bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of miRNA-9 Generates Muscle Hypercontraction Through Translational Repression of Troponin-T inDrosophila melanogasterIndirect Flight Muscles
Authors: Katti Prasanna , Thimmaya Divesh , Madan Aditi , Nongthomba Upendra 
Published: September 2017

AbstractMicroRNAs (miRNAs) are small noncoding endogenous RNAs, typically 21–23 nucleotides long, that regulate gene expression, usually post-transcriptionally, by binding to the 3′-UTR of target mRNA, thus blocking translation. The expression of several miRNAs is significantly altered during cardiac hypertrophy, myocardial ischemia, fibrosis, heart failure, and other cardiac myopathies. Recent studies have implicated miRNA-9 (miR-9) in myocardial hypertrophy. However, a detailed mechanism remains obscure. In this study, we have addressed the roles of miR-9 in muscle development and function using a genetically tractable model system, the indirect flight muscles (IFMs) of Drosophila melanogaster. Bioinformatics analysis identified 135 potential miR-9a targets, of which 27 genes were associated with Drosophila muscle development. Troponin-T (TnT) was identified as major structural gene target of miR-9a. We show that flies overexpressing miR-9a in the IFMs have abnormal wing position and are flightless. These flies also exhibit a loss of muscle integrity and sarcomeric organization causing an abnormal muscle condition known as “hypercontraction.” Additionally, miR-9a overexpression resulted in the reduction of TnT protein levels while transcript levels were unaffected. Furthermore, muscle abnormalities associated with miR-9a overexpression were completely rescued by overexpression of TnT transgenes which lacked the miR-9a binding site. These findings indicate that miR-9a interacts with the 3′-UTR of the TnT mRNA and downregulates the TnT protein levels by translational repression. The reduction in TnT levels leads to a cooperative downregulation of other thin filament structural proteins. Our findings have implications for understanding the cellular pathophysiology of cardiomyopathies associated with miR-9 overexpression.
KinFin: Software for Taxon-Aware Analysis of Clustered Protein Sequences
Authors: Laetsch Dominik R , Blaxter Mark L 
Published: September 2017

Abstract
The field of comparative genomics is concerned with the study of similarities and differences between the information encoded in the genomes of organisms. A common approach is to define gene families by clustering protein sequences based on sequence similarity, and analyze protein cluster presence and absence in different species groups as a guide to biology. Due to the high dimensionality of these data, downstream analysis of protein clusters inferred from large numbers of species, or species with many genes, is nontrivial, and few solutions exist for transparent, reproducible, and customizable analyses. We present KinFin, a streamlined software solution capable of integrating data from common file formats and delivering aggregative annotation of protein clusters. KinFin delivers analyses based on systematic taxonomy of the species analyzed, or on user-defined, groupings of taxa, for example, sets based on attributes such as life history traits, organismal phenotypes, or competing phylogenetic hypotheses. Results are reported through graphical and detailed text output files. We illustrate the utility of the KinFin pipeline by addressing questions regarding the biology of filarial nematodes, which include parasites of veterinary and medical importance. We resolve the phylogenetic relationships between the species and explore functional annotation of proteins in clusters in key lineages and between custom taxon sets, identifying gene families of interest. KinFin can easily be integrated into existing comparative genomic workflows, and promotes transparent and reproducible analysis of clustered protein data.
THE EFFECT OF MASSAGE STIMULATION TO REDUCTION OF TUMOR NECROTIC FACTOR-ALFA (TNF-Î±) AND INTERLEUKIN-6 (IL-6) IN PRETERM, LOW BIRTH WEIGHT APPROPRIATE WITH GESTATIONAL AGE INFANTS
Authors: Trisna-Windiani I G. A. , Soetjiningsih Soetjiningsih , Mantik-Astawa N. , Rusmil Kusnandi 
Published: September 2017

Preterm condition gives a new stressful environment to infants. This condition needs a good and gradual adaptation. External environmental conditions, treatment, care and good nutrition are needed. Massage stimulation in preterm infants is a method that can prepare preterm infantsâ€™ adaptation through the critical post-birth period. A good adaptation of the system will provide better organ homeostasis including the immune system.There are three paths of mechanisms that can explain how massage stimulation can regulate pro-inflammatory cytokines TNF-Î± and IL-6. The first: the hypothalamus pathway, massage stimulation act as a comfort stimulation that can control (inhibit) the release of pro-inflammatory cytokines TNF-Î±Â and IL-6 by macrophages; second: through activation of vagus nerve, massage stimulation act as a tactile stimulation which activates vagal system and the immune system; third: the hypothalamic-pituitary pathway, massage stimulation act as a kinesthetic stimulation that activates the hypothalamic-pituitary to release cortisol, GH (Growth hormone) and IGF-1 (Insulin-like Growth Factor 1). A randomized controlled trial about massage stimulation on preterm infants was performed in Sanglah Hospital Denpasar on 2011. The study revealed a different level of cytokine pro-inflammation TNF-Î± (p=0.025) and IL-6 (p=0,001) comparing before and after condition massage stimulation. Outcome of body weight were also different at before and after massage stimulation, with p value 0.042.Mechanisms of massage stimulation affect the immune system of preterm infants through regulation of inhibition the release of pro-inflammatory cytokine TNF-Î±, IL-6 by macrophages, through three main pathways: the hypothalamic; activation of the vagus nerve and the hypothalamic-pituitary-adrenal axis.
INCREASING LEVELS OF INTERLEUKIN-1ï¢, INTERLEUKIN-1 RECEPTOR ANTAGONIST IN BALINESE IDIOPATHIC GRANDMAL EPILEPSY
Authors: Anna-Marita Gelgel , Sudewa-Djelantik A. A. G. , Purwata Thomas Eko , Junitha Ketut 
Published: September 2017

Purpose. To asses the level of proinflammatory and anti inflammatory cytokines in Balinese children with idiopathic grandmal epilepsy (IGE)Â Method and Results.We studied 25 Â patients with idiopathic grandmal epilepsy aged.., (and control with match age and sex) admitted to. In each patient, serum levels of interleukin-1 b and interleukin-1 receptor antagonist were measured at entry and compare to healthy control.In group case, mean IL-1 v and IL-1RA. In control group;Â Conclusions.This finding may suggest novel therapeutic approaches to children with IGE
ANALISIS ALIRAN DAYA UNTUK PENENTUAN LOKASI PENYALURAN DAYA PLTN DI SISTEM KALIMANTAN BARAT
Authors: Candranurani Citra , Setya Budi Rizki Firmansyah , Lumbanraja Sahala Maruli 
Published: September 2017

ANALISIS ALIRAN DAYA UNTUK PENENTUAN LOKASI PENYALURAN DAYA PLTN DI SISTEM KALIMANTAN BARAT. Kondisi kelistrikan di Kalbar diketahui dalam situasi krisis akibat jumlah kapasitas daya pembangkit yang hampir sama dengan beban puncak. Sistem tidak memiliki cadangan pembangkitan sehingga mengakibatkan defisit listrik ketika ada pembangkit yang tidak beroperasi. Kebijakan perencanaan listrik hingga tahun 2022 adalah dengan membangun PLTU untuk mengganti PLTD yang telah ada. Untuk perencanaan jangka panjang diperlukan peranan energi baru terbarukan guna mengurangi ketergantungan pemakaian bahan bakar fosil, diantaranya adalah pemanfaatan PLTN. Aspek kelistrikan, salah satunya untuk mengetahui lokasi penyaluran daya PLTN yang optimum, maka diperlukan analisis aliran daya. Lokasi Gardu Induk (GI) yang dianalisis adalah keseluruhan GI di Kalbar yaitu sejumlah 20 unit. Metoda perhitungan aliran daya pada penelitian ini menggunakan perangkat lunak ETAP 12.5. Perencanaan pengoperasian PLTN digunakan untuk memikul beban dasar, sehingga agar optimum maka faktor kapasitasnya diatas 80%. Hasil penelitian menunjukkan 3 lokasi yang dapat membangkitkan daya diatas 80%, yaitu: GI Mempawah, GI Singkawang, dan GI Sambas. Lokasi paling optimum berada di GI Mempawah dengan faktor kapasitas 83,5%. Letak ketiga GI ini sejalan dengan salah satu syarat untuk pembangunan PLTN, yaitu tersedianya sumber air pendingin karena lokasi tersebut dekat dengan pinggir pantai. Kata kunci: Aliran daya, lokasi optimum, PLTN, Kalimantan Barat
Language Beliefs among Adult Learners: Individual Differences
Authors: Johari Aiza 
Published: September 2017

To develop the quality of language learning and utilise learning opportunities in and out of a classroom, it is vital to understand individual's beliefs in language learning. White (2008) affirmed that an individual’s belief plays a key role in the learning process and language acquisition, especially the beliefs the learners have about themselves, language and language learning, and about the settings in which they contribute as language learners and users. This study identifies the English language learning beliefs of adult learners (between 45-50 years of age) in a local university in Sarawak. Questionnaire was the instrument to collect the data and the data were analysed using SPSS Version 21. The study showed that these adult learners have various levels of language beliefs. The results obtained can be used to enhance the teaching and learning of English language where language instructors can learn more about their learners and the learners’ language beliefs can further enhance learning process in the classroom.
Efforts to resolve the problem of forest area conservation on the national park in the island of Sumatra
Authors: Desmiwati NFN , Surati NFN 
Published: September 2017

In the frame of the implementation of good forest management, the certainty of the factually and legally forest area it is needed in order in order to have solid legal foundation. This means the stability on forest boundary areas it is required. This study aimed to carry out scrutiny of the stability of 11 national parks areas in Sumatra, and to recognize the development of stabilization of national parks. The study is conducted in 11 national parks in Sumatra consisting of 7 national parks as conservation forest management unit (CFMU) and 4 parks are not CFMU. The analysis approach used for the research was qualitative descriptive. The results showed that out of 11 national parks only 27.27% which has been structuring the outer boundary from end to end, meanwhile 72.72% have not done. This is because there are some boundaries areas that will be regulated still have problems with the community. Zonation structuring has been conducted by 10 National Park (90.91%) but 1 TN (9.09 have not done the zonation structuring yet). There are some national parks has not been define the boundary properly. Out of number of issues, some efforts that need to be done are to clarify boundary markers on the national parks that can be recognized by all stakeholders, the the acceleration of zone marking and carried out in accordance with the interests of all stakeholders, as well as the need for community involvement in the management of national parks.
Técnicas para el aislamiento, identificación y caracterización molecular de cepas de Ralstonia solanacearum relacionadas a Moko del plátano
Authors: Obrador-Sánchez José Abraham , Tzec-Simá Miguel , Canto-Canché Blondy , Higuera-Ciapara Inocencio 
Published: September 2017

<p><em>Ralstonia solanacearum</em> es uno de los fitopatógenos más destructivos en la agricultura; en el cultivo de plátano la raza 2 causa la enfermedad denominada Moko del plátano, la cual en México está restringida a algunas áreas y provoca pérdidas cuando ocurren brotes epidémicos. La raza 2 comprende subgrupos (secuevares), los cuales usualmente son identificados mediante PCR de tipo Multiplex. El aislamiento de <em>R. solanacearum</em> es usualmente difícil, aún sobre medios semiselectivos, debido a su crecimiento lento y a la frecuente contaminación con bacterias <em>Pseudomonas</em>,<em> Klebsiella </em>y<em> Erwinia</em>,<em> </em>que presentan fenotipos similares<em>. </em>En este trabajo se presenta una estrategia que facilita el aislamiento, identificación y clasificación de <em>R. solanacearum</em> raza 2. El protocolo comprende inmunodiagnóstico del material vegetal sospechoso, confirmación mediante PCR, aislamiento sobre medios semiselectivos SMSA y B-King, y genotipificación de los secuevares mediante reacciones de PCR sencillas, específicas. En el presente trabajo se aislaron 25 cepas de muestras obtenidas del estado de Tabasco, las cuales genotipificaron en el secuevar 6. Su previa genotipificación mediante Multiplex fue confusa, lo que evidencia que ese ensayo puede llevar a conclusiones erróneas. Por lo tanto no es válido proponer divergencia genética y emergencia de nuevas cepas con base en su resultado, como se ha hecho en algunos reportes recientes.</p>
Portfolio Selection with Random Liability and Affine Interest Rate in the Mean-Variance Framework
Authors: Chang Hao , Wang Chunfeng , Fang Zhenming 
Published: September 2017

Abstract
This paper studies a dynamic mean-variance portfolio selection problem with random liability in the affine interest rate environment, where the financial market consists of three assets: one risk-free asset, one risky asset and one zero-coupon bond. Assume that short rate is driven by affine interest rate model and liability process is described by the drifted Brownian motion, in addition, stock price dynamics is affected by interest rate dynamics. The investors expect to look for an optimal strategy to minimize the variance of the terminal surplus for a given expected terminal surplus. The efficient strategy and the efficient frontier are explicitly obtained by applying dynamic programming principle and Lagrange duality theorem. A numerical example is given to illustrate our results and some economic implications are analyzed.
A Reversed Geo–Dynamic Approach for Brownfield Rejuvenation
Authors: Ibrahim Mahmoud (Mudi) , Hollmann Gregor 
Published: September 2017

Summary
Brownfields in this paper are defined as mature fields where production declined to less than 35–40% of the plateau rate and where primary and secondary reserves have been largely depleted. Big data, high field complexity after a long production history, and slim economic margins are typical brownfield challenges.
In the exploration-and-production (E&P) industry, “sequential” field-evaluation approaches (first “static,” then “dynamic”), have proved successful for greenfield development, but often do not achieve satisfying results for brownfields. This paper presents a new work flow for brownfield re-evaluation and rejuvenation. The “reversed” geo-dynamic field modeling (GDFM) rearranges existing elements of reservoir evaluation to obtain a purpose-driven, deterministic reservoir model, which can be quickly translated into development scenarios.
The GDFM work flow is novel because (1) it turns upside down the discipline-driven sequential work flow (i.e., starts with the history match) and (2) it uses dynamic data as input to calibrate seismic (re-) interpretation that acts as a main integration step. It combines all available data already during horizon and fault mapping. Field diagnosis, flow-unit identification, well-test reanalysis, and petrophysical and geological interpretations are all combined in a cross-discipline interaction to guarantee data consistency. This directly ensures a fully integrated, “geo-dynamic” model that forms the basis for reservoir modeling. The full dynamic/static data coupling at an early stage is the main strength of the GDFM. It reduces the model complexity, and narrows the uncertainties. Project-execution time is considerably shortened by avoidance of the characteristic full-cycle loop iterations of the sequential approaches.
A brownfield example illustrates the benefits of GDFM: a consistent history match with high model accuracy and confidence. In the field example, the GDFM work flow has facilitated a turnover at only 70% of the original time budget. The ongoing drilling has confirmed model validity (“attic oil” predictions), thus further postponing the economic limit of the brownfield.
Anais do 1º Encontro de Revisão Sistemática e/ou Meta-Análise do Programa de Pós-Graduação em Odontologia FOA/UNESP
Authors: Pellizzer Eduardo Piza 
Published: September 2017

As revisões sistemáticas surgiram no final da década de 1950, porém, somente no final da década de 1980 se consolidou na área da saúde.Frequentemente nos deparamos com estudos contraditórios, com amostragem e metodologia diferentes, estudos de laboratórios, simulações e relato de casos, entre outros. Estes possuem rigor e qualidades diferentes, tornando-se difícil a interpretação isolada, com o objetivo de aplicabilidade clinica. Muitas vezes estes estudos podem ser tendenciosos, e refletem a opinião de um especialista ou um grupo de pesquisadores; que levando em consideração a pirâmide de evidencia científica (visto que são a base da pirâmide), não deve ser levado em consideração.Portanto nos dias atuais, principalmente na área da saúde, o ensino nas universidades e as condutas clinicas adotadas pelos profissionais necessitam ser apoiadas em evidencias cientificas. Nesse sentido, destacamos a importância das revisões que se baseiam em métodos científicos rigorosos, conduzidas geralmente com estudos clínicos randomizados e que chegam a uma conclusão mais efetiva sobre o tema abordado. E quando a maioria desses estudos apresentam dados homogêneos, podemos aplicar uma analise estatística e transforma-la em uma metanálise.Com o objetivo de conscientizar e divulgar a importância desses estudos, criamos um Encontro Anual do Grupo de Revisão Sistemática e/ou Metanálise do Programa de Pós-Graduação em Odontologia da Faculdade de Odontologia de Araçatuba, que ocorrera todos os anos no mês de dezembro, aberto a toda a comunidade odontológica. Prof.Titular Eduardo Piza PellizzerCoordenador do 1º Encontro de Revisão Sistemática e/ou Meta-Análise do Programa de Pós-Graduação em Odontologia FOA/UNESP
Dosis óptima de irradiación gamma con Co60 para inducción de mutagénesis en pastos
Authors: Álvarez-Holguín Alan , Corrales-Lerma Raúl , Morales-Nieto Carlos Raúl , Avendaño-Arrazate Carlos Hugo , Villarreal-Guerrero Federico 
Published: September 2017

La dosis letal (DL50) y reducción media del crecimiento (RC50) son parámetros utilizados para seleccionar dosis de irradiación adecuadas e inducir mutaciones para realizar mejoramiento genético vegetal. El objetivo fue determinar la DL50 yRC50 para conocer la dosis ótima de irradiación con Co60 en semilla de pasto llorón, buffel, banderita y navajita. Se evaluaron seis dosis de irradiación con Co60 en semilla: 100, 200, 300, 450, 600 y 900 Gray (Gy) y un tratamiento control (T-0; sin irradiación). Las variables evaluadas fueron porcentaje de germinación (%GE), índice de velocidad de germinación (IVG), longitud de plúmula (LP) y longitud de radícula (LR). Se realizó análisis de regresión y comparación de medias con la prueba de Dunnett. Mediante la ecuación de regresión se estimó la DL50 para el %GE, así como RC50 para el resto de las variables. La DL50 se ponderó con 55%, mientras que la RC50 se ponderó en 15% para cada una de las tres variables restantes. En general, los valores de las variables disminuyeron (P<0.05) conforme se incrementó la dosis de irradiación, lo cual permitió determinar la DL50 y RC50 para cada especie. La media ponderada se obtuvo a los 628 Gy, 712 Gy, 698 Gy y 411 Gy para pasto llorón, buffel, banderita y navajita, respectivamente. Se recomiendan las dosis planteadas en este estudio, ya que esto permitirá plantear las bases para realizar mejoramiento genético a través de mutagénesis con irradiación gamma en los pastos evaluados.
Assessing the complex adaptability of regional water security systems based on a unified co-evolutionary model
Authors: Yao Jiping , Ren Yongtai , Wei Shuai , Pei Wei 
Published: September 2017

Abstract
A unified co-evolutionary model was developed to study the adaptability conditions of regional water security systems, which is important for the coordinated development of these systems. In this work, the main factors that affect the adaptability of regional water security systems, the contribution of each sub-problem domain to the development of the problem domain, and the fitness values of regional water security systems were analyzed based on the model. Taking Jiansanjiang as an example, the results showed that in 2002–2011, the water resources system had strong adaptability and contributed greatly to improve the adaptability of the water security system; the socioeconomic system had poor adaptability to environmental changes and contributed little to the adaptability of the water security system; and the eco-environmental system was barely able to adapt to the changing environment and contributed less to the adaptability of the water security system. Due to the influence of the socioeconomic and eco-environmental systems, the adaptability of the water security system was relatively weak. Therefore, strengthening the sustainable utilization of water resources, promoting the coordinated development of the social economy, and improving the quality of the ecological environment are effective strategies to improve the adaptability of water security systems.
Multicriteria performance analysis of an integrated urban wastewater system for energy management
Authors: Puleo Valeria , Notaro Vincenza , Freni Gabriele , La Loggia Goffredo 
Published: September 2017

AbstractThe optimization and management of an integrated urban wastewater system is a complex problem involving many processes and variables. The possible control options are defined by several management strategies that may differently impact the economic, operational or environmental performance of the system. The present paper aims to contribute to the environmental and energy sustainability of urban wastewater systems by means of a multicriteria performance analysis. The paper begins with a complete analysis of the system performance in several fields of interest (energy, environment, quality of service, operation, economy and financial resources), and it highlights the management strengths and weaknesses in each subsystem. The analysis was carried out by means of a prototype, developed during the ALADIN project, which enables understanding the system, planning effective improvement actions and assessing their possible effects in each part of the urban water cycle. To demonstrate the potential of such an approach, it was tested on an actual integrated urban wastewater system in Sicily.