bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced DICER1 Expression Bestows Rheumatoid Arthritis Synoviocytes Proinflammatory Properties and Resistance to Apoptotic Stimuli
Authors: Alsaleh Ghada , Nehmar Ramzi , Blüml Stephan , Schleiss Cédric , Ostermann Eleonore , Dillenseger Jean‐Philippe , Sayeh Amira , Choquet Philippe , Dembele Doulaye , Francois Antoine , Salmon Jean‐Hugues , Paul Nicodème , Schabbauer Gernot , Bierry Guillaume , Meyer Alain , Gottenberg Jacques‐Eric , Haas Gabrielle , Pfeffer Sebastien , Vallat Laurent , Sibilia Jean , Bahram Seiamak , Georgel Philippe 
Published: February 2016

ObjectiveWhile the regulatory role of individual microRNAs (miRNAs) in rheumatoid arthritis (RA) is well established, the role of DICER1 in the pathogenesis of the disease has not yet been investigated. The purpose of this study was to analyze the expression of factors involved in miRNA biogenesis in fibroblast‐like synoviocytes (FLS) from RA patients and to monitor the arthritis triggered by K/BxN serum transfer in mice deficient in the Dicer gene (Dicerd/d).MethodsThe expression of genes and precursor miRNAs was quantified by quantitative reverse transcription–polymerase chain reaction (qRT‐PCR). MicroRNA macroarray profiling was monitored by qRT‐PCR. Cytokines were quantified by enzyme‐linked immunosorbent assay. Experimental arthritis in mice was achieved by the transfer of serum from K/BxN donors. Apoptosis was quantified using an enzyme‐linked immunosorbent assay.ResultsWe found decreased DICER1 and mature miRNA expression in synovial fibroblasts from RA patients. These cells were hyperresponsive to lipopolysaccharide, as evidenced by their increased interleukin‐6 secretion upon stimulation. Experimental serum‐transfer arthritis in Dicerd/d mice confirmed that an unbalanced biogenesis of miRNAs correlated with an enhanced inflammatory response. Synoviocytes from both RA patients and Dicerd/d mice exhibited increased resistance to apoptotic stimuli.ConclusionThe findings of this study further substantiate the important role of DICER1 in the maintenance of homeostasis and the regulation of inflammatory responses.
Facile Synthesis of Porous Dendritic Bimetallic Platinum–Nickel Nanocrystals as Efficient Catalysts for the Oxygen Reduction Reaction
Authors: Eid Kamel , Wang Hongjing , Malgras Victor , Alothman Zeid Abdullah , Yamauchi Yusuke , Wang Liang 
Published: February 2016

AbstractCertain bimetallic nanocrystals (NCs) possess promising catalytic properties for electrochemical energy conversion. Herein, we report a facile method for the one‐step synthesis of porous dendritic PtNi NCs in aqueous solution at room temperature that contrasts with the traditional multistep thermal decomposition approach. The dendritic PtNi NCs assembled by interconnected arms are efficient catalysts for the oxygen reduction reaction. This direct and efficient method is favorable for the up‐scaled synthesis of active catalysts used in electrochemical applications.
Interaction between MTHFR 677C>T and periconceptional folic acid supplementation in the risk of Hypospadias
Authors: Dokter Elisabeth M.J. , van Rooij Iris A.L.M. , Wijers Charlotte H.W. , Groothuismink Johanne M. , van der Biezen Jan Jaap , Feitz Wout F.J. , Roeleveld Nel , van der Zanden Loes F.M. 
Published: February 2016

BackgroundHypospadias is a congenital malformation with both environmental factors and genetic predisposition involved in the pathogenesis. The role of maternal periconceptional folic acid supplement use in the development of hypospadias is unclear. As folate levels may also be influenced by the C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene, we hypothesize that a gene–environment interaction between this polymorphism and folic acid use is involved in the etiology of hypospadias.MethodsWe conducted a case–control study among 855 hypospadias cases and 713 population‐based controls from the AGORA data‐ and biobank. Folic acid supplement use was derived from maternal questionnaires and infant and maternal DNA was used to determine the MTHFR C677T polymorphism using Taqman assays. We performed separate analyses for different hypospadias phenotypes (anterior/middle/posterior).ResultsHypospadias was neither associated with folic acid use or the MTHFR C677T polymorphism, nor with their interaction. However, we did find an association with middle hypospadias when no supplements were used (odds ratio = 1.6; 95% confidence interval, 1.1–2.4), especially in infants carrying the CT/TT genotype (odds ratio = 2.5; 95% confidence interval, 1.4–4.7). In addition, more infants with these genotypes seemed to have posterior hypospadias, regardless of folic acid use.ConclusionOur study does not suggest a major role for folic acid supplements or the MTHFR C677T polymorphism in the etiology of hypospadias in general, but not using folic acid and/or carrying the MTHFR C677T polymorphism may be associated with middle and posterior hypospadias. Therefore, we stress the importance of studying gene–environment interactions preferably in stratified analyses for different hypospadias phenotypes. Birth Defects Research (Part A) 106:275–284, 2016. © 2016 Wiley Periodicals, Inc.
A novel approach to predict cetuximab‐induced hypersensitivity reaction: detection of drug‐specific IgE on basophils
Authors: Iwamoto Takuya , Okamoto Akiharu , Ishinaga Hajime , Shimizu Kasumi , Gayle Alberto A. , Arai Naoya , Takeuchi Kazuhiko , Okuda Masahiro 
Published: February 2016

AbstractCetuximab is remarkable for the relatively high rate and severity of hypersensitivity reactions (HR) being reported in the literature. Screening for cetuximab‐specific IgE in serum via immunoassay has been found to be useful in preventing HR; however, these tests are known to have a low positive predictive rate. In an attempt to remedy this, we evaluated the interaction between cetuximab and IgE on basophils for predicting severe cetuximab‐induced HR. Twelve head and neck cancer patients were enrolled in this single‐institution study: four with a history of cetuximab‐induced HR and eight with no such history. Cetuximab‐specific and galactose‐α‐1,3‐galactose (α‐gal) specific IgEs in serum were measured in vitro using an enzyme‐linked immunosorbent assay (ELISA). IgE‐cetuximab binding on basophils was also analyzed to evaluate the decrease in cetuximab molecules on basophils after dissociation of IgE from FcεRI. The positive predictive value associated with the presence of cetuximab‐ or α‐gal‐specific IgE in serum was found to be only 0.67, whereas the negative predictive value was 1.00. On the other hand, in all four patients who developed HR, the cetuximab molecules on basophils were decreased significantly due to the dissociation of IgE from basophils (P < 0.05). However, this was not the case in patients who did not develop HR. In conclusion, our results strongly imply that the IgE‐cetuximab interaction on basophils may be key to developing improved methods for predicting severe cetuximab‐induced HR.
The Essential Oil Compositions of Ocimum basilicum from Three Different Regions: Nepal, Tajikistan, and Yemen
Authors: Sharopov Farukh S. , Satyal Prabodh , Ali Nasser A. Awadh , Pokharel Suraj , Zhang Hanjing , Wink Michael , Kukaniev Muhammadsho A. , Setzer William N. 
Published: February 2016

AbstractThe aerial parts of Ocimum basilicum L. were collected from four different geographical locations, Sindhuli and Biratnagar (Nepal), Chormaghzak village (Tajikistan), and Sana'a (Yemen). The essential oils were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry. A cluster analysis of 179 essential oil compositions revealed six major chemotypes: Linalool, eugenol, estragole, methyl eugenol, 1,8‐cineole, and geraniol. All four of the basil oils in this study were of the linalool‐rich variety. Some of the basil oils were screened for bioactivity including antimicrobial, cytotoxicity in human cancer cells, brine shrimp lethality, nematicidal, larvicidal, insecticidal, and antioxidant. The basil oils in this study were not notably antibacterial, cytotoxic, antioxidant, nor nematicidal, but were active in the brine shrimp lethality test, and did show larvicidal and insecticidal activities.
Bioprospecting Chemical Diversity and Bioactivity in a Marine Derived Aspergillus terreus
Authors: Adpressa Donovon A. , Loesgen Sandra 
Published: February 2016

AbstractA comparative metabolomic study of a marine derived fungus (Aspergillus terreus) grown under various culture conditions is presented. The fungus was grown in eleven different culture conditions using solid agar, broth cultures, or grain based media (OSMAC). Multivariate analysis of LC/MS data from the organic extracts revealed drastic differences in the metabolic profiles and guided our subsequent isolation efforts. The compound 7‐desmethylcitreoviridin was isolated and identified, and is fully described for the first time. In addition, 16 known fungal metabolites were also isolated and identified. All compounds were elucidated by detailed spectroscopic analysis and tested for antibacterial activities against five human pathogens and tested for cytotoxicity. This study demonstrates that LC/MS based multivariate analysis provides a simple yet powerful tool to analyze the metabolome of a single fungal strain grown under various conditions. This approach allows environmentally‐induced changes in metabolite expression to be rapidly visualized, and uses these differences to guide the discovery of new bioactive molecules.
Distance Regulated Vesicle Fusion and Docking Mediated by β‐Peptide Nucleic Acid SNARE Protein Analogues
Authors: Sadek Muheeb , Berndt Daniel , Milovanovic Dragomir , Jahn Reinhard , Diederichsen Ulf 
Published: February 2016

AbstractArtificial SNARE analogues derived from SNARE proteins, which mediate synaptic membrane fusion, are of interest. They mimic the tetrameric α‐helix bundle of the SNARE motif with various bio‐oligomer recognition units. Interaction between complementary oligomers linked to the respective membrane by lipid or peptide anchors leads to proximity of vesicles and to fusion of lipid bilayers. β‐Peptide nucleic acids were introduced as hybrid oligomers with the native SNARE protein transmembrane/linker sequence, in order to evaluate a fusion system that allows distance tuning of approaching membranes. Formation of a four‐base pair β‐PNA double strand with 20 Å length is sufficient for vesicle membrane fusion. Elongation of the recognition β‐PNA duplex in the linker region yielded a 40 Å β‐peptide duplex and provided a vesicle–vesicle distance that only supported hemifusion of vesicle membranes.
Synchronous brain and intravascular B‐cell lymphoma after remission of an adult hemophagocytic syndrome
Authors: Fonseca Válter R. , Espada Eduardo , Geraldes Ruth , Ortiz Santiago , Sousa Rita , Lopez Dolores , Silva Marisa Teixeira , Victorino Rui 
Published: February 2016

Key Clinical MessageHemophagocytic lymphohistiocytosis (HLH) should be considered in the differential diagnosis of adult patients with white matter disease. Brain involvement can be life‐threatening and should prompt aggressive therapy. Even after HLH remission, the possibility of subsequent deterioration due to emergence of an aggressive intravascular lymphoma is highlighted here.
Computational Insight on CO2 Fixation to Produce Styrene Carbonate Assisted by a Single‐Center Aluminum(III) Catalyst and Quaternary Ammonium Salts
Authors: Butera Valeria , Russo Nino , Cosentino Ugo , Greco Claudio , Moro Giorgio , Pitea Demetrio , Sicilia Emilia 
Published: February 2016

AbstractDFT calculations were used to investigate the cycloaddition reaction of CO2 to styrene oxide for the formation of styrene carbonate. The uncatalyzed process alongside the reactions assisted by tetrabutylammonium bromide (TBAB), the novel nonsymmetrical single‐center aluminum(III) salen–acac hybrid complex (salenac) (Al1cat) and the binary Al1cat/TBAB system were all investigated and for all of them the optimized structures, rate‐determining steps, and lowest energy barrier reaction pathways were intercepted for both gas‐phase and solvent environments. For the catalyzed systems, the reaction mechanism consists of three key elementary steps: 1) epoxy ring opening; 2) CO2 electrophilic attack and 3) intramolecular cyclization. In the presence of Al1cat, the central metal of the catalyst coordinates with an oxygen atom of the epoxide, activating it towards a nucleophilic attack by the halide. An oxyanion species is formed that affords the corresponding cyclic carbonate after reaction with CO2. Our results provide important hints on the cycloaddition of CO2 and epoxides promoted by nonsymmetrical aluminum complex containing a single metal center, and can satisfactorily explain the previous experimental observations allowing the development of more efficient catalysts for organic carbonate production.
Perovskite LaNiO3 Nanocrystals inside SBA‐15 Silica: High Stability and Anti‐Coking Performance in the Pre‐Reforming of Liquefied Petroleum Gas at a Low Steam‐to‐Carbon Molar Ratio
Authors: Hu Yong , Wang Xueguang , Tan Mingwu , Zou Xiujing , Ding Weizhong , Lu Xionggang 
Published: February 2016

AbstractA high loading of LaNiO3 perovskite nanocrystals (83 wt %) was successfully incorporated inside the ordered mesoporous framework of SBA‐15 silica by a citric acid assisted impregnation method. The obtained LaNiO3/SBA‐15 material produced both La2O3 nanoparticles and metal Ni nanoparticles with intimate contact and highly dispersed in the mesoporous SBA‐15 framework upon reduction with H2, and it showed high activity, high stability, and good anti‐coking ability for the low‐temperature steam reforming of liquefied petroleum gas at a low steam/carbon ratio of 1.0. The excellent performance of the LaNiO3/SBA‐15 material was attributed to the formation of synergistic active sites in the interfacial area, which consisted of metal Ni and La2O2CO3 species, and the confinement effect of the mesoporous structure on the Ni and La2O3 species.
Mechanism of the Accelerated Reduction of an Oxidized Metal Catalyst under Electric Discharge
Authors: Kim Taegyu , Lee Dae Hoon , Jo Sungkwon , Pyun Sung Hyun , Kim Kwan‐Tae , Song Young‐Hoon 
Published: February 2016

AbstractThe front cover artwork for Issue 04/2016 is provided by the Korea Institute of Machinery and Materials and Chosun University. The image shows a novel reaction pathway for the reduction of metal oxide under electric discharge conditions where activated intermediate species desorb without further surface reaction and react with gas‐phase radicals. See the Communication itself at http://dx.doi.org/10.1002/cctc.201500865.
Daldionin, an Unprecedented Binaphthyl Derivative, and Diverse Polyketide Congeners from a Fungal Orchid Endophyte
Authors: Barnes Emma C. , Jumpathong Juangjun , Lumyong Saisamorn , Voigt Kerstin , Hertweck Christian 
Published: February 2016

AbstractThailand possesses a rich diversity of orchid species that, in turn, live in symbiosis with a wide variety of fungi. Such endophytes have the potential to produce secondary metabolites with bioactivity against orchid and/or human pathogens. The orchid‐associated fungal strain Daldinia eschscholtzii was found to produce a diverse range of aromatic polyketides including the new naphthalene derivatives daldionin, nodulones B and C, and daldinones F and G along with eight known compounds. Daldionin possesses an unprecedented oxane‐linked binaphthyl ring system. These compounds demonstrate the high diversity of structural variations that are constructed during fungal biosynthesis, and the results include important observations concerning the biosynthesis of binaphthyl derivatives. Daldionin was found to have weak antiproliferative activity against HUVEC and K‐562 cell lines. All but one of the isolated compounds showed moderate antimicrobial activity towards at least one of the four tested microbial strains.
Trianguleniums as Optical Probes for G‐Quadruplexes: A Photophysical, Electrochemical, and Computational Study
Authors: Shivalingam Arun , Vyšniauskas Aurimas , Albrecht Tim , White Andrew J. P. , Kuimova Marina K. , Vilar Ramon 
Published: February 2016

AbstractNucleic acids can adopt non‐duplex topologies, such as G‐quadruplexes in vitro. Yet it has been challenging to establish their existence and function in vivo due to a lack of suitable tools. Recently, we identified the triangulenium compound DAOTA‐M2 as a unique fluorescence probe for such studies. This probe's emission lifetime is highly dependent on the topology of the DNA it interacts with opening up the possibility of carrying out live‐cell imaging studies. Herein, we describe the origin of its fluorescence selectivity for G‐quadruplexes. Cyclic voltammetry predicts that the appended morpholino groups can act as intra‐ molecular photo‐induced electron transfer (PET) quenchers. Photophysical studies show that a delicate balance between this effect and inter‐molecular PET with nucleobases is key to the overall fluorescence enhancement observed upon nucleic acid binding. We utilised computational modelling to demonstrate a conformational dependence of intra‐molecular PET. Finally, we performed orthogonal studies with a triangulenium compound, in which the morpholino groups were removed, and demonstrated that this change inverts triangulenium fluorescence selectivity from G‐quadruplex to duplex DNA, thus highlighting the importance of fine tuning the molecular structure not only for target affinity, but also for fluorescence response.
One‐Pot Synthesis of Pomegranate‐Structured Fe3O4/Carbon Nanospheres‐Doped Graphene Aerogel for High‐Rate Lithium Ion Batteries
Authors: He Dafang , Li Lixian , Bai Fengjuan , Zha Chenyang , Shen Liming , Kung Harold H. , Bao Ningzhong 
Published: February 2016

AbstractA unique hierarchically nanostructured composite of iron oxide/carbon (Fe3O4/C) nanospheres‐doped three‐dimensional (3D) graphene aerogel has been fabricated by a one‐pot hydrothermal strategy. In this novel nanostructured composite aerogel, uniform Fe3O4 nanocrystals (5–10 nm) are individually embedded in carbon nanospheres (ca. 50 nm) forming a pomegranate‐like structure. The carbon matrix suppresses the aggregation of Fe3O4 nanocrystals, avoids direct exposure of the encapsulated Fe3O4 to the electrolyte, and buffers the volume expansion. Meanwhile, the interconnected 3D graphene aerogel further serves to reinforce the structure of the Fe3O4/C nanospheres and enhances the electrical conductivity of the overall electrode. Therefore, the carbon matrix and the interconnected graphene network entrap the Fe3O4 nanocrystals such that their electrochemical function is retained even after fracture. This novel hierarchical aerogel structure delivers a long‐term stability of 634 mA h g−1 over 1000 cycles at a high current density of 6 A g−1 (7 C), and an excellent rate capability of 413 mA h g−1 at 10 A g−1 (11 C), thus exhibiting great potential as an anode composite structure for durable high‐rate lithium‐ion batteries.
Computational Study of Formic Acid Dehydrogenation Catalyzed by AlIII–Bis(imino)pyridine
Authors: Lu Qian‐Qian , Yu Hai‐Zhu , Fu Yao 
Published: February 2016

AbstractThe mechanism of formic acid dehydrogenation catalyzed by the bis(imino)pyridine‐ligated aluminum hydride complex (PDI2−)Al(THF)H (PDI=bis(imino)pyridine) was studied by density functional theory calculations. The overall transformation is composed of two stages: catalyst activation and the catalytic cycle. The catalyst activation begins with O−H bond cleavage of HCOOH promoted by aluminum–ligand cooperation, followed by HCOOH‐assisted Al−H bond cleavage, and protonation of the imine carbon atom of the bis(imino)pyridine ligand. The resultant doubly protonated complex (H,HPDI)Al(OOCH)3 is the active catalyst for formic acid dehydrogenation. Given this, the catalytic cycle includes β‐hydride elimination of (H,HPDI)Al(OOCH)3 to produce CO2, and the formed (H,HPDI)Al(OOCH)2H mediates HCOOH to release H2.
Halide‐Free Synthesis of Hydrochalcogenide Ionic Liquids of the Type [Cation][HE] (E=S, Se, Te)
Authors: Finger Lars H. , Sundermeyer Jörg 
Published: February 2016

AbstractWe present the synthesis and thorough characterization of ionic liquids and organic salts based on hydrochalcogenide HE− (E=S, Se, Te) anions. Our approach is based on halide‐, metal‐, and water‐free decarboxylation of methylcarbonate precursors under acidic conditions, resulting from the easily dissociating reagents H2E. The compounds were characterized by elemental analysis, multinuclear NMR spectroscopy, thermal and single‐crystal XRD analyses. The hydrosulfide salts were investigated with respect to their ability to dissolve elemental sulfur in varying stoichiometry. Thus‐prepared polysulfide ILs were also analyzed by UV/Vis spectroscopy and cyclic voltammetry.
Bis‐tert‐Alcohol‐Functionalized Crown‐6‐Calix[4]arene: An Organic Promoter for Nucleophilic Fluorination
Authors: Jadhav Vinod H. , Choi Wonsil , Lee Sung‐Sik , Lee Sungyul , Kim Dong Wook 
Published: February 2016

AbstractA bis‐tert‐alcohol‐functionalized crown‐6‐calix[4]arene (BACCA) was designed and prepared as a multifunctional organic promoter for nucleophilic fluorinations with CsF. By formation of a CsF/BACCA complex, BACCA could release a significantly active and selective fluoride source for SN2 fluorination reactions. The origin of the promoting effects of BACCA was studied by quantum chemical methods. The role of BACCA was revealed to be separation of the metal fluoride to a large distance (>8 Å), thereby producing an essentially “free” F−. The synergistic actions of the crown‐6‐calix[4]arene subunit (whose O atoms coordinate the counter‐cation Cs+) and the terminal tert‐alcohol OH groups (forming controlled hydrogen bonds with F−) of BACCA led to tremendous efficiency in SN2 fluorination of base‐sensitive substrates.
Influence of Equilibration Time in Solution on the Inclusion/Exclusion Topology Ratio of Host–Guest Complexes Probed by Ion Mobility and Collision‐Induced Dissociation
Authors: Carroy Glenn , Daxhelet Charlotte , Lemaur Vincent , De Winter Julien , De Pauw Edwin , Cornil Jérôme , Gerbaux Pascal 
Published: February 2016

AbstractHost–guest complexes are formed by the creation of multiple noncovalent bonds between a large molecule (the host) and smaller molecule(s) or ion(s) (the guest(s)). Ion‐mobility separation coupled with mass spectrometry nowadays represents an ideal tool to assess whether the host–guest complexes, when transferred to the gas phase upon electrospray ionization, possess an exclusion or inclusion nature. Nevertheless, the influence of the solution conditions on the nature of the observed gas‐phase ions is often not considered. In the specific case of inclusion complexes, kinetic considerations must be taken into account beside thermodynamics; the guest ingression within the host cavity can be characterized by slow kinetics, which makes the complexation reaction kinetically driven on the timescale of the experiment. This is particularly the case for the cucurbituril family of macrocyclic host molecules. Herein, we selected para‐phenylenediamine and cucurbit[6]uril as a model system to demonstrate, by means of ion mobility and collision‐induced dissociation measurements, that the inclusion/exclusion topology ratio varies as a function of the equilibration time in solution prior to the electrospray process.