bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remote Loading of Small‐Molecule Therapeutics into Cholesterol‐Enriched Cell‐Membrane‐Derived Vesicles
Authors: Zhang Xinxin , Angsantikul Pavimol , Ying Man , Zhuang Jia , Zhang Qiangzhe , Wei Xiaoli , Jiang Yao , Zhang Yue , Dehaini Diana , Chen Mengchun , Chen Yijie , Gao Weiwei , Fang Ronnie H. , Zhang Liangfang 
Published: September 2017

AbstractThe increasing popularity of biomimetic design principles in nanomedicine has led to therapeutic platforms with enhanced performance and biocompatibility. This includes the use of naturally derived cell membranes, which can bestow nanocarriers with cell‐specific functionalities. Herein, we report on a strategy enabling efficient encapsulation of drugs via remote loading into membrane vesicles derived from red blood cells. This is accomplished by supplementing the membrane with additional cholesterol, stabilizing the nanostructure and facilitating the retention of a pH gradient. We demonstrate the loading of two model drugs: the chemotherapeutic doxorubicin and the antibiotic vancomycin. The therapeutic implications of these natural, remote‐loaded nanoformulations are studied both in vitro and in vivo using animal disease models. Ultimately, this approach could be used to design new biomimetic nanoformulations with higher efficacy and improved safety profiles.
Single Turnover at Molecular Polymerization Catalysts Reveals Spatiotemporally Resolved Reactions
Authors: Easter Quinn T. , Blum Suzanne A. 
Published: September 2017

AbstractMultiple active individual molecular ruthenium catalysts have been pinpointed within growing polynorbornene, thereby revealing information on the reaction dynamics and location that is unavailable through traditional ensemble experiments. This is the first single‐turnover imaging of a molecular catalyst by fluorescence microscopy and allows detection of individual monomer reactions at an industrially important molecular ruthenium ring‐opening metathesis polymerization (ROMP) catalyst under synthetically relevant conditions (e.g. unmodified industrial catalyst, ambient pressure, condensed phase, ca. 0.03 m monomer). These results further establish the key fundamentals of this imaging technique for characterizing the reactivity and location of active molecular catalysts even when they are the minor components.
Evidence for Opportunity Cost Neglect in the Poor
Authors: Plantinga Arnoud , Krijnen Job M.T. , Zeelenberg Marcel , Breugelmans Seger M. 
Published: September 2017

AbstractPeople often neglect opportunity costs: They do not fully take into account forgone alternatives outside of a particular choice set. Several scholars have suggested that poor people should be more likely to spontaneously consider opportunity costs, because budget constraints should lead to an increased focus on trade‐offs. We did not find support for this hypothesis in five high‐powered experiments (total N = 2325). The experiments used different products (both material and experiential) with both high and low prices (from $8.50 to $249.99) and different methods of reminding participants of opportunity costs. High‐income and low‐income participants showed an equally strong decrease in willingness to buy when reminded of opportunity costs, implying that both the rich and the poor neglect opportunity costs. © 2017 The Authors Journal of Behavioral Decision Making Published by John Wiley & Sons Ltd.
Use of endoscopy to determine the resection margin during laparoscopic gastrectomy for cancer
Authors: Kawakatsu S , Ohashi M , Hiki N , Nunobe S , Nagino M , Sano T 
Published: September 2017

Abstract

Background
It can be difficult to determine the transection line during totally laparoscopic surgery for early gastric cancer owing to lack of tactile feedback. This retrospective cohort study aimed to assess the role of intraoperative endoscopy in determining the resection margin in totally laparoscopic gastrectomy.

Methods
Consecutive patients with histologically confirmed gastric cancer who underwent laparoscopic gastrectomy between March 2012 and July 2015 were eligible. Preoperative placement of marking clips and intraoperative endoscopy were performed to determine the resection margin. Frozen-section analyses were also performed to confirm the absence of cancer cells at the surgical margin. Success was defined as the proportion of specimens with all clips present and by the proportion of resections with a negative surgical margin following initial transection.

Results
Total laparoscopic gastrectomy with intraoperative endoscopy was performed in 522 patients; a total of 662 surgical margins were analysed. The overall success rate was 99·8 per cent (661 of 662 margins). The success rate of achieving a negative surgical margin during the initial transection was 98·9 per cent (550 of 556 margins).

Conclusion
Preoperative placement of marking clips and intraoperative endoscopy is helpful in the determination of a safe surgical margin in patients with gastric cancer who undergo laparoscopic gastrectomy.
Fluorescent Probe DCVJ Shows High Sensitivity for Characterization of Amyloid β‐Peptide Early in the Lag Phase
Authors: Nagarajan Sureshbabu , Lapidus Lisa J. 
Published: September 2017

AbstractThe aggregation of intrinsically disordered and misfolded proteins in the form of oligomers and fibrils plays a crucial role in a number of neurological and neurodegenerative diseases. Currently, most probes and biophysical techniques that detect and characterize fibrils at high resolution fail to show sensitivity and binding for oligomers. Here, we show that 9‐(dicyano‐vinyl)julolidine (DCVJ), a class of molecular rotor, binds amyloid beta (Aβ) early aggregates, and we report the kinetics as well as packing of the oligomer formation. The binding of DCVJ to Aβ40 increased its emission intensity with time at 510 nm and produced a second excimer peak at 575 nm. However, DCVJ did not bind to the prefibrillar aggregates of Aβ42, which indicated that the oligomers formed by Aβ40 and Aβ42 were not the same. The F4C F19W mutant of Aβ40, which did not form fibrils, also bound DCVJ, but the emission spectral profile varied from that of the wild‐type (WT). Atomic force microscopy images of WT Aβ40, the F4C F19W mutant, and Aβ42 oligomers displayed differences in size and shape, confirming the difference in their DCVJ spectra. The effect of epigallocatechin‐3‐gallate (EGCG) on the reduction of Aβ42 fibrils was also observed with finer detail than with other techniques. The results of this study show that DCVJ detects early aggregates and provides valuable information regarding the oligomer kinetics, packing, and mechanism of formation.
Modulating the Physical and Electronic Properties over Positional Isomerism: The Dispirofluorene–Dihydroindacenodithiophene (DSF‐IDT) Family
Authors: Peltier Jean‐David , Heinrich Benoît , Donnio Bertrand , Jeannin Olivier , Rault‐Berthelot Joëlle , Poriel Cyril 
Published: September 2017

AbstractWe report the first studies on the intrinsic properties of a meta‐substituted dihydroindacenodithienyl fragment and more generally the strong impact of positional isomerism on dihydroindacenodithiophene derivatives. The influence of the para and meta linkages has notably been highlighted not only for the electronic properties in solution (electrochemical properties, anodic polymerization, HOMO/LUMO energy levels, optical transitions, fluorescence spectra) but also on the physical properties in the solid state (molecular organization, crystallinity, and phase transitions). The positional isomerism hence appears to be a very efficient tool to drastically tune the properties of dihydroindacenodithiophene derivatives.
Determination of the Absolute Configuration of (−)‐Hydroxynitrilaphos and Related Biosynthetic Questions
Authors: Pallitsch Katharina , Happl Barbara , Stieger Christian 
Published: September 2017

AbstractInvited for the cover of this issue is the group of Katharina Pallitsch at the University of Vienna. The image depicts an abstract rendition of the still unknown mechanism of hydroxynitrilaphos biosynthesis as an example for the treasure trove of unusual enzymology involved in phosphonate metabolism. Read the full text of the article at 10.1002/chem.201702904.
Cognitive performance and emotion are indifferent to ambient color
Authors: von Castell Christoph , Stelzmann Daniela , Oberfeld Daniel , Welsch Robin , Hecht Heiko 
Published: September 2017

AbstractFolklore has it that ambient color has the power to relax or arouse the observer and enhance performance when executing cognitive tasks. We picked a number of commercially available colors that allegedly have the power to alter cognitive performance and the emotional state, and exposed subjects to them while solving a battery of cognitive tasks. The colors were “Cool Down Pink”, which is said to produce relaxing effects and reduce effort, “Energy Red”, allegedly enhancing performance via increased arousal, “Relaxing Blue”, which is said to enhance attention and concentration, as well as white as a control. In a between‐subjects design, a total of 170 high school students carried out five tasks (number series completion, mental rotation, and memory for word categories, word pairs, and geometrical figures) while exposed to one of the four colors. The emotional state of the subjects was measured before the beginning and at the end of the experiment. The ambient colors did not have the predicted effects, neither on cognitive performance nor on the emotional state of the participants.
Paper‐based Electrochemical Devices Coupled to External Graphene‐Cu Nanoparticles Modified Solid Electrode through Meniscus Configuration and their Use in Biological Analysis
Authors: Petroni Jacqueline Marques , Lucca Bruno Gabriel , da Silva Júnior Luiz Carlos , Barbosa Alves Diego Carvalho , Souza Ferreira Valdir 
Published: September 2017

AbstractThis paper demonstrates, for the first time, the use of paper‐based electrochemical devices coupled to external solid working electrodes. The paper‐based electrochemical cells were fabricated using inexpensive and largely available office paper, according to a simple protocol that consists on the creation of hydrophobic barriers using paraffinized paper and preheated metal stamp. The counter and reference electrodes were integrated to the paper platform through the deposition of carbon and silver inks, respectively. The electrochemical paper analytical device (ePAD) was coupled to external glassy carbon rod electrode modified with reduced graphene oxide doped with Cu nanoparticles through meniscus configuration. The analytical usefulness of this electrochemical approach was demonstrated through the simultaneous determination of paracetamol and caffeine in biological samples. The analytes were successfully quantified in real urine samples and limits of detection of 24.6 nM (paracetamol) and 36.1 nM (caffeine) were obtained. The paper platform showed good stability (RSD of 1.07 % for the peak currents and 1.43 % for the peak potentials) and satisfactory performance. The use of solid electrodes coupled to paper electrochemical devices, firstly demonstrated here, opens new possibilities for the utilization of ePADs in electrochemistry and electroanalytical chemistry and offers advantages such as the extremely reduced consumption of reagents and the minimal generation of wastes.
Spatiotemporal variations of suspended sediment transport in the upstream and midstream of the Yarlung Tsangpo River (the upper Brahmaputra), China
Authors: Shi Xiaonan , Zhang Fan , Lu Xixi , Wang Zhaoyin , Gong Tongliang , Wang Guanxing , Zhang Hongbo 
Published: September 2017

AbstractThe Yarlung Tsangpo River, which flows from west to east across the southern part of the Tibetan Plateau, is the longest river on the plateau and an important center for human habitation in Tibet. Suspended sediment in the river can be used as an important proxy for evaluating regional soil erosion and ecological and environmental conditions. However, sediment transport in the river is rarely reported due to data scarcity. Results from this study based on a daily dataset of 3 years from four main stream gauging stations confirmed the existence of great spatiotemporal variability in suspended sediment transport in the Yarlung Tsangpo River, under interactions of monsoon climate and topographical variability. Temporally, sediment transport or deposition mainly occurred during the summer months from July to September, accounting for 79% to 93% of annual gross sediment load. This coincided with the rainy season from June to August that accounted for 51% to 80% of annual gross precipitation and the flood period from July to September that accounted for approximately 60% of annual gross discharge. The highest specific sediment yield of 177.6 t/km2/yr occurred in the upper midstream with the highest erosion intensity. The lower midstream was dominated by deposition, trapping approximately 40% of total sediment input from its upstream area. Sediment load transported to the midstream terminus was 10.43 Mt/yr with a basin average specific sediment yield of 54 t/km2/yr. Comparison with other plateau‐originated rivers like the upper Yellow River, the upper Yangtze River, the upper Indus River, and the Mekong River indicated that sediment contribution from the studied area was very low. The results provided fundamental information for future studies on soil and water conservation and for the river basin management. Copyright © 2017 John Wiley & Sons, Ltd.
Efficient computation of two‐dimensional steady free‐surface flows
Authors: Pethiyagoda Ravindra , Moroney Timothy J. , McCue Scott W. 
Published: September 2017

SummaryWe consider a family of steady free‐surface flow problems in two dimensions, concentrating on the effect of nonlinearity on the train of gravity waves that appear downstream of a disturbance. By exploiting standard complex variable techniques, these problems are formulated in terms of a coupled system of Bernoulli equation and an integral equation. When applying a numerical collocation scheme, the Jacobian for the system is dense, as the integral equation forces each of the algebraic equations to depend on each of the unknowns. We present here a strategy for overcoming this challenge, which leads to a numerical scheme that is much more efficient than what is normally used for these types of problems, allowing for many more grid points over the free surface. In particular, we provide a simple recipe for constructing a sparse approximation to the Jacobian that is used as a preconditioner in a Jacobian‐free Newton‐Krylov method for solving the nonlinear system. We use this approach to compute numerical results for a variety of prototype problems including flows past pressure distributions, a surface‐piercing object and bottom topographies.
Breast cancer risk and germline genomic profiling of women with neurofibromatosis type 1 who developed breast cancer
Authors: Wang Xia , Teer Jamie K. , Tousignant Renee N. , Levin Albert M. , Boulware David , Chitale Dhananjay A. , Shaw Brandon M. , Chen Zhihua , Zhang Yonghong , Blakeley Jaishri O. , Acosta Maria T. , Messiaen Ludwine M. , Korf Bruce R. , Tainsky Michael A. 
Published: September 2017

AbstractNF1 mutations predispose to neurofibromatosis type 1 (NF1) and women with NF1 have a moderately elevated risk for breast cancer, especially under age 50. Germline genomic analysis may better define the risk so screening and prevention can be applied to the individuals who benefit the most. Survey conducted in several neurofibromatosis clinics in the United States has demonstrated a 17.2% lifetime risk of breast cancer in women affected with NF1. Cumulated risk to age 50 is estimated to be 9.27%. For genomic profiling, fourteen women with NF1 and a history of breast cancer were recruited and underwent whole exome sequencing (WES), targeted genomic DNA based and RNA‐based analysis of the NF1 gene. Deleterious NF1 pathogenic variants were identified in each woman. Frameshift mutations because of deletion/duplication/complex rearrangement were found in 50% (7/14) of the cases, nonsense mutations in 21% (3/14), in‐frame splice mutations in 21% (3/14), and one case of missense mutation (7%, 1/14). No deleterious mutation was found in the following high/moderate‐penetrance breast cancer genes: ATM, BRCA1, BRCA2, BARD1, BRIP1, CDH1, CHEK2, FANCC, MRE11A, NBN, PALB2, PTEN, RAD50, RAD51C, TP53, and STK11. Twenty‐five rare or common variants in cancer related genes were discovered and may have contributed to the breast cancers in these individuals. Breast cancer predisposition modifiers in women with NF1 may involve a great variety of molecular and cellular functions.
Patient satisfaction with health care services at a national institute of ophthalmology
Authors: Van Huy Nguyen , Dung Nguyen Ngoc , Thang Cao Duc , Hanh Le Thuy 
Published: September 2017

SummaryLittle is known about how patients in developing countries, such as Vietnam, are satisfied with eye care services. The purpose of this study was to assess the satisfaction with health services and its associated factors among patients attending a national institute of ophthalmology in Vietnam. In a cross‐sectional study utilizing quantitative methods, 500 inpatients and their relatives attending a national institute of ophthalmology in Vietnam were approached for data collection. The results indicated that under 50% of the patients were satisfied with eye care services. However, when classified by level of satisfaction, only 6.8% were very satisfied with all domains of care. There was no significant difference in satisfaction by gender and income, while significant differences by department, residence, and education were found. Patients who were from rural areas, were better educated, and used the services of the glaucoma department, were more satisfied with eye care than those from urban areas, were less educated, and used the services of treatment‐on‐demand department. Multivariable regression detected 2 main factors, gender and location, associated with patient satisfaction. Patients who were female and came from rural and remote areas were more likely to be satisfied than patients who were male and living in urban areas. The study suggests that to continue to improve health care quality, it is important to eliminate differences in providing eye care services regardless of whether patients are male or female, and whether they come from a rural or urban area.
Long‐term exposure to bisphenol S damages the visual system and reduces the tracking capability of male zebrafish (Danio rerio)
Authors: Liu Wenmin , Zhang Xiaona , Wei Penghao , Tian Hua , Wang Wei , Ru Shaoguo 
Published: September 2017

AbstractBisphenol S (BPS) is widely detected in aquatic environments and in human bodies. BPS has reproductive and thyroid disrupting effects, but its effect on the visual system remains unknown. In the present study, zebrafish embryos were exposed to BPS at concentrations of 1, 10, 100 and 1000 μg l−1 until 120 days post‐fertilization in a semistatic system, and the effect of BPS on the visual behavior was examined using the optokinetic response and the optomotor response tests in male zebrafish. The retinal histology, mRNA expression of photoreceptor opsin genes (zfrho, zfblue, zfgr1, zfred and zfuv) and apoptosis‐related genes (bax and bcl‐2) were also assessed. Long‐term BPS exposure decreased the tracking capability of male zebrafish, consistent with structural damage to the retina. BPS induced different amounts of vacuoles in the retinal pigment epithelium, and 1000 μg l−1 BPS exposure decreased the length of the inner plexiform layer, ganglion cell layer and retina, and induced an irregular arrangement of photoreceptor cells. The expression levels of the opsin genes (zfred, zfgr1 and zfrho) were significantly elevated, indicating an enhanced spectral sensitivity to red, green and dim light to compensate for the reduction of the optomotor response. Together, the results showed for the first time that long‐term exposure to BPS damaged the structure of male zebrafish retina and reduced their tracking capability.
Three‐dimensional reconstruction of the pharyngeal gland cells in the predatory nematode Pristionchus pacificus
Authors: Riebesell Metta , Sommer Ralf J. 
Published: September 2017

AbstractPristionchus pacificus is a model system in evolutionary biology and for comparison to Caenorhabditis elegans. As a necromenic nematode often found in association with scarab beetles, P. pacificus exhibits omnivorous feeding that is characterized by a mouth‐form dimorphism, an example of phenotypic plasticity. Eurystomatous animals have a dorsal and a sub‐ventral tooth enabling predatory feeding on other nematodes whereas stenostomatous animals have only a dorsal tooth and are microbivorous. Both mouth forms of P. pacificus, like all members of the Diplogastridae family, lack the grinder in the terminal bulb of the pharynx resulting in a fundamentally different organization of several pharynx‐associated structures. Here, we describe the three‐dimensional reconstruction of the pharyngeal gland cells in P. pacificus based on serial transmission electron microscopical analysis of 2527 sections of 50 nm thickness. In comparison to C. elegans, P. pacificus lacks two gland cells (g2) usually associated with grinder function, whereas the three gland cells of g1 (g1D, g1VL, and g1VR) are very prominent. The largest expansion is seen for g1D, which has an anterior process that opens into the buccal cavity through a canal in the dorsal tooth. We provide the morphological description and fine structural analysis of the P. pacificus gland cells, the behavior of the pharynx and preliminary insight into exocytosis of gland cell vesicles in P. pacificus.
Seasonal variations in the profile of main phospholipids in Mytilus galloprovincialis mussels: A study by hydrophilic interaction liquid chromatography–electrospray ionization Fourier transform mass spectrometry
Authors: Facchini Laura , Losito Ilario , Cataldi Tommaso R.I. , Palmisano Francesco 
Published: September 2017

AbstractA systematic characterization of phosphatidylcholines and phosphatidylethanolamines in mussels of sp Mytilus galloprovincialis was performed by high‐efficiency hydrophilic interaction liquid chromatography combined with electrospray ionization and Fourier transform mass spectrometry (FTMS), based on a quadrupole‐Orbitrap hybrid spectrometer. The FTMS/MS experiments under high collisional energy dissociation conditions, complemented by low‐energy collisionally induced dissociation MSn (n = 2,3) experiments, performed in a linear ion trap mass spectrometer, were exploited for structural elucidation purposes. The described approach led to an unprecedented characterization of the mussel phospholipidome, with 185 phosphatidylcholines and 131 phosphatidylethanolamines species recognized, distributed among diacylic, plasmanylic, and plasmenylic forms. This was the starting point for the evaluation of the effects of season (in particular, of sea temperature) on the profile of those phospholipids. To this aim, a set of mussel samples retrieved from commercial sources in different periods of the year was considered. Principal component analysis revealed a clear separation between samples collected in periods characterized by cold, intermediate, or warm sea temperatures, respectively. In particular, an enrichment in phospholipids containing unsaturated side chains was observed in mussels collected from cold seawaters (winter‐early spring), thus confirming the general model previously elaborated to explain the adaptation of marine invertebrates, including some bivalve molluscs, to low temperatures. On the other hand, relevant levels of plasma(e)nylic and acylic phospholipids bearing either saturated or non‐methylene‐interrupted side chains were found in mussels collected in warm seawaters (typical of summer and early autumn, at Italian latitudes). This finding opened interesting perspectives towards the development of strategies able to prevent global warming–related mussel losses in aquacultural plants.