bluuebunny/crossref_metadata_2025_split huggingface dataset:
Motivating and engaging volunteer hunters to control the invasive alien American mink Neovison vison in Norway
Authors: Stien J. , Hausner V. H. 
Published: January 2017

AbstractInvasive alien species can have widespread negative effects on native biodiversity. We investigated the prospects of engaging hunters in large-scale collaborative efforts to control non-native mink Neovison vison populations in Norway. We invited members of the Norwegian Association of Hunters and Anglers to complete an online questionnaire to ascertain their support for conservation and their level of effort to remove mink, in the context of hunting motivations and bounty payments. The general interest in mink control programmes was low but participants perceived the mink to be of conservation concern. Bounty payments influenced mink catches, with hunters who received payments catching a mean of 4.70 more individuals per hunter than those who did not receive payments (P < 0.001). Mink hunters who preferred to hunt alone and had no preference for hunting locally reportedly caught on average 1.87 more mink per hunter than those who preferred to hunt in company (P < 0.001). The majority of hunters preferred government-led programmes (47%), and investment in bounty programmes (52%) was their main recommendation to improve mink hunting. Hunters can be a valuable component of a mink removal task force and could decrease the likelihood of recolonization of mink in the buffer areas of core conservation target areas.
Chemotherapy of leishmaniasis: present challenges
Authors: ULIANA SILVIA R. B. , TRINCONI CRISTIANA T. , COELHO ADRIANO C. 
Published: January 2017

SUMMARYCutaneous and visceral leishmaniasis are amongst the most devastating infectious diseases of our time, affecting millions of people worldwide. The treatment of these serious diseases rely on a few chemotherapeutic agents, most of which are of parenteral use and induce severe side-effects. Furthermore, rates of treatment failure are high and have been linked to drug resistance in some areas. Here, we reviewed data on current chemotherapy practice in leishmaniasis. Drug resistance and mechanisms of resistance are described as well as the prospects for applying drug combinations for leishmaniasis chemotherapy. It is clear that efforts for discovering new drugs applicable to leishmaniasis chemotherapy are essential. The main aspects on the various steps of drug discovery in the field are discussed.
GANEing traction: The broad applicability of NE hotspots to diverse cognitive and arousal phenomena
Authors: Mather Mara , Clewett David , Sakaki Michiko , Harley Carolyn W. 
Published: January 2017

AbstractThe GANE (glutamate amplifies noradrenergic effects) model proposes that local glutamate–norepinephrine interactions enable “winner-take-more” effects in perception and memory under arousal. A diverse range of commentaries addressed both the nature of this “hotspot” feedback mechanism and its implications in a variety of psychological domains, inspiring exciting avenues for future research.
Bacterial morphotype grading for periodontal disease assessment
Authors: Smeda-Pienaar Kim , Kaambo Eveline , Africa Charlene W J 
Published: January 2017

Abstract
Background:
Listgarten and Hellden (1978) used darkfield microscopy of wet mounts to differentiate between healthy and periodontally diseased sites in the mouth by expressing the different bacterial morphotypes observed as a percentage of the total number of bacteria counted. This method of periodontal disease assessment gained favour as a diagnostic tool but presented with the limitation of immediate examination to determine the number of motile rods present and an inability to distinguish between gingivitis and periodontitis. Grading of bacterial morphotypes into several distinct categories of health or disease (Ison and Hay, 2002), simplified the scoring system of Gram-stained smears for the diagnosis of bacterial vaginosis (Nugent et al. 1991). The application of a similar grading system using stained smears rather than wet mounts could be advantageous to the diagnosis of periodontal disease.

Objectives/aims:
This study tested the hypothesis that stained smears of dental plaque collected from the gingival crevice of individuals with varying probing pocket depths (PD) may provide a grading system for periodontal disease assessment.

Materials and methods:
Subgingival plaque samples were collected from 49 patients, stained with a silver stain and the proportions of each bacterial morphotype graded relative to their respective PD measurements.

Results:
This technique allowed for a grading system of I–IV, with grade I indicating health and grade IV indicating severe periodontal disease.

Discussion:
Stained smear examination eliminates the time restriction for motile rod enumeration and allows for storage of smears for future reference.

Conclusion:
Standardization of the microscopic areas to be evaluated or examined will facilitate the agreement of cut-off values for the diagnosis of periodontal disease.
Exosome-derived microRNAs in cancer metabolism: possible implications in cancer diagnostics and therapy
Authors: Tomasetti Marco , Lee Wan , Santarelli Lory , Neuzil Jiri 
Published: January 2017

AbstractMalignant progression is greatly affected by dynamic cross-talk between stromal and cancer cells. Exosomes are secreted nanovesicles that have key roles in cell–cell communication by transferring nucleic acids and proteins to target cells and tissues. Recently, MicroRNAs (miRs) and their delivery in exosomes have been implicated in physiological and pathological processes. Tumor-delivered miRs, interacting with stromal cells in the tumor microenvironment, modulate tumor progression, angiogenesis, metastasis and immune escape. Altered cell metabolism is one of the hallmarks of cancer. A number of different types of tumor rely on mitochondrial metabolism by triggering adaptive mechanisms to optimize their oxidative phosphorylation in relation to their substrate supply and energy demands. Exogenous exosomes can induce metabolic reprogramming by restoring the respiration of cancer cells and supress tumor growth. The exosomal miRs involved in the modulation of cancer metabolism may be potentially utilized for better diagnostics and therapy.
Improper electric polarization in simple perovskite oxides with two magnetic sublattices
Authors: Zhao Hong Jian , Bellaiche L. , Chen Xiang Ming , Íñiguez Jorge 
Published: January 2017

AbstractABO3 perovskite oxides with magnetic A and B cations offer a unique playground to explore interactions involving two spin sublattices and the emergent effects they may drive. Of particular interest is the possibility of having magnetically driven improper ferroelectricity, as in the much studied families of rare-earth orthoferrites and orthochromites; yet, the mechanisms behind such effects remain to be understood in detail. Here we show that the strongest polar order corresponds to collinear spin configurations and is driven by non-relativistic exchange-strictive mechanisms. Our first-principles simulations reveal the dominant magnetostructural couplings underlying the observed ferroelectricity, including a striking magnetically driven piezoelectric effect. Further, we derive phenomenological and atomistic theories that describe such couplings in a generic perovskite lattice. This allows us to predict how the observed effects can be enhanced, and even how similar ones can be obtained in other perovskite families.
Humans rather than climate the primary cause of Pleistocene megafaunal extinction in Australia
Authors: van der Kaars Sander , Miller Gifford H. , Turney Chris S. M. , Cook Ellyn J. , Nürnberg Dirk , Schönfeld Joachim , Kershaw A. Peter , Lehman Scott J. 
Published: January 2017

AbstractEnvironmental histories that span the last full glacial cycle and are representative of regional change in Australia are scarce, hampering assessment of environmental change preceding and concurrent with human dispersal on the continent ca. 47,000 years ago. Here we present a continuous 150,000-year record offshore south-western Australia and identify the timing of two critical late Pleistocene events: wide-scale ecosystem change and regional megafaunal population collapse. We establish that substantial changes in vegetation and fire regime occurred ∼70,000 years ago under a climate much drier than today. We record high levels of the dung fungus Sporormiella, a proxy for herbivore biomass, from 150,000 to 45,000 years ago, then a marked decline indicating megafaunal population collapse, from 45,000 to 43,100 years ago, placing the extinctions within 4,000 years of human dispersal across Australia. These findings rule out climate change, and implicate humans, as the primary extinction cause.
Anthropogenic ecosystem disturbance and the recovery debt
Authors: Moreno-Mateos David , Barbier Edward B. , Jones Peter C. , Jones Holly P. , Aronson James , López-López José A. , McCrackin Michelle L. , Meli Paula , Montoya Daniel , Rey Benayas José M. 
Published: January 2017

AbstractEcosystem recovery from anthropogenic disturbances, either without human intervention or assisted by ecological restoration, is increasingly occurring worldwide. As ecosystems progress through recovery, it is important to estimate any resulting deficit in biodiversity and functions. Here we use data from 3,035 sampling plots worldwide, to quantify the interim reduction of biodiversity and functions occurring during the recovery process (that is, the ‘recovery debt’). Compared with reference levels, recovering ecosystems run annual deficits of 46–51% for organism abundance, 27–33% for species diversity, 32–42% for carbon cycling and 31–41% for nitrogen cycling. Our results are consistent across biomes but not across degrading factors. Our results suggest that recovering and restored ecosystems have less abundance, diversity and cycling of carbon and nitrogen than ‘undisturbed’ ecosystems, and that even if complete recovery is reached, an interim recovery debt will accumulate. Under such circumstances, increasing the quantity of less-functional ecosystems through ecological restoration and offsetting are inadequate alternatives to ecosystem protection.
The paradoxical functions of EGFR during breast cancer progression
Authors: Ali Remah , Wendt Michael K 
Published: January 2017

AbstractThe epidermal growth factor receptor (EGFR) is one of the most well-studied signaling pathways in cancer progression. As a result, numerous therapeutics including small-molecule inhibitors and monoclonal antibodies have been developed to target this critical oncogenic driver. Several of these EGFR inhibitors (EGFRi) have been evaluated in metastatic breast cancer, as high-level EGFR expression in primary tumors correlates with the highly aggressive basal-like phenotype and predicts for poor patient prognosis. Surprisingly, these trials have been unanimously unsuccessful at improving patient outcomes. Numerous factors, such as lack of proper patient selection may have contributed to the failure of these trials. However, recent findings suggest that there are fundamental changes in EGFR signaling that take place during primary tumor invasion, dissemination and ultimate metastasis of breast cancer cells. Herein, we review the outcomes of EGFR-targeted clinical trials in breast cancer and explore our current understanding of EGFR signaling within primary mammary tumors and how these events are altered in the metastatic setting. Overall, we put forth the hypothesis that fundamental changes in EGFR signaling between primary and metastatic tumors, a process we term the ‘EGFR paradox,’ contribute to the clinically observed inherent resistance to EGFRi. Furthermore, this hypothesis introduces the possibility of utilizing EGFR agonism as a potential therapeutic approach for the treatment of metastatic breast cancer.
Simulating the hydrologic cycle in coal mining subsidence areas with a distributed hydrologic model
Authors: Wang Jianhua , Lu Chuiyu , Sun Qingyan , Xiao Weihua , Cao Guoliang , Li Hui , Yan Lingjia , Zhang Bo 
Published: January 2017

AbstractLarge-scale ground subsidence caused by coal mining and subsequent water-filling leads to serious environmental problems and economic losses, especially in plains with a high phreatic water level. Clarifying the hydrologic cycle in subsidence areas has important practical value for environmental remediation, and provides a scientific basis for water resource development and utilisation of the subsidence areas. Here we present a simulation approach to describe interactions between subsidence area water (SW) and several hydrologic factors from the River-Subsidence-Groundwater Model (RSGM), which is developed based on the distributed hydrologic model. Analysis of water balance shows that the recharge of SW from groundwater only accounts for a small fraction of the total water source, due to weak groundwater flow in the plain. The interaction between SW and groundwater has an obvious annual cycle. The SW basically performs as a net source of groundwater in the wet season, and a net sink for groundwater in the dry season. The results show there is an average 905.34 million m3 per year of water available through the Huainan coal mining subsidence areas (HCMSs). If these subsidence areas can be integrated into water resource planning, the increasingly precarious water supply infrastructure will be strengthened.
Investigation of changes in structure and thermodynamic of spruce budworm antifreeze protein under subfreezing temperature
Authors: Nguyen Hung , Le Ly 
Published: January 2017

AbstractThe aim of this theoretical work is to investigate of the changes in structure and thermodynamics of spruce budworm antifreeze protein (sbAFP) at low temperatures by using molecular dynamics simulation. The aqueous solution will form ice crystal network under the vaguely hexagonal shape at low temperature and fully represented the characteristics of hydrophobic interaction. Like ice crystal network, the cyclohexane region (including cyclohexane molecules) have enough of the characteristics of hydrophobic interaction. Therefore, in this research the cyclohexane region will be used as a representation of ice crystal network to investigate the interactions of sbAFP and ice crystal network at low temperature. The activity of sbAFP in subfreezing environment, therefore, can be clearly observed via the changes of the hydrophobic (cyclohexane region) and hydrophilic (water region) interactions. The obtained results from total energies, hydrogen bond lifetime correlation C(t), radial distribution function, mean square deviation and snapshots of sbAFP complexes indicated that sbAFP has some special changes in structure and interaction with water and cyclohexane regions at 278 K, as being transition temperature point of water molecules in sbAFP complex at low temperatures, which is more structured and support the experimental observation that the sbAFP complex becomes more rigid as the temperature is lowered.
Spectroscopic evidence of odd frequency superconducting order
Authors: Pal Avradeep , Ouassou J. A. , Eschrig M. , Linder J. , Blamire M. G. 
Published: January 2017

AbstractSpin filter superconducting S/I/N tunnel junctions (NbN/GdN/TiN) show a robust and pronounced Zero Bias Conductance Peak (ZBCP) at low temperatures, the magnitude of which is several times the normal state conductance of the junction. Such a conductance anomaly is representative of unconventional superconductivity and is interpreted as a direct signature of an odd frequency superconducting order.
BabA dependent binding of Helicobacter pylori to human gastric mucins cause aggregation that inhibits proliferation and is regulated via ArsS
Authors: Skoog Emma C. , Padra Médea , Åberg Anna , Gideonsson Pär , Obi Ikenna , Quintana-Hayashi Macarena P. , Arnqvist Anna , Lindén Sara K. 
Published: January 2017

AbstractMucins in the gastric mucus layer carry a range of glycan structures, which vary between individuals, can have antimicrobial effect or act as ligands for Helicobacter pylori. Mucins from various individuals and disease states modulate H. pylori proliferation and adhesin gene expression differently. Here we investigate the relationship between adhesin mediated binding, aggregation, proliferation and adhesin gene expression using human gastric mucins and synthetic adhesin ligand conjugates. By combining measurements of optical density, bacterial metabolic activity and live/dead stains, we could distinguish bacterial aggregation from viability changes, enabling elucidation of mechanisms behind the anti-prolific effects that mucins can have. Binding of H. pylori to Leb-glycoconjugates inhibited the proliferation of the bacteria in a BabA dependent manner, similarly to the effect of mucins carrying Leb. Furthermore, deletion of arsS lead to a decrease in binding to Leb-glycoconjugates and Leb-decorated mucins, accompanied by decreased aggregation and absence of anti-prolific effect of mucins and Leb-glycoconjugates. Inhibition of proliferation caused by adhesin dependent binding to mucins, and the subsequent aggregation suggests a new role of mucins in the host defense against H. pylori. This aggregating trait of mucins may be useful to incorporate into the design of adhesin inhibitors and other disease intervention molecules.
Effects of climatic variables on weight loss: a global analysis
Authors: Ustulin Morena , Keum Changwon , Woo Junghoon , Woo Jeong-taek , Rhee Sang Youl 
Published: January 2017

AbstractSeveral studies have analyzed the effects of weather on factors associated with weight loss. In this study, we directly analyzed the effect of weather on intentional weight loss using global-scale data provided by smartphone applications. Through Weather Underground API and the Noom Coach application, we extracted information on weather and body weight for each user located in each of several geographic areas on all login days. We identified meteorological information (pressure, precipitation, wind speed, dew point, and temperature) and self-monitored body weight data simultaneously. A linear mixed-effects model was performed analyzing 3274 subjects. Subjects in North America had higher initial BMIs than those of subjects in Eastern Asia. During the study period, most subjects who used the smartphone application experienced weight loss in a significant way (80.39%, p-value < 0.001). Subjects who infrequently recorded information about dinner had smaller variations than those of other subjects (βfreq.users dinner*time = 0.007, p-value < 0.001). Colder temperature, lower dew point, and higher values for wind speed and precipitation were significantly associated with weight loss. In conclusion, we found a direct and independent impact of meteorological conditions on intentional weight loss efforts on a global scale (not only on a local level).
Asymmetric Waveforms Decrease Lethal Thresholds in High Frequency Irreversible Electroporation Therapies
Authors: Sano Michael B. , Fan Richard E. , Xing Lei 
Published: January 2017

AbstractIrreversible electroporation (IRE) is a promising non-thermal treatment for inoperable tumors which uses short (50–100 μs) high voltage monopolar pulses to disrupt the membranes of cells within a well-defined volume. Challenges with IRE include complex treatment planning and the induction of intense muscle contractions. High frequency IRE (H-FIRE) uses bursts of ultrashort (0.25–5 μs) alternating polarity pulses to produce more predictable ablations and alleviate muscle contractions associated with IRE. However, H-FIRE generally ablates smaller volumes of tissue than IRE. This study shows that asymmetric H-FIRE waveforms can be used to create ablation volumes equivalent to standard IRE treatments. Lethal thresholds (LT) of 505 V/cm and 1316 V/cm were found for brain cancer cells when 100 μs IRE and 2 μs symmetric H-FIRE waveforms were used. In contrast, LT as low as 536 V/cm were found for 2 μs asymmetric H-FIRE waveforms. Reversible electroporation thresholds were 54% lower than LTs for symmetric waveforms and 33% lower for asymmetric waveforms indicating that waveform symmetry can be used to tune the relative sizes of reversible and irreversible ablation zones. Numerical simulations predicted that asymmetric H-FIRE waveforms are capable of producing ablation volumes which were 5.8–6.3x larger than symmetric H-FIRE waveforms indicating that in vivo investigation of asymmetric waveforms is warranted.
Identification of novel cellular clusters define a specialized area in the cerebellar periventricular zone
Authors: González-González María Alejandra , Gómez-González Gabriela B. , Becerra-González Marymar , Martínez-Torres Ataúlfo 
Published: January 2017

AbstractThe periventricular zone of cerebellum is a germinative niche during the embryonic development, nevertheless its structural organization and functional implications in adult have not been widely studied. Here we disclose the presence of two novel clusters of cells in that area. The first one was named the subventricular cellular cluster (SVCC) and is composed of cells that express glial and neuronal markers. The second was named the ventromedial cord (VMC) and appears as a streak of biciliated cells with microvillosities facing the ventricle, that includes GFAP+ and nestin+ cells organized along the periventricular vasculature. The dorsal limit of the SVCC is associated with myelinated axons of neurons of unknown origin. This paper describes the characteristics and organization of these groups of cells. They can be observed from late embryonic development in the transgenic mouse line GFAP-GFP. The SVCC and VMC expand during early postnatal development but are restricted to the central area of the ventricle in adulthood. We did not find evidence of cell proliferation, cell migration or the presence of fenestrated blood vessels. These findings provide new insights into the knowledge of the cellular composition and structural organization of the periventricular zone of cerebellum.
Modification of tumour cell metabolism modulates sensitivity to Chk1 inhibitor-induced DNA damage
Authors: Massey Andrew J. 
Published: January 2017

AbstractChk1 kinase inhibitors are currently under clinical investigation as potentiators of cytotoxic chemotherapy and demonstrate potent activity in combination with anti-metabolite drugs that increase replication stress through the inhibition of nucleotide or deoxyribonucleotide biosynthesis. Inhibiting other metabolic pathways critical for the supply of building blocks necessary to support DNA replication may lead to increased DNA damage and synergy with an inhibitor of Chk1. A screen of small molecule metabolism modulators identified combinatorial activity between a Chk1 inhibitor and chloroquine or the LDHA/LDHB inhibitor GSK 2837808A. Compounds, such as 2-deoxyglucose or 6-aminonicotinamide, that reduced the fraction of cells undergoing active replication rendered tumour cells more resistant to Chk1 inhibitor-induced DNA damage. Withdrawal of glucose or glutamine induced G1 and G2/M arrest without increasing DNA damage and reduced Chk1 expression and activation through autophosphorylation. This suggests the expression and activation of Chk1 kinase is associated with cells undergoing active DNA replication. Glutamine starvation rendered tumour cells more resistant to Chk1 inhibitor-induced DNA damage and reversal of the glutamine starvation restored the sensitivity of tumour cells to Chk1 inhibitor-induced DNA damage. Chk1 inhibitors may be a potentially useful therapeutic treatment for patients whose tumours contain a high fraction of replicating cells.