bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct intracellular signaling mediates C‐MET regulation of dendritic growth and synaptogenesis
Authors: Eagleson Kathie L. , Lane Christianne J. , McFadyen‐Ketchum Lisa , Solak Sara , Wu Hsiao‐Huei , Levitt Pat 
Published: January 2016

ABSTRACTHepatocyte growth factor (HGF) activation of the MET receptor tyrosine kinase influences multiple neurodevelopmental processes. Evidence from human imaging and mouse models shows that, in the forebrain, disruptions in MET signaling alter circuit formation and function. One likely means of modulation is by controlling neuron maturation. Here, we examined the signaling mechanisms through which MET exerts developmental effects in the neocortex. In situ hybridization revealed that hgf is located near MET‐expressing neurons, including deep neocortical layers and periventricular zones. Western blot analyses of neocortical crude membranes demonstrated that HGF‐induced MET autophosphorylation peaks during synaptogenesis, with a striking reduction in activation between P14 and P17 just before pruning. In vitro analysis of postnatal neocortical neurons assessed the roles of intracellular signaling following MET activation. There is rapid, HGF‐induced phosphorylation of MET, ERK1/2, and Akt that is accompanied by two major morphological changes: increases in total dendritic growth and synapse density. Selective inhibition of each signaling pathway altered only one of the two distinct events. MAPK/ERK pathway inhibition significantly reduced the HGF‐induced increase in dendritic length, but had no effect on synapse density. In contrast, inhibition of the PI3K/Akt pathway reduced HGF‐induced increases in synapse density, with no effect on dendritic length. The data reveal a key role for MET activation during the period of neocortical neuron growth and synaptogenesis, with distinct biological outcomes mediated via discrete MET‐linked intracellular signaling pathways in the same neurons. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1160–1181, 2016
Iberian red deer: paraphyletic nature at mtDNAbut nuclear markers support its genetic identity
Authors: Carranza Juan , Salinas María , de Andrés Damián , Pérez‐González Javier 
Published: January 2016

AbstractRed deer populations in the Iberian glacial refugium were the main source for postglacial recolonization and subspecific radiation in north‐western Europe. However, the phylogenetic history of Iberian red deer(Cervus elaphus hispanicus)and its relationships with northern European populations remain uncertain. Here, we studyDNAsequences at the mitochondrial control region along withSTRmarkers for over 680 specimens from all the main red deer populations in Spain and other west European areas. Our results from mitochondrial and genomicDNAshow contrasting patterns, likely related to the nature of these types ofDNAmarkers and their specific processes of change over time. The results, taken together, bring support to two distinct, cryptic maternal lineages for Iberian red deer that predated the last glacial maximum and that have maintained geographically well differentiated until present. Haplotype relationships show that only one of them contributed to the northern postglacial recolonization. However, allele frequencies of nuclear markers evidenced one main differentiation between Iberian and northern European subspecies although also supported the structure of both matrilines within Iberia. Thus, our findings reveal a paraphyletic nature for Iberian red deer but also its genetic identity and differentiation with respect to northern subspecies. Finally, we suggest that maintaining the singularity of Iberian red deer requires preventing not only restocking practices with red deer specimens belonging to other European populations but also translocations between both Iberian lineages.
Hybridization in the Cetacea: widespread occurrence and associated morphological, behavioral, and ecological factors
Authors: Crossman Carla A. , Taylor Eric B. , Barrett‐Lennard Lance G. 
Published: January 2016

AbstractHybridization has been documented in a many different pairs of cetacean species both in captivity and in the wild. The widespread occurrence of hybridization indicates that postmating barriers to interbreeding are incomplete within the order Cetacea, and therefore raises questions about how species integrity is maintained in the face of interspecific (and often intergeneric) gene flow. We examined hybridization across the order Cetacea (oceanic species included: N = 78; species with 44 chromosomes included: N = 52) to test for associations between the occurrence of hybridization and similarity across 13 ecological, morphological and behavioral traits in hybridizing vs. non‐hybridizing species pairs. We found that species pairs that share a greater number of traits had a higher propensity to hybridize than pairs of species that did not. This trend was driven by behavioral and morphological traits such as vocalization frequency and body size. Together our findings suggest the importance of divergent selection on morphological and behavioral traits within sympatric species in constraining opportunities for hybridization and preventing the collapse of parental species.
Urban gardens promote bee foraging over natural habitats and plantations
Authors: Kaluza Benjamin F. , Wallace Helen , Heard Tim A. , Klein Alexandra‐Maria , Leonhardt Sara D. 
Published: January 2016

AbstractIncreasing human land use for agriculture and housing leads to the loss of natural habitat and to widespread declines in wild bees. Bee foraging dynamics and fitness depend on the availability of resources in the surrounding landscape, but how precisely landscape related resource differences affect bee foraging patterns remains unclear. To investigate how landscape and its interaction with season and weather drive foraging and resource intake in social bees, we experimentally compared foraging activity, the allocation of foragers to different resources (pollen, nectar, and resin) and overall resource intake in the Australian stingless bee Tetragonula carbonaria (Apidae, Meliponini). Bee colonies were monitored in different seasons over two years. We compared foraging patterns and resource intake between the bees' natural habitat (forests) and two landscapes differently altered by humans (suburban gardens and agricultural macadamia plantations). We found foraging activity as well as pollen and nectar forager numbers to be highest in suburban gardens, intermediate in forests and low in plantations. Foraging patterns further differed between seasons, but seasonal variations strongly differed between landscapes. Sugar and pollen intake was low in plantations, but contrary with our predictions, it was even higher in gardens than in forests. In contrast, resin intake was similar across landscapes. Consequently, differences in resource availability between natural and altered landscapes strongly affect foraging patterns and thus resource intake in social bees. While agricultural monocultures largely reduce foraging success, suburban gardens can increase resource intake well above rates found in natural habitats of bees, indicating that human activities can both decrease and increase the availability of resources in a landscape and thus reduce or enhance bee fitness.
Effect of telemonitoring of cardiac implantable electronic devices on healthcare utilization: a meta‐analysis of randomized controlled trials in patients with heart failure
Authors: Klersy Catherine , Boriani Giuseppe , De Silvestri Annalisa , Mairesse Georges H. , Braunschweig Frieder , Scotti Valeria , Balduini Anna , Cowie Martin R. , Leyva Francisco ,   for the Health Economics Committee of the European Heart Rhythm Association
Published: January 2016

AimsImplantable device telemonitoring (DTM) is a diagnostic adjunct to traditional face‐to‐face hospital visits. Remote device follow‐up and earlier diagnoses facilitated by DTM should reduce healthcare utilization. We explored whether DTM reduces healthcare utilization over standard of care (SoC), without compromising patient outcomes.Methods and resultsThis systematic review and meta‐analysis of 11 randomized controlled trials on DTM in patients with heart failure consisted of 5702 patients, with a median of 117 [interquartile range (IQR) 76–331] patients per study [age 65 years (IQR 63–67)] and follow‐up range of 12–36 months. DTM was associated with a reduction in total number of visits [planned, unplanned, and emergency room (ER)] [relative risk (RR) 0.56; 95% confidence interval (CI) 0.43–0.73, P < 0.001]. Rates of cardiac hospitalizations (RR 0.96; 95% CI 0.82–1.12, P = 0.60) and the composite endpoints of ER, unplanned hospital visits, or hospitalizations (RR 0.99; 95% CI 0.68–1.43, P = 0.96) was similar between the DTM and the SoC groups. An increase in the total number of ER or unscheduled visits (RR 1.37; 95% CI 1.11–1.70, P = 0.004) was observed. This effect was consistent and statistically significant for all studies. Total and cardiac mortality were similar between the groups (DTM RR 0.90; 95% CI 0.69–1.16, P = 0.41; and DTM RR 0.93; 95% CI 0.51–1.69, P = 0.80). Monetary costs favoured DTM (10–55% reduction in five studies).ConclusionsCompared with SoC, DTM is associated with a marked reduction in planned hospital visits. In addition, DTM was associated with lower monetary costs, despite a modest increase in unplanned hospital and ER visits. DTM did not compromise survival.
The interaction of perceived control and Gambler's fallacy in risky decision making: An fMRI study
Authors: Shao Robin , Sun Delin , Lee Tatia M.C. 
Published: January 2016

AbstractLimited recent evidence implicates the anterior/posterior cingulate (ACC/PCC) and lateral prefrontal networks as the neural substrates of risky decision‐making biases such as illusions of control (IoC) and gambler's fallacy (GF). However, investigation is lacking on the dynamic interactive effect of those biases during decision making. Employing a card‐guessing game that independently manipulates trial‐by‐trial perceived control and gamble outcome among 29 healthy female participants, we observed both IoC‐ and GF‐type behaviors, as well as an interactive effect of previous control and previous outcome, with GF‐type behaviors only following computer‐selected, but not self‐selected, outcomes. Imaging results implicated the ACC and left dorsolateral prefrontal cortex (DLPFC) in agency processing, and the cerebellum and right DLPFC in previous outcome processing, in accordance with past literature. Critically, the right inferior parietal lobule (IPL) exhibited significant betting‐related activities to the interaction of previous control and previous outcome, showing more positive signals to previous computer‐selected winning versus losing outcomes but the reverse pattern following self‐selected outcomes, as well as responding to the interactive effect of control and outcome during feedback. Associations were also found between participants' behavioral sensitivity to the interactive effect of previous control and previous outcome, and right IPL signals, as well as its functional connectivity with neural networks implicated in agency and previous outcome processing. We propose that the right IPL provides the neural substrate for the interaction of perceived control and GF, through coordinating activities in the anterior and posterior cingulate cortices and working conjunctively with lateral PFC and other parietal networks. Hum Brain Mapp 37:1218–1234, 2016. © 2016 Wiley Periodicals, Inc.
Runoff initiation in a preserved semiarid Caatinga small watershed, Northeastern Brazil
Authors: de Figueiredo José Vidal , de Araújo José Carlos , Medeiros Pedro Henrique Augusto , Costa Alexandre C. 
Published: January 2016

AbstractThis study analyses some hydrological driving forces and their interrelation with surface‐flow initiation in a semiarid Caatinga basin (12 km2), Northeastern Brazil. During the analysis period (2005 – 2014), 118 events with precipitation higher than 10 mm were monitored, providing 45 events with runoff, 25 with negligible runoff and 49 without runoff. To verify the dominant processes, 179 on‐site measurements of saturated hydraulic conductivity (Ksat) were conducted. The results showed that annual runoff coefficient lay below 0.5% and discharge at the outlet has only occurred four days per annum on average, providing an insight to the surface‐water scarcity of the Caatinga biome. The most relevant variables to explain runoff initiation were total precipitation and maximum 60‐min rainfall intensity (I60). Runoff always occurred when rainfall surpassed 31 mm, but it never occurred for rainfall below 14 mm or for I60 below 12 mm h−1. The fact that the duration of the critical intensity is similar to the basin concentration time (65 min) and that the infiltration threshold value approaches the river‐bank saturated hydraulic conductivity support the assumption that Hortonian runoff prevails. However, none of the analysed variables (total or precedent precipitation, soil moisture content, rainfall intensities or rainfall duration) has been able to explain the runoff initiation in all monitored events: the best criteria, e.g. failed to explain 27% of the events. It is possible that surface‐flow initiation in the Caatinga biome is strongly influenced by the root‐system dynamics, which changes macro‐porosity status and, therefore, initial abstraction. Copyright © 2016 John Wiley & Sons, Ltd.
Could operational hydrological models be made compatible with satellite soil moisture observations?
Authors: Zhuo Lu , Han Dawei 
Published: January 2016

AbstractSoil moisture is a significant state variable in flood forecasting. Nowadays more and more satellite soil moisture products are available, yet their usage in the operational hydrology is still limited. This is because the soil moisture state variables in most operational hydrological models (mostly conceptual models) are over‐simplified—resulting in poor compatibility with the satellite soil moisture observations. A case study is provided to discuss this in more detail, with the adoption of the XAJ model and the Soil Moisture and Ocean Salinity (SMOS) level‐3 soil moisture observation to illustrate the relevant issues. It is found that there are three distinct deficiencies existed in the XAJ model that could cause the mismatch issues with the SMOS soil moisture observation: (i) it is based on runoff generation via the field capacity excess mechanism (interestingly, such a runoff mechanism is called the saturation excess in XAJ while in fact it is clearly a misnomer); (ii) evaporation occurs at the potential rate in its upper soil layer until the water storage in the upper layer is exhausted, and then the evapotranspiration process from the lower layers will commence – leading to an abrupt soil water depletion in the upper soil layer; (iii) it uses the multi‐bucket concept at each soil layer – hence the model has varied soil layers. Therefore, it is a huge challenge to make an operational hydrological model compatible with the satellite soil moisture data. The paper argues that this is possible and some new ideas have been explored and discussed. Copyright © 2016 John Wiley & Sons, Ltd.
Large seasonal changes in the recharge of seawater in a subterranean estuary revealed by a radon tracer
Authors: Oh Yong Hwa , Kim Guebuem 
Published: January 2016

AbstractActivities of radon (222Rn) in groundwater were continuously monitored in a saline aquifer from September 2010 to July 2011. The activities of 222Rn ranged from 200 to 4300 Bq m−3, with a large seasonal variation. Because the activity of 222Rn in seawater is low, 222Rn in saline groundwater must be produced in the aquifer from radium (226Ra) in rocks and sediments. The 222Rn activities were higher in the warm‐dry seasons (September–November 2010 and April–May 2011) when the saline aquifer was stable. In contrast, the lowest 222Rn activities were observed in the cold‐dry season (December 2010 and January–March 2011), because of the effective exchange between groundwater and seawater. In addition, sudden decreases of 222Rn activities coincided with episodic drops in groundwater temperatures. These results reveal that lower seawater temperature in winter may result in density‐driven seawater intrusion. During the wet season (June–July 2011), 222Rn activities were more clearly affected by semi‐monthly and diurnal tidal pumping, showing higher 222Rn activities during low and spring tides. Such a tidal effect was not clearly observed during the warm‐dry and cold‐dry seasons. This result implies that the residence time of SGD in coastal zones is significantly affected by seasonal changes in driving forces such as tidal pumping and seawater intrusion. Copyright © 2016 John Wiley & Sons, Ltd.
The responses of immune cells to iron oxide nanoparticles
Authors: Xu Yaolin , Sherwood Jennifer A. , Lackey Kimberly H. , Qin Ying , Bao Yuping 
Published: January 2016

AbstractImmune cells play an important role in recognizing and removing foreign objects, such as nanoparticles. Among various parameters, surface coatings of nanoparticles are the first contact with biological system, which critically affect nanoparticle interactions. Here, surface coating effects on nanoparticle cellular uptake, toxicity and ability to trigger immune response were evaluated on a human monocyte cell line using iron oxide nanoparticles. The cells were treated with nanoparticles of three types of coatings (negatively charged polyacrylic acid, positively charged polyethylenimine and neutral polyethylene glycol). The cells were treated at various nanoparticle concentrations (5, 10, 20, 30, 50 μg ml−1 or 2, 4, 8, 12, 20 μg cm−2) with 6 h incubation or treated at a nanoparticle concentration of 50 μg ml−1 (20 μg cm−2) at different incubation times (6, 12, 24, 48 or 72 h). Cell viability over 80% was observed for all nanoparticle treatment experiments, regardless of surface coatings, nanoparticle concentrations and incubation times. The much lower cell viability for cells treated with free ligands (e.g. ~10% for polyethylenimine) suggested that the surface coatings were tightly attached to the nanoparticle surfaces. The immune responses of cells to nanoparticles were evaluated by quantifying the expression of toll‐like receptor 2 and tumor necrosis factor‐α. The expression of tumor necrosis factor‐α and toll‐like receptor 2 were not significant in any case of the surface coatings, nanoparticle concentrations and incubation times. These results provide useful information to select nanoparticle surface coatings for biological and biomedical applications. Copyright © 2016 John Wiley & Sons, Ltd.
Purification and Characterization of Glucose 6‐Phosphate Dehydrogenase, 6‐Phosphogluconate Dehydrogenase, and Glutathione Reductase from Rat Heart and Inhibition Effects of Furosemide, Digoxin, and Dopamine on the Enzymes Activities
Authors: Adem Sevki , Ciftci Mehmet 
Published: January 2016

ABSTRACTThe present study was aimed to investigate characterization and purification of glucose–6‐phosphate dehydrogenase, 6‐phosphogluconate dehydrogenase, and glutathione reductase from rat heart and the inhibitory effect of three drugs. The purification of the enzymes was performed using 2',5'‐ADP sepharose 4B affinity material. The subunit and the natural molecular weights were analyzed by SDS‐PAGE and gel filtration. Biochemical characteristics such as the optimum temperature, pH, stable pH, and salt concentration were examined for each enzyme. Types of product inhibition and Ki values with Km and Vmax values of the substrates and coenzymes were determined. According to the obtained Ki and IC50 values, furosemide, digoxin, and dopamine showed inhibitory effect on the enzyme activities at low millimolar concentrations in vitro conditions. Dopamine inhibited the activity of these enzymes as competitive, whereas furosemide and digoxin inhibited the activity of the enzyme as noncompetitive.
Computational Results for Regular Difference Systems of Sets Attaining or Being Close to the Levenshtein Bound
Authors: Chisaki Shoko , Miyamoto Nobuko 
Published: January 2016

AbstractDifference systems of sets (DSSs) are combinatorial structures arising in connection with code synchronization that were introduced by Levenshtein in 1971, and are a generalization of cyclic difference sets. In this paper, we consider a collection of m‐subsets in a finite field of prime order to be a regular DSS for an integer m, and give a lower bound on the parameter ρ of the DSS using cyclotomic numbers. We show that when we choose ‐subsets from the multiplicative group of order e, the lower bound on ρ is independent of the choice of subsets. In addition, we present some computational results for DSSs with block sizes and , whose parameter ρ attains or comes close to the Levenshtein bound for .
Subcellular localization of calcium deposits in the noble crayfish Astacus astacus spermatophore: Implications for post‐mating spermatophore hardening and spermatozoon maturation
Authors: Niksirat Hamid , Kouba Antonín 
Published: January 2016

ABSTRACTThe freshly ejaculated spermatophore of crayfish undergoes a hardening process during post‐mating storage on the body surface of female. The ultrastructural distribution of calcium deposits were studied and compared in freshly ejaculated and post‐mating noble crayfish spermatophores, using the oxalate‐pyroantimonate technique, to determine possible roles of calcium in post‐mating spermatophore hardening and spermatozoon maturation. Small particles of sparsely distributed calcium deposits were visible in the wall of freshly ejaculated spermatophore. Also, large amount of calcium deposits were visible in the membranes of the freshly ejaculated spermatozoon. Five minutes post‐ejaculation, granules in the spermatophore wall appeared as porous formations with numerous electron lucent spaces. Calcium deposits were visible within the spaces and scattered in the spermatophore wall matrix, where smaller calcium deposits combined to form globular calcium deposits. Large numbers of the globular calcium deposits were visible in the wall of the post‐mating spermatophore. Smaller calcium deposits were detected in the central area of post‐mating spermatophore, which contains the sperm mass, and in the extracellular matrix and capsule. While the density of calcium deposits decreased in the post‐mating spermatozoon membranes, numerous small calcium deposits appeared in the subacrosomal zone and nucleus. Substantial changes in calcium deposit distribution in the crayfish spermatophore during post‐mating storage on the body of female may be involved in the processes of the spermatophore hardening and spermatozoon maturation. J. Morphol. 277:445–452, 2016. © 2016 Wiley Periodicals, Inc.