bluuebunny/crossref_metadata_2025_split huggingface dataset:
FUNDUS AUTOFLUORESCENCE FINDINGS IN EARLY CHLOROQUINE MACULOPATHY
Authors: Bergholz Richard , Rossel Mirjam , Schroeter Jan , Dutescu Ralf M. , Salchow Daniel J. 
Published: October 2016

Background/Purpose:
Toxic maculopathy is a rare but severe complication of chloroquine intake. Although the phenotype of established maculopathy was analyzed in detail, few data exist on the ophthalmologic findings in the early stage.

Methods:
The authors present 4 female patients with unequivocal signs of chloroquine maculopathy (bilateral [peri-]central scotoma in the visual fields, structural alterations of the macula in the optical coherence tomography) but normal or atypical findings as to fundus autofluorescence imaging.

Results:
In all patients, optical coherence tomography showed perifoveal thinning and subtle alterations of the outer retinal layers. In one patient, the latter was limited to the pericentral region, whereas it showed a more diffuse distribution in two other patients. One patient showed a combination of pericentral and diffuse damage. Multifocal electroretinography was recorded in three patients and revealed the typical (peri-)central amplitude reductions.

Conclusion:
A normal fundus autofluorescence does not rule out toxic maculopathy. Optical coherence tomography and multifocal electroretinography seem to be more sensitive in the early stage of the disease. In case of typical complaints and visual fields, the optical coherence tomography has to be evaluated for subtle alterations of the outer retina. Their presence justifies the cessation of the drug. Multifocal electroretinography may be of special value in these problematic cases.
BILATERAL CHOROIDAL NEOVASCULARIZATION IN A PATIENT WITH BETA-THALASSEMIA MAJOR AND ITS MANAGEMENT
Authors: Kiarudi Mohammad Yaser , Fadakar Kaveh , Mousavi Ebrahim 
Published: October 2016

Purpose:
To describe a case of bilateral choroidal neovascularization in a patient with beta-thalassemia major and an interventional therapeutic attempt.

Method:
Retrospective case report.

Result:
A known case of beta-thalassemia major presented with gradual vision loss. Fundoscopy revealed bilateral macular edema accompanied by intraretinal hemorrhages. Optical coherence tomography and fluorescein angiography were compatible with the diagnosis of choroidal neovascularization. No signs of angioid streaks or other related abnormalities were noticed in examination and imaging. The patient was treated with intravitreal bevacizumab, and visual acuity improved within 6 months of follow-up.

Conclusion:
Bilateral choroidal neovascularization can be a presenting pathology in patients with beta-thalassemia major. Anti–vascular endothelial growth factor is a promising treatment option.
Relationship of Psychosocial Resources With Allostatic Load: A Systematic Review
Authors: Wiley Joshua F. , Bei Bei , Bower Julienne E. , Stanton Annette L. 
Published: October 2016

ABSTRACT

Objective
Allostatic load (AL) represents cumulative wear-and-tear on the body and is operationalized as a multisystem index of biomarkers. Allostatic load is associated with morbidities and mortality, leading to a growing body of literature that uses AL as an outcome in its own right. Psychosocial resources (PSRs), such as mastery and social support, may influence health outcomes in part via AL, and the current review seeks to characterize the relations between PSRs and AL.

Methods
A systematic review was conducted by searching PubMed, CINAHL Plus, PsycINFO, Scopus, and Embase for studies examining the relation between PSR(s) and AL in humans. From 1,417 abstracts screened, 60 full-text articles were reviewed, and 24 studies met inclusion criteria.

Results
Mixed evidence exists for a relationship between PSRs and AL. Most (14/24) studies used a cross-sectional design, and only one study investigated whether a PSR predicted change in AL. Compared to cross-sectional studies, longitudinal studies were more likely to report a significant relationship (8/14 versus 8/10, respectively). Studies with statistically significant main or moderated effects had larger sample sizes than those reporting null effects. Whether a study reported a significant main or moderated relationship did not differ by whether psychological (8/11) or social (10/16) resources were assessed.

Conclusions
Evidence for a relationship between PSRs and AL is equivocal, and obtained significant relationships are generally small in magnitude. Gaps in the current literature and directions for future research are discussed. Longitudinal studies are needed that repeatedly assess PSRs and AL.
Physicians and Students Take to the Streets to Ask: What Do People Want From Their Health Care?
Authors: DiGioia Kimberly , Nair Mohit , Shields Morgan , Saini Vikas 
Published: October 2016

Abstract
With the aim of better understanding what the public (as opposed to “patients”) wants from health care, this study asked people on the street, “What does the right health care mean to you?” Responses ranged from “Caring about me more than just in the appointment” to “That everyone should see exactly what medical treatment costs.” A qualitative analysis revealed that all responses fell into 2 overarching categories: health care at the interpersonal level and health care at the system level. Approximately 66.7% of responses included system-level factors, whereas 59% of responses included interpersonal-level factors. We conclude that the public is cognizant of and concerned about issues that also concern patients and others working to improve health care and, thus, should be engaged in the process to design care in a way that meets their needs and preferences before they become ill or interact with the delivery system.
Computed Tomographic Appearance of Organizing Pneumonia in an Oncologic Patient Population
Authors: Long Niamh M. , Plodkowski Andrew J. , Schor-Bardach Rachel , Geyer Alexander I. , Zheng Junting , Moskowitz Chaya S. , Ginsberg Michelle S. 
Published: October 2016

Objective
The aims of this study were to describe the computed tomographic features of organizing pneumonia (OP) in an oncologic patient population and to also identify features associated with lung cancer and patients undergoing hematopoietic stem cell transplant (HSCT).

Methods
In retrospective computed tomographies from 151 patients with pathologically confirmed OP between January 2009 and September 2014, number of lesions, location, size, margin type, and consistency, as well as volume of lymphadenopathy and the presence and size of pleural effusions, were recorded. Associated malignancy was noted.

Results
Organizing pneumonia most commonly presented as a diffuse process (n = 62, 41%), frequently occupied both a central and peripheral location (n = 79, 53%), and commonly presented with a solid appearance (n = 67, 44%) or with ground glass opacity (n = 80, 53%). Pleural effusions were seen in 68 patients (45%). Organizing pneumonia less frequently contained air bronchograms, cavitation, necrosis, surrounding ground glass opacity, or adjacent bronchiectasis. In patients with lung cancer (n = 25, 17%), OP more likely presented as discrete lesions and occupied a peripheral location as compared with patients with other malignancies (Ps = 0.025 and 0.002). In HSCT patients (n = 29, 19%), a diffuse process was more commonly seen than in non-HSCT patients (P = 0.038).

Conclusions
Organizing pneumonia more commonly presents as discrete lesions with a peripheral location in patients with lung cancer and as a diffuse process in patients who had undergone HSCT.
A novel mechanism of “metal gel-shift” by histidine-rich Ni2+-binding Hpn protein fromHelicobacter pyloristrain SS1
Authors: Mahadev Shelake Rahul , Ito Yuki , Masumoto Junya , Morita Eugene Hayato , Hayashi Hidenori 
Published: October 2016

AbstractSodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research. Migration of protein that does not correlate with formula MW, termed “gel shifting” appears to be common for histidine-rich proteins but not yet studied in detail. We investigated “gel shifting” in Ni2+-binding histidine-rich Hpn protein cloned fromHelicobacter pyloristrain SS1. Our data demonstrate two important factors determining “gel shifting” of Hpn, polyacrylamide-gel concentration and metal binding. Higher polyacrylamide-gel concentrations resulted in faster Hpn migration. Irrespective of polyacrylamide-gel concentration, preserved Hpn-Ni2+complex migrated faster (3-4 kDa) than apo-Hpn, phenomenon termed “metal gel-shift” demonstrating an intimate link between Ni2+binding and “gel shifting”. To examine this discrepancy, eluted samples from corresponding spots on SDS-gel were analyzed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). The MW of all samples was the same (6945.66±0.34 Da) and identical to formula MW with or without added mass of Ni2+. MALDI-TOF-MS of Ni2+-treated Hpn revealed that monomer bound up to six Ni2+ions non-cooperatively, and equilibrium between protein-metal species was reliant on Ni2+availability. This corroborates with gradually increased heterogeneity of apo-Hpn band followed by compact "metal-gel shift" band on SDS-PAGE. In view of presented data metal-binding and “metal-gel shift” models are discussed.
Tissue-aware RNA-Seq processing and normalization for heterogeneous and sparse data
Authors: Paulson Joseph N. , Chen Cho-Yi , Lopes-Ramos Camila M. , Kuijjer Marieke L , Platig John , Sonawane Abhijeet R. , Fagny Maud , Glass Kimberly , Quackenbush John 
Published: October 2016

AbstractAlthough ultrahigh-throughput RNA-Sequencing has become the dominant technology for genome-wide transcriptional profiling, the vast majority of RNA-Seq studies typically profile only tens of samples, and most analytical pipelines are optimized for these smaller studies. However, projects are generating ever-larger data sets comprising RNA-Seq data from hundreds or thousands of samples, often collected at multiple centers and from diverse tissues. These complex data sets present significant analytical challenges due to batch and tissue effects, but provide the opportunity to revisit the assumptions and methods that we use to preprocess, normalize, and filter RNA-Seq data – critical first steps for any subsequent analysis. We find analysis of large RNA-Seq data sets requires both careful quality control and that one account for sparsity due to the heterogeneity intrinsic in multi-group studies. An R package instantiating our method for large-scale RNA-Seq normalization and preprocessing, YARN, is available atbioconductor.org/packages/yarn.HighlightsOverview of assumptions used in preprocessing and normalizationPipeline for preprocessing, quality control, and normalization of large heterogeneous dataA Bioconductor package for the YARN pipeline and easy manipulation of count dataPreprocessed GTEx data set using the YARN pipeline available as a resource
Population diversity jeopardizes the efficacy of antibiotic cycling
Authors: Jiao Yunxin J. , Baym Michael , Veres Adrian , Kishony Roy 
Published: October 2016

AbstractTreatment strategies that anticipate and respond to the evolution of pathogens are promising tools for combating the global rise of antibiotic resistance1–3. Mutations conferring resistance to one drug can confer positive or negative cross-resistance to other drugs4. The sequential use of drugs exhibiting negative cross-resistance has been proposed to prevent or slow down the evolution of resistance5–8, although factors affecting its efficacy have not been investigated. Here we show that population diversity can disrupt the efficacy of negative cross-resistance-based therapies. By testing 3317 resistantStaphylococcus aureusmutants against multiple antibiotics, we show that first-step mutants exhibit diverse cross-resistance profiles: even when the majority of mutants show negative cross-resistance, rare positive cross-resistant mutants can appear. Using a drug pair showing reciprocal negative cross-resistance, we found that selection for resistance to the first drug in small populations can decrease resistance to the second drug, but identical selection conditions in large populations can increases resistance to the second drug through the appearance of rare positive cross-resistant mutants. We further find that, even with small populations and strong bottlenecks, resistance to both drugs can increase through sequential steps of negative cross-resistance cycling. Thus, low diversity is necessary but not sufficient for effective cycling therapies. While evolutionary interventions are promising tools for controlling antibiotic resistance, they can be sensitive to population diversity and the accessibility of evolutionary paths, and so must be carefully designed to avoid harmful outcomes.
Quantifying predator dependence in the functional response of generalist predators
Authors: Novak Mark , Wolf Christopher , Coblentz Kyle , Shepard Isaac 
Published: October 2016

AbstractA longstanding debate concerns whether functional responses are best described by prey-dependent versus ratio-dependent models. Theory suggests that ratio dependence can explain many food web patterns left unexplained by simple prey-dependent models. However, for logistical reasons, ratio dependence and predator dependence more generally have seen infrequent empirical evaluation and then only so in specialist predators, which are rare in nature. Here we develop an approach to simultaneously estimate the prey-specific attack rates and predator-specific interference rates of predators interacting with arbitrary numbers of prey and predator species. We apply the approach to field surveys and two field experiments involving two intertidal whelks and their full suite of potential prey. Our study provides strong evidence for the presence of weak predator dependence that is closer to being prey dependent than ratio dependent over manipulated and natural ranges of species abundances. It also indicates how, for generalist predators, even the qualitative nature of predator dependence can be prey-specific.Author contributionsCW contributed to method development, KC and IS performed the caging experiment, and MN conceived of the study, carried out the fieldwork and analyses, and wrote the manuscript.
Metabolomic and proteomic analyses of a quiescentEscherichia colicell factory reveal the mechanisms behind its production efficiency
Authors: Thomson Nicholas M. , Shirai Tomokazu , Chiapello Marco , Kondo Akihiko , Mukherjee Krishnan J. , Sivaniah Easan , Summers David K. , Numata Keiji 
Published: October 2016

1AbstractQuiescent (Q-Cell)Escherichia colicultures can be created by using the signalling molecule indole to halt cell division of anhnsmutant strain. This uncouples metabolism from cell growth and allows for more efficient use of carbon feedstocks. However, the reason for the increased productivity of cells in this state was previously unknown. We show here that Q-cells can maintain metabolic activity in the absence of growth for up to 24 h, leading to four times greater per-cell productivity of a model metabolite, 3-hydroxybutyrate (3HB), than a control. Metabolomic data show that by disrupting the proton-motive force, indole interrupts the tricarboxylic acid cycle, leading to the accumulation of metabolites in the glycolysis pathway that are excellent starting points for high-value chemical production. By comparing protein expression patterns between wild-type and Q-cell cultures we show that Q-cells overexpress stress response proteins, which prime them to tolerate the metabolic imbalances incurred through indole addition. Quiescent cultures produced half the cell biomass of control cultures lacking indole, but were still able to produce 39.4 g.L-1of 3HB compared to 18.6 g.L-1in the control. Therefore, Q-cells have high potential as a platform technology for the efficient production of a wide range of commodity and high value chemicals.
The producer-consumer classification gap and its effects on music festival success
Authors: Kuijken Bram , Leenders Mark A.A.M. , Wijnberg Nachoem M. , Gemser Gerda 
Published: October 2016

Purpose
Producers and consumers – who represent opposing sides of the market – have different frames of reference, which may result in differences in classification of the same products. The authors aim to demonstrate that “classification gaps” have a negative effect on the performance of products and that these effects play a role in different stages of consumers’ decision process.

Design/methodology/approach
The data collection consisted of three comprehensive parts covering production and consumption in the music festival market in The Netherlands. The first part focused on festival organizers who were asked to classify their own music festival in terms of musical genres. In total, 70 festival organizers agreed to participate. The second part measured the genre classification of 540 consumers. In the third part, the authors interviewed 1,554 potential visitors of music festivals in The Netherlands about their awareness of the festival and if they considered visiting or actually visited the festival.

Findings
This paper provides empirical evidence that a classification gap between the production side and the consumption side of the market has negative effects on music festival performance. In addition, the authors found that this is in part because of lower activation of potential consumers in the marketplace.

Practical implications
An important practical implication of this study is that – in general – producers should be aware that classification gaps can occur – even if they are sure about the classification of their products – and that this can have serious consequences. The category membership of products is often seen as a given, whereas it cannot be assumed that the classification perceived by different economic groups is the same – as demonstrated in this paper.

Originality/value
This paper demonstrates that a fundamental – but understudied – disconnect between the two opposing sides of the market (i.e. producers and consumers) regarding the classification of the same products can have negative effects on performance of these products.
Understanding the selfie phenomenon: current insights and future research directions
Authors: Lim Weng Marc 
Published: October 2016

PurposeThis paper aims to define the conceptual boundary of the selfie and to discuss the role of the selfie in the social media marketplace.Design/methodology/approachThis paper extensively reviews and draws themes from the extant literature on consumer identities in the social media marketplace to explain the selfie phenomenon and to identify potentially fruitful directions for further research.FindingsCurrent insights into the selfie phenomenon can be understood from socio-historical, technological, social media, marketing and ethical perspectives.Research limitations/implicationsDespite the limitations of a general review (e.g. absence of empirical data and analysis), this paper identifies multiple avenues to extend existing lines of inquiry on the selfie phenomenon. Thus, this paper should encourage further research on the topic in the academic and scientific community.Practical implicationsThe selfie can be used as a marketing tool to improve marketing performance and accomplish marketing-related goals.Originality/valueThis paper sheds light on how marketing academics and practitioners can better understand the impact of the selfie in the social media marketplace.
Brand selfies: consumer experiences and marketplace conversations
Authors: Presi Caterina , Maehle Natalia , Kleppe Ingeborg Astrid 
Published: October 2016

Purpose
The purpose of this paper is to explore the brand selfie phenomenon on two different levels. On the level of consumer brand experiences, the focus is on how brand selfie practices add new features to brand experiences and consumer–brand relationships. On the level of marketplace brand image, the authors explore how consumers contribute to marketplace conversations by posting brand selfies in social media and how this practice shapes and changes brand image.

Design/methodology/approach
The researchers conducted an extensive search on different social media platforms to collect various types of brand selfies. The authors approach brand selfies as rich visual texts and their analysis comprises four key steps: descriptive analysis, response analysis, formal analysis and polytextual thematic analysis.

Findings
On the level of consumer brand experiences, the findings illuminate how different types of brand selfies extend the brand experience in space and time and transfer it into the hybrid space of the consumer-defined social networks. On the level of marketplace brand image, it is illustrated how brand selfies contribute to the process of co-creating brand meaning in the social media.

Originality/value
The study proposes a typology of brand selfie assemblages showing how consumers contribute to the visual production and consumption of brand meanings. The brand selfie is a unique material and expressive reality enabling us to theorise new perspectives on how consumers consume brands and how aggregates of brand selfie production and dissemination affect marketplace dynamics.
Insight into the motivation of selfie postings: impression management and self-esteem
Authors: Pounders Kathrynn , Kowalczyk Christine M. , Stowers Kirsten 
Published: October 2016

Purpose
Social media enables consumers to regularly express themselves in a variety of ways. Selfie-postings are the new tool for self-presentation, particularly among millennials. The purpose of this paper is to identify the motivations associated with selfie-postings among female millennials.

Design/methodology/approach
The exploratory study consisted of 15 in-depth interviews with women who were 19-30 years of age. The analysis of data was facilitated by an iterative constant comparison method between data, emerging concepts and extant literature.

Findings
Textual analysis reveals impression management to be pivotal in understanding the consumer selfie-posting process. Other sub-themes include happiness and physical appearance. In addition, self-esteem was revealed as a motivator and an outcome.

Research limitations/implications
The study was limited to females who were 19-30 years of age. Future research should include males and a wider age group and focus on empirical testing of the identified themes.

Practical implications
This research sheds light on the motivation and outcomes associated with selfie-postings. Implications for marketers and advertisers include a better understanding of how to engage consumers to post content in the form of selfies with brands and products.

Originality/value
This paper fulfils an identified need to explore the growing trend of selfie-postings and contributes to academic literature in consumer behavior by identifying the motivations of selfie-postings.