bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carnivorous Nepenthes pitcher plants combine common developmental processes to make a complex epidermal trapping surface
Authors: Lessware Oona C , Mantell Judith M , Bauer Ulrike 
Published: September 2024

Abstract

Background and Aims
A hierarchical micro-topography of ridges and steps renders the trap rim of carnivorous Nepenthes pitcher plants unusually wettable, and slippery for insects when wet. This complex three-dimensional epidermis structure forms, hidden from plain sight, inside the still-closed developing pitcher bud. Here, we reveal the sequence of epidermal patterning events that shape the trap rim. By linking this sequence to externally visible markers of bud development, we provide a framework for targeting individual stages of surface development in future studies.

Methods
We used cryo-scanning electron microscopy to investigate the detailed morphogenesis and epidermal patterning of the Nepenthes × hookeriana pitcher rim. In addition, we collected morphometric and qualitative data from developing pitcher traps including those sampled for microscopy.

Key Results
We identified three consecutive patterning events. First, strictly oriented cell divisions resulted in radially aligned rows of cells and established a macroscopic ridge-and-groove pattern. Next, conical papillate cells formed, and papillae elongated towards the trap interior, increasingly overlapping adjacent cells and eventually forming continuous microscopic ridges. In between these ridges, the flattened papillae formed acutely angled arched steps. Finally, the cells elongated radially, thereby establishing the convex collar shape of the rim. This general sequence of surface development also showed a spatial progression from the outer to the inner trap rim edge, with several consecutive developmental stages co-occurring at any given time.

Conclusions
We demonstrate that the complex surface micro-topography of the Nepenthes pitcher rim develops by sequentially combining widespread, evolutionarily conserved epidermal patterning processes in a new way. This makes the Nepenthes trap rim an excellent model for studying epidermal patterning mechanisms in leaves.
Single-cell RNA sequencing reveals the important role of Dcaf17 in spermatogenesis of golden hamsters
Authors: Ma Rongzhu , Liang Shuang , Zeng Wentao , Li Jianmin , Lai Yana , Yang Xiaoyu , Diao Feiyang 
Published: September 2024

Abstract
Dcaf17, also known as DDB1- and CUL4-associated factor 17, is a member of the DCAF family and acts as the receptor for the CRL4 ubiquitin E3 ligase complex. Several previous studies have reported that mutations in Dcaf17 cause Woodhouse–Sakati syndrome, which results in oligoasthenoteratozoospermia and male infertility. As a model to explore the role of Dcaf17 in the male reproductive system, we created Dcaf17-deficient male golden hamsters using CRISPR-Cas9 technology; the results of which demonstrate that deletion of Dcaf17 led to abnormal spermatogenesis and infertility. To uncover the underlying molecular mechanisms involved, we conducted single cell Ribonucleic Acid sequencing analysis to evaluate the effect of Dcaf17 deficiency on transcriptional levels in spermatogenic cells during various stages of spermatogenesis. These data emphasize the significant regulatory role played by Dcaf17 in early spermatogenic cells, with many biological processes being affected, including spermatogenesis and protein degradation. Dysregulation of genes associated with these functions ultimately leads to abnormalities. In summary, our findings highlight the critical function of Dcaf17 in spermatogenesis and clarify the specific stage at which Dcaf17 exerts its effects, while simultaneously providing a novel animal model for the study of Dcaf17.
The Impact of Infectious Diseases Scholarly Mentorship on Subsequent Infectious Disease Fellowship Application
Authors: Marcus Joseph E , Yun Heather C , Barsoumian Alice E 
Published: September 2024

Abstract

Introduction
Infectious diseases physicians invest significant time mentoring medical students and internal medicine residents through research projects as well as case reports. Although having an infectious diseases mentor has been shown to be associated with subsequent infectious diseases fellowship application, the impact of specific scholarly activities on future application to infectious diseases fellowship is unknown.

Methods
All research and case reports published or presented from Brooke Army Medical Center between 2014 and 2022 with an infectious diseases senior author and a medical student or internal medicine resident first author were evaluated. The presentations and publications that resulted from each project as well as whether the trainee applied to infectious diseases were recorded.

Results
During the study period, 16 faculty mentored 35 medical student and resident research projects and 26 case reports. Research and case reports were primarily performed by residents (88% and 96%, respectively). Compared to case reports, research projects were more likely to be presented at national meetings (77% vs 32%, P = .0009). Of the 55 projects performed by trainees who completed training, research was associated with greater rates of infectious disease fellowship application as compared to case reports (41% vs 4%, P = .0012).

Conclusions
Internal medicine resident and medical student involvement in research mentored by an infectious disease physician was associated with a greater infectious diseases fellowship application rate as compared to those who were mentored for case reports. Investment in trainee research may be a strategy for recruiting the next generation of infectious diseases physicians.
An uncommon and challenging finding regarding the tricuspid valve: case report, clinical considerations, and practical management
Authors: Sciatti Edoardo , Limonta Raul , D’Isa Salvatore , Duino Vincenzo , Senni Michele 
Published: September 2024

Abstract

Background
The differential diagnosis of tricuspid masses remains challenging.

Case summary
This case involves the incidental detection of a lesion with a non-solid appearance, exhibiting the characteristic ‘finger-in-glove’ and ‘garland-like’ morphology, resembling a blind-ended protrusion of the tricuspid leaflet. This presentation is consistent with a tricuspid valve aneurysm, without significant associated stenosis or regurgitation.

Discussion
Given the lesion’s morphological features, the patient’s asymptomatic status, and the absence of a precipitating event suggestive of an alternative diagnosis, we concluded that the most likely diagnosis is aseptic tricuspid valve aneurysm. Following a multidisciplinary heart team discussion, surgical intervention was deemed unnecessary.
Acute myocarditis and haemoptysis in an adult with human bocavirus monoinfection: a case report
Authors: Tustiu Iulia , Woods Sara , Lee Jennifer , Buckley Orla , Moore David 
Published: September 2024

Abstract

Background
Bocavirus monoinfection-related acute myocarditis is an aetiology that has rarely been described in the literature.

Case summary
A 36-year-old male, with no significant medical history, presented to the emergency department with a 4-day history of dyspnoea, haemoptysis, left-sided chest pain, and high-grade pyrexia. The initial investigations revealed a raised troponin T, raised C-reactive protein, and a normal electrocardiogram. A comprehensive microbiological and virological work-up (testing for 14 viruses and bacteria) detected human bocavirus (HBoV) DNA monoinfection. Cardiac magnetic resonance imaging showed left ventricular ejection fraction of 48%, with subepicardial late gadolinium enhancement. Other imaging modalities (chest X-ray, echocardiography, computed tomography pulmonary angiography, and bronchoscopy) revealed no other causative pathology. The patient was treated with anti-inflammatory medications and left ventricle remodelling therapy. He had a good clinical outcome. Moreover, a collateral history revealed that the patient’s infant had presented with a severe respiratory illness, which was felt to be of viral aetiology, several days prior to the patient’s own onset of symptoms.

Discussion
To our knowledge, this is the fourth case of HBoV-related acute myocarditis in an immunocompetent adult. This case also displays new clinical features for HBoV infection—haemoptysis, high-grade pyrexia, and a potential for vertical transmission from infants.
Update on antithrombotic therapy and body mass: a clinical consensus statement of the European Society of Cardiology Working Group on Cardiovascular Pharmacotherapy and the European Society of Cardiology Working Group on Thrombosis
Authors: Gigante Bruna , Tamargo Juan , Agewall Stefan , Atar Dan , ten Berg Jurrien , Campo Gianluca , Cerbai Elisabetta , Christersson Christina , Dobrev Dobromir , Ferdinandy Péter , Geisler Tobias , Gorog Diana A , Grove Erik L , Kaski Juan Carlos , Rubboli Andrea , Wassmann Sven , Wallen Håkan , Rocca Bianca 
Published: September 2024

Abstract
Obesity and underweight are a growing health problem worldwide and a challenge for clinicians concerning antithrombotic therapy, due to the associated risks of thrombosis and/or bleeding. This clinical consensus statement updates a previous one published in 2018, by reviewing the most recent evidence on antithrombotic drugs based on body size categories according to the World Health Organization classification. The document focuses mostly on individuals at the extremes of body weight, i.e. underweight and moderate-to-morbid obesity, who require antithrombotic drugs, according to current guidelines, for the treatment or prevention of cardiovascular diseases or venous thromboembolism. Managing antithrombotic therapy or thromboprophylaxis in these individuals is challenging, due to profound changes
in body composition, metabolism and organ function, and altered drug pharmacokinetics and pharmacodynamics, as well as weak or no evidence from clinical trials. The document also includes artificial intelligence simulations derived from in silico pharmacokinetic/pharmacodynamic models, which can mimic the pharmacokinetic changes and help identify optimal regimens of antithrombotic drugs for severely underweight or severely obese individuals. Further, bariatric surgery in morbidly obese subjects is frequently performed worldwide. Bariatric surgery causes specific and additional changes in metabolism and gastrointestinal anatomy, depending on the type of the procedure, which can also impact the pharmacokinetics of antithrombotic drugs and their management. Based on existing literature, the document provides consensus statements on optimizing antithrombotic drug management for underweight and all classes of obese patients, while highlighting the current gaps in knowledge in these complex clinical settings, which require personalized medicine and precision pharmacology.
Social mating systems in birds: resource-defense polygamy—but not lekking—is a macroevolutionarily unstable trait
Authors: Marcondes Rafael S , Douvas Nicolette 
Published: September 2024

Abstract
Our understanding of the evolution of social mating systems is largely based on an atemporal ecological framework, whereas macroevolutionary and phylogenetic perspectives looking at the causes of mating systems variation are less developed. Here, we present analyses of the evolution of social mating systems in birds at an unprecedented scale, including 66% of the world’s birds and using trait-dependent speciation and extinction models. We found that lekking (no social bond between the sexes) is very rarely lost, in accordance with the hypothesis that a male shifting to investing in one rather than multiple mates would suffer a severe fitness cost. In contrast, resource-defense polygamous lineages (with a weak, transient socio-sexual bond) frequently revert back to monogamy (strong, durable socio-sexual bond) and have an elevated extinction fraction. We tentatively attribute this to the impossibility of females settling on an optimal parental care strategy under this system. Finally, we found that most gains of lekking have been directly from monogamy rather than through an intermediate stage of resource-defense polygamy.
Conserved signaling modules regulate filamentous growth in fungi: a model for eukaryotic cell differentiation
Authors: Vandermeulen Matthew D , Lorenz Michael C , Cullen Paul J 
Published: September 2024

Abstract
Eukaryotic organisms are composed of different cell types with defined shapes and functions. Specific cell types are produced by the process of cell differentiation, which is regulated by signal transduction pathways. Signaling pathways regulate cell differentiation by sensing cues and controlling the expression of target genes whose products generate cell types with specific attributes. In studying how cells differentiate, fungi have proved valuable models because of their ease of genetic manipulation and striking cell morphologies. Many fungal species undergo filamentous growth—a specialized growth pattern where cells produce elongated tube-like projections. Filamentous growth promotes expansion into new environments, including invasion into plant and animal hosts by fungal pathogens. The same signaling pathways that regulate filamentous growth in fungi also control cell differentiation throughout eukaryotes and include highly conserved mitogen-activated protein kinase (MAPK) pathways, which is the focus of this review. In many fungal species, mucin-type sensors regulate MAPK pathways to control filamentous growth in response to diverse stimuli. Once activated, MAPK pathways reorganize cell polarity, induce changes in cell adhesion, and promote the secretion of degradative enzymes that mediate access to new environments. However, MAPK pathway regulation is complicated because related pathways can share components with each other yet induce unique responses (i.e. signal specificity). In addition, MAPK pathways function in highly integrated networks with other regulatory pathways (i.e. signal integration). Here, we discuss signal specificity and integration in several yeast models (mainly Saccharomyces cerevisiae and Candida albicans) by focusing on the filamentation MAPK pathway. Because of the strong evolutionary ties between species, a deeper understanding of the regulation of filamentous growth in established models and increasingly diverse fungal species can reveal fundamentally new mechanisms underlying eukaryotic cell differentiation.
Navigating Challenges and Opportunities in Community-Based Interventions for Promoting Active Aging: A Systematic Review of the Roles of Technology
Authors: Chang Fangyuan , Wang Gubing , Gu Zhenyu 
Published: September 2024

Abstract

Background and Objectives
Researchers from different fields are increasingly interested in incorporating technology into community-based interventions that promote active aging. Yet, there lacks a comprehensive understanding of technology roles, posing challenges for designers seeking to maximize the intended impacts of such interventions. This systematic review aims to fill the gap by examining existing community-based interventions that integrate digital technologies to promote active aging.

Research Design and Methods
Thirteen studies were screened, with a total of 14 interventions examined, following the PRISMA Guideline.

Results
The challenges in designing and operating community-based interventions, the roles of technology in the interventions, and the caveats of utilizing technology during the process were identified. The review emphasizes the importance of overcoming challenges in community-based interventions and leveraging technology to enhance the intended impacts.

Discussion and Implications
The findings underscore the need to understand technology’s nuanced roles in community-based interventions for active aging. The article provides a starting point for accumulating knowledge and practice in utilizing technology to navigate the challenges and opportunities encountered in such interventions. It also sheds light on a notable gap: the lack of innovative and strategic approaches that harness digital interventions appropriately in community-based interventions. This emphasizes a crucial requirement for guidelines to assist designers, policymakers, and community staff in integrating technology appropriately into community-based interventions or implementing interventions that incorporate digital technologies at the community level.
Simultaneous joint inversion of surface wave and gravity data for revealing 3-D crustal S-wave velocity and density structures: a case study of the NE Tibetan plateau
Authors: Wang Xiang , Guo Lianghui , Bao Xueyang , Chen Yang 
Published: September 2024

SUMMARY
3-D shear wave velocity and density models are important for understanding the structures, material composition, tectonic deformations and dynamical mechanisms of the Earth's crust. Such models are usually based on surface wave tomography and gravity inversion, which provide high resolution in the vertical and horizontal directions, respectively. The joint inversion of surface wave and gravity methods can promote the mutual constraints and complementary advantages of seismic and gravity information, improving the imaging resolution and reducing the uncertainty in the individual methods. However, the traditional joint inversion methods directly construct only the shear wave velocity model, excluding density models. We present a joint inversion method of surface wave and gravity data that simultaneously constructs both crustal shear wave velocity and density models. Unlike the previous studies, we reconfigure the seismic kernels of surface wave tomography to preserve the seismic kernel of density. Moreover, the gravity kernel of density is combined with the seismic kernels to establish an objective function of simultaneous joint inversion. Consequently, the imaging resolution of density structure is improved. Our method is validated on the northeastern Tibetan plateau. The inversion results show that the Dingxi, Jiuzhaigou and Jishishan earthquakes occurred in the high–low-anomaly transition zones of shear wave velocity or density, implying that they were induced by accumulation of strain energy in the upper crust of the northeastern Tibetan plateau when the surrounding harder blocks extruded during the tectonic deformation process.
Shear-wave velocity structure of the Blanco oceanic transform fault zone
Authors: Adimah Nicholas Irabor , Tan Yen Joe , Russell Joshua Berryman 
Published: September 2024

SUMMARY
Oceanic transform faults (OTFs) facilitate hydrothermal circulation which can modify the fault zone materials and affect their rheological evolution. However, the depth extent and variability of fluid infiltration, degree of mineral alteration and their relationship with earthquake behaviour has only been characterized along a few OTFs globally. Here, we use first-overtone Rayleigh waves extracted from seismic ambient noise to estimate the shear-wave velocity structure beneath the Blanco Transform Fault Zone (BTFZ). Compared to the adjoining normal oceanic plates, relatively variable and slow velocities reduced by at least $\sim$0.2–0.4 km s−1 ($\sim$4–8 per cent) are observed from the crust down to $\sim$22 km depth along some segments of the BTFZ. The crustal slow velocities can be explained by enhanced fluid-filled porosity of $\sim$0.4–10.9 per cent caused by intense fracturing associated with abundant seismicity. Slow uppermost mantle velocities are predominantly consistent with $\sim$1.2–37 per cent serpentinization and $\sim$9 per cent hydration, indicating variable and deep fluid infiltration that exceeds 15 km depth. For instance, shear-wave velocities ($\sim$4.3–4.4 km s−1) in the uppermost mantle beneath the Blanco Ridge suggest extensive serpentinization ($\sim$13–25 per cent), which might explain the recently documented earthquake swarms linked with aseismic creep. In comparison, within the vicinity of the ridge-transform intersections at depths $\sim$$\gt $16 km, low velocities ($\sim$4.1–4.2 km s−1) that are consistent with the presence of up to $\sim$1.6 per cent partial melt suggest intratransform magmatism which would contradict the long-held simple conservative strike-slip characterization of OTFs.
Stress regimes in the Himalaya–Karakoram–Tibet, the western part of India–Eurasia collision: stress field implications based on focal mechanism solution data
Authors: Babu Vivek G , Kumar Naresh , Pal Sanjit Kumar 
Published: September 2024

SUMMARY
The stress regime patterns of high-seismically active regions within the western part of the India–Eurasia collision, spanning from 67° E to 83° E and 27° N to 39° N, are elucidated through analysis of 684 Focal Mechanism Solutions from 1962 to 2021. Eighteen seismically active zones used for the stress tensor inversion, are defined based on the spatial extent of the seismicity, the depth distribution of seismic events, focal mechanism studies, and seismotectonics of the region. The defined regimes are: (1) Sulaiman Ranges and Lobe Region, (2) Hindukush, (3) Pamir, (4) Nanga Parbat Syntaxis, (5) Hazara Syntaxis, (6) Kashmir–Zanskar region, (7) Kangra–Chamba, (8) Kinnaur and Kaurik–Chango fault zone (KCFZ), (9) Garhwal, (10) Kumaon, (11) Karakoram fault zone, and (12) Gozha–Ashikule fault zone. Seismicity is reported only in the crust or up to mid-crust in most of the regions, except for the Pamir and Hindukush, where the seismicity can be observed down to 160 and 280 km, respectively. We report a clockwise rotation of the maximum horizontal stress (SHmax) of about 42° and 21° in the Hindukush and Pamir regions, respectively. with increasing focal depths from north west to north. The region where major and strong earthquakes occur indicates pure compressive regimes. Most of the zones support transpressive and transtensional tectonics with a few zones by normal and strike-slip fault regimes. Regions like Nanga Parbat syntaxis, Kinnaur, KCFZ, and Zanskar are exceptions, where extensional and transformational tectonic features dominate. Plate convergence force has less effect on defining the stress regime in the Karakoram fault zone and Gozha–Ashikule regions, which display transtensional and pure extensional regimes, respectively. Underthrusting of the Indian plate through complex tectonics is indicated by dominant compression stresses with evidences of normal, strike-slip, and oblique fault mechanisms.
Schizophrenia risk-associated SNPs affect expression of microRNA 137 host gene: a postmortem study
Authors: Feng Ningping , Mandal Ajeet , Jambhale Ananya , Narnur Pranav , Chen Gang , Akula Nirmala , Kramer Robin , Kolachana Bhaskar , Xu Qing , McMahon Francis J , Lipska Barbara K , Auluck Pavan K , Marenco Stefano 
Published: September 2024

Abstract
Common variants in the MicroRNA 137 host gene MIR137HG and its adjacent gene DPYD have been associated with schizophrenia risk and the latest Psychiatric Genomics Consortium (PGC). Genome-Wide Association Study on schizophrenia has confirmed and extended these findings. To elucidate the association of schizophrenia risk-associated SNPs in this genomic region, we examined the expression of both mature and immature transcripts of the miR-137 host gene (MIR137HG) in the dorsolateral prefrontal cortex (DLPFC) and subgenual anterior cingulate cortex (sgACC) of postmortem brain samples of donors with schizophrenia and psychiatrically-unaffected controls using qPCR and RNA-Seq approaches. No differential expression of miR-137, MIR137HG, or its transcripts was observed. Two schizophrenia risk-associated SNPs identified in the PGC study, rs11165917 (DLPFC: P = 2.0e-16; sgACC: P = 6.4e-10) and rs4274102 (DLPFC: P = 0.036; sgACC: P = 0.002), were associated with expression of the MIR137HG long non-coding RNA transcript MIR137HG-203 (ENST00000602672.2) in individuals of European ancestry. Carriers of the minor (risk) allele of rs11165917 had significantly lower expression of MIR137HG-203 compared with those carrying the major allele. However, we were unable to validate this result by short-read sequencing of RNA extracted from DLPFC or sgACC tissue. This finding suggests that immature transcripts of MIR137HG may contribute to genetic risk for schizophrenia.