bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Ru(II) Polypyridyl Complex Bearing Bathocuproine Ligand is a Potent Chemotherapeutic Agent Against Chemically Induced Skin Cancer Model
Authors: Elias Maria George , Mehanna Stephanie , Nasser Selim , Daher Costantine F. , Khnayzer Rony S. 
Published: September 2024

AbstractRuthenium‐based compounds have emerged as prospective chemotherapeutic candidates with various mechanisms of action and minimal associated side effects compared to conventional metal‐based chemotherapeutics. The present study explores the chemotherapeutic potential of [Ru(bpy)2BC]Cl2 (where bpy = 2,2′‐bipyridine and BC = bathocuproine) or RuBC on a 7,12‐dimethylbenz[a]anthracene/12‐o‐tetradecanoylphorbol‐13‐acetate (DMBA/TPA) murine skin carcinogenesis model. RuBC is well tolerated up to 2.5 mg kg−1; no changes in body weight, behavior or serum biochemistry are observed. Following IP injections, the bioavailability of the complex is high in the plasma, which favors its accumulation in the organs. Efficacy studies demonstrated that RuBC has a significant anticancer activity by week 7 of treatment and a decrease in tumor size is observed by week 6 in all tested groups. Based on western blot analyses, apoptosis through the intrinsic pathway is suggested as the main mechanism of cell death. A downregulation of the MAPK pathway is also observed. The results indicate that RuBC is a multi‐mechanistic chemotherapeutic drug that has promising anticancer effects with significant potential for further investigation.
Tumor‐Resident Microbiota‐Based Risk Model Predicts Neoadjuvant Therapy Response of Locally Advanced Esophageal Squamous Cell Carcinoma Patients
Authors: Wu Hong , Liu Qianshi , Li Jingpei , Leng Xuefeng , He Yazhou , Liu Yiqiang , Zhang Xia , Ouyang Yujie , Liu Yang , Liang Wenhua , Xu Chuan 
Published: September 2024

AbstractFew predictive biomarkers exist for identifying patients who may benefit from neoadjuvant therapy (NAT). The intratumoral microbial composition is comprehensively profiled to predict the efficacy and prognosis of patients with esophageal squamous cell carcinoma (ESCC) who underwent NAT and curative esophagectomy. Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis is conducted to screen for the most closely related microbiota and develop a microbiota‐based risk prediction (MRP) model on the genera of TM7x, Sphingobacterium, and Prevotella. The predictive accuracy and prognostic value of the MRP model across multiple centers are validated. The MRP model demonstrates good predictive accuracy for therapeutic responses in the training, validation, and independent validation sets. The MRP model also predicts disease‐free survival (p = 0.00074 in the internal validation set and p = 0.0017 in the independent validation set) and overall survival (p = 0.00023 in the internal validation set and p = 0.11 in the independent validation set) of patients. The MRP‐plus model basing on MRP, tumor stage, and tumor size can also predict the patients who can benefit from NAT. In conclusion, the developed MRP and MRP‐plus models may function as promising biomarkers and prognostic indicators accessible at the time of diagnosis.
Ecological differentiation and sympatry of cryptic species in the Sphagnum magellanicum complex (Bryophyta)
Authors: Nieto‐Lugilde Marta , Nieto‐Lugilde Diego , Piatkowski Bryan , Duffy Aaron M. , Robinson Sean C. , Aguero Blanka , Schuette Scott , Wilkens Richard , Yavitt Joseph , Shaw A. Jonathan 
Published: September 2024

AbstractPremiseSphagnum magellanicum (Sphagnaceae, Bryophyta) has been considered to be a single semi‐cosmopolitan species, but recent molecular analyses have shown that it comprises a complex of at least seven reciprocally monophyletic groups, that are difficult or impossible to distinguish morphologically.MethodsNewly developed barcode markers and RADseq analyses were used to identify species among 808 samples from 119 sites. Molecular approaches were used to assess the geographic ranges of four North American species, the frequency at which they occur sympatrically, and ecological differentiation among them. Microhabitats were classified with regard to hydrology and shade. Hierarchical modelling of species communities was used to assess climate variation among the species. Climate niches were projected back to 22,000 years BP to assess the likelihood that the North American species had sympatric ranges during the late Pleistocene.ResultsThe species exhibited parallel morphological variation, making them extremely difficult to distinguish phenotypically. Two to three species frequently co‐occurred within peatlands. They had broadly overlapping microhabitat and climate niches. Barcode‐ versus RADseq‐based identifications were in conflict for 6% of the samples and always involved S. diabolicum vs. S. magniae.ConclusionsThese species co‐occur within peatlands at scales that could permit interbreeding, yet they remain largely distinct genetically and phylogenetically. The four cryptic species exhibited distinct geographic and ecological patterns. Conflicting identifications from barcode vs. RADseq analyses for S. diabolicum versus S. magniae could reflect incomplete speciation or hybridization. They comprise a valuable study system for additional work on climate adaptation.
Highly Stable Propane Dehydrogenation on a Self‐Supporting Single‐Component Zn2SiO4 Catalyst
Authors: Liu Zhaohui , Mao Min , Shu Tie , Cheng Qingpeng , Liu Dong , Wang Jianjian , Zhao Yun , Liu Lingmei , Han Yu 
Published: September 2024

AbstractCurrent industrial propane dehydrogenation (PDH) processes predominantly use either toxic Cr‐based or expensive Pt‐based catalysts, necessitating urgent exploration for alternatives. Herein, we present Zn2SiO4, an easily prepared, cost‐effective material, as a highly efficient and stable catalyst for PDH. Uniquely, Zn2SiO4 nanocrystals do not require dispersion on support materials, commonly needed for catalytic active oxide clusters, but function as a self‐supporting catalyst instead. During the reaction‘s induction period, surface Zn species on the Zn2SiO4 crystal reduce to coordinately unsaturated ZnOx single sites, serving as highly active catalytic centers. The Zn2SiO4 catalyst demonstrates a stable performance over 200 hours of PDH operation at 550 °C. We further find that introducing a minuscule amount of CO2 into the propane feed significantly extends the catalyst lifespan to over 2000 hours. This enhancement arises from the special role of CO2 in facilitating the removal of strongly adsorbed H*, preventing the complete reduction of ZnOx. After prolonged reaction, the activity of Zn2SiO4 can be fully restored by etching the surface layer to expose fresh Zn species, available throughout the crystals. The combination of CO2introduction and catalytic site regeneration strategies is expected to enable a year‐long PDH operation using a single batch of Zn2SiO4 catalyst.
Template‐assisted Flexible‐to‐rigid Transition of Peptides in Head‐to‐tail Self‐polymerization Enables Sequence‐controllable and Post‐modifiable Peptide Nanofibers
Authors: Chen Tianzi , Liu Yin , Gao Zhanshan , Gao Yue , Chen Haijin , Ye Haonan , Luo Qiuhao , Wang Kefeng , Wu Dongdong 
Published: September 2024

AbstractPeptide‐based nanofibers are promising materials for many essential applications and can be generalized into two categories, self‐assembling peptide nanofibers (SAPNs) and poly(amino acid) nanofibers (PAANs). Non‐covalent SAPNs are sequence‐controllable, but poorly stable and not suitable for post‐modification. While covalent PAANs are post‐modifiable, however, their sequences are either monotonic or undefined. The nanofibers obtained by head‐to‐tail covalent coupling polymerization of sequence‐known peptides, which we call series‐connected peptide nanofibers (SCPNs), promise to have the advantages of both SAPNs and PAANs, but they are barely reported. The undesired backbiting effect during the head‐to‐tail polymerization is one of the possible challenges. Here, we present a template‐assisted strategy to trigger the flexible‐to‐rigid transition of peptide units, which can avoid the backbiting effect and enable consecutive intermolecular polymerization of peptides to produce desired sequence‐controlled covalent SCPNs. SCPNs are highly stable and can function as excellent parent materials for various post‐processing to create diverse hierarchical materials independent of the peptide sequence. Moreover, SCPNs allow for the display of predetermined functional groups at regular intervals along the nanofibers by pre‐modification of the initial peptide sequence. SCPNs represent a new category of peptide‐based nanofibers with outstanding performances and vast potential.
Mechanical and physical properties of flexible polyurethane foam filled with waste tire material recycles
Authors: Nezili Y. , El Aboudi I. , He D. , Mdarhri A. , Brosseau C. , Zaghrioui M. , Chartier T. , Ghorbal A. , Arfi R. Ben , Bai J. 
Published: September 2024

AbstractIn this work we use ground tire rubber (GTR) powder obtained by grinding worn tire treads as reinforcer agent in flexible polyurethane (PU). Characterization of the microstructure of the as‐received powder is achieved using a series of standard techniques including scanning electron microscopy (SEM), granulometry‐laser, Fourier transform infrared spectroscopy (FTIR), and x‐ray diffraction (XRD). To have complementary physical information the composition and thermal characteristics of the GTR powder, thermogravimetry analysis (TGA) is also performed. The set of these preliminary characterizations shows that the GTR powder particles can be used as reinforcing fillers. For the purpose of good compatibility with the PU matrix, the GTR powder is subjected to chemical treatments for reducing the impurities on the powder particles and to create functional groups at their surface. Subsequently, a series of GTR/PU composite samples are prepared with different weight fractions of GTR using free rising foam method. The GTR/PU composites are then characterized to assess the effect of the GTR content and their chemically pre‐treatment on thermal stability, compression mechanical behavior as well as sound attenuation properties. Collectively, these results indicate a significant improvement of both thermal and mechanical properties of the GTR/PU composites compared to the pristine PU matrix. Furthermore, it is also emphasized that the sound absorption response shows a significant shift of the maximum of the absorption coefficient toward lower frequencies resulting from simultaneous increase in air‐flow resistivity and tortuosity which can have great potential application in the field of underwater acoustics. The effects of chemical treatments and GTR amount are also discussed. It is also shown that the results show improvement when H2O2 solvent is used in comparison with NaOH, and the optimal properties are reached for PU samples containing 20 wt% of GTR whatever the pre‐treatment is.
Combining Photogrammetry and Subsurface Geophysics to Improve Historical Knowledge of Romanesque Churches in Normandy, France: Case Study of the Notre‐Dame‐du‐Val Chapel
Authors: Fauchard Cyrille , Aillaud Laure , Legrand Astrid , Guilbert Vincent , Ledun Cyril , Beaucamp Bruno , Antoine Raphael 
Published: September 2024

ABSTRACTThis study presents the results of aerial and geophysical measurements carried out on the Notre‐Dame‐du‐Val chapel in Sotteville‐sur‐Mer (Normandy, France), a former leprosarium dating from the 15th century; the chapel is now deconsecrated and has been showing signs of ageing in recent years. Restoration work is planned, and geophysical investigations of the area around the chapel have been commissioned. In this article, we propose a robust methodology combining aerial and terrestrial measurements in the visible range with surface prospecting methods. Compiling all the measurements within a perfectly georeferenced 3D model allows the joint analysis of the results of different physical measurement methods to provide unexpected architectural and archaeological information. Photos were taken from the ground and using a drone to build photogrammetric models of the interior and exterior of the chapel. Ground‐penetrating radar (GPR) and electrical resistivity tomography (ERT) were the two survey methods deployed in the area surrounding the chapel. The geophysical measurements clearly reveal traces of apses—which have now disappeared—at the crossing of what would have been the building's transept, which match up with the filled‐in openings that are present. The existence of these apses can only be assumed from inside the chapel. The resistivity anomalies are perfectly correlated with the radar anomalies and allow new hypotheses to be formulated about the original structure of the chapel. Finally, mapping the local geology of the surroundings based on a geophysical survey provides crucial information about the history of the church's construction. Bringing this unknown architectural element to light and carrying out precise mapping of the local geology surrounding the chapel constitute a major breakthrough, as this will make it possible to improve our knowledge of the history of the chapel, in particular its origins, through research based on archaeological surveys.
Compartmentalized signaling in the soma: Coordination of electrical and protein kinase A signaling at neuronal ER‐plasma membrane junctions
Authors: Vierra Nicholas C. 
Published: September 2024

AbstractNeuronal information processing depends on converting membrane depolarizations into compartmentalized biochemical signals that can modify neuronal activity and structure. However, our understanding of how neurons translate electrical signals into specific biochemical responses remains limited, especially in the soma where gene expression and ion channel function are crucial for neuronal activity. Here, I emphasize the importance of physically compartmentalizing action potential‐triggered biochemical reactions within the soma. Emerging evidence suggests that somatic endoplasmic reticulum–plasma membrane (ER‐PM) junctions are specialized organelles that coordinate electrical and biochemical signaling. The juxtaposition of ion channels and signaling proteins at a prominent subset of these sites enables compartmentalized calcium and cAMP‐dependent protein kinase (PKA) signaling. I explore the hypothesis that these PKA‐containing ER‐PM junctions serve as critical sites for translating membrane depolarizations into PKA signals and identify key gaps in knowledge of the assembly, regulation, and neurobiological functions of this somatic signaling system.
Scaling up climate change finance in Ghana: The role of religious institutions
Authors: Mintah Kelvin Omari , Ahenkan Albert , Bawole Justice Nyigmah , Nakouwo Solomon Nborkan 
Published: September 2024

AbstractThe uncertainty surrounding the possibility of generating sufficient funds from international donors and other public financial resources to support climate action calls for the identification of alternative sources of climate finance options. Ensuring a reliable stream of funds to drive climate action requires the identification of diverse funding channels that can supplement existing ones. Also, in light of the proactive approach required to combat climate change, overreliance on a singular funding source could hinder the achievement of climate‐related goals. This is because the existing sources of funding from the central government, local governments, and international agencies remain insufficient to meet the perceived cost of climate change in Ghana. Through a qualitative in‐depth interview, empirical data was drawn from religious institutions to identify their contribution toward climate finance. The study revealed that religious institutions play a significant role in climate finance due to their advocacy capacity, financial resources, and extensive networks. Funding from religious institutions has also been channeled into stock adaptation projects and public education, and this is evident in the areas of agriculture, waste, energy, water, and the support rendered to SMEs in general. Besides, their engagement in climate finance is also motivated by ethical, theological, and moral considerations. The study, therefore, argues that funding from religious institutions toward climate change adaptation and mitigation initiatives should be seen as a complementary source of funding to climate finance.
Optimizing cryopreservation strategies for scalable cell therapies: A comprehensive review with insights from iPSC‐derived therapies
Authors: Dobruskin Michael , Toner Geoffrey , Kander Ronald 
Published: September 2024

AbstractOff‐the‐shelf cell therapies hold significant curative potential for conditions, such as Parkinson's disease and heart failure. However, these therapies face unique cryopreservation challenges, especially when novel routes of administration, such as intracerebral or epicardial injection, require cryopreservation media that are safe for direct post‐thaw administration. Current practices often involve post‐thaw washing to remove dimethyl sulfoxide (Me2SO), a cytotoxic cryoprotective agent, which complicates the development and clinical translation of off‐the‐shelf therapies. To overcome these obstacles, there is a critical need to explore Me2SO‐free cryopreservation methods. While such methods typically yield suboptimal post‐thaw viability with conventional slow‐freeze protocols, optimizing freezing profiles offers a promising strategy to enhance their performance. This comprehensive review examines the latest advancements in cryopreservation techniques across various cell therapy platforms, with a specific case study of iPSC‐derived therapies used to illustrate the scalability challenges. By identifying key thermodynamic and biochemical phenomena that occur during freezing, this review aims to identify cell‐type independent approaches to improve the efficiency and efficacy of cryopreservation strategies, thereby supporting the widespread adoption and clinical success of off‐the‐shelf cell therapies.
A rare case of perianal alveolar rhabdomyosarcoma
Authors: Taha Anas , Maeky Amjad , Wentzler Larissa , Taha‐Mehlitz Stephanie , Rosenberg Robert , Honaker Michael D. 
Published: September 2024

Key Clinical MessagePerianal alveolar rhabdomyosarcoma is a rare sarcoma that requires a high index of suspicion along with tissue biopsy for accurate diagnosis. Successful treatment, even in the setting of recurrence, requires a multidisciplinary approach.
Reexamination of doctoral‐level counselor educators’ journal article publication norms
Authors: Hatchett Gregory T. 
Published: September 2024

AbstractThis study reexamined the journal article publication norms of counselor educators employed in doctoral programs for the years 2017–2022. Compared to earlier studies, overall publication norms for this population declined but increased for counselor educators with traditional academic ranks. Four variables were useful for predicting publication counts.
Evaluation of Bi‐reforming Performance of Bi‐disperse Catalyst in a Packed Bed Reactor
Authors: Zhu Yaochen , Wang Shuai , Zhang Qinghong 
Published: September 2024

AbstractBi‐reforming hydrogen production has its potential in the reduction of greenhouse gas emissions. In this work, methane bi‐reforming process in a packed bed reactor using bi‐disperse catalyst particles is numerically investigated via a particle‐resolved modeling. The impacts of macropore fraction, porosity and macropore size on temperature and reaction rate distribution in the bed are evaluated. The results demonstrate that there exists a peak of the maximum temperature difference in the bed with the catalyst macropore fraction. Increasing the macropore fraction of the catalyst can weaken the non‐uniformity of coke formation in the bed. The increase in the macropore size of the catalyst particle can promote the hydrogen production, especially when the macropore size of particle is smaller.
Electrochemical Impedance Spectroscopy as a Characterization Method for Enzymatic Fuel Cell Bioanodes
Authors: Torrigino Federica , Nagel Marcel , Hartmann Martin , Herkendell Katharina 
Published: September 2024

AbstractEnzymatic fuel cells (EFCs) offer renewable energy conversion via highly selective electrode reactions using enzymes as natural catalysts even under mild conditions. Electrochemical impedance spectroscopy (EIS) is a valuable tool for evaluating EFC performance, providing insights into substrate mass transport, enzyme kinetics, and electrode stability. Despite its acknowledged importance, the use of EIS coupled with distribution of relaxation times (DRT) analysis in EFCs research is limited. Our study addresses this gap by employing EIS and DRT analysis to investigate enzyme‐based anodic processes, focusing on the bioelectrocatalytic oxidation of glucose catalyzed by glucose oxidase (GOx). Through careful variation of multiple parameters, it was possible to identify three distinct regions in the DRT plot. Each region has been subsequently associated with a key anodic process. The first region (R1) is associated with high‐frequency phenomena occurring at the electrodes, primarily due to ionic conduction in the electrolyte. Intermediate‐frequency processes are associated to charge transfer kinetics in region 2 (R2). Region 3 (R3) is linked to diffusion processes occurring at low frequencies. This thorough examination offers an insight into the functioning of enzymatic bioelectrodes, which in turn drives improvements in the design and components of biofuel cells to increase their power output.
Surface Nanostructuring of Copper Using Fluoride and Chloride
Authors: Pascual‐Llorens Vicente , Serra Ramos Albert , Mazaira‐Couce Pedro , Escudero‐Escribano María , Sebastián‐Pascual Paula 
Published: September 2024

AbstractCopper is an active electrocatalyst for various energy conversion reactions, but its performance depends on the structure of the active surface sites. In this work, we propose a simple strategy to tailor both the roughness and the active site's geometry of copper. To modify the surface of copper, we oxidize and reduce a copper polycrystalline electrode in 0.1 M solutions containing both sodium fluoride and sodium chloride with different chloride/fluoride molar ratios: (0.1‐x) M NaF+x M NaCl. To address the anion effect on the changes in surface geometry, we recorded the voltammetric fingerprints of the modified electrodes using lead underpotential deposition (UPD). The voltammetric analysis suggested that while chloride induces (n10) sites, fluoride promotes an increase in the active surface area and the growth of low‐coordinated sites with (110) or (111) geometry. Solutions containing both fluoride and chloride anions induced (n10) motifs covered by nanometric clusters, as observed by scanning electron microscopy, forming a highly defect‐rich surface. Our work provides a direct link between electrochemical response and ex‐situ structural characterization, and compares, in detail, the effect of chloride and fluoride on the surface nanostructuring of copper.