bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRAF6 Mediates Suppression of Osteoclastogenesis and Prevention of Ovariectomy-Induced Bone Loss by a Novel Prenylflavonoid
Authors: Tan Ee Min , Li Lei , Indran Inthrani Raja , Chew Nicholas , Yong Eu-Leong 
Published: November 2016

ABSTRACT

Given the limitations of current therapeutic options for postmenopausal osteoporosis, there is a need for alternatives with minimal adverse effects. In this study, we evaluated the effects of icaritin (ICT), a natural prenylflavonoid, on osteoclastogenesis both in vitro and in an ovariectomized (OVX) rat model and investigated its underlying molecular mechanism(s) of action. ICT inhibited osteoclast formation in two osteoclast precursor models, RAW 264.7 mouse monocyte cell line and human PBMC. ICT also inhibited sealing zone and resorption pit formation in a dose-dependent manner. Mechanistically, ICT inhibited RANKL-induced NF-κB and MAPK/AP-1 pathways to suppress gene expression of nuclear factor of activated T cells (NFAT)c1, the master transcription regulator of osteoclast differentiation. ICT, by inhibiting the TRAF6/c-Src/PI3K pathway, suppressed NADPH oxidase-1 activation to attenuate intracellular ROS production and downregulate calcineurin phosphatase activity. As a result, NFATc1 nuclear translocation and activity was suppressed. Crucially, ICT promoted proteasomal degradation of TRAF6, the critical adaptor protein that transduces RANKL/RANK signaling, and the inhibitory effect of ICT on osteoclastogenesis was reversed by the proteasomal inhibitor MG 132. ICT administration inhibited OVX-induced bone loss and resorption by suppressing osteoclast formation and activity. Consistent with cellular studies, ICT downregulated TRAF6 and NFATc1 protein expression in CD11b+/Gr-1-/low osteoclast precursors isolated from OVX rats. Put together, we present novel findings that ICT, by downregulating TRAF6, coordinates inhibition of NF-κB, MAPK/AP-1, and ROS signaling pathways to reduce expression and activity of NFATc1. These results demonstrate the potential of ICT for treatment of postmenopausal osteoporosis and point to TRAF6 as a promising target for novel anti-osteoporotic drugs. © 2017 American Society for Bone and Mineral Research.
Four cases of pulmonary thromboembolism diagnosed by endobronchial ultrasound
Authors: Erer Onur Fevzi , Cimen Pinar , Unlu Mehmet , Katgi Nuran 
Published: November 2016

ABSTRACTEndobronchial ultrasound (EBUS) is a technique for the detection of mediastinal abnormalities. A total of 548 EBUS procedures were performed for various indications, and in four cases (0.7%), filling defects in central pulmonary arteries were demonstrated fortuitously during the procedure. Subsequently, all patients underwent contrast‐enhanced CT of the thorax to confirm the diagnosis of pulmonary thromboembolism (PTE). In three of the four cases, there was a concomitant lung cancer. PTE can be incidentally detected during EBUS; therefore, pulmonary arteries should be examined carefully during EBUS in all patients, particularly in patients with suspected or proven malignancy. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 45:441–444, 2017
Preparation and preclinical evaluation of 131I‐trastuzumab for breast cancer
Authors: Kameswaran Mythili , Gota Vikram , Ambade Rajwardhan , Gupta Sudeep , Dash Ashutosh 
Published: November 2016

Trastuzumab that targets the human epidermal growth factor receptor type 2 (HER2) is known to benefit patients with HER2+ metastatic breast cancer. The objective was to explore the potential of 131I‐trastuzumab for treatment of breast cancers. Radioiodination of trastuzumab was carried out by chloramine‐T method, purified by using PD‐10 column, and characterized by size exclusion high‐performance liquid chromatography on a gel column. In vitro studies were carried out in HER2+ cells to determine the specificity of the radioimmunoconjugate. Uptake and retention of 131I‐trastuzumab were determined by biodistribution studies in tumor‐bearing non‐obese diabetic/severe combined immunodeficiency and normal severe combined immunodeficiency mice. The radiochemical purity (RCP) of 131I‐trastuzumab was 98 ± 0.4% with retention time of 17 minutes by high‐performance liquid chromatography. In vitro stability studies exhibited RCP of more than 90% in serum at 37°C after 120 hours of radioiodination. In vitro cell binding with 131I‐trastuzumab in HER2+ cells showed binding of 28% to 35% which was inhibited significantly, with unlabeled trastuzumab confirming its specificity. Kd value of 131I‐trastuzumab was 0.5 nM, while its immunoreactivity was more than 80%. Uptake of more than 12% and retention were observed in the tumors up to 120 hours p.i. 131I‐trastuzumab prepared in‐house‐exhibited RCP of more than 98%, excellent immunoreactivity, affinity to HER2+ cell lines and good tumor uptake thereby indicating its potential for further evaluation in HER2+ breast cancers.
Polymerization of supramonomers: A new way for fabricating supramolecular polymers and materials
Authors: Song Qiao , Xu Jiang‐Fei , Zhang Xi 
Published: November 2016

ABSTRACTSupramolecular polymers and materials are attracting more and more attention nowadays due to their dynamic properties such as reversibility, stimuli‐responsiveness and self‐healing. Conventionally, bifunctional or multi‐functional monomers are first covalently synthesized, followed by the supramolecular complexation to form supramolecular polymers and materials. Recently, we have proposed the supramonomer concept to construct supramolecular polymers and materials in a different way. Supramonomers are bifunctional or multi‐functional monomers fabricated by noncovalent synthesis, but can undergo traditional covalent polymerization. In this highlight article, we will summarize and discuss the fabrication of supramonomer and covalent polymerization methods of supramonomers; fabrication of multi‐responsive supramolecular polymers from supramonomers; and fabrication of supramolecular materials from supramonomers. It is highly anticipated that the supramonomer concept will enrich the methodology towards supramolecular polymers and materials. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 604–609
Complex coacervate‐based materials for biomedicine
Authors: Blocher Whitney C. , Perry Sarah L. 
Published: November 2016

There has been increasing interest in complex coacervates for deriving and transporting biomaterials. Complex coacervates are a dense, polyelectrolyte‐rich liquid that results from the electrostatic complexation of oppositely charged macroions. Coacervates have long been used as a strategy for encapsulation, particularly in food and personal care products. More recent efforts have focused on the utility of this class of materials for the encapsulation of small molecules, proteins, RNA, DNA, and other biomaterials for applications ranging from sensing to biomedicine. Furthermore, coacervate‐related materials have found utility in other areas of biomedicine, including cartilage mimics, tissue culture scaffolds, and adhesives for wet, biological environments. Here, we discuss the self‐assembly of complex coacervate‐based materials, current challenges in the intelligent design of these materials, and their utility applications in the broad field of biomedicine. WIREs Nanomed Nanobiotechnol 2017, 9:e1442. doi: 10.1002/wnan.1442This article is categorized under:

Therapeutic Approaches and Drug Discovery > Emerging Technologies
Diagnostic Tools > In Vitro Nanoparticle-Based Sensing
Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement
Theories for Psychotherapeutic Genetic Counseling: Fuzzy Trace Theory and Cognitive Behavior Theory
Authors: Biesecker Barbara , Austin Jehannine , Caleshu Colleen 
Published: November 2016

AbstractPsychotherapeutic genetic counseling is an increasingly relevant practice description. In this paper we aim to demonstrate how psychotherapeutic genetic counseling can be achieved by using psychological theories to guide one's approach to working with clients. We describe two illustrative examples, fuzzy trace theory and cognitive behavior theory, and apply them to two challenging cases. The theories were partially derived from evidence of beneficial client outcomes using a psychotherapeutic approach to patient care in other settings. We aim to demonstrate how these two specific theories can inform psychotherapeutic genetic counseling practice, and use them as examples of how to take a psychological theory and effectively apply it to genetic counseling.
Is Divorce More Painful When Couples Have Children? Evidence From Long-Term Panel Data on Multiple Domains of Well-being
Authors: Leopold Thomas , Kalmijn Matthijs 
Published: November 2016

AbstractTheoretical models of the divorce process suggest that marital breakup is more painful in the presence of children, yet little is known about the role of children as a moderator of divorce effects on adult well-being. The present study addresses this gap of research based on long-term panel data from Germany (SOEP). Following individuals over several years before and after divorce, we investigated whether the impact of divorce on multiple measures of well-being varied by the presence and age of children before marital breakup. Three central findings emerged from the analysis. First, declines in well-being were sharper in the presence of children, and these moderator effects were larger if children were younger. Second, domain-specific measures of well-being revealed gender differences in the moderating role of children. Mothers sustained deeper drops in economic well-being than did fathers; the reverse was true for family well-being. Third, most of these disproportionate declines in the well-being of divorced parents did not persist in the long term given that higher rates of adaptation leveled out the gaps compared with childless divorcees.
Forward psychiatry – early intervention for mental health problems among UK armed forces in Afghanistan
Authors: Jones N. , Fear N.T. , Wessely S. , Thandi G. , Greenberg N. 
Published: November 2016

AbstractBackgroundThis observational study examined return to duty (RTD) rates following receipt of early mental health interventions delivered by deployed mental health practitioners.MethodIn-depth clinical interviews were conducted among 975 UK military personnel referred for mental health assessment whilst deployed in Afghanistan. Socio-demographic, military, operational, clinical and therapy outcomes were recorded in an electronic health record database. Rates and predictors of EVAC were the main outcomes examined using adjusted binary logistic regression analyses.ResultsOverall 74.8% (n= 729) of personnel RTD on completion of care. Of those that underwent evacuation home (n= 246), 69.1% (n= 170) returned by aeromedical evacuation; the remainder returned home using routine air transport. Predictors of evacuation included; inability to adjust to the operational environment, family psychiatric history, previously experiencing trauma and thinking about or carrying out acts of deliberate self-harm.ConclusionDeployed mental health practitioners helped to facilitate RTD for three quarters of mental health casualties who consulted with them during deployment; psychological rather than combat-related factors predicted evacuation home.
Nociceptor‐like rat dorsal root ganglion neurons express the angiotensin‐II AT2 receptor throughout development
Authors: Benitez Sergio , Seltzer Alicia , Acosta Cristian 
Published: November 2016

AbstractAT2 receptor (AT2R) plays a functional role in foetal development. Its expression declines in most tissues soon after birth but stays high in sensory areas of the adult nervous system. In the dorsal root ganglia (DRG) the expression pattern of AT2R during development and the identity of the subpopulation expressing it remain unknown. Using a combination of semi‐quantitative PCR, western blotting and immunohistochemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonic day 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibited only minor (statistically non‐significant) fluctuations from E15 to PN30. Detailed quantitative analysis of ABC/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 and b) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons are putative C‐nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whether these AT2R‐positive neurons co‐localized with Ret and trkA embryonically and with IB4‐binding postnatally. Most AT2R‐positive neurons expressed trkA embryonically and bound IB4 postnatally. We found strong positive statistically highly significant correlations between AT2R cytoplasmic%intensities and trkA at E15/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated with IB4‐binding at PN3, 15 and 30. Our demonstration that a subpopulation of C‐nociceptor‐like neurons expresses AT2R during development supports a role for this receptor in neuropathic pain.
Functional Magnetic Resonance Imaging for Preoperative Planning in Brain Tumour Surgery
Authors: Lau Jonathan C. , Kosteniuk Suzanne E. , Bihari Frank , Megyesi Joseph F. 
Published: November 2016

AbstractBackground:Functional magnetic resonance imaging (fMRI) is being increasingly used for the preoperative evaluation of patients with brain tumours.Methods:The study is a retrospective chart review investigating the use of clinical fMRI from 2002 through 2013 in the preoperative evaluation of brain tumour patients. Baseline demographic and clinical data were collected. The specific fMRI protocols used for each patient were recorded.Results:Sixty patients were identified over the 12-year period. The tumour types most commonly investigated were high-grade glioma (World Health Organization grade III or IV), low-grade glioma (World Health Organization grade II), and meningioma. Most common presenting symptoms were seizures (69.6%), language deficits (23.2%), and headache (19.6%). There was a predominance of left hemispheric lesions investigated with fMRI (76.8% vs 23.2% for right). The most commonly involved lobes were frontal (64.3%), temporal (33.9%), parietal (21.4%), and insular (7.1%). The most common fMRI paradigms were language (83.9%), motor (75.0%), sensory (16.1%), and memory (10.7%). The majority of patients ultimately underwent a craniotomy (75.0%), whereas smaller groups underwent stereotactic biopsy (8.9%) and nonsurgical management (16.1%). Time from request for fMRI to actual fMRI acquisition was 3.1±2.3 weeks. Time from fMRI acquisition to intervention was 4.9±5.5 weeks.Conclusions:We have characterized patient demographics in a retrospective single-surgeon cohort undergoing preoperative clinical fMRI at a Canadian centre. Our experience suggests an acceptable wait time from scan request to scan completion/analysis and from scan to intervention.
National Perspectives on the Training of Neurosurgery Residents in Stereotactic Radiosurgery
Authors: Mansouri Alireza , Witiw Christopher D. , Badhiwala Jetan H. , Nassiri Farshad , McDonald Patrick J. , Kulkarni Abhaya V. , Zadeh Gelareh , Kondziolka Douglas 
Published: November 2016

AbstractBackground:Despite the critical role played by neurosurgeons in performing radiosurgery, neurosurgery residents in Canada have limited exposure to radiosurgery during their training. A survey of neurosurgery residents and faculty along with radiation oncology faculty was conducted to analyze perspectives regarding incorporating formal radiosurgery training into the neurosurgery residency curriculumMethods:An online survey platform was employed. Descriptive statistics were used to summarize center and respondent characteristics. Categorical variables were compared using odds ratios and corresponding 95% confidence intervals. The chi-squared test was utilized to assess statistical significance. A value ofp<0.05 was considered significantResults:The response rate was 31% (119/381); 87% (102/119) of respondents were from the neurosurgical specialty and 13% (17/119) from radiation oncology. Some 46% of residents (18/40) were “very uncomfortable” with radiosurgery techniques, and 57% of faculty (42/73) believed that dedicated radiosurgery training would be beneficial though impractical. No respondents felt that “no training” would be beneficial. A total of 46% of residents (19/41) felt that this training would be beneficial and that time should be taken away from other rotations, if needed, while 58% of faculty (42/73) and 75% (28/41) of residents believed that either 1 or 1-3 months of time dedicated to training in radiosurgery would sufficeConclusions:Canadian neurosurgeons are actively involved in radiosurgery. Despite residents anticipating a greater role for radiosurgery in their future, they are uncomfortable with the practice. With the indications for radiosurgery expanding, this training gap can have serious adverse consequences for patients. Considerations regarding the incorporation and optimal duration of dedicated radiosurgery training into the Canadian neurosurgery residency curriculum are necessary.
Collagen Extraction and Stable Isotope Analysis of Small Vertebrate Bones: A Comparative Approach
Authors: Cersoy S , Zazzo A , Lebon M , Rofes J , Zirah S 
Published: November 2016

AbstractBone remains of small vertebrate fossils provide valuable information for paleoenvironmental and paleoclimatic reconstructions. However, direct radiocarbon dating of small vertebrates remains challenging as the extraction of sufficient good quality collagen is required. The efficiency of eight collagen extraction protocols was tested on seven samples, representative of different ages and burial environments, including both macro and small vertebrate taxa. First, the samples were prescreened using attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) to quantify collagen content in archaeological bones, revealing that one should be discarded for 14C dating. Then, the quantity of protein extracted (yield) and collagen integrity were checked using conventional elemental analysis. The results show that one protocol was not able to accurately extract collagen from the samples. A soft HCl-based protocol seems more appropriate for the pretreatment of archaeological small mammal bones, whereas a harsher protocol might be more efficient to extract a higher amount of collagen from large mammals as well as amphibian bones. The influence of the tested protocols on carbon and nitrogen isotope values was also investigated. The results showed that isotopic variability, when existing, is related to the interindividual differences rather than the different protocols.
Structural and kinematic analyses of the basement window within the hinterland fold-and-thrust belt of the Zagros orogen, Iran
Authors: SARKARINEJAD KHALIL , DERIKVAND SOMAYE 
Published: November 2016

AbstractThe Zagros hinterland fold-and-thrust belt is located in the central portion of the Zagros Thrust System and consists of the exhumed basement windows associated with NW-striking and NE-dipping flexural duplex structures that contain in-sequence thrusting and related folds. Mylonitic nappes of the basement were exhumed along deep-seated sole thrusts of the Zagros Thrust System. Lattice preferred orientation (LPO) c-axes of quartz show asymmetric type-1 crossed girdles that demonstrate a non-coaxial deformation under plane strain conditions. Based on the opening angles of quartz c-axis fabric skeletons, deformation temperatures vary from 425±50°C to 540±50°C, indicating amphibolite facies conditions. The estimated mean kinematic vorticity evaluated from quartz c-axis of the quartzo-feldspathic mylonites (Wm = 0.55±0.06) indicates the degree of non-coaxiality during mylonite exhumation. The estimated angle θ between the maximum instantaneous strain axis (ISA1) and the transpressional zone boundary is 17°, and the angle of oblique convergence is 57° in the M2 nappe of the basement involved. This indicates that the mylonitic nappe was formed by a combination of 62% pure shear and 38% simple shear during oblique convergence.
Eye movement patterns and visual attention during scene viewing in 3- to 12-month-olds
Authors: HELO ANDREA , RÄMÄ PIA , PANNASCH SEBASTIAN , MEARY DAVID 
Published: November 2016

AbstractRecently, two attentional modes have been associated with specific eye movement patterns during scene processing. Ambient mode, characterized by short fixations and long saccades during early scene inspection, is associated with localization of objects. Focal mode, characterized by longer fixations, is associated with more detailed object feature processing during later inspection phase. The aim of the present study was to investigate the development of these attentional modes. More specifically, we examined whether indications of ambient and focal attention modes are similar in infants and adults. Therefore, we measured eye movements in 3- to 12-months-old infants while exploring visual scenes. Our results show that both adults and 12-month-olds had shorter fixation durations within the first 1.5 s of scene viewing compared with later time phases (>2.5 s); indicating that there was a transition from ambient to focal processing during image inspection. In younger infants, fixation durations between two viewing phases did not differ. Our results suggest that at the end of the first year of life, infants have developed an adult-like scene viewing behavior. The evidence for the existence of distinct attentional processing mechanisms during early infancy furthermore underlines the importance of the concept of the two modes.
Monte Carlo study on effective source to surface distance for electron beams from a mobile dedicated IORT accelerator
Authors: Hosseini Aghdam Mir Rashid , Baghani Hamid Reza , Mahdavi Seyed Rabi , Aghamiri Seyed Mahmoud Reza , Akbari Mohammad Esmail 
Published: November 2016

AbstractPurposeThe effective source to surface distance (SSDeff) for different combinations of energy/applicator size of the electron beam produced by the light intraoperative accelerator, a mobile dedicated intraoperative radiotherapy accelerator, has been calculated in this study.MethodsBoth ionometric dosimetry and Monte Carlo (MC) simulation were followed to obtain the SSDefffor different combinations of electron energy/applicator size. Simulations were performed using Monte Carlo Nuclear Particles (MCNP) MC code. Measurements were performed by Advance Markus chamber and inside a polymethyl methacrylate slab phantom. Inverse square law method was employed to determine the SSDefffrom acquired dosimetry data.ResultWith increasing the applicator diameter at a given energy, SSDeffis also increased. The same result is obtained with increasing the electron beam energy for a given applicator size. The results of MC-based SSDefffor 10 cm diameter reference applicator at different energies were in a good accordance with those obtained by ionometric dosimetry. The maximum and mean differences between the results were 1·1 and 0·6%, respectively.ConclusionsThe results of this study showed that SSDeffof intraoperative electron beam is highly dependent on the applicator size and is a mild function of electron beam energy. These facts are in accordance with those reported for conventional electron beam. The good agreement between the results of MC simulation and ionometric dosimetry confirms the application of MCNP code in modelling of intraoperative electron beam and obtaining the intended parameters.