bluuebunny/crossref_metadata_2025_split huggingface dataset:
Native extracellular matrix preserves mesenchymal stem cell “stemness” and differentiation potential under serum-free culture conditions
Authors: Rakian Rubie , Block Travis J. , Johnson Shannan M. , Marinkovic Milos , Wu Junjie , Dai Qiuxia , Dean David D. , Chen Xiao-Dong 
Published: December 2015

Abstract
Introduction
Bone marrow-derived mesenchymal stem cells (BM-MSCs) for clinical use should not be grown in media containing fetal bovine serum (FBS), because of serum-related concerns over biosafety and batch-to-batch variability. Previously, we described the preparation and use of a cell-free native extracellular matrix (ECM) made by bone marrow cells (BM-ECM) which preserves stem cell properties and enhances proliferation. Here, we compare colony-forming ability and differentiation of MSCs cultured on BM-ECM with a commercially available matrix (CELLstart™) and tissue culture plastic (TCP) under serum-free conditions.

Methods
Primary MSCs from freshly isolated bone marrow-derived mononuclear cells or passaged MSCs (P1) were grown in serum-containing (SCM) or serum-free (SFM) media on BM-ECM, CELLstart™, or TCP substrates. Proliferation, cell composition (phenotype), colony-forming unit replication, and bone morphogenetic protein-2 (BMP-2) responsiveness were compared among cells maintained on the three substrates.

Results
Proliferation of primary BM-MSCs was significantly higher in SCM than SFM, irrespectively of culture substrate, suggesting that the expansion of these cells requires SCM. In contrast, passaged cells cultured on BM-ECM or CELLstart™ in SFM proliferated to nearly the same extent as cells in SCM. However, morphologically, those on BM-ECM were smaller and more aligned, slender, and long.
Cells grown for 7 days on BM-ECM in SFM were 20–40 % more positive for MSC surface markers than cells cultured on CELLstart™. Cells cultured on TCP contained the smallest number of cells positive for MSC markers. MSC colony-forming ability in SFM, as measured by CFU-fibroblasts, was increased 10-, 9-, and 2-fold when P1 cells were cultured on BM-ECM, CELLstart™, and TCP, respectively. Significantly, CFU-adipocyte and -osteoblast replication of cells grown on BM-ECM was dramatically increased over those on CELLstart™ (2X) and TCP (4-7X). BM-MSCs, cultured in SFM and treated with BMP-2, retained their differentiation capacity better on BM-ECM than on either of the other two substrates.

Conclusions
Our findings indicate that BM-ECM provides a unique microenvironment that supports the colony-forming ability of MSCs in SFM and preserves their stem cell properties. The establishment of a robust culture system, combining native tissue-specific ECM and SFM, provides an avenue for preparing significant numbers of potent MSCs for cell-based therapies in patients.
Existence of solutions of first-order differential equations via a fixed point theorem for discontinuous operators
Authors: Sestelo Rubén Figueroa , Pouso Rodrigo López 
Published: December 2015

AbstractWe use a recent Schauder-type result for discontinuous operators in order to look for solutions for first-order differential equations subject to initial functional conditions. We show how this abstract fixed-point result allows us to consider a nonlinearity which can be strongly discontinuous. Some examples of applications and comparison with recent literature are included.
Common coupled fixed point theorems for θ-ψ-contraction mappings endowed with a directed graph
Authors: Suantai Suthep , Charoensawan Phakdi , Lampert Tatjana Aleksic 
Published: December 2015

AbstractIn this paper, we present some existence and uniqueness results for coupled coincidence point and common fixed point of θ-ψ-contraction mappings in complete metric spaces endowed with a directed graph. Our results generalize the results obtained by Kadelburg et al. (Fixed Point Theory Appl. 2015:27, 2015, doi:10.1007/s11590-013-0708-4). We also have an application to some integral system to support the results.
Complete genome of Pseudomonas chlororaphis strain UFB2, a soil bacterium with antibacterial activity against bacterial canker pathogen of tomato
Authors: Deng Peng , Wang Xiaoqiang , Baird Sonya M. , Lu Shi-En 
Published: December 2015

AbstractStrain UFB2 was isolated from a soybean field soil in Mississippi and identified as a member of Pseudomonas chlororaphis. Strain UFB2 has a broad-spectrum antimicrobial activity against common soil-borne pathogens. Plate assays showed that strain UFB2 was especially efficient in inhibiting the growth of Clavibacter michiganensis 1–07, the causal agent of the devastating bacterial canker of tomato. Here, the complete genome sequence of P. chlororaphis strain UFB2 is reported and described. The strain UFB2 genome consists of a circular chromosome of 6,360,256 bp of which 87.86 % are protein-coding bases. Genome analysis revealed multiple gene islands encoding various secondary metabolites such as 2,4-diacetylphloroglucinol. Further genome analysis will provide more details about strain UFB2 antibacterial activities mechanisms and the use of this strain as a potential biocontrol agent.
Complete genome sequence of Salinicoccus halodurans H3B36, isolated from the Qaidam Basin in China
Authors: Jiang Kai , Xue Yanfen , Ma Yanhe 
Published: December 2015

AbstractSalinicoccus halodurans H3B36 is a moderately halophilic bacterium isolated from a sediment sample of Qaidam Basin at 3.2 m vertical depth. Strain H3B36 accumulate Nα-acetyl-α-lysine as compatible solute against salinity and heat stresses and may have potential applications in industrial biotechnology. In this study, we sequenced the genome of strain H3B36 using single molecule, real-time sequencing technology on a PacBio RS II instrument. The complete genome of strain H3B36 was 2,778,379 bp and contained 2,853 protein-coding genes, 12 rRNA genes, and 61 tRNA genes with 58 tandem repeats, six minisatellite DNA sequences, 11 genome islands, and no CRISPR repeat region. Further analysis of epigenetic modifications revealed the presence of 11,000 m4C-type modified bases, 7,545 m6A-type modified bases, and 89,064 other modified bases. The data on the genome of this strain may provide an insight into the metabolism of Nα-acetyl-α-lysine.
Mental state decoding impairment in major depression and
borderline personality disorder: Meta-analysis
Authors: Richman Mara J. , Unoka Zsolt 
Published: December 2015

BackgroundPatients with major depression and borderline personality disorder are
characterised by a distorted perception of other people's intentions.
Deficits in mental state decoding are thought to be the underlying cause
of this clinical feature.AimsTo examine, using meta-analysis, whether mental state decoding abilities
in patients with major depression and borderline personality disorder
differ from those of healthy controls.MethodA systematic review of 13 cross-sectional studies comparing Reading in
the Mind of the Eyes Test (RMET) accuracy performance of patients with
major depression or borderline personality disorder and healthy
age-matched controls (n = 976). Valence scores, where
reported, were also assessed.ResultsLarge significant deficits were seen for global RMET performance in
patients with major depression (d =–0.751). The positive
RMET valence scores of patients with depression were significantly worse;
patients with borderline personality disorder had worse neutral scores.
Both groups were worse than controls. Moderator analysis revealed that
individuals with comorbid borderline personality disorder and major
depression did better than those with borderline personality disorder
alone on accuracy. Those with comorbid borderline personality disorder
and any cluster B or C personality disorder did worse than borderline
personality disorder alone. Individuals with both borderline personality
disorder and major depression performed better then those with borderline
personality disorder without major depression for positive valence.ConclusionsThese findings highlight the relevance of RMET performance in patients
with borderline personality disorder and major depression, and the
importance of considering comorbidity in future analysis.
Case study of posts before and after a suicide on a Swedish
internet forum
Authors: Westerlund Michael , Hadlaczky Gergö , Wasserman Danuta 
Published: December 2015

SummaryWebsites and discussion forums have become an important and sometimes
controversial source of information on suicide. Using a case report, our aim
was to examine the responses, attitudes and beliefs that were communicated
on a forum before, during and after a suicide act. We undertook two related
analyses: a qualitative investigation of the messages that were posted
before the suicide and a combined qualitative–quantitative analysis of the
messages posted during and after the suicide. Nearly half the posted
messages before the suicide encouraged the victim to complete the suicidal
act, and a surprising number of posts after the suicide expressed
excitement, although around half of the posts considered the suicide to be
tragic. It is of great importance to increase awareness of suicide signals
and understanding about how to respond to individuals who communicate
suicide intentions on different forums on the internet.
ECT practices in Iraq: a national audit
Authors: Alhemiary Nesif , Ali Zainab , Abbas Mohammed J. 
Published: December 2015

Aims and methodThis national audit examined practice of electroconvulsive therapy (ECT) in Iraq against local standards. Data were collected by a questionnaire sent to heads of departments or medical directors in the 10 Iraqi hospitals which provide ECT and by examining case notes of all patients who had ECT in the first 6 months of 2013.ResultsOf the 26 psychiatric hospitals in Iraq, 10 provide ECT. There were some resource shortcomings in the ECT clinics (e.g. only 2 had a minimum of 2 rooms and all had no EEG monitoring). During the audit period, 251 patients had ECT. The mean age was 36.2 years and 51.8% were males. Bilateral ECT was used in all cases, general anaesthesia in 77.15%. The main indication for ECT was schizophrenia, followed by severe depression, resistant mania, catatonia and others.Clinical implicationsMore work is needed to ensure all patients receive modified ECT. ECT is still used widely for schizophrenia. This needs further exploration and training.
Newspaper reporting of homicide-suicide and mental illness
Authors: Flynn Sandra , Gask Linda , Shaw Jenny 
Published: December 2015

Aims and methodTo explore the portrayal of homicide-suicide in newspaper articles, particularly how mental illness was reported. We carried out a qualitative study in England and Wales (2006–2008). Data from newspaper articles obtained via the LexisNexis database were used to examine a consecutive series of 60 cases.ResultsA fascination with extreme violence, vulnerable victims and having someone to blame made homicide-suicides newsworthy. Some offenders were portrayed in a stereotypical manner and pejorative language was used to describe mental illness. The findings showed evidence of inaccurate and speculative reference to mental disorder in newspaper reports.Clinical implicationsThe media should avoid speculation on people's mental state. Accurate reporting is essential to reduce stigma of mental illness, which may in turn encourage people to seek help if they experience similar emotional distress.
Mobile telephone apps in mental health practice: uses, opportunities and challenges
Authors: Marley Justin , Farooq Saeed 
Published: December 2015

SummarySmartphones are used by patients and clinicians alike. Vast numbers of software applications (apps) run on smartphones and carry out useful functions. Clinician- and patient-oriented mental health apps have been developed. In this article, we provide an overview of apps that are relevant for mental health. We look at clinician-oriented apps that support assessment, diagnosis and treatment as well as patient-oriented apps that support education and self-management. We conclude by looking at the challenges that apps pose with a discussion of possible solutions.
YouTube and ‘psychiatry’
Authors: Gordon Robert , Miller John , Collins Noel 
Published: December 2015

SummaryYouTube is a video-sharing website that is increasingly used to share and disseminate health-related information, particularly among younger people. There are reports that social media sites, such as YouTube, are being used to communicate an anti-psychiatry message but this has never been confirmed in any published analysis of YouTube clip content. This descriptive study revealed that the representation of ‘psychiatry’ during summer 2012 was predominantly negative. A subsequent smaller re-analysis suggests that the negative portrayal of ‘psychiatry’ on YouTube is a stable phenomenon. The significance of this and how it could be addressed are discussed.
Application and simplified proof of a sharp L2 extension theorem
Authors: Ohsawa Takeo 
Published: December 2015

AbstractAs an application of a sharp L2 extension theorem for holomorphic functions in Guan and Zhou, a stability theorem for the boundary asymptotics of the Bergman kernel is proved. An alternate proof of the extension theorem is given, too. It is a simplified proof in the sense that it is free from ordinary differential equations.
Analysis of neural progenitors from embryogenesis to juvenile adult inXenopus laevisreveals biphasic neurogenesis and continuous lengthening of the cell cycle
Authors: Thuret Raphaël , Auger Hélène , Papalopulu Nancy 
Published: December 2015

ABSTRACTXenopus laevis is a prominent model system for studying neural development, but our understanding of the long-term temporal dynamics of neurogenesis remains incomplete. Here, we present the first continuous description of neurogenesis in X.laevis, covering the entire period of development from the specification of neural ectoderm during gastrulation to juvenile frog. We have used molecular markers to identify progenitors and neurons, short-term bromodeoxyuridine (BrdU) incorporation to map the generation of newborn neurons and dual pulse S-phase labelling to characterise changes in their cell cycle length. Our study revealed the persistence of Sox3-positive progenitor cells from the earliest stages of neural development through to the juvenile adult. Two periods of intense neuronal generation were observed, confirming the existence of primary and secondary waves of neurogenesis, punctuated by a period of quiescence before metamorphosis and culminating in another period of quiescence in the young adult. Analysis of multiple parameters indicates that neural progenitors alternate between global phases of differentiation and amplification and that, regardless of their behaviour, their cell cycle lengthens monotonically during development, at least at the population level.
Apical constriction and epithelial invagination are regulated by BMP activity
Authors: Jidigam Vijay K. , Srinivasan Raghuraman C. , Patthey Cedric , Gunhaga Lena 
Published: December 2015

ABSTRACTEpithelial invagination is a morphological process in which flat cell sheets transform into three-dimensional structures through bending of the tissue. It is accompanied by apical constriction, in which the apical cell surface is reduced in relation to the basal cell surface. Although much is known about the intra-cellular molecular machinery driving apical constriction and epithelial invagination, information of how extra-cellular signals affect these processes remains insufficient. In this study we have established several in vivo assays of placodal invagination to explore whether the external signal BMP regulates processes connected to epithelial invagination. By inhibiting BMP activity in prospective cranial placodes, we provide evidence that BMP signals are required for RhoA and F-actin rearrangements, apical constriction, cell elongation and epithelial invagination. The failure of placode invagination after BMP inhibition appears to be a direct consequence of disrupted apical accumulation of RhoA and F-actin, rather than changes in cell death or proliferation. In addition, our results show that epithelial invagination and acquisition of placode-specific identities are two distinct and separable developmental processes. In summary, our results provide evidence that BMP signals promote epithelial invagination by acting upstream of the intracellular molecular machinery that drives apical constriction and cell elongation.
Business reputation of organisation in the context of the notion “social responsibility”: moral and ethical dimensions of management
Authors: Шекелета Владислав , Shekeleta Vladislav , Дмитриева Ирина , Dmitrieva Irina , Кустова Наталья , Kustova Natalya 
Published: December 2015

The article is devoted to ethical aspects of reputation management. The authors, bringing together different
research methodology such as categorical and phenomenological analysis, examine the moral categories associated
with the concept of reputation. Therefore, the concept of “image”, “goodwill” reveals its importance
in the context of the ethical categories and moral manifestations of consciousness, such as authority, social
responsibility, honesty, integrity, respect for the interests of another. In the future human dimensions of management,
such as corporate ethics, corporate culture, as well as the position subjectivist approach to research
of the image of the organization is ethical-oriented concept of reputation management. The author defends the
position according to which it’s necessary to examine social entrepreneurship in all its dimensions, which is
designed to assess the activity of the enterprise by connecting scientists, as well as components of civil society,
from the perspective of human values – moral and ethical categories. In this understanding, social expertise
acts as “ethical expertise”. In conjunction with the notion and phenomenon of corporate ethics there are generalizations
in the context of General ethical categories. The authors show that corporate ethics is the basis, the
Foundation for formation of business reputation, corporate image and other ethical components of corporate
reputation. In connection with the concept of ethically-oriented management protected in the article there are
the prospects for use of this methodological approach for its application not only to build a successful business
reputation of the organization, but also in the process of creating a brand, developing a content marketing
strategy.
Innovation management companies in the fuzzy set theory
Authors: Мосалев Антон , Mosalev Anton 
Published: December 2015

The problem of management of uncertainties in making business decisions is a relatively new science and practice.
However, the question of development strategy innovation for companies in a non-obvious consequences of every decision
is important. It is known that there are uncertainties in the outer, inner and an adjacent environment, furthermore,
installed and timing of their occurrence, and is defined as a regional factor. The question remains open on the management
of innovation processes in the company, taking into account the impact of each specific type of uncertainty.
To solve the identified problem has been specifically developed an approach based on the theory of fuzzy sets. Thus
was created the algorithm processing expert survey of entrepreneurs (more than 6700 units), which are in its production
portfolio of innovative projects. In the framework of each of uncertainty determined by the following parameters:
at what time each uncertainty manifest itself; which will serve as a condition of occurrence of uncertainty
(internal, external, or conjugate organizational environment); is it possible to predict in advance whether or not
this uncertainty; that it will stimulate the appearance of one or another of uncertainty (the nature of occurrence).
For example, to predict the emergence of a situation of uncertainty can only be in the following generating their
nature: the market, the system of relations, technical and technological, legal, financial and timely. In the case of
organizational, informational, labor uncertainty is impossible to predict the nature.
The article also presents the ratio of occurrence of designated parameters uncertainties in the implementation of
innovations in time, the environment, and the possibility of predicting the nature of existence itself uncertainties.
Research study financed from Russian Foundation for Basic Research in the year 2014–2015 as research project
No. 14–06–00051 «The implementation of innovative projects and processes under conditions of uncertainty».
Essence of the concept «educational tourism» and its basic principles
Authors: Гусейнова Алена , Guseynova Alena 
Published: December 2015

The article is devoted to theoretical issues in the field of educational tourism. It examines the origins of the concept
of «educational tourism» and provides some options for definitions of key components – «education», «tourism».
The article describes the interpretation of the concept of «educational tourism» domestic and foreign authors.
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Basic principles of educational tourism. The degree of scientific and practical novelty is taken by the author who
tries to highlight the principles of educational tourism through informative data about the concept and types of
educational tourism in accordance with recognized classifications. The author identifies eight principles, six of
which are directly to consider the directions of tourist activity. Based on the definition of «educational tourism»
a systematic and pedagogical principle provides. According to the existing classifications of this type of tourism,
highlights the layering, are evidence of this assertion. Educational tourism involves learning specific disciplines,
so there is a possibility of extension of the principle of interdisciplinarity. Educational tourism has the gradation
of destination, in accordance with what is determined by the principle of interculturality. Another fundamental
principle of educational tourism is called the principle of carrying capacity. These principles of educational tourism
are illustrated by diagrams. Customer orientation and temporal constraints are considered applicable for all types
of tourism, including educational. The author attempts to bring the methodological principles of implementation
and development of educational tourism, according to the conceptual apparatus of the recognized classifications
and other legislative acts listed in the article documentary sources. This tourist activity is relevant, widely spread in
the world industry and is developing in Russia. A detailed study of the theoretical aspects, highlighted by the author
in this article is required to be examined in practical issues. The article can be useful as introductory material for
novice scientists, undergraduates, students and teachers in the preparation of notes to English classes.
Multiplicative effects of tourism on the economy of the host country
Authors: Караулова Наталия , Karaulova Nataliya 
Published: December 2015

This article presents the methods of calculating the tourism multiplier applied to the travel industry. They consider
the impact of tourist flows on the host economy in different directions. The article forms the scheme of redistribution
of expenses of foreign tourists, and on its basis the scheme of multiplicative expansion of incomes of residents
of a tourist destination. The formula multiplier of international tourism, based on the principle of cyclicality of the
economic system is used.