bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biofeedback efficacy for outlet dysfunction constipation: Clinical outcomes and predictors of response by a limited approach
Authors: Lambiase Christian , Bellini Massimo , Whitehead William E. , Popa Stefan Lucian , Morganti Riccardo , Chiarioni Giuseppe 
Published: October 2024

AbstractBackgroundFunctional defecation disorders (FDD) are a common etiology of refractory chronic constipation (CC). FDD diagnosis (dyssynergic defecation [DD] and inadequate defecatory propulsion [IDP]), requires diagnostic tests including anorectal manometry (ARM) and balloon expulsion test (BET). Biofeedback (BF) is the treatment of choice for DD. The aims of our study were to evaluate: the outcome of BF in a group of constipated patients with defecatory disorders of any etiology; the efficacy of two simple diagnostic tools in predicting BF outcome in the short‐term.MethodsOne hundred and thirty‐one refractory CC patients failing the BET underwent BF therapy. Before BF, all patients underwent the following:

ARM.
Straining questionnaire. The answers were: “belly muscles”; “anal muscles”; “both”; “Don't know/No answer.”
Digital rectal examination augmented by abdominal palpation on straining (augmented‐DRE).
The BF therapist was blinded to ARM, straining questionnaire, and augmented‐DRE results.Key ResultsEighty‐one patients responded to BF. Gender, age, and IBS‐C showed no significant impact on BF response. Both DD and IDP responded equally to BF, while the rate of response in patients with isolated structural pelvic floor abnormalities was lower (p < 0.001). The answer “anal muscles” to straining questionnaire showed a strong association with BF response (p < 0.001). A lack in abdominal contraction and in anal relaxation on augmented‐DRE were strongly associated with BF response (p < 0.01). Absence of manual maneuvers to facilitate defecation was associated with BF response (p < 0.001).Conclusions & InferencesBF is the therapy of choice for refractory constipation due to FDD of any etiology, inducing both clinical and anorectal physiology improvement in the short term. Comorbid IBS‐C did not affect outcome while symptomatic isolated pelvic floor abnormalities appeared refractory to behavior treatment.The straining questionnaire and augmented‐DRE outcomes showed a strong correlation with BF response and can be implemented in clinical practice to improve the management of constipated patients by prompting early referral to BF.
Ancient large‐scale gene duplications and diversification in bryophytes illuminate the plant terrestrialization
Authors: Shen Chao , Li Hao , Shu Lei , Huang Wen‐Zhuan , Zhu Rui‐Liang 
Published: October 2024

Summary
Large‐scale gene duplications (LSGDs) are crucial for evolutionary adaptation and recurrent in vascular plants. However, the role of ancient LSGDs in the terrestrialization and diversification of bryophytes, the second most species‐rich group of land plants, remains largely elusive due to limited sampling in bryophytes.
Employing the most extensive nuclear gene dataset in bryophytes to date, we reconstructed a time‐calibrated phylogenetic tree from 209 species, covering virtually all key bryophyte lineages, for phylogenomic analyses of LSGDs and diversification.
We newly identified two ancient LSGDs: one in the most recent common ancestor (MRCA) of extant bryophytes and another in the MRCA of the majority of Jungermanniales s. lato. Duplicated genes from these two LSGDs show significant enrichment in photosynthesis‐related processes and structures. Rhizoid‐responsive ROOTHAIR DEFECTIVE SIX‐LIKE (RSL) genes from ancient LSGDs are present in rhizoidless bryophytes, challenging assumptions about rhizoid absence mechanisms. We highlighted four major diversification rate upshifts, two of which slightly postdated LSGDs, potentially linked to the flourishing of gymnosperms and angiosperms and explaining over 80% of bryophyte diversity.
Our findings, supported by extensive bryophyte sampling, highlight the significance of LSGDs in the early terrestrialization and diversification of bryophytes, offering new insights into land plant evolution.
Building labour power in the platform economy: A comparative analysis of worker struggles in German and Norwegian food and grocery delivery
Authors: López Tatiana , Jesnes Kristin , Alyanak Oğuz , Karlıdağ Zeynep 
Published: October 2024

AbstractThis study undertakes a comparative analysis of delivery workers’ struggles in Norway and Germany. Through the theoretical lens of the power resources approach, we analyse how delivery workers in Berlin and Oslo combine associational, structural, institutional, coalitional and discursive power resources, responding to different institutionalised frameworks of industrial relations. We make three contributions to debates on worker agency and resistance in the platform economy. First, we show that delivery workers need to actively combine different power resources to overcome barriers to organising and exercising structural power, resulting from platforms’ model of work organisation. Second, we highlight the role of institutional contexts in shaping workers’ collective organising strategies. Finally, our analysis stresses that the power resources that food delivery workers have built are often contested and circumvented by counter‐practices from the platform companies.
Quantitative Proteomic Analysis of Brassica Napus Reveals Intersections Between Nutrient Deficiency Responses
Authors: Grubb L. E. , Scandola S. , Mehta D. , Khodabocus I. , Uhrig R. G. 
Published: October 2024

ABSTRACTMacronutrients such as nitrogen (N), phosphorus (P), potassium (K) and sulphur (S) are critical for plant growth and development. Field‐grown canola (Brassica napus L.) is supplemented with fertilizers to maximize plant productivity, while deficiency in these nutrients can cause significant yield loss. A holistic understanding of the interplay between these nutrient deficiency responses in a single study and canola cultivar is thus far lacking, hindering efforts to increase the nutrient use efficiency of this important oil seed crop. To address this, we performed a comparative quantitative proteomic analysis of both shoot and root tissue harvested from soil‐grown canola plants experiencing either nitrogen, phosphorus, potassium or sulphur deficiency. Our data provide critically needed insights into the shared and distinct molecular responses to macronutrient deficiencies in canola. Importantly, we find more conserved responses to the four different nutrient deficiencies in canola roots, with more distinct proteome changes in aboveground tissue. Our results establish a foundation for a more comprehensive understanding of the shared and distinct nutrient deficiency response mechanisms of canola plants and pave the way for future breeding efforts.
Guard Cell Activity of PIF4 Represses Disease Resistance in Arabidopsis
Authors: Wang Zhixue , Perez Veronica , Hua Jian 
Published: October 2024

ABSTRACTPhytochrome Interacting Factor 4 (PIF4) plays a central role in coordinating plant growth regulation by integrating multiple environmental cues. However, studies on whether and how PIF4 regulates plant immunity have inconsistent findings. In this study, we investigated the role of PIF4 in disease resistance against Pst DC3000 by characterizing its loss‐of‐function mutants using different inoculation strategies. Our findings reveal that pif4 mutants exhibit enhanced disease resistance with spray inoculation but not with infiltration inoculation compared to wild‐type plants, and that mutants displayed more closed stomata apertures, indicating that PIF4 promotes stomatal opening. Importantly, expression of PIF4 by a guard‐cell‐specific promoter was sufficient to restore disease resistance to the wild‐type level in the pif4 mutant. Additionally, PIF4 overexpression enhances disease symptom development independent of disease resistance and chlorophyll degradation, while the loss of PIF4 function leads to higher chlorophyll accumulation. Thus, our findings highlight a crucial function of PIF4 in regulating stomata‐mediated disease resistance and chlorophyll accumulation, providing new insights into the connection of growth and defense in plants.
Philosophies of being in India I: Pluralism, nihilism, and monism
Authors: Frazier Jessica Michelle 
Published: October 2024

AbstractIs Being a mere sum of separate things variously re‐combined over time? Or is it not there at all, arising from nothing more than the projection of a fevered metaphysical imagination? Or might it be the intrawoven phenomenon of all we experience, grounded in a single underlying all‐determining nature? This is the first of a pair of articles on the ontological issues that prompted a philosophical fork between pluralism, nihilism, and monism in India. The present article will focus on Indian critiques of metaphysical pluralism based on the apparent incoherence of ultimately individuating reality's building blocks. The second article will focus on debates about grounding of the causal powers that shape the world and fix it modally. I trace critiques of pluralism (the view found in Vaiśeṣika and Abhidharma that Being is composed of plural fundamental ‘bits’) through to the competing conclusions of the Madhyamaka ‘emptiness’ view, and Vedānta's idea that there is a single ground of all Being. For the nihilists, commonsense realism about Being must be repudiated in such a way as to destroy the notion of Being itself. For the monists, the philosophical problems with a pluralistic ontology show that Being is a complex yet holistic medium, and the ground of a cosmos‐defining modal inheritance. Thus in each article I tell the story of how debates about individuation, relation, causation, recombination and modal inheritance blossomed into some of history's most radical philosophies of Being—and in each I set out some prospects for the monistic view's core ontological claims that Being is, and is one.
The influence of aridity on plant intraspecific chemical diversity supports adaptive differentiation and convergent evolution
Authors: Castells E. , Sanchez‐Martinez P. 
Published: October 2024

Abstract
Plants synthesize a broad array of specialized chemical compounds that mediate their interactions with the surrounding environment. Some of this chemical diversity is functional and subject to natural selection, but the factors underlying chemical evolution at the intraspecific level remain largely unknown. Here, we combined chemical, environmental and genetic data to investigate the effect of aridity on the expression of chemotypes in the invasive shrub Senecio pterophorus.
We studied the variation in pyrrolizidine alkaloids (PAs), a group of specialized metabolites widespread across the families Boraginaceae, Asteraceae and Fabaceae, from native populations spanning a cline of aridity and from three cross‐continental introductions, under natural and common garden conditions. We examined whether the relationship between chemistry and aridity was compatible with a process of adaptive differentiation using a method that partitions the variance and covariance by controlling for the population neutral genetic structure.
We found a consistent shift from retrorsine‐like to seneciphylline‐like compounds under increasing aridity in both natural and controlled conditions in coherence with the biosynthetic pathways. This pattern was independent of the neutral genetic structure and occurred along the environmental gradient in the native range and in a convergent manner in all nonnative regions, which suggests adaptive differentiation in response to aridity.
Our findings show that the diversity of PAs in S. pterophorus has been partially shaped by aridity. Investigating how abiotic factors influence chemical evolution is key to elucidating the plant responses in future climate scenarios and the cascading effects on other trophic levels.
From Asia to Iberia: the mobility of early modern reliquaries☆
Authors: Maratsos Jessica 
Published: October 2024

AbstractIn his famous 1543 Treatise on Relics, Jean Calvin complained that if one were to gather all the purported pieces of the True Cross from the churches and cathedrals of Europe, it would ‘form a whole ship's cargo’. This comment not only highlights the questionable authenticity of relics, but also alludes to their inherent mobility. Indeed, as portable religious objects, relics played an essential role in the drive to export Christianity across the globe. Bound as it was to contemporary mercantile and colonial politics, Christian proselytization was imbricated with the circulation of material goods. Objects crafted in far off regions, such as Gujarat and Nagasaki, gained new life in European liturgical contexts. This essay examines imported caskets crafted from mother‐of‐pearl, tortoiseshell, and lacquer that were transformed into reliquaries on the Iberian Peninsula arguing that such reliquaries harnessed the established potency of the itinerant relic, while simultaneously inventing a new visual idiom that spoke to the precarity of the Catholic faith as it traversed great transoceanic distances. In so doing, it demonstrates the active role played by technologies of transit in the shaping of material and devotional culture in the early modern era.
A concealable intervention for a concealed group: Applying self‐affirmation interventions to people with concealable stigmatized identities
Authors: Monheim Chelsea L. , Himmelstein Mary S. 
Published: October 2024

AbstractPeople with concealable stigmatized identities (CSIs)—identities that are devalued by society, but membership can be hidden (e.g., sexual or gender minority, mental illness, sexual assault survivor)—report high rates of negative health outcomes (e.g., depression, anxiety, illness symptoms). The current literature on CSIs suggests that disclosing one's CSI may promote positive health outcomes, but this approach leaves behind individuals who are unable to disclose due to financial, safety, or personal reasons. The current integrative review examines how self‐affirmation –reaffirming a part of one's identity after a psychologically threatening experience—may buffer against negative health outcomes among people with CSIs; a technique that does not require disclosure to reap the benefits. The purpose of this article is to describe why self‐affirmation may be effective at targeting various factors of CSIs (e.g., anticipated stigma, internalization, centrality, salience) and to call for interventions examining self‐affirmations in this population.
Boron confers salt tolerance through facilitating BnaA2.HKT1‐mediated root xylem Na+ unloading in rapeseed (Brassica napus L.)
Authors: Hua Yingpeng , Pei Minnan , Song Haili , Liu Ying , Zhou Ting , Chao Hongbo , Yue Caipeng , Huang Jinyong , Qin Guangyong , Feng Yingna 
Published: October 2024

SUMMARYBoron (B) is an important limiting factor for plant growth and yield in saline soils, but the underlying molecular mechanisms remain poorly understood. In this study, we found that appropriate B supply obviously complemented rapeseed (Brassica napus L.) growth under salinity accompanied by higher biomass production and less reactive oxygen species accumulation. Determination of Na+ content in shoots and roots indicated that B significantly repressed root‐to‐shoot Na+ translocation, and non‐invasive micro‐tests of root xylem sap demonstrated that B increased xylem Na+ unloading in the roots of rapeseed plants under salinity. Comparative transcriptomic profiling revealed that B strongly upregulated BnaHKT1s expression, especially BnaA2.HKT1, in rapeseed roots exposed to salinity. In situ hybridizations analysis showed that BnaA2.HKT1 was significantly induced in root stelar tissues by high B (HB) under salinity. Green fluorescent protein and yeast heterologous expression showed that BnaA2.HKT1 functioned as a plasma membrane‐localized Na+ transporter. Knockout of BnaA2.HKT1 by CRISPR/Cas9 resulted in hypersensitive of rapeseed plants to salinity even under HB condition, with higher shoot Na+ accumulation and lower biomass production. By contrast, overexpression of BnaA2.HKT1 ameliorated salinity‐induced growth inhibition under B deficiency and salinity. Overall, our results proposed that B functioned as a positive regulator for the rapeseed growth and seed production under salt stress through facilitating BnaA2.HKT1‐mediated root xylem Na+ unloading. This study may also provide an alternative strategy for the improvement of crop growth and development in saline soils.
Expanding the BonnMu sequence‐indexed repository of transposon induced maize (Zea mays L.) mutations in dent and flint germplasm
Authors: Win Yan Naing , Stöcker Tyll , Du Xuelian , Brox Alexa , Pitz Marion , Klaus Alina , Piepho Hans‐Peter , Schoof Heiko , Hochholdinger Frank , Marcon Caroline 
Published: October 2024

SUMMARYThe BonnMu resource is a transposon tagged mutant collection designed for functional genomics studies in maize. To expand this resource, we crossed an active Mutator (Mu) stock with dent (B73, Co125) and flint (DK105, EP1, and F7) germplasm, resulting in the generation of 8064 mutagenized BonnMu F2‐families. Sequencing of these Mu‐tagged families revealed 425 924 presumptive heritable Mu insertions affecting 36 612 (83%) of the 44 303 high‐confidence gene models of maize (B73v5). On average, we observed 12 Mu insertions per gene (425 924 total insertions/36 612 affected genes) and 53 insertions per BonnMu F2‐family (425 924 total insertions/8064 families). Mu insertions and photos of seedling phenotypes from segregating BonnMu F2‐families can be accessed through the Maize Genetics and Genomics Database (MaizeGDB). Downstream examination via the automated Mutant‐seq Workflow Utility (MuWU) identified 94% of the presumptive germinal insertion sites in genic regions and only a small fraction of 6% inserting in non‐coding intergenic sequences of the genome. Consistently, Mu insertions aligned with gene‐dense chromosomal arms. In total, 42% of all BonnMu insertions were located in the 5′ untranslated region of genes, corresponding to accessible chromatin. Furthermore, for 38% of the insertions (163 843 of 425 924 total insertions) Mu1, Mu8 and MuDR were confirmed to be the causal Mu elements. Our publicly accessible European BonnMu resource has archived insertions covering two major germplasm groups, thus facilitating both forward and reverse genetics studies.
Insights into physiological roles of flavonoids in plant cold acclimation
Authors: Kitashova Anastasia , Lehmann Martin , Schwenkert Serena , Münch Maximilian , Leister Dario , Nägele Thomas 
Published: October 2024

SUMMARYFlavonoids represent a diverse group of plant specialised metabolites which are also discussed in the context of dietary health and inflammatory response. Numerous studies have revealed that flavonoids play a central role in plant acclimation to abiotic factors like low temperature or high light, but their structural and functional diversity frequently prevents a detailed mechanistic understanding. Further complexity in analysing flavonoid metabolism arises from the different subcellular compartments which are involved in biosynthesis and storage. In the present study, non‐aqueous fractionation of Arabidopsis leaf tissue was combined with metabolomics and proteomics analysis to reveal the effects of flavonoid deficiencies on subcellular metabolism during cold acclimation. During the first 3 days of a 2‐week cold acclimation period, flavonoid deficiency was observed to affect pyruvate, citrate and glutamate metabolism which indicated a role in stabilising C/N metabolism and photosynthesis. Also, tetrahydrofolate metabolism was found to be affected, which had significant effects on the proteome of the photorespiratory pathway. In the late stage of cold acclimation, flavonoid deficiency was found to affect protein stability, folding and proteasomal degradation, which resulted in a significant decrease in total protein amounts in both mutants. In summary, these findings suggest that flavonoid metabolism plays different roles in the early and late stages of plant cold acclimation and significantly contributes to establishing a new protein homeostasis in a changing environment.
Whole‐chromosome oligo‐painting in licorice unveils interspecific chromosomal evolutionary relationships and possible origin of triploid genome species
Authors: Meng Zhuang , Zheng Qian , Shi Shandang , Wang Wei , Wang Fei , Xie Quanliang , Chen Xifeng , Shen Haitao , Xiao Guanghui , Li Hongbin 
Published: October 2024

SUMMARYLicorice is one of the most extensively studied medicinal plants in the world, whose roots and rhizomes have long been used as both a sweetener and an essential component in numerous herbal preparations. However, the genus Glycyrrhiza has a complex composition, and the interspecies chromosomal relationships, origin, and evolution are still largely unclear. Here, we develop a set of whole‐chromosome painting probes that allowed identification of all eight chromosomes of licorice on same metaphase chromosomes. Comparative chromosome painting analyses in seven different Glycyrrhiza species revealed that the genus Glycyrrhiza maintained extraordinarily conserved chromosomal synteny after about 3–12 million years of divergence. No cytologically visible inter‐chromosomal rearrangements were identified in any species. By comparative chromosomal karyotype analyses, we revealed interspecific chromosome evolutionary relationships and dramatic variable chromosomal karyotype after independent divergence and demonstrated that G. prostrate was the most closely related to the ancestral type among the seven Glycyrrhiza species. Furthermore, we also discovered a G. glandulosa seed with distinct triploid‐genome for the first time in China, suggesting the existence of a polyploid evolutionary pathway in the genus Glycyrrhiza, which challenges the previous notion that only diploids of licorice existed in nature. This study expands our knowledge of the chromosome evolution of licorice and will lay an important foundation for the genome origin and evolution studies in the genus Glycyrrhiza.
Advancing Vehicle Trajectory Prediction: A Probabilistic Approach Using Combined Sequential Models
Authors: Ren Lichuan , Xi Zhimin 
Published: October 2024

Abstract
This paper addresses the critical need to quantify vehicle trajectory uncertainty in autonomous driving under environmental variability. We focus on predicting the posterior distribution of vehicle trajectories over a fixed horizon, given an initial state and a sequence of actions. We propose and compare three approaches: a probabilistic seq2seq model based on stochastic variational Gaussian processes, sequential Monte Carlo simulation with a single-step Gaussian process model, and a hybrid model that leverages the strengths of both methods. Each approach incorporates a baseline vehicle kinematics model to enhance stability and convergence. We evaluate these methods using a dataset generated from the CARLA simulator, assessing both point error metrics and probabilistic prediction metrics. This research introduces novel approaches to quantifying vehicle trajectory uncertainty through various uncertainty quantification techniques, with the goal of improving the safety and reliability of autonomous vehicle control systems.