bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
Authors: Ma Baowei , Hu Tianyuan , Li Pei , Yuan Qingjun , Lin Zhaoshou , Tu Yuhe , Li Jia , Zhang Xianan , Wu Xiaoyi , Wang Xiujuan , Huang Luqi , Gao Wei 
Published: September 2017

AbstractTripterygium wilfordii (Celastraceae) is a traditional Chinese medicine; and the dried root and rhizome constitute the main officinal parts. Tripterygium wilfordii has been identified as a potential candidate for the treatment of systemic lupus erythematosus, rheumatoid arthritis, nephritis, asthma, leprosy, and cancer. The phylogenetic relationships within the Tripterygium genus are ambiguous; thus, our aim is to clarify the relationships within this genus using phylogeographic and phylogenetic analyses. Here, we first sequenced three plastid DNA regions (i.e., psbA‐trnH, rpl32‐trnL, and trnL‐trnF) and found that Tripterygium hypoglaucum and T. wilfordii were clustered together based on the strength of the topology in the phylogenetic analysis: T. hypoglaucum is polyphyletic, and T. wilfordii is paraphyletic. A spatial analysis of molecular variance showed that the best group value is 4, and the groups were almost consistent with the topology of in the phylogenetic analysis. The Mantel analyses of Tripterygium using IBD web showed statistically significant relationships between genetic and geographical distance distributions (r = .3479, p < .0001). The molecular dating using Fossil calibration indicated that the divergence in Tripterygium was approximately 8.13 Ma. Furthermore, we also analyzed four DNA regions (i.e., ITS2, psbA‐trnH, matK, and rbcL) that were obtained from the NCBI nucleotide database; these results showed that T. wilfordii and T. hypoglaucum clustered together, while Tripterygium regelii represented a separate cluster. Tripterygium hypoglaucum and T. wilfordii were never distinct lineages, and the species circumscriptions are artificial. We propose that T. wilfordii and T. hypoglaucum are conspecific, while T. regelii likely constitutes a separate species.
Predicting individual plant performance in grasslands
Authors: Herz Katharina , Dietz Sophie , Haider Sylvia , Jandt Ute , Scheel Dierk , Bruelheide Helge 
Published: September 2017

AbstractPlant functional traits are widely used to predict community productivity. However, they are rarely used to predict individual plant performance in grasslands. To assess the relative importance of traits compared to environment, we planted seedlings of 20 common grassland species as phytometers into existing grassland communities varying in land‐use intensity. After 1 year, we dug out the plants and assessed root, leaf, and aboveground biomass, to measure plant performance. Furthermore, we determined the functional traits of the phytometers and of all plants growing in their local neighborhood. Neighborhood impacts were analyzed by calculating community‐weighted means (CWM) and functional diversity (FD) of every measured trait. We used model selection to identify the most important predictors of individual plant performance, which included phytometer traits, environmental conditions (climate, soil conditions, and land‐use intensity), as well as CWM and FD of the local neighborhood. Using variance partitioning, we found that most variation in individual plant performance was explained by the traits of the individual phytometer plant, ranging between 19.30% and 44.73% for leaf and aboveground dry mass, respectively. Similarly, in a linear mixed effects model across all species, performance was best predicted by phytometer traits. Among all environmental variables, only including land‐use intensity improved model quality. The models were also improved by functional characteristics of the local neighborhood, such as CWM of leaf dry matter content, root calcium concentration, and root mass per volume as well as FD of leaf potassium and root magnesium concentration and shoot dry matter content. However, their relative effect sizes were much lower than those of the phytometer traits. Our study clearly showed that under realistic field conditions, the performance of an individual plant can be predicted satisfyingly by its functional traits, presumably because traits also capture most of environmental and neighborhood conditions.
Emerging roles of syndecan 2 in epithelial and mesenchymal cancer progression
Authors: Mytilinaiou Maria , Nikitovic Dragana , Berdiaki Aikaterini , Kostouras Antonis , Papoutsidakis Antonis , Tsatsakis Aristidis M. , Tzanakakis George N. 
Published: September 2017

AbstractSyndecan 2 (SDC2) belongs to a four‐member family of evolutionary conserved small type I transmembrane proteoglycans consisting of a protein core to which glycosaminoglycan chains are covalently attached. SDC2 is a cell surface heparan sulfate proteoglycan, which is increasingly drawing attention for its distinct characteristics and its participation in numerous cell functions, including those related to carcinogenesis. Increasing evidence suggests that the role of SDC2 in cancer pathogenesis is dependent on cancer tissue origin rendering its use as a biomarker/therapeutic target feasible. This mini review discusses the mechanisms, through which SDC2, in a distinct manner, modulates complex signalling networks to affect cancer progression. © 2017 IUBMB Life, 69(11):824–833, 2017
Multimodal pore formation in calcium phosphate cements
Authors: Lodoso‐Torrecilla Irene , van Gestel Nicole A. P. , Diaz‐Gomez Luis , Grosfeld Eline‐Claire , Laperre Kjell , Wolke Joop G. C. , Smith Brandon T. , Arts Jacobus J. , Mikos Antonios G. , Jansen John A. , Hofmann Sandra , van den Beucken Jeroen J. J. P. 
Published: September 2017

AbstractCalcium phosphate cements (CPCs) are commonly used as bone substitute materials. However, their slow degradation rate and lack of macroporosity hinders new bone formation. Poly(dl‐lactic‐co‐glycolic acid) (PLGA) incorporation is of great interest as, upon degradation, produces acidic by‐products that enhance CPC degradation. Yet, new bone formation is delayed until PLGA degradation occurs a few weeks after implantation. Therefore, the aim of this study was to accelerate the early stage pore formation within CPCs in vitro. With that purpose, we incorporated the water‐soluble porogen sucrose at different weight percentages (10 or 20 wt %) to CPC and CPC/PLGA composites. The results revealed that incorporation of sucrose porogens increased mass loss within the first week of in vitro degradation in groups containing sucrose compared to control groups. After week 1, a further mass loss was observed related to PLGA and CPC degradation. Macroporosity analysis confirmed that macroporosity formation is influenced by the dissolution of sucrose at an early stage and by the degradation of PLGA and CPC at a later stage. We concluded that the combination of sucrose and PLGA porogens in CPC is a promising approach to promote early stage bone tissue ingrowth and complete replacement of CPC through multimodal pore formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 500–509, 2018.
ASFA Category IV becomes Category I: Idiopathic thrombotic thrombocytopenic purpura in a patient with presumed gemcitabine‐induced thrombotic microangiopathy
Authors: Bittar Peter G. , Nickolich Myles S. , Onwuemene Oluwatoyosi A. 
Published: September 2017

AbstractIn the implementation of American Society for Apheresis national guidelines, the decision for therapeutic plasma exchange may be confounded by a clinical presentation that fits both a Category I and IV designation. We report the case of a 45‐year‐old female who presented with concern for a Category IV disorder, gemcitabine‐induced thrombotic microangiopathy, and was ultimately diagnosed with a Category I disorder, idiopathic thrombotic thrombocytopenic purpura. This case highlights the importance of ruling out idiopathic TTP by a thorough evaluation for ADAMTS13 activity and inhibitor, even when an alternate thrombotic microangiopathy diagnosis may be likely.
Effects of placebo‐controlled insulin‐sensitizing drugs on hormonal parameters in polycystic ovary syndrome patients: A network meta‐analysis
Authors: Huang Rong , Zhao Peng‐Fei , Xu Jian‐Hua , Liu Dong‐Dong , Luo Fu‐Dong , Dai Yong‐Hui 
Published: September 2017

AbstractThis network meta‐analysis was conducted to compare effects of different placebo‐controlled insulin‐sensitizing drugs, including metformin, pioglitazone, rosiglitazone, and troglitazone on hormonal parameters in polycystic ovary syndrome (PCOS) patients. We searched PubMed, EMBASE, and Cochrane Library databases from their inception to July 2017. Randomized controlled trials (RCTs) met our inclusion criteria were included. We combined direct and indirect evidences to evaluate weighted mean difference (WMD) value and draw surface under the cumulative ranking curve (SUCRA). Totally 28 eligible RCTs were enrolled. The network meta‐analysis results indicated that: Compared with placebo, patients treated with pioglitazone had relatively higher sex‐hormone‐binding globulin (SHBG) (nmol/L) level (WMD = 6.65, 95%CI = 0.57‐12.98), patients treated with metformin had comparatively lower total testosterone (TT) (ng/mL) level (WMD = −0.20, 95%CI = −0.39 to −0.02); Compared with rosiglitazone, patients treated with metformin had relatively higher estradiol (E2) (pg/mL) level (WMD = 47.91, 95%CI = 11.44‐85.55). However, there were no statistical significance among the placebo‐controlled insulin‐sensitizing drugs in follicle stimulating hormone (FSH) (IU/L), luteinizing hormone (LH) (IU/L), dehydroepiandrostrone‐sulphate (DHEAS) (µg/dL), free testosterone (FT) (pg/mL) and androstenedione (ng/mL). The results of cluster analysis showed that rosiglitazone may be the best drug for PCOS patients regarding to DHEAS, TT, FSH, and LH, metformin may be the best drug for PCOS patients as for E2, FT, and androstenedione. Rosiglitazone had the best effect on PCOS patients in terms of DHEAS, TT, FSH, and LH, metformin had the best effect on PCOS patients for E2, FT, and androstenedione.
Collagen, agarose, alginate, and Matrigel hydrogels as cell substrates for culture of chondrocytes in vitro: A comparative study
Authors: Miao Zhikang , Lu Zhenhui , Wu Huayu , Liu Hui , Li Muyan , Lei Danqing , Zheng Li , Zhao Jinmin 
Published: September 2017

AbstractAutologous chondrocyte implantation (ACI) has emerged as a new approach to cartilage repair through the use of harvested chondrocytes. But the expansion of the chondrocytes from the donor tissue in vitro is restricted by limited cell numbers and dedifferentiation of chondrocytes. In this study, we used four types of hydrogels including agarose, alginate, Matrigel, and collagen type I hydrogels to serve as cell substrates and investigated the effect on proliferation and phenotype maintenance of chondrocytes. As a substrate for monolayer culture, collagen facilitated cell expansion and effectively suppressed the dedifferentiation of chondrocytes, as evidenced by fluorescein diacetate/propidium iodide (FDA/PI), hematoxylin‐eosin staining (HE), Safranin O, immunofluorescenceassay, biochemistry analysis, and quantitative real‐time polymerase chain reaction (qRT‐PCR). Compared with that in agarose gels, alginate, and Matrigel, collagen accelerated cell proliferation and enhanced the expression of cartilage specific genes such as ACAN, SOX9, and COLII more markedly. Furthermore, significantly lower expression of COL I (an indicator of dedifferentiation) and COL X (the chondrocyte hypertrophy marker) was present in collagen group than in other groups. This indicated that collagen substrate can better support chondrocyte growth and maintain cell phenotype, due to that it might serve as a cartilage‐like ECM to provide adhesive site for chondrocytes. In summary, collagen hydrogel is a promising cell substrate for chondrocytes culture for ACI.
Inducing indel mutation in the SOX6 gene by zinc finger nuclease for gamma reactivation: An approach towards gene therapy of beta thalassemia
Authors: Modares Sadeghi Mehran , Shariati Laleh , Hejazi Zahra , Shahbazi Mansoureh , Tabatabaiefar Mohammad Amin , Khanahmad Hossein 
Published: September 2017

Abstractβ‐thalassemia is a common autosomal recessive disorder characterized by a deficiency in the synthesis of β‐chains. Evidences show that increased HbF levels improve the symptoms in patients with β‐thalassemia or sickle cell anemia. In this study, ZFN technology was applied to induce a mutation in the binding domain region of SOX6 to reactivate γ‐globin expression. The sequences coding for ZFP arrays were designed and sub cloned in TDH plus as a transfer vector. The ZFN expression was confirmed using Western blot analysis. In the next step, using the site‐directed mutagenesis strategy through the overlap PCR, a missense mutation (D64V) was induced in the catalytic domain of the integrase gene in the packaging plasmid and verified using DNA sequencing. Then, the integrase minus lentivirus containing ZFN cassette was packaged. Transduction of K562 cells with this virus was performed. Mutation detection assay was performed. The indel percentage of the cells transducted with lenti virus containing ZFN was 31%. After 5 days of erythroid differentiation with 15 μg/mL cisplatin, the levels of γ‐globin mRNA were sixfold in the cells treated with ZFN compared to untreated cells. In the meantime, the measurement of HbF expression levels was carried out using hemoglobin electrophoresis and showed the same results. Integrase minus lentivirus can provide a useful tool for efficient transient gene expression and helps avoid disadvantages of gene targeting using the native virus. The ZFN strategy applied here to induce indel on SOX6 gene in adult erythroid progenitors may provide a method to activate fetal hemoglobin expression in individuals with β‐thalassemia.
Retracted: In vivo and in vitro effects of microRNA‐124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway
Authors: Xiao Hai‐Juan , Ji Qing , Yang Lin , Li Ren‐Ting , Zhang Cheng , Hou Jun‐Ming 
Published: September 2017

AbstractIn this study, we aim to determine the function of miR‐124 on gastric cancer (GC) cells and the underlying mechanism that involves jaddeg1 (JAG1) and the Notch signaling pathway. GC tissues and adjacent tissues from 100 patients suffering from GC were selected. GC SGC‐7901 and AGS cells were selected and grouped into control, mimic‐NC, miR‐124 mimic, inhibitor‐NC, miR‐124 inhibitor, and miR‐124 inhibitor + si‐JAG1 groups. RT‐qPCR and a Western blotting assay were conducted to detect the expression of miR‐124, JAG1, and Notch signaling pathway‐related proteins (NICD, HES1, and HES5). MTS, wound‐healing, transwell assay and flow cytometry were performed to detect cell proliferation, migration, invasion, cell cycle distribution, and apoptosis, respectively. Compared with adjacent tissues, a lower miR‐124 expression and higher JAG1 expression were found in GC tissues. JAG1 is a direct target gene of miR‐124. Compared with the control group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration, and proliferation in the miR‐124 mimic group were decreased, while the apoptosis rate was increased and cells were arrested at the G0/G1 phase. Compared with the miR‐124 inhibitor group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration, and proliferation in the miR‐124 inhibitor + si‐JAG1 group were decreased, while the apoptosis rate and cell ratio at the G0/G1 phase were increased. The demonstration that miR‐124 inhibits GC cell growth supports the concept that miR‐124 functions as a tumor suppressor by a mechanism that involves translational repression of the JAG1 and the inhibition of Notch signaling pathway.
Retracted: SHH gene silencing suppresses epithelial‐mesenchymal transition, proliferation, invasion, and migration of cervical cancer cells by repressing the hedgehog signaling pathway
Authors: Zhang Feng , Ren Chen‐Chen , Liu Ling , Chen Yan‐Nan , Yang Li , Zhang Xiao‐An , Wang Xiao‐Ming , Yu Feng‐Jing 
Published: September 2017

AbstractThe above article, published online on 27 October 2017 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor in Chief, Gary Stein and Wiley Periodicals, Inc. The retraction has been agreed due to determination by the authors that the data relating to infiltration and metastasis presented in this publication is incomplete and there is need for further study and analysis.ReferenceZhang F, Ren C‐C, Liu L, et al. SHH gene silencing suppresses epithelial‐mesenchymal transition, proliferation, invasion, and migration of cervical cancer cells by repressing the hedgehog signaling pathway. J Cell Biochem. 2018;119:3829–3842. https://doi.org/10.1002/jcb.26414.
Process design of carbon dioxide and ethane separation using ionic liquid by extractive distillation
Authors: Zhu Zhaoyou , Hu Jiajing , Geng Xueli , Qin Bin , Ma Kang , Wang Yinglong , Gao Jun 
Published: September 2017

AbstractBACKGROUNDCarbon dioxide and ethane easily form a binary azeotrope under liquefied conditions. An extractive distillation process for the separation of carbon dioxide and ethane using the ionic liquids [bmim][Tf2N], [emim][Tf2N] and [emim][EtSO4] as solvents was explored. The thermodynamic and physical property parameters of each ionic liquid and their thermo‐physical properties that correlated with the experimental data were used to create the ionic liquid components and conduct the process simulation.RESULTBased on the analysis of its physical properties and relative volatilities, [emim][Tf2N] is the best option as a solvent for extractive distillation separation compared with other ionic liquids. Based on the sensitivity analysis optimization procedure, the minimum total annual cost, carbon dioxide emissions and thermodynamic efficiency were calculated. Compared with traditional extraction distillation, the total annual cost and carbon dioxide emissions of the process using [emim][Tf2N] as a solvent decreased by 62% and 31%, respectively. The thermodynamic efficiency (η) of the electricity generated from hydropower increase by 0.7%.CONCLUSIONThe results show that an ionic liquid extractive distillation process using an ionic liquid as the entrainer performs better than a process using traditional organic solvents in terms of economics. Due to the non‐volatility of ionic liquids, a flash tank was used instead of a solvent recovery column to efficiently realize solvent recovery. © 2017 Society of Chemical Industry
Changes in pulmonary artery systolic pressure correlate with radiographic severity and peripheral oxygenation in adults with community‐acquired pneumonia
Authors: Sreter Katherina Bernadette , Budimir Ivan , Golub Andrija , Dorosulić Zdravko , Sabol Pušić Mateja , Boban Marko 
Published: September 2017

AbstractPurposeThe aim of this prospective observational study was to evaluate the relationship between changes in pulmonary artery systolic pressure (ΔPASP) and both severity of community‐acquired pneumonia (CAP) and changes in peripheral blood oxygen partial pressure (PaO2).Materials and MethodsSeventy‐five consecutive adult patients hospitalized for treatment of CAP were recruited in this single‐center cohort study. Doppler echocardiographic measurement of PASP was performed by 2 staff cardiologists. Follow‐up assessment was performed within 2 to 4 weeks of ending antibiotic treatment at radiographic resolution of CAP. Fifteen patients were excluded during follow‐up due to confirmation of chronic obstructive pulmonary disease.ResultsPneumonia was unilateral in 40 (66.7%) and bilateral in 20 (33.3%) patients. Radiographic extent of pneumonia involved 2 pulmonary segments in 31 patients (51.7%), 3 to 5 pulmonary segments in 25 (41.7%), and 6 pulmonary segments in 4 patients (6.6%). ΔPASP between hospital admission and follow‐up correlated with the number of pulmonary segments involved (Rho = 0.953; P < .001) and PaO2 (Rho = −0.667; P < .001). The maximum PASP was greater during pneumonia than after resolution (34.82 ± 3.96 vs. 22.67 ± 4.04, P < .001).ConclusionsChanges in PASP strongly correlated with radiological severity of CAP and PaO2. During pneumonia, PASP appeared increased without significant change in left ventricular filling pressures. This suggests that disease‐related changes in lung tissue caused by pneumonia may easily and reproducibly be assessed using conventional noninvasive bedside diagnostics such as echocardiography and arterial blood gas analysis.
Mediastinal ectopic thyroid diagnosed by endobronchial ultrasound‐guided transbronchial needle aspiration: Report of three cases
Authors: Ozturk Ayperi , Cicek Tugba , Aktas Zafer , Demirag Funda , Yilmaz Aydin 
Published: September 2017

AbstractAn ectopic thyroid gland results from the abnormal migration of the thyroid in the course of its development. Primary ectopic mediastinal thyroid is very rare and occurs in less than 1% of all goiters that can be surgically excised. Ectopic thyroid tissue has a characteristic sonographic appearance as smooth‐bordered, homogeneous, hypoechoic tissue with fine specular echoes. We report 3 cases of mediastinal ectopic thyroid diagnosed by endobronchial ultrasound‐guided transbrochial needle aspiration biopsy.
Polymorphisms in homologous recombination repair genes and the risk and survival of breast cancer
Authors: Liao Yu‐Huang , Ren Jun‐Ting , Zhang Wei , Zhang Zheng‐Zheng , Lin Ying , Su Feng‐Xi , Jia Wei‐Hua , Tang Lu‐Ying , Ren Ze‐Fang 
Published: September 2017

AbstractBackgroundImmunoglobulin (Ig)A antibody of Epstein–Barr virus (EBV) was found to associate with breast cancer (BC), whereas IgA positivity was related to a series of genetic markers in the genes of homologous recombination repair system (HRRs). We assessed the associations of the polymorphisms in HRR genes with the risk and survival of BC.MethodsA case–control study was conducted with 1551 bc cases and 1605 age‐matched healthy controls between October 2008 and March 2012 in the Guangzhou Breast Cancer Study (GZBCS), China, and the case population were followed up until 31 January 2016. Five single nucleotide polymorphisms of candidate genes in HRR system were genotyped. Odds ratios (ORs) and hazards ratios (HRs) were calculated using multivariate logistic regression and Cox proportional hazards regression to estimate the risk and prognostic effect, respectively.ResultsRFC1 rs6829064 (AA) was associated with an increased BC risk [OR = 1.35; 95% confidence interval (CI) = 1.06–1.73] compared to the wild genotype (GG). NRM rs1075496 (GT/TT versus GG) was associated with a worse progression‐free survival (PFS) and the HR was 1.34 (95% CI = 1.01–1.78), particularly among advanced patients. LIG3 rs1052536 (CT/TT versus CC) was associated with a better PFS and the HR was 0.70 (95% CI = 0.53–0.93). However, RAD54L rs1710286 and RPA1 rs11078676 were not observed to be associated with either the risk or survival of BC.ConclusionsThe findings of the present study suggest that the polymorphisms in HRR genes were associated with BC risk (RFC1 rs6829064) and prognosis (NRM rs1075496 and LIG3 rs1052536), whereas RAD54L rs1710286 and RPA1 rs11078676 had null associations with BC.