bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conditional Deletion of Fgfr3 in Chondrocytes leads to Osteoarthritis-like Defects in Temporomandibular Joint of Adult Mice
Authors: Zhou Siru , Xie Yangli , Li Wei , Huang Junlan , Wang Zuqiang , Tang Junzhou , Xu Wei , Sun Xianding , Tan Qiaoyan , Huang Shuo , Luo Fengtao , Xu Meng , Wang Jun , Wu Tingting , chen Liang , Chen Hangang , Su Nan , Du Xiaolan , Shen Yue , Chen Lin 
Published: April 2016

AbstractOsteoarthritis (OA) in the temporomandibular joint (TMJ) is a common degenerative disease in adult, which is characterized by progressive destruction of the articular cartilage. To investigate the role of FGFR3 in the homeostasis of TMJ cartilage during adult stage, we generated Fgfr3f/f; Col2a1-CreERT2 (Fgfr3 cKO) mice, in which Fgfr3 was deleted in chondrocytes at 2 months of age. OA-like defects were observed in Fgfr3 cKO TMJ cartilage. Immunohistochemical staining and quantitative real-time PCR analyses revealed a significant increase in expressions of COL10, MMP13 and AMAMTS5. In addition, there was a sharp increase in chondrocyte apoptosis at the Fgfr3 cKO articular surface, which was accompanied by a down-regulation of lubricin expression. Importantly, the expressions of RUNX2 and Indian hedgehog (IHH) were up-regulated in Fgfr3 cKO TMJ. Primary Fgfr3 cKO chondrocytes were treated with IHH signaling inhibitor, which significantly reduced expressions of Runx2, Col10, Mmp13 and Adamts5. Furthermore, the IHH signaling inhibitor partially alleviated OA-like defects in the TMJ of Fgfr3 cKO mice, including restoration of lubricin expression and improvement of the integrity of the articular surface. In conclusion, our study proposes that FGFR3/IHH signaling pathway plays a critical role in maintaining the homeostasis of TMJ articular cartilage during adult stage.
Behavioral and neural correlates of self-referential processing deficits in bipolar disorder
Authors: Zhao Yanli , Luo Wenbo , Chen Jingxu , Zhang Dandan , Zhang Ligang , Xiao Chunling , Fan Fengmei , Zhu Xiaolin , Fan Hongzhen , Tan Shuping 
Published: April 2016

AbstractSelf-referential processing is a core component of social cognition. However, few studies have focused on whether self-referential processing deficits present in bipolar disorder. The current study combined a high-time-resolution event-related potential (ERP) technique with the self-referential memory (SRM) task to evaluate self-referential processing in 23 bipolar patients and 27 healthy controls. All subjects showed a reliable SRM effect, but the bipolar group had poorer recognition scores for the self- and other-referential conditions. The bipolar group presented with smaller voltages in both the self- and other-referential conditions for the N1 (150–220 ms) and the P2 components (130–320 ms) but larger voltages in the positive slow wave (600–1600 ms) component. Larger P3 amplitudes were elicited in the self-referential condition compared with the other-referential condition in controls but not in bipolar patients. Additionally, non-psychotic bipolar patients had a comparative normal SRM effect which was abolished in psychotic bipolar patients; non-psychotic bipolar patients had larger amplitudes of the positive slow wave than the normal controls, whereas it was not differed between psychotic bipolar patients and the healthy subjects. The present study suggests that self- and other-referential processing is impaired in bipolar patients and the deficits may be more pronounced in psychotic bipolar patients.
Proton mediated control of biochemical reactions with bioelectronic pH modulation
Authors: Deng Yingxin , Miyake Takeo , Keene Scott , Josberger Erik E. , Rolandi Marco 
Published: April 2016

AbstractIn Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. The transducer also controls bioluminescence from firefly luciferase by affecting solution pH.
Differential coding of uncertain reward in rat insular and orbitofrontal cortex
Authors: Jo Suhyun , Jung Min Whan 
Published: April 2016

AbstractAnterior insular and orbitofrontal cortex (AIC and OFC, respectively) are known to play important roles in decision making under risk. However, risk-related AIC neural activity has not been investigated and it is controversial whether the rodent OFC conveys genuine risk signals. To address these issues, we examined AIC and OFC neuronal activity in rats responding to five distinct auditory cues predicting water reward with different probabilities. Both structures conveyed significant neural signals for reward, value and risk, with value and risk signals conjunctively coded. However, value signals were stronger and appeared earlier in the OFC, and many risk-coding OFC neurons responded only to the cue predicting certain (100%) reward. Also, AIC neurons tended to increase their activity for a prolonged time following a negative outcome and according to previously expected value. These results show that both the AIC and OFC convey neural signals related to reward uncertainty, but in different ways. The OFC might play an important role in encoding certain reward-biased, risk-modulated subjective value, whereas the AIC might convey prolonged negative outcome and disappointment signals.
An insight in magnetic field enhanced zero-valent iron/H2O2 Fenton-like systems: Critical role and evolution of the pristine iron oxides layer
Authors: Xiang Wei , Zhang Beiping , Zhou Tao , Wu Xiaohui , Mao Juan 
Published: April 2016

AbstractThis study demonstrated the synergistic degradation of 4-chlorophenol (4-CP) achieved in a magnetic field (MF) enhanced zero-valent iron (ZVI)/H2O2 Fenton-like (FL) system and revealed an interesting correlative dependence relationship between MF and the pristine iron oxides layer (FexOy) on ZVI particles. First, a comparative investigation between the FL and MF-FL systems was conducted under different experimental conditions. The MF-FL system could suppress the duration of initial lag degradation phase one order of magnitude in addition of the significant enhancement in overall 4-CP degradation. Monitoring of intermediates/products indicated that MF would just accelerate the Fenton reactions to produce hydroxyl radical more rapidly. Evolutions of simultaneously released dissolved iron species suggested that MF would not only improve mass-transfer of the initial heterogeneous reactions, but also modify the pristine ZVI surface. Characterizations of the specific prepared ZVI samples evidenced that MF would induce a special evolution mechanism of the ZVI particles surface depending on the existence of FexOy layer. It comprised of an initial rapid point dissolution of FexOy and a following pitting corrosion of the exposed Fe0 reactive sites, finally leading to appearance of a particular rugged surface topography with numerous adjacent Fe0 pits and FexOy tubercles.
Association between TP53 Arg72Pro polymorphism and leukemia risk: a meta-analysis of 14 case-control studies
Authors: Tian Xin , Dai Shundong , Sun Jing , Jiang Shenyi , Jiang Youhong 
Published: April 2016

AbstractThe relationship between the TP53 Arg72Pro polymorphism (rs1042522) and the risk of leukemia remains controversial. Consequently, we performed a meta-analysis to accurately evaluate the association between TP53 Arg72Pro polymorphism and leukemia risk. A comprehensive search was conducted to find all eligible studies of TP53 Arg72Pro polymorphism and leukemia risk. Fourteen case-control studies, with 2,506 cases and 4,386 controls, were selected for analysis. The overall data failed to indicate a significant association between TP53 Arg72Pro polymorphism and the risk of leukemia (C vs. G: OR = 1.09, 95% CI = 0.93–1.26; CC vs. GC + GG: OR = 1.23, 95% CI = 0.96–1.57). In a subgroup analysis of clinical types, an increased risk was observed in the acute lymphocytic leukemia (ALL) subgroup (CC vs. GC + GG: OR = 1.73; 95% CI = 1.07–2.81) but not in the acute myeloid leukemia (AML) subgroup. In the subgroup analysis, no significant associations with ethnicity and the source of the controls were observed. In conclusion, the results suggest that there is no association between TP53 Arg72Pro polymorphism and the risk of leukemia, but the CC genotype may increase the risk of ALL TP53 Arg72Pro polymorphism CC genotype may increase the risk of ALL but is not associated with AML. Further large-scale, well-designed studies are needed to confirm our results.
A deformation energy-based model for predicting nucleosome dyads and occupancy
Authors: Liu Guoqing , Xing Yongqiang , Zhao Hongyu , Wang Jianying , Shang Yu , Cai Lu 
Published: April 2016

AbstractNucleosome plays an essential role in various cellular processes, such as DNA replication, recombination and transcription. Hence, it is important to decode the mechanism of nucleosome positioning and identify nucleosome positions in the genome. In this paper, we present a model for predicting nucleosome positioning based on DNA deformation, in which both bending and shearing of the nucleosomal DNA are considered. The model successfully predicted the dyad positions of nucleosomes assembledin vitroand thein vitromap of nucleosomes inSaccharomyces cerevisiae. Applying the model toCaenorhabditis elegansandDrosophila melanogaster, we achieved satisfactory results. Our data also show that shearing energy of nucleosomal DNA outperforms bending energy in nucleosome occupancy prediction and the ability to predict nucleosome dyad positions is attributed to bending energy that is associated with rotational positioning of nucleosomes.
Electronic and magnetic properties of Co doped MoS2 monolayer
Authors: Wang Yiren , Li Sean , Yi Jiabao 
Published: April 2016

AbstractFirst principle calculations are employed to calculate the electronic and magnetic properties of Co doped MoS2 by considering a variety of defects including all the possible defect complexes. The results indicate that pristine MoS2 is nonmagnetic. The materials with the existence of S vacancy or Mo vacancy alone are non-magnetic either. Further calculation demonstrates that Co substitution at Mo site leads to spin polarized state. Two substitutional CoMo defects tend to cluster and result in the non-magnetic behaviour. However, the existence of Mo vacancies leads to uniform distribution of Co dopants and it is energy favourable with ferromagnetic coupling, resulting in an intrinsic diluted magnetic semiconductor.
Bioinspired Immobilization of Glycerol Dehydrogenase by Metal Ion-Chelated Polyethyleneimines as Artificial Polypeptides
Authors: Zhang Yonghui , Ren Hong , Wang Yali , Chen Kainan , Fang Baishan , Wang Shizhen 
Published: April 2016

AbstractIn this study, a novel, simple and generally applicable strategy for multimeric oxidoreductase immobilization with multi-levels interactions was developed and involved activity and stability enhancements. Linear polyethyleneimines (PEIs) are flexible cationic polymers with molecular weights that span a wide range and are suitable biomimic polypeptides for biocompatible frameworks for enzyme immobilization. Metal ion-chelated linear PEIs were applied as a heterofunctional framework for glycerol dehydrogenase (GDH) immobilization by hydrogen bonds, electrostatic forces and coordination bonds interactions. Nanoparticles with diameters from 250–650 nm were prepared that exhibited a 1.4-fold enhancement catalytic efficiency. Importantly, the half-life of the immobilized GDH was enhanced by 5.6-folds in aqueous phase at 85 °C. A mechanistic illustration of the formation of multi-level interactions in the PEI-metal-GDH complex was proposed based on morphological and functional studies of the immobilized enzyme. This generally applicable strategy offers a potential technique for multimeric enzyme immobilization with the advantages of low cost, easy operation, high activity reservation and high stability.
Mouse Sox17 haploinsufficiency leads to female subfertility due to impaired implantation
Authors: Hirate Yoshikazu , Suzuki Hitomi , Kawasumi Miyuri , Takase Hinako M. , Igarashi Hitomi , Naquet Philippe , Kanai Yoshiakira , Kanai-Azuma Masami 
Published: April 2016

AbstractEmbryonic implantation comprises a dynamic and complicated series of events, which takes place only when the maternal uterine endometrium is in a receptive state. Blastocysts reaching the uterus communicate with the uterine endometrium to implant within a narrow time window. Interplay among various signalling molecules and transcription factors under the control of ovarian hormones is necessary for successful establishment of pregnancy. However, the molecular mechanisms that allow embryonic implantation in the receptive endometrium are still largely unknown. Here, we show that Sry-related HMG box gene-17 (Sox17) heterozygous mutant female mice exhibit subfertility due to implantation failure. Sox17 was expressed in the oviduct, uterine luminal epithelium, and blood vessels. Sox17 heterozygosity caused no appreciable defects in ovulation, fertilisation, blastocyst formation, and gross morphology of the oviduct and uterus. Another group F Sox transcription factor, Sox7, was also expressed in the uterine luminal and glandular epithelium relatively weakly. Despite uterine Sox7 expression, a significant reduction in the number of implantation sites was observed in Sox17 heterozygous mutant females due to haploinsufficiency. Our findings revealed a novel role of Sox17 in uterine receptivity to embryo implantation.
In vivo imaging demonstrates dendritic spine stabilization by SynCAM 1
Authors: Körber Nils , Stein Valentin 
Published: April 2016

AbstractFormation and stability of synapses are required for proper brain function. While it is well established that synaptic adhesion molecules are important regulators of synapse formation, their specific role during different phases of synapse development remains unclear. To investigate the function of the synaptic cell adhesion molecule SynCAM 1 in the formation, stability and maintenance of spines we used 2-photon in vivo imaging to follow individual spines over a long period of time. In SynCAM 1 knockout mice the survival rate of existing spines was reduced and fewer filopodia-like structures were converted into stable spines. SynCAM 1flag overexpression resulted in more stable spines and fewer filopodia-like structures. When SynCAM 1flag overexpression is turned on the spine density rapidly increases within a few days. Interestingly, the spine density stayed at an elevated level when SynCAM 1flag overexpression was turned off. Our data indicate that the SynCAM 1 induced altered spine density is not caused by the formation of newly emerging protrusions, instead SynCAM 1 stabilizes nascent synaptic contacts which promotes their maturation. Concomitant with the synaptic stabilization, SynCAM 1 generally prolongs the lifetime of spines. In summary, we demonstrate that SynCAM 1 is a key regulator of spine stability.
Robust and tunable itinerant ferromagnetism at the silicon surface of the antiferromagnet GdRh2Si2
Authors: Güttler M. , Generalov A. , Otrokov M. M. , Kummer K. , Kliemt K. , Fedorov A. , Chikina A. , Danzenbächer S. , Schulz S. , Chulkov E. V. , Koroteev Yu. M. , Caroca-Canales N. , Shi M. , Radovic M. , Geibel C. , Laubschat C. , Dudin P. , Kim T. K. , Hoesch M. , Krellner C. , Vyalikh D. V. 
Published: April 2016

AbstractSpin-polarized two-dimensional electron states (2DESs) at surfaces and interfaces of magnetically active materials attract immense interest because of the idea of exploiting fermion spins rather than charge in next generation electronics. Applying angle-resolved photoelectron spectroscopy, we show that the silicon surface of GdRh2Si2bears two distinct 2DESs, one being a Shockley surface state and the other a Dirac surface resonance. Both are subject to strong exchange interaction with the ordered 4f-moments lying underneath the Si-Rh-Si trilayer. The spin degeneracy of the Shockley state breaks down below ~90 K and the splitting of the resulting subbands saturates upon cooling at values as high as ~185 meV. The spin splitting of the Dirac state becomes clearly visible around ~60 K, reaching a maximum of ~70 meV. An abrupt increase of surface magnetization at around the same temperature suggests that the Dirac state contributes significantly to the magnetic properties at the Si surface. We also show the possibility to tune the properties of 2DESs by depositing alkali metal atoms. The unique temperature-dependent ferromagnetic properties of the Si-terminated surface in GdRh2Si2could be exploited when combined with functional adlayers deposited on top for which novel phenomena related to magnetism can be anticipated.
Collagen Microspheres: A Three-Dimensional Culture System for Notochordal Cells
Authors: Yuan Minting , Yeung Chiu Wai , Au Tiffany , Chan Danny , Cheah Kathryn S. E. , Chan Barbara P. 
Published: April 2016

Introduction The notochord plays a crucial role in the formation and patterning of axial skeleton. Notochordal cells (NCCs) have drawn increasing attentions from developmental biology, stem cells research and tissue engineering in these years as they are important in development and maintenance of NP. However, it is difficult to maintain their survival and phenotype in 2D culture for long term, making it difficult to understand their fate and functional maintenance during intervertebral disc development. This project is to develop a better culture system that is able to maintain the survival and phenotypes of NCCs. Materials and Methods Foxa2mNE–Cre/Z/EG heterozygous embryos were developed and NCCs could be identified EGFP signal. NCC isolated with or without FACS-sorting, notochord segments at the anterior, trunk and posterior regions, and whole notochord pulled out from embryo were microencapsulated respectively in type I collagen microspheres and cultured for up to 1 month before characterization for survival and phenotype maintenance. Results EGFP signal of sorted cells could be maintained after 4 weeks. The EGFP positive cells in collagen microspheres showed colocalization with foxa2 and brachyury, which were considered as markers of NCCs, indicated that they were still NCCs. However, areas with these merged were very rare. EGFP signal of unsorted cells could be maintained and clusters of EGFP positive cells were showed during 4-weeks culture. When the notochord segments were cultured in collagen microspheres, clusters of round EGFP positive cells co-expressing with major NCC markers were found after 1 month, suggesting that NCC phenotype was maintained. Moreover, the number of EGFP positive cells was increased during culture. In the other hand, when the notochord was cultured in collagen microsphere, it started pinching and the pinching region seemed growing at day 28. Conclusion Type I collagen microspheres present a potential culture system for increasing cell number with EGFP positive signal for at least 4 weeks. NCCs entrapped in type I collagen microspheres could maintained the NCCs phenotype. Comparing with FACS-sorted NCCs, there was increasing number of NCCs in unsorted group, segment culture and whole notochord culture, suggesting that the presence of niche cells, extracellular matrix, and native configuration of notochord may be important to support NCCs growth and phenotype maintenance. Acknowledgments This work was supported by AoE (AoE/M-04/04), TBRS (T12–708/12-N) and AOSPN (SRN_2011_14). Disclosures: None
The Species-Specific Regenerative Effect of Notochordal Cell-conditioned Medium on Degenerated Human Nucleus Pulposus Cells
Authors: Bach Frances , de Vries Stefan , van Heel Ferdi , Krouwels Anita , Creemers Laura , Ito Keita , Meij Björn , Tryfonidou Marianna 
Published: April 2016

Introduction During human intervertebral disc (IVD) degeneration, the main cell type shifts from notochordal cells (NCs) to nucleus pulposus cells (NPCs). NCs secrete factors with regenerative potential, making them an interesting focus for regenerative strategies. Research into these strategies employs non-human donors due to easy availability of their NC-rich nucleus pulposus (NP) tissue. To determine the feasibility of translating these strategies toward clinical application, this study aimed to delineate whether NC-secreted factors of different species have a cross-species regenerative effect on degenerated human NPCs. Material and Methods Healthy human, porcine and canine NC-rich NP tissues (derived from Thompson grade I IVDs) were compared by histology, collagen, DNA and glycosaminoglycan (GAG) content per mg wet tissue weight. NP tissue of each species was cultured for 4 days and NC-conditioned medium (NCCM) was collected. Human NPC micro-aggregates from degenerated IVDs (Thompson grade III) were cultured for 28 days in human, canine or porcine NCCM. GAG content (corrected for DNA) was determined, and Safranin O/Fast Green staining, collagen type I, II and X IHC and gene expression profiling were performed. Results Canine and porcine NPs were richer in NCs than human NPs. Human NPs contained the highest collagen content, whereas the DNA and GAG content of canine NPs was significantly higher than human or porcine NPs. NCCM from all species significantly increased the DNA and GAG content of the human NPC micro-aggregates. Porcine and canine NCCM were significantly more potent than human NCCM in inducing GAG deposition, whereas only human NCCM induced collagen type II production. Conclusion Secreted factors from human, canine and porcine NC-rich NPs exerted regenerative effects on human NPCs, indicating a cross-species effect. The species-specific NP properties appear to influence the regenerative capacity of NCCM on human NPCs. Bioactive compound(s) with treatment potential for human IVD degeneration are present in NCCM of different species, implying that strategies based on NC-technology employing canine or porcine models have potential for successful translation into humans. Acknowledgments This work was supported by an AOSpine Research Network grant (SRN2011_11) and the Dutch Arthritis Foundation (LLP22).