bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dietary Flavonoid Fisetin Causes Cell Cycle Arrest, Caspase‐Dependent Apoptosis, and Enhanced Cytotoxicity of Chemotherapeutic Drugs in Triple‐Negative Breast Cancer Cells
Authors: Smith Matthew L. , Murphy Kaylee , Doucette Carolyn D. , Greenshields Anna L. , Hoskin David W. 
Published: January 2016

ABSTRACTFisetin (3,3′,4′,7‐tetrahydroxyflavone), a flavonoid found in a number of fruits and vegetables, has diverse biological activities, including cytotoxic effects on cancer cells. In this study, we investigated the effect of fisetin on triple‐negative breast cancer (TNBC) cells. TNBC has a poorer prognosis than other types of breast cancer and treatment options for this disease are limited. Fisetin inhibited the growth of MDA‐MB‐468 and MDA‐MB‐231 TNBC cells, as well as their ability to form colonies, without substantially affecting the growth of non‐malignant cells. In addition, fisetin inhibited the growth of estrogen receptor‐bearing MCF‐7 breast cancer cells and human epidermal growth factor receptor 2‐overexpressing SK‐BR‐3 breast cancer cells. Fisetin inhibited TNBC cell division and induced apoptosis, which was associated with mitochondrial membrane permeabilization and the activation of caspase‐9 and caspase‐8, as well as the cleavage of poly(ADP‐ribose) polymerase‐1. Induction of caspase‐dependent apoptosis by fisetin was confirmed by reduced killing of TNBC cells in the presence of the pan‐caspase inhibitors Z‐VAD‐FMK and BOC‐D‐FMK. Decreased phosphorylation of histone H3 at serine 10 in fisetin‐treated TNBC cells at G2/M phase of the cell cycle suggested that fisetin‐induced apoptosis was the result of Aurora B kinase inhibition. Interestingly, the cytotoxic effect of cisplatin, 5‐fluorouracil, and 4‐hydroxycyclophosphamide metabolite of cyclophosphamide on TNBC cells was increased in the presence of fisetin. These findings suggest that further investigation of fisetin is warranted for possible use in the management of TNBC. J. Cell. Biochem. 117: 1913–1925, 2016. © 2016 Wiley Periodicals, Inc.
Region‐specific localization of NOS isoforms and NADPH‐diaphorase activity in the intratesticular and excurrent duct systems of adult domestic cats (Felis catus)
Authors: Liman Narin , Alan Emel 
Published: January 2016

ABSTRACTNitric oxide (NO) is produced by nitric oxide synthases (NOSs) and plays an important role in all levels of reproduction from the brain to the reproductive organs. Recently, it has been discovered that all germ cells and Leydig cells in the cat testis exhibit stage‐dependent nuclear and cytoplasmic endothelial (eNOS) and inducible (iNOS)‐NOS immunoreactivity and cytoplasmic nicotinamide adenine dinucleotide phosphate‐diaphorase (NADPH‐d) reactivity. As a continuation of this finding, in this study, cellular localization of NADPH‐d and immunolocalization and expression of all three NOS isoforms were investigated in the intratesticular (tubuli recti and rete testis), and excurrent ducts (efferent ductules, epididymal duct and vas deferens) of adult cats using histochemistry, immunohistochemistry and western blotting. NADPH‐d activity was found in the midpiece of the spermatozoa tail and epithelial cells of all of ducts, except for nonciliated cells of the efferent ductules. Even though the immunoblotting results revealed similar levels of nNOS, eNOS and iNOS in the caput, corpus and cauda segments of epididymis and the vas deferens, immunostainings showed cell‐specific localization in the efferent ductules and region‐ and cell‐specific localization in the epididymal duct. All of three NOS isoforms were immunolocalized to the nuclear membrane and cytoplasm of the epithelial cells in all ducts, but were found in the tail and the cytoplasmic droplets of spermatozoa. These data suggest that NO/NOS activity might be of importance not only for the functions of the intratesticular and excurrent ducts but also for sperm maturation. Microsc. Res. Tech. 79:192–208, 2016. © 2016 Wiley Periodicals, Inc.
Fully automatic segmentation of left atrium and pulmonary veins in late gadolinium‐enhanced MRI: Towards objective atrial scar assessment
Authors: Tao Qian , Ipek Esra Gucuk , Shahzad Rahil , Berendsen Floris F. , Nazarian Saman , van der Geest Rob J. 
Published: January 2016

PurposeTo realize objective atrial scar assessment, this study aimed to develop a fully automatic method to segment the left atrium (LA) and pulmonary veins (PV) from late gadolinium‐enhanced (LGE) magnetic resonance imaging (MRI). The extent and distribution of atrial scar, visualized by LGE‐MRI, provides important information for clinical treatment of atrial fibrillation (AF) patients.Materials and MethodsForty‐six AF patients (age 62 ± 8, 14 female) who underwent cardiac MRI prior to RF ablation were included. A contrast‐enhanced MR angiography (MRA) sequence was acquired for anatomy assessment followed by an LGE sequence for LA scar assessment. A fully automatic segmentation method was proposed consisting of two stages: 1) global segmentation by multiatlas registration; and 2) local refinement by 3D level‐set. These automatic segmentation results were compared with manual segmentation.ResultsThe LA and PVs were automatically segmented in all subjects. Compared with manual segmentation, the method yielded a surface‐to‐surface distance of 1.49 ± 0.65 mm in the LA region when using both MRA and LGE, and 1.80 ± 0.93 mm when using LGE alone (P < 0.05). In the PV regions, the distance was 2.13 ± 0.67 mm and 2.46 ± 1.81 mm (P < 0.05), respectively. The difference between automatic and manual segmentation was comparable to the interobserver difference (P = 0.8 in LA region and P = 0.7 in PV region).ConclusionWe developed a fully automatic method for LA and PV segmentation from LGE‐MRI, with comparable performance to a human observer. Inclusion of an MRA sequence further improves the segmentation accuracy. The method leads to automatic generation of a patient‐specific model, and potentially enables objective atrial scar assessment for AF patients. J. Magn. Reson. Imaging 2016;44:346–354.
Perceived organizational support and affective organizational commitment: Moderating influence of perceived organizational competence
Authors: Kim Kyoung Yong , Eisenberger Robert , Baik Kibok 
Published: January 2016

SummaryPerceived organizational support (POS), involving employees' perception that the organization values their contributions and cares about their well‐being, has been found to be the work experience most strongly linked to their emotional bond to the organization (affective organizational commitment, or AC). We suggest that employees' perception concerning the organization's ability to achieve its goals and objectives (perceived organizational competence, or POC) may enhance this relationship by more effectively fulfilling socio‐emotional needs. We conducted three studies with employees in the United States and South Korea to assess the interactive relationship between POS and POC and their distinctive antecedents. Our hierarchical linear modeling and ordinary least squared regression results showed that POC strengthened the relationship between POS and AC and that this association carried over to extra‐role performance. Further, leader initiating structure contributed more to POC than to POS, whereas leader consideration contributed more to POS than to POC. These findings suggest POC plays an important role in moderating the relationship between POS and AC. Copyright © 2016 John Wiley & Sons, Ltd.
Broadband 10 Gb/s operation of graphene electro‐absorption modulator on silicon
Authors: Hu Yingtao , Pantouvaki Marianna , Van Campenhout Joris , Brems Steven , Asselberghs Inge , Huyghebaert Cedric , Absil Philippe , Van Thourhout Dries 
Published: January 2016

AbstractHigh performance integrated optical modulators are highly desired for future optical interconnects. The ultra‐high bandwidth and broadband operation potentially offered by graphene based electro‐absorption modulators has attracted a lot of attention in the photonics community recently. In this work, we theoretically evaluate the true potential of such modulators and illustrate this with experimental results for a silicon integrated graphene optical electro‐absorption modulator capable of broadband 10 Gb/s modulation speed. The measured results agree very well with theoretical predictions. A low insertion loss of 3.8 dB at 1580 nm and a low drive voltage of 2.5 V combined with broadband and athermal operation were obtained for a 50 μm‐length hybrid graphene‐Si device. The peak modulation efficiency of the device is 1.5 dB/V. This robust device is challenging best‐in‐class Si (Ge) modulators for future chip‐level optical interconnects.
Prediction of summer monsoon rainfall over India and its homogeneous regions
Authors: Kakade Santosh , Kulkarni Ashwini 
Published: January 2016

ABSTRACTThe coherent regions for various fields such as sea level pressure, temperature, geopotential height and zonal wind anomalies at the surface, 850, 500 and 200 hPalevels in pre‐monsoon months (January through May) and seasons (winter, spring) have been identified by applying the shared nearest neighbour algorithm. The fields over the corresponding cluster regions could be possible predictors for Indian summer monsoon rainfall as well as the rainfall over various homogeneous regions of India. The time series have been constructed by averaging the parameters over the respective clusters. The relationship between these time series and the summer monsoon rainfall over India and its well‐defined homogeneous regions over India, (northwest India, central northeast India, northeast India, west central India and peninsular India), has been examined during the positive and negative phases of effective strength index tendency using the simple technique of multiple regression. Along with the linear relationship, the non‐linear relationships between the cluster parameters and the seasonal rainfall have also been considered. Independent cluster parameters have been selected by cross‐validation procedure and the performance of each predictive model is tested. The extreme yearly rainfall departures over India are qualitatively well predicted by the model. Also, the unprecedented droughts over India in 2002 and 2009, where all earlier models have failed to forecast, are well predicted by the present model. The performances of models for summer monsoon rainfall prediction over homogeneous regions of India are convincing.
Data mining algorithms to compute mixed concepts with negative attributes: an application to breast cancer data analysis
Authors: Rodríguez‐Jiménez Jose Manuel , Cordero Pablo , Enciso Manuel , Mora Angel 
Published: January 2016

In the design of mathematical methods for a medical problem, one of the kernel issues is the identification of symptoms and measures that could help in the diagnosis. Discovering connections among them constitute a big challenge because it allows to reduce the number of parameters to be considered in the mathematical model. In this work, we focus on formal concept analysis as a very promising technique to address this problem. In previous works, we have studied the use of formal concept analysis to manage attribute implications. In this work, we propose to extend the knowledge that we can extract from every context using positive and negative information, which constitutes an open problem.Based on the main classical algorithms, we propose new methods to generate the lattice concept with positive and negative information to be used as a kind of map of attribute connections. We also compare them in an experiment built with datasets from the UCI repository for machine learning. We finally apply the mining techniques to extract the knowledge contained in a real data set containing information about patients suffering breast cancer. The result obtained have been contrasted with medical scientists to illustrate the benefits of our proposal. Copyright © 2016 John Wiley & Sons, Ltd.
A viscoplastic subloading soil model for rate‐dependent cyclic anisotropic structured behaviour
Authors: Maranha J. R. , Pereira C. , Vieira A. 
Published: January 2016

SummaryThis paper presents a new purely viscoplastic soil model based on the subloading surface concept with a mobile centre of homothety, enabling the occurrence of viscoplastic strains inside the yield surface and avoiding the abrupt change in stiffness of the traditional overstress viscoplastic models. This is required for overconsolidated soils. The model is formulated to reproduce the soil rate‐dependent behaviour under cyclic loading (changes in loading direction) and incorporates both initial and induced anisotropy, as well as destructuring. The model shows good qualitative response to some imposed three‐dimensional stress paths under quasi‐inviscid (elastoplastic) behaviour. Some of the main time‐dependent aspects of soil behaviour that the model is capable of reproducing were also illustrated. The capability of the model to adequately reproduce the results from an undrained triaxial test performed on stiff overconsolidated clays from the Lisbon region (Formação de Benfica), with an unloading–reloading deviatoric stress cycle at constant mean stress, that incorporates a series of staggered fast loading and creep stages, was evaluated. The model was able to reproduce well the main observed aspects of the time‐dependent stress–strain response and pore pressure evolution of a stiff overconsolidated clay under complex loading. The revised and generalised viscoplastic subloading surface concept is viable and can be applied to a consistent extension to viscoplasticity, including in the interior of the yield surface, of existing elastoplastic models formulated for soils and other materials. Copyright © 2016 John Wiley & Sons, Ltd.
Sources, incidence and effects of non‐physical workplace violence against nurses in Ghana
Authors: Boafo Isaac Mensah , Hancock Peter , Gringart Eyal 
Published: January 2016

AbstractAimTo document the incidence, sources and effects of workplace verbal abuse and sexual harassment against Ghanaian nurses.MethodsA cross‐sectional study was conducted in Ghana from 2013–2014 which surveyed 592 professional nurses and midwives working in public hospitals in Ghana using the health sector violence questionnaire.ResultsThe majority of participants were females (80%). The average age of participants was 31·76 years and the average number of years practising as nurse was 7·38. Twelve per cent of the participants experienced at least one incident of sexual harassment and 52·2% were exposed to verbal abuse. The majority of perpetrators of sexual harassment were medical doctors (50%). Relatives of patients emerged as the most frequent verbal abusers (45·5%). Chi‐square test showed statistically significant associations between gender and workplace violence and between workplace violence and intention to quit the nursing profession. The effects of workplace violence ranged from having disturbing memories about the incident to being ‘super alert’ and vigilant.Establishing the incidence of workplace violence is a necessary step towards addressing the problem. It is concluded that educational programs must be designed for healthcare workers and the general public to foster awareness of the effects of workplace violence. Clear policies must also be instituted to address the problem.
Effect of Amide Hydrogen Bonding Interaction on Supramolecular Self‐Assembly of Naphthalene Diimide Amphiphiles with Aggregation Induced Emission
Authors: Ghule Namdev V. , La Duong Duc , Bhosale Rajesh S. , Al Kobaisi Mohammad , Raynor Aaron M. , Bhosale Sheshanath V. , Bhosale Sidhanath V. 
Published: January 2016

AbstractIn the present work, two new naphthalene diimide (NDI) amphiphiles, NDI‐N and NDI‐NA, were successfully synthesized and employed to investigate their self‐assembly and optical properties. For NDI‐NA, which contains an amide group, aggregation‐induced emission enhancement (AIEE) was demonstrated in the presence of various ratios of methylcyclohexane (MCH) in chloroform, which led to the visual color changes. This new amide‐containing NDI‐NA amphiphile formed nanobelt structures in chloroform/MCH (10:90, v/v) and microcup‐like morphologies in chloroform/MCH (5:95, v/v). The closure of these microcups led to the formation of vesicles and microcapsules. The structural morphologies gained from the solvophobic control of NDI‐NA were confirmed by various complementary techniques such as infrared spectroscopy, X‐ray diffraction, and scanning and transmission electron microscopy. In the absence of the amide moiety in NDI‐N, no self‐assembly was observed, indicating the fundamental role of H‐bonding in the self‐association process.
A novel natural rubber‐graft‐cassava starch foam for oil/gasohol absorption
Authors: Riyajan Sa‐Ad , Keawittarit Pramort 
Published: January 2016

AbstractThe objective of this work was to study a novel foam prepared from natural rubber grafted with cassava starch (NR‐graft‐CSt) with the addition of super cell (SC) as a blowing agent. The effects of the blowing agent and grafting versus non‐grafting of the starch and the natural rubber on the properties of the foam were investigated. The results show that the optimum curing time decreases with increasing SC loading as observed using a moving die rheometer. The porosity, the number of cells per unit volume and the cell size of the NR/CSt blend increase as a function of the SC loading in the foam. A more open cell structure is produced by higher loadings of SC. The greatest number of cells per unit volume is found at 2 and 6 phr SC for NR‐graft‐CSt and NR/CSt blend, respectively. The foam produced was used as an absorbent for oil. The NR‐graft‐CSt foam shows a maximum percentage oil absorption of around 7 g g−1. The NR‐graft‐CSt foam is able to be reused as an oil absorbent for a maximum number of over 30 times. The NR‐graft‐CSt foam shows better toluene resistance than the NR/CSt blend. © 2016 Society of Chemical Industry
Seven root canals in a deciduous maxillary molar detected by the dental operating microscope and micro‐computed tomography
Authors: Ahmed Hany Mohamed Aly , Khamis Mohd Fadhli , Gutmann James Leo 
Published: January 2016

SummaryThe root and root canal morphology of deciduous molars shows considerable variations. Consequently, a comprehensive understanding of the normal and unusual root and root canal configuration types in deciduous teeth is of prime importance. The purpose of this report is to describe a rare anatomical variation in a double‐rooted maxillary deciduous molar examined by the dental operating microscope and micro‐computed tomography. SCANNING 38:554–557, 2016. © 2016 Wiley Periodicals, Inc.
Metabolic Reprogramming and Dependencies Associated with Epithelial Cancer Stem Cells Independent of the Epithelial-Mesenchymal Transition Program
Authors: Aguilar Esther , Marin de Mas Igor , Zodda Erika , Marin Silvia , Morrish Fionnuala , Selivanov Vitaly , Meca-Cortés Óscar , Delowar Hossain , Pons Mònica , Izquierdo Inés , Celià-Terrassa Toni , de Atauri Pedro , Centelles Josep J. , Hockenbery David , Thomson Timothy M. , Cascante Marta 
Published: January 2016

Abstract
In solid tumors, cancer stem cells (CSCs) can arise independently of epithelial-mesenchymal transition (EMT). In spite of recent efforts, the metabolic reprogramming associated with CSC phenotypes uncoupled from EMT is poorly understood. Here, by using metabolomic and fluxomic approaches, we identify major metabolic profiles that differentiate metastatic prostate epithelial CSCs (e-CSCs) from non-CSCs expressing a stable EMT. We have found that the e-CSC program in our cellular model is characterized by a high plasticity in energy substrate metabolism, including an enhanced Warburg effect, a greater carbon and energy source flexibility driven by fatty acids and amino acid metabolism and an essential reliance on the proton buffering capacity conferred by glutamine metabolism. An analysis of transcriptomic data yielded a metabolic gene signature for our e-CSCs consistent with the metabolomics and fluxomics analyses that correlated with tumor progression and metastasis in prostate cancer and in 11 additional cancer types. Interestingly, an integrated metabolomics, fluxomics, and transcriptomics analysis allowed us to identify key metabolic players regulated at the post-transcriptional level, suggesting potential biomarkers and therapeutic targets to effectively forestall metastasis.
Histone Chaperone SSRP1 is Essential for Wnt Signaling Pathway Activity During Osteoblast Differentiation
Authors: Hossan Tareq , Nagarajan Sankari , Baumgart Simon J. , Xie Wanhua , Magallanes Roberto Tirado , Hernandez Céline , Chiaroni Pierre-Marie , Indenbirken Daniela , Spitzner Melanie , Thomas-Chollier Morgane , Grade Marian , Thieffry Denis , Grundhoff Adam , Wegwitz Florian , Johnsen Steven A. 
Published: January 2016

Abstract
Cellular differentiation is accompanied by dramatic changes in chromatin structure which direct the activation of lineage-specific transcriptional programs. Structure-specific recognition protein-1 (SSRP1) is a histone chaperone which is important for chromatin-associated processes such as transcription, DNA replication and repair. Since the function of SSRP1 during cell differentiation remains unclear, we investigated its potential role in controlling lineage determination. Depletion of SSRP1 in human mesenchymal stem cells elicited lineage-specific effects by increasing expression of adipocyte-specific genes and decreasing the expression of osteoblast-specific genes. Consistent with a role in controlling lineage specification, transcriptome-wide RNA-sequencing following SSRP1 depletion and the induction of osteoblast differentiation revealed a specific decrease in the expression of genes involved in biological processes related to osteoblast differentiation. Importantly, we observed a specific downregulation of target genes of the canonical Wnt signaling pathway, which was accompanied by decreased nuclear localization of active β-catenin. Together our data uncover a previously unknown role for SSRP1 in promoting the activation of the Wnt signaling pathway activity during cellular differentiation.