bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Tetraspanins at Cell Interfaces: Functional Modulation and Exosome‐Based Drug Delivery for Precise Disease Treatment
Authors: Xu Kun , Feng Huixia , Zhao Rui , Huang Yanyan 
Published: October 2024

AbstractTetraspanins are key players in various physiological and pathological processes, including malignancy, immune response, fertilization, and infectious disease. Affinity ligands targeting the interactions between tetraspanins and partner proteins are promising for modulating downstream signaling pathways, thus emerging as attractive candidates for interfering related biological functions. Due to the involvement in vesicle biogenesis and cargo trafficking, tetraspanins are also regarded as exosome markers, and become molecular targets for drug loading and delivery. Given the rapid development in these areas, this minireview focuses on recent advances in design and engineering of affinity binders toward tetraspanins including CD63, CD81, and CD9. Their mechanism of actions in modulating protein interactions at cell interfaces and treatment of malignant diseases are discussed. Strategies for constructing exosome‐based drug delivery platforms are also reviewed, with emphasis on the important roles of tetraspanins and the affinity ligands. Finally, challenges and future development of tetraspanin‐targeting therapy and exosomal drug delivery platforms are also discussed.
Lack of Concentration‐QTc Relationship and Cardiac Risk With Vatiquinone Therapeutic and Supratherapeutic Doses
Authors: Lee Lucy , Flach Stephen , Xue Hongqi , Arivelu Lavanya , Golden Lee , Kong Ronald , Darpo Borje 
Published: October 2024

AbstractVatiquinone, a 15‐lipoxygenase inhibitor, is in development for patients with Friedreich ataxia. The study determined the effect of vatiquinone on electrocardiogram parameters. This was a 2‐part, single‐center, randomized, double‐blinded, and placebo‐controlled study. Part 1 used an adaptive approach to identify a supratherapeutic dose, while Part 2 evaluated the effect of vatiquinone on Fridericia corrected QT interval (QTcF). A safe and tolerated supratherapeutic dose of 1400 mg was identified. Concentration‐QTcF analysis confirmed there was no statistically significant relationship between vatiquinone concentration and QTcF. QTcF effect (ie, ΔΔQTcF) exceeding 10 milliseconds was excluded for concentrations up to approximately 11,500 ng/mL. By‐time‐point analysis confirmed that least‐squares mean ΔΔQTcF was below 10 milliseconds. Largest least‐squares mean ΔΔQTcF of 1.5 milliseconds was observed at 2 hours after dosing. Vatiquinone did not have a clinically relevant effect on heart rate nor on cardiac conduction (PR interval and QRS interval). No new safety signals were found, as safety data are consistent with the known safety profile of vatiquinone. These findings altogether demonstrated that there is a minimal cardiac risk for vatiquinone concentrations up to the supratherapeutic dose level.
Bioequivalence Analysis of Clindamycin Hydrochloride Capsules in Healthy Chinese Subjects Under Fasted and Fed Conditions
Authors: Wang Zhi , Wen Haitang , Qian Xuebing , Huang Min , Cheng Guohua , Zhong Guoping 
Published: October 2024

AbstractClindamycin is a lincosamide antibiotic for the treatment of staphylococcal, streptococcal, and anaerobic bacterial infections. We conducted a single‐center, single‐dose, 2‐preparation, 2‐period, 2‐sequence, randomized, open‐label, 2 × 2 crossover study to evaluate the pharmacokinetics (PKs) and safety of the test and reference clindamycin hydrochloride capsules in healthy Chinese subjects under both fasted and fed conditions. Forty‐eight subjects were enrolled in the study totally, with 24 subjects in each group. All subjects were asked to take a test or reference preparation, under either fasted or fed condition, and their blood samples were collected and assayed by a validated high‐performance liquid chromatography‐tandem mass spectrometry method. PK parameters including maximum plasma concentration, area under the plasma concentration‐time curve from time 0 to the last concentration, and area under the plasma concentration‐time curve from time 0 to infinity were estimated with noncompartmental model and analyzed. Results showed that the PK profiles of the 2 preparations were consistent and met the bioequivalence criteria. Food was identified as a factor that had an impact on clindamycin absorption. The safety of clindamycin hydrochloride capsules was satisfactory. This study proved that the 2 clindamycin hydrochloride capsules were bioequivalent in healthy Chinese subjects under both fasted and fed conditions.
Unveiling Eco‐Friendly Reverse Micelle Systems: Dimethyl Carbonate as a Novel Biocompatible Solvent
Authors: González Herrera Alejandra , Mariano Correa N. , Dario Falcone R. , Moyano Fernando 
Published: October 2024

AbstractIn this study, we systematically explored the characteristics of dimethyl carbonate (DMC)/sodium 1,4‐bis‐2‐ethylhexylsulfosuccinate (AOT) reverse micelles (RMs) in the presence of water using dynamic light scattering (DLS), proton nuclear magnetic resonance (1H NMR), and molecular probes. DMC, a biocompatible solvent, enables the formulation of AOT RMs without the need for a co‐surfactant. DLS revealed that as the water content increased, the droplet sizes grew larger. 1H NMR studies indicated that at low water content, water molecules interacted with DMC via hydrogen bonding. This interaction promoted the penetration of DMC toward the interface, affecting the solvation of AOT's sulfonate group. At higher water content, a competition for hydrogen bonding emerged between water‐water and water‐surfactant molecules, leading to distinct interfacial properties, as evidenced by molecular probes. The critical micellar concentration for DMC/AOT/water RMs was 7×10−3 M, similar to RMs formed with other biocompatible solvents. The presence of water facilitated the solvation of the surfactant's polar regions, promoting the RMs formation. The polarity of this system was measured using the ET(30) value. This novel micellar system holds significant potential in various fields, including catalysis, nanomaterials, and green chemistry.
The impact of Big Data Analytics on firm sustainable performance
Authors: Ertz Myriam , Latrous Imen , Dakhlaoui Ahlem , Sun Shouheng 
Published: October 2024

AbstractThis study evaluates the impact of Big Data Analytics (BDA) on firm sustainable performance (FSP). BDA is conceptualized as a dual construct comprising predictive and prescriptive analytics, while FSP is considered from a triple bottom line (TBL) perspective comprising the economic, social, and environmental lines of firm performance. The study relies exclusively on independent third‐party BDA and FSP data pertaining to 522 firms from the US S&P500 Index and the Canadian S&P500/TSX60 Index. The data is analyzed with ordinary least squares (OLS) regression, and the findings reveal, on aggregate, that BDA has a direct, positive, and significant effect on overall FSP. The results of the piecemeal analysis show that BDA is positively related to the economic, social, and environmental dimensions. Furthermore, our distinction between predictive and prescriptive analytics suggests that prescriptive analytics outperforms the FSP results obtained with predictive analytics moderately. The study insights provide strategic knowledge for firms seeking to leverage digitalization for enhanced corporate citizenship while boosting their digital capabilities. The impact of technology, especially Big Data, on sustainability, has gained traction in the literature, yet this is the first study to delve deeper into the detailed relationships between both constructs by deciphering and quantifying the impact of BDA components on the TBL.
Seeing is believing: Do face‐to‐face meetings between independent directors and investors enhance corporate ESG performance?
Authors: Liu Qi , Qin Shuai , Chen Chen 
Published: October 2024

AbstractThis article explores the topic of whether face‐to‐face communication between independent directors and investors can help them obtain firm‐specific information and promote management to improve corporate environmental, social, and governance (ESG) performance. Our findings revealed a notable positive correlation between the frequency of independent directors attending shareholder meetings (IDASM) and ESG performance. The underlying mechanism for this correlation is that IDASM increases investment in research and development (R&D) and green patent applications, which can drive long‐term firm value. Further analysis reveals that independent directors attending annual shareholder meetings can more effectively enhance a company's ESG performance. Independent directors attending shareholder meetings also exhibits a more noticeable impact on improving corporate governance performance than environment performance and social performance. The effect becomes more pronounced when independent directors possess an accounting background, have shorter tenures, when the meetings attended by more minority shareholders, the degree of enterprise information asymmetry is high. The research results have reference significance for improve the information disadvantage position of independent directors, restrain short‐sighted behavior of management, and improving the role of shareholder meetings in protecting investor interests.
Pb‐N Chemically Anchors CsPbBr3 on 1D Porous Tubular g‐C3N4 for Enhanced Photocatalytic CO2 Reduction
Authors: Li Dong , Wang Kaixuan , Chen Ying , Li Renyi , Tang Jiahong , Zhao Yizhou , Guo Wei , Chen Qi , Li Yujing 
Published: October 2024

AbstractHalide perovskite CsPbBr3 (CPB) quantum dots (QDs) are considered as a promising candidate for solar‐driven CO2 conversion for their unique optoelectronic properties and suitable band structures. However, the CO2 conversion efficiency of pristine CsPbBr3 quantum dots is severely limited due to their rapid electron‐hole pair recombination and insufficient active sites. In this study, by immobilizing CPB QDs onto one‐dimensional (1D) porous tubular g‐C3N4 (TCN), an effective 0D/1D CPB@TCN heterojunction photocatalyst for CO2 reduction is fabricated. Density functional theory (DFT) calculations combined with experimental studies demonstrate that CPB QDs are uniformly anchored on the surface of TCN through Pb−N chemical bonding, resulting in efficient separation and transfer of photogenerated carriers in CPB@TCN photocatalysts. The resultant CPB@TCN photocatalysts exhibit significantly enhanced photocatalytic activity for CO2 reduction with the highest conversion rate of 22.62 μmol g−1 h−1, which is 5.33‐times higher than that of pristine CPB QDs. This work unveils the interfacial bonding mechanism of CPB@TCN heterojunction through Pb−N chemical bond, which provides new insights for the development perovskite‐based heterojunction photocatalysts.
Insight into prostate cancer osteolytic metastasis by RelB coordination of IL‐8 and S100A4
Authors: Sun Wenbo , Xu Kenny , Li Xiao , Qian Peipei , Xu Fan , Zhang Yanyan , Wang Xiumei , Xu Zhi , Ding Jiaji , Xu Xinyu , Wei Xiaowei , Jiang Qin , Xu Yong 
Published: October 2024

AbstractBackgroundAlthough RANK‐LRANK interaction is essential for osteoclastogenesis, the mechanisms by which cancer cells invade bone tissues and initiate osteolytic metastasis remain unclear. Here, we show that the hyperactivation of RelB fosters prostate cancer (PCa) osteolytic metastasis by coordinating interleukin‐8 (IL‐8) and calcium‐binging protein A4 (S100A4).MethodsThe factors promoting PCa bone metastasis were investigated in sera from PCa patients and tumour tissues derived from nude mice using immunohistochemical analysis and enzyme‐linked immunosorbent assays (ELISA). Cell mobility and mineralization were quantified using BioStation CT and Osteolmage assay. The relative cistrome was investigated in advanced PCa cells by standard transcriptional analyses, including the luciferase reporter response, site‐directed mutagenesis, and chromatin immunoprecipitation (ChIP) assay. PCa cell‐initiated tumour formation, expansion, and bone metastasis were validated in mice using multiple approaches, including orthotopic, intraskeletal, and caudal arterial implantation models.ResultsIL‐8 and S100A4 correlated with patient Gleason scores and bone metastasis. RelB upregulated IL‐8, facilitating androgen receptor (AR)‐independent growth. RelB‐Sp1 interaction enhanced epithelial‐mesenchymal transition (EMT) by activating Snail and Twist. RelB‐NFAT1c super‐enhancer upregulated S100A4 in the organization of the cytoskeleton and bone metastasis. The RelB‐IL‐8‐S100A4 signalling axis was confirmed to promote osteolytic metastasis in nude mice.ConclusionRelB‐IL‐8 reciprocally promoted EMT by activating inflammatory signalling and inactivating AR signalling. IL‐8 is essential for provoking PCa metastasis but insufficient to drive bone metastasis. IL‐8‐S100A4 cooperation was necessary for metastatic cells to target the bone.Highlights
RelB activates inflammatory signalling by upregulating IL‐8 and suppressing AR.
RelB upregulates S100A4 by cooperating with NFATC1.
IL‐8 boosts EMT by activating Snail 1 and Twist 1, and S100A4 exacerbates osteolytic metastasis via calcium consumption.
RelB harnesses IL‐8 and S100A4 to drive PCa osteolytic metastasis.
The role and advance of ubiquitination and deubiquitination in depression pathogenesis and treatment
Authors: Yan Xiaoru , Ma Yunhui , Yang Junting , Chang Xiaoqi , Shi Shuxuan , Song Guohua 
Published: October 2024

AbstractDepression is a common neuropsychiatric disease that is characterized by long‐term, repeated low mood, pain and despair, pessimism, and even suicidal tendencies. Increasing evidence has shown that ubiquitination and deubiquitination are closely related to the occurrence of depression, including pathological morphogenesis, neuroplasticity, synaptic transmission, neuroinflammation, and so forth. The development of depression is regulated by intracellular proteins that undergo various posttranslational modifications, including ubiquitination, which falls under the epigenetics category. Although there have been studies and reviews of literature on epigenetics and depression, a systematic review of ubiquitination modification and depression has not been reported. In addition, with the deepening of research on depression and ubiquitination, the development of drugs targeting the ubiquitin system has gradually increased, but it is still not mature, so there is an urgent need to find new antidepressant drug targets. E3 ubiquitin ligases and deubiquitinating enzymes can regulate the occurrence and development of depression in a variety of ways, which may be a direction for the treatment of depression in the future. Therefore, this review describes the latest progress of ubiquitination and deubiquitination in the regulation of depression, summarizes the published signal pathways of ubiquitination and deubiquitination involved in depression, emphasizes the targets and mechanisms of E3 ubiquitin ligases and deubiquitinase in the regulation of depression, and further discusses the therapeutic targets of targeting ubiquitination modification systems to regulate depression.
Propofol‐alone sedative efficacy in observational biliopancreatic endoscopic ultrasound
Authors: Kato Hisaki , Kawasaki Yuki , Sumi Kazuya , Shibata Yuki , Nomura Norihiro , Ushio Jun , Eguchi Junichi , Ito Takayoshi , Inoue Haruhiro 
Published: October 2024

AbstractObjectivesAppropriate sedative and analgesic selection is essential to reduce patient discomfort and body movement to safely conduct endoscopic ultrasonography (EUS). However, few cases have examined sedation with propofol in EUS, and few studies the need for combined analgesia. In this study, we retrospectively evaluated the usefulness and safety of propofol without analgesics for sedation in biliopancreatic observational EUS.MethodsThis single‐center retrospective study included 516 observational biliopancreatic EUS procedures using propofol alone performed between April 2021 and March 2023. The primary and secondary endpoints were the observational biliopancreatic EUS results obtained with propofol alone and adverse event occurrence, respectively.ResultsThe median examination time and total propofol dose were 22 (range: 10–67) min and 186.5 (range: 50–501) mg, respectively. The median starting Richmond Agitation‐Sedation Scale and Visual Analog Scale scores were −5 (range: −5–1) and 0 (range: 0–10), respectively. The median recovery time was 22 (range: 5–80) min. Adverse events occurred in 60 (11.6%) patients. Trainee‐performed examination (odds ratio [OR] 3.52, 95% confidence interval [CI]: 1.63–7.60, p = 0.0014) and examination length (>22 min; OR 1.67, 95% CI: 0.95–2.92, p = 0.07) were risk factors for adverse events.High body mass index (OR 1.87, 95% CI: 1.10–3.16, p = 0.02) and extended examination time (OR 4.23, 95% CI: 2.08–8. 57, p < 0.001) were risk factors for delayed recovery.ConclusionsDuring observational biliopancreatic EUS, propofol is useful as a single sedative and offers high patient satisfaction and relative safety.
Accounting for effects of growth rate when measuring ecological stability in response to pulse perturbations
Authors: Mentges Andrea , Clark Adam Thomas , Blowes Shane A. , Kunze Charlotte , Hillebrand Helmut , Chase Jonathan M. 
Published: October 2024

AbstractEcological stability is a vital component of natural ecosystems that can inform effective conservation and ecosystem management. Furthermore, there is increasing interest in making comparisons of stability values across sites, systems and taxonomic groups, often using comparative synthetic approaches, such as meta‐analysis. However, these synthetic approaches often compare/contrast systems where measures of stability mean very different things to the taxa involved. Here, we present results from theoretical models and empirical data to illustrate how differences in growth rates among taxa influence four widely used metrics of ecological stability of species abundances responding to pulse perturbations: resilience, recovery, resistance and temporal stability. We refer to these classic growth‐rate‐dependent metrics as ‘realised’ stability. We show that realised resilience and realised temporal stability vary as a function of organisms' growth rates; realised recovery depends on the relation between growth rate and sampling duration; and realised resistance depends on the relation between growth rate and sampling interval. To account for these influences, we introduce metrics intended to be more independent of growth rates, which we refer to as ‘intrinsic’ stability. Intrinsic stability can be used to summarise the overall effects of a disturbance, separately from internal recovery processes – thereby allowing more general comparisons of disturbances across organisms and contexts. We argue that joint consideration of both realised and intrinsic stability is important for future comparative studies.
Improving Inference Within Freshwater Community Studies: Accounting for Variable Detection Rates of Amphibians and Fish
Authors: Hamer Andrew J. , Horányi Júlia 
Published: October 2024

ABSTRACTResearch into freshwater communities often aims to link patterns of species distribution in ponds with underlying biotic factors. However, errors with species detection (e.g. false negatives) may underestimate distribution and bias assessments of community structure. Occupancy models that account for imperfect detection offer a solution to this problem. Here, we used three methods (call/visual encounter surveys, dip‐netting and newt trapping) to survey amphibians and fish (potential amphibian predators) at 100 ponds in an urbanised landscape in Hungary over one breeding season. We estimated species detection probabilities for amphibians (all life stages combined) and fish using occupancy models to gain insight into amphibian‐fish relationships and other survey‐specific variables. We detected nine amphibian and 20 fish species. There were relatively low but variable estimated probabilities of detection for amphibians (mean: 0.320, 95% Bayesian credible interval: 0.142–0.598), with three species having detection rates < 0.1. Probabilities of detection peaked in the middle of the breeding season and increased with survey effort. Detection probabilities of five species were negatively associated with the detection of fish at a pond, while there were positive relationships between detection and emergent vegetation cover. We found no substantial differences in detection rates among the three survey methods. The probability of detecting fish was much higher than for amphibians (0.588, 0.503–0.717) but was lower at ponds with high emergent vegetation where amphibian detection was higher. Our results underscore the importance of accounting for the imperfect detection of both response organisms and potentially interacting species in aquatic community studies. We recommend applying multi‐species occupancy models to enable inference for both common and rare species at ponds in landscapes subjected to human disturbances.
Post‐Copulatory Sexual Selection in an Insect With High Levels of Mating Failure
Authors: Balfour Vicki L. , Armand Mélissa , Shuker David M. 
Published: October 2024

ABSTRACTSexual selection is not a single process. Instead, multiple processes of sexual selection can interact with respect to a given phenotype, in either reinforcing, independent, or conflicting directions. Here we consider how different processes of sexual selection interact in the seed bug Lygaeus simulans. This species is characterised by limited pre‐copulatory sexual selection, but the potential for rather strong post‐copulatory sexual selection. In particular, mating failure is common in this species, with around 40%–60% of copulations failing to result in the successful transfer of sperm. Mating failure is negatively correlated with female size, with smaller females being less likely to end up inseminated. We have recently argued that this pattern is best explained by cryptic male mate choice for large, more fecund females. Males therefore preferentially inseminate larger females. Here we explore how this potential cryptic male choice interacts with another component of post‐copulatory sexual selection: sperm competition. We first manipulated male and female size variation, generating large and small, male and female, size classes. Using a visible mutant marker to assign paternity, we then double‐mated females with males, in all combinations of male and female size. Our results showed that sperm competition outcomes were primarily driven by copulation duration, with longer copulations leading to greater paternity share for a male. We also confirmed that larger females are more likely to produce offspring than smaller females, as predicted by cryptic male choice for large females. This effect was again linked to copulation duration, with longer copulations less likely to lead to mating failure. While larger males tended to be more successful in sperm competition, especially if copulating second, female size had little effect on paternity, suggesting that cryptic male choice and sperm competition are acting relatively independently in this species.