bluuebunny/crossref_metadata_2025_split huggingface dataset:
ID: 1036 FAM134B, a new player in human colorectal cancer pathogenesis
Authors: Islam Farhadul , Gopalan Vinod , Lam Alfred K-Y 
Published: September 2017

Background: FAM134B (family with sequence similarity 134, member B) is a relatively new player in many human diseases including cancers. In colorectal carcinomas, FAM134B plays important role in the pathogenesis and associated with biological aggressiveness of the disease. However, expression pattern, the frequency of mutations, methylation status in colon cancer cells and tissue samples has never been studied. Also, the functional roles of FAM134B in cell have never been studied in colorectal cancer.
Objectives: To investigate FAM134B promoter methylation, mutations in tissues samples from patients with colorectal cancer and cell lines. Also, promoter methylation, expression and functional roles of FAM134B in colon cancer were studied.
Methods: Methylation and mutations in FAM134B sequence in cancer tissues (n=126) and matched non-cancer samples was studied by high-resolution melt curve analysis followed by Sanger sequencing. FAM134B expression was studied and quantified in cell lines and cancer tissues samples using immunofluorescence, immunocytochemistry, Western blot and real time PCR. In vitro functional assays and mouse xenotransplantation model were performed to unveil the molecular roles of FAM134B in colon cancer pathogenesis followed by shRNA-mediated silencing in cells.
Results: We noted that 46.5% (41/88) patients with colorectal cancer were identified as FAM134B mutations positive. Thirty-one novel pathogenic mutations were detected and these mutations were associated with gender of the patients, presence of metachronous cancer, size, T staging, presence of distant metastases and positivity of microsatellite instability (MSI) in the cancer (p < 0.05). Majority of cancer tissues had shown promoter hyper-methylation and were correlated with reduced mRNA and protein expression in both cancer samples and cells. FAM134B expression in cancer cells derived from advanced stages (stage III; SW48 and stage IV; HCT116) of colon cancer was significantly (p<0.01) reduced when compared to non-neoplastic colon cells (FHC) and cancer cells derived from stage II colon cancer (SW480). Expression of FAM134B mRNA in cancer tissues was noted significantly (p<0.001) downregulated when compared to that of non-cancer tissues samples. FAM134B suppression significantly (p<0.05) increased the proliferation of colon cancer cells, remarkably increased (3452% ; p<0.05) the clonogenic, migration capacity, and increases the proportion of cells in S phase of cell cycle (p<0.01). Xenotransplantation model showed that larger and higher grade tumors were formed in mice treated with FAM134B knockdown cells.
Conclusion: Reduced expression in cancer samples, in vitro and in vivo functional studies implied that FAM134B acts as a cancer inhibitor in colon cancer play important roles in colorectal carcinogenesis.
ID: 1074 The efficiency of adipose-derived stem cell pretreated with hepatocyte growth factor and platelet rich plasma on a liver cirrhosis mouse model
Authors: Nguyen Yen Thi-Kim , Nguyen Nam Hai , Le Trinh Van , Vo Ngoc Hong , Dang Thanh Minh , Do Huy Quang , Truong Nhung Hai 
Published: September 2017

Background: Because of their ease of isolation, high proliferation capacity in vitro, as well as their ability to differentiate into liver cells, Adipose-derived stem cell (ADSCs), are considered to be a promised candidate for liver disease treatment, including liver cirrhosis treatment. Recent studies show that hepatocyte growth factor (HGF) can stimulate ADSC to migrate to the injured areas and platelet rich plasma (PRP) can increase the stemness of ADSCs.
Method: In this study, we cultured ADSCs in the medium supplemented 20 ng/ml and 10% PRP; then transplanted them into the cirrhosis mouse model. After 11 weeks of CCl4 induction (1ml/kg, three times per week), mice were divided into 3 groups: (1) PBS group which were injected 0.2 ml PBS; (2) ADSC group which were transplanted 5x105 ADSCs non-pretreated with HGF and PRP; (3) ADSC/HGF+PRP group which were transplanted 5x105 ADSCs pretreated with HGF and PRP via the tail vein. We evaluated the effectiveness of the therapeutic at the day 7th and 14th after transplantation.
Results: The results show that the transplantation of ADSC pretreated with HGF and PRP after seven days improves the body weight (increase 4.673%); decreases the ALT level (p < 0.05), the total leukocyte number (p < 0.05) and the expression of Pro-collagen (decrease 4.1 times) as well as α-SMA (decrease 5.1 times), in comparison to the ADSC group. ADSCs pretreated with HGF and PRP also help to improve the liver tissue structure.
Conclusion: The therapy using ADSCs pretreated with HGF and PRP is considered to be a promising therapy.
ID: 1079 Improved glucose homeostasis effect of conditioned media from adipose-derived stem cells in type 1 diabetic mice
Authors: Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Phan Ngoc Kim , Dang Loan Thi-Tung 
Published: September 2017

Background: Many studies suggested adipose derived stem cell (ASC) transplantation as a new approach to control hyperglycemia in type 1 diabetes mellitus. It is proposed that the effects of these cells could be not only based on the direct cell-cell interaction but also the secretion of cytokines. This study aimed to demonstrate the effect of adipose stem cell-derived conditioned medium (CM) on the treatment of STZ-induced diabetic mice.
Methods: CM was obtained from 24-hours-cultured medium of ASCs and centrifuged to remove the debris. Type 1 diabetic mice were intraperitoneally injected CM for 30 consecutive days. Therapeutic efficacy of CM was assessed by survival rate, blood glucose level, serum insulin level, histological structure of pancreatic islets.
Results: The results showed that CM treatment could decrease mortality rate (from 33,33% to 0%) as well as blood glucose level (from 425,667±65,753 mg/dl to 203,500 mg ±20,350 mg/dl) and enhance insulin secretion, improve size and function of pancreatic islets of diabetic mice.
Conclusion: Conditioned medium maybe a promising therapy for type 1 diabetes mellitus.
ID: 1080 Adipose-derived stem cells alleviate glucose tolerance in type 2 diabetic mouse
Authors: Bui Anh Nguyen Tu , Nguyen Cong Le Thanh , Nguyen Anh Thi Minh , Truong Nhat Chau , Phan Ngoc Kim , Dang Linh Thi Tung 
Published: September 2017

Background: Type 2 diabetes (T2D) is the most common form of diabetes and accounts for 90-95% of all existing diabetic cases. The main etiologies of T2D include insulin resistance in target tissues, insufficient secretion of insulin and subsequent decline of pancreatic β-cell function. Recently, many studies have suggested that adipose – derived stem cells (ASCs) were potential to alleviate insulin resistance and hyperglycemia and promote the islets repair. In this study, ASCs were hypothesized that they could have ameliorative effects on type 2 diabetic mice.
Methods: Type 2 diabetic mice were induced by a combination of high-fat diet and injection of STZ 100 mg/kg and NA 120 mg/kg. Thereafter, two doses of 106 human ASCs were transplanted 2 week interval into each mouse via the tail vein. The mice were monitored health condition, rate of mortaity, body weight, consumption of food and water, blood glucose level, serum insulin level and histological structure of pancreatic islets.
Results: Our results indicated that the ASC-treated mice expressed improved condition in comparision with non-treated diabetic mice. The consumption of food and water as well as the blood glucose level decreased. Simultaneously, ASC transplantation improved the impaired glucose tolerance and insulin tolerance in T2D mice. Besides, the total cholesterol have significantly decreased.
Conclusion: it is suggested that human ASCs infusion is safe and effective for type 2 diabetes mellitus in mice regarding the improved glucose metabolism and insulin resistance.
Base stability of deep excavation in clay reinforced with grout piles
Authors: Su Shi-Fon , Liao Hung-Jiun 
Published: September 2017

For clay reinforced with grout piles, its shear strength is not easy to determine because the overall strength characteristic of the reinforced soil is the combined result of the shear strengths of the clay and grout piles, improvement ratio, stress path, boundary condition and so on. The purpose of this study is to develop an undrained stability analysis for the base stability of an excavation when grout piles are installed. This analysis is derived by combining the circular slip mechanism and two equivalent shear strengths: (a) the axial compression undrained shear strength of clay for the slip surface outside the excavation; (b) the axial extension undrained shear strength of clay reinforced with grout piles for the slip surface inside the excavation. This study indicates that the difference between the factor of safety calculated from the proposed method and that predicted from the anisotropic bearing capacity method is limited. However, the applicability of the proposed method to excavations with a longer depth of retaining wall (D > 50·0 m) and a narrower width of excavation (B < 0·80D) is still subject to further investigation.
Оптимизация стратегии вуза в области реализации новых программ рекрутинга студентов
Authors: Авралев Никита Владимирович , Ефимова Ирина Николаевна , Маковейчик Артем Васильевич 
Published: September 2017

<p>Данная статья посвящена исследованию эффективности комплекса профориентационных мероприятий нового формата и практики внедрения инновационных подходов в работе с абитуриентами в условиях возрастания конкуренции в сфере высшего образования в России. В числе ключевых мероприятий в комплексе — создание механизмов для развития исследовательской деятельности школьников и формирование системы непрерывного образования «школа — вуз». В статье проанализированы лучшие практики, реализуемые Национально-исследовательским Нижегородским государственным университетом им. Н. И. Лобачевского (ННГУ им. Н. И. Лобачевского) в данной области.</p><p>В качестве эмпирической базы в статье использованы локальные документы ННГУ им. Н. И. Лобачевского, посвященные реализации программы «Университетский кластер образования» и другим профориентационным мероприятиям, реализуемым в ННГУ. Также приводятся результаты социологических исследований, проведенных в университете в период летних приемных кампаний 2011–2016 гг. В целях сбора первичной информации был выбран метод анкетирования, который является достаточно эффективным, наглядным и самым распространенным способом мониторинга потребителей образовательных услуг.</p><p>К определяющим причинам, оказывающим влияние на выбор абитуриентами того или иного вуза для обучения, относятся наличие бюджетных мест на интересующих их направлениях, местонахождение университета, его материально-техническая база, научные успехи и престиж.</p><p>Сформулированы выводы и предложения по оптимизации профориентационной программы университета, реализации новых мероприятий и форматов деятельности по привлечению в университет талантливых абитуриентов. Так, главными направлениями развития здесь становятся расширение и углубление партнерских связей с ведущими школами региона, реализация совместных специальных научно-популярных мероприятий в формате опен-эйров, проведение научно-исследовательских клубов и викторин с участием высокоцитируемых ученых. </p>
Using the SSAP analysis for the primary localization of the cdt (cadmium tolerance) mutation in pea linkage group VI
Authors: Tsyganov Viktor E , Kulaeva Olga A , Knox Maggie , Borisov Aleksey U , Tikhonovich Igor A , Ellis Tomas N 
Published: September 2017

To localize the cdt mutation leading to an increased cadmium tolerance in the pea mutant and an increased cadmium accumulation in the biomass, F2 and F3 progenies from crosses between the mutant SGECdt line and the JI 281 line were analyzed. The joint inheritance of 89 SSAP (sequence specific amplified polymorphism) markers, by which the analyzed lines differed, and the mutant trait of cadmium tolerance was performed. The linkage between the trait of cadmium tolerance and 4 SSAP markers: Tps1/146+, Tps1/167+,Tps1/44+ and Tps1/58+, localized in VI pea linkage group, was shown. Thus, prospects of using SSAP analysis for primary localization of a mutation in the linkage group were demonstrated.
GENETIC DIFFERENTIATION OF SPRUCE POPULATIONS IN NORTHWEST RUSSIA REVEALED WITH MICROSATELLITE MARKERS
Authors: Potokina Elena K , Orlova Larisa V , Vishnevskaya Maria S , Alekseeva Ekaterina A , Potokin Alexander F , Egorov Alexander A 
Published: September 2017

Analysis of genetic diversity of spruce populations in northwest Russia using microsatellite markers revealed two moderately distinctive genetic clusters. One of them combined trees that were determined by morphological featuers as P. abies. The other cluster included trees identified as P. obovata and P. fennica. The SSR analysis does not support taxonomical independence of the hybrid-derived species P. fennica. Occurrence of P. abies genotypes revealed by SSR analysis significantly decreases from the south to the north.
Using of ITS sequences for the evaluation of taxonomic relations of the representatives of tribe Vicieae (Adans.) Bronn сем. Fabaceae Lindl
Authors: Ryzhova Natalia N , Burlyaeva Marina O , Kochieva E A , Vishnyakova Margarita A 
Published: September 2017

ITS1-5.8S-ITS2 nucleotide polymorphism аnalysis of Vicieae species that represent accessions of Russian genebank (VIR) collection have been performed. Nucleotide substitutions in ITS1-5.8S-ITS2-sequences and indels of different length that were species-specific or specific for Lathyrus and Viciaspecies groups have been revealed. New data proving taxonomic status of accessions with uncertain taxonomic position have been obtained.
FATE AND ECOLOGICAL INTERACTIONS OF FIREFLY LUC GENE-TAGGED SINORHIZOBIUM MELILOTI 2011-BACTERIA IN SOIL INHABITED BY HIGH LEVELS OF INDIGENOUS ALFALFA NODULATING POPULATIONS
Authors: Selbitschka Werner , Keller Mathias , Dresing Uve , Dammann-Kalinowski Tanja , Krahn Iren , Schneiker-Bekel Susanne , Sch?ffer D , Lotz W , Miethling-Graff Rona , Tebbe,  Christoph C , P?hler Alfred 
Published: September 2017

A field study was conducted with genetically modified sinorhizobium meliloti strains L1 (RecA-) and L33 (RecA+), both tagged with the firefly luciferase luc gene as an identification marker. The strains' fate was studied over a time period of five years. Both strains were rapidly outcompeted for alfalfa nodulation by an indigenous population. In summary, this study demonstrates the usefulness of tagging bacteria designed for environmental releases by the firefly luciferase gene and the high resilience of soil bacteria to allow the establishment of foreign bacterial populations. This work was supported by grants of the German Federal Ministry of Education and Research (BMBF). We thank Penny Hirsch for valuable discussion concerning the preparation of the peat-based inoculants. The contribution of the Bayer-ische Staatsministerium fur Ernahrung, Landwirtschaft und Forsten for making available the field release site in Stras-smoos is gratefully acknowledged. We thank Rudolf Beck and the team of the Strassmoos experimental plant station for their excellent support during the field study.
Molecular passportization of clones of karelian birch using PCRwith semi-specific primers
Authors: Matveeva Tatyana V , Mashkina Оlga S , Isakov Yuriy N , Lutova Ludmila A 
Published: September 2017

Using 4 clones of Karelian birch (Betula pendula Roth var caretica Merckl.) from the collection of the karelian birch of the laboratory of genetics of Research Institute of Forest Genetics and Breeding, Voronezh, one normal tree of Betula pendula Roth, and one tree of B. pubescens Ehrh., collected from the nature, we have analyzed the possibility of application of PCR with semi-specific primers for molecular typing. We found primers with high percentage of polymorphic markers. These primers could be recommended for molecular typing of birch.
Genotoxic effects of complex influence of radon and heavy metals depending on polymorphism of genes of enzymes monoxygenase systems
Authors: Minina Varvara I , Druzhinin Vladimir G , Glushkov Andrey N , Golovina Tatyana A , Apalko Svetlana V , Volkov Alexey N , Ahmatjanova Venera R , Lunina Anna A , Larionov Aleksey V 
Published: September 2017

The correlation between genotype CYP1A and level of chromosomal aberrations in adolescents which are living and learning in a boarding school of Tashtagol of Kemerovskaya oblast' in the conditions of complex genotoxic influence of raised concentration of radon and heavy metals has been studied. The levels of chromosomal aberrations were increased in carriers of at least one CYP1A1 *2A allele, as compared with CYP1A1*1A*A gomozygotes. Frequency of ring chromosomes it is significant it is raised at donors of genotype CYP1A1 *1A*1A (T3801C). Frequency of occurrence of lural chromosomal aberrations it is significant it is raised at donors with genotype CYP1A2 *1A*1A. The conclusion about the important role of CYP1A in formation genotoxic effects of complex influence of adon and heavy metals is made.
Moleсular-genetic markers in study of intra- and interspecific polymorphism of Acipenseriformes
Authors: Timoshkina Natalya N , Vodolazshky Dmitry I , Usatov Alexander V 
Published: September 2017

Uniqueness and high commercial value of relic group of sturgeon fishes stimulated researches of their genetic polymorphism. In the review the basic molekular-genetic markers used for an estimation of genetic variability are considered; their merits and demerits are discussed, examples of their application, basically, on Acipenseriformes Eurasia are resulted. Problems of the genetic analysis polyploid kinds are is short covered.
The frequencies of autoimmunity risk alleles of some genes in Belarus population
Authors: Aksenova Elena A , Pokladok Tatiana N , Boiko Dina V , Danilenko Nina G 
Published: September 2017

The population genotype and allele frequencies of +49A/G cytotoxic T-lymphocyte-associated antigen-4 (CTLA4); C1858T protein tyrosine phosphatase gene (PTPN22); –23HphIА/T insulin gene (INS) loci in native Belarusians from 6 ethnogeographic regions were estimated. The frequencies of risk allele homozygotes were: +49G CTLA4 — 17,3%; –23HphIА INS 50,7% — 1858Т PTPN22 — 4,1%. 5 individuals out of 662 investigated were risk homozygotes for all three genes, 21 were homozygotes with protective allele combination. The uniformity of genotypes and alleles distribution of investigated locuses across Belarus regions was demonstrated.
Genetic variability of plants grown on oily soils investigation by the use of ISSR-markers on Poa pratensis L. model
Authors: Boronnikova Svetlana V , Mandritsa Sergey A , Svetlakova Тatiana N , Nazarov Aleksey V , Suslonov Aleksandr V 
Published: September 2017

In this study we provide evidence of genetic variability decrease of plants grown on oily soils. Genetic variability levels of Рoa pratensis L. from oily soils were investigated by means of PCR amplification with ISSR primers. On the basis of these indicators it is confirmed, that the plants growing in the conditions of oil pollution of soils, are characterised by the lowered level of a genetic variety.
Frequency of TCR Mutant Human Lymphocytes Depending on Genotypes by Loci of Xenobiotics Detoxication
Authors: Salnikova Lubov E , Zamulaeva Irina A , Belopolskaya Olesya B , Ivanova Tatyana I , Kuznetsova Galina I , Saenko Alexander S , Abilev Serikbay K , Rubanovich Alexander V 
Published: September 2017

The results of the associative study of predisposition to an elevated somatic mutagenesis in human lymphocytes determined by the test of TCR mutant cells (CD3-CD4+phenotype) for 251 females are presented. The presence of minor alleles of polymorphic sites of CYP1A1 gene, which increase the enzyme activity, correlated with the increasing spontaneous frequency of TCR mutant cells. The analysis of gaplotypes in CYP1A1 locus (3 sites) showed that a minor gaplotype of CG sites T3801C-T606G, which had a strong effect on the parameters of somatic mutation at the frequency of around 10%, has the maximum prognostic importance relative to the studied effects.
Assessment of a state of population gene pools of terrestrial mollusks in conditions of influence of ore dressing combines on example Bradybaena fruticum Müll. (Gastropoda, Pullmonata)
Authors: Snegin Eduard A 
Published: September 2017

On the basis of analysis of the morphological and genetical variability revealed by a method of gel-electrophoresis of proteins, the state of gene pools of populations of modeling species Bradybaena fruticum (a bush snails) in conditions of influence of ore dressing combines is investigated. Authentic decrease of a level of heterozygosity and decrease of an allelic diversification in researched bunches is fixed in сomparison with the control populations, caused, both natural factors, and factors of an anthropogenic parentage. The genetic-automatic processes in populations are considered and vectors of natural selection are defined. On the basis of calculation of effective number the forecast of time of existence of populations is given.
100 years of symbiogenesis theory
Authors: Zakharov-Gezekhus Ilya A 
Published: September 2017

Konstantin Mereschkowsky suggested that the plastids originate from symbiotic cyanobacteria, and the nucleus has also originated from an endosymbiont. The hypothesis of nuclear symbiogenesis was not discussed till 1980ies. Later (Gupta et al.,1994;. Lopez-Garcia, Moreira, 2001) the data were obtained in favor of the hypothesis that the nucleus originated from an archaean captured to become an endosymbiont of a bacterial host. Short biography of Konstantin Mereschkowsky is included.
SIMULATION OF EVOLUTION OF THE LEGUME-RHIZOBIA SYMBIOSIS FOR AN IMPROVED FUNCTIONAL INTEGRITY OF PARTNERS AND FOR ECOLOGICAL EFFICIENCY OF THEIR INTERACTION
Authors: Vorobyov Nikolay I , Provorov Nikolay A 
Published: September 2017

Simulation of evolution in the legume-rhizobial symbiosis allowed us to suggest the mathematical criterion (index) for the functional integrity of symbiosis (FIS). Calculation of FIS index is based on analysis of the covariance matrix of partners’ frequencies influenced by fluctuations of the systemic parameters. The positive correlations of FIS with ecological efficiency of symbiosis (its impact on the seed productivity of plants) and a negative correlation with the partners’ diversity indices were demonstrated. The revealed dependences allow us to explain the increasing of structural-functional integrity in mutualistic symbiosis byoperation of the natural selection in favor of an improved ecological efficiency of partners’ interactions.
THE ROLE OF TRANSCRIPTION FACTORS DFOXO, DSIR2 AND HSP70 IN LIFESPAN ALTERATION OF DROSOPHILA MELANOGASTER IN DIFFERENT LIGHT CONDITIONS
Authors: Moskalev Aleksey A , Malysheva Olga A 
Published: September 2017

It was investigated the role of stress-response genes (dFOXO, dSir2, Hsp70) in regulation of life span of Drosophila in response to light regime alteration. It was revealed the FOXO-dependant mechanism of lifespan increasing at darkness conditions. The distance of lifespan of FOXO homozygous mutants at different light conditions were absent 3 times from 4 times. It was shown, that homozygotes with deletion of dSir2 have more significant difference between lifespan at standard light and darkness conditions with comparing to wild type and heterozygous strain. The same tendency was also detected the in the strains with Hsp70 deletions. It was produced the evidences of two mechanisms of light regime influence on lifespan: metabolism intensification at light conditions and neuroendocrine-determinated lifespan increasing at darkness conditions.