bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of ER stress, PI3K/AKT activation, and lung fibroblast proliferation in bleomycin-induced pulmonary fibrosis
Authors: Hsu Han-Shui , Liu Chen-Chi , Lin Jiun-Han , Hsu Tien-Wei , Hsu Jyuan-Wei , Su Kelly , Hung Shih-Chieh 
Published: October 2017

AbstractPulmonary fibrosis is characterized by fibroblast proliferation and extracellular matrix remodelling, leading to respiratory insufficiency. The mechanisms underlying this progressive and devastating disease remain unclear. Conditions that can impair the function of the endoplasmic reticulum (ER) cause accumulation of unfolded or misfolded proteins, resulting in ER stress and activation of the unfolded protein response (UPR). ER stress has been implicated in many conditions including cancer, diabetes, obesity, and inflammation. It is also involved in lung fibrosis, through myofibroblastic differentiation of fibroblasts; however, the precise role of ER stress in lung fibrosis is unknown. The current study aimed to investigate the underlying mechanisms of ER stress inhibitors in the treatment of bleomycin-induced lung fibrosis. We demonstrated that bleomycin can activate ER stress associated proteins, including GRP78, CHOP, and ATF-4, both in vitro and in vivo. PI3K/AKT acts upstream of ER stress to affect lung fibroblast proliferation, resulting in bleomycin-induced pulmonary fibrosis. Treatment with ER stress inhibitors or a PI3K inhibitor caused a reduction in fibroblast proliferation and improved pulmonary function. The relationship between PI3K/AKT/mTOR and ER stress in pulmonary fibrosis, and the application of PI3K inhibitors and ER stress inhibitors in the treatment of pulmonary fibrosis require further investigation.
Comprehensive single-cell transcriptome analysis reveals heterogeneity in endometrioid adenocarcinoma tissues
Authors: Hashimoto Shinichi , Tabuchi Yuta , Yurino Hideaki , Hirohashi Yoshihiko , Deshimaru Shungo , Asano Takuya , Mariya Tasuku , Oshima Kenshiro , Takamura Yuzuru , Ukita Yoshiaki , Ametani Akio , Kondo Naoto , Monma Norikazu , Takeda Tadayuki , Misu Sadahiko , Okayama Toshitugu , Ikeo Kazuho , Saito Tsuyoshi , Kaneko Shuich , Suzuki Yutaka , Hattori Masahira , Matsushima Kouji , Torigoe Toshihiko 
Published: October 2017

AbstractSingle cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo. Cancer cells in the E-side were highly malignant compared with those in the M-side. Many cells on the E-side were positive for spheroid-specific tumorigenesis-related markers including SOX2. In addition, there were higher numbers of epithelial-to-mesenchymal transition (EMT) cells in the E-side compared with the M-side. This study identified a site containing cells with high malignant potential such as EMT and cancer stem-like cells in cancer tissues. Finally, we demonstrate that established endometrioid adenocarcinoma subtype classifiers were variably expressed across individual cells within a tumor. Thus, such intratumoral heterogeneity may be related to prognostic implications.
Glycolipid-peptide conjugate vaccines enhance CD8+ T cell responses against human viral proteins
Authors: Speir M. , Authier-Hall A. , Brooks C. R. , Farrand K. J. , Compton B. J. , Anderson R. J. , Heiser A. , Osmond T. L. , Tang C. W. , Berzofsky J. A. , Terabe M. , Painter G. F. , Hermans I. F. , Weinkove R. 
Published: October 2017

AbstractAn important goal of vaccination against viruses and virus-driven cancers is to elicit cytotoxic CD8+T cells specific for virus-derived peptides. CD8+T cell responses can be enhanced by engaging help from natural killer T (NKT) cells. We have produced synthetic vaccines that induce strong peptide-specific CD8+T cell responsesin vivoby incorporating an NKT cell-activating glycolipid. Here we examine the effect of a glycolipid-peptide conjugate vaccine incorporating an NKT cell-activating glycolipid linked to an MHC class I-restricted peptide from a viral antigen in human peripheral blood mononuclear cells. The vaccine induces CD1d-dependent activation of human NKT cells following enzymatic cleavage, activates human dendritic cells in an NKT-cell dependent manner, and generates a pool of activated antigen-specific CD8+T cells with cytotoxic potential. Compared to unconjugated peptide, the vaccine upregulates expression of genes encoding interferon-γ, CD137 and granzyme B. A similar vaccine incorporating a peptide from the clinically-relevant human papilloma virus (HPV) 16 E7 oncoprotein induces cytotoxicity against peptide-expressing targetsin vivo, and elicits a better antitumor response in a model of E7-expressing lung cancer than its unconjugated components. Glycolipid-peptide conjugate vaccines may prove useful for the prevention or treatment of viral infections and tumors that express viral antigens.
Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus
Authors: Khan Samiullah , Roberts Juliet , Wu Shu-Biao 
Published: October 2017

AbstractTen reference genes were investigated for normalisation of candidate target gene expression data in the shell gland and spleen of laying hens challenged with two strains of infectious bronchitis virus (IBV). Data were analysed with geNorm, NormFinder and BestKeeper, and a comprehensive ranking (geomean) was calculated. In the combined data set of IBV challenged shell gland samples, the comprehensive ranking showed TATA-box binding protein (TBP) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) as the two most stable, and succinate dehydrogenase complex flavoprotein subunit A (SDHA) and albumin (ALB) as the two least stable reference genes. In the spleen, and in the combined data set of the shell gland and spleen, the two most stable and the two least stable reference genes were TBP and YWHAZ, and ribosomal protein L4 (RPL4) and ALB, respectively. Different ranking has been due to different algorithms. Validation studies showed that the use of the two most stable reference genes produced accurate and more robust gene expression data. The two most and least stable reference genes obtained in the study, were further used for candidate target gene expression data normalisation of the shell gland and spleen under an IBV infection model.
Flexible and Tunable 3D Gold Nanocups Platform as Plasmonic Biosensor for Specific Dual LSPR-SERS Immuno-Detection
Authors: Focsan M. , Craciun A. M. , Potara M. , Leordean C. , Vulpoi A. , Maniu D. , Astilean S. 
Published: October 2017

AbstractEarly medical diagnostic in nanomedicine requires the implementation of innovative nanosensors with highly sensitive, selective, and reliable biomarker detection abilities. In this paper, a dual Localized Surface Plasmon Resonance - Surface Enhanced Raman Scattering (LSPR- SERS) immunosensor based on a flexible three-dimensional (3D) gold (Au) nanocups platform has been implemented for the first time to operate as a relevant “proof-of-concept” for the specific detection of antigen-antibody binding events, using the human IgG - anti-human IgG recognition interaction as a model. Specifically, polydimethylsilane (PDMS) elastomer mold coated with a thin Au film employed for pattern replication of hexagonally close-packed monolayer of polystyrene nanospheres configuration has been employed as plasmonic nanoplatform to convey both SERS and LSPR readout signals, exhibiting both well-defined LSPR response and enhanced 3D electromagnetic field. Synergistic LSPR and SERS sensing use the same reproducible and large-area plasmonic nanoplatform providing complimentary information not only on the presence of anti-human IgG (by LSPR) but also to identify its specific molecular signature by SERS. The development of such smart flexible healthcare nanosensor platforms holds promise for mass production, opening thereby the doors for the next generation of portable point-of-care devices.
Ultrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations
Authors: Mestre Ana L. G. , Cerquido Mónica , Inácio Pedro M. C. , Asgarifar Sanaz , Lourenço Ana S. , Cristiano Maria L. S. , Aguiar Paulo , Medeiros Maria C. R. , Araújo Inês M. , Ventura João , Gomes Henrique L. 
Published: October 2017

AbstractUltra-sensitive electrodes for extracellular recordings were fabricated and electrically characterized. A signal detection limit defined by a noise level of 0.3–0.4 μV for a bandwidth of 12.5 Hz was achieved. To obtain this high sensitivity, large area (4 mm2) electrodes were used. The electrode surface is also micro-structured with an array of gold mushroom-like shapes to further enhance the active area. In comparison with a flat gold surface, the micro-structured surface increases the capacitance of the electrode/electrolyte interface by 54%. The electrode low impedance and low noise enable the detection of weak and low frequency quasi-periodic signals produced by astrocytes populations that thus far had remained inaccessible using conventional extracellular electrodes. Signals with 5 μV in amplitude and lasting for 5–10 s were measured, with a peak-to-peak signal-to-noise ratio of 16. The electrodes and the methodology developed here can be used as an ultrasensitive electrophysiological tool to reveal the synchronization dynamics of ultra-slow ionic signalling between non-electrogenic cells.
Combined x-ray crystallography and computational modeling approach to investigate the Hsp90 C-terminal peptide binding to FKBP51
Authors: Kumar Rajnish , Moche Martin , Winblad Bengt , Pavlov Pavel F. 
Published: October 2017

AbstractFK506 binding protein of 51 kDa (FKBP51) is a heat shock protein 90 (Hsp90) co-chaperone involved in the regulation of steroid hormone receptors activity. It is known for its role in various regulatory pathways implicated in mood and stress-related disorders, cancer, obesity, Alzheimer’s disease and corticosteroid resistant asthma. It consists of two FKBP12 like active peptidyl prolyl isomerase (PPIase) domains (an active FK1 and inactive FK2 domain) and one tetratricopeptide repeat (TPR) domain that mediates interaction with Hsp90 via its C-terminal MEEVD peptide. Here, we report a combined x-ray crystallography and molecular dynamics study to reveal the binding mechanism of Hsp90 MEEVD peptide to the TPR domain of FKBP51. The results demonstrated that the Hsp90 C-terminal peptide binds to the TPR domain of FKBP51 with the help of di-carboxylate clamp involving Lys272, Glu273, Lys352, Asn322, and Lys329 which are conserved throughout several di-carboxylate clamp TPR proteins. Interestingly, the results from molecular dynamics study are also in agreement to the complex structure where all the contacts between these two partners were consistent throughout the simulation period. In a nutshell, our findings provide new opportunity to engage this important protein-protein interaction target by small molecules designed by structure based drug design strategy.
Fault‐location method for HVDC transmission lines based on phase frequency characteristics
Authors: Li Congshan , He Ping 
Published: October 2017

A novel high‐voltage direct‐current (HVDC) line fault location method based on phase frequency change characteristics is proposed. The idea of the method is the travelling waves for the fault happened on different positions have different phase distortion characteristics, when they spread to the measuring terminal. By using the discrete Fourier analysis, the phase frequency characteristics were obtained. The frequency data of 40 to 60 Hz and the corresponding phase angle ratio data are selected, forming a N  × 3 matrix, each row of data represents the relationship between the fault location and phase angle ratio, fitting each row of data, respectively, the mathematical relationship between the fault location and phase angle ratio can be obtained, finally realise DC line fault location by seeking average. Finally, a lot of different faults are simulated and analysed on the PSCAD platform to verify the effectiveness and robustness of the proposed method.
Fast synthesis of methacrylated graphene oxide: a graphene‐functionalised nanostructure
Authors: Rahimi Mamaghani Kaveh , Naghib Seyed Morteza , Zahedi Alireza , Zeinali Kalkhoran Amir Hossein , Rahmanian Mehdi 
Published: October 2017

Graphene oxide (GO) was successfully synthesised via modified Hummer method and functionalised by methacrylate named MeGO (metacrylated GO). Different characterisation methods containing UV–vis, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, field emission scanning electron microscope (FESEM), transmission electron microscope (TEM) and atomic force microscope (AFM) analyses were employed to characterise the structure and morphology of MeGO. The outputs of the different analyses confirmed each other and proved the presence of the methacrylate functional group on the surface of the GO. Displacement in the peaks of UV–vis analysis coupled with the new peaks in FTIR results clearly demonstrated the creation of a new functional group. The G/D ratio of MeGO in Raman spectroscopy has increased to 1.17 and FESEM and TEM investigations confirmed the wrinkled structure of MeGO sheets. Also, it was observed that the distance between the GO layers was increased about 2.86 nm after functionalisation of GO layers by methacrylate groups. This nanomaterial with difference characterisation and specific properties in comparison with GO could be a great candidate for further developments in industrial and biomedical applications.
The 2016–2017 peak luminosity of the pre-main sequence variable V2492 Cygni
Authors: Giannini T. , Munari U. , Antoniucci S. , Lorenzetti D. , Arkharov A. A. , Dallaporta S. , Rossi A. , Traven G. 
Published: October 2017

Context. V2492 Cyg is a young pre-main sequence star presenting repetitive brightness variations of significant amplitude (ΔR ≥ 5 mag) whose physical origin has been ascribed to both extinction (UXor-type) and accretion (EXor-type) variability, although their mutual proportion has not been clarified yet. Recently, V2492 Cyg has reached a level of brightness ever registered in the period of its documented activity.Aim. We aim to derive the variation of the mass accretion rate between low- and high-state and to get new insights on the origin of the variability of V2492 Cyg.Methods. Optical and near-infrared (NIR) photometry and spectroscopy have been obtained in October 2016 and between March and July 2017. The source has remained bright until the end of May 2017, then it started to rapidly fade since the beginning of June at a rate of ~0.08 mag/day. On mid-July 2017 the source has reached the same low-brightness level as two years before. Extinction and mass accretion rate were derived by means of the luminosity of the brightest lines, in particular Hα and Hβ. A couple of optical high-resolution spectra are also presented to derive information on the gas kinematics.Results. Visual extinction variations do not exceed a few magnitudes, while the mass accretion rate is estimated to vary from less than 10−8 up to a few 10−7 M⊙ yr−1. This latter is comparable to that estimated on the previous high-state in 2010, likely occurred under more severe extinction conditions.Conclusions. The combined analysis of the optical and NIR observations extends to the present event the original suggestion that the V2492 Cyg variability is a combination of changing extinction and accretion.
Mutations in Coagulation Factor VIII Are Associated with More Favorable Outcome in Patients with Cutaneous Melanoma
Authors: Ping Zheng , Soni Abha , Williams Lance , Pham Huy , Basu Malay , Zheng X. Long 
Published: October 2017

AbstractCoagulation factor VIII (FVIII), von Willebrand factor (VWF), and ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats 13) play an important role in the regulation of normal hemostasis. However, little is known about their roles in patients with malignancy, particularly with cutaneous melanoma. Whole genome sequencing data are available for 25,719 cases in 126 cancer genomic studies for analysis. All sequencing data and corresponding pathology findings were obtained from The Cancer Genome Atlas. The cBioPortal bioinformatics tools were used for the data analysis. Our results demonstrated that mutations in genes encoding FVIII, VWF, and ADAMTS13 were reported in 92 of 126 cancer genomic studies, and high mutation rates in these three genes were observed in patients with cutaneous melanoma from three independent studies. Moreover, high mutation rates in FVIII, VWF, and ADAMTS13 were also found in patients with diffuse large B cell lymphoma (22.9%), lung small cell carcinoma (20.7%), and colon adenocarcinoma (19.4%). Among 366 melanoma cases from TCGA provisional, the somatic mutation rates of FVIII, VWF, and ADAMTS13 in tumor cells were 15, 14, and 5%, respectively. There was a strong tendency for coexisting mutations of FVIII, VWF, and ADAMTS13. Kaplan–Meier survival analysis demonstrated that melanoma patients with FVIII mutations had a more favorable overall survival rate than those without FVIII mutations (p = 0.02). These findings suggest, for the first time, that the FVIII mutation burden may have a prognostic value for patients with cutaneous melanoma. Further studies are warranted to delineate the molecular mechanisms underlying the favorable prognosis associated with FVIII mutations.
Diagnosis of Systemic Capillary Leak Syndrome in a Young Child Treated with Intravenous Immunoglobulin in the Acute Phase
Authors: Bjorklund Ashley , Fisher Gwenyth , Asmundsson Anna Sofi 
Published: October 2017

AbstractSystemic capillary leak syndrome (SCLS) is a potentially life-threatening disorder characterized by distributive shock, hypoalbuminemia, and hemoconcentration. It is exceedingly rare in children with less than 20 cases reported to date. The underlying cause for this syndrome remains largely unknown and acute treatment has remained mainly supportive. Prophylaxis with intravenous immunoglobulin (IVIG) has been shown to successfully prevent further episodes in both adults and children. We present a case of a 2-year-old previously healthy male admitted to the pediatric intensive care unit with a clinical course consistent with SCLS. His shock was refractory to aggressive fluid and vasopressor support. Reversal of SCLS with IVIG given in the acute phase had been described in three adult subjects, and for this reason, the decision was made to administer IVIG. Within an hour of administration, hemodynamics stabilized and vasopressor support could be weaned. He has had no further episodes on prophylactic infusions of IVIG. Although the exact mechanism of IVIG in SCLS is unknown, it has proven to be an effective and safe prophylactic therapy, and in our patient, it drastically reversed the acute capillary leak. We suggest that IVIG should be considered as acute therapy in pediatric patients with refractory shock and a clinical course suggestive of SCLS.
Embryonic Tumor with Abundant Neuroepithelium and True Rosettes (ETANTR): Case Report and Literature Review
Authors: Tavares Cleciton , Gomes-Braga Francisca , Sousa Emerson , Ferreira Camilla , Brito José 
Published: October 2017

AbstractThe embryonal tumor with abundant neuropil and true rosettes (ETANTR) is an extremely rare variant of the primitive neuroectodermal tumor (PNET). About 80 cases have been reported since its first description in the literature, in 2000. The ETANTR occurs in very young patients, especially children under 6 years of age. It is found mainly in the cerebral cortex. Headache, focal neurological signs, seizures, increased head circumference and psychomotor developmental delay are the most frequent symptoms of ETANTR. Histologically, it displays the features of an ependymoblastoma and a neuroblastoma, showing areas of neuroepithelium fibrillar rosettes with ependymoblastic zones and interposed undifferentiated neuroepithelial cells. The ETANTR is distinguishable from other embryonal tumors due to the abundance of neuroepithelium. Genetic studies have demonstrated the presence of polysomy of chromosome 2 and chromosome 19q13.42 amplification. This is an extremely aggressive tumor with a mean survival ranging from 9 to 48 months. We present the first report in Brazil, published in indexed literature, of an ETANTR case involving a young child.
Magen-OP zur Behandlung der Fettsucht
Authors: Thalheimer Andreas 
Published: October 2017

Zusammenfassung
Operationsverfahren Für Patienten, die unter krankhaftem Übergewicht
leiden, ist die Adipositas-Chirurgie eine wirksame Option, um Gewicht zu
verlieren und metabolische Begleiterkrankungen zu reduzieren. Wir stellen Ihnen
die laparoskopische Magenbypass-Operation und die laparoskopische
Schlauchmagen-Operation vor.
Langsam essen und genießen
Authors: Peters Jutta 
Published: October 2017

Zusammenfassung
Ernährungsmedizin bei morbider Adipositas Patienten mit einem
adipositaschirurgischen Eingriff müssen ihre Ernährungsgewohnheiten komplett
umstellen. Welche Konzepte besonders gut geeignet sind, um die Energiezufuhr zu
verringern und eine ausgeglichene Nährstoffversorgung zu gewährleiten,
beschreibt unsere Autorin.
Endogene Verbrennungen im OP
Authors: Weimer Tobias , Jaeger Laura 
Published: October 2017

Zusammenfassung
Recht Operationen bieten mannigfaltige Risiken. Wie ist es aber um die
Handhabung und die Gefahren der Elektrochirurgie im Operationssaal bestellt?
Existieren einschlägige Urteile im Zusammenhang mit Verbrennungen im
Operationssaal? Wer ist verantwortlich und gelten Verbrennungen eigentlich als
vermeidbare Ereignisse? Unser Fachanwalt gibt Antworten.
Tropische Infektionserkrankungen bei schwangeren Migrantinnen in Deutschland
Authors: Schleenvoigt Benjamin , Richter Joachim , von Weizsäcker Katharina , Mathias W. , Jablonka Alexandra 
Published: October 2017

ZusammenfassungIm Jahr 2016 wurden in Deutschland mehr als 700 000 Asylerstanträge gestellt. Das häufigste Herkunftsland war Syrien. Der Anteil weiblicher Migranten lag in verschiedenen Betrachtungen zwischen 20 und 35 %. Mit schwangeren Migrantinnen muss in 3,5 % gerechnet werden. In dieser vulnerablen Patientengruppe sollte gezielt nach Risikofaktoren für einen ungünstigen Verlauf der Schwangerschaft gesucht werden. Im Hinblick auf das infektiologische, tropenmedizinische Erregerspektrum sind dies vor allem die Tuberkulose und parasitäre Erkrankungen. Bei Afrikanern sind Bilharziose und Malaria von hoher Relevanz. Bei Lateinamerikanern steht die Chagas-Erkrankung im Vordergrund. Aktuelle Studien zum Erkrankungsspektrum von Migrantinnen, die ein Kind erwarten, stehen bisher nicht zur Verfügung.
Vorbereitung für Trekking und Höhenbergsteigen
Authors: Burtscher Martin 
Published: October 2017

ZusammenfassungBergsport erfreut sich weltweit zunehmender Beliebtheit und gerade größere Höhen scheinen eine besondere Faszination auszuüben. Etwa 10 % jener Personen, die sich akut und ohne Vorakklimatisation in Höhenlagen von etwa 2500 m begeben, leiden unter akuter Bergkrankheit. Dieser Prozentsatz erhöht sich auf 30–40 % bei Anstiegen auf etwa 3500 m und über 50 % in Höhenlagen über 4000 m. In seltenen Fällen können Höhenerkrankungen tödlich verlaufen (Höhenhirnödem und Höhenlungenödem). Die wichtigste Präventionsmaßnahme stellt der langsame und individuell dosierte Anstieg dar. Zusätzlich können Strategien der Vorakklimatisation in den heimischen Bergen und der Einsatz von simulierten Höhenexpositionen (Höhenzelt, -räume) wertvolle Akklimatisationsunterstützung darstellen.