bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxic and Apoptotic-inducing Effect of Fraction Containing Brazilein from Caesalpinia sappan L. and Cisplatin on T47D Cell Lines
Authors: Tirtanirmala Prisnu , Novarina Annisa , Utomo Rohmad Yudi , Sugiyanto Raisatun Nisa , Jenie Riris Istighfari , Meiyanto Edy 
Published: January 2018

Anticancer activity of secang’s heartwood (Caesalpinia sappan L.) is based on its main compound: brazilin and brazilein. Brazilin, brazilein, and other compounds such as caesalpiniaphenol can affect proteins that have a role in apoptosis. In this study, we observed cytotoxic activity of fraction containing brazilein (FCB) alone or in combination with chemotherapeutic agent, cisplatin and the ability of the combination to induce apoptosis in T47D breast cancer cell lines. Cytotoxicity assay was determined using MTT assay, whereas the detection apoptosis induction was conducted using flow cytometry using Annexin-V and propidium iodide. FCB and cisplatin showed cytotoxic effect on T47D cells with IC50 value of 68 µg/mL and 16 µM, respectively. Combination of FCB and cisplatin result synergistic combination at the concentration ratio of 1/2 IC50 with CI value of 0.66. Its combination also able to induce apoptosis on T47D cell population 13% larger than the single treatment. Based on this study, we conclude that FCB is able to enhance the cytotoxic effects of cisplatin by inducing apoptosis.Keywords: Caesalpinia sappan L., cisplatin, apoptosis, breast cancer
Effect of Propolis Extract to Heal The Burns in New Zealand Rabbit
Authors: Hilaria Maria , Elisma Elisma , Uhe Adrianus L. 
Published: January 2018

Propolis contains arginine (amino acids), ferulic acid (a derivative compound cinnamic which promotes the formation of collagen), flavonoids (phenolic compounds), and other compounds that can prevent the growth of bacteria, virus, and fungi. The purpose of this study was to measure the most concentration of propolis extracts that effective to heal burns of New Zealand rabbit's. This was an experimental study with the post-test only control group design. As many as 15 male white rabbits were divided into 5 groups which treated by propolis extract with concentration of 50%, 75%, 100%, positive control by bioplacenton and negative control with propylene glycol. The macroscopic observation was done with detached necrosis tissue and the growth of collagen tissue as the parameter. Data were analyzed by using the one-way ANOVA and continued by the SNK test. The results of this study indicated that the propolis extracted have potency on healing burn wound with the significance of 0.00 (p≤0,05). In the concentration of 100%, extract of propolis has potency on healing burn wound that proportionates with bioplacenton with the significance value of 1.000 (p≥0,05). It is concluded that by increasing dose, the healing time of burn wound will be shortened.Keywords : Propolis, healing burns, rabbit
The Gastroprotective Activity of Ethanol Extract of Curcuma domestica Val. on Mice Induced Ethanol - HCl
Authors: Farikha Fitri Rahmania , Bachri Moch. Saiful 
Published: January 2018

Curcuma domestica Val. (CD) is one of plant used as traditional medicine in around the world. One of the benefit of CD is to treat peptic ulcers. The aim of preset study to prove that the ethanol extract of CD has gastroprotective activity. This study used 25 male mice were divided into 5 groups. Group I as a control group were given suspension of 0.5% Na-CMC, groups II, III, and IV were each given ethanol extract of CD suspense with 0.5% Na-CMC at a dose of 50 mg/kg; 100 mg/kg; 200 mg/kg respectively, and group V was given ranitidine 10 mg/kg. All groups were treated orally for 6 days, then fasted for 24 hours, on the seventh day all mice induced 3M HCl - ethanol 60% (1:1) 0.2 ml/25 gBW. One hour later, mice were sacrificed by cervical dislocation, then dissected and taken gastric. Then performed an ulcer scoring and the curative ratio is calculated later in the histopathologic test. Data were analyzed using Kolmogorov-Smirnov test and the Levene test and ANOVA followed by LSD test level of 95%. The results showed there was no significant difference between the control with dose 50 mg/kgBW (p>0.05) and showed significant difference between control with dose 100 mg/kgBW (p<0.05),dose of 200 mg/kgBW (p<0,05), and ranitidine 10 mg/kgBW (p<0.05). Curative ratio percentage dose of 100 mg/kgBW; 200 mg/kgBW, and ranitidine are 38.89%, 61.11% and 66.67 % respectively. The conclusion of this research, the ethanol extract of Curcuma domestica Val at the dose 100 mg/kgBW and 200 mg/kgBW showed have gastroprotective activity.Keywords: Gastroprotective, Curcuma domestica Val., ethanol-HCl, Gastric ulcer
The Effect of Marigold Leaves and Doxorubicin Toward Cell Cycle and Apoptosis of T47D Cells
Authors: Fauzia Anis , Wati Umi Any Tiyas , Rizkita Cahyaning Gita , Qori'ah Eka Wahyuni Nurul , Arifin Ibrahim 
Published: January 2018

Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of side effect using of doxorubicin such as cardiomyopathy and congestive heart failure which can lead to death. One of the approach to overcome overloaded use of doxorubicin is the combined use with a chemopreventive agent (cochemotherapy), including the leaf extract of marigolds (Cosmos caudatus Kunth.). This research aims to reviewing the effect of the methanol extract of leaves of marigolds on the cytotoxic activity of doxorubicin in modulating cell cycle and apoptosis of breast cancer cells T47D. Determination of the cytotoxic activity of methanol extract of leaves of marigolds and doxorubicin as well as a combination of both conducted by MTT assay. modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of leaves of marigolds produce use values of IC50 504,840 μg/ml, whereas IC50 values doxorubicin is 141,055 nM. The synergistic effect was shown a combination of methanol extract leaves marigolds and doxorubicin at concentrations of 84,17 μg/ml -23,5 nM; 84,17 μg/ml -47 nM; 126,25 μg/ml -23,5 nM; 252,5 μg/ml -35,25 nM and 252,5 μg/ml -71 nM with a combination index value (CI) consecutively of 0,5; 0,6; 0,6; 0,6 and 0,6. Observations modulation of cell cycle and apoptosis induction combination of methanol extract leaves marigolds and doxorubicin at concentrations of 84,17 μg/ml -23,5 nM, said that a combination of the methanol extract of leaves of marigolds and doxorubicin to inhibit the proliferation of cells in G2 / M phase and able to induce apoptosis of breast cancer cells T47D.Keywords: methanol extract of leaves of marigolds, doxorubicin, flowcytometry, cell cycle, apoptosis.
Anticancer Effect of a Typhonium flagelliforme L. in Raji Cells Through Telomerase Expression
Authors: Purwaningsih Endang , Suciati Yulia , Widayanti Etty 
Published: January 2018

Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice (Typhonium flagelliforme) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts (Typhonium flagelliforme) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract (Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract (Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase.Keywords: telomerase, IHC, Typhonium flagelliforme
Ethyl Acetate Fraction of Caesalpinia sappan L. Enhances Cisplatin’s Cytotoxicity on HeLa Cells via G1 and S Arrest through p53 Expression
Authors: Husnaa Ulfatul , Laksmiani Ni Putu Linda , Susidarti Ratna Asmah , Meiyanto Edy 
Published: January 2018

Cisplatin (cisp) is the first line chemotherapeutic agent for several cancer diseases which can cause significant side effects and cellular resistance. Combination-chemotherapy treatment (co-chemotherapy) was reported to be able to reduce cisp effects. Therefore, this study was carried out to investigate the cytotoxic activity of ethyl acetate fraction of C. sappan (EFC) in combination with cisp by observing apoptosis induction and cell cycle profile. Cytotoxic activity was evaluated by MTT assay. Cell cycle and apoptosis analysis were performed using flow cytometry and p53 expression was analyzed using immunocytochemistry. EFC performed cytotoxic effect on HeLa cells by showing morphological changes such as cell shrinkage, rounding and decreasing of cells viability in concentration dependent manner, giving IC50 value of 65 μg/mL. Combination of EFC and cisp in low concentration decreased cell viability into 36.86%. Further assay indicated that this combination caused redistribution of cell cycle arrest in G1 and S phases through p53 stabilization in nucleus. However, that mechanism was not followed by apoptosis. These results provide evidence to support EFC development as the enhancer of cisp effect, by improving its cytotoxicity on HeLa cells. EFC increases HeLa cells sensitivity to cisp through G1 and S cells’ arrest depending on p53 expression. Key words: co-chemotherapy, EFC, cervix cancer HeLa cells, p53, G1 and S arrest.
Doxorubicin Induces Lamellipodia Formation and Cell Migration
Authors: Amalina Nur , Nurhayati Ika Putri , Meiyanto Edy 
Published: January 2018

Breast cancer is the main cause of cancer death among women, especially breast cancer metastasis. Metastasis process begins with the ability of cell cancer invasion. Doxorubicin, a antracycline chemotheraphy, is known to induce TGFβ1, thus promote invasion. The aim of this study is to optimize doxorubicin doses to induce lamellipodia formation in 4T1 and MCF-7/HER2 cells. Lamellipodia formation was observed by morphological changes using microscope inverted. The effect of doxorubicin on cell viability was analyzed using MTT assay. Rac1 expression after doxorubicin exposure was determined by western blotting. Lamellipodia formation was observed by morphological change of the cell at the dose 10, 25, 50 and 100 nM. Doxorubicin at the dose of 10 nM could induced lamellipodia formation without affect cell viability in both 4T1 and MCF-7/HER2 cells. Doxorubicin induced cell cycle arrest at G2/M phase at all doses. Doxorubicin 10 nM also decrease Rac1 expression compared to control.Key words: Doxorubicin, lamellipodia, Rac1, migration.
The need for multiple types of information to inform climate change assessment
Authors: Toman Michael 
Published: January 2018

Abstract:Many critics of economic analysis of climate change impacts and response options claim that information is needed on ecosystem characteristics as well as on economic values to fully inform decisions about how climate change affects human well-being. Information on the irreversibility of impacts also is important, critics argue, because it relates to how society evaluates implications for intergenerational equity. In addition, because climate change is subject to a large degree of Knightian uncertainty, it is useful to understand both the information available for assessing climate change risks, and how individuals themselves perceive and evaluate risks. The paper discusses rationales for using these types of information as important complements to benefit-cost analysis for evaluating climate change risks and responses. Ideally such information could be available in a “dashboard” for decision makers assessing social and economic impacts, although limits on currently available information are a significant barrier to using that approach.
Tres líneas de comunes y democracia / Three common lines and democracy
Authors: Boza Santiago Celia 
Published: January 2018

Con la necesidad de poner fin a la comercialización de datos y dar lugar a la aparición de nuevas formas de gestión descentralizadas, aparece la línea de comunes que abogan por una ciudadanía más participativa. Este artículo se propone sistematizar y diferenciar tres líneas de comunes interrelacionadas, consideradas como motor del paso a una mayor transparencia para lo público, permitiendo la democracia directa. Finalmente, se proponen distintas alternativas de clasificación y gestión de los bienes comunes y redes de comunicación ciudadana que facilitan su comprensión en el contexto actual.
HÁBITOS MATERNOS RELACIONADOS À AMAMENTAÇÃO
Authors: Bezerra Jallyne Colares , Oliveira Rhaiany Kelly Lopes , Oliveira Brena Shellem Bessa , Sousa Suelen Alves , Melo Francisca Mayra Sousa , Joventino Emanuella Silva 
Published: January 2018

<p>Objetivo: caracterizar os hábitos relacionados ao aleitamento materno de crianças menores de cinco anos. Método: estudo descritivo, transversal, quantitativo. Foram entrevistadas 385 mães de crianças com idade inferior a cinco anos, em oito Unidades de Atenção Primária à Saúde do interior do Ceará, Brasil, no período de agosto a outubro de 2015.<br />Realizaram-se análises estatísticas descritivas. Resultados: constatou-se que 96,6% das mulheres amamentaram seus filhos, contudo, dessas, 40,7% amamentaram por um período inferior a seis meses. Das mães entrevistadas, 42,1% referiram não recorrer ao uso de chupeta para acalentar seus filhos, no entanto, 61,7% utilizaram mamadeira para a<br />alimentação da criança. Conclusão: constatou-se que um considerável número de mães desmamou precocemente seus filhos e, em consequência disso, elas introduziram novos alimentos antes do sexto mês de vida da criança.</p><p><br />Descritores: Aleitamento materno. Saúde da criança. Enfermagem.</p>
ANÁLISE DE CONFORMIDADE E NÃO CONFORMIDADE DE UNIDADES DE SAÚDE DA FAMÍLIA
Authors: Souza Mariluce Karla Bomfim de , Barreto Leidiane Andrade , Silva Elaine Andrade Leal 
Published: January 2018

<p>Objetivo: identificar e discutir conformidades e não conformidades de unidades de saúde da família, de dois municípios do estado da Bahia, Brasil. Método: estudo descritivo e de avaliação normativa realizado em 23 unidades de saúde de um município de grande e outro de médio porte, no Nordeste brasileiro, no ano de 2014. Utilizou-se um roteiro de check-list elaborado a partir de manual ministerial sobre a estrutura física de unidades, sendo considerada a regulamentação técnica da Agência Nacional de Vigilância Sanitária e o programa EpiData para registro e arquivamento dos dados. Resultados: condições estruturais adaptadas e adequações de imóveis para o funcionamento de estabelecimentos de saúde podem interferir sobre a qualidade da oferta dos serviços. Conclusão: constatou-se a necessidade de estruturação e de priorização da Atenção Primária à Saúde, a fim de não comprometer a mudança do modelo assistencial.</p><p><br />Descritores: Avaliação em saúde. Estrutura dos serviços. Atenção primária à saúde.</p>
PLANO DE PARTO COMO ESTRATÉGIA DE EMPODERAMENTO FEMININO
Authors: Mouta Ricardo José Oliveira , Silva Tania Maria De Almeida , De Melo Paula Titara Silva , Lopes Natália De Souza , Moreira Vanessa Dos Anjos 
Published: January 2018

<p>Objetivo: analisar como o plano de parto propiciou o empoderamento feminino durante o trabalho de parto e parto. Método: estudo exploratório qualitativo. Os dados foram coletados mediante a entrevista semiestruturada, entre maio e junho de 2016. O tratamento analítico empregado foi a análise de conteúdo. Resultados: a partir da análise dos dados emergiram três categorias: Plano de parto – um direito da mulher até então desconhecido; Plano de parto – uma tecnologia integrada à assistência do enfermeiro obstétrico; Plano de parto – uma tecnologia a favor do empoderamento feminino no parto. Com esse estudo, percebemos que existe um desconhecimento acerca do plano de parto,<br />independentemente da idade, escolaridade ou número de gestações das entrevistadas. Conclusão: destacou-se a importância do enfermeiro obstétrico atuando em partos e utilizando o plano de parto como uma tecnologia não invasiva. A construção do plano de parto durante o pré-natal contribui para o desenvolvimento favorável do trabalho de parto.</p><p><br />Descritores: Enfermagem obstétrica. Parto humanizado. Poder. Mulher.</p>
Development of a Geomechanical Model for Drilling Deviated Wells through the Zubair Formation in Southern Iraq
Authors: Abbas Ahmed K. , Al-Asadi Yousif M. , Alsaba Mortadha , Flori Ralph E. , Alhussainy Shaban 
Published: January 2018

Abstract
The Zubair Formation is the most prolific reservoir in Iraq, which is comprised of sandstones interbedded with shale sequences. Drilling deviated boreholes in this formation has always been a challenge due to the weak nature of the shale sequence. Historically, over 90% of wellbore problems in the Zubair Formation are due to wellbore instability. These wellbore instability problems, such as hole collapse, tight hole, stuck pipe, and sidetracks results in increasing the nonproductive time (NPT) and well costs. The present study aims to construct a calibrated post-drill 1-D mechanical earth model (MEM) that can be used to conduct a comprehensive geomechanical analysis of the Zubair Formation.
A practical methodology was detailed for (1) constructing the 1-D MEM by using data from several open hole logs and (2) calibrating such a model using all the available data (e.g., observations of wellbore failures, measurements of well testing, laboratory test results of rock mechanical properties, etc.). Three failure criteria (i.e., Mohr-Coulomb, Mogi-Coulomb, and Modified Lade) were used to perform a root cause analysis of borehole breakouts and to address the minimum required mud weight to minimize wellbore instability-related issues for different well trajectories.
The wellbore failure analysis showed that the mud weight, that was used previously, is insufficient to support the rock on the wellbore wall, and it is not appropriately adjusted according to the variation in the wellbore azimuth and inclination. In addition, the heterogeneity of the Zubair Formation and the inappropriate drilling practice have magnified the severity of the drilling problems. The predictions resulting from this study can be used as cost-effective tools when planning for future neighboring deviated wells to create better drilling efficiency by reducing the nonproductive time and well costs.
Novel Modified Rectorite Provides Reliable Rheology and Suspendability for Biodiesel Based Drilling Fluid
Authors: Li Wai , Liu Jishan , Zhao Xionghu , Zhang Jian , Jiang Jiwei , He Tao , Liu Liu , Shen Peiyuan , Zhang Min 
Published: January 2018

Abstract
The use of organophilic clays as additives for providing proper rheological and filtration properties in non-aqueous drilling fluids (NADFs) has long been a topic of study. Currently, most of these clays are based on the modification of bentonite with quaternary ammonium salts. As new NADF systems emerging, novel clay-modification technologies are needed urgently to obtain more effective organophilic clays for specific drilling fluids. This paper introduced a modified rectorite designed for biodiesel-based drilling fluid (BBDF). Through a suspension production technique, rectorite, a clay mineral formed by dioctahedral mica layer and dioctahedral smectite layer with a 1:1 type of layer structure and a peculiar banded structure, was modified by non-ionic surfactants. The swell behavior of the modified rectorite in invert biodiesel emulsion was investigated. Effects of this novel organophilic clay on the electrical stability, rheological parameters and suspendability of biodiesel emulsion were also tested. The results showed that some non-ionic surfactants can be intercalated into the structure of rectorite, altering the wetting characteristic of the clay surface. Compared to traditional organophilic bentonite, modified rectorite has better swell performance in biodiesel emulsion. The abilities of modified rectorite to improve yield point (YP) and low end rheology (e.g., 6 rpm reading) of biodiesel emulsion are also significantly stronger than those of traditional organophilic bentonite, suggesting a higher efficiency of adjusting rheology and suspendability. Combined with appropriate rheological modifiers (RMs), the modified rectorite can provide formulated BBDF a great suspendability, which is evidenced by static barite-sag test. Electrical stability of biodiesel emulsion was also enhanced with the addition of the modified rectorite due to the forming of Pickering emulsion. This novel modified clay is a reliable guarantee of steady properties of BBDF. The origin of its good performance can be attributed to the selection of suitable surfactants and the rational preparation technology. Rectorite is widespread all around the world and there are some rectorite mining areas in various countries although the degree of utilization for this clay is still low at present. In view of the relatively lower price of rectorite compared to bentonite, modified rectorite can be considered as a high-performance, cost-effective viscosifier for BBDF and perhaps, other NADFs.
Retrieval of Intelligent Completions Challenges and Solution
Authors: Kamal Syed Muhammed , Teran Juan M. , M. Adamawi Marwan , Qazi Muhammed Qasim 
Published: January 2018

Abstract
De-completing wells — due to downhole problems related to tubing-casing annulus communication, casing leaks or prior to executing re-entry sidetracks for multilateral wells — is adaily activity in the oilfield business. Conventional completions comprised of downhole production packers and tubing are normally retrieved with no major issues. But this is not the case for intelligent completions, as they include a numberof downhole components that makes its retrieval more challenging, and there was no set procedure or provision in place to retrieve them, in case of failure or encountered downhole problems during the production life of the well.
Since intelligent completion deployments are increasing, the number of intelligent wells to be de-completed will also increase.
This paper presents the case history and experience gained during the de-completion of intelligent completions of different designs; highlights the challenges and difficulties in recovering of multiple packers and flow control valves; and the strategy to achieve full access to the wellbore, to re-establish production and recover assets. This paper will also summarize lessons to achieve successful de-completion of an intelligent well.