bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aurora kinase targeting in lung cancer reduces KRAS-induced transformation
Authors: dos Santos Edmilson Ozorio , Carneiro-Lobo Tatiana Correa , Aoki Mateus Nobrega , Levantini Elena , Bassères Daniela Sanchez 
Published: February 2016

Abstract
Background
Activating mutations in KRAS are prevalent in lung cancer and have been causally linked to the oncogenic process. However, therapies targeted to oncogenic RAS have been ineffective to date and identification of KRAS targets that impinge on the oncogenic phenotype is warranted. Based on published studies showing that mitotic kinases Aurora A (AURKA) and B (AURKB) cooperate with oncogenic RAS to promote malignant transformation and that AURKA phosphorylates RAS effector pathway components, the aim of this study was to investigate whether AURKA and AURKB are KRAS targets in lung cancer and whether targeting these kinases might be therapeutically beneficial.

Methods
In order to determine whether oncogenic KRAS induces Aurora kinase expression, we used qPCR and western blotting in three different lung cell-based models of gain- or loss-of-function of KRAS. In order to determine the functional role of these kinases in KRAS-induced transformation, we generated KRAS-positive A549 and H358 cells with stable and inducible shRNA-mediated knockdown of AURKA or AURKB and evaluated transformation in vitro and tumor growth in vivo. In order to validate AURKA and/or AURKB as therapeutically relevant KRAS targets in lung cancer, we treated A549 and H358 cells, as well as two different lung cell based models of gain-of-function of KRAS with a dual Aurora kinase inhibitor and performed functional in vitro assays.

Results
We determined that KRAS positively regulates AURKA and AURKB expression. Furthermore, in KRAS-positive H358 and A549 cell lines, inducible knockdown of AURKA or AURKB, as well as treatment with a dual AURKA/AURKB inhibitor, decreased growth, viability, proliferation, transformation, and induced apoptosis in vitro. In addition, inducible shRNA-mediated knockdown of AURKA in A549 cells decreased tumor growth in vivo. More importantly, dual pharmacological inhibiton of AURKA and AURKB reduced growth, viability, transformation, and induced apoptosis in vitro in an oncogenic KRAS-dependent manner, indicating that Aurora kinase inhibition therapy can specifically target KRAS-transformed cells.

Conclusions
Our results support our hypothesis that Aurora kinases are important KRAS targets in lung cancer and suggest Aurora kinase inhibition as a novel approach for KRAS-induced lung cancer therapy.
MiR-302a/b/c/d cooperatively sensitizes breast cancer cells to adriamycin via suppressing P-glycoprotein(P-gp) by targeting MAP/ERK kinase kinase 1 (MEKK1)
Authors: Zhao Lin , Wang Yan , Jiang Longyang , He Miao , Bai Xuefeng , Yu Lifeng , Wei Minjie 
Published: February 2016

Abstract
Background
The importance of individual microRNAs (miRNAs) in tumor has been established in different cancers. However, their association with tumor chemoresistance has not been fully understood. Previously, we found two novel MDR-associated microRNAs (miRNAs). In this report, we investigated the combined effects of miRNA gene cluster in chemoresistance of breast cancer.

Methods
This study was performed in two different breast cancer cell lines (MCF-7 and MCF-7/ADR). The levels of miRNAs and mRNA expression were determined by using Quantitative Real-Time PCR. Western blotting was used to detect the levels of protein molecules. Cell viability was assessed by MTS assay. Bioinformatics and Luciferase reporter assay was performed to examine miRNA binding to the 3′-UTR of target genes.

Results
The miR-302S family including miR-302a, miR-302b, miR-302c, and miR-302d was significantly down-regulated in P-glycoprotein (P-gp)-overexpressing MCF-7/ADR cells. Overexpression of miR-302 increased intracellular accumulation of ADR and sensitized breast cancer cells to ADR. Most importantly, miR-302S produced stronger effects than each individual member alone. The four miRNAs cooperatively downregulate P-gp expression in regulating drug sensitivity. However, our results showed that the suppression of P-gp expression by miR-302 is not through typical miRNA-mediated mRNA degradation but at the level of protein and transcription. Further studies identified MAP/ERK kinase kinase 1 (MEKK1) as a direct and functional target of miR-302. miR-302 showed combinatorial effects on MKEE1 repression and MEKK1-mediated ERK pathway. The suppression of P-gp by miR-302 was reversed by MEKK1 overexpression.

Conclusion
Our results indicate that miR-302 cooperatively sensitizes breast cancer cells to adriamycin via suppressing P-glycoprotein by targeting MEKK1 of ERK pathway. miR-302 gene cluster may be a potential target for reversing P-gp-mediated chemoresistance in breast cancer.
Distribusi mikroplastik pada sedimen di Muara Badak, Kabupaten Kutai Kartanegara
Authors: Sari Dewi Intan , Aditya Budiarsa Anugrah , Ramadhan Ritonga Irwan 
Published: February 2016

Abstract. The distribution of microplastic was studied in the Muara Badak districts Kutai Kartanegara regency in May 2015. Sediment samples were taken from four stations that represent residential, in mangrove, near the beach and away from residential areas. Sediment samples were taken using the pipe at a depth of 0-10 cm - 10-20 cm, then the observed and calculated abundance of microplastic. The results showed that the type microplastic found is a fragment, film and fiber. At Station 1 found fragments about 100.2-201.3 particle/kg, the film about 69.6-79.9 particles/kg and fiber about 43.1-50.9 particles/kg, the stations 2 found of fragments about 146.5-238.8 particle/kg, the film about 53.2-81.9 particles/kg and fiber about 48.8-75.5 particles/kg, stations 3 found of fragments about 204.2-207.9 particles/kg, the film about 107.7-126.5 particle/kg and fiber about 26.1-39.9 particles/kg, station 4 found of fragments about 167.6-220 particle/kg, the film about 59.7-69.5 particle/ kg and fiber about 47.5-55.3 particles/kg. Keywords: Depth; Microplastic; Muara Badak; Sediment Abstrak. Distribusi mikroplastik diteliti di Kecamatan Muara Badak Kabupaten Kutai Kartanegara pada bulan Mei 2015. Sampel sedimen diambil dari 4 stasiun yang mewakili pemukiman penduduk, dekat sungai, dekat pantai dan jauh dari pemukiman penduduk. Sampel sedimen diambil menggunakan pipa paralon pada kedalaman 0-10 cm – 10-20 cm, kemudian diamati dan dihitung kelimpahan mikroplastik. Hasil penelitian menunjukan bahwa jenis mikroplastik yang ditemukan adalah fragmen, film dan fiber. Pada stasiun 1 ditemukan fragmen berkisar 100,2-201,3 partikel/kg, film berkisar 69,6-79,9 partikel/kg dan fiber berkisar 43,1-50,9 partikel/kg, stasiun 2 ditemukan fragmen berkisar 146,5-238,8 partikel/kg, film berkisar 53,2-81,9 partikel/kg dan fiber berkisar 48,8-75,2 partikel/kg, stasiun 3 ditemukan fragmen berkisar 204,2-207,9 partikel/kg, film berkisar 107,7-126,5 partikel/kg dan fiber berkisar 26,1-39,9 partikel/kg, stasiun 4 ditemukan fragmen berkisar 167,6-220 partikel/kg, film berkisar 59,7-69,5partikel/kg dan fiber berkisar 47,5-55,3 partikel/kg.Kata kunci: Kedalaman; Mikroplastik; Muara Badak; Sedimen
The Mosaic Mitral Valve Bioprosthesis: A Long-Term Clinical and Hemodynamic Follow-Up
Authors: Celiento Michele , Blasi Stefania , De Martino Andrea , Pratali Stefano , Milano Aldo D. , Bortolotti Uberto 
Published: February 2016

We reviewed the cases of 100 patients (mean age, 73 ± 10 yr; 64 men) who had mitral valve replacement with a Medtronic Mosaic porcine bioprosthesis from 1995 through 2011. The mean New York Heart Association (NYHA) class was 3 ± 0.7, and 52 patients were in atrial fibrillation. Prosthetic sizes were chiefly 27 mm (50 patients) and 29 mm (40 patients). Follow-up ended in December 2012 and is 97% complete, with a cumulative duration of 611 patient-years (mean duration, 6 ± 4.6 yr; maximum, 17.7 yr).
The early mortality rate was 10% (6% in elective patients); late deaths occurred in 31 patients (5 valve-related). Actuarial survival rates at 5, 10, and 15 years were 74% ± 5%, 50% ± 6%, and 37% ± 8%. The mean NYHA class in survivors was 1.4 ± 0.6 (P <0.0001). Thromboembolic episodes occurred in 4 patients, with an actuarial freedom at 15 years of 91% ± 5%. No cases of endocarditis were observed. Four patients needed reoperation, 2 for structural failure, and 1 each for perivalvular leakage and valve thrombosis. Actuarial freedom from structural failure and from reoperation, respectively, was 93% ± 5% and 91% ± 5% at 15 years. Echocardiographic follow-up in 24 patients with 27-mm prostheses showed a mean gradient of 5 ± 1.7 mmHg and an effective orifice area of 1.57 ± 0.3 cm2; in 16 patients with 29-mm prostheses, the mean gradient was 4.5 ± 1.9 mmHg, and the effective orifice area, 1.63 ± 0.4 cm2.
During nearly 17 years of follow-up, the Mosaic bioprosthesis has shown good overall clinical and hemodynamic performance after mitral valve replacement.
Angiosarcoma Causing Cardiac Constriction Late after Radiation Therapy for Breast Carcinoma
Authors: Sharma Ashwini , DeValeria Patrick A. , Scherber Robyn M. , Sugrue Gavin , McCullough Ann E. , Panse Prasad M. , Mookadam Farouk 
Published: February 2016

Therapeutic radiotherapy rarely causes sarcoma, and this occurs years after completion of the intended treatment. In treating breast carcinoma, careful planning in the application of modern radiotherapeutic techniques usually can shield the heart and pericardium.
We report a rare case of angiosarcoma of the pericardium, which presented in a 41-year-old woman as constrictive pericarditis 8 years after irradiation for cancer of the left breast. To our knowledge, this is only the 2nd report of angiosarcoma of the pericardium after radiotherapy.
Primary Pulmonary Valve Leiomyosarcoma in a 35-Year-Old Woman
Authors: Vakilian Farveh , Shabestari Mahmoud M. , Poorzand Hoorak , Teshnizi Mohammad Abbasi , Allahyari Abolghasem , Memar Bahram 
Published: February 2016

Primary cardiac leiomyosarcomas are rare, with a high incidence of local recurrence. Herein, we report the case of a 35-year-old woman who was admitted with right ventricular failure and suspected pulmonary embolism. Upon echocardiography, we detected a mass in the pulmonary trunk that involved the pulmonary valve and led to valvular stenosis.
The optimal protocol for treating these tumors is as yet unclear. Complete resection can rarely be achieved. However, palliative surgery is usually undertaken because many patients present with mechanical obstruction, such as significant pulmonary stenosis.
Epicardial Adipose Tissue Thickness Is an Independent Predictor of Critical and Complex Coronary Artery Disease by Gensini and Syntax Scores
Authors: Erkan Aycan Fahri , Tanindi Asli , Kocaman Sinan Altan , Ugurlu Murat , Tore Hasan Fehmi 
Published: February 2016

Epicardial adipose tissue thickness is associated with the severity and extent of atherosclerotic coronary artery disease. We prospectively investigated whether epicardial adipose tissue thickness is related to coronary artery disease extent and complexity as denoted by Gensini and Syntax scores, and whether the thickness predicts critical disease.
After performing coronary angiography in 183 patients who had angina or acute myocardial infarction, we divided them into 3 groups: normal coronary arteries, noncritical disease (≥1 coronary lesion with <70% stenosis), and critical disease (≥1 coronary lesion with <70% stenosis). We used transthoracic echocardiography to measure epicardial adipose tissue thickness, then calculated Gensini and Syntax scores by reviewing the angiograms.
Mean thicknesses were 4.3 ± 0.9, 5.2 ± 1.5, and 7.5 ± 1.9 mm in patients with normal coronary arteries, noncritical disease, and critical disease, respectively (P <0.001). At progressive thicknesses (<5, 5–7, and >7 mm), mean Gensini scores were 4.1 ± 5.5, 19.8 ± 15.6, and 64.9 ± 32.4, and mean Syntax scores were 4.7 ± 5.9, 16.6 ± 8.5, and 31.7 ± 8.7, respectively (both P <0.001). Thickness had strong and positive correlations with both scores (Gensini, r =0.82, P <0.001; and Syntax, r =0.825, P <0.001). The cutoff thickness value to predict critical disease was 5.75 mm (area under the curve, 0.875; 95% confidence interval, 0.825–0.926; P <0.001).
Epicardial adipose tissue thickness is independently related to coronary artery disease extent and complexity as denoted by Gensini and Syntax scores, and it predicts critical coronary artery disease.
Cone Reconstruction of Atypical Ebstein Anomaly Associated with Right Ventricular Apical Hypoplasia
Authors: Reddin Gemma , Poterucha Joseph T. , Dearani Joseph A. , Warnes Carole A. , Cetta Frank 
Published: February 2016

Cone reconstruction for tricuspid valve repair has revolutionized the surgical treatment of Ebstein anomaly. We present the case of a 58-year-old woman with atypical Ebstein anomaly and right ventricular apical hypoplasia who was spared from palliative shunt physiology by our use of cone reconstruction.
Compared with other techniques, cone reconstruction of the tricuspid valve more closely replicates normal valvular anatomy and function. This surgical procedure can be applied to many anatomic variations of Ebstein anomaly, as in our patient's apparently unique instance of atypical Ebstein anomaly with right ventricular apical hypoplasia.
Classification of the Bid/No Bid Criteria – Factor Analysis
Authors: Leśniak A. 
Published: February 2016

Abstract For the construction company, tendering is the most popular way of acquiring contracts. The decision to participate in the tender needs to be made carefully, as it affects the condition of the company and is an important aspect in its quest for success. The bid/no bid decision making is a complex process involving a number of factors. The research carried out so far has mainly concerned the identification of the various kinds of influences on contractors’ bidding decisions. The researchers, on the basis of contractors’ opinions, created rank lists in an attempt to categorize the factors. In this paper the author employs factor analysis which belongs to basic methods of multi-dimensional data analysis. The paper’s aim is first to depict an output set of observed variables, that is bid/no bid factors, in terms of a smaller set of latent variables which cannot be directly observed and then to interpret the dependencies between them.
Disparities in Regional Economic Development in Romania
Authors: Oțil Maria Daniela , Miculescu Andra , Cismaș Laura Mariana 
Published: February 2016

Abstract
The issue of economic disparities within the European Union economies is not new, it is actually a topical issue. Unfortunately, the EU enlargement has determined an even stronger deepening of the regional disparities, because in the absence of adequate regional development policies, the financial instruments have proved to be ineffective. Recent studies show that the economic crisis has increased regional disparities in the European Union countries, influencing the most important regions, especially the economically less advanced ones, the significant regional differences being identified at the NUTS 3 level. Based on these issues, the present paper tries to answer the following questions: 1. How extended are the regional disparities in Romania and how did they evolve over the period 1998-2012? 2. How did the economic crisis influence disparities? Which territorial units were more affected?
In order to measure regional inequalities the Hoover index was used as well as the coefficient of variation, and the indicator for assessing the level of development as well as for highlighting regional disparities was GDP per capita. The analysis and interpretation of the results provide an overview of the situation at the regional level in Romania
Workplace Harassment: A Context Analysis for the Romanian Managers
Authors: Nestian Andrei Stefan , Tita Silviu Mihail , Arustei Carmen Claudia 
Published: February 2016

Abstract
The present article describes the key elements of the workplace harassment issues in general and from the Romanian official documents and regulations perspective. The divergences between these regulations and the international approaches on workplace harassment are analysed, considering the practical implications for managers and human resources departments, in order to reveal the needs for adaptation of the Romanian official framework related to this issue. Findings show that treating the harassment as discrimination, and not as form of psychological violence, creates the biggest challenges for managers
Biological methods used to assess surface water quality
Authors: Szczerbiñska Natalia , Gałczyñska Małgorzata 
Published: February 2016

Abstract
In accordance with the guidelines of the Water Framework Directive 2000/60 (WFD), both ecological and chemical statuses determine the assessment of surface waters. The profile of ecological status is based on the analysis of various biological components, and physicochemical and hydromorphological indicators complement this assessment. The aim of this article is to present the biological methods used in the assessment of water status with a special focus on bioassay, as well as to provide a review of methods of monitoring water status. Biological test methods include both biomonitoring and bioanalytics. Water biomonitoring is used to assess and forecast the status of water. These studies aim to collect data on water pollution and forecast its impact. Biomonitoring uses organisms which are characterized by particular vulnerability to contaminants. Bioindicator organisms are algae, fungi, bacteria, larval invertebrates, cyanobacteria, macroinvertebrates, and fish. Bioanalytics is based on the receptors of contaminants that can be biologically active substances. In bioanalytics, biosensors such as viruses, bacteria, antibodies, enzymes, and biotests are used to assess degrees of pollution.
Hematological and blood gas profiles of triploid Siberian sturgeon (Acipenser baerii Brandt)
Authors: Rożyñski Maciej , Demska-Zakęś Krystyna , Fopp-Bayat Dorota 
Published: February 2016

Abstract
The aim of the study was to determine the impact of triploidization on hematological and blood gas parameters in Siberian sturgeon, Acipenser baerii Brandt. The study material was comprised of juvenile Siberian sturgeon specimens with total body lengths of 22.3 ± 2.9 cm and mean body weights of 75.3 ± 40.7 g. Triploidization was confirmed to result in significant changes in the hematological profile of Siberian sturgeon. In comparison to the diploid group, the fish with higher levels of ploidy exhibited lower erythrocyte counts, increased hemoglobin concentration, and increased values of mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. A significant decrease in the percentage share of lymphocytes and an increase in the percentage share of granulocytes in the peripheral blood were also observed, which indicate disturbances in the immune response (particularly in the adaptive immune system) and greater susceptibility to stress. Triploidization did not, however, cause significant differences in the blood gas profile.
On the Price of Commitment Assets in a General Equilibrium Model with Credit Constraints and Tempted Consumers
Authors: Woźny Łukasz 
Published: February 2016

AbstractWe analyze a three period production economy, where households exhibit problems of self-control and face credit constraints. Apart from liquid assets, a single commitment (illiquid) asset is available that allows to commit to a planned consumption path. We compare general equilibrium allocations of the two models: one, where households choices are determined using Gul and Pesendorfer (2001, “Temptation and Self-Control.”
The Compensation and Benefits System: Private Companies vs. Budgetary System
Authors: Aldea Bogdan 
Published: February 2016

Abstract
The economic evolution of the last decades has entailed major changes concerning the Human Resource Management practices, in both private and budgetary sector of Romania. In order to maintain efficiency and market competitivity, a company must adapt to these changes by altering its human resource strategy since managers are encountering increasingly complex challenges as to attracting, motivating and retaining employees. Such an objective might be accomplished by elaborating a competitive system of employee compensation. This article focuses on the stage reached by the Human Resource Management in the compensation and benefits system of the private and budgetary sector. For the latter, we are focusing on the romanian system of higher education. This study demonstrates that the compensation and benefits system which is implemented in romanian universities is limited and can offer few opportunities as compared to the one implemented by private companies. It is worth saying that the legal system has a negative impact in this matter.
Recyclable graphene-supported palladium nanocomposites for Suzuki coupling reaction
Authors: Upadhyay Praveenkumar Ramprakash , Srivastava Vivek 
Published: February 2016

AbstractIn the search of new materials for developing clean catalytic organic reactions, our team engineered graphene based palladium (Pd) nanocomposites and tested them as catalysts for ionic liquid mediated Suzuki cross coupling reaction. The effects of various reaction parameters, such as solvent, base, time and temperature, were investigated for the Suzuki reaction. The optimized protocol was applied to a wide range of substituted aryl/hetroaryl halides along with various aryl boronic acids and afforded the corresponding reaction products in good to excellent yield. The proposed Pd/reduced graphene oxide (rGO) catalyzed Suzuki reaction protocol was also exploited for the synthesis of unique indazole derivatives. The developed catalytic system circumvents the use of phosphine ligands, with an added advantage of easy Pd catalyst recovery up to eight times.
Self-Love and Neighbor-Love in Kierkegaard’s Ethics
Authors: Aumann Antony 
Published: February 2016

AbstractKierkegaard faces a dilemma. On the one hand, he endorses the biblical command to love our neighbors as ourselves. As such, he thinks self-love and neighbor-love should be symmetrical, similar in kind as well as degree. On the other hand, he recommends relating to others and ourselves differently. We are to be lenient, charitable and forgiving when dealing with others; the opposite when dealing with ourselves. To resolve this tension, I argue that being more stringent with ourselves is not a moral ideal for Kierkegaard. It is a gambit designed to rehabilitate us from our tendency toward the opposite extreme.
“Love” Among the Post-Socratics
Authors: Carlsson Ulrika 
Published: February 2016

AbstractVictor Eremita proposes that the reader understand parts I and II of Either/Or as parties in a dialogue; most readers in fact view II as a devastating reply to I. I suggest that part I be read as a reaction or follow-up to Kierkegaard’s dissertation. Much of part I presents reflective characters who are aware of their freedom but reluctant or unable to adopt the ethical life. The modern Antigone and the Silhouettes are sisters of Alcibiades-failed students of Socrates. I articulate and defend their modes of loving, which are significantly different from Don Giovanni’s and Johannes the Seducer’s purely aesthetic approaches to love. Such feminine love, I argue, dwells in the disputed territory between passion and action, substance and freedom, the aesthetic and the ethical. Antigone’s love is a passion she both suffers and tries to appropriate. The Silhouettes’ devotion to their beloved makes them dependent on him. I defend this dependence even though it is undoubtedly a form of despair. By appealing to Sartre’s account of love, I argue moreover that this love involves a recognition and appraisal of the beloved absent in the love exemplified by Fear and Trembling’s knight of infinite resignation.