bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurologic late effects associated with radiologic evidence of vertebral osteoradionecrosis after salvage laryngectomy: A syndrome associated with survivors of laryngeal and hypopharyngeal cancer
Authors: Rosko Andrew J. , Spector Matthew E. , Griffin Garrett R. , Vainshtein Jeffrey M. , Lee Jae , Bradford Carol R. , Prince Mark E. P. , Moyer Jeffrey S. , Worden Francis P. , Eisbruch Avraham , Chepeha Douglas B. 
Published: April 2016

AbstractBackgroundDelayed nonspecific posterior neck pain after pharyngeal instrumentation can be associated with a syndrome of rapidly progressive neurologic embarrassment. We present this cohort to help define the syndrome and aid in early detection.MethodsWe conducted a retrospective case series of 6 patients presenting from 2003 to 2012 with a history of laryngeal or hypopharyngeal squamous cell carcinoma (SCC) who underwent radiotherapy (RT) or chemoradiotherapy (CRT) followed by salvage laryngectomy.ResultsPosterior neck and upper back pain developed a mean of 27.5 days after instrumentation of the pharynx (reconstruction after laryngectomy or pharyngeal dilation). Myelopathy developed an average of 21.5 days after the onset of posterior neck pain. Five patients required urgent decompression. Three patients developed quadriplegia. The disease‐specific mortality was 50%.ConclusionThere is a syndrome of late neurological effects after RT, salvage surgery, and pharyngeal instrumentation that is associated with high morbidity and mortality. © 2016 Wiley Periodicals, Inc. Head Neck 38:1187–1193, 2016
Oncologic outcomes of surgically treated early‐stage oropharyngeal squamous cell carcinoma
Authors: Kass Jason I. , Giraldez Laureano , Gooding William , Choby Garret , Kim Seungwon , Miles Brett , Teng Marita , Sikora Andrew G. , Johnson Jonas T. , Myers Eugene N. , Duvvuri Umamaheswar , Genden Eric M. , Ferris Robert L. 
Published: April 2016

AbstractBackgroundThe purpose of this study was to characterize oncologic outcomes in early (T1–T2, N0) and intermediate (T1–T2, N1) oropharyngeal squamous cell carcinoma (SCC) after surgery.MethodsPatients with oropharyngeal SCC treated with surgery were identified from 2 academic institutions.ResultsOf 188 patients, 143 met the inclusion criteria. Eighty‐six (60%) had T1 to T2 N0 and 57 (40%) had T1 to T2 N1 disease. Sixty‐five patients (45%) underwent a robotic‐assisted resection, whereas the remaining had transoral (n = 60; 42%), mandible‐splitting (n = 11; 8%), or transhyoid approaches (n = 7; 5%). Human papillomavirus (HPV) status was known for 97 patients (68%), and 54 (55%) were HPV positive. Three‐year recurrence‐free survival (RFS) was 82% (95% confidence interval [CI] = 0.75–0.89). Since 2008, HPV infection was protective of recurrence (log‐rank p = .0334). A single node did not increase the risk of recurrence (p = .467) or chance of a second primary (p = .175).ConclusionComplete surgical resection is effective therapy for early and intermediate oropharyngeal SCC. HPV‐negative patients were at increased risk for locoregional recurrence or second primary disease. © 2016 Wiley Periodicals, Inc. Head Neck 38: First–1471, 2016
Minimally invasive carcinosarcoma ex pleomorphic adenoma: A case report and literature review with cytohistological correlation
Authors: Mok Yingting , Min En Nga , Chwee Ming Lim , Petersson Fredrik 
Published: April 2016

AbstractBackgroundCarcinosarcoma of the salivary glands is a rare neoplasm, and the minimally invasive form constitutes a subgroup with a more favorable prognosis. The cytomorphologic features of this neoplasm can be appreciated on fine‐needle aspiration biopsy.Methods and ResultsWe present a patient with a minimally invasive carcinosarcoma ex non‐recurrent pleomorphic adenoma (Ca ex PA) who underwent initial fine‐needle aspiration biopsy followed by surgical resection. The tumor was composed predominantly of a light microscopic pleomorphic high‐grade sarcoma exhibiting partial myoepithelial immunohistochemical features, with a minor component of in situ and invasive salivary duct carcinoma (10%). A limited area with features of a hyalinized pleomorphic adenoma was identified.ConclusionThis is the third case report of the cytological features of Ca ex PA of the salivary gland, with histologic correlation. It further illustrates the oncogenic relationship between epithelial and myoepithelial elements in the early stages of carcinosarcomatous transformation. © 2016 Wiley Periodicals, Inc. Head Neck 38: E2483–E2489, 2016
Life cycle assessment comparison of photocatalytic coating and air purifier
Authors: Tichá Marie , Žilka Miroslav , Stieberová Barbora , Freiberg František 
Published: April 2016

Abstract
This article presents a comparison of 2 very different options for removal of undesirable microorganisms and airborne pollutants from the indoor environment of hospitals, schools, homes, and other enclosed spaces using air purifiers and photocatalytic coatings based on nano titanium dioxide (TiO2). Both products were assessed by life cycle assessment (LCA) methodology from cradle-to-grave. The assessment also includes comparison of 2 different nano TiO2 production technologies, one by continuous hydrothermal synthesis and the other by a sulfate process. Results of the study showed a relatively large contribution of photocatalytic coatings to reducing the effects of selected indices in comparison with an air purifier, regardless of which nano TiO2 production method is used. Although the impacts of the sulfate process are significantly lower compared to those of hydrothermal synthesis when viewed in terms of production alone, taken in the context of the entire product life cycle, the net difference becomes less significant. The study has been elaborated within the Sustainable Hydrothermal Manufacturing of Nanomaterials (SHYMAN) project, which aims to develop competitive and sustainable continuous nanoparticle (NP) production technology based on supercritical hydrothermal synthesis. Integr Environ Assess Manag 2016;12:478–485. © 2016 SETAC
Key Points
This comparative LCA study indicates that the use of FN2 photocatalytic coating to purify air in an enclosed space has a significantly lower environmental impact than the use of an air purifier for the same purpose. Illuminating a photocatalytic coating by natural light can significantly lower its environmental impact. A comparison of nanoparticle production methods indicates that the sulfate process creates the lowest environmental impact. Because of the low nanoparticle content in the product (photocatalytic coating) and the dominant influence of energy consumption in the use stage, the choice of nanoparticle production method has a minimal effect on the overall environmental impact of the product.
MicroRNA‐409‐3p regulates cell invasion and metastasis by targeting ZEB1 in breast cancer
Authors: Ma Zhenhai , Li Yang , Xu Jingchao , Ren Qiaozhen , Yao Jihong , Tian Xiaofeng 
Published: April 2016

AbstractMicroRNA‐409‐3p (miR‐409‐3p) is an miRNA expressed by embryonic stem cells, and our previous study demonstrated depressed miR‐409‐3p expression in human breast cancer (BC) cell lines; however, its role and function in BC metastasis are still unknown. The purpose of this study was to examine the expression levels of miR‐409‐3p in human BC and its role in the metastasis of BC. We analyzed the status of miR‐409‐3p expression in BC tissues by quantitative real‐time polymerase chain reaction (PCR) and its relationship to the clinicopathologic features of patients with BC. To study the role of miR‐409‐3p in BC metastasis, the invasion ability of BC cells was detected by transwell invasion assays and wound healing assays. WST‐1 assays and colony formation assays were used to investigate cell proliferation. Luciferase reporter assays were used to verify that miR‐409‐3p targeted zinc‐finger E‐box‐binding homeobox 1 (ZEB1). Western blot analyses and transwell assays were carried out to assess ZEB1 expression and its role in BC cell metastasis. The expression of miR‐409‐3p was lower in tumor tissues than in noncancerous breast tissues. We verified that miR‐409‐3p levels were downregulated and significantly correlated with poor outcomes in patients with BC. Overexpression of miR‐409‐3p inhibited cellular proliferation and suppressed cellular migration and invasion in vitro and in vivo. Dual‐luciferase reporter assays showed that miR‐409‐3p binds the 3′‐untranslated region (3′‐UTR) of ZEB1, suggesting that ZEB1 is a direct target of miR‐409‐3p. Western blot analysis confirmed that overexpression of miR‐409‐3p reduced ZEB1 protein levels. These data demonstrate that miR‐409‐3p plays an important role in regulating the metastasis of BC, which is involved in the post‐transcriptional repression of ZEB1. Our results indicate that miR‐409‐3p can regulate the invasion and metastasis process of BC by targeting ZEB1 and may serve as a new prognostic marker and therapeutic target for treating BC metastasis. © 2016 IUBMB Life, 68(5):394–402, 2016
Accounting for data variability, a key factor in in vivo/in vitro relationships: application to the skin sensitization potency (in vivo LLNA versus in vitro DPRA) example
Authors: Dimitrov S. , Detroyer A. , Piroird C. , Gomes C. , Eilstein J. , Pauloin T. , Kuseva C. , Ivanova H. , Popova I. , Karakolev Y. , Ringeissen S. , Mekenyan O. 
Published: April 2016

AbstractWhen searching for alternative methods to animal testing, confidently rescaling an in vitro result to the corresponding in vivo classification is still a challenging problem. Although one of the most important factors affecting good correlation is sample characteristics, they are very rarely integrated into correlation studies. Usually, in these studies, it is implicitly assumed that both compared values are error‐free numbers, which they are not. In this work, we propose a general methodology to analyze and integrate data variability and thus confidence estimation when rescaling from one test to another. The methodology is demonstrated through the case study of rescaling the in vitro Direct Peptide Reactivity Assay (DPRA) reactivity to the in vivo Local Lymph Node Assay (LLNA) skin sensitization potency classifications. In a first step, a comprehensive statistical analysis evaluating the reliability and variability of LLNA and DPRA as such was done. These results allowed us to link the concept of gray zones and confidence probability, which in turn represents a new perspective for a more precise knowledge of the classification of chemicals within their in vivo OR in vitro test. Next, the novelty and practical value of our methodology introducing variability into the threshold optimization between the in vitro AND in vivo test resides in the fact that it attributes a confidence probability to the predicted classification. The methodology, classification and screening approach presented in this study are not restricted to skin sensitization only. They could be helpful also for fate, toxicity and health hazard assessment where plenty of in vitro and in chemico assays and/or QSARs models are available. Copyright © 2016 John Wiley & Sons, Ltd.
Biomimetic hydrogel loaded with silk and l‐proline for tissue engineering and wound healing applications
Authors: Thangavel Ponrasu , Ramachandran Balaji , Kannan Ramya , Muthuvijayan Vignesh 
Published: April 2016

AbstractThe aim of this article was to develop silk protein (SF) and l‐proline (LP) loaded chitosan‐(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l‐proline, an important imino acid that is required for collagen synthesis, were added to chitosan to improve the wound healing properties of the hydrogel. Characterization of these hydrogels revealed that CS/SF/LP hydrogels were blended properly and LP incorporated hydrogels showed excellent thermal stability and good surface morphology. Swelling study showed the water holding efficiency of the hydrogels to provide enough moisture at the wound surface. In vitro biodegradation results demonstrated that the hydrogels had good degradation rate in PBS with lysozyme. LP loaded hydrogels showed approximately a twofold increase in antioxidant activity. In vitro cytocompatibility studies using NIH 3T3 L1 cells showed increased cell viability (p < 0.01), migration, proliferation and wound healing activity (p < 0.001) in LP loaded hydrogels compared to CS and CS/SF hydrogels. Cell adhesion on SF and LP hydrogels were observed using SEM and compared to CS hydrogel. LP incorporation showed 74‐78% of wound closure compared to 35% for CS/SF and 3% for CS hydrogels at 48 h. These results suggest that incorporation of LP can significantly accelerate wound healing process compared to pure CS and SF‐loaded CS hydrogels. Hence, CS/LP hydrogels could be a potential wound dressing material for the enhanced wound tissue regeneration and repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1401–1408, 2017.
Granulocytes and monocytes apheresis induces upregulation of TGFβ1 in patients with active ulcerative colitis: A possible involvement of soluble HLA‐I
Authors: Contini Paola , Negrini Simone , Bodini Giorgia , Trucchi Cecilia , Ubezio Gianluca , Strada Paolo , Savarino Vincenzo , Ghio Massimo 
Published: April 2016

Granulocyte and monocyte apheresis has been used in different immune‐mediated disorders, mainly inflammatory bowel diseases. The removal of activated leukocytes and several additional immunomodulatory mechanisms have been so far suggested to explain the anti‐inflammatory effects of the treatment. Recent data indicate that, during centrifugation based apheresis, sHLA‐I adsorbed to plastic circuits is able to induce TGFβ1 production in activated leukocytes. On these bases, the present study was aimed at analyzing if this model could be applied to a noncentrifugation based apheresis, such as granulocyte and monocyte apheresis. Ten patients with ulcerative colitis were enrolled. Every patient received 5 weekly apheresis treatments. Cellulose acetate beads removed from the column post‐GMA were stained by fluorescent anticlass I mAb and examined by fluorescent microscope. Moreover, sFasL plasma concentration, TGFβ1 plasma levels, and the percentage of TGFβ1 positive neutrophils were evaluated before and immediately after each single apheresis. Immunofluorescent images revealed a homogeneous layer of a sHLA‐I adsorbed to the surface of the beads recovered following the procedure. sFasL plasma concentration progressively increased both following the procedures and during inter‐procedure periods. Consistently, also TGFβ1 plasma levels and the percentage of TGFβ1 positive neutrophils increased during the procedures with a meaningful relationship with sFasL plasma levels. Taken together, these findings suggest that the immunosuppressive effects attributed to granulocyte and monocyte apheresis might depend, at least in part, on the sensitivity of activated leucocytes to the bioactivity of sHLA‐I molecules. J. Clin. Apheresis 32:49–55, 2017. © 2016 Wiley Periodicals, Inc.
Applying a new quantitative global breast MRI feature analysis scheme to assess tumor response to chemotherapy
Authors: Aghaei Faranak , Tan Maxine , Hollingsworth Alan B. , Zheng Bin 
Published: April 2016

PurposeTo develop a new quantitative global kinetic breast magnetic resonance imaging (MRI) features analysis scheme and assess its feasibility to assess tumor response to neoadjuvant chemotherapy.Materials and MethodsA dataset involving breast MR images acquired from 151 cancer patients before neoadjuvant chemotherapy was used. Among them, 63 patients had complete response (CR) and 88 had partial response (PR) to chemotherapy based on the RECIST criterion. A computer‐aided detection (CAD) scheme was applied to segment breast region depicted on the breast MR images and computed a total of 10 kinetic image features to represent parenchyma enhancement either from the entire two breasts or the bilateral asymmetry between the two breasts. To classify between CR and PR cases, we tested an attribution selected classifier that integrates with an artificial neural network and a Wrapper Subset Evaluator. The classifier was trained and tested using a leave‐one‐case‐out (LOCO)‐based cross‐validation method. The area under a receiver operating characteristic curve (AUC) was computed to assess classifier performance.ResultsFrom the pool of initial 10 features, four features were selected by more than 90% times in the LOCO cross‐validation iterations. Among them, three represent the bilateral asymmetry of kinetic features between two breasts. Using the classifier yielded AUC = 0.83 ± 0.04, which is significantly higher than using each individual feature to classify between CR and PR cases (P < 0.05).ConclusionThis study demonstrated that quantitative analysis of global kinetic features computed from breast MRI‐acquired prechemotherapy has potential to generate a useful clinical marker that is associated with tumor response to neoadjuvant chemotherapy. J. Magn. Reson. Imaging 2016;44:1099–1106.
Quantitative comparison of MR diffusion‐weighted imaging for liver focal lesions between 3.0T and 1.5T: Regions of interest of the minimum‐spot ADC, the largest possible solid part, and the maximum diameter in lesions
Authors: Tang Yanhua , Wang Haiyi , Wang Yingwei , Li Jie , Jia Rui , Ma Lu , Ye Huiyi 
Published: April 2016

PurposeTo quantitatively compare the apparent diffusion coefficient (ADC) values of 3.0T and 1.5T magnetic resonance (MR) diffusion‐weighted imaging (DWI) protocols using regions of interest (ROIs) for the minimum ADC, largest solid part, and maximum diameter of lesions, for the detection of liver focal lesions.Materials and MethodsIn total, 26 patients with 28 liver focal lesions prospectively underwent both 1.5T and 3.0T DWI of the liver. The protocols included respiratory‐triggered (RT), breath‐holding (BH), and free‐breathing (FB) acquisitions. The ADC values were measured at both field strengths using three methods: ROIs with the minimum ADC, the largest solid part, and the maximum diameter of lesions. Bland–Altman tests and paired t‐tests were used to compare ADC values in the liver focal lesions obtained at 1.5T and 3.0T.ResultsThe 3.0T and 1.5T protocols differed significantly with regard to the ADC values of the RT, BH, and FB acquisitions, for ROIs of both the largest solid part (P = 0.005, P = 0.014, and P = 0.022, respectively) and maximum diameter of lesions (P < 0.001, P = 0.001, and P = 0.001, respectively).ConclusionWhen using DWI for quantitative analysis of liver focal lesions, field strength could exert a negative effect depending on the ROI. The ADC values from ROIs of both the largest solid part and maximum diameter of lesions may differ between 1.5T and 3.0T protocols.J. Magn. Reson. Imaging 2016;44:1320–1329.
An intraoperative MRI system for margin assessment in breast conserving surgery: Initial results from a novel technique
Authors: Papa Moshe , Allweis Tanir , Karni Tami , Sandbank Judith , Konichezky Myriam , Diment Judith , Guterman Assaf , Shapiro Moshe , Peles Zachi , Maishar Roi , Gur Assaf , Kolka Eyal , Brem Rachel 
Published: April 2016

Background and ObjectivesOne of the major unmet needs in Breast Conserving Surgery (BCS) is a rapid and accurate margin assessment of the lumpectomy specimen. This study evaluates the ability of a novel MRI system (prototype of the ClearSight™ system; Clear‐Cut Medical Ltd., Rehovot, Israel) to distinguish malignant and non‐malignant tissues in freshly excised breast specimen by comparing MR measurements to histopathology results.MethodsSeventy‐seven samples were obtained from 22 patients undergoing BCS enrolled in the study. A T2* (T2 Star) value in milliseconds (ms) was calculated for each sample and correlated with histopathology results.ResultsOf the 77 samples, 35 samples were classified by histopathology as malignant and 42 as non‐malignant. T2* values were significantly higher in malignant samples compared to non‐malignant samples (15.3 ± 2.72 ms and 10.6 ± 1.47 ms, respectively [P < 0.00001]). Analysis for a determined cutoff of 11.7 ms revealed 91% sensitivity, 93% specificity, and 92% accuracy. ROC curve analysis yielded AUC of 0.97.ConclusionsThis study demonstrates that the system is sensitive and specific in differentiating malignant and non‐malignant tissues in freshly excised breast specimen. The system has the potential to be used for breast specimen margin assessment during BCS, with the goal of decreasing the need for re‐operation. J. Surg. Oncol. 2016;114:22–26. © 2016 Wiley Periodicals, Inc.
Social Causation Versus Social Erosion: Comparisons of Causal Models for Relations Between Support and PTSD Symptoms
Authors: Shallcross Sandra L. , Arbisi Paul A. , Polusny Melissa A. , Kramer Mark D. , Erbes Christopher R. 
Published: April 2016

AbstractSocial support is a robust correlate of posttraumatic stress disorder (PTSD) symptoms and of general psychological distress (Ozer, Best, Lipsey, & Weiss, 2003). The nature of the causal relationship between support and PTSD remains the subject of debate, with 2 models, social erosion and social causation, often used to explain findings. Despite extensive research using these models, no studies of which we are aware have included tests of both models within the same series of analyses, across more than 2 time points, in veterans. These competing models were tested in a sample of National Guard soldiers (N = 521) who completed measures of perceived social support and the PTSD Checklist‐Military version (Weathers, Litz, Herman, Huska, & Keane, 1993) at 3 months, 15 months, and 27 months following a combat deployment to Iraq. Analyses were run separately for overall PTSD symptoms and the PTSD components of intrusion, trauma‐avoidance, dysphoria, and hyperarousal. Both the social erosion (βs ranging from −.10 to −.19) and social causation (βs ranging from −.08 to −.13) hypotheses were supported. Results suggested PTSD‐specific symptom dimensions may both erode and be influenced by social support, whereas general psychological distress erodes social support. Implications for clinical intervention and research are discussed.
Amino‐Functional Polybenzimidazole Blends with Enhanced Phosphoric Acid Mediated Proton Conductivity as Fuel Cell Electrolytes
Authors: Aili David , Javakhishvili Irakli , Han Junyoung , Jankova Katja , Pan Chao , Hvilsted Søren , Jensen Jens Oluf , Bjerrum Niels J. , Li Qingfeng 
Published: April 2016

A new amino‐functional polybenzimidazole copolymer is synthesized by homogeneous solution condensation polymerization from a novel monomer, N,N′‐bis(2,4‐diaminophenyl)‐1,3‐diaminopropane. The copolymer readily dissolves in organic solvents and shows good film forming characteristics. To balance the phosphoric acid uptake and to obtain mechanically robust membranes, the amino‐functional polybenzimidazole derivative is blended with high molecular weight poly[2,2′‐(m‐phenylene)‐5,5′‐bisbenzimidazole] at different ratios. Due to the high acid uptake, the homogenous blend membranes show enhanced proton conductivity at temperatures well above 100 °C as also confirmed by the fuel cell polarization data.
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