bluuebunny/crossref_metadata_2025_split huggingface dataset:
BEX3 contributes to cisplatin chemoresistance in nasopharyngeal carcinoma
Authors: Gao Wei , Li John Zeng‐Hong , Chen Si‐Qi , Chu Chiao‐Yun , Chan Jimmy Yu‐Wai , Wong Thian‐Sze 
Published: January 2017

AbstractNasopharyngeal carcinoma (NPC) can develop cisplatin‐resistant phenotype. Research has revealed that enriched in cancer stem cell population is involved in developing cisplatin‐resistant phenotype. CD271 is a candidate stem cell maker in head and neck cancers. The CD receptor does not possess any enzymatic property. Signal transduction function of CD271 is mediated by the cellular receptor‐associated protein. Our data showed that Brain‐expressed X‐linked 3 (BEX3), a CD271 receptor‐associated protein, was overexpressed in NPC. BEX3 overexpression was a unique event in cancer developed in the head and neck regions, especially NPC. BEX3 expression was inducible by cisplatin in NPC. In cisplatin‐resistant NPC xenograft, treatment with nontoxic level of cisplatin led to a remarkable increase in BEX3 level. High BEX3 expression was accompanied with high octamer‐binding transcription factor 4 (OCT4) expression in cisplatin‐resistant NPC. To confirm the inducing role of BEX3 on OCT4 expression, we knockdown BEX3 using siRNA and compared the expression of OCT4 with mock transfectants. Suppressing BEX3 transcripts led to a significant reduction in OCT4. In addition, targeting BEX3 using shRNA could increase the sensitivity of NPC cells to cisplatin. In summary, our results indicated a unique functional role of BEX3 in mediating the sensitivity of NPC cells to cisplatin. Targeting or blocking BEX3 activity might be useful in reversing the cisplatin‐resistant phenotype in NPC.
The Maintenance of Traditional and Technological Forms of Post‐Adoption Contact
Authors: Greenhow Sarah , Hackett Simon , Jones Christine , Meins Elizabeth 
Published: January 2017

Openness in adoption practice now often includes post‐adoption contact with the adopted child's birth family. Traditionally, indirect and direct contact has been supported and mediated by professionals following the adoption of children from the public care system in the UK. However, more recently, the widespread growth in the use of digital technologies has made it possible for both adopted children and birth relatives to search and contact one another through the use of sites such as Facebook without professional support. This practice has been called ‘virtual contact’. Using data from interviews with 11 adoptive parents and six adopted young people, who had experienced virtual contact, it is suggested that virtual contact works well when it is successfully integrated with the maintenance of more traditional methods of contact but can present risks when introduced without prior contact. Implications for practice are discussed in terms of how virtual contact can become a positive addition to adoption practices by utilising integrated methods of openness through which adoptive relationships can be maintained. Copyright © 2017 John Wiley & Sons, Ltd.‘Use of digital technologies has made it possible for both adopted children and birth relatives to search and contact one another… without professional support’Key Practitioner MessagesTechnology, used to supplement existing relationships with birth relatives, can be successful within a climate ofintegrated opennesswhere traditional and technological methods of contact are used.Prior ‘traditional’ contact can assist in the development of relationships and boundaries before moving to virtual contact.‘Out of the blue’ virtual contact, without prior connections, can be challenging and present risks.Integrated openness may not work well for all individuals and it is necessary to assess the appropriateness and value of all relationships irrespective of the methods of contact.
Practical and General Manganese‐Catalyzed Carbonylative Coupling of Alkyl Iodides with Amides
Authors: Li Yahui , Zhu Fengxiang , Wang Zechao , Rabeah Jabor , Brückner Angelika , Wu Xiao‐Feng 
Published: January 2017

AbstractA selective manganese‐catalyzed carbonylative transformation of alkyl iodides and amides was developed. A variety of imides were prepared in moderate to good yields. Alkyl bromides could also be applied by in situ treatment with NaI to give the corresponding alkyl iodides. Notably, no additives or expensive ligands were required here. As the first example of the carbonylative coupling of alkyl iodides with amides, the simple reaction conditions and the advantages of a manganese catalyst make this new general procedure more attractive and practical than conventional techniques. Mechanistically, control experiments and electron paramagnetic resonance spectroscopy studies were also performed, and the radical nature of this new process was proven.
Time Series Extended Finite‐State Machine‐Based Relevance Vector Machine Multi‐Fault Prediction
Authors: Zhou Zi-Qian , Zhu Qun-Xiong , Xu Yuan 
Published: January 2017

AbstractFault prediction means to detect faults that can occur in the future. While most studies focus on predicting one fault at a time, multi‐fault prediction is more practical for industrial processes as multiple faults can cause much more damage than a single one. A time series extended finite‐state machine (TS‐EFSM)‐based relevance vector machine (RVM) approach is proposed for multi‐fault prediction. Time lags and correlation coefficients between the process variables and process states are determined. Then, a variable and a state dependence diagram based on the correlation coefficients is established with the EFSM. Furthermore, the RVM is applied to identify parameters for the sake of better prediction accuracy and shorter testing times. With the prediction parameters, faults can be predicted using the aforementioned TS‐EFSM state transitions.
Regioselectivity Change in the Organocatalytic Enantioselective (3+2) Cycloaddition with Nitrones through Cooperative Hydrogen‐Bonding Catalysis/Iminium Activation
Authors: Prieto Liher , Juste‐Navarro Veronica , Uria Uxue , Delso Ignacio , Reyes Efraim , Tejero Tomas , Carrillo Luisa , Merino Pedro , Vicario Jose L. 
Published: January 2017

AbstractThe reaction of nitrones with enals through iminium activation can be modulated by using cooperative hydrogen‐bonding catalysis to induce the participation of a nitrone ylide (C‐N‐C) instead of the classical C‐N‐O dipole. As a consequence, N‐hydroxypyrrolidines are obtained, rather than the expected isoxazolidines. The reaction proceeds smoothly and high enantioselectivities are observed in all cases. By using the appropriate substrate, polysubstituted pyrrolidines incorporating quaternary stereocenters can be efficiently prepared.
Synthesis and Oxidation of a Paddlewheel‐Shaped Rhodium/Antimony Complex Featuring Pyridine‐2‐Thiolate Ligands
Authors: Wächtler Erik , Oro Luis A. , Iglesias Manuel , Gerke Birgit , Pöttgen Rainer , Gericke Robert , Wagler Jörg 
Published: January 2017

AbstractThe paddlewheel‐shaped complex [Sb(μ‐pyS)4Rh]2 (1) (pyS−= 2‐S‐C5H4N−) was synthesized from [Rh(pyS)(cod)]2 (cod=1,5‐cyclooctadiene) and Sb(pyS)3. Upon oxidation with ONMe3, the complex [(μ‐O)Sb(μ‐pyS)3Rh(κ2‐pyS)]2 (2) is formed. Both 1 and 2 form dimers and feature short Rh−Sb bonds and bridging pyS ligands. 121Sb Mössbauer spectro‐ scopy and computational studies were employed to elucidate the Rh−Sb bonding in 1 and 2. Both covalent (Rh−Sb, X‐type Sb ligand) and dative (Rh→Sb, Z‐type; Rh←Sb L‐type Sb ligand) interactions have to be considered for the description of their bonding situations.
Triarylmethane Dyes for Artificial Repellent Cotton Fibers
Authors: Montagut Ana Maria , Gálvez Erik , Shafir Alexandr , Sebastián Rosa María , Vallribera Adelina 
Published: January 2017

AbstractFamilies of new hydrophobic and/or oleophobic triarylmethane dyes possessing long hydrocarbon or polyfluorinated chains have been prepared. When covalently grafted on to cotton fabric, these dyes give rise to a new type of colored superhydrophobic fibers.
Small Molecule Activation with N,NR‐MIC Platinum Complexes
Authors: Jin Hanpeng , Mück‐Lichtenfeld Christian , Hepp Alexander , Stephan Douglas W. , Hahn F. Ekkehardt 
Published: January 2017

AbstractThe reaction of platinum complex trans‐[1] bearing an N,NEt‐imidazolide ligand with bis(diphenylphosphino)ethane (dppe) or bis(dicyclohexylphosphino)ethane (dchpe) yields the dinuclear MIC complexes [2]I2 or the mononuclear MIC complex [3]I, respectively. Whereas dinuclear [2]I2 does not react with elemental hydrogen, the mononuclear complex [3]I splits elemental hydrogen under mild reaction conditions with formation of hydride complex [4]I and N‐ethylimidazole. Dinuclear complex [3]I activates CS2 with formation of complex [5]I featuring the CS2 molecule bound through the carbon atom to the MIC nitrogen atom and one sulfur atom coordinating to the platinum center.
Education and support needs in patients with head and neck cancer: A multi‐institutional survey
Authors: Jabbour Joe , Milross Chris , Sundaresan Puma , Ebrahimi Ardalan , Shepherd Heather L. , Dhillon Haryana M. , Morgan Gary , Ashford Bruce , Abdul‐Razak Muzib , Wong Eva , Veness Michael , Palme Carsten E. , Froggatt Cate , Cohen Ruben , Ekmejian Rafael , Tay Jessica , Roshan David , Clark Jonathan R. 
Published: January 2017

BACKGROUNDHead and neck cancer (HNC) encompasses a diverse group of tumors, and thus providing appropriate and tailored information to patients before, during, and after treatment is a challenge. The objective of the current study was to characterize the experience and unmet needs of patients with HNC with regard to information and support provision.METHODSA 28‐question, cross‐sectional survey was completed by patients treated for HNC at 1 of 4 institutions in New South Wales, Australia (Chris O'Brien Lifehouse and Liverpool, Westmead, and Wollongong hospitals). It consisted of the adapted Kessler Psychological Distress Scale and questions assessing information quality, quantity, and format.RESULTSA total of 597 patients responded. The mean age of the patients was 58 years (range, 21‐94 years) with 284 men and 313 women (1:1.1). The majority of patients reported information concerning the disease process (76%), prognosis (67%), and treatment (77%) was sufficient, and approximately 50% reporting having received little or no information regarding coping with stress and anxiety. A substantial percentage of patients reported receiving minimal information concerning psychosexual health (56%) or the availability of patient support groups (56%). The majority of patients preferred access to multiple modes of information delivery (72%), with the preferred modality being one‐on‐one meetings with a health educator (37%) followed by internet‐based written information (19%).CONCLUSIONSPatients with HNC are a diverse group, with complex educational and support needs. Patients appear to be given information regarding survivorship topics such as psychological well‐being, patient support groups, and psychosexual health less frequently than information concerning disease and treatment. Verbal communication needs to be reinforced by accessible, well‐constructed, written and multimedia resources appropriate to the patient's educational level. Cancer 2017;123:1949–1957. © 2017 American Cancer Society.
Assessment of Telomere Length, Phenotype, and DNA Content
Authors: Kelesidis Theodoros , Schmid Ingrid 
Published: January 2017

AbstractTelomere sequences at the end of chromosomes control somatic cell division; therefore, telomere length in a given cell population provides information about its replication potential. This unit describes a method for flow cytometric measurement of telomere length in subpopulations using fluorescence in situ hybridization of fluorescently‐labeled probes (Flow‐FISH) without prior cell separation. After cells are stained for surface immunofluorescence, antigen‐antibody complexes are covalently cross‐linked onto cell membranes before FISH with a telomere‐specific probe. Cells with long telomeres are included as internal standards. Addition of a DNA dye permits exclusion of proliferating cells during data analysis. DNA ploidy measurements of cells of interest and internal standard are performed on separate aliquots in parallel to Flow‐FISH. Telomere fluorescence of G0/1 cells of subpopulations and internal standards obtained from Flow‐FISH are normalized for DNA ploidy, and telomere length in subsets of interest is expressed as a fraction of the internal standard telomere length. © 2017 by John Wiley & Sons, Inc.
Detecting APC Gene Mutations in Familial Adenomatous Polyposis (FAP)
Authors: Nallamilli Babi Ramesh Reddy , Hegde Madhuri 
Published: January 2017

AbstractHereditary forms of colorectal cancer (CRC) account for up to 5% of total cases. Familial adenomatous polyposis (FAP) is an autosomal dominant condition affecting nearly 1 in 5000 people and accounts for only about 1% of all CRCs. It is characterized by the progressive development of hundreds to thousands of adenomatous colon polyps. The gene associated with FAP (APC) contains 15 coding exons. The mutation spectrum of the APC gene is broad in that 87% of causative mutations are point mutations (including other sequence variants) and around 10% to 15% are intragenic deletions and duplications. The strategy for molecular diagnostic testing for FAP involves initial full sequence analysis of APC for sequence variants followed by screening for deletion/duplications using microarray‐based comparative genomic hybridization (array CGH) or Multiplex Ligation‐dependent Probe Amplification (MLPA). Recently, next generation sequencing (NGS)‐based targeted gene analysis has become clinically available for detection of point mutations and other sequence variants. This unit discusses detailed protocols for an NGS‐based sequencing assay, PCR‐based Sanger sequencing, and array CGH. © 2017 by John Wiley & Sons, Inc.
Assay for Transposase‐Accessible Chromatin Using Sequencing (ATAC‐seq) Data Analysis
Authors: Miskimen Kristy L.S. , Chan E. Ricky , Haines Jonathan L. 
Published: January 2017

AbstractThe study of epigenetic properties of the human genome, including structural modifications of DNA and chromatin, has increased tremendously as mounting evidence has demonstrated how much epigenetics affects human gene expression. Buenrostro et al. have developed a rapid method, requiring low numbers of living cells as input, for examining chromatin accessibility across the epigenome, known as the assay for transposase‐accessible chromatin using sequencing (ATAC‐seq). The overall goal of this unit is to provide a thorough ATAC‐seq data analysis plan, as well as describe how primary human blood samples can be processed for use in ATAC‐seq. In addition, a number of quality control parameters are discussed to ensure the integrity and confidence in the ATAC‐seq data. © 2017 by John Wiley & Sons, Inc.
Scarless Cas9 Assisted Recombineering (no‐SCAR) in Escherichia coli, an Easy‐to‐Use System for Genome Editing
Authors: Reisch Christopher R. , Prather Kristala L.J. 
Published: January 2017

AbstractThe discovery and development of genome editing systems that leverage the site‐specific DNA endonuclease system CRISPR/Cas9 has fundamentally changed the ease and speed of genome editing in many organisms. In eukaryotes, the CRISPR/Cas9 system utilizes a “guide” RNA to enable the Cas9 nuclease to make a double‐strand break at a particular genome locus, which is repaired by non‐homologous end joining (NHEJ) repair enzymes, often generating random mutations in the process. A specific alteration of the target genome can also be generated by supplying a DNA template in vivo with a desired mutation, which is incorporated by homology‐directed repair. However, E. coli lacks robust systems for double‐strand break repair. Thus, in contrast to eukaryotes, targeting E. coli chromosomal DNA with Cas9 causes cell death. However, Cas9‐mediated killing of bacteria can be exploited to select against cells with a specified genotype within a mixed population. In combination with the well described λ‐Red system for recombination in E. coli, we created a highly efficient system for marker‐free and scarless genome editing. © 2017 by John Wiley & Sons, Inc.
Mammalian Cell Tissue Culture Techniques
Authors: Phelan Katy , May Kristin M. 
Published: January 2017

AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2017 by John Wiley & Sons, Inc.
Preparation of Cell Cultures and Vaccinia Virus Stocks
Authors: Cotter Catherine A. , Earl Patricia L. , Wyatt Linda S. , Moss Bernard 
Published: January 2017

AbstractThe culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range‐restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.
The Five‐Choice Continuous Performance Task (5C‐CPT): A Cross‐Species Relevant Paradigm for Assessment of Vigilance and Response Inhibition in Rodents
Authors: Cope Zackary A. , Young Jared W. 
Published: January 2017

AbstractDeficits in the domains of attention and response inhibition are central to many psychiatric disorders. As such, animal models of disorders purporting to replicate these behavioral deficits first require tests that can accurately assess the behaviors with high fidelity. The gold‐standard clinical test of attention and response inhibition is the continuous performance test (CPT). Although there are a number of CPTs, all share the premise of responding to target stimuli and inhibiting from responding to non‐target stimuli. The recently developed rodent five‐choice CPT (5C‐CPT) requires similar behavioral responses, enabling signal detection parameter calculations. With demonstrable feasibility for rodent testing, the 5C‐CPT permits/facilitates: (1) delineation of neural mechanisms underlying these behaviors; (2) multifactorial analyses of the complex interplay between genetic and environmental manipulations relevant to psychiatric disorders; and hence (3) development of novel targeted treatments. All data to date indicate that the rodent 5C‐CPT described here has direct translatability to clinical CPTs, producing equivalent measures of behavior in experimental animals to those assessed in humans. The 5C‐CPT task provides an important tool toward delineating these mechanisms and developing treatments. However, it is also complex, with long training times and nuances requiring a thorough understanding before utilization. This unit will enable researchers to avoid potential missteps, greatly increasing the likelihood of success. © 2017 by John Wiley & Sons, Inc.
Sex‐specific responses to territorial intrusions in a communication network: Evidence from radio‐tagged great tits
Authors: Snijders Lysanne , van Oers Kees , Naguib Marc 
Published: January 2017

AbstractSignals play a key role in the ecology and evolution of animal populations, influencing processes such as sexual selection and conflict resolution. In many species, sexually selected signals have a dual function: attracting mates and repelling rivals. Yet, to what extent males and females under natural conditions differentially respond to such signals remains poorly understood, due to a lack of field studies that simultaneously track both sexes. Using a novel spatial tracking system, we tested whether or not the spatial behavior of male and female great tits (Parus major) changes in relation to the vocal response of a territorial male neighbor to an intruder. We tracked the spatial behavior of male and female great tits (N = 44), 1 hr before and 1 hr after simulating territory intrusions, employing automatized Encounternet radio‐tracking technology. We recorded the spatial and vocal response of the challenged males and quantified attraction and repulsion of neighboring males and females to the intrusion site. We additionally quantified the direct proximity network of the challenged male. The strength of a male's vocal response to an intruder induced sex‐dependent movements in the neighborhood, via female attraction and male repulsion. Stronger vocal responders were older and in better body condition. The proximity networks of the male vocal responders, including the number of sex‐dependent connections and average time spent with connections, however, did not change directly following the intrusion. The effects on neighbor movements suggest that the strength of a male's vocal response can provide relevant social information to both the males and the females in the neighborhood, resulting in both sexes adjusting their spatial behavior in contrasting ways, while the social proximity network remained stable. This study underlines the importance of “silent” eavesdroppers within communication networks for studying the dual functioning and evolution of sexually selected signals.
Geographical differentiation of the Euchiloglanis fish complex (Teleostei: Siluriformes) in the Hengduan Mountain Region, China: Phylogeographic evidence of altered drainage patterns
Authors: Li Yanping , Ludwig Arne , Peng Zuogang 
Published: January 2017

AbstractThe uplift of the Tibetan Plateau caused significant ecogeographical changes that had a major impact on the exchange and isolation of regional fauna and flora. Furthermore, Pleistocene glacial oscillations were linked to temporal large‐scale landmass and drainage system reconfigurations near the Hengduan Mountain Region and might have facilitated speciation and promoted biodiversity in southwestern China. However, strong biotic evidence supporting this role is lacking. Here, we use the Euchiloglanis fish species complex as a model to demonstrate the compound effects of the Tibetan Plateau uplift and Pleistocene glacial oscillations on species formation in this region. The genetic structure and geographical differentiation of the Euchiloglanis complex in four river systems within the Hengduan Mountain Region were deduced using the cytochrome b (cyt b) gene and 10 microsatellite loci from 360 to 192 individuals, respectively. The results indicated that the populations were divided into four independently evolving lineages, in which the populations from the Qingyi River and Jinsha River formed two sub‐lineages. Phylogenetic relationships were structured by geographical isolation, especially near drainage systems. Divergence time estimation analyses showed that the Euchiloglanis complex diverged from its sister clade Pareuchiloglanis sinensis at around 1.3 Million years ago (Ma). Within the Euchiloglanis complex, the divergence time between the Dadu–Yalong and Jinsha–Qingyi River populations occurred at 1.0 Ma. This divergence time was in concordance with recent geological events, including the Kun‐Huang Movement (1.2–0.6 Ma) and the lag time (<2.0 Ma) of river incision in the Hengduan Mountain Region. Population expansion signals were detected from mismatched distribution analyses, and the expansion times were concurrent with Pleistocene glacier fluctuations. Therefore, current phylogeographic patterns of the Euchiloglanis fish complex in the Hengduan Mountain Region were influenced by the uplift event of the Tibetan Plateau and were subsequently altered by paleo‐river transitions during the late Pleistocene glacial oscillations.