bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the Efficacy and Characterization of Plantago major Extract Microspheres for Novel drug Delivery
Authors: Nongsiej Banshaiborlang , Roy Probin Kr , . Lalruatsangi , . Laldinchhana , Lalhlenmawia H , Das Sonjit , Sawant Sanjay D. 
Published: October 2024

Abstract

Background
In the recent years, advancement in novel drug delivery system with new technologies have emerged to deliver plant bioactives and extracts. The delivery system offers enhanced efficacy, better compatibility and enhanced shelf life and stability of the product. The current study aimed to formulate microspheres containing Plantago major extract and its evaluation and characterization.
Methods
Extract loaded microspheres were prepared using the solvent evaporation technique and were characterized using sophisticated tools like FTIR, DSC, SEM etc.
Results
Phytochemical screening of PME confirmed presence of compounds like alkaloids, flavonoids and phenols etc. Total phenolic and flavonoid contents were found to be 126 mg GAE/g and 312.54 mg QE/g respectively. LCMS analysis confirmed the presence of bioactive compounds including luteolin, baicalein etc. SEM revealed spherical and porous microspheres and DSC/FTIR confirmed PME-polymer compatibility. Entrapment efficiency ranged from 7.211–19.687% and drug release was negligible in an acidic environment, sustained alkaline media. F1 with low polymer, content release was 64% and F5 with highest polymer, content release was 41% over 12 hours. The release profile of the microspheres involves polymer relaxation and erosion along with drug diffusion
Conclusion
PME-loaded microspheres show potential for sustained release of contents for inflammation.
Lawsonia inermis (Henna) Extracts as a Sustainable Solution to Combat Multidrug-Resistant Gram-Negative Bacteria in Surface Water, Sewage, and Abattoir Leachate: A Quantitative Antimicrobial Analysis
Authors: Olaitan Morufat Oluwatosin , Nwadike Blessing Ifeoma 
Published: October 2024

Abstract

Antimicrobial resistance (AMR) poses a critical global public health threat, exacerbated by the presence of multidrug-resistant (MDR) bacteria in environmental matrices, particularly in Low- and Middle-Income Countries (LMICs). This study investigated the potential of Lawsonia inermis (Henna) extracts to inhibit MDR bacteria in surface water, sewage, and abattoir leachate. Using cold maceration extraction method, and methanol and aqueous as solvent, the antibacterial activity of Henna leaf and seed extracts was assessed against eight MDR isolates obtained from surface water, sewage, and abattoir leachate. The results showed exceptional antimicrobial activity, especially with seed methanol extracts demonstrating 100% inhibition against MDR bacteria, followed by the seed aqueous and leaf methanol (93.7%). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values confirmed the efficacy of Henna extracts. All the extracts displayed profound activity (AI) against S. enterica AGS2 strain. Citrobacter freundii ADAI was the most susceptible isolate. Our findings suggest that Henna extracts could serve as a viable, eco-friendly, and cost-effective solution for reducing MDR bacteria in environmental matrices, particularly in LMICs. This study highlights the potential of plant-based products in combating AMR in environmental matrices and promoting global public health. The results have significant implications for the development of sustainable sewage, abattoir leachate and water treatment strategies and the control of AMR in LMICs through plant-based solutions.
Yunpi Rougan Prescription in Treating Constipation-Predominant IBS: Clinical Observation and Gut Microbiota Effects
Authors: Wang Yongshuang , Xu Xiangxiang , Yang Shuhao , Gu Chao , An Zhentao , Ding Xuefei , Tian Yaozhou , Pan Chengyu , Li Hui 
Published: October 2024

Background:
Constipation-predominant irritable bowel syndrome (IBS-C) is a chronic
functional intestinal disease that can significantly reduce patients' quality of life.

Objective:
This study aims to evaluate the clinical effect and mechanism of YunPi RouGan
(YPRG) prescription on IBS-C patients with liver-depression and spleen-deficiency syndrome.

Methods:
42 IBS-C patients receiving treatment at Jiangsu Provincial Hospital of Integrated
Traditional Chinese and Western Medicine from May 2022 to March 2023 were recruited and
randomly assigned to either the treatment or control group, with 21 patients in each group. The
patients received either a YPRG prescription or a linalotide capsule for 4 weeks. A series of
scales were utilized to evaluate the clinical symptoms, psychological aspects, and quality of life
in IBS patients. Meanwhile, fresh fecal samples were collected to analyze the changes in gut microbiota
by 16SrDNA sequencing.

Results:
In terms of clinical treatment, both YPRG prescription and the first-line drug linaclotide
have similar effects for IBS-C. However, YPRG prescription has demonstrated significant improvements
in several symptoms, such as abdominal distension and belching. Furthermore, it has
been shown to upregulate the diversity of gut microbiota and induce changes in the types of
dominant microbiota in IBS-C patients. At the phylum level, Firmicutes and Bacteroides increased,
while Proteobacteria, actinobacteria, and desulphurobacteria decreased. At the genus
level, Bacteroides, Spirillum, Clostridium praxis, Roxella, Para-salmonella, Haemophilus, koala
bacillus, Micrococcus rare, Spirillum, and Streptococcus increased significantly.

result:
In terms of clinical treatment, both YPRG prescription and the first-line drug linaclotide have similar effects for IBS-C. However, YPRG prescription has demonstrated significant improvements in several symptoms, such as abdominal distension and belching. Furthermore, it has been shown to upregulate the diversity of gut microbiota and induce changes in the types of dominant microbiota in IBS-C patients. At the phylum level, Firmicutes and Bacteroides increased, while Proteobacteria, actinobacteria and desulphurobacteria decreased. At the genus level, Bacteroides, Spirillum, Clostridium praxis, Roxella, Para-salmonella, Haemophilus, koala bacillus, Micrococcus rare, Spirillum and Streptococcus increased significantly.

Conclusion:
The effect of YPRG prescription on improving the clinical symptoms of IBS-C
may be attributed to its potential to regulate gut microbiota.
KLF15 Transcription Activated Tachykinin Precursor 1 to Induce Perineural Invasion in Head and Neck Squamous Cell Carcinoma
Authors: Shen Yupeng , Shi Yong , Liu Xin , Zhang Ming , Zhou Liang , Xu Chengzhi 
Published: October 2024

Objective:
In this study, we aimed to identify novel biomarkers related to Peripheral
Neural Invasion (PNI) in head and neck squamous cell carcinoma (HNSCC).

Methods:
The PNI-related differentially expressed mRNAs (DE-mRNAs) in HNSCC were identified
to construct a PNI-related risk score model. The expression level and ROC curve for
Tachykinin Precursor 1 (TAC1) were calculated. Additionally, two kinds of in vitro models of
PNI were established for investigation, including the Matrigel-PNI model and the Transwell-PNI
model. Furthermore, the transcription factor of the TAC1 was predicted and verified by qRTPCR.

Results:
A total of 139 DE-mRNAs were identified in PNI positive and negative groups of
HNSCC patients. The risk-score marker model incorporating 20 PNI-related DE-mRNAs was
established. The TAC1 was identified as a potential highly expressed PNI marker, which exhibited
good performance in predicting PNI events. Patients with higher TAC1 expressions demonstrated
significantly shorter survival rates compared to those with lower TAC1 expressions in
HNSCC. Besides, the knockdown of TAC1 significantly repressed neural invasion in HNSCC
cells in vitro, according to the Matrigel-PNI model and Transwell-PNI model. Furthermore,
KLF15 was predicted and verified as a transcription activator of TAC1 in HNSCC.

Conclusion:
This study highlights that the activation of KLF15 transcription of TAC1 promotes
PNI in HNSCC cells, which provides guidance regarding the molecular diagnosis of PNI in
HNSCC cells.

conclusion:
This study highlights TAC1 as a PNI biomarker in HNSCC, and shed light on guiding molecular diagnosis of PNI in HNSCC.
Design and Development of [1,2,4]Triazolo[4,3-b][1,2,4]triazines as Potential Anticancer Agents with Genotoxicity and Apoptotic Activity
Authors: Khatir Zahra Zakeri , Valipour Mehdi , Rahmani Kasra , Aghajanshakeri Shaghayegh , Shokrzadeh Mohammad , Irannejad Hamid , Ghasemian Majid 
Published: October 2024

Aims:
In this current study, a new series of triazolo-triazine derivatives were designed
and synthesized as potential anticancer agents. Methods: The antiproliferative activity of the new
compounds was evaluated against three different cancerous cell lines (MDA-MB-231, HCT-116,
A549, and HT-29) using an MTT assay. To evaluate the mechanism of action, the ability of the best
compound in apoptosis induction and DNA damage was evaluated using the flow cytometry technique
and comet assays. Furthermore, molecular docking simulation was used to investigate their
interactions with the two targets, VEGFR2 and c-Met kinases. Results: Results showed that 6-(4-
bromophenyl)-3-((4-methoxybenzyl)thio)-[1,2,4]triazolo[4,3-b][1,2,4]triazine (8c) demonstrated
the best anti-proliferative activity against the human colorectal carcinoma cells HCT-116 with an
IC50 value of 38.7 ± 1.7 μM. In silico evaluations showed that the triazolo-triazine scaffold, along
with the methoxy substitution of compound 8c, was involved in creating effective H-bond interactions
in the active site of both targets. Conclusion: Our results showed that compound 8c significantly
increased cell death through apoptosis induction and caused a significant increase in genotoxicity.
Furthermore, it was found that the tested compound 8c, with a selectivity index of 1.74,
possessed selective antiproliferative activity towards the colorectal cancer cell line HCT-116 compared
to the normal fibroblast cell line. These findings could be useful in the development of novel
VEGFR2/c-Met dual-targeted inhibitors in the future.
Heterocyclic Derivatives in Parkinson’s Therapy: Recent Advances and Prospects
Authors: Kanupriya  , Sharma Vikram , Verma Suraj Pal , Mishra Isha , Mittal Ravi K , Biswas Tanya , Mishra Raghav 
Published: October 2024

Objectives:
This study aims to conduct a thorough examination of the most recent derivatives
that have been studied for their potential in treating Parkinson’s disease and to establish the
connection between the chemical structure of these derivatives and their effectiveness as therapeutic
agents.

Methods:
A thorough examination of the available literature was carried out to gather data about
the identification of pertinent research on derivatives aimed at addressing Parkinson’s disease. The
literature was analyzed to determine derivative chemical structures, modes of action, and preclinical
and clinical results.

Results:
To discover novel derivatives with therapeutic potential for Parkinson’s disease, our investigation
uncovered a variety of such chemical compounds. These derivatives cover a wide range of
chemical classes and demonstrate different ways of working, such as impacting neurotransmitter
systems, acting as antioxidants, and providing neuroprotective effects. In addition, an examination
of the SAR (Structure-activity relationship) uncovered important structural characteristics linked to
improved therapeutic effectiveness.

Conclusion:
This manuscript offers a thorough examination of the most recent derivatives aimed at
addressing Parkinson’s disease and their potential for therapeutic use. The understanding of SAR
and pharmacological attributes provides insights into the development and refining of novel medications
for Parkinson's disease treatment, promoting advancement in the field of neurotherapeutics.
Therapeutic Potential of 5α-Reductase Inhibitor 17-oxo-17a-aza-D-homo-5-androsten-3β-yl phenoxyacetate (17a-aza Steroid) in the Management of Prostate Cancer
Authors: Jan Gowsia , Bhat Swati , Dhawan Devinder Kumar , Dhingra Neelima , Chadha Vijayta Dani 
Published: October 2024

Background:
The present study highlights the comparative therapeutic efficacy of the
newly synthesized drug 17-Oximino-5-androsten-3β-yl 4-aminobenzoate (17a-aza steroid) and finasteride
for the treatment of experimentally induced prostate carcinogenesis in rats. Considering the
inhibitory activity of the oximes to 5α-reductase and the role of the ester group in augmenting the anti-
androgenic property, we decided to synthesize a compound with a lactam moiety in ring D and esters
at 3β position of androsterone nucleus as a more potent 5α-reductase inhibitor.

Methods:
In this study, 30 rats were divided into six groups, viz. Group I as normal controls, Group
II was treated with carcinogen N-methyl-N-nitrosourea (MNU) and hormone testosterone propionate
(TP) for inducing prostate cancer, Group III rats were treated with finasteride (10mg/Kg of body
weight for 8 weeks through gavage), Group IV was treated with 17a-aza steroid (10mg/Kg of body
weight for 8 weeks through gavage), whereas, Group V and Group VI were given finasteride and
17a-aza steroid, respectively, with similar doses as in Group III and IV after pretreatment with a carcinogen
(MNU) and hormone (TP).

Results:
The induction of carcinogenesis by treating animals with MNU+TP revealed a significant
reduction in weight loss as compared to the normal control group, which, however, increased following
17a-aza steroid treatment. Furthermore, the study witnessed a significant improvement in prostatic
biomarker, viz prostatic acid phosphatase (PAcP) in serum following finasteride and 17a-aza steroid
treatment to MNU+TP treated rats. Histological investigation of prostate tissue sections of 17aaza
steroid-treated prostate cancer rats showed milder hyperplastic glandular epithelium and exhibited
signs of recovery as compared to carcinogen-treated rats.

Conclusion:
The present findings are preliminary and suggest that the 17a-aza steroid has therapeutic
efficacy for the management of experimentally induced prostate cancer, as evidenced by changes in
prostate weight, PAcP levels, and histology. In comparison to finasteride, further exploration is needed
to assertively conclude its comparative therapeutic efficacy.
Discovery of Novel PTP1B Inhibitors by High-throughput Virtual Screening
Authors: Debnath Abhijit , Rani Anjna , Mazumder Rupa , Mazumder Avijit , Singh Rajesh Kumar , Sharma Shalini , Srivastava Shikha , Chaudhary Hema , Mishra Rashmi , Khurana Navneet , Sanchitra Jahanvi , Jan Sk Ashif 
Published: October 2024

Aim:
To Discover novel PTP1B inhibitors by high-throughput virtual screening

Background:
Type 2 Diabetes is a significant global health concern. According to projections,
the estimated number of individuals affected by the condition will reach 578 million by the year
2030 and is expected to further increase to 700 million deaths by 2045. Protein Tyrosine Phosphatase
1B is an enzymatic protein that has a negative regulatory effect on the pathways involved
in insulin signaling. This regulatory action ultimately results in the development of insulin
resistance and the subsequent elevation of glucose levels in the bloodstream. The proper
functioning of insulin signaling is essential for maintaining glucose homeostasis, whereas the
disruption of insulin signaling can result in the development of type 2 diabetes. Consequently,
we sought to utilize PTP1B as a drug target in this investigation.

Objective:
The purpose of our study was to identify novel PTP1B inhibitors as a potential
treatment for managing type 2 diabetes.

Methods:
To discover potent PTP1B inhibitors, we have screened the Maybridge HitDiscover
database by SBVS. Top hits have been passed based on various drug-likeness rules, toxicity
predictions, ADME assessment, Consensus Molecular docking, DFT, and 300 ns MD Simulations.

Results:
Two compounds have been identified with strong binding affinity at the active site of
PTP1B along with drug-like properties, efficient ADME, low toxicity, and high stability.

Conclusion:
The identified molecules could potentially manage T2DM effectively by inhibiting
PTP1B, providing a promising avenue for therapeutic strategies.
The Neuroprotective Action of Resveratrol Against Cognitive Impairments Induced by Lorazepam in Male Rats
Authors: Chakraborty Anup Kumar , Tiwari Prashant , Khobragade Deepak Shamrao , Kadiri Sunil Kumar , Sheikh Imran A , Thakur Jyoti 
Published: October 2024

Introduction/Aim:
The study examines how chronic resveratrol administration affects
behavioral and neurochemical changes caused by Lorazepam (LZP), a classical anti-anxiety
medicine associated with neurodegenerative and neurological problems.

Method:
Forty male rats were placed into four groups: a control group receiving 1% Tween 80,
the LZP group receiving 2 mg/kg/day, the Resveratrol group receiving 50 mg/kg/day, and the
LZP plus resveratrol group receiving the same doses of LZP and Resveratrol. Oral therapy was
given daily for 6 weeks. The animals were euthanized after open field and Y maze behavioral
tests. In specific brain regions, neurochemical analyses were performed on GABA, glutamic acid,
monoamines (norepinephrine, dopamine, and serotonin) and their metabolites, DNA fragmentation
(8-hydroxy-2–deoxyguanosine or 8-HdG), brain-derived neurotrophic factor (BDNF),
and Ca-ATPase.

Results:
Resveratrol therapy improved GABA, glutamic acid, monoamines, and their metabolites
in the cerebral cortex, hippocampus, and striatum. Additionally, it reduced DNA fragmentation (8-
HdG) and counteracted LZP-induced Ca-ATPase downregulation at a significant level (p < 0.05).
Resveratrol also reversed LZP-induced behavioral changes in the Y maze and open field tests.

Conclusion:
Resveratrol has anxiolytic-like actions like benzodiazepines and neuroprotective
capabilities against LZP-induced adverse effects.
NEXT-GEN Medicine: Designing Drugs to Fit Patient Profiles
Authors: kamal Raj , . Diksha , Paul Priyanka , Awasthi Ankit , Singh Amandeep 
Published: October 2024

Abstract:
Personalized medicine, with its focus on tailoring drug formulations to individual patient
profiles, has made significant strides in healthcare. The integration of genomics, biomarkers, nanotechnology,
3D printing, and real-time monitoring provides a comprehensive approach to optimizing
drug therapies on an individual basis. This review aims to highlight the recent advancements in personalized
medicine and its applications in various diseases, such as cancer, cardiovascular diseases,
diabetes mellitus, and neurodegenerative diseases. The review explores the integration of multiple
technologies in the field of personalized medicine, including genomics, biomarkers, nanotechnology,
3D printing, and real-time monitoring. As these technologies continue to evolve, we are entering
an era of truly personalized medicine that promises improved treatment outcomes, reduced adverse
effects, and a more patient-centric approach to healthcare. The advancements in personalized medicine
hold great promise for improving patient outcomes and reducing adverse effects, heralding a
new era in patient-centric healthcare.
Nattokinase Attenuated Excitatory Amino Acids and Cytokines Release and Restored Cerebral Blood Flow in a Thrombolytic Focal Cerebral Ischemic
Rat Model
Authors: Ramanathan M , KM Mohammed Jamshir , Jose Jerin M , Ganesan Nidhya 
Published: October 2024

Objective:
Nattokinase (NK), a protease enzyme present in traditional fermented Japanese
food, has shown fibrinolytic properties in vitro as well as in cardiac ischemia. In the present
study, the Neuroprotective effect of standardized NK was evaluated in the thrombolytic focal cerebral
ischemic model.

Methodology:
The parameters of behavioural assessment, cerebral blood flow, inflammatory mediators,
excitatory amino acids, and immunohistochemistry were measured to support the NK effect.
NK was administered at 150 and 300 mg/kg, and its effects were compared with streptokinase
(STK) (100μl/rat). Each mg of NK contains 5.5 Units of the enzyme, which can cause lysis of the
fibrin.

Results:
The results indicate that 7 days of treatment of 300 mg NK restored the cerebral blood
flow and prevented the release of cytokine and excitatory amino acids. Similarly, neurological
scores were reduced, and grip strength increased significantly with NK treatment. The GFAP and
synaptophysin staining of the hippocampus (CA1) and cerebrum have shown recovery of neurons
from ischemic damage in comparison to vehicle-treated ischemic-reperfused rats. The NK (300
mg/kg) fibrinolytic effect is comparable to STK treatment.

Conclusion:
To conclude, NK, a serine protease, protects the brain from ischemic degeneration in
thrombolytic cerebral ischemia. Consumption of this Japanese food might exhibit prophylactic activity.
Direct Current Ripple Component Detection Algorithm Based on Improved Variational Mode Decomposition
Authors: Meng Jing , Bai Jingfen , Cen Wei , Liu Lina , Chen Tengfei , Xu Suan , Zhang Zhijie 
Published: October 2024

Background:
The development of smart grids requires energy meters that enable accurate
measurements.

Objective:
In response to the current issue where Direct Current (DC) energy meters only measure
DC components and not ripple components, we propose to design a novel algorithm to achieve accurate
separation of DC components and ripple components in DC signals.

Methodology:
This patent proposes a precise detection algorithm for ripple components. The algorithm
is based on the Wavelet Decomposition combined with Flamingo Search Algorithm-Variational
Mode Decomposition (WD-FSA-VMD). Firstly, we analyze the ripple characteristics in the DC signal
and use the Wavelet Decomposition (WD) algorithm to separate the ripple components in the DC
signal accurately. Secondly, we utilize the Flamingo Search Algorithm (FSA) to optimize the number
of modal decompositions and the penalty factor in the Variational Mode Decomposition (VMD). This
allows us to accurately extract the ripple components. Finally, we conducted simulation experiments
comparing the improved algorithm with the original algorithm.

Results:
The experimental results indicate that the signal correlation coefficient obtained by the WDFSA-
VMD algorithm increases by 82.64% compared to traditional VMD, and it increases by 16.72%
compared to the combined Wavelet Decomposition with Whale Optimization Algorithm-Variational
Mode De-composition (WD-WOA-VMD).

Conclusion:
The improved algorithm we proposed has good adaptability and separation performance.
It is prospective for the new algorithm to provide a valuable reference to ripple detection
technology.