bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceptions of adults with type 1 diabetes toward diabetes-specific quality of life measures: a survey-based qualitative exploration
Authors: Holmes-Truscott Elizabeth , Schipp Jasmine , Cooke Debbie D. , Hendrieckx Christel , Coates Elizabeth J. , Heller Simon R. , Speight Jane 
Published: September 2024

Abstract
Background
Diabetes-specific quality of life (QoL) questionnaires are commonly used to assess the impact of diabetes and its management on an individual’s quality of life. While several valid and reliable measures of diabetes-specific QoL exist, there is no consensus on which to use and in what setting. Furthermore, there is limited evidence of their acceptability to people with diabetes. Our aim was to explore perceptions of adults with type 1 diabetes (T1D) toward five diabetes-specific QoL measures.

Methods
Adults (aged 18 + years) with T1D living in Australia or the United Kingdom (UK) were eligible to take part in ‘YourSAY: QoL’, an online cross-sectional survey. Recruitment involved study promotion on diabetes-related websites and social media, as well as direct invitation of people with T1D via a hospital client list (UK only). In random order, participants completed five diabetes-specific QoL measures: Audit of Diabetes-Dependent Quality of Life (ADDQoL-19); Diabetes Care Profile: Social and Personal Factors subscale (DCP); DAWN Impact of Diabetes Profile (DIDP); Diabetes-Specific Quality of Life Scale: Burden Subscale (DSQoLS); Diabetes Quality of Life Questionnaire (Diabetes QOL-Q). They were invited to provide feedback on each questionnaire in the form of a brief free-text response. Responses were analysed using inductive, thematic template analysis.

Results
Of the N = 1,946 adults with T1D who completed the survey, 20% (UK: n = 216, Australia: n = 168) provided qualitative responses about ≥ 1 measure. All measures received both positive and negative feedback, across four themes: (1) clarity and ease of completion, e.g., difficulty isolating impact of diabetes, dislike of hypothetical questions, and preference for ‘not applicable’ response options; (2) relevance and comprehensiveness, e.g., inclusion of a wide range of aspects of life to improve personal relevance; (3) length and repetition, e.g., length to be balanced against respondent burden; (4) framing and tone, e.g., preference for respectful language and avoidance of extremes.

Conclusions
These findings suggest opportunities to improve the relevance and acceptability of existing diabetes-specific QoL measures, and offer considerations for developing new measures, which need to be better informed by the preferences of people living with diabetes.
Genetic markers of late radiation toxicity in the era of image-guided radiotherapy: lower toxicity rates reduce the predictive value of γ-H2AX foci decay ratio in patients undergoing pelvic radiotherapy
Authors: Nuijens Anna C. , Oei Arlene L. , Koster Lisa , Hoebe Ron A. , Franken Nicolaas A.P. , Rasch Coen R.N. , Stalpers Lukas J.A. 
Published: September 2024

Abstract
Background
A predictive assay for late radiation toxicity would allow more personalized treatment planning, reducing the burden of toxicity for the more sensitive minority, and improving the therapeutic index for the majority. In a previous study in prostate cancer patients, the γ-H2AX foci decay ratio (γ-FDR) was the strongest predictor of late radiation toxicity. The current study aimed to validate this finding in a more varied group of patients with pelvic cancer. Additionally, the potential correlation between the γ-FDR and patient-reported outcomes was investigated.

Methods
Prostate and gynecological cancer patients with ≥ 24 months of follow-up were included in the current analysis. Toxicity was evaluated by physician (CTCAE version 4) and patient (EORTC questionnaires). γ-FDRs were determined in ex vivo irradiated lymphocytes. Correlation between γ-FDR and toxicity was assessed using both linear and logistic regression analyses. The highest toxicity grade recorded during follow-up was used. The association between global quality of life and γ-FDR was tested by comparing the change in quality of life over time in patients with γ-FDR < or ≥ 3.41, a previously established threshold.

Results
Eighty-eight patients were included. Physician-assessed and patient-reported cumulative grade ≥ 2 toxicity was 25% and 29%, respectively; which is much lower than in the previous cohort (i.e., 51% CTCAE grade ≥ 2). Patients with toxicity exhibited less favorable dose-volume parameters. In men, these parameters showed significant improvement compared to the previous cohort. The proportion of patients with a low γ-FDR increased with severity of toxicity, but this trend was not statistically significant. In addition, a γ-FDR < 3.41 was not correlated with the development of moderate to severe toxicity. Post-treatment decline in global quality of life was minimal, and similar for patients with γ-FDR < or ≥ 3.41.

Conclusions
In the present study, the γ-H2AX foci decay ratio could not be validated as a predictor of late radiation toxicity in patients with pelvic cancer. Improved radiotherapy techniques with smaller irradiated bladder and bowel volumes have probably resulted in less toxicities. Future studies on genetic markers of toxicity should be powered on these lower incidences. We further recommend taking persistency, next to severity, into consideration.
Environmental bacterial load during surgical and ultrasound procedures in a Swedish small animal hospital
Authors: Alsing-Johansson Todd , Bergström Karin , Sternberg-Lewerin Susanna , Bergh Anna , Östlund Emma , Penell Johanna 
Published: September 2024

Abstract
Background
Environmental bacteria in animal healthcare facilities may constitute a risk for healthcare-associated infections (HAI). Knowledge of the bacterial microflora composition and factors influencing the environmental bacterial load can support tailored interventions to lower the risk for HAI. The aims of this study were to: (1) quantify and identify environmental bacteria in one operating room (OR) and one ultrasound room (UR) in a small animal hospital, (2) compare the bacterial load to threshold values suggested for use in human healthcare facilities, (3) characterise the genetic relationship between selected bacterial species to assess clonal dissemination, and (4) investigate factors associated with bacterial load during surgery.
Settle plates were used for passive air sampling and dip slides for surface sampling. Bacteria were identified by Matrix Assisted Laser Desorption—Time Of Flight. Antimicrobial susceptibility was determined by broth microdilution. Single nucleotide polymorphism-analysis was performed to identify genetically related isolates. Linear regression was performed to analyse associations between observed explanatory factors and bacterial load.

Results
The bacterial load on settle plates and dip slides were low both in the OR and the UR, most of the samples were below threshold values suggested for use in human healthcare facilities. All settle plates sampled during surgery were below the threshold values suggested for use in human clean surgical procedures.
Staphylococcus spp. and Micrococcus spp. were the dominating species. There was no indication of clonal relationship among the sequenced isolates. Bacteria carrying genes conveying resistance to disinfectants were revealed.
Air change and compliance with hygiene routines were sufficient in the OR. No other factors possibly associated with the bacterial load were identified.

Conclusions
This study presents a generally low bacterial load in the studied OR and UR, indicating a low risk of transmission of infectious agents from the clinical environment. The results show that it is possible to achieve bacterial loads below threshold values suggested for use in human healthcare facilities in ORs in small animal hospitals and thus posing a reduced risk of HAI. Bacteria carrying genes conveying resistance to disinfectants indicates that resistant bacteria can persist in the clinical environment, with increased risk for HAI.
Absence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes
Authors: Choi Jiyoung , Wu Yumei , Park Daehun 
Published: September 2024

AbstractATG9A is the only integral membrane protein among core autophagy-related (ATG) proteins. We previously found that ATG9A does not co-assemble into synaptophysin-positive vesicles, but rather, localizes to a distinct pool of vesicles within synapsin condensates in both fibroblasts and nerve terminals. The endocytic origin of these vesicles further suggests the existence of different intracellular sorting or segregation mechanisms for ATG9A and synaptophysin in cells. However, the precise underlying mechanism remains largely unknown. In this follow-up study, we investigated the endosomal localization of these two proteins by exploiting the advantages of a Rab5 mutant that induces the formation of enlarged endosomes. Notably, ATG9A and synaptophysin intermix perfectly and do not segregate on giant endosomes, indicating that the separation of these two proteins is not solely caused by the inherent properties of the proteins, but possibly by other unknown factors.
Enhancing the Flexural Capacity of Deteriorated Low-Strength Prestressed Concrete Beam Using Near-Surface Mounted Post-Tensioned Carbon Fiber-Reinforced Polymer Bar
Authors: Cho Sanghyeon , Chung Wonseok , Jung Woo-tai , Park Jong-sup , Lee Heeyoung 
Published: September 2024

AbstractThis study aimed to address the critical issue of age deterioration in prestressed concrete (PSC) structures by investigating the strengthening of aged PSC structures using a near-surface mounted (NSM) post-tensioned carbon fiber-reinforced polymer (CFRP). A total of nine PSC beams, each with a length of 6.5 m, were fabricated for a four-point bending test. Various experimental parameters were taken into account, including the strengthening method, compressive strength of concrete in the PSC beam, and the prestressing force of the PSC beam. The results indicated that the NSM post-tensioned CFRP strengthening system proved more efficient when compared to the NSM non-post-tensioned CFRP strengthening system. The flexural capacity of the NSM post-tensioned CFRP strengthening system, under the deteriorated low-strength PSC beam, increased by up to 30.9% compared to the PSC reference beam. Additionally, the experimental results were compared to a finite-element analysis, and a parametric study was conducted to examine the material properties of the PSC beam. Consequently, the NSM post-tensioned CFRP strengthening system is expected to be an effective solution for addressing the issue of deteriorated low-strength PSC structures.
Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp (Ctenopharyngodon idella): possibly via activating the ROS-AMPK-autophagy axis
Authors: He Yuanlin , Wu Pei , Jiang Weidan , Liu Yang , Jin Xiaowan , Ren Hongmei , Zhang Ruinan , Zhou Xiaoqiu , Feng Lin 
Published: September 2024

Abstract
Background
Methionine (Met) is the only sulfur-containing amino acid among animal essential amino acids, and methionine deficiency (MD) causes tissue damage and cell death in animals. The common modes of cell death include apoptosis, autophagy, pyroptosis, necroptosis. However, the studies about the major modes of cell death caused by MD have not been reported, which worth further study.

Methods
Primary hepatocytes from grass carp were isolated and treated with different doses of Met (0, 0.5, 1, 1.5, 2, 2.5 mmol/L) to examine the expression of apoptosis, pyroptosis, autophagy and necroptosis-related proteins. Based on this, we subsequently modeled pyroptosis using lipopolysaccharides and nigericin sodium salt, then autophagy inhibitors chloroquine (CQ), AMP-activated protein kinase (AMPK) inhibitors compound C (CC) and reactive oxygen species (ROS) scavengers N-acetyl-L-cysteine (NAC) were further used to examine the expression of proteins related to pyroptosis, autophagy and AMPK pathway in MD-treated cells respectively.

Results
MD up-regulated B-cell lymphoma protein 2 (Bax), microtubule-associated protein 1 light chain 3 II (LC3 II), and down-regulated the protein expression levels of B-cell lymphoma-2 (Bcl-2), sequestosome 1 (p62), cleaved-caspase-1, cleaved-interleukin (IL)-1β, and receptor-interacting protein kinase (RIP) 1 in hepatocytes, while it did not significantly affect RIP3. In addition, MD significantly increased the protein expression of liver kinase B1 (LKB1), p-AMPK, and Unc-51-like kinase 1 (ULK1) without significant effect on p-target of rapamycin. Subsequently, the use of CQ increased the protein expression of NOD-like receptor thermal protein domain associated protein 3 (NLRP3), cleaved-caspase-1, and cleaved-IL-1β inhibited by MD; the use of CC significantly decreased the protein expression of MD-induced LC3 II and increased the protein expression of MD-suppressed p62; then the use of NAC decreased the MD-induced p-AMPK protein expression.

Conclusion
MD promoted autophagy and apoptosis, but inhibited pyroptosis and necroptosis. MD inhibited pyroptosis may be related regarding the promotion of autophagy. MD activated AMPK by inducing ROS production which in turn promoted autophagy. These results could provide partial theoretical basis for the possible mechanisms of Met in ensuring the normal structure and function of animal organs. Furthermore, ferroptosis is closely related to redox states, it is worth investigating whether MD affects ferroptosis in hepatocytes.
Ubiquitin modification in the regulation of tumor immunotherapy resistance mechanisms and potential therapeutic targets
Authors: Hong Zihang , Liu Furong , Zhang Zhanguo 
Published: September 2024

AbstractAlthough immune checkpoint-based cancer immunotherapy has shown significant efficacy in various cancers, resistance still limits its therapeutic effects. Ubiquitination modification is a mechanism that adds different types of ubiquitin chains to proteins, mediating protein degradation or altering their function, thereby affecting cellular signal transduction. Increasing evidence suggests that ubiquitination modification plays a crucial role in regulating the mechanisms of resistance to cancer immunotherapy. Drugs targeting ubiquitination modification pathways have been shown to inhibit tumor progression or enhance the efficacy of cancer immunotherapy. This review elaborates on the mechanisms by which tumor cells, immune cells, and the tumor microenvironment mediate resistance to cancer immunotherapy and the details of how ubiquitination modification regulates these mechanisms, providing a foundation for enhancing the efficacy of cancer immunotherapy by intervening in ubiquitination modification.
Predicting of hepatic steatosis in living liver donor via CT liver attenuation index (LAI) and fibroscan controlled attenuation parameter (CAP) correlation with biopsy result
Authors: Mahfouz Somaia Mohammed Ali , Mohamed Fatma Salah eldin , Chalabi Nivine Abdel Moneium , Abdel-Rahman Hazem Ibrahim 
Published: September 2024

Abstract
Background
The most prevalent persistent parenchymatous liver alterations in healthy individuals are thought to be hepatic steatosis. The liver biopsy is the most crucial procedure for the identification and measurement of hepatic steatosis. By identifying the liver attenuation index (LAI) at CT image with fibroscan controlled attenuation parameter (CAP), hepatic steatosis can be evaluated without the risk of liver resection.

Objective
Using liver biopsy histological analysis as a reference standard, to examine the precision of the CT liver attenuation index (LAI) and fibroscan controlled attenuation parameter (CAP) for quantitative evaluation of macrovesicular steatosis in living related liver donors.

Methods
In this cross-sectional study, comparing the CT liver attenuation index & fibroscan controlled attenuation parameter with liver biopsy result for the detection of the steatosis in subject's candidate for liver living donors, 50 subjects were conducted at Ain Shams Specialized Hospital and other private hospitals over about 2 years.

Results
Our study reported that liver attenuation index of 9 is the cutoff value in post-contrast CT images with sensitivity 100% and specificity 80% that make it a very good method to exclude donor to have steatosis ≥ 15%, which mean that if donor had LAI index < 9, we can safely do proceed do liver biopsy. Our study reported that CAP measurement had an AUROC OF 0.780, for detecting steatosis ≥ 15%, with sensitivity is only 60% with specificity as CT LAI of 80%, our results consider low compared to other studies, that could be due to small number of donors in our study with steatosis ≥ 15% (five cases from 50 donors) unlike the other studies.

Conclusion
When used to estimate the amount of liver fat in liver donors, the examined CAP and CT indices worked equally. But according to multivariate analysis, the only factor strongly linked with hepatic steatosis in a living donors was the CT LAI index. We contend that the combination of CT LS attenuation index and CAP allows for the detection of the degree of hepatic steatosis and can be used as an option to liver biopsy, reserving liver biopsy for those with positive steatosis donors.
Genomic insights into cpb2-positive Clostridium perfringens and the potential biological function of cpb2 gene
Authors: Wu Ke , Yuan Yuan , Fang Mingjin , Liu Yiwen , Yang Danjiao , Zhang Likun , Tian Xin , Dou Leina , Ye Dongyang , Fox Edward M. , Wang Juan , Yang Zengqi 
Published: September 2024

AbstractClostridium perfringens, capable of causing intestinal infections in both animals and humans, represents a significant public health concern. This study aimed to assess the occurrence of the beta2 toxin-coding gene cpb2 in C. perfringens from various host species and to explore the genetic contexts of this gene. The results showed an enrichment of cpb2 in pig-derived C. perfringens. A comparative analysis of the detection rates of cpb2 and pCP13-like plasmids revealed that the cpb2 gene itself, rather than the pCP13-like plasmids, caused the enrichment. Sequence comparison of cpb2-positive pCP13-like plasmids showed that cpb2 was located on the cpb2-hp-transcriptional regulator (PadR family) segment. Despite the diverse plasmid structures of pCP13-like plasmids, the cpb2-hp-transcriptional regulator (PadR family) segment was consistently observed in all cpb2-positive C. perfringens strains, suggesting the potential transmission of the cpb2 gene on this specific genetic segment. Additionally, phylogenetic analysis of the C. perfringens strains harboring pCP13-like plasmids, as well as 31 pCP13-like plasmids, indicated that cpb2 did not affect the evolutionary relationship of either pCP13-like plasmids or C. perfringens. Genetic markers, particularly those located on mobile genetic elements (MGEs), that can help bacteria survive in external environments are more readily enriched in the population. The high prevalence of cpb2 in pig-derived strains indicated that it might confer a selective advantage, enhancing the survival and persistence of C. perfringens in the pig intestine. In conclusion, our study elucidated the genetic context, host tropism and potential biological functions of cpb2, which can provide references for further research.
All psychiatric disorders are equal, but some are more equal than others! An unconscious bias that calls for precision terminology
Authors: Arjmand Shokouh , Guldager Matti Bock , Wegener Gregers 
Published: September 2024

SummaryWe address the unconsciously biased perception of psychiatric disorders, highlighting a hierarchical perspective that favours certain diagnoses over others. We aim to uncover reasons for these inequities, emphasising the need for a shift toward pathophysiology-based nomenclature that can promote equal support for each disorder, enhance treatment adherence and encourage open discussions.
Aharonov-Bohm effect mediated by massive photons
Authors: Kang Kicheon 
Published: September 2024

Abstract
Virtual photons play an essential role in the locally realistic description of the Aharonov-Bohm interference. We show that the effect of virtual photons in the interferometer is manifested by a change in their spectrum. In particular, when a vacuum is confined between two ideal conducting plates, the photons obey the two-dimensional Proca equation, the wave equation with finite effective mass. This results in a short-range interaction between a test charge and a magnetic flux, and hence the Aharonov-Bohm effect is reduced exponentially at a large distance between the two bodies. On the other hand, a semiclassical description is also possible, and this raises the interesting question of how to prove the physical reality of virtual photons.
Machine Learning for K-Adaptability in Two-Stage Robust Optimization
Authors: Julien Esther , Postek Krzysztof , Birbil Ş. İlker 
Published: September 2024

Two-stage robust optimization problems constitute one of the hardest optimization problem classes. One of the solution approaches to this class of problems is K-adaptability. This approach simultaneously seeks the best partitioning of the uncertainty set of scenarios into K subsets and optimizes decisions corresponding to each of these subsets. In a general case, it is solved using the K-adaptability branch-and-bound algorithm, which requires exploration of exponentially growing solution trees. To accelerate finding high-quality solutions in such trees, we propose a machine learning-based node selection strategy. In particular, we construct a feature engineering scheme based on general two-stage robust optimization insights, which allows us to train our machine learning tool on a database of resolved branch-and-bound trees and to apply it as is to problems of different sizes and/or types. We experimentally show that using our learned node selection strategy outperforms a vanilla, random node selection strategy when tested on problems of the same type as the training problems as well as in cases when the K-value or the problem size differs from the training ones. History: Accepted by Andrea Lodi, Area Editor for Design & Analysis of Algorithms—Discrete. Funding: This work was supported by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek [Grants OCENW.GROOT.2019.015 and VI.Veni.191E.035]. Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2022.0314 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2022.0314 ). The complete IJOC Software and Data Repository is available at https://informsjoc.github.io/ .
An existence result for
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Authors: Alreshidi Bandar , Hai Dang Dinh 
Published: September 2024

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