bluuebunny/crossref_metadata_2025_split huggingface dataset:
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge
Authors: Wang Xiang , Fu Weihui , Yuan Songhua , Yang Xi , Song Yufeng , Liu Lulu , Chi Yudan , Cheng Tao , Xing Man , Zhang Yan , Zhang Chao , Yang Yong , Zhu Caihong , Zhang Xiaoyan , Xiong Sidong , Xu Jianqing , Zhou Dongming 
Published: May 2017

AbstractSince 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus-based vaccine, AdC68-H7HA, by cloning the H7N9 haemagglutinin (HA) gene into the chimpanzee adenoviral vector AdC68. The efficacy of AdC68-H7HA was evaluated in mice as well as guinea pigs. For comparison, an H7N9 DNA vaccine based on HA was also generated and tested in mice and guinea pigs. The results demonstrated that both AdC68-H7HA and the DNA vaccine prime-adenovirus boost regimen induced potent immune responses in animals and completely protected mice from lethal H7N9 influenza viral challenge. A post-immunization serum transfer experiment showed that antibody responses could completely protect against lethal challenge, while a T cell depletion experiment indicated that HA-specific CD8+T cells responses also contributed to protection. Therefore, both HA-specific humoral immunity and cellular immunity play important roles in the protection. These data suggest that the chimpanzee adenovirus expressing HA is a promising vaccine candidate for H7N9 virus or other influenza viral subtypes.
Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
Authors: Ding Yanjun , Li Xingmei , Guo Yadong , Duan Weicheng , Ling Jiang , Zha Lagabaiyla , Yan Jie , Zou Ying , Cai Jifeng 
Published: May 2017

AbstractEstimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K+) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K+ions. The linear range for K+ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K+ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K+ions concentration: PMI(h) = −0.55 + 1.66 × CK+(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K+, which may be potentially a better way for estimation of PMI in medico-legal practice.
Hotspot analyses indicate significant conservation gaps for evergreen broadleaved woody plants in China
Authors: Xu Yue , Shen Zehao , Ying Lingxiao , Wang Zhiheng , Huang Jihong , Zang Runguo , Jiang Youxu 
Published: May 2017

AbstractEvergreen broadleaved woody plants (EBWPs) are dominant components in forests and savanna of the global tropic and subtropic regions. Southern China possesses the largest continuous area of subtropical EBWPs distribution, harboring a high proportion of endemic species. Hotspot and gap analyses are effective methods for analyzing the spatial pattern of biodiversity and conservation and were used here for EBWPs in China. Based on a distribution data set of 6,265 EBWPs with a spatial resolution of 50 × 50 km, we measured diversity of EBWPs in China using four indices: species richness, corrected weighted endemism, relative phylogenetic diversity, and phylogenetic endemism. According to the results based on 10% threshold, 15.73% of China’s land area was identified as hotspots using at least one diversity index. Only 2.14% of China’s land area was identified as hotspots for EBWPs by all four metrics simultaneously. Most of the hotspots locate in southern mountains. Moreover, we found substantial conservation gaps for Chinese EBWPs. Only 25.43% of the hotspots are covered by existing nature reserves by more than 10% of their area. We suggest to promote the establishment and management of nature reserve system within the hotspot gaps.
Image noise reduction based on applying adaptive thresholding onto PDEs methods
Authors: Yahya Ali Abdullah , Tan Jieqing , Su Benyu , Liu Kui , Hadi Ali Naser 
Published: May 2017

In this study the authors present a novel image denoising method based on applying adaptive thresholding on partial differential (PDEs) methods. In the proposed method the authors utilise the adaptive thresholding to blend the total variation filter with anisotropic diffusion filter. The adaptive thresholding has a high capacity to adapt and change according to the amount of noise. More specifically, applying a hard thresholding on the higher noise areas, whereas, applying soft thresholding on the lower noise areas. Therefore, the authors can successfully remove the noise effectively and maintain the edges of the image simultaneously. Based on the adaptation and stability of the adaptive thresholding we can achieve; optimal noise reduction and sharp edges as well. Experimental results demonstrate that the new algorithm consistently outperforms other reference methods in terms of noise removal and edges preservation, in addition to 4.7 dB gain higher than those in the other reference algorithms.
The Clinical Relevance of Noncriteria Antiphospholipid Antibodies
Authors: Sanna Giovanni , Bertolaccini Maria 
Published: May 2017

AbstractWhile lupus anticoagulant (LA), anticardiolipin antibodies (aCL), anti-β2 glycoprotein I (anti-β2GPI) antibodies represent the best available and the most widely used tests in the investigation for antiphospholipid syndrome (APS), evidence gathered in recent years indicates that other antiphospholipid antibodies (aPL) specificities may also play a role in the syndrome. Several autoantibodies have been shown to be complexed with phospholipids other than cardiolipin, or to some domains of β2GPI, or else directed to other proteins of the coagulation cascade, and these have also been proposed to be of relevance to APS, and their diagnostic value and clinical utility are the focus of current research.
Laboratory Diagnostics of Antiphospholipid Syndrome
Authors: Bison Elisa , Denas Gentian , Jose Seena , Zoppellaro Giacomo , Banzato Alessandra , Pengo Vittorio 
Published: May 2017

AbstractDiagnosis of antiphospholipid syndrome (APS) lies in the recognition of antiphospholipid antibodies (aPL). As standardization of tests for the detection of aPL is far from being optimal and reference material is not available, inappropriate diagnoses of APS are not unusual. In the last few years, the concept of triple test positivity has emerged as a useful tool to identify patients with APS. Clinical studies on patients and carriers of triple positivity clearly show that these individuals are at high risk of thromboembolic events and pregnancy loss. Moreover, triple positivity arises from a single (probably pathogenic) antibody directed to domain 1 of β2-glycoprotein I, a protein whose function is still unknown. Studies on homogenous group of patients with single or double positivity are scant, and uncertainties arise on their association with clinical events. Promising but undetermined results come also from the determination of antibodies directed to phosphatidylserine/prothrombin complex. Interpretation of laboratory profile in APS is challenging, and the collaboration between clinical pathologists and clinicians is highly desirable.
The Genetic Approach: Next-Generation Sequencing-Based Diagnosis of Congenital and Infantile Myopathies/Muscle Dystrophies
Authors: Rost Simone , Kolokotronis Konstantin , Meng Gerhard , Pluta Natalie , Müller-Reible Clemens , Kress Wolfram 
Published: May 2017

AbstractThe practical basis for massive parallel sequencing is described to help clinicians in choosing the most adequate diagnostic approach for childhood myopathies. The key quality feature for massive parallel sequencing is the sequence depth (coverage) as a prerequisite for variant identification and quantification of sequence copy numbers. Our experience with a next-generation sequencing gene panel for the analysis of muscular dystrophies/myopathies with infantile or juvenile onset resulted in the identification of pathogenic or likely pathogenic mutations in approximately 41% (of 141 patients), thus leading to a definitive diagnosis. A subset of patients shows an accumulation of “excess” heterozygous variants that may act as modifiers of the phenotype. Massive parallel sequencing has become a reliable and cost-effective method, but it requires exact clinical, bioptic, and/or radiologic information to evaluate the clinical relevance of possibly pathologic variants.
Association of Step Width with Accelerated Sprinting Performance and Ground Reaction Force
Authors: Nagahara Ryu , Mizutani Mirai , Matsuo Akifumi , Kanehisa Hiroaki , Fukunaga Tetsuo 
Published: May 2017

AbstractThis study aimed to describe changes in step width (SW) during accelerated sprinting, and to clarify the relationship of SW with sprinting performance and ground reaction forces. 17 male athletes performed maximal-effort 60 m sprints. The SW and other spatiotemporal variables, as well as ground reaction impulses, over a 52 m distance were calculated. Average values for each 4 steps during acceleration were calculated to examine relationships among variables in different sections. The SW rapidly decreased up to the 13th step and slightly afterward during accelerated sprinting, showing a bilinear phase profile. The ratio of SW to the stature was significantly correlated with running speed based on average values over the 52 m distance and in the 9th–12th step section during accelerated sprinting. The SW ratio positively correlated with medial, lateral and mediolateral impulses in all step sections, except for medial impulse in the 17th–20th step section. These results indicate the importance of wider SW for better sprinting performance, especially in the 9th–12th step section. Moreover, the wider SW was associated with larger medial impulse and smaller lateral impulse, suggesting that a wide SW contributes to the production of greater mediolateral body velocity during accelerated sprinting.
Brain connectivity dynamics during social interaction reflect social network structure
Authors: Schmälzle Ralf , Brook O’Donnell Matthew , Garcia Javier O. , Cascio Christopher N. , Bayer Joseph , Bassett Danielle S. , Vettel Jean M. , Falk Emily B. 
Published: May 2017

Significance
We examine brain dynamics during a common social experience—social exclusion—to determine whether cohesive networks in the brain support navigation of the social world and contribute to the shape of friendship networks. Specifically, exclusion is associated with increased cohesion within brain networks that support understanding what other people think and feel. Furthermore, using social network analysis, we find that variability in brain dynamics is associated with the shape of participants’ friendship networks. Bringing together findings related to brain network dynamics and social network dynamics illuminates ways that psychological processes may shape and be shaped by social environments.
Molecular codes for cell type specification in Brn3 retinal ganglion cells
Authors: Sajgo Szilard , Ghinia Miruna Georgiana , Brooks Matthew , Kretschmer Friedrich , Chuang Katherine , Hiriyanna Suja , Wu Zhijian , Popescu Octavian , Badea Tudor Constantin 
Published: May 2017

Significance
We report here transcriptome analysis by RNA sequencing (RNASeq) of genetically labeled and affinity-purified mouse retinal ganglion cell (RGC) populations. Using a previously established conditional knock-in reporter strategy, we label RGCs from which specific transcription factors have been removed and determine the consequences on transcriptional programs at different stages critical to RGC development. We find that Brn3b and Brn3a control only small subsets of Brn3–RGC–specific transcripts. We identify extensive combinatorial sets of RGC transcription factors and cell surface molecules and show that several RGC-specific genes can induce neurite-like processes cell autonomously in a heterologous system.
Populist discourses in the Hungarian public sphere
Authors: Barát Erzsébet 
Published: May 2017

AbstractExtendingLaclau’s (2005)claim to the importance of ‘populism’ for a democratic politics, I argue that the ‘we’, when issued from a left-wing position around ‘empty signifiers’, must be informed by an inclusionary logic. In my critical study of the Hungarian Government’s right-wing populist discourses, I show that their billboard campaign in 2016 against the European Union’s migrant quota articulates the ‘we are not like them’ exclusionary distinction of ‘against and over’. Through Judith Butler’s category of vulnerability and Mary Matsuda’s relative distinction between immediate and indirect targets of exclusion, I explore the inclusionary logic of the campaign by the Two-Tailed Dog Party. I demonstrate that a non-identitarian collective subject "from below" in their alternative left-wing populism is made possible by the power of irony that may sidestep the mobilising force of fear that should legitimise the Government’s agenda.
Los recursos polifónicos como elementos lingüístico-persuasivos en la publicidad de la novela negra
Authors: Escribano Hernández Asunción 
Published: May 2017

Resumen
En este artículo vamos a mostrar cómo la argumentación lingüística de carácter polifónico contribuye a reflejar la identidad y la imagen social de la novela negra, a través de los enunciados publicitarios editoriales que la promocionan, constituyéndose, así, como un recurso lingüístico persuasivo de gran eficacia. Hemos estudiado los distintos modos de polifonía textual para comprobar cómo la polifonía es en este ámbito un mecanismo argumentativo muy poderoso con el que se consigue incentivar la compra, al tiempo que reflejar las características y peculiaridades de este género literario.
Age and Method of Release Affect Migratory Performance of Hatchery Steelhead
Authors: Tatara Christopher P. , Cooper Matt R. , Gale William , Kennedy Benjamen M. , Pasley Chris R. , Berejikian Barry A. 
Published: May 2017

Abstract
Hatchery programs that are designed to aid recovery of natural populations of anadromous salmonids, including steelhead Oncorhynchus mykiss, require locally derived, natural-origin broodstock. In such programs, achieving smoltification size thresholds may require extending hatchery rearing beyond age 1. We compared out-migration survival and travel rates of 142,990 PIT-tagged steelhead smolts released at age 1 (S1 rearing strategy) or age 2 (S2 rearing strategy) over five release years at Winthrop National Fish Hatchery (WNFH, Okanogan County, Washington). An S2 rearing cycle produced larger smolts with more uniform size distributions, resulting in higher survival during the first portion of their out-migration than for S1 smolts in three of the five release years. The S2 smolts migrated more rapidly to the ocean than S1 smolts in all years except 2011 and arrived in the Columbia River estuary 5.4 d earlier on average than the S1 smolts. The S1 steelhead that did not leave during the volitional release were subsequently forced from the hatchery to measure their survival. Nonvolitional S1 migrants were smaller and had survival rates that were 2.3–66.3 times lower than those of S1 steelhead that left WNFH on their own. The same was true for S2 steelhead, but the magnitude of the survival difference between volitional migrants and fish forced from the raceways was less variable and ranged from 2.5- to 4.6-fold. We conclude that S2 rearing can be a successful strategy for producing smolts from local natural-origin broodstock, with out-migration survival and travel times that are equivalent to or better than those of S1 smolts produced from nonlocal broodstock.
Received July 25, 2016; accepted March 31, 2017 Published online May 31, 2017
Internalized Smoking Stigma in Relation to Quit Intentions, Quit Attempts, and Current E-Cigarette Use
Authors: O'Connor Richard J. , Rees Vaughan W. , Rivard Cheryl , Hatsukami Dorothy K. , Cummings K. Michael 
Published: May 2017

Background Reducing the social acceptability of smoking is associated with lowered smoking prevalence. However, denormalization strategies can also contribute to the stigmatization that some smokers may feel about their smoking. Smoking stigma may be more acute if smokers are also members of other stigmatized groups, such as racial/ethnic minorities. This study examined correlates of smoking self- and felt-stigma and discrimination, among current smokers. Methods Participants were recruited in the United States via a national commercial consumer panel to complete a cross-sectional, Web-based survey. Participants were 1528 current cigarette smokers aged 14 and older. Measures included the Internalized Stigma of Smoking Inventory (ISSI), Heaviness of Smoking Index, quit intentions, past-year quit attempts, and current use of electronic cigarettes (e-cigarettes). Results Self-stigma was significantly associated with higher intent to quit in the next 6 months (odds ratio [OR] = 2.47, P < .01) and in the next 30 days (OR = 4.21, P < .01), relative to no intention to quit, as well as having made 1 or 2 quit attempts in the past year (OR = 1.60, P < .01) or 3 or more quit attempts (OR = 1.74, P < .01) and associated with daily e-cigarette use (OR = 1.73, P < .05). Felt-stigma was positively associated with intent to quit in the next 30 days (OR = 1.54, P < .01), having made 3 or more quit attempts in the past year (OR = 1.35, P < .01), and both daily (OR = 2.05, P < .05) and some-day (OR = 1.30, P < .05) e-cigarette use. Discrimination was associated only with increased odds of daily e-cigarette use (OR = 1.83, P < .05). Conclusions Smokers who reported greater feelings of stigmatization about their smoking were more likely to report having made recent quit attempts, report a stronger intention quit smoking in the future, and report use of e-cigarettes, suggesting that feelings of self-and felt-stigmatization are related to greater motivation to stop smoking.
Public debts of Arab countries: selected indicators
Authors: El-Khoury Gabi 
Published: May 2017

This statistical file is concerned with the issue of public debts in Arab countries. It assumes that public debt is a key source to fund the budget deficit in most Arab countries, and the rising public debt, particularly external debt, is increasingly becoming a concern for several countries in the region due to the pressure debt servicing might impose on these countries, which basically suffer an uncomfortable primary balance, in addition to the impact of crises in the region.
Table 1 provides indicators on domestic public debts with ratios of debts to GDP, while Table 2 gives figures of external public debts with debt ratios to GDP. Table 3 provides estimates of total public debts with their ratios to GDP, while Tables 4 and 5 show figures of external public debt service, ratios of debt servicing to exports of goods and services and external public debt service ratios to Arab governments’ revenues respectively.
Annotating function to differentially expressed LincRNAs in myelodysplastic syndrome using a network-based method
Authors: Liu Keqin , Beck Dominik , Thoms Julie A I , Liu Liang , Zhao Weiling , Pimanda John E , Zhou Xiaobo 
Published: May 2017

Abstract

Motivation
Long non-coding RNAs (lncRNAs) have been implicated in the regulation of diverse biological functions. The number of newly identified lncRNAs has increased dramatically in recent years but their expression and function have not yet been described from most diseases. To elucidate lncRNA function in human disease, we have developed a novel network based method (NLCFA) integrating correlations between lncRNA, protein coding genes and noncoding miRNAs. We have also integrated target gene associations and protein-protein interactions and designed our model to provide information on the combined influence of mRNAs, lncRNAs and miRNAs on cellular signal transduction networks.

Results
We have generated lncRNA expression profiles from the CD34+ haematopoietic stem and progenitor cells (HSPCs) from patients with Myelodysplastic syndromes (MDS) and healthy donors. We report, for the first time, aberrantly expressed lncRNAs in MDS and further prioritize biologically relevant lncRNAs using the NLCFA. Taken together, our data suggests that aberrant levels of specific lncRNAs are intimately involved in network modules that control multiple cancer-associated signalling pathways and cellular processes. Importantly, our method can be applied to prioritize aberrantly expressed lncRNAs for functional validation in other diseases and biological contexts.

Availability and implementation
The method is implemented in R language and Matlab.

Supplementary information
Supplementary data are available at Bioinformatics online.
The spike-and-slab lasso Cox model for survival prediction and associated genes detection
Authors: Tang Zaixiang , Shen Yueping , Zhang Xinyan , Yi Nengjun 
Published: May 2017

Abstract

Motivation
Large-scale molecular profiling data have offered extraordinary opportunities to improve survival prediction of cancers and other diseases and to detect disease associated genes. However, there are considerable challenges in analyzing large-scale molecular data.

Results
We propose new Bayesian hierarchical Cox proportional hazards models, called the spike-and-slab lasso Cox, for predicting survival outcomes and detecting associated genes. We also develop an efficient algorithm to fit the proposed models by incorporating Expectation-Maximization steps into the extremely fast cyclic coordinate descent algorithm. The performance of the proposed method is assessed via extensive simulations and compared with the lasso Cox regression. We demonstrate the proposed procedure on two cancer datasets with censored survival outcomes and thousands of molecular features. Our analyses suggest that the proposed procedure can generate powerful prognostic models for predicting cancer survival and can detect associated genes.

Availability and implementation
The methods have been implemented in a freely available R package BhGLM (http://www.ssg.uab.edu/bhglm/).

Supplementary information
Supplementary data are available at Bioinformatics online.
The probabilistic logic of communication and change
Authors: Achimescu Andreea , Baltag Alexandru , Sack Joshua 
Published: May 2017

Abstract
This article introduces a Probabilistic Logic of Communication and Change , which captures in a unified framework subjective probability, arbitrary levels of mutual knowledge and a mechanism for multi-agent Bayesian updates that can model complex social-epistemic scenarios, such as informational cascades. We show soundness, completeness and decidability of our logic, and apply it to a concrete example of cascade.