bluuebunny/crossref_metadata_2025_split huggingface dataset:
PP2A binds to the LIM domains of lipoma-preferred partner through its PR130/B″ subunit to regulate cell adhesion and migration
Authors: Janssens Veerle , Zwaenepoel Karen , Rossé Carine , Petit Marleen M. R. , Goris Jozef , Parker Peter J. 
Published: March 2016

ABSTRACT
Here, we identify the LIM protein lipoma-preferred partner (LPP) as a binding partner of a specific protein phosphatase 2A (PP2A) heterotrimer that is characterised by the regulatory PR130/B″α1 subunit (encoded by PPP2R3A). The PR130 subunit interacts with the LIM domains of LPP through a conserved Zn2+-finger-like motif in the differentially spliced N-terminus of PR130. Isolated LPP-associated PP2A complexes are catalytically active. PR130 colocalises with LPP at multiple locations within cells, including focal contacts, but is specifically excluded from mature focal adhesions, where LPP is still present. An LPP–PR130 fusion protein only localises to focal adhesions upon deletion of the domain of PR130 that binds to the PP2A catalytic subunit (PP2A/C), suggesting that PR130–LPP complex formation is dynamic and that permanent recruitment of PP2A activity might be unfavourable for focal adhesion maturation. Accordingly, siRNA-mediated knockdown of PR130 increases adhesion of HT1080 fibrosarcoma cells onto collagen I and decreases their migration in scratch wound and Transwell assays. Complex formation with LPP is mandatory for these PR130-PP2A functions, as neither phenotype can be rescued by re-expression of a PR130 mutant that no longer binds to LPP. Our data highlight the importance of specific, locally recruited PP2A complexes in cell adhesion and migration dynamics.
Test-Retest Reliability of Physiological and Performance Responses to 120 Minutes of Simulated Soccer Match Play
Authors: Harper Liam D. , Hunter Robert , Parker Paul , Goodall Stuart , Thomas Kevin , Howatson Glyn , West Daniel J. , Stevenson Emma , Russell Mark 
Published: March 2016

Abstract
Harper, LD, Hunter, R, Parker, P, Goodall, S, Thomas, K, Howatson, G, West, DJ, Stevenson, E, and Russell, M. Test-retest reliability of physiological and performance responses to 120 minutes of simulated soccer match play. J Strength Cond Res 30(11): 3178–3186, 2016—This study investigated the test-retest reliability of physiological and performance responses to 120 minutes (90 minutes plus 30 minutes extra-time [ET]) of the soccer match simulation (SMS). Ten university-standard soccer players completed the SMS on 2 occasions under standardized conditions. Capillary and venous blood was taken pre-exercise, at half-time, and at 90 and 120 minutes, with further capillary samples taken every 15 minutes throughout the exercise. Core temperature (T
core
), physical (20- and 15-m sprint speeds and countermovement jump height), and technical (soccer dribbling) performance was also assessed during each trial. All variables except blood lactate demonstrated no systematic bias between trials (p > 0.05). During the last 15 minutes of ET, test-rest reliability (coefficient of variation %, Pearson's r, respectively) was moderate to strong for 20-m sprint speed (3.5%, 0.71), countermovement jump height (4.9%, 0.90), dribble speed (2.8%, 0.90), and blood glucose (7.1%, 0.93), and very strong for T
core
(1.2%, 0.99). Moderate reliability was demonstrated for 15-m sprint speed (4.6%, 0.36), dribble precision (11.5%, 0.30), plasma insulin (10.3%, 0.96), creatine kinase ([CK] 28.1%, 0.38), interleukin-6 (24%, 0.99), nonesterified fatty acids ([NEFA] 13.2%, 0.73), glycerol (12.5%, 0.86), and blood lactate (18.6%, 0.79). In the last 15 minutes of ET, concentrations of blood glucose and lactate and sprint and jump performances were reduced, whereas T
core
, NEFA, glycerol, and CK concentrations were elevated (p ≤ 0.05). The SMS is a reliable protocol for measuring responses across the full 120 minutes of soccer-specific exercise. Deleterious effects on performance and physiological responses occur during ET.
Effects of Angle Variations in Suspension Push-up Exercise
Authors: Gulmez Irfan 
Published: March 2016

Abstract
Gulmez, I. Effects of angle variations in suspension push-up exercise. J Strength Cond Res 31(4): 1017–1023, 2017—This study aimed to determine and compare the amount of loads on the TRX Suspension Trainer (TRX) straps and ground reaction forces at 4 different angles during TRX push-ups. Twenty-eight male (mean age, 24.1 ± 2.9 years; height, 179.4 ± 8.0 m; weight, 78.8 ± 9.8 kg) physical education and sports university students participated in this study. The subjects were tested at TRX angles (0, 15, 30, 45°) during the TRX push-ups. Force data were recorded by a force platform and load cells integrated into the TRX straps. The results show that as the TRX angle was reduced, the load applied to the TRX straps increased and simultaneously the load measured by the force platform decreased. This was true for both the elbow joint changing from flexion to extension and vice versa. When the TRX angle was set at 0° and subjects' elbows were at extension during TRX push-up, 50.4% of the subjects' body weight, and when the elbows were at flexion, 75.3% of the body weight was registered by the sensors on the TRX straps. The results of this study can be used in the calculation of the training load and volume (resistance training programming) during TRX push-up exercises at varying angles.
The Kansas Squat Test Modality Comparison: Free Weights vs. Smith Machine
Authors: Luebbers Paul E. , Fry Andrew C. 
Published: March 2016

Abstract
Luebbers, PE and Fry, AC. The Kansas squat test modality comparison: free weights vs. smith machine. J Strength Cond Res 30(8): 2186–2193, 2016—Standardized methods of testing power are instrumental in planning and implementing training regimens for many athletes, and also in tracking training adaptations. Previous work has demonstrated that the Kansas squat test (KST) is a valid test for measuring indices of mean and peak power when compared with the Wingate anaerobic cycle test. Although the KST was designed for use with a Smith machine (SM), many power athletes use free weights for training. The purpose of this study was to determine the feasibility of using free weights (FW) for the KST by comparing it with the SM modality. Twenty-three track and field athletes participated (mean ± SD; weight, 69.7 ± 10.6 kg; age, 20.1 ± 1.1 years) in this study. Each completed familiarization sessions with the FW and SM modalities before data collection. A 1-repetition maximum squat was also determined for both the FW and SM. Correlation coefficients indicated significant relationships between the FW KST and SM KST on measures of peak test power (r = 0.955; p < 0.01) and mean test power (r = 0.959; p < 0.01) but not for relative fatigue (r = −0.198; p > 0.05) or posttest lactate (r = 0.109; p > 0.05). Paired samples t-tests indicated that the FW KST resulted in significantly higher measures of peak power and mean power (p ≤ 0.01), although no differences were observed for relative fatigue or lactate (p > 0.05). These data indicate that the FW KST is a valid and feasible alternative to the SM KST in measuring peak and mean power.
Characterization of Microchannel Hemodialyzers Using Residence Time Distribution Analysis
Authors: Coblyn Matthew , Truszkowska Agnieszka , Jovanovic Goran 
Published: March 2016

Abstract
Microchannel-based hemodialysis has a potential to improve survival rates and quality of life for end-stage renal disease patients compared to conventional hemodialysis technology. Characterization of hydrodynamic behavior in microchannel geometries is necessary for improving flow uniformity, a critical challenge in realizing a commercial device. A test loop was developed for measuring the impulse response of a tracer dye injected into a dialyzer test article for the purpose of developing residence time distributions (RTD) to characterize lamina design. RTD variance tended to lower for designs that are more dominated, volume-wise, by the microchannel array versus the headers. RTD results also emphasize how defect issues can significantly impact a microchannel device via discrepancies between conceptual and operational devices. A multisegmented CFD model, developed for pairing with the impulse response test loop and dialyzer, showed good agreement between visual observation of the tracer in simulations and experiments, and the shape and peak of the output profiles.
First report of <i>Monoeca</i> in Argentina, with description of two new species (Hymenoptera: Apidae)
Authors: Torretta Juan Pablo , Roig-Alsina Arturo 
Published: March 2016

Two new species of the oil-collecting bee genus Monoeca Lepeletier & Audinet-Serville (Apidae: Tapinotaspidini) are described and figured from females and males captured in northeastern Argentina. The two new species here described, Monoeca armata Torretta & Roig-Alsina, new species, and M. puchella Torretta & Roig-Alsina, new species, are easily distinguished from each other by the color of the integument, the wings, and the pilosity. Their relationships to already described species of the genus are discussed. Monoeca were the only genus of Tapinostaspidini not known to occur in Argentina. The flower records and the study of the pollen scopal loads corroborate the association of these oil-collecting bees with plants of the family Malpighiaceae.
O consumo do sexo em Copacabana/RJ e a conformação de suas territorialidades: embates e resistências
Authors: Bassetti Telma Bittencourt , Peixoto Roberta 
Published: March 2016

No presente artigo, apresentamos o espaço urbano como condição, produto e meio de toda a atividade humana que se realiza no cotidiano. O território, por sua vez, é definido a partir de seus usos e múltiplas relações de identidade. O objeto de pesquisa é o consumo do corpo mediado pelo dinheiro no bairro de Copacabana na cidade do Rio de Janeiro, cujo desdobramento se dá na conformação de territórios voltados para o consumo do corpo enquanto atividade de lazer. Esses territórios são famosos entre os turistas que conhecem esse espaço como “territórios do prazer”. Ao mesmo tempo, e contraditoriamente, o bairro é também residencial e detém o maior número absoluto de idosos entre os bairros do país. Assim, o principal objetivo é investigar a relação entre a população residente e a população que está ali para o consumo do sexo no que diz respeito aos seus embates e resistências.
Prevalencia de síndrome de burnout en alumnos del séptimo año de medicina de una universidad privada de Lima, Perú.
Authors: Estela-Villa Luis M. , Jiménez-Román Carlos R. , Landeo-Gutiérrez Jeremy S. , Tomateo-Torvisco Jorge David , Vega-Dienstmaier Johann Martín 
Published: March 2016

El síndrome de burnout (SBO) se ha descrito como una entidad patológica común entre los médicos y que podríaoriginarse durante su formación académica. Objetivo: Determinar la prevalencia y los factores asociados al SBO enalumnos de medicina del séptimo año de la Universidad Peruana Cayetano Heredia (UPCH). Material y Métodos: Serealizó un estudio transversal con los estudiantes de medicina del séptimo año de la UPCH (n=117) durante diciembre del 2010. Se empleó dos encuestas autoadministradas anónimas, una sobre características sociodemográficas y laborales y otra, la escala Maslach Burnout Inventory en su versión Human Services Survey validada en español. Resultados: Se encontró una prevalencia de SBO de 57,2% (n=59). Más de la mitad de la población presentó niveles medio o alto en despersonalización (DP) (68,9% [n=71]) y agotamiento emocional (AE) (67% [n =69]) y aproximadamente un tercio de los estudiantes tuvieron baja realización personal (RP) (35% [n=36]). No se encontró asociación estadísticamente significativa entre las variables sociodemográficas y laborales estudiadas y la presenciade SBO. La presencia de enfermedad mental se asoció a niveles altos de DP y AE, mientras la ausencia de actividadesextracurriculares se asoció a elevados puntajes en AE. Conclusiones: El SBO estuvo presente en más de la mitad delos internos de medicina de la UPCH. La presencia de enfermedad mental y ausencia de actividades extracurriculares se asociaron a las dimensiones del SBO. (Rev Neuropsiquiatr 2010;73:147-156).
Seguín y la psicoterapia: Aspectos de su vida y obra
Authors: Peña K. Saúl 
Published: March 2016

Este trabajo es expresión de mi gratitud hacia mi maestro, profesor Carlos Alberto Seguín, auténtico precursor delpsicoanálisis en el Perú, así como de nuestra relación significativa y trascendente, su Weltanschauung sigue viva yestimula mi interioridad en una mutualidad creativa, consciente e inconsciente; de estar juntos, diferenciados,individualizados y separados; en un Eros y un Tánatos psicopedagógicos, psicoterapéuticos, humanos, libres yéticos. Seguín no solo sabía sino que sabía enseñar, en la integridad, la plenitud y la belleza. El amor para él es unelemento fundamental en su ser y hacer (Eros psicoterapéutico). Hay que ser capaces de dar y de darse, de amar y de pensar despertando vida interior. Comprender es mantener el espíritu abierto sin prejuicios ni rigideces, con ansia de descubrir, con una pasión lúcida, con el intento de encontrar respuesta a interrogantes, enigmas y misterios para aliviar el sufrimiento del hombre, integrando pasado y presente, favoreciendo el futuro. Para Carlos Alberto Seguínes tremendamente importante la relación con el paciente. Su orientación es de una medicina humanista, de ver alpaciente como una totalidad de alma y cuerpo frente a problemas de vida y muerte, reales y simbólicos, restaurandoel equilibrio perdido. Puente que une la terapia con la cultura. Concluye este trabajo el poema “El crimen y el castigo del crimen” del libro El Profeta de Khalil Gibran, que el doctor Seguín tradujo con sensibilidad y empatía. (RevNeuropsiquiatr 2010;73:164-169).
Síndrome de Townes-Brocks, depresión y adicción a inhalantes: a propósito de un caso.
Authors: Lozano-Vargas Antonio , Luna-León Fernando 
Published: March 2016

El síndrome de Townes-Brocks (TBS) es un trastorno autosómico dominante con múltiples malformaciones y deexpresión variable. Presentamos el caso de una mujer de 22 años de edad con malformaciones en el oído externo,ano imperforado, riñones en herradura, desviación cubital del pulgar, sindactilia en los dedos de ambos pies,estrabismo, útero bicorne y retardo mental; quien desarrolló distimia, depresión mayor y adicción a inhalantes. (RevNeuropsiquiatr 2010;73:170-175).
Epilepsia post ictus.
Authors: Toro-Pérez Juan E. , Villafuerte Mirla V. , Burneo Jorge G. 
Published: March 2016

En las personas mayores de 60 años, el ictus es la causa más frecuente de epilepsia. La tasa global de epilepsia postictus varía del 2% al 4%. La localización cortical y el tamaño de la isquemia son los factores que están asociadoscon crisis epilépticas post ictus. Las crisis epilépticas de inicio tardío se asocian frecuentemente con el desarrollode epilepsia post ictus, siendo su principal manifestación clínica las convulsiones parciales con generalizaciónsecundaria. Además de los estudios de imagen, el electroencefalograma es de considerable ayuda durante el inicio de crisis, así como durante el seguimiento, donde el patrón más frecuente son ondas lentas focales. El tratamiento con drogas antiepilépticas debe iniciarse con bajas dosis, y lentamente incrementarlas hasta alcanzar la dosis efectiva.Los nuevos fármacos son los preferidos, debido a su baja tasa de efectos colaterales.
Effect of inbreeding on pollen tube growth in diploid and tetraploid Chamerion angustifolium: Do polyploids mask mutational load in pollen?
Authors: Husband Brian C. 
Published: March 2016

PREMISE OF THE STUDY:Deleterious recessive mutations are an important determinant of fitness (mutational load) in the sporophytic phase of plants and a major cause of inbreeding depression; however, their role in gametophyte function is less well documented but may account for variation in pollen tube growth and siring ability, especially between diploid and polyploid plants, which can mask the load.METHODS:We investigated the role of mutational load in pollen performance using the perennial polyploid Chamerion angustifolium by comparing tube growth of pollen, in styles and in growth medium, from inbred (selfed) and outbred diploids to that of inbred and outbred tetraploids. Pollen from tetraploids is expected to mask deleterious mutations more effectively in the outbred condition but reveal them after inbreeding. In contrast, gametophytes from diploids should express the same genetic load in inbred or outbred plants.KEY RESULTS:Pollen tube growth measured in growth medium was highest in outbred tetraploids and generally lower in inbred than outbred plants. The effect of selfing was significant in pollen from tetraploids but not diploids. The differential effect of selfing was also evident in the proportion of pollen reaching the base of styles, but the ploidy × pollination interaction was not significant. Selfing also had a negative effect on sporophyte fitness but was greater in diploids than tetraploids.CONCLUSIONS:Pollen performance is influenced by the expression of mutational load, which is masked in polyploids. This effect may partly explain strong siring success of tetraploids in this species.
Pollen structure and function in caesalpinioid legumes
Authors: Banks Hannah , Rudall Paula J. 
Published: March 2016

PREMISE OF THE STUDY:A diverse range of pollen morphologies occurs within the large, paraphyletic legume subfamily Caesalpinioideae, especially among early‐branching lineages. Previous studies have hypothesized an association between surface ornamentation and pollination syndrome or other aspects of pollen function such as desiccation tolerance and adaptations to accommodate volume changes.METHODS:We reviewed caesalpinioid pollen morphology using light microscopy, scanning and transmission electron microscopy, in combination with a literature survey of pollination vectors.KEY RESULTS:Pollen structural diversity is greatest in the early‐branching tribes Cercideae and Detarieae, whereas Cassieae and Caesalpinieae are relatively low in pollen diversity. Functional structures to counter desiccation include opercula (lids) covering apertures and reduced aperture size. Structures preventing wall rupture during dehydration and rehydration include different forms of colpi (syncolpi, parasyncolpi, pseudocolpi), striate supratectal ornamentation, and columellate or granular wall structures that resist tensile or compressive forces respectively. Specialized aperture structures (Zwischenkörper) may be advantageous for efficient germination of the pollen tube.CONCLUSIONS:In Detarieae and Cercideae in particular, there is potential to utilize pollen characters to estimate pollination systems where these are unknown. Supratectal verrucae and gemmae have apparently evolved iteratively in Cercideae and Detarieae. At the species level, there is a potential correlation between striate/verrucate patterns and vertebrate pollination.
Studying activity in manual work: a framework for analysis and training
Authors: Le Bellu Sophie , Lahlou Saadi , Nosulenko Valery , Samoylenko Elena 
Published: March 2016

Cet article présente une méthode d’étude des savoir-faire incorporés dans les activités de travail manuel, à des fins de transmission pédagogique en situation de formation professionnelle. Cette méthode s’adresse donc à une catégorie spécifique d’activité humaine, celle des gestes professionnels. Son développement est le fruit d’une recherche empirique menée au sein d’un grand groupe énergétique français, pour sa branche formation, afin de répondre au double besoin généré par le contexte organisationnel de renouvellement intergénérationnel ; à savoir, capitaliser les connaissances rares et critiques des experts, et être en capacité de les transmettre aux nouveaux arrivants par le biais de solutions de formation numériques innovantes. Le cadre d’analyse se veut avant tout centré sur la perspective de l’expert. Il propose une chaîne opérationnelle assurant le recueil, l’analyse, la formalisation et la transmission effective du savoir-faire tacite et/ou explicite sous-jacent à la réalisation d’un geste professionnel. S’appuyant sur les récents développements de l’ethnographie numérique subjective, le protocole de recueil imbrique des observations vidéo situées de l’activité, en première et troisième personne, avec plusieurs protocoles de verbalisations, dont un simultané à la réalisation du geste et orienté-but. La mise en lien des données « objectives » (observations vidéo) et « subjectives » (ce que l’expert pense et dit) est assurée sur la base : des cadres d’analyse proposés par les théories de l’activité, afin de structurer l’activité ; et d’une approche dite de « qualité perçue ». Cette dernière vise à dégager les dimensions naturelles issues de la perception des affordances du contexte par les participants dont on étudie l’activité. L’application de cette méthode globale permet à l’analyste de construire un modèle cognitif du geste à transmettre. Ce dernier, assorti du matériel vidéo et verbal recueilli, sert de structure de base à la réalisation d’un support didactique véhiculant l’expérience réelle de l’expert.
Achieving Potent Autologous Neutralizing Antibody Responses against Tier 2 HIV-1 Viruses by Strategic Selection of Envelope Immunogens
Authors: Hessell Ann J. , Malherbe Delphine C. , Pissani Franco , McBurney Sean , Krebs Shelly J. , Gomes Michelle , Pandey Shilpi , Sutton William F. , Burwitz Benjamin J. , Gray Matthew , Robins Harlan , Park Byung S. , Sacha Jonah B. , LaBranche Celia C. , Fuller Deborah H. , Montefiori David C. , Stamatatos Leonidas , Sather D. Noah , Haigwood Nancy L. 
Published: March 2016

Abstract
Advancement in immunogen selection and vaccine design that will rapidly elicit a protective Ab response is considered critical for HIV vaccine protective efficacy. Vaccine-elicited Ab responses must therefore have the capacity to prevent infection by neutralization-resistant phenotypes of transmitted/founder (T/F) viruses that establish infection in humans. Most vaccine candidates to date have been ineffective at generating Abs that neutralize T/F or early variants. In this study, we report that coimmunizing rhesus macaques with HIV-1 gp160 DNA and gp140 trimeric protein selected from native envelope gene sequences (envs) induced neutralizing Abs against Tier 2 autologous viruses expressing cognate envelope (Env). The Env immunogens were selected from envs emerging during the earliest stages of neutralization breadth developing within the first 2 years of infection in two clade B–infected human subjects. Moreover, the IgG responses in macaques emulated the targeting to specific regions of Env known to be associated with autologous and heterologous neutralizing Abs developed within the human subjects. Furthermore, we measured increasing affinity of macaque polyclonal IgG responses over the course of the immunization regimen that correlated with Tier 1 neutralization. In addition, we report firm correlations between Tier 2 autologous neutralization and Tier 1 heterologous neutralization, as well as overall TZM-bl breadth scores. Additionally, the activation of Env-specific follicular helper CD4 T cells in lymphocytes isolated from inguinal lymph nodes of vaccinated macaques correlated with Tier 2 autologous neutralization. These results demonstrate the potential for native Env derived from subjects at the time of neutralization broadening as effective HIV vaccine elements.