bluuebunny/crossref_metadata_2025_split huggingface dataset:
Listeria monocytogenesReplicate in Bone Marrow–Derived CD11c+ Cells but Not in Dendritic Cells Isolated from the Murine Gastrointestinal Tract
Authors: Jones Grant S. , Smith Victoria C. , D’Orazio Sarah E. F. 
Published: October 2017

AbstractRecent fate-mapping studies and gene-expression profiles suggest that commonly used protocols to generate bone marrow–derived cultured dendritic cells yield a heterogeneous mixture, including some CD11chi cells that may not have a bona fide counterpart in vivo. In this study, we provide further evidence of the discordance between ex vivo–isolated and in vitro–cultured CD11c+ cells by analyzing an additional phenotype, the ability to support cytosolic growth of the facultative intracellular bacterial pathogen Listeria monocytogenes. Two days after foodborne infection of mice with GFP-expressing L. monocytogenes, a small percentage of CD103neg and CD103+ conventional dendritic cells (cDC) in the intestinal lamina propria and mesenteric lymph nodes were GFP+. However, in vitro infection of the same subsets of cells harvested from naive mice resulted in inefficient invasion by the bacteria (&lt;0.1% of the inoculum). The few intracellular bacteria detected survived for only a few hours. In contrast, cultured CD103negCD11c+ cells induced by GM-CSF readily supported exponential growth of L. monocytogenes. Flt3 ligand–induced cultures yielded CD103+CD11c+ cells that more closely resembled cDC, with only a modest level of L. monocytogenes replication. For both culture protocols, the longer the cells were maintained in vitro, the more readily they supported intracellular growth. The results of this study suggest that cDC are not a niche for intracellular growth of L. monocytogenes during intestinal infection of mice.
Assessment of healthcare measures, healthcare resource use, and cost of care among severe hemophilia A patients in Mumbai region of India
Authors: Jadhav U , Mukherjee K 
Published: October 2017

Background:
In India, the low public health priority given to rare disorders such as hemophilia hinders their management and optimal care, leading to relatively poor health outcomes. This study aims to profile the multidimensional health status of patients with severe hemophilia A, and its association with the use of healthcare resources and the cost of care in Mumbai region of India.

Subjects and Methods:
A cross-sectional, single-center study was conducted during January–May 2011, among 160 patients diagnosed with severe hemophilia A in Mumbai region of India. Their health status was documented using the Hemophilia Utilization Group Study's validated instrument of Functional Health Status Measure (FHS) and a single item of Self-care Measure.

Results:
Of 160 patients, 55% (n = 88) scored on the lower side on the FHS, with an average score of 6.65 ± 2.85. The use of healthcare resources and cost of treatment were considerable for patients with a lower mean rank score on the FHS and a higher mean rank score on the self-care measure. The consumption of clotting factor concentrates (CFCs), number of visits to a health facility and incidence of inpatient episodes were significantly associated with a relatively low score on the FHS. Similarly, a higher cost of treatment, in terms of the cost of CFCs, direct cost, emergency room cost, and indirect cost, were significantly associated with a lower score on the FHS.

Conclusion:
The health status of patients with severe hemophilia A is compromised and has a significant impact on the use of healthcare resources and the cost of treatment.
Connected Numbers and the Embedded Topology of Plane Curves
Authors: Shirane Taketo 
Published: October 2017

AbstractThe splitting number of a plane irreducible curve for a Galois cover is effective in distinguishing the embedded topology of plane curves. In this paper, we define the connected number of a plane curve (possibly reducible) for a Galois cover, which is similar to the splitting number. By using the connected number, we distinguish the embedded topology of Artal arrangements of degree b ≥ 4, where an Artal arrangement of degree b is a plane curve consisting of one smooth curve of degree b and three of its total inflectional tangents.
Effect of soil saturation on denitrification in a grassland soil
Authors: Cardenas Laura Maritza , Bol Roland , Lewicka-Szczebak Dominika , Gregory Andrew Stuart , Matthews Graham Peter , Whalley William Richard , Misselbrook Thomas Henry , Scholefield David , Well Reinhard 
Published: October 2017

Abstract. Nitrous oxide (N2O) is of major importance as a greenhouse gas and precursor of ozone (O3) destruction in the stratosphere mostly produced in soils. The soil-emitted N2O is generally predominantly derived from denitrification and, to a smaller extent, nitrification, both processes controlled by environmental factors and their interactions, and are influenced by agricultural management. Soil water content expressed as water-filled pore space (WFPS) is a major controlling factor of emissions and its interaction with compaction, has not been studied at the micropore scale. A laboratory incubation was carried out at different saturation levels for a grassland soil and emissions of N2O and N2 were measured as well as the isotopocules of N2O. We found that flux variability was larger in the less saturated soils probably due to nutrient distribution heterogeneity created from soil cracks and consequently nutrient hot spots. The results agreed with denitrification as the main source of fluxes at the highest saturations, but nitrification could have occurred at the lower saturation, even though moisture was still high (71 % WFSP). The isotopocules data indicated isotopic similarities in the wettest treatments vs. the two drier ones. The results agreed with previous findings where it is clear there are two N pools with different dynamics: added N producing intense denitrification vs. soil N resulting in less isotopic fractionation.
The effects of intervention on the quality of life, sense of coherence and daily activities of frequent attenders
Authors: Hirsikangas Sari , Kanste Outi , Korpelainen Juha , Bloigu Risto , Kyngäs Helvi 
Published: October 2017

Background and objective: When FAs with chronic disease and various needs are able to keep up their psychological and physical wellbeing, perform daily activities and maintain control of their life, they are able to live an active life and take care of themselves longer, and this has an impact on their quality of life. The role of community matron is to assess an individual’s problems and needs for health services and improve the self-care of FAs by providing a needs-based education. The goal of this study is to evaluate the effectiveness of nurse-led intervention on the quality of life, sense of coherence and daily activities of frequent attenders (FAs).Methods: This study is an intervention trial, with a two-year follow-up, including intervention and control groups. The intervention group received nurse-led intervention that included the assessment of FAs care needs, an individualized care plan, support for self-management and service coordination. The FAs in the control group received traditional care, including patient education and support. Self-reported instruments were used to collect data from adult frequent attenders, including the Frenchay Activities Index (FAI), Sense of Coherence (SOC) and 15D, to assess the health-related quality of life (HRQoL). Follow-ups measurements were taken after one year and after two years between 2008 and 2011.Results: The study shows a significant correlation between sense of coherence and quality of life (p < .0005) and quality of life and amount of daily activities performed (p < .0005) in both groups. The intervention group had statistically significantly better sense of coherence at every stage of the study. The activities of daily living statistically significantly weakened in the intervention group (p = .001) from baseline to two years despite intervention, whereas in the control group it remained unchanged. In the control group, a statistically significant correlation was found between sense of coherence and FAs feeling satisfaction with their current life (p = .019). The HRQoL did not differ between the groups during the study.Conclusions: These results indicate a correlation between sense of coherence and quality of life and also between quality of life and amount of daily activities among FAs. Intervention did not improve the quality of life of FAs or their daily functioning. More research is needed to demonstrate the effects of community matron model on FAs care, the role of ehealth needs to be strongly involved.
Decreased quality of life and treatment satisfaction in patients with latent autoimmune diabetes of the adult
Authors: Granado-Casas Minerva , Martínez-Alonso Montserrat , Alcubierre Nuria , Ramírez-Morros Anna , Hernández Marta , Castelblanco Esmeralda , Torres-Puiggros Joan , Mauricio Didac 
Published: October 2017

Objectives
Our main aim was to assess the quality of life (QoL) and treatment satisfaction (TS) of subjects with LADA (latent autoimmune diabetes of the adult) and compare these measures with those of patients with other diabetes types, i.e., type 1 (T1DM) and type 2 diabetes mellitus (T2DM).

Methods
This was a cross-sectional study with a total of 48 patients with LADA, 297 patients with T2DM and 124 with T1DM. The Audit of Diabetes-Dependent Quality of Life (ADDQoL-19) questionnaire and the Diabetes Treatment Satisfaction Questionnaire (DTSQ) were administered. Relevant clinical variables were also assessed. The data analysis included comparisons between groups and multivariate linear models.

Results
The LADA patients presented lower diabetes-specific QoL (p = 0.045) and average weighted impact scores (p = 0.007) than the T2DM patients. The subgroup of LADA patients with diabetic retinopathy (DR) who were treated with insulin had a lower ADDQoL average weighted impact score than the other diabetic groups. Although the overall measure of TS was not different between the LADA and T2DM (p = 0.389) and T1DM (p = 0.091) groups, the patients with LADA showed a poorer hyperglycemic frequency perception than the T2DM patients (p < 0.001) and an improved frequency of hypoglycemic perception compared with the T1DM patients (p = 0.021).

Conclusions
The current findings suggest a poorer quality of life, especially in terms of DR and insulin treatment, among patients with LADA compared with those with T1DM and T2DM. Hyperglycemia frequency perception was also poorer in the LADA patients than in the T1DM and T2DM patients. Further research with prospective studies and a large number of patients is necessary.
Margin derivation from intrafraction patient motion of multi‐target, single isocentre, brain stereotactic radiosurgery treatments
Authors: Caloz Misael , Tran Sébastien , Gau Max , Romano Edouard , Koutsouvelis Nikolaos , Tsoutsou Pelagia G. 
Published: October 2024

AbstractBackgroundBrain metastases are the most common intracranial malignancy and remain a substantial source of morbidity and mortality in cancer patients. Linear accelerator based stereotactic radiosurgery (SRS) is widely used and is frequently delivered by hypo‐fractionnated volumetric modulated arc therapy using non‐coplanar beams, where geometric accuracy and planning margins are a major concern.PurposeTo give a practical analysis of intrafraction patient motion for multi‐target, single isocentre, brain SRS treatments and to derive adapted GTV‐to‐PTV margins.MethodsData of 154 lesions, spread over 85 fractions from 56 patients treated in our institution with the Varian HyperArc SRS solution was processed. Intrafraction patient motion were recorded using an Optical Surface Monitoring System during irradiation. The present study focuses on small tumor volumes, roughly equal or inferior to 1.5 , and frameless mask‐based immobilization. For each treatment session, a tumor displacement vector matrix was calculated from the patient drifts as a function of time. Data were combined together into a representative treatment scenario and the dosimetric impact of GTV displacement was calculated.ResultsRecommended margins due to patient motion range between 0.3 and 1 mm, depending on the distance tumor‐isocentre, and the desired GTV edge dose coverage. Those values should be added quadratically with other sources of uncertainty, such as mechanical isocentre and kV‐MV misalignment.ConclusionThorough analysis of intrafraction patient motion was performed, the dosimetric impact was calculated for different scenarios, and adequate GTV‐to‐PTV margins were derived. These values vary according to the distance isocentre‐to‐GTV, as well as the desired dose coverage, and should be chosen adequately.
Improving Identification of At‐Risk Behaviors in Adolescents With Rheumatic Disease
Authors: Ciaglia Kristina , Lau May , Jo Chan‐hee , Nassi Lorien 
Published: October 2024

ObjectiveMany adolescent patients view their rheumatologist as their primary physician, and therefore it is important to screen youth for sexual activity and substance use as recommended by the American Academy of Pediatrics. We implemented an electronic social history questionnaire (SHQ) and alert system to identify at‐risk behaviors in adolescents with rheumatic disease.MethodsThe SHQ was administered to adolescents 14 years and older with a goal to survey patients’ sexual activity and alcohol, tobacco, and drug use. The SHQ was given via tablet at each rheumatology outpatient visit. A positive response triggered a best practice advisory (BPA) alert when the chart was opened to remind the clinician to discuss these results privately.ResultsA total of 877 unique patients were surveyed. Ninety patients (12%) reported being sexually active, and sexually active patients were significantly older than those who were not (17.2 vs 15 years; P < 0.001). Seventy‐two percent of patients were female, and the mean age was 15.8 years. Sexually active patients were more likely to be smokers, to drink alcohol, and to use other drugs (P < 0.001). Strong associations were observed between alcohol use and male sex (P = 0.0227), White race (P = 0.0052), and public insurance (P = 0.0021).ConclusionOverall, 12% of patients reported being sexually active, underscoring the need to screen adolescents for sexual activity given many rheumatology patients take teratogenic medication. A smaller proportion used substances. Implementing an electronic medical record–based SHQ can help identify patients most at risk, and the BPA serves as a useful tool to remind clinicians to discuss the SHQ privately.
MBene Nanosheets with DNA Adsorbability for Circulating Tumor DNA Assay via Fluorescence Biosensing and Paper‐Based Microfluidic POCT
Authors: Yang Siyi , Zhu Jiajia , Zhao Liangyi , Yang Liyu , Fa Huanbao , Wang Yongzhong , Huo Danqun , Hou Changjun , Zhong Daidi , Yang Mei 
Published: October 2024

AbstractThe detection of circulating tumor DNA (ctDNA) in blood is significant for non‐invasive cancer diagnosis and treatment monitoring. Herein, MBene nanosheets is synthesized and compared with MXene via Density Functional Theory (DFT) calculations and fluorescence kinetic evaluations, for first time, revealing MBene's exceptional DNA adsorbability and discrimination to single‐stranded DNA (ssDNA) and double‐stranded DNA (dsDNA). Then, a sensitive fluorescence biosensor for ctDNA detection is developed, demonstrating impressive performance. To facilitate point‐of‐care testing (POCT) ctDNA, a paper‐based microfluidic chip incorporated a delay area and a mixing channel is designed. The constricted‐expanded structure channel optimization is guided by numerical simulations and experiments. A WeChat mini program named “ctDNA Detection” is designed for readout assay. Furthermore, cell and mice serum samples are analyzed, with Magnetic Bead@Graphene Oxide (MB@GO) and clutch probes for magnetic pre‐enrichment. The results accuracy is confirmed by its consistency with standard qPCR results (AUC = 1). The successful detection of ctDNA in post‐surgery mouse models underscored the biosensor's potential for cancer treatment monitoring. Thus, this research not only advanced the understanding of the MBene‐DNA interaction in biosensing, but also can pave the way for novel applications in bioimaging and nanopore‐based nucleic acid sequencing, leveraging the digital transformation of DNA base‐MBene adsorption differences.
New Strategies for High Efficiency and Precision Bioprinting by DOE Technology and Machine Learning
Authors: Dai Chuyan , Sun Yazhou , Zhang Hangqi , Yuan Zikai , Zhang Bohan , Xie Zhenwei , Li Peixun , Liu Haitao 
Published: October 2024

AbstractExtrusion‐based 3D printing technology is currently demonstrating considerable potential in the field of tissue engineering scaffolds, enabling the construction of in vitro models with complex structures and functions using a wide range of biomaterials and cells at a low cost. In recent years, researchers have spent considerable effort developing novel bio‐inks and employing a greater variety of cell sources to enhance biological compatibility and functionality. However, the majority of current bio‐ink materials are unprintable due to their low viscosity and long curing time, as well as insufficient shape fidelity before the secondary cross‐linking process. The study aims to bridge this gap by optimizing the material ratios and predicting the printing process before work. This article presents new strategies for the design, fabrication, and analysis of a new composite bio‐ink material. The optimal ink ratios are verified by a design of experiments (DOE) experimental design and evaluation metrics for printing printability (Pr) values. A machine learning model is used to predict the ink printing area and determine the printing process parameters. The influence mechanism of ink materials with different concentrations of poly (ethylene glycol) diacrylate (PEGDA) ratios on printed fibers is investigated. Finally, the optimal results are used as an example to demonstrate the printability of multilayer stents. Thus, the design approach allows for the rapid and cost‐effective exploration of novel ink ratios, while also providing higher fidelity and more accurate process metrics for the fabrication of tissue structures with multidimensional variables.
Multidimensional Manipulation of Circular Polarization Airy Beam in Microwave Band‐Based on an Anisotropic Holographic Impedance Metasurface
Authors: Zhang Song , He Sitong , Zhao Shihao , Xue Hao , Han Jiaqi , Liu Haixia , Li Long 
Published: October 2024

AbstractNon‐diffraction Airy beams with self‐accelerating properties have many potential applications in the microwave band, such as providing a viable solution to bypass obstacles for microwave‐based wireless power transfer (WPT) and communication. However, most of the recent work on Airy beams exhibits singular functionality and operates solely in linear polarization, with complex generation setups, which will constrain the applications of the beam in the microwave band. In this paper, based on the anisotropic holographic impedance metasurface (AHIM) with low complexity, a new method is proposed to manipulate Airy beams in multiple dimensions, including circularly polarization state, number of beams, direction of acceleration, and launch position of beam. The underlying principle is the combination of plane coordinate transformation, anisotropy of AHIM, and impedance superposition theory. Further, to validate the proposed method, an AHIM prototype for simultaneously generating two Airy beams with orthogonal circular polarization, different directions of acceleration, and different spatial distribution is designed, simulated, and measured. The proposed method based on AHIM for manipulating the Airy beams multidimensionally in the microwave band offers an efficient strategy to control the energy coverage and polarization state of the beam in different WPT scenarios.
Robust Ultrablack Coating for Omnidirectional Broadband Light Absorption
Authors: Wang Xiao , Wang Yalan , Xiao Youhua , Li Chaolong , Piao Mingxing , Shi Haofei 
Published: October 2024

AbstractDeveloping robust ultrahigh light absorption coatings has been a significant challenge, limited by the absorption‐adhesion tradeoff. Herein, a robust carbon‐based coating with omnidirectional broadband high absorption is designed and fabricated using a one‐step spray method. A novel rapidly cross‐linking resin is utilized as the binder to enhance adhesion. Beyond leveraging the intrinsic optical properties of carbon absorbents, the construction of a micro‐nano multi‐scale trapping structure is crucial for achieving high absorption efficiency in the coating. Innovatively, an inorganic matting agent is introduced to further diminish reflection by scattering incident light. The resulting ultrablack coating demonstrates good adhesion and an exceptionally high integral light absorption of 99.34% within the wavelength range of 1500–1800 nm, marking the highest reported value for binder‐incorporated spray coatings to date. This robust coating also exhibits outstanding thermal stability and resistance to vacuum thermal cycling and vibration. Furthermore, the application of the ultrablack coating via spraying is scalable and can be readily adapted to the surfaces or interiors of complex structural components made from diverse materials. The coating shows remarkable potential for solar energy harvesting and target stealth. This study presents a simple and efficient approach for developing robust ultrablack coatings suitable for widespread practical applications.
Mass Transportation Facilitated Porous Fe/Co Dual‐Site Catalytic Cathodes for Ultrahigh‐Power‐Density Al–Air Fuel Cells
Authors: Chen Kai , Liang Yiqi , Pan Duo , Huang Junheng , Gao Jiyuan , Lu Zhiwen , Liu Xi , Chen Junxiang , Zhang Hao , Hu Xiang , Wen Zhenhai 
Published: October 2024

AbstractThe development of oxygen‐dependent fuel cells is frequently hindered by the high cost of cathode materials and the sluggish kinetics of the oxygen reduction reaction (ORR). It is thus crucial to explore low‐cost and high‐activity catalysts with accelerated mass transport. Here, a high‐throughput screening method is developed by integrating an explicit solvation model with machine learning potential‐based molecular dynamics simulations, which enables the efficient evaluation of a vast array of diverse diatomic combinations and ultimately identifying Fe–Co dual‐atomic sites as the optimal ORR electrocatalysts. The superior electrocatalytic performance of the diatomic Fe/Co sites loaded on 3D‐interconnected ordered macroporous carbon (FeCo‐3DMNC) is experimentally verified, achieving high ORR activity with half‐wave potentials of 0.806 V in acidic and 0.905 V in alkaline environments. Additionally, innovative hybrid acid/alkali aluminum–air fuel cells (hA/A‐AAFCs) incorporating FeCo‐3DMNC as the cathode catalyst demonstrate a notably high open‐circuit voltage of 2.72 V and a record‐breaking power density of 827 mW cm−2, significantly outperforming conventional alkaline aluminum–air fuel cells. This work marks a significant advancement by combining cutting‐edge computational screening with rigorous experimental validation to develop promising electrocatalysts, potentially paving the way for the advanced energy storage and conversion technologies.
Temperature‐controlled radiofrequency treatment of the nasal valve in nasal airway obstruction patients with septal deviation
Authors: Tang Dennis M. , Chen Philip G. , Wu Arthur W. , Yao William C. 
Published: October 2024

Key Points
Nasal valve treatment with TCRF results in NAO symptom improvement in the presence of septal deviation.
Severe septal deviation does not impact the beneficial effect of TCRF nasal valve treatment.
Symptoms improve with TCRF nasal valve treatment even if septal deviations involve the nasal valve.