bluuebunny/crossref_metadata_2025_split huggingface dataset:
A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence-guided surgery in newly diagnosed glioblastoma patients
Authors: Inogés Susana , Tejada Sonia , de Cerio Ascensión López-Díaz , Gállego Pérez-Larraya Jaime , Espinós Jaime , Idoate Miguel Angel , Domínguez Pablo Daniel , de Eulate Reyes García , Aristu Javier , Bendandi Maurizio , Pastor Fernando , Alonso Marta , Andreu Enrique , Cardoso Felipe Prósper , Valle Ricardo Díez 
Published: May 2017

Abstract
Background
Prognosis of patients with glioblastoma multiforme (GBM) remains dismal, with median overall survival (OS) of about 15 months. It is therefore crucial to search alternative strategies that improve these results obtained with conventional treatments. In this context, immunotherapy seems to be a promising therapeutic option. We hypothesized that the addition of tumor lysate-pulsed autologous dendritic cells (DCs) vaccination to maximal safe resection followed by radiotherapy and concomitant and adjuvant temozolomide could improve patients’ survival.

Methods
We conducted a phase-II clinical trial of autologous DCs vaccination in patients with newly diagnosed patients GBM who were candidates to complete or near complete resection. Candidates were finally included if residual tumor volume was lower than 1 cc on postoperative radiological examination. Autologous DCs were generated from peripheral blood monocytes and pulsed with autologous whole tumor lysate. The vaccination calendar started before radiotherapy and was continued during adjuvant chemotherapy. Progression free survival (PFS) and OS were analyzed with the Kaplan–Meier method. Immune response were assessed in blood samples obtained before each vaccines.

Results
Thirty-two consecutive patients were screened, one of which was a screening failure due to insufficient resection. Median age was 61 years (range 42–70). Karnofsky performance score (KPS) was 90–100 in 29%, 80 in 35.5% and 60–70 in 35.5% of cases. MGMT (O6-methylguanine-DNA-methyltransferase) promoter was methylated in 45.2% of patients. No severe adverse effects related to immunotherapy were registered. Median PFS was 12.7 months (CI 95% 7–16) and median OS was 23.4 months (95% CI 16–33.1). Increase in post-vaccination tumor specific immune response after vaccines (proliferation or cytokine production) was detected in 11/27 evaluated patients. No correlation between immune response and survival was found.

Conclusions
Our results suggest that the addition of tumor lysate-pulsed autologous DCs vaccination to tumor resection and combined radio-chemotherapy is feasible and safe. A multicenter randomized clinical trial is warranted to evaluate the potential survival benefit of this therapeutic approach.
Trial registration This phase-II trial was registered as EudraCT: 2009-009879-35 and ClinicalTrials.gov Identifier: NCT01006044 retrospectively registered
Phase I Study and Biomarker Analysis of Pyrotinib, a Novel Irreversible Pan-ErbB Receptor Tyrosine Kinase Inhibitor, in Patients With Human Epidermal Growth Factor Receptor 2–Positive Metastatic Breast Cancer
Authors: Ma Fei , Li Qiao , Chen Shanshan , Zhu Wenjie , Fan Ying , Wang Jiayu , Luo Yang , Xing Puyuan , Lan Bo , Li Meiying , Yi Zongbi , Cai Ruigang , Yuan Peng , Zhang Pin , Li Qing , Xu Binghe 
Published: May 2017

Purpose This phase I study assessed the safety, tolerability, pharmacokinetics, antitumor activity, and predictive biomarkers of pyrotinib, an irreversible pan-ErbB inhibitor, in patients with human epidermal growth factor receptor 2 (HER2)–positive metastatic breast cancer. Patients and Methods Pyrotinib was administered continuously, orally, once per day to patients who did not have prior exposure to tyrosine kinase inhibitors of HER2. Planned dose escalation was 80, 160, 240, 320, 400, and 480 mg. For pharmacokinetic analysis, timed blood samples were collected on day 1 and day 28. Next-generation sequencing was performed on circulating tumor DNA and genomic DNA from tumor samples. Results Thirty-eight patients were enrolled. The dose-limiting toxicity was grade 3 diarrhea, which occurred in two patients administered 480 mg of pyrotinib; thus, the maximum tolerated dose was 400 mg. Common pyrotinib-related adverse events included diarrhea (44.7% [17 of 38]), nausea (13.2% [five of 38]), oral ulceration (13.2% [five of 38]), asthenia (10.5% [four of 38]), and leukopenia (10.5% [four of 38]). The only grade 3 adverse event was diarrhea. Pharmacokinetic analyses indicated that pyrotinib exposure was dose dependent. The overall response rate was 50.0% (18 of 36), and the clinical benefit rate (complete response + partial response + stable disease ≥ 24 weeks) was 61.1% (22 of 36). The median progression-free survival was 35.4 weeks (95% CI, 23.3 to 40.0 weeks). The overall response rate was 83.3% (10 of 12) in trastuzumab-naive patients and 33.3% (eight of 24) in trastuzumab-pretreated patients. Preliminary results suggest that PIK3CA and TP53 mutations in circulating tumor DNA ( P = .013) rather than in archival tumor tissues ( P = .474) may predict the efficacy of pyrotinib. Conclusion Continuous once-per-day pyrotinib was well tolerated and demonstrated promising antitumor activity in HER2-positive patients with metastatic breast cancer. The maximum tolerated dose was established as 400 mg. Diarrhea was the dose-limiting toxicity. The promising antitumor activity and acceptable tolerability of pyrotinib warrant its further evaluation in a phase II study.
Replication of ribosomal DNA in Arabidopsis occurs both inside and outside the nucleolus during S phase progression
Authors: Dvořáčková Martina , Raposo Berta , Matula Petr , Fuchs Joerg , Schubert Veit , Peška Vratislav , Desvoyes Bénédicte , Gutierrez Crisanto , Fajkus Jiří 
Published: May 2017

ABSTRACT
Ribosomal RNA genes (rDNA) have been used as valuable experimental systems in numerous studies. Here, we focus on elucidating the spatiotemporal organisation of rDNA replication in Arabidopsis thaliana. To determine the subnuclear distribution of rDNA and the progression of its replication during the S phase, we apply 5-ethynyl-2′-deoxyuridine (EdU) labelling, fluorescence-activated cell sorting, fluorescence in situ hybridization and structured illumination microscopy. We show that rDNA is replicated inside and outside the nucleolus, where active transcription occurs at the same time. Nascent rDNA shows a maximum of nucleolar associations during early S phase. In addition to EdU patterns typical for early or late S phase, we describe two intermediate EdU profiles characteristic for mid S phase. Moreover, the use of lines containing mutations in the chromatin assembly factor-1 gene fas1 and wild-type progeny of fas1xfas2 crosses depleted of inactive copies allows for selective observation of the replication pattern of active rDNA. High-resolution data are presented, revealing the culmination of replication in the mid S phase in the nucleolus and its vicinity. Taken together, our results provide a detailed snapshot of replication of active and inactive rDNA during S phase progression.
Ecological Base-Conditioned Preparation of Dipeptides Using Unprotected α-Amino Acids Containing Hydrophilic Side Chains
Authors: Ezawa Tetsuya , Jung Seunghee , Kawashima Yuya , Noguchi Takuya , Imai Nobuyuki 
Published: May 2017

Abstract
The coupling reactions of 3-phenylpropanoic acid and N-carboxybenzyl α-amino acids with unprotected α-amino acids containing hydrophilic side chains such as aliphatic alcohol, aromatic alcohol, thiol, carboxylic acid, and amide afforded the corresponding amides in 66–96% yield without racemization via the corresponding mixed carbonic carboxylic anhydrides under basic conditions through an ecological green synthetic method.
Report of the General Committee: 17
Authors: McNeill John , Wilson Karen L. 
Published: May 2017

SummaryDecisions are reported on proposals and requests in Reports 61, 67, and 68 from the Nomenclature Committee for Vascular Plants, in Reports 10 and 11 from the Nomenclature Committee on Fossils, in Report 14 from the Nomenclature Committee for Algae and in Report 12 from the Nomenclature Committee for Bryophytes.
Temporary Incentives Change Daily Routines: Evidence from a Field Experiment on Singapore’s Subways
Authors: Yang Nan , Long Lim Yong 
Published: May 2017

We show that temporary incentives can lead to lasting changes in people’s daily routines through a field experiment. In randomized controlled trials on Singapore’s subways, commuters in the treatment group received a full rebate of their fares for 10 weeks if they exited fare gates before the start of morning peak-travel time. The objective of the fare promotion was to alleviate peak-time congestion by encouraging commuters to travel prepeak. This promotion resembles an actual public program implemented in Singapore in 2013. Using participants’ fare cards, we obtained a rich data set for 60 weeks’ worth of trips. A difference-in-differences analysis reveals that the effect of the free-trip offer was modest but robust during the promotional period: on average, trips were 5.5% more likely to end before the morning peak time. More encouragingly, the modest shift toward early travel strongly persisted even seven months after the end of the free-trip offer. This seemingly small shift could imply large financial savings on infrastructure investment. We find suggestive evidence that travel regularity and consistency during the promotion appear to be important for forging persistence. Data and the online appendix are available at https://doi.org/10.1287/mnsc.2017.2731 . This paper was accepted by Uri Gneezy, behavioral economics.
Does respite care address the needs of palliative care service users and carers? Their perspectives and experiences
Authors: Wolkowski Anna , Carr Susan M 
Published: May 2017

Aim: To establish whether respite care addresses the needs of palliative care service users and carers. Background: Respite care is often described in UK policy and guidance as a key need to providing support for this group and yet little is known about it and there is a lack of research to support its efficacy. Design: The approach was qualitative and the methodology was interpretive. The method used was constructivist grounded theory. Methods: Data collection was carried out by unstructured informal interview with three couples and two bereaved carers who had experienced hospice respite care. Results: Findings showed that respite care is valued by palliative care service users and carers although there are some fundamental tensions in service models which limit its potential. Conclusion: A reframing of respite care as an empathic response within a new palliative care approach is proposed. Within this, the centrality of the relationship is reinforced.
Thermal Analysis of porous fin with uniform magnetic field using Adomian decomposition Sumudu transform method
Authors: Patel Trushit , Meher Ramakanta 
Published: May 2017

AbstractIn this paper, we consider a Roseland approximation to radiate heat transfer, Darcy’s model to simulate the flow in porous media and finite-length fin with insulated tip to study the thermal performance and to predict the temperature distribution in a vertical isothermal surface. The energy balance equations of the porous fin with several temperature dependent properties are solved using the Adomian Decomposition Sumudu Transform Method (ADSTM). The effects of various thermophysical parameters, such as the convection-conduction parameter, Surface-ambient radiation parameter, Rayleigh numbers and Hartman number are determined. The results obtained from the ADSTM are further compared with the fourth-fifth order Runge-Kutta-Fehlberg method and Least Square Method(LSM) (Hoshyar et al. 2016 [
From Hades to the Stars: Empedocles on the Cosmic Habitats of Soul
Authors: Trépanier Simon 
Published: May 2017

καὶ πῶς τις ἀνάξει αὐτοὺς εἰς φῶς, ὥσπερ ἐξ Ἅιδου λέγονται δή τινες εἰς θεοὺς ἀνελθεῖν; Plato Republic 521c
This study reconstructs Empedocles’ eschatology and cosmology, arguing that they presuppose one another. Part one surveys body and soul in Empedocles and argues that the transmigrating daimon is a long-lived compound made of the elements air and fire. Part two shows that Empedocles situates our current life in Hades, then considers the testimonies concerning different cosmic levels in Empedocles and compares them with the afterlife schemes in Pindar’s Second Olympian Ode and Plato’s Phaedo myth. Part three offers a new edition of sectiond lines 5–10 of the Strasbourg papyrus of Empedocles that reinforces the connection between transmigration and different cosmic locations for souls. Part four reconstructs Empedocles’ cosmology, identifies three different levels or habitats of soul, and, more tentatively, suggests that Empedoclean “long-lived gods” are best understood as stars.
A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors
Authors: Imbert Paul RC , Louche Arthur , Luizet Jean‐Baptiste , Grandjean Teddy , Bigot Sarah , Wood Thomas E , Gagné Stéphanie , Blanco Amandine , Wunderley Lydia , Terradot Laurent , Woodman Philip , Garvis Steve , Filloux Alain , Guery Benoit , Salcedo Suzana P 
Published: May 2017

AbstractBacterial pathogens often subvert the innate immune system to establish a successful infection. The direct inhibition of downstream components of innate immune pathways is particularly well documented but how bacteria interfere with receptor proximal events is far less well understood. Here, we describe a Toll/interleukin 1 receptor (TIR) domain‐containing protein (PumA) of the multi‐drug resistant Pseudomonas aeruginosa PA7 strain. We found that PumA is essential for virulence and inhibits NF‐κB, a property transferable to non‐PumA strain PA14, suggesting no additional factors are needed for PumA function. The TIR domain is able to interact with the Toll‐like receptor (TLR) adaptors TIRAP and MyD88, as well as the ubiquitin‐associated protein 1 (UBAP1), a component of the endosomal‐sorting complex required for transport I (ESCRT‐I). These interactions are not spatially exclusive as we show UBAP1 can associate with MyD88, enhancing its plasma membrane localization. Combined targeting of UBAP1 and TLR adaptors by PumA impedes both cytokine and TLR receptor signalling, highlighting a novel strategy for innate immune evasion.
Birth Weight and Intelligence in Young Adulthood and Midlife
Authors: Flensborg-Madsen Trine , Mortensen Erik Lykke 
Published: May 2017

OBJECTIVES:
We examined the associations between birth weight and intelligence at 3 different adult ages.

METHODS:
The Copenhagen Perinatal Cohort is comprised of children born in Copenhagen from 1959 to 1961. Information on birth weight and ≥1 tests of intelligence was available for 4696 members of the cohort. Intelligence was assessed at a mean age of 19 years with the Børge Priens Prøve test, at age 28 years with the Wechsler Adult Intelligence Scale, and at age 50 years with the Intelligenz-Struktur-Test 2000 R.

RESULTS:
Birth weight was significantly associated with intelligence at all 3 follow-up assessments, with intelligence scores increasing across 4 birth weight categories and declining for the highest birth weight category. The adjusted differences between those in the &lt;2.5kg birth weight group and those in the 3.5 to 4.00kg group were &gt;5 IQ points at all 3 follow-up assessments, corresponding to one-third of a SD. The association was stable from young adulthood into midlife,and not weaker at age 50 years. Adjustment for potential confounding factors, including infant socioeconomic status and gestational age, did not dilute the associations, and associations with intelligence were evident across the normal birth weight range and so were not accounted for by low birth weight only.

CONCLUSIONS:
The association between birth weight and intelligence is stable from young adulthood into midlife. These long-term cognitive consequences may imply that even small shifts in the distribution of birth size, in normal-sized infants as well, may have a large impact at the population level.
A rare case of bilateral mandibular simple bone cyst and the importance of differential diagnosis
Authors: Falci Saulo Gabriel Moreira , Sales Késsia Nara Andrade , Da Silva Lorenna Vieira , Dos Santos Cássio Roberto Rocha 
Published: May 2017

Objective: To present a rare case of a mandibular bilateral simple bone cyst (SBC) with different radiological features and argue about the importance of the differential diagnosis.Case description: A 16-year-old boy was referred by his dentist to evaluate two radiolucent mandibular lesions. The diagnostic hypotheses were ameloblastoma or keratocystic odontogenic tumor to the left side and odontogenic cyst on the right side due radiologic features. In the left side, needle aspiration and biopsy, were performed. During surgical approach there was an empty space in the surgical cavity compatible with SBC. At this moment the diagnostic was made and the cavity was submitted to rigorous curettage. The right side was assessed after 4 months. To our surprise, it was found an empty cavity, as the left side. Six months later the first biopsy the panoramic radiograph showed completely bone neoformation on both sides. The patient is in radiographic follow-up for three years, without relapse.Conclusions: Bilateral SBC is a rare condition that can show different radiographic features. The diagnostic is defined at the surgery time. The radiographic features helps in diagnosis, however, should not be decisive. Surgical exploration should be done soon after radiographic diagnoses.
Reciprocating versus Rotary instruments: a review
Authors: Laurindo Flavia Villela , Figueiredo José Antonio Poli de 
Published: May 2017

Objective: Literature started to point out essential parameters involving reciprocating instruments through clinical and laboratory essays. Looking into apical debris extrusion, incidence of instrument breakage, canal centering ability, apical zipping, a myriad of information has become available. The aim of this review is to revisit the literature and compare reciprocal and continuous rotation techniques.Methods: A review of current literature may collect recent findings.Conclusion: Reciprocating systems are similar in some aspects in comparison to rotational systems, with regards to cleaning ability, centered preparations, cleaning ability, reduction of Enterococcus faecalis and dentine defects. On the other hand, being single use and enhanced resistance to fatigue, together with novel methods to treat the alloy may lead to the thought that reciprocal systems are an excellent aid to root canal preparation. However, more needs to be understood about this new era of instruments to verify, long term and especially in vivo, the success and failure when these instruments are used.
Influence of polymerization cycle in properties of acrylic resin polymerized by microwave energy
Authors: Collares Fabrício Mezzomo , Fortes Carmen Beatriz Borges , Leitune Vicente Castelo Branco , Rodrigues Stefani Becker , Samuel Susana Maria Werner , Petzhold César , Stefani Vater 
Published: May 2017

Objective: The purpose of this study was to evaluate the physicochemical properties of acrylic resin polymerized by microwave energy in short cycle of polymerization.Methods: Two brands (Classico and VIPI) and two cycles were evaluated (manufacturer cycle and short cycle). The characteristics and properties as degree of conversion, glass transition temperature, impact strength-Izod, Knoop hardness, swelling degree, soluble fraction, specific mass, water sorption and solubility were evaluated.Results: Glass transition temperature, hardness, specific mass, soluble fraction and solubility were statistically significant between cycles and brands (p<0.05). Water sorption showed no difference between cycles (p>0.05) and impact strength presented difference between brands in short cycle (p<0.05). Acrylic resin polymerized by microwave energy with manufacturer cycle presented no difference in physicochemical properties between evaluated brands. Conclusion: The short cycle of polymerization showed reduced properties in microwave acrylic resin when compared to manufacturer cycle. Manufacturer cycle of polymerization should be used to acrylic resin devices production.
A Gender-centered Perspective in the Institutional Analysis of Commons
Authors: Łapniewska Zofia 
Published: May 2017

People of communities, local societies or collectives work together to manage common-pooled resources (commons) without an external authority by combining multiple knowledge sources and ways of functioning. Since gender relations affect planning, production, well-being, and many other aspects of life, an in-depth look into the assumptions about commons might help avoid bias and distortion in disciplinary accounts.
This theoretical paper aims to present various ways and methods of including gender in the institutional analysis of communities and their actions related to the governing of commons. Therefore, it challenges gender relations within communities and introduces the meaning of socially-constructed institutions in the governing of common resources. Further, relying on my framework of institutional analysis and the given examples, I address the necessity of the inclusion of gender-centred perspective in deliberations on commons. This part provides a unique contribution to the studies on that subject. The main conclusion of this analysis is the need for a critical reflection of researchers, non-governmental organisations and members of communities on their institutions which can enhance or combat inequalities, thereby significantly impacting the achieved results.
Biopolítica, normalización y educación
Authors: Veiga-Neto Alfredo 
Published: May 2017

En este ensayo el autor expone dos razones por las cuales es posible establecer las relaciones entre biopolítica y educación: la primera hace referencia al hecho de que la educación es una práctica imprescindible par la acción biopolítica, lo que significa que la educación ocupó un lugar central en los procesos en los cuales el biopoder y los dispositivos de normalización se volvieron dominantes en las sociedades contemporáneas. La segunda razón, de orden práctico, tiene que ver con las posibilidades variadas y valiosas que las prácticas educativas -escolares y no escolares- nos ofrecen para observar y experimentar el funcionamiento del poder disciplinario, la biopolítica y los dispositivos de normalización y seguridad.
Semblanza y homenaje al profesor Rafael Ávila Penagos, Facultad de Educación
Authors: García Vera Nylza Offir 
Published: May 2017

He tenido la fortuna de leer varios de estos escritos y en mí resuenan aún muchos de sus conceptos y relaciones, perspectivas y apuestas, análisis y reflexiones, críticas y propuestas contenidas allí. Pero quizá en mi memoria resuenan en especial unas preguntas que se trazan al final de su libro, compilación de varias conferencias, clases y escritos, titulado Fundamentos de pedagogía. Allí se pregunta: “¿Educar a los jóvenes para cuál sociedad? ¿Formar maestros para cuál sociedad? ¿Gestionar escuelas para cuál sociedad?”. Aún no lo sabemos, pero abrigamos la esperanza de que nuestro país, nuestra universidad y nuestra facultad renazcan de sus propias cenizas, como el ave Fénix, porque es justamente en medio de las ruinas que es posible edificar y levantar algo nuevo y bueno, un inédito viable… algún día…