bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical limitations of the International Federation of Gynecology and Obstetrics (FIGO) classification of uterine fibroids
Authors: Laughlin‐Tommaso Shannon K. , Hesley Gina K. , Hopkins Matthew R. , Brandt Kathleen R. , Zhu Yunxiao , Stewart Elizabeth A. 
Published: July 2017

AbstractObjectiveTo determine the reproducibility of classifying uterine fibroids using the 2011 International Federation of Gynecology and Obstetrics (FIGO) staging system.MethodsThe present retrospective cohort study included patients presenting for the treatment of symptomatic uterine fibroids at the Gynecology Fibroid Clinic at Mayo Clinic, Rochester, USA, between April 1, 2013 and April 1, 2014. Magnetic resonance imaging of fibroid uteri was performed and the images were independently reviewed by two academic gynecologists and two radiologists specializing in fibroid care. Fibroid classifications assigned by each physician were compared and the significance of the variations was graded by whether they would affect surgical planning.ResultsThere were 42 fibroids from 23 patients; only 6 (14%) fibroids had unanimous classification agreement. The majority (36 [86%]) had at least two unique answers and 4 (10%) fibroids had four unique classifications. Variations in classification were not associated with physician specialty. More than one‐third of the classification discrepancies would have impacted surgical planning.ConclusionFIGO fibroid classification was not consistent among four fibroid specialists. The variation was clinically significant for 36% of the fibroids. Additional validation of the FIGO fibroid classification system is needed.
Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex
Authors: Wang Lifang , Shen Yanbing , Liu Mengjiao , Tang Rui , Wang Min 
Published: July 2017

AbstractBACKGROUNDIonic liquids (ILs) have become increasingly attractive as alternatives for organic solvents. In this study, the influences of ILs on the cellular growth rate, cellular activity, cortisone acetate (CA) conversion, CA solubility, and cell membrane permeability of Arthrobacter simplex CPCC 140451 (ASP) were examined.RESULTSResults showed that ILs with ≥3 carbon atoms in their side chains increased CA conversion, CA solubility, and cell membrane permeability of ASP and universally inhibited the cellular growth rate and activity, with ILs with [PF6]‐anion exhibiting the most severe inhibition activity. Inhibition was also more serious using ILs with longer alkyl side chains and at higher concentrations. CA conversion, CA solubility, and leakage of cell inclusions showed higher increase under ILs with [PF6]‐anion than those of ILs with [BF4]‐anion and lower increase for ILs with longer side chains.CONCLUSIONThis study improved the understanding of the influence and primary selection of imidazolium‐based ILs when applied in steroid biotransformation with Arthrobacter simplex whole cells. The data provided a foundation for the selection of suitable ILs for various applications. © 2017 Society of Chemical Industry
Optimal Use of Transient Elastography and Acoustic Radiation Force Impulse to Stage Liver Fibrosis in HIV/HCV‐Coinfected Patients in Clinical Practice
Authors: López Juan José , Pérez‐Àlvarez Núria , Rodríguez Raul V. , Jou Antoni , Carbonell Pere , Jiménez José A. , Soldevila Laura , Tenesa Montserrat , Tor Jordi , Clotet Bonaventura , Bechini Jordi , Tural Cristina 
Published: July 2017

ObjectivesLiver fibrosis (LF) is crucial for the individualized management of patients with hepatitis C virus (HCV). We evaluated the concordance between two noninvasive methods for staging LF, transient elastography (TE) and acoustic radiation force impulse (ARFI), in patients coinfected with human immunodeficiency virus and HCV. We propose an algorithm for optimal use of both techniques in routine clinical practice.MethodsA total of 89 human immunodeficiency virus/HCV‐coinfected patients underwent TE and ARFI on the same day. The kappa index was used to assess concordance between the techniques. An algorithm combining ARFI and TE was proposed based on the independent factors associated with a kappa index greater than or equal to 0.70, obtained from a multiple regression analysis. We performed a cost‐effectiveness analysis. The study was approved by our institutional review board and all patients signed the informed consent.ResultsConcordance between TE and ARFI for F2, F3, and F4 was 0.55, 0.59, and 0.69, respectively. Ultrasound normal spleen size (odds ratio [OR], 0.20; 95% confidence interval [CI], 0.05–0.91) and high viral load (OR, 0.36; 95% CI, 0.17–0.77) reduced the probability of agreement between TE and ARFI, whereas ultrasound normal left liver lobe size (OR, 3.32; 95% CI, 1.21–9.10) increased this probability. The algorithm revealed that LF was adequately assessed in 74.16%, with 25.84% of patients misclassified. The incremental cost‐effectiveness ratio of TE compared with ARFI to increase concordance by 1% was €8.86.ConclusionsConcordance between TE and ARFI was moderate. In the algorithm we proposed, ARFI was cost‐effective as a first technique for the staging of LF in the study population.
Association Between Endometrial/Subendometrial Vasculature and Embryo Transfer Outcome: A Meta‐analysis and Subgroup Analysis
Authors: Wang Jianing , Xia Fei , Zhou Ying , Wei Xuedong , Zhuang Yanyan , Huang Yingxue 
Published: July 2017

ObjectivesTo examine the association between endometrial/subendometrial vasculature and in vitro fertilization‐embryo transfer (IVF‐ET) and frozen embryo transfer (FET) outcomes.MethodsA meta‐analysis of studies using endometrial/subendometrial 3‐dimensional ultrasound and power Doppler angiography was performed to examine the vascularization index (VI), flow index (FI), and vascularization‐flow index (VFI) in pregnant and nonpregnant women. Ten articles were analyzed, including 895 pregnant women and 882 nonpregnant women.ResultsA subgroup analysis of the measuring time showed that the endometrial VI (standardized mean difference [SMD], 0.57; 95% confidence interval [CI], 0.40, 0.74; P < .00001), FI (SMD, 0.56; 95% CI, 0.33, 0.78; P < .00001), and VFI (SMD, 0.45; 95% CI, 0.28, 0.61; P < .00001) measured on the ET day, but not on the human chorionic gonadotropin (hCG) trigger day, were significantly higher in pregnant than nonpregnant women. Additionally, the subendometrial FI was significantly increased in pregnant women on the both hCG day (SMD, 0.68; 95% CI, 0.31, 1.06; P = .004) and ET day (SMD, 0.30; 95% CI, 0.08, 0.52; P = .007). A subgroup analysis of cycle type showed that the endometrial VI (SMD, 0.52; 95% CI, 0.30, 0.74; P < .00001), FI (SMD, 0.44; 95% CI, 0.22, 0.66; P = .0001), and VFI (SMD, 0.45; 95% CI, 0.23, 0.67; P = .03) on the ET day were significantly increased in pregnant women in the FET subgroup.ConclusionsThe subendometrial FI on the hCG day and endometrial VI, FI, and VFI on the ET day are potentially associated with pregnancy occurrence during IVF‐ET. The endometrial VI, FI, and VFI could help identify appropriate timing for FET. However, the accuracy of these indices in predicting pregnancy occurrence must be further evaluated in additional large‐scale studies.
Otolaryngology externships and the match: Productive or futile?
Authors: Thomas Carissa M. , Cabrera‐Muffly Cristina 
Published: July 2017

Objectives/HypothesisThe objectives of this study were to summarize externship experiences among recent graduates and current residents in otolaryngology residency programs and determine whether externships affect the match process.Study DesignCross‐sectional survey.MethodsA survey was distributed to otolaryngology residents in allopathic US residency programs and otolaryngology graduates from the past 5 years (2011–2015). There were 2,141 surveys distributed.ResultsThere were 654 subjects who responded, for a 30.5% response rate. Most respondents were residents (n = 438, 67%). Of the residents, 85.6% had completed at least one externship compared to 75.9% of graduates (P = .002). The most common reasons for selecting a particular externship were geographic location (74.2%) and program reputation (71.1%), whereas the most common reason for not completing an externship was being advised not to (59.1%). Furthermore, 82.6% of respondents received at least one interview from their externships, 90% went to those interviews, and 89.1% reported that externship experiences affected their rank list. Respondents had a 32.7% match rate to the externship residency program if it was ranked versus a 48.8% match rate if the program was ranked first. Respondents who matched at the externship residency program matched higher on their rank list (P < .001). Of the respondents, 90.7% found externships to be valuable, and 74.5% recommended completing one.ConclusionsExternships are beneficial because they influence the rank list of applicants and are viewed as valuable experiences. Completing an externship is advisable for the experience, but applicants should weigh the benefits versus the possible risk of being judged more harshly during a month‐long rotation.Level of EvidenceNA Laryngoscope, 127:2242–2246, 2017
Laryngeal distribution of recurrent respiratory papillomatosis in a previously untreated cohort
Authors: Benedict Peter A. , Ruiz Ryan , Yoo MiJin , Verma Avanti , Ahmed Omar H. , Wang Binhuan , Dion Gregory R. , Voigt Andrew , Merati Albert , Rosen Clark A. , Amin Milan R. , Branski Ryan C. 
Published: July 2017

Objectives/HypothesisTo describe the distribution of recurrent respiratory papillomatosis (RRP) lesions across 21 laryngeal anatomic regions in previously untreated patients at initial presentation to provide insight regarding the natural history of RRP.Study DesignMulti‐institutional, retrospective case series.MethodsInitial laryngoscopic examination videos of 83 previously untreated patients with adult‐onset RRP were reviewed. Papilloma locations were recorded using a 21‐region laryngeal schematic. Multivariate analyses by anatomic subsite were conducted for the entire population and for subgroups stratified by sex, age, and proton pump inhibitor (PPI) usage. Heat maps were generated, hierarchically color coding the anatomic distribution of disease.ResultsIn this cohort, RRP was most likely to occur on the true vocal folds (TVFs) and anterior commissure (P < .0001, odds ratio [OR]: 7.02); within the TVFs, the membranous vocal folds (MVFs) were most likely to be affected (P < .0001, OR: 3.56). The cohort was predominantly male (80.7%); males had a higher average number of affected sites (P = .005) and were more likely to have lesions in any laryngeal subsite (P < .0001, OR: 2.88,) compared to females. PPI users were more likely than nonusers to have disease in any laryngeal subsite (P = .0037, OR: 1.62), particularly in the posterior and subglottic regions (P = .0061, OR: 2.53). Age was not correlated with lesion prevalence or distribution.ConclusionsIn untreated patients presenting to three laryngology clinics, the MVFs were most likely to be affected by RRP. Males had more anatomic sites affected by papilloma than females. The influence of PPI use on RRP distribution warrants further investigation.Level of Evidence4. Laryngoscope, 128:138–143, 2018
Surgical outcome for empty nose syndrome: Impact of implantation site
Authors: Lee Ta‐Jen , Fu Chia‐Hsiang , Wu Ching‐Lung , Lee Yi‐Chan , Huang Chi‐Che , Chang Po‐Hung , Chen Yi‐Wei , Tseng Hsiao‐Jung 
Published: July 2017

Objective/HypothesisEndonasal submucosal implantation has been confirmed to be beneficial for patients with empty nose syndrome (ENS). However, the optimal implantation site has not been defined. This study aimed to evaluate whether lateral nasal wall implantation is superior to inferior nasal wall implantation in terms of clinical benefits and improvements in quality of life.Study DesignRetrospective study in a tertiary medical center.MethodsConsecutive ENS patients between 2010 and 2015 with operative histories of inferior turbinectomies and indicated for surgical implantation were enrolled, with at least 1‐year follow‐up. Patients were divided into lateral and inferior nasal wall groups. SinoNasal Outcome Test (SNOT)‐22, Beck Depression Inventory (BDI)‐II, and Beck Anxiety Inventory (BAI) were applied before and 1 year after implantation.ResultsOf the total 30 ENS patients analyzed, 14 were in the inferior nasal wall group and 16 were in the lateral nasal wall group. There were no significant intergroup differences in demographic data and preoperative SNOT‐22, BDI‐II, and BAI scores. Postoperative assessment revealed that the lateral nasal wall group had significantly better SNOT‐22 score improvements than the inferior nasal wall group, particularly regarding rhinological symptoms and sleep function.ConclusionLateral nasal wall implantation may provide significantly better clinical outcomes than inferior nasal wall implantation, and thus may be the preferred, more optimal site for implant placement in ENS patients.Level of Evidence4. Laryngoscope, 128:554–559, 2018
A1S_2811, a CheA/Y‐like hybrid two‐component regulator from Acinetobacter baumannii ATCC17978, is involved in surface motility and biofilm formation in this bacterium
Authors: Chen Rong , Lv Ruichen , Xiao Lisheng , Wang Min , Du Zongmin , Tan Yafang , Cui Yujun , Yan Yanfeng , Luo Yanping , Yang Ruifu , Song Yajun 
Published: July 2017

AbstractTwo‐component systems in Acinetobacter baumannii are associated with its virulence, drug resistance, motility, biofilm formation, and other characteristics. In this study, we used RecAb, a genetic engineering method, to investigate the function of A1S_2811 in A. baumannii strain ATCC17978. A1S_2811, a hypothetical hybrid sensor histidine kinase/response regulator, has four histidine‐containing phosphotransfer domains, a CheA‐like regulatory domain, and a CheY‐like receiver domain at its C terminus. Compared with the ATCC17978 strain, both surface motility and biofilm formation at the gas–liquid interface decreased significantly in the A1S_2811 knock‐out strain. The number of pilus‐like structures and the amount of extrapolymeric substances on the cell surface also decreased in the A1S_2811 null strain. Transcription of abaI, which encodes an N‐acylhomoserine lactone synthase in A. baumannii , decreased significantly in the A1S_2811 null strain, and supplementation with synthetic N‐(3‐oxodecanoyl) homoserine‐l‐lactone rescued the surface motility and biofilm formation phenotype in the null mutant. We speculate that A1S_2811 regulates surface motility and biofilm formation, not by regulating type IV pili‐associated genes expression, but by regulating the chaperone/usher pili‐associated csuA/ABCDE operon and the AbaI‐dependent quorum‐sensing pathway‐associated A1S_0112‐0119 operon instead.
Variability of 4D flow parameters when subjected to changes in MRI acquisition parameters using a realistic thoracic aortic phantom
Authors: Montalba Cristian , Urbina Jesus , Sotelo Julio , Andia Marcelo E. , Tejos Cristian , Irarrazaval Pablo , Hurtado Daniel E. , Valverde Israel , Uribe Sergio 
Published: July 2017

PurposeTo assess the variability of peak flow, mean velocity, stroke volume, and wall shear stress measurements derived from 3D cine phase contrast (4D flow) sequences under different conditions of spatial and temporal resolutions.MethodsWe performed controlled experiments using a thoracic aortic phantom. The phantom was connected to a pulsatile flow pump, which simulated nine physiological conditions. For each condition, 4D flow data were acquired with different spatial and temporal resolutions. The 2D cine phase contrast and 4D flow data with the highest available spatio‐temporal resolution were considered as a reference for comparison purposes.ResultsWhen comparing 4D flow acquisitions (spatial and temporal resolution of 2.0 × 2.0 × 2.0 mm3 and 40 ms, respectively) with 2D phase‐contrast flow acquisitions, the underestimation of peak flow, mean velocity, and stroke volume were 10.5, 10 and 5%, respectively. However, the calculated wall shear stress showed an underestimation larger than 70% for the former acquisition, with respect to 4D flow, with spatial and temporal resolution of 1.0 × 1.0 × 1.0 mm3 and 20 ms, respectively.ConclusionsPeak flow, mean velocity, and stroke volume from 4D flow data are more sensitive to changes of temporal than spatial resolution, as opposed to wall shear stress, which is more sensitive to changes in spatial resolution. Magn Reson Med 79:1882–1892, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
Cationic Supramolecular Hydrogels for Overcoming the Skin Barrier in Drug Delivery
Authors: Limón David , Jiménez‐Newman Claire , Rodrigues Mafalda , González‐Campo Arántzazu , Amabilino David B. , Calpena Ana C. , Pérez‐García Lluïsa 
Published: July 2017

AbstractA cationic bis‐imidazolium‐based amphiphile was used to form thermoreversible nanostructured supramolecular hydrogels incorporating neutral and cationic drugs for the topical treatment of rosacea. The concentration of the gelator and the type and concentration of the drug incorporated were found to be factors that strongly influenced the gelling temperature, gel‐formation period, and overall stability and morphology. The incorporation of brimonidine tartrate resulted in the formation of the most homogeneous material of the three drugs explored, whereas the incorporation of betamethasone resulted in a gel with a completely different morphology comprising linked particles. NMR spectroscopy studies proved that these gels kept the drug not only at the interstitial space but also within the fibers. Due to the design of the gelator, drug release was up to 10 times faster and retention of the drug within the skin was up to 20 times more effective than that observed for commercial products. Experiments in vivo demonstrated the rapid efficacy of these gels in reducing erythema, especially in the case of the gel with brimonidine. The lack of coulombic attraction between the gelator–host and the guest–drug seemed particularly important in highly effective release, and the intermolecular interactions operating between them were found to lie at the root of the excellent properties of the materials for topical delivery and treatment of rosacea.
Highly Atom Economic Synthesis of d‐2‐Aminobutyric Acid through an In Vitro Tri‐enzymatic Catalytic System
Authors: Chen Xi , Cui Yunfeng , Cheng Xinkuan , Feng Jinhui , Wu Qiaqing , Zhu Dunming 
Published: July 2017

Abstractd‐2‐Aminobutyric acid is an unnatural amino acid serving as an important intermediate in pharmaceutical production. Developing a synthetic method that uses cheaper starting materials and produces less by‐product is a pressing demand. A tri‐enzymatic catalytic system, which is composed of l‐threonine ammonia lyase (l‐TAL), d‐amino acid dehydrogenase (d‐AADH), and formate dehydrogenase (FDH), has thus been developed for the synthesis of d‐2‐aminobutyric acid with high optical purity. In this cascade reaction, the readily available l‐threonine serves as the starting material, carbon dioxide and water are the by‐products. d‐2‐Aminobutyric acid was obtained with >90 % yield and >99 % enantioselective excess, even without adding external ammonia, demonstrating that the ammonia from the first reaction can serve as the amino donor for the reductive amination step. This multi‐enzymatic system provides an attractive method with high atomic economy for the synthesis of d‐α‐amino acids from the corresponding l‐α‐amino acids, which are readily produced by fermentation.
Regulation of the activity of the promoter of RNA‐induced silencing, C3PO
Authors: Sahu Shriya , Williams Leo , Perez Alberto , Philip Finly , Caso Giuseppe , Zurawsky Walter , Scarlata Suzanne 
Published: July 2017

AbstractRNA‐induced silencing is a process which allows cells to regulate the synthesis of specific proteins. RNA silencing is promoted by the protein C3PO (component 3 of RISC). We have previously found that phospholipase Cβ, which increases intracellular calcium levels in response to specific G protein signals, inhibits C3PO activity towards certain genes. Understanding the parameters that control C3PO activity and which genes are impacted by G protein activation would help predict which genes are more vulnerable to downregulation. Here, using a library of 1018 oligonucleotides, we show that C3PO binds oligonucleotides with structural specificity but little sequence specificity. Alternately, C3PO hydrolyzes oligonucleotides with a rate that is sensitive to substrate stability. Importantly, we find that oligonucleotides with higher Tm values are inhibited by bound PLCβ. This finding is supported by microarray analysis in cells over‐expressing PLCβ1. Taken together, this study allows predictions of the genes whose post‐transcriptional regulation is responsive to the G protein/phospholipase Cβ/calcium signaling pathway.
Renal neuroendocrine control of desiccation and cold tolerance byDrosophila suzukii
Authors: Terhzaz Selim , Alford Lucy , Yeoh Joseph GC , Marley Richard , Dornan Anthony J , Dow Julian AT , Davies Shireen A 
Published: July 2017

AbstractBACKGROUNDNeuropeptides are central to the regulation of physiological and behavioural processes in insects, directly impacting cold and desiccation survival. However, little is known about the control mechanisms governing these responses inDrosophila suzukii. The close phylogenetic relationship ofD. suzukiiwithDrosophila melanogasterallows, through genomic and functional studies, an insight into the mechanisms directing stress tolerance inD. suzukii.RESULTSCapability (Capa), leucokinin (LK), diuretic hormone 44 (DH44) and DH31neuropeptides demonstrated a high level of conservation betweenD. suzukiiandD. melanogasterwith respect to peptide sequences, neuronal expression, receptor localisation, and diuretic function in the Malpighian tubules. DespiteD. suzukii's ability to populate cold environments, it proved sensitive to both cold and desiccation. Furthermore, inD. suzukii,Capaacts as a desiccation‐ and cold stress‐responsive gene, whileDH44gene expression is increased only after desiccation exposure, and theLKgene after nonlethal cold stress recovery.CONCLUSIONThis study provides a comparative investigation into stress tolerance mediation by neuroendocrine signalling in twoDrosophilaspecies, providing evidence that similar signalling pathways control fluid secretion in the Malpighian tubules. Identifying processes governing specific environmental stresses affectingD. suzukiicould lead to the development of targeted integrated management strategies to control insect pest populations. © 2017 The Authors.Pest Management Sciencepublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
The effect of YSi2 nanoinclusion on the thermoelectric properties of p‐type SiGe alloy
Authors: Wongprakarn Suphagrid , Pinitsoontorn Supree , Tanusilp Sora‐at , Kurosaki Ken 
Published: July 2017

In this research, the p‐type Si80Ge20B3 alloys composited with YSi2 nanoinclusions were fabricated by melting spinning and then, spark plasma sintering. The metallic Y was chosen as the source of YSi2 nanoparticles. It was found that the fully dense nanocomposites were formed with the homogeneous distribution of YSi2 nanoinclusions in the SiGe matrix. The thermoelectric measurement showed that YSi2 addition reduces the electrical conductivity but increases the Seebeck coefficient, which was attributed to the decrease in carrier concentration. The thermal conductivity was suppressed for the composite SiGe‐1.4%Y, which made this composition to exhibit the largest thermoelectric figure‐of‐merit (ZT) of 0.52.Figure‐of‐merit (ZT) and microstructure of the SiGe‐x%Y SiGe‐1.4%Y.