bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a large-scale horizontal curve inventory for safety analysis using open GIS data
Authors: Ge Hanzhang , Qiu Shi , Hou Qing , Wang Jin , Ai Chengbo 
Published: October 2024

Abstract
Horizontal curves are crucial for the safety and mobility of roadways. To enhance the understanding of these curves, transportation agencies are investigating methods to accurately determine their spatial locations, geometric attributes, and conditions within their extensive road networks. Leveraging the widespread availability of Global Positioning System (GPS) and Geographic Information System (GIS) data, significant advancements have been made in developing automated algorithms that identify horizontal curves from GPS trajectories and GIS basemaps. This study introduces a novel dataset—the Horizontal Curve Inventory—that encapsulates detailed features of horizontal curves. We employed a modified automated cycle regression algorithm designed for large-scale road network analysis to extract horizontal curve data. This method was applied nationally to process an expansive network utilizing open-source GIS basemaps, encompassing over 300,000 miles across interstate, U.S. highway, and state highway systems. The inventory includes comprehensive data on curve radius, rotation angle, start and end point coordinates, and the curve’s center points. These data are essential for supporting safety research pertaining to road alignment. Our approach demonstrated robust performance across various open GIS data sources and provides the first accurate, efficient, and comprehensive inventory of horizontal curves for all major highway systems in the United States. The dataset is available at http://aichengbo.com/research/national-horizontal-curve-inventory/.
A search for water vapor plumes on Europa by spatially resolved spectroscopic observation using Subaru/IRCS
Authors: Kimura Jun , Matsuo Taro , Kobayashi Hitomi , Ikeda Yuji , Yoshioka Kazuo , Takagi Seiko , Ida Shigeru 
Published: October 2024

Abstract
We present near-infrared high-dispersion spectroscopic observations of Europa using the Infrared Camera and Spectrograph (IRCS) onboard the Subaru Telescope, seeking direct evidence of water plumes on Europa and exploring spatial variations in plume activity. Using the high spectral/spatial resolution and sensitivity of Subaru/IRCS, our observations have enabled a spatially resolved search for water plumes on Europa. Within our detection limits and time of observation, we found no evidence for the presence of water emission. For a rotation temperature of 50 K, we derived an upper limit on the H$_{2}$O abundance of 9.46 $\times$ 10$^{19}$–5.92 $\times$ 10$^{20}$ m$^{-2}$ in each divided slit area and 4.61 $\times$ 10$^{19}$ m$^{-2}$ in the entire area covered by the slit. This upper limit lies below the inferred water abundance from previous UV observations by the Hubble Space Telescope, while being less sensitive by a factor of three compared to the Keck telescope and by one order of magnitude or more than the James Webb Space Telescope observations. Our results align with previous studies and demonstrate that using Subaru/IRCS is an effective strategy for searching for water plumes on Europa with high spatial resolution. Continued observations across different surface areas and orbital phases are essential to fully characterize Europa’s plume activity and complement upcoming space missions.
The cliff-edge of toxicological concern: highlighting the potential issues of an over-reliance on “less-than-lifetime” thresholds
Authors: Waine Christopher J , Watts Peter , Hopkins James 
Published: October 2024

Abstract
The Threshold of Toxicological Concern (TTC) is a very well-established concept in applied toxicology, and has become a key tool for the pragmatic human health risk assessment of data-poor chemicals. Within the pharmaceutical sector, regulatory guidance on genotoxins defaults to a TTC of 1.5 μg/day equating to a maximum lifetime cancer risk of 1 in 100,000. Higher doses for drug products where exposures are intermittent or otherwise “less-than-lifetime” (LTL) are also considered tolerable. This also allows substance-specific lifetime Acceptable Intakes (AIs) for known genotoxic carcinogens to be scaled up for shorter durations.
The default TTCs for assessing LTL exposures build in conservatism such that there is deviation from strict linearity. However, close to the boundaries between LTL categories there can be such a difference in the default tolerable intakes that a health risk assessment can yield conflicting results. We have presented a theoretical case study based on our recent work that illustrates this apparent “cliff-edge.” The total acceptable cumulative dose over a 56-day treatment is – in absolute terms – one third of that allowed over 28 days, despite the maximum cancer risk of the longer exposure being an order of magnitude higher. Our analysis suggests the need for careful consideration of what might represent tolerable exposures in the region of the category limits, rather than simply adopting the hardline default. Where a potential patient exposure is found to be above a default value, there is real value in refining the cancer risk estimates using the Lifetime Cumulative Dose approach.
GABAergic Neurons in the Central Amygdala Promote Emergence from Isoflurane Anesthesia in Mice
Authors: Zhang Jin-Sheng , Yao Wei , Zhang Lei , Li Zhang-Shu , Gong Xia-Ting , Duan Li-Li , Huang Zhi-Xian , Chen Tong , Huang Jin-Chuang , Yang Shu-Xiang , Yu Changxi , Cai Ping , Chen Li 
Published: October 2024

Background:
Recent evidence indicates that general anesthesia and sleep–wake behavior share some overlapping neural substrates. γ-Aminobutyric acid–mediated (GABAergic) neurons in the central amygdala have a high firing rate during wakefulness and play a role in regulating arousal-related behaviors. The objective of this study was to investigate whether central amygdala GABAergic neurons participate in the regulation of isoflurane general anesthesia and uncover the underlying neural circuitry.

Methods:
Fiber photometry recording was used to determine the changes in calcium signals of central amygdala GABAergic neurons during isoflurane anesthesia in Vgat-Cre mice. Chemogenetic and optogenetic approaches were used to manipulate the activity of central amygdala GABAergic neurons, and a righting reflex test was used to determine the induction and emergence from isoflurane anesthesia. Cortical electroencephalogram (EEG) recording was used to assess the changes in EEG spectral power and burst–suppression ratio during 0.8% and 1.4% isoflurane anesthesia, respectively. Both male and female mice were used in this study.

Results:
The calcium signals of central amygdala GABAergic neurons decreased during the induction of isoflurane anesthesia and were restored during the emergence. Chemogenetic activation of central amygdala GABAergic neurons delayed induction time (mean ± SD, vehicle vs. clozapine-N-oxide: 58.75 ± 5.42 s vs. 67.63 ± 5.01 s; n = 8; P = 0.0017) and shortened emergence time (385.50 ± 66.26 s vs. 214.60 ± 40.21 s; n = 8; P = 0.0017) from isoflurane anesthesia. Optogenetic activation of central amygdala GABAergic neurons produced a similar effect. Furthermore, optogenetic activation decreased EEG delta power (prestimulation vs. stimulation: 46.63 ± 4.40% vs. 34.16 ± 6.47%; n = 8; P = 0.0195) and burst–suppression ratio (83.39 ± 5.15% vs. 52.60 ± 12.98%; n = 8; P = 0.0003). Moreover, optogenetic stimulation of terminals of central amygdala GABAergic neurons in the basal forebrain also promoted cortical activation and accelerated behavioral emergence from isoflurane anesthesia.

Conclusions:
The results suggest that central amygdala GABAergic neurons play a role in general anesthesia regulation, which facilitates behavioral and cortical emergence from isoflurane anesthesia through the GABAergic central amygdala–basal forebrain pathway.
Investigating the link between coronary artery calcification and atrial fibrillation: A systematic review and meta-analysis
Authors: Tabesh Faezeh , Arjmand Zahra , Teimouri-Jervekani Zahra 
Published: October 2024

Background:
Both coronary artery calcification (CAC) and atrial fibrillation (AF) are associated with common risk factors, such as age, hypertension, diabetes, and dyslipidemia. Investigating their association may provide insights into shared pathophysiological mechanisms and overlapping risk profiles.

Methods:
A systematically search was performed covering various data bases including PubMed, Scopus, ISI, and Google Scholar from January 2000 until December 2023 for English-published articles. We focused on observational studies—cohort, case–control, and cross-sectional—that involved adult participants (aged 18 and older) and provided data on the any association between CAC and AF. We excluded studies if the full text was unavailable, the publication was not in English, there was insufficient numerical data or relative risk information.

Results:
All the included articles (n = 14), were observational studies (n = 5 case–control and n = 9 cohort). All of the included papers (100%), reported a link between CAC and higher rate of major adverse cardiovascular events such as stroke and mortality in AF patients. The overall prevalence of AF across 5 studies was estimated to be 9.00% (95% confidence intervals [CI]: 2.00, 16.00), and 60% of the AF population were male. The overall proportion of AF in individuals with a coronary artery calcification score (CACS) of 0 is relatively high at 0.48 (95% CI: 0.32, 0.64), followed by CACS (1–99) which was 0.22 (95% CI: 0.16, 0.28), CACS (100–399) which was 0.17 (95% CI: 0.07, 0.28), and CACS (≥ 400) as 0.14 (95% CI: 0.07, 0.22).

Conclusion:
It can be concluded that there is a robust and consistent association between CAC and AF. Our systematic review underscores the importance of considering CAC as a potential factor in assessing the risk of AF, while also recognizing the multifactorial nature of this relationship.
Lamotrigine associated extensive hyperpigmentation: A case report and literature review
Authors: Pei Keling , Wu Yuqian , Zhang Tao 
Published: October 2024

Rationale:
Lamotrigine (LTG)-associated extensive hyperpigmentation is rare and may persist for a long time or even become permanent. LTG-associated cutaneous adverse reaction (CAR) manifests initially as mild maculopapular exanthema (MPE). The first step in CAR therapy is to immediately discontinue the offending LTG for predicting evolution to mild or severe forms not always possible. Here, we present a rare case of LTG-associated extensive hyperpigmentation for delaying the withdrawal of LTG.

Patient concerns:
We describe the case of a female adolescent with a history of depression managed with LTG, who developed a mild MPE. Unfortunately, the patient did not discharge LTG after the occurrence of MPE until 20 days later. Then she developed a residual extensive hyperpigmentation in her trunk and extremities.

Diagnoses:
After a series of physical examinations and retracing past medical history, she was diagnosed with LTG-associated extensive hyperpigmentation.

Interventions:
The patient refused any treatment.

Outcomes:
Nine months later, there still existed residual hyperpigmentation in her trunk and extremities, and the range and color of hyperpigmentation have not changed significantly.

Lessons:
This case suggests that LTG may cause not only MPE but also extensive hyperpigmentation. When a patient displays a mild MPE following the initiation of LTG in the outpatient clinic, LTG-associated CAR should not be overlooked as a diagnosis, and early withdrawal of LTG should be considered at first.
Network meta-analysis evaluating the impact of diverse exercise regimens on quality of life in women post-breast cancer surgery
Authors: Yao Lin-Xi , Zhou Yan-Bing , Yuan Yu-Xin , Li Xiao-Fei , Li Xiao-Fen 
Published: October 2024

Background:
This study used network meta-analysis to compare the effects of various exercise interventions, with the aim of identifying the most effective intervention measures.

Methods:
Data were extracted from 8 databases, comprising of PubMed, Web of Science, EBSCO, and Embase. The Cochrane risk of bias assessment tool was used to assess the quality of the research. Network meta-analysis and meta-analysis were performed using STATA15.0 and Review Manager 5.4, respectively.

Results:
This analysis incorporated 58 research papers that had 4998 participants, of which 2480 were assigned to the test group and 2518 to the control group. As revealed by the data, the following is a descending order of the impact of various exercise regimens on the quality of life (QoL) of women who have undergone breast cancer surgery: For the MOS item short form health survey, the recommended sequence was as follows: aerobic + resistance exercise, football, baduanjin, walking, tai chi, yoga, resistance exercise, slow-moving qigong, aerobic exercise, and usual care; for functional assessment of cancer therapy for breast cancer, the sequence was as follows: aerobic + resistance exercise, resistance exercise, relaxation, baduanjin, square dance, aerobic exercise, tai chi, yoga, Pilates, mixer dance, aquatic sports, cycle ergometer, and usual care; and for European Organization for Research and Treatment of Cancer quality of life questionnaire, the sequence was aerobic + resistance exercise, aerobic + yoga, aerobic exercise, Pilates, yoga, muscle strengthening, resistance exercise, square dance, usual care, walking, and aerobic + edema massage.

Conclusion:
The majority of exercise interventions demonstrated a discernible impact on the QoL in women following breast cancer surgery. The effects of diverse exercise interventions on the 3 QoL outcome indicators in patients with breast cancer exhibited variability. However, irrespective of the QoL measurement technique used, aerobic and resistance exercise continue to be the most favorable choices.
Mitochondrial diabetes presenting with spontaneous abortion and ketoacidosis onset: A case report and literature review
Authors: Wu Baoyuan , Tao Yubing , Wu Qingqiang , Zou Caiyan , Liu Xuekui , Geng Houfa 
Published: October 2024

Rationale:
Mitochondrial diabetes mellitus (MDM) is a rare form of diabetes characterized by mitochondrial dysfunction, leading to a diverse range of clinical manifestations that may result in misdiagnosis. Accurate identification of MDM is essential for proper management and reporting of diagnosed cases.

Patient concerns:
The patient was a young woman with a slender physique who presented with sensorineural hearing loss detected during auditory testing.

Diagnoses:
Auditory testing confirmed sensorineural hearing loss, and further evaluation revealed impaired pancreatic β-cell function, indicating reduced insulin secretion. Genetic testing of blood samples identified the A3243G mitochondrial DNA mutation. The patient’s family history was notable for hearing loss in her mother and maternal grandmother, who exhibited clinical features consistent with MDM.

Interventions:
Clinical management focused on monitoring and addressing the metabolic and clinical needs associated with MDM.

Outcomes:
The diagnosis of MDM was established, highlighting the importance of recognizing the diverse clinical manifestations, including a rare case of spontaneous abortion during pregnancy.

Lessons:
MDM presents with atypical clinical manifestations, and thorough physical examinations are crucial for its diagnosis. This case underscores the significance of genetic testing and family history in diagnosing MDM and the need for increased awareness among clinicians to prevent misdiagnosis.
A bibliometric analysis and typology of drug pricing policies across the globe
Authors: Berthe Abdrahmane , Eljilany Islam , Kulkarni Amit , Diaby Vakaramoko 
Published: October 2024

Background:
Limited research exists on pricing policies from a bibliometric and visualization perspective, and there is a lack of understanding of their typology. This study aims to address these gaps in knowledge and provide a deeper understanding of the research topics and development trends in this field.

Methods:
A bibliometric study was conducted on drug pricing approaches in healthcare literature, published between 2000 and June 2023. The literature was identified through an extensive search of healthcare databases and was then classified based on the year of publication, research topics, corresponding authors, location of corresponding authors, and journal titles. The citation data analysis was conducted using Bibliometrix, which consisted of descriptive, geographical, and time-series analyses and visualization.

Results:
Between 2000 and June 2023, 173 articles were disseminated across 98 distinct publication sources. During the analysis, we observed a significant and consistent rise in literature reports on drug pricing approaches in healthcare, especially in 2010. The research topics were distributed almost equally, discussing improvement or issues with drug pricing models and addressing drug pricing applications. Our analysis revealed that the top ten corresponding authors were responsible for 19% of the total articles, with those based in the United States being the most productive. Furthermore, the “Health Economics” journal ranked first among the top ten journals. These findings align with the overall publication trends of drug pricing methods reported in other fields.

Conclusions:
The current study offers a comprehensive overview of drug pricing techniques utilized in medicine through visualization and bibliometric techniques. Analysis of authors, journals, institutions, and countries could serve as a reference for new researchers and guide them differently. Researchers can also consider emerging trends when determining the focus of their studies.
Sex bias in FGFR3 somatic mutations in bladder cancer
Authors: Meng Xiangyu , Wang Qiaoli 
Published: October 2024

Abstract

Background
Strong sex disparities have been observed among patients with bladder cancer (BCa). FGFR3 is one of the most frequently mutated genes in bladder cancer, and there are inconsistencies in its frequency in male and female patients.

Methods
Here, we conducted a meta-analysis comparing the FGFR3 somatic mutation frequency in men and women among 7351 patients with BCa from 18 cohorts.

Results
We showed that female patients had a 1.32 times higher risk of having FGFR3 somatic mutations than males. This difference was attributed to mutations occurring at the 2 most frequently mutated sites, S249 and Y375. Additionally, nonsense mutations were more likely to be found in women, whereas indel/frameshift mutations were almost exclusively found in men; however, no difference was noted for missense mutations.

Conclusions
A female sex bias in FGFR3 somatic mutations was observed in BCa. Well-powered individual participant data analyses addressing the possible confounding effects of other factors (eg, age, ethnicity, smoking status, muscle invasiveness, and molecular subtype), as well as analyses integrating omics and functional investigations, are warranted to further validate and explain the mechanisms of the current findings.
Successful Collaborations that Resulted in Increased U.S. Diagnostic Testing During the 2022 Mpox Outbreak
Authors: Hutson Christina L. , Villanueva Julie , Stenzel Timothy , Olson Victoria A. , Gerald Noel , McNall Rebecca , Courtney Sean , Aden Tricia , Rager Stacy , Egan Christina , Blevins Patricia , Kuhnert Wendi , Davidson Whitni , Khan Theodora , Baird Nicolle , Kling Chantal , Van Meter Susan , Chaitram Jasmine , Salerno Reynolds M. 
Published: October 2024

Context
The first case of mpox was detected in the United States in a Laboratory Response Network (LRN) laboratory at the Massachusetts Department of Public Health on May 17, 2022. Through previous years of smallpox preparedness efforts by the United States government, testing capacity in LRN laboratories across the United States utilizing the FDA-cleared Centers for Disease Control and Prevention (CDC) Non-variola orthopoxvirus (NVO) test was approximately 6000 tests weekly across the nation prior to the mpox outbreak. By early June 2022, the LRN laboratories had capacity to perform up to 8000 tests per week. As the outbreak expanded, cases were identified in every United States state, peaking at ~3000 cases per week nationally in August 2022.

Objective
Although NVO testing capacity in LRN laboratories exceeded national mpox testing demand overall, LRN testing access in some areas was challenged and test expansion was necessary.

Participants
CDC engaged with partners and select commercial laboratories early to increase diagnostic testing access by allowing these commercial laboratories to utilize the NVO test.

Setting
The expansion of testing to commercial laboratories increased testing availability, capacity, and volume nationwide. This was the first time that CDC shared an FDA 510k-cleared molecular test with commercial laboratories to support a public health emergency.

Design
Extensive efforts were made to ensure the CDC NVO test was used appropriately in the private sector and that the transfer process met regulatory requirements.

Main Outcome Measures, Results, Conclusions
These novel methods to expand NVO testing to commercial laboratories increased national testing capacity to 80 000 mpox tests/week. Test volumes among these laboratories never exceeded this expanded capacity. The rapid increase in the nation’s testing capacity, in conjunction and coordination with other public and private health efforts, helped to detect cases rapidly. These actions demonstrated the importance of highly functional and efficient public health and private sector partnerships for responding to public health emergencies.
Simple Trocar Placement for Robotic Liver and Pancreatic Surgery: Multiple Access Devices at 5-cm Single Umbilicus Incision with 2 Robotic Ports
Authors: Kawaguchi Yoshikuni , Ito Kyoji , Abe Satoru , Nishioka Yujiro , Miyata Akinori , Ichida Akihiko , Akamatsu Nobuhisa , Hasegawa Kiyoshi 
Published: October 2024

Background:
Robotic trocars are recommended to be placed 6–8 cm apart, and assistant trocars are placed 7 cm away from the horizontal line of the robotic trocar placement. However, adhering to these rules may be difficult, particularly in lean patients. This study aims to demonstrate our standardized simple trocar placement, 5-cm single umbilicus incision + 2 ports for robotic liver resection (RLR) and robotic pancreaticoduodenectomy (RPD).

Methods:
During 2022–2024, patients undergoing RLR and RPD at The University of Tokyo were identified from a prospectively maintained database. Our standardized trocar placement consisted of multiple access devices at the 5-cm umbilicus incision, including two robotic trocars and one assistant trocar, and two other robotic trocars inserted to shape a hemi-circle around the surgical object.

Results:
A total of 29 and 31 consecutive patients underwent RLR and RPD, respectively. The 5-cm single umbilicus incision + 2 ports method was used in 82.8% of patients undergoing RLR and 100% of patients undergoing RPD. No patients undergoing RLR developed hepatic insufficiency and bile leakage with the median postoperative hospital stay of 5 days. No patients undergoing RPD developed clinically relevant pancreatic fistula, delayed gastric emptying, and bile leakage with the median postoperative hospital stay of 8 days.

Conclusions:
Our standardized trocar placement technique is simple and widely feasible for lean-to-obese patients, allowing for RLR and RPD while minimizing incisions for patients.