bluuebunny/crossref_metadata_2025_split huggingface dataset:
RBM10: Harmful or helpful‐many factors to consider
Authors: Loiselle Julie J. , Sutherland Leslie C. 
Published: December 2017

AbstractRBM10 is an RNA binding motif (RBM) protein expressed in most, if not all, human and animal cells. Interest in RBM10 is rapidly increasing and its clinical importance is highlighted by its identification as the causative agent of TARP syndrome, a developmental condition that significantly impacts affected children. RBM10's cellular functions are beginning to be explored, with initial studies demonstrating a tumor suppressor role. Very recently, however, contradictory results have emerged, suggesting a tumor promoter role for RBM10. In this review, we describe the current state of knowledge on RBM10, and address this dichotomy in RBM10 function. Furthermore, we discuss what may be regulating RBM10 function, particularly the importance of RBM10 alternative splicing, and the relationship between RBM10 and its paralogue, RBM5. As RBM10‐related work is gaining momentum, it is critical that the various aspects of RBM10 molecular biology revealed by recent studies be considered moving forward. It is only if these recent advances in RBM10 structure and function are considered that a clearer insight into RBM10 function, and the disease states with which RBM10 mutation is associated, will be gained.
Study of the Enzymatic Activity of Arthrobacter ureafaciens Neuraminidase by Isothermal Titration Calorimetry
Authors: Chen Chien‐Sheng , Lee Wei‐Jen 
Published: December 2017

AbstractIsothermal titration calorimetry was used to determine the enzymatic activity and thermodynamic activation parameters of Arthrobacter ureafaciens sialidase with the sialyl substrates α‐2,3‐, α‐2,6‐sialyllactoses and α‐2,8‐sialic acid dimer. By monitoring the heat released during hydrolysis, the Michaelis constant (Km), catalytic rate constant (kcat), activation energy, activation Gibbs energy, enthalpy, and entropy for different monovalent sialyl conjugates were calculated and found to be consistent with those derived by chromatographic or colorimetric assays. The observed decreases in the activation energy and transition entropy of sialyllactoses were larger than the Michaelis activation parameters of lactose‐free di‐sialic acid because of the specific enzyme activity of A. ureafaciens sialidase.
A muscle‐specific MuRF1‐E2 network requires stabilization of MuRF1‐E2 complexes by telethonin, a newly identified substrate
Authors: Polge Cécile , Cabantous Stéphanie , Deval Christiane , Claustre Agnès , Hauvette Antoine , Bouchenot Catherine , Aniort Julien , Béchet Daniel , Combaret Lydie , Attaix Didier , Taillandier Daniel 
Published: December 2017

AbstractBackgroundMuscle wasting is observed in the course of many diseases and also during physiological conditions (disuse, ageing). Skeletal muscle mass is largely controlled by the ubiquitin‐proteasome system and thus by the ubiquitinating enzymes (E2s and E3s) that target substrates for subsequent degradation. MuRF1 is the only E3 ubiquitin ligase known to target contractile proteins (α‐actin, myosins) during catabolic situations. However, MuRF1 depends on E2 ubiquitin‐conjugating enzymes for ubiquitin chain formation on the substrates. MuRF1‐E2 couples are therefore putative targets for preventing muscle wasting.MethodsWe focused on 14 E2 enzymes that are either expressed in skeletal muscle or up‐regulated during atrophying conditions. In this work, we demonstrated that only highly sensitive and complementary interactomic approaches (surface plasmon resonance, yeast three‐hybrid, and split green fluorescent protein) allowed the identification of MuRF1 E2 partners.ResultsFive E2 enzymes physically interacted with MuRF1, namely, E2E1, E2G1, E2J1, E2J2, and E2L3. Moreover, we demonstrated that MuRF1‐E2E1 and MuRF1‐E2J1 interactions are facilitated by telethonin, a newly identified MuRF1 substrate. We next showed that the five identified E2s functionally interacted with MuRF1 since, in contrast to the non‐interacting E2D2, their co‐expression in HEK293T cells with MuRF1 led to increased telethonin degradation. Finally, we showed that telethonin governed the affinity between MuRF1 and E2E1 or E2J1.ConclusionsWe report here the first MuRF1‐E2s network, which may prove valuable for deciphering the precise mechanisms involved in the atrophying muscle programme and for proposing new therapeutical approaches.
The Role of Hydrophobicity in the Cellular Uptake of Negatively Charged Macromolecules
Authors: Abou Matar Tamara , Karam Pierre 
Published: December 2017

AbstractIt is generally accepted that positively charged molecules are the gold standard to by‐pass the negatively charged cell membrane. Here, it is shown that cellular uptake is also possible for polymers with negatively charged side chains and hydrophobic backbones. Specifically, poly[5‐methoxy‐2‐(3‐sulfopropoxy)‐1,4‐phenylenevinylene], a conjugated polyelectrolyte with sulfonate, as water‐soluble functional groups, is shown to accumulate in the intracellular region. When the polymer hydrophobic backbone is dissolved using polyvinylpyrrolidone, an amphiphilic macromolecule, the cellular uptake is dramatically reduced. The report sheds light on the fine balance between negatively charged side groups and the hydrophobicity of polymers to either enhance or reduce cellular uptake. As a result, these findings will have important ramifications on the future design of targeted cellular delivery nanocarriers for imaging and therapeutic applications.
Thermal and Lithographic Performance of Silsesquioxane with Cycloaliphatic Epoxy‐Siloxane Hybrid Spacer for Soft Lithography
Authors: Ramli Mohamad Riduwan , Ramli Rafiza , Mohamed Khairudin , Ahmad Zulkifli 
Published: December 2017

AbstractPattern replication and fidelity are crucial during soft lithography process. The flexibility and surface energy of the resist with that of the master mold are among the factors in determining such an effect. In this work, polysilsesquioxane bearing cycloaliphatic‐epoxy spacer of different chain length at tethered positions is synthesized. Pattern replication using soft lithography is made using polymethylmethacrylate (PMMA) as master mold. The thermal and UV‐cured lithographic performances are studied using differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). It shows that chain length of spacer induces flexibility and thermal stability. Despite chain flexibility, increase in spacer length results in poor lithographic performance. This can be attributed to the behavior of spreading parameter with different surface energy between the PMMA mold and the polysiloxane resist surfaces.
Review of Tools for Identifying, Planning, and Implementing Habitat Restoration for Pacific Salmon and Steelhead
Authors: Roni Philip , Anders Paul J. , Beechie Timothy J. , Kaplowe David J. 
Published: December 2017

Abstract
A key challenge in watershed restoration is identifying the appropriate assessments, data, and analyses needed to identify disrupted natural processes, lost and degraded habitats, and limiting factors to ultimately identify and design successful restoration projects. This has proven particularly challenging for large restoration programs focused on recovery of threatened and endangered salmon and trout where numerous tools, models, and other assessments have been developed to assist with habitat restoration at the watershed, reach, and project scale. Unfortunately, it is often unclear which step in the restoration process these various assessment tools will actually address. To assist with identifying the appropriate assessment tool (e.g., model, data collection, analysis, and survey), we reviewed major categories of watershed restoration assessment tools to determine their goals, inputs, outputs, and their utility in helping plan, prioritize, and implement restoration actions. The major categories of assessment tools reviewed were: (1) life cycle and fish–habitat models, (2) watershed assessment methods and techniques, (3) reach assessments, (4) prioritization tools, and (5) common monitoring methods to identify, prioritize, and plan river and watershed restoration projects. We specifically indicated whether these assessment tools directly or indirectly assisted with the key steps in the restoration process that are required to develop successful restoration plans and projects. These steps involve assessing watershed conditions, identifying limiting habitats and life stages, identifying problems and restoration actions, selecting restoration techniques, prioritizing restoration actions, or designing actual restoration projects. It is important to recognize that no single assessment tool will address all the steps in the restoration process. Selecting appropriate assessment tools requires a clear understanding of the goals of the restoration program and which step in the restoration process will be addressed by a particular tool. We provide recommendations for how restoration practitioners and managers can use our review to help select the appropriate assessment tools needed for their watershed.
Bio‐nanocomposites for food packaging applications: effect of cellulose nanofibers on morphological, mechanical, optical and barrier properties
Authors: Carvalho Raissa Alvarenga , Santos Taline Amorim , de Azevedo Viviane Machado , Felix Pedro Henrique Campelo , Dias Marali Vilela , Borges Soraia Vilela 
Published: December 2017

AbstractThe effect of the addition of various concentrations of cellulose nanofibers (CNF) on the thickness, solubility, morphology, mechanics, water vapor permeability and optical properties of biopolymers isolated from whey protein produced by the casting method was studied. The results show that the addition of CNF did not cause significant variation in the thickness of the films and resulted in nanocomposites with lower solubility and water vapor permeability. Scanning electron microscopy analysis showed that the films obtained with up to 4% of CNF showed good dispersion of the nanofibers in the whey protein matrix. The results of mechanical tests showed that the nanofibers acted as reinforcing material resulting in more resistant and less flexible films. © 2017 Society of Chemical Industry
Ring opening metathesis polymerization (ROMP) and thio‐bromo “click” chemistry approach toward the preparation of flame‐retardant polymers
Authors: Grubb Jonathan , Carosio Federico , Vasireddy Mallikharjun , Moncho Salvador , Brothers Edward N. , Hobbs Christopher E. 
Published: December 2017

ABSTRACTThis contribution describes our recent efforts geared toward the use of a general, thio‐bromo “click” reaction as a post‐polymerization method for the preparation of flame‐resistant polymeric materials. α‐bromo ester‐containing polymers could easily be prepared using ROMP and a subsequent, facile thio‐bromo click reaction was used for the installation of a phosphorus‐moiety that was shown to impart flame‐resistant/self‐extinguishing properties to these polymers. The extent of their flame resistance was then ascertained by treating paper (previously coated with polymer) to standard burn tests as well as measuring the limiting oxygen index (LOI). © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018, 56, 645–652
Linking animal movement and remote sensing – mapping resource suitability from a remote sensing perspective
Authors: Remelgado Ruben , Leutner Benjamin , Safi Kamran , Sonnenschein Ruth , Kuebert Carina , Wegmann Martin 
Published: December 2017

AbstractOptical remote sensing is an important tool in the study of animal behavior providing ecologists with the means to understand species–environment interactions in combination with animal movement data. However, differences in spatial and temporal resolution between movement and remote sensing data limit their direct assimilation. In this context, we built a data‐driven framework to map resource suitability that addresses these differences as well as the limitations of satellite imagery. It combines seasonal composites of multiyear surface reflectances and optimized presence and absence samples acquired with animal movement data within a cross‐validation modeling scheme. Moreover, it responds to dynamic, site‐specific environmental conditions making it applicable to contrasting landscapes. We tested this framework using five populations of White Storks (Ciconia ciconia) to model resource suitability related to foraging achieving accuracies from 0.40 to 0.94 for presences and 0.66 to 0.93 for absences. These results were influenced by the temporal composition of the seasonal reflectances indicated by the lower accuracies associated with higher day differences in relation to the target dates. Additionally, population differences in resource selection influenced our results marked by the negative relationship between the model accuracies and the variability of the surface reflectances associated with the presence samples. Our modeling approach spatially splits presences between training and validation. As a result, when these represent different and unique resources, we face a negative bias during validation. Despite these inaccuracies, our framework offers an important basis to analyze species–environment interactions. As it standardizes site‐dependent behavioral and environmental characteristics, it can be used in the comparison of intra‐ and interspecies environmental requirements and improves the analysis of resource selection along migratory paths. Moreover, due to its sensitivity to differences in resource selection, our approach can contribute toward a better understanding of species requirements.
Chlorofullerene C60Cl6: A Precursor for Straightforward Preparation of Highly Water‐Soluble Poly‐hydroxypyridinone Fullerene Derivatives as Potential Radionuclide Chelators
Authors: Zhang Qingchun , Jin Bo , Wang Xiaofang , Lei Shan , Liu Qiangqiang , Liang Hua , Chu Shijin , Peng Rufang 
Published: December 2017

AbstractRadionuclide internal contamination can induce chemical and radiological intoxication and produce harmful free radicals in vivo. Conventional studies on chelating agents have disregarded the damage of free radicals to the body. Therefore, novel poly‐hydroxypyridinone fullerene derivatives were designed and prepared by using chlorofullerene as precursor. The poor water solubility of fullerene was ameliorated by chemically modifying of hydrophilic hydroxypyridinone, and obtaining high Water‐soluble fullerene derivatives. The radical scavenging reaction kinetics of target products were studied. They all exhibited much higher rate constants than other fullerene polymer system. The CeIII ion was used as a simulated radionuclide for evaluating the binding ability of target products, and the values of conditional stability constants were about 5.15‐6.11. Thus, the bifunctional, Water‐soluble fullerene derivatives were successfully generated. The results provided basis for further study on the potential application of poly‐hydroxypyridinone fullerene derivatives as radionuclide chelators.
Immobilization of [Pd{(Ph2P)2N(CH2)3Si(OCH3)3‐κP,P'}X2] (X=Cl, Br) onto Montmorillonite: Investigating their Performance as Homogeneous or Heterogenized Suzuki‐Miyaura Catalysts
Authors: Stamatopoulos Ioannis , Roulia Maria , Vallianatou Kalliopi A. , Raptopoulou Catherine P. , Psycharis Vassilis , Carravetta Marina , Papachristodoulou Christina , Hey‐Hawkins Evamarie , Kostas Ioannis D. , Kyritsis Panayotis 
Published: December 2017

AbstractTwo palladium complexes, [Pd{(Ph2P)2N(CH2)3Si(OCH3)3‐κP,P'}X2], X=Cl (2), Br (3), were synthesized and characterized by spectroscopic methods and X‐ray crystallography. Complexes 2 and 3 contain a PdP2X2 square planar coordination sphere exhibiting small endocyclic P–Pd–P angles [71.49(4) and 71.53(5)°, respectively], the magnitude of which is controlled by the formation of the Pd–P–N–P four‐membered chelating ring. Complexes 2 and 3 were found to be efficient catalysts in the Suzuki‐Miyaura cross‐coupling reaction of aryl bromides with phenylboronic acid at ambient or elevated temperature. At 100 °C, using 0.1 mol% of palladium loading, high conversions were observed even after 15 min. Both complexes were immobilized onto STx‐1 montmorillonite clay via their −Si(OMe)3 moiety, a process investigated by X‐ray powder diffraction, X‐ray fluorescence spectrometry, solid‐state 31P NMR spectroscopy and Scanning Electron Microscopy. The immobilized complexes, as heterogenized catalysts, displayed higher activity in the Suzuki‐Miyaura coupling compared to that of the free complexes. The recyclability of the former was also explored.
Roles of p21, p53, cyclin D1, CDK‐4, estrogen receptor α in aflatoxin B1‐induced cytotoxicity in testicular tissue of mice
Authors: Zamir‐Nasta Toraj , Razi Mazdak , Shapour Hasanzadeh , Malekinejad Hassan 
Published: December 2017

AbstractThis study was done in order to investigate time‐dependent effect of AFB1 on expression of genes involving in cell cycle check point machinery at G, S, and M phases. For this purpose, 24 mature male Swiss albino mice were randomly divided into control and test groups. The animals in test group subdivided into three groups, which received the AFB1 at a daily dose of 20 µg/kg body weight, through intraperitoneal (i.p.) route, for 7, 14, and 21 days. The p21, p53, cyclin D1, CDK4, and ERα expressions at both mRNA and protein level were analyzed by using reverse transcription PCR (RT‐PCR) and immunohistochemistry, respectively. Moreover, the tubular differentiation (TDI) and spermiogenesis (SPI) indices were analyzed. Finally, the testicular DNA fragmentation was assessed by using DNA Ladder test. Observations revealed that the AFB1 remarkably (P < .05) reduced cyclin D1, Cdk4, and ERα expression at both mRNA and protein levels. Up‐regulated p21 and p53 expression was revealed in AFB1‐received animals, which developed time dependently. Histological examinations exhibited a significant reduction in TDI and SPI indices. Finally, the AFB1 resulted in severe DNA fragmentation. Our data showed that the AFB1 by down‐regulating the cyclin D1, Cdk4, and ERα expression adversely affects cyclin D1/Cdk4 and cyclin D1/ERα interactions. Moreover, the AFB1‐induced overexpression of p21 (as a kinase inhibitor), in turn results in cell cycle arrest via inhibiting the Cdk4 interaction with cyclin D1. Finally, the AFB1‐induced DNA damage triggers the p53‐dependent apoptosis pathway independent to p21 overexpression.
Sieve maximum likelihood estimation in generalized linear models with an unknown link function
Authors: Yuan Mengdie , Diao Guoqing 
Published: December 2017

Standard generalized linear models (GLMs) consist of three components: random component referring to a distribution of the response variable that belongs to the exponential family; systematic component referring to the linear predictor; and known link function specifying the relationship between the linear predictor and the mean of the distribution function. A flexible extension of the standard GLMs allows an unknown link function. Classical parametric likelihood approach is not applicable due to a large parameter space. To address this issue, sieve maximum likelihood estimation has been developed in literature in which the estimator of the unknown link function is assumed to lie in a sieve space. Various methods of sieves including the B‐spline and P‐spline based methods are introduced. The numerical implementation and theoretical properties of these methods are also discussed. WIREs Comput Stat 2018, 10:e1425. doi: 10.1002/wics.1425This article is categorized under:

Applications of Computational Statistics > Signal and Image Processing and Coding
Statistical and Graphical Methods of Data Analysis > Nonparametric Methods
Statistical Models > Generalized Linear Models
Algorithms and Computational Methods > Maximum Likelihood Methods
Mucosal Immunity: The Forgotten Arm of the Immune System
Authors: Wright Peter F , Ackerman Margaret E , Brickley Elizabeth B 
Published: December 2017

Abstract
The 2017 Stanley A. Plotkin Lecture in Vaccinology was delivered by Professor Peter F. Wright at the Pediatric Academic Societies Annual Meeting in San Francisco, California, in May 2017. The presentation provided an overview of the mucosal immune system as it applies to vaccinology. Specifically, Professor Wright’s lecture highlighted the remarkable opportunities for mucosal immunity research afforded by having both topically administered live vaccines and systemically administered inactivated vaccines available for the same pathogen. Using influenza and poliovirus case studies, Professor Wright described the use of live attenuated vaccines for human challenges and discussed how recent technological advancements in immunological assays have ushered in a new era for investigating the correlates of immune protection against wild-type infections at mucosal sites.
An Unusual Cluster of Neuroinvasive Lyme Disease Cases Presenting With Bannwarth Syndrome in the Midwest United States
Authors: Shah Aditya , O’Horo John C , Wilson John W , Granger Dane , Theel Elitza S 
Published: December 2017

Abstract
Bannwarth syndrome (BWS), an infrequent manifestation of neuroinvasive Lyme disease (LD) characterized by radiculopathy, neuropathy, and lymphocytic pleocytosis, is more commonly documented in Europe than North America. Here, we describe a cluster of 5 neuroinvasive LD cases with BWS in the upper Midwest United States between July and August 2017.
Moral Distress and Associated Factors Among Baccalaureate Nursing Students: A Multisite Descriptive Study
Authors: Krautscheid Lorretta , DeMeester Deborah A. , Orton Valorie , Smith Austin , Livingston Conor , McLennon Susan M. 
Published: December 2017

Abstract

AIM
The three study aims were to assess moral distress among senior baccalaureate nursing students, describe ethical dilemmas contributing to their moral distress in practice settings, and identify reasons for inaction when encountering dilemmas.

BACKGROUND
Previous studies have linked postlicensure nurses' moral distress to compassion fatigue, frustration, and turnover. Little is known about this phenomenon in students.

METHOD
This study employed a descriptive cross-sectional survey design to measure moral distress and reasons for not taking action across three academic sites (N = 267). Content analysis was used to identify themes of distressing clinical situations.

RESULTS
Aggregate mean moral distress rating was 3.12. Content analysis revealed compromised best practices, disrespect for human dignity, perceived constraints, and navigating personal values. The most frequent reasons for inaction were subordinate role, relationship preservation, incomplete knowledge, and uncertainty about speaking up.

CONCLUSION
Results help educators prioritize strategies to prevent and manage moral distress among students.
PhD Education Outcomes: Results of a National Survey of Nursing PhD Alumni
Authors: Ellenbecker Carol Hall , Nwosu Chizoba , Zhang Yuqing , Leveille Suzanne 
Published: December 2017

Abstract

AIM
A national survey was conducted to examine the relationship between individual students’ experiences and nursing PhD program characteristics and program outcomes.

BACKGROUND
In light of the shortage of doctorally prepared nurse scientists and a growth in nursing PhD programs, an examination of the PhD nursing education experience in relation to educational outcomes is timely.

METHOD
Data were collected from graduates of a 50 percent random sample of nursing PhD programs in the United States.

RESULTS
Graduates who had worked as research assistants, attended classroom-based programs, and worked as a nurse no more than 12 hours per week during their PhD education were more likely to have successful publication records and receive external research grant funding than graduates who did not have these experiences.

CONCLUSION
On the basis of traditionally accepted measures of research productivity, our results indicate that nursing PhD programs have limited success in producing nurse researchers.