bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired Central Pulsatile Hemodynamics in Children and Adolescents With Marfan Syndrome
Authors: Grillo Andrea , Salvi Paolo , Marelli Susan , Gao Lan , Salvi Lucia , Faini Andrea , Trifirò Giuliana , Carretta Renzo , Pini Alessandro , Parati Gianfranco 
Published: November 2017

Background
Marfan syndrome is characterized by aortic root dilation, beginning in childhood. Data about aortic pulsatile hemodynamics and stiffness in pediatric age are currently lacking.

Methods and Results

In 51 young patients with Marfan syndrome (12.0±3.3 years), carotid tonometry was performed for the measurement of central pulse pressure, pulse pressure amplification, and aortic stiffness (carotid‐femoral pulse wave velocity). Patients underwent an echocardiogram at baseline and at 1 year follow‐up and a genetic evaluation. Pathogenetic fibrillin‐1 mutations were classified between “dominant negative” and “haploinsufficient.” The hemodynamic parameters of patients were compared with those of 80 sex, age, blood pressure, and heart‐rate matched controls. Central pulse pressure was significantly higher (38.3±12.3 versus 33.6±7.8 mm Hg;
P
=0.009), and pulse pressure amplification was significantly reduced in Marfan than controls (17.9±15.3% versus 32.3±17.4%;
P
<0.0001). Pulse wave velocity was not significantly different between Marfan and controls (4.98±1.00 versus 4.75±0.67 m/s). In the Marfan group, central pulse pressure and pulse pressure amplification were independently associated with aortic diameter at the sinuses of Valsalva (respectively, β=0.371,
P
=0.010; β=−0.271,
P
=0.026). No significant difference in hemodynamic parameters was found according to fibrillin‐1 genotype. Patients who increased aortic Z‐scores at 1‐year follow‐up presented a higher central pulse pressure than the remaining (42.7±14.2 versus 32.3±5.9 mm Hg;
P
=0.004).

Conclusions
Central pulse pressure and pulse pressure amplification were impaired in pediatric Marfan syndrome, and associated with aortic root diameters, whereas aortic pulse wave velocity was similar to that of a general pediatric population. An increased central pulse pressure was present among patients whose aortic dilatation worsened at 1‐year follow‐up.
Quantifying the Rain-Shadow Effect: Results from the Peak District, British Isles
Authors: Stockham Alexander J. , Schultz David M. , Fairman Jonathan G. , Draude Adam P. 
Published: November 2017

AbstractAlthough rain shadows (i.e., leeside reductions of precipitation downwind of orography) are commonly described in textbooks, quantitative climatologies of the rain-shadow effect are rare. To test quantitatively a classic rain-shadow locality of the Peak District, United Kingdom, precipitation from 54 observing stations over 30 years (1981–2010) are examined. Under 850-hPa westerlies, annual and daily precipitation amounts are on average higher in Manchester in the west and the Peak District than in Sheffield in the east. More precipitation falls—and falls more frequently—frequently in Manchester than Sheffield on 197 westerly flow days annually. In contrast, more precipitation falls—and falls more frequently—in Sheffield than Manchester on 28 easterly flow days annually. These bulk precipitation statistics support a climatological rain shadow. However, when individual days are investigated, only 17% of westerly flow days occur where daily rainfall data might exhibit the rain-shadow effect (defined here as Manchester with precipitation and Sheffield with no precipitation). In contrast, only 10% of easterly flow days occur where daily rainfall data might exhibit the rain-shadow effect (Sheffield with precipitation and Manchester with no precipitation). Thus, westerly winds are more likely to exhibit a rain-shadow effect than easterly winds. Although the distribution of precipitation observed across the Peak District can sometimes be explained by the rain-shadow effect, the occurrence of the rain-shadow effect by our admittedly strict definition is not as frequent as one might expect to explain the local precipitation climate for which it has sometimes been previously credited. Thus, an attempt to understand the climatological relevance of the rain-shadow effect from one location reveals ambiguity in the definition of a rain shadow and in its interpretation from real rainfall data.
Variations of Climate, Surface Energy Budget, and Minimum Snow/Ice Extent over Canadian Arctic Landmass for 2000–16
Authors: Trishchenko Alexander P. , Wang Shusen 
Published: November 2017

Abstract
Snow and ice over land are important hydrological resources and sensitive indicators of climate change. The Moderate Resolution Imaging Spectroradiometer (MODIS) dataset at 250-m spatial resolution generated at the Canada Centre for Remote Sensing (CCRS) is used to derive the annual minimum snow and ice (MSI) extent over the Canadian Arctic landmass over a 17-yr time span (2000–16). The smallest MSI extent (1.53 × 105 km2) was observed in 2012, the largest (2.09 × 105 km2) was observed in 2013; the average value was 1.70 × 105 km2. Several reanalyses and observational datasets are assessed to explain the derived MSI variations: the ERA-Interim reanalysis, North American Regional Reanalysis (NARR), Clouds and the Earth’s Radiant Energy System (CERES) radiative fluxes, and European Space Agency’s GlobSnow dataset. Comparison with the Randolph Glacier Inventory (RGI) showed two important facts: 1) the semipermanent snowpack in the Canadian Arctic that persists through the entire melting season is a significant component relative to the ice caps and glacier-covered areas (up to 36% or 5.58 × 104 km2), and 2) the MSI variations are related to variations in the local climate dynamics such as warm season average temperature, energy fluxes, and snow cover. The correlation coefficients (absolute values) can be as high as 0.77. The reanalysis-based MSI estimates agree with satellite MSI results (average bias of 2.2 × 103 km2 or 1.3% of the mean value).
A Probabilistic Tropical Cyclone Track Forecast Scheme Based on the Selective Consensus of Ensemble Prediction Systems
Authors: Zhang Xiping , Yu Hui 
Published: November 2017

Abstract
Selective consensus and a grand ensemble based on an ensemble prediction system (EPS) have been found to be effective in improving deterministic tropical cyclone (TC) track forecasts, while little attention has been paid to quantitative applications of the forecast uncertainty information provided by EPSs. In this paper the forecast uncertainty information is evaluated for two operational EPSs and their grand ensemble. Then, a probabilistic TC track forecast scheme is proposed based on the selective consensus of the two EPSs; this scheme is composed of member picking, mean track shifting, and probability ellipses. The operational EPSs are from the European Centre for Medium-Range Weather Forecasts (ECMWF-EPS) and the National Centers for Environmental Prediction (NCEP-GEFS). Evaluation exhibits that the hit ratios of ECMWF-EPS are above 80% for the 70% probability ellipses at all lead times until 120 h and are used in the proposed scheme. The other components of the proposed scheme are about picking potentially good EPS members. A picking ratio of 1/2 is found to be the best choice, and the member-picking technique is used for the grand ensemble but only for lead times out to 48 h. For lead times longer than 48 h, all of the grand ensemble members are used in obtaining the mean track. The effectiveness of the proposed scheme shows a 10% improvement in the mean track forecast errors over the grand ensemble and a 4.5% improvement in the hit ratio of 70% probability ellipses over the ECMWF-EPS at 24 h, demonstrating its good potential to be applied in operations.
UK-trained junior doctors' intentions to work in UK medicine: questionnaire surveys, three years after graduation
Authors: Surman Geraldine , Goldacre Michael J , Lambert Trevor W 
Published: November 2017

Objective To report on the career intentions, three years after qualification, of 12 national cohorts of UK-trained doctors who qualified between 1974 and 2012, and, specifically, to compare recent UK medical graduates’ intentions to work in medicine in the UK with earlier graduates. Design Questionnaire surveys of cohorts of UK medical graduates defined by year of graduation. Setting UK. Participants 30,272 UK medical graduates. Main outcome measures Stated level of intention to pursue a long-term career in medicine in the UK. Results The response rate was 62% (30,272/48,927). We examined responses to the question ‘ Apart from temporary visits abroad, do you intend to practise medicine in the United Kingdom for the foreseeable future?' Of doctors from UK homes, 90% had specified that they would ‘definitely or probably’ practise medicine in the UK in the surveys of 1977–1986, 81% in 1996–2011 and 64% in 2015. Those who said that they would probably or definitely not practise medicine in the UK comprised 5% in 1977–1986, 8% in 1996–2011 and 15% in 2015. Most who were not definite about a future career in UK medicine indicated that they would wish to practise medicine outside the UK rather than to leave medicine. Conclusions The wish to remain in UK medical practice in the 2015 survey was unprecedentedly low in this unique series of 40 years of surveys.
Hypothermia Promotes Cell-Protective and Chondroprotective Effects After Blunt Cartilage Trauma
Authors: Riegger Jana , Zimmermann Madeleine , Joos Helga , Kappe Thomas , Brenner Rolf E. 
Published: November 2017

Background: Cryotherapy is routinely administered after sports injuries of synovial joints. Although positive clinical effects on periarticular swelling and pain have been described, the effects on the cell biological activities of cartilage and synovial cells remain largely unknown so far. Hypothesis: Local hypothermia alleviates synovial reactions and prevents chondrocyte death as well as cartilage destructive processes after blunt cartilage trauma. Study Design: Controlled laboratory study. Methods: Human cartilage explants were impacted by a drop-tower apparatus (0.59 J) and cultured at 24 hours or 7 days in different temperature conditions (2 hours [short term], 16 hours [medium term], or throughout [long term] at 27°C; afterwards or throughout at 37°C). Besides, isolated human fibroblast-like synoviocytes (FLS) were stimulated with traumatized cartilage conditioned medium and cultured as mentioned above up to 4 days. The effects of hypothermia were evaluated by cell viability, gene expression, type II collagen synthesis and cleavage, as well as the release of matrix metalloproteinase (MMP)–2, MMP-13, and interleukin 6 (IL-6). Results: Seven days after trauma, hypothermic treatment throughout improved cell viability (short term: 10.1% [ P = .016]; medium term: 6% [ P = .0362]; long term: 12.5% [ P = .0039]). Short-term hypothermia attenuated the expression of catabolic MMP-13 (mRNA: –2.2-fold [ P = .0119]; protein: –2-fold [ P = .0238]). Whereas type II collagen synthesis (1.7-fold [ P = .0227]) was increased after medium-term hypothermia, MMP-13 expression (mRNA: –30.8-fold [ P = .0025]; protein: –10.3-fold [ P < .0001]) and subsequent cleavage of type II collagen (–1.1-fold [ P = .0489]) were inhibited. Long-term hypothermia further suppressed MMP release (pro–MMP-2: –3-fold [ P = .0222]; active MMP-2: −5.2-fold [ P = .0183]; MMP-13: −56-fold [ P < .0001]) and type II collagen breakdown (–1.6-fold [ P = .0036]). Four days after FLS stimulation, hypothermia significantly suppressed the gene expression of matrix-destructive enzymes after medium-term (MMP-3: –4.1-fold [ P = .0211]) and long-term exposure (a disintegrin and metalloproteinase with thrombospondin motifs 4 [ADAMTS4]: –4.3-fold [ P = .0045]; MMP-3: –25.8-fold [ P = .014]; MMP-13: –122-fold [ P = .0444]) and attenuated IL-6 expression by trend. Conclusion: After blunt cartilage trauma, initial hypothermia for only 2 hours and/or 16 hours induced significant cell-protective and chondroprotective effects and promoted the anabolic activity of chondrocytes, while the expression of matrix-destructive enzymes by stimulated FLS was attenuated by prolonged hypothermia. Clinical Relevance: The findings of this preliminary ex vivo investigation indicate that optimized cryotherapy management after cartilage trauma might prevent matrix-degenerative processes associated with the pathogenesis of posttraumatic osteoarthritis.
Enhanced cytocompatibility and osteoinductive properties of sol–gel-derived silica/zirconium dioxide coatings by metformin functionalization
Authors: Śmieszek Agnieszka , Szydlarska Joanna , Mucha Aleksandra , Chrapiec Martyna , Marycz Krzysztof 
Published: November 2017

The aim of this study was to evaluate the pro-osteogenic properties of sol–gel-derived silica/zirconium dioxide coatings functionalized with 1 mM of metformin. The matrices were applied on 316L stainless steel using dip-coating technique. First of all, physicochemical properties of biomaterials were evaluated. Surface morphology and topography was determined using energy-dispersive X-ray spectroscopy and atomic force microscopy. The chemical composition was evaluated using Fourier transform infrared spectroscopy. Further, wettability and surface free energy were characterized. Cytocompatibility of biomaterials was tested in vitro using model of human multipotent mesenchymal stromal cells isolated from adipose tissue. The influence of biomaterials on cells morphology and proliferation was determined. Osteogenic effect of obtained biomaterials was evaluated in terms of their influence on secretory activity of human multipotent mesenchymal stromal cells isolated from adipose tissue and matrix mineralization. Analysis was performed in relation to the control cultures i.e. maintained on pure SS316L substrate and SS316L covered with silica/zirconium dioxide. Obtained results indicate that silica/zirconium dioxide_metformin coatings ameliorated metabolic and proliferative activity of human multipotent mesenchymal stromal cells isolated from adipose tissue, as well as promoted their proper growth and adhesion. The human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on biomaterials were characterized by typical fibroblast-like morphology. The addition of metformin to the silica/zirconium dioxide coatings improved functional differentiation of human multipotent mesenchymal stromal cells isolated from adipose tissue. Osteogenic cultures on silica/zirconium dioxide_metformin were characterized by formation of well-developed osteonodules rich in calcium and phosphorous. Moreover, human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on silica/zirconium dioxide_metformin synthesized increased amount of alkaline phosphatase, bone morphogenetic protein 2 and osteopontin, both on messenger RNA and protein level. Obtained biomaterials modulate cellular plasticity of human multipotent mesenchymal stromal cells isolated from adipose tissue promoting their osteogenic differentiation, thus may find application in broadly defined tissue engineering.
Rapid in situ cross-linking of hydrogel adhesives based on thiol-grafted bio-inspired catechol-conjugated chitosan
Authors: Zeng Zhiwen , Mo Xiumei 
Published: November 2017

In this paper, a novel chitosan derivative, thiol-grafting bio-inspired catechol-conjugated chitosan was synthesized. The chemical structure of the synthesized catechol-conjugated chitosan was verified by 1H NMR, and its contents of thiol group and catechol group were determined by UV-vis spectrum. Four percent of catechol-conjugated chitosan aqueous solution could form hydrogels rapidly in situ in 1 min or less with the addition of sodium periodate. Rheological studies showed that the mechanical properties depend on the concentrations of catechol-conjugated chitosan and the molar ratio of sodium periodate to catechol groups. Additionally, the adhesive properties of the resulting adhesives were evaluated, and the adhesion strength of obtained adhesives was as high as 50 kPa because of the complex and multiple interactions, especially the anti-oxidation mechanism of thiol group. The in vitro cytotoxicity assays demonstrated an excellent biocompatibility of the catechol-conjugated chitosan hydrogels. Benefiting from the in situ fast cured, desired mechanical strength, biocompatibility and relatively high adhesion performance, these properties suggested that catechol-conjugated chitosan hydrogel adhesives have potential applications as tissue adhesive for soft tissues.
Evaluation of Cellular Fibronectin Plasma Levels as a Useful Staging Tool in Different Stages of Transitional Cell Carcinoma of the Bladder and Renal Cell Carcinoma
Authors: Hegele A. , Hofmann R. , Kosche B. , Kropf J. 
Published: November 2017

Reliable markers for both renal cell carcinoma (RCC) and transitional cell carcinoma of the bladder (TCC) are lacking.During tumor progression and invasion components of extracellular matrix (ECM) are degraded and parts of these different components are detectable in plasma. Cellular fibronectin (cFN) represents a well characterized ECM protein. In contrast to fibronectin in plasma produced by hepatocytes (FN) cFN has a total extra domain sequence and occurs in much smaller amounts in the circulation. The aim of our study was to evaluate cFN as a marker and to determine its possible role in clinical staging of TCC and RCC.Blood samples were collected from 30 patients before they underwent transurethral resection of the bladder because of newly diagnosed TCC. Additionally samples were collected from 69 patients with RCC before therapy. Sixty patients with non-malignant urological disorders were recruited as control group. Determination of cFN in plasma was performed by using a highly sensitive time-resolved fluorescence immunoassay (TRFIA).The control group had median cFN plasma levels of 437 ng/ml. Patients suffering from TCC or RCC showed significantly higher cFN levels. In patients with muscle invasive TCC significant higher cFN levels (p < 0.05) could be demonstrated compared to non-muscle invasive TCC. Similar results were found in RCC with significant elevated cFN levels in metastatic RCC (p < 0.005) compared to localized stage of disease. No differences were found concerning tumor grading in both malignancies.In the face of significant elevated cFN levels in TCC and RCC our data underline the important role of cFN. For future investigations the elevated cFN levels in locally progressed and metastastic disease, indicating a clinically useful tool for preoperative staging and postoperative monitoring, are of high interest.
Genetic Alterations in Pesticide Exposed Bolivian Farmers An evaluation by analysis of chromosomal aberrations and the comet assay
Authors: Jørs Erik , Gonzáles Ana Rosa , Ascarrunz Maria Eugenia , Tirado Noemi , Takahashi Catharina , Lafuente Erika , Santos Raquel A Dos , Bailon Natalia , Cervantes Rafael , Huici O , Bælum Jesper , Lander Flemming 
Published: November 2017

Background Pesticides are of concern in Bolivia because of increasing use. Frequent intoxications have been demonstrated due to use of very toxic pesticides, insufficient control of distribution and sale and little knowledge among farmers of protective measures and hygienic procedures. Method Questionnaires were applied and blood tests taken from 81 volunteers from La Paz County, of whom 48 were pesticide exposed farmers and 33 non-exposed controls. Sixty males and 21 females participated with a mean age of 37.3 years (range 17–76). Data of exposure and possible genetic damage were collected and evaluated by well known statistical methods, controlling for relevant confounders. To measure genetic damage chromosomal aberrations and the comet assay analysis were performed. Results Pesticide exposed farmers had a higher degree of genetic damage compared to the control group. The number of chromosomal aberrations increased with the intensity of pesticide exposure. Females had a lower number of chromosomal aberrations than males, and people living at altitudes above 2500 metres seemed to exhibit more DNA damage measured by the comet assay. Conclusions Bolivian farmers showed signs of genotoxic damage, probably related to exposure to pesticides. Due to the potentially negative long term health effects of genetic damage on reproduction and the development of cancer, preventive measures are recommended. Effective control with imports and sales, banning of the most toxic pesticides, education and information are possible measures, which could help preventing the negative effects of pesticides on human health and the environment.
Studies of Wilms’ Tumor (WT1) Gene Expression in Adult Acute Leukemias in Singapore
Authors: Lim Che Kang , Goh Yeow Tee , Hwang William Y. K. , Ho Liam Pock , Sun Li 
Published: November 2017

Biomarkers provide certain values for diagnosis, monitor treatment efficacy, or for the development of novel therapeutic approach for particular diseases. Thus, the identification of specific of biomarkers for specific medical problems, including malignant diseases may be valuable in medical practice. In the study, we have used the Wilms’ tumor gene ( WT1) as a biomarker to evaluate its expression in local adult patients with newly diagnosed acute leukemia, including both acute myeloid and lymphoid leukemias (AML and ALL). Aim To investigate WT1 gene expression in adult patients with acute leukemia at diagnosis. Methods Eighteen patients with acute leukemia diagnosed at Singapore General Hospital, Singapore, between September, 2004 and July, 2005 were included in this study. There were fifteen AML and three ALL cases aged from 18 to 71 years old. Total RNA and DNA was extracted from peripheral blood mononuclear cells (PBMCs). Expression of WT1 was detected by nested reverse-transcription polymerase chain reaction (Nested RT-PCR). K562, and 3T3 cells were used as positive- and negative-controls. The results were revalidated using real-time PCR. HLA-A genotyping was performed using sequence specific oligonucleotide polymorphism (SSOP) analysis. Results WT1 gene was exclusively expressed in all eighteen, including three ALL and fifteen AML, patients. In contrast with WT1 gene, the HLA-A genotyping was remarkably heterogeneous in these patients. Conclusions WT1 gene expression was observed in local patients with acute leukemia at diagnosis. It may be used as a potential molecular marker for diagnosis, clinical progression of the diseases or monitoring the response to treatment, as well as a target for the development of novel therapeutic approaches.