bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized trial of iron isomaltoside versus iron sucrose in patients with iron deficiency anemia
Authors: Derman Richard , Roman Eloy , Modiano Manuel R. , Achebe Maureen M. , Thomsen Lars L. , Auerbach Michael 
Published: January 2017

AbstractIron deficiency anemia (IDA) is common in many chronic diseases, and intravenous (IV) iron offers a rapid and efficient iron correction. This trial compared the efficacy and safety of iron isomaltoside (also known as ferric derisomaltose) and iron sucrose in patients with IDA who were intolerant of, or unresponsive to, oral iron. The trial was an open‐label, comparative, multi‐center trial. Five hundred and eleven patients with IDA from different causes were randomized 2:1 to iron isomaltoside or iron sucrose and followed for 5 weeks. The cumulative dose of iron isomaltoside was based on body weight and hemoglobin (Hb), administered as either a 1000 mg infusion over more than 15 minutes or 500 mg injection over 2 minutes. The cumulative dose of iron sucrose was calculated according to Ganzoni and administered as repeated 200 mg infusions over 30 minutes. The mean cumulative dose of iron isomaltoside was 1640.2 (standard deviation (SD): 357.6) mg and of iron sucrose 1127.9 (SD: 343.3) mg. The primary endpoint was the proportion of patients with a Hb increase ≥2 g/dL from baseline at any time between weeks 1‐5. Both non‐inferiority and superiority were confirmed for the primary endpoint, and a shorter time to Hb increase ≥2 g/dL was observed with iron isomaltoside. For all biochemical efficacy parameters, faster and/or greater improvements were found with iron isomaltoside. Both treatments were well tolerated; 0.6% experienced a serious adverse drug reaction. Iron isomaltoside was more effective than iron sucrose in achieving a rapid improvement in Hb. Furthermore, iron isomaltoside has an advantage over iron sucrose in allowing higher cumulative dosing in fewer administrations. Both treatments were well tolerated in a broad population with IDA.
Ten-year report on axillary recurrence after negative sentinel node biopsy for breast cancer from the Swedish Multicentre Cohort Study
Authors: de Boniface J , Frisell J , Bergkvist L , Andersson Y 
Published: January 2017

Abstract

Background
The omission of axillary lymph node dissection (ALND) in patients with breast cancer with a negative finding on sentinel node biopsy (SNB) has reduced arm morbidity substantially. Early follow-up reports have shown the rate of axillary recurrence to be significantly lower than expected, with a median false-negative rate of 7 per cent for SNB. Long-term follow-up is needed as recurrences may develop late.

Methods
The Swedish Multicentre Cohort Study included 3518 women with breast cancer and a clinically negative axilla, in whom SNB was planned. ALND was performed only in patients with sentinel node metastasis. Twenty-six centres contributed to enrolment between September 2000 and January 2004. The primary endpoint was the axillary recurrence rate and the secondary endpoint was breast cancer-specific survival, calculated using Kaplan–Meier survival estimates.

Results
Some 2216 sentinel node-negative patients with 2237 breast cancers were analysed. The median follow-up time was 126 (range 0–174) months. Isolated axillary recurrence was found in 35 patients (1·6 per cent). High histological grade and multifocal tumours were risk factors for axillary recurrence, whereas the removal of more than two sentinel nodes decreased the risk. Fourteen (40 per cent) of 35 patients died as a consequence of axillary recurrence.

Conclusion
The risk of axillary recurrence remains lower than expected after a negative finding on SNB at 10-year follow-up. Axillary recurrences may occur long after primary surgery, and lead to a significant risk of breast cancer death.
Prognostic value of carbohydrate antigen 19-9 in patients undergoing resection of biliary tract cancer
Authors: Yamashita S , Passot G , Aloia T A , Chun Y S , Javle M , Lee J E , Vauthey J-N , Conrad C 
Published: January 2017

Abstract

Background
The clinical significance of abnormally high levels of carbohydrate antigen (CA) 19-9 after resection of biliary tract cancer (BTC) is not well established. The aim of this study was to determine the prognostic value of CA19-9 normalization in patients undergoing resection of BTC with curative intent.

Methods
Patients with BTC undergoing resection with curative intent (1996–2015) were divided into those with normal preoperative CA19-9 level (normal CA19-9 group), those with an abnormally high preoperative CA19-9 level (over 37 units/ml) and normal postoperative CA19-9 level (normalization group), and those with an abnormally high preoperative CA19-9 level and abnormally high postoperative CA19-9 level (non-normalization group). Overall survival (OS) was analysed and predictors of OS were determined.

Results
The normal CA19-9 group (180 patients) and normalization group (74) had better OS than the non-normalization group (58) (3-year OS rate 70·4, 73 and 31 per cent respectively; both P &lt; 0·001). The normal CA19-9 and normalization groups had equivalent OS (P = 0·880). On multivariable analysis, factors associated with worse OS were lymph node metastases (hazard ratio (HR) 1·78; P = 0·014) and abnormally high postoperative CA19-9 level (HR 3·16; P &lt; 0·001). In the normalization group, OS did not differ after R0versus R1 resection (3-year OS rate 69 versus 62 per cent respectively; P = 0·372); in the non-normalization group, patients with R1 resection had worse OS (3-year OS rate 36 and 20 per cent for R0 and R1 respectively; P = 0·032).

Conclusion
Non-normalization of CA19-9 level after resection of BTC with curative intent was associated with worse OS. R1 resection was associated with a particularly poor prognosis when CA19-9 levels did not normalize.
Apoptotic effects of bovine apo‐lactoferrin on HeLa tumor cells
Authors: Luzi Carla , Brisdelli Fabrizia , Iorio Roberto , Bozzi Argante , Carnicelli Veronica , Di Giulio Antonio , Lizzi Anna Rita 
Published: January 2017

Lactoferrin (Lf), a cationic iron‐binding glycoprotein of 80 kDa present in body secretions, is known as a compound with marked antimicrobial activity. In the present study, the apoptotic effect of iron‐free bovine lactoferrin (apo‐bLf) on human epithelial cancer (HeLa) cells was examined in association with reactive oxygen species and glutathione (GSH) levels. Apoptotic effect of iron‐free bovine lactoferrin inhibited the growth of HeLa cells after 48 hours of treatment while the diferric‐bLf was ineffective in the concentration range tested (from 1 to 12.5 μM). Western blot analysis showed that key apoptotic regulators including Bax, Bcl‐2, Sirt1, Mcl‐1, and PARP‐1 were modulated by 1.25 μM of apo‐bLf. In the same cell line, apo‐bLf induced apoptosis together with poly (ADP‐ribose) polymerase cleavage, caspase activation, and a significant drop of NAD+. In addition, apo‐bLf–treated HeLa cells showed a marked increase of reactive oxygen species level and a significant GSH depletion. On the whole, apo‐bLf triggered apoptosis of HeLa cells upon oxygen radicals burst and GSH decrease.
BARD1 nonsense variant c.1921C>T in a patient with recurrent breast cancer
Authors: Gass Jennifer , Tatro Madeline , Blackburn Patrick , Hines Stephanie , Atwal Paldeep S. 
Published: January 2017

Key Clinical MessageOne of the strongest risk factors predisposing patients to breast cancer is a positive family history. In our study, we describe a patient diagnosed with multiple breast cancer tumors. Genetic analysis revealed a pathogenic variant in BARD1, which is associated with an increased risk of developing certain types of cancer.
Chemoselective Synthesis of Dithioacetals from Bio‐aldehydes with Zeolites under Ambient and Solvent‐free Conditions
Authors: Li Hu , Yang Tingting , Riisager Anders , Saravanamurugan Shunmugavel , Yang Song 
Published: January 2017

AbstractDithioacetals are an important class of versatile compounds extensively applied in pharmaceuticals, separations, electrochemistry, and organic synthesis, but few heterogeneous catalytic systems are reported to be generally applicable for their synthesis from a wide range of substrates. A series of commercial and modified zeolites are excellent catalysts for thioacetalization of different thiols with carbonyl compounds, including biomass‐derived aldehydes, at room temperature under solvent‐free conditions. A near quantitative yield of dithioacetal was obtained over H‐beta(19) at room temperature with a low catalyst to substrate ratio of 1:19, and a method to follow the reaction progress by ex situ UV/Vis absorption analysis was demonstrated. Recycling experiments with H‐beta(19) in five consecutive runs resulted in slight loss of activity, but the original activity could be fully restored after calcination at 550 °C. The results and physicochemical properties of the zeolites revealed that relatively large pores and moderate acidity with an appropriate distribution of Brønsted/Lewis acid sites contributed to the pronounced performance in the dithioacetal formation.
Influence of the Dithiolate Bridge on the Oxidative Processes of Diiron Models Related to the Active Site of [FeFe] Hydrogenases
Authors: Arrigoni Federica , Mohamed Bouh Salma , De Gioia Luca , Elleouet Catherine , Pétillon François Y. , Schollhammer Philippe , Zampella Giuseppe 
Published: January 2017

AbstractElectrochemical studies of [Fe2(CO)4(κ2‐dmpe)(μ‐dithiolate)] (dithiolate=adtBn, pdt) and density functional theory (DFT) calculations reveal the striking influence of an amine functionality in the dithiolate bridge on their oxidative properties. [Fe2(CO)4(κ2‐dmpe)(μ‐adtBn)] (1) undergoes two one‐electron oxidation steps, with the first being partially reversible and the second irreversible. When the adtBn bridge is replaced with pdt, a shift of 60 mV towards more positive potentials is observed for the first oxidation whereas 290 mV separate the oxidation potentials of the two cations. Under CO, oxidation of azadithiolate compound 1 occurs according to an ECE process whereas an EC mechanism takes place for the propanedithiolate species 2. The dication species [1‐CO]2+ resulting from the two‐electron oxidation of 1 has been spectroscopically and structurally characterized. The molecular details underlying the reactivity of oxidized species have been explored by DFT calculations. The differences in the behaviors of 1 and 2 are mainly due to the presence, or not, of favored interactions between the dithiolate bridge and the diiron site depending on the redox states, FeIFeII or FeIIFeII, of the complexes.
Synthesis of Polydopamine‐Like Nanocapsules via Removal of a Sacrificial Mesoporous Silica Template with Water
Authors: Nador Fabiana , Guisasola Eduardo , Baeza Alejandro , Villaecija Miguel Angel Moreno , Vallet‐Regí Maria , Ruiz‐Molina Daniel 
Published: January 2017

AbstractInvited for the cover of this issue is the group of Daniel Ruiz‐Molina at the ICN2 in collaboration with the group of Maria Vallet‐Regí at UCM. The image highlights the green methodology and easy scalability of the method used. Read the full text of the article at 10.1002/chem.201604631.
Pulse‐Width Dependence of the Cooling Effect on Sub‐Micrometer ZnO Spherical Particle Formation by Pulsed‐Laser Melting in a Liquid
Authors: Sakaki Shota , Ikenoue Hiroshi , Tsuji Takeshi , Ishikawa Yoshie , Koshizaki Naoto 
Published: January 2017

AbstractSub‐micrometer spherical particles can be synthesized by irradiating particles in a liquid with a pulsed laser (pulse width: 10 ns). In this method, all of the laser energy is supposed to be spent on particle heating because nanosecond heating is far faster than particle cooling. To study the cooling effect, sub‐micrometer spherical particles are fabricated by using a pulsed laser with longer pulse widths (50 and 70 ns). From the increase in the laser‐fluence threshold for sub‐micrometer spherical particle formation with increasing pulse width, it is concluded that the particles dissipate heat to the surrounding liquid, even during several tens of nanoseconds of heating. A particle heating–cooling model considering the cooling effect is developed to estimate the particle temperature during laser irradiation. This model suggests that the liquid surrounding the particles evaporates, and the generated vapor films suppress heat dissipation from the particles, resulting in efficient heating and melting of the particles in the liquid. In the case of small particle sizes and large pulse widths, the particles dissipate heat to the liquid without forming such vapor films.
Hydrogen Oxidation‐Selective Electrocatalysis by Fine Tuning of Pt Ensemble Sites to Enhance the Durability of Automotive Fuel Cells
Authors: Yun Su‐Won , Park Shin‐Ae , Kim Tae‐June , Kim Jun‐Hyuk , Pak Gi‐Woong , Kim Yong‐Tae 
Published: January 2017

AbstractA simple, inexpensive approach is proposed for enhancing the durability of automotive proton exchange membrane fuel cells by selective promotion of the hydrogen oxidation reaction (HOR) and suppression of the oxygen reduction reaction (ORR) at the anode in startup/shutdown events. Dodecanethiol forms a self‐assembled monolayer (SAM) on the surface of Pt particles, thus decreasing the number of Pt ensemble sites. Interestingly, by controlling the dodecanethiol concentration during SAM formation, the number of ensemble sites can be precisely optimized such that it is sufficient for the HOR but insufficient for the ORR. Thus, a Pt surface with an SAM of dodecanethiol clearly effects HOR‐selective electrocatalysis. Clear HOR selectivity is demonstrated in unit cell tests with the actual membrane electrode assembly, as well as in an electrochemical three‐electrode setup with a thin‐film rotating disk electrode configuration.
Metal–Organic Coaxial Nanowire Array Electrodes Combining Large Energy Capacity and High Rate Capability
Authors: Nakanishi Hideyuki , Kikuta Ikuo , Segawa Hiroyo , Kawabata Yuto , Kishida Reiko , Norisuye Tomohisa , Tran‐Cong‐Miyata Qui 
Published: January 2017

AbstractPseudocapacitors have been widely studied in the context of their potential applications in portable electronics and energy regeneration. However, the internal resistance within these devices hampers charge transport and limits their performance. As a result, maximum charge/discharge rates are typically limited to a few hundred mV s−1 for pseudocapacitors. Beyond this limit, capacitance rapidly decreases and devices become incapable of storing energy. Here, we design electrodes in which coaxial nanowires made of highly conductive metal cores and pseudocapacitive organic shells are fabricated into a seamless, monolithic, and vertically aligned structure. The design of this structure reduces its internal resistance, and devices fabricated using these electrodes exhibit excellent energy capacity even when charged/discharged at high rates of more than a few hundred mV s−1. The energy density obtained in these devices corresponds to the maximum energy density predicted by the Trasatti method, and the coaxial‐nanowire structure of the electrodes enhances the charge storage capacity and rate capability simultaneously.
Intracerebral microdialysis coupled to LC–MS/MS for the determination tramadol and its major pharmacologically active metabolite O‐desmethyltramadol in rat brain microdialysates
Authors: Liu Mingzhou , Wang Peng , Yu Xuming , Dong Guicheng , Yue Jiang 
Published: January 2017

A rapid and sensitive method involving liquid chromatography electrospray tandem mass spectrometry (LC‐ESI‐MS/MS) coupled to an intracerebral microdialysis technique was developed for the determination and pharmacokinetic investigation of tramadol and its major active metabolite O‐desmethyltramadol (ODT) in rat brain. The microdialysis samples were separated on a C18 column and eluted with a mobile phase of acetonitrile‐water‐formic acid (50:50:0.1; v/v/v) at a flow rate of 0.3 mL/min. The ESI‐MS/MS spectra were performed in electrospray positive ion mode, and the analytes were detected by multiple reaction monitoring (MRM) of the transitions m/z [M + H]+ 264.3 → 58.2 for tramadol, m/z [M + H]+ 250.3 → 58.3 for ODT, and m/z [M + H]+ 379.4 → 264.0 for ambroxol (internal standard; IS). The total run time was 4.0 min. A lower limit of quantitation (LLOQ) was achieved as 1 ng/mL for tramadol and 0.5 ng/mL for ODT, with excellent linearity over a concentration range of 1 ~ 500 ng/mL (r > 0.99) for tramadol and 0.5 ~ 50 ng/mL for ODT (r > 0.99), respectively. The proposed method was successfully applied to the pharmacokinetic studies of tramadol and ODT in rat brain. Copyright © 2017 John Wiley & Sons, Ltd.
Integrative effects of zinc and temperature on germination in Dimorphandra wilsonii rizz.: Implications of climate changes
Authors: Bicalho Elisa Monteze , Gomes Marcelo Pedrosa , Rodrigues-Junior Ailton Gonçalves , Oliveira Túlio Gabriel Soares , Gonçalves Cintia de Almeida , Fonseca Marcia Bacelar , Garcia Queila Souza 
Published: January 2017

Abstract

The integrative effects of zinc (Zn; 0 mg L−1, 75 mg L−1, 150 mg L−1, and 200 mg L−1) and temperature (25 °C, 30 °C, and 35 °C) on seed germination of the threatened Brazilian species Dimorphandra wilsonii were evaluated. Zinc effects on seed germination were only observed at 30 °C and 35 °C. By stimulating respiration rates, rising temperatures accentuate hydrogen peroxide (H2O2) formation in germinating seeds in the presence of Zn. Seed Zn tolerance was related to the activation of enzymatic antioxidants, and ascorbate peroxidase (APX) activity had a central role in H2O2 scavenging under the highest temperatures tested. Increased APX activity allowed successful germination, whereas decreasing APX activity was accompanied by decreasing germination rates in Zn-treated seeds at 35 °C. Within a scenario of future climate change, it will be extremely important to avoid increasing Zn concentrations in natural habitats that would threaten conservation efforts directed toward this endangered plant species. Environ Toxicol Chem 2017;36:2036–2042. © 2017 SETAC
Abstract
Zinc and temperature modulate respiration activity and germination in Dimorphandra wilsonii seeds.
Head circumference, leg length and its association with dementia among older adult population in Singapore
Authors: Chang Sherilyn , Ong Hui Lin , Abdin Edimansyah , Vaingankar Janhavi Ajit , Jeyagurunathan Anitha , Shafie Saleha , Mahendran Rathi , Subramaniam Mythily , Chong Siow Ann 
Published: January 2017

BackgroundHead circumference and leg length serve as reliable proxy indicators of early‐life environment. Research studies have shown that these anthropometric measurements are associated with cognitive impairment and dementia among older adults. The aim of the present study was to assess the associations between dementia with head circumference and leg length among the older adult population in Singapore. This study also aimed to examine the sociodemographic correlates of these anthropometric measurements.MethodsData were collected from 2565 older adults aged 60 years and above, in a population study on the Well‐being of the Singapore Elderly. Head circumference and leg length measurements were obtained, and sociodemographic information was recorded. Dementia diagnosis was made using the 10/66 dementia algorithm. Anthropometric measurements were first stratified into quarters, and then logistic regression analysis was used to examine factors associated with head circumference and leg length, as well as to examine the association between dementia with these measurements.ResultsSociodemographic correlates of head circumference and leg length include age, gender, ethnicity and education level. Smaller head circumference was independently associated with higher odds of 10/66 dementia (OR = 2.173–2.709). When the regression analysis was stratified by gender, the association was found only in the male sample. Leg length was not significantly associated with dementia after controlling for sociodemographic variables.ConclusionSmaller head circumference is independently associated with dementia among older adults in Singapore. Findings from this study suggest that risk factors for dementia begin their influence in early life. Copyright © 2017 John Wiley & Sons, Ltd.
Cortical thickness and functional connectivity abnormality in chronic headache and low back pain patients
Authors: Yang Qing , Wang Zewei , Yang Lixia , Xu Yonghua , Chen Li Min 
Published: January 2017

AbstractThis study aims to characterize the psychological wellbeing of chronic headache (CH) patients, to identify cortical structural abnormalities and any associations of those abnormalities with resting state functional connectivity (rsFC), and to determine whether such rsFC abnormality is specific to CH patients. Compared with healthy controls (CONCH), CH patients suffered from mild depression, sleep disturbances, and relatively poor quality of life. CH patients also exhibited widespread cortical thickness (CT) abnormalities in left premotor (BA6), right primary somatosensory (S1) and right prefrontal (BA10) cortices, as well as in regions of default mode and executive control networks. Using cortical regions with thickness abnormality as seeds, we found cortical region pairs showed strengthened rsFC in CH patients. Using the same seeds, rsFC analysis from chronic low back pain (CLBP) patients and their controls (CONCLBP) identified abnormalities in non‐overlapping cortical region pairs. Direct comparison of rsFC between CH and CLBP patients revealed significantly differences in thirteen cortical region pairs, including the four identified in CH and CONCH comparison. Across all three groups (CH, CLBP and CON), the rsFC between left multisensory association area (BA39) and left posterior cingulate cortex (BA23) differed significantly. Eight regions showed CT abnormality in CLBP patients, two of which overlapped with those of CH patients. Our observations support the notion that CH and CLBP pain are pathological conditions, under which the brain develops distinct widespread structural and functional abnormalities. CH and CLBP groups share some similar structural abnormalities, but rsFC abnormalities in several cortical region pairs appear to be pathology‐specific. Hum Brain Mapp 38:1815–1832, 2017. © 2017 Wiley Periodicals, Inc.
An HDAC Inhibitor, Entinostat/MS-275, Partially Prevents Delayed Cranial Suture Closure in Heterozygous Runx2 Null Mice
Authors: Bae Han-Sol , Yoon Won-Joon , Cho Young-Dan , Islam Rabia , Shin Hye-Rim , Kim Bong-Soo , Lim Jin-Muk , Seo Min-Seok , Cho Seo-Ae , Choi Kang-Young , Baek Seung-Hak , Kim Hong-Gee , Woo Kyung-Mi , Baek Jeong-Hwa , Lee Yun-Sil , Ryoo Hyun-Mo 
Published: January 2017

ABSTRACT

Cleidocranial dysplasia (CCD) is an autosomal dominant skeletal disorder caused by mutations in RUNX2, coding a key transcription factor of early osteogenesis. CCD patients suffer from developmental defects in cranial bones. Despite numerous investigations and clinical approaches, no therapeutic strategy has been suggested to prevent CCD. Here, we show that fetal administration of Entinostat/MS-275, a class I histone deacetylase (HDAC)-specific inhibitor, partially prevents delayed closure of cranial sutures in Runx2+/- mice strain of C57BL/6J by two mechanisms: 1) posttranslational acetylation of Runx2 protein, which stabilized the protein and activated its transcriptional activity; and 2) epigenetic regulation of Runx2 and other bone marker genes. Moreover, we show that MS-275 stimulates osteoblast proliferation effectively both in vivo and in vitro, suggesting that delayed skeletal development in CCD is closely related to the decreased number of progenitor cells as well as the delayed osteogenic differentiation. These findings provide the potential benefits of the therapeutic strategy using MS-275 to prevent CCD. © 2017 American Society for Bone and Mineral Research.