bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conservation of alternative splicing in sodium channels reveals evolutionary focus on release from inactivation and structural insights into gating
Authors: Liavas A. , Lignani G. , Schorge S. 
Published: June 2017

Key points
Sodium channels are critical for supporting fast action potentials in neurons; even mutations which cause small changes in sodium channel activity can have devastating consequences for the function of the nervous system.
Alternative splicing also changes the activity of sodium channels, and while it is highly conserved, it is not known whether the functional role of this splicing is also conserved.
Our data reveal that splicing has a highly conserved impact on the availability of sodium channels during trains of rapid stimulations, and suggest that in one mammalian channel, Nav1.1 encoded by SCN1A, the increased availability of one splice variant is detrimental.
A model reproducing the effects of splicing on channel behaviour suggests that the voltage sensor in the first domain is a rate limiting step for release of the inactivation domain, and highlights the functional specialization of channel domains.
AbstractVoltage‐gated sodium channels are critical for neuronal activity, and highly intolerant to variation. Even mutations that cause subtle changes in the activity these channels are sufficient to cause devastating inherited neurological diseases, such as epilepsy and pain. However, these channels do vary in healthy tissue. Alternative splicing modifies sodium channels, but the functional relevance and adaptive significance of this splicing remain poorly understood. Here we use a conserved alternate exon encoding part of the first domain of sodium channels to compare how splicing modifies different channels, and to ask whether the functional consequences of this splicing have been preserved in different genes. Although the splicing event is highly conserved, one splice variant has been selectively removed from Nav1.1 in multiple mammalian species, suggesting that the functional variation in Nav1.1 is less well tolerated. We show for three human channels (Nav1.1, Nav1.2 and Nav1.7) that splicing modifies the return from inactivated to deactivated states, and the differences between splice variants are occluded by antiepileptic drugs that bind to and stabilize inactivated states. A model based on structural data can replicate these changes, and indicates that splicing may exploit a distinct role of the first domain to change channel availability, and that the first domain of all three sodium channels plays a role in determining the rate at which the inactivation domain dissociates. Taken together, our data suggest that the stability of inactivated states is under tight evolutionary control, but that in Nav1.1 faster recovery from inactivation is associated with negative selection in mammals.
First Complete
Squash leaf curl China virus
Genomic Segment DNA-A Sequence from East Timor
Authors: Maina Solomon , Edwards Owain R. , de Almeida Luis , Ximenes Abel , Jones Roger A. C. 
Published: June 2017

ABSTRACT

We present here the first complete
Squash leaf curl China virus
(SLCCV) genomic segment DNA-A sequence from East Timor. It was isolated from a pumpkin plant. When compared with 15 complete SLCCV DNA-A genome sequences from other world regions, it most resembled the Malaysian isolate MC1 sequence.
Complete Genome Sequence of Acinetobacter calcoaceticus CA16, a Bacterium Capable of Degrading Diesel and Lignin
Authors: Ho Margaret T. , Weselowski Brian , Yuan Ze-Chun 
Published: June 2017

ABSTRACT

We report here the complete assembled genome sequence of
Acinetobacter calcoaceticus
CA16, which is capable of utilizing diesel and lignin as a sole carbon source. CA16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. The assembled sequences will help elucidate potential metabolic pathways and mechanisms responsible for CA16’s hydrocarbon degradation ability.
Draft Genome Sequence of Methylocapsa palsarum NE2
T
, an Obligate Methanotroph from Subarctic Soil
Authors: Miroshnikov Kirill K. , Didriksen Alena , Naumoff Daniil G. , Huntemann Marcel , Clum Alicia , Pillay Manoj , Palaniappan Krishnaveni , Varghese Neha , Mikhailova Natalia , Mukherjee Supratim , Reddy T. B. K. , Daum Chris , Shapiro Nicole , Ivanova Natalia , Kyrpides Nikos , Woyke Tanja , Dedysh Svetlana N. , Svenning Mette M. 
Published: June 2017

ABSTRACT

Methylocapsa palsarum
NE2
T
is an aerobic, mildly acidophilic, obligate methanotroph. Similar to other
Methylocapsa
species, it possesses only a particulate methane monooxygenase and is capable of atmospheric nitrogen fixation. The genome sequence of this typical inhabitant of subarctic wetlands and soils also contains genes indicative of aerobic anoxygenic photosynthesis.
Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
Authors: Crovadore Julien , Asaff Torres Ali , Rodríguez Heredia Raúl , Cochard Bastien , Chablais Romain , Lefort François 
Published: June 2017

ABSTRACT
We report here the metagenomes of soils samples from a perennial cropping system of asparagus that was treated with two biostimulants. Two treatments were compared to an untreated control. Control soils samples were taken at the beginning and at end of the experiment.
Draft Genome Sequence of
Sphingomonas
sp. Strain Sph1(2015), Isolated from a Fouled Membrane Filter Used to Produce Drinking Water
Authors: de Vries Hendrik J. , Marshall Ian P. G. , Schreiber Lars , Plugge Caroline M. 
Published: June 2017

ABSTRACT

We report here the high-quality draft genome sequence of
Sphingomonas
sp. strain Sph1(2015), isolated from a fouled reverse osmosis membrane used for the production of high-quality drinking water. The draft sequence provides insights into the
modus operandi
of this strain to form biofilms on membrane surfaces. This knowledge offers tools to develop novel antifouling strategies.
Complete Genome Sequences of Two Human Oral Microbiome Commensals: Streptococcus salivarius ATCC 25975 and
S. salivarius
ATCC 27945
Authors: Butler Robert R. , Soomer-James Jahna T. A. , Frenette Michel , Pombert Jean-François 
Published: June 2017

ABSTRACT

Streptococcus salivarius
strains are significant contributors to the human oral microbiome. Some possess unique fimbriae that give them the ability to coaggregate and colonize particular oral structures. We present here the complete genomes of
Streptococcus salivarius
Lancefield K
−
/K
+
strains ATCC 25975 and ATCC 27945, which can and cannot, respectively, produce fimbriae.
Complete Genome Sequences of the Endophytic
Streptomyces
sp. Strains LUP30 and LUP47B, Isolated from Lucerne Plants
Authors: Franco Christopher M. M. , Adetutu Eric M. , Le Hoang X. , Ballard Ross A. , Araujo Ricardo , Tobe Shanan S. , Paul Bobby , Mallya Sandeep , Satyamoorthy Kapaetu 
Published: June 2017

ABSTRACT

The complete genome sequences of two endophytic
Streptomyces
sp. strains, LUP30 and LUP47B, were analyzed. These strains were isolated from surface-sterilized roots of lucerne plants from South Australia and were found to promote the growth of the rhizobial partner
in vitro
and significantly increased nodulation and nitrogen fixation in lucerne plants.
Genome Sequence of the Saprophytic Ascomycete
Epicoccum
nigrum
Strain ICMP 19927, Isolated from New Zealand
Authors: Fokin Mikhail , Fleetwood Damien , Weir Bevan S. , Villas-Boas Silas 
Published: June 2017

ABSTRACT

Epicoccum nigrum
is a common mitosporic fungus of the Didymellaceae (Ascomycota) family known for the production of numerous secondary metabolites. Here, we present the 34.7-Mbp draft genome sequence of strain ICMP 19927 assembled from a range of short-insert and long-insert Illumina libraries.
Cost-effectiveness of HPV-based cervical cancer screening in the public health system in Nicaragua
Authors: Campos Nicole G , Mvundura Mercy , Jeronimo Jose , Holme Francesca , Vodicka Elisabeth , Kim Jane J 
Published: June 2017

ObjectivesTo evaluate the cost-effectiveness of human papillomavirus (HPV) DNA testing (versus Papanicolaou (Pap)-based screening) for cervical cancer screening in Nicaragua.DesignA previously developed Monte Carlo simulation model of the natural history of HPV infection and cervical cancer was calibrated to epidemiological data from Nicaragua. Cost data inputs were derived using a micro-costing approach in Carazo, Chontales and Chinandega departments; test performance data were from a demonstration project in Masaya department.SettingNicaragua’s public health sector facilities.ParticipantsWomen aged 30–59 years.InterventionsScreening strategies included (1) Pap testing every 3 years, with referral to colposcopy for women with an atypical squamous cells of undetermined significance or worse result (‘Pap’); (2) HPV testing every 5 years, with referral to cryotherapy for HPV-positive eligible women (HPV cryotherapy or ‘HPV-Cryo’); (3) HPV testing every 5 years, with referral to triage with visual inspection with acetic acid (VIA) for HPV-positive women (‘HPV-VIA’); and (4) HPV testing every 5 years, with referral to Pap testing for HPV-positive women (‘HPV-Pap’).Outcome measuresReduction in lifetime risk of cancer and incremental cost-effectiveness ratios (ICER; 2015 US$ per year of life saved (YLS)).ResultsHPV-based screening strategies were more effective than Pap testing. HPV-Cryo was the least costly and most effective strategy, reducing lifetime cancer risk by 29.5% and outperforming HPV-VIA, HPV-Pap and Pap only, which reduced cancer risk by 19.4%, 12.2% and 10.8%, respectively. With an ICER of US$320/YLS, HPV-Cryo every 5 years would be very cost-effective using a threshold based on Nicaragua’s per capita gross domestic product of US$2090. Findings were robust across sensitivity analyses on test performance, coverage, compliance and cost parameters.ConclusionsHPV testing is very cost-effective compared with Pap testing in Nicaragua, due to higher test sensitivity and the relatively lower number of visits required. Increasing compliance with recommended follow-up will further improve the health benefits and value for public health dollars.
A cross-sectional study of the prevalence, awareness, treatment and control of hypertension in Shenzhen, China
Authors: Li Haitao , Yan Xinfeng , Deng Xiaoting , Yang Lizhen , Zhao Shaojuan , Zou Jianfeng , Luo Yi , Cao Sijing 
Published: June 2017

ObjectivesAn understanding of the awareness, treatment and control of hypertension is helpful to guide decision-making regarding interventions to reduce the risk for diseases with hypertension as a key risk factor. This study aimed to estimate the prevalence, awareness, treatment, and control of hypertension in Shenzhen, China.MethodsA cross-sectional study was conducted. We employed multistage cluster random sampling methods to select participants. A survey involving face-to-face interviews was conducted to collect the data. A total of 1676 participants finished the survey and formed the final analysis.ResultsWe found that the prevalence of hypertension was 17.6%. The rates of hypertension awareness, treatment and control were 48.8%, 51.4% and 43.2% respectively. Only 6.8% hypertensives were found to be managed by community health centres. Compared with the female participants, the males were found to have higher rates of prevalence (19.7% vs 15.7%; OR 1.47, 95% CI 1.10 to 1.97) and awareness (42.9% vs 38.1%; OR 2.35, 95% CI 1.28 to 4.33), but lower rates of medication treatments (20.5% vs 30.2%; OR 0.83, 95% CI 0.05 to 0.92) and control (12.8% vs 23.0%; OR 0.45, 95% CI 0.21 to 0.96). Migrants were more likely to be aware of hypertension (32.5% vs 44.3%; OR 0.57, 95% CI 0.30 to 0.90), reduce their salt intake (8.8% vs 18.1%; OR 0.40, 95% CI 0.15 to 0.94), and undertake regular monitoring of hypertension (1.3% vs 11.0%; OR 0.14, 95% CI 0.02 to 0.92) when compared with the locals.ConclusionsOur study finds that hypertension is an important public health burden in Shenzhen. It implies that strategies need to be developed to improve effective primary care management of hypertension. It also suggests a need to develop gender and household register tailored strategies for the prevention, detection, treatment and control of hypertension.
Peripherally InSerted CEntral catheter dressing and securement in patients with cancer: the PISCES trial. Protocol for a 2x2 factorial, superiority randomised controlled trial
Authors: Rickard Claire M , Marsh Nicole M , Webster Joan , Gavin Nicole C , Chan Raymond J , McCarthy Alexandra L , Mollee Peter , Ullman Amanda J , Kleidon Tricia , Chopra Vineet , Zhang Li , McGrail Matthew R , Larsen Emily , Choudhury Md Abu , Keogh Samantha , Alexandrou Evan , McMillan David J , Mervin Merehau Cindy , Paterson David L , Cooke Marie , Ray-Barruel Gillian , Castillo Maria Isabel , Hallahan Andrew , Corley Amanda , Geoffrey Playford E 
Published: June 2017

IntroductionAround 30% of peripherally inserted central catheters (PICCs) fail from vascular, infectious or mechanical complications. Patients with cancer are at highest risk, and this increases morbidity, mortality and costs. Effective PICC dressing and securement may prevent PICC failure; however, no large randomised controlled trial (RCT) has compared alternative approaches. We designed this RCT to assess the clinical and cost-effectiveness of dressing and securements to prevent PICC failure.Methods and analysisPragmatic, multicentre, 2×2 factorial, superiority RCT of (1) dressings (chlorhexidine gluconate disc (CHG) vs no disc) and (2) securements (integrated securement dressing (ISD) vs securement device (SED)). A qualitative evaluation using a knowledge translation framework is included. Recruitment of 1240 patients will occur over 3 years with allocation concealment until randomisation by a centralised service. For the dressing hypothesis, we hypothesise CHG discs will reduce catheter-associated bloodstream infection (CABSI) compared with no CHG disc. For the securement hypothesis, we hypothesise that ISD will reduce composite PICC failure (infection (CABSI/local infection), occlusion, dislodgement or thrombosis), compared with SED. Secondary outcomes: types of PICC failure; safety; costs; dressing/securement failure; dwell time; microbial colonisation; reversible PICC complications and consumer acceptability. Relative incidence rates of CABSI and PICC failure/100 devices and/1000 PICC days (with 95% CIs) will summarise treatment impact. Kaplan-Meier survival curves (and log rank Mantel-Haenszel test) will compare outcomes over time. Secondary end points will be compared between groups using parametric/non-parametric techniques; p values <0.05 will be considered to be statistically significant.Ethics and disseminationEthical approval from Queensland Health (HREC/15/QRCH/241) and Griffith University (Ref. No. 2016/063). Results will be published.Trial registrationTrial registration number is: ACTRN12616000315415.
Perception of different formats of front-of-pack nutrition labels according to sociodemographic, lifestyle and dietary factors in a French population: cross-sectional study among the NutriNet-Santé cohort participants
Authors: Julia Chantal , Péneau Sandrine , Buscail Camille , Gonzalez Rebeca , Touvier Mathilde , Hercberg Serge , Kesse-Guyot Emmanuelle 
Published: June 2017

ObjectiveFour formats for a front-of-pack (FOP) nutrition label are currently considered in France: the Nutriscore (or 5-Colour Nutrition Label, developed by a public research team), the SENS (supported by retailers), Multiple Traffic Lights (MTL, currently used in UK) and a modified version of the Reference Intakes (mRIs, supported by industry). Our objective was to investigate the perception of these FOP labels, according to sociodemographic, lifestyle and dietary factors.DesignCross-sectional study.SettingWeb-based French cohort.Main outcome measureFOP labels perception.ParticipantsParticipants in the NutriNet-Santé cohort received a specific questionnaire on the perceptions of the four label formats identified. Sociodemographic, lifestyle and dietary data (three 24-hours dietary records) were collected through self-administered questionnaires. Mutually exclusive clusters of FOP labels perception were identified through a multiple correspondence analysis followed by a hierarchical clustering procedure. Sociodemographic, lifestyle and dietary factors associated with the clusters were explored using multivariable multinomial logistic regression. All analyses were weighted according to 2009 French census data.ResultsAmong the 21,702 participants in the study, the Nutriscore received the most important number of favourable responses on positive perception dimensions by participants, followed by MTL and SENS. The five identified clusters were characterised by marked preferences for Nutriscore (cluster 1, 43.2% of participants, crude n=9,399), MTL (cluster 2, 27.3%, crude n=6,163), SENS (cluster 3, 17.05%, crude n=3,546), mRIs (cluster 4, 7.31%, crude n=1,632) and none of the presented formats (cluster 5, 5.10%, crude n=965). The cluster 1 (Nutriscore) was associated with lower adherence to nutritional recommendations, while cluster 2 (MTL) was associated with younger age and higher level of education.ConclusionThe Nutriscore appears to have a wide reach in the population and to appeal to subjects with lower adherence to nutritional recommendations.
Introducing a post-operative proforma for elective lower limb arthroplasty patients – improving patient care and junior doctor confidence
Authors: Olivier James , Stoddart Michael , Miller Katie , McLintock Robbie , Dahill Mark 
Published: June 2017

The assessment of post-operative patients is vital to identify early complications and ensure patient safety. Good clinical record keeping is essential for effective continuity of care and patient safety in the post-operative period. A group of foundation year 2 (FY2) doctors noted a disparity in levels of confidence and ability in performing this assessment.The aim of the project was to improve documentation and understanding of day one lower limb arthroplasty reviews by FY2 doctors.The Plan-Do-Study-Act model for continuous improvement was adopted from September 2015 to July 2016. A composite score comprising the twelve most important review parameters for documentation was used to score the quality of documentation on an ongoing basis. An electronic survey was completed by every FY2 rotating through the department. Interventions included registrar-led teaching sessions and an integrated review form placed in the medical notes. Further iterations of the proforma and further interventions were coordinated with the ward clerks, sisters, physiotherapists and senior clinicians.The baseline mean composite score was 6.3/12. Following implementation of a standardised proforma this score improved to 10.5 in those who had used the proforma, but 5.7 in those who hadn't. Electronic survey responses showed the proforma and teaching were effective in improving knowledge and understanding of post-operative reviews.The use of an integrated proforma in the medical notes and teaching it's use at induction, improves the documentation and understanding of day one post-operative reviews. Coordinating ward-based change across a cohort of FY2s, with involvement from the multidisciplinary team and management, affects sustained improvements in patient reviews.
Postanaesthetic pulmonary oedema in a dog following intravenous naloxone administration after upper airway surgery
Authors: Bruniges Natalie , Rigotti Clara 
Published: June 2017

A cavalier King Charles spaniel was anaesthetised for upper airway surgery. A constant rate infusion of fentanyl at 6 μg/kg/hour and top‐up boluses (5 μg/kg in total) were used for intraoperative analgesia. Intermittent positive pressure ventilation (IPPV) was instituted due to tachypnoea and inability to maintain normocapnia. Apnoea and severe hypercapnia developed after cessation of IPPV. IPPV was recommenced for 10 min to reduce hypercapnia, after which spontaneous ventilation returned. The patient had not awakened 45 minutes after isoflurane was turned off and 0.01 mg/kg naloxone was administered intravenously due to suspected fentanyl‐induced narcosis. Following immediate arousal, the patient vomited and suddenly developed symptoms and radiographic changes consistent with pulmonary oedema. General anaesthesia was reinduced and 1 mg/kg furosemide was administered intravenously. IPPV was started with application of positive end expiratory pressure in an air/oxygen mixture for 60 minutes. Recovery was uneventful. This is the first report of a dog developing pulmonary oedema following intravenous naloxone.
Repeated arterial chemoembolisation in a dog with an unresectable hepatocellular carcinoma
Authors: Fenner Joy , Caine Abby , Wray Jon , Tappin Simon 
Published: June 2017

An 11‐year‐old male neutered Staffordshire bull terrier presented for investigation of inappetence and intermittent postprandial abdominal pain. An 8 cm diameter mass was documented within the right lateral lobe of the liver. An exploratory laparotomy was performed and removal attempted however the mass was unresectable. Biopsy confirmed a low‐grade hepatocellular carcinoma. Arterial chemoembolisation was performed and the patient discharged three days later. The mass was documented to gradually reduce in size before slowly regrowing, then to remain stable until 10 months postchemoembolisation, when the patient again became inappetent. At this time the mass was 11.5 cm diameter. Repeat chemoembolisation was performed as previously. The patient developed pancreatitis following the procedure and remained hospitalised for 10 days. Five weeks after the second procedure, the mass had reduced to 8 cm in diameter. Signs of pancreatitis returned and the dog was euthanased nine weeks after the second procedure; 14 months after the first.
First-principles study of Li decorated coronene graphene
Authors: zhang Yafei , Cheng Xinlu 
Published: June 2017

We use the first-principles calculation based on density functional theory (DFT) to investigate the hydrogen storage of Li decorated coronene graphene. Our result indicates that single Li atom can adsorb three H2 molecules and the adsorption energy per H2 is −0.224 eV. When four Li atoms doped, the largest hydrogen gravimetric density is 6.82 wt.% and this is higher than the 2017 target by the US department of energy (DOE). Meanwhile, the adsorption energy per H2 is −0.220 eV, which is suitable for H2 molecules to store. Therefore, Li decorated coronene graphene will be a candidate for hydrogen storage materials in the future.
Timing of exhumation and deformation across the Taimyr fold–thrust belt: insights from apatite fission track dating and balanced cross-sections
Authors: Zhang Xiaojing , Pease Victoria , Carter Andrew , Kostuychenko Sergey , Suleymanov Arsen , Scott Robert 
Published: June 2017

Abstract
The exhumation and shortening history associated with the Taimyr fold–thrust belt is determined using apatite fission track and balanced cross-section analysis. Eighteen samples from across northern, central and southern Taimyr are used for apatite fission track analysis. These include granite, meta-arenite and sandstone samples with stratigraphic ages ranging from the late Proterozoic to Early Cretaceous. Fission track lengths and central ages are used to model the thermal history of the region and indicate three episodes of cooling in the Early Permian, earliest Triassic and Late Triassic. The thermochronological data are integrated with two balanced regional cross-sections. The regional structural style of deformation reflects a thick-skinned thrust system with 15% shortening (minimum estimate). This is consistent with thickening during early Permian Uralian orogenesis, followed by later heating, uplift and cooling associated with Siberian Trap magmatism and/or Mesozoic transpression.