bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2
Authors: Wu Liang , Song Wen-yan , Xie Ya , Hu Lin-li , Hou Xiao-man , Wang Rui , Gao Yang , Zhang Jing-na , Zhang Le , Li Wei-wei , Zhu Cheng , Gao Zhi-ying , Sun Ying-pu 
Published: January 2018

AbstractPre-eclampsia is a pregnancy-related disease that may cause maternal, neonatal and fetal morbidity and mortality and exists in 3–5% of pregnancies worldwide. The discovery of dysregulated microRNAs and their roles in placental development has provided a new avenue for elucidating the mechanism involved in this pregnancy-specific disorder. Here, the roles of human miR-181a-5p, a microRNA that is increased in both the plasma and placenta of severe pre-eclamptic patients, in invasion and migration of trophoblasts were investigated. Ectopic-expression of miR-181a-5p impaired the invasion and migration of HTR-8/SVneo cells, whereas miR-181a-5p inhibition had the opposite effects. IGF2BP2, which harbors a highly conserved miR-181a-5p-binding site within its 3ʹ-UTR, was identified to be directly inhibited by miR-181a-5p. Moreover, siRNAs targeting IGF2BP2 imitated the effects of overexpressed miR-181a-5p on HTR-8/SVneo cell invasion and migration, whereas restoring IGF2BP2 expression by overexpressing a plasmid encoding IGF2BP2 partially reversed the studied inhibitory functions of miR-181a-5p. Thus, we demonstrated here that miR-181a-5p suppresses the invasion and migration of cytotrophoblasts, and its inhibitory effects were at least partially mediated by the suppression of IGF2BP2 expression, thus shedding new light on the roles of miR-181a-5p in the pathogenesis of severe pre-eclampsia.
Plumbagin inhibits the proliferation and survival of esophageal cancer cells by blocking STAT3-PLK1-AKT signaling
Authors: Cao Ying-Ya , Yu Jing , Liu Ting-Ting , Yang Kai-Xia , Yang Li-Yan , Chen Qun , Shi Feng , Hao Jia-Jie , Cai Yan , Wang Ming-Rong , Lu Wei-Hua , Zhang Yu 
Published: January 2018

AbstractEsophageal squamous cell carcinoma (ESCC) is one of the deadliest cancers, and it requires novel treatment approaches and effective drugs. In the present study, we found that treatment with plumbagin, a natural compound, reduced proliferation and survival of the KYSE150 and KYSE450 ESCC cell lines in a dose-dependent manner in vitro. The drug also effectively inhibited the viability of primary ESCC cells from fresh biopsy specimens. Furthermore, plumbagin-induced mitotic arrest and massive apoptosis in ESCC cells. Notably, the drug significantly suppressed the colony formation capacity of ESCC cells in vitro and the growth of KYSE150 xenograft tumors in vivo. At the molecular level, we found that exposure to plumbagin decreased both polo-like kinase 1 (PLK1) and phosphorylated protein kinase B (p-AKT) expression in both ESCC cell lines. Enforced PLK1 expression in ESCC cells not only markedly rescued cells from plumbagin-induced apoptosis and proliferation inhibition but also restored the impaired AKT activity. Furthermore, signal transducer and activator of transcription 3 (STAT3), a transcription factor of PLK1, was also inactivated in plumbagin-treated ESCC cells; however, the overexpression of a constitutively activated STAT3 mutant, STAT3C, reinstated the plumbagin-elicited blockade of PLK1-AKT signaling in ESCC cells. Taken together, these findings indicate that plumbagin inhibits proliferation and potentiates apoptosis in human ESCC cells in vitro and in vivo. Plumbagin may exert these antitumor effects by abrogating STAT3-PLK1-AKT signaling, which suggests that plumbagin may be a novel, promising anticancer agent for the treatment of ESCC.
Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans
Authors: Seeleuthner Yoann , Mondy Samuel , Lombard Vincent , Carradec Quentin , Pelletier Eric , Wessner Marc , Leconte Jade , Mangot Jean-François , Poulain Julie , Labadie Karine , Logares Ramiro , Sunagawa Shinichi , de Berardinis Véronique , Salanoubat Marcel , Dimier Céline , Kandels-Lewis Stefanie , Picheral Marc , Searson Sarah ,   Tara Oceans Coordinators, Acinas Silvia G. , Boss Emmanuel , Follows Michael , Gorsky Gabriel , Grimsley Nigel , Karp-Boss Lee , Krzic Uros , Not Fabrice , Ogata Hiroyuki , Raes Jeroen , Reynaud Emmanuel G. , Sardet Christian , Speich Sabrina , Stemmann Lars , Velayoudon Didier , Weissenbach Jean , Pesant Stephane , Poulton Nicole , Stepanauskas Ramunas , Bork Peer , Bowler Chris , Hingamp Pascal , Sullivan Matthew B. , Iudicone Daniele , Massana Ramon , Aury Jean-Marc , Henrissat Bernard , Karsenti Eric , Jaillon Olivier , Sieracki Mike , de Vargas Colomban , Wincker Patrick 
Published: January 2018

AbstractSingle-celled eukaryotes (protists) are critical players in global biogeochemical cycling of nutrients and energy in the oceans. While their roles as primary producers and grazers are well appreciated, other aspects of their life histories remain obscure due to challenges in culturing and sequencing their natural diversity. Here, we exploit single-cell genomics and metagenomics data from the circumglobal Tara Oceans expedition to analyze the genome content and apparent oceanic distribution of seven prevalent lineages of uncultured heterotrophic stramenopiles. Based on the available data, each sequenced genome or genotype appears to have a specific oceanic distribution, principally correlated with water temperature and depth. The genome content provides hypotheses for specialization in terms of cell motility, food spectra, and trophic stages, including the potential impact on their lifestyles of horizontal gene transfer from prokaryotes. Our results support the idea that prominent heterotrophic marine protists perform diverse functions in ocean ecology.
Re-analysis of public genetic data reveals a rare X-chromosomal variant associated with type 2 diabetes
Authors: Bonàs-Guarch Sílvia , Guindo-Martínez Marta , Miguel-Escalada Irene , Grarup Niels , Sebastian David , Rodriguez-Fos Elias , Sánchez Friman , Planas-Fèlix Mercè , Cortes-Sánchez Paula , González Santi , Timshel Pascal , Pers Tune H. , Morgan Claire C. , Moran Ignasi , Atla Goutham , González Juan R. , Puiggros Montserrat , Martí Jonathan , Andersson Ehm A. , Díaz Carlos , Badia Rosa M. , Udler Miriam , Leong Aaron , Kaur Varindepal , Flannick Jason , Jørgensen Torben , Linneberg Allan , Jørgensen Marit E. , Witte Daniel R. , Christensen Cramer , Brandslund Ivan , Appel Emil V. , Scott Robert A. , Luan Jian’an , Langenberg Claudia , Wareham Nicholas J. , Pedersen Oluf , Zorzano Antonio , Florez Jose C , Hansen Torben , Ferrer Jorge , Mercader Josep Maria , Torrents David 
Published: January 2018

AbstractThe reanalysis of existing GWAS data represents a powerful and cost-effective opportunity to gain insights into the genetics of complex diseases. By reanalyzing publicly available type 2 diabetes (T2D) genome-wide association studies (GWAS) data for 70,127 subjects, we identify seven novel associated regions, five driven by common variants (LYPLAL1, NEUROG3, CAMKK2, ABO, and GIP genes), one by a low-frequency (EHMT2), and one driven by a rare variant in chromosome Xq23, rs146662075, associated with a twofold increased risk for T2D in males. rs146662075 is located within an active enhancer associated with the expression of Angiotensin II Receptor type 2 gene (AGTR2), a modulator of insulin sensitivity, and exhibits allelic specific activity in muscle cells. Beyond providing insights into the genetics and pathophysiology of T2D, these results also underscore the value of reanalyzing publicly available data using novel genetic resources and analytical approaches.
Lysophosphatidylcholine acyltransferase 2-mediated lipid droplet production supports colorectal cancer chemoresistance
Authors: Cotte Alexia Karen , Aires Virginie , Fredon Maxime , Limagne Emeric , Derangère Valentin , Thibaudin Marion , Humblin Etienne , Scagliarini Alessandra , de Barros Jean-Paul Pais , Hillon Patrick , Ghiringhelli François , Delmas Dominique 
Published: January 2018

AbstractLipid droplet (LD) accumulation is a now well-recognised hallmark of cancer. However, the significance of LD accumulation in colorectal cancer (CRC) biology is incompletely understood under chemotherapeutic conditions. Since drug resistance is a major obstacle to treatment success, we sought to determine the contribution of LD accumulation to chemotherapy resistance in CRC. Here we show that LD content of CRC cells positively correlates with the expression of lysophosphatidylcholine acyltransferase 2 (LPCAT2), an LD-localised enzyme supporting phosphatidylcholine synthesis. We also demonstrate that LD accumulation drives cell-death resistance to 5-fluorouracil and oxaliplatin treatments both in vitro and in vivo. Mechanistically, LD accumulation impairs caspase cascade activation and ER stress responses. Notably, droplet accumulation is associated with a reduction in immunogenic cell death and CD8+ T cell infiltration in mouse tumour grafts and metastatic tumours of CRC patients. Collectively our findings highlight LPCAT2-mediated LD accumulation as a druggable mechanism to restore CRC cell sensitivity.
Ambient PM2.5 exposure and expected premature mortality to 2100 in India under climate change scenarios
Authors: Chowdhury Sourangsu , Dey Sagnik , Smith Kirk R. 
Published: January 2018

AbstractPremature mortality from current ambient fine particulate (PM2.5) exposure in India is large, but the trend under climate change is unclear. Here we estimate ambient PM2.5 exposure up to 2100 by applying the relative changes in PM2.5 from baseline period (2001–2005) derived from Coupled Model Inter-comparison Project 5 (CMIP5) models to the satellite-derived baseline PM2.5. We then project the mortality burden using socioeconomic and demographic projections in the Shared Socioeconomic Pathway (SSP) scenarios. Ambient PM2.5 exposure is expected to peak in 2030 under the RCP4.5 and in 2040 under the RCP8.5 scenario. Premature mortality burden is expected to be 2.4–4 and 28.5–38.8% higher under RCP8.5 scenario relative to the RCP4.5 scenario in 2031–2040 and 2091–2100, respectively. Improved health conditions due to economic growth are expected to compensate for the impact of changes in population and age distribution, leading to a reduction in per capita health burden from PM2.5 for all scenarios except the combination of RCP8.5 exposure and SSP3.
Fluid network dynamics in the prefrontal cortex during multiple strategy switching
Authors: Malagon-Vina Hugo , Ciocchi Stephane , Passecker Johannes , Dorffner Georg , Klausberger Thomas 
Published: January 2018

AbstractCoordinated shifts of neuronal activity in the prefrontal cortex are associated with strategy adaptations in behavioural tasks, when animals switch from following one rule to another. However, network dynamics related to multiple-rule changes are scarcely known. We show how firing rates of individual neurons in the prelimbic and cingulate cortex correlate with the performance of rats trained to change their navigation multiple times according to allocentric and egocentric strategies. The concerted population activity exhibits a stable firing during the performance of one rule but shifted to another neuronal firing state when a new rule is learnt. Interestingly, when the same rule is presented a second time within the same session, neuronal firing does not revert back to the original neuronal firing state, but a new activity-state is formed. Our data indicate that neuronal firing of prefrontal cortical neurons represents changes in strategy and task-performance rather than specific strategies or rules.
Single crystal elasticity of natural topaz at high-temperatures
Authors: Tennakoon Sumudu , Peng Ye , Mookherjee Mainak , Speziale Sergio , Manthilake Geeth , Besara Tiglet , Andreu Luis , Rivera Fernando 
Published: January 2018

AbstractTopaz is an aluminosilicate mineral phase stable in the hydrothermally altered pegmatitic rocks and also in subducted sedimentary lithologies. In nature, topaz often exhibits solid solution between fluorine and hydrous end members. We investigated elasticity of naturally occurring single crystal topaz (Al2SiO4F1.42(OH)0.58) using Resonant Ultrasound Spectroscopy. We also explored the temperature dependence of the full elastic constant tensor. We find that among the various minerals stable in the Al2O3-SiO2-H2O ternary system, topaz exhibits moderate elastic anisotropy. As a function of temperature, the sound velocity of topaz decreases with $$\frac{{\boldsymbol{d}}{{\boldsymbol{V}}}_{{\boldsymbol{P}}}}{{\boldsymbol{d}}{\boldsymbol{T}}}$$

d

V

P

d
T

and $$\frac{{\boldsymbol{d}}{{\boldsymbol{V}}}_{{\boldsymbol{S}}}}{{\boldsymbol{d}}{\boldsymbol{T}}}$$

d

V

S

d
T

being −3.10 and −2.30 × 10−4 km/s/K. The elasticity and sound velocity of topaz also vary as a function of OH and F content. The effect of composition ($${{\boldsymbol{x}}}_{{\boldsymbol{O}}{\boldsymbol{H}}}$$

x

O
H

) on the velocity is equally important as that of the effect of temperature. We also note that the Debye temperature ($${{\boldsymbol{\Theta }}}_{{\boldsymbol{D}}}$$

Θ

D

) of topaz at room temperature condition is 910 K and decreases at higher temperature. The Debye temperature shows positive correlation with density of the mineral phases in the Al2O3-SiO2-H2O ternary system.
Absolute spectroscopy near 7.8 μm with a comb-locked extended-cavity quantum-cascade-laser
Authors: Lamperti Marco , AlSaif Bidoor , Gatti Davide , Fermann Martin , Laporta Paolo , Farooq Aamir , Marangoni Marco 
Published: January 2018

AbstractWe report for the first time the frequency locking of an extended-cavity quantum-cascade-laser (EC-QCL) to a near-infrared frequency comb. The locked laser source is exploited to carry out molecular spectroscopy around 7.8 μm with a line-centre frequency combined uncertainty of ~63 kHz. The strength of the approach, in view of an accurate retrieval of line centre frequencies over a spectral range as large as 100 cm−1, is demonstrated on the P(40), P(18) and R(31) lines of the fundamental rovibrational band of N2O covering the centre and edges of the P and R branches. The spectrometer has the potential to be straightforwardly extended to other spectral ranges, till 12 μm, which is the current wavelength limit for commercial cw EC-QCLs.
Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin
Authors: Stirm Laura , Huypens Peter , Sass Steffen , Batra Richa , Fritsche Louise , Brucker Sara , Abele Harald , Hennige Anita M. , Theis Fabian , Beckers Johannes , Hrabě de Angelis Martin , Fritsche Andreas , Häring Hans-Ulrich , Staiger Harald 
Published: January 2018

AbstractThe number of pregnancies complicated by gestational diabetes (GDM) is increasing worldwide. To identify novel characteristics of GDM, we studied miRNA profiles of maternal and fetal whole blood cells (WBCs) from GDM and normal glucose tolerant (NGT) pregnant women matched for body mass index and maternal age. After adjustment for maternal weight gain and pregnancy week, we identified 29 mature micro-RNAs (miRNAs) up-regulated in GDM, one of which, i.e., miRNA-340, was validated by qPCR. mRNA and protein expression of PAIP1, a miRNA-340 target gene, was found down-regulated in GDM women, accordingly. In lymphocytes derived from the mothers’ blood and treated in vitro, insulin increased and glucose reduced miRNA-340 expression. In fetal cord blood samples, no associations of miRNA-340 with maternal GDM were observed. Our results provide evidence for insulin-induced epigenetic, i.e., miRNA-dependent, programming of maternal WBCs in GDM.
High geothermal heat flux in close proximity to the Northeast Greenland Ice Stream
Authors: Rysgaard Søren , Bendtsen Jørgen , Mortensen John , Sejr Mikael K. 
Published: January 2018

AbstractThe Greenland ice sheet (GIS) is losing mass at an increasing rate due to surface melt and flow acceleration in outlet glaciers. Currently, there is a large disagreement between observed and simulated ice flow, which may arise from inaccurate parameterization of basal motion, subglacial hydrology or geothermal heat sources. Recently it was suggested that there may be a hidden heat source beneath GIS caused by a higher than expected geothermal heat flux (GHF) from the Earth’s interior. Here we present the first direct measurements of GHF from beneath a deep fjord basin in Northeast Greenland. Temperature and salinity time series (2005–2015) in the deep stagnant basin water are used to quantify a GHF of 93 ± 21 mW m−2 which confirm previous indirect estimated values below GIS. A compilation of heat flux recordings from Greenland show the existence of geothermal heat sources beneath GIS and could explain high glacial ice speed areas such as the Northeast Greenland ice stream.
Tuning bandgap and surface wettability of NiFe2O4 driven by phase transition
Authors: Tong Sheng-Kai , Chi Po-Wei , Kung Shu-Hsiang , Wei Da-Hua 
Published: January 2018

AbstractStress variation induced bandgap tuning and surface wettability switching of spinel nickel ferrite (NiFe2O4, NFO) films were demonstrated and directly driven by phase transition via a post-annealing process. Firstly, the as-deposited NFO films showed hydrophilic surface with water contact angle (CA) value of 80 ± 1°. After post-annealing with designed temperatures ranged from 400 to 700 °C in air ambience for 1 hour, we observed that the crystal structure was clearly improved from amorphous-like/ nanocrystalline to polycrystalline with increasing post-annealing temperature and this phenomenon is attributed to the improved crystallinity combined with relaxation of internal stress. Moreover, super-hydrophilic surface (CA = 14 ± 1°) was occurred due to the remarkable grain structure transition. The surface wettability could be adjusted from hydrophilicity to super-hydrophilicity by controlling grain morphology of NFO films. Simultaneously, the saturation magnetization (Ms) values of NFO films at room temperature increased up to 273 emu/cm3 accompanied with transitions of the phase and grain structure. We also observed an exceptionally tunable bandgap of NFO in the range between 1.78 and 2.72 eV under phase transition driving. Meanwhile, our work demonstrates that direct grain morphology combined with the stress tuning can strongly modulate the optical, surface and magnetic characteristics in multifunctional NFO films.
Kinin-B1 Receptor Stimulation Promotes Invasion and is Involved in Cell-Cell Interaction of Co-Cultured Glioblastoma and Mesenchymal Stem Cells
Authors: Oliveira Mona N. , Pillat Micheli M. , Motaln Helena , Ulrich Henning , Lah Tamara T. 
Published: January 2018

AbstractGlioblastoma multiforme (GBM) represents the most lethal brain tumour, and these tumours have very limited treatment options. Mesenchymal stem cells (MSC) are considered as candidates for advanced cell therapies, due to their tropism towards GBM, possibly affecting their malignancy, thus also representing a potential therapeutic vector. Therefore, we aimed to compare the effects of bone-marrow-derived versus adipose-tissue-derived MSC (BM-/AT-MSC) on heterogeneous populations of tumour cells. This cells’ interplay was addressed by the in-vitro two-dimensional (monolayer) and three-dimensional (spheroid) co-culture models, using U87 and U373 GBM cell lines, expressing genotypically different mesenchymal transcriptome profiles. U87 cell low mesenchymal profile expressed high levels of kinin receptor 1 (B1R) and their invasion was greatly enhanced by the B1R agonist des-Arg9-bradykinin upon BM-MSC co-culturing in 3D co-cultures. This correlated to significantly higher cell-cell interactions in U87/BM-MSC mixed spheroids. This was not observed with the U373 cells and not in AT-MSC co-cultures. Altogether, these data support the on-going exploration of B1R as target for adjuvant approach in GBM therapy. Secondly, the results emphasize the need for further careful exploration of the selectivity regarding the origin of MSC as potential candidates for cell therapies, particular in cancer, where they may adversely affect heterogeneous tumour cell populations.
Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
Authors: Tóth Renáta , Cabral Vitor , Thuer Ernst , Bohner Flóra , Németh Tibor , Papp Csaba , Nimrichter Leonardo , Molnár Gergő , Vágvölgyi Csaba , Gabaldón Toni , Nosanchuk Joshua D. , Gácser Attila 
Published: January 2018

AbstractInvasive candidiasis is among the most life-threatening infections in patients in intensive care units. Although Candida albicans is the leading cause of candidaemia, the incidence of Candida parapsilosis infections is also rising, particularly among the neonates. Due to differences in their biology, these species employ different antifungal resistance and virulence mechanisms and also induce dissimilar immune responses. Previously, it has been suggested that core virulence effecting transcription regulators could be attractive ligands for future antifungal drugs. Although the virulence regulatory mechanisms of C. albicans are well studied, less is known about similar mechanisms in C. parapsilosis. In order to search for potential targets for future antifungal drugs against this species, we analyzed the fungal transcriptome during host-pathogen interaction using an in vitro infection model. Selected genes with high expression levels were further examined through their respective null mutant strains, under conditions that mimic the host environment or influence pathogenicity. As a result, we identified several mutants with relevant pathogenicity affecting phenotypes. During the study we highlight three potentially tractable signaling regulators that influence C. parapsilosis pathogenicity in distinct mechanisms. During infection, CPAR2_100540 is responsible for nutrient acquisition, CPAR2_200390 for cell wall assembly and morphology switching and CPAR2_303700 for fungal viability.
Radiographic Knee Osteoarthritis and Knee Pain: Cross-sectional study from Five Different Racial/Ethnic Populations
Authors: Wang Ke , Kim Hyun A. , Felson David T. , Xu Ling , Kim Dong H. , Nevitt Michael C. , Yoshimura Noriko , Kawaguchi Hiroshi , Lin Jianhao , Kang Xiaozheng , Zhang Yuqing 
Published: January 2018

AbstractThe weak correlation between pain and structural changes in knee osteoarthritis is widely reported. In a previous within-person, knee-matched case-control study among Caucasians, the severity of radiographic osteoarthritis (ROA) was strongly associated with both the presence of frequent knee pain and pain severity. We studied the association between ROA and knee pain in five racial/ethnic populations by using the same method. Subjects were selected from China; Japan; Korea and the United States. Among subjects with knees discordant for either frequent knee pain or pain severity, we examined the relationship between ROA and the presence of frequent knee pain using conditional logistic regression, and between ROA and pain severity using a stratified proportional odds model with an amalgamating conditional likelihood. In total, 252 urban Chinese, 221 rural Chinese, 297 Japanese, 122 Korean, 1,735 Caucasian, and 394 African-American patients were included. There was a strong dose-response relationship between the severity of ROA and the prevalence of frequent knee pain in all five racial/ethnic populations. Even mild ROA was significantly associated with frequent knee pain. In addition, ROA was also strongly associated with the severity of knee pain. These results show that structural pathology is associated with knee pain in different ethnic populations.