bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic association of rs1344706 in ZNF804A with bipolar disorder and schizophrenia susceptibility in Chinese populations
Authors: Rao Shuquan , Yao Yao , Ryan Joanne , Jin Chunhui , Xu Yong , Huang Xinhe , Guo Jianxiu , Wen Yueqiang , Mao Canquan , Meyre David , Zhang Fuquan 
Published: January 2017

AbstractRs1344706 in the the zinc finger protein 804A (ZNF804A) gene has been identified to be associated with schizophrenia and bipolar disorder (BD) in Europeans. However, whether rs1344706 is associated with schizophrenia in Chinese populations remains inconclusive; furthermore, the association between rs1344706 and BD in Chinese populations has been rarely explored. To explore the association between rs1344706 and schizophrenia/BD in Chinese populations, we genotyped rs1344706 among 1128 Chinese subjects (537 patients with BD and 591 controls) and found that rs1344706 showed marginal allelic association with BD (P = 0.028) with T-allele being more prevalent in cases than that in controls (OR = 1.19, 95% CI 1.03–1.37). Meta-analysis of rs1344706 by pooling all available data showed that rs1344706 was significantly associated with BD (P = 0.001). Besides, positive association of rs1344706 with schizophrenia was observed in Northern Chinese (P = 0.005). Furthermore, ZNF804A is highly expressed in human and mouse brains, especially in prenatal stage.
Single basal application of thiacloprid for the integrated management of Meloidogyne incognita and Bemisia tabaci in tomato crops
Authors: Dong Sa , Ren Xiaofen , Zhang Dianli , Ji Xiaoxue , Wang Kaiyun , Qiao Kang 
Published: January 2017

AbstractTomato growers commonly face heavy nematode (Meloidogyne incognita) and whitefly (B-biotypeBemisia tabaci) infestations, and previous studies demonstrated that thiacloprid could be used to controlM. incognitaandB. tabaciin cucumber. However, the efficacy of a single basal application of thiacloprid to control both pests and its effect on yield in tomato remains unknown. In this study, the potential of thiacloprid application to the soil for the integrated control ofM. incognitaandB. tabaciin tomato was evaluated in the laboratory and the field. Laboratory tests showed that thiacloprid was highly toxic to whitefly adults and eggs with an average lethal concentration 50 (LC50) of 14.7 and 62.2 mg ai L−1, respectively, and the LC50of thiacloprid for nematode J2s and eggs averaged 36.2 and 70.4 mg ai L−1, respectively. In field trials, when thiacloprid was applied to the soil at 7.5, 15 and 30 kg ha−1in two consecutive years, whitefly adults decreased by 37.8–75.4% within 60 days of treatment, and the root-galling index was reduced by 31.8–85.2%. Optimum tomato plant growth and maximum yields were observed in the 15 kg ha−1treatment. The results indicated that a single basal application of thiacloprid could controlM. incognitaandB. tabaciand enhance tomato growth and yield.
Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines
Authors: Dezest Marlène , Chavatte Laurent , Bourdens Marion , Quinton Damien , Camus Mylène , Garrigues Luc , Descargues Pascal , Arbault Stéphane , Burlet-Schiltz Odile , Casteilla Louis , Clément Franck , Planat Valérie , Bulteau Anne-Laure 
Published: January 2017

AbstractCompelling evidence suggests that Cold Atmospheric Pressure Plasma (CAPP) has potential as a new cancer therapy. However, knowledge about cellular signaling events and toxicity subsequent to plasma treatment is still poorly documented. The aim of this study was to focus on the interaction between 3 different types of plasma (He, He-O2, He-N2) and human epithelial cell lines to gain better insight into plasma-cell interaction. We provide evidence that reactive oxygen and nitrogen species (RONS) are inducing cell death by apoptosis and that the proteasome, a major intracellular proteolytic system which is important for tumor cell growth and survival, is a target of (He or He-N2) CAPP. However, RONS are not the only actors involved in cell death; electric field and charged particles could play a significant role especially for He-O2 CAPP. By differential label-free quantitative proteomic analysis we found that CAPP triggers antioxidant and cellular defense but is also affecting extracellular matrix in keratinocytes. Moreover, we found that malignant cells are more resistant to CAPP treatment than normal cells. Taken together, our findings provide insight into potential mechanisms of CAPP-induced proteasome inactivation and the cellular consequences of these events.
Targeted metabolomics shows plasticity in the evolution of signaling lipids and uncovers old and new endocannabinoids in the plant kingdom
Authors: Gachet María Salomé , Schubert Alexandra , Calarco Serafina , Boccard Julien , Gertsch Jürg 
Published: January 2017

AbstractThe remarkable absence of arachidonic acid (AA) in seed plants prompted us to systematically study the presence of C20 polyunsaturated fatty acids, stearic acid, oleic acid, jasmonic acid (JA), N-acylethanolamines (NAEs) and endocannabinoids (ECs) in 71 plant species representative of major phylogenetic clades. Given the difficulty of extrapolating information about lipid metabolites from genetic data we employed targeted metabolomics using LC-MS/MS and GC-MS to study these signaling lipids in plant evolution. Intriguingly, the distribution of AA among the clades showed an inverse correlation with JA which was less present in algae, bryophytes and monilophytes. Conversely, ECs co-occurred with AA in algae and in the lower plants (bryophytes and monilophytes), thus prior to the evolution of cannabinoid receptors in Animalia. We identified two novel EC-like molecules derived from the eicosatetraenoic acid juniperonic acid, an omega-3 structural isomer of AA, namely juniperoyl ethanolamide and 2-juniperoyl glycerol in gymnosperms, lycophytes and few monilophytes. Principal component analysis of the targeted metabolic profiles suggested that distinct NAEs may occur in different monophyletic taxa. This is the first report on the molecular phylogenetic distribution of apparently ancient lipids in the plant kingdom, indicating biosynthetic plasticity and potential physiological roles of EC-like lipids in plants.
Clusterin: full-length protein and one of its chains show opposing effects on cellular lipid accumulation
Authors: Matukumalli Suvarsha Rao , Tangirala Ramakrishna , Rao C. M. 
Published: January 2017

AbstractProteins, made up of either single or multiple chains, are designed to carry out specific biological functions. We found an interesting example of a two-chain protein where administration of one of its chains leads to a diametrically opposite outcome than that reported for the full-length protein. Clusterin is a highly glycosylated protein consisting of two chains, α- and β-clusterin. We have investigated the conformational features, cellular localization, lipid accumulation, in vivo effects and histological changes upon administration of recombinant individual chains of clusterin. We demonstrate that recombinant α- and β-chains exhibit structural and functional differences and differ in their sub-cellular localization. Full-length clusterin is known to lower lipid levels. In contrast, we find that β-chain-treated cells accumulate 2-fold more lipid than controls. Interestingly, α-chain-treated cells do not show such increase. Rabbits injected with β-chain, but not α-chain, show ~40% increase in weight, with adipocyte hypertrophy, liver and kidney steatosis. Many, sometimes contrasting, roles are ascribed to clusterin in obesity, metabolic syndrome and related conditions. Our findings of differential localization and activities of individual chains of clusterin should help in understanding better the roles of clusterin in metabolism.
Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity
Authors: Choudhury Yukti , Toh Yi Chin , Xing Jiangwa , Qu Yinghua , Poh Jonathan , Li Huan , Tan Hui Shan , Kanesvaran Ravindran , Yu Hanry , Tan Min-Han 
Published: January 2017

AbstractIdiosyncratic drug-induced hepatotoxicity is a major cause of liver damage and drug pipeline failure, and is difficult to study as patient-specific features are not readily incorporated in traditional hepatotoxicity testing approaches using population pooled cell sources. Here we demonstrate the use of patient-specific hepatocyte-like cells (HLCs) derived from induced pluripotent stem cells for modeling idiosyncratic hepatotoxicity to pazopanib (PZ), a tyrosine kinase inhibitor drug associated with significant hepatotoxicity of unknown mechanistic basis. In vitro cytotoxicity assays confirmed that HLCs from patients with clinically identified hepatotoxicity were more sensitive to PZ-induced toxicity than other individuals, while a prototype hepatotoxin acetaminophen was similarly toxic to all HLCs studied. Transcriptional analyses showed that PZ induces oxidative stress (OS) in HLCs in general, but in HLCs from susceptible individuals, PZ causes relative disruption of iron metabolism and higher burden of OS. Our study establishes the first patient-specific HLC-based platform for idiosyncratic hepatotoxicity testing, incorporating multiple potential causative factors and permitting the correlation of transcriptomic and cellular responses to clinical phenotypes. Establishment of patient-specific HLCs with clinical phenotypes representing population variations will be valuable for pharmaceutical drug testing.
Systems biology analysis of drivers underlying hallmarks of cancer cell metabolism
Authors: Zielinski Daniel C. , Jamshidi Neema , Corbett Austin J. , Bordbar Aarash , Thomas Alex , Palsson Bernhard O. 
Published: January 2017

AbstractMalignant transformation is often accompanied by significant metabolic changes. To identify drivers underlying these changes, we calculated metabolic flux states for the NCI60 cell line collection and correlated the variance between metabolic states of these lines with their other properties. The analysis revealed a remarkably consistent structure underlying high flux metabolism. The three primary uptake pathways, glucose, glutamine and serine, are each characterized by three features: (1) metabolite uptake sufficient for the stoichiometric requirement to sustain observed growth, (2) overflow metabolism, which scales with excess nutrient uptake over the basal growth requirement, and (3) redox production, which also scales with nutrient uptake but greatly exceeds the requirement for growth. We discovered that resistance to chemotherapeutic drugs in these lines broadly correlates with the amount of glucose uptake. These results support an interpretation of the Warburg effect and glutamine addiction as features of a growth state that provides resistance to metabolic stress through excess redox and energy production. Furthermore, overflow metabolism observed may indicate that mitochondrial catabolic capacity is a key constraint setting an upper limit on the rate of cofactor production possible. These results provide a greater context within which the metabolic alterations in cancer can be understood.
Extent of Night Warming and Spatially Heterogeneous Cloudiness Differentiate Temporal Trend of Greenness in Mountainous Tropics in the New Century
Authors: Yu Mei , Gao Qiong , Gao Chunxiao , Wang Chao 
Published: January 2017

AbstractTropical forests have essential functions in global C dynamics but vulnerable to changes in land cover land use (LCLUC) and climate. The tropics of Caribbean are experiencing warming and drying climate and diverse LCLUC. However, large-scale studies to detect long-term trends of C and mechanisms behind are still rare. Using MODIS Enhanced Vegetation Index (EVI), we investigated greenness trend in the Greater Antilles Caribbean during 2000–2015, and analyzed trend of vegetation patches without LCLUC to give prominence to climate impacts. We hypothesized that night warming and heavy cloudiness would reduce EVI in this mountainous tropical region. Over the 15 years, EVI decreased significantly in Jamaica, Haiti, Dominican Republic, and Puerto Rico, but increased in Cuba partly due to its strong reforestation. Haiti had the largest decreasing trend because of continuous deforestation for charcoals. After LCLUC was excluded, EVI trend still varied greatly, decreasing in the windward but increasing in the leeward of Puerto Rico. Nighttime warming reinforced by spatially heterogeneous cloudiness was found to significantly and negatively correlate with EVI trend, and explained the spatial pattern of the latter. Although cooled daytime and increased rainfall might enhance EVI, nighttime warming dominated the climate impacts and differentiated the EVI trend.
Genomic structural variations for cardiovascular and metabolic comorbidity
Authors: Nazarenko Maria S. , Sleptcov Aleksei A. , Lebedev Igor N. , Skryabin Nikolay A. , Markov Anton V. , Golubenko Maria V. , Koroleva Iuliia A. , Kazancev Anton N. , Barbarash Olga L. , Puzyrev Valery P. 
Published: January 2017

AbstractThe objective of this study was to identify genes targeted by both copy number and copy-neutral changes in the right coronary arteries in the area of advanced atherosclerotic plaques and intact internal mammary arteries derived from the same individuals with comorbid coronary artery disease and metabolic syndrome. The artery samples from 10 patients were screened for genomic imbalances using array comparative genomic hybridization. Ninety high-confidence, identical copy number variations (CNVs) were detected. We also identified eight copy-neutral changes (cn-LOHs) > 1.5 Mb in paired arterial samples in 4 of 10 individuals. The frequencies of the two gains located in the 10q24.31 (ERLIN1) and 12q24.11 (UNG, ACACB) genomic regions were evaluated in 33 paired arteries and blood samples. Two patients contained the gain in 10q24.31 (ERLIN1) and one patient contained the gain in 12q24.11 (UNG, ACACB) that affected only the blood DNA. An additional two patients harboured these CNVs in both the arteries and blood. In conclusion, we discovered and confirmed a gain of the 10q24.31 (ERLIN1) and 12q24.11 (UNG, ACACB) genomic regions in patients with coronary artery disease and metabolic comorbidity. Analysis of DNA extracted from blood indicated a possible somatic origin for these CNVs.
Dynamical proxies of North Atlantic predictability and extremes
Authors: Faranda Davide , Messori Gabriele , Yiou Pascal 
Published: January 2017

AbstractAtmospheric flows are characterized by chaotic dynamics and recurring large-scale patterns. These two characteristics point to the existence of an atmospheric attractor defined by Lorenz as: “the collection of all states that the system can assume or approach again and again, as opposed to those that it will ultimately avoid”. The average dimension D of the attractor corresponds to the number of degrees of freedom sufficient to describe the atmospheric circulation. However, obtaining reliable estimates of D has proved challenging. Moreover, D does not provide information on transient atmospheric motions, such as those leading to weather extremes. Using recent developments in dynamical systems theory, we show that such motions can be classified through instantaneous rather than average properties of the attractor. The instantaneous properties are uniquely determined by instantaneous dimension and stability. Their extreme values correspond to specific atmospheric patterns, and match extreme weather occurrences. We further show the existence of a significant correlation between the time series of instantaneous stability and dimension and the mean spread of sea-level pressure fields in an operational ensemble weather forecast at lead times of over two weeks. Instantaneous properties of the attractor therefore provide an efficient way of evaluating and informing operational weather forecasts.
Regioselectivity and Mechanism of Synthesizing N-Substituted 2-Pyridones and 2-Substituted Pyridines via Metal-Free C-O and C-N Bond-Cleaving of Oxazoline[3,2-a]pyridiniums
Authors: Li Bo , Xue Susu , Yang Yang , Feng Jia , Liu Peng , Zhang Yong , Zhu Jianming , Xu Zhijian , Hall Adrian , Zhao Bo , Shi Jiye , Zhu Weiliang 
Published: January 2017

AbstractNovel intermediate oxazoline[3,2-a]pyridiniums were facilely prepared from 2-(2,2-dimethoxyethoxy)-pyridines via acid promoted intramolecular cyclization. Sequentially, the quaternary ammonium salts were treated with different nucleophiles for performing regioselective metal-free C-O and C-N bond-cleaving to afford prevalent heterocyclic structures of N-substituted pyridones and 2-substituted pyridines. The reaction mechanism and regioselectivity were then systematically explored by quantum chemistry calculations at B3LYP/6-31 g(d) level. The calculated free energy barrier of the reactions revealed that aniline and aliphatic amines (e.g., methylamine) prefer to attack C8 of intermediate 4a, affording N-substituted pyridones, while phenylmethanamine, 2-phenylethan-1-amine and 3-phenylpropan-1-amine favor to attack C2 of the intermediate to form 2-substituted pyridines. With the optimized geometries of the transition states, we found that the aromatic ring of the phenyl aliphatic amines may form cation-π interaction with the pyridinium of the intermediates, which could stabilize the transition states and facilitate the formation of 2-substituted pyridines.
ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain
Authors: Cabukusta Birol , Kol Matthijs , Kneller Laura , Hilderink Angelika , Bickert Andreas , Mina John G. M. , Korneev Sergei , Holthuis Joost C. M. 
Published: January 2017

AbstractSMSr/SAMD8 is an ER-resident ceramide phosphoethanolamine synthase with a critical role in controlling ER ceramides and suppressing ceramide-induced apoptosis in cultured cells. SMSr-mediated ceramide homeostasis relies on the enzyme’s catalytic activity as well as on its N-terminal sterile α-motif or SAM domain. Here we report that SMSr-SAM is structurally and functionally related to the SAM domain of diacylglycerol kinase DGKδ, a central regulator of lipid signaling at the plasma membrane. Native gel electrophoresis indicates that both SAM domains form homotypic oligomers. Chemical crosslinking studies show that SMSr self-associates into ER-resident trimers and hexamers that resemble the helical oligomers formed by DGKδ-SAM. Residues critical for DGKδ-SAM oligomerization are conserved in SMSr-SAM and their substitution causes a dissociation of SMSr oligomers as well as a partial redistribution of the enzyme to the Golgi. Conversely, treatment of cells with curcumin, a drug disrupting ceramide and Ca2+ homeostasis in the ER, stabilizes SMSr oligomers and promotes retention of the enzyme in the ER. Our data provide first demonstration of a multi-pass membrane protein that undergoes homotypic oligomerization via its SAM domain and indicate that SAM-mediated self-assembly of SMSr is required for efficient retention of the enzyme in the ER.
Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Authors: Omatsu-Kanbe Mariko , Nozuchi Nozomi , Nishino Yuka , Mukaisho Ken-ichi , Sugihara Hiroyuki , Matsuura Hiroshi 
Published: January 2017

AbstractAtypically-shaped cardiomyocytes (ACMs) are beating heart cells identified in the cultures of cardiomyocyte-removed fractions obtained from adult mouse hearts. Since ACMs spontaneously develop into beating cells in the absence of hormones or chemicals, these cells are likely to be a type of cardiac progenitors rather than stem cells. “Native ACMs” are found as small interstitial cells among ventricular myocytes that co-express cellular prion protein (PrP) and cardiac troponin T (cTnT) in mouse and human heart tissues. However, the endogenous behavior of human ACMs is unclear. In the present study, we demonstrate that PrP+ cTnT+ cells are present in the human heart tissue with myocardial infarction (MI). These cells were mainly found in the border of necrotic cardiomyocytes caused by infarcts and also in the hibernating myocardium subjected to the chronic ischemia. The ratio of PrP+ cTnT+ cells to the total cells observed in the normal heart tissue section of mouse and human was estimated to range from 0.3–0.8%. Notably, living human PrP+ cTnT+ cells were identified in the cultures obtained at pathological autopsy despite exposure to lethal ischemic conditions for hours after death. These findings suggest that ACMs could survive in the ischemic human heart and develop into a sub-population of cardiac myocytes.
Six-band terahertz metamaterial absorber based on the combination of multiple-order responses of metallic patches in a dual-layer stacked resonance structure
Authors: Wang Ben-Xin , Wang Gui-Zhen , Sang Tian , Wang Ling-Ling 
Published: January 2017

AbstractThis paper reports on a numerical study of the six-band metamaterial absorber composed of two alternating stack of metallic-dielectric layers on top of a continuous metallic plane. Six obvious resonance peaks with high absorption performance (average larger than 99.37%) are realized. The first, third, fifth, and the second, fourth, sixth resonance absorption bands are attributed to the multiple-order responses (i.e., the 1-, 3- and 5-order responses) of the bottom- and top-layer of the structure, respectively, and thus the absorption mechanism of six-band absorber is due to the combination of two sets of the multiple-order resonances of these two layers. Besides, the size changes of the metallic layers have the ability to tune the frequencies of the six-band absorber. Employing the results, we also present a six-band polarization tunable absorber through varying the sizes of the structure in two orthogonal polarization directions. Moreover, nine-band terahertz absorber can be achieved by using a three-layer stacked structure. Simulation results indicate that the absorber possesses nine distinct resonance bands, and average absorptivities of them are larger than 94.03%. The six-band or nine-band absorbers obtained here have potential applications in many optoelectronic and engineering technology areas.
Spatial distribution of electrons near the Fermi level in the metallic LaB6 through accurate X-ray charge density study
Authors: Kasai Hidetaka , Nishibori Eiji 
Published: January 2017

AbstractCharge densities of iso-structural metal hexaborides, a transparent metal LaB6 and a semiconductor BaB6, have been determined using the d > 0.22 Å ultra-high resolution synchrotron radiation X-ray diffraction data by a multipole refinement and a maximum entropy method (MEM). The quality of the experimental charge densities was evaluated by comparison with theoretical charge densities. The strong inter-octahedral and relatively weak intra-octahedral boron-boron bonds were observed in the charge densities. A difference of valence charge densities between LaB6 and BaB6 was calculated to reveal a small difference between isostructural metal and semiconductor. The weak electron lobes distributed around the inter B6 octahedral bond were observed in the difference density. We found the electron lobes are the conductive π-electrons in LaB6 from the comparison with the theoretical valence charge density. We successfully observed a spatial distribution of electrons near the Fermi level from the X-ray charge density study of the series of iso-structural solids.