bluuebunny/crossref_metadata_2025_split huggingface dataset:
Challenging nurse student selection policy: Using a lifeworld approach to explore the link between care experience and student values
Authors: Scammell Janet , Tait Desiree , White Sara , Tait Michael 
Published: July 2017

AbstractAimThis study uses a lifeworld perspective to explore beginning students’ values about nursing. Internationally, increasing care demand, a focus on targets and evidence of dehumanized care cultures have resulted in scrutiny of practitioner values. In England, selection policy dictates that prospective nursing students demonstrate person‐centred values and care work experience. However, there is limited recent evidence exploring values at programme commencement or the effect of care experience on values.DesignMixed method study.MethodsA total of 161 undergraduate nursing students were recruited in 2013 from one English university. Thematic content analysis and frequency distribution to reveal descriptive statistics were used.ResultsStatistical analysis indicated that most of the values identified in student responses were not significantly affected by paid care experience. Five themes were identified: How I want care to be; Making a difference; The value of learning; Perceived characteristics of a nurse; and Respecting our humanity. Students readily drew on their experience of living to identify person‐centred values about nursing.
Numerical Investigation of Flow Around a Multi-Element Airfoil with Hybrid RANS-LES Approaches Based on SST Model
Authors: Zhang L. , Li J. , Mou Y. F. , Zhang H. , Shi W. B. , Jin J. 
Published: July 2017

AbstractAccurate prediction of the flow around multi-element airfoil is a prerequisite for improving aerodynamic performance, but its complex flow features impose high demands on turbulence modeling. In this work, delayed detached-eddy-simulation (DDES) and zonal detached-eddy-simulation (ZDES) was applied to simulate the flow past a three-element airfoil. To investigate the effects of numerical dissipation of spatial schemes, the third-order MUSCL and the fifth-order interpolation based on modified Roe scheme were implemented. From the comparisons between the calculations and the available experimental result, third-order MUSCL-Roe can provide satisfactory mean velocity profiles, but the excessive dissipation suppresses the velocity fluctuations level and eliminates the small-scale structures; DDES cannot reproduce the separation near the trailing edge of the flap which lead to the discrepancy in mean pressure coefficients, while ZDES result has better tally with the experiment.
Reflections of faraway places: the Chalcolithic personal ornaments of Canhasan I
Authors: Baysal Emma L. 
Published: July 2017

AbstractExcavations during the 1960s of the site of Canhasan I in Karaman province in central Turkey revealed that the Chalcolithic ornaments of the region were both complex and varied. The ornaments of the site, consisting of beads (including pendants and plaques), bracelets and plugs or labrets, were made in many forms and from a variety of different materials, and thus hint at a connected world where ideas, resources and products moved from one place to another. While a catalogue of some of the artefacts has been produced previously (French 2010), this article details these ornaments and considers their temporal and geographical positions within the history of beads, bracelets and other decorative items for the first time. It explores legacies from the past, new fashions and the complicated relationships between material sources, technology, forms, style and use during a period and in an artefact category that have often been overlooked.
Funerary and votive monuments in Graeco-Roman Cilicia: Hellenistic, Roman and early Byzantine examples in the museums of Mersin and Alanya
Authors: Laflı Ergün 
Published: July 2017

AbstractIn this contribution, 13 previously unpublished grave and votive monuments are analysed, plus two boundary markers. These monuments, housed in the museums of Mersin and Alanya in Cilicia in southern Asia Minor, are both artistic and epigraphic documents. Most of them were made in this region, but three were imported from Antioch-on-the-Orontes, Pisidia and the island of Delos, as can be deduced from their iconography. These new examples from Cilicia and eastern Pamphylia offer insights into the different concepts of μνῆμα or μνημεῖον (memorial) popular in Hellenistic and Roman times throughout Asia Minor.
Artificial grammar learning in Williams syndrome and in typical development: The role of rules, familiarity, and prosodic cues
Authors: STOJANOVIK VESNA , ZIMMERER VITOR , SETTER JANE , HUDSON KERRY , POYRAZ-BILGIN ISIL , SADDY DOUG 
Published: July 2017

ABSTRACTArtificial grammar learning is an empirical paradigm that investigates basic pattern and structural processing in different populations. It can inform how higher cognitive functions, such as language use, take place. Our study used artificial grammar learning to assess how children with Williams syndrome (WS;n= 16) extract patterns in structured sequences of synthetic speech, how they compare to typically developing (TD) children (n= 60), and how prosodic cues affect learning. The TD group was divided into a group whose nonverbal abilities were within the range of the WS group, and a group whose chronological age was within the range of the WS group. TD children relied mainly on rule-based generalization when making judgments about sequence acceptability, whereas children with WS relied on familiarity with specific stimulus combinations. The TD participants whose nonverbal abilities were similar to the WS group showed less evidence of relying on grammaticality than TD participants whose chronological age was similar to the WS group. In absence of prosodic cues, the children with WS did not demonstrate evidence of learning. Results suggest that, in WS children, the transition to rule-based processing in language does not keep pace with TD children and may be an indication of differences in neurocognitive mechanisms.
Treatment of allicin improves maturation of immature oocytes and subsequent developmental ability of preimplantation embryos
Authors: Jeong Sang-Gi , Lee Seung-Eun , Park Yun-Gwi , Son Yeo-Jin , Shin Min-Young , Kim Eun-Young , Park Se-Pill 
Published: July 2017

SummaryAllicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study investigated the effects of allicin treatment on porcine oocyte maturation and developmental competence. Porcine oocytes were cultured in medium supplemented with 0 (control), 0.01, 0.1, 1, 10 or 100 μM AL, respectively, during in vitro maturation (IVM). The rate of polar body emission was higher in the 0.1 AL-treated group (74.5% ± 2.3%) than in the control (68.0% ± 2.6%) (P< 0.1). After parthenogenetic activation, the rates of cleavage and blastocyst formation were significantly higher in the 0.1 AL-treated group than in the control (P< 0.05). The reactive oxygen species level at metaphase II did not significantly differ among all groups. In matured oocytes, the expression of bothBAKandCASP3, andBIRC5was significantly lower and higher, respectively, in the 0.1 AL-treated group than in the control. Similarly, the expression ofBMP15andCCNB1, and the activity of phospho-p44/42 mitogen-activated protein kinase (MAPK), significantly increased. These results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes.
Design and Fabrication of a Three-Dimensional In Vitro System for Modeling Vascular Stenosis
Authors: Jones Rebecca S. , Chang Pin H. , Perahia Tzlil , Harmon Katrina A. , Junor Lorain , Yost Michael J. , Fan Daping , Eberth John F. , Goodwin Richard L. 
Published: July 2017

AbstractVascular stenosis, the abnormal narrowing of blood vessels, arises from defective developmental processes or atherosclerosis-related adult pathologies. Stenosis triggers a series of adaptive cellular responses that induces adverse remodeling, which can progress to partial or complete vessel occlusion with numerous fatal outcomes. Despite its severity, the cellular interactions and biophysical cues that regulate this pathological progression are poorly understood. Here, we report the design and fabrication of a three-dimensional (3D) in vitro system to model vascular stenosis so that specific cellular interactions and responses to hemodynamic stimuli can be investigated. Tubular cellularized constructs (cytotubes) were produced, using a collagen casting system, to generate a stenotic arterial model. Fabrication methods were developed to create cytotubes containing co-cultured vascular cells, where cell viability, distribution, morphology, and contraction were examined. Fibroblasts, bone marrow primary cells, smooth muscle cells (SMCs), and endothelial cells (ECs) remained viable during culture and developed location- and time-dependent morphologies. We found cytotube contraction to depend on cellular composition, where SMC-EC co-cultures adopted intermediate contractile phenotypes between SMC- and EC-only cytotubes. Our fabrication approach and the resulting artery model can serve as an in vitro 3D culture system to investigate vascular pathogenesis and promote the tissue engineering field.
β-Ginkgotides: Hyperdisulfide-constrained peptides from Ginkgo biloba
Authors: Wong Ka H. , Tan Wei Liang , Xiao Tianshu , Tam James P. 
Published: July 2017

AbstractHyperdisulfide-constrained peptides are distinguished by their high stability and diverse functions. Thus far, these peptides have been reported from animals only but their occurrence in plants are rare. Here, we report the discovery, synthesis and characterization of a hyperdisulfide-constrained peptides family of approximately 2 kDa, β-ginkgotides (β-gB1 and β-gB2) from Ginkgo biloba. Proteomic analysis showed β-ginkgotides contain 18‒20 amino acids, of which 16 residues form a conserved six-cysteine core with a highly clustered cysteine spacing of C‒CC‒C‒CC, an arrangement that has not been reported in cysteine-rich peptides. Disulfide mapping revealed a novel disulfide connectivity of CysI‒IV, CysII‒VI and CysIII‒V. Oxidative folding of synthetic β-gB1 to the native form was obtained in 70% yield. The synthetic β-gB1 displays a compact structure with no regular secondary structural elements, as determined by NMR spectroscopy. Transcriptomic analysis showed precursor βgb1 has a four-domain architecture and revealed an additional 76 β-ginkgotide-like peptides in 59 different gymnosperms, but none in angiosperms. Phylogenetic clustering analysis demonstrated β-ginkgotides belong to a new cysteine-rich peptide family. β-Ginkgotide is resistant to thermal, chemical and proteolytic degradation. Together, β-ginkgotides represent the first-in-class hyperdisulfide-constrained peptide family from plants with a novel scaffold that could be useful for engineering metabolically stable peptidyl therapeutics.
iCAVE: an open source tool for visualizing biomolecular networks in 3D, stereoscopic 3D and immersive 3D
Authors: Liluashvili Vaja , Kalayci Selim , Fluder Eugene , Wilson Manda , Gabow Aaron , Gümüş Zeynep H. 
Published: July 2017

Abstract
Visualizations of biomolecular networks assist in systems-level data exploration in many cellular processes. Data generated from high-throughput experiments increasingly inform these networks, yet current tools do not adequately scale with concomitant increase in their size and complexity. We present an open source software platform, interactome-CAVE (iCAVE), for visualizing large and complex biomolecular interaction networks in 3D. Users can explore networks (i) in 3D using a desktop, (ii) in stereoscopic 3D using 3D-vision glasses and a desktop, or (iii) in immersive 3D within a CAVE environment. iCAVE introduces 3D extensions of known 2D network layout, clustering, and edge-bundling algorithms, as well as new 3D network layout algorithms. Furthermore, users can simultaneously query several built-in databases within iCAVE for network generation or visualize their own networks (e.g., disease, drug, protein, metabolite). iCAVE has modular structure that allows rapid development by addition of algorithms, datasets, or features without affecting other parts of the code. Overall, iCAVE is the first freely available open source tool that enables 3D (optionally stereoscopic or immersive) visualizations of complex, dense, or multi-layered biomolecular networks. While primarily designed for researchers utilizing biomolecular networks, iCAVE can assist researchers in any field.
Attempting to validate the overtriage/undertriage matrix at a Level I trauma center
Authors: Davis James W. , Dirks Rachel C. , Sue Lawrence P. , Kaups Krista L. 
Published: July 2017

BACKGROUND
The Optimal Resources Document mandates trauma activation based on injury mechanism, physiologic and anatomic criteria and recommends using the overtriage/undertriage matrix (Matrix) to evaluate the appropriateness of trauma team activation. The purpose of this study was to assess the effectiveness of the Matrix method by comparing patients appropriately triaged with those undertriaged. We hypothesized that these two groups are different, and Matrix does not discriminate the needs or outcomes of these different groups of patients.

METHODS
Trauma registry data, from January 2013 to December 2015, at a Level I trauma center, were reviewed. Overtriage and undertriage rates were calculated by Matrix. Patients with Injury Severity Score (ISS) of 16 or greater were classified by activation level (full, limited, consultation), and triage category by Matrix. Patients in the limited activation and consultation groups were compared with patients with full activation by demographics, injuries, initial vital signs, procedures, delays to procedure, intensive care unit admission, length of stay, and mortality.

RESULTS
Seven thousand thirty-one patients met activation criteria. Compliance with American College of Surgeons tiered activation criteria was 99%. The Matrix overtriage rate was 45% and undertriage was 24%. Of 2,282 patients with an ISS of 16 or greater, 1,026 were appropriately triaged (full activation), and 1,256 were undertriaged. Undertriaged patients had better Glasgow Coma Scale score, blood pressure, and base deficit than patients with full activation. Intensive care unit admission, hospital stays, and mortality were lower in the undertriaged group. The undertriaged group required fewer operative interventions with fewer delays to procedure.

CONCLUSION
Despite having an ISS of 16 or greater, patients with limited activations were dissimilar to patients with full activation. Level of activation and triage are not equivalent. The American College of Surgeons Committee on Trauma full and tiered activation criteria are a robust means to have the appropriate personnel present based on the available prehospital information. Evaluation of the process of care, regardless of level of activation, should be used to evaluate trauma center performance.

LEVEL OF EVIDENCE
Therapeutic and care management, level III.
Contemporary management of subclavian and axillary artery injuries—A Western Trauma Association multicenter review
Authors: Waller Christine J. , Cogbill Thomas H. , Kallies Kara J. , Ramirez Luis D. , Cardenas Justin M. , Todd S. Rob , Chapman Kayla J. , Beckman Marshall A. , Sperry Jason L. , Anto Vincent P. , Eriksson Evert A. , Leon Stuart M. , Anand Rahul J. , Pearlstein Maura , Capano-Wehrle Lisa , Cothren Burlew Clay , Fox Charles J. , Cullinane Daniel C. , Roberts Jennifer C. , Harrison Paul B. , Berg Gina M. , Haan James M. , Lightwine Kelly 
Published: July 2017

BACKGROUND
Subclavian and axillary artery injuries are uncommon. In addition to many open vascular repairs, endovascular techniques are used for definitive repair or vascular control of these anatomically challenging injuries. The aim of this study was to determine the relative roles of endovascular and open techniques in the management of subclavian and axillary artery injuries comparing hospital outcomes, and long-term limb viability.

METHODS
A multicenter, retrospective review of patients with subclavian or axillary artery injuries from January 1, 2004, to December 31, 2014, was completed at 11 participating Western Trauma Association institutions. Statistical analysis included χ2, t-tests, and Cochran-Armitage trend tests. A p value less than 0.05 was significant.

RESULTS
Two hundred twenty-three patients were included; mean age was 36 years, 84% were men. An increase in computed tomography angiography and decrease in conventional angiography was observed over time (p = 0.018). There were 120 subclavian and 119 axillary artery injuries. Procedure type was associated with injury grade (p < 0.001). Open operations were performed in 135 (61%) patients, including 93% of greater than 50% circumference lacerations and 83% of vessel transections. Endovascular repairs were performed in 38 (17%) patients; most frequently for pseudoaneurysms. Fourteen (6%) patients underwent a hybrid procedure. Use of endovascular versus open procedures did not increase over the duration of the study (p = 0.248). In-hospital mortality rate was 10%. Graft or stent thrombosis occurred in 7% and graft or stent infection occurred in 3% of patients. Mean follow-up was 1.6 ± 2.4 years (n = 150). Limb salvage was achieved in 216 (97%) patients.

CONCLUSION
The management of subclavian and axillary artery injuries still requires a wide variety of open exposures and procedures, especially for the control of active hemorrhage from more than 50% vessel lacerations and transections. Endovascular repairs were used most often for pseudoaneurysms. Low early complication rates and limb salvage rates of 97% were observed after open and endovascular repairs.

LEVEL OF EVIDENCE
Prognostic/epidemiologic, level IV.
A naturalistic assay for measuring behavioral responses to aversive stimuli at millisecond timescale
Authors: Schoonover Carl E. , Fink Andrew J. P. , Axel Richard 
Published: July 2017

AbstractWe have designed a Virtual Burrow Assay (VBA) to detect the behavioral responses of head-fixed mice to aversive stimuli. We demonstrate its suitability for measuring novelty detection as well as aversion to both conditioned and innately aversive cues. The VBA simulates a scenario in which a mouse, poised at the threshold of its burrow, evaluates whether to remain exposed to potential threats outside or to retreat inside an enclosure. When presented with aversive stimuli, mice exhibit a stereotyped retreat whose onset is determined by measuring the position of a moveable burrow. This withdrawal, which requires no training, is characterized by an abrupt transition that unfolds within milliseconds—a timescale similar to that of neuronal dynamics, permitting direct comparison between the two. The assay is compatible with standard electrophysiological and optical methods for measuring and perturbing neuronal activity.
ComparingCaenorhabditis elegansgentle and harsh touch response behavior using a multiplexed hydraulic microfluidic device
Authors: McClanahan Patrick D. , Xu Joyce H. , Fang-Yen Christopher 
Published: July 2017

AbstractThe roundwormCaenorhabditis elegansis an important model system for understanding the genetics and physiology of touch. Classical assays forC. eleganstouch, which involve manually touching the animal with a probe and observing its response, are limited by their low throughput and qualitative nature. We developed a microfluidic device in which several dozen animals are subject to spatially localized mechanical stimuli with variable amplitude. The device contains 64 sinusoidal channels through which worms crawl, and hydraulic valves that deliver touch stimuli to the worms. We used this assay to characterize the behavioral responses to gentle touch stimuli and the less well studied harsh (nociceptive) touch stimuli. First, we measured the relative response thresholds of gentle and harsh touch. Next, we quantified differences in the receptive fields between wild type worms and a mutant with non-functioning posterior touch receptor neurons. We showed that under gentle touch the receptive field of the anterior touch receptor neurons extends into the posterior half of the body. Finally, we found that the behavioral response to gentle touch does not depend on the locomotion of the animal immediately prior to the stimulus, but does depend on the location of the previous touch. Responses to harsh touch, on the other hand, did not depend on either previous velocity or stimulus location. Differences in gentle and harsh touch response characteristics may reflect the different innervation of the respective mechanosensory cells. Our assay will facilitate studies of mechanosensation, sensory adaptation, and nociception.
ScreenCube: A 3D Printed System For Rapid and Cost-Effective Chemical Screening in Adult Zebrafish
Authors: Monstad-Rios Adrian T. , Watson Claire J. , Kwon Ronald Y. 
Published: July 2017

ABSTRACTPhenotype-based small molecule screens in zebrafish embryos and larvae have been successful in accelerating pathway and therapeutic discovery for diverse biological processes. Yet, the application of chemical screens to adult physiologies has been relatively limited due to additional demands on cost, space, and labor associated with screens in adult animals. Here, we present a 3D printed system and methods for intermittent drug dosing that enable rapid and cost-effective chemical administration in adult zebrafish. Using pre-filled screening plates, the system enables dosing of 96 fish in ˜3min, with a tenfold-reduction in drug quantity compared to that used in previous chemical screens in adult zebrafish. We characterize water quality kinetics during immersion in the system, and use these kinetics to rationally design intermittent dosing regimens that result in 100% fish survival. As a demonstration of system fidelity, we show the potential to identify two known chemical inhibitors of adult tail fin regeneration, cyclopamine and dorsomorphin. By developing methods for rapid and cost-effective chemical administration in adult zebrafish, this study expands the potential for small molecule discovery in post-embryonic models of development, disease, and regeneration.
Mechanism of genome interrogation: How CRISPR RNA-guided Cas9 proteins locate specific targets on DNA
Authors: Shvets A. A. , Kolomeisky A. B. 
Published: July 2017

AbstractThe ability to precisely edit and modify genome opens endless opportunities to investigate fundamental properties of living systems as well as to advance various medical techniques and bioengineering applications. This possibility is now close to reality due to a recent discovery of the adaptive bacterial immune system, which is based on clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) that utilize RNA to find and cut the double-stranded DNA molecules at specific locations. Here we develop a quantitative theoretical approach to analyze the mechanism of target search on DNA by CRISPR RNA-guided Cas9 proteins, which is followed by a selective cleavage of nucleic acids. It is based on a discrete-state stochastic model that takes into account the most relevant physical-chemical processes in the system. Using a method of first-passage processes, a full dynamic description of the target search is presented. It is found that the location of specific sites on DNA by CRISPR Cas9 proteins is governed by binding first to protospacer adjacent motif (PAM) sequences on DNA, which is followed by reversible transitions into DNA interrogation states. In addition, the search dynamics is strongly influenced by the off-target cutting. Our theoretical calculations allows us to explain the experimental observations and to give experimentally testable predictions. Thus, the presented theoretical model clarifies some molecular aspects of the genome interrogation by CRISPR RNA-guided Cas9 proteins.Insert Received for publication Date and in final form Date.