectral counting based algorithms SIN, emPAI, and NSAF, and approaches relying on the extracted ion chromatogram (XIC) intensities, MaxLFQ and Quanti. We used three criteria for performance evaluation: coefficient of variation (CV) of protein abundances between replicates; analysis of variance (ANOVA); and the root‐mean‐square error of logarithmized calculated concentration ratios, referred to as standard quantification error (SQE). Comparison was performed using a quantitatively annotated publicly available data set.ResultsThe best results in terms of inter‐replicate reproducibility were observed for MaxLFQ and NSAF, although they exhibited larger standard quantification errors. Using NSAF, all quantitatively annotated proteins were correctly identified in the Bonferronni‐corrected results of the ANOVA test. SIN was found to be the most accurate in terms of SQE. Finally, the current implementations of XIC‐based LFQ methods did not outperform the methods based on spectral counting for the data set used in this study.ConclusionsSurprisingly, the performances of XIC‐based approaches measured using three independent metrics were found to be comparable with more straightforward and simple MS/MS‐based spectral counting approaches. The study revealed no clear leader among the latter. Copyright © 2017 John Wiley & Sons, Ltd.
ESIPT Coupled RAHB Probe for Estimation of Cyanide in Human Blood Serum/Plasma like Solutions Using Chemodosimetric Approach
Authors: Gupta Akul Sen , Paul Kamaldeep , Luxami Vijay 
Published: January 2017

AbstractBenzimidazole based probe 2 has been synthesized that exhibits both RAHBs and ESIPT phenomenons on single platform. Probe 2 is used for selective estimation of CN− ions. The chemodosimetric approach is successfully used for estimation of CN− ions (100 nM) in human blood sample and plasma like solutions. The interaction behaviour of CN− with probe 2 has been confirmed through NMR titrations as well as DFT calculations.
Structural, Textural, and Catalytic Properties of Ti(IV)‐Fe(III) Mixed Oxides Prepared by a Modified Sol‐Gel Route
Authors: Khaleel Abbas , Al‐Zuhair Sulaiman , Al‐Mamary Said , Parvin Maliha , Khan Ahmad H. 
Published: January 2017

AbstractIron(III) titanates and Fe(III)‐modified titania were prepared via a modified sol‐gel method. Structures, texture and phase transformations after calcination at different temperatures were investigated. Fe(III) ions below 10 % were well dispersed in the titania lattice. Pseudorutile (Fe2Ti3O9) was prepared starting with a solution containing 40 % Fe(III) and calcining the product at 500 °C. Starting with equal concentrations of both metal ions resulted in a mixture of pseudorutile and pseudobrookite, Fe2TiO5. However, using 66.7 % Fe(III) to prepare pseudobrookite resulted in segregation of trace amounts of iron(III) oxide and rutile. All mixed oxides possessed significantly higher surface areas (178‐217 m2/g), larger pore volumes (0.16‐0.27 cc/g), and more homogeneous mesopores compared with the parent single metal oxides. Fe2TiO5 showed promising catalytic activity in the catalytic oxidation of toluene. A conversion of 100 % to CO2 was obtained at temperatures ≥300 °C and its catalytic activity correlates with its enhanced reducibility as studied by H2‐temperature programmed reduction (H2‐TPR).
Title: Periodic Mesoporous Hexagonal Boron Nitride at High Pressure: A Route to Cubic Boron Nitride Nanocrystals and Mesoporous Cubic Boron Nitride
Authors: Mandal Manik , Liu Cong , Sanders Toby , Haso Fadi , Bhadram Venkata , Arslan Ilke , Liu Tianbo , Fei Yingwei , Landskron Kai 
Published: January 2017

AbstractPeriodic mesoporous hexagonal boron nitride was investigated as a precursor for nanoscale forms of cubic boron nitride. Cubic boron nitride (c‐BN) nanocrystals were obtained at a pressure of 14 GPa and a temperature of 1300 °C. The synthesized nanocrystals have diameters of ∼ 50 nm and are solution‐processible. Mesoporous c‐BN formed at a pressure of 10 GPa and 1000 °C. The mesoporous c‐BN has a pore size distribution centered around 3 nm and a surface area of 122 m2/g as determined by electron tomography. At pressures of 8 and 6 GPa near monodisperse h‐BN nanodiscs with diameters of 0.5‐1 μm and 0.2‐0.5 μm were formed, respectively. The discs formed colloidal solutions in acetone. The hydrodynamic radius of the discs matched the radii determined by dynamic light scattering indicating absence of nanodisc aggregation in solution.
Quickly Manufactured, Drug Eluting, Calcium Phosphate Composite Coating
Authors: Thomas Midhun Ben , Metoki Noah , Geuli Ori , Sharabani‐Yosef Orna , Zada Tal , Reches Meital , Mandler Daniel , Eliaz Noam 
Published: January 2017

AbstractCalcium phosphate (CaP) ceramics have been prevalently used as coatings for implants because of their excellent osteoconductive and bioactive properties. Yet, bone regeneration procedures might have complications such as bacterial infection, local inflammation, bone destruction, and impaired bone healing. Here, we present a novel in situ electrodeposition of CaP with chitosan nanoparticles containing antibiotics. The deposition was shown to be fast and efficient. The deposited layer of octacalcium phosphate (OCP) and monotite contained a large amount of gentamicin, which was released gradually over a period of 15 days. These phases may be beneficial for bone growth, as OCP has higher solubility than the stoichiometric hydroxyapatite (HAp) and is commonly considered as a precursor to HAp, while monotite has even faster resorbability. In addition, both the cytotoxicity and biomineralization of the coating were studied, and the coating was proven to be non‐cytotoxic and highly biomimetic.
PTSA‐Catalyzed Reaction of Indoles with 2‐Oxoaldehydes: Synthesis of α,α‐Bis(indol‐3‐yl) Ketones
Authors: Suárez Anisley , Martínez Fernando , Suárez‐Pantiga Samuel , Sanz Roberto 
Published: January 2017

AbstractA convenient procedure for accessing α,α‐bis(indol‐3‐yl) ketones from indoles and α‐oxoaldehydes is described using an inexpensive and commercially available catalyst such as p‐toluenesulfonic acid monohydrate. This protocol allows for the first time the synthesis of 2,2‐bis(indolyl)‐1‐alkylethanones by employing aliphatic 2‐oxoaldehydes, even as aqueous solutions. The high‐yielded obtained ketones have been shown as useful starting materials for further synthetic transformations.
Low Dose Radiation Causes Skin Cancer in Mice and Has a Differential Effect on Distinct Epidermal Stem Cells
Authors: Revenco Tatiana , Lapouge Gaelle , Moers Virginie , Brohée Sylvain , Sotiropoulou Panagiota A. 
Published: January 2017

Abstract
The carcinogenic effect of ionizing radiation has been evaluated based on limited populations accidently exposed to high dose radiation. In contrast, insufficient data are available on the effect of low dose radiation (LDR), such as radiation deriving from medical investigations and interventions, as well as occupational exposure that concern a large fraction of western populations. Using mouse skin epidermis as a model, we showed that LDR results in DNA damage in sebaceous gland (SG) and bulge epidermal stem cells (SCs). While the first commit apoptosis upon low dose irradiation, the latter survive. Bulge SC survival coincides with higher HIF-1α expression and a metabolic switch upon LDR. Knocking down HIF-1α sensitizes bulge SCs to LDR-induced apoptosis, while upregulation of HIF-1α in the epidermis, including SG SCs, rescues cell death. Most importantly, we show that LDR results in cancer formation with full penetrance in the radiation-sensitive Patched1 heterozygous mice. Overall, our results demonstrate for the first time that LDR can be a potent carcinogen in individuals predisposed to cancer.
Direct bioethanol production from wheat straw using xylose/glucose co-fermentation by co-culture of two recombinant yeasts
Authors: Zhang Yuanyuan , Wang Caiyun , Wang Lulu , Yang Ruoxin , Hou Peilei , Liu Junhong 
Published: January 2017

Abstract
To achieve a cost-effective bioconversion of lignocellulosic materials, a novel xylose/glucose co-fermentation process by co-culture of cellulose-utilizing recombinant Saccharomyces cerevisiae (S. cerevisiae) and xylan-utilizing recombinant Pichia pastoris (P. pastoris) was developed, in which ethanol was produced directly from wheat straw without additional hydrolytic enzymes. Recombinant S. cerevisiae coexpressing three types of cellulase and recombinant P. pastoris coexpressing two types of xylanase were constructed, respectively. All cellulases and xylanases were successfully expressed and similar extracellular activity was demonstrated. The maximum ethanol concentration of 32.6 g L−1 with the yield 0.42 g g−1 was achieved from wheat straw corresponding to 100 g L−1 of total sugar after 80 h co-fermentation, which corresponds to 82.6% of the theoretical yield. These results demonstrate that the direct and efficient ethanol production from lignocellulosic materials is accomplished by simultaneous saccharification (cellulose and hemicellulose) and co-fermentation (glucose and xylose) with the co-culture of the two recombinant yeasts.
Ketogenic diet in pyruvate dehydrogenase complex deficiency: short‐ and long‐term outcomes
Authors: Sofou Kalliopi , Dahlin Maria , Hallböök Tove , Lindefeldt Marie , Viggedal Gerd , Darin Niklas 
Published: January 2017

AbstractObjectivesOur aime was to study the short‐ and long‐term effects of ketogenic diet on the disease course and disease‐related outcomes in patients with pyruvate dehydrogenase complex deficiency, the metabolic factors implicated in treatment outcomes, and potential safety and compliance issues.MethodsPediatric patients diagnosed with pyruvate dehydrogenase complex deficiency in Sweden and treated with ketogenic diet were evaluated. Study assessments at specific time points included developmental and neurocognitive testing, patient log books, and investigator and parental questionnaires. A systematic literature review was also performed.ResultsNineteen patients were assessed, the majority having prenatal disease onset. Patients were treated with ketogenic diet for a median of 2.9 years. All patients alive at the time of data registration at a median age of 6 years. The treatment had a positive effect mainly in the areas of epilepsy, ataxia, sleep disturbance, speech/language development, social functioning, and frequency of hospitalizations. It was also safe—except in one patient who discontinued because of acute pancreatitis. The median plasma concentration of ketone bodies (3‐hydroxybutyric acid) was 3.3 mmol/l. Poor dietary compliance was associated with relapsing ataxia and stagnation of motor and neurocognitive development. Results of neurocognitive testing are reported for 12 of 19 patients.ConclusionKetogenic diet was an effective and safe treatment for the majority of patients. Treatment effect was mainly determined by disease phenotype and attainment and maintenance of ketosis.
Erectile Dysfunction in Male Adults with Atopic Dermatitis and Psoriasis
Authors: Egeberg Alexander , Hansen Peter R. , Gislason Gunnar H. , Skov Lone , Thyssen Jacob P. 
Published: January 2017

Abstract

Introduction
Patients with psoriasis have increased risk of cardiovascular disease, but data on atopic dermatitis (AD) are less clear-cut. However, it is well-established that erectile dysfunction (ED) can serve as a risk marker for coronary disease.

Aim
To investigate the incidence, prevalence, and risk of ED in men with psoriasis and AD.

Methods
The sample included all Danish men at least 30 years old. In patients with AD and psoriasis, we determined disease severity based on use of systemic therapy. We performed a cross-sectional study (January 1, 2008) using logistic regression to estimate the prevalence and odds ratio of ED. Moreover, in a cohort study design, patients were followed from January 1, 2008 through December 31, 2012, and Cox regression models were used to estimate adjusted hazard ratios of new-onset ED. Models were adjusted for potential confounding factors, including age, socioeconomic status, health care consumption, smoking, alcohol abuse, diabetes, and cholesterol-lowering drug use.

Main Outcome Measures
The outcome was initiation of pharmacotherapy used for treatment of ED.

Results
The sample consisted of 1,756,679 Danish men (age range = 30–100 years), of which 2,373 and 26,536 had adult AD (mild = 1,072; severe = 1,301) and psoriasis (mild = 21,775; severe = 4,761), respectively. Mean ages (SDs) were 53.0 (14.6), 46.7 (12.0), and 56.3 (13.8) years for the general population, patients with AD, and patients with psoriasis, respectively. Prevalences of ED were 8.7%, 6.7%, and 12.8% for the general population, patients with AD, and patients with psoriasis, respectively. Adjusted odds ratios (logistic regression) of ED were decreased in patients with AD (0.68; 0.57–0.80) but increased in those with psoriasis (1.15; 1.11–1.20). Adjusted odds ratios for mild and severe AD were 0.63 (0.48–0.82) and 0.72 (0.58–0.88), respectively, and those for psoriasis these were 1.16 (1.11–1.21) and 1.13 (1.03–1.23). Adjusted hazard ratios (Cox regression) were 0.92 (0.76–1.11) for AD and 1.14 (1.08–1.20) for psoriasis. The ED risk was not increased in men with mild AD (0.85; 0.63–1.14) or severe AD (0.97; 0.76–1.24) but was significantly increased in men with mild psoriasis (1.13; 1.09–1.20) and severe psoriasis (1.17; 1.04–1.32).

Conclusion
We found an increased prevalence and risk of ED in men with psoriasis, whereas the risk was comparable to (and even slightly lower than) the general population for men with AD.
Quantifying non-linearity in planar supersonic potential flows
Authors: Meijer M.-C. , Dala L. , Dala L. 
Published: January 2017

ABSTRACTAn analysis is presented which allows the engineer to quantitatively estimate the validity bounds of aerodynamic methods based in linear potential flows a-priori. The development is limited to quasi-steady planar flows with attached shocks and small body curvature. Perturbation velocities are parameterised in terms of Mach number and flow turning angle by means of a series-expansion for flow velocity based in the method of characteristics. The parameterisation is used to assess the magnitude of non-linear term-groupings relative to linear groups in the full potential equation. This quantification is used to identify dominant nonlinear terms and to estimate the validity of linearising the potential flow equation at a given Mach number and flow turning angle. Example applications include the a-priori estimation of the validity bounds for linear aerodynamic models for supersonic aeroelastic analysis of lifting surfaces and panels.