bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-cultured dermal-derived mesenchymal cells attenuate sepsis induced by cecal ligation and puncture in mice
Authors: Wang Yu , Tan Li , Jin Jie , Sun Huiqin , Chen Zelin , Tan Xu , Su Yongping , Shi Chunmeng 
Published: November 2015

AbstractSepsis remains a threat to critically ill patients and carries a high morbidity and mortality. Cell–based therapies have risen in prominence in recent years. Dermal-derived mesenchymal cells (DMCs) are attractive as one of the abundant sources from which to isolate mesenchymal cells for therapeutic applications and can be easily accessed with minimal harm to the donor. In this study, we described for the first time the use of non-cultured DMCs for treating sepsis in a cecal ligation and puncture (CLP) mouse model and investigated their immunomodulatory effects. We found that non-cultured DMCs administration provides a beneficial effect to improve survival in CLP-induced sepsis. This effect is partly mediated by the ability of DMCs to home to sites of injury, to reduce the inflammatory response, to inhibit apoptosis and to stimulate macrophage migration and phagocytosis. Our further findings suggest that DMCs treatment modulates the beneficial cytoprotective effects exhibited during sepsis, at least in part, by altering miRNA expression. These discoveries provide important evidence that non-cultured DMCs therapy has a specific anti-inflammatory effect on sepsis and provide the basis for the development of a new therapeutic strategy for managing clinical sepsis.
Distinctive effect on nerve growth factor-induced PC12 cell neurite outgrowth by two unique neolignan enantiomers from Illicium merrillianum
Authors: Tian Xinhui , Yue Rongcai , Zeng Huawu , Li Honglin , Shan Lei , He Weiwei , Shen Yunheng , Zhang Weidong 
Published: November 2015

AbstractMerrillianoid (1), a racemic neolignan possessing the characteristic benzo-2,7-dioxabicyclo[3.2.1]octane moiety, was isolated from the branches and leaves of Illicium merrillianum. Chiral separation of 1 gave two enantiomers (+)−1 and (−)−1. The structure of 1 was established by comprehensive spectroscopic analysis and single crystal X-ray diffraction. The absolute configurations of enantiomers were determined by quantum mechanical calculation. Compound (+)−1 exhibited a better neurotrophic activity than racemate 1 by promoting nerve growth factor (NGF) induced PC12 cell neurite outgrowth, while (−)−1 showed a distinctive inhibitory effect. Furthermore, a mechanism study indicated that the two enantiomers influenced NGF-induced neurite outgrowth of PC12 cells possibly by interacting with the trkA receptor and extracellular signal regulated kinases 1/2 (ERK1/2) and mitogen-activated protein kinase (MEK) in Ras/ERK signal cascade. But the phosphorylation level of serine/threonine kinase Akt1 and Akt2 in PI3K/Akt signal pathway showed no significant difference between (+)−1 and (−)−1.
Long-distance quantum information transfer with strong coupling hybrid solid system
Authors: Zhang Feng-Yang , Chen Xin-Yu , Li Chong , Song He-Shan 
Published: November 2015

AbstractIn this paper, we demonstrate how information can be transferred among the long-distance memory units in a hybrid solid architecture, which consists the nitrogen-vacancy (NV) ensemble acting as the memory unit, the LC circuit acting as the transmitter (receiver) and the flux qubit acting as the interface. Numerical simulation demonstrates that the high-fidelity quantum information transfer between memory unit and transmitter (receiver) can be implemented and this process is robust to both the LC circuit decay and NV ensemble spontaneous emission.
Majorana zero modes in the hopping-modulated one-dimensional p-wave superconducting model
Authors: Gao Yi , Zhou Tao , Huang Huaixiang , Huang Ran 
Published: November 2015

AbstractWe investigate the one-dimensional p-wave superconducting model with periodically modulated hopping and show that under time-reversal symmetry, the number of the Majorana zero modes (MZMs) strongly depends on the modulation period. If the modulation period is odd, there can be at most one MZM. However if the period is even, the number of the MZMs can be zero, one and two. In addition, the MZMs will disappear as the chemical potential varies. We derive the condition for the existence of the MZMs and show that the topological properties in this model are dramatically different from the one with periodically modulated potential.
Proteomic analysis of tylosin-resistant Mycoplasma gallisepticum reveals enzymatic activities associated with resistance
Authors: Xia Xi , Wu Congming , Cui Yaowen , Kang Mengjiao , Li Xiaowei , Ding Shuangyang , Shen Jianzhong 
Published: November 2015

AbstractMycoplasma gallisepticum is a significant pathogenic bacterium that infects poultry, causing chronic respiratory disease and sinusitis in chickens and turkeys, respectively. M. gallisepticum infection poses a substantial economic threat to the poultry industry and this threat is made worse by the emergence of antibiotic-resistant strains. The mechanisms of resistance are often difficult to determine; for example, little is known about antibiotic resistance of M. gallisepticum at the proteome level. In this study, we performed comparative proteomic analyses of an antibiotic (tylosin)-resistant M. gallisepticum mutant and a susceptible parent strain using a combination of two-dimensional differential gel electrophoresis and nano-liquid chromatography-quadrupole-time of flight mass spectrometry. Thirteen proteins were identified as differentially expressed in the resistant strain compared to the susceptible strain. Most of these proteins were related to catalytic activity, including catalysis that promotes the formylation of initiator tRNA and energy production. Elongation factors Tu and G were over-expressed in the resistant strains and this could promote the binding of tRNA to ribosomes and catalyze ribosomal translocation, the coordinated movement of tRNA and conformational changes in the ribosome. Taken together, our results indicate that M. gallisepticum develops resistance to tylosin by regulating associated enzymatic activities.
Hypouricemic and Nephroprotective Effects of Emodinol in Oxonate-Induced Hyperuricemic Mice are Mediated by Organic Ion Transporters and OIT3
Authors: Hui Wu , Yongliang Yuan , Yongde Chen , Guo Lu , Li Lan , Zhonglin Yang , Hui Ji , Qinghua Hu 
Published: November 2015

AbstractEmodinol, 1β,3β,23-trihydroxyolean-12-en-28-acid, as the main active ingredient firstly extracted from the rhizomes of Elaeagus pungens by our research group, has been demonstrated to exhibit uricosuric activity by our previous study. The aim of this study was to evaluate the uricosuric and nephroprotective effects of emodinol and explore its possible mechanisms in potassium oxonate-induced hyperuricemic mice with renal dysfunction. Mice were orally administrated 250 mg/kg of potassium oxonate once daily for 7 consecutive days to induce hyperuricemia with renal dysfunction. Emodinol was given at doses of 25, 50, and 100 mg/kg on the same day 1 h after oxonate treatment, and allopurinol (10 mg/kg) was given as a positive control. After 1 week, serum uric acid, serum creatinine, urine uric acid, urine creatinine, blood urea nitrogen, and hepatic xanthine oxidase activity were determined. The mRNA and protein levels of urate transporter 1, glucose transporter 9, ATP-binding cassette subfamily G member 2, organic anion transporter 1, oncoprotein-induced transcript 3, and organic cation/carnitine transporters in the kidney were detected by real-time polymerase chain reaction and Western blot analysis. In addition, urinary and renal Tamm-Horsfall glycoprotein concentrations were examined by ELISA assays. Emodinol significantly reduced serum urate levels, increased urinary urate levels and fractional excretion of uric acid, and inhibited hepatic xanthine oxidase activity in hyperuricemic mice. Moreover, potassium oxonate administration led to dys expressions of renal urate transporter 1, glucose transporter 9, ATP-binding cassette subfamily G member 2, organic anion transporter 1, and oncoprotein-induced transcript 3 as well as alternations of uromodulin concentrations, which could be reversed by emodinol. On the other hand, treatment of emodinol caused upregulated expressions of organic cation/carnitine transporters, resulting in an improvement of renal function characterized by decreased serum creatinine and blood urea nitrogen levels. Emodinol exhibited hypouricemic and nephroprotective actions by inhibiting xanthine oxidase activity and regulating renal ion transporters and oncoprotein-induced transcript 3, which may be a potential therapeutic agent in hyperuricemia and renal dysfunction.
Defining the Role of Cognitive Behavioral Therapy in Treating Chronic Low Back Pain: An Overview
Authors: Hanscom David A. , Brox Jens Ivar , Bunnage Ray 
Published: November 2015

Study Design Narrative review of the literature. Objectives Determine if the term cognitive behavioral therapy (CBT) is useful in clinical care and research. What literature supports these variables being relevant to the experience of chronic pain? What effects of CBT in treating these factors have been documented? What methods and platforms are available to administer CBT? Methods Chronic low back pain (CLBP) is a complex neurologic disorder with many components. CBT refers to a broad family of therapies that address both maladaptive thoughts and behaviors. There are several ways to deliver it. CLBP was broken into five categories that affect the perception of pain, and the literature was reviewed to see the effects of CBT on these variables. Results The term cognitive behavioral therapy has little use in future research because it covers such a wide range of therapies. CBT should always be defined by the problem it is intended to solve. The format and method of delivery should be defined because they have implications for outcomes. They are readily available even at the primary care level. The effectiveness of CBT is unquestioned regarding its effectiveness in treating each of the variables that affect CLBP. It is unclear why it is not more widely implemented. Conclusions CBT represents a family of therapies that are effective for a wide range of problems, many of which coexist with and influence CLBP. Each of the variables can be improved with focused CBT. Early, widespread adoption of CBT in treating and preventing CLBP is recommended. Future research and clinical care should focus on strategies to operationalize these well-documented treatments utilizing a public health approach.
Endpoint bounds for the bilinear Hilbert transform
Authors: Di Plinio Francesco , Thiele Christoph 
Published: November 2015

We study the behavior of the bilinear Hilbert transform

(

B
H
T

)

(\mathrm {BHT})

at the boundary of the known boundedness region

H

\mathcal H

. A sample of our results is the estimate

|

⟨

B
H
T

(

f
1

,

f
2

)
,

f
3

⟩

|

≤

C

|

F
1

|

3
4

|

F
2

|

3
4

|

F
3

|

−

1
2

log
⁡
log
⁡

(

e

e

+

|

F
3

|

min
{

|

F
1

|

,

|

F
2

|

}

)

,

\begin{equation*} |\langle \mathrm {BHT}(f_1,f_2),f_3 \rangle | \leq \textstyle C |F_1|^{\frac 34}|F_2| ^{\frac 34} |F_3|^{-\frac 12} \log \log \Big (\mathrm {e}^{\mathrm {e}} +\textstyle \frac {|F_3|}{\min \{|F_1|,|F_2|\}} \Big ),\end{equation*}

valid for all tuples of sets

F
j

⊂

R

F_j\subset \mathbb {R}

of finite measure and functions

f
j

f_j

such that

|

f
j

|

≤

1

F
j

|f_j| \leq \boldsymbol {1}_{F_j}

,

j
=
1
,
2
,
3

j=1,2,3

, with the additional restriction that

f
3

f_3

be supported on a major subset

F
3
′

F_3’

of

F
3

F_3

that depends on

{

F
j

:
j
=
1
,
2
,
3
}

\{F_j:j=1,2,3\}

. The use of subindicator functions in this fashion is standard in the given context. The double logarithmic term improves over the single logarithmic term obtained by D. Bilyk and L. Grafakos. Whether the double logarithmic term can be removed entirely, as is the case for the quartile operator, remains open.

We employ our endpoint results to describe the blow-up rate of weak-type and strong-type estimates for

B
H
T

\mathrm {BHT}

as the tuple

α
→

\vec \alpha

approaches the boundary of

H

\mathcal H

. We also discuss bounds on Lorentz-Orlicz spaces near

L

2
3

L^{\frac 23}

, improving on results of M. Carro et al. The main technical novelty in our article is an enhanced version of the multi-frequency Calderón-Zygmund decomposition.
Lack of Association between Blood Pressure Management by Anesthesia Residents and Competence Committee Evaluations or In-training Exam Performance
Authors: Sessler Daniel I. , Makarova Natalya , Riveros-Perez Ricardo , Brown David L. , Kimatian Stephen 
Published: November 2015

AbstractAbstract
In a cohort of 70 anesthesia residents, there was no association between five measures of blood pressure control obtained from electronic anesthesia records and either faculty evaluations of clinical competence or quantitative knowledge testing. While negative, this study provides a novel and important initial attempt to use electronic anesthesia records to evaluate the clinical performance.

Background
Prompt treatment of severe blood pressure instability requires both cognitive and technical skill. The ability to anticipate and respond to episodes of hemodynamic instability should improve with training. The authors tested the hypothesis that the duration of severe hypotension during anesthesia administered by residents correlates with concurrent adjusted overall performance evaluations by the Clinical Competence Committee and subsequent in-training exam scores.

Methods
The authors obtained data on 70 first- and second-year anesthesia residents at the Cleveland Clinic. Analysis was restricted to adults having noncardiac surgery with general anesthesia. Outcome variables were in-training exam scores and subjective evaluations of resident performance ranked in quintiles. The primary predictor was cumulative systolic arterial pressure less than 70 mmHg. Secondary predictors were administration of vasopressors, frequency of hypotension, average duration of hypotensive episodes, and blood pressure variability.

Results
The primary statistical approach was mixed-effects modeling, adjusted for potential confounders. The authors considered 15,216 anesthesia care episodes. A total of 1,807 hypotensive episodes were observed, lasting an average of 32 ± 20 min (SD) per 100 h of anesthesia, with 68% being followed by vasopressor administration. The duration of severe hypotension (systolic pressure less than 70 mmHg) was associated with neither Competence Committee evaluations nor in-training exam scores. There was also no association between secondary blood pressure predictors and either Competence Committee evaluations or in-training exam results.

Conclusions
There was no association between any of the five blood pressure management characteristics and either in-training exam scores or clinical competence evaluations. However, it remains possible that the measures of physiologic control, as assessed from electronic anesthesia records, evaluate useful but different aspects of anesthesiologist performance.
Liver Disease in Pediatric Patients With Ataxia Telangiectasia
Authors: Weiss Batia , Krauthammer Alexander , Soudack Michalle , Lahad Avishay , Sarouk Ifat , Somech Raz , Heimer Gali , Ben‐Zeev Bruria , Nissenkorn Andreea 
Published: November 2015

ABSTRACTObjective:Ataxia telangiectasia (A‐T) is a rare genetic multiorgan disease. Although gastrointestinal involvement is known, hepatic involvement in A‐T has not been investigated. We aimed to study the hepatic involvement in a large cohort of patients with A‐T.Methods:A retrospective review of patients, studied from January 1986 to January 2015 at a National A‐T Center. Clinical data including demographic, genetic, laboratory, nutritional, radiographic, and histological data were retrieved.Results:Fifty‐three patients, 27 (49%) boys, age 14.6 ± 5.2 years (range 5.9–26.1 years), were included. Twenty‐three patients (43.4%), age 9.9 ± 5.1 years, had consistently abnormal liver enzymes. The mean enzyme levels were alanine aminotransferase 76.8 ± 73.8 IU/L, aspartate aminotransferase 70 ± 50 IU/L, alkaline phosphatase 331 ± 134 IU/L, and gamma glutamyl transferase 114.7 ± 8 IU/L. Evaluation of other etiology of liver disease was negative. Ultrasonography revealed fatty liver in 9 of them (39%). Liver biopsy was performed in 2 patients, revealing mild‐to‐moderate steatosis in both, and fibrosis in 1 patient. Progression to advanced liver disease occurred in 2 of 23 (9%) patients within 2 to 5 years. Dyslipidemia was significantly associated with abnormal liver enzymes: 3 of 30 (10%) patients without abnormal liver enzymes versus 10 of 23 (45.5%) patients with abnormal liver enzymes, respectively (P < 0.05, Fisher exact test). No correlation was found between hepatic involvement and HbA1C, sex, presence of malignancy, or type of mutation.Conclusions:Abnormal liver enzymes and fatty liver are common in patients with A‐T and may progress to advanced liver disease at a young age. These findings are novel and implicate that patients with A‐T with abnormal liver enzymes should be evaluated for the presence of liver disease.
Home‐Based Screening for Biliary Atresia Using Infant Stool Color Cards in Canada
Authors: Morinville Véronique , Ahmed Najma , Ibberson Cindy , Kovacs Lajos , Kaczorowski Janusz , Bryan Stirling , Collet Jean‐Paul , Schreiber Richard 
Published: November 2015

ABSTRACTObjectives:Biliary atresia (BA) is a leading cause of liver failure and liver transplantation in pediatrics. BA manifests by 3 weeks of life with jaundice and pale stools. Delayed diagnosis and surgical intervention with Kasai portoenterostomy after 3 months of age is significantly associated with poor prognosis for native liver survival. A national Taiwan infant stool color card (SCC) screening program has entirely eliminated late Kasai portoenterostomy >90 days of age and improved native liver survival. A recent large‐scale prospective cohort study in British Columbia, Canada, indicated that distribution of SCC on the maternity ward was feasible, led to high utilization rate, and was cost‐effective. The aim of the present study was to assess the generalizability of this screening strategy in another Canadian jurisdiction with a different sociodemographic profile.Methods:An SCC was distributed to families of newborns discharged at St Mary's Hospital Center, Montreal, Quebec. Families were instructed to monitor their infant's stool color for 21 days and then complete and mail the SCC to the study center. Phone surveys to families who did not return cards were used to estimate total card utilization rate.Results:Two thousand two hundred forty‐six infants were eligible for inclusion; 99.9% were enrolled. Mail SCC return rate was 63.3%. No cases of BA were identified. All of the 118 families who completed the phone survey reported that they had utilized the SCC. Conservative and optimistic estimates for total card utilization rates were 82% and 100%, respectively.Conclusions:The high enrollment and utilization rates in this screening study strongly support the feasibility of implementing a Canadian SCC screening program to improve outcomes of children with BA.
DISC1 regulates astrogenesis in the embryonic brain via modulation of RAS/MEK/ERK signaling through RASSF7
Authors: Wang Shukun , Liang Qingli , Qiao Huimin , Li Hong , Shen Tianjin , Ji Fen , Jiao Jianwei 
Published: November 2015

ABSTRACTDisrupted in Schizophrenia1 (DISC1) is known as a high susceptibility gene of schizophrenia. More recent studies have connected schizophrenia with glia defects and dysfunction. However, it is unclear whether there is connection between DISC1 and gliogenesis defect. Thus, a precise understanding of DISC1 (a ubiquitously expressed brain protein) on astrogenesis in the late stage of embryonic mouse brain development needs to be deeply investigated. Here, we show that suppression of DISC1 expression represses astrogenesisin vitroandin vivo, whereas DISC1 overexpression substantially enhances the process. Furthermore, mouse and human DISC1 overexpression rescued astrogenesis defects caused by DISC1 knowndown. Mechanistically, DISC1 activates downstream RAS/MEK/ERK signaling pathway via directly associating with the C terminal domain of RASSF7, a RAS association protein. Also, the pERK complex undergoes nuclear translocation and influences the expression of genes related to astrogenesis. Briefly, our results demonstrate that DISC1 regulates astrogenesis by modulating RAS/MEK/ERK signaling via RASSF7 and provide a framework for understanding how DISC1 dysfunction leads to neuropsychiatric diseases.
In plantaexpression screens of candidate effector proteins from the wheat yellow rust fungus reveal processing bodies as a pathogen-targeted plant cell compartment
Authors: Petre Benjamin , Saunders Diane G.O. , Sklenar Jan , Lorrain Cécile , Krasileva Ksenia V. , Win Joe , Duplessis Sébastien , Kamoun Sophien 
Published: November 2015

ABSTRACTRust fungal pathogens of wheat (Triticumspp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal effectors in the model plantNicotiana benthamiana. This pipeline involves selecting candidate effectorsin silicoand performing cell biology and protein-protein interaction assaysin plantato gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogenPuccinia striiformisf sptritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) fromN. benthamianaand wheat. Our work identifies P-bodies as a novel plant cell compartment targeted by pathogen effectors.