bluuebunny/crossref_metadata_2025_split huggingface dataset:
An HRE-Binding Py-Im Polyamide Impairs Hypoxic Signaling in Tumors
Authors: Szablowski Jerzy O. , Raskatov Jevgenij A. , Dervan Peter B. 
Published: December 2015

Abstract
Hypoxic gene expression contributes to the pathogenesis of many diseases, including organ fibrosis, age-related macular degeneration, and cancer. Hypoxia-inducible factor-1 (HIF1), a transcription factor central to the hypoxic gene expression, mediates multiple processes including neovascularization, cancer metastasis, and cell survival. Pyrrole-imidazole polyamide 1 has been shown to inhibit HIF1-mediated gene expression in cell culture but its activity in vivo was unknown. This study reports activity of polyamide 1 in subcutaneous tumors capable of mounting a hypoxic response and showing neovascularization. We show that 1 distributes into subcutaneous tumor xenografts and normal tissues, reduces the expression of proangiogenic and prometastatic factors, inhibits the formation of new tumor blood vessels, and suppresses tumor growth. Tumors treated with 1 show no increase in HIF1α and have reduced ability to adapt to the hypoxic conditions, as evidenced by increased apoptosis in HIF1α-positive regions and the increased proximity of necrotic regions to vasculature. Overall, these results show that a molecule designed to block the transcriptional activity of HIF1 has potent antitumor activity in vivo, consistent with partial inhibition of the tumor hypoxic response. Mol Cancer Ther; 15(4); 608–17. ©2015 AACR.
Alai! Alai! – a new species of the Gloydius halys (Pallas, 1776) complex (Viperidae, Crotalinae), including a brief review of the complex
Authors: Wagner Philipp , Tiutenko Arthur , Mazepa Glib , Borkin Leo J. , Simonov Evgeniy 
Published: December 2015

During a scientific field expedition to the Alai-Pamir range five specimens of the genus Gloydius have been collected in the larger Alai. A morphological and genetical examination of the specimens has shown that they are part of the G. halys complex, but represent a new taxon which is characterized by the following unique character combination: It is a slender and moderately stout small snake, up to 479 mm total length. The head has nine symmetrical plates on the upper head, 7 supralabial and 8-9 infralabial scales. Body scales in 20-22 rows around midbody, 143-156 ventral and 35-45 usually paired subcaudal scales. The cloacal plate not divided. The general coloration consists of various different tones of olive, tan and brown, having a distinct head, but an indistinct body pattern with, excluding the tail, 26-29 transverse crossbands, which are not extending to the sides of the body. The haplotype network shows the new species within the G. halys complex and close related to both, G. h. halys and G. h. caraganus. So far the new described species is only known from the Alai range. However, various Gloydius specimens are found in Kyrgyzstan and because of the complicated taxonomy those specimens have to re-identified to clarify their status and the status of the new species.
The South American Water Balance: The Influence of Low-Level Jets
Authors: Guedes do Nascimento Marília , Herdies Dirceu Luis , Oliveira de Souza Diego 
Published: December 2015

Abstract
To study the climatology of the water balance over South America and analyze the influence of low-level jets (LLJs), a climate study of the water balance and its main components was performed, specifically in the Amazon and La Plata basin (LPB) region, from 1979 to 2008. The results showed that on average for the analysis period, the Amazon basin and LPB performed as a sink of moisture (ET &lt; P) and as a moisture convergence for the regions, which accounted for approximately 62% and 43% of the precipitation, respectively. During the study period, 884 days with an occurrence of LLJs were observed, occurring most frequently during the winter and around 0000 and 0600 UTC. When considering the water balance for the days with LLJs, it was observed that the Amazon acts as a source of moisture, especially in the dry season, and that the LPB behaves as a sink during all months. The influence of the LLJ as a modulator for precipitation on the LPB is clear, as the precipitation is 32% higher during the LLJ events compared with days without LLJs. This main pattern shows that the moisture convergence trough of the LLJs is crucial for the water balance on the LPB, whereas evapotranspiration is a more important variable of the water balance on the Amazon basin with or without the LLJs.
Diversity, Nonlinearity, Seasonality, and Memory Effect in ENSO Simulation and Prediction Using Empirical Model Reduction
Authors: Chen Chen , Cane Mark A. , Henderson Naomi , Lee Dong Eun , Chapman David , Kondrashov Dmitri , Chekroun Mickaël D. 
Published: December 2015

Abstract
A suite of empirical model experiments under the empirical model reduction framework are conducted to advance the understanding of ENSO diversity, nonlinearity, seasonality, and the memory effect in the simulation and prediction of tropical Pacific sea surface temperature (SST) anomalies. The model training and evaluation are carried out using 4000-yr preindustrial control simulation data from the coupled model GFDL CM2.1. The results show that multivariate models with tropical Pacific subsurface information and multilevel models with SST history information both improve the prediction skill dramatically. These two types of models represent the ENSO memory effect based on either the recharge oscillator or the time-delayed oscillator viewpoint. Multilevel SST models are a bit more efficient, requiring fewer model coefficients. Nonlinearity is found necessary to reproduce the ENSO diversity feature for extreme events. The nonlinear models reconstruct the skewed probability density function of SST anomalies and improve the prediction of the skewed amplitude, though the role of nonlinearity may be slightly overestimated given the strong nonlinear ENSO in GFDL CM2.1. The models with periodic terms reproduce the SST seasonal phase locking but do not improve the prediction appreciably. The models with multiple ingredients capture several ENSO characteristics simultaneously and exhibit overall better prediction skill for more diverse target patterns. In particular, they alleviate the spring/autumn prediction barrier and reduce the tendency for predicted values to lag the target month value.
A Temporal Kernel Method to Compute Effective Radiative Forcing in CMIP5 Transient Simulations*
Authors: Larson Erik J. L. , Portmann Robert W. 
Published: December 2015

Abstract
Effective radiative forcing (ERF) is calculated as the flux change at the top of the atmosphere after allowing rapid adjustments resulting from a forcing agent, such as greenhouse gases. Rapid adjustments include changes to atmospheric temperature, water vapor, and clouds. Accurate estimates of ERF are necessary in order to understand the drivers of climate change. This work presents a new method of calculating ERF using a kernel derived from the time series of a model variable (e.g., global mean surface temperature) in a model-step change experiment. The top-of-atmosphere (TOA) radiative imbalance has the best noise tolerance for retrieving the ERF of the model variables tested. This temporal kernel method is compared with an energy balance method, which equates ERF to the TOA radiative imbalance plus the scaled surface temperature change. Sensitivities and biases of these methods are quantified using output from phase 5 of the the Coupled Model Intercomparison Project (CMIP5). The temporal kernel method is likely more accurate for models in which a linear fit is a poor approximation for the relationship between temperature change and TOA imbalance. The difference between these methods is most apparent in forcing estimates for the representative concentration pathway 8.5 (RCP8.5) scenario. The CMIP5 multimodel mean ERF calculated for large volcanic eruptions is 80% of the adjusted forcing reported by the IPCC Fifth Assessment Report (AR5). This suggests that about 5% more energy has come into the earth system since 1870 than suggested by the IPCC AR5.
A Climatology of Southern Appalachian Cold-Air Damming*
Authors: Rackley Jared A. , Knox John A. 
Published: December 2015

Abstract
A 30-yr climatology (1981–2010) of cold-air damming (CAD) events in the southern Appalachians was conducted using hourly surface observations and North American Regional Reanalysis (NARR) data. Analysis of the spatial distribution and frequency of these events reveals that some part of the Southeast is affected by CAD on 50 days out of each year, and even the northern Florida panhandle and much of Alabama experience CAD conditions on about 30 days annually. Spatially, different CAD types tend to exhibit one of two patterns in the southernmost extent of the cold-air dome: a more southerly dome with a ridge axis oriented from north-northeast to south-southwest or a more westerly dome with a ridge axis in a northeast to west-southwest orientation. These patterns may be the result of both splitting around the region of higher terrain in east-central Alabama and Coriolis forcing in stronger CAD types with higher wind speeds. Analysis of the frequency of CAD by type on a month-by-month and year-by-year basis confirms previous work that CAD is much more frequent during the cold season versus the warm season, with CAD occurring on 6.8 days month−1 during December and only 1.3 days month−1 during July. Analysis was also stratified by CAD type, revealing that weak/dry events were the most common. Classical type events with stronger and more favorably positioned parent highs exhibited the longest average duration, nearly 45 h, while other CAD types averaged approximately half as long.
Analysis of Food Hub Commerce and Participation Using Agent-Based Modeling
Authors: Krejci Caroline C. , Stone Richard T. , Dorneich Michael C. , Gilbert Stephen B. 
Published: December 2015

Objective: Factors influencing long-term viability of an intermediated regional food supply network (food hub) were modeled using agent-based modeling techniques informed by interview data gathered from food hub participants. Background: Previous analyses of food hub dynamics focused primarily on financial drivers rather than social factors and have not used mathematical models. Method: Based on qualitative and quantitative data gathered from 22 customers and 11 vendors at a midwestern food hub, an agent-based model (ABM) was created with distinct consumer personas characterizing the range of consumer priorities. A comparison study determined if the ABM behaved differently than a model based on traditional economic assumptions. Further simulation studies assessed the effect of changes in parameters, such as producer reliability and the consumer profiles, on long-term food hub sustainability. Results: The persona-based ABM model produced different and more resilient results than the more traditional way of modeling consumers. Reduced producer reliability significantly reduced trade; in some instances, a modest reduction in reliability threatened the sustainability of the system. Finally, a modest increase in price-driven consumers at the outset of the simulation quickly resulted in those consumers becoming a majority of the overall customer base. Conclusion: Results suggest that social factors, such as desire to support the community, can be more important than financial factors. Application: An ABM of food hub dynamics, based on human factors data gathered from the field, can be a useful tool for policy decisions. Similar approaches can be used for modeling customer dynamics with other sustainable organizations.
Ecuador’s Buen vivir
Authors: Caria Sara , Domínguez Rafael 
Published: December 2015

The concept of buen vivir (good living) has attracted interest far beyond its source in the Andean ethnic tradition. It is being debated internationally as a contribution to development theory and has become the fundamental purpose of Ecuador’s policy since the adoption of the new constitution in 2008. There are, however, deep contradictions between the constitutional prescriptions and spirit of buen vivir and recently formulated policies that reveal a pragmatic approach on the part of the government. These contradictions suggest that, far from being a strategic orientation for effective policy making, buen vivir serves as a new ideology and is being used to support a reform plan based on a quite traditional understanding of the concept of development. El concepto de “buen vivir” ha despertado interés más allá de su origen en la tradición étnica andina. Su contribución a la teoría del desarrollo es parte del debate internacional y también se ha convertido en el objetivo fundamental de la política pública del Ecuador desde que se adoptó la nueva constitución en 2008. Hay, sin embargo, profundas contradicciones entre las prescripciones constitucionales y el espíritu del buen vivir y algunas políticas formuladas recientemente que revelan un enfoque pragmático de parte del gobierno. Estas contradicciones sugieren que, lejos de ser una orientación estratégica para la formulación de políticas más eficaces, el buen vivir sirve como una nueva ideología y está siendo usado para apoyar un plan de reforma basado en una interpretación bastante tradicional del concepto de desarrollo.
Antecedents to Academic Performance of College Students
Authors: Duggal Minakshi , Mehta Pooja 
Published: December 2015

Academic performance of students is affected by a number of factors. The aim of this study is to investigate the antecedents to the academic performance of college students. The data analysis was done with SPSS factor analysis and chi-square test of Independence. The study reveals that there are a number of factors that influence the academic performance of students and contributes towards the same in different ways. Educational institutions can use the results for policy formulation, parents can use them as a guide for their children and students can come across some factors about which they themselves are not be aware of (hidden factors). Investigations have been carried out on the influence of factors such as gender or parent’s education. Beside these, there are also some additional factors that affect the students, but little effort has been made till date. This gap in research has motivated the need to conduct the present study.
The Clinical Implication of Blood Product Transfusion on Morbidity and Mortality of Ruptured Abdominal Aortic Aneurysm
Authors: Kordzadeh Ali , Askari Alan , Parsa Ali D. , Browne Tom , Panayiotopoulos Yiannis P. 
Published: December 2015

Background:Transfusion of blood products occurs frequently in ruptured abdominal aortic aneurysm surgery (rAAA). The aim of this study is to establish the impact of packed red blood cell (PRBC), fresh frozen plasma (FFP), and platelet (PLT) transfusion on the 30-day mortality and morbidity (thrombotic versus non thrombotic complications) of rAAAs.Methods:A retrospective study of 90 consecutive rAAAs from November 2007 to June 2015 was conducted. Multivariable regression models were produced to determine blood products associated with 30-day morbidity and mortality post-rAAA.Results:The overall mortality was 14.6%. At multivariable analyses, transfusion with FFP (>3 units) was independently associated with an increased risk of mortality (odds ratio [OR]: 11.27, 95% confidence interval [CI]: 1.13-96.72, P = .027). The overall morbidity was 26.8%, and subgroup analysis (thrombotic vs nonthrombotic complications) demonstrated transfusion of PLTs (>1 pool) to be independently associated with thrombotic events (OR: 4.3, 95% CI: 1.37-13.6, P = .012). Thrombotic events were responsible for 50% (n = 11 of 22) of all morbidities and mortalities (n = 6 of 12).Conclusion:Transfusion of FFP and PLTs may be associated with an increased risk of postoperative morbidity and mortality. The use of these blood components should be considered in the context of the patient’s clinical and laboratory data as opposed to a fixed ratio to PRBCs. This may result in the reduction in thrombotic complications emerging in rAAA cohort.
Electric fields are novel determinants of human macrophage functions
Authors: Hoare Joseph I , Rajnicek Ann M , McCaig Colin D , Barker Robert N , Wilson Heather M 
Published: December 2015

Abstract
Macrophages are key cells in inflammation and repair, and their activity requires close regulation. The characterization of cues coordinating macrophage function has focused on biologic and soluble mediators, with little known about their responses to physical stimuli, such as the electrical fields that are generated naturally in injured tissue and which accelerate wound healing. To address this gap in understanding, we tested how properties of human monocyte-derived macrophages are regulated by applied electrical fields, similar in strengths to those established naturally. With the use of live-cell video microscopy, we show that macrophage migration is directed anodally by electrical fields as low as 5 mV/mm and is electrical field strength dependent, with effects peaking ∼300 mV/mm. Monocytes, as macrophage precursors, migrate in the opposite, cathodal direction. Strikingly, we show for the first time that electrical fields significantly enhance macrophage phagocytic uptake of a variety of targets, including carboxylate beads, apoptotic neutrophils, and the nominal opportunist pathogen Candida albicans, which engage different classes of surface receptors. These electrical field-induced functional changes are accompanied by clustering of phagocytic receptors, enhanced PI3K and ERK activation, mobilization of intracellular calcium, and actin polarization. Electrical fields also modulate cytokine production selectively and can augment some effects of conventional polarizing stimuli on cytokine secretion. Taken together, electrical signals have been identified as major contributors to the coordination and regulation of important human macrophage functions, including those essential for microbial clearance and healing. Our results open up a new area of research into effects of naturally occurring and clinically applied electrical fields in conditions where macrophage activity is critical.
The Use of Somatosensory Evoked Potentials to Determine the Relationship Between Intraoperative Arterial Blood Pressure and Intraoperative Upper Extremity Position–Related Neurapraxia in the Prone Surrender Position During Spine Surgery: A Retrospective Analysis
Authors: Kamel Ihab , Zhao Huaqing , Koch Stephen A. , Brister Neil , Barnette Rodger E. 
Published: December 2015

BACKGROUND:
Peripheral nerve injury is a significant perioperative problem. Intraoperative position-related neurapraxia may indicate impending peripheral nerve injury and can be detected by changes in somatosensory evoked potentials (SSEP). The purpose of this retrospective analysis of spine surgeries performed under general anesthesia with SSEP monitoring was to determine the relationship between intraoperative mean arterial blood pressure (MAP) and intraoperative upper extremity position–related neurapraxia in the prone surrender (superman) position.

METHODS:
We reviewed a computerized database of spine surgeries performed on adult patients in the prone surrender position. The authors reviewed intraoperative SSEP monitoring reports to identify the patients who developed intraoperative upper extremity position–related neurapraxia (case group) and patients who did not (control group). Propensity matching was performed to derive 2 demographically matched groups. Preoperative and intraoperative variables were included in the univariate Cox regression analysis of risk factors associated with neurapraxia. Multivariate Cox regression models were used to identify the independent risk factors.

RESULTS:
One hundred fifty-two patients were included in the analysis. The case group included 32 patients, whereas the control group included 120 matched patients. Intraoperative MAP <55 mm Hg for a total duration of ≥5 minutes was an independent risk factor associated with a greater incidence of upper extremity position–related neurapraxia compared with a duration of <5 minutes with MAP <55 mm Hg (hazard ratio, 3.43; confidence interval, 1.445–8.148; P = 0.0052). Intraoperative MAP >80 mm Hg for a total duration of >55 minutes was an independent predictor associated with a lower incidence of neurapraxia compared with a total duration ≤55 minutes (hazard ratio, 0.341; confidence interval, 0.163–0.717; P = 0.0045).

CONCLUSIONS:
In this study, we identified the changes in intraoperative MAP as independent predictors associated with upper extremity position–related neurapraxia in the prone surrender position under general anesthesia.
Differences in infectivity between endosymbiotic Chlorella variabilis cultivated outside host Paramecium bursaria for 50 years and those immediately isolated from host cells after one year of reendosymbiosis
Authors: Kodama Y. , Fujishima M. 
Published: December 2015

ABSTRACT
Chlorella variabilis strain NC64A is an intracellular photobiont of the ciliate Paramecium bursaria. NC64A was isolated from P. bursaria nearly 50 years ago and was thereafter cultivated outside the host. This study was undertaken to detect changes in its infectivity to P. bursaria and its auxotrophy for growth outside the host induced during long-term cultivation. NC64A can grow in Modified Bold's Basal Medium but not in C medium, whereas another symbiotic Chlorella variabilis strain, 1N, that was recently isolated from the host grew in C medium but not in Modified Bold's Basal Medium. With regards infectivity, NC64A in the logarithmic phase of growth showed low infectivity to alga-removed P. bursaria cells, whereas those in the early stationary phase showed high infectivity of about 30%. Those in the decay phase of growth showed no infectivity. Results show that NC64A has infectivity, but the infection rate depends on their culture age in the growth curve. Furthermore, NC64A that had been re-infected to P. bursaria for more than one year and isolated from the host showed a nearly 100% infection rate, which indicates that NC64A can recover its infectivity by re-infection to P. bursaria.
Unique geometry of sister kinetochores in human oocytes during meiosis I may explain maternal age-associated increases in chromosomal abnormalities
Authors: Patel Jessica , Tan Seang Lin , Hartshorne Geraldine M. , McAinsh Andrew D. 
Published: December 2015

ABSTRACT
The first meiotic division in human oocytes is highly error-prone and contributes to the uniquely high incidence of aneuploidy observed in human pregnancies. A successful meiosis I (MI) division entails separation of homologous chromosome pairs and co-segregation of sister chromatids. For this to happen, sister kinetochores must form attachments to spindle kinetochore-fibres emanating from the same pole. In mouse and budding yeast, sister kinetochores remain closely associated with each other during MI, enabling them to act as a single unified structure. However, whether this arrangement also applies in human meiosis I oocytes was unclear. In this study, we perform high-resolution imaging of over 1900 kinetochores in human oocytes, to examine the geometry and architecture of the human meiotic kinetochore. We reveal that sister kinetochores in MI are not physically fused, and instead individual kinetochores within a pair are capable of forming independent attachments to spindle k-fibres. Notably, with increasing female age, the separation between kinetochores increases, suggesting a degradation of centromeric cohesion and/or changes in kinetochore architecture. Our data suggest that the differential arrangement of sister kinetochores and dual k-fibre attachments may explain the high proportion of unstable attachments that form in MI and thus indicate why human oocytes are prone to aneuploidy, particularly with increasing maternal age.