bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Self-Consistent Field Model of Polymer/Nanoparticle Mixture
Authors: Chen Kang , Li Hui-shu , Zhang Bo-kai , Li Jian , Tian Wen-de 
Published: February 2016

AbstractField-theoretical method is efficient in predicting assembling structures of polymeric systems. However, it’s challenging to generalize this method to study the polymer/nanoparticle mixture due to its multi-scale nature. Here, we develop a new field-based model which unifies the nanoparticle description with the polymer field within the self-consistent field theory. Instead of being “ensemble-averaged” continuous distribution, the particle density in the final morphology can represent individual particles located at preferred positions. The discreteness of particle density allows our model to properly address the polymer-particle interface and the excluded-volume interaction. We use this model to study the simplest system of nanoparticles immersed in the dense homopolymer solution. The flexibility of tuning the interfacial details allows our model to capture the rich phenomena such as bridging aggregation and depletion attraction. Insights are obtained on the enthalpic and/or entropic origin of the structural variation due to the competition between depletion and interfacial interaction. This approach is readily extendable to the study of more complex polymer-based nanocomposites or biology-related systems, such as dendrimer/drug encapsulation and membrane/particle assembly.
Phosphorylation of serine residue modulates cotton Di19-1 and Di19-2 activities for responding to high salinity stress and abscisic acid signaling
Authors: Qin Li-Xia , Nie Xiao-Ying , Hu Rong , Li Gang , Xu Wen-Liang , Li Xue-Bao 
Published: February 2016

AbstractDi19 (drought-induced protein 19) family is a novel type of Cys2/His2 zinc-finger proteins. In this study, we demonstrated that cotton Di19-1 and Di19-2 (GhDi19-1/-2) proteins could be phosphorylated in vitro by the calcium-dependent protein kinase (CDPK). Mutation of Ser to Ala in N-terminus of GhDi19-1/-2 led to the altered subcellular localization of the two proteins, but the constitutively activated form (Ser was mutated to Asp) of GhDi19-1/-2 still showed the nuclear localization. GhDi19-1/-2 overexpression transgenic Arabidopsis seedlings displayed the hypersensitivity to high salinity and abscisic acid (ABA). However, Ser site-mutated GhDi19-1(S116A) and GhDi19-2(S114A) and Ser and Thr double sites-mutated GhDi19-1(S/T-A/A) and GhDi19-2(S/T-A/A) transgenic Arabidopsis did not show the salt- and ABA-hypersensitive phenotypes. In contrast, overexpression of Thr site-mutated GhDi19-1(T114A) and GhDi19-2(T112A) in Arabidopsis still resulted in salt- and ABA-hypersensitivity phenotypes, like GhDi19-1/-2 transgenic lines. Overexpression of GhDi19-1/-2 and their constitutively activated forms in Atcpk11 background could recover the salt- and ABA-insensitive phenotype of the mutant. Thus, our results demonstrated that Ser phosphorylation (not Thr phosphorylation) is crucial for functionally activating GhDi19-1/-2 in response to salt stress and ABA signaling during early plant development and GhDi19-1/-2 proteins may be downstream targets of CDPKs in ABA signal pathway.
Diode‐switched thermal‐transfer printed antenna on flexible substrate
Authors: Kgwadi M. , Drysdale T.D. 
Published: February 2016

Diode‐switching that can be used to introduce frequency agility into antennas produced by thermal transfer printing is demonstrated. The particular example is a triangular Sierpinski fractal pattern with two PIN diodes to switch between operation optimised for the 800 MHz ultra‐high frequency (UHF) band (diodes on) and 2400 MHz ISM band (diodes off). Our measured results show an improvement in S11 in the UHF band from −2 to −28 dB and from −7 to −30 dB at 2400 MHz, when switching the diodes appropriately. The measured bandwidth is 200 (1000) MHz, and the measured directivity is 3.1 dB (5.2 dB) while the measured gain is −5.2 dB (6.7 dB) for the diodes on (off).
Ginger hexane extract suppresses RANKL-induced osteoclast differentiation
Authors: Ito Suguru , Ohmi Akihiro , Sakamiya Akiyo , Yano Takeo , Okumura Katsuzumi , Nishimura Norihiro , Kagontani Kazuhiro 
Published: February 2016

Abstract
Osteoporosis is a debilitating disease caused by decreased bone density. Compounds with anti-osteoclastic activity, such as bisphosphonates, may help in the prevention and treatment of osteoporosis. Herein, we determined the inhibitory effects of ginger hexane extract (GHE) on receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. The results showed that GHE (1) suppressed osteoclast differentiation and the formation of actin rings; (2) inhibited the expression of Nfatc1, a master transcriptional factor for osteoclast differentiation, in a dose-dependent manner (10–20 μg/mL); and (3) inhibited other osteoclastogenesis-related genes, such as Oscar, Dc-stamp, Trap, and Mmp9. These findings suggest that GHE may be used to prevent and treat osteoporosis by inhibiting osteoclast differentiation.
Unexplained Exertional Dyspnea Caused by Low Ventricular Filling Pressures: Results from Clinical Invasive Cardiopulmonary Exercise Testing
Authors: Oldham William M. , Lewis Gregory D. , Opotowsky Alexander R. , Waxman Aaron B. , Systrom David M. 
Published: February 2016

To determine whether low ventricular filling pressures are a clinically relevant etiology of unexplained dyspnea on exertion, a database of 619 consecutive, clinically indicated invasive cardiopulmonary exercise tests (iCPETs) was reviewed to identify patients with low maximum aerobic capacity (o2max) due to inadequate peak cardiac output (Qtmax) with normal biventricular ejection fractions and without pulmonary hypertension (impaired: n = 49, o2max = 53% predicted [interquartile range (IQR): 47%–64%], Qtmax = 72% predicted [62%–76%]). These were compared to patients with a normal exercise response (normal: n = 28, o2max = 86% predicted [84%–97%], Qtmax = 108% predicted [97%–115%]). Before exercise, all patients received up to 2 L of intravenous normal saline to target an upright pulmonary capillary wedge pressure (PCWP) of ≥5 mmHg. Despite this treatment, biventricular filling pressures at peak exercise were lower in the impaired group than in the normal group (right atrial pressure [RAP]: 6 [IQR: 5–8] vs. 9 [7–10] mmHg, P = 0.004; PCWP: 12 [10–16] vs. 17 [14–19] mmHg, P < 0.001), associated with decreased stroke volume (SV) augmentation with exercise (+13 ± 10 [standard deviation (SD)] vs. +18 ± 10 mL/m2, P = 0.014). A review of hemodynamic data from 23 patients with low RAP on an initial iCPET who underwent a second iCPET after saline infusion (2.0 ± 0.5 L) demonstrated that 16 of 23 patients responded with increases in Qtmax ([+24% predicted [IQR: 14%–34%]), o2max (+10% predicted [7%–12%]), and maximum SV (+26% ± 17% [SD]). These data suggest that inadequate ventricular filling related to low venous pressure is a clinically relevant cause of exercise intolerance.
Oral Treprostinil for the Treatment of Pulmonary Arterial Hypertension in Patients Transitioned from Parenteral or Inhaled Prostacyclins: Case Series and Treatment Protocol
Authors: Coons James C. , Miller Taylor , Simon Marc A. , Ishizawar David C. , Mathier Michael A. 
Published: January 2016

Oral treprostinil (TRE) is a prostacylin approved for the management of pulmonary arterial hypertension (PAH). Few data exist to guide the use of oral TRE as a replacement for parenteral or inhaled prostacyclins. Therefore, the purpose of this report was to describe our experience with oral TRE to transition patients from parenteral or inhaled TRE. We describe a case series of patients admitted for a 4‐day hospital stay to transition from parenteral or inhaled TRE. Appropriate criteria for transition included stable patients with improved symptoms/functional capacity, patients who could not tolerate intravenous prostacyclin due to infection or subcutaneous prostacyclin due to pain, and patient preference for transition. The dosing protocol for transition is described. A total of 9 patients generally representative of a typical PAH demographic and background medical therapy were included. Patients were initiated at either 0.5 or 1 mg 3 times daily and discharged on a median dose of 8 mg 3 times daily. Our protocol resulted in 6 of 9 patients who successfully transitioned at a median follow‐up of 47 weeks. Two patients had to return to their previous prostacyclin therapy based on the presence of clinical worsening and adverse events (n = 1) and adverse events alone (n = 1). Another patient discontinued therapy due to plans for hospice care. Oral TRE may serve an important role in prostacyclin transitions in carefully selected, stable patients who receive background oral therapy for PAH.
On the Importance of Hydrodynamic Interactions in the Stepping Kinetics of Kinesin
Authors: Goldtzvik Yonathan , Zhang Zhechun , Thirumalai D. 
Published: January 2016

AbstractConventional kinesin walks by a hand-over-hand mechanism on the microtubule (MT) by taking ~ 8nmdiscrete steps, and consumes one ATP molecule per step. The time needed to complete a single step is on the order of twenty microseconds. We show, using simulations of a coarse-grained model of the complex containing the two motor heads, the MT, and the coiled coil that in order to obtain quantitative agreement with experiments for the stepping kinetics hydrodynamic interactions (HI) have to be included. In simulations without hydrodynamic interactions spanning nearly twentyμsnot a single step was completed in hundred trajectories. In sharp contrast, nearly 14% of the steps reached the target binding site within 6μswhen HI were included. Somewhat surprisingly, there are qualitative differences in the diffusion pathways in simulations with and without HI. The extent of movement of the trailing head of kinesin on the MT during the diffusion stage of stepping is considerably greater in simulations with HI than in those without HI. Our results suggest that inclusion of HI is crucial in the accurate description of motility of other motors as well.
Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis
Authors: Steinberg Julia , Ritchie Graham R. S. , Roumeliotis Theodoros I. , Jayasuriya Raveen L. , Brooks Roger A. , Binch Abbie L. A. , Shah Karan M. , Coyle Rachael , Pardo Mercedes , Le Maitre Christine L. , Ramos Yolande F. M. , Nelissen Rob G. H. H. , Meulenbelt Ingrid , McCaskie Andrew W. , Choudhary Jyoti S. , Mark Wilkinson J. , Zeggini Eleftheria 
Published: January 2016

ABSTRACTBackgroundOsteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning disease progression are incompletely understood, but can be characterized using recent advances in genomics technologies, as the relevant tissue is readily accessible at joint replacement surgery. Here we investigate genes and pathways that mark OA progression, combining genome-wide DNA methylation, RNA sequencing and quantitative proteomics in isolated primary chondrocytes from matched intact and degraded articular cartilage samples across twelve patients with OA undergoing knee replacement surgery.ResultsWe identify 49 genes differentially regulated between intact and degraded cartilage at multiple omics levels, 16 of which have not previously been implicated in OA progression. Using independent replication datasets, we replicate statistically significant signals and show that the direction of change is consistent for over 90% of differentially expressed genes and differentially methylated CpG probes. Three genes are differentially regulated across all 3 omics levels:AQP1,COL1A1andCLEC3B, and all three have evidence implicating them in OA through animal or cellular model studies. Integrated pathway analysis implicates the involvement of extracellular matrix degradation, collagen catabolism and angiogenesis in disease progression. All data from these experiments are freely available as a resource for the scientific community.ConclusionsThis work provides a first integrated view of the molecular landscape of human primary chondrocytes and identifies key molecular players in OA progression that replicate across independent datasets, with evidence for translational potential.
Real-time predictions of reservoir size and rebound time during antiretroviral therapy interruption trials for HIV
Authors: Hill Alison L. , Rosenbloom Daniel I.S. , Goldstein Edward , Hanhauser Emily , Kuritzkes Daniel R. , Siliciano Robert F. , Henrich Timothy J. 
Published: January 2016

AbstractMonitoring the efficacy of novel reservoir-reducing treatments for HIV is challenging. The limited ability to sample and quantify latent infection means that supervised antiretroviral therapy (ART) interruption studies are generally required. Here we introduce a set of mathematical and statistical modeling tools to aid in the design and interpretation of ART-interruption trials. We show how the likely size of the remaining reservoir can be updated in real-time as patients continue off treatment, by combining the output of laboratory assays with insights from models of reservoir dynamics and rebound. We design an optimal schedule for viral load sampling during interruption, whereby the frequency of follow-up can be decreased as patients continue off ART without rebound. While this scheme can minimize costs when the chance of rebound between visits is low, we find that the reservoir will be almost completely reseeded before rebound is detected unless sampling occurs at least every two weeks and the most sensitive viral load assays are used. We use simulated data to predict the clinical trial size needed to estimate treatment effects in the face of highly variable patient outcomes and imperfect reservoir assays. Our findings suggest that large numbers of patients – between 40 and 150 – will be necessary to reliably estimate the reservoir-reducing potential of a new therapy and to compare this across interventions. As an example, we apply these methods to the two “Boston patients”, recipients of allogeneic hematopoietic stem cell transplants who experienced large reductions in latent infection and underwent ART-interruption. We argue that the timing of viral rebound was not particularly surprising given the information available before treatment cessation. Additionally, we show how other clinical data can be used to estimate the relative contribution that remaining HIV+ cells in the recipient versus newly infected cells from the donor made to the residual reservoir that eventually caused rebound. Together, these tools will aid HIV researchers in the evaluating new potentially-curative strategies that target the latent reservoir.
Micro-Magellan: A flexible, open source acquisition software for high throughput biological light microscopy
Authors: Pinkard Henry , Stuurman Nico , Corbin Kaitlin , Vale Ronald , Krummel Matthew F. 
Published: January 2016

ABSTRACTWe demonstrate the capabilities of μMagellan: a flexible, open source microscopy software for reproducible high throughput imaging of biological samples across heterogeneous scales of space and time. μMagellan provides a simple user interface for exploration and automated imaging of non-cuboidal regions. By utilizing the hardware abstraction layer of μMagellan, μMagellan provides a powerful and extensible platform for imaging heterogeneous biological samples on a wide range of existing microscopes.
Plastid degeneration inTillandsia(Bromeliaceae) provides evidence about the origin of multilamellar bodies in plants
Authors: van Doorn Wouter G. , Papini Alessio 
Published: January 2016

AbstractVesicle-like structures containing several to numerous concentric membranes, called multilamellar bodies (MLBs), are present both in animal and plant cells. The origin of MLBs in animal cells has been elucidated partially, while that of plant MLBs is unknown. MLBs in plant cells are present in the cytoplasm, at the interface of cytoplasm and vacuole, and inside vacuoles. This suggests that they become transported from the cytoplasm to the vacuole. The function of plant MLBs thus seems transfer of cellular membranes to the vacuole. Although it is often impossible to discern whether they have a single or a double outer membrane, in some examples a double outer membrane is present. This might suggest autophagic/mitochondrial/plastidial origin. Membrane structures similar to those in MLBs have not been described, apparently, in mitochondria. By contrast, structures similar to MLBs are found in autophagous structures and in degenerating chloroplasts and other plastids. The data might suggest the hypothesis that plant MLBs derive from autophagous structures and/or from plastids.
An interactive virtual lighting maintenance environment for human factors evaluation
Authors: He Qichang , Qiu Shiguang , Fan Xiumin , Liu Keyan 
Published: January 2016

Purpose
– The paper aims to establish a virtual lighting maintenance environment (VLME), and to analyze the visibility-related human factors (HFs) during maintenance operations through interactive simulations.

Design/methodology/approach
– First, an accurate task lighting modeling method was developed, which includes lighting information modeling and illuminant parameters calibration. Then, the real-time interaction between the task lighting and three-dimensional virtual human was modeled. After that, the attenuation coefficient of visibility was determined. Also, the HFs’ analysis process in VLME was described in detail.

Findings
– A case study of power supply module replacement of radar equipment was performed in VLME. The HFs’ analysis demonstrated that the task lighting significantly affects the visibility, which causes indirect impact on posture comfort and operation safety.

Practical implications
– Through evaluating maintenance operation processing in lighting environment, engineers can better analyze and validate the maintainability design for complex equipment, and some potential ergonomics and safety issues can be found and dealt earlier.

Originality/value
– An VLME was built for interactive “human-in-loop” maintenance operation simulation, which can support HFs’ evaluation in lighting environment accurately and effectively.
Whither the accounting profession, accountants and accounting researchers? Commentary and projections
Authors: Guthrie James , Parker Lee D. 
Published: February 2016

Purpose
– This commentary reflects on possible disruptions for the accounting profession, accountants and academics in the next 25 years. The traditional and highly valued role of the accounting professional, accountants and academic scholarship is rapidly changing in an intensely networked and interdisciplinary world. The purpose of this paper is to provide a summary of AAAJ activities for 2015 and in the next few years, it also delivers a call to action to interdisciplinary accounting researchers to undertake innovative research that reflects on what these turbulent times mean for society, nations, organisations and individual accountants, practitioners and educators.

Design/methodology/approach
– The paper employs a literature-based analysis and critique to provide a critical reflection on current and future developments in academic accounting research.

Findings
– In highlighting disruption to traditional accounting practice and research the authors provide a foundation from which researchers should contemplate their motivation, informing theories and values to ensure that their academic endeavours make a contribution to practice, policy and a wider societal good.

Practical implications
– It is hoped the practical and research issues explored in this commentary will invoke more interdisciplinary perspectives on accounting and the accounting profession, and assist scholars to reflect on the challenges they face.

Originality/value
– Providing a forward looking vision on the important role of academic researchers, the authors urge colleagues not to limit their vision to the narrowness that is an increasing feature of North American economics-based accounting research and to not simply observe, but rather construct an enabling accounting that can benefit society more widely.
Critical success factors of adapting heritage buildings: an exploratory study
Authors: Dyson Kristy , Matthews Jane , Love Peter E.D. 
Published: January 2016

Purpose
– The loss of heritage buildings should be avoided as they provide a tangible example of a period of life that is now gone. Adaptive re-use enables buildings to be given a second life, enabling them to live on when they may have been previously underutilized. Changing the capacity, function or performance of underutilized buildings for a different purpose, or to suit new conditions, or making use of pre-existing structural elements has become necessary to preserve heritage buildings. The purpose of this paper is to identify the critical success factors (CSF) for the adaptive re-use of heritage buildings.

Design/methodology/approach
– Identification of CSF for adaptive re-use can provide asset owners, developers and key stakeholders with the knowledge needed to ensure a project is delivered successfully. Due to a lack of research in the area of CSF for heritage buildings, an exploratory approach was undertaken. Semi-structured interviews were undertaken with stakeholders to solicit their views as to CSFs that lead to the successful adaption of all heritage buildings that had been subjected to re-use program in Perth, Western Australia, were examined.

Findings
– Four CSFs were identified: research; matching function; function; and design and minimal change. It is proffered that by addressing the CSFs issues associated with latent conditions, building layout and commercial risk and uncertainty can be addressed. Yet, the Building Code of Australia will continue to be the most significant issue for owners/developers and project teams who embrace an adaptive re-use project.

Originality/value
– To date there has been limited research undertaken with regard to determining the CSF for heritage buildings that have been subjected to adaptive re-use. The work presented in this paper identifies the key CSFs that emerged from the stock of heritage building’s in Perth, WA. Further research is required to determine the validity of the CSFs, however, those identified provide a benchmark for further studies in this fertile area.
Revisiting the cataloging of free Internet resources at the University of Denver
Authors: Brown Christopher C. , Elzi Erin 
Published: January 2016

Purpose
– This paper aims to present updated statistics demonstrating the value of cataloging free Internet resources and the challenges of batch loading, vendor records, electronic resource modules and discovery tools, as an update to the 2008 paper in this journal

Design/methodology/approach
– Updates the statistics from the URL redirection system for tracking user access to freely available Web publications.

Findings
– With more projects and bibliographic records included within the scope of the project, users still find and use the links to outbound content. New technologies and management methods support the cataloging of free Web content, even if, at times, cataloging standards are compromised.

Originality/value
– Several studies have focused on US federal document clickthroughs from the library catalog, but this is the only study to exclusively track clickthroughs to freely available Web content.