bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the Methods of Teaching English as a Foreign Language to Students with Autism Spectrum Disorder
Authors: Zohoorian Zahra , Matin Sadr Nader , Mohammadi-Nezhad Tayyebe , Sadr Farbod Matin 
Published: September 2024

Introduction: Teaching foreign languages, such as English, as an international language, to children with autism spectrum disorder (ASD) has been considered by the researchers of language instruction, language learning, and teachers of children with special needs. The population of children diagnosed with autism is rapidly growing and they must have similar opportunities for enhancing life skills, education, successful interactions, and universal communicative skills.
Materials and Methods: This study reviews and discusses the findings of past studies that investigated effective ways and efficient methods for teaching these children English as a foreign language.
Results: Although there has been restricted literature throughout the world, the reviewed studies demonstrated improvement of English language learning by suitable strategies and various methods, including pictures representing, humanoid robots, Montessori-oriented methods, applied behavior analysis (ABA), cognitive method, the picture exchange communication system (PECS), well-designed educational environments, mixed methods and strategies, speech therapy, media, total physical response (TPR), and individualized education plan (IEP).
Conclusion: This narrative review encourages various interventions and integrated methods for teaching the English language to autistic children, considering their differences from diverse aspects.
Investigating the Relationship Between Kinesiophobia, Catastrophizing, Pain Intensity, Disability, and Gait Performance in Chronic Neck Pain
Authors: Ery Mersad , Saadat Maryam , Hessam Masumeh , Mehravar Mohammad , Amirabadi Negar 
Published: September 2024

Introduction: There is little evidence regarding the relationship between gait performance and psychological factors in people with chronic neck pain. This study evaluates gait performance in patients with neck pain and explores the relationship between gait performance and kinesiophobia, pain catastrophizing, pain intensity, and disability.
Materials and Methods: A cross-sectional study was conducted on 34 patients with chronic neck pain and 29 age- and sex-matched controls were recruited for this study. The participants performed timed up-and-go (TUG), and 10-m walk tests (TMW) with and without head-turning tests. The associations between clinical gait tests, kinesiophobia (Tampa scale of kinesiophobia (TSK), pain catastrophizing scale, pain intensity (visual analog scale), and disability (neck disability index) were assessed.
Results: People with neck pain had significant differences in the TUG, and TMW with and without head-turning tests compared to controls (P<0.01). Kinesiophobia and pain catastrophism were significantly correlated with TMW tests (r range=0.45 to 0.71, and 0.40 to 0.47, respectively). Pain intensity and disability were not correlated with gait tests.
Conclusion: The gait performance, as represented by TUG and TMWs test scores, altered in patients with chronic neck pain in comparison controls. Fear of motion and pain catastrophizing thoughts correlated with clinical gait test scores.
Investigating the Effects of Manual Therapy and Pelvic Floor Training on Erectile Dysfunction: A Case Study
Authors: Safavi Mahdi Afshar , Niknam Hoda , Okhovatian Farshad , Roostayi Mohammad Mohsen 
Published: September 2024

Introduction: Erectile dysfunction (ED) is a common issue in men, often associated with various factors, such as obesity, diabetes, and more. This case study examines the impact of friction massage combined with pelvic floor exercises on ED in three patients, linked to prostatic adenectomy, aging, and diabetes.
Case Description: Patients followed a one-month regimen of pelvic floor exercises and techniques. The technique’s progress was monitored via video calls twice a week. Exercises lasted for 10 min daily, along with a 10-min massage. We assessed improvement using the international index of erectile function 15 (IIEF15) questionnaire and erection hardness score (EHS), as well as ultrasound scans.
Results: Following treatment, all three patients showed increased EHS and IIEF15 15 scores. Muscular thickness increased in the ischiocavernosus and bulbospongiosus muscles, while the cavernosal artery exhibited higher systolic velocity and lower diastolic velocity, although maximum blood flow velocity in the posterior vein decreased. Treatment had the most significant impact on the adenectomy patient, followed by the elderly patient and then the diabetic patient.
Conclusion: Combining friction massage and pelvic floor exercises led to improved erection- related indicators and increased patient satisfaction.
Step-by-step: A clinical pathway for stepped care management of fear of cancer recurrence– results of a three round online Delphi consensus process with Australian health professionals and researchers
Authors: Smith Allan 'Ben' , Girgis Afaf , Taylor Natalie , Pearce Alison , Liu Jia , Shepherd Heather L. , Wu Verena S. , Garvey Gail , Kirsten Laura , Zakhary Iman , Ee Carolyn , Ewald Daniel , Miller Annie , Shaw Joanne 
Published: September 2024

Abstract

Purpose
Fear of cancer recurrence (FCR) is not routinely addressed in clinical practice, meaning many cancer survivors forego effective interventions. We established expert consensus on a clinical pathway to help health professionals identify and manage FCR.
Methods
Australian health professionals and researchers working with adult cancer survivors participated in a 3-round Delphi study promoted via oncology professional bodies and social media. The Round 1 online survey presented 38 items regarding FCR screening, triage, assessment, referral, and stepped care, based on a literature review, related pathways/guidelines, and expert input. Participants rated how representative of best-practice items were on a 5-point scale (strongly disagree – strongly agree), with optional qualitative feedback. Consensus was defined as ≥80% of participants strongly/agreeing with items. Items not reaching consensus were re-presented in two subsequent rounds with new items, derived from content analysis of qualitative feedback.
Results
From 94 participants in Round 1 (89% health professionals) 26/38 (68%) items reached consensus. By round 3, 35/38 (92%) items, including 8 new items, reached consensus. Routine FCR screening and triage conversations, and stepped care management were endorsed. However, timing of FCR screening/triage did not reach consensus.
Conclusions
This world-first FCR clinical pathway incorporating contemporary evidence and expert opinion recommends routine screening and triage to stepped care management of FCR. Some pathway components, such as screening or triage timing, may need tailoring for different contexts.
Implications for Cancer Survivors
Implementation of the pathway could aid routine identification and management of FCR, reducing its burden on cancer survivors and the healthcare system.
First detection of V410L kdr mutation in Aedes aegypti populations of Argentina and its correlation with the toxicological evidence
Authors: Gonzalez Paula V. , Loureiro Aline C. , Gómez-Bravo Andrea , Castillo Paola , Espinosa Manuel , Gil José F. , Martins Ademir J , Harburguer Laura V. 
Published: September 2024

Abstract

BACKGROUND
Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika and chikungunya viruses in many parts of the world impacting millions of people worldwide each year. Insecticide-based interventions have been effective in controlling Aedes mosquito populations for several years, but in recent times, resistance to these compounds has developed, posing a global threat to the control of this mosquito.
METHODS
Ovitraps were used to collect A. aegypti eggs in the cities of Tartagal and San Ramón de la Nueva Orán (Salta), Puerto Iguazú (Misiones) and Clorinda (Formosa). WHO impregnated papers with the discriminating concentration (DC) of permethrin, 5X, 10X and pirimiphos methyl were used for the toxicological bioassays. We also genotyped each sample for the three kdr single nucleotide polymorphisms (SNP): V410L, V1016I and F1534C in individual TaqMan qPCR reactions.
RESULTS
All investigated A. aegypti populations were highly resistant to permethrin, as the mortality percentage with the permethrin 10XDC remained below 98%. However, all populations were 100% susceptible to pirimiphos-methyl. Kdr genotyping demonstrated the presence of the V410L mutation for the first time in Argentina in all the populations studied. A prevalence of the triple mutant genotype (LL + II + CC) was observed in the northeastern cities of Clorinda (83.3%) and Puerto Iguazú (55.6%).
CONCLUSIONS
This study demonstrates for the first time the presence and intensity of resistance to permethrin in different populations from Argentina, and correlates the observed phenotype with the presence of kdr mutations (genotype).
The intelligent Diabetes TelemonitoRing Using decision Support to treat patients on insulin Therapy (DiaTRUST) trial: study protocol for a randomized controlled trial
Authors: Thomsen Camilla H. N. , Nørlev Jannie D. , Hangaard Stine , Jensen Morten H. , Hejlesen Ole , Kristensen Sika N. S. , Aradóttir Tinna B. , Kaas Anne , Vestergaard Peter , Kronborg Thomas 
Published: September 2024

Abstract

Background
Diabetes affects 10.5% of adults globally, with type 2 diabetes accounting for 90–95% of cases. Achieving optimal glycemic control is crucial yet challenging, particularly with insulin therapy, where 30–50% of patients fail to meet treatment targets. Telemedicine can improve diabetes management but generates vast amounts of data, burdening healthcare providers. Integrating clinical decision support tools into telemonitoring systems may enhance care efficiency and glycemic control.
Methods
The trial is a six-month, three-arm, open-label randomized controlled study conducted at Steno Diabetes Center North Denmark, aiming to enroll 51 participants with type 2 diabetes on insulin therapy. Participants will be divided in a 3:1:1 ratio into telemonitoring with decision support, telemonitoring without decision support, and usual care groups. The study employs connected insulin pens, continuous glucose monitors (CGMs), and activity trackers to enable telemonitoring. Outcomes measured include CGM time in range, HbA1c, hypoglycemia and hyperglycemia incidents, total daily insulin dose, body weight, treatment satisfaction, and adherence.
Discussion
Telemonitoring with decision support has the potential to revolutionize diabetes management by offering personalized treatment suggestions, thereby reducing the burden on healthcare providers and improving patient outcomes. This study will provide valuable insights into the effectiveness of such an approach in achieving glycemic control in people with type 2 diabetes on insulin therapy. By evaluating both clinical outcomes and patient and healthcare providers’ satisfaction, the study aims to contribute to the development of efficient, scalable telehealth solutions for diabetes care.
Trial Registration:
The trial is registered with ClinicalTrials.gov (NCT06185296) on December 14, 2023.
CD137L promotes immune surveillance in melanoma via HLTF regulation
Authors: Liu Hong , Liang Long , Zhu Lin , Li Xin , Zhang Guan Xiong , Kuang Xinwei , Hung Mien-Chie , Liu Jing , Chen Xiang 
Published: September 2024

Abstract

Immune checkpoint blockers (ICBs) have shown considerable effect in a number of malignancies. However, only limited patients were responsive to ICBs. Therefore, it is critical to explore novel therapeutic target. Herein, by integrating multi-omics data, ICB treating melanoma patient cohort, we identified that tumor CD137L is positively correlated with efficacy of PD-1 blockade in melanoma patient cohorts. Functionally, we showed that induction of CD137L in cancer cells significantly enhanced anti-tumor immunity by facilitated CD8+ T cell survival both in vivo and in vitro. Mechanistically, we found that helicase-like transcription factor (HLTF) functioned as the key transcriptional regulator controlling CD137L expression via phosphorylation of serine at position 398. Therapeutically, we identified a FDA-approved drug, AICAR (acadesine), functioned as a CD137L specific agonist, showed combination effect with PD-1 blockade or CTLA4 blockade. Taken together, our study reveals HLTF-mediated regulatory mechanism controlling CD137L expression, potential predictor for assessing the efficacy of anti-PD-1 therapy in clinic, develop a novel combination therapy to enhance the efficacy of ICBs in melanoma.
Physiological provocation compared to acetazolamide in the assessment of cerebral hemodynamics – A case report
Authors: Henriksen Alexander Cuculiza , Thomsen Gerda Krog , Knudsen Gitte M , Stavngaard Trine , Rosenbaum Sverre , Marner Lisbeth 
Published: September 2024

Abstract

Background: Severe large vessel disease may lead to cerebral hemodynamic failure that critically impairs cerebral blood flow (CBF) regulation elevating the risk of ischemic events. Assessment of the condition is often based on changes in CBF during vasodilatation; however, pharmacologically induced vasodilation does not reflect the physiological condition during an ischemic event caused by hemodynamic failure. We compared a [15O]H2O PET brain scan during vasodilation to a [99mTc]HMPAO SPECT brain scan during an ongoing transient ischemic attack (TIA).
Case presentation: A single patient presenting with limb-shaking TIA underwent CT, Digital Subtraction Angiography, and two cerebral perfusion scans: [15O]H2O PET and [99mTc]HMPAO SPECT. Acetazolamide was used in the PET scan to induce vasodilatation, and during the SPECT scan physiological stress, standing up rapidly, was used to induce limb-shaking TIA.
CT-angiography and Digital Subtraction Angiography demonstrated an occluded anterior cerebral artery distal to the circle of Willis on the left side, with a corresponding infarction in the watershed area. Collaterals supplied the main vascular territory of the anterior cerebral artery. During rest, neither perfusion scans demonstrated reduced perfusion outside of the ischemic core. However, we found a pronounced difference between the PET utilizing acetazolamide and the SPECT during the TIA. The PET scan demonstrated a reduced CBF in vascular territory supplied by collaterals, while the area around the ischemic core was not affected. Contrary, the SPECT had only a minor decrease in the collateral-supplied area, whereas the watershed area proximal to the infarct core had a pronounced decrease.
Conclusions: The observed discrepancy in compromised areas during physiological provocation compared to pharmacological induced vasodilation questions the use of an unphysiological stressor for assessment of cerebrovascular hemodynamics. A physiological provocation test may achieve more clinically relevant evaluation.
Exploring the Causal Effect of Dietary Factors on Hemorrhoids: A Two-Sample Mendelian Randomization Study
Authors: Hu Zongju , Zhao Zongxian , Yao Yuan , Su Xinyu , Zhu Shu , Zhang Jun 
Published: September 2024

Abstract

Background
Previous research has found that dietary factors are closely associated with the risk of hemorrhoids. Modifying dietary structure and offering evidence-based dietary guidance are crucial for preventing and managing hemorrhoids disease. Our study aims to investigate the causal effects of 17 dietary factors on the risk of hemorrhoids using a two-sample mendelian randomization (MR) approach.
Method
Genetic variants and instrumental variables for dietary factors (exposure datasets) were obtained from UK Biobank (UKB), and hemorrhoids (outcome datasets) related single-nucleotide polymorphisms (SNPs) were extracted from the European Bioinformatics Institute (EBI). Eligible SNPs were selected for subsequent MR analysis and the Inverse Variance Weighted (IVW) method served as the primary analytical approach. Finally, we conducted sensitivity analyses and heterogeneity tests on the MR results
Result
Our findings indicated that vegetables (OR: 0.591, 95% CI: 0.428–0.817, p = 0.010) and fruits (OR: 0.764, 95% CI: 0.639–0.915, p = 0.005) intake has a protective effect, while alcohol intake (OR: 1.588, 95% CI: 1.246–2.024, p < 0.001), processed meat (OR: 1.307, 95% CI: 1.077–1.585, p = 0.007), oily fish (OR: 1.119, 95% CI: 1.014–1.235, p = 0.025), and cereal (OR: 1.209, 95% CI: 1.036–1.411, p = 0.016) intake have been identified as risk factors for hemorrhoids. Indeed, the causal relationships between above dietary factors and hemorrhoids were robust under the MR pleiotropy residual sum and outlier (MR-PRESSO) test and heterogeneity test.
Conclusions
This study offers scientific dietary guidance for patients and aims to decrease the incidence of hemorrhoids by elucidating the specific effects of dietary factors.
High Diagnostic Performance of 68Ga-FAPI-based Techniques in Female Cancer Detection: Systematic Review and Meta-Analysis
Authors: Gabdullina Madina , Ainakulova Akmaral , Elboga Umut , Karibayeva Indira , Alisherov Galymzhan , Zhakenova Zhanar , Amankulov Jandos , Zholdybay Zhamilya 
Published: September 2024

Abstract

Positron emission tomography (PET) imaging techniques combined with computed tomography (PET/CT) or magnetic-resonance imaging (PET/MRI) have been developed in the last decades to improve primary diagnosis, staging, restaging, planning radiotherapy, and assessment of therapy efficacy for cancer patients. Currently, the most widely recognized and well-studied radiopharmaceutical is 2-deoxy-2[18F]fluoro-D-glucose ([18F]-FDG). However, [18F]-FDG has some limitations related to physiological accumulation in various organs and low avidity for some histological subtypes. Interest in Gallium 68 ([68Ga])-labeled fibroblast activation protein inhibitor (FAPI), which may have high sensitivity and specificity, has increased following the identification of FAP. We reviewed the current literature on [68Ga]-FAPI-based PET imaging techniques in the detection of female cancers and conducted a meta-analysis to determine the diagnostic performances of [68Ga]-FAPI-based PET imaging techniques for breast and gynecological cancers.
Improved gastrointestinal tolerance and iron status via probiotic use in iron deficiency anemia patients initiating oral iron replacement
Authors: şahintürk yasin , köker gökhan , köker gülhan özçelik , coşkuner ali , durmuş merve eren , çatlı mehmet mutlu , çekin ayhan hilmi 
Published: September 2024

Abstract

Background
Strategies that consider enhancing iron absorption and reducing gastrointestinal burden are important for effective iron replacement therapy (IRT) in patients with iron deficiency anemia (IDA)
Aim
To investigate gastrointestinal tolerability, treatment compliance and iron status markers in patients with IDA who received oral IRT with versus without concomitant Lactobacillus plantarum 299v (L. plantarum 299v) probiotic supplementation
Methods
A total of 295 patients with newly diagnosed IDA who were randomly assigned to receive either IRT alone (n = 157, IRT-only group) or IRT plus L. plantarum 299v (n = 138, IRT-Pro group) were included in this prospective randomized non-placebo, controlled study. Gastrointestinal intolerance symptoms, serum hemoglobin levels and iron status markers were recorded in each group.
Results
Overall, 91 (30.8%) of 295 patients reported gastrointestinal intolerance symptoms during IRT, and 30(10.2%) discontinued IRT within the first 30 days of treatment. Patients in the IRT-Pro group compared to those in the IRT-only group experienced significantly lower rates of gastrointestinal intolerance over the course of IRT (13.0% vs. 46.5%, p < 0.001) and treatment discontinuation within the first 30 days (3.6% vs. 15.9%, p < 0.001). At 3rd month of therapy, IRT-Pro vs. IRT-only group had significantly higher serum levels for iron (76.0(51.0–96.0) vs. 60.0(43.0–70.0) µg/dL, p < 0.001) and transferrin saturation (20.1(12.5–28.5) vs. 14.5(10.5–19.0) %, p < 0.001), in addition to higher change from baseline hemoglobin (0.9(0.3–1.3) vs. 0.4(-0.1-1.1) g/dL, p < 0.001) levels.
Conclusion
Use of L. plantarum 299v probiotic supplementation during the first 30 days of IRT in IDA patients significantly reduces the gastrointestinal burden of IRT (particularly abdominal pain and bloating), the likelihood of intolerance development (by ~ 3 times) and treatment discontinuation (by ~ 5 times), as accompanied with improved serum hemoglobin levels and serum iron markers.
Single Cell Droplet-Based Efficacy and Transcriptomic Analysis of a Novel Anti-KLRG1 Antibody for Elimination of Autoreactive T Cells
Authors: Konry Tania , Sulllivan Matthew , Rozzo Aldo , Ward Antonio , Rao Patricia , Soler-Ferran Dulce , Greenberg Steven 
Published: September 2024

Abstract

Progress in developing improvements in the treatment of autoimmune disease has been gradual, due to challenges presented by the nature of these conditions. Namely, the need to suppress a patient’s immune response while maintaining the essential activity of the immune system in controlling disease. Targeted treatments to eliminate the autoreactive immune cells driving disease symptoms present a promising new option for major improvements in treatment efficacy and side effect management. Monoclonal antibody therapies can be applied to target autoreactive immune cells if the cells possess unique surface marker expression patterns. Killer cell lectin like receptor G1 (KLRG1) expression on autoreactive T cells presents an optimal target for this type of cell depleting antibody therapy. In this study, we apply a variety of in vitro screening methods to determine the efficacy of a novel anti-KLRG1 antibody at mediating specific natural killer (NK) cell mediated antibody-dependent cellular cytotoxicity (ADCC). The methods include single-cell droplet microfluidic techniques, allowing timelapse imaging and sorting based on cellular interactions. Included in this study is the development of a novel method of sorting cells using a droplet-sorting platform and a fluorescent calcium dye to separate cells based on CD16 recognition of cell-bound antibody. We applied this novel sorting method to visualize transcriptomic variation between NK cells that are or are not activated by binding the anti-KLRG1 antibody using RNA sequencing. The data in this study reveals a reliable and target-specific cytotoxicity of the cell depleting anti-KLRG1 antibody, and supports our droplet-sorting calcium assay as a novel method of sorting cells based on receptor activation.