bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expressions of SH3BP5, LMO3, and SNAP25 in diffuse large B‐cell lymphoma cells and their association with clinical features
Authors: Kobayashi Kyoko , Yamaguchi Motoko , Miyazaki Kana , Imai Hiroshi , Yokoe Kaori , Ono Ryoichi , Nosaka Tetsuya , Katayama Naoyuki 
Published: May 2016

AbstractDiffuse large B‐cell lymphoma (DLBCL) is clinicopathologically and genetically heterogeneous with variable clinical outcomes. We previously identified signature genes overexpressed in CD5‐positive (CD5+) DLBCL, which is a poor prognostic subgroup of DLBCL. To elucidate the clinical significance of the protein expression of the signature genes overexpressed in CD5+ DLBCL with regard to all DLBCL, not otherwise specified (NOS), 10 genes (SH3BP5, LMO3, SNAP25, SYT5, SV2C, CABP1, FGF1, FGFR2, NEUROD1, and SYN2) were selected and examined immunohistochemically with samples from 28 patients with DLBCL, NOS. Only three protein expressions, SH3BP5, LMO3, and SNAP25, were detected in DLBCL cells and then analyzed further with samples from 187 patients with DLBCL, NOS. The SH3BP5, LMO3, and SNAP25 proteins were expressed in 60% (103/173), 34% (59/175), and 46% (77/168) of DLBCL patients, respectively. These protein expressions were associated with CD5 expression, and only SH3BP5 was frequently expressed in activated B‐cell‐like DLBCL (P = 0.046). Compared to the SH3BP5‐negative group, the SH3BP5+ group was correlated with elderly onset (>60 years, P = 0.0096) and advanced‐stage disease (stage III/IV, P = 0.037). The LMO3+ group showed a worse performance status (>1, P = 0.0004). The SH3BP5+ group and the LMO3+ group had significantly worse overall survival than the negative groups (P = 0.030, 0.034; respectively) for the entire group. In a subgroup analysis of patients treated with rituximab‐containing chemotherapy, there was no significant difference between groups. To the best of our knowledge, this is the first report showing the protein expressions of SH3BP5, LMO3, and SNAP25 in DLBCL cells and their clinical significance in patients with DLBCL. The SH3BP5 and LMO3 protein expressions are associated with the baseline clinical characteristics of DLBCL.
Reduction in squamous cell carcinomas in mouse skin by dietary zinc supplementation
Authors: Sun Jin , Shen Rulong , Schrock Morgan S. , Liu James , Pan Xueliang , Quimby Donald , Zanesi Nicola , Druck Teresa , Fong Louise Y. , Huebner Kay 
Published: May 2016

AbstractInadequate dietary Zn consumption increases susceptibility to esophageal and other cancers in humans and model organisms. Since Zn supplementation can prevent cancers in rodent squamous cell carcinoma (SCC) models, we were interested in determining if it could have a preventive effect in a rodent skin cancer model, as a preclinical basis for considering a role for Zn in prevention of human nonmelanoma skin cancers, the most frequent cancers in humans. We used the 7,12‐dimethyl benzanthracene carcinogen/phorbol myristate acetate tumor promoter treatment method to induce skin tumors in Zn‐sufficient wild‐type and Fhit (human or mouse protein) knockout mice. Fhit protein expression is lost in >50% of human cancers, including skin SCCs, and Fhit‐deficient mice show increased sensitivity to carcinogen induction of tumors. We hypothesized that: (1) the skin cancer burdens would be reduced by Zn supplementation; (2) Fhit−/−(Fhit, murine fragile histidine triad gene) mice would show increased susceptibility to skin tumor induction versus wild‐type mice. 30 weeks after initiating treatment, the tumor burden was increased ~2‐fold in Fhit−/− versus wild‐type mice (16.2 versus 7.6 tumors, P < 0.001); Zn supplementation significantly reduced tumor burdens in Fhit−/− mice (males and females combined, 16.2 unsupplemented versus 10.3 supplemented, P = 0.001). Most importantly, the SCC burden was reduced after Zn supplementation in both strains and genders of mice, most significantly in the wild‐type males (P = 0.035). Although the mechanism(s) of action of Zn supplementation in skin tumor prevention is not known in detail, the Zn‐supplemented tumors showed evidence of reduced DNA damage and some cohorts showed reduced inflammation scores. The results suggest that mild Zn supplementation should be tested for prevention of skin cancer in high‐risk human cohorts.
In‐Phase Assembly of Slim DNA Lattices with Small Circular DNA Motifs via Short Connections of 11 and 16 Base Pairs
Authors: Wang Meng , Guo Xin , Jiang Chuan , Wang Xuemei , Xiao Shou‐Jun 
Published: May 2016

AbstractTwo kinds of stable motif were constructed: SAE (semi‐crossover, antiparallel, even half‐turns) tile from one small circular DNA molecule (42 or 64 nt) and two linear oligonucleotides; and DAE (double‐crossover, antiparallel, even half‐turns) tile from one small circular DNA molecule (42 or 64 nt) and four linear oligonucleotides. With the SAE tiles, in‐phase assembly of SAE–E (SAE tiles with even half‐turns as connections (–E)) with the shortest –E of 11 base pairs (bp) generated homogeneous nanotubes with an average length of over 14 μm and a diameter of 16–20 nm; with the DAE tiles, in‐phase assembly of DAE–O (DAE tiles with odd half‐turns as connections (–O)) with the shortest –O of 16 bp produced slim monolayer nanoyarns (25–30 nm wide), nanoscarfs (100–300 nm wide), and nanoribbons (∼100 nm wide). Interestingly, a phenomenon we term “knitting nanoyarns” into nanoscarfs was observed. Finally a curvature mechanism according to the ring rotation directions is suggested to explain the formation of nanotubes, wavy nanoyarns, nanoscarfs, and nanoribbons.
Clinical outcome and paravalvular leakage of the new balloon‐expandable Edwards Sapien 3 valve in comparison to its predecessor model (Edwards Sapien XT) in patients undergoing transfemoral aortic valve replacement
Authors: Bocksch Wolfgang , Grossmann Benita , Geisler Tobias , Steeg Martin , Droppa Michal , Jorbenadze Rezo , Haap Michael , Gawaz Meinrad , Fateh‐Moghadam Suzanne 
Published: May 2016

ObjectivesThe aim of this study was to compare the 30‐day procedural, clinical and echocardiographic outcome of the new balloon‐expandable Edwards Sapien 3 (ES3) valve with the Edwards Sapien XT (ESXT). Background: Post‐implant paravalvular leaks (PVL) after transfemoral aortic valve replacement (TAVR) resulting in residual aortic regurgitation (AR) are a major limitation for long term outcome. New TAVR‐devices have to eliminate this problem.MethodsTransfemoral TAVR was performed in 209 consecutive intermediate‐high‐risk surgical patients (pts) with symptomatic aortic stenosis (ESXT n = 102, ES3 n = 107). Transthoracic echocardiography (TTE) and 3‐dimensional computed tomography were used for valve size selection. Primary endpoint of the study was none/trace AR derived by TTE 30‐days after TAVR.ResultsAll pts underwent successfully TAVR with a combined device success of 100/102 (99%) in ESXT and 107/107 (100%) in ES3 pts. Fluoroscopy time (ESXT 11.8 ± 0.5 min vs. ES3 10.0 ± 0.5 min, P = 0.003) and contrast (ESXT 188.9 ± 5.6 mL vs. ES3 170.4 ± 4.7 mL, P = 0.04) were significantly lower in ES3 patients. 30‐day clinical events did not differ. Transvalvular mean pressure gradients were significantly reduced to 7.4 ± 0.8 mmHg after ESXT and to 10.1± 0.4 mmHg after ES3 implantation. After 30 days none/trace AR was found in 34.3% (n = 35) of all ESXT pts in contrast to 89.7% (n = 96) of all ES3 patients. Moderate‐to‐severe AR was found rarely (ESXT 2.9% vs. ES3 0%, P = 0.073).ConclusionsAlthough there was no significant difference in 30 day mortality, the newer ES3 valve reduced significantly residual paravalvular leakage. © 2016 Wiley Periodicals, Inc.
Guidewire and microcatheter utilization patterns during antegrade wire escalation in chronic total occlusion percutaneous coronary intervention: Insights from a contemporary multicenter registry
Authors: Karatasakis Aris , Tarar Muhammad Nauman J. , Karmpaliotis Dimitri , Alaswad Khaldoon , Yeh Robert W. , Jaffer Farouc A. , Wyman R. Michael , Lombardi William L. , Grantham J. Aaron , Kandzari David E. , Lembo Nicholas J. , Moses Jeffrey W. , Kirtane Ajay J. , Parikh Manish , Garcia Santiago , Doing Anthony , Pershad Ashish , Shah Alpesh , Patel Mitul , Bahadorani John , Shoultz Charles A. , Danek Barbara A. , Thompson Craig A. , Banerjee Subhash , Brilakis Emmanouil S. 
Published: May 2016

ObjectivesWe sought to describe contemporary guidewire and microcatheter utilization for antegrade wire escalation (AWE) during chronic total occlusion (CTO) percutaneous coronary intervention (PCI).BackgroundEquipment utilization for AWE has been variable and evolving over time.MethodsWe examined device utilization during 694 AWE attempts in 679 patients performed at 15 experienced US centers between May 2012 and April 2015.ResultsMean age was 65.6 ± 9.7 years, and 85% of the patients were men. Successful wiring occurred in 436 AWE attempts (63%). Final technical and procedural success was 91% and 89%, respectively. The mean number of guidewire types used for AWE was 2.2 ± 1.4. The most frequently used guidewire types were the Pilot 200 (Abbott Vascular, 56% of AWE procedures), Fielder XT (Asahi Intecc, 45%), and the Confianza Pro 12 (Asahi Intecc, 28%). The same guidewires were the ones that most commonly crossed the occlusion: Pilot 200 (36% of successful AWE crossings), Fielder XT (20%), and Confianza Pro 12 (11%). A microcatheter or over‐the‐wire balloon was used for 81% of AWE attempts; the Corsair microcatheter (Asahi Intecc) was the most commonly used (44%). No significant association was found between guidewire type and incidence of major adverse cardiac events (MACE).ConclusionsOur contemporary, multicenter CTO PCI registry demonstrates that the most commonly used wires for AWE are polymer‐jacketed guidewires. “Stiff” and polymer‐jacketed guidewires appear to provide high crossing rates without an increase in MACE or perforation, and may thus be considered for upfront use. © 2016 Wiley Periodicals, Inc.
Complex bifurcation lesions: Randomized comparison of a fully bioresorbable modified t stenting strategy versus bifurcation reconstruction with a dedicated self‐expanding stent in combination with bioresorbable scaffolds, an OCT study: Rationale and design of the COBRA II trial
Authors: Bennett J. , Adriaenssens T. , Desmet W. , Dubois C. 
Published: May 2016

ObjectiveThere is an ongoing controversy regarding the efficacy and safety of different percutaneous stenting techniques for coronary bifurcation lesions needing >1 stent. The promise of safe vessel restoration with bioresorbable scaffolds (BRS) may not be transferable to complex double BRS bifurcation techniques, and permanent metallic scaffolding of the bifurcation core may be needed. We identified modified‐T stenting as the most promising fully bioresorbable 2‐stent strategy in a preclinical setting. The objective of this study is to assess acute performance and compare long‐term vessel healing with this strategy, versus an approach combining BRS with a dedicated metallic drug‐eluting bifurcation stent.Study designIn a single center, 60 consecutive patients with true and complex coronary bifurcation lesions will be randomly assigned to treatment with the dedicated self‐expanding Axxess™ biolimus‐eluting bifurcation stent in the proximal main vessel and additional Absorb™ everolimus‐eluting BRS in the branches versus a modified T technique using Absorb™ only. Angiography and optical coherence tomography (OCT) will be performed immediately after implantation and at 30 months, and clinical follow‐up is foreseen up to 5 years after implantation. The primary endpoint is the change in minimal luminal area assessed with OCT from baseline to 30 months in pre‐specified bifurcation segments.ConclusionTo date the use of Absorb™ BRS in complex coronary bifurcations has not been evaluated in a randomized clinical trial setting. The COBRA II study will examine the role and safety of a double BRS strategy in coronary bifurcations, alone or in combination with a metallic dedicated bifurcation device. © 2016 Wiley Periodicals, Inc.
Total Synthesis of Δ12‐Prostaglandin J3: Evolution of Synthetic Strategies to a Streamlined Process
Authors: Nicolaou K. C. , Pulukuri Kiran Kumar , Yu Ruocheng , Rigol Stephan , Heretsch Philipp , Grove Charles I. , Hale Christopher R. H. , ElMarrouni Abdelatif 
Published: May 2016

AbstractThe total synthesis of Δ12‐prostaglandin J3 (Δ12‐PGJ3, 1), a reported leukemia stem cell ablator, through a number of strategies and tactics is described. The signature cross‐conjugated dienone structural motif of 1 was forged by an aldol reaction/dehydration sequence from key building blocks enone 13 and aldehyde 14, whose lone stereocenters were generated by an asymmetric Tsuji–Trost reaction and an asymmetric Mukaiyama aldol reaction, respectively. During this program, a substituent‐governed regioselectivity pattern for the Rh‐catalyzed C−H functionalization of cyclopentenes and related olefins was discovered. The evolution of the synthesis of 1 from the original strategy to the final streamlined process proceeded through improvements in the construction of both fragments 13 and 14, exploration of the chemistry of the hitherto underutilized chiral lactone synthon 57, and a diastereoselective alkylation of a cyclopentenone intermediate. The described chemistry sets the stage for large‐scale production of Δ12‐PGJ3 and designed analogues for further biological and pharmacological studies.
Disparities in cancer treatment among patients infected with the human immunodeficiency virus
Authors: Suneja Gita , Lin Chun Chieh , Simard Edgar P. , Han Xuesong , Engels Eric A. , Jemal Ahmedin 
Published: May 2016

BACKGROUNDPatients with cancer who are infected with the human immunodeficiency virus (HIV) are less likely to receive cancer treatment compared with HIV‐uninfected individuals. However, to the authors' knowledge, the impact of insurance status and comorbidities is unknown.METHODSData from the National Cancer Data Base were used to study nonelderly adults diagnosed with several common cancers from 2003 to 2011. Cancer treatment was defined as chemotherapy, surgery, radiotherapy, or any combination during the first course of treatment. Multivariate logistic regression was used to examine associations between HIV status and lack of cancer treatment, and identify predictors for lack of treatment among HIV‐infected patients.RESULTSA total of 10,265 HIV‐infected and 2,219,232 HIV‐uninfected cases were included. In multivariate analysis, HIV‐infected patients with cancer were found to be more likely to lack cancer treatment for cancers of the head and neck (adjusted odds ratio [aOR], 1.48; 95% confidence interval [95% CI], 1.09‐2.01), upper gastrointestinal tract (aOR, 2.62; 95% CI, 2.04‐3.37), colorectum (aOR, 1.70; 95% CI, 1.17‐2.48), lung (aOR, 2.46; 95% CI, 2.19‐2.76), breast (aOR, 2.14; 95% CI, 1.16‐3.98), cervix (aOR, 2.81; 95% CI, 1.77‐4.45), prostate (aOR, 2.16; 95% CI, 1.69‐2.76), Hodgkin lymphoma (aOR, 1.92; 95% CI, 1.66‐2.22), and diffuse large B‐cell lymphoma (aOR, 1.82; 95% CI, 1.65‐2.00). Predictors of a lack of cancer treatment among HIV‐infected individuals varied by tumor type (solid tumor vs lymphoma), but black race and a lack of private insurance were found to be predictors for both groups.CONCLUSIONSIn the United States, HIV‐infected patients with cancer appear to be less likely to receive cancer treatment regardless of insurance and comorbidities. To the authors' knowledge, the current study is the largest study of cancer treatment in HIV‐infected patients with cancer in the United States and provides evidence of cancer treatment disparities even after controlling for differences with regard to insurance status and comorbidities. Further work should focus on addressing differential cancer treatment. Cancer 2016;122:2399–2407. © 2016 American Cancer Society.
Nested and interleaved direct sequence spread spectrum to enhance CDMA security and bit error rate performance
Authors: Falahati Abolfazl , Sanandaji Nader 
Published: May 2016

SummaryTwo new methods, the nested direct sequence spread spectrum and interleaved direct sequence spread spectrum, are introduced in view of the previously investigated spread spectrum methods. In the nested direct sequence method, the security is maintained, and the cross‐correlation between different codes resulting in multi‐user interference that corresponds to BER performance is improved, while in the interleaved direct sequence spread spectrum, the security is improved and BER performance is maintained. Both methods are analysed by mathematical relations as well as computer simulations and are compared with the existing methods. Copyright © 2016 John Wiley & Sons, Ltd.
Type 2 diabetes, insulin treatment and prognosis of breast cancer
Authors: Mu Lan , Zhu Nannan , Zhang Jiao , Xing Fang , Li Daiqing , Wang Xin 
Published: May 2016

AbstractBackgroundThe aim of our study was to investigate whether pre‐existing type 2 diabetes and insulin therapy have an impact on the prognosis of breast cancer patients.MethodsWe performed a retrospective analysis of 462 type 2 diabetic breast cancer patients and 1644 non‐diabetic breast cancer patients treated in our institute from January 2005 to August 2010. Patients were divided by diabetes status and insulin use. The clinicopathological characteristics and clinical outcomes of patients within 5 years following breast cancer diagnosed were analysed.ResultsDiabetic patients tended to have higher body mass index and higher histological grade tumours. Five‐year disease‐free survival and overall survival were reduced in diabetic patients (P < 0.001), and diabetes was an independent predictor for an increased risk of breast cancer relapse and death within 5 years (P < 0.001). Insulin treatment was associated with reduced 5‐year disease‐free survival and overall survival (P < 0.05); the risk of 5‐year relapse and breast cancer mortality in the insulin group increased compared to that of non‐insulin group after adjusting for age, tumour size, histological grade, oestrogen receptor, progesterone receptor, chemotherapy and hormone therapy (P < 0.05). After adjusting for age and other factors, the risk of breast cancer relapse was also increased in the insulin subgroup, while the risk of breast cancer mortality did not increase statistically.ConclusionsType 2 diabetes and insulin treatment might be independently associated with poorer prognosis of breast cancer. However, caution is needed when interpreting our results, and further investigations are needed. Copyright © 2016 John Wiley & Sons, Ltd.
Spectroelectrochemistry of EuCl3 in Four Molten Salt Eutectics; 3 LiCl−NaCl, 3 LiCl−2 KCl, LiCl−RbCl, and 3 LiCl−2 CsCl; at 873 K
Authors: Schroll Cynthia A. , Chatterjee Sayandev , Levitskaia Tatiana , Heineman William R. , Bryan Samuel A. 
Published: May 2016

AbstractKey electrochemical properties affecting pyroprocessing of nuclear fuel were examined in four eutectic melts using Eu3+/2+ as a representative probe. We report the electrochemical and spectroelectrochemical behavior of EuCl3 in four molten salt eutectics (3 LiCl−NaCl, 3 LiCl−2 KCl, LiCl−RbCl and 3 LiCl−2 CsCl) at 873 K. Cyclic voltammetry was used to determine the reduction potential for Eu3+/2+ and the applied potentials for spectroelectrochemistry. Single step chronoabsorptometry and thin‐layer spectroelectrochemistry were used to obtain the number of electrons transferred, reduction potentials and diffusion coefficients for Eu3+ in each eutectic melt. The reduction potentials determined by thin‐layer spectroelectrochemistry were essentially the same as those obtained using cyclic voltammetry. The diffusion coefficient for Eu3+ was the largest in the 3 LiCl−NaCl melt, showed a negative shift in the 3 LiCl−2 KCl melt, and was the smallest in the LiCl−RbCl and 3 LiCl−2 CsCl eutectic melts. The basic one‐electron reversible electron transfer for Eu3+/2+ was not affected by melt composition.
Characterizing supraglacial meltwater channel hydraulics on the Greenland Ice Sheet from in situ observations
Authors: Gleason Colin J. , Smith Laurence C. , Chu Vena W. , Legleiter Carl J. , Pitcher Lincoln H. , Overstreet Brandon T. , Rennermalm Asa K. , Forster Richard R. , Yang Kang 
Published: May 2016

AbstractSupraglacial rivers on the Greenland Ice Sheet (GrIS) transport large volumes of surface meltwater toward the ocean, yet have received relatively little direct research. This study presents field observations of channel width, depth, velocity, and water surface slope for nine supraglacial channels on the south‐western GrIS collected between July 23 and August 20, 2012. Field sites are located up to 74 km inland and span 494–1485 m elevation, and contain measured discharges larger than any previous in situ study: from 0.006 to 23.12 m3/s in channels 0.20 to 20.62 m wide. All channels were deeply incised with near vertical banks, and hydraulic geometry results indicate that supraglacial channels primarily accommodate greater discharges by increasing velocity. Smaller streams had steeper water surface slopes (0.74–8.83%) than typical in terrestrial settings, yielding correspondingly high velocities (0.40–2.60 m/s) and Froude numbers (0.45–3.11) with supercritical flow observed in 54% of measurements. Derived Manning's n values were larger and more variable than anticipated from channels of uniform substrate, ranging from 0.009 to 0.154 with a mean value of 0.035 ± 0.027 despite the absence of sediment, debris, or other roughness elements. Ubiquitous micro‐depressions in shallow sections of the channel bed may explain some of these roughness values. However, we find that other, unobserved sources of flow resistance likely contributed to these elevated Manning's n values: future work should explicitly consider additional sources of flow resistance beyond bed roughness in supraglacial channels. We conclude that hydraulic modeling for these channels must allow for both subcritical and supercritical flow, and most importantly must refrain from assuming that all ice‐substrate channels exhibit similar hydraulic behavior, especially for Froude numbers and Manning's n. Finally, this study highlights that further theoretical and empirical work on supraglacial channel hydraulics is necessary before broad scale understanding of ice sheet hydrology can be achieved. Copyright © 2016 John Wiley & Sons, Ltd.
Androgens and androgenic activity in broiler manure assessed by means of chemical analyses and in vitro bioassays
Authors: Valdehita Ana , Fernández-Cruz María-Luisa , González-Gullón María Isabel , Becerra-Neira Eduardo , Delgado María Mar , García-González Mari Cruz , Navas José María 
Published: May 2016

Abstract

The use of manure as an agricultural amendment is increasing the release of steroid hormones into the environment. Most research in this field has focused on estrogenic phenomena, with less attention paid to androgenic substances. The present study assessed androgenic activity in broiler manure using in vitro approaches based on cells stably transfected with androgen receptor. Leaching experiments were also performed to observe whether endocrine disruptors present in manure pass through a soil column and potentially reach groundwater. In parallel, an analytical chemistry method was used to determine the contribution of the most important natural androgens to androgenicity. Samplings were performed at 4 farms in 2 seasons. All but 2 samples showed androgen activity. In leakage experiments, however, no androgenic activity was detectable in leachates or in soils after leaching. According to the analytical results, androgenicity can be attributed mainly (but not completely) to androstenedione, and dihydrotestosterone. Similarly to the bioassays, chemical analysis did not reveal the presence of any androgen in leachates or soils. These results point to a rapid degradation of the substances responsible for androgenic activity in soils under the experimental conditions of the present study. However, the long-term effects associated with the constant and intensive application of manure to agricultural land require further attention. Environ Toxicol Chem 2017;36:1746–1754. © 2016 SETAC