bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Organizational Resources on Organizational Agility in Hospitality Industry: A Dynamic Capabilities Approach
Authors: Chasapi Panagiota , Pateli Adamantia , Mylonas Naoum , Kourouthanassis Panagiotis 
Published: October 2024

ABSTRACTThe present study investigates the relationship between organizational resources (financial, social, and technological capital) and dynamic capabilities development in crises periods. Through quantitative research, a theoretical framework that is based on resource‐based view and dynamic capabilities view is validated for the first time in the hospitality industry. In practice, it is explored whether the hospitality firms—based on their own resources—can develop the dynamic capability of organizational agility that enables them to cope with turbulent and unstable environments. The empirical research was conducted with the sample of 502 hospitality firms in Greece during the COVID‐19 era. The findings confirm that financial and technological capital have a positive influence on the development of agility‐related dynamic capabilities, and specifically market capitalizing agility and operational adjustment agility, for the hospitality industry during turbulent periods. Instead, social capital has a negative and no effect respectively on these two dynamic capabilities.
Variation Patterns and Affecting Factors of Plant Alpha Diversity, Beta Diversity and Its Components in Restoration Grasslands on Loess Plateau
Authors: Jian Chunxia , Luo Yang , Zhou Junjie , Xu Bingcheng 
Published: October 2024

ABSTRACTUnderstanding spatiotemporal variation in diversity and identifying key external affecting factors are essential for biodiversity conservation. However, community assembly and species diversity in natural grassland (NG) restoration on the Loess Plateau remain unclear. In this study, we examined α diversity (species richness), β diversity (βtotal), and its components (βrepl and βrich) across 89 grassland communities, which were categorized into five restoration stages: recent grassland (RG), early restoration (ER), middle restoration (MR), later restoration (LR), and NG. Linear mixed models (LMM) were used to analyze the effects of topographic, soil, and landscape factors on diversity. Results showed that α diversity followed an increasing‐decreasing trend across restoration stages (7–21 species m−2), peaking at the MR stage. In contrast, β diversity and its two components declined with duration, with βtotal and βrepl being notably higher in ER than in LR and NG. Differences in βtotal primarily resulted from species replacement (βrepl, 61%–70%), with a smaller contribution from species gain and loss (βrich, 30%–39%). The LMM also revealed that restoration stage was the most important factor affecting plant diversity, explaining 59.3%, 68.4%, 53.5%, and 58.8% of richness, βtotal, βrepl, and βrich, respectively. In comparison, landscape had weaker effects on diversity (17.2%, 24.2%, 46.5%, 39.8%), while topography and soil factors had the least effects. In summary, deterministic processes (restoration stage) dominate natural restoration, but substantial differences persist between restoration grassland and NG. This study provides valuable insights for assessing restoration progress, prioritizing conservation areas, and informing future grassland management in semi‐arid and fragmented regions.
Strong large‐scale structure–function coupling in benthic bacteria is mediated by algae in a geodiverse river network
Authors: Thuile Bistarelli Lukas , Fuß Thomas , Walther Franziska , Zoccarato Luca , Talluto Lauren , Romaní Anna M. , Grossart Hans‐Peter , Singer Gabriel A. 
Published: October 2024

AbstractBenthic bacteria in stream ecosystems drive organic matter mineralization. However, knowledge on how this ecosystem function is driven by bacterial community composition in interaction with environmental conditions and organic matter resources is poor. This is especially true when considering the regional scale of river networks, at which environmental conditions vary in a scale‐dependent manner and are spatially structured due to asymmetrical water flow. Similarly, organic matter resources may have a terrestrial origin in remote headwaters or be sourced locally from algae living in close proximity to bacteria in benthic biofilms. We investigated benthic biofilm meta‐community structure and function across the > 6700 km2 river network of the near‐natural Vjosa in Albania and Greece and found a strong control of the benthic algal community on bacterial community composition (13.4% of variability explained). In addition, bacterial community composition has linkages to water chemistry, which itself is strongly shaped by the diverse geology in the catchment, and to dispersal, shaping metacommunity structure as a neutral process. Notably, bacterial community composition explained the largest single fraction of variability (31.5%) in extracellular enzymatic activities, while there was no dependency of enzyme ratios on organic matter nor environmental conditions. Synergistic effects between bacteria and algae accounted for additional 47.3% of variability in heterotrophic functioning, emphasizing the importance of algal–bacterial interactions in benthic biofilms. Our findings shed new light on bacterial structure–function coupling highlighting the importance of algal–bacterial interactions at the river network scale.
Methylation, Gene Expression, and Risk Genotypes at the TERT‐CLPTM1L Locus in Cervical Cancer
Authors: Ramachandran Dhanya , Mao Qianqian , Liao Dandan , Kamal Maud , Schürmann Peter , Eisenblätter Rieke , Geffers Robert , Balint Balazs , Lecompte Lolita , Servant Nicolas , Chérif Linda Larbi , Lamy Constance , Baulande Sylvain , Legoix Patricia , Le Tourneau Christophe , Latouche Aurélien , Hillemanns Peter , Scholl Suzy , Dörk Thilo 
Published: October 2024

ABSTRACTThe reverse transcriptase subunit of telomerase, TERT, is frequently activated in high‐grade dysplasia and invasive cancers of the uterine cervix. Telomerase activation through hypomethylation of the TERT promoter holds promise as a biomarker for cervical cancer progression, however, specific CpG sites involved in cervical cancer risk remain to be fully defined. A recent genome‐wide association study on cervical cancer identified genetic polymorphisms at 5p13.33 (close to TERT‐CLPTM1L) but the underlying mechanisms are undetermined. We investigated 529 CpG sites within the TERT promoter region and 3 CpG islands nearby, and 21 CpG sites within CLPTM1L in 190 bisulfite‐converted cervical tumor DNA samples from BioRAIDs (NCT02428842). We identified eight CpG sites within TERT intron 2 where methylation was significantly associated with the genotypes of cervical cancer risk variants rs27070 and rs459961 in cervical tumors after multiple testing correction (p < 9.4 × 10E−5). Hypermethylation at chr5:1289663 correlated with decreased TERT mRNA levels. In an independent series of 188 normal or dysplastic cervical tissues, rare alleles of rs27070 and rs459961 were associated with low basal CLPTM1L levels and with the absence of TERT mRNA in HPV‐negative samples, consistent with their proposed role as protective variants for cervical cancer. HPV infection was associated with increased CLPTM1L and TERT levels. Collectively, our results provide a link between cervical cancer risk variants, methylation, and gene expression and implicate both TERT and CLPTM1L as genes modulated by genomic background and HPV infection during cervical cancer development.
Deep Sequencing and Phenotyping in an Australian Tuberous Sclerosis Complex “No Mutations Identified” Cohort
Authors: Chung Clara W. T. , Bournazos Adam M. , Chan Lok Chi Denise , Sarkozy Vanessa , Lawson John , Kennedy Sean E. , Cooper Sandra T. , Kirk Edwin P. , Mowat David 
Published: October 2024

ABSTRACTTuberous sclerosis complex (TSC) is a variable multisystem disorder. The “no mutations identified” (NMI) group are reportedly phenotypically milder than those with an identified molecular cause, and often have mosaic or intronic variants not detected by standard sequencing methods.MethodsWe describe the phenotypes in an Australian TSC NMI group (n = 18) and a molecular testing strategy implementable in a diagnostic laboratory. Massively parallel sequencing (MPS) of the whole genomic regions of TSC1 and TSC2 was performed using DNA extracted from multiple tissue samples per participant.ResultsOur study showed that the phenotype in TSC NMI individuals can be similar to those with heterozygous, particularly TSC1, variants. Although neurodevelopmental outcomes can be less severe, the number of organ systems involved was similar to the non‐mosaic groups. A diagnostic yield of 72% (13/18) was achieved, with the majority (10/13) being mosaic variants and the remainder heterozygous variants missed on previous testing.ConclusionTesting DNA from multiple tissue samples allowed for validation of otherwise discarded low‐level mosaic variants and detection of mosaic variants by MPS without excessive cost or the need for specialised techniques. Implementing this approach in a diagnostic setting is viable and allows optimal clinical care of patients with NMI TSC.
Staphylococcus aureus SOS response: Activation, impact, and drug targets
Authors: Cheng Kaiying , Sun Yukang , Yu Huan , Hu Yingxuan , He Yini , Shen Yuanyuan 
Published: October 2024

AbstractStaphylococcus aureus is a common cause of diverse infections, ranging from superficial to invasive, affecting both humans and animals. The widespread use of antibiotics in clinical treatments has led to the emergence of antibiotic‐resistant strains and small colony variants. This surge presents a significant challenge in eliminating infections and undermines the efficacy of available treatments. The bacterial Save Our Souls (SOS) response, triggered by genotoxic stressors, encompasses host immune defenses and antibiotics, playing a crucial role in bacterial survival, invasiveness, virulence, and drug resistance. Accumulating evidence underscores the pivotal role of the SOS response system in the pathogenicity of S. aureus. Inhibiting this system offers a promising approach for effective bactericidal treatments and curbing the evolution of antimicrobial resistance. Here, we provide a comprehensive review of the activation, impact, and key proteins associated with the SOS response in S. aureus. Additionally, perspectives on therapeutic strategies targeting the SOS response for S. aureus, both individually and in combination with traditional antibiotics are proposed.
Elliptical Halbach magnet and gradient modules for low‐field portable magnetic resonance imaging
Authors: Galve Fernando , Pallás Eduardo , Guallart‐Naval Teresa , García‐Cristóbal Pablo , Martínez Pablo , Algarín José M. , Borreguero Jose , Bosch Rubén , Juan‐Lloris Francisco , Benlloch José M. , Alonso Joseba 
Published: October 2024

AbstractThis study aims to develop methods to design the complete magnetic system for a truly portable MRI scanner for neurological and musculoskeletal (MSK) applications, optimized for field homogeneity, field of view (FoV), and gradient performance compared to existing low‐weight configurations. We explore optimal elliptic‐bore Halbach configurations based on discrete arrays of permanent magnets. In this way, we seek to improve the field homogeneity and remove constraints to the extent of the gradient coils typical of Halbach magnets. Specifically, we have optimized a tightly packed distribution of magnetic Nd2Fe14B cubes with differential evolution algorithms and a second array of shimming magnets with interior point and differential evolution methods. We have also designed and constructed an elliptical set of gradient coils that extend over the whole magnet length, maximizing the distance between the lobe centers. These are optimized with a target field method minimizing a cost function that considers also heat dissipation. We have employed the new toolbox to build the main magnet and gradient modules for a portable MRI scanner designed for point‐of‐care and residential use. The elliptical Halbach bore has semi‐axes of 10 and 14& cm, and the magnet generates a field of 87& mT homogeneous down to 5700& ppm (parts per million) in a 20‐cm diameter FoV; it weighs 216& kg and has a width of 65& cm and a height of 72& cm. Gradient efficiencies go up to around 0.8& mT/m/A, for a maximum of 12& mT/m within 0.5& ms with 15& A and 15& V amplifier. The distance between lobes is 28& cm, significantly increased with respect to other Halbach‐based scanners. Heat dissipation is around 25& W at maximum power, and gradient deviations from linearity are below 20% in a 20‐cm sphere.Elliptic‐bore Halbach magnets enhance the ergonomicity and field distribution of low‐cost portable MRI scanners, while allowing for full‐length gradient support to increase the FoV. This geometry can be potentially adapted for a prospective low‐cost whole‐body technology.
Critical soft skill competencies that clinical nurse educators consider important to evaluate in nurses
Authors: Song Youngkwan , Lafond Cynthia M. , Vincent Catherine , Kim Mi Ja , Park Chang G. , McCreary Linda L. 
Published: October 2024

AbstractAimGuided by Benner's framework, From Novice to Expert, this study aimed to identify (1) critical soft skills to be evaluated in nurses and (2) levels of nursing behaviour indicating achievement of soft skills to provide a framework for developing a soft skills rubric.Background/IntroductionNurse shortages are often attributable to high turnover rates among nurses. To improve this situation, healthcare facilities implement transition programmes and continuing education with a primary focus on developing and maintaining nurses' knowledge and performance (hard skills). However, balancing hard and soft skills (beliefs, values and attitudes) is key to nurses' quality of care and ultimately to their retention. Despite the importance of soft skills, their intangible nature creates problems in evaluating nurses' attainment of these skills and in providing constructive feedback to help them set concrete goals for improving their practice.MethodsThis qualitative descriptive study described critical soft skills in the nursing context. A purposeful sampling strategy was used to enrol 13 clinical nurse educators from multiple hospital units; each had more than 2 years of clinical nurse educator experience and had completed preceptor training. One‐to‐one interviews with these individuals were audio recorded, transcribed and subjected to direct content analysis using NVivo 12. The study follows the Consolidated criteria for reporting qualitative research (COREQ) guidelines for qualitative studies.FindingsFive main competencies, comprising 20 subcompetencies, were identified: personal growth, effective interactions, professionalism, teamwork and the caring role. For each subcompetency, four skill levels were delineated using clinical indicators.ConclusionThis study's findings can enhance understanding of clinical nurse educators' perceptions regarding soft skills required of nurses.Implications for the profession and/or patient careThe soft skills identified can be incorporated into a rubric to be used by clinical nurse educators to evaluate and guide nurses' professional development and contribute to improving quality of care.No patient or public contribution is required for this study.
Potential differences in receptor‐mediated G‐protein activation in postmortem human hippocampal membranes prepared from healthy controls and suicide victims
Authors: Odagaki Yuji , Kinoshita Masakazu , Palkovits Miklós , Borroto‐Escuela Dasiel Oscar , Fuxe Kjell 
Published: October 2024

AbstractAimPostmortem brain studies offer enormous opportunities to study molecular mechanisms associated with suicide. In the present study, conventional [35S]GTPγS binding assay and its version‐up method ([35S]GTPγS binding/immunoprecipitation assay) were applied to postmortem human hippocampal membranes prepared from suicide victims and control subjects.MethodsBy using conventional [35S]GTPγS binding assay, functional activations of Gi/o proteins coupled with multiple GPCRs (5‐HT1A receptor, α2A‐adrenoceptor, M2/M4 mAChRs, adenosine A1 receptor, histamine H3 receptor, group II mGlu, GABAB receptor, μ‐opioid receptor, δ‐opioid receptor, and NOP receptor) were detected by using 15 different agonists. Furthermore, 5‐HT2A receptor‐ and M1 mAChR‐mediated Gαq/11 activation and adenosine A1 receptor‐mediated Gαi‐3 activation were detectable by means of [35S]GTPγS binding/immunoprecipitation assay.ResultsNo significant differences in pharmacological parameters of all concentration‐response curves investigated were found between suicide victims and control subjects. Significant correlations were obtained for the maximal percent increases between some distinct signaling pathways.ConclusionAlthough only preliminary and auxiliary results were obtained as to the potential differences between suicide victims and control subjects because of the limited number of subjects as well as unmatched age and postmortem delay, adenosine A1 receptor‐mediated Gαi/o activation and 5‐HT2A receptor‐mediated Gαq/11 activation appear worth focusing on in the future investigations. This study also indicates the possibility that some distinct signaling pathways are interrelated with each other, for example, functional activations of Gi/o proteins coupled to M2/M4 mAChR and 5‐HT1A receptor, NOP receptor, and GABAB receptor, and NOP receptor and δ‐opioid receptor.
Farmer identities and permanent grassland management: Evidence from five European biogeographic zones
Authors: Jin Shan , Cao Yiying , Burd Michael , Tindale Sophie , Feng Zhiming , Green Olivia , Newell‐Price Paul , Vicario‐Modroño Victoria , Mack Gabriele , Sánchez‐Zamora Pedro , Gallardo‐Cobos Rosa , Spörri Martina , El Benni Nadja , Alonso Natasha , Miškolci Simona , Outhwaite Samantha , Hunter Erik , Frewer Lynn J. 
Published: October 2024

Abstract

Permanent grassland (PG) provides multiple ecosystem services. However, there is increasing concern regarding the decreased multifunctionality of PGs, including those located in Europe. The decreased PG multifunctionality has been attributed to the increased intensity of PG management, where decision‐making is influenced by farmers' relevant behavioural intentions and self‐identities.

In order to investigate how farmer identities can translate into future adoption of PG management practices, interviews (n = 373) were conducted with farmers from five European biogeographic zones. Their farms are located in Continental/Pannonian (Czech Republic), Mediterranean (Spain), Boreal (Sweden), Alpine (Switzerland) and Atlantic (United Kingdom) biogeographic regions. The data were analysed using a mixed‐method approach involving thematic analysis and multinomial logistic regression.

The thematic analysis enabled seven farmer ‘identity’ types to be identified. The results of multinomial logistic regression showed that productivist identity was a predictor of farmers' intention to intensify or extensify PG in future, while land caretaker identity predicted maintenance of current PG management practice. Farmers with a higher dependence on income from agri‐environment schemes were more likely to extensify their PG in future, while those with a higher dependence on income from farm production were more likely to maintain current PG management practices. Older farmers were less likely to extensify their PG, and those having organic and extensive farms were more likely to intensify PG in future.

Future policies and interventions that aim to increase PG‐related ecosystem‐service multifunctionality can be more targeted by considering specific farm attributes, farmer identities and their future PG management intentions, as well as regional differences in these. Financial benefits and risks perceived by farmers should be considered when promoting any management practices, including policy interventions and policy levers.

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A Preliminary Phenomenological Exploration of Experiences of the Empty Pelvis Syndrome Derived From a Modified‐Delphi: The Price of Survival Following Pelvic Exenteration for Advanced Pelvic Cancer
Authors: West Charles T. , Denys Andreas , Rose Sam A. , Pape Eva , van Ramshorst Gabrielle H. , Sutton Paul A. , Yano Hideaki , West Malcolm A. , Mirnezami Alex H. , Calman Lynn , Sodergren Samantha C. 
Published: October 2024

ABSTRACTObjectiveThe empty pelvis syndrome (EPS) is common after pelvic exenteration (PE), causing fluid collections, bowel obstruction, perineal sinuses, and fistulas. The best approach to fill the pelvis to mitigate this remains controversial, and the impact of EPS on health‐related quality of life (HrQoL) is unknown. This study is the first to begin to explore lived‐experiences of EPS complications.MethodsUnstructured EPS virtual focus group meetings were conducted with a convenience sample of patients who underwent PE, as an extension of a modified‐Delphi study. Interpretative phenomenological analysis was conducted on verbatim transcripts to generate group experiential themes.ResultsTwelve patients (eight UK, one Dutch, and three Belgian) participated in four focus groups. Eight EPS complications were reported, (two pelvic collections, five chronic perineal sinuses, and one bowel obstruction). Group experiential themes were ‘Out of Options’, depicting patients forced to accept complications or limited survival; ‘The New Normal’, with EPS potentially delaying adaptation to post‐PE HrQoL; ‘Information Influencing Adaptation,’ emphasising the significance of patients understanding EPS to cope with its effects; and ‘Symptoms,’ reporting manifestations of EPS, the resultant physical limitations, and an intangible feeling that patients lost part of themselves.ConclusionsEPS may influence patient decision‐making, regret, adaptation, and information‐seeking. It can cause a variety of unpleasant symptoms and physical limitations, which may include phantom phenomenon. This work supports ongoing purposeful HrQoL research to better define these themes.
Interplays Between Matrix Metalloproteinases and Neurotropic Viruses: An Overview
Authors: Alghamdi Suad A. , Alissa Mohammed , Alghamdi Abdullah , Alshehri Mohammed A. , Albelasi Abdullah , Alzahrani Khalid J. , Safhi Awaji Y. 
Published: October 2024

ABSTRACTMatrix metalloproteinases (MMPs) are a diverse group of proteases involved in various physiological and pathological processes through modulation of extracellular matrix (ECM) components, cytokines, and growth factors. In the central nervous system (CNS), MMPs play a major role in CNS development, plasticity, repair, and reorganisation contributing to learning, memory, and neuroimmune response to injury. MMPs are also linked to various neurological disorders such as Alzheimer's disease, Parkinson's disease, cerebral aneurysm, stroke, epilepsy, multiple sclerosis, and brain cancer suggesting these proteases as key regulatory factors in the nervous system. Moreover, MMPs have been involved in the pathogenesis of neurotropic viral infections via dysregulation of various cellular processes, which may highlight these factors as potential targets for the treatment and control of neurological complications associated with viral pathogens. This review provides an overview of the roles of MMPs in various physiological processes of the CNS and their interactions with neurotropic viral pathogens.