bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forces of Federalism, Safety Nets, and Waivers
Authors: Stiglitz Edward H. 
Published: February 2017

AbstractInequality is the defining feature of our times. Many argue that it calls for a policy response, yet the most obvious policy responses require legislative action. And if inequality is the defining feature of our times, partisan acrimony and gridlock are the defining features of the legislature. That being so, it is worth considering what role administrative agencies, and administrative law, might play in ameliorating or exacerbating economic inequality. Here, I focus on American safety net programs, many of which are joint operations between federal administrative agencies and state governments. In this context, a central mode of bureaucratic policy innovation comes in the form of administrative waivers, whereby a federal administrative agency waives some statutory requirement that is otherwise binding on state administrators. For example, the Centers for Medicare and Medicaid Services recently granted waivers to allow several states to impose various “personal responsibility” requirements on Medicaid beneficiaries.Faced with a choice between legislative inactivity and policy innovation through waivers, many scholars and policymakers of both parties have tended to favor waivers. The appeal of waivers as a path around legislative gridlock is compelling. However, I argue that this view has neglected the federal structure of American safety net programs, and does not account for the state politics of implementation. Moreover, scholars have not focused on the severe information problems that federal agencies face when issuing waivers; a permissive waiver regime exacerbates these problems. Focusing on Medicaid implementation, I highlight the risks of waivers for American safety net programs. Before concluding, I discuss possible reforms to administrative procedures, and offer a case study of litigation surrounding one recent waiver application. The case study illustrates many of the theoretical arguments, and further demonstrates the failure of judicial review; it indicates how review might be adjusted to promote more effective use of waivers and diminish their perils.
The Effects of Enclosing Cultivated Land on the Physical Properties of Soil in the Loess Hill Region of Ordos
Authors: Wang Lin , Xue Fengna , Gao Wenbang , Shi Jing , Sun Shanshan , Liu Junxiu , Su Rina , Xie Yunhu , Hai Chunxing , Xiaojia Li 
Published: February 2017

Abstract
Protection of the environment by returning farmland to forest and grassland through enclosing areas of land to permit regeneration of native flora is being implemented in the Loess hill region of China. Soil physical properties are important components of ecological systems, as comparisons between cultivated and enclosed areas demonstrate. The results showed: the soil moisture content in the enclosed area was 14.6% and that in the cultivated area was 14%; the soil bulk density and soil porosity were respectively 1.45 g/cm3 and 45.28% in the enclosed areas, and respectively 1.46 g/cm3 and 44.79% in cultivated land. The alteration of soil physical properties was not big between cultivated areas and enclosed areas in the short term.
Riparian Vegetation on the Left Tributaries of the Danube Along the “Clisura” Cross Valley
Authors: Schneider-Binder Erika 
Published: February 2017

Abstract
The author presents the riparian vegetation of the left tributaries of the Danube in the “Clisura” area including the habitat types 91E0* Alluvial forests with Alnus glutinosa and Fraxinus excelsior (Alno-Padion, Alnion incanae, Salicion albae), 6430 Hydrophilous tall herb fringe communities and other interlocking habitats. They are analysed from the ecological and the phytocoenological point of view. Discussed are also their phytogeographical particularities. Furthermore are emphasized the changes of the vegetation on the lower part of the tributaries as a consequence of the construction of the Iron Gate power plant and the backwater situation. Finally the conservation status of the riparian habitats is analysed in the context of their importance for the European Natura 2000 network.
Reproductive Biology of Mangrove PlantsClerodendrum inerme, Derris trifoliata, Suaeda maritima, Suaeda monoica, Suaeda nudiflora
Authors: Aluri Jacob Solomon Raju , Kumar Rajendra , Chappidi Prasada Rao 
Published: February 2017

AbstractFloral biology, sexual system, breeding system, pollinators, fruiting and seed dispersal aspects of five mangrove associate species, namely,Clerodendrum inerme,Derris trifoliata,Suaeda maritima, S. monoica, andS. nudiflorawere studied. All these species are hermaphroditic, self-compatible, and exhibit mixed breeding systems adapted for pollination by external agents.C. inermeandD. trifoliataare principally entomophilous, whileSuaedaspecies are ambophilous. The study suggests that these plant species are important constituents of mangrove forests.C. inermeandD. trifoliataare useful in stabilizing the banks of back water creeks, whileSuaedaspecies are useful to carpet the saline areas and desalinate the soils which are subsequently useful for agricultural activities.
Conservation Status of Some Peatbogs in Maramureş County
Authors: Danci Oana 
Published: February 2017

AbstractPeat bogs and specially raised bogs are vulnerable ecosystems all over the world and they create refuges for some rare plant species. The aim of this paper is to provide information regarding the conservation status of five oligotrophic peat bogs situated in the volcanic mountains in the Oriental Chain of Carpathians, in Maramureș County. The studied peat bogs are: Tăul lui Dumitru (Dumitru Pond), Mlaștina Vlășinescu (Vlășinescu Peat bog), Iezeru Mare (Big Tarn), Tăul Chendroaiei (Chendroaiei Pond) and Tăul Negru (Black Pond), all of them have the status of nature reserve and under the IUCN categories they are included in category IV Habitat/Species Management Area (www.iucn.org, 2016). The analysis of the selected peat bogs reveals that all five of them have the vegetation included in the Natura 2000 priority habitat 7110* Active raised bogs. The conservation status of these peat bogs was evaluated and it is variable from moderate to very good conservation status.
The River Valleys as Biodiversity Reservoirs for Land Snails in Highly Anthropic Areas – The Case of Cisnădie River (Romania)
Authors: Gheoca Voichiţa 
Published: February 2017

Abstract
This study focuses on the snail fauna of a river valley passing through two closely located settlements. Thirty six species of terrestrial gastropods were identified. Species such as Macrogastra borealis, Alinda fallax, Alinda viridana, Bulgarica vetusta, Monachoides vicinus, Drobacia banatica, are present along the river and abundant in the sampling stations downstream of Cisnădie town. The high specific diversity and the presence of typical forest species demonstrate the presence of fragments of habitat that can preserve populations of terrestrial gastropods, underlining the importance of river valleys in conservation and dispersion of these species.
Alterations in Cytosolic and Mitochondrial [U-13C]Glucose Metabolism in a Chronic Epilepsy Mouse Model
Authors: McDonald Tanya S. , Carrasco-Pozo Catalina , Hodson Mark P. , Borges Karin 
Published: February 2017

AbstractTemporal lobe epilepsy is a common form of adult epilepsy and shows high resistance to treatment. Increasing evidence has suggested that metabolic dysfunction contributes to the development of seizures, with previous studies indicating impairments in brain glucose metabolism. Here we aim to elucidate which pathways involved in glucose metabolism are impaired, by tracing the hippocampal metabolism of injected [U-13C]glucose (i.p.) during the chronic stage of the pilocarpine-status epilepticus mouse model of epilepsy. The enrichment of13C in the intermediates of glycolysis and the TCA cycle were quantified in hippocampal extracts using liquid chromatography–tandem mass spectroscopy, along with the measurement of the activities of enzymes in each pathway. We show that there is reduced incorporation of13C in the intermediates of glycolysis, with the percentage enrichment of all downstream intermediates being highly correlated with those of glucose 6-phosphate. Furthermore, the activities of all enzymes in this pathway including hexokinase and phosphofructokinase were unaltered, suggesting that glucose uptake is reduced in this model without further impairments in glycolysis itself. The key findings were 33% and 55% losses in the activities of pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase, respectively, along with reduced13C enrichment in TCA cycle intermediates. This lower13C enrichment is best explained in part by the reduced enrichment in glycolytic intermediates, whereas the reduction of key TCA cycle enzyme activity indicates that TCA cycling is also impaired in the hippocampal formation. Together, these data suggest that multitarget approaches may be necessary to restore metabolism in the epileptic brain.
Distinct Effects of miR-210 Reduction on Neurogenesis: Increased Neuronal Survival of Inflammation But Reduced Proliferation Associated with Mitochondrial Enhancement
Authors: Voloboueva Ludmila A. , Sun Xiaoyun , Xu Lijun , Ouyang Yi-Bing , Giffard Rona G. 
Published: February 2017

Neurogenesis is essential to brain development and plays a central role in the response to brain injury. Stroke and head trauma stimulate proliferation of endogenous neural stem cells (NSCs); however, the survival of young neurons is sharply reduced by postinjury inflammation. Cellular mitochondria are critical to successful neurogenesis and are a major target of inflammatory injury. Mitochondrial protection was shown to improve survival of young neurons. This study tested whether reducing cellular microRNA-210 (miR-210) would enhance mitochondrial function and improve survival of young murine neurons under inflammatory conditions. Several studies have demonstrated the potential of miR-210 inhibition to enhance and protect mitochondrial function through upregulation of mitochondrial proteins. Here, miR-210 inhibition significantly increased neuronal survival and protected the activity of mitochondrial enzymes cytochromecoxidase and aconitase in differentiating NSC cultures exposed to inflammatory mediators. Unexpectedly, we found that reducing miR-210 significantly attenuated NSC proliferation upon induction of differentiation. Further investigation revealed that increased mitochondrial function suppressed the shift to primarily glycolytic metabolism and reduced mitochondrial length characteristic of dividing cells. Activation of AMP-regulated protein kinase–retinoblastoma signaling is important in NSC proliferation and the reduction of this activation observed by miR-210 inhibition is one mechanism contributing to the reduced proliferation. Postinjury neurogenesis occurs as a burst of proliferation that peaks in days, followed by migration and differentiation over weeks. Our studies suggest that mitochondrial protective miR-210 inhibition should be delayed until after the initial burst of proliferation, but could be beneficial during the prolonged differentiation stage.SIGNIFICANCE STATEMENTIncreasing the success of endogenous neurogenesis after brain injury holds therapeutic promise. Postinjury inflammation markedly reduces newborn neuron survival. This study found that enhancement of mitochondrial function by reducing microRNA-210 (miR-210) levels could improve survival of young neurons under inflammatory conditions. miR-210 inhibition protected the activity of mitochondrial enzymes cytochromecoxidase and aconitase. Conversely, we observed decreased precursor cell proliferation likely due to suppression of the AMP-regulated protein kinase–retinoblastoma axis with miR-210 inhibition. Therefore, mitochondrial protection is a double-edged sword: early inhibition reduces proliferation, but inhibition later significantly increases neuroblast survival. This explains in part the contradictory published reports of the effects of miR-210 on neurogenesis.
Hebbian Spike-Timing Dependent Plasticity at the Cerebellar Input Stage
Authors: Sgritta Martina , Locatelli Francesca , Soda Teresa , Prestori Francesca , D'Angelo Egidio Ugo 
Published: February 2017

Spike-timing-dependent plasticity (STDP) is a form of long-term synaptic plasticity exploiting the time relationship between postsynaptic action potentials (APs) and EPSPs. Surprisingly enough, very little was known about STDP in the cerebellum, although it is thought to play a critical role for learning appropriate timing of actions. We speculated that low-frequency oscillations observed in the granular layer may provide a reference for repetitive EPSP/AP phase coupling. Here we show that EPSP-spike pairing at 6 Hz can optimally induce STDP at the mossy fiber–granule cell synapse in rats. Spike timing-dependent long-term potentiation and depression (st-LTP and st-LTD) were confined to a ±25 ms time-window. Because EPSPs led APs in st-LTP while APs led EPSPs in st-LTD, STDP was Hebbian in nature. STDP occurred at 6–10 Hz but vanished >50 Hz or <1 Hz (where only LTP or LTD occurred). STDP disappeared with randomized EPSP/AP pairing or high intracellular Ca2+buffering, and its sign was inverted by GABA-A receptor activation. Both st-LTP and st-LTD required NMDA receptors, but st-LTP also required reinforcing signals mediated by mGluRs and intracellular calcium stores. Importantly, st-LTP and st-LTD were significantly larger than LTP and LTD obtained by modulating the frequency and duration of mossy fiber bursts, probably because STDP expression involved postsynaptic in addition to presynaptic mechanisms. These results thus show that a Hebbian form of STDP occurs at the cerebellum input stage, providing the substrate for phase-dependent binding of mossy fiber spikes to repetitive theta-frequency cycles of granule cell activity.SIGNIFICANCE STATEMENTLong-term synaptic plasticity is a fundamental property of the brain, causing persistent modifications of neuronal communication thought to provide the cellular basis of learning and memory. The cerebellum is critical for learning the appropriate timing of sensorimotor behaviors, but whether and how appropriate spike patterns could drive long-term synaptic plasticity remained unknown. Here, we show that this can actually occur through a form of spike-timing-dependent plasticity (STDP) at the cerebellar inputs stage. Pairing presynaptic and postsynaptic spikes at 6–10 Hz reliably induced STDP at the mossy fiber–granule cell synapse, with potentiation and depression symmetrically distributed within a ±25 ms time window. Thus, STDP can bind plasticity to the mossy fiber burst phase with high temporal precision.
Amygdalar Gating of Early Sensory Processing through Interactions with Locus Coeruleus
Authors: Fast Cynthia D. , McGann John P. 
Published: February 2017

Fear- and stress-induced activity in the amygdala has been hypothesized to influence sensory brain regions through the influence of the amygdala on neuromodulatory centers. To directly examine this relationship, we used optical imaging to observe odor-evoked activity in populations of olfactory bulb inhibitory interneurons and of synaptic terminals of olfactory sensory neurons (the primary sensory neurons of the olfactory system, which provide the initial olfactory input to the brain) during pharmacological inactivation of amygdala and locus coeruleus (LC) in mice. Although the amygdala does not directly project to the olfactory bulb, joint pharmacological inactivation of the central, basolateral, and lateral nuclei of the amygdala nonetheless strongly suppressed odor-evoked activity in GABAergic inhibitory interneuron populations in the OB. This suppression was prevented by inactivation of LC or pretreatment of the olfactory bulb with a broad-spectrum noradrenergic receptor antagonist. Visualization of synaptic output from olfactory sensory neuron terminals into the olfactory bulb of the brain revealed that amygdalar inactivation preferentially strengthened the odor-evoked synaptic output of weakly activated populations of sensory afferents from the nose, thus demonstrating a change in sensory gating potentially mediated by local inhibition of olfactory sensory neuron terminals. We conclude that amygdalar activity influences olfactory processing as early as the primary sensory input to the brain by modulating norepinephrine release from the locus coeruleus into the olfactory bulb. These findings show that the amygdala and LC state actively determines which sensory signals are selected for processing in sensory brain regions. Similar local circuitry operates in the olfactory, visual, and auditory systems, suggesting a potentially shared mechanism across modalities.SIGNIFICANCE STATEMENTThe affective state is increasingly understood to influence early neural processing of sensory stimuli, not just the behavioral response to those stimuli. The present study elucidates one circuit by which the amygdala, a critical structure for emotional learning, valence coding, and stress, can shape sensory input to the brain and early sensory processing through its connections to the locus coeruleus. One function of this interaction appears to be sensory gating, because inactivating the central, basolateral, and lateral nuclei of the amygdala selectively strengthened the weakest olfactory inputs to the brain. This linkage of amygdalar and LC output to primary sensory signaling may have implications for affective disorders that include sensory dysfunctions like hypervigilance, attentional bias, and impaired sensory gating.
Cannabinoids Activate Monoaminergic Signaling to Modulate KeyC. elegansBehaviors
Authors: Oakes Mitchell D. , Law Wen Jing , Clark Tobias , Bamber Bruce A. , Komuniecki Richard 
Published: February 2017

Cannabis sativa, or marijuana, a popular recreational drug, alters sensory perception and exerts a range of potential medicinal benefits. The present study demonstrates that the endogenous cannabinoid receptor agonists 2-arachidonoylglycerol (2-AG) and anandamide (AEA) activate a canonical cannabinoid receptor inCaenorhabditis elegansand also modulate monoaminergic signaling at multiple levels. 2-AG or AEA inhibit nociception and feeding through a pathway requiring the cannabinoid-like receptor NPR-19. 2-AG or AEA activate NPR-19 directly and cannabinoid-dependent inhibition can be rescued innpr-19-null animals by the expression of a human cannabinoid receptor, CB1, highlighting the orthology of the receptors. Cannabinoids also modulate nociception and locomotion through an NPR-19-independent pathway requiring an α2A-adrenergic-like octopamine (OA) receptor, OCTR-1, and a 5-HT1A-like serotonin (5-HT) receptor, SER-4, that involves a complex interaction among cannabinoid, octopaminergic, and serotonergic signaling. 2-AG activates OCTR-1 directly. In contrast, 2-AG does not activate SER-4 directly, but appears to enhance SER-4-dependent serotonergic signaling by increasing endogenous 5-HT. This study defines a conserved cannabinoid signaling system inC. elegans, demonstrates the cannabinoid-dependent activation of monoaminergic signaling, and highlights the advantages of studying cannabinoid signaling in a genetically tractable whole-animal model.SIGNIFICANCE STATEMENTCannabis sativa, or marijuana, causes euphoria and exerts a wide range of medicinal benefits. For years, cannabinoids have been studied at the cellular level using tissue explants with conflicting results. To better understand cannabinoid signaling, we have used theCaenorhabditis elegansmodel to examine the effects of cannabinoids on behavior. The present study demonstrates that mammalian cannabinoid receptor ligands activate a conserved cannabinoid signaling system inC. elegansand also modulate monoaminergic signaling, potentially affecting an array of disorders, including anxiety and depression. This study highlights the potential role of cannabinoids in modulating monoaminergic signaling and the advantages of studying cannabinoid signaling in a genetically tractable, whole-animal model.
Structural studies of RFCCtf18 reveal a novel chromatin recruitment role for Dcc1
Authors: Wade Benjamin O , Liu Hon Wing , Samora Catarina P , Uhlmann Frank , Singleton Martin R 
Published: February 2017

AbstractReplication factor C complexes load and unload processivity clamps from DNA and are involved in multiple DNA replication and repair pathways. The RFCCtf18 variant complex is required for activation of the intra‐S‐phase checkpoint at stalled replication forks and aids the establishment of sister chromatid cohesion. Unlike other RFC complexes, RFCCtf18 contains two non‐Rfc subunits, Dcc1 and Ctf8. Here, we present the crystal structure of the Dcc1‐Ctf8 heterodimer bound to the C‐terminus of Ctf18. We find that the C‐terminus of Dcc1 contains three‐winged helix domains, which bind to both ssDNA and dsDNA. We further show that these domains are required for full recruitment of the complex to chromatin, and correct activation of the replication checkpoint. These findings provide the first structural data on a eukaryotic seven‐subunit clamp loader and define a new biochemical activity for Dcc1.
A Population Genomics Approach to Assessing the Genetic Basis of Within-Host Microevolution Underlying Recurrent Cryptococcal Meningitis Infection
Authors: Rhodes Johanna , Beale Mathew A , Vanhove Mathieu , Jarvis Joseph N , Kannambath Shichina , Simpson John A , Ryan Anthea , Meintjes Graeme , Harrison Thomas S , Fisher Matthew C , Bicanic Tihana 
Published: February 2017

AbstractRecurrence of meningitis due to Cryptococcus neoformans after treatment causes substantial mortality in HIV/AIDS patients across sub-Saharan Africa. In order to determine whether recurrence occurred due to relapse of the original infecting isolate or reinfection with a different isolate weeks or months after initial treatment, we used whole-genome sequencing (WGS) to assess the genetic basis of infection in 17 HIV-infected individuals with recurrent cryptococcal meningitis (CM). Comparisons revealed a clonal relationship for 15 pairs of isolates recovered before and after recurrence showing relapse of the original infection. The two remaining pairs showed high levels of genetic heterogeneity; in one pair we found this to be a result of infection by mixed genotypes, while the second was a result of nonsense mutations in the gene encoding the DNA mismatch repair proteins MSH2, MSH5, and RAD5. These nonsense mutations led to a hypermutator state, leading to dramatically elevated rates of synonymous and nonsynonymous substitutions. Hypermutator phenotypes owing to nonsense mutations in these genes have not previously been reported in C. neoformans, and represent a novel pathway for rapid within-host adaptation and evolution of resistance to first-line antifungal drugs.