bluuebunny/crossref_metadata_2025_split huggingface dataset:
FOXQ1 regulates senescence-associated inflammation via activation of SIRT1 expression
Authors: Wang Pan , Lv Cuicui , Zhang Tao , Liu Junling , Yang Jin , Guan Fangxia , Hong Tianpei 
Published: July 2017

AbstractCellular senescence is an initial barrier to tumor development that prevents the proliferation of premalignant cells. However, some of the features of senescent cells seem to promote tumor progression via senescence-associated secretory phenotype (SASP). Here, we demonstrated that the protein level of forkhead box Q1 (FOXQ1), which highly overexpresses in several kinds of tumors, was significantly downregulated during both replicative and oncogene-induced senescence. Moreover, overexpression of FOXQ1 delayed senescence, whereas FOXQ1 silence led to premature senescence in human fibroblasts. Furthermore, we identified that FOXQ1 upregulated SIRT1 expression through transcriptional regulation via directly binding to the SIRT1 promoter. Finally, we showed that FOXQ1 remarkably inhibited the replicative senescence through depressing the expression of the inflammatory cytokines interleukin-6 (IL-6) and IL-8 via modulation of SIRT1-NF-κB pathway. In addition, FOXQ1 overexpressed in human esophageal cancer cells and ablation of FOXQ1 restrained the tumourigenic ability of the esophageal cancer cells (EC109 and EC9706) in a mouse xenograft model in vivo. Taken together, these findings uncover a previously unidentified role of FOXQ1 regulating SASP and tumor development at same time.
Different Potential of Extracellular Vesicles to Support Thrombin Generation: Contributions of Phosphatidylserine, Tissue Factor, and Cellular Origin
Authors: Tripisciano Carla , Weiss René , Eichhorn Tanja , Spittler Andreas , Heuser Thomas , Fischer Michael Bernhard , Weber Viktoria 
Published: July 2017

AbstractCells release diverse types of vesicles constitutively or in response to proliferation, injury, inflammation, or stress. Extracellular vesicles (EVs) are crucial in intercellular communication, and there is emerging evidence for their roles in inflammation, cancer, and thrombosis. We investigated the thrombogenicity of platelet-derived EVs, which constitute the majority of circulating EVs in human blood, and assessed the contributions of phosphatidylserine and tissue factor exposure on thrombin generation. Addition of platelet EVs to vesicle-free human plasma induced thrombin generation in a dose-dependent manner, which was efficiently inhibited by annexin V, but not by anti-tissue factor antibodies, indicating that it was primarily due to the exposure of phosphatidylserine on platelet EVs. Platelet EVs exhibited higher thrombogenicity than EVs from unstimulated monocytic THP-1 cells, but blockade of contact activation significantly reduced thrombin generation by platelet EVs. Stimulation of monocytic cells with lipopolysaccharide enhanced their thrombogenicity both in the presence and in the absence of contact activation, and thrombin generation was efficiently blocked by anti-tissue factor antibodies. Our study provides evidence that irrespective of their cellular origin, EVs support the propagation of coagulation via the exposure of phosphatidylserine, while the expression of functional tissue factor on EVs appears to be limited to pathological conditions.
Metabolic Biomarkers for Prognostic Prediction of Pre-diabetes: results from a longitudinal cohort study
Authors: Zeng Hailuan , Tong Renchao , Tong Wenxin , Yang Qiaoling , Qiu Miaoyan , Xiong Aizhen , Sun Siming , Ding Lili , Zhang Hongli , Yang Li , Tian Jingyan 
Published: July 2017

AbstractTo investigate the metabolic biomarkers of predicting the transition from pre-diabetes (pre-DM) to normal glucose regulation (NGR) and diabetes (DM) in a longitudinal cohort study. 108 participants with pre-DM were followed up for ten years and divided into 3 groups according to different glycemic outcomes. 20 participants progressed to DM, 20 regressed to NGR, and 68 remained at pre-DM. Alterations in plasma metabolites in these groups were evaluated by untargeted ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Twenty three metabolites related to glycerophospholipid metabolism, oxidation and antioxidation were associated with the process from pre-DM to NGR, while twenty two metabolites related to amino acid metabolism, glycerophospholipid metabolism and mitochondrial β-oxidation played important roles in the progression to DM. Results from stepwise logistic regression analysis showed that five biomarkers (20-Hydroxy-leukotriene E4, Lysopc(20:4), 5-methoxytryptamine, Endomorphin-1, Lysopc(20:3)) were good prediction for the restoration to NGR, and five biomarkers (Iso-valeraldehyde, linoleic acid, Lysopc(18:1), 2-Pyrroloylglycine, Dityrosine) for the development of DM. The findings suggest that the combination of these potential metabolites may be used for the prognosis of pre-DM. Targeting the pathways that involved in these prognostic biomarkers would be beneficial for the regression to NGR and the early prevention of DM among pre-DM.
Evidence linking microRNA suppression of essential prosurvival genes with hippocampal cell death after traumatic brain injury
Authors: Boone Deborah Kennedy , Weisz Harris A. , Bi Min , Falduto Michael T. , Torres Karen E. O. , Willey Hannah E. , Volsko Christina M. , Kumar Anjali M. , Micci Maria-Adelaide , Dewitt Douglas S. , Prough Donald S. , Hellmich Helen L. 
Published: July 2017

AbstractThe underlying molecular mechanisms of how dysregulated microRNAs (miRNAs) cause neurodegeneration after traumatic brain injury (TBI) remain elusive. Here we analyzed the biological roles of approximately 600 genes - we previously found these dysregulated in dying and surviving rat hippocampal neurons - that are targeted by ten TBI-altered miRNAs. Bioinformatic analysis suggests that neurodegeneration results from a global miRNA-mediated suppression of genes essential for maintaining proteostasis; many are hub genes - involved in RNA processing, cytoskeletal metabolism, intracellular trafficking, cell cycle progression, repair/maintenance, bioenergetics and cell-cell signaling - whose disrupted expression is linked to human disease. Notably, dysregulation of these essential genes would significantly impair synaptic function and functional brain connectivity. In surviving neurons, upregulated miRNA target genes are co-regulated members of prosurvival pathways associated with cellular regeneration, neural plasticity, and development. This study captures the diversity of miRNA-regulated genes that may be essential for cell repair and survival responses after TBI.
The Association of Ambient Temperature and Violent Crime
Authors: Tiihonen Jari , Halonen Pirjo , Tiihonen Laura , Kautiainen Hannu , Storvik Markus , Callaway James 
Published: July 2017

AbstractIt is controversial if global warming will result into increased crime and conflict rate, and no causal neurobiological mechanisms have been proposed for the putative association between ambient temperature and aggressive behavior. This study shows that during 1996–2013, ambient temperature explained 10% of variance in the violent crime rate in Finland, corresponding to a 1.7% increase/degree centigrade. Ambient temperature also correlated with a one month delay in circannual changes in peripheral serotonin transporter density among both offenders and healthy control subjects, which itself correlated strongly with the monthly violent crime rate. This suggests that rise in temperature modulates serotonergic transmission which may increase impulsivity and general human activity level, resulting into increase in social interaction and risk of violent incidents. Together, these results suggest that the effect of ambient temperature on occurrence of violent crime is partly mediated through the serotonergic system, and that a 2 °C increase in average temperatures would increase violent crime rates by more than 3% in non-tropical and non-subtropical areas, if other contributing factors remained constant.
The Epigenetic Landscape of Promoter Genome-wide Analysis in Breast Cancer
Authors: Karsli-Ceppioglu Seher , Dagdemir Aslihan , Judes Gaëlle , Lebert André , Penault-Llorca Frédérique , Bignon Yves-Jean , Bernard-Gallon Dominique 
Published: July 2017

AbstractBreast cancer is a heterogeneous disease due to its clinico-pathological features and response to therapy. The classification of breast tumors based on their hormone receptor status and pathologic features. Post-translational histone modifications come into prominence for regulation of gene expression in cancer pathogenesis. Here, we analyzed dysregulation of H3K9ac and H3K27me3-enriched subtype-specific genes using ChIP-on-chip assay in breast cancer tumors and matched normal tissue samples. Breast cancer tumors were classified according to St Gallen Consensus 2013. Our results indicated that the promoter regions of genes modified by H3K9ac epi-mark are commonly associated with tumors with HER2-positive and TNBC subtype. H3K27me3-enriched genes were comprised of Luminal A and B1 subtypes. We constructed a network structure to elicit epigenetically regulated genes related with breast cancer progression. The central genes of the network (RUNX1, PAX3, GATA4 and DLX5) were subjected for epigenetically dysregulation in association with different breast cancer subtypes. Our study submits epigenetic mechanisms are crucial to elicit subtype-specific regulation in breast cancer and ChIP-on-chip assay provides a better understanding for breast tumorigenesis and new approaches for prevention and treatment.
Dietary patterns reflecting healthy food choices are associated with lower serum LPS activity
Authors: Ahola Aila J , Lassenius Mariann I. , Forsblom Carol , Harjutsalo Valma , Lehto Markku , Groop Per-Henrik 
Published: July 2017

AbstractGram-negative bacteria-derived lipopolysaccharides (LPS) are associated with various negative health effects. Whether diet is associated with LPS, is an understudied phenomenon. We investigated the association between diet and serum LPS activity in 668 individuals with type 1 diabetes in the FinnDiane Study. Serum LPS activity was determined using the Limulus Amoebocyte Lysate assay. Diet was assessed with a food frequency questionnaire (FFQ) section of a diet questionnaire and a food record. The food record was used to calculate energy, macronutrient, and fibre intake. In a multivariable model, energy, macronutrient, or fibre intake was not associated with the LPS activity. Using factor analysis, we identified seven dietary patterns from the FFQ data (“Sweet”, “Cheese”, “Fish”, “Healthy snack”, “Vegetable”, “Traditional”, and “Modern”). In a multivariable model, higher factor scores of the Fish, Healthy snack, and Modern patterns predicted lower LPS activity. The validity of the diet questionnaire was also investigated. The questionnaire showed reasonable relative validity against a 6-day food record. The two methods classified participants into the dietary patterns better than expected by chance. In conclusion, healthy dietary choices, such as consumption of fish, fresh vegetables, and fruits and berries may be associated with positive health outcomes by reducing systemic endotoxaemia.
The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
Authors: Li Quanlin , Li Wenbo , Li Xingzheng , Liu Lulu , Zhang Ying , Guo Yuying , Chen Xia , Xu Guiyun , Zheng Jiangxia 
Published: July 2017

AbstractThe major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells’ viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.
Involvement of the leptin-adiponectin axis in inflammation and oxidative stress in the metabolic syndrome
Authors: Frühbeck Gema , Catalán Victoria , Rodríguez Amaia , Ramírez Beatriz , Becerril Sara , Salvador Javier , Portincasa Piero , Colina Inmaculada , Gómez-Ambrosi Javier 
Published: July 2017

AbstractThe aim of the present work was to study whether the leptin-adiponectin axis may have a pathophysiological role in the increased systemic inflammation and oxidative stress observed in patients with the metabolic syndrome (MS). Leptin, adiponectin, and markers of inflammation and oxidative stress were measured in a sample of 140 Caucasian subjects (74 males/66 females), aged 28–82 years, 60 with and 80 without the MS. Total concentrations of adiponectin as well as its multimeric forms HMW, MMW and LMW were significantly lower in individuals with the MS. The ratio adiponectin/leptin, a marker of dysfunctional adipose tissue, was dramatically decreased in the MS group. Systemic oxidative stress, as evidenced by levels of thiobarbituric acid reactive substances (TBARS), as well as markers of inflammation such as serum amyloid A (SAA), C-reactive protein (CRP) and osteopontin were significantly increased in subjects with the MS. Total adiponectin concentrations were negatively correlated with levels of TBARS and CRP levels. Furthermore, the ratio adiponectin/leptin was negatively correlated with SAA concentrations as well as with CRP levels. We concluded that a dysfunctional adipose tissue as suggested by a low adiponectin/leptin ratio may contribute to the increased oxidative stress and inflammation, hallmarks of the MS.
Burn-induced muscle metabolic derangements and mitochondrial dysfunction are associated with activation of HIF-1α and mTORC1: Role of protein farnesylation
Authors: Nakazawa Harumasa , Ikeda Kazuhiro , Shinozaki Shohei , Kobayashi Masayuki , Ikegami Yuichi , Fu Ming , Nakamura Tomoyuki , Yasuhara Shingo , Yu Yong-Ming , Martyn J. A. Jeevendra , Tompkins Ronald G. , Shimokado Kentaro , Yorozu Tomoko , Ito Hideki , Inoue Satoshi , Kaneki Masao 
Published: July 2017

AbstractMetabolic derangements are a clinically significant complication of major trauma (e.g., burn injury) and include various aspects of metabolism, such as insulin resistance, muscle wasting, mitochondrial dysfunction and hyperlactatemia. Nonetheless, the molecular pathogenesis and the relation between these diverse metabolic alterations are poorly understood. We have previously shown that burn increases farnesyltransferase (FTase) expression and protein farnesylation and that FTase inhibitor (FTI) prevents burn-induced hyperlactatemia, insulin resistance, and increased proteolysis in mouse skeletal muscle. In this study, we found that burn injury activated mTORC1 and hypoxia-inducible factor (HIF)-1α, which paralleled dysfunction, morphological alterations (i.e., enlargement, partial loss of cristae structure) and impairment of respiratory supercomplex assembly of the mitochondria, and ER stress. FTI reversed or ameliorated all of these alterations in burned mice. These findings indicate that these burn-induced changes, which encompass various aspects of metabolism, may be linked to one another and require protein farnesylation. Our results provide evidence of involvement of the mTORC1-HIF-1α pathway in burn-induced metabolic derangements. Our study identifies protein farnesylation as a potential hub of the signaling network affecting multiple aspects of metabolic alterations after burn injury and as a novel potential molecular target to improve the clinical outcome of severely burned patients.
Holistic assessment of call centre performance
Authors: Smith Edward A. , Schormans John A. 
Published: July 2017

In modern call centres 60–70% of the operational costs come in the form of the human agents who take the calls. Ensuring that the call centre operates at lowest cost and maximum efficiency involves a trade‐off of the cost of agents against lost revenue and increased customer dissatisfaction due to lost calls. Modelling the performance characteristics of a call centre in terms of the agent queue alone misses key performance influencers, specifically the interaction between channel availability at the media gateway and the time a call is queued. A blocking probability at the media gateway, as low as 0.45%, has a significant impact on the degree of queuing observed and therefore the cost and performance of the call centre.Our analysis also shows how abandonment impacts queuing delay. However, the call centre manager has less control over this than the level of contention at the media gateway. Our commercial assessment provides an evaluation of the balance between abandonment and contention, and shows that the difference in cost between the best and worst strategy is £130K per annum, however this must be balanced against a possible additional £2.98 m exposure in lost calls if abandonment alone is used.
Recent Progress on the Synthesis of (Aza)indoles through
Oxidative Alkyne Annulation Reactions
Authors: Xu Hai-Chao , Hou Zhong-Wei , Mao Zhong-Yi 
Published: July 2017

The oxidative [3+2] cycloaddition of alkynes with arylamines is a powerful method for the synthesis of (aza)indoles because it employs unfunctionalized and easily available materials. Herein, recent progress in the synthesis of (aza)indoles through transition metal-catalyzed oxidative [3+2] cycloaddition is highlighted.1	Introduction2	Second-Row Transition-Metal Catalysts3	First-Row Transition-Metal Catalysts4	Summary
Revision Anterior Cruciate Ligament Reconstruction with Contralateral Hamstring Tendon Grafts: 6 Years Follow-Up
Authors: Legnani Claudio , Zini Stefania , Borgo Enrico , Ventura Alberto 
Published: July 2017

Purpose The purpose of this study was to retrospectively evaluate the clinical outcome of revision anterior cruciate ligament (ACL) reconstruction with contralateral hamstring tendon autografts, specifically with regard to patient satisfaction, return to preinjury activity level, and postoperative functional outcomes.
Methods Between 2004 and 2011, 23 patients underwent revision ACL reconstruction with contralateral autogenous hamstring tendon grafts and were retrospectively reviewed at an average follow-up of 6.3 years. Subjective and functional evaluations were performed. The Tegner score, Knee Injury and Osteoarthritis Outcome Score (KOOS), and International Knee Documentation Committee (IKDC) Subjective Knee Form were used. Objective evaluation included range of motion, Lachman test, pivot-shift test, and KT-1000 instrumented laxity testing. Wilcoxon test was used to compare the preoperative and follow-up status. Differences with a p-value of <0.05 were considered statistically significant.
Results No major complications were reported. The mean KOOS significantly increased from a preoperative mean of 62.8 ± 8.3 to 85.8 ± 6.9 (p < 0.001). IKDC subjective score significantly improved from 29.2 ± 10.4 to 72.8 ± 5.2 (p < 0.001). The median Tegner activity score significantly improved from a preoperative mean of 6.5 (range: 4–10) to 7.5 (range: 7–10) (p < 0.001). Most of the patients increased or returned to the same activity level, with 61% of the patients returning to cutting and pivoting sports.
Conclusion The use of contralateral hamstring tendon autografts for ACL revision surgery represents a valid option following a failed primary ACL reconstruction and confirms subjective and objective clinical improvement 6 years after surgery.
Level of Evidence Level IV, therapeutic case series.
Advanced Computed Tomography Techniques: Overview of Dual-Energy CT
Authors: Forghani Reza 
Published: July 2017

AbstractDual-energy computed tomography (DECT) is an advanced form of computed tomography (CT), in which image acquisition is performed at two different energy spectra, instead of a single-energy spectrum using conventional single-energy CT (SECT). This enables the creation of different reconstructions and quantitative spectral tissue analysis beyond what is possible with SECT. In adults, there are increasing clinical applications of DECT for all organ systems, including neuroimaging and head and neck imaging. However, there are relatively few studies evaluating applications of DECT for pediatric imaging and little to none in neuroimaging or head and neck imaging. The purpose of this article is to provide an overview and familiarize the readers with DECT. This article will review the fundamental principles behind DECT, including different DECT acquisition systems and principles of DECT material characterization. This will be followed by a review of potential applications of DECT, many based on imaging the head and neck. The objectives are to familiarize the readers with this exciting technology and hopefully serve as a primer for investigations and applications of DECT for pediatric neuro and head and neck imaging.
Ernährung bei multimorbiden Patienten – Daten oder Meinungen?
Authors: Plauth Mathias 
Published: July 2017

AbstractPolymorbidity and old age are rather the rule than the exception in hospitalised patients. Malnutrition is common in such patients and should be identified by appropriate screening and assessment measures in order to devise a nutrition plan and act accordingly. Unlike in the UK or The Netherlands, malnutrition screening and nutrition teams are not mandatory for German hospitals. Malnutrition and, in particular, sarcopenia are indicators of a nutrition associated risk or increased morbidity and mortality. Malnutrition can affect patients of any medical discipline and, therefore, is managed most efficiently by the interdisciplinary and multiprofessional nutrition team. By this approach goal directed nutrition therapy can improve morbidity and mortality of hospitalised patients.
Weibliches urethrales Divertikel mit Steinbildung imitierend eine Belastungsinkontinenz
Authors: Karapanos Leonidas , Porres Daniel , Pfister David , Akbarov Ilgar , Heidenreich Axel , Zugor Vahudin 
Published: July 2017

ZusammenfassungDivertikel in der Urethra von Frauen (DU) sind ein seltenes Krankheitsbild mit einer Prävalenz von 0,6 – 4,7 % 1. Ätiologisch liegen häufig eine chronische Entzündung der paraurethralen Drüsen, ein Geburtstrauma oder eine iatrogene Läsion nach Urethramanipulationen zugrunde. Selten liegen angeborene Fälle vor. Wir präsentieren einen Fall eines weiblichen UD mit Steinbildung, das im Rahmen einer Inkontinenzabklärung diagnostiziert wurde. Wir möchten anhand dieser Kasuistik die bestehende Literatur bezüglich Symptomatik, Diagnostik und Therapie dieses seltenen Krankheitsbildes darstellen. Schließlich möchten wir betonen, dass in der Differentialdiagnose einer Belastungsinkontinenz der klinische Ausschluss eines UD unerlässlich ist. Dafür werden genaue Kenntnisse der Symptomatik und des diagnostischen Algorithmus zur Detektion eines DU benötigt.