bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome and epigenome engineering CRISPR toolkit for probingin vivo cis-regulatory interactions in the chicken embryo
Authors: Williams Ruth M , Senanayake Upeka , Artibani Mara , Taylor Gunes , Wells Daniel , Ahmed Ahmed Ashour , Sauka-Spengler Tatjana 
Published: May 2017

AbstractCRISPR-Cas9 genome engineering has revolutionised all aspects of biological research, with epigenome engineering transforming gene regulation studies. Here, we present a highly efficient toolkit enabling genome and epigenome engineering in the chicken embryo, and demonstrate its utility by probing gene regulatory interactions mediated by neural crest enhancers. First, we optimise efficient guide-RNA expression from novel chick U6-mini-vectors, provide a strategy for rapid somatic gene knockout and establish protocol for evaluation of mutational penetrance by targeted next generation sequencing. We show that CRISPR/Cas9-mediated disruption of transcription factors causes a reduction in their cognate enhancer-driven reporter activity. Next, we assess endogenous enhancer function using both enhancer deletion and nuclease-deficient Cas9 (dCas9) effector fusions to modulate enhancer chromatin landscape, thus providing the first report of epigenome engineering in a developing embryo. Finally, we use the synergistic activation mediator (SAM) system to activate an endogenous target promoter. The novel genome and epigenome engineering toolkit developed here enables manipulation of endogenous gene expression and enhancer activity in chicken embryos, facilitating high-resolution analysis of gene regulatory interactionsin vivo.Summary StatementWe present an optimised toolkit for efficient genome and epigenome engineering using CRISPR in chicken embryos, with a particular focus on probing gene regulatory interactions during neural crest development.List of AbbreviationsGenome Engineering (GE), Epigenome Engineering (EGE), single guide RNA (sgRNA), Neural Crest (NC), Transcription Factor (TF), Next Generation Sequencing (NGS), somite stage (ss), Hamburger Hamilton (HH).
Live, Eat, Love: life equilibrium as a driver of organic food purchase
Authors: Husic-Mehmedovic Melika , Arslanagic-Kalajdzic Maja , Kadic-Maglajlic Selma , Vajnberger Zlatan 
Published: May 2017

Purpose
The purpose of this paper is to disentangle the effect of life equilibrium on organic food purchase intentions through a consideration of the evaluation of intrinsic and extrinsic food quality attributes. Furthermore, the study examines the role of health consciousness in achieving life equilibrium.

Design/methodology/approach
The conceptual framework was developed based on previous research and tested through a quantitative study with end consumers. The hypothesized relationships were tested using structural equation modelling.

Findings
The results obtained from this study show that the perceived quality associated with the intrinsic attributes of organic food mediates a positive influence of life equilibrium on consumers’ organic food purchase intentions. The study also confirms that life equilibrium mediates the effects of health consciousness on the evaluation of intrinsic and extrinsic food quality attributes.

Research limitations/implications
The theoretical contributions of the paper lie in uncovering the complex relationships that exist among health consciousness, life equilibrium, perceived organic food quality dimensions and purchase intentions and providing new evidence showing which perceived intrinsic organic food quality dimensions are relevant in shaping consumers’ purchase intentions.

Practical implications
The research results suggest that organic food managers should focus on developing stronger value propositions that are based more on intrinsic food quality characteristics and less on extrinsic ones.

Originality/value
This study recognizes the relevance of life equilibrium as a specific consumer lifestyle form, which drives organic food consumption through extrinsic and intrinsic food quality attributes.
Segmenting the organic food market in Lebanon: an application of k-means cluster analysis
Authors: Tleis Malak , Callieris Roberta , Roma Rocco 
Published: May 2017

Purpose
The purpose of this paper is to discover profiles of organic food consumers in Lebanon by performing a market segmentation based on lifestyle and attitude variables and thus be able to propose appropriate marketing strategies for each market segment.

Design/methodology/approach
A survey, based on the use of closed-ended questionnaire, was addressed to 320 organic food consumers in the capital Beirut, in February and March 2014. Descriptive analysis, principal component analysis and cluster analysis (k-means method) were performed upon collected data.

Findings
Four clusters were obtained and labelled based on psychographic characteristics and willingness to pay for the most purchased organic products. “Localist” and “Health conscious” clusters were the largest proportion of the selected sample, thus these were the most critical to be addressed by specific marketing strategies, emphasising the combination of local and organic food and the healthy properties of organic products. “Rational” and “Irregular” cluster were relatively small groups, addressed by pricing and promotional strategies.

Originality/value
This is the first study attempting to segment organic food consumers into different categories in a developing country as Lebanon.
Factors driving consumption behavior for green aquatic products
Authors: Li Yongtao , Zhong Changbiao 
Published: May 2017

Purpose
The purpose of this paper is to investigate the factors that influence consumers’ consumption behavior with regard to green aquatic products from a social cognitive theory perspective.

Design/methodology/approach
A web survey was used to collect the data. The survey subjects were citizens of Ningbo City in Zhejiang Province. A total of 403 subjects were obtained through the sampling service, and 337 subjects were retained after strict examination. The data were used to construct a partial least squares structural equation model.

Findings
The cognition of green aquatic products significantly positively affects outcome expectancy, self-efficacy, perception of others’ behavior, and socio-structural conditions. Self-efficacy and outcome expectancy significantly positively influence consumption intention. Self-efficacy, perception of others’ behavior, and consumption intention significantly positively affect consumption behavior.

Practical implications
The concept, connotations and benefits of green aquatic products should be widely publicized through diversified channels to increase people’s knowledge of these products and to encourage people to distinguish them from regular aquatic products and to believe in their benefits. In addition, measures should be taken to guarantee that only genuine green aquatic products can enter the market because the false claims of some products have damaged consumers’ belief in these products.

Originality/value
To test the effect of the cognition of green aquatic products, the construct “the cognition of green aquatic products” was added to the model based on social cognitive theory. This paper contributes to the existing literature by further exploring how cognitive aspects affect consumption behavior with regard to green food.
Towards successful adoption of Halal logistics and its implications for the stakeholders
Authors: Haleem Abid , Khan Mohd Imran 
Published: May 2017

Purpose
The purpose of this paper is to understand the major critical success factors (CSFs), which are instrumental for effective adoption and implementation of Halal logistics (HL) in Halal supply chain (HSC) environment.

Design/methodology/approach
In total, 15 CSFs/CSF clusters were identified and used to develop an interpretive structural modelling-based hierarchal and structural model. Further, analysis categorises driving and dependence power of factors. MICMAC has been undertaken to analyse how these CSFs and their hierarchies relate, with paths and levels.

Findings
It was found that there is a need to develop proper guidelines, standards and codes, to train the Halal logisticians. Robust ICT and its appropriate implementation seems as the backbone of the HSC. HL emerges as a key component for the Halal industry to succeed, and the same is required to extend the integrity of the Halal products from the farm to the fork. That’s to develop Halal as an intrinsic characteristic. Thus, organisations should have support from specific CSFs. The paper provides managerial implications, recommendations for effective implementation of HL and further in identifying the pull effect of HL.

Research limitations/implications
The model so developed is contextual and based on the perception of qualified experts, and they can have biasness of Halal meat supply chain.

Originality/value
An academic research taking views from different stakeholders with findings valuable to researchers and the policy planners.
Will artificial intelligence usurp white collar jobs?
Authors: Chelliah John 
Published: May 2017

Purpose
This paper highlights the risks faced by white-collar workers resulting from advances in artificial intelligence (AI).

Design/methodology/approach
This paper explores recent research and expert opinion on the evolution of AI and its encroachment on white-collar jobs.

Findings
This paper reveals susceptibility of white-collar jobs to AI.

Practical implications
This paper guides HR practitioners in advising management on the possible deployment of AI to enhance productivity and the resultant impact in the roles that employees perform.

Social implications
This study draws attention to the risks associated with the deployment of AI and as a consequence the loss of white-collar jobs.

Originality/value
This study raises the issue of how AI could disrupt the workplace by usurping white-collar jobs and creates awareness of the need for people in vulnerable white-collar jobs to re-think their careers and for HR practitioners to manage the change that this disruption will bring.
An effective approach for automatic interpretation of Chinese nominal compounds
Authors: Qu Weiguang , Dai Rubing , Wu Taizhong , Liu Jian , Zhou Junsheng , Gu Yanhui , Xu Ge 
Published: May 2017

Purpose
Automatic interpretation of Nominal Compounds is a crucial issue for many applications, for example, sentence understanding, machine translation, question-answering system and so forth. Many automatic interpretation models of Nominal Compounds use the strategies based on verbs or rules to obtain the interpretation of compounds. However, the performances of these models are still limited. The purpose of this paper is to propose an effective approach for automatic interpretation of Chinese nominal compounds.

Design/methodology/approach
The authors propose a top-down and bottom-up model based on rules and large-scale corpus for automatic interpretation of Nominal Compounds.

Findings
Experimental results demonstrate that the proposed model outperforms the state-of-the-art automatic interpretation model.

Originality/value
The paper is an up-to-date study of automatic interpretation for Nominal Compounds. It can help people understand the meaning of Nominal Compounds in reading. With a better understanding of Nominal Compounds, we can discover more hidden knowledge in them.
Public opinion dissemination on mobile internet- a case of Ebola
Authors: Wang Xiwei , Zhao Dan , Yang Mengqing , Duan Lian , Xiang Meng Meng , Guo Qiuyan 
Published: May 2017

Purpose
This study aims to improve disaster management. Social media, particularly microblog, has become a new platform for public opinion dissemination. However, few studies have been conducted to explore the structure of public opinions, the approaches for facilitating the spread of public opinions and the results of public opinion dissemination in the context of mobile internet for the purpose of improving disaster management.

Design/methodology/approach
This paper chooses Ebola as the research topic and extracts 14,735 Ebola-related data items from Sina Microblogs to examine the information nodes of public opinion and the characteristics of propagation paths on mobile internet. Particularly, nodes of public opinion between mobile terminals and non-mobile terminals are compared.

Findings
The results of this paper reveal the characteristics of public opinion propagation on mobile internet and verify the effectiveness of public opinion propagation on mobile internet. This study shows that public opinions propagate quickly, widely and efficiently and further generate great impacts on mobile internet.

Research limitations/implications
The methods used in this study can be useful for the government agencies and other relevant organizations to monitor public opinions, identify issues and problems proactively and develop strategies in a more efficient manner to improve disaster management.

Practical implications
The results of this paper are helpful for related departments to monitor public opinions and to further improve disaster management.

Originality/value
This paper explores the mechanism of public opinion dissemination on mobile internet and further investigates how to improve disaster management through a case study related to Ebola.
Public versus private land use controls in England and the USA
Authors: Walsh Emily 
Published: May 2017

Purpose
This paper aims to compare the law with regard to private property rights and restrictions and public controls in England and the USA, and the theoretical debates that surround them, to understand whether the private land use controls of nuisance and restrictive covenants could have a greater role to play or the public law system of planning is the best way to manage land.

Design/methodology/approach
This paper starts by summarising and comparing, firstly, the private laws of nuisance and restrictive covenants and then laws relating public planning, zoning and takings in England and the USA. It then reviews theoretical approaches taken in both jurisdictions to land use restrictions.

Findings
The paper concludes that private land use restrictions can only play a limited role in land management in England. Scarcity and cost of available housing necessitate a mechanism by which the state can intervene to remove or modify restrictions to enable alteration and development. The structure of freehold ownership in England and the low take-up of Commonhold as an alternative tenure mean that expansion in the use of private land use restrictions to control the use of land is unfeasible.

Originality/value
The value of this paper is that it seeks to provide insight into the contested relationship between private and public law and the relationship between property law and planning.
Deliberate concealment
Authors: Childs Sophie , Ginige Tilak A. , Pateman Hannah 
Published: May 2017

Purpose
Welwyn Hatfield Council v Secretary of State for Communities and Local Government [2009] EWHC 966 (Admin), Welwyn Hatfield Council v. Secretary of State for Communities and Local Government [2010] EWCA Civ 26 and Welwyn Hatfield Council v. Secretary of State for Communities and Local Government [2011] UKSC 15 (Beesley hereafter) and Fidler v. Secretary of State for Communities and Local Government [2010] EWHC 143 (Admin), Fidler v. Secretary of State for Communities and Local Government [2011] EWCA civ 1159 (Fidler hereafter) are two recent cases concerning deliberately concealed breaches of planning control. The defendants engaged in dishonest and misleading conduct, in an attempt to rely on a loophole within Section 171B of the Town and Country Planning Act 1990 (T&CPA). This study aims to critically analyse two solutions which were created to close the loophole; in addition, this study analyses various alternative remedies that have been suggested, and finally, whether the present law has been sufficient to remedy the situation.

Design/methodology/approach
The T&CPA is a key piece of legislation regulating planning controls; Section 171A-C provides the time limits for taking enforcement action against a breach of planning control. To achieve the above purpose, an evaluation of those provisions will be undertaken in detail. Subsequently, this study will analyse two solutions which were created to close the loophole; firstly, the Supreme Court (SC) decision (Welwyn Hatfield Borough Council v. Secretary of State for Communities and Local Government [2011] UKSC 15) and, secondly, the governments’ decision to amend the T&CPA without awaiting the SC’s decision[1].

Findings
This research concludes that the government should have awaited the SC’s decision before amending statute to prohibit reliance upon the expiration of time where there is an element of deliberate concealment. Additionally, this study suggests that the statutory amendments were not required in light of the SC’s solution in Beesley. As a result of the governments’ ill-considered decision, uncertainty has permeated through the conveyancing process, causing ambiguity, delays and additional expense in transactions at a time when a precarious property market needs anything but uncertainty.

Research limitations implications
The scope of this research is limited to deliberate concealment of breaches of planning control and the four-year enforcement period; whilst considering the consequences of the solutions proposed, this study does not provide a detailed overview of the planning system, but rather assumes prior knowledge.

Originality/value
This study offers a unique assessment of the law relating to the deliberate concealment of planning breaches and offers a thorough criticism of the law with recommendations for reform. Additionally, a variety of alternative solutions are considered. Both legal academics, planning professionals and those interested in planning law will find the paper a thought-provoking digest.
Mimebot: spherical robot visually imitating a rolling sphere
Authors: Tsuchida Shuhei , Takemori Tatsuya , Terada Tsutomu , Tsukamoto Masahiko 
Published: May 2017

Purpose
When designing a performance involving people and mobile robots, the required functions and shape of the robot must be considered. However, it can be difficult to account for all of the requirements. The purpose of this paper is to discuss a mobile robot in the shape of a ball that is used in theatrical performances.

Design/methodology/approach
The paper proposes a mobile robot that can give the audience the optical illusion of the unique movements of a sphere by mounting a spherical light-emitting diode (LED) display on a high-agility wheeled robot.

Findings
It was found that movements that are difficult to implement with existing mechanisms can nonetheless be visualized through the use of light.

Originality/value
The paper proposes the concept of using pseudo-physical movements in performances with robots. The authors built a robot that visually reproduces the movements of a rolling sphere and is capable of faster movements and easier position estimations in comparison with previous spherical robots.
Computer literacy through dance: the dancing computer project
Authors: Owen Charles B. , Dillon Laura , Dobbins Alison , Rhodes Matthew , Levinson Madeline , Keppers Noah 
Published: May 2017

Purpose
The purpose of this paper is to present the design and evolution of the Dancing Computer project. Dancing Computer is an ongoing research project at the Michigan State University, which is developing a system that aims to increase computer literacy in elementary-aged children by teaching them first to read code before they write it. The main objective is to educate children on basic concepts of computer science.

Design/methodology/approach
Children are given tablet computers that present a simple program line-by-line that they execute as they pretend to be a computer. The programs are acted out on a portable dance floor consisting of colored tiles, and the program statements instruct the child to move, turn and act out dance poses and terminology.

Findings
The Dancing Computer prototype was tested in six different locations in 2016, reaching approximately 250 students. Learning was demonstrated by significant improvements in both task duration and error performance as students performed the activities. The most common errors were movement errors, where participants failed to move the correct number of squares.

Social implications
This project has the potential to increase the level of computer literacy for thousands of children. This project’s goal is to increase understanding of what a computer does, what a program does and the step-by-step nature of computer programs.

Originality/value
This is a unique and a different approach – the norm being to start students off writing code in some language. In Dancing Computer stages children as readers of programs, allowing them to pretend to be a computer in a fun and engaging activity while also learning how computers execute real programs.
Crowdsourced real-time captioning of sign language by deaf and hard-of-hearing people
Authors: Shiraishi Yuhki , Zhang Jianwei , Wakatsuki Daisuke , Kumai Katsumi , Morishima Atsuyuki 
Published: May 2017

Purpose
The purpose of this paper is to explore the issues on how to achieve crowdsourced real-time captioning of sign language by deaf and hard-of-hearing (DHH) people, such that how a system structure should be designed, how a continuous task of sign language captioning should be divided into microtasks and how many DHH people are required to maintain a high-quality real-time captioning.

Design/methodology/approach
The authors first propose a system structure, including the new design of worker roles, task division and task assignment. Then, based on an implemented prototype, the authors analyze the necessary setting for achieving a crowdsourced real-time captioning of sign language, test the feasibility of the proposed system and explore its robustness and improvability through four experiments.

Findings
The results of Experiment 1 have revealed the optimal method for task division, the necessary minimum number of groups and the necessary minimum number of workers in a group. The results of Experiment 2 have verified the feasibility of the crowdsourced real-time captioning of sign language by DHH people. The results of Experiment 3 and Experiment 4 have shown the robustness and improvability of the captioning system.

Originality/value
Although some crowdsourcing-based systems have been developed for the captioning of voice to text, the authors intend to resolve the issues on the captioning of sign language to text, for which the existing approaches do not work well due to the unique properties of sign language. Moreover, DHH people are generally considered as the ones who receive support from others, but our proposal helps them become the ones who offer support to others.