bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of fullPIERS model for prediction of adverse outcomes among women with severe pre‐eclampsia
Authors: Almeida Silvana T. , Katz Leila , Coutinho Isabela , Amorim Melania M.R. 
Published: May 2017

AbstractObjectiveTo evaluate the accuracy of the Pre‐eclampsia Integrated Estimate of RiSk (fullPIERS) model for the prediction of complications among patients with severe pre‐eclampsia in northeastern Brazil.MethodsA retrospective study was conducted of data for all patients with severe pre‐eclampsia admitted to a center in Recife in 2014. The fullPIERS model was applied to calculate the predicted probability of complications. A receiver operating characteristic curve was constructed to determine the accuracy of the model, and the area under the curve was calculated. Multiple logistic regression analysis was performed.ResultsAmong 325 included women, 55 (16.9%) had one or more complication before discharge. The area under the curve was 0.72 (P<0.001), determining a cutoff point for fullPIERS probability of 1.7%. Sensitivity was 60.0% and specificity was 65.1%; the positive likelihood ratio was 1.72 and the negative likelihood ratio was 0.61. In multivariate analysis, the only variable that remained significantly associated with complications was a fullPIERS probability of more than 1.7% (odds ratio 5.87, 95% confidence interval 3.16–10.89; P<0.001).ConclusionThe fullPIERS model is a useful tool for predicting complications in women with severe pre‐eclampsia, with the cutoff point of 1.7%.
The Potential Value of the PI3K/Akt/mTOR Signaling Pathway for Assessing Prognosis in Cervical Cancer and as a Target for Therapy
Authors: Bahrami Afsane , Hasanzadeh Malihe , Hassanian Seyed Mahdi , ShahidSales Soodabeh , Ghayour‐Mobarhan Majid , Ferns Gordon A. , Avan Amir 
Published: May 2017

ABSTRACTCervical cancer is a common gynecological cancer and a leading cause of cancer‐related death in women globally. There is a need for the identification of prognostic and predictive biomarker for risk stratification. The phosphatidylinositol 3‐kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway is often dysregulated in cervical cancer, indicating that it may be a potential therapeutic target in the treatment of this malignancy, and could perhaps be used as a novel biomarker in the assessment of risk of developing cervical cancer. We aimed to provide an overview of the potential applications of the PI3K/Akt/mTOR pathway as biomarker for risk stratification, in predicting the prognosis of cervical cancer, and for developing new therapeutic approaches in patients with cervical cancer. J. Cell. Biochem. 118: 4163–4169, 2017. © 2017 Wiley Periodicals, Inc.
Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives
Authors: Li Fenfang , Li Qiao , Wu Shuanggen , Sun Danyu , Tan Zhijian 
Published: May 2017

AbstractBACKGROUNDThe aqueous two‐phase extraction (ATPE) based on organic solvent and inorganic salt was considered a promising method in the extraction, enrichment, and purification field. Ionic liquids (ILs) used as additives to the aqueous two‐phase system (ATPS) can improve the extraction yield.RESULTSIn this study, seven ILs were added to the ethanol/ammonium sulfate ATPS and used for the salting‐out extraction (SOE) of sinomenine from Sinomenium acutum. The IL [C2OHmim]FeCl4 was screened as the optimal additive, then the influencing factors IL amount, ethanol concentration, salt concentration, and temperature were investigated. The maximum extraction efficiency of 96.4% was obtained under the following conditions: 23.06 wt% ethanol concentration, 27.28 wt% (NH4)2SO4 concentration, 4.0 wt% [C2OHmim]FeCl4 and 40°C temperature. Moreover, the components in the SOE system can be recycled after extraction.CONCLUSIONThus, this ATPS had lower cost and higher extraction ability, and it is feasible to scale up using a small amount of ILs. This system can also be used in the extraction of some other important active compounds from natural plant resources. © 2017 Society of Chemical Industry
Ultrastructural preservation of tissues and their reaction to the infection with trichinella in the El Plomo mummy: Muscle fiber ultrastructure and trichinosis/mummy of the Cerro El Plomo
Authors: Héctor Rodríguez , Omar Espinoza‐Navarro , González Mercedes , Mario Castro 
Published: May 2017

AbstractThe El Plomo mummy was a pre‐Columbian Incan child who was found mummified in the Andes Mountains above an altitude of 17,700 feet. In the environment, natural mummification occurred due to low temperatures and strong winds. Dating measurements (relative dating) by experts from the National Museum of Natural History of Chile established that the mummified body corresponds the Inca period (1,450 to 1,500 AD). In 2003, the body was transferred to the University of Chile Medical School for exhaustive medical examination. Tissue samples from the right quadriceps muscle were extracted and fixed in glutaraldehyde and postfixed in osmium tetroxide to obtain ultrathin sections to be observed by transmission electron microscope. Images were recorded on photographic paper, digitalized and analyzed by experts on morphology. Results showed a preservation of cell boundaries in striated muscle cells, but specific subcellular organelles or contractile sarcomeric units (actin and myosin) were unable to be recognized. However, the classical ultrastructural morphology of the polypeptide collagen type I was preserved intact both in primary and secondary organization. Therefore, we concluded that the process of natural mummification by freezing and strong winds is capable of damaging the ultrastructure of muscle cells and preserving collagen type I intact.
Mobilization of glucose‐6‐phosphate from ferrihydrite by ligand‐promoted dissolution is higher than of orthophosphate
Authors: Goebel Marc-Oliver , Adams Friederike , Boy Jens , Guggenberger Georg , Mikutta Robert 
Published: May 2017

AbstractWe investigated glucose‐6‐phosphate (G6P) release from ferrihydrite by ligand‐promoted dissolution by oxalate, ascorbate, and desferrioxamine‐B in comparison to orthophosphate (OP). Overall, P release was poorly related to Fe dissolution. Initial release of G6P was higher as compared to OP, likely due to its preferential adsorption at outer surface sites caused by pore size effects. Our data suggest that G6P is potentially more bioavailable (i.e., mobilizable) than OP.
1H NMR study of the phase behavior of aqueous eutectic solutions using the inverse Laplace transform analysis of T2 relaxation
Authors: Baughman Jessi A. , Joseph Ray G. , Christina Szabo M. , Werling Jane O. , Rinaldi Peter L. 
Published: May 2017

High‐field 1H NMR T2 relaxation studies were used to characterize the changes in the physical phases of water, NaCl, and dextrose solutions over a temperature range of −65 to 15 °C. The data were analyzed with the inverse Laplace transform and with a linear fit to the logarithm of the time domain signal. Two liquid phases were detected for the NaCl and dextrose solutions at lower temperatures and assigned to low and high concentrated solution domains. The high concentrated solution domain was found to be present between −30 and −5 °C in the NaCl solution and between −55 and −5 °C in the dextrose solution. Copyright © 2017 John Wiley & Sons, Ltd.
Circulating selenoprotein P levels in relation to MRI‐derived body fat volumes, liver fat content, and metabolic disorders
Authors: di Giuseppe Romina , Koch Manja , Schlesinger Sabrina , Borggrefe Jan , Both Marcus , Müller Hans‐Peter , Kassubek Jan , Jacobs Gunnar , Nöthlings Ute , Lieb Wolfgang 
Published: May 2017

ObjectiveAssociation studies of selenoprotein P (SELENOP) with cardiometabolic traits in humans are relatively scarce and, in part, conflicting. A general population sample from Northern Germany was evaluated for cross‐sectional associations of circulating SELENOP concentrations with metabolic syndrome (MetS), total volumes of MRI‐determined visceral (VAT) and subcutaneous (SAT) abdominal adipose tissue, liver signal intensity, and fatty liver disease (FLD).MethodsNine hundred and five participants received comprehensive clinical and molecular phenotyping along with measurement of serum SELENOP; 584 individuals received MRI.ResultsMultivariable‐adjusted restricted cubic regression splines displayed statistically significant inverse relations of SELENOP levels with MetS, VAT, and SAT (P < 0.0001 for all). Compared with the second quartile of SELENOP distribution, the MetS odds ratios for the first, third, and fourth quartiles were 1.62 (95% confidence interval [CI]: 1.08‐2.43), 0.85 (95% CI: 0.57‐1.26), and 0.41 (95% CI: 0.27‐0.62), respectively. Furthermore, participants in the second, third, and fourth SELENOP quartiles had significantly lower VAT and SAT volumes as compared to those in the first biomarker quartile. A J‐shaped relation was observed for SELENOP levels and liver signal intensity/FLD (P = 0.01).ConclusionsThe findings suggest inverse associations of circulating SELENOP concentrations with several metabolic traits, to be further investigated in longitudinal studies.
The H, H2+, and HeH2+ systems confined by an impenetrable spheroidal cavity: Revisited study via the Lagrange‐mesh approach
Authors: Olivares‐Pilón Horacio , Cruz Salvador A. 
Published: May 2017

AbstractThe one electron systems H, H
, and HeH
confined by an impenetrable spheroidal cavity are revisited in the frame of the Lagrange‐mesh method. The Born–Oppenheimer approximation where the nuclei are clamped at the foci is considered. Benchmark results of the total energy are obtained as a function of the interfocal distance R and the eccentricity of the cavity
. Dipole oscillator strengths are calculated for the molecular ions H
and HeH
.
Bone Marrow Endothelial Progenitor Cells Are the Cellular Mediators of Pulmonary Hypertension in the Murine Monocrotaline Injury Model
Authors: Aliotta Jason M. , Pereira Mandy , Wen Sicheng , Dooner Mark S. , Del Tatto Michael , Papa Elaine , Cheng Yan , Goldberg Laura , Ventetuolo Corey E. , Liang Olin , Klinger James R. , Quesenberry Peter J. 
Published: May 2017

Abstract
The role of bone marrow (BM) cells in modulating pulmonary hypertensive responses is not well understood. Determine if BM-derived endothelial progenitor cells (EPCs) induce pulmonary hypertension (PH) and if this is attenuated by mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). Three BM populations were studied: (a) BM from vehicle and monocrotaline (MCT)-treated mice (PH induction), (b) BM from vehicle-, MCT-treated mice that received MSC-EV infusion after vehicle, MCT treatment (PH reversal, in vivo), (c) BM from vehicle-, MCT-treated mice cultured with MSC-EVs (PH reversal, in vitro). BM was separated into EPCs (sca-1+/c-kit+/VEGFR2+) and non-EPCs (sca-1-/c-kit-/VEGFR2-) and transplanted into healthy mice. Right ventricular (RV) hypertrophy was assessed by RV-to-left ventricle+septum (RV/LV+S) ratio and pulmonary vascular remodeling by blood vessel wall thickness-to-diameter (WT/D) ratio. EPCs but not non-EPCs from mice with MCT-induced PH (MCT-PH) increased RV/LV+S, WT/D ratios in healthy mice (PH induction). EPCs from MCT-PH mice treated with MSC-EVs did not increase RV/LV+S, WT/D ratios in healthy mice (PH reversal, in vivo). Similarly, EPCs from MCT-PH mice treated with MSC-EVs pre-transplantation did not increase RV/LV+S, WT/D ratios in healthy mice (PH reversal, in vitro). MSC-EV infusion reversed increases in BM-EPCs and increased lung tissue expression of EPC genes and their receptors/ligands in MCT-PH mice. These findings suggest that the pulmonary hypertensive effects of BM are mediated by EPCs and those MSC-EVs attenuate these effects. These findings provide new insights into the pathogenesis of PH and offer a potential target for development of novel PH therapies.
Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell-Derived Choroidal Endothelium
Authors: Songstad Allison E. , Worthington Kristan S. , Chirco Kathleen R. , Giacalone Joseph C. , Whitmore S. Scott , Anfinson Kristin R. , Ochoa Dalyz , Cranston Cathryn M. , Riker Megan J. , Neiman Maurine , Stone Edwin M. , Mullins Robert F. , Tucker Budd A. 
Published: May 2017

Abstract
Age-related macular degeneration (AMD) is a leading cause of irreversible blindness in the Western world. Although, the majority of stem cell research to date has focused on production of retinal pigment epithelial (RPE) and photoreceptor cells for the purpose of evaluating disease pathophysiology and cell replacement, there is strong evidence that the choroidal endothelial cells (CECs) that form the choriocapillaris vessels are the first to be lost in this disease. As such, to accurately evaluate disease pathophysiology and develop an effective treatment, production of patient-specific, stem cell-derived CECs will be required. In this study, we report for the first time a stepwise differentiation protocol suitable for generating human iPSC-derived CEC-like cells. RNA-seq analysis of the monkey CEC line, RF/6A, combined with two statistical screens allowed us to develop media comprised of various protein combinations. In both screens, connective tissue growth factor (CTGF) was identified as the key component required for driving CEC development. A second factor tumor necrosis factor (TNF)-related weak inducer of apoptosis receptor was also found to promote iPSC to CEC differentiation by inducing endogenous CTGF secretion. CTGF-driven iPSC-derived CEC-like cells formed capillary tube-like vascular networks, and expressed the EC-specific markers CD31, ICAM1, PLVAP, vWF, and the CEC-restricted marker CA4. In combination with RPE and photoreceptor cells, patient-specific iPSC derived CEC-like cells will enable scientists to accurately evaluate AMD pathophysiology and develop effective cell replacement therapies.
Tailoring of Hetero–oligophenylene Stabilized Nanohybrid Materials: Potential Tandem Photo‐Promoted Systems for C–C and C–X Bond Formation Reactions via C‐H Activation
Authors: Walia Preet Kamal , Kumar Manoj , Bhalla Vandana 
Published: May 2017

AbstractSupramolecular assemblies of the hetero‐oligophenylene derivative 3 served as reactors for the facile synthesis of ZnO and Pd NPs in mixed aqueous media. Unprecendentally, these assemblies provided a common platform for the generation of bimetallic Pd‐ZnO nanohybrid materials in aqueous media which served as efficient catalytic systems in C−C/C−X bond forming reactions. The freshly prepared zinc oxide nanoparticles showed high efficiency in (i) single pot three (in situ five) component reactions for the synthesis of tetrahydropyridine derivatives ii) photo‐promoted synthesis of propargylamines via A3‐coupling reaction. On the other hand, freshly prepared bimetallic Pd‐ZnO nanohybrid materials enabled efficient harvesting of visible light radiation to catalyze biaryl coupling and “green” Sonogashira coupling in aqueous media. Additionally, ZnO NPs and Pd NPs in combination with bimetallic Pd‐ZnO nanohybrid materials efficiently photocatalyzed the “one‐pot two‐step reactions” comprising Sonogashira cross coupling, reduction and three component reaction for the synthesis of tetrahydropyridine derivative in good yield.
Synthesis and Characterization of a New Class of Triazoles Containing Bicyclic Silaheterocycles
Authors: Wang Tengjiao , Cho Hyeon Mo , Lee Myong Euy 
Published: May 2017

AbstractA new class of bicyclic silaheterocycles, 6,6‐dimethyl‐2‐aryl‐6,7‐dihydro‐5H‐[1,2,4]triazolo[5,1‐b][1,3,5]thiazasilines, which could be developed as potential sila‐drugs. The reactions of several 3‐aryl‐5‐mercapto‐1,2,4‐triazoles with bis(chloromethyl)dimethylsilane under basic conditions gave high yields of bicyclic products. Reaction, product workup and isolation were streamlined without requiring an anhydrous solvent, inert gas or chromatographic purification. The products were structurally characterized by single‐crystal X‐ray diffraction.
Metal‐Free Alkyl(Aryl) Transfer‐Aromatization‐Alkylation Domino Approach: Facile Synthesis of Branched Hydroquinones from p‐Quinols and Diaryl Carbinols
Authors: Singh Pallavi , Peddinti Rama Krishna 
Published: May 2017

AbstractSimple but effective and eco‐friendly metal‐free alkyl(aryl) transfer‐aromatization‐alkylation domino (ATAAD) approach was demonstrated to synthesize branched hydroquinones in good to excellent yields. This high atom economy approach proceeded through molecular iodine‐catalyzed aromatization of p‐quinols followed by C–C bond formation with diaryl carbinols. The use of inexpensive catalyst and easily accessible starting materials rendered this clean method more exciting for wide substrate scope to afford symmetrical and unsymmetrical branched hydroquinones. Short reaction time, easy workup and gram‐scale preparation are the significant features of this hitherto unknown synthesis of the title compounds.
N‐Arylsulfonimides as Photoinitiators for Cationic Polymerization of Epoxy Sol‐Gel Materials
Authors: Torti Edoardo , Protti Stefano , Fagnoni Maurizio , Della Giustina Gioia 
Published: May 2017

AbstractN‐arylsulfonimides, a new class of non‐ionic photoacid generators (PAGs) able to release two equivalents of strong sulfonic acids per equivalent of PAG are employed in solid state polymerization within an epoxy based hybrid sol‐gel system. Thus, epoxy ring opening reactions were successfully induced by UV light exposure of sol‐gel films and the organic network progression was followed by infrared spectroscopy. Polymerization degrees reached with N‐arylsulfonimides were close to that obtained with a commercial PAG (4‐phenylthiophenyl)diphenylsulfonium triflate, DPST). Different micrometric patterns with satisfactory pattern definition were achieved by UV lithography of sol‐gel films containing a N‐arylsulfonimide. These new PAGs are therefore promising photoinitiators to be applied in photopolymerization processes and microelectronics.
Bulky 2,6‐Dibenzhydryl‐4‐methylaniline Derived Schiff Base Complexes of Pd(II), Cu(II) and Co(II) as Efficient Catalysts for Suzuki Coupling and Alcohol Oxidation Reactions
Authors: Saxena Priya , Murugavel Ramaswamy 
Published: May 2017

AbstractBulky 2,6‐dibenzhydryl‐4‐methylaniline reacts with 2‐hydroxybenzaldehyde or 2‐pyridinecaboxyaldehyde to form [N,O‐] and [N,N] donor ligands, 2‐(((2,6‐dibenzhydryl‐4‐methylphenyl)imino)methyl)phenol (L1) and N‐(2,6‐dibenzhydryl‐4‐methylphenyl)‐1‐(pyridin‐2‐yl)methanimine (L2), respectively. Schiff bases, L1 and L2 on treatment with metal precursors, MCl2 (M = Pd, Cu or Co) in toluene under reflux conditions afford mono‐ or dinuclear complexes [(L1‐H)2M] (M= Pd (1) and Cu (2)) or [L2(μ‐Cl)M]2 (M= Cu (3) and Co (4)). The ligands and complexes were structurally characterized through single crystal X‐ray diffraction and depending upon the type of compound, further characterized by spectroscopic (IR, Mass, NMR, EPR, UV‐Vis), mircroanalytical (CHN), thermal (TGA) and electrochemical (CV) techniques. Complex 1 was employed in Suzuki‐Miyaura coupling whereas complexes 2‐4 were studied for the oxidation of secondary alcohols. These were found to be efficient catalysts for the respective reaction systems.
Cellulose‐Derived Hollow Carbonaceous Nanospheres from Rice Husks as Anode for Lithium‐Ion Batteries with Enhanced Reversible Capacity and Cyclic Performance
Authors: Yu Kaifeng , Li Jian , Qi Hui , Liang Ce 
Published: May 2017

AbstractDisordered carbons are widely used as anode materials for lithium‐ion batteries due to their stable cycle performance, high specific capacity and negative redox potentials. Among them, as an environmental friendly renewable source, biomass has attracted much attention for its application in the preparation of disordered carbon. We report a novel carbonaceous material with interconnected hollow nanosphere structure which used cellulose extracted from rice husk (RH) as a precursor and prepared through hydrothermal carbonization and high temperature calcination. When used as anodes for lithium‐ion batteries, the first discharge specific capacity is as high as 1040 mAhg−1 at a rate of 0.2 C and the reversible specific capacity stabilized at 489 mAhg−1 after 100 cycles. The novel disordered nanostructure material displays superior Lithium‐ion battery performance with large reversible capacities, excellent cyclic performance, and outstanding rate capability, highlighting the importance of hollow interconnected structure for maximum utilization of electrochemically active for energy storage applications in high‐performance lithium‐ion batteries.
About P‐glycoprotein: a new drugable domain is emerging from structural data
Authors: Ferreira Ricardo J. , Bonito Cátia A. , Ferreira Maria José U. , dos Santos Daniel J.V.A. 
Published: May 2017

P‐glycoprotein (P‐gp) has been considered an important molecular target in the reversal of multidrug resistance (MDR). As such, the development of P‐gp modulators able to restore drug sensitivity in resistant cells is still considered one of the most promising strategies for overcoming MDR. Since the identification of the P‐gp's role in MDR, several studies have been performed in order to develop effective P‐gp modulators and understand the efflux mechanism. However, no efflux modulator is still clinically available for treating multidrug‐resistant cancers. Nevertheless, recent experimental studies suggest that MDR can be surpassed by targeting a specific region within the ABC transporter structure rather than the polyspecific drug‐binding pocket. This article will focus on the information available about this new target region and on a brief overview of which scaffolds would be suitable for modulating P‐gp at this new location. WIREs Comput Mol Sci 2017, 7:e1316. doi: 10.1002/wcms.1316This article is categorized under:

Structure and Mechanism > Computational Biochemistry and Biophysics
Quantification of epithelial cell proliferation, cell dynamics, and cell kinetics in vivo
Authors: Goodlad Robert A. 
Published: May 2017

The measurement of cell proliferation in vivo is usually carried out by the examination of static measures. These comprise the mitotic index or labeling indices using incorporation of DNA synthesis markers such as bromodeoxyuridine or tritiated thymidine, or intrinsic markers, such as Ki67 and proliferative cell nuclear antigen (PCNA). But static measures only provide a ‘snapshot’ of cell proliferation. Rate measures, including double labeling methods and the metaphase arrest method, can actually measure cell production rates but they are far less utilized at present. Transit times and migration rates can also be measured using pulse and chase labeling or by following the transit of labeled cells through the tissue. Simple indices of cell division can easily be confounded by concomitant changes in the compartment size and many alleged markers of proliferation have serious shortcomings, as the markers may be involved in multiple aspects of cell regulation. The complexities of studying proliferation in vivo are illustrated here with a focus on the gastrointestinal tract. Some of these methods can help elucidate the role of the stem cells and their relationship to label retaining cells. WIREs Dev Biol 2017, 6:e274. doi: 10.1002/wdev.274This article is categorized under:

Gene Expression and Transcriptional Hierarchies > Quantitative Methods and Models