bluuebunny/crossref_metadata_2025_split huggingface dataset:
Central and peripheral clocks are coupled by a neuropeptide pathway in Drosophila
Authors: Selcho Mareike , Millán Carola , Palacios-Muñoz Angelina , Ruf Franziska , Ubillo Lilian , Chen Jiangtian , Bergmann Gregor , Ito Chihiro , Silva Valeria , Wegener Christian , Ewer John 
Published: May 2017

AbstractAnimal circadian clocks consist of central and peripheral pacemakers, which are coordinated to produce daily rhythms in physiology and behaviour. Despite its importance for optimal performance and health, the mechanism of clock coordination is poorly understood. Here we dissect the pathway through which the circadian clock of Drosophila imposes daily rhythmicity to the pattern of adult emergence. Rhythmicity depends on the coupling between the brain clock and a peripheral clock in the prothoracic gland (PG), which produces the steroid hormone, ecdysone. Time information from the central clock is transmitted via the neuropeptide, sNPF, to non-clock neurons that produce the neuropeptide, PTTH. These secretory neurons then forward time information to the PG clock. We also show that the central clock exerts a dominant role on the peripheral clock. This use of two coupled clocks could serve as a paradigm to understand how daily steroid hormone rhythms are generated in animals.
Sea-level records from the U.S. mid-Atlantic constrain Laurentide Ice Sheet extent during Marine Isotope Stage 3
Authors: Pico T , Creveling J. R. , Mitrovica J. X. 
Published: May 2017

AbstractThe U.S. mid-Atlantic sea-level record is sensitive to the history of the Laurentide Ice Sheet as the coastline lies along the ice sheet’s peripheral bulge. However, paleo sea-level markers on the present-day shoreline of Virginia and North Carolina dated to Marine Isotope Stage (MIS) 3, from 50 to 35 ka, are surprisingly high for this glacial interval, and remain unexplained by previous models of ice age adjustment or other local (for example, tectonic) effects. Here, we reconcile this sea-level record using a revised model of glacial isostatic adjustment characterized by a peak global mean sea level during MIS 3 of approximately −40 m, and far less ice volume within the eastern sector of the Laurentide Ice Sheet than traditional reconstructions for this interval. We conclude that the Laurentide Ice Sheet experienced a phase of very rapid growth in the 15 kyr leading into the Last Glacial Maximum, thus highlighting the potential of mid-field sea-level records to constrain areal extent of ice cover during glacial intervals with sparse geological observables.
Solitonic conduction of electrotonic signals in neuronal branchlets with polarized microstructure
Authors: Poznanski R. R. , Cacha L. A. , Al-Wesabi Y. M. S. , Ali J. , Bahadoran M. , Yupapin P. P. , Yunus J. 
Published: May 2017

AbstractA model of solitonic conduction in neuronal branchlets with microstructure is presented. The application of cable theory to neurons with microstructure results in a nonlinear cable equation that is solved using a direct method to obtain analytical approximations of traveling wave solutions. It is shown that a linear superposition of two oppositely directed traveling waves demonstrate solitonic interaction: colliding waves can penetrate through each other, and continue fully intact as the exact pulses that entered the collision. These findings indicate that microstructure when polarized can sustain solitary waves that propagate at a constant velocity without attenuation or distortion in the absence of synaptic transmission. Solitonic conduction in a neuronal branchlet arising from polarizability of its microstructure is a novel signaling mode of electrotonic signals in thin processes (<0.5 μm diameter).
Cathepsin S inhibition combines control of systemic and peripheral pathomechanisms of autoimmune tissue injury
Authors: Tato Maia , Kumar Santhosh V. , Liu Yajuan , Mulay Shrikant R. , Moll Solange , Popper Bastian , Eberhard Jonathan N. , Thomasova Dana , Rufer Arne Christian , Gruner Sabine , Haap Wolfgang , Hartmann Guido , Anders Hans-Joachim 
Published: May 2017

AbstractCathepsin(Cat)-S processing of the invariant chain-MHC-II complex inside antigen presenting cells is a central pathomechanism of autoimmune-diseases. Additionally, Cat-S is released by activated-myeloid cells and was recently described to activate protease-activated-receptor-(PAR)-2 in extracellular compartments. We hypothesized that Cat-S blockade targets both mechanisms and elicits synergistic therapeutic effects on autoimmune tissue injury. MRL-(Fas)lpr mice with spontaneous autoimmune tissue injury were treated with different doses of Cat-S inhibitor RO5459072, mycophenolate mofetil or vehicle. Further, female MRL-(Fas)lpr mice were injected with recombinant Cat-S with/without concomitant Cat-S or PAR-2 blockade. Cat-S blockade dose-dependently reversed aberrant systemic autoimmunity, e.g. plasma cytokines, activation of myeloid cells and hypergammaglobulinemia. Especially IgG autoantibody production was suppressed. Of note (MHC-II-independent) IgM were unaffected by Cat-S blockade while they were suppressed by MMF. Cat-S blockade dose-dependently suppressed immune-complex glomerulonephritis together with a profound and early effect on proteinuria, which was not shared by MMF. In fact, intravenous Cat-S injection induced severe glomerular endothelial injury and albuminuria, which was entirely prevented by Cat-S or PAR-2 blockade.In-vitrostudies confirm that Cat-S induces endothelial activation and injury via PAR-2. Therapeutic Cat-S blockade suppresses systemic and peripheral pathomechanisms of autoimmune tissue injury, hence, Cat-S is a promising therapeutic target in lupus nephritis.
Superlattice growth and rearrangement during evaporation-induced nanoparticle self-assembly
Authors: Josten Elisabeth , Wetterskog Erik , Glavic Artur , Boesecke Peter , Feoktystov Artem , Brauweiler-Reuters Elke , Rücker Ulrich , Salazar-Alvarez German , Brückel Thomas , Bergström Lennart 
Published: May 2017

AbstractUnderstanding the assembly of nanoparticles into superlattices with well-defined morphology and structure is technologically important but challenging as it requires novel combinations of in-situ methods with suitable spatial and temporal resolution. In this study, we have followed evaporation-induced assembly during drop casting of superparamagnetic, oleate-capped γ-Fe2O3 nanospheres dispersed in toluene in real time with Grazing Incidence Small Angle X-ray Scattering (GISAXS) in combination with droplet height measurements and direct observation of the dispersion. The scattering data was evaluated with a novel method that yielded time-dependent information of the relative ratio of ordered (coherent) and disordered particles (incoherent scattering intensities), superlattice tilt angles, lattice constants, and lattice constant distributions. We find that the onset of superlattice growth in the drying drop is associated with the movement of a drying front across the surface of the droplet. We couple the rapid formation of large, highly ordered superlattices to the capillary-induced fluid flow. Further evaporation of interstitital solvent results in a slow contraction of the superlattice. The distribution of lattice parameters and tilt angles was significantly larger for superlattices prepared by fast evaporation compared to slow evaporation of the solvent.
Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Authors: Crasson Oscar , Courtade Gaston , Léonard Raphaël R. , Aachmann Finn Lillelund , Legrand François , Parente Raffaella , Baurain Denis , Galleni Moreno , Sørlie Morten , Vandevenne Marylène 
Published: May 2017

AbstractChitin is an important structural component of numerous fungal pathogens and parasitic nematodes. The human macrophage chitotriosidase (HCHT) is a chitinase that hydrolyses glycosidic bonds between the N-acetyl-D-glucosamine units of this biopolymer. HCHT belongs to the Glycoside Hydrolase (GH) superfamily and contains a well-characterized catalytic domain appended to a chitin-binding domain (ChBDCHIT1). Although its precise biological function remains unclear, HCHT has been described to be involved in innate immunity. In this study, the molecular basis for interaction with insoluble chitin as well as with soluble chito-oligosaccharides has been determined. The results suggest a new mechanism as a common binding mode for many Carbohydrate Binding Modules (CBMs). Furthermore, using a phylogenetic approach, we have analysed the modularity of HCHT and investigated the evolutionary paths of its catalytic and chitin binding domains. The phylogenetic analyses indicate that the ChBDCHIT1 domain dictates the biological function of HCHT and not its appended catalytic domain. This observation may also be a general feature of GHs. Altogether, our data have led us to postulate and discuss that HCHT acts as an immune catalyser.
Normalization of adiponectin concentrations by leptin replacement in ob/ob mice is accompanied by reductions in systemic oxidative stress and inflammation
Authors: Frühbeck Gema , Catalán Victoria , Rodríguez Amaia , Ramírez Beatriz , Becerril Sara , Portincasa Piero , Gómez-Ambrosi Javier 
Published: May 2017

AbstractThe circulating concentrations of adiponectin, an antidiabetic adipokine, have been shown to be reduced in obesity, in relation to an increase in inflammation. The aim of the present work was to assess the effect of leptin replacement on adiponectin levels and expression as well as on markers of oxidative stress and inflammation in leptin-deficient ob/ob mice. Twelve-week-old male mice (n = 7–10 per group) were treated with either saline (wild type and ob/ob mice) or leptin (ob/ob mice) for 18 days. A third group of ob/ob mice was treated with saline and pair-fed to the amount of food consumed by the leptin-treated group. Leptin replacement restored values of adiponectin (P < 0.001), reduced circulating 8-isoprostane and serum amyloid A (SAA) levels (P < 0.05 for both), and significantly downregulated the increased gene expression of osteopontin (Spp1, P < 0.05), Saa3 (P < 0.05), Cd68 (P < 0.01), Il6 (P < 0.01) and NADPH oxidase (Nox1 and Nox2, P < 0.01) in the perirenal WAT and Spp1 (P < 0.05) in the liver of ob/ob mice. In cultured adipocytes from ob/ob mice, leptin increased (P < 0.05) the mRNA expression and secretion of adiponectin. We concluded that circulating concentrations of adiponectin are positively regulated by leptin and ameliorate obesity-associated oxidative stress and inflammation in mice.
Population genetics analysis of the Nujiang catfish Creteuchiloglanis macropterus through a genome-wide single nucleotide polymorphisms resource generated by RAD-seq
Authors: Kang Jingliang , Ma Xiuhui , He Shunping 
Published: May 2017

AbstractAdvances in genome scanning using high-throughput sequencing technologies has led to a revolution in studies of non-model organisms. The glyptosternoid fish Creteuchiloglanis macropterus, is widely distributed in the main stem and tributaries of the Nujiang River basin. Here, we analyzed IIB restriction-site-associated DNA (2b-RAD) sequences and mitochondrial DNA sequences, to assess the genomic signature of adaptation by detecting and estimating the degree of genetic differentiation among ten Creteuchiloglanis macropterus populations from the Nujiang River. The analyses revealed significant population differentiation among the up-tributaries, main stem, mid-tributary and low-tributary. Annotation of contigs containing outlier SNPs revealed that the candidate genes showed significant enrichment in several important biological process terms between up-tributaries and low-tributary, and exhibited prominent enrichment in the term macromolecular metabolic process between all tributaries and the main stem. Population dynamics analyses indicated that the Late Pleistocene glaciations strongly influenced the demographic history of C. macropterus. Our results provide strong evidence for the utility of RAD-seq in population genetics studies, and our generated SNP resource should provide a valuable tool for population genomics studies of C. macropterus in the future.
Stiff, light, strong and ductile: nano-structured High Modulus Steel
Authors: Springer H. , Baron C. , Szczepaniak A. , Uhlenwinkel V. , Raabe D. 
Published: May 2017

AbstractStructural material development for lightweight applications aims at improving the key parameters strength, stiffness and ductility at low density, but these properties are typically mutually exclusive. Here we present how we overcome this trade-off with a new class of nano-structured steel – TiB2 composites synthesised in-situ via bulk metallurgical spray-forming. Owing to the nano-sized dispersion of the TiB2 particles of extreme stiffness and low density – obtained by the in-situ formation with rapid solidification kinetics – the new material has the mechanical performance of advanced high strength steels, and a 25% higher stiffness/density ratio than any of the currently used high strength steels, aluminium, magnesium and titanium alloys. This renders this High Modulus Steel the first density-reduced, high stiffness, high strength and yet ductile material which can be produced on an industrial scale. Also ideally suited for 3D printing technology, this material addresses all key requirements for high performance and cost effective lightweight design.
Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
Authors: Sonah Humira , Deshmukh Rupesh K. , Labbé Caroline , Bélanger Richard R. 
Published: May 2017

AbstractAquaporins (AQPs) are of vital importance in the cellular transport system of all living organisms. In this study, genome-wide identification, distribution, and characterization of AQPs were determined in Arabidopsis lyrata, Capsella grandiflora, C. rubella, Eutrema salsugineum, Brassica rapa, B. oleracea, and B. napus (canola). Classification and phylogeny of AQPs revealed the loss of XIPs and NIP-IIIs in all species. Characterization of distinctive AQP features showed a high level of conservation in spacing between NPA-domains, and selectivity filters. Interestingly, TIP3s were found to be highly expressed in developing seeds, suggesting their role in seed desiccation. Analysis of available RNA-seq data obtained under biotic and abiotic stresses led to the identification of AQPs involved in stress tolerance mechanisms in canola. In addition, analysis of the effect of ploidy level, and resulting gene dose effect performed with the different combinations of Brassica A and C genomes revealed that more than 70% of AQPs expression were dose-independent, thereby supporting their role in stress alleviation. This first in-depth characterization of Brassicaceae AQPs highlights transport mechanisms and related physiological processes that could be exploited in breeding programs of stress-tolerant cultivars.
NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells
Authors: Yoon Jung Hwan , Kim Olga , Nam Suk Woo , Lee Jung Young , Park Won Sang 
Published: May 2017

AbstractNKX6.3 plays an important role in gastric epithelial differentiation and also acts as a gastric tumor suppressor. The specific aim of this study was to determine whether NKX6.3 contributes to gastric mucosal barrier function by regulating reactive oxygen species (ROS) production. NKX6.3 reduced ROS production and regulated expression of anti-oxidant genes, including Hace1. In addition, NKX6.3 reduced DNMT1 expression and activity by down-regulating NF-kB family gene transcription. Silencing of Hace1 recovered ROS production, whereas knock-down of DNMT1 and NF-kB reduced ROS production and induced Hace1 expression by hypomethylating its promoter region. In addition, NKX6.3 inhibited CagA effects on cell growth, ROS production, and NF-kB and DNMT1 activity. In gastric mucosae and cancers, NKX6.3 and Hace1 expression was significantly reduced. The NKX6.3 expression was positively correlated with Hace1 and Nrf2 genes, but negatively correlated with DNMT1. Hypermethylation of Hace1 gene was observed only in gastric mucosae with H. pylori, atrophy and intestinal metaplasia. Thus, these results suggest that NKX6.3 inhibits ROS production by inducing the expression of Hace1 via down-regulating NF-kB and DNMT1 activity in gastric epithelial cells.
Switching of spins and entanglement in surface-supported antiferromagnetic chains
Authors: Sivkov Ilia N. , Bazhanov Dmitry I. , Stepanyuk Valeri S. 
Published: May 2017

AbstractPrevious experimental studies discovered universal growth of chains and nanowires of various chemical elements on a corrugated molecular network of Cu3N on the Cu(110). Herein, performing combined ab initio and quantum Hamiltonian studies we demonstrate that such chains can be used for a fast spin switching and entanglement generation by locally applied magnetic pulses. As an example, we show that in antiferromagnetic Co chains a strong entanglement between ends of chains occurs during spin switching. A novel parity effect in spin dynamics is reported. Even-numbered chains are found to exhibit significantly faster spin switching than odd-numbered counterparts. Moreover, at certain parameters of the system the dimerization effect in the spin dynamics of the chains was found. Our studies give a clear evidence that tailoring spin dynamics and entanglement can be achieved by magnetic fields and by tuning exchange interactions in supported chains.
Complex beam shaping based on an equivalent q-plate system and analysis of its properties using digital holography polarization imaging
Authors: Yang Ching-Han , Fuh Andy Ying-Guey 
Published: May 2017

AbstractIn this study, we generate various complex beams carrying angular momentum (AM) by using a programmable beam shaping system to mimic typical q-plates. When a circularly polarized wave is incident onto the system, the emerging beam reverses its spin handedness and obtains a spatial phase factor. This phase factor can be engineered by designing a computer-generated hologram (CGH) and applying it to a spatial light modulator (SLM) to produce a beam with controllable spatially distributed orbital angular momentum (OAM) density. To determine the properties of the generated fields, we combine digital holography (DH) with the beam shaping system to yield visualizations of the beam intensity, phase, and AM distributions over the transverse plane at different propagation distances. Comparisons of the theoretically and experimentally obtained results show good qualitative agreement. This study advances our understanding and interpretation of AM characteristics produced by a programmable q-plate-like system.
Earlier flowering did not alter pollen limitation in an early flowering shrub under short-term experimental warming
Authors: Pan Cheng-Chen , Feng Qi , Zhao Ha-Lin , Liu Lin-De , Li Yu-Lin , Li Yu-Qiang , Zhang Tong-Hui , Yu Xiao-Ya 
Published: May 2017

AbstractIn animal pollinated plants, phenological shifts caused by climate change may have important ecological consequences. However, no empirical evidence exists at present on the consequences that flowering phenology shifts have on the strength of pollen limitation under experimental warming. Here, we investigated the effects of experimental warming on flowering phenology, flower density, reproductive success, and pollen limitation intensity inCaragana microphyllaand evaluated whether earlier flowering phenology affected plant reproduction and the level of pollen limitation using warmed and unwarmed open top chambers in the Horqin Sandy Land of Inner Mongolia, northern China. The results of this study indicated that artificial warming markedly advanced flower phenology rather than extending the duration of the flowering. Additionally, warming was found to significantly reduce flower density which led to seed production reduction, since there were insignificant effects observed on fruit set and seed number per fruit. Experimental floral manipulations showed that warming did not affect pollen limitation. These results revealed the negative effects of advanced phenology induced by warming on flower density and reproductive output, as well as the neutral effects on reproductive success and pollen limitation intensity of long surviving plants.
Single-cell protein-mRNA correlation analysis enabled by multiplexed dual-analyte co-detection
Authors: Gong Haibiao , Wang Xiaohui , Liu Benjamin , Boutet Stephane , Holcomb Ilona , Dakshinamoorthy Gajalakshmi , Ooi Aik , Sanada Chad , Sun Gang , Ramakrishnan Ramesh 
Published: May 2017

AbstractWe have investigated the correlation between proteins and mRNAs in single cells employing an integrated workflow for dual-analyte co-detection. This is achieved by combining the oligo extension reaction (OER), which converts protein levels to DNA levels, with reverse transcription for mRNA detection. Unsupervised gene expression profiling analysis, including principal component analysis and hierarchical clustering, revealed different aspects of the protein-mRNA relationship. Violin plot analysis showed that some genes exhibited similar distribution patterns for proteins and mRNAs. We also demonstrate that cells can be separated into subpopulations based on their protein-mRNA expression profiles, and that different subpopulations have distinct correlation coefficient values. Our results demonstrated that integrated investigations of mRNA and protein levels in single cells allows comprehensive analysis not attainable at bulk levels.
The Effect of Interleukin 38 on Angiogenesis in a Model of Oxygen-induced Retinopathy
Authors: Zhang Jing , Zhao Ruijuan , Chen Jianping , Jin Jiayi , Yu Ying , Tian Yunzhe , Li Weihua , Wang Wencong , Zhou Hongyan , Bo Su Shao 
Published: May 2017

AbstractInterleukin 38 (IL-38) is a novel identified cytokine of IL-1 family in which some members are important in inflammation and angiogenesis. However, the role of IL-38 in regulating angiogenesis is unknown. The aim of the present study is to explore the effect of IL-38 on angiogenesis. Oxygen-induced retinopathy (OIR) of C57BL/6 J mice was induced by exposure of hyperoxia (75% oxygen) from postnatal day 7 (P7) to P12 and then returned to room air. The mice were injected with IL-38. At P17, neovascular region (tufts) and avascular area of the retinas were analyzed. The data showed that administration of IL-38 in vivo inhibited retinal angiogenesis significantly. Furthermore, the addition of IL-38 to the cell cultures attenuated the proliferation, scratch wound healing and tube formation of vascular endothelial cells induced by VEGF significantly. Our findings suggest that IL-38 is an antiangiogenic cytokine in pathophysiological settings and may have therapeutic potential for angiogenesis related diseases.