bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decreased histone H3K9 dimethylation in synergy with DNA demethylation of Spi‐1 binding site contributes to ADAMTS‐5 expression in articular cartilage of osteoarthritis mice
Authors: Yan Shuaichen , Lu Tongxin , Yang Huapu , Ma Liang , Zhang Yuankai , Li Deqiang 
Published: September 2024

AbstractOsteoarthritis (OA) is defined by articular cartilage degeneration, synovial membrane inflammation, and abnormal bone remodeling. Recent study has discovered that OA development is linked to an aberrant epigenetic modification of OA‐related genes. Our previous research showed that DNA demethylation in ADAMTS‐5 promoter region had a substantial impact on ADAMTS‐5 expression in the mouse OA model. This process facilitated the binding of Spi‐1 to ADAMTS‐5 promoter. While alterations in histone methylation have been documented during embryonic development and cancer development, there is a paucity of data on the change in OA pathogenesis. Even no data have been reported on the role of histone modifications in ADAMTS‐5 activation in OA. Following our previous study on the role of DNA methylation, we aimed to examine the contribution of histone H3K9 dimethylation in ADAMTS‐5 activation in OA. Additionally, we aimed to elucidate the molecular mechanisms underlying the cooperative interaction between DNA methylation and histone H3K9 dimethylation. The potential for anti‐OA intervention therapy which is based on modulating histone H3K9 dimethylation is also explored. We demonstrated that a reduction in histone H3K9 dimethylation, along with DNA demethylation of the Spi‐1 binding site, had a role in ADAMTS‐5 activation in the articular cartilage of OA mice. Significantly, the conditional deletion of histone demethylase to be identified as lysine‐specific demethylase 1 (LSD1) in articular cartilage could alleviate the degenerative features of OA mice. Our study demonstrates the direct impact of histone H3K9 dimethylation on gene expression, which in turn contributes to OA development. This research enhances our understanding of the underlying causes of OA.
Younger age, hyperextended knee, concomitant meniscectomy and large prerevision anterior tibial translation are associated with graft failure after the revision anterior cruciate ligament reconstruction
Authors: Tokura Takeo , Matsushita Takehiko , Nishida Kyohei , Nagai Kanto , Kanzaki Noriyuki , Hoshino Yuichi , Matsumoto Tomoyuki , Kuroda Ryosuke 
Published: September 2024

AbstractPurposeGraft failure following revision anterior cruciate ligament (ACL) reconstruction is higher than after primary ACL reconstruction. However, data regarding revision surgery is scarce. We aimed to evaluate the associated factors for failure after revision ACL reconstruction.MethodsFifty‐four patients (mean age: 24.7 ± 10.0 years) who underwent revision ACL reconstruction at our hospital with ≥1 year follow‐up were retrospectively examined. Patients were divided into Group F (graft failure) and Group N (no graft failure) groups. Univariate analysis was conducted to identify factors associated with graft failure. Receiver operating characteristic (ROC) curve analysis was performed to determine the optimal thresholds for differentiating between the two groups.ResultsGraft failure was observed in 7 of 54 patients (13.0%). In the univariate analysis, significant differences were observed for age at the initial surgery (Group F: 15.6 ± 1.5, Group N: 20.9 ± 8.1), age at the revision surgery (Group F: 18.0 ± 2.8, Group N: 25.7 ± 10.3), presence of hyperextended knee (Group F: 85.7%, Group N: 14.9%), concomitant meniscectomy (Group F: 42.9%, Group N: 14.9%), prerevision space for the ACL (sACL) (Group F: 7.2 ± 3.4 mm, Group N: 13.4 ± 4.7 mm) and preoperative anterior tibial translation (ATT) (Group F: 5.0 ± 1.4 mm, Group N: 2.7 ± 3.1 m). ROC analysis of preoperative sACL and preoperative ATT on one‐leg standing plain radiograph showed that cutoff values of 6.9 and 4.2 mm were the optimal thresholds, respectively.ConclusionYounger patients with a hyperextended knee, concomitant meniscectomy, small sACL and large ATT before revision ACL reconstruction are predisposed to graft failure.Level of EvidenceLevel IV.
Is corruption an input material for environmental pollution?
Authors: Van Hung Hoang , Doanh Nguyen Khanh 
Published: September 2024

AbstractThis study examines the impact of corruption on environmental quality, covering aspects such as climate change, environmental health, air quality, sanitation and drinking water, heavy metals, and waste management across 163 countries from 2005 to 2020. We utilize a spatial autoregressive (SAR) model for panel data to analyse the spatial dependencies among these environmental quality indicators. Our findings highlight several key insights. First, there are spatial spillover effects in regional environmental quality, particularly concerning climate change, air quality, and waste management. Second, a country's environmental quality is influenced not only by its own corruption control measures but also by those of its neighbouring countries. Finally, our analysis identifies variations across different income groups: while control of corruption significantly and positively impacts environmental quality in high‐income and medium‐income countries, its effect is positive but statistically insignificant in low‐income countries. These results emphasize the need for tailored environmental policies for each income group and environmental aspect.
Microsecond Molecular Dynamics Simulation to Gain Insight Into the Binding of MRTX1133 and Trametinib With KRASG12D Mutant Protein for Drug Repurposing
Authors: Ancy Iruthayaraj , Penislusshiyan Sakayanathan , Ameen Fuad , Chitra Loganathan 
Published: September 2024

ABSTRACTThe Kirsten Rat Sarcoma (KRAS) G12D mutant protein is a primary driver of pancreatic ductal adenocarcinoma, necessitating the identification of targeted drug molecules. Repurposing of drugs quickly finds new uses, speeding treatment development. This study employs microsecond molecular dynamics simulations to unveil the binding mechanisms of the FDA‐approved MEK inhibitor trametinib with KRASG12D, providing insights for potential drug repurposing. The binding of trametinib was compared with clinical trial drug MRTX1133, which demonstrates exceptional activity against KRASG12D, for better understanding of interaction mechanism of trametinib with KRASG12D. The resulting stable MRTX1133‐KRASG12D complex reduces root mean square deviation (RMSD) values, in Switch I and II domains, highlighting its potential for inhibiting KRASG12D. MRTX1133's robust interaction with Tyr64 and disruption of Tyr96‐Tyr71‐Arg68 network showcase its ability to mitigate the effects of the G12D mutation. In contrast, trametinib employs a distinctive binding mechanism involving P‐loop, Switch I and II residues. Extended simulations to 1 μs reveal sustained network interactions with Tyr32, Thr58, and GDP, suggesting a role of trametinib in maintaining KRASG12D in an inactive state and impede the further cell signaling. The decomposition binding free energy values illustrate amino acids' contributions to binding energy, elucidating ligand–protein interactions and molecular stability. The machine learning approach reveals that van der Waals interactions among the residues play vital role in complex stability and the potential amino acids involved in drug–receptor interactions of each complex. These details provide a molecular‐level understanding of drug binding mechanisms, offering essential knowledge for further drug repurposing and potential drug discovery.
Education and Training in Peritoneal Surface Oncology
Authors: González‐Moreno Santiago , Deraco Marcello , Kusamura Shigeki , Bijelic Lana , Lambert Laura A. , Dromain Clarisse , Bibeau Frédéric , Liauw Winston , Reis Artur , Galan Ana , Sugarbaker Paul H. 
Published: September 2024

ABSTRACTPeritoneal surface oncology (PSO) is a novel field dealing with the knowledge of peritoneal neoplasms, primary or secondary, and their clinical management. As a specific treatment with curative intent for peritoneal neoplasms developed over the years, there is a growing need to comprehensively educate and train surgical oncologists worldwide in this discipline, a recognized unmet need. The European School of Peritoneal Surface Oncology (ESPSO) emerged in 2014 to provide an answer to this need.
Quality of Life Post Cervical Cancer Treatment: A Comparison Between Radical Surgery Approach and Radiotherapy and Chemotherapy
Authors: Tokarski Alyne O. , Bernardelli Rafaella S. , Bruscato Julia R. , Lucena Claudio R. , Linhares Jose C. , Tsunoda Audrey T. 
Published: September 2024

ABSTRACTIntroductionThe assessment of quality of life (QoL) in women with cervical cancer is crucial due to the profound changes they undergo during and after treatment. Often, the significance of sexual factors is underestimated, likely due to societal taboos surrounding such discussions.ObjectiveThis study aimed to determine the long‐term QoL outcomes, particularly focusing on sexuality, among three therapeutic approaches for cervical cancer: chemotherapy, radiotherapy, and brachytherapy; isolated hysterectomy; and hysterectomy combined with radiotherapy.MethodsConducted from November 2022 to July 2023, this cross‐sectional study involved 131 cervical cancer patients. Their QoL was assessed using the MDASI, FACIT‐Cx, and risk factor questionnaires. Results were compared across the three treatment groups, revealing notable differences.ResultsPatients undergoing chemo/radio/brachytherapy showed significantly lower QoL scores compared to those undergoing isolated hysterectomy. This was evident in reduced scores across FACIT‐Cx subscales for physical well‐being, specific concerns, and FACIT‐total (p < 0.05). The MDASI results similarly indicated greater symptoms and interference in daily activities for the chemo/radio/brachytherapy group.ConclusionIn conclusion, isolated hysterectomy, demonstrated superior QoL outcomes compared to chemo/radio/brachytherapy. Furthermore, the study underscored the importance of addressing sexual concerns in QoL assessments of cervical cancer survivors, emphasizing the need for comprehensive care to enhance overall well‐being posttreatment.
Examining the impact of brief couples‐based posttraumatic stress disorder treatments on anger and psychological aggression in veterans and their partners
Authors: Wells Stephanie Y. , Knopp Kayla , Wachsman Tamara R. , Dillon Kirsten H. , Walker Hannah E. , Sippel Lauren , Morland Leslie , Glassman Lisa H. 
Published: September 2024

AbstractAnger can adversely impact functioning in veterans. Psychological aggression, which is related to but distinct from anger, is particularly detrimental to veterans’ mental health. Research examining anger and psychological aggression following individual therapy for posttraumatic stress disorder (PTSD) has demonstrated small effect sizes. Treatments that directly target conflict management and interpersonal functioning, both regarding content and delivery to veterans and their loved ones (e.g., couples‐based PTSD treatments), may be more effective in alleviating anger symptoms. This study examined whether larger reductions in anger and psychological aggression would be observed in a couples‐based intervention compared to an active comparator at posttreatment and follow‐up. Data were derived from a randomized trial comparing brief cognitive–behavioral conjoint therapy for PTSD (bCBCT) and PTSD family education (PFE). Participants were 137 veterans and their intimate partners (bCBCT: n = 92, PFE: n = 45). We observed within‐condition significant reductions in angry temperament, d = ‐0.47, p < .001, and angry reaction, d = −0.26, p = .004, among veterans in bCBCT but not PFE, |d|s = 0.13–0.17, ps = .166–.268. Veterans and partners in both conditions reported reductions in psychological aggression, |d|s = 1.09–1.46, ps < .001. There were no significant differences between the treatment conditions on any outcome, ps = .103–.443, and there were no significant changes in anger between posttreatment and follow‐up, |d|s = 0.07–0.24, ps = .052–.582. Couples‐based interventions for PTSD, including bCBCT and PFE, can be effective in improving aspects of anger among veterans and their intimate partners.
Microbial Community and Functions Depending on Tillage and Straw Returning Management: Consequences for Soil Health and Ecosystem Services
Authors: Liu Qing , Dai Hongcui , Wang Liang , Qian Xin , Gao Yingbo , Zhang Hui , Liu Kaichang , Li Zongxin , Zamanian Kazem 
Published: September 2024

ABSTRACTTillage and straw returning are promising practices to improve soil quality, especially because of the unclear controlling effects on microbial communities and functions. A 5‐year field experiment in a winter wheat‐summer maize cropping system was implemented in the North China Plain to address this research gap. Management practices were deep tillage, rotary tillage, and no‐tillage, each with either wheat only or both wheat and maize straw returning. Shotgun metagenomic sequencing was performed to investigate the microbial community, diversity, co‐occurrence network, and function in the topsoil (0–20 cm). The associations between soil physiochemical properties and microbial community characteristics were also evaluated. Rotary tillage with only wheat straw returning (RTS) significantly increased Shannon's diversity index by 0.24%–1.71% compared to other treatments. No‐tillage with only wheat straw returning (NTS) showed the most stable microbial network with the highest betweenness centrality (199.09), average path distance (2.31), and modularity (0.50). NTS had the highest relative abundance of microbial carbon (C), nitrogen (N) metabolisms, and C fixation pathways. Among deep tillage practices, both wheat and maize straw returning (DTD) were more beneficial to the stability of microbial networks than only wheat straw returning (DTS), yet without improvement in microbial diversity and function. In conclusion, microbial community and function are practical predictors of variations in soil nutrient availability, and pH value in response to different tillage and residue management practices. In particular, our study provides a basis for the development of a sustainable crop residue management system.
High‐Shear Enhancement of Biginelli Reactions in Macromolecular Viscous Media
Authors: Bui Aaron Hung , Rowlands Naomi Beth , Fernando Pulle Anne Dilpashani , Gibbs Medina Sam Andrés , Rohrsheim Tullia Jade , Tuten Bryan Tyler 
Published: September 2024

AbstractChemical reactions and transformations in non‐traditional vessels have gained significant interest in recent years. Flow chemistry, with its advantages in mixing, mass transfer, scalability, and automation, is a driving force behind this paradigm shift. In particular, the Vortex Fluidic Device (VFD) has emerged as a versatile tool across various applications, from organic synthesis to materials science. In this study, the role of the VFD in performing the Biginelli reaction, a multicomponent reaction widely used in pharmaceutical and polymer science, for a post‐polymerization modification is explored. By conducting the Biginelli reaction in the VFD, rapid product formation with low catalyst loading and without the need for high temperatures is achieved. However, the critical need to understand and know solution viscosity, especially within the context of modifying macromolecules is highlighted.
ω‐3 PUFAs Ameliorated the Maternal High‐Fructose Diet‐Induced Early‐Onset Retinopathy in Offspring via Inhibiting NLRP3‐Associated Neuroinflammation
Authors: Huang Hsiu‐Mei , Chang Ying‐Chao , Lee Jong‐Jer , Huang Yao‐Sheng , Wu Kay L. H. 
Published: September 2024

ScopeMaternal high fructose diet (HFD) during pregnancy and lactation can initiate retinal dysfunction. However, the underlying mechanism remains largely unknown.Methods and resultsBy using the rodent model of maternal HFD in this study, the results from electroretinography (ERG) indicate that b‐wave amplitude, an index of inner retinal function, is significantly reduced as early as 3 months old and the deteriorated effect can be detected at 15 months old. Further, the protein expressions of CD11b (a marker of active microglia), p40phox subunit of NADPH oxidase, GFAP (a marker of active astrocytes), and NLPR3 examined by western blot and immunofluorescence are significantly increased in the retina of the male HFD offspring at 3 months old. Treatment with omega‐3 polyunsaturated fatty acids (ω‐3 PUFAs) for 2 weeks (from 2.5 to 3 months old) effectively reverses the aforementioned changes.ConclusionTogether, these results indicate that the early onset and extensive retinal dysfunction may be a result of glial activation which is induced by maternal HFD to initiate an inflammatory microenvironment leading to a long‐term progression of retinopathy. Short‐term administration of ω‐3 PUFA at a young age may be a feasible strategy to intervene in the maternal HFD‐programmed retinal impairment in male offspring.
Monomethyl Branched‐Chain Fatty Acids Suppress M1 Macrophage Polarization via FABP4/PPAR‐γ Signaling Pathway
Authors: He Yuan , Zhang Yu , Zhu Shuang , Liu Yuan‐fa , Liu Sha , Xu Yong‐jiang 
Published: September 2024

ScopeMonomethyl‐branched chain fatty acids (mmBCFAs) are found in a variety of food sources and are of great interest due to their potent antiinflammatory properties. However, most of the current researches have concentrated on the relationship between mmBCFAs and intestinal inflammation, and there is a large gap in the biological mechanisms involved behind their antiinflammatory effects.Methods and resultsThe present study examines the role of mmBCFAs in modulating macrophage polarization. The results demonstrate that iso‐C16:0 significantly inhibits macrophages M1 proinflammatory polarization through regulating FABP4/PPAR‐γ pathway. Proteomics and molecular biology experiments verify that metabolic reprogramming is involved in the inhibition of M1 macrophage, referring to the upregulation of fatty acid oxidation, TCA cycle, and oxidative phosphorylation, as well as downregulation of glycolytic flux.ConclusionIn summary, this study offers a novel perspective on the antiinflammatory effects mediated by mmBCFAs.
Coronary atherosclerotic plaque characterization with silicon‐based photon‐counting computed tomography (CT): A simulation‐based feasibility study
Authors: Li Mengzhou , Wu Mingye , Pack Jed , Wu Pengwei , Yan Pingkun , De Man Bruno , Wang Adam , Nieman Koen , Wang Ge 
Published: September 2024

AbstractBackgroundRecent photon‐counting computed tomography (PCCT) development brings great opportunities for plaque characterization with much‐improved spatial resolution and spectral imaging capability. While existing coronary plaque PCCT imaging results are based on CZT‐ or CdTe‐materials detectors, deep‐silicon photon‐counting detectors offer unique performance characteristics and promise distinct imaging capabilities.PurposeThis study aims to numerically investigate the feasibility of characterizing plaques with a deep‐silicon PCCT scanner and to demonstrate its potential performance advantages over traditional CT scanners using energy‐integrating detectors (EID).MethodsWe conducted a systematic simulation study of a deep‐silicon PCCT scanner using a newly developed digital plaque phantom with clinically relevant geometrical and chemical properties. Through qualitative and quantitative evaluations, this study investigates the effects of spatial resolution, noise, and motion artifacts on plaque imaging.ResultsNoise‐free simulations indicated that PCCT imaging could delineate the boundary of necrotic cores with a much finer resolution than EID‐CT imaging, achieving a structural similarity index metric (SSIM) score of 0.970 and reducing the root mean squared error (RMSE) by two‐thirds. Measuring necrotic core area errors were reduced from 91.5% to 24%, and fibrous cap thickness errors were reduced from 349.8% to 33.3%. In the presence of noise, the optimal reconstruction was achieved using 0.25 mm voxels and a soft reconstruction kernel, yielding the highest contrast‐to‐noise ratio (CNR) of 3.48 for necrotic core detection and the best image quality metrics among all choices. However, the ultrahigh resolution of PCCT increased sensitivity to motion artifacts, which could be mitigated by keeping residual motion amplitude below 0.4 mm.ConclusionsThe findings suggest that deep‐silicon PCCT scanner can offer sufficient spatial resolution and tissue contrast for effective plaque characterization, potentially improving diagnostic accuracy in cardiovascular imaging, provided image noise and motion blur can be mitigated using advanced algorithms. This simulation study involves several simplifications, which may result in some idealized outcomes that do not directly translate to clinical practice. Further validation studies with physical scans are necessary and will be considered for future work.
Differentiating leukemia subtypes based on metabolic signatures using hyperpolarized 13C NMR
Authors: Christensen Nichlas Vous , Laustsen Christoffer , Bertelsen Lotte Bonde 
Published: September 2024

AbstractLeukemia is a group of blood cancers that are classified in four major classes. Within these four classes, many different subtypes exists with similar origin, genetic mutations, and level of maturity, which can make them difficult to distinguish. Despite their similarities, they might respond differently to treatment, and therefore distinguishing between them is of crucial importance. A deranged metabolic phenotype (Warburg effect) is often seen in cancer cells, leukemia cells included, and is increasingly a target for improved diagnosis and treatment. In this study, hyperpolarized 13C NMR spectroscopy was used to characterize the metabolic signatures of the six leukemia cell lines ML‐1, CCRF‐CEM, THP‐1, MOLT‐4, HL‐60, and K562. This was done using [1‐13C]pyruvate and [1‐13C]alanine as bioprobes for downstream metabolite quantification and kinetic analysis on cultured cells with and without 2‐deoxy‐D‐glucose treatment. The metabolic signatures of similar leukemia subtypes could be readily distinguished. This includes ML‐1 and THP‐1, which are of the similar M4 and M5 AML subtypes, CCRF‐CEM and MOLT‐4, which are of the similar T‐ALL lineage at different maturation states, and HL‐60 and K562, which are of the closely related M1 and M2 AML subtypes. The data presented here demonstrate the potential of hyperpolarized 13C NMR spectroscopy as a method to differentiate between leukemia subtypes of similar origin. Combining this method with bioreactor setups could potentially allow for better leukemia disease management as metabolic signatures could be acquired from a single biopsy through repeated experimentation and intervention.