bluuebunny/crossref_metadata_2025_split huggingface dataset:
МРТ-морфометрия тел позвонков и межпозвоночных дисков поясничного отдела позвоночника у больных с нарушением минеральной плотности костной ткани
Authors: Sharmazanova Ye.P. , Miahkov S.A. , Rybak I.R. 
Published: December 2016

Цель исследования — изучение изменений тел позвонков, межпозвоночных дисков и их площадей в поясничном отделе позвоночника при уменьшении минеральной плотности костной ткани (МКП) с помощью магнитно-резонансной томографии (МРТ). 81 пациенту были проведены двухэнергетическая рентгеновская денситометрия (ДРД) и МРТ поясничного отдела позвоночника. Остеопения диагностирована у 33 больных (ІІ группа), остеопороз — у 28 (ІІІ группа), контрольную группу составили 20 пациентов (І группа) без признаков остеопороза (по данным ДРД). Cредние показатели МКП (г/см2) в телах L1-L4 составили: у здоровых — 1,232 ± 0,060; при остеопении — –1,032 ± 0,070; при остеопорозе — –0,757 ± 0,080. Средние показатели Т-критерия в группе сравнения составили –1,27 ± 0,71; во ІІ группе с остеопенией — –1,40 ± 011; в ІІІ группе с остеопорозом — –3,09 ± 1,73. Разница в МКП между I и II группами составила 16,2 % (р < 0,05), между I и III группами — 25 % (p < 0,01). При МРТ-морфометрии у больных с остеопенией и остеопорозом изменения тел позвонков поясничного отдела позвоночника сопровождались снижением средней высоты тел, незначительным снижением передней высоты тел позвонков; уменьшением индекса Барнетта — Нордина; выраженным увеличением средней высоты и площадей межпозвоночных дисков, более выраженными при остеопорозе. Установлено, что у больных с нарушением минеральной плотности костной ткани имеются и дегенеративные изменения межпозвоночных дисков, причем степень их дегенерации достоверно не отличается от группы контроля.
Тези міжнародного симпозіуму «Захворювання кістково-м’язової системи та вік», м. Львів, 5–7 травня, 2015
Authors: Andrusha A. , Bakalyuk T.G. , Barbosa et all Ana Paula 
Published: December 2016

Наведені тези міжнародного симпозіуму «Захворювання кістково-м’язової системи та вік», що відбувся 5–7 травня 2015 року у м. Львів, Україна
Вікові та статеві особливості геометрії проксимального відділу стегнової кістки у хворих із її внутрішньосуглобовими переломами
Authors: Grygorieva N.V. , Zubach O.B. 
Published: December 2016

Згідно з даними літературних джерел, деякі параметри стегнової кістки (СК), а саме довжина її осі, шийково-діафізарний кут та ін., є незалежними предикторами переломів проксимального відділу стегнової кістки (ПВ СК), проте подібні дослідження серед хворих української популяції відсутні. У статті наведені дані літературних джерел та результати власного дослідження особливостей геометричних параметрів СК у хворих із внутрішньосуглобовими переломами ПВ СК. Проаналізовано 100 оглядових (передньо­задніх) рентгено­грам кульшових суглобів (таза) хворих віком 50–89 років (середній вік 70,98 ± 0,99 року). Обстежено 31 жінку та 39 чоловіків, які були госпіталізовані з внутрішньо­суглобовими переломами СК, та 30 осіб (16 жінок і 14 чоловіків) без переломів. Оцінку параметрів геометрії стегнової кістки проводили на контралатеральній до перелому кінцівці. Для аналізу пацієнти були розподілені на підгрупи за віком і статтю. Виявлено вірогідний вплив віку на параметри геометрії ПВ СК у чоловіків та жінок із внутрішньосуглобовими переломами, проте не в осіб без переломів. Також встановлено вірогідно нижчі показники довжини осі стегнової кістки, основи та діаметра головки, відстані «головка — вертлюг» у чоловіків із внутрішньосуглобовими переломами ПВ СК порівняно з показниками осіб без переломів при відсутності вірогідних відмінностей показників у жінок. Виявлені відмінності необхідно враховувати як при плануванні оперативних втручань після перелому ПВ СК, так і для прогнозування ризику переломів ПВ СК в осіб старших вікових груп.
Pain and discomfort during the first week of rapid maxillary expansion (RME) using two different RME appliances: A randomized controlled trial
Authors: Feldmann Ingalill , Bazargani Farhan 
Published: December 2016

ABSTRACT

Objectives:
To evaluate and compare perceived pain intensity, discomfort, and jaw function impairment during the first week with tooth-borne or tooth-bone–borne rapid maxillary expansion (RME) appliances.

Materials and Methods:
Fifty-four patients (28 girls and 26 boys) with a mean age of 9.8 years (SD 1.28 years) were randomized into two groups. Group A received a conventional hyrax appliance and group B a hybrid hyrax appliance anchored on mini-implants in the anterior palate. Questionnaires were used to assess pain intensity, discomfort, analgesic consumption, and jaw function impairment on the first and fourth days after RME appliance insertion.

Results:
Fifty patients answered both questionnaires. Overall median pain on the first day in treatment was 13.0 (range 0–82) and 3.5 (0–78) for groups A and B, respectively, with no significant differences in pain, discomfort, analgesic consumption, or functional jaw impairment between groups. Overall median pain on the fourth day was 9.0 (0–90) and 2.0 (0–71) for groups A and B, respectively, with no significant differences between groups. There were also no significant differences in pain levels within group A, while group B scored significantly lower concerning pain from molars and incisors and tensions from the jaw on day 4 than on the first day in treatment. There was a significant positive correlation between age and pain and discomfort on the fourth day in treatment. No correlations were found between sex and pain and discomfort, analgesic consumption, and jaw function impairment.

Conclusions:
Both tooth-borne and tooth-bone–borne RME were generally well tolerated by the patients during the first week of treatment.
Effects of prophylactic incisional gastropexy on markers of gastric motility in dogs as determined by use of a novel wireless motility device
Authors: Gazzola Krista M. , Nelson Laura L. , Fritz Michele C. , Clancy Michelle R. , Hauptman Joe G. 
Published: December 2016

Abstract
OBJECTIVE
To evaluate effects of laparoscopic-assisted incisional gastropexy (LAIG) on gastric motility in dogs by use of a wireless motility device (WMD).
ANIMALS
10 healthy client-owned large or giant-breed dogs.
PROCEDURES
10 dogs owned by clients interested in prophylactic LAIG were enrolled. To determine effects of LAIG on gastrointestinal motility in dogs during the nonfed state, each dog was evaluated by use of a noninvasive WMD before and > 4 weeks after LAIG. All dogs underwent LAIG, with or without concurrent elective gonadectomy. Data obtained before and after LAIG were analyzed by use of proprietary software to determine the gastric emptying time, small bowel transit time, large bowel transit time, whole bowel transit time, and motility index.
RESULTS
No changes in variables were detected between measurements obtained before and after prophylactic LAIG.
CONCLUSIONS AND CLINICAL RELEVANCE
In this study, prophylactic LAIG did not have an effect on gastrointestinal motility. The WMD was tolerated well by all dogs and appeared to be a safe and effective method for evaluating gastrointestinal motility in this population of dogs.
Effect of fentanyl and lidocaine on the end-tidal sevoflurane concentration preventing motor movement in dogs
Authors: Suarez Martin A. , Seddighi Reza , Egger Christine M. , Rohrbach Barton W. , Cox Sherry K. , KuKanich Butch K. , Doherty Thomas J. 
Published: December 2016

Abstract
OBJECTIVE To determine effects of fentanyl, lidocaine, and a fentanyl-lidocaine combination on the minimum alveolar concentration of sevoflurane preventing motor movement (MACNM) in dogs.
ANIMALS 6 adult Beagles.
PROCEDURES Dogs were anesthetized with sevoflurane in oxygen 3 times (1-week intervals). Baseline MACNM (MACNM-B) was determined starting 45 minutes after induction of anesthesia. Dogs then received 1 of 3 treatments IV: fentanyl (loading dose, 15 μg/kg; constant rate infusion [CRI], 6 μg/kg/h), lidocaine (loading dose, 2 mg/kg; CRI, 6 mg/kg/h), and the fentanyl-lidocaine combination at the same doses. Determination of treatment MACNM (MACNM-T) was initiated 90 minutes after start of the CRI. Venous blood samples were collected at the time of each treatment MACNM measurement for determination of plasma concentrations of fentanyl and lidocaine.
RESULTS Mean ± SEM overall MACNM-B for the 3 treatments was 2.70 ± 0.27 vol%. The MACNM decreased from MACNM-B to MACNM-T by 39%, 21%, and 55% for fentanyl, lidocaine, and the fentanyl-lidocaine combination, respectively. This decrease differed significantly among treatments. Plasma fentanyl concentration was 3.25 and 2.94 ng/mL for fentanyl and the fentanyl-lidocaine combination, respectively. Plasma lidocaine concentration was 2,570 and 2,417 ng/mL for lidocaine and the fentanyl-lidocaine combination, respectively. Plasma fentanyl and lidocaine concentrations did not differ significantly between fentanyl and the fentanyl-lidocaine combination or between lidocaine and the fentanyl-lidocaine combination.
CONCLUSIONS AND CLINICAL RELEVANCE CRIs of fentanyl, lidocaine, and the fentanyl-lidocaine combination at the doses used were associated with clinically important and significant decreases in the MACNM of sevoflurane in dogs.
Pharmacokinetics of intravenous continuous rate infusions of sodium benzylpenicillin and ceftiofur sodium in adult horses
Authors: Edwards Scott H. , Khalfan Shahid A. , Jacobson Glenn A. , Pirie Adam D. , Raidal Sharanne L. 
Published: December 2016

Abstract
OBJECTIVE To determine plasma drug concentrations after IV administration of a bolus followed by continuous rate infusion (CRI) of sodium benzylpenicillin and ceftiofur sodium to healthy adult horses.
ANIMALS 6 Thoroughbred mares (3 to 9 years old; mean ± SD body weight, 544 ± 55 kg) with no history of recent antimicrobial treatment.
PROCEDURES Horses were used in 2 experiments conducted 14 days apart. For each experiment, horses were housed individually in stables, and catheters were placed bilaterally in both jugular veins for drug administration by CRI (left catheter) and for intermittent collection of blood samples (right catheter). Synovial fluid samples were obtained from carpal joints following ceftiofur administration to evaluate drug diffusion into articular spaces.
RESULTS Plasma concentrations above accepted minimum inhibitory concentrations for common pathogens of horses were achieved within 1 minute after bolus administration and remained above the minimum inhibitory concentration for 48 (ceftiofur) or 12 (benzylpenicillin) hours (ie, the duration of the CRI). Mean synovial fluid ceftiofur free acid equivalent concentrations were approximately 46% (range, 25.4% to 59.8%) of plasma concentrations at the end of infusion.
CONCLUSIONS AND CLINICAL RELEVANCE Compared with intermittent bolus administration, the loading dose and CRI used less drug but maintained high plasma concentrations for the duration of infusion. By use of pharmacological parameters derived in this study, a loading dose of 2.5 mg/kg and CRI of 200 μg/kg/h should achieve plasma ceftiofur concentrations of 4 μg/mL; a loading dose and CRI of 1.3 mg/kg and 2.5 μg/kg/h, respectively, should achieve plasma benzylpenicillin concentrations of 2 μg/mL.
Assessment of eosinophil peroxidase as a potential diagnostic and prognostic marker in dogs with inflammatory bowel disease
Authors: Bastan Idil , Robinson Nicholas A. , Ge Xiao Na , Rendahl Aaron K. , Rao Savita P. , Washabau Robert J. , Sriramarao P. 
Published: December 2016

Abstract
OBJECTIVE To evaluate a method for identifying intact and degranulated eosinophils in the small intestine of dogs with inflammatory bowel disease (IBD) by use of a monoclonal antibody (mAb) against eosinophil peroxidase (EPX).
ANIMALS 11 untreated dogs with IBD, 5 dogs with IBD treated with prednisolone, and 8 control dogs with no clinical evidence of gastrointestinal tract disease and no immunosuppressive treatment.
PROCEDURES 4-μm-thick sections of paraffin-embedded tissues from necropsy specimens were immunostained with EPX mAb. Stained intact and degranulated eosinophils in consecutive microscopic fields (400X magnification) of the upper (villus tips) and lower (between the muscularis mucosae and crypts) regions of the lamina propria of the jejunum were manually counted.
RESULTS Compared with control and treated IBD dogs, untreated IBD dogs had a significantly higher number of degranulated eosinophils in the lower region of the lamina propria. However, no significant differences were detected in the number of intact eosinophils in this region among groups. In the upper region of the lamina propria, untreated IBD dogs had a significantly higher number of degranulated and intact eosinophils, compared with control and treated IBD dogs. Number of degranulated and intact eosinophils did not differ significantly between control and treated IBD dogs.
CONCLUSIONS AND CLINICAL RELEVANCE Immunohistologic analysis with EPX mAb yielded prominent granule staining that allowed reliable morphological identification of degranulated and intact eosinophils, which may provide a strategy for quantitative and selective evaluation of eosinophils in gastrointestinal biopsy specimens and a potential method to diagnose IBD and evaluate treatment outcome.
High-resolution melting analysis for detection of a single-nucleotide polymorphism and the genotype of the myostatin gene in warmblood horses
Authors: Serpa Priscila B. S. , Garbade Petra , Natalini Cláudio C. , Pires Ananda R. , Tisotti Tainor M. 
Published: December 2016

Abstract
OBJECTIVE To develop a high-resolution melting (HRM) assay to detect the g.66493737C>T polymorphism in the myostatin gene (MSTN) and determine the frequency of 3 previously defined g.66493737 genotypes (T/T, T/C, and C/C) in warmblood horses.
SAMPLES Blood samples from 23 horses.
PROCEDURES From each blood sample, DNA was extracted and analyzed by standard PCR methods and an HRM assay to determine the MSTN genotype. Three protocols (standard protocol, protocol in which a high-salt solution was added to the reaction mixture before the first melting cycle, and protocol in which an unlabeled probe was added to the reaction mixture before analysis) for the HRM assay were designed and compared. Genotype results determined by the HRM protocol that generated the most consistent melting curves were compared with those determined by sequencing.
RESULTS The HRM protocol in which an unlabeled probe was added to the reaction mixture generated the most consistent melting curves. The genotypes of the g.66493737C>T polymorphism were determined for 22 horses (16 by HRM analysis and 20 by sequencing); 14, 7, and 1 had the T/T, T/C, and C/C genotypes, respectively. The genotype determined by HRM analysis agreed with that determined by sequencing for 14 of 16 horses. The frequency of alleles T and C was 79.5% and 20.5%, respectively.
CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that HRM analysis may be a faster and more economical alternative than PCR methods for genotyping. Genotyping results might be useful as predictors of athletic performance for horses.
Evaluation of procoagulant tissue factor expression in canine hemangiosarcoma cell lines
Authors: Witter Lauren E. , Gruber Erika J. , Lean Fabian Z. X. , Stokol Tracy 
Published: December 2016

Abstract
OBJECTIVE To evaluate expression of procoagulant tissue factor (TF) by canine hemangiosarcoma cells in vitro.
SAMPLES 4 canine hemangiosarcoma cell lines (SB-HSA [mouse-passaged cutaneous tumor], Emma [primary metastatic brain tumor], and Frog and Dal-1 [primary splenic tumors]) and 1 nonneoplastic canine endothelial cell line (CnAoEC).
PROCEDURES TF mRNA and TF antigen expression were evaluated by quantitative real-time PCR assay and flow cytometry, respectively. Thrombin generation was measured in canine plasma and in coagulation factor–replete or specific coagulation factor–deficient human plasma by calibrated automated thrombography. Corn trypsin inhibitor and annexin V were used to examine contributions of contact activation and membrane-bound phosphatidylserine, respectively, to thrombin generation.
RESULTS All cell lines expressed TF mRNA and antigen, with significantly greater expression of both products in SB-HSA and Emma cells than in CnAoEC. A greater percentage of SB-HSA cells expressed TF antigen, compared with other hemangiosarcoma cell lines. All hemangiosarcoma cell lines generated significantly more thrombin than did CnAoEC in canine or factor-replete human plasma. Thrombin generation induced by SB-HSA cells was significantly lower in factor VII–deficient plasma than in factor-replete plasma and was abolished in factor X–deficient plasma; residual thrombin generation in factor VII–deficient plasma was abolished by incubation of cells with annexin V. Thrombin generation by SB-HSA cells was unaffected by the addition of corn trypsin inhibitor.
CONCLUSIONS AND CLINICAL RELEVANCE Hemangiosarcoma cell lines expressed procoagulant TF in vitro. Further research is needed to determine whether TF can be used as a biomarker for hemostatic dysfunction in dogs with hemangiosarcoma.
Pharmacokinetics of terbinafine in little brown myotis (Myotis lucifugus) infected with Pseudogymnoascus destructans
Authors: Court Michael H. , Robbins Alison H. , Whitford Anne M. , Beck Erika V. , Tseng Flo S. , Reeder DeeAnn M. 
Published: December 2016

Abstract
OBJECTIVE
To determine the pharmacokinetics of terbinafine in little brown myotis (Myotis lucifugus) infected with Pseudogymnoascus destructans.
ANIMALS
123 bats from a P destructans–infected hibernation site in Virginia.
PROCEDURES
3 bats were euthanized and necropsied to confirm the presence of P destructans within the population. The remaining 120 bats were systematically assigned to 6 groups (20 bats/group). Bats in each of 3 groups received 6, 20, or 60 mg of terbinafine/kg, SC, once daily for 10 days. Bats in another group received 200 mg of terbinafine/kg, SC, once daily for 5 days. Bats in 1 group received the terbinafine vehicle solution (0.1 mL/kg, SC, once daily for 10 days). Bats in the remaining group did not receive any treatment. Following the treatment period (days 1 through 10), bats were housed in a hibernation chamber and monitored daily until euthanasia on day 42, 75, or 109. Tissue specimens were collected from all bats as soon as possible after death or euthanasia to determine terbinafine concentration. Within each group and tissue type, terbinafine concentration data were pooled, and pharmacokinetic parameters were calculated by noncompartmental methods.
RESULTS
Adverse neurologic effects and a high mortality rate before day 10 were observed in bats that received the highest terbinafine dose (200 mg/kg) but not those that received lower doses. Presumed therapeutic terbinafine concentrations (≥ 2 μg/g) were maintained in skin and wing for at least 30 and 6 days in bats that received the 60 and 20 mg/kg doses, respectively, but were not achieved in most bats that received the 6 mg/kg dose. Tissue terminal half-life ranged from 14 to 22 days. Terbinafine concentration in hair was positively correlated with that in skin and wing.
CONCLUSIONS AND CLINICAL RELEVANCE
Results indicated terbinafine doses > 6 but < 200 mg/kg should be further evaluated for the treatment of P destructans–infected bats. Collection of serial hair specimens may represent a noninvasive method for monitoring terbinafine concentration in treated bats.
Effect of scion defoliation period and methods of grafting on success and survivability in mango
Authors: Akter J , Rahim MA , Haque T , Hossain MM 
Published: December 2016

This study was conducted in order to investigate the effect of scion defoliation period and methods of grafting on the success and survivability of mango (var. Amrapali) at Bangladesh Agricultural University Germplasm Centre (BAU-GPC) of Fruit Tree Improvement Program (FTIP) of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh. The experiment consisted of four scion defoliation periods, viz., defoliation before 9, 6, 3 days of grafting and defoliation on the day of grafting and two grafting methods namely cleft grafting and whip grafting. The study was laid out in randomized complete block design with three replications. The results revealed that scions defoliated 9 days before grafting showed the highest success (91.59%) and survivability (88.75%) and took the lowest time for bud breaking and first leaf opening (11.10 days and 14.22 days, respectively)whereas the lowest success rate (85.82% ) and survivability (83.60%) were observed when scion defoliation was performed on the day of grafting. In respect of grafting methods, higher graft success and survivability (89.68% and 86.89%, respectively) were found in cleft method, however, this method took longer time for bud breaking (11.92 days) and first leaf opening (15.90 days). The combined influence of scion defoliation period and grafting method exhibited the best performance on graft success and survivability when cleft grafting method was used with scion defoliated 9 days before grafting operation. Therefore the results of this investigation suggested that scions defoliated 9 days before grafting operation and cleft grafting method would be better for propagation of mango, yet the performance of cleft grafting with scion defoliated 6 days before grafting was also good with the hope of getting 85% success and survivability.Progressive Agriculture 27 (3): 242-248, 2016
Carbohydrate reserve and aerenchyma formation enhance submergence tolerance in rice
Authors: Pramanik MHR , Shelley IJ , Adhikary D , Islam MO 
Published: December 2016

An attempt was made to evaluate the submergence tolerance in rice using four Aman rice varieties viz. FR13A (local flood resistant, check), BRRI dhan 51 (HYV flood tolerant, check), BR 5 (HYV, susceptible) and a local aromatic rice,Ukunimadhu (local susceptible). Twenty days old seedlings of the rice varieties raised in earthen pots were submerged under 90 cm water depth in a submergence tank for 12 days and data were recorded on plant height, tiller number, carbohydrate reserve and internal anatomical structure of roots of the submerged and control (ambient) plants. Under submergence treatment Ukunimadhu showed rapid stem elongation with taller plants than the HYVs which showed shorter plant height. Tillering pattern was almost similar both in submergence treated and control plants in all the varieties. Variation in carbohydrate reserve was negligible among the varieties at ambient condition but the submergence treatment brought significant variation in carbohydrate content in them. Submergence treated FR13A and BRRI dhan 51 had the highest sugar and starch conservation ability while BR 5 and Ukunimadhu had the lowest. The higher reserved carbohydrate in the tolerant varieties might assist them to regenerate their normal growth rapidly after desubmergence. The cellular morphology of root tissue showed that intensity of aerenchyma development in submergence treated FR13A and BRRI dhan 51 was more pronounced compared to the local aromatic rice Ukunimadhu and perhaps this cellular large aerenchymatous development might be associated with the submergence tolerance of FR13A and BRRI dhan 51 than the local variety, Ukunimadhu.Progressive Agriculture 27 (3): 256-264, 2016