bluuebunny/crossref_metadata_2025_split huggingface dataset:
Doing Gender, Doing Surveys? Women's Gatekeeping and Men's Non‐Participation in Multi‐Actor Reproductive Surveys
Authors: Slauson‐Blevins Kathleen , Johnson Katherine M. 
Published: June 2016

Gender differences in research participation have been documented in both quantitative and qualitative studies, a pattern that is particularly pronounced in studies of reproduction. We use the National Survey of Fertility Barriers to analyze three reasons frequently used to explain men's non‐participation in reproductive research: (1) Reproduction is a sensitive subject for men, (2) reproduction is women's domain, and (3) men's unavailability due to seemingly pragmatic reasons (e.g., work schedule). Using binary logistic regression, we test these three explanations across two different models: women's gatekeeping (denying researcher access to partners) (n = 1,637) and men's own non‐response (n = 1,454). A substantial minority of women (12.8 percent), engaged in gatekeeping, but the dominant mechanism of men's non‐participation was their own non‐response once women granted researcher access to partners. Consistent with prior studies on general survey non‐response, several demographic variables were associated with men's participation. Additionally, findings suggest that the notion of reproduction as women's domain received the strongest support, though specific variables within this construct differed for models predicting women's gatekeeping versus men's non‐response of their own accord.
Infant oral mutilation in East Africa – therapeutic and ritual grounds
Authors: Garve Roland , Garve Miriam , Link Katharina , Türp Jens C. , Meyer Christian G. 
Published: June 2016

SummaryThis paper reviews the practice and ritual traditions of infant oral mutilation, drawing on a literature search in PubMed and Google Scholar, historical reports, relevant textbooks, NGO materials and personal observations of the authors.
Remobilization of hematopoietic stem cells in healthy donors for allogeneic transplantation
Authors: Fiala Mark A. , Park Soo , Slade Michael , DiPersio John F. , Stockerl‐Goldstein Keith E. 
Published: June 2016

BACKGROUNDThe need to repeat peripheral blood (PB) stem cell mobilization and collection in healthy donors arises infrequently but may be required due to insufficient initial collection, graft failure, or relapse of the recipient's disease. Little data exist on the efficacy of remobilization. Therefore, we retrospectively reviewed 18 years of remobilization records from healthy stem cell donors at our institution.STUDY DESIGN AND METHODSWe identified 62 healthy donors who underwent remobilization, a cohort of 30 mobilized and remobilized with cytokines and a cohort of 32 mobilized with a CXCR4 antagonist and remobilized with cytokines. For each cohort we compared the PB CD34+/L level, the number of CD34+ cells collected/kg (recipient weight), and the number of CD34+ cells/L collected on the first day of leukapheresis during initial mobilization and remobilization.RESULTSInitial mobilization with cytokines was associated with reduced remobilization. The mean PB CD34/L level at initial mobilization was 69 × 106 compared to 37 × 106 at remobilization (p = 0.029). In contrast, initial mobilization with a CXCR4 antagonist was not associated with reduced remobilization. The mean PB CD34/L level at initial mobilization was 15 × 106 compared to 68 × 106 at remobilization (p < 0.001). In both cohorts, initial mobilization results were positively correlated with remobilization results but the interval between was not.CONCLUSIONSThis study suggests that poor remobilization yields may be due to decreased efficacy of cytokines after repeat exposure. The underlying mechanism of these findings remains unclear and further studies are needed.
Formation of mitochondrial‐derived vesicles is an active and physiologically relevant mitochondrial quality control process in the cardiac system
Authors: Cadete Virgilio J. J. , Deschênes Sonia , Cuillerier Alexanne , Brisebois François , Sugiura Ayumu , Vincent Amy , Turnbull Doug , Picard Martin , McBride Heidi M. , Burelle Yan 
Published: June 2016

Key points
Mitochondrial‐derived vesicle (MDV) formation occurs under baseline conditions and is rapidly upregulated in response to stress‐inducing conditions in H9c2 cardiac myoblasts.
In mice formation of MDVs occurs readily in the heart under normal healthy conditions while mitophagy is comparatively less prevalent.
In response to acute stress induced by doxorubicin, mitochondrial dysfunction develops in the heart, triggering MDV formation and mitophagy.
MDV formation is thus active in the cardiac system, where it probably constitutes a baseline housekeeping mechanism and a first line of defence against stress.
AbstractThe formation of mitochondrial‐derived vesicles (MDVs), a process inherited from bacteria, has emerged as a potentially important mitochondrial quality control (QC) mechanism to selectively deliver damaged material to lysosomes for degradation. However, the existence of this mechanism in various cell types, and its physiological relevance, remains unknown. Our aim was to investigate the dynamics of MDV formation in the cardiac system in vitro and in vivo. Immunofluorescence in cell culture, quantitative transmission electron microscopy and electron tomography in vivo were used to study MDV production in the cardiac system. We show that in cardiac cells MDV production occurs at baseline, is commensurate with the dependence of cells on oxidative metabolism, is more frequent than mitophagy and is up‐regulated on the time scale of minutes to hours in response to prototypical mitochondrial stressors (antimycin‐A, xanthine/xanthine oxidase). We further show that MDV production is up‐regulated together with mitophagy in response to doxorubicin‐induced mitochondrial and cardiac dysfunction. Here we provide the first quantitative data demonstrating that MDV formation is a mitochondrial QC operating in the heart.
The Indoor Fungus Cladosporium halotolerans Survives Humidity Dynamics Markedly Better than Aspergillus niger and Penicillium rubens despite Less Growth at Lowered Steady-State Water Activity
Authors: Segers Frank J. J. , van Laarhoven Karel A. , Huinink Hendrik P. , Adan Olaf C. G. , Wösten Han A. B. , Dijksterhuis Jan 
Published: June 2016

ABSTRACT

Indoor fungi cause damage in houses and are a potential threat to human health. Indoor fungal growth requires water, for which the terms water activity (a
w
) and relative humidity (RH) are used. The ability of the fungi
Aspergillus niger
,
Cladosporium halotolerans
, and
Penicillium rubens
at different developmental stages to survive changes in a
w
dynamics was studied. Fungi grown on media with high a
w
were transferred to a controlled environment with low RH and incubated for 1 week. Growth of all developmental stages was halted during incubation at RHs below 75%, while growth continued at 84% RH. Swollen conidia, germlings, and microcolonies of
A. niger
and
P. rubens
could not reinitiate growth when retransferred from an RH below 75% to a medium with high a
w
. All developmental stages of
C. halotolerans
showed growth after retransfer from 75% RH. Dormant conidia survived retransfer to medium with high a
w
in all cases. In addition, retransfer from 84% RH to medium with high a
w
resulted in burst hyphal tips for
Aspergillus
and
Penicillium
. Cell damage of hyphae of these fungi after incubation at 75% RH was already visible after 2 h, as observed by staining with the fluorescent dye TOTO-1. Thus,
C. halotolerans
is more resistant to a
w
dynamics than
A. niger
and
P. rubens
, despite its limited growth compared to that of these fungi at a lowered steady-state a
w
. The survival strategy of this phylloplane fungus in response to the dynamics of a
w
is discussed in relation to its morphology as studied by cryo-scanning electron microscopy (cryo-SEM).

IMPORTANCE
Indoor fungi cause structural and cosmetic damage in houses and are a potential threat to human health. Growth depends on water, which is available only at certain periods of the day (e.g., during cooking or showering). Knowing why fungi can or cannot survive indoors is important for finding novel ways of prevention. Until now, the ability of fungi to grow on media with little available water at steady state (unchanging conditions) has been important for evaluating whether a fungus can grow indoors. In the present study, we found that the fungus
Cladosporium halotolerans
, a common indoor fungus, is more resistant to changes in available water than the fungi
Aspergillus niger
and
Penicillium rubens
, despite the fact that the latter fungi can grow on media with low water availability. We concluded that the ability of fungi to deal with changes in humidity is at least as important as the ability to grow on low-water media.
Biogeographical Patterns of Legume-Nodulating Burkholderia spp.: from African Fynbos to Continental Scales
Authors: Lemaire Benny , Chimphango Samson B. M. , Stirton Charles , Rafudeen Suhail , Honnay Olivier , Smets Erik , Chen Wen-Ming , Sprent Janet , James Euan K. , Muasya A. Muthama 
Published: June 2016

ABSTRACTRhizobia of the genusBurkholderiahave large-scale distribution ranges and are usually associated with South African papilionoid and South American mimosoid legumes, yet little is known about their genetic structuring at either local or global geographic scales. To understand variation at different spatial scales, from individual legumes in the fynbos (South Africa) to a global context, we analyzed chromosomal (16S rRNA,recA) and symbiosis (nifH,nodA,nodC) gene sequences. We showed that the global diversity of nodulation genes is generally grouped according to the South African papilionoid or South American mimosoid subfamilies, whereas chromosomal sequence data were unrelated to biogeography. While nodulation genes are structured on a continental scale, a geographic or host-specific distribution pattern was not detected in the fynbos region. In host range experiments, symbiotic promiscuity ofBurkholderiatuberumSTM678TandB.phymatumSTM815Twas discovered in selected fynbos species. Finally, a greenhouse experiment was undertaken to assess the ability of mimosoid (Mimosapudica) and papilionoid (Dipogonlignosus,Indigoferafilifolia,Macroptiliumatropurpureum, andPodalyriacalyptrata) species to nodulate in South African (fynbos) and Malawian (savanna) soils. While theBurkholderia-philous fynbos legumes (D.lignosus,I.filifolia, andP.calyptrata) nodulated only in their native soils, the invasive neotropical speciesM.pudicadid not develop nodules in the African soils. The fynbos soil, notably rich inBurkholderia, seems to retain nodulation genes compatible with the local papilionoid legume flora but is incapable of nodulating mimosoid legumes that have their center of diversity in South America.IMPORTANCEThis study is the most comprehensive phylogenetic assessment of root-nodulatingBurkholderiaand investigated biogeographic and host-related patterns of the legume-rhizobial symbiosis in the South African fynbos biome, as well as at global scales, including native species from the South American Caatinga and Cerrado biomes. While a global investigation of the rhizobial diversity revealed distinct nodulation and nitrogen fixation genes among South African and South American legumes, regionally distributed species in the Cape region were unrelated to geographic and host factors.
Shotgun Metagenomic Profiles Have a High Capacity To Discriminate Samples of Activated Sludge According to Wastewater Type
Authors: Ibarbalz Federico M. , Orellana Esteban , Figuerola Eva L. M. , Erijman Leonardo 
Published: June 2016

ABSTRACT
This study was conducted to investigate whether functions encoded in the metagenome could improve our ability to understand the link between microbial community structures and functions in activated sludge. By analyzing data sets from six industrial and six municipal wastewater treatment plants (WWTPs), covering different configurations, operational conditions, and geographic regions, we found that wastewater influent composition was an overriding factor shaping the metagenomic composition of the activated sludge samples. Community GC content profiles were conserved within treatment plants on a time scale of years and between treatment plants with similar influent wastewater types. Interestingly, GC contents of the represented phyla covaried with the average GC contents of the corresponding WWTP metagenome. This suggests that the factors influencing nucleotide composition act similarly across taxa and thus the variation in nucleotide contents is driven by environmental differences between WWTPs. While taxonomic richness and functional richness were correlated, shotgun metagenomics complemented taxon-based analyses in the task of classifying microbial communities involved in wastewater treatment systems. The observed taxonomic dissimilarity between full-scale WWTPs receiving influent types with varied compositions, as well as the inferred taxonomic and functional assignment of recovered genomes from each metagenome, were consistent with underlying differences in the abundance of distinctive sets of functional categories. These conclusions were robust with respect to plant configuration, operational and environmental conditions, and even differences in laboratory protocols.

IMPORTANCE
This work contributes to the elucidation of drivers of microbial community assembly in wastewater treatment systems. Our results are significant because they provide clear evidence that bacterial communities in WWTPs assemble mainly according to influent wastewater characteristics. Differences in bacterial community structures between WWTPs were consistent with differences in the abundance of distinctive sets of functional categories, which were related to the metabolic potential that would be expected according to the source of the wastewater.
Draft Genome Sequences of Vancomycin-Intermediate Staphylococcus aureus Strains in South Korea
Authors: Kim Jung Wook , Yoo Jae Il , Kang Gi Su , Lee Yeong Seon , Yu Jae-Yon , Park Chan , Kim Il-Hwan 
Published: June 2016

ABSTRACT

We report here the draft genome sequences of four vancomycin-intermediate
Staphylococcus aureus
(VISA) strains from South Korean hospitals participating in a nationwide laboratory surveillance program for vancomycin-intermediate and vancomycin-resistant
Staphylococcus aureus
. All strains harbor mutations in the
walKR
,
graSR
, and/or
rpoB
genes that are known frequently mutated determinants of VISA.
Genome Sequence of Lactobacillus johnsonii Strain W1, Isolated from Mice
Authors: Wu Xiaolin , Zhao Chunyan , Guo Zhonghe , Hao Yuchong , Li Jinghua , Shi Hongyan , Sun Yanbo 
Published: June 2016

ABSTRACT

Lactobacillus johnsonii
, a member of the gut lactobacilli, plays an important role in normal gut functioning. Here, we report the draft genome sequence of
L. johnsonii
strain W1 isolated from ICR mice.
Draft Genome Sequence of Bacillus farraginis R-6540
T
(DSM 16013), a Spore-Forming Bacterium Isolated at Dairy Farms
Authors: Wang Jie-ping , Liu Bo , Liu Guo-hong , Ge Ci-bin , Xiao Rong-feng , Zheng Xue-fang , Shi Huai 
Published: June 2016

ABSTRACT

Bacillus farraginis
R-6540
T
is a Gram-positive, aerobic, and spore-forming bacterium with very high intrinsic heat resistance. Here, we report the 5.32-Mb draft genome sequence of
B. farraginis
R-6540
T
, which is the first genome sequence of this species and will promote its fundamental research.
Complete Genome Sequences of Four Strains from the 2015-2016 Elizabethkingia anophelis Outbreak
Authors: Nicholson Ainsley C. , Whitney Anne M. , Emery Brian D. , Bell Melissa E. , Gartin Jarrett T. , Humrighouse Ben W. , Loparev Vladimir N. , Batra Dhwani , Sheth Mili , Rowe Lori A. , Juieng Phalasy , Knipe Kristen , Gulvik Christopher , McQuiston John R. 
Published: June 2016

ABSTRACT

The complete circularized genome sequences of selected specimens from the largest known
Elizabethkingia anophelis
outbreak to date are described here. Genomic rearrangements observed among the outbreak strains are discussed.
Draft Genome Sequences of
Kosmotoga
sp. Strain DU53 and Kosmotoga arenicorallina S304
Authors: Pollo Stephen M. J. , Charchuk Rhianna , Nesbø Camilla L. 
Published: June 2016

ABSTRACT

Here, we announce the draft genome sequences of two thermophilic
Thermotogae
bacteria:
Kosmotoga
sp. strain DU53, isolated from a continental oil reservoir, and
Kosmotoga arenicorallina
, isolated from hydrothermal sediments. The sequences will provide further insight into evolution of the
Kosmotogales
.
Draft Genome Sequence of
Chryseobacterium
sp. Strain GSE06, a Biocontrol Endophytic Bacterium Isolated from Cucumber (
Cucumis sativus
)
Authors: Jeong Jin-Ju , Park Byeong Hyeok , Park Hongjae , Choi In-Geol , Kim Ki Deok 
Published: June 2016

ABSTRACT

Chryseobacterium
sp. strain GSE06 is a biocontrol endophytic bacterium against the destructive soilborne oomycete
Phytophthora capsici,
which causes Phytophthora blight of pepper. Here, we present its draft genome sequence, which contains genes related to biocontrol traits, such as colonization, antimicrobial activity, plant growth promotion, and abiotic or biotic stress adaptation.
Genome Sequences of Pseudomonas oryzihabitans Phage POR1 and Pseudomonas aeruginosa Phage PAE1
Authors: Dyson Zoe A. , Seviour Robert J. , Tucci Joseph , Petrovski Steve 
Published: June 2016

ABSTRACT

We report the genome sequences of two double-stranded DNA siphoviruses, POR1 infective for
Pseudomonas oryzihabitans
and PAE1 infective for
Pseudomonas aeruginosa
. The phage POR1 genome showed no nucleotide sequence homology to any other DNA phage sequence in the GenBank database, while phage PAE1 displayed synteny to
P. aeruginosa
phages M6, MP1412, and YuA.
Preventing overuse shoulder injuries among throwing athletes: a cluster-randomised controlled trial in 660 elite handball players
Authors: Andersson Stig Haugsboe , Bahr Roald , Clarsen Benjamin , Myklebust Grethe 
Published: June 2016

BackgroundShoulder problems are highly prevalent among elite handball players. Reduced glenohumeral rotation, external rotation weakness and scapula dyskinesis have been identified as risk factors.AimEvaluate the effect of an exercise programme designed to reduce the prevalence of shoulder problems in elite handball.Methods45 elite handball teams (22 female teams, 23 male teams, 660 players) were cluster randomised (22 teams, 331 players in the intervention group, 23 teams, 329 players in the control group) and followed for 1 competitive season (7 months). The Oslo Sports Trauma Research Center (OSTRC) Shoulder Injury Prevention Programme, an exercise programme to increase glenohumeral internal rotation, external rotation strength and scapular muscle strength, as well as improve kinetic chain and thoracic mobility, was delivered by coaches and captains 3 times per week as a part of the handball warm-up. The main outcome measures, prevalence of shoulder problems and substantial shoulder problems, were measured monthly.ResultsThe average prevalence of shoulder problems during the season was 17% (95% CI 16% to 19%) in the intervention group and 23% (95% CI 21% to 26%) in the control group (mean difference 6%). The average prevalence of substantial shoulder problems was 5% (95% CI 4% to 6%) in the intervention group and 8% (95% CI 7% to 9%) in the control group (mean difference 3%). Using generalised estimating equation models, a 28% lower risk of shoulder problems (OR 0.72, 95% CI 0.52 to 0.98, p=0.038) and 22% lower risk of substantial shoulder problems (OR 0.78, 95% CI 0.53 to 1.16, p=0.23) were observed in the intervention group compared with the control group.ConclusionsThe OSTRC Shoulder Injury Prevention Programme reduced the prevalence of shoulder problems in elite handball and should be included as a part of the warm-up.Trial registration numberISRCTN96217107.
Fetal umbilical artery Doppler pulsatility index and childhood neurocognitive outcome at 12 years
Authors: Mone Fionnuala , McConnell Barbara , Thompson Andrew , Segurado Ricardo , Hepper Peter , Stewart Moira C , Dornan James C , Ong Stephen , McAuliffe Fionnuala M , Shields Michael D 
Published: June 2016

ObjectiveTo determine whether an elevated fetal umbilical artery Doppler (UAD) pulsatility index (PI) at 28 weeks’ gestation, in the absence of fetal growth restriction (FGR) and prematurity, is associated with adverse neurocognitive outcome in children aged 12 years.MethodsProspective cohort study, comparing children with a normal fetal UAD PI (<90th centile) (n=110) and those with an elevated PI (≥90th centile) (n=40). UAD was performed at 28, 32 and 34 weeks gestation. At 12 years of age, all children were assessed under standardised conditions at Queen's University, Belfast, UK to determine cognitive and behavioural outcomes using the British Ability Score-II and Achenbach Child Behavioural Checklist Parent Rated Version under standardised conditions. Regression analysis was performed, controlling for confounders such as gender, socioeconomic status and age at assessment.ResultsThe mean age of follow-up was 12.4 years (±0.5 SD) with 44% of children male (n=63). When UAD was assessed at 28 weeks, the elevated fetal UAD group had lower scores in cognitive assessments of information processing and memory. Parameters included (1) recall of objects immediate verbal (p=0.002), (2) delayed verbal (p=0.008) and (3) recall of objects immediate spatial (p=0.0016). There were no significant differences between the Doppler groups at 32 or 34 weeks' gestation.ConclusionsAn elevated UAD PI at 28 weeks' gestation in the absence of FGR or prematurity is associated with lower scores of declarative memory in children aged 12 years. A potential explanation for this is an element of placental insufficiency in the presence of the appropriately grown fetus, which affects the development of the fetal hippocampus and information processing and memory long-term. These findings, however, had no impact on overall academic ability, mental processing and reasoning or overall behavioural function.
Tai Chi for stroke rehabilitation: protocol for a systematic review
Authors: Zhang Yong , Wang Shanshan , Chen Pei , Zhu Xiaoshu , Li Zongheng 
Published: June 2016

IntroductionStroke is a major cause of death and disability, and imposes a huge burden and significant workload for patients, their families and society. As a special form of physical activity, Tai Chi is may be useful for stroke rehabilitation. The objective of this review is to systematically evaluate the efficacy and safety of Tai Chi for rehabilitation in stroke patients.Methods and analysisWe will conduct a systematic search of the following electronic databases from their inception to 31 October 2015: MEDLINE, EMBASE, the Cochrane Library, the Chinese BioMedical Literature Database (CBM), the Chinese National Knowledge Infrastructure (CNKI), the Chinese Science and Technology Periodical Database (VIP), Wanfang and the Chinese Dissertation Database. All relevant randomised controlled trials (RCTs) in English and Chinese will be included. The main outcomes will be changes in the neurological function of patients and in independence in activities of daily living. Adverse events, adherence, costs and the cost effectiveness of Tai Chi will also be assessed. Two independent reviewers will select studies, extract data and assess quality. Review Manager 5.3 will be used for assessment of risk of bias, data synthesis and subgroup analysis.Ethics and disseminationThis systematic review does not require formal ethical approval because all data will be analysed anonymously. Results will provide a general overview and evidence concerning the efficacy and safety of Tai Chi for stroke rehabilitation. Findings will be disseminated through peer-reviewed publications.Trial registration numberCRD42015026999.