bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D super-resolution imaging of PSD95 reveals an abundance of diffuse protein supercomplexes in the mouse brain
Authors: Daly Sam , Bulovaite Edita , Handa Anoushka , Morris Katie , Muresan Leila , Adams Candace , Kaizuka Takeshi , Kitching Alexandre , Spark Alexander , Chant Gregory , O’Holleran Kevin , Grant Seth G. N. , Horrocks Mathew H. , Lee Steven F. 
Published: October 2024

AbstractPSD95 is an abundant scaffolding protein that assembles multiprotein complexes controlling synaptic physiology and behavior. Confocal microscopy has previously shown that PSD95 is enriched in the postsynaptic terminals of excitatory synapses and 2D super-resolution microscopy further revealed that it forms nanoclusters. In this study, we utilized 3D super-resolution microscopy to examine the nanoarchitecture of PSD95 in the mouse brain, characterizing over 8 million molecules. While we were able to identify structural subtypes previously reported, imaging in 3D allowed us to classify these with higher accuracy. Furthermore, 3D super-resolution microscopy enabled the quantification of protein levels, revealing an abundance of PSD95 molecules existed outside of synapses as a diffuse population of supercomplexes, containing multiple copies of PSD95. Further analysis of the supercomplexes containing two units identified two populations: one that had PSD95 molecules separated by 39 ± 2 nm, and a second with a separation of 94 ± 27 nm. These results suggest that PSD95 supercomplexes containing multiple protein copies assemble outside the synapse and then integrate into the synapse to form a supramolecular nanocluster architecture.
Individual G-quadruplex targeting with ligand-functionalized CRISPR-Cas9 uncovers transcriptional-dependent functional responses
Authors: Nuccio Sabrina Pia , Cadoni Enrico , Nikoloudaki Roxani , Galli Silvia , Ler An-Jie , Maher Thomas E. , Fan Ella , Liu Ling Sum , Fullenkamp Christopher R. , Schneekloth John S. , Di Antonio Marco 
Published: October 2024

AbstractThe development of selective ligands to target DNA G-quadruplexes (G4s) has been pivotal in revealing their role in transcriptional regulation. However, most of the ligands described to date lack intra-G4 selectivity, severely limiting their potential for uncovering the biological function of individual G4s across the genome. To overcome these limitations, we developed ATENA (Approach to Target Exact Nucleic Acid alternative structures). ATENA relies on the chemical modification of established G4-ligands to enable their conjugation onto a catalytically inactive Cas9 protein (dCas9) using HaloTag, allowing for the targeting of individual G4s in living cells. We have systematically screened the length of the PEG-linkers connecting the G4-ligands to the HaloTag and sgRNA sequences to attain optimal G4 engagement bothin vitroand in cells. Using optimized conditions, we leveraged ATENA to demonstrate how the selective targeting of the well-studied G4 in the promoter of the oncogenec-MYCsuppresses its transcription exclusively from the P1 promoter. We also show that positioning ligands in the proximity of regulatory elements suppressesc-MYCtranscription in a G4-independent manner, highlighting the importance of appropriate design to measure genuine G4-mediated transcriptional changes. We also demonstrate that selective targeting of a G4 in thePVT1promoter can either stimulate or repress its transcription depending on the type of G4-ligand used, indicating that functional responses associated with G4-stabilization can highly depend on the type of ligand used. We further harnessed ATENA to study transcriptional perturbation associated with cell-specific G4s, revealing that the functional responses associated with these structures are tightly linked with the expression levels of the targeted gene. Our study provides critical insights into G4-based therapeutic design, offering an innovative platform to investigate G4 biology with high precision.
Collectiveβ-lactam resistance inEscherichia colidue toβ-lactamase release upon cell death
Authors: Gross Rotem , Mungan Muhittin , Das Suman G. , Yüksel Melih , Maier Berenike , Bollenbach Tobias , Krug Joachim , de Visser J. Arjan G.M. 
Published: October 2024

AbstractCollective antibiotic resistance occurs when populations of bacteria survive antibiotic treatments that are lethal to individual bacteria, which affects the efficacy of drug therapies. Several mechanisms may lead to collective resistance, including the production of drug-degrading enzymes. Here, we integrate experiments with mathematical modeling to understand the collective survival ofEscherichia colichallenged with cefotaxime. We observe complex dynamics, involving initial biomass growth due to filamentation, followed by decline and subsequently growth recovery. We show that production of AmpC, a chromosomalβ-lactamase, is responsible for cefotaxime degradation, allowing the resumption of cell division in surviving filaments. Our model suggests that the release of AmpC via cell lysis accelerates antibiotic clearance, and does so particularly in strains with low cell-wall permeability that privatize periplasmic cefotaxime hydrolysis. Our findings support the hypothesis of enhanced survival ofβ-lactamase-producing bacterial populations via altruistic cell death.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder
Authors: Ghoochani Ali , Heiby Julia C. , Rawat Eshaan S. , Medoh Uche N. , Di Fraia Domenico , Dong Wentao , Gastou Marc , Nyame Kwamina , Laqtom Nouf N. , Gomez-Ospina Natalia , Ori Alessandro , Abu-Remaileh Monther 
Published: October 2024

AbstractMutations in lysosomal genes cause neurodegeneration and neurological lysosomal storage disorders (LSDs). Despite their essential role in brain homeostasis, the cell-type-specific composition and function of lysosomes remain poorly understood. Here, we report a quantitative protein atlas of the lysosome from mouse neurons, astrocytes, oligodendrocytes, and microglia. We identify dozens of novel lysosomal proteins and reveal the diversity of the lysosomal composition across brain cell types. Notably, we discovered SLC45A1, mutations in which cause a monogenic neurological disease, as a neuron-specific lysosomal protein. Loss of SLC45A1 causes lysosomal dysfunction in vitro and in vivo. Mechanistically, SLC45A1 plays a dual role in lysosomal sugar transport and stabilization of V1 subunits of the V-ATPase. SLC45A1 deficiency depletes the V1 subunits, elevates lysosomal pH, and disrupts iron homeostasis causing mitochondrial dysfunction. Altogether, our work redefines SLC45A1-associated disease as a LSD and establishes a comprehensive map to study lysosome biology at cell-type resolution in the brain and its implications for neurodegeneration.
Shaping the mechanical properties of a gelatin hydrogel interface via amination
Authors: Adorno Génesis Ríos , Timmer Kyle B. , Chang Raul A. Sun Han , Shi Jiachun , Rogers Simon A. , Harley Brendan A. C. 
Published: October 2024

ABSTRACTInjuries to musculoskeletal interfaces, such as the tendon-to-bone insertion of the rotator cuff, present significant physiological and clinical challenges for repair due to complex gradients of structure, composition, and cellularity. Advances in interface tissue engineering require stratified biomaterials able to both provide local instructive signals to support multiple tissue phenotypes while also reducing the risk of strain concentrations and failure at the transition between dissimilar materials. Here, we describe adaptation of a thiolated gelatin (Gel-SH) hydrogel via selective amination of carboxylic acid subunits on the gelatin backbone. The magnitude and kinetics of HRP-mediated primary crosslinking and carbodiimide-mediated secondary crosslinking reactions can be tuned through amination and thiolation of carboxylic acid subunits on the gelatin backbone. We also show that a stratified biomaterial comprised of mineralized (bone-mimetic) and non-mineralized (tendon-mimetic) collagen scaffold compartments linked by an aminated Gel-SH hydrogel demonstrate improved mechanical performance and reduced strain concentrations. Together, these results highlight significant mechanical advantages that can be derived from modifying the gelatin macromer via controlled amination and thiolation and suggest an avenue for tuning the mechanical performance of hydrogel interfaces within stratified biomaterials.
Immune cells promote paralytic disease in mice infected with enterovirus D68
Authors: Woods Acevedo Mikal A. , Lan Jie , Maya Sarah , Jones Jennifer E. , Williams John V. , Freeman Megan Culler , Dermody Terence S. 
Published: October 2024

AbstractEnterovirus D68 (EV-D68) is associated with acute flaccid myelitis (AFM), a poliomyelitis-like illness causing paralysis in young children. However, mechanisms of paralysis are unclear, and antiviral therapies are lacking. To better understand EV-D68 disease, we inoculated newborn mice intracranially to assess viral tropism, virulence, and immune responses. Wild-type (WT) mice inoculated intracranially with a neurovirulent strain of EV-D68 showed infection of spinal cord neurons and developed paralysis. Spinal tissue from infected mice revealed increased levels of chemokines, inflammatory monocytes, macrophages, and T cells relative to controls, suggesting that immune cell infiltration influences pathogenesis. To define the contribution of cytokine-mediated immune cell recruitment to disease, we inoculated mice lacking CCR2, a receptor for several EV-D68-upregulated cytokines, or RAG1, which is required for lymphocyte maturation. WT,Ccr2-/-, andRag1-/-mice had comparable viral titers in spinal tissue. However,Ccr2-/-andRag1-/-mice had significantly less paralysis relative to WT mice. Consistent with impaired T cell recruitment to sites of infection inCcr2-/-andRag1-/-mice, antibody-mediated depletion of CD4+or CD8+T cells from WT mice diminished paralysis. These results indicate that immune cell recruitment to the spinal cord promotes EV-D68-associated paralysis and illuminate new targets for therapeutic intervention.Graphical Abstract
Moving impedance profiles make one-way, spectrum-reshaping mirrors
Authors: Li Mingjie , Horsley S. A. R. 
Published: October 2024

We find a set of exact solutions to Maxwell's equations in spacetime-varying materials, where the refractive index is constant, while the impedance exhibits effective motion, i.e., it is a function of x−vt. We find that waves copropagating with the modulation are not reflected within the material, while counterpropagating waves are continually reflected by the changing impedance. For a finite section of such a material, we find analogs of transmission resonances, where specially shaped eigenpulses enter without reflection. We also find that there is a strong asymmetry in reflection from the medium when the impedance modulation is small but rapid, the material reflecting strongly from one side and negligibly from the other. Unlike stationary media, the spectrum of the reflected wave can be significantly different from the incident one.

Published by the American Physical Society
2024
Exploring users' fluidity and adaptation in virtual worlds: metaverse features and interaction of awe and realism
Authors: Arghashi Vahideh 
Published: October 2024

PurposeMetaverse technology has attracted much attention in many contexts, including industry, education, marketing and business. Some recent studies have focused on qualitative studies based on the actual definition of the metaverse. However, practical research related to metaverse platforms remains in its infancy. This study provides actionable insights into the determinants of metaverse adoption by using perceived fluidity.Design/methodology/approachA two-stage structural equation modeling (SEM) approach and Hayes’ Macro approach are used to examine the proposed hypotheses.FindingsResults show that technology features (e.g. real-time rendering, interactivity and immersion) increase users’ perceived fluidity, which in turn leads to positive intentions to use the metaverse. A high level of perceived realism is not an advantage for metaverse technology and plays a negative moderating role in this mechanism. The interaction of awe with technological features can enhance the negative moderating effects of realism.Originality/valueThis study pioneers the examination of perceived fluidity as a key determinant of metaverse adoption, offering a novel perspective beyond traditional factors. It uniquely identifies the paradoxical role of perceived realism, demonstrating its potential negative impact on user experience. In addition, the research highlights the reinforcing effect of awe on this relationship.
Guest editorial: Women and Gulf Cities. The transformative impact of women influencing the architectural and urban developments of the gulf region
Authors: Ottmann Daniela A. , Grichting Solder Anna , Hempel Adina 
Published: October 2024

PurposeWith this special edition, “Women and Gulf Cities”, we collect scholarly contributions to the ongoing discourse on the impact of women on architectural and urban developments in the Gulf Cooperation Council (GCC).Design/methodology/approachThe discussions offered in this issue highlight the various implications, ranging from gender-inclusive urban planning, educational and professional development, sustainability and heritage to global frameworks of innovative and practical solutions for achieving inclusive and integrated urban practices that promote the full and equal participation of women in architecture and city-making.FindingsThe increasing involvement of women in these sectors play a pivotal part in their development and success. It can also be recognised as essential for promoting individual and family well-being, fostering social cohesion and driving economic development. Women play essential roles in sustainable solutions, evolving green economy and sustainable development. Moreover, collaborations initiated by women in the Arab region are instrumental in advancing sustainable development to pave the way towards a sustainable future.Originality/valueThis special edition offers a comprehensive exploration of various facets, ranging from gender-sensitive urban design (GSUD) to the educational and professional development of women in architecture and design, particularly in the Gulf region. The originality of this research lies in its focus on the multifaceted and integral role of women in shaping the architectural and urban landscape of the Gulf region, providing valuable insights into the challenges and opportunities for GSUD and the potential for creating more inclusive, equitable and sustainable built environments in the Gulf.
Is there innovation management of emotions or just the commodification of happiness? A sentiment analysis of happiness apps
Authors: Mojica Manuel , Palos-Sanchez Pedro R. , Cabanas Edgar 
Published: October 2024

PurposeThe aim of this research is to gain insight into the emotional impact and cognitive evaluations that these applications have on users from their own perspective.Design/methodology/approachSentiment analysis methodology is used to analyze users’ comments and ratings, assess perceived benefits and determine the emotional impact these applications have on them.FindingsThe results suggest a benefit for happiness scholars, professionals, users and organizations focused on employee well-being and provide valuable insights into the evolving landscape of happiness applications. While the study relies on publicly available user data, it complements, but does not replace, market research or product evaluation conducted by app developers and companies.Originality/valueAmong the myriad channels disseminating the assumption that the good life is attainable through personal means and psychological know-how, smartphone applications have emerged as accessible, cost-effective and attractive tools to help users become happier and alleviate feelings of distress and discomfort.
An assessment of whether educated non-researcher audiences understand how to reuse research data
Authors: Wu Yejun , Bai Rujiang , Wang Fang 
Published: October 2024

Purpose
The purpose of this study is to assess whether educated non-researcher audiences understand how to reuse research data stored in a data repository.

Design/methodology/approach
A total of 44 participants in two user studies were asked to study a data set accessed from re3data.org. The participants were non-researcher audiences of the disciplines of the selected data sets. They were asked to figure out whether they understood how to reuse a data set after reading all the metadata or contextual information about the data set.

Findings
Most participants reported that they figured out how to reuse the data, although their self-reports can be an overestimated assessment. However, the participants understand how to reuse a data set either numerically or statistically significantly worse than what the data set is, how it was collected or created and its purpose. Data set type tends to play a role in understanding how to reuse data sets and the purpose of data sets. Participants reported that unless a data set is self-explanatory, instructions on data set reuse and the purpose of data set were necessary for understanding how to reuse data set. However, because data reuse requires domain knowledge and data processing skills, some non-researcher audiences who lack domain knowledge and data processing skills may not understand how to reuse the data set in any way.

Research limitations/implications
This study’s findings enrich the theoretical framework of data sharing and reuse by expanding the necessary information to be included in data documentation to support non-researchers’ data reuse. The findings of the study complement previous literature.

Practical implications
This study extended previous literature by suggesting detailed data reuse instructions be included in data documentation if data producers and data curators wish to support educated non-researchers’ data reuse. This study’s findings enable policymakers of research data management (RDM) to formulate guidelines for supporting non-researchers’ data reuse. If data curators need to work with data producers to prepare the instructions on data reuse for non-researcher audiences, they probably need computing and data processing skills. This has implications for Library and Information Science schools to educate data librarians.

Originality/value
The research question is original because non-researcher audiences in the context of RDM have not been studied before. This study extended previous literature by suggesting detailed data reuse instructions be included in data documentation if data curators and data producers and data curators wish to support educated non-researchers’ data reuse. This study’s findings enable policymakers of RDM to formulate guidelines for supporting non-researchers’ data reuse.
Collaborative interviewing of eyewitnesses: a field study
Authors: van Rosmalen Eva A.J. , Vredeveldt Annelies 
Published: October 2024

Purpose
When eyewitnesses talk to each other after witnessing a crime, they can contaminate each other’s memory. However, laboratory research shows that collaborative interviewing can also result in correction of mistakes and retrieval of more new information. The aim of this study is to examine whether these laboratory findings would generalise to real police interviews in The Netherlands. Because little is known about which interviewing techniques Dutch police detectives use, the secondary aim was to examine how Dutch detectives approach individual and collaborative eyewitness interviews.

Design/methodology/approach
In a field study, witnesses of serious incidents (e.g. police shooting) were interviewed individually and then collaboratively by real investigators, resulting in 15 interviews of 1–2 h each from five witness pairs (5,534 details in total). Transcripts were coded for detail type, forensic relevance, verifiability, retrieval strategies and interviewing techniques. Results were described using both quantitative descriptive data and a qualitative analysis of interview excerpts.

Findings
On average, collaborative interviews resulted in 131 new details, over half of which were considered highly relevant to the police investigation. Interview excerpts demonstrated how content-focused retrieval strategies (acknowledgements, repetitions, restatements, elaborations) can elicit new and highly relevant details. Interviewers mostly asked clarifying questions and equal numbers of open, closed and yes/no questions, but rarely allowed for uninterrupted free recall. Interviewers asked a higher proportion of open questions during collaborative interviews than during individual interviews.

Research limitations/implications
Limitations included the small sample size and lack of a control condition.

Originality/value
To the best of the authors’ knowledge, this paper is the first to investigate the effectiveness and feasibility of the Collaborative Eyewitness Interview in real-world settings.
Determining intention to visit halal destinations from the perspective of the push-pull-mooring framework
Authors: Maminirina Fenitra Rakotoarisoa , Hati Sri Rahayu Hijrah , Khan Ghazala , Setyowardhani Hapsari , Daryanti Sri , Ramayah Thurasamy 
Published: October 2024

PurposeThe rapid growth of halal tourism over the past decade has made it a lucrative market, with destinations competing for Muslim travellers. This study examines the factors influencing tourists' intention to visit halal tourism destinations in Indonesia, using the push-pull-mooring (PPM) model to understand this phenomenon.Design/methodology/approachData were collected using a self-administered questionnaire. A total of 328 usable responses were used to analyse the data. The proposed conceptual framework was tested using covariant-based structural equation modelling (CB-SEM).FindingsResults indicate that push, pull and mooring motives positively influence visit intention towards halal tourism destinations. Among these motives, novelty seeking, escapism, halal-friendly destination performance, halal attributes of the destination, religiosity and subjective norms were the most critical factors that improved visit intention. Furthermore, the moderating role of mooring factors was also established.Practical implicationsThe study makes several strategic suggestions for promoting halal tourism destinations. These include highlighting the destination’s halal index factor to attract Muslim tourists and develop the industry market.Originality/valueResearch in halal tourism is still emerging, and this study aims to enhance our understanding of Muslim travellers. Using the PPM framework, it provides valuable insights into Muslim tourists' intentions to visit halal destinations.
Collusion or supervision? Between auditor and audit firm – tax planning’s perspective
Authors: Hung Wen-Jye , Shu Pei-Gi , Ruan Yi-Yin , Wang Yamin 
Published: October 2024

Purpose
This study aims to investigate the impact of auditor industry specialization (AIS) on clients’ tax planning at the audit firm level and individual auditor level, respectively.

Design/methodology/approach
The study’s sample consists of 44,637 firm-year observations of Chinese firms listed on the Shenzhen and Shanghai Stock Exchanges during the period from 2002–2020. The data are collected from the Taiwan Economic Journal. Panel regression is used to test hypotheses. Additionally, a two-stage least squares model is used to address concerns about possible endogeneity.

Findings
The relationship between tax planning and AIS is significantly positive at the audit firm level, while it is significantly negative at the individual auditor level.

Originality/value
The authors use manually collected data to investigate the distinct impacts of two AIS metrics on tax planning: the number of clients and the scale of clients.