bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profile of Reproductive Issues Associated with Different Sickle Cell Disease Genotypes
Authors: Carvalho Flávia Anchielle , Souza Ariani Impieri , Ferreira Ana Laura Carneiro Gomes , Neto Simone da Silva , Oliveira Ana Carolina Pessoa de Lima , Gomes Maria Luiza Rodrigues Pinheiro , Costa Manuela Freire Hazin 
Published: July 2017

Purpose To describe the reproductive variables associated with different sickle cell disease (SCD) genotypes and the influence of contraceptive methods on acute painful episodes among the women with the homozygous hemoglobin S (HbSS) genotype.
Methods A cross-sectional study was conducted between September of 2015 and April of 2016 on 158 women afflicted with SCD admitted to a hematology center in the Northeast of Brazil. The reproduction-associated variables of different SCD genotypes were assessed using the analysis of variance (ANOVA) test to compare means, and the Kruskal-Wallis test to compare medians. The association between the contraceptive method and the acute painful episodes was evaluated by the Chi-square test.
Results The mean age of women with SCD was 28.3 years and 86.6% were mixed or of African-American ethnicity. With respect to the genotypes, 134 women (84.8%) had HbSS genotype, 12 women (7.6%) had hemoglobin SC (HbSC) disease genotype, and 12 (7.6%) were identified with hemoglobinopathy S-beta (S-β) thalassemia. The mean age of HbSS diagnosis was lower than that of HbSC disease, the less severe form of SCD (p < 0.001). The mean age of menarche was 14.8 ± 1.8 years for HbSS and 12.7 ± 1.5 years for HbSC (p < 0.001). Among women with HbSS who used progestin-only contraception, 16.6% had more than 4 acute painful episodes per year. There was no statistically significant difference when compared with other contraceptive methods.
Conclusion With respect to reproduction-associated variables, only the age of the menarche showed delay in HbSS when compared with HbSC. The contraceptive method used was not associated with the frequency of acute painful episodes among the HbSS women.
How Low Is Too Low? Postpartum Hemorrhage Risk among Women with Thrombocytopenia
Authors: Dotters-Katz Sarah , Smid Marcela , Manuck Tracy , Carlson Laura 
Published: July 2017

Objective To estimate the association between severity of thrombocytopenia and postpartum hemorrhage.
Study Design We performed a secondary analysis of a prospective cohort of women delivering by cesarean or vaginal birth after cesarean conducted by the National Institute of Child Health and Human Development (NICHD) Maternal–Fetal Medicine Unit. Women delivering ≥ 20 weeks with platelets < 400,000/mL were included. Thrombocytopenia was defined as predelivery platelets of < 150,000/mL. Primary outcomes were (1) laboratory evidence of hemorrhage, defined as a decrease in hemoglobin ≥ 4 mg/dL and (2) clinical evidence of hemorrhage, a composite of atony, transfusion, coagulopathy, hysterectomy, laparotomy, or intensive care unit admission. Odds ratios were calculated for primary outcomes using thrombocytopenia as a dichotomous and ordinal variable.
Results A total of 54,597 women were included; 5,611 (10.3%) had antepartum thrombocytopenia, 1,976 (3.6%) women had laboratory evidence of hemorrhage, and 3,862 (7.1%) had clinical evidence of hemorrhage. Thrombocytopenia was associated with both laboratory evidence of hemorrhage (adjusted odds ratio [aOR]: 1.60, 95% CI: 1.38–1.86) and clinical evidence of hemorrhage (aOR: 1.68, 95% CI: 1.52–1.83). The odds of laboratory and clinical evidence of hemorrhage increased incrementally with severity of thrombocytopenia.
Conclusion Thrombocytopenia is associated with both laboratory and clinical evidence of hemorrhage; risk increases dramatically as platelet count decreases.
Die infizierte Hüfttotalendoprothese: Eine Onlineumfrage zum aktuellen diagnostischen und therapeutischen Vorgehen in 107 deutschen Krankenhäusern
Authors: Langenmair Elia , Konstantinidis Lukas , Südkamp Norbert , Helwig Peter 
Published: July 2017

Zusammenfassung
Hintergrund Der Hüftprotheseninfekt ist eine häufige und schwerwiegende Komplikation mit weitreichenden sozioökonomischen Konsequenzen. Die inkonsistente Datenlage sowie das Fehlen verbindlicher Leitlinien führen zu unterschiedlichen diagnostischen und therapeutischen Behandlungsstrategien. Ziel dieser Arbeit ist es, das aktuelle diagnostische und therapeutische Vorgehen bei Hüft-TEP-Infekt in deutschen Krankenhäusern darzustellen, dieses mit der aktuellen Studienlage zu verknüpfen und die Bereitschaft deutscher Kliniken zur Beteiligung an prospektiven multizentrischen Studien zu evaluieren.
Material und Methoden Ein Onlinefragebogen wurde zur digitalen Bearbeitung via E-Mail an endoprothetisch tätige Krankenhäuser und Kliniken versandt. Das Spektrum umfasste Kreiskrankenhäuser, Kliniken mit privaten Trägern, nicht universitäre Maximalversorger, berufsgenossenschaftliche Kliniken (BG-Kliniken) und Universitätskliniken.
Ergebnisse Es beteiligten sich 107 Kliniken an der Umfrage, das entspricht einer Beteiligungsrate von 27,6%. Diese Kliniken führen jährlich ca. 2951 septische Hüft-TEP-Wechsel durch. Der zweizeitige septische Hüft-TEP-Wechsel ist das bevorzugte Vorgehen beim septischen Hüft-TEP-Wechsel. Der Algorithmus nach Zimmerli und Kollegen findet weitläufig Anerkennung. Bezüglich zentraler Inhalte der septischen Revisionsendoprothetik – langes vs. kurzes Intervall im Rahmen des zweizeitigen Hüft-TEP-Wechsels, Dauer der intravenösen und oralen Antibiose, zementierte vs. nicht zementierte Implantationsverfahren, Nachuntersuchungsintervalle – besteht ein inhomogenes intra- und interklinisches Vorgehen. Es konnte eine hohe Bereitschaft zur Teilnahme an klinischen Studien festgestellt werden.
Schlussfolgerung Die kontroverse Studienlage resultiert in multiplen therapeutischen Ansätzen. Die hohe Bereitschaft zur Studienteilnahme bei entsprechender Finanzierung bietet das Potenzial zur Realisierung multizentrischer Forschungsvorhaben. Eine entsprechende finanzielle Forschungsförderung ist in diesem Zusammenhang zwingend erforderlich.
SAPV: Spezialisierte Ambulante Palliativversorgung
Authors: Hartwig Maite 
Published: July 2017

SummarySeit 2007 ist im Sozialgesetzbuch festgeschrieben, dass Versicherte mit einer nicht heilbaren, fortschreitenden und weit fortgeschrittenen Erkrankung, begrenzter Lebenserwartung, die besonders aufwändige Versorgung benötigen, Anspruch auf spezialisierte ambulante Palliativversorgung (SAPV) haben.Der Beitrag gibt einen kompakten Überblick zur SAPV und geht auf die Besonderheiten der Versorgung von Kindern ein.
Wir müssen in allem, was wir mit dem Patienten besprechen, wahrhaftig sein
Authors: Niklas Anke 
Published: July 2017

SummaryProf. Dr. med. Claudia Bausewein ist Direktorin der Klinik und Poliklinik für Palliativmedizin am Klinikum der Universität München. Sie leitet die Christophorus Akademie für Palliativmedizin, Palliativpflege und Hospizarbeit in München und ist Visiting Professor am Cicely Saunders Institute, King’s College London.Im Interview erzählt sie u. a., warum sich die Patientenverfügung überholt hat, wo Advance Care Planning ansetzt und wie eine gute palliativmedizinische Betreuung Sterbewünsche Schwerstkranker in den allermeisten Fällen zum Verschwinden bringt.
Fallbericht: Neuraltherapie und Lavendelöl bei persistierendem Ulcus cruris
Authors: Lachmann Michael 
Published: July 2017

SummaryEin seit Monaten persistierendes Ulcus cruris venosum wurde über einen Zeitraum von 2 Monaten alle 3 Tage mit Procain und einem Lavendelölverband behandelt. Bereits einige Tage nach Therapiebeginn zeigten sich erste Granulationen des bisher avitalen Ulkus. Nach wenigen Wochen präsentierte sich ein epithealisiertes Ulcus cruris.
Kleine Patienten ganz groß
Authors: Bittner Sebastian 
Published: July 2017

Zusammenfassung
Pflegerische Besonderheiten in der Kinderanästhesie Fast jede Fortbildung und auch fast jeder Artikel zum Thema Anästhesie bei
Kindern/Säuglingen startet mit den Worten „Kinder sind keine kleinen Erwachsenen!“. Doch was verbirgt sich dahinter? Was sind anatomische
Besonderheiten und was sind die Anforderungen an die psychische Betreuung von Kindern? In welche Altersgruppen lassen sich „Kinder“ klinisch
relevant einteilen? Dieser CNE-Schwerpunkt soll eine Zusammenfassung der wichtigsten und pflegerischen Besonderheiten der kleinen Patienten
aufzeigen und geht besonders auf die vorbereitenden Maßnahmen ein.
Extrakranielle Schwannome im Kopf-Hals-Bereich: Klinische Implikationen anhand von 20 Fällen
Authors: Höing Benedikt , Stuck Boris A. , Kansy Benjamin , Hussain Timon , Schlüter Anke , Holtmann Laura , Ringelstein Adrian , Mattheis Stefan , Lang Stephan , Dominas Nina 
Published: July 2017

Zusammenfassung
Hintergrund Schwannome sind seltene, benigne Tumore, die von den Gliazellen des peripheren Nervensystems (Schwannsche Zellen)
ausgehen. Während es für intrakranielle Schwannome, insbesondere die des N. vestibularis, etablierte Therapiestrategien gibt, liegen zu den
extrakraniellen Schwannomen weniger belastbare Daten vor.
Methode Es wurde eine retrospektive Analyse von 20 Patienten mit histopathologisch gesicherten extrakraniellen Schwannomen im
Kopf-Hals-Bereich mit einem Schwerpunkt auf Tumorlokalisation und postoperativem funktionellem Therapieergebnis durchgeführt.
Ergebnisse Die Schwannome im vorliegenden Kollektiv entstammten vornehmlich dem N. facialis (n = 4), dem N. vagus (n = 4) oder dem
sympathischen Grenzstrang (n = 3). Die häufigsten Primärsymptome waren Schwellungen (n = 12) und Schmerzen (n = 3). MRT (n = 13),
Ultraschall (n = 12) und CT (n = 3) wurden zur Diagnostik eingesetzt. In 18 Fällen wurde ein chirurgischer Behandlungsansatz gewählt (n = 14
komplette Exstirpation, n = 3 Teilexstirpation, n = 1 unbekannter Resektionsumfang). Komplette Schwannomexstirpationen von
motorisch-efferentenNerven (n = 10) machten ein Absetzen des Nervens häufiger erforderlich, wenn bereits präoperativ ein motorisches Defizit
bestand (3 von 4 = 75 % bei präoperativem Defizit vs. 2 von 6 = 33 % ohne präoperatives Defizit).
Schlussfolgerung Extrakranielle Schwannome stellen seltene, meist vom N. facialis, dem sympathischen Grenzstrang oder den kaudalen
Hirnnerven ausgehende, benigne Tumore des HNO-Trakts dar, die in der Regel chirurgisch therapiert werden. Mit postoperativen motorischen
Defiziten bei kompletten Exstirpationen muss insbesondere bei bereits präoperativ bestehenden Funktionsstörungen gerechnet werden.
Keratokonus: heutige Therapieoptionen
Authors: Raiskup Frederik , Lenk Janine , Herber Robert , Gatzioufas Zisis , Spörl Eberhard 
Published: July 2017

ZusammenfassungDie Inzidenz des Keratokonus wird in aktuellen Studien deutlich höher geschätzt als bisher angenommen, somit wird diese Hornhautektasie nicht mehr zu den seltenen Erkrankungen gezählt. Als einer der bedeutendsten Risikofaktoren für die Entstehung des Keratokonus wurde neben Atopie und Down-Syndrom das chronische Augenreiben definiert. Dies ist der durch Verhaltenstraining und konsequente Aufklärung einzig wirklich modifizierbare Risikofaktor. Weitere Therapieoptionen beinhalten Maßnahmen zur Visusverbesserung wie die Anpassung von Brillen und formstabilen Kontaktlinsen, die Implantation kornealer Ringsegmente oder phaker intraokularer Linsen. Im Falle einer Krankheitsprogression bietet das korneale Crosslinking ein effektives und sicheres Verfahren, sodass das Erfordernis einer Hornhauttransplantation in dieser Indikation immer seltener wird. Neuere Studien sehen auch einen positiven Effekt einer Kombination der bisher bei Keratokonus kontraindizierten photorefraktiven Keratektomie und dem Crosslinking. Die perforierende Keratoplastik stellt allerdings weiterhin den Goldstandard der chirurgischen Behandlung des Keratokonus im fortgeschrittenen Stadium dar, wobei in den letzten Jahren auch die lamellären Verfahren immer mehr in den Vordergrund rücken.
Comparison of B-Mode with B-Flow Sonography for the Evaluation of Femoral Arteries in Infants
Authors: Groth Michael , Dammann Elena , Arndt Florian , Ernst Marielle , Herrmann Jochen 
Published: July 2017

Purpose To evaluate femoral arteries (FAs) in infants in the context of catheter angiography with B-mode (BMS) and B-flow sonography (BFS) and to compare both methods for vessel delineation and reliability of vessel diameter measurements.
Methods 21 consecutive infants who underwent ultrasound for the evaluation of FAs before or after cardiac catheterization were retrospectively included in this study. The diameter of the FAs and the maximum length of the vessel section displayed on a single ultrasound plane were recorded by two radiologists for BMS and BFS. The visual image quality was rated by one observer. Statistics included intraclass correlation coefficient, Bland-Altman analysis, Fisher´s exact test and t-test.
Results The intraobserver as well as interobserver correlation was excellent for BMS (0.7922 and 0.6521) and BFS (0.8094 and 0.7637). The Bland-Altman analysis revealed limits of agreement for BMS between +/–0.73 mm (intraobserver) and +/–0.55 mm (interobserver) and for BFS between +/–0.83 mm (intraobserver) and +/–0.7 mm (interobserver). BFS allowed visualization of a longer stretch of the FA than BMS (length 3.54 +/–0.85 vs. 2.21 +/–0.9 cm, p < 0.0001). The image quality was significantly higher for BFS (p = 0.0043).
Conclusion Since BFS shows excellent reproducibility for vessel measurements and superior image quality in infants, if available, BFS should be included in standard protocols when realistic measurements are required.
Key Points
Citation Format
Trends in mica–mica adhesion reflect the influence of molecular details on long-range dispersion forces underlying aggregation and coalignment
Authors: Li Dongsheng , Chun Jaehun , Xiao Dongdong , Zhou Weijiang , Cai Huacheng , Zhang Lei , Rosso Kevin M. , Mundy Christopher J. , Schenter Gregory K. , De Yoreo James J. 
Published: July 2017

Significance
Crystal growth through nanoparticle assembly appears to be a common phenomenon in synthetic, biological, and geochemical settings, and can lead to formation of hierarchical structures with unique properties tied to this nonclassical assembly process, but the underlying forces driving assembly are poorly understood. Using custom-made single-crystal atomic force microscopy tips, we measured the adhesion force between mica surfaces in the solvent-separate regime as a function of crystallographic orientation and solution parameters. The observed dependencies are predicted using a continuum description of the electrostatic and electrodynamic interactions that includes short-range molecular details. The findings show that the attractive interaction driving nanoparticle assembly and orientation results from the coupling between the short-range solution response and the long-range van der Waals interactions.
Mechanism-based inactivator of isocitrate lyases 1 and 2 fromMycobacterium tuberculosis
Authors: Pham Truc V. , Murkin Andrew S. , Moynihan Margaret M. , Harris Lawrence , Tyler Peter C. , Shetty Nishant , Sacchettini James C. , Huang Hsiao-ling , Meek Thomas D. 
Published: July 2017

SignificanceTuberculosis, caused byMycobacterium tuberculosis(Mtb) bacteria, is the most prevalent infectious disease, affecting one-third of the global population, especially in developing countries. First-line therapies to treat this disease are losing efficacy due to the emergence of drug resistance. Accordingly, new therapeutic agents of novel mechanisms of action remain an urgent medical need. The isocitrate lyases (ICL1 and ICL2) comprise metabolically essential enzymes ofMtb, are absent in mammals, and thereby provide therapeutically important drug targets for tuberculosis. Here, we describe the first example of a mechanism-based inactivator of ICL1 and ICL2 that could provide a starting point for the development of new drugs to treat tuberculosis.
The domestications and the domesticators of Asian rice
Authors: van Driem George 
Published: July 2017

Abstract
Rice genetics has now provided molecular evidence for three distinct domestications of Asian rice, giving rise to ahu, indica and japonica rice and subsequently involving the multidirectional introgression of favoured alleles between these three families of Oryza sativa cultivars. The phylogeography of Asian wild and cultivated rice species also permits inferences with regard to the likely geographical range within which these three domestication processes involving Asian cultivated rice unfolded. Evidence from linguistic palaeontology permits the identification of two language families whose linguistic ancestors pose the likeliest candidates for the earliest rice domesticators, Austroasiatic and Hmong-Mien. The linguistic arguments and population genetic evidence on Asian rice are assessed. Recent advances in palaeobotany as well as a number of currently prevalent misunderstandings in rice archaeology are discussed. Another set of evidence from linguistic palaeontology involving reconstructible etyma denoting megafauna in light of the early Holocene distribution of these megafaunal species provides a geographical indication for the location of the early Austroasiatic homeland. Furthermore, the molecular genetics of human populations are discussed in order to shed light on the prehistory and geography of the Austroasiatic, Hmong-Mien and other language families. Finally, a synthesis of the disparate sets of evidence is presented.
DOOR: a prokaryotic operon database for genome analyses and functional inference
Authors: Cao Huansheng , Ma Qin , Chen Xin , Xu Ying 
Published: July 2017

AbstractThe rapid accumulation of fully sequenced prokaryotic genomes provides unprecedented information for biological studies of bacterial and archaeal organisms in a systematic manner. Operons are the basic functional units for conducting such studies. Here, we review an operon database DOOR (the Database of prOkaryotic OpeRons) that we have previously developed and continue to update. Currently, the database contains 6 975 454 computationally predicted operons in 2072 complete genomes. In addition, the database also contains the following information: (i) transcriptional units for 24 genomes derived using publicly available transcriptomic data; (ii) orthologous gene mapping across genomes; (iii) 6408 cis-regulatory motifs for transcriptional factors of some operons for 203 genomes; (iv) 3 456 718 Rho-independent terminators for 2072 genomes; as well as (v) a suite of tools in support of applications of the predicted operons. In this review, we will explain how such data are computationally derived and demonstrate how they can be used to derive a wide range of higher-level information needed for systems biology studies to tackle complex and fundamental biology questions.
VCF-Explorer: filtering and analysing whole genome VCF files
Authors: Akgün Mete , Demirci Hüseyin 
Published: July 2017

Abstract

Summary
The decreasing cost in high-throughput technologies led to a number of sequencing projects consisting of thousands of whole genomes. The paradigm shift from exome to whole genome brings a significant increase in the size of output files. Most of the existing tools which are developed to analyse exome files are not adequate for larger VCF files produced by whole genome studies. In this work we present VCF-Explorer, a variant analysis software capable of handling large files. Memory efficiency and avoiding computationally costly pre-processing step enable to carry out the analysis to be performed with ordinary computers. VCF-Explorer provides an easy to use environment where users can define various types of queries based on variant and sample genotype level annotations. VCF-Explorer can be run in different environments and computational platforms ranging from a standard laptop to a high performance server.

Availability and implementation
VCF-Explorer is freely available at: http://vcfexplorer.sourceforge.net/.

Supplementary information
Supplementary data are available at Bioinformatics online.
DynaPho: a web platform for inferring the dynamics of time-series phosphoproteomics
Authors: Hsu Chia-Lang , Wang Jian-Kai , Lu Pei-Chun , Huang Hsuan-Cheng , Juan Hsueh-Fen 
Published: July 2017

Abstract

Summary
Large-scale phosphoproteomics studies have improved our understanding of dynamic cellular signaling, but the downstream analysis of phosphoproteomics data is still a bottleneck. We develop DynaPho, a useful web-based tool providing comprehensive and in-depth analyses of time-course phosphoproteomics data, making analysis intuitive and accessible to non-bioinformatics experts. The tool currently implements five analytic modules, which reveal the transition of biological pathways, kinase activity, dynamics of interaction networks and the predicted kinase-substrate associations. These features can assist users in translating their larger-scale time-course phosphoproteomics data into valuable biological discoveries.

Availability and implementation
DynaPho is freely available at http://dynapho.jhlab.tw/.

Supplementary information
Supplementary data are available at Bioinformatics online.
A Technique of Awake Bronchoscopic Endotracheal Intubation for Respiratory Failure in Patients With Right Heart Failure and Pulmonary Hypertension
Authors: Johannes Jimmy , Berlin David A. , Patel Parimal , Schenck Edward J. , West Frances Mae , Saggar Rajan , Barjaktarevic Igor Z. 
Published: July 2017

Objective:
Patients with pulmonary hypertension and right heart failure have a high risk of clinical deterioration and death during or soon after endotracheal intubation. The effects of sedation, hypoxia, hypoventilation, and changes in intrathoracic pressure can lead to severe hemodynamic instability. In search for safer approach to endotracheal intubation in this cohort of patients, we evaluate the safety and feasibility of an alternative intubation technique.

Data Sources:
Retrospective data analysis.

Study Selection:
Two medical ICUs in large university hospitals in the United States.

Data Extraction:
We report a case series of nine nonconsecutive patients with compromised right heart function, pulmonary hypertension, and severe acute hypoxemic respiratory failure who underwent endotracheal intubation with a novel technique combining awake bronchoscopic intubation supported with nasally delivered noninvasive positive pressure ventilation or high-flow nasal cannula.

Data Synthesis:
All patients were intubated in the first attempt without major complications and eight patients (88%) were alive 24 hours after intubation. Systemic hypotension was the most frequent complication following the procedure.

Conclusions:
Awake bronchoscopic intubation supported with a noninvasive positive pressure delivery systems may be feasible alternative to standard direct laryngoscopy approach. Further studies are needed to better assess its safety and applicability.