bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multicenter observational study assessing the safety, feasibility, and complications of Bonastent in central airway obstruction
Authors: Madisi Nagendra Y. , Ali Sana , Greenberg Daniel , Kobbari Gowthami , Salick Muhammad , Parimi Anoosh , Boujaoude Ziad , Abouzgheib Wissam 
Published: September 2024

Background: Self-expandable metallic stents (SEMS) are increasingly used in the management of both malignant and nonmalignant airway stenosis. There are multiple stents available in the market; however, the current literature on the efficacy and safety of newly available 3rd generation SEMS (Bonastent) is extremely limited and only has data from single center studies. Objectives: To report the efficacy and early (<7 days) and late (⩾7 days) complications in patients with central airway obstruction (CAO) treated with Bonastent placement at two institutions. Design: We performed a retrospective analysis of data of consecutive patients who underwent therapeutic bronchoscopy and Bonastent placement at two tertiary care university hospitals between January 2019 and November 2023. Methods: Bonastent deployment was performed in the operating room. Stents were deployed using rigid or flexible bronchoscopy under direct visualization with a flexible bronchoscope and in conjunction with fluoroscopic guidance. We then analyzed the effectiveness, short-term, and long-term complications of Bonastent placement. Results: A total of 107 Bonastents® were placed in 96 patients. The most common etiology of CAO was malignancy, 92.7% ( n = 89), followed by excessive dynamic airway collapse (EDAC) and post-intubation tracheal stenosis. Seventy-three patients (76%) had improvement in symptoms or imaging within 7 days of stent placement, including successful liberation from mechanical ventilation in a patient with CAO. Early complications occurred in seven patients (two-airway bleeding, two-mucus plugging that improved with airway clearance, two-stent migrations, and one-cough). Late complications occurred in 23 patients (1-stent migration requiring revision bronchoscopy and replacement of airway stent, 11-mucus plugging, 6-granulation tissue, 2-pneumonia, 1-cough, 1-tumor ingrowth/stent fracture, 1-airway emergency due to excessive granulation tissue obstructing the distal end of the stent and had a failed cricothyroidotomy leading to death). Overall, the early complication rate was 7.3% (7/96) and late complication rate was 23.9% (23/96). Conclusion: Our study is the first multicenter study that found a good safety profile with a low complication rate after tracheobronchial Bonastent placement with improvement in symptoms soon after stent placement.
Food Insecurity and Its Associated Factors Among Lactating Mothers in Yirgalem Town, South Ethiopia: A Community-Based Cross-Sectional Study
Authors: Endeshaw Fikremariam , W/kidan Haregwa Asnake , Zeleke Fentahun Tamene , Tefera Daniel Tsega , Tadesse Abebe Shimelis , Muhamed Ahmed Nuru , Kasahun Esuyawkal Mislu 
Published: September 2024

Background Food insecurity is a state in which people do not have both physical and economic access to sufficient food to meet their dietary needs. Food insecurity affects all nations despite that it is high in Africa, particularly in Ethiopia. There is limited information on the magnitude of food insecurity and its associated factors among lactating mothers in Ethiopia. Therefore, the purpose of this study was to determine the magnitude of food insecurity and associated factors among lactating mothers in Yirgalem Town, South Ethiopia. Methods A community-based cross-sectional study design was conducted on five randomly selected Kebeles and 557 lactating mothers who were selected using a simple random sampling technique from January 10 to April 30, 2022. The data were collected using interviewer administered questionnaires. Bi-variable and multivariable logistic regression analysis was used in the analysis. Family folder documentation was used to know the number of lactating mothers found in each Kebeles. Food insecurity was measured by household food insecurity access scale (version 3). Dietary diversity was assessed by the 24-hour dietary recall method. Results The magnitude of food insecurity among lactating mothers was 58.2%. Low minimum dietary diversity score, mothers with two and below antenatal care, mothers who had more than two children under 5 years, no home gardening practice, and having meals two and below were significantly associated with food insecurity. Conclusion The magnitude of food insecurity was 58.2% in the study area. Therefore, the agricultural and health sectors should work collaboratively to improve home gardening practices, dietary diversity practices and maternal health services are highly recommended to reduce food insecurity.
Evolution of unexpected diversity in a putative mating type locus and its correlation with genome variability reveals likely asexuality in the model mycorrhizal fungus Rhizophagus irregularis
Authors: Lee Soon-Jae , Risse Eric , Mateus Ivan D. , Sanders Ian R. 
Published: September 2024

Abstract
Background
Arbuscular mycorrhizal fungi (AMF) form mutualistic partnerships with approximately 80% of plant species. AMF, and their diversity, play a fundamental role in plant growth, driving plant diversity, and global carbon cycles. Knowing whether AMF are sexual or asexual has fundamental consequences for how they can be used in agricultural applications. Evidence for and against sexuality in the model AMF, Rhizophagus irregularis, has been proposed. The discovery of a putative mating-type locus (MAT locus) in R. irregularis, and the previously suggested recombination among nuclei of a dikaryon R. irregularis isolate, potentially suggested sexuality. Unless undergoing frequent sexual reproduction, evolution of MAT-locus diversity is expected to be very low. Additionally, in sexual species, MAT-locus evolution is decoupled from the evolution of arbitrary genome-wide loci.

Results
We studied MAT-locus diversity of R. irregularis. This was then compared to diversification in a phosphate transporter gene (PTG), that is not involved in sex, and to genome-wide divergence, defined by 47,378 single nucleotide polymorphisms. Strikingly, we found unexpectedly high MAT-locus diversity indicating that either it is not involved in sex, or that AMF are highly active in sex. However, a strongly congruent evolutionary history of the MAT-locus, PTG and genome-wide arbitrary loci allows us to reject both the hypothesis that the MAT-locus is involved in mating and that the R. irregularis lineage is sexual.

Conclusion
Our finding shapes the approach to developing more effective AMF strains and is highly informative as it suggests that introduced strains applied in agriculture will not exchange DNA with native populations.
Relationship between p16/ki67 immunoscores and PAX1/ZNF582 methylation status in precancerous and cancerous cervical lesions in high-risk HPV-positive women
Authors: Luo Haijun , Lian Yixiang , Tao Hong , Zhao Yan , Wang Zhigan , Zhou Jing , Zhang Zirong , Jiang Shali 
Published: September 2024

Abstract
Background
The risk of cervical cancer progression in high-risk human papillomavirus (HR-HPV)-positive women is associated with cervical lesion severity and molecular heterogeneity. Classification systems based on p16 and Ki67 expression cumulative scores (0–3 each)—p16/Ki67 collectively known as an immunoscore [IS]—are an accurate and reproducible method for grading cervical intraepithelial neoplasia (CIN) lesions. Meanwhile, DNA methylation is an early event in the development of cervical cancer. Hence, this study evaluated the relationship among CIN, p16/Ki-67 IS, and PAX1/ZNF582 methylation.

Methods
In this study, 414 HPV-positive paraffin-embedded specimens were collected, and PAX1/ZNF582 methylation and the p16/ki67 IS were determined. A total of 43 invalid samples were excluded and 371 were included in the statistical analyses. There were 103 cervicitis, 95 CIN1, 71 CIN2, 89 CIN3, and 13 squamous cell carcinoma (SCC) cases. The association between PAX1/ZNF582 methylation and p16/Ki6 immunohistochemical staining scores was analyzed.

Results
The ΔCp of PAX1m (PAX1 methylation) and ZNF582m (ZNF582 methylation) decreased with cervical lesion severity (Cuzick trend test, all P < 0.001). The severity of the cervical lesions and p16, Ki67, and p16/Ki67 IS showed an increasing trend (Multinomial Cochran-Armitage trend test, all P < 0.001). The prevalence of PAX1m/ZNF582m increased with an increase in the IS of p16, Ki67, and p16/Ki67 (Cochran-Armitage trend test, all P < 0.001). In cervical SCC, the IS was 5–6, and the PAX1m/ZNF582m was positive. Meanwhile, heterogeneity was observed in CIN lesions: 10 cases had an IS of 3–4 and were PAX1m/ZNF582m-positive in ≤ CIN1; 1 case had an IS of 0–2 and was PAX1m/ZNF582m-positive in CIN2/3.

Conclusions
Significant heterogeneity was observed in CIN lesions for p16 and Ki67 immunohistochemical staining scores and PAX1/ZNF582 methylation. This may help clinicians personalize the management of CIN based on the predicted short-term risk of cancer progression, minimizing the rate of missed CIN1 diagnoses and incorrect treatment of CIN2/3.
Patterns and perceptions of vaping among adults living in social housing: a representative survey in Great Britain, 2023
Authors: Jackson Sarah E , Brown Jamie , Lewer Dan , Cox Sharon 
Published: September 2024

Abstract
Background
Vaping products are effective for helping people to stop smoking and may therefore offer a potential means to reduce high rates of smoking in socioeconomically disadvantaged groups. This study aimed to examine current patterns and perceptions of vaping among people living in social housing in Great Britain compared with those living in other housing types.

Methods
Data were from the Smoking Toolkit Study; a nationally-representative survey conducted in 2023 (n = 23,245). Logistic regression tested cross-sectional associations between living in social (vs. other) housing and current vaping among adults; vaping frequency, device type, nicotine concentration, and source of purchase among current vapers; use of vaping products as a smoking cessation aid among past-year smokers who tried to quit; and harm perceptions of vaping products relative to cigarettes among current smokers.

Results
Current vaping prevalence was twice as high among adults living in social housing (19.4%) compared with those in other housing types (10.4%; OR = 2.07, 95%CI = 1.84–2.33). This was partly explained by differences in sociodemographic characteristics and smoking status; after adjustment, the odds of being a current vaper were 33% higher (ORadj=1.33, 95%CI = 1.14–1.54). Among vapers, there were no notable differences by housing tenure in vaping frequency, main device type used, usual nicotine concentration, usual source of purchase, or use as a smoking cessation aid. However, current smokers living in social housing were more likely to think vaping is more harmful than cigarettes (31.6% vs. 21.8%; ORadj=1.61, 95%CI = 1.30–1.99).

Conclusions
In Great Britain, adults who live in social housing are more likely to vape than those who live in other housing types, even after accounting for their younger age and higher smoking rates. However, misperceptions about the relative harms of vaping products and tobacco are common among smokers living in social housing. Interventions addressing these misperceptions could help encourage more people living in social housing to switch from smoking to vaping and reduce smoking-related health inequalities.

Pre-registration
The study protocol and analysis plan were pre-registered on Open Science Framework (https://osf.io/n3mvs/).
Examining the relationship between smartphone characteristics and the prevalence of hand discomfort among university students
Authors: Rahimian Benyamin , Banadaki Faeze Dehghan , Moraveji Fatemeh , Varmazyar Sakineh 
Published: September 2024

Abstract
Background
Students are among the groups that use smartphones for long periods throughout the day and night. Therefore, this study aimed to examine the relationship between smartphone characteristics and the prevalence of hand discomfort among university students.

Methods
This study included 204 university students, selected based on their willingness to participate and inclusion criteria. Participants reported hand pain and discomfort by completing the Cornell Hand Discomfort Questionnaire (CHDQ). Personal information was collected through a demographic questionnaire. Smartphone characteristics were obtained from the Internet based on the smartphone model self-reported by students.

Results
According to the Cornell questionnaire, 59.3% of students reported experiencing discomfort in their right hand, while 38.2% reported discomfort in their left hand due to smartphone use. Furthermore, 36.3% of students reported experiencing pain in two or more regions on their right hand, while 20.1% reported pain in two or more areas on their left hand. More than half of the students in the right hand (53.5%) and more than one-third (33.3%) in the left hand obtained pain scores of more than 1.5. The chi-square test indicated a statistically significant relationship between the weight of the smartphone and the prevalence of discomfort in the right hand (χ2 = 4.80, p = 0.03). Furthermore, a statistically significant relationship was found between the discomfort or pain scores experienced in both hands and the number of painful areas in those hands (right hand: χ2 = 219.04, p = 0.00; left hand: χ2 = 213.13, p = 0.00).

Conclusions
Smartphone use can cause discomfort and pain in the hands of university students. The physical characteristics of the smartphone, such as its weight, play a significant role in contributing to right-hand-related pain among students. It is important to consider ergonomic factors in smartphone design and usage to reduce musculoskeletal problems among users, especially students.
Carbohydrate quality index and risk of non-alcoholic fatty liver disease in Iranian adults
Authors: Jahromi Mitra Kazemi , Saber Niloufar , Norouzzadeh Mostafa , Daftari Ghazal , Pourhabibi-Zarandi Fatemeh , Ahmadirad Hamid , Farhadnejad Hossein , Teymoori Farshad , Salehi-Sahlabadi Ammar , Mirmiran Parvin 
Published: September 2024

Abstract
Background/aim
In the current study, we aimed to assess the association of carbohydrate quality index (CQI) with the risk of non-alcoholic fatty liver disease (NAFLD) in Iranian adults.

Methods
This case-control study was conducted on 225 newly diagnosed NAFLD patients and 450 controls, aged 20–60 years. A food frequency questionnaire was used to calculate the CQI and its components, including fiber intake, glycemic index, whole grains: total grains ratio, and solid carbohydrates: total carbohydrates ratio. Multivariable logistic regression was used to estimate the odds ratio (OR) of NAFLD across the tertile of CQI and its components.

Results
The participant’s mean ± SD of body mass index and age were 26.8 ± 4.3 kg/m2 and 38.1 ± 8.8 years, respectively. The median (interquartile) CQI score in participants of the case and control groups was 20 (15–25) and 23 (18–28), respectively. In the multivariable-adjusted model, the risk of NAFLD decreased significantly across the tertiles of the CQI [(OR: 0.20; %95CI: 0.11–0.39), Ptrend <0.001)]. Also, the odds of NAFLD decreased across tertiles of solid carbohydrates to total carbohydrates ratio [(OR: 0.39; 95%CI: 0.22–0.69), Ptrend <0.001)]. However, a high dietary glycemic index (GI) was associated with increased odds of NAFLD [(OR:7.47; 95%CI: 3.89–14.33, Ptrend<0.001)]. There was no significant relationship between other CQI components, including fiber intake and whole grain/total grains and the risk of NAFLD.

Conclusions
Our results revealed that a diet with a high quality of carbohydrates, characterized by higher intakes of solid carbohydrates, whole grain, and low GI carbohydrates, can be related to a reduced risk of NAFLD.
An antimicrobial microneedle patch promotes functional healing of infected wounds through controlled release of adipose tissue-derived apoptotic vesicles
Authors: Ma Yue , Dong Jia , Li Maojiao , Du Xinya , Yan Zhengbin , Tian Weidong 
Published: September 2024

AbstractThe high incidence and mortality rates associated with acute and chronic wound infections impose a significant burden on global healthcare systems. In terms of the management of wound infection, the reconstruction and regeneration of skin appendages are essential for the recovery of mechanical strength and physiological function in the regenerated skin tissue. Novel therapeutic approaches are a requisite for enhancing the healing of infected wounds and promoting the regeneration of skin appendages. Herein, a novel antimicrobial microneedle patch has been fabricated for the transdermal controlled delivery of adipose tissue-derived apoptotic vesicles (ApoEVs-AT@MNP) for the treatment of infected wounds, which is expected to achieve high-quality scarless healing of the wound skin while inhibiting the bacteria in the infected wound. The microneedle patch (MNP) system possesses adequate mechanical strength to penetrate the skin, allowing the tips to remain inside tissue for continuous active release of biomolecules, and subsequently degrades safely within the host body. In vivo transplantation demonstrates that ApoEVs-AT@MNP not only inhibits bacterial proliferation in infected wounds but also significantly promotes effective and rapid scarless wound healing. Particularly noteworthy is the ability of ApoEVs-AT@MNP to promote the rapid formation of mature, evenly arranged hair follicles in infected wounds, observed as early as 8 days following implantation, which is essential for the restoration of skin function. This rapid development of skin appendages has not been reported this early in previous studies. Therefore, ApoEVs-AT@MNP has emerged as an excellent, painless, non-invasive, and highly promising treatment for infected wounds.
Novel thiazole-based cyanoacrylamide derivatives: DNA cleavage, DNA/BSA binding properties and their anticancer behaviour against colon and breast cancer cells
Authors: Barakat Karim , Ragheb Mohamed A. , Soliman Marwa H. , Abdelmoniem Amr M. , Abdelhamid Ismail A. 
Published: September 2024

AbstractA novel series of 2-cyano-3-(pyrazol-4-yl)-N-(thiazol-2-yl)acrylamide derivatives (3a–f) were synthesized using Knoevenagel condensation and characterized using various spectral tools. The weak nuclease activity of compounds (3a–f) against pBR322 plasmid DNA was greatly enhanced by irradiation at 365 nm. Compounds 3b and 3c, incorporating thienyl and pyridyl moieties, respectively, exhibited the utmost nuclease activity in degrading pBR322 plasmid DNA through singlet oxygen and superoxide free radicals’ species. Furthermore, compounds 3b and 3c affinities towards calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) were investigated using UV–Vis and fluorescence spectroscopic analysis. They revealed good binding characteristics towards CT-DNA with Kb values of 6.68 × 104 M−1 and 1.19 × 104 M−1 for 3b and 3c, respectively. In addition, compounds 3b and 3c ability to release free radicals on radiation were targeted to be used as cytotoxic compounds in vitro for colon (HCT116) and breast cancer (MDA-MB-231) cells. A significant reduction in the cell viability on illumination at 365 nm was observed, with IC50 values of 23 and 25 µM against HCT116 cells, and 30 and 9 µM against MDA-MB-231 cells for compounds 3b and 3c, respectively. In conclusion, compounds 3b and 3c exhibited remarkable DNA cleavage and cytotoxic activity on illumination at 365 nm which might be associated with free radicals’ production in addition to having a good affinity for interacting with CT-DNA and BSA.
Graphical Abstract
Porcine ex-vivo intestinal mucus has age-dependent blocking activity against transmissible gastroenteritis virus
Authors: Saleem Waqar , Carpentier Nathan , Hinnekens Charlotte , Oh Dayoung , Van Vlierberghe Sandra , Braeckmans Kevin , Nauwynck Hans 
Published: September 2024

AbstractTransmissible gastroenteritis virus (TGEV) causes high mortality in young piglets (< 3 days of age). With aging, the susceptibility/morbidity/mortality rates drop. We previously hypothesized that the age-related changes in the intestinal mucus could be responsible for this resistance. Hence, this study investigated the effect of porcine intestinal mucus from 3-day and 3-week-old pigs on the free mobility of the virulent TGEV Miller strain, and on the infection in swine testicle (ST) cells. Single particle tracking (SPT) revealed that TGEV had significantly higher diffusion coefficients in 3-day mucus compared to 3-week mucus. TGEV and charged and uncharged control nanoparticles diffused freely in 3-day mucus but were hindered by 3-week mucus in the diffusion model; TGEV mimicked the diffusion behavior of negatively charged carboxylated particles. Inoculation of ST cells with TGEV in the presence of 3-week mucus resulted in a significantly lower average number of infected cells (30.9 ± 11.9/5 fields) compared with 3-day mucus (84.6 ± 16.4/5 fields). These results show that 3-week mucus has a significant TGEV-blocking activity compared to 3-day mucus in free diffusion and infection of the underlying susceptible cells. Additionally, a label-free proteomics analysis revealed an increased expression of mucin 13, known for negatively regulating the tight junctions in intestinal epithelium, in 3-day-old pigs. In 3-week-old pigs, a higher expression of mucin 2, a type of secreted mucin which is known for inhibiting coronavirus infection, was observed. Concludingly, this study demonstrated a protective effect of 3-week mucus against viral infections.
Upcycling sugar beet waste into sustainable organo-nanocatalysis for carbon dioxide fixation and cyclic carbonate synthesis: a research design study
Authors: Moazen Fateme , Eshghi Hossein , Torabi Hossein 
Published: September 2024

AbstractEnvironmental pollution is a major global issue due to the increase of various pollutants all over the world. Enhancing pollutant remediation strategies for environmental sustainability necessitates increasing the efficiency of conventional methods or introducing innovative approaches. Nanotechnology, particularly carbon-based nanomaterials, offers substantial promise due to their high surface area and absorption potential. Concurrently, organocatalysts have emerged as sustainable and versatile alternatives to traditional metal-based catalysts in modern chemical research. This study highlights the synthesis and application of organo-nanocatalysts derived from biomass, specifically a spherical carbon nanocatalyst synthesized from sugar beet pulp. This novel green catalyst, characterized by high selectivity and efficiency, successfully converts epoxides and CO2 into valuable cyclic carbonates under solvent-free conditions. The hydroxyl groups on the Sugar Beet-derived Carbon NanoSphere (SCNS) surface act as Bronsted acid sites, facilitating epoxide activation via hydrogen bonding. The integration of carbon-based nanomaterials and organocatalysis represents a promising, sustainable solution for pollutant remediation and green chemistry advancements.
Graphical Abstract
Differential diagnosis of Guillain-Barré syndrome: steroid-responsive radiculopathy in Evans syndrome
Authors: Schulten Thomas , Meyer Ansgar , Krug Utz , Lehmann Helmar C. 
Published: September 2024

AbstractGuillain-Barré syndrome is the most common acute inflammatory demyelinating peripheral nerve condition. Occasionally, other autoimmune conditions can mimic Guillain-Barré syndrome but may require different diagnostic workup and treatment. We report here two patients with Evans syndrome, a rare hematological autoimmune condition who developed a subacute inflammatory radiculopathy. Similarities and distinguishing clinical and diagnostic features are discussed.