bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spent culture medium analysis from individually cultured bovine embryos demonstrates metabolomic differences
Authors: Perkel Kayla J. , Madan Pavneesh 
Published: October 2017

SummarySpent culture medium can provide valuable information regarding the physiological state of a bovine preimplantation embryos through non-invasive analysis of the sum/depleted metabolite constituents. Metabolomics has become of great interest as an adjunct technique to morphological and cleavage-rate assessment, but more importantly, in improving our understanding of metabolism. In this study,in vitroproduced bovine embryos developing at different rates were evaluated using proton nuclear magnetic resonance (1H NMR). Spent culture medium from individually cultured embryos (2-cell to blastocyst stage) were divided into two groups based on their cleavage rate fast growing (FG) and slow growing (SG; developmentally delayed by 12–24 h), then analyzed by a 600 MHz NMR spectrometer. Sixteen metabolites were detected and investigated for sum/depletion throughout development. Data indicate distinct differences between the 4-cell SG and FG embryos for pyruvate (P< 0.05,n= 9) and at the 16-cell stage for acetate, tryptophan, leucine/isoleucine, valine and histidine. Overall sum/depletion levels of metabolites demonstrated that embryos produced glutamate, but consumed histidine, tyrosine, glycine, methionine, tryptophan, phenylalanine, lysine, arginine, acetate, threonine, alanine, pyruvate, valine, isoleucine/leucine, and lactate with an overall trend of higher consumption of these metabolites by FG groups. Principal component analysis revealed distinct clustering of the plain medium, SG, and FG group, signifying the uniqueness of the metabolomic signatures of each of these groups. This study is the first of its kind to characterize the metabolomic profiles of SG and FG bovine embryos producedin vitrousing1H NMR. Elucidating differences between embryos of varying developmental rates could contribute to a better understanding of embryonic health and physiology.
The potential roles of c-Jun N-terminal kinase (JNK) during the maturation and aging of oocytes produced by a marine protostome worm
Authors: Stricker Stephen A. , Ravichandran Niharika 
Published: October 2017

SummaryPrevious investigations have indicated that c-Jun N-terminal kinase (JNK) regulates the maturation and aging of oocytes produced by deuterostome animals. In order to assess the roles of this kinase in a protostome, oocytes of the marine nemertean worm Cerebratulus were stimulated to mature and subsequently aged before being probed with phospho-specific antibodies against active forms of JNK and maturation-promoting factor (MPF). Based on blots of maturing oocytes, a 40-kD putative JNK is normally activated during germinal vesicle breakdown (GVBD), which begins at 30 min post-stimulation with seawater, whereas treating immature oocytes with JNK inhibitors downregulates both the 40-kD JNK signal and GVBD, collectively suggesting a 40-kD JNK may facilitate oocyte maturation. Along with this JNK activity, mature oocytes also exhibit high levels of MPF at 2 h post-stimulation. However, by ~6–8 h post-GVBD, mature oocytes lose the 40-kD JNK signal, and at ~20–30 h of aging, an ~48-kD phospho-JNK band arises as oocytes deactivate MPF and begin to lyse during a necroptotic-like mode of death. Accordingly, JNK inhibitors reduce the aging-related 48-kD JNK phosphorylation while maintaining MPF activity and retarding oocyte degradation. Such findings suggest that a 48-kD JNK may help deactivate MPF and trigger death. Possible mechanisms by which JNK activation either together with, or independently of, protein neosynthesis might stimulate oocyte degradation are discussed.
Symptom Dimensions of Depression and Apathy and Their Relationship With Cognition in Parkinson’s Disease
Authors: Szymkowicz Sarah M. , Dotson Vonetta M. , Jones Jacob D. , Okun Michael S. , Bowers Dawn 
Published: October 2017

AbstractObjectives:Both depression and apathy, alone and in combination, have been shown to negatively affect cognition in patients with Parkinson’s disease (PD). However, the influence of specific symptom dimensions of depression and apathy on cognition is not well understood. The current study investigated the relationship between symptom dimensions of depression and apathy, based on factors identified in Kirsch-Darrow et al. (2011), and memory and executive function in PD.Methods:A sample of 138 non-demented individuals with PD (mean age=64.51±7.43 years) underwent neuropsychological testing and completed the Beck Depression Inventory, 2nd Edition, and Apathy Scale. Separate hierarchical regression models examined the relationship between symptom dimensions of depression and apathy (“pure” depressive symptoms, “pure” apathy, loss of interest/pleasure [anhedonia], and somatic symptoms) and three cognitive domain composites: immediate verbal memory, delayed verbal memory, and executive function.Results:After adjusting for general cognitive status and the influence of the other symptom dimensions, “pure” depressive symptoms were negatively associated with the delayed verbal memory composite (p<.034) and somatic symptoms were positively associated with the executive function composite (p<.026). No symptom dimensions were significantly related to the immediate verbal memory composite.Conclusions:Findings suggest that specific mood symptoms are associated with delayed verbal memory and executive function performance in non-demented patients with PD. Further research is needed to better understand possible mechanisms through which specific symptom dimensions of depression and apathy are associated with cognition in PD. (JINS, 2018,24, 269–282)
Population dynamics of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) and its parasitoids in Maharashtra State of India
Authors: Fand Babasaheb B. , Gaikwad Mahesh B. , Sul Nitin T. , Kumar Mahesh , Bhagat Kiran P. , Bal Santanu K. , Minhas P.S. 
Published: October 2017

Abstract
The cryptic nature and lack of consistent biological control of the stem fly, Melanagromyza sojae Zehntner, an emerging pest of soybean, Glycine max (L.) Merrill, necessitated the monitoring and assessment of the impact of the parasitoid complex on seasonal regulation of the pest population. In this paper, we measure and relate, using the host density as a predictor variable, the seasonal density dependence of M. sojae parasitoids and the level of parasitism in soybean fields. The results revealed density dependence of parasitioids. The populations built up concomitant with the host insect, reached their peak during the mid-season, and declined towards the end of the crop season. A complex of 10 species of hymenopteran parasitoids comprising six species from Pteromalidae and one species each from the families Eurytomitidae, Eucoilidae, Braconidae and Eulopidae had a significant impact on M. sojae populations with a peak parasitism of ~50%. The seasonality and relative abundance of parasitoids were explored. Eurytoma sp. (Eurytomitidae) and Gronotoma sp. (Eucoilidae) were the most prevalent parasitoids throughout the season, whereas the pteromalid Sphegigaster sp. was dominant during the mid-season. The modulation of management practices aimed at conservation of these bioagents could improve the biological control of M. sojae populations. We discuss the practicality of several management options aimed at achieving this goal.
Spin crossover-induced colossal positive and negative thermal expansion in a nanoporous coordination framework material
Authors: Mullaney Benjamin R. , Goux-Capes Laurence , Price David J. , Chastanet Guillaume , Létard Jean-François , Kepert Cameron J. 
Published: October 2017

AbstractExternal control over the mechanical function of materials is paramount in the development of nanoscale machines. Yet, exploiting changes in atomic behaviour to produce controlled scalable motion is a formidable challenge. Here, we present an ultra-flexible coordination framework material in which a cooperative electronic transition induces an extreme abrupt change in the crystal lattice conformation. This arises due to a change in the preferred coordination character of Fe(II) sites at different spin states, generating scissor-type flexing of the crystal lattice. Diluting the framework with transition-inactive Ni(II) sites disrupts long-range communication of spin state through the lattice, producing a more gradual transition and continuous lattice movement, thus generating colossal positive and negative linear thermal expansion behaviour, with coefficients of thermal expansion an order of magnitude greater than previously reported. This study has wider implications in the development of advanced responsive structures, demonstrating electronic control over mechanical motion.
Induction of synthetic lethality in IDH1-mutated gliomas through inhibition of Bcl-xL
Authors: Karpel-Massler Georg , Ishida Chiaki Tsuge , Bianchetti Elena , Zhang Yiru , Shu Chang , Tsujiuchi Takashi , Banu Matei A. , Garcia Franklin , Roth Kevin A. , Bruce Jeffrey N. , Canoll Peter , Siegelin Markus D. 
Published: October 2017

AbstractCertain gliomas often harbor a mutation in the activity center of IDH1 (R132H), which leads to the production of the oncometabolite 2-R-2-hydroxyglutarate (2-HG). In six model systems, including patient-derived stem cell-like glioblastoma cultures, inhibition of Bcl-xL induces significantly more apoptosis in IDH1-mutated cells than in wild-type IDH1 cells. Anaplastic astrocytoma samples with mutated IDH1 display lower levels of Mcl-1 than IDH1 wild-type tumors and specific knockdown of Mcl-1 broadly sensitizes glioblastoma cells to Bcl-xL inhibition-mediated apoptosis. Addition of 2-HG to glioblastoma cultures recapitulates the effects of the IDH mutation on intrinsic apoptosis, shuts down oxidative phosphorylation and reduces ATP levels in glioblastoma cells. 2-HG-mediated energy depletion activates AMPK (Threonine 172), blunting protein synthesis and mTOR signaling, culminating in a decline of Mcl-1. In an orthotopic glioblastoma xenograft model expressing mutated IDH1, Bcl-xL inhibition leads to long-term survival. These results demonstrate that IDH1-mutated gliomas are particularly vulnerable to Bcl-xL inhibition.
Cancer-specific PERK signaling drives invasion and metastasis through CREB3L1
Authors: Feng Yu-Xiong , Jin Dexter X. , Sokol Ethan S. , Reinhardt Ferenc , Miller Daniel H. , Gupta Piyush B. 
Published: October 2017

AbstractPERK signaling is required for cancer invasion and there is interest in targeting this pathway for therapy. Unfortunately, chemical inhibitors of PERK’s kinase activity cause on-target side effects that have precluded their further development. One strategy for resolving this difficulty would be to target downstream components of the pathway that specifically mediate PERK’s pro-invasive and metastatic functions. Here we identify the transcription factor CREB3L1 as an essential mediator of PERK’s pro-metastatic functions in breast cancer. CREB3L1 acts downstream of PERK, specifically in the mesenchymal subtype of triple-negative tumors, and its inhibition by genetic or pharmacological methods suppresses cancer cell invasion and metastasis. In patients with this tumor subtype, CREB3L1 expression is predictive of distant metastasis. These findings establish CREB3L1 as a key downstream mediator of PERK-driven metastasis and a druggable target for breast cancer therapy.
FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2
Authors: Porro Antonio , Berti Matteo , Pizzolato Julia , Bologna Serena , Kaden Svenja , Saxer Anja , Ma Yue , Nagasawa Kazuo , Sartori Alessandro A. , Jiricny Josef 
Published: October 2017

AbstractInterstrand cross-link (ICL) hypersensitivity is a characteristic trait of Fanconi anemia (FA). Although FANCD2-associated nuclease 1 (FAN1) contributes to ICL repair, FAN1 mutations predispose to karyomegalic interstitial nephritis (KIN) and cancer rather than to FA. Thus, the biological role of FAN1 remains unclear. Because fork stalling in FAN1-deficient cells causes chromosomal instability, we reasoned that the key function of FAN1 might lie in the processing of halted replication forks. Here, we show that FAN1 contains a previously-uncharacterized PCNA interacting peptide (PIP) motif that, together with its ubiquitin-binding zinc finger (UBZ) domain, helps recruit FAN1 to ubiquitylated PCNA accumulated at stalled forks. This prevents replication fork collapse and controls their progression. Furthermore, we show that FAN1 preserves replication fork integrity by a mechanism that is distinct from BRCA2-dependent homologous recombination. Thus, targeting FAN1 activities and its interaction with ubiquitylated PCNA may offer therapeutic opportunities for treatment of BRCA-deficient tumors.
Engineering the internal surfaces of three-dimensional nanoporous catalysts by surfactant-modified dealloying
Authors: Wang Zhili , Liu Pan , Han Jiuhui , Cheng Chun , Ning Shoucong , Hirata Akihiko , Fujita Takeshi , Chen Mingwei 
Published: October 2017

AbstractTuning surface structures by bottom-up synthesis has been demonstrated as an effective strategy to improve the catalytic performances of nanoparticle catalysts. Nevertheless, the surface modification of three-dimensional nanoporous metals, fabricated by a top-down dealloying approach, has not been achieved despite great efforts devoted to improving the catalytic performance of three-dimensional nanoporous catalysts. Here we report a surfactant-modified dealloying method to tailor the surface structure of nanoporous gold for amplified electrocatalysis toward methanol oxidation and oxygen reduction reactions. With the assistance of surfactants, {111} or {100} faceted internal surfaces of nanoporous gold can be realized in a controllable manner by optimizing dealloying conditions. The surface modified nanoporous gold exhibits significantly enhanced electrocatalytic activities in comparison with conventional nanoporous gold. This study paves the way to develop high-performance three-dimensional nanoporous catalysts with a tunable surface structure by top-down dealloying for efficient chemical and electrochemical reactions.
Reducing sarcolipin expression mitigates Duchenne muscular dystrophy and associated cardiomyopathy in mice
Authors: Voit Antanina , Patel Vishwendra , Pachon Ronald , Shah Vikas , Bakhutma Mohammad , Kohlbrenner Erik , McArdle Joseph J. , Dell’Italia Louis J. , Mendell Jerry R. , Xie Lai-Hua , Hajjar Roger J. , Duan Dongsheng , Fraidenraich Diego , Babu Gopal J. 
Published: October 2017

AbstractSarcolipin (SLN) is an inhibitor of the sarco/endoplasmic reticulum (SR) Ca2+ATPase (SERCA) and is abnormally elevated in the muscle of Duchenne muscular dystrophy (DMD) patients and animal models. Here we show that reducing SLN levels ameliorates dystrophic pathology in the severe dystrophin/utrophin double mutant (mdx:utr−/−) mouse model of DMD. Germline inactivation of one allele of the SLN gene normalizes SLN expression, restores SERCA function, mitigates skeletal muscle and cardiac pathology, improves muscle regeneration, and extends the lifespan. To translate our findings into a therapeutic strategy, we knock down SLN expression in 1-month oldmdx:utr−/−mice via adeno-associated virus (AAV) 9-mediated RNA interference. The AAV treatment markedly reduces SLN expression, attenuates muscle pathology and improves diaphragm, skeletal muscle and cardiac function. Taken together, our findings suggest that SLN reduction is a promising therapeutic approach for DMD.
Large-scale water collection of bioinspired cavity-microfibers
Authors: Tian Ye , Zhu Pingan , Tang Xin , Zhou Chunmei , Wang Jianmei , Kong Tiantian , Xu Min , Wang Liqiu 
Published: October 2017

AbstractLarge-scale and high-efficient water collection of microfibers with long-term durability still remains challenging. Here we present well-controlled, bioinspired spindle-knot microfibers with cavity knots (named cavity-microfiber), precisely fabricated via a simple gas-in-water microfluidic method, to address this challenge. The cavity-microfiber is endowed with unique surface roughness, mechanical strength, and long-term durability due to the design of cavity as well as polymer composition, thus enabling an outstanding performance of water collection. The maximum water volume collected on a single knot is almost 495 times than that of the knot on the cavity-microfiber. Moreover, the spider-web-like networks assembled controllably by cavity-microfibers demonstrate excellent large-scale and high-efficient water collection. To maximize the water-collecting capacity, nodes/intersections should be designed on the topology of the network as many as possible. Our light-weighted yet tough, low-cost microfibers with high efficiency in directional water transportation offers promising opportunities for large-scale water collection in water-deficient areas.
Genetic rescue increases fitness and aids rapid recovery of an endangered marsupial population
Authors: Weeks Andrew R. , Heinze Dean , Perrin Louise , Stoklosa Jakub , Hoffmann Ary A. , van Rooyen Anthony , Kelly Tom , Mansergh Ian 
Published: October 2017

AbstractGenetic rescue has now been attempted in several threatened species, but the contribution of genetics per se to any increase in population health can be hard to identify. Rescue is expected to be particularly useful when individuals are introduced into small isolated populations with low levels of genetic variation. Here we consider such a situation by documenting genetic rescue in the mountain pygmy possum, Burramys parvus. Rapid population recovery occurred in the target population after the introduction of a small number of males from a large genetically diverged population. Initial hybrid fitness was more than two-fold higher than non-hybrids; hybrid animals had a larger body size, and female hybrids produced more pouch young and lived longer. Genetic rescue likely contributed to the largest population size ever being recorded at this site. These data point to genetic rescue as being a potentially useful option for the recovery of small threatened populations.
Deletion of 3p13-14 locus spanning FOXP1 to SHQ1 cooperates with PTEN loss in prostate oncogenesis
Authors: Hieronymus Haley , Iaquinta Phillip J. , Wongvipat John , Gopalan Anuradha , Murali Rajmohan , Mao Ninghui , Carver Brett S. , Sawyers Charles L. 
Published: October 2017

AbstractA multigenic locus at 3p13-14, spanning FOXP1 to SHQ1, is commonly deleted in prostate cancer and lost broadly in a range of cancers but has unknown significance to oncogenesis or prognosis. Here, we report that FOXP1-SHQ1 deletion cooperates with PTEN loss to accelerate prostate oncogenesis and that loss of component genes correlates with prostate, breast, and head and neck cancer recurrence. We demonstrate that Foxp1-Shq1 deletion accelerates prostate tumorigenesis in mice in combination with Pten loss, consistent with the association of FOXP1-SHQ1 and PTEN loss observed in human cancers. Tumors with combined Foxp1-Shq1 and Pten deletion show increased proliferation and anaplastic dedifferentiation, as well as mTORC1 hyperactivation with reduced Akt phosphorylation. Foxp1-Shq1 deletion restores expression of AR target genes repressed in tumors with Pten loss, circumventing PI3K-mediated repression of the androgen axis. Moreover, FOXP1-SHQ1 deletion has prognostic relevance, with cancer recurrence associated with combined loss of PTEN and FOXP1-SHQ1 genes.
Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates
Authors: Simmons T. J. , Frandsen K. E. H. , Ciano L. , Tryfona T. , Lenfant N. , Poulsen J. C. , Wilson L. F. L. , Tandrup T. , Tovborg M. , Schnorr K. , Johansen K. S. , Henrissat B. , Walton P. H. , Lo Leggio L , Dupree P. 
Published: October 2017

AbstractLytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinus similis (LsAA9A) and Collariella virescens (CvAA9A). LsAA9A and CvAA9A cleave a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan. LsAA9A additionally cleaves isolated xylan substrates. The structures of CvAA9A and of LsAA9A bound to cellulosic and non-cellulosic oligosaccharides provide insight into the molecular determinants of their specificity. Spectroscopic measurements reveal differences in copper co-ordination upon the binding of xylan and glucans. LsAA9A activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage. Overall, these data show that AA9 LPMOs can display different apparent substrate specificities dependent upon both productive protein–carbohydrate interactions across a binding surface and also electronic considerations at the copper active site.
Superelasticity and cryogenic linear shape memory effects of CaFe2As2
Authors: Sypek John T. , Yu Hang , Dusoe Keith J. , Drachuck Gil , Patel Hetal , Giroux Amanda M. , Goldman Alan I. , Kreyssig Andreas , Canfield Paul C. , Bud’ko Sergey L. , Weinberger Christopher R. , Lee Seok-Woo 
Published: October 2017

AbstractShape memory materials have the ability to recover their original shape after a significant amount of deformation when they are subjected to certain stimuli, for instance, heat or magnetic fields. However, their performance is often limited by the energetics and geometry of the martensitic-austenitic phase transformation. Here, we report a unique shape memory behavior in CaFe2As2, which exhibits superelasticity with over 13% recoverable strain, over 3 GPa yield strength, repeatable stress–strain response even at the micrometer scale, and cryogenic linear shape memory effects near 50 K. These properties are acheived through a reversible uni-axial phase transformation mechanism, the tetragonal/orthorhombic-to-collapsed-tetragonal phase transformation. Our results offer the possibility of developing cryogenic linear actuation technologies with a high precision and high actuation power per unit volume for deep space exploration, and more broadly, suggest a mechanistic path to a class of shape memory materials, ThCr2Si2-structured intermetallic compounds.
Open-Cavity Spun Fiber Raman Lasers with Dual Polarization Output
Authors: Nuño Javier , Rizzelli Giuseppe , Gallazzi Francesca , Prieto Francisco , Pulido Concepción , Corredera Pedro , Wabnitz Stefan , Ania-Castanon Juan D. 
Published: October 2017

AbstractRandom distributed feedback fiber Raman lasers, where the feedback mechanism is provided by Rayleigh backscattering, have attracted a good deal of attention since they were first introduced in 2010. Their simple and flexible design, combined with good lasing efficiency and beam quality properties, comparable to those of standard cavity lasers, have led to multiple applications, particularly in the fields of fiber sensing and optical communications. In spite of these advances, the polarization properties of random fiber Raman lasers, which can strongly affect their performance in both sensing and communications, have barely been explored so far. In this article we experimentally and theoretically study the polarization properties of different open-cavity laser designs, based on either standard transmission fibers or low polarization-mode-dispersion spun fibers. By using high-power, highly-polarized pumps, we demonstrate controllable polarization-pulling and simultaneous lasing at close wavelengths with different output polarization properties in random distributed feedback fiber Raman lasers. These results advance our understanding of the polarization dynamics in ultralong lasers, and pave the way to the design of novel fiber laser sources capable of polarization-sensitive sensing and distributed amplification.