bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic Proctocolectomy for Ulcerative Colitis and Crohn’s Disease of the Large Intestine: Short-Term and Long-Term Outcomes of 53 Cases
Authors: Kucher M D , Bilianskyi L S , Kryvoruk M I , Tkachenko F H , Stelmakh A I 
Published: September 2016

One- or two-stage restorative total colectomy is recognized as surgery of choice when treating ulcerative colitis and Crohn’s disease of the large intestine. The possible advantages of laparoscopic approach are still being discussed. The objective of the research was to evaluate postoperative quality of life, short-term and long-term functional outcomes of 53 cases of laparoscopically assisted proctocolectomy for ulcerative colitis and Crohn’s disease of the large intestine in order to improve further application of laparoscopic approach. Materials and methods. Primary restorative proctocolectomy with transanal mucosectomy and the ileal pouch-anal anastomosis was performed in 5 patients with ulcerative colitis; total colectomy with low anterior resection of the rectum and double stapling ileal pouch-rectal anastomosis was performed in 8 patients; total proctocolectomy (with abdominoperineal resection of the rectum, terminal ileostomy) was performed in 3 patients with Crohn’s disease and multiple perianal fistulas; total colectomy, low anterior resection of the rectum, and terminal ileostomy (the first stage of surgical treatment) was performed in 37 patients; the second stage restorative surgery (J-pouch construction and the pouch-anal/low rectal anastomosing) was performed in 17 patients. The restorative procedure was followed by temporary diverting ileostomy in all patients.Results. The conversion to laparotomy was required in 3 (5.7%) cases. In one case during the second stage restorative surgery the urine bladder wall was damaged, and sutured laparoscopically. There was no postoperative mortality. The major complications of the early postoperative period included pelvic abscesses (4 patients), pouch-anal anastomosis leakage (1 patient), postoperative ileus (3 patients), and ileal pouch-perineal fistula (1patient). Stool frequency was about 6 times in a 24 hour period (4-11 times) 12 months after ileostomy closure. Pouchitis was observed in 4 patients. Pouch failure occurred in 1 patient due to severe pouchitis and anal incontinence.Conclusions. Laparoscopic proctocolectomy with extracorporeal ileal pouch construction, transanal mucosectomy, and pouch-anal anastomosis are considered as surgery of choice for ulcerative colitis. The pouch-rectal anastomosing is feasible for Crohn’s disease in individual cases. Total laparoscopic proctocolectomy with intracorporeal ileal pouch construction may be the next step in clinical trials.
Long-term Effect of Losartan on Kidney Disease in American Indians With Type 2 Diabetes: A Follow-up Analysis of a Randomized Clinical Trial
Authors: Tanamas Stephanie K. , Saulnier Pierre-Jean , Fufaa Gudeta D. , Wheelock Kevin M. , Weil E. Jennifer , Hanson Robert L. , Knowler William C. , Bennett Peter H. , Nelson Robert G. 
Published: September 2016

OBJECTIVE
To determine whether early administration of losartan slows progression of diabetic kidney disease over an extended period.

RESEARCH DESIGN AND METHODS
We conducted a 6-year clinical trial in 169 American Indians with type 2 diabetes and urine albumin/creatinine ratio &lt;300 mg/g; 84 participants were randomly assigned to receive losartan and 85 to placebo. Primary outcome was a decline in glomerular filtration rate (GFR; iothalamate) to ≤60 mL/min or to half the baseline value in persons who entered with GFR &lt;120 mL/min. At enrollment, GFR averaged 165 mL/min (interquartile range 49–313 mL/min). During the trial, nine persons reached the primary outcome with a hazard ratio (HR; losartan vs. placebo) of 0.50 (95% CI 0.12–1.99). Participants were then followed posttrial for up to 12 years, with treatment managed outside the study. The effect of losartan on the primary GFR outcome was then reanalyzed for the entire study period, including the clinical trial and posttrial follow-up.

RESULTS
After completion of the clinical trial, treatment with renin-angiotensin system inhibitors was equivalent in both groups. During a median of 13.5 years following randomization, 29 participants originally assigned to losartan and 35 to placebo reached the primary GFR outcome with an HR of 0.72 (95% CI 0.44–1.18).

CONCLUSIONS
Long-term risk of GFR decline was not significantly different between persons randomized to early treatment with losartan and those randomized to placebo. Accordingly, we found no evidence of an extended benefit of early losartan treatment on slowing GFR decline in persons with type 2 diabetes.
Diabetes Is Associated With Decreased Limb Survival in Patients With Critical Limb Ischemia: Pooled Data From Two Randomized Controlled Trials
Authors: Spreen Marlon I. , Gremmels Hendrik , Teraa Martin , Sprengers Ralf W. , Verhaar Marianne C. , Statius van Eps Randolph G. , de Vries Jean-Paul P.M. , Mali Willem P.Th.M. , van Overhagen Hans ,   on behalf of the PADI and JUVENTAS Study Groups
Published: September 2016

OBJECTIVE
Although never assessed prospectively, diabetes mellitus (DM) is assumed to negatively affect the outcomes of critical limb ischemia (CLI). DM was highly prevalent in two recently conducted randomized controlled trials in CLI patients, the PADI (Percutaneous Transluminal Balloon Angioplasty [PTA] and Drug Eluting Stents for Infrapopliteal Lesions in Critical Limb Ischemia) and JUVENTAS (Rejuvenating Endothelial Progenitor Cells via Transcutaneous Intra-Arterial Supplementation) trials. To determine the implications of DM in a population of patients with infrapopliteal CLI, clinical outcomes were compared in patients with and without DM.

RESEARCH DESIGN AND METHODS
Individual data from patients with CLI (Rutherford category ≥4) were pooled. Patients were considered to have DM when this diagnosis was reported in the hospital electronic medical records. Rates of major amputation (above ankle level) and major events (major amputation or death) were compared between CLI patients with and without DM. Hazard ratios (HRs) were calculated.

RESULTS
Of a total of 281 patients, DM was present in 49.1%. The major amputation rate at 5 years of follow-up was higher in patients with DM than in patients without DM (34.1% vs. 20.4%, P = 0.015). The major event and death rate did not differ. The unadjusted HR of DM for the major amputation risk was 1.87 (95% CI 1.12–3.12). Model factors with significant HRs in the multivariate analysis were baseline Rutherford category (HR 1.95; 95% CI 1.24–3.06) and ankle-brachial index (ABI) &gt;1.4 (HR 2.78; 95% CI 1.37–5.64).

CONCLUSIONS
CLI patients with DM are at a significantly higher risk of major amputation than CLI patients without DM. This increased risk is associated with a higher prevalence of baseline ABI &gt;1.4 and more severe ischemia at initial presentation in patients with DM.
Competition, salinity, and clonal growth in native and introduced irises
Authors: Mopper Susan , Wiens Karen C. , Goranova Greta A. 
Published: September 2016

PREMISE OF THE STUDY:Iris pseudacorusspread rapidly into North America after introduction from Europe in the 1800s and now co‐occurs with nativeI. hexagonain freshwater Louisiana wetlands. Native irises support and interact with multiple trophic levels, whereasI. pseudacorusis classified an invasive pest because it grows aggressively, reduces biodiversity, and displaces native vegetation. Salinity levels are increasing in coastal wetlands worldwide. We examined how salt‐stress affects competitive interactions between these conspecifics.METHODS:We established a three‐way full‐factorial common‐garden experiment that included species (I. pseudacorus, I. hexagona), competition (no competition, intraspecific competition, and interspecific competition), and salinity (0, 4, 8 parts per thousand NaCl), with six replicates per treatment.KEY RESULTS:After 18 mo,Iris pseudacorusproduced much more biomass than the native species did (F1, 92= 71.5,P< 0.0001). Interspecific competition did not affect the introduced iris, but biomass of the native was strongly reduced (competition × species interaction:F2, 95= 76.7,P= 0.002). Salinity significantly reduced biomass of both species (F2, 92= 21.8,P< 0.0001), with no species × salinity interaction (F2, 84= 1.85,P= 0.16).CONCLUSIONS:Our results demonstrate that salt stress strongly reduced clonal reproduction in native and introduced irises; however, the introduced iris had a competitive advantage over the native, regardless of environmental salinity levels. Based on patterns in clonal reproduction, the introduced iris could potentially threaten native iris populations. We are currently investigating seed production and mortality during competition and stress because both clonal and sexual reproduction must be considered when predicting long‐term population dynamics.
La chronique au Mexique : évolution et perspectives
Authors: Léziart Françoise 
Published: September 2016

Partant des premières chroniques journalistiques du xixe siècle, frivoles, patriotiques ou déjà combatives pour ensuite aborder les textes d’Elena Poniatowska et de Carlos Monsiváis (les chroniqueurs emblématiques du Mexique des années 1960-1990) notre travail cherche à souligner l’évolution (tant thématique que rhétorique) de ce type d’expression hybride dont on trouve certains prolongements dans les ouvrages d’un auteur comme Jorge Volpi. Cela nous amène à réfléchir également au rôle du chroniqueur et aux conditions spécifiques de production de ce type de texte.
Estampas de ocio, buen humor y reflexión : les chroniques de l’écrivain péruvien Edgardo Rivera Martínez
Authors: Aubès Françoise 
Published: September 2016

Edgardo Rivera Martínez (Jauja 1933), nouvelliste, romancier, qui a connu un certain succès en 1993 avec País de Jauja, roman du métissage heureux, est aussi l’auteur d’une œuvre journalistique réunie dans plusieurs recueils dont celui qui donne son titre à cet article. Depuis 1976, l’écrivain publie de façon régulière dans le supplément dominical de divers quotidiens liméniens des « estampas », sur des sujets très variés comme l’emploi excessif de la locution o sea que par les jeunes Liméniennes qu’il surnommera les « Oseanidas », jusqu’aux souvenirs de la Lima d’autrefois. Ajoutons à cela ses « Estampas de viaje », chroniques de ses voyages dans les années 1950 et 1960 en Europe (Al andar de los caminos), sans oublier A la hora de la tarde y de los juegos (l’enfance à Jauja). Edgardo Rivera Martínez est un observateur amène, parfois complice parfois moraliste, jamais caustique ni racoleur.Cet article étudie la place que tiennent ces chroniques dans l’œuvre de l’écrivain.
Mexico D.F. au crible des chroniques
Authors: Olivier Florence 
Published: September 2016

Cet article examine et compare l’anthologie chorale de chroniques urbaines México D.F.: Lecturas para paseantes (2005) de Rubén Gallo et le dernier livre que Carlos Monsiváis a consacré à la culture urbaine de la capitale mexicaine : Apocalipstick (2009). Les deux ouvrages s’intéressant à la ville qui « émerge des ruines de la dernière modernité », il ressort de leur lecture croisée un état des lieux de la capitale, plus directement marqué par une vision politique chez Monsiváis, exaltant, comme dans l’essai qu’écrit Rubén Gallo en introduction de son volume, la paradoxale vitalité de ces lieux si mouvants. Les chroniqueurs littéraires de la capitale fédérale en explorent les spectaculaires métamorphoses, y sondent les expressions de la culture populaire, narrent les fonctions politiques de l’espace public, mettent la polis en mots et en scène. Tout un registre d’écritures de la ville apparaît ainsi, qui rend compte de la nature polymorphe du genre de la chronique.
« Une teste de cire anatomique »
Authors: Taddia Elena 
Published: September 2016

Cette étude retrace la vie de Gaetano Giulio Zumbo (1656 ?-1701), sculpteur sur cire et céroplaste sicilien mort à Paris le 22 décembre 1701, un an à peine après son arrivée dans la capitale française. Pourquoi Zumbo arrive-t-il à Paris, quels réseaux lui permettent d’être reçu à la cour, d’obtenir un privilège royal, et comment son œuvre louée, discréditée et reléguée au rang d’« art mineur », a-t-elle pourtant influencé la sculpture et l’anatomie ? Telles sont les questions que cette étude se propose d’aborder, qui mettent en évidence un aspect important de la vie de Zumbo : celui de l’artiste de cour intermittent. Il séjourna d’abord à Florence chez Côme III de Médicis, avant d’être admis à la cour de Monseigneur et du duc d’Orléans, protégé par la peintre Élisabeth Chéron et admiré par les hommes de sciences et les artistes du Paris de l’époque.
De nieuwe Handreiking beleidsdoorlichtingen
Authors: ten Cate Birgit , Israël Erik 
Published: September 2016

De nieuwe Handreiking beleidsdoorlichtingen
Het Rijk evalueert in principe elk beleidsterrein eens in de zeven jaar door middel van een beleidsdoorlichting. Dit is niet eenvoudig. De Handreiking beleidsdoorlichtingen biedt hierbij in de vorm van vele praktische tips de helpende hand.
Direct Isolation and Characterization of Human Nephron Progenitors
Authors: Da Sacco Stefano , Thornton Matthew E. , Petrosyan Astgik , Lavarreda-Pearce Maria , Sedrakyan Sargis , Grubbs Brendan H. , De Filippo Roger E. , Perin Laura 
Published: September 2016

Abstract
Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures. With the use of RNA-labeling probes, we isolated SIX2+CITED1+ cells from human fetal kidney for the first time. We confirmed their nephrogenic state by gene profiling and evaluated their nephrogenic capabilities in giving rise to mature renal cells. We also evaluated the ability to culture these cells without complete loss of SIX2 and CITED1 expression over time. In addition to defining the gene profile of human NPs, this in vitro system facilitates studies of human renal development and provides a novel tool for renal regeneration and bioengineering purposes.
Are Mesenchymal Stem Cells So Bloody Great After All?
Authors: Marfy-Smith Stephanie J. , Clarkin Claire E. 
Published: September 2016

Abstract
This Perspective discusses some activities of mesenchymal stem cells (MSCs) in the context of angiogenesis, focusing on contrasting effects that could call into question the extent to which MSCs can be used clinically in the future. We report on the antiangiogenic/antiproliferative effects of specific MSC populations (including bone marrow MSCs), their paracrine activity, tissue heterogeneity, and endothelial cell interactions. Also discussed are what could lead to contrasting effects of the influence of MSCs in regulating angiogenesis, pointing to some negative effects of these cells. In conclusion, this article highlights important aspects of MSC behavior within the perspective of translational medicine applications.
Stem Cell-Based Tissue-Engineered Laryngeal Replacement
Authors: Ansari Tahera , Lange Peggy , Southgate Aaron , Greco Karin , Carvalho Carla , Partington Leanne , Bullock Anthony , MacNeil Sheila , Lowdell Mark W. , Sibbons Paul D. , Birchall Martin A. 
Published: September 2016

AbstractPatients with laryngeal disorders may have severe morbidity relating to swallowing, vocalization, and respiratory function, for which conventional therapies are suboptimal. A tissue-engineered approach would aim to restore the vocal folds and maintain respiratory function while limiting the extent of scarring in the regenerated tissue. Under Good Laboratory Practice conditions, we decellularized porcine larynges, using detergents and enzymes under negative pressure to produce an acellular scaffold comprising cartilage, muscle, and mucosa. To assess safety and functionality before clinical trials, a decellularized hemilarynx seeded with human bone marrow-derived mesenchymal stem cells and a tissue-engineered oral mucosal sheet was implanted orthotopically into six pigs. The seeded grafts were left in situ for 6 months and assessed using computed tomography imaging, bronchoscopy, and mucosal brushings, together with vocal recording and histological analysis on explantation. The graft caused no adverse respiratory function, nor did it impact swallowing or vocalization. Rudimentary vocal folds covered by contiguous epithelium were easily identifiable. In conclusion, the proposed tissue-engineered approach represents a viable alternative treatment for laryngeal defects.
Enhanced Expansion and Sustained Inductive Function of Skin-Derived Precursor Cells in Computer-Controlled Stirred Suspension Bioreactors
Authors: Agabalyan Natacha A. , Borys Breanna S. , Sparks Holly D. , Boon Kathryn , Raharjo Eko W. , Abbasi Sepideh , Kallos Michael S. , Biernaskie Jeff 
Published: September 2016

AbstractEndogenous dermal stem cells (DSCs) reside in the adult hair follicle mesenchyme and can be isolated and grown in vitro as self-renewing colonies called skin-derived precursors (SKPs). Following transplantation into skin, SKPs can generate new dermis and reconstitute the dermal papilla and connective tissue sheath, suggesting they could have important therapeutic value for the treatment of skin disease (alopecia) or injury. Controlled cell culture processes must be developed to efficiently and safely generate sufficient stem cell numbers for clinical use. Compared with static culture, stirred-suspension bioreactors generated fivefold greater expansion of viable SKPs. SKPs from each condition were able to repopulate the dermal stem cell niche within established hair follicles. Both conditions were also capable of inducing de novo hair follicle formation and exhibited bipotency, reconstituting the dermal papilla and connective tissue sheath, although the efficiency was significantly reduced in bioreactor-expanded SKPs compared with static conditions. We conclude that automated bioreactor processing could be used to efficiently generate large numbers of autologous DSCs while maintaining their inherent regenerative function.
Cartilage Regeneration in Osteoarthritic Patients by a Composite of Allogeneic Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Hyaluronate Hydrogel: Results from a Clinical Trial for Safety and Proof-of-Concept with 7 Years of Extended Follow-Up
Authors: Park Yong-Beom , Ha Chul-Won , Lee Choong-Hee , Yoon Young Cheol , Park Yong-Geun 
Published: September 2016

Abstract
Few methods are available to regenerate articular cartilage defects in patients with osteoarthritis. We aimed to assess the safety and efficacy of articular cartilage regeneration by a novel medicinal product composed of allogeneic human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). Patients with Kellgren-Lawrence grade 3 osteoarthritis and International Cartilage Repair Society (ICRS) grade 4 cartilage defects were enrolled in this clinical trial. The stem cell-based medicinal product (a composite of culture-expanded allogeneic hUCB-MSCs and hyaluronic acid hydrogel [Cartistem]) was applied to the lesion site. Safety was assessed by the World Health Organization common toxicity criteria. The primary efficacy outcome was ICRS cartilage repair assessed by arthroscopy at 12 weeks. The secondary efficacy outcome was visual analog scale (VAS) score for pain on walking. During a 7-year extended follow-up, we evaluated safety, VAS score, International Knee Documentation Committee (IKDC) subjective score, magnetic resonance imaging (MRI) findings, and histological evaluations. Seven participants were enrolled. Maturing repair tissue was observed at the 12-week arthroscopic evaluation. The VAS and IKDC scores were improved at 24 weeks. The improved clinical outcomes were stable over 7 years of follow-up. The histological findings at 1 year showed hyaline-like cartilage. MRI at 3 years showed persistence of the regenerated cartilage. Only five mild to moderate treatment-emergent adverse events were observed. There were no cases of osteogenesis or tumorigenesis over 7 years. The application of this novel stem cell-based medicinal product appears to be safe and effective for the regeneration of durable articular cartilage in osteoarthritic knees.
Multimodal Delivery of Isogenic Mesenchymal Stem Cells Yields Synergistic Protection from Retinal Degeneration and Vision Loss
Authors: Bakondi Benjamin , Girman Sergey , Lu Bin , Wang Shaomei 
Published: September 2016

Abstract
We previously demonstrated that subretinal injection (SRI) of isogenic mesenchymal stem cells (MSCs) reduced the severity of retinal degeneration in Royal College of Surgeons rats in a focal manner. In contrast, intravenous MSC infusion (MSCIV) produced panoptic retinal rescue. By combining these treatments, we now show that MSCIV supplementation potentiates the MSCSRI-mediated rescue of photoreceptors and visual function. Electrophysiological recording from superior colliculi revealed 3.9-fold lower luminance threshold responses (LTRs) and 22% larger functional rescue area from combined treatment compared with MSCSRI alone. MSCIV supplementation of sham (saline) injection also improved LTRs 3.4-fold and enlarged rescue areas by 27% compared with saline alone. We confirmed the involvement of MSC chemotaxis for vision rescue by modulating C-X-C chemokine receptor 4 activity before MSCIV but without increased retinal homing. Rather, circulating platelets and lymphocytes were reduced 3 and 7 days after MSCIV, respectively. We demonstrated MSCSRI-mediated paracrine support of vision rescue by SRI of concentrated MSC-conditioned medium and assessed function by electroretinography and optokinetic response. MSC-secreted peptides increased retinal pigment epithelium (RPE) metabolic activity and clearance of photoreceptor outer segments ex vivo, which was partially abrogated by antibody blockade of trophic factors in concentrated MSC-conditioned medium, or their cognate receptors on RPE. These data support multimodal mechanisms for MSC-mediated retinal protection that differ by administration route and synergize when combined. Thus, using MSCIV as adjuvant therapy might improve cell therapies for retinal dystrophy and warrants further translational evaluation.
Subcircular conduits and dikes offshore the Somma‐Vesuvius volcano revealed by magnetic and seismic data
Authors: Paoletti V. , Passaro S. , Fedi M. , Marino C. , Tamburrino S. , Ventura G. 
Published: September 2016

AbstractWe analyzed new magnetic, bathymetric, and seismic data acquired in the offshore sector of Somma‐Vesuvius volcano (Italy). We detected a group of high‐intensity, short wavelength magnetic anomalies corresponding to partly buried volcanic dome‐like structures located by seismic data. The magnetic anomalies are aligned along a NW‐SE strike that is the preferential orientation of an eruptive fracture of the pre‐19 ka activity of Vesuvius. Four cones emplaced after the Last Glacial Maximum (19 ka), whereas a fifth one emplaced more recently suggesting a rejuvenation of the eruptive system offshore the volcano in historical times. We also identified a NE‐SW elongated magnetic anomaly consistent with a dike‐like body associated to an on‐land tectonic structure that was active in recent times at Vesuvius. A delta‐like area with diffuse low‐intensity magnetic anomalies reflects the seaward fronts of lava flows that entered the sea mainly during the Middle Ages.