bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of wavelengths of LED light-therapy on endothelial cells
Authors: Rohringer Sabrina , Holnthoner Wolfgang , Chaudary Sidrah , Slezak Paul , Priglinger Eleni , Strassl Martin , Pill Karoline , Mühleder Severin , Redl Heinz , Dungel Peter 
Published: August 2017

AbstractLow level light therapy receives increasing interest in the fields of tissue regeneration and wound healing. Severalin vivostudies demonstrated the positive effects of LLLT on angiogenesis. This study aimed to investigate the underlying propertiesin vitroby comparing the effects of light therapy by light emitting diodes of different wavelengths on endothelial cellsin vitro. Human umbilical vein endothelial cells were treated with either 475 nm, 516 nm or 635 nm light. Control cells were not illuminated. 2D proliferation was quantified by manual counting. HUVEC migration was analyzed by performing a 2D wound scratch assay and a 3D bead assay. The influence of LLLT on early vasculogenic events was determined in a 3D fibrin co-culture model with adipose-derived stem cells. Stimulation with both red and green pulsed LED light significantly increased HUVEC proliferation and 3D migration. Moreover, HUVEC showed increased 2D migration potential with green light stimulation. The treatment with blue light was ineffective. Several parameters showed that green light was even more potent to stimulate proliferation and migration of endothelial cells than clinically well-established red light therapy. Further studies have to focus on intracellular mechanisms induced by different wavelengths in order to optimize this promising therapy in tissue regeneration.
Ligand activation induces different conformational changes in CXCR3 receptor isoforms as evidenced by plasmon waveguide resonance (PWR)
Authors: Boyé K. , Billottet C. , Pujol N. , Alves I. D. , Bikfalvi A. 
Published: August 2017

AbstractThe chemokine receptor CXCR3 plays important roles in angiogenesis, inflammation and cancer. Activation studies and biological functions of CXCR3 are complex due to the presence of spliced isoforms. CXCR3-A is known as a pro-tumor receptor whereas CXCR3-B exhibits anti-tumor properties. Here, we focused on the conformational change of CXCR3-A and CXCR3-B after agonist or antagonist binding using Plasmon Waveguide Resonance (PWR). Agonist stimulation induced an anisotropic response with very distinct conformational changes for the two isoforms. The CXCR3 agonist bound CXCR3-A with higher affinity than CXCR3-B. Using various concentrations of SCH546738, a CXCR3 specific inhibitor, we demonstrated that low SCH546738 concentrations (≤1 nM) efficiently inhibited CXCR3-A but not CXCR3-B’s conformational change and activation. This was confirmed by both, biophysical and biological methods. Taken together, our study demonstrates differences in the behavior of CXCR3-A and CXCR3-B upon ligand activation and antagonist inhibition which may be of relevance for further studies aimed at specifically inhibiting the CXCR3A isoform.
Permanent modifications in silica produced by ion-induced high electronic excitation: experiments and atomistic simulations
Authors: Rivera Antonio , Olivares José , Prada Alejandro , Crespillo Miguel L. , Caturla María J. , Bringa Eduardo M. , Perlado José M. , Peña-Rodríguez Ovidio 
Published: August 2017

AbstractThe irradiation of silica with ions of specific energy larger than ~0.1 MeV/u produces very high electronic excitations that induce permanent changes in the physical, chemical and structural properties and give rise to defects (colour centres), responsible for the loss of sample transparency at specific bands. This type of irradiation leads to the generation of nanometer-sized tracks around the ion trajectory. In situ optical reflection measurements during systematic irradiation of silica samples allowed us to monitor the irradiation-induced compaction, whereas ex situ optical absorption measurements provide information on colour centre generation. In order to analyse the results, we have developed and validated an atomistic model able to quantitatively explain the experimental results. Thus, we are able to provide a consistent explanation for the size of the nanotracks, the velocity and thresholding effects for track formation, as well as, the colour centre yield per ion and the colour centre saturation density. In this work we will discuss the different processes involved in the permanent modification of silica: collective atomic motion, bond breaking, pressure-driven atom rearrangement and ultra-fast cooling. Despite the sudden lattice energy rise is the triggering and dominant step, all these processes are important for the final atomic configuration.
Physical and Pharmacokinetic Characterizations of trans-Resveratrol (t-Rev) Encapsulated with Self-Assembling Lecithin-based Mixed Polymeric Micelles (saLMPMs)
Authors: Li Tzu-Pin , Wong Wan-Ping , Chen Ling-Chun , Su Chia-Yu , Chen Lih-Geeng , Liu Der-Zen , Ho Hsiu-O , Sheu Ming-Thau 
Published: August 2017

AbstractThis study involved physical and pharmacokinetic characterizations of trans-resveratrol (t-Rev)-loaded saLMPMs which attempted to improve t-Rev’s pharmacokinetic profiles and bioavailability resolving hurdles limiting its potential health benefits. The optimal formulation consisted of t-Rev, lecithin, and Pluronic® P123 at 5:2:20 (t-Rev-loaded PP123 saLMPMs) provided mean particle size <200 nm, encapsulation efficiency >90%, and drug loading >15%. Compared to t-Rev solubilized with HP-β-CD, t-Rev-loaded PP123 saLMPMs enhanced t-Rev’s stability in PBS at RT, 4 °C, and 37 °C and in FBS at 37 °C, and retarded the in vitro release. Intravenous administration of t-Rev-loaded PP123 saLMPMs was able to enhance 40% absolute bioavailability and a greater portion of t-Rev was found to preferably distribute into peripheral compartment potentially establishing a therapeutic level at the targeted site. With oral administration, t-Rev-loaded LMPMs increases 2.17-fold absolute bioavailability and furnished a 3-h period of time in which the plasma concentration maintained above the desirable concentration for chemoprevention and accomplished a higher value of the dose-normalized area under the curve for potentially establishing an effective level at the target site. Therefore, intravenous and oral pharmacokinetic characteristics of t-Rev encapsulated with PP123 saLMPMs indicate that t-Rev can be translated into a clinically useful therapeutic agent.
Novel oscillator model with damping factor for plasmon induced transparency in waveguide systems
Authors: Zhao Mingzhuo , Li Hongjian , He Zhihui , Chen Zhiquan , Xu Hui , Zheng Mingfei 
Published: August 2017

AbstractWe introduce a novel two-oscillator model with damping factor to describe the plasmon induced transparency (PIT) in a bright-dark model plasmonic waveguide system. The damping factor γ in the model can be calculated from metal conductor damping factor γ
c
and dielectric damping factor γ
d
. We investigate the influence of geometry parameters and damping factor γ on transmission spectra as well as slow-light effects in the plasmonic waveguide system. We can find an obvious PIT phenomenon and realize a considerable slow-light effect in the double-cavities system. This work may provide guidance for optical switching and plasmon-based information processing.
Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows
Authors: Wang Ming , Sun Zhaolin , Yu Tian , Ding Fangrong , Li Ling , Wang Xi , Fu Mingbo , Wang Haiping , Huang Jinming , Li Ning , Dai Yunping 
Published: August 2017

AbstractHuman lactoferrin (hLF) is a valuable protein for pharmaceutical products and functional foods, and worldwide demand for this protein has steadily increased. However, large-scale recombinant human lactoferrin (rhLF) production using current animal bioreactor techniques is limited by the low expression of foreign proteins, the use of antibiotic resistance genes and the down-regulation of endogenous milk proteins. Here, we generated a herd of marker-free, hLF bacterial artificial chromosome (BAC) transgenic cloned cows, as confirmed by Polymerase chain reaction, Southern blot and Western blot analyses. These transgenic cloned cows produced rhLF in milk at concentrations of 4.5–13.6 g/L. Moreover, the total protein content of the milk was increased. Over two hundred transgenic cloned cows were propagated by multiple ovulation and embryo transfer (MOET). A total of 400–450 g of rhLF protein, which shows similar enzymatic activity to natural hLF in iron binding and release, can be purified on a large scale from >100 L of milk per day. Our results suggested that transgenic bovine mammary bioreactors have the potential for large-scale protein production.
Compact, wideband‐printed quasi‐Yagi antenna using spiral metamaterial resonators
Authors: Aeini M. , Jarchi S. , Faraji‐Dana R. 
Published: August 2017

A novel structure for size reduction and bandwidth enhancement of quasi‐Yagi antenna using spiral resonator (SR) metamaterials is presented. The basic resonance is produced through excitation of simple quasi‐Yagi antenna elements through a microstrip feedline while parasitic SR metamaterials augment bandwidth. The antenna is designed on an glass‐reinforced epoxy laminate sheets (FR4) substrate with a dielectric constant of 4.4 and a thickness of 1.6 mm. Compared to original printed quasi‐Yagi antennas, the size of the proposed quasi‐Yagi antenna is reduced by ∼53%. Simulation results show that the proposed antenna has the impedance characteristics with a frequency bandwidth of 1.43–3.97 GHz (94%) for return loss more than 10 dB and a gain of 3.4–5.2 dBi in the corresponding frequency range. The designed antenna presents an end‐fire radiation with a front‐to‐back ratio >10 dB. The antenna is fabricated, and measurement results demonstrate compactness and wide bandwidth of the antenna.
Enabling PZT bimorph actuator with passive polysilicon structure
Authors: Chen S.‐H. , Michael A. , Kwok C. Y. 
Published: August 2017

For the first time, a bimorph thin film lead zirconate titanate (PZT) micro‐actuator with thick polysilicon as a passive structural layer is reported. A simple micro‐cantilever actuator consisting of 1000 µm long and 250 µm wide with 4 µm thick polysilicon structure flanked by a piezoelectric active layer on the top and bottom side has been fabricated to demonstrate such novel bimorph configuration. The actuator displays a flat profile with only 2 µm of tip deflection which indicates a balanced stress distribution in the structure. It is driven alternatively in both directions, demonstrating its unique suitability for micro‐actuation mechanisms where a passive structural layer is required to enhance the mechanical performance and reliability of the system. The static and dynamic measurements show similar characteristics for the top and bottom actuation. The actuator has a measured resonance frequency of 6.3 kHz, and displacement voltage sensitivities of 17.9 and 25.6 nm/V in the upward and downward direction, respectively.
High‐bit‐rate SOA‐based optical network unit with low seeding power that uses feedback seeding scheme
Authors: Shaltami Farhat M , Ab‐Rahman Mohammed Syuhaimi , Hwang I‐Shyan , Swedan Abdlhameed Almabrok 
Published: August 2017

This study presents a method to improve the efficiency of semiconductor optical amplifier (SOA)‐based optical network units (ONUs). The method is based on the combination of remote seeding and self‐seeding schemes, which is referred to as the feedback seeding (FBS) scheme. In the proposed method, a centralised laser source provides a coherent light wave at the optical line terminal. At the ONU, a fibre Bragg grating is positioned to reflect a small percentage of the SOA output to increase seeding power. Consequently, the instauration issue is overcome by achieving coherent light power and a higher data rate. A colourless FBS‐ONU is also demonstrated in this research. Simulation results demonstrate that the proposed FBS scheme can operate sufficiently with a low seeding power from the central office. In the remote seeding scheme, sensitivity increases as seeding power increases. On the other hand, in the FBS scheme, sensitivity is high and constant at seeding power values of −30 dBm and above. In this study, sensitivity is −23 dBm. The results show that the proposed scheme provides significant improvement in bit error rate performance and optical signal to noise ratio.
Fungal Dihydroxynaphthalene-Melanin: Diversity-Oriented Biosynthesis through Enzymatic and Non-enzymatic Transformations
Authors: Müller Michael , Husain Syed 
Published: August 2017

Tetrahydroxynaphthalene reductase (T4HNR) from Magnaporthe grisea catalyzes the reduction of polyhydroxynaphthalenes, hydroxynaphthoquinones, and 1,4-diketones, with extensive ramifications for the biosynthesis of (shunt) metabolites related to 1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis. Hence, an extended model for DHN-melanin biosynthesis has been developed which is based on a screening hypothesis involving non-enzymatic transformations such as oxidations and tautomerism. This has led to the broadening of the functions of several short-chain dehydrogenases/reductases (SDRs) capable of reducing polyhydroxyanthracenes, polyhydroxynaphthalenes, and polyhydroxybenzenes. Our work, broadening the scope of enzymatic dearomatization reactions, provides access to the biocatalytic synthesis of a variety of natural and natural-like products. Furthermore, the results described in this account provide the basis for the identification of other SDRs amenable to reducing aromatic compounds, and thus enable the identification of biosynthetic gene clusters most likely involved in the biosynthesis of aromatic polyketides.1	Introduction2	Biosynthesis of 1,8-Dihydroxynaphthalene (DHN)3	Biosynthesis of Shunt Metabolites and the Origin of Molecular Diversity3.1	Role of Spontaneous Non-enzymatic Oxidations3.2	Role of T4HNR and T3HNR3.3	Role of Tautomerism in the Biosynthesis of (Shunt) Metabolites4	Extended Melanin Biosynthesis: A Screening Hypothesis5	Useful Outcomes of the Newly Identified Melanin Biosynthetic Pathway5.1	NADP+ Regeneration Using Lawsone as Mediator5.2	Anthrahydroquinone as an Intermediate in the Biosynthesis of Chrysophanol and Other Anthraquinone-Derived Products5.3	Combination of T3HNR and GDH To Access trans-Ketodiols5.4	Phloroglucinol Reductases (PGRs) To Dearomatize Monomeric Phenols6	Conclusion
Penile Reconstruction with Skin Grafts and Dermal Matrices: Indications and Management
Authors: Triana Junco Paloma , Dore Mariela , Nuñez Cerezo Vanesa , Jimenez Gomez Javier , Miguel Ferrero Miriam , Díaz González Mercedes , Lopez-Pereira Pedro , Lopez-Gutierrez Juan 
Published: August 2017

Introduction The penis eventually needs specific cutaneous coverage in the context of reconstructive procedures following trauma or congenital anomalies. Local flaps are the first choice but are not always available after multiple previous procedures. In these cases, skin graft and dermal matrices should be considered.
Materials and Methods This study was a retrospective review of the past 4 years of four patients with severe loss of penile shaft skin who underwent skin reconstruction. Dermal matrices and skin grafts were utilized. Dermal matrices were placed for a median of 4.5 weeks (3.0–6.0 weeks). The skin graft was harvested from the inner thigh region for split-thickness skin graft (STSG) and the inguinal region for full-thickness skin graft (FTSG).
Results The four patients presented with complete loss of skin in the penile shaft. One patient had a vesical exstrophy, one had a buried penis with only one corpus cavernosum, one had a wide congenital lymphedema of the genitalia, and one had a lack of skin following circumcision at home. They underwent reconstruction with three patients undergoing split-thickness skin graft; two dermal matrices; and one full-thickness graft, respectively, thereby achieving a good cosmetic and functional result. There were no complications, and all the patients successfully accepted the graft.
Conclusion Dermal matrices and skin grafts may serve as effective tools in the management of severe penile skin defects unable to be covered with local flaps.
Height Increment and Laboratory Profile of Boys Treated With Aromatase Inhibitors With or Without Growth Hormone
Authors: Pedrosa Ludmila , de Oliveira Joice , Thomé Paula , Kochi Cristiane , Damiani Durval , Longui Carlos 
Published: August 2017

AbstractAromatase inhibitors (AIs) have been used to recover height loss due to their capacity to delay growth plate closure. Long-term studies describing final heights are needed to determine the efficacy and safety profiles of these drugs for the treatment of impaired growth. This study aims to identify the therapeutic efficiency of AIs in improve growth and to describe potential adverse effects during treatment. Retrospective data analysis of 96 adolescents, among which 22 patients already attained near-final height, were followed at outpatient clinics of two referral centers. Patients were all in puberty and present idiopathic decrease in predicted adult height. Patients were treated with Anastrozole (ANZ: 1 mg/day) or Letrozole (LTZ: 2.5 mg/day) with/without recombinant human growth hormone (0.05 mg/kg/day) for 1.0 to 3.5 years (2.1±1.2 years). Height gain, body mass index, lipid, liver enzyme, gonadotropins and testosterone levels were described before and at the end of treatment. Predicted adult height (PAH) and NF height were compared with the TH. The height SDS (adjusted to bone age) significantly increased (p<0.05) in all groups [0.8±0.7 (ANZ), 0.7±0.7 (ANZ+GH), 0.3±0.5 (LTZ), and 1.2±0.8 (LTZ+GH)]; the latter group exhibited the highest increment of PAH and growth recovery to the TH (p<0.004). No significant side effects were observed. AI treatment, especially when used in association with GH was able to improve growth and the attainment of familial target height.
Evaluation of Proposed Speckled Peacock Bass Cichla temensis Introduction to Puerto Rico
Authors: Neal J. Wesley , Bies Jason M. , Fox Cynthia N. , Lilyestrom Craig G. 
Published: August 2017

Abstract
Speckled Peacock Bass Cichla temensis are being considered for introduction into Puerto Rico to provide sportfishing opportunities and biological control for invasive New World cichlids. The proposed introduction was evaluated by following the American Fisheries Society's recommended seven-step approach to introducing nonnative fish. This paper documents the evaluation process, which included a rapid risk screening approach using the Fish Invasiveness Screening Kit (FISK) version 2 and a series of controlled experiments on prey selection, agonistic behavior, and competition. The overall FISK score was 6 with a certainty factor of 0.84, placing the introduction in the medium-risk category (0–18) for invasiveness. Many of the attributes elevating this species to the medium-risk designation were the same attributes desired during species selection, including generalist feeding behavior and large body size, which are required for predation on New World cichlids. In controlled experiments, Speckled Peacock Bass consumed all cichlid species offered, suggesting that they may have potential as a biological control agent, although some selectivity in species and size was observed. Speckled Peacock Bass showed aggression toward conspecifics and other species in a mesocosm environment, but aggression generally decreased with time. There was no evidence of negative effects on Largemouth Bass Micropterus salmoides in terms of growth or condition via aggression or competition in controlled small-pond experiments. Although some downstream movement from receiving reservoirs would be possible, downstream habitats are unsuitable for establishment of this species. The greatest risk may be unauthorized movement by anglers to other reservoirs where this species is not desired. It is the conclusion of this evaluation that Speckled Peacock Bass will consume New World cichlids and that the risks of introduction are acceptably low. Thus, introduction may proceed on a limited scale with proper scientific evaluation and public outreach directed at reducing unauthorized translocation by anglers.
Received October 12, 2016; accepted June 19, 2017 Published online August 31, 2017
tmVar 2.0: integrating genomic variant information from literature with dbSNP and ClinVar for precision medicine
Authors: Wei Chih-Hsuan , Phan Lon , Feltz Juliana , Maiti Rama , Hefferon Tim , Lu Zhiyong 
Published: August 2017

Abstract

Motivation
Despite significant efforts in expert curation, clinical relevance about most of the 154 million dbSNP reference variants (RS) remains unknown. However, a wealth of knowledge about the variant biological function/disease impact is buried in unstructured literature data. Previous studies have attempted to harvest and unlock such information with text-mining techniques but are of limited use because their mutation extraction results are not standardized or integrated with curated data.

Results
We propose an automatic method to extract and normalize variant mentions to unique identifiers (dbSNP RSIDs). Our method, in benchmarking results, demonstrates a high F-measure of ∼90% and compared favorably to the state of the art. Next, we applied our approach to the entire PubMed and validated the results by verifying that each extracted variant-gene pair matched the dbSNP annotation based on mapped genomic position, and by analyzing variants curated in ClinVar. We then determined which text-mined variants and genes constituted novel discoveries. Our analysis reveals 41 889 RS numbers (associated with 9151 genes) not found in ClinVar. Moreover, we obtained a rich set worth further review: 12 462 rare variants (MAF ≤ 0.01) in 3849 genes which are presumed to be deleterious and not frequently found in the general population. To our knowledge, this is the first large-scale study to analyze and integrate text-mined variant data with curated knowledge in existing databases. Our results suggest that databases can be significantly enriched by text mining and that the combined information can greatly assist human efforts in evaluating/prioritizing variants in genomic research.

Availability and implementation
The tmVar 2.0 source code and corpus are freely available at https://www.ncbi.nlm.nih.gov/research/bionlp/Tools/tmvar/
Videodermoscopy and Curettage: The Value of Simple Procedures During Mohs Surgery
Authors: Dika Emi , Fanti Pier Alessandro , Christman Holly , Ravaioli Giulia Maria , Patrizi Annalisa 
Published: August 2017

BACKGROUND
Mohs surgery (MS) is regarded as one of the most efficient surgical procedures for the treatment of skin tumors.

OBJECTIVES
Delineating tumor margins accurately is important in limiting broad excisions during conventional surgery and in reducing the number of stages during Mohs procedure.

METHODS
This study aimed to evaluate the role of 2 simple procedures, curettage and videodermoscopy (VDS), in determining margins during MS for the treatment of basal cell carcinoma (BCC) of the head and neck.

RESULTS
The use of both procedures offered a good intraoperative margin definition and consequently small surgical defects in 197 patients affected by BCC. Recurrences in basal cell cancers, which included both primary and recurrent lesions, occurred less frequently than in the institution's previous experience, appearing in only 8 of 197 cases (approx. 4%).

CONCLUSION
In the authors' experience, both VDS and curettage were found advantageous in delineating tumor margins while treating BCC with MS.