bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fluorescence polarization assay for bisphenol analogs in soybean oil using glucocorticoid receptor
Authors: Guan Tianzhu , Sun Yonghai , Yu Hansong , Li Tiezhu , Zhang Jie , Zhang Tiehua 
Published: March 2017

A fluorescence polarization (FP) assay based on receptor was developed for the determination of bisphenol analogs (BPs) in soybean oil. In the competitive binding system, dexamethasone fluorescein (Dex‐fl) and glucocorticoid receptor (GR) were selected as probe and recognition element, respectively. Crucial factors that affect the precision and accuracy were optimized. After development, the method was characterized by IC50 values ranging from 1.8 to 66.9 µM of 11 BPs. The established method revealed a limit of detection (LOD) of 1.0 µM and a working range of 7.6–30 µM for BPA in soybean oil. Analysis of spiked soybean oil samples showed that acceptable recoveries ranged from 71.99 to 82.95% with coefficients of variation (CVs) less than 10%. Furthermore, the results detected by the FP assay showed good agreement with that of HPLC with a fit of R2 = 0.9171. It is concluded that this method offers a reliable alternative for determination of BPs in soybean oil.Practical applications: This work aims to develop FP assay for determination of BPs in soybean oil. Based on the recombinant GR, this method can detect multiple BPs simultaneously. It can potentially be used for high‐throughput screening of BPs, which may serve as an assistant of chromatographic techniques.
Energy detection–based spectrum sensing with constraint region in cognitive LTE systems
Authors: Wang Nan , Gao Yue , Yang Fengyi , Bi Qi , Xie Weiliang , Parini Clive 
Published: March 2017

AbstractCognitive LTE system, such as LTE‐U, has received tremendous interest from almost all research disciplines in wireless communications. In Cognitive LTE systems, spectrum sensing is the key enabling technology to realize dynamic spectrum access. In this paper, an error decision probability is proposed to solve the trade‐off problem between detection and false alarm probability with the constraint region requirement. The closed‐form expression for the error decision probability, satisfied Signal‐to‐Noise ratio value, number of samples, and primary users' spectrum utilization ratio are derived for in both fixed and adaptive threshold setting algorithms. By implementing both Welch and wavelet‐based energy detectors, the adaptive threshold setting algorithm demonstrates a more reliable and robust sensing result for both primary users and secondary users in comparison with the conventional fixed one. Furthermore, the wavelet denoising method is applied to improve the sensing performance when there are insufficient number of samples.
Non‐Hodgkin lymphoma in South East Asia: An analysis of the histopathology, clinical features, and survival from Thailand
Authors: Intragumtornchai Tanin , Bunworasate Udomsak , Wudhikarn Kitsada , Lekhakula Arnuparp , Julamanee Jakrawadi , Chansung Kanchana , Sirijerachai Chittima , Norasetthada Lalita , Nawarawong Weerasak , Khuhapinant Archrob , Siritanaratanakul Noppadol , Numbenjapon Tontanai , Prayongratana Kannadit , Chuncharunee Suporn , Niparuck Pimjai , Suwanban Tawatchai , Kanitsap Nongluk , Wongkhantee Somchai , Pornvipavee Rutchanid , Wong Peerapon , Makruasi Nisa , Wannakrairot Pongsak , Assanasen Thamathorn , Sukpanichnant Sanya , Boonsakan Paisarn , Kanoksil Wasana , Ya‐in Charin , Kayasut Kanita , Mitranun Winyu , Warnnissorn Naree 
Published: March 2017

AbstractSystemic reports on the descriptive epidemiology of non‐Hodgkin lymphoma (NHL) from Southeast Asia are scarce. A nationwide multi‐institutional registry was conducted to compare the histopathology, clinical features, and survival of Thai adult patients with NHL using large registries, especially those from Far East Asia (FEA). Using a web‐based registry system, 13 major medical centers from the 4 geographic regions of Thailand prospectively collected, from 2007 to 2014, the diagnostic pathology, according to the World Health Organization classification, 2008, clinical features and survival of 4056 patients who were newly diagnosed with NHL. The median age of the patients was 56 years (range, 16‐99 years). The male‐to‐female ratio was 1.3:1. From the total of 4056 patients, T/NK‐cell lymphoma (TNKCL) accounted for 12.6% of cases, and 5.1% had human immunodeficiency virus–associated lymphoma. The four leading histological subtypes were diffuse large B‐cell lymphoma, not otherwise specified (58.1%); follicular lymphoma (5.6%); extranodal mucosa‐associated lymphoid tissue lymphoma (5.2%); and peripheral T‐cell lymphoma, not otherwise specified (4.0%). With a median follow‐up duration of 46.1 months, the median overall survival of B‐cell NHL was significantly longer than that of patients with TNKCL (76.5 vs 28.8 months, P = .0001). Compared to FEA, the Thai registry had an approximately one‐half lower relative frequency of TNKCL; the prevalence of extranodal mucosa‐associated lymphoid tissue lymphoma was much lower than in Korea, and the frequency of extranodal TNKCL, nasal type, was strikingly low compared to China. It is concluded that while the median age of Thai patients with NHL was approximately a decade younger than for Caucasians, the long‐term survival rates for most histological subtypes were comparable. While the histological distribution generally complied with the characteristic Asian features, some differences from FEA were observed.
Change management in an environment of ongoing primary health care system reform: A case study of Australian primary health care services
Authors: Javanparast Sara , Maddern Janny , Baum Fran , Freeman Toby , Lawless Angela , Labonté Ronald , Sanders David 
Published: March 2017

SummaryIntroduction/BackgroundGlobally, health reforms continue to be high on the health policy agenda to respond to the increasing health care costs and managing the emerging complex health conditions. Many countries have emphasised PHC to prevent high cost of hospital care and improve population health and equity. The existing tension in PHC philosophies and complexity of PHC setting make the implementation and management of these changes more difficult. This paper presents an Australian case study of PHC restructuring and how these changes have been managed from the viewpoint of practitioners and middle managers.MethodsAs part of a 5‐year project, we interviewed PHC practitioners and managers of services in 7 Australian PHC services.FindingsOur findings revealed a policy shift away from the principles of comprehensive PHC including health promotion and action on social determinants of health to one‐to‐one disease management during the course of study. Analysis of the process of change shows that overall, rapid, and top‐down radical reforms of policies and directions were the main characteristic of changes with minimal communication with practitioners and service managers. The study showed that services with community‐controlled model of governance had more autonomy to use an emergent model of change and to maintain their comprehensive PHC services.ConclusionsChange is an inevitable feature of PHC systems continually trying to respond to health care demand and cost pressures. The implementation of change in complex settings such as PHC requires appropriate change management strategies to ensure that the proposed reforms are understood, accepted, and implemented successfully.
Evidence for persistent organochlorine pollutants in the human adrenal cortex
Authors: Fommei Enza , Turci Roberta , Ripoli Andrea , Balzan Silvana , Bianchi Fabrizio , Morelli Luca , Coi Alessio 
Published: March 2017

AbstractEnvironmental pollutants may act as endocrine disruptors in animals. Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) enter the food chain and may accumulate in the fatty animal tissues, including adrenals. To our knowledge, no previous study has investigated their presence in the human normal adrenal (NA) cortex and aldosterone‐producing adenomas (APA). Surgical fragments of APA from 11 patients and NA from 8 kidney donors were analyzed for 16 PCBs congeners and 10 OCPs. A Matrix Solid‐Phase Dispersion (MSPD) method for simultaneous determination of the target compounds in cortex homogenates was developed. A gas‐chromatography triple quadrupole mass spectrometry (Triple Quad GC‐MS) system was used for the analysis. Data were analyzed using Random Forest and Wilcoxon's rank‐sum test. OCPs and PCBs were found in specimens from both types. A subset of pollutants characterized APA more than NA. Higher concentrations (μg g−1) in APA were observed for α‐, β‐, and γ‐ Hexachlorocyclohexane (HCH) (1.48 ± 3.32 vs. 0.17 ± 0.19, P = 0.028; 2.81 ± 2.10 vs. 0.96 ± 0.98, P = 0.011; 2.16 ± 4.85 vs. 0.17 ± 0.26, P = 0.004, respectively), as well as for Hexachlorobenzene (HCB) and for PCBs 28, 52 and 101 (3.41 ± 3.11 vs. 0.97 ± 1.06, P = 0.021; 2.34 ± 4.68 vs. 0.25 ± 0.22, P = 0.039; 0.58 ± 1.19 vs. 0.06 ± 0.02, P = 0.002; 0.26 ± 0.43 vs. 0.05 ± 0.00, P = 0.001, respectively). Environmental organochlorine pollutants were shown to be present in the human normal and abnormal adrenal cortex, deserving future investigation on their possible role as adrenal endocrine disruptors in human disease. Copyright © 2017 John Wiley & Sons, Ltd.
Protective BCL11A and HBS1L‐MYB polymorphisms in a cohort of 102 Congolese patients suffering from sickle cell anemia
Authors: Mikobi Tite Minga , Tshilobo Lukusa Prosper , Aloni Michel Ntetani , Lumaka Aimé Zola , Kaba Didine Kinkodi , Devriendt Koenraad , Matthijs Gert , Mbuyi Muamba Jean Marie , Race Valérie 
Published: March 2017

BackgroundWe aimed to investigate the distribution of selected BCL11A and HMIP polymorphisms (SNP's), and to assess the correlation with HPFH in a cohort of sickle cell patients.MethodsA preliminary cross‐sectional study was conducted in 102 patients. Group 1 was composed of patients with HPFH and Group 2 consisted of patients without HbF. We assessed 8 SNPs previously associated with HPFH in cohorts genetically close to the Congolese population. Observed frequencies were compared to expected frequencies.ResultsIn the group 1, at rs7606173, the observed frequency for the genotype GG was significantly higher and the genotype GC was significantly lower than their respective expected frequencies. At rs9399137, the observed frequency of the genotype TT was significantly lower than expected. Conversely, the observed frequency of the genotype TC was significantly higher than expected. The observed frequency of the genotype TT at rs11886868 was significantly lower than the expected whereas the frequency of the genotype TC was significantly higher than observed. The lowest HbF level was recorded in patients with genotype CC at rs11886868.ConclusionIn this preliminary study, the results demonstrate that alleles of some of the 8 studied SNPs are not randomly distributed among patients with or without HPFH in this cohort.
Epithelial crypts: A complex and enigmatic olfactory organ in African and South American lungfish (Lepidosireniformes, Dipnoi)
Authors: Wittmer Carolin , Nowack Christine 
Published: March 2017

AbstractAfrican lungfish (Protopterus) seem unique among osteognathostomes in possessing a potential vomeronasal organ homolog in form of accessory epithelial crypts within their nasal cavity. Many details regarding structural and functional properties of these crypts are still unexplored. In this study, we reinvestigate the issue and also present the first data on epithelial crypts in the South American lungfish Lepidosiren paradoxa. The nasal cavities of L. paradoxa and Protopterus annectens were studied using histology, scanning electron microscopy, and alcian blue and PAS staining. In both species, the epithelial crypts consist of a pseudostratified sensory epithelium and a monolayer of elongated glandular cells, in accordance with previously published data on Protopterus. In addition, we found a new second and anatomically distinct type of mucous cell within the duct leading into the crypt. These glandular duct cells are PAS positive, whereas the elongated glandular cells are stainable with alcian blue, suggesting distinct functions of their respective secretions. Furthermore, the two lungfish species show differently structured crypt sensory epithelia and external crypt morphology, with conspicuous bilaterally symmetrical stripes of ciliated cells in L. paradoxa. Taken together, our data suggest that stimulus transport into the crypts involves both ciliary movement and odorant binding mucus.
Hen egg yolk phosvitin stimulates osteoblast differentiation in the absence of ascorbic acid
Authors: Liu Qingli , Li Chunyan , Geng Fang , Huang Xi , Ma Meihu 
Published: March 2017

AbstractBACKGROUNDEgg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3‐E1 cells by using alkaline phosphatase activity analysis, alizarin red S staining and real‐time PCR assay.RESULTSAlkaline phosphatase activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3‐E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation. Our real‐time PCR assay also indicated the two groups were similar in the expression of the osteogenic gene markers, collagen type I, osteocalcin, runt‐related transcription factor 2, and bone morphogenetic protein‐2.CONCLUSIONOur findings indicate that phosvitin plays a similar role to that of ascorbic acid in osteoblast differentiation and mineralisation. © 2017 Society of Chemical Industry
Interaction of copper nanoparticles and an endophytic growth promoter Piriformospora indica with Cajanus cajan
Authors: Rajak Jyoti , Bawaskar Manisha , Rathod Dnyaneshwar , Agarkar Gauravi , Nagaonkar Dipali , Gade Aniket , Rai Mahendra 
Published: March 2017

AbstractBACKGROUNDIn recent years, agro‐bionanotechnology has paved the way towards revolutionizing current practices in the food and agricultural fields. In the present study, the effect of copper nanoparticles (CuNPs) alone and in combination with the growth‐promoting fungus Piriformospora indica on 2‐week‐old seedlings of Cajanus cajan was evaluated. Gelatin‐coated stable CuNPs were synthesized by the chemical reduction method using hydrazine hydrate as a reducing agent.RESULTSCuNPs were characterized by using UV–visible spectroscopy, zeta potential measurement and transmission electron microscopy. The nanoparticles were found to be quite spherical with a diameter within the range 40 ± 10 nm. After the application of CuNPs and P. indica to the host plant C. cajan, the vitality of plants was determined using a Handy‐PEA (plant efficiency analyzer) instrument. Handy‐PEA analysis (which measures chlorophyll a fluorescence) indicated that seedlings inoculated with a combination of CuNPs and P. indica were the healthiest and also showed maximum vitality as compared to seedlings inoculated with CuNPs or P. indica alone.CONCLUSIONThese results suggest that CuNPs in combination with P. indica can serve as a nanobiofertilizer for enhancement of the growth and productivity of C. cajan. © 2017 Society of Chemical Industry
The impact of body mass index and nuclear β‐catenin on survival in locally advanced rectal cancer treated with preoperative radiochemotherapy
Authors: Gomez‐Millan Jaime , Queipo Maria Isabel , del Mar Delgado Maria , Perez‐Villa Lidia , Roman Alicia , De la Portilla Fernando , Torres Esperanza , De Luque Vanessa , Bayo Eloisa , Medina Jose Antonio 
Published: March 2017

Background and ObjectiveWe examined the prognostic value of obesity and nuclear β‐catenin in patients with locally advanced rectal cancer.MethodsWe prospectively recruited a total of 98 eligible patients with locally advanced cancer for preoperative radiochemotherapy followed by total mesorectal excision. Patients’ height and weight were reaorded before radiochemotherapy, and the immunohistochemical expression of nuclear β‐catenin was analyzed. Disease‐free survival (DFS) was analyzed using the Kaplan–Meier method and a Cox regression model was employed for the multivariate analysis.ResultsObese patients were associated with a lower number of recurrences (3.6% vs. 34.3%, P = 0.001), and a higher DFS (95% vs. 53%; HR, 0.09; 95%CI, 0.01–0.64; P = 0.005) than non‐obese patients. In the multivariate analysis, body mass index, nuclear β‐catenin expression, and the absence of lymph node metastases showed a significant increase in DFS.ConclusionsObesity and nuclear β‐catenin are independent favorable prognostic factors for DFS in locally advanced cancer treated with preoperative radiochemotherapy. J. Surg. Oncol. 2017;115:301–306. © 2017 Wiley Periodicals, Inc.
Discrepancy between cTNM and pTNM staging of oral cavity cancers and its prognostic significance
Authors: Choi Nayeon , Noh Yangseop , Lee Eun Kyu , Chung Manki , Baek Chung‐Hwan , Baek Kwan‐Hyuck , Jeong Han‐Sin 
Published: March 2017

IntroductionAccurate tumor‐node‐metastasis(TNM) staging of oral cavity cancer(OCC) is very important in the management of this dismal disease. However, stage migration from cTNM to pTNM was found in a portion of OCC patients. The objective of this study was to determine the possible causes of discrepancy between cTNM and pTNM in OCC and the clinical impacts of stage migration.MethodsClinical and pathological data of 252 OCC patients were retrospectively reviewed and compared each other. Clinical staging was determined through the multidisciplinary evaluation of pre‐treatment work‐ups including PET/CT. In addition, we compared the up‐staged cases with those in the no‐change group with the same pTNM stages to identify the clinical impacts of such change.ResultsClinical staging yielded overall 82.5% diagnostic accuracy in predicting pathological tumor status, and tumor extent was under‐estimated in 9.5‐13.5% of cases. The main causes of T up‐staging were under‐estimation of surface dimension (62.5%) and deep invasion to tongue extrinsic muscles (37.5%). N up‐staging was due to occult single (57.6%) and multiple (42.4%) metastases. Surprisingly, TNM up‐staging in our series did not have prognostic significance under the current management protocol.ConclusionClinical under‐estimation of pathological tumor extent occurred in approximately 13% of OCC, without clinical impacts on prognosis.
Radiofrequency ablation using a 10‐mm target margin for small hepatocellular carcinoma in patients with liver cirrhosis: A prospective randomized trial
Authors: Liao Mingheng , Zhong Xiaofei , Zhang Jingyi , Liu Yangyang , Zhu Zexin , Wu Hong , Zeng Yong , Huang Jiwei 
Published: March 2017

Background And ObjectivesTo compare 3‐year clinical outcomes of radiofrequency ablation (RFA) targeting 5‐ or 10‐mm margins for small hepatocellular carcinomas (HCCs) in cirrhotic patients.MethodsIn total, 96 cirrhotic patients with a small solitary HCC (diameter ≤3 cm) were included in this prospective trial (ChiCTRTRC‐10000954). Patients were stratified by Child‐Pugh class and randomly allocated into groups targeting either wide margins (≥10 mm, WM) or narrow margins (≥5 mm but <10 mm, NM). RFA was performed under real‐time monitoring, and ablative margins were evaluated by pre‐ and post‐operative three‐dimensional registration on CT.ResultsThe mean follow‐up time was 38.3 ± 4.8 months, 83.3% (40/48) of patients succeeded in obtaining a 10‐mm margin in WM group. Based on intention‐to‐treat analysis, the 3‐year incidences of local tumor progression (LTP) (14.9% vs 30.2%), intrahepatic recurrence (IHR) (15.0% vs 32.7%), and recurrence‐free survival (RFS) (31.7 ± 12.1 vs 24.0 ± 11.7 months) for WM group were significantly improved compared to NM group. Several prognostic factors were identified from univariate and multivariate analyses. Additionally, cirrhosis‐stratified subgroup analyses demonstrated significant survival benefits of WM in patients with Child‐Pugh class B cirrhosis.ConclusionsRFA treatment targeting 10‐mm margin may reduce the risk of tumor recurrence in cirrhotic patients with a single small HCC.
Export of terrigenous dissolved organic matter in a broad continental shelf
Authors: Medeiros Patricia M. , Babcock‐Adams Lydia , Seidel Michael , Castelao Renato M. , Di Iorio Daniela , Hollibaugh James T. , Dittmar Thorsten 
Published: March 2017

AbstractExport of terrigenous dissolved organic matter (DOM) from rivers to the ocean plays an important role in the carbon cycle. Observations from six research cruises in 2014 were used to characterize the seasonal evolution of terrigenous DOM in the shallow and broad South Atlantic Bight (SAB) shelf. While DOM with a strong terrigenous molecular, optical and isotopic signature was restricted to a coastal band early in the year, a plume with terrigenous DOM extended further to the shelf break in late spring. The offshore transport of this terrigenous DOM was consistent with wind‐driven advection in a surface Ekman layer. On time scales spanning about 1 month, the traceable riverine DOM compounds were mostly resistant to bio‐ and photo‐degradation, and the decrease in their relative abundance over the shelf following peak river discharge during spring was consistent with dilution of the river plume due to entrainment of oceanic water associated with wind‐driven mixing. Comparisons between optical absorbance measurements and ultrahigh resolution mass spectrometry data revealed that the fraction of the DOM pool with a riverine signature in the SAB can be estimated using the spectral slope coefficient of chromophoric DOM in the 275–295 nm range. This finding opens up the possibility of observing the distribution of riverine DOM on the SAB shelf in high spatial resolution and by using remote sensing methods, a crucial step for quantifying shelf‐slope exchange and the fate of terrigenous DOM in shelf seas.
Insights into the loss factors of phytoplankton blooms: The role of cell mortality in the decline of two inshore Alexandrium blooms
Authors: Choi Chang Jae , Brosnahan Michael L. , Sehein Taylor R. , Anderson Donald M. , Erdner Deana L. 
Published: March 2017

AbstractWhile considerable effort has been devoted to understanding the factors regulating the development of phytoplankton blooms, the mechanisms leading to bloom decline and termination have received less attention. Grazing and sedimentation have been invoked as the main routes for the loss of phytoplankton biomass, and more recently, viral lysis, parasitism, and programmed cell death (PCD) have been recognized as additional removal factors. Despite the importance of bloom declines to phytoplankton dynamics, the incidence and significance of various loss factors in regulating phytoplankton populations have not been widely characterized in natural blooms. To understand mechanisms controlling bloom decline, we studied two independent, inshore blooms of Alexandrium fundyense, paying special attention to cell mortality as a loss pathway. We observed increases in the number of dead cells with PCD features after the peak of both blooms, demonstrating a role for cell mortality in their terminations. In both blooms, sexual cyst formation appears to have been the dominant process leading to bloom termination, as both blooms were dominated by small‐sized gamete cells near their peaks. Cell death and parasitism became more significant as sources of cell loss several days after the onset of bloom decline. Our findings show two distinct phases of bloom decline, characterized by sexual fusion as the initial dominant cell removal processes followed by elimination of remaining cells by cell death and parasitism.