bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dimethyl celecoxib sensitizes gastric cancer cells to ABT‐737 via AIF nuclear translocation
Authors: Zhang Bo , Yan Youyou , Li Yangling , Zhang Dan , Zeng Jianmei , Wang Linling , Wang Mimi , Lin Nengming 
Published: July 2016

AbstractGastric cancer is the fourth most common cancer in the world. The clinical applications of both chemotherapy and targeted drugs are limited because of the complexity of gastric cancer. In this study, sulforhodamine B, colony formation assay, 4',6‐diamidino‐2‐phenylindole (DAPI) stain, flow cytometry were used to determine the in vitro cytotoxicity, apoptosis and mitochondrial membrane potential of gastric cancer AGS and HGC‐27 cells before and after treatment. Real‐time PCR and Western blot were used to analyse the mRNA transcription and protein expression respectively. Confocal microscopy was used to determine the localization of target protein within the cells. Treatment with the combination of ABT‐737 and 2,5‐dimethyl‐celecoxib (DMC) showed strong synergistic effect in both AGS and HGC‐27 cells. Moreover, DMC would not influence the intracellular prostaglandin E2 (PGE2) level, thus lacking the toxicity profile of celecoxib. Interestingly, given the significant synergistic effect, combination treatment did not affect the protein expression of BH‐3 proteins including Puma, Noxa and Bim. In combination treatment, cell apoptosis was found independent of caspase‐3 activation. The translocation of apoptosis‐inducing factor (AIF) from mitochondrion to nuclear was responsible for the induced apoptosis in the combination treatment. Taken together, this study provided a novel combination treatment regimen for gastric cancer. Furthermore, the existence of caspase‐independent apoptotic pathway induced by treatment of ABT‐737 was not yet seen until combined with DMC, which shed light on an alternative mechanism involved in Bcl‐2 inhibitor‐induced apoptosis.
Morphology and Molecular Phylogeny of Two Poorly Known Species of Protocruzia (Ciliophora: Protocruziida)
Authors: Jiang Jiamei , Huang Jie , Al‐Farraj Saleh A. , Lin Xiaofeng , Hu Xiaozhong 
Published: July 2016

AbstractThe ciliate genus Protocruzia is a highly confused group, which was formerly placed in the class Heterotrichea or Karyorelictea, and is according to the most recent system tentatively assigned to the class Spirotrichea. In the present study, the morphology, ciliary pattern, and molecular phylogeny of two poorly known species, Protocruzia tuzeti Villeneuve‐Brachon, 1940, and Protocruzia granulosa Kahl, 1933, isolated from coastal waters of China, were investigated. Protocruzia tuzeti differs from its congeners mainly in possessing 6 adoral membranelles, 8–11 somatic kineties, and postoral dikinetids. Protocruzia granulosa is characterized by its extremely slender body, three postoral kineties, and 13 or 14 somatic kineties. The morphogenesis of P. granulosa is similar to that of P. tuzeti, especially in the parakinetal mode of stomatogenesis and the reorganization of the parental paroral membrane; however, more than one somatic kinety joins in the formation of the oral primordium in P. granulosa. Phylogenetic analyses based on small subunit ribosomal RNA gene revealed that six Protocruzia species form a fully supported clade that does not belong to any ciliate class; therefore, our data support the establishment of the class Protocruziea Gao et al. (Sci. Rep., 6, 2016, 24874).
Clinicopathological characteristics of serrated polyps as precursors to colorectal cancer: Current status and management
Authors: Okamoto Koichi , Kitamura Shinji , Kimura Tetsuo , Nakagawa Tadahiko , Sogabe Masahiro , Miyamoto Hiroshi , Muguruma Naoki , Takayama Tetsuji 
Published: July 2016

AbstractSerrated polyps have long been thought to lack malignant potential in the human colorectum. However, identification of the serrated pathway to colorectal cancer based on molecular biology has improved our understanding of the pathogenesis of colorectal cancers. Accordingly, serrated polyps such as traditional serrated adenoma and sessile serrated adenoma/polyps (SSA/P) are now considered to be precursor lesions of the serrated pathway. Recently, serrated polyps were classified into three subtypes, consisting of hyperplastic polyp, SSA/P, and traditional serrated adenoma, according to the World Health Organization classification. It has been suggested that SSA/P in the proximal colon are a precursor lesion of pathogenesis of colorectal cancer and are characterized by BRAF mutation and a CpG island methylator phenotype with or without microsatellite instability. However, SSA/P is more challenging to detect by colonoscopy and is likely to account for some interval cancers, particularly in the proximal colon because it presents flat or sessile, isochroous appearance, and occasionally has a mucous cap. Furthermore, the possibility has been raised that pathologists misclassify SSA/P as hyperplastic polyp. It is important for gastroenterologists to recognize the endoscopic features of serrated polyps to facilitate their detection and removal and also to establish postpolypectomy surveillance guidelines. In this review, we discuss the recent classification of serrated polyps; the molecular characteristics of the serrated pathway; appropriate diagnostic methods using endoscopy, including a new image‐enhanced endoscopic technique; and management of these lesions.
Expression of retinoid‐related orphan receptor (ROR)γt on NK22 cells in the peripheral blood and uterine endometrium of women with unexplained recurrent pregnancy loss and unexplained infertility
Authors: Fuchinoue Kohei , Fukui Atsushi , Chiba Hitomi , Kamoi Mai , Funamizu Ayano , Taima Ayako , Fukuhara Rie , Mizunuma Hideki 
Published: July 2016

AbstractAimRecently, NK22 cells, a subset of interleukin (IL)‐22‐producing natural killer (NK) cells, were identified. We have previously reported the higher percentage of NK22 cells in women suffering recurrent pregnancy loss (RPL). Moreover, we have also reported lower expression of NKp46, a kind of natural cytotoxicity receptor (NCR), on NK cells and the changes of NK cell producing cytokines in women who experience RPL. NK22 cells express NCRs, such as NKp44 or NKp46. Retinoid‐related orphan receptor γt (RORγt) is known as a regulator of NK22 cells; however, in NK22 cells of peripheral blood (PB) and the uterine endometrium (UE), the relationship between NCRs and RORγt is unclear. We investigate RORγt expression NK22 cells in the PB and UE of women with unexplained infertility (uI) or unexplained RPL (uRPL).MethodsLymphocytes were extracted from PB and UE, derived from women with uI or uRPL. Expression of RORγt and NCRs in NK cells and NK cell‐produced cytokines were analyzed by flow cytometry.ResultsCD56+/NKp46+/RORγt+ cells were positively correlated with CD56+/IL‐22+ cells in both PB and UE. CD56bright/NKp46bright/RORγt+ cells were significantly higher in uRPL than in uI, and endometrial CD56bright/NKp46bright/RORγt+ cells were positively correlated with PB. In UE, CD56bright/RORγt+ cells were negatively correlated with CD56bright/interferon‐γ+ and CD56bright/tumor necrosis factor‐α+ cells of uRPL.ConclusionRORγt may be associated with NK22 cells in reproduction. Particularly, higher expression of RORγt may be associated with elevated NK22 cells in uRPL.
Which Resources Matter How and Where? A Meta‐Analysis on Firms’ Foreign Establishment Mode Choice
Authors: Klier Hendrik , Schwens Christian , Zapkau Florian B. , Dikova Desislava 
Published: July 2016

ABSTRACTFirm resources play an important role in explaining the foreign establishment mode choice. However, a theoretical framework that simultaneously a) differentiates between heterogeneous types of resources, b) provides a rationale that consistently explains how firms adapt their establishment mode choice to their resource base, and c) reflects on contextual factors that intervene in the consistency of the direct relationships is largely missing. The present paper draws on the resource‐based view (RBV) to develop such a framework. More specifically, we study the effect of knowledge‐based and experience‐based resources on a firm's establishment mode choice and the moderating impact of cultural distance on these relationships. We validate our theoretical framework by a meta‐analysis (integrating 31 studies with 13,559 establishment mode choices) and find that knowledge‐based resources enhance a firm's propensity for greenfield investments, while experience‐based resources more likely lead to acquisitions. Further, cultural distance increases knowledge‐intensive firms’ preference for greenfield investments.
Evaluation of the LiveWell@School Food Initiative Shows Increases in Scratch Cooking and Improvement in Nutritional Content
Authors: Schober Daniel J. , Carpenter Leah , Currie Venita , Yaroch Amy L. 
Published: July 2016

ABSTRACTBACKGROUNDThe purpose of this evaluation was to examine the effects of the LiveWell@School Food Initiative (LW@SFI), a Colorado‐based childhood obesity prevention program that partners with school districts to enable them to serve more scratch cooked foods through culinary training, action planning, and equipment grants.METHODSThis evaluation used a quasi‐experimental design that examined menu cycles prior to entering the LW@SFI and approximately 1 year later. A review of school menus with food service directors from 9 Colorado school districts was conducted.RESULTSData show that districts changed an average of 17.4 entrées and 19.7 side dishes over the course of the year. Changes to serving scratch cooked foods were highest for sauces (an increase of 40.5%). No districts were cooking beans/legumes from scratch during baseline or at follow‐up. Across the 9 districts, 7 observed statistically significant pre‐post reductions in sodium, 4 in fat, 5 in saturated fat, and 3 in calories.CONCLUSIONSWithin a year of implementing the LW@SFI, school districts increased the proportion of fresh, scratch cooked foods they offered and this was associated with some decreases in calories, fat, saturated fat, and sodium, contributing to healthier school food environments.
Phylogenetic position of two endemic Carthamus species in Algeria and their potential as sources of genes for water use efficiency improvement of safflower
Authors: Mihoub Imane , Robert Thierry , Ghashghaie Jaleh , Vilatersana Roser , Lamy Françoise , Benmrid Reda , Lamothe‐Sibold Marlène , Aid Fatiha 
Published: July 2016

AbstractDespite the high economic value of safflower (Carthamus tinctorius), the potential of several wild related species as genetic resources is still poorly studied, in particular with regards to drought tolerance, a priority in safflower improvement. This situation is partially explained by the numerous and still discussed uncertainties in phylogenetic relationships within the safflower species complex. In this study, we focused on two rare Algerian wild species, Carthamus strictus and Carthamus chouletteanus. We aimed at: (i) clarifying their taxonomic status, and determining their phylogenetic relationships with safflower and other Mediterranean species of Carthamus sensu lato (s.l.) (Carthamus sensu stricto (s.s.) and Carduncellus genera) by using nucleotide diversity within the internal transcribed spacer region (ITS) and (ii) assessing the water use efficiency (WUE) of these species by determining their carbon isotope composition. Our results support the existence of two well supported different clusters among the safflower species complex: Carthamus s.s. including Eastern annual species and the vast Carduncellus group constituted by Western perennial species, including the two studied Algerian species. Also, significant differences for carbon isotopic composition between Carthamus s.s. and Carduncellus were revealed. Carduncellus species displayed significantly higher δ13C values (i.e., higher WUE) than Carthamus s.s. species. Carthamus chouletteanus displayed the highest average value of δ13C (highest WUE) among the species studied. Our data encourage for further studies on the possibility of hybridisations between species of the two genera and suggest that Carduncellus should be considered as potentially more interesting genetic resources to improve the WUE of safflower, rather than Carthamus s.s. species.
Baseline HBsAg and HBcrAg titres allow peginterferon‐based ‘precision medicine’ in HBeAg‐negative chronic hepatitis B patients
Authors: Martinot‐Peignoux M. , Lapalus M. , Maylin S. , Boyer N. , Castelnau C. , Giuily N. , Pouteau M. , Moucari R. , Asselah T. , Marcellin P. 
Published: July 2016

SummaryQuantitative hepatitis B core‐related antigen (qHBcrAg) has been proposed as an additional marker to quantitative HBsAg (qHBsAg), for management of chronic hepatitis B. Evaluate baseline combination of qHBsAg and qHBcrAg for identification of patients that could benefit from pegylated interferon‐alpha‐2a (PegIFN)‐based therapy. Sixty‐two HBeAg‐negative patients treated with PegIFN or PegIFN plus tenofovir disoproxil fumarate (PegIFN+TDF). HBsAg and HBcrAg titres were evaluated at baseline. Thirty patients received PegIFN and 32 PegIFN+TDF. SR was 10 of 30 and 17 of 32 in PegIFN and PegIFN+TDF patients, respectively. Cut‐offs determined by maximized Youden's index for identifying patients likely to respond to therapy were as follows: 3.141 log10 IU/mL and 3.450 log10 U/mL for HBsAg and HBcrAg, respectively. At the end of 3 years post‐treatment follow‐up, HBsAg loss was observed in 7 of 30 and 6 of 32 in PegIFN and PegIFN+TDF patients, respectively. The AUC was estimated to be 0.716 (95% CI [0.578, 0.855]) for HBsAg and 0.668 (95% CI [0.524, 0.811]) for HBcrAg (P=.5541). PPVs for AUCs(95%CI) were 0.762(0.590–0.947), 0.714(0.533–1.000) and 0.800(0.611–1.000), and NPVs for AUCs(95%CI) were 0.756(0.660–0.899), 0.718(0.630–0.857) and 0.765(0.675–0.889) for qHBsAg, qHBcrAg and the combination of both markers, respectively. Baseline qHBsAg 3.141 log10 IU/mL and qHBcrAg 3.450 log10U/mL thresholds used separately or in combination allow prediction of response, prior to PegIFN‐based therapy, with a PPV of 80.3% and NPV of 76.5%. Baseline qHBsAg is predictive of HBsAg loss. Both markers could be used, separately or in combination, for PegIFN‐based ‘precision therapy’. Our results emphasize that the combination of PegIFN alpha‐2a plus TDF with 53% of SR might be an alternative to finite therapy.
Effect of Dietary Gamma‐irradiated and Fermented Soybean Meal on the Growth Performance, Body Composition, and Digestive Enzymes Activity of Caspian Brown Trout, Salmo trutta caspius, Juvenile
Authors: Sotoudeh Ebrahim , Amiri Moghaddam Jamshid , Shahhosseini Gholamreza , Aramli Mohammad Sadegh 
Published: July 2016

AbstractThis experiment was conducted to evaluate the feasibility of gamma‐irradiated soybean meal (SBM) as a dietary ingredient on the performance, body composition, and digestive enzyme activity of Caspian brown trout, Salmo trutta caspius, juveniles. Five isonitrogenous and isoenergetic diets were formulated, in which fish meal was replaced by untreated (untreated SBM), gamma‐ray irradiated at 15 (15ISBM) and 30 (30ISBM) kGy, fermented (FSBM), fermented 15 KGy irradiated soybean meal (FISBM), respectively. Juvenile fish (2.1 ± 0.3 g) were fed with the test diets for 6 wk. Final body weight was significantly affected by dietary SBM treatment with highest values in fish fed FISBM and FSBM (P < 0.05). There were no significant differences in hepatosomatic and visceral indexes, condition factor, and survival among experimental groups (P > 0.05). The carcass proximate composition of lipid and protein in Caspian brown trout in FSBM and FISBM groups was higher than that of other groups. Levels of most whole‐body amino acids were not significantly different among Caspian brown trout fed the five diets. Results of this experiment showed that replacing SBM with FSBM and FISBM in the diet significantly increased the activities of proteolytic enzymes activities (P < 0.05). However, no significant differences were observed in both amylase and lipase activities among dietary treatments. Results of this study indicated that the level of gamma radiation used did not work but fermentation of SBM did increase some growth parameters of Caspian brown trout.
Forecasting the Genetic Impacts of Net Pen Failures on Gulf of Mexico Cobia Populations Using Individual‐based Model Simulations
Authors: Darden Tanya L. , Robinson John D. , Strand Allan E. , Denson Michael R. 
Published: July 2016

AbstractOffshore net pen fish farming provides a cost‐efficient means for production of marine finfish, and there is great interest in development of net pen operations in domestic waters. However, there are concerns over the possible genetic and ecological impacts that escaped fish may have on wild populations. We used individual‐based simulations, with parameter values informed by life history and genetic data, to investigate the short‐term (50 yr) impacts of net pen failures on the genetic composition of cobia, Rachycentron canadum, stocks in the Gulf of Mexico. Higher net pen failure rates resulted in greater genetic impacts on the wild population. Additionally, the use of more genetically differentiated source populations led to larger influxes of non‐native alleles and greater temporal genetic change in the population as a result of net pen failure. Our results highlight the importance of considering the appropriate source population for broodstock collection in net pen aquaculture systems and help to provide a general set of best management practices for broodstock selection and maintenance in net pen aquaculture operations. A thorough understanding of the genetic diversity, stock structure, and population demography of target species is important to determine the impact escapees can have on wild populations.
The Pragmatic Encroachment Debate
Authors: Roeber Blake 
Published: July 2016

AbstractDoes knowledge depend in any interesting way on our practical interests? This is the central question in the pragmatic encroachment debate. Pragmatists defend the affirmative answer to this question while purists defend the negative answer. The literature contains two kinds of arguments for pragmatism: principle‐based arguments and case‐based arguments. Principle‐based arguments derive pragmatism from principles that connect knowledge to practical interests. Case‐based arguments rely on intuitions about cases that differ with respect to practical interests. I argue that there are insurmountable problems for both kinds of arguments, and that it is therefore unclear what motivates pragmatism.
Knockdown of strigolactone biosynthesis genes in Populus affects BRANCHED1 expression and shoot architecture
Authors: Muhr Merlin , Prüfer Nele , Paulat Maria , Teichmann Thomas 
Published: July 2016

Summary

Plant architecture is modified by a regulatory system that controls axillary bud outgrowth. Key components in this system are strigolactones (SLs) and BRANCHED1, which inhibit bud outgrowth. Their role has been described in herbaceous model systems, including Arabidopsis, rice and pea. However, a role in woody perennial species, including the model tree poplar, has not been unequivocally proven.

In this study, we tested a role for SLs in Populus × canescens by treatment with the synthetic SL GR24. We generated MORE AXILLARY BRANCHING4 (MAX4) knockdown lines to study the architectural phenotype of poplar SL biosynthesis mutants and the expression of SL‐regulated genes.

We show that GR24 is perceived by the model tree poplar. MAX4 knockdown lines exhibit typical SL deficiency symptoms. The observed changes in branching pattern, internode length and plant height can be rescued by grafting. We identified putative poplar BRANCHED1 and BRANCHED2 genes and provide evidence for a regulation of BRANCHED1 by SLs.

Our results suggest a conservation of major regulatory mechanisms in bud outgrowth control in the model tree poplar. This may facilitate further research, pinpointing the role of SLs and BRANCHED1 in the complex regulation of bud outgrowth in trees.
Does leaf shedding protect stems from cavitation during seasonal droughts? A test of the hydraulic fuse hypothesis
Authors: Wolfe Brett T. , Sperry John S. , Kursar Thomas A. 
Published: July 2016

Summary

During droughts, leaves are predicted to act as ‘hydraulic fuses’ by shedding when plants reach critically low water potential (Ψplant), thereby slowing water loss, stabilizing Ψplant and protecting against cavitation‐induced loss of stem hydraulic conductivity (Ks).

We tested these predictions among trees in seasonally dry tropical forests, where leaf shedding is common, yet variable, among species. We tracked leaf phenology, Ψplant and Ks in saplings of six tree species distributed across two forests.

Species differed in their timing and extent of leaf shedding, yet converged in shedding leaves as they approached the Ψplant value associated with a 50% loss of Ks and at which their model‐estimated maximum sustainable transpiration rate approached zero. However, after shedding all leaves, the Ψplant value of one species, Genipa americana, continued to decline, indicating that water loss continued after leaf shedding. Ks was highly variable among saplings within species and seasons, suggesting a minimal influence of seasonal drought on Ks.

Hydraulic limits appear to drive diverse patterns of leaf shedding among tropical trees, supporting the hydraulic fuse hypothesis. However, leaf shedding is not universally effective at stabilizing Ψplant, suggesting that the main function of drought deciduousness may vary among species.
Evaluation of a Staff Training Programme using Positive Psychology coaching with film and theatre elements in care homes: views and attitudes of residents, staff and relatives
Authors: Guzmán Azucena , Wenborn Jennifer , Ledgerd Ritchard , Orrell Martin 
Published: July 2016

BackgroundThere is a recognised need to improve staff training in care homes. The aim of this study was to conduct a qualitative evaluation of the Ladder to the Moon Culture Change Studio Engagement Programme (CCSEP), a staff training programme aimed at enhancing staff–resident communication.MethodFocus groups were conducted with residents able to provide consent; staff and relatives and managers were interviewed in two care homes. A theoretical framework was developed to interpret the impact of CCSEP using Framework Analysis.ResultsResidents noted that the programme appeared to result in staff interacting more with them, as well as enjoying working together as a team. Staff reported an improved sense of teamwork, developing more positive attitudes towards residents, as well as their concerns about using theatrical techniques in the care setting. Relatives identified care home organisational aspects as being barriers to implementation, and some regarded CCSEP simply as ‘entertainment’ rather than ‘creative care’.DiscussionThis study provides an insight into the potential of this staff training programme to improve staff–resident interactions. However, participants' varying views of CCSEP highlight the need to brief staff, residents and relatives before implementation so as to enable full understanding of the aim.
Adorno's Negative Dialectics
Authors: Sherman David 
Published: July 2016

AbstractThe concept of negative dialectics constitutes the philosophical core of Adorno's wide‐ranging thought. It reflects his attempt both to consider the status of dialectics in the face of a history that has failed to actualize its prognostications and to rework dialectics to make it adequate to his own time. Among the themes considered are Adorno's critique of conceptuality in the German idealist tradition, his critique of enlightenment reason and its relationship to capitalist society, his qualified rejection of universal history, his concern with revitalizing experience, and his concept of the constellation, which he offers as an alternative to existing epistemological paradigms.