bluuebunny/crossref_metadata_2025_split huggingface dataset:
Method Modification Study for the Thermo Scientific SureTect™ Listeria Species Assay–Matrix Extension
Authors: Cloke Jonathan , Evans Katharine , Crabtree David , Hughes Annette , Simpson Helen , Holopainen Jani , Wickstrand Nina , Kauppinen Mikko , Leon-Velarde Carlos , Larson Nathan , Dave Keron , Chen Yi , Ryser Elliot , Carter Mark 
Published: March 2016

Abstract
The Thermo Scientific™ SureTect™ Listeria species assay is a new real-time PCR assay for the detection of all species of Listeria in food and environmental samples. The assay was originally certified as Performance Tested MethodsSM (PTM) 071304 in 2013. This report details the method modification study undertaken to extend the performance claims of the assay for matrixes of raw ground turkey, raw ground pork, bagged lettuce, raw pork sausages, pasteurized 2% fat milk, raw cod, pasteurized brie cheese, and ice cream. The method modification study was conducted using the AOAC Research Institute (RI) PTM program to validate the SureTect PCR assay in comparison to the reference method detailed in ISO 11290-1:1996 including amendment 1:2004. All matrixes were tested by Thermo Fisher Scientific (Basingstoke, United Kingdom). In addition, three matrixes (raw cod, bagged lettuce, and pasteurized brie cheese) were analyzed independently as part of the AOAC RI-controlled independent laboratory study by the University of Guelph, Canada. Using probability of detection statistical analysis, there was no significant difference in the performance between the SureTect assay and the International Organization for Standardization reference method for any of the matrixes analyzed in this study.
Representações, valores e crenças em discursos de professores da educação básica e implicações na (form)ação docente
Authors: Barros Ev Angela Batista Rodrigues de 
Published: March 2016

<p class="Padro">Como importante política pública, o investimento na iniciação à docência, por meio de um programa específico – o PIBID – tem conquistado alguns resultados promissores na reaproximação entre futuros docentes e a escola pública. Na Universidade em que atuo, esse programa tem por princípio norteador ampliar a interlocução entre a academia e a educação básica, propiciando a todos os seus participantes momentos de estudo, discussão e enriquecimento pessoal, cultural e profissional, por meio das diversas atividades. Passando por momentos que vão desde a idealização, planejamento e execução dos projetos de intervenção, além da reflexão sobre os problemas enfrentados no cotidiano e ao retorno mais concertado à ação pedagógica, tem possibilitado emergir aspectos importantes relativos à docência, promovendo uma desautomatização de práticas e concepções pedagógicas, além de uma desnaturalização de procedimentos, permitindo enxergar sobre o modo de enxergar a si e à realidade, tanto no âmbito profissional como mais amplamente. Como culminância, tem fomentado e possibilitado a participação dos envolvidos em seminários (internos e externos) com apresentação dos trabalhos realizados. Neste artigo, serão discutidas algumas representações sociais (Moscovici, 2003), constitutivas do <em>ethos </em> da profissão docente, a partir dos discursos de cinco docentes da área de Letras, professoras do ensino médio da rede pública estadual,. Para Larrosa (2002), somos "seres viventes com palavras", vivemos media(tiza)dos pela palavra. Dessa forma, as respostas trazidas à discussão, a partir dos questionários aplicados, esboçam discurso(s) dos docentes sobre como se percebem e ao seu fazer pedagógico e delineiam-se sentidos e sentimentos atribuídos ao seu espaço de atuação, a escola básica.</p>
O direito à angústia e à felicidade em Luuanda
Authors: Salgado Maria Teresa 
Published: March 2016

<p>Nos 50 anos de aniversário da obra Luuanda, de Luandino Vieira, considerada uma das mais importantes da ficção angolana, propomos uma reflexão sobre a sua atualidade. Partimos da ideia de que a angústia que atravessa todos os contos merece ser "escutada". Nossa leitura propõe um análise psicossocial implicada da obra, seguindo as sugestões de João Frayze Pereira; partimos dos detalhes que os contos nos oferecem, ao descortinar não só a violência e a angústia, mas também as suas consequências. Pensamos nos recalques, atualizados nas situações oníricas, nos chistes e em outros aspectos do reprimido. Além disso, propomos uma leitura de Luuanda em confronto com o que se vive 50 anos depois de sua elaboração, isto é, o que se vive hoje na cena social angolana.</p><p>Palavras chave- angústia, felicidade, Luandino Vieira, Luuanda, análise psicossocial implicada</p><p> </p>
The intertext of modernism in Crônica da casa assassinada, de Lúcio Cardoso
Authors: Redmond William Valentine 
Published: March 2016

<p><strong>Abstract</strong></p><p>This article reflects on the many opinions about the author of the of <strong>Crônica da casa assassinada</strong> as a modernist or postmodernist writer. Examining the narrative techniques used by the author, maybe influenced by his wide reading of foreign literatures <strong>,</strong> it may be possible to clarify a little the question of the position of Lucio Cardoso in relation to the literary movements of the twentieth century. His personal experience as a writer passes through the process of impersonalisation, transforming his masterpiece into a universal symbol. It is hoped that this paper will show how this transformation of his personal experience comes across to the readers in the form of a modernist and not as some say, a post modernist novel.</p><p> </p><p><strong>Resumo:</strong><strong> </strong></p><p>Este artigo reflete sobre as muitas opiniões acerca do autor da <strong>Crônica da casa assassinada</strong> como um escritor modernista ou pós-modernista. Examinando as técnicas narrativas utilizadas pelo autor, talvez influenciado por sua vasta leitura de literaturas estrangeiras relatadas, seria possível clarear um pouco esta questão da posicionamento de Lucio Cardoso com relação aos movimentos literários do século XX. A experiência pessoal do escritor passa por um processo de impessoalidade, através da transmutação e amalgamação, transformando a obra em símbolo universal. Espera-se com esta comunicação discutir o modo como essa transmutação chega ao leitor em forma de um romance modernista e não, como às vezes se afirma, como um romance pós-modernista.</p><p> </p>
Phase diagram and polarization of stable phases of (Ga1−
xInx)2O3
Authors: Maccioni Maria Barbara , Fiorentini Vincenzo 
Published: March 2016

Abstract
The full phase diagram of (Ga1−

x
In
x
)2O3 is obtained theoretically. The phases competing for the ground state are monoclinic β (low x), hexagonal (x ∼ 0.5), and bixbyite (large x). Three disconnected mixing regions interlace with two distinct phase-separation regions, and at x ∼ 0.5, the coexistence of hexagonal and β alloys with phase-separated binary components is expected. We also explore the permanent polarization of the phases, but none of them are polar. On the other hand, we find that ε-Ga2O3, which was stabilized in recent experiments, is pyroelectric with a large polarization and piezoelectric coupling, and could be used to produce high-density electron gases at interfaces.
Emerging Radiometals forPETImaging
Authors: Nedrow Jessie R. , Anderson Carolyn J. 
Published: March 2016

AbstractRadiopharmaceuticals are drugs containing a radionuclide and are utilized for diagnosis, monitoring, and/or treatment of diseases. This article focuses on the development of emerging radiometal‐based imaging agents for positron emission tomography (PET), as well as new developments in F‐18‐labeled agents via aluminum fluoride chemistry. The incorporation of radiometals through chelation is typically simpler than the common multistep reactions to incorporate F‐18 into molecules, and radiometal‐conjugates generally require minimal if any purification. Widely studied PET radiometals (e.g., gallium‐68, copper‐64, zirconium‐89, yttrium‐86, and rubidium‐82) can be readily produced and have desirable decay properties for PET imaging. However, there are several emerging radiometals with desirable properties for PET imaging (e.g., scandium‐44, multiple isotopes of copper, terbium‐182, gallium‐66, and cobalt‐55) with unique properties (e.g., a range of half‐lives) that provide additional opportunities for PET imaging and allow for the developmental of new and unique PET radiopharmaceuticals.
Synthesis of Planar Chiral Shvo Catalysts for Asymmetric Transfer Hydrogenation
Authors: Dou Xiaowei , Hayashi Tamio 
Published: March 2016

AbstractA new type of planar chiral Shvo catalysts, where the chirality is based solely on different substitution flanking the CO function, was prepared and used for transfer hydrogenation of imines and ketones. The reduction of ketimines represented by N‐(1‐phenylethylidene)aniline and prochiral ketones such as phenyl trifluoromethyl ketone with 2‐propanol was efficiently catalyzed by 0.5 mol% of the chiral Shvo catalyst to give high yields of the corresponding reduction products with the enantioselectivities in the range 45% to 64% ee.magnified image
Organocatalytic Enantioselective Conjugate Addition of Malonic Acid Half Thioesters to Coumarin‐3‐carboxylic Acids Using N‐Heteroarenesulfonyl Cinchona Alkaloid Amides
Authors: Nakamura Shuichi , Toda Ayaka , Sano Masahide , Hatanaka Tsubasa , Funahashi Yasuhiro 
Published: March 2016

AbstractWe disclose herein an efficient enantioselective conjugate addition reaction between coumarin‐3‐carboxylic acids and malonic acid half thioesters (MAHTs). The reaction was catalyzed by N‐heteroarenesulfonyl Cinchona alkaloid amides to afford double‐decarboxylative conjugate addition products in good yield with high enantioselectivity. The reaction of various coumarin‐3‐carboxylic acids with MAHTs gave products in high yield with high enantioselectivity.magnified image
Nondisruptive Dissolution of Hyperpolarized 129Xe into Viscous Aqueous and Organic Liquid Crystalline Environments
Authors: Truxal Ashley E. , Slack Clancy C. , Gomes Muller D. , Vassiliou Christophoros C. , Wemmer David E. , Pines Alexander 
Published: March 2016

AbstractStudies of hyperpolarized xenon‐129 (hp‐129Xe) in media such as liquid crystals and cell suspensions are in demand for applications ranging from biomedical imaging to materials engineering but have been hindered by the inability to bubble Xe through the desired media as a result of viscosity or perturbations caused by bubbles. Herein a device is reported that can be reliably used to dissolve hp‐129Xe into viscous aqueous and organic samples without bubbling. This method is robust, requires small sample volumes (<60 μL), is compatible with existing NMR hardware, and is made from readily available materials. Experiments show that Xe can be introduced into viscous and aligned media without disrupting molecular order. We detected dissolved xenon in an aqueous liquid crystal that is disrupted by the shear forces of bubbling, and we observed liquid‐crystal phase transitions in (MBBA). This tool allows an entirely new class of samples to be investigated by hyperpolarized‐gas NMR spectroscopy.
A Stable Heterocyclic Amino(phosphanylidene‐σ4‐phosphorane) Germylene
Authors: Del Rio Natalia , Baceiredo Antoine , Saffon‐Merceron Nathalie , Hashizume Daisuke , Lutters Dennis , Müller Thomas , Kato Tsuyoshi 
Published: March 2016

AbstractA new stable heterocyclic germylene, in which the divalent germanium atom lies between a nitrogen atom and a phosphanylidene phosphorane group, was synthesized. Experimental and theoretical studies revealed the peculiar effect of phosphanylidene phosphorane substituent, which is a stronger π‐donor towards germanium than an amino group is. Because of the weak phosphorus–germanium π‐bond, this new germylene compound shows an enhanced reactivity compared to classical N‐heterocyclic germylenes.
Asymmetric Brønsted Acid Catalyzed Substitution of Diaryl Methanols with Thiols and Alcohols for the Synthesis of Chiral Thioethers and Ethers
Authors: Chatupheeraphat Adisak , Liao Hsuan‐Hung , Mader Steffen , Sako Makoto , Sasai Hiroaki , Atodiresei Iuliana , Rueping Magnus 
Published: March 2016

AbstractAn enantioselective addition of thiols and alcohols to aza‐ortho‐quinone methides, starting from diaryl methanols, was developed. The asymmetric additions occur under mild reaction conditions in the presence of chiral phosphoric acids and furnish the corresponding adducts with excellent yields and enantioselectivities.
Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyltransferase MoeN5
Authors: Zhang Lilan , Chen Chun‐Chi , Ko Tzu‐Ping , Huang Jian‐Wen , Zheng Yingying , Liu Weidong , Wang Iren , Malwal Satish R. , Feng Xinxin , Wang Ke , Huang Chun‐Hsiang , Hsu Shang‐Te Danny , Wang Andrew H.‐J. , Oldfield Eric , Guo Rey‐Ting 
Published: March 2016

AbstractThe structure of MoeN5, a unique prenyltransferase involved in the biosynthesis of the antibiotic moenomycin, is reported. MoeN5 catalyzes the reaction of geranyl diphosphate (GPP) with the cis‐farnesyl group in phosphoglycolipid 5 to form the (C25) moenocinyl‐sidechain‐containing lipid 7. GPP binds to an allylic site (S1) and aligns well with known S1 inhibitors. Alkyl glycosides, glycolipids, can bind to both S1 and a second site, S2. Long sidechains in S2 are “bent” and co‐locate with the homoallylic substrate isopentenyl diphosphate in other prenyltransferases. These observations support a MoeN5 mechanism in which 5 binds to S2 with its C6–C11 group poised to attack C1 in GPP to form the moenocinyl sidechain, with the more distal regions of 5 aligning with the distal glucose in decyl maltoside. The results are of general interest because they provide the first structures of MoeN5 and a structural basis for its mechanism of action, results that will facilitate the design of new antibiotics.
Chemoenzymatic epoxidation of Karanja oil: an alternative to chemical epoxidation?
Authors: Bajwa Arjun S. , Sathaye Sadhana , Kulkarni Vaishali M. , Patwardhan Anand V. 
Published: March 2016

AbstractIn light of increasing concern about diminishing fossil fuel reserve, a lot of research is being focussed on the production of chemicals using renewable feedstock. Vegetable oils provide this valuable renewable feedstock as they offer a large number of possibilities. One of the interesting possibilities offered is epoxidized vegetable oil, which can be used as a plasticizer, polyvinyl chloride stabilizer, diluent, etc. Hence, a chemoenzymatic method for epoxidation of Karanja oil was investigated. The enzyme reusability, the important criteria for commercial exploitation of the technique, was also investigated. Three commercially available immobilized lipase enzymes were screened and Novozyme 435 was found to be best for chemoenzymatic epoxidation of Karanja oil. The study revealed that epoxide conversion of 80% (equivalent to chemical methods) was obtained at room temperature in the absence of any side reactions. Maximum epoxide conversion was obtained in 8–9 h at 30 °C as compared with 6 h for conventional methods at 80 °C. The reaction could also be carried out in the absence of any solvent to an extent of 56% oxirane conversion. The catalyst was found to be strongly affected by temperature and H2O2 and denatured after repeated runs, showing a maximal reusability of four runs. The enzymatic method is beyond doubt a cleaner and greener technology compared with conventional method. The advantages of energy efficiency along with 100% selectivity make it a method for the future. Copyright © 2016 Curtin University of Technology and John Wiley & Sons, Ltd.
Combined experimental and numerical kinetic characterization of NR vulcanized with sulfur, N‐terbutyl, 2‐benzothiazylsulfenamide, and N,N‐diphenylguanidine
Authors: Milani G. , Hanel T. , Donetti R. , Milani F. 
Published: March 2016

ABSTRACTA comprehensive experimental and numerical analysis aimed at investigating the rheometer behavior of Natural Rubber (NR) vulcanized with sulfur and two accelerators [N,N‐diphenylguanidine (DPG) and N‐terbutyl, 2‐benzothiazylsulfenamide (TBBS)] is presented. To fit experimental data, the general reaction scheme proposed by Han and co‐workers for vulcanized sulfur NR is re‐adapted and suitably modified, taking into account DPG and TBBS single contributions. An ad hoc interactive software based on GUI technology is utilized to fit experimental data, allowing estimating kinetic constants also by unexperienced users. Chain reactions initiated by the formation of macrocompounds responsible for the formation of the instable crosslinked polymer are accounted for. In the presence of two accelerators, reactions are assumed to proceed in parallel, making the hypothesis of negligible interactions, being their concentrations rather similar. From the kinetic scheme adopted, a closed form solution is found for the crosslinking density, with the only limitation that the induction period is excluded from computations. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43519.
Thermomechanical properties and water uptake capacity of soy protein‐based bioplastics processed by injection molding
Authors: Fernández‐Espada Lucía , Bengoechea Carlos , Cordobés Felipe , Guerrero Antonio 
Published: March 2016

ABSTRACTThe optimization of the processing conditions in the production of soy protein bioplastics by injection molding has been essential in order to develop materials with a great capacity to absorb water while displaying good mechanical properties. Using a 50/50 (wt/wt) soy protein/glycerol mixture, and 40 °C, 500 bar, and 70 °C as reference values for cylinder temperature, injection pressure, and mold temperature, respectively, the effect of those processing parameters over thermomechanical and hydrophilic properties was studied. Processing parameters did not show a great influence over the thermomechanical bending properties within temperatures ranging from −30 to 130 °C, as most samples displayed a similar response, independently of the parameter studied. On the other hand, when studying tensile and hydrophilic properties, the main effect corresponded to the cylinder and mold temperature values, as pressure did not exert a clear influence when increased from 300 to 900 bar. Samples with a lower water uptake were obtained when processed at higher temperature, as a result of crosslinking promotion. Moreover, a greater extensibility was observed when bioplastics are processed at high mold temperatures. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43524.
Improving flame retardancy and melt processability of polyethersulfone using low molecular weight additives
Authors: Owusu‐Nkwantabisah Silas , Staudt Claudia , Lesser Alan J. 
Published: March 2016

ABSTRACTThe effect of a calcium stearate (CaSt2) additive on the melt processability and flame retardancy of polyethersulfone (PES) was studied. Measurements of the viscosity of PES and its composites showed a marked decrease in viscosity with increase in the fraction of CaSt2 additive. About 40% reduction in viscosity of PES was achieved with addition of 5 wt % CaSt2. By decreasing the viscosity, the CaSt2 additive enabled the melt extrusion of PES at lower temperatures up to 90 °C below that of conventional melt processing. The flammability was also investigated using a Pyrolysis Combustion Flow Calorimeter (PCFC). The CaSt2 additive resulted in tremendous improvement in the flame retardancy of PES as evident in the reduction of the heat release capacity (HRC) of the composites by up to 37%. Moreover, the peak of heat release rate (pHRR) of PES in the composites was up to 84% lower than in the neat polymer. The remarkable improvement in flame retardancy of PES was demonstrated to be related to the rapid charring of the composites and the in situ formation of calcium carbonate/calcium oxide upon decomposition of CaSt2. The CaSt2 additive was also found to enhance the flame retardancy of thermoplastics including polyamide‐6 and polypropylene (PP). © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43525.
The MYpop toolbox: Putting yeast stress responses in cellular context on single cell and population scales
Authors: Spiesser Thomas , Kühn Clemens , Krantz Marcus , Klipp Edda 
Published: March 2016

AbstractSystems biology holds the promise to integrate multiple sources of information in order to build ever more complete models of cellular function. To do this, the field must overcome two significant challenges. First, the current strategy to model average cells must be replaced with population based models accounting for cell‐to‐cell variability. Second, models must be integrated with each other and with basic cellular function. This requires a core model of cellular physiology as well as a multiscale simulation platform to support large‐scale simulation of culture or tissues from single cells. Here, we present such a simulation platform with a core model of yeast physiology as scaffold to integrate and simulate SBML models. The software automates this integration helping users simulate their model of choice in context of the cell division cycle. We benchmark model merging, simulation and analysis by integrating a minimal model of osmotic stress into the core model and analyzing it. We characterize the effect of single cell differences on the dynamics of osmoadaptation, estimating when normal cell growth is resumed and obtaining an explanation for experimentally observed glycerol dynamics based on population dynamics. Hence, the platform can be used to reconcile single cell and population level data.
Concentrating membrane proteins using ultrafiltration without concentrating detergents
Authors: Feroz Hasin , Vandervelden Craig , Ikwuagwu Bon , Ferlez Bryan , Baker Carol S. , Lugar Daniel J. , Grzelakowski Mariusz , Golbeck John H. , Zydney Andrew L. , Kumar Manish 
Published: March 2016

ABSTRACTMembrane proteins (MPs) are of rapidly growing interest in the design of pharmaceutical products, novel sensors, and synthetic membranes. Ultrafiltration (UF) using commercially available centrifugal concentrators is typically employed for laboratory‐scale concentration of low‐yield MPs, but its use is accompanied by a concomitant increase in concentration of detergent micelles. We present a detailed analysis of the hydrodynamic processes that control detergent passage during ultrafiltration of MPs and propose methods to optimize detergent passage during protein concentration in larger‐scale membrane processes. Experiments were conducted using nonionic detergents, octyl‐β‐D glucoside (OG), and decyl‐β‐D maltoside (DM) with the bacterial water channel protein, Aquaporin Z (AqpZ) and the light driven chloride pump, halorhodopsin (HR), respectively. The observed sieving coefficient (So), a measure of detergent passage, was evaluated in both stirred cell and centrifugal systems. So for DM and OG increased with increasing filtrate flux and decreasing shear rates in the stirred cell, that is, with increasing concentration polarization (CP). Similar effects were observed during filtration of MP‐detergent (MPD) micelles. However, lower transmission was observed in the centrifugal system for both detergent and MPD systems. This is attributed to free convection‐induced shear and hence reduced CP along the membrane surface during centrifugal UF. Thus to concentrate MPs without retention of detergent, design of UF systems that promote CP is required. Biotechnol. Bioeng. 2016;113: 2122–2130. © 2016 Wiley Periodicals, Inc.