bluuebunny/crossref_metadata_2025_split huggingface dataset:
TCDD induces UbcH7 expression and synphilin‐1 protein degradation in the mouse ventral midbrain
Authors: González‐Barbosa Emmanuel , Mejía‐García Alejandro , Bautista Elizabeth , Gonzalez Frank J. , Segovia José , Elizondo Guillermo 
Published: June 2017

AbstractUbcH7 is an ubiquitin‐conjugating enzyme that interacts with parkin, an E3 ligase. The UbcH7–parkin complex promotes the ubiquitination and degradation of several proteins via the 26S proteasome. Cellular accumulation of the UbcH7–parkin targets alpha‐synuclein and synphilin‐1 has been associated with Parkinson disease. In mouse liver, 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin, an aryl hydrocarbon receptor ligand, induces UbcH7 expression. Therefore, the aim of the present study was to determine whether 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin induces Ubch7 mRNA and UbcH7 protein expression in the mouse brain, to characterize the molecular mechanism, and the effect on synphilin‐1 half‐life. We found that 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin promotes the aryl hydrocarbon receptor binding to Ubch7 gene promoter as well as its transactivation, resulting in an induction of UbcH7 levels in the olfactory bulb, ventral midbrain, hippocampus, striatum, cerebral cortex, brain stem, and medulla oblongata. In parallel, 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin promoted synphilin‐1 degradation in an aryl hydrocarbon receptor‐dependent way.
Development of a Cell‐Based Gene Therapy Approach to Selectively Turn Off Bone Formation
Authors: Alvarez‐Urena Pedro , Zhu Banghe , Henslee Gabrielle , Sonnet Corinne , Davis Eleanor , Sevick‐Muraca Eva , Davis Alan , Olmsted‐Davis Elizabeth 
Published: June 2017

ABSTRACTCell and gene therapy approaches are safer when they possess a system that enables the therapy to be rapidly halted. Human mesenchymal stem cells were transduced with an adenoviral vector containing the cDNA for bone morphogenetic protein 2 (AdBMP2) to induce bone formation. To make this method safer, a system to quickly kill these virally transduced cells was designed and evaluated. Cells were encapsulated inside poly(ethylene glycol) diacrylate (PEG‐Da) hydrogels that are able to shield the cells from immunological destruction. The system involves an inducible caspase‐9 (iCasp9) activated using a specific chemical inducer of dimerization (CID). Delivering AdBMP2‐transduced human mesenchymal stem cells encapsulated in PEG‐Da hydrogel promoted ectopic ossification in vivo, and the iCasp9 system allowed direct control of the timing of apoptosis of the injected cells. The iCasp9‐CID system enhances the safety of delivering AdBMP2‐transduced cells, making it a more compelling therapeutic for bone repair and spine fusion. J. Cell. Biochem. 118: 3627–3634, 2017. © 2017 Wiley Periodicals, Inc.
Study on morphological characteristics and microscopic structure of medicinal organs of Pulsatilla chinensis (Bunge) Regel
Authors: Wang Wen‐Hao , Yang Jun , Peng Hua‐Sheng , Qian Jiang‐Ping 
Published: June 2017

AbstractModern clinical studies have found that Pulsatilla radix contains a variety of active ingredients; however, its medicinal parts and microstructure have been controversial. Based on morphological observation and microscopic identification of different structures, we chose Anhui, Henan, Jilin, and Shanxi Province to study Pulsatilla chinensis (Bunge) Regel populations. We specifically addressed different diameters and anatomical structures of different parts of underground organs. We also found that P. chinensis (Bunge) Regel had a “joint point” structure in its underground organ. Above the “joint point” is the rhizome, below the “joint point” is the root. The main medicinal organ is the rhizome. The protective tissues of the endothelial layer of P. chinensis (Bunge) Regel changed in the process of its development. The protective tissues were replaced by epidermis, cortex, endodermis, and phloem. With the secondary growth, the endothelial cells have been radial division. This study showed that morphology and microscopic identification was an important mean of medicinal material identifications, and it had the characteristics of a convenient, fast, and intuitive method to identify the composition and structural characteristics of P. chinensis (Bunge) Regel medicinal parts.
A Facial Synthesis and Anticancer Activity of (Z)‐2‐((5‐(4‐nitrobenzylidene)‐4‐oxo‐4,5‐dihydrothiazol‐2‐yl)amino)‐substituted Acid
Authors: Pansare Dattatraya N. , Shelke Rohini N. , Shinde Devanand B. 
Published: June 2017

In order to explore the anticancer and antimicrobial activity associated with the thiazole framework, we synthesized the new series (Z)‐2‐((5‐(4‐nitrobenzylidene)‐4‐oxo‐4,5‐dihydrothiazol‐2‐yl)amino)‐substituted acid derivatives 6a–l. All the synthesized compounds were evaluated for anticancer and antimicrobial activity in vitro. Among these, the compounds 6a, 6b, 6c, 6e, 6f, 6g, 6h, 6i, 6j, and 6k showed highest antibacterial and antifungal activity. The compound 6a exhibited significant antibacterial activity against Bacillussubtilis, whereas compound 6j displays significant antifungal activity against fungal strains, that is, A. oryzae. The in vitro anticancer studies revealed that 6e, 6g, 6h, 6k, and 6l are the most active compounds against MCF‐7 and BT‐474 human breast cancer cell lines, which can be regarded as the promising drug candidate for development of anticancer drugs.
An application of CIFAP for predicting the binding affinity of Chk1 inhibitors derived from 2‐aminothiazole‐4‐carboxamide
Authors: Konyar Dilan , Erdas Ozlem , Alpaslan Ferda Nur , Buyukbingol Erdem 
Published: June 2017

AbstractInvestigation of protein‐ligand interactions obtained from experiments has a crucial part in the design of newly discovered and effective drugs. Analyzing the data extracted from known interactions could help scientists to predict the binding affinities of promising ligands before conducting experiments. The objective of this study is to advance the CIFAP (compressed images for affinity prediction) method, which is relevant to a protein‐ligand model, identifying 2D electrostatic potential images by separating the binding site of protein‐ligand complexes and using the images for predicting the computational affinity information represented by pIC50 values. The CIFAP method has 2 phases, namely, data modeling and prediction. In data modeling phase, the separated 3D structure of the binding pocket with the ligand inside is fitted into an electrostatic potential grid box, which is then compressed through 3 orthogonal directions into three 2D images for each protein‐ligand complex. Sequential floating forward selection technique is performed for acquiring prediction patterns from the images. In the prediction phase, support vector regression (SVR) and partial least squares regression are used for testing the quality of the CIFAP method for predicting the binding affinity of 45 CHK1 inhibitors derived from 2‐aminothiazole‐4‐carboxamide. The results show that the CIFAP method using both support vector regression and partial least squares regression is very effective for predicting the binding affinities of CHK1‐ligand complexes with low‐error values and high correlation. As a future work, the results could be improved by working on the pose of the ligands inside the grid.
Development of a H2O2‐sensitive quantum dots‐based fluorescent sandwich ELISA for sensitive detection of bovine β‐lactoglobulin by monoclonal antibody
Authors: He Shengfa , Li Xin , Gao Jinyan , Tong Ping , Chen Hongbing 
Published: June 2017

AbstractBACKGROUNDBovine β‐lactoglobulin (BLG) is the major allergen in cows' milk, and the specific epitope plays a key role in food allergy. Developing a method specifically bind to the IgE epitope is necessary for testing BLG and its allergenic residues.RESULTSThe monoclonal antibody (1G9) specific to the IgE linear epitope for BLG was identified as high affinity and specificity. Based on 1G9, a sensitive fluorescent sandwich enzyme‐linked immunosorbent assay (sELISA) was successfully developed using catalase‐mediated fluorescence quenching of thiolated CdTe quantum dots in the presence of hydrogen peroxide as fluorescent signal output. The fluorescent sELISA showed high sensitivity and specificity, the limit of detection was 0.49 ng mL−1, which was 16‐fold lower than horseradish peroxidase (HRP)‐based sELISA. The linear range for BLG detection were 125–4000 ng mL−1 (r = 0.9939) and 0.48–62.5 ng mL−1 (r = 0.9919). The recoveries and coefficients of variation were 94.25–109.83% and 4.38–20.29%, respectively. Allergenic residues were also detected in hydrolysed infant formulas. The results of fluorescent sELISA showed good performance as HRP‐based sELISA and commercial sELISA kit.CONCLUSIONThis proposed fluorescent sELISA could be employed to detect BLG and its allergenic residues in food with highly sensitivity, reliability, and recovery. © 2017 Society of Chemical Industry
Characteristics improvement of top‐gate self‐aligned amorphous indium gallium zinc oxide thin‐film transistors using a dual‐gate control
Authors: Nag Manoj , De Roose Florian , Myny Kris , Steudel Soeren , Genoe Jan , Groeseneken Guido , Heremans Paul 
Published: June 2017

AbstractIn this work, we have reported dual‐gate amorphous indium gallium zinc oxide thin‐film transistors (a‐IGZO TFTs), where a top‐gate self‐aligned TFTs has a secondary bottom gate and the TFT integration comprises only five mask steps. The electrical characteristics of a‐IGZO TFTs under different gate control are compared. With the enhanced control of the channel with two gates connected together, parameters such as on current (ION), sub‐threshold slope (SS−1), output resistance, and bias‐stress instabilities are improved in comparison with single‐gate control self‐aligned a‐IGZO TFTs. We have also investigated the applicability of the dual‐gate a‐IGZO TFTs in logic circuitry such as 19‐stage ring oscillators.
Progress in the synthesis and biological evaluation of lipid A and its derivatives
Authors: Gao Jian , Guo Zhongwu 
Published: June 2017

AbstractLipid A is one of the core structures of bacterial lipopolysaccharides (LPSs), and it is mainly responsible for the strong immunostimulatory activities of LPS through interactions with the Toll‐like receptors and other molecules in the human immune system. To obtain structurally homogeneous and well‐defined lipid As and its derivatives in quantities meaningful for various biological studies and applications, their chemical synthesis has become a focal point. This review has provided a survey of significant progresses made in the synthesis of lipid A, and its derivatives that carry diverse saturated and unsaturated lipids, have the phosphate group at its reducing end replaced with a more stable phosphate or carboxyl group, or lack the reducing end phosphate or both phosphate groups, as well as progresses in the synthesis of LPS analogs and other lipid A conjugates. These synthetic molecules have facilitated the elucidation of the structure–activity relationships of lipid A useful for the design and development of lipid A based therapeutics, such as those utilized to treat sepsis, and other medical applications, for example the use of monophosphoryl lipid A as a carrier molecule for the study of fully synthetic self‐adjuvanting conjugate vaccines. These topics are also briefly covered in the current review.
Glypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment
Authors: Zhou Fubo , Shang Wenting , Yu Xiaoling , Tian Jie 
Published: June 2017

AbstractLiver cancer is the second leading cause of cancer‐related deaths, and hepatocellular carcinoma (HCC) is the most common type. Therefore, molecular targets are urgently required for the early detection of HCC and the development of novel therapeutic approaches. Glypican‐3 (GPC3), an oncofetal proteoglycan anchored to the cell membrane, is normally detected in the fetal liver but not in the healthy adult liver. However, in HCC patients, GPC3 is overexpressed at both the gene and protein levels, and its expression predicts a poor prognosis. Mechanistic studies have revealed that GPC3 functions in HCC progression by binding to molecules such as Wnt signaling proteins and growth factors. Moreover, GPC3 has been used as a target for molecular imaging and therapeutic intervention in HCC. To date, GPC3‐targeted magnetic resonance imaging, positron emission tomography, and near‐infrared imaging have been investigated for early HCC detection, and various immunotherapeutic protocols targeting GPC3 have been developed, including the use of humanized anti‐GPC3 cytotoxic antibodies, treatment with peptide/DNA vaccines, immunotoxin therapies, and genetic therapies. In this review, we summarize the current knowledge regarding the structure, function, and biology of GPC3 with a focus on its clinical potential as a diagnostic molecule and a therapeutic target in HCC immunotherapy.
Inequalities in uptake of prenatal screening according to ethnicity and socio‐economic status in the four largest cities of the Netherlands (2011–2013)
Authors: Posthumus Anke G. , Peters Ingrid A. , Borsboom Gerard J. , Knapen Maarten F. C. M. , Bonsel Gouke J. 
Published: June 2017

AbstractObjectivesIn the Netherlands, all women are claimed to have equal access to prenatal screening (PS). Prior research demonstrated substantial inequalities in PS uptake associated with socioeconomic status (SES) and ethnic background. The suggested pathway was a lack of intention to participate in PS among these subgroups. We studied the background of inequalities in PS participation, challenging intention heterogeneity as the single explanation.MethodsMultivariable logistic regression analyses of the national PS registry, focusing on the four largest cities in the Netherlands (n = 4578, years 2011–2013), stratified by SES. Outcome measures: (1) any uptake of PS (yes/no) and (2) uptake (one/two tests) for women who intended to participate in two tests. Determinants included intention, ethnicity, practice, and age.ResultsOf non‐Western women, 85.7% were screened versus 89.7% of Western women. Intention was an important explanatory factor in all models. However, after correction for intention, ethnicity remained a significant determinant for differences in uptake. Ethnicity and SES also interacted, indicating that non‐Western women in low SES areas had the lowest uptake (corrected for intention).Conclusions for PracticeSocioeconomic status and ethnicity related inequalities in PS uptake are only partially explained by intention heterogeneity; other pathways, in particular provider‐related determinants, may play a role. © 2017 John Wiley & Sons, Ltd.
Phosphine‐free direct arylation synthesis and self‐assembled nanostructure analysis of poly(3‐hexylselenophene)
Authors: Nakabayashi Kazuhiro , Otsuki Naoto , Mori Hideharu 
Published: June 2017

ABSTRACTPoly(3‐hexylselenophene)s (P3Hs) with high regioregularity (RR = 92–96%), that is, regioregular poly(3‐hexylselenophene)s (rr‐P3HSs), have been synthesized under the phosphine‐free direct arylation conditions in the presence of PdCl2 as a precatalyst. rr‐P3HS with the high molecular weight (Mn ∼ 10,000) was obtained as a result of screening of direct arylation conditions. Subsequently, the influences of primary structure, molecular weight (Mn = 3900–10,000) and regioregularity (RR = 57–96%), on optical properties and self‐assembled nanostructure of P3HS were investigated. X‐ray diffraction demonstrated that molecular weight, regioregularity, and preparation method of films dominate the crystallization behavior of P3HS. Among these parameters, it was evident that a high degree of regioregularity was the most fundamental contributor to achieve pure crystalline nanostructure. Furthermore, nanoassembly based on pure crystalline nanostructure, such as non‐woven fibrous and bundle‐like spherulitic self‐assembled nanostructures, was successfully prepared in rr‐P3HS, respectively, by appropriate modulation of the aforementioned parameters. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 2749–2755
Decentralized networked control of discrete‐time systems with local networks
Authors: Freirich Dror , Fridman Emilia 
Published: June 2017

SummaryThis paper considers discrete‐time large‐scale networked control systems with multiple local communication networks connecting sensors, controllers, and actuators. The local networks operate asynchronously and independently of each other in the presence of variable sampling intervals, transmission delays, and scheduling protocols (from sensors to controllers). The time‐delay approach that was recently suggested to decentralized stabilization of large‐scale networked systems in the continuous time is extended to
decentralized control in the discrete time. An appropriate Lyapunov‐Krasovskii method is presented that leads to efficient LMI conditions for the exponential stability and l2‐gain analysis of the closed loop large‐scale system. Differences from the continuous‐time results are discussed. A numerical example of decentralized control of 2 coupled cart‐pendulum systems illustrates the efficiency of the results.
Measuring the statistical validity of summary meta‐analysis and meta‐regression results for use in clinical practice
Authors: Willis Brian H. , Riley Richard D. 
Published: June 2017

An important question for clinicians appraising a meta‐analysis is: are the findings likely to be valid in their own practice—does the reported effect accurately represent the effect that would occur in their own clinical population? To this end we advance the concept of statistical validity—where the parameter being estimated equals the corresponding parameter for a new independent study. Using a simple (‘leave‐one‐out’) cross‐validation technique, we demonstrate how we may test meta‐analysis estimates for statistical validity using a new validation statistic, Vn, and derive its distribution.We compare this with the usual approach of investigating heterogeneity in meta‐analyses and demonstrate the link between statistical validity and homogeneity. Using a simulation study, the properties of Vn and the Q statistic are compared for univariate random effects meta‐analysis and a tailored meta‐regression model, where information from the setting (included as model covariates) is used to calibrate the summary estimate to the setting of application. Their properties are found to be similar when there are 50 studies or more, but for fewer studies Vn has greater power but a higher type 1 error rate than Q. The power and type 1 error rate of Vn are also shown to depend on the within‐study variance, between‐study variance, study sample size, and the number of studies in the meta‐analysis. Finally, we apply Vn to two published meta‐analyses and conclude that it usefully augments standard methods when deciding upon the likely validity of summary meta‐analysis estimates in clinical practice. © 2017 The Authors. Statistics in Medicine published by John Wiley & Sons Ltd.
Inside Cover Image, Volume 7, Issue 4
Authors: Ganesan Aravindhan , Coote Michelle L. , Barakat Khaled 
Published: June 2017

The inside cover image, by Aravindhan Ganesan et al., is based on the Overview Molecular ‘time‐machines’ to unravel key biological events for drug design, DOI: 10.1002/wcms.1306.
image
Energy scenarios: the value and limits of scenario analysis
Authors: Paltsev Sergey 
Published: June 2017

A need for low‐carbon world has added a new challenging dimension for the long‐term energy scenarios development. In addition to the traditional factors like technological progress, demographic, economic, political, and institutional considerations, there is another aspect of the modern energy forecasts related to the coverage, timing, and stringency of policies to mitigate the greenhouse gas emissions and air pollutants. Modern tools for the energy scenario development provide a good basis for the estimates of the required changes in the energy system to achieve certain climate and environmental targets. While the current scenarios show that a move to a low‐carbon energy future requires a drastic change in energy investment and the resulting mix in energy technologies, the exact technology mix, paths to the needed mix, price, and cost projections should be treated with a great degree of caution. The scenarios do not provide exact predictions, but they can be used as a qualitative analysis of decision‐making risks associated with different pathways. If history is any guide, energy scenarios overestimate the extent to which the future will look like the recent past. As future costs and the resulting technology mixes are uncertain, a wise government policy is to target emissions reductions from any source, rather than focus on boosting certain kinds of low‐carbon energy.WIREs Energy Environ2017, 6:e242. doi: 10.1002/wene.242This article is categorized under:Energy and Climate > Economics and PolicyEnergy and Climate > Systems and InfrastructureEnergy Policy and Planning > Economics and Policy
Gold nanoparticle‐mediated photothermal therapy: applications and opportunities for multimodal cancer treatment
Authors: Riley Rachel S. , Day Emily S. 
Published: June 2017

Photothermal therapy (PTT), in which nanoparticles embedded within tumors generate heat in response to exogenously applied laser light, has been well documented as an independent strategy for highly selective cancer treatment. Gold‐based nanoparticles are the main mediators of PTT because they offer: (1) biocompatibility, (2) small diameters that enable tumor penetration upon systemic delivery, (3) simple gold‐thiol bioconjugation chemistry for the attachment of desired molecules, (4) efficient light‐to‐heat conversion, and (5) the ability to be tuned to absorb near‐infrared light, which penetrates tissue more deeply than other wavelengths of light. In addition to acting as a standalone therapy, gold nanoparticle‐mediated PTT has recently been evaluated in combination with other therapies, such as chemotherapy, gene regulation, and immunotherapy, for enhanced anti‐tumor effects. When delivered independently, the therapeutic success of molecular agents is hindered by premature degradation, insufficient tumor delivery, and off‐target toxicity. PTT can overcome these limitations by enhancing tumor‐ or cell‐specific delivery of these agents or by sensitizing cancer cells to these additional therapies. All together, these benefits can enhance the therapeutic success of both PTT and the secondary treatment while lowering the required doses of the individual agents, leading to fewer off‐target effects. Given the benefits of combining gold nanoparticle‐mediated PTT with other treatment strategies, many exciting opportunities for multimodal cancer treatment are emerging that will ultimately lead to improved patient outcomes. WIREs Nanomed Nanobiotechnol 2017, 9:e1449. doi: 10.1002/wnan.1449This article is categorized under:

Therapeutic Approaches and Drug Discovery > Emerging Technologies
Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease
Preserving Relations in Parallel Flow Data Processing
Authors: Čejka Tomáš , Žádník Martin 
Published: June 2017

AbstractNetwork monitoring produces high volume of data that must be analyzed ideally in near real-time to support network security operations. It is possible to process the data using Big Data frameworks, however, such approach requires adaptation or complete redesign of processing tools to get the same results. This paper elaborates on a parallel processing based on splitting a stream of flow records. The goal is to create subsets of traffic that contain enough information for parallel anomaly detection. The paper describes a methodology based on so called witnesses that helps to scale up without any need to modify existing algorithms.