bluuebunny/crossref_metadata_2025_split huggingface dataset:
Etnobotani dan Distribusi Jenis Tumbuhan Berkhasiat Obat Pada Masyarakat Desa Candipuro, Kecamatan Candipuro, Kabupaten Lumajang
Authors: Putri Dheajeng Aprelia , Hayati Ari , Lisminingsih Ratna Djuniwati 
Published: September 2024

Ethnobotany is the study of information about how ethnic groups, tribes, communities, and their environment historically or locally use and manage plants. The purpose of this study was to obtain data on plant species, plant benefits, plant parts that are utilized and how to process plants as medicinal plants by the community in Candipuro Village, Candipuro District, Lumajang Regency. Semi-structured interviews with respondents who know or have used medicinal plants were used to collect data on medicinal plants. The selection of respondents was carried out using the Snowball Sampling technique, namely by identifying Key Person (Key Figure). People in Candipuro village use 32 plant species from 19 families, each of which has benefits in curing diseases. Plant parts such as tubers, rhizomes, leaves, flower fruits, bark, seeds, gels, and fronds are utilized or processed as ABSTRAKEtnobotani merupakan studi tentang informasi mengenai bagaimana kelompok etnis, suku, masyarakat, dan lingkungannya secara historis atau lokal menggunakan dan mengelola tumbuhan. Tujuan penelitian ini adalah untuk memperoleh data mengenai jenis tumbuhan, manfaat tumbuhan, bagian tumbuhan yang dimanfaatkan serta cara mengolah tumbuhan sebagai tumbuhan berkhasiat obat oleh masyarakat di Desa Candipuro, Kecamatan Candipuro, Kabupaten Lumajang. Wawancara semi-terstruktur dengan responden yang mengenal atau pernah menggunakan tumbuhan obat digunakan untuk mengumpulkan data tentang tumbuhan obat. Pemilihan responden dilakukan dengan teknik Snowball Sampling, yaitu dengan mengidentifikasi Key Person (Tokoh Kunci). Masyarakat di desa Candipuro menggunakan 32 spesies tumbuhan dari 19 familia yang mana masing masing tumbuhan memiliki manfaat dalam menyembuhkan penyakit. Bagian tumbuhan seperti umbi, rimpang, daun, buah bunga, kulit batang, biji, gel, dan pelepah dimanfaatkan atau diolah sebagai tumbuhan obat. Masyarakat menggunakan cara merebus, memotong, menumbuk, mengukus, memeras, dan mengonsumsi langsung sebagai metode pengolahan tumbuhan obat. Persebaran Tumbuhan Berkhasiat Obat pada 4 dusun yang ada di Desa Candipuro, yaitu pada Dusun Krajan didapatkan jumlah frekuensi sebesar 72% sedangkan pada Dusun Candi wetan sebesar 44%, pada Dusun Panggung Lombok lor dan Dusun Panggung Lombok kidul sebesar 62%. Kata kunci: Etnobotani, Tumbuhan Berkhasiat Obat, Distribusi, Pemanfaatan.
Analisis Limbah Cair Industri Tahu Sebelum Dan Sesudah Penggunaan Konsorsium Effective Microorganism 4 (EM4)
Authors: Jannah Nurwatul , Lisminingsih Ratna Djuniwati , Syauqi Ahmad 
Published: September 2024

There are many tofu factories in Indonesia and this causes a lot of waste to be generated. On average, tofu factories are unable to process waste, so the waste produced is thrown directly into the environment without processing, so it can pollute the environment. Liquid waste processing can be done simply, namely by bioremediation using EM4. This research aims to compare between control treatments, EM4 7.5% and EM4 15% in the processing of industrial tofu liquid waste. The research method used True Experiment Design and Completely Randomized Design (CRD), with 3 treatments namely P1 (control), P2 (EM4 7.5%), and P3 (EM4 15%). Each treatment was repeated 7 times. The research parameters measured were pH, BOD, COD, and TSS before and after treatment. The results of the data obtained during the observation were analyzed using the ANOVA test then tested by LSD test. The research results showed that there were differences in bioremediation after using Effective microorganism-4 (EM4) in the control treatment, EM4 7,5% and EM4 15%, but it was not yet effective in reducing BOD, COD and TSS levels in tofu wastewater. The treatment has not been effective because the remediation results still did not meet the quality standarts in East Java Governor Regulation No 52 of 2014. Keywords: tofu wastewater, EM4, BOD, COD, TSS.
Studi Pertumbuhan Bawang Merah (Allium ascalonicum L.) pada Media Hidroponik dengan Pupuk Organik Limbah Sayur
Authors: Septianing Eka , Hayati Ari , Lisminingsih Ratna Djuniwati 
Published: September 2024

This research aims to determine the effect of organic fertilizer from vegetable waste on the growth of shallots (Allium ascalonicum L.) and the optimal concentration of organic fertilizer from vegetable waste on the growth of shallots (Allium ascalonicum L.) in a hydroponic system. This research method was carried out using a Completely Randomized (CRD) design using 5 treatment levels with 5 repetitions using concentrations of 0%, 25%, 50%, 75% and 100%. The parameters used are the number of leaves (strands), length of leaves and roots (cm) and number of roots. If there is an effect, then a further DMRT test is carried out. The results of statistical analysis showed that there were no differences in the number of leaves and leaf length in the treatments, but there were differences in the number of roots and root length in the treatments. The results of the DMRT test on root number and root length showed that the control treatment (0% POC) was the concentration that produced the highest root length and root number. The results of liquid organic fertilizer from vegetable waste have a significant influence on the growth in length and number of roots of shallots (Allium ascalonicum L.). ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pupuk organik dari limbah sayur terhadap pertumbuhan bawang merah (Allium ascalonicum L.) dan konsentrasi pupuk organik limbah sayur yang optimal terhadap pertumbuhan bawang merah (Allium ascalonicum L.) pada sistem hidroponik. Metode penelitian ini dilakukan menggunakan rancangan Acak Lengkap (RAL) menggunakan 5 taraf perlakuan 5 kali pengulangan menggunakan konsetrasi 0%, 25%, 50%, 75%, dan 100%. Parameter yang digunakan adalah jumlah daun (helai), panjang daun dan akar (cm) serta jumlah akar. Jika terdapat pengaruh, maka dilakukan uji lanjut DMRT. Hasil analisis statistik menunjukkan bahwa tidak ada perbedaan jumlah daun dan panjang daun pada perlakuan, tetapi terdapat perbedaan jumlah akar dan panjang akar pada perlakuan. Hasil uji DMRT terhadap jumlah akar dan panjang akar menunjukan bahwa perlakuan kontrol (0% POC) merupakan konsetrasi yang menghasilkan panjang akar dan jumlah akar tertinggi. Hasil pupuk organik cair limbah sayur memiliki pengaruh yang signifikan terhadap pertumbuhan panjang dan jumlah akar bawang merah (Allium ascalonicum L.).Kata kunci: Bawang merah, hidroponik, limbah sayur, pupuk organik dan pertumbuhan bawang merah
Seasonal variation of total and bioavailable 25-hydroxyvitamin D [25(OH)D] in the healthy adult Slovenian population
Authors: Osredkar Joško , Vičič Vid , Hribar Maša , Benedik Evgen , Siuka Darko , Jerin Aleš , Čegovnik Primožič Urška , Fabjan Teja , Kumer Kristina , Pravst Igor , Žmitek Katja 
Published: September 2024

Objective: The aim of our study was to compare the total 25(OH)D fraction, the bioavailable vitamin fraction, and the free vitamin D fraction in spring and fall in a group of healthy individuals.Methods: In our study, we collected blood samples from healthy participants at the end of both summer and winter, and measured serum levels of albumin, DBP, and 25(OH)D. Utilizing these data, we calculated the percentage of free and bioavailable vitamin D. Our cohort comprised 87 participants, with a male-to-female ratio of 14:73, aged 35.95 ± 12.55 years, ranging from 19 to 70 years. We employed the chemiluminescence method to determine the vitamin 25(OH)D levels, the ELISA method was utilized to determine DBP levels, the albumin BCP Assay was performed using the ADVIA biochemical analyzer (Siemens) and an online calculator was used to determine the free and bioavailable 25(OH)D levels.Results: Our findings indicate significantly lower 25(OH)D levels in winter (44.13 ± 17.82 nmol/L) compared to summer (74.97 ± 22.75 nmol/L; p &lt; 0.001). For vitamin D binding protein there was no significant difference from summer (236.2 ± 164.39 mg/L) to winter (239.86 ± 141.9 mg/L; p = 0.77), albumin levels were significantly higher in summer (49.37 ± 4.15 g/L vs. 47.97 ± 3.91 g/L, p = 0.01), but the magnitude of the change may not be large enough to be solely responsible for the stability of vitamin D levels throughout the year. In the winter season a significantly lower calculated bioavailable 25(OH)D vitamin (7.45 ± 5.66 nmol/L against 13.11 ± 8.27 nmol/L; p &lt; 0.001) was observed, and the free fraction also showed a significant decrease (17.3 ± 12.9 pmol/L versus 29.7 ± 19.1 pmol/L; p &lt; 0.0001). We observed a moderately positive correlation between 25(OH)D and bioavailable percentage in winter (r = 0.680; p &lt; 0.001), in contrast with a lower positive association in summer (r = 0.343; p &lt; 0.001).Conclusion: Our data suggest a positive correlation between total and bioavailable 25(OH)D levels. In addition to the statistically significant variation in 25(OH)D between the two observation periods, there was an additional variation in the free vitamin D percentage. The summertime synthesis of vitamin D in the skin could contribute directly to the free fraction of vitamin D. Standardizing the measurement of free 25(OH)D and clinical studies is necessary to establish reference values before these methods can be implemented in clinical practice.
A novel ultrasound approach in assessing IMAT in critically ill patients
Authors: Molinger Jeroen , Whittle John , Endeman Henrik , MacLeod David , Gupta Rajan , Fudim Marat , Haines Krista L. , Barkauskas Christina , Healy Zachary , Wischmeyer Paul , Bakker Jan 
Published: September 2024

BackgroundMuscle wasting is a common finding in critically ill patients associated with increased days of mechanical ventilation in the ICU. Muscle wasting and associated morphological changes are hallmarks of ICU-acquired weakness. Muscle wasting can be diagnosed and quantified by muscle biopsy, but biopsies can cause multiple adverse effects. MuscleSound® has developed a non-invasive, real-time novel MusculoSkeletal (MSK) ultrasound approach to measure the ratio between percentage intramuscular adipose tissue (%IMAT) with muscle cross-section area (MCSA) (termed IMAT-Index). The present study aimed to assess the IMAT-Index in ICU patients longitudinally and compare it to age-matched healthy controls.MethodsTransverse (short-axis) ultrasound images of the rectus femoris muscle were obtained upon admission and discharge in 35 ICU patients and compared to age-matched healthy controls (n = 975). The echo intensity of the image taken from the muscle is used to automatically calculate the ratio between intramuscular adipose tissue by cm2 (IMAT-Index), the cross-sectional area of the muscle (MCSA), and muscle thickness (MT).ResultsIMAT-Index was successfully measured in all subjects. The mean IMAT-index (%IMAT/cm2) upon admission was significantly higher in critically ill patients compared to healthy controls (7.4 ± 4.3 vs. 3.1 ± 0.9, p &lt; 0.001). At ICU discharge, the IMAT-index increased in the ICU group compared to admission (8.1 ± 3.5 vs. 7.4 ± 4.3, p &lt; 0.05).ConclusionReal-time noninvasive MSK ultrasound IMAT-Index was higher in ICU patients than in healthy controls and increased during the ICU stay, both in male and female patients.
Heart failure biomarkers in revascularized patients with stable coronary heart disease as clinical outcome predictors
Authors: Bošnjak Ivica , Bedeković Dražen , Selthofer-Relatić Kristina , Roguljić Hrvoje , Mihaljević Ivica , Dukić Darko , Bilić-Ćurčić Ines 
Published: September 2024

IntroductionThe aim of this study was to investigate serum levels of galectin-3 (Gal-3) and N-terminal pro-brain Natriuretic Peptide (NT-proBNP) in patients with stable obstructive coronary artery disease, as well as their potential to predict clinical outcomes.MethodsThis was a single-center cross-sectional cohort study. 168 patients were divided into three groups: percutaneous coronary intervention (PCI) group (N 64), coronary artery bypass graft surgery (CABG) group (N 57), and group with no coronary stenosis (N 47). Gal-3 and NT-proBNP levels were measured and the Syntax score (Ss) was calculated.ResultsThe mean value of Gal-3 was 19.98 ng/ml and 9.51 ng/ml (p &lt; 0.001) in the study group and control group, respectively. Highest value of Gal-3 was found in the group of subjects with three-vessel disease (p &lt; 0.001). The mean value of NT-proBNP in the study group was 401.3 pg/ml, and in the control group 100.3 pg/ml (p = 0.159). The highest value of NT-proBNP was found in the group of subjects with three-vessel disease (p = 0.021). There was a statistically significant association between Gal-3, NT-proBNP and occurrence of adverse cardiovascular event (p = 0.0018; p = 0.0019).ConclusionGal-3 and NT-proBNP could be used as an additional tool for diagnosis and severity assessment of stable obstructive coronary artery disease. Furthermore, it could help identify high-risk patients who could experience major adverse cardiovascular events.
Assessment of microphytobenthos communities in the Kinzig catchment using photosynthesis-related traits, digital light microscopy and 18S-V9 amplicon sequencing
Authors: Mayombo Ntambwe Albert Serge , Dani Mimoza , Kloster Michael , Vidaković Danijela , Buchner Dominik , Burfeid-Castellanos Andrea M. , Beszteri Bánk 
Published: September 2024

IntroductionMicroalgae form an essential group of benthic organisms that respond swiftly to environmental changes. They are widely used as bioindicators of anthropogenic stressors in freshwater ecosystems. We aimed to assess the responses of microalgae communities to multiple environmental stressors in the Kinzig River catchment, home to a long-term ecological monitoring site, in Germany.MethodsWe used a photosynthetic biomass proxy alongside community composition of diatoms assessed by digital light microscopy, and of microalgae by 18S-V9 amplicon sequencing, to characterise microalgae at 19 sampling sites scattered across the catchment.ResultsOur results revealed significant effects of physical and chemical factors on microalgae biomass and community compositions. We found that conductivity, water temperature and pH were the most important factors affecting microalgae community composition, as observed in both microscopy and amplicon analysis. In addition to these three variables, the effect of total phosphate on all microalgae, together with water discharge on the diatom (Bacillariophyta) communities, as assessed by amplicon analysis, may reveal taxon-specific variations in the ecological responses of different microalgal groups.DiscussionOur results highlighted the complex relationship between various environmental variables and microalgae biomass and community composition. Further investigations, involving the collection of time series data, are required to fully understand the underlying biotic and abiotic parameters that influence these microalgae communities.
Increased incidence of seronegative autoimmune hepatitis in children during SARS-CoV-2 pandemia period
Authors: Schmutz Muriel , Chartier Suzanne , Leblanc Thierry , Mussini Charlotte , Gardin Antoine , Gonzales Emmanuel , Roque-Afonso Anne-Marie , Le Cam Solene , Hery Geraldine , Neven Benedicte , Charbel Ramy , Vartanian Jean-Pierre , Jacquemin Emmanuel , Morelle Guillaume , Almes Marion 
Published: September 2024

BackgroundSeronegative autoimmune hepatitis in children is a rare but potentially severe disease, sometimes requiring liver transplantation. This type of hepatitis may be associated with various immunological and hematological disorders, ranging from isolated lymphopenia to aplastic anemia. Precise pathophysiological mechanisms are still unknown, but the role of viruses cannot be excluded, either as directly pathogenic or as triggers, responsible for an inappropriate immune stimulation. Having the impression of an increasing number of seronegative autoimmune hepatitis since the beginning of SARS-CoV-2 pandemia period, we hypothesized that SARS-CoV-2 virus could be an infectious trigger.MethodsWe conducted a retrospective, observational, descriptive study about children with seronegative autoimmune hepatitis, in a tertiary care center, between 2010 and 2022.ResultsThirty-two patients were included. The overall incidence of seronegative autoimmune hepatitis increased 3.3-fold in 2020-2022, during the SARS-CoV-2 pandemia period (16 patients in 2.8 years) compared with 2010-2019 the pre pandemia period (16 patients in 9 years). Patients’ clinical and biochemical liver characteristics did not differ between the two periods. Hematological damages were less severe during the pandemia period. Immunological studies revealed a dysregulated immune response. The initiation of immunosuppressive therapy (corticosteroids ± cyclosporine) was earlier during the pandemia period than before.ConclusionIn cases of undetermined acute hepatitis, an immune-mediated origin should be considered, prompting a liver biopsy. If the histological aspect points to an immune origin, immunosuppressive treatment should be instituted even though autoimmune hepatitis antibodies are negative. Close hematological monitoring must be performed in all cases. The 3.3-fold increase of cases during the SARS-CoV-2 pandemia will need to be further analyzed to better understand the underlying immunological mechanisms, and to prove its potential involvement.
Impact of the pandemic on hospital care for chronic pain patients in Germany
Authors: Cegla Thomas , Hohenstein Sven , Bollmann Andreas , Pellissier Vincent , Bencheva Veronika , Schmiedl Sven 
Published: September 2024

ObjectivesThe COVID-19 pandemic affected patients’ access to health services, including patients with severe chronic pain. Since limited data on pandemic-caused changes in pain therapy is available, we analyzed its effect on hospital-based pain treatment.MethodsFor this retrospective claims data analysis conducted in n = 37 hospitals, we included patients treated for a chronic pain-related diagnosis. Discharge rates stratified by region and pain unit size were analyzed for different time periods between January 2019 and June 2022.ResultsThere was a significant decrease in day-care, inpatient interdisciplinary multimodal pain management, from a total of 5,533 hospital pre-pandemic treatments in 2019, to 3,942 in 2020 and 4,262 in 2021, with a slight increase in the first half of 2022. The extent of COVID-19-related changes differed depending on region and pain unit size.ConclusionThe decreased number of hospital pain treatments during the pandemic implies a relevant analgesic undertreatment. During future pandemics, the ethical dimension of potentially non-sufficient pain treatment should be weighted against social, medical and hygienic restrictions influencing the hospitalization rate.
Identifying key predictors for uterine manipulator use in robotic simple hysterectomy: a retrospective cohort analysis
Authors: Kawamura Shogo , Ota Kuniaki , Ota Yoshiaki , Takahashi Toshifumi , Fujiwara Hitomi , Tasaka Keitaro , Okamoto Hana , Morimoto Yumiko , Saito Wataru , Sugihara Mika , Matsuyama Takehiko , Koike Eiji , Shiota Mitsuru , Shimoya Koichiro 
Published: September 2024

BackgroundRobotic simple hysterectomy (RSH) is the most common robotic gynecologic surgery in the United States. Uterine manipulators are commonly used to handle the uterus during laparoscopic surgery, but few studies have examined their necessity in RSH. This study retrospectively compares RSH cases with and without the use of manipulators, and identifies predictors for their intraoperative use.Materials and methodsThis retrospective cohort study included patients undergoing RSH for benign pathologies at Kawasaki Medical School from October 2020 to December 2022. Patients with malignancies were excluded. The robotic surgeries were performed by three skilled surgeons using the four-arm da Vinci Xi surgical system. Data on perioperative and operative parameters were collected, including age, body mass index (BMI), history of abdominal surgery, disease type, presence of ovarian cysts, and operative time. Statistical analyses were performed using EZR software, with multivariate logistic regression to identify predictive factors for uterine manipulator use.ResultsThe study included 113 patients who underwent RSH without a uterine manipulator and 58 with one. Patients without a manipulator were older, while those with a manipulator had higher BMIs and a higher prevalence of ovarian chocolate cysts and Douglas obliteration. Operating time was shorter without a manipulator. Independent predictors for manipulator use were higher BMI, presence of ovarian endometrioid cysts, and Douglas obliteration.ConclusionRSH without a uterine manipulator is feasible and can reduce the need for surgical assistants. Predictors for manipulator use include higher BMI, ovarian cysts, and Douglas obliteration. The use of a fourth robotic arm can enhance surgical independence and resource efficiency. Further research is needed to assess the long-term cost-effectiveness and outcomes of this approach.
In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts
Authors: Li Li , He Yuanyuan , Zou Qinghui , Chen Weiwei , Liu Yanxia , He Huifen , Zhang Jun 
Published: September 2024

BackgroundMalassezia furfur (M. furfur) is a prevalent dermatophyte that significantly impairs patients’ quality of life. This study aimed to evaluate the synergistic antifungal effects of combined extracts from Rosa rugosa Thunb. (MG) and Coptidis Rhizoma (HL) against M. furfur, both in vitro and in vivo.MethodsHigh-performance liquid chromatography (HPLC) was used to identify the major active compounds present in MG and HL. The antifungal activity of the combined Meilian extract (ML) was assessed using the checkerboard method and time-kill curves. Microstructural alterations in the fungi were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The impact of the extracts on the fungal cell membrane was investigated through propidium iodide staining, protein concentration assays, and ergosterol quantification. Transcriptomic analysis was conducted to elucidate the molecular mechanisms underlying the effects of the extracts. Furthermore, the synergistic antifungal effects of ML were evaluated in a mouse model of seborrheic dermatitis induced by M. furfur.ResultsThe study demonstrated that the combined application of MG and HL significantly affected the integrity of the M. furfur cell membrane and potentially modulated its formation processes. In the M. furfur-induced seborrheic dermatitis model, ML exhibited synergistic antifungal effects and effectively alleviated skin inflammation. These findings provide an important theoretical basis for understanding the antifungal mechanisms of ML and its potential application in dermatological therapy.