bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential alterations of resting‐state functional connectivity in generalized anxiety disorder and panic disorder
Authors: Cui Huiru , Zhang Jie , Liu Yicen , Li Qingwei , Li Hui , Zhang Lanlan , Hu Qiang , Cheng Wei , Luo Qiang , Li Jianqi , Li Wei , Wang Jijun , Feng Jianfeng , Li Chunbo , Northoff Georg 
Published: January 2016

AbstractGeneralized anxiety disorder (GAD) and panic disorder (PD) are most common anxiety disorders with high lifetime prevalence while the pathophysiology and disease‐specific alterations still remain largely unclear. Few studies have taken a whole‐brain perspective in the functional connectivity (FC) analysis of these two disorders in resting state. It limits the ability to identify regionally and psychopathologically specific network abnormalities with their subsequent use as diagnostic marker and novel treatment strategy. The whole brain FC using a novel FC metric was compared, that is, scaled correlation, which they demonstrated to be a reliable FC statistics, but have higher statistical power in two‐sample t‐test of whole brain FC analysis. About 21 GAD and 18 PD patients were compared with 22 matched control subjects during resting‐state, respectively. It was found that GAD patients demonstrated increased FC between hippocampus/parahippocampus and fusiform gyrus among the most significantly changed FC, while PD was mainly associated with greater FC between somatosensory cortex and thalamus. Besides such regional specificity, it was observed that psychopathological specificity in that the disrupted FC pattern in PD and GAD correlated with their respective symptom severity. The findings suggested that the increased FC between hippocampus/parahippocampus and fusiform gyrus in GAD were mainly associated with a fear generalization related neural circuit, while the greater FC between somatosensory cortex and thalamus in PD were more likely linked to interoceptive processing. Due to the observed regional and psychopathological specificity, their findings bear important clinical implications for the potential treatment strategy. Hum Brain Mapp 37:1459‐1473, 2016. © 2016 Wiley Periodicals, Inc.
Altered short‐term plasticity within the working memory neural network: Is it neuroticism or is it depression?
Authors: Bianchi Renzo , Laurent Eric 
Published: January 2016

AbstractIn the present article, we discuss (1) the importance of assessing and statistically considering both clinical and subclinical forms of depression when examining the relationship between neuroticism and short‐term plasticity within the working memory neural network, and (2) the hypothesis of an antagonism between neuroticism and conscientiousness in personality research. We suggest that (1) neuroticism and depression should be examined in a relational manner, and (2) neuroticism and conscientiousness should not be antagonized. Hum Brain Mapp 37:1512‐1513, 2016. © 2016 Wiley Periodicals, Inc.
Lithological control of land subsidence induced by groundwater withdrawal in new urban AREAS (Granada Basin, SE Spain). Multiband DInSAR monitoring
Authors: Notti Davide , Mateos Rosa María , Monserrat Oriol , Devanthéry Nùria , Peinado Tomás , Roldán Francisco Javier , Fernández‐Chacón Francisca , Galve Jorge Pedro , Lamas Francisco , Azañón Jose Miguel 
Published: January 2016

AbstractGround subsidence in the southeastern border of the Granada Basin (SE Spain) has been studied using remote sensing techniques. Over the last decades, the region has experienced a huge urban expansion, which has caused a substantial increase in water supply requirements. Water needs are exclusively met by groundwater by means of numerous pumping wells, which exploit a confined detrital aquifer of alluvial fan deposits with a heterogeneous facies distribution. A general piezometric level decline (up to 50 m) has been recorded in the aquifer during the past 30 years that has induced the generation of a subsiding area with oval shape oriented WNW‐ESE just where the new urban areas and pumping wells are located. Subsidence has been monitored by exploiting synthetic aperture radar (SAR) images from ENVISAT (2003–2009) and Cosmo‐SkyMed (2011–2014). A new approach, which combines A‐DInSAR and small‐area persistent scatterer interferometry (PSI) analysis, has been applied obtaining a good accuracy regarding temporal and spatial dimension of the subsidence. ENVISAT data (2003–2009) reveal subsidence rates up to 10–15 mm/year, and Cosmo‐SkyMed (2011–2014) values slightly lower; up to 10 mm/year. Temporal variations in the subsidence velocity are in accordance with the rainfall pattern and piezometric fluctuations in the aquifer. The sector with highest rates of subsidence does not correspond to the area with more intense groundwater exploitation but to the area with greater presence of clays in the confining layer of the aquifer. There is a clear lithological control in the spatial distribution of the ground subsidence. This work integrates detailed geological and hydrogeological data with differential SAR interferometry monitoring with the aim to better understand subsidence processes in detrital aquifers with small‐scale heterogeneity. Copyright © 2016 John Wiley & Sons, Ltd.
Winner of the Young Investigator Award of the Society for Biomaterials (USA) for 2016, 10th World Biomaterials Congress, May 17–22, 2016, Montreal QC, Canada: Aligned microribbon‐like hydrogels for guiding three‐dimensional smooth muscle tissue regeneration
Authors: Lee Soah , Tong Xinming , Han Li‐Hsin , Behn Anthony , Yang Fan 
Published: January 2016

AbstractSmooth muscle tissue is characterized by aligned structures, which is critical for its contractile functions. Smooth muscle injury is common and can be caused by various diseases and degenerative processes, and there remains a strong need to develop effective therapies for smooth muscle tissue regeneration with restored structures. To guide cell alignment, previously cells were cultured on 2D nano/microgrooved substrates, but such method is limited to fabricating 2D aligned cell sheets only. Alternatively, aligned electrospun nanofiber has been employed as 3D scaffold for cell alignment, but cells can only be seeded post fabrication, and nanoporosity of electrospun fiber meshes often leads to poor cell distribution. To overcome these limitations, we report aligned gelatin‐based microribbons (µRBs) as macroporous hydrogels for guiding smooth muscle alignment in 3D. We developed aligned µRB‐like hydrogels using wet spinning, which allows easy fabrication of tissue‐scale (cm) macroporous matrices with alignment cues and supports direct cell encapsulation. The macroporosity within µRB‐based hydrogels facilitated cell proliferation, new matrix deposition, and nutrient diffusion. In aligned µRB scaffold, smooth muscle cells showed high viability, rapid adhesion, and alignment following µRB direction. Aligned µRB scaffolds supported retention of smooth muscle contractile phenotype, and accelerated uniaxial deposition of new matrix (collagen I/IV) along the µRB. In contrast, cells encapsulated in conventional gelatin hydrogels remained round with matrix deposition limited to pericellular regions only. We envision such aligned µRB scaffold can be broadly applicable in growing other anisotropic tissues including tendon, nerves and blood vessel. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1064–1071, 2016.
Immunomodulatory effects of amniotic membrane matrix incorporated into collagen scaffolds
Authors: Hortensius Rebecca A. , Ebens Jill H. , Harley Brendan A. C. 
Published: January 2016

AbstractAdult tendon wound repair is characterized by the formation of disorganized collagen matrix which leads to decreases in mechanical properties and scar formation. Studies have linked this scar formation to the inflammatory phase of wound healing. Instructive biomaterials designed for tendon regeneration are often designed to provide both structural and cellular support. In order to facilitate regeneration, success may be found by tempering the body's inflammatory response. This work combines collagen‐glycosaminoglycan scaffolds, previously developed for tissue regeneration, with matrix materials (hyaluronic acid and amniotic membrane) that have been shown to promote healing and decreased scar formation in skin studies. The results presented show that scaffolds containing amniotic membrane matrix have significantly increased mechanical properties and that tendon cells within these scaffolds have increased metabolic activity even when the media is supplemented with the pro‐inflammatory cytokine interleukin‐1 beta. Collagen scaffolds containing hyaluronic acid or amniotic membrane also temper the expression of genes associated with the inflammatory response in normal tendon healing (TNF‐α, COLI, MMP‐3). These results suggest that alterations to scaffold composition, to include matrix known to decrease scar formation in vivo, can modify the inflammatory response in tenocytes. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1332–1342, 2016.
Biological functionality of extracellular matrix‐ornamented three‐dimensional printed hydroxyapatite scaffolds
Authors: Kumar A. , Nune K. C. , Misra R. D. K. 
Published: January 2016

AbstractThree‐dimensional (3D) printing is considered an ideally suitable method to fabricate patient specific implantable devices. The approach enabled to produce a porous scaffold with tailored physical, mechanical, and biological properties because of the flexibility to tune the scaffold architecture. The objective of the study described was to elucidate the determining role of cell‐laid extracellular matrix (ECM) in impacting biological response. In this regard, to mimic the natural ECM environment or the attributes of the native tissue, a natural ECM analogue surface was produced on the 3D printed and sintered hydroxyapatite (HA) scaffold surface by the mineralized ECM of the osteoblast. This involved the growth of osteoblast on 3D printed scaffolds, followed by differentiation to deposit the mineralized ECM on the biomaterial surface. The cells were removed from the mineralized matrix using freeze–thaw cycles to obtain a decellularized extracellular matrix (dECM) on the biomaterial surface. Subsequently, seeding of osteoblast on dECM‐ornamented HA scaffolds led to 3D growth with enhanced expression of prominent proteins, actin and vinculin. Based on preliminary observations of present study, it was underscored that HA scaffolds‐ornamented with dECM provided an optimized microenvironment conducive to the growth of 3D structural tissue and favorably promoted biological functionality because of the availability of an environment that promoted cell–cell and cell–scaffold interaction. The primary advantage of dECM is that it enabled constructive remodeling and promoted the formation of tissue in lieu of less functional tissue. The study opens‐up a new path for printing of 3D structures suitable to treat segmental bone defects. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1343–1351, 2016.
Proteomic and peptidomic profiling of Brazilian artisanal ‘Coalho’ cheese
Authors: Silva Roberto A , Bezerra Vilma S , Pimentel Maria do Carmo B , Porto Ana Lúcia F , Cavalcanti Maria Taciana H , Filho José Luiz L 
Published: January 2016

AbstractBACKGROUNDArtisanal ‘Coalho’ cheese is a product typically popular in the Brazilian north‐eastern region. Production of this cheese represents about 9.2% of the internal crude product of Pernambuco State. Several peptides are generated from hydrolysis of αS1‐, αS2‐, β‐, and κ‐caseins during manufacture of this cheese. The commercial importance of Brazilian artisanal ‘Coalho’ cheese justifies the examination of both the protein and peptide profiles of cheeses from six cities of the semi‐arid region of Pernambuco State, Brazil.RESULTSSDS‐PAGE of the aqueous extracts of ‘Coalho’ cheeses (WSP) showed bands of lactoferrin, β‐lactoglobulin, β‐lactoglobulin (dimer), α‐lactoalbumin, bovine serum albumin, α‐casein, β‐casein, κ‐casein and para‐κ‐casein. A total of 57 to 72 peptides were confirmed by mass spectra in the different samples of ‘Coalho’ cheese which 32 known peptides (11 from αS1‐casein, three from αS2‐casein, 15 from β‐casein and three from κ‐casein), comprising seven caseinphosphopeptides. Among the unidentified peptides, three showed high intensity peaks in all ‘Coalho’ cheeses studied (with molecular weights of 1597, 1725/1726, 2778/2779 Da).CONCLUSIONThe proteomic studies revealed peptides that may represent molecular markers or fingerprints for investigating the quality control and regional characterisation of these ‘Coalho’ cheeses. © 2016 Society of Chemical Industry
Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum
Authors: Castel Virginia , Zivanovic Svetlana , Jurat‐Fuentes Juan L , Santiago Liliana G , Rubiolo Amelia C , Carrara Carlos R , Harte Federico M 
Published: January 2016

AbstractBACKGROUNDBrea gum (BG) is an exudate from the Cercidium praecox tree that grows in semi‐arid regions of Argentina. Some previous studies on BG have shown physicochemical characteristics and functional features similar to those of gum arabic. However, there is a need to elucidate the molecular structure of BG to understand the functionality. In this sense, BG was fractionated using hydrophobic interaction chromatography and the obtained fractions were analyzed by size exclusion chromatography.RESULTSAnalysis of the fractions showed that the bulk of the gum (approx. 84% of the polysaccharides) was a polysaccharide of 2.79 × 103 kDa. The second major fraction (approx. 16% of the polysaccharides) was a polysaccharide–protein complex with a molecular mass of 1.92 × 105 kDa. A third fraction consisted of protein species with a wide range of molecular weights. The molecular weight distribution of the protein fraction was analyzed by size exclusion chromatography. Comparison of the elution profiles of the exudates in native and reducing conditions revealed that some of the proteins were forming aggregates through disulfide bridges in native conditions. Further analysis of the protein fraction by SDS‐PAGE showed proteins with molecular weight ranging from 6.5 to 66 kDa.CONCLUSIONSThe findings showed that BG consists of several fractions with heterogeneous chemical composition and polydisperse molecular weight distributions. © 2016 Society of Chemical Industry
PTSD Symptom Trajectories in Disaster Volunteers: The Role of Self‐Efficacy, Social Acknowledgement, and Tasks Carried Out
Authors: Thormar Sigridur B. , Sijbrandij Marit , Gersons Berthold P.R. , Van de Schoot Rens , Juen Barbara , Karlsson Thorlakur , Olff Miranda 
Published: January 2016

AbstractMillions of volunteers respond after disasters, with a 24% to 46% risk of developing posttraumatic stress disorder (PTSD). It is unclear which symptom trajectories develop and how they differ between core (volunteering before the disaster) and noncore volunteers (joining after the disaster) and which factors predict trajectories. Symptoms of PTSD were assessed at 6‐, 12‐, and 18‐months postearthquake in 449 volunteers in Indonesia. Demographics, previous mental health service use, self‐efficacy, social acknowledgment, and type of tasks were assessed at 6 months. In both core and noncore volunteers, 2 PTSD symptom trajectories emerged: a resilient trajectory (moderate levels of symptoms with a slow decrease over time; 90.9%) and a chronic trajectory (higher levels of symptoms with an increase over time; 9.1%). In both trajectories, core volunteers had fewer symptoms than noncore volunteers. Core volunteers in the chronic trajectory were characterized by having sought prior mental help, reported lower levels of self‐efficacy and social acknowledgment, and were more likely to have provided psychosocial support to beneficiaries (Cramér's V = .17 to .27, partial η2 = .02 to .06). Aid organizations should identify and follow up chronic PTSD trajectories in volunteers, including the noncore, who may be out of sight to the organization after the acute response phase.
Chromosome loci vary by juvenile myoclonic epilepsy subsyndromes: linkage and haplotype analysis applied to epilepsy and EEG 3.5–6.0 Hz polyspike waves
Authors: Wight Jenny E. , Nguyen Viet‐Huong , Medina Marco T. , Patterson Christopher , Durón Reyna M. , Molina Yolly , Lin Yu‐Chen , Martínez‐Juárez Iris E. , Ochoa Adriana , Jara‐Prado Aurelio , Tanaka Miyabi , Bai Dongsheng , Aftab Sumaya , Bailey Julia N. , Delgado‐Escueta Antonio V. 
Published: January 2016

AbstractJuvenile myoclonic epilepsy (JME), the most common genetic epilepsy, remains enigmatic because it is considered one disease instead of several diseases. We ascertained three large multigenerational/multiplex JME pedigrees from Honduras with differing JME subsyndromes, including Childhood Absence Epilepsy evolving to JME (CAE/JME; pedigree 1), JME with adolescent onset pyknoleptic absence (JME/pA; pedigree 2), and classic JME (cJME; pedigree 3). All phenotypes were validated, including symptomatic persons with various epilepsies, asymptomatic persons with EEG 3.5–6.0 Hz polyspike waves, and asymptomatic persons with normal EEGs. Two‐point parametric linkage analyses were performed with 5185 single‐nucleotide polymorphisms on individual pedigrees and pooled pedigrees using four diagnostic models based on epilepsy/EEG diagnoses. Haplotype analyses of the entire genome were also performed for each individual. In pedigree 1, haplotyping identified a 34 cM region in 2q21.2–q31.1 cosegregating with all affected members, an area close to 2q14.3 identified by linkage (Zmax = 1.77; pedigree 1). In pedigree 2, linkage and haplotyping identified a 44 cM cosegregating region in 13q13.3–q31.2 (Zmax = 3.50 at 13q31.1; pooled pedigrees). In pedigree 3, haplotyping identified a 6 cM cosegregating region in 17q12. Possible cosegregation was also identified in 13q14.2 and 1q32 in pedigree 3, although this could not be definitively confirmed due to the presence of uninformative markers in key individuals. Differing chromosome regions identified in specific JME subsyndromes may contain separate JME disease‐causing genes, favoring the concept of JME as several distinct diseases. Whole‐exome sequencing will likely identify a CAE/JME gene in 2q21.2–2q31.1, a JME/pA gene in 13q13.3–q31.2, and a cJME gene in 17q12.
Recent bioinspired applications of single‐chain nanoparticles
Authors: Latorre‐Sánchez Alejandro , Pomposo José A 
Published: January 2016

AbstractSingle‐chain nanoparticles (SCNPs) are polymeric soft nano‐objects constructed via individual chain compaction which are envisioned as versatile building blocks for the construction of future smart, biofunctional nanodevices. According to their morphology in solution, SCNPs can be classified as (i) sparse SCNPs resembling the morphology of intrinsically disordered proteins having local compact zones connected by flexible spacers and (ii) globular SCNPs having a morphology more akin to that found in native, compact proteins (i.e. enzymes). The objective of this mini‐review is to highlight recent bioinspired applications of SCNPs and to give an insight into the current trends of this rapidly evolving field. After a brief introduction to the main techniques employed to construct SCNPs and a clear description of how to tune the morphology in solution of SCNPs, very recent examples of the use of SCNPs as binding protein mimics, reductase‐, oxidase‐, aldolase‐, polymerase‐ enzyme and metalloenzyme mimics, as well as efficient biosensing elements are described. © 2016 Society of Chemical Industry
NlpC/P60 domain‐containing proteins of Mycobacterium avium subspecies paratuberculosis that differentially bind and hydrolyze peptidoglycan
Authors: Bannantine John P. , Lingle Cari K. , Adam Philip R. , Ramyar Kasra X. , McWhorter William J. , Stabel Judith R. , Picking William D. , Geisbrecht Brian V. 
Published: January 2016

AbstractA subset of proteins containing NlpC/P60 domains are bacterial peptidoglycan hydrolases that cleave noncanonical peptide linkages and contribute to cell wall remodeling as well as cell separation during late stages of division. Some of these proteins have been shown to cleave peptidoglycan in Mycobacterium tuberculosis and play a role in Mycobacterium marinum virulence of zebra fish; however, there are still significant knowledge gaps concerning the molecular function of these proteins in Mycobacterium avium subspecies paratuberculosis (MAP). The MAP genome sequence encodes five NlpC/P60 domain‐containing proteins. We describe atomic resolution crystal structures of two such MAP proteins, MAP_1272c and MAP_1204. These crystal structures, combined with functional assays to measure peptidoglycan cleavage activity, led to the observation that MAP_1272c does not have a functional catalytic core for peptidoglycan hydrolysis. Furthermore, the structure and sequence of MAP_1272c demonstrate that the catalytic residues normally required for hydrolysis are absent, and the protein does not bind peptidoglycan as efficiently as MAP_1204. While the NlpC/P60 catalytic triad is present in MAP_1204, changing the catalytic cysteine‐155 residue to a serine significantly diminished catalytic activity, but did not affect binding to peptidoglycan. Collectively, these findings suggest a broader functional repertoire for NlpC/P60 domain‐containing proteins than simply hydrolases.
Use of noise in gene expression as an experimental parameter to test phenotypic effects
Authors: Liu Jian , François Jean‐Marie , Capp Jean‐Pascal 
Published: January 2016

AbstractDuring the last decade, the molecular basis for gene expression noise has been mostly deciphered, helping understanding of how gene regulation is controlled and how the generation of cell–cell non‐genetic heterogeneity is modulated through noise. In the same period, the functional importance of phenotypic heterogeneity among cell populations has been recognized and widely involved in major biological phenomena. Surprisingly, only a few studies connect these two highly active research fields, most of them having been obtained using the yeast Saccharomyces cerevisiae. This organism has long been the preferred model for studying many aspects of gene expression noise, especially revealing that evolution seems to act to either increase or decrease gene expression noise, depending on whether the associated phenotypic heterogeneity is beneficial or deleterious to the population. Nevertheless, direct evidences of phenotypic consequences of noise differences are often lacking, in spite of this evolutionary tendency. This rarity is probably due to the complex relationships between mean and noise levels, making the study of the sole effect of noise difficult, and also to problems caused by the detection of cell–cell expression variability of native functional proteins, allowing the testing of specific phenotypic effects. Despite these difficulties, the widespread use of gene expression noise as an experimental parameter at equal mean expression levels to test phenotypic consequences would often help to change explanations of cell population behaviour beyond the simple consideration of average expression levels, and constitute a major step towards single‐cell biology. Copyright © 2016 John Wiley & Sons, Ltd.