bluuebunny/crossref_metadata_2025_split huggingface dataset:
wblE2transcription factor inStreptomyces griseusS4‐7 plays an important role in plant protection
Authors: Cho Hyun Ji , Kwon Young Sang , Kim Da‐Ran , Cho Gyeongjun , Hong Seong Won , Bae Dong‐Won , Kwak Youn‐Sig 
Published: May 2017

AbstractStreptomyces griseusS4‐7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for Fusarium wilt suppressive soils.S. griseusS4‐7 shows specific and pronounced antifungal activity againstFusarium oxysporumf. sp.fragariae. In theStreptomycesgenus, thewhitranscription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production.wblE2function as a regulator has emerged as a new group inwhitranscription factors. In this study, we reveal the involvement of thewblE2transcription factor in the plant‐protection byS. griseusS4‐7. We generated ΔwblE, ΔwblE2, ΔwhiH, and ΔwhmDgene knock‐out mutants, which showed less antifungal activity both in vitro andin planta. Among the mutants,wblE2 mutant failed to protect the strawberry against the Fusarium wilt pathogen. Transcriptome analyses revealed major differences in the regulation of phenylalanine metabolism, polyketide and siderophore biosynthesis between the S4‐7 and thewblE2 mutant. The results contribute to our understanding of the role of streptomyceteswblE2genes in a natural disease suppressing system.
Role of sulfotransferases in resveratrol metabolism in human adipocytes
Authors: Gheldof Nele , Moco Sofia , Chabert Christian , Teav Tony , Barron Denis , Hager Jorg 
Published: May 2017

ScopePolyphenols such as resveratrol received interest for their wide‐ranging biological benefits, including anti‐obesity potential, mimicking effects of caloric restriction with reduced body fat and increased energy expenditure. However, resveratrol is rapidly metabolized, and it is not completely understood which form of resveratrol is responsible for the effects observed within target cells such as adipocytes. Also the role of metabolizing enzymes has not been investigated before.Methods and resultsResveratrol metabolism was evaluated in human adipocytes by UHPLC‐MS at low physiological doses. Resveratrol was found to rapidly metabolize into its sulfated form, while resveratrol glucuronides were undetectable. Only resveratrol, but not its sulfated nor glucuronidated forms had an antilipolytic effect on adipocytes. The metabolizing enzyme responsible for sulfation of polyphenols is SULT1A1, and was found to be upregulated upon adipocyte differentiation. Knocking down SULT1A1 in adipocytes led to decreased resveratrol sulfate and increased resveratrol intra‐ and extracellularly. This lower SULT1A1 activity resulted in an increased antilipolytic effect of resveratrol on adipocytes, as demonstrated by lower glycerol accumulation, which could be attributed to lower activity of the lipolytic protein, perilipin.ConclusionSulfotransferase activity modulates metabolism of resveratrol in adipocytes with potential consequences on bioavailability and thus metabolic action of this polyphenol.
Laser Desorption/Ionisation Mass Spectrometry Imaging of European Yew (Taxus baccata) on Gold Nanoparticle‐enhanced Target
Authors: Arendowski Adrian , Ruman Tomasz 
Published: May 2017

AbstractIntroductionEuropean yew (Taxus baccata) is a plant known to man for centuries as it produces many interesting and important metabolites. These chemical compounds were repeatedly analysed by various analytical techniques, but none of the methods used so far allowed the localisation of the chemical compounds within the tissue and also correlation between plant morphology and its biochemistry.ObjectiveVisualisation of the spatial distribution of yew metabolites with nanoparticle‐based mass spectrometry imaging.MethodologyCompounds occurring on cross‐section of a one‐year yew sprig has been transferred to gold nanoparticle‐enhanced target (AuNPET) by imprinting. The imprint was then subjected to mass spectrometry imaging analysis.ResultsNanoparticle‐enhanced mass spectrometry imaging made it possible to study the distribution of selected compounds in the European yew tissue, including taxanes – terpene alkaloids characteristic for the Taxus genus. Results prove that aspartate, taxinine M, baccatin IV and taxine B are located mainly in the cortex. Taxuspine W was located in the vascular tissue. Maleate was found to be located mainly in the phloem tissue. In contrast, the proton adduct of chlorophyll b was found in the external layer of twigs.ConclusionThe results presented a high correlation between the location of compounds and the morphology of the plant, thus giving the opportunity to see the selected details of chemical structure of the analysed tissue for the first time. Copyright © 2017 John Wiley & Sons, Ltd.
Interference of allelopathic rice with penoxsulam‐resistant barnyardgrass
Authors: Yang Xue‐Fang , Kong Chui‐Hua , Yang Xia , Li Yong‐Feng 
Published: May 2017

AbstractBACKGROUNDDespite increasing knowledge of allelopathic rice interference with barnyardgrass, relatively little is known about its action on herbicide‐resistant barnyardgrass. The incidence of herbicide‐resistant barnyardgrass is escalating in paddy fields. Knowledge of the interference of allelopathic rice with herbicide‐resistant barnyardgrass and the potential mechanisms involved is warranted.RESULTSPenoxsulam‐resistant and ‐susceptible barnyardgrass biotypes were identified and segregated from a putative penoxsulam‐resistant population occurring in paddy fields in China. Allelopathic rice inhibited the growth of barnyardgrass roots more than shoots, regardless of biotype. In particular, there was a stronger inhibition for resistant barnyardgrass than for susceptible barnyardgrass. Allelopathic rice significantly reduced total root length, total root area, maximum root amplitude and maximum root depth in barnyardgrass. Furthermore, the rice allelochemicals tricin and momilactone B inhibited the growth of both resistant and susceptible barnyardgrass. Compared with root contact, root segregation significantly increased inhibition of barnyardgrass with an increase in rice allelochemicals. Root exudates from barnyardgrass induced the production of rice allelochemicals, but the effect of susceptible barnyardgrass was much stronger than that of resistant barnyardgrass.CONCLUSIONAllelopathic rice can interfere with the growth of penoxsulam‐resistant barnyardgrass through allelochemical‐mediated root interactions. This type of allelopathic interference may provide a non‐herbicidal alternative for herbicide‐resistant weed management in paddy systems. © 2017 Society of Chemical Industry
Clinical performance and survivorship of navigated floating platform mobile‐bearing total knee arthroplasty: A minimum 10‐year follow‐up
Authors: Kim Sang‐Min , Seo Jai‐Gon , Lim Seung‐Jae , Lim Hyung‐Tae , Cho Chang‐Ho , Moon Young‐Wan 
Published: May 2017

AbstractBackgroundThe purpose of this study is to report the outcome of navigation‐assisted cruciate‐retaining total knee arthroplasty (TKA) using one type of cemented, second‐generation, floating‐platform (FP), mobile‐bearing system.MethodsForty‐two patients who underwent cruciate retaining TKAs using e.motion‐FP prostheses under navigational guidance were retrospectively reviewed. The preoperative diagnosis was osteoarthritis in all knees except one rheumatoid arthritis. The mean follow‐up was 132.0 months (range, 120–140 months) and the mean age was 64.0 ± 4.7 years (range, 51–76 years) at the time of index surgery. Clinical and radiographic results as well as mechanical survival rate of this type of prosthesis were investigated at a minimum follow‐up of 10 years.ResultsThe mean mechanical femorotibial angle was improved from 11.7° ± 3.3° preoperatively to 1.4° ± 1.7° at the latest follow‐up. No prosthesis‐related complications occurred. One knee underwent open debridement due to superficial infection at 5 weeks after surgery and the other knee experienced a periprosthetic fracture around the proximal tibia, which was successfully healed after open reduction and internal fixation.ConclusionsThe e.motion‐floating platform mobile‐bearing design yielded satisfactory long‐term durability and implant performance under navigational guidance.
Technical feasibility and safety of image‐guided parieto‐occipital ventricular catheter placement with the assistance of a wearable head‐up display
Authors: Yoon Jang W. , Chen Robert E. , ReFaey Karim , Diaz Roberto J. , Reimer Ronald , Komotar Ricardo J. , Quinones‐Hinojosa Alfredo , Brown Benjamin L. , Wharen Robert E. 
Published: May 2017

AbstractBackgroundWearable technology is growing in popularity as a result of its ability to interface with normal human movement and function.MethodsUsing proprietary hardware and software, neuronavigation images were captured and transferred wirelessly via a password‐encrypted network to the head‐up display. The operating surgeon wore a loupe‐mounted wearable head‐up display during image‐guided parieto‐occipital ventriculoperitoneal shunt placement in two patients.ResultsThe shunt placement was completed successfully without complications. The tip of the catheter ended well within the ventricles away from the ventricular wall. The wearable device allowed for continuous monitoring of neuronavigation images in the right upper corner of the surgeon's visual field without the need for the surgeon to turn his head to view the monitors.ConclusionsThe adaptable nature of this proposed system permits the display of video data to the operating surgeon without diverting attention away from the operative task. This technology has the potential to enhance image‐guided procedures.
On a Cohesive Set of Requirements Engineering Terms
Authors: Ryan Michael J. , Wheatcraft Louis S. 
Published: May 2017

ABSTRACTThe purpose of a requirement expression is to transform the needs of various entities into a formal language such that the intent is clearly understood by all involved. Although many sources provide definitions of the terms associated with a requirement expression, few contain guidance as to the process of developing a requirement, there are only occasional agreements on common definitions, and the defined terms are too narrowly focused to be useful across the full requirements engineering domain. This paper develops a cohesive set of definitions of the terms associated with a requirement expression. First, a framework for the transformation of needs into requirements is discussed and existing definitions are presented from the major relevant sources. These definitions are then analyzed and an integrated set of definitions is developed for the key terms associated with a requirements expression: entity; need; requirement expression; requirement statement; characteristics of a well‐formed requirement statement and a well‐formed set of requirements; and requirement attributes that, along with the requirement statement itself, comprise a well‐formed requirement expression.
Development of a modified stochastic subspace identification method for rapid structural assessment of in‐service utility‐scale wind turbine towers
Authors: Dai Kaoshan , Wang Ying , Huang Yichao , Zhu Weidong , Xu Yongfeng 
Published: May 2017

AbstractThe strong drive to harness wind energy has recently led to rapid growth of wind farm construction. Wind turbine towers with increased sizes and flexibility experience large vibrations. Structural health monitoring of wind turbines is proposed in the wind energy industry to ensure their proper performance and save maintenance costs. This study proposes a system identification method for vibration‐based structural assessment of wind turbine towers. This method developed based on the stochastic subspace identification method can identify modal parameters of structures in operating conditions with harmonic components in excitations. It benefits wind turbine tower structural health assessment because classical operational modal analysis methods can fail as periodic rotation excitation from a turbine introduces harmonic disturbance to tower structure response data. The effectiveness, accuracy and robustness of the proposed method were numerically investigated and verified through a lumped‐mass system model. The method was then applied to an in‐service utility‐scale wind turbine tower. The field testing campaign and modal parameter identification as well as structural assessment results were presented. Copyright © 2017 John Wiley & Sons, Ltd.
Worry and cognitive control predict course trajectories of anxiety in older adults with late-life depression
Authors: Spinhoven P. , van der Veen D.C. , Voshaar R.C. Oude , Comijs H.C. 
Published: May 2017

AbstractBackground:Many older adults with depressive disorder manifest anxious distress. This longitudinal study examines the predictive value of worry as a maladaptive cognitive emotion regulation strategy, and resources necessary for successful emotion regulation (i.e., cognitive control and resting heart rate variability [HRV]) for the course of anxiety symptoms in depressed older adults. Moreover, it examines whether these emotion regulation variables moderate the impact of negative life events on severity of anxiety symptoms.Methods:Data of 378 depressed older adults (CIDI) between 60 and 93 years (of whom 144 [41%] had a comorbid anxiety disorder) from the Netherlands Study of Depression in Older Adults (NESDO) were used. Latent Growth Mixture Modeling was used to identify different course trajectories of six-months BAI scores. Univariable and multivariable longitudinal associations of worry, cognitive control and HRV with symptom course trajectories were assessed.Results:We identified a course trajectory with low and improving symptoms (57.9%), a course trajectory with moderate and persistent symptoms (33.5%), and a course trajectory with severe and persistent anxiety symptoms (8.6%). Higher levels of worry and lower levels of cognitive control predicted persistent and severe levels of anxiety symptoms independent of presence of anxiety disorder. However, worry, cognitive control and HRV did not moderate the impact of negative life events on anxiety severity.Conclusions:Worry may be an important and malleable risk factor for persistence of anxiety symptoms in depressed older adults. Given the high prevalence of anxious depression in older adults, modifying worry may constitute a viable venue for alleviating anxiety levels.
Oncology ostomized patients’ perception regarding sexual relationship as an important dimension in quality of life
Authors: Kimura Cristilene Akiko , Guilhem Dirce Bellezi , Kamada Ivone , de Abreu Breno Silva , Fortes Renata Costa 
Published: May 2017

AbstractIntroduction For ostomized oncological patients, the physical body alterations affects the quality of life, as the changes in the self-concept are factors that directly affect the sexual life.Objective To analyze the perceptions of ostomized men due to intestinal cancer regarding sexual relations as an important dimension of quality of life, treated at the Ambulatory Care Program for Ostomized Patients of the Health Secretariat of the Federal District, Brazil.Methods Epidemiological-based study, of the analytical type, with a cross-sectional descriptive design, with quantitative and qualitative approach considering the content analysis. The convenience sample included 56 participants. Sociodemographic, clinical, and the WHOQOL-BREF questionnaires were used, as well as an individual interview. Data were analyzed by Microsoft Office Excel 2010 and SPSS 20.0 software. Statistical significance was set at 5%.Results The Physical, Social Relations and Environment Domains are correlated with the mean score, statistical significance (p < 0.0001), and the content analysis resulted in five categories: Ostomy, Self-Care, Acceptance, Self-concept, and Companionship.Conclusion Sexuality should be considered as a process of daily living of ostomized individuals due to intestinal cancer.
A prospective study on tubercular fistula in ano and its management
Authors: Sahu Manoranjan , Mishra Jai Krishna , Sharma Ashish , Fatmi Uzma 
Published: May 2017

AbstractObjective Tuberculosis fistula in ano, though less encountered, is an important clinical entity in developing countries like India. Diagnosis of TB fistula is a challenge despite of advances in diagnostic modalities and it depends upon both local and systemic clinical presentation. This prospective study aimed at to substantiate the importance of clinical diagnosis as well as medical management of tubercular fistula by antitubercular drugs.Methods and results 25 patients of fistula in ano suspected to be of tubercular origin underwent histopathology of fistulous tracks and an 8 week therapeutic trial of antitubercular treatment after getting an informed consent. Though biopsy showed positive evidence of tubercular pathology only in 52% cases, therapeutic trial showed improvement in local and systemic features in 23 (92%) cases. Of these 23 cases, 3 were cured after 18 months of anti tubercular treatment and 18 showed cure after 24 months of anti tubercular treatment while 2 cases withdrew from the study at 12 and 14 months respectively due to adverse drug reactions though their fistulous symptoms were relieved.Conclusion Meticulous clinical evaluation plays a vital role in diagnosis of tubercular fistula in addition to other diagnostic methods. Anti tubercular treatment is the mainstay of treatment in tubercular fistula with a minimum duration of 18–24 months owing to the recurrent and relapsing nature of disease.
Heavy metal contamination of animal feedstuffs – a new survey
Authors: Elliott S. , Frio A. , Jarman T. 
Published: May 2017

SummaryContamination of feedstuffs and ingredients with heavy metals poses a major problem for animal health and the transmission of toxic substances within the human food chain, as these elements can be accumulated into meat, egg and milk products. This paper reports on the levels of the three metals, arsenic, cadmium and lead, that exceed EU permitted levels, within premixes and complete feeds (total of 3417 samples) destined for a variety of animal species and taken from different countries during the period 2009–2016. For this period, 20% of all samples were contaminated at levels above the EU limit. In complete feed, ruminants were at the highest risk of exposure with 47% of samples being contaminated. Lead posed the major risk for minerals and cadmium posed the highest risk for premixes during the sampling period. When compared by country, contamination levels varied widely, with the highest contamination determined for Canada with 50% of samples being above the EU limit. To mitigate problems with heavy metals in animal feeds, due diligence in terms of sampling and testing is a necessary tool to evaluate level of risk. Research has shown that trace minerals can be sources of these heavy metals so the industry should be more diligent regarding their provider's quality guarantees. In addition, feeds identified with contamination should not be fed to animals to ensure that these heavy metal toxins are not passed into edible food products, thus providing safety in the food chain.
What Determines Exports of Luxury Products? The Case of Cognac
Authors: Bouët Antoine , Emlinger Charlotte , Lamani Viola 
Published: May 2017

AbstractThis paper analyzes the determinants of Cognac brandy exports using a unique database on Cognac shipments to more than 140 destinations between 1996 and 2013. We use this database to construct descriptive statistics concerning the evolution of Cognac exports during this period. We also construct a database of protectionist policies that affect worldwide Cognac exports. We analyze the determinants of Cognac exports and base our empirical strategy on Heckman's (1979) procedure. We estimate successively the impact of geographical, demand and policy factors on the extensive margin of trade and the intensive margin of trade. We also control for the possibility of an endogeneity bias on the probability of trade. We show that i) as with other luxury products, the elasticity of Cognac exports to distance is negative, significant, and relatively small, while the elasticity to gross domestic product (GDP) is positive, significant, and relatively large; and ii) average customs duties do not have a significant impact on the intensive margin but significantly and positively affect the probability of trade. We discuss this last result and correct the endogeneity bias using tax revenues of importing countries in percentage of GDP as an instrument. (JEL Classifications: F10, F14)
Do Good Things Come in Small Packages? Bottle Size Effects on Willingness to Pay for Pomegranate Wine and Grape Wine
Authors: Drichoutis Andreas C. , Klonaris Stathis , Papoutsi Georgia S. 
Published: May 2017

AbstractWe evaluate the claim that bottle size formats signal quality changes by performing a controlled laboratory experiment in which we simultaneously auction two different sweet wines: a pomegranate wine and a grape wine. We vary the size of the bottle from 500 mL to 750 mL between participants, but we keep the amount of wine constant across the bottle sizes. We also explore the effect of expectations for the wines, blind tasting, and information about wine attributes on people's willingness to pay (WTP). For both wines, we find evidence consistent with diminishing marginal utility; for the pomegranate wine, we find a premium for the smaller bottle size, which is consistent with changes in the wine's perceived quality. We also find that information is adequate in offsetting the negative effect of the tasting treatment. (JEL Classifications: C23, C24, C91, D12, M31)
A note on scribal error
Authors: Wevers John Wm 
Published: May 2017

0. The Purpose of this short study is to show that phonetic rather than graphemic considerations predominate in scribal errors in Greek Biblical manuscripts and to suggest an explanation for this phenomenon.1. In preparing my critical edition of the Greek Genesis, all available extant mss of Genesis prior to the 16th century were collated, the only exceptions being a 15th-century ms which was unavailable to the Göttingen Academy, and two other 15th-century mss which proved to be copies of mss already collated. The mss collated constituted 9 uncial texts dating from the 3rd to the 7th centuries and 98 minuscules dating from the 9th to the 15th centuries. Also collated were 32 papyri ranging from ca. 50 Bc (a tiny fragment containing only a few letters) to the 9th century. Three of these papyri are substantial, the so-called Berlin Genesis (late 3rd century) and the Chester Beatty Papyri IV and V (4th and late 3rd centuries respectively).
A micro-method for the quantitative estimation of rennets and other proteolytic enzymes
Authors: Lawrence R. C. , Sanderson W. B. 
Published: May 2017

SummaryAn agar diffusion slide assay using a thin layer of calcium caseinate has been developed as a micro-method for the quantitative study of calf rennet and other proteolytic enzymes. The number and width of the precipitation zones formed as a result of proteolytic activity depended upon the enzyme used, the concentration of calcium ion, pH and temperature. The assay was highly reproducible and particularly suitable for the measurement of large numbers of samples. It was considerably more sensitive than the milk clotting technique and, in general, more sensitive than other standard methods for measuring proteolytic activity.
Weed taints in diary produce. I. Lepidium taint
Authors: Park R. J. 
Published: May 2017

SummaryA re-investigation has been made of the nature of the tainting substances present in butterfat possessing the weed taint which arises through the ingestion of Lepidium spp. by dairy cattle. Some 0.5 ppm of skatole were isolated from Lepidiumtainted butterfat, along with 0.3 ppm indole. Flavour evaluation tests demonstrated that the skatole was principally responsible for the flavour defect, somewhat modifying the conclusions arrived at by earlier investigators.