bluuebunny/crossref_metadata_2025_split huggingface dataset:
CANOPEX: A Canadian hydrometeorological watershed database
Authors: Arsenault Richard , Bazile Rachel , Ouellet Dallaire Camille , Brissette François 
Published: April 2016

AbstractOver the past few years, many international initiatives and collaborations were launched to improve and share knowledge in hydrology research. Large databases allowed finding patterns and relationships across regions and scales. This paper introduces the Canadian model parameter experiment (CANOPEX) database, which is adapted from the US MOPEX project data and methods. The CANOPEX database includes meteorological and hydrometric data as well as watershed boundaries for 698 basins. Two sets of basin‐averaged meteorological data (Maximum and minimum temperature and precipitation) are provided. The first dataset is directly taken from Environment Canada's weather stations whereas the second is extracted from the Natural Resources Canada gridded climate data product. Data are provided in MOPEX and Matlab formats. CANOPEX watersheds are well distributed over Canada, which allows investigating a variety of physiological and climatological conditions. The CANOPEX database can be used in a variety of hydrologic research projects such as climate change impact studies, model comparisons, multi‐modelling, ensemble streamflow prediction and model parameter estimation. CANOPEX could be used to generalize findings to other cold climate catchments as well as assess the robustness of research methodologies and procedures. Copyright © 2016 John Wiley & Sons, Ltd.
Variant RHD Types in Brazilians With Discrepancies in RhD Typing
Authors: Campos Fernanda Carolina Alves , Mota Mariza Aparecida , Aravechia Maria Giselda , Torres Kelyan Bertani , Bub Carolina Bonet , Kutner José Mauro , Castilho Lilian 
Published: April 2016

BackgroundThe knowledge of D variants in patients and donors is important because anti‐D alloimmunization can occur in some but not all individuals who express a variant RHD allele. Serologic distinction of RhD discrepancies is not always straightforward, which makes molecular analysis highly desirable.MethodsA group of 223 subjects, 129 patients, and 94 blood donors was identified and analyzed on the basis of a D typing discrepancy. The D antigen expression was evaluated by tube and gel hemagglutination with four anti‐D reagents. PCR‐single specific primer (SSP), multiplex PCR, RHD BeadChip (Immucor), or sequencing were used for molecular analysis.ResultsIn total, 168/223 (75%) weak D and 55/223 (25%) partial D variants were identified. Hemagglutination results varied in methods and anti‐D reagents used in this process. There was no standard serologic reactivity identified, which could predict what type of D variant would be identified. Among weak D samples, types 1–3 were the most common, while DAR and DVI were most prevalent among partial D samples.ConclusionOur results show that discrepancies found in the serologic typing should be investigated by molecular methods in order to determine the D variant involved and also to distinguish between weak D and partial D. The knowledge of the distribution of weak D types and partial D among populations is important for D− patients and pregnant women management.
A New Immunodot Assay for Multiplex Detection of Autoantibodies in a Cohort of Italian Patients With Idiopathic Inflammatory Myopathies
Authors: Tampoia Marilina , Notarnicola Antonella , Abbracciavento Letizia , Fontana Antonietta , Giannini Margherita , Louis Humbel Renè , Iannone Florenzo 
Published: April 2016

BackgroundAutoantibody detection has been assessed as tool for the diagnosis and the definition of idiopathic inflammatory myopathies (IIM). The aim of the study was to characterize the autoantibody profiling of a cohort of Italian patients with IIM.MethodsSera of 53 adult patients with definite IIM, according to Bohan–Peter criteria, were tested for anti‐nuclear autoantibodies (ANA), using indirect immunofluorescence (IIF) method, and for myositis‐specific autoantibodies (MSAs) and myositis‐associated autoantibodies (MAAs), using two new commercial immunodot assays.ResultsMSAs and/or MAAs were detected in 29 of 53 (54.7%) patients with IIM. Twenty‐three patients (43.4%) were positive for at least one MSAs: 13 (24.5%) had anti‐histidyl‐tRNA synthetase autoantibodies (Jo1), 4 (7.5%) had other anti‐aminoacyl‐tRNA synthetases autoantibodies (anti‐ARS), 1 (1.8%) had anti‐transcription intermediary factor 1 gamma autoantibodies (anti‐TIF1γ), 2 (3.7%) had anti‐nuclear helicase protein Mi‐2 autoantibodies (anti‐Mi‐2), 4 (7.5%) had anti‐small ubiquitin like modifier activating enzyme heterodimer autoantibodies (anti‐SAE). Moreover, 17 patients (32%) were positive for at least one MAAs. Coexisting MSAs and MAAs were observed in 9 of 53 (16.9%) patients, anti‐Jo1/SS‐A autoantibodies in most cases. Overall sensitivity of immunodot assays was 54.7%, the specificity was almost absolute. At cut‐off value of 1:160, the sensitivity of ANA‐IIF was 52.8%, increasing to 66% if cytoplasmatic fluorescence reaction was reported. Notably, two (5.7%) ANA‐IIF negative patients had MSAs, detected only by immunodot assays.ConclusionIt was possible to identify MSAs otherwise undetectable because of the use of new assays. Immunodot can reveal MSAs even when IIF results are inconclusive or, in some cases, ANA negative.
Association of Serum Albumin Level and Mortality in Premature Infants
Authors: Torer Birgin , Hanta Deniz , Yapakci Ece , Gokmen Zeynel , Parlakgumus Ayse , Gulcan Hande , Tarcan Aylin 
Published: April 2016

BackgroundHypoalbuminemia has been proven to be a powerful predictor of mortality in adult patients. However, prognostic value of serum albumin in neonates is not clear.ObjectiveTo assess the relationship between serum albumin level within the first day of life and outcome in preterm infants born before 32 weeks of gestation.MethodsThe study was conducted prospectively in Baskent University Hospital between October 2008 and November 2009. Patients were divided by gestational age into two groups as below or of 28 weeks and above 28 weeks. Then serum albumin percentile groups were established within each gestational age group and were defined as <25, 25–75, and >75 percentile groups by combining percentile groups between the two gestational age groups. Three serum albumin percentile groups were compared regarding neonatal outcomes.ResultsA total of 199 infants with mean birth weight of 1,272 ± 390 g and mean gestational age of 29.2 ± 2.2 weeks were admitted to the study. The mean serum albumin level was 30.6 ± 4.7 g/l for all patients. The mean serum albumin levels were 25.5 ± 3.8, 30.1 ± 2.7, and 35.3 ± 3.7 g/l for <25, 25–75, and >75 percentile groups, respectively. Prevalence of infants with respiratory distress syndrome and prevalence of infants with sepsis and mortality were significantly higher in <25 percentile group. Logistic regression analysis showed that serum albumin <25 percentile and birth weight were independent predictive variables of mortality. Albumin concentrations lower than 27.2 g/l was associated with mortality, with a sensitivity of 71% and a specificity of 86%.ConclusionLow serum albumin level within the first day of life is an independent predictor of mortality in preterm infants.
Colorimetric Detection by Gold Nanoparticle DNA Probes for Miltenberger Series (GP.Mur, GP.Hop, and GP.Bun) Identification
Authors: Vongsakulyanon Apirom , Pipatpanukul Chinnawut , Kitpoka Pimpun , Kunakorn Mongkol , Srikhirin Toemsak 
Published: April 2016

BackgroundMiltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi series blood typing, colorimetric gold nanoparticle (AuNP) DNA probes were therefore explored for Mi series identification.MethodsAuNPs were synthesized and conjugated to an RvB (test) probe and an RvA2 (control) probe. Each of the AuNP DNA probes was tested against the amplified products of Mi(+) (GP.Mur/Hop/Bun), Mi(–), and the blank (no amplified product). The change in color was observed by visual inspection and UV‐Vis spectroscopy.ResultsThe amplified product of the Mi(+) sample retained the color on both probes (test+/control+). The amplified product of the Mi(–) sample retained the color only on the control probe (test–/control+) and the amplified product of the blank turned clear on both probes (test–/control–). The results by optical density absorbance measurement were concordant with the results by visual inspection. Both probes were validated with the amplified products of the ten Mi(+) and ten Mi(–) samples. All of the samples were correctly identified.ConclusionAuNP DNA probes (RvB and RvA2) could be applied to distinguish the amplified products of Mi(+), Mi(–), and the blank by visual inspection and/or OD absorbance measurement.
Comparison of Two Methods for Determination of NGAL Levels in Urine: ELISA and CMIA
Authors: Krzeminska E. , Wyczalkowska‐Tomasik A. , Korytowska N. , Paczek L. 
Published: April 2016

BackgroundNeutrophil gelatinase‐associated lipocalin (NGAL) is a new useful biomarker for the early diagnosis of acute kidney injury. The aim of the study was to compare two analytical methods for measurement of urinary NGAL: enzyme‐linked immunosorbent assay (ELISA) and chemiluminescent microparticle immunoassay (CMIA).MethodsTwo assays were used to measure urinary NGAL: ELISA kit (R&D Systems) and ARCHITECT Urine NGAL (Abbott Laboratories). The study material was the urine obtained from 30 healthy subjects (mean age 56.4 ± 15.2).ResultsThe median value and interquantile range of urinary NGAL in the studied group measured by ELISA (R&D Systems) were 3.5 ng/ml (1.2; 6.6) and by CMIA (ARCHITECT Urine NGAL assay, Abbott Diagnostics) were 4.4 ng/ml (1.9; 9.4). Levels of urinary NGAL obtained by CMIA were significantly higher than by ELISA. There was a significant positive correlation between the concentration of urinary NGAL determined by both methods (r = 0.8625, P < 0.01).ConclusionThe comparison of individual data obtained by ELISA and CMIA should be taken with care. From laboratory's point of view, ELISA is less expensive than CMIA method for the determination of NGAL in urine. However, CMIA method allows rapid determination of urinary NGAL concentration through automated assay.
Survival following synchronous colon cancer resection
Authors: Thiels Cornelius A. , Naik Nimesh D. , Bergquist John R. , Spindler Blake A. , Habermann Elizabeth B. , Kelley Scott R. , Wolff Bruce G. , Mathis Kellie L. 
Published: April 2016

BackgroundSynchronous colon cancers, defined as two or more primary colon cancer detected simultaneously at the time of initial diagnosis, account for up to 5% of all colon cancer diagnoses. Management principles and outcomes remain largely undefined.MethodsA retrospective institutional review of patients undergoing curative intent resection for colon adenocarcinoma (Stages I–III) from 1995 to 2007 was performed. Hereditary causes or inflammatory bowel disease were excluded. Matching was performed and Kaplan–Meier analysis was used to compare overall survival.ResultsOf 2,387 patients, 100 (4.2%) had synchronous cancers. Patients with synchronous lesions tended to be older (median 77 vs. 72 years, P < 0.001) with more advanced tumors (41.0% vs. 31.4% Stage III, P = 0.04). After matching, there were no differences in demographics or tumor factors (all P > 0.05). Compared to solitary, synchronous cancers demonstrated an inferior 10‐year overall survival (53.9% vs. 36.5%, P = 0.009). Subset analysis of patients with synchronous cancers showed no difference in overall survival between those with extended versus segmental resections at 120‐months (P = 0.07).ConclusionSynchronous colon cancer is associated with decreased overall survival compared to patients with solitary tumors. Extended resection does not confer a survival benefit in these patients. Further research is needed to determine how to mitigate the poor outcomes. J. Surg. Oncol. 2016;114:80–85. © 2016 Wiley Periodicals, Inc.
Improving the adoption of thyroid cancer clinical practice guidelines
Authors: Likhterov Ilya , Tuttle R. Michael , Haser Grace C. , Su Henry K. , Bergman Donald , Alon Eran E. , Bernet Victor , Brett Elise , Cobin Rhoda , Dewey Eliza H. , Doherty Gerard , Dos Reis Laura L. , Klopper Joshua , Lee Stephanie L. , Lupo Mark A. , Machac Josef , Mechanick Jeffrey I. , Milas Mira , Orloff Lisa , Randolph Gregory , Ross Douglas S. , Rowe Meghan E. , Smallridge Robert , Terris David , Tufano Ralph P. , Urken Mark L. 
Published: April 2016

Objectives/HypothesisTo present an overview of the barriers to the implementation of clinical practice guidelines (CPGs) in thyroid cancer management and to introduce a computer‐based clinical support system.Data SourcesPubMed.Review Methods: A review of studies on adherence to CPGs was conducted.ResultsAwareness and adoption of CPGs is low in thyroid cancer management. Barriers to implementation include unfamiliarity with the CPGs and financial concerns. Effective interventions to improve adherence are possible, especially when they are readily accessible at the point of care delivery. Computerized clinical support systems show particular promise. The authors introduce the clinical decision making modules (CDMMs) of the Thyroid Cancer Care Collaborative, a thyroid cancer‐specific electronic health record. These computer‐based modules can assist clinicians with implementation of these recommendations in clinical practice.ConclusionComputer‐based support systems can help clinicians understand and adopt the thyroid cancer CPGs. By integrating patient characteristics and guidelines at the point of care delivery, the CDMMs can improve adherence to the guidelines and help clinicians provide high‐quality, evidence‐based, and individualized patient care in the management of differentiated thyroid cancer. Laryngoscope, 126:2640–2645, 2016
Optimal bovine collagen concentration to achieve tracheal epithelial coverage of collagen sponges
Authors: Suzuki Ryo , Nakamura Ryosuke , Nakaegawa Yuta , Nomoto Yukio , Fujimoto Ichiro , Semura Kayoko , Hazama Akihiro , Omori Koichi 
Published: April 2016

Objectives/HypothesisArtificial tracheas prepared using a collagen sponge and polypropylene mesh have been implanted in patients who received tracheal resections, but epithelialization in the reconstructed area is slow. We determined the optimal bovine atelocollagen concentration necessary for the rapid and complete tracheal epithelial coverage of collagen sponge implants.Study DesignPreliminary animal experiment.MethodsCollagen sponges were prepared using lyophilizing 0.5%, 0.7%, and 1.0% atelocollagen solutions (0.5%, 0.7%, and 1.0% sponges) and were analyzed using scanning electron microscopy. Partial tracheal defects were prepared in rabbits and reconstructed using sponges. Epithelial regeneration in the reconstructed area was evaluated by endoscopic, histological, and scanning electron microscope analyses.ResultsAll sponges had a membranous structural framework, and numerous fibrous structures filled the spaces within the framework in the 0.5% sponges. The membranous structure in the 0.7% sponges branched at many points, and intermembrane spaces were frequently observed. Conversely, the membranous structure in the 1.0% sponges was relatively continuous, thick, and closely arranged. Two weeks after implantation, tracheal defects were entirely covered with epithelium in two of the four and three of the four of the 0.5% and 0.7% sponge‐implanted rabbits, respectively. The collagen sponges remained exposed to the tracheal lumen in four of the four rabbits in the 1.0% sponge group. Ciliogenesis in the center of the epithelialized region was detected only in the 0.7% sponge group.ConclusionCollagen sponges prepared from various concentrations of bovine atelocollagen have different structures. Complete epithelial coverage was achieved in more rabbits implanted with sponges prepared using the 0.7% bovine atelocollagen solution than in those implanted with sponges prepared from the 0.5% and 1.0% solutions.Level of EvidenceNA Laryngoscope, 126:E396–E403, 2016
Supramaximal neurostimulation with laryngeal palpation to predict postoperative vocal fold mobility
Authors: Cha Wonjae , Cho Ilyoung , Jang Jeon Yeob , Cho Jae‐Keun , Wang Soo‐Geun , Park Ji‐Hwan 
Published: April 2016

Objectives/HypothesisRecurrent laryngeal nerve (RLN) injury during thyroidectomy or parathyroidectomy is a challenging issue and causes significant morbidity. We adopted the supramaximal stimulation protocol for neurostimulation with laryngeal palpation (NSLP) and tried to evaluate the predictive values of supramaximal NSLP for immediate postoperative vocal fold (VF) mobility.Study DesignProspective cohort study.MethodsProspectively, 293 patients who underwent thyroid and parathyroid surgery and 542 RLNs at risk were enrolled in this study. During NSLP, the current was escalated to 3 mA until definite laryngeal twitch was observed. Immediate postoperative VF mobility was evaluated using flexible laryngoscopy.ResultsDiagnostic accuracy of NSLP is calculated according to cutoff values of minimal current intensity (1 mA, 1.5 mA, and 2 mA). At 2mA, sensitivity was 81.82%, specificity 100%, positive predictive value 100%, and negative predictive value 99.62%.ConclusionsSupramaximal NSLP might be a simple and reliable method to predict immediate postoperative VF mobility in open thyroid and parathyroid surgeries when intraoperative neuromonitoring is unavailable.Level of Evidence4. Laryngoscope, 126:2863–2868, 2016
Complication rates of open surgical versus percutaneous tracheostomy in critically ill patients
Authors: Johnson‐Obaseki Stephanie , Veljkovic Andrea , Javidnia Hedyeh 
Published: April 2016

BackgroundIn the setting of critical care, the most common indications for tracheostomy include: prolonged intubation, to facilitate weaning from mechanical ventilation, and for pulmonary toileting. In this setting, tracheostomy can be performed either via open surgical or percutaneous technique. Advantages for percutaneous dilatational tracheostomy (PDT) include: simplicity, smaller incision, less tissue trauma, lower incidence of wound infection, lower incidence of peristomal bleeding, decreased morbidity from patient transfer, and cost‐effectiveness. Despite many studies comparing surgical tracheostomy (ST) versus PDT, there remains no consensus on which of these techniques minimizes complications in critically ill patients.PurposeTo provide an updated meta‐analysis to answer the following question: Is there a difference in complication rates between ST and PDT in the setting of critically ill patients? Our secondary outcome of interest was to examine the difference in procedure time in the ST versus PDT groups.MethodsWe conducted a literature search using the following databases: Ovid MEDLINE, Embase, Google Scholar, and Cochrane Database of Systematic Reviews. Studies from 1985 until October 2014 published in French or English languages in peer‐reviewed journals were included.ResultsWith regard to rates of mortality, intraoperative hemorrhage, and postoperative hemorrhage, there was no statistically significant difference between the two techniques. Evaluation of infections rates and operative time, however, revealed a statistically significant difference, favoring PDT over ST.ConclusionIn critically ill patients, PDT appears to be a safe and efficient alternative to open ST.Level of EvidenceNA Laryngoscope, 126:2459–2467, 2016
Flavonoid glycosides from Persea caerulea. Unraveling their interactions with SDS‐micelles through matrix‐assisted DOSY, PGSE, mass spectrometry, and NOESY
Authors: Álvarez Juan M. , Raya‐Barón Álvaro , Nieto Pedro M. , Cuca Luis E. , Carrasco‐Pancorbo Alegría , Fernández‐Gutiérrez Alberto , Fernández Ignacio 
Published: April 2016

AbstractTwo flavonoid glycosides derived from rhamnopyranoside (1) and arabinofuranoside (2) have been isolated from leaves of Persea caerulea for the first time. The structures of 1 and 2 have been established by 1H NMR, 13C NMR, and IR spectroscopy, together with LC–ESI–TOF and LC–ESI–IT MS spectrometry. From the MS and MS/MS data, the molecular weights of the intact molecules as well as those of quercetin and kaempferol together with their sugar moieties were deduced. The NMR data provided information on the identity of the compounds, as well as the α and β configurations and the position of the glycosides on quercetin and kaempferol. We have also explored the application of sodium dodecyl sulfate (SDS) normal micelles in binary aqueous solution, at a range of concentrations, to the diffusion resolution of these two glycosides, by the application of matrix‐assisted diffusion ordered spectroscopy (DOSY) and pulse field gradient spin echo (PGSE) methodologies, showing that SDS micelles offer a significant resolution which can, in part, be rationalized in terms of differing degrees of hydrophobicity, amphiphilicity, and steric effects. In addition, intra‐residue and inter‐residue proton–proton distances using nuclear Overhauser effect build‐up curves were used to elucidate the conformational preferences of these two flavonoid glycosides when interacting with the micelles. By the combination of both diffusion and nuclear Overhauser spectroscopy techniques, the average location site of kaempferol and quercetin glycosides has been postulated, with the former exhibiting a clear insertion into the interior of the SDS‐micelle, whereas the latter is placed closer to the surface. Copyright © 2016 John Wiley & Sons, Ltd.
‘It is a Big Thing’: Exploring the Impact of Osteoarthritis from the Perspective of Adults Caring for Parents – The Sandwich Generation
Authors: Barker Karen L. , Minns Lowe Catherine J. , Toye Francine 
Published: April 2016

AbstractPurposeThe aim of the present study was to explore the experiences and impact of caring for an individual with severe osteoarthritis (OA) from the perspective of adult children, looking at the relationship between adult children caring for parents with this condition and the tensions of the ‘sandwich generation’.MethodsA mixed qualitative approach, combining focus groups and individual semi‐structured interviews was used. In total, 36 participants were purposively sampled and discussed the impact of caring for a parent with OA. Data analysis was based upon interpretative phenomenological analysis.ResultsFindings reported the impact and complexity of caring for a parent with OA. We present three themes related to the work of caring for a relative with this condition: (i) the physical and emotional work of caring; (ii) changes in reciprocal family roles; (iii) the imbalance in caring roles within the family.ConclusionsParticipants described the significant and extensive impact on their lives of caring for a parent with long‐term OA, particularly when faced with the pressures of caring for their own children as well. Copyright © 2016 The Authors Musculoskeletal Care Published by John Wiley & Sons Ltd
Growth of zinc sulfide by mist chemical vapor deposition depending on mist size and thermal conditions in susceptor
Authors: Uno Kazuyuki , Yamasaki Yuichiro , Gu Ping , Tanaka Ichiro 
Published: April 2016

AbstractZinc sulfide (ZnS) films were successfully grown by using mist chemical vapor deposition (mist‐CVD) with fine‐channel susceptor made of quartz. For the investigation of growth dynamics, two types of mist generators and two types of susceptors were prepared and distribu¬tion profiles of film thickness were examined.Thermal efficiency of susceptor depends on heat¬penetration efficiency and line‐velocity of the mists, which are influenced by the fine‐channel gap‐height of the susceptors. Using larger size of mists requires higher thermal efficiency in susceptor to obtain a flat thickness distribution. This result indicates that the optimization of the mist size and the susceptor structure is important for the film uniformity of grown films by the mist‐CVD. (© 2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)