bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between Tumor Vascularity, Vascular Endothelial Growth Factor Expression and PET/MRI Radiomic Signatures in Primary Clear-Cell–Renal-Cell-Carcinoma: Proof-of-Concept Study
Authors: Yin Qingbo , Hung Sheng-Che , Wang Li , Lin Weili , Fielding Julia R. , Rathmell W. Kimryn , Khandani Amir H. , Woods Michael E. , Milowsky Matthew I. , Brooks Samira A. , Wallen Eric. M. , Shen Dinggang 
Published: March 2017

AbstractStudies have shown that tumor angiogenesis is an essential process for tumor growth, proliferation and metastasis. Also, tumor angiogenesis is an important prognostic factor of clear cell renal cell carcinoma (ccRCC), as well as a factor in guiding treatment with antiangiogenic agents. Here, we attempted to find the associations between tumor angiogenesis and radiomic imaging features from PET/MRI. Specifically, sparse canonical correlation analysis was conducted on 3 feature datasets (i.e., radiomic imaging features, tumor microvascular density (MVD), and vascular endothelial growth factor (VEGF) expression) from 9 patients with primary ccRCC. In order to overcome the potential bias of intratumoral heterogeneity of angiogenesis, this study investigated the relationship between regional expressions of angiogenesis and VEGF, and localized radiomic features from different parts within the tumors. Our study highlighted the significant strong correlations between radiomic features and MVD, and also demonstrated that the spatiotemporal features extracted from DCE-MRI provided stronger radiomic correlation to MVD than the textural features extracted from Dixon sequences and FDG PET. Furthermore, PET/MRI, which takes advantage of the combined functional and structural information, had higher radiomics correlation to MVD than solely utilizing PET or MRI alone.
Circulating TNF Receptors 1 and 2 Predict Mortality in Patients with End-stage Renal Disease Undergoing Dialysis
Authors: Gohda Tomohito , Maruyama Shuntaro , Kamei Nozomu , Yamaguchi Saori , Shibata Terumi , Murakoshi Maki , Horikoshi Satoshi , Tomino Yasuhiko , Ohsawa Isao , Gotoh Hiromichi , Nojiri Shuko , Suzuki Yusuke 
Published: March 2017

AbstractRelatively high circulating levels of soluble tumor necrosis factor (TNF) receptors (TNFRs: TNFR1, TNFR2) have been associated with not only progression to end-stage renal disease but also mortality in patients with diabetes. It remains unknown whether elevated TNFR levels in haemodialysis patients are associated with mortality. We studied 319 patients receiving maintenance haemodialysis who were followed for a median of 53 months. Circulating markers of TNF pathway (TNFα and TNFRs) were measured with immunoassay. Strong positive correlations between TNFR1 and TNFR2 were observed (r = 0.81, P < 0.0001). During follow-up, 88 (27.6%) patients died of any cause (40 [45.5%] died of cardiovascular disease). In the Cox multivariate model, either TNFR but not TNFα remained a significant independent predictor of all-cause mortality (TNFR1: hazard ratio [HR] 2.34, 95% confidence interval [CI], 1.50–3.64; TNFR2: HR 2.13, 95% CI 1.38–3.29) after adjustment for age, prior cardiovascular disease, predialysis systolic blood pressure, and large systolic blood pressure decline during dialysis session. For cardiovascular mortality, significance was only observed in TNFR1 (TNFR1: HR 2.15, 95% CI 1.13–4.10). Elevated TNFRs levels were associated with the risk of cardiovascular and/or all-cause mortality independent of all relevant covariates in patients undergoing haemodialysis.
Nucleophosmin Interacts with PIN2/TERF1-interacting Telomerase Inhibitor 1 (PinX1) and Attenuates the PinX1 Inhibition on Telomerase Activity
Authors: Cheung Derek Hang-Cheong , Ho Sai-Tim , Lau Kwok-Fai , Jin Rui , Wang Ya-Nan , Kung Hsiang-Fu , Huang Jun-Jian , Shaw Pang-Chui 
Published: March 2017

AbstractTelomerase activation and telomere maintenance are critical for cellular immortalization and transformation. PIN2/TERF1-interacting telomerase inhibitor 1 (PinX1) is a telomerase regulator and the aberrant expression of PinX1 causes telomere shortening. Identifying PinX1-interacting proteins is important for understanding telomere maintenance. We found that PinX1 directly interacts with nucleophosmin (NPM), a protein that has been shown to positively correlate with telomerase activity. We further showed that PinX1 acts as a linker in the association between NPM and hTERT, the catalytic subunit of telomerase. Additionally, the recruitment of NPM by PinX1 to the telomerase complex could partially attenuate the PinX1-mediated inhibition on telomerase activity. Taken together, our data reveal a novel mechanism that regulates telomerase activation through the interaction between NPM, PinX1 and the telomerase complex.
A Peptide Amphiphile Organogelator of Polar Organic Solvents
Authors: Rouse Charlotte K. , Martin Adam D. , Easton Christopher J. , Thordarson Pall 
Published: March 2017

AbstractA peptide amphiphile is reported, that gelates a range of polar organic solvents including acetonitrile/water, N,N-dimethylformamide and acetone, in a process dictated by β-sheet interactions and facilitated by the presence of an alkyl chain. Similarities with previously reported peptide amphiphile hydrogelators indicate analogous underlying mechanisms of gelation and structure-property relationships, suggesting that peptide amphiphile organogel design may be predictably based on hydrogel precedents.
Mapping sites of herpes simplex virus type 1 glycoprotein D that permit insertions and impact gD and gB receptors usage
Authors: Fan Qing , Kopp Sarah , Connolly Sarah A. , Muller William J. , Longnecker Richard 
Published: March 2017

AbstractGlycoprotein D (gD) of herpes simplex virus type 1 (HSV-1) is one of four glycoproteins essential for HSV entry and cell fusion. The purpose of this study was to determine the plasticity of gD to tolerate insertion or deletion mutations and to construct an oncolytic HSV-1 that utilizes the disialoganglioside GD2 as a HSV-1 entry receptor. We found that the N-terminus of gD tolerates long insertions, whereas residues adjacent to the gD Ig-like V-type core tolerated shorter insertions (up to 15 amino acids), but not greater than 60 amino acids. Recombinant HSV-1 containing the ch14.18 single chain variable fragment (scFv) at the N-terminus of gD failed to mediate entry, even though the ch14.18 scFv-gD chimera Fc bound to neuroblastoma cells expressing GD2. Finally, we found that hyperfusogenic gB mutants enhanced fusion to a greater degree with the gB receptor the paired immunoglobulin-like type 2 receptor alpha (PILRα) than with gD receptors HVEM and nectin-1. Hyperfusogenic gB could restore the fusion function with PILRα when a gD constructed contained only the “profusion domain” (PFD), suggesting the hyperfusogenic form of gB may regulate fusion of PILRα via a novel mechanism through gH/gL and the gD PFD.
Time-lapse imaging reveals highly dynamic structural maturation of postnatally born dentate granule cells in organotypic entorhino-hippocampal slice cultures
Authors: Radic Tijana , Jungenitz Tassilo , Singer Mathias , Beining Marcel , Cuntz Hermann , Vlachos Andreas , Deller Thomas , Schwarzacher Stephan W. 
Published: March 2017

AbstractNeurogenesis of hippocampal granule cells (GCs) persists throughout mammalian life and is important for learning and memory. How newborn GCs differentiate and mature into an existing circuit during this time period is not yet fully understood. We established a method to visualize postnatally generated GCs in organotypic entorhino-hippocampal slice cultures (OTCs) using retroviral (RV) GFP-labeling and performed time-lapse imaging to study their morphological development in vitro. Using anterograde tracing we could, furthermore, demonstrate that the postnatally generated GCs in OTCs, similar to adult born GCs, grow into an existing entorhino-dentate circuitry. RV-labeled GCs were identified and individual cells were followed for up to four weeks post injection. Postnatally born GCs exhibited highly dynamic structural changes, including dendritic growth spurts but also retraction of dendrites and phases of dendritic stabilization. In contrast, older, presumably prenatally born GCs labeled with an adeno-associated virus (AAV), were far less dynamic. We propose that the high degree of structural flexibility seen in our preparations is necessary for the integration of newborn granule cells into an already existing neuronal circuit of the dentate gyrus in which they have to compete for entorhinal input with cells generated and integrated earlier.
Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding
Authors: Huang Renjie , Bonnichon Arnaud , Claridge Timothy D. W. , Leung Ivanhoe K. H. 
Published: March 2017

AbstractWaterLOGSY is a popular ligand-observed NMR technique to screen for protein-ligand interactions, yet when applied to measure dissociation constants (KD) through ligand titration, the results were found to be strongly dependent on sample conditions. Herein, we show that accurate KDs can be obtained by waterLOGSY with optimised experimental setup.
Copper-catalyzed Direct 2-Arylation of Benzoxazoles and Benzoimidazoles with Aryl Bromides and Cytotoxicity of Products
Authors: Jia Nan-Nan , Tian Xin-Chuan , Qu Xiao-Xia , Chen Xing-Xiu , Cao Ya-Nan , Yao Yun-Xin , Gao Feng , Zhou Xian-Li 
Published: March 2017

AbstractAn efficient copper-catalyzed direct 2-arylation of benzoxazoles and benzoimidazoles with aryl bromides is presented. The CuI/PPh3-based catalyst promotes the installation of various aryl and heteroaryl groups through a C-H activation process in good to excellent yields. The cytotoxicity of obtained 2-aryl benzoxazoles (benzoimidazoles) was also evaluated and 1-methyl-2-(naphthalen-1-yl)benzoimidazole showed potential cytotoxicity.
Coexistence of spin ordering on ladders and spin dimer formation in a new-structure-type compound Sr2Co3S2O3
Authors: Lai Kwing To , Valldor Martin 
Published: March 2017

AbstractWe report on the syntheses and characterizations of single crystalline and polycrystalline Sr2Co3S2O3with a novel crystal structure type. It contains Co–O 2-leg rectangular ladders and necklace ladders. The two ladders share common legs and construct ahybrid spin ladder. A rare meridional heteroleptic octahedral coordination is found for the Co2+ions in the 2-leg ladder. Within the necklace ladders, the Co2+ions are intrans-octahedral coordination. An antiferromagnetic order is observed at TN ~ 267 K, while a broad maximum in magnetic susceptibility is found below TN. This relatively high ordering temperature among Co-based ladder compounds is related to the highly anisotropicmer-coordination of the Co2+ions. Thetrans-octahedrally coordinated Co2+ions, on the other hand, corresponds to the possible short-range magnetic correlations through dimers with an effective "Equation missing". This results in a rare situation that spin ordering and spin dimers coexist down to 2 K.
Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity
Authors: Fu Shi , Xu Huan , Gu Meng , Liu Chong , Wang Qiong , Wan Xiang , Chen Yanbo , Chen Qi , Peng Yubing , Cai Zhikang , Zhou Juan , Wang Zhong 
Published: March 2017

AbstractThe incidence of benign prostatic hyperplasia (BPH) is increasing among obese individuals, but few studies have fully explained the underlying mechanisms. We aimed to elucidate the relationship between obesity and BPH. Herein, we show that in prostatic epithelial and stromal cells, adiponectin exerts multifunctional effects including anti-proliferation, blocking of G1/S-phase progression and the promotion of apoptosis via inhibiting the MEK-ERK-p90RSK axis. Furthermore, we found that a high-fat diet (HFD) led to adiponectin deficiency and microscopic BPH in a mouse model of obesity. And an adiponectin supplement protected the obese mice from microscopic BPH. The present study provides evidence that adiponectin is a protective regulator in the development and progression of BPH and that adiponectin deficiency causally links BPH with obesity.
Ebola virus infection kinetics in chimeric mice reveal a key role of T cells as barriers for virus dissemination
Authors: Lüdtke Anja , Ruibal Paula , Wozniak David M. , Pallasch Elisa , Wurr Stephanie , Bockholt Sabrina , Gómez-Medina Sergio , Qiu Xiangguo , Kobinger Gary P. , Rodríguez Estefanía , Günther Stephan , Krasemann Susanne , Idoyaga Juliana , Oestereich Lisa , Muñoz-Fontela César 
Published: March 2017

AbstractEbola virus (EBOV) causes severe systemic disease in humans and non-human primates characterized by high levels of viremia and virus titers in peripheral organs. The natural portals of virus entry are the mucosal surfaces and the skin where macrophages and dendritic cells (DCs) are primary EBOV targets. Due to the migratory properties of DCs, EBOV infection of these cells has been proposed as a necessary step for virus dissemination via draining lymph nodes and blood. Here we utilize chimeric mice with competent hematopoietic-driven immunity, to show that EBOV primarily infects CD11b+ DCs in non-lymphoid and lymphoid tissues, but spares the main cross-presenting CD103+ DC subset. Furthermore, depletion of CD8 and CD4 T cells resulted in loss of early control of virus replication, viremia and fatal Ebola virus disease (EVD). Thus, our findings point out at T cell function as a key determinant of EVD progress and outcome.
Elevated Fibroblast growth factor 21 (FGF21) in obese, insulin resistant states is normalised by the synthetic retinoid Fenretinide in mice
Authors: Morrice Nicola , Mcilroy George D. , Tammireddy Seshu R. , Reekie Jennifer , Shearer Kirsty D. , Doherty Mary K. , Delibegović Mirela , Whitfield Phillip D. , Mody Nimesh 
Published: March 2017

AbstractFibroblast growth factor 21 (FGF21) has emerged as an important beneficial regulator of glucose and lipid homeostasis but its levels are also abnormally increased in insulin-resistant states in rodents and humans. The synthetic retinoid Fenretinide inhibits obesity and improves glucose homeostasis in mice and has pleotropic effects on cellular pathways. To identify Fenretinide target genes, we performed unbiased RNA-seq analysis in liver from mice fed high-fat diet ± Fenretinide. Strikingly, Fgf21 was the most downregulated hepatic gene. Fenretinide normalised elevated levels of FGF21 in both high-fat diet-induced obese mice and in genetically obese-diabetic Leprdbmice. Moreover, Fenretinide-mediated suppression of FGF21 was independent of body weight loss or improved hepatic insulin sensitivity and importantly does not induce unhealthy metabolic complications. In mice which have substantially decreased endogenous retinoic acid biosynthesis, Fgf21 expression was increased, whereas acute pharmacological retinoid treatment decreased FGF21 levels. The repression of FGF21 levels by Fenretinide occurs by reduced binding of RARα and Pol-II at the Fgf21 promoter. We therefore establish Fgf21 as a novel gene target of Fenretinide signalling via a retinoid-dependent mechanism. These results may be of nutritional and therapeutic importance for the treatment of obesity and type-2 diabetes.
Aberrant Epicardial Adipose Tissue Extracellular Matrix Remodeling in Patients with Severe Ischemic Cardiomyopathy: Insight from Comparative Quantitative Proteomics
Authors: Jiang Ding-Sheng , Zeng Hao-Long , Li Rui , Huo Bo , Su Yun-Shu , Fang Jing , Yang Qing , Liu Li-Gang , Hu Min , Cheng Cai , Zhu Xue-Hai , Yi Xin , Wei Xiang 
Published: March 2017

AbstractThere is ample evidence indicating that epicardial adipose tissue (EAT) volume and thickness is positively associated with coronary artery disease (CAD). However, the exact pathological changes in the human EAT after myocardial ischemia remains largely unclear. In the current study, we applied a comparative quantitative proteomics to elucidate the altered biological processes in the EAT of ischemic cardiomyopathy (ICM) patients. A total of 1649 proteins were successfully quantified in our study, among which 165 proteins were significantly changed (ratio <0.8 or >1.2 fold and p < 0.05 in both repetitions) in EAT of ICM individuals. Gene ontology (GO) enrichment analysis revealed that cardiac structure and cellular metabolism were over-represented among these regulated proteins. The hypertrophic cardiomyopathy, adrenergic signaling in cardiomyocytes, extracellular matrix (ECM)-receptor interaction, phagosome, Glycolysis/Gluconeogenesis, and PPAR signaling pathway were highlighted by the KEGG PATHWAY analysis. More importantly, we found that the proteins responsible for extracellular matrix organization were dramatically increased in EAT of ICM patients. In addition, the picrosirius red (PSR) staining results showed that the collagen fiber content was prominently increased, which indicated the EAT of ICM individuals underwent extracellular matrix remodeling and ERK1/2 activation maybe responsible for these pathological changes partially.
Passion fruit-like nano-architectures: a general synthesis route
Authors: Cassano D. , David J. , Luin S. , Voliani V. 
Published: March 2017

AbstractNoble metal nanostructures have demonstrated a number of intriguing features for both medicine and catalysis. However, accumulation issues have prevented their clinical translation, while their use in catalysis has shown serious efficiency and stability hurdles. Here we introduce a simple and robust synthetic protocol for passion fruit-like nano-architectures composed by a silica shell embedding polymeric arrays of ultrasmall noble metal nanoparticles. These nano-architectures show interesting features for both oncology and catalysis. They avoid the issue of persistence in organism thanks to their fast biodegradation in renal clearable building blocks. Furthermore, their calcination results in yolk-shell structures composed by naked metal or alloy nanospheres shielded from aggregation by a silica shell.
Strain-tuned enhancement of ferromagnetic TC to 176 K in Sm-doped BiMnO3 thin films and determination of magnetic phase diagram
Authors: Choi Eun-Mi , Kleibeuker Josée E. , MacManus-Driscoll Judith L. 
Published: March 2017

AbstractBiMnO3 is a promising multiferroic material but it’s ferromagnetic TC is well below room temperature and the magnetic phase diagram is unknown. In this work, the relationship between magnetic transition temperature (TC) and the substrate induced (pseudo-) tetragonal distortion (ratio of out-of-plane to in-plane lattice parameters, c/a) in BiMnO3 thin films, lightly doped to optimize lattice dimensions, was determined. For c/a > 0.99, hidden antiferromagnetism was revealed and the magnetisation versus temperature curves showed a tail behaviour, whereas for c/a < 0.99 clear ferromagnetism was observed. A peak TC of up to 176 K, more than 70 K higher than for bulk BiMnO3, was achieved through precise strain tuning. The TC was maximised for strong tensile in-plane strain which produced weak octahedral rotations in the out-of-plane direction, an orthorhombic-like structure, and strong ferromagnetic coupling.
LRP1 influences trafficking of N-type calcium channels via interaction with the auxiliary α2δ-1 subunit
Authors: Kadurin Ivan , Rothwell Simon W. , Lana Beatrice , Nieto-Rostro Manuela , Dolphin Annette C. 
Published: March 2017

AbstractVoltage-gated Ca2+ (CaV) channels consist of a pore-forming α1 subunit, which determines the main functional and pharmacological attributes of the channel. The CaV1 and CaV2 channels are associated with auxiliary β- and α2δ-subunits. The molecular mechanisms involved in α2δ subunit trafficking, and the effect of α2δ subunits on trafficking calcium channel complexes remain poorly understood. Here we show that α2δ-1 is a ligand for the Low Density Lipoprotein (LDL) Receptor-related Protein-1 (LRP1), a multifunctional receptor which mediates trafficking of cargoes. This interaction with LRP1 is direct, and is modulated by the LRP chaperone, Receptor-Associated Protein (RAP). LRP1 regulates α2δ binding to gabapentin, and influences calcium channel trafficking and function. Whereas LRP1 alone reduces α2δ-1 trafficking to the cell-surface, the LRP1/RAP combination enhances mature glycosylation, proteolytic processing and cell-surface expression of α2δ-1, and also increase plasma-membrane expression and function of CaV2.2 when co-expressed with α2δ-1. Furthermore RAP alone produced a small increase in cell-surface expression of CaV2.2, α2δ-1 and the associated calcium currents. It is likely to be interacting with an endogenous member of the LDL receptor family to have these effects. Our findings now provide a key insight and new tools to investigate the trafficking of calcium channel α2δ subunits.
Vlasouliolides A-D, four rare C17/C15 sesquiterpene lactone dimers with potential anti-inflammatory activity from Vladimiria souliei
Authors: Chen Li-Ping , Wu Guo-Zhen , Zhang Jian-Ping , Ye Ji , Liu Qing-Xin , Shen Yun-Heng , Li Hui-Liang , Zhang Wei-Dong 
Published: March 2017

AbstractVlasouliolides A-D (1–4), four rare sesquiterpene lactone dimers, were isolated from Vladimiria souliei. The common structural characteristic of 1–4 is the C32 skeleton comprising two sesquiterpene lactone units linked by a C11-C13′ single bond with one acetyl connected to the C-13 position of one of the two sesquiterpene lactone units. The stereochemistries of 1–4 were assigned by a combination of NOESY correlations and Cu-Κα X-ray crystallographic analyses. Compounds 1–4 strongly inhibited the production of NO in LPS-stimulated RAW 264.7 cells. Furthermore, 1 and 2 inhibited the activation of NF-κB in LPS-induced 293T cells.
Glycol Derived Carbon- TiO2 as Low Cost and High Performance Anode Material for Sodium-Ion Batteries
Authors: Tao Hongwei , Zhou Min , Wang Kangli , Cheng Shijie , Jiang Kai 
Published: March 2017

AbstractCarbon coated TiO2 (TiO2@C) is fabricated by a convenient and green one-pot solvothermal method, in which ethylene glycol serve as both the reaction medium and carbon source without the addition of any other carbon additives. During the solvothermal process, ethylene glycol polymerize and coordinate with Ti4+ to form the polymeric ligand precursor, then the polymer brushes carbonize and convert to homogeneous carbon layer firmly anchored on the TiO2 nanoparticles (~1 nm thickness). The polymerization and carbonization process of the ethylene glycol is confirmed by FT-IR, Raman, TG and TEM characterizations. Benefiting from the well-dispersed nanoparticles and uniform carbon coating, the as-prepared TiO2@C demonstrate a high reversible capacity of 317 mAh g−1 (94.6% of theoretical value), remarkable rate capability of 125 mAh g−1 at 3.2 A g−1 and superior cycling stability over 500 cycles, possibly being one of the highest capacities reported for TiO2.