bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New 35-mm Short Intra-aortic Balloon Catheter: A Suitable Option also for Non-Small-Sized Patients?
Authors: Gelsomino Sandro , Lozekoot Pieter Willem , Lorusso Roberto , de Jong Monique Maria , Parise Orlando , Matteucci Francesco , Lucà Fabiana , Kumar Narendra , Dehkord Eshan Sadeghian , Romano Salvatore Mario , Gensini Gian Franco , La Meir Mark , Maessen Josephus Gregorius 
Published: February 2016

Objective Visceral ischemia can be a potentially life-threatening complication of intra-aortic balloon pump (IABP) support. A shorter IABP catheter might lead to a reduction of visceral complications. In this animal study, we evaluate the effects of a 35-mL short catheter in comparison with a 40-mL standard-sized catheter. Methods Eighteen healthy swine underwent 120-minute ligation of the left anterior descending coronary artery followed by 6 hours of reperfusion being supported by either a short IABP catheter (short group) (n = 6) or a long IABP catheter (long group) (n = 6) or with no assistance (controls) (n = 6). Hemodynamics, visceral and coronary flows, as well as biochemical markers were evaluated throughout the different phases of the protocol. Results Mesenteric flows increased significantly at reperfusion (P < 0.001 both) remaining constant afterward (all, P > 0.05) in the short group, while remaining significantly lower in the long group at the start of reperfusion, remaining constantly lower than the short group and controls (P < 0.001 vs short, P < 0.003 vs controls). In both long and short groups, catheters improved renal flows at reperfusion (P < 0.001 both) without any further variation (P < 0.05). In the short group, the flows were higher during the whole of reperfusion (all, P < 0.05). Intra-aortic balloon pump support improved hemodynamic indices and coronary blood flows during reperfusion to a similar extent in both the small and the long group (P > 0.05). Conclusions The short IABP catheter proved to be as effective as the standard-sized catheter in supporting hemodynamics and coronary circulation. Furthermore, it even improves visceral flows in comparison with conventional IABP catheters.
Preoperative pain mechanisms assessed by cuff algometry are associated with chronic postoperative pain relief after total knee replacement
Authors: Petersen Kristian Kjær , Graven-Nielsen Thomas , Simonsen Ole , Laursen Mogens Berg , Arendt-Nielsen Lars 
Published: February 2016

AbstractChronic postoperative pain after total knee replacement (TKR) in knee osteoarthritis (KOA) implies clinical challenges. Widespread hyperalgesia, facilitated temporal summation of pain (TSP), and impaired conditioned pain modulation (CPM) have been found in painful KOA. This exploratory study investigated postoperative pain relief 12 months after TKR in 4 subgroups of patients preoperatively profiled by mechanistic quantitative sensory testing. In 103 patients with KOA, pressure pain detection threshold (PDT) and tolerance thresholds (PTT) were assessed at the lower leg using cuff algometry. Temporal summation of pain was measured as an increase in pain intensity scores during 10 repeated (2 seconds intervals) painful cuff stimuli. Conditioned pain modulation was calculated as the relative increase in PDT during painful conditioning stimulation. The grand averages of TSP and CPM were calculated and values below or above were used for subgrouping: facilitated TSP/impaired CPM (group A, N = 16), facilitated TSP/normal CPM (group B, N = 15), normal TSP/impaired CPM (group C, N = 44), and normal TSP/normal CPM (group D, N = 28). Clinical VAS pain intensity scores were collected before and 12 months after TKR surgery and the pain relief calculated. Less pain relief was found in group A (52.0% ± 14.0% pain relief) than in group B (81.1% ± 3.5%,P= 0.023) and group C (79.6% ± 4.4%,P= 0.007), but not group D (69.4% ± 7.9%,P= 0.087). Low preoperative PDT was associated with a less postoperative pain relief (R= −0.222,P= 0.034), whereas TSP or CPM alone showed no associations with postoperative pain relief. This explorative study indicated that patients with osteoarthritis with facilitated TSP together with impaired CPM are more vulnerable to experience less pain relief after TKR.
Lower sex hormone levels are associated with more chronic musculoskeletal pain in community-dwelling elderly women
Authors: de Kruijf Marjolein , Stolk Lisette , Zillikens M. Carola , de Rijke Yolanda B. , Bierma-Zeinstra Sita M.A. , Hofman Albert , Huygen Frank J.P.M. , Uitterlinden Andre G. , van Meurs Joyce B.J. 
Published: February 2016

Abstract
Chronic pain is more prevalent in women than in men, with increasing differences between sexes in advanced age. This could be caused by differences in sex hormone levels. We therefore studied the relationship between sex hormones and the prevalence and incidence of chronic pain. The association between sex hormone levels and chronic pain was examined in 9717 participants aged 45 years and older from the Rotterdam Study, a population-based study. Chronic pain was defined as pain in the lower back, hands, knees and/or hips for at least 3 months. Sex hormone levels included estrogen, testosterone, androstenedione, and 17-hydroxyprogesterone. Relationships between hormones and prevalent and new onset chronic pain were analyzed using linear and logistic regression, stratified by gender. Women with androstenedione or estradiol levels in the lowest tertile had more chronic pain (odds ratio, 1.20; 95% CI, 1.03-1.39 and odds ratio, 1.27; 95% CI, 1.10-1.48, respectively). Mean estradiol levels were lower among men with chronic pain (mean difference −3.88 pmol/L; P = 0.005). Lowest tertile 17-hydroxyprogesterone in women was associated with 38% more new onset pain. All these associations were independent from age, body mass index, health and lifestyle factors, and osteoarthritis. Lower sex hormone levels are associated with chronic musculoskeletal pain, independent from lifestyle and health-related factors, in community-dwelling elderly women. These results suggest that sex hormones play a role in chronic pain and should be taken into account when a patient presents with chronic pain. Therefore, sex hormones may be a potential treatment target for these patients.
Demoralization in Cancer Patients and Related Factors in Taiwan
Authors: Li Yu-Chi , Ho Chung-Han , Wang Hsiu-Hung 
Published: February 2016

Background:
Demoralization frequently occurs in hospice, cancer, and critically ill patients. Severe demoralization can lead to suicidal ideation, making this issue of great import to healthcare providers.

Objective:
The aim of this study is to inform nursing professionals of the risk factors of demoralization in cancer patients via investigating its relationship with cancer patients’ demographic data and disease characteristics.

Methods:
This is a cross-sectional descriptive study using a structured questionnaire including assessments of demographic data and disease characteristics, as well as the Demoralization Scale Mandarin version. Univariate logistic regression was used to explore the relations between demoralization and these other variables.

Results:
The mean (SD) Demoralization Scale Mandarin version score was 30.08 (13.68) (range, 0–73). Demoralization was significantly related to age (r = 0.1, P = .050), marital status (r = 0.11, P = .034), education (r = 0.17, P < .001), monthly income (r = 0.22, P < .001), disease status (r = 0.10, P = .050), and treatment type (r = 0.12, P = .014).

Conclusions:
This study demonstrates the factors influencing demoralization among cancer patients. Future studies might expand to include patients with other chronic or critical illnesses or disadvantaged groups to better understand the prevalence of demoralization. This would help draw more attention from clinical healthcare providers, healthcare institutions, and other healthcare authorities to demoralization.

Implications or Practice:
The results provide reference data for nursing professionals about the care of cancer patients.
Developing Normal Turns–Amplitude Clouds for Upper and Lower Limbs
Authors: Jabre Joe F. , Nikolayev Sergey G. , Babayev Michael B. , Chindilov Denis V. , Muravyov Anatoly Y. 
Published: February 2016

Purpose:
Turns and amplitude analysis (T&A) is a frequently used method for automatic EMG interference pattern analysis. The T&A normal values have only been developed for a limited number of muscles. Our objective was to obtain normal T&A clouds for upper and lower extremity muscles for which no normal values exist in the literature.

Methods:
The T&A normative data using concentric needle electrodes were obtained from 68 men and 56 women aged 20 to 60 years.

Results:
Normal upper and lower extremity T&A clouds were obtained and presented in this article.

Conclusion:
The T&A normal values collected in this study maybe used to detect neurogenic and myopathic abnormalities in men and women at low-to-moderate muscle contractions. The effect of turns–amplitude data obtained at high force level of muscle contraction and its potential to falsely show neurogenic abnormalities are discussed.
Evaluation of Techniques for Performing Cellular Isolation and Preservation during Microgravity Conditions
Authors: Rizzardi Lindsay F. , Kunz Hawley , Rubins Kathleen , Chouker Alexander , Quiriarte Heather , Sams Clarence , Crucian Brian E. , Feinberg Andrew P. 
Published: February 2016

AbstractGenomic and epigenomic studies require the precise transfer of small volumes from one container to another. Epigenomic and transcriptional analysis require separation of purified cell types, and long term preservation of cells requires their isolation and transfer into appropriate freezing media. There are currently no protocols for these procedures on the ISS. Currently samples are either frozen as mixed cell populations, with poor yield, or returned under ambient conditions, requiring timing with Soyuz missions. Here, we evaluate the feasibility of translating terrestrial cell purification techniques to the ISS. Our evaluations were performed in microgravity conditions during parabolic atmospheric flight. The pipetting of open liquids in microgravity was evaluated using analog blood fluids and several types of pipette hardware. The best performing pipettes were used to evaluate the pipetting steps required for peripheral blood mononuclear cell (PBMC) isolation via density gradient centrifugation (DGC). Evaluation of actual blood products was performed for both the overlay of diluted blood, and the extraction of isolated PBMCs. We also validated magnetic purification of cells. We found that positive displacement pipettes avoided air bubbles, and the tips allowed the strong surface tension of water, glycerol and blood to maintain a patent meniscus and withstand robust pipetting in microgravity. These procedures will greatly increase the breadth of research that can be performed onboard the ISS, and allow improvised experimentation on extraterrestrial missions.
Sentinel versus passive surveillance for measuring changes in dengue incidence: Evidence from three concurrent surveillance systems in Iquitos, Peru
Authors: Olkowski Sandra , Stoddard Steven T. , Halsey Eric S. , Morrison Amy C. , Barker Christopher M. , Scott Thomas W. 
Published: February 2016

AbstractMonitoring changes in infectious disease incidence is fundamental to outbreak detection and response, intervention outcome monitoring, and identifying environmental correlates of transmission. In the case of dengue, little is known about how consistently surveillance data track disease burden in a population over time. Here we use four years of monthly dengue incidence data from three sources – population-based (‘passive’) surveillance including suspected cases, ‘sentinel’ surveillance with 100% laboratory confirmation and complete reporting, and door-to-door (‘cohort’) surveillance conducted three times per week - in Iquitos, Peru, to quantify their relative consistency and timeliness. Data consistency was evaluated using annual and monthly expansion factors (EFs) as cohort incidence divided by incidence in each surveillance system, to assess their reliability for estimating disease burden (annual) and monitoring disease trends (monthly). Annually, passive surveillance data more closely estimated cohort incidence (average annual EF=5) than did data from sentinel surveillance (average annual EF=19). Monthly passive surveillance data generally were more consistent (ratio of sentinel/passive EF standard deviations=2.2) but overestimated incidence in 26% (11/43) of months, most often during the second half of the annual high season as dengue incidence typically wanes from its annual peak. Increases in sentinel surveillance incidence were correlated temporally (correlation coefficient = 0.86) with increases in the cohort, while passive surveillance data were significantly correlated at both zero-lag and a one-month lag (0.63 and 0.44, respectively). Together these results suggest that, rather than relying on a single data stream, a clearer picture of changes in infectious disease incidence might be achieved by combining the timeliness of sentinel surveillance with the representativeness of passive surveillance.
Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction
Authors: Shurtleff Matthew , Karfilis Kate V. , Temoche-Diaz Morayma , Ri Sayaka , Schekman Randy 
Published: February 2016

AbstractExosomes are small vesicles that are secreted from metazoan cells and may convey selected membrane proteins and small RNAs to target cells for the control of cell migration, development and metastasis. To study the mechanisms of RNA packaging into exosomes, we devised a purification scheme based on the membrane marker CD63 to isolate a single exosome species secreted from HEK293T cells. Using immunoisolated CD63-containing exosomes we identified a set of microRNAs that are highly enriched with respect to their cellular levels. To explore the biochemical requirements for exosome biogenesis and RNA packaging, we devised a cell-free reaction that recapitulates the species-selective enclosure of miR-223 in isolated membranes supplemented with cytosol. We found that the RNA-binding protein Y-box protein I (YBX1) binds to and is required for the sorting of miR-223 in the cell-free reaction. Furthermore, YBX1 serves an important role in the secretion of miRNAs in exosomes by HEK293T cells.
A universal model for carbon dioxide uptake by plants
Authors: Wang Han , Prentice I. Colin , Cornwell William K. , Keenan Trevor F. , Davis Tyler W. , Wright Ian J. , Evans Bradley J. , Peng Changhui 
Published: February 2016

AbstractThe rate of carbon uptake by land plants depends on the ratio of leaf-internal to ambient carbon dioxide partial pressures1, here termedχ. This quantity is a key determinant of both primary production and transpiration and the relationship between them. But current models forχare empirical and incomplete, contributing to the many uncertainties afflicting model estimates and future projections of terrestrial carbon uptake2,3. Here we show that a simple evolutionary optimality hypothesis4,5generates functional relationships betweenχand growth temperature, vapour pressure deficit and elevation that are precisely and quantitatively consistent with empiricalχvalues from a worldwide data set containing > 3500 stable carbon isotope measurements. A single global equation embodying these relationships then unifies the empirical light use efficiency model with the standard model of C3photosynthesis1, and successfully predicts gross primary production as measured at flux sites. This achievement is notable because of the equation′s simplicity (with just two parameters, both independently estimated) and applicability across biomes and plant functional types. Thereby it provides a theoretical underpinning, grounded in eco-evolutionary principles, for large-scale analysis of the CO2and water exchanges between atmosphere and land.
Kronos: a workflow assembler for genome analytics and informatics
Authors: Taghiyar M Jafar , Rosner Jamie , Grewal Diljot , Grande Bruno , Aniba Rad , Grewal Jasleen , Boutros Paul C , Morin Ryan D , Bashashati Ali , Shah Sohrab P 
Published: February 2016

AbstractBackgroundThe field of next generation sequencing informatics has matured to a point where algorithmic advances in sequence alignment and individual feature detection methods have stabilized. Practical and robust implementation of complex analytical workflows (where such tools are structured into ‘best practices’ for automated analysis of NGS datasets) still requires significant programming investment and expertise.ResultsWe presentKronos, a software platform for automating the development and execution of reproducible, auditable and distributable bioinformatics workflows. Kronos obviates the need for explicit coding of workflows by compiling a text configuration file into executable Python applications. The framework of each workflow includes a run manager to execute the encoded workflows locally (or on a cluster or cloud), parallelize tasks, and log all runtime events. Resulting workflows are highly modular and configurable by construction, facilitating flexible and extensible meta-applications which can be modified easily through configuration file editing. The workflows are fully encoded for ease of distribution and can be instantiated on external systems, promoting and facilitating reproducible research and comparative analyses. We introduce a framework for building Kronos components which function as shareable, modular nodes in Kronos workflows.ConclusionThe Kronos platform provides a standard framework for developers to implement custom tools, reuse existing tools, and contribute to the community at large. Kronos is shipped with both Docker and Amazon AWS machine images. It is free, open source and available through PyPI (Python Package Index) andhttps://github.com/jtaghiyar/kronos.
Metaproteomics reveals biomarkers of system collapse in a dynamic aquatic ecosystem
Authors: Northrop Amanda C. , Brooks Rachel , Ellison Aaron M. , Gotelli Nicholas J. , Ballif Bryan A. 
Published: February 2016

AbstractForecasting and preventing rapid ecosystem state changes is important but hard to achieve without functionally relevant early warning indicators. Here we use metaproteomic analysis to identify protein biomarkers indicating a state change in an aquatic ecosystem resulting from detrital enrichment. In a 14-day field experiment, we used detritus (arthropod prey) to enrich replicate aquatic ecosystems formed in the water-filled pitcher-shaped leaves of the northern pitcher plant,Sarracenia purpurea. Shotgun metaproteomics using a translated, custom metagenomic database identified proteins, molecular pathways, and microbial taxa that differentiated control oligotrophic ecosystems that captured only ambient prey from eutrophic ecosystems that were experimentally enriched. The number of microbial taxa was comparable between treatments; however, taxonomic evenness was higher in the oligotrophic controls. Aerobic and facultatively anaerobic bacteria dominated control and enriched ecosystems, respectively. The molecular pathways and taxa identified in the enriched treatments were similar to those found in a wide range of enriched or polluted aquatic ecosystems and are derived from microbial processes that occur at the base of most detrital food webs. We encourage the use of metaproteomic pipelines to identify better early-warning indicators of impending changes from oligotrophic to eutrophic states in aquatic and other detrital-based ecosystems.
Dermoscopy of Grover's disease: Variability according to histological subtype
Authors: Errichetti Enzo , De Francesco Vincenzo , Pegolo Enrico , Stinco Giuseppe 
Published: February 2016

AbstractDistinguishing Grover's disease from other papular dermatoses is often a troublesome task. According to two relatively recent case reports, dermoscopy may be useful in assisting the diagnosis of such a disorder by showing a peculiar pattern. The aim of this study was to evaluate the dermoscopic features of Grover's disease in a larger series of patients and correlate dermoscopy with histopathological findings. Seven patients with histopathologically confirmed Grover's disease were included in the study, with three of them displaying a Darier‐like histology and the remaining four cases having a spongiotic histological pattern. The results of our study suggest that Grover's disease may display different features according to the histological subtype, with a central star‐shaped/branched polygonal/roundish‐oval brownish area surrounded by a whitish halo being characteristic of the Darier‐like histological subtype and whitish scales over a reddish‐yellowish background being characteristic of the spongiotic histological subtype.
Three cases of pigmented cosmetic dermatitis‐like eruptions associated with primary Sjögren's syndrome or anti‐SSA antibody
Authors: Takeo Naoko , Sakai Takashi , Saito‐Shono Tomoko , Ishikawa Kazushi , Hatano Yutaka , Katagiri Kazumoto , Takahashi Yoshihiro , Kawano Kenji , Kimoto Kenichi , Kubota Toshiaki , Eshima Nobuoki , Kojima Hiroto , Fujiwara Sakuhei 
Published: February 2016

AbstractPigmented cosmetic dermatitis‐like (Riehl's melanosis‐like) pigmentation was reported in three of 27 patients with primary Sjögren's syndrome. But case reports of such eruptions are rare. We describe three cases of such eruptions associated with primary Sjögren's syndrome or anti‐SSA antibody and possible associations with specific types of human leukocyte antigen (HLA) and infiltrating lymphocytes. These middle‐aged Japanese women had reticular facial pigmentation and histopathological examination revealed interface dermatitis, melanophages, and dense lymphocytic infiltration around hair follicles and sweat ducts. HLA typing revealed common antigenic equivalents or genetic typing of HLA‐A2, DR52, DPA1(02:02) and DPB1(05:01). Immunohistochemical staining revealed major subsets of T cells to be CD8 and CD45RO. Some Foxp3‐ and few IL17‐positive cells were found in strong contrast to the major CD4 subset of infiltrated T cells in annular erythema associated with Sjögren's syndrome. Apparently, our patients' pigmentation represented a specific etiology associated with primary Sjögren's syndrome or anti‐SSA antibody.
Presence of antidrug antibodies correlates inversely with the plasma tumor necrosis factor (TNF)‐α level and the efficacy of TNF‐inhibitor therapy in psoriasis
Authors: Kui Róbert , Gál Brigitta , Gaál Magdolna , Kiss Mária , Kemény Lajos , Gyulai Rolland 
Published: February 2016

AbstractAntidrug antibodies have been shown to be associated with a loss of response during biologic therapy. Despite the potential association, there has been no report on the simultaneous monitoring of the following parameters in psoriasis: presence of neutralizing antibodies, plasma tumor necrosis factor (TNF)‐α concentration, TNFi concentration and disease activity. Plasma concentrations of adalimumab, infliximab, etanercept and their respective antidrug antibodies, as well as plasma concentrations of TNF‐α were measured in 77 psoriasis patients receiving biologic therapy, and the values were correlated with the clinical activity of the skin disease. Antidrug antibodies were identified in the plasma of 25% of infliximab‐treated patients and 29.6% of adalimumab‐treated patients, but not in the etanercept group. Clinical severity scores were significantly higher in the antibody‐positive patients. In patients receiving infliximab or adalimumab therapy, the presence of antidrug antibodies was directly associated with reduced plasma TNF‐inhibitor concentration and elevated plasma TNF‐α level.