bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alpha-tubulin enhanced renal tubular cell proliferation and tissue repair but reduced cell death and cell-crystal adhesion
Authors: Manissorn Juthatip , Khamchun Supaporn , Vinaiphat Arada , Thongboonkerd Visith 
Published: July 2016

AbstractAdhesion of calcium oxalate (CaOx) crystals on renal tubular epithelial cells is a critical event for kidney stone disease that triggers many cascades of cellular response. Our previous expression proteomics study identified several altered proteins in MDCK renal tubular cells induced by CaOx crystals. However, functional significance of those changes had not been investigated. The present study thus aimed to define functional roles of such proteome data. Global protein network analysis using STRING software revealed α-tubulin, which was decreased, as one of central nodes of protein-protein interactions. Overexpression of α-tubulin (pcDNA6.2-TUBA1A) was then performed and its efficacy was confirmed. pcDNA6.2-TUBA1A could maintain levels of α-tubulin and its direct interacting partner, vimentin, after crystal exposure. Also, pcDNA6.2-TUBA1A successfully reduced cell death to almost the basal level and increased cell proliferation after crystal exposure. Additionally, tissue repair capacity was improved in pcDNA6.2-TUBA1A cells. Moreover, cell-crystal adhesion was reduced by pcDNA6.2-TUBA1A. Finally, levels of potential crystal receptors (HSP90, HSP70 and α-enolase) on apical membrane were dramatically reduced to basal levels by pcDNA6.2-TUBA1A. These findings implicate that α-tubulin has protective roles in kidney stone disease by preventing cell death and cell-crystal adhesion, but on the other hand, enhancing cell proliferation and tissue repair function.
Role for the thromboxane A2 receptor β-isoform in the pathogenesis of intrauterine growth restriction
Authors: Powell Katie L. , Stevens Veronica , Upton Dannielle H. , McCracken Sharon A. , Simpson Ann M. , Cheng Yan , Tasevski Vitomir , Morris Jonathan M. , Ashton Anthony W. 
Published: July 2016

AbstractIntrauterine growth restriction (IUGR) is a pathology of pregnancy that results in failure of the fetus to reach its genetically determined growth potential. In developed nations the most common cause of IUGR is impaired placentation resulting from poor trophoblast function, which reduces blood flow to the fetoplacental unit, promotes hypoxia and enhances production of bioactive lipids (TXA2 and isoprostanes) which act through the thromboxane receptor (TP). TP activation has been implicated as a pathogenic factor in pregnancy complications, including IUGR; however, the role of TP isoforms during pregnancy is poorly defined. We have determined that expression of the human-specific isoform of TP (TPβ) is increased in placentae from IUGR pregnancies, compared to healthy pregnancies. Overexpression of TPα enhanced trophoblast proliferation and syncytialisation. Conversely, TPβ attenuated these functions and inhibited migration. Expression of the TPβ transgene in mice resulted in growth restricted pups and placentae with poor syncytialisation and diminished growth characteristics. Together our data indicate that expression of TPα mediates normal placentation; however, TPβ impairs placentation and promotes the development of IUGR and represents an underappreciated pathogenic factor in humans.
Polysaccharide Nanoparticles for Efficient siRNA Targeting in Cancer Cells by Supramolecular pKa Shift
Authors: Zhang Ying-Ming , Yang Yang , Zhang Yu-Hui , Liu Yu 
Published: July 2016

AbstractBiomacromolecular pKa shifting is considered as one of the most ubiquitous processes in biochemical events, e.g., the enzyme-catalyzed reaction and protein conformational stabilization. In this paper, we report on the construction of biocompatible polysaccharide nanoparticle with targeting ability and lower toxicity by supramolecular pKa shift strategy. This was realized through a ternary assembly constructed by the dual host‒guest interactions of an adamantane-bis(diamine) conjugate (ADA) with cucurbit[6]uril (CB[6]) and a polysaccharide. The potential application of such biocompatible nanostructure was further implemented by the selective transportation of small interfering RNA (siRNA) in a controlled manner. It is demonstrated that the strong encapsulation of the ADA’s diammonium tail by CB[6] not only reduced the cytotoxicity of the nano-scaled vehicle but also dramatically enhanced cation density through an obvious positive macrocycle-induced pKa shift, which eventually facilitated the subsequent siRNA binding. With a targeted polysaccharide shell containing a cyclodextrin‒hyaluronic acid conjugate, macrocycle-incorporated siRNA polyplexes were specifically delivered into malignant human prostate PC-3 cells. The supramolecular polysaccharide nanoparticles, the formation of which was enabled and promoted by the complexation-assisted pKa shift, may be used as a versatile tool for controlled capture and release of biofunctional substrates.
Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo
Authors: Kamezaki Aosa , Sato Fuminori , Aoki Kazuhiro , Asakawa Kazuhide , Kawakami Koichi , Matsuzaki Fumio , Sehara-Fujisawa Atsuko 
Published: July 2016

AbstractNeuregulin1 (NRG1) plays diverse developmental roles and is likely involved in several neurological disorders including schizophrenia. The transmembrane NRG1 protein is proteolytically cleaved and released as a soluble ligand for ErbB receptors. Such post-translational processing, referred to as ‘ectodomain shedding’, is thought to be crucial for NRG1 function. However, little is known regarding the regulatory mechanism of NRG1 cleavage in vivo. Here, we developed a fluorescent probe, NRG1 Cleavage Indicating SenSOR (N-CISSOR), by fusing mCherry and GFP to the extracellular and intracellular domains of NRG1, respectively. N-CISSOR mimicked the subcellular localization and biochemical properties of NRG1 including cleavage dynamics and ErbB phosphorylation in cultured cells. mCherry/GFP ratio imaging of phorbol-12-myristate-13-acetate-stimulated N-CISSOR-expressing HEK293T cells enabled to monitor rapid ectodomain shedding of NRG1 at the subcellular level. Utilizing N-CISSOR in zebrafish embryos revealed preferential axonal NRG1 ectodomain shedding in developing motor neurons, demonstrating that NRG1 ectodomain shedding is spatially regulated at the subcellular level. Thus, N-CISSOR will be a valuable tool for elucidating the spatiotemporal regulation of NRG1 ectodomain shedding, both in vitro and in vivo.
First Person Perspective of Seated Participants Over a Walking Virtual Body Leads to Illusory Agency Over the Walking
Authors: Kokkinara Elena , Kilteni Konstantina , Blom Kristopher J. , Slater Mel 
Published: July 2016

AbstractAgency, the attribution of authorship to an action of our body, requires the intention to carry out the action, and subsequently a match between its predicted and actual sensory consequences. However, illusory agency can be generated through priming of the action together with perception of bodily action, even when there has been no actual corresponding action. Here we show that participants can have the illusion of agency over the walking of a virtual body even though in reality they are seated and only allowed head movements. The experiment (n = 28) had two factors: Perspective (1PP or 3PP) and Head Sway (Sway or NoSway). Participants in 1PP saw a life-sized virtual body spatially coincident with their own from a first person perspective, or the virtual body from third person perspective (3PP). In the Sway condition the viewpoint included a walking animation, but not in NoSway. The results show strong illusions of body ownership, agency and walking, in the 1PP compared to the 3PP condition, and an enhanced level of arousal while the walking was up a virtual hill. Sway reduced the level of agency. We conclude with a discussion of the results in the light of current theories of agency.
ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells
Authors: Morandi E. M. , Verstappen R. , Zwierzina M. E. , Geley S. , Pierer G. , Ploner C. 
Published: July 2016

AbstractThe fate of human adipose tissue stem cells (ASCs) is largely determined by biochemical and mechanical cues from the extracellular matrix (ECM), which are sensed and transmitted by integrins. It is well known that specific ECM constituents influence ASC proliferation and differentiation. Nevertheless, knowledge on how individual integrins regulate distinct processes is still limited. We performed gene profiling of 18 alpha integrins in sorted ASCs and adipocytes, identifying downregulations of RGD-motif binding integrins integrin-alpha-V (ITGAV) and integrin-alpha-5 (ITGA5), upregulation of laminin binding and leukocyte-specific integrins and individual regulations of collagen and LDV-receptors in differentiated adipocytes in-vivo. Gene function analyses in in-vitro cultured ASCs unraveled differential functions of ITGA5 and ITGAV. Knockdown of ITGAV, but not ITGA5 reduced proliferation, caused p21Cip1 induction, repression of survivin and specific regulation of Hippo pathway mediator TAZ. Gene knockdown of both integrins promoted adipogenic differentiation, while transgenic expression impaired adipogenesis. Inhibition of ITGAV using cilengitide resulted in a similar phenotype, mimicking loss of pan-ITGAV expression using RNAi. Herein we show ASC specific integrin expression patterns and demonstrate distinct regulating roles of both integrins in human ASCs and adipocyte physiology suggesting a negative impact of RDG-motif signaling on adipogenic differentiation of ASCs via ITGA5 and ITGAV.
Oxidative DNA damage stalls the human mitochondrial replisome
Authors: Stojkovič Gorazd , Makarova Alena V. , Wanrooij Paulina H. , Forslund Josefin , Burgers Peter M. , Wanrooij Sjoerd 
Published: July 2016

AbstractOxidative stress is capable of causing damage to various cellular constituents, including DNA. There is however limited knowledge on how oxidative stress influences mitochondrial DNA and its replication. Here, we have used purified mtDNA replication proteins, i.e. DNA polymerase γ holoenzyme, the mitochondrial single-stranded DNA binding protein mtSSB, the replicative helicase Twinkle and the proposed mitochondrial translesion synthesis polymerase PrimPol to study lesion bypass synthesis on oxidative damage-containing DNA templates. Our studies were carried out at dNTP levels representative of those prevailing either in cycling or in non-dividing cells. At dNTP concentrations that mimic those in cycling cells, the replication machinery showed substantial stalling at sites of damage and these problems were further exacerbated at the lower dNTP concentrations present in resting cells. PrimPol, the translesion synthesis polymerase identified inside mammalian mitochondria, did not promote mtDNA replication fork bypass of the damage. This argues against a conventional role for PrimPol as a mitochondrial translesion synthesis DNA polymerase for oxidative DNA damage; however, we show that Twinkle, the mtDNA replicative helicase, is able to stimulate PrimPol DNA synthesis in vitro, suggestive of an as yet unidentified role of PrimPol in mtDNA metabolism.
CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing
Authors: Dehairs Jonas , Talebi Ali , Cherifi Yacine , Swinnen Johannes V. 
Published: July 2016

AbstractThe advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones.
Unbreakable distributed storage with quantum key distribution network and password-authenticated secret sharing
Authors: Fujiwara M. , Waseda A. , Nojima R. , Moriai S. , Ogata W. , Sasaki M. 
Published: July 2016

AbstractDistributed storage plays an essential role in realizing robust and secure data storage in a network over long periods of time. A distributed storage system consists of a data owner machine, multiple storage servers and channels to link them. In such a system, secret sharing scheme is widely adopted, in which secret data are split into multiple pieces and stored in each server. To reconstruct them, the data owner should gather plural pieces. Shamir’s (k, n)-threshold scheme, in which the data are split into n pieces (shares) for storage and at least k pieces of them must be gathered for reconstruction, furnishes information theoretic security, that is, even if attackers could collect shares of less than the threshold k, they cannot get any information about the data, even with unlimited computing power. Behind this scenario, however, assumed is that data transmission and authentication must be perfectly secure, which is not trivial in practice. Here we propose a totally information theoretically secure distributed storage system based on a user-friendly single-password-authenticated secret sharing scheme and secure transmission using quantum key distribution and demonstrate it in the Tokyo metropolitan area (≤90 km).
Effects of neonatal stress on gamma oscillations in hippocampus
Authors: Dricks Sally 
Published: July 2016

AbstractChronic early life stress increases adult risk for depression, bipolar disorder and schizophrenia, illnesses characterized by aberrant functions of cognition and memory. We asked whether chronic early life stress disrupts maturation of gamma oscillations, on which these functions depend. Lifelong impairment of the stress response results from separation of rat pups from the dam for three hours per day during a critical period of hippocampal development (PNDs 2–14). Parvalbumin-expressing interneurons, including the basket cell network which is fundamental to gamma oscillations, are reduced in number in post mortem studies of bipolar disorder and schizophrenia and in chronically-stressed adult rats. To determine effects of chronic early life stress on gamma oscillations, we separated pups from dams once each day on PNDs 2–14 and recorded in vitro at PNDs 15–21. In control pups, separated for 15 minutes per day, gamma power had highly significant correlations with both age (p = 0.0022) and weight (p = 0.0024); gamma in pups separated for 180 minutes per day was not correlated with either factor. ANCOVA indicated significant differences between the groups in both measures. These findings indicate that chronic early life stress can disrupt maturation of the gamma oscillation network.
The metabolome of [2-14C](−)-epicatechin in humans: implications for the assessment of efficacy, safety and mechanisms of action of polyphenolic bioactives
Authors: Ottaviani Javier I. , Borges Gina , Momma Tony Y. , Spencer Jeremy P. E. , Keen Carl L. , Crozier Alan , Schroeter Hagen 
Published: July 2016

AbstractDiet is a major life style factor affecting human health, thus emphasizing the need for evidence-based dietary guidelines for primary disease prevention. While current recommendations promote intake of fruit and vegetables, we have limited understanding of plant-derived bioactive food constituents other than those representing the small number of essential nutrients and minerals. This limited understanding can be attributed to some extent to a lack of fundamental data describing the absorption, distribution, metabolism and excretion (ADME) of bioactive compounds. Consequently, we selected the flavanol (−)-epicatechin (EC) as an example of a widely studied bioactive food constituent and investigated the ADME of [2-14C](−)-epicatechin (300 μCi, 60 mg) in humans (n = 8). We demonstrated that 82 ± 5% of ingested EC was absorbed. We also established pharmacokinetic profiles and identified and quantified >20 different metabolites. The gut microbiome proved to be a key driver of EC metabolism. Furthermore, we noted striking species-dependent differences in the metabolism of EC, an insight with significant consequences for investigating the mechanisms of action underlying the beneficial effects of EC. These differences need to be considered when assessing the safety of EC intake in humans. We also identified a potential biomarker for the objective assessment of EC intake that could help to strengthen epidemiological investigations.
Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae)
Authors: Jia Xiao-Jian , Wang Hai-Xiang , Yan Zeng-Guang , Zhang Min-Zhao , Wei Chun-Hua , Qin Xiao-Chun , Ji Wei-Rong , Falabella Patrizia , Du Yan-Li 
Published: July 2016

AbstractThe yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putative odorant receptors (ORs) and 7 putative ionotropic receptors (IRs) were annotated and identified as olfactory-related genes of C. punctiferalis. Further analysis of RT-qPCR revealed that all these olfactory genes are primarily or uniquely expressed in male and female antennae. Among which, 3 OBPs (OBP4, OBP8 and PBP2) and 4 ORs (OR22, OR26, OR44 and OR46) were specially expressed in male antennae, whereas 4 ORs (OR5, OR16, OR25 and OR42) were primarily expressed in female antennae. The predicted protein sequences were compared with homologs in other lepidopteran species and model insects, which showed high sequence homologies between C. punctiferalis and O. furnacalis. Our work allows for further functional studies of pheromone and general odorant detection genes, which might be meaningful targets for pest management.
“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens
Authors: Hu Shengbiao , Liu Zhengqiang , Zhang Xu , Zhang Guoyong , Xie Yali , Ding Xuezhi , Mo Xiangtao , Stewart A. Francis , Fu Jun , Zhang Youming , Xia Liqiu 
Published: July 2016

AbstractHeterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to efficiently clone gene clusters directly from genomic DNA and several approaches have been developed. Here, we present an alternative strategy based on the site-specific recombinase system Cre/loxP for direct cloning gene clusters. A type three secretion system (T3SS) gene cluster (~32 kb) from Photorhabdus luminescens TT01 and DNA fragment (~78 kb) containing the siderophore biosynthetic gene cluster from Agrobacterium tumefaciens C58 have been successfully cloned into pBeloBAC11 with “Cre/loxP plus BAC” strategy. Based on the fact that Cre/loxP system has successfully used for genomic engineering in a wide range of organisms, we believe that this strategy could be widely used for direct cloning of large DNA fragment.
Augmenting College Students' Study of Speech-Language Pathology Using Computer-Based Mini Quiz Games
Authors: Vinney Lisa A. , Howles Les , Leverson Glen , Connor Nadine P. 
Published: July 2016

Purpose
This study examined whether undergraduate college students' immediate recall and longer-term retention of introductory voice disorder concepts improved by using mini quiz games (MQGs; interactive knowledge tests in game format) compared with (a) traditional study alone, (b) MQGs and traditional study together, or (c) a no-study control condition.

Method
Ninety-three college students participated in proctored sessions in which they were given a pretest, viewed an online lecture on introductory voice disorder concepts, and then engaged in either no intervention or interventions including traditional study, MQG play, or both MQG play and traditional study, followed by an immediate recall posttest and longer-term retention follow-up test.

Results
Analyses suggested that the effects of all interventions (traditional study, MQG play, and the combination of the 2) were equivalent and resulted in significantly greater improvements from pretest to immediate recall posttest performance than the control condition. In contrast, MQGs and MQGs with traditional study, but not traditional study alone, showed better results for long-term retention than no study.

Conclusion
Results provide preliminary support for the idea that there may be multiple effective learning modes, beyond traditional study, that enhance recall and retention of knowledge foundational to speech-language pathology clinical training and practice.
Errata: Die Zukunft der medizinisch–rehabilitativen Versorgung im Kontext der Multimorbidität – Teil I: Begriffsbestimmung, Versorgungsfragen und Herausforderungen
Authors: Seger W. , Cibis W. , Deventer A. , Grotkamp S. , Lübke N. , Schönle P.-W. , Schmidt-Ohlemann M. , Schubert M. 
Published: July 2016

W. Seger, W. Cibis, A. Deventer, S. Grotkamp, N. Lübke, P.-W. Schönle, M. Schubert. Die Zukunft der medizinisch-rehabilitativen
Versorgung im Kontext der Multimorbidität – Teil I: Begriffsbestimmung, Versorgungsfragen und Herausforderungen Künstlerische
Therapeuten im Gesundheitswesen. Gesundheitswesen 2016;
Bei diesem Beitrag fehlte ein Autor und die zugehörige Institutsangabe.
Nachfolgend die ergänzten Autoren und Institute:
Autoren:

W. Seger1, W. Cibis2, A. Deventer3, S. Grotkamp4, N. Lübke5,
P.-W. Schönle6, M. Schmidt-Ohlemann7, M. Schubert2

Institute:

1 MDK Niedersachsen, Hannover
2 Bundesarbeitsgemeinschaft für Rehabilitation (BAR),
Frankfurt
3 Praxis für Physikalische und Rehabilitative Medizin,
Hamburg
4 MDK Niedersachsen, SEG 1, Hannover
5 Kompetenz-Centrum Geriatrie (KCG), Hamburg
6 Maternus Kliniken, Bad Oeynhausen
7 Diakonie Bad Kreuznach, Rehabilitationsklinik, Bad Kreuznach
Final report on EURAMET.QM-S10/1274: supplementary comparison of preparative capabilities for automotive gas mixtures
Authors: Val'ková M , Ďurišová Z , Szilágyi Z N , Büki T , Fükű J 
Published: July 2016

This bilateral supplementary preparative comparison involves standard gas mixtures of automotive gas containing carbon monoxide, carbon dioxide and propane in nitrogen.
Two laboratories (SMU, Slovakia and MKEH, Hungary) participated in this supplementary comparison. SMU was the coordinating laboratory, responsible for collecting and reporting measurement results. The participants have established facilities for automotive gas gravimetric preparation and analysis.
The agreement of the results in this supplementary comparison is good. All the results with their reported uncertainties are in agreement with the reference values for the participants. SMU participated and obtained good results in the previous preparative comparison organised within EURAMET in this field. Both laboratories have existing claims for their Calibration and Measurement Capabilities (CMCs) for automotive gas mixtures.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).