bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kajian molekular docking, farmakokinetik dan toksisitas tanaman pegagan (Centella asiatica L.) terhadap target terapi antidepresan
Authors: Sifaiya Laily , Hasan Rahmawaty , Choirunniza Aqidatun Naffiah 
Published: September 2024

ABSTRAK
Depresi merupakan penyakit gangguan mental yang kejadiannya di Indonesia terus meningkat setiap tahun dan belum ditemukan obat spesifik untuk pengobatannya. Penelitian pengembangan obat antidepresi dari pegagan perlu dilakukan untuk menganalisis adanya interaksi dari senyawa kimia dalam tanaman pegagan terhadap target reseptor depresi piperidine E20 (PDB ID: 4EY7) dari asetilkolinesterase secara in silico. Senyawa kimia dari tanaman pegagan telah dilakukan skrining berdasarkan aktivitas masing-masing senyawa menggunakan webform Way2drug dan Superpred, dengan hasil diperoleh tiga senyawa yang dipilih berdasarkan perolehan nilai ΔG terendah untuk dilanjutkan pada tahap docking molecular menggunakan Autodock Tools 4.2. Tiga senyawa terbaik diambil dari masing-masing makromolekul yang telah divisualisasikan menggunakan Discovery Studio, kemudian diteruskan dengan prediksi farmakokinetik dan drug likeness menggunakan webform SwissADME, serta prediksi toksisitas menggunakan Toxtree. Senyawa terbaik ditunjukkan dengan senyawa yang memiliki absorbsi yang baik dalam saluran gastrointestinal, tidak terdistribusi dalam jaringan otak dan termasuk pada bagian P-gp substrat, serta tidak berperan sebagai inhibitor dari enzim sitokrom P450. Sedangkan terkait prediksi toksisitasnya, senyawa terbaik ditunjukkan pada senyawa yang tidak memiliki potensi sebagai senyawa karsinogenik, genotoksik dan non genotoksik. Hasil uji yang didapat menetapkan bahwa senyawa yang dapat dijadikan sebagai calon kandidat obat baru antidepresi adalah senyawa madasiatic acid.

Kata Kunci: Antidepresan, Centella asiatica, Farmakokinetik, In Silico, Toksisitas
Al-Tasawuf wa Azmah al-Mustadāmah: Muqārabah Muta’addidah al-Takhaṣṣuṣāt Li Idārah al-Nufāyāt fī Indonesia Mustawḥāt min Mafhūm al-Khilāfah fī Rasāil al-Nūr
Authors: Mudin Moh. Isom , Huringiin Nabila , Ouannes Mohamed Hedi , Türkoğlu Tuğba 
Published: September 2024

This research explores the relationship between the concept of Khilafah in the Risale-i Nur and the "Zero Waste" movement in Indonesia. The study utilizes a literature review, including a compilation of Risale-I Nur, data from the Zero Waste program in Indonesia's National Waste Management Information System (SIPSN), and related literature. This data is then qualitatively analyzed and subjected to content analysis to describe the relevance and implications of it concept on the Indonesia Zero Waste movement. The research demonstrates that the concept of Khilafah transcends the anthropocentric and ecocentric perspectives, emphasizing human responsibility towards the environment. The research further illustrates how the concept of Khilafah utilizes the "Law of Purification" as a foundation for the "Zero Waste" concept. Despite the progress made by Indonesia in waste management, significant challenges remain. The research highlights the role of the Khilafah concept in fostering religious awareness and sensitivity within the "Zero Waste" movements. It elucidates that the connection between the Nursian Khilafah and the "Zero Waste" movements constitutes a religious basis and sensitivity that requires continuous attention. The research is expected to provide profound insights into the potential of the Nursian Khilafah in addressing waste problems and supporting the "Zero Waste" movement in Indonesia
Light-dependent modulation of protein localization and function in living bacteria cells
Authors: Xiao Jie , McQuillen Ryan , Yang Xinxing , Bohrer Christopher , McCausland Joshua , Perez Amilcar , Smith Erika , Chareyre Sylvia , Tsui Ho-Ching , Bruce Kevin , Hla Yin , Winkler Malcolm , Goley Erin , Ramamurthi Kumaran (S) 
Published: September 2024

Abstract

Most bacteria lack membrane-enclosed organelles to compartmentalize cellular processes. In lieu of physical compartments, bacterial proteins are often recruited to macromolecular scaffolds at specific subcellular locations to carry out their functions. Consequently, the ability to modulate a protein’s subcellular location with high precision and speed bears the potential to manipulate its corresponding cellular functions. Here we demonstrate that the CRY2/CIB1 system from Arabidopsis thaliana can be used to rapidly direct proteins to different subcellular locations inside live E. coli cells including the nucleoid, the cell pole, membrane, and the midcell division plane. We further show that such light-induced re-localization can be used to rapidly inhibit cytokinesis in actively dividing E. coli cells and that the CRY2/CIBN binding kinetics can be modulated by green light, adding a new dimension of control to the system. Finally, we tested this optogenetic system in three additional bacterial organisms, B. subtilis, C. crescentus and S. pneumoniae, providing important considerations for the applicability of this system in microbiology and bacterial cell biology.
Microwave-powered liquid metal depolymerization for plastic upcycling
Authors: Gao Jianye , Zhao Jun , Xing Zerong , Guo Minghui , Xie Haijiao , Ma Wangjing , Liu Jing 
Published: September 2024

Abstract

Upcycling waste plastics is highly promising to tackle the global white pollution while achieving sustainable development. However, prevailing approaches often encounter challenges in scalable engineering practices due to either insufficient capability or arduousness in separation, recovery, and purification of catalysts, which inevitably augments the cost of plastic upcycling. Here, we present the microwave-powered liquid metal synergetic depolymerization to facilitate low-cost plastic upcycling. By leveraging the fluidity of liquid metals and their exceptional chemical-bond activation ability under microwave field, this method efficiently converts various polyolefins into narrowband hydrocarbon oil (Oil yield: 81 wt% for PP, 85.9 wt% for PE) and high-value olefin monomers (C2-4= selectivity: 50% for PE, 65.3% for PP) over 30 successive cycles, resulting in a high turnover frequency of 2.83 kgPlastic/mLCatalyst. These captivating advantages offered by electromagnetically-powered liquid metals are also supported by their self-separation features, thereby paving the way for large-scale engineering solutions in waste plastic upcycling.
Assessing population genetic structure and diversity and their driving factors in Phoebe zhennan populations
Authors: Peng Jian , Xie Jiaxin , Gu Yunjie , Guo Hongying , Zhang Shuaiying , Huang Xin , Luo Xiandan , Qian Jianghong , Liu Minhao , Wan Xueqin , Chen Lianghua , Huang Xiong , Zhang Fan , He Fang , Zhu Peng , Zhong Yu , Yang Hanbo 
Published: September 2024

Abstract

Background
Phoebe zhennan, commonly known as “golden-thread nanmu,” is one of the most valuable and protection tree species in China. An accurate understanding of the population genetic structure and its environmental factors is of significance for the protection and selection of new P. zhennan varieties.
Results
Sixteen nSSR and six cpSSR markers were used to determine the genetic diversity and population structure of P. zhennan and the effect of environmental factors on the genetic structure. Low inbreeding (F = 0.237) and high genetic diversity (I = 2.294, h = 0.412) were observed in P. zhennan populations. The genetic differentiation was mainly detected within populations (only 28% and 13% of the variance being between populations according to the nSSR and cpSSR datasets). Among them, the HNSZX (h = 0.469) and SCSZZ (I = 1.943) populations exhibited the highest level of genetic diversity, while the HNXXT (h = 0.041) and SCLJS (I = 0.943) populations exhibited the lowest level of genetic diversity. The average genetic differentiation coefficient (Fst) and gene flow (Nm) were 0.022–0.128 and 1.698–11.373, respectively, which indicated a moderate level of genetic differentiation and a high level of gene flow. STRUCTURE, neighbor-joining clustering, and principal coordinate analysis divided 543 individuals into two or three categories based on the nSSR or cpSSR datasets. Four temperature, three precipitation, five chemical, five physical, and one soil texture variable showed significant effects on the genetic structure and distribution of P. zhennan populations. Compared to nSSR, the genetic differentiation among populations based on cpSSR datasets conformed to the geographic isolation model, suggesting that geographic and genetic distances should be considered for further genetic conservation and breeding utilization. The importance of in situ conservation units, such as populations with a high level of genetic diversity, more private alleles, and haplotypes (e.g., population SCGTS, SCYFS, and YNYJX), should be emphasized. Additionally, breeding, along with artificially assisted population regeneration and restoration, should also be carefully planned, taking into account climate and soil properties at the same time.
Conclusions
In conclusion, this study provided genetic background information for the genetic conservation, management, and utilization of P. zhennan.
Mechanisms of Mechanical Property Degradation and Dynamic Response Characteristics of Hybrid Fiber Reinforced Concrete under High-Temperature Conditions
Authors: Tong Gejun , Pang Jianyong , Tang Bin , Huang Jinkun , Sun Jian 
Published: September 2024

Abstract

In the context of building fire scenarios, this paper thoroughly investigates the degradation patterns of mechanical properties, dynamic response characteristics, and microstructural evolution of hybrid fiber reinforced concrete (HFRC) under high-temperature conditions. A series of orthogonal design experiments were conducted to quantitatively explore the regulatory effects of rice husk ash content, polypropylene fiber, and steel fiber volume fractions on the mechanical properties of HFRC. Using the Split Hopkinson Pressure Bar (SHPB) technique, the dynamic mechanical behavior of HFRC and ordinary concrete (OC) under various temperature gradients was examined, revealing the interactive influence mechanisms of temperature and strain rate on the dynamic mechanical properties of HFRC. Scanning Electron Microscopy (SEM) was utilized to analyze the microstructure of OC and HFRC samples subjected to high-temperature treatment, elucidating the micro-damage mechanisms behind the mechanical property degradation of HFRC in high-temperature environments. The results indicate that the steel fiber content predominantly determines the compressive and tensile strengths of HFRC, while polypropylene fiber plays a crucial role in enhancing the tensile performance of HFRC. Optimal mechanical performance was achieved with 12% rice husk ash content, 0.1% polypropylene fiber volume fraction, and 0.5% steel fiber volume fraction, resulting in a 10.41% and 50.22% increase in compressive and tensile strengths, respectively. Under high-temperature conditions, HFRC exhibited significantly superior mechanical properties compared to OC, particularly in terms of dynamic response characteristics. As the temperature increased, the dynamic compressive strength, dynamic increase factor, and peak toughness of HFRC initially decreased and then increased, consistently maintaining levels higher than those of OC. The study highlights the critical importance of the interaction between temperature and strain rate on the high-temperature dynamic response characteristics of HFRC, and the significant deteriorative impact of elevated temperatures on its microstructure. This research provides a solid scientific basis for enhancing the disaster resistance of concrete structures in fire environments and offers theoretical support for the effective application of hybrid fiber reinforced concrete in practical engineering.
Seismic response of few realistic typical asymmetric buildings
Authors: CHOWDHURY KRISHNENDU , Chowdhury Utsha 
Published: September 2024

Abstract

Asymmetrical designs have been demonstrated to be extremely hazardous in previous earthquakes. One possible cause of the susceptibility might be a disparity of mass or stiffness. The majority of the investigation was devoted to examining the eccentricity that results from an inconsistent distribution of stiffness. Conversely, the systems exhibiting mass eccentricity as a consequence of the unequal mass distribution at the floor level receive comparatively less awareness. Therefore, stiffness along with mass eccentric systems are jointly thoroughly examined in the current research owing to bidirectional components of ground motion that expose more accurately simulating the actual scenario. In order to provide a relatively precise forecast regarding column elements, the explanation of hysteresis behavior takes into account the impact of biaxial interaction caused by synchronous bidirectional movement. The vulnerability of asymmetric structures to stiffness or mass eccentricity has mostly been studied in idealized single-story systems with different levels of eccentricity. In addition, the subject matter of the research has expanded to cover three-story asymmetric systems in order to examine the impact of higher modes. This study investigates a three-story system with larger eccentricity in the first story for functional reasons and lower eccentricities in the upper stories for a more realistic scenario. The current study demonstrates that stiffness eccentric systems and mass eccentric systems are both susceptible to earthquakes. In addition, three-story systems have a significantly higher reaction than their single-story equivalents. This research could enhance design recommendations by providing a greater comprehension of the behavior of plan asymmetric systems.
Adsorptive and Photocatalytic Degradation of Imidacloprid Pesticide from Wastewater via the ZrP/NiSe 2 nanocomposite
Authors: Izadi Mina , Bahrami Kiumars , Targhan Homa 
Published: September 2024

Abstract

In this study, ZrP/NiSe2 nanocomposite was constructed for the first time. Characterizations were carried out by FT-IR, XRD, EDX mapping and FESEM, confirming the nanocomposite formation. The resulting sustainable ZrP/NiSe2 nanocomposite exhibit improved photocatalytic activity in the visible area to remove imidacloprid from water compared to pure ZrP and NiSe2 nanoparticles. Improving the performance of this nanocomposite can be the result of the integration of ZrP and NiSe2 at the nanoscale and the synergistic enhancement of their activity. Furthermore, ZrP/NiSe2 and ZrP can remove significant percentages of imidacloprid from aqueous solutions through adsorption. This result can be explained by the high adsorption affinity of ZrP toward organic pollutants with protonable groups.
Case report: Recurrent enteritis and intestinal obstruction in a patient with chronic mucocutaneous candidiasis due to STAT1 Gain-of-function mutation
Authors: LUO Mingfen , Huang Huan , Nie Hanhui , Liu Yinghui , Chen Yangxia , Zheng Fuying , Xi Liyan , Liu Hongfang 
Published: September 2024

Abstract

We presented a case of chronic mucocutaneous candidiasis (CMC) due to STAT1 GOF mutation with recurrent enteritis and intestinal obstruction. A 33-year-old woman complained of recurrent oral erosion and finger (toe) nails damage for over 30 years. Candida albicans were cultured from the oral mucosa and nails. Sanger sequencing revealed a gain-of-function mutation in STAT1 (c.A1159 G, p.T387A). Since the age of 37, she developed recurrent enteritis and intestinal obstruction. Laboratory examinations revealed an increased pSTAT1 protein expression and a decreased proportion of Th17 cells in peripheral blood lymphocyte (PBMC), with a high expression of pSTAT1 and a scarce expression of IL17A observed in intestinal immunohistochemistry. Intestinal obstruction had not previously been reported as the main clinical manifestation in STAT1 GOF patients. We speculated that the low levels of IL17A impaired the intestinal barrier, which might lead to gastrointestinal disorders in this patient. This case expanded the clinical phenotype of heterozygous STAT1 GOF patients.
The Therapeutic Efficacy of MS473, a Fully Human Single-Chain Variable Fragment Specific to Staphylococcus aureus Toxic Shock Syndrome Toxin-1, in a Mouse Model of Toxic Shock
Authors: Jamnani Fatemeh Rahimi , Moradi Hamid Reza , Soezi Mahdieh , Azizi Masoumeh , Nazari Farzaneh , Fateh Abolfazl , Siadat Seyed Davar 
Published: September 2024

Abstract

Toxic shock syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus, is one of the most potent superantigens involved in causing life-threatening toxic shock syndrome (TSS) and contributes to the onset of some autoimmune diseases. To this end, we have previously identified a fully human single-chain variable fragment antibody (scFv), MS473, exhibiting high binding affinity and specificity for TSST-1 and demonstrating in vitro neutralization activity. In the present study, the therapeutic activity of MS473 was assessed in a D-galactosamine-sensitized mouse model of lethal shock. D-galactosamine-sensitized mice were injected with TSST-1 and then received a single dose of MS473 intraperitoneally (15 mg/kg) after five minutes or intravenously (3 mg/kg) after 10 minutes. The survival rate was examined for seven days. Furthermore, mice were killed after 24 hours, and their kidneys and liver were histopathologically examined. The findings demonstrated a 100% survival rate and no significant pathology damage on tissue samples in both treated groups, administered with MS473 in two different routes compared to the control groups, including toxin-injected mice receiving normal saline or an unrelated scFv. Targeting disseminated TSST-1 with scFvs, which have appropriate permeability and distribution throughout the body, may be an effective way to alleviate the malfunctioning of the immune system caused by TSST-1.
Genetic remodeling of soil diazotrophs enables partial replacement of synthetic nitrogen fertilizer with biological nitrogen fixation in maize
Authors: Martinez-Feria Rafael , Simmonds Maegen B , Ozaydin Bilge , Lewis Stacey , Schwartz Allison , Pluchino Alex , McKellar Megan , Gottlieb Shayin S. , Kayatsky Tasha , Vital Richelle , Mehlman Sharon E. , Caron Zoe , Colaianni Nicholas R. , Ané Jean-Michel , Maeda Junko , Infante Valentina , Karlsson Bjorn H , McLimans Caitlin , Vyn Tony , Hanson Brendan , Verhagen Garrett , Nevins Clayton , Reese Lori , Otyama Paul , Robinson Alice , Learmonth Timothy , Miller Christine , Havens Keira , Tamsir Alvin , Temme Karsten 
Published: September 2024

Abstract

Increasing biological nitrogen (N) fixation (BNF) in maize production could reduce the environmental impacts of N fertilizer use, but reactive N in the rhizosphere of maize limits the BNF process. Using non-transgenic methods, we developed gene-edited strains of Klebsiella variicola (Kv137-2253) and Kosakonia sacchari (Ks6-5687) bacteria optimized for root-associated BNF and ammonium excretion in N-rich conditions. We present evidence from in vitro, in planta and field experiments that confirms that our genetic remodeling strategy derepresses BNF activity in N-rich systems and increases ammonium excretion by orders of magnitude above the respective wildtype strains. BNF is demonstrated in controlled environments by the transfer of labeled 15N2 gas from the rhizosphere to the chlorophyll of inoculated maize plants. This was corroborated in several 15N isotope tracer field experiments where inoculation with the formulated, commercial-grade product derived from the gene-edited strains (PIVOT BIO PROVEN® 40) provided on average 21 kg N ha− 1 to the plant by the VT-R1 growth stages. Data from small-plot and on-farm trials suggest that this technology can improve crop N status pre-flowering and has potential to mitigate the risk of yield loss associated with a reduction in synthetic N fertilizer inputs.
Development of an Industrial-Size Aeroponic Rooting System and Plant Cutting Rooting Studies
Authors: KARADAĞ Hakan , Polatcı Hakan , Saraçoğlu Onur , Cömert Mehmet , Vural Güzella Yılmaz , Yıldız Kenan 
Published: September 2024

Abstract

Aeroponic agriculture is an innovative system that allows plants to grow and develop by fogging and applying water directly to the bare root area. Thus, oxygen and water, which are the limiting factors of traditional agriculture, can be supplied at sufficient levels in this system. Within the scope of the study, an industrial-sized aeroponic rooting unit was designed and manufactured. After drawings were made in the digital environment, the manufacturing process started. The unit consists of 3 lines, each with 3 floors. Nine cabins were produced, each cabin measuring 2x0.4x1 m. The control unit is designed to independently control each cabin's humidity, temperature, lighting, and ventilation features. In the study, research and findings on the potential of aeroponic agriculture were examined, focusing primarily on the effects of the aeroponic system on the growth of plant roots and its performance on various plant species. Within the scope of the study, the rooting performances of black mulberry (Morus nigra) and Judas tree (Cercis siliquastrum) cuttings, which are very difficult to root with traditional methods, were examined in perlite and aeroponic environments. The effect of cutting time, growth regulator doses, and various parameters on root formation and development during the rooting process were examined. Applying 6000 ppm IBA on black mulberry wood cuttings in the aeroponic system reached the highest rooting rate (85.19%) and callus formation rate (96.30%). The rooting rate of black mulberry wood cuttings in perlite medium with 6000 ppm IBA application was 59.26%. Application of 4000 ppm IBA on black mulberry green cuttings in the aeroponic system reached the highest callus formation rate (92.59%) and rooting rate (81.48%). These values ​​show a significant increase compared to the control group. At the same time, the highest rooting rate (74.08%) in perlite medium was again obtained with 4000 ppm IBA application. Application of 6000 ppm IBA to Judas tree green cuttings in the aeroponic system reached the highest rooting rate (29.63%) and callus formation rate (25.92%). It has been observed that the aeroponic system is more successful in terms of rooting rates and number of roots compared to perlite media.