bluuebunny/crossref_metadata_2025_split huggingface dataset:
The evolution and developmental dynamics of histone-based chromatin regulation in Annelida
Authors: Martín-Zamora Francisco M. , Cole Joby , Donnellan Rory D. , Guynes Kero , Carrillo-Baltodano Allan M. , Dickman Mark J. , Hurd Paul J. , Martín-Durán José M. 
Published: September 2024

AbstractEukaryotic histones protect and package nuclear DNA into nucleosomes. The dynamic addition and removal of posttranslational modifications on these proteins define regulatory regions that play a central role in genome and chromatin biology. However, our understanding of these regulatory mechanisms in animals is largely based on a few model systems, which prevents a general understanding of how histone-based regulation unfolds and promotes phenotypic variation during animal embryogenesis. Here, we apply a comprehensive multi-omics approach to dissect the histone-based regulatory complement in Annelida, one of the largest invertebrate phyla. Annelids exhibit a conserved histone repertoire organised in clusters of dynamically regulated, hyperaccessible chromatin. However, unlike other animals with reduced genomes, the wormDimorphilus gyrociliatusshows a dramatically streamlined histone repertoire, revealing that genome compaction has lineage-specific effects on histone-based regulation. Notably, the annelidOwenia fusiformishas two H2A.X variants that co-occur in other animals, whose functional implications are unclear but represent a unique case of widespread parallel evolution of a histone variant in Eukarya. Histone-modifying enzyme complements are largely conserved amongst annelids. Yet, temporal differences in the expression of a reduced set of histone modifiers correlate with distinct ontogenetic traits and variation in the adult landscapes of histone modifications, as revealed by quantitative mass spectrometry inO. fusiformisandCapitella teleta. Collectively, our unparalleled analysis of histone-based epigenetics within a non-model phylum informs the evolution of histone-based regulation, presenting a framework to explore how this fundamental genome regulatory layer contributes to developmental and morphological diversification in annelids and animals generally.
Common regulatory mutation increases single-cell survival to antibiotic exposures inPseudomonas aeruginosa
Authors: Ritz David , Deng Yijie , Schultz Daniel 
Published: September 2024

AbstractTypical antibiotic susceptibility testing (AST) of microbial samples is performed in homogeneous cultures in steady environments, which does not account for the highly heterogeneous and dynamic nature of antibiotic responses. The most common mutation found inP. aeruginosalineages evolved in the human lung, a loss of function of repressor MexZ, increases basal levels of multidrug efflux MexXY, but does not increase resistance by traditional MIC measures. Here, we use single cell microfluidics to show thatP. aeruginosaresponse to aminoglycosides is highly heterogeneous, with only a subpopulation of cells surviving exposure.mexZmutations then bypass the lengthy process of MexXY activation, increasing survival to sudden drug exposures and conferring a fitness advantage in fluctuating environments. We propose a simple “Response Dynamics” assay to quantify the speed of population-level recovery to drug exposures. This assay can be used alongside MIC for resistance profiling to better predict clinical outcomes.
Thalamic burst and tonic firing selectively indicate patients’ consciousness level and recovery
Authors: Wang Huan , Hu Yongxiang , Ge Qianqian , Dang Yuanyuan , Yang Yi , Xu Long , Xia Xiaoyu , Zhang Peng , He Sheng , Laureys Steven , Yang Yan , He Jianghong 
Published: September 2024

AbstractPatients with disorders of consciousness exhibit severe declines in arousal and awareness, alongside anomalous functional brain connections and aberrant neuronal activities1–4. Yet, the diagnostic error of patients’ consciousness states can reach up to forty percent5,6, resulting in a worse prognosis. Neuronal mechanisms underlying the disorders are indispensable for identifying objective and intrinsic markers of consciousness. As the principal relay station between the brainstem arousal nuclei and the cerebral cortex, the thalamus has been empirically inferred to maintain consciousness and wakefulness within the brain connectome7–12. Here, we investigated thalamic spiking, brain connections, consciousness states, and outcomes following deep brain stimulation in 29 patients. Our study reveals that thalamic activities can signal their consciousness states. Patients diagnosed with vegetative state/unresponsive wakefulness syndrome exhibited less active neurons with longer and more variable burst discharges compared to those in a minimally conscious state. Furthermore, as a direct deep brain stimulation site, neuronal profiles in the centromedian/parafascicular complex of the thalamus indicated whether electrostimulation here improved outcomes. Stronger tonic firing was associated with enhanced thalamocortical connections and better recovery outcomes in patients. These findings suggest that thalamic spiking signatures, including single-neuron burst discharge and tonic firing, selectively indicate the representation and alteration of consciousness. These findings provide direct neuronal and clinical evidence for understanding of thalamic contributions to disorders of consciousness.
Genome-wide Association Study of Social Isolation in 63 497 Japanese Individuals from the General Population
Authors: Ohseto Hisashi , Inoue Kosuke , Takahashi Ippei , Obara Taku , Narita Akira , Ishikuro Mami , Orui Masatsugu , Murakami Keiko , Noda Aoi , Shinoda Genki , Takase Masato , Nakaya Naoki , Kogure Mana , Hatanaka Rieko , Nakaya Kumi , Chiba Ippei , Tokioka Sayuri , Kotozaki Yuka , Shimizu Atsushi , Tanno Kozo , Hozawa Atsushi , Tamiya Gen , Kondo Naoki , Kuriyama Shinichi 
Published: September 2024

AbstractSocial isolation, characterized by a lack of social connections with family, friends, and others, is associated with adverse health outcomes. However, the genetic contribution to the susceptibility to social isolation remains unclear. This study aimed to identify genetic loci associated with social isolation using the Lubben Social Network Scale (LSNS-6) in a Japanese population. The Tohoku Medical Megabank Community-Based Cohort Study was conducted between 2013 and 2016. The participants were genotyped using the Affymetrix Axiom Japonica Array. The LSNS-6 was used to assess familial and friend ties through six questions and social isolation statuses were defined as total scale, family subscale, and friend subscale. Genome-wide association studies (GWASs) were conducted using a generalized linear mixed model, adjusting for age, sex, and 10 genetic principal components. In total, 63 497 participants who completed genotyping and the LSNS-6 were included. The mean age was 59.4±11.9 years, and 41 126 (64.8%) were female. Significant genetic loci were identified in GWASs for the total scale (rs10736933 near ACADSB and HMX3) and friend subscale of LSNS-6 (rs1778366 near LINC02315 and LRFN5). This study provides the first genome-wide evidence of social isolation in the Japanese population, suggesting associations with ACADSB, HMX3, LINC02315, and LRFN5. These findings could enable personalized prevention and intervention for social isolation and related psychiatric disorders.
Bibliometric analysis of disaster nursing based on CiteSpace
Authors: Xie Haixia , Zhang Zhi , Han Ruijuan , Li Fulan , Yu Tianshuang 
Published: September 2024

AbstractBackgroundDisaster nursing as a response to natural disasters, accidents disasters and other emergencies important areas, on a global scale in recent years received wide attention and research. This study discusses the research status and development trend of disaster nursing in the past 10 years, to provide a reference for future research and practice.MethodsLiterature in disaster nursing from 2013 to 2023 was searched using the Web of Science database, and CiteSpace 6.1.R6 software was used for visual analysis.ResultsA total of 4865 articles were retrieved, identified, and presented with a trend of steady rise. The United States has the largest number and has significant influence in the field. Disaster preparedness, disaster resilience, and disaster nursing education are the research hotspots. The combination of disaster nursing and intelligent technology will be the future research trend.ConclusionIn the last 10 years, research on disaster nursing has shown a diversified and comprehensive development trend. In the future, with the continuous impact of global climate change and the increasing social demand for disaster nursing, the research and practice of disaster nursing will pay more attention to interdisciplinary cooperation, technological innovation, and humanistic care.
Applying item response theory to psychometrically evaluate and shorten the Negative Acts Questionnaire-Revised
Authors: Dåderman Anna M. , Kajonius Petri J. , Basinska Beata A. 
Published: September 2024

AbstractWorkplace bullying (WB) assessment often relies on the Negative Acts Questionnaire-Revised (NAQ-R). This study aimed to shorten and improve the NAQ-R using Item Response Theory (IRT) and address sex bias. IRT analysis from 867 Swedish employees (66% women) identified less-informative items. Based on this, a 13-item NAQ-R Short Form (NAQ-R-SF) was developed, demonstrating strong discrimination and validity. The new NAQ-R-SF showed a significant correlation with a primary WB measure (r = .57) and other relevant constructs, including individual factors like neuroticism and health quality, as well as work-related factors such as interpersonal conflicts and work performance. Sex bias was not found. IRT and validity evidence support the NAQ-R-SF as a robust tool for measuring WB, aligning with established WB constructs and individual differences.
Analysis of IL-4 Levels, IL-1α, Cortisol Hormone, and Blood Cell Number in Parkinson’s Disease Patients Undergoing Dance-based Movement Therapy: A Preliminary Study
Authors: Marques Karen Adriana Pastana , Bahia Gabriel Mesquita da Conceição , Duarte Juliana dos Santos , Pinto Denise da Silva , Martins Filho Arnaldo Jorge , de Jesus Oliveira Batista Evander , Oliveira da Silva Anderson Manoel Herculano , Matos Oliveira Karen Renata Herculano , Krejcova Lane Viana , Bahia Carlomagno Pacheco 
Published: September 2024

AbstractIntroductionThe interplay between neuroinflammation and Parkinson’s disease (PD) is still unclear, although several studies suggest that neuroinflammation plays an important role in the pathophysiological pathways of neurodegenerative disorders. Dance-based movement therapy has been studied as an adjuvant treatment for PD symptoms by modulating some inflammatory interleukins and improving the overall effect of pharmacological treatments.ObjectiveTo evaluate the levels of Interleukin 4, Interleukin 1α, cortisol hormone, and blood cell total numbers in PD subjects undergoing dance-based movement therapy.MethodsTwenty-five participants were selected for this study. Seventeen subjects diagnosed with PD were divided into two groups: PD group (n=8) and PD+dance group (n=9). Another group was composed of age-matched healthy controls (n=8). The dance-based movement therapy was performed twice a week for 50-60 minutes/session over six months.ResultsThe serum IL-4 levels were significantly lower in the PD group when compared to the healthy group, while the PD+Dance group showed similar levels to the healthy group. The levels of IL-1α, cortisol, and blood cell count showed no statistical differences between groups.ConclusionOur results suggest that regular practice of dance-based movement therapy may induce a neuroprotective effect by modulating the levels of IL-4 in people with PD similarly to healthy participants.Trial registrationThe study was registered in the Brazilian registry of clinical trials: RBR-3bhbrb5.
Learning geometric models for developmental dynamics
Authors: Howe Addison , Mani Madhav 
Published: September 2024

AbstractSuccessful development from a single cell to a complex, multicellular organism requires that cells differentiate in a coordinated and organized manner in response to a number of chemical morphogens. While the molecular underpinnings may be complex, the resulting phenomenon, in which a cell decides between one fate or another, is relatively simple. A body of work—rooted in dynamical systems theory—has formalized this notion of cellular decision making as flow in a Waddington-like landscape, in which cells evolve according to gradient-like dynamics within a potential that changes shape in response to a number of signals. We present a framework leveraging neural networks as universal function approximators to infer such a parameterized landscape from gene expression data. Inspired by the success of physics-informed machine learning in data-limited contexts, we enforce principled constraints motivated not by physical laws, but by this phenomenological understanding of differentiation. Our data-driven approach infers a governing landscape atop a manifold situated within expression space, thereby describing the dynamics of interest in a biologically meaningful context. The resulting system provides an intuitive, visualizable, and interpretable model of cellular differentiation dynamics.
The response of multi-disease and insect-resistant tomato lines to the accumulation of TYLCV, a whitefly-transmitted virus
Authors: Prabhakar Shruthi Shimoga , Hsu Yun-che , Yen Joyce , Chou Hsiu-yi , Lin Mei-ying , Priya M. Shanthi , Othim Stephen , Ramasamy Srinivasan , Eybishitz Assaf 
Published: September 2024

AbstractTomato (Solanum lycopersicum) is a major vegetable crop grown worldwide for its culinary versatility and nutritional richness. The whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) poses a significant threat to its cultivation. The management strategies for this disease include controlling the virus and/or the vector. The multi-disease and insect-resistant lines developed by the World Vegetable Centre (WorldVeg) contain Ty-1/Ty-3 genes for virus resistance and WF2-10 and WF3-09 genes for whitefly resistance. This study evaluates the efficacy of suppressing virus accumulation in multi-disease and insect-resistant tomato lines compared to Ty-resistant or whitefly-resistant lines and a susceptible check. Preference bioassays, controlled inoculation with viruliferous whiteflies, and acylsugar quantification revealed that multi-disease and insect-resistant lines, developed at WorldVeg, had significantly higher acylsugar concentration and were less preferred by the whitefly adults for settling and whiteflies had high adult mortality. The multi-disease and insect-resistant lines also showed less severe disease symptoms and reduced virus accumulation over time when compared to Ty-resistant, whitefly-resistant, and susceptible check. The findings reveal that the multi-disease and insect-resistant lines are superior in mitigating the threat posed by TYLCV compared to TYLCV-resistant lines. These results underscore the potential of combined virus and vector resistance in tomatoes as a key element of Integrated Pest Management strategies against whitefly-transmitted TYLCV, offering a sustainable solution for safeguarding tomato production.
Visitor effect on the behavior of chimpanzees (pan troglodyte) at a primate rescue center
Authors: Alegre Maurer Sofia , Ross Miriam , Gil Dolz Jose 
Published: September 2024

1.AbstractThe objective of this study was to examine the impact of visitors on the behavior of chimpanzees at a sanctuary. We hypothesized there wouldn’t be an increase in abnormal, agnostic, or self-directed behaviors during visits, nor a decrease in affiliative behaviors when visitors are present.The study examined the effects of visitor presence on chimpanzee behavior at Fundació MONA, a rescue center.Key findings includeNo significant changes in abnormal or self-directed behaviors or agonistic behaviors were observed with visitor presence.Affiliative behaviors (excluding grooming) showed a slight decrease during visits.These results indicate that guided visitor interactions do not adversely affect chimpanzee behavior and may even enhance their welfare. The study supports the implementation of structured visitor programs for public education and funding, without compromising animal well-being. This contrasts with some previous research in zoo settings, suggesting that controlled visits can be beneficial in primate sanctuaries.
Reusable Generic Clinical Decision Support System Module for Immunization Recommendations in Resource-Constraint Settings
Authors: Orlioglu Samuil , Boobalan Akash Shanmugan , Abanyie Kojo , Boyce Richard D. , Min Hua , Gong Yang , Sittig Dean F. , Biondich Paul , Wright Adam , Nøhr Christian , Law Timothy , Robinson David , Faxvaag Arild , Hubig Nina , Gimbel Ronald , Rennert Lior , Jing Xia 
Published: September 2024

AbstractClinical decision support systems (CDSS) are routinely employed in clinical settings to improve quality of care, ensure patient safety, and deliver consistent medical care. However, rule-based CDSS, currently available, do not feature reusable rules. In this study, we present CDSS with reusable rules. Our solution includes a common CDSS module, electronic medical record (EMR) specific adapters, CDSS rules written in the clinical quality language (CQL) (derived from CDC immunization recommendations), and patient records in fast healthcare interoperability resources (FHIR) format. The proposed CDSS is entirely browser-based and reachable within the user’s EMR interface at the client-side. This helps to avoid the transmission of patient data and privacy breaches. Additionally, we propose to provide means of managing and maintaining CDSS rules to allow the end users to modify them independently. Successful implementation and deployment were achieved in OpenMRS and OpenEMR during initial testing.
Effect of different lining paper materials and infusions on oviposition preference ofAedes aegypti(Diptera: Culicidae) gravid mosquitoes
Authors: Momen Mahfuza , Seheli Kajla , Hossain Md. Aftab , Ghosh Ananna , Hossain Md. Forhad 
Published: September 2024

AbstractAedes aegypti(Linnaeus) mosquito is a vector, responsible for increasing global public health concerns due to its rapid geographical spread and increasing vectorial capacity. Understanding the breeding behavior ofAe. aegyptiis crucial to control this vector and thereby limiting the spread of diseases. To study this mosquito breeding in the laboratories and ovitraps, different types of lining-papers, surrounding inner surface of a container along with different types of added infusions, are used. Lining-papers serve as surfaces for oviposition and infusions provide the essential environment for nurturing. The types of oviposition surfaces and infusions used in laboratory rearing facilities or ovitraps influence oviposition preference, changing the breeding behavior ofAe. aegyptigravid mosquitoes. This study presents a comparative study on oviposition preferences for six different types of lining-papers and six different types of infusions in a bioassay cage. ANOVA analysis shows a significant effect on oviposition preference of different types of lining-papers that served as oviposition surfaces. The highest oviposition activity was observed for the‘agri seed germination paper-75’lining-paper when normal tap water infusion was used. Likewise, statistical analysis shows that when a‘plain printing offset paper 80 GSM’lining-paper was used, a highly statistically significant effect on oviposition preference is observed for different types of infusions used in the oviposition cups.
Selection on time to parasite transmission shapes the hostAnopheles gambiaetranscriptional response and suggests immune evasion
Authors: Silva Luís M. 
Published: September 2024

AbstractUnderstanding host-parasite interactions is of the utmost importance for the correct disease prediction, prevention and management. Hence, this study assessed the transcriptional response of the primary malaria vector,Anopheles gambiae, to infection with several lines of the prominent vector-control parasite, the microsporidianVavraia culicis. These parasitic lines have been selected for early or late transmission within this host. Previous studies extensively described them phenotypically, differing in their virulence, infection dynamics and host exploitation. Using RNA sequencing, gene expression profiles were analyzed in mosquitoes infected with early-selected, late-selected, unselected (reference)V. culicislines and uninfected controls. The results revealed distinct transcriptional changes associated with each parasite line. Early-selected parasites induced a broader immune response than late-selected ones. Differential expression of immune-related genes, includingToll-interacting proteinandProtein ERGIC-53, suggests enhanced immune evasion in late-selected parasites. Additionally, significant changes were observed in pathways related to Golgi membrane function and oxidative stress response, particularly in response to early-selected parasites. These findings highlight the evolutionary pressures shaping host-parasite coevolution and provide insights into how parasite transmission traits can influence mosquito immune response and regulation. This work offers a foundation for future studies on mosquito-microsporidia dynamics and potential applications for vector control strategies, particularlyPlasmodium.
Charged current weak production of
Δ(1232)
induced by electrons and positrons
Authors: Fatima A. , Athar M. Sajjad , Singh S. K. 
Published: September 2024

The charged current weak production of Δ(1232) from the free proton target induced by the electron/positron in the intermediate energy range corresponding to the beam energy available at JLab and Mainz, has been studied. The results for the differential scattering cross section dσdQ2, the angular distribution dσdΩΔ, and the total scattering cross section σ(Ee) for both the electron and positron induced processes are presented, for the various energies in the range of 0.5–4 GeV. The cross section σ(Ee) is found to be of the order of 10−39  cm2 for the electron/positron energies in the few GeV range. The availability of electron/positron beams having well-defined energy and direction with very high luminosity of the order of 1038–1039  cm−2  sec−1, makes it possible to observe the weak charged current production of Δ(1232) and determine the axial vector form factors CiA(Q2);(i=3–5). The sensitivity of the differential cross section dσdQ2 to the subdominant form factors C3A(Q2) and C4A(Q2) is found to be strong enough, especially in the low Q2 region, which can be used to determine them phenomenologically and to test the various theoretical models proposed to calculate them.

Published by the American Physical Society
2024