bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gonorrhea in Indonesia: High Prevalence of Asymptomatic Urogenital Gonorrhea but No Circulating Extended Spectrum Cephalosporins-Resistant Neisseria gonorrhoeae Strains in Jakarta, Yogyakarta, and Denpasar, Indonesia
Authors: Hananta I Putu Yuda , van Dam Alje P , Bruisten Sylvia Maria , Schim van der Loeff Maarten Franciscus , Soebono Hardyanto , de Vries Henry John Christiaan 
Published: September 2016

Background
Little is known about the epidemiology of asymptomatic urogenital gonorrhea and antimicrobial susceptibility of Neisseria gonorrhoeae (NG) strains circulating in Indonesia. We studied these issues in 3 large Indonesian cities.

Methods
In 2014, participants were recruited from sexually transmitted infection clinics and through outreach in Jakarta, Yogyakarta, and Denpasar. Neisseria gonorrhoeae detection in genital specimens was performed with NG-qPCR at the Public Health Service in Amsterdam. Antimicrobial susceptibility was investigated using the Etest.

Results
Among 992 participants, 781 were asymptomatic and included in the risk factors analysis: 439 (56.2%) men, 258 (33.0%) women, and 84 (10.8%) transwomen. They differed significantly in age and were mostly men who have sex with men (35.2%) and female sex workers (29.3%).
Overall, 175 (22.4%) asymptomatic participants had a positive NG-qPCR result. Factors positively associated with asymptomatic urogenital gonorrhea were being recruited through outreach (vs clinic-based), inconsistent condom use, and being divorced/widowed (vs single).
Among 79 urogenital cultured isolates derived from 27 symptomatic and 52 asymptomatic participants, all isolates were susceptible to ceftriaxone and cefixime, and 98.7% of isolates were susceptible to azithromycin. In contrast, resistance to doxycycline (98.7%) and ciprofloxacin (97.4%) was common.

Conclusions
Prevalence of asymptomatic urogenital gonorrhea among Indonesian key populations is very high. Little to no resistance against extended spectrum cephalosporins and azithromycin was observed. However, almost all isolates were resistant to doxycycline and ciprofloxacin. Strengthening outreach sexually transmitted infections services, composing guidelines to screen asymptomatic individuals, and implementing periodical antimicrobial resistance surveillance are recommended.
Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques
Authors: Mashiko Takanobu , Wu Szu-Hsien , Feng Jingwei , Kanayama Koji , Kinoshita Kaori , Sunaga Ataru , Narushima Mitsunaga , Yoshimura Kotaro 
Published: September 2016

Background:
Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.

Methods:
Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emulsified by repeated transfer between two syringes through a small-hole connecter and then separated by mesh filtration into two portions: residual tissue of emulsified fat and filtrated fluid of emulsified fat. The four products were examined for cellular components.

Results:
Histologic and electron microscopic analyses revealed that squeezed fat and residual tissue of emulsified fat contained broken adipocytes and fragmented capillaries. Compared with centrifuged fat, the squeezed fat and residual fat products exhibited increased specific gravity and increased numbers of adipose-derived stem/stromal cells and endothelial cells per volume, suggesting successful cell/tissue condensation in both squeezed fat and residual tissue of emulsified fat. Although cell number and viability in the stromal vascular fraction were well maintained in both squeezed fat and residual fat, stromal vascular fraction culture assay showed that adipose-derived stromal cells were relatively damaged in residual tissue of emulsified fat but not in squeezed fat. By contrast, no adipose-derived stromal cells were cultured from filtrated fluid of emulsified fat.

Conclusions:
The authors’ results demonstrated that mechanical micronization is easily conducted as a minimal manipulation procedure, which can condense the tissue by selectively removing adipocytes without damaging key components, such as adipose-derived stromal cells and endothelial cells. Depending on the extent of adipocyte removal, the product may be a useful therapeutic tool for efficient tissue volumization or therapeutic revitalization/fertilization.

CLINICAL QUESTION/LEVEL OF EVIDENCE:
Therapeutic, V.
Depression and Risk of Sudden Cardiac Death and Arrhythmias: A Meta-Analysis
Authors: Shi Shaobo , Liu Tao , Liang Jinjun , Hu Dan , Yang Bo 
Published: September 2016

ABSTRACT

Objective
Depression is an independent risk factor for cardiac events and mortality in individuals with or without cardiovascular disease (CVD), although the underlying mechanisms involved in sudden cardiac death (SCD) and arrhythmias remain unclear. This meta-analysis aimed to assess the relationship between depression and risk of SCD and arrhythmias.

Methods
We systematically searched MEDLINE, Elsevier, and PsycINFO databases for articles (January 1990 to June 2015) describing the correlation of depression (“depressive symptoms,” “depression,” or “depressive disorder”) with SCD or arrhythmias (ventricular tachycardia/ventricular fibrillation [VT/VF], or atrial fibrillation [AF]). Data were meta-analyzed with random-effects models.

Results
A total of 17 studies met the inclusion criteria: 4 of SCD (n = 83,659), 8 of VT/VF (n = 4,048), and 5 of AF (n = 31,247). The total sample consisted of 8,533 individuals with and 110,421 individuals without previous CVD. Depression was associated with increased risk of SCD (hazard risk [HR], 1.62; 95% confidence interval [CI], 1.37–1.92; p < .001), VT/VF (HR, 1.47; 95% CI, 1.23–1.76; p < .001) and AF recurrence (HR, 1.88; 95% CI, 1.54–2.30; p < .001). There was no significant association, however, between depression and risk of new-onset AF (HR, 0.96; 95% CI, 0.87–1.04; p = .311).

Conclusions
Depression (clinical depression and depressive symptoms) is associated with increased risk of SCD, VT/VF, and AF recurrence. These findings suggest that arrhythmias play an important role in the association between depression and increased mortality in individuals with or without CVD. Systematic evaluation and treatment of depression may contribute to the prevention of lethal cardiac events in the general population and in high-risk individuals with CVD.
Evidence that lower socioeconomic position accentuates genetic susceptibility to obesity
Authors: Tyrrell Jessica , Wood Andrew R , Ames Ryan M , Yaghootkar Hanieh , Beaumont Robin N. , Jones Samuel E. , Tuke Marcus A , Ruth Katherine S. , Freathy Rachel M , Smith George Davey , Joost Stéphane , Guessous Idris , Murray Anna , Strachan David P. , Kutalik Zoltán , Weedon Michael N , Frayling Timothy M 
Published: September 2016

AbstractSusceptibility to obesity in today’s environment has a strong genetic component. Lower socioeconomic position (SEP) is associated with a higher risk of obesity but it is not known if it accentuates genetic susceptibility to obesity. We aimed to use up to 120,000 individuals from the UK Biobank study to test the hypothesis that measures of socioeconomic position accentuate genetic susceptibility to obesity. We used the Townsend deprivation index (TDI) as the main measure of socioeconomic position, and a 69-variant genetic risk score (GRS) as a measure of genetic susceptibility to obesity. We also tested the hypothesis that interactions between BMI genetics and socioeconomic position would result in evidence of interaction with individual measures of the obesogenic environment and behaviours that correlate strongly with socioeconomic position, even if they have no obesogenic role. These measures included self-reported TV watching, diet and physical activity, and an objective measure of activity derived from accelerometers. We performed several negative control tests, including a simulated environment correlated with BMI but not TDI, and sun protection use. We found evidence of gene-environment interactions with TDI (Pinteraction=3×10−10) such that, within the group of 50% living in the most relatively deprived situations, carrying 10 additional BMI-raising alleles was associated with approximately 3.8 kg extra weight in someone 1.73m tall. In contrast, within the group of 50% living in the least deprivation, carrying 10 additional BMI-raising alleles was associated with approximately 2.9 kg extra weight. We also observed evidence of interaction between sun protection use and BMI genetics, suggesting that residual confounding may result in evidence of non-causal interactions. Our findings provide evidence that relative social deprivation best captures aspects of the obesogenic environment that accentuate the genetic predisposition to obesity in the UK.
Optokinetic nystagmus reflects perceptual directions in the onset binocular rivalry in Parkinson’s disease
Authors: Fujiwara Mana , Ding Catherine , Kaunitz Lisandro , Stout Julie C , Thyagarajan Dominic , Tsuchiya Naotsugu 
Published: September 2016

AbstractOptokinetic nystagmus (OKN), the reflexive eye movements evoked by a moving field, has recently gained interest among researchers as a useful tool to assess conscious perception. When conscious perception and stimulus are dissociated, such as in binocular rivalry —when dissimilar images are simultaneously presented to each eye and perception alternates between the two images over time — OKN correlates with perception rather than with the physical direction of the moving field. While this relationship is well established in healthy subjects it is yet unclear whether it also generalizes to clinical populations, for example, patients with Parkinson’s disease. Parkinson’s disease is a motor disorder, causing tremor, slow movements and rigidity. It may also be associated with oculomotor deficits, such as impaired saccades and smooth pursuit eye movements. Here, we employed short-duration, onset binocular rivalry (2 s trial of stimulus presentation followed by 1 s inter-trial interval) with moving grating stimuli to assess OKN in Parkinson’s disease patients (N=39) and controls (N=29) of a similar age. Each trial was either non-rivalrous (same stimuli presented to both eyes) or rivalrous, as in binocular rivalry. We analyzed OKN to discriminate direction of stimulus and perception on a trial-by-trial basis. OKN reflected conscious perceptions in both groups. Treatment with anti-Parkinson drugs and deep brain stimulation improved motor ability of patients assessed by a standard scale of Parkinson’s disease, but did not impact on OKN. Furthermore, OKN-based measures were robust and their latencies were shorter than manual button-based measures in all subjects, regardless of stimulus condition. Our findings suggest that OKN can be used as an indicator of conscious perception in binocular rivalry even in Parkinson’s disease patients in whom impaired manual dexterity may render button-press reports less reliable.
Cotranslational assembly imposes evolutionary constraints on homomeric proteins
Authors: Natan Eviatar , Endoh Tamaki , Haim-Vilmovsky Liora , Chalancon Guilhem , Flock Tilman , Hopper Jonathan TS. , Kintses Bálint , Daruka Lejla , Fekete Gergely , Pál Csaba , Papp Balázs , Horvath Peter , Marsh Joseph A. , Elcock Adrian H. , Babu M Madan , Robinson Carol V. , Sugimoto Naoki , Teichmann Sarah A. 
Published: September 2016

AbstractThere is increasing evidence that some proteins fold during translation,i.e.cotranslationally, which implies that partial protein function, including interactions with other molecules, could potentially be unleashed early on during translation. Although little is known about cotranslational assembly mechanisms, for homomeric protein complexes, translation by the ribosome, folding and assembly, should be well-coordinated to avoid misassembly in the context of polysomes. We analysed 3D structures of homomers and identified a statistically significant trend conserved across evolution that supports this hypothesis: namely that homomeric contacts tend to be localized towards the C-terminus rather than N-terminus of homomeric polypeptide chains. To probe this in more detail, we expressed a GFP-based library of 611 homomericE. coligenes, and analyzing their folding and assemblyin vivo. Consistent with our hypothesis, interface residues tend to be located near the N-terminus in cotranslationally aggregating homomers. In order to dissect the mechanisms of folding and assembly under controlled conditions, we engineered a protein library with three variable components: (i) the position and type homomerization domain, (ii) the reporter domain and (iii) the linker length that connects the two. By analyzing the misassembly rates of these engineered constructsin vivo,in vitroandin silico, we confirmed our hypothesis that C-terminal homomerization is favorable to N-terminal homomerization. More generally, these results provide a set of spatiotemporal constraints within polypeptide chains that favor efficient assembly, with implications for protein evolution and design.
Hand2 Inhibits Kidney Specification While Promoting Vein Formation Within the Posterior Mesoderm
Authors: Perens Elliot A. , Garavito-Aguilar Zayra V. , Guio-Vega Gina P. , Peña Karen T. , Schindler Yocheved L. , Yelon Deborah 
Published: September 2016

AbstractProper organogenesis depends upon defining the precise dimensions of organ progenitor territories. Kidney progenitors originate within the intermediate mesoderm (IM), but the pathways that set the boundaries of the IM are poorly understood. Here, we show that the bHLH transcription factor Hand2 limits the size of the embryonic kidneyby restricting IM dimensions. The IM is expanded in zebrafishhand2mutants and is diminished whenhand2is overexpressed. Within the posterior mesoderm,hand2is expressed laterally adjacent to the IM. Venous progenitors arise between these two territories, andhand2promotes venous development while inhibiting IM formation at this interface. Furthermore,hand2and the co-expressed zinc-finger transcription factorosr1have functionally antagonistic influences on kidney development. Together, our data suggest thathand2functions in opposition toosr1to balance the formation of kidney and vein progenitors by regulating cell fate decisions at the lateral boundary of the IM.IMPACT STATEMENTThe Hand2 transcription factor regulates the dimensions of the kidney by controlling cell fate decisions at the interface between organ fields.
Information seeking and use in the context of minimalist lifestyles
Authors: Pollak Angela 
Published: September 2016

Purpose
The purpose of this paper is to describe information seeking and use (ISU) within the context of minimalist lifestyles and connect characteristics of living with less to theories of information poverty and resilience.

Design/methodology/approach
Naturalistic methods of inquiry describe minimalist lifestyles in a remote, rural context through semi-structured interviews with 24 adults. Environmental scanning and visual methods extended data collection retrospectively and longitudinally to span almost 118 years of community history. Qualitative thematic coding and analysis proceeded inductively and reflexively.

Findings
Living minimally in this environment results in adaptive strategies that compensate for lack of resources in general, and information resources specifically. Positive psycho-social attitudes such as optimism, creativity, curiosity, resourcefulness, and self-sufficiency continue to be important factors in developing resilience in information seeking practices.

Research limitations/implications
Information poverty is usually defined relatively, and often in relation to formal, macro-level environments. Focussing attention on informal, local level ISU reveals alternate varieties of knowledge, ways of knowing and characteristics that create information resilience in the face of sometimes profound deficits.

Practical implications
Highlights of positive aspects to ISU in this remote, rural context will be of interest to researchers and practitioners serving rural library systems.

Originality/value
This study provides an historical and contemporary glimpse into the ISU patterns of a previously unexamined population and context, those who live minimalist lifestyles in a remote and rural location.
Illusions of a “Bond”: tagging cultural products across online platforms
Authors: Desrochers Nadine , Laplante Audrey , Martin Kim , Quan-Haase Anabel , Spiteri Louise 
Published: September 2016

PurposeMost studies pertaining to social tagging focus on one platform or platform type, thus limiting the scope of their findings. The purpose of this paper is to explore social tagging practices across four platforms in relation to cultural products associated with the bookCasino Royale, by Ian Fleming.Design/methodology/approachA layered and nested case study approach was used to analyse data from four online platforms: Goodreads, Last.fm, WordPress, and public library social discovery platforms. The top-level case study focuses on the bookCasino Royale, by Ian Fleming and its derivative products. The analysis of tagging practices in each of the four online platforms is nested within the top-level case study.Casino Royalewas conceptualized as a cultural product (the book), its derived products (e.g. movies, theme songs), as well as a keyword in blogs. A qualitative, inductive, and context-specific approach was chosen to identify commonalities in tagging practices across platforms whilst taking into account the uniqueness of each platform.FindingsThe four platforms comprise different communities of users, each platform with its own cultural norms and tagging practices. Traditional access points in the library catalogues focused on the subject, location, and fictitious characters of the book. User-generated content across the four platforms emphasized historical events and periods related to the book, and highlighted more subjective access points, such as recommendations, tone, mood, reaction, and reading experience. Revealing shifts occur in the tags between the original book and its cultural derivatives: Goodreads and library catalogues focus almost exclusively on the book, while Last.fm and WordPress make in addition cross-references to a wider range of different cultural products, including books, movies, and music. The analyses also yield apparent similarities in certain platforms, such as recurring terms, phrasing and composite or multifaceted tags, as well as a strong presence of genre-related terms for the book and music.Originality/valueThe layered and nested case study approach presents a more comprehensive theoretical viewpoint and methodological framework by which to explore the study of user-generated metadata pertaining to a range of related cultural products across a variety of online platforms.
Linking physicians’ medical practice information needs, resources and barriers to job satisfaction
Authors: Kostagiolas Petros , Gorezis Panagiotis , Martzoukou Konstantina , Deligeorgis Dimitrios , Niakas Dimitris 
Published: September 2016

PurposeMedical doctors seek information in order to satisfy their demanding everyday work practices and professional development endeavours. Information seeking is a continuous goal-related process that has impact on how they perceive and experience their job. The purpose of this paper is to explore the association of doctors’ awareness of medical practice information needs (MPIN), their frequency of using online information resources and the barriers they encountered during information seeking with their overall job satisfaction. More specifically, the research examined the mediating role of these information seeking related variables (information needs, online scholarly resources and information barriers) on doctors’ overall job satisfaction.Design/methodology/approachThe study employed a questionnaire survey of 138 medical doctors working within the context of a University Hospital in Greece. The survey took place between February and March 2014. To test the hypotheses the authors conducted regression analysis, hierarchical moderated analysis and bootstrapping using SPSS macro developed by Preacher and colleagues.FindingsThe statistical analysis found that higher awareness of MPIN had an indirect effect on doctors’ overall job satisfaction when they used online information scholar resources. In addition, this indirect effect was contingent on information-related barriers.Originality/valueThis study provides evidence for supporting the pivotal role of doctors’ information seeking preferences in fostering job satisfaction. This is an understudied research area that deserves a unique focus particularly with the constantly expanding medical information space that has impact on doctors’ medical practices and professional activities.
The role of agency in historians’ experiences of serendipity in physical and digital information environments
Authors: Martin Kim , Quan-Haase Anabel 
Published: September 2016

Purpose
The purpose of this paper is to investigate the changing research practices of historians, and to contrast their experiences of serendipity in physical and digital information environments.

Design/methodology/approach
In total, 20 historians in Southwestern Ontario participated in semi-structured, in-depth interviews. The interviews were transcribed and analyzed employing grounded theory. The analytical approach included memoing, the constant comparative method, and three phases of coding.

Findings
Four main themes were identified: agency, the importance of the physical library experience, digital information environments, and novel heuristic forms of serendipity. The authors found that scholars frequently used active verbs to describe their experience with serendipity. This suggests that agency is more involved in the experience than previous conceptualizations of serendipity have suggested, and led us to coin the term “incidental serendipity.” Other key findings include the need for digital tools to incorporate the context surrounding primary sources, and also to provide an organizational context much like what is encountered by patrons in library stacks.

Originality/value
The increased emphasis on digital materials should not come at the expense of the physical information environment, where historians often encounter serendipitous finds. A fine balance and a greater integration between digital and physical resources is needed in order to support scholars’ continued ability to make connections between materials. By showing the active role that historians take in their serendipitous encounters, this paper suggests that historical training is critical for eliciting incidental serendipitous encounters. The authors propose a novel approach, one that examines verbs in serendipity accounts.