bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence, predictability, and outcomes of systemic venous congestion following a fluid challenge in initially fluid-tolerant preload-responders after cardiac surgery: a pilot trial
Authors: Morosanu Bianca , Balan Cosmin , Boros Cristian , Dazzi Federico , Wong Adrian , Corradi Francesco , Bubenek-Turconi Serban-Ion 
Published: October 2024

Abstract
Background
Fluid administration has traditionally focused on preload responsiveness (PR). However, preventing fluid intolerance, particularly due to systemic venous congestion (VC), is equally important. This study evaluated the incidence and predictability of VC following a 7 ml/kg crystalloid infusion in fluid-tolerant preload-responders and its association with adverse outcomes.

Methods
This single-center, prospective, observational study (May 2023–July 2024) included 40 consecutive patients who were mechanically ventilated within 6 h of intensive care unit (ICU) admission after elective open-heart surgery and had acute circulatory failure. Patients were eligible if they were both fluid-tolerant and preload-responsive. PR was defined as a ≥ 12% increase in left-ventricular outflow tract velocity time integral (LVOT-VTI) 1 min after a passive leg raising (PLR) test. VC was defined by a portal vein pulsatility index (PVPI) ≥ 50%. Patients received a 7 ml/kg Ringer’s Lactate infusion over 10 min. The primary outcome was the incidence of VC 2 min post-infusion (early-VC). Secondary outcomes included VC at 20 min, the incidence of acute kidney injury (AKI) and severe AKI at 7 days, and ICU length of stay (LOS).

Results
45% of patients developed early-VC, with VC persisting in only 5% at 20 min. One-third of patients developed AKI, with 17.5% progressing to severe AKI. The median ICU LOS was 4 days. Patients with early-VC had significantly higher central venous pressure, lower mean perfusion pressure, worse baseline right ventricular function, and a higher incidence of severe AKI. While LVOT-VTI returned to baseline by 20 min in both groups, PVPI remained elevated in early-VC patients (p < 0.001). The LVOT-VTI versus PVPI regression line showed similar slopes (p = 0.755) but different intercepts (p < 0.001), indicating that, despite fluid tolerance and PR at baseline, early-VC patients had reduced right ventricular diastolic reserve (RVDR). Post-PLR PVPI predicted early-VC with an area under the curve of 0.998, using a threshold of 44.3% (p < 0.001).

Conclusion
Post-PLR PVPI effectively predicts fluid-induced early-VC in fluid-tolerant preload-responders, identifying those with poor RVDR. Its use can guide fluid management in cardiac surgery patients, helping to prevent unnecessary fluid administration and associated complications.
Trial Registration: NCT06440772. Registered 30 May 2024. Retrospectively registered.
Golgiphagy: a novel selective autophagy to the fore
Authors: Chen Yifei , Wu Yihui , Tian Xianyan , Shao Genbao , Lin Qiong , Sun Aiqin 
Published: October 2024

AbstractThe Golgi apparatus is the central hub of the cellular endocrine pathway and plays a crucial role in processing, transporting, and sorting proteins and lipids. Simultaneously, it is a highly dynamic organelle susceptible to degradation or fragmentation under various physiological or pathological conditions, potentially contributing to the development of numerous human diseases. Autophagy serves as a vital pathway for eukaryotes to manage intracellular and extracellular stress and maintain homeostasis by targeting damaged or redundant organelles for removal. Recent research has revealed that autophagy mechanisms can specifically degrade Golgi components, known as Golgiphagy. This review summarizes recent findings on Golgiphagy while also addressing unanswered questions regarding its mechanisms and regulation, aiming to advance our understanding of the role of Golgiphagy in human disease.
Insights into the length and breadth of methodologies harnessed to study human telomeres
Authors: Coulter Tiernan , Hill Claire , McKnight Amy Jayne 
Published: October 2024

AbstractTelomeres are protective structures at the end of eukaryotic chromosomes that are strongly implicated in ageing and ill health. They attrition upon every cellular reproductive cycle. Evidence suggests that short telomeres trigger DNA damage responses that lead to cellular senescence. Accurate methods for measuring telomeres are required to fully investigate the roles that shortening telomeres play in the biology of disease and human ageing. The last two decades have brought forth several techniques that are used for measuring telomeres. This editorial highlights strengths and limitations of traditional and emerging techniques, guiding researchers to choose the most appropriate methodology for their research needs. These methods include Quantitative Polymerase Chain Reaction (qPCR), Omega qPCR (Ω-qPCR), Terminal Restriction Fragment analysis (TRF), Single Telomere Absolute-length Rapid (STAR) assays, Single TElomere Length Analysis (STELA), TElomere Shortest Length Assays (TESLA), Telomere Combing Assays (TCA), and Long-Read Telomere Sequencing. Challenges include replicating telomere measurement within and across cohorts, measuring the length of telomeres on individual chromosomes, and standardised reporting for publications. Areas of current and future focus have been highlighted, with recent methodical advancements, such as long-read sequencing, providing significant scope to study telomeres at an individual chromosome level.
Controls of low injectivity caused by interaction of reservoir and clogging processes in a sedimentary geothermal aquifer (Mezőberény, Hungary)
Authors: Markó Ábel , Brehme Maren , Pedretti Daniele , Zimmermann Günter , Huenges Ernst 
Published: October 2024

AbstractLow injectivity is often experienced in geothermal doublets installed in sandstone reservoirs. This even led to a shutdown of the Mezőberény (Hungary) geothermal site. An on-site campaign was carried out in January 2021 to prepare a stimulation aiming to enhance the transmissivity of the sedimentary reservoir and the near-wellbore zone of this site. Previous studies have concluded that insufficient injectivity may be linked to a high skin effect in the near well-bore zone and pore clogging in combination with the low net sandstone content of the fluvio-deltaic reservoir. A chemical soft stimulation based on the injection of hydrochloric acid (HCl) was successfully used to unclog and recover the well injectivity. Despite such empirical evidence, the geochemical mechanisms leading to both, detrimental formation of clogging and the HCl-driven transmissivity restoration, have not yet been elucidated. This work presents the results of a novel analysis aiming at (a) predicting the dominant type of clogging forming in the near-well bore zone; (b) quantifying the drop in hydraulic conductivity as clogging occurs; and (c) supporting the optimization of the HCl dosage during the chemical soft stimulation. The study is supported by new experimental datasets never presented before from the Mezőberény site and a geochemical model set-up simulating the main mechanisms involved in the clogging and unclogging processes. It is concluded that the biofilm formation was the dominant, while the precipitation of calcite and amorphous ferrihydrite—later reduced to magnetite by microbes—was the secondary clogging mechanism: In the long-term (yearly scale) simulating the hydraulic conductivity showed a decline with forming scales; therefore, biofilm was presumably responsible for the experienced rapid (1 month) clogging. When modelling the chemical stimulation, the estimated amount of precipitated minerals was dissolved already with 2.5 mol of HCl per liter of water (~ 10 m/m%). Therefore, the 20 m/m% of HCl chosen during the field campaign might had a beneficial effect dissolving the potentially higher amount of scaling and/or the carbonate minerals of the matrix near the wellbore. Overall, it is concluded that the chemical and the microbial analyses together with the geochemical model were critical to tailor the remediation attempts and to propose further development or reconstruction of the surface system before going into operation to prevent recurrent impairments. Our findings highlight the importance of interactions of various clogging mechanisms with each other as well as with the reservoir processes and provide approaches to tackle the issue of injectivity drop by characterizing and quantifying their effects.
Multi-hazard risk assessment analysis in La Palma: an approach for risk mitigation
Authors: Martín-Raya Nerea , Díaz-Pacheco Jaime , López-Díez Abel 
Published: October 2024

Abstract
Background
La Palma, like many regions of the world, may be affected by various hazards, with different levels of susceptibility, which have a greater or lesser impact on society. In this context, a multi-risk approached model capable of estimating the total risk faced by the territory is required, considering the different hazards to which it is exposed (floods, earthquakes, volcanic eruptions and landslides) and the different conditions of vulnerability of both the population and the exposed assets.

Methods
The methodology employed in this study has been referred to as "multilayer single hazard", which consists of the aggregation or superposition of hazards located in the same space. As usual, the identification of the individual hazard of each one of them was carried out using heterogeneous methods, which is why an index is proposed to homogenize the hazards and make them comparable with each other. On the other hand, the model also integrates a prototype to evaluate vulnerability from a multiple perspective (vulnerability by hazard) and shows the aggregated information in maps at a medium representation scale (definition of 250 m).

Results
The risk integration results show that 5% of the island is at high risk levels. Simultaneously, the south of the island is presented as the area that shows the highest risk by combining a high hazard, especially related to volcanic hazards, and a high exposure, as it is home to a significant volume of population.

Conclusions
The proposed methodology is also interesting because it can be replicated in other areas of study.
“Like two peas in a pod?” Homogamous personalities, education, and union dissolution
Authors: Hofmann Elias , Krapf Sandra 
Published: October 2024

AbstractThis paper examines the association between the level of similarity in the “Big Five” personality traits of the partners in different-sex couples and their risk of union dissolution. Prior research has mainly focused on homogamy in socio-economic, demographic, and cultural characteristics, such as age, education, employment, and religion. The few studies on the effects of homogamy in the personalities of the partners on separation find mixed results. We extend on this by analysing the moderating effect of education. Using data from the German Socio-Economic Panel (SOEP) for the 2005–2019 period, we follow 3958 coresidential couples and observe 534 separations. Personality is measured via the “Big Five” personality traits. We estimate discrete-time event history models for union dissolution. In addition to reporting the main effects, we calculate interactions between personality and the level of education of the partners. Our results indicate that greater dissimilarity with regard to the personality trait “openness” is associated with a higher probability to separate. However, analysing interaction effects reveals that this is relevant mainly among medium educated men. Moreover, persons with high education seem to be less likely to separate if they are dissimilar from their partner in their level of “extraversion”. These findings suggest that relationship dynamics differ across educational groups.
The integrated Mexican nation-state building in the 20th century
Authors: Zhang Qingren 
Published: October 2024

AbstractSince gaining independence in the early 19th century, Mexico embarked on a path toward building a nation-state. In criticizing the evolutionism that promotes white supremacy, Mexican society has embarked on a journey of introspection, delving into the essence of its indigenous national character. Following the Mexican Revolution at the turn of the 20th century, a nationalism advocating for integration was firmly established. The Institutional Revolutionary Party (PRI) government incorporated the principles of national integration into the broader building of the nation-state. On the one hand, it affirmed the crucial role of indigenous peoples and their cultures, tracing the origins of the Mexican nation-state back to these roots. On the other hand, the promotion of unified education, coupled with the implementation of a land-centered state corporatist system, aimed to achieve the integration and governance of Indigenous peoples, thereby incorporating them into the nation-building process and ultimately facilitating the integration of the nation-state. While the PRI government’s integration efforts led to tendencies towards marginalization and assimilation of indigenous peoples, alongside insufficient attention to their rights and political participation, it fundamentally changed the historically unrecognized status of indigenous communities since the colonial era. These efforts primarily addressed the issues that had challenged Mexican social development concerning indigenous peoples since the colonial era, contributing to maintaining national stability over an extended period.
Ultrasound-guided cryoablation of early breast cancer: safety, technical efficacy, patients’ satisfaction, and outcome prediction with MRI/CEM: a pilot case-control study
Authors: Galati Francesca , Pasculli Marcella , Maroncelli Roberto , Rizzo Veronica , Moffa Giuliana , Cerbelli Bruna , d’Amati Giulia , Catalano Carlo , Pediconi Federica 
Published: October 2024

Abstract
Background
This pilot prospective study aimed to evaluate ultrasound-guided cryoablation of breast cancer (BC) by assessing: (i) technical efficacy as the presence of necrosis in surgical specimens and rate of complete tumor ablation; (ii) safety as incidence and severity of complications; and (iii) patients’ satisfaction using a dedicated questionnaire. In addition, (iv) we tested the capability of magnetic resonance imaging (MRI) or contrast-enhanced mammography (CEM) to predict cryoablation efficacy.

Methods
From 07/2022 to 01/2023, we enrolled 20 patients with early-stage BC scheduled for breast surgery. Ten of them, with a cryo-feasible cancer location, were sent to cryoablation (cryo-group) and ten to routine surgical practice (control group). Both groups underwent surgery and were asked to answer a satisfaction questionnaire.

Results
Of eleven patients screened for cryoablation, only one refused to be treated at another hospital (acceptance rate 10/11, 91%). Surgery was quadrantectomy in 19 cases and mastectomy in 1. In the cryo-group, the procedure was completed and steatonecrosis was observed in 10/10 cases, with complete tumor ablation in nine of them. The post-procedural status was evaluated with MRI in five patients, with CEM in four patients, and with ultrasound in one patient who refused MRI and CEM. MRI or CEM correctly predicted complete cryoablation in eight patients and incomplete cryoablation in one patient. Patients in both groups did not have serious complications and responded positively to satisfaction questionnaires.

Conclusion
Ultrasound-guided cryoablation of early-stage BC is well accepted by patients, effective, and safe. MRI and CEM were able to predict the procedure's technical efficacy.

Trial registration
https://clinicaltrials.gov/study/NCT05727813 updated February 14, 2023.

Relevance statement
Our pilot study showed that ultrasound-guided cryoablation is a promising nonsurgical alternative for treating early-stage BC.

Key Points

Ultrasound-guided cryoablation was effective and safe in early BC patients.

The procedure was well-tolerated, with low morbidity and high patient satisfaction.

MRI and CEM predicted cryoablation efficacy, in accordance with histopathologic findings.

Cryoablation can be considered a potential alternative to surgery in selected patients.

Graphical Abstract
Quantitative ultrasound assessment of fatty infiltration of the rotator cuff muscles using backscatter coefficient
Authors: Toto-Brocchi Marco , Wu Yuanshan , Jerban Saeed , Han Aiguo , Andre Michael , Shah Sameer B. , Chang Eric Y. 
Published: October 2024

Abstract
Background
To prospectively evaluate ultrasound backscatter coefficients (BSCs) of the supraspinatus and infraspinatus muscles and compare with Goutallier classification on magnetic resonance imaging (MRI).

Methods
Fifty-six participants had shoulder MRI exams and ultrasound exams of the supraspinatus and infraspinatus muscles. Goutallier MRI grades were determined and BSCs were measured. Group means were compared and the strength of relationships between the measures were determined. Using binarized Goutallier groups (0–2 versus 3–4), areas under the receiver operating characteristic curves (AUROCs) were calculated. The nearest integer cutoff value was determined using Youden’s index.

Results
BSC values were significantly different among most Goutallier grades for the supraspinatus and infraspinatus muscles (both p < 0.001). Strong correlations were found between the BSC values and Goutallier grades for the supraspinatus (τb = 0.72, p < 0.001) and infraspinatus (τb = 0.79, p < 0.001) muscles. BSC showed excellent performance for classification of the binarized groups (0–2 versus 3–4) for both supraspinatus (AUROC = 0.98, p < 0.0001) and infraspinatus (AUROC = 0.98, p < 0.0001) muscles. Using a cutoff BSC value of −17 dB, sensitivity, specificity, and accuracy for severe fatty infiltration were 87.0%, 90.0%, and 87.5% for the supraspinatus muscle, and 93.6%, 87.5%, and 92.7% for the infraspinatus muscle.

Conclusion
BSC can be applied to the rotator cuff muscles for assessment of fatty infiltration. For both the supraspinatus and infraspinatus muscles, BSC values significantly increased with higher Goutallier grades and showed strong performance in distinguishing low versus high Goutallier grades.

Relevance statement
Fatty infiltration of the rotator cuff muscles can be quantified using BSC values, which are higher with increasing Goutallier grades.

Key Points

Ultrasound BSC measurements are reliable for the quantification of muscle fatty infiltration.

BCS values increased with higher Goutallier MRI grades.

BCS values demonstrated high performance for distinguishing muscle fatty infiltration groups.

Graphical Abstract
The impact of sex differences on stroke risk factors and 3-month outcomes in patients receiving thrombolytic therapy for acute ischemic stroke
Authors: Khedr Eman M. , Abo-Elfetoh Noha , Hasan Asmaa M. , Nasreldein Ahmed , Haridy Nourelhoda A. 
Published: October 2024

Abstract
Background
Acute ischemic stroke (AIS) is a major public health issue, and women have a disproportionate share of stroke-related disability and mortality, which is poorly understood. This study aimed to determine the effect of sex differences on AIS treated by thrombolysis using recombinant tissue plasminogen activator (rTPA). The study included 134 AIS patients eligible for rTPA. Risk factors, clinical presentation, thrombolysis response, complications, and outcomes were recorded. The outcomes were measured using the National Institute of Health Stroke Scale (NIHSS) and the modified Rankin Scale (mRS) 3 months after stroke. The incidence of intracranial haemorrhage was also determined.

Results
Among 134 AIS patients treated with rTPA (59% male, 41% female), females had higher rates of hypertension (67.3% versus 49.4%, p = 0.040) and atrial fibrillation (AF) (12.7% versus 2.5%, p = 0.020), while smoking was more prevalent in males (31.6% versus 0%, p < 0.0001). Cardioembolism was more frequent in females than in males (23.6% versus 6.3%, p = 0.004). Females also had shorter onset-to-door (110.7 ± 63 versus 131.1 ± 50.2 min, p = 0.035) and onset-to-needle times (151.6 ± 66.5 versus 173.7 ± 50.6 min, p = 0.046). Both sexes showed significant improvements in NIHSS (males: 11.58 ± 3.7 to 6.05 ± 5.6; females: 11.64 ± 4.7 to 6.9 ± 5.9; p < 0.0001) and mRS scores (males: 3.34 ± 1.05 to 2.03 ± 2; females: 3.53 ± 1 to 2.02 ± 2; p < 0.0001) over 3 months, with no significant differences between sexes. Predictors of poor outcome (mRS 3–6) differed: diabetes mellitus (DM) (OR 7.79, p = 0.002) and longer door-to-needle time (OR 1.04, p = 0.008) for males, and hemorrhage (OR 9.41, p = 0.048) for females. Hemorrhage predicted mortality in males (OR 27.08, p < 0.0001), while AF was associated with increased mortality in females (OR 8.06, p = 0.024).

Conclusions
This study revealed sex-specific differences in AIS risk factors, aetiology, and rTPA treatment timelines, although post-treatment improvements were comparable between sexes. Notably, outcome predictors and mortality factors differed by sex. These findings emphasize the need for sex-specific considerations in AIS management and risk assessment.
RNA silencing is activated by N gene-mediated hypersensitive response and plays a key role in local and systemic virus resistance
Authors: Li Wenjing , Yu Ru , Liu Wenjuan , Zhang Hong , Ren Chengyang , Zhang Yan , Ma Haoran , Zhu Changxiang , Liu Hongmei 
Published: October 2024

AbstractIn plants, recognition between resistance gene (R) and virus induces a local hypersensitive response (HR), which is accompanied by systemic acquired resistance (SAR). The dominant resistance gene N in tobacco confers resistance to tobacco mosaic virus (TMV) at both locally inoculated tissues and systemically infected tissues. However, the mechanisms underlying HR- and SAR-mediated viral inhibition are not fully revealed. In this study, we find that Nicotiana glutinosa RNA-binding protein (NgRBP) is an RNA silencing suppressor which enhances TMV-triggered HR. Stronger HR could result in stronger local and systemic RNA silencing as well as SAR. Enhanced RNA silencing in the systemically infected leaves induced by the NgRBP gene is compromised by transient expression of NahG. These results indicate that RNA silencing is activated by HR and plays a crucial role in local and systemic virus resistance. Our results reveal a crosstalk between N gene-mediated virus resistance and RNA silencing which would deepen our understanding of the established HR and SAR models.
Outcomes of left atrial appendage closure versus oral anticoagulant therapy in patients with atrial fibrillation: an updated meta-analysis of randomized control trials
Authors: Pramana Ketut Angga Aditya Putra , Cahyani Ni Gusti Ayu Made Sintya Dwi , Pintaningrum Yusra , Rahmat Basuki 
Published: October 2024

Abstract
Background
The purpose of this study is to compare the clinical results of Left Atrial Appendage Closure (LAAC) and oral anticoagulation (OAC) in individuals with AF.

Methods
For randomized controlled trials (RCTs) comparing the clinical results of OAC to LAAC in patients with atrial fibrillation (AF), we searched PubMed, ScienceDirect, and Cochrane. The included publications were subjected to meta-analyses using Review Manager v5.4.

Results
In comparison to OAC, LAAC was linked with a decreased incidence of all stroke (OR 0.68; 95% CI 0.55–0.84; p = 0.0004). LAAC was also linked to a decreased risk of hemorrhagic stroke (OR 0.20, 95% CI 0.07–0.55; p = 0.002). There is no statistically significant difference between the two groups in terms of ischemic stroke (OR 1.05; 95% CI 0.59–1.84; p = 0.88) or systemic embolization (OR 1.02; 95% CI 0.42–2.46; p = 0.97).

Conclusions
According to our meta-analysis, the LAAC was less likely than the OAC to have a complete or hemorrhagic stroke. For the two groups, however, there was no difference in the risk of ischemic stroke or systemic embolization.