bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural models and considerations on the COA6, COX18 and COX20 factors that assist assembly of human cytochrome c oxidase subunit II
Authors: Abriata Luciano A 
Published: April 2017

AbstractThe soluble domain of cytochromecoxidase subunit II (COX2), located in the outer side of the inner mitochondrial membrane, contains a binuclear copper site (CuA) through which electrons flow from cytochromecto the core of the oxidase where oxygen reduction takes place. Being COX2 encoded in the mitochondrial genome, newly synthesized protein undergoes maturation steps in which it is translocated through and inserted into the inner mitochondrial membrane, and copper ions are loaded to form the CuA site. These steps are ensured by several protein factors in a complex pathway that is not fully understood, including copper-loading and disulfide-reduction proteins plus chaperones that assist proper membrane insertion. While the structure and function of copper-loading and disulfide-reducing proteins Sco1 and Sco2 have been quite studied at atomistic level, the latest biological studies have uncovered roles for other proteins that are not yet much understood at the structural level. In particular, recent experiments showed that membrane protein COX18 is a membrane-protein insertase for COX2, whereas membrane protein COX20 is a chaperone that stabilizes COX2 during translocation through the inner mitochondrial membrane, and soluble protein COA6 is part of the copper-loading pathway in conjunction with Sco1 and Sco2. This work reports structural models for COX18, COX20 and COA6, built judiciously from homology modeling, contact prediction-based modeling and transmembrane helix predictions, while considering the underlying biology. Implications and limitations of the models are discussed, and possible experimental routes to pursue are proposed. All models are provided as PyMOL sessions in the Supporting Information and can be visualized online athttp://lucianoabriata.altervista.org/modelshome.html.
Index switching causes “spreading-of-signal” among multiplexed samples in Illumina HiSeq 4000 DNA sequencing
Authors: Sinha Rahul , Stanley Geoff , Gulati Gunsagar S. , Ezran Camille , Travaglini Kyle J. , Wei Eric , Chan Charles K.F. , Nabhan Ahmad N. , Su Tianying , Morganti Rachel M. , Conley Stephanie D. , Chaib Hassan , Red-Horse Kristy , Longaker Michael T. , Snyder Michael P. , Krasnow Mark A. , Weissman Irving L. 
Published: April 2017

AbstractIllumina-based next generation sequencing (NGS) has accelerated biomedical discovery through its ability to generate thousands of gigabases of sequencing output per run at a fraction of the time and cost of conventional technologies. The process typically involves four basic steps: library preparation, cluster generation, sequencing, and data analysis. In 2015, a new chemistry of cluster generation was introduced in the newer Illumina machines (HiSeq 3000/4000/X Ten) called exclusion amplification (ExAmp), which was a fundamental shift from the earlier method of random cluster generation by bridge amplification on a non-patterned flow cell. The ExAmp chemistry, in conjunction with patterned flow cells containing nanowells at fixed locations, increases cluster density on the flow cell, thereby reducing the cost per run. It also increases sequence read quality, especially for longer read lengths (up to 150 base pairs). This advance has been widely adopted for genome sequencing because greater sequencing depth can be achieved for lower cost without compromising the quality of longer reads. We show that this promising chemistry is problematic, however, when multiplexing samples. We discovered that up to 5-10% of sequencing reads (or signals) are incorrectly assigned from a given sample to other samples in a multiplexed pool. We provide evidence that this “spreading-of-signals” arises from low levels of free index primers present in the pool. These index primers can prime pooled library fragments at random via complementary 3’ ends, and get extended by DNA polymerase, creating a new library molecule with a new index before binding to the patterned flow cell to generate a cluster for sequencing. This causes the resulting read from that cluster to be assigned to a different sample, causing the spread of signals within multiplexed samples. We show that low levels of free index primers persist after the most common library purification procedure recommended by Illumina, and that the amount of signal spreading among samples is proportional to the level of free index primer present in the library pool. This artifact causes homogenization and misclassification of cells in single cell RNA-seq experiments. Therefore, all data generated in this way must now be carefully re-examined to ensure that “spreading-of-signals” has not compromised data analysis and conclusions. Re-sequencing samples using an older technology that uses conventional bridge amplification for cluster generation, or improved library cleanup strategies to remove free index primers, can minimize or eliminate this signal spreading artifact.
Hypermarket private-label products, brand strategies and spokesperson persuasion
Authors: Chou Hsuan-Yi , Wang Tuan-Yu 
Published: April 2017

Purpose
The purpose of this paper is to investigate the effects of brand strategies and spokesperson expertise on consumer responses to hypermarket private-label products by combining concepts from consumer attitude change, resistance to persuasion and construal level theory (CLT).

Design/methodology/approach
Two experiments were conducted to test the propositions.

Findings
Consumers perceived the low-price (low-quality) characteristic of private-label products as a high-level (low-level) construal consideration when forming purchase decisions. Product relevance negatively affected consumers’ perceived product distance. Compared with store brands, separate brands enhanced consumer product attitudes and purchase intentions. Brand strategies and product distance affected consumer message-processing mindset (i.e. resistant to persuasion or open to persuasion) when processing advertisements, ultimately moderating the effect of spokesperson expertise.

Practical implications
The findings are useful for hypermarkets seeking to implement brand strategies and select spokespersons for private-label products. Additionally, the findings show that advertisers should design advertising elements to match consumers’ construal approaches to product-related information.

Originality/value
This study contrasts two common hypermarket brand strategies, identifies the construal levels corresponding to the dual roles of private-label products and expands CLT dimensions. Additionally, the results bridge two research approaches (persuasion and resistance to persuasion) and demonstrate the pivotal influence of brand strategies. The findings also advance understanding of the effects of spokesperson expertise and contribute to resistance theory by showing how to effectively reduce attitude certainty after resistance to persuasion.
Communication-in-use: customer-integrated marketing communication
Authors: Finne Åke , Grönroos Christian 
Published: April 2017

Purpose
This conceptual paper aims at developing a customer-centric marketing communications approach that takes the starting point in the customer ecosystem.

Design/methodology/approach
After a critical analysis of existing marketing communications and integrated marketing communication (IMC) approaches, a customer-driven view of marketing communications is developed using recent developments in relationship communication, customer-dominant logic and the notion of customer value formation as value-in-use.

Findings
A customer-integrated marketing communication (CIMC) approach centred on a communication-in-use concept is conceptually developed and introduced. The analysis results in a CIMC model, where a customer in his or her individual ecosystem, based on integration of a set of messages from different sources, makes sense of the many messages he or she is exposed to.

Research limitations/implications
The paper presents a customer-driven perspective on marketing communication and IMC. The analysis is conceptual and should trigger future empirical grounding. It indicates the need for a change in mindset in research.

Practical implications
CIMC requires a turnaround in the mindset that steers how companies and their marketers communicate with customers. The CIMC model provides guidelines for planning marketing communication.

Originality/value
The customer-driven communication-in-use concept and the CIMC model challenge traditional inside-out approaches to planning and implementing marketing communication.
Measuring integrated marketing communication by taking a broad organisational approach
Authors: Porcu Lucia , Del Barrio-García Salvador , Kitchen Philip J. 
Published: April 2017

PurposeThe purpose of this research is twofold: first, to conceptualise integrated marketing communication (IMC) by adopting a more inclusive and broader organisational perspective, and second, to empirically develop and validate a new measurement scale to assess firm-wide IMC.Design/methodology/approachThis paper is based on a multistage research design adopting qualitative and quantitative approaches. First, a comprehensive literature review and a two-round Delphi study served as the primary basis for the development of the IMC theoretical framework, including generation of items and content validation. Second, a pilot study (n= 39) enabled us to purify the measurement tool. Third, the data gathered via an online survey conducted among CEOs and other senior managers (n= 180) led to empirical validation of the proposed firm-wide IMC scale applying second-order confirmatory factor and structural equation modelling analyses.FindingsThis research produced the firm-wide IMC scale, a 25-item Likert-format measure exhibiting adequate dimensionality, reliability and construct (convergent, discriminant and nomological) validity.Originality/valueThe need for a more holistic approach emerged from both the academic literature and the professional arena. However, even very recent attempts to measure integration have involved the adoption of a narrow marketing communications-centred approach. Thus, the value and uniqueness of this paper lies in its novel definition of IMC as a four-dimensional construct and the development of a theoretically consistent, valid and reliable measurement tool for the assessment of integration based on a firm-wide organisational approach.
IMC education and digital disruption
Authors: Kerr Gayle , Kelly Louise 
Published: April 2017

Purpose
The purpose of this paper is to benchmark the progress of integrated marketing communication (IMC) education, by replicating and extending a study on IMC education by Kerr (2009). It documents progress, examines the impact of digital disruption and concludes with an agenda for change.

Design/methodology/approach
Using the Delphi technique, this study recruited leading IMC educators and thought leaders internationally to find consensus on an important range of IMC issues, including its place within the university, the IMC brand, curriculum, impact on practice, the incorporation of digital and future challenges.

Findings
IMC has strengthened its place within the university and also within the minds and understanding of academics, students and industry. Digital disruption provides many challenges including updating curriculum and up skilling staff. It is vital, however, that IMC thinking be positioned as the integrator and digital as the facilitator, providing platforms to actualise IMC strategy such as content, customer service and cross-functional planning.

Practical implications
This study shows what IMC education has achieved since Kerr’s (2009) study. Further, it outlines what needs to be achieved in the future by providing a “To do” list for IMC educators.

Originality/value
It is vital that the development and progress of this important new area of study is tracked to ensure industry challenges are met, such as digital disruption, and the right education for IMC managers of the future is provided.
Illuminating the black box of entrepreneurship education programmes: Part 2
Authors: Maritz Alex 
Published: April 2017

Purpose
The purpose of this paper is to provide a justified, legitimate and validated model on entrepreneurship education programmes (EEPs), by combining recent research and scholarship in leading edge entrepreneurship education (EE).

Design/methodology/approach
A systematic literature review of recent EE research and scholarship is followed by an empirical study to develop a model of EEPs. This was adopted by employing an emergent inquiry perspective incorporating participatory action research, using frame analysis and NVIVO to develop and analyse themes.

Findings
This research identified three unique dimensions currently excluded from the theoretical and scholarship of EEPs, being distinct contextualisation, entrepreneurship ecosystems and recent content innovation in entrepreneurship. It also identified updates to current EEPs dimensions, such as online technologies, authentic alignment, causation, effectuation and bricolage, technology transfer the entrepreneurial university.

Research limitations/implications
The discussion and model presented in this paper may be a starting point for future empirical studies on EEPs, by developing additional validation, justification and legitimisation.

Practical implications
The study indicates that EEPs are integrative and dynamic, and always limited to contextual and contemporary inferences; providing guidance to developing such programmes. Hence, the applicability to update the original framework developed by Maritz and Brown (2013) as Part 2 of illuminating the black box of EEPs.

Originality/value
This paper provides a first of its kind empirical study in the development of EEPs frameworks and models, deepening the theory, scholarship and development of such models.
Code-to-code verification of an axisymmetric model of the Bridgman solidification process for alloys
Authors: Seredyński Mirosław , Battaglioli Sara , Mooney Robin P. , Robinson Anthony J. , Banaszek Jerzy , McFadden Shaun 
Published: April 2017

Purpose
Numerical models of manufacturing processes are useful and provide insight for the practitioner; however, model verification and validation are a prerequisite for expedient application. This paper aims to detail the code-to-code verification of a thermal numerical model for the Bridgman solidification process of alloys in a two-dimensional axisymmetric domain, against an established commercial code (ANSYS Fluent); the work is considered a confidence building step in model development.

Design/methodology/approach
A grid sensitivity analysis is carried out to establish grid independence, and this is followed by simulations of two transient solidification scenarios: pulling rate step change and ramp input; the results of which are compared and discussed.

Findings
Good conformity of results is achieved; hence, the non-commercial model is code-to-code verified; in addition, the ability of the non-commercial model to deal with radial heat flow is demonstrated.

Originality/value
The ability of the home made model for Bridgman furnace solidification to deal with cases where significant radial heat transfer occurs in the sample was demonstrated. The introduction of front tracking to model the macroscopic growth of dendritic mush and the region of undercooled liquid is identified as the next step in model development.
Numerical model of a shaft furnace operation
Authors: Straka Robert , Telejko Tadeusz 
Published: April 2017

Purpose
The model of a shaft furnace operation is presented in this paper. Aim of this model is to predict concentrations of carbon monoxide and dioxide, the temperature of the lava and the heat losses.

Design/methodology/approach
The mathematical model is based on 1D mass and heat balance laws for flue gas, coke and four materials used in a mineral wool production. Process parameters should be optimized for the minimal heat loss and the carbon monoxide concentration while keeping the prescribed lava temperature. The model consists of heterogeneous and homogeneous reactions for coke combustion, dolomite decomposition, rock and coke heating and a rock-melting model. The resulting system of partial differential equations is discretized by the finite volume method and solved with the explicit Euler scheme together with the point-implicit preconditioning of sources in species balance equations.

Findings
Numerical results are compared with the measured data on the pilot-scale device and show good agreement. It is found that in the lower region of the furnace, the large amount of carbon monoxide is present despite high oxygen levels.

Practical/implications
Based on the numerical model, the parameters of the secondary air stream could be studied (position, volume flux, oxygen enrichment and temperature) to decrease levels of carbon monoxide emissions while keeping lava temperature at needed levels.

Originality/value
The paper includes mathematical and numerical model needed for simulation of shaft furnaces in mineral wool industry. It can be used as a valuable tool for design engineers and furnace operators during research or redesign of existing devices.
Assessment of causative factors for project abandonment in Nigerian public tertiary educational institutions
Authors: Alao Oluwaseyi Olalekan , Jagboro Godwin Onajite 
Published: April 2017

Purpose
Abandonment of construction projects is still a burning issue in Nigeria. Beside the poor financing of educational infrastructure, abandonment of construction projects remains a significant contributor to the inadequacy of facilities in Nigerian public tertiary educational institutions. The purpose of this paper is, therefore, to assess the causes of abandoned projects specific to public institutions of tertiary education in Nigeria, with a view to providing empirical data that are generalizable to enhancing successful delivery of teaching and research facilities.

Design/methodology/approach
Primary data used for the study were obtained through questionnaires administered to 47 professionals comprising 8 architects, 12 mechanical and electrical engineers, 15 civil/structural engineers, 4 builders and 8 quantity surveyors who were involved in physical development of construction projects in public tertiary educational institutions in Osun State. The data were analyzed using mean analysis, factor analysis and the Kruskal-Wallis (K-W) test.

Findings
The factors most significant to abandonment of tertiary educational institutional projects were delayed payments, fund mismanagement, inadequate budgetary allocation, inadequacy of finance, inflation and bankruptcy of the contractor. Findings also showed that not all factors causing abandonment were significant to tertiary institutional projects. The significant factors clustered under stakeholders’ response capacity, poor financial management, inadequate planning and monitoring, and unexpected occurrences. The K-W test showed significant differences among the categories of tertiary institutions on the ranking of the most significant causes of abandoned projects.

Research limitations/implications
The study was limited to public tertiary educational institutions in Osun State. Further studies could focus on public health institution projects and private tertiary educational projects to improve the body of knowledge on the subject of causative factors for project abandonment.

Practical implications
The study provided implications for effective contract management of public tertiary educational institutional projects, which is a significant step to improving the available teaching and research facilities in Nigerian tertiary institutions.

Originality/value
The study provides implications for effective contract management systems of projects for public tertiary educational institutions, thereby improving the available teaching and research facilities.
Individualized male dress shirt adjustments using a novel method for measuring shoulder shape
Authors: Kim KyoungOk , Innami Noriaki , Takatera Masayuki , Narita Tadaharu , Kanazawa Midori , Kitazawa Yuji 
Published: April 2017

Purpose
The purpose of this paper is to investigate the current challenges to making individualized men’s dress shirt adjustments, and devised a novel measurement method to assess shoulder shape.

Design/methodology/approach
To understand the common complaints about ready-to-wear dress shirts, a wear evaluation with 15 Japanese males was performed. The shoulder components of these shirts could not be adjusted using any currently available measurement methods. The three-dimensional body shape of two subjects who had a problem with the shoulder of ready-to-wear shirts was compared with a dummy designed to represent the average Japanese male.

Findings
The authors determined that one of the subjects with an incompatible shoulder fit had a shoulder point (SP) line that was anterior to the one measured on the average dummy. The other subject had a smaller shoulder angle than the average dummy. To effectively measure the wearer’s shoulder characteristics, the authors devised a new measuring device that can measure the shoulder angle and its degree of forward thrust. With this device, it was possible to understand the wearers’ shoulder types and make appropriate dress shirt adjustments.

Originality/value
The authors devised a new measuring device to assess shoulder angle and forward thrust, qualities that previously could not be measured without three-dimensional analysis. Using this device, it was possible to understand the wearers’ shoulder types and make appropriate dress shirt adjustments.
Preferences in tracking dimensions for wearable technology
Authors: Koo Helen Sumin , Fallon Kris 
Published: April 2017

Purpose
The purpose of this paper is to understand what dimensions consumers prefer to track using wearable technology to achieve a healthier lifestyle and how these tracking dimensions are related.

Design/methodology/approach
An online survey was conducted with potential consumers in the USA, and a series of Pearson’s correlation and regression analysis and multiple regressions was conducted.

Findings
The most preferred self-tracking dimensions, tracking dimensions on others, most private tracking dimensions, most variable dimensions, and the dimensions that need to be improved were identified. The results of this study showed positive relationships overall among similar types of tracking dimensions, such as among dimensions of physical health condition (disease and disorder symptoms and general vital signs), mental health condition (stress level and mood/feeling), healthy lifestyle (fitness, and pose and posture), and productivity and task management (work productivity, location, and time management).

Originality/value
Designers are encouraged to make wearable technology products that are durable, easy to care for, attractive in design, comfortable to wear and use, able to track preferred dimensions, appropriate for various consumers, unobtrusive, portable, and small. This research will guide wearable technology and fashion industry professionals in the development process of wearable technology to benefit consumers by helping them be more self-aware, empowering them to develop a healthier lifestyle, and ultimately increasing their quality of life and well-being.