bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
Authors: Zhong Juan , Guo Bin , Xie Jing , Deng Shuwen , Fu Na , Lin Shiyu , Li Guo , Lin Yunfeng , Cai Xiaoxiao 
Published: February 2016

AbstractAdipose-derived stem cells (ASCs) and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation capacity. Our previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing outcomes. However, the paracrine action of ASCs on chondrocytes needs to be further elucidated. In this study, we established a co-culture system to achieve cell-to-cell and cell-to-tissue crosstalk and explored the soluble growth factors in both ASCs and chondrocytes supplemented with 1% fetal bovine serum to mimic the physiological microenvironment. In ASCs, we screened for growth factors by semi-quantitative PCR and quantitative real-time PCR and found that the expression of bone morphogenetic protein 2 (BMP-2), vascular endothelial growth factor B (VEGFB), hypoxia inducible factor-1α (HIF-1α), fibroblast growth factor-2 (FGF-2), and transforming growth factor-β1 significantly increased after co-culture in comparison with mono-culture. In chondrocytes, VEGFA was significantly enhanced after co-culture. Unexpectedly, the expression of collagen II and aggrecan was significantly down-regulated in the co-culture group compared with the mono-culture group. Meanwhile, among all the growth factors screened, we found that the BMP family members BMP-2, BMP-4, and BMP-5 were down-regulated and that VEGFB, HIF-1α, FGF-2, and PDGF were significantly decreased after co-culture. These results suggest that crosstalk between ASCs and chondrocytes is a pathway through the regulated growth factors that might have potential in cartilage repair and regeneration and could be useful for tissue engineering.
Evidence for the stability of the West Antarctic Ice Sheet divide for 1.4 million years
Authors: Hein Andrew S. , Woodward John , Marrero Shasta M. , Dunning Stuart A. , Steig Eric J. , Freeman Stewart P. H. T. , Stuart Finlay M. , Winter Kate , Westoby Matthew J. , Sugden David E. 
Published: February 2016

AbstractPast fluctuations of the West Antarctic Ice Sheet (WAIS) are of fundamental interest because of the possibility of WAIS collapse in the future and a consequent rise in global sea level. However, the configuration and stability of the ice sheet during past interglacial periods remains uncertain. Here we present geomorphological evidence and multiple cosmogenic nuclide data from the southern Ellsworth Mountains to suggest that the divide of the WAIS has fluctuated only modestly in location and thickness for at least the last 1.4 million years. Fluctuations during glacial–interglacial cycles appear superimposed on a long-term trajectory of ice-surface lowering relative to the mountains. This implies that as a minimum, a regional ice sheet centred on the Ellsworth-Whitmore uplands may have survived Pleistocene warm periods. If so, it constrains the WAIS contribution to global sea level rise during interglacials to about 3.3 m above present.
Absence of a spin-signature from a single Ho adatom as probed by spin-sensitive tunneling
Authors: Steinbrecher M. , Sonntag A. , Dias M. dos Santos , Bouhassoune M. , Lounis S. , Wiebe J. , Wiesendanger R. , Khajetoorians A. A. 
Published: February 2016

AbstractWhether rare-earth materials can be used as single-atom magnetic memory is an ongoing debate in recent literature. Here we show, by inelastic and spin-resolved scanning tunnelling-based methods, that we observe a strong magnetic signal and excitation from Fe atoms adsorbed on Pt(111), but see no signatures of magnetic excitation or spin-based telegraph noise for Ho atoms. Moreover, we observe that the indirect exchange field produced by a single Ho atom is negligible, as sensed by nearby Fe atoms. We demonstrate, usingab initiomethods, that this stems from a comparatively weak coupling of the Ho 4felectrons with both tunnelling electrons and substrate-derived itinerant electrons, making both magnetic coupling and detection very difficult when compared to 3delements. We discuss these results in the context of ongoing disputes and clarify important controversies.
Homochiral D4-symmetric metal–organic cages from stereogenic Ru(II) metalloligands for effective enantioseparation of atropisomeric molecules
Authors: Wu Kai , Li Kang , Hou Ya-Jun , Pan Mei , Zhang Lu-Yin , Chen Ling , Su Cheng-Yong 
Published: February 2016

AbstractAbsolute chiral environments are rare in regular polyhedral and prismatic architectures, but are achievable from self-assembly of metal–organic cages/containers (MOCs), which endow us with a promising ability to imitate natural organization systems to accomplish stereochemical recognition, catalysis and separation. Here we report a general assembly approach to homochiral MOCs with robust chemical viability suitable for various practical applications. A stepwise process for assembly of enantiopure ΔΔΔΔΔΔΔΔ- and ΛΛΛΛΛΛΛΛ-Pd6(RuL3)8 MOCs is accomplished by pre-resolution of the Δ/Λ-Ru-metalloligand precursors. The obtained Pd–Ru bimetallic MOCs feature in large D4-symmetric chiral space imposed by the predetermined Ru(II)-octahedral stereoconfigurations, which are substitutionally inert, stable, water-soluble and are capable of encapsulating a dozen guests per cage. Chiral resolution tests reveal diverse host–guest stereoselectivity towards different chiral molecules, which demonstrate enantioseparation ability for atropisomeric compounds with C2 symmetry. NMR studies indicate a distinctive resolution process depending on guest exchange dynamics, which is differentiable between host–guest diastereomers.
Stabilization of active matter by flow-vortex lattices and defect ordering
Authors: Doostmohammadi Amin , Adamer Michael F. , Thampi Sumesh P. , Yeomans Julia M. 
Published: February 2016

AbstractActive systems, from bacterial suspensions to cellular monolayers, are continuously driven out of equilibrium by local injection of energy from their constituent elements and exhibit turbulent-like and chaotic patterns. Here we demonstrate both theoretically and through numerical simulations, that the crossover between wet active systems, whose behaviour is dominated by hydrodynamics, and dry active matter where any flow is screened, can be achieved by using friction as a control parameter. Moreover, we discover unexpected vortex ordering at this wet–dry crossover. We show that the self organization of vortices into lattices is accompanied by the spatial ordering of topological defects leading to active crystal-like structures. The emergence of vortex lattices, which leads to the positional ordering of topological defects, suggests potential applications in the design and control of active materials.
Presynaptic partner selection during retinal circuit reassembly varies with timing of neuronal regeneration in vivo
Authors: Yoshimatsu Takeshi , D’Orazi Florence D. , Gamlin Clare R. , Suzuki Sachihiro C. , Suli Arminda , Kimelman David , Raible David W. , Wong Rachel O. 
Published: February 2016

AbstractWhether neurons can restore their original connectivity patterns during circuit repair is unclear. Taking advantage of the regenerative capacity of zebrafish retina, we show here the remarkable specificity by which surviving neurons reassemble their connectivity upon regeneration of their major input. H3 horizontal cells (HCs) normally avoid red and green cones, and prefer ultraviolet over blue cones. Upon ablation of the major (ultraviolet) input, H3 HCs do not immediately increase connectivity with other cone types. Instead, H3 dendrites retract and re-extend to contact new ultraviolet cones. But, if regeneration is delayed or absent, blue-cone synaptogenesis increases and ectopic synapses are made with red and green cones. Thus, cues directing synapse specificity can be maintained following input loss, but only within a limited time period. Further, we postulate that signals from the major input that shape the H3 HC’s wiring pattern during development persist to restrict miswiring after damage.
Emerging roles of ARHGAP33 in intracellular trafficking of TrkB and pathophysiology of neuropsychiatric disorders
Authors: Nakazawa Takanobu , Hashimoto Ryota , Sakoori Kazuto , Sugaya Yuki , Tanimura Asami , Hashimotodani Yuki , Ohi Kazutaka , Yamamori Hidenaga , Yasuda Yuka , Umeda-Yano Satomi , Kiyama Yuji , Konno Kohtarou , Inoue Takeshi , Yokoyama Kazumasa , Inoue Takafumi , Numata Shusuke , Ohnuma Tohru , Iwata Nakao , Ozaki Norio , Hashimoto Hitoshi , Watanabe Masahiko , Manabe Toshiya , Yamamoto Tadashi , Takeda Masatoshi , Kano Masanobu 
Published: February 2016

AbstractIntracellular trafficking of receptor proteins is essential for neurons to detect various extracellular factors during the formation and refinement of neural circuits. However, the precise mechanisms underlying the trafficking of neurotrophin receptors to synapses remain elusive. Here, we demonstrate that a brain-enriched sorting nexin, ARHGAP33, is a new type of regulator for the intracellular trafficking of TrkB, a high-affinity receptor for brain-derived neurotrophic factor. ARHGAP33 knockout (KO) mice exhibit reduced expression of synaptic TrkB, impaired spine development and neuropsychiatric disorder-related behavioural abnormalities. These deficits are rescued by specific pharmacological enhancement of TrkB signalling in ARHGAP33 KO mice. Mechanistically, ARHGAP33 interacts with SORT1 to cooperatively regulate TrkB trafficking. Human ARHGAP33 is associated with brain phenotypes and reduced SORT1 expression is found in patients with schizophrenia. We propose that ARHGAP33/SORT1-mediated TrkB trafficking is essential for synapse development and that the dysfunction of this mechanism may be a new molecular pathology of neuropsychiatric disorders.
Highly robust crystalsome via directed polymer crystallization at curved liquid/liquid interface
Authors: Wang Wenda , Qi Hao , Zhou Tian , Mei Shan , Han Lin , Higuchi Takeshi , Jinnai Hiroshi , Li Christopher Y. 
Published: February 2016

AbstractLipids and amphiphilic block copolymers spontaneously self-assemble in water to form a plethora of micelles and vesicles. They are typically fluidic in nature and often mechanically weak for applications such as drug delivery and gene therapeutics. Mechanical properties of polymeric materials could be improved by forming crystalline structures. However, most of the self-assembled micelles and vesicles have curved surfaces and precisely tuning crystallization within a nanoscale curved space is challenging, as the curved geometry is incommensurate with crystals having three-dimensional translational symmetry. Herein, we report using a miniemulsion crystallization method to grow nanosized, polymer single-crystal-like capsules. We coin the name crystalsome to describe this unique structure, because they are formed by polymer lamellar crystals and their structure mimics liposomes and polymersomes. Using poly(L-lactic acid) (PLLA) as the model polymer, we show that curved water/p-xylene interface formed by the miniemulsion process can guide the growth of PLLA single crystals. Crystalsomes with the size ranging from ∼148 nm to over 1 μm have been formed. Atomic force microscopy measurement demonstrate a two to three orders of magnitude increase in bending modulus compared with conventional polymersomes. We envisage that this novel structure could shed light on investigating spherical crystallography and drug delivery.
High-efficiency electrochemical thermal energy harvester using carbon nanotube aerogel sheet electrodes
Authors: Im Hyeongwook , Kim Taewoo , Song Hyelynn , Choi Jongho , Park Jae Sung , Ovalle-Robles Raquel , Yang Hee Doo , Kihm Kenneth D. , Baughman Ray H. , Lee Hong H. , Kang Tae June , Kim Yong Hyup 
Published: February 2016

AbstractConversion of low-grade waste heat into electricity is an important energy harvesting strategy. However, abundant heat from these low-grade thermal streams cannot be harvested readily because of the absence of efficient, inexpensive devices that can convert the waste heat into electricity. Here we fabricate carbon nanotube aerogel-based thermo-electrochemical cells, which are potentially low-cost and relatively high-efficiency materials for this application. When normalized to the cell cross-sectional area, a maximum power output of 6.6 W m−2 is obtained for a 51 °C inter-electrode temperature difference, with a Carnot-relative efficiency of 3.95%. The importance of electrode purity, engineered porosity and catalytic surfaces in enhancing the thermocell performance is demonstrated.
Major bacterial lineages are essentially devoid of CRISPR-Cas viral defence systems
Authors: Burstein David , Sun Christine L. , Brown Christopher T. , Sharon Itai , Anantharaman Karthik , Probst Alexander J. , Thomas Brian C. , Banfield Jillian F. 
Published: February 2016

AbstractCurrent understanding of microorganism–virus interactions, which shape the evolution and functioning of Earth’s ecosystems, is based primarily on cultivated organisms. Here we investigate thousands of viral and microbial genomes recovered using a cultivation-independent approach to study the frequency, variety and taxonomic distribution of viral defence mechanisms. CRISPR-Cas systems that confer microorganisms with immunity to viruses are present in only 10% of 1,724 sampled microorganisms, compared with previous reports of 40% occurrence in bacteria and 81% in archaea. We attribute this large difference to the lack of CRISPR-Cas systems across major bacterial lineages that have no cultivated representatives. We correlate absence of CRISPR-Cas with lack of nucleotide biosynthesis capacity and a symbiotic lifestyle. Restriction systems are well represented in these lineages and might provide both non-specific viral defence and access to nucleotides.
Rapid intensification and the bimodal distribution of tropical cyclone intensity
Authors: Lee Chia-Ying , Tippett Michael K. , Sobel Adam H. , Camargo Suzana J. 
Published: February 2016

AbstractThe severity of a tropical cyclone (TC) is often summarized by its lifetime maximum intensity (LMI), and the climatological LMI distribution is a fundamental feature of the climate system. The distinctive bimodality of the LMI distribution means that major storms (LMI >96 kt) are not very rare compared with less intense storms. Rapid intensification (RI) is the dramatic strengthening of a TC in a short time, and is notoriously difficult to forecast or simulate. Here we show that the bimodality of the LMI distribution reflects two types of storms: those that undergo RI during their lifetime (RI storms) and those that do not (non-RI storms). The vast majority (79%) of major storms are RI storms. Few non-RI storms (6%) become major storms. While the importance of RI has been recognized in weather forecasting, our results demonstrate that RI also plays a crucial role in the TC climatology.
Downregulation of TLX induces TET3 expression and inhibits glioblastoma stem cell self-renewal and tumorigenesis
Authors: Cui Qi , Yang Su , Ye Peng , Tian E. , Sun Guoqiang , Zhou Jiehua , Sun Guihua , Liu Xiaoxuan , Chen Chao , Murai Kiyohito , Zhao Chunnian , Azizian Krist T. , Yang Lu , Warden Charles , Wu Xiwei , D'Apuzzo Massimo , Brown Christine , Badie Behnam , Peng Ling , Riggs Arthur D. , Rossi John J. , Shi Yanhong 
Published: February 2016

AbstractGlioblastomas have been proposed to be maintained by highly tumorigenic glioblastoma stem cells (GSCs) that are resistant to current therapy. Therefore, targeting GSCs is critical for developing effective therapies for glioblastoma. In this study, we identify the regulatory cascade of the nuclear receptor TLX and the DNA hydroxylase Ten eleven translocation 3 (TET3) as a target for human GSCs. We show that knockdown of TLX expression inhibits human GSC tumorigenicity in mice. Treatment of human GSC-grafted mice with viral vector-delivered TLX shRNA or nanovector-delivered TLX siRNA inhibits tumour development and prolongs survival. Moreover, we identify TET3 as a potent tumour suppressor downstream of TLX to regulate the growth and self-renewal in GSCs. This study identifies the TLX-TET3 axis as a potential therapeutic target for glioblastoma.
Global Warming Attenuates the Tropical Atlantic-Pacific Teleconnection
Authors: Jia Fan , Wu Lixin , Gan Bolan , Cai Wenju 
Published: February 2016

AbstractChanges in global sea surface temperature (SST) since the end of last century display a pattern of widespread warming intercepted by cooling in the eastern equatorial Pacific and western coasts of the American continent. Studies have suggested that the cooling in the eastern equatorial Pacific may be partly induced by warming in the North Atlantic. However, it remains unknown how stable this inter-tropical teleconnection will be under global warming. Here we show that the inter-tropical teleconnection from the tropical Atlantic to Pacific weakens substantially as the CO2 concentration increases. This reduced impact is related to the El Niño-like warming of the tropical Pacific mean state, which leads to limited seasonal migration of the Pacific inter-tropical convergence zone (ITCZ) and weakened ocean heat transport. A fast decay of the tropical Atlantic SST anomalies in a warmer climate also contributes to the weakened teleconnection. Our study suggests that as greenhouse warming continues, the trend in the tropical Pacific as well as the development of ENSO will be less frequently interrupted by the Atlantic because of this attenuation. The weakened teleconnection is also supported by CMIP5 models, although only a few of these models can capture this inter-tropical teleconnection.
Control of electronic transport in graphene by electromagnetic dressing
Authors: Kristinsson K. , Kibis O. V. , Morina S. , Shelykh I. A. 
Published: February 2016

AbstractWe demonstrated theoretically that the renormalization of the electron energy spectrum near the Dirac point of graphene by a strong high-frequency electromagnetic field (dressing field) drastically depends on polarization of the field. Namely, linear polarization results in an anisotropic gapless energy spectrum, whereas circular polarization leads to an isotropic gapped one. As a consequence, the stationary (dc) electronic transport in graphene strongly depends on parameters of the dressing field: A circularly polarized field monotonically decreases the isotropic conductivity of graphene, whereas a linearly polarized one results in both giant anisotropy of conductivity (which can reach thousands of percents) and the oscillating behavior of the conductivity as a function of the field intensity. Since the predicted phenomena can be observed in a graphene layer irradiated by a monochromatic electromagnetic wave, the elaborated theory opens a substantially new way to control electronic properties of graphene with light.
Reconfigurable logic via gate controlled domain wall trajectory in magnetic network structure
Authors: Murapaka C. , Sethi P. , Goolaup S. , Lew W. S. 
Published: February 2016

AbstractAn all-magnetic logic scheme has the advantages of being non-volatile and energy efficient over the conventional transistor based logic devices. In this work, we present a reconfigurable magnetic logic device which is capable of performing all basic logic operations in a single device. The device exploits the deterministic trajectory of domain wall (DW) in ferromagnetic asymmetric branch structure for obtaining different output combinations. The programmability of the device is achieved by using a current-controlled magnetic gate, which generates a local Oersted field. The field generated at the magnetic gate influences the trajectory of the DW within the structure by exploiting its inherent transverse charge distribution. DW transformation from vortex to transverse configuration close to the output branch plays a pivotal role in governing the DW chirality and hence the output. By simply switching the current direction through the magnetic gate, two universal logic gate functionalities can be obtained in this device. Using magnetic force microscopy imaging and magnetoresistance measurements, all basic logic functionalities are demonstrated.
Possible shift in the ENSO-Indian monsoon rainfall relationship under future global warming
Authors: Azad Sarita , Rajeevan M. 
Published: February 2016

AbstractEI Nino-Southern Oscillation (ENSO) and Indian monsoon rainfall are known to have an inverse relationship, which we have observed in the rainfall spectrum exhibiting a spectral dip in 3–5 y period band. It is well documented that El Nino events are known to be associated with deficit rainfall. Our analysis reveals that this spectral dip (3–5 y) is likely to shift to shorter periods (2.5–3 y) in future, suggesting a possible shift in the relationship between ENSO and monsoon rainfall. Spectral analysis of future climate projections by 20 Coupled Model Intercomparison project 5 (CMIP5) models are employed in order to corroborate our findings. Change in spectral dip speculates early occurrence of drought events in future due to multiple factors of global warming.
Semantic and emotional content of imagined representations in human occipitotemporal cortex
Authors: Mitchell Daniel J , Cusack Rhodri 
Published: February 2016

AbstractMental imagery is a critical cognitive function, clinically important, but poorly understood. When visual objects are perceived, many of their sensory, semantic and emotional properties are represented in occipitotemporal cortex. Visual imagery has been found to activate some of the same brain regions, but it was not known what properties are re-created in these regions during imagery. We therefore examined the representation during imagery for two stimuli in depth, by comparing the pattern of fMRI response to the patterns evoked by the perception of 200 diverse objects chosen to de-correlate their properties. Real-time, adaptive stimulus selection allowed efficient sampling of this broad stimulus space. Our experiments show that occipitotemporal cortex, which encoded sensory, semantic and emotional properties during perception, can robustly represent semantic and emotional properties during imagery, but that these representations depend on the object being imagined and on individual differences in style and reported vividness of imagery.
Predicting the Mutating Distribution at Antigenic Sites of the Influenza Virus
Authors: Xu Hongyang , Yang Yiyan , Wang Shuning , Zhu Ruixin , Qiu Tianyi , Qiu Jingxuan , Zhang Qingchen , Jin Li , He Yungang , Tang Kailin , Cao Zhiwei 
Published: February 2016

AbstractMutations of the influenza virus lead to antigenic changes that cause recurrent epidemics and vaccine resistance. Preventive measures would benefit greatly from the ability to predict the potential distribution of new antigenic sites in future strains. By leveraging the extensive historical records of HA sequences for 90 years, we designed a computational model to simulate the dynamic evolution of antigenic sites in A/H1N1. With templates of antigenic sequences, the model can effectively predict the potential distribution of future antigenic mutants. Validation on 10932 HA sequences from the last 16 years showing that the mutated antigenic sites of over 94% of reported strains fell in our predicted profile. Meanwhile, our model can successfully capture 96% of antigenic sites in those dominant epitopes. Similar results are observed on the complete set of H3N2 historical data, supporting the general applicability of our model to multiple sub-types of influenza. Our results suggest that the mutational profile of future antigenic sites can be predicted based on historical evolutionary traces despite the widespread, random mutations in influenza. Coupled with closely monitored sequence data from influenza surveillance networks, our method can help to forecast changes in viral antigenicity for seasonal flu and inform public health interventions.