bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sun protective behaviors at an outdoor entertainment event in Australia
Authors: Barrett Fiona , Usher Kim , Woods Cindy , Harrison Simone L. , Nikles Jane , Conway Jane 
Published: December 2017

AbstractAn observational study was conducted to examine the use of sun protective hats, clothing, and sunglasses of people attending an outdoor entertainment event in an area of high‐to‐extreme ultraviolet radiation in New South Wales, Australia. Armidale is unique, as it is a highly‐elevated area, almost 1000 m above sea level, and temperatures are often mild with very high‐to‐extreme levels of ultraviolet radiation. Four trained data collectors observed attendees as they entered the event, and recorded their use of sun protective hats, clothing, and sunglasses. While more than half of the attendees wore sun protective hats, only 14% wore sun protective clothing. Broad‐brimmed hats were considered sun protective, while sun protective clothing was defined by shirts with at least three‐quarter‐length sleeves. Females were more likely to wear both a sun protective hat and clothing than males, and children were less protected than adults. Legislative changes are required to ensure that organizers of outdoor events have a legal responsibility to provide a safe environment for attendees, including strategies to help reduce ultraviolet radiation exposure.
Redesigning the patient classification system in China: A pilot study
Authors: Bai Yamei , Yao Aaron , Song Yulei , Xu Guihua 
Published: December 2017

AbstractThe current prototype patient classification system in China has not been updated for over six decades. In the present study, we adopted a hybrid patient classification method using both disease severity and activities of daily living scores to classify patients. The time motion approach was used to measure the direct nursing time of 551 general acute care patients. We found that patients in old Categories according to ability of self‐care and disease severity 1–4 received approximately 7.1, 4.6, 3.4, and 4.5 h of direct nursing care, and the number of hours was not significantly different between Categories 2, 3, and 4. In contrast, patients in new Categories 1–4 received approximately 10.1, 6.9, 4.4, and 2.4 h of direct nursing care in 24 h. The nursing hours were significantly different between all pairings of the new categories. The new classification system can be used to make nursing care assignments and adjust staffing.
Estimation of jaw‐opening forces in adults
Authors: Brunton P. A. , Loch C. , Waddell J. N. , Bodansky H. J. , Hall R. , Gray A. 
Published: December 2017

Structured AbstractObjectivesTo estimate maximum jaw‐opening forces in healthy participants of diverse ancestry and to estimate whether opening forces are associated with sex, age and anthropometric parameters such as height, weight and BMI.Setting and Sample PopulationOne hundred and forty‐nine participants aged 20‐60 years with overall good oral and general health. Exclusion criteria included myofascial or neck pain, symptomatic temporomandibular joint disorders (TMD), current orthodontic treatment or the absence of a natural dentition.Material and MethodsJaw‐opening forces were measured with an adjustable rigid extra‐oral device connected to a 1000 N load cell. Seven attempts were recorded, with 10 seconds interval. Median force values were obtained after discarding the first and last attempt. The height and weight of each participant were measured and recorded, alongside age, sex and ethnicity.ResultsMen had greater maximum opening force median values than women (P < .001). Median (IQR) values for women were 41.16 N (30.44) and 79.00 N for men (63.86). Jaw‐opening force values were poorly associated with biological and anthropometric parameters.ConclusionIn this study, which included a large sample of participants of broad age range and from a demographically diverse background, jaw‐opening force values were greater in males than in females; however, force values were poorly associated with biological and anthropometric parameters. Future studies should explore the potential of this method as a screening tool for TMJ disorders and other conditions.
Extracellular matrix influence in Streptococcus mutans gene expression in a cariogenic biofilm
Authors: Florez Salamanca E.J. , Klein M.I. 
Published: December 2017

Caries etiology is biofilm–diet‐dependent. Biofilms are highly dynamic and structured microbial communities enmeshed in a three‐dimensional extracellular matrix. The study evaluated the expression dynamics of Streptococcus mutans genes associated with exopolysaccharides (EPS) (gtfBCD, gbpB, dexA), lipoteichoic acids (LTA) (dltABCD, SMU_775c) and extracellular DNA (eDNA) (lytST, lrgAB, ccpA) during matrix development within a mixed‐species biofilm of S. mutans, Actinomyces naeslundii and Streptococcus gordonii. Mixed‐species biofilms using S. mutans strains UA159 or ΔgtfB formed on saliva‐coated hydroxyapatite discs were submitted to a nutritional challenge (providing an abundance of sucrose and starch). Biofilms were removed at eight developmental stages for gene expression analysis by quantitative polymerase chain reaction. The pH of spent culture media remained acidic throughout the experimental periods, being lower after sucrose and starch exposure. All genes were expressed at all biofilm developmental phases. EPS‐ and LTA‐associated genes had a similar expression profile for both biofilms, presenting lower levels of expression at 67, 91 and 115 hours and a peak of expression at 55 hours, but having distinct expression magnitudes, with lower values for ΔgtfB (eg, fold‐difference of ~382 for gtfC and ~16 for dltB at 43 hours). The eDNA‐associated genes presented different dynamics of expression between both strains. In UA159 biofilms lrgA and lrgB genes were highly expressed at 29 hours (which were ~13 and ~5.4 times vs ΔgtfB, respectively), whereas in ΔgtfB biofilms an inverse relationship between lytS and lrgA and lrgB expression was detected. Therefore, the deletion of gtfB influences dynamics and magnitude of expression of genes associated with matrix main components.
Radiation Dose Exposure for Lumbar Transforaminal Epidural Steroid Injections and Facet Joint Blocks Under CT vs. Fluoroscopic Guidance
Authors: Maino Paolo , Presilla Stefano , Colli Franzone Paola A. , van Kuijk Sander M.J. , Perez Roberto S.G.M. , Koetsier Eva 
Published: December 2017

AbstractBackgroundTransforaminal epidural steroid injections (TFESIs) and facet joint blocks can be performed under fluoroscopy or computed tomography (CT) guidance. The purpose of this retrospective cohort study was to compare patient radiation dose for lumbar TFESIs and facet joint blocks under CT guidance vs. fluoroscopic guidance.MethodsThe primary outcome of this retrospective cohort study was the difference between the estimated effective dose (ED) of CT guidance and fluoroscopic guidance for TFESIs and facet joint blocks. Patients who had undergone these procedures with both CT and fluoroscopic guidance were eligible for this study. Dose‐length product for CT‐guided procedures and dose‐area product for fluoroscopic‐guided procedures were retrospectively collected and converted to ED. Within‐ or between‐group comparisons were performed with appropriate nonparametric tests, using a P value of < 0.05 to indicate statistical significance.ResultsThe 42 patients included in this study underwent a total of 100 procedures. The median estimated ED differed significantly between CT‐guided injections and fluoroscopic‐guided injections (1.59 mSv (interquartile range [IQR] 0.78 to 3.09) vs. 0.19 mSv (IQR 0.11 to 0.30) (Wilcoxon signed rank test, P < 0.001).ConclusionsThe study results suggest that TFESIs and facet joint blocks performed with CT guidance are associated with more than 8 times higher patient radiation dose exposure compared to fluoroscopic guidance. There needs to be more vigilance with regards to CT guidance in interventional pain procedures.
Epidemiology of ocular disorders presumed to be inherited in three small Italian dog breeds in Italy
Authors: Guandalini Adolfo , Di Girolamo Nicola , Corvi Roberta , Santillo Daniele , Andreani Valentina , Pinzo Barbara 
Published: December 2017

AbstractObjectiveTo describe the prevalence and the types of eye disorders that are known or presumed to be inherited (KP‐HED) in three small Italian dog breeds.AnimalsThree small Italian dog breeds: Maltese, Bolognese, and Italian Greyhound.ProceduresAll dogs of the breeds selected for this prospective observational study that underwent a complete ophthalmic examination between 1994 and 2015 were included. General and proportional KP‐HED prevalence with 95% confidence intervals were reported.ResultsThree hundred and six of 462 dogs were affected by at least one KP‐HED (66.2%; 95% CI: 61.8%–70.4%). In the entire population, the five most common KP‐HED were cataract (n = 122; rate on the total number of KP‐HED: 31.4%), entropion (n = 56; 14.4%), keratoconjunctivitis sicca (n = 33; 8.5%), retinal dysplasia (n = 24; 6.2%), and persistent pupillary membrane (iris to iris) (n = 21; 5.4%). The most common KP‐HED in each breed were cataracts in the Maltese (35.1%) and in the Bolognese (24.2%), and presentation of vitreous in the anterior chamber in the Italian Greyhound (46.7%).ConclusionsClinicians should be aware of KP‐HED that commonly affect three small Italian dog breeds. Breed standards should be reconsidered, and breeding programs should be directed at limiting such disorders.
Core Temperature Responses to Cold-Water Immersion Recovery: A Pooled-Data Analysis
Authors: Stephens Jessica M. , Sharpe Ken , Gore Christopher , Miller Joanna , Slater Gary J. , Versey Nathan , Peiffer Jeremiah , Duffield Rob , Minett Geoffrey M. , Crampton David , Dunne Alan , Askew Christopher D. , Halson Shona L. 
Published: December 2017

Purpose:To examine the effect of postexercise cold-water immersion (CWI) protocols, compared with control (CON), on the magnitude and time course of core temperature (Tc) responses.Methods:Pooled-data analyses were used to examine theTcresponses of 157 subjects from previous postexercise CWI trials in the authors’ laboratories. CWI protocols varied with different combinations of temperature, duration, immersion depth, and mode (continuous vs intermittent).Tcwas examined as a double difference (ΔΔTc), calculated as the change inTcin CWI condition minus the corresponding change in CON. The effect of CWI on ΔΔTcwas assessed using separate linear mixed models across 2 time components (component 1, immersion; component 2, postintervention).Results:Intermittent CWI resulted in a mean decrease in ΔΔTcthat was 0.25°C (0.10°C) (estimate [SE]) greater than continuous CWI during the immersion component (P = .02). There was a significant effect of CWI temperature during the immersion component (P = .05), where reductions in water temperature of 1°C resulted in decreases in ΔΔTcof 0.03°C (0.01°C). Similarly, the effect of CWI duration was significant during the immersion component (P = .01), where every 1 min of immersion resulted in a decrease in ΔΔTcof 0.02°C (0.01°C). The peak difference inTcbetween the CWI and CON interventions during the postimmersion component occurred at 60 min postintervention.Conclusions:Variations in CWI mode, duration, and temperature may have a significant effect on the extent of change inTc. Careful consideration should be given to determine the optimal amount of core cooling before deciding which combination of protocol factors to prescribe.
Femoral Neck Stress in Older Adults During Stair Ascent and Descent
Authors: Deng Chen , Gillette Jason C. , Derrick Timothy R. 
Published: December 2017

A detailed understanding of the hip loading environment is needed to help prevent hip fractures, minimize hip pain, rehabilitate hip injuries, and design osteogenic exercises for the hip. The purpose of this study was to compare femoral neck stress during stair ascent and descent and to identify the contribution of muscles and reaction forces to the stress environment in mature adult subjects (n = 17; age: 50–65 y). Motion analysis and inverse dynamics were combined with musculoskeletal modeling and optimization, then used as input to an elliptical femoral neck cross-sectional model to estimate femoral neck stress. Peak stress values at the 2 peaks of the bimodal stress curves (stress vs time plot) were compared between stair ascent and descent. Stair ascent had greater compressive stress than descent during the first peak at the anterior (ascent: −18.0 [7.9] MPa, descent: −12.9 [5.4] MPa, P < .001) and posterior (ascent: −34.4 [10.9] MPa, descent: −27.8 [10.1] MPa, P < .001) aspects of the femoral neck cross section. Stair descent had greater tensile stress during both peaks at the superior aspect (ascent: 1.3 [7.0] MPa, descent: 24.8 [9.7] MPa, peak 1: P < .001; ascent: 15.7 [6.1] MPa, descent: 18.0 [8.4] MPa, peak 2: P = .03) and greater compressive stress during the second peak at the inferior aspect (ascent: −43.8 [9.7] MPa, descent: −51.1 [14.3] MPa, P = .004). Understanding this information can provide a more comprehensive view of bone loading at the femoral neck for older population.
Cohort profile: Study of Transition, Outcomes and Gender (STRONG) to assess health status of transgender people
Authors: Quinn Virginia P , Nash Rebecca , Hunkeler Enid , Contreras Richard , Cromwell Lee , Becerra-Culqui Tracy A , Getahun Darios , Giammattei Shawn , Lash Timothy L , Millman Andrea , Robinson Brandi , Roblin Douglas , Silverberg Michael J , Slovis Jennifer , Tangpricha Vin , Tolsma Dennis , Valentine Cadence , Ward Kevin , Winter Savannah , Goodman Michael 
Published: December 2017

PurposeThe Study of Transition, Outcomes and Gender (STRONG) was initiated to assess the health status of transgender people in general and following gender-affirming treatments at Kaiser Permanente health plans in Georgia, Northern California and Southern California. The objectives of this communication are to describe methods of cohort ascertainment and data collection and to characterise the study population.ParticipantsA stepwise methodology involving computerised searches of electronic medical records and free-text validation of eligibility and gender identity was used to identify a cohort of 6456 members with first evidence of transgender status (index date) between 2006 and 2014. The cohort included 3475 (54%) transfeminine (TF), 2892 (45%) transmasculine (TM) and 89 (1%) members whose natal sex and gender identity remained undetermined from the records. The cohort was matched to 127 608 enrollees with no transgender evidence (63 825 women and 63 783 men) on year of birth, race/ethnicity, study site and membership year of the index date. Cohort follow-up extends through the end of 2016.Findings to dateAbout 58% of TF and 52% of TM cohort members received hormonal therapy at Kaiser Permanente. Chest surgery was more common among TM participants (12% vs 0.3%). The proportions of transgender participants who underwent genital reconstruction surgeries were similar (4%–5%) in the two transgender groups. Results indicate that there are sufficient numbers of events in the TF and TM cohorts to further examine mental health status, cardiovascular events, diabetes, HIV and most common cancers.Future plansSTRONG is well positioned to fill existing knowledge gaps through comparisons of transgender and reference populations and through analyses of health status before and after gender affirmation treatment. Analyses will include incidence of cardiovascular disease, mental health, HIV and diabetes, as well as changes in laboratory-based endpoints (eg, polycythemia and bone density), overall and in relation to gender affirmation therapy.
Cross-sectional analyses of participation in cancer screening and use of hormone replacement therapy and medications in meat eaters and vegetarians: the EPIC-Oxford study
Authors: Tong Tammy Y N , Appleby Paul N , Bradbury Kathryn E , Key Timothy J 
Published: December 2017

ObjectivesTo examine differences in health-related behaviours such as screening or testing for cancer, use of hormone replacement therapy (HRT) and use of other medications in different diet groups.DesignWe studied 31 260 participants across four diet groups (18 155 meat eaters, 5012 fish eaters, 7179 vegetarians, 914 vegans) in the UK EPIC-Oxford cohort. Information was collected in 5-year (around 2000–2003) or 10-year (around 2007) follow-up questionnaires regarding participation in breast screening, cervical screening, prostate-specific antigen (PSA) testing, use of HRT and use of medications for the past 4 weeks. Using Poisson regression, we estimated the prevalence ratios (PR) for each behaviour across people of different diet groups, using meat eaters as the reference group.ResultsCompared with meat eaters, vegetarian (PR: 0.94, 95% CI 0.89 to 0.98) and vegan (PR: 0.82, 95% CI 0.71 to 0.95) women reported lower participation in breast screening, and vegetarian men were less likely to report PSA testing (PR: 0.82, 95% CI 0.71 to 0.96). No differences were observed among women for cervical screening. In women, all non-meat-eating groups reported lower use of HRT compared with meat eaters (P heterogeneity <0.0001). Lower reported use of any medication was observed for participants in all non-meat-eating groups with no (P<0.0001) or one (P=0.0002) self-reported illness. No heterogeneity was observed across the diet groups for the reported use of specific medication for high blood pressure, high blood cholesterol, asthma, diabetes and thyroid disease.ConclusionsDifferences in self-reported breast screening, PSA testing, HRT use and overall medication use were observed across the diet groups. Whether such differences contribute to differential long-term disease risks requires further study.
Comprehensive overview of computer-based health information tailoring: a scoping review protocol
Authors: Ghalibaf Azadeh Kamel , Nazari Elham , Gholian-Aval Mahdi , Tabesh Hamed , Tara Mahmood 
Published: December 2017

IntroductionTailoring health information to the needs of individuals has become an important part of modern health communications. Tailoring has been addressed by researchers from different disciplines leading to the emergence of a wide range of approaches, making the newcomers confused. In order to address this, a comprehensive overview of the field with the indications of research gaps, tendencies and trends will be helpful. As a result, a systematic protocol was outlined to conduct a scoping review within the field of computer-based health information tailoring.Methods and analysisThis protocol is based on the York’s five-stage framework outlined by Arksey and O’Malley. A field-specific structure was defined as a basis for undertaking each stage. The structure comprised three main aspects:system design,information communicationandevaluation. Five leading databases were searched: PubMed, Scopus, Science Direct, EBSCO and IEEE and a broad search strategy was used with less strict inclusion criteria to cover the breadth of evidence. Theoretical frameworks were used to develop the data extraction form and a rigorous approach was introduced to identify the categories from data. Several explanatory-descriptive methods were considered to analyse the data, from which some were proposed to be employed for the first time in scoping studies.Ethics and disseminationThis study investigates the breadth and depth of existing literature on computer-tailoring and as a secondary analysis, does not require ethics approval. We anticipate that the results will identify research gaps and novel ideas for future studies and provide direction to combine methods from different disciplines. The research findings will be submitted for publication to relevant peer-reviewed journals and conferences targeting health promotion and patient education.
Familial and Somatic BAP1 Mutations Inactivate ASXL1/2-Mediated Allosteric Regulation of BAP1 Deubiquitinase by Targeting Multiple Independent Domains
Authors: Peng Hongzhuang , Prokop Jeremy , Karar Jayashree , Park Kyewon , Cao Li , Harbour J. William , Bowcock Anne M. , Malkowicz S. Bruce , Cheung Mitchell , Testa Joseph R. , Rauscher Frank J. 
Published: December 2017

Abstract
Deleterious mutations of the ubiquitin carboxy-terminal hydrolase BAP1 found in cancers are predicted to encode inactive truncated proteins, suggesting that loss of enzyme function is a primary tumorigenic mechanism. However, many tumors exhibit missense mutations or in-frame deletions or insertions, often outside the functionally critical UCH domain in this tumor suppressor protein. Thus, precisely how these mutations inactivate BAP1 is unknown. Here, we show how these mutations affect BAP1 interactions with the Polycomb group-like protein, ASXL2, using combinations of computational modeling technology, molecular biology, and in vitro reconstitution biochemistry. We found that the BAP1–ASXL2 interaction is direct and high affinity, occurring through the ASXH domain of ASXL2, an obligate partner for BAP1 enzymatic activity. The ASXH domain was the minimal domain for binding the BAP1 ULD domain, and mutations on the surfaces of predicted helices of ASXH abolished BAP1 association and stimulation of BAP1 enzymatic activity. The BAP1-UCH, BAP1-ULD, and ASXH domains formed a cooperative stable ternary complex required for deubiquitination. We defined four classes of alterations in BAP1 outside the UCH domain, each failing to productively recruit ASXH to the wild-type BAP1 catalytic site via the ULD, resulting in loss of BAP1 ubiquitin hydrolase activity. Our results indicate that many BAP1 mutations act allosterically to inhibit ASXH binding, thereby leading to loss of enzyme activity. Small-molecule approaches to reactivate latent wild-type UCH activity of these mutants might be therapeutically viable.
Significance: Combined computational and biochemical approaches demonstrate that the BAP1–ASXL2 interaction is direct and high affinity and that many BAP1 mutations act allosterically to inhibit BAP1–ASXL2 binding. Cancer Res; 78(5); 1200–13. ©2017 AACR.
An Anti-GDNF Family Receptor Alpha 1 (GFRA1) Antibody–Drug Conjugate for the Treatment of Hormone Receptor–Positive Breast Cancer
Authors: Bhakta Sunil , Crocker Lisa M. , Chen Yvonne , Hazen Meredith , Schutten Melissa M. , Li Dongwei , Kuijl Coenraad , Ohri Rachana , Zhong Fiona , Poon Kirsten A. , Go Mary Ann T. , Cheng Eric , Piskol Robert , Firestein Ron , Fourie-O'Donohue Aimee , Kozak Katherine R. , Raab Helga , Hongo Jo-Anne , Sampath Deepak , Dennis Mark S. , Scheller Richard H. , Polakis Paul , Junutula Jagath R. 
Published: December 2017

Abstract
Luminal A (hormone receptor-positive) breast cancer constitutes 70% of total breast cancer patients. In an attempt to develop a targeted therapeutic for this cancer indication, we have identified and characterized Glial cell line–Derived Neurotrophic Factor (GDNF) Family Receptor Alpha 1 (GFRA1) antibody–drug conjugates (ADC) using a cleavable valine-citrulline-MMAE (vcMMAE) linker-payload. RNAseq and IHC analysis confirmed the abundant expression of GFRA1 in luminal A breast cancer tissues, whereas minimal or no expression was observed in most normal tissues. Anti–GFRA-vcMMAE ADC internalized to the lysosomes and exhibited target-dependent killing of GFRA1-expressing cells both in vitro and in vivo. The ADCs using humanized anti-GFRA1 antibodies displayed robust therapeutic activity in clinically relevant cell line–derived (MCF7 and KPL-1) tumor xenograft models. The lead anti-GFRA1 ADC cross-reacts with rodent and cynomolgus monkey GFRA1 antigen and showed optimal pharmacokinetic properties in both species. These properties subsequently enabled a target-dependent toxicity study in rats. Anti-GFRA1 ADC is well tolerated in rats, as seen with other vcMMAE linker–payload based ADCs. Overall, these data suggest that anti–GFRA1-vcMMAE ADC may provide a targeted therapeutic opportunity for luminal A breast cancer patients. Mol Cancer Ther; 17(3); 638–49. ©2017 AACR.