bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of hairpin loops and cruciforms across 118,065 genomes spanning the tree of life
Authors: Chantzi Nikol , Moeckel Camille , Chan Candace S. Y , Nayak Akshatha , Wang Guliang , Mouratidis Ioannis , Chartoumpekis Dionysios , Vasquez Karen M. , Georgakopoulos-Soares Ilias 
Published: September 2024

AbstractInverted repeats (IRs) can form alternative DNA secondary structures called hairpins and cruciforms, which have a multitude of functional roles and have been associated with genomic instability. However, their prevalence across diverse organismal genomes remains only partially understood. Here, we examine the prevalence of IRs across 118,065 complete organismal genomes. Our comprehensive analysis across taxonomic subdivisions reveals significant differences in the distribution, frequency, and biophysical properties of perfect IRs among these genomes. We identify a total of 29,589,132 perfect IRs and show a highly variable density across different organisms, with strikingly distinct patterns observed in Viruses, Bacteria, Archaea, and Eukaryota. We report IRs with perfect arms of extreme lengths, which can extend to hundreds of thousands of base pairs. Our findings demonstrate a strong correlation between IR density and genome size, revealing that Viruses and Bacteria possess the highest density, whereas Eukaryota and Archaea exhibit the lowest relative to their genome size. Additionally, the study reveals the enrichment of IRs at transcription start and termination end sites in prokaryotes and Viruses and underscores their potential roles in gene regulation and genome organization. Through a comprehensive overview of the distribution and characteristics of IRs in a wide array of organisms, this largest-scale analysis to date sheds light on the functional significance of inverted repeats, their contribution to genomic instability, and their evolutionary impact across the tree of life.
Glutamate Transport Proteins and Metabolic Enzymes are Poor Prognostic Factors in Invasive Lobular Carcinoma
Authors: Young Todd A. , Bahnassy Shaymaa , Abalum Theresa C. , Pope Eden A. , Rivera Amanda Torres , Fernandez Aileen I. , Olukoya Ayodeji O. , Mobin Dua , Ranjit Suman , Libbey Nicole E. , Persaud Sonali , Rozeboom Aaron M. , Chaldekas Krysta , Harris Brent T. , Madak-Erdogan Zeynep , Sottnik Joseph L. , Sikora Matthew J. , Riggins Rebecca B. 
Published: September 2024

AbstractInvasive Lobular Carcinoma (ILC) is a subtype of breast cancer characterized by distinct biological features, and limited glucose uptake coupled with increased reliance on amino acid and lipid metabolism. Our prior studies highlight the importance of glutamate as a key regulator of ILC tumor growth and therapeutic response. Here we examine the expression of four key proteins involved in glutamate transport and metabolism – SLC3A2, SLC7A11, GPX4, and GLUD1/2 – in a racially diverse cohort of 72 estrogen receptor-positive (ER+) ILC and 50 ER+ invasive ductal carcinoma, no special type (IDC/NST) patients with primary disease. All four proteins are associated with increased tumor size in ILC, but not IDC/NST, with SLC3A2 also specifically linked to shorter overall survival and the presence of comorbidities in ILC. Notably, GLUD1/2 expression is associated with ER expression in ILC, and is most strongly associated with increased tumor size and stage in Black women with ILC from our cohort and TCGA. We further explore the effects of GLUD1 inhibition in endocrine therapy-resistant ILC cells using the small-molecule inhibitor R162, which reduces ER protein levels, increases reactive oxygen species, and inhibits oxidative phosphorylation. These findings highlight a potentially important role for glutamate metabolism in ILC, particularly for Black women, and position several of these glutamate-handling proteins as potential targets for therapeutic intervention in ILC.
Unlocking the influence of psychosocial safety climate on work engagement and job satisfaction: exploring the mediating role of job demands
Authors: Gan Kia Hui , Kee Daisy Mui Hung 
Published: September 2024

Purpose
This study aims to investigate the influence of psychosocial safety climate (PSC) on work engagement, job satisfaction and the mediating role of job demands among young academics in Malaysian research universities.

Design/methodology/approach
The study participants consisted of 177 full-time lecturers employed by Malaysian research universities for at least one year. Respondents were selected using a purposive sampling technique.

Findings
The findings reveal the significance of PSC in fostering work engagement and job satisfaction by mitigating the impact of job demands. However, the finding indicates that the relationship between job demands and work engagement was not statistically significant.

Research limitations/implications
The necessity for a thorough three-wave research to precisely analyze the model’s longitudinal impacts was discovered in all correlation and cross-sectional studies.

Practical implications
The results indicate that the university should focus on job design and PSC support to enable university management to investigate further avenues for minimizing the impact of job demands to support academicians in RUs and enhance their job satisfaction. The results also indicated that young academicians’ work engagement might be improved by appropriate job demands.

Originality/value
To the best of the authors’ knowledge, this is the first study to examine these relationships among young academic staff in Malaysian universities. This paper offers recommendations to university policymakers on enhancing PSC, work engagement and job satisfaction among young academicians. It highlights the mediating role of job demands in Malaysian research universities.
Can logistics enterprises improve their competitiveness through ESG in the context of digitalization? Evidence from China
Authors: Fan Mingyue , Tang Yue , Qalati Sikandar Ali , Ibrahim Blend 
Published: September 2024

PurposeThis investigation endeavors to examine the routes by which environmental–social–governance (ESG) performance influences the competitive landscape for logistics enterprises, with a particular emphasis on the function of digitalization in this complex process. The research underscores the significance of the ESG context in the realm of digitalization, providing valuable insights into its impact on the overall competitiveness of logistics enterprises.Design/methodology/approachThis research gathers information from a total of 90 logistics enterprises that are publicly traded on the Shanghai and Shenzhen A-share stock markets for analysis and model testing. Due to the multiple pathways of influence and the constrained size of the sample, it has been decided that the Piecewise structural–equation–modeling (SEM) approach will be employed.FindingsThe research reveals that ESG factors positively impact enterprises' competitiveness (EC). The augmentation of competitiveness is attributed to the moderating role of green technology innovation (GTI) and agency costs between ESG and EC. In the context of digitalization, the level of digitalization of logistics enterprises may create a capital squeeze effect on environmental performance, weakening competitiveness. Conversely, the level of digitalization positively regulates the promoting effect of governance performance on competitiveness.Originality/valueThis research provides a sound theoretical foundation for understanding how ESG contributes to boosting the competitiveness of Chinese logistics enterprises and extends the application of Piecewise SEM in the research field of logistics enterprise competitiveness. Furthermore, it offers a practical pathway for companies to implement ESG practices and foster competitiveness in digital environments.
Cultivating resilience: Camburi Quilombo’s role in preserving local knowledge amid climate change
Authors: Carvalho Aline Vieira de , Campos Luana Cristina da Silva , Fachini Cristina , Silva João Paulo Soares , Otoni João Pedro 
Published: September 2024

PurposeThis paper examines how the Camburi Quilombo community in Ubatuba, Brazil, can be empowered to develop cultural resilience and conserve its Afro-Brazilian heritage in the face of challenges posed by climate change, urbanization and cultural erosion with the adverse consequences of tourism and inadequate infrastructure.Design/methodology/approachEmphasizing traditional knowledge, the project consisted of data collection, climate risk assessments and the development of an action plan in collaboration with academic institutions, governmental bodies and nongovernmental organizations (NGOs).FindingsAligned with sustainable development goals (SDGs), the project contributes to zero hunger, quality education, sustainable cities, climate action and life on land. Seed exchange fairs, decentralized seed banks and education initiatives foster environmental awareness and sustainable agriculture, impacting the community directly and over 1,000 individuals indirectly. While demonstrating short-term changes and mid-term viability, the project faced some challenges due to local communication complexities and overlapping jurisdiction.Originality/valueServing as a bridge between scientific research and local knowledge, the project aims to promote a paradigm shifts toward recognizing, respecting and incorporating indigenous practices, so that a more environmentally aware future can be envisioned for these communities amidst climate challenges. Reflecting on achievements, the project underscores integrating diverse cultural knowledge for community resilience.
Virtual reality hindrances in industrial training program: a Grey-DEMATEL analysis
Authors: Oliveira Carolina Pacheco de , Fettermann Diego Castro , Delgado Edwin Guillermo Castro , Moncayo Francisco Antonio 
Published: September 2024

Purpose
This study aims to analyze the relationship between users’ perceived barriers to using virtual and augmented reality technology from stakeholders, identifying perspectives and gaps regarding technology acceptance.

Design/methodology/approach
The Decision-Making Trial and Evaluation Laboratory method is useful for quantitatively evaluating the causal relationship between factors. Combining this method with the grey approach ensures greater accuracy even with limited information. A Scoping Review was conducted to identify the barriers, and data were extracted from a questionnaire administered to experts in the field.

Findings
Seven diverse barriers were identified by users, and cause-and-effect relationships were estimated. The results indicate three primary causal barriers and three highly dependent barriers. One of the identified barriers is not relevant in this model.

Originality/value
Virtual and augmented reality technologies have been gaining popularity since 2018, allowing users to interact with a virtual environment. These technologies are primarily used in training and education areas. However, due to the innovative nature of the topic, there are a small number of studies on technology acceptance, especially in the Brazilian context. Therefore, this paper contributes to the literature and assists in applying technologies in industries.
The influence of transformational and transactional leadership on employee retention: mediating role of employee engagement
Authors: Ali Mohammad , Niu Xiongying , Rubel Mohammad Rabiul Basher 
Published: September 2024

PurposeThe study aims to investigate the relationship between transformational, transactional leadership and employee retention with the mediating effect of employee engagement.Design/methodology/approachUsing judgemental sampling, data were collected from 650 employees serving at different levels in the pharmaceutical industry of Bangladesh. Structural equation modeling was employed to test the hypothesized relationships using AMOS.FindingsThe study found that transformational and transactional leadership significantly impact employee retention. The direct effects of transformational leadership (TFL) on employee engagement were found to be significant, while transactional leadership (TSL) has no significant direct influence on employee engagement. The output of the mediation analysis revealed that employee engagement significantly mediates the relationship between TFL and employee retention, whereas it showed an insignificant relationship between TSL and employee retention.Practical implicationsBased on the findings and the literature support, it can be postulated that an appropriate leadership style, especially TFL, that drives employee engagement can influence employees to stay with the organization longer. The study recommends that managers and leaders comprehend the importance of leadership and its appropriateness to retain valued employees by ensuring high workplace engagement.Originality/valueThe study would provide a unique insight into TFL and TSL practices and their impact on the pharmaceutical industry’s employee retention. This study also extends the research on employee engagement as a mediator between TFL and TSL and employee retention.
Utility of computed tomography brain scans in intubated patients with overdose
Authors: Lousick Michael , Edwards Serena , Keijzers Gerben , Pellatt Richard A F 
Published: September 2024

AbstractObjectiveDescribe the yield of computed tomography brain (CTB) scans in patients intubated for drug overdose.MethodsRetrospective descriptive study using existing registry data from two Australian EDs between February 2021 and June 2022.ResultsTwo hundred and six patients were intubated; 159 (77%) received a post‐intubation CTB scan. Of these, 38 out of 159 (24%) had a documented indication (i.e. head injury and seizure) for their scans, with a yield of 5.3% (95% confidence interval [CI] = 1.5–17%). Of the 121 out of 159 (76%) patients without an indication, the yield of CTB was 0% (95% CI = 0–3%).ConclusionsRoutine imaging of patients intubated for overdose without clinical indication is unjustified.
Streptococcus constellatus appendicitis in children: A case series
Authors: Shnaider Morya , Khalil Hadel , Zaitoon Hussein 
Published: September 2024

AbstractBackgroundStreptococcus constellatus (SC) is a pathogen well‐known for its involvement in pyogenic infections, with a notable preference for manifesting within the oral cavity, head and neck, and abdomen. This bacterium, belonging to the Streptococcus milleri group, has garnered attention for its propensity to incite inflammatory processes, especially in the abdominal region.ObjectivesThis study aims to present and characterise nine cases of paediatric patients with SC appendicitis.MethodsWe conducted a report on nine cases of SC appendicitis, all of whom presented to the emergency room with typical symptoms of appendicitis.ResultsPatients diagnosed with SC appendicitis exhibited a severe clinical course, necessitating appendectomy at presentation and requiring prolonged parenteral antimicrobial therapy. Furthermore, a higher readmission rate was observed among these patients, even several months after their initial discharge.ConclusionsSurgeons should be vigilant regarding SC appendicitis and its severe clinical course. Prolonged treatment with antimicrobial agents may be necessary for these patients to achieve better outcomes.
Harnessing the Potential of Living Labs in European Research Projects on Agriculture. The Case of Promoting Prudent Use of Antimicrobials in Livestock
Authors: Oehen Bernadette , Spaans Annick , Bonnet‐Beaugrand Florence , Fortané Nicolas , Kongsted Hanne , Vaarst Mette 
Published: September 2024

SummaryThe urgency to reduce the use of antimicrobials in the livestock sector has become part of the European Farm‐to‐Fork agenda. To develop innovations and pave the way for prudent antimicrobial use (AMU), the H2020 four‐year‐long ROADMAP project implemented the approach of Living Labs (LL). It stands for a process that involves a heterogeneous group of stakeholders in co‐creation, testing and evaluating innovations in real‐world contexts. The use of LLs has gained momentum to accelerate transformation of European agriculture by developing innovations through end‐user (farmer) involvement. However, the LL‐approach is new in the livestock sector. During the project, 11 LLs were established in seven European countries, each with its own settings but linked to the project's overarching goals. Some LLs developed a common understanding of AMU within the sector or between farmers and veterinarians, while others focussed on policy development or better management practices. During the ROADMAP project, we learned about the potential and limitations of the LL approach in the livestock sector. Using the approach of social innovations, we developed recommendations on how to realise the potential of LLs in a research context to meet the goals of the EU Farm‐to‐Fork strategy of prudent AMU.
Left to Live and Die: Resource Security and the Biopolitics of Land Stockpiling in China
Authors: Doll Ross 
Published: September 2024

AbstractBeginning in 2007, the Chinese state used liberalising policy and funding to encourage the expansion of large‐scale grain farming. Despite this support, many of the new farms have struggled financially and folded. Drawing on Foucauldian biopolitics and resource security literature, I argue that, with modernised agriculture, the state primarily sought to create not commercial farms, but the redundant farming infrastructure needed to buffer its growing reliance on food imports, abide by global trade regulations, and sustain its urban export manufacturing economy. These balancing efforts harmed commercial farmers: land commodification and policy funding incentivised urban government officials to intervene in rural land‐use planning, but low global market aligned grain prices disincentivised them from considering the place‐particular viability of their plans for producers. This article contributes to critical agrarian change literature by highlighting how modernist states become beholden to rational scientific techniques and sacrifice rural areas for increasingly vulnerable urban areas.
Plasma volume response patterns and a physiologic model of ultrafiltration in hemodialysis
Authors: Pietribiasi Mauro , Mitsides Nicos , Waniewski Jacek , Mitra Sandip 
Published: September 2024

AbstractBackgroundUltrafiltration (UF) is an essential process of restoring fluid homeostasis during hemodialysis (HD). Fluid shifts across the extracellular compartments during UF, predominantly across the capillary interface and between the macro‐ and microcirculation. A mismatch between UF and transcapillary fluid transport can lead to hemodynamic instability leading to cardiac morbidity. We wished to study intradialytic fluid transport characteristics and their variation during UF to identify factors that govern variability in transcapillary fluid movement in HD.MethodsTwenty‐two patients undergoing stable HD sessions were studied to measure and monitor absolute blood and plasma volume throughout UF. A computational mathematical model of predicted plasma volume decay during UF was analyzed with respect to the intradialytic real‐time data profile. Pre‐ and post‐dialysis fluid status was assessed using multifrequency bioimpedance spectroscopy. Serum electrolytes, osmolality, and total protein concentration were measured pre‐ and post‐dialysis and during the intradialytic phase.ResultsTwo distinct profiles of PV responses were detected. 60% of the patients presented plasma volume decline, characterized by a high percentage of volume decrease during the first hour, and a subsequent slower decrease with early rebound. The model was modified to achieve a proper fit of these volume profiles, assuming time‐dependent changes in selected parameters governing the refilling flow.ConclusionsAlthough the modified model could more accurately fit the data, the new parameter values often fell outside of a physiologically acceptable range, suggesting that other factors not included in the classic description of transcapillary fluid transport might be the cause of the observed patterns.
TMEM56 deficiency impairs the haem metabolism and cell cycle progression during human erythropoiesis
Authors: Wang Jingwei , Zhang Yingnan , Liu Jinhua , Wu Jing , Liang Yipeng , Xu Changlu , Ma Jinfa , Liang Jing , Zhao Yanhong , Zhang Xiaoru , Li Yue , Wang Di , Zheng Lingyue , Wang Ding , Jin Xu , Song Haoze , Zhu Xu , Cheng Qimei , Lin Lexuan , Gao Jie , Tong Jingyuan , Shi Lihong 
Published: September 2024

SummaryTMEM56, a gene coding a transmembrane protein, is abundantly expressed in erythroid cells. Despite this, its role in erythropoiesis has not been well characterized. In this study, we sought to clarify the function of TMEM56 in erythroid development, focusing specifically on its involvement in haem biosynthesis and cell cycle progression. To do this, we used CD34+ haematopoietic stem cells derived from umbilical cord blood and differentiated them into erythroid cells in an ex vivo model. Our results indicate that the loss of TMEM56 disrupts haem biosynthesis and impairs erythroid differentiation. Furthermore, deletion of Tmem56 in the erythroid lineage in murine models using erythropoietin receptor (EpoR)‐Cre revealed defects in erythroid progenitors within the bone marrow under both normal conditions and during haemolytic anaemia. These observations underscore the regulatory role of TMEM56 in maintaining erythroid lineage homeostasis. Taken together, our results unveil a previously unrecognized function of TMEM56 in erythroid differentiation and suggest its potential as an unfounded target for therapeutic strategies in the treatment of erythropoietic disorders.
Paediatric Laryngeal Ultrasound: A Retrospective Cohort Study in Aotearoa New Zealand and an International Survey
Authors: Marinone Lares Silvia Giovanna , Mackay Georgia , Clark Sita Tarini , Saniasiaya Jeyasakthy , McCaffer Craig 
Published: September 2024

ABSTRACTObjectiveVocal fold immobility (VFI) is a cause of significant morbidity and mortality in the paediatric population. Laryngoscopy is the current first‐line investigation for patients with suspected VFI. Laryngeal ultrasound (LUS) has recently emerged as an alternative method of identifying VFI. Compared to laryngoscopy, LUS is less invasive, does not require anaesthesia, and can be performed by non‐otolaryngologists. The objectives of this study are to evaluate LUS as a diagnostic method for the identification of VFI in a cohort of paediatric patients in Aotearoa New Zealand (NZ) and to estimate the frequency of use of LUS in the paediatric population by clinicians around the world.MethodsA retrospective, single‐centre cohort study was performed on all paediatric patients who had undergone laryngoscopy and LUS at Starship Children's Health in Auckland, NZ, between 2020 and 2023. An eight‐question survey was also developed and distributed to better understand clinicians' use of LUS in their clinical practice to diagnose paediatric VFI globally.ResultsTwenty‐nine paediatric patients met the inclusion criteria. LUS demonstrated good sensitivity (80.95%) for detecting VFI and increased to 93.33% for the detection of unilateral VFI. Of the 87 respondents to the survey, 41.38% utilise LUS in their clinical practice in the paediatric population. The main barriers to implementation of LUS as identified by non‐users were lack of equipment, expertise, and training.ConclusionsThese findings support the use of LUS as an accurate diagnostic tool for the detection of unilateral VFI. Further studies in non‐surgical populations and in patients with bilateral VFI, as well as standardised guidelines for LUS technique and reporting, are required.