bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hand‐Ground Nanoscale ZnII‐Based Coordination Polymers Derived from NSAIDs: Cell Migration Inhibition of Human Breast Cancer Cells
Authors: Paul Mithun , Sarkar Koushik , Deb Jolly , Dastidar Parthasarathi 
Published: February 2017

AbstractIncreased levels of intracellular prostaglandin E2 (PGE2) have been linked with the unregulated cancer cell migration that often leads to metastasis. Non‐steroidal anti‐inflammatory drugs (NSAIDs) are known inhibitors of cyclooxygenase (COX) enzymes, which are responsible for the increased PGE2 concentration in inflamed as well as cancer cells. Here, we demonstrate that NSAID‐derived ZnII‐based coordination polymers are able to inhibit cell migration of human breast cancer cells. Various NSAIDs were anchored to a series of 1D ZnII coordination polymers through carboxylate–Zn coordination, and these structures were fully characterized by single‐crystal X‐ray diffraction. Hand grinding in a pestle and mortar resulted in the first reported example of nanoscale coordination polymers that were suitable for biological studies. Two such hand‐ground nanoscale coordination polymers NCP1 a and NCP2 a, which contained naproxen (a well‐studied NSAID), were successfully internalized by the human breast cancer cells MDA‐MB‐231, as was evident from cellular imaging by using a fluorescence microscope. They were able to kill the cancer cells (MTT assay) more efficiently than the corresponding mother drug naproxen, and most importantly, they significantly inhibited cancer cell migration thereby displaying anticancer activity.
Pulmonary adenocarcinoma with mucin production modulates phenotype according to common genetic traits: a reappraisal of mucinous adenocarcinoma and colloid adenocarcinoma
Authors: Sonzogni Angelica , Bianchi Fabrizio , Fabbri Alessandra , Cossa Mara , Rossi Giulio , Cavazza Alberto , Tamborini Elena , Perrone Federica , Busico Adele , Capone Iolanda , Picciani Benedetta , Valeri Barbara , Pastorino Ugo , Pelosi Giuseppe 
Published: February 2017

AbstractWhether invasive mucinous adenocarcinoma (IMA) and colloid adenocarcinoma (ICA) of the lung represent separate tumour entities, or simply lie within a spectrum of phenotypic variability, is worth investigating. Fifteen ICA, 12 IMA, 9 ALK‐rearranged adenocarcinomas (ALKA), 8 non‐mucinous KRAS‐mutated adenocarcinomas (KRASA) and 9 mucinous breast adenocarcinomas (MBA) were assessed by immunohistochemistry for alveolar (TTF1, cytoplasmic MUC1), intestinal (CDX‐2, MUC2), gastric (membrane MUC1, MUC6), bronchial (MUC5AC), mesenchymal (vimentin), neuroendocrine (chromogranin A, synaptophysin), sex steroid hormone‐related (oestrogen and progesterone receptors), pan‐mucinous (HNF4A) and pan‐epithelial (keratin 7) lineage biomarkers and by targeted next generation sequencing (TNGS) for 50 recurrently altered cancer genes. Unsupervised clustering analysis using molecular features identified cluster 1 (IMA and ICA), cluster 2 (ALKA and KRASA) and cluster 3 (MBA) (p < 0.0001). Cluster 1 showed four histology‐independent sub‐clusters (S1 to S4) pooled by HFN4A and MUC5AC but diversely reacting for TTF1, MUC1, MUC2, MUC6 and CDX2. Sub‐cluster S1 predominantly featured intestinal‐alveolar, S2 gastrointestinal, S3 gastric and S4 alveolar differentiation. In turn, KRASA and ALKA shared alveolar lineage alongside residual MUC5AC expression, with additional focal CDX2 and diffuse vimentin, respectively. A proximal‐to‐distal scheme extending from terminal (TB) and respiratory (RB) bronchioles to alveolar cells was devised, where S3 originated from distal TB (cellular mucinous adenocarcinoma), S2 from proximal RB (secreting mucinous adenocarcinoma), S1 from intermediate RB (mucin lake‐forming colloid adenocarcinoma), S4 from distal RB (colloid alveolar adenocarcinoma), KRASA from juxta‐alveolar RB (KRAS‐mutated non‐mucinous adenocarcinoma) and ALKA from juxta‐bronchial alveolar cells (ALK‐translocated adenocarcinoma). TNGS analysis showed KRAS, LKB1, TP53, APC and CDKN2A mutation predominance. In conclusion, IMA and ICA are basket categories, which likely originate from distinct domains of stem/progenitor cells spatially distributed along bronchioles upon common molecular features and genetic alterations.
Honeycomb‐like Nitrogen and Sulfur Dual‐Doped Hierarchical Porous Biomass‐Derived Carbon for Lithium–Sulfur Batteries
Authors: Chen Manfang , Jiang Shouxin , Huang Cheng , Wang Xianyou , Cai Siyu , Xiang Kaixiong , Zhang Yapeng , Xue Jiaxi 
Published: February 2017

AbstractHoneycomb‐like nitrogen and sulfur dual‐doped hierarchical porous biomass‐derived carbon/sulfur composites (NSHPC/S) are successfully fabricated for high energy density lithium–sulfur batteries. The effects of nitrogen, sulfur dual‐doping on the structures and properties of the NSHPC/S composites are investigated in detail by transmission electron microscopy (TEM), X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), and charge/discharge tests. The results show that N, S dual‐doping not only introduces strong chemical adsorption and provides more active sites but also significantly enhances the electronic conductivity and hydrophilic properties of hierarchical porous biomass‐derived carbon, thereby significantly enhancing the utilization of sulfur and immobilizing the notorious polysulfide shuttle effect. Especially, the as‐synthesized NSHPC‐7/S exhibits high initial discharge capacity of 1204 mA h g−1 at 1.0 C and large reversible capacity of 952 mA h g−1 after 300 cycles at 0.5 C with an ultralow capacity fading rate of 0.08 % per cycle even at high sulfur content (85 wt %) and high active material areal mass loading (2.8 mg cm−2) for the application of high energy density Li–S batteries.
A method for characterizing phenotypic changes in highly variable cell populations and its application to high content screening of Arabidopsis thaliana protoplasts
Authors: Johnson Gregory R. , Kangas Joshua D. , Dovzhenko Alexander , Trojok Rüdiger , Voigt Karsten , Majarian Timothy D. , Palme Klaus , Murphy Robert F. 
Published: March 2017

AbstractQuantitative image analysis procedures are necessary for the automated discovery of effects of drug treatment in large collections of fluorescent micrographs. When compared to their mammalian counterparts, the effects of drug conditions on protein localization in plant species are poorly understood and underexplored. To investigate this relationship, we generated a large collection of images of single plant cells after various drug treatments. For this, protoplasts were isolated from six transgenic lines of A. thaliana expressing fluorescently tagged proteins. Eight drugs at three concentrations were applied to protoplast cultures followed by automated image acquisition. For image analysis, we developed a cell segmentation protocol for detecting drug effects using a Hough transform‐based region of interest detector and a novel cross‐channel texture feature descriptor. In order to determine treatment effects, we summarized differences between treated and untreated experiments with an L1 Cramér‐von Mises statistic. The distribution of these statistics across all pairs of treated and untreated replicates was compared to the variation within control replicates to determine the statistical significance of observed effects. Using this pipeline, we report the dose dependent drug effects in the first high‐content Arabidopsis thaliana drug screen of its kind. These results can function as a baseline for comparison to other protein organization modeling approaches in plant cells. © 2017 International Society for Advancement of Cytometry
Mechano‐sensitive regulation of gene expression during the embryonic development
Authors: Eroshkin Fedor M. , Zaraisky Andrey G. 
Published: February 2017

AbstractCell movements during embryogenesis produce mechanical tensions that shape the embryo and can also regulate gene expression, thereby affecting cell differentiation. Increasing evidence indicates that mechanosensitive regulation of gene expression plays important roles during embryogenesis by coupling the processes of morphogenesis and differentiation. However, the molecular mechanisms of this phenomenon remain poorly understood. This review focuses on the molecular mechanisms that “translate” mechanical stimuli into gene expression.
Genomic array as compared to karyotyping in myelodysplastic syndromes in a prospective clinical trial
Authors: Stevens‐Kroef Marian J. , Olde Weghuis Daniel , ElIdrissi‐Zaynoun Najat , van der Reijden Bert , Cremers Eline M. P. , Alhan Canan , Westers Theresia M. , Visser‐Wisselaar Heleen A. , Chitu Dana A. , Cunha Sonia M. , Vellenga Edo , Klein Saskia K. , Wijermans Pierre , de Greef Georgine E. , Schaafsma M. Ron , Muus Petra , Ossenkoppele Gert J. , van de Loosdrecht Arjan A. , Jansen Joop H. 
Published: February 2017

AbstractKaryotyping is considered as the gold standard in the genetic subclassification of myelodysplastic syndrome (MDS). Oligo/SNP‐based genomic array profiling is a high‐resolution tool that also enables genome wide analysis. We compared karyotyping with oligo/SNP‐based array profiling in 104 MDS patients from the HOVON‐89 study. Oligo/SNP‐array identified all cytogenetically defined genomic lesions, except for subclones in two cases and balanced translocations in three cases. Conversely, oligo/SNP‐based genomic array profiling had a higher success rate, showing 55 abnormal cases, while an abnormal karyotype was found in only 35 patients. In nine patients whose karyotyping was unsuccessful because of insufficient metaphases or failure, oligo/SNP‐based array analysis was successful. Based on cytogenetic visible abnormalities as identified by oligo/SNP‐based genomic array prognostic scores based on IPSS/‐R were assigned. These prognostic scores were identical to the IPSS/‐R scores as obtained with karyotyping in 95%‐96% of the patients. In addition to the detection of cytogenetically defined lesions, oligo/SNP‐based genomic profiling identified focal copy number abnormalities or regions of copy neutral loss of heterozygosity that were out of the scope of karyotyping and fluorescence in situ hybridization. Of interest, in 26 patients we demonstrated such cytogenetic invisible abnormalities. These abnormalities often involved regions that are recurrently affected in hematological malignancies, and may therefore be of clinical relevance. Our findings indicate that oligo/SNP‐based genomic array can be used to identify the vast majority of recurrent cytogenetic abnormalities in MDS. Furthermore, oligo/SNP‐based array profiling yields additional genetic abnormalities that may be of clinical importance.
Multi‐phase decompression modeling of CO2 pipelines
Authors: Liu Bin , Liu Xiong , Lu Cheng , Godbole Ajit , Michal Guillaume , Tieu Anh Kiet 
Published: February 2017

AbstractCarbon capture and storage (CCS) is a technology that has been proposed to reduce what are perceived to be excessive concentrations of carbon dioxide (CO2) in the atmosphere. CCS will require the transportation of CO2 from the capture locations to the storage sites via pipelines. To ensure safety, an accurate prediction of CO2 decompression following pipeline fracture is crucial for the design and operation for these projects. A multi‐phase CO2 pipeline decompression model using computational fluid dynamics (CFD) techniques is presented in this paper. The GERG‐2008 equation of state (EOS) is employed to describe the properties of vaporand liquid phases. A phase change model using a mass transfer coefficient to control the inter‐phase mass transfer rateis implemented into the CFD code. By varying the mass transfer coefficient, the effect of delayed nucleation on the decompression wave speed can be investigated. The proposed multi‐phase CFD decompression modelis validated against the experimental data from a shock tube test. The performance of the proposed model is also compared with that of the Homogeneous Equilibrium Model (HEM). In addition, the influence of delayed nucleation on CO2 decompression characteristics is discussed and an optimum mass transfer coefficient for CO2 depressurization is obtained. © 2017 Society of Chemical Industry and John Wiley & Sons, Ltd.
Treatment‐related determinants of survival in early‐stage (T1–2N0M0) oral cavity cancer: A population‐based study
Authors: Sowder Justin C. , Cannon Richard B. , Buchmann Luke O. , Hunt Jason P. , Hitchcock Ying , Lloyd Shane , Grossmann Kenneth F. , Monroe Marcus M. 
Published: February 2017

ABSTRACTBackgroundNational guidelines support both surgical and radiotherapy (RT) as initial treatment options for early‐stage oral cavity squamous cell carcinoma (SCC). There remains limited data evaluating the survival outcomes of RT and the current practice patterns for these lesions.MethodsWe conducted a retrospective review of 8274 patients in the Surveillance, Epidemiology, and End Results (SEER) database from 1988 to 2008 with T1 to T2N0M0 oral cavity SCC. Primary outcomes were 5‐year overall survival (OS) and disease‐specific survival (DSS).ResultsSurgical therapy had significantly improved OS (140 months; p < .001) and DSS (217 months; p < .001) compared to surgery with adjuvant RT (104 and 163 months, respectively) and definitive RT (68 and 136 months, respectively). The use of radiation alone was associated with an increased T classification, hard palate, retromolar trigone primary site lesions, and advanced patient age.ConclusionPrimary radiation without surgery continues to be used in a subset of early‐stage oral cavity SCCs, in which it is associated with decreased OS and DSS. © 2017 Wiley Periodicals, Inc. Head Neck 39: 876–880, 2017
Positron emission tomography–CT prediction of occult nodal metastasis in recurrent laryngeal cancer
Authors: Rosko Andrew , Birkeland Andrew , Shuman Andrew , Prince Mark , Bradford Carol , Wolf Gregory , Worden Francis , Eisbruch Avraham , Srinivasan Ashok , Wong Ka Kit , Spector Matthew E. 
Published: February 2017

ABSTRACTBackgroundThe purpose of this study was to evaluate the predictive value of positron emission tomography (PET)‐CT in identifying occult nodal metastasis in clinically and radiographically N0 patients with recurrent laryngeal cancer undergoing salvage laryngectomy.MethodsRetrospective review of 46 clinically and radiographically N0 patients with recurrent laryngeal cancer who underwent a PET‐CT examination before salvage laryngectomy with neck dissection from January 1, 2002, to December 31, 2014, was performed.ResultsTwo patients (16.7%) had true‐positive PET‐CT results, whereas 10 patients (83.3%) had false‐negative scans, 1 patient (2.9%) had a false‐positive result and 33 patients (97.1%) had a true‐negative PET‐CT. The sensitivity of PET‐CT was 16.7% (95% confidence interval [CI], 3.5% to 46.0%) with a specificity of 97.1% (95% CI, 83.8% to 99.9%), positive predictive value (PPV) of 66.7% (95% CI, 20.2% to 94.4%), and negative predictive value (NPV) of 76.7% (95% CI, 62.1% to 87.0%).ConclusionPET‐CT has poor sensitivity and NPV making PET‐CT an imperfect predictor of nodal disease in recurrent laryngeal cancer. © 2017 Wiley Periodicals, Inc. Head Neck 39: 980–987, 2017
Correlating the depth of invasion at specific anatomic locations with the risk for regional metastatic disease to lymph nodes in the neck for oral squamous cell carcinoma
Authors: Brockhoff Hans C. , Kim Roderick Y. , Braun Thomas M. , Skouteris Christos , Helman Joseph I. , Ward Brent B. 
Published: February 2017

ABSTRACTBackgroundThe purpose of this study was to investigate the critical primary tumor depth of invasion in oral squamous cell carcinoma that would lead to a 20% or greater risk of nodal metastasis.MethodsAn institutional review board approved retrospective review of our head and neck database was performed from 2009 to 2014 and the data were statistically analyzed.ResultsTwo hundred eighty‐six patients with a diagnosis of oral squamous cell carcinoma who met our inclusion criteria underwent primary excision and neck dissection. For a depth of invasion of 1 mm or less, there were no patients with a positive node. From 1.1 mm to 2 mm of depth of invasion, there was 1 of 11 patients (9%) who had at least 1 positive node. At 2.1 mm to 3 mm, 5 of 25 patients (20%) had at least 1 positive node.ConclusionDepth of invasion and the location of the tumor are 2 important variables to consider when making treatment recommendations to patients with clinical N0 disease. © 2017 Wiley Periodicals, Inc. Head Neck 39: 974–979, 2017
Integration of high‐risk human papillomavirus into cellular cancer‐related genes in head and neck cancer cell lines
Authors: Walline Heather M. , Goudsmit Christine M. , McHugh Jonathan B. , Tang Alice L. , Owen John H. , Teh Bin T. , McKean Erin , Glover Thomas W. , Graham Martin P. , Prince Mark E. , Chepeha Douglas B. , Chinn Steven B. , Ferris Robert L. , Gollin Susanne M. , Hoffmann Thomas K. , Bier Henning , Brakenhoff Ruud , Bradford Carol R. , Carey Thomas E. ,   The University of Michigan Head and Neck Specialized Program of Research Excellence (SPORE) Program
Published: February 2017

ABSTRACTBackgroundHuman papillomavirus (HPV)‐positive oropharyngeal cancer is generally associated with excellent response to therapy, but some HPV‐positive tumors progress despite aggressive therapy. The purpose of this study was to evaluate viral oncogene expression and viral integration sites in HPV16‐ and HPV18‐positive squamous cell carcinoma lines.MethodsE6/E7 alternate transcripts were assessed by reverse transcriptase‐polymerase chain reaction (RT‐PCR). Detection of integrated papillomavirus sequences (DIPS‐PCR) and sequencing identified viral insertion sites and affected host genes. Cellular gene expression was assessed across viral integration sites.ResultsAll HPV‐positive cell lines expressed alternate HPVE6/E7 splicing indicative of active viral oncogenesis. HPV integration occurred within cancer‐related genes TP63, DCC, JAK1, TERT, ATR, ETV6, PGR, PTPRN2, and TMEM237 in 8 head and neck squamous cell carcinoma (HNSCC) lines but UM‐SCC‐105 and UM‐GCC‐1 had only intergenic integration.ConclusionHPV integration into cancer‐related genes occurred in 7 of 9 HPV‐positive cell lines and of these 6 were from tumors that progressed. HPV integration into cancer‐related genes may be a secondary carcinogenic driver in HPV‐driven tumors. © 2017 Wiley Periodicals, Inc. Head Neck 39: 840–852, 2017
Retrospective analysis of placenta previa with abnormal placentation with and without prophylactic use of abdominal aorta balloon occlusion
Authors: Cui Shihong , Zhi Yunxiao , Cheng Guomei , Zhang Kai , Zhang Lindong , Shen Linna 
Published: February 2017

AbstractObjectiveTo evaluate the effectiveness of prophylactic abdominal aorta balloon occlusion in cases of placenta previa with abnormal placentation.MethodsIn a retrospective study, data were analyzed for patients who had placenta previa with placenta accreta and underwent elective cesarean delivery (>34 weeks) with or without temporary aortic balloon occlusion at a center in Zhengzhou, China, between October 2015 and September 2016. The primary clinical outcomes were operative time, estimated blood loss, intraoperative blood transfusion volume, hemoglobin, hysterectomy, and hospitalization.ResultsAmong 69 eligible women, 38 had temporary balloon occlusion, and 31 had no balloon occlusion. Operative time, blood transfusion volume, change in hemoglobin, hysterectomy, and length of hospitalization did not differ. Although mean blood loss did not differ, fewer patients in the balloon group than the non‐balloon group had an estimated blood loss of more than 1000 mL (24 [63%] vs 28 [90%]; P=0.009). In terms of different placental types, the estimated blood loss among women with placenta accreta and placenta increta was lower in the balloon group than in the non‐balloon group (P<0.001 and P=0.01, respectively).ConclusionProphylactic balloon occlusion of the abdominal aorta reduced bleeding after cesarean delivery for women with abnormal placentation.
Evaluation of Infiltration Models and Variability of Soil Infiltration Properties at Multiple Scales
Authors: Nie Weibo , Ma Xiaoyi , Fei Liangjun 
Published: March 2017

AbstractSoil infiltration is characterized by scale dependence, so the accuracy and reliability of indirect methods used to estimate soil infiltration properties from available soil physical data are often below expectations. This study aimed to determine the scale‐dependent relationship between soil infiltration and soil physical properties and to establish pedotransfer functions (PTFs) for estimating normalization factors (FC). Field studies were conducted using double‐ring infiltrometers in the first (sandy loam) and third (clay loam) terraces in Yangling District. Multiple scale variability and relationships were studied by multifractal and joint multifractal techniques. Results indicated that the Kostiakov–Lewis model best described the relationship between cumulative infiltration and time in the study areas, and that the normalization equation for predicting cumulative infiltration was accurate. Joint multifractal analysis showed that the variability of FC in the first terrace was related largely to sand content, soil bulk density, and silt content. FC in the third terrace depended on the contents of sand, silt, initial soil water, and clay at multiple scales. The PTFs that were established based on the results of joint multifractal analysis reliably estimated FC values. Copyright © 2017 John Wiley & Sons, Ltd.
Analytical evaluation of the ADAMS™ A1c HA8180T analyzer for the measurement of HbA1c
Authors: Urrechaga Eloísa 
Published: February 2017

BackgroundThe ADAMS™ A1c HA‐8180T (ARKRAY, Inc., Kyoto, Japan) is a high pressure liquid chromatography system designed for separation and quantitation of HbA1c, while detecting hemoglobin (Hb) variants and HbA2, with an analysis time of just 3.5 min. The aim was to evaluate the analytical performance of this analyzer for routine HbA1c quantitation.MethodsTrueness, imprecision, carry over, linearity, the effect of anemia and correlation with ADAMS™ A1c HA‐8180V (in Variant mode) were assessed according to guidelines of the Clinical and Laboratory Standards Institute (CLSI) and manufacturer. The presence of coexisting interfering chemically modified Hb variants was also assessed.ResultsThe results were found to be accurate (total error of 1.74%) and precise: within‐run, between‐run, between‐day and total coefficients of variation were 0.71%, 0.77%, 0.36%, and 0.93% at low concentrations, and 0.43%, 0.29%, 0%, and 0.48% at high concentrations of HbA1c. Carry over, linearity, and correlation of methods were excellent. HbA1c measurement was independent of total Hb concentration in a range 40‐214 g/L, and shows no interference from Hb chemically modified commonly observed in this type of analysis, while common Hb variants are detected.ConclusionThe analyzer is a suitable system for glycemic control in patients with diabetes and the detection of Hb variants.