bluuebunny/crossref_metadata_2025_split huggingface dataset:
The long-term association of OCD and depression and its moderators: A four-year follow up study in a large clinical sample
Authors: Tibi L. , van Oppen P. , van Balkom A.J.L.M. , Eikelenboom M. , Rickelt J. , Schruers K.R.J. , Anholt G.E. 
Published: April 2017

AbstractBackground:Depression is the most common comorbidity in obsessive-compulsive disorder (OCD). However, the mechanisms of depressive comorbidity in OCD are poorly understood. We assessed the directionality and moderators of the OCD-depression association over time in a large, prospective clinical sample of OCD patients.Methods:Data were drawn from 382 OCD patients participating at the Netherlands Obsessive-Compulsive Disorder Association (NOCDA) study. Cross-lagged, structural equation modeling analyses were used to assess the temporal association between OCD and depressive symptoms. Assessments were conducted at baseline, two-year and four-year follow up. Cognitive and interpersonal moderators of the prospective association between OCD and depressive symptoms were tested.Results:Cross-lagged analyses demonstrated that OCD predicts depressive symptoms at two-year follow up and not vice a versa. This relationship disappeared at four-year follow up. Secure attachment style moderated the prospective association between OCD and depression.Conclusions:Depressive comorbidity in OCD might constitute a functional consequence of the incapacitating OCD symptoms. Both OCD and depression symptoms demonstrated strong stability effects between two-year and four-year follow up, which may explain the lack of association between them in that period. Among OCD patients, secure attachment represents a buffer against future depressive symptoms.
Design and Fabrication of Anisotropic Conductive Film for Application of Probe Card
Authors: Hsu W. H. , Chou M. C. , Tsai H. Y. 
Published: April 2017

AbstractPrior to integrated circuit (IC) packaging, die performance must be verified using probe cards to screen for defective products. With the decrease in IC line width, the dimensions of the pads used for performance verification and the spacing between adjacent pads have also decreased. However, when the pad pitch is reduced to less than 30 μm, commonly used probe cards will face manufacturing problems in miniaturization. To resolve probe card manufacturing problems caused by the miniaturization of IC components, the use of an anisotropic conductive film (ACF) in probe cards was proposed in this study. Theoretical calculations and experimental testing of this probe structure were conducted to demonstrate the feasibility of this concept.In theoretical calculations, composite material and buckling theory were utilized to evaluate the buckling behavior of the ACF. In experimental testing, photolithography and electroplating techniques were used to control the line width and spacing intervals of the micron-scale metal wires in the ACF. After the ACF was fabricated, the mechanical properties of the ACF during wafer testing were assessed. Theoretical analyses and experimental testing verified that ACFs can potentially be applied to the performance verification of IC products. In the ACF structure, multiple probes came into contact with each pad. Therefore, ACFs can potentially be applied to the performance verification of IC components with pad diameters of less than 20 μm. The results of this study directly benefit the miniaturization of ICs.
Norovirus epidemiology in South African children <5 years hospitalised for diarrhoeal illness between 2009 and 2013
Authors: PAGE N. A. , GROOME M. J. , NADAN S. , NETSHIKWETA R. , KEDDY K. H. , POONSAMY B. , MOYES J. , WALAZA S. , KAHN K. , MADHI S. A. , TAYLOR M. B. , MANS J. , COHEN C. 
Published: April 2017

SUMMARYPublic health interest in norovirus (NoV) has increased in recent years following improved diagnostics, global burden estimates and the development of NoV vaccine candidates. This study aimed to describe the detection rate, clinical characteristics and environmental features associated with NoV detection in hospitalized children <5 years with diarrhoea in South Africa (SA). Between 2009 and 2013, prospective diarrhoeal surveillance was conducted at four sites in SA. Stool specimens were collected and screened for NoVs and other enteric pathogens using molecular and serological assays. Epidemiological and clinical data were compared in patients with or without detection of NoV. The study detected NoV in 15% (452/3103) of hospitalized children <5 years with diarrhoea with the majority of disease in children <2 years (92%; 417/452). NoV-positive children were more likely to present with diarrhoea and vomiting (odds ratio (OR) 1·3; 95% confidence interval (CI) 1·1–1·7; P = 0·011) with none-to-mild dehydration (adjusted OR 0·5; 95% CI 0·3–0·7) compared with NoV-negative children. Amongst children testing NoV positive, HIV-infected children were more likely to have prolonged hospitalization and increased mortality compared with HIV-uninfected children. Continued surveillance will be important to consider the epidemic trends and estimate the burden and risk of NoV infection in SA.
Light-assisted delithiation of lithium iron phosphate nanocrystals towards photo-rechargeable lithium ion batteries
Authors: Paolella Andrea , Faure Cyril , Bertoni Giovanni , Marras Sergio , Guerfi Abdelbast , Darwiche Ali , Hovington Pierre , Commarieu Basile , Wang Zhuoran , Prato Mirko , Colombo Massimo , Monaco Simone , Zhu Wen , Feng Zimin , Vijh Ashok , George Chandramohan , Demopoulos George P. , Armand Michel , Zaghib Karim 
Published: April 2017

AbstractRecently, intensive efforts are dedicated to convert and store the solar energy in a single device. Herein, dye-synthesized solar cell technology is combined with lithium-ion materials to investigate light-assisted battery charging. In particular we report the direct photo-oxidation of lithium iron phosphate nanocrystals in the presence of a dye as a hybrid photo-cathode in a two-electrode system, with lithium metal as anode and lithium hexafluorophosphate in carbonate-based electrolyte; a configuration corresponding to lithium ion battery charging. Dye-sensitization generates electron–hole pairs with the holes aiding the delithiation of lithium iron phosphate at the cathode and electrons utilized in the formation of a solid electrolyte interface at the anode via oxygen reduction. Lithium iron phosphate acts effectively as a reversible redox agent for the regeneration of the dye. Our findings provide possibilities in advancing the design principles for photo-rechargeable lithium ion batteries.
Structural insights into POT1-TPP1 interaction and POT1 C-terminal mutations in human cancer
Authors: Chen Cong , Gu Peili , Wu Jian , Chen Xianyun , Niu Shuangshuang , Sun Hong , Wu Lijie , Li Na , Peng Junhui , Shi Shaohua , Fan Cuiying , Huang Min , Wong Catherine C. L. , Gong Qingguo , Kumar-Sinha Chandan , Zhang Rongguang , Pusztai Lajos , Rai Rekha , Chang Sandy , Lei Ming 
Published: April 2017

AbstractMammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends and regulates telomerase-mediated telomere extension. However, how POT1 interacts with TPP1 remains unknown. Here we present the crystal structure of the C-terminal portion of human POT1 (POT1C) complexed with the POT1-binding motif of TPP1. The structure shows that POT1C contains two domains, a third OB fold and a Holliday junction resolvase-like domain. Both domains are essential for binding to TPP1. Notably, unlike the heart-shaped structure of ciliated protozoan Oxytricha nova TEBPα–β complex, POT1–TPP1 adopts an elongated V-shaped conformation. In addition, we identify several missense mutations in human cancers that disrupt the POT1C–TPP1 interaction, resulting in POT1 instability. POT1C mutants that bind TPP1 localize to telomeres but fail to repress a DNA damage response and inappropriate repair by A-NHEJ. Our results reveal that POT1 C terminus is essential to prevent initiation of genome instability permissive for tumorigenesis.
Proximate effects of temperature versus evolved intrinsic constraints for embryonic development times among temperate and tropical songbirds
Authors: Ton Riccardo , Martin Thomas E. 
Published: April 2017

AbstractThe relative importance of intrinsic constraints imposed by evolved physiological trade-offs versus the proximate effects of temperature for interspecific variation in embryonic development time remains unclear. Understanding this distinction is important because slow development due to evolved trade-offs can yield phenotypic benefits, whereas slow development from low temperature can yield costs. We experimentally increased embryonic temperature in free-living tropical and north temperate songbird species to test these alternatives. Warmer temperatures consistently shortened development time without costs to embryo mass or metabolism. However, proximate effects of temperature played an increasingly stronger role than intrinsic constraints for development time among species with colder natural incubation temperatures. Long development times of tropical birds have been thought to primarily reflect evolved physiological trade-offs that facilitate their greater longevity. In contrast, our results indicate a much stronger role of temperature in embryonic development time than currently thought.
Global gene expression profiling of healthy human brain and its application in studying neurological disorders
Authors: Negi Simarjeet K. , Guda Chittibabu 
Published: April 2017

AbstractBrain function is governed by precise regulation of gene expression across its anatomically distinct structures; however, the expression patterns of genes across hundreds of brain structures are not clearly understood. Here, we describe a gene expression model, which is representative of the healthy human brain transcriptome by using data from the Allen Brain Atlas. Our in-depth gene expression profiling revealed that 84% of genes are expressed in at least one of the 190 brain structures studied. Hierarchical clustering based on gene expression profiles delineated brain regions into structurally tiered spatial groups and we observed striking enrichment for region-specific processes. Further, weighted co-expression network analysis identified 19 robust modules of highly correlated genes enriched with functional associations for neurogenesis, dopamine signaling, immune regulation and behavior. Also, structural distribution maps of major neurotransmission systems in the brain were generated. Finally, we developed a supervised classification model, which achieved 84% and 81% accuracies for predicting autism- and Parkinson’s-implicated genes, respectively, using our expression model as a baseline. This study represents the first use of global gene expression profiling from healthy human brain to develop a disease gene prediction model and this generic methodology can be applied to study any neurological disorder.
Therapeutic targeting using tumor specific peptides inhibits long non-coding RNA HOTAIR activity in ovarian and breast cancer
Authors: Özeş Ali R. , Wang Yinu , Zong Xingyue , Fang Fang , Pilrose Jay , Nephew Kenneth P. 
Published: April 2017

AbstractLong non-coding RNAs (lncRNAs) play key roles in human diseases, including cancer. Functional studies of the lncRNA HOTAIR (HOX transcript antisense RNA) provide compelling evidence for therapeutic targeting of HOTAIR in cancer, but targeting lncRNAs in vivo has proven to be difficult. In the current study, we describe a peptide nucleic acids (PNA)-based approach to block the ability of HOTAIR to interact with EZH2 and subsequently inhibit HOTAIR-EZH2 activity and resensitize resistant ovarian tumors to platinum. Treatment of HOTAIR-overexpressing ovarian and breast cancer cell lines with PNAs decreased invasion and increased chemotherapy sensitivity. Furthermore, the mechanism of action correlated with reduced nuclear factor-kappaB (NF-κB) activation and decreased expression of NF-κB target genes matrix metalloprotease 9 and interleukin 6. To deliver the anti-lncRNA to the acidic (pH approximately 6) tumor microenvironment, PNAs were conjugated to pH-low insertion peptide (pHLIP). Treatment of mice harboring platinum-resistant ovarian tumor xenografts with pHLIP-PNA constructs suppressed HOTAIR activity, reduced tumor formation and improved survival. This first report on pHLIP-PNA lncRNA targeting solid tumors in vivo suggests a novel cancer therapeutic approach.
Wideband, wide-angle coding phase gradient metasurfaces based on Pancharatnam-Berry phase
Authors: Zheng Qiqi , Li Yongfeng , Zhang Jieqiu , Ma Hua , Wang Jiafu , Pang Yongqiang , Han Yajuan , Sui Sai , Shen Yang , Chen Hongya , Qu Shaobo 
Published: April 2017

AbstractA new concept of the coding phase gradient metasurface (CPGM) is proposed, which is constructed using the phase gradient metasurface as the coding elements. Different from the previous coding metasurface (CM), both the coding sequences and gradient phases in the coding elements are designed to manipulate the electromagnetic (EM) wave for the CPGMs, and thus the manipulation will be more flexible. As an example, wide-band, wide-angle CPGMs with zero and non-zero phase gradient based on Pancharatnam-Berry (PB) phase are achieved using the co-polarization reflection unit cells under circularly polarized (CP) wave incidence. Both theoretically calculated and numerically simulated scattering patterns of the designed CPGMs demonstrate the expected manipulations. Additionally, two kinds of random CPGMs with different phase gradients are designed for radar cross section (RCS) reduction, and the measured RCS reveals a good accordance with the simulation.
Capturing RNA Folding Free Energy with Coarse-Grained Molecular Dynamics Simulations
Authors: Bell David R. , Cheng Sara Y. , Salazar Heber , Ren Pengyu 
Published: April 2017

AbstractWe introduce a coarse-grained RNA model for molecular dynamics simulations, RACER (RnA CoarsE-gRained). RACER achieves accurate native structure prediction for a number of RNAs (average RMSD of 2.93 Å) and the sequence-specific variation of free energy is in excellent agreement with experimentally measured stabilities (R2 = 0.93). Using RACER, we identified hydrogen-bonding (or base pairing), base stacking, and electrostatic interactions as essential driving forces for RNA folding. Also, we found that separating pairing vs. stacking interactions allowed RACER to distinguish folded vs. unfolded states. In RACER, base pairing and stacking interactions each provide an approximate stability of 3–4 kcal/mol for an A-form helix. RACER was developed based on PDB structural statistics and experimental thermodynamic data. In contrast with previous work, RACER implements a novel effective vdW potential energy function, which led us to re-parameterize hydrogen bond and electrostatic potential energy functions. Further, RACER is validated and optimized using a simulated annealing protocol to generate potential energy vs. RMSD landscapes. Finally, RACER is tested using extensive equilibrium pulling simulations (0.86 ms total) on eleven RNA sequences (hairpins and duplexes).
Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision
Authors: Gandier Julie-Anne , Langelaan David N. , Won Amy , O’Donnell Kylie , Grondin Julie L. , Spencer Holly L. , Wong Philip , Tillier Elisabeth , Yip Christopher , Smith Steven P. , Master Emma R. 
Published: April 2017

AbstractClass I hydrophobins are functional amyloids secreted by fungi. They self-assemble into organized films at interfaces producing structures that include cellular adhesion points and hydrophobic coatings. Here, we present the first structure and solution properties of a unique Class I protein sequence of Basidiomycota origin: the Schizophyllum commune hydrophobin SC16 (hyd1). While the core β-barrel structure and disulphide bridging characteristic of the hydrophobin family are conserved, its surface properties and secondary structure elements are reminiscent of both Class I and II hydrophobins. Sequence analyses of hydrophobins from 215 fungal species suggest this structure is largely applicable to a high-identity Basidiomycota Class I subdivision (IB). To validate this prediction, structural analysis of a comparatively distinct Class IB sequence from a different fungal order, namely the Phanerochaete carnosa PcaHyd1, indicates secondary structure properties similar to that of SC16. Together, these results form an experimental basis for a high-identity Class I subdivision and contribute to our understanding of functional amyloid formation.
Knockdown of toll-like receptor 4 signaling pathways ameliorate bone graft rejection in a mouse model of allograft transplantation
Authors: Hsieh Jeng-Long , Shen Po-Chuan , Wu Po-Ting , Jou I-Ming , Wu Chao-Liang , Shiau Ai-Li , Wang Chrong-Reen , Chong Hao-Earn , Chuang Shu-Han , Peng Jia-Shiou , Chen Shih-Yao 
Published: April 2017

AbstractNon-union occurring in structural bone grafting is a major problem in allograft transplantation because of impaired interaction between the host and graft tissue. Activated toll-like receptor (TLR) induces inflammatory cytokines and chemokines and triggers cell-mediated immune responses. The TLR-mediated signal pathway is important for mediating allograft rejection. We evaluated the effects of local knockdown of the TLR4 signaling pathway in a mouse segmental femoral graft model. Allografts were coated with freeze-dried lentiviral vectors that encoded TLR4 and myeloid differentiation primary response gene 88 (MyD88) short-hairpin RNA (shRNA), which were individually transplanted into the mice. They were assessed morphologically, radiographically, and histologically for tissue remodeling. Union occurred in autografted but not in allografted mice at the graft and host junctions after 4 weeks. TLR4 and MyD88 expression was up-regulated in allografted mice. TLR4 and MyD88 shRNAs inhibited TLR4 and MyD88 expression, which led to better union in the grafted sites. More regulatory T-cells in the draining lymph nodes suggested inflammation suppression. Local inhibition of TLR4 and MyD88 might reduce immune responses and ameliorate allograft rejection.
Long noncoding RNA LINC00305 promotes inflammation by activating the AHRR-NF-κB pathway in human monocytes
Authors: Zhang Dan-Dan , Wang Wen-Tian , Xiong Jian , Xie Xue-Min , Cui Shen-Shen , Zhao Zhi-Guo , Li Mulin Jun , Zhang Zhu-Qin , Hao De-Long , Zhao Xiang , Li Yong-Jun , Wang Junwen , Chen Hou-Zao , Lv Xiang , Liu De-Pei 
Published: April 2017

AbstractAccumulating data from genome-wide association studies (GWAS) have provided a collection of novel candidate genes associated with complex diseases, such as atherosclerosis. We identified an atherosclerosis-associated single-nucleotide polymorphism (SNP) located in the intron of the long noncoding RNA (lncRNA) LINC00305 by searching the GWAS database. Although the function of LINC00305 is unknown, we found that LINC00305 expression is enriched in atherosclerotic plaques and monocytes. Overexpression of LINC00305 promoted the expression of inflammation-associated genes in THP-1 cells and reduced the expression of contractile markers in co-cultured human aortic smooth muscle cells (HASMCs). We showed that overexpression of LINC00305 activated nuclear factor-kappa beta (NF-κB) and that inhibition of NF-κB abolished LINC00305-mediated activation of cytokine expression. Mechanistically, LINC00305 interacted with lipocalin-1 interacting membrane receptor (LIMR), enhanced the interaction of LIMR and aryl-hydrocarbon receptor repressor (AHRR), and promoted protein expression as well as nuclear localization of AHRR. Moreover, LINC00305 activated NF-κB exclusively in the presence of LIMR and AHRR. In light of these findings, we propose that LINC00305 promotes monocyte inflammation by facilitating LIMR and AHRR cooperation and the AHRR activation, which eventually activates NF-κB, thereby inducing HASMC phenotype switching.
EpCAM Intracellular Domain Promotes Porcine Cell Reprogramming by Upregulation of Pluripotent Gene Expression via Beta-catenin Signaling
Authors: Yu Tong , Ma Yangyang , Wang Huayan 
Published: April 2017

AbstractPrevious study showed that expression of epithelial cell adhesion molecule (EpCAM) was significantly upregulated in porcine induced pluripotent stem cells (piPSCs). However, the regulatory mechanism and the downstream target genes of EpCAM were not well investigated. In this study, we found that EpCAM was undetectable in fibroblasts, but highly expressed in piPSCs. Promoter of EpCAM was upregulated by zygotic activated factors LIN28, and ESRRB, but repressed by maternal factors OCT4 and SOX2. Knocking down EpCAM by shRNA significantly reduced the pluripotent gene expression. Conversely, overexpression of EpCAM significantly increased the number of alkaline phosphatase positive colonies and elevated the expression of endogenous pluripotent genes. As a key surface-to-nucleus factor, EpCAM releases its intercellular domain (EpICD) by a two-step proteolytic processing sequentially. Blocking the proteolytic processing by inhibitors TAPI-1 and DAPT could reduce the intracellular level of EpICD and lower expressions of OCT4, SOX2, LIN28, and ESRRB. We noticed that increasing intracellular EpICD only was unable to improve activity of EpCAM targeted genes, but by blocking GSK-3 signaling and stabilizing beta-catenin signaling, EpICD could then significantly stimulate the promoter activity. These results showed that EpCAM intracellular domain required beta-catenin signaling to enhance porcine cell reprogramming.
Longitudinal Impacts of Print-Focused Read-Alouds for Children With Language Impairment
Authors: Justice Laura M. , Logan Jessica , Kaderavek Joan N. 
Published: April 2017

Purpose
Preschoolers with language impairment (LI) are prime candidates for early-literacy interventions, given their susceptibility for future reading difficulties. To date, most studies of early-literacy interventions for this population has assessed short-term impacts, with limited attention to whether initial effects are sustained over time. This study was designed to evaluate longitudinal impacts of print-focused read-alouds implemented by early childhood special education teachers for a clinic sample of children with LI.

Method
Assessment data available for 172 children with LI were analyzed to examine their print knowledge 1-year postintervention. Measures examined children's alphabet knowledge, print concepts, and name-writing skills, which were used to derive a print-knowledge composite.

Results

Results of hierarchical linear models examining children's print knowledge at 1-year postintervention showed that the effect size (
d
= 0.20) favoring the treatment group was similar to that observed one year prior (
d
= 0.21) at the end of intervention, suggesting that results did not fade over time. Results also showed that children with LI and comorbid low nonverbal cognition benefited the most from the intervention delivered 1 year earlier.

Conclusion
The maintenance of short-term effects to 1-year postintervention supports the value of early childhood teachers using print-focused read-alouds to improve the early-literacy skills of children with LI in their classrooms.