bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin A in total mixed ration silage influenced by various lactic acid bacteria: Lactobacillus plantarum, Lactobacillus casei and Enterococcus faecium
Authors: Cheng Yumeng , Wu Zidie , Bi Kaijian , Wang Xiqing , Yu Haizhong , Tian Pengjiao J. 
Published: October 2024

Abstract

This paper focuses on the impact of various strains of lactic acid bacteria (LAB) on vitamin A content in total mixed ration (TMR) silage. TMRs were respectively inoculated with Lactobacillus plantarum (LP), Lactobacillus casei (LC), and Enterococcus faecium (EF) and then ensiled for 56 days in the dark and anaerobic conditions. During ensiling, the fermentation quality, chemical composition, and microbial composition of TMR silage were analyzed, as well as the vitamin A. In addition, the free radical scavenging ability, total antioxidant capacity (T-AOC) and antioxidant enzyme activities (SOD, GSH-Px, CAT) of the three LAB strains were investigated. After 56 days of ensiling, the activities of T-AOC, SOD, GSH Px and CAT of TMR silage were also analysed. The results showed that LAB had significant effects on the fermentation quality and vitamin A of TMR silage (p<0.05). LP and LC could enhance the fermentation quality of TMR. Vitamin A loss rates in decreasing order were LC, CK, EF and LP groups. LAB endowed with elevated antioxidant properties are capable of augmenting the antioxidant capacity of total mixed ration (TMR) silage, thereby mitigating the degradation of vitamin A.
Assessment of Conservation Agricultural Practices on Soil Nutrient’s Stratification Ratio, Carbon Sequestration Rate, Management Indices and Crop Productivity in Southern Telangana India
Authors: Nthebere Knight , Tata Ram Prakash , Gudapati Jayasree , Bhimireddy Padmaja , Admala Meena , Chandran Latha P. , Yadav Manikyala Bhargava Narasimha 
Published: October 2024

Abstract

The impending crisis for food production is the biggest threat to sustenance of soil resources due to industrial farming practices adopted by multitudes of farmers on all parts of the world inclusive of the Southern Telangana Zone (STZ) in India. This can extensively degrade the soil if not substituted by soil resource-saving agricultural systems. This present experiment is implemented to assess the impact of contrasting tillage practices and weed control tactics on soil quality parameters (SQPs) and monitor the grain yield of maize after three-years in CA with a cotton-maize-Sesbania rostrata cropping system. Three tillage practices (main-plots); T1: CT(C)-CT(M)-fallow (NSr), T2: CT(C)-ZT(M)-ZT(Sr) and T3:ZT(C) + SrR-ZT(M) + CR-ZT(Sr) + MS and weed control tactics (sub-plots) involved; W1-chemical weed control, W2-Herbicide rotation, W3- Integrated weed management (IWM) and W4- single hand-weeded control in split-plot design. Sampling of the soil in the 0 − 15 and 15–30 cm, subsequent to harvesting of maize, was analyzed for pH, EC, soil macronutrient’s availability, soil organic carbon (SOC), and computed for stratification ratio (SR), C-sequestration rate (CSR), carbon management indices (CMI) and carbon retention efficiency (CRE) duly following the standard procedures. The salient findings indicated that 9.1%, 15.3% of SOC, 10.2%, 15.1% of available soil N, 12.2%, 19.6% of available soil P in the 0–15 cm and SR of 1.20 for SOC, 2.0–6.5% of active carbon (CACT) pool in the 0 − 30 cm was higher under T3 relative to T2, T1, respectively. Similarly, 36.0%, 58.1% of cumulative CSR, 29.4%, 58.8% of CRE in the 0 − 30 cm, and 17.0%, 30.3% of CMI in the 15–30 cm was higher T3 compared to T2, T1, respectively. The CPSV was the dominant contributor of SOC to total SOC over CACT in the 0–30 cm soil layer. The 49.0% and 52.0% of CACT pool were observed to be higher under T3 and single hand-weeded control, respectively. The T3 had higher Kernel yield (KY) of 8.4%, 11.6% in comparison with T2, T1, respectively. KY was also 23.4–43.1% greater under W1, W2, W3 over W4. The ZT with crop residue retention (T3), and IWM alternative to chemical weed control/ herbicide can be a viable solution to slow-down the soil degradation process and contribute towards enhanced crop productivity in cotton-maize-Sesbania rostrata cropping system in this zone.
Inequality and Gambling
Authors: Ferri Giovanni , Lagravinese Raffaele , Resce Giuliano 
Published: October 2024

Abstract

This study investigates the causal link between inequality and gambling on a distinct dataset that encompasses administrative records of gambling turnover by game type in Italian municipalities. Employing shift–share instruments, we find a substantial impact of inequality on gambling turnover. Moreover, we show that such impact is additional and larger than the impact of poverty and is biggest in less well-off Southern municipalities, especially concerning turnover generated from games closely associated with gambling-related issues. Given the documented regressive nature of gambling taxation, our results substantiate the concept of a hysteresis loop between inequality and gambling.This study investigates the causal link between inequality and gambling on a distinct dataset that encompasses administrative records of gambling turnover by game type in Italian municipalities. Employing shift–share instruments, we find a substantial impact of inequality on gambling turnover. Moreover, we show that such impact is additional and larger than the impact of poverty and is biggest in less well-off Southern municipalities, especially concerning turnover generated from games closely associated with gambling-related issues. Given the documented regressive nature of gambling taxation, our results substantiate the concept of a hysteresis loop between inequality and gambling.
JEL codes: I12; I14
Immunization with an adeno-associated viral vectored allergy vaccine containing Der p1-Der p2 effectively alleviates an asthmatic phenotype in mice
Authors: Chu Jiangzhou , Yin xiaolin , Xiong Anying , luo Yaoyao , Xin Jingxiu , Qin Shugang , He Zhongshan , Liu Shengbin 
Published: October 2024

Abstract

Given the rising incidence of allergic asthma, current symptomatic treatments primarily offer relief rather than halt disease progression. Recombinant allergens, designed with reduced immunoglobulin E (IgE) reactivity and the ability to regulate excessive T helper type 2 (Th2) responses, are emerging as promising candidates for more precise, effective, and safer specific immunotherapy (SIT). SIT remains the only clinical approach capable of potentially curing certain allergic diseases by inducing immunological tolerance. In this study, we explored the protective effects of AAV-Dp12S, an adeno-associated viral vector carrying two house dust mite antigens, Der p1 and Der p2, against allergic asthma. Using a murine model of HDM, immunization with this combination vaccine significantly attenuated the HDM-induced asthmatic phenotype. Invasive lung function assessments revealed improvements following AAV-Dp12S treatment, correlating with marked reductions in goblet cell hyperplasia and pulmonary eosinophilia. Moreover, total serum IgE, HDM-specific IgE (sIgE) titers, and pulmonary inducible nitric oxide synthase levels were effectively reduced. The cytokine profiles in bronchoalveolar lavage fluid (BALF) were modulated, as indicated by decreased levels of type 2 cytokines—interleukin (IL)-4, IL-5, and IL-13—and increased levels of interferon-γ (IFN-γ) and IL-10. Additionally, sIgE titers and production were significantly lowered. Overall, these findings demonstrate the potential of AAV-Dp12S as a therapeutic strategy for both tolerance induction and vaccination in the treatment of allergic asthma.
Contrastive Representation Learning withTransformers for Robust Auditory EEG Decoding
Authors: Bollens Lies , Accou Bernd , hamme Hugo Van , Francart Tom 
Published: October 2024

Abstract

Decoding of continuous speech from electroencephalography (EEG) presents a promising avenue for understanding neuralmechanisms of auditory processing and developing applications in hearing diagnostics. Recent advances in deep learning haveimproved decoding accuracy, but challenges remain due to the low speech-to-noise ratio of the recorded brain signals. Thisstudy explores the application of contrastive learning, a self-supervised learning technique, to learn robust latent representationsof EEG signals. We introduce a novel model architecture that leverages contrastive learning and transformer networks tocapture relationships between auditory stimuli and EEG responses. Our model is evaluated on two tasks from the ICASSP2023 Auditory EEG Decoding Challenge: match-mismatch classification and stimulus envelope regression. We achievestate-of-the-art performance on both tasks, significantly outperforming previous winners with 87% accuracy in match-mismatchclassification and a 0.176 Pearson correlation in envelope regression. Furthermore, we investigate the impact of modelarchitecture, training set size, and finetuning on decoding performance, providing insights into the factors influencing modelgeneralizability and accuracy. Our findings underscore the potential of contrastive learning for advancing the field of auditoryEEG decoding and its potential applications in clinical settings.
Pangenome analysis uncovers large hidden structural variants underlying fruit color and bacterial wilt resistance in eggplant
Authors: You Qian , Peng Ze , Li Zhiliang , Jiang Yaolan , Wan  Penglong , Zhao Yahui , Zhao Wei , Zhang Songyuan , Cheng Hefen , Chen Chengjie , Heng Zhou , Hu Ming , Zhou Yongfeng , Gaut Brandon , Sun Baojuan , Li Tao , Liao Yi 
Published: October 2024

Abstract

Large structural variants (SVs) underlying important agronomic traits remain largely unexplored in Solanaceae crops. Eggplant (Solanum melongena L.) is a globally important horticultural crop worldwide, belonging to the Solanaceae family. Here, we first present population genomic analyses of a diversity panel of 201 eggplant accessions, largely representing genetic diversity from one of the main eggplant domestication centers, Southeast Asia. We revealed that genetic relationships are highly correlated with geographic origin. We then de novo generated the chromosome-scale genomes of nine representative accessions, plus five previously published assemblies, to construct a graph-based pangenome. With the aid of this resource in association scans, we identified a 12.4 Mb inversion at a high frequency (53.7%) within the population, which is associated with fruit color, likely the result of artificial selection favoring purple peel. We also discovered multiple mutations linked to bacterial wilt resistance, including a premature stop codon in SmCYP82D47 and copy number variations of SmEPS1 and SmRoq1 homologs. This study provides insights into the evolution and biology of large SVs in Solanaceae crops, as well as provides fundamental genomic resources accessible to genetic analyses and breeding in eggplant.
Unveiling Novel Regulatory Mechanisms of BEX1 in Breast, Gastric, and Colorectal Cancer via a Systems Biology Approach: The Roles of lncRNAs COLCA1 and GAS6-AS1 and Their Interactions
Authors: Rezaei Mohammadreza , Salehipour Parnian , Tavakoli Mehrnoosh , Mousavi Maryam , Asgari Shima , Vatani Dorsan , Hosseinipouya Seyedeh Saba , Poudineh Younes , Rezaei Mohammad , Shirdeli Seyedeh Zahra , Ghelich Reza , Azadeh Mansoureh 
Published: October 2024

Abstract

Background: This study aimed to explore novel regulatory networks involving the BEX1 gene and its interaction with non-coding RNAs (ncRNAs) in breast cancer (BC), gastric cancer (GC), and colorectal cancer (CRC). BEX1 has been linked to tumor suppression, but its role in signaling pathways and its interactions with regulatory RNAs in these cancers has not been fully elucidated.
Methods: High-throughput microarray datasets (GSE10810, GSE54129, and GSE208099) were analyzed to investigate BEX1 expression in breast cancer, gastric cancer, and colorectal cancer. The expression analysis and survival outcomes for BEX1 and selected lncRNAs were validated using the ENCORI platform. Regulatory interactions of BEX1 with proteins and microRNAs were identified using STRING and miRWalk, respectively, while lncRNA interactions were examined through lncRRIsearch. Final validation of differential expression analysis and biomarker potential was conducted using qRT-PCR, along with ROC analysis to assess diagnostic capability.
Results: BEX1, identified as a tumor suppressor with low expression in breast, gastric, and colorectal cancer, demonstrated potential as a diagnostic biomarker, particularly in breast cancer (AUC: 0.8025, p = 0.0011). The lncRNAs COLCA1 and GAS6-AS1 were found to potentially regulate BEX1 expression. BEX1 exhibited significant interactions with two key proteins involved in cancer-related signaling pathways: CALML3 and LMO2. Moreover, BEX1 and these proteins demonstrated competitive interactions with miR-3616-3p, which was found to suppress BEX1 expression by targeting its 3'UTR. COLCA1 and GAS6-AS1 also exhibited dysregulated expression across breast, gastric, and colorectal cancers, suggesting their potential as diagnostic biomarkers.
Conclusion: The lncRNAs GAS6-AS1 and COLCA1, alongside miR-3616-3p, may play pivotal roles in regulating cancer-related pathways, including gastric acid secretion, insulin signaling, and homeostasis. These regulatory processes occur through direct and indirect interactions between the non-coding RNAs and BEX1, further highlighting the potential of these molecules as therapeutic targets and diagnostic biomarkers.
Exploring Waist-to-Hip Ratio and Health Risks: Insights from NFHS-5 on Trends, Patterns, and Predictors among the Indian Population
Authors: Gwalani Richa , Yadav Aarti , Shah Ragini , Pandya Apurva Kumar , Wadhwa Medha 
Published: October 2024

Abstract

Introduction: With a rising trend in obesity rates, particularly among young adults, understanding factors contributing to abdominal obesity is crucial for public health interventions. The study explores the significance of the Waist-to-Hip Ratio [WHR], focusing on the regional trends of abdominal obesity and associated factors.
Methods: The study made use of information from the National Family Health Survey 5, an extensive household survey which was conducted out in all of India's states and union territories.Socio-demographic variables such as age, gender, residence type, wealth index, and lifestyle factors were considered as independent variables. Descriptive statistics were used in statistical analysis along with chi-square tests, and logistic regression to identify predictors of increased WHR.
Results: Abdominal obesity, as indicated by WHR, was prevalent in 57.6% of the population, with 51.2% of males and 58.4% of females exhibiting high WHR values. Regional trends showed higher prevalence in the northern region of India. Significant correlations between WHR and variables such age, wealth index, type of dwelling, alcohol intake, and comorbid conditions were found by bivariate and regression analysis.Age and BMI were linked to increase WHR, and those with elevated blood pressure had a 1.24 times higher risk of increased WHR in contrast to people who have ideal Blood pressure.
Conclusion: The study emphasizes how critical it is to implement focused interventions in order to reduce India's rate of abdominal obesity. By comprehending the intricate relationship between lifestyle and socio demographic factors that impact WHR, public health initiatives can be customized to reduce the health hazards linked to central adiposity.
Identification of Disulfidptosis-Related LncRNA Subtypes, Establishment of a Prognostic Signature, and Characterization of Immune Infiltration in
Ovarian Cancer
Authors: Lin Jie , Liu Linying , Cai Xintong , Li Anyang , Fu Yixin , Huang Huaqing , Sun Yang 
Published: October 2024

Background:
Ovarian Cancer (OC) is a lethal malignant tumor with a poor prognosis.
Disulfidptosis is a newly identified form of cell death caused by disulfide stress. Targeting disulfidptosis
is a new metabolic therapeutic strategy in cancer treatment. We aimed to establish a disulfidptosis-
related lncRNA signature for prognosis prediction and explore its treatment values
in OC patients.

Method:
Data from the TCGA and GTEx databases and a disulfidptosis gene set were used to
establish a disulfidptosis-related lncRNA signature for prognosis prediction in OC patients.
Then, we internally and externally (PCR) validated our model. We also built a nomogram to improve
our model's predictive power. Afterward, GSEA was employed to explore our model's potential
functions. The ESTIMATE, CIBERSORT, TIMER, and ssGSEA were applied to estimate
the immune landscape. Finally, the drug sensitivity of certain drugs for OC patients was
analyzed.

Results:
We built a prognosis model based on seven drlncRNAs, including AL157871.2, HCP5,
AC027348.1, AL109615.3, AL928654.1, LINC02585, and AC011445.1. Our model performed
well by internal validation. PCR data also confirmed the same trend in the lncRNA levels. Furthermore,
the nomogram-integrated age, grade, stage, and risk score could accurately predict the
survival outcomes of OC patients. Subsequently, GSEA unveiled that our model genes enriched
the Hedgehog signaling pathway, a key regulator in OC tumorigenesis. Our predictive signature
was associated with immune checkpoints, such as PD-1(P < 0.01), PD-L1(P < 0.001), and
CTLA4 (P < 0.01), which might help screen out OC patients who are sensitive to immunotherapy.
Small molecule drugs, such as AZD-2281, GDC-0449, imatinib, and nilotinib, might benefit
OC patients with different risk scores.

Conclusion:
Our disulfidptosis-related lncRNA signature comprised of AL157871.2, HCP5,
AC027348.1, AL109615.3, AL928654.1, LINC02585, and AC011445.1 could serve as a prognostic
biomarker and guidance to therapy response for OC patients.
Bcr-Abl Inhibitory Activities of Imatinib Derivatives
Authors: Hou Bao-Long , Yan Jiaxu , Liu Rongrong , Lv Longquan , Feng Ruirui , Yang Xiumei , Guo Guanlin , Wang Cuiling 
Published: October 2024

Background:
Imatinib, a frontline targeted-therapeutic agent for patients with chronic
myelogenous leukemia (CML), was a synthetic tyrosine kinase inhibitor approved by the US Food
and Drug Administration.

Objective:
To expand the structural diversity of Bcr-Abl inhibitors, we synthesized nine novel
imatinib analogues (1a-1i) and evaluated their cytostatic effects against human cancer cell lines in
vitro.

Methods:
Imatinib and its analogues were successfully synthesized by an improved method in six
main steps. Inhibitory activities of all compounds were evaluated on K562, HL-60, MCF-7, A549
and PBMC in vitro. Then, the effect of the most active compound was studied using flow cytometer,
real-time qPCR and western blot experiments to determine its mechanism action. Finally, the molecular
docking of the most active compound were determined.

Results:
The IC50 of one imatinib analogue (compound 1h) for K562 and HL-60 was lower than
imatinib itself. Further studies indicate that the pro-apoptotic effect of compound 1h on K562 cells
was stronger than imatinib over a range of concentrations. Importantly, the real-time qPCR and
western blot experiments showed that compound 1h was superior to imatinib in inhibiting Bcr-Abl
expression. The structure-activity relationship was analyzed by determining the inhibitory rate of
each imatinib analogue. Introducing benzene (A ring) and piperidine (E ring) rings instead of pyridine
(A ring) and piperazine (E ring) in imatinib significantly enhanced the potency of imatinib
against K562 and HL-60 cell lines, which is consistent with the docking results.

Conclusion:
Imatinib analogue 1h showed a better inhibitory effect on leukemia cell lines than other
cell lines, which was consistent with the imatinib-like structure, moreover, had little effect on
PBMC. Overall, we conclude that compound 1h has the potential to treat chronic myeloid leukemia.
Nutritional Traits of Fatty Acid Composition of Common Carp Fed with Oil Sources
Authors: Delavariyan Raziyeh , Aberoumand Ali , Ziaei-nejad Saeed , Javaheri-baboli Mehran 
Published: October 2024

Background:
Cyprinus carpio, as a commercial fish in Iran, has an important effect on
human health and nutrition.

Aim:
The aim of this study was to investigate the effects of oil sources in the diet on the nutritional
value and fatty acid composition of C. carpio fish.

Methods:
Soybean oil (SO), palm oil (PO), fish oil (FO), and oil (soybean and palm) (SPO) contained
in the carp diet were fed.

Results:
The quality of fatty acids in the fish fillet from SO and PO was significantly better than
the FO diet. Total SFA (saturated fatty acids) in carp fillet fat fed with PO was significantly higher
than others (p<0.05). In carp fillets fed with experimental diets, there was a significant difference
between monounsaturated fatty acids, polyunsaturated fatty acids, high unsaturated fatty
acids, and n-3/n-6 fatty acids (p>0.05). The highest amounts of alpha-linolenic acid, linoleic acid,
and oleic acid were observed in fish fillets with FO and SO, respectively. Although the percentage
of eicosapentaenoic acid in fillets fed with FO and SPO diets was higher than in other treatments,
no significant difference in docosahexaenoic acid concentration was observed between all treatments
(p<0.05).

Conclusion:
The present study showed that oil sources (SO, PO, SPO) fed by C. carpio for 56
days are suitable in terms of productivity and efficiency.
Nanoencapsulation as an Ally of the Bioactive Compound Carvacrol: A Review of 10 Years of Advances
Authors: Romero-Castelán Ehira , Rodríguez-Hernández Adriana-Inés , Santos-López Eva-María , Cariño-Cortés Raquel , López-Ortega Mayra-Alejandra , Chavarría-Hernández Norberto , López-Cuellar Ma del Rocio 
Published: October 2024

Abstract:
Foodborne diseases (FBDs) are a major global public health problem, causing
millions of deaths annually and substantial economic losses. Antimicrobial treatment is
increasingly challenged by bacterial resistance. Essential oils from herbs and spices, such as
carvacrol from thyme and oregano, offer potential solutions due to their broad-spectrum
antimicrobial properties. However, its stability and its controlled release are affected by media
and environmental conditions. Nanoencapsulation presents a promising alternative to address
these challenges. This review analyzes 44 original papers and 21 patents concerning the recent
advancements in the nanoencapsulation of carvacrol over the past decade, focusing on natural
matrices and their applications in food, packaging, and human health fields. Various
encapsulation techniques and matrices have been explored, demonstrating that
nanoencapsulation can maintain the stability and antimicrobial efficacy of carvacrol. Moreover,
nanoencapsulated carvacrol shows promising applications in inhibiting biofilm formation and
quorum sensing, as well as exhibiting anticancer and anti-inflammatory effects. Patents related
to nanoencapsulated carvacrol highlight its potential for intelligent packaging and healthcare.
Nanoencapsulated carvacrol is a promising alternative to synthetic antimicrobials and as an
adjuvant in inflammatory disease treatments and cancer, offering enhanced efficacy and
versatility in applications.