bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diverse Roles of Axonemal Dyneins in Drosophila Auditory Neuron Function and Mechanical Amplification in Hearing
Authors: Karak Somdatta , Jacobs Julie S. , Kittelmann Maike , Spalthoff Christian , Katana Radoslaw , Sivan-Loukianova Elena , Schon Michael A. , Kernan Maurice J. , Eberl Daniel F. , Göpfert Martin C. 
Published: November 2015

AbstractMuch like vertebrate hair cells, the chordotonal sensory neurons that mediate hearing inDrosophilaare motile and amplify the mechanical input of the ear. Because the neurons bear mechanosensory primary cilia whose microtubule axonemes display dynein arms, we hypothesized that their motility is powered by dyneins. Here, we describe two axonemal dynein proteins that are required forDrosophilaauditory neuron function, localize to their primary cilia and differently contribute to mechanical amplification in hearing. Promoter fusions revealed that the two axonemal dynein genes Dmdnah3(=CG17150) and Dmdnai2(=CG6053) are expressed in chordotonal neurons, including the auditory ones in the fly’s ear. Null alleles of both dyneins equally abolished electrical auditory neuron responses, yet whereas mutations in Dmdnah3facilitated mechanical amplification, amplification was abolished by mutations in Dmdnai2. Epistasis analysis revealed that Dmdnah3acts downstream of Nan-Iav channels in controlling the amplificatory gain. Dmdnai2, in addition to being required for amplification, was essential for outer dynein arms in auditory neuron cilia. This establishes diverse roles of axonemal dyneins inDrosophilaauditory neuron function and links auditory neuron motility to primary cilia and axonemal dyneins. Mutant defects in sperm competition suggest that both dyneins also function in sperm motility.
Decreased Diversity of the Oral Microbiota of Patients with Hepatitis B Virus-Induced Chronic Liver Disease: A Pilot Project
Authors: Ling Zongxin , Liu Xia , Cheng Yiwen , Jiang Xiawei , Jiang Haiyin , Wang Yuezhu , Li Lanjuan 
Published: November 2015

AbstractIncreasing evidence suggests that altered gut microbiota is implicated in the pathogenesis of hepatitis B virus-induced chronic liver disease (HBV-CLD). However, the structure and composition of the oral microbiota of patients with HBV-CLD remains unclear. High-throughput pyrosequencing showed that decreased oral bacterial diversity was found in patients with HBV-CLD. The Firmicutes/Bacteroidetes ratio was increased significantly, which indicated that dysbiosis of the oral microbiota participated in the process of HBV-CLD development. However, the changing patterns of the oral microbiota in patients with HBV-induced liver cirrhosis (LC) were almost similar to patients with chronic hepatitis B (CHB). HBV infection resulted in an increase in potential H2S- and CH3SH-producing phylotypes such as Fusobacterium, Filifactor, Eubacterium, Parvimonas and Treponema, which might contribute to the increased oral malodor. These key oral-derived phylotypes might invade into the gut as opportunistic pathogens and contribute to altering the composition of the gut microbiota. This study provided important clues that dysbiosis of the oral microbiota might be involved in the development of HBV-CLD. Greater understanding of the relationships between the dysbiosis of oral microbiota and the development of HBV-CLD might facilitate the development of non-invasive differential diagnostic procedures and targeted treatments of HBV-CLD patients harbouring specific oral phylotypes.
Aroma improvement by repeated freeze-thaw treatment during Tuber melanosporum fermentation
Authors: Xiao Deng-Rong , Liu Rui-Sang , He Long , Li Hong-Mei , Tang Ya-Ling , Liang Xin-Hua , Chen Tao , Tang Ya-Jie 
Published: November 2015

AbstractThe aroma attributes of sulfurous, mushroom and earthy are the most important characteristics of the aroma of Tuber melanosporum. However, these three aroma attributes are absent in the T. melanosporum fermentation system. To improve the quality of the aroma, repeated freeze-thaw treatment (RFTT) was adopted to affect the interplay of volatile organic compounds (VOCs). Using RFTT, not only was the score on the hedonic scale of the aroma increased from the “liked slightly” to the “liked moderately” grade, but the aroma attributes of sulfurous, mushroom and earthy could also be smelled in the T. melanosporum fermentation system for the first time. A total of 29 VOCs were identified and 9 compounds were identified as the key discriminative volatiles affected by RFTT. Amino acid analysis revealed that methionine, valine, serine, phenylalanine, isoleucine and threonine were the key substrates associated with the biosynthesis of the 9 key discriminative VOCs. This study noted that amino acid metabolism played an important role in the regulation of the aroma of the T. melanosporum fermentation system.
Anomalous Near-Surface Low-Salinity Pulses off the Central Oregon Coast
Authors: Mazzini Piero L. F. , Risien Craig M. , Barth John A. , Pierce Stephen D. , Erofeev Anatoli , Dever Edward P. , Kosro P. Michael , Levine Murray D. , Shearman R. Kipp , Vardaro Michael F. 
Published: November 2015

AbstractFrom mid-May to August 2011, extreme runoff in the Columbia River ranged from 14,000 to over 17,000 m3/s, more than two standard deviations above the mean for this period. The extreme runoff was the direct result of both melting of anomalously high snowpack and rainfall associated with the 2010–2011 La Niña. The effects of this increased freshwater discharge were observed off Newport, Oregon, 180 km south of the Columbia River mouth. Salinity values as low as 22, nine standard deviations below the climatological value for this period, were registered at the mid-shelf. Using a network of ocean observing sensors and platforms, it was possible to capture the onshore advection of the Columbia River plume from the mid-shelf, 20 km offshore, to the coast and eventually into Yaquina Bay (Newport) during a sustained wind reversal event. Increased freshwater delivery can influence coastal ocean ecosystems and delivery of offshore, river-influenced water may influence estuarine biogeochemistry.
Fluoride-containing podophyllum derivatives exhibit antitumor activities through enhancing mitochondrial apoptosis pathway by increasing the expression of caspase-9 in HeLa cells
Authors: Zhao Wei , Yang Yong , Zhang Ya-Xuan , Zhou Chen , Li Hong-Mei , Tang Ya-Ling , Liang Xin-Hua , Chen Tao , Tang Ya-Jie 
Published: November 2015

AbstractThis work aims to provide sampling of halogen-containing aniline podophyllum derivatives and their mode of action with an in-depth comparison among fluorine, chloride and bromide for clarifying the important role and impact of fluorine substitution on enhancing antitumor activity, with an emphasis on the development of drug rational design for antitumor drug. The tumor cytotoxicity of fluoride-containing aniline podophyllum derivatives were in general improved by 10–100 times than those of the chloride and bromide-containing aniline podophyllum derivatives since fluoride could not only strongly solvated in protic solvents but also forms tight ion pairs in most aprotic solvents. When compared with chloride and bromide, the higher electronegativity fluoride substituted derivatives significantly enhanced mitochondrial apoptosis pathway by remarkably increasing the expression of caspase-9 in HeLa cells. The current findings would stimulate an enormous amount of research directed toward exploiting novel leading compounds based on podophyllum derivatives, especially for the fluoride-substituted structures with promising antitumor activity.
Surveying the endomicrobiome and ectomicrobiome of bark beetles: The case of Dendroctonus simplex
Authors: Durand Audrey-Anne , Bergeron Amélie , Constant Philippe , Buffet Jean-Philippe , Déziel Eric , Guertin Claude 
Published: November 2015

AbstractMany bark beetles belonging to the Dendroctonus genus carry bacterial and fungal microbiota, forming a symbiotic complex that helps the insect to colonize the subcortical environment of the host tree. However, the biodiversity of those bacteria at the surface of the cuticle or inside the body parts of bark beetles is not well established. The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing. The ecto- and endomicrobiome and the subcortical galleries were investigated. Several bacterial genera were identified, among which Pseudomonas, Serratia and Yersinia are associated with the surface of the beetle cuticle and genera belonging to Enterobacteriaceae and Gammaproteobacteria with the interior of the insect body. The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups. These results suggest the presence of distinct bacterial microbiota on the surface of the cuticle and the interior of D. simplex body. Additionally, the bacterial diversity identified in the galleries is substantially different from the ectomicrobiome, which could indicate a selection by the insect. This study reports for the first time the identification of the eastern larch beetle microbiome.
Defining an additivity framework for mixture research in inducible whole-cell biosensors
Authors: Martin-Betancor K. , Ritz C. , Fernández-Piñas F. , Leganés F. , Rodea-Palomares I. 
Published: November 2015

AbstractA novel additivity framework for mixture effect modelling in the context of whole cell inducible biosensors has been mathematically developed and implemented in R. The proposed method is a multivariate extension of the effective dose (EDp) concept. Specifically, the extension accounts for differential maximal effects among analytes and response inhibition beyond the maximum permissive concentrations. This allows a multivariate extension of Loewe additivity, enabling direct application in a biphasic dose-response framework. The proposed additivity definition was validated and its applicability illustrated by studying the response of the cyanobacterial biosensor Synechococcus elongatus PCC 7942 pBG2120 to binary mixtures of Zn, Cu, Cd, Ag, Co and Hg. The novel method allowed by the first time to model complete dose-response profiles of an inducible whole cell biosensor to mixtures. In addition, the approach also allowed identification and quantification of departures from additivity (interactions) among analytes. The biosensor was found to respond in a near additive way to heavy metal mixtures except when Hg, Co and Ag were present, in which case strong interactions occurred. The method is a useful contribution for the whole cell biosensors discipline and related areas allowing to perform appropriate assessment of mixture effects in non-monotonic dose-response frameworks
SLCO1B1 Variants and Angiotensin Converting Enzyme Inhibitor (Enalapril) -Induced Cough: a Pharmacogenetic Study
Authors: Luo Jian-Quan , He Fa-Zhong , Wang Zhen-Min , Sun Ning-Ling , Wang Lu-Yan , Tang Gen-Fu , Liu Mou-Ze , Li Qing , Chen Xiao-Ping , Liu Zhao-Qian , Zhou Hong-Hao , Zhang Wei 
Published: November 2015

AbstractClinical observations suggest that incidence of cough in Chinese taking angiotensin converting enzyme inhibitors is much higher than other racial groups. Cough is the most common adverse reaction of enalapril. We investigate whether SLCO1B1 genetic polymorphisms, previously reported to be important determinants of inter-individual variability in enalapril pharmacokinetics, are associated with the enalapril-induced cough. A cohort of 450 patients with essential hypertension taking 10 mg enalapril maleate were genotyped for the functional SLCO1B1 variants, 388A > G (Asn130Asp, rs2306283) and 521T > C (Val174Ala, rs4149056). The primary endpoint was cough, which was recorded when participants were bothered by cough and respiratory symptoms during enalapril treatment without an identifiable cause. SLCO1B1 521C allele conferred a 2-fold relative risk of enalapril-induced cough (95% confidence interval [CI] = 1.34–3.04, P = 6.2 × 10−4) and haplotype analysis suggested the relative risk of cough was 6.94-fold (95% CI = 1.30–37.07, P = 0.020) in SLCO1B1*15/*15 carriers. Furthermore, there was strong evidence for a gene-dose effect (percent with cough in those with 0, 1, or 2 copy of the 521C allele: 28.2%, 42.5% and 71.4%, trend P = 6.6 × 10−4). Our study highlights, for the first time, SLCO1B1 variants are strongly associated with an increased risk of enalapril-induced cough. The findings will be useful to provide pharmacogenetic markers for enalapril treatment.
Characterizing and Exploring the Formation Mechanism of Salt Deposition by Reusing Advanced-softened, Silica-rich, Oilfield-produced Water (ASOW) in Superheated Steam Pipeline
Authors: Dong Bin , Xu Ying , Lin Senmin , Dai Xiaohu 
Published: November 2015

AbstractTo dispose of large volumes of oilfield-produced water, an environmentally friendly method that reuses advanced-softened, silica-rich, oilfield-produced water (ASOW) as feedwater was implemented via a 10-month pilot-scale test in oilfield. However, salt deposition detrimental to the efficiency and security of steam injection system was generated in superheated steam pipeline. To evaluate the method, the characteristics and formation mechanism of the deposition were explored. The silicon content and total hardness of the ASOW were 272.20 mg/L and 0.018 mg/L, respectively. Morphology and composition of the deposition were determined by scanning electron microscope–energy dispersive spectrometry (SEM-EDS), inductively coupled plasma–mass spectroscopy (ICP-MS), X-ray diffraction (XRD), laser Raman spectroscopy (LRS) and X-ray photoelectron spectroscopy (XPS). Na2Si2O5, Na2CO3and trace silanes were identified in the deposition. In addition, the solubility of the deposition was about 99%, suggesting that it is very different from traditional scaling. The results of a simulation experiment and thermal analysis system (TGA and TG-FTIR) proved that Na2CO3and Si(OH)4(gas) are involved in the formation of Na2Si2O5, which is ascribed mainly to the temperature difference between the superheated steam and the pipe wall. These findings provide an important reference for improving the reuse of ASOW and reducing its deposition.
Development and application of loop-mediated isothermal amplification for detecting the highly benzimidazole-resistant isolates in Sclerotinia sclerotiorum
Authors: Duan Ya Bing , Yang Ying , Wang Jian Xin , Liu Cong Chao , He Ling Ling , Zhou Ming Guo 
Published: November 2015

AbstractResistance of benzimidazole fungicides is related to the point mutation of the β-tubulin gene in Sclerotinia sclerotiorum. The point mutation at codon 198 (GAG → GCG, E198A) occurs in more than 90% of field resistant populations in China. Traditional detection methods of benzimidazole-resistant mutants of S. sclerotiorum are time-consuming, tedious and inefficient. To establish a suitable and rapid detection of benzimidazole-resistant mutants of S. sclerotiorum, an efficient and simple method with high specificity was developed based on loop-mediated isothermal amplification (LAMP). Eight sets of LAMP primers were designed and four sets were optimized to specially distinguish benzimidazole-resistant mutants of S. sclerotiorum. With the optimal LAMP primers, the concentration of LAMP components was optimized and the reaction conditions were set as 60–64 °C for 60 min. This method had a good specificity, sensitivity, stability and repeatability. In the 1491 sclerotia, 614 (41.18%) were positive by LAMP and 629 (42.19%) positive by MIC. Therefore, the LAMP assay is more feasible to detect benzimidazole-resistant mutants of S. sclerotiorum than traditional detection methods.
Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river
Authors: Atashgahi Siavash , Aydin Rozelin , Dimitrov Mauricio R. , Sipkema Detmer , Hamonts Kelly , Lahti Leo , Maphosa Farai , Kruse Thomas , Saccenti Edoardo , Springael Dirk , Dejonghe Winnie , Smidt Hauke 
Published: November 2015

AbstractThe impact of the installation of a technologically advanced wastewater treatment plant (WWTP) on the benthic microbial community of a vinyl chloride (VC) impacted eutrophic river was examined two years before and three and four years after installation of the WWTP. Reduced dissolved organic carbon and increased dissolved oxygen concentrations in surface water and reduced total organic carbon and total nitrogen content in the sediment were recorded in the post-WWTP samples. Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such asChloroflexiandFirmicutesin more oxic and nutrient poor post-WWTP sediments. Similarly, quantitative PCR analysis showed 1–3 orders of magnitude reduction in phylogenetic and functional genes of sulphate reducers, denitrifiers, ammonium oxidizers, methanogens and VC-respiringDehalococcoides mccartyi. In contrast, members ofProteobacteriaadapted to nutrient-poor conditions were enriched in post-WWTP samples. This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function. Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.
Actin polymerization is stimulated by actin cross-linking protein palladin
Authors: Gurung Ritu , Yadav Rahul , Brungardt Joseph G. , Orlova Albina , Egelman Edward H. , Beck Moriah R. 
Published: November 2015

The actin scaffold protein palladin regulates both normal cell migration and invasive cell motility, processes that require the co-ordinated regulation of actin dynamics. However, the potential effect of palladin on actin dynamics has remained elusive. In the present study, we show that the actin-binding immunoglobulin-like domain of palladin, which is directly responsible for both actin binding and bundling, also stimulates actin polymerization in vitro. Palladin eliminated the lag phase that is characteristic of the slow nucleation step of actin polymerization. Furthermore, palladin dramatically reduced depolymerization, slightly enhanced the elongation rate, and did not alter the critical concentration. Microscopy and in vitro cross-linking assays reveal differences in actin bundle architecture when palladin is incubated with actin before or after polymerization. These results suggest a model whereby palladin stimulates a polymerization-competent form of globular or monomeric actin (G-actin), akin to metal ions, either through charge neutralization or through conformational changes.
Efficacy of Multiple-Talker Phonetic Identification Training in Postlingually Deafened Cochlear Implant Listeners
Authors: Miller Sharon E. , Zhang Yang , Nelson Peggy B. 
Published: November 2015

Purpose
This study implemented a pretest-intervention-posttest design to examine whether multiple-talker identification training enhanced phonetic perception of the /ba/-/da/ and /wa/-/ja/ contrasts in adult listeners who were deafened postlingually and have cochlear implants (CIs).

Method
Nine CI recipients completed 8 hours of identification training using a custom-designed training package. Perception of speech produced by familiar talkers (talkers used during training) and unfamiliar talkers (talkers not used during training) was measured before and after training. Five additional untrained CI recipients completed identical pre- and posttests over the same time course as the trainees to control for procedural learning effects.

Results
Perception of the speech contrasts produced by the familiar talkers significantly improved for the trained CI listeners, and effects of perceptual learning transferred to unfamiliar talkers. Such training-induced significant changes were not observed in the control group.

Conclusion
The data provide initial evidence of the efficacy of the multiple-talker identification training paradigm for CI users who were deafened postlingually. This pattern of results is consistent with enhanced phonemic categorization of the trained speech sounds.
Vocal Parameters of Elderly Female Choir Singers
Authors: Aquino Fernanda , Ferreira Léslie 
Published: November 2015

Introduction Due to increased life expectancy among the population, studying the vocal parameters of the elderly is key to promoting vocal health in old age.
Objective This study aims to analyze the profile of the extension of speech of elderly female choristers, according to age group.
Method The study counted on the participation of 25 elderly female choristers from the Choir of Messianic Church of São Paulo, with ages varying between 63 and 82 years, and an average of 71 years (standard deviation of 5.22). The elders were divided into two groups: G1 aged 63 to 71 years and G2 aged 72 to 82. We asked that each participant count from 20 to 30 in weak, medium, strong, and very strong intensities. Their speech was registered by the software Vocalgrama that allows the evaluation of the profile of speech range. We then submitted the parameters of frequency and intensity to descriptive analysis, both in minimum and maximum levels, and range of spoken voice.
Results The average of minimum and maximum frequencies were respectively 134.82–349.96 Hz for G1 and 137.28–348.59 Hz for G2; the average for minimum and maximum intensities were respectively 40.28–95.50 dB for G1 and 40.63–94.35 dB for G2; the vocal range used in speech was 215.14 Hz for G1 and 211.30 Hz for G2.
Conclusion The minimum and maximum frequencies, maximum intensity, and vocal range presented differences in favor of the younger elder group.
Choice of Approach Does Not Affect Clinical and Radiologic Outcomes: A Comparative Cohort of Patients Having Anterior Lumbar Interbody Fusion and Patients Having Lateral Lumbar Interbody Fusion at 24 Months
Authors: Malham Gregory M. , Parker Rhiannon M. , Blecher Carl M. , Chow Fiona Y. , Seex Kevin A. 
Published: November 2015

Study Design Retrospective analysis of prospectively collected registry data. Objective This study aimed to compare the clinical and radiologic outcomes between comparative cohorts of patients having anterior lumbar interbody fusion (ALIF) and patients having lateral lumbar interbody fusion (LLIF). Methods Ninety consecutive patients were treated by a single surgeon with either ALIF ( n = 50) or LLIF ( n = 40). Inclusion criteria were patients age 45 to 70 years with degenerative disk disease or grade 1 to 2 spondylolisthesis and single-level pathology from L1 to S1. Patient-reported outcome measures included pain (visual analog scale), disability (Oswestry Disability Index [ODI]), and quality of life (Short Form 36 physical component score [PCS] and mental component scores [MCS]). Assessment of fusion and measurement of lordosis and posterior disk height were performed on computed tomography scans. Results At 24 months, patients having ALIF had significant improvements in back (64%) and leg (65%) pain and ODI (60%), PCS (44%), and MCS (26%; p < 0.05) scores. Patients having LLIF had significant improvements in back (56%) and leg (57%) pain and ODI (52%), PCS (48%), and MCS (12%; p < 0.05) scores. Fourteen complications occurred in the ALIF group, and in the LLIF group, there were 17 complications ( p > 0.05). The fusion rate was 100% for ALIF and 95% for LLIF ( p = 0.1948). ALIF added ∼6 degrees of lordosis and 3 mm of height, primarily measured at L5–S1, and LLIF added ∼3 degrees of lordosis and 2 mm of height between L1 to L5. Mean follow-up was 34.1 months. Conclusions In comparative cohorts of patients having ALIF and patients having LLIF at 24 months postoperatively, there were no significant differences in clinical outcomes, complication rates, or fusion rates.