bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of the 5f valence orbitals of early actinides in chemical bonding
Authors: Vitova T. , Pidchenko I. , Fellhauer D. , Bagus P. S. , Joly Y. , Pruessmann T. , Bahl S. , Gonzalez-Robles E. , Rothe J. , Altmaier M. , Denecke M. A. , Geckeis H. 
Published: July 2017

AbstractOne of the long standing debates in actinide chemistry is the level of localization and participation of the actinide 5f valence orbitals in covalent bonds across the actinide series. Here we illuminate the role of the 5f valence orbitals of uranium, neptunium and plutonium in chemical bonding using advanced spectroscopies: actinide M4,5 HR-XANES and 3d4f RIXS. Results reveal that the 5f orbitals are active in the chemical bonding for uranium and neptunium, shown by significant variations in the level of their localization evidenced in the spectra. In contrast, the 5f orbitals of plutonium appear localized and surprisingly insensitive to different bonding environments. We envisage that this report of using relative energy differences between the 5fδ/φ and 5fπ*/5fσ* orbitals as a qualitative measure of overlap-driven actinyl bond covalency will spark activity, and extend to numerous applications of RIXS and HR-XANES to gain new insights into the electronic structures of the actinide elements.
El Niño-like teleconnection increases California precipitation in response to warming
Authors: Allen Robert J. , Luptowitz Rainer 
Published: July 2017

AbstractFuture California (CA) precipitation projections, including those from the most recent Climate Model Intercomparison Project (CMIP5), remain uncertain. This uncertainty is related to several factors, including relatively large internal climate variability, model shortcomings, and because CA lies within a transition zone, where mid-latitude regions are expected to become wetter and subtropical regions drier. Here, we use a multitude of models to show CA may receive more precipitation in the future under a business-as-usual scenario. The boreal winter season-when most of the CA precipitation increase occurs-is associated with robust changes in the mean circulation reminiscent of an El Niño teleconnection. Using idealized simulations with two different models, we further show that warming of tropical Pacific sea surface temperatures accounts for these changes. Models that better simulate the observed El Niño-CA precipitation teleconnection yield larger, and more consistent increases in CA precipitation through the twenty-first century.
Tunable graphene-based hybrid plasmonic modulators for subwavelength confinement
Authors: Qu Sheng , Ma Congcong , Liu Hongxia 
Published: July 2017

AbstractElectro-optical modulators which work at the near-infrared range are significant for a variety of applications such as communication and sensing. However, currently available approaches result in rather bulky devices which suffer from low integration and can hardly operate at low power consumption levels. Graphene, an emerging advanced material, has been widely utilized due to its tunability by gating which allows one to realize active optical devices. Plasmonic waveguides, one of the most promising candidates for subwavelength optical confinement, provide a way to manipulate light on scales much smaller than the wavelength. In this paper, we combine the advantages of graphene and plasmonic waveguides and propose a tunable graphene-based hybrid plasmonic modulator (GHPM). Considering several parameters of the GHPM, the modulation depth can reach approximately 0.3 dB·μm−1at low gating voltages. Moreover, we combine GHPM with metal-insulator-metal (MIM) structure to propose another symmetrical GHPM with a modulation depth of 0.6 dB·μm−1. Our modulators which utilize the light-matter interaction tuned by electro-doped graphene are of great potential for many applications in nanophotonics.
Dynamics of snap-off and pore-filling events during two-phase fluid flow in permeable media
Authors: Singh Kamaljit , Menke Hannah , Andrew Matthew , Lin Qingyang , Rau Christoph , Blunt Martin J. , Bijeljic Branko 
Published: July 2017

AbstractUnderstanding the pore-scale dynamics of two-phase fluid flow in permeable media is important in many processes such as water infiltration in soils, oil recovery, and geo-sequestration of CO2. The two most important processes that compete during the displacement of a non-wetting fluid by a wetting fluid are pore-filling or piston-like displacement and snap-off; this latter process can lead to trapping of the non-wetting phase. We present a three-dimensional dynamic visualization study using fast synchrotron X-ray micro-tomography to provide new insights into these processes by conducting a time-resolved pore-by-pore analysis of the local curvature and capillary pressure. We show that the time-scales of interface movement and brine layer swelling leading to snap-off are several minutes, orders of magnitude slower than observed for Haines jumps in drainage. The local capillary pressure increases rapidly after snap-off as the trapped phase finds a position that is a new local energy minimum. However, the pressure change is less dramatic than that observed during drainage. We also show that the brine-oil interface jumps from pore-to-pore during imbibition at an approximately constant local capillary pressure, with an event size of the order of an average pore size, again much smaller than the large bursts seen during drainage.
Structural and Biochemical Characterization of Apicomplexan Inorganic Pyrophosphatases
Authors: Jamwal Abhishek , Yogavel Manickam , Abdin Malik. Z. , Jain Swatantra. K. , Sharma Amit 
Published: July 2017

AbstractInorganic pyrophosphatases (PPase) participate in energy cycling and they are essential for growth and survival of organisms. Here we report extensive structural and functional characterization of soluble PPases from the human parasites Plasmodium falciparum (PfPPase) and Toxoplasma gondii (TgPPase). Our results show that PfPPase is a cytosolic enzyme whose gene expression is upregulated during parasite asexual stages. Cambialistic PfPPase actively hydrolyzes linear short chain polyphosphates like PPi, polyP3 and ATP in the presence of Zn2+. A remarkable new feature of PfPPase is the low complexity asparagine-rich N-terminal region that mediates its dimerization. Deletion of N-region has an unexpected and substantial effect on the stability of PfPPase domain, resulting in aggregation and significant loss of enzyme activity. Significantly, the crystal structures of PfPPase and TgPPase reveal unusual and unprecedented dimeric organizations and provide new fundamental insights into the variety of oligomeric assemblies possible in eukaryotic inorganic PPases.
Dynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus
Authors: Moslehi Maryam , Ng Dominic C. H. , Bogoyevitch Marie A. 
Published: July 2017

AbstractDoublecortin X (DCX), known to be essential for neuronal migration and cortical layering in the developing brain, is a 40 kDa microtubule (MT)-associated protein. DCX directly interacts with MTs via its two structured doublecortin (DC) domains, but the dynamics of this association and the possible regulatory roles played by the flanking unstructured regions remain poorly defined. Here, we employ quantitative fluorescence recovery after photobleaching (FRAP) protocols in living cells to reveal that DCX shows remarkably rapid and complete exchange within the MT network but that the removal of the C-terminal region significantly slows this exchange. We further probed how MT organization or external stimuli could additionally modulate DCX exchange dynamics. MT depolymerisation (nocodazole treatment) or stabilization (taxol treatment) further enhanced DCX exchange rates, however the exchange rates for the C-terminal truncated DCX protein were resistant to the impact of taxol-induced stabilization. Furthermore, in response to a hyperosmotic stress stimulus, DCX exchange dynamics were slowed, and again the C-terminal truncated DCX protein was resistant to the stimulus. Thus, the DCX dynamically associates with MTs in living cells and its C-terminal region plays important roles in the MT-DCX association.
Gold Nanoparticles dotted Reduction Graphene Oxide Nanocomposite Based Electrochemical Aptasensor for Selective, Rapid, Sensitive and Congener-Specific PCB77 Detection
Authors: Wu Lidong , Lu Xianbo , Fu Xiaochen , Wu Lingxia , Liu Huan 
Published: July 2017

AbstractGold nanoparticles (AuNP) dotted reduction graphene oxide (RGO-AuNP) is used as a platform for an aptamer biosensor to selectively detect 3,3′4,4′-polychlorinated biphenyls (PCB77). By anchoring aptamers onto the binding sites of RGO-AuNP and making use of the synergy effect of RGO and AuNP, the RGO-AuNP based biosensor exhibits superior analytical performances to AuNP based biosensor in terms of sensitivity and repeatability. The sensitivity of RGO-AuNP based aptamers (RGO-AuNP-Ap) biosensor (226.8 μA cm−2) is nearly two times higher than that of Au based biosensors (AuNP-Ap/Au electrode, 147.2 μA cm−2). The RGO-AuNP-Ap/Au biosensor demonstrated a linear response for PCB77 concentrations between 1 pg L−1 and 10 μg L−1, with a low limit of detection (LOD) of 0.1 pg L−1. The superb LOD satisfies the exposure thresholds (uncontaminated water < 0.1 ng L−1) set out by International Agency for Research on Cancer (IARC) and the Environmental Protection Agency (EPA). The proposed biosensor can be a powerful tool for rapid, sensitive and selective detection of PCBs on site.
Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum
Authors: Borowska-Zuchowska Natalia , Hasterok Robert 
Published: July 2017

AbstractNucleolar dominance (ND), initially described as ‘differential amphiplasty’, is a phenomenon observed in some plant and animal allopolyploids and hybrids in which the selective suppression of the activity of 35S rRNA gene loci that have been inherited from one of the two or more ancestral genomes occurs. Although more than 80 years have passed since the discovery of ND, there is still a significant lack in our understanding of the mechanisms that determine this phenomenon. Here, we aimed to investigate the epigenetic status of 35S rRNA gene loci in the monocotyledonous Brachypodium hybridum, which is an allotetraploid that has resulted from a cross between B. distachyon and B. stacei. We revealed that the repressed B. stacei-inherited rDNA loci are characterised by a high level of DNA methylation. The global hypomethylation of B. hybridum nuclear DNA induced by 5-azacytidine, however, seems to be insufficient for the transcriptional reactivation of these loci, which indicates that factors other than DNA methylation are behind the suppression of B. stacei-originated loci. We also showed that the transcriptionally active and silenced fractions of rRNA genes that had been inherited from B. distachyon occupy different domains within the chromocentres adjacent to the nucleolus, depending on their epigenetic status.
Design, transform and control of optical field in discrete optical system: an example
Authors: Deng Hongchang , Yuan Yonggui , Yuan Libo 
Published: July 2017

AbstractA discrete optical system can broaden the spatial distribution of the input light through optical coupling in array waveguides, just like diffraction in continuous media. Here, we theoretically demonstrate several kinds of control methods of optical field propagation in a discrete optical system, which is composed of an Airy fiber with two perpendicular arrayed cores. A brief transform mechanism between Gaussian and Airy beam propagation in such a fiber is presented. The wavefront of the output beam from the Airy fiber is actually dependent on the phased arrayed modulation of coupling array cores. Except the optical wavelength changing, we propose two new methods, including fiber length and bending-induced refractive-index changing, to accomplish that modulation. The calculation results show that these new methods are very effective for the Airy phase modulation. By combining these methods and controlling the corresponding parameters, the Gaussian beam, the one-dimension Airy beam, and the two-dimension Airy beam can be obtained by one same Airy fiber. These methods are also generally applicable to the other discrete optical system and can be extended to generate any other types of optical beams, such as Bessel beams and Mathieu beams.
Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner
Authors: Damulewicz Milena , Loboda Agnieszka , Jozkowicz Alicja , Dulak Jozef , Pyza Elzbieta 
Published: July 2017

AbstractIn the present study, we showed that in the retina of Drosophila, the expression of the ho gene, encoding haeme oxygenase (HO), is regulated by light but only at the beginning of the day. This timing must be set by the circadian clock as light pulses applied at other time points during the day do not increase the ho mRNA level. Moreover, light-induced activation of HO does not depend on the canonical phototransduction pathway but instead involves cryptochrome and is enhanced by ultraviolet (UV) light. Interestingly, the level of DNA damage in the retina after UV exposure was inversely related to the circadian oscillation of the ho mRNA level during the night, being the highest when the HO level was low and reversed during the day. Accordingly, induction of HO by hemin was associated with low DNA damage, while inhibition of HO activity by SnPPIX aggravated the damage. Our data suggest that HO acts in the retina to decrease oxidative DNA damage in photoreceptors caused by UV-rich light in the morning.
Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
Authors: Chu Chia-Ho , Sarangadharan Indu , Regmi Abiral , Chen Yen-Wen , Hsu Chen-Pin , Chang Wen-Hsin , Lee Geng-Yen , Chyi Jen-Inn , Chen Chih-Chen , Shiesh Shu-Chu , Lee Gwo-Bin , Wang Yu-Lin 
Published: July 2017

AbstractIn this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) are used to directly detect proteins, including HIV-1 RT, CEA, NT-proBNP and CRP, in 1X PBS (with 1%BSA) or human sera. The samples do not need any dilution or washing process to reduce the ionic strength. The sensor shows high sensitivity and the detection takes only 5 minutes. The designs of the sensor, the methodology of the measurement, and the working mechanism of the sensor are discussed and investigated. A theoretical model is proposed based on the finding of the experiments. This sensor is promising for point-of-care, home healthcare, and mobile diagnostic device.
Actomyosin and vimentin cytoskeletal networks regulate nuclear shape, mechanics and chromatin organization
Authors: Keeling Michael C. , Flores Luis R. , Dodhy Asad H. , Murray Elizabeth R. , Gavara Núria 
Published: July 2017

AbstractThe regulation of nuclear state by the cytoskeleton is an important part of cellular function. Actomyosin stress fibres, microtubules and intermediate filaments have distinct and complementary roles in integrating the nucleus into its environment and influencing its mechanical state. However, the interconnectedness of cytoskeletal networks makes it difficult to dissect their individual effects on the nucleus. We use simple image analysis approaches to characterize nuclear state, estimating nuclear volume, Poisson’s ratio, apparent elastic modulus and chromatin condensation. By combining them with cytoskeletal quantification, we assess how cytoskeletal organization regulates nuclear state. We report for a number of cell types that nuclei display auxetic properties. Furthermore, stress fibres and intermediate filaments modulate the mechanical properties of the nucleus and also chromatin condensation. Conversely, nuclear volume and its gross morphology are regulated by intracellular outward pulling forces exerted by myosin. The modulation exerted by the cytoskeleton onto the nucleus results in changes that are of similar magnitude to those observed when the nucleus is altered intrinsically, inducing chromatin decondensation or cell differentiation. Our approach allows pinpointing the contribution of distinct cytoskeletal proteins to nuclear mechanical state in physio- and pathological conditions, furthering our understanding of a key aspect of cellular behaviour.
Free immunoglobulin light chain (FLC) promotes murine colitis and colitis-associated colon carcinogenesis by activating the inflammasome
Authors: Ma Junfan , Jiang Dongyang , Gong Xiaoting , Shao Wenwei , Zhu Zhu , Xu Weiyan , Qiu Xiaoyan 
Published: July 2017

AbstractNumerous studies have demonstrated that free Ig light chain (FLC), a novel inflammation mediator, participates in many inflammatory diseases by activating mast cells and extending the survival of neutrophils. However, it remains unclear whether FLC is involved in colitis and colitis-associated colon carcinogenesis (CAC). In this study, we found a significant increase in FLC in murine models of DSS (Dextran Sulfate Sodium Salt)-induced colitis and CAC compared to controls. Peptide F991, a functional blocker of FLC, significantly attenuated colitis progression, which included abrogating the development of diarrhea and tumor burden, elevating survival rate, greatly reducing the infiltration of inflammatory cells (such as ROS+active neutrophils), especially reducing tumorigenesis in CAC. Furthermore, we demonstrated that F991 inhibited the activation of the inflammasome by reducing the expression of cleaved caspase-1 and the maturation of IL-1β and IL-18. Altogether, our findings demonstrate that FLC can promote the pathogenesis of colitis and CAC and may be used as novel biomarker for the diagnosis of inflammatory bowel disease. Additionally, F991 may become a potential therapeutic option for colitis or colorectal cancer.
PSMA expression by microvasculature of thyroid tumors – Potential implications for PSMA theranostics
Authors: Bychkov Andrey , Vutrapongwatana Usanee , Tepmongkol Supatporn , Keelawat Somboon 
Published: July 2017

AbstractProstate-specific membrane antigen (PSMA) is overexpressed in prostate cancer epithelium, making it a promising target for molecular imaging and therapy. Recently, several studies found unexpected PSMA radiotracer uptake by thyroid tumors, including radioiodine-refractory (RAIR) cancers. PSMA expression was reported in tumor-associated endothelium of various malignancies, however it has not been systematically addressed in thyroid tumors. We found that PSMA was frequently expressed in microvessels of thyroid tumors (120/267), but not in benign thyroid tissue. PSMA expression in neovasculature was highly irregular ranging from 19% in benign tumors to over 50% in thyroid cancer. Such heterogeneity was not directly attributed to endothelial cell proliferation as confirmed by immunostaining with proliferation-associated endothelial marker CD105. PSMA expression was associated with tumor size (p = 0.02) and vascular invasion in follicular carcinoma (p = 0.03), but not with other baseline histological, and clinical parameters. Significant translational implication is that RAIR tumors and high-grade cancers maintain high level of PSMA expression, and can be targeted by PSMA ligand radiopharmaceuticals. Our study predicts several pitfalls potentially associated with PSMA imaging of the thyroid, such as low expression in oncocytic tumors, absence of organ specificity, and PSMA-positivity in dendritic cells of chronic thyroiditis, which is described for the first time.
TRIM21 is critical for survival of Toxoplasma gondii infection and localises to GBP-positive parasite vacuoles
Authors: Foltz Clémence , Napolitano Anna , Khan Rabia , Clough Barbara , Hirst Elizabeth M. , Frickel Eva-Maria 
Published: July 2017

AbstractInterferon gamma (IFNγ) is the major proinflammatory cytokine conferring resistance to the intracellular vacuolar pathogenToxoplasma gondiiby inducing the destruction of the parasitophorous vacuole (PV). We previously identified TRIM21 as an IFNγ-driven E3 ubiquitin ligase mediating the deposition of ubiquitin around pathogen inclusions. Here, we show that TRIM21 knockout mice were highly susceptible toToxoplasmainfection, exhibiting decreased levels of serum inflammatory cytokines and higher parasite burden in the peritoneum and brain. We demonstrate that IFNγ drives recruitment of TRIM21 to GBP1-positiveToxoplasmavacuoles, leading to Lys63-linked ubiquitination of the vacuole and restriction of parasite early replication without interfering with vacuolar disruption. As seenin vivo, TRIM21 impacted the secretion of inflammatory cytokines. This study identifies TRIM21 as a previously unknown modulator ofToxoplasma gondiiresistancein vivothereby extending host innate immune recognition of eukaryotic pathogens to include E3 ubiquitin ligases.
Temporal profiles for measuring threshold of random lasers pumped by ns pulses
Authors: Shi Xiaoyu , Chang Qing , Tong Junhua , Feng Yunjie , Wang Zhaona , Liu Dahe 
Published: July 2017

AbstractThe working threshold is an important parameter to assess the performance of cavity-free random lasers. Here, the temporal profile measurement is proposed as an alternative method to determine the thresholds of the surface plasmon based random lasers pumped by ns pulses based on analyzing the delay time (tDelay) and rising time (tR) of the emission signal. The obvious and slight inflection points of the curves of tDelay and tR varying with the pump power density are observed as indicators for the thresholds of random lasing and for the transition of lasing mode, respectively. The proposed method supplies consistent values to those supplied by traditional methods in frequency-domain for the random systems with different gain length. The demonstrated temporal profile approaches are free from the spectrometers and may be as a candidate for measuring the threshold of random lasers in ultrafast optics, nonlinear optics and bio-compatible optoelectronic probes.
Multi-photon absorption enhancement by dual-wavelength double-pulse laser irradiation for efficient dicing of sapphire wafers
Authors: Gedvilas Mindaugas , Mikšys Justinas , Berzinš Jonas , Stankevič Valdemar , Račiukaitis Gediminas 
Published: July 2017

AbstractThe evidence of multi-photon absorption enhancement by the dual-wavelength double-pulse laser irradiation in transparent sapphire was demonstrated experimentally and explained theoretically for the first time. Two collinearly combined laser beams with the wavelengths of 1064 nm and 355 nm, inter-pulse delay of 0.1 ns, and pulse duration of 10 ps were used to induce intra-volume modifications in sapphire. The theoretical prediction of using a particular orientation angle of 15 degrees of the half-wave plate for the most efficient absorption of laser irradiation is in good agreement with the experimental data. The new innovative effect of multi-photon absorption enhancement by dual-wavelength double-pulse irradiation allowed utilisation of the laser energy up to four times more efficiently for initiation of internal modifications in sapphire. The new absorption enhancement effect has been used for efficient intra-volume dicing and singulation of transparent sapphire wafers. The dicing speed of 150 mm/s was achieved for the 430 μm thick sapphire wafer by using the laser power of 6.8 W at the repetition rate of 100 kHz. This method opens new opportunities for the manufacturers of the GaN-based light-emitting diodes by fast and precise separation of sapphire substrates.