bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture
Authors: Watanabe Daisuke , Koyanagi-Aoi Michiyo , Taniguchi-Ikeda Mariko , Yoshida Yukiko , Azuma Takeshi , Aoi Takashi 
Published: November 2017

Abstract
γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways in a MHC-unrestricted manner. Thus, immunotherapy using γδT cells is considered to be effective for various types of cancer. Occasionally, however, ex vivo expanded cells are not as effective as expected due to cell exhaustion. To overcome the issue of T-cell exhaustion, researchers have generated induced pluripotent stem cells (iPSCs) that harbor the same T-cell receptor (TCR) genes as their original T-cells, which provide nearly limitless sources for antigen-specific cytotoxic T lymphocytes (CTLs). However, these technologies have focused on αβT cells and require a population of antigen-specific CTLs, which are purified by cell sorting with HLA-peptide multimer, as the origin of iPS cells. In the present study, we aimed to develop an efficient and convenient system for generating iPSCs that harbor rearrangements of the TCRG and TCRD gene regions (γδT-iPSCs) without cell-sorting. We stimulated human whole peripheral blood mononuclear cell (PBMC) culture using Interleukin-2 and Zoledronate to activate γδT cells. Gene transfer into those cells with the Sendai virus vector resulted in γδT cell-dominant expression of exogenous genes. The introduction of reprogramming factors into the stimulated PBMC culture allowed us to establish iPSC lines. Around 70% of the established lines carried rearrangements at the TCRG and TCRD gene locus. The γδT-iPSCs could differentiate into hematopoietic progenitors. Our technology will pave the way for new avenues toward novel immunotherapy that can be applied for various types of cancer.
A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta-Thalassemia and Sickle Cell Disease
Authors: Cai Liuhong , Bai Hao , Mahairaki Vasiliki , Gao Yongxing , He Chaoxia , Wen Yanfei , Jin You-Chuan , Wang You , Pan Rachel L. , Qasba Armaan , Ye Zhaohui , Cheng Linzhao 
Published: November 2017

Abstract
Beta-thalassemia is one of the most common recessive genetic diseases, caused by mutations in the HBB gene. Over 200 different types of mutations in the HBB gene containing three exons have been identified in patients with β-thalassemia (β-thal) whereas a homozygous mutation in exon 1 causes sickle cell disease (SCD). Novel therapeutic strategies to permanently correct the HBB mutation in stem cells that are able to expand and differentiate into erythrocytes producing corrected HBB proteins are highly desirable. Genome editing aided by CRISPR/Cas9 and other site-specific engineered nucleases offers promise to precisely correct a genetic mutation in the native genome without alterations in other parts of the human genome. Although making a sequence-specific nuclease to enhance correction of a specific HBB mutation by homology-directed repair (HDR) is becoming straightforward, targeting various HBB mutations of β-thal is still challenging because individual guide RNA as well as a donor DNA template for HDR of each type of HBB gene mutation have to be selected and validated. Using human induced pluripotent stem cells (iPSCs) from two β-thal patients with different HBB gene mutations, we devised and tested a universal strategy to achieve targeted insertion of the HBB cDNA in exon 1 of HBB gene using Cas9 and two validated guide RNAs. We observed that HBB protein production was restored in erythrocytes derived from iPSCs of two patients. This strategy of restoring functional HBB gene expression will be able to correct most types of HBB gene mutations in β-thal and SCD.
Lignin Exhibits Recalcitrance‐Associated Features Following the Consolidated Bioprocessing of Populus trichocarpa Natural Variants
Authors: Akinosho Hannah , Yee Kelsey , Rodriguez Miguel , Muchero Wellington , Yoo Chang Geun , Li Mi , Thompson Olivia , Pu Yunqiao , Brown Steven , Mielenz Johnathan , Ragauskas Arthur J. 
Published: November 2017

AbstractBecause cellulosic ethanol production remains cost‐prohibitive„ advances in consolidated bioprocessing (CBP) have been directed towards lifting this restriction. CBP reduces the need for added enzymes and can potentially slash ethanol production costs through process integration. Clostridium thermocellum, a CBP microorganism, organizes its enzymes in a multi‐enzyme complex ‐ a stark contrast to fungal enzymes. Nonetheless, recalcitrance may limit the extent of biomass deconstruction. Herein, six Populus were treated with C. thermocellum (ATCC 27405) and characterized to determine structural changes that resulted from CBP. The 2D HSQC NMR spectra of lignin‐enriched residues revealed that higher S/G ratio (2.6) and fewer carbon‐carbon interunit linkages (generally 2–5%) were present in the top performing poplar. Furthermore, cellulose degree of polymerization data suggests that C. thermocellum likely circumvents long chain cellulose, while cellulose crystallinity and hemicellulose molecular weight data do not provide a direct indication of features connected to recalcitrance. Hence, C. thermocellum is similarly impacted by the proposed lignin properties that negatively impact biomass deconstruction using fungal enzymes.
Chloro‐Bridged Hexanuclear Pd(II) Clusters Supported by cis‐Blocking Tris(imido)phosphate Trianions
Authors: Gupta Arvind K. , Reddy Shada Arun Dixith , Rajasekar Prabhakaran , Prakash Prabhat , Boomishankar Ramamoorthy 
Published: November 2017

AbstractTransition metal complexes derived from anionic polyimido ligands are very limited in the literature. Here we describe the deprotonation of alkyl substituted phosphoric triamides with bis(benzonitrile)palladium dichloride which results in the formation of chloro‐bridged hexanuclear palladium(II) clusters of formula {Pd3[(NR)3PO](Cl)3}2 in octahedral and prismatic geometries. These clusters consist of two trinuclear Pd3‐motifs, each cis‐protected by a tris(alkylimido)phosphate trianion, which are linked together by six μ2‐Cl bridges. A staggered arrangement of the two imido‐Pd3 units results in the formation of octahedral clusters (R=iBu, nBu, cHex, iPr and nPr), while an eclipsed conformation (R=tBu) gives rise to a prismatic cluster. A similar prismatic configuration was obtained for the mixed‐bridged clusters {Pd3[(NR)3PO](OAc)2(Cl)}2 (R=cHex and iPr), formed in the reaction involving the corresponding phosphoric triamide, Pd(OAc)2 and Pd(PhCN)2Cl2. The reaction of an octahedral cluster {Pd3[(NnPr)3PO](Cl)3}2 with 1,1’‐ferrocene dicarboxylic acid (FDC−H) gave the prismatic cluster {Pd3[(NnPr)3PO](FDC)(Cl)}2 which again consists of a mixed‐bridged environment with FDC and Cl− linkers. Further, treatment of the octahedral {Pd3[(NnPr)3PO](Cl)3}2 cluster with iPrNH2, a stronger nucleophile, leads to its symmetric cleavage and forms the trinuclear cluster {Pd3[(NR)3PO]Cl3(R'NH2)3}. We have also shown the catalytic activity of some of these Pd(II) complexes in the Suzuki reactions in both organic and aqueous phase conditions which suggest that all these imido‐Pd(II) clusters are still suitable for catalytic reactions.
Syn/Anti Pair of Triazole‐Bridged Cryptophanes: Synthesis, Characterization with Crystal Structures
Authors: Satha Pardhasaradhi , Illa Giri Teja , Hazra Sohan , Purohit Chandra Shekhar 
Published: November 2017

AbstractA pair of triazole bridged‐cryptophanes have been synthesized by template method. The two diastereomers of the cryptophane, syn‐ and anti‐isomers were isolated and characterized using NMR and Mass spectroscopy. Further confirmation of stereo‐chemical configuration was done using Single Crystal analysis. The syn isomer was shown to encapsulate tetraethyl ammonium cation suggested by NMR, HRMS and confirmed by crystal structure.
Preparation of Zeolite F as Slow Release Fertilizers from K‐Feldspar Powder
Authors: Yuan Jiangyan , Yang Jing , Ma Hongwen , Chang Qianqian 
Published: November 2017

AbstractInfluence of KOH concentration and reaction time on the synthesis of zeolite F from K‐feldspar, and the kinetics of potassium slow release from the product were studied. X‐ray diffraction (XRD), Fourier transformation infrared spectrometry (FTIR), and scanning electron microscopy (SEM) were applied to characterize the as‐synthesized samples. The XRD results indicate that there exist K−G zeolite (K2Al2SiO6⋅H2O) with hexagonal structure at the KOH concentration of 2 mol⋅kg−1 and zeolite F (KAlSiO4⋅1.5H2O) with tetragonal structure at 4–5 mol⋅kg−1. In terms of synthesis efficiency, crystallization time of 12 h was chosen as optimal condition. The surface area and pore diameter of zeolite F sample synthesized in 4 mol⋅kg−1 at 95 °C for 12 h were investigated by surface area analyzer, the values of which are 27 m2/g and 0.513 nm, respectively. Slow‐release testing results suggest that the potassium cumulative release fraction of zeolite F in water for 42 d is up to 68.97% and can be fitted well with Elovich equation.
Three Gd‐Based Metal‐Organic Frameworks Constructed from Similar Dicarboxylate Ligands with Large Magnetic Entropy Changes
Authors: Yao Shu‐Li , Cao Chen , Tian Xue‐Mei , Zheng Teng‐Fei , Liu Sui‐Jun , Tong Xiao‐Lan , Liao Jin‐Sheng , Chen Jing‐Lin , Wen He‐Rui 
Published: November 2017

AbstractThree Gd‐based metal‐organic frameworks with the formulas of {[Gd2(suc)3(H2O)2]⋅H2O}∞ (1), {[Gd2(fum)3(H2O)4]⋅2H2O}∞ (2) and [Gd(glu)(ox)0.5(H2O)2]∞ (3) (suc=succinate, fum=fumarate, glu=glutarate and ox=oxalate) have been hydro/solvothermally synthesized through the reaction of Gd2O3 and dicarboxylate ligands. Complexes 1 and 2 take three‐dimensional (3D) structures derived from carboxylate‐bridged chains, while complex 3 presents a Zigzag chain‐based two‐dimensional (2D) structure. All of them exhibit relatively good thermal stabilities. Magnetic investigations indicate that complexes 1−3 reveal weak magnetic interactions between the neighbouring GdIII ions. Moreover, they display large magnetic entropy changes with −ΔSmmax reaching 42.22 J kg−1 K−1 (1), 37.97 J kg−1 K−1 (2) and 39.15 J kg−1 K−1 (3) with ΔH=7 T, respectively, indicating that they are promising cryogenic magnetorefrigerants.
Synthesis of Dimeric Imidazo[1, 5‐a]pyridines and Their Photophysical Properties
Authors: Yagishita Fumitoshi , Kozai Natsumi , Nii Chiho , Tezuka Yoshihiko , Uemura Naohiro , Yoshida Yasushi , Mino Takashi , Sakamoto Masami , Kawamura Yasuhiko 
Published: November 2017

AbstractWe designed and synthesized dimeric imidazo[1, 5‐a]pyridines possessing various aryl groups at 1,1’‐positions. The solution of these derivatives exhibited fluorescence emission in wavelength range of 487–512 nm. The fluorescence emissions were slightly red‐shifted under acidic condition by addition of trifluoroacetic acid. Furthermore, the original fluorescence spectra were observed after the addition of triethylamine as a base.
Solution‐Processed Wide‐Bandgap Organic Semiconductor Nanostructures Arrays for Nonvolatile Organic Field‐Effect Transistor Memory
Authors: Li Wen , Guo Fengning , Ling Haifeng , Liu Hui , Yi Mingdong , Zhang Peng , Wang Wenjun , Xie Linghai , Huang Wei 
Published: November 2017

AbstractIn this paper, the development of organic field‐effect transistor (OFET) memory device based on isolated and ordered nanostructures (NSs) arrays of wide‐bandgap (WBG) small‐molecule organic semiconductor material [2‐(9‐(4‐(octyloxy)phenyl)‐9H‐fluoren‐2‐yl)thiophene]3 (WG3) is reported. The WG3 NSs are prepared from phase separation by spin‐coating blend solutions of WG3/trimethylolpropane (TMP), and then introduced as charge storage elements for nonvolatile OFET memory devices. Compared to the OFET memory device with smooth WG3 film, the device based on WG3 NSs arrays exhibits significant improvements in memory performance including larger memory window (≈45 V), faster switching speed (≈1 s), stable retention capability (>104 s), and reliable switching properties. A quantitative study of the WG3 NSs morphology reveals that enhanced memory performance is attributed to the improved charge trapping/charge‐exciton annihilation efficiency induced by increased contact area between the WG3 NSs and pentacene layer. This versatile solution‐processing approach to preparing WG3 NSs arrays as charge trapping sites allows for fabrication of high‐performance nonvolatile OFET memory devices, which could be applicable to a wide range of WBG organic semiconductor materials.
Single Pixel Black Phosphorus Photodetector for Near‐Infrared Imaging
Authors: Miao Jinshui , Song Bo , Xu Zhihao , Cai Le , Zhang Suoming , Dong Lixin , Wang Chuan 
Published: November 2017

AbstractInfrared imaging systems have wide range of military or civil applications and 2D nanomaterials have recently emerged as potential sensing materials that may outperform conventional ones such as HgCdTe, InGaAs, and InSb. As an example, 2D black phosphorus (BP) thin film has a thickness‐dependent direct bandgap with low shot noise and noncryogenic operation for visible to mid‐infrared photodetection. In this paper, the use of a single‐pixel photodetector made with few‐layer BP thin film for near‐infrared imaging applications is demonstrated. The imaging is achieved by combining the photodetector with a digital micromirror device to encode and subsequently reconstruct the image based on compressive sensing algorithm. Stationary images of a near‐infrared laser spot (λ = 830 nm) with up to 64 × 64 pixels are captured using this single‐pixel BP camera with 2000 times of measurements, which is only half of the total number of pixels. The imaging platform demonstrated in this work circumvents the grand challenges of scalable BP material growth for photodetector array fabrication and shows the efficacy of utilizing the outstanding performance of BP photodetector for future high‐speed infrared camera applications.
Manganese Dioxide Coated WS2@Fe3O4/sSiO2 Nanocomposites for pH‐Responsive MR Imaging and Oxygen‐Elevated Synergetic Therapy
Authors: Yang Guangbao , Zhang Rui , Liang Chao , Zhao He , Yi Xuan , Shen Sida , Yang Kai , Cheng Liang , Liu Zhuang 
Published: November 2017

AbstractRecently, the development of multifunctional theranostic nanoplatforms to realize tumor‐specific imaging and enhanced cancer therapy via responding or modulating the tumor microenvironment (TME) has attracted tremendous interests in the field of nanomedicine. Herein, tungsten disulfide (WS2) nanoflakes with their surface adsorbed with iron oxide nanoparticles (IONPs) via self‐assembly are coated with silica and then subsequently with manganese dioxide (MnO2), on to which polyethylene glycol (PEG) is attached. The obtained WS2‐IO/S@MO‐PEG appears to be highly sensitive to pH, enabling tumor pH‐responsive magnetic resonance imaging with IONPs as the pH‐inert T2 contrast probe and MnO2 as the pH‐sensitive T1 contrast probe. Meanwhile, synergistic combination tumor therapy is realized with such WS2‐IO/S@MO‐PEG, by utilizing the strong near‐infrared light and X‐ray absorbance of WS2 for photothermal therapy (PTT) and enhanced cancer radiotherapy (RT), respectively, as well as the ability of MnO2 to decompose tumor endogenous H2O2 and relieve tumor hypoxia to further overcome hypoxia‐associated radiotherapy resistance. The combination of PTT and RT with WS2‐IO/S@MO‐PEG results in a remarkable synergistic effect to destruct tumors. This work highlights the promise of developing multifunction nanocomposites for TME‐specific imaging and TME modulation, aiming at precision cancer synergistic treatment.
Carbon Necklace Incorporated Electroactive Reservoir Constructing Flexible Papers for Advanced Lithium–Ion Batteries
Authors: Du Min , Rui Kun , Chang Yuanqin , Zhang Yu , Ma Zhongyuan , Sun Wenping , Yan Qingyu , Zhu Jixin , Huang Wei 
Published: November 2017

AbstractMetal–organic frameworks (MOFs) and their derivatives with well‐defined structures and compositions show great potential for wide applications such as sensors, catalysis, energy storage, and conversion, etc. However, poor electric conductivity and large volume expansion are main obstacles for their utilization in energy storage, e.g., lithium–ion batteries and supercapacitors. Herein, a facile strategy is proposed for embedding the MOFs, e.g., ZIF‐67 and MIL‐88 into polyacrylonitrile fibers, which is further used as a template to build a 3D interconnected conductive carbon necklace paper. Owing to the unique structure features of good electric conductivity, interconnected frameworks, electroactive reservoir, and dual dopants, the obtained flexible electrodes with no additives exhibit high specific capacities, good rate capability, and prolonged cycling stability. The hollow dodecahedral ZIF‐67 derived carbon necklace paper delivers a high specific capacity of 1200 mAh g−1 and superior stability of more than 400 cycles without capacity decay. Moreover, the spindle‐like MIL‐88 derived carbon necklace paper shows a high reversible capacity of 980 mAh g−1. Their unique 3D interconnected structure and outstanding electrochemical performance pave the way for extending the MOF‐based interweaving materials toward potential applications in portable and wearable electronic devices.
Cyclic loading tests and shear strength of steel reinforced recycled concrete beam–column inner joints
Authors: Xue Jianyang , Zhai Lei , Bao Yuze , Ren Rui , Zhang Xicheng 
Published: November 2017

SummaryTo study the seismic behavior and shear strength of steel reinforced recycled concrete (SRRC) beam–column inner joints, four 1:2.5 scaled specimens with different replacement percentage of recycled coarse aggregates were fabricated and tested under cyclic lateral loadings. The failure modes of SRRC joints were observed, and the various mechanical indexes of SRRC joints, including hysteresis loops, envelope curves, load carrying capacity, ductility, energy dissipation capacity, and stiffness degradation, were analyzed. The results indicate that the main failure mode of the SRRC joints is the shearing diagonal compression in the core zone of joint. The seismic behavior of the SRRC inner joints degrade only slightly compared with the ordinary steel reinforced concrete inner joints. Based on the test and analysis results, a modified design method is proposed to calculate the nominal shear strength of SRRC inner joints.
Lessons Learned: Applying Principles of Reading Recovery in the Classroom
Authors: Lewis Kayla 
Published: November 2017

AbstractIn this article, one literacy coach turned university professor describes her journey through Reading Recovery training and the lessons she learned along the way. She reflects on her teaching before and after studying Clay's work, and she describes the changes that she went through as a teacher as she applied the principles to her own teaching and to her work as a literacy coach. Teachers can learn how to apply these nine lessons learned, including the importance of scaffolding and of working within the zone of proximal development, in their own work with struggling readers in the classroom.
Dispersion properties of inhomogeneous poroelastic layer
Authors: Gusakov Dmitry , Vatul'yan Alexander 
Published: November 2017

AbstractThis paper deals with the problem of free oscillations of a transversely‐isotropic inhomogeneous poroelastic layer. Dynamic behaviour of a poroelastic layer is modeled using linear Biot's consolidation theory. The problem is described by a system of linear differential equations with complex coefficients. We reduce this system to an equivalent system of double dimension with real coefficients. The dispersion relation is obtained using the shooting method. In order to obtain analytical approximation for the first curves of the dispersion spectrum, the Galerkin and Ritz methods are used. Structural features of the dispersion spectrum for an inhomogeneous transversely‐isotropic poroelastic layer are described. The dependence of the form of the dispersion spectrum on the inhomogeneity type is revealed. The comparative analysis of the numerical and analytical methods is provided.
Radioembolization Versus Bland Embolization for Hepatic Metastases from Small Intestinal Neuroendocrine Tumors: Short‐Term Results of a Randomized Clinical Trial
Authors: Elf Anna‐Karin , Andersson Mats , Henrikson Olof , Jalnefjord Oscar , Ljungberg Maria , Svensson Johanna , Wängberg Bo , Johanson Viktor 
Published: November 2017

AbstractBackgroundRadioembolization (RE) with intra‐arterial administration of 90Y microspheres is a promising technique for the treatment of liver metastases from small intestinal neuroendocrine tumors (SI‐NET) not amenable to surgery or local ablation. However, studies comparing RE to other loco‐regional therapies are lacking. The aim of this randomized study was to compare the therapeutic response and safety after RE and bland hepatic arterial embolization (HAE), and to investigate early therapy‐induced changes with diffusion‐weighted MRI (DWI‐MRI).MethodsEleven patients were included in a prospective randomized controlled pilot study, six assigned to RE and five to HAE. Response according to RECIST 1.1 using MRI or CT at 3 and 6 months post‐treatment was recorded as well as changes in DWI‐MRI parameters after 1 month. Data on biochemical tumor response, toxicity, and side effects were also collected.ResultsThree months after treatment, all patients in the HAE group showed partial response according to RECIST while none in the RE group did (p = 0.0022). After 6 months, the response rates were 4/5 (80%) and 2/6 (33%) in the HAE and RE groups, respectively (NS). DWI‐MRI metrics could not predict RECIST response, but lower pretreatment ADC(120–800) and larger ADC(0–800) increase at 1 month were related to larger decrease in tumor diameter when all tumors were counted.ConclusionHAE resulted in significantly higher RECIST response after 3 months, but no difference compared to RE remained after 6 months. These preliminary findings indicate that HAE remains a safe option for the treatment of liver metastases from SI‐NET, and further studies are needed to establish the role of RE and the predictive value of MR‐DWI.
Growth of Focal Nodular Hyperplasia is Not a Reason for Surgical Intervention, but Patients Should be Referred to a Tertiary Referral Centre
Authors: Bröker Mirelle E. E. , Klompenhouwer Anne J. , Gaspersz Marcia P. , Alleleyn Annick M. E. , Dwarkasing Roy S. , Pieters Indra C. , de Man Robert A. , IJzermans Jan N. M. 
Published: November 2017

AbstractBackgroundWhen a liver lesion diagnosed as focal nodular hyperplasia (FNH) increases in size, it may cause doubt about the initial diagnosis. In many cases, additional investigations will follow to exclude hepatocellular adenoma or malignancy. This retrospective cohort study addresses the implications of growth of FNH for clinical management.MethodsWe included patients diagnosed with FNH based on ≥2 imaging modalities between 2002 and 2015. Characteristics of patients with growing FNH with sequential imaging in a 6‐month interval were compared to non‐growing FNH.ResultsGrowth was reported in 19/162 (12%) patients, ranging from 21 to 200%. Resection was performed in 4/19 growing FNHs; histological examination confirmed FNH in all patients. In all 15 conservatively treated patients, additional imaging confirmed FNH diagnosis. No adverse outcomes were reported. No differences were found in characteristics and presentation of patients with growing or non‐growing FNH.ConclusionThis study confirms that FNH may grow significantly without causing symptoms. A significant increase in size should not have any implications on clinical management if confident diagnosis by imaging has been established by a tertiary benign liver multidisciplinary team. Liver biopsy is only indicated in case of doubt after state‐of‐the‐art imaging. Resection is deemed unnecessary if the diagnosis is confirmed by multiple imaging modalities in a tertiary referral centre.