bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brazilian melon landraces resistant to Podosphaera xanthii are unique germplasm resources
Authors: Nunes E.W.L.P. , Esteras C. , Ricarte A.O. , Martínez‐Perez E. , Gómez‐Guillamón M.L. , Nunes G.H.S. , Picó M.B. 
Published: June 2017

Podosphaera xanthii is the most important causal agent of powdery mildew in melon, a crop ranked within the most economically important species worldwide. The best strategy to face this fungus disease, which causes important production losses, is the development of genetically resistant cultivars. Genetic breeding programmes require sources of resistance, and a few ones have been reported in melon, mostly in Momordica and Acidulus horticultural groups. However, the existence of many races that reduces the durability of the resistance makes necessary to find new resistant genotypes with different genetic backgrounds.In this work, Brazilian germplasm, together with a set of Indian landraces, and the COMAV's (Institute for the Conservation and Breeding of Agricultural Biodiversity) melon core collection, representing the whole variability of the species, were assessed for resistance against some common races in Spain and Brazil and genotyped with a 123‐SNP (single nucleotide polymorphisms) genotyping platform to study the molecular relationships of the resistant accessions. In the first experiment, carried out in Valencia (Spain) in 2013, seventy‐nine melon accessions were evaluated using artificial inoculation. Five accessions selected as resistant were also evaluated against races 1, 3, and 5 in Mossoró (Brazil, 2015) and against race 3.5 in Valencia (2016) under greenhouse conditions, and under four field conditions in Brazil. The accessions, AL‐1, BA‐3, CE‐3, and RN‐2, within the Brazilian collection, presented resistance against all the races of P. xanthii assayed in all conditions tested. AL‐1, CE‐3 and RN‐2 were molecularly more similar to wild agrestis and Acidulus melons from Asia and Africa, while BA‐3 grouped with Momordica types. Molecular analysis also confirmed that these new Brazilian sources of resistance differ from those previously reported, constituting interesting materials to encourage genetic breeding programmes, especially in Brazil and Spain.
Multiple Dimensions of Bureaucratic Discrimination: Evidence from German Welfare Offices
Authors: Hemker Johannes , Rink Anselm 
Published: June 2017

AbstractA growing experimental literature uses response rates to fictional requests to measure discrimination against ethnic minorities. This article argues that restricting attention to response rates can lead to faulty inferences about substantive discrimination depending on how response dummies are correlated with other response characteristics. We illustrate the relevance of this problem by means of a conjoint experiment among all German welfare offices, in which we randomly varied five traits and designed requests to allow for a substantive coding of response quality. We find that response rates are statistically indistinguishable across treatment conditions. However, putative non‐Germans receive responses of significantly lower quality, potentially deterring them from applying for benefits. We also find observational evidence suggesting that discrimination is more pronounced in welfare offices run by local governments than in those embedded in the national bureaucracy. We discuss implications for the study of equality in the public sphere.
But Some of Us Are Broke: Race, Gender, and the Neoliberalization of the Academy
Authors: Navarro Tami 
Published: June 2017

ABSTRACTAnthropologists have paid increasing attention to neoliberalism in our research, yet we have been less willing to apply this lens to our own academic positioning and the ways these roles are shaped by privatization and market models. Lives and livelihoods in the American academy are increasingly determined by neoliberalism, and it is vital that we be both reflexive about and engaged around our positions within this project. This article explores the divides that separate various forms of academic labor (secure versus nonsecure, contract, etc.) as well as the raced and gendered implications of these tracks—in particular, the stark ways in which neoliberal transformations negatively affect women of color in the academy. [neoliberalism, academy, race, gender, contingent labor]
Astroglial vesicular network: evolutionary trends, physiology and pathophysiology
Authors: Zorec R. , Parpura V. , Verkhratsky A. 
Published: June 2017

AbstractIntracellular organelles, including secretory vesicles, emerged when eukaryotic cells evolved some 3 billion years ago. The primordial organelles that evolved in Archaea were similar to endolysosomes, which developed, arguably, for specific metabolic tasks, including uptake, metabolic processing, storage and disposal of molecules. In comparison with prokaryotes, cell volume of eukaryotes increased by several orders of magnitude and vesicle traffic emerged to allow for communication between distant intracellular locations. Lysosomes, first described in 1955, a prominent intermediate of endo‐ and exocytotic pathways, operate virtually in all eukaryotic cells including astroglia, the most heterogeneous type of homeostatic glia in the central nervous system. Astrocytes support neuronal network activity in particular through elaborated secretion, based on a complex intracellular vesicle network dynamics. Deranged homeostasis underlies disease and astroglial vesicle traffic contributes to the pathophysiology of neurodegenerative (Alzheimer's disease, Huntington's disease), neurodevelopmental diseases (intellectual deficiency, Rett's disease) and neuroinfectious (Zika virus) disorders. This review addresses astroglial cell‐autonomous vesicular traffic network, as well as its into primary and secondary vesicular network defects in diseases, and considers this network as a target for developing new therapies for neurological conditions.
Archipelagic genes: Medical travel as a creative response to limitations and remoteness in the Maldives
Authors: Knoll Eva‐Maria 
Published: June 2017

AbstractMedical travel has become a self‐evident and intrinsic part of the medical landscape in the Republic of Maldives. This article raises the question of how exactly this has emerged as part of today's reality. The analysis describes the unfolding of a pronounced Maldivian medical travel culture by focusing on a particularly pressing health issue. The population of the Maldives has to face the world's highest prevalence of beta‐thalassaemia, a genetic disorder affecting the body's ability to create red blood cells. The country's 300:1 sea to land ratio, however, with its small population of 340 000 scattered over 200 islands is a challenging context for the curative and preventive measures in thalassaemia governance. The article follows the trail of thalassaemia‐related intra‐archipelagic and extra‐archipelagic medical travel of patients and clients requesting a blood transfusion, iron monitoring, bone marrow transplantation and prenatal diagnostic services. It discusses medical travel organised as a biosocial group activity, financed by insurance and religious bodies while interacting with the migration of health professionals.
Protein and modified vaccinia virus Ankara-based influenza virus nucleoprotein vaccines are differentially immunogenic in BALB/c mice
Authors: Altenburg A F , Magnusson S E , Bosman F , Stertman L , de Vries R D , Rimmelzwaan G F 
Published: June 2017

Summary
Because of the high variability of seasonal influenza viruses and the eminent threat of influenza viruses with pandemic potential, there is great interest in the development of vaccines that induce broadly protective immunity. Most probably, broadly protective influenza vaccines are based on conserved proteins, such as nucleoprotein (NP). NP is a vaccine target of interest as it has been shown to induce cross-reactive antibody and T cell responses. Here we tested and compared various NP-based vaccine preparations for their capacity to induce humoral and cellular immune responses to influenza virus NP. The immunogenicity of protein-based vaccine preparations with Matrix-M™ adjuvant as well as recombinant viral vaccine vector modified Vaccinia virus Ankara (MVA) expressing the influenza virus NP gene, with or without modifications that aim at optimization of CD8+ T cell responses, was addressed in BALB/c mice. Addition of Matrix-M™ adjuvant to NP wild-type protein-based vaccines significantly improved T cell responses. Furthermore, recombinant MVA expressing the influenza virus NP induced strong antibody and CD8+ T cell responses, which could not be improved further by modifications of NP to increase antigen processing and presentation.
Predicting and Assessing Progress in the Restoration of Ecosystems
Authors: Sinclair A.R.E. , Pech Roger P. , Fryxell John M. , McCann Kevin , Byrom Andrea E. , Savory C. John , Brashares Justin , Arthur Anthony D. , Catling Peter C. , Triska Maggie D. , Craig Michael D. , Sinclair Tim J.E. , McLaren Jennie R. , Turkington Roy , Beyers Rene L. , Harrower William L. 
Published: June 2017

AbstractRestoration of degraded landscapes has become necessary to reverse the pervasive threats from human exploitation. Restoration requires first the monitoring of progress toward any chosen goals to determine their resilience and persistence, and second to conduct in a comparable adjacent area but with less human impact the restoration of trophic structures and ecosystem processes to act as reference systems (controls) with which we compare the viability of the chosen goal. We present here the rationale and a method for predicting the trajectory of restoration and assessing its progress toward a predetermined state, the endpoint, using a restoration index. This assessment of restoration requires that we know when a predetermined endpoint has been achieved and whether the envisioned community of species and their interactions can be restored. The restoration index can use species’ presence or density, and the rate of change of ecosystem processes. The index applies to trophic levels, functional groups, successional stages, alternative states, and novel ecosystems. Also, our method allows measurement of the resilience of ecosystems to disturbance, a desired property for conservation and management. We provide global examples to illustrate these points.
Bringing complexity and convergence governance to consumer policy
Authors: McGregor Sue L. T. 
Published: June 2017

AbstractConsumer policy is already being shaped by a combination of governance models. This position paper argues that complexity‐oriented convergence models are a timely addition. Modern day consumer policy is characterized as interactive and integrative, replete with shifting boundaries and coalitions and evolving roles for each of state, market and society. This paper focused on governance in the consumer policy arena, arguing that this process needs to acknowledge and reconcile complexity. After describing the basic tenets of complexity theory, two characteristics of contemporary tri‐sector interaction (i.e., sector blurring and sector distortion) were discussed. These boundary characteristics necessitate the need for approaches that can accommodate complexity during consumer policy governance. Three examples of the latter were profiled: sector convergence, network governance and cross‐sector governance. These conceptualizations accommodate the dynamics, complexity and emergence of contemporary consumer policy governance.
Beyond topology: coevolution of structure and flux in metabolic networks
Authors: Morrison E. S. , Badyaev A. V. 
Published: June 2017

AbstractInteractions between the structure of a metabolic network and its functional properties underlie its evolutionary diversification, but the mechanism by which such interactions arise remains elusive. Particularly unclear is whether metabolic fluxes that determine the concentrations of compounds produced by a metabolic network, are causally linked to a network's structure or emerge independently of it. A direct empirical study of populations where both structural and functional properties vary among individuals’ metabolic networks is required to establish whether changes in structure affect the distribution of metabolic flux. In a population of house finches (Haemorhous mexicanus), we reconstructed full carotenoid metabolic networks for 442 individuals and uncovered 11 structural variants of this network with different compounds and reactions. We examined the consequences of this structural diversity for the concentrations of plumage‐bound carotenoids produced by flux in these networks. We found that concentrations of metabolically derived, but not dietary carotenoids, depended on network structure. Flux was partitioned similarly among compounds in individuals of the same network structure: within each network, compound concentrations were closely correlated. The highest among‐individual variation in flux occurred in networks with the strongest among‐compound correlations, suggesting that changes in the magnitude, but not the distribution of flux, underlie individual differences in compound concentrations on a static network structure. These findings indicate that the distribution of flux in carotenoid metabolism closely follows network structure. Thus, evolutionary diversification and local adaptations in carotenoid metabolism may depend more on the gain or loss of enzymatic reactions than on changes in flux within a network structure.
What hospitals need to know about guidelines—A mixed‐method analysis of guideline implementation in Dutch hospitals
Authors: Blume Louise H. K. , van Weert Nico J. H. W. , Busari Jamiu O. , Stoopendaal Annemiek M. V. , Delnoij Diana M. J. 
Published: June 2017

AbstractRationale, aims and objectivesThis study provides insight into how Dutch hospitals ensure that guidelines are used in practice and identifies what key messages other hospitals can learn from existing practices. We examine current practices in handling compliance and, therefore, focus on hospitals that reported that they do not experience problems in the implementation of guidelines.MethodA survey of Dutch hospital boards and 9 semistructured interviews were conducted with a purposive sample of 3 hospitals. Interviews were held with 3 representatives of each hospital, specifically, with a member of the board of directors, a member of the executive medical staff, and the manager of the quality and safety department.ResultsHospitals find guidelines necessary and useful. Hospitals have the power to improve implementation if boards of directors and medical staff are committed, intrinsically motivated, cooperate with each other, and use guidelines pragmatically. Even then, they prioritize guidelines, as resources are scarce. Despite their good work, all hospitals in this study appeared to struggle to adhere to guidelines.ConclusionsIf hospitals experience problems with guideline implementation, they tend to focus more on external expectations, leading to defensive behaviour. Hospitals that do not experience implementation problems focus more on integrating guidelines into their own policies.
Maintenance diets following rapid weight loss in obstructive sleep apnea: a pilot 1‐year clinical trial
Authors: Cayanan Elizabeth A. , Marshall Nathaniel S. , Hoyos Camilla M. , Phillips Craig L. , Serinel Yasmina , Wong Keith K. H. , Yee Brendon J. , Grunstein Ronald R. 
Published: June 2017

SummaryVery low energy diets (VLED) appear to be the most efficacious dietary‐based obesity reduction treatments in obstructive sleep apnea (OSA); however, effective weight loss maintenance strategies remain untested in this condition. Our study aimed to assess the feasibility, tolerability and efficacy of two common maintenance diets during a 10‐month follow‐up period after rapid weight loss using a 2‐month VLED. In this two‐arm, single‐centre, open‐label pilot trial, obese adult OSA patients received a 2‐month VLED before being allocated to either the Australian Guide to Healthy Eating diet (AGHE) or a low glycaemic index high‐protein diet (LGHP). Outcomes were measured at 0, 2 and 12 months. We recruited 44 patients [113.1 ± 19.5 kg, body mass index (BMI): 37.2 ± 5.6 kg m−2, 49.3 ± 9.2 years, 12 females]. Twenty‐four patients were on continuous positive airway pressure (CPAP) or mandibular advancement splint (MAS) therapy for OSA. Forty‐two patients completed the VLED. The primary outcome of waist circumference was reduced by 10.6 cm at 2 months [95% confidence interval (CI): 9.2–12.1], and patients lost 12.9 kg in total weight (95% CI: 11.2–14.6). There were small but statistically significant regains in waist circumference between 2 and 12 months [AGHE = 3.5 cm (1.3–5.6) and LGHP = 2.8 cm (0.6–5.0]. Other outcomes followed a similar pattern of change. After weight loss with a 2‐month VLED in obese patients with OSA, a structured weight loss maintenance programme incorporating commonly used diets was feasible, tolerable and efficacious for 10 months. This programme may be deployed easily within sleep clinics; however, future research should first test its translation within general clinical practice.
The influence of rapid growth on sodium salicylate pharmacokinetics in male turkeys
Authors: Poźniak B. , Motykiewicz‐Pers K. , Grabowski T. , Świtała M. 
Published: June 2017

The aim of this study was to assess the influence of growth on the pharmacokinetics of sodium salicylate (SS) in male turkeys. SS was administered intravenously at a dose of 50 mg/kg. Plasma drug concentrations were assessed by high‐performance liquid chromatography, and pharmacokinetic parameters were calculated by noncompartmental analysis. As the age increased from 6 to 13 weeks (body weight increase from 2.35 to 9.43 kg), median body clearance decreased from 1.34 to 0.87 ml/min/kg. This caused a significant increase in the median mean residence time from 3.42 to 4.44 hr. Elimination phase proved to be biphasic and two elimination half‐lives (T1/2el) were distinguished. Whereas T1/2el1 was found to increase with age by 128%, T1/2el2 represented a later but faster and less age‐dependent phase of elimination (increase by 56% in the respective groups). Volume of distribution decreased with age. These effects may lead to different therapeutic response to SS in turkeys of different age and body weights.
Effects of aspirin dose escalation on platelet function and urinary thromboxane and prostacyclin levels in normal dogs
Authors: McLewee N. , Archer T. , Wills R. , Mackin A. , Thomason J. 
Published: June 2017

Established “low” aspirin dosages inconsistently inhibit platelet function in dogs. Higher aspirin dosages consistently inhibit platelet function, but are associated with adverse effects. The objectives of this study were to use an escalation in dosage to determine the lowest aspirin dosage that consistently inhibited platelet function without inhibiting prostacyclin synthesis. Eight dogs were treated with five aspirin dosages: 0.5 mg/kg q24h, 1 mg/kg q24h, 2 mg/kg q24h, 4 mg/kg q24h and 10 mg/kg q12h for 7 days. Utilizing aggregometry and a whole‐blood platelet function analyzer (PFA‐100), platelet function was evaluated before and after treatment. Urine 11‐dehydro‐thromboxane‐B2 (11‐dTXB2) and 6‐keto‐prostaglandin‐F1α (6‐keto‐PGF1α), were measured. Compared to pretreatment, there were significant post‐treatment decreases in the maximum aggregometry amplitude and increases in the PFA‐100 closure times for all dosages expect 0.5 mg/kg q24h. There was no difference in amplitude or closure time among the 2 mg/kg q24h, 4 mg/kg q24h, and 10 mg/kg q12h dosages. Compared to pretreatment values, there was a significant decrease in urinary 11‐dTXB2‐to‐creatinine and 6‐keto‐PGF1α‐to‐creatinine ratios, but there was no dose‐dependent decrease for either metabolite. An aspirin dosage of 2 mg/kg q24h consistently inhibits platelet function without decreasing prostacyclin synthesis significantly more than lower aspirin dosages.
Genomic footprints of adaptation in a cooperatively breeding tropical bird across a vegetation gradient
Authors: Termignoni‐García Flavia , Jaramillo‐Correa Juan P. , Chablé‐Santos Juan , Liu Mark , Shultz Allison J. , Edwards Scott V. , Escalante‐Pliego Patricia 
Published: June 2017

AbstractIdentifying the genetic basis of phenotypic variation and its relationship with the environment is key to understanding how local adaptations evolve. Such patterns are especially interesting among populations distributed across habitat gradients, where genetic structure can be driven by isolation by distance (IBD) and/or isolation by environment (IBE). Here, we used variation in ~1,600 high‐qualitySNPs derived from paired‐end sequencing of double‐digest restriction site‐associatedDNA(ddRAD‐Seq) to test hypotheses related toIBDandIBEin the Yucatan jay (Cyanocorax yucatanicus), a tropical bird endemic to the Yucatán Peninsula. This peninsula is characterized by a precipitation and vegetation gradient—from dry to evergreen tropical forests—that is associated with morphological variation in this species. We found a moderate level of nucleotide diversity (π = .008) and little evidence for genetic differentiation among vegetation types. Analyses of neutral and putatively adaptiveSNPs (identified by complementary genome‐scan approaches) indicate thatIBDis the most reliable explanation to account for frequency distribution of the former, whileIBEhas to be invoked to explain those of the later. These results suggest that selective factors acting along a vegetation gradient can promote local adaptation in the presence of gene flow in a vagile, nonmigratory and geographically restricted species. The putative candidateSNPs identified here are located within or linked to a variety of genes that represent ideal targets for future genomic surveys.
Experimental test of genetic rescue in isolated populations of brook trout
Authors: Robinson Zachary L. , Coombs Jason A. , Hudy Mark , Nislow Keith H. , Letcher Benjamin H. , Whiteley Andrew R. 
Published: June 2017

AbstractGenetic rescue is an increasingly considered conservation measure to address genetic erosion associated with habitat loss and fragmentation. The resulting gene flow from facilitating migration may improve fitness and adaptive potential, but is not without risks (e.g., outbreeding depression). Here, we conducted a test of genetic rescue by translocating ten (five of each sex) brook trout (Salvelinus fontinalis) from a single source to four nearby and isolated stream populations. To control for the demographic contribution of translocated individuals, ten resident individuals (five of each sex) were removed from each recipient population. Prior to the introduction of translocated individuals, the two smallest above‐barrier populations had substantially lower genetic diversity, and all populations had reduced effective number of breeders relative to adjacent below‐barrier populations. In the first reproductive bout following translocation, 31 of 40 (78%) translocated individuals reproduced successfully. Translocated individuals contributed to more families than expected under random mating and generally produced larger full‐sibling families. We observed relatively high (>20%) introgression in three of the four recipient populations. The translocations increased genetic diversity of recipient populations by 45% in allelic richness and 25% in expected heterozygosity. Additionally, strong evidence of hybrid vigour was observed through significantly larger body sizes of hybrid offspring relative to resident offspring in all recipient populations. Continued monitoring of these populations will test for negative fitness effects beyond the first generation. However, these results provide much‐needed experimental data to inform the potential effectiveness of genetic rescue‐motivated translocations.