bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin Erythema, Pigmentation and Hydration Kinetics after Ultraviolet Radiation‐induced Photodamage in Southern Chinese Women
Authors: Wan Miaojian , Hu Rong , Xie Xiaoyuan , Gong Zijian , Yi Jinling , Chen Haiyan , Xie Lin , Guan Xiaomin , Guan Lei , Lai Wei 
Published: March 2017

AbstractAlthough there have been some studies about changes of skin erythema and pigmentation following ultraviolet radiation in other races, the relevant data in Chinese have never been achieved. Thus, we evaluated the long‐time course of skin erythema, pigmentation and hydration changes after different doses of solar‐simulated ultraviolet (SSUV) irradiation in 26 Chinese women for 168 days. The erythema index increased abruptly and peaked during 3 days of SSUV exposure, then slowly returned to the baseline level starting at day 7 and completely recovered during 168‐day course of this study only in one minimal erythema doses (MED) SSUV irradiation. The melanin index started to slowly increase at day 3 of SSUV exposure, peaking at day 14 and gradually returned to the baseline level thereafter, but did not return to the baseline level during 168‐day course in all doses. Skin hydration slowly declined at day 3 of exposure, hitting the lowest point at day 7, then slowly recovered starting at day 14 and completely returned to the baseline level at day 28 only in 1.5MED. These results will serve as baseline data on Chinese skin and provide useful references for the treatment of serious skin photodamage in Chinese.
Working memory capacity and task goals modulate error‐related ERPs
Authors: Coleman James R. , Watson Jason M. , Strayer David L. 
Published: March 2017

AbstractThe present study investigated individual differences in information processing following errant behavior. Participants were initially classified as high or as low working memory capacity using the Operation Span Task. In a subsequent session, they then performed a high congruency version of the flanker task under both speed and accuracy stress. We recorded ERPs and behavioral measures of accuracy and response time in the flanker task with a primary focus on processing following an error. The error‐related negativity was larger for the high working memory capacity group than for the low working memory capacity group. The positivity following an error (Pe) was modulated to a greater extent by speed‐accuracy instruction for the high working memory capacity group than for the low working memory capacity group. These data help to explicate the neural bases of individual differences in working memory capacity and cognitive control.
Effect of THMs concentrations on the redesigning of Bani Suef water distribution system
Authors: El Shehawy Ragab , El Sayed Abd El Aziz , Mady El Sayed 
Published: March 2017

AbstractControlling trihalomethanes (THMs) formed in water distribution systems (WDS) is an important issue for producing safe drinking water in the last decades. The main objective of this study is to design WDS to minimize THMs formation. Implemented case of Bani Suef water distribution system (BSWDS), Egypt, was modeled and studied for operating scenarios 2020 and 2040 (case study) using WaterCAD software, alternative [1]. Drinking water samples were collected from fifteen points located on BSWDS according to monthly sampling program performed between February and September 2015. Laboratory analysis showed that the total growth rate coefficient was 1.157 day−1, used in THMs prediction by WaterCAD. Three design alternatives [2, 3, 4] were prepared to reduce the formed THMs based on WaterCAD results. Alternative [2] represented new design of alternative [1] through using decreased pipes diameters to study the effect of reducing travel time on minimizing THMs. Alternative [3] was prepared by redesigning alternative [1] using extending new pipelines from dead ends. Alternative [4] was prepared by redesigning alternative [1] through replacing asbestos cement pipes with new UPVC ones. Predicted THMs from alternatives [2, 3, 4] are lower about 27.85, 21.82 and 11.4% than alternative [1], respectively, for 2020. While, for 2040, are lower about 26.41, 20.2 and 11.4%, respectively.
Surface runoff estimation over heterogeneous foothills of Aravalli mountain using medium resolution remote sensing rainfall data with soil conservation system‐curve number method: A case of semi‐arid ungauged Manesar Nala watershed
Authors: Rawat Kishan Singh , Mishra Anil Kumar , Ahmad Nayan 
Published: March 2017

AbstractManesar Nala watershed, having an aerial extent of 71.53 km2, was subjected to modelling of its hydrological behaviour for assessing its water resource potential. Modern tools and techniques of Remote Sensing (RS) and Geographic Information System (GIS) were used for assessment of runoff generating potential using the Hydrologic Soil Cover Complex (HSCC) Method [U.S. Department of Agriculture (USDA)‐Soil Conservation System‐Curve Number (SCS‐CN) approach]. RS and GIS were used in generation and integration of thematic maps [such as Land use/Land cover (using LISS‐III data) and Hydrologic Soil Group (HSG), (using soil map of study area) to derive the Curve Number (CN) for simulating Runoff (Ro)]. The daily rainfall (P) data for the study period 2002–2015 were acquired from NOAA Climate Prediction Center (NCPC). Corresponding Ro from the watershed for intense storm events for 14 years were calculated through RS and GIS. GIS and SCS‐CN model was employed for modelling the runoff production to study its hydrological behaviour. The study showed that the Manesar Nala watershed was having a composite Curve Number – II (CNII) value of 82.5 for normal conditions. For dry and wet conditions these values were estimated at 66.44 (CNI) and 91.56 (CNIII), respectively. This investigation showed that Manesar Nala watershed exhibited an annual average (of 14 years, 2002–2015) Ro volume of 4 542 514.37 m3 based on the average annual rainfall (P) of 0.72 m (720 mm). The average annual surface runoff (Ro) was predicted to be approximately 0.21 m with annual runoff coefficient (CR) of 0.29. During the study, we also found a strong correlation ‘r’ between satellite driven P and Ro from NRCS‐CN method of the order of 0.94. The methodology so developed has the potential to be used in other similar ungauged watersheds in the same agro‐climatic conditions for the purpose of planning of watershed conservation measures and other developmental activities.
Angiotensin II activates CaV1.2 Ca2+ channels through β‐arrestin2 and casein kinase 2 in mouse immature cardiomyocytes
Authors: Kashihara Toshihide , Nakada Tsutomu , Kojima Katsuhiko , Takeshita Toshikazu , Yamada Mitsuhiko 
Published: March 2017

Key points
Angiotensin II (AngII) is crucial in cardiovascular regulation in perinatal mammalians.
Here we show that AngII increases twitch Ca2+ transients of mouse immature but not mature cardiomyocytes by robustly activating CaV1.2 L‐type Ca2+ channels through a novel signalling pathway involving angiotensin type 1 (AT1) receptors, β‐arrestin2 and casein kinase 2.
A β‐arrestin‐biased AT1 receptor agonist, TRV027, was as effective as AngII in activating L‐type Ca2+ channels.
Our results help understand the molecular mechanism by which AngII regulates the perinatal circulation and also suggest that β‐arrestin‐biased AT1 receptor agonists may be valuable therapeutics for paediatric heart failure.
AbstractAngiotensin II (AngII), the main effector peptide of the renin–angiotensin system, plays important roles in cardiovascular regulation in the perinatal period. Despite the well‐known stimulatory effect of AngII on vascular contraction, little is known about regulation of contraction of the immature heart by AngII. Here we found that AngII significantly increased the peak amplitude of twitch Ca2+ transients by robustly activating L‐type CaV1.2 Ca2+ (CaV1.2) channels in mouse immature but not mature cardiomyocytes. This response to AngII was mediated by AT1 receptors and β‐arrestin2. A β‐arrestin‐biased AT1 receptor agonist was as effective as AngII in activating CaV1.2 channels. Src‐family tyrosine kinases (SFKs) and casein kinase 2α’β (CK2α’β) were sequentially activated when AngII activated CaV1.2 channels. A cyclin‐dependent kinase inhibitor, p27Kip1 (p27), inhibited CK2α’β, and AngII removed this inhibitory effect through phosphorylating tyrosine 88 of p27 via SFKs in cardiomyocytes. In a human embryonic kidney cell line, tsA201 cells, overexpression of CK2α’β but not c‐Src directly activated recombinant CaV1.2 channels composed of C‐terminally truncated α1C, the distal C‐terminus of α1C, β2C and α2δ1 subunits, by phosphorylating threonine 1704 located at the interface between the proximal and the distal C‐terminus of CaV1.2α1C subunits. Co‐immunoprecipitation revealed that CaV1.2 channels, CK2α’β and p27 formed a macromolecular complex. Therefore, stimulation of AT1 receptors by AngII activates CaV1.2 channels through β‐arrestin2 and CK2α’β, thereby probably exerting a positive inotropic effect in the immature heart. Our results also indicated that β‐arrestin‐biased AT1 receptor agonists may be used as valuable therapeutics for paediatric heart failure in the future.
Adult and adolescent livestock productive asset transfer programmes to improve mental health, economic stability and family and community relationships in rural South Kivu Province, Democratic Republic of Congo: a protocol of a randomised controlled trial
Authors: Kohli Anjalee , Perrin Nancy A , Remy Mitima Mpanano , Alfred Mirindi Bacikenge , Arsene Kajabika Binkurhorhwa , Nadine Mwinja Bufole , Heri Banyewesize Jean , Clovis Mitima Murhula , Glass Nancy 
Published: March 2017

IntroductionPeople living in poverty have limited access to traditional financial institutions. Microfinance programmes are designed to meet this gap and show promise in improving income, economic productivity and health. Our Congolese–US community academic research partnership developed two livestock productive asset transfer programmes, Pigs for Peace (PFP) and Rabbits for Resilience (RFR), to address the interlinked health, social and economic well-being of individuals, their families and communities. The community-based randomised controlled trials examine the effectiveness of PFP and RFR to improve health, economic stability, and family and community relationships among male and female adults and adolescents living in 10 rural, postconflict villages of eastern Democratic Republic of Congo.Methods and analysisPFP participants include adult permanent residents of rural villages; adolescent participants in RFR include male and female adolescents 10–15 years old living in the selected rural villages. Participants were randomised to intervention or delayed control group. Participants in PFP completed baseline interview prior to intervention and follow-up interview at 6, 12 and 18 months postintervention. In RFR, participants completed baseline interview prior to intervention and follow-up interview at 6, 12 and 18 months postbaseline. The primary outcome of both trials, the change in baseline mental health distress at 18 months in the intervention group (adults, adolescents) compared to control group, is used to calculate sample size.Ethics and disseminationThe Johns Hopkins Medical Institute Internal Review Board approved this protocol. A committee of respected Congolese educators and community members (due to lack of local ethics review board) approved the study. The findings will provide important information on the potential for community-led sustainable development initiatives to build on traditional livelihood (livestock raising, agriculture) to have a sustained health, economic and social impact on the individual, family and community.Trial registration numberNCT02008708,NCT02008695.
212: SCIENTOMETRICS STUDY OF PUBLISHED RETRACTIONS IN PUBMED
Authors: Jalalzadeh Sara , Abbasi Fahimeh , Pashazadeh Fariba 
Published: March 2017

Background and aims:Retractions are published in journals in order to correct, ensure the accuracy and integrity of literature and avoid of misconducts. Hence, retraction is an important mechanism in scientific accreditation especially in health subject. In the recent years, the numbers of retracted scientific articles have been increased but compared with the growth of total publications, it has allocated very small portion. In this study, the retracted articles indexed in PubMed have been assessed.Methods:In this descriptive study, all retracted articles were searched in PubMed with limitation to publication types but without date limitation considered. Then retrieved data were analyzed.Results:The results of this survey showed a total of 9211 retractions published during 1959 to 2016. According to these findings, the least retraction was published in 1959, 1966 and 1973 with one retracted article each year and the most retractions were 871 retractions in 2013. In addition, of 1781 journals published retractions, THE JOURNAL OF BIOLOGICAL CHEMISTRY (298 (3.24%)), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (181 (1.97%)), SCIENCE (NEW YORK, N.Y.) (153 (1.66%)) and NATURE (129 (1.4%)) allocated the most rate of retractions. Furthermore, the assessing of indexing cituation of these findings showed 87.08% were in Medline, 11.26% in PubMed-not-Medline and 0.79% in In-Data-Review. Moreover, the most rate of publication types of retractions were related to “JOURNAL ARTICLE”, “SEARCH SUPPORT, NON-U.S. GOV'T”,” RESEARCH SUPPORT, N.I.H., EXTRAMURAL”, “COMPARATIVE STUDY “and” RANDOMIZED CONTROLLED TRIAL”, also the highest rate of Mesh subject headings in retracted articles were belong to “CELLS, CULTURED”, “OLECULAR SEQUENCE DATA”, “TREATMENT OUTCOME”, “CELL LINE, TUMOR”. Finally, the majority of retracted articles were English (98.5%), then French (0.39%), German (0.33%) and Chinese (0.31%)Conclusion:Retractions publish in two types of self-reported by author or editors discernments. Also two main reasons for retraction are “misconducts” and “unintentional mistakes” of authors that the second reason has the largest numbers of retractions. Therefore, notifying of retracted articles and raising awareness of readers and authors are the best mechanisms for controlling of negative effects of retracted articles. However, publishing of retraction in journals reflects how much importance they are considering to the accuracy of published information.
Validating a fully automated real-time PCR-based system for use in the molecular diagnostic analysis of colorectal carcinoma: a comparison with NGS and IHC
Authors: Colling Richard , Wang Lai Mun , Soilleux Elizabeth 
Published: March 2017

BackgroundMolecular testing is increasingly needed in colorectal carcinoma (CRC) and the current clinically relevant mutations are inBRAF,KRASandNRAS. This study aimed to further validate a new alternative polymerase chain reaction (PCR) platform (Idylla, Biocartis) against existing next-generation sequencing (NGS) and immunohistochemistry (IHC) assays.Methods56 Idylla tests were performed on 43 CRC cases, in a total of 74 comparisons against an NGS panel (Ion Torrent) and the VE1 (anti-BRAF) antibody IHC. Discrepant cases were also compared with either conventional (Cobas) or droplet digital PCR (Bio-Rad).ResultsIdylla showed an overall concordance of 100% (95% CI 93% to 100%) with comparator molecular testing and indications were that Idylla is likely to be more sensitive than routine NGS.BRAFIHC showed 90% concordance with NGS (95% CI 70% to 97%).ConclusionsThis study validates Idylla in formalin-fixed, paraffin-embedded CRC tissue.BRAFIHC, however, is an unreliable substitute for molecular testing in CRC.
Differential processing of the direction and focus of expansion of optic flow stimuli in areas MST and V3A of the human visual cortex
Authors: Strong Samantha L. , Silson Edward H. , Gouws André D. , Morland Antony B. , McKeefry Declan J. 
Published: March 2017

Human neuropsychological and neuroimaging studies have raised the possibility that different attributes of optic flow stimuli, namely radial direction and the position of the focus of expansion (FOE), are processed within separate cortical areas. In the human brain, visual areas V5/MT+ and V3A have been proposed as integral to the analysis of these different attributes of optic flow stimuli. To establish direct causal relationships between neural activity in human (h)V5/MT+ and V3A and the perception of radial motion direction and FOE position, we used transcranial magnetic stimulation (TMS) to disrupt cortical activity in these areas while participants performed behavioral tasks dependent on these different aspects of optic flow stimuli. The cortical regions of interest were identified in seven human participants using standard functional MRI retinotopic mapping techniques and functional localizers. TMS to area V3A was found to disrupt FOE positional judgments but not radial direction discrimination, whereas the application of TMS to an anterior subdivision of hV5/MT+, MST/TO-2 produced the reverse effects, disrupting radial direction discrimination but eliciting no effect on the FOE positional judgment task. This double dissociation demonstrates that FOE position and radial direction of optic flow stimuli are signaled independently by neural activity in areas hV5/MT+ and V3A. NEW & NOTEWORTHY Optic flow constitutes a biologically relevant visual cue as we move through any environment. With the use of neuroimaging and brain-stimulation techniques, this study demonstrates that separate human brain areas are involved in the analysis of the direction of radial motion and the focus of expansion in optic flow. This dissociation reveals the existence of separate processing pathways for the analysis of different attributes of optic flow that are important for the guidance of self-locomotion and object avoidance.
Ion diffusion may introduce spurious current sources in current-source density (CSD) analysis
Authors: Halnes Geir , Mäki-Marttunen Tuomo , Pettersen Klas H. , Andreassen Ole A. , Einevoll Gaute T. 
Published: March 2017

Current-source density (CSD) analysis is a well-established method for analyzing recorded local field potentials (LFPs), that is, the low-frequency part of extracellular potentials. Standard CSD theory is based on the assumption that all extracellular currents are purely ohmic, and thus neglects the possible impact from ionic diffusion on recorded potentials. However, it has previously been shown that in physiological conditions with large ion-concentration gradients, diffusive currents can evoke slow shifts in extracellular potentials. Using computer simulations, we here show that diffusion-evoked potential shifts can introduce errors in standard CSD analysis, and can lead to prediction of spurious current sources. Further, we here show that the diffusion-evoked prediction errors can be removed by using an improved CSD estimator which accounts for concentration-dependent effects. NEW & NOTEWORTHY Standard CSD analysis does not account for ionic diffusion. Using biophysically realistic computer simulations, we show that unaccounted-for diffusive currents can lead to the prediction of spurious current sources. This finding may be of strong interest for in vivo electrophysiologists doing extracellular recordings in general, and CSD analysis in particular.
A Fas Ligand (FasL)-Fused Humanized Antibody Against Tumor-Associated Glycoprotein 72 Selectively Exhibits the Cytotoxic Effect Against Oral Cancer Cells with a Low FasL/Fas Ratio
Authors: Chien Ming-Hsien , Chang Wei-Min , Lee Wei-Jiunn , Chang Yu-Chan , Lai Tsung-Ching , Chan Derek V. , Sharma Rahul , Lin Yuan-Feng , Hsiao Michael 
Published: March 2017

Abstract
Altered expression of the Fas ligand (FasL)/Fas ratio exhibits a direct impact on the prognosis of cancer patients, and its impairment in cancer cells may lead to apoptosis resistance. Thus, the development of effective therapies targeting the FasL/Fas system may play an important role in the fight against cancer. In this study, we evaluated whether a fusion protein (hcc49scFv-FasL) comprising of the cytotoxicity domain of the FasL fused to a humanized antibody (CC49) against tumor-associated glycoprotein 72, which is expressed on oral squamous cell carcinoma (OSCC), can selectively kill OSCC cells with different FasL/Fas ratios. In clinical samples, the significantly low FasL and high Fas transcripts were observed in tumors compared with normal tissues. A lower FasL/Fas ratio was correlated with a worse prognosis of OSCC patients and higher proliferative and invasive abilities of OSCC cells. The hcc49scFv-FasL showed a selective cytotoxic effect on OSCC cells (Cal-27 and SAS) but not on normal oral keratinocytes cells (HOK) through apoptosis induction. Moreover, SAS cells harboring a lower FasL/Fas ratio than Cal-27 were more sensitive to the cytotoxic effect of hcc49scFv-FasL. Unlike wild-type FasL, hcc49scFv-FasL was not cleaved by matrix metalloproteinases and did not induce nonapoptotic signaling in SAS cells. In vivo, we found that hcc49scFv-FasL drastically reduced the formation of lymph node metastasis and decreased primary tumor growth in SAS orthotopic and subcutaneous xenograft tumor models. Collectively, our data indicate that a tumor-targeting antibody fused to the FasL can be a powerful tool for OSCC treatment, especially in populations with a low FasL/Fas ratio. Mol Cancer Ther; 16(6); 1102–13. ©2017 AACR.
Disruption of TCF/β-Catenin Binding Impairs Wnt Signaling and Induces Apoptosis in Soft Tissue Sarcoma Cells
Authors: Martinez-Font Esther , Felipe-Abrio Irene , Calabuig-Fariñas Silvia , Ramos Rafael , Terrasa Josefa , Vögler Oliver , Alemany Regina , Martín-Broto Javier , Obrador-Hevia Antònia 
Published: March 2017

AbstractSoft tissue sarcomas (STS) are malignant tumors of mesenchymal origin and represent around 1% of adult cancers, being a very heterogeneous group of tumors with more than 50 different subtypes. The Wnt signaling pathway is involved in the development and in the regulation, self-renewal, and differentiation of mesenchymal stem cells, and plays a role in sarcomagenesis. In this study, we have tested pharmacologic inhibition of Wnt signaling mediated by disruption of TCF/β-catenin binding and AXIN stabilization, being the first strategy more efficient in reducing cell viability and downstream effects. We have shown that disruption of TCF/β-catenin binding with PKF118-310 produces in vitro antitumor activity in a panel of prevalent representative STS cell lines and primary cultures. At the molecular level, PKF118-310 treatment reduced β-catenin nuclear localization, reporter activity, and target genes, resulting in an increase in apoptosis. Importantly, combination of PKF118-310 with doxorubicin resulted in enhanced reduction of cell viability, suggesting that Wnt inhibition could be a new combination regime in these patients. Our findings support the usefulness of Wnt inhibitors as new therapeutic strategies for the prevalent STS. Mol Cancer Ther; 16(6); 1166–76. ©2017 AACR.