bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Multimedia Interaktif Berbasis Lectora Inspire Pada Materi Trigonometri Kelas X Di SMK Negeri 1 Paguyaman
Authors: Karim Sri Rahayu , Abbas Nurhayati , Pauweni Khardiyawan A.Y 
Published: October 2024

Abstrak Penelitian ini bertujuan untuk menghasilkan multimedia interaktif pada materi trigonometri untuk kelas X SMK Semester Genap. Penelitian ini menggunakan metodologi penelitian Research and Development (R&D). Penelitian pengembangan ini mengikuti model pengembangan four-D Model Thiagarajan, Semmel dan Semmel terdiri dari empat tahap yaitu Define, Design, Develop dan Disseminate. Hasil validasi yang diperoleh dari 3 ahli media dan 3 ahli materi adalah 89.8% dan 83.93% dengan kriteria “Sangat Valid Digunakan”. Kelayakan media diperoleh melalui hasil uji keterbacaan media oleh 5 orang siswa kelas X SMK Negeri 1 Paguyaman 82.32%, dengan kriteria “Sangat Layak Digunakan”. Kepraktisan media diperoleh melalui angket respon siswa dan guru matematika SMK Negeri 1 Paguyaman terhadap multimedia interaktif berbasis lectora inspire. Hasil angket respon siswa kelas X AKL SMK Negeri 1 Paguyaman 90.87%, Hasil angket respon guru matematika SMK Negeri 1 Paguyaman 95.8%, dengan kriteria “Sangat Praktis Digunakan”. Kesimpulan bahwa multimedia interaktif berbasis lectora inspire valid, layak, dan praktis digunakan pada materi trigonometri kelas X.Kata Kunci: Multimedia Interaktif, Lectora Inspire, Trigonometri.This study aims to produce interactive multimedia on trigonometry material for grade X students in vocational high school in the even semester. This research uses Research and Development (R&D) model. This development research follows the Thiagarajan, Semmel, and Semmel four-D development model consisting of four stages, namely Define, Design, Develop and Disseminate. The validation results obtained from 3 media experts and 3 material experts were 89.8% and 83.93%, with the "Very Valid Used" criteria. The eligibility of the media was obtained through the results of the media readability test by 5 students of grade X at SMK Negeri 1 Paguyaman of 82.32% with "Very Suitable for Use" criteria. The practicality of the media was obtained through a questionnaire on the responses of students and mathematics teachers at vocational high school Negeri 1 Paguyaman to interactive multimedia based on lectora inspire. The results of the response questionnaire of grade X AKL students at SMK Negeri Paguyaman were 90.87%. The results of the questionnaire of mathematics teachers at SMK Negeri 1 Paguyaman were 95.8%, with the "Very Practical to Use" criteria. In conclusion, the interactive multimedia based on lectora inspire is valid, feasible, and practical for class X on trigonometry material.Keywords: Interactive Multimedia, Lectora Inspire, Trigonometry.
Kaempferol enhances intestinal repair and inhibits the hyperproliferation of aging intestinal stem cells in Drosophila
Authors: Zhao Liusha , Luo Ting , Zhang Hong , Fan Xinxin , Zhang Qiaoqiao , Chen Haiyang 
Published: October 2024

IntroductionIntestinal stem cells (ISCs) are crucial for tissue repair and homeostasis because of their ability to self-renew and differentiate. However, their functionality declines significantly with age, resulting in reduced tissue regeneration and a higher risk of age-related diseases. Addressing this decline in ISC performance during aging presents a substantial challenge. The specific impact of nutrients or dietary elements on ISC adaptive resizing is urgent to explore.MethodsDrosophila ISCs are an ideal model for studying development and aging because of their genetic richness, ease of manipulation, and similarity to mammalian tissues. As the primary mitotically active cells in the Drosophila gut, ISCs are flexible in response to dietary and stress signals. Manipulating signaling pathways or dietary restrictions has shown promise in regulating ISC functions and extending lifespan in flies, these approaches face broader applications for aging research.ResultsKaempferol is well-regarded for its antioxidant, anti-inflammatory, and potential anticancer effects. However, its impacts on ISCs and the associated mechanisms remain inadequately understood. Our findings indicate that Kaempferol accelerates gut recovery after damage and improves the organism’s stress tolerance. Moreover, Kaempferol suppresses the hyperproliferation of aging ISCs in Drosophila. Further investigation revealed that the regulatory effects of Kaempferol on ISCs are mediated through the reduction of endoplasmic reticulum (ER) stress in aging flies and the modulation of excessive reactive oxygen species (ROS) levels via ER-stress pathways. Furthermore, Kaempferol exerts regulatory effects on the insulin signaling pathway, thereby contributing to the attenuation of ISC senescence.DiscussionThis study reveals that Kaempferol promotes intestinal homeostasis and longevity in aging flies by targeting ER stress and insulin signaling pathways, though the exact molecular mechanisms require further exploration. Future research will aim to dissect the downstream signaling events involved in these pathways to better understand how Kaempferol exerts its protective effects at the molecular level.
P-wave terminal force in lead V1 is associated with recurrence after catheter ablation in patients with paroxysmal atrial fibrillation and normal left atrial size
Authors: Wang Zhao , Wang Binhao , Yang Yiheng , Yang Xiaolei , Che Ying , Xia Yunlong 
Published: October 2024

BackgroundA previous investigation reported that an abnormal P-wave terminal force in lead V1 (PTFV1) is a marker for electrical remodeling of the left atrium (LA). We aimed to assess the relationship of PTFV1 with LA tachyarrhythmia (LATA) recurrence after radiofrequency catheter ablation (RFCA) in patients with paroxysmal atrial fibrillation (PAF) and normal LA size.MethodsPatients with PAF and normal LA size (LA volume index &lt; 34 ml/m2) who underwent RFCA were consecutively included between January 2018 and December 2020 and divided into two groups based on the presence (recurrence group) or absence (nonrecurrence group) of LATA recurrence. PTFV1 was measured according to preprocedural electrocardiography. The association between PTFV1 and the recurrence of LATA was investigated.ResultsA total of 385 patients were included. After a median follow-up period of 745 (467, 977) days, 109 (28.3%) patients experienced LATA recurrence. PTFV1 was greater in the recurrence group. Multivariate Cox regression analysis demonstrated that the hazard ratio and 95% confidence interval for PTFV1 per 1,000 μV*ms increase and PTFV1 &gt; 4,000 μV*ms were 1.22 (1.13–1.32, p &lt; 0.001) and 2.32 (1.54–3.48, p &lt; 0.001), respectively.ConclusionPTFV1 is an independent predictor for LATA recurrence after RFCA in patients with PAF and normal LA size.
Prognostic value of the left ventricular ejection fraction reserve acquired by gated myocardial perfusion SPECT in patients with CAD and reduced stress LVEF
Authors: Zhang Shuang , Meng Jingjing , Zhou Yihan , Lv Lijun , Zhang Xiaoli 
Published: October 2024

PurposeLeft ventricular ejection fraction (LVEF) strongly predicts cardiac events. However, conflicting findings exist regarding the prognostic value of the LVEF reserve (ΔLVEF) when measured by gated single-photon emission computed tomography myocardial perfusion imaging (SPECT G-MPI). In particular, data related to the prognostic value of ΔLVEF when measured by SPECT in patients with reduced LVEF are scarce. In this study, we aimed to evaluate the prognostic value of ΔLVEF when acquired by SPECT G-MPI in patients with coronary artery disease (CAD) and a LVEFStress &lt; 60%.MethodsWe retrospectively recruited 260 consecutive patients diagnosed with CAD by coronary angiography (CAG) and a LVEFStress &lt; 60%, as determined by SPECT G-MPI. These patients were followed up for 33.4 ± 7.6 months. The patients were divided into two groups (ΔLVEF &gt; 0% and ΔLVEF ≤ 0%), and survival analyses were conducted. The primary endpoints were major adverse cardiac events (MACEs), a composite of all-cause death, nonfatal myocardial infarction, unplanned coronary revascularization, and hospitalization for unstable angina.ResultsWe observed 69 MACEs (26.5%). The cumulative incidence of MACEs in patients with ΔLVEF ≤ 0% was significantly higher than in patients with ΔLVEF &gt; 0% (P = 0.042). Multivariate Cox regression further revealed that a ΔLVEF ≤ 0% represented an independent predictor of MACEs (adjusted hazard ratio [HR]: 1.276; 95% confidence interval [CI]: (1.006, 1.618), P = 0.045). Adding a ΔLVEF ≤ 0% to traditional myocardial perfusion and function variables evaluated by MPI significantly improved the ability to predict MACEs (P = 0.044).ConclusionsDetermining ΔLVEF by SPECT G-MPI was associated with MACEs and improved risk stratification compared to prediction models based on traditional perfusion and functional parameters in CAD patients with left ventricular dysfunction, particularly those with no or mild myocardial ischemia.
Protective effects of arecanut seed phenols in retinoic acid induced osteoporosis and the potential mechanisms explored by network pharmacology
Authors: Tang Min-min , Sun Li-ping , Song Fei , Chen Hua 
Published: October 2024

BackgroundArecanut seed is an important traditional medicine in Southeast Asia. It has been presented in a clinical formula to treat osteoporosis (OP) in China. Arecanut seed is abundant in phenols. However, most of current studies mainly focused on estrogen-deficient osteoporosis (OP) model of arecanut seed phenols (ASP), there is still a lack of roundly research about molecular mechanism of ASP therapy on OP and its influence on in drug-induced bone loss.Materials and methodsTo explore potential molecular mechanisms and the effects of ASP on OP, network pharmacology, molecular docking methods and a retinoic acid-induced OP rat model were employed in this study. According to the network pharmacology method, OP related targets and ASP compound related targets were collected from databases to obtain hub targets and top active chemicals in ASP treating OP. The potential therapic pathways were also calculated. Binding capacities of top active chemicals to hub targets were analyzed by molecular dock assay. In the animal experiment, osteocalcin (OCN) levels and alkaline phosphatase (ALP) activity in serum of all the rats were determined. The views of bone section were stained to observe the bone micro-structure of ASP affects. Bone mineral density (BMD), cortical bone thickness (CBT), area ratio of bone cortex (CAR) and area ratio of bone trabecula (TAR) were obtained from micro computed tomography to evaluate the effectiveness of ASP on bone loss.ConclusionThree hub genes and three top active compounds were screened by network pharmacology analysis and they combined well with each other. ASP had positive effects on alleviating RA-induced bone loss by regulating the expression of the hub genes. Signals in IL-17 pathway were predicted and primarily verified being potential targets in ASP treating OP.
High-resolution ocean color reconstruction and analysis focusing on Kd490 via machine learning model integration of MODIS and Sentinel-2 (MSI)
Authors: Yang Yulin , Wang Ziyao , Chen Peng , Shen Xue , Kong Wei , Huang Genghua , Shu Rong 
Published: October 2024

Oceanic water quality monitoring is essential for environmental protection, resource management, and ecosystem vitality. Optical remote sensing from space plays a pivotal role in global surveillance of oceanic water quality. However, the spatial resolution of current ocean color data products falls short of scrutinizing intricate small-scale marine features. This study introduces a hybrid model that fuses MODIS (Moderate Resolution lmaging Spectroradiometer) ocean color products with Sentinel-2 ‘s remote sensing reflectance data to generate high-resolution ocean color imagery, specifically investigating the diffuse attenuation coefficient at a wavelength of 490 nm (Kd490). To address the intricacies of coastal environments, we propose two complementary strategies to improve the accuracy of inversion. The first strategy leverages MODIS ocean color products alongside a geographic segmentation model to perform distinct inversions for separate marine zones, enhancing spatial resolution and specificity in coastal regions. The second strategy bolsters model interpretability during training by integrating predictions from conventional physical models into a Random Forest-based Regression Ensemble (RFRE) model. This study focuses on the coastal regions surrounding the Beibu Gulf, near Hainan Island in China. Our findings exhibit a strong concordance with MODIS products, achieving a monthly average coefficient of determination (R²) of 0.90, peaking at 0.97, and sustaining a monthly average root-mean-square error (RMSE) of less than 0.02. These results substantiate the model’s efficacy. Moreover, the annual trend analysis and localized assessment of the reconstructed Kd490 offer nuanced insights that surpass MODIS data, establishing a robust foundation for high-resolution water quality monitoring in coastal zones.
Towards harmonized laboratory methodologies in veterinary clinical bacteriology: outcomes of a European survey
Authors: Koritnik Tom , Cvetkovikj Iskra , Zendri Flavia , Blum Shlomo Eduardo , Chaintoutis Serafeim Christos , Kopp Peter A. , Hare Cassia , Štritof Zrinka , Kittl Sonja , Gonçalves José , Zdovc Irena , Paulshus Erik , Laconi Andrea , Singleton David , Allerton Fergus , Broens Els M. , Damborg Peter , Timofte Dorina 
Published: October 2024

IntroductionVeterinary clinical microbiology laboratories play a key role in antimicrobial stewardship, surveillance of antimicrobial resistance and prevention of healthcare associated-infections. However, there is a shortage of international harmonized guidelines covering all steps of veterinary bacterial culture from sample receipt to reporting.MethodsIn order to gain insights, the European Network for Optimization of Veterinary Antimicrobial Treatment (ENOVAT) designed an online survey focused on the practices and interpretive criteria used for bacterial culture and identification (C&amp;ID), and antimicrobial susceptibility testing (AST) of animal bacterial pathogens.ResultsA total of 241 microbiology laboratories in 34 European countries completed the survey, representing a mixture of academic (37.6%), governmental (27.4%), and private (26.5%) laboratories. The C&amp;ID turnaround varied from 1 to 2 days (77.8%) to 3–5 days (20%), and 6– 8 days (1.6%), with similar timeframes for AST. Individual biochemical tests and analytical profile index (API) biochemical test kits or similar were the most frequent tools used for bacterial identification (77% and 56.2%, respectively), followed by PCR (46.6%) and MALDI-TOF MS (43.3%). For AST, Kirby-Bauer disk diffusion (DD) and minimum inhibitory concentration (MIC) determination were conducted by 43.8% and 32.6% of laboratories, respectively, with a combination of EUCAST and CLSI clinical breakpoints (CBPs) preferred for interpretation of the DD (41.2%) and MIC (47.6%) results. In the absence of specific CBPs, laboratories used human CBPs (53.3%) or veterinary CBPs representing another body site, organism or animal species (51.5%). Importantly, most laboratories (47.9%) only report the qualitative interpretation of the result (S, R, and I). As regards testing for AMR mechanisms, 48.5% and 46.7% of laboratories routinely screened isolates for methicillin resistance and ESBL production, respectively. Notably, selective reporting of AST results (i.e. excluding highest priority critically important antimicrobials from AST reports) was adopted by 39.5% of laboratories despite a similar proportion not taking any approach (37.6%) to guide clinicians towards narrower-spectrum or first-line antibiotics.DiscussionIn conclusion, we identified a broad variety of methodologies and interpretative criteria used for C&amp;ID and AST in European veterinary microbiological diagnostic laboratories. The observed gaps in veterinary microbiology practices emphasize a need to improve and harmonize professional training, innovation, bacterial culture methods and interpretation, AMR surveillance and reporting strategies.
Incorporating platelet-to-white blood cell ratio into survival prediction models for intracerebral hemorrhage: a nomogram approach
Authors: Xu Jiake , Wang Xing , Chen Wei , Tian Meng , You Chao 
Published: October 2024

BackgroundPredicting long-term survival in intensive care unit patients with intracerebral hemorrhage (ICH) is crucial. This study aimed to develop a platelet-to-white blood cell ratio (PWR) incorporated nomogram for long-term survival prediction.MethodsA retrospective analysis was conducted on 1,728 ICH patients in the MIMIC-IV 2.2 database. The independent prognostic value of PWR for 1-year mortality was assessed. A nomogram was developed using LASSO and Cox regression to predict 1-year survival, incorporating PWR and other factors. The performance of the nomogram was evaluated through calibration curves, area under the curve, Delong test, net reclassification index, integrated discrimination improvement, and decision curve analysis.ResultsThe nomogram, which included age, weight, Glasgow Coma Scale (GCS) score, mechanical ventilation, glucose, red blood cell (RBC) count, blood urea nitrogen (BUN), and PWR, showed good predictive performance for 1-year survival. The C-index was 0.736 (95% CI = 0.716–0.756) for the training set and 0.766 (95% CI = 0.735–0.797) for the testing set. Higher age and ventilation increased mortality risk, while higher weight, GCS score, RBC count, and PWR decreased risk. The nomogram outperformed conventional scores.ConclusionsA nomogram incorporating PWR as a prognostic factor accurately predicts long-term survival in ICH patients. However, validation in large-scale multicenter studies and further exploration of biomarkers are needed.
Supplementary motor area is deactivated during mental rotation tasks with biomechanical constraints in fMRI
Authors: Nomura Makoto , Koeda Michihiko , Ikeda Yumiko , Tateno Amane , Arakawa Ryosuke , Aoyagi Yoichiro 
Published: October 2024

IntroductionMental rotation (MR) tasks of body parts involve anatomically interconnected brain systems. The systems are implicated in sensorimotor information integration and activate cortical motor-related areas, corresponding to the execution of similar motor tasks. In this study, we aimed to investigate the effect of varying the angle in the hand MR task on cerebral activation of the motor-related areas.MethodsTwenty healthy right-handed participants were recruited. We investigated cerebral activation while each participant decided whether a hand-palm image, rotated by 0°, 90°, 180°, and 270°, was a right or left hand.Results and discussionA significant negative correlation between the angle and brain activity was observed in the right and left supplementary motor area (SMA) and right posterior anterior cingulate gyrus. The SMA was inactivated with 180°- or 270°-rotated images in the regions of interest analysis. 180°- and 270°-rotated palms would be biomechanically difficult to position; thus, SMA deactivation may be closely associated with biomechanical constraints. This study provided novel findings regarding the neurophysiological mechanisms of motor imagery and may be useful in developing treatment plans using MR tasks during patient rehabilitation.
Evaluation of inflammatory hyperreflective foci and plasma EPA as diagnostic and predictive markers for age-related macular degeneration
Authors: Wan Zhongqi , Wu Yan , Shen Tianyi , Hu Chengyu , Lin Ruoyi , Ren Chengda , Yu Donghui , Li Tingting , Zhu Meijiang , Cai Wenting , Yu Jing 
Published: October 2024

ObjectivesTo detect the plasma polyunsaturated fatty acids (PUFAs) concentrations in age-related macular degeneration (AMD) patients and healthy controls. Additionally, advanced studies were conducted to investigate the relationship between PUFAs concentrations and ophthalmological characteristics, including hyperreflective foci (HRF), visual acuity, and anti-vascular endothelial growth factor (anti-VEGF) response in patients with AMD.MethodsThis prospective, single-site study recruited a total of 315 participants, consisting of 105 individuals with dry AMD (early-stage AMD group), 105 individuals with neovascular AMD (late-stage AMD group), and 105 elderly individuals without any fundus diseases (healthy controls). The levels of omega-3 and omega-6 PUFAs in plasma were detected using gas chromatography. Retinal thickness, choroidal thickness, and macular volume were quantified using optical coherence tomography angiography (OCTA) scan with a 6 × 6 mm macular area, and the amounts of HRF were analyzed with OCTA scanning data.ResultsCompared to the control group, AMD patients exhibited significantly lower plasma concentrations of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and alpha linolenic acid. HRF were observed in various retinal layers of AMD patients, particularly those with late-stage AMD. The correlation coefficient matrix and multiple linear regression models demonstrated that HRF played a crucial role in best corrected visual acuity for both early (p &lt; 0.001) and late-stage AMD patients (p = 0.006), while EPA had an inverse effect on the logarithm of the minimum angle of resolution (logMAR) value in patients with early-stage AMD (p &lt; 0.001). As compared to patients with good responses to anti-VEGF therapy, those with poor responses had significantly lower baseline logMAR (p &lt; 0.001), central retina thickness (p = 0.002), macular volume (p = 0.027), HRF (p = 0.024), and plasma EPA (p &lt; 0.001). This study used a ROC curve analysis to identify the combination of HRF and EPA as a potential biomarker for predicting the response to anti-VEGF treatment in late-stage AMD patients, with an area under the curve (AUC) value of 0.775.ConclusionsReduced plasma EPA was detected in AMD cases and the lower EPA concentration was related to poorer visual acuity. Additionally, the quantity of HRF combined with concentration of plasma EPA may serve as the prognostic indicator for predicting the effect of anti-VEGF treatment in late-stage AMD patients.