bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of MD1 exacerbates pressure overload-induced left ventricular structural and electrical remodelling
Authors: Peng Jianye , Liu Yu , Xiong Xiaoju , Huang Congxin , Mei Yang , Wang Zhiqiang , Tang Yanhong , Ye Jing , Kong Bin , Liu Wanli , Wang Teng , Huang He 
Published: July 2017

AbstractMyeloid differentiation protein 1 (MD1) has been implicated in numerous pathophysiological processes, including immune regulation, obesity, insulin resistance, and inflammation. However, the role of MD1 in cardiac remodelling remains incompletely understood. We used MD1-knockout (KO) mice and their wild-type littermates to determine the functional significance of MD1 in the regulation of aortic banding (AB)-induced left ventricular (LV) structural and electrical remodelling and its underlying mechanisms. After 4 weeks of AB, MD1-KO hearts showed substantial aggravation of LV hypertrophy, fibrosis, LV dilation and dysfunction, and electrical remodelling, which resulted in overt heart failure and increased electrophysiological instability. Moreover, MD1-KO-AB cardiomyocytes showed increased diastolic sarcoplasmic reticulum (SR) Ca2+leak, reduced Ca2+transient amplitude and SR Ca2+content, decreased SR Ca2+-ATPase2 expression, and increased phospholamban and Na+/Ca2+-exchanger 1 protein expression. Mechanistically, the adverse effects of MD1 deletion on LV remodelling were related to hyperactivated CaMKII signalling and increased impairment of intracellular Ca2+homeostasis, whereas the increased electrophysiological instability was partly attributed to exaggerated prolongation of cardiac repolarisation, decreased action potential duration alternans threshold, and increased diastolic SR Ca2+leak. Therefore, our study on MD1 could provide new therapeutic strategies for preventing/treating heart failure.
Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
Authors: Baba Kenkichi , DeBruyne Jason P. , Tosini Gianluca 
Published: July 2017

AbstractMany of the physiological, cellular, and molecular rhythms that are present within the eye are under the control of circadian clocks. Experimental evidence suggests that the retinal circadian clock, or its output signals (e.g., dopamine and melatonin), may contribute to eye disease and pathology. We recently developed a retinal pigment ephithelium (RPE)-choroid preparation to monitor the circadian clock using PERIOD2 (PER2)::LUC knock-in mouse. In this study we report that dopamine, but not melatonin, is responsible for entrainment of the PER2::LUC bioluminescence rhythm in mouse RPE-choroid. Dopamine induced phase-advances of the PER2::LUC bioluminescence rhythm during the subjective day and phase-delays in the late subjective night. We found that dopamine acts exclusively through Dopamine 2 Receptors to entrain the circadian rhythm in PER2::LUC bioluminescence. Finallly, we found that DA-induced expression of core circadian clock genes Period1 and Period2 accompanied both phase advances and phase delays of the RPE-choroid clock, thus suggesting that – as in other tissues – the rapid induction of these circadian clock genes drives the resetting process. Since the RPE cells persist for the entire lifespan of an organism, we believe that RPE-choroid preparation may represent a new and unique tool to study the effects of circadian disruption during aging.
Uniaxial orientation of P3HT film prepared by soft friction transfer method
Authors: Imanishi Masayoshi , Kajiya Daisuke , Koganezawa Tomoyuki , Saitow Ken-ichi 
Published: July 2017

AbstractThe realization of room-temperature processes is an important factor in the development of flexible electronic devices composed of organic materials. In addition, a simple and cost-effective process is essential to produce stable working devices and to enhance the performance of a smart material for flexible, wearable, or stretchable-skin devices. Here, we present a soft friction transfer method for producing aligned polymer films; a glass substrate was mechanically brushed with a velvet fabric and poly(3-hexylthiophene) (P3HT) solution was then spin-coated on the substrate. A P3HT film with a uniaxial orientation was obtained in air at room temperature. The orientation factor was 17 times higher than that of a film prepared using a conventional friction transfer technique at a high temperature of 120 °C. In addition, an oriented film with a thickness of 40 nm was easily picked up and transferred to another substrate. The mechanism for orientation of the film was investigated using six experimental methods and theoretical calculation, and was thereby attributed to a chemical process, i.e., cellulose molecules attach to the substrate and act as a template for molecular alignment.
English Verb Accuracy of Bilingual Cantonese–English Preschoolers
Authors: Rezzonico Stefano , Goldberg Ahuva , Milburn Trelani , Belletti Adriana , Girolametto Luigi 
Published: July 2017

PurposeKnowledge of verb development in typically developing bilingual preschoolers may inform clinicians about verb accuracy rates during the 1st 2 years of English instruction. This study aimed to investigate tensed verb accuracy in 2 assessment contexts in 4- and 5-year-old Cantonese–English bilingual preschoolers.MethodThe sample included 47 Cantonese–English bilinguals enrolled in English preschools. Half of the children were in their 1st 4 months of English language exposure, and half had completed 1 year and 4 months of exposure to English. Data were obtained from the Test of Early Grammatical Impairment (Rice & Wexler, 2001) and from a narrative generated in English.ResultsBy the 2nd year of formal exposure to English, children in the present study approximated 33% accuracy of tensed verbs in a formal testing context versus 61% in a narrative context. The use of the English verb BE approximated mastery. Predictors of English third-person singular verb accuracy were task, grade, English expressive vocabulary, and lemma frequency.ConclusionsVerb tense accuracy was low across both groups, but a precocious mastery of BE was observed. The results of the present study suggest that speech-language pathologists may consider, in addition to an elicitation task, evaluating the use of verbs during narratives in bilingual Cantonese–English bilingual children.
Secondary Reconstruction of Posttraumatic Enophthalmos with Titanium Mesh and Buccal Fat Pad Graft: Case Report
Authors: Santos Roberto , Cupello Viviane , Polinati José Emilio , Paredes Leonardo , Gaffrée Gustavo 
Published: July 2017

AbstractSecondary enophthalmos caused by an untreated orbital blowout fracture can cause esthetic and functional disturbances. The esthetic defect is manifested by sinking of the superior sulcus and the hypophthalmic globe. Functional impairment of the eye can usually be a common complaint with restriction of eye motion and diplopia. Early diagnosis followed by repair of surgically correctable fractures is the most acceptable procedure. Failure in the primary treatment may cause scar contraction and fat atrophy. The aim of this paper is to report a case of a late treatment of blowout orbital floor fracture with secondary enophthalmos using titanium mesh and buccal fat pad graft.
Toxicity of Liver-Directed Therapies for Hepatocellular Carcinoma
Authors: Lee Andrew , Fidelman Nicholas 
Published: July 2017

AbstractThe purpose of this review is to outline the various toxicities of liver-directed therapy approaches employed for treatment of liver-limited hepatocellular carcinoma. The covered topics span toxicities related to ablative and transarterial treatment approaches, including radiofrequency and microwave ablation, cryoablation, percutaneous ethanol injection, transarterial chemoembolization, and yttrium-90 radioembolization. Both common entities, such as postablation, and post(radio)embolization syndrome and hepatotoxicity, as well as uncommon topics (nontarget organ damage, cryoshock, infections, bile duct injuries) are described. Appropriate treatments for management of these side effects are suggested.
Psychopathologische Befunderhebung bei der homöopathischen Erstanamnese
Authors: Besserer Kai 
Published: July 2017

ZusammenfassungBei vielen Beschwerde- und Krankheitsbildern, mit denen Patienten eine homöopathische Behandlung aufsuchen, spielen psychische Befindlichkeiten, Symptome oder manifeste psychiatrische Erkrankungen eine wichtige, wenn nicht zentrale Rolle. Die psychopathologische Befunderhebung sollte daher Bestandteil einer ausführlichen homöopathischen Anamnese sein. Im Folgenden werden der Inhalt und die Durchführung einer psychopathologischen Befunderhebung dargestellt und erläutert. Entsprechende Repertoriumsrubriken werden exemplarisch vorgeschlagen.
Frühe Nutzenbewertung neu zugelassener Arzneimittel – Methodische Anforderungen an die Daten zur
Lebensqualität
Authors: Thiem Helena 
Published: July 2017

Blome C et al. Four years of early benefit assessment of new drugs in Germany: a qualitative study on methodological requirements for quality
of life data. Eur J Health Econ 2017; 18: 181–193
Der Gemeinsame Bundesausschuss (G-BA) führt für alle neu zugelassenen Arzneimittel eine frühe (Zusatz-)Nutzenbewertung durch. In die
Entscheidung mit einbezogen wird die Auswirkung auf unterschiedliche Endpunkte, u. a. auch auf die Lebensqualität (QoL). Die Autoren
haben für die zwischen den Jahren 2011–2014 veröffentlichten Nutzenbewertungen analysiert, inwieweit die Daten zur Lebensqualität bei
der abschließenden Bewertung zum Tragen kommen.
Management in der Gefäßmedizin – Therapie mit PCSK9-Inhibitoren derzeit unwirtschaftlich für
US-Gesundheitssystem
Authors: Dobler Gabriele 
Published: July 2017

Kazi DS et al. Cost-effectiveness of PCSK9 Inhibitor Therapy in Patients With Heterozygous Familial Hypercholesterolemia or Atherosclerotic
Cardiovascular Disease. JAMA 2016; 316: 743–753
PCSK9-Inhibitoren wurden kürzlich in den USA für Patienten mit familiärer Hypercholesterinämie (FH) oder atherosklerotischer
kardiovaskulärer Erkrankung (ASCVD) zugelassen, die trotz Maximaldosen von Statinen eine weitere Senkung des LDL-Cholesterins benötigen.
Langfristig könnten sie wichtig bei der ASCVD-Prophylaxe werden. Die Autoren untersuchten die langfristige Wirtschaftlichkeit und
potentiellen Kosten für das US-Gesundheitssystem.
Impaired Metabolism of Selenomethionine in Graves’ Disease: A Biokinetics Study of Soft Gel Capsule Formulation
Authors: Duntas Leonidas , Boutsiadis Anastasios , Tsakris Athanasios 
Published: July 2017

AbstractPatients with Graves’ disease are known to have low selenium (Se) status, Se supplementation resulting in clinical and biochemical improvement. Selenomethionine (SeMet) in a new soft gel capsule formulation was used in a pilot study in 6 patients with acute Graves’ disease and low selenium levels (61.3±12.9 μg/l) before and in 4/6 patients 3 months after combined treatment with methimazole and SeMet 200 μg/day (113.3±46.3 μg/l), as well as in 6 euthyroid controls (82±11.8 μg/l). The biokinetics were studied following ingestion of 200 μg SeMet (single dose) soft gel capsule, Se serum concentrations being measured at various time points within 24 h. Se levels rose variably in all patients and controls. While levels peaked in all subjects following 8 h of intake, the increase was somewhat slower in acute hyperthyroidism as compared to 3 months later when these patients had been rendered euthyroid, this possibly due to derangement of Se storage capacity by SEPP or increased requirements in the acute phase of the disease, leading to depletion of the trace element. The compound was shown to be bioavailable and safe and patients treated for 3 months exhibited higher Se levels at the different time points. These findings are of major importance for sufferers of GD since they indicate that early Se supplementation, with its beneficial antioxidant impact on inflammatory activity, could slow, or possibly even forestall, the clinical progression of the disease.
Does Bioelectrical Impedance Analysis Accurately Estimate the Physiological Condition of Threatened and Endangered Desert Fish Species?
Authors: Dibble Kimberly L. , Yard Michael D. , Ward David L. , Yackulic Charles B. 
Published: July 2017

AbstractBioelectrical impedance analysis (BIA) is a nonlethal tool with which to estimate the physiological condition of animals that has potential value in research on endangered species. However, the effectiveness of BIA varies by species, the methodology continues to be refined, and incidental mortality rates are unknown. Under laboratory conditions we tested the value of using BIA in addition to morphological measurements such as total length and wet mass to estimate proximate composition (lipid, protein, ash, water, dry mass, energy density) in the endangered Humpback Chub Gila cypha and Bonytail G. elegans and the species of concern Roundtail Chub G. robusta and conducted separate trials to estimate the mortality rates of these sensitive species. Although Humpback and Roundtail Chub exhibited no or low mortality in response to taking BIA measurements versus handling for length and wet‐mass measurements, Bonytails exhibited 14% and 47% mortality in the BIA and handling experiments, respectively, indicating that survival following stress is species specific. Derived BIA measurements were included in the best models for most proximate components; however, the added value of BIA as a predictor was marginal except in the absence of accurate wet‐mass data. Bioelectrical impedance analysis improved the R2 of the best percentage‐based models by no more than 4% relative to models based on morphology. Simulated field conditions indicated that BIA models became increasingly better than morphometric models at estimating proximate composition as the observation error around wet‐mass measurements increased. However, since the overall proportion of variance explained by percentage‐based models was low and BIA was mostly a redundant predictor, we caution against the use of BIA in field applications for these sensitive fish species.Received November 7, 2016; accepted March 1, 2017 Published online July 5, 2017
Renal Cystic Adenocarcinoma in a Flowerhorn Cichlid with Metastatic Involvement of the Spleen
Authors: Rahmati-Holasoo Hooman , Shokrpoor Sara , Masoudifard Majid , Ebrahimzadeh Mousavi HosseinAli , Haddadi Ali , Tavakkoli Amir 
Published: July 2017

Abstract
A 480-g flowerhorn cichlid (an ornamental hybrid) with severe bilateral abdominal swelling, bulla-like structures on the skin, bilateral exophthalmia, and a prolapsed intestine was presented. Radiographs showed compression of the posterior part of the swim bladder and abdominal distention. Ultrasonography of visceral organs revealed a heterogeneous mass with hypoechoic to anechoic polycystic parenchyma and free fluid in the abdominal cavity. At necropsy, free fluid in the abdominal cavity and a large polycystic mass originating from the posterior kidney were observed. Histologically, the mass was composed of more cystic growth of tubules. The renal architecture was replaced by tubules, often irregular in shape, lined by simple to lightly stratified layers of neoplastic and pleomorphic cuboidal to columnar epithelial cells and the absence of glomeruli. Birefringent crystals were observed with polarized light within the lumen of some tubules. The apical border of the neoplastic cells was periodic acid–Schiff positive. Immunohistochemically, the neoplastic cells were positive for cytokeratin AE1/AE3 and proliferating cell nuclear antigen and were negative for p53 (tumor suppressor protein). Microscopic metastasis was seen in the spleen. The metastatic tumor was classified as a cystic adenocarcinoma of the kidney, originating from the proximal tubules.
Received October 7, 2016; accepted June 18, 2017 Published online July 31, 2017
Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis
Authors: Hidese Ryota , Im Ki-Hwan , Kobayashi Masaki , Niitsu Masaru , Furuchi Takemitsu , Fujiwara Shinsuke 
Published: July 2017

Abstract
Long/branched-chain polyamines are unique polycations found in thermophiles. The hyperthermophilic archaeon Thermococcus kodakarensis contains spermidine and a branched-chain polyamine, N4-bis(aminopropyl)spermidine, as major polyamines. The metabolic pathways associated with branched-chain polyamines remain unknown. Here, we used gas chromatography and liquid chromatography-tandem mass spectrometry analyses to identify a new acetylated polyamine, N4-bis(aminopropyl)-N1-acetylspermidine, from T. kodakarensis; this polyamine was not found in other micro-organisms. The amounts of branched-chain polyamine and its acetylated form increased with temperature, indicating that branched-chain polyamines are important for growth at higher temperatures. The amount of quaternary acetylated polyamine produced was associated with the amount of N4-bis(aminopropyl)spermidine in the cell. The ratio of acetylated to non-acetylated forms was higher in the stationary phase than in the logarithmic growth phase under high-temperature stress condition.
Habitat Selection Important for Red Snapper Feeding Ecology in the Northwestern Gulf of Mexico
Authors: Schwartzkopf Brittany D. , Langland Todd A. , Cowan James H. 
Published: July 2017

Abstract
The present study compared Red Snapper Lutjanus campechanus feeding ecology among seasons, sizes, and natural reefs, and between natural and artificial reefs located on the Louisiana shelf edge. As low-quality diets may hinder growth, reduce condition, and lead to poor reproductive output, information on diets for various habitat types occupied by Red Snapper is critical. This study utilized both stomach content analysis and nitrogen (δ15N) and carbon (δ13C) stable isotope analysis of muscle tissue. Fishes dominated Red Snapper diets at all sites, in all seasons, and generally for all size-classes. A high biomass of zooplankton was consumed at the artificial reef area and during spring. The largest natural reef provided the widest diversity of prey items, and there was little overlap in prey species consumed between natural and artificial reef habitats. Higher δ15N and lower δ13C values were found for Red Snapper at the artificial reef area, and contrary to the diet results, distinct isotopic niches were found between natural reefs. Differences in Red Snapper isotopic niches between natural reefs indicate that individuals may remain in residence on a specific reef for an extended period. Red Snapper at natural reefs were found to feed on and above the reef, whereas feeding at artificial reefs predominantly occurred on the surrounding seafloor and up in the water column. The quality of habitats underlying reefs is important, as Red Snapper diets reflected the substrate at the occupied reefs. In the northwestern Gulf of Mexico, large, complex natural reefs may provide higher habitat quality for Red Snapper with respect to prey resources than artificial reefs located on mud substrates. Determining which habitats provide high-quality prey resources for Red Snapper in the Gulf of Mexico may assist decisions on policies and regulations for habitat protection as well as decisions on the future placement of artificial reefs.
Banking on Living Kidney Donors—A New Way to Facilitate Donation without Compromising on Ethical Values
Authors: Martin Dominique E , Danovitch Gabriel M 
Published: July 2017

AbstractPublic surveys conducted in many countries report widespread willingness of individuals to donate a kidney while alive to a family member or close friend, yet thousands suffer and many die each year while waiting for a kidney transplant. Advocates of financial incentive programs or “regulated markets” in kidneys present the problem of the kidney shortage as one of insufficient public motivation to donate, arguing that incentives will increase the number of donors. Others believe the solutions lie—at least in part—in facilitating so-called “altruistic donation;” harnessing the willingness of relatives and friends to donate by addressing the many barriers which serve as disincentives to living donation. Strategies designed to minimize financial barriers to donation and the use of paired kidney exchange programs are increasingly enabling donation, and now, an innovative program designed to address what has been termed “chronologically incompatible donation” is being piloted at the University of California, Los Angeles, and elsewhere in the United States. In this program, a person whose kidney is not currently required for transplantation in a specific recipient may instead donate to the paired exchange program; in return, a commitment is made to the specified recipient that priority access for a living-donor transplant in a paired exchange program will be offered when or if the need arises in the future. We address here potential ethical concerns related to this form of organ “banking” from living donors, and argue that it offers significant benefits without undermining the well-established ethical principles and values currently underpinning living donation programs.
Biomechanical control of lysosomal secretion via the VAMP7 hub: a tug-of-war mechanism between VARP and LRRK1
Authors: Wang Guan , Nola Sébastien , Bovio Simone , Coppey-Moisan Maïté , Lafont Frank , Galli Thierry 
Published: July 2017

AbstractThe rigidity of the cell environment can vary tremendously between tissues and in pathological conditions. How this property may affect intracellular membrane dynamics is still largely unknown. Here, using atomic force microscopy, we found that cells deficient in the secretory lysosome v-SNARE VAMP7 were impaired in adapting to substrate rigidity. Conversely VAMP7-mediated secretion was stimulated by more rigid substrate and this regulation depended on the Longin domain of VAMP7. We further found that the Longin domain bound the kinase and retrograde trafficking adaptor LRRK1 and LRRK1 negatively regulated VAMP7-mediated exocytosis. Conversely, VARP, a VAMP7- and kinesin 1-interacting protein, further controlled the availability for secretion of peripheral VAMP7 vesicles and response of cells to mechanical constraints. We propose a mechanism whereby biomechanical constraints regulate VAMP7- dependent lysosomal secretion via LRRK1 and VARP tug-of-war control of the peripheral readily- releasable pool of secretory lysosomes.
Inevitable knowledge strategy
Authors: P. Arunprasad 
Published: July 2017

PurposeThe purpose of this paper is to determine the impact of strategic human resource management (HRM) practices and knowledge strategies on the performance of a sample of software companies in India.Design/methodology/approachThe sample chosen for this study was software professionals in India; the software companies were chosen based on their listing in the NASSCOM annual report, with financial turnover as the basis for classification. In order to measure the tangible and intangible outcomes, instruments used in this study include financial and non-economic performance.FindingsAs per the contingency approach, the fit between strategic HRM practices (staffing, general training, specific training, performance appraisal, performance feedback, reward and compensation and employee development) and knowledge strategy dimensions (consolidator, transformer and co-inventor) was observed and the results revealed that the moderation effect has had a positive impact on the firms’ performance.Practical implicationsInvestment in specific developmental programs for high-potential employees and quick learners will make knowledge-intensive firms financially sound in the long run. Through an appropriate reward strategy, employees who are capable of lateral and innovative thinking at work can be engaged in consistently delivering quality projects, which will have a significant positive impact on overall project costs and the financial performance of the firm.Originality/valueThe proposed model in this study can enhance a firm’s performance, provided the firm adopts a specific knowledge strategy and coherently aligns it with strategic HRM practices to achieve a sustained competitive advantage.