bluuebunny/crossref_metadata_2025_split huggingface dataset:
The substantiation of the composition of an extemporaneous cream with antipsoriatic and anti-inflammatory effects
Authors: Polova Zh. М. , Butkevych Т. А. , Diachenko V. Yu. , Simonova O. A. , Oliinyk S. V. 
Published: February 2025

Aim. To substantiate an extemporaneous emulsion cream based on purified naphthalan oil for the pharmacotherapy of psoriasis.
Materials and methods. Such methods as system analysis and generalization, as well as organoleptic, physicochemical, structural-mechanical, biopharmaceutical and statistical methods were used in the work.
Results. The composition of an extemporaneous agent in the form of a cream for external use with the antipsoriatic and anti-inflammatory action were substantiated. It contains a purified naphthalan oil, the chamomile oil extract and a 10 % oil solution of vitamin A as active components of the substance of natural origin. Six model samples of the emulsion cream were processed; Tween-80, emulsifier No. 1 and Olivem 1000 were introduced in them as emulsifiers. Glycerol and propylene glycol in the concentration of 10 % were used as structure-forming substances of the hydrophilic phase. Colloidal and thermal stability, pH, structural viscosity and kinetics of the active component release were determined by the agar diffusion method in order to substantiate the excipients in the composition of the emulsion cream.
Conclusions. As a result of the organoleptic, physicochemical, structural-mechanical and biopharmaceutical studies conducted, the composition of an extemporaneous emulsion cream with antipsoriatic and anti-inflammatory effects based on white purified naphthalan oil has been experimentally substantiated.
New Differential Subordination and Superordination Results for a subclass of Meromorphic Univalent Functions Defined by a New Operator
Authors: Sabri Mustafa A. , Atshan Waggas Galib , El-Seidy Essam 
Published: February 2025

In this paper, we explore and introduce a novel study employing a new operator denoted as W_(ν,γ,δ,τ,n,r,d)^(η,s) within the field of geometric function theory, particularly in the context of sandwich theorems. We derive results pertaining to differential subordination and superordination for this new formula operator. Additionally, we establish specific sandwich theorems.
Identification of testis derived differentially expressed spermatogenic microRNAs in idiopathic hypospermatogenesis
Authors: Sharma Nisha , Halder Ashutosh , Kaushal Seema , Kumar Manoj , Jain Manish 
Published: February 2025

Objectives
Hypospermatogenesis (HS) is a common histopathological subtype of non-obstructive azoospermia (NOA), characterized by a decrease in the total number of germ cells within the seminiferous tubule. The diagnosis of HS is made by invasive procedures like testicular biopsy or fine needle aspiration (FNA). MicroRNAs (miRs) are biomolecules with emerging roles as diagnostic biomarkers for diseases. This study aimed to investigate the differential miR expression profile in idiopathic HS (iHS) to explore candidate spermatogenic miRs in human male infertility.

Material and Methods
In this observational study, patients reporting azoospermia due to spermatogenic failure were recruited prospectively. Genetic exclusion was performed using XY-fluorescent in-situ hybridization (XY-FISH) and Yq microdeletion. Testicular FNA samples were used for total ribonucleic ccid (RNA) isolation. Small RNA sequencing-based exploratory analysis was performed on 20 iHS patients and five normospermatogenesis (NS) patients. nCounter miRNA expression based validation was performed for four iHS and four NS patients.

Results
Analysis of miRs in testicular tissue showed differential expression patterns having 49 downregulated and 3 upregulated miRs between iHS and NS patients, with miR-379-5p, miR-449a, miR-181c, miR-34b-3p, and miR-122b-5p being notable candidate spermatogenic miRNAs. Pathways such as Phosphatidylinositol 3 Kinase-Protein Kinase B (PI3K-Akt) and mitogen-activated protein kinase (MAPK) signaling and molecular functions like apoptosis and cell differentiation were significantly enriched in iHS patients. This is the first study of its kind to investigate the differential expression of micro-ribonucleic acid (miRs) in a cohort of NOA patients exclusively of the HS subtype. Comparing the study results to previously published data revealed that dysregulated spermatogenic miRs are shared in HS and other NOA subtypes. The analysis of spermatogenic miRs according to each patient’s profile showed significant dysregulation in miR expression, linked to 40% of cases with idiopathic HS.

Conclusion
This study provides important insight into the potential of miRNA to be used as a biomarker for the diagnosis of iHS. Although the study is based on a relatively low sample size, it provides a proof of concept which can be validated in a larger cohort.
O O REGIME DE AJUSTAMENTO, ESPACIALIDADE E INTERTEXTUALIDADE EM TORTO ARADO
Authors: Abriata Vera Lucia Rodella , Ribeiro Santos Flavia Karla , Fernandes Camilla , Cosci Tania Regina Cosci 
Published: February 2025

Esta pesquisa, que tem por base o instrumental teórico da semiótica discursiva e da Sociossemiótica, analisa o romance Torto Arado, de Itamar Vieira Júnior, com o objetivo de apreender a relação intertextual entre a obra e a capa do romance em sua edição brasileira, ilustrada por Aline Bispo. Outro objetivo é observar como o regime de interação por ajustamento se manifesta na construção dos sentidos do romance. Interessa-nos ainda desvelar como se projeta a espacialidade na narrativa e a correlação entre categorias do plano da expressão e do plano de conteúdo dos textos, que comporta grades de leitura possíveis, ou seja, valores recobertos por arranjos figurativos dos mais abstratos aos mais icônicos.
Assessment of the effectiveness of West Nile virus screening by analysing suspected positive donations among blood donors, Germany, 2020 to 2023
Authors: Orru' Stefano , Reissinger Annette , Filomena Angela , Heitmann Anna , Funk Markus Benedikt , Schmidt-Chanasit Jonas , Kreß Julia , Scheiblauer Heinrich , Cadar Dániel , Fiedler Sarah Anna 
Published: February 2025

Background
The first autochthonous human West Nile virus (WNV)-positive cases in Germany were confirmed in 2019. Risk minimisation measures (RMM) were introduced in 2020; no WNV transfusion-transmitted infections have been reported to date.

Aim
To analyse German suspected WNV-positive blood donations during annual seasons 2020–23 to review donor testing requirements.

Methods
WNV look-back procedures were initiated as per German regulations and additional donor data were collected. Blood samples were analysed by metagenomic next-generation sequencing (mNGS), individual donor nucleic acid amplification technique (ID-NAT)-based testing and antibody (Ab) testing.

Results
Seventy-four cases were followed up after WNV-positive donor mini-pool screening. Forty-five (83%) of 54 samples tested with the cobas WNV assay and 14 (29%) of 49 samples tested with the RealStar WNV assay showed a reactive ID-NAT-based result; the viral load ranged between 70,251 IU/mL and values below quantification limits. Fifteen (23%) of 64 samples serologically tested were reactive with at least one of the three Ab tests performed; the previous WNV-negative donation was nearly always documented > 28 days before. Of 73 samples sequenced, mNGS detected WNV in 26 (36%) and other flaviviruses in 14 (19%) cases.

Conclusion
In some suspected cases where a WNV infection was not confirmed, mNGS demonstrated a cross-reaction with other flaviviruses. Ab testing could only detect WNV in late stages of infection. A NAT-based WNV donor screening with a detection limit of at least 120 IU/mL seems to be a sufficiently effective RMM at present. However, a continuous re-evaluation of test strategy is always required.
Symptoms of postCOVID oral health conditions and their influence on the risk of developing dental pathology
Authors: Bekjanova O. E. , Rubnikovich S. P. , Kayumova V. R. , Denisova Yu. L. , Egorova K. Y. 
Published: February 2025

Post-COVID syndrome is diagnosed if symptoms last longer than 3 weeks after the onset of the disease, and a chronic course of the disease is determined if symptoms persist for more than 12 weeks. The investigation considered patients’ complaints and the presence of infection in the oral cavity following COVID-19 and, based on this, evaluated the influence of distant symptoms on the risk of developing dental pathology. All subjects were interviewed using the most commonly used questionnaire OHIP-14, which determines the level of quality of life related to dental health. The most frequent complaint was a feeling of dryness in the oral cavity, which was reported by 30.04 % of the subjects (1.64 % in the control group). More than 24.90 % of patients who had COVID-19 noted loss of taste sensation (in the control group ‒ 1.64 %). Clinical oral examination of COVID-19 patients revealed such oral mucosal lesions as erythema, papules, vesicles, as well as periodontal and salivary gland disorders.
Analysis of the genetic structure of Acinetobacter baumannii isolates, isolated from patients of the surgery and resuscitation departments of medical institutions in different regions of the Republic of Belarus
Authors: Yanovich O. O. , Titov L. P. 
Published: February 2025

A. baumannii bacteria are gram-negative, catalase-positive, oxidase-negative, non-fermenting coccobacilli that cause a wide range of local and systemic purulent-inflammatory processes. Genetic variants of bacteria that are highly resistant to antibiotics and cause healthcare-associated infections have acquired the greatest clinical significance. Multidrugresistant bacteria are widespread in medical institutions in different countries and pose a significant public health problem.We studied 53 isolates of A. baumannii isolated from patients in intensive care wards and surgical departments. Molecular genetic methods – multilocus sequencing-typing and real-time PCR – established the spectrum of sequence types (ST) and clonal complexes (CC), as well as carriers of extended type beta-lactamase resistance genes, metallo-beta-lactamases and serine carbapenemase.The isolates studied by the MLST method belonged to 4 clonal complexes: CC109, CC92, CC944 and CC110. The genetic structure of the dominant CC109 is represented by the following sequence types – ST2550, ST231, ST441. CC92 united ST168, ST450 and ST195. The minor CCs included CC944 and CC110.The phylogenetic analysis has established that the ancestral ST of cluster I is ST229, evolving to form sequence types ST195, ST450, as well as ST2563 and ST1103. Cluster 2 is formed on the basis of ST2182 evolving with the formation of two branches ST2564 and ST441.The analysis of the relationships between STs and CCs depending on the region of the country showed that isolates related to ST2550 (CC109) are more common in the Gomel, Mogilev, Minsk and Grodno regions, and ST231 (CC109) isolates are more common in the Vitebsk, Grodno and Mogilev regions.
Healthcare – Where Law and Economics Fail Society – Needs the “Laboratory of Democracy”
Authors: Waldman Deane 
Published: February 2025

In the U. S., legal structures as well as economic systems fail to provide people in society with accessible and affordable life-saving medical care. Other government-run national healthcare systems called single payers experience similar medical inadequacies and dollar inefficiencies though not the legal issues of the U.S.
Systems theory identifies the government command economy with third-party payment structure as the root cause of healthcare over-spending, variable quality, slow service, and death-by-queue.
An effective solution for healthcare system failures derives from the root cause. To make healthcare affordable and accessibl: restore rule of law, remove third-party decision-making, and infuse free market forces. The U.S. could test this hypothesis if states became “laboratories of democracy.”
Age-related features of iron metabolism in hanoverian mares
Authors: Karpenko L. , Bakhta A. , Balykina A. , Golodyaeva M. , Velikodnaya E. 
Published: February 2025

Purpose: to study age-related changes in iron metabolism indices in Hanoverian mares.Materials and methods. The study was conducted in the summer at a horse farm in the Tosnensky District of the Leningrad Region. For the study, 20 clinically healthy mares were selected, which were divided into 4 age groups of 5 horses each: the first group — 2—4 years old, the second — 5—10 years old, the third — 11—15 years old, the fourth — 16 years old and older. Blood was collected into vacutainer tubes for hematology with K3EDTA. The following parameters were determined in each sample: hemoglobin concentration (hemoglobin cyanide method using acetone cyanohydrin), serum iron content (determined using the ABRIS+ kit), total iron-binding capacity (TIBC, determined using the ABRIS+ kit), latent iron-binding capacity (LIBC, calculated value), transferrin content, transferrin saturation coefficient (TIC, calculated value), and erythrocyte concentration was counted using a counting chamber with a Goryaev grid. The reliability calculation was carried out using the Mann-Whitney criterion.Results. When analyzing the obtained data, it was revealed that the average indicators, except for the transferrin saturation coefficient, are within the reference values. The transferrin saturation coefficient exceeds the permissible values by an average of 24,06 %. The highest values of this indicator are observed in animals aged 2—4 years (33,8 %). A reliable increase in the number of erythrocytes by 34,3 % was also established in the 2—4 years age group compared to the 5–10 years group (p<0,05). A significant increase in hemoglobin was noted in the group of horses aged 5-10 years by 16,4 % (p<0,05) compared to the group aged 2—4 years, which may be a consequence of the body reaching physiological maturity. The transferrin saturation coefficient was also significantly higher by 7,9 % in horses aged 2—4 years compared to the average for all ages (p<0,05). Horses in the 16-year-old and older age group have higher average serum iron, total serum iron-binding capacity, and latent serum iron-binding capacity than other groups of horses.
Dynamics of changes in the quantitative and functional parameters of leukocytes when mineral feed additives are introduced into the diet
Authors: Panova N. , Prigozhaya O. 
Published: February 2025

Purpose: a comprehensive assessment of the effect of chelated forms of trace elements on quantitative and qualitative characteristics of leukocytes in combination with growth processes. Materials and methods. The experimental part was carried out on male laboratory rats. 2 homogeneous groups of 5 individuals were formed: experimental and control. The feed additive “Helamax C” was introduced to the main diet of the experimental group. The experiment was conducted in 3 stages. At each stage, body weight measurements were carried out, as well as blood sampling with subsequent examination of leukocytes (counting the absolute and relative number of leukocytes, building leukograms, as well as analysis of leukocyte indices). Blood was taken from the caudal vein. Leukocytes were counted according to the generally accepted method using the Goryaev camera. Leukograms were calculated at each stage of the experiment, after which leukocyte indices were calculated.Results. At the end of the experiment, there was an increase in the total number of leukocytes from 5,95±0,12 G/l to 7,47± 0,32 G/l in the blood of rats against the background of the use of a mineral supplement. A decrease in the absolute number of rod-shaped neutrophils from 0,19±0,08 G/l to 0,11±0,04 G/l, with a simultaneous increase in the level of segmented neutrophils to 1,39±0,18 G/l. In the rats of the experimental group, the absolute number of lymphocytes in the blood increased from 4.94±0,07 G/l to 5,64±0,26 G/l. There was an increase in the neutrophil lymphocyte ratio index from 0,06±0,03 to 0,26±0,02 in individuals of the experimental group, as well as a decrease in the nuclear shift index from 0,32±0,09 to 0,08±0,03. The body weight of the experimental rats increased from 368,40±20,27 g to 534,40±13,74 g.Conclusion. The introduction of mineral supplements into the diet helps to improve the quantitative and qualitative indicators of leukocytes, stimulating and regulating their formation, differentiation, functioning and apoptosis. In addition, minerals have a positive effect on the immune system, increasing the body's natural resistance. Together with the activation and regulation of metabolic processes, this leads to stimulation of body growth and an increase in body weight.
Ovarian response to polyovulatory stimulation in Holstein cows with different polymorphic variants of the DIO1 gene
Authors: Yermilova A. , Smekalova A. , Aleinikova O. , Lebedeva I. 
Published: February 2025

The response of the ovaries of cattle to polyovulatory stimulation is characterized by significant variability and may depend to some extent on the animal genotype. In Holstein cows with a weak superovulatory ovarian response, a decreased thyroid activity was detected on the day of insemination and one week after insemination. However, there is no information on the relationship between polymorphism of genes controlling the thyroid system and the ovarian response to polyovulatory stimulation in cattle.Objective: To analyze the relationship between polymorphic variants of the deiodinase type 1 gene (DIO1) at position 13149 and the ovarian response to polyovulatory stimulation in Holstein cows.Materials and methods. The experiments involved 37 Holstein cows of the first and second lactation, in which 2 to 9 cycles of polyovulation stimulation were studied. On the first day of insemination, the number of follicles with a diameter greater than 5 mm was recorded in each ovary using an ultrasound scanner. Seven days after insemination, the embryos were washed out and assessed, and the number of corpora lutea in both ovaries was determined. Analysis of single nucleotide polymorphism of the DIO1 gene at position 13149 was performed on DNA samples using real-time PCR.Results. The frequency of occurrence of cows with the GG, CG and CC genotypes was 48,65 %, 43,24 % and 8,11 %, respectively. A tendency towards an increase in the occurrence frequency of individuals with a large number of corpora lutea (> 6,0) on the 7th day after insemination was found in animals with the GG genotype compared to the CG genotype (55,6 % versus 25,0 %, p<0,1). At the same time, among cows homozygous for the G allele, individuals with a higher number of degenerated embryos (> 1.0) per 1 washout were more common (33,3 % versus 0 % in cows with the CG genotype, p<0.05). Concurrently, the occurrence frequency of cows with a large number of high-quality morulae and blastocysts washed out during one session (> 4,0) was slightly higher in the group with the GG genotype than with the CG genotype (33,3 % versus 12,5 %).Conclusions. The data obtained suggest that the response of the ovaries of Holstein cows to polyovulatory stimulation may partially depend on the polymorphism of the DIO1 gene at position 13149. Furthermore, they indicate that the effect of polymorphic gene variants on fertility traits in cattle should be considered in combination with the breed of animals.
Impact of Business Development Services on Performance of Micro-enterprises: Evidence from Field Survey in Kavreplanchok, Nepal
Authors: Neupane Rabindra Kumar , Basnet Bhupindra Jung 
Published: February 2025

This article reveals the relationship between Business Development Services (BDS) and micro-enterprise performance based on the survey undertaken in Kavrepalanchok District of Nepal. The survey was undertaken for the completion of Ph. D. dissertation on Business Development Services (BDS) for Enterprise Promotion in Nepal. The objective of this article is to analyze the relationship between the different components of BDS with enterprise performance development. The primary nature of data is used in this article collected from the field survey of micro-entrepreneurs at Kavrepalanchok District. A quantitative approach is used in this research. The population of this study is all the micro-entrepreneurs created in the Kavrepalanchok District by Business Development Service program of the Ministry of Industry, Nepal. The collected survey data has been analyzed using both descriptive and inferential statistical analysis techniques to examine the relationship between dependent and independent variables. Survey finding concluded that BDS components have a significant impact for improving performance of micro-enterprises having more than 90 per cent variability explained by BDS in the enterprise performance. This article contributes to lessen the dearth of publications in this topic in Nepal. Findings of this research will be useful to policy makers working in industry and enterprise development, management students, would-be entrepreneurs, and training and enterprise development service providers of Nepal.
Influence of Sodium Reduction Using KCL and MSG on the Nutritional Profile and Consumer Acceptability of Bhutanese Cheese Curries
Authors: Wangchuk Tenzin , On-nom Nattira , Chamchan Rungrat , Khemthong Chanakan , Thangsiri Sirinapa , Chupeerach Chaowanee 
Published: February 2025

Objective: The rise in non-communicable diseases (NCDs); such as cardiovascular diseases, hypertension, diabetes, and cancers, in Bhutan is linked to unhealthy diets that are high in salt and saturated fats. This study aimed to reformulate popular high-sodium Bhutanese cheese curries—Shakam Datshi and Kewa Datshi—by reducing their sodium content using potassium chloride (KCl) and monosodium glutamate (MSG), while preserving traditional flavors and improving their nutritional profiles.Material and Methods: Shakam Datshi and Kewa Datshi were identified as the highest sodium content dishes among commonly consumed Bhutanese meals. Sodium was partially replaced with KCl and MSG, and additional ingredient adjustments to align with WHO dietary guidelines. Sensory and Satiety evaluations with 50 Bhutanese panelists compared the modified recipes to the original and assessed the acceptance and fullness provided by the reformulated dishes in four healthy set menus.Results: The reformulation achieved a 20% reduction in sodium for Shakam Datshi and over 40% for Kewa Datshi. Potassium levels significantly increased to 377.47 mg in Shakam Datshi and 651.51 mg in Kewa Datshi. Both dishes also showed reductions in total and saturated fats. Sensory evaluations indicated that the modified recipes retained high overall liking scores, similar to the originals (7.96 vs. 8.28 for Shakam Datshi and 7.48 vs. 7.90 for Kewa Datshi). Satiety assessments revealed sustained feelings of fullness.Conclusion: This study demonstrated that significant sodium reduction in traditional Bhutanese cheese curries can be achieved without compromising taste or cultural authenticity. These findings provide a viable strategy to reduce hypertension in the Bhutan population through healthier dietary options.