bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SET‐Domain‐Containing Protein MdSDG26 Negatively Regulates Alternaria alternata Resistance in Apple
Authors: Shen Wenyun , Zhang Dehui , Zhang Zitong , He Jieqiang , Khalil Arij , Li Xuewei , Ma Fengwang , Guan Qingmei , Niu Chundong 
Published: September 2024

ABSTRACTApple leaf spot is one of the most devastating diseases in the apple industry, caused by Alternaria alternata f. sp mali (A. alternata). SET‐domain group (SDG) proteins function as the histone methyltransferases and participate in plant development and stress responses. However, whether SDG proteins are associated with A. alternata resistance is largely unclear. Here, we describe the pathogen‐inducible MdSDG26 gene in apple (Malus × domestica). MdSDG26 has two transcript variants that function similarly in catalyzing histone methylation and A. alternata resistance. Transient overexpression of MdSDG26 increased the global levels of H3K4me3 and H3K36me3, whereas knockdown of MdSDG26 only reduced the H3K36me3 level. Transcriptome analysis revealed that MdSDG26 affected the genome‐wide transcriptome changes in response to A. alternata infection. ChIP‐qPCR analysis demonstrated that MdSDG26 modulates the levels of H3K36me3 and H3K4me3 at both the promoter and exon regions of MdNTL9. As a negative regulator of A. alternata resistance in apples, MdNTL9 plays a pivotal role in MdSDG26‐mediated resistance to A. alternata. Therefore, our findings provide compelling evidence for the regulatory function of MdSDG26 in histone methylation and its molecular role in conferring resistance to A. alternata.
OsRHS Negatively Regulates Rice Heat Tolerance at the Flowering Stage by Interacting With the HSP Protein cHSP70‐4
Authors: Mao Xingxue , Yu Hang , Xue Jiao , Zhang Lanlan , Zhu Qingfeng , Lv Shuwei , Feng Yanzhao , Jiang Liqun , Zhang Jing , Sun Bingrui , Yu Yang , Li Chen , Ma Yamei , Liu Qing 
Published: September 2024

ABSTRACTHeat stress at the flowering stage significantly impacts rice grain yield, yet the number of identified genes associated with rice heat tolerance at this crucial stage remains limited. This study focuses on elucidating the function of the heat‐induced gene reduced heat stress tolerance 1 (OsRHS). Overexpression of OsRHS leads to reduced heat tolerance, while RNAi silencing or knockout of OsRHS enhances heat tolerance without compromising yield, as assessed by the seed setting rate. OsRHS is localized in the cytoplasm and mainly expressed in the glume and anther of spikelet. Moreover, OsRHS was found to interact with the HSP protein cHSP70‐4, and the knockout of cHSP70‐4 resulted in increased heat tolerance. Complementation assays revealed that the knockout of cHSP70‐4 could restore the compromised heat tolerance in OsRHS overexpression plants. Additional investigation reveals that elevated temperatures can amplify the bond between OsRHS and cHSP70‐4 within rice. Furthermore, our findings indicate that under heat stress conditions during the flowering stage, OsRHS plays a negative regulatory role in the expression of many stress‐related genes. These findings unveil the crucial involvement of OsRHS and cHSP70‐4 in modulating heat tolerance in rice and identify novel target genes for enhancing heat resilience during the flowering phase in rice.
Prognostic factors for wellbeing in patients with hyaline fibromatosis syndrome
Authors: Futagawa Hiroshi , Ito Shiho , Hosoi Kenji , Tamada Ikkei , Ogata Kiyokazu , Fukuda Kentaro , Yamanaka Haruka , Kuroda Maho , Suda Chiharu , Shimizu Kenji , Yoshihashi Hiroshi 
Published: September 2024

AbstractBackgroundHyaline fibromatosis syndrome (HFS) is a congenital disorder characterized by subcutaneous skin nodules, congenital multiple arthrogryposis, gingival hyperplasia, and chronic pain. The intellectual ability of patients with HFS is generally normal. This syndrome arises from variants of ANTXR2. Thus far, about 100 cases have been reported but few of these were reported from Japan.MethodsThis study reports five additional Japanese patients with genetically confirmed HFS, from unrelatd families, and discusses the clinical course and quality of life of these patients.ResultsAt our last visit the ages of the patients were 3–19 years (the median age was 5 years). All the patients had arthrogryposis, skin nodules, and gingival hyperplasia, and four patients had chronic pain, all of which are distinctive, clinical characteristics of HFS. Four of the patients (80%) had pruritic skin nodules, and three experienced sleep disruptions due to pruritis. The visceral complications are an index of HFS severity. One patient in the present cohort had a mucosal abnormality without any gastrointestinal symptoms.ConclusionPreventive and routine management of pruritis caused by skin nodules should be shared with the patient's family. Even asymptomatic patients might have endoscopic finding, which would be a soft marker that could predict the development of protein losing enteropathy.
Technological disasters in Asia: Epidemiological profile from the year 2000 to 2021
Authors: Fernández García Andrea , Gan Rick Kye , Cernuda Martínez José Antonio , Arcos González Pedro 
Published: September 2024

AbstractBackgroundTechnological disasters in Asia have significant public health and environmental implications, but there is limited epidemiological analysis of these events. This study aims to characterize the epidemiological profile of technological disasters in Asia from 2000 to 2021, focusing on morbidity and mortality trends.MethodsA retrospective descriptive observational analysis was conducted using data from emergency events database (EM‐DAT), DesInventar, NatCAt, and Sigma. The study categorized disasters into transport, industrial, and miscellaneous accidents. Statistical analyses were used to examine frequencies, trends, and correlations among the different disaster types.ResultsFrom 2000 to 2021, Asia experienced 2333 technological disasters, with transport accidents being the most frequent (55.77%), followed by industrial (26.10%) and miscellaneous accidents (18.13%). The overall trend showed a statistically significant decrease in the frequency of these disasters and in average mortality and injury rates. The study highlighted the varying impact of different disaster types, with industrial accidents causing the highest fatality and affected rates despite being less frequent than transport accidents.ConclusionsThe study indicates a declining trend in the frequency and severity of technological disasters in Asia, reflecting improved safety measures and disaster management. However, the high impact of industrial accidents underscores the need for targeted prevention strategies.
The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot
Authors: Liu Haixu , Zhang Zhuoqun , Xu Ying , Du Xiuming , Wen Jingzhi 
Published: September 2024

AbstractThe most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p < 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p < 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p < 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives.
A comparison of several separation processes for eggshell membrane powder as a natural biomaterial for skin regeneration
Authors: Esmaeili Samaneh , Rahmati Majid , Zamani Sepehr , Djalilian Ali R. , Arabpour Zohreh , Salehi Majid 
Published: September 2024

AbstractBackgroundNumerous studies have focused on skin damage, the most prevalent physical injury, aiming to improve wound healing. The exploration of biomaterials, specifically eggshell membranes (ESMs), is undertaken to accelerate the recovery of skin injuries. The membrane must be separated from the shell to make this biomaterial usable. Hence, this investigation aimed to identify more about the methods for membrane isolation and determine the most efficient one for usage as a biomaterial.Methods and materialsFor this purpose, ESM was removed from eggs using different protocols (with sodium carbonate, acetic acid, HCl, calcium carbonate, and using forceps for separation). Consequently, we have examined the membranes' mechanical and morphological qualities.ResultsAccording to the analysis of microscopic surface morphology, the membranes have appropriate porosity. MTT assay also revealed that the membranes have no cytotoxic effect on 3T3 cells. The results indicated that the ESM had acquired acceptable coagulation and was compatible with blood. Based on the obtained results, Provacol 4 (0.5‐mol HCl and neutralized with 0.1‐mol NaOH) was better than other methods of extraction and eggshell separation because it was more cell‐compatible and more compatible with blood.ConclusionThis study demonstrates that ESMs can be used as a suitable biomaterial in medical applications.
Global hotspots and trends in Acanthosis nigricans research: A bibliometric and visualized analysis
Authors: Zhang Shaobo , Yan Huixin , Sun Weichen , Li Jingnan , Xu Jing , Cao Di , Song Bailin , Wu Xingquan 
Published: September 2024

AbstractBackgroundAcanthosis nigricans (AN) is a common skin disease characterized by clear pigmented patches on the folds of the skin. However, the AN research field lacks scientific and comprehensive bibliometric analysis. This article aims to use bibliometric methods to summarize and visualize the distribution patterns, research hotspots, and development trends of AN literature.MethodsLiterature from 1900 to 2024 was retrieved from the Web of Science Core Collection database through AN's thematic search. Using CiteSpace, VOSviewer, and Excel 2019, conduct a comprehensive analysis of the number of publications, countries/regions, institutions, authors, journals, highly cited literature, keywords, and so on, and sort out the hotspots and directions of AN.ResultsFrom 1900 to 2024, 1675 publications were included in the bibliometric analysis, showing a steady annual increase in the number of publications. The United States leads in this research field, with the University of Texas System being a key research institution. The Journal of Clinical Endocrinology & Metabolism has the highest number of published papers. The most cited article is “Syndromes of Insulin Resistance and Acanthosis Nigricans: Insulin‐Receptor Disorders in Man” (1976). The author Taylor, SI, has published the most papers. “Acanthosis nigricans” is the most frequently occurring keyword. The main research hotspots and frontier areas in AN research are as follows: (1) The relationship between AN and other diseases is a popular research topic; (2) The connection between AN and insulin resistance (IR) is a current research focus; (3) Treatment of AN, with an emphasis on addressing the underlying causes and improving local skin conditions, represents the cutting edge of this field.ConclusionThis study summarizes the research trends and hotspots in the field of AN, offering valuable information and insights for scholars focused on AN scientific research, and providing a reference for future research directions.
Exploring the Convergence of Cyber–Physical Space: Multidimensional Modeling of Overtourism Interactions
Authors: Tai Kaiwing , Liao Minglei , Liu Xintao 
Published: September 2024

ABSTRACTAs technological advancements continue to blur the boundaries between cyber and physical spaces, individuals' activities are not limited to physical space and increasingly transcend singular space. Prior research on interactions between cyber and physical spaces oversimplified or even overlooked the interactions between the two spaces due to limited access to human activity big data, for instance, the implications of cyber–physical interactions for tourism. Consequently, this study proposes an overtourism index framework intended to capture travel behaviors in both cyber and physical spaces to address the research gap. Based on extensive social media data from Hong Kong, the proposed framework is tested and validated efficiently to emulate tourism interactions between cyber and physical spaces at fine spatiotemporal resolutions. The results indicate that there is strong convergent interaction among the public in both cyber and physical spaces. Moreover, an online WebGIS interactive platform (https://arcg.is/0mzHyH) has been developed for visualizing these interactions and provides better decision‐making regarding tourism policy in Hong Kong.
The prevalence of hepatitis B virus, human T‐lymphotropic virus and human immunodeficiency virus in patients receiving blood transfusions in South Africa
Authors: Willemse Reynier J. , Grobler Christa J. , Murphy Edward L. , Roubinian Nareg , Colemen Charl , Machaba Solly , Vermeulen Marion 
Published: September 2024

AbstractBackground and ObjectivesSouth Africa has a high prevalence of human immunodeficiency virus (HIV) and hepatitis B virus (HBV) and to a lesser extent human T‐lymphotropic virus (HTLV). Each of these agents is transfusion‐transmissible (TT) but deciding whether to implement preventive screening depends upon knowledge of background prevalence in transfused patients. We determined the prevalence of HIV, HBV and HTLV I/II among blood transfusion recipients in South African hospitals.Materials and MethodsWe obtained identity‐unlinked samples used for blood cross‐matching at 634 South African hospitals served by the South African National Blood Service (SANBS). The ABBOTT Alinity S® Immunochemiluminescent system measured HIV, HBV and HTLV I/II antibodies. Repeatedly reactive samples were confirmed using the Roche Cobas® 8000. Logistic regression was performed to investigate the determinants of associations for HIV, HBV and HTLV infections.ResultsThe overall prevalences of HIV, HBV and HTLV were 37.8%, 7.4% and 0.6%, respectively. The HIV prevalence in blood recipients was twice as high as general population estimates. Public hospital patients had a significantly higher prevalence compared with private hospital patients for HIV and HBV. HIV prevalence was significantly higher in females, and HBV prevalence was significantly higher in males, excluding the unknown gender results.ConclusionPatients receiving blood transfusions in South Africa have high rates of HIV and HBV infection that should be taken into consideration when determining donor screening strategies for other viral infections. Measurable prevalence of HTLV indicates endemicity of this infection in South Africa.
Nonlinear Dynamics of a Magnetic Shape Memory Alloy Oscillator
Authors: Souza Jean M. , Monteiro Luciana Loureiro S. , Savi Marcelo A. 
Published: September 2024

Abstract
Magnetic shape memory alloys (MSMAs) constitute a class of smart materials capable of exhibiting large magnetic field induced strain (MFIS) when subjected to magnetomechanical loadings. Two distinct mechanisms are responsible for the induced strain: martensitic variant reorientation and phase transformation. The martensitic reorientation is the most explored mechanism presenting the advantage to potential provide high-frequency actuation since it does not rely on phase transformation cycles. Despite its capabilities and potential dynamical applications, the dynamical behavior of MSMAs is not extensively explored in the literature that is usually focused on quasi-static behavior. Thereby, the objective of this work is to analyze the nonlinear dynamics of MSMAs. In this regard, an MSMA nonlinear oscillator is investigated, exploiting the system response under different bias magnetic field levels and actuation frequencies. A phenomenological model is employed to describe the MSMA magnetomechanical behavior. Numerical simulations are carried out using the operator split technique together with an iterative process and the fourth-order Runge–Kutta method. Results show that the application of a bias magnetic field can reduce the mean displacement of the system, increasing the oscillation amplitude. Furthermore, the period of oscillation can be modified, even achieving complex behaviors, including chaos. The potential use of MSMAs to dynamical systems is explored showing the possibility to provide adaptive behaviors.
Context-Driven Design of a Laparoscopic Instrument Cleaner for Use in Rural Low-Resource Hospitals
Authors: Robertson Daniel , Kok Abe , Oosting Roos , Gnanaraj Jesudian , Buzink Sonja , Dankelman Jenny 
Published: September 2024

Abstract
Laparoscopic surgery offers significant benefits to patients in low-resource settings compared to open surgery such as faster recovery, less pain, and lower infection rate. However, there exist significant barriers to the safe introduction of laparoscopy such as high costs and limited availability of trained staff. Low- and middle-income country (LMIC) hospitals suffer from higher post-surgical infection which might be due to the limited facilities for the sterile reprocessing of laparoscopic instruments. To design a solution to this issue, a detailed understanding of local settings was needed. Therefore, this research applied a context-driven design approach, based on the Roadmap for Design of Surgical Equipment for Safe Surgery Worldwide. Over several design phases, the need for a reprocessing device was established. An analysis of the sterile reprocessing of laparoscopic instruments led to a list of context-specific design requirements. These were translated to a final conceptual design of a laparoscopic instrument cleaner using a waterfall design method. Finally, a usability study of the loading system of the device was conducted with nurses in four Indian hospitals. A root-cause analysis of the usability study showed that the device was not intuitive enough to use for Indian nurses. A redesign of the loading system was made to improve its ease of use. The design process used in this study can be used as an example for designers wanting to address the critical issue of context-specific medical devices worldwide, or more specifically, the sterile supply of surgical instruments in resource-constrained environments.
Crack Growth Prediction Based on Uncertain Parameters Using Ensemble Kalman Filter
Authors: Hana Norihiko , Hatanaka Rinya , Amaya Kenji 
Published: September 2024

Abstract
We are developing a method that will enable the estimation of crack shapes in such structures as power equipment and social infrastructure with greater precision as well as the prediction of crack growth life under conditions of uncertainty regarding crack perimeter structure and applied loads. Ascertaining the dimensions of cracks is complicated by the influences exerted by external loads on crack propagation as well as the geometry of crack perimeters. The prediction of crack propagation based on uncertain information is an overly conservative approach due to the lack of accuracy. This paper presents a Bayesian estimation of actual crack geometry based on predictions from a physical model of crack growth and measured crack geometry. The uncertainty in the load and the geometry of the crack perimeter are reflected in the crack propagation model. The range over which the uncertain parameters are estimated is updated simultaneously with estimations of the crack shape. Furthermore, we describe how optimal measurement intervals can be identified from the one-period-ahead prediction of crack growth based on a physical model. The application of properly spaced measurements and sequential Bayesian estimation can effectively mitigate the impact of measurement error and parameter uncertainty, thereby enhancing the precision of crack growth prediction. Sequential Bayesian estimation is an Ensemble Kalman Filter, and our physical model of crack propagation is a Paris measure based on fracture mechanics. The efficacy of the methodology presented in this paper is validated by the outcomes of the simulated observed data of a CT specimen.
Impact of Encapsulated Phase Change Material Additives for Improved Thermal Performance of Silicone Gel Insulation
Authors: Kasitz Joshua , Ghufran Muhammad , Huitink David 
Published: September 2024

Abstract
Passive cooling through phase change materials (PCM) creates beneficial complimentary cooling techniques aimed at providing thermal gradient mitigation during device operation without additional power requirements. These have been well studied but are difficult to implement due to complications concerning effective enclosure of the liquid phase. Encapsulated PCM particles can be embedded in other materials to form composites with form stable solid–liquid phase transitions. This study characterizes a new composite of silicone gel and encapsulated phase change materials (ePCMs) for use as an encapsulant. The ePCMs contain a paraffin core and titania shell resulting in a self-contained solid–liquid phase transition producing an average of 132.9 J/g of latent heat capacity. The gel composites gain latent heat capacity as a linear function of ePCM concentration by weight. The 30% ePCM sample contains 41.0 J/g of latent heat capacity, approximately 30% of ePCM control samples. The specific heat capacity of the silicone gel without ePCMs is 1.539 J/g-° C and 2.825 J/g-° C for the ePCM particles. As the ePCM concentration increases, the specific heat capacity is increased toward the highest value of the pure ePCMs across all temperature ranges. The coefficient of thermal expansion of the composites is increased with ePCM concentration up to a maximum of 96% in the 20% ePCM concentration. The elastic modulus remains relatively constant across ePCM concentrations and temperatures. In the needle–needle breakdown voltage testing the 20% sample has a 6 kV/mm reduction in dielectric strength and higher than 20% ePCM samples show increased variability in strength due to the dispersed particles. Overall, the results from these material characterizations demonstrate the promise of dielectric composites containing ePCM particles to add passive cooling capability into electronics devices without complex structures.