bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Dietary Supplementation of Vitamin E, Moringa Oleifera Leaves and Carrots on growth performance and haemato-biochemical parameters of rabbit
Authors: Nazir Muhammad Muzammil , Hashmi Mohammad Rameez , Nazir Muhammad Mubashir , Ashraf Asma , Mahmood Nasir , suriya orooj , Abbasi Asim , Gulfreen Esha , Farzeen iqra 
Published: September 2024

Abstract

Rabbit meat has excellent nutritional and dietetic properties because it contains high protein contents, enriched with poly unsaturated fatty acids (PUFA), vitamin B complex and low cholesterol level which meet the requirements for modern consumer healthy lifestyle. Rabbit farming preferred over poultry farming due to low risk of diseases and no special care. The aim of this study is to evaluate the effect of dietary supplementation with vitamin E, Moringa oleifera leaves (MOFL) carrot on growth performance and haemato-biochemical parameters rabbit. A total of 60 rabbits with equal weights were randomly divided into four groups each contain 10 does and 5 buck and their dietary supplementation continued for 60 days. Animals were feed two times a day with basal diet. Group A (T1) kept as control, Group B treatment (T2) with 50mg/kg of carrot, Group C treatment (T3) with 50mg Vitamin E/kg B, and Group D (T4) 50gm/kg BW with Moringa oleifera leaves. Blood samples were collected from jugular vein at every 20-day interval and carried to laboratory for analysis. The result of the experiment showed that the group feed with MOL and Vitamin E improved parameter of hematology and lipid profile as well as growth parameters.
Cryoablation Combined with Programmed Cell Death Protein 1 Inhibitor Pembrolizumab for Advanced Non-small Cell Lung Cancer
Authors: Wang Li , Wang Guotai , Yuan Hubao , Cao Zhongqiang , Yang Xiaodong , Wang Pengli , Wang Shaohui 
Published: September 2024

Abstract

Introduction:
To evaluate the efficacy and safety of cryoablation combined with pembrolizumab treatment versus cryoablation alone in patients with advanced non-small cell lung cancer (NSCLC).
Methods
This retrospective study was conducted from February 2018 and October 2021. A total of 90 patients with NSCLC (AJCC stage IIIB/IV) were included, with 36 patients receiving cryoablation combined with pembrolizumab (Group A) and 54 patients receiving cryoablation alone (Group B). The primary outcome measures included objective response rate (ORR), overall survival (OS), and progression-free survival (PFS), immune responses and adverse events serving as secondary endpoints. Risk factors for OS and PFS were identified using univariate and multivariate analyses.
Results
No treatment related deaths were observed. Group A demonstrated a higher ORR (75.0% vs. 61.1%), longer median OS (28.1 months vs. 24.2 months), and longer median PFS (12.8 months vs. 8.4 months) compared to Group B. Additionally, Group A showed significant increases in CD3+, CD4+, and CD8 + T cells, and elevated levels of IL-2, IL-6, TNF-β, and IFN-γ. The multivariate analysis showed the combination of cryoablation and pembrolizumab was an independent prognostic factor for OS and PFS.
Conclusions: Cryoablation combined with pembrolizumab significantly improves clinical outcomes in advanced NSCLC patients compared to cryoablation alone, highlighting the potential of this combination therapy in enhancing anti-tumor immunity and prolonging survival.
An ERRα-ZEB1 transcriptional signature predicts survival in triple-negative breast cancers
Authors: Shi Jing-Ru , Poulard Coralie , Cerutti Catherine , Trédan Olivier , Romancer Muriel Le , Shi Tie-Liu , Vanacker Jean-Marc 
Published: September 2024

Abstract

Background.
Transcription factors (TFs) act together with co-regulators to modulate the expression of their target genes, which eventually dictates their pathophysiological effects. Depending on the co-regulator, TFs can exert different activities. The Estrogen Related Receptor α (ERRα) acts as a transcription factor that regulates several pathophysiological phenomena. In particular, interactions with PGC-1 co-activators are responsible for the metabolic activities of ERRα. In breast cancers, ERRα exerts several tumor-promoting, metabolism-unrelated activities that do not depend on PGC1, questioning the identity of the co-activators involved in these cancer-related effects.
Methods.
We used bio-computing methods to identify potential co-factors that could be responsible for the activities of ERRα in cancer progression. Experimental validations were conducted in different breast cancer cell lines, using determination of mRNA expression, ChIP-qPCR and proximity ligation assays.
Results.
ZEB1 is proposed as a major ERRα co-factor that could be responsible for the expression of direct ERRα targets in triple-negative breast cancers (TNBC). We establish that ERRα and ZEB1 interact together and are bound to the promoters of their target genes that they transcriptionally regulate. Our further analyses show that the ERRα-ZEB1 downstream signature can predict the survival of the TNBC patients.
Conclusions.
The ERRα-ZEB1 complex is a major actor in breast cancer progression and expression of its downstream transcriptional targets can predict the overall survival of triple-negative breast cancer patients.
Effect of Newborn Genomic Screening for Lysosomal Storage Disorders: A Randomized Clinical Trial in China
Authors: Wang Xin , Sun Yun , Guan Xian-Wei , Wang Yan-Yun , Hong Dong-Yang , Zhang Zhi-Lei , Li Ya-Hong , Yang Pei-Ying , jiang Tao , Xu Zheng-feng 
Published: September 2024

Abstract

Background
Lysosomal storage disorders (LSDs) have a relatively high incidence among rare diseases and can lead to severe consequences if not treated promptly. However, many countries and regions have not included these disorders in their newborn screening programs, resulting in missed early detection, underdiagnosis, and delayed treatment. Newborn genomic screening has shown good screening effectiveness for traditional biochemical screening diseases; however, its effectiveness for LSDs has not yet been evaluated in the general newborn population.
Methods
To evaluate the outcome of newborn genomic screening for LSDs, a cohort study was conducted involving newborns recruited from Nanjing Women and Children’s Healthcare Hospital in China from March 18, 2022, to September 21, 2023. All participants underwent genomic screening of 15 LSDs (18 genes) via dried blood spots, with enzyme activity testing of positive samples. The study calculated the prevalence and carrier rates for each LSD, as well as the positive screening rate, the false positive rate and the positive predictive value of the screening process.
Results
This study prospectively recruited 22,687 newborns (11,996 males [52.88%]). The mean (SD) gestational week was 39.2 (1.08) weeks, and the mean (SD) birth weight was 3369.7 (412.95) g. Genomic screening identified 1,344 carriers (6.0%) and 30 (0.13%) initial positive newborns for LSDs. Among the 30 initial positive newborns, 4 were excluded, 15 newborns were diagnosed, and 11 newborns were followed-up. The combined birth incidence of LSDs in Nanjing, Jiangsu Province, is 1/1,512, mainly Fabry disease, Krabbe disease, glycogen storage disease type II, Niemann-Pick disease and mucopolysaccharidosis type II. Compared with enzyme activity detection, newborn genomic screening had greater positive predictive value (P < 0.05) and a lower false positive rate (P < 0.05).
Conclusions
This study emphasizes the clinical utility of incorporating newborn genomic screening for LSDs into routine newborn screening, offering a proactive approach for early detection and intervention, ultimately enhancing public health and the well-being of newborns and their families.
Application of water management strategies in cooperative game theory for the restoration of Hour Al-Azim wetland
Authors: Pournabi Nima , Janatrostami Somaye , Ashrafzadeh Afshin , Mohammadi Kourosh 
Published: September 2024

Abstract

The Hour-Al-Azim transboundary wetland, one of Iran's most significant wetlands, is facing desiccation due to a disregard for environmental flow. A significant portion of the wetland has dried up, leading to the emergence of dust storms with detrimental effects on the region. Therefore, the imperative lies in fully restoring the wetland to mitigate the damages caused by dust storms and drought. In this study, considering the agricultural land area in the Karkheh River Basin and estimating the water requirements of the agricultural sector, the optimal allocation scenario that satisfies all stakeholders was determined using a cooperative game theory approach and Shapley value. The results indicated that complete wetland revival is not feasible considering the available water resources. Hence, solutions were proposed to reduce water consumption considering the virtual water trade approach and ecosystem services payment method. All potential options for the government and farmers toward complete wetland revival were identified using sequential game theory. Ultimately, the findings demonstrated that adopting a cooperative approach to reduce cultivated areas benefits both farmers by increasing profits and the government by simultaneously achieving the goal of wetland revival.
Multimodal assessment of brain stiffness variation in healthy subjects using magnetic resonance elastography and ultrasound time-harmonic elastography
Authors: Chandía Stefan Klemmer , Schattenfroh Jakob , Brinker Spencer T. , Tzschätzsch Heiko , Sack Ingolf , Meyer Tom 
Published: September 2024

Abstract

Magnetic resonance elastography (MRE) is a noninvasive method for mapping brain stiffness. Ultrasound-based transtemporal time-harmonic elastography (THE) is emerging as a cost-effective, fast alternative to MRE that has the potential to be used for bedside monitoring of intracranial pressure. We aim to investigate the accuracy of THE in direct comparison with MRE performed in the brain. Ten healthy volunteers (25–40 years old) successively underwent multifrequency MRE (20 Hz − 35 Hz) and THE (27 Hz − 56 Hz) of the brain. Fiducial-marker-based optical tracking of the ultrasound field of view was used to align THE to 3D MRE. THE- and MRE-derived shear wave speed (SWS) was determined as a measure of brain stiffness and averaged within different brain regions of various depths for cross-modality correlation analysis. MRE-measured SWS in temporal lobe regions ranged from 1.0 to 1.3 m/s and was negatively correlated with age (R2 = 0.44, p = 0.035). After registration of both modalities, there was linear correlation of SWS values (MRE: 1.14 ± 0.08 m/s, THE: 1.13 ± 0.10 m/s; R2 = 0.62, p = 0.007). Best agreement between MRE and THE was achieved at depths of 40 to 50 mm, suggesting this range provides a viable trade-off between ultrasound attenuation and near-field bias. Similar brain regions can be consistently measured with both elastography modalities, despite the regional and individual variations of stiffness, and provided that similar stimulation frequencies are used. Cost-effective and fast transtemporal THE yields stiffness values of the temporal lobe in a range similar to those obtained with more expensive MRE.
The sonic hedgehog signaling inhibitor cyclopamine improves pulmona ry arterial hypertension via regulating the bone morphogenetic protein receptor 2 pathway
Authors: Jin Youpeng , Mao Fei , Wang Xuehui , Zhang Jie , Gao Yanting , Fan Youfei 
Published: September 2024

Abstract

Pulmonary arterial hypertension (PAH) is a severe and progressive disease with hallmarks of pulmonary vascular remodeling and bone morphogenetic protein receptor 2 (BMPR2) mutation. Recent studies indicate Sonic hedgehog (SHH) signaling is involved in the proliferation of human pulmonary arterial smooth muscle cells (hPASMCs) but the role of the SHH signaling inhibitor cyclopamine in monocrotaline (MCT)-induced PAH has not been investigated. We hypothesized SHH promotes pulmonary vascular remodeling and that inhibition of SHH signaling by cyclopamine could attenuate pulmonary hypertension via the bone morphogenetic protein (BMP) pathway. SHH and BMPR2 proteins were measured in pulmonary arteries isolated from MCT-induced PAH rats and in hPASMCs. The therapeutic effects of cyclopamine were tested in PAH rats and in BMPR2 knockdown hPASMCs. SHH protein levels were increased in PAH rats and exogenous recombinant SHH protein promoted proliferation of hPASMCs via BMPR2 and osteopontin. Furthermore, cyclopamine attenuated hemodynamics and vascular remodeling via the BMP pathway in PAH rats. Finally, cyclopamine enhanced apoptosis and reduced proliferation in hPASMCs with impaired BMPR2. The findings of this study provide evidence that SHH has a role in pulmonary vascular remodeling via BMP4/BMPR2/ID1, and its inhibition by cyclopamine could be a potential therapeutic target in PAH.
Design and Numerical Simulation of CuBi2O4 SCs with GQDs as Hole Transport Layer: Ideal and Non-ideal Conditions
Authors: Panachikkool Muhammad , T Aparna E , Asaithambi Perumal , Pandiyarajan T. 
Published: September 2024

Abstract

The simulation of ideal and non-ideal conditions using the SCAPS-1D simulator for novel structure Ag/FTO/CuBi2O4/GQD/Au was done for the first time. The recombination of charge carriers in CuBi2O4 is an inherent problem due to very low hole mobility and polaron transport in the valence band. The in-depth analysis of the simulation result revealed that GQDs can act as an appropriate hole transport layer (HTL) and can enhance the hole transportation. The simulation was done under ideal and nonideal conditions. The non-ideal conditions include parasitic resistances, reflection losses, radiative, and Auger recombination whereas the ideal condition was studied without the inclusion of any losses. Under ideal conditions, the cell Ag/FTO/CuBi2O4/GQD/Au exhibited a photovoltaic (PV) parameter such as open circuit voltage (Voc), short circuit current (Jsc), fill factor (FF), photo conversion efficiency (PCE) are 1.39V, 25.898 mA/cm2, 90.92%, and 32.79%, respectively. The effect of various cell parameters such as the thickness of the absorber layer, HTL layer, and FTO, acceptor and defect density, the bandgap of the absorber and HTL layer, series and shunt resistance, back and front contact materials, radiation and Auger recombination of the absorber layer, reflection losses on the efficiency of the proposed cell is analysed. The drastic reduction in all PV parameters was observed under non-ideal conditions and the PV parameters are Voc (1.22V), Jsc (2.904 mA/cm2), FF (86.3), and PCE of 3.06%. The charge kinetics such as impedance, conductivity, and capacitance plots, and possible reasons for reductions in PV parameters are discussed in detail.
Evaluation of a novel simulation-based training for urgent laryngectomy care
Authors: Sparks Freya , Gilbody Nicky , Hilari Katerina 
Published: September 2024

Abstract

Background:
Laryngectomy (removal of the larynx, usually due to cancer) results in significant anatomical changes requiring specific clinical skills to safely manage the airway and support altered communication. It is crucial that healthcare professionals understand how to support people with laryngectomy, particularly in emergency care when their usual healthcare teams will not be present. Provision of laryngectomy training is limited. Existing education approaches fail to fully meet the needs of healthcare professionals; which in turn impacts on the provision of intervention to people with laryngectomy. With increasing evidence for simulation in pedagogical literature, this study explores how this approach can be used to support clinical skill education and improve urgent laryngectomy care. The aim of this study was to establish if a simulation-based approach is a feasible method of enhancing healthcare professional knowledge and confidence to provide emergency care to people with laryngectomy.
Methods:
A simulation-based training programme was piloted with delegates from a range of healthcare professions; over three separate study days. Immersive simulation scenarios were facilitated within a medical simulation centre using a modified SimMan mannequin, specially created models and prosthetics. Post-simulation debriefings were held with a focus on developing clinical skills within a Human Factors approach. In addition, training incorporated a skills-based session and interactive discussion with expert patients. Training was evaluated using pre- and post-course self-evaluation and qualitative feedback. Feasibility outcomes included the percentage of eligible participants who consented to take part, and the number of participants who completed the training.
Results:
28 multidisciplinary healthcare professionals registered for the training; 26 (93%) attended and completed the training activities as prescribed. Qualitative feedback and self-evaluation demonstrated increased theoretical knowledge and clinical skills post-training.
Conclusions:
Simulation-based training is a feasible method of clinical skill acquisition for urgent laryngectomy care. Further research is needed to assess whether competence is maintained over time, and whether Human Factors learning generalises to clinical practice. Wider study could incorporate assessment of the impact of the training on people with laryngectomy’s experiences of urgent care and potential impact on hospital flow.
Healthy longevity-associated protein improves cardiac function in murine models of cardiomyopathy with preserved ejection fraction
Authors: Alvino Valeria Vincenza , Slater Sadie , Qiu Yan , Cattaneo Monica , Gate Seamus , Sekar Vealmurugan , Puca Annibale , Madeddu Paolo 
Published: September 2024

Abstract

Aims: Aging is influenced by genetic determinants and comorbidities, among which diabetes increases the risk for heart failure with preserved ejection fraction. There is no therapy to prevent heart dysfunction in ageing and diabetic individuals. In previous studies, a single administration of the longevity-associated variant (LAV) of the human BPIFB4 gene halted heart decline in older and type-2 diabetic mice. Here, we asked whether orally administered LAV-BPIFB4 protein replicates these benefits.
Materials and Methods.In two controlled, randomized studies, 18-month-old male C57BL/6J mice and 9-week-old C57BLKS/J-Leprdb/Leprdb/Dock7+ [db/db] mice of both sexes underwent baseline echocardiography. They then received a recombinant purified LAV-BPIFB4 protein (3 µg/animal, every three days) or vehicle by gavage. After a 30-day, the animals underwent echocardiography and the hearts were collected post-termination for histology.
Results. All the animals completed the study except one female diabetic mouse, which was culled prematurely because of tooth malocclusion, which caused eating problems. There was no effect of the LAV-BPIFB4 protein on body weight in the two studies or glycosuria in the diabetic study. In aging mice, LAV-BPIFB4 increased myocardial BPIFB4 expression, improving heart contractility and capillarity while reducing perivascular fibrosis and senesce. In male diabetic mice, LAV-BPIFB4 therapy improved systolic function, microvascular density, and senescence, whereas the benefit was limited to systolic function in females.
Conclusions. This study shows the feasibility and efficacy of a variant protein associated with human longevity in contrasting pivotal risk factors for heart failure in animal models. The diabetic study revealed that sex influences the treatment efficacy.
ADHD medications: a descriptive study of prescription trends in Tabriz, Iran, 2021-2022
Authors: Fakhari Ali , Farahbakhsh Mostafa , Azimian Ali , Khameneh Amin , Matinkia Mahsa , Aghajani Ehsan 
Published: September 2024

Abstract

Introduction
Stimulants, which are also prescribed for other conditions, predominantly treat attention-deficit hyperactivity disorder (ADHD). Nevertheless, these medications are occasionally abused for nonmedical purposes. This study examines the demographic patterns in ADHD medication usage among both adult and pediatric populations in Tabriz, Iran, over a 12-month period.
Method
This descriptive study was conducted with data extracted from the Social Security Organization in Iran. Prescriptions were analyzed in Tabriz from March 2021 to March 2022. Prescription data were categorized by medication category, physician specialty, age group, and sex. Statistical analysis was subsequently performed via SPSS 2022.
Results
There were 21,083 prescriptions for ADHD drugs, which accounted for 1% of the target population. The average age of the recipients was 18.27 years, with males accounting for 66.8% and females accounting for 33.2%. The people who completed their medical education between 1987 and 1997 had the highest occurrence of ADHD medicine prescriptions. Psychiatrists accounted for the majority of prescriptions for ADHD medicines, accounting for 81.2% of prescriptions.
Conclusion
Only 1% of the target group received ADHD prescriptions. A lack of adequate ADHD diagnoses impedes many individuals from accessing crucial therapy. The mean age of the patients was 18.27 years, suggesting that older people might not be diagnosed as frequently as younger individuals. The most commonly prescribed medicine was methylphenidate, suggesting a reliance on a single treatment option. Specialists prescribed the majority of the medications. A scarcity of specialists may delay the diagnosis and treatment of ADHD. Providing education and training to primary care physicians can improve the quality of treatment received by patients.
BMC Health Services Research Regulation, modification, and evolution of remote sign language interpreting in Sweden – a work in progress
Authors: Warnicke Camilla , Matérne¹ Marie 
Published: September 2024

Abstract

Background
The sign language interpreting service has undergone a tremendous change due to COVID-19 as remote interpreting has become a more frequent alternative to the on-site face-to-face format. The aim of the study is to investigate the interpreters’ experience of the remote interpreting organisation in Sweden and how it evolved since the COVID-19 pandemic.
Method
Interviews with 26 experienced remote interpreters, representing 19 of Sweden’s 21 counties, were analysed with qualitative content analysis.
Results
Three themes were revealed in the analysis. The first theme was regulation. It was stated that directives and regulatory decisions concerning provision of remote interpreting services were varied and unclear. Several different platforms were used when interpreting remotely. Some of the services had conducted risk analyses, whereas others had not. The second theme was modification, including adjusting interactions to suit users’ preferences and capabilities, as well as adjustments to work environments and workplaces. The third theme, evolution of remote interpreting, showed that support and training were rare and varied. The organisation of remote interpreting appears to be a work in progress across Sweden, but it is a way of interpreting that is here to stay.
Conclusions
In Sweden, remote interpreting is a service that varies according to regulations and organisation of the assignments. The service seems to benefit from being more uniform and streamlined across Sweden, although consideration must be given to those involved with the service.