bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electro‐chromatography with a methacrylate ester‐based monolithic column
Authors: Chen Zhao , Xu Aili , Bi Xiaoli , Luo Wenhui , Li Ji , Fan Guorong , Sun Dongmei 
Published: July 2017

AbstractIn the present study, a monolithic capillary column with higher permeability was developed for the in vivo discrimination of four coumarin analogs (bergapten, 2′‐acetylangelicin, imperatorin, and osthole) that typically require long separation times in HPLC. Instead of conventional methacrylate ester monolith (containing 19.5% porogen) with insufficient permeability (K = 1.52 – 1.66 × 10−14 M2) for plasma sample, the proposed column (20.5% porogen) had better permeability (around 3.80 × 10−14 M2) while properties such as pore distribution, stability, and resolution changed slightly. As a result, due to the negatively charged electro‐dynamic flow of the methacrylate ester groups in the monolith, the migration of targeted analytes was achieved within 6 min (compared with 30 min in HPLC) with acceptable resolution and improved sensitivity (0.005–0.02 μg/mL vs. 0.04 μg/mL). The proposed method was also applied to pharmacokinetic research: accelerated solvent extraction (ASE) was used to improve the extraction efficiency, which prepared extract much faster and more pure than conventional methods. As the pharmacokinetic parameters indicated, the monolithic capillary electro‐chromatography method was efficient, sensitive, specific, and durable, guaranteeing its utility for the determination of multiple structure‐related compounds in rat plasma.
Comparison of cell‐based versus cell‐free mammalian systems for the production of a recombinant human bone morphogenic growth factor
Authors: Jérôme Valérie , Thoring Lena , Salzig Denise , Kubick Stefan , Freitag Ruth 
Published: July 2017

AbstractThe human bone morphogenetic protein‐2 (hBMP2) is a glycoprotein, which induces de novo bone formation. Here, recombinant production in stably transfected Chinese Hamster Ovary (CHO) cells is compared to transient expression in Human Embryo Kidney (HEK) cells and cell‐free synthesis in CHO cell lysates containing microsomal structures as sites of post‐translational processing. In case of the stably transfected cells, growth rates and viabilities were similar to those of the parent cells, while entry into the death phase of the culture was delayed. The maximum achievable rhBMP2 concentration in these cultures was 153 pg/mL. Up to 280 ng/mL could be produced in the transient expression system. In both cases the rhBMP‐2 was found to interact with the producer cells, which presumably contributed to the low yields. In the cell‐free system, hBMP2 yields could be increased to almost 40 μg/mL, reached within three hours. The cell‐free system thus approached productivities for the active (renatured) protein previously only recorded for bacterial hosts, while assuring comprehensive post‐translational processing.
Ultrasound‐assisted swelling of bacterial cellulose
Authors: Song Ji Eun , Su Jing , Loureiro Ana , Martins Madalena , Cavaco‐Paulo Artur , Kim Hye Rim , Silva Carla 
Published: July 2017

AbstractBacterial cellulose (BC) was obtained by static cultivation using commercial BC gel from scoby. BC membranes (oven dried and freeze‐dried) were swelled with 8% NaOH, in the absence and in the presence of ultrasound (US), for 30, 60, and 90 min. The influence of swelling conditions on both physico‐chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30 min at room temperature in the presence of US. Native and PEGylated laccase from Myceliophthora thermophila was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5–7 U/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1–2 U/gBC). BC swelled membranes are presented herein as a potential support for the preparation of immobilized enzymes for industrial applications, like phenolics polymerization.
Psychometric Evaluation and Norms for the Multidimensional Assessment of Interoceptive Awareness (MAIA) in a Clinical Eating Disorders Sample
Authors: Brown Tiffany A. , Berner Laura A. , Jones Michelle D. , Reilly Erin E. , Cusack Anne , Anderson Leslie K. , Kaye Walter H. , Wierenga Christina E. 
Published: July 2017

AbstractAltered interoceptive awareness (IA) has been implicated in the pathophysiology of eating disorders; however, few comprehensive self‐report measures of IA exist in eating disorders. The present study sought to validate the Multidimensional Assessment of Interoceptive Awareness (MAIA), originally developed to assess IA in individuals practicing mind–body therapies, in an eating disorder sample. Adult and adolescent patients (n = 376) completed assessments upon admission to a partial hospital programme. Analyses examined the factor structure of the MAIA, scale means, scale–scale correlations, internal consistency and construct validity. Analyses also examined associations between MAIA subscales and eating disorder symptoms. Results supported the original eight‐factor structure of the MAIA. Internal consistency was acceptable, and the scales converged with associated measures. Importantly, Not Distracting, Self‐regulation, Body Listening and Trusting were most strongly associated with eating disorder symptoms. Results support use of the MAIA among eating disorders and provide further support for the relevance of IA in eating disorders. Copyright © 2017 John Wiley & Sons, Ltd and Eating Disorders Association.
Sustained lean transformation of working conditions: A Swedish longitudinal case study
Authors: Håkansson Malin , Dellve Lotta , Waldenström Måns , Holden Richard J. 
Published: July 2017

AbstractStudies of lean production's effect on working conditions are mixed but point toward worsened conditions. The aim of this longitudinal study was to assess how lean contributes to transforming work characteristics in a medium‐sized specialized industrial family business. A mixed methods approach was used to combine an external assessment of work characteristics with self‐reported employee questionnaires. Favorable psychosocial working conditions were indicated, with role conflicts decreasing over time. The assessment of work provided descriptions of how lean practices contributed to decreased task control due to standardized work and simplified work processes. At the same time, employees still had opportunities to influence their long‐term work assignment. There was also a trend toward job enlargement through new, higher‐skilled tasks, multiskilling, and extended opportunities to influence work through different continuous improvement activities. The participative lean approach and type of skilled jobs may have contributed to this transformation.
Efficacy of ultrasound and nerve stimulation guidance in peripheral nerve block: A systematic review and meta‐analysis
Authors: Wang Zhi‐Xue , Zhang De‐Li , Liu Xin‐Wei , Li Yan , Zhang Xiao‐Xia , Li Ru‐Hong 
Published: July 2017

AbstractEvidence was controversial about whether nerve stimulation (NS) can optimize ultrasound guidance (US)‐guided nerve blockade for peripheral nerve block. This review aims to explore the effects of the two combined techniques. We searched EMBASE (from 1974 to March 2015), PubMed (from 1966 to Mar 2015), Medline (from 1966 to Mar 2015), the Cochrane Central Register of Controlled Trials and clinicaltrials.gov. Finally, 15 randomized trials were included into analysis involving 1,019 lower limb and 696 upper limb surgery cases. Meta‐analysis indicated that, compared with US alone, USNS combination had favorable effects on overall block success rate (risk ratio [RR] 1.17; confidence interval [CI] 1.05 to 1.30, P = 0.004), sensory block success rate (RR 1.56; CI 1.29 to 1.89, P < 0.00001), and block onset time (mean difference [MD] –3.84; CI –5.59 to –2.08, P < 0.0001). USNS guidance had a longer procedure time in both upper and lower limb nerve block (MD 1.67; CI 1.32 to 2.02, P < 0.00001; MD 1.17; CI 0.95 to 1.39, P < 0.00001) and more patients with anesthesia supplementation (RR 2.5; CI 1.02 to 6.13, P = 0.05). USNS guidance trends to result in a shorter block onset time than US alone as well as higher block success rate, but no statistical difference was demonstrated, as more data are required. © 2017 IUBMB Life, 69(9):720–734, 2017
An improved correlation to predict molecular weight between crosslinks based on equilibrium degree of swelling of hydrogel networks
Authors: Jimenez‐Vergara Andrea C. , Lewis John , Hahn Mariah S. , Munoz‐Pinto Dany J. 
Published: July 2017

AbstractAccurate characterization of hydrogel diffusional properties is of substantial importance for a range of biotechnological applications. The diffusional capacity of hydrogels has commonly been estimated using the average molecular weight between crosslinks (Mc), which is calculated based on the equilibrium degree of swelling. However, the existing correlation linking Mc and equilibrium swelling fails to accurately reflect the diffusional properties of highly crosslinked hydrogel networks. Also, as demonstrated herein, the current model fails to accurately predict the diffusional properties of hydrogels when polymer concentration and molecular weight are varied simultaneously. To address these limitations, we evaluated the diffusional properties of 48 distinct hydrogel formulations using two different photoinitiator systems, employing molecular size exclusion as an alternative methodology to calculate average hydrogel mesh size. The resulting data were then utilized to develop a revised correlation between Mc and hydrogel equilibrium swelling that substantially reduces the limitations associated with the current correlation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1339–1348, 2018.
The evaluation of acute toxicity, antimicrobial activity of 1‐phenyl‐5‐p‐tolyl‐1H‐1, 2, 3‐triazole, and binding to human serum albumin
Authors: Duan Hong‐Ye , Li Jian‐Ling , Wu Lu‐Yong , Shu Huo‐Ming , Chen Yu‐Xue , Ding Guo‐Hua , Dong Run‐Cong , Si Hong‐Zong , Zhong Xia , He Wen‐Ying 
Published: July 2017

Abstract1‐Phenyl‐5‐p‐tolyl‐1H‐1, 2, 3‐triazole (PPTA) was a synthesized compound. The result of acute toxicities to mice of PPTA by intragastric administration indicated that PPTA did not produce any significant acute toxic effect on Kunming strain mice. It exhibited the various potent inhibitory activities against two kinds of bananas pathogenic bacteria, black sigatoka and freckle, when compared with that of control drugs and the inhibitory rates were up to 64.14% and 43.46%, respectively, with the same concentration of 7.06 mM. The interaction of PPTA with human serum albumin (HSA) was studied using fluorescence polarization, absorption spectra, 3D fluorescence, and synchronous spectra in combination with quantum chemistry and molecular modeling. Multiple modes of interaction between PPTA and HSA were suggested to stabilize the PPTA–HSA complex, based on thermodynamic data and molecular modeling. Binding of PPTA to HSA induced perturbation in the microenvironment around HSA as well as secondary structural changes in the protein.
Transversals in Latin Arrays with Many Distinct Symbols
Authors: Best Darcy , Hendrey Kevin , Wanless Ian M. , Wilson Tim E. , Wood David R. 
Published: July 2017

AbstractAn array is row‐Latin if no symbol is repeated within any row. An array is Latin if it and its transpose are both row‐Latin. A transversal in an array is a selection of n different symbols from different rows and different columns. We prove that every Latin array containing at least distinct symbols has a transversal. Also, every row‐Latin array containing at least distinct symbols has a transversal. Finally, we show by computation that every Latin array of order 7 has a transversal, and we describe all smaller Latin arrays that have no transversal.
The natural history of thin melanoma and the utility of sentinel lymph node biopsy
Authors: Durham Alison B. , Schwartz Jennifer L. , Lowe Lori , Zhao Lili , Johnson Andrew G. , Harms Kelly L. , Bichakjian Christopher K. , Orsini Amy P. , McLean Scott A. , Bradford Carol R. , Cohen Mark S. , Johnson Timothy M. , Sabel Michael S. , Wong Sandra L. 
Published: July 2017

Background and ObjectivesCurrent literature may overestimate the risk of nodal metastasis from thin melanoma due to reporting of data only from lesions treated with SLNB. Our objective was to define the natural history of thin melanoma, assessing the likelihood of nodal disease, in order to guide selection for SLNB.MethodsRetrospective review. The primary outcome was the rate of nodal disease. Clinicopathologic factors were evaluated to find associations with nodal disease.ResultsFive hundred and twelve lesions, follow up available for 488 (median: 48 months). Lesions treated with WLE/SLNB compared to WLE alone were more likely to have high‐risk features. The rate of nodal disease was higher in the WLE/SLNB group (24 positive SLNB, five false‐negative SLNB with nodal recurrence: 10.2%) compared to WLE alone (four nodal recurrences: 2.0%). Univariate analysis showed age ≤45, Breslow depth ≥0.85 mm, mitotic rate >1 mm2, and ulceration were associated with nodal disease. Multivariate analysis confirmed the association of age ≤45 and ulceration.ConclusionsSLNB for melanoma 0.75‐0.99 mm should be considered in patients age ≤45, Breslow depth ≥0.85 mm, mitotic rate >1 mm2, and/or with ulceration. Thin melanoma <0.85 mm without high‐risk features may be treated with WLE alone.
Modeling of bispecific antibody elution in mixed‐mode cation‐exchange chromatography
Authors: Lee Yi Feng , Kluters Simon , Hillmann Mirjam , von Hirschheydt Thomas , Frech Christian 
Published: July 2017

AbstractThe increasing demand for cost‐efficient manufacturing of biopharmaceuticals has been the main driving force for the development of novel chromatography resins, which resulted in the development of multimodal or mixed‐mode chromatographic resins. Most of them combine electrostatic and hydrophobic functionalities and are designed to deliver unique selectivity and increased binding capacities also at increased ionic strength. However, the mechanism of the protein–resin interaction in mixed‐mode chromatography is still not fully understood. The performance of protein separations in mixed‐mode chromatography is consequently difficult to predict. In this work, we present a model combining both salt and pH dependence to characterize and to predict protein retention in mixed‐mode chromatography. The model parameters are determined based on simple linear pH gradient elution experiments at different ionic strengths and they are directly transferable for the prediction of salt‐induced elution at fixed pH. Validity of the model is demonstrated for a bispecific antibody and its product‐related impurities.
Phytic acid induced three‐dimensional graphene for the enrichment of phthalate esters from bottled water and sports beverage samples
Authors: Liang Xinyu , Wang Juntao , Wu Juanjuan , Wu Qiuhua , Wang Chun , Wang Zhi 
Published: July 2017

AbstractA three‐dimensional graphene was synthesized through a hydrothermal reaction of graphene oxide with phytic acid. The microstructure and morphology of the phytic acid induced three‐dimensional graphene were investigated by nitrogen adsorption–desorption isotherms, scanning electron microscopy, and transmission electron microscopy. With a large surface area and three‐dimensional structure, the graphene was used as the solid‐phase extraction adsorbent for the extraction of phthalate esters from bottled water and sports beverage samples before high‐performance liquid chromatographic analysis. The results indicated that the graphene was efficient for the solid‐phase extraction of phthalate esters. The limits of detection (S/N = 3) of the method for the analytes were 0.02–0.03 ng/mL for the water samples and 0.03–0.15 ng/mL for the sports beverage sample. The limits of quantitation (S/N = 9) for the analytes were 0.06–0.09 ng/mL for water samples and 0.09–0.45 ng/mL for sports beverage sample. The calibration curves for the phthalate esters by the method had a good linearity from 0.1 to 80.0 ng/mL with correlation coefficients larger than 0.9997. The recoveries of the analytes for the method fell in the range of 86.7–116.2% with the relative standard deviations between 1.5 and 6.8%.
Salivary duct stenosis: Short‐term symptom outcomes after sialendoscopy‐assisted salivary duct surgery
Authors: Delagnes Elise A. , Zheng Melissa , Aubin‐Pouliot Annick , Chang Jolie L. , Ryan William R. 
Published: July 2017

ObjectivesTo prospectively assess short‐term symptom change after sialendoscopy‐assisted salivary duct surgery (SASDS) for salivary duct stenosis.Study DesignProspective cohort study.MethodsPatients with obstructive sialadenitis from duct stenosis completed the 20‐item Chronic Obstructive Sialadenitis Symptoms (COSS) Questionnaire (scored 0–100) prior to SASDS and 3 months postoperatively.ResultsThirty glands in 19 patients with endoscopically confirmed salivary duct stenosis showed overall symptom improvement, with a mean COSS score reduction of 12.9 points (standard deviation [SD] 13.1) to a mean postoperative score of 25.1 (range 0–75.5) (P < 0.001) with six (20%) glands (5 patients) achieving complete symptom resolution. Symptoms improved significantly for parotid glands (n = 20) by 16.6 points (SD 15.9) (P < 0.0001). For distal duct stenoses (n = 25), significant symptom improvement was seen in cases treated with dilation only (n = 17; partial stenoses) with a mean 20.6 point COSS reduction (SD 19.0) (P < 0.0005) and in cases treated with sialodochoplasty (n = 5; 4 complete, 1 partial stenosis) with a mean 13.8 point reduction (SD 4.7) (P < 0.005). Symptom scores did not improve after SASDS in proximal stenoses (n = 3) and distal stenoses cases not amenable to treatment (n = 3).ConclusionSASDS for salivary duct stenosis often can improve obstructive salivary symptoms; however, many patients report persistent symptoms after surgery. Partial duct stenoses or distal duct stenoses are associated with the greatest improvements in COSS scores after SASDS.Level of Evidence4. Laryngoscope, 127:2770–2776, 2017
Effect of psychosocial distress on outcome for head and neck cancer patients undergoing radiation
Authors: Chen Allen M. , Hsu Sophia , Felix Care , Garst Jordan , Yoshizaki Taeko 
Published: July 2017

Objectives/HypothesisTo determine the impact of pretreatment psychosocial distress on compliance to radiation therapy (RT) and clinical outcomes for patients with head and neck cancerStudy DesignSelf‐reported responses to the mood and anxiety domains of the University of Washington Quality of Life instrument were reviewed among 133 patients with newly diagnosed head and neck cancer prior to initiating RT.MethodsVarying definitions were used (total number of unexpectedly missed RT days, >5 days continuous interruption of RT outside of weekends, >10 days continuous interruption of RT, and failure to complete prescribed course of RT) to analyze the effect of psychosocial disruption on compliance. Survival was determined using the Kaplan‐Meier method.ResultsThe prevalence of pretreatment depression and anxiety was 23% and 47%, respectively. Continuous RT breaks >5 days occurred in 46%, 33%, 10%, 9%, and 0% of patients whose mood was rated as “extremely depressed,” “somewhat depressed,” “neither in a good mood or depressed,” “generally good,” and “excellent,” respectively (P = .0016). The corresponding proportion of patients who did not complete their planned RT was 23%, 11%, 5%, and 3%, and 0%, respectively (P = .043). The 2‐year overall survival of patients who were “extremely depressed” or “somewhat depressed” at baseline was 71% versus 86% for all others (P = .026). Depression was independently associated with decreased overall survival on logistical regression analysis.ConclusionsPretreatment depression predicted for decreased RT compliance and inferior survival for head and neck cancer. Additional research to overcome potential barriers to treatment in this setting may be warranted.Level of Evidence4. Laryngoscope, 128:641–645, 2018
Adjustable thermoplastic oral appliance versus positive airway pressure for obstructive sleep apnea
Authors: Banhiran Wish , Assanasen Paraya , Nopmaneejumrudlers Cherdchai , Nujchanart Nongyoaw , Srechareon Wimontip , Chongkolwatana Cheerasook , Metheetrairut Choakchai 
Published: July 2017

Objectives/HypothesisTo compare outcomes of continuous positive airway pressure (CPAP) and the adjustable thermoplastic mandibular advancement splint (AT‐MAS) for obstructive sleep apnea treatment.Study DesignRandomized crossover, noninferiority, tertiary center setting.MethodsFifty patients with a mean age of 49.5 ± 10.6 years were enrolled. Inclusion criteria were age ≥18 years, apnea‐hypopnea index (AHI) ≥5 events/hour, and oxygen saturation ≥70%. Exclusion criteria were temporomandibular joint disorders, severe periodontitis, inadequate teeth, and unstable medical diseases. Treatment intolerance was considered a failure. Two‐week periods without treatment were followed by questionnaires and randomization into two groups: CPAP/AT‐MAS (25) and AT‐MAS/CPAP (25). After 6 weeks of intervention, questionnaires and home WatchPAT monitoring were performed. Following each 2‐week washout period, patients crossed over to the other treatment followed by similar procedures. Primary outcomes involved the scores from the Functional Outcomes of Sleep Questionnaire (FOSQ). Secondary outcomes were AHI, side effects, and treatment adherence.ResultsSeven patients withdrew from this study: five (AT‐MAS intolerance) and two (lost follow‐up). There was no significant difference among FOSQ scores, particularly on global scores, between both treatments (0.57, 95% confidential interval of difference: −0.15 to 1.29). Mean AHI decreased from pretreatment 39.2 ± 2.53 to 2.56 ± 0.49 and 12.92 ± 2.05 events/hour while using CPAP and the AT‐MAS, respectively (P < .05). Most common side effects of CPAP were dry throat and inconvenience to carry, whereas those of the AT‐MAS were jaw pain and excessive salivation.ConclusionsBoth devices improved short‐term quality of life similarly; however, the AT‐MAS was not as efficacious as CPAP on resolving sleep‐test parameters. The AT‐MAS might be considered only a temporary treatment alternative.Level of Evidence1b. Laryngoscope, 128:516–522, 2018