bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Erythrocyte Binding Protein of Plasmodium vivax Suggests an Alternate Invasion Pathway into Duffy-Positive Reticulocytes
Authors: Ntumngia Francis B. , Thomson-Luque Richard , Torres Letícia de Menezes , Gunalan Karthigayan , Carvalho Luzia H. , Adams John H. 
Published: August 2016

ABSTRACT

Erythrocyte invasion by malaria parasites is essential for blood-stage development and an important determinant of host range. In
Plasmodium vivax
, the interaction between the Duffy binding protein (DBP) and its cognate receptor, the Duffy antigen receptor for chemokines (DARC), on human erythrocytes is central to blood-stage infection. Contrary to this established pathway of invasion, there is growing evidence of
P. vivax
infections occurring in Duffy blood group-negative individuals, suggesting that the parasite might have gained an alternative pathway to infect this group of individuals. Supporting this concept, a second distinct erythrocyte binding protein (EBP2), representing a new member of the DBP family, was discovered in
P. vivax
and may be the ligand in an alternate invasion pathway. Our study characterizes this novel ligand and determines its potential role in reticulocyte invasion by
P. vivax
merozoites
.
EBP2 binds preferentially to young (CD71
high
) Duffy-positive (Fy
+
) reticulocytes and has minimal binding capacity for Duffy-negative reticulocytes. Importantly, EBP2 is antigenically distinct from DBP and cannot be functionally inhibited by anti-DBP antibodies. Consequently, our results do not support EBP2 as a ligand for invasion of Duffy-negative blood cells, but instead, EBP2 may represent a novel ligand for an alternate invasion pathway of Duffy-positive reticulocytes.

IMPORTANCE
For decades,
P. vivax
infections in humans have been defined by a unique requirement for the interaction between the Duffy binding protein ligand of the parasite and the Duffy blood group antigen receptor (DARC). Recent reports of
P. vivax
infections in Duffy-negative individuals challenge this paradigm and suggest an alternate pathway of infection, potentially using the recently discovered EBP2. However, we demonstrate that EBP2 host cell specificity is more restricted than DBP binding and that EBP2 binds preferentially to Duffy-positive, young reticulocytes. This finding indicates that this DBP paralog does mediate a Duffy-independent pathway of infection.
Abyssal origin for the early Holocene pulse of unradiogenic neodymium isotopes in Atlantic seawater
Authors: Howe Jacob N.W. , Piotrowski Alexander M. , Rennie Victoria C.F. 
Published: August 2016

Abstract
The neodymium isotopic composition of authigenic phases of deep-sea sediment cores can be interpreted as reflecting past changes in water-mass mixing proportions if end-member water-mass compositions are constrained through time. Here we present three new records spanning 2480 to 4360 m depth in the North Atlantic Ocean that show seawater Nd isotope values in the early to mid-Holocene that are more radiogenic than values from the abyssal northwest Atlantic. This finding indicates that that the end-member composition of North Atlantic Deep Water was more stable within its core than it was at abyssal depths. The spatial distribution of the unradiogenic neodymium isotope values observed in the North Atlantic suggests a bottom source, and therefore that they were unlikely to have been due to the production of intermediate-depth Labrador Sea Water. We infer that the unradiogenic authigenic Nd isotope values were most likely derived from a pulse of poorly chemically weathered detrital material that was deposited into the Labrador Sea following Laurentide ice sheet retreat in the early Holocene. This unradiogenic sediment released neodymium into the bottom waters, yielding an unradiogenic seawater signal that was advected southward at abyssal depths and attenuated as it vertically mixed upward in the water column to shallower depths. The southward dispersion of these unradiogenic seawater values traces deep-water advection. However, the exact values observed at the most abyssal sites cannot be interpreted as proportionate to the strength of deep-water production without improved constraints on end-member changes.
Extensive Noachian fluvial systems in Arabia Terra: Implications for early Martian climate
Authors: Davis J.M. , Balme M. , Grindrod P.M. , Williams R.M.E. , Gupta S. 
Published: August 2016

Abstract
Valley networks are some of the strongest lines of evidence for extensive fluvial activity on early (Noachian; &gt;3.7 Ga) Mars. However, their purported absence on certain ancient terrains, such as Arabia Terra, is at variance with patterns of precipitation as predicted by “warm and wet” climate models. This disagreement has contributed to the development of an alternative “icy highlands” scenario, whereby valley networks were formed by the melting of highland ice sheets. Here, we show through regional mapping that Arabia Terra shows evidence for extensive networks of sinuous ridges. We interpret these ridge features as inverted fluvial channels that formed in the Noachian, before being subject to burial and exhumation. The inverted channels developed on extensive aggrading flood plains. As the inverted channels are both sourced in, and traverse across, Arabia Terra, their formation is inconsistent with discrete, localized sources of water, such as meltwater from highland ice sheets. Our results are instead more consistent with an early Mars that supported widespread precipitation and runoff.
Social Autopsy of maternal, neonatal deaths and stillbirths in rural Bangladesh: qualitative exploration of its effect and community acceptance
Authors: Biswas Animesh , Rahman Fazlur , Eriksson Charli , Halim Abdul , Dalal Koustuv 
Published: August 2016

ObjectivesSocial Autopsy (SA) is an innovative strategy where a trained facilitator leads community groups through a structured, standardised analysis of the physical, environmental, cultural and social factors contributing to a serious, non-fatal health event or death. The discussion stimulated by the formal process of SA determines the causes and suggests preventative measures that are appropriate and achievable in the community. Here we explored individual experiences of SA, including acceptance and participant learning, and its effect on rural communities in Bangladesh. The present study had explored the experiences gained while undertaking SA of maternal and neonatal deaths and stillbirths in rural Bangladesh.DesignQualitative assessment of documents, observations, focus group discussions, group discussions and in-depth interviews by content and thematic analyses.ResultsEach community's maternal and neonatal death was a unique, sad story. SA undertaken by government field-level health workers were well accepted by rural communities. SA had the capability to explore the social reasons behind the medical cause of the death without apportioning blame to any individual or group. SA was a useful instrument to raise awareness and encourage community responses to errors within the society that contributed to the death. People participating in SA showed commitment to future preventative measures and devised their own solutions for the future prevention of maternal and neonatal deaths.ConclusionsSA highlights societal errors and promotes discussion around maternal or newborn death. SA is an effective means to deliver important preventative messages and to sensitise the community to death issues. Importantly, the community itself is enabled to devise future strategies to avert future maternal and neonatal deaths in Bangladesh.
Study to Improve Cardiovascular Outcomes in high-risk older patieNts (ICON1) with acute coronary syndrome: study design and protocol of a prospective observational study
Authors: Kunadian Vijay , Neely R Dermot G , Sinclair Hannah , Batty Jonathan A , Veerasamy Murugapathy , Ford Gary A , Qiu Weiliang 
Published: August 2016

IntroductionThe ICON1 study (a study to Improve Cardiovascular Outcomes in high-risk older patieNts with acute coronary syndrome) is a prospective observational study of older patients (≥75 years old) with non-ST-elevation acute coronary syndrome managed by contemporary treatment (pharmacological and invasive). The aim of the study was to determine the predictors of poor cardiovascular outcomes in this age group and to generate a risk prediction tool.Methods and analysisParticipants are recruited from 2 tertiary hospitals in the UK. Baseline evaluation includes frailty, comorbidity, cognition and quality-of-life measures, inflammatory status assessed by a biomarker panel, including microRNAs, senescence assessed by telomere length and telomerase activity, cardiovascular status assessed by arterial stiffness, endothelial function, carotid intima media thickness and left ventricular systolic and diastolic function, and coronary plaque assessed by virtual histology intravascular ultrasound and optical coherence tomography. The patients are followed-up at 30 days and at 1 year for primary outcome measures of death, myocardial infarction, stroke, unplanned revascularisation, bleeding and rehospitalisation.Ethics and disseminationThe study has been approved by the regional ethics committee (REC 12/NE/016). Findings of the study will be presented in scientific sessions and will be published in peer-reviewed journals.Trial registration numberNCT01933581: Pre-results.
Association between hyperglycaemic crisis and long-term major adverse cardiovascular events: a nationwide population-based, propensity score-matched, cohort study
Authors: Chang Li-Hsin , Lin Liang-Yu , Tsai Ming-Tsun , How Chorng-Kuang , Chiang Jen-Huai , Hsieh Vivian Chia-Rong , Hu Sung-Yuan , Hsieh Ming-Shun 
Published: August 2016

ObjectiveHyperglycaemic crisis was associated with significant intrahospital morbidity and mortality. However, the association between hyperglycaemic crisis and long-term cardiovascular outcomes remained unknown. This study aimed to investigate the association between hyperglycaemic crisis and subsequent long-term major adverse cardiovascular events (MACEs).Participants and methodsThis population-based cohort study was conducted using data from Taiwan's National Health Insurance Research Database for the period of 1996–2012. A total of 2171 diabetic patients with hyperglycaemic crisis fit the inclusion criteria. Propensity score matching was used to match the baseline characteristics of the study cohort to construct a comparison cohort which comprised 8684 diabetic patients without hyperglycaemic crisis. The risk of long-term MACEs was compared between the two cohorts.ResultsSix hundred and seventy-six MACEs occurred in the study cohort and the event rate was higher than that in the comparison cohort (31.1% vs 24.1%, p<0.001). Patients with hyperglycaemic crisis were associated with a higher risk of long-term MACEs even after adjusting for all baseline characteristics and medications (adjusted HR=1.76, 95% CI 1.62 to 1.92, p<0.001). Acute myocardial infarction had the highest adjusted HR (adjusted HR=2.19, 95% CI 1.75 to 2.75, p<0.001) in the four types of MACEs, followed by congestive heart failure (adjusted HR=1.97, 95% CI 1.70 to 2.28, p<0.001). Younger patients with hyperglycaemic crisis had a higher risk of MACEs than older patients (adjusted HR=2.69 for patients aged 20–39 years vs adjusted HR=1.58 for patients aged >65 years).ConclusionsHyperglycaemic crisis was significantly associated with long-term MACEs, especially in the young population. Further prospective longitudinal study should be conducted for validation.
Association between adiposity and systemic atherosclerosis: a protocol of a cross-sectional autopsy study
Authors: Nishizawa Aline , Suemoto Claudia Kimie , Farias Daniela Souza , Campos Fernanda Marinho , Silva Karen Cristina Souza da , Cuelho Anderson , Leite Renata Elaine Paraízo , Ferretti-Rebustini Renata Eloah de Lucena , Grinberg Lea Tenenholz , Farfel José Marcelo , Jacob-Filho Wilson , Pasqualucci Carlos Augusto 
Published: August 2016

IntroductionAdiposity has been associated with atherosclerosis in clinical studies. However, few autopsy studies have investigated this association, and they had only examined the coronary artery disease. Moreover, most studies had small sample sizes and were limited to middle-aged or young adults. Our aim is to investigate the association between adiposity and systemic atherosclerosis in an autopsy study.Methods and analysisA sample of 240 deceased with 30 years or more will be evaluated. The sample size was calculated using the lowest correlation coefficient found in previous studies (r=0.109), assuming a power of 90% and α=0.05. We will collect information about sociodemographics, frequency of previous contact of the deceased's next of kin and cardiovascular risk factors. We will measure neck, waist and hip circumferences, weight, height and abdominal subcutaneous tissue thickness, and then we will calculate the body mass index, waist-to-hip ratio, waist-to-height ratio and body shape index. We will also weigh the pericardial and abdominal visceral fat, the heart, and we will measure the left ventricular wall thickness. We will evaluate the presence of myocardial infarction, the degree of atherosclerosis in the aorta, carotid, coronary and cerebral arteries and plaque composition in carotid, coronary and cerebral arteries. For each individual, we will fix arterial and adipose tissue samples in 10% formalin and freeze another adipose tissue sample at −80°C for future studies.Ethics and disseminationEthical approval was granted by the Ethics Committee of University of Sao Paulo Medical School, Brazil. Results will be submitted for publication in a peer-reviewed journal.
Absence of significant adverse events following thalidomide administration in bitches diagnosed with mammary gland carcinomas
Authors: de Campos C. B. , Lavalle G. E. , Fialho Ligório S , Camargo Nunes F. , Carneiro R. A. , Amorim R. L. , Cassali G. D. 
Published: August 2016

The aim of the study was to evaluate the incidence of adverse events (AEs) in female dogs diagnosed with advanced clinical stage mammary gland neoplasms following treatment with thalidomide. A prospective analysis of 29 female dogs treated with a high dose (HD) of 20 mg/kg/day of thalidomide for three months followed by a low dose (LD) of 10 mg/kg/day of thalidomide for three months was performed. All patients underwent physical examination, complete blood count, serum biochemistry profile, thoracic radiographs, and abdominal ultrasound analysis before the treatment and after the HD and LD. Clinical AEs were absent in 16/29 (55.17 per cent) patients following HD. An initial 3–5 day period of somnolence was described in 4/29 (13.79 per cent), prolonged somnolence in 5/29 (17.24 per cent), a short period of somnolence lasting only a few hours in 3/29 (10.34 per cent), and difficulty to rouse was described in 5/29 (17.24 per cent) cases. Two patients (6.89 per cent) presented with prolonged somnolence that interfered with activities of daily living, resulting in anticipation of the dose reduction to the proposed LD after 15 days of the HD treatment. Following dose reduction, AE improvement was observed in all patients. Albeit remaining within the reference ranges, erythrocytes, haematocrit, total leucocyte count, neutrophils, lymphocytes, monocytes and γ‐glutamyltranspeptidase showed significant alteration associated to thalidomide treatment.
Effects of oil field brine wastewater on saturated hydraulic conductivity of smectitic loam soils
Authors: Derby Nathan E. , Casey Francis X.M. , DeSutter Thomas M. 
Published: August 2016

Spills of brine wastewater produced during oil well drilling are occurring more frequently in the Great Plains, resulting in crop production loss on affected soil. Remediation requires removal of salt from the topsoil, which might be accomplished by leaching to subsurface horizons or subsurface drains. A laboratory study determined the effects of brine on saturated hydraulic conductivity (Ks) of four nonimpacted surface soils from western North Dakota, USA. Repacked soil cores were subjected to saturated water flow, followed by one pore volume of brine. Subsequent saturated water flow leached brine from the soil and reduced Ks as much as 97% (0.086–0.003 cm h−1) within 24 h. Effluent total dissolved solids (TDS) approached 250 000 mg L−1 then declined (5 mg L−1) with continued leaching, but Ks did not increase. Removal of soluble salts during leaching increased the relative sodium concentrations (ESP > 55), causing clay swelling/dispersion and reduced Ks. Postbrine gypsum application (11.2 Mg ha−1) to replace exchangeable sodium with calcium did not improve Ks. This evidence suggests that if subsurface drainage is used for reclaiming brine-impacted soils that special attention be given to where dispersion/swelling is occurring, leaching water quality, and closely positioning calcium amendments within the high sodium zones.
Ultrasonic attenuation anomalies of n-type superconductor Pr2−xCexCuO4
Authors: Chong Tet Vui , Abd-Shukor R. 
Published: August 2016

Pr[Formula: see text]Ce[Formula: see text]CuO4 ([Formula: see text] = 0 and 0.15) samples were prepared via solid state reaction. Pr2CuO4 sample is insulating while Pr[Formula: see text]Ce[Formula: see text]CuO4 sample exhibited an onset critical temperature, [Formula: see text] of 21 K. The temperature dependences of ultrasonic attenuation in these polycrystalline n-type superconductor Pr[Formula: see text]Ce[Formula: see text]CuO4 ([Formula: see text] = 0 and 0.15) samples have been measured between 80 K and 300 K using longitudinal and shear waves by pulse-echo-overlap method with frequencies between 5 MHz and 10 MHz. For the longitudinal mode, a pronounced attenuation peak around 200 K was observed in Pr2CuO4, but it is not observed in the superconducting material Pr[Formula: see text]Ce[Formula: see text]CuO4. For the shear mode, no pronounced attenuation peaks were observed in the samples. It is suggested that the attenuation peak in the [Formula: see text] = 0 sample at 200 K is due to the weak structural distortion induced by the magnetic transition.
Multi-cavity coupling acoustic metamaterials with low-frequency broad band gaps based on negative mass density
Authors: Yang Chuanhui , Wu Jiu Hui , Cao Songhua , Jing Li 
Published: August 2016

This paper studies a novel kind of low-frequency broadband acoustic metamaterials with small size based on the mechanisms of negative mass density and multi-cavity coupling. The structure consists of a closed resonant cavity and an open resonant cavity, which can be equivalent to a homogeneous medium with effective negative mass density in a certain frequency range by using the parameter inversion method. The negative mass density makes the anti-resonance area increased, which results in broadened band gaps greatly. Owing to the multi-cavity coupling mechanism, the local resonances of the lower frequency mainly occur in the closed cavity, while the local resonances of the higher frequency mainly in the open cavity. Upon the interaction between the negative mass density and the multi-cavity coupling, there exists two broad band gaps in the range of 0–1800 Hz, i.e. the first-order band gap from 195 Hz to 660 Hz with the bandwidth of 465 Hz and the second-order band gap from 1157 Hz to 1663 Hz with the bandwidth of 506 Hz. The acoustic metamaterials with small size presented in this paper could provide a new approach to reduce the low-frequency broadband noises.
Inhibition of the Biosynthesis of Prostaglandin E2 By Low-Dose Aspirin: Implications for Adenocarcinoma Metastasis
Authors: Boutaud Olivier , Sosa I. Romina , Amin Taneem , Oram Denise , Adler David , Hwang Hyun S. , Crews Brenda C. , Milne Ginger , Harris Bradford K. , Hoeksema Megan , Knollmann Bjorn C. , Lammers Philip E. , Marnett Lawrence J. , Massion Pierre P. , Oates John A. 
Published: August 2016

Abstract
Meta-analyses have demonstrated that low-dose aspirin reduces the risk of developing adenocarcinoma metastasis, and when colon cancer is detected during aspirin treatment, there is a remarkable 83% reduction in risk of metastasis. As platelets participate in the metastatic process, the antiplatelet action of low-dose aspirin likely contributes to its antimetastatic effect. Cycloxooxygenase-2 (COX-2)–derived prostaglandin E2 (PGE2) also contributes to metastasis, and we addressed the hypothesis that low-dose aspirin also inhibits PGE2 biosynthesis. We show that low-dose aspirin inhibits systemic PGE2 biosynthesis by 45% in healthy volunteers (P &lt; 0.0001). Aspirin is found to be more potent in colon adenocarcinoma cells than in the platelet, and in lung adenocarcinoma cells, its inhibition is equivalent to that in the platelet. Inhibition of COX by aspirin in colon cancer cells is in the context of the metastasis of colon cancer primarily to the liver, the organ exposed to the same high concentrations of aspirin as the platelet. We find that the interaction of activated platelets with lung adenocarcinoma cells upregulates COX-2 expression and PGE2 biosynthesis, and inhibition of platelet COX-1 by aspirin inhibits PGE2 production by the platelet–tumor cell aggregates. In conclusion, low-dose aspirin has a significant effect on extraplatelet cyclooxygenase and potently inhibits COX-2 in lung and colon adenocarcinoma cells. This supports a hypothesis that the remarkable prevention of metastasis from adenocarcinomas, and particularly from colon adenocarcinomas, by low-dose aspirin results from its effect on platelet COX-1 combined with inhibition of PGE2 biosynthesis in metastasizing tumor cells. Cancer Prev Res; 9(11); 855–65. ©2016 AACR.
Improved Climate Simulations through a Stochastic Parameterization of Ocean Eddies
Authors: Williams Paul D. , Howe Nicola J. , Gregory Jonathan M. , Smith Robin S. , Joshi Manoj M. 
Published: August 2016

Abstract
In climate simulations, the impacts of the subgrid scales on the resolved scales are conventionally represented using deterministic closure schemes, which assume that the impacts are uniquely determined by the resolved scales. Stochastic parameterization relaxes this assumption, by sampling the subgrid variability in a computationally inexpensive manner. This study shows that the simulated climatological state of the ocean is improved in many respects by implementing a simple stochastic parameterization of ocean eddies into a coupled atmosphere–ocean general circulation model. Simulations from a high-resolution, eddy-permitting ocean model are used to calculate the eddy statistics needed to inject realistic stochastic noise into a low-resolution, non-eddy-permitting version of the same model. A suite of four stochastic experiments is then run to test the sensitivity of the simulated climate to the noise definition by varying the noise amplitude and decorrelation time within reasonable limits. The addition of zero-mean noise to the ocean temperature tendency is found to have a nonzero effect on the mean climate. Specifically, in terms of the ocean temperature and salinity fields both at the surface and at depth, the noise reduces many of the biases in the low-resolution model and causes it to more closely resemble the high-resolution model. The variability of the strength of the global ocean thermohaline circulation is also improved. It is concluded that stochastic ocean perturbations can yield reductions in climate model error that are comparable to those obtained by refining the resolution, but without the increased computational cost. Therefore, stochastic parameterizations of ocean eddies have the potential to significantly improve climate simulations.