bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of a natural DNMT1 inhibitor, peperomin E, in rat plasma by UFLC‐MS/MS and method application in a pharmacokinetic study
Authors: Wang Xin‐zhi , Wen Hong‐mei , Chai Chuan , Zhang Wen‐ying , Gao Ming , Liu Rui , Wu Hao , Liang Jing‐yu 
Published: January 2017

AbstractPeperomin E (PepE), a naturally occurring secolignan isolated from Peperomia dindygulensis, has drawn much attention recently owing to its anticancer and DNA methyltransferase 1 (DNMT1) inhibitory activity. Here, a simple and sensitive ultra‐fast liquid chromatography–tandem mass spectrometry method was developed and validated for the determination of PepE in rat plasma for the first time. Samples were prepared by simple protein precipitation. Separation was performed on an XBridge™ C18 column using a mobile phase of acetonitrile and 0.1% (v/v) aqueous formic acid. PepE and the internal standard arctigenin were detected in a positive‐ion mode using multiple reaction monitoring of the transitions at m/z 413.2 → 261.0 and 373.2 → 137.2, respectively. The calibration curve for PepE was linear over the range of concentrations of 1.46–6000 ng/mL, with a lower limit of quantitation of 1.46 ng/mL. Both intra‐ and interday precisions were within 11.05%, and the accuracy ranged from −11.5 to 5.51%. The extraction recovery and matrix effect were within acceptable limits. Stability tests showed that PepE remained stable throughout the analytical procedure. The validated method was then used to analyze the pharmacokinetics of PepE administered to rats orally (12.5 and 25 mg/kg) or intravenously (6.25 and 12.5 mg/kg).
Overexpression of TNKS1BP1 in lung cancers and its involvement in homologous recombination pathway of DNA double‐strand breaks
Authors: Tan Wei , Guan Hua , Zou Lian‐Hong , Wang Yu , Liu Xiao‐Dan , Rang Wei‐Qing , Zhou Ping‐Kun , Pei Hua‐Dong , Zhong Cai‐Gao 
Published: January 2017

AbstractTNKS1BP1 is a member of the poly(ADP‐ribose) polymerase (PARP) superfamily. Our previous studies have demonstrated that TNKS1BP1 plays an important role in DNA damage response. But whether and how TNKS1BP1 associates with cancer is still not clear. Here, we found that TNKS1BP1 was upregulated in human lung adenocarcinoma (LAC) tissues, and was associated with poor overall survival (OS) in LAC patients. Dysregulation of TNKS1BP1 affected the sensitivity of A549 cells to several DNA damage agents including cisplatin, bleomycin, and ionizing radiation. Mechanically, overexpression of TNKS1BP1 increased the accumulation of S phase cells, which was accompanied by a decrease in M phase cells. More importantly, we found TNKS1BP1 regulated genome stability, mainly through affecting the homologous recombination pathway of DNA double‐strand breaks by inhibiting the RAD51 foci formation. Overall, our study indicates that, in LAC, aberrant expressions of TNKS1BP1 are common events, and overexpression of TNKS1BP1 might affect outcomes of cancer patients to chemotherapy and radiotherapy.
A New Triazine‐Based Covalent Organic Framework for High‐Performance Capacitive Energy Storage
Authors: Bhanja Piyali , Bhunia Kousik , Das Sabuj K. , Pradhan Debabrata , Kimura Ryuto , Hijikata Yuh , Irle Stephan , Bhaumik Asim 
Published: January 2017

AbstractThe new covalent organic framework material TDFP‐1 was prepared through a solvothermal Schiff base condensation reaction of the monomers 1,3,5‐tris‐(4‐aminophenyl)triazine and 2,6‐diformyl‐4‐methylphenol. Owing to its high specific surface area of 651 m2 g−1, extended π conjugation, and inherent microporosity, TDFP‐1 exhibited an excellent energy‐storage capacity with a maximum specific capacitance of 354 F g−1 at a scan rate of 2 mV s−1 and good cyclic stability with 95 % retention of its initial specific capacitance after 1000 cycles at 10 A g−1. The π‐conjugated polymeric framework as well as ionic conductivity owing to the possibility of ion conduction inside the micropores of approximately 1.5 nm make polymeric TDFP‐1 a favorable candidate as a supercapacitor electrode material. The electrochemical properties of this electrode material were measured through cyclic voltammetry, galvanic charge–discharge, and electrochemical impedance spectroscopy, and the results indicate its potential for application in energy‐storage devices.
Metal–Phenolic Carbon Nanocomposites for Robust and Flexible Energy‐Storage Devices
Authors: Oh Jun Young , Jung Yeonsu , Cho Young Shik , Choi Jaeyoo , Youk Ji Ho , Fechler Nina , Yang Seung Jae , Park Chong Rae 
Published: January 2017

AbstractFuture electronics applications such as wearable electronics depend on the successful construction of energy‐storage devices with superior flexibility and high electrochemical performance. However, these prerequisites are challenging to combine: External forces often cause performance degradation, whereas the trade‐off between the required nanostructures for strength and electrochemical performance only results in diminished energy storage. Herein, a flexible supercapacitor based on tannic acid (TA) and carbon nanotubes (CNTs) with a unique nanostructure is presented. TA was self‐assembled on the surface of the CNTs by metal–phenolic coordination bonds, which provides the hybrid film with both high strength and high pseudocapacitance. Besides 17‐fold increased mechanical strength of the final composite, the hybrid film simultaneously exhibits excellent flexibility and volumetric capacitance.
Comparison of three methods for analyzing loureirin B and human serum albumin interaction using capillary electrophoresis
Authors: Zhang Yuelin , Sha Yijie , Qian Kai , Chen Xu , Chen Qin 
Published: January 2017

Loureirin B (LB), a bioactive drug, is widely used in the treatment of biological diseases. However, due to its poor solution in water, it is important to find the approach which helps LB to specific biological targets. As the most abundant protein in plasma, HSA plays the role of a carrier of numerous drug ligand. Thus, the interaction between LB and HSA was explored by ACE, CE frontal analysis, and pressure‐mediated ACE under simulated physiological conditions (pH 7.4). The binding constants were calculated as 13.14 × 104 L/mol, 7.00 × 104 L/mol, and 2.78 × 104 L/mol for each method, respectively. At the same time, the binding site number (n = 1.429) could be only calculated by the CE frontal analysis method. Furthermore, good experimental repeatability was obtained by pressure‐mediated ACE with RSDs for retention times and peak areas within 2.149 and 1.228, respectively.
Impact of ASD Traits on Treatment Outcomes of Eating Disorders in Girls
Authors: Stewart Catherine S. , McEwen Fiona S. , Konstantellou Anna , Eisler Ivan , Simic Mima 
Published: January 2017

AbstractEvidence links high levels of Autism Spectrum Disorder Traits in women with chronicity of anorexia nervosa. This study reports through clinical audit the impact of ASD traits on treatment outcomes of girls who were referred for treatment in a specialist eating disorder service. Presence of current, but not early childhood, ASD traits was elevated in comparison with previously reported community samples. Current ASD traits were correlated with emotional disorders and with need for treatment augmentation (psychiatric inpatient or day patient admission), but this relationship was not significant after the contribution of depression had been controlled for. There was no difference in Morgan Russell Outcomes at discharge for those with high and low current ASD traits. Parent‐reported ASD‐related developmental difficulties were associated with attenuated change in self‐reported cognitive symptoms of AN. This study highlights the need for further understanding of the aetiology, diagnostic significance and predictive utility for future relapse of elevated ASD traits in childhood eating disorders. Copyright © 2017 John Wiley & Sons, Ltd and Eating Disorders Association.
Particle‐size evidence of barrier estuary regime as a new proxy for ENSO climate variability
Authors: Clarke David W. , Boyle John F. , Plater Andrew J. 
Published: January 2017

AbstractWe investigate a new proxy for ENSO climate variability based on particle‐size data from long‐term, coastal sediment records preserved in a barrier estuary setting. Corresponding ~4–8 year periodicities identified from Wavelet analysis of particle‐size data from Pescadero Marsh in Central Coast California and rainfall data from San Francisco reflect established ENSO periodicity, as further evidenced in the Multivariate ENSO Index (MEI), and thus confirms an important ENSO control on both precipitation and barrier regime variability. Despite the fact that barrier estuary mean particle size is influenced by coastal erosion, precipitation and streamflow, balanced against barrier morphology and volume, it is encouraging that considerable correspondence can also be observed in the time series of MEI, regional rainfall and site‐based mean particle size over the period 1871–2008. This correspondence is, however, weakened after c.1970 by temporal variation in sedimentation rate and event‐based deposition. These confounding effects are more likely when: (i) accommodation space may be a limiting factor; and (ii) particularly strong El Niños, e.g. 1982/1983 and 1997/1998, deposit discrete >cm‐thick units during winter storms. The efficacy of the sediment record of climate variability appears not to be compromised by location within the back‐barrier setting, but it is limited to those El Niños that lead to barrier breakdown. For wider application of this particle size index of ENSO variability, it is important to establish a well‐resolved chronology and to sample the record at the appropriate interval to characterize deposition at a sub‐annual scale. Further, the sample site must be selected to limit the influence of decreasing accommodation space through time (infilling) and event‐based deposition. It is concluded that particle‐size data from back‐barrier sediment records have proven potential for preserving evidence of sub‐decadal climate variability, allowing researchers to explore temporal and spatial patterns in phenomena such as ENSO. Copyright © 2017 John Wiley & Sons, Ltd.
A systematic review of maternal near miss and mortality due to postpartum hemorrhage
Authors: Maswime Salome , Buchmann Eckhart 
Published: January 2017

AbstractBackgroundPostpartum hemorrhage (PPH) is the principal direct cause of maternal mortality worldwide. Analysis of maternal near miss could increase understanding of survival among women with life‐threatening PPH.ObjectivesTo determine the near‐miss ratio and maternal mortality index for PPH globally.Search strategyA prevalence systematic review was conducted of English‐language articles published from 1995 to 2014. Suitable articles were identified from the Scopus, PubMed, Embase, and Grey Literature databases. The main search terms used were “maternal near‐miss” and “severe acute maternal morbidity.”Selection criteriaNear‐miss studies and audits describing the severe maternal outcome rate for PPH were included.Data collection and analysisData were extracted from eligible publications. Quantitative analysis and narrative synthesis were used.Main resultsFor 26 included studies, the median near‐miss ratio for PPH was 3 per 1000 live births. The mortality index for PPH was 6.6% (range 0.0%–40.7%). The mortality index was highest in low‐income countries and lower middle‐income countries. Overall, PPH was the most frequent contributor to obstetric hemorrhage, with atonic uterus identified as the main cause.ConclusionsWomen in low‐income countries and lower middle‐income countries have an increased likelihood of severe PPH and of dying from PPH‐related consequences.
Levels of heavy metals and their binding protein metallothionein in type 2 diabetics with kidney disease
Authors: Raudenska Martina , Dvorakova Veronika , Pacal Lukas , Chalasova Katarina , Kratochvilova Monika , Gumulec Jaromir , Ruttkay‐Nedecky Branislav , Zitka Ondrej , Kankova Katerina , Adam Vojtech , Masarik Michal 
Published: January 2017

AbstractHyperglycemia, a major metabolic disturbance present in diabetes, promotes oxidative stress. Activation of antioxidant defense is an important mechanism to prevent cell damage. Levels of heavy metals and their binding proteins can contribute to oxidative stress. Antiradical capacity and levels of metallothionein (MT), metals (zinc and copper), and selected antioxidants (bilirubin, cysteine, and glutathione) were determined in 70 type 2 diabetes mellitus (T2DM) subjects and 80 healthy subjects of Caucasian origin. Single nucleotide polymorphism (rs28366003) in MT gene was detected. Antiradical capacity, conjugated bilirubin, and copper were significantly increased in diabetics, whereas MT and glutathione were decreased. Genotype AA of rs28366003 was associated with higher zinc levels in the diabetic group. The studied parameters were not influenced by renal function. This is the first study comprehensively investigating differences in MT and metals relevant to oxidative stress in T2DM. Ascertained differences indicate increased oxidative stress in T2DM accompanied by abnormalities in non‐enzymatic antioxidant defense systems.
Orientation of the Mitotic Spindle in the Development of Tubular Organs
Authors: Zhong Tao , Zhou Jun 
Published: January 2017

ABSTRACTFormation of organs that consist primarily or exclusively of tubes is essential for metazoan development. Increasing evidence suggests that the morphogenesis and homeostasis of these tubular organs depend on proper orientation of the mitotic spindle during cell division. Consequently, improper spindle orientation can perturb spatial arrangement of daughter cells, resulting in congenital malformations or dysfunctions of tubular organs. Over the past decade, the association of spindle misorientation with brain diseases and cancer has been extensively studied. However, few studies have explored the effects of spindle misorientation on developmental disorders impacting tubular organs. Here, we examine and interpret recent literature that shows how tubular organ development relies on proper spindle orientation and discuss how defects in this cellular process are associated with the pathogenesis of tubular organ diseases. J. Cell. Biochem. 118: 1630–1633, 2017. © 2017 Wiley Periodicals, Inc.
Small RNAome profiling from human skeletal muscle: novel miRNAs and their targets associated with cancer cachexia
Authors: Narasimhan Ashok , Ghosh Sunita , Stretch Cynthia , Greiner Russell , Bathe Oliver F. , Baracos Vickie , Damaraju Sambasivarao 
Published: January 2017

AbstractBackgroundMicroRNAs (miRs) are small non‐coding RNAs that regulate gene (mRNA) expression. Although the pathological role of miRs have been studied in muscle wasting conditions such as myotonic and muscular dystrophy, their roles in cancer cachexia (CC) are still emerging.ObjectivesThe objectives are (i) to profile human skeletal muscle expressed miRs; (ii) to identify differentially expressed (DE) miRs between cachectic and non‐cachectic cancer patients; (iii) to identify mRNA targets for the DE miRs to gain mechanistic insights; and (iv) to investigate if miRs show potential prognostic and predictive value.MethodsStudy subjects were classified based on the international consensus diagnostic criteria for CC. Forty‐two cancer patients were included, of which 22 were cachectic cases and 20 were non‐cachectic cancer controls. Total RNA isolated from muscle biopsies were subjected to next‐generation sequencing.ResultsA total of 777 miRs were profiled, and 82 miRs with read counts of ≥5 in 80% of samples were retained for analysis. We identified eight DE miRs (up‐regulated, fold change of ≥1.4 at P < 0.05). A total of 191 potential mRNA targets were identified for the DE miRs using previously described human skeletal muscle mRNA expression data (n = 90), and a majority of them were also confirmed in an independent mRNA transcriptome dataset. Ingenuity pathway analysis identified pathways related to myogenesis and inflammation. qRT‐PCR analysis of representative miRs showed similar direction of effect (P < 0.05), as observed in next‐generation sequencing. The identified miRs also showed prognostic and predictive value.ConclusionsIn all, we identified eight novel miRs associated with CC.
Effect of fermentation temperature on hydrogen production from xylose and the succession of hydrogen‐producing microflora
Authors: Qiu Chunsheng , Yuan Ping , Sun Liping , Wang Shaopo , Lo Shanglien , Zhang Dandan 
Published: January 2017

AbstractBACKGROUNDHydrogen production through anaerobic dark fermentation is considered to be a potential biological process for xylose utilization. Temperature is one of the most important environmental factors, however, most studies have been carried out over a small temperature range. Batch tests were carried out to investigate the temperature effect on hydrogen production from xylose using a mixed culture over a wide temperature range (35–65°C). Hydrogen production, metabolite distribution and dynamics of microbial communities were investigated.RESULTSHydrogen‐producing cultures were successfully enriched at each tested temperature. Two peaks of fermentation temperature for hydrogen production were observed at 35 and 55°C (1.11 and 1.30 mol‐H2 mol−1‐xyloseconsumed, respectively). Butyrate and acetate were the major liquid metabolites at 35–60°C. While at 65°C the main by‐product was ethanol. Polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) analysis indicated that Clostridium species were dominant at 35–40°C, while Thermoanaerobacterium dominated at 45–60°C. Both species were found at 65°C, but with lowest microbial community diversity.CONCLUSIONHydrogen production efficiency was mainly affected by the liquid metabolite distributions, which depended mainly upon the temperature. Several microbial community structures were formed at mesophilic, transition, thermophilic and extreme‐thermophilic conditions, resulting in different metabolic pathways of xylose and hydrogen production capacity. © 2017 Society of Chemical Industry
An assessment of historical and projected future hydro‐climatic variability and extremes over southern watersheds in the Canadian Prairies
Authors: Bonsal Barrie R. , Cuell Charles , Wheaton Elaine , Sauchyn David J. , Barrow Elaine 
Published: January 2017

ABSTRACTSince human activities and ecosystem health require adequate, reliable water supplies, hydro‐climatic variability and extremes pose serious threats to society and the environment. Previous studies have shown that the Canadian Prairies normally experience considerable hydro‐climatic variability, including periodic droughts and excessive moisture conditions, which are mainly caused by mid‐tropospheric circulations that disrupt expected precipitation and temperature patterns. However, no investigations have specifically focused on both past and future hydro‐climatology over watersheds within the region. Evaluation of the Standardized Precipitation Evapotranspiration Index reveals considerable inter‐annual and decadal‐scale variability over the study regions of the Oldman and Swift Current Creek Watersheds, with no discernible trends during the last ∼100 years. There is also an indication of increased variability since the mid‐1980s. Assessment of the 500 hPa circulation patterns associated with identified hydro‐climatic extremes shows that major droughts are related with higher frequencies of distinctive ridging patterns over the Prairie region, and lower incidences of zonal/troughing patterns (the former significantly increasing over the last 60 years). Excessive moisture conditions have opposite patterns. Projections from two (a drier/warmer and wetter/cooler) Regional Climate Models indicate an uncertain future in the selected watersheds ranging from a substantial increase in drought with a higher degree of inter‐annual variability, to relatively no change from current conditions. Furthermore, future changes to key atmospheric circulations suggest that those patterns associated with extreme dry conditions will continue in the future and in some cases, increase in frequency. Results from this analysis have improved the understanding of historical hydro‐climatic extremes in western Canada and have provided insight into potential future occurrences of these extremes as driven by changes to key surface climate and synoptic‐scale atmospheric circulation patterns.
Neuromuscular compensation mechanisms in vocal fold paralysis and paresis
Authors: Dewan Karuna , Vahabzadeh‐Hagh Andrew , Soofer Donna , Chhetri Dinesh K. 
Published: January 2017

Objectives/HypothesisVocal fold paresis and paralysis are common conditions. Treatment options include augmentation laryngoplasty and voice therapy. The optimal management for this condition is unclear. The objective of this study was to assess possible neuromuscular compensation mechanisms that could potentially be used in the treatment of vocal fold paresis and paralysis.Study DesignIn vivo canine model.MethodsIn an in vivo canine model, we examined three conditions: 1) unilateral right recurrent laryngeal nerve (RLN) paresis and paralysis, 2) unilateral superior laryngeal nerve (SLN) paralysis, and 3) unilateral vagal nerve paresis and paralysis. Phonatory acoustics and aerodynamics were measured in each of these conditions. Effective compensation was defined as improved acoustic and aerodynamic profile.ResultsThe most effective compensation for all conditions was increasing RLN activation and decreasing glottal gap. Increasing RLN activation increased the percentage of possible phonatory conditions that achieved phonation onset. SLN activation generally led to decreased number of total phonation onset conditions within each category. Differential effects of SLN (cricothyroid [CT] muscle) activation were seen. Ipsilateral SLN activation could compensate for RLN paralysis; normal CT compensated well in unilateral SLN paralysis; and in vagal paresis/paralysis, contralateral SLN and RLN displayed antagonistic relationships.ConclusionsMethods to improve glottal closure should be the primary treatment for large glottal gaps. Neuromuscular compensation is possible for paresis. This study provides insights into possible compensatory mechanisms in vocal fold paresis and paralysis.Level of EvidenceNA Laryngoscope, 127:1633–1638, 2017
Change in loneliness after intervention with cochlear implants or hearing aids
Authors: Contrera Kevin J. , Sung Yoon K. , Betz Joshua , Li Lingsheng , Lin Frank R. 
Published: January 2017

ObjectiveTo investigate the impact of hearing aid (HA) and cochlear implant (CI) use on loneliness in adults.Study DesignProspective observational cohort study.MethodsOne hundred and thirteen adults, aged ≥ 50 years, with postlingual hearing loss and receiving routine clinical care at a tertiary academic medical center, were evaluated with the University of California at Los Angeles Loneliness Scale before and 6 and 12 months after intervention with HAs or CIs. Change in score was assessed using linear mixed effect models adjusted for age; gender; education; and history of hypertension, diabetes, and smoking.ResultsSignificant improvements in loneliness scores were observed in CI users from baseline to 6 months (−3.79 [95% confidence interval): −5.73, −1.85], P <.001) and baseline to 12 months (−3.26 [95% confidence interval: −5.66, −0.87], P =.007). We did not observe a significant improvement in loneliness scores in HA users from baseline to 6 months (−0.83 [95% confidence interval: −2.68, 1.02], P =.381) or baseline to 12 months (−0.34 [95% confidence interval: −2.77, −2.10], P = .007). The most substantial increases were observed in individuals with the lowest baseline scores.ConclusionTreatment of hearing loss with CIs results in a significant reduction in loneliness symptoms. This improvement was not observed with HAs. We observed differential effects of treatment depending on the baseline loneliness score, with the greatest improvements observed in individuals with the most loneliness symptoms at baseline.Level of Evidence2b. Laryngoscope, 127:1885–1889, 2017