bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Toxoplasma gondii Rhoptry Kinome Is Essential for Chronic Infection
Authors: Fox Barbara A. , Rommereim Leah M. , Guevara Rebekah B. , Falla Alejandra , Hortua Triana Miryam Andrea , Sun Yanbo , Bzik David J. 
Published: May 2016

ABSTRACT  Ingestion of the obligate intracellular protozoan parasiteToxoplasma gondiicauses an acute infection that leads to chronic infection of the host. To facilitate the acute phase of the infection,T. gondiimanipulates the host response by secreting rhoptry organelle proteins (ROPs) into host cells during its invasion. A few key ROP proteins with signatures of kinases or pseudokinases (ROPKs) act as virulence factors that enhance parasite survival against host gamma interferon-stimulated innate immunity. However, the roles of these and other ROPK proteins in establishing chronic infection have not been tested. Here, we deleted 26ROPKgene loci encoding 31 unique ROPK proteins of type IIT. gondiiand show that numerous ROPK proteins influence the development of chronic infection. Cyst burdens were increased in the Δrop16knockout strain or moderately reduced in 11ROPKknockout strains. In contrast, deletion ofROP5,ROP17,ROP18,ROP35, orROP38/29/19(ROP38,ROP29, andROP19) severely reduced cyst burdens. Δrop5and Δrop18knockout strains were less resistant to host immunity-related GTPases (IRGs) and exhibited >100-fold-reduced virulence. ROP18 kinase activity and association with the parasitophorous vacuole membrane were necessary for resistance to host IRGs. The Δrop17strain exhibited a >12-fold defect in virulence; however, virulence was not affected in the Δrop35or Δrop38/29/19strain. Resistance to host IRGs was not affected in the Δrop17, Δrop35, or Δrop38/29/19strain. Collectively, these findings provide the first definitive evidence that the type IIT. gondiiROPK proteome functions as virulence factors and facilitates additional mechanisms of host manipulation that are essential for chronic infection and transmission ofT. gondii.IMPORTANCEReactivation of chronicToxoplasma gondiiinfection in individuals with weakened immune systems causes severe toxoplasmosis. Existing treatments for toxoplasmosis are complicated by adverse reactions to chemotherapy. Understanding key parasite molecules required for chronic infection provides new insights into potential mechanisms that can interrupt parasite survival or persistence in the host. This study reveals that key secreted rhoptry molecules are used by the parasite to establish chronic infection of the host. Certain rhoptry proteins were found to be critical virulence factors that resist innate immunity, while other rhoptry proteins were found to influence chronic infection without affecting virulence. This study reveals that rhoptry proteins utilize multiple mechanisms of host manipulation to establish chronic infection of the host. Targeted disruption of parasite rhoptry proteins involved in these biological processes opens new avenues to interfere with chronic infection with the goal to either eliminate chronic infection or to prevent recrudescent infections.
Geology and geologic history of the Moscow-Pullman basin, Idaho and Washington, from late Grande Ronde to late Saddle Mountains time
Authors: Bush John H. , Garwood Dean L. , Dunlap Pamela 
Published: May 2016

Abstract
The Moscow-Pullman basin, located on the eastern margin of the Columbia River flood basalt province, consists of a subsurface mosaic of interlayered Miocene sediments and lava flows of the Imnaha, Grande Ronde, Wanapum, and Saddle Mountains Basalts of the Columbia River Basalt Group. This sequence is ~1800 ft (550 m) thick in the east around Moscow, Idaho, and exceeds 2300 ft (700 m) in the west at Pullman, Washington. Most flows entered from the west into a topographic low, partially surrounded by steep mountainous terrain. These flows caused a rapid rise in base level and deposition of immature sediments. This field guide focuses on the upper Grande Ronde Basalt, Wanapum Basalt, and sediments of the Latah Formation.
Late Grande Ronde flows terminated midway into the basin to begin the formation of a topographic high that now separates a thick sediment wedge of the Vantage Member to the east of the high from a thin layer to the west. Disrupted by lava flows, streams were pushed from a west-flowing direction to a north-northwest orientation and drained the basin through a gap between steptoes toward Palouse, Washington. Emplacement of the Roza flow of the Wanapum Basalt against the western side of the topographic high was instrumental in this process, plugging west-flowing drainages and increasing deposition of Vantage sediments east of the high. The overlying basalt of Lolo covered both the Roza flow and Vantage sediments, blocking all drainages, and was in turn covered by sediments interlayered with local Saddle Mountains Basalt flows. Reestablishment of west-flowing drainages has been slow.
The uppermost Grande Ronde, the Vantage, and the Wanapum contain what is known as the upper aquifer. The water supply is controlled, in part, by thickness, composition, and distribution of the Vantage sediments. A buried channel of the Vantage likely connects the upper aquifer to Palouse, Washington, outside the basin. This field guide locates outcrops; relates them to stratigraphic well data; outlines paleogeographic basin evolution from late Grande Ronde to the present time; and notes structures, basin margin differences, and features that influence upper aquifer water supply.
Metamorphic history of the Belt Supergroup and underlying Paleoproterozoic basement rocks in the western part of the Clearwater complex, Idaho
Authors: Baldwin Julia A. , Lewis Reed S. , Vervoort Jeffrey D. , McDonie Clay D. 
Published: May 2016

Abstract
This field guide covers the Precambrian geology of the western portion of the Clearwater complex and surrounding area in north-central Idaho in the vicinity of Marble Creek within the St. Joe National Forest. The regional geology of the Marble Creek area includes Precambrian basement orthogneisses, possible basement metasupracrustal rocks, and overlying metamorphosed Belt Supergroup strata. These rocks are exhumed within the western portion of the Cretaceous-Eocene Clearwater metamorphic core complex. This guide focuses on the western part of the Clearwater complex in the vicinity of Marble Creek. Outcrops of Paleoproterozoic basement and overlying Mesoproterozoic metasedimentary units provide a better understanding of the Precambrian magmatic and metamorphic history of this region. The road log in this guide describes the regional geology in a south to north transect from Clarkia, Idaho, to the confluence of Marble Creek with the St. Joe River.
Use of novel psychoactive substances by inpatients on general adult psychiatric wards
Authors: Stanley Jack L , Mogford Daniel V , Lawrence Rebecca J , Lawrie Stephen M 
Published: May 2016

ObjectivesNon-illicit alternatives to controlled drugs, known as novel psychoactive substances (NPS), have recently risen to prominence. They are readily available, with uncertain pharmacology and no widely available assay. Given that psychiatric patients are at risk of comorbid substance abuse, we hypothesised that NPS use would be present in the psychiatric population, and sought to determine its prevalence and investigate the characteristics of those who use these drugs with a retrospective review of discharge letters.SettingGeneral adult inpatient wards of a psychiatric hospital in a Scottish city.ParticipantsAll adult inpatients (18–65) discharged from general psychiatric wards between 1 July 2014 and 31 December 2014. Of the 483 admissions identified, 46 were admissions for maintenance electroconvulsive therapy (ECT) and were excluded. Of the remaining 437 admissions, 49 discharge letters were unobtainable, leaving 388 admissions to analyse.Primary outcome measureThe mention, or lack thereof, of NPS use in discharge letters was our planned primary outcome measure and was also the primary outcome measure we used in our analysis.ResultsNPS use was identified in 22.2% of admissions, contributing to psychiatric symptoms in 59.3%. In comparison to non-users, NPS users were younger (p<0.01), male and more likely to have a forensic history ((p<0.001) for both). The diagnosis of drug-induced psychosis was significantly more likely in NPS users (p<0.001, OR 18.7, 95% CI 8.1 to 43.0) and the diagnosis of depression was significantly less likely (p<0.005, OR 0.133, CI 0.031 to 0.558). Use of cannabis was significantly more likely in NPS users (p<0.001, OR 4.2, CI 2.5 to 7.1), as was substitute opiate prescribing (p<0.001, OR 3.7, CI 1.8 to 7.4).ConclusionsNPS use was prevalent among young, male psychiatric inpatients, in particular those with drug-induced psychosis and often occurred alongside illicit drug use.
Association of BMI and interpregnancy BMI change with birth outcomes in an Australian obstetric population: a retrospective cohort study
Authors: Knight-Agarwal Catherine R , Williams Lauren T , Davis Deborah , Davey Rachel , Cochrane Tom , Zhang Huanhua , Rickwood Peter 
Published: May 2016

ObjectiveTo assess maternal and neonatal outcomes associated with increasing body mass index (BMI) and interpregnancy BMI changes in an Australian obstetric population.MethodsA retrospective cohort study from 2008 to 2013 was undertaken. BMI for 14 875 women was categorised as follows: underweight (≤18 kg/m2); normal weight (19–24 kg/m2); overweight (25–29 kg/m2); obese class I (30–34 kg/m2); obese class II (35–39 kg/m2) and obese class III (40+ kg/m2). BMI categories and maternal, neonatal and birthing outcomes were examined using logistic regression. Interpregnancy change in BMI and the risk of adverse outcomes in the subsequent pregnancy were also examined.ResultsWithin this cohort, 751 (5.1%) women were underweight, 7431 (50.0%) had normal BMI, 3748 (25.1%) were overweight, 1598 (10.8%) were obese class I, 737 (5.0%) were obese class II and 592 (4.0%) were obese class III. In bivariate adjusted models, obese women were at an increased risk of caesarean section, gestational diabetes, hypertensive disorders of pregnancy and neonatal morbidities including macrosomia, large for gestational age (LGA), hypoglycaemia, low 5 min Apgar score and respiratory distress. Multiparous women who experienced an interpregnancy increase of ≥3 BMI units had a higher adjusted OR (AOR) (CI) of the following adverse outcomes in their subsequent pregnancy: low 5-min Apgar score 3.242 (1.557 to 7.118); gestational diabetes mellitus (GDM) 3.258 (1.129 to 10.665) and hypertensive disorders of pregnancy 3.922 (1.243 to 14.760). These women were more likely to give birth vaginally 2.030 (1.417 to 2.913). Conversely, women whose parity changed from 0 to 1 and who experienced an interpregnancy increase of ≥3 BMI units had a higher AOR (CI) of caesarean section in their second pregnancy 1.806 (1.139 to 2.862).ConclusionsWomen who are overweight or obese have a significantly increased risk of various adverse outcomes. Interpregnancy weight gain, regardless of parity and baseline BMI, also increases various adverse outcomes. Effective weight management strategies are needed.
Health coaching and pedometers to enhance physical activity and prevent falls in community-dwelling people aged 60 years and over: study protocol for the Coaching for Healthy AGEing (CHAnGE) cluster randomised controlled trial
Authors: Tiedemann Anne , Rissel Chris , Howard Kirsten , Tong Allison , Merom Dafna , Smith Stuart , Wickham James , Bauman Adrian , Lord Stephen R , Vogler Constance , Lindley Richard I , Simpson Judy M , Allman-Farinelli Margaret , Sherrington Catherine 
Published: May 2016

IntroductionPrevention of falls and promotion of physical activity are essential for maximising well-being in older age. However, there is evidence that promoting physical activity among older people without providing fall prevention advice may increase fall rates. This trial aims to establish the impact of a physical activity and fall prevention programme compared with a healthy eating programme on physical activity and falls among people aged 60+ years.Methods and analysisThis cluster randomised controlled trial will involve 60 groups of community-dwelling people aged 60+ years. Participating groups will be randomised to: (1) a physical activity and fall prevention intervention (30 groups), involving written information, fall risk assessment and prevention advice, a pedometer-based physical activity tracker and telephone-based health coaching; or (2) a healthy eating intervention (30 groups) involving written information and telephone-based dietary coaching. Primary outcomes will be objectively measured physical activity at 12 months post-randomisation and self-reported falls throughout the 12-month trial period. Secondary outcomes include: the proportion of fallers, the proportion of people meeting the Australian physical activity guidelines, body mass index, eating habits, mobility goal attainment, mobility-related confidence, quality of life, fear of falling, risk-taking behaviour, mood, well-being, self-reported physical activity, disability, and health and community service use. The between-group difference in the number of falls per person-year will be analysed using negative binomial regression models. For the continuously scored primary and secondary outcome measures, linear regression adjusted for corresponding baseline scores will assess the effect of group allocation. Analyses will be preplanned, conducted while masked to group allocation, will take into account cluster randomisation, and will use an intention-to-treat approach.Ethics and disseminationProtocol has been approved by the Human Research Ethics Committee at The University of Sydney, Australia (number 2015/517). Results will be disseminated via peer-reviewed journal articles, international conference presentations and participants' newsletters.Trial registration numberACTRN12615001190594.
Right Ventricular Function in Peripartum Cardiomyopathy at Presentation Is Associated With Subsequent Left Ventricular Recovery and Clinical Outcomes
Authors: Blauwet Lori A. , Delgado-Montero Antonia , Ryo Keiko , Marek Josef J. , Alharethi Rami , Mather Paul J. , Modi Kalgi , Sheppard Richard , Thohan Vinay , Pisarcik Jessica , McNamara Dennis M. , Gorcsan John 
Published: May 2016

Background—
Peripartum cardiomyopathy has variable disease progression and left ventricular (LV) recovery. We hypothesized that baseline right ventricular (RV) size and function are associated with LV recovery and outcome.

Methods and Results—
Investigations of Pregnancy-Associated Cardiomyopathy was a prospective 30-center study of 100 peripartum cardiomyopathy women with LV ejection fraction (LVEF) <45% within 13 weeks after delivery. Baseline RV function was assessed by echocardiographic end-diastolic area, end-systolic area, fractional area change, tricuspid annular plane excursion, and RV speckle-tracking longitudinal strain. LV recovery was defined as LVEF of ≥50% at 1 year, persistent severe LV dysfunction as LVEF of ≤35%, and major events as death, transplant, or LV assist device implantation. RV measurements were feasible for 90 of the 96 patients (94%) with echocardiograms available. Mean baseline LVEF was 36±9%. RV fractional area change was <35% in 38% of patients. Of 84 patients with 1-year follow-up data, 63 (75%) had LV recovery and 11 (13%) had LVEF of ≤35% or a major event (4 LV assist devices and 2 deaths). Tricuspid annular plane excursion and RV strain did not predict outcome. Baseline RV fractional area change by multivariable analysis was independently associated with subsequent LV recovery and clinical outcome.

Conclusions—
Peripartum cardiomyopathy patients had a high incidence of LV recovery, but a significant minority had persistent LV dysfunction or a major clinical event by 1 year. RV function per echocardiographic fractional area change at presentation was associated with subsequent LV recovery and clinical outcomes and thus is prognostically important.
Enhancing Working Memory Training with Transcranial Direct Current Stimulation
Authors: Au Jacky , Katz Benjamin , Buschkuehl Martin , Bunarjo Kimberly , Senger Thea , Zabel Chelsea , Jaeggi Susanne M. , Jonides John 
Published: May 2016

Abstract
Working memory (WM) is a fundamental cognitive ability that supports complex thought but is limited in capacity. Thus, WM training interventions have become very popular as a means of potentially improving WM-related skills. Another promising intervention that has gained increasing traction in recent years is transcranial direct current stimulation (tDCS), a noninvasive form of brain stimulation that can modulate cortical excitability and temporarily increase brain plasticity. As such, it has the potential to boost learning and enhance performance on cognitive tasks. This study assessed the efficacy of tDCS to supplement WM training. Sixty-two participants were randomized to receive either right prefrontal, left prefrontal, or sham stimulation with concurrent visuospatial WM training over the course of seven training sessions. Results showed that tDCS enhanced training performance, which was strikingly preserved several months after training completion. Furthermore, we observed stronger effects when tDCS was spaced over a weekend break relative to consecutive daily training, and we also demonstrated selective transfer in the right prefrontal group to nontrained tasks of visual and spatial WM. These findings shed light on how tDCS may be leveraged as a tool to enhance performance on WM-intensive learning tasks.
Development of a highly specific enzyme immunoassay for oxytocin and its use in plasma samples
Authors: Haraya Shiomi , Karasawa Koji , Sano Yoshihiro , Ozawa Kimiko , Kato Nobumasa , Arakawa Hidetoshi 
Published: May 2016

Background The peptide hormone oxytocin acts in the central nervous system and plays an important role in various complex social behaviours. We report the production of a high affinity and specificity antibody for oxytocin and its use in a highly sensitive enzyme immunoassay. Biotin that was chemically bound to oxytocin derivative containing zero to six lysines as bridge was the labelled antigen. Seven labelled antigens were used to develop a highly sensitive enzyme immunoassay. Methods Antioxytocin antiserum was obtained by immunization of oxytocin-bovine thyrogloblin conjugate to rabbit. Oxytocin sample was added to the second antibody-coated microtitre plate and allowed to react overnight at 4℃, then biotinylated oxytocin was added 1 h at 4℃, and horseradish peroxidase-labelled avidin was added and incubated for 1 h at room temperature. The plate was then washed. Horseradish peroxidase activity was measured by a colorimetric method using o-phenylenediamine (490 nm). Results The sensitivity of the enzyme immunoassay improved as the number of lysine residues increased; consequently, biotinylated oxytocin bridged with five lysines was used. A standard curve for oxytocin ranged from 1.0 to 1000 pg/assay. The detection limit of the assay was 2.36 pg, and the reproducibility was 3.6% as CV% ( n = 6). Cross-reactivity with vasopressin and vasotocin was less than 0.01%. Conclusion The sensitivity of the enzyme immunoassay could be improved by increasing the number of lysine residues on the biotin-labelled antigen. The proposed method is sensitive and more specific than conventional immunoassays for oxytocin and can be used to determine plasma oxytocin concentrations.
National turnaround time survey: professional consensus standards for optimal performance and thresholds considered to compromise efficient and effective clinical management
Authors: McKillop Derek J , Auld Peter 
Published: May 2016

Background Turnaround time can be defined as the time from receipt of a sample by the laboratory to the validation of the result. The Royal College of Pathologists recommends that a number of performance indicators for turnaround time should be agreed with stakeholders. The difficulty is in arriving at a goal which has some evidence base to support it other than what may simply be currently achievable technically. This survey sought to establish a professional consensus on the goals and meaning of targets for laboratory turnaround time. Methods A questionnaire was circulated by the National Audit Committee to 173 lead consultants for biochemistry in the UK. The survey asked each participant to state their current target turnaround time for core investigations in a broad group of clinical settings. Each participant was also asked to provide a professional opinion on what turnaround time would pose an unacceptable risk to patient safety for each departmental category. A super majority (2/3) was selected as the threshold for consensus. Results The overall response rate was 58% ( n = 100) with a range of 49–72% across the individual Association for Clinical Biochemistry and Laboratory Medicine regions. The consensus optimal turnaround time for the emergency department was <1 h with >2 h considered unacceptable. The times for general practice and outpatient department were <24 h and >48 h and for Wards <4 h and >12 h, respectively. Conclusions We consider that the figures provide a useful benchmark of current opinion, but clearly more empirical standards will have to develop alongside other aspects of healthcare delivery.
Effective use of hybrid turbine ventilator to improve thermal performance in Malaysian tropical houses
Authors: Al-Obaidi Karam M , Ismail Muhammad Azzam , Abdul Rahman Abdul Malek 
Published: May 2016

An efficient design approach that involves extracting heat build-up in tropical buildings is a means to reduce total dependency on air-conditioning systems. The hybrid turbine ventilator can be regarded as a development technique to address this issue. However, identifying its efficient application in buildings remains questionable. This study aims to develop the research on the hybrid turbine ventilator for the establishment of energy-efficient designs in houses by evaluating its effective use in specific space volume based on several empirical comparisons. The performance of passive and hybrid turbine ventilators in attic space, room, and (attic space + room) with opened and closed windows was compared. The study was conducted in an actual building model, and data were collected onsite with a data acquisition system. The objective was achieved by investigation, and the findings can be used to identify the levels of heat build-up in each strategy. Examination of thermal performance confirmed the appropriate use of the hybrid turbine ventilator in Malaysian landed houses. Practical application:This research contributes directly to energy efficient design in tropical buildings. The outcomes inform a better decision on the effective use of hybrid turbine ventilator in low-rise buildings. The study provides ample evidence on the great potential of using hybrid turbine ventilator by developing its investigation in tropical architecture. The study will assist engineers and architects to evaluate hybrid turbine ventilator performance effectively.