bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-Genome Sequences of Zika Virus FLR Strains after Passage in Vero or C6/36 Cells
Authors: Moser Lindsey A. , Oldfield Lauren M. , Fedorova Nadia , Puri Vinita , Shrivastava Susmita , Amedeo Paolo , Isom Richard , Hu Lihui , Durbin Alan , Rocchi Iara , Williams Torrey , Tan Gene S. , Shabman Reed S. , Bernard Kristen A. , Pickett Brett E. 
Published: January 2018

ABSTRACT
We report 26 complete genomes of Zika virus (ZIKV) isolated after passaging the Zika virus strain FLR in mosquito (C6/36) and mammalian (Vero) cell lines. The consensus ZIKV genomes we recovered show greater than 99% nucleotide identify with each other and with the FLR strain used as input.
Complete Genome Sequence of
Bacillus velezensis
CN026 Exhibiting Antagonistic Activity against Gram-Negative Foodborne Pathogens
Authors: Nannan Catherine , Gillis Annika , Caulier Simon , Mahillon Jacques 
Published: January 2018

ABSTRACT

We report here the complete genome sequence of
Bacillus velezensis
strain CN026, a member of the
B. subtilis
group, which is known for its many industrial applications. The genome contains 3,995,812 bp and displays six gene clusters potentially involved in strain CN026’s activity against Gram-negative foodborne pathogens.
Draft Genome Sequences of Four
Salmonella enterica
subsp.
enterica
Serovar Enteritidis Strains Implicated in Infections of Avian and Human Hosts
Authors: An Ran , Lin Pengpeng , Bougouffa Salim , Essack Magbubah , Boxrud David , Bajic Vladimir B. , Vidovic Sinisa 
Published: January 2018

ABSTRACT

Salmonella enterica
subsp.
enterica
serovar Enteritidis is a wide-host-range pathogen. Occasionally, it is involved in invasive infections, leading to a high mortality rate. Here, we present the draft genome sequences of four
S
. Enteritidis strains obtained from human and avian hosts that had been involved in bacteremia, gastroenteritis, and primary infections.
Complete Genome Sequence of an
Escherichia coli
O121:H19 Strain from an Outbreak in Canada Associated with Flour
Authors: Robertson James , Lin Janet , Levett Paul N. , Nadon Celine , Nash John , Berry Chrystal 
Published: January 2018

ABSTRACT

Here, we present the first complete genome sequence of an
Escherichia coli
non-O157 Shiga-toxin producing isolate, 16-9255, from serotype O121:H19. This strain is notable as a clinical case recovered from a recent Canadian flour-associated outbreak event.
Complete Genome Sequence of
Mycobacterium
sp. Strain 3519A
Authors: Bouam Amar , Levasseur Anthony , Bonnet Maryline , Borand Laurence , Van Goethem Charles , Drancourt Michel , Godreuil Sylvain 
Published: January 2018

ABSTRACT

Mycobacterium
sp. strain 3519A is a nontuberculous mycobacterium isolated from sputum from a Cambodian patient with a pulmonary infection. We report here the first complete 7.3-Mbp-long genome sequence of
Mycobacterium
sp. 3519A with 66.35% GC content, encoding 7,029 protein-coding genes, 50 tRNAs, and 5 rRNA genes.
Draft Genome Sequences of Two Psychrotolerant Strains,
Colwellia polaris
MCCC 1C00015
T
and
Colwellia chukchiensis
CGMCC 1.9127
T
Authors: Zhang Cong , Guo Wenbin , Wang Yuguang , Chen Xinhua 
Published: January 2018

ABSTRACT

Colwellia polaris
MCCC 1C00015
T
and
Colwellia chukchiensis
CGMCC 1.9127
T
are psychrotolerant bacteria isolated from the Canadian Basin and Chukchi Sea, respectively. Here, we report the draft genome sequences of
C. polaris
MCCC 1C00015
T
and
C. chukchiensis
CGMCC 1.9127
T
, which will help reveal how they adapt to cold environments.
Mimicry of Cellular A Kinase-Anchoring Proteins Is a Conserved and Critical Function of E1A across Various Human Adenovirus Species
Authors: King Cason R. , Gameiro Steven F. , Tessier Tanner M. , Zhang Ali , Mymryk Joe S. 
Published: January 2018

ABSTRACT
The E1A proteins of the various human adenovirus (HAdV) species perform the critical task of converting an infected cell into a setting primed for virus replication. While E1A proteins differ in both sequence and mechanism, the evolutionary pressure on viruses with limited coding capacity ensures that these proteins often have significant overlap in critical functions. HAdV-5 E1A is known to use mimicry to rewire cyclic AMP (cAMP) signaling by decoupling protein kinase A (PKA) from cellular A kinase-anchoring proteins (AKAPs) and utilizing PKA to its own advantage. We show here that E1As from other species of HAdV also possess this viral AKAP (vAKAP) function and examine how they manipulate PKA. E1A from most species of HAdV examined contain a small AKAP-like motif in their N terminus which targets the docking-dimerization domain of PKA as the binding interface for a conserved protein-protein interaction. This motif is also responsible for an E1A-mediated relocalization of PKA regulatory subunits from the cytoplasm into the nucleus, with species-specific E1A proteins having preference for one particular isoform of PKA subunit over another. Importantly, we showed that these newly characterized vAKAPs can integrate into cAMP-responsive transcription as well as contribute to viral genome replication and infectious progeny production for several distinct HAdV species.

IMPORTANCE
These data enhance the mechanistic knowledge on how HAdV E1A manipulates cellular PKA to benefit infection. The work establishes that mimicry of AKAPs and subversion of PKA-mediated cAMP signaling are conserved features for numerous human adenoviruses. This study also highlights the molecular determinants conferring selective protein-protein interactions between distinct PKA regulatory subunits and the different E1A proteins of these viruses. Additionally, it further emphasizes the utility of using viral proteins like E1A as tools for studying the molecular biology of cellular regulatory pathways.
Pre–mass extinction decline of latest Permian ammonoids
Authors: Kiessling Wolfgang , Schobben Martin , Ghaderi Abbas , Hairapetian Vachik , Leda Lucyna , Korn Dieter 
Published: January 2018

Abstract
The devastating end-Permian mass extinction is widely considered to have been caused by large-scale and rapid greenhouse gas release by Siberian magmatism. Although the proximate extinction mechanisms are disputed, there is widespread agreement that a major extinction pulse occurred immediately below the biostratigraphically defined Permian–Triassic boundary. Our statistical analyses of stratigraphic confidence intervals do not comply with a single end-Permian extinction pulse of ammonoids in Iran. High turnover rates and extinction pulses are observed over the last 700 k.y. of the Permian period in two widely separated sections representative of a larger area. Analyses of body sizes and morphological complexity support a gradual decline over the same interval. Similar pre–mass extinction declines and disturbances of the carbon cycle have sometimes been reported from other regions, suggesting a widespread, but often overlooked, environmental deterioration at a global scale, well before the traditional main extinction pulse.
The signature of undetected change: an exploratory electrotomographic investigation of gradual change blindness
Authors: Kiat John E. , Dodd Michael D. , Belli Robert F. , Cheadle Jacob E. 
Published: January 2018

Neuroimaging-based investigations of change blindness, a phenomenon in which seemingly obvious changes in visual scenes fail to be detected, have significantly advanced our understanding of visual awareness. The vast majority of prior investigations, however, utilize paradigms involving visual disruptions (e.g., intervening blank screens, saccadic movements, “mudsplashes”), making it difficult to isolate neural responses toward visual changes cleanly. To address this issue in this present study, high-density EEG data (256 channel) were collected from 25 participants using a paradigm in which visual changes were progressively introduced into detailed real-world scenes without the use of visual disruption. Oscillatory activity associated with undetected changes was contrasted with activity linked to their absence using standardized low-resolution brain electromagnetic tomography (sLORETA). Although an insufficient number of detections were present to allow for analysis of actual change detection, increased beta-2 activity in the right inferior parietal lobule (rIPL), a region repeatedly associated with change blindness in disruption paradigms, followed by increased theta activity in the right superior temporal gyrus (rSTG) was noted in undetected visual change responses relative to the absence of change. We propose the rIPL beta-2 activity to be associated with orienting attention toward visual changes, with the subsequent rise in rSTG theta activity being potentially linked with updating preconscious perceptual memory representations.NEW & NOTEWORTHY This study represents the first neuroimaging-based investigation of gradual change blindness, a visual phenomenon that has significant potential to shed light on the processes underlying visual detection and conscious perception. The use of gradual change materials is reflective of real-world visual phenomena and allows for cleaner isolation of signals associated with the neural registration of change relative to the use of abrupt change transients.
Fc-Mediated Anomalous Biodistribution of Therapeutic Antibodies in Immunodeficient Mouse Models
Authors: Sharma Sai Kiran , Chow Andrew , Monette Sebastien , Vivier Delphine , Pourat Jacob , Edwards Kimberly J. , Dilling Thomas R. , Abdel-Atti Dalya , Zeglis Brian M. , Poirier John T. , Lewis Jason S. 
Published: January 2018

Abstract
A critical benchmark in the development of antibody-based therapeutics is demonstration of efficacy in preclinical mouse models of human disease, many of which rely on immunodeficient mice. However, relatively little is known about how the biology of various immunodeficient strains impacts the in vivo fate of these drugs. Here we used immunoPET radiotracers prepared from humanized, chimeric, and murine mAbs against four therapeutic oncologic targets to interrogate their biodistribution in four different strains of immunodeficient mice bearing lung, prostate, and ovarian cancer xenografts. The immunodeficiency status of the mouse host as well as both the biological origin and glycosylation of the antibody contributed significantly to the anomalous biodistribution of therapeutic monoclonal antibodies in an Fc receptor-dependent manner. These findings may have important implications for the preclinical evaluation of Fc-containing therapeutics and highlight a clear need for biodistribution studies in the early stages of antibody drug development.
Significance: Fc/FcγR-mediated immunobiology of the experimental host is a key determinant to preclinical in vivo tumor targeting and efficacy of therapeutic antibodies. Cancer Res; 78(7); 1820–32. ©2018 AACR.
NK Cells Control Tumor-Promoting Function of Neutrophils in Mice
Authors: Ogura Keisuke , Sato-Matsushita Marimo , Yamamoto Seiji , Hori Takashi , Sasahara Masakiyo , Iwakura Yoichiro , Saiki Ikuo , Tahara Hideaki , Hayakawa Yoshihiro 
Published: January 2018

Abstract
Although natural killer (NK) cells are recognized as direct antitumor effectors, the ability of NK cells to control cancer-associated inflammation, which facilitates tumor progression, remains unknown. In this study, we demonstrate that NK cells control tumor-promoting inflammation through functional modification of neutrophils. NK cells control the tumor-promoting function of neutrophils through an IFNγ-dependent mechanism. Tumor progression in an NK cell–depleted host is diminished when the IL17A–neutrophil axis is absent. In NK cell–depleted mice, neutrophils acquire a tumor-promoting phenotype, characterized by upregulation of VEGF-A expression, which promotes tumor growth and angiogenesis. A VEGFR inhibitor which preferentially suppressed tumor growth in NK cell–depleted mice was dependent on neutrophils. Furthermore, the systemic neutropenia caused by an antimetabolite treatment showed an anticancer effect only in mice lacking NK cells. Thus, NK cells likely control the tumor-promoting and angiogenic function of neutrophils. Cancer Immunol Res; 6(3); 348–57. ©2018 AACR.
Stabilization of Olecranon Fractures by Tension Band Wiring or Plate Osteosynthesis: A Retrospective Study of 41 Cases
Authors: Boursier Jean-François , Corbeau Solène , Decambron Adeline , Viateau Véronique , Fayolle Pascal , Bedu Anne-Sophie , Leperlier Dimitri , Manassero Mathieu , Fournet Alexandre 
Published: January 2018

Objectives This article aimed to describe olecranon fracture in dogs and cats and their stabilization with tension band wiring or plate osteosynthesis, and to evaluate complications associated with each technique.
Methods Medical records of cats and dogs that had been surgically treated for olecranon fractures with either tension band wiring or plate osteosynthesis were retrospectively reviewed. The surgical technique, complications and long-term outcomes were assessed.
Results Forty-one olecranon fractures were included. Fractures were articular, comminuted and open in 90, 31 and 27% of cases, respectively. Tension band wiring and plate osteosynthesis were performed in 22 and 19 fractures, respectively. Complications occurred more commonly after tension band wiring (74%) compared with plate osteosynthesis (27%) (p = 0.002) and these were probably related to it being used in comminuted fractures (p = 0.01) or to errors in technique. Minor complications included Kirschner wires migration (n = 5), pain (n = 3), osteomyelitis (n = 3), skin breakdown (n = 3) and seroma (n = 1). Implant failure requiring further fixation (n = 4) was observed only in the tension band wiring group. Other major complications included skin wound debridement and closure (n = 1) and chronic lameness requiring implant removal (n = 7). Long-term functional outcomes were excellent regardless of the technique used.
Clinical Significance Plate osteosynthesis should be performed for olecranon fracture repair if technically feasible.
Accuracy of Prediction Instruments for Diagnosing Large Vessel Occlusion in Individuals With Suspected Stroke: A Systematic Review for the 2018 Guidelines for the Early Management of Patients With Acute Ischemic Stroke
Authors: Smith Eric E. , Kent David M. , Bulsara Ketan R. , Leung Lester Y. , Lichtman Judith H. , Reeves Mathew J. , Towfighi Amytis , Whiteley William N. , Zahuranec Darin B. 
Published: January 2018

Introduction—
Endovascular thrombectomy is a highly efficacious treatment for large vessel occlusion (LVO). LVO prediction instruments, based on stroke signs and symptoms, have been proposed to identify stroke patients with LVO for rapid transport to endovascular thrombectomy–capable hospitals. This evidence review committee was commissioned by the American Heart Association/American Stroke Association to systematically review evidence for the accuracy of LVO prediction instruments.

Methods—
Medline, Embase, and Cochrane databases were searched on October 27, 2016. Study quality was assessed with the Quality Assessment of Diagnostic Accuracy-2 tool.

Results—
Thirty-six relevant studies were identified. Most studies (21 of 36) recruited patients with ischemic stroke, with few studies in the prehospital setting (4 of 36) and in populations that included hemorrhagic stroke or stroke mimics (12 of 36). The most frequently studied prediction instrument was the National Institutes of Health Stroke Scale. Most studies had either some risk of bias or unclear risk of bias. Reported discrimination of LVO mostly ranged from 0.70 to 0.85, as measured by the C statistic. In meta-analysis, sensitivity was as high as 87% and specificity was as high as 90%, but no threshold on any instruments predicted LVO with both high sensitivity and specificity. With a positive LVO prediction test, the probability of LVO could be 50% to 60% (depending on the LVO prevalence in the population), but the probability of LVO with a negative test could still be ≥10%.

Conclusions—
No scale predicted LVO with both high sensitivity and high specificity. Systems that use LVO prediction instruments for triage will miss some patients with LVO and milder stroke. More prospective studies are needed to assess the accuracy of LVO prediction instruments in the prehospital setting in all patients with suspected stroke, including patients with hemorrhagic stroke and stroke mimics.
Who Speaks for China?
Authors: DeHart Monica 
Published: January 2018

AbstractDrawing on ethnographic analysis of a Confucius Institute and two private schools, this article analyzes how diverse Chinese language institutes in Costa Rica have sought to capitalize on a growing local interest in learning Mandarin Chinese. It argues that a shifting global geopolitics has increased the perceived value of Chinese language acquisition and, thus, the stakes for language institutes seeking to assert their cultural authority as legitimate purveyors of Chinese and Chineseness. Through analysis of these schools’ projected identities and pedagogical styles, I show how they distinguish themselves from one another on the basis of public versus private ownership, choice-based versus authoritarian instructional style, and Taiwanese versus Mainland or diasporic roots. Building on the concept of the “Sinophone,” I highlight both the diversity of the forms and locations of Chineseness these initiatives represent and their implications for who can legitimately speak for China in Costa Rica.
Contested Encounters
Authors: Harris Karen L. 
Published: January 2018

AbstractThis article focuses on China’s initial encounter with the African continent from the perspective of a select literature overview. It reflects on the very earliest contacts between dynastic China and ancient Africa and shows that the current contestation in the Western media as well as literature over this more recent contact is not new. Given the dearth and disparate nature of the information on these first encounters, it does this through the lens of what has been written on the subject of the speculated first contact in a selection of secondary English-language literature. It does so by considering the prevalence of such literature in three distinct periods: prior to 1949; from 1950 to 1990; and a selection of research published thereafter. It shows that China’s encounter with Africa reaches far back into the history of the continent, but more importantly so does the volatile contestation surrounding the contemporary contact.
The Silver Triton
Authors: Dorin Alan , Anagnostou-Laoutides Eva 
Published: January 2018

Abstract
This article assesses the imperial Roman technological options and cultural impetus for constructing and displaying an automaton Triton. Suetonius reports that such a figure announced the commencement of a staged sea battle organised by Emperor Claudius to entertain the Roman citizens in 52 CE. This automaton, whose feasibility we assess, fits neatly as an application of the pneumatic techniques summarised by Heron of Alexandria, who was probably alive at the time. By drawing attention to this little discussed passage of Suetonius, our article corroborates the idea that these techniques were useful – here contributing to the “media-image” and audio-visual culture of Claudius’ imperial agenda – and that their wondrous effects provided an intellectual bridge between their practical utility and their ability to contribute to the philosophy of science and technology.
The Light of the Emotions
Authors: Giacomoni Paola 
Published: January 2018

Abstract
The study of emotions is one of the most interesting, and interdisciplinary topic in contemporary international research, but it is rarely supported by a substantial historical background. This essay aims at reconstructing one of the most significant turning points in the field of affective studies in the seventeenth Century French culture. René Descartes’ last work, Les Passions de l’ âme, proposes an innovative, scientific approach to the study of emotions and their expression, based on a new theory of perception and light. The role played by the visible expression of passions in Descartes’ treatise inaugurates a new and fruitful path of research. Furthermore, the essay analyzes the writings by the painter Charles Le Brun, who proposes a visual interpretation of the expression of emotions, and some works by the physician Marin Cureau de la Chambre, who interprets the passions and their expression from a medical and optical perspective.
High-Resolution Historical Climate Simulations over Alaska
Authors: Monaghan Andrew J. , Clark Martyn P. , Barlage Michael P. , Newman Andrew J. , Xue Lulin , Arnold Jeffrey R. , Rasmussen Roy M. 
Published: January 2018

AbstractWeather and climate variability strongly influence the people, infrastructure, and economy of Alaska. However, the sparse observational network in Alaska limits our understanding of meteorological variability, particularly of precipitation processes that influence the hydrologic cycle. Here, a new 14-yr (September 2002–August 2016) dataset for Alaska with 4-km grid spacing is described and evaluated. The dataset, generated with the Weather Research and Forecasting (WRF) Model, is useful for gaining insight into meteorological and hydrologic processes, and provides a baseline against which to measure future environmental change. The WRF fields are evaluated at annual, seasonal, and daily time scales against observation-based gridded and station records of 2-m air temperature, precipitation, and snowfall. Pattern correlations between annual mean WRF and observation-based gridded fields are r = 0.89 for 2-m temperature, r = 0.75 for precipitation, r = 0.82 for snow-day fraction, r = 0.55 for first snow day of the season, and r = 0.71 for last snow day of the season. A shortcoming of the WRF dataset is that spring snowmelt occurs too early over a majority of the state, due partly to positive 2-m temperature biases in winter and spring. Strengths include an improved representation of the interannual variability of 2-m temperature and precipitation and accurately simulated (relative to regional station observations) winter and summer precipitation maxima. This initial evaluation suggests that the 4-km WRF climate dataset robustly simulates meteorological processes and recent climatic variability in Alaska. The dataset may be particularly useful for applications that require high-temporal-frequency weather fields, such as driving hydrologic or glacier models. Future studies will provide further insight on its ability to represent other aspects of Alaska’s climate.
A Novel Instrument for Real-Time Measurement of Attenuation of Weather Radar Radome Including Its Outer Surface. Part I: The Concept
Authors: Mancini Alessio , Salazar Jorge L. , Lebrón Rodrigo M. , Cheong Boon Leng 
Published: January 2018

AbstractThis paper presents a unique instrument for characterizing the impact of wet radomes in radar systems. The proposed technique enables full radio frequency (RF) characterization of the radome, by evaluating its performance under a variety of conditions, including dirtiness, wetness, ice formation, and varying temperature, and providing a potential solution for future wet radome calibration methods. The reflections generated from the wet radome surface, measured from a high-resolution probe, are combined with an estimation of water absorption as a function of the precipitation rain rate, to calculate the attenuation introduced by the wet radome. This instrument is a cost-effective solution that can be integrated into an existing or new radar system.