bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mathematical Note
Authors: Lewis E.P. 
Published: February 2017

1842. On Note 1727 : Extension of Simeon's line (Gazette, XXVIII, p. 107). Mr. Gibbins might be interested in G. A. Srinivasan's Note 605 (Gazette, XI, p. 20, January, 1922). My pendant to this note on p. 87 of Vol. XI was unfortunately wrecked by a misprint; I give its substance in 1 below. For a figure, see Note 1727 (July, 1944).
In 2 and 3 I give a geometrical proof of the theorem about the join of the orthocentres.
Isospectral surfaces of small genus
Authors: Brooks Roberts , Tse Richard 
Published: February 2017

In this note, we will construct simple examples of isospectral surfaces. In what follows, we will use the term “surface” to mean a surface endowed with a Riemannian metric, while the term “Riemann surface” will be reserved for a surface endowed with a metric of constant curvature. We will show:
THEOREM 1. There exist pairs of surfaces S1 and S2 of genus 3, such that S1 and S2 are isospectral but not isometric.THEOREM 2. There exist pairs of Riemann surfaces S1 and S2 of genus 4 and 6, which are isospectral but not isometric.THEOREM 3. There exist unoriented surfaces S1 and S2 of Euler characteristic X(S1) = X(S2) = — 6 which are isospectral but not isometric.
Responses: Political Science, Democracy, and Authoritarianism
Authors: Colton Timothy J. , Hale Henry E. , March Luke , Robertson Graeme B. 
Published: February 2017

Stephen Kotkin is surely right that Russia cannot be understood fully through the lens of its elections and that it is conceptually risky for political scientists to treat U.S. democracy as its analytical point of departure. He also makes a good point that governance, institutional quality, and actual state performance need to be studied along with civil society and political parties.Fortunately, today's field of political science offers a wide range of works that agree. Without producing a long bibliography, we might mention Kathryn Stoner-Weiss's and Daniel Treisman's recent books on center-periphery relations and governance; Maria Popova's and Peter H. Solomon's research into the Russian judiciary; Lucan Way's study of the institutional underpinnings of authoritarianism; and lively debates on the state's management of the economy and its ability to provide social services.
Bracketing the Copan Dynasty: Late Preclassic and Early Postclassic Settlements at Copan, Honduras
Authors: Manahan T. Kam , Canuto Marcello A. 
Published: February 2017

AbstractArchaeological research within the Classic Maya center of Copan and in its surrounding rural regions has generated new data relating to the periods both preceding and following the center’s Classic period dynasty. Recent excavations at both Late Preclassic and Early Postclassic settlements have revealed more similarities between the inhabitants of these two “non-Classic” time periods than to the inhabitants of the intervening and better known Classic period. We explore this striking set of similarities in terms of settlement pattern, spatial organization, architecture, material culture, and ritual deposits and spaces. We suggest that the similarities between the Copan region’s Late Preclassic and Early Postclassic populations and their mutual differences with intervening Classic period peoples reflecta cultural connection between these two populations.
El Maíz (Zea Mays) en el Mundo Prehispánico de Chile Central
Authors: Falabella Fernanda , Planella M. Teresa , Tykot Robert H. 
Published: February 2017

ResumenLos restos de maíz en sitios arqueológicos de la región central de Chile son notablemente escasos. Para estudiar la introducción y adopción de este cultivo en las poblaciones prehispánicas se discutirán los resultados de análisis de isótopos estables de un conjunto de muestras humanas que abarcan desde las comunidades alfareras iniciales (300 a.C.–200 d.C.) hasta el contacto incaico (1450–1550 d.C.). Los isótopos estables de Carbono son sensibles para determinar la presencia y estimar abundancia de plantas C4, como el Zea mays, en dietas del pasado. Nuestros resultados permiten concluir que i) la adopción del maíz es posterior al 200 d.C., ii) su consumo se hace regular entre los grupos Llolleo, iii) su uso aumenta significativamente en los grupos Aconcagua con un consumo diferencial por género, y finalmente iv) la importancia del Zea mays decae inesperadamente durante el período de contacto incaico. En el Período Cerámico Temprano, los horticultores de maíz eran contemporáneos de y compartían el paisaje con cazadores recolectores y horticultores de quinoa, aún cuando cada grupo tenía sus propios hábitos dietarios. Esta evidencia muestra un nuevo acercamiento a los sistemas sociales pero también dejan abiertas algunas preguntas, especialmente en lo que se refiere a la presencia inicial del maíz en la región.
Un Aporte Crítico a Partir de “Comentarios Sobre Montículos de la Cuenca de la Laguna Merín: Tiempo, Espacio Y Sociedad”
Authors: Boksar Roberto Bracco , Puerto Laura del , Inda Hugo , García-Rodríguez Felipe 
Published: February 2017

ResumenSe abordan cuatro temas en la replica a Iriarte et. al (en este volumen): 1) Reconstrucciones paleoclimáticas, analizando la baja resolución del registro de Iriarte (2006), limitaciones del procedimiento de muestreo, sistema acuático elegido y dataciones. Se responde a las criticas sobre las inferencias paleoclimdticas realizadas, indicando que no consideran el total de las líneas de evidencia utilizadas ni la escala del registro y señalando la buena correspondencia con reconstrucciones propuestas para la región. 2) Evidencias de aldeas estructuradas en sitios con estructuras monticulares. Observamos las argumenlaciones esgrimidas para el sitio de Los Ajos, refiriendolas al contexto arqueológico de la región. Se responde a nuestra supuesta falta de observation de cambios culturales y ambientales, señalándose los problemas de las secuencias culturales propuestas. Se retoma el problema de la mecánica de construcción de los montículos como cuestión inicial a la horn de interpretar el registro y postular secuencias culturales. 3) Dataciones, desarrollando el concepto de fechados de baja resolutión y su pertinencia en la propuesta del Modelo de Crecimiento Continuo para las estructuras monticulares. 4) Por último se observa las implicaciones de la confusión entre la cuenca del Plata y la cuenca de la Laguna Merín.
CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis
Authors: Shin GiWon , Grimes Susan M. , Lee HoJoon , Lau Billy T. , Xia Li C. , Ji Hanlee P. 
Published: February 2017

AbstractMicrosatellites are multi-allelic and composed of short tandem repeats (STRs) with individual motifs composed of mononucleotides, dinucleotides or higher including hexamers. Next-generation sequencing approaches and other STR assays rely on a limited number of PCR amplicons, typically in the tens. Here, we demonstrate STR-Seq, a next-generation sequencing technology that analyses over 2,000 STRs in parallel, and provides the accurate genotyping of microsatellites. STR-Seq employsin vitroCRISPR–Cas9-targeted fragmentation to produce specific DNA molecules covering the complete microsatellite sequence. Amplification-free library preparation provides single molecule sequences without unique molecular barcodes. STR-selective primers enable massively parallel, targeted sequencing of large STR sets. Overall, STR-Seq has higher throughput, improved accuracy and provides a greater number of informative haplotypes compared with other microsatellite analysis approaches. With these new features, STR-Seq can identify a 0.1% minor genome fraction in a DNA mixture composed of different, unrelated samples.
Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer
Authors: Andretta Elena , Cartón-García Fernando , Martínez-Barriocanal Águeda , de Marcondes Priscila Guimarães , Jimenez-Flores Lizbeth M. , Macaya Irati , Bazzocco Sarah , Bilic Josipa , Rodrigues Paulo , Nieto Rocio , Landolfi Stefania , Ramon y Cajal Santiago , Schwartz Simo , Brown Arthur , Dopeso Higinio , Arango Diego 
Published: February 2017

AbstractEPH signaling deregulation has been shown to be important for colorectal carcinogenesis and genome-wide sequencing efforts have identified EPHA3 as one of the most frequently mutated genes in these tumors. However, the role of EPHA3 in colorectal cancer has not been thoroughly investigated. We show here that ectopic expression of wild type EPHA3 in colon cancer cells did not affect their growth, motility/invasion or metastatic potential in vivo. Moreover, overexpression of mutant EPHA3 or deletion of the endogenous mutant EPHA3 in colon cancer cells did not affect their growth or motility. EPHA3 inactivation in mice did not initiate the tumorigenic process in their intestine, and had no effects on tumor size/multiplicity after tumor initiation either genetically or pharmacologically. In addition, immunohistochemical analysis of EPHA3 tumor levels did not reveal associations with survival or clinicopathological features of colorectal cancer patients. In conclusion, we show that EPHA3 does not play a major role in colorectal tumorigenesis. These results significantly contribute to our understanding of the role of EPH signaling during colorectal carcinogenesis, and highlighting the need for detailed functional studies to confirm the relevance of putative cancer driver genes identified in sequencing efforts of the cancer genome.
A new perspective on studying burial environment before archaeological excavation: analyzing bacterial community distribution by high-throughput sequencing
Authors: Xu Jinjin , Wei Yanfei , Jia Hanqing , Xiao Lin , Gong Decai 
Published: February 2017

AbstractBurial conditions play a crucial role in archaeological heritage preservation. Especially, the microorganisms were considered as the leading causes which incurred degradation and vanishment of historic materials. In this article, we analyzed bacterial diversity and community structure from M1 of Wangshanqiao using 16 S rRNA gene amplicon sequencing. The results indicated that microbial communities in burial conditions were diverse among four different samples. The samples from the robber hole varied most obviously in community structure both in Alpha and Beta diversity. In addition, the dominant phylum in different samples wereProteobacteria, ActinobacteriaandBacteroidetes, respectively. Moreover, the study implied that historical materials preservation conditions had connections with bacterial community distribution. At the genus level,Acinetobactermight possess high ability in degrading organic culture heritage in burial conditions, whileBacteroideswere associated closely with favorable preservation conditions. This method contributes to fetch information which would never recover after excavation, and it will help to explore microbial degradation on precious organic culture heritage and further our understanding of archaeological burial environment. The study also indicates that robbery has a serious negative impact on burial remains.
Polarization conversion in cubic Raman crystals
Authors: McKay Aaron , Sabella Alexander , Mildren Richard P. 
Published: February 2017

AbstractNonlinear conversion of unpolarized beams to lower frequencies is generally inefficient in c(2) materials, as it is challenging to achieve phase-matching for input ordinary and extraordinary beams simultaneously in the normal dispersion regime. Here, we show that cubic Raman crystals having doubly and triply degenerate (E and F type) modes provide a method for efficient nonlinear frequency downconversion of an unpolarized beam and yield a linearly polarized output state. Using Mueller calculus, optimal crystal directions for such polarization conversion are determined. Using diamond, an example of an F-class Raman crystal, we have verified that such conversion is possible with near quantum-defect-limited slope efficiency and a linear polarization contrast of more than 23.9 dB.
Variation among conventional cultivars could be used as a criterion for environmental safety assessment of Bt rice on nontarget arthropods
Authors: Wang Fang , Dang Cong , Chang Xuefei , Tian Junce , Lu Zengbin , Chen Yang , Ye Gongyin 
Published: February 2017

AbstractThe current difficulty facing risk evaluations of Bacillus thuringiensis (Bt) crops on nontarget arthropods (NTAs) is the lack of criteria for determining what represents unacceptable risk. In this study, we investigated the biological parameters in the laboratory and field population abundance of Nilaparvata lugens (Hemiptera: Delphacidae) on two Bt rice lines and the non-Bt parent, together with 14 other conventional rice cultivars. Significant difference were found in nymphal duration and fecundity of N. lugens fed on Bt rice KMD2, as well as field population density on 12 October, compared with non-Bt parent. However, compared with the variation among conventional rice cultivars, the variation of each parameter between Bt rice and the non-Bt parent was much smaller, which can be easily seen from low-high bar graphs and also the coefficient of variation value (C.V). The variation among conventional cultivars is proposed to be used as a criterion for the safety assessment of Bt rice on NTAs, particularly when statistically significant differences in several parameters are found between Bt rice and its non-Bt parent. Coefficient of variation is suggested as a promising parameter for ecological risk judgement of IRGM rice on NTAs.
Accelerating Corrosion of Pure Magnesium Co-implanted with Titanium in Vivo
Authors: Hou Peng , Han Pei , Zhao Changli , Wu Hongliu , Ni Jiahua , Zhang Shaoxiang , Liu Jingyi , Zhang Yuanzhuang , Xu Haidong , Cheng Pengfei , Liu Shen , Zheng Yufeng , Zhang Xiaonong , Chai Yimin 
Published: February 2017

AbstractMagnesium is a type of reactive metal, and is susceptible to galvanic corrosion. In the present study, the impact of coexistence of Ti on the corrosion behavior of high purity Mg (HP Mg) was investigated both in vitro and in vivo. Increased corrosion rate of HP Mg was demonstrated when Mg and Ti discs were not in contact. The in vivo experiments further confirmed accelerating corrosion of HP Mg screws when they were co-implanted with Ti screws into Sprague-Dawley rats’ femur, spacing 5 and 10 mm. Micro CT scan and 3D reconstruction revealed severe corrosion morphology of HP Mg screws. The calculated volume loss was much higher for the HP Mg screw co-implanted with Ti screw as compared to that co-implanted with another Mg screw. Consequently, less new bone tissue ingrowth and lower pullout force were found in the former group. It is hypothesized that the abundant blood vessels on the periosteum act as wires to connect the Mg and Ti screws and form a galvanic-like cell, accelerating the corrosion of Mg. Therefore, a certain distance is critical to maintain the mechanical and biological property of Mg when it is co-implanted with Ti.
Sequestration of Ribosome during Protein Aggregate Formation: Contribution of ribosomal RNA
Authors: Pathak Bani K. , Mondal Surojit , Banerjee Senjuti , Ghosh Amar Nath , Barat Chandana 
Published: February 2017

AbstractAn understanding of the mechanisms underlying protein aggregation and cytotoxicity of the protein aggregates is crucial in the prevention of several diseases in humans. Ribosome, the cellular protein synthesis machine is capable of acting as a protein folding modulator. The peptidyltransferase center residing in the domain V of large ribosomal subunit 23S rRNA is the centre for the protein folding ability of the ribosome and is also the cellular target of several antiprion compounds. Our in vitro studies unexpectedly reveal that the partial unfolding or aggregation of lysozyme under reducing conditions in presence of the ribosome can induce aggregation of ribosomal components. Electrostatic interactions complemented by specific rRNA-protein interaction drive the ribosome-protein aggregation process. Under similar conditions the rRNA, especially the large subunit rRNA and in vitro transcribed RNA corresponding to domain V of 23S rRNA (bDV RNA) stimulates lysozyme aggregation leading to RNA-protein aggregate formation. Protein aggregation during the refolding of non-disulfide containing protein BCAII at high concentrations also induces ribosome aggregation. BCAII aggregation was also stimulated in presence of the large subunit rRNA. Our observations imply that the specific sequestration of the translation machine by aggregating proteins might contribute to their cytotoxicity.
Secreted frizzled-related protein 1 overexpression in gastric cancer: Relationship with radiological findings of dual-energy spectral CT and PET-CT
Authors: Lin Huimin , Yang Guoyuan , Ding Bei , Zhang Miao , Zhang Mingjun , Yan Fuhua , Qu Ying , Zhang Huan 
Published: February 2017

AbstractWe explored the role of secreted frizzled-related protein 1 (sFRP1) overexpression in gastric cancer and its relationship with radiological findings from dual-energy spectral CT(DEsCT) and positron emission tomography/computed tomography (PET/CT). We established mouse metastatic models using the SGC-7901/sFRP1 gastric cancer cell line. A control group was established using the SGC-7901/vector cell line. The models were then scanned with dual-energy spectral CT and PET-CT. Subsequent analysis, including immunohistochemistry and Transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL), was performed to confirm the role of sFRP1. Transwell chamber and angiogenesis assays were conducted to verify the effect of sFRP1 in vitro. We found that the control group showed negative radiological performance with successful implantation. Concurrently, the treated group showed visible lesions, a higher FDG uptake and increasing enhancement. The immunological and histological analysis confirmed the positive radiological performance with larger size, increasing proliferation, more microvessels and less apoptosis. The angiogenic up-regulation of sFRP1 overexpression were further verified with in vitro cell models. This preliminary study demonstrates that sFRP1 overexpression in gastric cancer cells leads to increased cell proliferation and angiogenesis, which may, in turn, contribute to positive PET/CT and CT performances.
Aza-heterocyclic Receptors for Direct Electron Transfer Hemoglobin Biosensor
Authors: Kumar Vinay , Kashyap D. M. Nikhila , Hebbar Suraj , Swetha R. , Prasad Sujay , Kamala T. , Srikanta S. S. , Krishnaswamy P. R. , Bhat Navakanta 
Published: February 2017

AbstractDirect Electron Transfer biosensors, facilitating direct communication between the biomolecule of interest and electrode surface, are preferable compared to enzymatic and mediator based sensors. Although hemoglobin (Hb) contains four redox active iron centres, direct detection is not possible due to inaccessibility of iron centres and formation of dimers, blocking electron transfer. Through the coordination of iron with aza-heterocyclic receptors - pyridine and imidazole - we report a cost effective, highly sensitive and simple electrochemical Hb sensor using cyclic voltammetry and chronoamperometry. The receptor can be either in the form of liquid micro-droplet mixed with blood or dry chemistry embedded in paper membrane on top of screen printed carbon electrodes. We demonstrate excellent linearity and robustness against interference using clinical samples. A truly point of care technology is demonstrated by integrating disposable test strips with handheld reader, enabling finger prick to result in less than a minute.
The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression
Authors: Gonzalez-Vasconcellos I. , Schneider R. , Anastasov N. , Alonso-Rodriguez S. , Sanli-Bonazzi B. , Fernández J. L. , Atkinson M. J. 
Published: February 2017

AbstractThe tumour suppressor gene (Rb1) is necessary for the maintenance of telomere integrity in osteoblastic cells. We now show that the compaction of telomeric chromatin and the appropriate histone modifications of telomeric DNA are both dependent upon Rb1-mediated transcription of the telomere-derived long non-coding RNA TERRA. Expression of TERRA was reduced in Rb1 haploinsufficient cells, and further decreased by shRNA-mediated reduction of residual Rb1 expression. Restoration of Rb1 levels through lentiviral transduction was sufficient to reestablish both transcription of TERRA and condensation of telomeric chromatin. The human chromosome 15q TERRA promoter contains predicted retinoblastoma control elements, and was able to confer Rb1-dependent transcription upon a promoterless reporter gene. Chromatin immunoprecipitation revealed preferential binding of phosphorylated over non-phosphorylated Rb1 at the TERRA promoter. As Rb1-deficient cells show increased genomic instability we suggest that this novel non-canonical action of Rb1 may contribute to the tumour suppressive actions of Rb1.
Operator Radiation and the Efficacy of Ceiling-Suspended Lead Screen Shielding during Coronary Angiography: An Anthropomorphic Phantom Study Using Real-Time Dosimeters
Authors: Jia Qianjun , Chen Ziman , Jiang Xianxian , Zhao Zhenjun , Huang Meiping , Li Jiahua , Zhuang Jian , Liu Xiaoqing , Hu Tianyu , Liang Wensheng 
Published: February 2017

AbstractOperator radiation and the radiation protection efficacy of a ceiling-suspended lead screen were assessed during coronary angiography (CA) in a catheterization laboratory. An anthropomorphic phantom was placed under the X-ray beam to simulate patient attenuation in eight CA projections. Using real-time dosimeters, radiation dose rates were measured on models mimicking a primary operator (PO) and an assistant. Subsequently, a ceiling-suspended lead screen was placed in three commonly used positions to compare the radiation protection efficacy. The radiation exposure to the PO was 2.3 to 227.9 (mean: 67.2 ± 49.0) μSv/min, with the left anterior oblique (LAO) 45°/cranial 25° and cranial 25° projections causing the highest and the lowest dose rates, respectively. The assistant experienced significantly less radiation overall (mean: 20.1 ± 19.6 μSv/min, P < 0.003), with the right anterior oblique (RAO) 30° and cranial 25° projections resulting in the highest and lowest exposure levels, respectively. Combined with table-side shielding, the ceiling-suspended lead screen reduced the radiation to the PO by 76.8%, 81.9% and 93.5% when placed close to the patient phantom, at the left side and close to the PO, respectively, and reduced the radiation to the assistant by 70.3%, 76.7% and 90.0%, respectively. When placed close to the PO, a ceiling-suspended lead screen provides substantial radiation protection during CA.
Room Temperature Characteristics of Polymer-Based Low Ice Adhesion Surfaces
Authors: He Zhiwei , Vågenes Elisabeth T. , Delabahan Chrisrosemarie , He Jianying , Zhang Zhiliang 
Published: February 2017

AbstractIce adhesion is mainly dictated by surface properties, and water wettability is frequently correlated with ice adhesion strength. However, these established correlations are limited to high ice adhesion and become invalid when the ice adhesion strength is low. Here we carried out an experimental study to explore the relationships between low ice adhesion strength and room temperature surface properties. A variety of room temperature properties of 22 polymer-based hydrophilic and hydrophobic samples consisting of both low and high ice adhesion surfaces were analysed. The properties investigated include water adhesion force, water wettability, roughness, elastic modulus and hardness. Our results show that low ice adhesion strength does not correlate well with water contact angle and its variants, surface roughness and hardness. Low elastic modulus does not guarantee low ice adhesion, however, surfaces with low ice adhesion always show low elastic modulus. Low ice adhesion (below 60 kPa) of tested surfaces may be determinative of small water adhesion force (from 180 to 270 μN). Therefore, measurement of water adhesion force may provide an effective strategy for screening anti-icing or icephobic surfaces, and surfaces within specific values of water adhesion force will possibly lead to a low ice adhesion.