bluuebunny/crossref_metadata_2025_split huggingface dataset:
The principles of Health Technology Assessment in laboratory medicine
Authors: Liguori Giorgio , Belfiore Patrizia , D’Amora Maurizio , Liguori Renato , Plebani Mario 
Published: June 2016

Abstract
The Health Technology Assessment (HTA) is a multi-professional and multidisciplinary evaluation approach designed to assess health technology in the broadest sense of the term, from its instruments to the rearranging of its organizational structures. It is by now an established methodology at national and international levels that involves several medical disciplines thanks to its versatility. Laboratory medicine is one of these disciplines. Such specialization was subjected, in recent years, to deep changes even from an organizational standpoint, in order to meet the health needs of the population, making them as effective and cost-effective as possible. In this regard, HTA was the tool used to assess implications in different areas.
Moral education and moral consumption
Authors: Kalajtzidis Ján 
Published: June 2016

Abstract
The main aim of the presented paper is to suggest a new possible approach in moral education in Slovakia. The starting point for the presented argumentation is the position that moral education (ethics education) in Slovakia is based on insufficient foundations. One of the possible propositions of how to overcome this shortcoming is to supersede prosocial behavior (insufficient base) with value education and promotion of the development of critical and analytical moral thinking. The paper suggests that one of the possible ways how to achieve this goal is by the help of introducing the issue of moral (ethical) consumption as a topic of moral education.
Environmental education in Slovakia in the context of ethics and ethics education
Authors: Blahová Adela Lešková 
Published: June 2016

Abstract
This paper focuses on the current state of environmental education within formal education in Slovakia, emphasising its methodology and weak points. The author aims to identify the place and role of philosophicalethical theory within environmental education, which is an integral part of ethics education. What concrete knowledge, skills and instruments of both ethics and philosophy can (should) a teacher of ethics education put into effect when teaching environmental-educational topics? Before answering this question the place of environmental education within the broader context of moral education will be explored.
Moral education in the Czech territory in the past and the present
Authors: Lesňák Slavomír , Štěrba Radim 
Published: June 2016

Abstract
The following study presents an analysis of the world of moral education in the Czech environment from its recorded beginnings to the current situation in the Czech Republic. The study contains two parts: In the first section, the authors give an account of the history of moral education and different views on it, while in the second section they analyse the current state of moral education. The authors examine the quality of moral education at primary and secondary schools according to the goals of curriculum documents issued by the Ministry of Education, Youth and Sport of the Czech Republic for schools’ ethical education programmes and subjects dealing with character development. Based on their own research, they compare set goals with practice, draw conclusions and give recommendations not only to primary and secondary schools, but also to higher education institutions, particularly in terms of introducing professional ethics tools and intensifying their use. The authors suggest expanding the ethical climate at schools to contain the atmosphere of recognition, biophile culture and technological scepticism.
Moral (or ethical) education in Slovakia and its theoretical basis
Authors: Gluchman Vasil 
Published: June 2016

Abstract
With regard to existing concept of the moral education (ethics) in Slovakia, the questions of ethics and morals are only one of the partial sections. The dominant role is played by psychology based on Roberto Olivar’s concept with emphasis on pro-socialization and on Erickson’s concept of the psychosocial development. From the philosophy basis point of view, only Aristotle, even in reduced form and Spranger’s concept of the life forms are mentioned. Philosophy and ethics are only complements to more psychologically based educational program which is resulting from the problematic division of a social and moral experience into egoistic and prosocial. Egoism is presented in a distorted form and is characterized as the cause of all moral evil. However, there are several different types of understanding of the term egoism in philosophy and ethics as for example psychological and ethical egoism, or self-interest. Ethical egoism or self-interest cannot be identified with selfishness. The main aim of moral education should not be only to form the desired children and youth moral orientation but on the other hand, to form morally self-confident individuals who are able to solve the moral problems, to help the others to solve them as well and to be able to bear moral responsibility for their own deeds.
Annexins in plasma membrane repair
Authors: Boye Theresa Louise , Nylandsted Jesper 
Published: June 2016

Abstract
Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling of cortical actin cytoskeleton and vesicle wound patching. Members of the annexin protein family, which are Ca2+-triggered phospholipid-binding proteins emerge as important components of the plasma membrane repair system. Here, we discuss the mechanisms of plasma membrane repair involving annexins spanning from yeast to human cancer cells.
Capillary electrophoresis-integrated immobilized enzyme reactors
Authors: Liu Xiaoxia , Yang Jiqing , Yang Li 
Published: June 2016

Abstract
Online enzyme assay based on capillary electrophoresis (CE) offers several advantages for the assay, such as low consumption of samples, easy automation of all steps, and less requirement of sample work-up. As a widely used approach for online enzyme assay, CE-integrated immobilized enzyme microreactor (IMER) has been applied in almost all aspects of enzyme assays during the past two decades, including evaluation of the enzymatic activity and kinetics, screening of inhibitor, investigation of enzyme-mediated metabolic pathways, and proteome analysis. In a CE-integrated IMER, enzyme is bound to the capillary surface or a suitable carrier attached to the capillary and substrates/products of the enzymatic reaction are separated and online detected by CE at downstream of the capillary. Enzymatic reactions can be viewed as interaction between the stationary phase (immobilized enzyme) and the mobile phase (substrate(s)/co-enzyme(s) solution), in analogy to the well-known separation technique, capillary electrochromatography. From this point of view, CE-integrated IMERs can be categorized into open tubular capillary IMER, monolithic IMER, and packed capillary IMER. In this review, we have surveyed, analyzed, and discussed advances on fabrication techniques of the three categories of CE-integrated IMERs for online assays involving various enzymes in the past two decades (1992–2015). Some recent studies using microfluidic-based IMERs for enzyme assays have also been reviewed.
Proliferating Myositis: An Inflammatory Lesion often Misdiagnosed as A Malignant Tumor
Authors: Binesh Fariba , Sobhanardekani Mohammad , Zabihi Somayeh , Behniafard Nasim 
Published: June 2016

Abstract
Proliferative myositis (PM) is a rare inflammatory disease. Most commonly, the lesion occurs in the extremities. Regarding its fast growth and bizarre shape of the cellular components this entity commonly misdiagnosed and the patients undergo improper therapeutic approaches. In other words, it is often misdiagnosed as sarcoma. The diagnosis can only be made by the microscopic examination, so biopsy is mandatory. Here the authors report a patient with PM who was initially misdiagnosed as pleomorphic sarcoma of the lower extremity and explain this rare entity.
Proliferative myositis should be taken into account if a fast growing, intramuscular mass occurs in the extremities.
Effects of Spectrum Holdings on Equilibrium in the Wireless Industry
Authors: Lhost Jonathan , Pinto Brijesh , Sibley David 
Published: June 2016

AbstractWe propose a model of Bertrand competition in which consumers choose firms based on prices and qualities. Service quality depends on congestion, which is a function of capacity and output. We first present theoretical properties of the model. Next, we calibrate the model to the wireless industry and use it to evaluate the impacts of changes in spectrum allocation on consumer welfare and profits. Simulations of the model show that when one firm acquires more spectrum, consumer welfare at all firms increases due to congestion externality effects. We find that a transfer of spectrum from one firm to another can either raise or lower consumer welfare at the firms not involved in the transaction, again due to externality effects. Where it is possible to compare the results of our model to the wireless industry, they are consistent with the data. We also explore some possible effects of the upcoming 2016 spectrum auctions.
Fiscality - A Relevant Factor Influencing Regional Development in Romania and the European Union
Authors: Vlad Cristina , Brezeanu Petre 
Published: June 2016

Abstract
The paper aims to examine taxation in the EU in correlation with regional development measures implemented. We started with the EU vision on regional development. If during the 2007-2013 period, were pursued three major objectives (convergence, regional competitiveness and territorial cooperation), in the current 2014-2020 funding period, money is allocated differently between countries that are deemed to be more developed, in transition and less developed. These categories are set according to GDP per capita. Next we exposed the fiscal changes made in the EU in 2010-2011 period and then we corelate them with the strategy for regional development for five member states: Romania, Bulgaria, Czech Republic , Hungary and Poland. We used data reported by Eurostat regarding the evolution of unemployment rate and for the foreign direct investments in 2007-2012 period. We also brought up and changes required by the new Romanian Fiscal Code. According to it, measures such as reduction of income tax for new micro enterprises or extending the VAT reverse charge mechanism in many sectors of activity, are meant to encourage foreign capital inflows and also to increase the level of regional development. As a general conclusion, we found that there is a direct link between fiscal policy and regional development; fiscal measures implemented influence the level of unemployment, economic growth, and competitiveness in the private sector.
Empirical Study on the Creative Accounting Phenomenon
Authors: Cernusca Lucian , David Delia , Nicolaescu Cristina , Gomoi Bogdan Cosmin 
Published: June 2016

Abstract
The present study aims to analyze the accounting professionals’ point of view as opposed to the students and master students’ one, regarding the creative accounting phenomenon existence and manifestation forms. In order to accomplish this objective, there has been used the poll/investigation, as a research method and the questionnaire, as a research instrument. Within the study, there is suggested the testing of more hypotheses that contribute to the clarifying of the aspects wished to be analyzed through the research. These hypotheses’ acceptance or rejection is based on the „chi-square“ (Karl Pearson) statistical test and rank ordering method. Trying to elaborate a global conclusion of the questionnaire, there could be noticed the fact that over 50% from the questioned accounting students are not tempted to use the creative accounting practices and techniques in order to optimize the taxation without breaking the actual law regulations. At the opposite side, more than a half from the questioned accounting professionals would use these practices without breaking the law regulations that lead to the taxation’s optimization. The creative accounting has a negative connotation if the accurate image of the financial position and of the performance is not targeted because it represents the essential factor for elaborating and grounding the accounting policies. However, the positive side of the creative accounting is not excluded, given that one appeals to the „fair“ professional judgment of the accounting professionals and to the good faith of managers.
An Enormous Interstitial Mestizo? The (Im)possibility of Eurasian Identity in Dutch Postcolonial Novels
Authors: Boudewijn Petra R. 
Published: June 2016

Abstract
This article examines the (im)possibility of Eurasian identity in Dutch postcolonial novels by second-generation authors such as Marion Bloem and Adriaan van Dis. As a result of Indonesia’s decolonisation 300.000 Dutch nationals came from the former Dutch East Indies to the Netherlands. Among them was a large group of Eurasians, people of mixed Dutch and Indonesian descent. Many of whom had never set foot on the so-called motherland. Although Eurasians had belonged to the European community in the tropics, they were perceived as immigrants by the Dutch government and were subjected to an aggressive, far-reaching assimilation policy - fearing they would otherwise become a major social problem. Their offspring, the so-called second generation, is often assumed to struggle with their identity while growing up in a postcolonial society that did not tolerate cultural differences at the time. What constitutes a Eurasian identity, and can such identities exist after the enforced assimilation of Eurasians in the Netherlands? How do second-generation authors look upon their Eurasian background and how do they portray these assumed identity struggles in postcolonial literature? The texts in question are discussed in relation to theories of hybridity. It is argued that the widespread notion that Eurasians either fall between two stools or grow into examples of hybrid identity are not foregone conclusions.
Fostering Intercultural Competence of Students during Translation Classes at the John Paul II Catholic University of Lublin
Authors: Waterlot Muriel 
Published: June 2016

Abstract
Intercultural communication has become a scientific discipline which aims at improving communication during intercultural contacts by means of fostering the intercultural awareness and competence of the interlocutors involved within the intercultural communication process.
In view of the intensifying European and international contacts the interest for this competency has grown during the last decades. This can among others be observed within foreign language teaching, but also in the training of translators at university level where new didactical approaches and teaching methods are being developed in order to improve the intercultural of future translators.
This article presents a preliminary collaboration between the John Paul II Catholic University of Lublin and non-profit organization of translators, as well as the theoretical and practical backgrounds of a project which that they envisage to realize in future in order to transform its participants into intercultural competent translators.
Reliable Sampled-Data Control of Fuzzy Markovian Systems with Partly Known Transition Probabilities
Authors: Sakthivel R. , Kaviarasan B. , Kwon O.M. , Rathika M. 
Published: June 2016

Abstract
This article presents a fuzzy dynamic reliable sampled-data control design for nonlinear Markovian jump systems, where the nonlinear plant is represented by a Takagi–Sugeno fuzzy model and the transition probability matrix for Markov process is permitted to be partially known. In addition, a generalised as well as more practical consideration of the real-world actuator fault model which consists of both linear and nonlinear fault terms is proposed to the above-addressed system. Then, based on the construction of an appropriate Lyapunov–Krasovskii functional and the employment of convex combination technique together with free-weighting matrices method, some sufficient conditions that promising the robust stochastic stability of system under consideration and the existence of the proposed controller are derived in terms of linear matrix inequalities, which can be easily solved by any of the available standard numerical softwares. Finally, a numerical example is provided to illustrate the validity of the proposed methodology.
La Evaluación: una estrategia para re-pensar las prácticas institucionales
Authors: Ferreyra Horacio Ademar , Eberle María Jacinta 
Published: June 2016

En este siglo de permanentes cambios y de una marcada saturaciónen la producción y avance de la información es sumamente importante que las instituciones educativas puedan avanzar en la construcción de sus propios procesos de calidad,para poder dar respuesta, de esta manera, a los requerimientos del entorno a partir de la formación deciudadanos que posean las competencias necesarias y que se puedan desempeñar como personasfelices en esta sociedad en la que forman parte.Es por ello que dentro de las instituciones se hace cada vez más indispensable la construcción de unanueva cultura evaluativa que utilice la evaluación como estrategia para mejorar y potenciar el desarrollode todos sus miembros a partir de la re-flexión de sus prácticas cotidianas. Este trabajo sólo puede desarrollarsesi se toma como punto de partida la autonomía institucional y si se comprende que la escuelasólo podrá ejecutar su proyecto de mejora si esta evaluación se realiza a partir de la integración demiradas tanto internas como externas. Sólo de esta manera es posible contribuir con el crecimientode la institución y por ende de un pueblo, de una provincia, de una nación.
Whole Genome Sequencing Identifies a Novel Factor Required for Secretory Granule Maturation inTetrahymena thermophila
Authors: Kontur Cassandra , Kumar Santosh , Lan Xun , Pritchard Jonathan K , Turkewitz Aaron P 
Published: June 2016

AbstractUnbiased genetic approaches have a unique ability to identify novel genes associated with specific biological pathways. Thanks to next generation sequencing, forward genetic strategies can be expanded to a wider range of model organisms. The formation of secretory granules, called mucocysts, in the ciliate Tetrahymena thermophila relies, in part, on ancestral lysosomal sorting machinery, but is also likely to involve novel factors. In prior work, multiple strains with defects in mucocyst biogenesis were generated by nitrosoguanidine mutagenesis, and characterized using genetic and cell biological approaches, but the genetic lesions themselves were unknown. Here, we show that analyzing one such mutant by whole genome sequencing reveals a novel factor in mucocyst formation. Strain UC620 has both morphological and biochemical defects in mucocyst maturation—a process analogous to dense core granule maturation in animals. Illumina sequencing of a pool of UC620 F2 clones identified a missense mutation in a novel gene called MMA1 (Mucocyst maturation). The defects in UC620 were rescued by expression of a wild-type copy of MMA1, and disrupting MMA1 in an otherwise wild-type strain phenocopies UC620. The product of MMA1, characterized as a CFP-tagged copy, encodes a large soluble cytosolic protein. A small fraction of Mma1p-CFP is pelletable, which may reflect association with endosomes. The gene has no identifiable homologs except in other Tetrahymena species, and therefore represents an evolutionarily recent innovation that is required for granule maturation.
Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte
Authors: Florea Simona , Phillips Timothy D , Panaccione Daniel G , Farman Mark L , Schardl Christopher L 
Published: June 2016

AbstractMolecular genetic techniques to precisely eliminate genes in asexual filamentous fungi require the introduction of a marker gene into the target genome. We developed a novel strategy to eliminate genes or gene clusters located in subterminal regions of chromosomes, and then eliminate the marker gene and vector backbone used in the transformation procedure. Because many toxin gene clusters are subterminal, this method is particularly suited to generating nontoxic fungal strains. We tested this technique on Epichloë coenophiala, a seed-transmissible symbiotic fungus (endophyte) of the important forage grass, tall fescue (Lolium arundinaceum). The endophyte is necessary for maximal productivity and sustainability of this grass but can produce ergot alkaloids such as ergovaline, which are toxic to livestock. The genome sequence of E. coenophiala strain e19 revealed two paralogous ergot alkaloid biosynthesis gene clusters, designated EAS1 and EAS2. EAS1 was apparently subterminal, and the lpsB copy in EAS2 had a frame-shift mutation. We designed a vector with a fungal-active hygromycin phosphotransferase gene (hph), an lpsA1 gene fragment for homologous recombination at the telomere-distal end of EAS1, and a telomere repeat array positioned to drive spontaneous loss of hph and other vector sequences, and to stabilize the new chromosome end. We transformed E. coenophiala with this vector, then selected “knockoff” endophyte strains, confirmed by genome sequencing to lack 162 kb of a chromosome end including most of EAS1, and also to lack vector sequences. These ∆EAS1 knockoff strains produced no detectable ergovaline, whereas complementation with functional lpsB restored ergovaline production.
Variation and Evolution in the Glutamine-Rich Repeat Region of Drosophila Argonaute-2
Authors: Palmer William H , Obbard Darren J 
Published: June 2016

Abstract
RNA interference pathways mediate biological processes through Argonaute-family proteins, which bind small RNAs as guides to silence complementary target nucleic acids . In insects and crustaceans Argonaute-2 silences viral nucleic acids, and therefore acts as a primary effector of innate antiviral immunity. Although the function of the major Argonaute-2 domains, which are conserved across most Argonaute-family proteins, are known, many invertebrate Argonaute-2 homologs contain a glutamine-rich repeat (GRR) region of unknown function at the N-terminus . Here we combine long-read amplicon sequencing of Drosophila Genetic Reference Panel (DGRP) lines with publicly available sequence data from many insect species to show that this region evolves extremely rapidly and is hyper-variable within species. We identify distinct GRR haplotype groups in Drosophila melanogaster, and suggest that one of these haplotype groups has recently risen to high frequency in a North American population. Finally, we use published data from genome-wide association studies of viral resistance in D. melanogaster to test whether GRR haplotypes are associated with survival after virus challenge. We find a marginally significant association with survival after challenge with Drosophila C Virus in the DGRP, but we were unable to replicate this finding using lines from the Drosophila Synthetic Population Resource panel.