bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fenugreek supplementation during high-fat feeding improves specific markers of metabolic health
Authors: Knott Eric J. , Richard Allison J. , Mynatt Randall L. , Ribnicky David , Stephens Jacqueline M. , Bruce-Keller Annadora 
Published: October 2017

AbstractTo assess the metabolically beneficial effects of fenugreek (Trigonella foenum-graecum), C57BL/6J mice were fed a low- or high-fat diet for 16 weeks with or without 2% (w/w) fenugreek supplementation. Body weight, body composition, energy expenditure, food intake, and insulin/glucose tolerance were measured regularly, and tissues were collected for histological and biochemical analysis after 16 weeks of diet exposure. Fenugreek did not alter body weight, fat mass, or food intake in either group, but did transiently improve glucose tolerance in high fat-fed mice. Fenugreek also significantly improved high-density lipoprotein to low-density lipoprotein ratios in high fat-fed mice without affecting circulating total cholesterol, triglycerides, or glycerol levels. Fenugreek decreased hepatic expression of fatty acid-binding protein 4 and increased subcutaneous inguinal adipose tissue expression of adiponectin, but did not prevent hepatic steatosis. Notably, fenugreek was not as effective at improving glucose tolerance as was four days of voluntary wheel running. Overall, our results demonstrate that fenugreek promotes metabolic resiliency via significant and selected effects on glucose regulation, hyperlipidemia, and adipose pathology; but may not be as effective as behavioral modifications at preventing the adverse metabolic consequences of a high fat diet.
Measuring molecular frequencies in the 1–10 μm range at 11-digits accuracy
Authors: Insero G. , Borri S. , Calonico D. , Pastor P. Cancio , Clivati C. , D’Ambrosio D. , De Natale P. , Inguscio M. , Levi F. , Santambrogio G. 
Published: October 2017

AbstractHigh-resolution spectroscopy in the 1–10 μm region has never been fully tackled for the lack of widely-tunable and practical light sources. Indeed, all solutions proposed thus far suffer from at least one of three issues: they are feasible only in a narrow spectral range; the power available for spectroscopy is limited; the frequency accuracy is poor. Here, we present a setup for high-resolution spectroscopy, whose approach can be applied in the whole 1–10 μm range. It combines the power of quantum cascade lasers (QCLs) and the accuracy achievable by difference frequency generation using an orientation patterned GaP crystal. The frequency is measured against a primary frequency standard using the Italian metrological fibre link network. We demonstrate the performance of the setup by measuring a vibrational transition in a highly-excited metastable state of CO around 6 μm with 11 digits of precision.
Imaging Atomic Scale Dynamics on III–V Nanowire Surfaces During Electrical Operation
Authors: Webb J. L. , Knutsson J. , Hjort M. , McKibbin S. R. , Lehmann S. , Thelander C. , Dick K. A. , Timm R. , Mikkelsen A. 
Published: October 2017

AbstractAs semiconductor electronics keep shrinking, functionality depends on individual atomic scale surface and interface features that may change as voltages are applied. In this work we demonstrate a novel device platform that allows scanning tunneling microscopy (STM) imaging with atomic scale resolution across a device simultaneously with full electrical operation. The platform presents a significant step forward as it allows STM to be performed everywhere on the device surface and high temperature processing in reactive gases of the complete device. We demonstrate the new method through proof of principle measurements on both InAs and GaAs nanowire devices with variable biases up to 4 V. On InAs nanowires we observe a surprising removal of atomic defects and smoothing of the surface morphology under applied bias, in contrast to the expected increase in defects and electromigration-related failure. As we use only standard fabrication and scanning instrumentation our concept is widely applicable and opens up the possibility of fundamental investigations of device surface reliability as well as new electronic functionality based on restructuring during operation.
Counterbalancing anti-adhesive effects of Tenascin-C through fibronectin expression in endothelial cells
Authors: Radwanska Agata , Grall Dominique , Schaub Sébastien , Divonne Stéphanie Beghelli-de la Forest , Ciais Delphine , Rekima Samah , Rupp Tristan , Sudaka Anne , Orend Gertraud , Van Obberghen-Schilling Ellen 
Published: October 2017

AbstractCellular fibronectin (FN) and tenascin-C (TNC) are prominent development- and disease-associated matrix components with pro- and anti-adhesive activity, respectively. Whereas both are present in the tumour vasculature, their functional interplay on vascular endothelial cells remains unclear. We have previously shown that basally-oriented deposition of a FN matrix restricts motility and promotes junctional stability in cultured endothelial cells and that this effect is tightly coupled to expression of FN. Here we report that TNC induces FN expression in endothelial cells. This effect counteracts the potent anti-adhesive activity of TNC and leads to the assembly of a dense highly-branched subendothelial matrix that enhances tubulogenic activity. These findings suggest that pro-angiogenic remodelling of the perivascular matrix may involve TNC-induced upregulation of FN in endothelial cells.
Autocrine DUSP28 signaling mediates pancreatic cancer malignancy via regulation of PDGF-A
Authors: Lee Jungwhoi , Lee Jungsul , Yun Jeong Hun , Choi Chulhee , Cho Sayeon , Kim Seung Jun , Kim Jae Hoon 
Published: October 2017

AbstractPancreatic cancer remains one of the most deadly cancers with a grave prognosis. Despite continuous efforts to improve remedial values, limited progress has been made. We have reported that dual specificity phosphatase 28 (DUSP28) has a critical role of chemo-resistance and migration in pancreatic cancers. However, its mechanism remains unclear. Here, we further clarify the function of DUSP28 in pancreatic cancers. Analysis using a public microarray database and in vitro assay indicated a critical role of platelet derived growth factor A (PDGF-A) in pancreatic cancer malignancy. PDGF-A was positively regulated by DUSP28 expression at the mRNA and protein levels. Enhanced DUSP28 sensitized pancreatic cancer cells to exogenous PDGF-A treatment in migration, invasion, and proliferation. Transfection with siRNA targeting DUSP28 blunted the influence of administered PDGF-A by inhibition of phosphorylation of FAK, ERK1/2, and p38 signalling pathways. In addition, DUSP28 and PDGF-A formed an acquired autonomous autocrine-signaling pathway. Furthermore, targeting DUSP28 inhibited the tumor growth and migratory features through the blockade of PDGF-A expression and intracellular signaling in vivo. Our results establish novel insight into DUSP28 and PDGF-A related autonomous signaling pathway in pancreatic cancer.
Use of pulmonary CT angiography with low tube voltage and low-iodine-concentration contrast agent to diagnose pulmonary embolism
Authors: Hu Xuemei , Ma Liya , Zhang Jinhua , Li Zhen , Shen Yaqi , Hu Daoyu 
Published: October 2017

AbstractPulmonary CT angiography (CTPA) is regarded as the preferred imaging method in diagnosing pulmonary embolism (PE). Considering the harm of radiation exposure and the side effect of iodinated contrast agent, CTPA protocol with low tube voltage and low dose of contrast agent became research hotspot in last decade. The present study evaluates the image quality, radiation dose, positive rate of PE and the location of PE with a CTPA protocol using low tube voltage (80 kVp) and low-iodine-concentration contrast agent (270 mg I/ml) in patients suspected of PE compared to a conventional CTPA protocol (120 kVp, 350 mg I/ml). The results showed that 80 kVp CTPA protocol with 40 ml 270 mg I/ml achieved equally subjective image quality and a positive rate for diagnosing PE, though the quantitative image quality was reduced compared to the 120 kVp CTPA protocol with 40 ml 350 mg I/ml administered, with a 63.6% decrease in radiation dose and a 22.9% reduction in iodine content of contrast agent. Our results document that CTPA protocol with low tube voltage and low iodine concentration of contrast agent is satisfied to the clinical application.
Determination of a potential quantitative measure of the state of the lung using lung ultrasound spectroscopy
Authors: Demi Libertario , van Hoeve Wim , van Sloun Ruud J. G. , Soldati Gino , Demi Marcello 
Published: October 2017

AbstractB-lines are ultrasound-imaging artifacts, which correlate with several lung-pathologies. However, their understanding and characterization is still largely incomplete. To further study B-lines, lung-phantoms were developed by trapping a layer of microbubbles in tissue-mimicking gel. To simulate the alveolar size reduction typical of various pathologies, 170 and 80 µm bubbles were used for phantom-type 1 and 2, respectively. A normal alveolar diameter is approximately 280 µm. A LA332 linear-array connected to the ULA-OP platform was used for imaging. Standard ultrasound (US) imaging at 4.5 MHz was performed. Subsequently, a multi-frequency approach was used where images were sequentially generated using orthogonal sub-bands centered at different frequencies (3, 4, 5, and 6 MHz). Results show that B-lines appear predominantly with phantom-type 2. Moreover, the multi-frequency approach revealed that the B-lines originate from a specific portion of the US spectrum. These results can give rise to significant clinical applications since, if further confirmed by extensive in-vivo studies, the native frequency of B-lines could provide a quantitative-measure of the state of the lung.
Fractional Laser Releases Tumor-Associated Antigens in Poorly Immunogenic Tumor and Induces Systemic Immunity
Authors: Kawakubo Masayoshi , Cunningham Trevor J. , Demehri Shadmehr , Manstein Dieter 
Published: October 2017

AbstractCurrently ablative fractional photothermolysis (aFP) with CO2 laser is used for a wide variety of dermatological indications. This study presents and discusses the utility of aFP for treating oncological indications. We used a fractional CO2 laser and anti-PD-1 inhibitor to treat a tumor established unilaterally by the CT26 wild type (CT26WT) colon carcinoma cell line. Inoculated tumors grew significantly slower in aFP-treated groups (aFP and aFP + anti-PD-1 groups) and complete remission was observed in the aFP-treated groups. Flow cytometric analysis showed aFP treatment elicited an increase of CD3+, CD4+, CD8+ vand epitope specific CD8+ T cells. Moreover, the ratio of CD8+ T cells to Treg increased in the aFP-treated groups. Additionally, we established a bilateral CT26WT-inoculated mouse model, treating tumors on one-side and observing both tumors. Interestingly, tumors grew significantly slower in the aFP + anti-PD-1 groups and complete remission was observed for tumors on both aFP-treated and untreated sides. This study has demonstrated a potential role of aFP treatments in oncology.
Cyclophilin D ablation is associated with increased end-ischemic mitochondrial hexokinase activity
Authors: Nederlof Rianne , van den Elshout Mark A. M. , Koeman Anneke , Uthman Laween , Koning Iris , Eerbeek Otto , Weber Nina C. , Hollmann Markus W. , Zuurbier Coert J. 
Published: October 2017

AbstractBoth the absence of cyclophilin D (CypD) and the presence of mitochondrial bound hexokinase II (mtHKII) protect the heart against ischemia/reperfusion (I/R) injury. It is unknown whether CypD determines the amount of mtHKII in the heart. We examined whether CypD affects mtHK in normoxic, ischemic and preconditioned isolated mouse hearts. Wild type (WT) and CypD−/− mouse hearts were perfused with glucose only and subjected to 25 min ischemia and reperfusion. At baseline, cytosolic and mtHK was similar between hearts. CypD ablation protected against I/R injury and increased ischemic preconditioning (IPC) effects, without affecting end-ischemic mtHK. When hearts were perfused with glucose, glutamine, pyruvate and lactate, the preparation was more stable and CypD ablation−resulted in more protection that was associated with increased mtHK activity, leaving little room for additional protection by IPC. In conclusion, in glucose only-perfused hearts, deletion of CypD is not associated with end-ischemic mitochondrial-HK binding. In contrast, in the physiologically more relevant multiple-substrate perfusion model, deletion of CypD is associated with an increased mtHK activity, possibly explaining the increased protection against I/R injury.
Statistical analysis and a case study of tropical cyclones that trigger the onset of the South China Sea summer monsoon
Authors: Huangfu Jingliang , Huang Ronghui , Chen Wen 
Published: October 2017

AbstractThis paper addresses whether a tropical cyclone can trigger the onset of the South China Sea (SCS) summer monsoon (SM). We conducted a statistical analysis of tropical cyclones (TCs) generated over the western North Pacific (WNP) between late-April and May. The results showed that there were cases in which TCs were generated before the onset of the SCSSM, accounting for 43.2% of the TCs generated during this season. This study examined a representative case, Super Typhoon Chanchu (0601), which was determined to be influential in the onset of the SCSSM. With a northwestward track, Chanchu brought strong convection and westerly winds to the SCS on 12 May, which triggered the intrusion of the southwesterly winds from the Bay of Bengal and the eastward retreat of the western Pacific subtropical high. Super Typhoon Chanchu provides an example in which a TC triggered the onset of the SCSSM. The negative correlation between the onset date of the SCSSM and the number of TCs generated over the WNP used to be interpreted as the influence of the monsoon trough on TC genesis. This work provides a supplementary illustration that this relationship also includes the impact of TCs on the onset of the SCSSM.
Activation-Induced Conformational Changes of Dopamine D3 Receptor Promote the Formation of the Internal Water Channel
Authors: Weng Wei-Hsiang , Li Ya-Tzu , Hsu Hao-Jen 
Published: October 2017

AbstractThe atomic-level dopamine activation mechanism for transmitting extracellular ligand binding events through transmembrane helices to the cytoplasmic G protein remains unclear. In the present study, the complete dopamine D3 receptor (D3R), with a homology-modeled N-terminus, was constructed to dock different ligands to simulate conformational alterations in the receptor’s active and inactive forms during microsecond-timescale molecular dynamic simulations. In agonist-bound systems, the D3R N-terminus formed a “lid-like” structure and lay flat on the binding site opening, whereas in antagonist and inverse agonist-bound systems, the N-terminus exposed the binding cavity. Receptor activation was characterized using the different molecular switch residue distances, and G protein-binding site volumes. A continuous water pathway was observed only in the dopamine-Gαi-bound system. In the inactive D3Rs, water entry was hindered by the hydrophobic layers. Finally, a complete activation mechanism of D3R was proposed. Upon agonist binding, the “lid-like” conformation of the N-terminus induces a series of molecular switches to increase the volume of the D3R cytoplasmic binding part for G protein association. Meanwhile, water enters the transmembrane region inducing molecular switches to assist in opening the hydrophobic layers to form a continuous water channel, which is crucial for maintaining a fully active conformation for signal transduction.
Association between MGMT Promoter Methylation and Risk of Breast and Gynecologic Cancers: A Systematic Review and Meta-Analysis
Authors: Chen Ru , Zheng Yonglan , Zhuo Lin , Wang Shengfeng 
Published: October 2017

AbstractThe role of the promoter methylation of O6-methylguanine-DNA methyltransferase (MGMT) remains controversial for breast and gynecologic cancers. We conducted a meta-analysis to assess the association between hypermethylation of MGMT promoter and the risk of breast and gynecologic cancers. A comprehensive search was conducted in PubMed and Embase electronic databases up to 19th August 2017 for studies about the association between MGMT promoter hypermethylation and breast and gynecologic cancers. A total of 28 articles including 2,171 tumor tissues and 1,191 controls were involved in the meta-analysis. The pooled results showed that MGMT promoter methylation status was significantly associated with an increased risk of breast and gynecologic cancers (OR = 4.37, 95% CI: 2.68–7.13, P < 0.05). The associations were robust in subgroup analysis based on ethnicity, cancer type, methylation detection method, and control source. This meta-analysis indicated that MGMT hypermethylation was significantly associated with the risk of breast and gynecological cancers, and it may be utilized as a valuable biomarker in early diagnostics and prognostication of these cancers. Further efforts are needed to identify and validate this finding in prospective studies, especially in situation with new methylation testing methods and samples from plasma circulating DNA.
A high-density linkage map and QTL mapping of fruit-related traits in pumpkin (Cucurbita moschata Duch.)
Authors: Zhong Yu-Juan , Zhou Yang-Yang , Li Jun-Xing , Yu Ting , Wu Ting-Quan , Luo Jian-Ning , Luo Shao-Bo , Huang He-Xun 
Published: October 2017

AbstractPumpkin (Cucurbita moschata) is an economically worldwide crop. Few quantitative trait loci (QTLs) were reported previously due to the lack of genomic and genetic resources. In this study, a high-density linkage map of C. moschata was structured by double-digest restriction site-associated DNA sequencing, using 200 F2 individuals of CMO-1 × CMO-97. By filtering 74,899 SNPs, a total of 3,470 high quality SNP markers were assigned to the map spanning a total genetic distance of 3087.03 cM on 20 linkage groups (LGs) with an average genetic distance of 0.89 cM. Based on this map, both pericarp color and strip were fined mapped to a novel single locus on LG8 in the same region of 0.31 cM with phenotypic variance explained (PVE) of 93.6% and 90.2%, respectively. QTL analysis was also performed on carotenoids, sugars, tuberculate fruit, fruit diameter, thickness and chamber width with a total of 12 traits. 29 QTLs distributed in 9 LGs were detected with PVE from 9.6% to 28.6%. It was the first high-density linkage SNP map for C. moschata which was proved to be a valuable tool for gene or QTL mapping. This information will serve as significant basis for map-based gene cloning, draft genome assembling and molecular breeding.
Current Concept in Adult Peripheral Nerve and Brachial Plexus Surgery
Authors: Rasulic Lukas 
Published: October 2017

AbstractPeripheral nerve injuries and brachial plexus injuries are relatively frequent. Significance of these injuries lies in the fact that the majority of patients with these types of injuries constitute working population. Since these injuries may create disability, they present substantial socioeconomic problem nowadays. This article will present current state-of-the-art achievements of minimal invasive brachial plexus and peripheral nerve surgery. It is considered that the age of the patient, the mechanism of the injury, and the associated vascular and soft-tissue injuries are factors that primarily influence the extent of recovery of the injured nerve. The majority of patients are treated using classical open surgical approach. However, new minimally invasive open and endoscopic approaches are being developed in recent years—endoscopic carpal and cubital tunnel release, targeted minimally invasive approaches in brachial plexus surgery, endoscopic single-incision sural nerve harvesting, and there were even attempts to perform endoscopic brachial plexus surgery. The use of the commercially available nerve conduits for bridging short nerve gap has shown promising results. Multidisciplinary approach individually designed for every patient is of the utmost importance for the successful treatment of these injuries. In the future, integration of biology and nanotechnology may fabricate a new generation of nerve conduits that will allow nerve regeneration over longer nerve gaps and start new chapter in peripheral nerve surgery.
Intensification and focusing
Authors: Ghesquière Lobke 
Published: October 2017

Abstract
Intensification or degree modification by means of adverbs and adjectives has been the object of linguistic study for many years, yet there is still a need for clear delineation of this functional category. This study focuses on the differences and similarities between the functional domains of intensification and focusing. The latter function has mainly been described for adverbs, with little attention to its adjectival realizations and to its relation to intensification. By means of corpus studies of pure, purely, mere and merely, it is shown that intensification and focusing are similar to some extent, both engaging in interpersonal modification (McGregor 1997), yet also distinct with intensification being semantically scalar, attitudinal and inherently subjective and focusing being only potentially pragmatically scalar, rhetorical and textually intersubjective.
Noun classification in Kiswahili
Authors: Castagneto Marina 
Published: October 2017

Abstract
The evaluation strategies in Kiswahili display an ambiguous status between inflection (as it was in Proto-Bantu, where classes 12–13, 19 expressed diminution, and classes 20–23 intensification) and derivation. In Modern Kiswahili these classes are lost, and the evaluative category arises by means of a highly productive derivational rule shifting a noun stem to another class: to class 5 for augmentatives, to class 7 for diminutives, because of the semantics of these classes.
The morpheme (−)ji-, originally the prefix marker of class 5, is actually admitted also within a word after another class prefix, sanctioning the birth of noun derivational morphology in Kiswahili: in most cases it has become a morpheme of intensification, but sometimes it can mark a change in size, either augmentative or diminutive.
Intensification and intensifying modification in Hittite
Authors: Dardano Paola 
Published: October 2017

Abstract
The present paper aims to describe intensification and intensifying modification in Hittite, a member of the Anatolian sub-branch of the extensive Indo-European family. Particular attention will be devoted to the following two questions: (i) Which are the most widespread means of intensification in Hittite? and (ii) Which linguistic levels are involved in the expression of intensification (phonological, grammatical, lexical, pragmatic or textual)? Using a corpus-based approach, the study integrates both quantitative and qualitative observations from a theoretical perspective that provides a semantic, syntactic, and pragmatic account of the intensification markers in different documented texts and genres in Hittite.