bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of warm‐up on hamstring muscles stiffness: Cycling vs foam rolling
Authors: Morales‐Artacho A. J. , Lacourpaille L. , Guilhem G. 
Published: January 2017

This study investigated the effects of active and/or passive warm‐up tasks on the hamstring muscles stiffness through elastography and passive torque measurements. On separate occasions, fourteen males randomly completed four warm‐up protocols comprising Control, Cycling, Foam rolling, or Cycling plus Foam rolling (Mixed). The stiffness of the hamstring muscles was assessed through shear wave elastography, along with the passive torque‐angle relationship and maximal range of motion (ROM) before, 5, and 30 minutes after each experimental condition. At 5 minutes, Cycling and Mixed decreased shear modulus (−10.3% ± 5.9% and −7.7% ± 8.4%, respectively; P≤.0003, effect size [ES]≥0.24) and passive torque (−7.17% ± 8.6% and −6.2% ± 7.5%, respectively; P≤.051, ES≥0.28), and increased ROM (+2.9% ± 2.9% and +3.2% ± 3.5%, respectively; P≤.001, ES≥0.30); 30 minutes following Mixed, shear modulus (P=.001, ES=0.21) and passive torque (P≤.068, ES≥0.2) were still slightly decreased, while ROM increased (P=.046, ES=0.24). Foam rolling induced “small” immediate short‐term decreases in shear modulus (−5.4% ± 5.7% at 5 minutes; P=.05, ES=0.21), without meaningful changes in passive torque or ROM at any time point (P≥.12, ES≤0.23). These results suggest that the combined warm‐up elicited no acute superior effects on muscle stiffness compared with cycling, providing evidence for the key role of active warm‐up to reduce muscle stiffness. The time between warm‐up and competition should be considered when optimizing the effects on muscle stiffness.
Executive function and theory of mind as predictors of aggressive and prosocial behavior and peer acceptance in early childhood
Authors: O'Toole Sarah E. , Monks Claire P. , Tsermentseli Stella 
Published: January 2017

AbstractExecutive function (EF) and theory of mind (ToM) are related to children's social interactions, such as aggression and prosocial behavior, as well as their peer acceptance. However, limited research has examined different forms of aggression and the moderating role of gender. This study investigated links between EF, ToM, physical and relational aggression, prosocial behavior and peer acceptance and explored whether these relations are gender specific. Children (N = 106) between 46‐ and 80‐months‐old completed tasks assessing cool and hot EF and ToM. Teaching staff rated children's aggression, prosocial behavior, and peer acceptance. EF and ToM predicted physical, but not relational, aggression. Poor inhibition and delay of gratification were uniquely associated with greater physical aggression. EF and ToM did not predict prosocial behavior or peer acceptance. Added to this, gender did not moderate the relation between either EF or ToM and social outcomes. The correlates of aggression may therefore differ across forms of aggression but not between genders in early childhood.
Airborne laser-guided imaging spectroscopy to map forest trait diversity and guide conservation
Authors: Asner G. P. , Martin R. E. , Knapp D. E. , Tupayachi R. , Anderson C. B. , Sinca F. , Vaughn N. R. , Llactayo W. 
Published: January 2017

Airborne spectroscopy for forest traitsThe development of conservation priorities in the tropics is often hampered by the sparseness of ground data on biological diversity and the relative crudeness of larger-scale remote sensing data. Asneret al.developed airborne instrumentation to make large-scale maps of forest functional diversity across 72 million hectares of the Peruvian Andes and Amazon basin (see the Perspective by Kapos). They generated a suite of forest canopy functional trait maps from laser-guided imaging spectroscopy and used them to define distinct forest functional classes. These were then compared with government deforestation and land allocation data, which enabled an analysis of conservation threats and opportunities across the region.Science, this issue p.385; see also p.347
Is Follow-Up Testing with the FilmArray Gastrointestinal Multiplex PCR Panel Necessary?
Authors: Park Sholhui , Hitchcock Matthew M. , Gomez Carlos A. , Banaei Niaz 
Published: January 2017

ABSTRACT

The FilmArray gastrointestinal (GI) panel (BioFire Diagnostics, Salt Lake City, UT) is a simple, sample-to-answer, on-demand, multiplex, nucleic acid amplification test for syndromic diagnosis of infectious gastroenteritis. The aim of this study was to measure the yield of follow-up testing with FilmArray GI panel within 4 weeks of an initial test. Consecutive adult and pediatric patients tested at an academic institution between August 2015 and June 2016 were included in this study. Of 145 follow-up tests in 106 unique patients with an initial negative result, 134 (92.4%) tests and 98 (92.5%) patients remained negative upon follow-up testing. Excluding targets that are not reported at this institution (
Clostridium difficile
, enteroaggregative
Escherichia coli
, enteropathogenic
E. coli
, and enterotoxigenic
E. coli
), 137 (94.5%) follow-up tests and 101 (95.3%) patients remained negative. Weekly conversion rates were not significantly different across the 4-week follow-up interval. No epidemiological or clinical factors were significantly associated with a negative to positive conversion. Of 80 follow-up tests in patients with an initial positive result, 43 (53.8%) remained positive for the same target, 34 (42.5%) were negative, and 3 were positive for a different target (3.8%). Follow-up testing with FilmArray GI panel within 4 weeks of a negative result rarely changed the initial result, and the follow-up test reverted to negative less than half the time after an initial positive result. In the absence of clinical or epidemiological evidence for a new infection, follow-up testing should be limited and FilmArray GI panel should not be used as a test of cure.
Matrix Metalloproteinase 9 Facilitates Hepatitis B Virus Replication through Binding with Type I Interferon (IFN) Receptor 1 To Repress IFN/JAK/STAT Signaling
Authors: Chen Junbo , Xu Wei , Chen Yanni , Xie Xueping , Zhang Yecheng , Ma Chunqiang , Yang Qingyu , Han Yang , Zhu Chengliang , Xiong Ying , Wu Kailang , Liu Fang , Liu Yingle , Wu Jianguo 
Published: January 2017

ABSTRACT
Hepatitis B virus (HBV) infection may cause acute hepatitis B, chronic hepatitis B (CHB), liver cirrhosis, and hepatocellular carcinoma (HCC). However, the mechanisms by which HBV evades host immunity and maintains chronic infection are largely unknown. Here, we revealed that matrix metalloproteinase 9 (MMP-9) is activated in peripheral blood mononuclear cells (PBMCs) of HBV-infected patients, and HBV stimulates MMP-9 expression in macrophages and PBMCs isolated from healthy individuals. MMP-9 plays important roles in the breakdown of the extracellular matrix and in the facilitation of tumor progression, invasion, metastasis, and angiogenesis. MMP-9 also regulates respiratory syncytial virus (RSV) replication, but the mechanism underlying such regulation is unknown. We further demonstrated that MMP-9 facilitates HBV replication by repressing the interferon (IFN)/Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway, IFN action, STAT1/2 phosphorylation, and IFN-stimulated gene (ISG) expression. Moreover, MMP-9 binds to type I IFN receptor 1 (IFNAR1) and facilitates IFNAR1 phosphorylation, ubiquitination, subcellular distribution, and degradation to interfere with the binding of IFANR1 to IFN-α. Thus, we identified a novel positive-feedback regulation loop between HBV replication and MMP-9 production. On one hand, HBV activates MMP-9 in infected patients and leukocytes. On the other hand, MMP-9 facilitates HBV replication through repressing IFN/JAK/STAT signaling, IFNAR1 function, and IFN-α action. Therefore, HBV may take the advantage of MMP-9 function to establish or maintain chronic infection.

IMPORTANCE
Hepatitis B virus (HBV) infection may cause chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC). However, the mechanisms by which HBV maintains chronic infection are largely unknown. Matrix metalloproteinase 9 (MMP-9) plays important roles in the facilitation of tumor progression, invasion, metastasis, and angiogenesis. However, the effects of MMP-9 on HBV replication and pathogenesis are not known. This study reveals that MMP-9 expression is activated in patients with CHB, and HBV stimulates MMP-9 production in PBMCs and macrophages. More interestingly, MMP-9 in turn promotes HBV replication through suppressing IFN-α action. Moreover, MMP-9 interacts with type I interferon receptor 1 (IFNAR1) to disturb the binding of IFN-α to IFNAR1 and facilitate the phosphorylation, ubiquitination, subcellular distribution, and degradation of IFNAR1. Therefore, these results discover a novel role of MMP-9 in viral replication and reveal a new mechanism by which HBV evades host immunity to maintain persistent infection.
Nucleic and Amino Acid Sequences Support Structure-Based Viral Classification
Authors: Sinclair Robert M. , Ravantti Janne J. , Bamford Dennis H. 
Published: January 2017

ABSTRACT
Viral capsids ensure viral genome integrity by protecting the enclosed nucleic acids. Interactions between the genome and capsid and between individual capsid proteins (i.e., capsid architecture) are intimate and are expected to be characterized by strong evolutionary conservation. For this reason, a capsid structure-based viral classification has been proposed as a way to bring order to the viral universe. The seeming lack of sufficient sequence similarity to reproduce this classification has made it difficult to reject structural convergence as the basis for the classification. We reinvestigate whether the structure-based classification for viral coat proteins making icosahedral virus capsids is in fact supported by previously undetected sequence similarity. Since codon choices can influence nascent protein folding cotranslationally, we searched for both amino acid and nucleotide sequence similarity. To demonstrate the sensitivity of the approach, we identify a candidate gene for the pandoravirus capsid protein. We show that the structure-based classification is strongly supported by amino acid and also nucleotide sequence similarities, suggesting that the similarities are due to common descent. The correspondence between structure-based and sequence-based analyses of the same proteins shown here allow them to be used in future analyses of the relationship between linear sequence information and macromolecular function, as well as between linear sequence and protein folds.

IMPORTANCE
Viral capsids protect nucleic acid genomes, which in turn encode capsid proteins. This tight coupling of protein shell and nucleic acids, together with strong functional constraints on capsid protein folding and architecture, leads to the hypothesis that capsid protein-coding nucleotide sequences may retain signatures of ancient viral evolution. We have been able to show that this is indeed the case, using the major capsid proteins of viruses forming icosahedral capsids. Importantly, we detected similarity at the nucleotide level between capsid protein-coding regions from viruses infecting cells belonging to all three domains of life, reproducing a previously established structure-based classification of icosahedral viral capsids.
The Pseudorabies Virus Glycoprotein gE/gI Complex Suppresses Type I Interferon Production by Plasmacytoid Dendritic Cells
Authors: Lamote Jochen A. S. , Kestens Manon , Van Waesberghe Cliff , Delva Jonas , De Pelsmaeker Steffi , Devriendt Bert , Favoreel Herman W. 
Published: January 2017

ABSTRACTPlasmacytoid dendritic cells (pDC) play a central role in the antiviral immune response, both in the innate response and in shaping the adaptive response, mainly because of their ability to produce massive amounts of type I interferon (TI-IFN). Here, we report that cells infected with the live attenuated Bartha vaccine strain of porcine alphaherpesvirus pseudorabies virus (PRV) trigger a dramatically increased TI-IFN response by porcine primary pDC compared to cells infected with wild-type PRV strains (Becker and Kaplan). Since Bartha is one of the relatively few examples of a highly successful alphaherpesvirus vaccine, identification of factors that may contribute to its efficacy may provide insights for the rational design of other alphaherpesvirus vaccines. The Bartha vaccine genome displays several mutations compared to the genome of wild-type PRV strains, including a large deletion in the unique short (US) region, encompassing the glycoprotein E (gE), gI, US9, and US2 genes. Using recombinant PRV Becker strains harboring the entire Bartha US deletion or single mutations in the four affected US genes, we demonstrate that the absence of the viral gE/gI complex contributes to the observed increased IFN-α response. Furthermore, we show that the absence of gE leads to an enhanced extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in pDC, which correlates with a higher TI-IFN production by pDC. In conclusion, the PRV Bartha vaccine strain triggers strongly increased TI-IFN production by porcine pDC. Our data further indicate that the gE/gI glycoprotein complex suppresses TI-IFN production by pDC, which represents the first alphaherpesvirus factor that suppresses pDC activity.IMPORTANCESeveral alphaherpesviruses, including herpes simpex virus, still lack effective vaccines. However, the highly successful Bartha vaccine has contributed substantially to eradication of the porcine alphaherpesvirus pseudorabies virus (PRV) in several countries. The impact of Bartha on the immune response is still poorly understood. Type I interferon (TI-IFN)-producing plasmacytoid dendritic cells (pDC) may play an important role in vaccine development. Here, we show that Bartha elicits a dramatically increased type I interferon (TI-IFN) response in primary porcine pDC compared to wild-type strains. In addition, we found that the gE/gI complex, which is absent in Bartha, inhibits the pDC TI-IFN response. This is the first description of an immune cell type that is differentially affected by Bartha versus wild-type PRV and is the first report describing an alphaherpesvirus protein that inhibits the TI-IFN response by pDC. These data may therefore contribute to the rational design of other alphaherpesvirus vaccines.
Impact of Type I Interferon on the Safety and Immunogenicity of an Experimental Live-Attenuated Herpes Simplex Virus 1 Vaccine in Mice
Authors: Royer Derek J. , Carr Meghan M. , Chucair-Elliott Ana J. , Halford William P. , Carr Daniel J. J. 
Published: January 2017

ABSTRACTViral fitness dictates virulence and capacity to evade host immune defenses. Understanding the biological underpinnings of such features is essential for rational vaccine development. We have previously shown that the live-attenuated herpes simplex virus 1 (HSV-1) mutant lacking the nuclear localization signal (NLS) on the ICP0 gene (0ΔNLS) is sensitive to inhibition by interferon beta (IFN-β)in vitroand functions as a highly efficacious experimental vaccine. Here, we characterize the host immune response andin vivopathogenesis of HSV-1 0ΔNLS relative to its fully virulent parental strain in C57BL/6 mice. Additionally, we explore the role of type 1 interferon (IFN-α/β) signaling on virulence and immunogenicity of HSV-1 0ΔNLS and uncover a probable sex bias in the induction of IFN-α/β in the cornea during HSV-1 infection. Our data show that HSV-1 0ΔNLS lacks neurovirulence even in highly immunocompromised mice lacking the IFN-α/β receptor. These studies support the translational viability of the HSV-1 0ΔNLS vaccine strain by demonstrating that, while it is comparable to a virulent parental strain in terms of immunogenicity, HSV-1 0ΔNLS does not induce significant tissue pathology.IMPORTANCEHSV-1 is a common human pathogen associated with a variety of clinical presentations ranging in severity from periodic “cold sores” to lethal encephalitis. Despite the consistent failures of HSV subunit vaccines in clinical trials spanning the past 28 years, opposition to live-attenuated HSV vaccines predicated on unfounded safety concerns currently limits their widespread acceptance. Here, we demonstrate that a live-attenuated HSV-1 vaccine has great translational potential.
Determinants of weight change in patients on basal insulin treatment: an analysis of the DIVE registry
Authors: Bramlage Peter , Bluhmki Tobias , Fleischmann Holger , Kaltheuner Matthias , Beyersmann Jan , Holl Reinhard W , Danne Thomas 
Published: January 2017

ObjectiveWe aimed to describe patterns of weight change in insulin-naive patients with type 2 diabetes mellitus (T2DM) starting basal insulin (BI) treatment.Research design and methodsDiabetes Versorgungs-Evaluation (DIVE) is an observational, multicenter, prospective registry in patients with T2DM. Patients were divided into those initiating BI therapy for the first time (with optional oral antidiabetic drugs (OADs)) and those initiating OADs only (OADo).Results521 patients were included in the analysis, 113 in the BI arm and 408 in the OADo arm. Relative to baseline, the BI group gained an average of 0.98±7.1 kg at 1 year, compared with a loss of 1.52±11.8 kg in the OADo group (p<0.001). This difference remained statistically significant when expressed as a proportional change from baseline (+0.014±0.08 vs −0.015±0.12, respectively (p<0.001)). Baseline weight (regression coefficient (RC) 0.89; 95% CI 0.81 to 0.97; p<0.001) and diabetes duration (RC 2.52; 95% CI 0.53 to 4.52; p=0.01) were the only factors identified as significant predictors of weight gain between baseline and 1 year follow-up in BI patients.ConclusionsThough BI therapy leads to modest weight gain over the subsequent year, this may be limited by BI initiation at an early stage of the disease. As such, delaying the start of insulin therapy based on fears of weight gain appears counter-productive, and should be reconsidered.
Quality of recording of diabetes in the UK: how does the GP's method of coding clinical data affect incidence estimates? Cross-sectional study using the CPRD database
Authors: Tate A Rosemary , Dungey Sheena , Glew Simon , Beloff Natalia , Williams Rachael , Williams Tim 
Published: January 2017

ObjectiveTo assess the effect of coding quality on estimates of the incidence of diabetes in the UK between 1995 and 2014.DesignA cross-sectional analysis examining diabetes coding from 1995 to 2014 and how the choice of codes (diagnosis codes vs codes which suggest diagnosis) and quality of coding affect estimated incidence.SettingRoutine primary care data from 684 practices contributing to the UK Clinical Practice Research Datalink (data contributed from Vision (INPS) practices).Main outcome measureIncidence rates of diabetes and how they are affected by (1) GP coding and (2) excluding ‘poor’ quality practices with at least 10% incident patients inaccurately coded between 2004 and 2014.ResultsIncidence rates and accuracy of coding varied widely between practices and the trends differed according to selected category of code. If diagnosis codes were used, the incidence of type 2 increased sharply until 2004 (when the UK Quality Outcomes Framework was introduced), and then flattened off, until 2009, after which they decreased. If non-diagnosis codes were included, the numbers continued to increase until 2012. Although coding quality improved over time, 15% of the 666 practices that contributed data between 2004 and 2014 were labelled ‘poor’ quality. When these practices were dropped from the analyses, the downward trend in the incidence of type 2 after 2009 became less marked and incidence rates were higher.ConclusionsIn contrast to some previous reports, diabetes incidence (based on diagnostic codes) appears not to have increased since 2004 in the UK. Choice of codes can make a significant difference to incidence estimates, as can quality of recording. Codes and data quality should be checked when assessing incidence rates using GP data.
Unusual urachal remnant disease in a six‐month‐old golden retriever
Authors: Sebastian Marcos Patricia , Andaluz Anna , Planellas Bachs Marta 
Published: January 2017

A six‐month‐old male entire golden retriever was presented with an umbilical mass noted by the owners four days before. Clinical examination revealed fever (39.7°C) and an umbilical fistulised abscess. Abdominal palpation revealed a solid mass in the caudal abdomen. The ultrasonographic study confirmed the presence of an abdominal mass (7 cm in diameter) adhered to several abdominal organs, mild localised peritonitis and an umbilical defect. The mass was compatible with an abscess or a granuloma potentially associated with an urachal remnant. An exploratory laparotomy confirmed the suspicion. The mass was removed and the urachal remnant corrected. Histopathology revealed the presence of a granuloma with multiple necrotic areas and basophilic structures suggestive of bacterial infection. Few days after surgery, all clinical signs resolved completely. Urachal remnants are rarely reported in small animals. To the authors’ knowledge, this is the first report describing such a severe presentation with complete resolution.
Hiding Yourself Behind Collaborative Users When Using Continuous Location-Based Services
Authors: Ma Chunguang , Zhang Lei , Yang Songtao , Zheng Xiaodong 
Published: January 2017

The prosperity of location-based services (LBSs) makes more and more people pay close attention to personal privacy. In order to preserve users privacy, several schemes utilized a trusted third party (TTP) to obfuscate users, but these schemes were suspected as the TTP may become the single point of failure or service performance bottleneck. To alleviate the suspicion, schemes with collaborative users to achieve [Formula: see text]-anonymity were proposed. In these schemes, users equipped with short-range communication devices could communicate with adjacent users to establish an anonymous group. With this group, the user can obfuscate and hide herself behind at least [Formula: see text] other users. However, these schemes are usually more efficient in snapshot services than continuous ones. To cope with the inadequacy, with the help of caching in mobile devices, we propose a query information blocks random exchange and results caching scheme (short for CaQBE). In this scheme, a particular user is hidden behind collaborative users in snapshot service, and then the caches further preserve the privacy in continuous service. In case of the active adversary launching the query correlation attack and the passive adversary launching the impersonation attack, a random collaborative user selection and a random block exchange algorithm are also utilized. Then based on the feature of entropy, a metric to measure the privacy of the user against attacks from the active and passive adversaries is proposed. Finally, security analysis and experimental comparison with other similar schemes further verify the optimal of our scheme in effectiveness of preservation and efficiency of performance.