bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does adherence to the Mediterranean dietary pattern reduce asthma symptoms in children? A systematic review of observational studies
Authors: Papamichael Maria M , Itsiopoulos Catherine , Susanto Nugroho H , Erbas Bircan 
Published: August 2017

AbstractObjectiveThe purpose of the present systematic review was to synthesize evidence from the literature to assess efficacy of the Mediterranean dietary pattern in childhood asthma.Design/SettingA systematic search of six databases, three clinical trial registries and hand-search of peer-reviewed articles was conducted up to 29 October 2016. Inclusion criteria included exposure to a Mediterranean dietary pattern, measurement of asthma symptoms and study population of children aged <18 years. Quality assessment was conducted. Due to significant heterogeneity, meta-analysis was not feasible.ResultsOf the 436 articles identified, after removal of duplicates and based on inclusion criteria, fifteen observational studies conducted in Mediterranean and non-Mediterranean countries were relevant. No randomized controlled trials were retrieved. Twelve studies reported an inverse association between adherence to a Mediterranean dietary pattern and asthma in children, two studies showed no association and one study showed an increase in asthma symptoms. In fourteen out of fifteen studies, quality assessment checks revealed good reliability and validity among study methodologies.ConclusionsThe current systematic review revealed a consistent inverse relationship (protective) between a Mediterranean dietary pattern and asthma in children. Future well-designed randomized controlled trials are needed to provide solid evidence. Nevertheless, the existing level of evidence adds to the public health message relating to the beneficial effects of a Mediterranean-type diet in children suffering with asthma.
Table Talk: development of an observational tool to assess verbal feeding communications in early care and education settings
Authors: Swindle Taren , Rutledge Julie M , Dix Belynda , Whiteside-Mansell Leanne 
Published: August 2017

AbstractObjectiveChildren’s dietary intake impacts weight status and a range of short- and long-term health outcomes. Accurate measurement of factors that influence children’s diet is critical to the development and evaluation of interventions designed to improve children’s diets. The purpose of the current paper is to present the development of the Table Talk observational tool to measure early care and education teachers’ (ECET) verbal feeding communications.DesignAn observational tool to assess ECET verbal communication at mealtimes was deigned based on the extant literature. Trained observers conducted observations using the tool during lunch for both lead and assistant ECET. Descriptive statistics, test–retest for a subgroup, interclass correlations for each item, and comparisons between leads and assistants were conducted.SettingHead Start centres, Southern USA.SubjectsSeventy-five Head Start educators.ResultsOn average, 17·2 total verbal feeding communications (sd8·9) were observed per ECET. For lead ECET, the most prevalent Supportive Comment was Exploring Foods whereas for assistants Making Positive Comments was the most prevalent. Overall, lead ECET enacted more Supportive Comments than assistant ECET (F(2,72)=4·8,P=0·03). The most common Unsupportive Comment was Pressuring to Eat, with a mean of 3·8 (sd4·3) and a maximum of 25. There was no difference in Unsupportive Comments between lead and assistant ECET.ConclusionsTable Talk may be a useful tool to assess verbal feeding communications of ECET, with potential applications such as informing ECET training and assessing intervention efforts.
Recruiting young people with a visible difference to the YP Face IT feasibility trial: a qualitative exploration of primary care staff experiences
Authors: Hamlet Claire , Williamson Heidi , Harcourt Diana 
Published: August 2017

Background
Qualitative research methods embedded within feasibility trials are of significant value as they can provide important information for a definitive trial, often unable to be fulfilled by quantitative methods alone. In addition, such information can aid researchers running other trials or evaluating interventions on a similar topic.

Aim
This study aimed to explore GP and nurses’ experiences of recruiting to a trial exploring the feasibility of evaluating YP Face IT, a novel online psychosocial intervention to support young people with appearance-altering conditions.

Methods
During the recruitment period, a focus group with participating GPs and nurses explored recruitment challenges. In addition, at the end of the recruitment period, telephone interviews were conducted with eight GPs and nurses involved in recruiting to the study, in order to inform a definitive trial of YP Face IT. Transcripts were subjected to thematic analysis.

Findings
Despite reporting that the study was valuable and interesting, interviewees struggled to recruit in-consultation. They appeared to lack confidence in raising the sensitive issue of a visible difference and adopted strategies to avoid mentioning the topic. Participants felt the nature of the target population, as well as pressures of the primary care environment presented challenges to recruitment, but welcomed YP Face IT as an intervention that could address unmet support needs. Primary care staff may benefit from training to help them raise the subject of a visible difference with young people in order to identify those that require additional support.
Perspectives of patients, close relatives, nurses, and physicians on end-of-life medication management
Authors: Dees Marianne K. , Geijteman Eric C.T. , Dekkers Wim J.M. , Huisman Bregje A.A. , Perez Roberto S.G.M. , van Zuylen Lia , van der Heide Agnes , van Leeuwen Evert 
Published: August 2017

ABSTRACTObjective:Our aim was to gain insight into the perspectives of patients, close relatives, nurses, and physicians on medication management for patients with a life expectancy of less than 3 months.Method:We conducted an empirical multicenter study with a qualitative approach, including in-depth interviews with patients, relatives, nurses, specialists, and general practitioners (GPs). We used the constant comparative method and ATLAS.ti (v. 7.1) software for our analysis.Results:Saturation occurred after 18 patient cases (76 interviews). Some 5 themes covering 18 categories were identified: (1) priorities in end-of-life care, such as symptom management and maintaining hope; (2) appropriate medication use, with attention to unnecessary medication and deprescription barriers; (3) roles in decision making, including physicians in the lead, relatives' advocacy, and pharmacists as suppliers; (4) organization and communication (e.g., transparency of tasks and end-of-life conversations); and (5) prerequisites about professional competence, accessibility and quality of medical records, and financial awareness. Patients, relatives, nurses, specialists, and GPs varied in their opinions about these themes.Significance of Results:This study adds to our in-depth understanding of the complex practice of end-of-life medication management. It provides knowledge about the diversity of the perspectives of patients, close relatives, nurses, and physicians regarding beliefs, attitudes, knowledge, skills, behavior, work setting, the health system, and cultural factors related to the matter. Our results might help to draw an interdisciplinary end-of-life medication management guide aimed at stimulating a multidisciplinary and patient-centered pharmacotherapeutic care approach.
Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring
Authors: Curto Vincenzo F. , Marchiori Bastien , Hama Adel , Pappa Anna-Maria , Ferro Magali P. , Braendlein Marcel , Rivnay Jonathan , Fiocchi Michel , Malliaras George G. , Ramuz Marc , Owens Róisín M. 
Published: August 2017

AbstractFuture drug discovery and toxicology testing could benefit significantly from more predictive and multi-parametric readouts from in vitro models. Despite the recent advances in the field of microfluidics, and more recently organ-on-a-chip technology, there is still a high demand for real-time monitoring systems that can be readily embedded with microfluidics. In addition, multi-parametric monitoring is essential to improve the predictive quality of the data used to inform clinical studies that follow. Here we present a microfluidic platform integrated with in-line electronic sensors based on the organic electrochemical transistor. Our goals are two-fold, first to generate a platform to host cells in a more physiologically relevant environment (using physiologically relevant fluid shear stress (FSS)) and second to show efficient integration of multiple different methods for assessing cell morphology, differentiation, and integrity. These include optical imaging, impedance monitoring, metabolite sensing, and a wound-healing assay. We illustrate the versatility of this multi-parametric monitoring in giving us increased confidence to validate the improved differentiation of cells toward a physiological profile under FSS, thus yielding more accurate data when used to assess the effect of drugs or toxins. Overall, this platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices.
A three enzyme system to generate the Strychnos alkaloid scaffold from a central biosynthetic intermediate
Authors: Tatsis Evangelos C. , Carqueijeiro Inês , Dugé de Bernonville Thomas , Franke Jakob , Dang Thu-Thuy T. , Oudin Audrey , Lanoue Arnaud , Lafontaine Florent , Stavrinides Anna K. , Clastre Marc , Courdavault Vincent , O’Connor Sarah E. 
Published: August 2017

AbstractMonoterpene indole alkaloids comprise a diverse family of over 2000 plant-produced natural products. This pathway provides an outstanding example of how nature creates chemical diversity from a single precursor, in this case from the intermediate strictosidine. The enzymes that elicit these seemingly disparate products from strictosidine have hitherto been elusive. Here we show that the concerted action of two enzymes commonly involved in natural product metabolism—an alcohol dehydrogenase and a cytochrome P450—produces unexpected rearrangements in strictosidine when assayed simultaneously. The tetrahydro-β-carboline of strictosidine aglycone is converted into akuammicine, a Strychnos alkaloid, an elusive biosynthetic transformation that has been investigated for decades. Importantly, akuammicine arises from deformylation of preakuammicine, which is the central biosynthetic precursor for the anti-cancer agents vinblastine and vincristine, as well as other biologically active compounds. This discovery of how these enzymes can function in combination opens a gateway into a rich family of natural products.
Lapsyn controls branch extension and positioning of astrocyte-like glia in the Drosophila optic lobe
Authors: Richier Benjamin , Vijandi Cristina de Miguel , Mackensen Stefanie , Salecker Iris 
Published: August 2017

AbstractAstrocytes have diverse, remarkably complex shapes in different brain regions. Their branches closely associate with neurons. Despite the importance of this heterogeneous glial cell type for brain development and function, the molecular cues controlling astrocyte branch morphogenesis and positioning during neural circuit assembly remain largely unknown. We found that in the Drosophila visual system, astrocyte-like medulla neuropil glia (mng) variants acquire stereotypic morphologies with columnar and layered branching patterns in a stepwise fashion from mid-metamorphosis onwards. Using knockdown and loss-of-function analyses, we uncovered a previously unrecognized role for the transmembrane leucine-rich repeat protein Lapsyn in regulating mng development. lapsyn is expressed in mng and cell-autonomously required for branch extension into the synaptic neuropil and anchoring of cell bodies at the neuropil border. Lapsyn works in concert with the fibroblast growth factor (FGF) pathway to promote branch morphogenesis, while correct positioning is essential for mng survival mediated by gliotrophic FGF signaling.
Genetic cargo and bacterial species set the rate of vesicle-mediated horizontal gene transfer
Authors: Tran Frances , Boedicker James Q. 
Published: August 2017

AbstractMost bacteria release extracellular vesicles (EVs). Recent studies have found these vesicles are capable of gene delivery, however the consequences of vesicle-mediated transfer on the patterns and rates of gene flow within microbial communities remains unclear. Previous studies have not determined the impact of both the genetic cargo and the donor and recipient species on the rate of vesicle-mediated gene exchange. This report examines the potential for EVs as a mechanism of gene transfer within heterogeneous microbial populations. EVs were harvested from three species of Gram-negative microbes carrying different plasmids. The dynamics of gene transfer into recipient species was measured. This study demonstrates that vesicles enable gene exchange between five species of Gram-negative bacteria, and that the identity of the genetic cargo, donor strain, and recipient strain all influence gene transfer rates. Each species released and acquired vesicles containing genetic material to a variable degree, and the transfer rate did not correlate with the relatedness of the donor and recipient species. The results suggest that EVs may be a general mechanism to exchange non-specialized genetic cargo between bacterial species.
Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria
Authors: Kaduskar Rahul D. , Scala Giulia Della , Al Jabri Zaaima J. H. , Arioli Stefania , Musso Loana , Oggioni Marco R. , Dallavalle Sabrina , Mora Diego 
Published: August 2017

AbstractPromysalin was previously described as a narrow spectrum molecule with a unique species-specific activity against Pseudomonas aeruginosa. Here we demonstrate that promysalin is active against Gram-positive and Gram-negative bacteria using a microdilution assay. Promysalin acts on Gram-positive bacteria with a mechanism of action involving cell membrane damage with leakage of intracellular components. The evaluation of MICs and MBCs on 11 promysalin analogs, synthesized utilizing diverted total synthesis, allowed the identification of the structural moieties potentially involved in cell membrane interaction and damage. The mechanism of action of promysalin against Gram-negative bacteria is still not clarified, even if a synergistic effect with the bisguanidine chlorhexidine on cell membrane disruption has been observed.
Inconsistent estimates of forest cover change in China between 2000 and 2013 from multiple datasets: differences in parameters, spatial resolution, and definitions
Authors: Li Yan , Sulla-Menashe Damien , Motesharrei Safa , Song Xiao-Peng , Kalnay Eugenia , Ying Qing , Li Shuangcheng , Ma Zongwen 
Published: August 2017

AbstractThe Chinese National Forest Inventory (NFI) has reported increased forest coverage in China since 2000, however, the new satellite-based dataset Global Forest Change (GFC) finds decreased forest coverage. In this study, four satellite datasets are used to investigate this discrepancy in forest cover change estimates in China between 2000 and 2013: forest cover change estimated from MODIS Normalized Burn Ratio (NBR), existing MODIS Land Cover (LC) and Vegetation Continuous Fields (VCF) products, and the Landsat-based GFC. Among these satellite datasets, forest loss shows much better agreement in terms of total change area and spatial pattern than do forest gain. The net changes in forest cover as a proportion of China’s land area varied widely from increases of 1.56% in NBR, 1.93% in VCF, and 3.40% in LC to a decline of −0.40% in GFC. The magnitude of net forest increase derived from MODIS datasets (1.56–3.40%) is lower than that reported in NFI (3.41%). Algorithm parameters, different spatial resolutions, and inconsistent forest definitions could be important sources of the discrepancies. Although several MODIS datasets support an overall forest increase in China, the direction and magnitude of net forest change is still unknown due to the large uncertainties in satellite-derived estimates.
Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
Authors: Lei Wei-Qiang , Lok James B. , Yuan Wang , Zhang Yue-Zhou , Stoltzfus Jonathan D. , Gasser Robin B. , He Si-Yuan , Zhou Huan , Zhou Rui , Zhao Jun-Long , Hu Min 
Published: August 2017

AbstractRIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in yeast and humans. However, little is known about their functions in parasitic nematodes. In the present study, we have isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein kinase (Ss-RIOK-2) encoding gene (Ss-riok-2) from Strongyloides stercoralis, a medically important parasitic nematode (Order Rhabditida). A three-dimensional structure (3D) model of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase (Ct-RIOK-2) crystal structure 4GYG as a template. A docking study revealed some critical sites for ATP binding and metal binding. The putative promoter of Ss-riok-2 contains a number of conserved elements. RNAseq analysis revealed the highest levels of the Ss-riok-2 transcript in free-living females and parasitic females. To identify anatomical patterns of Ss-riok-2 expression in S. stercoralis, we observed expression patterns of a transgene construct encoding green fluorescent protein under the Ss-riok-2 promoter in post free-living S. stercoralis. Expression driven by this promoter predominated in intestinal cells. This study demonstrates significant advancement in molecular and cellular biological study of S. stercoralis and of parasitic nematodes generally, and provides a foundation for further functional genomic studies.
Transcriptome analysis reveals long intergenic non-coding RNAs involved in skeletal muscle growth and development in pig
Authors: Zou Cheng , Li Jingxuan , Luo Wenzhe , Li Long , Hu An , Fu Yuhua , Hou Ye , Li Changchun 
Published: August 2017

AbstractLong intergenic non-coding RNAs (lincRNAs) play essential roles in numerous biological processes and are widely studied. The skeletal muscle is an important tissue that plays an essential role in individual movement ability. However, lincRNAs in pig skeletal muscles are largely undiscovered and their biological functions remain elusive. In this study, we assembled transcriptomes using RNA-seq data published in previous studies of our laboratory group and identified 323 lincRNAs in porcine leg muscle. We found that these lincRNAs have shorter transcript length, fewer exons and lower expression level than protein-coding genes. Gene ontology and pathway analyses indicated that many potential target genes (PTGs) of lincRNAs were involved in skeletal-muscle-related processes, such as muscle contraction and muscle system process. Combined our previous studies, we found a potential regulatory mechanism in which the promoter methylation of lincRNAs can negatively regulate lincRNA expression and then positively regulate PTG expression, which can finally result in abnormal phenotypes of cloned piglets through a certain unknown pathway. This work detailed a number of lincRNAs and their target genes involved in skeletal muscle growth and development and can facilitate future studies on their roles in skeletal muscle growth and development.
TRPM6 and TRPM7 differentially contribute to the relief of heteromeric TRPM6/7 channels from inhibition by cytosolic Mg2+ and Mg·ATP
Authors: Ferioli Silvia , Zierler Susanna , Zaißerer Joanna , Schredelseker Johann , Gudermann Thomas , Chubanov Vladimir 
Published: August 2017

AbstractTRPM6 and its homologue TRPM7 are α-kinase-coupled divalent cation-selective channels activated upon reduction of cytosolic levels of Mg2+ and Mg·ATP. TRPM6 is vital for organismal Mg2+ balance. However, mechanistically the cellular role and functional nonredundancy of TRPM6 remain incompletely understood. Comparative analysis of native currents in primary cells from TRPM6- versus TRPM7-deficient mice supported the concept that native TRPM6 primarily functions as a constituent of heteromeric TRPM6/7 channels. However, heterologous expression of the human TRPM6 protein engendered controversial results with respect to channel characteristics including its regulation by Mg2+ and Mg·ATP. To resolve this issue, we cloned the mouse TRPM6 (mTRPM6) cDNA and compared its functional characteristics to mouse TRPM7 (mTRPM7) after heterologous expression. Notably, we observed that mTRPM6 and mTRPM7 differentially regulate properties of heteromeric mTRPM6/7 channels: In the presence of mTRPM7, the extreme sensitivity of functionally expressed homomeric mTRPM6 to Mg2+ is tuned to higher concentrations, whereas mTRPM6 relieves mTRPM7 from the tight inhibition by Mg·ATP. Consequently, the association of mTRPM6 with mTRPM7 allows for high constitutive activity of mTRPM6/7 in the presence of physiological levels of Mg2+ and Mg·ATP, thus laying the mechanistic foundation for constant vectorial Mg2+ transport specifically into epithelial cells.
Ecological speciation in a generalist consumer expands the trophic niche of a dominant predator
Authors: Thomas Stephen M. , Harrod Chris , Hayden Brian , Malinen Tommi , Kahilainen Kimmo K. 
Published: August 2017

AbstractEcological speciation – whereby an ancestral founder species diversifies to fill vacant niches – is a phenomenon characteristic of newly formed ecosystems. Despite such ubiquity, ecosystem-level effects of such divergence remain poorly understood. Here, we compared the trophic niche of European whitefish (Coregonus lavaretus) and their predators in a series of contrasting subarctic lakes where this species had either diversified into four ecomorphologically distinct morphs or instead formed monomorphic populations. We found that the trophic niche of whitefish was almost three times larger in the polymorphic than in the monomorphic lakes, due to an increase in intraspecific specialisation. This trophic niche expansion was mirrored in brown trout (Salmo trutta), a major predator of whitefish. This represents amongst the first evidence for ecological speciation directly altering the trophic niche of a predator. We suggest such mechanisms may be a common and important – though presently overlooked – factor regulating trophic interactions in diverse ecosystems globally.
Neuromodulatory circuit effects on Drosophila feeding behaviour and metabolism
Authors: Eriksson Anders , Raczkowska Marlena , Navawongse Rapeechai , Choudhury Deepak , Stewart James C. , Tang Yi Ling , Wang Zhiping , Claridge-Chang Adam 
Published: August 2017

AbstractAnimals have evolved to maintain homeostasis in a changing external environment by adapting their internal metabolism and feeding behaviour. Metabolism and behaviour are coordinated by neuromodulation; a number of the implicated neuromodulatory systems are homologous between mammals and the vinegar fly, an important neurogenetic model. We investigated whether silencing fly neuromodulatory networks would elicit coordinated changes in feeding, behavioural activity and metabolism. We employed transgenic lines that allowed us to inhibit broad cellular sets of the dopaminergic, serotonergic, octopaminergic, tyraminergic and neuropeptide F systems. The genetically-manipulated animals were assessed for changes in their overt behavioural responses and metabolism by monitoring eleven parameters: activity; climbing ability; individual feeding; group feeding; food discovery; both fed and starved respiration; fed and starved lipid content; and fed/starved body weight. The results from these 55 experiments indicate that individual neuromodulatory system effects on feeding behaviour, motor activity and metabolism are dissociated.