bluuebunny/crossref_metadata_2025_split huggingface dataset:
HMGCS2 is a key ketogenic enzyme potentially involved in type 1 diabetes with high cardiovascular risk
Authors: Shukla Sanket Kumar , Liu Weijing , Sikder Kunal , Addya Sankar , Sarkar Amrita , Wei Yidong , Rafiq Khadija 
Published: June 2017

AbstractDiabetes increases the risk of Cardio-vascular disease (CVD). CVD is more prevalent in type 2 diabetes (T2D) than type 1 diabetes (T1D), but the mortality risk is higher in T1D than in T2D. The pathophysiology of CVD in T1D is poorly defined. To learn more about biological pathways that are potentially involved in T1D with cardiac dysfunction, we sought to identify differentially expressed genes in the T1D heart. Our study used T1D mice with severe hyperglycemia along with significant deficits in echocardiographic measurements. Microarray analysis of heart tissue RNA revealed that the T1D mice differentially expressed 10 genes compared to control. Using Ingenuity Pathway Analysis (IPA), we showed that these genes were significantly involved in ketogenesis, cardiovascular disease, apoptosis and other toxicology functions. Of these 10 genes, the 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (HMGCS2) was the highest upregulated gene in T1D heart. IPA analysis showed that HMGCS2 was center to many biological networks and pathways. Our data also suggested that apart from heart, the expression of HMGCS2 was also different in kidney and spleen between control and STZ treated mice. In conclusion, The HMGCS2 molecule may potentially be involved in T1D induced cardiac dysfunction.
Clinicopathological features and prognosis of gastric adenosquamous carcinoma
Authors: Feng Fan , Zheng Gaozan , Qi Jingpeng , Xu Guanghui , Wang Fei , Wang Qiao , Guo Man , Lian Xiao , Zhang Hongwei 
Published: June 2017

AbstractData about primary gastric adenosquamous carcinoma (ASC) was limited due to rare incidence. Thus, the present study aims to investigate clinicopathological features and prognosis of gastric ASC. Cases of gastric ASC were obtained from our center and from case reports and series extracted from Medline. Clinicopathological features and prognosis of gastric ASC were analyzed and compared with gastric adenocarcinoma (AC) in our center. The commonest location was lower third (45.0%), followed by upper (26.2%) and middle third (24.2%). The median tumor size was 6 cm (0.8–17). The commonest differentiation status was well for both AC (44.4%) and SCC components (40.9%). Half of tumors (52.7%) were stage T4 and most patients (86.2%) suffered from lymph node metastasis (LNM). Tumor depth and TNM stage were risk factors for overall survival (OS) (both P < 0.05). The distribution of age, tumor size, tumor location, tumor depth, LNM and TNM stage were significantly different between gastric ASC and AC (all P < 0.05). The OS of gastric ASC was significantly worse than AC (P < 0.001), especially in stage III disease (P < 0.001). Gastric ASC differ significantly from AC with respect to clinicopathological features. The prognosis of gastric ASC was worse than AC.
Formulation and candidacidal activity of magnetic nanoparticles coated with cathelicidin LL-37 and ceragenin CSA-13
Authors: Niemirowicz Katarzyna , Durnaś Bonita , Tokajuk Grażyna , Piktel Ewelina , Michalak Grzegorz , Gu Xiaobo , Kułakowska Alina , Savage Paul B. , Bucki Robert 
Published: June 2017

AbstractFungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C. albicans, C. glabrata and C. tropicalis were evaluated. These experiments confirm the high anti-fungal activity of these well-characterized agents mediated by their interaction with the fungal membrane and demonstrate elevated activity following immobilization of LL-37 and CSA-13 on the surface of magnetic nanoparticles (MNPs). Furthermore, MNP-based nanosystems are resistant to inhibitory factors present in body fluids and effectively inhibit formation of fungal biofilm. Simultaneously, synthesized nanostructures maintain immunomodulatory properties, described previously for free LL-37 peptide and CSA-13 substrate and they do not interfere with the proliferation and viability of osteoblasts, confirming their high biocompatibility.
CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus
Authors: Heinze Svenia D. , Kohlbrenner Tea , Ippolito Domenica , Meccariello Angela , Burger Alexa , Mosimann Christian , Saccone Giuseppe , Bopp Daniel 
Published: June 2017

AbstractThe classic brown body (bwb) mutation in the housefly Musca domestica impairs normal melanization of the adult cuticle. In Drosophila melanogaster, a reminiscent pigmentation defect results from mutations in the yellow gene encoding dopachrome conversion enzyme (DCE). Here, we demonstrate that the bwb locus structurally and functionally represents the yellow ortholog of Musca domestica, MdY. In bwb Musca strains, we identified two mutant MdY alleles that contain lesions predicted to result in premature truncation of the MdY open reading frame. We targeted wildtype MdY by CRISPR-Cas9 RNPs and generated new mutant alleles that fail to complement existing MdY alleles, genetically confirming that MdY is the bwb locus. We further found evidence for Cas9-mediated interchromosomal recombination between wildtype and mutant bwb alleles. Our work resolves the molecular identity of the classic bwb mutation in Musca domestica and establishes the feasibility of Cas9-mediated genome editing in the Musca model.
Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
Authors: Jiang Tao , Munguia-Lopez Jose G. , Flores-Torres Salvador , Grant Joel , Vijayakumar Sanahan , Leon-Rodriguez Antonio De , Kinsella Joseph M. 
Published: June 2017

AbstractHuman tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After ~15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue co-culture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks.
Diffraction phase microscopy imaging and multi-physics modeling of the nanoscale thermal expansion of a suspended resistor
Authors: Wang Xiaozhen , Lu Tianjian , Yu Xin , Jin Jian-Ming , Goddard Lynford L. 
Published: June 2017

AbstractWe studied the nanoscale thermal expansion of a suspended resistor both theoretically and experimentally and obtained consistent results. In the theoretical analysis, we used a three-dimensional coupled electrical-thermal-mechanical simulation and obtained the temperature and displacement field of the suspended resistor under a direct current (DC) input voltage. In the experiment, we recorded a sequence of images of the axial thermal expansion of the central bridge region of the suspended resistor at a rate of 1.8 frames/s by using epi-illumination diffraction phase microscopy (epi-DPM). This method accurately measured nanometer level relative height changes of the resistor in a temporally and spatially resolved manner. Upon application of a 2 V step in voltage, the resistor exhibited a steady-state increase in resistance of 1.14 Ω and in relative height of 3.5 nm, which agreed reasonably well with the predicted values of 1.08 Ω and 4.4 nm, respectively.
Identifying Human SIRT1 Substrates by Integrating Heterogeneous Information from Various Sources
Authors: Zhai Zichao , Tang Ming , Yang Yue , Lu Ming , Zhu Wei-Guo , Li Tingting 
Published: June 2017

AbstractMost proteins undergo different kinds of modification after translation. Protein acetylation is one of the most crucial post-translational modifications, which causes direct or indirect impact on various biological activities in vivo. As a member of Class III HDACs, SIRT1 was the closest one to the yeast sir2 and drew most attention, while a small number of known SIRT1 substrates caused difficulties to clarify its function. In this work, we designed a novel computational method to screen SIRT1 substrates based on manually collected data and Support Vector Machines (SVMs). Unlike other approaches, we took both primary sequence and protein functional features into consideration. Through integrating functional features, the Matthews correlation coefficient (MCC) for the prediction increased from 0.10 to 0.65. The prediction results were verified by independent dataset and biological experiments. The validation results demostrated that our classifier could effectively identify SIRT1 substrates and filter appropriate candidates for further research. Furthermore, we provide online tool to support SIRT1 substrates prediction, which is freely available at http://bioinfo.bjmu.edu.cn/huac/.
Maternal HtrA3 optimizes placental development to influence offspring birth weight and subsequent white fat gain in adulthood
Authors: Li Ying , Salamonsen Lois A. , Hyett Jonathan , Costa Fabricio da Silva , Nie Guiying 
Published: June 2017

AbstractHigh temperature requirement factor A3 (HtrA3), a member of the HtrA protease family, is highly expressed in the developing placenta, including the maternal decidual cells in both mice and humans. In this study we deleted the HtrA3 gene in the mouse and crossed females carrying zero, one, or two HtrA3-expressing alleles with HtrA3+/− males to investigate the role of maternal vs fetal HtrA3 in placentation. Although HtrA3−/− mice were phenotypically normal and fertile, HtrA3 deletion in the mother resulted in intra-uterine growth restriction (IUGR). Disorganization of labyrinthine fetal capillaries was the major placental defect when HtrA3 was absent. The IUGR caused by maternal HtrA3 deletion, albeit being mild, significantly altered offspring growth trajectory long after birth. By 8 months of age, mice born to HtrA3-deficient mothers, independent of their own genotype, were significantly heavier and contained a larger mass of white fat. We further demonstrated that in women serum levels of HtrA3 during early pregnancy were significantly lower in IUGR pregnancies, establishing an association between lower HtrA3 levels and placental insufficiency in the human. This study thus revealed the importance of maternal HtrA3 in optimizing placental development and its long-term impact on the offspring well beyond in utero growth.
Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
Authors: Lázaro-Díez María , Chapartegui-González Itziar , Redondo-Salvo Santiago , Leigh Chike , Merino David , Segundo David San , Fernández Adrián , Navas Jesús , Icardo José Manuel , Acosta Félix , Ocampo-Sosa Alain , Martínez-Martínez Luis , Ramos-Vivas José 
Published: June 2017

AbstractAcinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
Near-field fault slip of the 2016 Vettore Mw 6.6 earthquake (Central Italy) measured using low-cost GNSS
Authors: Wilkinson Maxwell W. , McCaffrey Ken J. W. , Jones Richard R. , Roberts Gerald P. , Holdsworth Robert E. , Gregory Laura C. , Walters Richard J. , Wedmore Luke , Goodall Huw , Iezzi Francesco 
Published: June 2017

AbstractThe temporal evolution of slip on surface ruptures during an earthquake is important for assessing fault displacement, defining seismic hazard and for predicting ground motion. However, measurements of near-field surface displacement at high temporal resolution are elusive. We present a novel record of near-field co-seismic displacement, measured with 1-second temporal resolution during the 30th October 2016 Mw 6.6 Vettore earthquake (Central Italy), using low-cost Global Navigation Satellite System (GNSS) receivers located in the footwall and hangingwall of the Mt. Vettore - Mt. Bove fault system, close to new surface ruptures. We observe a clear temporal and spatial link between our near-field record and InSAR, far-field GPS data, regional measurements from the Italian Strong Motion and National Seismic networks, and field measurements of surface ruptures. Comparison of these datasets illustrates that the observed surface ruptures are the propagation of slip from depth on a surface rupturing (i.e. capable) fault array, as a direct and immediate response to the 30th October earthquake. Large near-field displacement ceased within 6–8 seconds of the origin time, implying that shaking induced gravitational processes were not the primary driving mechanism. We demonstrate that low-cost GNSS is an accurate monitoring tool when installed as custom-made, short-baseline networks.
Visible-light driven Photoelectrochemical Immunosensor Based on SnS2@mpg-C3N4 for Detection of Prostate Specific Antigen
Authors: Zhang Yifeng , Liu Yixin , Li Rongxia , Saddam Khan Malik , Gao Picheng , Zhang Yong , Wei Qin 
Published: June 2017

AbstractHerein, a novel label-free photoelectrochemical (PEC) immunosensor based on SnS2@mpg-C3N4 nanocomposite is fabricated for the detection of prostate specific antigen (PSA) in human serum. Firstly, mesoporous graphite-like carbon nitride (mpg-C3N4) with carboxyl groups is synthesized successfully which possesses high specific surface area and large pore volume. Then, SnS2 as a typical n-type semiconductor with weak photoelectric conversion capability is successfully loaded on carboxylated mpg-C3N4 to form a well-matched overlapping band-structure. The as-synthesized SnS2@mpg-C3N4 nanocomposite performs outstanding photocurrent response under visible-light irradiation due to low recombination rate of photoexcited electron-hole pairs, which is transcend than pure SnS2 or pure mpg-C3N4. It is worth noting that SnS2@mpg-C3N4 nanocomposite is firstly employed as the photoactive material in PEC immunosensor area. The concentration of PSA can be analyzed by the decrease in photocurrent resulted from increased steric hindrance of the immunocomplex. Under the optimal conditions, the developed PEC immunosensor displays a liner photocurrent response in the range of 50 fg·mL−1 ~ 10 ng·mL−1 with a low detection limit of 21 fg·mL−1. Furthermore, the fabricated immunosensor with satisfactory stability, reproducibility and selectivity provides a novel method for PSA determination in real sample analysis.
Polysyllable Speech Accuracy and Predictors of Later Literacy Development in Preschool Children With Speech Sound Disorders
Authors: Masso Sarah , Baker Elise , McLeod Sharynne , Wang Cen 
Published: June 2017

Purpose
The aim of this study was to determine if polysyllable accuracy in preschoolers with speech sound disorders (SSD) was related to known predictors of later literacy development: phonological processing, receptive vocabulary, and print knowledge. Polysyllables—words of three or more syllables—are important to consider because unlike monosyllables, polysyllables have been associated with phonological processing and literacy difficulties in school-aged children. They therefore have the potential to help identify preschoolers most at risk of future literacy difficulties.

Method
Participants were 93 preschool children with SSD from the Sound Start Study. Participants completed the Polysyllable Preschool Test (Baker, 2013) as well as phonological processing, receptive vocabulary, and print knowledge tasks.

Results
Cluster analysis was completed, and 2 clusters were identified: low polysyllable accuracy and moderate polysyllable accuracy. The clusters were significantly different based on 2 measures of phonological awareness and measures of receptive vocabulary, rapid naming, and digit span. The clusters were not significantly different on sound matching accuracy or letter, sound, or print concept knowledge.

Conclusions
The participants' poor performance on print knowledge tasks suggested that as a group, they were at risk of literacy difficulties but that there was a cluster of participants at greater risk—those with both low polysyllable accuracy and poor phonological processing.
Facile synthesis of reduced graphene oxide/NH4V3O8 with high capacity as a cathode material for lithium ion batteries
Authors: Kou Lingjiang , Cao Liyun , Huang Jianfeng , Yang Jun 
Published: June 2017

Owing to low‐electronic conductivity and long lithium ion diffusion path of NH4V3O8 limit its application for lithium‐ion batteries (LIBs). To overcome these limitation, a new nano‐composites material of NH4V3O8‐based have been successfully fabricated by a facial and environmental friendly approach, without the addition of any other template or surfactant. A facile hydrothermal route is employed to synthesised the reduced graphene oxide(rGO)/NH4V3O8 composites. Results show that the NH4V3O8 nano‐belt are well distributed on the surface of rGO nanosheets. The resulted rGO/NH4V3O8 nanocomposites exhibit a high capacity as a cathode material for LIBs in comparison with the bare NH4V3O8. When used as cathode material for LIBs, it delivers a maximum discharge capacity of 253 mAhg−1 at 15 mAg−1, 70 mAhg−1 larger than that of the pristine one. The enhanced electrochemical performance is attributed to the synergetic effects between NH4V3O8 and rGO, such as increased conductivity and shortened lithium ion diffusion path.
Fertility Status and Cancer
Authors: Brinton Louise 
Published: June 2017

AbstractElevated estradiol levels associated with ovulation-stimulating drugs used to treat infertility and ovarian punctures involved with in vitro fertilization (IVF) have led to concerns regarding long-term cancer effects. Assessed risks, however, have been contradictory. This may reflect small sample sizes and short follow-up times, as well as an inability of many studies to adjust for appropriate potential confounders, such as causes of infertility and parity. While early studies of ovulation-stimulating drugs suggested large increases in large ovarian cancer risk, more recent studies have not confirmed this. However, several investigations have noted risk elevations associated with drug use or IVF among women who remain nulliparous, possibly due to resistant infertility. Studies also suggest increases in borderline ovarian tumors, although the effects of surveillance bias are unclear. Investigations have not shown large increases in breast cancer risk, although a few studies suggest possible increases in select subgroups, including nulliparous women or those with high drug dosages in the distant past. A few studies have evaluated risks of other cancers (endometrium, colorectum, thyroid, and melanoma), without definitive results. Since women exposed to fertility treatments are just beginning to enter typical cancer age ranges, further well-designed investigations are needed to fully delineate long-term effects.
Male Infertility and Risk of Nonmalignant Chronic Diseases: A Systematic Review of the Epidemiological Evidence
Authors: Bonde Jens , Eisenberg Michael , Giwercman Aleksander , Hærvig Katia , Rimborg Susie , Vassard Ditte , Pinborg Anja , Schmidt Lone , Bräuner Elvira , Glazer Clara 
Published: June 2017

AbstractThe association between male infertility and increased risk of certain cancers is well studied. Less is known about the long-term risk of nonmalignant diseases in men with decreased fertility. A systemic literature review was performed on the epidemiologic evidence of male infertility as a precursor for increased risk of diabetes, cardiovascular diseases, and all-cause mortality. PubMed and Embase were searched from January 1, 1980, to September 1, 2016, to identify epidemiological studies reporting associations between male infertility and the outcomes of interest. Animal studies, case reports, reviews, studies not providing an accurate reference group, and studies including infertility due to vasectomy or malignancy were excluded. The literature search resulted in 2,485 references among which we identified seven articles fulfilling the eligibility criteria. Of these, four articles were prospective (three on risk of mortality, one on risk of chronic diseases) and three were cross-sectional relating male infertility to the Charlson Comorbidity Index. The current epidemiological evidence is compatible with an association between male infertility and risk of chronic disease and mortality, but the small number of prospective studies and insufficient adjustment of confounders preclude strong statements about male infertility as precursor of these outcomes.
Environmental Contaminants Affecting Fertility and Somatic Health
Authors: Chiang Catheryne , Mahalingam Sharada , Flaws Jodi 
Published: June 2017

AbstractThis review article summarizes the epidemiological findings published between 2011 and 2016 concerning bisphenol A (BPA), phthalates, dioxins, pesticides, air pollution, fracking chemicals, triclosan, and parabens and fertility parameters in men (i.e., semen volume, sperm concentration, sperm motility, and sperm morphology) as well as fertility parameters in women (i.e., cyclicity, fertility treatment outcomes), pregnancy outcomes (i.e., preterm birth, miscarriage), and reproductive disorders (i.e., polycystic ovary syndrome, endometriosis, and uterine fibroids). Overall, this review indicates that several environmental toxicants are significantly associated with reduced fertility parameters in men and women as well as several reproductive disorders in women. Although many studies reported that the selected exposures are associated with adverse fertility outcomes, several studies reported null associations. Thus, future studies are still needed to better elucidate the associations and potential mechanisms between these environmental chemicals and fertility outcomes in men and women.