bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo
Authors: Chowdhary Sayali , Tomer Darshika , Dubal Dnyanesh , Sambre Devashree , Rikhy Richa 
Published: July 2017

AbstractMitochondria are inherited maternally as globular and immature organelles in metazoan embryos. We have used the Drosophila blastoderm embryo to characterize their morphology, distribution and functions in embryogenesis. We find that mitochondria are relatively small, dispersed and distinctly distributed along the apico-basal axis in proximity to microtubules by motor protein transport. Live imaging, photobleaching and photoactivation analyses of mitochondrially targeted GFP show that they are mobile in the apico-basal axis along microtubules and are immobile in the lateral plane thereby associating with one syncytial cell. Photoactivated mitochondria distribute equally to daughter cells across the division cycles. ATP depletion by pharmacological and genetic inhibition of the mitochondrial electron transport chain (ETC) activates AMPK and decreases syncytial metaphase furrow extension. In summary, we show that small and dispersed mitochondria of the Drosophila blastoderm embryo localize by microtubule transport and provide ATP locally for the fast syncytial division cycles. Our study opens the possibility of use of Drosophila embryogenesis as a model system to study the impact of maternal mutations in mitochondrial morphology and metabolism on embryo patterning and differentiation.
Morphogenesis and Optoelectronic Properties of Supramolecular Assemblies of Chiral Perylene Diimides in a Binary Solvent System
Authors: Shang Xiaobo , Song Inho , Ohtsu Hiroyoshi , Tong Jiaqi , Zhang Haoke , Oh Joon Hak 
Published: July 2017

AbstractChiral supramolecular structures are attracting great attention due to their specific properties and high potential in chiral sensing and separation. Herein, supramolecular assembling behaviors of chiral perylene diimides have been systematically investigated in a mixed solution of tetrahydrofuran and water. They exhibit remarkably different morphologies and chiral aggregation behaviors depending on the mixing ratio of the solvents, i.e., the fraction of water. The morphogenesis and optoelectronic properties of chiral supramolecular structures have been thoroughly studied using a range of experimental and theoretical methods to investigate the morphological effects of chiral supramolecular assemblies on the electrical performances and photogenerated charge-carrier behaviors. In addition, chiral perylene diimides have been discriminated by combining vibrational circular dichroism with theoretical calculations, for the first time. The chiral supramolecular nanostructures developed herein strongly absorb visible spectral region and exhibit high photoresponsivity and detectivity, opening up new opportunities for practical applications in optoelectronics.
Netrin-1 promotes glioma growth by activating NF-κB via UNC5A
Authors: Chen Jing-Ying , He Xiao-Xiao , Ma Chi , Wu Xin-Min , Wan Xi-Lin , Xing Zhen-Kai , Pei Qing-Qing , Dong Xian-Ping , Liu Dong-Xu , Xiong Wen-Cheng , Zhu Xiao-Juan 
Published: July 2017

AbstractGliomas, a common type of brain tumor, are characterized by aggressive infiltration, making it difficultly to cure by surgery. Netrin-1, an extracellular guidance cue critical for neuronal axon path-finding, has been reported to play an important role in cell invasion and migration in several types of cancers. However, the role of netrin-1 in glioma remains largely unknown. Here, we provide evidence suggested that Netrin-1 has a critical role in glioma growth. We found that netrin-1 was significantly increased in glioma samples and positively correlated with cell proliferation, tumor grade and malignancy. Netrin-1 knockdown reduced cell proliferation and attenuated tumor growth in a xenograft mouse model. Further studies found that netrin-1 induced NF-κB p65ser536 phosphorylation and c-Myc expression in vitro and in vivo. Interestingly, activation of NF-κB by netrin-1 was dependent on UNC5A receptor, because suppression of UNC5A significantly inhibited NF-κB p65ser536 phosphorylation, c-Myc up-regulation and reduced cell proliferation. Taken together, these results suggested netrin-1 promotes glioma cell proliferation by activating NF-κB signaling via UNC5A, netrin-1 may be a potential therapeutic target for the treatment of glioma.
Interface Adhesion and Structural Characterization of Rolled-up GaAs/In0.2Ga0.8As Multilayer Tubes by Coherent Phonon Spectroscopy
Authors: Brick D. , Engemaier V. , Guo Y. , Grossmann M. , Li G. , Grimm D. , Schmidt O. G. , Schubert M. , Gusev V. E. , Hettich M. , Dekorsy T. 
Published: July 2017

AbstractWe present a detailed experimental and theoretical study of the acoustic phonon modes in rolled-up multilayers with thickness of the layers in the nanometre and diameters in the micrometre range. We compare our results to planar, unrolled multilayers grown by molecular beam epitaxy. For the planar multilayers the experimentally obtained acoustic modes exhibit properties of a superlattice and match well to calculations obtained by the Rytov model. The rolled-up superlattice tubes show intriguing differences compared to the planar structures which can be attributed to the imperfect adhesion between individual tube windings. A transfer matrix method including a massless spring accounting for the imperfect adhesion between the layers yields good agreement between experiment and calculations for up to five windings. Areas with sufficient mechanical coupling between all windings can be distinguished by their acoustic mode spectrum from areas where individual windings are only partially in contact. This allows the spatially resolved characterization of individual tubes with micrometre spatial resolution where areas with varying interface adhesion can be identified.
Facile fabrication of superparamagnetic graphene/polyaniline/Fe3O4 nanocomposites for fast magnetic separation and efficient removal of dye
Authors: Mu Bin , Tang Jie , Zhang Long , Wang Aiqin 
Published: July 2017

AbstractUsing graphene as adsorbent for removal of pollutants from polluted water is commonly recognized to be costly because the graphene is usually produced by a very complex process. Herein, a simple and eco-friendly method was employed to fabricate efficient superparamagnetic graphene/polyaniline/Fe3O4 nanocomposites for removal of dyes. The exfoliation of graphite as nanosheets and the functionalization of nanosheets with polyaniline and Fe3O4 nanoparticles were simultaneously achieved via a one-pot reaction process combining the intercalation polymerization of aniline and the co-precipitation of the residual Fe3+ and the generated Fe2+. The obtained graphene/polyaniline/Fe3O4 nanocomposites exhibited excellent adsorption performance for Congo red, even in the presence of Brilliant green. The adsorption kinetics and adsorption isotherms were well fitted with pseudo second-order kinetic model and Langmuir isotherm model, respectively. In a word, this method is simple and industrially feasible, which provides a new approach to fabricate highly efficient graphene-based adsorbents on large scale for removal of dyes. In addition, it also can be used to exfoliate other two-dimensional materials, such as boron nitride, carbon nitride and MoS2 for a range of possible applications.
The dental phenotype of hairless dogs with FOXI3 haploinsufficiency
Authors: Kupczik Kornelius , Cagan Alexander , Brauer Silke , Fischer Martin S. 
Published: July 2017

AbstractHairless dog breeds show a form of ectodermal dysplasia characterised by a lack of hair and abnormal tooth morphology. This has been attributed to a semi-dominant 7-base-pair duplication in the first exon of the forkhead box I3 gene (FOXI3) shared by all three breeds. Here, we identified this FOXI3 variant in a historical museum sample of pedigreed hairless dog skulls by using ancient DNA extraction and present the associated dental phenotype. Unlike in the coated wild type dogs, the hairless dogs were characterised in both the mandibular and maxillary dentition by a loss of the permanent canines, premolars and to some extent incisors. In addition, the deciduous fourth premolars and permanent first and second molars consistently lacked the distal and lingual cusps; this resulted in only a single enlarged cusp in the basin-like heel (talonid in lower molars, talon in upper molars). This molar phenotype is also found among several living and fossil carnivorans and the extinct order Creodonta in which it is associated with hypercarnivory. We therefore suggest that FOXI3 may generally be involved in dental (cusp) development within and across mammalian lineages including the hominids which are known to exhibit marked variability in the presence of lingual cusps.
Altered Brain Network Connectivity as a Potential Endophenotype of Schizophrenia
Authors: Li Peng , Fan Teng-Teng , Zhao Rong-Jiang , Han Ying , Shi Le , Sun Hong-Qiang , Chen Si-Jing , Shi Jie , Lin Xiao , Lu Lin 
Published: July 2017

AbstractAbnormal functional brain connectivity could be considered an endophenotype of psychosis in schizophrenia. Identifying candidate endophenotypes may serve as a tool for elucidating its biological and neural mechanisms. The present study investigated the similarities and differences of features of brain network connectivity between patients and their first-degree relatives. Independent component analysis was conducted on imaging data collected from 34 healthy controls, 33 schizophrenia patients, and 30 unaffected first-degree relatives. The correlation between functional connectivity with neurocognitive performance and clinical symptoms were calculated. Abnormalities of between-network connectivity largely overlapped in patients and first-degree relatives, but the extent of such abnormalities was relatively minor in relatives. Negative connectivity between language networks and executive control networks was impaired in schizophrenia patients and their first-degree relatives, and this decreased connectivity was correlated with performance in language processing. Similar impairments were found in high-visual network and executive network coupling, and this decreased connection was correlated with the severity of positive symptoms in patients. The results indicated that abnormal functional connectivity within and between perceptual systems (i.e., high-visual and language) and executive control networks was related to the generic risk of schizophrenia, which makes it a potential endophenotype for schizophrenia.
Biological analysis of cancer specific microRNAs on function modeling in osteosarcoma
Authors: Wang Hao , Tang Min , Ou Liping , Hou Mengyi , Feng Tianyu , Huang Yu-E , Jin Yaqian , Zhang Heng , Zuo Guowei 
Published: July 2017

AbstractOsteosarcoma (OS) is the most common bone tumor characterized with a high risk of amputation and malignant morbidity among teenagers and adolescents. However, relevant pathogenic/biological mechanisms underlying OS-genesis remains to be ambiguous. The aim of this study was to elucidate functional relationship about microRNAs-mRNAs networks and to identify potential molecular markers via a computational method. Gene expression profile (GSE70415) was recruited from Gene Expression Omnibus. 3856 differentially expressed genes and 250 significantly expressed microRNAs were identified by using GCBI. The results of GO and KEGG pathways associated proteomics analysis indicated that extracellular matrix organization, small molecule metabolic process, cell adhesion (GO IDs: 0030198, 0044281, 0007155) and pathways in cancer, PI3K-Akt signaling pathway, metabolic pathways (pathway IDs: 5200, 4151, 1100) were significantly enriched. In addition, CKMT2, miR-93b-5p, miR-29b-3p were found to be positively/negatively correlated with TP53, EGFR, and MMP members mediated OS development, including angiogenesis, migration and invasion. Further visualization of collective effect of 1181 microRNAs-mRNAs pairs and protein-protein interactions was realized by applying with cytosacpe. In summary, our work provided a better understanding of non-coding regulatory mechanisms of transcriptomics and unraveled essential molecular biomarkers in osteosarcoma.
Deep Learning based Radiomics (DLR) and its usage in noninvasive IDH1 prediction for low grade glioma
Authors: Li Zeju , Wang Yuanyuan , Yu Jinhua , Guo Yi , Cao Wei 
Published: July 2017

AbstractDeep learning-based radiomics (DLR) was developed to extract deep information from multiple modalities of magnetic resonance (MR) images. The performance of DLR for predicting the mutation status of isocitrate dehydrogenase 1 (IDH1) was validated in a dataset of 151 patients with low-grade glioma. A modified convolutional neural network (CNN) structure with 6 convolutional layers and a fully connected layer with 4096 neurons was used to segment tumors. Instead of calculating image features from segmented images, as typically performed for normal radiomics approaches, image features were obtained by normalizing the information of the last convolutional layers of the CNN. Fisher vector was used to encode the CNN features from image slices of different sizes. High-throughput features with dimensionality greater than 1.6*104 were obtained from the CNN. Paired t-tests and F-scores were used to select CNN features that were able to discriminate IDH1. With the same dataset, the area under the operating characteristic curve (AUC) of the normal radiomics method was 86% for IDH1 estimation, whereas for DLR the AUC was 92%. The AUC of IDH1 estimation was further improved to 95% using DLR based on multiple-modality MR images. DLR could be a powerful way to extract deep information from medical images.
Alternative functions of Hd1 in repressing or promoting heading are determined by Ghd7 status under long-day conditions
Authors: Zhang Zhanyi , Hu Wei , Shen Guojing , Liu Haiyang , Hu Yong , Zhou Xiangchun , Liu Touming , Xing Yongzhong 
Published: July 2017

AbstractPrevious studies suggested thatHd1promoted heading under short-day conditions (SD) and delayed heading under long-day conditions (LD). However in this study,Hd1was demonstrated to consistently promote heading date in Zhenshan 97 (ZS97) background by upregulatingEhd1,Hd3aandRFT1expression under both SD and LD. While the high photoperiod sensitivity ofHd1was observed in Minghui 63 (MH63) background, with heading being suppressed in LD but promoted in SD. Comparative analysis of two sets of near isogenic lines ofHd1in MH63 and ZS97 backgrounds indicated that the alternative functions ofHd1in promoting or suppressing heading under LD are dependent on the previously cloned flowering repressor geneGhd7. The interaction between proteins Ghd7 and Hd1 occurred through binding of the CCT domain of Ghd7 to the transcription-activating domain of Hd1, resulting in suppression ofEhd1and florigen gene expression. The involvement of the transcription-activating domain of Hd1 in this protein-protein interaction probably blocked or weakened its transcriptional activity. These findings suggest thatHd1alone essentially acts as a promoter of heading date, and the protein interaction between Ghd7 and Hd1 determines photoperiod sensitivity and integratedHd1-mediated andEhd1-mediated flowering pathways in rice.
Triazophos (TAP) removal in horizontal subsurface flow constructed wetlands (HSCWs) and its accumulation in plants and substrates
Authors: Wu Juan , Li Zhu , Wu Liang , Zhong Fei , Cui Naxin , Dai Yanran , Cheng Shuiping 
Published: July 2017

AbstractTriazophos (TAP) is a widely used phosphorus pesticide in China that possesses a potential risk for water pollution. We have studied the removal efficiency of TAP using pilot-scale horizontal subsurface flow constructed wetlands (HSCWs) as well as the contribution of plants, substrates and other pathways to its removal. For TAP concentrations of 0.12 ± 0.04 mg L−1, 0.79 ± 0.29 mg L−1 and 3.96 ± 1.17 mg L−1, the removal efficiencies were 94.2 ± 3.7%, 97.8 ± 2.9% and 84.0 ± 13.5%, respectively, at a hydraulic loading rate (HLR) of 100 mm d−1; at an HLR of 200 mm d−1, the removal efficiencies were 96.7 ± 1.3%, 96.2 ± 1.7% and 61.7 ± 11.1%, respectively. The isopleth maps of TAP along the direction of flow indicate that most of the TAP removal occurred in the front and middle regions, while the major removal region would move forward with increasing influent TAP. Plant and substrate accumulation accounted for 0.035 ± 0.034% and 4.33 ± 0.43% of the total removal, respectively, indicating that over 95% of the TAP removal was achieved through other mechanisms. Thus, these results suggest HSCWs can be an effective approach with which to treat TAP contaminated water. Furthermore, the longitudinal scale and hydraulic conditions, as well as the roles of plants, substrates and microbes and their interactions, should be further considered in the design and application of CWs for pesticide pollution control.
Continuation of the genetic divergence of ecological speciation by spatial environmental heterogeneity in island endemic plants
Authors: Huang Bing-Hong , Huang Chih-Wei , Huang Chia-Lung , Liao Pei-Chun 
Published: July 2017

AbstractDivergent selection plays a critical role not only as a speciation driver but also in maintaining post-speciation divergence. In the absence of direct evidence, ancestral interspecific gene flow between incipient species can reflect ancient selective pressure for ecological speciation. In the present study, two late-Pleistocene diverged species endemic to Taiwan, Scutellaria playfairii and S. tashiroi, were spatially and ecologically partitioned with partial overlap. Multilocus genome-scan analyses and in silico evaluation revealed ancestral interspecific gene flow but distinct genetic compositions, implying that adaptive divergence contributed to their speciation. Ecological niche modeling and principal component analysis suggested incomplete divergent niches between the two species; the species distribution is therefore consistent with Hutchinson’s metaphor of multidimensional hypervolume niches rather than attributable to a single factor. Constraint ordination analysis supported this inference of a combination of variables explaining the genetic structure. The rare occurrence of hybrids in the sympatric population suggested hybrid breakdown, providing further evidence of divergent selection blocking gene flow. The correlation of environmental variables with integrated genetic components demonstrated that environmental heterogeneity maintains the species and population differentiation. This study highlights the importance of environmental heterogeneity and divergent selection for the rapid speciation and recent diversification of island plants.
Near infrared surface-enhanced Raman scattering based on star-shaped gold/silver nanoparticles and hyperbolic metamaterial
Authors: Lai Chih-Hsien , Wang Guo-An , Ling Tsung-Kai , Wang Tzyy-Jiann , Chiu Po-kai , Chou Chau Yuan-Fong , Huang Chih-Ching , Chiang Hai-Pang 
Published: July 2017

AbstractIt is desirable to extend the surface-enhanced Raman scattering (SERS) from the conventionally used visible range into the infrared region, because the fluorescence background is lower in the long-wavelength regime. To do this, it is important to have a SERS substrate suitable for infrared operation. In this work, we report the near infrared SERS operation based on the substrates employing star-shaped gold/silver nanoparticles and hyperbolic metamaterial (HMM) structure. We first fabricate the SERS substrate in which nanoparticles are separated from a silver film by a thin dielectric layer. Performance of the SERS substrate is investigated with a 1064-nm excitation source. Compared with similar silver film-based substrates employing respectively gold and silver spherical nanoparticles, it is found that, Raman intensity scattered by the substrate with star-shaped nanoparticles is 7.4 times stronger than that with gold nanoparticles, and 3.4 times stronger than that with silver nanoparticles. Following this, we fabricate the SERS substrate where the star-shaped nanoparticles are deposited over a HMM structure. The HMM structure comprises three pairs of germanium-silver multilayers. Further experimental result shows that, with the star-shaped nanoparticles, the HMM-based substrate yields 30% higher Raman intensity for near infrared SERS operation than the silver film-based substrate does.
Retrieving Precise Three-Dimensional Deformation on the 2014 M6.0 South Napa Earthquake by Joint Inversion of Multi-Sensor SAR
Authors: Jo Min-Jeong , Jung Hyung-Sup , Yun Sang-Ho 
Published: July 2017

AbstractWe reconstructed the three-dimensional (3D) surface displacement field of the 24 August 2014 M6.0 South Napa earthquake using SAR data from the Italian Space Agency’s COSMO-SkyMed and the European Space Agency’s Sentinel-1A satellites. Along-track and cross-track displacements produced with conventional SAR interferometry (InSAR) and multiple-aperture SAR interferometry (MAI) techniques were integrated to retrieve the east, north, and up components of surface deformation. The resulting 3D displacement maps clearly delineated the right-lateral shear motion of the fault rupture with a maximum surface displacement of approximately 45 cm along the fault’s strike, showing the east and north components of the trace particularly clearly. These maps also suggested a better-constrained model for the South Napa earthquake. We determined a strike of approximately 338° and dip of 85° by applying the Okada dislocation model considering a single patch with a homogeneous slip motion. Using the distributed slip model obtained by a linear solution, we estimated that a peak slip of approximately 1.7 m occurred around 4 km depth from the surface. 3D modelling using the retrieved 3D maps helps clarify the fault’s nature and thus characterize its behaviour.
Surprise routines in scientific writing
Authors: Tutin Agnès 
Published: July 2017

Emotions are almost absent from scientific articles except surprise, which plays a specific role in this genre. Surprise markers such as contrairement à nos
attentes (‘contrary to our expectations’) or ce résultat relativement surprenant (‘this somewhat surprising result’) are used in the framework of a scientific prediction model, implicit or explicitly formulated. A corpus linguistic study of adjectival and verbal markers allows us to determine several trends: (a) contrary to other genres such as novels or newspaper articles, surprise is not polar, that is to say it is neither positive nor negative, but stylistically “neutral”, (b) surprise is far more source-oriented than experiencer-oriented, (c) surprise generally involves the reader as a witness, and contributes, with other rhetorical markers, to “interlocutive” dialogism, (d) surprise often occurs in a prefabricated discursive scenario including several steps: (i) prediction model, (ii) observed facts, (iii) expression of surprise (or absence of surprise), (iv) explanation of surprising facts. Finally, we can question the status of surprise as an affect in scientific writing. It seems to be more a state of consciousness associated with the observation of complex facts. In any case, it appears to be a complex state, with rich conceptual content.