bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unlocking the secrets of NPSLE: the role of dendritic cell-secreted CCL2 in blood-brain barrier disruption
Authors: Wang Lei , Zheng Guimin , Wang Peiwen , Jia Xiuchuan 
Published: September 2024

BackgroundThis study employed RNA-seq technology and meta-analysis to unveil the molecular mechanisms of neuropsychiatric systemic lupus erythematosus (NPSLE) within the central nervous system.MethodsDownloaded transcriptomic data on systemic lupus erythematosus (SLE) from the Gene Expression Omnibus (GEO) and analyzed differential genes in peripheral blood samples of NPSLE patients and healthy individuals. Employed WGCNA to identify key genes related to cognitive impairment and validated findings via RNA-seq. Conducted GO, KEGG, and GSEA analyses, and integrated PPI networks to explore gene regulatory mechanisms. Assessed gene impacts on dendritic cells and blood-brain barrier using RT-qPCR, ELISA, and in vitro models.ResultsPublic databases and RNA-seq data have revealed a significant upregulation of CCL2 (C-C motif chemokine ligand 2) in the peripheral blood of both SLE and NPSLE patients, primarily secreted by mature dendritic cells. Furthermore, the secretion of CCL2 by mature dendritic cells may act through the RSAD2-ISG15 axis and is associated with the activation of the NLRs (Nod Like Receptor Signaling Pathway) signaling pathway in vascular endothelial cells. Subsequent in vitro cell experiments confirmed the high expression of CCL2 in peripheral blood dendritic cells of NPSLE patients, with its secretion being regulated by the RSAD2-ISG15 axis and inducing vascular endothelial cell pyroptosis through the activation of the NLRs signaling pathway. Clinical trial results ultimately confirmed that NPSLE patients exhibiting elevated CCL2 expression also experienced cognitive decline.ConclusionsThe secretion of CCL2 by dendritic cells induces pyroptosis in vascular endothelial cells, thereby promoting blood-brain barrier damage and triggering cognitive impairment in patients with systemic lupus erythematosus.
Linarine inhibits inflammatory responses in dry eye disease mice by modulating purinergic receptors
Authors: Liu Pei , Jiang Pengfei , Tan Kang , Yu Yunfeng , Qin Genyan , Liu Tingting , Tian Sainan , Peng Jun , Peng Qinghua 
Published: September 2024

BackgroundLinarine is a natural chemical component widely found in Buddleja officinalis Maxim., Chrysanthemum indicum L., Mentha canadensis L., and other medicinal plants. Modern pharmacological studies have shown that linarine with good anti-inflammatory and antioxidant activities can inhibit the proliferation and induce apoptosis of many kinds of tumor cells. Moreover, linarine showed protective effect on the liver, kidneys, and other organs.MethodsInflammation model of human corneal epithelial cell (HCEC) was constructed using NaCl induction, and cytotoxicity was detected by the CCK8 assay. The levels of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin 1β (IL-1β) were measured using Enzyme-linked immunoassay (ELISA). Chronic painful stimulation (tail clamping) in combination with Benzalkonium Chloride Solution drops in a desiccator established a mouse model of dry eye disease (DED). The following parameters were recorded: body mass, anal temperature, tear secretion, tear film rupture time, and corneal fluorescein staining. The levels of inflammatory factors mitogen activated protein kinase (MAPK), nuclear factor kappa-B (NF-kB), c-Jun N-terminal kinase (JNK), IL-1β, Interleukin 18(IL-18), A2A, A3, P2X4, P2X7, P2Y1 were measured by using immunofluorescence (IF) staining.ResultsLinarine can inhibit the secreation of TNF-α, and IL-1β in HCECs. Linarine prolonged tear film rupture time, promoted tear secretion, repaired corneal damage, and reduced the levels of inflammatory factors of MAPK, NF-kB, JNK, IL-1β, IL-18, and modulated the levels of the purinergic receptor.ConclusionsLinarine is effective in treating dry eye in mice by inhibiting purinergic receptors-mediated inflammatory response.
Development and validation of a prognostic score for TIPS placement in patients with viral hepatitis cirrhosis-related portal hypertension: a multi-center retrospective study
Authors: Que Zenglong , Wu Mingsong , Lai Shujie , Wang Lei , Mu Zhiyong , Yang Jinhui , Xiong Wei , Hu Hong , Liu Aimin , An Xuan , Yu Haodong , Cao Qin , Zhang Yanmei , Luo Wenwen , Wang Jun , Chen Dongfeng , Liu Fuquan , Zhang Dazhi , Wen Liangzhi 
Published: September 2024

IntroductionThere is no established scoring model focused on viral hepatitis patients to predict the prognosis after transjugular intrahepatic portosystemic shunt (TIPS). We aimed to develop and validate a novel model based on the largest cohort for better prediction of both short-term (1 year) and long-term (3 years) postoperative prognoses after TIPS in viral hepatitis cirrhosis-related portal hypertension patients.MethodsA total of 925 viral hepatitis cirrhosis-related portal hypertension patients who underwent TIPS from nine hospitals were divided into the training and external validation cohorts. A novel Viral-associated Index of Post-TIPS score (VIPs) model was developed after performing Cox regression analysis. The VIPs model was compared to five previous models, namely, Child–Pugh, MELD, ALBI, CCG, and FIPS. Furthermore, X-tile software was used to stratify patients into low-, medium-, and high-risk groups.ResultsThe VIPs model included age, ascites, albumin, prothrombin time, total bilirubin, and sodium for post-TIPS prognosis prediction. The model demonstrated satisfying predictive efficiency in both discrimination and calibration, with an area under the curve of 0.781/0.774 (1 year/3 years) in the training cohort and 0.771/0.775 (1 year/3 years) in the external validation cohort, respectively.DiscussionWe first developed and externally validated a novel VIPs model for better prediction of both short-term and long-term postoperative prognoses after TIPS in Chinese patients with viral hepatitis cirrhosis-related portal hypertension.
Machine learning-driven survival prediction in gestational trophoblastic neoplasms: a focus on PSTT and ETT prognosis
Authors: Alshwayyat Sakhr , Haddadin Zena , Alshwayyat Mustafa , Alshwayyat Tala Abdulsalam , Odat Ramez M. , Al-kurdi Mohammed Al-mahdi , Kharmoum Saoussane 
Published: September 2024

IntroductionThe clinicopathological characteristics and prognosis of placental site trophoblastic tumor (PSTT) and epithelioid trophoblastic tumor (ETT) have not been well summarized. Consequently, we conducted the largest to date series of samples of both types and employed machine learning (ML) to assess treatment effectiveness and develop accurate prognostic models for patients with GTN. Gestational choriocarcinoma (GCC) was used as the control group to show the clinical features of PTSS and ETT.MethodsThe Surveillance, Epidemiology, and End Results (SEER) database provided the data used for this study’s analysis. To identify the prognostic variables, we conducted Cox regression analysis and constructed prognostic models using five ML algorithms to predict the 5-year survival. A validation method incorporating the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was used to validate the accuracy and reliability of the ML models. We also investigated the role of multiple therapeutic options using the Kaplan-Meier survival analysis. ResultsThe study population comprised 725 patients. Among them, 139 patients had ETT, 107 had PSTT, and 479 had GCC. There were no significant differences in survival between the different tumor groups. Multivariate Cox regression analysis revealed that metastasis was a significant prognostic factor for GCC, while older age and radiotherapy were significant prognostic factors for PTSS and ETT. ML models revealed that the Gradient Boosting classifier accurately predicted the outcomes, followed by the random forest classifier, K-Nearest Neighbors, Logistic Regression, and multilayer perceptron models. The most significant contributing factors were tumor size, year of diagnosis, age, and race.DiscussionOur study provides a method for treatment and prognostic assessment of patients with GTN. The ML we developed can be used as a convenient individualized tool to facilitate clinical decision making.
Influence of problem-solving ability and personality variables on the improvement and creativity of tactical decisions in basketball
Authors: Díaz-Rodríguez Carlos , Pérez-Córdoba Eugenio 
Published: September 2024

BackgroundBasketball players are increasingly required not only to read the tactical actions that take place in a game but also to make correct tactical decisions. This includes greater creativity in the type of solutions they must provide when faced with various tactical situations. To acquire these skills, young players need training in which personality and general intelligence variables significantly influence the learning process.MethodsA Perceptual Tactical Training (PTT) program was implemented, correlated with the Eysenck Personality Questionnaire and the Raven’s Progressive Matrices test, to observe improvements in tactical perception and decision-making among young basketball players.ResultsThe PTT produced significant improvements in tactical solutions and tactical perception for all players. Players who scored high in general intelligence found it easier to perceive tactical situations but struggled to generate original tactical solutions. Conversely, players with high psychoticism and high general intelligence were more capable of creating innovative tactical solutions but did not excel in perceiving tactical game situations.ConclusionTherefore, it is crucial to be aware of the individual differences in intelligence and personality traits among young players to enhance their tactical decision-making and creative abilities.
Understanding resilience among migrant women in a humanitarian reception center: a qualitative study
Authors: Jiménez-Lasserrotte María del Mar , Sánchez-Ojeda María Angustias , Vázquez-González Gloria , Ruiz-Fernández María Dolores , Peña-Rodríguez Azahara , Fernández-Medina Isabel María , Granero-Molina José 
Published: September 2024

BackgroundThe European Union receives thousands of irregular migrants and refugees annually. Irregular migrant women are admitted to Humanitarian Reception Centers. These migrants face multiple adversities on their migration journey, and resilience is key to coping with process. The aim of this study was to describe and understand irregular migrant women’s experience of resilience when living in humanitarian reception centers.MethodsDescriptive qualitative study. In-depth interviews and a focus group were carried out with 21 migrant women from different African countries, with an average age of 31.8 years. Thematic analysis was used to analyze the qualitative data using ATLAS.ti computer software.ResultsThree main themes emerged: (1) Irregular migrant women in transit: extreme vulnerability. (2) Migration support networks. (3) Promoting irregular migrant women’s resilience.ConclusionThe harshness of the migration process tests the resilience of irregular migrant women, who are a vulnerable group at high risk of social exclusion. Their time in humanitarian reception centers is significant in their process of developing resilient behaviors. The multidisciplinary resources of the humanitarian reception center help the irregular migrant women in their personal development.
Electronic cigarette use and consumption patterns in medical university students
Authors: Dilektasli Asli Gorek , Guclu Ozge Aydin , Ozpehlivan Arzu , Durak Vahide Aslihan , Gezmis Izzet , Ozgur Anıl , Cinar Burak , Demirdogen Ezgi , Ozturk Nilufer Aylin Acet , Ozkaya Guven , Coskun Funda , Ursavas Ahmet , Uzaslan Esra , Karadag Mehmet 
Published: September 2024

BackgroundA major public health hazard is youth e-cigarette use. Although new, e-cigarette health hazards are becoming well-known in the literature. E-cigarette sale restrictions and laws differ globally. In this cross-sectional study, we studied medical university students’ tobacco and e-cigarette use and characteristics in a country where sales and import of e-cigarettes are banned. The primary objective is to determine the prevalence of electronic cigarette use and understand consumption patterns among medical faculty students in this setting.Materials and methodsThe questionnaire was sent using a web-based student information system. Sociodemographic features, tobacco and e-cigarette use, consumption patterns, and e-cigarette risk perceptions were covered in 54 questions.ResultsThe study comprised 1,054 students (48.7% male) aged 21.5 ± 2.6 years who completed the questionnaire. 37.7%, 20.9% and 23.6% have smoked cigarettes, e-cigarettes, or water pipes. Current cigarette smokers were 17.0%, e-cigarette users 4.0%, and water pipe smokers 4.5%. E-cigarette users were 52.3% dual smokers. The most common symptoms reported by e-cigarette users were cough (58.4%) and dyspnea (54.2%). Multivariable models showed that the male sex, greater monthly income, and a current smoker friend were independent risk factors for e-cigarette ever use, while the male sex, paternal current smoking, and close friends’ current smoking status were risk factors for dual use among medical trainees. Many medical students who used electronic cigarettes underestimated nicotine’s health hazards and harmful chemicals in e-cigarettes. Despite e-cigarette sales being prohibited in our country, 56.4% and 25.4% of e-cigarette users provided e-cigarettes from tobacco shops and through online sales, respectively.ConclusionMedical university students use tobacco most often by smoking cigarettes. Despite medical university students being aware of the health hazards of e-cigarettes, the current use of electronic cigarettes is 4.0%. Male sex, greater monthly income, and having current smoker friends are independent risk factors for e-cigarette use, while paternal smoking is a risk factor for dual use among medical trainees. Although in the country, sales of e-cigarettes are banned, ever-use rates for e-cigarettes were remarkably high at 20.9%, and the ease of accessing e-cigarettes was striking.
Progress made in digitalizing antimicrobial resistance surveillance in a One Health approach in Kenya
Authors: Chuchu Veronicah M. , Njung’e Joseph , Muasa Bridgit , Gathira Muchira , Olela Geoffrey , Bubi Kinuthia S. , Ashaba Justus , Githii Susan , Ndanyi Romona , Tanui Emmanuel , Irungu John Irungu , Azegele Allan , Kadam Rigveda , Ferreyra Cecilia 
Published: September 2024

BackgroundAntimicrobial resistance (AMR) is a major threat to global public health, affecting human and animal health, agriculture, food safety, and the environment. The control of AMR is often challenging, particularly when data are scanty or siloed in individual sectors. To develop evidence-based control policies for AMR, an electronic information system that integrates AMR data from various sectors, in a One Health approach, is critical.MethodologyAcknowledging the interconnectedness of AMR in humans, animals, and the environment and the need to assess the AMR burden using a One Health approach, Kenya’s National Antimicrobial Stewardship Interagency Committee (NASIC), with support from FIND, integrated human and animal health AMR data at the national AMR data repository and developed the One Health AMR Surveillance System (OHAMRS). The OHAMRS comprises two core digital components: interoperability middleware for integrating data from various sources and a DHIS2 web portal for the analysis and visualization of AMR surveillance data from the human and animal health sectors. These components are scalable for future inclusion of data from other One Health sectors, e.g., the environment, food/feed, and aquaculture sectors.ResultsThe OHAMRS has 42 dashboards that facilitate the presentation, interpretation, and dissemination of actionable information relating to AMR, including 17 dashboards for human and animal health priority pathogens and 8 for drug-resistance indicators. The priority pathogen dashboards provide visualization of antimicrobial susceptibility patterns, resistance and susceptibility trends, resistance tables, and geospatial susceptibility maps. Other dashboards include surveillance sites and specimen reports, data completeness, data reconciliation, sample testing workload, a One Health intersectoral dashboard, and other reporting tools for diverse stakeholders.DiscussionDigitalizing AMR surveillance through a One Health lens is pivotal to understand AMR prevalence and patterns across various sectors. The OHAMRS provides comprehensive data analysis and presentation, informing policymaking on AMR control. Digital tools such as the OHAMRS are vital in facilitating the availability of data and actionable information on AMR required to address the AMR crisis in Kenya.
Improved staffing policies and practices in healthcare based on a conceptual model
Authors: Wei Ning , Wang Zhongwei , Li Xiyuan , Zhang Yibing , Zhang Jing , Huang Ziying , Wang Xiao 
Published: September 2024

BackgroundStaffing policies are critical in healthcare facilities. However, problems from a mismatch between staff numbers requirements and offers are frequently encountered. This research examines the joint effects of quantitative and qualitative staff mismatch in a healthcare organization in China to understand how staffing management policies affect staffing adequacy and how staffing adequacy leads to important human resource (HR) outcomes.MethodsIn a previous study, we identified four dimensions of staffing management policies, namely decision-making, data management, productivity optimization, and title vs. competency. Based on this categorization, an 11-item scale was generated and evaluated for psychometric quality. A quantitative study was conducted with 1,323 healthcare professionals (including clinical and administrative staff) working at the hospital, matched by dyads and teams. A conceptual model with work engagement as a mediator between quantitative staffing adequacy interactions and qualitative staffing adequacy was tested with structural equations.ResultsThe findings indicate that staffing policies do exert effects on staffing adequacy. These findings further indicate that quantitative and qualitative staffing adequacy interact in explaining work engagement and team performance and that the moderated mediation occurs as hypothesized.ConclusionOur findings indicate that both types of staffing adequacies contribute to higher team performance via a heightened sense of work engagement from healthcare professionals. Furthermore, a modulation is observed between the two types of staffing adequacies during the promotion of higher team performance. The supported model is helpful in improving staffing management policies and increasing staffing fit so as to improve hospital performance.
Antibiotic susceptibility of Pseudomonas aeruginosa in Saudi Arabia: a national antimicrobial resistance surveillance study
Authors: Thabit Abrar K. , Alghamdi Ammar M. , Miaji Musaab Y. , Alharbi Feras S. , Jawah Anas F. , Alturki Fatimah , Hosin Nehal , Bazuqamah Mohammed , Almutairi Masaad Saeed , Alhamed Hamad , Elhendawy Alaa , Atallah Dalya , Humadi Abdulaziz A. , Alfifi Khalid A. , Alfadel Khadija , Eljaaly Khalid , Elfaky Mahmoud A. ,   the Saudi AntiMicrobial Surveillance (SAMS) study group
Published: September 2024

BackgroundPseudomonas aeruginosa is a common pathogen causing healthcare-associated infections. Most surveillance studies from Saudi Arabia that assessed the resistance by P. aeruginosa were conducted in single centers or did not use broth microdilution (BMD), the gold standard test. This is the first national multicenter study to assess the resistance profiles of P. aeruginosa isolates in Saudi Arabia using BMD.MethodsBetween 2022 and 2023, isolates from various infection sites were collected from seven hospitals in seven different regions of Saudi Arabia. The isolates were shipped to an academic microbiology lab, where their susceptibility was tested by BMD following Clinical Laboratory Standards Institute guidelines using Sensititre GNX3F plates. %Susceptibility to each antibiotic, and MIC50 and MIC90 were determined.ResultsIn total, 185 P. aeruginosa isolates were collected. Most isolates came from respiratory specimens (34.1%), followed by urine (21.1%) and skin/soft tissue (17.8%). The highest susceptibility was to amikacin (76.8%). Concurrently, susceptibility to meropenem was 52%, but it was 43.8% to colistin. While all P. aeruginosa isolates met the definition of multidrug-resistance, 41 (22.2%) were difficult-to-treat and 10 (5.4%) were pandrug-resistant. Difficult-to-treat isolates made up 30.3% of skin and soft tissue isolates, 25.4% of respiratory isolates, 21.7% of blood isolates, and 17.9% of urine isolates.ConclusionPseudomonas aeruginosa demonstrated an unexpectedly high level of resistance to several commonly used antibiotics. Therefore, antimicrobial stewardship and infection control policies should be strictly enforced by hospitals across the country to optimize treatment, prevent the emergence and spread of resistant strains, and track resistance trends with local antibiograms.
Modifying a multidisciplinary method to address challenging behavior in nursing home residents with dementia to involve family caregivers
Authors: Tasseron-Dries Petra E. M. , Smaling Hanneke J. A. , van der Steen Jenny T. , Achterberg Wilco P. 
Published: September 2024

IntroductionChallenging behavior and pain are common in nursing home residents with dementia. Challenging behavior and pain can be related and are stressful for residents, family caregivers and healthcare professionals. The STA OP! method provides a step-by-step protocol to manage challenging behavior and pain in nursing home residents with dementia. However, this method does not include a prominent and active role for family caregivers.MethodsThe STA OP! method was modified to include a role for family caregivers, in co-creation with family caregivers and healthcare professionals using elements of a realist approach. In separate meetings, two advisory groups comprised of family caregivers and professionals discussed ideas on how to involve family caregivers in STA OP!. Furthermore, barriers to involving family and possible solutions to overcome those barriers were discussed. Experts who had experience with the STA OP! method assessed the feasibility of the ideas in a nominal group technique meeting.ResultsThirty-eight ideas emerged in the advisory groups. The two ideas that generated the most discussion were Inviting family for a multidisciplinary meeting, and Assessment of pain in collaboration with family caregivers. Eventually, 21 ideas and suggestions to overcome possible barriers were included in a manual for the training of healthcare professionals in the adapted method, now called STA OP! with family.ConclusionHealthcare professionals and family caregivers collaborated well to shape the involvement of family caregivers in this method for managing challenging behavior and pain. The collected ideas supported by all involved resulted in a modified method: STA OP! with family and can now be tested in daily practice.