bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jack Lindsay's Alienation
Authors: Harker Ben 
Published: August 2016

Abstract
Posterity has not been kind to the Australian-born polymath Jack Lindsay (1900–1990), a self-confessed ‘odd man out’ who published over one hundred and seventy books across a range of genres. This article asks what Lindsay wrote, why it has been forgotten and why we should care. It restores to view Lindsay's politico-cultural trajectory from his conversion to Marxism in 1936. It argues that Lindsay's searching and sceptical Marxism was a source of his prolific creativity and that he evolved a distinct and sometimes eccentric Marxist theoretical framework – a system with the concept of alienation at its core – within which his individual works are most legible. It argues that Lindsay's theoretical Marxism is not reducible to that of ‘British Communism’ or the ‘Old Left’, but exceeded and was mostly in tension with the Marxism of his party and its Soviet models, despite his political affiliation. It explains that Lindsay's oeuvre sank gradually from mainstream cultural visibility through the Cold War and that his conflicted but ongoing CP and Soviet-facing alignment alienated him from an emerging New Left with which he actually shared much theoretical ground. Estranged from his own party and largely dismissed by the New Left, his project disappeared through the cracks that opened during the traumatic realignments of the British Left in the post-1956 decade and remains largely absent from accounts of Marxist thought in Britain today. But though in many ways flawed, Lindsay's central project – tracing the processes of alienation through social formations, sifting human history for moments of resistance to that alienation, and attempting to prefigure a society in which values of creative production and communication are generalized across society as a whole – speaks loudly to ever-sharpening problems and deserves revisiting now.
Beyond Neo-Liberalism: The Trade Union Act 2016 and the Authoritarian State
Authors: Bogg Alan 
Published: August 2016

Abstract
The Trade Union Act 2016 marks a historically significant realignment in the ideological politics of trade union regulation. It represents a more authoritarian style of Conservative ideology and statecraft in the sphere of trade union regulation, and this is reflected in three main characteristics: (i) a repressive strategy of de-democratisation, undermining political resistance and stifling dissent in the democratic process; (ii) heavier reliance on direct State coercion, including the techniques of criminalisation, alongside the empowerment of employers to use civil law remedies against trade unions and workers in industrial action situations; and (iii) the elevation of social order in the regulation of strike activity. The article then considers the likely prospects of the legislation, and the wider ideological significance of this turn towards authoritarianism for Conservative political thought ‘beyond neo-liberalism’.
Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification
Authors: Qu Lianghuan , Wu Chunyan , Zhang Fei , Wu Yangyang , Fang Chuanying , Jin Cheng , Liu Xianqing , Luo Jie 
Published: August 2016

Abstract
Pectin synthesis and modification are vital for plant development, although the underlying mechanisms are still not well understood. Here, we report the functional characterization of the OsTSD2 gene, which encodes a putative methyltransferase in rice. All three independent T-DNA insertion lines of OsTSD2 displayed dwarf phenotypes and serial alterations in different zones of the root. These alterations included abnormal cellular adhesion and schizogenous aerenchyma formation in the meristematic zone, inhibited root elongation in the elongation zone, and higher lateral root density in the mature zone. Immunofluorescence (with LM19) and Ruthenium Red staining of the roots showed that unesterified homogalacturonan (HG) was increased in Ostsd2 mutants. Biochemical analysis of cell wall pectin polysaccharides revealed that both the monosaccharide composition and the uronic acid content were decreased in Ostsd2 mutants. Increased endogenous ABA content and opposite roles performed by ABA and IAA in regulating cellular adhesion in the Ostsd2 mutants suggested that OsTSD2 is required for root development in rice through a pathway involving pectin synthesis/modification. A hypothesis to explain the relationship among OsTSD2 , pectin methylesterification, and root development is proposed, based on pectin’s function in regional cell extension/division in a zone-dependent manner.
ABA is required for the accumulation of APX1 and MBF1c during a combination of water deficit and heat stress
Authors: Zandalinas Sara I. , Balfagón Damián , Arbona Vicent , Gómez-Cadenas Aurelio , Inupakutika Madhuri A. , Mittler Ron 
Published: August 2016

Abstract
Abscisic acid (ABA) plays a key role in plant acclimation to abiotic stress. Although recent studies suggested that ABA could also be important for plant acclimation to a combination of abiotic stresses, its role in this response is currently unknown. Here we studied the response of mutants impaired in ABA signalling ( abi1-1 ) and biosynthesis ( aba1-1 ) to a combination of water deficit and heat stress. Both mutants displayed reduced growth, biomass, and survival when subjected to stress combination. Focusing on abi1-1 , we found that although its stomata had an impaired response to water deficit, remaining significantly more open than wild type, its stomatal aperture was surprisingly reduced when subjected to the stress combination. Stomatal closure during stress combination in abi1-1 was accompanied by higher levels of H 2 O 2 in leaves, suggesting that H 2 O 2 might play a role in this response. In contrast to the almost wild-type stomatal closure phenotype of abi1-1 during stress combination, the accumulation of ascorbate peroxidase 1 and multiprotein bridging factor 1c proteins, required for acclimation to a combination of water deficit and heat stress, was significantly reduced in abi1-1 . Our findings reveal a key function for ABA in regulating the accumulation of essential proteins during a combination of water deficit and heat stress.
Dimensions and Role-Specific Mediators of Surrogate Trust in the ICU
Authors: Hutchison Paul J. , McLaughlin Katie , Corbridge Tom , Michelson Kelly N. , Emanuel Linda , Sporn Peter H. S. , Crowley-Matoka Megan 
Published: August 2016

Objective:
In the ICU, discussions between clinicians and surrogate decision makers are often accompanied by conflict about a patient’s prognosis or care plan. Trust plays a role in limiting conflict, but little is known about the determinants of trust in the ICU. We sought to identify the dimensions of trust and clinician behaviors conducive to trust formation in the ICU.

Design:
Prospective qualitative study.

Setting:
Medical ICU of a major urban university hospital.

Subjects:
Surrogate decision makers of intubated, mechanically ventilated patients in the medical ICU.

Measurements and Main Results:
Semistructured interviews focused on surrogates’ general experiences in the ICU and on their trust in the clinicians caring for the patient. Interviews were audio-recorded, transcribed verbatim, and coded by two reviewers. Constant comparison was used to identify themes pertaining to trust. Thirty surrogate interviews revealed five dimensions of trust in ICU clinicians: technical competence, communication, honesty, benevolence, and interpersonal skills. Most surrogates emphasized the role of nurses in trust formation, frequently citing their technical competence. Trust in physicians was most commonly related to honesty and the quality of their communication with surrogates.

Conclusions:
Interventions to improve trust in the ICU should be role-specific, since surrogate expectations are different for physicians and nurses with regard to behaviors relevant to trust. Further research is needed to confirm our findings and explore the impact of trust modification on clinician-family conflict.
Biomechanics of the Levator Aponeurosis
Authors: Repp Daniel J. , Rubinstein Tal J. , Herreid Peter A. , Sires Bryan S. 
Published: August 2016

Purpose:
To investigate biomechanical properties of the levator aponeurosis.

Methods:
Patients undergoing external levator resection for primary or revision correction of acquired ptosis were analyzed as primary and revision groups. Immediately postoperatively, the resected segments of the levator aponeurosis were stressed by adding sequential masses to the tissue. Specimen length was recorded at each stress level. Stress–strain diagrams were used to summarize how the tissue elongated in response to the external forces, because these diagrams correct for differences in specimen size. Representative specimens were analyzed histologically.

Results:
Twenty-two specimens from 14 patients in the primary group and 10 specimens from 7 patients in the revision group met inclusion criteria. In the primary group, the mean age was 66 years; 8 patients were women. In the revision group, the mean age was 69 years; 6 patients were women. Levator aponeurosis specimens in both the primary and revision group demonstrated proportional increases in length with increasing stress, demonstrating that the levator aponeurosis is extensile. Given the same amount of stress, the levator aponeurosis from primary specimens elongated more than revision specimens. Histologically, revision specimens exhibited increased collagen and haphazard, tangled elastin fibers.

Conclusions:
The levator aponeurosis obtained during surgical correction of acquired ptosis elongates in response to nominal external forces. This biomechanical property is important because the length of the aponeurosis may vary intraoperatively if variable forces are applied to the eyelid. This property might be related to connective tissue architecture and, specifically, fibrosis. Surgeons performing levator aponeurosis resection should be mindful to maintain a consistent amount of force on the levator aponeurosis when performing the resection to maximize intercase consistency.
A FLIC motility assay reveals myosin-6 coordination limited by actin filament buckling
Authors: Krenc Agata K. , Rokicka Jagoda J. , Rock Ronald S. 
Published: August 2016

ABSTRACTTeams of myosin motors carry out intracellular transport and contract the actin cytoskeleton. To fully understand the behavior of multi-myosin ensembles we need to know the properties of individual myosins and the mode of interaction between them. Current models of the interactions within the myosin complex treat the actin filament as a stiff rod, not contributing to the regulation of collective myosin dynamics. Here, we present data suggesting that force transduction through the actin filament is an important element of interaction within myosin-6 ensembles in vitro. Multiple myosin-6s coordinate their steps if they are separated by a short (and therefore high-force bearing) segment of actin. The measurements were performed using Fluorescence Interference Contrast Microscopy (FLIC) to measure small changes in the height of fluorescently labeled actin. Using FLIC, we assign the positions of myosins in a gliding filament assay geometry and measure their attachment time to actin. We also identify actin segments that are buckled or under tension. We show that myosin-6 holds actin about 10 nm above the surface. However, due to asynchronous myosin stepping, frequent buckles up to about 60 nm high appear. The buckle lifetime decreases as the distance between the myosin-6s is reduced, a sign of inter-motor coordination. Our data are consistent with coordinated stepping of closely spaced myosins, but uncoordinated motility with widely separated myosins where buckles can form. These features would be expected to operate on myosins in the cell, where motor spacing may vary considerably depending on the target organelle.SIGNIFICANCE STATEMENTMyosins are molecular motors that carry out intracellular transport. Interactions between the myosins are crucial for understanding their function. Using Fluorescence Interference Contrast (FLIC) microscopy we characterized the interaction between multiple myosin-6 motors immobilized to the surface of a slide and pulling the same actin filament. Our results point towards coordination of myosin steps as a mechanism governing the behavior of a multi-myosin complex. We also demonstrated the unique application of FLIC microscopy for highly parallel identification and measurement of single myosin motors in a gliding filament format. These features of FLIC enable a robust study of collective myosin dynamics.
Rapid expansion of immune-related gene families in the house fly,Musca domestica
Authors: Sackton Timothy B. , Lazzaro Brian P. , Clark Andrew G. 
Published: August 2016

AbstractThe house fly,Musca domestica, occupies an unusual diversity of potentially septic niches among sequenced Dipteran insects and is a vector of numerous diseases of humans and livestock. In the present study, we apply whole-transcriptome sequencing to identify genes whose expression is regulated in adult flies by bacterial infection. We then combine the transcriptomic data with analysis of rates of gene duplication and loss to provide insight into the evolutionary dynamics of immune-related genes. Genes up-regulated after bacterial infection are biased toward being evolutionarily recent innovations, suggesting the recruitment of novel immune components in theM. domesticaor ancestral Dipteran lineages. In addition, using new models of gene family evolution, we show that several different classes of immune-related genes, particularly those involved in either pathogen recognition or pathogen killing, are duplicating at a significantly accelerated rate on theM. domesticalineage relative to other Dipterans. Taken together, these results suggest that theM. domesticaimmune response includes an unusual diversity of genes, perhaps as a consequence of its lifestyle in septic environments.
Genomic insights into adaptive divergence and speciation among malaria vectors of theAnopheles niligroup
Authors: Fouet Caroline , Kamdem Colince , Gamez Stephanie , White Bradley J. 
Published: August 2016

AbstractOngoing speciation in most African malaria vectors gives rise to cryptic populations, which differ remarkably in their behaviour, ecology and capacity to vector malaria parasites. Understanding the population structure and the drivers of genetic differentiation among mosquitoes is critical for effective disease control because heterogeneity within species contribute to variability in malaria cases and allow fractions of vector populations to escape control efforts. To examine the population structure and the potential impacts of recent large-scale control interventions, we have investigated the genomic patterns of differentiation in mosquitoes belonging to theAnopheles niligroup, a large taxonomic group that diverged ∼3-Myr ago. Using 4343 single nucleotide polymorphisms (SNPs), we detected strong population structure characterized by highFSTvalues between multiple divergent populations adapted to different habitats within the Central African rainforest. Delineating the cryptic species within theAnopheles niligroup is challenging due to incongruence between morphology, ribosomal DNA and SNP markers consistent with incomplete lineage sorting and/or interspecific gene flow. A very high proportion of loci are fixed (FST= 1) within the genome of putative species, which suggests that ecological and/or reproductive barriers are maintained by strong selection on a substantial number of genes.
Extensive genetic diversity among populations of the malaria mosquito Anopheles moucheti revealed by population genomics
Authors: Fouet Caroline , Kamdem Colince , Gamez Stephanie , White Bradley J. 
Published: August 2016

AbstractMalaria vectors are exposed to intense selective pressures due to large-scale intervention programs that are underway in most African countries. One of the current priorities is therefore to clearly assess the adaptive potential of Anopheline populations, which is critical to understand and anticipate the response mosquitoes can elicit against such adaptive challenges. The development of genomic resources that will empower robust examinations of evolutionary changes in all vectors including currently understudied species is an inevitable step toward this goal. Here we constructed double-digest Restriction Associated DNA (ddRAD) libraries and generated 6461 Single Nucleotide Polymorphisms (SNPs) that we used to explore the population structure and demographic history of wild-caught Anopheles moucheti from Cameroon. The genome-wide distribution of allelic frequencies among samples best fitted that of an old population at equilibrium, characterized by a weak genetic structure and extensive genetic diversity, presumably due to a large long term effective population size. Estimates of FST and Linkage Disequilibrium (LD) across SNPs reveal a very low genetic differentiation throughout the genome and the absence of segregating LD blocks among populations, suggesting an overall lack of local adaptation. Our study provides the first investigation of the genetic structure and diversity in An. moucheti at the genomic scale. We conclude that, despite a weak genetic structure, this species has the potential to challenge current vector control measures and other rapid anthropogenic and environmental changes thanks to its great genetic diversity.
Cytoarchitectonic similarity is a wiring principle of the human connectome
Authors: Goulas Alexandros , Werner René , Beul Sarah F , Säring Dennis , Heuvel Martijn van den , Triarhou Lazaros C , Hilgetag Claus C 
Published: August 2016

AbstractUnderstanding the wiring diagram of the human cerebral cortex is a fundamental challenge in neuroscience. Elemental aspects of its organization remain elusive. Here we examine which structural traits of cortical regions, particularly their cytoarchitecture and thickness, relate to the existence and strength of inter-regional connections. We use the architecture data from the classic work of von Economo and Koskinas and state-of-the-art diffusion-based connectivity data from the Human Connectome Project. Our results reveal a prominent role of the cytoarchitectonic similarity of supragranular layers for predicting the existence and strength of connections. In contrast, cortical thickness similarity was not related to the existence or strength of connections. These results are in line with findings for non-human mammalian cerebral cortices, suggesting overarching wiring principles of the mammalian cerebral cortex. The results invite hypotheses about evolutionary conserved neurobiological mechanisms that give rise to the relation of cytoarchitecture and connectivity in the human cerebral cortex.
A complete tool set for molecular QTL discovery and analysis
Authors: Delaneau Olivier , Ongen Halit , Brown Andrew , Fort Alexandre , Panousis Nikolaos , Dermitzakis Emmanouil 
Published: August 2016

AbstractPopulation scale studies combining genetic information with molecular phenotypes (e.g. gene expression) become a standard to dissect the effects of genetic variants onto organismal. This kind of datasets requires powerful, fast and versatile methods able to discover molecular Quantitative Trait Loci (molQTL). Here we propose such a solution, QTLtools, a modular framework that contains multiple methods to prepare the data, to discover proximal and distal molQTLs and to finally integrate them with GWAS variants and functional annotations of the genome. We demonstrate its utility by performing a complete expression QTL study in a few and easy-to-perform steps. QTLtools is open source and available athttps://gtltools.github.io/gtltools/
Corporate social responsibility reporting in the mining sector of Tanzania
Authors: Lauwo Sarah George , Otusanya Olatunde Julius , Bakre Owolabi 
Published: August 2016

Purpose– The purpose of this paper is to contribute to the ongoing debate on governance, accountability, transparency and corporate social responsibility (CSR) in the mining sector of a developing country context. It examines the reporting practices of the two largest transnational gold-mining companies in Tanzania in order to draw attention to the role played by local government regulations and advocacy and campaigning by nationally organised non-governmental organisations (NGOs) with respect to promoting corporate social reporting practices.Design/methodology/approach– The paper takes a political economy perspective to consider the serious implications of the neo-liberal ideologies of the global capitalist economy, as manifested in Tanzania’s regulatory framework and in NGO activism, for the corporate disclosure, accountability and responsibility of transnational companies (TNCs). A qualitative field case study methodology is adopted to locate the largely unfamiliar issues of CSR in the Tanzanian mining sector within a more familiar literature on social accounting. Data for the case study were obtained from interviews and from analysis of documents such as annual reports, social responsibility reports, newspapers, NGO reports and other publicly available documents.Findings– Analysis of interviews, press clips and NGO reports draws attention to social and environmental problems in the Tanzanian mining sector, which are arguably linked to the manifestation of the broader crisis of neo-liberal agendas. While these issues have serious impacts on local populations in the mining areas, they often remain invisible in mining companies’ social disclosures. Increasing evidence of social and environmental ills raises serious questions about the effectiveness of the regulatory frameworks, as well as the roles played by NGOs and other pressure groups in Tanzania.Practical implications– By empowering local NGOs through educational, capacity building, technological and other support, NGOs’ advocacy, campaigning and networking with other civil society groups can play a pivotal role in encouraging corporations, especially TNCs, to adopt more socially and environmentally responsible business practices and to adhere to international and local standards, which in turn may help to improve the lives of many poor people living in developing countries in general, and Tanzania in particular.Originality/value– This paper contributes insights from gold-mining activities in Tanzania to the existing literature on CSR in the mining sector. It also contributes to political economy theory by locating CSR reporting within the socio-political and regulatory context in which mining operations take place in Tanzania. It is argued that, for CSR reporting to be effective, robust regulations and enforcement and stronger political pressure must be put in place.