bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome features of moderately halophilic polyhydroxyalkanoate-producing Yangia sp. CCB-MM3
Authors: Lau Nyok-Sean , Sam Ka-Kei , Amirul Abdullah Al-Ashraf 
Published: January 2017

AbstractYangia sp. CCB-MM3 was one of several halophilic bacteria isolated from soil sediment in the estuarine Matang Mangrove, Malaysia. So far, no member from the genus Yangia, a member of the Rhodobacteraceae family, has been reported sequenced. In the current study, we present the first complete genome sequence of Yangia sp. strain CCB-MM3. The genome includes two chromosomes and five plasmids with a total length of 5,522,061 bp and an average GC content of 65%. Since a different strain of Yangia sp. (ND199) was reported to produce a polyhydroxyalkanoate copolymer, the ability for this production was tested in vitro and confirmed for strain CCB-MM3. Analysis of its genome sequence confirmed presence of a pathway for production of propionyl-CoA and gene cluster for PHA production in the sequenced strain. The genome sequence described will be a useful resource for understanding the physiology and metabolic potential of Yangia as well as for comparative genomic analysis with other Rhodobacteraceae.
ELIXIR pilot action: Marine metagenomics – towards a domain specific set of sustainable services
Authors: Robertsen Espen Mikal , Denise Hubert , Mitchell Alex , Finn Robert D. , Bongo Lars Ailo , Willassen Nils Peder 
Published: January 2017

Metagenomics, the study of genetic material recovered directly from environmental samples, has the potential to provide insight into the structure and function of heterogeneous microbial communities. There has been an increased use of metagenomics to discover and understand the diverse biosynthetic capacities of marine microbes, thereby allowing them to be exploited for industrial, food, and health care products. This ELIXIR pilot action was motivated by the need to establish dedicated data resources and harmonized metagenomics pipelines for the marine domain, in order to enhance the exploration and exploitation of marine genetic resources. In this paper, we summarize some of the results from the ELIXIR pilot action “Marine metagenomics – towards user centric services”.
Effectiveness of amitriptyline for treating functional dyspepsia in adolescents
Authors: Mustawa Indra , Supriatmo Supriatmo , Hakimi Hakimi , Sinuhaji Atan Baas 
Published: January 2017

Background Functional dyspepsia is common among adolescents. Pain reduces children’s quality of life, psychosocial functioning, and school attendance. Amitriptyline is assumed to be one of the alternative treatments in functional dyspepsia.Objective To investigate the effectiveness of amytriptyline as a treatment for functional dyspepsia in adolescents.Methods We conducted a randomized, single-blind, controlled trial from January to March 2011 in junior and senior high school students in Dobo City, Aru Island District, Maluku Province. Adolescents suffering from functional dyspepsia and who fulfilled the inclusion criteria were eligible for the study. Subjects were randomized into two groups. Each group received 10 mg (for body weight < 35 kg) or 20 mg (for body weight ≥ 35 kg) amitriptyline or placebo once per day for 28 days. Pain frequency was measured in terms of abdominal pain episodes per month, and duration was measured in minutes. Data were analyzed using t-test.Results Eighty-eight students participated in this study: the amitriptyline group (43 subjects) and the placebo group (45 subjects). There were no statistically significant differences between the amitriptyline and placebo groups in frequency (P=0.777; 95%CI -0.846 to 1.129) or duration (P=0.728) of abdominal pain after treatment.Conclusion Amitriptyline is not more effective than placebo for treating functional dyspepsia in adolescents.
Comparison of ventilation parameters and blood gas analysis in mechanically-ventilated children who received chest physiotherapy and suctioning vs. suctioning alone
Authors: Elizabeth Monalisa , Yoel Chairul , Ali Muhammad , Loebis M. Sjabroeddin , Arifin Hasanul , Sianturi Pertin 
Published: January 2017

Background Chest physiotherapy and suctioning are routine methods for airway clearance in mechanically-ventilated children. However, chest physiotherapy has not been confirmed to affect ventilation parameters, such as tidal volume (TV), peak inflation pressure (PIP), peak inspiratory flow (PIF), and peak expiratory flow (PEF), as well as blood gas analysis (BGA) values in pediatric intensive care unit (PICU) patients.Objective To determine the efficacy of chest physiotherapy and suctioning vs. suctioning alone for improving the mechanical ventilation parameters and BGA.Methods This randomized, single-blind, clinical trial was conducted from November 2012 to June 2013 in the PICU at Haji Adam Malik Hospital, Medan. A total of 40 mechanically-ventilated pediatric patients were enrolled and divided into either the chest physiotherapy and suctioning group (24 subjects) or the suctioning alone group (16 subjects). Subjects underwent treatment, followed by monitoring of their ventilation parameters and blood gas analyses. Data were analyzed by independent t-test and Mann-Whitney test.Results Subjects comprised of 23 boys and 17 girls, with an age range of 1–204 months. After the respective treatments (chest physiotherapy and suctioning vs. suctioning alone), the ventilation parameters were as follows: median TV (60.0 vs. 56.5 mL, respectively; P=0.838), median PEF (10.4 vs. 10.8 I/s, respectively; P=0.838), median PIF (7.4 vs. 8.2 I/s, respectively; P=0.469), and mean PIP (17.3 vs. 15.6 cmH2O, respectively; P=0.23). The BGA values were: median pH (7.4 vs. 7.3, respectively; P=0.838), median pCO2 (38.4 vs. 36.2 mmHg, respectively; P=1.000), mean pO2 (136.6 vs. 139.2 mmHg, respectively; P=0.834), median HCO3 (20.4 vs. 22.7 mmol/L, respectively; P=0.594), median TCO2 (22.0 vs. 23.7 mmol/L, respectively; P=0.672), mean BE (-4.3 vs. -3.1 mmol/L, respectively; P=0.629), and median O2 saturation (98.5 vs. 98.3 %, respectively; P=0.967).Conclusion In mechanically-ventilated children in the PICU, ventilation parameters and BGA values are not significantly different between subjects who received both chest physiotherapy and suctioning and those who received suctioning alone.
Faktor Prognosis Derajat Keparahan Infeksi Dengue
Authors: Yulianto Arie , Laksono Ida Safitri , Juffrie Mohammad 
Published: January 2017

Latar belakang. Infeksi virus dengue (IVD) bersifat akut dan dinamis, perjalanan klinisnya terkadang sulit diprediksi sehingga berakibat keterlambatan pengelolaan. Maka perlu diteliti parameter klinis dan laboratoris di fase kritis / defervescence untuk memprediksi derajat keparahan infeksi dengue.Tujuan. Mengetahui parameter klinis dan laboratoris sebagai faktor prognosis derajat keparahan infeksi dengue.Metode. Penelitian kohort retrospektif, menggunakan data rekam medik pasien anak IVD yang dirawat di RSUP Dr. Sardjito Yogyakarta dari Januari 2014 – Desember 2015. Faktor prognosis yang diteliti adalah usia, jenis kelamin, tanda klinis warning signs, serta laboratorium. Kriteria eksklusi adalah adanya penyakit hematologi dan penyakit jantung bawaan. Analisis statistik denganmetode regresi logistik.Hasil. Di antara 188 pasien yang memenuhi kriteria penelitian, 56 (30%) didiagnosis demam dengue (DD), 58 (31%) demam berdarah dengue derajat 1 – 2 (DBD), dan 74 (39%) sindrom syok dengue (SSD). Analisis multivariat menunjukkan nyeri perut (OR 5,06, IK 95%: 1,72;14,87), hepatomegali >2 cm (OR 7,57, IK 95%: 2,86;20,02), hematokrit >45% (OR 5,10, IK 95%: 1,74;14,95), dan trombosit ≤50.000/uL (OR 17,80, IK 95%: 3,78;83,80) merupakan faktor prognosis independen derajat keparahan infeksi dengueKesimpulan. Nyeri perut, hepatomegali >2 cm, hematokrit >45% dan trombosit ≤50.000/uL di fase defervescence merupakan faktor prognosis independen terjadinya infeksi dengue yang lebih berat (DBD dan SSD).
Kelainan Kardiovaskular pada Anak dengan Berbagai Stadium Penyakit Ginjal Kronik
Authors: Pabuti Aumas , Sekarwana Nanan , Trihono Partini P 
Published: January 2017

Latar belakang. Penyakit kardiovaskular (PKV) merupakan penyebab orbiditas dan mortalitas tersering pada penyakit ginjal kronik (PGK) anak. Hipertrofi ventrikel kiri (LVH) dan disfungsi diastolik paling awal terlihat.Tujuan. Mengetahui proporsi PKV pada PGK stadium 1, hubungan stadium PGK dengan LVH, dan disfungsi jantung.Metode. Penelitian potong lintang analitik komparatif 26 subjek PGK anak di RS M Djamil Padang/ RS Hasan Sadikin Bandung. Dilakukan pemeriksaan urinalisis, hematologi rutin, ureum, kreatinin (eLFG), EKG, foto toraks, ekokardiografi. LVH dengan ekokardiografi bila left ventricular mass index (LVMI) >persentil 95 (38g/h2,7). Uji stastistik bermakna bila p<0,05.Hasil. Rerata umur subjek 9,1(3,8) tahun. LVH pada 1 dari 3 subjek PGK stadium 1 dan 61,5% pada PGK seluruh stadium, terbanyak stadium 5. Tidak terdapat hubungan bermakna stadium PGK dengan LVH (p=0,055), disfungsi diastolik (p=0,937) dan disfungsi sistolik (p=0,929).Kesimpulan. Pada PGK stadium 1 ditemukan LVH dan disfungsi diastolik. Tidak terdapat hubungan antara stadium PGK dengan LVH dan disfungsi jantung.
Status Nutrisi sebagai Faktor Risiko Sindrom Syok Dengue
Authors: Buntubatu Sarah , Arguni Eggi , Indrawanti Ratni , Laksono Ida Safitri , Prawirohartono Endy P. 
Published: January 2017

Latar belakang. Risiko kematian pada anak dengan sindrom syok dengue (SSD) tinggi. Obesitas diduga sebagai faktor risiko SSD tetapi hasil penelitian sebelumnya masih kontroversial.Tujuan. Mengevaluasi overweight atau obes sebagai faktor risiko SSD pada anak.Metode. Penelitian case control pada anak (0-18 tahun) yang dirawat di RSUP Dr. Sardjito Yogyakarta dari bulan April 2012 sampai Maret 2015 dengan infeksi dengue, yang ditegakkan berdasarkan kriteria WHO 2011 dan hasil uji serologi darah atau NS1. Analisis statistik dikerjakan dengan analisis multivariat dan stratifikasi. Kemaknaan faktor risiko dilaporkan sebagai odds ratio (OR) denganinterval kepercayaan 95%.Hasil. Dilaporkan 264 anak terdiri dari 88 kasus (SSD) dan 176 kontrol (non SSD) diikutkan dalam penelitian ini. Anak dengan overweight atau obesitas mempunyai risiko SSD 2,29 kali dibanding bukan overweight atau obes OR=2,29; IK95%:1,24-4,22) dan perempuan mempunyai risiko SSD 1,84 kali lebih tinggi dibanding laki-laki (OR=1,84; IK95%:1,08-3,14). Jumlah trombositmerupakan modifier, yaitu anak overweight atau obes dengan angka trombosit <20.000/μL mempunyai risiko 3,26 kali dibanding anak dengan SSD dengan overweight atau obes dengan angka trombosit ≥20.000/μL (OR=3,26;IK95%:1,22-8,72).Kesimpulan. Overweight atau obes dan jenis kelamin perempuan merupakan faktor risiko untuk SSD dan jumlah trombosit <20.000 /μL merupakan modifier.
Prevalence of malocclusion in people with disabilities
Authors: Cuoghi Osmar Aparecido , Faria Lorraine Perciliano , Micheletti Kelly Regina , Miranda-Zamalloa Yesselin Margot , De Mendonça Marcos Rogério 
Published: January 2017

Objective: To evaluate the prevalence of malocclusion in patients with disabilities. Material and Methods: The sample consisted in 98 patients of who had a previous diagnosis of disability. Patientes were divided into three groups: Intelectual Disabilities (ID), Cerebral Palsy (CP) and Down Syndrome (DS). They were evaluated according to Angle malocclusion classification, presence or absence of posterior crossbite, anterior crossbite and anterior open bite. Statistical analysis of the data was performed using the Kruskal Wallis test at 5% significance level. Results: Class II malocclusion was more frequent in the three groups (CP 45.45%, ID 40.43% and DS 50.00%); the anterior and the posterior crossbite were more prevalent in DS (p < 0.0001). The anterior open bite was more prevalent in CP (p < 0.0001). Conclusion: Patients with disabilities have high rate of malocclusion and understanding this condition is essential to establish appropriate treatment.Keywords: Orthodontics; Malocclusion; People with disabilities.
El fondo del paisaje: una pauta de lectura paisajística del cine ruso y soviético a partir del concepto de fondo como categoría estética
Authors: Muguiro Carlos 
Published: January 2017

La idea de fondo (horizonte) es consustancial al nacimiento del paisaje en Occidente, un concepto que el cine hereda y asume desde sus orígenes. Este artículo plantea específicamente la posibilidad de acercarse, a partir de esta figura, a la representación de la naturaleza en el cine ruso y soviético. El artículo desarrolla la idea de que el fondo es una categoría estética en sí misma a partir de la cual se puede establecer una pauta de análisis diacrónico e histórico de dicha cinematografía. Desde un punto de vista metódologico, el artículo se basa en la doble distinción planteada por Maurizia Natali entre la figura y el fondo y el fondo del fondo. Temporalmente, se centra específicamente en las virtualidades del fondo hasta la consolidación del Realismo Socialista a mediados de los años 30 del pasaso siglo.
A revisitação da paisagem nas longas-metragens de Manuel Guimarães
Authors: Vieira da Silva Tiago 
Published: January 2017

A obra de Manuel Guimarães destaca-se no cinema português como uma nova e singular forma de representar a realidade nacional até então muito “forjada” segundo os princípios e valores prezados pelo Estado Novo. Neste contexto, a paisagem revela-se um elemento crucial na subversão das relações humanas com o território, e na própria representação do ambiente físico. O cinema de Manuel Guimarães distingue-se muito através do redimensionamento espacial que, ao conceder novos significados ao território, vai também apresentar galerias de personagens que se diferenciam das que preenchem o cinema do período. A revisitação da paisagem nas longas-metragens de Manuel Guimarães propõe-se analisar a maneira como o cineasta recodifica as paisagens no seu cinema, numa tentativa de contestar a utopia estabelecida pelo regime.
Interpreting the Pore Structure of Hydrating Cement Phases Through a Synergistic Use of the Powers-Brownyard Model, Hydration Kinetics, and Thermodynamic Calculations
Authors: Jafari Azad V. , Suraneni P. , Isgor O. B. , Weiss W. J. 
Published: January 2017

Abstract
The Powers-Brownyard model is an analytical tool that is widely used to quantify the volume of gel solids, gel pores, capillary water, and unhydrated cement during the hydration process. These parameters, specifically the capillary and gel pore volumes, are related to important hardened concrete properties, such as shrinkage, freeze-thaw, transport, and strength. While the Powers-Brownyard model has many applications, it does not provide detailed solid phase assemblage information, and it can only be used for ordinary portland cement. In contrast, thermodynamic modeling can predict solid phase assemblage for a wide range of cementitious binders, but does not provide information on the resulting pore structure. This work combined the Powers-Brownyard model, hydration kinetics, and thermodynamic calculations to predict capillary and gel porosity, chemical shrinkage, and volumes of all hydrated and unhydrated phases for plain cement pastes without the need for additional information such as pore size distributions. This new model has the advantages of both the Powers-Brownyard model and thermodynamic modeling, and is a powerful tool, which the authors envision to be used to further advance the understanding of durability of cementitious materials.
Disparities in HIV/AIDS Risk Behaviors After Youth Leave Detention: A 14-Year Longitudinal Study
Authors: Abram Karen M. , Stokes Marquita L. , Welty Leah J. , Aaby David A. , Teplin Linda A. 
Published: January 2017

OBJECTIVES:
To examine changes in the prevalence of 15 HIV/AIDS sex and drug risk behaviors in delinquent youth during the 14 years after they leave detention, focusing on sex and racial/ethnic differences.

METHODS:
The Northwestern Juvenile Project, a prospective longitudinal study of 1829 youth randomly sampled from detention in Chicago, Illinois, recruited between 1995 and 1998 and reinterviewed up to 11 times. Independent interviewers assessed HIV/AIDS risk behaviors using the National Institutes on Drug Abuse Risk Behavior Assessment.

RESULTS:
Fourteen years after detention (median age, 30 years), one-quarter of males and one-tenth of females had &gt;1 sexual partner in the past 3 months. One-tenth of participants reported recent unprotected vaginal sex with a high-risk partner. There were many sex and racial/ethnic differences. For example, African American males had 4.67 times the odds of having &gt;1 partner than African American females (95% confidence interval [CI], 3.22–6.76). Over time, compared with non-Hispanic white males, African American males had 2.56 times the odds (95% CI, 1.97–3.33) and Hispanic males had 1.63 times the odds (95% CI, 1.24–2.12) of having multiple partners, even after adjusting for incarceration and age. Non-Hispanic white females were more likely to have multiple partners than racial/ethnic minority females.

CONCLUSIONS:
Although rates decrease over time, prevalence of sex risk behaviors are much higher than the general population. Among males, racial/ethnic minorities were at particular risk. The challenge for pediatric health is to address how disproportionate confinement of racial/ethnic minority youth contributes to disparities in the HIV/AIDS epidemic.
E-cigarettes and National Adolescent Cigarette Use: 2004–2014
Authors: Dutra Lauren M. , Glantz Stanton A. 
Published: January 2017

BACKGROUND:
E-cigarette use is rapidly increasing among adolescents in the United States, with some suggesting that e-cigarettes are the cause of declining youth cigarette smoking. We hypothesized that the decline in youth smoking changed after e-cigarettes arrived on the US market in 2007.

METHODS:
Data were collected by using cross-sectional, nationally representative school-based samples of sixth- through 12th-graders from 2004–2014 National Youth Tobacco Surveys (samples ranged from 16 614 in 2013 to 25 324 in 2004). Analyses were conducted by using interrupted time series of ever (≥1 puff) and current (last 30 days) cigarette smoking. Logistic regression was used to identify psychosocial risk factors associated with cigarette smoking in the 2004–2009 samples; this model was then applied to estimate the probability of cigarette smoking among cigarette smokers and e-cigarette users in the 2011–2014 samples.

RESULTS:
Youth cigarette smoking decreased linearly between 2004 and 2014 (P = .009 for ever smoking and P = .05 for current smoking), with no significant change in this trend after 2009 (P = .57 and .23). Based on the psychosocial model of smoking, including demographic characteristics, willingness to wear clothing with a tobacco logo, living with a smoker, likelihood of smoking in the next year, likelihood of smoking cigarettes from a friend, and use of tobacco products other than cigarettes or e-cigarettes, the model categorized &lt;25% of current e-cigarette–only users (between 11.0% in 2012 and 23.1% in 2013) as current smokers.

CONCLUSIONS:
The introduction of e-cigarettes was not associated with a change in the linear decline in cigarette smoking among youth. E-cigarette–only users would be unlikely to have initiated tobacco product use with cigarettes.
Effectiveness of early quadriceps exercises after anterior cruciate ligament reconstruction
Authors: Pumell C 
Published: January 2017

Objective. To systematically review the published information regarding the effectiveness and safety of early postoperative quadriceps muscle exercise training on pain, joint laxity, function and range of motion in postoperative anterior cruciate ligament (ACL) reconstruction adult patients. Data sources. Five databases (CINAHL, PEDro, Pubmed, Science Direct and the Cochrane Library) were searched for studies published from January 1990 to May 2007. Study selection. Publications describing research into the effectiveness of early quadriceps exercises after ACL reconstruction were included. A total of three eligible articles met the inclusion criteria. Data extraction. A review of the three eligible studies was undertaken to describe the key study components. The PEDro Scale was used to determine the methodological quality of the selected trials and the level of evidence of all the eligible studies was categorised according to the evidence hierarchy by Lloyd-Smith.24 Relevant data were extracted by the two reviewer groups to reduce bias. Data synthesis. Due to study heterogeneity a meta-analysis could not be conducted. Effect sizes were calculated provided that sufficient data were provided. Outcome measures included range of motion (ROM), functional performance, pain and knee laxity. The methodological quality of the studies did not vary considerably across the studies and the average PEDro score was 66%. Marginal significant differences were noted in knee ROM at 1 month postoperatively, pain day 1 postoperatively, knee laxity and subjective evaluation of function at 6 months postoperatively. Conclusion. Early quadriceps exercises can be performed safely in the first 2 postoperative weeks, but clinically significant gains in ROM, function, pain and knee laxity were not evident. Further research should include standardised interventions, measurement time frames and outcome measurement tools to allow for a meta- analysis to be conducted
Antifungal actinomycetes associated with the pine bark beetle, Orthotomicus erosus, in South Africa
Authors: Human Zander R. , Slippers Bernard , De Beer Z. Wilhelm , Wingfield Michael J. , Venter Stephanus N. 
Published: January 2017

Actinomycete bacteria are often associated with insects that have a mutualistic association with fungi. These bacteria are believed to be important to this insect–fungus association as they produce antibiotics that exclude other saprophytic fungi from the immediate environment. The aim of this study was to investigate the presence of potentially protective actinomycetes associated with Orthotomicus erosus, an alien invasive pine bark beetle, in South Africa. This bark beetle and its relatives have an association with Ophiostomatales species which are often the only fungi found in the bark beetle galleries. We hypothesised that antibiotic-producing actinomycetes could be responsible for the paucity of other fungi in the galleries by producing compounds to which the Ophiostoma spp. are tolerant. Several actinomycetes in the genus Streptomyces and one Gordonia sp. were isolated from the beetle. Interestingly, most isolates were from the same species as actinomycetes associated with other pine-infesting insects from other parts of the world, including bark beetles and the woodwasp Sirex noctilio. Most actinomycetes isolated had strong antifungal properties against the selected test fungi, including Ophiostoma ips, which is the most common fungal symbiont of Orthotomicus erosus. Although the actinomycetes did not benefit Ophiostoma ips and the hypothesis was not supported, their sporadic association with Orthotomicus erosus suggests that they could have some impact on the composition of the fungal communities present in the bark beetle galleries, which is at present poorly understood.

Significance:

Discovery of four putative undescribed Streptomyces spp. with antibiotic potential
First record of the introduction of actinomycete bacteria with pine-infesting insects into South Africa
Actinomycetes from South Africa group with undescribed Streptomyces spp. from pine-infesting insects of North America