bluuebunny/crossref_metadata_2025_split huggingface dataset:
A functional variant in TP63 at 3q28 associated with bladder cancer risk by creating an miR‐140‐5p binding site
Authors: Wang Meilin , Du Mulong , Ma Lan , Chu Haiyan , Lv Qiang , Ye Dingwei , Guo Jianming , Gu Chengyuan , Xia Guowei , Zhu Yao , Ding Qiang , Yuan Lin , Fu Guangbo , Tong Na , Qin Chao , Yin Changjun , Xu Jianfeng , Zhang Zhengdong 
Published: December 2015

The first genome‐wide association study (GWAS) for bladder cancer has identified a susceptibility locus at 3q28 in the European ancestry. However, the causal variant at 3q28 remains unknown. We conducted a three‐stage fine mapping study to identify potential causal variants in the region. A total of 41 single nucleotide polymorphisms (SNPs) across 120 kb at 3q28 were tested for association with bladder cancer risk among 3,094 bladder cancer cases and 3,738 controls. Resequencing and functional assays were further evaluated. The SNP rs35592567 in the 3′‐UTR of TP63 was consistently associated with bladder cancer risk in all three stages. In the combined analysis, the T allele of rs35592567 was significantly associated with a decreased risk for bladder cancer (OR = 0.82, 95% CI = 0.75–0.90, P = 9.797 × 10−6 in the additive model). Biochemical assays revealed that the T allele reduced the post‐transcriptional levels of TP63 mediated by interfering binding efficiency of miR‐140‐5p. In addition, overexpression of miR‐140‐5p inhibited bladder cancer cell proliferation and attenuated cell migration, invasion and G1 cell‐cycle arrest. Together, these results suggest that rs35592567 in TP63 may be a novel causal variant contributing to the susceptibility to bladder cancer, which provides additional insight into the pathogenesis of bladder carcinogenesis.
Assessing global and regional iron content in deep gray matter as a function of age using susceptibility mapping
Authors: Liu Manju , Liu Saifeng , Ghassaban Kiarash , Zheng Weili , Dicicco Dane , Miao Yanwei , Habib Charbel , Jazmati Tarek , Haacke E. Mark 
Published: December 2015

PurposeTo investigate the correlation of non‐heme iron content in deep gray matter nuclei as a function of age using quantitative susceptibility mapping (QSM) from both whole‐structural and regional perspectives.Materials and MethodsWe studied a group of 174 normal subjects ranging from 20 to 69 years old and measured the magnetic susceptibility of seven subcortical gray matter nuclei. SWI (susceptibility‐weighted imaging) phase images were used to generate the susceptibility maps, which were acquired on a 1.5T scanner. The 3D whole‐structural measurements were used to determine age‐related thresholds, which were applied to calculate the local iron deposition (RII: portion of the structure that contains iron concentration larger than the structure threshold). Age‐susceptibility correlation was reported for each measured structure for both the whole‐region and two‐region (low iron and high iron content regions) analysis.ResultsFor the local high iron content region, a strong age‐susceptibility correlation was found in the caudate nucleus (CN,R = 0.9), putamen (PUT,R = 0.9), red nucleus (RN,R = 0.8), globus pallidus (GP,R = 0.7), substantia nigra (SN,R = 0.5), and pulvinar thalamus (PT,R = 0.5); for the global iron content, a strong age‐susceptibility correlation was found in CN(R = 0.6), PUT(R = 0.7), and RN(R = 0.6). Overall, for each structure analyzed in this study, regional analysis showed higher correlation coefficient and higher slope comparing to the whole‐region analysis. Further, we found the quantitative conversion factor between magnetic susceptibility and iron concentration to be 1.03 ± 0.03 ppb per μg iron/g wet tissue.ConclusionWe conclude that the age‐susceptibility correlation can serve as a quantitative magnetic susceptibility baseline as a function of age for monitoring abnormal global and regional iron deposition. A regional analysis has shown a tighter age related behavior, providing a reliable and sensitive reference for what can be considered normal iron content for studies of neurodegenerative diseases. J. Magn. Reson. Imaging 2016;44:59–71.
The atmospheric circulation characteristics favouring snowfall in an area with complex relief in Northwestern Greece
Authors: Dafis S. , Lolis C. J. , Houssos E. E. , Bartzokas A. 
Published: December 2015

ABSTRACTThe connection between atmospheric circulation over Europe and snowfall in Ioannina, Northwestern (NW) Greece, is examined. The study deals with 160 snow events, referring to the 56‐year period 1956–2011. For each of the events, the patterns of 500 and 1000 hPa geopotential height, 500 and 850 hPa air temperature, 1000–500, 1000–700 and 700–500 hPa thickness are constructed for the European area, whereas static stability K‐index, relative humidity at 500, 700 and 850 hPa levels and finally relative vorticity at 500 and 1000 hPa are also used for the Southeastern (SE) Mediterranean region, for the previous day (D‐1), the starting day (D) and the day following the cessation of snowfall (END). Factor analysis and cluster analysis are applied to the above datasets and the evolutions of the above circulation parameters are classified into eight clusters. Snowfall in NW Greece is generally associated with a low pressure system over the Ionian Sea combined with an anticyclone over Western or NW Europe, causing cold northwesterly flow in the lower troposphere over NW Greece. In the middle troposphere, a deep 500 hPa trough usually extends from Western Russia to Italy and the Ionian Sea. The eight atmospheric circulation structures differ mainly in the exact location and/or the intensity of the depression and/or the anticyclone, in the orientation of the 500 hPa trough axis and in the trajectories of the above synoptic systems between D‐1 and END days.
Better knowledge with more gauges? Investigation of the spatiotemporal characteristics of precipitation variations over the Greater Beijing Region
Authors: Yang Wen‐Yu , Li Zhe , Sun Ting , Ni Guang‐Heng 
Published: December 2015

ABSTRACTUsing the hourly precipitation observations from 118 gauge stations and a weather radar in the Greater Beijing Region (GBR) during 2008–2012, we investigate the spatiotemporal characteristics of precipitation and discuss the appropriate observational approach for capturing variability of precipitation over this region. In general, the south central and northeastern GBR receives more intense precipitation than other parts. The diurnal cycle of precipitation amount (PA) peaks in the evening and decreases till noon, whereas precipitation intensity (PI) and precipitation frequency (PF) both have two peaks. The stronger peaks of PI and PF occur in the evening whereas the weaker ones appear in the early nighttime and in the afternoon. Remarkable spatial heterogeneity also exists in the diurnal patterns of PA, PI and PF over the GBR. Rainstorms extracted from radar data feature in short duration (11.4 h in average) and highly localized patterns (4.31–20.58/1.85–9.10 km in major/minor radius direction). The estimated diurnal cycles of PA, PI and PF are found to depend on the gauge density in a sensitivity analysis, where a gauge density ratio of 0.6 (corresponding to 30 gauges in total with a representative area of 239.5 km2 per gauge) is identified as adequate to capture the temporal characteristics of precipitation in the plain area of GBR. However, such a gauge density ratio (i.e. 0.6) is incapable for resolving the spatial characteristics of precipitation in GBR. As such, different instruments (e.g. gauge network, weather radar, etc.) and multiple data sources are suggested to be jointly utilized to better capture the characteristics of rainstorms in GBR.
Development of high‐resolution (250 m) historical daily gridded air temperature data using reanalysis and distributed sensor networks for the US Northern Rocky Mountains
Authors: Holden Zachary A. , Swanson Alan , Klene Anna E. , Abatzoglou John T. , Dobrowski Solomon Z. , Cushman Samuel A. , Squires John , Moisen Gretchen G. , Oyler Jared W. 
Published: December 2015

ABSTRACTGridded temperature data sets are typically produced at spatial resolutions that cannot fully resolve fine‐scale variation in surface air temperature in regions of complex topography. These data limitations have become increasingly important as scientists and managers attempt to understand and plan for potential climate change impacts. Here, we describe the development of a high‐resolution (250 m) daily historical (1979–2012) temperature data set for the US Northern Rocky Mountains using observations from both long‐term weather stations and a dense network of low‐cost temperature sensors. Empirically based models for daily minimum and maximum temperature incorporate lapse rates from regional reanalysis data, modelled daily solar insolation and soil moisture, along with time invariant canopy cover and topographic factors. Daily model predictions demonstrate excellent agreement with independent observations, with mean absolute errors of <1.4 °C for both minimum and maximum temperature. Topographically resolved temperature data may prove useful in a range of applications related to hydrology, fire regimes and fire behaviour, and habitat suitability modelling. The form of the models may provide a means for downscaling future temperature scenarios that account for potential fine‐scale topographically mediated changes in near‐surface temperature.
Rheology and microstructure of binary mixed gel of rice bran protein–whey: effect of heating rate and whey addition
Authors: Rafe Ali , Vahedi Elnaz , Hasan‐Sarei Azadeh Ghorbani 
Published: December 2015

AbstractBACKGROUNDRice bran protein (RBP) is a valuable plant protein which has unique nutritional and hypoallergenic properties. Whey proteins have wide applications in the food industry, such as in dairy, meat and bakery products.RESULTSWhey protein concentrate (WPC), RBP and their mixtures at different ratios (1:1, 1:2, 1:5 and 1:10 w/w) were heated from 20 to 90 °C at different heating rates (0.5, 1, 5 and 10 °C min−1). The storage modulus (G′) and gelling point (Tgel) of WPC were higher than those of RBP, indicating the good ability of WPC to develop stiffer networks. By increasing the proportion of WPC in mixed systems, G′ was increased and Tgel was reduced. Nevertheless, the elasticity of all binary mixtures was lower than that of WPC alone. Tgel and the final G′ of RBP–WPC blends were increased by raising the heating rate. The RBP–WPC mixtures developed more elastic gels than RBP alone at different heating rates. RBP had a fibrillar and lentil‐like structure whose fibril assembly had smaller structures than those of WPC.CONCLUSIONThe gelling structure of the mixed gel of WPC–RBP was improved by adding WPC. Indeed, by adding WPC, gels tended to show syneresis and had lower water‐holding capacity. Furthermore, the gel structure was produced by adding WPC to the non‐gelling RBP, which is compatible with whey and can be applied as a functional food for infants and/or adults. © 2015 Society of Chemical Industry
Selection of lactic acid bacteria strains for the hydrolysis of allergenic proteins of wheat flour
Authors: Stefańska Ilona , Piasecka‐Jóźwiak Katarzyna , Kotyrba Danuta , Kolenda Magdalena , Stecka Krystyna M 
Published: December 2015

AbstractBackgroundWheat flour is one of the most common causative agents of food allergy. The study presents the selection and characterization of lactic acid bacteria (LAB) strains capable of hydrolyzing/modifying allergenic proteins of wheat flour. Hydrolysis of wheat proteins was determined with sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting with sera from patients with food allergy to gluten.ResultsThe analysis of electrophoretic profiles of protein extracted from sourdough shows the capability of selected LAB strains for proteolytic degradation of wheat proteins that belong to two factions: albumin/globulin (hydrolysis of 13 polypeptides with a molecular weight between 103 and 22 kDa); and gliadin (seven polypeptides with a molecular weight between 39 and 24 kDa). All analyzed strains were capable of hydrolyzing some IgE‐binding epitopes of wheat allergens. The lack of such changes in control samples indicates that they were induced rather by the proteolytic activity of bacterial strains than endogenous enzymes of wheat flour. The gluten proteins were susceptible to hydrolysis by sequential digestion with pepsin and trypsin.ConclusionThe selected strains exhibit proteolytic activity, which leads to a reduction in allergenicity of wheat sourdoughs. These strains may be applied as specific starter cultures to prepare bakery products of special nutritional use. © 2015 Society of Chemical Industry
Short‐ and long‐term effects of carbohydrate limitation on sugar and organic acid accumulation during mandarin fruit growth
Authors: Antoine Sandrine , Pailly Olivier , Gibon Yves , Luro François , Santini Jérémie , Giannettini Jean , Berti Liliane 
Published: December 2015

AbstractBACKGROUNDThe physiological roles of organic acids in fruit cells are not fully understood, especially in citrus, whereas the decline in titratable acidity during ripening shown by many citrus fruits is due to the utilization of citric acid. We induced carbohydrate depletion by removing source leaves at two key periods in mandarin development (early and full citric acid accumulation). Then, we assessed the resulting changes in the short term (within 48 h) and long term (several weeks until ripening).RESULTSControl mature fruits were characterized by elevated fresh weight, large diameters and high quantities of malic acid, citric acid and sucrose. At the same stage, fruits subjected to early or late defoliation had higher glucose, fructose, citric acid concentrations and lower sucrose concentrations. They differed only in their malic acid concentrations, which were higher in early defoliation fruits and similar in late defoliation fruits when compared to control fruits. Finally, fruits subjected to late defoliation were characterized by high proline and γ‐aminobutyric acid concentrations, and low fructose and glucose concentrations.CONCLUSIONWe have shown that short‐ and long‐term carbohydrate limitation modifies sugar and organic acid metabolism during mandarin fruit growth. © 2015 Society of Chemical Industry
Influence of substituent position and cavity size of the regioisomers of monocarboxymethyl‐α‐, β‐, and γ‐cyclodextrins on the apparent stability constants of their complexes with both enantiomers of Tröger's base
Authors: Řezanka Pavel , Řezanková Klára , Sedláčková Helena , Mašek Jindřich , Rokosová Lenka , Bláhová Markéta , Řezanka Michal , Jindřich Jindřich , Sýkora David , Král Vladimír 
Published: December 2015

Enantiomers of Tröger's base were separated by capillary electrophoresis using 2I‐O‐, 3I‐O‐, and 6I‐O‐carboxymethyl‐α‐, β‐, and γ‐cyclodextrin and native α‐, β‐, and γ‐cyclodextrin as chiral additives at 0–12 mmol/L for β‐cyclodextrin and its derivatives and 0–50 mmol/L for α‐ and γ‐cyclodextrins and their derivatives in a background electrolyte composed of sodium phosphate buffer at 20 mmol/L concentration and pH 2.5. Apparent stability constants of all cyclodextrin–Tröger's base complexes were calculated based on capillary electrophoresis data. The obtained results showed that the position of the carboxymethyl group as well as the cavity size of the individual cyclodextrin significantly influences the apparent stability constants of cyclodextrin–Tröger's base complexes.
Surface plasmon polariton assisted optical pulling force
Authors: Petrov Mihail I. , Sukhov Sergey V. , Bogdanov Andrey A. , Shalin Alexander S. , Dogariu Aristide 
Published: December 2015

AbstractWe demonstrate both analytically and numerically the existence of optical pulling forces acting on particles located near plasmonic interfaces. Two main factors contribute to the appearance of this negative recoil force. The interference between the incident and reflected waves induces a rotating dipole with an asymmetric scattering pattern, while the directional excitation of surface plasmon polaritons (SPPs) enhances the linear momentum of scattered light. The strongly asymmetric SPP excitation is determined by spin–orbit coupling of the rotating dipole and surface plasmon polariton. As a result of the total momentum conservation, the force acting on the particle points in a direction opposite to the incident wave propagation. We derive analytical expressions for the force acting on dipolar particles placed in the proximity of plasmonic surfaces. Analytical expressions for this pulling force are derived within the dipole approximation and are in excellent agreement with results of electromagnetic numerical calculations. The forces acting on larger particles are analyzed numerically, beyond the dipole approximation.
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Integrated safety of levodopa‐carbidopa intestinal gel from prospective clinical trials
Authors: Lang Anthony E. , Rodriguez Ramon L. , Boyd James T. , Chouinard Sylvain , Zadikoff Cindy , Espay Alberto J. , Slevin John T. , Fernandez Hubert H. , Lew Mark F. , Stein David A. , Odin Per , Fung Victor S.C. , Klostermann Fabian , Fasano Alfonso , Draganov Peter V. , Schmulewitz Nathan , Robieson Weining Z. , Eaton Susan , Chatamra Krai , Benesh Janet A. , Dubow Jordan 
Published: December 2015

ABSTRACTBackgroundContinuous administration of levodopa‐carbidopa intestinal gel (carbidopa‐levodopa enteral suspension) through a percutaneous endoscopic gastrojejunostomy is a treatment option for advanced Parkinson disease (PD) patients with motor fluctuations resistant to standard oral medications. Safety data from 4 prospective studies were integrated to assess the safety of this therapy.MethodsSafety data from 4 studies were summarized using 2 overlapping data sets, permitting the separation of procedure/device–associated (n = 395) from non‐procedure/device adverse events (n = 412).ResultsAt the data cutoff, median exposure to levodopa‐carbidopa intestinal gel was 911 days (range, 1‐1980 days) with 963 total patient‐years of exposure. Procedure/device adverse events occurred in 300 patients (76%), and serious adverse events occurred in 68 (17%); most frequently reported procedure/device adverse events and serious adverse events were complications of device insertion (41% and 8%, respectively) and abdominal pain (36% and 4%, respectively). Non‐procedure/device adverse events occurred in 92% (379), with most frequently reported being insomnia (23%) and falls (23%); 42% (171) had non‐procedure/device serious adverse events, with most frequently reported being pneumonia (5%) and PD symptoms (2%). Adverse events led to discontinuation in 17% (72), most frequently because of complication of device insertion (2.4%). There were 34 treatment‐emergent deaths (8.3%) in the overlapping data sets, 2 of which (0.5%) were considered “possibly related” to the treatment system.ConclusionIn the largest collection of levodopa‐carbidopa intestinal gel safety data from prospective clinical studies, procedure/device events were frequently reported and occasionally life threatening. Most non‐procedure/device events were typical for levodopa treatment and an elderly population. These factors combined with high treatment efficacy led to a relatively low discontinuation rate in advanced PD patients. © 2015 International Parkinson and Movement Disorder Society
Myosteatosis increases with aging and is associated with incident diabetes in African ancestry men
Authors: Miljkovic Iva , Kuipers Allison L. , Cvejkus Ryan , Bunker Clareann H. , Patrick Alan L. , Gordon Christopher L. , Zmuda Joseph M. 
Published: December 2015

ObjectiveSkeletal muscle fat infiltration (known as myosteatosis) is greater in African compared with European ancestry men and may play an important role in the development of type 2 diabetes (T2D). However, prospective studies examining the magnitude of changes in myosteatosis with aging and their metabolic consequences are sparse.MethodsLongitudinal changes in peripheral quantitative computed tomography measured calf myosteatosis [intermuscular fat (mm2) and skeletal muscle density as a measure of intramuscular fat (mg/cm3)] were examined in 1515 Afro‐Caribbean men aged 40+ years recruited without regard to their health status.ResultsDuring an average of 6.2 years of follow‐up, an age‐related increase in intermuscular fat and a decrease in skeletal muscle density were observed (all P < 0.0001), which remained significant in those who lost weight, gained weight, or remained weight stable (all P < 0.0001). In addition, muscle density loss accelerated with increasing age (P < 0.0001). Increased intermuscular fat during follow‐up was associated with an increased incident risk of T2D independent of factors known to be associated with T2D (odds ratios per 1‐SD increase in intermuscular fat = 1.29; 95% CI = 1.08‐1.53).ConclusionsOur findings suggest that both inter‐ and intramuscular fat increase with advancing age and that intermuscular fat contributes to development of T2D among African ancestry men.
Generic switching of warfarin and risk of excessive anticoagulation: a Danish nationwide cohort study
Authors: Hellfritzsch Maja , Rathe Jette , Stage Tore Bjerregaard , Thirstrup Steffen , Grove Erik L. , Damkier Per , Pottegård Anton 
Published: December 2015

AbstractPurposeGeneric switching of warfarin was recently repealed in Denmark, as adverse drug reaction (ADR) reports suggested risk of excessive anticoagulation following switches from branded to generic warfarin. We investigated this putative association in a formalized pharmacoepidemiological analysis.MethodsWe conducted a nationwide cohort study based on Danish healthcare registries, including data from the introduction of generic warfarin until the repeal (January 2011–April 2015). We followed Danish warfarin users over time and compared the rate of incident hospitalizations due to excessive anticoagulation (i.e. increased INR or any bleeding requiring hospitalization) in periods following a recent switch to generic warfarin to the rate in periods without a recent switch.ResultsWe included 105 751 warfarin users, filling a total of 1 539 640 prescriptions for warfarin (2.5% for generic warfarin). This constituted 89.0% of all warfarin prescriptions in Denmark during the study period. We observed 19 362 switches to generic warfarin during the study period. The adjusted hazard ratio for excessive anticoagulation following a recent switch from branded to generic warfarin was 1.1 (95%CI, 0.8–1.4). The result was robust within subgroups and several sensitivity analyses.ConclusionSwitching from branded to generic warfarin is not associated with an increased risk of hospitalization with excessive anticoagulation. However, a minor excess risk of transient INR increase cannot be excluded. Pharmacoepidemiological studies provide an effective method for swift evaluation of hypotheses generated by ADR‐reports. Copyright © 2015 John Wiley & Sons, Ltd.