bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Adding the White Legged Shrimp (Penaeus vannamei) on Growth and Survival of Tiger Shrimp (Penaeus monodon) in Intensive Farming
Authors: Tarsim . 
Published: February 2017

<p>This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (<em>Penaeus vannamei</em>) on growth and survival of tiger shrimp (<em>Penaeus monodon</em>) in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield affected by addition of this species. Final affected by white legged shrimp addition. No adding effects were found on the water quality parameters. The results indicate that, at densities tested, white legged shrimp addition to the intensive farming of tiger shrimp polyculture is viable as species increase crop production</p> <p>Key words: <em>Penaeus vannamei</em>, <em>P. monodon</em>, growth, survival, polyculture</p>
White Shrimp (Litopenaeus vannamei) Production on Different Rearing Densities in Biocrete Pond
Authors: Budiardi T. , Muzaki A. , Utomo N.B.P. 
Published: February 2017

<p>Study on white shrimp (<em>Litopenaeus vannamei</em>) production in <em>biocrete</em> pond in different density was performed at PT. Bimasena Segara, Citarate, Sukabumi, West Java. Six ponds were used and devided into 2 groups based the density levels, i.e., 72-73 shrimp/m<sup>2</sup> and 93-105 shrimp/m<sup>2</sup>. Experiment was done for 100 days, including pond preparation. The results of study showed that mean weight of shrimp reared in biocrete pond was ranged from 9.64 to 12.34 gram, survival rate 26.97-99.58%, productivity 3,078-8,340.9 kg/ha, feed quantity 2,084.5-2,870.4 kg and FCR 1.19-2.60. Ponds with lower rearing density had lower (P<0.1) productivity, mean weight, survival and FCR compared to those of higher rearing density.</p> <p>Keywords: <em>Litopenaeus vannamei</em>, <em>biocrete</em> pond, rearing density</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian mengenai produksi udang vaname (<em>Litopenaeus vannamei</em>) di tambak <em>biocrete</em> dengan padat penebaran berbeda telah dilakukan di tambak PT. Bimasena Segara, Citarate, Sukabumi, Jawa Barat menggunakan 6 petak tambak yang dibedakan menjadi 2 kelompok berdasarkan padat tebar udang yang selanjutnya menjadi perlakuan, yaitu : 72-73 ekor/m<sup>2</sup> dan 93-105 ekor/m<sup>2</sup>. Penelitian ini dilakukan selama 100 hari termasuk persiapan tambak. Hasil penelitian menunjukkan abhwa bobot rata-rata udang yang dipelihara di tambak biocrete berkisar antara 9,64-12,34 g, kelangsungan hidup berkisar antara 26,97-99,58%, produktivitas berkisar antara 3078,7-8340,9 kg/ha, jumlah pakan yang diberikan berkisar antara 2084,5-2870,4 kg dan FCR berkisar antara 1,19-2,60. Petak dengan kepadatan rendah memiliki nilai produktivitas, bobot rata-rata, kelangsungan hidup dan konversi pakan (FCR) berbeda nyata (P < 0,1) dan lebih baik dibanding dengan yang berkepadatan tinggi.</p> <p>Kata kunci : <em>Litopenaeus vannamei</em>, tambak <em>biocrete</em>, padat tebar</p>
Potential of Chromolaena odorata Leaf as A Cure of Aeromonas hydrophila on Giant Gouramy (Osphronemus gouramy)
Authors: Hadiroseyani Y. , Hafifuddin . , Alifuddin M. , Supriyadi H. 
Published: February 2017

<p>This study was conducted to examine the potency of <em>Chromolaena odorata</em> leaf extract as a medicine for skin eruption disease caused by Aeromonas hydrophila in giant gouramy <em>Osphronemus gouramy</em>. Leaf extract of Chromolaena odorata for in vitro test was 0 (as control), 13000, 15000, 17000, 19000 and 21000 ppm, poured onto TSA medium containing bacteria 103 cfu/ml, and then is incubated for 24 hours. In vivo test was performed by injecting bacteria 0.1 ml of 10<sup>9</sup> cfu/ml intramuscularly into giant gouramy (14 g weight), and then fish were maintained in the water containing 15000 ppm of Chromolaena odorata leaf extract. In vitro study showed that prevention area of leaf extract against Aeromonas hydrophila was increase by increasing the concentration of leaf extract used, reached 9,33 mm. Prevention zone of leaf extract by difusion tends to constant, reached 7,6 mm. By in vivo test, survival rate of giant gouramy infected by <em>Aeromonas hydrophila</em> was no significantly different between dosages of leaf extract. All treated fish, excluded control died after 24 hours infection.</p> <p>Keywords: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui potensi daun kirinyuh <em>Chromolaena odorata</em> sebagai obat untuk penyakit cacar yang diakibatkan oleh <em>Aeromonas hydrophila</em> pada ikan gurame <em>Osphronemus gouramy</em>. Konsentrasi ekstrak daun <em>Chromolaena odorata</em> untuk uji <em>in vitro</em> adalah 13000, 15000, 17000, 19000 dan 21000 serta 0 ppm sebagai kontrol, yang diletakkan di atas media TSA yang telah mengandung biakan bakteri 103 cfu/ml dan diinkubasi selama 24 jam. Uji <em>in vivo</em> dilakukan dengan menginjeksikan bakteri sebanyak 0,1 ml (10<sup>9</sup> cfu/ml) secara intramuskular ke ikan gurame (berat 14 g) dan kemudian ikan dipelihara dalam air yang mengandung ekstrak daun kirinyuh 15000 ppm. Hasil uji <em>in virto</em> menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kirinyuh basah semakin efektif dalam menghambat perkembangan <em>A. hydrophila</em> dengan zona hambat tertinggi mencapai 9,33 mm. Zona hambat yang dihasilkan melalui metode difusi cenderung konstan, mencapai 7,6 mm. Melalui uji <em>in vivo</em>, tingkat kelangsungan hidup ikan gurame yang tidak berbeda nyata pada masing-masing perlakuan, bahkan terjadi kematian total dalam 24 jam pada semua perlakuan, kecuali kontrol.</p> <p>Kata kunci: <em>Aeromonas hydrophila</em>, <em>Osphronemus gouramy</em>, <em>Chromolaena odorata</em></p>
Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%
Authors: Junior M. Zairin , Handayani S. , Supriatna I. 
Published: February 2017

<p>Attempt to produce a lot number of <em>Tor soro</em> is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem. Cryopreservation of semen is one of sperm cryopreservation methods using liquid nitrogen (-196<sup>o</sup>C) to quickly freeze the sperm. Several cryoprotectants can be utilized in cryopreservation. In this study, quality by means of motility of cryopreserved sperm using dimethylsulfoxide or glycerol as cryoprotectant in the dose of 5, 10 and 15% were analyzed. The result of study show that percentage of sperm motile after cryopreservation using dimethylsulfoxide and glycerol was similar ranged from 76.7 to 83.3%. Therefore, both cryoprotectant used in this study could be employed for cryopreservation of Tor soro sperm.</p> <p>Keywords: cryopreservation, dimethylsulfoxide, glycerol, sperm, <em>Tor soro</em></p> <p><strong> </strong></p> <p><strong>ABSTRAK</strong></p> <p>Upaya produksi ikan batak (<em>Tor soro</em>) dalam jumlah besar relatif sulit jika hanya mengandalkan hasil pemijahan secara alami karena kematangan gonad antara ikan jantan dan betina sering tidak bersamaan. Untuk mengatasinya diperlukan stok sperma yang disimpan dalam jumlah yang cukup banyak untuk pembuahan yang dapat dipakai setiap saat. Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196°C) untuk membekukan sperma secara cepat. Beberapa jenis protektan dapat digunakan dalam pembekuan sperma. Pada penelitian ini, dilakukan analisis kualitas dalam arti motilitas sperma hasil kriopreservasi menggunakan dimetilsulfoksida dan gliserol sebagai krioprotektan dengan dosis 5, 10 dan 15%. Hasil penelitian menunjukkan bahwa persentase sperma motil setelah dilakukan kriopreservasi adalah sama, berkisar antara 76,7% hingga 83,35%. Dengan demikian, kedua jenis krioprotektan yang digunakan dalam penelitian ini bisa dipakai dalam kriopreservasi sperma ikan batak.</p> <p>Kata kunci: kriopreservasi, dimetilsulfoksida, gliserol, sperma, <em>Tor soro</em></p>
Effect injection of Ginger Extract on Development and Nucleus position of “Sangkuriang” Catfish Clarias sp. eggs
Authors: Junior M. Zairin , Yustikasari Y. , Arfah H. 
Published: February 2017

<p>One of methods could be applied to continuously meet the need of fish fry including catfish (<em>Clarias </em>sp.) is artificial propagation by inducing ovulation and spawning. As an alternative of existing method, in this study, ginger extract was intramuscularly injected to induce development of catfish eggs. Ginger is known as an important regulator of the balance of arachidonat cycle. The dose of ginger extract injected was 0, 0.5, 1.0 and 1.5 mL/kg broodstock. The results of study showed that injection 100% of ginger extracts in all doses was insignificantly inducing development of egg diameter and its nucleus position of catfish. Other chemicals exist in ginger extract might be functions as an obstacle for egg development of catfish.</p> <p>Keywords: catfish, <em>Clarias</em> sp., ginger, ovulation, egg</p> <p> </p> <p>ABSTRAK</p> <p>Salah satu cara untuk memenuhi kebutuhan benih ikan termasuk lele (<em>Clarias </em>sp.) secara kontinyu adalah penggunaan teknologi pembiakan buatan melalui perangsangan ovulasi dan pemijahan. Sebagai alternatif teknik yang sudah ada, dicobakan perangsangan perkembangan telur ikan lele menggunakan bahan ekstrak jahe yang dilakukan melalui penyuntikan secara intramuskular. Jahe telah dikenal sebagai suatu pengatur penting atas keseimbangan siklus arakidonat. Dosis ekstrak jahe yang disuntikkan adalah 0, 0,5, 1 dan 1,5 mL/kg induk. Penyuntikan 100% ekstrak jahe pada semua dosis perlakuan belum dapat merangsang perkembangan diameter dan posisi inti sel telur ikan lele sangkuriang. Adanya bahan lain yang terdapat pada jahe diduga sebagai penghambat bagi perkembangan telur ikan lele.</p> <p>Kata kunci: Ikan lele, <em>Clarias</em> sp., jahe, ovulasi, sel telur</p>
Effect of Ultraviolet Radiation on Pathogenity of White Spot Syndrome Virus in Giant Tiger Prawn (Penaeus monodon Fab.)
Authors: Subkhan M. , Alifuddin M. , Taslihan A. 
Published: February 2017

<p>This experiment was conducted to examine the effect of UV irradiation on pathogenity of white spot syndrome virus (WSSV) in black tiger shrimp (<em>Penaeus monodon</em> Fab.). A hundred ml of WSSV virus suspension (200 μg/ml) were placed at 30 cm under UV light 10 Watt. Radiation on WSSV virus was performed for 15, 30, 45, and 60 minutes. Black tiger sjrimp in density of 260 tails/L were immersed in 1000 ml of irradiated virus suspension (20 μg/ml) to test their pathogenities. The results of study showed that duration of UV irradiation on WSSV virus was reverse correlated to their pathogenities. In constrast, survival of black tiger shrimp was linear correlated to duration of UV inactivation of virus. Higher survival rate of shrimp (65.52%) after challenge test was obtained by irradiation of virus for 60 min.</p> <p>Keywords: WSSV, virus, pathogen, ultraviolet, black tiger prawn, <em>Penaeus monodon</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui pengaruh radiasi UV terhadap patogenitas virus <em>White Spot Syndrome Virus</em> (WSSV) pada udang windu (<em>Penaeus monodon </em>Fab). Suspensi virus WSSV dengan konsentrasi 200 μg/ml sebanyak 100 ml ditempatkan 30 cm diradiasi menggunakan UV 10 Watt. Radiasi dilakukan selama 15, 30, 45, dan 60 menit. Udang dengan kepadatan 260 ekor/L direndam dalam 1000 ml suspensi virus (20 μg/ml) hasil radiasi untuk menguji patogenitasnya. Hasil penelitian menunjukkan bahwa lama radiasi UV pada virus WSSV berbanding terbalik dengan tingkat patogenitasnya. Sementara itu, lama inaktivasi virus dengan UV berbanding lurus terhadap kelangsungan hidup udang. Kelangsungan hidup udang windu tertinggi (65,52%) setelah uji tantang diperoleh dengan meradiasi virus selama 60 menit.</p> <p>Kata kunci: WSSV, virus, patogen, ultraviolet, udang windu, <em>Penaeus monodon</em></p>
Oxygen Consumption of White Shrimp (Litopenaeus vannamei) and Model of Oxygen Management in Intensive Culture Pond
Authors: Budiardi T. , Batara T. , Wahjuningrum D. 
Published: February 2017

<p>This study was conducted to determine oxygen consumption level of white shrimp (<em>Litopenaeus vannamei</em>) and the model of oxygen management in intensive culture pond. Shrimp in weight of 5 gram were maintained in 20 liter of water in density 6 tails/container. Water quality was measured every 2 hours for 6 hours. Other experiments were done using shrimp in weight of 8, 10, 12 and 15 gram per tail, in density 4 tails/container. The results of study showed that oxygen consumption levels of white shrimp was higher after feeding than before feeding. Oxygen consumption levels of smaller shrimp were higher than that of bigger shrimp. Regression model of oxygen consumption levels before and after feeding were linear. Correlation between oxygen consumption and shrimp weight reached 92.5%. </p> <p>Keywords: white shrimp, <em>Litopenaeus vannamei</em>, oxygen, respiration, intensive pond</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui tingkat konsumsi oksigen udang vaname (<em>Litopenaeus vannamei</em>) dan model pengelolaan oksigen pada tambak intensif. Udang dengan bobot sekitar 5 gram dimasukkan ke dalam wadah tertutup berukuran 20 liter dengan kepadatan 6 ekor/wadah. Kualitas air diukur setiap 2 jam selama 6 jam. Perlakuan tersebut juga dilakukan pada udang dengan kelompok berat 8, 10, 12 dan 15 gram, dengan kepadatan 4 ekor/wadah. Hasil penelitian menunjukkan bahwa tingkat konsumsi oksigen udang vaname sesudah makan relatif lebih tinggi daripada sebelumnya. Tingkat konsumsi oksigen udang ukuran kecil relatif lebih tinggi daripada yang berukuran lebih besar. Model persamaan regresi tingkat konsumsi oksigen pada udang yang tepat sebelum dan sesudah makan adalah linear. Korelasi antara konsumsi oksigen dengan bobot udang mencapai 92,5%. </p> <p>Kata kunci: vaname, <em>Litopenaeus vannamei</em>, oksigen, respirasi, tambak intensif</p>
Effect of L-Ascorbyl-2-Phosphate Magnesium as a Vitamin C Source in Different Doses on Growth of Patin Pangasius Hypophthalmus Fingerlings
Authors: Jusadi D. , Dewantara B.A. , Mokoginta I. 
Published: February 2017

<p>This study was aimed to determine optimum dose of L-Ascorbyl-2-Phosphate Magnesium in the diet of patin, <em>Pangasius hypophthalmus</em> fingerlings. Five isoprotein and isocaloric diets containing different dose of L-Ascorbyl-2-Phosphate Magnesium, i.e. 0, 25, 75 and 100 mg/kg diet were used in this experiment. Fish with an average size of 5.00±0.11 g were maintained at a density of 15 fish per aquarium. Fish were fed three times a day <em>at satiation</em>, for 40 days. The results of study shows that vitamin C content in fish body increased as the vitamin C level of the diet increased. Similar pattern to vitamin C content was also found in protein retention, lipid retention, daily growth rate, and feed efficiency (<em>p</em>< 0.05). Thus, it can be concluded that the most optimum dose of L-Ascorbyl-2-Phosphate Magnesium as vitamin C source for patin fingerlings in this experiment was 100 mg Vit C/kg diet. Higher dose of L-Ascorbyl-2-Phosphate Magnesium than 100 mg/kg diet however need to be further verified.</p> <p>Keywords: vitamin C, patin, <em>Pangasius hypophthalmus</em>.</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini bertujuan untuk menentukan kadar optimum L-Ascorbyl-2-Phosphate Magnesium dalam pakan ikan patin, <em>Pangasius hypophthalmus</em> ukuran sejari. Lima macam pakan isoprotein dan isokalori mempunyai kandungan L-Ascorbyl-2-Phosphate Magnesium berbeda, berturut-turut 0, 25, 50, 75 dan 100 mg/kg pakan telah digunakan dalam penelitian ini. Benih berukuran rata-rata 5,00±0,11 g dipelihara dalam akuarium dengan kepadatan 15 ekor per akuarium. Ikan diberi pakan tiga kali sehari, secara <em>at satiation</em>, selama 40 hari. Hasil penelitian menunjukkan bahwa kadar vitamin C tubuh meningkat sejalan dengan kadar vitamin C pakan. Demikian pula retensi protein, retensi lemak laju pertumbuhan harian, efisiensi pakan mengikuti pola yang sama seperti kandungan vitamin C tubuh (p<0,05). Dengan demikian dapat disimpulkan bahwa kadar vitamin C L-Ascorbyl-2-Phosphate Magnesium terbaik dalam pakan ikan patin adalah 100 mg/kg pakan, namun perlu diteliti lebih lanjut kadar L-Ascorbyl-2 Phosphate Magnesium yang lebih tinggi dari 100 mg/kg pakan.</p> <p>Kata kunci: vitamin C, ikan patin, <em>Pangasius hypophthalmus</em>.</p>
Effect of Feeding Diet Containing Different Dose of Vitamin E with 1:2 of n-3/n-6 Fatty Acid Content on Reproductive Performance of Female Zebrafish Danio rerio
Authors: Utomo N.B.P , Nurjanah N. , Setiawati Mia 
Published: February 2017

<p>The role of essential fatty acids as a precursor of prostaglandin and as a component of cell membrane in the form of phospholipids, can affect cell fluidity. Vitamin E functions as fatty acid antioxidant in the body and affects egg quality. Vitamin E and essential fatty acids (<em>n</em>-3 and <em>n</em>-6) are required at the same time in a proper ratio to induce fish gonad maturation. Zebra fish <em>Danio rerio</em> broodstock in 29 days pre-saline were fed on diet containing 4 different vitamin E concentration; i.e. 325, 375, 425 and 475 mg/kg diet with constant n-3 and n-6 fatty acid doses of 1% and 2%, respectively, for 6 weeks. Reproduction performance in terms of fecundity was improved (p<0.05), whilst gonado somatic index, gonado somatic index saline, fertilization rate, hatching rate, survival rate, yolk absorption rate and daily growth rate were insignificantly different.</p> <p>Keywords: vitamin E, fatty acid, reproduction performance, <em>Danio rerio</em></p> <h1> </h1> <h1>ABSTRAK</h1> <p>Asam lemak esensial berperan sebagai prekursor prostaglandin dan sebagai fosfolipid pada membran sel yang dapat mempengaruhi fluiditasnya. Sedangkan vitamin E berperan sebagai antioksidan asam lemak dalam tubuh sehingga berpengaruh terhadap kualitas telur yang dihasilkan. Vitamin E dan asam lemak essensial (n-3 dan n-6) dibutuhkan secara bersamaan dengan kombinasi perbandingan yang tepat untuk pematangan gonad ikan. Induk ikan yang digunakan dalam penelitian ini adalah ikan zebra<em> Danio rerio</em> yang berumur 29 hari pra salin dan dipelihara selama 6 minggu dengan pakan mengandung kadar vitamin E yang berbeda yaitu 325, 375, 425, dan 475 mg/kg pakan pada kadar asam lemak n-3 (1%) dan n-6 (2 %) tetap. Penampilan reproduksi pra salin ikan tersebut hanya berpengaruh nyata terhadap fekunditas namun tidak berpengaruh nyata terhadap gonado somatik indek, gonado somatik indek salin, derajat pembuahan telur, derajat tetas telur, kelangsungan hidup larva, laju penyerapan kuning telur dan laju pertumbuhan harian.</p> <p>Kata kunci: Vitamin E, asam lemak, penampilan reproduksi, <em>Danio rerio</em></p>
Development of Digestive Enzyme of Patin Pangasius hypohthalmus Larvae
Authors: Effendi I. , Augustine D. , Widanarni . 
Published: February 2017

<p>Culture of patin <em>Pangasius hypophthalmus </em>especially larval rearing very depends on the supply of natural food as energy source. <em>Artemia</em> is the main natural food for fish larvae as a starter food, but its price is high. To reduce production cost, farmers tend to reduce the feeding frequency and shorten the <em>Artemia </em>feeding period. Altering feeding regime however may reduce fry quality. This relate to the availability of digestive enzymes. The objective of this study was to examine digestive enzymes activity in patin larvae fed with different feeding regime. By shorten feeding period with <em>Artemia</em> to 2-4 days and <em>Tubifex</em>,substitution, the enzymes activity of protease, lipase and amylase were revealed similar pattern The enzymes activity tends to increase and reach the peak at day 7 , and decrease later on until day 15 after hatching. Survival rate of fish were varied for each treatment, and the highest survival rate was obtained when larvae were fed by <em>Artemia </em>for 8 days. Blood worm were not fully digested by patin larvae at early stage.</p> <p>Keywords: enzyme, digestion, patin, <em>Pangasius hypophthalmus</em></p> <p> </p> <p>ABSTRAK</p> <p>Proses budidaya ikan patin, <em>Pangasius hypophthalmus</em> terutama pembenihan sangat tergantung oleh ketersediaan pakan alami sebagai sumber energinya. <em>Artemia</em> merupakan pakan alami yang banyak diberikan pada saat larva ikan mulai makan, namun harganya relatif tinggi. Untuk menekan biaya produksi, petani ikan patin cenderung mengurangi frekuensi pemberian <em>Artemia </em>dan mempersingkat waktu pemberiannya. Penggeseran jadwal ini diduga mengakibatkan penurunan kualitas benih ikan patin yang dihasilkan yang berhubungan dengan kesiapan enzim pencernaannya. Penelitian ini dilakukan untuk mengetahui aktivitas enzim pada larva ikan patin dengan jadwal pemberian pakan yang berbeda. Dengan memotong waktu pemberian <em>Artemia</em> 2 - 4 hari dan disubstitusi dengan <em>Tubifex</em>, aktifitas enzim protease, lipase dan amilase pada larva ikan patin, memiliki pola yang sama. Aktifitas enzim cenderung meningkat dan mencapai puncak pada umur 7 hari, selanjutnya terus menurun sampai larva berumur 15 hari. Kelangsungan hidup ikan selama penelitian berbeda-beda untuk setiap perlakuan dengan nilai tertinggi dicapai oleh larva yang diberi pakan <em>Artemia</em> sampai berumur 8 hari. Larva ikan patin belum siap sepenuhnya untuk menerima pakan berupa cacing sutera sejak stadia awalnya.</p> <p>Kata kunci: Enzim, Pencernaan, Patin, <em>Pangasius hypophthalmus</em></p>