bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic profiling of hemp bast fibres at different developmental stages
Authors: Guerriero Gea , Behr Marc , Legay Sylvain , Mangeot-Peter Lauralie , Zorzan Simone , Ghoniem Mohammad , Hausman Jean-Francois 
Published: July 2017

AbstractBast fibres are long extraxylary cells which mechanically support the phloem and they are divided into xylan- and gelatinous-type, depending on the composition of their secondary cell walls. The former, typical of jute/kenaf bast fibres, are characterized by the presence of xylan and a high degree of lignification, while the latter, found in tension wood, as well as flax, ramie and hemp bast fibres, have a high abundance of crystalline cellulose. During their differentiation, bast fibres undergo specific developmental stages: the cells initially elongate rapidly by intrusive growth, subsequently they cease elongation and start to thicken. The goal of the present study is to provide a transcriptomic close-up of the key events accompanying bast fibre development in textile hemp (Cannabis sativa L.), a fibre crop of great importance. Bast fibres have been sampled from different stem regions. The developmental stages corresponding to active elongation and cell wall thickening have been studied using RNA-Seq. The results show that the fibres sampled at each stem region are characterized by a specific transcriptomic signature and that the major changes in cell wall-related processes take place at the internode containing the snap point. The data generated also identify several interesting candidates for future functional analysis.
Effect of Li Termination on the Electronic and Hydrogen Storage Properties of Linear Carbon Chains: A TAO-DFT Study
Authors: Seenithurai Sonai , Chai Jeng-Da 
Published: July 2017

AbstractAccurate prediction of the electronic and hydrogen storage properties of linear carbon chains (C
n
) and Li-terminated linear carbon chains (Li2C
n
), with n carbon atoms (n = 5–10), has been very challenging for traditional electronic structure methods, due to the presence of strong static correlation effects. To meet the challenge, we study these properties using our newly developed thermally-assisted-occupation density functional theory (TAO-DFT), a very efficient electronic structure method for the study of large systems with strong static correlation effects. Owing to the alteration of the reactivity of C
n
and Li2C
n
with n, odd-even oscillations in their electronic properties are found. In contrast to C
n
, the binding energies of H2 molecules on Li2C
n
are in (or close to) the ideal binding energy range (about 20 to 40 kJ/mol per H2). In addition, the H2 gravimetric storage capacities of Li2C
n
are in the range of 10.7 to 17.9 wt%, satisfying the United States Department of Energy (USDOE) ultimate target of 7.5 wt%. On the basis of our results, Li2C
n
can be high-capacity hydrogen storage materials that can uptake and release hydrogen at temperatures well above the easily achieved temperature of liquid nitrogen.
PDK1 plays a vital role on hematopoietic stem cell function
Authors: Hu Tianyuan , Li Cong , Wang Le , Zhang Yingchi , Peng Luyun , Cheng Hui , Chu Yajing , Wang Weili , Ema Hideo , Gao Yingdai , Ju Zhenyu , Yang Zhongzhou , Wang Xiaomin , Cheng Tao , Yuan Weiping 
Published: July 2017

Abstract3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a pivotal regulator in the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway that have been shown to play key roles in the functional development of B and T cells via activation of AGC protein kinases during hematopoiesis. However, the role of PDK1 in HSCs has not been fully defined. Here we specifically deleted the PDK1 gene in the hematopoietic system and found that PDK1-deficient HSCs exhibited impaired function and defective lineage commitment abilities. Lack of PDK1 caused HSCs to be less quiescent and to produce a higher number of phenotypic HSCs and fewer progenitors. PDK1-deficient HSCs were also unable to reconstitute the hematopoietic system. Notably, HSC function was more dependent on PDK1 than on mTORC2, which indicates that PDK1 plays a dominant role in the Akt-mediated regulation of HSC function. PDK1-deficient HSCs also exhibited reduced ROS levels, and treatment of PDK1-deficient HSCs with L-butathioninesulfoximine in vitro elevated the low ROS level and promoted colony formation. Therefore, PDK1 appears to contribute to HSC function partially via regulating ROS levels.
Ticks infected via co-feeding transmission can transmit Lyme borreliosis to vertebrate hosts
Authors: Belli Alessandro , Sarr Anouk , Rais Olivier , Rego Ryan O. M. , Voordouw Maarten J. 
Published: July 2017

AbstractVector-borne pathogens establish systemic infections in host tissues to maximize transmission to arthropod vectors. Co-feeding transmission occurs when the pathogen is transferred between infected and naive vectors that feed in close spatiotemporal proximity on a host that has not yet developed a systemic infection. Borrelia afzelii is a tick-borne spirochete bacterium that causes Lyme borreliosis (LB) and is capable of co-feeding transmission. Whether ticks that acquire LB pathogens via co-feeding are actually infectious to vertebrate hosts has never been tested. We created nymphs that had been experimentally infected as larvae with B. afzelii via co-feeding or systemic transmission, and compared their performance over one complete LB life cycle. Co-feeding nymphs had a spirochete load that was 26 times lower than systemic nymphs but both nymphs were highly infectious to mice (i.e., probability of nymph-to-host transmission of B. afzelii was ~100%). The mode of transmission had no effect on the other infection phenotypes of the LB life cycle. Ticks that acquire B. afzelii via co-feeding transmission are highly infectious to rodents, and the resulting rodent infection is highly infectious to larval ticks. This is the first study to show that B. afzelii can use co-feeding transmission to complete its life cycle.
Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities
Authors: Zakharzhevskaya Natalya B. , Vanyushkina Anna A. , Altukhov Ilya A. , Shavarda Aleksey L. , Butenko Ivan O. , Rakitina Daria V. , Nikitina Anastasia S. , Manolov Aleksandr I. , Egorova Alina N. , Kulikov Eugene E. , Vishnyakov Innokentii E. , Fisunov Gleb Y. , Govorun Vadim M. 
Published: July 2017

AbstractNumerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of the most popular topics for research. For example, the outer membrane vesicles (OMVs) of Bacteroides fragilis play an important nutritional role with respect to other microorganisms and promote anti-inflammatory effects on immune cells. However, toxigenic B. fragilis (ETBF) contributes to bowel disease, even causing colon cancer. If nontoxigenic B. fragilis (NTBF) vesicles exert a beneficial effect on the intestine, it is likely that ETBF vesicles can be utilized for potential pathogenic implementation. To confirm this possibility, we performed comparative proteomic HPLC-MS/MS analysis of vesicles isolated from ETBF and NTBF. Furthermore, we performed, for the first time, HPLC-MS/MS and GS-MS comparative metabolomic analysis for the vesicles isolated from both strains with subsequent reconstruction of the vesicle metabolic pathways. We utilized fluxomic experiments to validate the reconstructed biochemical reaction activities and finally observed considerable difference in the vesicle proteome and metabolome profiles. Compared with NTBF OMVs, metabolic activity of ETBF OMVs provides their similarity to micro reactors that are likely to be used for long-term persistence and implementing pathogenic potential in the host.
Single-cell transcriptomics of East-Asian pancreatic islets cells
Authors: Dorajoo Rajkumar , Ali Yusuf , Tay Vanessa S. Y. , Kang Jonathan , Samydurai Sudhagar , Liu Jianjun , Boehm Bernhard O. 
Published: July 2017

AbstractSingle-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis.
Generation of path-polarization hyperentanglement using quasi-phase-matching in quasi-periodic nonlinear photonic crystal
Authors: He Guangqiang , Zhu Chengrui , Jiang Yao , Ren Jie , Guo Ying , Jing Jietai 
Published: July 2017

AbstractA compact scheme for the generation of path-polarization entangled photon pairs is proposed by using a quasi-periodic nonlinear photonic crystal to simultaneously accomplish four spontaneous parametric down-conversion processes. Moreover, we report experimental scheme to measure the polarization entanglement and path entanglement separately and theoretically get numerical results that verify some predictions about the hyperentanglement. This method can be expanded for the generation of multi-partite and two-photon path-polarization hyperentanglement in a single quasi-periodic nonlinear photonic crystal structure. This compact quantum light source can be used as a significant ingredient in quantum information science.
Genome-wide analysis of long noncoding RNA profiling in PRRSV-infected PAM cells by RNA sequencing
Authors: Zhang Jing , Sun Pu , Gan Lipeng , Bai Weijie , Wang Zhijia , Li Dong , Cao Yimei , Fu Yuanfang , Li Pinghua , Bai Xingwen , Ma Xueqing , Bao Huifang , Chen Yingli , Liu Zaixin , Lu Zengjun 
Published: July 2017

AbstractPorcine reproductive and respiratory syndrome (PRRS) is a major threat to the global swine industry and causes tremendous economic losses. Its causative agent, porcine reproductive and respiratory syndrome virus (PRRSV), primarily infects immune cells, such as porcine alveolar macrophages and dendritic cells. PRRSV infection results in immune suppression, antibody-dependent enhancement, and persistent infection. Highly pathogenic strains in China cause high fever and severe inflammatory responses in the lungs. However, the pathogenesis of PRRSV is still not fully understood. In this study, we analysed the long noncoding RNA (lncRNA) and mRNA expression profiles of the HP-PRRSV GSWW15 and the North American strain FL-12 in infected porcine alveolar macrophages (PAMs) at 12 and 24 hours post-infection. We predicted 12,867 novel lncRNAs, 299 of which were differentially expressed after viral infection. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analyses of the genes adjacent to lncRNAs showed that they were enriched in pathways related to viral infection and immune response, indicating that lncRNAs might play regulatory roles in virus-host interactions. Our study provided information about lncRNAs in the porcine immune system and offers new insights into the pathogenic mechanism of PRRSV infection and novel antiviral therapy development.
Transgenerational inheritance of susceptibility to diabetes-induced male subfertility
Authors: Pavlinkova Gabriela , Margaryan Hasmik , Zatecka Eva , Valaskova Eliska , Elzeinova Fatima , Kubatova Alena , Bohuslavova Romana , Peknicova Jana 
Published: July 2017

AbstractMale infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality. The testicular tissue of diabetic males showed significant tissue damage, disruption of meiotic progression, and changes in the expression of genes encoding proteins important for spermiogenesis. Paternal diabetes altered sperm quality and expression pattern in the testes in offspring of two subsequent generations. Our study revealed that paternal diabetes increased susceptibility to infertility in offspring through gametic alternations. Our data also provide a mechanistic basis for transgenerational inheritance of diabetes-associated pathologies since protamines may be involved in epigenetic regulations.
Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
Authors: Fukuchi Mamoru , Izumi Hironori , Mori Hisashi , Kiyama Masahiro , Otsuka Satoshi , Maki Shojiro , Maehata Yosuke , Tabuchi Akiko , Tsuda Masaaki 
Published: July 2017

AbstractBrain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing various neural functions including memory consolidation. Alterations of BDNF levels in the brain are associated with neurodegenerative and neuropsychiatric disorders. Therefore, it is important to understand how levels of BDNF are controlled. Recently we generated a novel transgenic mouse strain, termed the Bdnf-Luciferase transgenic (Bdnf-Luc Tg) mouse, to monitor changes in Bdnf expression. In the present study, we detected the bioluminescence signal from living Bdnf-Luc Tg mice after intraperitoneal administration of d-luciferin. Despite high levels of Bdnf expression in the brain, it was difficult to detect a signal from the brain region, probably because of its poorly penetrable (short-wavelength) bioluminescence. However, we could detect the changes in the bioluminescence signal in the brain region using a luciferin analogue generating a near-infrared wavelength of bioluminescence. We also found a strong correlation between increases in body weight and bioluminescence signal in the abdominal region of Tg mice fed a high-fat diet. These results show that changes in Bdnf expression can be visualized using living mice, and that the Tg mouse could be a powerful tool for clarification of the role of Bdnf expression in pathophysiological and physiological conditions.
Tree functional types simplify forest carbon stock estimates induced by carbon concentration variations among species in a subtropical area
Authors: Wu Huili , Xiang Wenhua , Fang Xi , Lei Pifeng , Ouyang Shuai , Deng Xiangwen 
Published: July 2017

AbstractForests contain one of the world’s largest carbon (C) pools and represent opportunities for cost-effective climate change mitigation through programmes such as the United Nations-led “Reducing Emissions from Deforestation and Forest Degradation” Programme (REDD). Generic estimates for the conversion of forest biomass into C stock are not sufficiently accurate for assessing the utility of harvesting forest to offset carbon dioxide emissions, currently under consideration by the REDD Programme. We examined the variation in C concentration among tree species and tree functional types (classified based on leaf morphological and phenological traits) in a subtropical forest and evaluated the effects of these variations on stand-level estimations of C stock. This study was conducted in the Paiyashan Forest State Farm and the Dashanchong Forest Park, Hunan Province, China. C concentrations differed significantly among tree species (P < 0.0001) and were significantly higher in gymnosperm than angiosperm species. Estimations of stand C stocks were similar using either functional types or species- and tissue-specific C concentrations. The use of functional type classification to estimate stand C stock is an effective tool for implementing C sequestration trade and C credit programmes and the UN-REDD Programme in subtropical forests.
5-LO inhibition ameliorates palmitic acid-induced ER stress, oxidative stress and insulin resistance via AMPK activation in murine myotubes
Authors: Kwak Hyun Jeong , Choi Hye-Eun , Cheon Hyae Gyeong 
Published: July 2017

AbstractLeukotriene B4 (LTB4) production via the 5-lipoxygenase (5-LO) pathway contributes to the development of insulin resistance in adipose and hepatic tissues, but the role of LTB4 in skeletal muscle is relatively unknown. Here, the authors investigated the role of LTB4 in C2C12 myotubes in palmitic acid (PA)-induced ER stress, inflammation and insulin resistance. PA (750 μM) evoked lipotoxicity (ER stress, oxidative stress, inflammation and insulin resistance) in association with LTB4 production. 5-LO inhibition reduced all the lipotoxic effects induced by PA. On the other hand, PA did not induce cysteinyl leukotrienes (CysLTs), which themselves had no effect on ER stress and inflammation. The beneficial effects of 5-LO suppression from PA-induced lipotoxicity were related with AMPK activation. In ob/ob mice, once daily oral administration of zileuton (50, 100 mg/kg) for 5 weeks improved insulin resistance, increased AMPK phosphorylation, and reduced LTB4 and ER stress marker expression in skeletal muscle. These results show that 5-LO inhibition by either zileuton or 5-LO siRNA protects C2C12 myotubes from PA-induced lipotoxicity, at least partly via AMPK activation, and suggest that the in vivo insulin-sensitizing effects of zileuton are in part attributable to its direct action on skeletal muscle via LTB4 downregulation followed by AMPK activation.
Synthesis of nanobelt-like 1-dimensional silver/nanocarbon hybrid materials for flexible and wearable electronics
Authors: Han Joong Tark , Jang Jeong In , Cho Joon Young , Hwang Jun Yeon , Woo Jong Seok , Jeong Hee Jin , Jeong Seung Yol , Seo Seon Hee , Lee Geon-Woong 
Published: July 2017

AbstractMost synthetic processes of metallic nanostructures were assisted by organic/inorganic or polymeric materials to control their shapes to one-dimension or two-dimension. However, these additives have to be removed after synthesis of metal nanostructures for applications. Here we report a straightforward method for the low-temperature and additive-free synthesis of nanobelt-like silver nanostructures templated by nanocarbon (NC) materials via bio-inspired shape control by introducing supramolecular 2-ureido-4[1H]pyrimidinone (UPy) groups into the NC surface. The growth of the Ag nanobelt structure was found to be induced by these UPy groups through observation of the selective formation of Ag nanobelts on UPy-modified carbon nanotubes and graphene surfaces. The synthesized NC/Ag nanobelt hybrid materials were subsequently used to fabricate the highly conductive fibres (>1000S/cm) that can function as a conformable electrode and highly tolerant strain sensor, as well as a highly conductive and robust paper (>10000S/cm after thermal treatment).
Raman scattering excitation spectroscopy of monolayer WS2
Authors: Molas Maciej R. , Nogajewski Karol , Potemski Marek , Babiński Adam 
Published: July 2017

AbstractResonant Raman scattering is investigated in monolayer WS2 at low temperature with the aid of an unconventional technique, i.e., Raman scattering excitation (RSE) spectroscopy. The RSE spectrum is made up by sweeping the excitation energy, when the detection energy is fixed in resonance with excitonic transitions related to either neutral or charged excitons. We demonstrate that the shape of the RSE spectrum strongly depends on the selected detection energy. The resonance of outgoing light with the neutral exciton leads to an extremely rich RSE spectrum, which displays several Raman scattering features not reported so far, while no clear effect on the associated background photoluminescence is observed. Instead, when the outgoing photons resonate with the negatively charged exciton, a strong enhancement of the related emission occurs. Presented results show that the RSE spectroscopy can be a useful technique to study electron-phonon interactions in thin layers of transition metal dichalcogenides.
Bi-directional high speed domain wall motion in perpendicular magnetic anisotropy Co/Pt double stack structures
Authors: Sethi P. , Krishnia S. , Gan W. L. , Kholid F. N. , Tan F. N. , Maddu R. , Lew W. S. 
Published: July 2017

AbstractWe report bi-directional domain wall (DW) motion along and against current flow direction in Co/Pt double stack wires with Ta capping. The bi-directionality is achieved by application of hard-axis magnetic field favoring and opposing the Dzyloshinskii-Moriya interaction (DMI), respectively. The speed obtained is enhanced when the hard-axis field favors the DMI and is along the current flow direction. Co/Pt double stack is a modification proposed for the high spin-orbit torque strength Pt/Co/Ta stack, to improve its thermal stability and perpendicular magnetic anisotropy (PMA). The velocity obtained reduces with increase in Pt spacer thickness due to reduction in DMI and enhances on increasing the Ta capping thickness due to higher SOT strength. The velocity obtained is as high as 530 m/s at a reasonable current density of 1 × 1012 A/m2 for device applications. The low anisotropy of the device coupled with the application of hard-axis field aids the velocity enhancement by preventing Walker breakdown.