bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ferns, mosses and liverworts as model systems for light‐mediated chloroplast movements
Authors: Suetsugu Noriyuki , Higa Takeshi , Wada Masamitsu 
Published: November 2016

AbstractLight‐induced chloroplast movement is found in most plant species, including algae and land plants. In land plants with multiple small chloroplasts, under weak light conditions, the chloroplasts move towards the light and accumulate on the periclinal cell walls to efficiently perceive light for photosynthesis (the accumulation response). Under strong light conditions, chloroplasts escape from light to avoid photodamage (the avoidance response). In most plant species, blue light induces chloroplast movement, and phototropin receptor kinases are the blue light receptors. Molecular mechanisms for photoreceptors, signal transduction and chloroplast motility systems are being studied using the model plant Arabidopsis thaliana. However, to further understand the molecular mechanisms and evolutionary history of chloroplast movement in green plants, analyses using other plant systems are required. Here, we review recent works on chloroplast movement in green algae, liverwort, mosses and ferns that provide new insights on chloroplast movement.
Fusarium mangiferae localization in planta during initiation and development of mango malformation disease
Authors: Cohen Y. , Belausov E. , Maymon M. , Elazar M. , Shulman I. , Saada D. , Shtienberg D. , Freeman S. 
Published: November 2016

Mango malformation disease (MMD), caused by Fusarium mangiferae, is a major constraint to mango production, causing significant yield reduction resulting in severe economic impact. The present study characterizes fungal localization in planta during initiation and development of vegetative and floral malformation. Young mango trees were artificially inoculated with a green fluorescent protein (GFP)‐expressing strain of F. mangiferae. Shoots and buds were sampled periodically over a period of more than a year and localization of the GFP‐expressing fungi was determined using confocal microscopy. Fungal localization appears to be epiphytic: mycelia remained in close contact with the plant surface but did not penetrate the tissue. In vegetative malformation and in young inflorescences, the fungus was confined to protected regions between scales, young leaf bases and buds. Fungal colonization was only very rarely detected on open leaves or on exposed shoot sections. In developed flowers, mycelia were localized mainly to protected regions at the base of the flower organs. Upon development of the inner flower organs, specific mycelial growth occurred around the anthers and the style. Mycelial penetration through the stylar tract into aborting carpels was observed. For several months, mycelia were confined to the surface of the organs and were not detected within plant tissues. Only at later stages, transient saprophytic growth of the fungus was detected causing the malformed inflorescences to senesce and collapse, concurrent with dispersion of conidia. Implications of the present study on MMD in natural field infections are discussed.
Movers and Stayers in The Farming Sector: Accounting for Unobserved Heterogeneity in Structural Change
Authors: Saint-Cyr Legrand D. F. , Piet Laurent 
Published: November 2016

SummaryThe paper investigates whether accounting for unobserved heterogeneity in farms' size transition processes improves the representation of structural change in agriculture. Considering a mixture of two types of farm, the mover–stayer model is applied for the first time in an agricultural economics context. The maximum likelihood method and the expectation–maximization algorithm are used to estimate the model's parameters. An empirical application to a panel of French farms from 2000 to 2013 shows that the mover–stayer model outperforms the homogeneous Markov chain model in recovering the transition process and predicting the future distribution of farm sizes.
A New Strategy for Diagnostic Model Assessment in Capture–Recapture
Authors: McCrea Rachel S. , Morgan Byron J. T. , Gimenez Olivier 
Published: November 2016

SummaryCommon to both diagnostic tests used in capture–recapture and score tests is the idea that starting from a simple base model it is possible to interrogate data to determine whether more complex parameter structures will be supported. Current recommendations advise that diagnostic tests are performed as a precursor to a model selection step. We show that certain well-known diagnostic tests for examining the fit of capture–recapture models to data are in fact score tests. Because of this direct relationship we investigate a new strategy for model assessment which combines the diagnosis of departure from basic model assumptions with a step-up model selection, all based on score tests. We investigate the power of such an approach to detect common reasons for lack of model fit and compare the performance of this new strategy with the existing recommendations by using simulation. We present motivating examples with real data for which the extra flexibility of score tests results in an improved performance compared with diagnostic tests.
Curvature determination in the bending test of continuous fibre reinforcements
Authors: Liang B. , Chaudet P. , Boisse P. 
Published: November 2016

AbstractBending deformation is one of the main deformation modes in the forming of continuous fibre reinforcements. It is very specific compared to the classical continuous materials. In most textile material forming simulation models, bending stiffness is neglected, but taking into account it would give a more accurate predication, particularly the shape of wrinkles. In the deformation, fibres constituting the reinforcement can slide to each other, resulting in the bending stiffness of reinforcement is not directly related to its in‐plane tensile modulus as the classical continuous materials. Consequently, it is necessary to determine the bending stiffness by the experimental method. A cantilever bending stiffness test approach was proposed to measure the bending stiffness of continuous fibre reinforcements. A CCD camera was used to take the bending deflection shape. To calculate the curvature of bending deflection curve, uniform quartic B‐spline curve was chosen. A detailed process of curvature calculating of deflection curve was given in this paper. A single bending test gives the bending moment as a function of curvature from zero to the maximum value. Bending tests were conducted to validate the capacity of the proposed approach. A smooth curvature plot along the bending deflection curve was obtained for different types of reinforcements, and the non‐linear bending deformation behaviour can be identified with the proposed bending test method.
Trademark protection, quality improvement and exports in developing countries
Authors: Yang Lei , Yin Xiaopeng , He Yin 
Published: November 2016

AbstractTrademarks indicate the inherent quality or other distinguishing features of products. Strong trademark protection can lower consumers' costs of searching for preferred quality characteristics. With weak trademark protection, firms in developing countries may not be willing to provide high‐quality products. With strong trademark protection, firms have an incentive to maintain or improve quality over time in order not to erode the value of their trademarks. By solving the theoretical model, we show that strengthened trademark protection in developing countries may induce domestic firms to become exporters and raise the quality of products on the export market. The welfare analyses show that strengthened trademark protection in developing countries benefits Southern customers and firms producing high‐quality products, but harms firms producing counterfeits and uninformed customers, if firm H charges low prices. Given firm H charges a high price, the strengthened trademark protection only helps firm H and informed customers in the South while leaves the others unaffected.
Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature‐dependent ATP release from human blood and endothelial cells
Authors: Kalsi Kameljit K. , Chiesa Scott T. , Trangmar Steven J. , Ali Leena , Lotlikar Makrand D. , González‐Alonso José 
Published: November 2016

New Findings
What is the central question of this study?
Skin and muscle blood flow increases with heating and decreases with cooling, but the temperature‐sensitive mechanisms underlying these responses are not fully elucidated.

What is the main finding and its importance?
We found that local tissue hyperaemia was related to elevations in ATP release from erythrocytes. Increasing intravascular ATP augmented skin and tissue perfusion to levels equal or above thermal hyperaemia. ATP release from isolated erythrocytes was altered by heating and cooling. Our findings suggest that erythrocytes are involved in thermal regulation of blood flow via modulation of ATP release.

Local tissue perfusion changes with alterations in temperature during heating and cooling, but the thermosensitivity of the vascular ATP signalling mechanisms for control of blood flow during thermal interventions remains unknown. Here, we tested the hypotheses that the release of the vasodilator mediator ATP from human erythrocytes, but not from endothelial cells or other blood constituents, is sensitive to both increases and reductions in temperature and that increasing intravascular ATP availability with ATP infusion would potentiate thermal hyperaemia in limb tissues. We first measured blood temperature, brachial artery blood flow and plasma [ATP] during passive arm heating and cooling in healthy men and found that they increased by 3.0 ± 1.2°C, 105 ± 25 ml min−1 °C−1 and twofold, respectively, (all P < 0.05) with heating, but decreased or remained unchanged with cooling. In additional men, infusion of ATP into the brachial artery increased skin and deep tissue perfusion to levels equal or above thermal hyperaemia. In isolated erythrocyte samples exposed to different temperatures, ATP release increased 1.9‐fold from 33 to 39°C (P < 0.05) and declined by ∼50% at 20°C (P < 0.05), but no changes were observed in cultured human endothelial cells, plasma or serum samples. In conclusion, increases in plasma [ATP] and skin and deep tissue perfusion with limb heating are associated with elevations in ATP release from erythrocytes, but not from endothelial cells or other blood constituents. Erythrocyte ATP release is also sensitive to temperature reductions, suggesting that erythrocytes may function as thermal sensors and ATP signalling generators for control of tissue perfusion during thermal interventions.
Human motoneurone excitability is depressed by activation of serotonin 1A receptors with buspirone
Authors: D'Amico Jessica M. , Butler Annie A. , Héroux Martin E. , Cotel Florence , Perrier Jean‐François M. , Butler Jane E. , Gandevia Simon C. , Taylor Janet L. 
Published: November 2016

Key points
In the adult turtle spinal cord, action potential generation in motoneurones is inhibited by spillover of serotonin to extrasynaptic serotonin 1A (5‐HT1A) receptors at the axon initial segment. We explored whether ingestion of the 5‐HT1A receptor partial agonist, buspirone, decreases motoneurone excitability in humans.
Following ingestion of buspirone, two tests of motoneurone excitability showed decreases. F‐wave areas and persistence in an intrinsic muscle of the hand were reduced, as was the area of cervicomedullary motor evoked potentials in biceps brachii.
Our findings suggest that activation of 5‐HT1A receptors depresses human motoneurone excitability. Such a depression could contribute to decreased motoneurone output during fatiguing exercise if there is high serotonergic drive to the motoneurones.
AbstractIntense serotonergic drive in the turtle spinal cord results in serotonin spillover to the axon initial segment of the motoneurones where it activates serotonin 1A (5‐HT1A) receptors and inhibits generation of action potentials. We examined whether activation of 5‐HT1A receptors decreases motoneurone excitability in humans by determining the effects of a 5‐HT1A receptor partial agonist, buspirone, on F waves and cervicomedullary motor evoked potentials (CMEPs). In a placebo‐controlled double‐blind study, 10 participants were tested on two occasions where either placebo or 20 mg of buspirone was administered orally. The ulnar nerve was stimulated supramaximally to evoke F waves in abductor digiti minimi (ADM). CMEPs and the maximal M wave were elicited in biceps brachii by cervicomedullary stimulation and brachial plexus stimulation, respectively. Following buspirone intake, F‐wave area and persistence, as well as CMEP area, were significantly decreased. The mean post‐pill difference in normalized F‐wave areas and persistence between buspirone and placebo days was –27% (–42, –12; 95% confidence interval) and –9% (–16, –2), respectively. The mean post‐pill difference in normalized CMEP area between buspirone and placebo days showed greater variation and was –31% (–60, –2). In conclusion, buspirone reduces motoneurone excitability in humans probably via activation of 5‐HT1A receptors at the axon initial segment. This has implications for motor output during high drive to the motoneurones when serotonin may spill over to these inhibitory receptors and consequently inhibit motoneurone output. Such a mechanism could potentially contribute to fatigue with exercise.
Improving photosynthesis and crop productivity by accelerating recovery from photoprotection
Authors: Kromdijk Johannes , Głowacka Katarzyna , Leonelli Lauriebeth , Gabilly Stéphane T. , Iwai Masakazu , Niyogi Krishna K. , Long Stephen P. 
Published: November 2016

Faster light adaptation improves productivityCrop plants protect themselves from excess sunlight by dissipating some light energy as heat, readjusting their systems when shadier conditions prevail. But the photosynthetic systems do not adapt to fluctuating light conditions as rapidly as a cloud passes overhead, resulting in suboptimal photosynthetic efficiency. Kromdijket al.sped up the adaptation process by accelerating interconversion of violaxanthin and zeaxanthin in the xanthophyll cycle and by increasing amounts of a photosystem II subunit. Tobacco plants tested with this system showed about 15% greater plant biomass production in natural field conditions.Science, this issue p.857
Visualizing the Essential Role of Complete Virion Assembly Machinery in Efficient Hepatitis C Virus Cell-to-Cell Transmission by a Viral Infection-Activated Split-Intein-Mediated Reporter System
Authors: Zhao Fanfan , Zhao Ting , Deng Libin , Lv Dawei , Zhang Xiaolong , Pan Xiaoyu , Xu Jun , Long Gang 
Published: November 2016

ABSTRACT
Hepatitis C virus (HCV) infects 2 to 3% of the world population and is a leading cause of liver diseases such as fibrosis, cirrhosis, and hepatocellular carcinoma. Many aspects of HCV study, ranging from molecular virology and antiviral drug development to drug resistance profiling, were supported by straightforward assays of HCV replication and infection. Among these assays, the HCV-dependent fluorescence relocalization (HDFR) system allowed live-cell visualization of infection without modifying the viral genome, but this strategy required careful recognition of the fluorescence relocalization pattern for its high fluorescence background in the cytoplasm. In this study, to achieve background-free visualization of HCV infection, a viral infection-activated split-intein-mediated reporter system (VISI) was devised. Uninfected Huh7.5.1-VISI cells show no background signal, while HCV infection specifically illuminates the nuclei of infected Huh7.5.1-VISI cells with either green fluorescent protein (GFP) or mCherry. Combining VISI-GFP and VISI-mCherry systems, we revisited HCV cell-to-cell transmission with clear-cut distinction of donor and recipient cells in a live-cell manner. Independently of virion assembly, exosomes have been reported to transfer HCV subgenomic RNA to initiate replication in uninfected cells, which suggested an assembly-free pathway. However, our data demonstrated that HCV structural genes and the p7 gene were essential for not only cell-free infectivity but also cell-to-cell transmission. Additionally, depletion of apolipoprotein E (ApoE) from donor cells but not from recipient cells significantly reduced HCV cell-to-cell transmission efficiency. In summary, we developed a background-free cell-based reporter system for convenient live-cell visualization of HCV infection, and our data indicate that complete HCV virion assembly machinery is essential for both cell-free and cell-to-cell transmission.

IMPORTANCE
Hepatitis C virus (HCV) infects hepatocytes via two pathways: cell-free infection and cell-to-cell transmission. Structural modules of the HCV genome are required for production of infectious cell-free virions; however, the role of specific genes within the structural module in cell-to-cell transmission is not clearly defined. Our data demonstrate that deletion of core, E1E2, and p7 genes individually results in no HCV cell-to-cell transmission and that ApoE knockdown from donor cells causes less-efficient cell-to-cell transmission. Thus, this work indicates that the complete HCV assembly machinery is required for HCV cell-to-cell transmission. At last, this work presents an optimized viral infection-activated split-intein-mediated reporter system for easy live-cell monitoring of HCV infection.
Robust Central Nervous System Pathology in Transgenic Mice following Peripheral Injection of α-Synuclein Fibrils
Authors: Ayers Jacob I. , Brooks Mieu M. , Rutherford Nicola J. , Howard Jasie K. , Sorrentino Zachary A. , Riffe Cara J. , Giasson Benoit I. 
Published: November 2016

ABSTRACT

Misfolded α-synuclein (αS) is hypothesized to spread throughout the central nervous system (CNS) by neuronal connectivity leading to widespread pathology. Increasing evidence indicates that it also has the potential to invade the CNS via peripheral nerves in a prion-like manner. On the basis of the effectiveness following peripheral routes of prion administration, we extend our previous studies of CNS neuroinvasion in M83 αS transgenic mice following hind limb muscle (intramuscular [i.m.]) injection of αS fibrils by comparing various peripheral sites of inoculations with different αS protein preparations. Following intravenous injection in the tail veins of homozygous M83 transgenic (M83
+/+
) mice, robust αS pathology was observed in the CNS without the development of motor impairments within the time frame examined. Intraperitoneal (i.p.) injections of αS fibrils in hemizygous M83 transgenic (M83
+/−
) mice resulted in CNS αS pathology associated with paralysis. Interestingly, injection with soluble, nonaggregated αS resulted in paralysis and pathology in only a subset of mice, whereas soluble Δ71-82 αS, human βS, and keyhole limpet hemocyanin (KLH) control proteins induced no symptoms or pathology. Intraperitoneal injection of αS fibrils also induced CNS αS pathology in another αS transgenic mouse line (M20), albeit less robustly in these mice. In comparison, i.m. injection of αS fibrils was more efficient in inducing CNS αS pathology in M83 mice than i.p. or tail vein injections. Furthermore, i.m. injection of soluble, nonaggregated αS in M83
+/−
mice also induced paralysis and CNS αS pathology, although less efficiently. These results further demonstrate the prion-like characteristics of αS and reveal its efficiency to invade the CNS via multiple routes of peripheral administration.

IMPORTANCE
The misfolding and accumulation of α-synuclein (αS) inclusions are found in a number of neurodegenerative disorders and is a hallmark feature of Parkinson's disease (PD) and PD-related diseases. Similar characteristics have been observed between the infectious prion protein and αS, including its ability to spread from the peripheral nervous system and along neuroanatomical tracts within the central nervous system. In this study, we extend our previous results and investigate the efficiency of intravenous (i.v.), intraperitoneal (i.p.), and intramuscular (i.m.) routes of injection of αS fibrils and other protein controls. Our data reveal that injection of αS fibrils via these peripheral routes in αS-overexpressing mice are capable of inducing a robust αS pathology and in some cases cause paralysis. Furthermore, soluble, nonaggregated αS also induced αS pathology, albeit with much less efficiency. These findings further support and extend the idea of αS neuroinvasion from peripheral exposures.
Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export
Authors: Behrens Ryan T. , Aligeti Mounavya , Pocock Ginger M. , Higgins Christina A. , Sherer Nathan M. 
Published: November 2016

ABSTRACT
HIV-1's Rev protein forms a homo-oligomeric adaptor complex linking viral RNAs to the cellular CRM1/Ran-GTP nuclear export machinery through the activity of Rev's prototypical leucine-rich nuclear export signal (NES). In this study, we used a functional fluorescently tagged Rev fusion protein as a platform to study the effects of modulating Rev NES identity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and infectious virion production. We found that Rev activity was remarkably tolerant of diverse NES sequences, including supraphysiological NES (SNES) peptides that otherwise arrest CRM1 transport complexes at nuclear pores. Rev's ability to tolerate a SNES was both position and multimerization dependent, an observation consistent with a model wherein Rev self-association acts to transiently mask the NES peptide(s), thereby biasing Rev's trafficking into the nucleus. Combined imaging and functional assays also indicated that NES masking underpins Rev's well-known tendency to accumulate at the nucleolus, as well as Rev's capacity to activate optimal levels of late viral gene expression. We propose that Rev multimerization and NES masking regulates Rev's trafficking to and retention within the nucleus even prior to RNA binding.

IMPORTANCE
HIV-1 infects more than 34 million people worldwide causing >1 million deaths per year. Infectious virion production is activated by the essential viral Rev protein that mediates nuclear export of intron-bearing late-stage viral mRNAs. Rev's shuttling into and out of the nucleus is regulated by the antagonistic activities of both a peptide-encoded N-terminal nuclear localization signal and C-terminal nuclear export signal (NES). How Rev and related viral proteins balance strong import and export activities in order to achieve optimal levels of viral gene expression is incompletely understood. We provide evidence that multimerization provides a mechanism by which Rev transiently masks its NES peptide, thereby biasing its trafficking to and retention within the nucleus. Targeted pharmacological disruption of Rev-Rev interactions should perturb multiple Rev activities, both Rev-RNA binding and Rev's trafficking to the nucleus in the first place.