bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antiviral Action of Diphenyl Diselenide on Herpes Simplex Virus 2 Infection in Female BALB/c Mice
Authors: Sartori Gláubia , Jardim Natália Silva , Marcondes Sari Marcel Henrique , Dobrachinski Fernando , Pesarico Ana Paula , Rodrigues Luiz Carlos , Cargnelutti Juliana , Flores Eduardo F. , Prigol Marina , Nogueira Cristina W. 
Published: December 2015

ABSTRACTDiphenyl diselenide, (PhSe)2, is an organoselenium compound with pharmacological actions mostly related to antioxidant and anti‐inflammatory properties. The study investigated its antiviral and virucidal actions against herpes simplex virus 2 (HSV‐2) infection in vitro and in a vaginal infection model in mice. The plaque reduction assay indicated that (PhSe)2 showed virucidal and antiviral actions reducing infectivity in 70.8% and 47%, respectively. The antiviral action of (PhSe)2 against HSV‐2 vaginal infection was performed by infecting mice (105 PFU/ml−1) at day 6. The treatment with (PhSe)2 (5 mg/kg/day, intragastric [i.g.]) followed 5 days before and for more 5 days after infection. The extravaginal lesion score was evaluated from days 6 to 10. At day 11, animals were killed, and histological evaluation, determination of viral load, and TNF‐α and IFN‐γ levels were performed in supernatants of homogenized vaginal tissue. The levels of reactive species (RS), protein carbonyl, non‐protein thiols (NPSH), nitrate/nitrite (NOx), and malondialdehyde (MDA), and the activities of myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined. (PhSe)2 reduced the histological damage, extravaginal lesion scores, the viral load of vaginal tissue, and the activity of MPO, but increased the levels of TNF‐α, IFN‐γ. (PhSe)2 attenuated the increase of RS, MDA, NOx levels and the activity of GR caused by infection. (PhSe)2 also attenuated the reduction of NPSH content and the inhibition of CAT, SOD, and GPx activities. The antiviral action of (PhSe)2 against HSV‐2 infection was related to its immunomodulatory, antioxidant, and anti‐inflammatory properties. J. Cell. Biochem. 117: 1638–1648, 2016. © 2015 Wiley Periodicals, Inc.
Aggregation of Ribosomal Protein S6 at Nucleolus Is Cell Cycle‐Controlled and Its Function in Pre‐rRNA Processing Is Phosphorylation Dependent
Authors: Zhang Duo , Chen Hui‐Peng , Duan Hai‐Feng , Gao Li‐Hua , Shao Yong , Chen Ke‐Yan , Wang You‐Liang , Lan Feng‐Hua , Hu Xian‐Wen 
Published: December 2015

ABSTRACTRibosomal protein S6 (rpS6) has long been regarded as one of the primary r‐proteins that functions in the early stage of 40S subunit assembly, but its actual role is still obscure. The correct forming of 18S rRNA is a key step in the nuclear synthesis of 40S subunit. In this study, we demonstrate that rpS6 participates in the processing of 30S pre‐rRNA to 18S rRNA only when its C‐terminal five serines are phosphorylated, however, the process of entering the nucleus and then targeting the nucleolus does not dependent its phosphorylation. Remarkably, we also find that the aggregation of rpS6 at the nucleolus correlates to the phasing of cell cycle, beginning to concentrate in the nucleolus at later S phase and disaggregate at M phase. J. Cell. Biochem. 117: 1649–1657, 2016. © 2015 Wiley Periodicals, Inc.
Juggling work and family responsibilities when involuntarily working more from home: A multiwave study of financial sales professionals
Authors: Lapierre Laurent M. , van Steenbergen Elianne F. , Peeters Maria C. W. , Kluwer Esther S. 
Published: December 2015

SummaryUsing multiwave survey data collected among 251 financial sales professionals, we tested whether involuntarily working more from home (teleworking) was related to higher time‐based and strain‐based work‐to‐family conflict (WFC). Employees' boundary management strategy (integration vs. segmentation) and work–family balance self‐efficacy were considered as moderators of these relationships. Data were collected one month before, three months after, and 12 months after the implementation of a new cost‐saving policy that eliminated employees' access to office space in a centralized work location. The policy resulted in employees being forced to work more from home. A voluntary telework program had been in effect before the new policy, implying that working more from home as a result of the new policy was involuntary in nature. Results revealed that involuntarily working more from home was associated with higher strain‐based WFC but not higher time‐based WFC. However, moderator analyses revealed that the positive association between involuntarily working more from home and both types of WFC was significantly stronger among employees with weaker self‐efficacy in balancing work and family. Boundary management strategy had no detectable moderating effect. Copyright © 2015 John Wiley & Sons, Ltd.
Projecting spring wheat yield changes on the Canadian Prairies: effects of resolutions of a regional climate model and statistical processing
Authors: Qian Budong , Wang Hong , He Yong , Liu Jiangui , De Jong Reinder 
Published: December 2015

ABSTRACTIn addition to the uncertainty associated with crop models, climate scenarios are still a major source of uncertainty in projecting crop yield changes under climate change. Regional climate models (RCMs) are used as tools for dynamic downscaling of climate scenarios from global climate models (GCMs) to regional scales for climate change impact studies. It is known that running an RCM is more expensive and time consuming at a higher resolution than at a lower resolution. Therefore, it is interesting to investigate how resolutions of an RCM and statistical processing of RCM data may result in differences in projected crop yield changes. We used the decision support system for agrotechnology transfer (DSSAT)–CERES‐Wheat model to simulate yield changes of spring wheat at 13 locations across the Canadian Prairies, with climate scenarios from a Canadian RCM (CanRCM4) driven by a Canadian earth system model (CanESM2) with the forcing scenarios RCP4.5 and RCP8.5 at 25 and 50 km resolutions. Bias correction and a stochastic weather generator referred to as AAFC‐WG were used as statistical processing tools to develop future climate scenarios as input to the crop model. The results showed that when changes were averaged across the locations, whether 25‐ or 50‐km resolution CanRCM4 data were used, the projected yield changes were fairly consistent with those based on its driving GCM–CanESM2, especially if AAFC‐WG was used to develop future climate scenarios. The spatial patterns of the projected yield changes were also similar for the two resolutions of CanRCM4, although the magnitude of the projected changes had a relatively large range among the scenarios at some locations. These results indicated that using future climate scenarios based on climate change simulations by GCMs might be sufficient for projecting regional crop yield changes on the Canadian Prairies.
Triblock Terpolymers by Simultaneous Tandem Block Polymerization (STBP)
Authors: Freudensprung Ines , Klapper Markus , Müllen Klaus 
Published: December 2015

A route of synthesizing triblock terpolymers in a one‐pot, “one‐step” polymerization approach is presented. The combination of two distinct polymerization techniques through orthogonal catalyst/initiator functionalities attached to a polymeric linker furnishes novel pathways to ABC‐terpolymers. Both polymerizations have to be compatible regarding mechanisms, chosen monomers, and solvents. Here, an α,ω‐heterobifunctional poly(ethylene glycol) serves as poly­meric catalyst/initiator to obtain triblock terpolymers of poly(norbornene)‐b‐poly(ethylene glycol)‐b‐poly(l‐lactic acid) PNB‐PEG‐PLLA via simultaneous ring opening metathesis poly­merization and ring opening polymerization in a fast one‐pot polymerization. Structural characterization of the polymers is provided via 1H‐, DOSY‐, and 1H,1H‐COSY‐NMR, while solution and thin film self‐assembly are investigated by dynamic light scattering and atomic force microscopy.
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Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: Reverse engineering development of an anti‐inflammatory cheese
Authors: Plé Coline , Breton Jérôme , Richoux Romain , Nurdin Marine , Deutsch Stéphanie‐Marie , Falentin Hélène , Hervé Christophe , Chuat Victoria , Lemée Riwanon , Maguin Emmanuelle , Jan Gwénaël , Van de Guchte Maarten , Foligné Benoit 
Published: December 2015

Scope: Inflammatory bowel disease (IBD) constitutes a growing public health concern in western countries. Bacteria with anti‐inflammatory properties are lacking in the dysbiosis accompanying IBD. Selected strains of probiotic bacteria with anti‐inflammatory properties accordingly alleviate symptoms and enhance treatment of ulcerative colitis in clinical trials. Such properties are also found in selected strains of dairy starters such as Propionibacterium freudenreichii and Lactobacillus delbrueckii (Ld). We thus investigated the possibility to develop a fermented dairy product, combining both starter and probiotic abilities of both lactic acid and propionic acid bacteria, designed to extend remissions in IBD patients.Methods and results: We developed a single‐strain Ld‐fermented milk and a two‐strain P. freudenreichii and Ld‐fermented experimental pressed cheese using strains previously selected for their anti‐inflammatory properties. Consumption of these experimental fermented dairy products protected mice against trinitrobenzenesulfonic acid induced colitis, alleviating severity of symptoms, modulating local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages. As a control, the corresponding sterile dairy matrix failed to afford such protection.Conclusion: This work reveals the probiotic potential of this bacterial mixture, in the context of fermented dairy products. It opens new perspectives for the reverse engineering development of anti‐inflammatory fermented foods designed for target populations with IBD, and has provided evidences leading to an ongoing pilot clinical study in ulcerative colitis patients.
Diet‐tissue discrimination factors and turnover of carbon and nitrogen stable isotopes in tissues of an adult predatory coral reef fish, Plectropomus leopardus
Authors: Matley J. K. , Fisk A. T. , Tobin A. J. , Heupel M. R. , Simpfendorfer C. A. 
Published: December 2015

RationaleStable isotope ratios (δ13C and δ15N values) provide a unique perspective into the ecology of animals because the isotope ratio values of consumers reflect the values in food. Despite the value of stable isotopes in ecological studies, the lack of species‐specific experimentally derived diet‐tissue discrimination factors (DTDFs) and turnover rates limits their application at a broad scale. Furthermore, most aquatic feeding experiments use temperate, fast‐growing fish species and few have considered medium‐ to large‐sized adults with low growth rates from tropical ecosystems.MethodsA controlled‐diet stable isotope feeding trial was conducted over a 196‐day period for the adult predatory reef fish leopard coralgrouper (Plectropomus leopardus). This study calculated δ13C and δ15N DTDFs and turnover rates in five tissues (liver, plasma, red blood cells (RBC), fin, and muscle) using a continuous flow isotope ratio mass spectrometer equipped with an elemental analyzer. In addition, the effect of chemical lipid extraction (LE) on stable isotope values was examined for each tissue.ResultsTurnover was mainly influenced by metabolism (as opposed to growth) with LE δ15N half‐life values lowest in fin (37 days) and plasma (66 days), and highest in RBC (88 days) and muscle (126 days). The diet‐tissue discrimination factors for δ15N values in all tissues (Δ15N: –0.15 to 1.84‰) were typically lower than commonly reported literature values. Lipid extraction altered both δ15N and δ13C values compared with untreated samples; however, for the δ15N values, the differences were small (mean δ15NLE‐Bulk <0.46‰ in all tissues).ConclusionsThis study informs future interpretation of stable isotope data for medium‐ to large‐sized fish and demonstrates that DTDFs developed for temperate fish species, particularly for δ15N values, may not apply to tropical species. Sampling of muscle and/or RBC is recommended for a relatively long‐term representation of feeding habits, while plasma and/or fin should be used for a more recent indication of diet. Copyright © 2015 John Wiley & Sons, Ltd.
Tandem mass spectrometry sequencing in the negative ion mode to read binary information encoded in sequence‐defined poly(alkoxyamine amide)s
Authors: Charles Laurence , Laure Chloé , Lutz Jean‐François , Roy Raj Kumar 
Published: December 2015

RationaleDigitally encoded oligomers composed of two distinct amide coding units spaced by a nitroxide moiety were recently decrypted using a tandem mass spectrometry (MS/MS) sequencing approach developed for protonated oligomers. Here, the MS/MS behavior of deprotonated oligomers was explored in the negative ion mode to provide both structural and mechanistic complementary information.MethodsBinary‐encoded oligo(alkoxyamine)amides, containing coding 0/1 amide units spaced by a TEMPO nitroxide moiety, were ionized in negative ion mode electrospray thanks to their α end‐group containing a carboxylic acid function. Deprotonated molecules were subjected to collision‐induced dissociation in MS/MS and MS3 experiments, combined with accurate mass measurements, for a thorough investigation of their dissociation behavior.ResultsDeprotonated oligomers readily dissociated upon collisional activation via competitive homolytic cleavages of all fragile alkoxyamine linkages between any coding 0 or 1 monomers and a nitroxide moiety. As expected, only product ions holding the deprotonated α end‐group were detected while complementary moieties containing the ω termination were released as radicals. The so‐formed distonic radical anions were observed to further depolymerize according to a radical‐induced process, as evidenced by MS3 experiments.ConclusionsMessages encoded in oligo(alkoxyamine)amides were readily decrypted by MS/MS sequencing performed in the negative ion mode. When compared with results obtained in positive ion mode ESI‐MS/MS, these data provided further evidence regarding the influence of adducted proton on the charge‐remote homolytic cleavage of alkoxyamine linkages. Copyright © 2015 John Wiley & Sons, Ltd.
Coupling of size‐exclusion chromatography with electrospray ionization charge‐detection mass spectrometry for the characterization of synthetic polymers of ultra‐high molar mass
Authors: Viodé Arthur , Dagany Xavier , Kerleroux Michel , Dugourd Philippe , Doussineau Tristan , Charles Laurence , Antoine Rodolphe 
Published: December 2015

RationaleCoupling size‐exclusion chromatography (SEC) with mass spectrometry (MS) allowed generation of a SEC calibration curve based on the analyte itself, which is more reliable than calibration based on non‐related standards and faster than the use of the multiple detection mode. However, such SEC/MS couplings were limited to rather small synthetic polymers.MethodsBased on the concept of image current detection, charge‐detection mass spectrometry (CDMS) coupled to electrospray ionization (ESI) is a useful method for weighing macro‐ions from compounds with masses higher than one megadalton (MDa). Using columns designed to allow analysis of synthetic polymers of over 15 million Dalton in mass, performance of the SEC/ESI‐CDMS coupling was evaluated for polyacrylamide (PAM, 5–6 MDa) and a poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) (PAMPS, 2 MDa).ResultsThe SEC/ESI‐CDMS profiles were first compared with SEC‐UV profiles: the systematic shift in retention time was assigned to the slightly different geometries of the two instrumental systems. The SEC/ESI‐CDMS data were then compared with results obtained after the direct infusion of each sample into the ESI source. Both the shape of the molecular weight distribution and the mass values were similar with or without separation prior to ESI, and these values were consistent with data provided by the sample supplier.ConclusionsSEC/MS incorporating an online ESI‐CDMS coupling was shown to be a rapid and efficient technique for the analysis of water‐soluble synthetic polymers with ultra‐high molecular mass in the megadalton range. The coupling also afforded an attractive solution for SEC calibration without the use of external standards. Copyright © 2015 John Wiley & Sons, Ltd.
Morbid Obesity is Associated with Increased Mortality, Surgical Complications, and Incremental Health Care Utilization in the Peri‐Operative Period of Colorectal Cancer Surgery
Authors: Hussan Hisham , Gray Darrell M. , Hinton Alice , Krishna Somashekar G. , Conwell Darwin L. , Stanich Peter P. 
Published: December 2015

AbstractBackgroundMorbid obesity (Basic Mass Index ≥ 40 kg/m2) leads to increased long‐term mortality after colorectal cancer (CRC) surgery. Little is known about its effects on peri‐operative CRC surgery outcomes.Methods85,300 discharges for CRC surgery were identified using the redesigned 2012 National Inpatient Sample. Outcomes of interest were mortality, healthcare charges, and surgical outcomes in morbidly obese patients which were compared to those in nonobese patients.ResultsThere were 4385 (5.14%) morbidly obese patients who underwent CRC surgery during the study period. Morbid obesity was associated with younger age, females, and African Americans in our study (p < 0.05). Morbidly obese patients had higher prevalence of CRC peri‐operative co‐morbidities, surgical complications, and conversions from laparoscopic to open surgery. On multivariate analysis, morbid obesity led to an increased CRC surgery peri‐operative mortality (OR 1.85, 95 % CI 1.15, 2.97). Mortality remained significant even after adjusting for surgical complications (OR 1.79, 95 % CI 1.12, 2.88). Morbidly obese patients undergoing CRC also had a prolonged length of hospitalization (1.22 day, 95 % CI 0.67, 1.78), a $15,582 increase in total hospital charges (95 % CI 8419, 22,745), and increased disposition to short‐term rehabilitation facilities (OR 2.25, 95 % CI 1.79, 2.84).ConclusionAnalysis of national level data demonstrates that morbidly obese patients have an increased CRC surgery peri‐operative mortality with higher prevalence of co‐morbidities, surgical complications, and more health care resource utilization. Future research efforts should concentrate on ameliorating these outcomes in morbidly obese patients.
Safety of Laparoscopic Pelvic Exenteration with Urinary Diversion for Colorectal Malignancies
Authors: Ogura Atsushi , Akiyoshi Takashi , Konishi Tsuyoshi , Fujimoto Yoshiya , Nagayama Satoshi , Fukunaga Yosuke , Ueno Masashi 
Published: December 2015

AbstractBackgroundAlthough the feasibility of laparoscopic surgery for colorectal cancer has been demonstrated, the safety of laparoscopic pelvic exenteration (PE) with urinary diversion for colorectal malignancies remains poorly studied. The present study aimed to examine the safety and feasibility of laparoscopic PE in patients with colorectal malignancies.MethodsThirty‐one consecutive patients who underwent anterior or total PE with urinary diversion for colorectal malignancies between July 2004 and April 2015 at our institution were included in the study. Perioperative outcomes were compared between patients undergoing laparoscopic (n = 13) and open (n = 18) PE.ResultsThere were no conversions to open surgery. The estimated blood loss (930 vs. 3003 ml; P = 0.001) and total volume of blood transfusion (0 vs. 1990 ml; P = 0.002) were significantly lower in patients undergoing laparoscopic compared with open PE. R0 resection was performed in all patients. The operation time (laparoscopic, 829 min vs. open, 875 min; P = 0.660), complication rate (laparoscopic, 61.5 % vs. open, 83.3 %; P = 0.171), and postoperative hospital days (laparoscopic, 29 days vs. open, 33 days; P = 0.356) were similar in both groups. Three patients undergoing laparoscopic PE readmitted within 30 days due to ileus, although the rate of readmission did not differ significantly (laparoscopic, 23.1 % vs. open, 5.6 %; P = 0.284).ConclusionLaparoscopic PE performed by experienced laparoscopic pelvic surgeons should be considered as a safe and preferred option in selected patients with colorectal malignancies, with a significant advantage in terms of reduced blood loss compared with open surgery.
Adenosine kinase deficiency: expanding the clinical spectrum and evaluating therapeutic options
Authors: Staufner Christian , Lindner Martin , Dionisi‐Vici Carlo , Freisinger Peter , Dobbelaere Dries , Douillard Claire , Makhseed Nawal , Straub Beate K. , Kahrizi Kimia , Ballhausen Diana , la Marca Giancarlo , Kölker Stefan , Haas Dorothea , Hoffmann Georg F. , Grünert Sarah C. , Blom Henk J. 
Published: December 2015

AbstractBackgroundAdenosine kinase deficiency is a recently described defect affecting methionine metabolism with a severe clinical phenotype comprising mainly neurological and hepatic impairment and dysmorphism.MethodsClinical data of 11 additional patients from eight families with adenosine kinase deficiency were gathered through a retrospective questionnaire. Two liver biopsies of one patient were systematically evaluated.ResultsThe main clinical symptoms are mild to severe liver dysfunction with neonatal onset, muscular hypotonia, global developmental retardation and dysmorphism (especially frontal bossing). Hepatic involvement is not a constant finding. Most patients have epilepsy and recurrent hypoglycemia due to hyperinsulinism. Major biochemical findings are intermittent hypermethioninemia, increased S‐adenosylmethionine and S‐adenosylhomocysteine in plasma and increased adenosine in urine. S‐adenosylmethionine and S‐adenosylhomocysteine are the most reliable biochemical markers. The major histological finding was pronounced microvesicular hepatic steatosis. Therapeutic trials with a methionine restricted diet indicate a potential beneficial effect on biochemical and clinical parameters in four patients and hyperinsulinism was responsive to diazoxide in two patients.ConclusionAdenosine kinase deficiency is a severe inborn error at the cross‐road of methionine and adenosine metabolism that mainly causes dysmorphism, brain and liver symptoms, but also recurrent hypoglycemia. The clinical phenotype varies from an exclusively neurological to a multi‐organ manifestation. Methionine‐restricted diet should be considered as a therapeutic option.