bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing dermoscopy and histological examination of normal equine skin
Authors: Tomich Lara M. , Pieper Jason B. , Stern Adam W. 
Published: November 2017

BackgroundDermoscopy is a noninvasive diagnostic technique that allows visualization of structures of the superficial dermis not visible with the naked eye.Hypothesis/ObjectivesTo assess the usefulness and applicability of dermoscopy for evaluation of healthy equine skin.AnimalsTwelve healthy horses from a research herd.MethodsFive regions (cheek, lateral neck, dorsum, flank and abdomen) were examined with contact dermoscopy using both nonpolarized and polarized light at both 17‐fold and 24‐fold magnification. These findings were compared to histological features of skin biopsies cut both longitudinally and transversely.ResultsUsing a hand‐held dermatoscope with nonpolarized light, epidermal ridges were observed. Using polarized light, follicular openings and distinctly separate epidermal openings of sweat gland ducts were observed in some but not all individuals. Similarities were noted between histological and dermoscopic results.ConclusionsAlthough not ideal for visualizing many structures in the superficial dermis of healthy equine skin, dermoscopy allowed visualization of epidermal ridges, hair shafts in the infundibular portion of the hair follicles and sweat gland duct openings. Dermoscopy could potentially be useful in the evaluation of diseases affecting the sweat glands, epidermis and hair shaft.
Evaluating the potential of Unmanned Aerial Systems for mapping weeds at field scales: a case study with Alopecurus myosuroides
Authors: Lambert J P T , Hicks H L , Childs D Z , Freckleton R P 
Published: November 2017

SummaryMapping weed densities within crops has conventionally been achieved either by detailed ecological monitoring or by field walking, both of which are time‐consuming and expensive. Recent advances have resulted in increased interest in using Unmanned Aerial Systems (UAS) to map fields, aiming to reduce labour costs and increase the spatial extent of coverage. However, adoption of this technology ideally requires that mapping can be undertaken automatically and without the need for extensive ground‐truthing. This approach has not been validated at large scale using UAS‐derived imagery in combination with extensive ground‐truth data. We tested the capability of UAS for mapping a grass weed, Alopecurus myosuroides, in wheat crops. We addressed two questions: (i) can imagery accurately measure densities of weeds within fields and (ii) can aerial imagery of a field be used to estimate the densities of weeds based on statistical models developed in other locations? We recorded aerial imagery from 26 fields using a UAS. Images were generated using both RGB and Rmod (Rmod 670–750 nm) spectral bands. Ground‐truth data on weed densities were collected simultaneously with the aerial imagery. We combined these data to produce statistical models that (i) correlated ground‐truth weed densities with image intensity and (ii) forecast weed densities in other fields. We show that weed densities correlated with image intensity, particularly Rmod image data. However, results were mixed in terms of out of sample prediction from field‐to‐field. We highlight the difficulties with transferring models and we discuss the challenges for automated weed mapping using UAS technology.
Visual field indices and patterns of visual field deficits in mesopic and dark-adapted two-colour fundus-controlled perimetry in macular diseases
Authors: Pfau Maximilian , Lindner Moritz , Steinberg Julia S , Thiele Sarah , Brinkmann Christian K , Fleckenstein Monika , Holz Frank G , Schmitz-Valckenberg Steffen 
Published: November 2017

Background/AimsTo analyse the retest reliability of visual field indices and to describe patterns of visual field deficits in mesopic and dark-adapted two-colour fundus-controlled perimetry (FCP) in macular diseases.MethodsSeventy-seven eyes (30 eyes with macular diseases and 47 normal eyes) underwent duplicate mesopic and dark-adapted two-colour FCP (Scotopic Macular Integrity Assessment, CenterVue). Non-weighted (mean defect, loss variance), variability-weighted (mean deviation, pattern standard deviation (PSD)) and graphical (cumulative defect (Bebie) curves) indices were computed. Reproducibility (coefficient of repeatability, CoR) of these indices was assessed. Cluster analysis was carried out to identify patterns of visual field deficits.ResultsThe intrasession reproducibility was lower for the mean defect as compared with the mean deviation (CoR (dB) 2.67 vs 2.57 for mesopic, 1.71 vs 1.45 for dark-adapted cyan, 1.94 vs 1.87 for dark-adapted red testing) and lower for the square-root loss variance as compared with the PSD (CoR (dB) 1.48 vs 1.34, 0.77 vs 0.65, 1.23 vs 1.03). Hierarchical cluster analysis of the indices disclosed six patterns of visual field deficits (approximately unbiased P value>0.95) with varying degrees of global versus focal defect and rod versus cone dysfunction. These were also reflected by the cumulative defect curves.ConclusionFCP with mesopic and dark-adapted two-colour testing allows for reproducible assessment of different types of retinal sensitivity, whereby mean deviation and PSD exhibited the better retest reliability of the tested indices. Distinct patterns of retinal dysfunction can be identified using this setup, reflecting variable degrees of rod and cone dysfunction in different macular diseases. Dark-adapted two-colour FCP provides additional diagnostic information and allows for refined structure–function correlation in macular diseases.
Menopausal hormone therapy and colorectal cancer: a linkage between nationwide registries in Norway
Authors: Botteri Edoardo , Støer Nathalie C , Sakshaug Solveig , Graff-Iversen Sidsel , Vangen Siri , Hofvind Solveig , de Lange Thomas , Bagnardi Vincenzo , Ursin Giske , Weiderpass Elisabete 
Published: November 2017

ObjectivesWith the present study, we aimed to investigate the association between menopausal hormone therapy (HT) and risk of colorectal cancer (CRC).SettingCohort study based on the linkage of Norwegian population-based registries.ParticipantsWe selected 466822 Norwegian women, aged 55–79, alive and residing in Norway as of 1 January 2004, and we followed them from 2004 to 2008. Each woman contributed person-years at risk as non-user, current user and/or past HT user.Outcome measuresThe outcome of interest was adenocarcinoma of the colorectal tract, overall, by anatomic site and stage at diagnosis. Incidence rate ratios (RRs) with 95% CIs were estimated by Poisson regression and were used to evaluate the association between HT and CRC incidence.ResultsDuring the median follow-up of 4.8 years, 138 655 (30%) women received HT and 3799 (0.8%) incident CRCs occurred. Current, but not past, use of HT was associated with a lower risk of CRC (RR 0.88; 95% CI 0.80 to 0.98). RRs for localised, regionally advanced and metastatic CRC were 1.13 (95% CI 0.91 to 1.41), 0.81 (95% CI 0.70 to 0.94) and 0.79 (95% CI 0.62 to 1.00), respectively. RRs for current use of oestrogen therapy (ET) were 0.91 (95% CI 0.80 to 1.04) while RR for current use of combined oestrogen–progestin therapy (EPT) was 0.85 (95% CI 0.70 to 1.03), as compared with no use of HT. The same figures for ET and EPT in oral formulations were 0.83 (95% CI 0.68 to 1.03) and 0.86 (95% CI 0.71 to 1.05), respectively.ConclusionsIn our nationwide cohort study, HT use lowered the risk of CRC, specifically the most advanced CRC.
Cross-sectional study of the financial cost of training to the surgical trainee in the UK and Ireland
Authors: O’Callaghan John , Mohan Helen M , Sharrock Anna , Gokani Vimal , Fitzgerald J Edward , Williams Adam P , Harries Rhiannon L 
Published: November 2017

ObjectivesApplications for surgical training have declined over the last decade, and anecdotally the costs of training at the expense of the surgical trainee are rising. We aimed to quantify the costs surgical trainees are expected to cover for postgraduate training.DesignProspective, cross-sectional, questionnaire-based study.Setting/ParticipantsA non-mandatory online questionnaire for UK-based trainees was distributed nationally. A similar national questionnaire was distributed for Ireland, taking into account differences between the healthcare systems. Only fully completed responses were included.ResultsThere were 848 and 58 fully completed responses from doctors based in the UK and Ireland, respectively. Medical students in the UK reported a significant increase in debt on graduation by 55% from £17 892 (2000–2004) to £27 655 (2010–2014) (p<0.01). 41% of specialty trainees in the UK indicated that some or all of their study budget was used to fund mandatory regional teaching. By the end of training, a surgical trainee in the UK spends on average £9105 on courses, £5411 on conferences and £4185 on exams, not covered by training budget. Irish trainees report similarly high costs. Most trainees undertake a higher degree during their postgraduate training. The cost of achieving the mandatory requirements for completion of training ranges between £20 000 and £26 000 (dependent on specialty), except oral and maxillofacial surgery, which is considerably higher (£71 431).ConclusionsMedical students are graduating with significantly larger debt than before. Surgical trainees achieve their educational requirements at substantial personal expenditure. To encourage graduates to pursue and remain in surgical training, urgent action is required to fund the mandatory requirements and annual training costs for completion of training and provide greater transparency to inform doctors of what their postgraduate training costs will be. This is necessary to increase diversity in surgery, reduce debt load and ensure surgery remains a popular career choice.
Exercise induces muscle fiber type switching via transient receptor potential melastatin 2-dependent Ca2+ signaling
Authors: Lee Seo-Ho , Kim Byung-Ju , Park Dae-Ryoung , Kim Uh-Hyun 
Published: November 2017

The aim of the present study was to examine whether transient receptor potential melastatin 2 (TRPM2) plays a role in muscle fiber-type transition during exercise. Mice were trained at a speed of 12 m/min at a slope of 0° for 60 min for 5 consecutive days/wk for 4 wk. Exhaustion tests were performed on the treadmill (the speed was set at 6 m/min at a slope of 0° and increased at a rate of 1 m/min every 6 min). Isolated primary skeletal muscle cells from TRPM2-knockout (KO) mice showed lower amplitudes of electrical stimuli (ES)-induced Ca2+ signals when compared with wild-type (WT) mice due to a defect in Ca2+ influx. Moreover, TRPM2-KO mice had a higher proportion of fast-twitch skeletal muscle fibers and a lower proportion of slow-twitch muscle fibers before exercise than WT mice. After exercise, the expression of slow-twitch skeletal muscle fibers was increased only in WT mice but not in TRPM2-KO mice. ES-induced nuclear translocation of the Ca2+-dependent transcription factor NFATc1 was significantly lower in TRPM2-KO mice than in WT mice. TRPM2-KO mice also showed decreased mitochondrial Ca2+ and membrane potential. Lactate levels were higher in the skeletal muscle cells of TRPM2-KO mice before and after ES compared with WT mice. Collectively, these data indicate that TRPM2-mediated Ca2+ signaling plays a critical role in the regulation of fiber-type switching and mitochondrial function in skeletal muscle. NEW & NOTEWORTHY TRPM2 has been shown to play an important role in a variety of cellular functions. However, the role of TRPM2 in skeletal muscle remains poorly understood. Here, we provide evidence that TRPM2-mediated Ca2+ signaling is required for training-induced improvement in skeletal muscle mitochondrial function and fiber type transition.
Cycling efficiency and energy cost of walking in young and older adults
Authors: Gaesser Glenn A. , Tucker Wesley J. , Sawyer Brandon J. , Bhammar Dharini M. , Angadi Siddhartha S. 
Published: November 2017

To determine whether age affects cycling efficiency and the energy cost of walking (Cw), 190 healthy adults, ages 18–81 yr, cycled on an ergometer at 50 W and walked on a treadmill at 1.34 m/s. Ventilation and gas exchange at rest and during exercise were used to calculate net Cw and net efficiency of cycling. Compared with the 18–40 yr age group (2.17 ± 0.33 J·kg−1·m−1), net Cw was not different in the 60–64 yr (2.20 ± 0.40 J·kg−1·m−1) and 65–69 yr (2.20 ± 0.28 J·kg−1·m−1) age groups, but was significantly ( P < 0.03) higher in the ≥70 yr (2.37 ± 0.33 J·kg−1·m−1) age group. For subjects >60 yr, net Cw was significantly correlated with age ( R2 = 0.123; P = 0.002). Cycling net efficiency was not different between 18–40 yr (23.5 ± 2.9%), 60–64 yr (24.5 ± 3.6%), 65–69 yr (23.3 ± 3.6%) and ≥70 yr (24.7 ± 2.7%) age groups. Repeat tests on a subset of subjects (walking, n = 43; cycling, n = 37) demonstrated high test-retest reliability [intraclass correlation coefficients (ICC), 0.74–0.86] for all energy outcome measures except cycling net energy expenditure (ICC = 0.54) and net efficiency (ICC = 0.50). Coefficients of variation for all variables ranged from 3.1 to 7.7%. Considerable individual variation in Cw and efficiency was evident, with a ~2-fold difference between the least and most economical/efficient subjects. We conclude that, between 18 and 81 yr, net Cw was only higher for ages ≥70 yr, and that cycling net efficiency was not different across age groups.NEW & NOTEWORTHY This study illustrates that the higher energy cost of walking in older adults is only evident for ages ≥70 yr. For older adults ages 60–69 yr, the energy cost of walking is similar to that of young adults. Cycling efficiency, by contrast, is not different across age groups. Considerable individual variation (∼2-fold) in cycling efficiency and energy cost of walking is observed in young and older adults.
Does Anaemia Have Major Public Health Importance in Children Aged 6–59 Months in the Duggina Fanigo District of Wolaita Zone, Southern Ethiopia?
Authors: Tiku Yohannes Samuel , Mekonnen Tefera Chane , Workie Shimelash Bitew , Amare Endale 
Published: November 2017

<b><i>Background:</i></b> Globally, a large number of children aged 6–59 months are affected by anaemia. In Ethiopia, like other developing countries, more than 40% of children under 5 years are affected by anaemia. Anaemia during infancy and childhood period is associated with poor health and impaired cognitive development, leading to reduced academic achievement and earning potential in their adult life. The aim of this research was to assess whether anaemia remained a major public health problem among children aged 6–59 months or not in Duggina Fanigo District of Wolaita Zone, South Ethiopia in 2016. <b><i>Methodology:</i></b> A community-based cross-sectional study was conducted from February to March 2016, on 404 mothers with children aged 6–59 months who were selected through the systematic sampling method. Socio-demographic and other data on associated factors was collected using a pre-tested questionnaire. Capillary blood was taken from the fingertip of each child and hemoglobin was measured using Haemo-Cue digital photometer. All the necessary safety measures were taken during blood collection. Data analysis was made using SPSS version 21. Multivariable logistic regression analysis was used to assess the association of independent variables with outcome variables and to control the possible confounding factors. <b><i>Result:</i></b> The overall prevalence of anaemia was 51.4%. Anaemia was common among young children as compared to older children. After controlling the effect of confounding and adjusting for age, gender and altitude, explanatory variables like low dietary diversity (AOR = 3.24; 95% CI [1.68–6.23]), food insecurity (AOR = 3.63; 95% CI [2.18–6.04]), stunting (AOR = 2.60; 95% CI [1.56–4.35]), underweight (AOR = 2.46; 95% CI [1.29–4.67]) and fever within 2 weeks (AOR = 2.49; 95% CI [1.29–4.81]) prior to the survey were significantly associated with anaemia. In conclusion, the overall prevalence of anaemia among children aged 6–59 months has remained a major public health importance in the study area. Integrated efforts need to be prioritized to improve the health as well as the nutritional status of children aged 6–59 months through appropriate feeding practices in infants and children.
Multicenter Evaluation of Octreotide as Secondary Prophylaxis in Patients With Left Ventricular Assist Devices and Gastrointestinal Bleeding
Authors: Shah Keyur B. , Gunda Sampath , Emani Sitaramesh , Kanwar Manreet K. , Uriel Nir , Colombo Paolo C. , Uber Patricia A. , Sears Melissa L. , Chuang Joyce , Farrar David J. , Brophy Donald F. , Smallfield George B. 
Published: November 2017

Background
Gastrointestinal (GI) bleeding is one of the most common complications after continuous-flow left ventricular assist device implantation. More than one third of patients with incident bleed go on to develop recurrent GI bleeding. Octreotide, a somatostatin analog, is proposed to reduce the risk of recurrent GI bleeding in this population.

Methods and Results

This multicenter, retrospective analysis evaluated 51 continuous-flow left ventricular assist device patients who received secondary prophylaxis with octreotide after their index GI bleed from 2009 to 2015. All patients had a hospitalization for GI bleed and received octreotide after discharge. Patient demographics, medical and medication history, and clinical characteristics of patients who rebled after receiving octreotide were compared with non–rebleeders. These data were also compared with matched historical control patients previously enrolled in the HMII (HeartMate II) clinical trials, none of whom received octreotide, to provide a context for the bleeding rates. Twelve patients (24%) who received secondary octreotide prophylaxis developed another GI bleed, whereas 39 (76%) did not. There were similar intergroup demographics; however, significantly more bleeders had a previous GI bleeding history before left ventricular assist device placement (33% versus 5%;
P
=0.02) and greater frequency of angiodysplasia confirmed during endoscopy (58% versus 23%;
P
=0.03). Fewer patients in this study experienced a recurrent GI bleed compared with a matched historical control group that did not receive octreotide (24% versus 43%;
P
=0.04).

Conclusions
Patients with continuous-flow left ventricular assist device receiving secondary prophylaxis with octreotide had a significantly lower GI bleed recurrence compared with historical controls not treated with octreotide. Additional prospective studies are needed to confirm these data.
2-Cl-MGV-1 Ameliorates Apoptosis in the Thalamus and Hippocampus and Cognitive Deficits After Cortical Infarct in Rats
Authors: Chen Yicong , Veenman Leo , Singh Sukhdev , Ouyang Fubing , Liang Jiahui , Huang Weixian , Marek Ilan , Zeng Jinsheng , Gavish Moshe 
Published: November 2017

Background and Purpose—
Focal cortical infarction causes neuronal apoptosis in the ipsilateral nonischemic thalamus and hippocampus, which is potentially associated with poststroke cognitive deficits. TSPO (translocator protein) is critical in regulating mitochondrial apoptosis pathways. We examined the effects of the novel TSPO ligand 2-(2-chlorophenyl) quinazolin-4-yl dimethylcarbamate (2-Cl-MGV-1) on poststroke cognitive deficits, neuronal mitochondrial apoptosis, and secondary damage in the ipsilateral thalamus and hippocampus after cortical infarction.

Methods—
One hundred fourteen hypertensive rats underwent successful distal middle cerebral artery occlusion (n=76) or sham procedures (n=38). 2-Cl-MGV-1 or dimethyl sulfoxide as vehicle was administrated 2 hours after distal middle cerebral artery occlusion and then for 6 or 13 days (n=19 per group). Spatial learning and memory were tested using the Morris water maze. Secondary degeneration and mitochondrial apoptosis in the thalamus and hippocampus were assessed using Nissl staining, immunohistochemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling, JC-1 staining, and immunoblotting 7 and 14 days after surgery.

Results—

Infarct volumes did not significantly differ between the vehicle and 2-Cl-MGV-1 groups. There were more neurons and fewer glia in the ipsilateral thalamus and hippocampus in the vehicle groups than in the sham-operated group 7 and 14 days post–distal middle cerebral artery occlusion. 2-Cl-MGV-1 significantly ameliorated spatial cognitive impairment and decreased neuronal death and glial activation when compared with vehicle treatment (
P
<0.05). The collapse of mitochondrial transmembrane potential and cytoplasmic release of apoptosis-inducing factors and cytochrome
c
was prevented within the thalamus. Caspase cleavage and the numbers of terminal deoxynucleotidyl transferase dUTP nick end labeling
+
or Nissl atrophic cells were reduced within the thalamus and hippocampus. This was accompanied by upregulation of B-cell lymphoma 2 and downregulation of Bax (
P
<0.05).

Conclusions—
2-Cl-MGV-1 reduces neuronal apoptosis via mitochondrial-dependent pathways and attenuates secondary damage in the nonischemic thalamus and hippocampus, potentially contributing to ameliorated cognitive deficits after cortical infarction.
A new species of Thalerosphyrus Eaton 1881 (Ephemeroptera: Heptageniidae: Ecdyonurinae) from India
Authors: SELVAKUMAR C. , CHANDRA KAILASH , SIVARAMAKRISHNAN K. G. , JEHAMALAR E. EYARIN 
Published: November 2017

A new species of Thalerosphyrus Eaton 1881 is described based on larvae collected from East Khasi Hills district and East Jaintia Hills district of Meghalaya. Thalerosphyrus meghalayensis Selvakumar & Chandra n. sp. can be distinguished from other known species of the genus by the following combination of characters: (i) posterolateral expansions of segment VIII always shorter than those of segment VII; (ii) pronotum moderately enlarged laterally; (iii) bristles on the dorsal face of hind femora truncate or round at apex; (iv) hind tibia with a row of thin setae; (v) outer margin of superlinguae evenly covered with long setae ending at apex by minute setae and (vi) posterior margin of tergites with regularly pointed teeth, and numerous microdenticles. A modified larval key is provided for known species of this genus.
Investigating the Relationship between Lightning and Mesocyclonic Rotation in Supercell Thunderstorms
Authors: Stough Sarah M. , Carey Lawrence D. , Schultz Christopher J. , Bitzer Phillip M. 
Published: November 2017

Abstract
Relationships between lightning and lightning jumps and physical updraft properties are frequently observed and generally understood. However, a more intensive characterization of how lightning relates to traditional radar-based metrics of storm intensity may provide further operational utility. This study addresses the supercell storm mode because of the intrinsic relationship between a supercell’s characteristic rotating updraft–downdraft couplet, or mesocyclone, and its prolific ability to produce severe weather. Lightning and radar measurements of a diverse sample of 19 supercell thunderstorms were used to assess the conceptual model that lightning and the mesocyclone may be linked by the updraft’s role in the formation and enhancement of each. Analysis of early stages of supercell development showed that the initial lightning jump occurred prior to the time of mesocyclogenesis inferred from three methods by median values of 5–10 min. Comparison between lightning jumps and subsequent increases in mesocyclonic rotation indicated that lightning can also be used to infer or confirm imminent strengthening or reintensification of the mesocyclone. Stronger relationships emerged in supercells that exhibited more robust updrafts, in which 85% of lightning jumps were associated with at least one increase in rotation and 77% of observed increases in rotation were temporally associated with a lightning jump. Preliminary results from analysis of the relationship between lightning jumps and intensification of the low-level mesocyclone in tornadic supercells also offer motivation for the future analysis of lightning data with respect to downdraft-related processes.