bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Variants in the Transcriptional Regulatory Region of the ALOX5AP gene and Susceptibility to Ischemic Stroke in Chinese Populations
Authors: Yang Dongzhi , Huang Xiangnan , Cui Chuanju , Zhang Yuchao , Li Ya , Zang Xin , He Ying , Zheng Hong 
Published: July 2016

AbstractNo coding sequence variants of the ALOX5AP gene that lead to amino acid substitutions have been identified. A two-stage study design was used to explore the relationship between variants in the transcriptional regulatory region of ALOX5AP gene and ischemic stroke (IS) risk in Chinese populations. IS was determined using CT and/or MRI. First, 18 SNPs, located in the upstream promoter region of ALOX5AP gene, were genotyped in 200 IS patients and 200 controls. And one potential associated SNP (rs17222919) was identified (P = 0.005,OR = 0.623, 95% CI: 0.448~0.866). Next, another independent case-control cohort comprising 810 IS patients and 825 matched controls was recruited to investigate the role of rs17222919, rs9579646 polymorphisms and their haplotypes in IS risk. The G allele frequency of rs17222919 in the IS group was significantly lower than that in control group (P = 0.007, OR = 0.792, 95% CI: 0.669~0.937). T-A and G-A haplotypes were associated with IS (P = 0.001,OR = 1.282, 95% CI:1.100~1.495; P = 0.0001, OR = 0.712, 95% CI: 0.598~0.848; respectively). Our study providesevidence that rs17222919 is a potential genetic protective factor against IS. Furthermore, the T-A haplotype is a risk factor and the G-A haplotype is a protective factor against IS in Chinese population.
Reflection type metasurface designed for high efficiency vectorial field generation
Authors: Wang Shiyi , Zhan Qiwen 
Published: July 2016

AbstractWe propose a reflection type metal-insulator-metal (MIM) metasurface composed of hybrid nano-antennas for comprehensive spatial engineering of the properties of optical fields. The capability of such structure is illustrated in the design of a device that can be used to produce a radially polarized vectorial beam for optical needle field generation. This device consists of uniformly segmented sectors of high efficiency MIM metasurface. With each of the segment sector functioning as a local quarter-wave-plate (QWP), the device is designed to convert circularly polarized incidence into local linear polarization to create an overall radial polarization with corresponding binary phases and extremely high dynamic range amplitude modulation. The capability of such devices enables the generation of nearly arbitrarily complex optical fields that may find broad applications that transcend disciplinary boundaries.
Knock out of the PHOSPHATE 2 Gene TaPHO2-A1 Improves Phosphorus Uptake and Grain Yield under Low Phosphorus Conditions in Common Wheat
Authors: Ouyang Xiang , Hong Xia , Zhao Xueqiang , Zhang Wei , He Xue , Ma Wenying , Teng Wan , Tong Yiping 
Published: July 2016

AbstractMiR399 and its targetPHOSPHATE2 (PHO2) play pivotal roles in phosphate signaling in plants. Loss of function mutation inPHO2leads to excessive Pi accumulation in shoots and growth retardation in diploid plants likeArabidopsis thalianaand rice (Oryza sativa). Here we isolated threePHO2homologous genesTaPHO2-A1, -B1and -D1from hexaploid wheat (Triticum aestivum). TheseTaPHO2genes all contained miR399-binding sites and were able to be degraded by tae-miR399.TaPHO2-D1was expressed much more abundantly thanTaPHO2-A1and -B1. The ion beam-induced deletion mutants were used to analyze the effects ofTaPHO2son phosphorus uptake and plant growth. Thetapho2-a1, tapho2-b1andtapho2-d1mutants all had significant higher leaf Pi concentrations than did the wild type, withtapho2-d1having the strongest effect andtapho2-b1the weakest. Two consecutive field experiments showed that knocking outTaPHO2-D1reduced plant height and grain yield under both low and high phosphorus conditions. However, knocking outTaPHO2-A1significantly increased phosphorus uptake and grain yield under low phosphorus conditions, with no adverse effect on grain yield under high phosphorus conditions. Our results indicated thatTaPHO2sinvolved in phosphorus uptake and translocation and molecular engineeringTaPHO2shows potential in improving wheat yield with less phosphorus fertilizer.
Indole-3-carbinol protects against cisplatin-induced acute nephrotoxicity: role of calcitonin gene-related peptide and insulin-like growth factor-1
Authors: El-Naga Reem N. , Mahran Yasmen F. 
Published: July 2016

AbstractNephrotoxicity associated with the clinical use of the anticancer drug cisplatin is a limiting problem. Thus, searching for new protective measures is required. Indole-3-carbinol is a powerful anti-oxidant, anti-inflammatory and anti-tumor agent. The present study aimed to investigate the potential protective effect of indole-3-carbinol against cisplatin-induced acute nephrotoxicity in rats. Rats were pre-treated with 20 mg/kg indole-3-carbinol orally before giving cisplatin (7 mg/kg). Cisplatin-induced acute nephrotoxicity was demonstrated where relative kidney weight, BUN and serum creatinine were significantly increased. Increased oxidative stress was evident in cisplatin group where GSH and SOD tissue levels were significantly depleted. Also, lipid peroxidation and NOX-1 were increased as compared to the control. Additionally, renal expression of pro-inflammatory mediators was induced by cisplatin. Cisplatin-induced cell death was shown by increased caspase-3 and decreased expression of EGF, IGF-1 and IGF-1 receptor. Nephrotoxicity, oxidative stress, inflammation and apoptotic effects induced by cisplatin were significantly ameliorated by indole-3-carbinol pre-treatment. Besides, the role of CGRP in cisplatin-induced nephrotoxicity was explored. Furthermore, cisplatin cytotoxic activity was significantly enhanced by indole-3-carbinol pre-treatment in vitro. In conclusion, indole-3-carbinol provides protection against cisplatin-induced nephrotoxicity. Also, reduced expression of CGRP may play a role in the pathogenesis of cisplatin-induced renal injury.
Weight Gain in Infancy and Overweight or Obesity in Childhood across the Gestational Spectrum: a Prospective Birth Cohort Study
Authors: Wang Guoying , Johnson Sara , Gong Yiwei , Polk Sarah , Divall Sara , Radovick Sally , Moon Margaret , Paige David , Hong Xiumei , Caruso Deanna , Chen Zhu , Mallow Eric , Walker Sheila O. , Mao Guangyun , Pearson Colleen , Wang Mei-Cheng , Zuckerman Barry , Cheng Tina L. , Wang Xiaobin 
Published: July 2016

AbstractThis study aimed to investigate the optimal degree of weight gain across the gestational spectrum in 1971 children enrolled at birth and followed up to age 7 years. Weight gain in infancy was categorized into four groups based on weight gain z-scores: slow (<−0.67), on track (−0.67 to 0.67), rapid (0.67 to 1.28), and extremely rapid (>1.28). Underweight and overweight or obesity (OWO) were defined as a body mass index ≤5th and ≥85th percentile, respectively, for age and gender. In our population, OWO was far more common than underweight (39.7% vs. 3.6%). Weight gain tracked strongly from age 4 to 24 months, and was positively associated with OWO and an unfavorable pattern of metabolic biomarkers, although the degree of weight gain for the risk was different across gestational categories. Extremely rapid weight gain led to a particularly high risk of OWO among children born early term and late preterm: odds ratio: 3.3 (95% confidence interval: 1.9 to 5.5) and 3.7 (1.8 to 7.5), respectively, as compared to those with on track weight gain. Our findings suggest that monitoring and ensuring optimal weight gain across the entire gestational spectrum beginning from birth represents a first step towards primary prevention of childhood obesity.
Copy number variation of E3 ubiquitin ligase genes in peripheral blood leukocyte and colorectal cancer
Authors: Bi Haoran , Tian Tian , Zhu Lin , Zhou Haibo , Hu Hanqing , Liu Yanhong , Li Xia , Hu Fulan , Zhao Yashuang , Wang Guiyu 
Published: July 2016

AbstractGiven that E3 ubiquitin ligases (E3) regulate specific protein degradation in many cancer-related biological processes. E3 copy number variation (CNV) may affect the development and prognosis of colorectal cancer (CRC). Therefore, we detected CNVs of five E3 genes in 518 CRC patients and 518 age, gender and residence matched controls in China and estimated the association between E3 gene CNVs and CRC risk and prognosis. We also estimated their interactions with environmental factors and CRC risk. We find a significant association between the CNVs of MDM2 and CRC risk (amp v.s. wt: odds ratio = 14.37, 95% confidence interval: 1.27, 163.74, P = 0.032), while SKP2 CNVs may significantly decrease CRC risk (del v.s. wt: odds ratio = 0.32, 95% confidence interval: 0.10, 1.00, P = 0.050). However, we find no significant association between the CNVs of other genes and CRC risk. The only significant gene-environment interaction effects are between SKP2 CNVs and consumption of fish and/or fruit (P = 0.014 and P = 0.035) and between FBXW7 CNVs and pork intake (P = 0.040). Finally, we find marginally significant association between β-TRCP CNVs and CRC prognosis (amp v.s. wt, hazard ratio = 0.42, 95% confidence interval: 0.19, 0.97, P = 0.050).
Hollow cathode lamp based Faraday anomalous dispersion optical filter
Authors: Pan Duo , Xue Xiaobo , Shang Haosen , Luo Bin , Chen Jingbiao , Guo Hong 
Published: July 2016

AbstractThe Faraday anomalous dispersion optical filter (FADOF), which has acquired wide applications, is mainly limited to some gaseous elements and low melting-point metals before, for the restriction of the attainable atomic density. In conventional FADOF systems a high atomic density is usually achieved by thermal equilibrium at the saturated vapor pressure, hence for elements with high melting-points a high temperature is required. To avoid this restriction, we propose a scheme of FADOF based on the hollow cathode lamp (HCL), instead of atomic vapor cells. Experimental results in strontium atoms verified this scheme, where a transmission peak corresponding to the 88Sr (5s2)1S0 − (5s5p)1P1 transition (461 nm) is obtained, with a maximum transmittance of 62.5% and a bandwith of 1.19 GHz. The dependence of transmission on magnetic field and HCL discharge current is also studied. Since the state-of-art commercial HCLs cover about 70 elements, this scheme can greatly expand the applications of FADOFs and the abundant atomic transitions they provide bring the HCL based FADOFs potential applications for frequency stabilization.
Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway
Authors: Ma Ting , Fan Bo-Yi , Zhang Chao , Zhao Hui-Jun , Han Chao , Gao Cai-Yun , Luo Jian-Guang , Kong Ling-Yi 
Published: July 2016

AbstractMetabolomics can be used to identify potential markers and discover new targets for future therapeutic interventions. Here, we developed a novel application of the metabonomics method based on gas chromatography-mass spectrometry (GC/MS) analysis and principal component analysis (PCA) for rapidly exploring the anticancer mechanism of physapubenolide (PB), a cytotoxic withanolide isolated from Physalis species. PB inhibited the proliferation of hepatocellular carcinoma cells in vitro and in vivo, accompanied by apoptosis-related biochemical events, including the cleavage of caspase-3/7/9 and PARP. Metabolic profiling analysis revealed that PB disturbed the metabolic pattern and significantly decreased lactate production. This suggests that the suppression of glycolysis plays an important role in the anti-tumour effects induced by PB, which is further supported by the decreased expression of glycolysis-related genes and proteins. Furthermore, the increased level of p53 and decreased expression of p-Akt were observed and the attenuated glycolysis and enhanced apoptosis were reversed in the presence of Akt cDNA or p53 siRNA. These results confirm that PB exhibits anti-cancer activities through the Akt-p53 pathway. Our study not only reports for the first time the anti-tumour mechanism of PB, but also suggests that PB is a promising therapeutic agent for use in cancer treatments and that metabolomic approaches provide a new strategy to effectively explore the molecular mechanisms of promising anticancer compounds.
Mitochondrial cereblon functions as a Lon-type protease
Authors: Kataoka Kosuke , Nakamura China , Asahi Toru , Sawamura Naoya 
Published: July 2016

AbstractLon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. However, whether CRBN has Lon protease-like function remains unknown. Here, we determined if CRBN has a protective function against oxidative stress, similar to Lon protease. We report that CRBN partially distributes in mitochondria, suggesting it has a mitochondrial function. To specify the mitochondrial role of CRBN, we mitochondrially expressed CRBN in human neuroblastoma SH-SY5Y cells. The resulting stable SH-SY5Y cell line showed no apparent effect on the mitochondrial functions of fusion, fission and membrane potential. However, mitochondrially expressed CRBN exhibited protease activity and was induced by oxidative stress. In addition, stably expressed cells exhibited suppressed neuronal cell death induced by hydrogen peroxide. These results suggest that CRBN functions specifically as a Lon-type protease in mitochondria.
Four-state memory based on a giant and non-volatile converse magnetoelectric effect in FeAl/PIN-PMN-PT structure
Authors: Wei Yanping , Gao Cunxu , Chen Zhendong , Xi Shibo , Shao Weixia , Zhang Peng , Chen Guilin , Li Jiangong 
Published: July 2016

AbstractWe report a stable, tunable and non-volatile converse magnetoelectric effect (ME) in a new type of FeAl/PIN-PMN-PT heterostructure at room temperature, with a giant electrical modulation of magnetization for which the maximum relative magnetization change (ΔM/M) is up to 66%. The 109° ferroelastic domain switching in the PIN-PMN-PT and coupling with the ferromagnetic (FM) film via uniaxial anisotropy originating from the PIN-PMN-PT (011) surface are the key roles in converse ME effect. We also propose here a new, four-state memory through which it is possible to modify the remanent magnetism state by adjusting the electric field. This work represents a helpful approach to securing electric-writing magnetic-reading with low energy consumption for future high-density information storage applications.
Identification of MMP1 as a novel risk factor for intracranial aneurysms in ADPKD using iPSC models
Authors: Ameku Tomonaga , Taura Daisuke , Sone Masakatsu , Numata Tomohiro , Nakamura Masahiro , Shiota Fumihiko , Toyoda Taro , Matsui Satoshi , Araoka Toshikazu , Yasuno Tetsuhiko , Mae Shin-Ichi , Kobayashi Hatasu , Kondo Naoya , Kitaoka Fumiyo , Amano Naoki , Arai Sayaka , Ichisaka Tomoko , Matsuura Norio , Inoue Sumiko , Yamamoto Takuya , Takahashi Kazutoshi , Asaka Isao , Yamada Yasuhiro , Ubara Yoshifumi , Muso Eri , Fukatsu Atsushi , Watanabe Akira , Sato Yasunori , Nakahata Tatsutoshi , Mori Yasuo , Koizumi Akio , Nakao Kazuwa , Yamanaka Shinya , Osafune Kenji 
Published: July 2016

AbstractCardiovascular complications are the leading cause of death in autosomal dominant polycystic kidney disease (ADPKD), and intracranial aneurysm (ICA) causing subarachnoid hemorrhage is among the most serious complications. The diagnostic and therapeutic strategies for ICAs in ADPKD have not been fully established. We here generated induced pluripotent stem cells (iPSCs) from seven ADPKD patients, including four with ICAs. The vascular cells differentiated from ADPKD-iPSCs showed altered Ca2+ entry and gene expression profiles compared with those of iPSCs from non-ADPKD subjects. We found that the expression level of a metalloenzyme gene, matrix metalloproteinase (MMP) 1, was specifically elevated in iPSC-derived endothelia from ADPKD patients with ICAs. Furthermore, we confirmed the correlation between the serum MMP1 levels and the development of ICAs in 354 ADPKD patients, indicating that high serum MMP1 levels may be a novel risk factor. These results suggest that cellular disease models with ADPKD-specific iPSCs can be used to study the disease mechanisms and to identify novel disease-related molecules or risk factors.
Improved quality of life in patients with no-option critical limb ischemia undergoing gene therapy with DVC1-0101
Authors: Matsumoto Takuya , Tanaka Michiko , Yoshiya Keiji , Yoshiga Ryosuke , Matsubara Yutaka , Horiuchi-Yoshida Kumi , Yonemitsu Yoshikazu , Maehara Yoshihiko 
Published: July 2016

AbstractCritical limb ischemia (CLI) has a poor prognosis and adversely affects patients’ quality of life (QOL). Therapeutic angiogenesis may improve mobility, mortality and QOL in CLI patients. However, the effectiveness of gene therapy on such patients’ QOL is unknown. DVC1-0101, a non-transmissible recombinant Sendai virus vector expressing human fibroblast growth factor-2 gene, demonstrated safety and efficacy in a phase I/II study of CLI patients. We investigated the effects of DVC1-0101 on QOL in this cohort. QOL was assessed using the Short Form-36 health survey version 2 (SF-36) in 12 patients at pre-administration, 28 days and 3, 6 and 12 months post-treatment. We examined differences between pre and post-administration QOL scores and correlations between QOL scores and vascular parameters. Patients demonstrated low baselines scores on every SF-36 dimension. Post-treatment scores showed significant improvements in physical functioning at 3 and 6 months (P < 0.05), role-physical at 3, 6 and 12 months (P < 0.05), bodily pain at 1, 3, 6 and 12 months (P < 0.05), vitality at 1, 6 and 12 months (P < 0.05) and physical component summary at 6 and 12 months (P < 0.05). DVC1-0101-based gene therapy may improve QOL in CLI patients over a 6-month period.
Bystander autophagy mediated by radiation-induced exosomal miR-7-5p in non-targeted human bronchial epithelial cells
Authors: Song Man , Wang Yu , Shang Zeng-Fu , Liu Xiao-Dan , Xie Da-Fei , Wang Qi , Guan Hua , Zhou Ping-Kun 
Published: July 2016

AbstractRadiation-induced bystander effect (RIBE) describes a set of biological effects in non-targeted cells that receive bystander signals from the irradiated cells. RIBE brings potential hazards to adjacent normal tissues in radiotherapy, and imparts a higher risk than previously thought. Excessive release of some substances from irradiated cells into extracellular microenvironment has a deleterious effect. For example, cytokines and reactive oxygen species have been confirmed to be involved in RIBE process via extracellular medium or gap junctions. However, RIBE-mediating signals and intercellular communication pathways are incompletely characterized. Here, we first identified a set of differentially expressed miRNAs in the exosomes collected from 2 Gy irradiated human bronchial epithelial BEP2D cells, from which miR-7-5p was found to induce autophagy in recipient cells. This exosome-mediated autophagy was significantly attenuated by miR-7-5p inhibitor. Moreover, our data demonstrated that autophagy induced by exosomal miR-7-5p was associated with EGFR/Akt/mTOR signaling pathway. Together, our results support the involvement of secretive exosomes in propagation of RIBE signals to bystander cells. The exosomes-containing miR-7-5p is a crucial mediator of bystander autophagy.
Strategies for Teachers to Manage Stuttering in the Classroom: A Call for Research
Authors: Davidow Jason H. , Zaroogian Lisa , Garcia-Barrera Mauricio A. 
Published: July 2016

Purpose
This clinical focus article highlights the need for future research involving ways to assist children who stutter in the classroom.

Method
The 4 most commonly recommended strategies for teachers were found via searches of electronic databases and personal libraries of the authors. The peer-reviewed evidence for each recommendation was subsequently located and detailed.

Results
There are varying amounts of evidence for the 4 recommended teacher strategies outside of the classroom, but there are no data for 2 of the strategies, and minimal data for the others, in a classroom setting. That is, there is virtually no evidence regarding whether or not the actions put forth influence, for example, stuttering frequency, stuttering severity, participation, or the social, emotional, and cognitive components of stuttering in the classroom.

Conclusion
There is a need for researchers and speech-language pathologists in the schools to study the outcomes of teacher strategies in the classroom for children who stutter.
Impact of SiC semiconductors switching transition speed on insulation health state monitoring of traction machines
Authors: Zoeller Clemens , Vogelsberger Markus A. , Wolbank Thomas M. , Ertl Hans 
Published: July 2016

In modern traction propulsion applications, voltage source inverter (VSI) fed traction motors today operate very close to borderline conditions. With new emerging semiconductor technologies, higher inverter switching frequencies will be possible and high inverter dv/dt‐rates appear, resulting in transient overvoltages at the machine which increase the stress on the insulation system and lead to insulation degradation. Thus, insulation condition monitoring is getting more and more important to ensure a safe and reliable operation of traction motors in trains and locomotives, trams and so on. This study proposes an online insulation monitoring approach that is able to detect incipient insulation defects by evaluation of the motor transient current response on voltage pulses injected by standard inverter switching. Experimental results of this concept are obtained with tests on a 1.4 MW induction machine for railway application. Additionally, the influence of different dv/dt‐rates up to 20 kV/µs on the monitoring performance is verified using a VSI‐inverter equipped with SiC semiconductors.
Evaporation-triggered microdroplet nucleation and the four life phases of an evaporating Ouzo drop
Authors: Tan Huanshu , Diddens Christian , Lv Pengyu , Kuerten J. G. M. , Zhang Xuehua , Lohse Detlef 
Published: July 2016

Significance
The evaporation of an Ouzo droplet is a daily life phenomenon, but the outcome is amazingly rich and unexpected: Here we reveal the four different phases of its life with phase transitions in-between and the physics that govern this phenomenon. The Ouzo droplet may be seen as a model system for any ternary mixture of liquids with different volatilities and mutual solubilities. Our work may open up numerous applications in (medical) diagnostics and in technology, such as coating or for the controlled deposition of tiny amounts of liquids, printing of light-emitting diode (LED) or organic LED devices, or phase separation on a submicron scale.
A Tutorial in Consumer Research: Knowledge Creation and Knowledge Appreciation in Deductive-Conceptual Consumer Research
Authors: Janiszewski Chris , Labroo Aparna A. , Rucker Derek D. 
Published: July 2016

Abstract
A typical article in a top-tier business journal can require as much as $400,000 in academic labor costs (Terwiesch and Ulrich 2014). This estimate raises the question of what makes a contribution worthy of such a significant financial investment. How does an academic community determine the value of a contribution? We propose that two criteria inform judgments of value: the amount of knowledge creation and the amount of knowledge appreciation. Implicit in our view is the idea that researchers should know how to create valuable knowledge and be able to anticipate how much stakeholders will appreciate that knowledge. In this tutorial, we discuss knowledge creation, knowledge appreciation, and a framework that jointly represents these two sources of value. We hope that this framework will encourage scholars to engage in research activities that are valued by the scientific community.