bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analytical and Diagnostic Performance of a Dual‐Target Blood Detection Test for Hepatocellular Carcinoma
Authors: Yang Qiankun , Dong Lanlan , Zhang Lianglu , Zhang Wei , Zhang Yan , Huang Yue , Jin Huifang , Yang Hao , Liu Xing , Zhao Yanteng 
Published: September 2024

ABSTRACTBackgroundSurveillance approaches with high sensitivity and specificity for hepatocellular carcinoma (HCC) are still urgently needed. Previous studies have shown that methylation of GNB4 and Riplet can effectively diagnose HCC.AimsThis study plan to analyze the performance of a blood test for detecting HCC using GNB4 and Riplet methylation.Methods and ResultsThis study mainly investigated the analytical performance of the dual‐target HCC blood test (DT‐HBT), including cut‐off value, limit of detection (LOD), precision, analytical specificity, and coincidence rate. In addition, the detection performance for HCC was validated in 1030 clinical plasma samples (214 HCC and 816 non‐HCC). Plasma samples from 25 HCC patients after hepatectomy were collected to assess the feasibility of the kit for postoperative recurrence monitoring. All analytical performance of the DT‐HBT met prespecified requirements. The LOD for GNB4, Riplet, and β‐actin was 1% methylation/100 copies/μL with cut‐offs of 43, 43, and 35, respectively. The DT‐HBT showed excellent precision, within 5% CV. It had a specificity of 91.5% for detecting other cancers, and 100% for breast, lung, and bladder cancer. No cross‐reactions were observed with 9 potential interfering substances. The DT‐HBT achieved a 100% coincidence rate in detecting reference and clinical samples. The clinical performance study found that the kit showed a sensitivity of 81.7% for stage I HCC, and an overall sensitivity and specificity of 87.4% and 92.3%, respectively. The detection sensitivity for postoperative recurrent patients was 95.8%, with a specificity of 100%.ConclusionThe analytical performance of the DT‐HBT met prespecified criteria. It provided HCC patients with a reliable and high‐performing new blood test for the HCC diagnosis and surveillance.Trial RegistrationClinicalTrials.govidentifier: NCT05685524
Complex epilepsy phenotype associated with chromosome 2q24.2‐q24.3 deletion involving sodium channel gene cluster
Authors: Madan Rima , Guido Fiorella S. , Brescia Nicole 
Published: September 2024

AbstractObjectiveThe 2q24.2‐24.3 chromosome region encodes sodium channel genes important in severe childhood epilepsy, notably SCN1A linked to Dravet syndrome (DS). However, the roles of other genes, either within the SCN cluster or in the segments proximal to it, have not been clearly delineated. The combination of ketogenic diet and fenfluramine is known to provide substantial benefits to patients with DS, but there is a paucity of literature regarding its role in other developmental and epileptic encephalopathies (DEE). This report aims to further explore clinical findings and treatment outcomes in a patient with a complex epilepsy phenotype.MethodsOur patient's extensive diagnostic evaluation revealed 14 deleted genes within the 2q24.2‐24.3 chromosome region.ResultsThe patient initially presented with clusters of focal motor seizures with apnea and cyanosis requiring intubation as well as prolonged hospitalizations for status epilepticus. He has baseline hypotonia, dysphagia, and developmental delay. He had a >50% reduction in his seizures following a combination of ketogenic diet and fenfluramine. His seizures are now responsive to rescue midazolam, and he no longer has status epilepticus.InterpretationOur patient's remarkable clinical improvement suggests that this dual therapy may be beneficial in patients with DEE exhibiting pathogenic variations in this region of chromosome 2, beyond just DS.
A Sonochemical and Mechanochemical One‐Pot Multicomponent/Click Coupling Strategy for the Sustainable Synthesis of Bis‐Heterocyclic Drug Scaffolds
Authors: Rentería‐Gómez Manuel A. , Calderón‐Rangel David , Corona‐Díaz Alejandro , Gámez‐Montaño Rocío 
Published: September 2024

AbstractBis‐heterocycles were synthesized via a consecutive one‐pot process by a Groebke‐Blackburn‐Bienaymé reaction (GBB‐3CR) followed by Copper‐catalyzed Alkyne‐Azide Cycloaddition (CuAAC) assisted by alternative sustainable energies (ASE) such as ultrasonic and mechanical. These efficient and convergent strategies allowed the in situ generation of complex azides functionalized with imidazo[1,2‐a]pyridines (IMPs), which was used as a synthetic platform. The target molecules contain two privileged scaffolds in medicinal chemistry: IMPs and the heterocyclic bioisostere of trans‐amide bond, the 1,4‐disubstituted 1H‐1,2,3‐triazoles (1,4‐DS‐1,2,3‐Ts).
Deciphering the Degradation Mechanism Induced by the Air‐Exposure in Li2NiO2 and the Recovery Strategy
Authors: Li Shirui , Xiao Kuikui , Liu Xi , Sun Jiangdong , Cai Dong , Yang Shuo , Zhang Yinhang , Nie Huagui , Yang Zhi 
Published: September 2024

AbstractCathode prelithium agent is regarded as the most applicable approach to compensate the initial capacity loss in lithium ion batteries (LIBs). Li2NiO2 (LNO) has attracted numerous attention due to its superior environmental stability and reliable synthesis approach. To promote the commercial application of LNO, the understanding of the degradation mechanism induced by air‐exposure and finding reliable strategies to improve the air stability are necessary. Herein, by preserving the LNO in different environments (relative humidity of 70 % and 40 %), the surface chemistry evolution of LNO is subtly investigated, which shows Li2CO3 and LiOH cover the surface of the LNO, which decline the Li+ diffusion kinetics as well as the charge capacity. What′s more, the slurry turns gel when the LNO exposed to the environment of 70 % relative humidity for 2 days and 40 % relative humidity for 5 days. Facile approaches, including washing the deteriorative LNO with ethanol, reacting the alkali components with H3BO3, and coating the LNO with Al2O3 are conducted to recover the disabled LNO, which retains 84.2 % of initial capacity. In addition, a coating approach is proposed for the fresh LNO to effectively improve the air stability. This work provides guideline to the commercial application of the LNO.
Terahertz Wave Absorption of a Rubidium Manganese‐Iron Prussian Blue Phase Transition Material
Authors: Mineo Yuuki , Nakabayashi Koji , Tokoro Hiroko , Imoto Kenta , Namai Asuka , Yoshikiyo Marie , Ohkoshi Shin‐ichi 
Published: September 2024

AbstractRubidium manganese hexacyanidoferrate, Rb0.97Mn[Fe(CN)6]0.99 ⋅ 0.5H2O, which has a three‐dimensional cyanido‐bridged Mn−Fe framework encapsulating Rb+ ions, shows a terahertz (THz) wave absorption property. This compound undergoes a charge‐transfer phase transition between FeIII‐CN‐MnII [high temperature (HT) phase] and FeII‐CN‐MnIII [low temperature (LT) phase] near room temperature. The HT phase exhibits a THz wave absorption at 1.06 THz due to the slow vibration of the heavy Rb+ ions encapsulated in the three‐dimensional framework. By contrast, the LT phase displays a higher resonance frequency at 1.13 THz. This shift to a higher frequency is attributed to the framework shrinkage, which decreases the space available for the trapped Rb+ ion. Indeed, the void volume accompanying the phase transition decreases by 16.4 % from 41.4 Å3 (HT phase) to 34.6 Å3 (LT phase). Tuning the THz wave absorption frequency is necessary for THz devices such as absorbers, switches, filters, and modulators, especially for THz technology with THz waves in the sub‐THz region or near 1 THz.
Deionized Water Pretreated Polyimide Nanofiber Membranes as Framework for Polymer Electrolytes Applied to Solid‐State Lithium Metal Batteries
Authors: Liu Yongqi , Chen Zijian , Li Haoyu , Hu Junyu , Xu Zhengbing , Zhu Jinliang 
Published: September 2024

Incorporating a water‐immersed polyimide membrane (WPI) as a framework into a polymer electrolyte PEO/SN/LiTFSI (PSL) can address the challenges faced by solid‐state polymer electrolytes in practical applications for all‐solid‐state lithium‐ion batteries (ASSLIBs) used in flexible and wearable electronics. The inferior mechanical performance and inadequate ionic conductivity of polymer electrolytes have hampered their widespread use. By utilizing a matrix of polyethylene oxide (PEO) along with succinonitrile (SN) and lithium bis(trifluoromethane)sulfonimide (LiTFSI) as additives, the WPI enhances the stability of the PSL electrolyte structure through reinforced internal hydrogen bonds of poly(amic acid), its precursor, post‐thermal imidization in the presence of deionized water. This structural enhancement leads to improved mechanical properties, evidenced by a tensile strength of 12.2 MPa at a high porosity of 85.6%. The WPI‐PSL electrolyte exhibits favorable ionic conductivity, thermal stability, and electrochemical compatibility with lithium metal. As a result, the WPI‐PSL configuration demonstrates exceptional performance in a LiFePO4/Li ASSLIBs system, showcasing outstanding cycling performance at both 30 and 60 °C, with capacity retention ratios reaching 94.6% and 96% after 100 cycles at 0.3 C, respectively. This research significantly advances the development of polymer solid‐state electrolytes, propelling their use in flexible power sources for ASSLIBs.
The impact of digital inequities on salivary gland cancer disparities in the United States
Authors: Bruss David M. , Fei‐Zhang David J. , Kim Helena , Chelius Daniel C. , Sheyn Anthony M. , Maddalozzo John P. , Rastatter Jeffrey C. , D'Souza Jill N. 
Published: September 2024

AbstractIntroductionTechnology and internet access have become increasingly integrated into healthcare as the primary platform for health‐related information and provider‐patient communication. Disparities in access to digital resources exist in the United States and have been shown to impact health outcomes in various head and neck malignancies. Our objective is to evaluate the associations of digital inequity on health outcomes in patients with salivary gland cancer (SGC).MethodsThe Digital Inequity Index (DII) was developed using 17 census‐tract level variables obtained from the American Community Survey and Federal Communications Commission. Variables were categorized as digital infrastructure or sociodemographic (e.g., non‐digital) and scored based on relative rankings across all US counties. Scores were assigned to patients from the Surveillance‐Epidemiology‐End Results (SEER) database diagnosed with SGC between 2013 and 2017 based on county‐of‐residence. Regressions were performed between DII score and outcomes of surveillance time, survival time, tumor stage at time of diagnosis, and treatment modality.ResultsAmong 9306 SGC‐patients, increased digital inequity was associated with advanced‐staging at presentation (OR: 1.04, 95% CI: 1.01–1.07, p = 0.033), increased odds of chemotherapy receipt (OR: 1.05, CI: 1.01–1.10, p = 0.010), and decreased odds of surgical intervention (OR: 0.94, 95% CI: 0.91–0.98, p = 0.003) after accounting for traditional sociodemographic factors. Increased digital inequity was also associated with decreased surveillance time and survival periods.ConclusionsDigital inequity significantly and independently associates with negative health and treatment outcomes in SGC patients, highlighting the importance of directed efforts to address these seldom‐investigated drivers of health disparities.
Head and neck sarcomas: Thirty years of experience in a tertiary referral center in Brazil
Authors: Cattan Marcelo Elias Schempf , Kimura Talita de Carvalho , Lavareze Luccas , Egal Erika Said Abu , Altemani Albina , Mariano Fernanda Viviane 
Published: September 2024

AbstractPurposeThis study analyzed the demographics, clinicopathological, treatment, and survival characteristics of head and neck sarcomas (HNS) diagnosed in a tertiary reference center in Brazil.Materials and methodsHNS cases were retrospectively retrieved from the Department of Pathological Anatomy of the School of Medical Sciences of the State University of Campinas. The medical records were examined to extract demographic, clinicopathological, and follow‐up information. The Pearson chi‐square test, Kaplan–Meier curve, and Cox proportional hazards regression model were employed to identify survival and potential prognostic factors.ResultsA total of 47 patients were included in the study. The majority were men (61.7%) with a mean age of 38.9 years. The nasal cavity (34.0%) was the most common anatomical site. The lesions are usually presented as volume increases (78.7%). The most common histological subtypes were chondrosarcoma, osteosarcoma, and alveolar rhabdomyosarcoma. Surgical excision alone was the most common treatment modality. Local recurrence was observed in 10 cases, and metastases in 3 cases. During a mean follow‐up period of 71.9 months, from diagnosis to the last follow‐up, 31 patients (65.9%) were alive without the disease. A total of 10 patients (21.3%) died of the HNS for a mean follow‐up period of 14.3 months. The time to presentation of more than 6 months (p = 0.0309) and the presence of metastases (p = 0.0315) were identified as prognostic factors for survival, while male sex was found to be an independent prognostic factor for recurrence.ConclusionIn conclusion, the results of this study indicate that the occurrence of a shorter lesion time to presentation and the presence of metastases were associated with a reduction in survival rates in patients with HNS.
Septins: Structural Insights, Functional Dynamics, and Implications in Health and Disease
Authors: Das Aurosikha , Kunwar Ambarish 
Published: September 2024

ABSTRACTSeptins are a class of proteins with diverse and vital roles in cell biology. Structurally, they form hetero‐oligomeric complexes and assemble into filaments, contributing to the organization of cells. These filaments act as scaffolds, aiding in processes like membrane remodeling, cytokinesis, and cell motility. Functionally, septins are essential to cell division, playing essential roles in cytokinetic furrow formation and maintaining the structural integrity of the contractile ring. They also regulate membrane trafficking and help organize intracellular organelles. In terms of physiology, septins facilitate cell migration, phagocytosis, and immune responses by maintaining membrane integrity and influencing cytoskeletal dynamics. Septin dysfunction is associated with pathophysiological conditions. Mutations in septin genes have been linked to neurodegenerative diseases, such as hereditary spastic paraplegias, underscoring their significance in neuronal function. Septins also play a role in cancer and infectious diseases, making them potential targets for therapeutic interventions. Septins serve as pivotal components of intracellular signaling networks, engaging with diverse proteins like kinases and phosphatases. By modulating the activity of these molecules, septins regulate vital cellular pathways. This integral role in signaling makes septins central to orchestrating cellular responses to environmental stimuli. This review mainly focuses on the human septins, their structural composition, regulatory functions, and implication in pathophysiological conditions underscores their importance in fundamental cellular biology. Moreover, their potential as therapeutic targets across various diseases further emphasizes their significance.
Diagnostic Performance of CLEIA Versus FEIA for KL‐6 Peripheral and Alveolar Concentrations in Fibrotic Interstitial Lung Diseases: A Multicentre Study
Authors: d'Alessandro Miriana , Gangi Sara , Paggi Irene , Soccio Piera , Bergantini Laura , Pianigiani Tommaso , Montuori Giusy , Moriondo Giorgia , Natalello Giulio , Marrucci Sofia , Brogna Alfonso , Scioscia Giulia , Lacedonia Donato , Cameli Paolo , Bargagli Elena 
Published: September 2024

ABSTRACTBackgroundInterstitial lung diseases (ILD) is a group of lung disorders characterized by interstitial lung thickening due to inflammatory and fibrotic processes. Krebs von den Lungen‐6 (KL‐6) is a molecule secreted by damaged type II alveolar pneumocytes in the alveolar space. The goal of the present study was to compare two detection methods of KL‐6 in both bronchoalveolar lavage (BAL) and serum from ILD patients at the moment of diagnosis.MethodsPatients with suspicious of ILD and followed at two Italian referral centres for rare lung diseases were included in the study. BAL fluid and serum were collected and analysed by chemiluminescent enzyme immunoassay (CLEIA) and fluorescent enzyme immunoassay (FEIA) methods provided by Tosoh Biosciences.ResultsA total of 158 (mean age ± standard deviation, 61.5 ± 13.7, 65 females) patients were enrolled. A total of, 36 had diagnosis of idiopathic pulmonary fibrosis (IPF), 74 sarcoidosis, 15 connective tissue disease‐ILD (CTD‐ILD) and 33 other ILD. Diagnostic agreement between two methods was demonstrated for both BAL (r = 0.707, p < 0.0001) and serum (r = 0.816, p < 0.0001). BAL KL‐6 values were lower than serum (p < 0.0001). IPF patients had higher serum KL‐6 concentration than other ILDs (p = 0.0294), while BAL KL‐6 values were lower in IPF than in non‐IPF (p = 0.0023).ConclusionThis study explored KL‐6 concentrations through the CLEIA method in serum and BAL of patients with various ILDs, showing significant differences of biomarkers concentrations between IPF and other non‐IPF ILDs. Our findings are promising as they provided further knowledge concerning KL‐6 expression across different ILDs and may suggest its utility in differential diagnosis.
The interactome of cystic fibrosis transmembrane conductance regulator and its role in male fertility: A critical review
Authors: Ribeiro João C. , Rodrigues Bernardo C. , Bernardino Raquel L. , Alves Marco G. , Oliveira Pedro F. 
Published: September 2024

AbstractThe cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic adenosine monophosphate (cAMP)‐regulated chloride and bicarbonate ion channel found in many human cells. Its unique biochemical characteristics and role as a member of the adenosine triphosphate (ATP)‐binding cassette transporters superfamily are pivotal for the transport of several substrates across cellular membranes. CFTR is known to interact, physically and functionally, with several other cellular proteins. Hence, its properties are essential for moving various substances across cell membranes and ensuring correct cell functioning. Genetic mutations or environmental factors may disrupt CFTR's function resulting in different possible phenotypes due to gene variations that affect not only CFTR's function, localization, and processing within cells, but also those of its interactors. This has been reported as an underlying cause of various diseases, including cystic fibrosis. The severe clinical implications of cystic fibrosis have driven intense research into the role of CFTR in lung function but its significance to fertility, particularly in men, has been comparatively understudied. However, ongoing and more recent research into CFTR and its interacting proteins in the testis or specific testicular cells is beginning to shed light on this field. Herein, we provide a comprehensive and up‐to‐date overview of the CFTR, its interactome, and its crucial role in male reproduction, highlighting recent discoveries and advancements in understanding the molecular mechanisms involved. The comprehension of these complex interactions may pave the way for potential therapeutic approaches to improve fertility of men suffering from alterations in the function of CFTR.
Determinants of HBeAg loss during follow‐up of a multiethnic pediatric cohort
Authors: Mutimer David , Atabani Sowsan F. , Brown Maxine , Logan Jacqueline , Kelgeri Chayarani 
Published: September 2024

AbstractHepatitis B e antigen (HBeAg) loss is a key event in the natural history of chronic hepatitis B virus infection. The rate and determinants of HBeAg loss depend upon cohort characteristics at baseline. Few studies have examined the age‐dependent rate, and none have examined the effect of patient sex and ethnicity on the age‐dependant rate. The study of age‐dependent rates requires the identification and long‐term follow‐up of a pediatric cohort. We have studied the age‐dependent rate of HBeAg loss, and the rate of HBeAg loss measured from baseline, in a multi‐ethnic cohort of 454 pediatric patients. During observation, HBeAg loss was observed in 121/303 (39.9%) HBeAg‐positive patients. The rate of HBeAg loss was greater in the second versus the first and third decades of life. The age‐related rate of HBeAg loss was clearly affected by patient sex and ethnicity, with earlier loss observed for males and for White versus both South Asian and Chinese ethnicities. When measured from baseline, Chinese patients had a slower rate of HBeAg loss in comparison with White patients. In multivariate analysis of HBeAg loss during prolonged follow‐up, male sex, older age, and White ethnicity were associated with HBeAg loss, but antiviral treatment was not.
Vertically Integrated Self‐Monitoring AlGaN‐Based Deep Ultraviolet Micro‐LED Array with Photodetector Via a Transparent Sapphire Substrate Toward Stable and Compact Maskless Photolithography Application
Authors: Yu Huabin , Yao Jikai , Memon Muhammad Hunain , Luo Yuanmin , Gao Zhixiang , Luo Dongyang , Wang Rui , Wang Zixun , Chen Wei , Wang Linjun , Li Shuiqing , Zheng Jinjian , Zhang Jiangyong , Liu Sheng , Sun Haiding 
Published: September 2024

AbstractDrawing inspiration from modern integrated circuit systems composed of various electronic components built on a single silicon platform, the emerging integrated photonics can also follow a similar trend in the pursuit of expanded optical functionalities in constructing compact optoelectronic systems. Herein, vertically integrated a micro‐scale light‐emitting diode (micro‐LED) array with a photodetector (PD) side‐by‐side through a transparent sapphire substrate is proposed. The downward emitted photons from the micro‐LEDs can easily transmit through the transparent sapphire and then be captured by the PD fabricated on the backside of the sapphire. Additionally, by integrating a feedback electrical circuit, a self‐stabilized light output power is demonstrated from the micro‐LED array in such vertically integrated LED/PD architecture, which cannot only monitor the fluctuation of light intensity from the micro‐LED array over time but also provide a constant output feedback to ensure a stable light output power. Such a compact and stable DUV light source composed of micro‐LED array is then employed for constructing a DUV maskless photolithography system. To best of our knowledge, this is the first demonstration of maskless photolithography based on DUV micro‐LED active matrix. The proposed vertically‐stacked optical device architecture by leveraging the transparent substrate offers a new path toward the realization of future integrated photonic systems.
Improved expressions of noise parameters for InP HEMTs
Authors: Lyu Yuanting , Li Zhichun , Zhang Ao , Gao Jianjun 
Published: September 2024

AbstractIn this article, we propose a set of new expressions of four noise parameters for InP high electron mobility transistor (HEMT) devices based on PRC noise equivalent circuit model. The effects of gate‐to‐drain capacitance, drain‐to‐source conductance as well as extrinsic resistances have been taken into account. High consistency between measured and modeled noise parameters for 70 nm HEMT with 2 × 30 μm gate width in the frequency range 8–50 GHz are given by the expressions. Great usability is demonstrated for determination of small signal and noise model parameters extraction.