bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic signature of muscle fibre hyperplasia in response to faba bean intake in grass carp
Authors: Yu Er-Meng , Zhang Hao-Fang , Li Zhi-Fei , Wang Guang-Jun , Wu Hong-Kai , Xie Jun , Yu De-Guang , Xia Yun , Zhang Kai , Gong Wang-Bo 
Published: April 2017

AbstractFish muscle growth is important for the rapidly developing global aquaculture industry, particularly with respect to production and quality. Changes in muscle fibre size are accomplished by altering the balance between protein synthesis and proteolysis. However, our understanding regarding the effects of different protein sources on fish muscle proteins is still limited. Here we report on the proteomic profile of muscle fibre hyperplasia in grass carp fed only with whole faba bean. From the results, a total of 99 significantly changed proteins after muscle hyperplasia increase were identified (p < 0.05, ratio <0.5 or >2). Protein–protein interaction analysis demonstrated the presence of a network containing 56 differentially expressed proteins, and muscle fibre hyperplasia was closely related to a protein–protein network of 12 muscle component proteins. Muscle fibre hyperplasia was also accompanied by decreased abundance in the fatty acid degradation and calcium signalling pathways. In addition, metabolism via the pentose phosphate pathway decreased in grass carp after ingestion of faba bean, leading to haemolysis. These findings could provide a reference for the prevention and treatment of human glucose-6-phosphate dehydrogenase deficiency (“favism”).
Environmental dust effects on aluminum surfaces in humid air ambient
Authors: Yilbas Bekir Sami , Hassan Ghassan , Ali Haider , Al-Aqeeli Nasser 
Published: April 2017

AbstractEnvironmental dusts settle on surfaces and influence the performance of concentrated solar energy harvesting devices, such as aluminum troughs. The characteristics of environmental dust and the effects of mud formed from the dust particles as a result of water condensing in humid air conditions on an aluminum wafer surface are examined. The dissolution of alkaline and alkaline earth compounds in water condensate form a chemically active mud liquid with pH 8.2. Due to gravity, the mud liquid settles at the interface of the mud and the aluminum surface while forming locally scattered patches of liquid films. Once the mud liquid dries, adhesion work to remove the dry mud increases significantly. The mud liquid gives rise to the formation of pinholes and local pit sites on the aluminum surface. Morphological changes due to pit sites and residues of the dry mud on the aluminum surface lower the surface reflection after the removal of the dry mud from the surface. The characteristics of the aluminum surface can address the dust/mud-related limitations of reflective surfaces and may have implications for the reductions in the efficiencies of solar concentrated power systems.
Unraveling the role of Calcium ions in the mechanical properties of individual collagen fibrils
Authors: Pang Xiangchao , Lin Lijun , Tang Bin 
Published: April 2017

AbstractCollagen, the dominating material in the extracellular matrix, provides the strength, elasticity and mechanical stability to the organisms. The mechanical property of collagen is mainly dominated by its surrounding environments. However, the variation and origin of the mechanics of collagen fibril under different concentrations of calcium ions (χCa) remains unknown. By using the atomic force microscopy based nanoindentation, the mechanics and structure of individual type II collagen fibril were first investigated under different χCa in this study. The results demonstrate that both of the mechanical and structural properties of the collagen fibril show a prominent dependence on χCa. The mechanism of χCa-dependence of the collagen fibril was attributed to the chelation between collagen molecules and the calcium ions. Given the role of calcium in the pathology of osteoarthritis, the current study may cast new light on the understanding of osteoarthritis and other soft tissue hardening related diseases in the future.
α-MSH and melanocortin receptors at early ontogeny in European sea bass (Dicentrarchus labrax, L.)
Authors: Tsalafouta A. , Gorissen M. , Pelgrim T. N. M. , Papandroulakis N. , Flik G. , Pavlidis M. 
Published: April 2017

AbstractTemporal patterns of whole-body α-MSH concentrations and of transcripts of melanocortin receptors during early development as well as the endocrine response (α-MSH, cortisol, MCR mRNAs) to stress at the end of the larval period were characterized in Dicentrarchus labrax. Immunohistochemistry showed α-MSH positive cells in the pituitary pars intermedia in all stages examined. As development proceeds, α-MSH content gradually increases; mRNA levels of mc2r and mc4r remain low until first feeding where peak values are observed. Mc1r expression was constant during development, pomc mRNA levels remain low until the stage of flexion after which a significant increase is observed. At the stage of the formation of all fins, whole-body cortisol and α-MSH concentrations responded with peak values at 2 h post stress. Additionally, the stress challenge resulted in elevated transcript levels of pomc, mc2r and mc4r but not in mc1r, with a pattern characterized by peak values at 1 h post stress and a strong correlation with whole body α-MSH concentrations was found. Our data provide for the first time a view on the importance of the α-MSH stress response in early development of European sea bass, an additional and relatively poorly understood signal involved in the stress response in teleosts.
Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate
Authors: Morita Masayuki , Takashima Eizo , Ito Daisuke , Miura Kazutoyo , Thongkukiatkul Amporn , Diouf Ababacar , Fairhurst Rick M. , Diakite Mahamadou , Long Carole A. , Torii Motomi , Tsuboi Takafumi 
Published: April 2017

AbstractThe number of malaria vaccine candidates in preclinical and clinical development is limited. To identify novel blood-stage malaria vaccine candidates, we constructed a library of 1,827P. falciparum proteins prepared using the wheat germ cell-free system (WGCFS). Also, a high-throughput AlphaScreen procedure was developed to measure antibody reactivity to the recombinant products. Purified IgGs from residents in malaria endemic areas have shown functional activity against blood-stage parasites as judged by an in vitro parasite Growth Inhibition Assay (GIA). Therefore, we evaluated the GIA activity of 51 plasma samples prepared from Malian adults living in a malaria endemic area against the WGCFS library. Using the AlphaScreen-based immunoreactivity measurements, antibody reactivity against 3 proteins was positively associated with GIA activity. Since anti-LSA3-C responses showed the strongest correlation with GIA activity, this protein was investigated further. Anti-LSA3-C-specific antibody purified from Malian adult plasmas showed GIA activity, and expression of LSA3 in blood-stage parasites was confirmed by western blotting. Taken together, we identified LSA3 as a novel blood-stage vaccine candidate, and we propose that this system will be useful for future vaccine candidate discovery.
MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses
Authors: Shin Chanyoung , Ito Yuma , Ichikawa Shota , Tokunaga Makio , Sakata-Sogawa Kumiko , Tanaka Takashi 
Published: April 2017

AbstractActivation of NF-κB transcription factor is strictly regulated to prevent excessive inflammatory responses leading to immunopathology. However, it still remains unclear how NF-κB activation is negatively controlled. The PDZ-LIM domain-containing protein PDLIM2 is a nuclear ubiquitin E3 ligase targeting the p65 subunit of NF-κB for degradation, thus terminating NF-κB-mediated inflammation. Using yeast two-hybrid screening, we sought to isolate PDLIM2-interacting proteins that are critical for suppressing NF-κB signaling. Here we identified MKRN2, a RING finger domain-containing protein that belongs to the makorin ring finger protein gene family, as a novel p65 ubiquitin E3 ligase. MKRN2 bound to p65 and promoted the polyubiquitination and proteasome-dependent degradation of p65 through the MKRN2 RING finger domain, thereby suppressing p65-mediated NF-κB transactivation. Notably, MKRN2 and PDLIM2 synergistically promote polyubiquitination and degradation of p65. Consistently, MKRN2 knockdown in dendritic cells resulted in larger amounts of nuclear p65 and augmented production of proinflammatory cytokines in responses to innate stimuli. These results delineate a novel role of MKRN2 in negatively regulating NF-κB-mediated inflammatory responses, cooperatively with PDLIM2.
Die Nutzungsbereitschaft von Patienten mit Adipositas gegenüber neuen Medien in der Rehabilitationsnachsorge
Authors: Dorow M. , Löbner M. , Stein J. , Kind P. , Markert J. , Keller J. , Weidauer E. , Riedel-Heller S. 
Published: April 2017

Zusammenfassung
Digitale Medien bieten neue Möglichkeiten für die Rehabilitationsnachsorge. Die Studie untersucht, wie hoch die Bereitschaft von Rehabilitanden ist, neue Medien (SMS, Internet, soziale Netzwerke) im Rahmen einer Nachsorge zu nutzen und welche Faktoren mit der Bereitschaft assoziiert sind.
92 Rehabilitanden (Patienten mit Adipositas) füllten einen Fragebogen zur Nutzungsbereitschaft gegenüber neuen Medien in der Nachsorge aus. Um Einflussfaktoren für die Nutzungsbereitschaft zu identifizieren wurden binär-logistische Regressionsmodelle gerechnet.
Dreiviertel der Rehabilitanden (76,1%) wären bereit, neue Medien in der Nachsorge zu nutzen. Im binär-logistischen Regressionsmodell waren 2 Faktoren signifikant mit der Nutzungsbereitschaft assoziiert: der Besitz eines Smartphones und die Bereitschaft zur telefonischen Beratung.
Die Mehrheit der Rehabilitanden würde neue Medien in der Rehabilitationsnachsorge nutzen. Hinderungsgründe sollten zukünftig genauer erforscht werden.
A Comparison of Age, Size, and Fecundity of Harvested and Reference White Sucker Populations
Authors: Begley Meg , Coghlan Stephen M. , Zydlewski Joseph 
Published: April 2017

Abstract
White Suckers Catostomus commersonii are an important source of fresh bait for the Maine lobster fishery. The Maine Department of Inland Fisheries and Wildlife began issuing commercial harvest permits in 1991, without reporting requirements or limits on the number of permits. There is recent concern that overfishing may be occurring. To infer impact, we investigated demographic differences between White Sucker populations in lakes open to harvest and those in lakes closed to harvest. Each of three harvested lakes was paired to a nearby closed lake as a reference based on general size, morphometry, and information on harvest pressure. In total, 976 spawning White Suckers were collected from the six lakes in 2014 (120–282 individuals/lake). Fish size, estimated age, fecundity, and mortality rates were compared between lakes. We hypothesized that we would find smaller, younger, and less-fecund individuals in harvested lakes compared to reference lakes. Size and age distributions for both sexes differed between nearly all lake pairs (except between males from one pair). White Suckers from reference lakes were larger and older and had greater gonadosomatic indices and fecundity than fish from harvested lakes. Estimated annual mortality rates were at least twofold higher in harvested lakes than in reference lakes. We detected some differences in von Bertalanffy growth parameters between lake pairs, as might occur under selective harvest pressure. The growth coefficient was smaller for reference lakes than for harvested lakes, while asymptotic length was greater for reference lakes than for harvested lakes. The data suggest that current levels of exploitation are resulting in greater age truncation in existing White Sucker populations.
Received September 27, 2016; accepted January 24, 2017 Published online April 4, 2017
MicroRNA signatures associated with thioacetamide-induced liver fibrosis in mice
Authors: Hong Jae-Sang , Lee Do-Hoon , Yook Ye Won , Na Dokyun , Jang Yu Jin , Kim Jong-Hoon , Lee Young Sik 
Published: April 2017

AbstractMultiple etiologies of liver injury are associated with fibrosis in which the key event is the activation of hepatic stellate cells (HSCs). Although microRNAs (miRNAs) are reportedly involved in fibrogenesis, the complete array of miRNA signatures associated with the disease has yet to be elucidated. Here, deep sequencing analysis revealed that compared to controls, 80 miRNAs were upregulated and 21 miRNAs were downregulated significantly in the thioacetamide (TAA)-induced mouse fibrotic liver. Interestingly, 58 of the upregulated miRNAs were localized to an oncogenic miRNA megacluster upregulated in liver cancer. Differential expression of some of the TAA-responsive miRNAs was confirmed, and their human orthologs were similarly deregulated in TGF-β1-activated HSCs. Moreover, a functional analysis of the experimentally validated high-confidence miRNA targets revealed significant enrichment for the GO terms and KEGG pathways involved in HSC activation and liver fibrogenesis. This is the first comprehensive report of miRNAs profiles during TAA-induced mouse liver fibrosis.
Projecting the Water and Electric Consumption of Polytechnic University of the Philippines
Authors: Urrutia Jackie D. , Mercado Joseph , Bautista Lincoln A. , Baccay Edcon B. 
Published: April 2017

Abstract
This study investigates water and electric consumption in Polytechnic University of the Philippines – Sta. Mesa using a time series analysis. The researchers analyzed the water and electric usage separately. Electric consumption was examined in terms of pesos and kilowatt-hour, while water consumption was analyzed in pesos and cubic meter. The data are gathered from the university limited only from January 2009 to July 2015 in a monthly based record. The aim is to forecast the water and electric usage of the university for the years 2016 and 2017. There are two main statistical treatments that the researchers conducted to be able to formulate mathematical models that can estimate the water and electric consumption of the said school. Using Seasonal Autoregressive Integrated Moving Average (SARIMA), electric usage was forecasted in peso and kilowatt-hour, and water usage in peso and cubic meter. Moreover, the predicted values of the consumptions are compared to the actual values using Paired T-test to examine whether there is a significant difference. Forecasting accurately the water and electric consumption would be helpful to manage the budget allotted for the water and electric consumption of PUP – Sta. Mesa for the next two years.
An Analysis on the Unemployment Rate in the Philippines: A Time Series Data Approach
Authors: Urrutia J D , Tampis R L , Atienza JB E 
Published: April 2017

Abstract
This study aims to formulate a mathematical model for forecasting and estimating unemployment rate in the Philippines. Also, factors which can predict the unemployment is to be determined among the considered variables namely Labor Force Rate, Population, Inflation Rate, Gross Domestic Product, and Gross National Income. Granger-causal relationship and integration among the dependent and independent variables are also examined using Pairwise Granger-causality test and Johansen Cointegration Test. The data used were acquired from the Philippine Statistics Authority, National Statistics Office, and Bangko Sentral ng Pilipinas. Following the Box-Jenkins method, the formulated model for forecasting the unemployment rate is SARIMA (6, 1, 5) × (0, 1, 1)4 with a coefficient of determination of 0.79. The actual values are 99 percent identical to the predicted values obtained through the model, and are 72 percent closely relative to the forecasted ones. According to the results of the regression analysis, Labor Force Rate and Population are the significant factors of unemployment rate. Among the independent variables, Population, GDP, and GNI showed to have a granger-causal relationship with unemployment. It is also found that there are at least four cointegrating relations between the dependent and independent variables.
Using Ag/Ag2O/SnO2 Nanocomposites to Remove Malachite Green by a Photocatalytic Process
Authors: Taufik A. , Paramarta V. , Prakoso S.P. , Saleh R. 
Published: April 2017

Abstract
Silver/silver oxide/tin oxide nanocomposites of various weight ratios were synthesized using a microwave-assisted method. The Ag/Ag2O:SnO2 nanoparticle weight ratios used were 25:75, 50:50, and 75:25. All samples were characterized using X-ray diffraction, UV-Vis spectroscopy, Differential Scanning Calorimetry and Thermogravimetric Analysis (TGA). The Ag/Ag2O/SnO2 nanocomposites contained cubic structures provided by the Ag and Ag2O and tetragonal structures provided by the SnO2. The silver resulted in surface plasmon resonance (SPR) at a wavelength of about 435 nm. The silver oxide material was transformed into pure Ag at a temperature of about 370 °C The photocatalytic activity was tested on the degradation of malachite green (MG) from an aqueous solution. The results showed that Ag/Ag2O/SnO2 at a ratio of 50:50 exhibited the best photocatalytic performance for degrading MG under visible-light irradiation. The degradation of MG using Ag/Ag2O/SnO2 nanocomposites followed pseudo first-order kinetic reactions, and electron holes were found to be the main species acting on the degradation process.
Photodegradation of Methylene Blue by LaFeO3/ZnO Nanocomposites under Visible and UV Light Irradiation
Authors: Afifah Nur , Saleh Rosari 
Published: April 2017

Abstract
In this study, LaFeO3/ZnO nanocomposites with different molar ratios of ZnO (0.1, 0.3, 0.5, and 1) have been prepared using the sol-gel method. The structural and elemental analyses of the samples were measured by X-ray diffraction (XRD) and X-ray fluorescence (XRF) spectroscopy. XRD results showed that the synthesized samples exhibited an orthorhombic structure of LaFeO3 and a hexagonal wurtzite structure of ZnO. However, the ZnO hexagonal wurtzite structure could not be detected below the ZnO molar ratio of 0.5. The presence of ZnO on the nanocomposites was confirmed using XRF measurements. The results revealed that the XRF spectra of the samples consisted of La, Fe, and Zn atoms. The photodegradation of methylene blue (MB) using LaFeO3/ZnO nanocomposites was then monitored under visible and UV light irradiation. The photodegradation results showed that the presence of LaFeO3/ZnO nanocomposites could degrade the MB solution. The highest MB degradation was found in the presence of ZnO at a molar ratio of 0.5. All of the LaFeO3/ZnO nanocomposites showed better photocatalytic performance than did LaFeO3 nanoparticles alone. Furthermore, several parameters, such as the effect of catalyst dose, initial concentration of MB, and various radical scavengers, were also measured to obtain the maximum condition and the main active species involved in the photocatalytic process.
Development of Fe3O4/ZrO2 Composite Powered by Nanographene Platelets (NGP) for Degradation of Water Pollutants via Photo- and Sonocatalysis
Authors: Kristianto Yogi , Taufik Ardiansyah , Saleh Rosari 
Published: April 2017

Abstract
In this study, a series of Fe3O4/ZrO2/nanographene platelets (NGP) composite, with various weight percent (wt%) of NGP (5%, 10% and 15%), were prepared successfully using ultrasonic-assisted followed by simple hydrothermal method. Their physicochemical properties were fairly characterized by X-ray diffraction, fourier transform infrared and thermal gravimetric analysis. Furthermore, their catalytic activities were investigated toward anionic congo red (CR) and cationic methylene blue (MB) as models of organic pollutant under ultraviolet (UV) and ultrasonic (US) irradiation, respectively. The experimental results showed that the incorporation of NGP in Fe3O4/ZrO2 composite improved its efficiency in degrading CR and MB and became maximum at 10wt% of NGP. In addition, the role of active radicals involved in catalytic activities were discussed.
RTK: efficient rarefaction analysis of large datasets
Authors: Saary Paul , Forslund Kristoffer , Bork Peer , Hildebrand Falk 
Published: April 2017

Abstract

Motivation
The rapidly expanding microbiomics field is generating increasingly larger datasets, characterizing the microbiota in diverse environments. Although classical numerical ecology methods provide a robust statistical framework for their analysis, software currently available is inadequate for large datasets and some computationally intensive tasks, like rarefaction and associated analysis.

Results
Here we present a software package for rarefaction analysis of large count matrices, as well as estimation and visualization of diversity, richness and evenness. Our software is designed for ease of use, operating at least 7x faster than existing solutions, despite requiring 10x less memory.

Availability and Implementation
C ++ and R source code (GPL v.2) as well as binaries are available from https://github.com/hildebra/Rarefaction and from CRAN (https://cran.r-project.org/).

Supplementary information
Supplementary data are available at Bioinformatics online.
Ipsilateral Pupillary Dilation Following Carotid Endarterectomy: A Temporary and Benign Phenomenon
Authors: Steinberg Jeffrey A. , Carter Bob S. , Lee Marco B. , Steinberg Gary K. 
Published: April 2017

Abstract

BACKGROUND AND IMPORTANCE: Cases of post carotid endarterectomy (CEA) Horner's syndrome have been reported, with symptoms attributed to manipulation of the sympathetic plexus situated along the carotid artery; however, these patients presented with the typical constricted pupil. We report the first 3 cases to our knowledge of mydriasis following CEA.

CLINICAL PRESENTATION: We present 3 cases of CEA followed by immediate postoperative development of ipsilateral mydriasis. The patients were otherwise at their neurologic baseline and the mydriasis resolved over the ensuing few days.

CONCLUSION: We suggest that these cases are secondary to an ischemic phenomenon, specifically to parasympathetic structures such as the ciliary ganglion and/or oculomotor nerve, resulting in autonomic dysfunction manifested by pupillary dilation. A similar finding of mydriasis occurring subsequent to other carotid pathology has been reported, with ischemia to parasympathetic structures also proposed as the underlying etiology. Although pupillary dilation often represents a worrisome neurosurgical sign indicating herniation, it should be recognized that after CEA this finding may be a transient, benign occurrence.