bluuebunny/crossref_metadata_2025_split huggingface dataset:
Working Memory Performance Predicts, but Does Not Reduce, Cocaine and Cannabinoid Seeking in Adult Male Rats
Authors: Stringfield Sierra J , Kirschmann Erin K , Torregrossa Mary M 
Published: October 2024

Abstract

Background
Cognitive deficits reflecting impaired executive function are commonly associated with psychiatric disorders, including substance use. Cognitive training is proposed to improve treatment outcomes for these disorders by promoting neuroplasticity within the prefrontal cortex, enhancing executive control, and mitigating cognitive decline due to drug use. Additionally, brain derived neurotrophic factor (BDNF) can facilitate plasticity in the prefrontal cortex and reduce drug-seeking behaviors. We investigated whether working memory training could elevate BDNF levels in the prefrontal cortex and if this training would predict or protect against cocaine or cannabinoid seeking.

Methods
Adult male rats were trained to perform a “simple” or “complex” version of a delayed-match-to-sample working memory task. Rats then self-administered cocaine or the synthetic cannabinoid WIN55,212-2 and were tested for cued drug seeking during abstinence. Tissue from the prefrontal cortex and dorsal hippocampus was analyzed for BDNF protein expression.

Results
Training on the working memory task enhanced endogenous BDNF protein levels in the prelimbic prefrontal cortex but not the dorsal hippocampus. Working memory training did not impact self-administration of either drug but predicted the extent of WIN self-administration and cocaine seeking during abstinence.

Conclusions
These results suggest that working memory training promotes endogenous BDNF but does not alter drug-seeking or drug-taking behavior. However, individual differences in cognitive performance before drug exposure may predict vulnerability to future drug use.
Light and temperature signals are integrated through a phytochrome B-dependent gene regulatory network in rice
Authors: Catarino Bruno , Andrade Luís , Cordeiro André M , Carvalho Pedro , Barros Pedro M , Blázquez Miguel A , Saibo Nelson J M 
Published: October 2024

Abstract
Angiosperms are the most dominant land plant flora and have colonized most of the terrestrial habitats, thriving in different environmental conditions, among which light and temperature play a crucial role. In the eudicot Arabidopsis thaliana, light and temperature are integrated into a phytochrome B (phyB)-dependent signalling network that regulates development. However, whether this signal integration controls the development in other angiosperm lineages and whether phyB is a conserved hub of this integratory network in angiosperms is unclear. We used a combination of phylogenetic, phenotypic, and transcriptomic analyses to understand the phyB-dependent light and temperature integratory network in the monocot Oryza sativa and infer its conservation in angiosperms. Here, we showed that light and temperature co-regulate rice growth through a phyB-dependent regulatory network that shares conserved features between O. sativa and A. thaliana. Despite the conservation of the components of this regulatory network, the transcriptional regulation between the components has changed qualitatively since monocots and eudicots diverged (~192–145 million years ago). The evolutionary flexibility of this integratory network might underlie the successful adaptation of plants to diverse ecological niches. Furthermore, our findings provide promising candidate genes whose activity and expression can be fine-tuned to improve plant growth and productivity in a warming planet.
The Lost Plays of Ralph Sheldon (1623–1684)
Authors: Grinke Paul 
Published: October 2024

Abstract
This article traces the fate of an important group of early English plays in fifty-six volumes, collected by the Warwickshire antiquary Ralph Sheldon (1623-1684) and sold at a Christie’s country house sale at his home nearly a hundred years later. The sale itself in 1781 was an early example of the booksellers ‘ring’ and involved some of the major collectors of early English drama, notably David Garrick, Edmond Malone, and the Earl of Charlemont. The earlier portion of the Sheldon plays, recorded by Anthony Wood in a manuscript list, may have survived as part of the Malone bequest to the Bodleian Library. The Restoration Plays remained largely intact, in some thirty volumes, until the ill-fated sale of Lord Charlemont’s library by his descendants at Sotheby’s in 1865, discussed by Arthur and Janet Ing Freeman in The Library, VII, 23 (March 2022).
Pathological R-loops in bacteria from engineered expression of endogenous antisense RNAs whose synthesis is ordinarily terminated by Rho
Authors: Pandiyan Apuratha , Mallikarjun Jillella , Maheshwari Himanshi , Gowrishankar Jayaraman 
Published: October 2024

Abstract
In many bacteria, the essential factors Rho and NusG mediate termination of synthesis of nascent transcripts (including antisense RNAs) that are not being simultaneously translated. It has been proposed that in Rho’s absence toxic RNA–DNA hybrids (R-loops) may be generated from nascent untranslated transcripts, and genome-wide mapping studies in Escherichia coli have identified putative loci of R-loop formation from more than 100 endogenous antisense transcripts that are synthesized only in a Rho-deficient strain. Here we provide evidence that engineered expression in wild-type E. coli of several such individual antisense regions on a plasmid or the chromosome generates R-loops that, in an RNase H-modulated manner, serve to disrupt genome integrity. Rho inhibition was associated with increased prevalence of antisense R-loops also in Xanthomonas oryzae pv. oryzae and Caulobacter crescentus. Our results confirm the essential role of Rho in several bacterial genera for prevention of toxic R-loops from pervasive yet cryptic endogenous antisense transcripts. Engineered antisense R-looped regions may be useful for studies on both site-specific impediments to bacterial chromosomal replication and the mechanisms of their resolution.
The Influence of Kinesiology Tape on Posture and Breathing Mechanics in Healthy Individuals: A Randomized Trial
Authors: Lindenberg Kelly M. , Shipe Nancy K. , Kendall Michaela , King Scot , Kohlmann Matthew , McDowell Ashley , Nunley Courtney , Roberts Joshue , Naylor Lauren , Braunlich Jacob 
Published: October 2024

Background and Purpose:
Kinesiology tape (KT) has been used clinically to improve posture, mobility, and muscle strength. Limited research has investigated the effect of KT on respiratory function. The purpose of this study was to explore the effects of KT on posture and breathing in healthy individuals.

Methods:
Ninety-two adult participants were randomly assigned to a KT, sham tape (ST), or control group. Data collection occurred over 2 sessions. Baseline measurements included chest wall expansion (CWE) at the sternal angle (SA) and xiphoid process (Xi), maximal inspiratory pressure (MIP), and tragus (TWD) and acromion (AWD) to wall distances. Next, KT or ST was applied to the upper back and neck, or no tape was applied based on group assignment. Measurements were then repeated. Participants returned for a final round of measurements 48 hours later. Mixed-measures ANOVAs were used to determine the influence of tape at baseline (T0), immediately post-taping (T1), and 48 hours post-taping (T2). Post hoc analyses used the Tukey method.

Results:
Analysis showed significant differences between T0 and T1 (P = .004) and between T1 and T2 (P = .004) for AWD on the left. CWE-Xi data showed within group differences T0-T2 (P = .004) and T1-T2 (P = .004). MIP data showed within group differences between T0 and T1 (P = .001), T0 and T2 (P < .001), and T1 and T2 (P < .001).

Discussion:
KT had a limited and inconsistent impact on posture where there was a decrease in AWD on the left from T0 to T1 and then an increase from T1 to T2. No other changes in posture were found. Taping did not affect CWE at the SA. There was an evident training effect for CWE-Xi and MIP, where all groups saw increases over time.

Conclusion:
This method of KT application is not supported as an intervention for influencing posture and enhancing inspiratory breathing mechanics in healthy adults. Further investigation of KT in other populations is needed.
Exploring the prescription laws of bone flaccidity in ancient Chinese medical texts based on association rules and latent structure model
Authors: Yuan Yulu , Chu Ce , Tao Xuguang , Yang Zhen , Chen Xiangyun , Ding Wei , He Zhanzhan , Xu Yongqi , Zhang Yuxin , Zhao Peizhang , Chen Wanping , Zhao Hongxia , Wang Wenlai 
Published: October 2024

Objective:
To analyze the identification and typing of bone flaccidity and the law of medication in ancient books based on the Encyclopaedia of TCM (Traditional Chinese Medicine) using the hidden structure model.

Methods:
The formulations for bone flaccidity were searched in the Chinese Medical Code (software), based on which an Excel sheet was created to conduct frequency analysis and construct a matrix of high-frequency medications. The high-frequency medications for bone flaccidity were then analyzed using the Latent 5.0 software and a latent structure model. With SPSS Modeler and Latent 5.0 as the main analysis methods, Chinese medications with a frequency >1.00% were investigated using the latent structure model and association rules, and the medication rules in treating bone flaccidity with TCM were dissected based on the data of frequency.

Results:
A total of 223 TCM prescriptions were included, with 270 extracted TCM and a total medication frequency of 2340 times. Among them are Achyranthis Bidentatae Radix, Poria, Angelicae Sinensis Radix, Eucommiae Cortex, Rehmanniae Radix Praeparata, and Ginseng Radix Et Rhizoma. They were the high-frequency medicines for treating bone flaccidity, and the efficacy of tonifying medicinal, heat-clearing medicinal, and blood activating and stasis-resolving medicinal were high-frequency medicinal categories. Through the analysis of the evidence by formula, it was concluded that spleen-kidney yang deficiency, kidney deficiency and blood stasis, kidney yang deficiency, qi deficiency/qi stagnation and blood stasis, and liver and kidney yin deficiency were the 6 main types of bone flaccidity, which was generally in line with the etiology of bone flaccidity, which was caused by kidney yang deficiency and accompanied by malfunction of the liver and kidneys. In ancient books, the regulation of the kidney is put into an important position. In this study, 19 correlative principles for the treatment of bone flaccidity were derived, whose main combinations are Paeoniae Radix Alba - Angelicae Sinensis Radix, Eucommiae Cortex + Poria-Ginseng Radix Et Rhizoma, Dioscoreae Spongiosae Rhizoma + Cuscutae Semen-Eucommiae Cortex.

Conclusions:
The medication law of bone flaccidity in the Encyclopaedia of TCM mainly shows that it attaches importance to the regulation of tonifying the liver and kidney to strengthen the tendons and bones and emphasizes that the regulation of the 3 Zang–fu organs, namely the kidney, the liver and the spleen, can play an important role in the treatment of bone flaccidity.
Projection of visual material on postoperative delirium in patients undergoing cardiac surgery: A double blind randomized clinical trial
Authors: Méndez-Martínez Carlos , Casado-Verdejo Inés , Fernández-Fernández Jesús Antonio , Sánchez-Valdeón Leticia , Bello-Corral Laura , Méndez-Martínez Sara , Sandoval-Diez Adrián , Gómez-Salgado Juan , García-Suárez Mario , Fernández-García Daniel 
Published: October 2024

Background:
Postoperative delirium is a neurobehavioral disorder that can appear after surgery. It is characterized by an altered level of consciousness and impaired cognitive function. The aim of this study was to evaluate the impact of visual projection of images of relatives or loved ones in patients undergoing cardiac surgery in the immediate postoperative period, and its influence on the incidence and development of postoperative delirium.

Methods:
A randomized, double-blind clinical trial was designed in the immediate postoperative period of adult patients undergoing cardiac surgery. Consolidated Statement of Reporting Trials guidelines were followed. A control group (CG) and an intervention group (IG) were established. In the IG, the patients underwent a visual projection, while the usual unit treatment was carried out with the CG. Sociodemographic, anthropometric, anesthetic, and surgical variables were also recorded. The postoperative delirium assessment scale used was the confusion assessment method for diagnosing delirium in intensive care unit patients.

Results:
Information was collected from 104 patients undergoing cardiac surgery. Most of the patients included in the study were men (66.35%) and the most performed surgical intervention was aortic valve replacement (34.62%). In the CG, positive patients in postoperative delirium increased from 19.23% to 25%, while in the IG they decreased from 5.77% to 1.92%. The logistic regression analysis presents a prediction model where the variables that influence the model are gender and group membership, meaning that being female and belonging to the IG significantly reduce the presence of delirium.

Conclusion:
The projection of visual material reduced the incidence of postoperative delirium in patients undergoing cardiac surgery, although it cannot be established that it is effective as a treatment once the pathology is already established.
Clinical and pathological characteristics of gastric large cell neuroendocrine carcinoma: A report of 2 cases series and literature review
Authors: Wang Bo , Si Yuan , Dou Yan , Li Yongcai , Liu Zhongxin , Huang Chaokang , Xu Xin 
Published: October 2024

Rationale:
Gastric large cell neuroendocrine carcinoma (LCNEC) is a rare, aggressive neuroendocrine carcinoma that arises from the stomach and with high malignancy. Patients with gastric LCNEC usually have a poor prognosis. The standard treatment plan has not been established and its curative effect is poor. The present study described 2 cases diagnosed with gastric LCNEC and reviewed in depth the literature to improve our understanding more about this uncommon tumor and further to provide more experience to diagnose and treat this disease.

Patient concerns:
The present study reported 2 cases of gastric LCNEC in male patients aged 51 and 73 years old, respectively. Both patients had epigastric discomfort, pain, acid reflux and heartburn. The medical history was unremarkable.

Diagnoses:
The ulcerative lesion located at gastric was examined in the 2 patients by taking the esophagogastroduodenoscopy (EGO), computed tomography (CT) and digital gastrointestinal radiography (GI), that both were suspected gastric malignancy. Endoscopic biopsies of the tumor led to the initial diagnosis of gastric cancer. Postoperative pathological and immunohistochemical examinations of the surgical specimens confirmed that 1 case had mixed adeno-neuroendocrine carcinoma (MANEC) and the other had LCNEC.

Interventions:
Both patients underwent surgical resection and received etoposide-cisplatin combination chemotherapy following surgery.

Outcomes:
The operation was successful. Both patients had uneventful recoveries following surgery, and had been followed-up regularly. The general condition was satisfactory, and no tumor metastasis was observed at present.
A novel BCHE frameshift mutation in a Chinese woman with butyrylcholinesterase deficiency: A case report and literature review
Authors: Zeng Jiantao , Yang Dan , Dai Tao , Xia Jun , Zheng Zhaobin 
Published: October 2024

Rationale:
Congenital butyrylcholinesterase deficiency (BCHED) is a rare autosomal recessive genetic disorder caused by a pathogenic mutation in the BCHE gene. Patients with BCHED may experience prolonged apnea or even death after the application of drugs such as succinylcholine. We aimed to identify the genetic basis of disease in a patient presenting with butyrylcholinesterase deficiency in order to confirm the diagnosis, expand BCHE gene mutation spectrum, and elucidate potential genotype-phenotype associations to inform management.

Patient concerns:
A 51-year-old woman presented with “vague pain in the upper and middle abdomen.” Her serum cholinesterase level was 211 U/L (reference value 4000–13,000 U/L). Other laboratory findings were normal. Genetic analysis revealed compound heterozygous mutations in BCHE gene, which was considered pathogenic in this case.

Diagnoses:
The patient presented with low serum cholinesterase levels, which excluded common causes such as liver disease, drug toxicity, and chronic illness. Whole exon examination revealed compound heterozygous mutations in the BCHE gene; thus, the patient was diagnosed with congenital BCHED.

Interventions:
Gastroscopy without succinylcholine or mivacurium chloride was recommended. The gastroscopy results were “gastric polyps,” and gastroscopic “polypectomy” was performed. The patient was advised to avoid succinylcholine use.

Outcomes:
The patient’s serum cholinesterase level was reviewed 3 months later, and the result was 215 U/L. Double heterozygous mutations are the cause of BChE deficiency of this woman in this study, including a novel mutation NM_000055.4: c.666_669del (p.Phe223Glufs*38). A review of the literature reveals considerable variation in the hotspot variants of the BCHE gene across different populations. The Chinese population displays a higher prevalence of the silent type, which is more sensitive to anesthetics such as succinylcholine.

Lessons:
Clinical manifestations of congenital BCHED were not significant. This study avoided a potential anesthetic accident, and the novel variant enriched the BCHE gene mutation spectrum.
Successful placement of a chest wall venous infusion port via persistent left superior vena cava: A case report
Authors: Wang Ziqiang , Li Rongguo , Du Qiwei , Zhang Weijun , Wang Zhenyu 
Published: October 2024

Rationale:
Persistent left superior vena cava (PLSVC) is a rare congenital venous anomaly occurring in approximately 0.3% to 0.5% of the population. The presence of PLSVC complicates central venous catheter placement, increasing procedural risks. This case report describes the successful placement of a chest wall venous infusion port in a patient with PLSVC, offering valuable insights for managing similar cases and ensuring safer clinical outcomes.

Patient concerns:
A 51-year-old female, 3 weeks post-right breast cancer surgery, was admitted for her first adjuvant hemotherapy session. She requested the placement of a venous infusion port due to the prolonged duration of chemotherapy.

Diagnoses:
Imaging studies suggested the presence of PLSVC. Echocardiography revealed a dilated coronary sinus, and subsequent chest computed tomography and angiography confirmed the presence of PLSVC.

Interventions:
A chest wall venous infusion port was inserted via the left internal jugular vein. Due to the PLSVC, the catheter was adjusted to ensure proper placement.

Outcomes:
The patient successfully completed chemotherapy without any complications or discomfort associated with the venous port. Imaging studies, including chest X-ray and computed tomography, confirmed proper port function and catheter positioning, with no evidence of thrombosis, infection, or other related issues. The patient remained in good overall condition throughout the treatment.

Lessons:
Detailed preoperative evaluations, intraoperative imaging guidance, and postoperative follow-ups are crucial for the safe and effective management of PLSVC patients undergoing central venous catheter placement.
Lived Experience of Postpartum Women in Recovery for Opioid Use Disorder on Medication-Assisted Therapy
Authors: MacPherson Caron 
Published: October 2024

Abstract

Purpose:
The purpose of this study was to understand the lived experiences of postpartum women on medication-assisted therapy for opioid use disorder.

Study Design and Methods:
van Manen's interpretive hermeneutic phenomenological design was used. Recruitment and data collection from seven participants occurred at an outpatient office offering care for perinatal patients on medication-assisted therapy for opioid use disorder. Data collection occurred between September 2021 and March 2022 via in-person interviews using a demographic data sheet, interview guide, speech recognition software, and digital recordings. Demographic data were analyzed using descriptive statistics. Qualitative data were analyzed using precoding, coding, and thematic analysis via van Manen's interpretive hermeneutic phenomenological method.

Results:
Seven themes were identified: troubled origins, used opioids to deal with life issues, needed to be self-reliant, opioid use disorder overtook their life, the baby was a motivator to seek treatment, the need for combined medication-assisted therapy and collaborative care, and hope for a better life.

Clinical Nursing Implications:
Nurses can provide trauma-informed care, support the importance of medication-assisted therapy in recovery, incorporate a collaborative care team with expertise in postpartum opioid use disorder, continue the collaborative care team for a minimum of 1 year, and advocate for the patient's role as parent.
Holistic Nursing Care for Perinatal Loss Based on Swanson's Theory of Caring
Authors: Berry Shandeigh N. , Lazcano Shania 
Published: October 2024

Abstract

Purpose:
The purpose of this study was to inform perinatal loss care by expanding upon the five processes of Swanson's Theory of Caring through a holistic lens.

Study Design and Methods:
This study was a qualitative content analysis of 34 interview transcripts of parents' experiences with perinatal loss. A priori codes were identified using a holistic model which guided the integration of parents' holistic needs into Swanson's Theory.

Results:
Nurses can address parents' physical, psychological, social, spiritual, and cultural needs using Swanson's Theory to provide high-quality, holistic care that may foster healing. Parents were appreciative of nurses who were transparent, did not avoid talking about their baby, facilitated memorialization, and fostered bonding. Anticipating parents' needs, being emotionally present, and recognizing the meaning of the perinatal loss experienced helped parents to have a more positive loss experience.

Clinical Implications:
By incorporating holistic interventions into care guided by Swanson's Theory, nurses can empower parents within their loss experience. Interventions to create memories, foster bonding and connection, promote autonomy, and celebrate personhood may facilitate healing. It is necessary to ensure nurses have the support and resources to attend to parents' holistic needs, including education and sufficient time for care interactions. Providing education in perinatal loss and grief, particularly to new nurse graduates, is necessary to ensure nurses are equipped to provide holistic care to families experiencing perinatal loss.