bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway
Authors: Duan Hongwei , Wang Fang , Wang Ke , Yang Shuai , Zhang Rong , Xue Chen , Zhang Lihong , Ma Xiaofei , Du Xianghong , Kang Jian , Zhang Yong , Zhao Xingxu , Hu Junjie , Xiao Longfei 
Published: September 2024

Abstract
Background
Follicular cysts contribute significantly to reproductive loss in high-yield dairy cows. This results from the death of follicular granulosa cells (GCs) caused by oxidative stress. Quercetin is known to have significant antioxidant and anti-apoptotic effects. However, the effect of quercetin on follicular cysts has yet been elucidated. Therefore, this study aimed to explore the anti-oxidant and anti-apoptosis effects and potential molecular mechanisms of quercetin in H2O2-induced primary cow GCs and 3-nitropropionic acid (3-NPA)-induced mouse model of oxidative stress and thus treat ovarian cysts in dairy cows.

Results
In this study, compared with estrus cows, cows with follicular cysts showed heightened levels of oxidative stress and increased follicular cell apoptosis, while autophagy levels were reduced. A model of oxidative stress was induced in vitro by H2O2 and showed significant increases in apoptosis together with reduced autophagy. These effects were significantly ameliorated by quercetin. Effects similar to those of quercetin were observed after treatment of cells with the reactive oxygen species (ROS) inhibitor N-acetylcysteine (NAC). Further investigations using chloroquine (autophagy inhibitor), rapamycin (autophagy activator), selisistat (SIRT1 inhibitor), and compound C (AMPK inhibitor) showed that chloroquine counteracted the effects of quercetin on oxidative stress-induced apoptosis, while rapamycin had the same effect as quercetin. In addition, the SIRT1/AMPK pathway inhibitors antagonized quercetin-mediated mitigation of the effects of oxidative stress on increased apoptosis and reduced autophagy. Consistent with the results in vitro, in mouse ovarian oxidative stress model induced by 3-NPA, quercetin activated autophagy through the SIRT1/AMPK signaling pathway, while alleviating oxidative stress damage and inhibiting apoptosis in mouse ovaries.

Conclusions
These findings indicate that quercetin can inhibit apoptosis in GCs and restore ovarian function by activating autophagy through the SIRT1/ROS/AMPK signaling pathway, suggesting a new direction for the treatment of ovarian follicular cysts in high-yield dairy cows.
A case of resected anaplastic carcinoma of the pancreas producing granulocyte-colony stimulating factor with literature review
Authors: Kubo Norio , Suzuki Shigemasa , Seki Takahiro , Furuke Shunsaku , Yagi Naoki , Ooki Takashi , Aihara Ryusuke , Mogi Akira , Yoshida Yuka , Kashiwabara Kenji , Hosouchi Yasuo , Shirabe Ken 
Published: September 2024

Abstract
Background
Granulocyte colony-stimulating factor (G-CSF)-producing tumors have been reported in various organs, and the prognosis of patients with G-CSF-producing pancreatic cancers is particularly dismal. In this report, we present a case of G-CSF-producing anaplastic carcinoma of the pancreas (ACP), characterized by early postoperative recurrence and rapid, uncontrolled growth.

Case presentation
A 74-year-old man presented to our hospital with complaints of abdominal fullness and pain after eating. On admission, it was observed that the peripheral leukocyte counts and serum G-CSF levels were significantly elevated (23,770/µL and 251 pg/mL, respectively). Computed tomography of the abdomen revealed a pancreatic head tumor involving the superior mesenteric vein. Pathologically, ultrasound-guided fine-needle aspiration confirmed ACP. Subsequently, we performed a subtotal stomach-preserving pancreaticoduodenectomy with portal vein reconstruction and partial transverse colon resection. On postoperative day (POD) 7, the leukocyte count decreased from 21,180/μL to 8490/μL; moreover, computed tomography revealed liver metastasis. Therefore, mFOLFILINOX chemotherapy was initiated on POD 30. However, the tumor exhibited rapid progression, and the patient died on POD 45.

Conclusions
G-CSF-producing ACP is rare, and the prognosis of patients is extremely poor. Basic research is required to develop effective drugs against G-CSF-producing tumors, and large-scale studies using national databases are needed to develop multidisciplinary treatment methods.
Corporate (social) responsibility in state-business relations from the perspective of critical state theory: a historical case of early modern China
Authors: Kang Xu 
Published: September 2024

AbstractThis article presents a theoretical framework for analysing corporate responsibility through the lens of critical state theory, focusing on the dynamic interplay between state and business. Traditionally, corporate responsibility is seen as voluntary corporate actions for public welfare. However, it is increasingly viewed as integral to business strategy and subject to regulatory oversight. Utilizing Jessop’s strategic-relational approach, this study underscores the state’s role in structuring corporate behaviour, revealing how shifts in political elite networks influence corporate responsibility practices. A historical analysis of early modern China highlights these dynamics within specific national contexts characterized by developmentalism and authoritarianism, demonstrating the profound impact of state-business symbiosis on corporate responsibility. This analysis contributes to a deeper understanding of corporate responsibility in diverse political and economic settings, demonstrating how state power, corporate influence, and social welfare are mutually reinforcing and shaped by broader socio-political dynamics.
A comprehensive study of risk factors predicting hydrocephalus following decompressive craniectomy in traumatic brain injuries
Authors: Yadav Vikrant , Sahu Anurag , Pandey Nityanand , Prasad Ravi Shankar , Mishra Manish , Pradhan Ravi Shekhar 
Published: September 2024

Abstract
Introduction
Decompressive craniectomy [DC] is one of the leading armaments to lower refractory intracranial pressure. Post-DC hydrocephalus [PDCH] occurs in 11.9–36% of patients undergoing DCs for TBIs. Various theories have been given regarding pathophysiological mechanism of PDCH but remain dubious. Risk factors predicting PDCH still under research. Exact timeline regarding developmental process of PDCH remains undefined.

Method
This retrospective study was conducted on 422 patients who underwent DCs in our tertiary care trauma center over the period of one year. 60 patients out of 422 who developed PDCH were analyzed with respect to demographic variables and preoperative and postoperative risk factors. A total of 20 randomly selected patients, who underwent DCs but did not develop hydrocephalus, were selected and compared with patients who developed PDCH. Outcome analysis was done by dichotomizing the groups into independent and dependent groups.

Results
Among 422 patients undergoing DC, 14.21%[n = 60] developed PDCH. Younger [34.2 y vs 43.3 y, p = 0.0004] male age group was predominant in our study. Age [p = 0.021, multivariate analysis] and midline shift [p = 0.008, multivariate analysis] were significant preoperative predicting risk factors for PDCH. Interhemispheric hygroma [p = 0.031], brain bulge [ p = 0.008], and blood in postoperative scan [p = 0.029] were significant postoperative risk factors. Lower GCS score at admission [p = 0.0003], postoperative day 10 and at the time of establishment of PDCH were significantly predicted surgery to hydrocephalus time. Midline shift [p = 0.007] and thickness of interhemispheric hygroma [p = 0.021] were associated with poor outcome in patients with PDCH.

Conclusion
Younger age group and presence of midline shift are significant preoperative predictors of PDCH. Blood in postoperative scan, interhemispheric hygroma and brain bulge in postoperative period are significant predictors for PDCH. Deterioration in GCS score in postoperative period following DC should be taken as high index of suspicion for developing PDCH.
Prognostic models in multiple sclerosis: progress and challenges in clinical integration
Authors: Havla Joachim , Reeve Kelly , On Begum Irmak , Mansmann Ulrich , Held Ulrike 
Published: September 2024

AbstractAs a chronic inflammatory disease of the central nervous system, multiple sclerosis (MS) is of great individual health and socio-economic significance. To date, there is no prognostic model that is used in routine clinical care to predict the very heterogeneous course of the disease. Despite several research groups working on different prognostic models using traditional statistics, machine learning and/or artificial intelligence approaches, the use of published models in clinical decision making is limited because of poor model performance, lack of transferability and/or lack of validated models. To provide a systematic overview, we conducted a “Cochrane review” that assessed 75 published prediction models using relevant checklists (CHARMS, PROBAST, TRIPOD). We have summarized the relevant points from this analysis here so that the use of prognostic models for therapy decisions in clinical routine can be successful in the future.
Computer simulation and video media instructional packages in improving learning outcomes of Chemistry students in Ife Central Local Government Area
Authors: Fabeku Oluwatimilehin Emmanuel , Enyeasi Stanley Chizoba 
Published: September 2024

AbstractThe study examined computer simulation and video-media in improving students’ performance in Chemistry and compared students’ attitude towards Chemistry using the two packages. It also examined the effectiveness of the two packages in enhancing students’ retention of Chemistry concepts. This was with a view to finding out which of the two packages is better in enhancing students’ performance in Chemistry. The non-equivalent pretest—posttest control group research design was employed for the study. The population for the study comprised all Chemistry students in senior secondary schools in Osun State. Students in senior secondary class two (SSII) in their intact classes from three schools in Ife Central Local Government Area of the State formed the sample of the study. The three schools were randomly assigned to three groups; experimental group A using Computer Simulated Instructional Package (CSIP), experimental group B using Video Media Instructional Package (VMIP), and group C using the conventional teaching method. The instruments used for data collection were Chemistry Achievement Test (CAT) and Chemistry Attitudinal Questionnaire (CAQ). Data collected were analyzed using mean, ANOVA and ANCOVA statistics. The results revealed that there existed a significant difference in the effectiveness of computer simulation and video media instructional packages in improving students’ performance in Chemistry (F = 16.170, p < 0.05). This was further supported by the analysis of the post test scores of the respondents as those exposed to the computer simulation package had the highest post test score of 13.63 while conventional method had the lowest post test score of 9.46. The results also showed that there was no significant difference in the students’ attitude towards Chemistry using the two packages (F = 0.14, p > 0.05). Furthermore, the results showed that there existed a significant difference in the effectiveness of the two packages in enhancing students’ retention of Chemistry Concepts (F = 20.152, p < 0.05). Further analysis revealed a mean retention score of 14.79 for video media package, 15.54 for computer simulation package and 11.10 for the conventional method. This showed that those exposed to Computer simulation package had the highest average retention sore. The study concluded that computer simulation package is a more effective instructional package than video-media in improving students’ performance as well as retention of concepts taught. In terms of students’ attitude towards Chemistry, the two packages examined in the study suggest no effect and if such exists, is likely to be negligible.
Prevalence and CCR3-T51C genotype–phenotype correlation of bronchial asthma among basic education school children: an observational study
Authors: Zedan Magdy , Elwassefy Mona , Zareif Ismail El , ElTahan Hossam , Wahba Yahya 
Published: September 2024

Abstract
Background
Bronchial asthma (BA) is a chronic inflammatory disorder identified by different endotypes and phenotypes. Chemokine receptor 3 (CCR3) is one of the essential chemokine receptors that have a crucial role in asthma development by activating the migration of eosinophils through eotaxin production. We aimed to determine asthma prevalence among school children and to investigate the association between CCR3-T51C gene polymorphisms and the symptom-based clinical asthma phenotypes.

Methods
This study employed a hybrid design, conducted at a single center in Egypt from 2020 to 2021, to explore the relationship between asthma, its clinical phenotypes, and the CCR3-T51C gene polymorphism. Initially, a cross-sectional analysis was performed, utilizing a modified version of the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire to determine the prevalence of asthma in a cohort of 60 children, who presented with diverse clinical phenotypes, alongside 100 healthy controls. Subsequently, in the case–control phase of the study, we focused on examining the association between asthma (and its clinical phenotypes) and the CCR3-T51C gene polymorphism. For both groups, serum immunoglobulin E (IgE) levels and eosinophil counts were assessed, and the genotypes and alleles of the CCR3-T51C gene polymorphism were identified using the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) technique.

Results
A total of 600 children aged (6 to 16 years old) were enrolled. Out of these, 72 children (12%) were diagnosed with bronchial asthma in the basic education schools in El Manzala City, Egypt. Also, 72 (12%) of the studied children had wheezes, and 48 (8%) had night cough. Children with asthma had significantly higher relative eosinophil count and serum IgE levels than the control group. In terms of CCR3-T51C genotypes analysis, the TT genotype was the most prevalent in both patient and control groups, with 63.3% and 64%, respectively, but the difference was not statistically significant (P > 0.05). Also, there were no significant associations between CCR3-T51C genotypes and laboratory biomarkers among cough, wheezy, and cough and wheezy groups, except for the CT genotype in the cough group that had a lower eosinophil count than the wheezy group (P = 0.04).

Conclusion
Asthma affects 12% of the school-aged children. The CCR3-T51C genotype or allelic polymorphism frequency did not differ between asthmatics and controls; however, the TT genotype was more frequent in asthmatic children. Eosinophil count, serum IgE and gene polymorphism of CCR3-T51C appeared similar among different asthmatic phenotypes.
Competitive advantage and sustainability in Ghanaian microfinance institutions: the mediating role of strategic capabilities
Authors: Marfo Mensah , Ashitey ARMAH Abiel , Ofei Eleazer Fianko , Coffie Isaac Sewornu , Adadevoh Linda , Pattnaik Sanjeet Kumar , Reindoph Carl Asante , Annan Edward 
Published: September 2024

AbstractThis study explores the effect of competitive advantage on sustainability within Ghanaian microfinance institutions (MFIs), emphasizing the mediating role of strategic capabilities. We conducted a quantitative analysis using survey data from 500 managers representing 150 MFIs in Ghana, analyzed through structural equation modeling. Our findings indicate that competitive advantages and strategic capabilities have a positive significant influence on MFI sustainability. Additionally, strategic capabilities were found to mediate the relationship between competitive advantage and sustainability within Ghanaian MFIs. This study expands the theoretical framework by incorporating cost differentiation, pricing strategy, focus strategy, market strategy, efficient operations and risk management, innovation in product offerings, target market considerations, and client relationship management. We highlight how MFIs can maintain competitiveness through technology, partnerships, training, career development, and knowledge transfer. This mediated relationship underscores the crucial role of strategic capabilities in achieving sustainable operations. The study provides a conceptual framework for understanding MFI sustainability and offers a roadmap to enhance competitive advantage. By integrating elements from the competitive advantage theory and extending the resource-based view theory, this research contributes to the existing knowledge by clarifying the connections between competitive advantage and sustainability in the context of MFIs. Furthermore, the study addresses the dynamics of competitive advantage, strategic capabilities, and sustainability in a context with limited research. It offers practical implications for policymakers and practitioners, providing insights that can inform targeted interventions to enhance capacities and foster sustainability in the microfinance sector.
Giant enhancement of nonlinear harmonics of an optical-tweezer phonon laser
Authors: Xiao Guangzong , Kuang Tengfang , He Yutong , Chen Xinlin , Xiong Wei , Han Xiang , Tan Zhongqi , Luo Hui , Jing Hui 
Published: September 2024

AbstractPhonon lasers, as mechanical analogues of optical lasers, are unique tools for not only fundamental studies of the emerging field of phononics but also diverse applications such as deep-ocean monitoring, force sensing, and biomedical ultrasonics. Recently, nonlinear phonon-lasing effects were observed in an opto-levitated micro-sphere, i.e., the spontaneous emerging of weak signals of high-order phonon harmonics in the phonon lasing regime. However, both the strengths and the quality factors of the emerging phonon harmonics are very poor, thus severely hindering their potential applications in making and utilizing nonlinear phonon-laser devices. Here we show that, by applying a single-colour electronic injection to this levitated system, giant enhancement can be achieved for all higher-order phonon harmonics, with more than 3 orders enhanced brightness and 5 orders narrowed linewidth. Such an electronically-enhanced phonon laser is also far more stable, with frequency stability extended from a dozen of minutes to over 1 h. More importantly, higher-order phonon correlations, as an essential lasing feature, are confirmed to be enhanced by the electronic injection as well, which as far as we know, has not been reported in previous works using this technique. This work, providing much stronger and better-quality signals of coherent phonon harmonics, is a key step towards controlling and utilizing nonlinear phonon lasers for applications such as phonon frequency combs, broadband phonon sensors, and ultrasonic bio-medical diagnosis.
Molecular and biochemical components associated with chilling tolerance in tomato: comparison of different developmental stages
Authors: Camalle Maria Dolores , Levin Elena , David Sivan , Faigenboim Adi , Foolad Majid R. , Lers Amnon 
Published: September 2024

AbstractThe cultivated tomato, Solanum lycopersicum, is highly sensitive to cold stress (CS), resulting in significant losses during cultivation and postharvest fruit storage. Previously, we demonstrated the presence of substantial genetic variation in fruit chilling tolerance in a tomato recombinant inbred line (RIL) population derived from a cross between a chilling-sensitive tomato line and a chilling-tolerant accession of the wild species S. pimpinellifolium. Here, we investigated molecular and biochemical components associated with chilling tolerance in fruit and leaves, using contrasting groups of “chilling tolerant” and “chilling sensitive” RI lines. Transcriptomic analyses were conducted on fruit exposed to CS, and gene expressions and biochemical components were measured in fruit and leaves. The analyses revealed core responding genes specific to either the cold-tolerant or cold-sensitive RI lines, which were differentially regulated in similar fashion in both leaves and fruit within each group. These genes may be used as markers to determine tomato germplasm cold tolerance or sensitivity. This study demonstrated that tomato response to CS in different developmental stages, including seedling and postharvest fruit, might be mediated by common biological/genetic factors. Therefore, genetic selection for cold tolerance during early stages of plant development may lead to lines with greater postharvest fruit chilling tolerance.
Clinical Features and HLA Genetics Differ in Children at Type 1 Diabetes Onset by Hispanic Ethnicity
Authors: Karakus Kagan E , Fleury Theodore , Baschal Erin E , McDaniel Kristen A , Choi Hyelin , Armstrong Taylor K , Yu Liping , Simmons Kimber M , Michels Aaron W 
Published: September 2024

Abstract

Context
Type 1 diabetes incidence continues to increase in children, especially among Hispanic White (HW) children.

Objective
We investigated the clinical, immunologic, and genetic characteristics of HW and non-Hispanic White (NHW) children who presented at type 1 diabetes diagnosis.

Methods
In this single-center, observational study, children who were diagnosed with type 1 diabetes (≤20 years old) and tested for islet autoantibodies within 1 year of diagnosis were included in the study and divided into 2 groups by Hispanic ethnicity.

Results
Of 1297 children, 398 HW children presented with a younger age at diabetes onset (10.2 ± 3.9 vs 11.1 ± 4.1 years, P &lt; .001) and more diabetic ketoacidosis (62.4% vs 51.9%, P &lt; .001) than NHW children (n = 899). There was no difference in sex, A1c levels, or the number and prevalence of islet autoantibodies between the 2 cohorts. A subset of our cohort was human leukocyte antigen (HLA) typed as specific alleles confer strong genetic risk for type 1 diabetes (eg, HLA-DR4 and DQ8). Among 637 HLA-typed children, HW children had a significantly higher prevalence of the DR4-DQ8 haplotype than NHW children (79.1% vs 60.1%, P &lt; .001), and this frequency was much higher than a reference Hispanic population (OR 6.5, 95% CI 4.6-9.3).

Conclusion
Hispanic White children developing type 1 diabetes have a high prevalence of HLA DR4-DQ8, which can be utilized to select individuals for immune monitoring with islet autoantibodies to lessen diabetic ketoacidosis and potentially prevent diabetes onset.
When the Expected Becomes Unexpected: An Acute Presentation of Hypothyroidism
Authors: Martinez Sanchez Andrea , Vega Castellvi Claudia , Tarkoff Joshua , De La Vega Christie 
Published: September 2024

Abstract
Myxedema coma is an uncommon and life-threatening manifestation of severe hypothyroidism. Its occurrence in the pediatric population is exceptionally rare and can result from long-standing untreated hypothyroidism or nonadherence to treatment. Identifying this condition can be challenging because it requires a high level of clinical suspicion along with thyroid function testing. We present a 17-year-old female with a history of anxiety who had widespread nonspecific symptoms, including persistent bradycardia, which were found to be caused by hypothyroidism. Our goal is to raise awareness of the varied clinical manifestations of pediatric myxedema to promote early recognition and prompt medical interventions that can lead to better outcomes.