bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonvolatile nuclear spin memory enables sensor-unlimited nanoscale spectroscopy of small spin clusters
Authors: Pfender Matthias , Aslam Nabeel , Sumiya Hitoshi , Onoda Shinobu , Neumann Philipp , Isoya Junichi , Meriles Carlos A. , Wrachtrup Jörg 
Published: October 2017

AbstractIn nanoscale metrology, dissipation of the sensor limits its performance. Strong dissipation has a negative impact on sensitivity, and sensor–target interaction even causes relaxation or dephasing of the latter. The weak dissipation of nitrogen-vacancy (NV) sensors in room temperature diamond enables detection of individual target nuclear spins, yet limits the spectral resolution of nuclear magnetic resonance (NMR) spectroscopy to several hundred Hertz, which typically prevents molecular recognition. Here, we use the NV intrinsic nuclear spin as a nonvolatile classical memory to store NMR information, while suppressing sensor back-action on the target using controlled decoupling of sensor, memory, and target. We demonstrate memory lifetimes up to 4 min and apply measurement and decoupling protocols, which exploit such memories efficiently. Our universal NV-based sensor device records single-spin NMR spectra with 13 Hz resolution at room temperature.
Coherent optomechanical state transfer between disparate mechanical resonators
Authors: Weaver Matthew J. , Buters Frank , Luna Fernando , Eerkens Hedwig , Heeck Kier , de Man Sven , Bouwmeester Dirk 
Published: October 2017

AbstractSystems of coupled mechanical resonators are useful for quantum information processing and fundamental tests of physics. Direct coupling is only possible with resonators of very similar frequency, but by using an intermediary optical mode, non-degenerate modes can interact and be independently controlled in a single optical cavity. Here we demonstrate coherent optomechanical state swapping between two spatially and frequency separated resonators with a mass ratio of 4. We find that, by using two laser beams far detuned from an optical cavity resonance, efficient state transfer is possible. Although the demonstration is classical, the same technique can be used to generate entanglement between oscillators in the quantum regime.
Atmospheric observations show accurate reporting and little growth in India’s methane emissions
Authors: Ganesan Anita L. , Rigby Matt , Lunt Mark F. , Parker Robert J. , Boesch Hartmut , Goulding N. , Umezawa Taku , Zahn Andreas , Chatterjee Abhijit , Prinn Ronald G. , Tiwari Yogesh K. , van der Schoot Marcel , Krummel Paul B. 
Published: October 2017

AbstractChanges in tropical wetland, ruminant or rice emissions are thought to have played a role in recent variations in atmospheric methane (CH4) concentrations. India has the world’s largest ruminant population and produces ~ 20% of the world’s rice. Therefore, changes in these sources could have significant implications for global warming. Here, we infer India’s CH4 emissions for the period 2010–2015 using a combination of satellite, surface and aircraft data. We apply a high-resolution atmospheric transport model to simulate data from these platforms to infer fluxes at sub-national scales and to quantify changes in rice emissions. We find that average emissions over this period are 22.0 (19.6–24.3) Tg yr−1, which is consistent with the emissions reported by India to the United Framework Convention on Climate Change. Annual emissions have not changed significantly (0.2 ± 0.7 Tg yr−1) between 2010 and 2015, suggesting that major CH4 sources did not change appreciably. These findings are in contrast to another major economy, China, which has shown significant growth in recent years due to increasing fossil fuel emissions. However, the trend in a global emission inventory has been overestimated for China due to incorrect rate of fossil fuel growth. Here, we find growth has been overestimated in India but likely due to ruminant and waste sectors.
Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity
Authors: Hu Lin , Xu Jiafeng , Xie Xiaomei , Zhou Yiwen , Tao Panfeng , Li Haidong , Han Xu , Wang Chong , Liu Jian , Xu Pinglong , Neculai Dante , Xia Zongping 
Published: October 2017

AbstractUbiquitin ligase TRAF6, together with ubiquitin-conjugating enzyme Ubc13/Uev1, catalyzes processive assembly of unanchored K63-linked polyubiquitin chains for TAK1 activation in the IL-1R/TLR pathways. However, what domain and how it functions to enable TRAF6’s processivity are largely uncharacterized. Here, we find TRAF6 coiled-coil (CC) domain is crucial to enable its processivity. The CC domain mediates TRAF6 oligomerization to ensure efficient long polyubiquitin chain assembly. Mutating or deleting the CC domain impairs TRAF6 oligomerization and processive polyubiquitin chain assembly. Fusion of the CC domain to the E3 ubiquitin ligase CHIP/STUB1 renders the latter capable of NF-κB activation. Moreover, the CC domain, after oligomerization, interacts with Ubc13/Ub~Ubc13, which further contributes to TRAF6 processivity. Point mutations within the CC domain that weaken TRAF6 interaction with Ubc13/Ub~Ubc13 diminish TRAF6 processivity. Our results reveal that the CC oligomerization primes its interaction with Ubc13/Ub~Ubc13 to confer processivity to TRAF6 ubiquitin ligase activity.
High-performance MnO2-deposited graphene/activated carbon film electrodes for flexible solid-state supercapacitor
Authors: Xu Lanshu , Jia Mengying , Li Yue , Jin Xiaojuan , Zhang Fan 
Published: October 2017

AbstractHigh theoretical capacitance of MnO2 nanoparticles were successfully electrodeposited on the conductive graphene/activated carbon (GN/AC) composite film, and the urchin type MnO2 microspheres were controlled by adjusting the electro-deposition reaction times. The GN/AC/MnO2-1200s composite electrodes exhibited a maximum specific capacitance of 1231 mF/cm2 (MnO2 loading mass of 7.65 mg/cm2 and the mass specific capacitance of 123 F/g) at a current density of 0.5 mA/cm2. The assembled flexible solid-state symmetric supercapacitor had a good mechanical flexibility (about 88.6% of its original capacitance after 500 bending times) and prominent cycling stability (about 82.8% retention in capacitance over 10000 cycles). More importantly, the device could possess a maximum energy density of 0.27 mW h/cm3 and a maximum power density of 0.02 W/cm3. These results well demonstrate a great potential for applications of GN/AC/MnO2 composite electrodes in flexible energy storage devices.
The application of thermophilic DNA primase TtDnaG2 to DNA amplification
Authors: Zhao D. , Chen Xiuqiang , Li Kuan , Fu Yu V. 
Published: October 2017

AbstractFor DNA replication in vivo, DNA primase uses a complementary single-stranded DNA template to synthesize RNA primers ranging from 4 to 20 nucleotides in length, which are then elongated by DNA polymerase. Here, we report that, in the presence of double-stranded DNA, the thermophilic DNA primase TtDnaG2 synthesizes RNA primers of around 100 nucleotides with low initiation specificity at 70 °C. Analysing the structure of TtDnaG2, we identified that it adopts a compact conformation. The conserved sites in its zinc binding domain are sequestered away from its RNA polymerase domain, which might give rise to the low initiation specificity and synthesis of long RNA segments by TtDnaG2. Based on these unique features of TtDnaG2, a DNA amplification method has been developed. We utilized TtDnaG2 to synthesize RNA primers at 70 °C after 95 °C denaturation, followed by isothermal amplification with the DNA polymerase Bst3.0 or phi29. Using this method, we successfully amplified genomic DNA of a virus with 100% coverage and low copy number variation. Our data also demonstrate that this method can efficiently amplify circular DNA from a mixture of circular DNA and linear DNA, thus providing a tool to amplify low-copy-number circular DNA such as plasmids.
Pulsed sputtering epitaxial growth of m-plane InGaN lattice-matched to ZnO
Authors: Kobayashi Atsushi , Ohta Jitsuo , Fujioka Hiroshi 
Published: October 2017

Abstractm-Plane GaN and InGaN films were grown on m-plane ZnO substrates at ~350 °C by pulsed sputtering deposition. It was found that the critical thickness of the m-plane GaN films grown on ZnO lies between 25 and 62 nm, whereas 180-nm-thick m-plane In0.12Ga0.88N can be coherently grown on ZnO substrates, which is explained well by theoretical calculations based on an energy-balance model. The coherently grown m-plane InGaN on ZnO exhibited narrow X-ray rocking curves compared with the m-plane GaN grown on ZnO. These results demonstrate the benefit of lattice-matched ZnO substrates for epitaxy of high-quality nonpolar InGaN films.
Dynamic mRNA and miRNA expression analysis in response to hypoxia and reoxygenation in the blunt snout bream (Megalobrama amblycephala)
Authors: Sun Shengming , Xuan Fujun , Ge Xianping , Zhu Jian , Zhang Wuxiao 
Published: October 2017

AbstractAdaptation to hypoxia is a complex process involving various pathways and regulation mechanisms. A better understanding of the genetic influence on these mechanisms could permit selection for hypoxia-sensitive fish. To aid this understanding, an integrated analysis of miRNA and mRNA expression was performed in Megalobrama amblycephala under four acute hypoxia and reoxygenation stages. A number of significantly differentially-expressed miRNAs and genes associated with oxidative stress were identified, and their functional characteristics were revealed by GO function and KEGG pathway analysis. They were found to be involved in HIF-1 pathways known to affect energy metabolism and apoptosis. MiRNA-mRNA interaction pairs were detected from comparison of expression between the four different stages. The function annotation results also showed that many miRNA-mRNA interaction pairs were likely to be involved in regulating hypoxia stress. As a unique resource for gene expression and regulation during hypoxia and reoxygenation, this study could provide a starting point for further studies to better understand the genetic background of hypoxia stress.
An exact solution for the free-vibration analysis of functionally graded carbon-nanotube-reinforced composite beams with arbitrary boundary conditions
Authors: Shi Zeyu , Yao Xiongliang , Pang Fuzhen , Wang Qingshan 
Published: October 2017

AbstractWe present an exact method to model the free vibration of functionally graded carbon-nanotube-reinforced composite (FG-CNTRC) beams with arbitrary boundary conditions based on first-order shear deformation elasticity theory. Five types of carbon nanotube (CNT) distributions are considered. The distributions are either uniform or functionally graded and are assumed to be continuous through the thickness of the beams. The displacements and rotational components of the beams are expressed as a linear combination of the standard Fourier series and several supplementary functions. The formulation is derived using the modified Fourier series and solved using the strong-form solution and the weak-form solution (i.e., the Rayleigh–Ritz method). Both solutions are applicable to various combinations of boundary constraints, including classical boundary conditions and elastic-supported boundary conditions. The accuracy, efficiency and validity of the two solutions presented are demonstrated via comparison with published results. A parametric study is conducted on the influence of several key parameters, namely, the L/h ratio, CNT volume fraction, CNT distribution, boundary spring stiffness and shear correction factor, on the free vibration of FG-CNTRC beams.
Rabies virus modifies host behaviour through a snake-toxin like region of its glycoprotein that inhibits neurotransmitter receptors in the CNS
Authors: Hueffer Karsten , Khatri Shailesh , Rideout Shane , Harris Michael B. , Papke Roger L. , Stokes Clare , Schulte Marvin K. 
Published: October 2017

AbstractRabies virus induces drastic behaviour modifications in infected hosts. The mechanisms used to achieve these changes in the host are not known. The main finding of this study is that a region in the rabies virus glycoprotein, with homologies to snake toxins, has the ability to alter behaviour in animals through inhibition of nicotinic acetylcholine receptors present in the central nervous system. This finding provides a novel aspect to virus receptor interaction and host manipulation by pathogens in general. The neurotoxin-like region of the rabies virus glycoprotein inhibited acetylcholine responses of α4β2 nicotinic receptors in vitro, as did full length ectodomain of the rabies virus glycoprotein. The same peptides significantly altered a nicotinic receptor induced behaviour in C. elegans and increased locomotor activity levels when injected into the central nervous system of mice. These results provide a mechanistic explanation for the behavioural changes in hosts infected by rabies virus.
Revealing hidden complexities of genomic rearrangements generated with Cas9
Authors: Boroviak Katharina , Fu Beiyuan , Yang Fengtang , Doe Brendan , Bradley Allan 
Published: October 2017

AbstractModelling human diseases caused by large genomic rearrangements has become more accessible since the utilization of CRISPR/Cas9 in mammalian systems. In a previous study, we showed that genomic rearrangements of up to one million base pairs can be generated by direct injection of CRISPR/Cas9 reagents into mouse zygotes. Although these rearrangements are ascertained by junction PCR, we describe here a variety of unanticipated structural changes often involving reintegration of the region demarcated by the gRNAs in the vicinity of the edited locus. We illustrate here some of this diversity detected by high-resolution fibre-FISH and conclude that extensive molecular analysis is required to fully understand the structure of engineered chromosomes generated by Cas9.
Oxidized g-C3N4/polyaniline nanofiber composite for the selective removal of hexavalent chromium
Authors: Kumar Rajeev , Barakat M. A. , Alseroury F. A. 
Published: October 2017

AbstractNanomaterials with selective adsorption properties are in demand for environmental applications. Herein, acid etching and oxidative decomposition of melon units of graphitic carbon nitride (g-C3N4) was performed to obtain the oxidized graphitic carbon nitride (Ox-g-C3N4) nanosheets. Ox- g-C3N4 nanosheets were further decorated on the polyaniline nanofiber (Ox-g-C3N4/Pani-NF). Ox-g-C3N4/Pani-NF was well characterized and further applied for a selective removal of hexavalent chromium (Cr(VI)) form aqueous solution. The zeta potential analysis indicate that the surface of Ox-g-C3N4/Pani-NF was positively charged which could be beneficial to bind anionic Cr(VI) ions electrostatically. In addition, nitrogen and oxygen containing functional groups exist on the Ox-g-C3N4/Pani-NF were mainly responsible for adsorption of Cr(VI) ions from aqueous solution. Moreover, the adsorption of Cr(VI) ions was also dependent on solution pH, reaction temperature and initial concentration of Cr(VI) ions. The maximum monolayer adsorption capacity of Ox-g-C3N4/Pani-NF for Cr(VI), calculated from Langmuir isotherm was 178.57 mg/g at pH = 2 and 30 °C. The activation energy (Ea = −20.66 kJ/mol) and the enthalpy change (ΔH° = −22.055 kJ/mol) validate the role of physical forces in adsorption of Cr(VI). These results demonstrate that Ox-g-C3N4/Pani-NF can be used as a potential adsorbent for environmental remediation applications.
Evaluation of water quality based on a machine learning algorithm and water quality index for the Ebinur Lake Watershed, China
Authors: Wang Xiaoping , Zhang Fei , Ding Jianli 
Published: October 2017

AbstractThe water quality index (WQI) has been used to identify threats to water quality and to support better water resource management. This study combines a machine learning algorithm, WQI, and remote sensing spectral indices (difference index, DI; ratio index, RI; and normalized difference index, NDI) through fractional derivatives methods and in turn establishes a model for estimating and assessing the WQI. The results show that the calculated WQI values range between 56.61 and 2,886.51. We also explore the relationship between reflectance data and the WQI. The number of bands with correlation coefficients passing a significance test at 0.01 first increases and then decreases with a peak appearing after 1.6 orders. WQI and DI as well as RI and NDI correlation coefficients between optimal band combinations of the peak also appear after 1.6 orders with R2 values of 0.92, 0.58 and 0.92. Finally, 22 WQI estimation models were established by POS-SVR to compare the predictive effects of these models. The models based on a spectral index of 1.6 were found to perform much better than the others, with an R2 of 0.92, an RMSE of 58.4, and an RPD of 2.81 and a slope of curve fitting of 0.97.
An eQTL variant of ZXDC is associated with IFN-γ production following Mycobacterium tuberculosis antigen-specific stimulation
Authors: Jabot-Hanin Fabienne , Cobat Aurélie , Feinberg Jacqueline , Orlova Marianna , Niay Jonathan , Deswarte Caroline , Poirier Christine , Theodorou Ioannis , Bustamante Jacinta , Boisson-Dupuis Stéphanie , Casanova Jean-Laurent , Alcaïs Alexandre , Hoal Eileen G. , Delacourt Christophe , Schurr Erwin , Abel Laurent 
Published: October 2017

AbstractThere is a large inter-individual variability in the response to Mycobacterium tuberculosis infection. In previous linkage analyses, we identified a major locus on chromosome region 8q controlling IFN-γ production after stimulation with live BCG (Bacillus Calmette-Guérin), and a second locus on chromosome region 3q affecting IFN-γ production triggered by the 6-kDa early secretory antigen target (ESAT-6), taking into account the IFN-γ production induced by BCG (IFNγ-ESAT6BCG). High-density genotyping and imputation identified ~100,000 variants within each linkage region, which we tested for association with the corresponding IFN-γ phenotype in families from a tuberculosis household contact study in France. Significant associations were replicated in a South African familial sample. The most convincing association observed was that between the IFNγ-ESAT6BCG phenotype and rs9828868 on chromosome 3q (p = 9.8 × 10−6 in the French sample). This variant made a significant contribution to the linkage signal (p < 0.001), and a trend towards the same association was observed in the South African sample. This variant was reported to be an eQTL of the ZXDC gene, biologically linked to monocyte IL-12 production through CCL2/MCP1. The identification of rs9828868 as a genetic driver of IFNγ production in response to mycobacterial antigens provides new insights into human anti-tuberculosis immunity.
Molecular Tilting Alignment on Ag@C Nanocubes Monitored by Temperature-Dependent Surface Enhanced Raman Scattering
Authors: Wang Yinong , Sun Yinghui , Chen Di , Zhang Xiaofang , Guo Lin , Wang Rongming 
Published: October 2017

AbstractCore@shell Ag@C nanocubes (NCs) with a cubic silver core (~60 nm of side length) and a coating of ultrathin amorphous carbon (~4 nm) have been synthesized on a large scale by a one-pot hydrothermal method. The carbon layer not only protects the Ag@C nanocubes from oxidation under hydrothermal condition, but also stabilizes the structure of Ag cores. Considering that optical properties of nanostructured metals strongly depend on the temperature for SERS measurement, in this work we systemically investigate the relationship between the orientation of molecules adsorbed on Ag@C NCs and temperature by SERS spectra. Results suggest that the adsorbed 4-MBA molecules prefer a flat orientation on the NC surface with temperature decreasing. In addition, Ag@C NCs after one-year storage in water still maintain high SERS-active capability. Our synthesized Ag@C NCs with excellent and stable optical properties can be potentially applied in the field of sensor and ultrasensitive spectral analysis.
Renal iron accumulation occurs in lupus nephritis and iron chelation delays the onset of albuminuria
Authors: Marks Eileen S. , Bonnemaison Mathilde L. , Brusnahan Susan K. , Zhang Wenting , Fan Wei , Garrison Jered C. , Boesen Erika I. 
Published: October 2017

AbstractProteins involved in iron homeostasis have been identified as biomarkers for lupus nephritis, a serious complication of systemic lupus erythematosus (SLE). We tested the hypothesis that renal iron accumulation occurs and contributes to renal injury in SLE. Renal non-heme iron levels were increased in the (New Zealand Black x New Zealand White) F1 (NZB/W) mouse model of lupus nephritis compared with healthy New Zealand White (NZW) mice in an age- and strain-dependent manner. Biodistribution studies revealed increased transferrin-bound iron accumulation in the kidneys of albuminuric NZB/W mice, but no difference in the accumulation of non-transferrin bound iron or ferritin. Transferrin excretion was significantly increased in albuminuric NZB/W mice, indicating enhanced tubular exposure and potential for enhanced tubular uptake following filtration. Expression of transferrin receptor and 24p3R were reduced in tubules from NZB/W compared to NZW mice, while ferroportin expression was unchanged and ferritin expression increased, consistent with increased iron accumulation and compensatory downregulation of uptake pathways. Treatment of NZB/W mice with the iron chelator deferiprone significantly delayed the onset of albuminuria and reduced blood urea nitrogen concentrations. Together, these findings suggest that pathological changes in renal iron homeostasis occurs in lupus nephritis, contributing to the development of kidney injury.
Fragmentation transitions in a coevolving nonlinear voter model
Authors: Min Byungjoon , Miguel Maxi San 
Published: October 2017

AbstractWe study a coevolving nonlinear voter model describing the coupled evolution of the states of the nodes and the network topology. Nonlinearity of the interaction is measured by a parameter q. The network topology changes by rewiring links at a rate p. By analytical and numerical analysis we obtain a phase diagram in p,q parameter space with three different phases: Dynamically active coexistence phase in a single component network, absorbing consensus phase in a single component network, and absorbing phase in a fragmented network. For finite systems the active phase has a lifetime that grows exponentially with system size, at variance with the similar phase for the linear voter model that has a lifetime proportional to system size. We find three transition lines that meet at the point of the fragmentation transition of the linear voter model. A first transition line corresponds to a continuous absorbing transition between the active and fragmented phases. The other two transition lines are discontinuous transitions fundamentally different from the transition of the linear voter model. One is a fragmentation transition between the consensus and fragmented phases, and the other is an absorbing transition in a single component network between the active and consensus phases.