bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling of Hot and Cold Storage Silos With a Moving Packed-Bed Shell-and-Plate Heat Exchanger in a Particle Based Concentrated Solar Power System
Authors: Marton Matthew , Nanclares Juan Camilo , Ordonez Juan C. , Arias Jesus , Haynes Comas 
Published: September 2024

Abstract
The next generation of Concentrated Solar Power (CSP) systems is envisioned to use solid particles for heat collection and storage. This paper presents a computational model for a particle storage silo and for a heat exchanger in which hot solid particles exchange heat with super critical carbon dioxide that serves as the working fluid for the CSP power block. A novel storage silo model is presented in which the particles are modeled as a quasi-1D fin. All heat conduction through particles is calculated using the ZBS model for particle heat conduction. Silo geometry is based upon G3P3 requirements for thermal storage. The heat exchanger model is compared against previously completed models. Temperatures for the stored particles and insulation layers in a quasi steady-state configuration where the particles are emptied and refilled every 10 hours over a 100 hour period.
Genome sequence of
Sulfurimonas
sp. C5, a potential chemolithoautotrophic, sulfur-oxidizing bacterium isolated from a mangrove sediment
Authors: Yuan Qing , Wang Shufang , Wang ShaSha , Zhong Yangsheng , Jiang Lijing 
Published: September 2024

ABSTRACT

Sulfurimonas
sp. strain C5 (MCCC 1A19556) is a strain with potential chemolithoautotrophic sulfur-oxidizing function, which is isolated from a mangrove sediment sample collected from Quanzhou Bay, Fujian Province, China. This report describes the genome sequence of strain C5, which possesses the gene sets for the sulfur-oxidizing pathway.
Genome sequence of dengue virus serotype 1 isolated from a pediatric patient enrolled in an Ivermectin trial in Thailand
Authors: Sayboonruan Preeyanuch , Songjaeng Adisak , Chin-Inmanu Kwanrutai , Proykhunthod Phitchapha , Prayongkul Dararat , Poraha Ranyikar , Mairiang Dumrong , Lapphra Keswadee , Chokephaibulkit Kulkanya , Punyadee Nuntaya , Avirutnan Panisadee 
Published: September 2024

ABSTRACT

We sequenced the genome of dengue virus serotype 1 (DENV1) strain 4101301, isolated from a child with dengue fever in Thailand and cultured in C6/36 mosquito cells. These data are crucial for studying DENV1’s genetic diversity, evolution, and epidemiology and advancing the knowledge for developing antiviral drugs and vaccines targeting DENV.
Genome sequences of
Pseudomonas
sp. strain FFPRI_1 and
Photorhabdus temperata
strain FFPRI_2, isolated from the entomopathogenic nematode,
Heterorhabditis megidis
Authors: Takatsuka Jun , Maehara Noritoshi 
Published: September 2024

ABSTRACT

The complete genome sequences of two bacteria isolated from an entomopathogenic nematode are reported. The genome sizes of
Pseudomonas
sp. strain FFPRI_1 and
Photorhabdus temperata
strain FFPRI_2 are 7,521,420 bp long with 63% G+C content and 5,483,510 bp long with 43.8% G+C content, respectively.
Two distinct
Dehalobacter
metagenome-assembled genomes from anaerobic chloroform and dichloromethane degrading consortia
Authors: Bulka Olivia , Edwards Elizabeth A. 
Published: September 2024

ABSTRACT

Here we present two metagenomes and two
Dehalobacter
metagenome-assembled genomes from subcultures of an anaerobic chloroform and dichloromethane degrading microbial community used for bioremediation. Our objective was to assemble and curate the genome(s) of
Dehalobacter
, key biodegraders in the culture, through repeated sequencing and joint assembly with previous datasets.
Gut microbiota-derived gamma-aminobutyric acid improves host appetite by inhibiting satiety hormone secretion
Authors: Li Shouren , Liu Mengqi , Han Yao , Liu Cong , Cao Shixi , Cui Yalei , Zhu Xiaoyan , Wang Zhichang , Liu Boshuai , Shi Yinghua 
Published: September 2024

ABSTRACT

Globally, appetite disorders have become an increasingly prominent public health issue. While short-term appetite loss may seem relatively harmless, prolonged instances can lead to serious physical and mental damage. In recent years, numerous studies have highlighted the significant role of the “microbiota-gut-brain” axis in the regulation of feeding behavior in organisms, suggesting that targeting the gut microbiota may be a novel therapeutic strategy for appetite disorders. However, the molecular mechanisms through which the gut microbiota mediates the increase in host appetite and the causal relationship between the two remain unclear. Based on this, we conducted 16S rRNA sequencing to analyze the gut microbiota of rabbits with high and low feed intake, followed by fecal microbiota transplantation (FMT) and metabolite gavage experiments to elucidate the underlying mechanisms. Our research indicates that the high feed intake group exhibited significant enrichment of the
g__Bacteroides
and gamma-aminobutyric acid (GABA), and intragastric administration of GABA effectively promoted the host’s feeding behavior. The underlying mechanism involves GABA derived from the gut microbiota inhibiting the secretion of satiety hormones to enhance the host’s feeding behavior. Furthermore, the results of FMT suggest that differences in gut microbiota composition may be a contributing factor to varying levels of feed intake in the host. In conclusion, these findings emphasize the role of the gut microbiota-derived GABA, in increasing host feed intake, offering a new target for the treatment of appetite disorders from the perspective of gut microbiota.

IMPORTANCE
The incidence of anorexia is rapidly increasing and has become a global burden. Gut microbiota can participate in the regulation of host feeding behavior, yet the molecular mechanisms through which the gut microbiota mediates the increase in host appetite and the causal relationship between them remain unclear. In this study, we utilized 16S rRNA sequencing to investigate the composition of the gut microbiota in rabbits with varying levels of feed intake and employed fecal microbiota transplantation and gastric infusion experiments with gamma-aminobutyric acid (GABA) to elucidate the potential mechanisms involved. GABA derived from the gut microbiota can effectively enhance the host’s feeding behavior by inhibiting the secretion of satiety hormones. This discovery underscores the pivotal role of the gut microbiota in modulating host appetite, offering novel research avenues and therapeutic targets for appetite disorders.
A conserved two-component system senses intracellular iron levels and regulates redox balance in
Mycobacterium
spp.
Authors: Yadav Rahul , Saini Deepak Kumar 
Published: September 2024

ABSTRACT

For bacteria, an intricate coordination between sensing and regulating iron levels and managing oxidative stress is required as their levels are tightly interlinked. While various oxidative stress and heme-based redox sensors have been reported for both pathogenic and non-pathogenic bacteria, the mechanisms governing the modulation of intracellular iron levels in response to changes in redox status remain unclear. In this study, a gene-inactivated strain of mycobacterial sensor kinase PdtaS showed dysregulated expression of genes associated with iron metabolism, including Fe-S clusters, NADH dehydrogenases, and iron uptake. The strain showed poor growth in nutrient-limiting conditions, a defect rescuable by heme but not by Fe
3+
supplementation. This observation was associated with the PAS domain of the PdtaS sensor kinase. Biochemical and biophysical experiments established heme-binding to the PAS domain and its inhibitory effect on PdtaS auto-kinase activity, suggesting that the absence of heme induces activation of this sensor kinase. Interestingly, despite having an endogenous heme biosynthetic pathway or even external heme supplementation, the ∆
pdtaS
mutant exhibited persistent low intracellular iron levels concomitant with elevated oxidative stress. Antioxidant supplementation mitigated growth defects, emphasizing the link between oxidative stress, intracellular iron levels, and PdtaS activity. RNA-IP identified key targets associated with redox homeostasis and iron metabolism as targets of the PdtaR response regulator. The study proposes a novel role for the PdtaS-PdtaR TCS in sensing heme, regulation of intracellular iron levels, and redox balance.

IMPORTANCE
The research article investigates the intricate interplay between bacteria’s ability to take and utilize iron without inducing excess iron’s toxic effects, including oxidative stress. The study shows that bacteria achieve this by sensing intracellular iron available as heme through a sensory protein PdtaS, which turns off when heme is in excess and prevents iron uptake and iron efflux. The process shields bacteria from generating Fe-dependent free radicals and allows it to maintain viability. The absence of sensor kinase abrogates all these processes, increasing bacteria susceptibility to ROS and thereby slowing growth. This feature of the sensor kinase PdtaS makes it an attractive co-therapeutic target for tuberculosis therapy, where its inhibition will prevent iron uptake, even in the presence of low iron, thereby halting bacterial proliferation.
Seasonal succession of endophyte and the association with active ingredients in
Rheum palmatum
Authors: Li Ni , Mao YiFan , Huang YaLi , Zhang LingXuan , Hou Lu , Liu XiaoJun , Du YaRong , Chen DaWei , Sun Kun 
Published: September 2024

ABSTRACT

The endophyte is closely related to medicinal plant growth and development, stress resistance, and active ingredients’ accumulation. However, a seasonal succession of endophytes and the association with active ingredients is still unclear. In this study, we used high-throughput sequencing methods to compare the endophyte diversity of
Rheum palmatum
under different seasons and analyze the association between endophytes and five active ingredients. The results show that the diversity of endophytic fungi increased and then decreased, while bacterial diversity increased with the change of season. Community composition showed that the dominant genera of endophytic fungi were different under the different seasons, while the dominant genera of endophytic bacteria were
Delftia
. Analysis of co-occurrence network maps showed that the connectivity and complexity of endophytic fungi and bacterial networks decreased with the change of season. Spearman analysis indicated that the active ingredients of
R. palmatum
were significantly positive correlation with genera of endophytic fungi (
Chalara
). FUNGuild and PICRUSt predictive analysis indicated that the function of endophytic fungi and bacteria, respectively, were symbiotroph and metabolism, and relative abundances were different under the different seasons. Our results help elucidate the mechanism of medicinal plant–endophyte interaction.

IMPORTANCE

Through the investigation of the seasonal succession of endophytes and the association with active ingredients in
Rheum palmatum
, we found that the diversity and composition of endophytes in
R. palmatum
exhibited seasonal dynamics, and the active ingredients of
R. palmatum
showed a significantly positive correlation with the genus of endophytic fungi (
Chalara
). Our results may lay a foundation for understanding the interaction mechanism of endophyte and medicinal plant, and can also provide a theoretical basis for sustainable production of medicinal plants.
Wastewater-based intestinal protozoa monitoring in Shanghai, China
Authors: Jiang Yanyan , Yuan Zhongying , Wang Yaxue , Zhang Jing , Shen Yujuan , Cao Jianping 
Published: September 2024

ABSTRACT

Intestinal protozoa
Cryptosporidium
spp.,
Giardia duodenalis
, and
Enterocytozoon bieneusi
have been implicated in serious waterborne outbreaks worldwide. Wastewater-based epidemiology (WBE) is a promising approach for evaluating the disease prevalence in a catchment population in that it monitors the contamination level of the intestinal pathogens in wastewater. We collected 48 urban wastewater samples (24 from influents and 24 from effluents) from the Yangpu Wastewater Treatment Plant (YPWTP) in Shanghai, China. We identified
Cryptosporidium
spp.,
G. duodenalis
, and
E. bieneusi
by nested polymerase chain reaction (PCR) amplification.
Cryptosporidium hominis
and subtype IdA14 were identified in two samples by analyzing the sequences of small subunit ribosomal RNA (SSU rRNA) and 60–kDa glycoprotein (
gp60
) genes, respectively. The
G. duodenalis
sub-assemblage AII (
n
= 8) and assemblage C (
n
= 4) in 12 samples were determined by analyzing triosephosphate isomerase (
tpi
) gene sequences. The
E. bieneusi
genotype A was identified in one sample by analyzing the sequence of the internal transcribed spacer (ITS) region of the rRNA gene. These findings suggest that improving wastewater treatment and monitoring the virility of pathogens in effluents is critical. We observed similar prevalence and genotypes/subtypes of the three intestinal protozoa in our wastewater samples as those reported in previous studies, providing evidence that WBE can be used as an effective epidemic management tool.

IMPORTANCE

Cryptosporidium
spp.,
Giardia duodenalis,
and
Enterocytozoon bieneusi
are common intestinal protozoa causing diarrhea. The infective oocysts, cysts, and spores released in feces can survive in different environments, including multiple types of water bodies. Humans can acquire these intestinal protozoan infections
via
the fecal-oral route as in waterborne transmission. Wastewater-based epidemiology can rapidly and reliably detect and monitor the emergence and spread of waterborne diseases. We detected
Cryptosporidium
spp.,
G. duodenalis,
and
E. bieneusi
in a wastewater treatment plant in Shanghai, China, reflecting the occurrence and genetic characterizations of the three intestinal pathogens from community members served by the wastewater treatment plant.
Back pain Knowledge and beliefs Survey (BacKS): development and assessment of measurement properties
Authors: Corrêa Leticia Amaral , Hancock Mark , Mathieson Stephanie , Verhagen Arianne , Darlow Ben , Hodges Paul William , French Simon 
Published: September 2024

Objective
To develop and evaluate a new patient-reported outcome measure (PROM) to assess people’s knowledge and beliefs about low back pain.

Methods
This study followed the COnsensus-based Standards for the selection of health Measurement INstruments guidelines. An 18-item preliminary version of the Back pain Knowledge and beliefs Survey (BacKS) was generated based on evidence-based key messages and current clinical guidelines for low back pain. Four items were added following input from three consumers and seven experts. Focus groups (n=9) confirmed content validity. The 22-item version was completed by 258 Australian-based adults (>18 years) with self-reported low back pain. A follow-up survey was sent 1 week later. The following measurement properties were evaluated to produce, and then assess the final version of BacKS: structural validity (exploratory factor analysis); internal consistency (Cronbach’s alpha); test–retest reliability (intraclass correlation coefficient); measurement error (Smallest Detectable Change); construct validity (hypothesis tested: moderate positive Pearson correlation between BacKS and Back Beliefs Questionnaire); plus, interpretability and feasibility.

Results
The final BacKS comprised 20 items with a 2-factor structure (biomedical factor: 9 items, score ranging from 9 to 45, and self-care factor: 11 items, score ranging from 11 to 55). Internal consistency and reliability were adequate (>0.70) for each factor. Smallest detectable change was 4.4 (biomedical factor) and 7.0 (self-care factor). Our construct validity hypothesis was confirmed (Pearson correlation=0.53). No floor or ceiling effects were detected.

Conclusion
The BacKS is a valid, reliable and feasible PROM to measure knowledge and beliefs about low back pain in clinical practice and research settings.
Human resources for health: a framework synthesis to put health workers at the centre of healthcare
Authors: Nwankwo Ogonna N O , Auer Christian , Oyo-Ita Angela , Eyers John , Wyss Kaspar , Fink Günther , Bosch-Capblanch Xavier 
Published: September 2024

BackgroundHuman resources are a key determinant for the quality of healthcare and health outcomes. Several human resource management approaches or practices have been proposed and implemented to better understand and address health workers’ challenges with mixed results particularly in low- and middle-income countries (LMICs). The aim of this framework synthesis was to review the human resources frameworks commonly available to address human resources for health issues in LMIC.MethodsWe searched studies in Medline, Embase, CAB Global Health, CINAHL (EBSCO) and WHO global Index Medicus up to 2021. We included studies that provided frameworks to tackle human resources for health issues, especially for LMICs. We synthesised the findings using a framework and thematic synthesis methods.ResultsThe search identified 8574 studies, out of which 17 were included in our analysis. The common elements of different frameworks are (in descending order of frequency): (1) functional roles of health workers; (2) health workforce performance outcomes; (3) human resource management practises and levers; (4) health system outcomes; (5) contextual/cross-cutting issues; (6) population health outcomes and (7) the humanness of health workers. All frameworks directly or indirectly considered themes around the functional roles of health workers and on the outcomes of health workforce activities, while themes concerning the humanness of health workers were least represented. We propose a synthesised Human-Centred Health Workforce Framework.ConclusionsSeveral frameworks exist providing different recurring thematic areas for addressing human resources for health issues in LMIC. Frameworks have predominantly functional or instrumental dimensions and much less consideration of the humanness of health workers. The paradigms used in policy making, development and funding may compromise the effectiveness of strategies to address human resources challenges in LMIC. We propose a comprehensive human resources for health framework to address these pitfalls.
Neurological manifestations in adult patients with the m.3243A>G variant in mitochondrial DNA
Authors: Majamaa Kari , Kärppä Mikko , Moilanen Jukka S 
Published: September 2024

BackgroundThe m.3243A>G variant in mitochondrial DNA (mtDNA) is the most common cause of the MELAS (Mitochondrial encephalopathy, lactic acidosis and stroke-like episodes) syndrome usually commencing in childhood or adolescence. In adults, the variant presents with versatile and mostly neurological phenotypes, but MELAS may not be common.ObjectiveTo examine the frequency of phenotypes in adults with m.3243A>G in a population-based cohort and in a meta-analysis of reported case series.MethodsWe clinically examined 51 adult patients with m.3243A>G to determine the frequency of phenotypes and to analyse the contribution of variant heteroplasmy, age, sex and mtDNA haplogroup to the phenotypes. The frequencies of neurological features were also assessed in a meta-analysis on 25 published case series reporting 1314 patients.ResultsSensorineural hearing impairment (HI), cognitive impairment and myopathy were the most common manifestations, whereas stroke-like episodes were infrequent. Variant heteroplasmy and age were only modest predictors of the phenotypes, although heteroplasmy correlated significantly with disability and Kaplan-Meier analysis showed progression of phenotypes with age. Male sex predicted more severe disability, whereas haplogroup UK was associated with no significant disability. Meta-analysis revealed substantial heterogeneity of phenotype frequencies and preferential inclusion of the MELAS phenotype.DiscussionIn adult patients with m.3243A>G sensorineural HI, cognitive impairment and myopathy are common manifestations with lifetime prevalences approaching unity. Stroke-like episodes are rare. Variant heteroplasmy, age, sex and mtDNA haplogroup contribute to the severity of the disease. Meta-analysis provided a solid estimate of the various neurological symptoms in adults with m.3243A>G.
Environmental degradation, climate change and health from the perspective of Brazilian Indigenous stakeholders: a qualitative study
Authors: Grande Antonio José , Dias Ieda M A V , Jardim Paulo T C , Aparecida Vieira Machado Alessandra , Soratto Jacks , da Rosa Maria Inês , Ceretta Luciane Bisognin , Zourntos Xanthi , Suares Regeane Oliveira , Harding Seeromanie 
Published: September 2024

BackgroundThe WHO identifies climate change as the most significant threat to global health systems. Indigenous peoples, whose lives are deeply intertwined with nature, are particularly vulnerable to the impacts of these changes.ObjectiveThis study aimed to understand the perspectives of Indigenous stakeholders and public services managers on the interconnectedness of climate change and Indigenous health.DesignA qualitative study with 22 Indigenous stakeholders and public service managers on climate change and perceived impact on Indigenous health.Setting and participantsIndigenous stakeholders and public service managers on climate change and perceived impact on Indigenous health from Brazil. Data was collected through interviews incorporating two vignette videos depicting environmental and health scenarios. Thematic content analysis was used to analyse the data.ResultsThe analytical process yielded six subcategories that were further grouped into three overarching thematic macro-categories: environmental degradation and climate change in the context of Indigenous peoples; environment, vulnerability and impact on Indigenous mental health; and actions and public health policies for Indigenous peoples.ConclusionThe perspectives of Indigenous stakeholders and public service managers on the interconnectedness of climate change and Indigenous health were deeply entrenched in their lived experiences of loss of their lands from deforestation and environmental degradation. They argued strongly for the strengthening of public health policies aimed at the Indigenous peoples, to face many challenges, especially suicide, and to have a voice in decision-making. A sensitive approach that values Indigenous peoples' connections with nature is fundamental to promote their health and well-being.