bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multi-country assessment of factors related to smallholder food security in varying rainfall conditions
Authors: Niles Meredith T. , Brown Molly E. 
Published: November 2017

AbstractGiven that smallholder farmers are frequently food insecure and rely significantly on rain-fed agriculture, it is critical to examine climate variability and food insecurity. We utilize data from smallholder farmer surveys from 12 countries with 30 years of rainfall data to examine how rainfall variability and household resources are correlated with food security. We find that on average, households that experienced a drier than average year are 3.81 months food insecure, while households within a normal range of rainfall were 3.67 months food insecure, and wetter than average households were 2.86 months food insecure. Reduced odds of food insecurity is associated with agricultural inputs, ownership of livestock, water use efficiency, financial services, and participation in a group. However, in drier than average households, financial services as compared to agricultural inputs and agroecological practices have a greater prevalence of reduced instances of food insecurity, while agricultural inputs are more common for reduced food insecurity in wetter than average households. Only the use of fertilizer consistently results in reduced odds of food insecurity across all households regardless of rainfall, demonstrating that one-size fits all approaches to food security interventions are likely ineffective, and place-specific interventions considering climatic factors are critically important.
Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay
Authors: Reslová Nikol , Škorpíková Lucie , Slaný Michal , Pozio Edoardo , Kašný Martin 
Published: November 2017

AbstractHigh resolution melting analysis (HRMA) is a single-tube method, which can be carried out rapidly as an additional step following real-time quantitative PCR (qPCR). The method enables the differentiation of genetic variation (down to single nucleotide polymorphisms) in amplified DNA fragments without sequencing. HRMA has previously been adopted to determine variability in the amplified genes of a number of organisms. However, only one work to date has focused on pathogenic parasites–nematodes from the genus Trichinella. In this study, we employed a qPCR-HRMA assay specifically targeting two sequential gene fragments–cytochrome c oxidase subunit I (COI) and expansion segment V (ESV), in order to differentiate 37 single L1 muscle larvae samples of eight Trichinella species. We show that qPCR-HRMA based on the mitochondrial COI gene allows differentiation between the sequences of PCR products of the same length. This simple, rapid and reliable method can be used to identify at the species level single larvae of eight Trichinella taxa.
Current data on extremities chronic osteomyelitis in southwest China: epidemiology, microbiology and therapeutic consequences
Authors: Wang Xiaohua , Yu Shengpeng , Sun Dong , Fu Jingshu , Wang Shulin , Huang Ke , Xie Zhao 
Published: November 2017

AbstractThe current study was designed to explore the epidemiology of extremities chronic osteomyelitis, its prognosis and the complications of the treatment methods being used in southwest China. The data from osteomyelitis patients treated at the Department of Orthopaedics, Southwest Hospital, China between May 2011 and September 2016 were collected and analysed. The study comprised 503 admitted patients, of which 416 males and 87 were females, with an average age of 40.15 ± 5.64 years. Approximately 356 cases were followed for more than 18 months; the average bone union time was 6.24 ± 0.76 months in 94.1% (335) patients, and infections were almost controlled in 93.8% patients. The rate of infection control with the induced membrane technique was higher than with the I-stage free bone graft. Iliac infection was the main complication of the induced membrane technique, and impaired joint activity was the main complication of I-stage free bone grafts. In southwest China, the incidence of haematogenous osteomyelitis, caused mainly by Staphylococcus aureus, remains very high. The speed of bone defect repair and the rate of infection control with the induced membrane technique were superior to those of I-stage free bone grafts. Internal fixation should be given priority because it offers reduced complications with no increase in the recurrence of infection.
Population pharmacokinetics of remifentanil in critically ill patients receiving extracorporeal membrane oxygenation
Authors: Yang Seungwon , Noh Hayeon , Hahn Jongsung , Jin Byung Hak , Min Kyoung Lok , Bae Soo Kyung , Kim Jiseon , Park Min Soo , Hong Taegon , Wi Jin , Chang Min Jung 
Published: November 2017

AbstractExtracorporeal membrane oxygenation (ECMO) is associated with pharmacokinetic (PK) changes of drugs. It presents considerable challenges to providing optimal dosing regimens for patients receiving ECMO. We aimed to describe the population PK of remifentanil in critically ill adult patients receiving venoartrial extracorporeal membrane oxygenation (VA-ECMO) and to identify determinants associated with altered remifentanil concentrations. The population PK model of remifentanil was developed using nonlinear mixed effects modelling (NONMEM). Fifteen adult patients who received a continuous infusion of remifentanil during VA-ECMO participated in the study. The PK of remifentanil was best described by a one-compartment model with additive and proportional residual errors. Remifentanil concentrations were affected by sex and ECMO pump speed. The final PK model included the effect of sex and ECMO pump speed on clearance is developed as followed: clearance (L/h) = 366 × 0.502sex × (ECMO pump speed/2350)2.04 and volume (L) = 41. Remifentanil volume and clearance were increased in adult patients on VA-ECMO compared with previously reported patients not on ECMO. We suggest that clinicians should consider an increased remifentanil dosing to achieve the desired level of sedation and provide a dosing regimen according to sex and ECMO pump speed.
The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence
Authors: Olszak Tomasz , Shneider Mikhail M. , Latka Agnieszka , Maciejewska Barbara , Browning Christopher , Sycheva Lada V. , Cornelissen Anneleen , Danis-Wlodarczyk Katarzyna , Senchenkova Sofya N. , Shashkov Alexander S. , Gula Grzegorz , Arabski Michal , Wasik Slawomir , Miroshnikov Konstantin A. , Lavigne Rob , Leiman Petr G. , Knirel Yuriy A. , Drulis-Kawa Zuzanna 
Published: November 2017

AbstractPseudomonas phage LKA1 of the subfamily Autographivirinae encodes a tailspike protein (LKA1gp49) which binds and cleaves B-band LPS (O-specific antigen, OSA) of Pseudomonas aeruginosa PAO1. The crystal structure of LKA1gp49 catalytic domain consists of a beta-helix, an insertion domain and a C-terminal discoidin-like domain. The putative substrate binding and processing site is located on the face of the beta-helix whereas the C-terminal domain is likely involved in carbohydrates binding. NMR spectroscopy and mass spectrometry analyses of degraded LPS (OSA) fragments show an O5 serotype-specific polysaccharide lyase specificity. LKA1gp49 reduces virulence in an in vivo Galleria mellonella infection model and sensitizes P. aeruginosa to serum complement activity. This enzyme causes biofilm degradation and does not affect the activity of ciprofloxacin and gentamicin. This is the first comprehensive report on LPS-degrading lyase derived from a Pseudomonas phage. Biological properties reveal a potential towards its applications in antimicrobial design and as a microbiological or biotechnological tool.
A novel peptidic inhibitor derived from Streptococcus cristatus ArcA attenuates virulence potential of Porphyromonas gingivalis
Authors: Ho Meng-Hsuan , Lamont Richard J. , Xie Hua 
Published: November 2017

AbstractPeriodontitis is a global health problem and the 6th most common infectious disease worldwide. Porphyromonas gingivalis is considered a keystone pathogen in the disease and is capable of elevating the virulence potential of the periodontal microbial community. Strategies that interfere with P. gingivalis colonization and expression of virulence factor are therefore attractive approaches for preventing and treating periodontitis. We have previously reported that an 11-mer peptide (SAPP) derived from Streptococcus cristatus arginine deiminase (ArcA) was able to repress the expression and production of several well-known P. gingivalis virulence factors including fimbrial proteins and gingipains. Herein we expand and develop these studies to ascertain the impact of this peptide on phenotypic properties of P. gingivalis related to virulence potential. We found that growth rate was not altered by exposure of P. gingivalis to SAPP, while monospecies and heterotypic biofilm formation, and invasion of oral epithelial cells were inhibited. Additionally, SAPP was able to impinge the ability of P. gingivalis to dysregulate innate immunity by repressing gingipain-associated degradation of interleukin-8 (IL8). Hence, SAPP has characteristics that could be exploited for the manipulation of P. gingivalis levels in oral communities and preventing realization of virulence potential.
The Neural Basis of Changing Social Norms through Persuasion
Authors: Yomogida Yukihito , Matsumoto Madoka , Aoki Ryuta , Sugiura Ayaka , Phillips Adam N. , Matsumoto Kenji 
Published: November 2017

AbstractSocial norms regulate behavior, and changes in norms have a great impact on society. In most modern societies, norms change through interpersonal communication and persuasive messages found in media. Here, we examined the neural basis of persuasion-induced changes in attitude toward and away from norms using fMRI. We measured brain activity while human participants were exposed to persuasive messages directed toward specific norms. Persuasion directed toward social norms specifically activated a set of brain regions including temporal poles, temporo-parietal junction, and medial prefrontal cortex. Beyond these regions, when successful, persuasion away from an accepted norm specifically recruited the left middle temporal and supramarginal gyri. Furthermore, in combination with data from a separate attitude-rating task, we found that left supramarginal gyrus activity represented participant attitude toward norms and tracked the persuasion-induced attitude changes that were away from agreement.
Stable Hydrothermal Waves at Steady State Evaporating Droplet Surface
Authors: Zhong Xin , Duan Fei 
Published: November 2017

AbstractStable hydrothermal waves (HTWs) are found at a sessile ethanol droplet surface under the steady state evaporation. It is different from those which greatly decrease with the evaporation time in the transient droplet drying process. This study removes the possible effect of shrinking droplet on HTWs. An analysis of the dimensionless numbers indicates the increasingly enhanced role of thermocapillary instabilities upon raising the substrate temperature. The onset HTWs can be continuously maintained under the steady state evaporation conditions. Interestingly, the stable number of HTWs follows a linear fitting with the dimensionless factor incorporating the normalized temperature difference between the substrate and the surroundings and the droplet aspect ratio. The temperature heterogeneity of HTWs is intensified by increasing the substrate temperature. The stable HTWs exhibit the “one source-to-one sink” propagation at lower substrate temperatures. However, such directional traveling, normally presented in the transient HTWs in the drying droplet, is changed at the higher substrate temperatures due to the newly emerged sources and sinks under the steady state conditions.
Low-temperature fabrication of an HfO2 passivation layer for amorphous indium–gallium–zinc oxide thin film transistors using a solution process
Authors: Hong Seonghwan , Park Sung Pyo , Kim Yeong-gyu , Kang Byung Ha , Na Jae Won , Kim Hyun Jae 
Published: November 2017

AbstractWe report low-temperature solution processing of hafnium oxide (HfO2) passivation layers for amorphous indium–gallium–zinc oxide (a-IGZO) thin-film transistors (TFTs). At 150 °C, the hafnium chloride (HfCl4) precursor readily hydrolyzed in deionized (DI) water and transformed into an HfO2 film. The fabricated HfO2 passivation layer prevented any interaction between the back surface of an a-IGZO TFT and ambient gas. Moreover, diffused Hf4+ in the back-channel layer of the a-IGZO TFT reduced the oxygen vacancy, which is the origin of the electrical instability in a-IGZO TFTs. Consequently, the a-IGZO TFT with the HfO2 passivation layer exhibited improved stability, showing a decrease in the threshold voltage shift from 4.83 to 1.68 V under a positive bias stress test conducted over 10,000 s.
Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4
Authors: Konuma Tsuyoshi , Yu Di , Zhao Chengcheng , Ju Ying , Sharma Rajal , Ren Chunyan , Zhang Qiang , Zhou Ming-Ming , Zeng Lei 
Published: November 2017

AbstractJumonji domain-containing protein 6 (JMJD6) is a member of the Jumonji C family of Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases. It possesses unique bi-functional oxygenase activities, acting as both an arginine demethylase and a lysyl-hydroxylase. JMJD6 has been reported to be over-expressed in oral, breast, lung, and colon cancers and plays important roles in regulation of transcription through interactions with transcription regulator BRD4, histones, U2AF65, Luc7L3, and SRSF11. Here, we report a structural mechanism revealed by NMR of JMJD6 recognition by the extraterminal (ET) domain of BRD4 in that a JMJD6 peptide (Lys84-Asn96) adapts an α-helix when bound to the ET domain. This intermolecular recognition is established through JMJD6 interactions with the conserved hydrophobic core of the ET domain, and reinforced by electrostatic interactions of JMJD6 with residues in the inter-helical α1-α2 loop of the ET domain. Notably, this mode of ligand recognition is different from that of ET domain recognition of NSD3, LANA of herpesvirus, and integrase of MLV, which involves formation of an intermolecular amphipathic two- or three- strand antiparallel β sheet. Furthermore, we demonstrate that the association between the BRD4 ET domain and JMJD6 likely requires a protein conformational change induced by single-stranded RNA binding.
Interventionen zur präoperativen Unterstützung geriatrischer Patienten mit Frailty
Authors: Kiselev Jörn 
Published: November 2017

ZusammenfassungDer stetig zu beobachtende demografische Wandel geht mit veränderten Anforderungen an die Patientenbetreuung einher. Ältere Patienten weisen häufig einen erhöhten Behandlungsbedarf und eine höhere Komplexität des klinischen Gesamtbildes auf. Dieser Umstand ist – abgesehen von der Routineversorgung – besonders dann zu beachten, wenn eine angedachte Intervention zu schweren und potenziell lebensbedrohlichen Komplikationen führen kann.
Frailty: Prävalenz bei 70- bis 79-Jährigen in Deutschland (populationsbasierter Ansatz)
Authors: Fuchs Judith , Busch Markus , Scheidt-Nave Christa 
Published: November 2017

ZusammenfassungIn allen Ländern steigt die Lebenserwartung und viele ältere Menschen verbringen mehr Zeit bei guter Gesundheit. Allerdings leiden Ältere auch unter Gebrechlichkeit (engl. frailty), die als Risikofaktor für Erkrankungen, Behinderungen oder Stürze gilt. Im Rahmen der „Studie zur Gesundheit Erwachsener in Deutschland“ (DEGS) 2008 – 2011 wurden Interviews, Untersuchungen und Tests durchgeführt. Für die Auswertungen wurden Daten von 1110 zu Hause lebenden DEGS1-Teilnehmenden im Alter von 70 bis 79 Jahren analysiert. Körperliche Gebrechlichkeit wurde definiert als das Vorliegen von drei oder mehr der folgenden Kriterien: selbst berichtete Erschöpfung, reduzierte Greifkraft, verlangsamte Gehgeschwindigkeit, geringe körperliche Aktivität und ein niedriger Body-Mass-Index; beim Vorliegen ein oder zwei der Kriterien wird von einer Vorstufe der Gebrechlichkeit (pre-frailty) ausgegangen. Bei Männern wurden 36,6% (Konfidenzintervall KI 32,2 – 41,2) als pre-frail, 2,5% (KI 1,4 – 4,4) als gebrechlich eingestuft; bei Frauen wurden 41,8% (KI 37,3 – 46,5) als pre-frail und 2,9% (KI 1,9 – 4,5) als gebrechlich eingestuft. Gebrechliche Personen geben signifikant häufiger Polypharmazie und Mehrfach-Stürze an, beziehen Leistungen aus der Pflegeversicherung, haben eine amtlich anerkannte Schwerbehinderung und geringere soziale Unterstützung. Diese bevölkerungsrepräsentativen Ergebnisse tragen zur Identifikation von gebrechlichen Menschen mit einem erhöhten Risiko für gesundheitliche Folgen bei. Entsprechende Prognosemodelle sollten in der operativen Anästhesiologie für die verschiedenen Versorgungsbereiche weiterentwickelt werden.
Zervikale Plexusblockaden
Authors: Seidel Ronald 
Published: November 2017

ZusammenfassungEine vollständige Blockade des Plexus cervicalis ist weder erwünscht noch ausreichend für eine chirurgische Anästhesie in der vorderen Halsregion. Supplementierungen durch Opioide oder topische Lokalanästhetika sind daher häufig. Die Blockade beteiligter Hirnnerven und des Truncus sympathicus verbessert möglicherweise die Anästhesiequalität, führt aber auch zu typischen Nebenwirkungen. Dieser Beitrag liefert anatomisches Hintergrundwissen und stellt Indikationen vor – auch außerhalb der Karotischirurgie.
Diagnostik und Therapie von Irisläsionen
Authors: Mor Joel , Koch Konrad , Heindl Ludwig 
Published: November 2017

ZusammenfassungTeil des klinisch-ophthalmologischen Alltags ist die korrekte Diagnose tumoröser Irisläsionen. Insbesondere die Differenzialdiagnose und die Bewertung der Dignität anhand der Morphologie gestalten sich dabei oft schwierig. Dieser Beitrag gibt einen Überblick über das breite Spektrum von Iristumoren einschließlich empfohlener Diagnostik- und Therapiemodalitäten.
Funktionelle und anatomische Ergebnisse der primären und sekundären Internal-Limiting-Membrane-Transplantation zur Behandlung von großen und voroperierten Makulaforamina
Authors: Hess Jelka , Michels Stephan , Becker Matthias 
Published: November 2017

Zusammenfassung
Hintergrund Der Goldstandard beim durchgreifenden Makulaforamen (MF) ist die Vitrektomie (VE) mit Peeling der Membrana limitans interna (ILM), Gastamponade und postoperativer Kopftieflagerung. Größere MF (> 400 µm) und voroperierte Makulaforamina bleiben weiterhin schwierig behandelbar. Kürzlich wurden neue ILM-Transplantationstechniken (ILM-TX) mit akzeptablen Resultaten entwickelt. Diese greifen auf verschiedene Verschlussmechanismen zurück: ILM als Leitschiene für Migration des neuronalen Gewebes beim gestielten ILM-Flap oder Kontraktion der MF-Ränder durch Schrumpfung eines ILM-Pfropfens.
Patienten/Material und Methoden Diese retrospektive Studie evaluiert die funktionellen und anatomischen Ergebnisse nach ILM-TX bei großen MF und nach Vitrektomie mit ILM-Peeling bei persistierenden MF. Für die großen MF (Gruppe 1) wurde der gestielte ILM-Flap oder die freie Faltung des ILM-TX in das MF angewandt. Persistierende MF nach VE mit ILM-Peeling (Gruppe 2) wurden mittels Faltung eines freien ILM-TX in das Foramen behandelt. Bei allen Augen erfolgte die ILM-TX unter Perfluorocarbon-Liquid-Schutz und Silikonöltamponade.
Ergebnisse In der Gruppe 1 (6 Augen) erhielten 3 Augen eine freie ILM-TX in das Foramen und 3 Augen erhielten eine gestielte ILM-TX. Die durchschnittliche bestkorrigierte Sehschärfe (BCVA, angegeben in LogMar) lag präoperativ bei 1,18 ± 0,54. Die präoperative MF-Größe belief sich auf 681 ± 106 µm mit einem Photorezeptorbanddefekt (PRD) von 1829 ± 474 µm. In 5 von 6 Augen konnte ein Verschluss des MF erzielt werden (83%). Nach einem Follow-up von durchschnittlich 9,3 ± 5,1 Monaten fand sich eine BCVA von 0,83 ± 0,31 nach freiem und 0,95 ± 0,79 nach gestieltem ILM-TX. Der mittlere PRD reduzierte sich auf 1781 ± 713 µm bei den freien und auf 1148 ± 378 µm bei den gestielten ILM-TX. In Gruppe 2 (7 Augen) mit persistierenden MF nach Standardvitrektomie und ILM-Peeling lag der Visus vor der sekundären ILM-TX bei 1,05 ± 0,41. Die initiale MF-Größe betrug 563 ± 183 µm. Der durchschnittliche PRD betrug 2012 ± 718 µm vor der sekundären ILM-TX. Nach der sekundären ILM-TX, nach einem mittleren Follow-up von 9,6 ± 4,1 Monaten, zeigten 6 von 7 Augen einen Verschluss des MF (86%). Die Sehschärfe betrug dabei 0,53 ± 0,34 und der PRD reduzierte sich auf 843 ± 291 µm.
Schlussfolgerung ILM-TX führt bei großen MF oder persistierenden MF nach VE mit ILM-Peeling meist zum Verschluss des MF. Die funktionellen Ergebnisse zeigen eine Stabilisierung und manchmal sogar eine Verbesserung.
Evolving academic culture to meet societal needs
Authors: Wowk Kateryna , McKinney Larry , Muller-Karger Frank , Moll Russell , Avery Susan , Escobar-Briones Elva , Yoskowitz David , McLaughlin Richard 
Published: November 2017

Abstract
Given today’s complex societal challenges, academia should work better with government, industry and others in offering innovative solutions that benefit our society, economy and environment. Researchers across disciplines must work together and with decision-makers to understand how science can have better on-the-ground impacts toward longer-term, resilient societal outcomes. This includes, for example, by working with end-users in problem formation and throughout research projects to ensure decision-making needs are being met, and by linking physical science to additional fields like economics, risk communication or psychology. However, persistent barriers to collaborating across disciplines and with external decision-makers remain. Despite decades of studies highlighting the need for interdisciplinary research and science for decision-making, academic institutions are still not structured to facilitate or reward such collaboration. A group of researchers and educators used a mixed-methods approach to consider the knowledge base on interdisciplinary research and evidence-based policymaking, as well as their own experiences, and formed targeted and actionable recommendations that can help academia overcome these barriers. Their recommendations, specifically targeted to administrators, institutional leads, individual researchers, and research funders, align to three categories: define the role of academia in linking to policy; incorporate nontraditional standards in evaluating success; and build trust while drawing the line between knowledge dissemination and activism. By implementing the following recommendations, academics can foster the culture change that is needed to promote interdisciplinarity, strengthen the impact of their work and help society address urgent and multi-faceted problems.
Hydrophobicity of proteins and nanostructured solutes is governed by topographical and chemical context
Authors: Xi Erte , Venkateshwaran Vasudevan , Li Lijuan , Rego Nicholas , Patel Amish J. , Garde Shekhar 
Published: November 2017

Significance
Numerous biological self-assembly processes, from protein folding to molecular recognition, are driven by hydrophobic interactions, yet characterizing hydrophobicity at the nanoscale has remained a major challenge, because it requires understanding of the strength of protein–water interactions and the ease with which they can be disrupted. Water near a protein responds to its chemistry and topography in a manner that is collective and complex and cannot be captured by commonly used surface area models or hydropathy scales. We demonstrate that water density fluctuations near proteins can characterize protein hydrophobicity and reveal its dependence on curvature and chemical patterns at the nanoscale. Our approach opens new avenues for understanding and efficient characterization of biomolecular interactions.