bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy metabolic pathways control the fate and function of myeloid immune cells
Authors: Al-Khami Amir A , Rodriguez Paulo C , Ochoa Augusto C 
Published: May 2017

Abstract
The past decade has seen a significant interest in investigating the intracellular metabolism of cells of the immune system. This has increased the realization that immune cells endure metabolic reprogramming upon responding to pathogen-derived or inflammatory signals. More importantly, not only does this metabolic switch provide for the bioenergetic and biosynthetic demands but also it, in a highly specific manner, determines the cellular fate and function. In this review, we discuss the metabolic aspects that regulate the differentiation and function of myeloid cells, pivotal for both innate and adaptive immunity. The manipulation of these pathways can alter the function of these cells and therefore, could provide novel therapeutic approaches in cancer and other chronic inflammatory conditions.
Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airway dysfunction with type 2 inflammation
Authors: Ochkur Sergei I , Doyle Alfred D , Jacobsen Elizabeth A , LeSuer William E , Li Wen , Protheroe Cheryl A , Zellner Katie R , Colbert Dana , Shen HuaHao H , Irvin Charlie G , Lee James J , Lee Nancy A 
Published: May 2017

Abstract
Eosinophils and the release of cationic granule proteins have long been implicated in the development of the type 2–induced pathologies linked with respiratory inflammation. Paradoxically, the ablation of the two genes encoding the most abundant of these granule proteins, major basic protein-1 (MBP-1) and eosinophil peroxidase (EPX), results in a near collapse of eosinophilopoiesis. The specificity of this lineage ablation and the magnitude of the induced eosinopenia provide a unique opportunity to clarify the importance of eosinophils in acute and chronic inflammatory settings, as well as to identify potential mechanism(s) of action linked with pulmonary eosinophils in those settings. Specifically, we examined these issues by assessing the induced immune responses and pathologies occurring in MBP-1−/−/EPX−/− mice after 1) ovalbumin sensitization/provocation in an acute allergen-challenge protocol, and 2) crossing MBP-1−/−/EPX−/− mice with a double-transgenic model of chronic type 2 inflammation (i.e., I5/hE2). Acute allergen challenge and constitutive cytokine/chemokine expression each induced the accumulation of pulmonary eosinophils in wild-type controls that was abolished in the absence of MBP-1 and EPX (i.e., MBP-1−/−/EPX−/− mice). The expression of MBP-1 and EPX was also required for induced lung expression of IL-4/IL-13 in each setting and, in turn, the induced pulmonary remodeling events and lung dysfunction. In summary, MBP-1−/−/EPX−/− mice provide yet another definitive example of the immunoregulatory role of pulmonary eosinophils. These results highlight the utility of this unique strain of eosinophil-deficient mice as part of in vivo model studies investigating the roles of eosinophils in health and disease settings.
Crosstalk from cAMP to ERK1/2 emerges during postnatal maturation of nociceptive neurons and is maintained during aging
Authors: Isensee Joerg , Schild Cosimo , Schwede Frank , Hucho Tim 
Published: May 2017

ABSTRACT
Maturation of nociceptive neurons depends on changes in transcription factors, ion channels and neuropeptides. Mature nociceptors initiate pain in part by drastically reducing the activation threshold via intracellular sensitization signaling. Whether sensitization signaling also changes during development and aging remains so far unknown. Using a novel automated microscopy approach, we quantified changes in intracellular signaling protein expression and in their signaling dynamics, as well as changes in intracellular signaling cascade wiring, in sensory neurons from newborn to senescent (24 months of age) rats. We found that nociceptive subgroups defined by the signaling components protein kinase A (PKA)-RIIβ (also known as PRKAR2B) and CaMKIIα (also known as CAMK2A) developed at around postnatal day 10, the time of nociceptor maturation. The integrative nociceptor marker, PKA-RIIβ, allowed subgroup segregation earlier than could be achieved by assessing the classical markers TRPV1 and Nav1.8 (also known as SCN10A). Signaling kinetics remained constant over lifetime despite in part strong changes in the expression levels. Strikingly, we found a mechanism important for neuronal memory – i.e. the crosstalk from cAMP and PKA to ERK1 and ERK2 (ERK1/2, also known as MAPK3 and MAPK1, respectively) – to emerge postnatally. Thus, maturation of nociceptors is closely accompanied by altered expression, activation and connectivity of signaling pathways known to be central for pain sensitization and neuronal memory formation.
A dual phenotype of MDA-MB-468 cancer cells reveals mutual regulation of tensin3 and adhesion plasticity
Authors: Veß Astrid , Blache Ulrich , Leitner Laura , Kurz Angela R. M. , Ehrenpfordt Anja , Sixt Michael , Posern Guido 
Published: May 2017

ABSTRACT
A change regarding the extent of adhesion − hereafter referred to as adhesion plasticity − between adhesive and less-adhesive states of mammalian cells is important for their behavior. To investigate adhesion plasticity, we have selected a stable isogenic subpopulation of human MDA-MB-468 breast carcinoma cells growing in suspension. These suspension cells are unable to re-adhere to various matrices or to contract three-dimensional collagen lattices. By using transcriptome analysis, we identified the focal adhesion protein tensin3 (Tns3) as a determinant of adhesion plasticity. Tns3 is strongly reduced at mRNA and protein levels in suspension cells. Furthermore, by transiently challenging breast cancer cells to grow under non-adherent conditions markedly reduces Tns3 protein expression, which is regained upon re-adhesion. Stable knockdown of Tns3 in parental MDA-MB-468 cells results in defective adhesion, spreading and migration. Tns3-knockdown cells display impaired structure and dynamics of focal adhesion complexes as determined by immunostaining. Restoration of Tns3 protein expression in suspension cells partially rescues adhesion and focal contact composition. Our work identifies Tns3 as a crucial focal adhesion component regulated by, and functionally contributing to, the switch between adhesive and non-adhesive states in MDA-MB-468 cancer cells.
The nuclear export factor CRM1 controls juxta-nuclear microtubule-dependent virus transport
Authors: Wang I-Hsuan , Burckhardt Christoph J. , Yakimovich Artur , Morf Matthias K. , Greber Urs F. 
Published: May 2017

ABSTRACT
Transport of large cargo through the cytoplasm requires motor proteins and polarized filaments. Viruses that replicate in the nucleus of post-mitotic cells use microtubules and the dynein–dynactin motor to traffic to the nuclear membrane and deliver their genome through nuclear pore complexes (NPCs) into the nucleus. How virus particles (virions) or cellular cargo are transferred from microtubules to the NPC is unknown. Here, we analyzed trafficking of incoming cytoplasmic adenoviruses by single-particle tracking and super-resolution microscopy. We provide evidence for a regulatory role of CRM1 (chromosome-region-maintenance-1; also known as XPO1, exportin-1) in juxta-nuclear microtubule-dependent adenovirus transport. Leptomycin B (LMB) abolishes nuclear targeting of adenovirus. It binds to CRM1, precludes CRM1–cargo binding and blocks signal-dependent nuclear export. LMB-inhibited CRM1 did not compete with adenovirus for binding to the nucleoporin Nup214 at the NPC. Instead, CRM1 inhibition selectively enhanced virion association with microtubules, and boosted virion motions on microtubules less than ∼2 µm from the nuclear membrane. The data show that the nucleus provides positional information for incoming virions to detach from microtubules, engage a slower microtubule-independent motility to the NPC and enhance infection.
Polystyrene-Cross-Linking Ortho-Substituted Triphenylphosphines: Synthesis, Coordination Properties, and Application to Pd-Catalyzed Cross-Coupling of Aryl Chlorides
Authors: Iwai Tomohiro , Asano Kiichi , Harada Tomoya , Sawamura Masaya 
Published: May 2017

Abstract
Polystyrene-cross-linking triphenylphosphines having methyl groups as ortho substituents were synthesized. Coordination of the polymer-bound tri(o-tolyl)phosphine-type ligand toward a Pd(II) complex was investigated by 31P CP/MAS NMR spectroscopies. Effects of the ortho-methyl-substituent were evaluated in the Pd-catalyzed cross-coupling of aryl chlorides.
A Debtor’s Petition, 1676
Authors: Thrush Coll 
Published: May 2017

Come listen to me and I will declare
a story as true as you ever did hear
I merry will be while my money doth last
and only will shew that the worst is past.

King’s Bench. Queen’s Bench. Wood Street. Poultry. Fleet. Ludgate. Tothill Fields. Coldbath Fields....
A Lost Museum, 1793
Authors: Thrush Coll 
Published: May 2017

Sparry bodies, eagle stones, Muscovy mica, ponderous earths; garnets of twenty-six sides, violet fluors, Brazil rubies, Peru emeralds.

Eora people are singing, Eora people are dreaming, Eora people are stick-firing the land, Eora people are seeing the ships come in. Eora people are greeting the newcomers, Eora people...
Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs
Authors: Lin H. , Zhou J. , Cao L. , Wang H.R. , Dong J. , Chen Z.R. 
Published: May 2017

Objectives The lack of effective treatment for cartilage defects has prompted investigations using tissue engineering techniques for their regeneration and repair. The success of tissue-engineered repair of cartilage may depend on the rapid and efficient adhesion of transplanted cells to a scaffold. Our aim in this study was to repair full-thickness defects in articular cartilage in the weight-bearing area of a porcine model, and to investigate whether the CD44 monoclonal antibody biotin-avidin (CBA) binding technique could provide satisfactory tissue-engineered cartilage. Methods Cartilage defects were created in the load-bearing region of the lateral femoral condyle of mini-type pigs. The defects were repaired with traditional tissue-engineered cartilage, tissue-engineered cartilage constructed with the biotin-avidin (BA) technique, tissue-engineered cartilage constructed with the CBA technique and with autologous cartilage. The biomechanical properties, Western blot assay, histological findings and immunohistochemical staining were explored. Results The CBA group showed similar results to the autologous group in biomechanical properties, Moran’s criteria, histological tests and Wakitani histological scoring. Conclusions These results suggest that tissue-engineered cartilage constructed using the CBA technique could be used effectively to repair cartilage defects in the weight-bearing area of joints. Cite this article: H. Lin, J. Zhou, L. Cao, H. R. Wang, J. Dong, Z. R. Chen. Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs. Bone Joint Res 2017;6:–295. DOI: 10.1302/2046-3758.65.BJR-2016-0277.
Free-Circulating Methylated DNA in Blood for Diagnosis, Staging, Prognosis, and Monitoring of Head and Neck Squamous Cell Carcinoma Patients: An Observational Prospective Cohort Study
Authors: Schröck Andreas , Leisse Annette , de Vos Luka , Gevensleben Heidrun , Dröge Freya , Franzen Alina , Wachendörfer Malin , Schröck Friederike , Ellinger Joerg , Teschke Marcus , Wilhelm-Buchstab Timo , Landsberg Jennifer , Holdenrieder Stefan , Hartmann Gunther , Field John K , Bootz Friedrich , Kristiansen Glen , Dietrich Dimo 
Published: May 2017

Abstract

BACKGROUND
Circulating cell-free DNA methylation testing in blood has recently received regulatory approval for screening of colorectal cancer. Its application in other clinical settings, including staging, prognosis, prediction, and recurrence monitoring is highly promising, and of particular interest in head and neck squamous cell carcinomas (HNSCCs) that represent a heterogeneous group of cancers with unsatisfactory treatment guidelines.

METHODS
Short stature homeobox 2 (SHOX2) and septin 9 (SEPT9) DNA methylation in plasma from 649 prospectively enrolled patients (training study: 284 HNSCC/122 control patients; testing study: 141 HNSCC/102 control patients) was quantified before treatment and longitudinally during surveillance.

RESULTS
In the training study, 59% of HNSCC patients were methylation-positive at 96% specificity. Methylation levels correlated with tumor and nodal category (P &lt; 0.001). Initially increased methylation levels were associated with a higher risk of death [SEPT9: hazard ratio (HR) = 5.27, P = 0.001; SHOX2: HR = 2.32, P = 0.024]. Disease recurrence/metastases were detected in 47% of patients up to 377 days earlier compared to current clinical practice. The onset of second cancers was detected up to 343 days earlier. In the testing study, sensitivity (52%), specificity (95%), prediction of overall survival (SEPT9: HR = 2.78, P = 0.022; SHOX2: HR = 2.50, P = 0.026), and correlation with tumor and nodal category (P &lt;0.001) were successfully validated.

CONCLUSIONS
Methylation testing in plasma is a powerful diagnostic tool for molecular disease staging, risk stratification, and disease monitoring. Patients with initially high biomarker levels might benefit from intensified treatment and posttherapeutic surveillance. The early detection of a recurrent/metastatic disease or a second malignancy could lead to an earlier consecutive treatment, thereby improving patients' outcomes.
Kinesiologically examination of the activation rates of some muscles used in Zeybek folk dance performances<p>Zeybek halk dansları icrasında kullanılan bazı kasların aktivasyon oranlarının kinesiyolojik açıdan incelenmesi
Authors: Gülbeyaz Kürşad 
Published: May 2017

Many agonist, antagonist and synergist lower and upper extremity muscles work in coordination performing the Turkish folk dances as a bodily activity. It is important to find out which muscles are active and how do they work in performing Zeybek as type of Turkish folk dance that is important for the movement analysis of the style. The literature findings shown that the researches have been done in this field very limited; thus, we believe this study will contribute to fulfill the need.Turkish folk dances have a broad variety of style, movement and motives, there for, the study is limited to only Aegean region folk dance of zeybek style and certain dances, deliberately, have been chosen for the study and the activation rate of muscles used in the chosen “zeybek” dances have been examined with EMG.Extended English abstract is in the end of PDF (TURKISH) file.ÖzetBedensel bir aktivite olması nedeniyle, Türk halk danslarının icrasında birçok agonist, antogonist ve sinirgest alt ve üst ekstremite kas grubunun aynı anda koordineli bir şekilde çalışması söz konusudur. Bu kasların hangileri olduğu ve ne oranda aktif olduklarının tespit edilmesi, Türk halk danslarının bedensel bir çalışma olarak, kas gelişimine olan etkisi açısından önemli bir bilgidir. Türkiye’deki literatür incelendiğinde bu konu ile ilgi araştırmaların kısıtlı olduğu görülmektedir. Bu özgün çalışma görülen bu kısıtlılığı gidermeye katkı amacını taşımaktadır.Çalışma Türk halk danslarının çok geniş bir çeşitliliğe sahip olması nedeniyle ve bedensel hareket kapsamının çok geniş olması bakımından, özellikle zeybek danslarına özgü kas gruplarının aktivasyon oranlarını incelemektedir. Bu bağlamda, çalışma özellikle seçilen dansların hareketlerini gerçekleştiren kaslara, EMG sistemi uygulanarak, aktivasyon oranlarına ulaşılmıştır.
Namesake and Inheritance
Authors: Marquis Christine Luckritz 
Published: May 2017

This chapter investigates how the intersection of names(akes), legacies, and social locations associated with the monastic tradition were negotiated in light of the first Origenist controversy. It takes as its starting point the simultaneously rather common (from a Roman senatorial family) and rather unusual (within the monastic milieu) situation in which the legacies of Melania the Elder and her namesake, Melania the Younger, are found. While Melania the Younger did follow in her grandmother's footsteps, fleeing Rome (though under decidedly different circumstances) and taking up the monastic life, the value of her familial position was much more mixed in light of her grandmother's decided Origenism. Whereas the monastic tradition would sometimes blur the lives and sayings of Macarii, Isaiahs, Apollos, and the like, both the Lausiac History and the Life of Melania the Younger are careful to distinguish between the Melanias, due to the grandmother's Origenist legacy.
Semiretículos y objetos k-finitos
Authors: Acuña-Ortega Osvaldo 
Published: May 2017

Se prueba que si X es un objeto de un topos elemental entonces las propiedades siguientes son equivalentes: (a) X es K-finito, (b) para todo semiretículo con uniones binarias B, la diagonal B→ BX tiene un adjuntoizquierdo.
Interés por el gobierno y gobierno a través del interés: constitución de la naturaleza infantil
Authors: Marín-Díaz Dora Lilia 
Published: May 2017

El artículo propone una lectura histórica de la forma como fue creada discursivamente la naturaleza infan-til. Se señala que fue a través de la invención y uso de nociones como desarrollo, interés, libertad, entre otras, que esa naturaleza infantil emergió y se plantea que tal intervención expresa la articulación de tres formas de practicar y pensar la vida en las sociedades occidentales –discursos liberales, discursos naturalistas y prácticas disciplinarias. La articulación de esos tres elementos aconteció durante lo que se conoce como Modernidad, momento en el que se organizaron formas de gobierno vinculadas a la idea de libertad. Palabras claveAprendizaje, naturaleza infantil, gobierno.
Os Pintores de W. G. Sebald: a função das belas artes na sua obra em prosa
Authors: Fuchs Anne 
Published: May 2017

Há uma literatura crescente acerca do uso da fotografia por Sebald, que aborda uma certa ambiguidade ontológica que abrange a maior parte das fotos contidas em sua obra. Em Sebald, fotografias tendem a exceder o código simbólico das narrativas; elas frequentemente desestabilizam as relações que se estabelecem entre texto e imagem, vida e morte, trauma e história, lembrança e esquecimento. Nesse contexto, o presente artigo enfoca o tratamento que Sebald deu às Belas Artes em seus escritos, argumentando que na prosa sebaldiana a distinção entre arte e fotografia é significativa tanto em termos estéticos como epistemológicos, e deve, consequentemente, receber uma análise mais detalhada. Enquanto a fotografia tende a explorar as relações entre história e trauma por meio do convite à averiguação do seu próprio estatuto representacional, as obras de arte erudita que figuram na prosa de Sebald se oferecem como um porto seguro para a contemplação terapêutica, um contraponto que manifesta momentos de transcendência. O argumento se desenvolve fazendo referência ao importante ensaio de Sebald sobre o pintor Jan Peter Tripp, e as releituras das obras primas que figuram em Schwindel.Gefühle., Die Ausgewanderten, Die Ringe des Saturn e Austerlitz.
Localización de una plataforma de cross docking en el contexto de logística focalizada utilizando distancias euclidianas
Authors: Kalenatic Dusko , López Bello Cesar Amilcar , González Rodríguez Leonardo José , Rueda Velasco Feizar Javier 
Published: May 2017

El actual contexto de los escenarios globales de defensa ha impulsado el desarrollo de conceptos cómo el de logística focalizada, creado para brindar soporte logístico en ambientes de alta volatilidad e incertidumbre. Dentro de ese marco la logística focalizada requiere de herramientas que permitan agilizar los procesos de toma de decisiones en donde la modelación matemática es útil como herramienta de soporte. En orden a lo anterior, se presenta un modelo que pretende soportar la decisión de localización de una plataforma de cross-docking y asignación de medios de transporte utilizando distancias euclidianas de tal manera que se permita garantizar una distribución ágil desde orígenes de suministro hasta los orígenes de demanda siendo especialmente novedosa la estratégica de solución del mismo.
SITE SUITABILITY ANALYSIS FOR SOLID WASTE LANDFILL SITE LOCATION USING GEOGRAPHIC INFORMATION SYSTEMS AND REMOTE SENSING: A CASE STUDY OF BANKET TOWN BOARD, ZIMBABWE
Authors: Jerie Steven 
Published: May 2017

<p>The aim of this study was to map the optimal sites that were environmentally suitable for locating a dumpsite in the Zimbabwean town of Banket as well as investigating the suitability of the dumpsite being currently used by the Banket Town Board. Geographic Information Systems and remote sensing constituted the main quantitative techniques used in data collection. Digitizing, buffering and overlaying were used to map suitable sites for locating a dumpsite in Banket. Environmental parameters were extracted from Google Earth by way of digitizing, buffered using Quantum GIS 2.8 and then overlaid using Quantum GIS 2.8 to produce the suitability map. In support of the quantitative techniques, qualitative techniques that included interviews, questionnaires, observations and literature were used to compliment the quantitative methods. Qualitative techniques comprised of knowledge accessed through interviews from the Banket Town Board, Environmental Management Agency and literature from which significant environmental parameters were drawn and their buffer distances as well as environmental effects of the current dumpsite in Banket. Results of the research presented a suitability map which showed the possible suitable sites for establishing a dumpsite in Banket as well as the most considered suitable site in the eastern part of the town. In mapping the suitable sites according to a number of buffered parameters (Central Business District, Railway line, Road, and Rivers) the current dumpsite was found to lie within the parameters designated as unsuitable. It was recommended that Banket should consider closing its current dumpsite for a better location from the possible suitable sites established by the study especially the site on the eastern part of the town which is suitable in terms of all buffered parameters as well as other confounding variables that include economy, accessibility and elevation as well as slope. </p>
Reducing Cosmopolites sordidus populations and damage using traps baited with pheromone and plantain corm
Authors: Osorio-Osorio Rodolfo , López-Naranjo José Isabel , Salinas-Hernández Rosa Ma. , De la Cruz-Lázaro Efraín , Márquez-Quiroz Cesar , Cibrián-Tovar Juan 
Published: May 2017

The banana weevil Cosmopolites sordidus is an important pest in plantain and banana crops throughout the world. The objective was to evaluate a mass trapping system based on traps baited with synthetic pheromone (sordidin) + plantain corm tissue to reduce C. sordidus populations and damage in the Centro-Chontalpa region of Tabasco state, Mexico. The experiment was carried out on plots of about two to three hectares of Horn plantain Musa AAB infested by C. sordidus. Treatments were four traps ha-1, eight traps ha-1 and a control (without traps). Population density and damage in corms of small plants (suckers) were reduced after six months of mass trapping, while damage in corms of mature plants (freshly harvested) only decreased signi cantly after 13 months of trapping. By doubling the number of traps, the reduction in the C. sordidus population and damage was not signi cant. The use of four traps ha-1 baited with synthetic pheromone + plantain corm is a technically feasible strategy that provides at least partial control of this pest under local Horn plantain production conditions.