bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors Affecting Anti-Glycan IgG and IgM Repertoires in Human Serum
Authors: Muthana Saddam M. , Gildersleeve Jeffrey C. 
Published: January 2016

AbstractSerum anti-glycan antibodies play important roles in many immune processes and are of particular interest as biomarkers for many diseases. Changes in anti-glycan antibodies can occur with the onset of disease or in response to stimuli such as pathogens and vaccination. Understanding relationships between anti-glycan antibody repertoires and genetic and environment factors is critical for basic research and clinical applications, but little information is available. In this study we evaluated the effects of age, race, gender and blood type on anti-glycan antibody profiles in the serum of 135 healthy subjects. As expected, IgG and IgM antibody signals to blood group antigens correlated strongly with blood type. Interestingly, antibodies to other non-ABH glycans, such as the alpha-Gal antigen, also correlated with blood type. A statistically significant decline in IgM signals with age was observed for many antibody subpopulations, but not for IgG. Moreover, statistically significant correlations between race and IgG levels to certain LacNAc-containing glycans were observed. The results have important implications for designing studies and interpreting results in the area of biomarker discovery and for the development of vaccines. The study also highlights the importance of collecting and reporting patient information that could affect serum anti-glycan antibody levels.
Preparation of Pd supported on La(Sr)-Mn-O Perovskite by microwave Irradiation Method and Its Catalytic Performances for the Methane Combustion
Authors: Wang Wei , Yuan Fulong , Niu Xiaoyu , Zhu Yujun 
Published: January 2016

AbstractIn this work, a series of palladium supported on the La0.8Sr0.2MnO3.15 perovskite catalysts (Pd/LSM-x) with different Pd loading were prepared by microwave irradiation processing plus incipient wetness impregnation method and characterized by XRD, TEM, H2-TPR and XPS. These catalysts were evaluated on the lean CH4 combustion. The results show that the Pd/LSM-x samples prepared by microwave irradiation processing possess relative higher surface areas than LSM catalyst. The addition of Pd to the LSM leads to the increase in the oxygen vacancy content and the enhancement in the mobility of lattice oxygen which play an important role on the methane combustion. The Pd/LSM-3 catalysts with 4.2wt% Pd loading exhibited the best performance for CH4 combustion that temperature for 10% and 90% of CH4 conversion is 315 and 520 °C.
Perfect and broadband acoustic absorption by critically coupled sub-wavelength resonators
Authors: Romero-García V. , Theocharis G. , Richoux O. , Merkel A. , Tournat V. , Pagneux V. 
Published: January 2016

AbstractPerfect absorption is an interdisciplinary topic with a large number of applications, the challenge of which consists of broadening its inherently narrow frequency-band performance. We experimentally and analytically report perfect and broadband absorption for audible sound, by the mechanism of critical coupling, with a sub-wavelength multi-resonant scatterer (SMRS) made of a plate-resonator/closed waveguide structure. In order to introduce the role of the key parameters, we first present the case of a single resonant scatterer (SRS) made of a Helmholtz resonator/closed waveguide structure. In both cases the controlled balance between the energy leakage of the several resonances and the inherent losses of the system leads to perfect absorption peaks. In the case of the SMRS we show that systems with large inherent losses can be critically coupled using resonances with large leakage. In particular, we show that in the SMRS system, with a thickness of λ/12 and diameter of λ/7, several perfect absorption peaks overlap to produce absorption bigger than 93% for frequencies that extend over a factor of 2 in audible frequencies. The reported concepts and methodology provide guidelines for the design of broadband perfect absorbers which could contribute to solve the major issue of noise reduction.
The Epstein-Barr virus encoded LMP1 oncoprotein modulates cell adhesion via regulation of activin A/TGFβ and β1 integrin signalling
Authors: Morris Mhairi A. , Dawson Christopher W. , Laverick Louise , Davis Alexandra M. , Dudman Joe P. R. , Raveenthiraraj Sathuwarman , Ahmad Zeeshan , Yap Lee-Fah , Young Lawrence S. 
Published: January 2016

AbstractApproximately 20% of global cancer incidence is causally linked to an infectious agent. Epstein-Barr virus (EBV) accounts for around 1% of all virus-associated cancers and is associated with nasopharyngeal carcinoma (NPC). Latent membrane protein 1 (LMP1), the major oncoprotein encoded by EBV, behaves as a constitutively active tumour necrosis factor (TNF) receptor activating a variety of signalling pathways, including the three classic MAPKs (ERK-MAPK, p38 MAPK and JNK/SAPK). The present study identifies novel signalling properties for this integral membrane protein via the induction and secretion of activin A and TGFβ1, which are both required for LMP1’s ability to induce the expression of the extracellular matrix protein, fibronectin. However, it is evident that LMP1 is unable to activate the classic Smad-dependent TGFβ signalling pathway, but rather elicits its effects through the non-Smad arm of TGFβ signalling. In addition, there is a requirement for JNK/SAPK signalling in LMP1-mediated fibronectin induction. LMP1 also induces the expression and activation of the major fibronectin receptor, α5β1 integrin, an effect that is accompanied by increased focal adhesion formation and turnover. Taken together, these findings support the putative role for LMP1 in the pathogenesis of NPC by contributing to the metastatic potential of epithelial cells.
Rspo1-activated signalling molecules are sufficient to induce ovarian differentiation in XY medaka (Oryzias latipes)
Authors: Zhou Linyan , Charkraborty Tapas , Zhou Qian , Mohapatra Sipra , Nagahama Yoshitaka , Zhang Yueguang 
Published: January 2016

AbstractIn contrast to our understanding of testicular differentiation, ovarian differentiation is less well understood in vertebrates. In mammals, R-spondin1 (Rspo1), an activator of Wnt/β-catenin signaling pathway, is located upstream of the female sex determination pathway. However, the functions of Rspo1 in ovarian differentiation remain unclear in non-mammalian species. In order to elucidate the detailed functions of Rspo/Wnt signaling pathway in fish sex determination/differentiation, the ectopic expression of the Rspo1 gene was performed in XY medaka (Oryzias latipes). The results obtained demonstrated that the gain of Rspo1 function induced femininity in XY fish. The overexpression of Rspo1 enhanced Wnt4b and β-catenin transcription and completely suppressed the expression of male-biased genes (Dmy, Gsdf, Sox9a2 and Dmrt1) as well as testicular differentiation. Gonadal reprograming of Rspo1-over-expressed-XY (Rspo1-OV-XY) fish, induced the production of female-biased genes (Cyp19a1a and Foxl2), estradiol-17β production and further female type secondary sexuality. Moreover, Rspo1-OV-XY females were fertile and produced successive generations. Promoter analyses showed that Rspo1 transcription was directly regulated by DM domain genes (Dmy, the sex-determining gene and Dmrt1) and remained unresponsive to Foxl2. Taken together, our results strongly suggest that Rspo1 is sufficient to activate ovarian development and plays a decisive role in the ovarian differentiation in medaka.
Temperature stable mid-infrared GaInAsSb/GaSb Vertical Cavity Surface Emitting Lasers (VCSELs)
Authors: Ikyo A. B. , Marko I. P. , Hild K. , Adams A. R. , Arafin S. , Amann M.-C. , Sweeney S. J. 
Published: January 2016

AbstractGaInAsSb/GaSb based quantum well vertical cavity surface emitting lasers (VCSELs) operating in mid-infrared spectral range between 2 and 3 micrometres are of great importance for low cost gas monitoring applications. This paper discusses the efficiency and temperature sensitivity of the VCSELs emitting at 2.6 μm and the processes that must be controlled to provide temperature stable operation. We show that non-radiative Auger recombination dominates the threshold current and limits the device performance at room temperature. Critically, we demonstrate that the combined influence of non-radiative recombination and gain peak – cavity mode de-tuning determines the overall temperature sensitivity of the VCSELs. The results show that improved temperature stable operation around room temperature can only be achieved with a larger gain peak – cavity mode de-tuning, offsetting the significant effect of increasing non-radiative recombination with increasing temperature, a physical effect which must be accounted for in mid-infrared VCSEL design.
Molecular Combing of Single DNA Molecules on the 10 Megabase Scale
Authors: Kaykov Atanas , Taillefumier Thibaud , Bensimon Aaron , Nurse Paul 
Published: January 2016

AbstractDNA combing allows the investigation of DNA replication on genomic single DNA molecules, but the lengths that can be analysed have been restricted to molecules of 200–500 kb. We have improved the DNA combing procedure so that DNA molecules can be analysed up to the length of entire chromosomes in fission yeast and up to 12 Mb fragments in human cells. Combing multi-Mb-scale DNA molecules revealed previously undetected origin clusters in fission yeast and shows that in human cells replication origins fire stochastically forming clusters of fired origins with an average size of 370 kb. We estimate that a single human cell forms around 3200 clusters at mid S-phase and fires approximately 100,000 origins to complete genome duplication. The procedure presented here will be adaptable to other organisms and experimental conditions.
Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators
Authors: Wei Shu , Zou Qingjian , Lai Sisi , Zhang Quanjun , Li Li , Yan Quanmei , Zhou Xiaoqing , Zhong Huilin , Lai Liangxue 
Published: January 2016

AbstractThe recently emerged CRISPR/Cas9 technique has opened a new perspective on readily editing specific genes. When combined with transcription activators, it can precisely manipulate endogenous gene expression. Here, we enhanced the expression of endogenous Cdx2 and Gata6 genes by CRISPR-mediated activators, thus mouse embryonic stem cells (ESCs) were directly converted into two extraembryonic lineages, i.e., typical trophoblast stem cells (TSCs) and extraembryonic endoderm cells (XENCs), which exhibited characters of TSC or XENC derived from the blastocyst extraembryonic lineages such as cell morphology, specific gene expression and differentiation ability in vitro and in vivo. This study demonstrates that the cell fate can be effectively manipulated by directly activating of specific endogenous gene expression with CRISPR-mediated activator.
Diffusion magnetic resonance imaging study of schizophrenia in the context of abnormal neurodevelopment using multiple site data in a Chinese Han population
Authors: Li Y , Xie S , Liu B , Song M , Chen Y , Li P , Lu L , Lv L , Wang H , Yan H , Yan J , Zhang H , Zhang D , Jiang T 
Published: January 2016

AbstractSchizophrenia has increasingly been considered a neurodevelopmental disorder, and the advancement of neuroimaging techniques and associated computational methods has enabled quantitative re-examination of this important theory on the pathogenesis of the disease. Inspired by previous findings from neonatal brains, we proposed that an increase in diffusion magnetic resonance imaging (dMRI) mean diffusivity (MD) should be observed in the cerebral cortex of schizophrenia patients compared with healthy controls, corresponding to lower tissue complexity and potentially a failure to reach cortical maturation. We tested this hypothesis using dMRI data from a Chinese Han population comprising patients from four different hospital sites. Utilizing data-driven methods based on the state-of-the-art tensor-based registration algorithm, significantly increased MD measurements were consistently observed in the cortex of schizophrenia patients across all four sites, despite differences in psychopathology, exposure to antipsychotic medication and scanners used for image acquisition. Specifically, we found increased MD in the limbic system of the schizophrenic brain, mainly involving the bilateral insular and prefrontal cortices. In light of the existing literature, we speculate that this may represent a neuroanatomical signature of the disorder, reflecting microstructural deficits due to developmental abnormalities. Our findings not only provide strong support to the abnormal neurodevelopment theory of schizophrenia, but also highlight an important neuroimaging endophenotype for monitoring the developmental trajectory of high-risk subjects of the disease, thereby facilitating early detection and prevention.
Conduct disorder in females is associated with reduced corpus callosum structural integrity independent of comorbid disorders and exposure to maltreatment
Authors: Lindner P , Savic I , Sitnikov R , Budhiraja M , Liu Y , Jokinen J , Tiihonen J , Hodgins S 
Published: January 2016

AbstractThe behavioral phenotype and genotype of conduct disorder (CD) differ in males and females. Abnormalities of white matter integrity have been reported among males with CD and antisocial personality disorder (ASPD). Little is known about white matter integrity in females with CD. The present study aimed to determine whether abnormalities of white matter are present among young women who presented CD before the age of 15, and whether abnormalities are independent of the multiple comorbid disorders and experiences of maltreatment characterizing females with CD that may each in themselves be associated with alterations of the white matter. Three groups of women, aged on average 24 years, were scanned using diffusion tensor imaging and compared: 28 with prior CD, three of whom presented ASPD; a clinical comparison (CC) group of 15 women with no history of CD but with similar proportions who presented alcohol dependence, drug dependence, anxiety disorders, depression disorders and physical and sexual abuse as the CD group; and 24 healthy women. Whole-brain, tract-based spatial statistics were computed to investigate differences in fractional anisotropy, axial diffusivity and radial diffusivity. Compared with healthy women, women with prior CD showed widespread reductions in axial diffusivity primarily in frontotemporal regions. After statistically adjusting for comorbid disorders and maltreatment, group differences in the corpus callosum body and genu (including forceps minor) remained significant. Compared with the CC group, women with CD showed reduced fractional anisotropy in the body and genu of the corpus callosum. No differences were detected between the CD and healthy women in the uncinate fasciculus.
“Confusion is a fundamental state of mind”—On the peculiar intellectual career of global governance in international relations
Authors: Hofferberth Matthias 
Published: January 2016

AbstractMore than 20 years after global governance was introduced to the discipline of International Relations (IR), confusion about its conceptual status remains. In fact, how to even speak and think global governance—whether as a description of world politics, as a theoretical perspective to explain it, or as a normative notion to be realized through global policy—remains debated. This state of confusion affects debates within the Political Science subfield of IR as well as dialogue between different disciplines beyond IR. More specifically, the article argues that the current state of confusion exists not because of a lack of debate but rather because of different understandings of global governance that were attached to the concept during its emergence and which are still advanced and reproduced within debates today. These different understandings have their origin in certain real-world and disciplinary dynamics and constitute global governance in present discourse as a “condensation symbol” of different meanings. It is argued that precisely because of this status, global governance has obtained its “celebrity status”, within and beyond IR. By structuring different understandings of global governance and by reconstructing real-world and disciplinary contexts of emergence, the article goes beyond stating the obvious and provides a discussion of various sources and consequences of the confusion surrounding global governance. On the basis of this state-of-the-art overview, it is argued that to realize the full potential of global governance in IR and ensure interdisciplinary dialogue beyond it, one needs to engage with the concept and its immanent confusion in a reflective and cautious way by becoming aware of different meanings attached to it instead of arbitrarily reducing the concept to a single meaning to define its conceptual status. This article is published as part of a thematic collection on global governance.
Global governance: present and future
Authors: Jang Jinseop , McSparren Jason , Rashchupkina Yuliya 
Published: January 2016

AbstractGlobalization, the end of the Cold War and increased involvement of non-state actors in global affairs represent fundamentally shifting relations of power, speeding up national economies’ integration and contributing to the convergence of policies in different issue domains. This review considers the state of global governance by presenting a variety of global governance arrangements, key challenges facing governance in an increasingly globalized context and possibilities for the future governance. Current global governance arrangements favour flexibility over rigidity, prefer voluntary measures to binding rules and privilege partnerships over individual actions. This synopsis of the state of global governance examines the evolving role that sovereignty and the enduring human struggles for power and equity are playing in shaping international relations and governance. This contribution argues that individual empowerment, increasing awareness of human security, institutional complexity, international power shifts and the liberal world political paradigm will define the future of global governance. This article is published as part of a thematic collection dedicated to global governance.
Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551
Authors: Nakayama Kosuke , Ohmori Takeshi , Ishikawa Satoshi , Iwata Natsumi , Seto Yasuo , Kawahara Kazuyoshi 
Published: January 2016

Abstract
The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.
Three‐dimensional kinematics of competitive and recreational cyclists across different workloads during cycling
Authors: Bini Rodrigo R. , Dagnese Frederico , Rocha Emmanuel , Silveira Mateus C. , Carpes Felipe P. , Mota Carlos B. 
Published: January 2016

AbstractAlthough the link between sagittal plane motion and exercise intensity has been highlighted, no study assessed if different workloads lead to changes in three‐dimensional cycling kinematics. This study compared three‐dimensional joint and segment kinematics between competitive and recreational road cyclists across different workloads. Twenty‐four road male cyclists (12 competitive and 12 recreational) underwent an incremental workload test to determine aerobic peak power output. In a following session, cyclists performed four trials at sub‐maximal workloads (65, 75, 85 and 95% of their aerobic peak power output) at 90 rpm of pedalling cadence. Mean hip adduction, thigh rotation, shank rotation, pelvis inclination (latero‐lateral and anterior–posterior), spine inclination and rotation were computed at the power section of the crank cycle (12 o'clock to 6 o'clock crank positions) using three‐dimensional kinematics. Greater lateral spine inclination (p < .01, 5–16%, effect sizes = 0.09–0.25) and larger spine rotation (p < .01, 16–29%, effect sizes = 0.31–0.70) were observed for recreational cyclists than competitive cyclists across workload trials. No differences in segment and joint angles were observed from changes in workload with significant individual effects on spine inclination (p < .01). No workload effects were found in segment angles but differences, although small, existed when comparing competitive road to recreational cyclists. When conducting assessment of joint and segment motions, workload between 65 and 95% of individual cyclists’ peak power output could be used.
Understanding the consequences of victory amongst sport spectators: The mediating role of BIRGing
Authors: Bernache‐Assollant Iouri , Chantal Yves , Bouchet Patrick , Lacassagne Marie‐Françoise 
Published: January 2016

AbstractPrior work on the relationships between team identification and spectators’ reactions to one's team victory has largely neglected the potential effects of mediating variables. In this research, we proposed that the process of Basking in Reflected Glory [BIRGing – the tendency to reduce the distance between oneself and one's team; Cialdini, R. B., Bordon, R. J., Thorne, A., Walker, M. R., Freeman, S., & Sloan, L. R. (1976). Basking in reflected glory: Three (football) field studies. Journal of Personality and Social Psychology, 34, 366–375] would lead spectators to almost take ownership of victories and share its benefits, thereby impacting their sense of national belonging in a positive manner. To this end, participants (N = 73) were asked to watch an edited video clip showing a victory of the French national rugby team playing against South Africa. As expected, results revealed that BIRGing mediated the relationship between team identification and one's sense of national belonging. In closing, a number of implications are discussed along with future research avenues pertaining to the sport spectatorship literature.
The physical impact of towed demersal fishing gears on soft sediments
Authors: O'Neill F. G. , Ivanović A. 
Published: January 2016

Abstract
An improved understanding of the physical interaction of towed demersal fishing gears with the seabed has been developed in recent years, and there is a clearer view of the underpinning mechanical processes that lead to the modification and alteration of the benthic environment. The physical impact of these gears on soft sediments can be classified broadly as being either geotechnical or hydrodynamic in nature: penetration and piercing of the substrate, lateral displacement of sediment, and the influence of the pressure field transmitted through the sediment can be considered geotechnical, whereas the mobilization of sediment into the water column can be considered hydrodynamic. A number of experimental and numerical approaches have been used to gain better insights of these physical processes. These include small-scale modelling in towing tanks and sand channels; large-scale modelling in the field; measurements behind full-scale towed gears at sea; numerical/mathematical modelling of sediment mechanics; and numerical/mathematical modelling of hydrodynamics. Here, we will review this research, and that in associated fields, and show how it can form the basis of predictive models of the benthic impact of trawl gears.