bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcription factors NRF2 and HSF1 have opposing functions in autophagy
Authors: Dayalan Naidu Sharadha , Dikovskaya Dina , Gaurilcikaite Egle , Knatko Elena V. , Healy Zachary R. , Mohan Hema , Koh Glenn , Laurell Axel , Ball Graeme , Olagnier David , de la Vega Laureano , Ganley Ian G. , Talalay Paul , Dinkova-Kostova Albena T. 
Published: September 2017

AbstractAutophagy plays a critical role in the maintenance of cellular homeostasis by degrading proteins, lipids and organelles. Autophagy is activated in response to stress, but its regulation in the context of other stress response pathways, such as those mediated by heat shock factor 1 (HSF1) and nuclear factor-erythroid 2 p45-related factor 2 (NRF2), is not well understood. We found that the Michael acceptor bis(2-hydoxybenzylidene)acetone (HBB2), a dual activator of NRF2 and HSF1, protects against the development of UV irradiation-mediated cutaneous squamous cell carcinoma in mice. We further show that HBB2 is an inducer of autophagy. In cells, HBB2 increases the levels of the autophagy-cargo protein p62/sequestosome 1, and the lipidated form of microtubule-associated protein light chain 3 isoform B. Activation of autophagy by HBB2 is impaired in NRF2-deficient cells, which have reduced autophagic flux and low basal and induced levels of p62. Conversely, HSF1-deficient cells have increased autophagic flux under both basal as well as HBB2-induced conditions, accompanied by increased p62 levels. Our findings suggest that NRF2 and HSF1 have opposing roles during autophagy, and illustrate the existence of tight mechanistic links between the cellular stress responses.
Dramatically Enhanced Superconductivity in Elemental Bismuth from Excitonic Fluctuation Exchange
Authors: Koley S. , Laad M. S. , Taraphder A. 
Published: September 2017

AbstractMotivated by the remarkable discovery of superconductivity in elemental Bismuth at ambient pressure, we study its normal state in detail using a combination of tight-binding (TB) band-structure supplemented by dynamical mean-field theory (DMFT). We show that a two-fluid model composed of preformed and dynamically fluctuating excitons coupled to a tiny number of carriers provides a unified rationalization of a range of ill-understood normal state spectral and transport data. Based on these, we propose that resonant scattering involving a very low density of renormalized carriers and the excitonic liquid drives logarithmic enhancement of vertex corrections, boosting superconductivity in Bi. A confirmatory test for our proposal would be the experimental verification of an excitonic semiconductor with electronic nematicity as a ‘competing order’ on inducing a semi-metal-to semiconductor transition in Bi by an external perturbation like pressure
Gene expression of NOX family members and their clinical significance in hepatocellular carcinoma
Authors: Eun Hyuk Soo , Cho Sang Yeon , Joo Jong Seok , Kang Sun Hyung , Moon Hee Seok , Lee Eaum Seok , Kim Seok Hyun , Lee Byung Seok 
Published: September 2017

AbstractNicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex-derived reactive oxygen species (ROS) promote chronic liver inflammation and remodeling that can drive hepatocellular carcinoma development. The role of NOX expression in hepatocellular carcinoma (HCC) has been partially investigated; however, the clinical relevance of collective or individual NOX family member expression for HCC survival remains unclear. Here, we obtained NOX mRNA expression data for 377 HCC samples and 21 normal liver controls from the TCGA data portal and performed Kaplan-Meier survival, gene ontology functional enrichment, and gene set enrichment analyses. Although most NOX genes exhibited little change, some were significantly induced in HCC compared to that in normal controls. In addition, HCC survival analyses indicated better overall survival in patients with high NOX4 and DUOX1 expression, whereas patients with high NOX1/2/5 expression showed poor prognoses. Gene-neighbour and gene set enrichment analyses revealed that NOX1/2/5 were strongly correlated with genes associated with cancer cell survival and metastasis, whereas increased NOX4 and DUOX1 expression was associated with genes that inhibit tumour progression. On the basis of these data, NOX family gene expression analysis could be a predictor of survival and identify putative therapeutic targets in HCC.
Neuropilin-1 is upregulated by Wnt/β-catenin signaling and is important for mammary stem cells
Authors: Liu Wei , Wu Ting , Dong Xiaobing , Zeng Yi Arial 
Published: September 2017

AbstractWnt/β-catenin signaling is instrumental for the development of mammary gland and the properties of mammary stem cells (MaSCs). The Wnt signaling downstream effectors that engage in regulating MaSCs have not been extensively studied. Here, we report that Neuropilin-1 (Nrp1) expression is induced by Wnt/β-catenin signaling in MaSCs, and its function is critical for the activity of MaSCs. Nrp1 is particularly expressed in MaSCs that are marked by the expression of Protein C Receptor (Procr). Knockdown of Nrp1 by shRNA diminishes MaSCs’ in vitro colony formation and in vivo mammary gland reconstitution ability. Similar results are seen when antagonizing Nrp1 using a dominant negative peptide. In genetic experiments, deletion of Nrp1 results in delay of mammary development. In addition, knockdown of Nrp1 inhibits MMTV-Wnt1 tumor growth in xenograft. Our data demonstrate that Nrp1 is critical for mammary development and tumorigenesis, revealing new insights into MaSC regulation and targeting stem cells in treatment of breast cancer.
Selective uptake of epidermal growth factor-conjugated gold nanoparticle (EGF-GNP) facilitates non-thermal plasma (NTP)-mediated cell death
Authors: Kim Wanil , Na Kyung-Yoon , Lee Kyung-Ha , Lee Hyun Wook , Lee Jae Koo , Kim Kyong-Tai 
Published: September 2017

AbstractNon-thermal atmospheric pressure plasma (NTP) has been shown to induce cell death in various mammalian cancer cells. Accumulated evidence also shows that NTP could be clinically used in cancer therapy. However, the current NTP-based applications lack target specificity. Here, a novel method in NTP-mediated cancer therapeutics was described with enhanced target specificity by treating EGF (epidermal growth factor)-conjugated GNP (gold nanoparticle). The treatment with EGF-conjugated GNP complex, followed by NTP irradiation showed selective apoptosis of cells having receptor-mediated endocytosis. NTP triggered γ–H2AX elevation which is a typical response elicited by DNA damage. These results suggest that EGF-conjugated GNP functions as an important adjuvant which gives target specificity in applications of conventional plasma therapy.
Nano-Integrated Suspended Polymeric Microfluidics (SPMF) Platform for Ultra-Sensitive Bio-Molecular Recognition of Bovine Growth Hormones
Authors: Sadabadi Hamid , Packirisamy Muthukumaran 
Published: September 2017

AbstractThe development of sensitive platforms for the detection of biomolecules recognition is an extremely important problem in clinical diagnostics. In microcantilever (MC) transducers, surface-stress is induced upon bimolecular interaction which is translated into MC deflection. This paper presents a cost-effective and ultra-sensitive MC-based biosensing platform. To address these goals, the need for costly high-resolution read-out system has been eliminated by reducing the cantilever compliance through developing a polymer-based cantilever. Furthermore a microfluidic system has been integrated with the MC in order to enhance sensitivity and response time and to reduce analytes consumption. Gold nanoparticles (AuNPs) are synthesized on the surface of suspended microfluidics as the selective layer for biomolecule immobilization. The biosensing results show significant improvement in the sensitivity of the proposed platform compared with available silicon MC biosensor. A detection limit of 2 ng/ml (100pM) is obtained for the detection of bovine growth hormones. The results validated successful application of suspended polymeric microfluidics (SPMF) as the next generation of biosensing platforms which could enable femtomolar (fM) biomolecular recognition detection.
TrkB-enhancer facilitates functional recovery after traumatic brain injury
Authors: Marshall John , Szmydynger-Chodobska Joanna , Rioult-Pedotti Mengia S. , Lau Kara , Chin Andrea T. , Kotla Siva K. Reddy , Tiwari Rakesh Kumar , Parang Keykavous , Threlkeld Steven W. , Chodobski Adam 
Published: September 2017

AbstractBrain-derived neurotrophic factor (BDNF), a key player in regulating synaptic strength and learning, is dysregulated following traumatic brain injury (TBI), suggesting that stimulation of BDNF signaling pathways may facilitate functional recovery. This study investigates whether CN2097, a peptidomimetic ligand which targets the synaptic scaffold protein, postsynaptic density protein 95, to enhance downstream signaling of tropomyosin-related kinase B, a receptor for BDNF, can improve neurological function after TBI. Moderate to severe TBI elicits neuroinflammation and c-Jun-N-terminal kinase (JNK) activation, which is associated with memory deficits. Here we demonstrate that CN2097 significantly reduces the post-traumatic synthesis of proinflammatory mediators and inhibits the post-traumatic activation of JNK in a rodent model of TBI. The recordings of field excitatory post-synaptic potentials in the hippocampal CA1 subfield demonstrate that TBI inhibits the expression of long-term potentiation (LTP) evoked by high-frequency stimulation of Schaffer collaterals, and that CN2097 attenuates this LTP impairment. Lastly, we demonstrate that CN2097 significantly improves the complex auditory processing deficits, which are impaired after injury. The multifunctionality of CN2097 strongly suggests that CN2097 could be highly efficacious in targeting complex secondary injury processes resulting from neurotrauma.
Preliminary evidence of olfactory signals of women’s fertility increasing social avoidance behavior towards women in pair-bonded men
Authors: Oren Chen , Shamay-Tsoory Simone G. 
Published: September 2017

AbstractPrevious studies suggest that women’s body odor is perceived as more attractive during ovulation and that exposure to women’s chemical signals of high fertility leads to increased mating motivation. Given that pair-bonded men react differently than single men to unfamiliar women, we investigated whether women’s chemical signals of fertility influence approach behavior among pair-bonded and single men. In the first experiment, men performed the Comfortable Interpersonal Distance task while exposed to body odor samples from women who were ovulating and from the same women during their luteal phase. We found that in the presence of the body odor from ovulation, pair-bonded, but not single men, maintained greater distance from different protagonists, particularly from women. In a second experiment we exposed men to women’s body odors while they rated the attractiveness and beauty of women’s faces. Although the ratings of women’s beauty did not differ across odor conditions, when the pair-bonded men were exposed to the high fertility odor they rated highly attractive women as less sexually attractive. The results suggest that exposure to fertility cues from unfamiliar women may trigger social avoidance in pair-bonded men, an outcome that may result from identifying such cues as threats to their relationship.
Graphene Improves the Biocompatibility of Polyacrylamide Hydrogels: 3D Polymeric Scaffolds for Neuronal Growth
Authors: Martín Cristina , Merino Sonia , González-Domínguez Jose M. , Rauti Rossana , Ballerini Laura , Prato Maurizio , Vázquez Ester 
Published: September 2017

AbstractIn tissue engineering strategies, the design of scaffolds based on nanostructures is a subject undergoing intense research: nanomaterials may affect the scaffolds properties, including their ability to interact with cells favouring cell growth and improving tissue performance. Hydrogels are synthetic materials widely used to obtain realistic tissue constructs, as they resemble living tissues. Here, different hydrogels with varying content of graphene, are synthesised by in situ radical polymerization of acrylamide in aqueous graphene dispersions. Hydrogels are characterised focusing on the contribution of the nanomaterial to the polymer network. Our results suggest that graphene is not a mere embedded nanomaterial within the hydrogels, rather it represents an intrinsic component of these networks, with a specific role in the emergence of these structures. Moreover, a hybrid hydrogel with a graphene concentration of only 0.2 mg mL−1 is used to support the growth of cultured brain cells and the development of synaptic activity, in view of exploiting these novel materials to engineer the neural interface of brain devices of the future. The main conclusion of this work is that graphene plays an important role in improving the biocompatibility of polyacrylamide hydrogels, allowing neuronal adhesion.
Pan-genome analysis of Bacillus for microbiome profiling
Authors: Kim Yihwan , Koh InSong , Young Lim Mi , Chung Won-Hyong , Rho Mina 
Published: September 2017

AbstractRecent advances in high-throughput sequencing technology allow for in-depth studies on microbial genomes and their communities. While multiple strains of the same species could display genomic variations with different gene contents in diverse habitats and hosts, the essential functions for a specific species are conserved as core genes that are shared among strains. We have comprehensively analyzed 238 strains of five different Bacillus species to identify the properties of core and strain-specific genes. Core and strain-specific genes in each Bacillus species show significant differences in their functions and genomic signatures. Using the core genes defined in this study, we have precisely identified the Bacillus species that exist in food microbiomes. Without resorting to culture-based whole genome sequencing, an unexpectedly large portion of the core genes, 98.22% of core genes in B. amyloliquefaciens and 97.77% of B. subtilis, were reconstructed from the microbiome. We have performed a pan-genome analysis on the core gene data of multiple Bacillus species to investigate the Bacillus species in food microbiome. Our findings provide a comprehensive genetic landscape of the Bacillus species, which is also consistent with previous studies on a limited number of strains and species. Analysis based on comprehensive core genes should thus serve as a powerful profiling tool to better understand major constituents in fermented food microbiomes.
Stretchable conductive elastomer for wireless wearable communication applications
Authors: Chen Zhibo , Xi Jingtian , Huang Wei , Yuen Matthew M. F. 
Published: September 2017

AbstractWearable devices have provided noninvasive and continuous monitoring of physiological parameters in healthcare applications. However, for the comfortable applications of wearable devices on human body, two key requirements are to replace conventional bulky devices into soft and deformable ones and to have wireless wearable communication. In this paper we present a simple, low-cost and highly efficient all-elastomeric conductor that can be used in a soft radio-frequency (RF) transmission line and antenna. We show a stretchable transmission line and two stretchable antennas fabricated with conventional screen printing. The stretchable conductor used in this fabrication method, which is a mixture of Ag and Polydimethylsiloxane (PDMS), can be stretched at high strains while maintaining a high conductivity, low attenuation and feasible radiation performance. The measured conductivity of the stretchable conductor reaches 1000 S/cm. Additionally, the highly conductive printed Ag-PDMS is utilized to construct transmission lines and antennas. The performance of these stretchable components, especially under different conditions of bending, stretching and twisting, are experimentally examined in common wireless-communication frequency bands. Our results demonstrate that printed Ag-PDMS enabled RF passive components have the desired property and quality for wireless wearable communication applications, which would provide new opportunities for wearable healthcare electronics.
Macrophages unlock progression of breast cancer cells experiencing matrigel-segregation in transplantation models
Authors: Ogura Misa , Bridgeman Victoria L. , Malanchi Ilaria 
Published: September 2017

AbstractBasement membrane matrix proteins, such as matrigel, are able to improve the efficiency of tumour transplantation. This assay represents the gold standard to measure tumour initiation potential in vivo of a limited number of cancer cells. However, in culture conditions, matrigel directly signals to cancer cells altering their phenotype. We here investigate how matrigel influences the tumour reconstitution dynamics of breast cancer cells in vivo. This is particularly relevant in the setting of limiting dilution assay where cells are transplanted in a relatively high amount of Matrigel. We show that matrigel initially induces a normalized growth of transplanted MMTV-PyMT breast tumours cells. This occurs in the context of a matrigel-segregation effect where cancer cells are transiently isolated from host tissue. We identify macrophages as gatekeepers of the cancer-host cell interaction: depriving transplants from macrophages locked cancer cells in this isolated environment where they fail to form tumours despite retaining their intrinsic tumorigenic potential. This is a decisive proof of concept that cancer cells’ malignant behaviour can be dominated by their microenvironment. Moreover, considering that diverse breast cancer cells are differently subjected to a segregation effect, this needs to be considered when comparing tumour initiation potential of different cancer cells.
Interaction between G-Quadruplex and Zinc Cationic Porphyrin: The Role of the Axial Water
Authors: Yao Xiangzi , Song Di , Qin Tingxiao , Yang Chunfan , Yu Ze , Li Xiaohong , Liu Kunhui , Su Hongmei 
Published: September 2017

AbstractThe interaction of ligands with G-quadruplexes has attracted considerable attention due to its importance in molecular recognition and anticancer drugs design. Here, we utilize triplet excited state as a sensitive reporter to study the binding interaction of zinc cationic porphyrin (ZnTMPyP4) with three G-quadruplexes, AG3(T2AG3)3, (G4T4G4)2, and (TG4T)4. By monitoring the triplet decay dynamics of ZnTMPyP4 with transient absorption spectroscopy, the coexisted binding modes via π-π stacking of porphyrin macrocycle and the G-quartets are allowed to be identified quantitatively, which involve intercalation (25% and 36%) versus end-stacking (75% and 64%) for AG3(T2AG3)3 and (G4T4G4)2, and end-stacking (23%) versus partial intercalation (77%) for (TG4T)4. It is shown that the steric hindrance of the axial water decreases greatly the percentage of intercalation. Further, a rapid assessment of binding stoichiometry is fulfilled by measuring the triplet decay dynamics under various [G-quadruplex]/[ZnTMPyP4] ratios. The binding stoichiometric ratios of G-quadruplex/ZnTMPyP4 are 1:2 for AG3(T2AG3)3, 1:1 for (G4T4G4)2, and 1:2 for (TG4T)4, which agree well with results obtained by the conventional method of continuous variation analysis. These results reveal a clear scenario of G-quadruplex/ZnTMPyP4 interaction and provide mechanistic insights for the application of anticancer drug designs using G-quadruplex as target.
Monitoring the colonization and infection of legume nodules by Micromonospora in co-inoculation experiments with rhizobia
Authors: Benito Patricia , Alonso-Vega Pablo , Aguado Carolina , Luján Rafael , Anzai Yojiro , Hirsch Ann M. , Trujillo Martha E. 
Published: September 2017

AbstractThe discovery that the actinobacterium Micromonospora inhabits nitrogen-fixing nodules raised questions as to its potential ecological role. The capacity of two Micromonospora strains to infect legumes other than their original host, Lupinus angustifolius, was investigated using Medicago and Trifolium as test plants. Compatible rhizobial strains were used for coinoculation of the plants because Micromonospora itself does not induce nodulation. Over 50% of nodules from each legume housed Micromonospora, and using 16S rRNA gene sequence identification, we verified that the reisolated strains corresponded to the microorganisms inoculated. Entry of the bacteria and colonization of the plant hosts were monitored using a GFP-tagged Lupac 08 mutant together with rhizobia, and by using immunogold labeling. Strain Lupac 08 was localized in plant tissues, confirming its capacity to enter and colonize all hosts. Based on studying three different plants, our results support a non-specific relationship between Micromonospora and legumes. Micromonospora Lupac 08, originally isolated from Lupinus re-enters root tissue, but only when coinoculated with the corresponding rhizobia. The ability of Micromonospora to infect and colonize different legume species and function as a potential plant-growth promoting bacterium is relevant because this microbe enhances the symbiosis without interfering with the host and its nodulating and nitrogen-fixing microbes.
Metformin Improves Endothelial Function and Reduces Blood Pressure in Diabetic Spontaneously Hypertensive Rats Independent from Glycemia Control: Comparison to Vildagliptin
Authors: Hamidi Shishavan Mahdi , Henning Robert H. , van Buiten Azuwerus , Goris Maaike , Deelman Leo E. , Buikema Hendrik 
Published: September 2017

AbstractMetformin confers vascular benefits beyond glycemia control, possibly via pleiotropic effects on endothelial function. In type-1-diabetes-mellitus (T1DM-)patients metformin improved flow-mediated dilation but also increased prostaglandin(PG)-F2α, a known endothelial-contracting factor. To explain this paradoxical finding we hypothesized that metformin increased endothelial-vasodilator mediators (e.g. NO and EDHF) to an even larger extent. Spontaneously-hypertensive-rats (SHR) display impaired endothelium-dependent relaxation (EDR) involving contractile PGs. EDR was studied in isolated SHR aortas and the involvement of PGs, NO and EDHF assessed. 12-week metformin 300 mg/kg/day improved EDR by up-regulation of NO and particularly EDHF; it also reduced blood pressure and increased plasma sulphide levels (a proxy for H2S, a possible mediator of EDHF). These effects persisted in SHR with streptozotocin (STZ)-induced T1DM. Vildagliptin (10 mg/kg/day), targeting the incretin axis by increasing GLP-1, also reduced blood pressure and improved EDR in SHR aortas, mainly via the inhibition of contractile PGs, but not in STZ-SHR. Neither metformin nor vildagliptin altered blood glucose or HbA1c. In conclusion, metformin reduced blood pressure and improved EDR in SHR aorta via up-regulation of NO and particularly EDHF, an effect that was independent from glycemia control and maintained during T1DM. A comparison to vildagliptin did not support effects of metformin mediated by GLP-1.