bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐potassium volcanic rocks from the Okinawa Trough: Implications for a cryptic potassium‐rich and DUPAL‐like source
Authors: Zhang Yuxiang , Zeng Zhigang , Li Xiaohui , Yin Xuebo , Wang Xiaoyuan , Chen Shuai 
Published: August 2017

Major element, trace element, and Sr–Nd–Pb isotopic compositions of high‐potassium calc‐alkaline rhyolites (the T6 rhyolites) collected from the western slope of the middle Okinawa Trough (MOT) are reported in this article. These high‐potassium rhyolites have similar major and trace elemental compositions as Type 2 rhyolites from the MOT, except that the former contain higher concentrations of K2O, Th, U, and Rb and lower concentrations of Sr and Eu. Experimental data indicate that the T6 rhyolites cannot be sourced from low‐ to mid‐potassium MOT rocks by either fractional crystallization or partial melting processes but are instead derived from a cryptic potassium‐rich source beneath the Okinawa Trough (OT). Considering that high‐potassium volcanic rocks are distributed throughout the East China Sea Shelf, the Ryukyu Arc, and Kyushu Island, the high‐potassium OT rocks could represent an important connection between the OT and its adjacent regions. Compared to volcanic rocks from the MOT axial zone, the T6 rhyolites have more depleted Sr–Nd isotopic compositions (87Sr/86Sr = 0.7035–0.7036, 143Nd/144Nd = 0.51291–0.51297) but much more enriched compositions of 207Pb (207Pb/204Pb = 15.60–15.63) and 208Pb (208Pb/204Pb = 38.45–38.57), thus recording a significant DUPAL‐like signature. These isotopic anomalies cannot be explained by the incorporation of subducting sediments or crustal contamination but instead imply the presence of a DUPAL‐like mantle source beneath the OT. The decrease of the DUPAL‐like anomaly from the T6 rhyolites to the MOT axial zone volcanic rocks is consistent with the injection of an normal mid‐ocean ridge basalt (N‐MORB) mantle source, such as asthenospheric material, into pre‐existing DUPAL‐like mantle during back‐arc extension.
Functional neuroimaging after severe anoxic brain injury in children may reveal preserved, yet covert, cognitive function
Authors: Owen Adrian M. 
Published: August 2017

AbstractA growing body of evidence has confirmed that, after severe brain injury in adults, motoric and task‐dependent factors that are essential for reliable communication, frequently interfere with an accurate assessment of cognitive status. In the current study, resting state functional magnetic resonance imaging (fMRI) in children who have sustained an anoxic brain injury following a near drowning incident suggests a similar pattern; preserved cognition amidst severe motoric impairment that effectively precludes accurate clinical diagnosis at the bedside. Hum Brain Mapp 38:4832–4833, 2017. © 2017 Wiley Periodicals, Inc.
Estradiol rapidly increases GluA2‐mushroom spines and decreases GluA2‐filopodia spines in hippocampus CA1
Authors: Avila Jorge A. , Alliger Amber A. , Carvajal Brigett , Zanca Roseanna M. , Serrano Peter A. , Luine Victoria N. 
Published: August 2017

AbstractHippocampal dendritic spine density rapidly increases following estradiol (E2) treatment, but the types of spines and trafficking of synaptic markers have received little investigation. We assessed rapid effects of E2 over time on the density of four spine types (stubby, filopodial, long thin, and mushroom) and trafficking of AMPA receptor subunit GluA2 and PSD95 on tertiary, apical dendrites in CA1. Castrated male rats received 20 μg kg−1 of E2 or vehicle and were sacrificed 30 or 120 min later. Images of Golgi‐Cox impregnated and PSD95/GluA2 stained dendrites were captured under the confocal microscope and quantified with IMARIS‐XT. Stubby and filopodial spine densities did not change following treatment. Long‐thin spines significantly decreased at 30 min while mushroom spines significantly increased at 120 min. GluA2, PSD95, and GluA2/PSD95 colocalization levels in stubby or long thin spines did not change, but filopodial spines had significantly reduced GluA2 levels at 30 min. Mushroom spines showed significantly increased levels for GluA2, PSD95 and GluA2/PSD95 colocalization at 120 min. Because GluA2 is important for memory consolidation, current results present novel data suggesting that trafficking of GluA2 to mushroom spines provides one mechanism contributing to estradiol's ability to enhance learning and memory by the PI3 signaling pathway.
Comparison of regional cerebral blood flow in Parkinson's disease with depression and major depression
Authors: Kim Young‐Do , Jeong Hyeonseok S. , Kim Yong‐Duk 
Published: August 2017

AbstractDiagnosis of depression in Parkinson's disease (PD) patients is complicated due to the overlapping symptoms of the two disorders. We investigated regional cerebral blood flow (rCBF) in patients with only major depression (MD) and PD patients with (PDMD) and without MD (PDNMD) using 99mTc hexamethylpropyleneamine oxime single photon emission computed tomography (HMPAO‐SPECT). A total of 103 patients (38 PDMD, 46 PDNMD, and 19 MD patients) underwent brain HMPAO‐SPECT scans. Voxel‐wise whole‐brain analysis was conducted to compare rCBF of the PDMD group with that of the PDNMD and MD groups. The scores of the Geriatric Depression Scale (GDS) and Neuropsychiatric Inventory (NPI) were significantly higher in the PDMD group than in the PDNMD group. The PDMD group showed significant hypoperfusion in the subcallosal cortex than the PDNMD group. The MD group showed significant hypoperfusion in the brainstem and inferomedial frontal region compared with the PDMD group. Our findings suggest that dysfunction of the inferomedial frontal cortex may be involved in the pathogenesis of depression in PD patients.
Fuzzy generalized fast marching method for 3D segmentation of brain structures
Authors: Baghdadi Mohamed , Benamrane Nacéra , Sais Lakhdar 
Published: August 2017

AbstractThe aim of this work is to develop a new model for segmentation of brain structures in medical brain MR images. Brain segmentation is a challenging task due to the complex anatomical structure of brain structures as well as intensity nonuniformity, partial volume effects and noise. Generally the structures of interest are of relatively complicated size and have significant shape variations, the structures boundaries may be blurry or even missing, and the surrounding background is full of irrelevant edges. Segmentation methods based on fuzzy models have been developed to overcome the uncertainty caused by these effects. In this study, we propose a robust and accurate brain structures segmentation method based on a combination of fuzzy model and deformable model. Our method breaks up into two great parts. Initially, a preliminary stage allows to construct the various information maps, in particular a fuzzy map, used as a principal information source, constructed using the Fuzzy C‐means method (FCM). Then, a deformable model implemented with the generalized fast marching method (GFMM), evolves toward the structure to be segmented, under the action of a normal force defined from these information maps. In this sense, we used a powerful evolution function based on a fuzzy model, adapted for brain structures. Two extensions of our general method are presented in this work. The first extension concerns the addition of an edge map to the fuzzy model and the use of some rules adapted to the segmentation process. The second extension consists of the use of several models evolving simultaneously to segment several structures. Extensive experiments are conducted on both simulated and real brain MRI datasets. Our proposed approach shows promising and achieves significant improvements with respect to several state‐of‐the‐art methods and with the three practical segmentation techniques widely used in neuroimaging studies, namely SPM, FSL, and Freesurfer.
In vitro genotoxicity of airborne Ni‐NP in air–liquid interface
Authors: Latvala Siiri , Vare Daniel , Karlsson Hanna L. , Elihn Karine 
Published: August 2017

AbstractStudies using advanced toxicological methods enabling in vitro conditions that are more realistic are currently needed for understanding the risks of pulmonary exposure to airborne nanoparticles. Owing to the carcinogenicity of certain nickel compounds, the increased production of nickel nanoparticles (Ni‐NPs) raises occupational safety concerns. The aim of this study was to investigate the genotoxicity of airborne Ni‐NPs using a recently developed air–liquid interface exposure system. The wild‐type Chinese hamster lung fibroblast cell line (V79) was used and cytotoxicity, DNA damage and mutagenicity were studied by testing colony forming efficiency, alkaline DNA unwinding and HPRT mutation assays, respectively. Additionally, co‐exposure to a PARP‐1 inhibitor was performed to test possible involvement of base excision repair (BER) in repair of Ni‐induced DNA damage. The results showed that cell viability was reduced significantly (to 45% and 46%) after 48 hours Ni‐NP exposure at concentrations of 0.15 and 0.32 μg cm−2. DNA damage was significantly increased after Ni‐NP exposure in the presence of the BER inhibitor indicating that Ni‐NP‐induced DNA damages are subsequently repaired by BER. Furthermore, there was no increased HPRT mutation frequency following Ni‐NP exposure. In conclusion, this study shows that Ni‐NP treatment of lung fibroblasts in an air–liquid interface system that mimics real‐life exposure, results in increased DNA strand breaks and reduced cellular viability. These DNA lesions were repaired with BER in an error‐free manner without resulting in mutations. This study also underlines the importance of appropriate quantification of the actual exposure concentrations during air–liquid interface exposure studies.
A novel endpoint for the assessment of chemotherapy‐induced peripheral neuropathy in rodents: biomechanical properties of peripheral nerve
Authors: Liu Chang‐Ning , Berryman Edwin , Zakur David , Shoieb Ahmed M. , Pardo Ingrid D. , Boucher Magalie , Somps Chris J. , Bagi Chedo M. , Cook Jon C. 
Published: August 2017

AbstractChemotherapy‐induced peripheral neuropathy (CiPN) is a frequent adverse effect in patients and a leading safety consideration in oncology drug development. Although behavioral assessment and microscopic examination of the nerves and dorsal root ganglia can be incorporated into toxicity studies to assess CiPN risk, more sensitive and less labor‐intensive endpoints are often lacking. In this study, rats and mice administered vincristine (75 μg kg−1 day−1, i.p., for 10 days in rats and 100 μg kg−1 day−1, i.p., for 11 days in mice, respectively) were employed as the CiPN models. Behavioral changes were assessed during the dosing phase. At necropsy, the sural or sciatic nerve was harvested from the rats and mice, respectively, and assessed for mechanical and histopathological endpoints. It was found that the maximal load and the load/extension ratio were significantly decreased in the nerves collected from the animals dosed with vincristine compared with the vehicle‐treated animals (P < 0.05). Additionally, the gait analysis revealed that the paw print areas were significantly increased in mice (P < 0.01), but not in rats following vincristine administration. Light microscopic histopathology of the nerves and dorsal root ganglia were unaffected by vincristine administration. We concluded that ex vivo mechanical properties of the nerves is a sensitive endpoint, providing a new method to predict CiPN in rodent. Gait analysis may also be a useful tool in these pre‐clinical animal models.
The Effect of Depression, Generalized Anxiety, and Selective Serotonin Reuptake Inhibitors on Change in Bone Metabolism in Adolescents and Emerging Adults
Authors: Calarge Chadi A , Mills James A , Janz Kathleen F , Burns Trudy L , Schlechte Janet A , Coryell William H , Zemel Babette S 
Published: August 2017

ABSTRACT

The purpose of this study was to prospectively examine the independent contribution of major depressive disorder (MDD), generalized anxiety disorder (GAD), and selective serotonin reuptake inhibitors (SSRIs) use to changes in bone metabolism in older adolescents and emerging adults. Medically healthy 15- to 20-year-olds who were unmedicated or within 1 month of starting an SSRI were prospectively followed. Psychiatric functioning and medication treatment were assessed monthly. Every 4 months, trabecular and cortical volumetric bone mineral density (vBMD) at the radius and markers of bone metabolism were evaluated. Every 8 months, total body less head areal bone mineral content and lumbar spine (LS) areal BMD (aBMD) were determined. Linear mixed-effects regression analysis examined associations between bone measures on the one hand and MDD, GAD, and SSRI indices on the other. A total of 264 participants were followed for 1.51 ± 0.76 years. After adjusting for age, sex, vitamin D concentration, physical activity, lean mass or grip strength, and time in the study, MDD severity was associated with increasing LS aBMD. Similarly, SSRI use was associated with increasing LS aBMD and bone formation in female participants. In contrast, SSRI use was associated with decreasing LS aBMD in males. After accounting for depression, GAD was independently, albeit weakly, associated with increased bone mineralization. In older adolescents and emerging adults, MDD and GAD are associated with increasing bone mass, particularly in the lumbar spine and in females, whereas SSRIs are associated with increasing bone mass in females but decreasing bone mass in males. © 2017 American Society for Bone and Mineral Research.
Impact Microindentation: Consistency of Serial Measurements and Alterations in Patients With Paget's Disease of the Tibia
Authors: Malgo Frank , Hamdy Neveen AT , Papapoulos Socrates E , Appelman-Dijkstra Natasha M 
Published: August 2017

ABSTRACT

Impact microindentation (IMI) is a new technique for the in vivo measurement of tissue-level properties of cortical bone in humans. To address issues related to the proper application of IMI in clinical practice and to directly examine cortical bone properties in patients with tibia pathology, we studied 11 subjects without tibia pathology and nine patients with Paget's disease of the tibia in biochemical remission after bisphosphonate treatment. Serial indentations in the tibias of both legs were performed in all subjects by a single operator until 10 adequate measurements were obtained in each tibia. In patients without Paget's disease (7 men and 4 women; mean age, 61.9 years; range, 51 to 72 years), there was no difference in mean bone material strength index (BMSi) between the dominant and nondominant leg (82.1 ± 1.3 and 81.4 ± 1.3, respectively; p = 0.606). In each individual subject studied, sequential indentations in both legs showed no trends for higher or lower values with time. The standard deviation of unnormalized bone material strength (BMSu) was also comparable between the dominant and nondominant tibia (5.3 and 4.5, respectively; p = 0.657). In patients with Paget's disease (4 men and 5 women; mean age, 69.5 years; range, 55 to 87 years), mean BMSi of the Pagetic tibia was lower, albeit nonsignificantly, than that of the contralateral nonaffected tibia (74.7 ± 1.7 and 78.7 ± 1.3, respectively; p = 0.120). In contrast to subjects without Paget's disease, the SD of adequate BMSu values was significantly larger in the Pagetic tibia compared to that of the non-Pagetic tibia (7.6 versus 5.0, respectively, p = 0.008). These results highlight the consistency of serial IMI measurements as performed by a single operator in the presence as well as absence of tibia pathology and illustrate that the method is able to capture alterations of tissue-level cortical bone properties in patients with Paget's disease of the tibia. © 2017 The Authors.Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.
Bone marrow and adipose mesenchymal stem cells attenuate cardiac fibrosis induced by methotrexate in rats
Authors: Fikry Ebtehal Mohammad , Hassan Wedad A. , Gad Amany M. 
Published: August 2017

AbstractMesenchymal stem cells (MSCs) are an ideal adult stem cell with capacity for self‐renewal and differentiation with an extensive tissue distribution. The present study evaluates the therapeutic effects of bone marrow mesenchymal stem cells (BM‐MSCs) or adipose‐derived mesenchymal stem cells (AD‐MSCs) against the development of methotrexate (MTX)‐induced cardiac fibrosis versus dexamethasone (DEX). Rats were allocated into five groups; group 1, received normal saline orally; group 2, received MTX (14 mg/kg/week for 2 weeks); groups 3 and 4, treated once with 2 × 106 cells of MTX + BM‐MSCs and MTX + AD‐MSCs, respectively; and group 5, MTX + DEX (0.5 mg/kg, for 7 days, P.O.). MTX induced cardiac fibrosis as marked changes in oxidative biomarkers and elevation of triglyceride, cholesterol, aspartate aminotransferase, gamma‐glutamyl transferase, creatine kinase, and caspase‐3, as well as deposited collagen. These injurious effects were antagonized after treatment with MSCs. So, MSCs possessed antioxidant, antiapoptotic, as well antifibrotic effects, which will perhaps initiate them as notable prospective for the treatment of cardiac fibrosis.
Attenuation of DNA damage and mRNA gene expression in hypoxic rats using natural antioxidants
Authors: Fadda Laila Mohamed , Attia Hala A. , Al‐Rasheed Nouf Mohamed , Ali Hanaa Mahmoud , Aldossari Manal 
Published: August 2017

AbstractThis study aimed to explore the efficiency of carnosine (Cs) and/or l‐arginine (Agn) in the downregulation of apoptotic and inflammatory molecule expression and DNA damage caused hepatic injury in response to sodium nitrite (Sd)‐induced hypoxia in rats. Rats were injected with Sd; Agn or/and Cs were administrated prior to Sd intoxication. Sd significantly decreased hemoglobin concentration and Bcl‐2 mRNA expression, while increased expressions of apoptotic markers (Bax and caspase), tumor necrosis factor‐α, nuclear factor kappa B, and C‐reactive protein and the oxidative DNA damage in hepatic tissue. Moreover, administration of Agn or/and Cs exhibited a modulation of the previous parameters. However, concurrent treatment with the forementioned antioxidants modulated these levels. It was concluded that the treatment with the combination of Agn and Cs was the most effective regimen in ameliorating Sd toxicity accompanied by hypoxic stress.
Prophylactic effects of ellagic acid and rosmarinic acid on doxorubicin‐induced neurotoxicity in rats
Authors: Rizk Hanan A. , Masoud Marwa A. , Maher Omar W. 
Published: August 2017

AbstractDoxorubicin (DOX) is a chemotherapeutic agent widely used in human malignancies. Its long‐term use cause neurobiological side effects. The aim of the present study was to investigate the prophylactic effect exerted by daily administration of ellagic acid (EA) and rosmarinic acid (RA) on DOX‐induced neurotoxicity in rats. Our data showed that DOX‐induced significant elevation of brain malondialdehyde, tumor necrosis factor‐alpha (TNF‐α), inducible nitric oxide synthase (iNOS), caspase‐3, and cholinesterase associated with significant reduction in reduced glutathione, monoamines namely serotonin, dopamine, as well as norepinephrine. Concomitant administration of EA (10 mg/kg/day, p.o. for 14 days) and/or RA (75 mg/kg/day, p.o. for 14 days) with DOX significantly mitigated the neural changes induced by DOX. Meanwhile, treatment ameliorated pro‐inflammatory cytokines as TNF‐α, iNOS, and attenuated oxidative stress biomarkers as well as brain monoamines. In conclusion, EA and RA can effectively protect against DOX‐induced neurotoxicity, and the mechanisms underlying the neuroprotective effect are potentially associated with its antioxidant, anti‐inflammatory, and antiapoptotic properties.
A potential high risk for fatty liver disease was found in mice generated after assisted reproductive techniques
Authors: Gu Leilei , Zhang Jingjing , Zheng Meimei , Dong Guoying , Xu Jingyi , Zhang Wuyue , Wu Yibo , Yang Yang , Zhu Hui 
Published: August 2017

AbstractAbnormal gametogenesis and embryonic development may lead to poor health status of the offspring. The operations involved in the assisted reproductive technologies (ARTs) occur during the key stage of gametogenesis and early embryonic development. To assess the potential risk of abnormal lipid metabolism in the liver of adult ARTs offspring, two ARTs mice models derived from preimplantation genetic diagnosis (PGD group) and in vitro cultured embryos without biopsy (IVEM group) were constructed. And control mice were from in vivo naturally conceived (Normal group). The results showed that ARTs offspring had increased body weight and body fat content comparing to normal group. An increasing volume and amount of lipid droplets as well as lipid droplet fusion were found in the hepatocytes of ARTs mice, and a significantly increased liver TG content was also shown in the ARTs mice, which due to the increased TG synthesis and decreased TG transport in the liver. All the results indicated that the manipulations involved in ARTs might play an important role in the lipid accumulation of adult offspring. By analyzing the DNA methylation profiles of 7.5dpc embryos, we proposed that methylation deregulation of the genes related to liver development in ARTs embryos might contribute to the abnormal phenotype in the offspring. The study demonstrated that ARTs procedures have adverse effect on liver development which resulted in abnormal lipid metabolism and induced the potential high risk of fatty liver in adulthood.
New insight into the control of peptic ulcer by targeting the histamine H2 receptor
Authors: Singh Vijai , Gohil Nisarg , Ramírez‐García Robert 
Published: August 2017

AbstractPeptic ulcer disease is one of the major challenges in public health globally and new evidence shows that it can be controlled by targeting the histamine H2 receptor (H2R). Recently, a number of H2R antagonists have been synthesized and used to block the action of histamine on the parietal cells in the stomach and decrease the acid production. In this study, we modeled the H2R by homology modeling using the 3‐D crystal structure and this model was validated based on free energy and amino acid residues present in the allowed regions of a Ramachandran plot. We used this 3‐D model for screening of highly potent drugs using molecular docking. We found cimetidine, cimetex, and famotidine as the most potent drugs based on the binding affinity of drug‐protein interactions. We also generated a cellular network for H2R that could be useful for better understanding of cellular mechanism and drug targets. These findings provide a new insight into the development of suitable, specific, and effective anti‐ulcer drugs for a most effective treatment of ulcerous diseases.