bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats
Authors: Micovic Zarko , Stamenkovic Aleksandra , Nikolic Tamara , Stojanovic Marija , Scepanovic Ljiljana , Hadzibegovic Adi , Obrenovic Radmila , Vujosevic Ivana , Stankovic Sanja , Djuric Marko , Jakovljevic Biljana , Djuric Dragan 
Published: June 2016

Abstract
Hyperhomocysteinemia (HHC), both basal and after methionine load, may occur due to genetic disorders or deficiencies of nutrients that affect the remethylation or trans-sulphuration pathways during methionine metabolism. HHC is involved in the pathogenesis of many illnesses as a result of its prooxidative effect and its impairment of antioxidative protection. The aim was to examine the effects of subchronic methionine overload on the body weight and standard biochemical parameters in rat serum and to examine whether simultaneous subchronic intraperotoneal administration of methionine alone or together with L-cysteine or N-acetyl-cysteine resulted in a change in the body weight and biochemical parameters in the rat serum. The research was conducted during a three-week period (male Wistar albino rats, n=36, body weight of approximately 160 g, age of 15-20 days), and the animals were divided into a control group and three experimental groups of 8-10 animals each: a) control group (0.9% sodium chloride 0.1-0.2 ml/day); b) methionine (0.8 mmol/kg/bw/day) (MET group); c) methionine (0.8 mmol/kg/bw/day) + L-cysteine (7 mg/kg/bw/day) (L-cys+MET group); and d) methionine (0.8 mmol/kg/bw/day) + N-acetyl-L-cysteine (50 mg/kg/bw/day) (NAC+MET group). In addition to the body weight monitoring, the levels of total homocysteine and the standard biochemical parameters in blood samples (plasma or serum) were determined. The results indicated that monitoring the homocysteine levels and standard biochemical parameters in blood could be used for analysis and could provide an excellent guideline for distinguishing between toxic and non-toxic doses of methionine intake, which may be meaningful for clinical applications.
Pneumothorax Related to Mechanical Ventilation: Silent Enemy
Authors: Mojsic Branislav , Mandras Ana , Sujica Maja , Vasiljevic Sladjana 
Published: June 2016

Abstract
Pneumothorax is well known and described complication in intensive care unit patients (ICU). Incidence of this complication is higher in patients with underlying pathology. As it can be occult, it is of the most importance to think of it in patients on mechanical ventilation. In this case report we well present ventilator-related pneumothorax in infant: clinical presentation, diagnosis and management
The Prevalence of Alcohol Consumption by Adolescents in Serbia and Its Correlation with Sociodemographic Factors – A National Survey
Authors: Radovanovic Snezana , Vasiljevic Dragan , Kocic Sanja , Radevic Svetlana , Milosavljević Mirjana , Mihailovic Nataša 
Published: June 2016

AbstractThe aim of this study was to determine the prevalence of alcohol consumption among adolescents in Serbia and its association with sociodemographic characteristics. This paper is based on data from a national health survey of the population of Serbia in 2013 (no data for Kosovo and Metohija), conducted by the Ministry of Health of the Republic of Serbia. For the purposes of this study, data on households and individuals over 15 years of age were used; thus, the final sample for analysis included 858 patients (aged 15 to 19 years). Researchers used demographic characteristics (age, gender, type of home, region) and socio-economic characteristics (income per household member, the index of well-being, self-assessment of health, cigarette smoking, tendency towards psychological and physical violence) as the independent variables. A χ2 test was applied to test the differences in the frequencies of categorical variables. The correlations between alcohol consumption, as the dependent variable, and the independent variables (mentioned above) were tested by logistic regression. All results less than or equal to 5% probability (p ≤ 0.05) were considered statistically significant. The prevalence of alcohol consumption among adolescents in Serbia is 51.6%. Alcohol consumption is significantly associated with sex, type of home and the index of well-being (p < 0.05). The prevalence of alcohol consumption is higher in males (57.1%), in adolescents who come from urban areas (59.3%) and in adolescents who, according to the index of well-being, belong to the wealthiest financial category (23.9%).
Plants from The Genus Daphne: A Review of its Traditional Uses, Phytochemistry, Biological and Pharmacological Activity
Authors: Sovrlić Miroslav M. , Manojlović Nedeljko T. 
Published: June 2016

AbstractPlants have an important role in maintaining people’s health and improving the quality of human life. They are an important component of people’s diet, but they are also used in other spheres of human life as a therapeutic resources, ingredients of cosmetic products, paints and others. The Daphne genus belongs to family Thymeleaceae which includes 44 families with approximately 500 herbal species. The plant species of the genus Daphne are used in the traditional medicine in China and tropical part of Africa for the treatment of various conditions. Previous studies showed significant biological potential of these species as a source of pharmacologically active compounds. This indicates that this genus possess a broad spectrum of biological activity including antimicrobial, antioxidant, analgesic, anti-inflammatory, cytotoxic, anti-ulcerogenic, abortive, hypocholesterolemic and hemostatic effects. Additionally, Daphne plants are the source of valuable bioactive phytochemicals such as coumarins, flavonoids, lignans, steroids and different classes of terpenes. Different parts of the Daphne plants contain specific bioactive metabolites and can represent a source of new, natural, pharmacologically active compounds, which may potentially be used in pharmaceutical, cosmetic and food industries.
Comparison of Biometric Values and Intraocular Lens Power Calculations Obtained by Ultrasound and Optical Biometry
Authors: Cvetkovic Aleksandra , Sreckovic Suncica , Petrovic Marko 
Published: June 2016

Abstract
This study sought to compare the biometric values and intraocular lens (IOL) power obtained by standard ultrasound and optical biometry.
We examined 29 eyes in preparation for cataract surgery. None of the patients had refractive surgery or corneal anomaly. In all patients, the horizontal and vertical refractive power of the cornea was determined using a keratometer (Bausch&Lomb). The axial length of the eye was determined via A-scan ultrasound (BVI-compact-V-plus) using Hollady’s formula. The IOL power and complete biometric measurements were obtained via an IOL Master-500-Zeiss using the Hollady-2 formula. All obtained values were compared and analysed using the statistical program SPSS 20.
The average age of treated patients was 71.21±1.68 years. In 16 patients with dense cataracts (55.17%), it was not possible to determine the IOL power by optical biometry. Optical biometry obtained significantly increased axial length values of 24.04±0.29 mm compared with those obtained with ultrasound biometry (23.89±0.28 mm, p=0.003). The mean refractive cornea power values of the horizontal meridian measured using a keratometer (42.50±0.47 D) and an IOL Master (42.69±0.49 D) were not statistically different (p=0.187). The mean values of the refractive cornea power of the vertical meridian obtained using a keratometer (42.62±0.48D) and an IOL Master (43.36±0.51 D) exhibited a statistically significant difference (p=0.000). The keratometer obtained statistically significant lower mean values of corneal refractive power (42.73±0.32 D) compared with those obtained with optical biometry (43.22±0.35 D, p=0.000). Ultrasound biometry obtained significantly increased the mean values of IOL power (20.19±0.48D) compared with those obtained with optical biometry (19.71±0.48 D, p=0.018).
The large number of patients who receive an operation for dense cataracts indicate the need for representation of both biometric methods in our clinical practice.
RAB2A controls MT1‐MMP endocytic and E‐cadherin polarized Golgi trafficking to promote invasive breast cancer programs
Authors: Kajiho Hiroaki , Kajiho Yuko , Frittoli Emanuela , Confalonieri Stefano , Bertalot Giovanni , Viale Giuseppe , Di Fiore Pier Paolo , Oldani Amanda , Garre Massimiliano , Beznoussenko Galina V , Palamidessi Andrea , Vecchi Manuela , Chavrier Philippe , Perez Frank , Scita Giorgio 
Published: June 2016

AbstractThe mechanisms of tumor cell dissemination and the contribution of membrane trafficking in this process are poorly understood. Through a functional siRNA screening of human RAB GTPases, we found that RAB2A, a protein essential for ER‐to‐Golgi transport, is critical in promoting proteolytic activity and 3D invasiveness of breast cancer (BC) cell lines. Remarkably, RAB2A is amplified and elevated in human BC and is a powerful and independent predictor of disease recurrence in BC patients. Mechanistically, RAB2A acts at two independent trafficking steps. Firstly, by interacting with VPS39, a key component of the late endosomal HOPS complex, it controls post‐endocytic trafficking of membrane‐bound MT1‐MMP, an essential metalloprotease for matrix remodeling and invasion. Secondly, it further regulates Golgi transport of E‐cadherin, ultimately controlling junctional stability, cell compaction, and tumor invasiveness. Thus, RAB2A is a novel trafficking determinant essential for regulation of a mesenchymal invasive program of BC dissemination.
Principles of microRNA Regulation Revealed Through Modeling microRNA Expression Quantitative Trait Loci
Authors: Budach Stefan , Heinig Matthias , Marsico Annalisa 
Published: June 2016

AbstractExtensive work has been dedicated to study mechanisms of microRNA-mediated gene regulation. However, the transcriptional regulation of microRNAs themselves is far less well understood, due to difficulties determining the transcription start sites of transient primary transcripts. This challenge can be addressed using expression quantitative trait loci (eQTLs) whose regulatory effects represent a natural source of perturbation of cis-regulatory elements. Here we used previously published cis-microRNA-eQTL data for the human GM12878 cell line, promoter predictions, and other functional annotations to determine the relationship between functional elements and microRNA regulation. We built a logistic regression model that classifies microRNA/SNP pairs into eQTLs or non-eQTLs with 85% accuracy; shows microRNA-eQTL enrichment for microRNA precursors, promoters, enhancers, and transcription factor binding sites; and depletion for repressed chromatin. Interestingly, although there is a large overlap between microRNA eQTLs and messenger RNA eQTLs of host genes, 74% of these shared eQTLs affect microRNA and host expression independently. Considering microRNA-only eQTLs we find a significant enrichment for intronic promoters, validating the existence of alternative promoters for intragenic microRNAs. Finally, in line with the GM12878 cell line derived from B cells, we find genome-wide association (GWA) variants associated to blood-related traits more likely to be microRNA eQTLs than random GWA and non-GWA variants, aiding the interpretation of GWA results.
Non-Tariff Barriers and Factors that influence The Indonesian Cocoa Export to Europe
Authors: Anggoro Raditya , Widyastutik Widyastutik 
Published: June 2016

The aim of this study are: first, to analyse the competitiveness of Indonesian cocoa in the European Unio market; second, to analyse the competitiveness and the factors that influence the Indonesian cocoa exports to the EU market; third, to calculate the non-tariff barriers imposed by the European Union market for Indonesian cocoa. Methods used are the RCA index and gravity models. The differences between actual and potential trade flows are indicated as non-tariff barriers. Results of the calculation RCA is showed that all destinations of a cocoa export have a high competitiveness (RCA> 1) but tend to decrease. Results of the estimate gravity models show the factors influencing the Indonesian cocoa exports are the real per capita GDP of Indonesia and the destination country, CPI of destination countries, the economic distance, exchange rates, and tariff. The result of non-tariff barriers indicated that the Netherlands is the state which has the largest non-tariff barriers among the most other EU countriesDOI: 10.15408/sjie.v5i1.3131
Two-Step Process for ED UTI Screening in Febrile Young Children: Reducing Catheterization Rates
Authors: Lavelle Jane M. , Blackstone Mercedes M. , Funari Mary Kate , Roper Christine , Lopez Patricia , Schast Aileen , Taylor April M. , Voorhis Catherine B. , Henien Mira , Shaw Kathy N. 
Published: June 2016

BACKGROUND AND OBJECTIVES:
Urinary tract infection (UTI) screening in febrile young children can be painful and time consuming. We implemented a screening protocol for UTI in a high-volume pediatric emergency department (ED) to reduce urethral catheterization, limiting catheterization to children with positive screens from urine bag specimens.

METHODS:
This quality-improvement initiative was implemented using 3 Plan-Do-Study-Act cycles, beginning with a small test of the proposed change in 1 ED area. To ensure appropriate patients received timely screening, care teams discussed patient risk factors and created patient-specific, appropriate procedures. The intervention was extended to the entire ED after providing education. Finally, visual cues were added into the electronic health record, and nursing scripts were developed to enlist family participation. A time-series design was used to study the impact of the 6-month intervention by using a p-chart to determine special cause variation. The primary outcome measure for the study was defined as the catheterization rate in febrile children ages 6 to 24 months.

RESULTS:
The ED reduced catheterization rates among febrile young children from 63% to &lt;30% over a 6-month period with sustained results. More than 350 patients were spared catheterization without prolonging ED length of stay. Additionally, there was no change in the revisit rate or missed UTIs among those followed within the hospital’s network.

CONCLUSIONS:
A 2-step less-invasive process for screening febrile young children for UTI can be instituted in a high-volume ED without increasing length of stay or missing cases of UTI.
Potato Virus Y (PVY) y Potato Yellow Vein Virus (PYVV) en Infecciones Mixtas No Causan Síntomas Atípicos en Plantas de Papa
Authors: Alfaro García Jenny Paola , Franco-Lara Liliana 
Published: June 2016

Observaciones anteriores en cultivos de papa (<em>Solanum tuberosum</em> y <em>Solanum phureja</em>) mostraron síntomas llamados atípicos en este trabajo, que consistían en hojas con manchas irregulares verde oscuro sobre un fondo amarillo intenso, que sugieren la presencia de virus. Estos síntomas no correlacionan con ningún virus descrito para papa en Colombia. En plantas, existen reportes de infecciones virales con dos o más virus que llevan a interacciones como sinergismo o antagonismo. En este trabajo se evaluó la hipótesis de que los síntomas atípicos podrían ser resultado de infecciones mixtas entre PYVV (<em>Potato yellow vein virus) (Crinivirus) </em>y PVY (<em>Potato virus Y</em>) (<em>Potyirus)</em>, pues ambos virus son comunes en cultivos de papa en Colombia. Se reportan resultados de RT-PCR (reacción en cadena de la polimerasa mediada por retrotranscripción) para la detección de PYVV y PVY, y de ELISA para PVX, PVS, PVM y PRLV en 57 plantas con y sin síntomas atípicos provenientes de campo. Los resultados no apoyan la hipótesis planteada, pues de 10 plantas con síntomas atípicos evaluadas solo una estaba infectada con los dos virus. Por otro lado, coinfecciones de PVY y PYVV se observaron en plantas sin síntomas aparentes (4 plantas de 5 evaluadas) y en plantas con síntomas característicos de PYVV (17 plantas de 37 evaluadas). Del total de 20 plantas evaluadas por ELISA, 18 presentaban infección por PVX aunque no se observaron síntomas asociados a este virus. Ocho de estas plantas, además de PVX estaban infectadas también con PVY y PYVV, pero mostraban síntomas de característicos de PYVV, lo que sugiere que PVX tampoco correlaciona con los síntomas atípicos. Se sugiere la presencia de un virus no reportado infectando el cultivo de papa en Colombia.
Efecto de Pyraclostrobin en la Producción de Papa Cultivar Diacol Capiro
Authors: Patiño Cardona Jennifer Andrea , Cotes Torres José Miguel , Ramírez González Jorge Andrés 
Published: June 2016

<p>La gota de la papa causada por <em>Phytophthora infestans </em>(Mont.) de Bary, es una enfermedad que causa grandes pérdidas al cultivo, y requiere una frecuente aplicación de productos químicos. El pyraclostrobin, tiene un modo de acción que se basa en la inhibición de la respiración mitocondrial para proteger de enfermedades fúngicas a diferentes cultivos y además a mostrado ser un producto que mejora la acumu­lación de asimilados en la planta. Este trabajo tuvo como objetivo evaluar el efecto bioestimulante del pyraclostrobin, para lo cual se aplicó el producto al tubérculo semilla y al follaje, evaluándose siete dife­rentes momentos de aplicación; se utilizó un testigo sin utilización de pyraclostrobin. Se encontró que, en general, son preferibles las aplicaciones solamente al follaje que al follaje y al tubérculo semilla. El pyraclostrobin aplicado al follaje en aporque y floración obtiene incrementos de materia seca superiores al control sin aplicación de 117%, 25% y 75% para hojas, tallos y tubérculos, respectivamente.</p><p> </p>
Hidromedusas del Pacífico Colombiano: Aspectos Básicos de su Dinámica Ecológica
Authors: Baldrich Chaparro Angela María , López Peralta Raúl Hernando 
Published: June 2016

<p>Este estudio examina la dinámica espacio-temporal de la comunidad de hidromedusas en el Océano Pacífico Colombiano (OPC) en relación con la temperatura, salinidad, biomasa mesozooplanctónica y variación día/noche en la superficie, usando algunos análisis estadísticos e índices ecológicos. Un total de 128 muestras de mesozooplancton fueron obtenidas mediante arrastres superficiales con una red cónica (50 cm de diámetro, malla de 363 μm provista de un flujómetro para estimar el volumen de agua filtrada) durante cinco cruceros (23 junio-12 julio 2001, 27 agosto-15 septiembre 2001, 03-22 septiembre 2002, 01-21 septiembre 2003 y 18 septiembre-08 octubre de 2004). Las densidades más altas se presentaron durante septiembre de 2002 y septiembre-octubre de 2004, quizás principalmente por la mayor oferta de alimento. Se identificaron nueve especies meroplanctónicas y nueve holoplanctónicas, cuyos ciclos de vida aún son desconocidos o poco comprendidos; las especies holoplanctónicas dominantes <em>Aglaura hemistoma, Liriope tetraphylla </em>y <em>Cytaeis etrastyla </em>fueron definidas como euritípicas en el OPC. La diversidad más alta (H´= 1,65) fue menor que en otras regiones de Pacífico americano. No se observó una secuencia clara en la aparición de especies exclusivas, características o generalistas, ni un patrón de distribución espacio-temporal, aunque si indicios de migración nocturna a aguas superficiales. La similaridad entre las estaciones se explicó por las variables abióticas o bióticas, más que por su ubicación costera, intermedia u oceánica, o por la hora de los registros. Esta aproximación ecológica preliminar refleja la complejidad ambiental del OPC y el escaso conocimiento del comportamiento de la comunidad de las hidromedusas en el OPC.</p>
Especies Arbóreas de las Familias Euphorbiaceae, Pittosporaceae y Salicaceae son Infectadas por ‘CA. Phytoplasma fraxini’ y ‘CA. Phytoplasma asteris’ en Infecciones Mixtas en Bogotá, Colombia
Authors: Perilla-Henao Laura M. , Franco-Lara Liliana 
Published: June 2016

<p class="Default"> La presencia de fitoplasmas del grupo 16SrI (<em>‘Ca. </em>Phytoplasma asteris’) fue reportada en <em>Croton </em>spp. (Eu­phorbiaceae), <em>Pittosporum undulatum </em>(Pittosporaceae) y <em>Populus nigra </em>(Salicaceae), en Bogotá. En este traba­jo se reporta la existencia adicional de fitoplasmas del grupo 16SrVII <em>‘Ca. </em>Phytoplasma fraxini’ en estas mismas especies de árboles ornamentales, por técnicas moleculares como PCR anidada, RFLP y secuenciación del gen 16SrRNA. Los resultados muestran la existencia de un complejo de fitoplasmas de los grupos 16SrI y 16SrVII que se asocian con síntomas como deformación general de la corona, ramas en copo, amarillamiento, elonga­ción anormal de brotes apicales, escobas de bruja y rebrotación epicórmica que afectan el estado de sanidad de los árboles. En diciembre de 2013 la prevalencia sintomática en <em>Croton </em>spp., <em>P. undulatum </em>y <em>P. nigra </em>y se estimó en 36%, 93% y 85% respectivamente. Este trabajo presenta evidencia de que plantas de familias dife­rentes a Oleaceae que son susceptibles a fitoplasmas del grupo 16SrVII y que en este caso se encuentran en infecciones mixtas con fitoplasmas del grupo 16SrI. Se presentan evidencias de una enfermedad emergente de alta prevalencia en estas especies de árboles han pasado desapercibidas hasta la fecha, pero suponen un riesgo para la supervivencia de los árboles urbanos en Bogotá.</p>
DETECÇÃO EM TEMPO REAL DA FREQUÊNCIA CARDÍACA DE PESSOAS POR MEIO DA ANÁLISE DE VARIAÇÕES TEMPORAIS EM VÍDEOS
Authors: Rodrigues Kessiler Almeida Silveira , Pereira Moisés Henrique Ramos , Pádua Flávio Luis Cardeal 
Published: June 2016

<p>As doenças cardiovasculares são, atualmente, as causas mais comuns de morbimortalidade no mundo. Na perspectiva da prevenção de doenças e agravos, tornam-se fundamentais ações que criem ambientes favoráveis à saúde e favoreçam escolhas saudáveis. Medidas de prevenção e monitoramento contínuo de sinais vitais são necessários, sendo a frequência cardíaca um sinal promissor. No entanto, tal monitoramento pode ser difícil e pouco eficiente, quando não impossível, em determinados casos, como por exemplo, vítimas de queimaduras. Este artigo propõe uma aplicação para monitoramento da frequência cardíaca não invasivo e sem a necessidade de contato, podendo ser manuseado por qualquer pessoa. Para a determinação da frequência cardíaca, a aplicação combina técnicas de processamento de imagens, tratamento de sinais fotopletismográficos e análise de variações temporais em vídeos. Os resultados obtidos demonstram que, considerando 95% de confiança estatística e um erro padrão de 1,08 batimentos por minuto, a aplicação desenvolvida possui a mesma média para aferições de batimentos cardíacos em relação a um dispositivo já consolidado no mercado para essa finalidade, mostrando-se como um método computacional promissor para medições em repouso.</p><p>Abstract </p><p>Cardiovascular diseases are currently the most common causes of morbidity and mortality worldwide. From the perspective of prevention of diseases and disorders, become fundamental actions that create supportive environments for health and promote healthy choices. Prevention and continuous monitoring of vital signs are necessary, and the heart rate a promising sign. However, such monitoring can be difficult and inefficient, if not impossible, in certain cases, such as burn victims. This paper proposes an application for monitoring heart rate non-invasive and without the need to touch and can be handled by anyone. For the determination of heart rate the application combines techniques of image processing, processing and analysis of signals photo-plethysmography temporal changes in video. The obtained results show that, considering a 95% statistical confidence and a standard error of 1.08 beats per minute, the developed application has the same average heartbeats' measurements in relation to a consolidated device on the market used for the same purpose, showing itself as a promising computational method for rest measurements.</p>