bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factores que predicen la rehabilitación satisfactoria en pacientes con amputación de miembro inferior de un instituto de rehabilitación
Authors: Quezada Ponte Elisa , Montalvo Chávez Rosa Sabina 
Published: September 2024

Objetivo: Determinar los factores que predicen la rehabilitación satisfactoria en pacientes con amputación de miembro inferior que obtuvieron una prótesis. Material y métodos: Estudio observacional, retrospectivo y relacional, muestreo no probabilístico por conveniencia de 70 pacientes con amputación de miembro inferior de origen vascular, oncológico o traumático, con niveles de amputación transfemoral (AK) o transtibial (BK), en el Instituto Nacional de Rehabilitación “Dra. Adriana Rebaza Flores” AMISTAD PERU-JAPON, Lima-Perú, entre el 2018 y 2019. Se revisaron las historias clínicas y se realizaron entrevistas. La evaluación de la rehabilitación satisfactoria se realizó usando el “Instrumento de Houghton”. Resultados: El 81,42% de los pacientes eran de sexo masculino, edad media de 48,76 ± 15,19 años y el 35,71% tenía entre 48 y 57 años. Se encontró relación estadísticamente significativa de la rehabilitación satisfactoria con los siguientes factores: edad (p < 0, 011), nivel de amputación BK (p < 0,001), etiología traumática (p < 0,014) y ausencia de comorbilidad (p < 0,04). El único factor predictor de la rehabilitación satisfactoria fue el nivel de amputación BK OR= 2,84 (p < 0,002). Conclusiones: Los factores que predicen una rehabilitación satisfactoria en el paciente con amputación de miembro inferior fueron edad menor de 48 años, nivel de amputación BK, etiología traumática, menor comorbilidad. El único factor determinante fue el nivel de amputación.
Effects of obesity and obesity-related phenotypes on diffuse idiopathic skeletal hyperostosis: A Mendelian randomization analysis
Authors: Xu Qing , Li Mufeng , Wang Zhonghan , Jiao Jianhang , Yang Lili , Jiang Weibo , Yu Tong , Wang Yang , Wu Minfei 
Published: October 2024

Abstract

The study endeavored to investigate the potential genetic causality between obesity or obesity-related phenotypes and diffuse idiopathic skeletal hyperostosis, with the genome-wide association study aggregated data. The linkage disequilibrium score regression approach, Mendelian randomization analysis and Bayesian colocalization were applied to test the causality. For the linkage disequilibrium score regression analysis, besides the waist-to-hip ratio, the results showed significant genetic association between obesity-related phenotypes and diffuse idiopathic skeletal hyperostosis. For the Mendelian randomization analysis, there were significant causal relationships between body mass index, waist circumference, hip circumference, whole body fat mass, body fat percentage, arm fat mass and percentage (left), arm fat mass and percentage (right), leg fat mass and percentage (left), leg fat mass and percentage (right), trunk fat percentage (All the above p = 0.000), trunk fat mass (p = 0.011, p value adjusted by Benjaminiad Hochberg = 0.011 < 0.05) and diffuse idiopathic skeletal hyperostosis, but not for waist-to-hip ratio. For the Bayesian colocalization analysis, hip circumference, whole body fat mass, body fat percentage, and leg fat percentage (right) shared the same variants with diffuse idiopathic skeletal hyperostosis. This study provided evidence that genetic-related increases in obesity or partial obesity-related phenotype risks have positive effects on diffuse idiopathic skeletal hyperostosis.
Elucidating the Causal Impact of Plasma Proteins on Osteoporosis Risk and the Mediating Role of Immune Cells through Integrated Multi-omics Data Analysis
Authors: Ji Zhongjie , Li Xiang , Ma Jinlong , Sun Zhenqian , Wu Wenliang , Chen Yunzhen , Wang Hongliang , You Yunhao , Jiao Guangjun 
Published: October 2024

Abstract

Recent evidence increasingly suggests that plasma proteins and immune factors play crucial roles in bone metabolism. However, the specific mechanisms of how plasma proteins impact osteoporosis (OP) and whether immune factors mediate the process remain largely unknown. In this study, we employed a two sample mendelian randomization (TSMR) model and bidirectional analysis to identify plasma proteins linked to OP, explore whether immune phenotypes mediate OP risk associated with these proteins. Our findings revealed that plasma MGP and TMEM38B proteins were negatively correlated with OP (p < 0.001), while CD14, RUFY1, and IL6ST were positively associated with the risk of OP (p < 0.001). Further validation using whole blood eQTL data revealed a positive association between CD14 and OP risk (OR = 1.254, 95% CI: 1.016–1.547, p < 0.05). The analysis of the GEO dataset further corroborated this finding. Analysis revealed 62 immune phenotypes related to OP. Bidirectional TSMR analysis showed a positive correlation between plasma protein CD14 and IgD + CD38-%B cells (Ivw beta = 0.349, OR = 1.418, 95% CI: 1.068–1.882, p < 0.05). In addition, mediation analysis results showed that IgD + CD38-%B cells mediates 12% of the effect of CD14 on OP risk. Furthermore, Bayesian colocalization analysis reinforced our findings. Finally, in the drug target MR study and Phe-MR analysis, anti-CD14 exhibited a protective effect against OP (OR = 0.798, 95%CI: 0.646–0.984, p < 0.05). Our study provides evidence that elevated plasma CD14 is linked to OP risk, partially mediated by IgD + CD38-%B cells. Blocking CD14 shows potential in preventing or delaying OP. Further research is warranted to elucidate the underlying physiological and pathological mechanisms.
Impact of Gypsum and Filter Cake on Selected Physicochemical Properties of Saline Sodic Soil of Amibara Area Central Rift Valley Ethiopia
Authors: Yeshibelay Agered , Woji Lemma , Habte Dawit 
Published: October 2024

Abstract

Salinity and aridity are two interrelated problems rapidly expanding in Ethiopia and soil with saline sodic property in Amibara irrigated farms threatens crop productivity. A field experiment was conducted at Werer Agricultural Research Center to evaluate the ameliorative effects of gypsum and filter cake on saline-sodic soil. Composite surface soil samples before the experiment and plot-level samples after harvest were collected for laboratory analysis. Post-harvest soil analysis revealed that bulk density, soil pH, exchangeable sodium, and exchangeable calcium, were significantly affected by the interaction of gypsum and filter cake (P < 0.01). The lowest mean soil pH (7.76) was measured from plots treated with 75% gypsum requirement + 25 t ha− 1 filter cakes and the highest soil pH (8.20) was recorded from untreated plots. Experimental plots treated with a sole application of gypsum and filter cake showed a reduction of exchangeable sodium percentage (ESP). In conclusion, the combined amendment of gypsum and filter cake can improve the adverse impacts of pH, SAR, exchangeable sodium, and exchangeable sodium percentage. It could be concluded that the combined application of Gypsum and filter cake enhances soil physicochemical properties and wheat production in the saline-sodic soil of Amibara District.
Characterisation of a novel metal-containing “glyco-protein/polypeptide-organochlorine” bioflocculant produced from Pseudomonas aeruginosa strain F29 isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Oyo State, Nigeria
Authors: Okorie Ikechukwu Kenneth M. , Ogunjobi Adeniyi A. 
Published: October 2024

Abstract

Antibiotic resistance has reached global proportions, and the discovery of effective alternatives to the common antibiotics in current use, could aid in solving this problem. The aim of this study was to characterise a bioflocculant produced from Pseudomonas aeruginosa strain F29, accession number OQ734844, that possessed 69% flocculating activity, and that had been observed to demonstrate biocontrol activity against Staphylococcus aureus SO183 at concentrations of 0.090 g/L and 0.150 g/L, and against identified Pseudomonas aeruginosa, at a concentration of 0.150 g/L. Pseudomonas aeruginosa strain F29, was isolated from pig fecal matter collected from a mixed animal farm in Ibadan, Nigeria via the pour plate method, identified through biochemical tests and confirmed through molecular studies. Fourier transform infrared spectroscopy (FTIR) of the bioflocculant, revealed the presence of carboxyl, amide/peptide, aromatic alcohol, alkene, halo and polysulfide functional groups. Scanning electron microscopy (SEM) revealed a clumped and flaky bioflocculant surface, while energy dispersive X-ray spectroscopy (EDX) revealed the presence of chlorine (56.00%), carbon (20.50%), sodium (12.50%), oxygen (4.00%), phosphorus (3.00%), sulphur (2.43%) magnesium (1.06%), potassium (0.32%) and nitrogen (0.30%). High performance liquid chromatography (HPLC) and mass spectrometry (MS) revealed that the bioflocculant possessed varied peaks of glucose, galactose, inositol and mannose, D-ribose, arabinose, rhamnose and xylose. The phenol sulfuric acid method revealed that the concentration of these sugars was 0.0059 g/L. The bioflocculant was a metal-containing polymeric compound composed mainly of carbohydrates, proteins/polypeptides and organochlorines, possibly a metal-containing “glyco-protein/polypeptide organochlorine.” From available documentation, this is the first report of this type of bioflocculant.
Causes of mechanical intestinal obstructions and diagnosis in adults
Authors: khalefa Basel Al , Alhwamda Mohammad 
Published: October 2024

Abstract

Objective Intestinal obstruction is defined as the presence of a blockage in the intestines that prevents the passage of contents, slowing down or halting the digestive process. It can be either mechanical or paralytic. Therefore, this study aims to evaluate mechanical intestinal obstructions, accurately determine their causes, and diagnose them in adults.Methods and Materials: A retrospective study was conducted, including a sample of 197 patients admitted to the surgery department at Damascus Hospital due to mechanical intestinal obstruction, from June 2021 to June 2024. The sample included patients aged over 18 years, from both genders. Data analysis was performed by a statistical expert.Results In our study, there were 128 male patients and 69 female patients, with an average age of 45 years. The most common age group was 18–30 years. We found that the most common cause of intestinal obstruction was adhesions (73.51%), followed by hernias (12.19%) and tumors (6.28%). The most frequent symptom was cessation of gas and stool passage (82.2%), followed by abdominal pain (78.7%) and then nausea and vomiting. The most important signs were abdominal distension (70.7%) and abdominal wall swelling (12.2%). The most commonly used diagnostic tool was a standing abdominal X-ray, utilized in 98.2% of cases. Management included bowel resection and intestinal anastomosis for tumors, while fecal impaction was managed surgically. A group of patients was managed conservatively.Conclusion Establishing a protocol that defines the symptoms and signs of intestinal strangulation would be highly beneficial for the early diagnosis of adhesion-related intestinal obstructions. This would enable earlier treatment, reducing complications and mortality.
Supporting the development of climate-resilient water safety plans through a risk-screening framework for drinking water source protection
Authors: Vorstius Carolin , Rowan John S. , Brown Iain , Leith Fraser , Frogbrook Zoë 
Published: October 2024

ABSTRACT
Climate change-induced impacts on water-quality challenge risk management of drinking water. Uncertainty around future changes and limited resources require approaches that enable prioritisation at regional or national scale for planning and adaptive intervention. This paper presents a framework for risk screening drinking water supplies, providing a staged approach to building the capacity for integrating climate change into risk management. The framework is applied to drinking water sources of the Scottish national drinking water provider, developing raw water trajectories for indicative water-quality parameters (water colour and Escherichia coli). It establishes baseline understanding of water-quality controls through multivariate statistical analysis. It incorporates climate and land-use change into the model using climate (UKCP18) and land-use projections. The results suggest potential higher organic carbon production in soils from increased production with higher temperature, combined with reduced dilution and drought effects from reductions in summer rainfall. For E. coli, risks arise through land-use change leading to potential agricultural intensification. The framework supports the targeted development of climate-resilient Water Safety Plans, prioritising systems most at risk of raw water-quality deteriorations. It emphasises source protection to maintain or improve regulatory compliance, minimise socioeconomic and environmental harms, and generate additional benefits.
Water resources in Pakistan: a comprehensive overview and management challenges
Authors: Nazli Sana , Liu Jiahong , Wang Haibin , Soomro Shan-e-hyder 
Published: October 2024

ABSTRACT
Pakistan has a range of water resources, including many rivers, large glaciers in the Himalayas, and significant underground water reserves, located throughout the country. However, the country faces considerable challenges in effectively managing these water resources. Employing a comprehensive review methodology, this paper examines policy documents and recent scholarly articles to analyze the current obstacles and propose viable solutions for sustainable water management in Pakistan. Significant challenges, including delayed policy implementation, insufficient regulatory enforcement, and inadequate data collection, hinder water resource management. Furthermore, there is a critical gap in integrating climate adaptation measures into water management strategies, which affects the resilience and efficiency of water resource allocation and utilization amid changing environmental conditions. It recommends significant policy changes that include economic measures such as water pricing, advancements in water recycling technologies, and approaches to improve climate resilience. Furthermore, this study proposes a comprehensive framework designed to tackle the complex challenges of water management. It suggests an integrated approach that combines policy, technology, environmental, and economic strategies to promote sustainable and resilient water use.
Retinoic Acid Improves Vascular Endothelial Dysfunction by Inhibiting PI3K/AKT/YAP-mediated Ferroptosis in Diabetes Mellitus
Authors: Zhang Man , Liu Yun , Liu Yu , Tang Bailin , Wang Hongxin , Lu Meili 
Published: October 2024

Background:
Vascular endothelial dysfunction is the initial factor involved in cardiovascular injury
in patients with diabetes. Retinoic acid is involved in improving vascular complications with diabetes, but its
protective mechanism is still unclear. This study aimed to evaluate the effect and mechanism of All-Trans Retinoic
Acid (ATRA) on endothelial dysfunction induced by diabetes.

Methods:
In the present study, streptozotocin (STZ)-induced diabetic rats and high glucose (HG)-induced human
umbilical vein endothelial cells (HUVECs) were observed, and the effects of ATRA on HG-induced endothelial
dysfunction and ferroptosis were evaluated.

Results:
ATRA treatment improved impaired vasorelaxation in diabetic aortas in an endothelium-dependent
manner, and this effect was accompanied by an increase in the NO concentration and eNOS expression. Ferroptosis,
characterized by lipid peroxidation and iron overload induced by HG, was improved by ATRA administration,
and a ferroptosis inhibitor (ferrostatin-1, Fer-1) improved endothelial function to a similar extent as
ATRA. In addition, the inactivation of phosphoinositol-3-kinase (PI3K)/protein kinases B (AKT) and Yes-Associated
Protein (YAP) nuclear localization induced by HG were reversed by ATRA administration. Vascular
ring relaxation experiments showed that PI3K/AKT activation and YAP inhibition had similar effects on ferroptosis
and endothelial function. However, the vasodilative effect of retinoic acid was affected by PI3K/AKT
inhibition, and the inhibitory effects of ATRA on ferroptosis and the improvement of endothelial function
were dependent on the retinoic acid receptor.

Conclusion:
ATRA could improve vascular endothelial dysfunction by inhibiting PI3K/AKT/YAP-mediated
ferroptosis induced by HG, which provides a new idea for the treatment of vascular lesions in diabetes.
Limit Detection of Short Tandem Repeats (STR) Analysis on Touch DNA Samples
Authors: Saamia Vira , Yudianto Ahmad , Nurjayadi Muktiningsih , Novitasari Novitasari , Furqoni Abdul Hadi 
Published: October 2024

Forensic short tandem repeats (STR) profiling on touch DNA samples has emerged as a primary method for human identification. The stability and uniqueness of STR combination from the targeted locus in each individual make it a precision marker for human identification. Touch DNA samples can be found in traces of biological material shed from a person. This work aimed to identify the lowest concentration limit required for generating an interpretable DNA profile and the sensitivity of the STR loci applied. Touch DNA samples were collected from donors who were asked to hold a rope for 5 min. A double swab technique was used to lift the touch samples from the rope. These samples are subjected to DNA extraction and quantification. Two STR amplification cycles, 29 and 34 cycles, were used. DNA concentration greatly influences the success of amplifying the target allele at each STR locus to be interpreted into a complete DNA profile, shown by its allele peak. Touch DNA concentration >0.25 ng can produce a complete DNA profile. LCN method successfully amplified touch DNA with a concentration 0.0625–0.25 ng/µL. Limit detection of touch DNA analysis is 0.25 ng/µL. Low-copy DNA can still be analyzed within 0.0625–0.25 ng/µL.
DFT Insights into the Structural, Mechanical, Electronic and Optical Properties of Novel InZnCl<sub>3</sub> and InCdCl<sub>3</sub> Chloro-Perovskites
Authors: Pingak Redi Kristian , Ngara Zakarias Seba , Johannes Albert Zicko , Bukit Minsyahril , Tanesib Jehunias Leonidas , Nitti Fidelis , Sianturi Hery Leo , Pasangka Bartholomeus 
Published: October 2024

The ABX3 perovskite materials have recently emerged as one of the most promising materials for optoelectronic applications. In the present study, novel perovskites in the form of InZnCl3 and InCdCl3 are computationally investigated to determine their key characteristics, including structural, mechanical, electronic, and optical characteristics. These characteristics were evaluated using the density functional theory (DFT) implemented in the quantum espresso code. The results indicated that both materials exhibit chemical, dynamic, and mechanical stability. Moreover, these perovskites are predicted to be ductile, rendering them suitable for a broad array of optoelectronic applications, including solar cells. The electronic band structure and the density of states of the materials revealed their characteristics as indirect semiconductors with band gap energy values of 0.96 eV for InZnCl3 and 1.83 eV for InCdCl3 perovskites. The optical properties calculations also unveiled that these perovskites possess strong absorption in the visible-ultraviolet spectrum (up to 106 cm−1) and low reflectivity. The calculated refractive index and extinction coefficient of the compounds were also predicted in this study. These collective findings strongly suggest the potential applications of these novel materials in optoelectronic devices.
Green Synthesis of Silver Nanoparticles with Bioreductant from Lime Juice Powder (<i>Citrus aurantifolia</i>): Effect of Concentration and pH
Authors: Mulyana Septian Dwi , Sari Retno , Rijal Agus Syamsur 
Published: October 2024

Significant development in antibacterial agents derived from metal nanoparticles is currently underway. One commonly employed type is silver nanoparticles (AgNPs), which display potential antibacterial activity at lower concentrations compared to other metals. Due to their high surface-to-volume ratio and small size, AgNPs can readily penetrate bacterial cell walls. Green synthesis methods of AgNPs, such as utilizing plants as reducing agents, offer substantial advantages over other synthesis techniques. Lime (Citrus aurantifolia), containing compounds like flavonoids and saponins, can serve as a natural reducing agent, converting Ag+ ions to Ag0. This study aims to evaluate the effects of AgNO3 concentration, lime juice powder concentration, and pH on the formation and characteristics of AgNPs, as well as their activity against the bacteria Propionibacterium acnes, Staphylococcus aureus, and Escherichia coli. Results indicate that at an AgNO3 concentration of 1 mM, lime juice extract at 1% with a pH of 9 produces optimal AgNP formation, with an absorbance of 4.631 and particle size of 68.4 nm. AgNPs exhibit higher antibacterial activity than AgNO3. AgNPs synthesized with lime juice powder can increase their activity and have the advantages of being safe and environmentally friendly since they use plant material as a reducing agent.
High Temperature Oxidation Behavior of ODS Ferritic Stainless Steel Fe-16Cr-4Al-1Ni-0.4Y<sub>2</sub>O<sub>3</sub>
Authors: Wafda Hakimul , Prajitno Djoko Hadi , Basuki Eddy Agus , Syafiq Ahmad , Widiawati Nina , Andi Mustari Asril Pramutadi 
Published: October 2024

This study investigates the isothermic oxidation behavior of the new ODS alloy Fe-16Cr-4Al-1Ni-0.4Y2O3 (% by weight) at 700, 800 and 900 °C, with exposure times of 5, 20, 50, and 100 h at each temperature. The purpose is to obtain new data on its high-temperature parabolic oxidation constant for assessing oxidation resistance. The methods used include isothermal oxidation testing, XRD, SEM-EDS characterization, and analysis of oxidation kinetics by monitoring changes in oxide thickness using microscopy and SEM-EDS. The oxide products formed on the sample surface are Fe2O3, Fe3O4, AlFe2O4, and (Fe,Cr)2O3. Al and Cr oxides are located under the dominant Fe oxide layer on the surface of the sample. The oxidation test results showed that the most protective sample was obtained at a temperature of 700 °C for 100 h with an oxide thickness of 263.99 μm. The kinetics analysis correlates strongly with the parabolic equation (R2 ≈ 1). The oxidation rate constants at temperatures of 700, 800, and 900 °C were 681.76, 2957.5, and 12300 μm2 h−1, respectively. The activation energy required by the oxidation reaction in this alloy is 136.5 kJ mol−1. This research enhances understanding and potential applications of the Fe-16Cr-4Al-1Ni-0.4Y2O3 alloy in high-temperature environments.