bluuebunny/crossref_metadata_2025_split huggingface dataset:
ISOLASI DAN IDENTIFIKASI SENYAWA KIMIA SERTA UJI AKTIVITAS ANTICANDIDAISIS SERBUK BATANG SIRIH DUDUK (Piper sarmentosum Roxb. Ex Hunter)
Authors: Suwandri Suwandri , Diastuti Hartiwi , Purwati Purwati 
Published: February 2017

Sirih duduk or Piper sarmentosum Roxb. ex Hunter have been long used the people in Indonesia for traditional medicine to cure various diseases. One of them was used to treat fungoid diseases. This research was aimed to examine the anticandidaisis activity from extract of P. sarmentosum bark, isolate and identify anticandidaisis compounds from P. sarmentosum bark. The isolation of bioactive compounds of P. sarmentosum bark was performed by extraction the powder of P. sarmentosum bark using methanol. The methanol extract was then fractionated using n-hexane, chloroform and ethylasetat. The fractions respectively was examined their activity against C. albican. The active extract was then fractionated performed by coloumn chromatography and was then separated performed by thin layer chromatography. Identification of bioactive compound was carry out using gas chromatography-mass spectrometry (GC-MS). The results show that chloroform, ethylasetat and methanol extracts of P. sarmentosum have more activities against C. albicans than miconazol. Bioactive compound from extract of P. sarmentosum bark was bis(2-ethylhexyl)phtalat.
PENURUNAN KADAR ZAT WARNA RODAMIN B MENGGUNAKAN HUMIN HASIL ISOLASI DARI TANAH HUTAN DAMAR BATURRADEN
Authors: Setyaningtyas Tien , Andreas Roy 
Published: February 2017

Humin was isolated from the Baturraden resin forest and purified use mixture of HCl:HF. Purified humin is characterised such as identify of functional group of humin, stipulating of water content, stipulating of dust content, obstetrical stipulating of total acidity content, carboxylic and OH phenolic functional group. The adsorption of rodamin by humin measured by UV-Visible spectrophotometer at maximum wavelength of rodamin B liquid, optimum pH and optimum contact time to determine absorbtion capacities and isotherm adsorption pattern .Humin that isolated from Baturraden resin forest have characteristic such as water content 16.6199 %; dust content 9.2050 %; total acidity content 475 cmol / Kg; carboxylic rate 200 cmol/Kg, and OH phenolic rate 275 cmol/Kg. Maximum wavelength of rodamin B is 554 nm and optimum contact time of adsorption rodamin B by humin is 180 minute with optimum pH 4. Adsorption rodamin B by humin follows the pattern of Langmuir isotherm adsorptionwith the value of R2 is 0.9801 and absorption capacities is 39,5257 mg/g.
PREPARASI DAN KARAKTERISASI KITIN DARI KULIT UDANG PUTIH (Litophenaeus vannamei)
Authors: Kurniasih Mardiyah , Windy Dwiasi Dian 
Published: February 2017

Chitin is one of the most abundant natural polysaccharides produced by many living organisms; it is usually found as a component of crustacean shells. In this paper, Chitin have been isolated from white shrimp (Litophenaeus vannamei). The preparation of chitin using chemical products to deproteinize and demineralize the source material. Characterization included determination of water, ash, fat and protein degree; moreover chitin powder characterize with FTIR and XRD spectroscopy. The result showed that process efficiency of chitin from white shrimp (Litophenaeus vannamei) was 20.95%, with degree of water, ash, fat and protein were 5.39, 2.66, 1.54 and 36.16%, respectively.
PENGOLAHAN LIMBAH LOGAM BERAT INDUSTRI TEKSTIL MENGGUNAKAN FOTOKATALIS TiO2/ARANG AKTIF
Authors: Riyani Kapti , Setyaningtyas Tien , Andreas Roy 
Published: February 2017

Study about the use of active charcoal rice straws (AAJP) was done in order to decrease heavy metal ion on textile waste water by using photocatalyst AAJP/TiO2. Rice straws were gained from the Banyumas Regency and samples textile waste water from one of textile industry in Pemalang. This study was begun by the making of active charcoal from the rice straws, and then the modification of photocatalyst AAJP/TiO2 was done with the comparison 1:99, 2:98, 3:97, 4:96, 5:95. The test of heavy metal ion photoreduction was done with some parameters such as weight comparison (1:99, 2:98, 3:97, 4:96, 5:95), pH effect (2, 5, 7, 9, 13) and the effect of ray source (sun light and the UV lamp). Analysis of photocatalyst material used SEM and heavy metal ion concentration used AAS. Results show that optimum photocatalyst activity in weight comparison AAJP300oC / TiO2 (1:99) with decrease concentration was 65.02% for heavy metal ion, mean while AAJP700oC / TiO2 (3:97) with decreased concentration of heavy metal ion is 94.50%. Optimal process for heavy metal ion reduction occur at pH 2 with decreased concentration of heavy metal ion for AAJP300oC / TiO2 (1:99) is 99.87% and for AAJP700oC /TiO2 (3:97) is 100%. The source of UV lamp was good for heavy metal ion photoreduction (AAJP300oC/TiO2(1:99) was 99.87% and AAJP700oC/TiO2 (3:97) was 100%)
PENUMBUHAN DAN KARAKTERISASI LAPISAN TIPIS PbTiO3 YANG DISIAPKAN DENGAN TEKNIK SPIN COATING
Authors: Bilalodin Bilalodin 
Published: February 2017

The growth of PbTiO3 ferroelectric thin films have successfully done. Thin films were made from bulk (powder) PbTiO3 dissolved in methanol solution. The condensation was mixed during 1 hour to get homogeneous condensation. Thin films were grown above corning substrates by spin coating method. Optimation was done by various of annealing temperature. The physical properties of thin films were characterized by Energi Dispersive X-Ray Spectroscopy (EDS), X-Ray Diffraction (XRD), Scanning and Electron Microscopy (SEM). EDS measurement showed that the stoichiometry composition ratio of Pb/Ti is 1/1.26 at annealing temperature 600oC and 1/1.29 at annealing temperature 700oC. The result of XRD pattern showed that crystal structure of PbTiO3 thin films are tetragonal. The calculated lattice parameters ontained from Chohen Method are a=b= 3.873 Å dan c= 4.130Å. The result of SEM PbTiO3 thin film showed that thin film has globular grain size.
FRAKSINASI DAN IDENTIFIKASI SENYAWA ANTIKANKER EKSTRAK KULIT BATANG Rhizopora mucronata SERTA UJI TOKSISITASNYA TERHADAP LARVA UDANG (Artemia salina Leach)
Authors: Diastuti Hartiwi , Suwandri Suwandri 
Published: February 2017

Investigation the anticancer potency of R. mucronata has not been carried yet. This research were aimed to extract the bioactive compound of R. mucronata with various organic solvents, examine their toxicity againts A. salina Leach larv. and identify the toxic compounds from R. mucronata steam bark.The extraction of R. mucronata steam bark were peformed by maseration with n-hexane, chloroform, ethylacetate and methanol, repectively. The extracts were examined their toxicity againsts A. salina Leach larv. The highest activity extracts was fractionated performed by coloumn chromatography. The fractions respectively was examined their toxicity againts A. salina Leach larv. Identification of toxic compound was carry out using gas chromatography-mass spectrometry (GC-MS).The chloroform extracts of R. mucronata had toxic character. Toxicity test to A. salina Leach larv. The Result showed that the chloroform fraction 1 (C1) of R. mucronata steam bark had LC50 equal to 301.50 ppm. Phytochemical study showed that the active fractions contained terpenoid. Bioactive compound from R. mucronatasteam bark were dioctyl phthalate and cyclopropyle azulene decahydro-1,1,7-trimethyl-4-methylene.
SIFAT-SIFAT BIOKIMIAWI EKSTRAK KASAR LIPASE EKSTRASELULER DARI BAKTERI Azospirillum sp. JG3
Authors: Lestari Puji , Nur Handayani Santi , Oedjijono Oedjijono 
Published: February 2017

Lipases are valuable biocatalysts because they act under extremely mild conditions, are stable in organic solvents, show broad substrate specificity and exhibit high stereoselectivity. Lipases play important role in various industries such as detergent, cosmetics, flavor, pharmacy and synthesis of organic compounds. The increasing of lipases requirements in industries is goading research to get new lipases resources commited. One of potential lipase resource is Azospirillum sp.JG3 bacteria from Microbiology Laboratory of Biology Faculty University of Jenderal Soedirman. The specific targets of this research are to get crude extract of lipase and investigate its biochemical characteristics. The method used were rejuvenation of Azospirillum sp.JG3 bacteria, inoculum production, determination of optimum production time and bacterium growth phase, extraction and production of lipase to get crude extract, and characterization the biochemical properties of lipase crude extract. The research resulted that crude extract of lipase from Azospirillum sp.JG3 had optimum temperature at 40 °C and optimum pH at pH 7. The lipase was a metalloenzyme with Ca2+ as its cofactor. The lipase was stable in three organic solvents tested, (chloroform, n-hexane and ether).
KECEPATAN ADUK DAN WAKTU KONTAK OPTIMUM PEMBUATAN BIODIESEL DARI MINYAK JELANTAH
Authors: Kartika Dwi , Vaulina Eva , Widyaningsih Senny , Chasani Moch. 
Published: February 2017

Synthesis of biodiesel from waste cooking oil using activated natural zeolite catalyst has been done. Activation of the natural zeolite was done by refluxing with HCl 6M for 30 min, calcining and oxydizing at 500°C for 2 hours, consecutively. The variation of stirring speed were 350, 700, 1100 and 1200 rpm. The variation of reaction time were varied from 15, 30, 45, 60, and 120 min. The conversion of biodiesel was determined by 1H NMR spectrometer. The results showed that the optimum condition of biodiesel synthesis using esterification process were reached at 700 rpm and 15 minutes, which gave biodiesel conversion of 100%.
OPTIMASI BIOKRAFT JAMUR Phanerochaete chrysosporium TERHADAP KOMPONEN KIMIA CAMPURAN BATANG DAN LIMBAH CABANG MANGIUM SEBAGAI BAHAN BAKU PULP
Authors: Silsia Devi , Yahya Ridwan , Mucharomah Mucharomah 
Published: February 2017

Optimation biokraft of fungi P. Chrysosporium through elongated incubation time on mixed stem and branch waste mangium is a solution to solve the environmental pollution problem, low quality of pulp and limited raw material. Effect of P. Chrysosporium 10 % concentration and 45 days incubation time on pre research could not decrease lignin optimally and exstractive degradation had not occured yet. The aims of the study were to observe the effect of incubation time extension, and to determine the best incubation time of P. Chrysosporium applied at 10 % concentration based on the chemical component percentage, 45, 60 and 75 days on mixed stem and branch as raw material for pulp. Results showed that increasing incubation time decreased extractive and lignin content and increased holocelulosa and alpha celulosa content. Mixed stem and branch with 10% amount and 75 day incubation time of P. Chrysosporium gave the best results for raw material of pulp.
PEMANFAATAN KATALIS NI/ZEOLIT PADA HIDROGENASI KATALITIK ETIL PALMITAT MENJADI SETIL ALKOHOL
Authors: Kartika Dwi , Triyono Triyono , Wijaya Karna , Yuanita L Dewi , D. Setyawan PH D. Setyawan PH 
Published: February 2017

The catalytic hydrogenation of methyl palmitate to cetyl alcohol using Ni supported on activated natural zeolite catalysts (Ni/Zeolite) has been carried out. In this work, the effect of catalyst amounts and H2 flow rate on methyl palmitate conversion and yield of cetyl alcohol were studied. Catalytic hydrogenation was performed in stainless steel fixed bed reactor. The methyl palmitate (10 g) was loaded into the reactor vessel at 400 °C for 30 minutes. In order to study the effects of catalyst amount at constant H2 flow rate, the catalyst were varied i.e. 5, 10, and 15 g. To investigate the effects of H2 flow rate were varied from 20, 40, and 60 mL.min-1 at constant catalyst amount. The composition of the products was analyzed by GC and GC-MS. The results showed that methyl palmitate conversion increase with the increasing of catalyst amount. The highest methyl palmitate conversion (45.62 %) and yield of cetyl alcohol (36.44 %) were obtained for 15 g catalyst and 40 mL. min-1 H2 flow rate.
PENGARUH TRITERPEN TOTAL PEGAGAN (Centella asiatica (L) Urban) TERHADAP FUNGSI KOGNITIF BELAJAR DAN MENGINGAT PADA MENCIT JANTAN ALBINO (Mus musculus) YANG DIHAMBAT DENGAN SKOPOLAMIN
Authors: Herlina Herlina 
Published: February 2017

Pegagan (Centella asiatica (L) Urban) has been described to posses CNS effects such as improving cognitive function, learning and memory. The aim of the research was to evaluate the effects of total triterpen’s pegagan extract on cognitive functions as the learning and memory performance in male albino mice (Mus musculus) inhibited by scopolamine. The research design was Complete Randomized Design (RAL) – factorial on thirty six mice divided into 4 groups. One control group received only aquabidest (negative control). Three treatment groups received total triterpen 16 mg/kg BW, 32 mg/kg BW orally and piracetam 500 mg/kg BW by intra peritoneally (positive control) for 21 days. Data indicating learning and memory process of all subjects were obtained from one-trial passive avoidance test. Data were analyzed by two way ANOVA and BNT (p<0,05). Result showed that total triterpen 32 mg/kg BW had significantly prolonged the retention time compared to control group indicating improvement in cognitive function (505,03 second vs -18,53 second) (p<0,05) and it was not significantly different to piracetam 500 mg/kg BW group (505,03 second vs 522,48 second) (p>0,05). In conclusion, total triterpen from pegagan (Centella asiatica (L) Urban) improved learning ability and memory of male albino mice (Mus musculus) even though, it was inhibited by scopolamine.
PEMBUATAN DEKSTRIN DARI PATI UBI KAYU MENGGUNAKAN KATALIS AMILASE HASIL FRAKSINASI DARI Azospirillum sp. JG3PEMBUATAN DEKSTRIN DARI PATI UBI KAYU MENGGUNAKAN KATALIS AMILASE HASIL FRAKSINASI DARI Azospirillum sp. JG3
Authors: Zusfahair Zusfahair , Riana Ningsih Dian 
Published: February 2017

Amilase merupakan salah satu enzim potensial dalam proses industri. Amilase dapat diperoleh dari bakteriAzopsirillum sp. JG3. Amilase ini dapat dimanfaatkan untuk hidrolisis pati ubi kayu pada pembuatan dekstrin. Dekstrin memiliki peran yang cukup penting dalam industri seperti pembuatan roti, makanan bayi dan bahan penyalut lapis tipis tablet. Penelitian ini bertujuan untuk mengetahui karakterisasi biokimiawi amilase hasil fraksinasi dari Azospirillum sp. JG3 serta potensinya dalam pembuatan dekstrin.Tahap penelitian ini dimulai dengan memproduksi enzim yang dilakukan dengan cara menumbuhkan inokulum ke dalam medium produksi selama 30 jam kemudian diekstraksi dengan sentrifugasi sehingga diperoleh ekstrak kasar. Ekstrak kasar amilase kemudian difraksinasi secara bertahap (15%, 30%, 45% dan 60%) dengan menggunakan ammonium sulfat dan didialisis. Fraksi yang diperoleh diukur aktivitas, volume dan kadar proteinnya. Fraksi dengan aktivitas spesifik tertinggi dikarakterisasi dan digunakan untuk membuat dekstrin dari pati ubi kayu. Dekstrin pati ubi kayu yang diperoleh dikarakterisasi berupa rendemen, kadar air, kadar abu dandextrose equivalen.Hasil penelitian menunjukkan bahwa fraksi hasil dialisis 45% (FHD 45%) menghasilkan aktivitas dan aktivitas spesifik tertinggi berturut-turut sebesar 11,659 U/mL dan 7,319 U/mg. Aktivitas amilase optimum pada suhu 30°C, pH 7 dan konsentrasi substrat 4%. Dekstrin yang diperoleh memiliki rendemen 95,14, kadar air 3,411%, kadar abu 0,209% dan dextrose equivalen 13,4.
SCREENING DAN ANALISIS KADAR OMEGA-3 DARI RUMPUT LAUT PULAU LOMBOK NTB
Authors: Ryantin Gunawan Erin , Suhendra Dedy 
Published: February 2017

Penelitian ini mempunyai tujuan untuk mengidentifikasi jenis rumput laut yang mengandung omega-3 dan mengetahui kadar omega-3 dari rumput laut yang terdapat di pantai Pulau Lombok NTB. Minyak yang terdapat dalam rumput laut diekstraksi dengan metoda soxhletasi. Identifikasi dan kadar asam lemak dalam rumput laut ditentukan dengan alat GC-MS. Sembilan jenis rumput laut telah dianalisa dengan kadar air berkisar antara 42% -86,5%. Minyak hasil ekstraksi mempunyai kadar antara 0,63%-4,39%. Dari ke sembilan jenis dipilih 4 jenis rumput laut yang mempunyai kadar minyak yang paling tinggi. Dua jenis rumput laut yang biasa dikonsumsi (Eucheuma Spinosum dan Eucheuma Cottoni) dan dua jenis lagi yang tidak biasa dikonsumsi (Gracilaria salicorniadan Ulva sp). Asam-asam lemak omega-3 yang dapat teridentifikasi dari keempat jenis rumput laut adalah asam linolenat, Eikosatrienoat (ETE), eikosapentaenoat (EPA) dan (dokosaheksaenoat) DHA dalam jumlah yang bervariasi. Kadar asam lemak dalam keempat jenis rumput laut berkisar antara 26,8%-52,26% dan kandungan omega-3 antara 1,86%-5,46%.
SINTESIS DAN KARAKTERISASI GAMMA ALUMINA (γ-Al2O3) DARI KAOLIN ASAL TATAKAN, SELATAN BERDASARKAN VARIASI TEMPERATUR KALSINASI
Authors: Irawati Utami , Sunardi Sunardi , Suraida Suraida 
Published: February 2017

Sintesis dan karakterisasi gamma alumina (γ-Al2O3) dari kaolin asal Tatakan, Kalimantan Selatan berdasarkan variasi temperatur kalsinasi telah dilakukan. Gamma alumina (γ-Al2O3) diperoleh dengan metode sol-gel.Polyethylene glycol 6000 digunakan sebagai template pembentuk pori, presipitasi dilakukan dengan penambahan NH4OH 2,6 M hingga pH larutan 8-9 untuk memperoleh Al(OH)3. Kalsinasi dilakukan pada temperatur500, 600, 700 dan 800 0C. Gamma alumina (γ-Al2O3) hasil kalsinasi kemudian di analisis menggunakan Fourier Transform Infrared (FTIR) dan X-Ray Diftraction (XRD). Hasil analisis karakterisasi FTIR menunjukkan pada masing-masing variasi temperatur terbentuknya kerangka gamma alumina (γ-Al2O3) dan hasil XRD menunjukkan fasa alumina yang terbentuk adalah gamma alumina (γ-Al2O3). Struktur terbaik dari gamma alumina (γ-Al2O3) berdasarkan kekristalannya yaitu pada temperatur kalsinasi 700 0C dengan nilai 2θ = 46,06; 60,07 dan 66,93.
STUDI REAKSI KOPLING OKTADESILSILAN DENGAN ARIL IODIDA TERSUBSTITUSI PARA
Authors: Lesbani Aldes , Mohadi Risfidian , Hidayati Nurlisa , Elfita Elfita 
Published: February 2017

Telah dilakukan reaksi kopling oktadesilsilan dengan 4-iodida tioanisol dan 4-iodida anisol pada posisi para menggunakan katalis paladium dalam kondisi atmosfir argon/nitrogen menghasilkan senyawa tris-(4-tioanisil)-oktadesilsilan (1) dan tris-(4-metoksifenil)-oktadesilsilan (2)Hasil penelitian ini diperoleh senyawa (1) berupa kristal putih dengan titik leleh 59,8-60,5 oC dan puncak ion molekul sebesar m/z 650 Sedangkan untuk senyawa (2) mempunyai titik leleh 53,5-54,0 oC dengan nilai m/z 602. Karakterisasi menggunakan 1H dan 13C NMR menghasilkan puncak-puncak pergeseran kimia yang menunjukkan senyawa (1) adalah tris-(4-tioanisil)-oktadesilsilan dan senyawa (2) adalah tris-(4-metoksifenil)-oktadesilsilan (2)
DEODORISASI LIMBAH CAIR BATIK MENGGUNAKAN LIMBAH BAGLOG Pleurotus ostreatus DENGAN KOMBINASI VOLUME DAN WAKTU INKUBASI BERBEDA
Authors: Teguh Sumarko Heru , Lestari Sri , Stia Dewi Ratna 
Published: February 2017

Limbah cair batik yang dibuang ke lingkungan tanpa diolah terlebih dahulu bersifat toksik, mengakibatkan penurunan kualitas air disekitar lingkungan dan kesehatan dengan munculnya masalah utama seperti bau tidak sedap. Kondisi tersebut diperlukan penanganan agar efek pencemaran rendah atau menjadikan limbah cair batik tidak toksik. Penelitian tentang pengelolaan limbah cair batik berupa deodorisasi menggunakan limbah baglog Pleurotus ostreatus dengan kombinasi volume dan waktu inkubasi berbeda telah diujikan. Tujuan penelitian adalah untuk mengetahui kemampuan limbah baglog P. ostreatusdengan kombinasi volume dan waktu inkubasi berbeda dalam mendeodorisasi limbah cair batik, dan untuk mengetahui perlakuan yang terbaik dalam mendeodorisasi limbah cair batik menggunakan limbah baglog P. ostreatus dengan kombinasi volume dan waktu inkubasi berbeda. Hasil penelitian menunjukkan bahwa limbah baglog P. ostreatus dengan kombinasi volume dan waktu inkubasi berbeda mampu mendeodorisasi limbah cair batik. Hasil terbaik berupa penurunan skala bau 3 (tidak bau) yang diikuti persentase penurunan nilai BOD sebesar 93,95% (3301 mg/l menjadi 200 mg/l) dan COD 79,66% (15200 mg/l menjadi 3120 mg/l) pada perlakuan 100 ml volume limbah cair batik dan 96 jam waktu inkubasi.
KAJIAN ADSORPSI LINEAR ALKYL BENZENE SULPHONATE (LAS) DENGAN BENTONIT-KITOSAN
Authors: Rifai Miftah , Nugraha Irwan 
Published: February 2017

Deterjen pada umumnya tersusun atas surfaktan anionik seperti LAS (Linear alkyl Benzene sulphonate). pada percobaan ini LAS dapat menyerap sinar pada daerahuv dengan panjang gelombang maksimumnya adalah 223,5 nm. LAS dalam perairan dapat menimbulkan potensi masalah pencemaran.Tujuan dari penelitian ini adalah sebagai salah satu alternatif penanganan masalah pencemaran limbah domestik dengan cara menguji kinerja bentonit alam dan bentonit termodifikasi kitosan dalam mengadsorpsi LAS. Untuk mengetahui bahwa kitosan telah bereaksi dengan bentonit maka diuji dengan FTIR dan XRD pada bentonit alam dan bentonit-kitosan. Kemudian ditentukan waktu kontak optimum antara LAS dengan bentonit alam dan bentonit-kitosan. Serta penentuan isoterm adsorpsi LAS dengan bentonit alam dan bentonit-kitosan dengan cara membuat variasi konsentrasi larutan LAS. Uji kinerja bentonit alam dan bentonit-kitosan dalam mengadsorpsi LAS dilakukan dengan menggunakan sistem batch. Didapat waktu kontak optimum antara LAS berinteraksi dengan bentonit alam adalah 15 menit dengan kapasitas adsorpsi sebesar 3,265 mg/g. Sedangkan Waktu kontak optimum interaksi LAS dengan bentonit-kitosan terjadi pada waktu 15 menit dengan kapasitas adsorpsi sebesar 1,7mg/g. Dari hasil yang didapat maka dapat terlihat bahwa bentonit alam memiliki kapasitas adsorpsi yang lebih besar dibandingkan bentonit hasil modifikasi dengan kitosan. Interaksi antara bentonit alam dan bentonit–kitosan dengan LAS terjadi secara fisik dengan energi adsorpsi bentonit alam dengan LAS adalah 19,31 KJ/mol dan energi adsorpsi bentonit-kitosan dengan LAS adalah 19,60 KJ/mol.
KARAKTERISASI PAPAIN DARI DAUN PEPAYA (Carica Papaya L. CHARACTERIZATION OF PAPAIN FROM Carica Papaya L. LEAVES
Authors: Zusfahair Zusfahair , Riana Ningsih Dian , Nur Habibah Febrina 
Published: February 2017

Enzim yang menempati urutan pertama dalam pemanfaatannya di bidang industri adalah protease. Protease dapat digunakan sebagai katalis untuk reaksi yang menggunakan pelarut organik. Penelitian ini bertujuan untuk mengetahui karakteristik ekstrak kasar papain dari daun pepaya (Carica papaya L.) yang meliputi suhu dan pH optimum, pengaruh EDTA dan ion-ion logam, serta kestabilannya dalam pelarut organik seperti metanol, aseton, dan toluena, serta potensinya sebagai katalis dalam pelarut organik.Isolasi papain dari daun pepaya dilakukan untuk mendapatkan ekstrak kasar papain. Ekstrak kasar papain selanjutnya dikarakterisasi suhu dan pH optimum, pengaruh EDTA dan ion-ion logam yang meliputi ion Ca2+, ion Mg2+, Cu 2+, Zn2+, serta aktivitasnya dalam pelarut organik, seperti metanol, aseton, dan toluena.Hasil penelitian menunjukkan bahwa ekstrak kasar papain yang diisolasi dari daun pepaya kalifornia optimum pada suhu 60 oC dan pH 7, sedangkan papain daun pepaya bangkok optimum pada suhu 50 oC dan kisaran pH 7-8. Aktivitas enzim papain daun pepaya kalifornia dan bangkok meningkat dengan adanya ion Zn2+ dan menurun dengan adanya ion Ca2+, ion Mg2+, Cu2+ serta EDTA. Aktivitas papain daun pepaya kalifornia relatif stabil hingga jam ke-6 dengan penambahan pelarut metanol dan menurun setelah jam ke-3 dengan penambahan pelarut aseton dan toluena, sedangkan papain daun pepaya bangkok dengan penambahan pelarut metanol, aseton, ataupun toluena aktivitasnya hanya dapat stabil hingga jam ke-3. Papain dari daun pepaya kalifornia berpotensi digunakan sebagai biokatalis dalam pelarut metanol.