bluuebunny/crossref_metadata_2025_split huggingface dataset:
Composite activity type and stride-specific energy expenditure estimation model for thigh-worn accelerometry
Authors: Lendt Claas , Hansen Niklas , Froböse Ingo , Stewart Tom 
Published: September 2024

Abstract
Background
Accurately measuring energy expenditure during physical activity outside of the laboratory is challenging, especially on a large scale. Thigh-worn accelerometers have gained popularity due to the possibility to accurately detect physical activity types. The use of machine learning techniques for activity classification and energy expenditure prediction may improve accuracy over current methods. Here, we developed a novel composite energy expenditure estimation model by combining an activity classification model with a stride specific energy expenditure model for walking, running, and cycling.

Methods
We first trained a supervised deep learning activity classification model using pooled data from available adult accelerometer datasets. The composite energy expenditure model was then developed and validated using additional data based on a sample of 69 healthy adult participants (49% female; age = 25.2 ± 5.8 years) who completed a standardised activity protocol with indirect calorimetry as the reference measure.

Results
The activity classification model showed an overall accuracy of 99.7% across all five activity types during validation. The composite model for estimating energy expenditure achieved a mean absolute percentage error of 10.9%. For running, walking, and cycling, the composite model achieved a mean absolute percentage error of 6.6%, 7.9% and 16.1%, respectively.

Conclusions
The integration of thigh-worn accelerometers with machine learning models provides a highly accurate method for classifying physical activity types and estimating energy expenditure. Our novel composite model approach improves the accuracy of energy expenditure measurements and supports better monitoring and assessment methods in non-laboratory settings.
A grassroots approach to peer support by the Danish Reproducibility Network
Authors: Topor Marta , Bonnet Philippe , Cheplygina Veronika , Dam Vibeke Høyrup , Wildgaard Lorna 
Published: September 2024

AbstractThe Danish Reproducibility Network (DKRN) is a grassroots initiative for establishing a peer-supportive reproducibility-focused academic network in Denmark. We modelled our approach on already existing national Reproducibility Networks. We consulted with researchers and research support professionals to identify the needs of the research community. Three themes emerged around policy implementation, training and the appropriate application of reproducible practices. The network aims to address these three themes in a strategic plan, which harnesses the benefits of grassroots initiatives. The mission of the DKRN is therefore to facilitate communication, peer-support, and the exchange of ideas through a network of topic and geographical nodes. The network is open to researchers and research support professionals from all career stages and disciplines. It aligns with broader international initiatives, and national institutions, positioning itself as a contributor to the Danish research ecosystem.
Shared decision-making in end-of-life care for end-stage renal disease patients: nephrologists’ views and attitudes
Authors: Hatoum Wassiem Bassam Abu , Sperling Daniel 
Published: September 2024

Abstract
Background
The term end-stage renal disease (ESRD) refers to the final stage of chronic kidney disease. Not all ESRD patients are suitable for dialysis treatment, which despite its advantages, is not without risks. Shared nephrologist-patient decision-making could be beneficial at this stage, yet little is known about such practices in Israel. This study aimed at examining the practice of shared decision-making (SDM) between nephrologists and ESRD patients in Israel, while exploring related conflicts, ethical dilemmas, and considerations.

Methods
The descriptive-quantitative approach applied in this study included a validated questionnaire for nephrologists, based on Emanual and Emanual (1992). The survey, which was distributed via social-media platforms and snowball sampling, was completed by 169 nephrologists. Data analysis included t-tests for independent samples, f-tests for analysis of variance, and t-tests and f-tests for independence. Descriptive analysis examined attitudes towards SDM in end-of-life care for ESRD patients.

Results
The findings show that the research sample did not include nephrologists who typically act according to the paternalistic decision-making style. Rather, 53% of the respondents were found to act in line with the informative decision-making style, while 47% act according to the interpretive decision-making style. Almost 70% of all respondents reported their discussing quality-of-life with patients; 63.4% provide prognostic assessments; 61.5% inquire about the patient’s desired place of death; 58.6% ask about advance directives or power-of-attorney; and 57.4% inquire about cultural and religious beliefs in end-of-life treatment. Additionally, informative nephrologists tend to promote the patients’ autonomy over their health (P < 0.001); they are also in favor of conservative treatment, compared to paternalistic and interpretive nephrologists, and use less invasive methods than other nephrologists (P = 0.02).

Conclusions
Nephrologists in Israel only partially pursue an SDM model, which has the potential to improve quality-of-care for ESRD patients and their families. SDM programs should be developed and implemented for increasing such practices among nephrologists, thereby expanding the possibilities for providing conservative care at end-of-life.
Fine spatial scale assessment of structure and configuration of vegetation cover for northern bobwhites in grazed pastures
Authors: Avila-Sanchez J. Silverio , Perotto-Baldivieso Humberto L. , Massey Lori D. , Ortega-S J. Alfonso , Brennan Leonard A. , Hernández Fidel 
Published: September 2024

Abstract
Background
Monitoring forage in livestock operations is critical to sustainable rangeland management of soil and ecological processes that provide both livestock and wildlife habitat. Traditional ground-based sampling methods have been widely used and provide valuable information; however, they are time-consuming, labor-intensive, and limited in their ability to capture larger extents of the spatial and temporal dynamics of rangeland ecosystems. Drones provide a solution to collect data to larger extents than field-based methods and with higher-resolution than traditional remote sensing platforms. Our objectives were to (1) assess the accuracy of vegetation cover height in grasses using drones, (2) quantify the spatial distribution of vegetation cover height in grazed and non-grazed pastures during the dormant (fall–winter) and growing seasons (spring–summer), and (3) evaluate the spatial distribution of vegetation cover height as a proxy for northern bobwhite (Colinus virginianus) habitat in South Texas. We achieved this by very fine scale drone-derived imagery and using class level landscape metrics to assess vegetation cover height configuration.

Results
Estimated heights from drone imagery had a significant relationship with the field height measurements in September (r2 = 0.83; growing season) and February (r2 = 0.77; dormant season). Growing season pasture maintained residual landscape habitat configuration adequate for bobwhites throughout the fall and winter of 2022–2023 following grazing. Dormant season pasture had an increase in bare ground cover, and a shift from many large patches of tall herbaceous cover (40–120 cm) to few large patches of low herbaceous cover (5–30 cm) (p < 0.05).

Conclusions
Drones provided high-resolution imagery that allowed us to assess the spatial and temporal changes of vertical herbaceous vegetation structure in a semi-arid rangeland subject to grazing. This study shows how drone imagery can be beneficial for wildlife conservation and management by providing insights into changes in fine-scale vegetation spatial and temporal heterogeneity from livestock grazing.
An analysis of the contribution of the informal milk market to nutrition in Kenya
Authors: Revoredo-Giha Cesar , Zavala-Nacul Hasibi 
Published: September 2024

Abstract
Background
The dairy sector in Kenya is an important part of the agricultural GDP of the country. Its legal framework was reformed in 2004 to address the economic importance that the informal sector (i.e. trading on raw milk) had for smallholders’ producers. However, this reform was accused of being a pro-poor policy instead of focusing on the development of the formal dairy sector. In recent years, there has been pressure to go back to the pre-2004 regulatory system and to illegalize the raw milk trade. An aspect that has been absent from the discussion is the contribution that the informal market has to households’ nutrition. The contribution of this paper is to address this shortcoming using the most recent Kenya Integrated Household Budget Survey for 2015–16.

Results
The results indicate that the rural annual milk consumption (70.2 L) surpasses that of urban areas (68.8 L). These values are different to those found in the literature. County consumption varies depending on the availability of milk, which appears associated to the production capacity and the climate of the region. However, regardless of the region unpacked milk is the most consumed dairy item representing 72 per cent of the total countrywide; 84 per cent of the rural total and 55 per cent of the urban area.

Conclusions
The results indicate that the sector provides significant nutrition especially to poor economic groups. Therefore, attempting to ban the informal sector would have negative consequences for Kenya's food security, impacting mostly on the nutritional security of low-income households. In addition, the nutritional aspects that the informal dairy sector provides need to be considered alongside the economic ones in future debates regarding regulatory reforms of the sector.
Adult attachment and love psychological stress among college students: the mediating role of core self-evaluation and the moderating role of meaning in life
Authors: Song Minghua , Hu Xianman , Liu Shen 
Published: September 2024

Abstract
Background
With college students going into dating relationships, dating partners become their new attachment figures. We aimed to investigate the relationship between adult attachment, and love psychological stress among college students, which also explored the roles of core self-evaluation and meaning in life.

Methods
We conducted a questionnaire survey on 813 college students using the adult attachment scale, core self-evaluation scale, meaning in life scale, and love psychological stress scale. We constructed a moderated mediation model to analyze the relationship between adult attachment and love psychological stress, as well as the mediating effect of core self-evaluation and the moderating effect of meaning in life.

Results
The results showed that after controlling for single parent or not, adult attachment significantly negatively predicted love psychological stress of college students. Core self-evaluation partially mediated the relationship between adult attachment and love psychological stress of college students. The second half of the mediation model was moderated by meaning in life, that is, with the increase of meaning in life, the negative predictive effect of core self-evaluation on love psychological stress of college students gradually strengthened. The findings of this study demonstrate the detrimental impact of adult attachment on love psychological stress of college students, as well as the mediating core self-evaluation and the moderating role of meaning in life.

Conclusions
The mediating and moderating effect of adult attachment between love psychological stress, as well as the mediating effect of core self-evaluation and the moderating effect of meaning in life were confirmed. Overall, promoting the healthy development of adult attachment and helping them shape a positive meaning in life can enhance individuals’ core self-evaluation, thus alleviating love psychological stress among college students. It can also provide references for mental health education in colleges and universities.
Characterization of indigenous lactobacilli from dairy fermented foods of Haryana as potential probiotics utilizing multiple attribute decision-making approach
Authors: Samtiya Mrinal , Bhushan Bharat , Sari T. P. , Badgujar Prarabdh C. , Chandratre Gauri A. , Singh Phool , Kumar Ashwani , Dhewa Tejpal 
Published: September 2024

AbstractThe interest in region-specific ethnic fermented foods and their functional microbiota is rising. The demands for functional foods are continuously rising, so research is going on to develop nutritious food with many beneficial attributes and low safety concerns. The present study was designed to isolate and characterize lactobacilli probiotic candidates from locally resourced fermented foods (dahi, lassi, and raabadi) to make ready-to-eat fermented functional products later. Cultures were isolated from 82 fermented food samples collected from different villages. The initial experiments of gram staining, catalase test, and carbohydrate fermentation were assessed for the morphology, purity, and primary characterization on the genus level, which was verified through molecular characterization using PCR. Seven lactobacilli strains (no. MS001-MS007) were then assessed for safety, probiotic candidacy, phytase degradation, and biofilm forming abilities. All seven bacterial cultures showed no hemolytic activity and antibiotic sensitivity against more than 14 antibiotics out of 20. All seven lactobacilli isolates were able to tolerate pH 3.0, 0.3% bile 0.5% pancreatin, lysozyme (100 mg/L to 300 mg/L) and also shown possessed phytase degradation ability. All the cultures showed antioxidative potential and biofilm formation ability. Culture MS007 showed considerably higher bile salt hydrolase activity among all the isolates, whereas MS005 possessed excellent phytate degradation ability among others. Bacterial strains were identified using 16S rRNA gene sequencing. Moreover, the order of preference of isolates was calculated using the multidimensional Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) based on probiotic and other functional properties. The most promising attributes showing cultures were recognised as Limosilactobacillus fermentum MS005 and Lactiplantibacillus plantarum MS007, which could be further used for functional food product development.

Graphical Abstract
Assessment of hepcidin in Egyptian patients with rheumatoid arthritis and its relation to anemia: a single-center study
Authors: Genedy Manal M. , Shabana Adel Abd Elsalam Hussien , Elghzaly Ashraf Antar Mohamad , Bassiouni Sherine Abdel Rahman Abdel Karim 
Published: September 2024

Abstract
Background
Rheumatoid arthritis (RA) is a chronic systemic autoimmune inflammatory disorder characterized by synovial inflammation that leads to joint damage, bony erosions, and related deformities. Between 30 and 70% of RA patients will experience anemia. Early detection of anemia is of great importance. This study aimed to evaluate the serum level of hepcidin (HEP) in RA patients and to assess its relation to disease activity and anemia. The current cross-sectional study included 44 cases with RA in addition to 44 healthy controls. The disease activity in the RA patient was assessed by using the disease activity score (DAS) 28 score-CRP. The serum levels of HEP and ferritin were assessed in both groups using enzyme-linked immunosorbent assay (ELISA) technique.

Results
Hepcidin level in the RA group was statistically significantly higher as compared to the control group (p = 0.001). The prevalence of Anemia of chronic disease (ACD) was 40.9%, and iron deficiency anemia (IDA) was 27.3% which accounted for 68.2% of the total anemia cases. The HEP level was statistically significantly higher in the RA patients with ACD than those without anemia (P = 0.028), RA patients with IDA (P < 0.001), and control group (P < 0.001). There was a statistically significant positive correlation between HEP level and serum ferritin level (p = 0.005). HEP level was significantly and inversely correlated with hemoglobin (Hb) in patients with ACD. Serum HEP level is higher in RA patients with high disease activity than those with moderate activity, low activity, and patients in remission (p = 0.380). However, the difference was not statistically significant. The best cutoff point of HEP level to identify RA patients from healthy controls was > 355.5 Pg/ml. This point showed moderate sensitivity (70.5%) with moderate specificity (63.6%) with a statistically significant value.

Conclusions
We found the anemia, and particularly ACD, is more common in RA patients. In RA patients with ACD, serum HEP levels were considerably higher. Although serum HEP showed no diagnostic significance when it came to evaluating disease activity, it could be a dependable non-invasive biomarker for the diagnosis of various forms of anemia in RA patients.
Relationship Between Radiation Dose and Markers of Insulin Resistance and Inflammation in Atomic Bomb Survivors
Authors: Tatsukawa Yoshimi , Sposto Richard , Yamada Michiko , Ohishi Waka , Imaizumi Misa , Hida Ayumi , Sakata Ritsu , Fujiwara Saeko , Nakanishi Shuhei , Ohno Haruya 
Published: September 2024

Abstract

Context
In recent studies of childhood cancer survivors, diabetes has been considered a late effect associated with high therapeutic doses of radiation therapy. Our recent study of atomic bomb (A-bomb) survivors also suggested an association between radiation dose and diabetes incidence, with exposure city and age at exposure as radiation dose effect modifiers. Insulin resistance mediated by systemic inflammation and abnormal body composition has been suggested as a possible primary mechanism for the incidence of diabetes after total body irradiation; however, no studies have examined low to moderate radiation exposure (&lt;4 Gy) and insulin resistance in A-bomb survivors.

Objective
To examine the association between radiation dose and markers of inflammation and insulin resistance.

Methods
This study investigated 3152 survivors who underwent a health examination between 2008 and 2012 and who were younger than 15 years at exposure. Multivariate linear regression analyses were used to evaluate the radiation effects on levels of markers of inflammation and insulin resistance.

Results
Radiation dose was significantly and positively associated with levels of C-reactive protein, triglycerides, homeostasis model assessment of β-cell function (HOMA-β), and HOMA of insulin resistance (HOMA-IR) after adjustment for relevant covariates including sex, city, and age at exposure. Adiponectin and high-density lipoprotein cholesterol levels were also associated significantly and negatively with radiation dose. However, city was not a dose modifier of the radiation response on these markers of inflammation and insulin resistance.

Conclusion
Insulin resistance might be a possible factor in radiation-related diabetes incidence in A-bomb survivors.
Identification of Environmental Compounds That May Trigger Early Female Puberty by Activating Human GnRHR and KISS1R
Authors: Yang Shu , Zhang Li , Khan Kamal , Travers Jameson , Huang Ruili , Jovanovic Vukasin M , Veeramachaneni Rithvik , Sakamuru Srilatha , Tristan Carlos A , Davis Erica E , Klumpp-Thomas Carleen , Witt Kristine L , Simeonov Anton , Shaw Natalie D , Xia Menghang 
Published: September 2024

Abstract
There has been an alarming trend toward earlier puberty in girls, suggesting the influence of an environmental factor(s). As the reactivation of the reproductive axis during puberty is thought to be mediated by the hypothalamic neuropeptides kisspeptin and gonadotropin-releasing hormone (GnRH), we asked whether an environmental compound might activate the kisspeptin (KISS1R) or GnRH receptor (GnRHR). We used GnRHR or KISS1R-expressing HEK293 cells to screen the Tox21 10K compound library, a compendium of pharmaceuticals and environmental compounds, for GnRHR and KISS1R activation. Agonists were identified using Ca2+ flux and phosphorylated extracellularly regulated kinase (p-ERK) detection assays. Follow-up studies included measurement of genes known to be upregulated upon receptor activation using relevant murine or human cell lines and molecular docking simulation. Musk ambrette was identified as a KISS1R agonist, and treatment with musk ambrette led to increased expression of Gnrh1 in murine and human hypothalamic cells and expansion of GnRH neuronal area in developing zebrafish larvae. Molecular docking demonstrated that musk ambrette interacts with the His309, Gln122, and Gln123 residues of the KISS1R. A group of cholinergic agonists with structures similar to methacholine was identified as GnRHR agonists. When applied to murine gonadotrope cells, these agonists upregulated Fos, Jun, and/or Egr1. Molecular docking revealed a potential interaction between GnRHR and 5 agonists, with Asn305 constituting the most conservative GnRHR binding site. In summary, using a Tox21 10K compound library screen combined with cellular, molecular, and structural biology techniques, we have identified novel environmental agents that may activate the human KISS1R or GnRHR.
Presynaptic Nrxn3 is essential for ribbon-synapse maturation in hair cells
Authors: Jukic Alma , Lei Zhengchang , Cebul Elizabeth R. , Pinter Katherine , Tadesse Yommi , Jarysta Amandine , David Sandeep , Mosqueda Natalie , Tarchini Basile , Kindt Katie 
Published: September 2024

ABSTRACT
Hair cells of the inner ear and lateral-line system rely on specialized ribbon synapses to transmit sensory information to the central nervous system. The molecules required to assemble these synapses are not fully understood. We show that Nrxn3, a presynaptic adhesion molecule, is crucial for ribbon-synapse maturation in hair cells. In both mouse and zebrafish models, the loss of Nrxn3 results in significantly fewer intact ribbon synapses. We show in zebrafish that, initially, Nrxn3 loss does not alter pre- and postsynapse numbers but, later, synapses fail to pair, leading to postsynapse loss. We also demonstrate that Nrxn3 subtly influences synapse selectivity in zebrafish lateral-line hair cells that detect anterior flow. Loss of Nrxn3 leads to a 60% loss of synapses in zebrafish, which dramatically reduces pre- and postsynaptic responses. Despite fewer synapses, auditory responses in zebrafish and mice are unaffected. This work demonstrates that Nrxn3 is a crucial and conserved molecule required for the maturation of ribbon synapses. Understanding how ribbon synapses mature is essential to generating new therapies to treat synaptopathies linked to auditory or vestibular dysfunction.