bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of cadmium on kitl pre‐mRNA alternative splicing in murine ovarian granulosa cells and its associated regulation by miRNAs
Authors: Wang Wenxiang , Chen Jie , Luo Lingfeng , Li Yuchen , Liu Jin , Zhang Wenchang 
Published: September 2017

AbstractIn this study, we established an in vitro exposure model of murine ovarian granulosa cells to observe the effect of Cd on alternative splicing of the kitl pre‐mRNA and subsequently to explore the role of kitl gene expression regulation‐related miRNAs through miRNA prediction, miRNA chip, bioinformatics and real‐time quantitative polymerase chain reaction analyses. Our results showed that the kitl1/kitl2 mRNA ratio was significantly different (P < 0.05) at different dosages and times. The miRNA chip analysis showed that the miRNA expression profiles for the Cd treatment were significantly changed, and the expression of 29 miRNAs involved in alternative splicing of the kitl pre‐mRNA was changed. The gene ontology analysis showed that the target gene functions of these 29 miRNAs were mainly enriched in the biological processes of cell metabolism regulation, post‐transcriptional regulation of mRNA, interleukin‐6‐mediated signal transduction, cell cycle, cell proliferation, differentiation and migration. The pathway enrichment analysis showed that the target genes of the differentially expressed miRNAs were mainly enriched in the Ras signaling pathway, the Rap1 signaling pathway, the Foxo signaling pathway, the Hippo signaling pathway, the MAPK signaling pathway and the carcinogenic pathway. Polymerase chain reaction verification results showed that compared to the control group, the variation trends in the expression of mmu‐miR‐27a‐3p, mmu‐miR‐34b‐5p, mmu‐miR‐297a‐3p, mmu‐miR‐129‐5p and mmu‐miR‐107‐3p in the 4 hour 10 μm Cd treatment group were basically the same as that of the chip result. Our results indicate that Cd exposure can affect alternative splicing of the kitl pre‐mRNA in ovarian granulosa cells, and miRNAs play regulatory roles in the alternative splicing of kitl.
Development and characterization of electrosprayed nanoparticles for encapsulation of Curcumin
Authors: Esmaili Zahra , Bayrami Samaneh , Dorkoosh Farid Abedin , Akbari Javar Hamid , Seyedjafari Ehsan , Zargarian Seyed Shahrooz , Haddadi‐Asl Vahid 
Published: September 2017

AbstractCurcumin has been proven to be an effective herbal derived anti‐inflammatory and antioxidant biocompatible agent. In this research, poly(lactic‐co‐glycolic acid) (PLGA) (as a biocompatible and generally recognized as safe (GRAS) polymer) nanoparticles containing Curcumin were electrosprayed from different polymeric solutions with different concentrations for the first time. Morphology of these nanoparticles in the absence/presence of Curcumin was evaluated by scanning electron microscope, transmission electron microscope, and X‐ray photoelectron spectroscopy analyses. Perfectly shaped nanoparticles with an average size of 300 and 320 nm were observed for neat and Curcumin‐loaded PLGA, respectively. Curcumin‐loaded electrosprayed nanoparticles showed a normal moderate initial burst and then a prolonged release period. Weibull, Peppas, and modified Korsmeyer–Peppas models were applied to study the kinetic and mechanism of Curcumin release from PLGA nanoparticles. Results showed high specific surface area and spherical geometry of the nanoparticles. Effectiveness of the electrospray method as a promising technique for preparing Curcumin‐loaded nanoparticles was confirmed in this study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 285–292, 2018.
Nerve‐specific, xenogeneic extracellular matrix hydrogel promotes recovery following peripheral nerve injury
Authors: Prest Travis A. , Yeager Eric , LoPresti Samuel T. , Zygelyte Emilija , Martin Matthew J. , Dong Longying , Gibson Alexis , Olutoye Oluyinka O. , Brown Bryan N. , Cheetham Jonathan 
Published: September 2017

AbstractPeripheral nerve possesses the inherent ability to regrow and recover following injury. However, nerve regeneration is often slow and incomplete due to limitations associated with the local microenvironment during the repair process. Manipulation of the local microenvironment at the site of nerve repair, therefore, represents a significant opportunity for improvement in downstream outcomes. Macrophages and Schwann cells play a key role in the orchestration of early events after peripheral nerve injury. We describe the production, characterization, and use of an injectable, peripheral nerve‐specific extracellular matrix‐based hydrogel (PNSECM) for promoting modulation of the local macrophage and Schwann cell responses at the site of nerve repair in a rodent model of sciatic nerve injury. We show that PNSECM hydrogels largely maintain the matrix structure associated with normal native peripheral nerve tissue. PNSECM hydrogels were also found to promote increased macrophage invasion, higher percentages of M2 macrophages and enhanced Schwann cell migration when used as a lumen filler in a rodent model of nerve gap repair using an inert nerve guidance conduit. These results suggest that an injectable PNSECM hydrogel can provide a supportive, bioactive scaffold which promotes repair of peripheral nerve in vivo. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 450–459, 2018.
Possibility of quantitative T2‐mapping MRI of cartilage near metal in high tibial osteotomy: A human cadaver study
Authors: Verschueren Joost , Meuffels Duncan E. , Bron Esther E. , Klein Stefan , Kleinrensink Gert‐Jan , Verhaar Jan A. N. , Bierma‐Zeinstra Sita M. A. , Krestin Gabriel P. , Wielopolski Piotr A. , Reijman Max , Oei Edwin H. G. 
Published: September 2017

ABSTRACTT2‐mapping is a widely used quantitative MRI technique in osteoarthritis research. An important challenge for its application in the context of high tibial osteotomy (HTO) is the presence of metallic fixation devices. In this study, we evaluated the possibility of performing T2‐mapping after a HTO, by assessing the extent of magnetic susceptibility artifacts and the influence on T2 relaxation times caused by two commonly used fixation devices. T2‐mapping with a 3D fast spin‐echo sequence at three Tesla was performed on 11 human cadaveric knee joints before and after implantation of a titanium plate and screws (n = 5) or cobalt chrome staples (n = 6). Mean T2 relaxation times were calculated in six cartilage regions, located in the distal and posterior cartilage of femoral condyles and the cartilage of tibial plateaus, both medially and laterally. T2 relaxation times before and after the implantation were compared with paired t‐tests and Wilcoxon rank tests. Due to the extent of the magnetic susceptibility artifact, it was not possible to segment the knee cartilage and thus calculate T2 relaxation times in the lateral weight‐bearing femoral and tibial cartilage regions only in the cobalt chrome group. In all cartilage regions of the titanium implanted knees and those unaffected by artifacts due to cobalt chrome implants, T2 relaxation times did not significantly differ between the two scans. Our results suggest that accurate T2‐mapping after a HTO procedure is possible in all areas after implantation of a titanium fixation device and in most areas after implantation of a cobalt chrome fixation device. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1206–1212, 2018.
Blood–Brain Barrier Permeability of Green Tea Catechin Metabolites and their Neuritogenic Activity in Human Neuroblastoma SH‐SY5Y Cells
Authors: Unno Keiko , Pervin Monira , Nakagawa Aimi , Iguchi Kazuaki , Hara Aya , Takagaki Akiko , Nanjo Fumio , Minami Akira , Nakamura Yoriyuki 
Published: September 2017

ScopeTo understand the mechanism by which green tea lowers the risk of dementia, focus was placed on the metabolites of epigallocatechin gallate (EGCG), the most abundant catechin in green tea. Much of orally ingested EGCG is hydrolyzed to epigallocatechin (EGC) and gallic acid. In rats, EGC is then metabolized mainly to 5‐(3′,5′‐dihydroxyphenyl)‐γ‐valerolactone (EGC‐M5) and its conjugated forms, which are distributed to various tissues. Therefore, we examined the permeability of these metabolites into the blood–brain barrier (BBB) and nerve cell proliferation/differentiation in vitro.Methods and ResultsThe permeability of EGC‐M5, glucuronide, and the sulfate of EGC‐M5, pyrogallol, as well as its glucuronide into the BBB were examined using a BBB model kit. Each brain‐ and blood‐side sample was subjected to liquid chromatography tandem‐mass spectrometry analysis. BBB permeability (%, in 0.5 h) was 1.9–3.7%. In human neuroblastoma SH‐SY5Y cells, neurite length was significantly prolonged by EGC‐M5, and the number of neurites was increased significantly by all metabolites examined.ConclusionThe permeability of EGC‐M5 and its conjugated forms into the BBB suggests that they reached the brain parenchyma. In addition, the ability of EGC‐M5 to affect nerve cell proliferation and neuritogenesis suggests that EGC‐M5 may promote neurogenesis in the brain.
Psychosocial outcomes in adults with type 1 diabetes following a novel ‘short course’ structured flexible MDI therapy self‐management programme
Authors: Knight Brigid A , Hickman Ingrid J , Gibbons Kristen , Taylor Janet , McIntyre Harold D 
Published: September 2017

AbstractOur objectives were to evaluate the psychosocial impact of a novel education programme, comprising an abbreviated curriculum based on the ‘core elements’ of insulin self‐management education, with a view to identifying education options for those unable to attend a five‐day Dose Adjustment for Normal Eating (DAFNE) programme.The novel programme was developed by DAFNE course facilitators and trialled using participants naive to flexible multiple daily injection (MDI) education. Post‐course treatment satisfaction, wellbeing and diabetes distress were compared to baseline.Twenty‐one adults attended the programme and 16 participants returned three months post‐course. Significant improvements were seen in W‐BQ12 total score, PAID score and DTSQ, equivalent to those observed following participation in the five‐day programme.It was concluded that short course flexible MDI education is well tolerated by adults with type 1 diabetes, with improvements in treatment satisfaction and psychosocial outcome that are consistent with the five‐day DAFNE programme. Further investigation of the longer‐term impact on psychosocial and glycaemic outcomes of this novel curriculum is warranted. Copyright © 2017 John Wiley & Sons.
Advance to Derby: it's not about the journey, it's about the destination
Authors: King Paru 
Published: September 2017

AbstractThe 2017 Mary MacKinnon lecture described how we worked across organisational boundaries to improve diabetes care in Derby, putting the user at the centre of their care. These are principles that were important to Mary McKinnon. The Derby Model of integrated diabetes care resulted in improvements in all six domains of quality improvement, and saved £800 000 a year. Using preconception care and the PROCEED project as an example, the application of the Derby principles to a subspecialist area was considered. PROCEED also improved outcomes and saved £68 000 in its first year. Despite a challenging journey with the changes in commissioning, we are now spreading our integrated diabetes model to the whole of Southern Derbyshire. Copyright © 2017 John Wiley & Sons.
Fault estimation and prediction for nonlinear stochastic system with intermittent observations
Authors: Ding Bo , Fang Huajing 
Published: September 2017

SummaryThis paper is concerned with the fault estimation and prediction problems for a class of nonlinear stochastic systems with intermittent observations. Based on the extended Kalman filter and Kalman filter, the fault and state are simultaneously estimated, and then, it is extended to the case of intermittent observations. Meanwhile, the boundedness of the estimation error is also discussed. Once the fault is detected, the parameters of each fault are identified by the linear regression method. Then, the future fault signal can be predicted by the parameters of the fault. The effectiveness of the proposed algorithm is verified by the simulation of the 3‐tank system.
Oncological Safety and Technical Feasibility of Nipple‐Sparing Mastectomy for Breast Cancer: The Hong Kong Experience
Authors: Chan Yolanda Ho‐Yan , Yau Wai‐Ming , Cheung Polly Suk‐Yee 
Published: September 2017

AbstractBackgroundNipple‐sparing mastectomy (NSM) has gained widespread popularity in recent years. Nonetheless, patient selection, technical consideration and oncological safety of its extension to breast cancer treatment remain uncertain. Few publications have reviewed the application of NSM in Asian populations.MethodsWe retrospectively reviewed 91 women with malignant breast tumours, who underwent 97 NSM in Hong Kong Sanatorium and Hospital from 2009 to 2015. Breast cancer patients who required mastectomy and opted for immediate reconstruction were considered for NSM if they showed no obvious nipple involvement clinically. All breast specimens were subjected to intraoperative pathological examination of the retroareolar tissue to exclude occult tumour infiltration before the final decision of nipple–areola complex (NAC) preservation. Clinical parameters, tumour characteristics and oncological outcomes were analyzed.ResultsCarcinoma of the breast accounts for 99.0% of our indications for therapeutic NSM. Almost all NSM were accompanied with immediate reconstruction. Abnormal pathology was shown in retroareolar tissue of ten patients (10.3%), and seven of these NAC were excised due to tumour involvement detected by intraoperative frozen section. Six (6.2%) NSM were complicated with superficial epidermolysis. Yet, there was no delayed NAC excision because of nipple necrosis. Overall NAC preservation rate reached 92.8%. Local and/or distant recurrences occurred in four patients (4.1%) after a mean follow‐up of 20.6 months. One NAC recurrence was documented.ConclusionOur series support the oncological safety of NSM after exclusion of neoplastic NAC involvement preliminarily by intraoperative frozen section and definitively by final pathology. Its technical feasibility is well proven by the low nipple necrosis rate.
Survival in idursulfase‐treated and untreated patients with mucopolysaccharidosis type II: data from the Hunter Outcome Survey (HOS)
Authors: Burton Barbara K. , Jego Virginie , Mikl Jaromir , Jones Simon A. 
Published: September 2017

AbstractMucopolysaccharidosis type II (MPS II; Hunter syndrome; OMIM 309900) is a life‐limiting, multisystemic disease with varying presentation and severity. Enzyme replacement therapy with intravenous idursulfase (EC 3.1.6.13) has been available since 2006. Data from the Hunter Outcome Survey (July 2016) were used to compare survival in idursulfase‐treated (n = 800) and untreated (n = 95) male patients followed prospectively in this multinational, observational registry. Median age at symptom onset was similar for the treated and untreated groups (1.6 and 1.5 years, respectively), as was median age at diagnosis (3.3 and 3.2 years) and the proportion of patients with cognitive impairment (58.0%; 57.9%). The proportion of idursulfase‐treated patients differed according to geographical region. Overall, 124/800 (15.5%) treated and 28/95 (29.5%) untreated patients had died. Respiratory failure was the most common cause of death (treated, 43/124 [34.7%]; untreated, 10/28 [35.7%]). Median survival (95% confidence interval [CI]) was 33.0 (30.4, 38.4) years in treated patients and 21.2 (16.1, 31.5) years in untreated patients; median follow‐up time from birth to death or last visit was 13.0 and 15.1 years, respectively. A Cox model adjusted for treatment status, cognitive impairment, region and age at diagnosis indicated a 54% lower risk of death in treated compared with untreated patients: hazard ratio (HR), 0.46 (95% CI: 0.29, 0.72). Patients with cognitive impairment had nearly a fivefold higher risk of death than those without (HR, 4.84 [3.13, 7.47]). This analysis in a large population of patients with MPS II indicates for the first time that idursulfase treatment is associated with increased survival.
Revision Total Shoulder Arthroplasty is Associated with Increased Thirty-Day Postoperative Complications and Wound Infections Relative to Primary Total Shoulder Arthroplasty
Authors: Boddapati Venkat , Fu Michael C. , Schairer William W. , Gulotta Lawrence V. , Dines David M. , Dines Joshua S. 
Published: September 2017

Background With an increasing volume of primary total shoulder arthroplasties (TSA), the number of revision TSA cases is expected to increase as well. However, the postoperative medical morbidity of revision TSA has not been clearly described. Questions/Purposes The purpose of this study was to determine the rate of postoperative complications following revision TSA, relative to primary TSA. In addition, we sought to identify independent predictors of complications, as well as to compare operative time and postoperative length of stay between primary and revision TSA. Methods Patients who underwent primary/revision TSA between 2005 and 2015 were identified in the American College of Surgeons National Surgical Quality Improvement Program. Differences in complications, readmission rates, operative time, length of stay, and predictors of complications were evaluated using bivariate and multivariate analyses. Results A total of 10, 371 primary TSA (95.4%) and 496 revision TSA cases (4.6%) were identified. The overall complication rate was 6.5% in primary and 10.7% in revision TSA patients ( p < 0.001). Multivariate analysis identified an increased risk of any complication (odds ratio 1.73, p < 0.001), major complication (2.08, p = 0.001), and wound infection (3.45, p = 0.001) in revision TSA patients, relative to primary cases. Operative time was increased in revision cases (mean ± standard deviation, 125 ± 62.5), relative to primary (115 ± 47.7, p < 0.001). Age > 75, female sex, history of diabetes or chronic obstructive pulmonary disease, and American Society of Anesthesiologists classification ≥ 3 were associated with increased risk of any complication. Smoking history was the only significant predictor of wound infection. Conclusion Revision TSA, in comparison to primary, poses an increased risk of postoperative complications, particularly wound infections. A history of smoking was an independent predictor of wound infections.
Polypeptide Profile, Amino Acid Composition and Some Functional Properties of Calabash Nutmeg (Monodora myristica) Flour and Protein Products
Authors: Akinyede Adedamola I. , Malomo Sunday A. , Fagbemi Tayo N. , Osundahunsi Oluwatooyin F. , Aluko Rotimi E. 
Published: September 2017

AbstractThe aim of this work was to compare the physicochemical and functional properties of calabash nutmeg (Monodora myristica) seed protein flour with those of protein‐enriched products (albumin, globulin, and protein isolate). Defatted M. myristica seed flour (MMF) was used to prepare various protein products. A NaCl extract of MMF was dialyzed against water to obtain the soluble albumin fraction (MMA) and a precipitated globulin fraction (MMG). MMF was also extracted with NaOH, the extract adjusted to pH 4.0 and the precipitated proteins collected as the isolate (MMI). Non‐reducing gel electrophoresis showed that the MMF, MMG and MMI had similar composition that was dominated by 55 and 110 kDa polypeptides while MMA consisted mainly of smaller (<35 kDa) polypeptides. However, under reducing conditions, the 110 kDa polypeptide was not observed. Amino acid composition revealed an Arg/Lys ratio that increased in the extracts (1.92, 2.28 and 2.11 for MMA, MMG and MMI, respectively) relative to that in MMF (1.85). MMA had 67.5–86.5% protein solubility in the pH 4.0–6.0 range while those of MMF, MMG, and MMI were 37.7–63.8, 2.7–69.4 and 3.8–55.1%, respectively. MMA, MMG and MMI were found to be better emulsifiers based on their smaller oil droplet sizes (8–14 μm) compared with the 14–33 μm for MMF emulsion. Maximum foaming capacity was highest for MMI (205%) when compared with MMA or MMG (150%) and MMF (89%). We conclude that protein enrichment led to significantly enhanced emulsion and foam‐forming properties but high solubility may have contributed to reduced emulsion stability.
The Growth, Scope, and Spatial Distribution of People With Felony Records in the United States, 1948–2010
Authors: Shannon Sarah K. S. , Uggen Christopher , Schnittker Jason , Thompson Melissa , Wakefield Sara , Massoglia Michael 
Published: September 2017

AbstractThe steep rise in U.S. criminal punishment in recent decades has spurred scholarship on the collateral consequences of imprisonment for individuals, families, and communities. Several excellent studies have estimated the number of people who have been incarcerated and the collateral consequences they face, but far less is known about the size and scope of the total U.S. population with felony convictions beyond prison walls, including those who serve their sentences on probation or in jail. This article develops state-level estimates based on demographic life tables and extends previous national estimates of the number of people with felony convictions to 2010. We estimate that 3 % of the total U.S. adult population and 15 % of the African American adult male population has ever been to prison; people with felony convictions account for 8 % of all adults and 33 % of the African American adult male population. We discuss the far-reaching consequences of the spatial concentration and immense growth of these groups since 1980.
Developing a novel risk prediction model for severe malarial anemia
Authors: Brickley E. B. , Kabyemela E. , Kurtis J. D. , Fried M. , Wood A. M. , Duffy P. E. 
Published: September 2017

AbstractAs a pilot study to investigate whether personalized medicine approaches could have value for the reduction of malaria-related mortality in young children, we evaluated questionnaire and biomarker data collected from the Mother Offspring Malaria Study Project birth cohort (Muheza, Tanzania, 2002–2006) at the time of delivery as potential prognostic markers for pediatric severe malarial anemia. Severe malarial anemia, defined here as aPlasmodium falciparuminfection accompanied by hemoglobin levels below 50 g/L, is a key manifestation of life-threatening malaria in high transmission regions. For this study sample, a prediction model incorporating cord blood levels of interleukin-1β provided the strongest discrimination of severe malarial anemia risk with a C-index of 0.77 (95% CI 0.70–0.84), whereas a pragmatic model based on sex, gravidity, transmission season at delivery, and bed net possession yielded a more modest C-index of 0.63 (95% CI 0.54–0.71). Although additional studies, ideally incorporating larger sample sizes and higher event per predictor ratios, are needed to externally validate these prediction models, the findings provide proof of concept that risk score-based screening programs could be developed to avert severe malaria cases in early childhood.