bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in the psychosocial work characteristics and insulin resistance among Japanese male workers: a three‐year follow‐up study
Authors: Hino Ayako , Inoue Akiomi , Mafune Kosuke , Nakagawa Toru , Hayashi Takeshi , Hiro Hisanori 
Published: September 2016

ObjectiveThis study investigated the impact of changes in psychosocial work characteristics on insulin resistance (IR) among Japanese male workers.MethodsSubjects were 1,815 male workers who received a comprehensive health examination and requested measurement of their serum insulin level in Fiscal Years (FY) 2008 and 2011. Psychosocial work characteristics, including job demands, job control, and workplace social support (from supervisors and coworkers), were assessed in each of the job demands‐control and demand‐control‐support models. Psychosocial work characteristics were assessed by the Brief Job Stress Questionnaire. Changes in the psychosocial work characteristics were measured by creating a four‐category variable for each of the psychosocial work characteristics: (1) stable low group, (2) increased group, (3) decreased group, and (4) stable high group. We defined IR as a value of 2.5 or more on the homeostasis model assessment of insulin resistance (HOMA‐IR), or having a diagnosis of diabetes. A series of multiple logistic regression analyses were conducted.ResultsThe group experiencing a decrease in supervisor support had a significantly higher risk of having IR compared to the stable high group with an odds ratio (OR) of 2.44; 95% CI: 1.48‐4.02. After adjusting for covariates, this significant association was unchanged; the OR was 2.19; 95% CI: 1.23‐3.91. On the other hand, there was no significant association of changes in the psychosocial work characteristics, expect for decrease in supervisor support, with IR.ConclusionsA decrease in supervisor support was found to be an independent risk factor for worsening IR.
Safety of Oral Propranolol for the Treatment of Infantile Hemangioma: A Systematic Review
Authors: Léaute-Labrèze Christine , Boccara Olivia , Degrugillier-Chopinet Caroline , Mazereeuw-Hautier Juliette , Prey Sorilla , Lebbé Geneviève , Gautier Stéphanie , Ortis Valérie , Lafon Martine , Montagne Agnès , Delarue Alain , Voisard Jean-Jacques 
Published: September 2016

BACKGROUND AND OBJECTIVES:
Given the widespread use of propranolol in infantile hemangioma (IH) it was considered essential to perform a systematic review of its safety. The objectives of this review were to evaluate the safety profile of oral propranolol in the treatment of IH.

METHODS:
We searched Embase and Medline databases (2007–July 2014) and unpublished data from the manufacturer of Hemangiol/Hemangeol (marketed pediatric formulation of oral propranolol; Pierre Fabre Dermatologie, Lavaur, France). Selected studies included ≥10 patients treated with oral propranolol for IH and that either reported ≥1 adverse event or effect (AE) or planned to capture AEs. Data capture was standardized and extracted study design, demographic characteristics, IH characteristics, intervention, and safety outcomes. AEs were assigned a system organ class and preferred term.

RESULTS:
A total of 83 of 398 identified literature records met the inclusion criteria, covering 3766 propranolol-treated patients. The manufacturer’s data for 3 pooled clinical trials (435 propranolol-treated patients) and 1 Compassionate Use Program (1661 patients) were included. AE data were reported for 1945 of 5862 propranolol-treated patients. The most frequently reported AEs included a range of sleep disturbances, peripheral coldness, and agitation. The most serious AEs (atrioventricular block, bradycardia, hypotension, bronchospasm/bronchial hyperreactivity, and hypoglycemia-related seizures) were managed by decreasing doses or temporary/permanent discontinuation of propranolol. Limitations included the variety of included study designs; monitoring, collection, and reporting of AE data; small sample sizes for some articles; and the wide scope of review.

CONCLUSIONS:
Oral propranolol is well tolerated if appropriate pretreatment assessments and within-treatment monitoring are performed to exclude patients with contraindications and to minimize serious side effects during treatment.
Predicting Severe Pneumonia Outcomes in Children
Authors: Williams Derek J. , Zhu Yuwei , Grijalva Carlos G. , Self Wesley H. , Harrell Frank E. , Reed Carrie , Stockmann Chris , Arnold Sandra R. , Ampofo Krow K. , Anderson Evan J. , Bramley Anna M. , Wunderink Richard G. , McCullers Jonathan A. , Pavia Andrew T. , Jain Seema , Edwards Kathryn M. 
Published: September 2016

BACKGROUND:
Substantial morbidity and excessive care variation are seen with pediatric pneumonia. Accurate risk-stratification tools to guide clinical decision-making are needed.

METHODS:
We developed risk models to predict severe pneumonia outcomes in children (&lt;18 years) by using data from the Etiology of Pneumonia in the Community Study, a prospective study of community-acquired pneumonia hospitalizations conducted in 3 US cities from January 2010 to June 2012. In-hospital outcomes were organized into an ordinal severity scale encompassing severe (mechanical ventilation, shock, or death), moderate (intensive care admission only), and mild (non–intensive care hospitalization) outcomes. Twenty predictors, including patient, laboratory, and radiographic characteristics at presentation, were evaluated in 3 models: a full model included all 20 predictors, a reduced model included 10 predictors based on expert consensus, and an electronic health record (EHR) model included 9 predictors typically available as structured data within comprehensive EHRs. Ordinal regression was used for model development. Predictive accuracy was estimated by using discrimination (concordance index).

RESULTS:
Among the 2319 included children, 21% had a moderate or severe outcome (14% moderate, 7% severe). Each of the models accurately identified risk for moderate or severe pneumonia (concordance index across models 0.78–0.81). Age, vital signs, chest indrawing, and radiologic infiltrate pattern were the strongest predictors of severity. The reduced and EHR models retained most of the strongest predictors and performed as well as the full model.

CONCLUSIONS:
We created 3 risk models that accurately estimate risk for severe pneumonia in children. Their use holds the potential to improve care and outcomes.
Quantification of biofilm formation on silicone intranasal splints: An in vitro study
Authors: Pavlović Bojan , Božić Dragana D. , Milovanović Jovica , Jotić Ana , Djukić Vojko , Djukić Slobodanka , Konstantinović Neda , Ćirković Ivana 
Published: September 2016

Objectives:
Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints.

Methods:
Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations.

Results:
All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p < 0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p < 0.05).

Conclusion:
Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.
Comparison of five commercial nucleic acid extraction kits for the PCR-based detection of Burkholderia pseudomallei DNA in formalin-fixed, paraffin-embedded tissues
Authors: Obersteller Sonja , Neubauer Heinrich , Hagen Ralf Matthias , Frickmann Hagen 
Published: September 2016

The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR.
FFPE samples from organs of Burkholderia pseudomallei-infected Swiss mice were subjected to processing with five different extraction kits from QIAGEN (FFPE DNA Tissue Kit, EZ1 DNA Tissue Kit, DNA Mini Kit, DNA Blood Mini Kit, and FlexiGene DNA Kit) in combination with three different real-time PCRs targeting B. pseudomallei-specific sequences of varying length after 16 years of storage.
The EZ1 DNA Tissue Kit and the DNA Mini Kit scored best regarding the numbers of successful PCR reactions. In case of positive PCR, differences regarding the cycle-threshold (Ct) values were marginal.
The impact of the applied extraction kits on the reliability of PCR from FFPE material seems to be low. Interfering factors like the quality of the dewaxing procedure or the sample age appear more important than the selection of specialized FFPE kits.
EMBRYONIC AND LARVAL DEVELOPMENT OF A RED STRAIN OF THE EGYPTIAN AFRICAN CATFISH (Clarias gariepinus Burchell, 1822)
Authors: Iswanto Bambang , Imron Imron , Suprapto Rommy , Marnis Huria 
Published: September 2016

Egyptian African catfish is one of several African catfish (Clarias gariepinus) strains introduced to Indonesia. Several breeding activities using that strain in Sukamandi resulted in some individuals with redish-yellow body colour (a red strain). Biological informations related to aquacultural aspects of that red strain were still scarce. The present study aimed to elucidate the embryonic and larval developments of the red strain compared to those of the black (normal coloured) one, using the progenies obtained from artificial spawning of each red and black coloured brooders. Results of the present study revealed that embryonic developments of the red and black strains were similar. Their larval development were also quite similar, however, both larvae could be distinguished based on the pigmentation. The red strain larvae were golden-red-yellowish in colour, while the black strain larvae were greyish covered with dense melanophores. Total length of the red strain larvae tended to be lower than those of the black strain.
EFFECT OF DIFFERENT PROBIOTIC BACTERIA ON SURVIVAL RATE, GROWTH, AND PRODUCTION OF WHITELEG SHRIMP IN TRADITIONAL-PLUS TECHNOLOGY
Authors: Atmomarsono Muharijadi , Susianingsih Endang 
Published: September 2016

Instead of culturing tiger shrimp that is frequently burdened by mass mortality, whiteleg shrimp (Litopenaeus vannamei) is then considered as an alternative commodity in Indonesian brackishwater ponds. To prevent the whiteleg shrimp from diseases, different probiotic bacteria were tested in completely randomized design experiment using nine 250 m2 experimental ponds stocked with 10 PLs of whiteleg shrimp fry/m2. Three treatments were applied, namely A) alternate use of probiotic bacteria RICA-1, RICA-2, RICA-3; B) alternate use of probiotic bacteria RICA-4, RICA-5, RICA-3, and C) control (without probiotic bacteria); each with three replications. After 11-week application, the results showed that the best survival rate of whiteleg shrimp was achieved by treatment B 98.83%) and the best production was achieved by treatment A (23.52 kg/250 m2). However, there were no significant differences (P>0.05) among the three treatments tested for the shrimp survival rate. The whiteleg shrimp production in treatment A and B were signicantly better (P<0.05) than that in treatment C (control). These high shrimp production in treatment A and B were mainly caused by the capability of the applied probiotics in controlling some water quality variables and Vibrio numbers.
IMPROVED PRODUCTION OF TIGER SHRIMP (Penaeus monodon) THROUGH PROBIOTICS APPLICATION
Authors: Insan Irsyaphiani , Kontara Endhay Kusnendar , Dewi Raden Roro Sri Pudji Sinarni 
Published: September 2016

The study was carried out in Brebes District, the North coast of Java. Tiger shrimp farming in Indonesia, particularly in this area faced some problems which caused by improper pond preparation, disease, and low seed quality. Probiotic was applied in pond to solve this problem. The aim of this study was to evaluate the production of tiger shrimp in ponds with probiotic applications. Six experimental ponds (each measuring 0.5 ha) were selected of which three were probiotic ponds and three were controlled. Tiger shrimp postlarvae (PL-30) were stocked at density of four shrimps/m2. Tiger shrimps were reared for three months. Shrimps were fed by commercial pellet. In the first month, shrimp were fed about 7%-5% of the total biomass; in the second months, 3.5%-3% of the total biomass; and in the third month, 2.5%-2% of the total biomass. The treatments in this study were the application of probiotics with concentration of 3 mg/L that were given every five days and control (without probiotics). The results showed the rearing period was 92 ± 6 days in probiotic ponds and 76 ± 16 days in controlled pond. The shrimp in controlled pond should be harvest earlier caused by the high mortality. The average final weight was 16.2 ± 0.7 g in probiotic pond and 15.6 ± 1.9 g in controlled pond. The survival rate was 64.13 ± 12.63% in probiotic pond and 44.17 ± 14.15% in controlled pond. Production was 208 ± 46 kg/pond/cycle in probiotic pond and 123 ± 6 kg/pond/cycle in controlled pond. The result showed that probiotic plays an important role in maintaining water quality parameters and health management as well as increases the survival of shrimp.
IMMUNOGENICITY ASSESSMENT FOR DETERMINATION OF THE MOST POTENTIAL ISOLATES OF KOIHERPES VIRUS
Authors: Gardenia Lila , Koesharyani Isti , Mufidah Tatik 
Published: September 2016

Common carp and koi (Cyprinus carpio) are the main consumption fish commodity and ornamental fish in Indonesia. Diseases due to koiherpes virus (KHV) infection had caused a huge loss in both common carp and koi culture industries world-wide. This study was generated to select the most potential candidate from out of three koiherpes virus isolates based on their serological performances for vaccine development to control Koiherpes virus disease in koi. Virus collection, isolation, and propagation in cell culture have been conducted from common and koi samples originated from Banjarmasin, South Kalimantan; Takalar, South Sulawesi; Cirata Lake-Cianjur, West Java; Depok, West Java; Ciseeng, Bogor, West Java; and Purwokerto, Central Java. Determination of the causative agent of the disease was done by PCR (Polymerase Chain Reaction) assay and inoculation of filtrate homogenates from infected fish into cell culture. There are three koiherpes virus isolates (BJMN-1, BJMN-2, and SKBM) which are being successfully isolated, cultured and propagated in Koi Fin (KF-1) cell line. Calculation of tissue culture infection dose from each isolates were 104.55 TCID50/mL, 104.72 TCID50/mL, and 103.28 TCID50/mL. Immunoassay of the three isolates was conducted by Indirect-ELISA method. Polyclonal antibody was made by injecting each isolate to the experiment animals (rat/Rattus norwegicus strain Sprague Dawley). It was shown that all of three isolates have high level of immunogenicity, as seen from the absorbance values at 490 nm wavelength. BJMN-1 isolates have a higher potential for it’s ability to cross-react with the other isolates and provided the highest absorbance values. In future, all isolates can be used as KHV vaccine candidates to prevent KHV infection.
MORPHOLOGICAL CHARACTERIZATION OF THE AFRICAN CATFISH (Clarias gariepinus BURCHELL, 1822) STRAINS INTRODUCED TO INDONESIA
Authors: Iswanto Bambang , Imron Imron , Suprapto Rommy , Marnis Huria 
Published: September 2016

African catfish (Clarias gariepinus Burchell, 1822) has become a great important species in Indonesian aquaculture. Several strains of the African catfish have been introduced to Indonesia for aquaculture purposes, initiated by Dumbo strain from Taiwan in 1985, followed by Paiton strain from Thailand in 1998, then Egypt strain from Egypt in 2007, Masamo strain from Thailand in 2010 and later Kenya strain from Kenya in 2011. Since its introductions, there were no reports yet on their characterization studies. The present study was conducted to morphologically characterize the strains of African catfish introduced to Indonesia, i.e. Dumbo, Paiton, Egypt, Masamo, and Kenya strains. Morphometric and meristic data obtained were analyzed using Principal Component Analysis. Results of the morphometric characterization in the present study revealed that Dumbo, Paiton, Masamo, and Kenya strains were indistinguishable, while morphometric characteristic of Egypt strain was more or less different from those of the other strains. On the other hand, results of the meristic characterization suggested that meristic characteristics of all strains of the introduced African catfish were not different from each other. Therefore, to keep the genetic purity of those introduced strains, they should be properly maintained in isolated places.
HUBUNGAN SUBSTRAT DASAR PERAIRAN DENGAN KEHADIRAN RUMPUT LAUT ALAM DI PERAIRAN UJUNG GENTENG, SUKABUMI, JAWA BARAT
Authors: Johan Ofri , Erlania Erlania , Radiarta I Nyoman 
Published: September 2016

Keberadaan jenis-jenis rumput laut alam sangat terkait dengan kondisi habitat tempat tumbuhnya. Penelitian ini bertujuan untuk menganalisis hubungan distribusi jenis rumput laut alam dengan tipe substrat dasar perairan. Pengumpulan data lapangan telah dilakukan pada 146 titik pengamatan, menggunakan transek kuadrat berukuran 1 m x 1 m, yang disebar vertikal mulai dari garis pantai hingga ke arah tubir dengan jarak antar titik sekitar 50 m. Lokasi pengamatan dikelompokkan menjadi tiga kawasan berdasarkan jarakdari garis pantai dengan perbedaan karakteristik antar masing-masing kawasan tersebut, yaitu kawasan 1, 2, dan 3 dengan jarak 0-100 m, 100-300 m, dan > 300 m dari garis pantai. Hasil penelitian menunjukkan bahwa sebanyak 24 genus dari 34 spesies rumput laut yang termasuk dalam kelompok alga coklat, merah, dan hijau ditemukan pada ketiga kawasan dengan persentase total tutupan masing-masing 25,88%; 27,72%; dan 46,40%. Tutupan rumput laut tertinggi ditemukan pada kawasan 1, yang dicirikan oleh substrat pasir yang berasosiasi dengan lamun, dan diikuti oleh kawasan 2 dan 3. Hasil analisis diskriminan menunjukkan hubungan yang signifikan antara distribusi alga merah dengan tipe substrat pasir; alga coklat dengan tipe substrat batu karang, pecahan karang, dan karang hidup; sedangkan alga hijau tersebar hampir di seluruh kawasan dengan tipe substrat yang berbeda. Informasi keterkaitan substrat dan keberadaan jenis rumput laut sangat penting dalam mendukung pengembangan teknologi budidaya rumput laut.
Broad Detection of Alterations Predicted to Confer Lack of Benefit From EGFR Antibodies or Sensitivity to Targeted Therapy in Advanced Colorectal Cancer
Authors: Rankin Andrew , Klempner Samuel J. , Erlich Rachel , Sun James X. , Grothey Axel , Fakih Marwan , George Thomas J. , Lee Jeeyun , Ross Jeffrey S. , Stephens Philip J. , Miller Vincent A. , Ali Siraj M. , Schrock Alexa B. 
Published: September 2016

Abstract

Introduction.
A KRAS mutation represented the first genomic biomarker to predict lack of benefit from anti-epidermal growth factor receptor (EGFR) antibody therapy in advanced colorectal cancer (CRC). Expanded RAS testing has further refined the treatment approach, but understanding of genomic alterations underlying primary and acquired resistance is limited and further study is needed.

Materials and Methods.
We prospectively analyzed 4,422 clinical samples from patients with advanced CRC, using hybrid-capture based comprehensive genomic profiling (CGP) at the request of the individual treating physicians. Comparison with prior molecular testing results, when available, was performed to assess concordance.

Results.
We identified a RAS/RAF pathway mutation or amplification in 62% of cases, including samples harboring KRAS mutations outside of the codon 12/13 hotspot region in 6.4% of cases. Among cases with KRAS non-codon 12/13 alterations for which prior test results were available, 79 of 90 (88%) were not identified by focused testing. Of 1,644 RAS/RAF wild-type cases analyzed by CGP, 31% harbored a genomic alteration (GA) associated with resistance to anti-EGFR therapy in advanced CRC including mutations in PIK3CA, PTEN, EGFR, and ERBB2. We also identified other targetable GA, including novel kinase fusions, receptor tyrosine kinase amplification, activating point mutations, as well as microsatellite instability.

Conclusion.
Extended genomic profiling reliably detects alterations associated with lack of benefit to anti-EGFR therapy in advanced CRC, while simultaneously identifying alterations potentially important in guiding treatment. The use of CGP during the course of clinical care allows for the refined selection of appropriate targeted therapies and clinical trials, increasing the chance of clinical benefit and avoiding therapeutic futility.
Learning Analytics for Communities of Inquiry
Authors: Kovanovic Vitomir , Gasevic Dragan , Hatala Marek 
Published: September 2016

This paper describes a doctoral research that focuses on the development of a learning analytics framework for inquiry-based digital learning. This research builds on the the Community of Inquiry model (CoI) as a foundation commonly used in research and practice of digital learning and teaching. Specifically, the main contributions of this research are: i) the development of a novel text classification algorithm for (semi)automated message classification which enables for easier adoption of the CoI model, ii) understanding of the relationships between different socio-technological interactions and the dimensions of the CoI model.
Bracy Clark's 3D Horse Hoof Model
Authors: Boulton Clare 
Published: September 2016

<p><span>During the recent reordering of the RCVS headquarters a </span><span>6 inch square box was found containing a cardboard model and a folded piece of paper. Further</span><span> investigation revealed it was something rather exciting – the pasteboard model of a horse’s hoof. </span></p><p><span>Colleagues at the Royal Veterinary College kindly produced a 3D model of the Hoof, which you can view in the interactive video below. You can also read more about Bracy Clark's model hoof on our <a title="Horse hoof blog" href="http://www.rcvskblog.org/bracy_clarks_hoof_update/">Blog</a>.</span></p><p><span><br /></span></p><div class="sketchfab-embed-wrapper"><iframe src="https://sketchfab.com/models/931a2b20b3804a02a39e89bd588522b1/embed" frameborder="0" width="640" height="480"></iframe><p style="font-size: 13px; font-weight: normal; margin: 5px; color: #4a4a4a;"><a style="font-weight: bold; color: #1caad9;" href="https://sketchfab.com/models/931a2b20b3804a02a39e89bd588522b1?utm_medium=embed&amp;utm_source=website&amp;utm_campain=share-popup" target="_blank">Bracy Clark hoof model c1820</a> by <a style="font-weight: bold; color: #1caad9;" href="https://sketchfab.com/rcvsknowledge?utm_medium=embed&amp;utm_source=website&amp;utm_campain=share-popup" target="_blank">rcvsknowledge</a> on <a style="font-weight: bold; color: #1caad9;" href="https://sketchfab.com?utm_medium=embed&amp;utm_source=website&amp;utm_campain=share-popup" target="_blank">Sketchfab</a></p></div><br /> <img src="https://www.veterinaryevidence.org/rcvskmod/icons/oa-icon.jpg" alt="Open Access" />