bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increasing Colorectal Cancer Screening Using a Quality Improvement Approach in a Nurse-Managed Primary Care Clinic
Authors: Hountz Diane , Coddington Jennifer , Foli Karen J. , Thorlton Janet 
Published: October 2017

Abstract:
According to the American Cancer Society, 1 in 23 Americans will be diagnosed with colorectal cancer (CRC) in their lifetime. Screening for CRC is an effective, yet underused preventive approach. This is especially true in rural areas, where only 35% of patients were found to be up to date on their screenings in 2014. Increasing CRC screening can produce positive patient outcomes by early recognition and removal of precancerous polyps. The purpose of this project was to use quality improvement (QI) interventions to increase CRC screening rates at a nurse-managed clinic in rural Indiana. Using Deming's Plan-Do-Study-Act QI model, multiple interventions were implemented which resulted in a 37% increase in the number of screenings ordered on eligible patients and an overall increase of 28% in the completion of the screenings. This project contributes to healthcare quality knowledge by also suggesting that the fundamental principles of encouraging staff feedback to gain buy-in, improving processes informed by patient data, and valuing frequent performance feedback to staff, strengthened this QI project and ensured adoption and sustainability of these results.
Clinical Aspects of Miscarriage
Authors: Walter Marie A. , Alvarado Mary S. 
Published: October 2017

Abstract
It is estimated that 750,000 to 1,000,000 miscarriages occur every year in the United States. Women experiencing a miscarriage enter the healthcare system in a variety of ways. A family may be seen for a miscarriage in the prenatal clinic, the emergency department, same-day surgical department, or perhaps the labor and birth unit. Nurses must be prepared to guide and support these families. Understanding the clinical aspects of miscarriage as well as the emotional care of families experiencing early pregnancy loss is important to nurses in all areas of the medical center. Clinical aspects of miscarriage are reviewed along with the needed emotional care for families experiencing the most common cause of early pregnancy loss.
Vagus nerve stimulation inhibits trigeminal nociception in a rodent model of episodic migraine
Authors: Hawkins Jordan L. , Cornelison Lauren E. , Blankenship Brian A. , Durham Paul L. 
Published: October 2017

Abstract

Introduction:
Although neck muscle tension is considered a risk factor for migraine, pungent odors can act as a trigger to initiate an attack in sensitized individuals. Although noninvasive vagus nerve stimulation (nVNS) is now an approved treatment for chronic migraine, how it functions to inhibit trigeminal nociception in an episodic migraine model is not known.

Objectives:
The objectives of this study were to determine if nVNS could inhibit trigeminal nociception in a novel model of episodic migraine and investigate changes in the expression of proteins implicated in peripheral and central sensitization.

Methods:
Sprague-Dawley male rats were injected with an inflammatory agent in the trapezius muscle before exposure to pungent volatile compounds, which was used to initiate trigeminal nociceptor activation. The vagus nerve was stimulated transdermally by a 1-ms pulse of 5 kHz sine waves, repeated at 25 Hz for 2 minutes. Nocifensive head withdrawal response to von Frey filaments was determined and immunoreactive protein levels in the spinal cord and trigeminal ganglion (TG) were investigated.

Results:
Exposure to the pungent odor significantly increased the number of nocifensive withdrawals in response to mechanical stimulation of sensitized TG neurons mediated by neck muscle inflammation. Noninvasive vagus nerve stimulation inhibited nociception and repressed elevated levels of P-ERK in TG, Iba1 in microglia, and GFAP in astrocytes from sensitized animals exposed to the pungent odor.

Conclusion:
Our findings demonstrate that nVNS inhibits mechanical nociception and represses expression of proteins associated with peripheral and central sensitization of trigeminal neurons in a novel rodent model of episodic migraine.
In Trauma Patients, the Occurrence of Early-Onset Nosocomial Infections is Associated With Increased Plasma Concentrations of Chromogranin A
Authors: Schneider Francis , Marban Céline , Ajob Gharib , Helle Sophie , Guillot Max , Launoy Anne , Maestraggi Quentin , Scavello Francesco , Rohr Olivier , Metz-Boutigue Marie-Hélène 
Published: October 2017

ABSTRACT
In previously healthy persons suffering from acute illnesses, nosocomial infections (NIs) are frequent. Their prevalence suggests the existence of as yet unknown conditions that may promote care-related infection. This study assessed whether the measurement of plasma chromogranin A, a stress-related protein involved in innate defense, is related to NI risk, and whether any chromogranin A-derived fragment included in vasostatin-I displays immunosuppressive activities related to AP-1 or NF-kappa B downregulation. At the clinical level, trauma patients and healthy controls were recruited to be eligible. Clinical histories were recorded, and standard biological tests (including plasma chromogranin A) were performed. For 9 randomly chosen patients and 16 controls, the time-dependent concentrations of chromogranin A (CGA) were assessed twice a day over 66 h. The data show that trauma patients present a higher value of CGA concentration during 66 h in comparison with healthy controls. In addition, patients maintaining this significant increase in CGA readily develop NIs. We therefore studied the effects of chromogranin A-derived peptides on monocytes, focusing on transcription factors that play a central role in inflammation. In vitro assay demonstrated that a chromogranin A-derived fragment (CGA47–70) displays a significant inhibition of NF-kappa B and AP-1 transcriptional activities in these cells. In conclusion, the occurrence of NI in trauma patients is associated with significantly increased plasma CGA concentrations. Downregulation of the two transcription factors by CGA47–70 might induce early acquired immune defect after a serious medical stress.
Graph Theoretical Analysis of Genome-Scale Data: Examination of Gene Activation Occurring in the Setting of Community-Acquired Pneumonia
Authors: Peck Palmer Octavia M. , Rogers Gary , Yende Sachin , Angus Derek C. , Clermont Gilles , Langston Michael A. 
Published: October 2017

ABSTRACT

Introduction:
We have previously reported evidence that Black individuals appear to have a significantly higher incidence of infection-related hospitalizations compared with White individuals. It is possible that the host immune response is responsible for this vital difference. In support of such a hypothesis, the aim of this study was to determine whether Black and White individuals exhibit differential whole blood gene network activation.

Methods:
We examined whole blood network activation in a subset of patients (n = 22 pairs, propensity score matched (1:1) Black and White patients) with community-acquired pneumonia (CAP) from the Genetic and Inflammatory Markers of Sepsis study. We employed day one whole blood transcriptomic data generated from this cohort and constructed co-expression graphs for each racial group. Pearson correlation coefficients were used to weight edges. Spectral thresholding was applied to ascribe significance. Innovative graph theoretical methods were then invoked to detect densely connected gene networks and provide differential structural analysis.

Results:
Propensity matching was employed to reduce potential bias due to confounding variables. Although Black and White patients had similar socio- and clinical demographics, we identified novel differences in molecular network activation—dense subgraphs known as paracliques that displayed complete gene connection for both White (three paracliques) and Black patients (one paraclique). Specifically, the genes that comprised the paracliques in the White patients include circadian loop, cell adhesion, mobility, proliferation, tumor suppression, NFκB, and chemokine signaling. However, the genes that comprised the paracliques in the Black patients include DNA and messenger RNA processes, and apoptosis signaling. We investigated the distribution of Black paracliques across White paracliques. Black patients had five paracliques (with almost complete connection) comprised of genes that are critical for host immune response widely distributed across 22 parcliques in the White population. Anchoring the analysis on two critical inflammatory mediators, interleukin (IL)-6 and IL-10 identified further differential network activation among the White and Black patient populations.

Conclusions:
These results demonstrate that, at the molecular level, Black and White individuals may experience different activation patterns with CAP. Further validation of the gene networks we have identified may help pinpoint genetic factors that increase host susceptibility to community-acquired pneumonia, and may lay the groundwork for personalized management of CAP.
Early Identification of the Patient with Endotheliopathy of Trauma by Arrival Serum Albumin
Authors: Gonzalez Rodriguez Erika , Cardenas Jessica C. , Lopez Ernesto , Cotton Bryan A. , Tomasek Jeffrey S. , Ostrowski Sisse R. , Baer Lisa A. , Stensballe Jakob , Holcomb John B. , Johansson Pär I. , Wade Charles E. 
Published: October 2017

ABSTRACT

Objective:
Traumatic endotheliopathy (EoT) is associated with glycocalyx breakdown and capillary leak resulting in the extravasation of proteins. We hypothesized that lower serum albumin levels are associated with EoT, poor outcomes, and can be used for early EoT screening in trauma patients.

Methods:
We enrolled severely injured trauma patients with serum albumin levels available on admission. Syndecan-1 and soluble thrombomodulin were quantified from plasma by ELISA. Demographic and clinical data were obtained. We evaluated the association of serum albumin and EoT+ (syndecan-1 level ≥40 ng/mL), followed by dichotomization by serum albumin level, and subgroup comparisons.

Results:
Of the 258 patients enrolled 92 (36.0%) were EoT+ (syndecan-1 ≥ 40 ng/mL). Median albumin levels in the EoT+ group were 3.4 g/dL, and 3.8 g/dL in EoT− patients, P < 0.05. In a multifactorial analysis, lower albumin levels were inversely associated with the likelihood of EoT+. With receiver characteristic curve analysis, we determined a cutoff albumin level < 3.6 g/dL for EoT+ prediction (area under the curve 0.70; 95% CI: 0.63, 0.77). After dichotomizing by albumin <3.6 or ≥3.6 g/dL, 51.5% of patients had low albumin. Low albumin patients were more likely to have EoT+, as well as higher soluble thrombomodulin (both P < 0.05). Furthermore, they required more frequently blood transfusions, had fewer hospital-free days and higher mortality rate than those with normal albumin.

Conclusions:
EoT is a syndrome associated with leakage of albumin from the intravascular compartment, which re-emphasizes that arrival albumin may be a novel and timely approach to the identification of patients needing endothelial rescue therapy.
Automated Reactive Accelerated Aging for Rapid In Vitro Evaluation of Neural Implants Performance
Authors: Street Matthew G. , Welle Cristin G. , Takmakov Pavel A. 
Published: October 2017

AbstractObjectiveNovel therapeutic applications for neural implants require miniaturized devices. Pilot clinical studies suggest that rapid failure of the miniaturized neural implants in the body presents a major challenge for this type of technology. Miniaturization imposes stricter requirements for reliability of materials and designs. Evaluation of neural implant performance over clinically relevant timescales presents time-and cost-prohibitive challenges for animal models.ApproachIn vitro reactive accelerated aging (RAA) was developed to expedite durability testing of these devices. RAA simulates an aggressive physiological environment associated with an immune response and implicated in device failure. It uses hydrogen peroxide, which mimics reactive oxygen species (ROS), and high temperature to accelerate chemical reactions that lead to device degradation. RAA accurately simulates the degradation pattern of neural implants observed in vivo, but requires daily maintenance and is prone to variability in performance.Main resultsThis work introduces automated reactive accelerated aging (aRAA) that is compatible with multiplexing. The core of aRAA is electrochemical detection for feedback control of hydrogen peroxide concentration, implemented with simple off-the shelf components.SignificanceaRAA allows multiple parallel experiments for a high-throughput optimization of reactive aging conditions to more quickly and more rigorously simulate the in vivo environment. aRAA is a cost-effective tool for rapid in vitro evaluation of durability of neural implants, ultimately expediting the development of a new generation of miniaturized devices with long functional lifespans.
Germline Contamination and Leakage in Whole Genome Somatic Single Nucleotide Variant Detection
Authors: Sendorek Dorota H. , Caloian Cristian , Ellrott Kyle , Bare J. Christopher , Yamaguchi Takafumi N. , Ewing Adam D. , Houlahan Kathleen E. , Norman Thea C. , Margolin Adam A. , Stuart Joshua M. , Boutros Paul C. 
Published: October 2017

AbstractBackgroundThe clinical sequencing of cancer genomes to personalize therapy is becoming routine across the world. However, concerns over patient re-identification from these data lead to questions about how tightly access should be controlled. It is not thought to be possible to re-identify patients from somatic variant data. However, somatic variant detection pipelines can mistakenly identify germline variants as somatic ones, a process called “germline leakage”. The rate of germline leakage across different somatic variant detection pipelines is not well-understood, and it is uncertain whether or not somatic variant calls should be considered re-identifiable. To fill this gap, we quantified germline leakage across 259 sets of whole-genome somatic single nucleotide variant (SNVs) predictions made by 21 teams as part of the ICGC-TCGA DREAM Somatic Mutation Calling Challenge.ResultsThe median somatic SNV prediction set contained 4,325 somatic SNVs and leaked one germline polymorphism. The level of germline leakage was inversely correlated with somatic SNV prediction accuracy and positively correlated with the amount of infiltrating normal cells. The specific germline variants leaked differed by tumour and algorithm. To aid in quantitation and correction of leakage, we created a tool, called GermlineFilter, for use in public-facing somatic SNV databases.ConclusionsThe potential for patient re-identification from leaked germline variants in somatic SNV predictions has led to divergent open data access policies, based on different assessments of the risks. Indeed, a single, well-publicized re-identification event could reshape public perceptions of the values of genomic data sharing. We find that modern somatic SNV prediction pipelines have low germline-leakage rates, which can be further reduced, especially for cloud-sharing, using pre-filtering software.
A simple, consistent estimator of heritability for genome-wide association studies
Authors: Schwartzman Armin , Schork Andrew J. , Zablocki Rong , Thompson Wesley K. 
Published: October 2017

AbstractAnalysis of genome-wide association studies (GWAS) is characterized by a large number of univariate regressions where an outcome, a quantitative trait, is regressed on hundreds of thousands to millions of genomic markers, i.e. single-nucleotide polymorphism (SNP) counts, one marker at a time. Assuming a linear model linking the markers to the outcome, this article proposes an estimator of the heritability of the trait, defined here as the fraction of the variance of the trait explained by the genomic markers in the study. The estimator, called GWAS heritability (GWASH) estimator, is easy to compute, highly interpretable, and is consistent as the number of markers and the sample size increase. More importantly, it can be computed from summary statistics typically reported in GWAS, not requiring access to the original data. The estimator takes full account of the linkage disequilibrium (LD) or correlation between the SNPs in the study through moments of the LD matrix, estimable from auxiliary datasets. Unlike other proposed estimators in the literature, the precision of the estimate is obtainable analytically, allowing for power and sample size calculations for heritability estimates.
Competitive inter-species interactions underlie the increased antimicrobial tolerance in multispecies brewery biofilms
Authors: Parijs Ilse , Steenackers Hans P. 
Published: October 2017

ABSTRACTGenetic diversity often enhances the tolerance of microbial communities against antimicrobial treatment. However the sociobiology underlying this antimicrobial tolerance remains largely unexplored. Here we analyze how inter-species interactions can increase antimicrobial tolerance. We apply our approach to 17 industrially-relevant multispecies biofilm models, based on species isolated from 58 contaminating biofilms in three breweries. Sulfathiazole is used as antimicrobial agent because it shows the highest activity out of 22 biofilm inhibitors tested. Our analysis reveals that competitive interactions dominate among species within brewery biofilms. We show that antimicrobial treatment can reduce the level of competition and therefore cause a subset of species to bloom. The result is a lower percentage inhibition of these species and increased tolerance. In addition, we show that the presence of competing species can also directly enhance the inherent tolerance of microbes to antimicrobial treatment, either because species protect each other or because they induce specific tolerance phenotypes as a response to competitors (i.e. competition sensing). Overall, our study emphasizes that the dominance of competitive interactions is central to the enhanced antimicrobial tolerance of the multispecies biofilms and that the activity of antimicrobials against multispecies biofilms cannot be predicted based on their effect against mono-cultures.
Strong binding activity of few transcription factors is a major determinant of open chromatin
Authors: Wei Bei , Jolma Arttu , Sahu Biswajyoti , Orre Lukas M. , Zhong Fan , Zhu Fangjie , Kivioja Teemu , Sur Inderpreet Kaur , Lehtiö Janne , Taipale Minna , Taipale Jussi 
Published: October 2017

AbstractIt is well established that transcription factors (TFs) play crucial roles in determining cell identity, and that a large fraction of all TFs are expressed in most cell types. In order to globally characterize activities of TFs in cells, we have developed a novel massively parallel protein activity assay, Active TF Identification (ATI) that measures DNA-binding activity of all TFs from any species or tissue type. In contrast to previous studies based on mRNA expression or protein abundance, we found that a set of TFs binding to only around ten distinct motifs display strong DNA-binding activity in any given cell or tissue type. Mass spectrometric identification of TFs revealed that within these highly active TFs, there were both housekeeping TFs, which were universally found in all cell types, and specific TFs, which were highly enriched in known factors that determine the fate of the analyzed tissue or cell type. The importance of a small subset of TFs for determining the overall accessible chromatin landscape of a cell suggests that gene regulatory logic may be simpler than what has previously been appreciated.
Health commodified, health communified: navigating digital consumptionscapes of well-being
Authors: Cavusoglu Lena , Demirbag-Kaplan Melike 
Published: October 2017

PurposeHistorically, research on perceptions of health either converged upon the meanings created and proposed by specialists in the healthcare industry or focused on people who have medical conditions. This approach has failed to capture how the meanings and notions of health have been evolving as medicine extends into non-medical spheres and has left gaps in the exploration of how the meanings surrounding health and well-being are constructed, negotiated and reproduced in lay discourse. This paper aims to fill this gap in the understanding of the perceptions surrounding health by investigating consumers’ digitized visual accounts on social media.Design/methodology/approachTextual network and visual content analyses of posts extracted from Instagram are used to derive conclusions on definitions of health and well-being as perceived by healthy lay individuals.FindingsResearch demonstrates that digital discourse of health is clustered around four F’s, namely, food, fitness, fashion and feelings, which can be categorized with respect to their degrees of representation on a commodification/communification versus bodily/spiritual well-being map.Originality/valueOur knowledge about the meanings of health as constructed and reflected by healthy lay people is very limited and even more so about how these meaning-making processes is realized through digital media. This paper contributes to theory by integrating consumers’ meaning-making literature into health perceptions, as well as investigating the role of social networks in enabling a consumptionscape of well-being. Besides a methodological contribution of using social network analysis on textual data, this paper also provides valuable insights for policy-makers, communicators and professionals of health.
Consumer imagination in marketing: a theoretical framework
Authors: Philips Barbara J. 
Published: October 2017

Purpose
Imagination is a complex mental process where consciousness departs from reality to create new content not currently found in existence. Imagination is key to marketing persuasion, but studies that examine consumer imagination in response to marketing messages illustrate confusing and sometimes contradictory perspectives about consumers’ mental processing. This paper aims to provide a review of the existing literature on consumer imagination relevant to marketing scholarship, and builds a new theoretical framework to organize and explain these papers.

Design/methodology/approach
A systematic review of the marketing literature was undertaken to identify all papers related to consumer imagination and its role in marketing persuasion. A focus was placed on empirical papers, review papers and meta-analyses.

Findings
A new conceptual framework was created to classify the consumer imagination literature based on both the characteristics and the content of imagination. The existing marketing literature was then organized into the framework. The framework helps to explain seeming contradictions between different studies as well as helps to collect similar studies together to summarize schools of thought.

Originality/value
The imagination framework presents an entirely new way of conceptualizing imagination research in marketing. This new categorization structure not only clarifies consumers’ use of imagination in response to marketing messages but also identifies questions for future research in this area of marketing theory.