bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streamlined screening platforms lead to the discovery of pachysiphine synthase from Tabernanthe iboga
Authors: Kamileen Mohamed O. , Nakamura Yoko , Luck Katrin , Heinicke Sarah , Hong Benke , Colinas Maite , Lichman Benjamin R. , O'Connor Sarah E. 
Published: September 2024

Summary

Plant‐specialized metabolism is largely driven by the oxidative tailoring of key chemical scaffolds catalyzed by cytochrome P450 (CYP450s) enzymes. Monoterpene indole alkaloids (MIAs) tabersonine and pseudo‐tabersonine, found in the medicinal plant Tabernanthe iboga (commonly known as iboga), are tailored with oxidations, and the enzymes involved remain unknown.

Here, we developed a streamlined screening strategy to test the activity of T. iboga CYP450s in Nicotiana benthamiana. Using multigene constructs encoding the biosynthesis of tabersonine and pseudo‐tabersonine scaffolds, we aimed to uncover the CYP450s responsible for oxidative transformations in these scaffolds.

Our approach identified two T. iboga cytochrome P450 enzymes: pachysiphine synthase (PS) and 16‐hydroxy‐tabersonine synthase (T16H). These enzymes catalyze an epoxidation and site‐specific hydroxylation of tabersonine to produce pachysiphine and 16‐OH‐tabersonine, respectively.

This work provides new insights into the biosynthetic pathways of MIAs and underscores the utility of N. benthamiana and Catharanthus roseus as platforms for the functional characterization of plant enzymes.
Photosynthetic ROS and retrograde signaling pathways
Authors: Lee Keun Pyo , Kim Chanhong 
Published: September 2024

SummarySessile plants harness mitochondria and chloroplasts to sense and adapt to diverse environmental stimuli. These complex processes involve the generation of pivotal signaling molecules, including reactive oxygen species (ROS), phytohormones, volatiles, and diverse metabolites. Furthermore, the specific modulation of chloroplast proteins, through activation or deactivation, significantly enhances the plant's capacity to engage with its dynamic surroundings. While existing reviews have extensively covered the role of plastidial retrograde modules in developmental and light signaling, our focus lies in investigating how chloroplasts leverage photosynthetic ROS to navigate environmental fluctuations and counteract oxidative stress, thereby sustaining primary metabolism. Unraveling the nuanced interplay between photosynthetic ROS and plant stress responses holds promise for uncovering new insights that could reinforce stress resistance and optimize net photosynthesis rates. This exploration aspires to pave the way for innovative strategies to enhance plant resilience and agricultural productivity amidst changing environmental conditions.
Food‐based dietary guidelines for optimizing calcium intakes for reproductive‐aged women in Ethiopia using local foods
Authors: Davies‐Kershaw Hilary , Tang Kevin , Gashu Dawd , Saje Semira Mitiku , Gomes Filomena , Joy Edward J. M. , Ander E. Louise , Gibson Sarah , Rana Ziaul H. , Ferguson Elaine L. 
Published: September 2024

AbstractIncreasing dietary calcium intakes of Ethiopian women of reproductive age (WRA) is a public health priority for reducing pre‐eclampsia in pregnancy. Using linear programming, we determined whether locally available foods consumed by WRA in nine regions (urban and rural) and two administrative cities of Ethiopia could provide 1000 mg/day of dietary calcium, and we identified food‐based recommendations (FBRs) to improve dietary calcium adequacy in each region. Results showed that diets providing 1000 mg/day of calcium were feasible in eight regions (40%) of the target populations examined. It would, however, require marked changes for most populations (90%), increasing the number of servings per week of several food groups to levels close to those of high consumers in each population. The selected calcium‐specific FBRs integrate well into the 2022 Ethiopian Dietary Guidelines, requiring additional messages to consume green leafy vegetables, milk, root crops, or teff (Eragrostis tef) or to consume a higher number of servings of vegetables than currently recommended, depending on the population. In conclusion, these analyses show that a food‐based approach can be used to achieve dietary calcium adequacy among WRA in 40% of the populations examined. For the other populations, food‐based interventions alone may be inadequate and other interventions are likely needed.
Kisspeptin in functional hypothalamic amenorrhea: Pathophysiology and therapeutic potential
Authors: Patel Aaran H. , Koysombat Kanyada , Pierret Aureliane , Young Megan , Comninos Alexander N. , Dhillo Waljit S. , Abbara Ali 
Published: September 2024

AbstractFunctional hypothalamic amenorrhea (FHA) is one of the most common causes of secondary amenorrhea, resulting in anovulation and infertility, and is a low estrogen state that increases the risk of cardiovascular disease and impairs bone health. FHA is characterized by acquired suppression of physiological pulsatile gonadotropin‐releasing hormone (GnRH) release by the hypothalamus in the absence of an identifiable structural cause, resulting in a functional hypogonadotropic hypogonadism. FHA results from either decreased energy intake and/or excessive exercise, leading to low energy availability and weight loss—often in combination with psychological stress on top of a background of genetic susceptibility. The hypothalamic neuropeptide kisspeptin is a key component of the GnRH pulse generator, tightly regulating pulsatile GnRH secretion and the downstream reproductive axis. Here, we review the physiological regulation of pulsatile GnRH secretion by hypothalamic kisspeptin neurons and how their activity is modulated by signals of energy status to affect reproductive function. We explore endocrine factors contributing to the suppression of GnRH pulsatility in the pathophysiology of FHA and how hypothalamic kisspeptin neurons likely represent a final common pathway through which these factors affect GnRH pulse generation. Finally, we discuss the therapeutic potential of kisspeptin as a novel treatment for women with FHA.
Rethinking City Population Growth: How Reclassification Matters
Authors: Alessandrini Alfredo , Deuster Christoph , Dijkstra Lewis , Ghio Daniela , Natale Fabrizio 
Published: September 2024

AbstractCity populations grow due to natural change, migration, and areas that are reclassified as part of a city. Because a time series of city boundaries was not available, most analyses ignore reclassification. This paper measures reclassification in a harmonized and transparent manner by applying a new harmonized definition of cities, towns, and rural areas, called the degree of urbanization, to gridded population data between 1980 and 2020. Ignoring reclassification would attribute city population growth equally to natural change and migration. Including the effects of reclassification reveals that two‐thirds of the growth is due to natural change, followed by reclassification (29 percent), and the remainder to migration (4 percent). This demonstrates the importance of accounting for reclassification. It also underlines that discouraging migration to cities will not significantly reduce city population growth.
Identification of risk loci for postpartum depression in a genome‐wide association study
Authors: Li Xue , Takahashi Nagahide , Narita Akira , Nakamura Yukako , Sakurai‐Yageta Mika , Murakami Keiko , Ishikuro Mami , Obara Taku , Kikuya Masahiro , Ueno Fumihiko , Metoki Hirohito , Ohseto Hisashi , Takahashi Ippei , Nakamura Tomohiro , Warita Noriko , Shoji Tomoka , Yu Zhiqian , Ono Chiaki , Kobayashi Natsuko , Kikuchi Saya , Matsuki Tasuku , Nagami Fuji , Ogishima Soichi , Sugawara Junichi , Hoshiai Tetsuro , Saito Masatoshi , Fuse Nobuo , Kinoshita Kengo , Yamamoto Masayuki , Yaegashi Nobuo , Ozaki Norio , Tamiya Gen , Kuriyama Shinichi , Tomita Hiroaki 
Published: September 2024

AimGenome‐wide association studies (GWAS) of postpartum depression (PPD) based on accumulated cohorts with multiple ethnic backgrounds have failed to identify significantly associated loci. Herein, we conducted a GWAS of Japanese perinatal women along with detailed confounding information to uncover PPD‐associated loci.MethodsThe first and second cohorts (n = 9260 and n = 8582 perinatal women enrolled in the Tohoku Medical Megabank Project) and the third cohort (n = 997), recruited at Nagoya University, underwent genotyping. Of them, 1421, 1264, and 225 were classified as PPD based on the Edinburgh Postnatal Depression Scale 1 month after delivery. The most influential confounding factors of genetic liability to PPD were selected, and logistic regression analyses were performed to evaluate genetic associations with PPD after adjusting for confounders.ResultsA meta‐analysis of GWAS results from the three cohorts identified significant associations between PPD and the following loci (P < 5 × 10−8) by integrating the number of deliveries and the number of family members living together as the most influential confounders: rs377546683 at DAB1, rs11940752 near UGT8, rs141172317, rs117928019, rs76631412, rs118131805 at DOCK2, rs188907279 near ZNF572, rs504378, rs690150, rs491868, rs689917, rs474978, rs690118, rs690253 near DIRAS2, rs1435984417 at ZNF618, rs57705782 near PTPRM, and rs185293917 near PDGFB. Pathway analyses indicated that SNPs suggestively associated with PPD were mostly over‐represented in categories including long‐term depression, GnRH signaling, glutamatergic synapse, oxytocin signaling, and Rap1 signaling.ConclusionThe current GWAS study identified eight loci significantly associated with PPD, which may clarify the genetic structure underlying its pathogenesis.
Zetetic supererogation
Authors: Hirvelä Jaakko 
Published: September 2024

AbstractSeveral authors have recently argued that knowledge is not the aim of inquiry since it can make sense to inquire into a question even though one knows the answer. I argue that this a faulty diagnostic for determining whether one has met the constitutive standard of success of an activity type. The constitutive standards of success tell us when an activity is successful, but such standards can be exceeded and exceeding them can be reasonable. To back this up I develop an account of zetetic supererogation and argue that subjects who continue to inquire into a question while knowing the answer do what is zetetically supererogatory.
Structural encroachment
Authors: Barranco Lopez Aliosha 
Published: September 2024

AbstractMoral encroachment states that moral factors can make a difference to what we are epistemically justified in believing. I present two motivating cases that resemble a common example in the moral encroachment literature to show that the agent's commitments and beliefs, and not the moral factors of the situation, influence epistemic justification. I call this view Structural Encroachment.
Mutation of KAP, which encodes a keratin‐associated protein, affects grain size and yield production in rice
Authors: Chen Chunpeng , Cheng Weimin , Jiang Hongrui , Fang Cheng , Li Wenhao , Peng Lingling , Tao Liangzhi , Zhan Yue , Wu Yuejin , Huang Xianzhong , Liu Binmei , Ye Yafeng 
Published: September 2024

AbstractGrain size and shape are critical agronomic traits that directly impact rice grain yield. Identifying genes that control these traits can provide new strategies for yield improvement. In this study, we characterized a rice mutant, reduced grain length (rgl), which exhibited decreased grain length due to reduced cell proliferation. Map‐based cloning identified a base deletion in OsRGL2, a gene encoding a keratin‐associated protein (KAP), as the cause of the mutant phenotype. CRISPR‐Cas9‐generated OsRGL2 knockout mutants also displayed reduced grain length, confirming its role. OsRGL2 transcripts were detected in various tissues, with relative higher gene expression in young panicles, and OsRGL2 was localized to the plasma membrane. Overexpression of OsRGL2 increased grain size by promoting cell proliferation in the spikelet hull and significantly enhanced grain yield per plant. Importantly, OsRGL2 was found to interact with RGB1, indicating that OsRGL2 positively regulates grain size and yield through its interaction with RGB1. Additionally, OsRGL2 regulated the expression of cell cycle‐related genes, further elucidating its role in grain development. These findings demonstrate that OsRGL2 is a positive regulator of grain size in rice, and manipulating its expression may offer a novel strategy for enhancing rice grain yield.
A Plant biostimulant prepared from Ascophyllum nodosum Induces Flowering by Regulating the MIR156‐mediated Age Pathway in Arabidopsis
Authors: Bahmani Ramin , Prithiviraj Balakrishnan 
Published: September 2024

AbstractFlowering, the change from vegetative development to the reproductive phase, represents a crucial and intricate stage in the life cycle of plants, which is tightly controlled by both internal and external factors. In this study, we investigated the effect of Ascophyllum nodosum extract (ANE) on the flowering time of Arabidopsis. We found that a 0.1% concentration of ANE induced flowering in Arabidopsis, accompanied by the upregulation of key flowering time genes: FT (FLOWERING LOCUS T), SOC1 (SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1), and LFY (LEAFY). Further investigation showed that ANE specifically promotes flowering through the MIR156‐mediated age pathway. ANE treatment resulted in the repression of negative regulator genes, MIR156, while simultaneously enhancing the expression of positive regulator genes, including SPLs and MIR172. This, in turn, led to the downregulation of AP2‐like genes, which are known as floral repressors. It is worth noting that ANE did not alleviate the late flowering phenotype of MIR156‐overexpressing plants and spl mutants. Furthermore, ANE‐derived fucoidan mimics the function of sugars in regulating MIR156, closely mirroring the effects induced by ANE treatments. It suppresses the transcript levels of MIR156 and AP2‐like genes while inducing those of SPLs and MIR172, thereby reinforcing the involvement of fucoidan in the control of flowering by ANE. In summary, our results demonstrate that ANE induces flowering by modulating the MIR156‐SPL module within the age pathway, and this effect is mediated by fucoidan.
Benefits of chest wall mobilization on respiratory efficiency and functional exercise capacity in people with severe chronic obstructive pulmonary disease (COPD): A randomized controlled trial
Authors: Tsui Amy Ying Yu , Cheing Gladys Lai Ying , Chau Rosanna Mei Wa , Mok Thomas Yun Wing , Ling Sai On , Kwan Candy Hoi Yee , Tsang Sharon Man Ha 
Published: September 2024

AbstractBackgroundCoexistence of chest wall hypomobility and lung hyperinflation compromises respiratory muscle function and respiratory efficiency in people with severe chronic obstructive pulmonary disease (COPD). This study aimed to investigate the effect of chest wall mobilization on functional exercise capacity, respiratory muscle activity and respiratory muscle tissue oxygen saturation for people with severe COPD.MethodsThirty male adults (age: 75 ± 6) diagnosed with severe COPD completed a 6‐week programme (twice/week) according to intervention randomization (chest wall mobilization group, CWMG, n = 15; control group, CG, n = 15). Both groups received standardized education and walking exercise, while CWMG also received chest wall and thoracic spine mobilization. Electromyography of the essential and accessory respiratory muscles and tissue oxygen saturation of the intercostal muscle (StO2, measured by near‐infrared spectroscopy) during incremental cycle exercise test were measured and compared between the two groups at pre‐programme, post‐programme and 3‐month follow‐up.ResultsPatients in CWMG demonstrated a significant increase in exercise tolerance from <3 METS to 4–6 METS (p = 0.000) after intervention. A significant decrease in activity of scalene, sternocleidomastoids and intercostal muscle during exercise test (p < 0.01) was found in CWMG, as compared to CG. A significant decrease in StO2 (p < 0.05) and greater decline in the slope of oxygenation dissociation (p = 0.000) were seen in CWMG during exercise test. These positive results were maintained at 3‐month follow‐up in CWMG.ConclusionImprovements in exercise tolerance, respiratory muscle efficiency and oxygenation extraction ability in CWMG suggest a potential clinical benefit of integrating chest wall and thoracic spine mobilization for rehabilitation of people with severe COPD.image
Inverse scattering transform for continuous and discrete space‐time‐shifted integrable equations
Authors: Ablowitz Mark J. , Musslimani Ziad H. , Ossi Nicholas J. 
Published: September 2024

AbstractNonlocal integrable partial differential equations possessing a spatial or temporal reflection have constituted an active research area for the past decade. Recently, more general classes of these nonlocal equations have been proposed, wherein the nonlocality appears as a combination of a shift (by a real or a complex parameter) and a reflection. This new shifting parameter manifests itself in the inverse scattering transform (IST) as an additional phase factor in an analogous way to the classical Fourier transform. In this paper, the IST is analyzed in detail for several examples of such systems. Particularly, time, space, and space‐time‐shifted nonlinear Schrödinger (NLS) and space‐time‐shifted modified Korteweg‐de Vries equations are studied. Additionally, the semidiscrete IST is developed for the time, space, and space‐time‐shifted variants of the Ablowitz–Ladik integrable discretization of the NLS. One‐soliton solutions are constructed for all continuous and discrete cases.
The role of White identity in anti‐racist allyship
Authors: Yantis Caitlyn 
Published: September 2024

AbstractAlthough White allies attempt to support racial justice movements, their behaviors can be interpreted as performative rather than genuine by racially minoritized individuals. In the current work, I discuss how White individuals' personal connection to their racial identity and the power it confers—White identity form—shapes their allyship behaviors. Specifically, moving beyond conceptualizations of White identity as centrality, I call for a study of White identity that incorporates power‐relevant ideologies, understanding how White individuals make meaning of their unearned privilege and incorporate it into their self‐concept. I suggest that a power‐cognizant identity form, which involves personally connecting the self to Whiteness and unearned power, is necessary for White individuals to engage in genuine anti‐racist allyship. I discuss White identity measurement issues as well as potential interventions for promoting a power‐cognizant identity form.
Not ready to make nice: Congressional candidates’ emotional appeals on Twitter
Authors: Russell Annelise , Evans Heather K. , Gervais Bryan 
Published: September 2024

AbstractObjectiveCongressional candidates use digital platforms to bolster and define their political reputation, and political stalemates over inflation, reproductive rights, and the lasting impact of Trump politics are fueling candidates’ emotionally charged rhetoric on Twitter, especially for women. Against the backdrop of President Donald Trump's presidency and the #MeToo movement, previous research has shown that women running for Congress are leading with angry rhetoric on Twitter. In this article, we ask whether anger is a persistent feature of women's digital appeals on Twitter over time.MethodUsing a data set of tweets by candidates for the U.S. House from 2016 to 2022, we highlight the escalating anger in the emotional appeals candidates make on Twitter and the resiliency of angry rhetoric as a modern feature of political Twitter.ResultsWe find that women, most notably Democratic candidates, are more likely to convey angry emotions on Twitter, not only matching male colleagues but defying gendered social stereotypes to turn frustration into a valuable political asset. Across the four last congressional elections, women have averaged more angry words in their digital appeals, with that anger as a consistent facet of how women engage online. Women are leaning into angry emotional appeals and adopting a negative appeal in their digital engagement that highlights their policy and political frustrations for voters.
Genre‐based writing in the German classroom in the age of generative AI
Authors: Redmann Jennifer 
Published: September 2024

AbstractGenre, defined as a “staged goal‐oriented social process” (Martin, 2009), has long stood as a key principle in second‐language writing instruction. This article presents the principles underpinning genre theory as it relates to the language curriculum and the five phases of genre‐based L2 writing pedagogy. This pedagogy provides the foundation for a case study in which students in an intermediate, fifth‐semester German class at a small liberal arts college received instruction in the film review genre. Student products written prior to classroom work with film reviews are compared with those written after students had gained an awareness and understanding of the film review genre, revealing the positive effects of genre‐based instruction on student writing. In light of the future impact of generative artificial intelligence (AI) on writing pedagogy, the final section addresses how ChatGPT and similar platforms can potentially enhance genre‐based pedagogies. The article suggests ways in which generative AI can be incorporated into each phase of genre‐based second language writing instruction. As technologies such as ChatGPT become established in language classrooms, it is clear that the writing process must share the stage with written products, both in instruction and assessments.
Semilinear clannish algebras
Authors: Bennett‐Tennenhaus Raphael , Crawley‐Boevey William 
Published: September 2024

AbstractWe define a class of associative algebras generalizing ‘clannish algebras’, as introduced by the second author, but also incorporating semilinear structure, like a skew polynomial ring. Clannish algebras generalize the well‐known ‘string algebras’ introduced by Butler and Ringel. Our main result is the classification of finite‐dimensional indecomposable modules for these new algebras.