bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhangapi Virus: Genome Sequencing of a Brazilian Ungrouped Rhabdovirus Isolated in the Amazon Region
Authors: Wanzeller Ana Lucia M. , Nunes Márcio R. T. , Tavares Fernando N. , Pinto Walber V. M. , Júnior Edivaldo C. , de Lima Clayton P. S. , de Oliveira Layanna F. , Júnior João Lídio S. G. V. , Cardoso Jedson F. , Vasconcelos Pedro F. C. 
Published: January 2016

ABSTRACT

We report here nearly complete genome sequence of Inhangapi virus (INHV) strain BEAR177325, which was isolated from a pool of sandflies (
Lutzomyia flaviscutellata
) in the Utinga neighborhood, Belém (01º28´S 48°27′W), State of Pará, Brazil, in 1969. The genome of this virus showed similarity with members belonging to the family
Rhabdoviridae
.
Draft Genome Sequence of Streptomyces vitaminophilus ATCC 31673, a Producer of Pyrrolomycin Antibiotics, Some of Which Contain a Nitro Group
Authors: Mahan Kristina M. , Klingeman Dawn M. , Hettich Robert L. , Parry Ronald J. , Graham David E. 
Published: January 2016

ABSTRACT

Streptomyces vitaminophilus
produces pyrrolomycins, which are halogenated polyketide antibiotics. Some of the pyrrolomycins contain a rare nitro group located on the pyrrole ring. The 6.5-Mbp genome encodes 5,941 predicted protein-coding sequences in 39 contigs with a 71.9% G+C content.
Draft Genome Sequence of
Janthinobacterium
sp. Ant5-2-1, Isolated from Proglacial Lake Podprudnoye in the Schirmacher Oasis of East Antarctica
Authors: Koo Hyunmin , Strope Bailey M. , Kim Eddy H. , Shabani Adel M. , Kumar Ranjit , Crowley Michael R. , Andersen Dale T. , Bej Asim K. 
Published: January 2016

ABSTRACT

Janthinobacterium
sp. Ant5-2-1, isolated from the Schirmacher Oasis of East Antarctica, produces a purple-violet pigment, manifests diverse energy metabolism abilities, and tolerates cold, ultraviolet radiation, and other environmental stressors. We report here the 6.19-Mb draft genome of strain Ant5-2-1, which will help understand its survival mechanisms in extreme Antarctic ecosystems.
Complete Genome Sequences of the Obligate Symbionts “
Candidatus
Sulcia muelleri” and “
Ca.
Nasuia deltocephalinicola” from the Pestiferous Leafhopper
Macrosteles quadripunctulatus
(Hemiptera: Cicadellidae)
Authors: Bennett Gordon M. , Abbà Simona , Kube Michael , Marzachì Cristina 
Published: January 2016

ABSTRACT

Two bacterial symbionts of the European pest leafhopper,
Macrosteles quadripunctulatus
(Hemiptera: Cicadellidae), were fully sequenced. “
Candidatus
Sulcia muelleri” and “
Ca
. Nasuia deltocephalinicola” represent two of the smallest known bacterial genomes at 190 kb and 112 kb, respectively. Genome sequences are nearly identical to strains reported from the closely related host species,
M. quadrilineatus
.
Injury prevention strategies, coach compliance and player adherence of 33 of the UEFA Elite Club Injury Study teams: a survey of teams’ head medical officers
Authors: McCall Alan , Dupont Gregory , Ekstrand Jan 
Published: January 2016

Purpose(1) To quantify current practice at the most elite level of professional club football in Europe with regard to injury prevention strategy; (2) to describe player adherence and coach compliance to the overall injury prevention programme.MethodsA structured online survey was administered to the Head medical officers of 34 elite European teams currently participating in the UEFA Elite Club Injury Study. The survey had 4 sections; (1) risk factors for injury, (2) assessment and monitoring of injury risk, (3) prevention strategies and (4) coach compliance and player adherence to the injury prevention process.Results33 (97%) Medical officers of the teams responded. The most important perceived injury risk factor was previous injury. Four of the top 6 risk factors—physical fitness, accumulated fatigue, reduced recovery time between matches and training load—were related to player workload. The top 3 preventative exercises were eccentric, balance/proprioception and core training. Regarding monitoring, the top 3 tools implemented were measurement of workload, subjective wellness and a general medical screen. The subjectively rated level of coach compliance in UEFA teams was perceived as ‘high’, while the player adherence varied from none at all to perfect.Summary and conclusionMedical officers place importance on workload-related variables as risk factors for injury in elite European football players. A lack of consistently high player adherence may limit the effects of contemporary injury prevention programmes in elite European footballers.
Initial experience in setting up a medical student first responder scheme in South Central England
Authors: Seligman William H , Ganatra Sameer , England David , Black John J M 
Published: January 2016

Prehospital emergency medicine (PHEM) is a recently recognised subspecialty of emergency medicine, and anaesthetics, intensive care and acute medicine, in the UK, and yet it receives little to no mention in many undergraduate medical curricula. However, there is growing interest in PHEM among medical students and junior doctors. Several programmes are in existence across the UK that serve to provide teaching and exposure of prehospital care to medical students and junior doctors. However, relatively few students are able to gain significant first-hand experience of treating patients in the prehospital phase. In this short report, we discuss our experience of launching the student first responder (SFR) scheme across three counties in the Thames Valley. Medical students are trained by the regional ambulance service and respond to life-threatening medical emergencies in an ambulance response vehicle. The scheme is likely to benefit the ambulance service by providing a wider pool of trained volunteer first responders able to attend to emergency calls, to benefit patients by providing a quick response at their time of need, and to benefit medical students by providing first-hand experience of medical emergencies in the community. In its first 15 months of operation, SFRs were dispatched to 343 incidents. This scheme can serve as a training model for other ambulance services and medical schools across the UK.
Delays to initial reduction attempt are associated with higher failure rates in anterior shoulder dislocation: a retrospective analysis of factors affecting reduction failure
Authors: Kanji Avinash , Atkinson Paul , Fraser Jacqueline , Lewis David , Benjamin Susan 
Published: January 2016

IntroductionLittle is understood about the relationship between delay to treatment and initial reduction success for anterior shoulder dislocation. Our study examines whether delays to initial treatment, from injury and hospital presentation, are associated with higher reduction failure rates for anterior shoulder dislocation.MethodsA retrospective database and chart review was performed for patients undergoing intravenous sedation for attempted reduction of anterior shoulder dislocation in the emergency department (ED). Stepwise regression analysis was performed to identify predictors of reduction failure. Key variables analysed were the duration of the wait in the ED, the interval between the time of injury and first intervention and the interval from time of injury to arrival at the ED. Possible confounding variables analysed included age, gender, dose of sedative agent, qualifications of the reducing physician and whether the dislocated shoulder was recurrent.ResultsThe duration of the intervals from injury to first reduction attempt and from arrival at the ED to first reduction attempt were both independent predictors of a higher reduction failure rate (OR=1.07, 95% CI 1.02 to 1.13; OR=1.19, 95% CI 1.05 to 1.34). Every interval of 10 min increased the odds of a failed reduction attempt by 7% and 19%, respectively. Overall, shoulder reduction was successful during the initial sedation event in 97 cases (92%) and unsuccessful in nine cases (8%).ConclusionsDelays to first reduction attempt either from the time of injury or within the ED are associated with a lower reduction success rate for anterior shoulder dislocations.
Less frequently mutated genes in colorectal cancer: evidences from next-generation sequencing of 653 routine cases
Authors: Malapelle Umberto , Pisapia Pasquale , Sgariglia Roberta , Vigliar Elena , Biglietto Maria , Carlomagno Chiara , Giuffrè Giuseppe , Bellevicine Claudio , Troncone Giancarlo 
Published: January 2016

AimsThe incidence ofRAS/RAF/PI3KAandTP53gene mutations in colorectal cancer (CRC) is well established. Less information, however, is available on other components of the CRC genomic landscape, which are potential CRC prognostic/predictive markers.MethodsFollowing a previous validation study, ion-semiconductor next-generation sequencing (NGS) was employed to process 653 routine CRC samples by a multiplex PCR targeting 91 hotspot regions in 22 CRC significant genes.ResultsA total of 796 somatic mutations in 499 (76.4%) tumours were detected. BesidesRAS/RAF/PI3KAandTP53,other 12 genes showed at least one mutation includingFBXW7(6%),PTEN(2.8%),SMAD4(2.1%),EGFR(1.2%),CTNNB1(1.1%),AKT1(0.9%),STK11(0.8%),ERBB2(0.6%),ERBB4(0.6%),ALK(0.2%),MAP2K1(0.2%) andNOTCH1(0.2%).ConclusionsIn a routine diagnostic setting, NGS had the potential to generate robust and comprehensive genetic information also including less frequently mutated genes potentially relevant for prognostic assessments or for actionable treatments.
Optical coherence tomography versus intravascular ultrasound to evaluate stent implantation in patients with calcific coronary artery disease
Authors: Gudmundsdottir Ingibjorg , Adamson Philip , Gray Calum , Spratt James C , Behan Miles W , Henriksen Peter , Newby David E , Mills Nicholas , Uren Neal G , Cruden Nicholas L 
Published: January 2016

AimsStent underexpansion and malapposition are associated with adverse outcomes following percutaneous coronary intervention, but detection and treatment can be challenging in the presence of extensive coronary artery calcification. Frequency domain optical coherence tomography (FD-OCT) is a novel intravascular imaging technique with greater spatial resolution than intravascular ultrasound (IVUS) but its role in the presence of extensive coronary calcification remains unclear. We sought to determine the utility of FD-OCT compared to IVUS imaging to guide percutaneous coronary intervention in patients with severe calcific coronary artery disease.Methods18 matched IVUS and FD-OCT examinations were evaluated following coronary stent implantation in 12 patients (10 male; mean age 70±7 years) undergoing rotational atherectomy for symptomatic calcific coronary artery disease.ResultsIn-stent luminal areas were smaller (minimum in-stent area 6.77±2.18 vs 7.19±2.62 mm2, p<0.05), while reference lumen dimensions were similar with FD-OCT compared with IVUS. Stent malapposition was detected in all patients by FD-OCT and in 10 patients by IVUS. The extent of stent malapposition detected was greater (20% vs 6%, p<0.001) with FD-OCT compared to IVUS. Postdilation increased the in-stent luminal area (minimum in-stent area: 8.15±1.90 vs 7.30±1.62 mm2, p<0.05) and reduced the extent of stent malapposition (19% vs 34%, p<0.005) when assessed by FD-OCT, but not IVUS.ConclusionsAcute stent malapposition occurs frequently in patients with calcific coronary disease undergoing rotational atherectomy and stent implantation. In the presence of extensive coronary artery calcification, FD-OCT affords enhanced stent visualisation and detection of malapposition, facilitating improved postdilation stent apposition and minimal luminal areas.Trial Registration numberNCT02065102.
Structural, electronic and optical properties of hexagonal TaN compound
Authors: Chen Zhongjun , Yan Jungan , Kuang Zhong , Chen Taihong , Li Dehua 
Published: January 2016

Structural and electronic properties of hexagonal Tantalum nitride (TaN) in CoSn and WC structures are studied using the first-principle calculations. Lattice constants and electronic band structures are in an excellent agreement with the available experimental and other theoretical values. TaN in both structures studied has a metallic nature and a strong hybridization of Ta 5d and N 2p are found from the spin density of states (DOS). Meanwhile, our LSDA+U calculations predicted a strong ferromagnetic state for CoSn-type structure and an obvious paramagnetic nature for WC-type structure. No phase transition are observed within cubic and hexagonal CoSn and WC structures under high pressures. Our results show WC-type TaN is the calculated ground-state structure among the three crystallographic structures studied under 120 GPa. Optical properties show that TaN in CoSn-type structure is a better dielectric material.
miR-155 Drives Metabolic Reprogramming of ER+ Breast Cancer Cells Following Long-Term Estrogen Deprivation and Predicts Clinical Response to Aromatase Inhibitors
Authors: Bacci Marina , Giannoni Elisa , Fearns Antony , Ribas Ricardo , Gao Qiong , Taddei Maria Letizia , Pintus Gianfranco , Dowsett Mitch , Isacke Clare M. , Martin Lesley-Ann , Chiarugi Paola , Morandi Andrea 
Published: January 2016

Abstract
Aromatase inhibitors (AI) have become the first-line endocrine treatment of choice for postmenopausal estrogen receptor–positive (ER+) breast cancer patients, but resistance remains a major challenge. Metabolic reprogramming is a hallmark of cancer and may contribute to drug resistance. Here, we investigated the link between altered breast cancer metabolism and AI resistance using AI-resistant and sensitive breast cancer cells, patient tumor samples, and AI-sensitive human xenografts. We found that long-term estrogen deprivation (LTED), a model of AI resistance, was associated with increased glycolysis dependency. Targeting the glycolysis-priming enzyme hexokinase-2 (HK2) in combination with the AI, letrozole, synergistically reduced cell viability in AI-sensitive models. Conversely, MCF7-LTED cells, which displayed a high degree of metabolic plasticity, switched to oxidative phosphorylation when glycolysis was impaired. This effect was ER dependent as breast cancer cells with undetectable levels of ER failed to exhibit metabolic plasticity. MCF7-LTED cells were also more motile than their parental counterparts and assumed amoeboid-like invasive abilities upon glycolysis inhibition with 2-deoxyglucose (2-DG). Mechanistic investigations further revealed an important role for miR-155 in metabolic reprogramming. Suppression of miR-155 resulted in sensitization of MCF7-LTED cells to metformin treatment and impairment of 2-DG–induced motility. Notably, high baseline miR-155 expression correlated with poor response to AI therapy in a cohort of ER+ breast cancers treated with neoadjuvant anastrozole. These findings suggest that miR-155 represents a biomarker potentially capable of identifying the subset of breast cancers most likely to adapt to and relapse on AI therapy. Cancer Res; 76(6); 1615–26. ©2016 AACR.
Vozes negras na cantoria (1870-1925) O caso de Severino Perigo
Authors: Iumatti Paulo Teixeira 
Published: January 2016

<p>Trata-se de investigar a formação de um espaço ritual de interseção entre negros e “brancos”, em meio à sociedade escravista, no âmbito do universo da cantoria em províncias do chamado Norte do Brasil a partir de meados do século XIX. Tal espaço se constituiu em meio à crise da escravidão como instituição, e se articulou, a partir da Abolição, de forma a não favorecer a presença dos cantadores negros – que, no entanto, continuaram, em parte, atuantes. Aspectos desse processo de exclusão, que envolveu, dentre outros fatores, disputas acirradas pela memória na sociedade, nas obras dos folcloristas e nos folhetos de cordel, são estudados pela análise dos rastros da trajetória e da performance do cantador “não profissional” paraibano Severino Perigo, registrado em 1925 por Leonardo Mota. Argumentamos que, na leitura crítica da relação folclorista/informante, o estudo das construções simbólicas em torno do corpo e da voz é particularmente profícuo.</p><p> </p>
Escritas de ouvido na literatura brasileira
Authors: Rocha Marília Librandi 
Published: January 2016

<p>Este texto propõe que no coração da literatura brasileira encontra-se o pulsar de uma escrita auditiva, elaborada por autores que “escrevem de ouvido”. O objetivo é conceituar o termo “escrita de ouvido”, e descrever a forma que assume na prosa de ficção, em especial no romance. Machado de Assis, Clarice Lispector e Guimarães Rosa são os escritores centrais dessa pesquisa, que também se aplica a outros escritores-chave como Oswald de Andrade, Mário de Andrade e Graciliano Ramos. Pensa-se pois o ouvido como um terceiro termo para além da dicotomia entre a fala e a escrita, o romance, como um espaço de escuta, e a autoria, como um lugar de recepção mais do que de producão. Busca-se assim contribuir para uma poética da ressonância e uma história aural da literatura escrita em português com acentos multilíngues.</p>
Dossiê Figuras do estranhamento
Authors: Passos Cleusa Rios , Riaudel Michel , Rosenbaum Yudith 
Published: January 2016

<div class="WordSection1"><h1><span style="font-size: 10px;">Apresentação</span></h1><p> </p><h2><em>Primeiras reflexões</em></h2><p><span style="font-size: 10px;">“Figuras do estranhamento” pretende ser um território de múltiplas faces, abrindo-se para estudos de saberes diversos – literários, sociais, políticos, psicanalíticos, entre outros –, articulados em torno de...</span></p></div>
Discoidin Domain Receptor-1 Regulates Calcific Extracellular Vesicle Release in Vascular Smooth Muscle Cell Fibrocalcific Response via Transforming Growth Factor-β Signaling
Authors: Krohn Jona B. , Hutcheson Joshua D. , Martínez-Martínez Eduardo , Irvin Whitney S. , Bouten Carlijn V.C. , Bertazzo Sergio , Bendeck Michelle P. , Aikawa Elena 
Published: January 2016

Objective—
Collagen accumulation and calcification are major determinants of atherosclerotic plaque stability. Extracellular vesicle (EV)–derived microcalcifications in the collagen-poor fibrous cap may promote plaque rupture. In this study, we hypothesize that the collagen receptor discoidin domain receptor-1 (DDR-1) regulates collagen deposition and release of calcifying EVs by vascular smooth muscle cells (SMCs) through the transforming growth factor-β (TGF-β) pathway.

Approach and Results—

SMCs from the carotid arteries of DDR-1
−/−
mice and wild-type littermates (n=5–10 per group) were cultured in normal or calcifying media. At days 14 and 21, SMCs were harvested and EVs isolated for analysis. Compared with wild-type, DDR-1
−/−
SMCs exhibited a 4-fold increase in EV release (
P
<0.001) with concomitantly elevated alkaline phosphatase activity (
P
<0.0001) as a hallmark of EV calcifying potential. The DDR-1
−/−
phenotype was characterized by increased mineralization (Alizarin Red S and Osteosense,
P
<0.001 and
P
=0.002, respectively) and amorphous collagen deposition (
P
<0.001). We further identified a novel link between DDR-1 and the TGF-β pathway previously implicated in both fibrotic and calcific responses. An increase in TGF-β1 release by DDR-1
−/−
SMCs in calcifying media (
P
<0.001) stimulated p38 phosphorylation (
P
=0.02) and suppressed activation of Smad3. Inhibition of either TGF-β receptor-I or phospho-p38 reversed the fibrocalcific DDR-1
−/−
phenotype, corroborating a causal relationship between DDR-1 and TGF-β in EV-mediated vascular calcification.

Conclusions—
DDR-1 interacts with the TGF-β pathway to restrict calcifying EV-mediated mineralization and fibrosis by SMCs. We therefore establish a novel mechanism of cell-matrix homeostasis in atherosclerotic plaque formation.
Programmed Ventricular Stimulation for Risk Stratification in the Brugada Syndrome
Authors: Sroubek Jakub , Probst Vincent , Mazzanti Andrea , Delise Pietro , Hevia Jesus Castro , Ohkubo Kimie , Zorzi Alessandro , Champagne Jean , Kostopoulou Anna , Yin Xiaoyan , Napolitano Carlo , Milan David J. , Wilde Arthur , Sacher Frederic , Borggrefe Martin , Ellinor Patrick T. , Theodorakis George , Nault Isabelle , Corrado Domenico , Watanabe Ichiro , Antzelevitch Charles , Allocca Giuseppe , Priori Silvia G. , Lubitz Steven A. 
Published: January 2016

Background—
The role of programmed ventricular stimulation in identifying patients with Brugada syndrome at the highest risk for sudden death is uncertain.

Methods and Results—

We performed a systematic review and pooled analysis of prospective, observational studies of patients with Brugada syndrome without a history of sudden cardiac arrest who underwent programmed ventricular stimulation. We estimated incidence rates and relative hazards of cardiac arrest or implantable cardioverter-defibrillator shock. We analyzed individual-level data from 8 studies comprising 1312 patients who experienced 65 cardiac events (median follow-up, 38.3 months). A total of 527 patients were induced into arrhythmias with up to triple extrastimuli. Induction was associated with cardiac events during follow-up (hazard ratio, 2.66; 95% confidence interval [CI], 1.44–4.92,
P
<0.001), with the greatest risk observed among those induced with single or double extrastimuli. Annual event rates varied substantially by syncope history, presence of spontaneous type 1 ECG pattern, and arrhythmia induction. The lowest risk occurred in individuals without syncope and with drug-induced type 1 patterns (0.23%, 95% CI, 0.05–0.68 for no induced arrhythmia with up to double extrastimuli; 0.45%, 95% CI, 0.01–2.49 for induced arrhythmia), and the highest risk occurred in individuals with syncope and spontaneous type 1 patterns (2.55%, 95% CI, 1.58–3.89 for no induced arrhythmia; 5.60%, 95% CI, 2.98–9.58 for induced arrhythmia).

Conclusions—
In patients with Brugada syndrome, arrhythmias induced with programmed ventricular stimulation are associated with future ventricular arrhythmia risk. Induction with fewer extrastimuli is associated with higher risk. However, clinical risk factors are important determinants of arrhythmia risk, and lack of induction does not necessarily portend low ventricular arrhythmia risk, particularly in patients with high-risk clinical features.
Sensitivity of Early Brain Computed Tomography to Exclude Aneurysmal Subarachnoid Hemorrhage
Authors: Dubosh Nicole M. , Bellolio M. Fernanda , Rabinstein Alejandro A. , Edlow Jonathan A. 
Published: January 2016

Background and Purpose—
Emerging evidence demonstrating the high sensitivity of early brain computed tomography (CT) brings into question the necessity of always performing lumbar puncture after a negative CT in the diagnosis of spontaneous subarachnoid hemorrhage (SAH). Our objective was to determine the sensitivity of brain CT using modern scanners (16-slice technology or greater) when performed within 6 hours of headache onset to exclude SAH in neurologically intact patients.

Methods—
After conducting a comprehensive literature search using Ovid MEDLINE, Ovid EMBASE, Web of Science, and Scopus, we conducted a meta-analysis. We included original research studies of adults presenting with a history concerning for spontaneous SAH and who had noncontrast brain CT scan using a modern generation multidetector CT scanner within 6 hours of symptom onset. Our study adheres to the preferred reporting items for systematic reviews and meta-analyses (PRISMA).

Results—
A total of 882 titles were reviewed and 5 articles met inclusion criteria, including an estimated 8907 patients. Thirteen had a missed SAH (incidence 1.46 per 1000) on brain CTs within 6 hours. Overall sensitivity of the CT was 0.987 (95% confidence intervals, 0.971–0.994) and specificity was 0.999 (95% confidence intervals, 0.993–1.0). The pooled likelihood ratio of a negative CT was 0.010 (95% confidence intervals, 0.003–0.034).

Conclusions—
In patients presenting with thunderclap headache and normal neurological examination, normal brain CT within 6 hours of headache is extremely sensitive in ruling out aneurysmal SAH.