bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
Authors: Antov Georgi G. , Gospodinova Zlatina I. , Novakovic Miroslav , Tesevic Vele , Krasteva Natalia A. , Pavlov Danail V. , Valcheva-Kuzmanova Stefka V. 
Published: September 2024

Abstract
The aim of the present work was to investigate some of the molecular mechanisms and targets of the anticancer action of the bioflavonoid fustin isolated from the heartwood of Cotinus coggygria Scop. in the triple-negative breast cancer cell line MDA-MB-231. For this purpose, we applied fluorescence microscopy analysis to evaluate apoptosis, necrosis, and mitochondrial integrity, wound healing assay to study fustin antimigratory potential and quantitative reverse transcription-polymerase chain reaction to analyze the expression of genes associated with cell cycle control, programmed cell death, metastasis, and epigenetic alterations. A complex network-based bioinformatic analysis was also employed for protein–protein network construction, hub genes identification, and functional enrichment. The results revealed a significant induction of early and late apoptotic and necrotic events, a slight alteration of the mitochondria-related fluorescence, and marked antimotility effect after fustin treatment. Of 34 analyzed genes, seven fustin targets were identified, of which CDKN1A, ATM, and MYC were significantly enriched in pathways such as cell cycle, intrinsic apoptotic signaling pathway in response to DNA damage and generic transcription pathway. Our findings outline some molecular mechanisms of the anticancer action of fustin pointing it out as a potential oncotherapeutic agent and provide directions for future in vivo research.
Melatonin attenuates hepatic oxidative stress by regulating the P62/LC3 autophagy pathway in PCOS
Authors: Zhang Junhui , Zhang Hongyan , Guo Bao , Yang Jun , Yu Renxiang , Chen Wenxiu , Zhai Muxin , Cao Yuhan , Liu Yajing , Hong Qiang , Xie Fenfen 
Published: September 2024

Graphical abstract

The potential protective mechanisms of MT on hepatic OS in PCOS. MT could lead to a decrease in autophagy, presenting a decrease of LC3 and an increase of P62 in the PCOS liver. Increased P62 could reduce Nrf2 ubiquitination and increase its nuclear translocation and activation through direct interaction with Keap1. Thus, MT ultimately activated the Keap1-Nrf2 pathway, promoting nuclear Nrf2 expression to reduce hepatic OS.

Abstract
The elevated level of hepatic oxidative stress (OS) in polycystic ovary syndrome (PCOS) is one of the important causes of liver abnormalities. Therefore, decreasing the level of hepatic OS in PCOS is beneficial in reducing the risk of PCOS-related liver diseases. Melatonin (MT) is recognized as a potent antioxidant. Nevertheless, the efficacy of MT in alleviating hepatic OS associated with PCOS is yet to be established, and the precise mechanisms through which MT exerts its antioxidant effects remain to be fully elucidated. The aim of this study was to explore the potential mechanisms by which MT reduces hepatic OS in PCOS. First, we detected elevated OS levels in PCOS samples. Subsequently, with MT pretreatment, we discovered that MT could significantly diminish the levels of OS, liver triglycerides, total cholesterol, alanine aminotransferase, and aspartate aminotransferase, while concurrently ameliorating mitochondrial structural damage in the PCOS liver. Furthermore, we identified elevated autophagy levels in the liver of PCOS rats and an inhibition of the Keap1–Nrf2 pathway. Through MT pretreatment, the expression of LC3 was significantly decreased, while the Keap1–Nrf2 pathway was activated. Our study showed that MT could affect the Nrf2 pathway dependent on the P62/LC3 autophagy pathway, thereby attenuating hepatic OS in PCOS. These findings offer novel insights and research avenues for the study of PCOS-related liver diseases.
CLEAVAGE OF SOLID FILMS OF SUBMICRON THICKNESS AS A METHOD FOR PREPARING CROSS SECTIONS OF HETEROSTRUCTURES FOR HIGH-RESOLUTION TRANSMISSION MICROSCOPY
Authors: ВОРОБЬЁВ А.Б. , ГУТАКОВСКИЙ А.К. , ПРИНЦ В.Я. 
Published: September 2024

Подробно описан метод препарирования, позволяющий получить бездефектные поперечные срезы гетероструктур для просвечивающей электронной микроскопии и обеспечивающий достижение атомного разрешения на большой площади. Метод проиллюстрирован примерами изучения гетерограниц сложной формы, селективного окисления и селективного травления сверхрешёток.
A preparation method is described in detail, which allows one to obtain defect-free cross sections of heterostructures for transmission electron microscopy and ensures the achievement of atomic resolution over a large area. The method is illustrated with examples of studying heterointerfaces of complex shape, selective oxidation, and selective etching of superlattices.
MOLECULAR BEAM EPITAXY OF GERMANIUM ON SI (001) FOR PHOTODETECTORS IN THE SPECTRAL RANGE FROM 1.3 TO 1.55 ΜM
Authors: ФРИЦЛЕР К.Б. , ДЕРЯБИН А.С. , ЛОШКАРЕВ И.Д. , НИКИФОРОВ А.И. , ЧИСТОХИН И.Б. , КОЛЕСНИКОВ А.В. , ВАСИЛЕНКО А.П. , ПЧЕЛЯКОВ О.П. , СОКОЛОВ Л.В. , ПЕВЧИХ К.Э. , СВЕТИКОВ В.В. , ГУТАКОВСКИЙ А.К. 
Published: September 2024

Методом молекулярно-лучевой эпитаксии сформированы фоточувствительные слои германия на кремниевой подложке. Исследованы структурные, оптические и фотоэлектрические характеристики плёнок. Полученные данные подтверждают возможность использования изготовленных гетероструктур Ge на Si для создания фотодетекторов спектрального диапазона 1,3-1,55 мкм.
Photosensitive germanium layers on a silicon substrate have been formed by molecular beam epitaxy. The structural, optical and photovoltaic characteristics of the films have been investigated. The data obtained confirm the possibility of utilizing the developed Ge-on-Si heterostructures for the fabrication of photodetectors in the spectral range of 1.3-1.55 μm.
STRESS CONCENTRATION IN WELLBORE ZONES AT UNDERGROUND GAS STORAGES
Authors: СВАЛОВ А.М. 
Published: September 2024

Исследованы особенности формирования зон концентрации напряжений в призабойных зонах скважин при эксплуатации подземных хранилищ газа. Численным моделированием установлено, что на начальной стадии процесса закачки газа под высоким давлением в подземных хранилищах газа вблизи кровли продуктивного пласта в породе и цементной оболочке скважины развиваются разрушающие напряжения сдвига, сопоставимые с давлением закачки, что может приводить к потере герметичности затрубного пространства скважин. Для предотвращения потерь газа предложено проводить расширение ствола скважины в кровле продуктивного пласта и устанавливать в соответствующем месте колонны обсадных труб пружинный центратор с номинальным диаметром, превышающим диаметр ствола, что остановит распространение зоны разрушения цементного камня. Для снижения интенсивности разрушающих напряжений в породе угловые зоны при расширении ствола скважины в интервале продуктивного пласта целесообразно делать закругленными.
The author studies stress concentration in wellbore zones during operation of underground gas storages. Numerical modeling yields that at the early stage of high-pressure injection of gas in underground gas storages, near the roof of a productive stratum, in rocks and in the cement lining, destructive shearing stresses arise, comparable with the injection pressure, which can induce the loss of tightness in the annular space. For preventing the loss of gas, it is proposed to ream the wells in the roof of a productive stratum and to install a spring centralizer at a certain place in the casing string. The nominal diameter of the centralizer should exceed the wellbore diameter. This can stop propagation of cement destruction. For decreasing the destructive stress intensity, it is advisable to round off corner zones while reaming within the interval of a productive strata.
INFLUENCE OF NATURAL ADDITIVE ON SLURRYABILITY AND FLOWABILITY OF IRON ORE
Authors: ГУПТА Ч. , КУМАР С. 
Published: September 2024

Приведено детальное описание и стабилизация концентрированной суспензии железной руды (размер частиц ≤ 75 мкм) с помощью различных лабораторных испытаний. Рассмотрены реологические характеристики железной руды в диапазоне весовой концентрации 60 - 80 % при наличии и отсутствии экстракта Sapindus mukorossi (мыльного дерева) в качестве диспергатора. Устойчивость суспензии железной руды при наличии сапонина определяется реологическими свойствами, концентрацией диспергатора и механизмом стабилизации. Выполнен регрессионный анализ экспериментальных реологических данных при различной скорости сдвига; полученные результаты хорошо согласуются с моделью Гершеля - Балкли. Критическая мицеллярная концентрация водного экстракта диспергатора составила 0.018 г/см3. Наличие сапонина значительно улучшило смешиваемость и устойчивость суспензии железной руды. Анализ напорных потерь и удельного энергопотребления доказывает экономическую целесообразность использования поверхностно-активных веществ для транспортировки рудной пульпы по трубопроводам.
The present article describes the extensive characterization and stabilization of concentrated iron ore suspension having size ≤ 75 µm by various bench-scale tests. The rheological characteristics of iron ore in the concentration range of 60-80% (by wt.) have been investigated with and without the addition of Sapindus Mmukorossi dispersant. The stability of iron ore suspension with saponin is established through rheological properties, dispersant concentration and stabilization mechanism. The nature of experimental rheological data at different shear rates is accomplished by regression analysis and found to be a good fit with Herschel-Bulkley model. The Critical Micellar Concentration of the aqueous extracted dispersant is 0.018 g/cc. The presence of Sapindus Mukorossi saponin greatly improved the slurryability and stability of iron ore suspension. The head loss and specific energy consumption analysis successfully evidence the economic relevance of the surfactants in transporting the slurry through the pipelines.
Trends in Enterobacterales Bloodstream Infections in Children
Authors: Gibbs Anna A. M. , Laupland Kevin B. , Edwards Felicity , Ling Weiping , Channon-Wells Samuel , Harley David , Falster Kathleen , Paterson David L. , Harris Patrick N. A. , Irwin Adam D. 
Published: September 2024

OBJECTIVES
Enterobacterales bloodstream infections (E-BSI) cause a significant burden of disease in children and are associated with antimicrobial resistance. We assessed temporal changes in the population-based incidence of E-BSI in children in Queensland, Australia.

METHODS
We conducted a cohort study of incidents of E-BSI occurring in children in Queensland between 2000 and 2019, with a total population of 19.7 million child years. Infections were linked to clinical outcomes in hospital admissions and vital statistics databases. We estimated age- and sex-standardized E-BSI incidence rates over time. Secondary outcomes included the proportion of extended-spectrum β-lactamase phenotypes per year, hospital length of stay, and mortality.

RESULTS
We identified 1980 E-BSI in 1795 children. The overall age- and sex-standardized incidence rate was 9.9 cases per 100 000 child years, which increased from 7.3 to 12.9 over the period studied, an increase of 3.9% (95% confidence interval: 3.1–4.7) per year. There were 3.6 cases of E. coli bloodstream infection per 100 000 child years, increasing annually by 4.7% (3.5–5.9). The Salmonella sp. bloodstream infection incidence was 3.0 cases per 100 000 child years, which increased from 2013 by 13.7% (3.8–24.3) per year. The proportion of extended-spectrum β-lactamase E. coli increased over time. Mortality and length of stay were higher among children with comorbidities than those without (4.0% vs 0.3%, and 14 vs 4 days, respectively, P &lt; .001).

CONCLUSIONS
The age- and sex-standardized incidence of E-BSI almost doubled in Queensland children over 2 decades, driven by increases in Salmonella sp. and E. coli. Increasing resistance of E. coli should prompt the inclusion of children in antimicrobial clinical trials.
Diagnosing the Level of Forming the Practical Component of Future Physical Education and Sports Specialists’ Readiness for Educational Activities to achieve Sustainable Development
Authors: Tsyhura Halyna , Harkusha Serhii 
Published: September 2024

Background. Ukraine’s support for sustainable development strategies and European integration processes requires the training of specialists engaged in sustainable development educational activities.
Objectives. The study aimed to show the impact of the experimental methodological system on the forming the practical component of physical education for future physical education and sports specialists’ readiness for educational activities contributing to sustainable development.
Materials and methods. The study involved 390 students from the faculties of physical education of the T. H. Shevchenko National University “Chernihiv Colehium”, the National University of Life and Environmental Sciences of Ukraine, Sumy State University, and Sumy State Pedagogical University named after A. S. Makarenko, future specialists in physical culture and sports. The students were informed about the features of the study and voluntarily participated in the pedagogical experiment. The participants of the study are represented by both sexes of different courses, from which the control and experimental groups were formed. The research methods used include the study and analysis of scientific and scientific-methodical literature, pedagogical experiments, surveys, testing, analyses, synthesis and generalisation of information, as well as methods of mathematical statistics.
Results. At the beginning of the study, 93.33 % of students in the control group and 94.44 % of students in the experimental group had a low level of the practical component of readiness for educational activities contributing to sustainable development. The final stage of the study revealed a significant improvement in the studied indicator within the experimental group: 43.33 % of students demonstrated a low level of the practical component of the studied readiness, 16.67 % had a satisfactory level, 31.11 % showed an average level, and 8.89 % had a high level. The corresponding changes were observed in all defined indicators: activities for sustainable development in physical education classes or sectional sports lessons; activities for sustainable development during extracurricular work of a physical education teacher or outside the sectional work of a sports coach with a population of different age groups; and activities for sustainable development in managerial positions. In the control group, at the end of the experiment, no significant changes were found in the the practical component levels of future physical education and sports specialists’ readiness to participate in educational activities for sustainable development.
Conclusions. The findings of the pedagogical experiment confirmed the positive impact of the developed methodological system on the forming the practical component of future physical culture and sports specialists’ readiness to participate in educational activities for sustainable development.
Agreement between AST from minute colony (8-10h growth) and mature colony (16-18h/overnight incubation)
Authors: Sastry Apurba S , Padhy Sonali , Priyadarshi Ketan , Dhandapani Sarumathi , Punnen Stessy Ann 
Published: September 2024

Sepsis is a medical emergency where a successful patient outcome depends on early and appropriate antibiotic treatment. This study was conducted to evaluate agreement between antimicrobial susceptibility testing (AST) from minute colonies that can reduce the AST-TAT by as much as 8-10h as compared to the CLSI recommended protocol of performing AST from an overnight (16-18h) growth of mature colony.In the present study, mDD results from minute colony (8-10h growth) were compared to the rDD results mature colony (16-18h/overnight incubation) CA and various types of errors were evaluated.237 pathogens and 1597 organism-antibiotic combinations were evaluated, there was a CA of 93.30% which was extremely satisfactory and categorical disagreement was found only in 4.56% of organism-antibiotic combinations, which were mainly mE (4.56%) with nil VME (0%) and ME (0%).We have found that minute colony (8-10h) AST is in agreement with reference mature colony (16-18h) AST, shortening TAT by (8-10h) earlier than the conventional reference method which is very helpful in treatment of sepsis patients.
Reflexiones sobre el uso de artificios y el conocimiento especializado del contenido matemático de docentes del nivel primaria
Authors: Dávila Vargas Kelly Jennifer , Garay Ramírez Sthefani Elena 
Published: September 2024

Este ensayo plantea que, cuando un docente de primaria posee una comprensión instrumental del contenido matemático, recurre a artificios para enseñar; es decir, utiliza procedimientos mecánicos para resolver problemas u operaciones matemáticas, simplificando los conceptos involucrados en la resolución. Sobre la base del marco del conocimiento matemático para la enseñanza propuesto por Ball et al. (2008) y el conocimiento de los temas (KoT) propuesto por Carrillo-Yañez et al. (2018), se describen los dominios de conocimientos implicados en la enseñanza de las matemáticas, en particular el conocimiento especializado del contenido matemático. Este no solo permite resolver un problema o realizar una operación correctamente, sino que implica comprender a profundidad las nociones matemáticas que subyacen y las conexiones entre estas. A través del análisis de algunos artificios, las autoras buscan promover la reflexión sobre la importancia del conocimiento especializado del contenido matemático en la formación docente.
Estrategias didácticas en la asesoría de tesis para desarrollar competencias investigativas: perspectivas de docentes y estudiantes
Authors: Tapullima-Mori Calixto , Montalvo Apolin Danitza Elfi , Bobadilla Bautista Sergio Daniel 
Published: September 2024

Introducción: La asesoría de tesis es un componente esencial en la formación académica. Durante este proceso los estudiantes se embarcan en la tarea de investigar y contribuir al conocimiento en su campo de estudio. Pero para que esto resulte, es necesario que cuenten con competencias específicas sobre la investigación y herramientas que contribuyan en su desarrollo. Objetivo: El objetivo fue analizar el uso de las estrategias didácticas de los docentes en el proceso de asesoría de tesis para desarrollar competencias investigativas en los estudiantes de últimos ciclos de educación superior. Método: Se siguió un diseño cualitativo fenomenológico exploratorio y se aplicaron entrevistas semiestructuradas a tres docentes expertos en investigación y a tres estudiantes de la Facultad de Psicología, pertenecientes a una universidad privada en la ciudad de Tarapoto-Perú, y que fueron seleccionados mediante un muestreo intencional por conveniencia. Resultados: Se comprendió que (a) las estrategias didácticas que se aplican en la enseñanza aprendizaje para el logro de los objetivos académicos son el trabajo colaborativo, el estudio de casos, el uso de recursos audiovisuales, el trabajo en equipo e incluyen, la asesoría y el rol docente; (b) se definen a las competencias investigativas como un conjunto de habilidades, capacidades y actitudes necesarias para realizar una investigación y proyectos de tesis; (c) además, es necesario saber formular problemas, hipotetizar, dominar Software estadísticos y argumentar los resultados eficientemente; y (d) es importante enfatizar la capacidad para el análisis de la coherencia metodológica y el uso de gestores bibliográficos. Discusión: Las ..
PROTAGONISMO E PIONEIRISMO NA CONSTITUIÇÃO DO HEROÍSMO FEMININO SURDO
Authors: Monteiro Rangel Gisele Maciel 
Published: September 2024

Com o objetivo de apresentar a constituição do heroísmo feminino surdo por meio da identificação de nomes de mulheres surdas consideradas pioneiras em suas áreas de atuação, a presente pesquisa busca valorizar as histórias da comunidade surda, reconhecer o protagonismo feminino em diferentes espaços sociais e políticos, e ampliar a lista de heroínas surdas. Para isso, o presente trabalho inscreve-se, teoricamente, na articulação entre o campo dos Estudos Culturais e Estudos Surdos. A produção dos dados deu-se por meio da metodologia de pesquisa em mídias sociais, com uma postagem na rede social Instagram, na qual a autora recebeu 184 comentários que mencionavam 34 nomes de mulheres surdas consideradas heroínas. Essas mulheres são referenciadas pelo pioneirismo em suas áreas de atuação e pela representatividade na comunidade surda. Considerando as interações na referida rede, é possível concluir que a presença e o protagonismo das mulheres surdas rompem com estereótipos e contribuem para o empoderamento das jovens surdas, marcando um deslocamento epistemológico nas últimas décadas. Assim, o pioneirismo e protagonismo feminino constituem elementos essenciais na constituição do heroísmo feminino surdo e abrem caminho para outros modos de ser surdo na contemporaneidade.
ANÁLISE COMPARATIVA ENTRE OS PAÍSES SULAMERICANOS: ATENDIMENTO ÀS CIDADÃS SURDAS
Authors: Krause Keli 
Published: September 2024

O presente trabalho faz parte da investigação da tese de doutorado, propondo uma análise comparativa entre os países sul-americanos (Brasil, Argentina, Paraguai, Uruguai e Venezuela), sob o viés da política cultural da comunidade surda. Teve como objetivo analisar o atendimento às mulheres surdas no âmbito da saúde e na esfera policial. Nesse trabalho, foram utilizados os pensamentos do filósofo e sociólogo alemão Axel Honneth (teoria de reconhecimento), e da filósofa norte-americana Nancy Fraser para discussão sobre reivindicação política e social como prática da organização cultural o que inclui também a questão da comunidade surda, especialmente o movimento das mulheres surdas nas esferas de ações institucionalizadas. Foram utilizados quatro métodos: o comparativo, da análise cultural, do grupo focal e análise documental e bibliográfica (física e digital), de fontes de trabalhos em diversas áreas. Como resultado percebemos que, embora a pesquisa tenha abrangido diferentes países, as conquistas de direitos das mulheres surdas nas políticas públicas de saúde e de segurança pública, apresentam semelhanças e diferenças na comparação entre os países.
Feminisation of Extremism as a Socio-Psychological Phenomenon
Authors: Bolysbayeva Ainura , Abikenov Zharkynbek , Bolysbayeva Aigerim , Taskymbayeva Raya , Sarsenova Assem 
Published: September 2024

Today, religious extremism, transforming and evolving, has appeared before the world community in a new form of feminist extremism. Since the radicalisation of women affects all levels of society, from everyday life to political institutions, this problem is the most pressing at present. Therefore, this study aims to make a socio-psychological portrait of a female terrorist. The article analysed video and print interviews of six women who were convicted under the article "Terrorism". In order to fully consider this problem, media materials devoted to these women were studied. The study explored the history of feminisation of extremism, its impact on society, and methods of prevention, as well as constructed a sociological portrait of a woman influenced by extremism and indicated her motives for radicalisation. The obtained data can be used to create programmes to counter extremism and terrorism, gathering information for public lectures.