bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal changes in the structure and inflammatory response of the intervertebral disc due to stab injury in a murine organ culture model
Authors: Abraham Adam C. , Liu Jennifer W. , Tang Simon Y. 
Published: June 2016

ABSTRACTDespite the significant public health impact of intervertebral disc (IVD) degeneration and low back pain, it remains challenging to investigate the multifactorial molecular mechanisms that drive the degenerative cascade. Organ culture model systems offer the advantage of allowing cells to live and interact with their native extracellular matrix, while simultaneously reducing the amount of biological variation and complexity present at the organismal level. Murine organ cultures in particular also allow the use of widely available genetically modified animals with molecular level reporters that would reveal insights on the degenerative cascade. Here, we utilize an organ culture system of murine lumbar functional spinal units where we are able to maintain the cellular, metabolic, and structural, and mechanical stability of the whole organ over a 21‐day period. Furthermore, we describe a novel approach in organ culture by using tissues from animals with an NF‐κB‐luc reporter in combination with a mechanical injury model, and are able to show that proinflammatory factors and cytokines such as NF‐κB and IL‐6 produced by IVD cells can be monitored longitudinally during culture in a stab injury model. Taken together, we utilize a murine organ culture system that maintains the cellular and tissue level behavior of the intervertebral disc and apply it to transgenic animals that allow the monitoring of the inflammatory profile of IVDs. This approach could provide important insights on the molecular and metabolic mediators that regulate the homeostasis of the IVD. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1431–1438, 2016.
Response surface methodology applied to the generation of casein hydrolysates with antioxidant and dipeptidyl peptidase IV inhibitory properties
Authors: Nongonierma Alice B , Maux Solène Le , Esteveny Claire , FitzGerald Richard J 
Published: June 2016

AbstractBACKGROUNDHydrolysis parameters affecting the release of dipeptidyl peptidase IV (DPP‐IV) inhibitory and antioxidant peptides from milk proteins have not been extensively studied. Therefore, a multifactorial (i.e. pH, temperature and hydrolysis time) composite design was used to optimise the release of bioactive peptides (BAPs) with DPP‐IV inhibitory and antioxidant [oxygen radical absorbance capacity (ORAC)] properties from sodium caseinate.RESULTSFifteen sodium caseinate hydrolysates (H1–H15) were generated with ProtamexTM, a bacillus proteinase activity. Hydrolysis time (1 to 5 h) had the highest influence on both DPP‐IV inhibitory properties and ORAC activity (P < 0.05). Alteration of incubation temperature (40 to 60 °C) and pH (6.5 to 8.0) had an effect on the DPP‐IV inhibitory properties but not the ORAC activity of the Protamex sodium caseinate hydrolysates. A multi‐functional hydrolysate, H12, was identified having DPP‐IV inhibitory (actual: 0.82 ± 0.24 vs. predicted optimum: 0.68 mg mL−1) and ORAC (actual: 639 ± 66 vs. predicted optimum: 639 µmol TE g−1) activity of the same order (P > 0.05) as the response surface methodology (RSM) predicted optimum bioactivities.CONCLUSIONGeneration of milk protein hydrolysates through multifactorial design approaches may aid in the optimal enzymatic release of BAPs with serum glucose lowering and antioxidant properties. © 2016 Society of Chemical Industry
Design considerations for highly efficient edge‐lit quantum dot displays
Authors: Twietmeyer Karen , Sadasivan Sridhar 
Published: June 2016

AbstractQuantum dots (QDs) are increasingly the technology of choice for wide color gamut displays. Two popular options to incorporate QDs into displays include on‐edge and on‐surface solutions. The opto‐mechanical design for an on‐edge QD solution including a LED light bar (“on‐edge QD light bar”) is more complex than the design for a standard white phosphor LED light bar. In this paper, we identify and investigate a range of design parameters for an on‐edge QD light bar, and we show that these parameters have significant influence on system efficiency and color uniformity. The effects of varying these parameters are explored through the use of a custom adjustable testbed and optical raytracing methods. Our testbed data demonstrate the inherent trade‐offs between efficiency and color uniformity and provide guidance for the design of high‐performing displays. The optical raytracing data demonstrate a good predictive capability and support the use of optical modeling methods for a detailed exploration of a wider range of design parameters.
The Effects of Trauma History and Prenatal Affective Symptoms on Obstetric Outcomes
Authors: Blackmore Emma Robertson , Putnam Frank W. , Pressman Eva K. , Rubinow David R. , Putnam Karen T. , Matthieu Monica M. , Gilchrist Michelle A. , Jones Ian , O'Connor Thomas G. 
Published: June 2016

AbstractPrenatal maternal mood may inform the adverse obstetric outcomes seen in disadvantaged populations. The contribution of having a trauma history is not well studied. We examined the impact of trauma exposure and mood symptoms on obstetric outcomes in 358 women. Women with antecedent trauma were more likely to have a history of depression, odds ratio = 2.83, 95% confidence interval [1.81, 4.42], were younger at their first pregnancy, 18.86 years versus 20.10 years, and had a higher number of previous pregnancies, 2.01 versus 1.54, compared to those with no trauma exposure. Women with prenatal anxiety had significantly smaller babies than nonanxious women, 3,313.17 g, (SD = 441.58) versus 3,429.27 g, (SD = 437.82) Trauma history magnified the effects of maternal prenatal mood on birthweight; the moderating effect was limited to those who first experienced a trauma under 18 years of age. Childhood trauma exposure increased vulnerability for low birthweight delivery associated with prenatal mood disturbance. Screening pregnant women for trauma history and current mood symptoms is indicated.
Three‐dimensional Luneburg lens at optical frequencies
Authors: Zhao Yuan‐Yuan , Zhang Yong‐Liang , Zheng Mei‐Ling , Dong Xian‐Zi , Duan Xuan‐Ming , Zhao Zhen‐Sheng 
Published: June 2016

AbstractA Luneburg lens is a fascinating gradient refractive index (GRIN) lens that can focus parallel light on a perfect point without aberration in geometrical optics. Constructing a three‐dimensional (3D) Luneburg lens at optical frequencies is a challenging task due to the difficulty of fabricating the desired GRIN materials. Here, we present the practical implementation of a 3D Luneburg lens at optical frequencies. Such a 3D Luneburg lens is designed with GRIN 3D simple cubic metamaterial structures, and fabricated with dielectric metamaterials by femtosecond laser direct writing in the commercial negative‐photoresist IP‐L. Simulated and experimental results exhibit an interesting 3D ideal focus for the infrared light. The protocol for developing the 3D Luneburg lens with ideal focus would prompt the potential applications in integrated light‐coupled devices and lab‐on‐chip integrated biological sensors based on infrared light.
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Programmable Oil/Water Separation Meshes: Water or Oil Selectivity Using Contact Angle Hysteresis
Authors: Dunderdale Gary J. , England Matt W. , Sato Tomoya , Urata Chihiro , Hozumi Atsushi 
Published: June 2016

We report a novel method for oil/water separation using stainless steel meshes functionalized with amphiphilic copolymer, poly(methacrylic acid‐co‐ethylhexylmethacrylate) (PMAA‐co‐EHMA), brushes. Because the PMAA‐co‐EHMA brush‐covered surfaces show a large contact angle hysteresis at the oil/water contact line, the meshes can be programmed to act as either water‐selective or oil‐selective filters simply by pre‐wetting the mesh with one of these liquids. These programmable meshes can separate oil/water mixtures to high filtrate purities (more than 99 % mol/mol) in both oil‐selective and water‐selective modes.image
Super‐toughened poly(l‐lactic acid) fabricated via reactive blending and interfacial compatibilization
Authors: Ma Meng , Zheng Haiming , Chen Si , Wu Bozhen , He Huiwen , Chen Liqing , Wang Xu 
Published: June 2016

AbstractSuper‐toughened poly(l‐lactic acid) (PLLA) was prepared by reactive blending of PLLA with poly(ϵ‐caprolactone) (PCL), glycerol and 4,4′‐methylenediphenyl diisocyanate. The reactive interfacial compatibility between PLLA and the formed crosslinked polyurethane (CPU) in the PLLA matrix was studied in detail. The morphology and the toughness of the blends can be tuned by changing the CPU content. The results indicate that the impact strength of PLLA shows a tendency to higher values with the increasing PCL content up to 20 wt%. The notched impact strength of the blend with 20 wt% PCL increases to 55.01 kJ m−2, which is 24.9 times higher than that of neat PLLA. The elongation at break is also increased from 5% to 139.4%, indicating the brittle − ductile transition. The increased interfacial binding strength through the reactive interfacial compatibility and the formation of a CPU network in the PLLA matrix account for the improved toughness of PLLA/CPU blends. Dynamic mechanical analysis results indicate that the compatibility between PLLA and CPU is improved with increasing CPU content resulting in the formation of more interfacial phase. In addition, rheological property measurements indicate that the improvement in storage modulus and complex viscosity is ascribed to the formation of a CPU network in the PLLA matrix. © 2016 Society of Chemical Industry
The interplay between effector binding and allostery in an engineered protein switch
Authors: Choi Jay H. , Xiong Tina , Ostermeier Marc 
Published: June 2016

AbstractThe protein design rules for engineering allosteric regulation are not well understood. A fundamental understanding of the determinants of ligand binding in an allosteric context could facilitate the design and construction of versatile protein switches and biosensors. Here, we conducted extensive in vitro and in vivo characterization of the effects of 285 unique point mutations at 15 residues in the maltose‐binding pocket of the maltose‐activated β‐lactamase MBP317‐347. MBP317‐347 is an allosteric enzyme formed by the insertion of TEM‐1 β‐lactamase into the E. coli maltose binding protein (MBP). We find that the maltose‐dependent resistance to ampicillin conferred to the cells by the MBP317‐347 switch gene (the switch phenotype) is very robust to mutations, with most mutations slightly improving the switch phenotype. We identified 15 mutations that improved switch performance from twofold to 22‐fold, primarily by decreasing the catalytic activity in the absence of maltose, perhaps by disrupting interactions that cause a small fraction of MBP in solution to exist in a partially closed state in the absence of maltose. Other notable mutations include K15D and K15H that increased maltose affinity 30‐fold and Y155K and Y155R that compromised switching by diminishing the ability of maltose to increase catalytic activity. The data also provided insights into normal MBP physiology, as select mutations at D14, W62, and F156 retained high maltose affinity but abolished the switch's ability to substitute for MBP in the transport of maltose into the cell. The results reveal the complex relationship between ligand binding and allostery in this engineered switch.
Network representation of protein interactions—Experimental results
Authors: Kurzbach Dennis , Flamm Andrea G. , Sára Tomáš 
Published: June 2016

AbstractA graph theoretical analysis of nuclear magnetic resonance (NMR) data of six different protein interactions has been presented. The representation of the protein interaction data as a graph or network reveals that all of the studied interactions are based on a common functional concept. They all involve a single densely packed hub of functionally correlated residues that mediate the ligand binding events. This is found independent of the kind of protein (folded or unfolded) or ligand (protein, polymer or small molecule). Furthermore, the power of the graph analysis is demonstrated at the examples of the Calmodulin (CaM)/Calcium and the Cold Shock Protein A (CspA)/RNA interaction. The presented approach enables the precise determination of multiple binding sites for the respective ligand molecules.
Novel Gene Expression Signature Predictive of Clinical Recurrence After Radical Prostatectomy in Early Stage Prostate Cancer Patients
Authors: Shahabi Ahva , Lewinger Juan Pablo , Ren Jie , April Craig , Sherrod Andy E. , Hacia Joseph G. , Daneshmand Siamak , Gill Inderbir , Pinski Jacek K. , Fan Jian‐Bing , Stern Mariana C. 
Published: June 2016

BACKGROUNDCurrent clinical tools have limited accuracy in differentiating patients with localized prostate cancer who are at risk of recurrence from patients with indolent disease. We aimed to identify a gene expression signature that jointly with clinical variables could improve upon the prediction of clinical recurrence after RP for patients with stage T2 PCa.METHODSThe study population includes consented patients who underwent a radical retropubic prostatectomy (RP) and bilateral pelvic lymph node dissection at the University of Southern California in the PSA‐era (1988–2008). We used a nested case‐control study of 187 organ‐confined patients (pT2N0M0): 154 with no recurrence (“controls”) and 33 with clinical recurrence (“cases”). RNA was obtained from laser capture microdissected malignant glands representative of the overall Gleason score of each patient. Whole genome gene expression profiles (29,000 transcripts) were obtained using the Whole Genome DASL HT platform (Illumina, Inc). A gene expression signature of PCa clinical recurrence was identified using stability selection with elastic net regularized logistic regression. Three existing datasets generated with the Affymetrix Human Exon 1.0ST array were used for validation: Mayo Clinic (MC, n = 545), Memorial Sloan Kettering Cancer Center (SKCC, n = 150), and Erasmus Medical Center (EMC, n = 48). The areas under the ROC curve (AUCs) were obtained using repeated fivefold cross‐validation.RESULTSA 28‐gene expression signature was identified that jointly with key clinical variables (age, Gleason score, pre‐operative PSA level, and operation year) was predictive of clinical recurrence (AUC of clinical variables only was 0.67, AUC of clinical variables, and 28‐gene signature was 0.99). The AUC of this gene signature fitted in each of the external datasets jointly with clinical variables was 0.75 (0.72–0.77) (MC), 0.90 (0.86–0.94) (MSKCC), and 0.82 (0.74–0.91) (EMC), whereas the AUC for clinical variables only in each dataset was 0.72 (0.70–0.74), 0.86 (0.82–0.91), and 0.76 (0.67–0.85), respectively.CONCLUSIONSWe report a novel gene‐expression based classifier identified using agnostic approaches from whole genome expression profiles that can improve upon the accuracy of clinical indicators to stratify early stage localized patients at risk of clinical recurrence after RP.Prostate 76:1239–1256, 2016. © 2016 Wiley Periodicals, Inc.
σ‐algebras for quasirandom hypergraphs
Authors: Towsner Henry 
Published: June 2016

AbstractWe examine the correspondence between the various notions of quasirandomness for k‐uniform hypergraphs and σ‐algebras related to measurable hypergraphs. This gives a uniform formulation of most of the notions of quasirandomness for dense hypergraphs which have been studied, with each notion of quasirandomness corresponding to a σ‐algebra defined by a collection of subsets of .We associate each notion of quasirandomness with a collection of hypergraphs, the ‐adapted hypergraphs, so that G is quasirandom exactly when it contains roughly the correct number of copies of each ‐adapted hypergraph. We then identify, for each , a particular ‐adapted hypergraph with the property that if G contains roughly the correct number of copies of then G is quasirandom in the sense of . This generalizes recent results of Kohayakawa, Nagle, Rödl, and Schacht; Conlon, Hàn, Person, and Schacht; and Lenz and Mubayi giving this result for some particular notions of quasirandomness. © 2016 Wiley Periodicals, Inc. Random Struct. Alg., 50, 114–139, 2017
Scaling Law of Exciton Properties in the Surface Hydrogenated Armchair Graphene Nanoribbon
Authors: Zhu Xi , Wang Min 
Published: June 2016

AbstractThe scaling law of exciton effect in the surface hydrogenated graphene nanoribbon with armchair edge (H‐AGNRs) is studied by first principle density function theory (DFT) with quasi‐particle corrections and many‐body, i. e. electron‐hole interactions. Realizing the failure of the traditional family effect in the pristine AGNRs, we introduce an effective width model, which can systematically describe the electronic structures of H‐AGNRs by three catalogues. In the model, the relationship between the effective widths and the effective family index can well line up at the ground states of H‐AGNRs. We also scale the exciton binding energies with effective width of H‐AGNRs. This work provides more general understanding for the surface modification on the electronic properties in ultra‐narrow AGNRs and discloses a more universal family effect in the AGNRs systems.
Guest‐Induced, Self‐Assembled Supramolecular Capsule: Effect of Guest and Counter Anions
Authors: Satha Mr. Pardhasaradhi , Illa Mr. Giriteja , Ghosh Arindam , Purohit Chandra Shekhar 
Published: June 2016

AbstractMolecular capsules form due to various interactions between molecules. Cyclotricatechylene (CTC) is a poly‐phenolic macrocycle that has been shown to form capsule under highly basic conditions. Here, we describe the formation of a molecular capsule under ambient conditions, from CTC by addition of a guest molecule. The capsule is most probably forming through pure electrostatic interaction between the guest and CTC. The capsule formation has been studied using solution techniques such as NMR and Mass spectroscopy. The information about capsule formation is also obtained from the solid state structure using single crystal X‐ray diffraction studies, which corroborates well with the findings in solution state. Upon changing the cation to a larger one, both capsule formation and the encapsulation are hindered. However, change of anions keeping the cation unchanged does not affect the assembly process.
Folded Structure Stabilized by C7, C10 and C12 Hydrogen Bonds in αγ Hybrid Peptides
Authors: Wani Naiem Ahmad , Gupta Vivek Kumar , Singh Umesh Prasad , Aravinda Subrayashastry , Rai Rajkishor 
Published: June 2016

AbstractThe present work describes the synthesis and crystallographic analysis of αγ hybrid peptides, Boc‐Gpn‐D‐Pro‐NHMe (P1), Boc‐Aib‐Gpn‐D‐Pro‐NHMe (P2) and Boc‐Gpn‐D‐Pro‐Aib‐NHMe (P3). Aib, Gpn and Pro refer to α‐aminoisobutyric acid, gabapentin and proline, respectively. Peptides P1 and P2 adopt expanded twelve membered (C12) helical left‐handed turn over γα segment. Peptide P3 adopts folded conformation stabilized by three‐centre, double hydrogen bond motif, seven membered (C7) followed by type II′ β‐turn (C10). Aib residue adopts left handed helical conformation in P2, while right handed helical conformation is observed for it in peptide P3.
Gastropods from the Carboniferous (Namurian) of Congleton Edge, Cheshire,UK
Authors: Peel John S. 
Published: June 2016

AbstractGastropod assemblages of late Carboniferous age (Namurian; Chokierian–Alportian) from the Morridge Formation at Pot Bank Quarry, Congleton Edge, Cheshire, contain 27 species, the most diverse gastropod fauna known from the Silesian of the southernUK. Chokierian sandstones yield a sparse assemblage of mainly high‐spiredStegocoeliaand bivalves, but flooding associated with the Hudsonoceras proteus marine band (basal Alportian) introduced a diverse invertebrate fauna in which the gastropod fraction is dominated by the bellerophontoideansBellerophon,Retispira,EuphemitesandPatellilabia, and the eotomarioidAngyomphalus. A later, previously unrecognized marine assemblage of probable Alportian age contains numerous small pleurotomariiform gastropods, but bellerophontoideans are less common andAngyomphalusis absent. In formal international usage the assemblages are of Pennsylvanian (Bashkirian Stage) age. One new genusLiraloron cornoviorumgen. et sp. nov. and the following new species are described:Retispira mowensis,Patellilabia britannica,Angyomphalus congletonensis,Neilsonia coatesi,N. ganneyica,Eirlysia ceramicorum,Meekospira acrolopha.
3D Luminescent Copper(I) Iodide Coordination Polymer Based on Cu4I4 Clusters and an Ethyl‐bridging Bis(triazole) Ligand
Authors: Dou Ai‐Na , Du Yuan‐Chao , Chen Qiu‐Lu , Luo Kai‐Li , Zhang Ce , Zhu Ai‐Xin , Li Qiu‐Xia 
Published: June 2016

AbstractThe copper‐iodine based coordination polymer [Cu4I2(bmte)]n (1) [H2bmte = 1,2‐bis(5‐methyl‐1H‐1,2,4‐triazol‐3‐yl)ethane] was synthesized using cuprous iodide and a flexible 3‐substituted, ethyl‐bridging bis(triazole) ligand under solvothermal conditions. X‐ray diffraction analysis reveals that compound 1 shows a 3D framework containing Cu4I4 clusters and alternating left‐ and right‐handed [Cu(triazole)] helices, which result in a (4,8)‐connected fluorite (flu) topological network. Moreover, compound 1 exhibits orange phosphorescence with the emission maxima at 590 nm in the solid state at room temperature.
Hexaaza‐dithia Macrocycles Containing Pendant Methoxyethyl Groups: Synthesis and Characterization of Mono and Dinuclear Nickel Complexes
Authors: Gressenbuch Mathias , Kersting Berthold 
Published: June 2016

AbstractThe deprotection of two bicyclic hexaaza‐dithioether macrocycles 1 and 2 containing four (or six) N‐bound 2‐methoxyethyl substituents was achieved in good yields utilizing Li or Na/naphthalene in THF as the reagent. The ability of the resulting hexaaza‐dithiophenolate macrocycles (H2L4 and H2L5) to act as mono‐ and dinucleating ligands is also demonstrated. Three nickel complexes, namely [Ni(H2L4)][BPh4]2 (3), [Ni2(L4)(μ‐OAc)][BPh4] (4), and [Ni2(L5)(μ‐OAc)][BPh4] (5), were obtained and characterized by elemental analyses, ESI mass spectrometry, IR and UV/Vis spectroscopy, and in case of 3 and 4 also by X‐ray crystallography. The five‐coordinate nickel atom in complex 3 was found to interact only weakly with one of the four 2‐methoxyethyl groups. The nickel atoms in dinuclear 4 and 5 are coordinatively saturated by the nitrogen and sulfur donor atoms of the macrocycle and the oxygen atoms of the bridging acetato coligands, such that the methoxyethyl substituents function only as dangling groups.