bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and preliminary characterization of human MICU2
Authors: Li Dan , Wu Wenping , Pei Hairun , Wei Qiang , Yang Qingzhan , Zheng Jimin , Jia Zongchao 
Published: June 2016

ABSTRACT
MICU2 has been reported to interact with MICU1 and participate in the regulation of mitochondrial Ca2+ uptake, although the molecular determinants underlying the function of MICU2 is unknown. In order to characterize MICU2 we screened a series of N-terminal and C-terminal truncations and obtained constructs which can be expressed in abundance, giving rise to soluble samples to enable subsequent characterizations. Size exclusion chromatography (SEC) and multi-angle laser light scattering (MALLS) revealed that MICU2 exists as a monomer in Ca2+-free conditions but forms a dimer in Ca2+-bound conditions. Unlike MICU1, the C-helix domain of MICU2 exhibits no influence on protein conformation in both Ca2+-free and Ca2+-bound forms. Furthermore, mutation of the first EF-hand abolishes the ability of MICU2 to switch to a dimer in the presence of Ca2+, indicating that the first EF-hand is not only involved in Ca2+ binding but also in conformational change. Our pull-down and co-immunoprecipitation assays suggest that, in addition to disulfide bonds, salt bridges also contribute to MICU1-MICU2 heterodimer formation.
Differences in cardiovascular toxicities associated with cigarette smoking and snuff use revealed using novel zebrafish models
Authors: Folkesson Maggie , Sadowska Natalia , Vikingsson Svante , Karlsson Matts , Carlhäll Carl-Johan , Länne Toste , Wågsäter Dick , Jensen Lasse 
Published: June 2016

ABSTRACT
Tobacco use is strongly associated with cardiovascular disease and the only avoidable risk factor associated with development of aortic aneurysm. While smoking is the most common form of tobacco use, snuff and other oral tobacco products are gaining popularity, but research on potentially toxic effects of oral tobacco use has not kept pace with the increase in its use. Here, we demonstrate that cigarette smoke and snuff extracts are highly toxic to developing zebrafish embryos. Exposure to such extracts led to a palette of toxic effects including early embryonic mortality, developmental delay, cerebral hemorrhages, defects in lymphatics development and ventricular function, and aneurysm development. Both cigarette smoke and snuff were more toxic than pure nicotine, indicating that other compounds in these products are also associated with toxicity. While some toxicities were found following exposure to both types of tobacco product, other toxicities, including developmental delay and aneurysm development, were specifically observed in the snuff extract group, whereas cerebral hemorrhages were only found in the group exposed to cigarette smoke extract. These findings deepen our understanding of the pathogenic effects of cigarette smoking and snuff use on the cardiovascular system and illustrate the benefits of using zebrafish to study mechanisms involved in aneurysm development.
SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes
Authors: Krueger Felix , Andrews Simon R. 
Published: June 2016

Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP) positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base ’N’ and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data.
Strategic Planning of Innovative Development As the Basis for the Economic Growth of the Russian Federation
Authors: Горохова  , Gorokhova I. , Парфенова  , Parfenova E. 
Published: June 2016

The article discusses the current state of the Russian economy and its innovative
development, as in recent years, very relevant was the question of increasing
economic growth by increasing the volume of production and extraction of fuel
and energy resources, and through the implementation of innovative potential
of the country. To encourage this trend in 2011 approved the Strategy of innovative
development of Russia for the period up to 2020. The article analyses the results
and the conclusions that have already created the infrastructure of the national
innovation system, established and developed the implementation of the
innovative development Program (IDP). As a result of writing problems faced
by state-owned companies in the development of IDP.
Toward a Greater Understanding: Utilizing Book Discussions to Effectively Engage Students in the Exploration of Women and Leadership Issues
Authors: Scott Heather Inez Ricks 
Published: June 2016

This article examines the use of a book discussion as an instructional tool for developing leadership competency skills in female university students. A book discussion centered on Sheryl Sandberg’s book Lean In was held as a means to conceptualize discourse regarding leadership issues in the arena of women and leadership in a multidisciplinary campus wide symposium. In an effort to assess the effectiveness of such a program to learn about leadership issues, student commentary was collected during the discussion via an audio recording device.
A qualitative exploration of the resulting commentary focused on this initiative as an effort to provide insight into the efficacy of book discussions as a best practice for facilitating the engagement of students in the exploration of leadership issues. As leadership educators seek to develop pedagogical tools that catalyze transformative learning, research regarding tools and methods by which faculty equip students to explore leadership becomes increasingly critical.
Systemic Influences on Optimal Equity-Credit Investment
Authors: Capponi Agostino , Frei Christoph 
Published: June 2016

We introduce an equity-credit portfolio framework taking into account the structural interaction of market and credit risk, along with their systemic dependencies. We derive an explicit expression for the optimal investment strategy in stocks and credit default swaps (CDSs). We exploit its representation structure and analyze the mechanisms driving the optimal investment decisions. The transmission of market risk premia is the key mechanism through which systemic influences affect the optimal investment strategy. We develop a novel calibration procedure and find that systemic dependencies are statistically significant when the model is fitted to historical time series of equity and CDS data. An empirical analysis with data of companies in the Dow Jones Industrial Average 30 reveals the critical role of systemic risk in portfolio monitoring. This paper was accepted by Noah Gans, stochastic models and simulation.
Shrinkage and Static Bending Properties of Juniperus scopulorum from the Rocky Mountains
Authors: Vaughan D. R. , Mackes K. H 
Published: June 2016

Abstract

Juniperus scopulorum is a common tree in the lower elevations of the Rocky Mountains, and is often removed in restoration treatments. This work reports on selected mechanical and physical properties of J. scopulorum. Ten trees were felled and processed, and a static bending machine was used to test 162 specimens. Additionally, 82 ovendried specimens were assessed for shrinkage and density. Results show an air-dry modulus of elasticity (MOE) of 4,611 MPa, air-dry modulus of rupture (MOR) of 68,460 kPa, green MOE of 3,846 MPa, green MOR of 46,760 kPa, specific gravity of 0.4184 (green basis), and shrinkage value of 7.956 percent. These numbers suggest several new uses for the wood including highway signage, guardrails, and bioenergy feedstock.
Physical education and sports students’ attitudes towards inclusive education (The sample of Kocaeli University)
Authors: Aydın Mensure , İra Nejat , Meriç Bingül Bergün , Bulgan Çiğdem 
Published: June 2016

The aim of this study was to observe if Physical Education and Sports students’ attitudes towards Inclusive Education differ or not. The research was done according to the scanning/screening model. “Personal information form” (2004) - (reformed by Orel and his friends) and “Attitude Scale related to Inclusion” (1995)-(reformed by Antonak & Larivee and adapted into Turkish by Kırcaali & Iftar ) were used for Kocaeli University School of Physical Education and Sports /Physical Education and Sports Department’s 1st grade and the 2nd grade students (121 students) who didn’t take the Inclusive Education class and the 3rd and 4th grade students (193 students) who took the Inclusive Education class. In the statistical analysis of the research, frequency values of the data (which were obtained from personal information forms) were surveyed and while analysing the data (obtained from the attitude scale related to inclusion) arithmetic average, standard deviation and t test (for the difference between two groups ) were applied.In statistical analysis and in all parameters numerical difference was seen on the side of the students who took inclusive education class but no statEistically significant difference was found . (P<0,05). As a result, it can be said that Inclusive Education class had a positive change on the attitudes of Physical Education teacher candidates towards inclusion.
Effect of D Genome on Wheat Anther Culture Response After Cold and Mannitol Pretreatment
Authors: Lazaridou Theano , Pankou Chryssanthi , Xynias Ioannis , Roupakias Demetrios 
Published: June 2016

AbstractThe present study was conducted to determine the effect of the D genome on embryoid induction and green plant regeneration in wheat anther culture and how it is influenced by low temperature and mannitol treatment. For this reason, the anther culture response of two Canadian bread wheat cultivars and their extracted tetraploids (AABB) was studied. As controls two cultivars well responding to anther-culture (i.e. cvs. Kavkaz/Cgn and Acheron) and a no-responding cultivar (cv. Vergina) were used. Approximately 3000 anthers of these cultivars were cultured and three pre-treatments were applied: cold pre-treatment for 7 and 18 days at 4°C, and 0.3M mannitol for seven days at 4°C. W14 and 190-2 were used as induction and regeneration media, respectively, and the basic MS medium as the rooting medium. No green plants were produced from the tetraploids, which supports the view that the D-genome chromosomes are necessary for androgenic response in wheat. Furthermore, the Canadian cultivars performed better after 18-day pre-treatment at 4°C. The extracted tetraploids produced fewer embryoids and performed better after seven days of cold pre-treatment. The controls well responding to anther culture performed better than the Canadian cultivars, although their best response was recorded after seven-day cold pre-treatment. Cultivar Vergina produced no green plants. The presence of mannitol influenced negatively both embryoid and green plant production. It was concluded that the D genome plays a crucial role in anther culture response of wheat and that this response is influenced by both the genotype and the duration of cold pre-treatment.
Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity
Authors: Kikowska Małgorzata , Thiem Barbara , Sliwinska Elwira , Rewers Monika , Kowalczyk Mariusz , Stochmal Anna , Długaszewska Jolanta 
Published: June 2016

AbstractAn efficient micropropagation protocol for production of genetically uniform clones ofEryngium campestreL. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro- and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg l−1benzyladenine (BA) and 0.1 mg l−1indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteriaStaphylococcus aureus, as well as dermatophytesTrichophyton mentagrophytesandT. rubrum.
Palynofacies patterns of the Highveld coal deposits (Karoo Basin, South Africa): Clues to reconstruction of palaeoenvironment and palaeoclimate
Authors: Wheeler Alexander , Götz Annette E. 
Published: June 2016

Abstract The early Permian represents a crucial period of climate change in Gondwana. This climate signature is captured in the palynological record that represents the floral assemblage of the region. Palynofacies analysis of the No. 2 Coal Seam of the Highveld Coalfield provides a high-resolution picture of this climatic shift, as well as detailing the vegetation patterns and local environments. Core samples taken from two localities were studied with respect to the characteristics of the plant debris and the palynomorph assemblages to differentiate between regional and local signatures. At both of the sampling localities, the No. 2 Coal Seam is split into a Lower Coal Seam and an Upper Coal Seam by a siltstone and a sandstone intraseam parting, respectively. The uneven palaeotopography and distal depositional environment of the Highveld Coalfield distinguish it from the northern Witbank Coalfield as a river-dominated delta plain, with differences in the palaeoenvironment at each locality. Results from the Lower Coal Seam indicate a fern-dominated lowland and conifer-dominated upland. This gives way to a Glossopteris-dominated lowland and a diverse gymnospermous assemblage in the upland of the Upper Coal Seam. This change in floral composition is also observed in the adjacent Witbank Coalfield and is likely caused by climate amelioration related to the movement of Gondwana away from the South Pole.
Multi objective Flower Pollination Algorithm for solving capacitor placement in radial distribution system using data structure load flow analysis
Authors: Tamilselvan V. , Jayabarathi T. 
Published: June 2016

Abstract
The radial distribution system is a rugged system, it is also the most commonly used system, which suffers by loss and low voltage at the end bus. This loss can be reduced by the use of a capacitor in the system, which injects reactive current and also improves the voltage magnitude in the buses. The real power loss in the distribution line is the I2R loss which depends on the current and resistance. The connection of the capacitor in the bus reduces the reactive current and losses. The loss reduction is equal to the increase in generation, necessary for the electric power provided by firms. For consumers, the quality of power supply depends on the voltage magnitude level, which is also considered and hence the objective of the problem becomes the multi objective of loss minimization and the minimization of voltage deviation. In this paper, the optimal location and size of the capacitor is found using a new computational intelligent algorithm called Flower Pollination Algorithm (FPA). To calculate the power flow and losses in the system, novel data structure load flow is introduced. In this, each bus is considered as a node with bus associated data. Links between the nodes are distribution lines and their own resistance and reactance. To validate the developed FPA solutions standard test cases, IEEE 33 and IEEE 69 radial distribution systems are considered.
One phase active filter with energy storage for active power surge compensation in feed line
Authors: Sowa Kacper , Baszyński Marcin , Piróg Stanisław 
Published: June 2016

Abstract
The paper presents a concept of an active filter with energy storage. This solution can be used for the compensation of momentary one phase high power loads with discontinued power consumption (e.g. spot welding machines). Apart from the typical filtering capabilities, the system’s task is also the continuity of the input power from the feeder line and limiting its fluctuation. The proposed by the author’s solution can produce measurable economic benefits by reducing the rated power necessary to energize periodically operating loads and improving the indicators of electrical energy quality. The developed method of active power surges compensation enables a flexible approach to requirements concerning the rated power of the point to which the periodically operating loads with high peak current value are connected. The tests were conducted on a simulation model specially developed in Matlab & Simulink environment, proving high effectiveness of the presented solution.
Generalized space vector control for current source inverters and rectifiers
Authors: Roseline J. Anitha , Kumaran M. Senthil , Rajini V. 
Published: June 2016

Abstract
Current source inverters (CSI) is one of the widely used converter topology in medium voltage drive applications due to its simplicity, motor friendly waveforms and reliable short circuit protection. The current source inverters are usually fed by controlled current source rectifiers (CSR) with a large inductor to provide a constant supply current. A generalized control applicable for both CSI and CSR and their extension namely current source multilevel inverters (CSMLI) are dealt in this paper. As space vector pulse width modulation (SVPWM) features the advantages of flexible control, faster dynamic response, better DC utilization and easy digital implementation it is considered for this work. This paper generalizes SVPWM that could be applied for CSI, CSR and CSMLI. The intense computation involved in framing a generalized space vector control are discussed in detail. The algorithm includes determination of band, region, subregions and vectors. The algorithm is validated by simulation using MATLAB /SIMULINK for CSR 5, 7, 13 level CSMLI and for CSR fed CSI.
The influence of impurities on the operation of selected fuel ignition systems in combustion engines
Authors: Różowicz Sebastian , Tofil Szymon 
Published: June 2016

Abstract
The paper attempts to determine the impact of fuel impurities on the spark discharge energy and the wear of the spark plug electrode. Spark plugs were analyzed in two typical configurations of the ignition system. A number of tests were conducted to determine the wear of the spark plug electrode exposed to different types of impurities. The spark discharge energy for new and worn spark plugs was determined through calculation.
Low-dose intravenous lipid emulsion for the treatment of severe quetiapine and citalopram poisoning
Authors: Purg Darinka , Markota Andrej , Grenc Damjan , Sinkovič Andreja 
Published: June 2016

AbstractThe treatment of quetiapine and/or citalopram poisoning is mainly supportive and involves gastric lavage, activated charcoal, intubation, and mechanical ventilation. Recently, however, there were reports of successful treatment with intravenous lipid emulsion. Here we report a case of a 19-year-old Caucasian girl who ingested approximately 6000 mg of quetiapine, 400 mg of citalopram, and 45 mg of bromazepam in a suicide attempt. The patient developed ventricular tachycardia and epileptic seizures 12 h after admission to the hospital. As the patient’s condition deteriorated, we combined standard therapy (intubation, mechanical ventilation, and vasopressors) with low-dose intravenous lipid emulsion (ILE) (a total of 300 mL of 20 % lipid emulsion) and normalised her heart rhythm and stopped the seizures. She was discharged to the psychiatric ward after 48 h and home after a prolonged (2-month) psychiatric rehabilitation. Intravenous lipid emulsion turned out to be effective even in the lower dose range than previously reported for quetiapine poisoning in patients presenting with seizure and ventricular arrhythmia. To our knowledge, there are no case reports describing the use of ILE in treating citalopram poisoning.
The relationship between facial morphology, body measurements and socio-economic factors
Authors: Jandová Marie , Urbanová Petra 
Published: June 2016

Background and aim: The effect of socio-economic factors (living conditions) and parental smoking habits on development of facial morphology and body measurements was studied on a longitudinal Czech sample of 25 girls and 25 boys.
Subjects and methods: A set of studied digitalized photographs taken from 0.5 to 18 years in intervals of 6 months originated in the Brno Longitudinal Study. Facial shape changes of sub-adult participants were described using a configuration of 27 landmarks and further studied by using methods of geometric morphometric and multivariate statistics. In order to localize growth-related shape changes within the face, the studied region was divided into upper, middle and lower facial units and analyzed separately.
Results and conclusion: The results show that in the course of ontogenesis there is a strong correlation between facial shape change and body measurements, height included (r=0.10 and r=0.24 in boys and in girls, respectively). The pubertal spurt of the facial shape change rate was revealed at 10.5 years in girls and at 11.5 years in boys. The earlier onset of the pubertal rate increase in facial shape changes in boys was associated with records of poor living conditions. In addition, the mother’s smoking habits were linked to a noticeable facial shape change.
The Effect of Adding Hesperidin, Diosmin, Quercetin and Resveratrol Extracts to Feed for Turkey Hens on Selected Immunological and Biochemical Blood Indices
Authors: Ognik Katarzyna , Cholewińska Ewelina , Czech Anna 
Published: June 2016

Abstract
It was postulated that naturally occurring phenolic compounds obtained from various plant species may have potential use as feed additives for poultry. Therefore the aim of the study was to compare extracts of hesperidin, diosmin, quercetin and resveratrol in terms of their health-promoting (particularly immunostimulatory) effect on turkeys at different ages. The experiment was conducted on 720 Big 6 turkey hens assigned to 6 experimental groups of 120 individuals (6 repetitions with 20 birds each). The turkey hens in group G-C were the control, receiving a basal compound feed with no experimental additives. The turkey hens in the remaining groups, from the first to the 16th week of life, received a basal diet containing hesperidin (group G-H), diosmin (group G-D), quercetin (group G-Q) or resveratrol (group G-R) in the amount of 200 g per tonne of feed. Ht, Hb, RBC, WBC, lysozyme activity, %PC, IgA, IL-6, GLU, TP and minerals were determined in blood samples. The addition of quercetin or resveratrol in the amount of 200 g per tonne of feed was found to have a beneficial effect on haemoglobin synthesis and phosphorus availability, and may also modulate immunity in turkey hens.