bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between empirical dietary inflammatory pattern and risk of cancer and cancer-specific mortality: a systematic review and meta-analysis of prospective cohort studies
Authors: Hosseini Fatemeh S. , Nikparast Ali , Etesami Elahe , Javaheri-Tafti Fatemeh , Asghari Golaleh 
Published: October 2024

Background/aimCurrent evidence indicates a correlation between the inflammatory potential of diet and the risk of cancer and cancer-specific mortality. This study aimed to assess the association between empirical dietary inflammatory pattern (EDIP), which has recently been designed based on the inflammatory potential of the diet, and the risk of cancer and cancer-specific mortality.MethodsA systematic literature search was conducted across the PubMed/Medline, Scopus, and Web of Science databases from January 2016 to March 2024. A random effects model was used to calculate the pooled effect size (ES) and 95% confidence intervals (95% CI). Heterogeneity between studies was assessed using the Cochran Q test and the I2 statistic.ResultsFrom the initial 229 records, 24 prospective cohort studies with 2,683,350 participants and 37,091 cancer incidence cases, as well as 20,819 cancer-specific mortality, were included in our study. Pooled results indicated a significant association between higher adherence to the EDIP and an increased risk of total cancer (ES: 1.10; 95% CI: 1.05–1.15; I2 = 41.1), colorectal cancer (ES: 1.19; 95% CI: 1.11–1.27; I2 = 41.1), and liver cancer (ES: 1.48; 95% CI: 1.14–1.94; I2 = 36.9). However, no significant association between increased adherence to the EDIP and an increased risk of ovarian or endometrial cancer was found. Furthermore, greater adherence to the EDIP was significantly associated with an increased risk of cancer-specific mortality (ES: 1.18; 95% CI: 1.05–1.33; I2 = 45.4).ConclusionOur results showed that a diet with higher inflammatory properties is associated with an increased risk of cancer and cancer-specific mortality.Systematic review registrationPROSPERO registration no. CRD42024496912.
Jiajiejian gel ameliorates thyroid nodules through regulation of thyroid hormones and suppression of the (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway
Authors: Wang Changlin , Gao Xiangju , Qiao Mingqi , Gao Dongmei , Guo Yinghui , Wang Jieqiong , Song Chunhong 
Published: October 2024

BackgroundThe high incidence of thyroid nodules and their rapid growth in recent years have become an important issue affecting public health. Traditional Chinese medicine (TCM) external treatments have unique advantages in treating this disease, but the currently available external preparations are relatively few and the therapeutic mechanism is unclear. Jiajiejian gel (JJJG) is a TCM external preparation developed by our team for the thyroid nodule treatment, which has been preliminarily proven to be safe and effective in clinical practice.ObjectiveThe current study was aimed to elucidate the therapeutic effects and the underlying mechanisms of JJJG on thyroid nodules in rats.MethodsThe contents of paeonol and forsythoside A in JJJG were determined by HPLC. The thyroid nodules rat model was established through oral gavage of 0.1% propylthiouracil (PTU) for 6 weeks and meanwhile the rats were treated with external JJJG (0.26, 0.52, 1.04 g/kg). Subsequently, the therapeutic effect of JJJG was observed by means of ultrasonic examination, morphology observation, organ coefficients determination and histopathological analysis. Mechanismlly, the levels of FT3, FT4 and TSH in serum were measured and transcriptomics methods were used to analyse and screen the key targets and pathways of alleviating thyroid nodules by JJJG. Further, gene and protein expression levels of key factors in the pathways were measured and validated using quantitative real-time PCR, ELISA, western blotting and immunofluorescence, so as to clarify the therapeutic mechanism.ResultsThe contents of the paeonol and forsythoside A were 1.160 and 0.608 mg/g, respectively. JJJG reduced thyroid swelling, improved nodular lesions, decreased thyroid coefficients, and inhibited abnormal nodular hyperplasia of follicular epithelial cells. In terms of mechanism, JJJG significantly increased the levels of FT3 and FT4 and decreased TSH level in serum (P &lt; 0.05). Transcriptomics suggested that the (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway may be one of the key mechanisms in the treatment of thyroid nodules by JJJG. Further validation experiments demonstrated that JJJG significantly reduced the mRNA expression and protein content of IL-1β, IL-6 and TNF-α in thyroid tissue, as well as the mRNA expression of JAK2, STAT3 and VEGF and the protein expression of p-JAK2/JAK2, p-STAT3/STAT3 and VEGF (P &lt; 0.05).ConclusionThis study indicates that JJJG efficiently ameliorates thyroid nodules by regulating the levels of FT3, FT4 and TSH in serum and suppressing (IL-6, TNF-α, IL-1β)/JAK2/STAT3/VEGF pathway in thyroid tissue, providing a potential therapeutic approach for thyroid nodules.
Monocrotaline-mediated autophagy via inhibiting PI3K/AKT/mTOR pathway induces apoptosis in rat hepatocytes
Authors: Guo Yazhou , Yuan Yang , Wang Ruibo , Bai Jun , Jia Yanqing , Qiu Xinxin , Niu Huafeng , Li Long , Luo Yan , Zhao Baoyu , Zhang Zhencang 
Published: October 2024

Monocrotaline (MCT), a major pyrrolizidine alkaloid, is well-known for its high liver toxicity. Dysregulation of autophagy induced apoptosis can lead to various liver diseases, including those induced by chemical compounds. Therefore, we aim to explore whether autophagy might serve as a potential strategy for addressing liver apoptosis caused by MCT. In primary rat hepatocytes (PRHs), MCT significantly increased the number of autophagosomes and the expression levels of LC3II, Becline-1, and Atg5, while it decreased the expression of p62 in a concentration-dependent manner at doses of 100, 200, 300, and 400 μM. Western blot assays revealed MCT inhibited the phosphorylation levels of the PI3K/AKT/mTOR pathway. To elucidate the role of autophagy in mediating MCT-induced apoptosis, we further pretreated PRHs with the autophagy agonist Rapamycin and the inhibitors Bafilomycin A1 and Chloroquine, respectively, and assessed the apoptosis of PRHs induced by MCT. The results displayed that Rapamycin increased the apoptosis rate and the expression of cleaved caspase-3, whereas Bafilomycin A1 and Chloroquine reduced the apoptosis and the expression of cleaved caspase-3 in PRHs. This study confirms that autophagy enhances PRHs apoptosis induced by MCT. In summary, this study demonstrates that MCT-induced autophagy via inhibition of the PI3K/AKT/mTOR pathway can lead to apoptosis in PRHs.
Transient changes in L-arginine, asymmetric and symmetric dimethyl arginine in triathletes following Norseman Xtreme Triathlon
Authors: Bonnevie-Svendsen Martin , Nyborg Christoffer , Bratseth Vibeke , Melau Jørgen , Hisdal Jonny 
Published: October 2024

Arterial vasodilation is dependent on nitric oxide synthesized from L-arginine by endothelial nitric oxide synthase. Triathletes are reported to display altered serum concentrations of nitric oxide metabolites such as L-arginine, asymmetric dimethyl arginine (ADMA) and symmetric dimethyl arginine (SDMA) shortly after completing long-distance triathlon races. In other populations, similar changes to nitric oxide metabolites are established risk markers of cardiovascular disease. The objective of this study was to assess serum concentrations of metabolites for endothelial nitric oxide synthesis in triathletes one week following a long-distance triathlon race. In this prospective observational study, we used high-performance liquid chromatography to measure circulating concentrations of L-arginine, ADMA, and SDMA in triathletes. Venous blood samples were collected before, immediately after, day one, and one week following the triathlon race. Serum concentrations and L-arginine/ADMA ratio were determined for each time-point and compared to baseline. L-arginine/ADMA ratio was reduced on day one (147 ± 32 vs 163 ± 40, p &lt; 0.02). ADMA was reduced immediately after and increased at day one and remained elevated at one week (0.29 ± 0.05 μM, p &lt; 0.001, 0.44 ± 0.08 μM, p &lt; 0.001 and 0.42 ± 0.07 μM, p = 0.04, respectively vs 0.40 ± 0.05 μM). SDMA was increased at all time-points when compared to baseline (0.48 ± 0.10 μM, p &lt; 0.001, 0.53 ± 0.11 μM, p &lt; 0.001 and 0.42 ± 0.08 μM, p = 0.048 vs 0.38 ± 0.05 μM). L-arginine was only decreased immediately after (46.0 ± 9.3 μM vs. 64.6 ± 16.1 μM, p &lt; 0.001). Long-distance triathlon racing induces altered levels of metabolites for endothelial nitric oxide production that mostly normalizes within one week following racing. The clinical relevance of these transient changes has yet to be elucidated in the athletic population.
Higher fraction of inspired oxygen during anesthesia increase the risk of postoperative pulmonary complications in patients undergoing non-cardiothoracic surgery: a retrospective cohort study
Authors: Wang Tianzhu , Zhao Weixing , Ma Libin , Wu Jing , Ma Xiaojing , Liu Luyu , Cao Jiangbei , Lou Jingsheng , Mi Weidong , Zhang Changsheng 
Published: October 2024

ObjectiveThe ideal intra-operative inspired oxygen concentration remains controversial. We aimed to investigate the association between the intraoperative fraction of inspired oxygen (FiO2) and the incidence of postoperative pulmonary complications (PPCs) in patients undergoing non-cardiothoracic surgery.MethodsThis was a retrospective cohort study of elderly patients who underwent non-cardiothoracic surgery between April 2020 and January 2022. According to intraoperative FiO2, patients were divided into low (≤60%) and high (&gt;60%) FiO2 groups. The primary outcome was the incidence of a composite of pulmonary complications (PPCs) within the first seven postoperative days. Propensity score matching (PSM) and inverse probability treatment weighting (IPTW) were conducted to adjust for baseline characteristic differences between the two groups. Multivariate logistic regression analysis was used to calculate the odds ratios (OR) for FiO2 and PPCs.ResultsAmong the 3,515 included patients with a median age of 70 years (interquartile range: 68–74), 492 (14%) experienced PPCs within the first 7 postoperative days. Elevated FiO2 was associated with an increased risk of PPCs in all the logistic regression models. The OR of the FiO2 &gt; 60% group was 1.252 (95%CI, 1.015–1.551, P = 0.038) in the univariate analysis. In the multivariate logistic regression models, the ORs of the FiO2 &gt; 60% group were 1.259 (Model 2), 1.314 (Model 3), and 1.32 (model 4). A balanced covariate distribution between the two groups was created using PSM or IPTW. The correlation between elevated FiO2 and an increased risk of PPCs remained statistically significant with PSM analysis (OR, 1.393; 95% CI, 1.077–1.804; P = 0.012) and IPTW analysis (OR, 1.266; 95% CI, 1.086–1.476; P = 0.003).ConclusionHigh intraoperative FiO2 (&gt;60%) was associated with the postoperative occurrence of pulmonary complications, independent of predefined risk factors, in elderly non-cardiothoracic surgery patients. High intraoperative FiO2 should be applied cautiously in surgical patients vulnerable to PPCs.
Molecular mechanism of GIRK2 channel gating modulated by cholesteryl hemisuccinate
Authors: Cui Meng , Lu Yongcheng , Ma Xinyi , Logothetis Diomedes E. 
Published: October 2024

Cholesterol, an essential lipid of cell membranes, regulates G protein-gated inwardly rectifying potassium (GIRK) channel activity. Previous studies have shown that cholesterol activates GIRK2 homotetrameric channels, which are expressed in dopaminergic neurons of the brain. Deletion of GIRK2 channels affects both GIRK2 homo- and heterotetrames and can lead to abnormal neuronal excitability, including conditions such as epilepsy and addiction. A 3.5 Å cryo-EM structure of GIRK2 in complex with CHS (cholesteryl hemisuccinate) and PIP2 (phosphatidylinositol 4,5-bisphosphate) has been solved. This structure provides the opportunity to study GIRK2 channel gating dynamics regulated by cholesterol using gating molecular dynamics (GMD) simulations. In the present study, we conducted microsecond-long GMD simulations on the GIRK2 channel in its APO, PIP2, and PIP2/CHS bound states, followed by systematic analysis to gain molecular insights into how CHS modulates GIRK2 channel gating. We found that CHS binding facilitates GIRK2 channel opening, with 43 K+ ion permeation events observed, compared to 0 and 2 K+ ion permeation events for GIRK2-APO and GIRK2/PIP2, respectively. Binding of CHS to the GIRK2 channel enhances PIP2 and channel interactions, which is consistent with previous experimental results. The negatively charged PIP2 alters the internal electrostatic potential field in the channel and lowers the negative free energy barrier for K+ ion permeation.
A feasibility and usability study of a virtual reality tool (VESPA 2.0) for cognitive rehabilitation in patients with mild cognitive impairment: an ecological approach
Authors: Latella Desirèe , Formica Caterina , Ielo Augusto , Grioli Pietro , Marra Angela , Costanzo Daniela , Merlo Maria Emanuele , Pappalardo Salvatore Marco , Corallo Francesco , Marino Silvia , Quartarone Angelo , Calabrò Rocco Salvatore , Maresca Giuseppa 
Published: October 2024

IntroductionMild cognitive impairment (MCI) or “mild neurocognitive disorder” represents an intermediate status between normality and dementia. It is characterized by cognitive decline that does not significantly interfere with normal daily living activities. Virtual reality (VR) is the new frontier of rehabilitation.MethodsWe enrolled 50 MCI patients who underwent a neuropsychological evaluation and participated in 40 sessions of cognitive treatment using the Virtual Environment for a Superior Neuro-Psychiatry, Second Generation (VESPA 2.0) System. This preliminary study highlights the role of VR tools for cognitive rehabilitation (CR) for the recovery of cognitive functions and consequent better management of MCI condition. Our study demonstrated that the VESPA 2.0 System is a valuable tool in a context that closely resembles real-life situations rather than controlled, artificial environments as traditional cognitive training methods.ResultsThe results showed that the patient group had significant improvements between T0 and T1 (assessment), in particular, in the global cognitive profile, visuospatial skills, and executive functions after treatment with the VESPA 2.0 System.DiscussionOur findings contribute with new evidence of understanding the impact of using simulations of the Activities of Daily Living (ADL) scale in the CR.
Pattern and associated factors of COVID-19 knowledge, attitude and practice (KAP) among COVID-19-comorbid patients: a systematic review and meta-analysis
Authors: Rehman Anees ur , Tasleem Zermina , Muhammad Sohail Ayaz , Rasool Muhammad Fawad , Shah Shahid , Jabeen Gul , Arif Sonia , Omar Babkair Lujain Salim , Kadumi Yahya Jaber , Alghamdi Saleh , Almarzoky Abuhussain Safa S. , Khayyat Sarah M. , Alharthi Raed Hamed Hilal , Hossain Mohammad Akbar , Abbas Asma A. , Elrggal Mahmoud Essam , Haseeb Abdul 
Published: October 2024

BackgroundThe COVID-19 comorbid population is at higher risk of developing severe health issues like acute respiratory distress syndrome, coagulation syndrome, metabolic acidosis, and septic shock, potentially leading to patient death. Patients’ knowledge, attitudes, and practices (KAP) significantly influence their response to the pandemic and aid in enhancing health policy implementation.ObjectiveTo identify and evaluate the pattern and associated factors to COVID-19 knowledge, attitude, and practice among individuals with comorbidities.MethodologyThe systematic review followed the PRISMA guidelines. Relevant studies assessing the KAP of comorbid patients were retrieved by carefully searching the PubMed and Google Scholar databases. The appraisal tool for cross-sectional studies was used to determine the quality of the included studies and the risk of biases.ResultsEighteen studies met the inclusion criteria and were included in the review. The pooled sample size of the included studies was 9,104. Different comorbidities reported in the studies include hypertension, diabetes, psychological disorders, and cancer. Pooled analysis showed that 65% of patients showed good knowledge, 57% of patients showed a positive attitude and 51% of patients followed good practices to manage the COVID-19 in presence of their comorbid condition. Significant factors impacting knowledge, attitude and practice in COVID-19 comorbid patients were ethnicity OR 1.78 [95% CI 1.35–2.32]; educational status 3.2 [2.79–3.58]; urban residence 2.43 [1.65–3.02]; employment Status 1.67[1.34–2.12]; financial Status 4.02[3.66–4.38]; occupation 3.65[3.31–4.25]; information Source 2.64[2.19–3.26]; comorbidity 3.28[2.78–3.61]; and duration of chronic illness 1.59[1.31–2.04].ConclusionComorbid COVID-19 patients showed good knowledge, positive attitude and good practice towards the management of the disease.
Assessment of abdominal obesity prevalence and determinants among adults in southwest Ethiopia: a cross-sectional study
Authors: Yosef Tewodros , Sebeta Asresash , Tekalign Eyob , Sisay Binyam Girma , Bekele Bayu Begashaw , Tesfaw Aragaw , Shifera Nigusie 
Published: October 2024

BackgroundAbdominal obesity, excess fat around the abdomen, is more harmful than other fat types and is a key diagnostic criterion for metabolic syndrome. It poses a growing global public health concern. This study aimed to assess the prevalence of and determinants of abdominal obesity among adults in southwest Ethiopia.MethodsA cross-sectional study involving 624 adults in Semen Bench district, southwest Ethiopia was conducted from May 23 to June 23, 2022. The data was gathered using questionnaires and anthropometric measurements. The data were analyzed using Stata version 18. A robust Poisson regression was used due to the abdominal obesity prevalence exceeding 10%. Variables with p-values &lt; 0.25 in the bivariate analysis were included in the multivariable analysis. The adjusted prevalence ratio (APR) and 95% confidence interval were reported to indicate statistical significance and the strength of associations.ResultsThe prevalence of abdominal obesity was 18% (95% CI: 15–21%). After adjusting for confounding variables, the determinants of abdominal obesity included participants aged 31–50 (APR = 3.62, 95% CI: 2.67–8.95) and 51–65 (APR = 3.24, 95% CI: 2.51–8.69), being female (APR = 2.65, 95% CI: 1.63–4.98), having a low wealth index (APR = 1.46, 95% CI: 1.19–3.76), physical inactivity (APR = 1.39, 95% CI: 1.06–4.18), lack of adequate dietary diversity (APR = 3.08, 95% CI: 2.09–6.42), and food insecurity (APR = 2.84, 95% CI: 1.82–7.68).ConclusionThe research revealed that 18% of the participants exhibited abdominal obesity. Factors such as advanced age, being female, having a low wealth index, physical inactivity, insufficient dietary diversity, and food insecurity were identified as contributors to abdominal obesity. Therefore, it is crucial to develop targeted interventions to address modifiable factors, as this can also help prevent the onset of non-communicable chronic diseases associated with abdominal obesity.
Long-Term Exposure to Nitrogen Dioxide and Ozone and Mortality: Update of the WHO Air Quality Guidelines Systematic Review and Meta-Analysis
Authors: Kasdagli Maria-Iosifina , Orellano Pablo , Pérez Velasco Román , Samoli Evangelia 
Published: October 2024

ObjectivesWe performed a systematic review and meta-analysis on long-term exposure to nitrogen dioxide (NO2) and ozone (O3) with mortality, to expand evidence that informed 2021 the WHO Air Quality Guidelines and guide the Health Risks of Air Pollution in Europe project.MethodsWe included cohorts investigating NO2 and O3 mortality from all-causes, respiratory diseases, chronic obstructive pulmonary disease (COPD), acute lower respiratory infections (ALRI); and NO2 mortality from circulatory, ischemic heart, cerebrovascular diseases and lung cancer. We pooled estimates by random-effects models and investigated heterogeneity. We assessed the certainty of the evidence using the Grading of Recommendations Assessment Development approach and Evaluation (GRADE).ResultsWe selected 83 studies for NO2 and 26 for O3 for the meta-analysis. NO2 was associated with all outcomes, except for cerebrovascular mortality. O3 was associated with respiratory mortality following annual exposure. There was high heterogeneity, partly explained by region and pollutant levels. Certainty was high for NO2 with COPD and ALRI, and annual O3 with respiratory mortality.ConclusionAn increasing body of evidence, with new results from countrywide areas and the Western Pacific, supports certainty, including new outcomes.
Diagnosis of auditory disorders in dental patients with altered occlusal relationships (Part 1)
Authors: Gaivoronskaya A. A. , Tsimbalistov A. V. , Voityatskaya I. V. , Lopushanskaya T. А. , Chernogaeva E. A. , Solovykh E. A. 
Published: October 2024

Relevance. Recently, dental patients have been increasingly presenting with not only aesthetic, functional, and painrelated concerns, but also various otological symptoms, such as ear congestion, discomfort, and perceived hearing loss. These symptoms may be linked to underlying dental conditions, especially those involving a reduction in interalveolar distance (IAD), and must be carefully differentiated from ENT disorders and true hearing impairment.Objective: To evaluate the auditory function in dental patients with partial tooth loss and complaints of pathological otological symptoms.Materials and methods. The study included 357 dental patients aged 43 to 72 years, comprising 287 women (80.4%) and 70 men (19.6%) with partial tooth loss. The examination involved a functional-physiological method for determining the central relationship of the jaws using the Apparatus for Determining Central Occlusion (AOTSO) and tonal threshold audiometry.Results. A reduction in interalveolar height of varying degrees was confirmed in 325 (91%) patients with dental arch defects, while no decrease was observed in 23 (9%) patients. All patients with otological complaints exhibited a reduction in lower facial height. Of the 87 patients with otological complaints, 37 (42.5%) were diagnosed with significant hearing loss based on tonal threshold audiometry.Conclusion. The examination of this group of dental patients with dental arch defects, utilizing modern diagnostic methods, confirmed a reduction in interalveolar distance. Additionally, otological complaints were identified, and pathological auditory conditions, such as various forms of hearing loss, were verified through tonal threshold audiometry.