bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of fibronectin and laminin expression by Rhodium (II) citrate-coated maghemite nanoparticles in mice bearing breast tumor
Authors: Rocha Márcia , Arcanjo Rachel , Lopes Cláudio , Carneiro Marcella , Souza Aparecido , Báo Sônia 
Published: December 2017

AbstractDegradation of cellular matrix is one of the important processes related to the progression of breast cancer. Tumor cells have the ability to exhibit necessary conditions for growth and survival, promoting degradation processes of extracellular matrix proteins, such as laminin (LN) and fibronectin (FN). In this study, we evaluated whether treatments, based on free rhodium (II) citrate (Rh2(H2cit)4), maghemite nanoparticles coated with citrate (Magh-cit) and maghemite nanoparticles coated with rhodium (II) citrate (Magh-Rh2(H2cit)4), in murine metastatic breast carcinoma models can modulate the expression of laminin and fibronectin proteins. Synthesized nanoparticles were characterized using X-ray diffraction, transmission electron microscopy, energy dispersive spectroscopy and dynamic light scattering. The expression of FN and LN was assessed using immunohistochemistry and western blotting. The gene expression of FN1 and LAMA1 were evaluated using real-time PCR. The FN1 and LAMA1 transcripts from the Magh-Rh2(H2cit)4 treated group were 95% and 94%, respectively, lower than the control group. Significant reduction in tumor volume for animals treated with Magh-Rh2(H2cit)4 was observed, of about 83%. We witnessed statistically significant reductions of FN and LN expression following treatment with Magh-Rh2(H2cit)4. We have demonstrated that the antitumor effects of Magh-Rh2(H2cit)4 and Rh2(H2cit)4 regulate the expression of FN and LN in metastatic breast tumors.
A Proposal to Stratify the Intermediate-Risk Thyroid Nodules According to the AACE/ACE/AME Guidelines with Ultrasound Features
Authors: Deng Xiao-Hong , Tang Li-Na , Liu Shui-Qing , Li Xiao-Long , He Ya-Ping , Xu Hui-Xiong 
Published: December 2017

AbstractTo propose a risk stratification system for intermediate-risk thyroid nodules (TNs) according to American Association of Clinical Endocrinologists, American College of Endocrinology and Associazione Medici Endocrinologi Medical (AACE/ACE/AME) Guideline with ultrasound (US) features. 1000 patients with 1000 nodules (902 benign nodules and 98 malignant nodules) were included. All the nodules were confirmed with either fine needle aspiration (FNA) cytology and follow-up or histology results after surgery. Univariate analysis and binary multivariate logic regression analysis were applied to analyze the possible risk US features associated with malignancy. Receiver operating characteristic curves (ROC) were drew and compared. Univariate analysis and binary multivariate logistic regression analysis showed that indeterminate hyper-echoic spot (OR = 4.544), slightly ill-defined margin (OR = 2.559), slight hyper-echogenicity (OR = 1.992) and no macro-calcification (OR = 1.921) were risk factors for the intermediate-risk thyroid nodules (TNs). A predicting model was established based on the 4 risk factors. The risk rates of malignancy were 5.7% (26/455) in Stage I, 11.0% (49/445) in Stage II, 23.1% (21/91) in Stage III, 33.3% (3/9) in Stage IV. In conclusion, for the intermediate-risk TNs, special attention should be paid to the TNs with indeterminate hyper-echoic spot, slightly ill-defined margin, slight hyper-echogenicity, or no macro-calcification. The probability of malignancy increased with the number of risk factors increasing.
Complete genome sequencing and genomic characterization of two Escherichia coli strains co-producing MCR-1 and NDM-1 from bloodstream infection
Authors: Zheng Beiwen , Yu Xiao , Xu Hao , Guo Lihua , Zhang Jing , Huang Chen , Shen Ping , Jiang Xiawei , Xiao Yonghong , Li Lanjuan 
Published: December 2017

AbstractWe previously described the discovery of two Escherichia coli isolates (EC1002 and EC2474) co-harbouring mcr-1 and blaNDM-1 genes, which were recovered from bloodstream infection in China. More importantly, these antibiotic resistance genes were located on different plasmids and signaling the potential spread of pandrug-resistant bacteria. Here, the complete genome sequences of both isolates were determined using Pacbio RS II and Illumina HiSeq2000 systems. The genome of EC1002 consists of a 5,177,501 base pair chromosome and four circular plasmids, while the genome of EC2474 consists of a 5,013,813 base pair chromosome and three plasmids. The plasmid replicon type of pEC1002_NDM and pEC2474_NDM were identified as IncA/C2 and IncF, respectively. The genetic environment of blaNDM-1 in this study was similar to blaNDM-carrying plasmids detected in China, although the overall nucleotide identity and query coverage were variable. The plasmid replicon type of pEC1002_MCR and pEC2474_MCR were identified as IncI2 and IncHI2, respectively. Two different genetic strategies for mcr-1 gene spread were observed in this study and blaNDM-1 genes were also found transferred by two different mobile genetic elements in two plasmids. The findings of this study further support that the diversified transfer mechanisms of blaNDM-1 and mcr-1 present in Enterobacteriaceae.
Intensive spa and exercise therapy program for returning to work for low back pain patients: a randomized controlled trial
Authors: Nguyen Christelle , Boutron Isabelle , Rein Christopher , Baron Gabriel , Sanchez Katherine , Palazzo Clémence , Dupeyron Arnaud , Tessier Jean-Max , Coudeyre Emmanuel , Eschalier Bénédicte , Forestier Romain , Roques-Latrille Christian-François , Attal Ygal , Lefèvre-Colau Marie-Martine , Rannou François , Poiraudeau Serge 
Published: December 2017

AbstractWe aimed to determine whether a 5-day intensive inpatient spa and exercise therapy and educational program is more effective than usual care in improving the rate of returning to work at 1 year for patients with subacute and chronic low back pain (LBP) on sick leave for 4 to 24 weeks. We conducted a 12-month randomized controlled trial. LBP patients were assigned to 5-day spa (2 hr/day), exercise (30 min/day) and education (45 min/day) or to usual care. The primary outcome was the percentage of patients returning to work at 1 year after randomization. Secondary outcomes were pain, disability and health-related quality of life at 1 year and number of sick leave days from 6 to 12 months. The projected recruitment was not achieved. Only 88/700 (12.6%) patients planned were enrolled: 45 in the spa therapy group and 43 in the usual care group. At 1 year, returning to work was 56.3% versus 41.9% (OR 1.69 [95% CI 0.60–4.73], p = 0.32) respectively. There was no significant difference for any of the secondary outcomes. However, our study lacked power.
The seven sisters DANCe
Authors: Olivares J. , Moraux E. , Sarro L. M. , Bouy H. , Berihuete A. , Barrado D. , Huelamo N. , Bertin E. , Bouvier J. 
Published: December 2017

Context. Membership analyses of the DANCe and Tycho + DANCe data sets provide the largest and least contaminated sample of Pleiades candidate members to date.
Aims. We aim at reassessing the different proposals for the number surface density of the Pleiades in the light of the new and most complete list of candidate members, and inferring the parameters of the most adequate model.
Methods. We compute the Bayesian evidence and Bayes Factors for variations of the classical radial models. These include elliptical symmetry, and luminosity segregation. As a by-product of the model comparison, we obtain posterior distributions for each set of model parameters.
Results. We find that the model comparison results depend on the spatial extent of the region used for the analysis. For a circle of 11.5 parsecs around the cluster centre (the most homogeneous and complete region), we find no compelling reason to abandon King’s model, although the Generalised King model introduced here has slightly better fitting properties. Furthermore, we find strong evidence against radially symmetric models when compared to the elliptic extensions. Finally, we find that including mass segregation in the form of luminosity segregation in the J band is strongly supported in all our models.
Conclusions. We have put the question of the projected spatial distribution of the Pleiades cluster on a solid probabilistic framework, and inferred its properties using the most exhaustive and least contaminated list of Pleiades candidate members available to date. Our results suggest however that this sample may still lack about 20% of the expected number of cluster members. Therefore, this study should be revised when the completeness and homogeneity of the data can be extended beyond the 11.5 parsecs limit. Such a study will allow for more precise determination of the Pleiades spatial distribution, its tidal radius, ellipticity, number of objects and total mass.
Metastatic Osseous Pain Control: Bone Ablation and Cementoplasty
Authors: Cornelis Francois , Tutton Sean , Filippiadis Dimitrios , Kelekis Alexis 
Published: December 2017

AbstractNociceptive and/or neuropathic pain can be present in all phases of cancer (early and metastatic) and are not adequately treated in 56 to 82.3% of patients. In these patients, radiotherapy achieves overall pain responses (complete and partial responses combined) up to 60 and 61%. On the other hand, nowadays, ablation is included in clinical guidelines for bone metastases and the technique is governed by level I evidence. Depending on the location of the lesion in the peripheral skeleton, either the Mirels scoring or the Harrington (alternatively the Levy) grading system can be used for prophylactic fixation recommendation. As minimally invasive treatment options may be considered in patients with poor clinical status or limited life expectancy, the aim of this review is to detail the techniques proposed so far in the literature and to report the results in terms of safety and efficacy of ablation and cementoplasty (with or without fixation) for bone metastases. Percutaneous image-guided treatments appear as an interesting alternative for localized metastatic lesions of the peripheral skeleton.
Palliative Percutaneous Biliary Interventions in Malignant High Bile Duct Obstruction
Authors: Deipolyi Amy , Covey Anne 
Published: December 2017

AbstractThe optimal palliative intervention for malignant biliary obstruction is internal drainage by placement of a metallic stent. For patients with hilar biliary obstruction or low bile duct obstruction in whom endoscopy is not feasible, a percutaneous transhepatic approach in interventional radiology is preferred. This article reviews the rationale for this approach, periprocedural management, and techniques to optimize stent patency.
Malignant Venous Obstruction: Superior Vena Cava Syndrome and Beyond
Authors: Friedman Tamir , Quencer Keith , Kishore Sirish , Winokur Ronald , Madoff David 
Published: December 2017

AbstractVenous obstruction in the cancer population can result in substantial morbidity and, in extreme cases, mortality. While venous obstruction can be caused by both benign and malignant etiologies in this population, the management of malignant venous obstruction as a palliative measure can be somewhat nuanced with respect to nonprocedural and procedural management, both with respect to treatment of the underlying malignancy as well as treatment of venous hypertension, which may be associated with venous thrombosis. Symptom severity, primary malignancy, functional status, and prognosis are all fundamental to the patient workup and dictate both the timing and extent of endovascular intervention. The morbidity and mortality associated with malignant obstructions of central venous structures, specifically the superior vena cava and inferior vena cava, can be significantly improved with endovascular management in appropriately selected patients. Thus, the pertinent literature regarding the clinical presentation, workup, and endovascular management of malignant central venous obstruction syndromes, with directed attention to superior vena cava syndrome and inferior vena cava syndrome, will be reviewed in this article.
Are Trabecular Metal Cones a Valid Option to Treat Metaphyseal Bone Defects in Complex Primary and Revision Knee Arthroplasty?
Authors: Bonanzinga Tommaso , Gehrke Thorsten , Zahar Akos , Zaffagnini Stefano , Marcacci Maurilio , Haasper Carl 
Published: December 2017

Purpose Metaphyseal bone defects are a challenge in complex primary and revision total knee arthroplasty (TKA). Recently, several studies have been published with promising results about the use of Trabecular Metal (TM) cones to address bone defects. The aim of this study is to review the literature to assess the efficacy of TM cones to address metaphyseal bone loss.
Methods A comprehensive search was performed on PubMed, Medline, CINAHL, Cochrane, Embase, and Google Scholar databases using various combinations of the following keywords: “metaphyseal,” “cones,” “tantalum,” “knee,” and “revision.” Only papers reporting clinical data about the use of trabecular metal cones were included in the analysis. In vitro studies, case reports, surgical technique, or other studies where it was not possible to collect clinical data were excluded. Patients characteristics, details of the surgical procedures, outcome, and complications were collected from each included study.
Results No controlled studies were available in the literature and all the papers were case series. In 16 studies included, the records of 442 patients with 447 implants and 523 TM cones were reported. The mean follow-up was 42 months (range: 5–105) for 360 procedures. Among 437 procedures, 30.4% were septic revisions. The Anderson Orthopaedic Research Institute (AORI) classification was available for 352 defects: 13 type 1, 69 type 2A, 115 type 2B, and 155 type 3. To manage these 352 defects, 360 TM cones were implanted. Intraoperative fractures occurred 13 times (10 femoral/3 tibial), 6 required surgical fixation. The overall infection rate was 7.38%, and the infection rate for the aseptic procedures was 0.99%. An aseptic exchange was performed 13 times, among these procedures two TM cones were loose. Signs of loosening were found just in 1.3% of the 523 TM cones implanted (5 femoral/2 tibial) during 447 procedures.
Conclusion The TM cones are an effective solution to manage bone defects in complex primary and revision TKA at intermediate follow-up. The incidence of complications was low; however, the femoral metaphysis proved to be more susceptible to complications.
Level of Evidence Level IV, systematic review of level IV studies.
Phenotypic and Genotypic Heterogeneity of RRM2B Variants
Authors: Zarrouk-Mahjoub Sinda , Finsterer Josef 
Published: December 2017

Objectives Genotype and phenotype of RRM2B mutation have become increasingly heterogeneous. This review aims at summarizing recent advances concerning the genotypic and phenotypic variability of RRM2B mutations.
Method The review evaluated clinical and instrumental data of 82 patients carrying a mutation in the RRM2B gene reported in 18 publications with regard to onset, frequency, and type of clinical manifestations and genetic findings.
Results The review showed marked variety of clinical manifestations and marked variety of age at onset. Organs predominantly affected in RRM2B mutation carriers are the skeletal muscle, the brain, and the kidneys. Additionally affected may be the eyes, ears, endocrine organs, heart, gastro-intestinal tract, bone marrow, or the peripheral nerves. So far 43 mutations in 81 patients have been reported. Diagnosing RRM2B-associated disease requires demonstration of an appropriate phenotype and a RRM2B mutation. Various clinical manifestations are accessible to various invasive or noninvasive therapeutic measures. The outcome of RRM2B-associated mitochondrial disorders is highly variable ranging from early death to survival into adulthood.
Conclusions Phenotype and genotype in RRM2B mutation carriers are more widespread than anticipated. Particularly in patients with chronic progressive external ophthalmoplegia, ptosis, limb muscle weakness, muscle hypotonia, renal tubulopathy, ataxia, deafness, and lactic acidosis, a RRM2B mutation should be considered.
Embolization of Bleeding Pinna Arteriovenous Malformation after Ligation of the External Carotid Artery: A Therapeutic Challenge
Authors: Kumar Rahul , Goyal Ankur , Bhalla Ashu , Sandip Sonia , Sikka Kapil 
Published: December 2017

AbstractA 25-year-old patient presented with bleeding of right pinna arteriovenous malformation (AVM). There was history of ipsilateral external carotid artery (ECA) ligation 10 years back. Subsequent investigations (ultrasound, magnetic resonance imaging, digital subtraction angiography) showed recruitment of complex collaterals from the ipsilateral subclavian artery and vertebral artery feeding the recurrent nidus. The patient underwent two sessions of endovascular embolization and one session of percutaneous embolization. We wish to highlight the feasibility of antegrade embolization in such cases via collaterals and role of direct percutaneous treatment.
Alkohol ist ein gefährliches Produkt
Authors: John Ulrich 
Published: December 2017

AbstractAlcohol is a hazardous product. Its consumption depends on the society’s drinking norms. These are set by advertising industries on the one hand, and forces of public health and medical services on the other hand. Germany is one of the countries with high alcohol consumption. According to epidemiological evidence, alcohol-attributable diseases include more than 200 diagnostic entities. Medical practitioners may help to reduce alcohol consumption. Data revealed that brief intervention, including computer-generated feedback in medical practice, is efficient in reducing consumption. It relieves the physician of counseling responsibilities.
Compression garments and cerebral blood flow: Influence on cognitive and exercise performance
Authors: Smale Brittany A. , Northey Joseph M. , Smee Disa J. , Versey Nathan G. , Rattray Ben 
Published: December 2017

AbstractThis study aimed to describe the effect of compression garments on middle cerebral artery blood flow velocity (MCAv) in relation to cognitive and exercise performance whilst cycling. In a randomised‐controlled‐cross‐over design, 15 well‐trained male cyclists were recruited to participate in three identical trials wearing loose fitting shorts (control), low‐grade, or medium‐grade compression garments. The protocol involved four 8 min increments of cycling at 30%, 50%, 70%, and 85% maximal power output and a 4 km time‐trial. Participants undertook a cognitive Stroop task at baseline and at the midpoint of each increment. MCAv was monitored with Transcranial Doppler Ultrasonography. Mean arterial pressure (MAP) and partial pressure of end‐tidal CO2 (PetCO2) were measured throughout. MCAv, MAP, PetCO2, and reaction time of the complex Stroop task were influenced by exercise intensity, but not compression garments. Compression garments significantly affected cognitive accuracy in the complex Stroop task such that low‐grade compression appeared to enhance cognitive accuracy in comparison to the control condition at the highest intensity (p = .010). Time‐trial performance did not differ between the control (338.0 ± 17.3 s), low‐grade (338.7 ± 18.7 s), or medium‐grade (342.2 ± 19.3 s) conditions (p = .114). Compression garments did not affect MCAv during exercise or time‐trial performance, but compression may be beneficial for improved cognitive accuracy during high‐intensity exercise. Further research is required to elucidate the potential impact on cognitive performance.
SEGreg: a database for human specifically expressed genes and their regulations in cancer and normal tissue
Authors: Tang Qin , Zhang Qiong , Lv Yao , Miao Ya-Ru , Guo An-Yuan 
Published: December 2017

AbstractHuman specifically expressed genes (SEGs) usually serve as potential biomarkers for disease diagnosis and treatment. However, the regulation underlying their specific expression remains to be revealed. In this study, we constructed SEG regulation database (SEGreg; available at http://bioinfo.life.hust.edu.cn/SEGreg) for showing SEGs and their transcription factors (TFs) and microRNA (miRNA) regulations under different physiological conditions, which include normal tissue, cancer tissue and cell line. In total, SEGreg collected 6387, 1451, 4506 and 5320 SEGs from expression profiles of 34 cancer types and 55 tissues of The Cancer Genome Atlas, Cancer Cell Line Encyclopedia, Human Body Map and Genotype-Tissue Expression databases/projects, respectively. The cancer or tissue corresponding expressed miRNAs and TFs were identified from miRNA and gene expression profiles, and their targets were collected from several public resources. Then the regulatory networks of all SEGs were constructed and integrated into SEGreg. Through a user-friendly interface, users can browse and search SEGreg by gene name, data source, tissue, cancer type and regulators. In summary, SEGreg is a specialized resource to explore SEGs and their regulations, which provides clues to reveal the mechanisms of carcinogenesis and biological processes.
Modeling the rate of HIV testing from repeated binary data amidst potential never-testers
Authors: Rice John D , Johnson Brent A , Strawderman Robert L 
Published: December 2017

SUMMARYMany longitudinal studies with a binary outcome measure involve a fraction of subjects with a homogeneous response profile. In our motivating data set, a study on the rate of human immunodeficiency virus (HIV) self-testing in a population of men who have sex with men (MSM), a substantial proportion of the subjects did not self-test during the follow-up study. The observed data in this context consist of a binary sequence for each subject indicating whether or not that subject experienced any events between consecutive observation time points, so subjects who never self-tested were observed to have a response vector consisting entirely of zeros. Conventional longitudinal analysis is not equipped to handle questions regarding the rate of events (as opposed to the odds, as in the classical logistic regression model). With the exception of discrete mixture models, such methods are also not equipped to handle settings in which there may exist a group of subjects for whom no events will ever occur, i.e. a so-called “never-responder” group. In this article, we model the observed data assuming that events occur according to some unobserved continuous-time stochastic process. In particular, we consider the underlying subject-specific processes to be Poisson conditional on some unobserved frailty, leading to a natural focus on modeling event rates. Specifically, we propose to use the power variance function (PVF) family of frailty distributions, which contains both the gamma and inverse Gaussian distributions as special cases and allows for the existence of a class of subjects having zero frailty. We generalize a computational algorithm developed for a log-gamma random intercept model (Conaway, 1990. A random effects model for binary data. Biometrics46, 317–328) to compute the exact marginal likelihood, which is then maximized to obtain estimates of model parameters. We conduct simulation studies, exploring the performance of the proposed method in comparison with competitors. Applying the PVF as well as a Gaussian random intercept model and a corresponding discrete mixture model to our motivating data set, we conclude that the group assigned to receive follow-up messages via SMS was self-testing at a significantly lower rate than the control group, but that there is no evidence to support the existence of a group of never-testers.