bluuebunny/crossref_metadata_2025_split huggingface dataset:
IFN-treated macrophage-derived exosomes prevents HBV-HCC migration and invasion via regulating miR-106b-3p/PCGF3/PI3K/AKT signaling axis
Authors: Chen Jing , Yin Qi , Xu Shiheng , Tan Xiaoqing , Liang Yu , Chen Chaohui , Li Li , Zhang Tao , Shen Tao 
Published: October 2024

BackgroundStudies revealed that exosomes from IFN-α-treated liver non-parenchymal cells (IFN-exo) mediate antiviral activity. MiR-106b-3p has been shown to play a paradoxical role in disease progressing from different studies. However, its specific role in HBV-related hepatocellular carcinoma (HBV-HCC) and the underlying mechanism remains unclear.MethodHuh7 cells transient transfected with plasmids of HBV-C2 and B3 were co-cultured with IFN-exo. Cell supernatants were collected to detect miR-106b-3p, HBsAg, HBeAg and HBV DNA levels. Cell proliferation, apoptosis, migration and invasion were analyzed. The putative targets of miR-106b-3p were identified by a dual-luciferase reporter system. The expression of PCGF3, migratory proteins(MMP2/9), and the PI3K/AKT signaling pathway-related proteins were assessed by western blot. The expression of PCGF3 mRNA was quantitative analyzed by using 52 pairs of paraffin-embedded tissues from HCC patients. siRNAs-PCGF3 were used to knocked-down PCGF3 expression.ResultsThe expression of miR-106b-3p was significantly higher in THP-1 cells and supernatants treated with IFN-exo than those untreated. Significantly increased expression of miR-106b-3p and decreased expression of HBsAg and HBV DNA were observed in Huh7-C2/B3 cells treated with IFN-exo. In addition, miR-106b-3p was directly target to PCGF3. Scratch healing assay and transwell assay showed that either IFN-exo or miRNA-106-3p over-expression, or siRNAs-PCGF3 inhibited migration and invasion of Huh7-C2/B3 cells, and subsequently resulted in suppression of p-AKT/AKT and p-PI3K/PI3K. Notably, the expression level of PCGF3 was significantly lower in HBeAg (+)-HCC tumor tissues than HBeAg (-)-HCC tumor.ConclusionIFN-α-induced macrophage-derived miR-106b-3p inhibits HBV replication, HBV- Huh7 cells migration and invasion via regulating PCGF3/PI3K/AKT signaling axis. miR-106b-3p and PCGF3 were potential biomarkers in the prevention and treatment of HBV-HCC.
Nitric oxide-producing monocyte-myeloid suppressor cells expand and accumulate in the spleen and mesenteric lymph nodes of Yersinia enterocolitica-infected mice
Authors: Leporati Marianela , Di Genaro María Silvia , Eliçabe Ricardo Javier 
Published: October 2024

IntroductionYersinia enterocolitica (Ye) is a Gram-negative bacterium that causes gastrointestinal infections. The myeloid-derived suppressor cells (MDSCs) constitute a cellular population with the capacity of inducing the specific suppression of T cells. Although there is evidence supporting the role of MDSCs in controlling the immune responses in several bacterial infections, its role during Ye infection has not yet been reported. Therefore, the purpose of the present work was to analyze MDSCs after oral Ye infection.MethodsC57BL/6 wild-type mice were infected with Ye WAP-314 serotype O:8. The proliferation of splenocytes and mesenteric lymph nodes (MLN) cells was measured as well as the levels of cytokines and nitric oxide (NO) in culture supernatants. The frequency and subsets of MDSCs were analyzed in the intestinal mucosa and spleen by flow cytometry. Furthermore, monocytic-MDSCs (Mo-MDSCs) and polymorphonuclear-MDSCs (PMN-MDSCs) were purified from the spleen of infected mice and their suppressor activity was evaluated in co-cultures with purified T cells.Resultswe observed a marked expansion of CD11b+Gr-1+ cells, a phenotype consistent with MDSCs, in the spleen and intestinal mucosa of Ye-infected mice. Interestingly, a robust proliferation of splenocytes and MLN cells was observed only when the MDSCs were depleted or the NO production was blocked. In addition, we determined that only Mo-MDSCs had the ability to suppress T-cell proliferation.ConclusionOur results highlight a mechanism by which Ye may induce suppression of the immune responses. We suggest that NO-producing Mo-MDSCs expand and accumulate in MLN and spleen of Ye-infected mice. These cells can then suppress the T-cell function without interfering with the anti-bacterial effector response. Instead, these immature myeloid cells may perform an important function in regulating the inflammatory response and protecting affected tissues.
Parents' color-conscious racial socialization and adolescents' racial attitudes: the moderating role of parental psychological control
Authors: Agalar Afra , Laible Deborah June , Carlo Gustavo , Liew Jeffrey 
Published: October 2024

IntroductionThe present two-wave longitudinal study examined the relation between White U.S. parents' color-conscious racial socialization for African Americans and adolescents' racial attitudes, and explored how parental psychological control might moderate this relation.MethodParticipants included 412 White U.S. adolescents (42% girls; Mage = 15.63 years, SD = 1.24) and their primary caregivers (52% mothers). They completed online questionnaires, with parents reporting on their color-conscious racial socialization at Time 1, and adolescents providing measures of their perception of parents' psychological control at Time 1, color-blind attitudes, and racial prejudice at both time points, ~16 months apart.ResultsOur findings revealed that parents' color-conscious racial socialization for African Americans was negatively associated with adolescents' negative racial attitudes. Additionally, parental psychological control moderated this relation: the negative association was evident only when parents exhibited low or average levels of psychological control.DiscussionThis study highlights the crucial role parents play in shaping their adolescents' racial attitudes and underscores the necessity of an autonomy-supportive environment to enhance the success of parents' color-conscious socialization practices. Understanding these dynamics is essential for developing effective interventions to reduce racial biases and foster inclusive attitudes among adolescents.
Spatial transcriptomics analysis identifies therapeutic targets in diffuse high-grade gliomas
Authors: Yang Yongtao , Hong Yingzhou , Zhao Kai , Huang Minhao , Li Wenhu , Zhang Kui , Zhao Ninghui 
Published: October 2024

IntroductionDiffuse high-grade gliomas are the most common malignant adult neuroepithelial tumors in humans and a leading cause of cancer-related death worldwide. The advancement of high throughput transcriptome sequencing technology enables rapid and comprehensive acquisition of transcriptome data from target cells or tissues. This technology aids researchers in understanding and identifying critical therapeutic targets for the prognosis and treatment of diffuse high-grade glioma.MethodsSpatial transcriptomics was conducted on two cases of isocitrate dehydrogenase (IDH) wild-type diffuse high-grade glioma (Glio-IDH-wt) and two cases of IDH-mutant diffuse high-grade glioma (Glio-IDH-mut). Gene set enrichment analysis and clustering analysis were employed to pinpoint differentially expressed genes (DEGs) involved in the progression of diffuse high-grade gliomas. The spatial distribution of DEGs in the spatially defined regions of human glioma tissues was overlaid in the t-distributed stochastic neighbor embedding (t-SNE) plots.ResultsWe identified a total of 10,693 DEGs, with 5,677 upregulated and 5,016 downregulated, in spatially defined regions of diffuse high-grade gliomas. Specifically, SPP1, IGFBP2, CALD1, and TMSB4X exhibited high expression in carcinoma regions of both Glio-IDH-wt and Glio-IDH-mut, and 3 upregulated DEGs (SMOC1, APOE, and HIPK2) and 4 upregulated DEGs (PPP1CB, UBA52, S100A6, and CTSB) were only identified in tumor regions of Glio-IDH-wt and Glio-IDH-mut, respectively. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) enrichment analyses revealed that upregulated DEGs were closely related to PI3K/Akt signaling pathway, virus infection, and cytokine-cytokine receptor interaction. Importantly, the expression of these DEGs was validated using GEPIA databases. Furthermore, the study identified spatial expression patterns of key regulatory genes, including those involved in protein post-translational modification and RNA binding protein-encoding genes, with spatially defined regions of diffuse high-grade glioma.DiscussionSpatial transcriptome analysis is one of the breakthroughs in the field of medical biotechnology as this can map the analytes such as RNA information in their physical location in tissue sections. Our findings illuminate previously unexplored spatial expression profiles of key biomarkers in diffuse high-grade glioma, offering novel insight for the development of therapeutic strategies in glioma.
Hydrolyzed protein formula improves the nutritional tolerance by increasing intestinal development and altering cecal microbiota in low-birth-weight piglets
Authors: Bai Miaomiao , Liu Hongnan , Yan Yalu , Duan Sufang , Szeto Ignatius Man-Yau , He Jian , Hu Jinjie , Fu Yawei , Xu Kang , Xiong Xia 
Published: October 2024

BackgroundPrematurity or low birth weight (LBW), poses a significant challenge in global health. Exploring appropriate and effective nutritional interventions is crucial for the growth and development of LBW infants. Hydrolyzed protein formula has been suggested as a potential solution to prevent intestinal dysfunction and improve digestion and absorption in infants.ObjectivesThis study aimed to investigate the benefits of hydrolyzed protein formula on feeding intolerance, intestinal morphological development, and microbiota in a LBW piglet model.MethodsA total of 24 male piglets (3 d of age, 0.95–1.25 kg average BW) were assigned (8 pens/diet; 1 pigs/pen) into three dietary treatments and fed with a basic formula (BF), standard premature infant formula (SF) and hydrolyzed protein formula (HF) respectively, for 7 d. After the piglets sacrifice, growth performance, amino acid metabolism and intestinal morphology were assessed. 16S rRNA sequencing and microbial metabolic phenotypes analyzed the effects of different formula treatments on intestinal flora structure of LBW piglets.ResultsThe HF diet reduced the rates of diarrhea and milk vomiting were reduced by 20.44% (p &gt; 0.05) and 58.44% (p &gt; 0.05), and decreased the crypt depth in the ileum while increasing the ratio of villus height/crypt depth and the mRNA expressions of y+LAT1 and b0,+AT in the ileum (p &lt; 0.05). HF increased the final body weight, serum Thr and essential amino acid contents, and CAT2 and b0,+AT mRNA expressions in ileal mucosa compared with the SF diet (p &lt; 0.05). Microbiota sequencing results showed that the colonic microbial richness indices (Chao1, ACE, and observed species), the diversity indices (Shannon and Simpson), and the phyla Actinobacteriota, unidentified_Bacteria, Acidobacteriota and Actinobacteria, the genus Rubrobacter and RB41 were reduced (p &lt; 0.05) in SF and HF groups. Microbial metabolic phenotypes analysis showed a reduction in the richness of biofilm-forming bacteria (p &lt; 0.05).ConclusionIn summary, hydrolyzed protein formula had better nutrition and tolerance in LBW suckling piglets by improving amino acid transport and intestinal development, and regulating gut microbial communities.
Association between household solid fuel usage and trajectories of multimorbidity among middle-aged and older adults: a nationwide population-based cohort study
Authors: Li Yiting , Wu Bingjie , Fan Bingbing , Lv Jiali , Li Chunxia , Su Chang , Liu Aidong , Zhang Tao 
Published: October 2024

BackgroundThis study aimed to explore the effect of household solid fuel usage on the multimorbidity trajectories among middle-aged and older adults.MethodsBased on the 2011–2018 China Health and Retirement Longitudinal Study, the group-based trajectory modeling and the multinomial logistic regression model were used to explore the relationship between multimorbidity trajectories of older adults with different fuel types, duration of solid fuel usage, and potential interaction with PM2.5. Three multimorbidity trajectory patterns were identified by group-based trajectory modeling and labeled as “non-chronic morbidity” (no disease increase), “newly developing multimorbidity” (diseases grew from 0 to 2), and “multi-chronic multimorbidity” (diseases grew from 2 to 4).ResultsCompared to “Non-chronic morbidity,” solid fuel was significantly associated with adverse multimorbidity trajectories, with an odds ratio (OR) and 95% confidence interval (CI) of 1.33 (1.11, 1.60) and 1.35 (1.18, 1.55) for newly developing and multi-chronic group, respectively. An adverse multimorbidity trajectory tended to be established with longer durations of solid fuel usage than “Non-chronic morbidity.” For “Newly-developing multimorbidity,” the ORs (95% CI) for 1–7 years and ≥ 8 years of solid fuel usage were 1.16 (0.94, 1.42) and 1.41 (1.12, 1.76), respectively, with P trend=0.001, while in “Multi-chronic multimorbidity,” those were 1.25 (1.07, 1.47) and 1.68 (1.41, 2.00), respectively, with P trend &lt;0.001. In the interaction analysis, the association between solid fuel usage and trajectories was significant only in areas where PM2.5 was lower than 50 μg/m3.ConclusionFor the middle-aged and older Chinese population, a higher risk of multimorbidity trajectory is associated with household solid fuel usage, especially in the areas with lower PM2.5.
Brief report: Aging adult utilization of an mHealth intervention for problem drinking
Authors: Sedotto Robyn N. M. , Edwards Alexandra E. , Dulin Patrick L. , King Diane K. 
Published: October 2024

Alcohol consumption among aging adults is a growing concern due to its potential to exacerbate age-related health conditions. Developing accessible interventions for this demographic is imperative. Mobile health (mHealth) interventions offer a promising avenue, but their effectiveness and engagement among aging adults remain uncertain. This study is a secondary analysis that aimed to compare the utilization and outcomes of an mHealth intervention between aging (50+) and younger adults in a clinical trial of an mHealth intervention (Step Away) for reduced drinking. At the three-month follow-up, both age groups exhibited significant reductions in alcohol consumption and increased readiness for change. Furthermore, aging adults utilized the mHealth intervention significantly more, expressed a higher likelihood of continued use, and rated the intervention higher on the System Usability Scale (SUS). These findings suggest that mHealth interventions for alcohol-related issues can be equally effective for aging adults and that they readily engage with such tools and find them acceptable. This study underscores the potential of mHealth interventions as a viable solution for addressing alcohol-related concerns among aging adults. Further research targeting mHealth interventions tailored specifically to this demographic is warranted.Clinical trial registration: https://clinicaltrials.gov/ct2/show/NCT04447794, Identifier [NCT04447794].
Determinants of Unmet Healthcare Needs During the Final Stage of the COVID-19 Pandemic: Insights From a 21-Country Online Survey
Authors: Lewis Samuel , Ewald Louisa , Duber Herbert C. , Mokdad Ali H. , Gakidou Emmanuela 
Published: October 2024

ObjectivesDuring the COVID-19 pandemic, essential health services experienced significant disruptions, impacting preventive and chronic care across the world.MethodsUtilizing the Pandemic Recovery Survey (PRS), conducted online with Facebook’s Active User Base across 21 countries between March and May 2023, this cross-sectional study identifies the magnitude of and key factors associated with unmet preventive and chronic care needs during the late stage of the COVID-19 pandemic.ResultsApproximately 28.2% of respondents reported unmet preventive care needs, and 42.1% experienced unmet chronic care needs, with key determinants including food insecurity (aOR 1.94, 95% CI 1.81–2.07 for preventive services; aOR 1.85, 95% CI 1.68–2.03 for existing conditions) and distrust in health professionals (aOR 1.09, 95% CI 1.03–1.15 for preventive services; aOR 1.53, 95% CI 1.41–1.66 for existing conditions).ConclusionThe findings underscore a widespread unmet need for health services, highlighting the impact of social determinants and trust in health professionals on service disruption. The results suggest that pandemic recovery efforts should focus on the most affected groups to bridge health disparities and ensure an equitable recovery.
Agave schidigera Transcriptome Reveals Stress-Responsive Phenylalanine ammonia-lyase Genes in Agave
Authors: Wang Xuxia , Hu Xiaoli , Lin Chen , Liu Qingqing , Li Yubo , Du Dengxiang , Mkapa Dietram , Zhang Weiyi , Huang Xing , Yi Kexian 
Published: October 2024

Agave is a significant fiber crop in tropical regions, known for its high fiber strength. Lignin is closely associated with fiber strength, and phenylalanine ammonia-lyase (PAL) serves as the initial enzyme in biosynthesis of lignin. Hence, it is of considerable significance to study the genes of PAL family to analyze the characteristics and mechanism of sisal fiber development. In this research, we conducted a transcriptomic analysis of Agave schidigera, a widely recognized ornamental plant in agave. Approximately 29.85 million clean reads were acquired through Illumina sequencing. In total, 116,602 transcripts including 72,160 unigenes were assembled, and 22.06~63.56% of those unigenes were annotated in public databases. Two, six, six and six PAL genes were successfully identified and cloned from A. schidigera, A. deserti, A. tequilana and A. H11648, respectively. After phylogenetic analysis, these genes were clustered into two branches. Genes AhPLA2a and AhPLA2c exhibited higher expression levels compared to other genes but had different expression patterns. Moreover, AhPLA2a and AhPLA2c were expressed at high levels under full-nutrient, nitrogen-free and phosphorus-free stresses. Most PAL genes were induced by Phytophthora nicotianae Breda, especially AhPAL1a, AhPAL1b, AhPAL2b and AhPAL2c. This research is the first work to present a de novo transcriptome dataset for A. schidigera, enriching its bioinformation of transcripts. The cloned PAL genes and the expression analyses will form the basis of future research on lignin biosynthesis, the relationship between lignin and fiber strength, and stress resistance in Agave species.
Physiological Effects and Mechanisms of Chlorella vulgaris as a Biostimulant on the Growth and Drought Tolerance of Arabidopsis thaliana
Authors: Moon Jinyoung , Park Yun Ji , Choi Yeong Bin , Truong To Quyen , Huynh Phuong Kim , Kim Yeon Bok , Kim Sang Min 
Published: October 2024

Microalgae have demonstrated biostimulant potential owing to their ability to produce various plant growth-promoting substances, such as amino acids, phytohormones, polysaccharides, and vitamins. Most previous studies have primarily focused on the effects of microalgal biostimulants on plant growth. While biomass extracts are commonly used as biostimulants, research on the use of culture supernatant, a byproduct of microalgal culture, is scarce. In this study, we aimed to evaluate the potential of Chlorella vulgaris culture as a biostimulant and assess its effects on the growth and drought tolerance of Arabidopsis thaliana, addressing the gap in current knowledge. Our results demonstrated that the Chlorella cell-free supernatant (CFS) significantly enhanced root growth and shoot development in both seedlings and mature Arabidopsis plants, suggesting the presence of specific growth-promoting compounds in CFS. Notably, CFS appeared to improve drought tolerance in Arabidopsis plants by increasing glucosinolate biosynthesis, inducing stomatal closure, and reducing water loss. Gene expression analysis revealed considerable changes in the expression of drought-responsive genes, such as IAA5, which is involved in auxin signaling, as well as glucosinolate biosynthetic genes, including WRKY63, MYB28, and MYB29. Overall, C. vulgaris culture-derived CFS could serve as a biostimulant alternative to chemical products, enhancing plant growth and drought tolerance.
Changes in heavy metal levels in the oral fluid of young patients with and without plaque-induced gingivitis undergoing treatment with braces
Authors: Kosyreva T. F. , Gorshunova N. V. , Katbeh I. , Abakeliya K. , Al Okbi M. A. , Lhamza G. 
Published: October 2024

Relevance. Research into the effects of nickel-titanium alloys and heavy metal ions on the development of inflammatory oral diseases is becoming increasingly significant in orthodontics. The relationship between toxic elements and inflammatory responses remains under-researched and controversial. Data on the connection between heavy metals and inflammatory reactions could influence the choice of orthodontic appliance materials and guide the subsequent management and monitoring of patients with braces. The question of the safe use of nickel-titanium alloy braces continues to be a relevant concern.Purpose. To assess the levels of heavy metals in the oral fluid of young patients with braces, both in those who developed plaque-induced gingivitis and those who did not.Materials and Methods. The study involved 50 clinically healthy patients (Groups I and II health categories) with intact teeth, including patients with compensated dental caries (isolated carious lesions – Grade I) and no periodontal diseases (PMA index <20), presenting with dental crowding and malocclusion (K07.2, K07.3). The average age of the patients was 22.1 ± 2.7 years, all of whom were undergoing treatment with metal braces. Oral fluid samples were analyzed for 12 heavy metal elements using inductively coupled plasma mass spectrometry (ICP-MS) in a laboratory setting. Additionally, lysozyme activity and oral fluid pH were measured before appliance placement and six months into orthodontic treatment.Results. Adaptation to braces in young patients is frequently complicated by the development of gingivitis and periodontitis due to impaired oral hygiene and the mechanical pressure exerted on teeth and gums, leading to elemental imbalances. Patients with gingivitis during orthodontic treatment are particularly at risk of developing such imbalances and therefore require timely correction of their mineral metabolism.
Edukasi Hormon Tiroid dan Antitiroid Terhadap Penyakit Gondok di SMK Wirasaba Karawang
Authors: Shalih Mally Ghinan , Rahmawati Marsah , Adam Muhammad Ibnu , Almas Jahra 
Published: October 2024

Penyakit gondok masih menjadi masalah kesehatan global, terutama di daerah yang kekurangan yodium. Gondok yang tidak diobati dapat menyebabkan masalah kesehatan yang berbahaya, seperti gangguan tiroid, gangguan hormonal, kesulitan menelan, serta komplikasi yang lebih parah seperti kanker tiroid. Penyuluhan ini bertujuan untuk membantu siswa mengidentifikasi gejala gondok pada tahap awal, sehingga dengan mengetahui gejala awal para siswa dapat mencegah terjadinya penyakit gondok, siswa harus memahami obat-obatan dan tindakan medis yang diperlukan. Edukasi ini diharapkan dapat membantu para siswa mengambil langkah-langkah dan pemantauan yang tepat untuk mengatasi terjadinya penyakit gondok. Pada penyuluhan kali ini dilakukan di SMK Wirasaba dan digunakan metode pembelajaran partisipatif dimana siswa diberikan pertanyaan kemudian dianalisis secara statistika dan metode mixed-method dimana metode ini merupakan gabungan dari metode kualitatif dan kuantitatif untuk melakukan uji pengolahan data. Setelah dilakukan akumulasi dari nilai Pretest dan Posttest dan dilakukan pengukuran pengetahuan mengenai hormon tiroid dan antitiroid terhadap penyakit gondok pada siswa sebanyak 30 responden pada awalnya rata-rata yang diperoleh 53,50% menjadi 77,57%, hal ini menunjukkan hasil yang naik signifikan setelah peserta diberikan pematerian yaitu mengalami kenaikan sebesar 24,07%, hal ini menunjukan bahwa pengetahuan siswa/i mengenai hormon tiroid dan antitiroid terhadap penyakit gondok meningkat.