bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal DNA methylation in parent–infant pairs impacted by intergenerational social adversity: An RCT of the Michigan Model of Infant Mental Health Home Visiting
Authors: Petroff Rebekah L. , Jester Jennifer , Riggs Jessica , Alfafara Emily , Springer Katherine , Kerr Natalie , Issa Meriam , Hall Alanah , Rosenblum Katherine , Goodrich Jaclyn M. , Muzik Maria ,   the Michigan Collaborative for Infant Mental Health Research
Published: September 2024

AbstractIntroductionEarly childhood development is a strong predictor of long‐term health outcomes, potentially mediated via epigenetics (DNA methylation). The aim of the current study was to examine how childhood experiences, punitive parenting, and an intergenerational psychotherapeutic intervention may impact DNA methylation in young children and their mothers.MethodsMothers and their infants/toddlers between 0 and 24 months were recruited at baseline (n = 146, 73 pairs) to participate in a randomized control trial evaluating the effectiveness of The Michigan Model of Infant Mental Health Home Visiting (IMH‐HV) parent–infant psychotherapy compared to treatment as usual. Baseline and 12‐month post‐enrollment data were collected in the family's home and included self‐report questionnaires, biological saliva samples, home environment observation, video‐taped parent–child interaction, and audio‐recorded interviews. Saliva DNA methylation was measured at the genes, nuclear receptor subfamily 3 group C member 1 (NR3C1), solute carrier family 6 member 4 (SLC6A4), brain‐derived neurotrophic factor (BDNF), and the genetic element, long interspersed nuclear element‐1 (LINE1).ResultsFor mothers, baseline methylation of BDNF, SLC6A4, NR3C1, or LINE1 was largely not associated with baseline measures of their childhood adversity, adverse life experiences, demographic characteristics related to structurally driven inequities, or to IMH‐HV treatment effect. In infants, there were suggestions that methylation in SLC6A4 and LINE1 was associated with parenting attitudes. Infant BDNF methylation suggested an overall decrease in response to IMH‐HV psychotherapy over 12 months.ConclusionsOverall, our findings suggest that the epigenome in infants and young children may be sensitive to both early life experiences and parent–infant psychotherapy.
Patuletin Ameliorates Inflammation and Letrozole–Induced Polycystic Ovarian Syndrome in Rats
Authors: Shah Syeda Farah , Noorali Samina , Faizi Shaheen , Jabeen Almas 
Published: September 2024

ABSTRACTConcerns about inflammation‐related issues affecting female reproductive health are growing. Chronic low‐grade inflammation in women with polycystic ovarian syndrome (PCOS) affects follicular growth, ovulation, and androgen production. The present investigation aimed to elucidate the efficacy of flavonoid patuletin in ameliorating the letrozole–induced PCOS and associated inflammation in rats. Female Wistar rats (32 days old) were divided into five groups (n = 12): Group I, control; Group II, vehicle control; Group III, letrozole oral (1 mg/kg) for 28 days; Group IV and Group V treatment groups, patuletin i.p. (25 mg/kg) and clomiphene citrate + metformin i.p. (50 mg/kg + 300 mg/kg), respectively. Leterozole–induced PCOS and ovarian inflammation were ameliorated by patuletin, as reflected in the improved histopathology, prevention of cyst formation, significant upregulation of growth factors such as growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein‐15 (BMP‐15) expression, and a decrease in the pro‐inflammatory cytokines TNF‐α, IL‐6, and COX‐2. Additionally, the plasma levels of reproductive hormones were restored. Upregulation of FSH‐R, PR, and CYP19a1, along with downregulation of ERα, LHR, CYP17a1, CYP11a1 and HSDβ17a1, showed the regulation of gonadotropin receptors and steroid biosynthesis genes in ovarian tissues. Patuletin demonstrated a promising protective approach against the biological model of PCOS by increasing the inflammation in ovarian tissues with consequent regulation of growth factors, enzymes, and hormones, and might be used as adjuvant therapy in the treatment of problems related to female reproductive health.
Percutaneous closure of coronary fistulas in adults using microvascular plugs
Authors: Unzué Leire , Wasniewski Samantha , García Eulogio , Teijeiro‐Mestre Rodrigo 
Published: September 2024

AbstractPercutaneous closure of coronary fistulas can be a challenge, given the tortuosity and the small caliber of the anomalous vessel. The microvascular plugs (MVP) are polytetrafluoroethylene‐coated nitinol devices designed to perform embolization of small peripheral vessels. Its reduced profile allows the release of the device through microcatheters, facilitating the intervention. We present three cases of coronary fistulas in adults, percutaneously closed through radial access using these devices.
Localized retroperitoneal mass suspected malignancy: A rare case of unicentric Castleman's disease
Authors: Alhariry Engy S. , Hasan Abdulkarim , Abdelghany Ashraf , Nafie Khalid 
Published: September 2024

Key Clinical MessageCastleman's Disease should be considered in the differential diagnosis of retroperitoneal masses, especially in equivocal cases. Clinician should not presume all cases of retroperitoneal masses as a malignancy.AbstractCastleman's Disease is a heterogeneous group of lymphoproliferative disorders, that can develop in lymph nodes or in extranodal sites. It has three distinct histological subtypes; hyaline vascular, plasma cell or mixed. It can be unicentric or multicentric, and sometimes oligocentric or regional. In this article, we report a case of a 30‐year‐old male who presented with a palpable left lumbar mass, clinically suspected as sarcoma vs GIST, which was surgically excised and pathologically examined revealing a rare condition of intra‐abdominal unicentric Castleman's Disease with good prognosis. Castleman's Disease should be considered in the differential diagnosis of retroperitoneal masses, especially in equivocal cases.
Mononuclear and Multinuclear O^N^O‐donor Zn(II) Complexes as Robust Catalysts for the Production and Depolymerization of Poly(Lactide)
Authors: Zikode Mnqobi , Fuchs Martin , Langletz Tim , Burkart Lisa , Herres‐Pawlis Sonja , Ojwach Stephen O. 
Published: September 2024

AbstractMononuclear and multinuclear O^N^O‐donor Zn(II) complexes are herein reported as catalysts in the ring‐opening polymerization (ROP) of rac‐lactide (rac‐LA) and depolymerization of poly(lactide) (PLA). Reactions of imino‐phenol proligand 2[((2‐hydroxyethyl)imino)methyl]phenol (L1H2) with ZnCl2 and ZnEt2 afforded the dinuclear and tetranuclear complexes [Zn2(L1H)2Cl2] (Zn1) and [Zn4(L1)4] (Zn2), respectively. Separately, treatments of proligands 2‐[1‐((2‐hydroxyethyl)imino)ethyl]phenol (L2H2) and [((2‐methoxyethyl)imino)methyl]phenol (L3H) with ZnCl2 gave the respective mononuclear complexes [Zn(L2H2)2Cl2] (Zn3) and [Zn(L3H)2Cl2] (Zn4), while the reactions of L3H with ZnEt2 afforded the bis(chelated) mononuclear complex [Zn(L3)2] (Zn5). The complexes were characterized using NMR and IR spectroscopy, elemental analyses and single crystal X‐ray crystallography. All the Zn(II) complexes showed high catalytic activities in the ROP of rac‐LA (kapp of up to 4.18 × 10−4 s−1) to give moderate molecular weight PLA (39,960 g mol−1) with relatively narrow dispersities (Đ = 1.4–2.1). The complexes produced mainly atactic PLAs with Pr values of 0.51–0.58. More significantly, complexes Zn1, Zn2, and Zn5 were also active in the depolymerization of commercial PLA in methanol (kapp of up to 2.67 × 10−4 s−1) to produce alkyl lactates. The ROP and depolymerization behavior were largely controlled by the structure and nuclearity of the complexes.
Biosynthesis of Nonribosomal Peptides Chitinimides Reveal a Special Type of Thioesterase Domains
Authors: Niu Weijing , Liu Jiaqi , Duan Yuwei , Zhong Lin , Pang Linlin , Zhong Guannan , Zhang Youming , Bian Xiaoying 
Published: September 2024

AbstractNon‐ribosomal peptide synthetases (NRPSs) and their tailored enzymes have diverse biological functions. In this study, we investigated the biosynthesis and function of chitinimides, which belong to the non‐ribosomal peptide (NRP) subfamily featuring a pyrrolidine‐containing part (X part) connected to the polypeptide chain via an ester bond. A conserved gene cassette, chmHIJK, is responsible for oxyacylation of the pyrrolidine moiety in the X part. The thioesterase (TE) domain of ChmC (ChmC‐TE) catalyzes transesterification reactions with a free X part or methanol as a nucleophilic reagent to form different chitinimides. The crucial amino acid residues in the ChmC‐TE domains responsible for the specific recognition of the X part were identified, and they were conserved in all the biosynthetic pathways of this NRP subfamily to form a signature motif, YNHNR, suggesting a special type of TE domain in NRPSs. Chitinimides demonstrate the biological function of promoting the swarming ability of the native producer. This study provides deep insights into the biosynthesis of this special NRP subfamily, and shows that the special TE domain could be used to generate diverse NRPs by combinatorial biosynthesis.
Effectiveness of Imagery Rescripting for Trauma‐Affected Voice Hearers: An Open Trial
Authors: Strachan Laura , McEvoy Peter , Arntz Arnoud , Steel Craig , Paulik Georgie 
Published: September 2024

ABSTRACTObjectivePeople who hear voices (auditory verbal hallucinations) often have post‐traumatic stress disorder (PTSD) symptoms. Cognitive behavioural therapies (CBT) have yielded inconsistent findings when treating voices and PTSD symptoms in voice hearers. Preliminary evidence suggests imagery rescripting (ImRs) is associated with large reductions in voice hearing and PTSD symptoms. This study replicated past studies using a larger sample to examine the effectiveness of ImRs in reducing such symptoms.MethodParticipants (N = 49; 65.3% female; Mage = 35.86) were clients at an Australian transdiagnostic clinic for voice hearers. A one‐arm open trial design was used with three pre‐treatment baselines and a mid‐treatment, post‐treatment and 3‐month follow up assessments of PTSD symptoms (Posttraumatic Diagnostic Scale for DSM‐5), voices (Hamilton Program for Schizophrenia Voices Questionnaire) and emotional symptoms (Depression Anxiety and Stress Scales‐21). Five single‐item measures were administered weekly to explore the trajectories of change in trauma intrusions, voice‐related distress, voice frequency and positive and negative voice valance.ResultsImRs was associated with very large reductions in PTSD symptoms and voices (both emotional and physical characteristics of voices) and emotional symptoms at post‐treatment and follow‐up (η2p = 0.24–0.44). There were medium‐large to large reductions in weekly symptoms of intrusions, voice‐related distress, voice frequency and negative voices (η2p = 0.12–0.16) and a non‐significant increase in positive voices (η2p = 0.05).ConclusionsThis study provides further evidence that ImRs is an effective treatment for voices and PTSD symptoms in voice hearers with a range of diagnoses. Randomised controlled trials are needed to compare the efficacy of ImRs to CBT protocols.
Design and Synthesis of Aggregation‐Caused Quenching Hydroxy‐Phenanthroimidazole Derivatives for Probing of Fe3+ Ions and as Potential Blue Light Emitters
Authors: Odedara Nisha , Borane Niteen , Boddula Rajamouli 
Published: September 2024

AbstractFluorescence aggregated molecules tend to employ versatile opportunities in metal ion probe sensors and fluorescent lighting. To achieve this dual challenging task, currently synthesized three phenanthroimidazole‐naphthalene‐based compounds Pq‐tBu‐OH, Pq‐mF‐OH, and Pq‐pF‐OH are derived based on substitution at N1 position for better photophysical and electrochemical properties. Compared experimental and theoretical calculations define the highest bandgap to be 2.75 eV of Pq‐pF‐OH, and the same molecule expressed a higher (348 °C) thermal decomposition. The calculated singlet and triplet energies found in the range of 3.24–3.67 and 2.70–2.72 eV indicate well energy transfer from S1→S0 (quantum yield of 23.36 %, lifetime is 4.05 ns). Among the numerous morphologies, the solid form exhibited improved intensive deep blue emission (x=0.159, y=0.051), and its InGaN LED results demonstrated a strong deep blue emission at 418 nm. Moreover, the fluorophores were experimentally visualizing the aggregation‐caused quenching (ACQ) which enables the probing of Fe3+ ion. However, for the first time, the ACQ‐assisted concept is applied through synthesized molecules for Fe3+ ion probing via fluorescence spectra, Job's plot calculation, and 1H NMR results. In addition, the probe works excellently at a detection limit of 10 μM and it could also act as a potential competitor for lighting applications.
In Vitro Effect of Anodization of Titanium Abutments on Color Parameters and Color Difference of Lithium Disilicate All‐Ceramic Crowns
Authors: Khorshidi Sotude , Zarbakhsh Arash , Lawaf Shirin , Golalipour Shaghayegh , Sayyari Maryam , Nahavandi Alireza Mahmoudi 
Published: September 2024

ABSTRACTObjectiveThis study assessed the effect of the anodization of titanium abutments on the color parameters and color difference of lithium disilicate (LDS) all‐ceramic crowns.Materials and MethodsIn this study, 19 straight abutments were divided into two groups: anodized (n = 9) and non‐anodized control (n = 9), with one hybrid zirconia abutment as a reference. Anodization was achieved by applying 63 V energy using seven 9 V flat batteries in series, with an electrolyte solution comprising 1 g trisodium phosphate in 250 mL distilled water for 5 s, resulting in a gold‐yellow color. Abutments were then scanned, and full‐contour monolithic IPS e.max maxillary central incisor crowns were fabricated with 2 mm thickness and glazed. Reflectance was measured using a spectroradiometer, and color coordinates (L*, a*, b*, h*, and C*) were calculated using CS‐10W software. Color differences of the crowns in both groups were quantified using the CIEDE2000 (ΔE00) color difference formula and analyzed by t‐test (α = 0.05) compared to the standard sample.ResultsThe L*, a*, b*, and c* parameters in anodized abutments were significantly higher than those in non‐anodized abutments, while the h* parameter in anodized abutments was significantly lower than that in non‐anodized abutments (p < 0.001 for all). There was a significant difference in ΔE00 of the two groups (p = 0.043).ConclusionAnodization of titanium abutments improved the color parameters of LDS all‐ceramic crowns and significantly decreased their ΔE compared with non‐anodized abutments.
Effect of Needle Insertion Angle on Pain During Labial Infiltration Anesthesia of the Anterior Maxilla: A Randomized Clinical Trial
Authors: Moaddabi Amirhossein , Molania Tahereh , Arezoumandi Alireza , Ghaedsharaf Sahar , Cernera Mariangela , Nikbakht Roya , Soltani Parisa , Spagnuolo Gianrico , Shahnaseri Shirin 
Published: September 2024

ABSTRACTObjectivesThis study aimed to assess the effect of needle insertion angle on pain during labial infiltration anesthesia in the anterior maxillary region.Material and MethodsIn this parallel‐design randomized clinical trial, participants were randomly assigned to four groups for labial infiltration anesthesia of the anterior maxilla. Local anesthesia was performed with needle orientation parallel to the longitudinal axis of the tooth using a conventional syringe (Syringe‐0), needle at α angle with a conventional syringe (Syringe‐α), computer‐controlled local anesthetic delivery (CCLAD) device parallel to the longitudinal axis of the tooth (CCLAD‐0), and CCLAD at α angle (CCLAD‐α). The heart rate (HR), blood pressure (BP), and respiratory rate (RR) of participants were measured before needle insertion, immediately after needle insertion, and immediately after the injection by a vital signs monitor. The level of pain experienced by participants was quantified using a numerical rating scale (NRS). Data were analyzed by repeated‐measures ANOVA and regression models (α = 0.05).ResultsThirty‐six participants aged from 21 to 60 years, with a mean age of 35.36 years were recruited. The mean pain scores were 7.44, 4.67, 2.89, and 0.67 in groups Syringe‐0, Syringe‐α, CCLAD‐0, and CCLAD‐α, respectively (p < 0.001). Age and sex had no significant effect on pain scores (p = 0.914 and p = 0.702, respectively). The four groups had no significant difference in vital signs (p > 0.05).ConclusionsInjection at an α angle and the application of CCLAD can be used in clinical practice to decrease the pain experienced by participants during labial infiltration anesthesia of the anterior maxilla.Trial RegistrationIranian Registry of Clinical Trials: IRCT20230719058849N1.
Knowledge and Attitude of General Dentists, Senior Dental Students, and Orthodontic Residents Toward Obstructive Sleep Apnea
Authors: Shekarian Mina , Feizbakhsh Masood , Rafie Mehdi 
Published: September 2024

ABSTRACTObjectiveThe main objective of this study was to compare the knowledge and attitude of general dentists, senior dental students, and orthodontic residents toward obstructive sleep apnea (OSA).Materials and MethodsA questionnaire was designed and administered among 73 senior dental students, 84 general dentists, and 50 orthodontic residents. The questionnaire asked for demographic information of the participants and assessed their knowledge and attitude toward OSA. The validity and reliability of the questionnaire were confirmed by experts. Data were analyzed by the Kruskal−Wallis and Bonferroni tests using SPSS (α = 0.05).ResultsThe mean knowledge scores of general dentists and senior dental students were significantly lower than those of orthodontic residents (p < 0.001). However, there was no significant difference in the knowledge score of senior dental students and general dentists (p = 1). The mean knowledge score was significantly higher in dentists with 1–3 years of professional clinical experience (p = 0.02). The knowledge score was the highest in dentists working in private clinics followed by private offices and public clinics. The mean attitude score was the highest in orthodontic residents followed by general dentists and dental students. There was no significant difference in attitude based on the attended university, age, or time of graduation.ConclusionAccording to the results, general dentists had insufficient knowledge about OSA, which could result in under‐diagnosis or misdiagnosis of patients with OSA. This finding highlights the need for further education and training for dentists to properly identify and refer OSA patients to orthodontists or sleep specialists.
Paleopteran molecular clock: Time drift and recent acceleration
Authors: Osozawa Soichi , Nel André 
Published: September 2024

AbstractApplying BEAST v1.10.4, we constructed a Bayesian Inference tree comprising 322 taxa, primarily representing Paleoptera (Odonata and Ephemeroptera; Pterygota), Zygentoma and Archaeognatha (Apterygota; paraphyly), and Neoptera (Plecoptera; Pterygota), based on a 2685 bp sequence dataset. Our analyses revealed that robust dating required the incorporation of both Quaternary and pre‐Quaternary dates. To achieve this, our dating incorporated a 1.55 Ma (Quaternary) geological event (the formation of the Ryukyu Islands) and a set of chronologically well‐founded fossil dates, spanning from up to 400 Ma (Devonian) for the stem Archaeognatha, 320 Ma (Carboniferous) for the crown of Paleoptera, 300 Ma (Carboniferous) for the crown Ephemeroptera, and 280 Ma (Permian) for the crown Odonata, down to 1.76 Ma (Quaternary) for Calopteryx japonica, encompassing a total of 22 calibration points (events: 6, fossils: 16; Quaternary: 7, pre‐Quaternary: 15). The resulting dated tree aligns with previous research, albeit with some dates being overestimated. This overestimation was mainly due to the lack of Quaternary calibration and the exclusive dependence on pre‐Quaternary calibration, though the application of maximum age constraints also played a role. Our minimum age dating demonstrates that the molecular clock did not uniformly progress, rendering rate dating an inapplicable approach. We observed that the base substitution rate is time‐dependent, with an exponential increase evident from around 20 Ma (Miocene) to the present time, exceeding an order of magnitude. The extensive radiation and speciation of Insecta and Paleoptera, potentially resulting from the severe climatic changes associated with the Quaternary, including the commencement of glacial and interglacial cycles, may have significantly contributed to this increase in base substitution rates. Additionally, we identified a potential peak in base substitution rates during the Carboniferous period, around 320 million years ago, possibly corresponding to the Late Paleozoic Ice Age.
Key benthic species are affected by predicted warming in winter but show resistance to ocean acidification
Authors: Schertenleib Katrin S. H. , Davey Tallulah , Taylor David , O'Connor Nessa E. 
Published: September 2024

AbstractThe effects of climate change on coastal biodiversity are a major concern because altered community compositions may change associated rates of ecosystem functioning and services. Whilst responses of single species or taxa have been studied extensively, it remains challenging to estimate responses to climate change across different levels of biological organisation. Studies that consider the effects of moderate realistic near‐future levels of ocean warming and acidification are needed to identify and quantify the gradual responses of species to change. Also, studies including different levels of biological complexity may reveal opportunities for amelioration or facilitation under changing environmental conditions. To test experimentally for independent and combined effects of predicted near‐future warming and acidification on key benthic species, we manipulated three levels of temperature (winter ambient, +0.8 and +2°C) and two levels of pCO2 (ambient at 450 ppm and elevated at 645 ppm) and quantified their effects on mussels and algae growing separately and together (to also test for inter‐specific interactions). Warming increased mussel clearance and mortality rates simultaneously, which meant that total biomass peaked at +0.8°C. Surprisingly, however, no effects of elevated pCO2 were identified on mussels or algae. Moreover, when kept together, mussels and algae had mutually positive effects on each other's performance (i.e. mussel survival and condition index, mussel and algal biomass and proxies for algal productivity including relative maximum electron transport rate [rETRmax], saturating light intensity [Ik] and maximum quantum yield [Fv/Fm]), independent of warming and acidification. Our results show that even moderate warming affected the functioning of key benthic species, and we identified a level of resistance to predicted ocean acidification. Importantly, we show that the presence of a second functional group enhanced the functioning of both groups (mussels and algae), independent of changing environmental conditions, which highlights the ecological and potential economic benefits of conserving biodiversity in marine ecosystems.