bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of orthodontic treatment with aligners on the oral health‐related quality of life of patients with trisomy 21
Authors: Taniguchi André , Bittencourt Jéssica Madeira , Paiva Saul Martins , Fernandes Thais Maria Freire , Almeida Marcio Rodriguez de , Almeida‐Pedrin Renata Rodrigues de , Conti Ana Cláudia de Castro Ferreira , Bespalez‐Neto Roberto , Pedron Oltramari Paula Vanessa 
Published: September 2024

AbstractObjectiveTo assess the impact of treatment with orthodontic aligners (OA) on oral health‐related quality of life (OHRQoL) in patients with trisomy 21 (T21) compared to non‐syndromic patients.Materials and methodsThe sample consisted of 30 patients, both sexes, aged between 11 and 35 years, divided into two groups: T21 (n = 10, patients with T21, treated prospectively) and CONTROL (n = 20, control group, non‐syndromic patients, from the Orthodontic Laboratory of UNOPAR). In both groups, patients were treated with Invisalign orthodontic aligners (Align Technology), following the same treatment parameters. Participants, assisted by their caregivers, when necessary, answered Oral Health Impact Profile (OHIP‐14) instrument and the patient's guardians answered the Oral Health Scale for People with Down's syndrome (OHDS) instrument, before (T0) and after 30 (T1), 180 (T2), and 365 (T3) days from the start of treatment. Friedman tests with Bonferroni correction and Mann–Whitney tests were used (p < .05).ResultsFor the OHDS instrument, it was observed that for the eating and communication domains and an overall score, the treatment with OA positively impacted the lives of T21 patients (p < .05). Regarding the OHIP‐14 instrument, the intragroup evaluation showed that in the CONTROL group, there was no significant difference between the evaluated times; while for the T21 group, there was a significant positive impact (p < .05).ConclusionThe results showed that the treatment with aligners positively impacted the OHRQoL of T21 patients, and these results were perceived by caregivers, mainly in relation to issues related to eating and communication.
Novel mechanism of MicroRNA408 in callus formation from rice mature embryo
Authors: Huang Yizi , Yue Erkui , Lian Guiwei , Lu Jinhan , Ran Le , Ma Shengyun , Wang Kaiqiang , Bai Yu , Han Ning , Bian Hongwu , Guo Fu 
Published: September 2024

SUMMARYMature embryos are the main explants of tissue culture used in rice transgenic technology. However, the mechanism of mature embryo callus formation remains unclear. In this study, a microRNA‐mediated gene regulatory network of rice calli was established using degradome sequencing. We identified a microRNA, OsmiR408, that regulates the formation of the callus derived from the mature rice embryo. OsUCLACYANIN 30 (OsUCL 30), a target gene of OsmiR408, was the most abundant cleavage mRNA in rice callus. OsUCL17 was verified as a target gene of OsmiR408 using RNA ligase‐mediated 5′‐RACE. In analysis of the OsmiR408 promoter reporter line and pri‐miR408 transcript level, the promoter activity and transcript level of MIR408 were increased dramatically during callus formation. In phenotypic observations, OsmiR408 knockout caused severe defects in mature embryo callus formation, whereas OsmiR408 overexpression promoted callus formation. Transcriptome analysis demonstrated that OsUCLs and certain genes related to the plant hormone signal transduction and phenylpropanoid‐flavonoid biosynthesis pathway had different differential expression patterns between OsmiR408 knockout and overexpression calli. Thus, OsmiR408 may regulate callus formation mainly by affecting plant hormone signal transduction and phenylpropanoid‐flavonoid biosynthesis pathway. Our findings provide insight into OsmiR408/UCLs module function in callus formation.
Sodium thiosulfate treatment rescues hyperglycaemia‐induced pronephros damage in zebrafish by upregulating nitric oxide signalling
Authors: Ott Hannes , Bennewitz Katrin , Zhang Xin , Prianichnikova Mariia , Sticht Carsten , Poschet Gernot , Kroll Jens 
Published: September 2024

AbstractSodium thiosulfate (STS) is gaining increasing attention in research for its potential therapeutic applications across a spectrum of disease processes beyond its current uses. However, the precise mechanisms of action remain incompletely understood. We investigated the efficacy of STS in treating hyperglycaemia‐induced pronephros damage in zebrafish to gain further insight into the underlying mechanisms. Hyperglycaemia was induced in zebrafish by suppressing the pdx1 transcription factor, which plays a crucial role in maintaining physiological pancreatic function. STS was administered by introducing it into the medium of zebrafish larvae. The pronephros structure was analysed at 48 h post‐fertilization. Metabolomic profiling and RNA sequencing were conducted on groups exposed to various experimental conditions. Our findings reveal a downregulation of nitric oxide (NO) signalling in zebrafish with a knocked‐down pdx1 gene, both metabolomically and transcriptionally. Notably, treatment with STS led to a compensatory upregulation of the NO signalling, ultimately resulting in the rescue of the pronephros structure. Our study provides compelling evidence that targeting NO metabolism by the administration of STS offers a promising strategy for addressing hyperglycaemia‐induced organ damage. These findings underscore the potential of STS as a promising therapeutic agent for diabetic complications and warrant further investigation of its clinical applications.
imageKey points
Sodium thiosulfate (STS) is increasingly drawing attention in research for its potential therapeutic applications across a spectrum of disease processes.
Here, we demonstrate that STS treatment rescues hyperglycaemia‐induced pronephros damage in zebrafish.
We identified upregulation of nitric oxide signalling as the major driver behind STS‐mediated rescue.
Our data suggest that STS offers a promising strategy for addressing hyperglycaemia‐induced organ damage, including diabetic nephropathy.
Exergy and Entropy Analysis of Heat Exchanger Under Mechanical Vibration and Magnetic Field
Authors: Bhatt Tapasvi , Shah Jainil , Jain Naman , Bhattacharyya Suvanjan 
Published: September 2024

Abstract
The present study primarily investigates the exergy and entropy generation in a heat exchanger influenced by the combined effects of mechanical vibrations and magnetic fields. A rectangular channel with dimensions 40 mm in length and 4 mm in width was examined using magnets of varying strengths and subjected to vibrations and magnetic fields. The Reynolds number (Re) investigated in this study ranges from 150 to 300. Both, magnetic field and vibrations, generated intricate patterns and contours, highlighting their interaction with flow dynamics. As vibrational intensity increased, the Nusselt number amplified correspondingly. While the introduction of magnetic field also enhanced the Nusselt number (Nu), the impact of vibrations was more pronounced. A maximum Nu enhancement of 225.9% was achieved at a Re 300, under the influence of vibrations at 5 mm and 25 Hz, and a magnetic field strength of 2000 G. The study further revealed that exergy efficiency decreases progressively with increasing Re but improves with higher vibrational intensity, reaching a peak of 52.81% at 5 mm and 25 Hz. Additionally, it was observed that irreversibility (φ) decreases with increasing vibrational and magnetic strengths. The ratio of entropy generation under the vibrational and magnetic influence to that of static case peaked at a value of 2.4 under vibrational intensity of 5 mm and 25 Hz, and magnetic field strength of 2000 G.
Inside the Defensive Playbook: Pick-and-Roll Tactical Adjustments Impact the External and Internal Loads During Small-Sided Games in Female Basketball Players
Authors: Qarouach Abdelaziz , Sansone Pierpaolo , Pernigoni Marco , Kreivyte Rasa , Conte Daniele 
Published: September 2024

Purpose: This study aimed to assess the effect of playing different pick-and-roll (PnR) defensive tactical options during small-sided games (SSGs) on external and internal loads in female basketball players. Methods: Twelve female basketball players (age 28 [2] y; stature 175 [6] cm; body mass 65 [7] kg; playing experience 18 [4] y) belonging to a team competing in the Lithuanian second division were recruited for this study. Across 3 experimental sessions and in a randomized order, players performed 3 SSGs sharing the same features but using 3 defensive strategies on the middle PnR action: Switch, Trap, and Drop. External load was measured using PlayerLoad (PL); the number of accelerations, decelerations, and changes of direction, classified based on their intensities as low (<2.5 m·s−2), medium (2.5–3.5 m·s−2), and high (>3.5 m·s−2); and jumps categorized as low (<40 cm) and high (≥40 cm). Internal load was measured via rating of perceived exertion (RPE). Results: Higher PL values in SSGs including Trap defense were found compared with Drop (P < .001, ES = 0.69, moderate) and Switch (P = .001, ES = 0.60, moderate). Additionally, a higher number of accelerations was registered in Trap defense compared with Drop defense (P = .027, ES = 0.99, moderate). Trap defense also led to higher RPE compared with Switch (P = .003, ES = 1.49, large) and Drop (P = .004, ES = 1.42, large) defense. Conclusions: Different defensive strategies on the middle PnR can influence the external and internal loads during SSGs, and female basketball coaches should consider the high demand of the Trap defense when designing SSGs.
Perceptions of Parents of Children With Disabilities Toward Physical Education: A Systematic Review
Authors: Forbes Adam S. , Arroyo-Rojas Fabián , Block Martin E. 
Published: September 2024

Physical education (PE) has a unique opportunity in not only supporting children with disabilities but also their parents’ physical-activity knowledge and support behaviors. Therefore, the purpose of this systematic literature review was to synthesize published studies regarding parent perspectives toward physical education (PE) for their children with disabilities. A total of 19 articles met inclusion criteria. Three themes emerged: (a) parents’ understanding of adapted PE (A/PE), (b) parents’ expectations of A/PE teachers, and (c) parents’ undeveloped relationships with A/PE teachers. There exists a disconnection between parent expectations and PE teachers’ abilities to accommodate their children and develop lines of communication. Additionally, parental value toward PE was often lower compared with other areas of need for their children. Future research suggests exploring teacher perspectives in understanding the relationships with parents. Furthermore, exploring the origins of parental values for PE and its impact on their perspectives warrants further investigation.
An adaptable, fit-for-purpose screening approach with high-throughput capability to determine speed of action and stage specificity of anti-malarial compounds
Authors: Duffy Sandra , Sleebs Brad E. , Avery Vicky M. 
Published: September 2024

ABSTRACT

A revamped
in vitro
compound identification and activity profiling approach is required to meet the large unmet need for new anti-malarial drugs to combat parasite drug resistance. Although compound hit identification utilizing high-throughput screening of large compound libraries is well established, the ability to rapidly prioritize such large numbers for further development is limited. Determining the speed of action of anti-malarial drug candidates is a vital component of malaria drug discovery, which currently occurs predominantly in lead optimization and development. This is due in part to the capacity of current methods which have low throughput due to the complexity and labor intensity of the approaches. Here, we provide an adaptable screening paradigm utilizing automated high content imaging, including the development of an automated schizont maturation assay, which collectively can identify anti-malarial compounds, classify activity into fast and slow acting, and provide an indication of the parasite stage specificity, with high-throughput capability. By frontloading these critical biological parameters much earlier in the drug discovery pipeline, it has the potential to reduce lead compound attrition rates later in the development process. The capability of the approach in its alternative formats is demonstrated using three Medicines for Malaria Venture open access compound “boxes,” namely Pathogen Box (malaria set—125 compounds), Global Health Priority Box [Malaria Box 2 (80 compounds) and zoonotic neglected diseases (80 compounds)], and the Pandemic Response Box (400 compounds). From a total of 685 compounds tested, 79 were identified as having fast ring-stage-specific activity comparable to that of artemisinin and therefore of high priority for further consideration and development.
Complete genomes of two cluster AK
Arthrobacter
phages isolated from soil samples in Newburgh, NY, United States
Authors: Delesalle Véronique A. , King Mariah A.K. , Rozario Tabitha J. , Wolf Noah D. , Stewart Connor J. , DeMato Luke F. , Trafford Kevin F. , Adhikari Saiman , Dinh Van T. , Caputo Gina , Hunter Ashley , Licata Michelle , Modell Misun , Bhalla Suparna 
Published: September 2024

ABSTRACT

Two phages belonging to
Arthrobacter
phage cluster AK were isolated from soil samples collected in Newburgh, NY in 2021. Both are lytic with a genome organization typical of siphoviruses except for two genes encoding minor tail proteins with pyocin-knob domains found early in the genome, before the terminase gene.
Do infographics ‘spin’ the findings of health and medical research?
Authors: Muller Ryan , Ferreira Giovanni , Bejarano Geronimo , Gamble Andrew R , Kirk James , Sindone James , Zadro Joshua R 
Published: September 2024

ObjectiveTo compare the prevalence of ‘spin’, and specific reporting strategies for spin, between infographics, abstracts and full texts of randomised controlled trials (RCTs) reporting non-significant findings in the field of health and medicine and to assess factors associated with the presence of spin.DesignCross-sectional observational study.Data sourcePublications in top quintile health and medical journals from August 2018 to October 2020 (Journal Citation Reports database).Eligibility criteriaInfographics, abstracts and full texts of RCTs with non-significant results for a primary outcome.Main outcome(s) and measure(s)Presence of spin (any spin and spin in the results and conclusions of infographics, abstracts and full texts).Exposure(s)Conflicts of interest, industry sponsorship, trial registration, journal impact factor, spin in the abstract, spin in the full text.Results119 studies from 40 journals were included. One-third (33%) of infographics contained spin. Infographics were not more likely to contain any spin than abstracts (33% vs 26%, OR 1.4; 95% CI 0.8 to 2.4) or full texts (33% vs 26%, OR 1.4; 95% CI 0.8 to 2.4). Higher journal impact factor was associated with slightly lower odds of spin in infographics and full texts, but not abstracts. Infographics, but not abstracts or full texts, were less likely to contain spin if the trial was prospectively registered. No other significant associations were found.ConclusionsNearly one-third of infographics spin the findings of RCTs with non-significant results for a primary outcome, but the prevalence of spin is not higher than in abstracts and full texts. Given the increasing popularity of infographics to disseminate research findings, there is an urgent need to improve the reporting of research in infographics.
Serum amyloid A for predicting prognosis in patients with newly diagnosed Crohn’s disease
Authors: Chen Qia , Zhang Xi , Tie Yizhe , Zhang Jianwu , Huang Pinwei , Xie Yuxuan , Zhang Liqian , Tang Xueer , Zeng Zhirong , Li Li , Chen Minhu , Chen Rirong , Zhang Shenghong 
Published: September 2024

ObjectiveSerum amyloid A (SAA) was found to be positively correlated with the activity of Crohn’s disease (CD); however, its prognostic value remains uncertain. Here, we examined its predictive ability in newly diagnosed CD and explored genetic association.MethodsThis retrospective cohort study included patients newly diagnosed as CD at the First Affiliated Hospital of Sun Yat-sen University between June 2010 and March 2022. We employed receiver operating characteristic curve, Cox proportional hazard regression models and restricted cubic splines to investigate the prognostic performance of SAA for surgery and disease progression. To assess possible causality, a two-sample Mendelian randomisation (MR) of published genome-wide association study data was conducted.ResultsDuring 2187.6 person-years (median age, 28 years, 72.4% male), 87 surgery and 153 disease progression events were documented. A 100-unit increment in SAA level generated 14% higher risk for surgery (adjusted HR (95% CI): 1.14 (1.05–1.23), p=0.001) and 12% for disease progression (1.12 (1.05–1.19), p<0.001). Baseline SAA level ≥89.2 mg/L led to significantly elevated risks for surgery (2.08 (1.31–3.28), p=0.002) and disease progression (1.72 (1.22–2.41), p=0.002). Such associations were assessed as linear. Adding SAA into a scheduled model significantly improved its predictive performances for surgery and disease progression (p for net reclassification indexes and integrated discrimination indexes <0.001). Unfortunately, no genetic causality between SAA and CD was observed in MR analysis. Sensitivity analyses showed robust results.ConclusionAlthough causality was not found, baseline SAA level was an independent predictor of surgery and disease progression in newly diagnosed CD, and had additive benefit to existing prediction models.
What types of objective measures have been used to assess core ADHD symptoms in children and young people in naturalistic settings? A scoping review
Authors: Kelman Charlotte Rose , Thompson Coon Jo , Ukoumunne Obioha C , Moore Darren , Gudka Rebecca , Bryant Eleanor F , Russell Abigail 
Published: September 2024

ObjectivesWe described the range and types of objective measures of attention-deficit/hyperactivity disorder (ADHD) in children and young people (CYP) reported in research that can be applied in naturalistic settings.DesignScoping review using best practice methods.Data SourcesMEDLINE, APA PsycINFO, Embase, (via OVID); British Education Index, Education Resources Information Centre, Education Abstracts, Education Research Complete, Child Development and Adolescent Papers, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Psychology and Behavioural Sciences Collection (via EBSCO) were searched between 1 December 2021 and 28 February 2022.Eligibility CriteriaPapers reported an objective measure of ADHD traits in CYP in naturalistic settings written in English.Data extraction and synthesis2802 papers were identified; titles and abstracts were screened by two reviewers. 454 full-text papers were obtained and screened. 128 papers were eligible and included in the review. Data were extracted by the lead author, with 10% checked by a second team member. Descriptive statistics and narrative synthesis were used.ResultsOf the 128 papers, 112 were primary studies and 16 were reviews. 87% were conducted in the USA, and only 0.8% originated from the Global South, with China as the sole representative. 83 objective measures were identified (64 observational and 19 acceleration-sensitive measures). Notably, the Behaviour Observation System for Schools (BOSS), a behavioural observation, emerged as one of the predominant measures. 59% of papers reported on aspects of the reliability of the measure (n=76). The highest inter-rater reliability was found in an unnamed measure (% agreement=1), Scope Classroom Observation Checklist (% agreement=0.989) and BOSS (% agreement=0.985). 11 papers reported on aspects of validity. 12.5% of papers reported on their method of data collection (eg, pen and paper, on an iPad). Of the 47 papers that reported observer training, 5 reported the length of time the training took ranging from 3 hours to 1 year. Despite recommendations to integrate objective measures alongside conventional assessments, use remains limited, potentially due to inconsistent psychometric properties across studies.ConclusionsMany objective measures of ADHD have been developed and described, with the majority of these being direct behavioural observations. There is a lack of reporting of psychometric properties and guidance for researchers administering these measures in practice and in future studies. Methodological transparency is needed. Encouragingly, recent papers begin to address these issues.
Acceptability and timing considerations when administering patient-reported outcome measures (PROMs) among people with chronic health conditions who are culturally and linguistically diverse (CALD): a qualitative study protocol
Authors: Nikolovski Jessica , Morton Rachael L. , Mercieca-Bebber Rebecca , Armstrong Matilda , Hartas Gill , Rossiter Brad , Fagan Margaret , Tinsley Melissa , Snyder Claire , Aiyegbusi Olalekan Lee , Amin-Korim Rubina , Sutherland Kim , Rutherford Claudia 
Published: September 2024

IntroductionPatient-reported outcome measures (PROMs) are validated and standardised questionnaires that capture patients’ own reports of their symptoms, functioning and well-being. PROMs can facilitate communication between patients and clinicians, reduce symptom burden, enhance quality of life and inform health service re-design. We aim to determine the acceptability of PROMs and the preferred timing of PROM completion in New South Wales (NSW) at the point of care, facilitated by the Health Outcomes and Patient Experiences (HOPE) platform.Methods and analysisSemi-structured interviews with patients (~50-75, sampling across seven language groups and seven clinical cohorts), carers (~10–20) and clinicians (~18) enrolled in HOPE will be conducted via videoconference, telephone or in person. Participants will be asked questions about (1) what makes PROMs acceptable for use in chronic disease management (2) when patients would prefer to complete PROMs and when clinicians would like to use PROMs for clinical decision-making and (3) factors that impede the acceptability of PROMs for culturally and linguistically diverse patients. Interviews will be analysed using a reflexive thematic approach, guided by Normalisation Process Theory.Ethics and disseminationEthics approval has been obtained from the Sydney Local Health District Human Research Ethics Committee (SLHD HREC, Study Protocol #X24-0138). Results will be published in appropriate peer-reviewed journals, presented at conferences, disseminated to participants in the form of a plain language summary, and widely disseminated to consumer groups and professional stakeholders.