bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered dNTP pools accelerate tumor formation in mice
Authors: Tran Phong , Mishra Pradeep , Williams Leonard G , Moskalenko Roman , Sharma Sushma , Nilsson Anna Karin , Watt Danielle L , Andersson Pernilla , Bergh Anders , Pursell Zachary F , Chabes Andrei 
Published: October 2024

Abstract
Alterations in deoxyribonucleoside triphosphate (dNTP) pools have been linked to increased mutation rates and genome instability in unicellular organisms and cell cultures. However, the role of dNTP pool changes in tumor development in mammals remains unclear. In this study, we present a mouse model with a point mutation at the allosteric specificity site of ribonucleotide reductase, RRM1-Y285A. This mutation reduced ribonucleotide reductase activity, impairing the synthesis of deoxyadenosine triphosphate (dATP) and deoxyguanosine triphosphate (dGTP). Heterozygous Rrm1+/Y285A mice exhibited distinct alterations in dNTP pools across various organs, shorter lifespans and earlier tumor onset compared with wild-type controls. Mutational spectrum analysis of tumors revealed two distinct signatures, one resembling a signature extracted from a human cancer harboring a mutation of the same amino acid residue in ribonucleotide reductase, RRM1Y285C. Our findings suggest that mutations in enzymes involved in dNTP metabolism can serve as drivers of cancer development.
RAD52-mediated repair of DNA double-stranded breaks at inactive centromeres leads to subsequent apoptotic cell death
Authors: Maruta Gen , Maeoka Hisanori , Tsunoda Toshiyuki , Akiyoshi Kozaburo , Takagi Satoshi , Shirasawa Senji , Ishikura Shuhei 
Published: October 2024

Abstract
Centromeres, where the kinetochore complex binds, are susceptible to damages including DNA double-stranded breaks (DSBs). Here, we report the functional significance and the temporally and spatially distinct regulation of centromeric DSB repair via the three pathways of non-homologous end joining (NHEJ), homologous recombination (HR) and single-strand annealing (SSA). The SSA factor RAD52 is most frequently recruited to centromeric DSB sites compared with the HR factor RAD51 and the NHEJ factor DNA ligase IV (LIG4), indicating that SSA plays predominant roles in centromeric DSB repair. Upon centromeric DSB induction, LIG4 is recruited to both active centromeres, where kinetochore complex binds, and inactive centromeres. In contrast, RAD51 and RAD52 are recruited only to inactive centromeres. These results indicate that DSBs at active centromeres are repaired through NHEJ, whereas the three pathways of NHEJ, HR and SSA are involved in DSB repair at inactive centromeres. Furthermore, siRNA-mediated depletion of either LIG4 or RAD51 promotes cell death after centromeric DSB induction, whereas RAD52 depletion inhibits it, suggesting that HR and NHEJ are required for appropriate centromeric DSB repair, whereas SSA-mediated centromeric DSB repair leads to subsequent cell death. Thus, SSA-mediated DSB repair at inactive centromeres may cause centromere dysfunction through error-prone repair.
PDCdb: the biological activity and pharmaceutical information of peptide–drug conjugate (PDC)
Authors: Sun Xiuna , Li Hanyang , Chen Zhen , Zhang Yang , Wei Zhangle , Xu Hangwei , Liao Yang , Jiang Wanghao , Ge Yichao , Zheng Lingyan , Li Teng , Wu Yuting , Luo Meiyin , Fang Luo , Dong Xiaowu , Xiao Mang , Han Lianyi , Jia Qingzhong , Zhu Feng 
Published: October 2024

Abstract
Peptide–drug conjugates (PDCs) have emerged as a promising class of targeted therapeutics with substantial pharmaceutical advantages and market potentials, which is a combination of a peptide (selective to the disease-relevant target), a linker (stable in circulation but cleavable at target site) and a cytotoxic/radioactive drug (efficacious/traceable for disease). Among existing PDCs, those based on radiopharmaceuticals (a.k.a. radioactive drugs) are valued due to their accurate imaging and targeted destruction of disease sites. It's demanded to accumulate the biological activity and pharmaceutical information of PDCs. Herein, a database PDCdb was thus constructed to systematically describe these valuable data. Particularly, biological activities for 2036 PDCs were retrieved from literatures, which resulted in 1684, 613 and 2753 activity data generated based on clinical trial, animal model and cell line, respectively. Furthermore, the pharmaceutical information for all 2036 PDCs was collected, which gave the diverse data of (a) ADME property, plasma half-life and administration approach of a PDC and (b) chemical modification, primary target, mode of action, conjugating feature of the constituent peptide/linker/drug. In sum, PDCdb systematically provided the biological activities and pharmaceutical information for the most comprehensive list of PDCs among the available databases, which was expected to attract broad interest from related communities and could be freely accessible at: https://idrblab.org/PDCdb/
Pediatric metastatic extracranial high-grade glioma: A case report and literature review
Authors: Ali Naba , Eaton Bree R , Fangusaro Jason R , Castellino Robert C , Velázquez Vega José E , Chern Joshua J , Schniederjan Matthew , Patil Prabhumallikarjun 
Published: October 2024

Abstract
We report a case of a 10-year-old male with a right frontal diffuse pediatric-type high-grade glioma (HGG), H3-wild-type (WT), and IDH-WT, diagnosed at the age of 9 years, who underwent gross total resection, 60 Gy focal proton radiation in 30 fractions to the resection cavity with concurrent temozolomide followed by maintenance chemotherapy with temozolomide and lomustine. One month after completion of maintenance chemotherapy, he developed subcutaneous swelling in the right temporal region and was treated with antibiotics for presumed lymphadenitis. Two months later, he developed a recurrent painless right parietal soft tissue mass that failed to respond to antibiotic therapy. This prompted evaluation by MRI which revealed new enhancing masses in the cerebellum and extracranial soft tissue mass in the right temporal region. He underwent gross total resection of both masses. Pathologic analysis confirmed both masses as recurrent HGG. Molecular markers, however, differed between the 2 sites of recurrence. He proceeded to complete hypofractionated proton therapy at sites of recurrence. Three months later, he was found to have tumor dissemination into the spine and brain for which he received proton therapy to the whole spine and brain. Due to the presence of CDK4 amplification at diagnosis and both sites of tumor recurrence, he then received palliative treatment with the CDK4/6 inhibitor, abemaciclib, for the final 5 months of his life. Since extracranial HGG is a rare presentation, with few cases reported in the pediatric population, we report this case and review previously published literature.
Strengthening Fungal Infection Diagnosis and Treatment: An In-depth Analysis of Capabilities in Honduras
Authors: Ortiz Bryan , Varela Diana , Fontecha Gustavo , Torres Karla , Cornely Oliver A , Salmanton-García Jon 
Published: October 2024

Abstract

Background
Invasive fungal infections (IFIs) are a major public health concern in low- and middle-income countries (LMICs) due to limited diagnostic and treatment resources, leading to high morbidity and mortality. Despite their significant global burden, IFIs are underrecognized and underdiagnosed in LMICs. This study evaluates the diagnostic and therapeutic capacities for managing IFI in Honduras, a country with unique health care challenges.

Methods
From March to December 2023, a comprehensive survey was conducted across multiple health care centers in Honduras. The survey, reviewed for content and clarity by local medical institutions, targeted medical microbiologists and clinicians to assess various aspects of fungal disease diagnosis and treatment. Data included the availability and use of diagnostic tools and antifungal therapies, identifying gaps and limitations in current practices.

Results
The survey revealed that Candida spp (97.4%) and Aspergillus spp (35.9%) were the most concerning pathogens. Although microscopy and culture methods were available in most institutions, their application in suspected IFI cases was inconsistent, and antifungal susceptibility testing was rarely performed. Advanced diagnostic techniques, such as antigen detection, were available in only a few institutions, while antibody detection and polymerase chain reaction testing were entirely absent. All hospitals had access to at least 1 triazole antifungal, typically fluconazole, but there was a notable scarcity of more potent antifungals, including amphotericin B formulations and echinocandins. The limited use of available diagnostic tools and the restricted availability of essential antifungals were identified as major barriers to effective IFI management.

Conclusions
This study highlights significant gaps in the diagnostic and therapeutic capabilities for managing IFI in Honduras. The underutilization of basic diagnostic tools, the inaccessibility of advanced testing methods, and the limited availability of essential antifungal medications underscore the urgent need for capacity-building initiatives, infrastructure improvements, and policy reforms. Addressing these deficiencies is critical for enhancing the management of IFI in Honduras, with broader implications for similar LMIC settings. These findings can inform targeted interventions and resource allocation to improve outcomes for patients with IFI.
A universal scaling relation incorporating the cusp-to-core transition of dark matter halos
Authors: Kaneda Yuka , Mori Masao , Otaki Koki 
Published: October 2024

Abstract
The dark matter halos associated with galaxies have hitherto established strong correlations within a range of observed parameters, known as scaling relations of dark matter halos. The origin of these scaling relations still contains significant ambiguities and requires comprehensive exploration for complete understanding. Utilising the correlation between the concentration and mass of dark matter halos inferred from cosmological N-body simulations based on the cold dark matter paradigm (c–M relation), we derive theoretical scaling relations among other physical quantities, such as the surface mass density, the maximum circular velocity, and the scale radius of the dark matter halo. By comparing theoretical and observed scaling relations at various mass scales, it is found that the scaling relations observed in dwarf galaxies and galaxies originate in the c–M relation of the dark matter halo. We predict that this theoretical scaling relation is also established in galaxy clusters. Moreover, we propose a novel theoretical scaling relation that incorporates the effects of the cusp-to-core transition, which is supposed to occur in cold dark matter halos. Our discussion concludes with the exploration of potential observational verification of the cusp-to-core transition process in dark matter halos.
Examining the effectiveness of a police developed cardio-pulmonary resuscitation virtual reality training program
Authors: Keningale Paige , Halford Eric , Taleb Hussain AlShaima , Condon Camie , Bullock Karen 
Published: October 2024

Abstract
In 2020 the Saif Bin Zayed Academy for Security & Policing Sciences in the United Arab Emirates created a Police Virtual Training Centre, known as the Abu Dhabi Police Virtual Training Centre. Since their inception they have internally developed more than 12 virtual reality simulations. This includes a Cardio-Pulmonary Resuscitation (CPR) Virtual Reality (VR) Training Program. Delivered to both police officers and civilian employees the CPR VR replicates a realistic cardiac arrest incident. The purpose of this study is to evaluate the effectiveness of this VR simulation. This is achieved by using a quantitative survey methodology to test the knowledge acquired from two groups who received CPR training using VR and a second who received it using traditional classroom tuition. Results are compared inferential statistics and identified no significant difference in test outcomes, indicating the VR simulation is safe. In addition, we surveyed both groups regarding their views and perspectives of the training environment and we used the presence scale for virtual reality to test the degree of immersion, environmental fidelity and for negative impacts. Correlational analysis identified a strong link between the immersive nature of VR, and levels of engagement and realism. Negative effects of discomfort and disorientation were strongly correlated with one another but were not linked to the level of immersion. The findings are discussed in the context of potentially using VR to supplement or replace existing police CPR training, and the wider considerations regarding developing VR within policing.
L’espace vécu and Its Perturbations in Schizophrenia: Systematic Review and Meta-analysis of Altered Body-Centric Metrics—Personal and Peripersonal Space
Authors: Raballo Andrea , Poletti Michele , Bevione Francesco , Lacidogna Maria Carla , Preti Antonio 
Published: October 2024

Abstract
Subtle distortions of the experience of lived space have long been associated with schizophrenia. Although a body-centric transformation of space is considered an essential component of anomalous subjective experience in schizophrenia, its impact on the 2 major body-centric spatial constructs, that is, personal space (PS) and peripersonal space (PPS), is still not clear. This systematic review and meta-analysis have been set up to: (1) summarize the evidence on putative extensional differences of PS and PPS in schizophrenia as compared with controls, and (2) evaluate the quality and the limitations of available studies on the topic. Four electronic literature databases (MEDLINE, EMBASE, PsychINFO, and CINAHL) were searched with the keywords “Personal space OR Interpersonal distance AND Schizophrenia,” “Peripersonal space AND Schizophrenia” from inception until December 31, 2023, resulting in 15 studies on PS and 5 studies on PPS included in this systematic review. The 12 studies on PS included in the meta-analysis revealed that individuals with a diagnosis of schizophrenia place a larger interpersonal distance from the stimuli than controls, with a moderate effect size in both the fixed-effect model (Hedges’ g = 0.558 [95% confidence interval, CI: 0.445–0.671]; z = 9.67; P &lt; 0.0001) and the random effects model (0.547 [0.294–0.799]; z = 4.77; P = 0.0006). The 5 studies included in the meta-analysis on PPS showed that individuals with a diagnosis of schizophrenia exhibit a narrower PPS than the controls at the fixed-effect (Hedges’ g = 1.043 [95%CI: .739–1.348]; z = 6.72; P &lt; .0001), but not at the random effects model (1.318 [−0.721 to 3.359]; z = 1.79; P = .147). Heterogeneity was substantial in both meta-analyses. Overall, the findings indicate that both body-centered space constructs (PS and PPS) are affected in schizophrenia, with an enlargement PS and a reduction PPS, thereby supporting the distinction of these constructs. These modifications cohere with the subjective transformation of the lived space (aka espace vécu) reported in classical psychopathology and may be promising, neurodevelopmentally grounded, biomarkers of vulnerability to schizophrenia and its spectrum conditions.
“Infantgram?” recruitment of infants to a clinical sleep study via social media
Authors: O’Connor Cathal , O’Leary Hannah , Murray Deirdre , Boylan Geraldine B 
Published: October 2024

Abstract

Study Objectives
This study aimed to outline the strategy and outcomes of a study team in recruiting participants for an infant sleep study via social media during the COVID-19 pandemic, to assess the feasibility of recruitment via social media, and to quantitatively and qualitatively explore parental satisfaction and perceptions of recruitment via social media.

Methods
The assessing sleep in infants with early-onset atopic dermatitis by longitudinal evaluation (SPINDLE) study recruited infants with and without atopic dermatitis for a longitudinal study assessing sleep. Infants were recruited via social media and their parents were interviewed to explore their experience of recruitment via social media.

Results
In total, 57 controls and 33 cases were recruited. Of the 45 controls recruited via social media, 43 (95.6%) were recruited via Instagram and 2 (4.4%) were recruited via Twitter. Of the seven cases recruited via social media, 6 (85.7%) were recruited via Facebook (via sharing of Instagram posts by third parties on Facebook) and 1 (14.3%) was recruited via Instagram. All (100%, n = 28) mothers recruited via social media who completed the full study were satisfied with this approach to recruitment. Specific reasons why mothers reported engaging following exposure to the social media posts included the benefit of additional health checks for their baby, the benefit to scientific advancement, and the opportunity for a stimulating outing following the COVID-19 lockdowns.

Conclusions
Our experience highlights parents’ acceptance of recruitment via social media, the optimization of time and financial resources, and the benefit of using internet-based recruitment during a pandemic.
The Right to Move a Private Member’s Bill in Uganda
Authors: Mujuzi Jamil Ddamulira 
Published: October 2024

Abstract
Under Article 94(4)(b) of the Constitution, ‘a member of Parliament has the right to move a private member’s bill [PMB]’. Under Article 94(4)(d), ‘the office of the Attorney-General shall afford the member moving the private member’s bill professional assistance in the drafting of the bill’. Article 93 prohibits Parliament from proceeding with a PMB that, inter alia, imposes a charge on the Consolidated Fund. Rule 121(3) of the Rules of Parliament provides ‘[t]he Clerk to Parliament shall, where necessary, afford the Member moving the’ PMB ‘professional assistance in the drafting of the Bill’. Under Rule 122(1), a PMB has to be introduced by way of motion which has to be carried for it to be passed. The right under Article 94(4)(b) is not one of the fundamental rights. The author relies on the drafting history of Articles 93 and 94 to demonstrate the nature of this right. He relies on the Hansard (2016–2024) to highlight how this right has been protected. He argues that Rules 121(3) and 122(2) are unconstitutional and that the Constitutional Court’s interpretation of Article 93(a)(ii) in one of its decisions is contrary to the drafting history of that provision.
A twin transition in regulatory toxicology: moving toward Chemicals 2.0 and phasing out animal testing
Authors: Worth Andrew P , Berggren Elisabet 
Published: October 2024

Abstract
The European regulatory framework on chemicals is at a crossroads. There are calls for the framework to be more effective, by better protecting people and the environment. There is also room for it to be more efficient and cost-effective, by harmonizing assessment practices across sectors and avoiding the need for unnecessary testing. At the same time, there is a political commitment to phase out animal testing in chemical safety assessments. In this commentary, we argue that these needs are not at odds with each other. On the contrary, the European Commission’s roadmap to phase out animal testing could also be the transition pathway to a more efficient, effective, and sustainable regulatory ecosystem. Central to our proposal is a framework based on biological reasoning in which biological questions can be answered by a choice of methods, with non-animal methods progressively becoming the only choice. Within this framework, a tiered approach to testing and assessment allows for greater efficiency and effectiveness, while also introducing considerations of proportionality and cost-effectiveness. Testing strategies, and their component methods, should be developed in tandem and judged in terms of their outcomes, and the protection levels they inform, rather than their ability to predict the outputs of animal tests.
Initial stomatal conductance increases photosynthetic induction of trees leaves more from sunlit than from shaded environments: a meta-analysis
Authors: Kang Huixing , Yu Yuan , Ke Xinran , Tomimatsu Hajime , Xiong Dongliang , Santiago Louis , Han Qingmin , Kardiman Reki , Tang Yanhong 
Published: October 2024

Abstract
It has long been held that tree species/leaves from shaded environments show faster rate of photosynthetic induction than species/leaves from sunlit environments, but the evidence so far is conflicting and the underlying mechanisms are still under debate. To address the debate, we compiled a dataset for 87 tree species and compared the initial increasing slope during the first 2-min induction (SA) and stomatal and biochemical characteristics between sun and shade species from the same study, and those between sun and shade leaves within the same species. In 77% of between-species comparisons, the species with high steady-state photosynthetic rate in the high light (Af) exhibited a larger SA than the species with low Af. In 67% within-species comparisons, the sun leaves exhibited a larger SA than the shade leaves. However, in only a few instances did the sun species/leaves more rapidly achieve 50% of full induction, with an even smaller SA, than the shade species/leaves. At both the species and leaf level, SA increased with increasing initial stomatal conductance before induction (gsi). Despite exhibiting reduced intrinsic water-use efficiency in low light, a large SA proportionally enhances photosynthetic carbon gain during the first 2-min induction in the sun species and leaves. Thus, in terms of the increase in absolute rate of photosynthesis, tree species/leaves from sunlit environments display faster photosynthetic induction responses than those from shaded environments. Our results call for re-consideration of contrasting photosynthetic strategies in photosynthetic adaption/acclimation to dynamic light environments across species.