bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid real‐time PCR assay for detecting Microdochium paspali causing sparse leaf patch on seashore paspalum and in environmental samples
Authors: Kong Yixuan , Jin Peiyuan , Jia Chenchen , Qiao Panpan , Zhang Huangwei , Dong Yinglu , Yang Zhimin , Zhou Yuxin , Jung Geunhwa , Hu Jian 
Published: September 2024

AbstractBACKGROUNDSparse leaf patch (SLP) is one of the most significant diseases affecting seashore paspalum (Paspalum vaginatum Sw.), caused by Microdochium paspali. Fast and accurate detection of this pathogen is crucial for effective disease management. However, conventional culture‐based methods are time‐consuming and often compromised by the presence of other saprophytic or endophytic fungi.RESULTSIn this study, we developed a real‐time fluorescent quantitative (q)PCR method based on the internal transcribed spacer (ITS) region of the ribosomal RNA gene to rapidly detect and quantify M. paspali. The qPCR assay demonstrated the ability to detect all 12 tested isolates of M. paspali, with no cross‐reactions observed when tested against 30 isolates of other fungal pathogens from turfgrass samples. The detection limit of the qPCR method was as low as 3.65 × 102 copies μL−1 of M. paspali genomic DNA, and the entire detection process could be completed within 1 h. The fluorescence signal was detectable in the leaf tissues of seashore paspalum without apparent disease symptoms as early as 24 h postinoculation with M. paspali. Moreover, the qPCR method successfully detected M. paspali in both asymptomatic and symptomatic turfgrass samples, including leaf, stem, root and rhizosphere soil, indicating that this assay can significantly enhance the detection of M. paspali.CONCLUSIONThe study developed a rapid real‐time qPCR assay for the detection of M. paspali causing SLP on seashore paspalum and in environmental samples, which has important implications for early warning and management of SLP. © 2024 Society of Chemical Industry.
This is the first in‐human trial and prospective case series of a novel single‐port robotic system for gynaecological surgery: An IDEAL stage 2a study
Authors: Hu Xueli , Ruan Mengjia , Zhu Luoyi , Huang Menghan , Qi Lifang , Huang Min , Zou Shuangwei , Lin Lili , Zheng Wenwen , Wang Lizhi , Duan Ping 
Published: September 2024

AbstractBackgroundThe Shurui® system (SR‐ENS‐600) is a novel fully integrated single‐port robotic system with bioinspired serpentine surgical manipulators and a camera.MethodsThis was a single‐centre prospective case‐series study according to the IDEAL stage 2a guidelines to evaluate the feasibility, safety and potential efficacy of the Shurui® system for gynaecological surgeries and to improve the operating process.ResultsTen patients with a gradient of surgical difficulty who had indications for laparoscopic surgery and who volunteered to participate in a clinical trial were enrolled in the study. All 10 subjects successfully completed the procedure without converting to other procedures. No serious complications were reported at the 3‐month follow‐up. Subjects recover faster after surgery and are highly satisfied with the incision.ConclusionsGynaecological single‐site laparoscopic surgery with the Shurui® system was technically feasible for well‐selected patients with minimal alterations in technique. Further prospective multicenter large‐sample studies are necessary.Registration numberChiCTR2300075431. URL: https://www.chictr.org.cn/showproj.html?proj=189995.
Soy protein processing impacts the ice recrystallization inhibition activity of protein hydrolysates
Authors: Fomich Madison , Dia Vermont , Wang Tong 
Published: September 2024

AbstractSoy protein hydrolysates have demonstrated moderate ice recrystallization inhibition (IRI) activity, but the properties of the unhydrolyzed protein contributing to this activity are not well known. The objective of this research was to identify the main protein processing factors important to the varying IRI activities observed. Three possible modification reactions were applied to soy protein isolate (SPI): the Maillard reaction that can occur in soy flour before protein isolation, heat denaturation of the fully defatted protein, and heat denaturation of the protein with the presence of residual lipid, mainly polar phospholipids. These modified proteins were hydrolyzed by Alcalase protease (for 2 min) to produce hydrolysates, which were characterized by HPLC and FTIR to investigate how molecular weight (MW) and changes in secondary structure relate to IRI activity. A multilinear regression with the parameters of modification type, surface hydrophobicity, secondary structure profile, and average MW of the hydrolysates was used to investigate their roles in reducing ice crystal size. It was discovered that polar lipids present in the soy hydrolysate samples and MW were the only significant factors (p < 0.05) for IRI activity of SPI hydrolysates which had an ice crystal size reduction from 22.0% to 36.7%. This study demonstrates for the first time a protein hydrolysate‐phospholipid interaction can produce IRI active molecules.
Photoinduced Magnetization Switching in Organic Molecules Achieved Through Stabilization of the Triplet Excited States
Authors: Sääsk Verner , Wu Shu‐Qi , Sato Osamu 
Published: September 2024

AbstractOrganic photoresponsive materials, which change their physical properties upon irradiation with light, are attracting tremendous scientific attention, as they offer a flexible and sustainable platform for developing various optoelectronic devices. In this work, photoinduced magnetization switching in two carbazole‐functionalized organic molecules, (9H‐carbazol‐9‐yl)(4‐chlorophenyl)methanone (ClPhCz) and (9H‐carbazol‐9‐yl)(2,4‐dichlorophenyl)methanone (DClPhCz) is reported for the first time. It was found that stabilization of the triplet excited state in those molecules is long enough for a net change in magnetic moment to be detected with a SQUID magnetometer. The change in magnetic moment upon excitation with a blue light laser was larger in ClPhCz, remaining high upon repeating the photoirradiation. In contrast, DClPhCz exhibited a smaller magnetic response, which gradually decreased upon repeating the experiment.
Extraordinary Corrosion Inhibition of Potassium Stannate and Riboflavin Hybrid Additive on the Al Alloy Anode of Alkaline Al‐Air Batteries
Authors: Huang Yongbiao , Guo Lei , Ritacco Ida , Zhang Renhui , Sun Rui , Leng Senlin , Chang Jun 
Published: September 2024

AbstractAl‐air batteries (AABs) are regarded as a promising future alternative energy source due to their supposedly high energy density. The corrosion of AABs resulting from the hydrogen evolution corrosion and self‐corrosion occurring during their use in alkaline solutions represented a significant obstacle to their development, considerably limiting their commercialization. In this study, we examined the corrosion inhibition efficiency of the hybrid anticorrosive agent composed of potassium stannate (K2SO3) and riboflavin (Rib) when used as an addition in the electrolyte of an alkaline AAB. The experimental results demonstrated that the incorporation of the hybrid corrosion inhibitor leads to an anticorrosion inhibition efficiency of 70.2%, an increase in the discharge specific capacity, and specific energy of the entire battery from 913 mAh g−1 and 1023 Wh kg−1 to 2128 mAh g−1 and 2511 Wh kg−1, respectively. The adsorption behavior of the additives on the surface of the Al alloy was studied using surface characterization techniques such as scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy (EDS), atomic force microscopy (AFM), and X‐ray photoelectron spectroscopy (XPS). It revealed that the incorporation of the hybrid additive leads to the formation of a dense and uniform protective layer on the surface of the Al anode during the discharge process. This film effectively prevents direct contact between the alkaline electrolyte and the Al surface, drastically reducing the formation of possible hydrogen interactions between the electrolyte and the anode. Consequently, the hydrogen evolution reaction (HER) is efficiently inhibited, and the overall performance of the AAB is improved. Thus, this electrolyte regulation strategy presents a novel approach to studying high‐efficiency alkaline AABs.
Infrared Stealth Coating with Tunable Structural Color Based on ZnO Spheres
Authors: Ren Jie , Xie Chuwei , Zong Hao , Zhang Shufen , Wu Suli 
Published: September 2024

AbstractIt is important to develop low infrared (IR) emissive coating with tunable structure color to improve the infrared–visible stealth performance of military equipment. In this work, uniform ZnO spheres are used as building units to construct photonic structures with both bright adjustable structure color and low IR emissivity due to the relatively high refractive index and low IR emissivity of ZnO. The vivid tunable structural colors are provided by the photonic bandgap of ZnO photonic crystals (PCs) or the quasi‐bandgap of amorphous photonic crystals (APCs), respectively. Both ZnO PCs and APCs exhibited low IR emissivity in 3–5 µm. The IR emissivity of 255 nm ZnO PC is 0.483 and the IR emissivity of 255 nm ZnO APC is 0.492 at 25 °C. With the increase of temperature, the IR emissivity of further decreased to 0.295 and 0.312 at 300 °C. These structures can be applied to a variety of surfaces, and all these structures have good thermal and light stability as well. This work may open a simple and effective way to fabricate materials with good infrared–visible stealth performance, expanding the application of ZnO PCs and APCs coatings in the camouflage area.
NIR‐II Orthogonal Fluorescent Ratiometric Nanoprobe for In Situ Bioimaging of Carbon Monoxide
Authors: Guo Lichao , Wang Qingyuan , Gao Feng , Liang Ying , Ma Haoyue , Chen Desheng , Zhang Yue , Ju Huangxian , Zhang Xiaobo 
Published: September 2024

AbstractCarbon monoxide (CO) functions as a significant endogenous cell signaling molecule and is strongly associated with many physiological and pathological processes. However, conventional fluorescence imaging in the visible and near‐infrared (NIR) I regions suffers autofluorescence background and photon scattering, hindering the accurate detection of CO in vivo. In addition, the complexity of physiological environments leads to fluctuating fluorescence emission. To solve these problems, herein, the NIR‐II fluorescent nanoprobe NP‐Pd for in vivo ratiometric bioimaging of CO is developed. In the presence of CO, NP‐Pd exhibits responsive enhancement in absorption at 808 nm, which amplifies the fluorescence signal of down‐conversion nanoparticles (DCNP) at 1060 nm under 808 nm excitation, while the fluorescence signal of DCNP at 1525 nm under 980 nm excitation remains unchanged and serves as an internal standard. Through this orthogonally ratiometric fluorescence strategy, accurate CO bioimaging and precise diagnosis of acute liver injury diseases are achieved in the mouse model experiments, providing a novel tool for the in vivo detection of CO‐related diseases.
Interfacial Engineering of Nickel Oxide‐Perovskite Interface with Amino Acid Complexed NiO to Improve Perovskite Solar Cell Performance
Authors: Mann Dilpreet Singh , Thakur Sakshi , Sangale Sushil S. , Jeong Kwang‐Un , Kwon Sung‐Nam , Na Seok‐In 
Published: September 2024

AbstractThe interface between NiO and perovskite in inverted perovskite solar cells (PSCs) is a major factor that can limit device performance due to defects and inappropriate redox reactions, which cause nonradiative recombination and decrease in open‐circuit voltage (VOC). In the present study, a novel approach is used for the first time, where an amino acid (glycine (Gly), alanine (Ala), and aminobutyric acid (ABA))‐complexed NiO are used as interface modifiers to eliminate defect sites and hydroxyl groups from the surface of NiO. The Ala‐complexed NiO suppresses interfacial non‐radiative recombination, improves the perovskite layer quality and better energy band alignment with the perovskite, resulting in improved charge transfer and reduced recombination. The incorporation of the Ala‐complexed NiO leads to a PCE of 20.27% with enhanced stability under the conditions of ambient air, light soaking, and heating to 85 °C, as it retains over 82%, 85%, and 61% of its initial PCE after 1000, 500, and 350 h, respectively. The low‐temperature technique also leads to the fabrication of a NiO thin film that is suitable for flexible PSCs. The Ala‐complexed NiO is fabricated on the flexible substrate and achieved 17.12% efficiency while retaining 71% of initial PCE after 5,000 bending.
Tandem Oxidation Activation of Carbon for Enhanced Electrochemical Synthesis of H2O2: Unveiling the Role of Quinone Groups and Their Operando Derivatives
Authors: Zhao Dantong , Jiao Dongxu , Yi Lingya , Yu Yang , Zou Jiajia , Cui Xiaoqiang , Hu Weihua 
Published: September 2024

AbstractOxygen‐doped carbon materials show great promise to catalyze two‐electron oxygen reduction reaction (2e‐ORR) for electrochemical synthesis of hydrogen peroxide (H2O2), but the identification of the active sites is the subject of ongoing debate. In this study, a tandem oxidation strategy is developed to activate carbon black for achieving highly efficient electrochemical synthesis of H2O2. Acetylene black (AB) is processed with O2 plasma and subsequent electrochemical oxidation, resulting in a remarkable selectivity of >96% over a wide potential range, and a record‐setting high yield of >10 mol gcat−1 h−1 with good durability in gas diffusion electrode. Comprehensive characterizations and calculations revealed that the presence of abundant C═O groups at the edge sites positively correlated to and accounted for the excellent 2e‐ORR performance. Notably, the edge hydroquinone group formed from quinone under operando conditions, which is overlooked in previous research, is identified as the most active catalytic site.
Electrochemically Induced Nanoscale Stirring Boosts Functional Immobilization of Flavocytochrome P450 BM3 on Nanoporous Gold Electrodes
Authors: Hengge Elisabeth , Steyskal Eva‐Maria , Dennig Alexander , Nachtnebel Manfred , Fitzek Harald , Würschum Roland , Nidetzky Bernd 
Published: September 2024

AbstractEnzyme‐modified electrodes are core components of electrochemical biosensors for diagnostic and environmental analytics and have promising applications in bioelectrocatalysis. Despite huge research efforts spanning decades, design of enzyme electrodes for superior performance remains challenging. Nanoporous gold (npAu) represents advanced electrode material due to high surface‐to‐volume ratio, tunable porosity, and intrinsic redox activity, yet its coupling with enzyme catalysis is complex. Here, the study reports a flexible‐modular approach to modify npAu with functional enzymes by combined material and protein engineering and use a tailored assortment of surface and in‐solution methodologies for characterization. Self‐assembled monolayer (SAM) of mercaptoethanesulfonic acid primes the npAu surface for electrostatic adsorption of the target enzyme (flavocytochrome P450 BM3; CYT102A1) that is specially equipped with a cationic protein module for directed binding to anionic surfaces. Modulation of the SAM surface charge is achieved by electrochemistry. The electrode‐adsorbed enzyme retains well the activity (33%) and selectivity (complete) from in‐solution. Electrochemically triggered nanoscale stirring in the internal porous network of npAu‐SAM enhances speed (2.5‐fold) and yield (3.0‐fold) of the enzyme immobilization. Biocatalytic reaction is fueled from the electrode via regeneration of its reduced coenzyme (NADPH). Collectively, the study presents a modular design of npAu‐based enzyme electrode that can support flexible bioelectrochemistry applications.
Synergistic Catalysis of Rh Single‐Atom and Clusters Supported on TiO2 Nanosheet Array for Highly Efficient Removal of CO and NOx
Authors: Yang Liu , Wang Junchao , Liu Tingting , He Hanze , Li Xinyu , Zhang Xinglai , Li Jing , Li Song , Liu Baodan 
Published: September 2024

Developing an efficient catalyst is the key to selective catalytic reduction (SCR) of NOx by CO (CO‐SCR) to simultaneously address the pollution of toxic NOx and CO. Herein, a novel Rh/TiO2/Ti monolithic catalyst is designed and synthesized, featuring Rh species in the form of single atoms (Rh1) and clusters (Rhn). This catalyst overcomes the inhibitory effects of oxygen, achieving low‐temperature NO conversion. The investigation substantively contributes insights into the strategic manipulation of active metal components, emphasizing the potential of single‐atom/cluster catalysts to enhance efficiency. The Rh/TiO2/Ti catalyst has demonstrated exceptional catalytic efficacy, achieving 100% NO conversion at a low temperature of 190 °C in the presence of oxygen. Additionally, it exhibits remarkable stability and water resistance for practical applications. Moreover, comprehensive characterization confirms that Rh clusters and single‐atom sites play an important role in the selective adsorption of NO and CO molecules, promoting the formation of –N2O species and ultimately resulting in the complete conversion of NO and CO to N2 and CO2. This study not only provides valuable guidance for designing high‐performance CO‐SCR catalysts but also underscores the potential of single atoms/clusters catalytic systems in both fundamental research and industrial catalysis.
Unveiling the Anticarcinogenic Potential of Inula racemosa Hook. f. Root Extract Against DMBA‐Induced Mammary Tumour in Sprague Dawley Rats
Authors: Jaikaria Ankita , Kumar Rakesh , Asrani R. K. , Jamwal Smriti , Verma Abhishek , Santoshrao Joshi Gaurav , Bisen Harsh Krishnakumar , Patial Vikram , Sharma Dixit , Kumar Rohit , Kumar Adarsh , Patil R. D. 
Published: September 2024

ABSTRACTThe Himalayan plant Inula racemosa has medicinal properties and can be used to prevent or treat cancer. This is because it contains certain chemicals that are known to fight cancer cells with few or no side effects. I. racemosa has been used for this purpose for many years in traditional medicine and has shown promising results. The present study was crafted to explore the suppressive impacts on cellular proliferation of the root extract derived from I. racemosa via in vivo experimentation. I. racemosa (IR) root extract was tested at three different doses (100, 250, and 500 mg/Kg BW) for 18 weeks to assess its anti‐neoplastic activity against mammary tumors in female rats. The assessment included various parameters such as hematological and biochemical indices, tumor parameters, oxidative stress analysis, gross and histopathological lesion determination, Masson's trichrome staining, immunohistochemical expression of Ki‐67, MMP‐9, and VEGF in mammary gland tissues, and molecular docking. The chemopreventive action of IR root extract was demonstrated by the inhibition of tumor parameters (tumor size and tumor volume), minimum changes in the liver (ALT, AST, and ALP) and kidney enzymes (BUN and creatinine), declined lipid peroxidation activity, decline gross, and histological changes in mammary gland tumors, reduced expression of KI‐67, MMP‐9, and VEGF and maximum binding affinity of isoalantolactone with VEGF through molecular docking. The study suggests that the active constituents (isoalantolactone and alantolactone) of I. racemosa roots have anti‐neoplastic activity against mammary tumors, making them a valuable therapeutic regimen for the future.
Assessment of Shallow Wells of Public Importance in Selected Areas of Ekiti State Capital City, Ado Ekiti, Nigeria
Authors: Adeleke Adeyeye Joseph , Olabode Badmus Ganiyu , Toyin Omotoso 
Published: September 2024

ABSTRACTGroundwater is one of the main sources of public water supply all over the world. This study assessed hand‐dug wells of economic importance in selected areas of Ado Ekiti in relation to terrain characteristics. A total of 15 wells were randomly sampled with replicates from 5 densely populated parts of the study area. Water samples collected were processed and analyzed in the laboratory using established scientific methods. Results showed that physicochemical characteristics of the sampled groundwater compared favorably with the standard for drinking water, except for phosphate (PO43−) concentration, which was above the permissible limit of 0.1 mg/L. Moreover, while lead (Pb2+) was within the acceptable limits of 0.001 mg/L, cadmium (Cd2+) and iron (Fe2+) mean values of 0.01 and 16.723 mg/L were above the permissible limits of 0.003 and 0.03 mg/L, respectively. All the samples analyzed were found to contain total coliforms. Piper diagram indicates prevalence of chloride anion facies and a small portion of sulfate with no carbonate ions. There was no dominant water type among the cations. The chemical makeup mainly showed the prevalence of strong acids (Cl−) and slightly alkaline earth elements (Ca2+). The Schoeller plot suggests the dominance of calcium and elevated chloride concentrations in comparison to other ions. The water quality index indicated widespread pollution in most of the sampled water, showing that the shallow wells are not suitable for drinking purposes except in Adebayo and Erifun axis as expected. The study revealed terrain's influence and unprofessional construction of wells. A holistic water safety plan is imperative in the area and thus recommended.
Efficacy, safety and differential outcomes of immune‐chemotherapy with gemcitabine, cisplatin and durvalumab in patients with biliary tract cancers: A multicenter real world cohort
Authors: Mitzlaff Katharina , Kirstein Martha M. , Müller Christian , Venerito Marino , Olkus Alexander , Dill Michael T. , Weinmann Arndt , Kocheise Lorenz , Busch Alina , Schulze Kornelius , Allo Gabriel , Waldschmidt Dirk‐Thomas , Barsch Maryam , Bengsch Bertram , Quante Michael , Gonzalez‐Carmona Maria A. , Himmelsbach Vera , Finkelmeier Fabian , Kloeckner Roman , Schirmacher Peter , Marquardt Jens U. , Zimpel Carolin 
Published: September 2024

AbstractBackgroundCombined Immuno‐chemotherapy consisting of gemcitabine, cisplatin and the programmed death‐ligand one inhibitor durvalumab (GCD) is the new standard of care for patients with biliary tract cancers (BTC) based on positive results of the TOPAZ‐1 study.ObjectiveWe here evaluated the efficacy and safety of GCD for BTC in a German multicenter real‐world patient cohort.MethodsPatients with BTC treated with GCD from 9 German centers were included. Clinicopathological baseline parameters, overall survival (OS), response rate and adverse events (AEs) were retrospectively analyzed. The prognostic impact was determined by Kaplan–Meier analyses and Cox regression models.ResultsA total of 165 patients treated with GCD between 2021 and 2024 were included in the study. Median OS and median progression‐free survival were 14 months (95% CI 10.3–17.7) and 8 months (95% CI 6.8–9.2), respectively. The best overall response rate was 28.5% and disease control rate was 65.5%. While extrahepatic and intrahepatic BTC showed similar outcomes, mOS was significantly shorter in patients with gall bladder cancer (GB‐CA) with 9 months (95% CI 5.5–12.4; p = 0.02). In univariate analyses age ≥70 years, Eastern Cooperative Oncology Group (ECOG) performance status (PS) ≥1, status post cholecystectomy, GB‐CA and high baseline CRP values were significantly associated with OS. ECOG PS ≥ 1 and GB‐CA remained independent prognostic factors for OS in multivariable cox regression analysis. AEs have been reported in 130 patients (78.8%), including 149 grade 3–4 AEs (25.5%). One patient died of severe infectious pneumonia. Immune‐related (ir)AEs occurred in 17 patients (10.3%), including 9 grade 3–4 irAEs (2.2%), which led to treatment interruption in 4 patients.ConclusionsImmuno‐chemotherapy in patients with BTC was feasible, effective and safe in a real‐life scenario. Our results were comparable to the phase 3 clinical trial results (TOPAZ‐1). Reduced efficacy was noted in patients with GB‐CA and/or a reduced performance status that warrants further investigation.