bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observing and Predicting the 2015/16 El Niño
Authors: L’Heureux Michelle L. , Takahashi Ken , Watkins Andrew B. , Barnston Anthony G. , Becker Emily J. , Di Liberto Tom E. , Gamble Felicity , Gottschalck Jon , Halpert Michael S. , Huang Boyin , Mosquera-Vásquez Kobi , Wittenberg Andrew T. 
Published: November 2016

Abstract
The El Niño of 2015/16 was among the strongest El Niño events observed since 1950 and took place almost two decades after the previous major event in 1997/98. Here, perspectives of the event are shared by scientists from three national meteorological or climate services that issue regular operational updates on the status and prediction of El Niño–Southern Oscillation (ENSO). Public advisories on the unfolding El Niño were issued in the first half of 2015. This was followed by significant growth in sea surface temperature (SST) anomalies, a peak during November 2015–January 2016, subsequent decay, and its demise during May 2016. The life cycle and magnitude of the 2015/16 El Niño was well predicted by most models used by national meteorological services, in contrast to the generally overexuberant model predictions made the previous year. The evolution of multiple atmospheric and oceanic measures demonstrates the rich complexity of ENSO, as a coupled ocean–atmosphere phenomenon with pronounced global impacts. While some aspects of the 2015/16 El Niño rivaled the events of 1982/83 and 1997/98, we show that it also differed in unique and important ways, with implications for the study and evaluation of past and future ENSO events. Unlike previous major El Niños, remarkably above-average SST anomalies occurred in the western and central equatorial Pacific but were milder near the coast of South America. While operational ENSO systems have progressed markedly over the past several decades, the 2015/16 El Niño highlights several challenges that will continue to test both the research and operational forecast communities.
A lost track in ICHD 3 beta: A comprehensive review on osmophobia
Authors: Zanchin Giorgio , Fuccaro Matteo , Battistella Pierantonio , Ermani Mario , Mainardi Federico , Maggioni Ferdinando 
Published: November 2016

Background Osmophobia (Os) has been reported to be much more prevalent in migraine (M) than in other primary headaches, and its high specificity in the differential diagnosis between M and tension-type headache (TTH) has been reported. Os was included in the ICHD II Appendix as a diagnostic criterion of M. It disappeared in ICHD-3 beta. To understand this choice, we reviewed the literature after 2004. Methods This was a systematic review. We searched in PubMed, MEDLINE and Cochrane library for “osmophobia”, “odour/odorphobia AND headache”, “odour/odor hypersensitivity AND headache” and “olfactory hypersensitivity AND headache”. Results 112 papers cited Os as an accompanying symptom of headache; 16 focused on Os in M diagnosis. With the data from 40 articles, we calculated the pooled prevalence of Os in 14,360 patients (2281 pediatric) affected by M (n = 12,496) and TTH (n = 1864). In M, the prevalence was 48.5% (CI 95% 41.4 to 55.8%) in adults and 23.4% (CI 95% 15.7 to 33.4%) in pediatric patients; in TTH, the prevalence was 8.9% (CI 95% 4.6 to 13.5%) in adults and 7.9% (CI 95% 3.3 to 18.1%) in pediatric patients. Ten of these papers allowed us to calculate the sensibility and specificity of Os in differential diagnosis between M and TTH. In adults, the value of specificity was 94.1% (CI 95% 88.9 to 96.9%), and sensitivity was 51.4% (CI 95% 38.4 to 64.2%). In pediatric patients, specificity was 92.0% (CI 95% 81.9 to 96.7%), and sensitivity was 22.1% (CI 95% 10.1 to 41.8%). Conclusion The literature endorses the inclusion of Os among M diagnostic criteria. On this ground, the decision to remove Os from ICHD 3 beta appears unjustified and a revision of this choice is recommended.
Trigger points are associated with widespread pressure pain sensitivity in people with tension-type headache
Authors: Palacios-Ceña María , Wang Kelun , Castaldo Matteo , Guillem-Mesado Amparo , Ordás-Bandera Carlos , Arendt-Nielsen Lars , Fernández-de-las-Peñas César 
Published: November 2016

Objective To investigate the association between trigger points (TrPs) and widespread pressure pain sensitivity in people with tension-type headache (TTH) and to determine if this association is different between frequent episodic (FETTH) or chronic (CTTH) headache. Design A cross-sectional study. Methods One hundred and fifty-seven individuals (29% male) with TTH participated. Clinical features of headache, i.e., intensity, duration, and frequency, were recorded in a headache diary. Active and latent TrPs were bilaterally explored in the temporalis, masseter, suboccipital, upper trapezius, sternocleidomastoid, and splenius capitis muscles. Pressure pain thresholds (PPT) were assessed over the trigeminal area (i.e., temporalis muscle), extra-trigeminal (i.e., C5/C6 zygapophyseal joint), and two distant pain-free points (i.e., second metacarpal and tibialis anterior muscle). Results Eighty (51%) patients were classified as FETTH, whereas 77 (49%) were classified as CTTH. No differences in the number of either active or latent TrPs (all p > 0.171) or widespread pressure pain sensitivity (all p > 0.351) were observed between FETTH and CTTH groups. The number of active and latent TrPs was significantly and negatively associated with PPTs: The higher the number of active or latent TrPs, the lower the widespread PPT, and the more generalized sensitization. This association was stronger within the FETTH group than the CTTH group. Conclusions This study found that the number of TrPs in head and neck/shoulder muscles was associated with widespread pressure hypersensitivity independently of the frequency of headache.
Sexual functioning in multiple sclerosis: Relationships with depression, fatigue and physical function
Authors: Young Carolyn A , Tennant Alan ,   on Behalf of the TONiC Study Group, Mills RJ , Rog DJ , Ford HL , Orchard K 
Published: November 2016

Background: Sexual dysfunction (SD) is common among people with multiple sclerosis, but there is limited information on its relationships. Objective: This national study examines the relationships between sexual function with demographic factors, physical function, fatigue and depression. Methods: Participants in the Trajectories of Outcomes in Neurological Conditions (TONiC) study completed the measures of fatigue, physical function and depression together with the Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15), which covers sexual function (primary), symptoms that interfere with sexual function (secondary) and psychological issues (tertiary). All ordinal scores from the measures were converted to interval scale latent estimates via the Rasch model and used as single indicator latent variables in path analysis. Results: From 722 patients, 538 were sexually active of whom 431 (80.1%) answered questions on sexual functioning. Of these, only 18.5% reported no impact on any aspect of sexual function. Dysfunction was linked to disease subtype and Expanded Disability Status Scale (EDSS). Subtype influenced the relationship between symptoms and sexual function, which was fully mediated by psychological factors in secondary progressive patients. Depression was not directly associated with sexual functioning, but appeared as a consequence of the psychological issues associated with SD. Conclusion: SD is common among participants in this UK-wide study, and psychological aspects of SD contributed to depression.
Patient characteristics related to metabolic disorders and chronic complications in type 2 diabetes mellitus patients hospitalized at the Qingdao Endocrine and Diabetes Hospital from 2006 to 2012 in China
Authors: Dong Yanhu , Gao Weiguo , Zhang Lei , Wei Jia , Hammar Niklas , Cabrera Claudia S , Wu Xiaoli , Qiao Qing 
Published: November 2016

Objective: To study the clinical characteristics related to metabolic disorders and complications in type 2 diabetes mellitus patients hospitalized in the Qingdao Endocrine and Diabetes Hospital from 2006 to 2012 in Qingdao, China. Patient population and methods: Data from 14,289 (51% males) type 2 diabetes mellitus patients hospitalized between 2006 and 2012 were collected and analysed. Information on patients’ demographic, anthropometric, laboratory and disease histories were extracted from electronic medical records. Results: The mean age of the patients was 60.5 years, with median diabetes duration of 9.0 years. Mean haemoglobin A1c was 8.4%, where <30.0% of patients had haemoglobin A1c <7.0%. In all, 34.5% of patients had low-density lipoprotein cholesterol lower than 2.6 mmol/L and 31.9% had hypertriglyceridaemia. Retinopathy was diagnosed in 51.1% of patients, nephropathy in 21.6%, neuropathy in 50.4%, hypertension in 77.4%, coronary heart disease in 27.6% and peripheral vascular disease in 58.6%. Once hospitalized, 71.1% of patients underwent insulin injection treatments, either mono-therapy or combined with other anti-diabetic drugs. Metformin was prescribed to 36.9% of patients, followed by acarbose 29.9%, thiazolidinediones 18.1%, meglitinides 14.8% and sulfonylureas 10.7%. Conclusion: Inadequate control of hyperglycaemia, poor metabolic profiles and diabetic complications were common challenges for long-term diabetes management in Chinese patients with type 2 diabetes mellitus.
Rare variants in GP1BB are responsible for autosomal dominant macrothrombocytopenia
Authors: Sivapalaratnam Suthesh , Westbury Sarah K. , Stephens Jonathan C. , Greene Daniel , Downes Kate , Kelly Anne M. , Lentaigne Claire , Astle William J. , Huizinga Eric G. , Nurden Paquita , Papadia Sofia , Peerlinck Kathelijne , Penkett Christopher J. , Perry David J. , Roughley Catherine , Simeoni Ilenia , Stirrups Kathleen , Hart Daniel P. , Tait R. Campbell , Mumford Andrew D. ,   NIHR BioResource, Laffan Michael A. , Freson Kathleen , Ouwehand Willem H. , Kunishima Shinji , Turro Ernest 
Published: November 2016

Key PointsVariants in GP1BB can cause autosomal dominant macrothrombocytopenia.
Outcomes measures in a decade of dementia and mild cognitive impairment trials
Authors: Harrison Jennifer Kirsty , Noel-Storr Anna H. , Demeyere Nele , Reynish Emma L. , Quinn Terry J. 
Published: November 2016

Abstract
Background
In a research study, to give a comprehensive evaluation of the impact of interventions, the outcome measures should reflect the lived experience of the condition. In dementia studies, this necessitates the use of outcome measures which capture the range of disease effects, not limited to cognitive functioning. In particular, assessing the functional impact of cognitive impairment is recommended by regulatory authorities, but there is no consensus on the optimal approach for outcome assessment in dementia research. Our aim was to describe the outcome measures used in dementia and mild cognitive impairment (MCI) intervention studies, with particular interest in those evaluating patient-centred outcomes of functional performance and quality of life.

Methods
We performed a focused review of the literature with multiple embedded checks of internal and external validity. We used the Cochrane Dementia and Cognitive Improvement Group’s register of dementia studies, ALOIS. ALOIS was searched to obtain records of all registered dementia and MCI intervention studies over a 10-year period (2004–2014). We included both published and unpublished materials. Outcomes were categorised as cognitive, functional, quality of life, mood, behaviour, global/disease severity and institutionalisation.

Results
From an initial return of 3271 records, we included a total of 805 records, including 676 dementia trial records and 129 MCI trial records. Of these, 78 % (630) originated from peer-reviewed publications and 60 % (487) reported results of pharmacological interventions. Cognitive outcomes were reported in 70 % (563), in contrast with 29 % (237) reporting measures of functional performance and only 13 % (102) reporting quality of life measures. We identified significant heterogeneity in the tools used to capture these outcomes, with frequent use of non-standardised tests.

Conclusions
This focus on cognitive performance questions the extent to which intervention studies for dementia are evaluating outcome measures which are relevant to individual patients and their carers. The heterogeneity in measures, use of bespoke tools and poor descriptions of test strategy all support the need for a more standardised approach to the conduct and reporting of outcomes assessments.
Calcium homeostasis alterations in a mouse model of the Dynamin 2-related centronuclear myopathy
Authors: Fraysse Bodvaël , Guicheney Pascale , Bitoun Marc 
Published: November 2016

ABSTRACT
Autosomal dominant centronuclear myopathy (CNM) is a rare congenital myopathy characterized by centrally located nuclei in muscle fibers. CNM results from mutations in the gene encoding dynamin 2 (DNM2), a large GTPase involved in endocytosis, intracellular membrane trafficking, and cytoskeleton regulation. We developed a knock-in mouse model expressing the most frequent DNM2-CNM mutation; i.e. the KI-Dnm2R465W model. Heterozygous (HTZ) KI-Dnm2 mice progressively develop muscle atrophy, impairment of contractile properties, histopathological abnormalities, and elevated cytosolic calcium concentration. Here, we aim at better characterizing the calcium homeostasis impairment in extensor digitorum longus (EDL) and soleus muscles from adult HTZ KI-Dnm2 mice. We demonstrate abnormal contractile properties and cytosolic Ca2+ concentration in EDL but not soleus muscles showing that calcium impairment is correlated with muscle weakness and might be a determinant factor of the spatial muscle involvement. In addition, the elevated cytosolic Ca2+ concentration in EDL muscles is associated with an increased sarcolemmal permeability to Ca2+ and releasable Ca2+ content from the sarcoplasmic reticulum. However, amplitude and kinetics characteristics of the calcium transient appear unchanged. This suggests that calcium defect is probably not a primary cause of decreased force generation by compromised sarcomere shortening but may be involved in long-term deleterious consequences on muscle physiology. Our results highlight the first pathomechanism which may explain the spatial muscle involvement occurring in DNM2-related CNM and open the way toward development of a therapeutic approach to normalize calcium content.
Cancer cells become less deformable and more invasive with activation of β-adrenergic signaling
Authors: Kim Tae-Hyung , Gill Navjot Kaur , Nyberg Kendra D. , Nguyen Angelyn V. , Hohlbauch Sophia V. , Geisse Nicholas A. , Nowell Cameron J. , Sloan Erica K. , Rowat Amy C. 
Published: November 2016

ABSTRACT
Invasion by cancer cells is a crucial step in metastasis. An oversimplified view in the literature is that cancer cells become more deformable as they become more invasive. β-adrenergic receptor (βAR) signaling drives invasion and metastasis, but the effects on cell deformability are not known. Here, we show that activation of β-adrenergic signaling by βAR agonists reduces the deformability of highly metastatic human breast cancer cells, and that these stiffer cells are more invasive in vitro. We find that βAR activation also reduces the deformability of ovarian, prostate, melanoma and leukemia cells. Mechanistically, we show that βAR-mediated cell stiffening depends on the actin cytoskeleton and myosin II activity. These changes in cell deformability can be prevented by pharmacological β-blockade or genetic knockout of the β2-adrenergic receptor. Our results identify a β2-adrenergic–Ca2+–actin axis as a new regulator of cell deformability, and suggest that the relationship between cell mechanical properties and invasion might be dependent on context.
Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy
Authors: Thomé Marcos P. , Filippi-Chiela Eduardo C. , Villodre Emilly S. , Migliavaca Celina B. , Onzi Giovana R. , Felipe Karina B. , Lenz Guido 
Published: November 2016

ABSTRACT
Acridine Orange is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles (AVOs). Its metachromatic shift to red fluorescence is concentration-dependent and, therefore, Acridine Orange fluoresces red in AVOs, such as autolysosomes. This makes Acridine Orange staining a quick, accessible and reliable method to assess the volume of AVOs, which increases upon autophagy induction. Here, we describe a ratiometric analysis of autophagy using Acridine Orange, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of Acridine-Orange-stained cells. This method measured with accuracy the increase in autophagy induced by starvation or rapamycin, and the reduction in autophagy produced by bafilomycin A1 or the knockdown of Beclin1 or ATG7. Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP–LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods.
Free serum haemoglobin is associated with brain atrophy in secondary progressive multiple sclerosis
Authors: Lewin Alex , Hamilton Shea , Witkover Aviva , Langford Paul , Nicholas Richard , Chataway Jeremy , Bangham Charles R.M. 
Published: November 2016

Background: A major cause of disability in secondary progressive multiple sclerosis (SPMS) is progressive brain atrophy, whose pathogenesis is not fully understood. The objective of this study was to identify protein biomarkers of brain atrophy in SPMS. Methods: We used surface-enhanced laser desorption-ionization time-of-flight mass spectrometry to carry out an unbiased search for serum proteins whose concentration correlated with the rate of brain atrophy, measured by serial MRI scans over a 2-year period in a well-characterized cohort of 140 patients with SPMS. Protein species were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Results: There was a significant (p<0.004) correlation between the rate of brain atrophy and a rise in the concentration of proteins at 15.1 kDa and 15.9 kDa in the serum. Tandem mass spectrometry identified these proteins as alpha-haemoglobin and beta-haemoglobin, respectively. The abnormal concentration of free serum haemoglobin was confirmed by ELISA (p<0.001). The serum lactate dehydrogenase activity was also highly significantly raised (p<10-12) in patients with secondary progressive multiple sclerosis. Conclusions: An underlying low-grade chronic intravascular haemolysis is a potential source of the iron whose deposition along blood vessels in multiple sclerosis plaques contributes to the neurodegeneration and consequent brain atrophy seen in progressive disease. Chelators of free serum iron will be ineffective in preventing this neurodegeneration, because the iron (Fe2+) is chelated by haemoglobin.
El proyecto PHI. Patrimonio Histórico+cultural Iberoamericano.
Authors: De Gamón María Estévez , Montoya Joaquín Ibáñez 
Published: November 2016

This is PHI: Ibero-American Cultural and Historic Heritage.The PHI project focuses on developing an innovative global information system to research and study of the cultural legacy of the Hispanic-Portuguese. It is based on two hypotheses: getting the most out of the capabilities of a permanently critical and innovative University world and the design of an instrument with cutting-edge technology to use in our present time.The PHI network: a number of universities coordinated in a homogeneous effort, making use of the present potential of iberoamerican countries, through a collective programming along with local specialized public institutions. This potential can reveal unknown opportunities such as enhance gender equality, improvements in the environment, in city and land planning, and help in the decision-making process. It is a tool of social transformation. A methodology competitive in technology, pursuing improvements in governance, and of focused issues. The PHI platform: an advanced technological system able to manage more efficiently the large inventory of cases collected by academics. As facilitator of teaching innovation, it intends, through the visibility of information, to link researchers, media and ways to do with non-formal and informal social levels of Cultural heritage.
Long‐term exercise in mice has sex‐dependent benefits on body composition and metabolism during aging
Authors: McMullan Rachel C. , Kelly Scott A. , Hua Kunjie , Buckley Brian K. , Faber James E. , Pardo‐Manuel de Villena Fernando , Pomp Daniel 
Published: November 2016

AbstractAging is associated with declining exercise and unhealthy changes in body composition. Exercise ameliorates certain adverse age‐related physiological changes and protects against many chronic diseases. Despite these benefits, willingness to exercise and physiological responses to exercise vary widely, and long‐term exercise and its benefits are difficult and costly to measure in humans. Furthermore, physiological effects of aging in humans are confounded with changes in lifestyle and environment. We used C57BL/6J mice to examine long‐term patterns of exercise during aging and its physiological effects in a well‐controlled environment. One‐year‐old male (n = 30) and female (n = 30) mice were divided into equal size cohorts and aged for an additional year. One cohort was given access to voluntary running wheels while another was denied exercise other than home cage movement. Body mass, composition, and metabolic traits were measured before, throughout, and after 1 year of treatment. Long‐term exercise significantly prevented gains in body mass and body fat, while preventing loss of lean mass. We observed sex‐dependent differences in body mass and composition trajectories during aging. Wheel running (distance, speed, duration) was greater in females than males and declined with age. We conclude that long‐term exercise may serve as a preventive measure against age‐related weight gain and body composition changes, and that mouse inbred strains can be used to characterize effects of long‐term exercise and factors (e.g. sex, age) modulating these effects. These findings will facilitate studies on relationships between exercise and health in aging populations, including genetic predisposition and genotype‐by‐environment interactions.