bluuebunny/crossref_metadata_2025_split huggingface dataset:
SK4 channels modulate Ca2+ signalling and cell cycle progression in murine breast cancer
Authors: Steudel Friederike A. , Mohr Corinna J. , Stegen Benjamin , Nguyen Hoang Y. , Barnert Andrea , Steinle Marc , Beer‐Hammer Sandra , Koch Pierre , Lo Wing‐Yee , Schroth Werner , Hoppe Reiner , Brauch Hiltrud , Ruth Peter , Huber Stephan M. , Lukowski Robert 
Published: May 2017

Oncogenic signalling via Ca2+‐activated K+ channels of intermediate conductance (SK4, also known as KCa3.1 or IK) has been implicated in different cancer entities including breast cancer. Yet, the role of endogenous SK4 channels for tumorigenesis is unclear. Herein, we generated SK4‐negative tumours by crossing SK4‐deficient (SK4 KO) mice to the polyoma middle T‐antigen (PyMT) and epidermal growth factor receptor 2 (cNeu) breast cancer models in which oncogene expression is driven by the retroviral promoter MMTV. Survival parameters and tumour progression were studied in cancer‐prone SK4 KO in comparison with wild‐type (WT) mice and in a syngeneic orthotopic mouse model following transplantation of SK4‐negative or WT tumour cells. SK4 activity was modulated by genetic or pharmacological means using the SK4 inhibitor TRAM‐34 in order to establish the role of breast tumour SK4 for cell growth, electrophysiological signalling, and [Ca2+]i oscillations. Ablation of SK4 and TRAM‐34 treatment reduced the SK4‐generated current fraction, growth factor‐dependent Ca2+ entry, cell cycle progression and the proliferation rate of MMTV‐PyMT tumour cells. In vivo, PyMT oncogene‐driven tumorigenesis was only marginally affected by the global lack of SK4, whereas tumour progression was significantly delayed after orthotopic implantation of MMTV‐PyMT SK4 KO breast tumour cells. However, overall survival and progression‐free survival time in the MMTV‐cNeu mouse model were significantly extended in the absence of SK4. Collectively, our data from murine breast cancer models indicate that SK4 activity is crucial for cell cycle control. Thus, the modulation of this channel should be further investigated towards a potential improvement of existing antitumour strategies in human breast cancer.
Anger Management
Authors: Glancy Graham , Saini Michael , Treffers Stefan 
Published: May 2017

Abstract
Chronic maladaptive anger has been associated with a number of adverse outcomes, including reductions in overall mental and physical health, deterioration of interpersonal relations, and legal consequences arising from anger expressed in the form of aggressive and violent behavior. Despite a plethora of treatment modalities, there has been little consensus on the most effective treatments for chronic maladaptive anger. The aim of this chapter is to provide a brief summary of the empirical evidence associated with a range of psychological and pharmacological interventions to help guide administration of anger management and treatment programs.
High‐Throughput Inertial Focusing of Micrometer‐ and Sub‐Micrometer‐Sized Particles Separation
Authors: Wang Lei , Dandy David S. 
Published: May 2017

AbstractThe ability to study individual bacteria or subcellular organelles using inertial microfluidics is still nascent. This is due, in no small part, to the significant challenges associated with concentrating and separating specific sizes of micrometer and sub‐micrometer bioparticles in a microfluidic format. In this study, using a rigid polymeric microfluidic network with optimized microchannel geometry dimensions, it is demonstrated that 2 µm, and even sub‐micrometer, particles can be continuously and accurately focused to stable equilibrium positions. Suspensions have been processed at flow rates up to 1400 µL min−1 in an ultrashort 4 mm working channel length. A wide range of suspension concentrations—from 0.01 to 1 v/v%—have been systematically investigated, with yields greater than 97%, demonstrating the potential of this technology for large‐scale implementation. Additionally, the ability of this chip to separate micrometer‐ and sub‐micrometer‐sized particles and to focus bioparticles (cyanobacteria) has been demonstrated. This study pushes the microfluidic inertial focusing particle range down to sub‐micrometer length scales, enabling novel routes for investigation of individual microorganisms and subcellular organelles.
Supersäuren basierend auf Pentafluoroorthotelluraten des Aluminiums
Authors: Wiesner Anja , Gries Thomas W. , Steinhauer Simon , Beckers Helmut , Riedel Sebastian 
Published: May 2017

AbstractDie neue Lewis‐Säure Al(OTeF5)3 und sein Acetonitril‐Addukt wurden mit Hilfe einer einfachen Syntheseroute in Mengen bis zu 15 g hergestellt und charakterisiert (IR‐, Raman‐Spektroskopie, DFT‐Rechnungen). Ausgehend von der Brønsted‐Säure H[Al(OTeF5)4] wurden mittels Protonierung fünf verschiedene Salze des schwach koordinierenden Anions [Al(OTeF5)4]− in einer zweistufigen Synthese synthetisiert. [Ph4P][Al(OTeF5)4], Cs[Al(OTeF5)4], [Ph3C][Al(OTeF5)4] sowie die protonierten Benzolderivate [C9H13][Al(OTeF5)4] und [C6H7][Al(OTeF5)4] wurden mittels Einkristallröntgenstrukturanalyse und ihrer NMR‐Spektren charakterisiert. Festkörperstrukturen solcher Areniumionen sind selten und wurden in dieser Arbeit auf einem einfachen Weg hergestellt.
Chemically Tailoring the Dopant Emission in Manganese‐Doped CsPbCl3 Perovskite Nanocrystals
Authors: Das Adhikari Samrat , Dutta Sumit K. , Dutta Anirban , Guria Amit K. , Pradhan Narayan 
Published: May 2017

AbstractDoping in perovskite nanocrystals adopts different mechanistic approach in comparison to widely established doping in chalcogenide quantum dots. The fast formation of perovskites makes the dopant insertions more competitive and challenging. Introducing alkylamine hydrochloride (RNH3Cl) as a promoting reagent, precise controlled doping of MnII in CsPbCl3 perovskite nanocrystals is reported. Simply, by changing the amount of RNH3Cl, the Mn incorporation and subsequent tuning in the excitonic as well as Mn d–d emission intensities are tailored. Investigations suggested that RNH3Cl acted as the chlorinating source, controlled the size, and also helps in increasing the number of particles. This provided more opportunity for Mn ions to take part in reaction and occupied the appropriate lattice positions. Carrying out several reactions with varying reaction parameters, the doping conditions are optimized and the role of the promoting reagent for both doped and undoped systems are compared.
AgHalo: A Facile Fluorogenic Sensor to Detect Drug‐Induced Proteome Stress
Authors: Liu Yu , Fares Matthew , Dunham Noah P. , Gao Zi , Miao Kun , Jiang Xueyuan , Bollinger Samuel S. , Boal Amie K. , Zhang Xin 
Published: May 2017

AbstractDrug‐induced proteome stress that involves protein aggregation may cause adverse effects and undermine the safety profile of a drug. Safety of drugs is regularly evaluated using cytotoxicity assays that measure cell death. However, these assays provide limited insights into the presence of proteome stress in live cells. A fluorogenic protein sensor is reported to detect drug‐induced proteome stress prior to cell death. An aggregation prone Halo‐tag mutant (AgHalo) was evolved to sense proteome stress through its aggregation. Detection of such conformational changes was enabled by a fluorogenic ligand that fluoresces upon AgHalo forming soluble aggregates. Using 5 common anticancer drugs, we exemplified detection of differential proteome stress before any cell death was observed. Thus, this sensor can be used to evaluate drug safety in a regime that the current cytotoxicity assays cannot cover and be generally applied to detect proteome stress induced by other toxins.
Double‐Layered Plasmonic–Magnetic Vesicles by Self‐Assembly of Janus Amphiphilic Gold–Iron(II,III) Oxide Nanoparticles
Authors: Song Jibin , Wu Binghui , Zhou Zijian , Zhu Guizhi , Liu Yijing , Yang Zhen , Lin Lisen , Yu Guocan , Zhang Fuwu , Zhang Guofeng , Duan Hongwei , Stucky Galen D. , Chen Xiaoyuan 
Published: May 2017

AbstractJanus nanoparticles (JNPs) offer unique features, including the precisely controlled distribution of compositions, surface charges, dipole moments, modular and combined functionalities, which enable excellent applications that are unavailable to their symmetrical counterparts. Assemblies of NPs exhibit coupled optical, electronic and magnetic properties that are different from single NPs. Herein, we report a new class of double‐layered plasmonic–magnetic vesicle assembled from Janus amphiphilic Au‐Fe3O4 NPs grafted with polymer brushes of different hydrophilicity on Au and Fe3O4 surfaces separately. Like liposomes, the vesicle shell is composed of two layers of Au‐Fe3O4 NPs in opposite direction, and the orientation of Au or Fe3O4 in the shell can be well controlled by exploiting the amphiphilic property of the two types of polymers.
Phosphoribosyl Pyrophosphate: A Molecular Vestige of the Origin of Life on Minerals
Authors: Akouche Mariame , Jaber Maguy , Maurel Marie‐Christine , Lambert Jean‐Francois , Georgelin Thomas 
Published: May 2017

AbstractIn this contribution, we report the formation under prebiotic conditions of phosphoribosyl pyrophosphate (PRPP) as a molecular precursor in the one‐pot synthesis of a canonical nucleotide, namely adenosine monophosphate (AMP) from its building blocks (KH2PO4 or Pi, adenine, and d‐ribose), on a fumed silica surface. The on‐the‐rocks approach has been successfully applied to the simultaneous phosphorylation and glycosylation of ribose. The one‐pot formation mechanism of AMP involves a two‐step pathway via an activated intermediate, namely PRPP, obtained by multiple ribose phosphorylations upon mild thermal activation.
Single‐Site Active Cobalt‐Based Photocatalyst with a Long Carrier Lifetime for Spontaneous Overall Water Splitting
Authors: Liu Wei , Cao Linlin , Cheng Weiren , Cao Yuanjie , Liu Xiaokang , Zhang Wei , Mou Xiaoli , Jin Lili , Zheng Xusheng , Che Wei , Liu Qinghua , Yao Tao , Wei Shiqiang 
Published: May 2017

AbstractAn active and stable photocatalyst to directly split water is desirable for solar‐energy conversion. However, it is difficult to accomplish overall water splitting without sacrificial electron donors. Herein, we demonstrate a strategy via constructing a single site to simultaneously promote charge separation and catalytic activity for robust overall water splitting. A single Co1‐P4 site confined on g‐C3N4 nanosheets was prepared by a facile phosphidation method, and identified by electron microscopy and X‐ray absorption spectroscopy. This coordinatively unsaturated Co site can effectively suppress charge recombination and prolong carrier lifetime by about 20 times relative to pristine g‐C3N4, and boost water molecular adsorption and activation for oxygen evolution. This single‐site photocatalyst exhibits steady and high water splitting activity with H2 evolution rate up to 410.3 μmol h−1 g−1, and quantum efficiency as high as 2.2 % at 500 nm.
Partial crosslinking method for poly(2,6‐dimethyl‐1,4‐phenylene oxide)–poly(vinyl alcohol) membranes to obtain optimized stability and permeability
Authors: Yan Tingou , Zhu Hong , Gao Ke , Wu Cuiming , Wu Yonghui 
Published: May 2017

ABSTRACTA partial crosslinking method was developed to modify hydrophilic membranes. The membrane was sandwiched between two porous plates to protect part of the areas, then immersed into a crosslinking solution such as glutaraldehyde, and finally, set free from the plates. The protected and unprotected areas were alternatively distributed to form a heterogeneous membrane. The unprotected areas were crosslinked to enhance the membrane stability, whereas the protected areas retained their original permeability. Three types of hydrophilic base membranes were selected and prepared from poly(2,6‐dimethyl‐1,4‐phenylene oxide) and poly(vinyl alcohol). The base membranes were partially crosslinked (5.56% of the direct area with enlarged areas) to investigate their stability and diffusion dialysis (DD) performances. The partially crosslinked membranes had remarkably reduced water uptake and swelling degrees compared with the base membranes (72.4–250.4 vs 178.2%–544.4% and 94.0%–408.0% vs. 163.8%–814.8%). Meanwhile, the membranes still retained high DD performances for separating HCl–FeCl2 or NaOH–NaAlO2 solutions. The dialysis coefficients of HCl and NaOH were much higher than those of the fully crosslinked membranes (0.0209 vs. 0.0109 m/h and 0.0059–0.0085 vs. 0.0017–0.0022 m/h). Hence, partial crosslinking was effective in optimizing the membrane hydrophilicity and permeability. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45305.
Effects of relocation on metabolic profiles of freshwater mussels: Metabolomics as a tool for improving conservation techniques
Authors: Roznere Ieva , Watters G. Thomas , Wolfe Barbara A. , Daly Marymegan 
Published: May 2017

Abstract

Freshwater mussels are among the most endangered animals in North America. Although there are numerous state and federal programmes aimed at conserving them, knowledge of the effects of captivity and relocation remains limited.
Metabolomic techniques were used to assess the physiological state of freshwater mussels (Amblema plicata) relocated from the Muskingum River in Ohio to a conservation facility and to another stream in Ohio. Haemolymph samples were taken from mussels in the Muskingum River (MUS group), the facility (CAP group), and Big Darby Creek (DAR group) in September 2012 (month 3 post‐relocation), November 2012 (month 5), May 2013 (month 11), and August 2013 (month 14). Samples were analyzed by gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry.
In total, 95 biochemicals were identified during the 2012 sampling period and 104 biochemicals during the 2013 sampling period. Glucose and lipid metabolism remained similar among all groups. Differences between the MUS group and the CAP and DAR groups were observed in altered amino acid and nucleotide metabolism.
The results are indicative of a general stress response, which is evident for a year post‐relocation. Decreased levels of these metabolites are likely to be responsible for decreased growth rates and higher mortality often observed in relocated mussels.
Purification and functional characterization of lectin with phenoloxidase activity from the hemolymph of cockroach, Periplaneta americana
Authors: Arumugam Ganesh , Sreeramulu Bhuvaragavan , Paulchamy Ramaraj , Thangavel Sakthivel , Sundaram Janarthanan 
Published: May 2017

AbstractLectins also identified as hemagglutinins are multivalent proteins and on account of their fine sugar‐binding specificity play an important role in immune system of invertebrates. The present study was carried out on the hemolymph lectin of cockroach, Periplaneta americana with appropriate screening and purification to understand its molecular as well as functional nature. The lectin from the hemolymph was purified using ion‐exchange chromatography. The approximate molecular weight of purified lectin was 340
kDa as determined by FPLC analysis. Rabbit erythrocytes were highly agglutinated with purified lectin from the hemolymph of P. americana. The hemagglutination activity (HA) of lectin was specifically inhibited by fucose. Glycoproteins also inhibited the HA activity of lectin. The amino acid sequences of the purified lectin revealed homology with amino acid sequences of allergen proteins from P. americana. Purified lectin showed the highest phenoloxidase activity against dopamine. The activators such as exogenous proteases and LPS from Escherichia coli and Salmonella minnesota significantly enhanced the PO activity of the purified lectin. Besides, the presence of copper and hemocyanin conserved domain in the purified lectin provided a new facet that insects belonging to the ancient clade such as cockroaches retained some traces of evolutionary resemblance in possessing lectin of ancient origin.
Diketopyrrolopyrrole‐Porphyrin Based Conjugated Polymers for Ambipolar Field‐Effect Transistors
Authors: Zhou Shichao , Li Cheng , Zhang Jianqi , Yu Yaping , Zhang Andong , Wu Yonggang , Li Weiwei 
Published: May 2017

AbstractPorphyrin‐based molecules have been widely used in dye‐sensitized solar cells and bulk heterojunction solar cells, but their application in field‐effect transistors (FETs) is limited. In this work, two conjugated polymers based on diketopyrrolopyrrole and porphyrin units were developed for FETs. The polymers exhibit extra‐low band gap with energy levels close to −4.0 eV and −5.0 eV due to the strong electron‐donating and withdrawing ability of porphyrin and diketopyrrolopyrrole. With additionally high crystalline properties, ambipolar charge carrier transports with a hole mobility of 0.1 cm2 V−1 s−1 in FETs were realized in these polymers, representing the highest performance in solution‐processed FETs based on porphyrin unit.
The just world hypothesis as an argumentative resource in debates about unemployment benefits
Authors: Goodman Simon , Carr Philippa 
Published: May 2017

AbstractThe concept of the “just world” is established as a key explanation for how people make sense of inequality so that those deemed to score high in belief in a just world are more likely to hold prejudicial beliefs and to blame people in poverty for their situations. However, this is an inadequate explanation for such complicated and controversial issues. To better understand talk about the just world and the controversial issue of the distribution of unemployment benefits (an issue of inequality), a discursive psychological approach to the just world is used. Therefore, a discourse analysis focusses on 2 feature length British televised discussions about benefit claimants called “The Big Benefit Row: Live” (Channel 5 3/2/2014) and “Benefits Britain: the Debate” (Channel 4 17/2/2014). The analysis demonstrates that people draw on both just and unjust world arguments simultaneously and also topicalise what counts as just so as to support their positions on unemployment benefits, rather than in the consistent way that just world theory would predict. It is therefore argued that the just world should be recast as a cultural value that facilitates arguments about benefits, rather than an internally held belief.
A Fluorescent RNA Forced‐Intercalation Probe as a Pan‐Selective Marker for Influenza A Virus Infection
Authors: Haralampiev Ivan , Schade Matthias , Chamiolo Jasmine , Jolmes Fabian , Prisner Simon , Witkowski Peter T. , Behrent Marie , Hövelmann Felix , Wolff Thorsten , Seitz Oliver , Herrmann Andreas 
Published: May 2017

AbstractThe influenza A virus (IAV) genome is segmented into eight viral ribonucleoproteins, each expressing a negatively oriented viral RNA (vRNA). Along the infection cycle, highly abundant single‐stranded small viral RNAs (svRNA) are transcribed in a segment‐specific manner. The sequences of svRNAs and of the vRNA 5′‐ends are identical and highly conserved among all IAV strains. Here, we demonstrate that these sequences can be used as a target for a pan‐selective sensor of IAV infection. To this end, we used a complementary fluorescent forced‐intercalation RNA (IAV QB‐FIT) probe with a single locked nucleic acid substitution to increase brightness. We demonstrated by fluorescence in situ hybridization (FISH) that this probe is suitable and easy to use to detect infection of different cell types by a broad variety of avian, porcine, and human IAV strains, but not by other influenza virus types. IAV QB‐FIT also provides a useful tool to characterize different infection states of the host cell.
Descending aorta to right atrial fistula
Authors: Ho Andrew B. , Magee Alan G. , Hayes Nicholas 
Published: May 2017

AbstractHere, we report a four‐day old neonate presenting with cardiovascular collapse secondary to a descending aorta to right atrial fistula. Echocardiography was suggestive of the diagnosis and confirmed with computed tomographic (CT) imaging. The fistula was successfully occluded via cardiac catheterization using an Amplatzer Vascular Plug II implanted from the right atrial aspect. This report includes the first CT imaging of this extremely rare congenital defect as well as detailing the first successful transcatheter occlusion of this particular lesion.
SERS and SERRS Detection of the DNA Lesion 8‐Nitroguanine: A Self‐Labeling Modification
Authors: Dick Susan , Bell Steven E. J. , Alexander Katie J. , O'Neil Ian A. , Cosstick Richard 
Published: May 2017

AbstractRapid and sensitive methods to detect DNA lesions are essential in order to understand their role in carcinogenesis and for potential diagnosis of cancers. The 8‐nitroguanine DNA lesion, which is closely associated with inflammation‐induced cancers, has been characterized for the first time by surface‐enhanced Raman spectroscopy (SERS). This lesion has been studied as the free base, as well as part of a dinucleotide and oligodeoxynucleotides (ODNs) at 5 different excitation wavelengths in the range 785–488 nm. All nitrated samples produced distinctly different spectra from their control guanine counterparts, with nitro bands being assigned by DFT calculations. Additional resonance enhancement was observed at the shorter excitation wavelengths, these SERRS measurements allowed the detection of one nitrated guanine in over 1,300 bases. In addition, SER(R)S can be used to detect whether the unstable lesion is covalently attached to the ODN or has been released by hydrolytic depurination.
A DFT Study on the Stabilization of the B≡B Triple Bond in a Metallaborocycle: Contrasting Electronic Structures of Boron and Carbon Analogues
Authors: Ghorai Sagar , Jemmis Eluvathingal D. 
Published: May 2017

AbstractThe electronic structure of (η5‐Cp)2Zr(NH2‐BB‐NH2) (3 b) suggests that it could be a candidate for having a boron–boron triple bond in the cyclic system; however, computational studies shows that 3 b is a very high energy isomer on its potential energy surface. Replacement of amines with tricoordinate nucleophilic boron groups (η5‐Cp)2Zr[B(PH3)2‐BB‐B(PH3)2] (3 c) reduces the relative energy dramatically. The B≡B triple bond arises through the donation of two electrons from the metal fragment, ZrCp2, to the in‐plane π‐bonding orbital of the central B–B unit. Strong σ‐donating and chelating bis‐phosphine ligands (Me2P(CH2)nPMe2), which stabilize donor–acceptor bonding interaction in gem‐diborene L2B‐BBr2 (10), would be a good choice along the synthetic path towards 3 d, (η5‐Cp)2Zr[B4(Me2P(CH2)3PMe2)2]. A comparison of the energetics of the reaction leading to a cyclic boryne system (3 d), with the linear boryne isomer [(B2NHCPh)2] shows that the angle strain from cyclization is compensated by stabilization from the metal.