bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monocytes incubated with surfactant: a model for human alveolar macrophages?
Authors: Geertsma Minke F , Van Furth Ralph , Nibbering Peter H 
Published: January 2018

Abstract
Monocytes migrate to the lungs and enter the alveoli where they come into contact with surfactant and differentiate into alveolar macrophages. This study focused on the question of the extent to which monocytes and monocyte-derived macrophages (MDM) incubated with surfactant resemble alveolar macrophages. Surfactant-incubated monocytes shared with alveolar macrophages the intracellular presence of surfactant, efficient phagocytosis of opsonized Staphylococcus aureus, and poor intracellular killing of ingested bacteria. The suppressive effect of surfactant on bactericidal activities of monocytes could not be attributed to either the surfactant lipid fraction or surfactant protein A. Monocytes incubated with surfactant differed from alveolar macrophages with respect to expression of various Fc and complement receptors involved in intracellular killing of bacteria. Surfactant-incubated monocytes produced significantly more H2O2 upon stimulation with phorbol ester than alveolar macrophages, but significantly less than control monocytes. Together, monocytes and MDM incubated with surfactant, although similar to alveolar macrophages in some aspects, are not an adequate model for alveolar macrophages. Most likely, factors other than surfactant in the microenvironment of the alveoli, such as oxygen tension, play a role in the differentiation of monocytes to alveolar macrophages as well.
Granulocyte chemotactic protein-2 and related CXC chemokines: from gene regulation to receptor usage
Authors: Van Damme Jo , Wuyts Anja , Froyen Guy , Van Coillie Els , Struyf Sofie , Billiau Alfons , Proost Paul , Wang Ji Ming , Opdenakker Ghislain 
Published: January 2018

Abstract
Chemokines contribute to the inflammatory response by selective attraction of various leukocytic cell types. Human GCP-2 was originally identified by amino acid sequence analysis as a CXC chemokine co-produced with IL-8 by osteosarcoma cells. Furthermore, the complete coding domain of human GCP-2 was disclosed by means of RT-PCR. Similarly, mouse GCP-2 was isolated from fibroblastoid and epithelial cells and completely identified by sequence analysis. Human and mouse GCP-2 share 61% identical amino acids. Both chemokines occur as multiple NH2-terminally truncated forms. The shorter forms of mouse, but not those of human, GCP-2 showed a higher neutrophil chemotactic potency and gelatinase B releasing capacity. Mouse GCP-2 was a more potent neutrophil activator than human GCP-2, natural mouse KC, and MIP-2. Human GCP-2 was not chemotactic for monocytes, lymphocytes, or eosinophils. Quantitative studies of mRNA expression in diploid fibroblasts revealed GCP-2 induction by IL-1β. Human GCP-2 induced [Ca2+]i increase in neutrophils, which was reciprocally desensitized by IL-8, GROα, and ENA-78. Human GCP-2 induced [Ca2+]i increases and chemotactic responses in both CXCR1- and CXCR2-transfected cells. Finally, GCP-2 provoked neutrophil accumulation and plasma extravasation in rabbit skin. In humans, GCP-2 complements the activity of IL-8 as neutrophil chemoattractant and activator but it constitutes a major neutrophil chemokine in the mouse. GCP-2 induces neutrophil chemotaxis and activation but it might also contribute to detrimental tissue damage in sepsis, acute respiratory distress syndrome, acute hypersensitivity reactions, and autoimmune diseases. It might also influence the invasive capacity of GCP-2-secreting tumor cells.
Pivotal role of interleukin-8 in the acute respiratory distress syndrome and cerebral reperfusion injury
Authors: Matsumoto Tetsuya , Yokoi Kenji , Mukaida Naofumi , Harada Akihisa , Yamashita Junkoh , Watanabe Yoh , Matsushima Kouji 
Published: January 2018

Abstract
Neutrophil recruitment is one of the hallmarks of acute inflammation. A potent neutrophil chemotactic and activating factor, interleukin-8 (IL-8), has been demonstrated to be elevated in body fluids in various human diseases and experimental animal models. Recent investigations on animal disease models using blocking antibodies to IL-8 have revealed the essential involvement of IL-8 in acute inflammation. We previously reported that the administration of a neutralizing antibody against IL-8 prevented the neutrophil infiltration and neutrophil-mediated tissue injury in several animal studies. In addition, we have recently demonstrated that anti-IL-8 treatment is also effective in prevention of two models that are very relevant to clinical situations: cerebral reperfusion injury and endotoxemia-induced acute respiratory distress syndrome-like lung injury. These results further support the hypothesis that IL-8 has a pivotal role and is a novel target for therapeutic intervention in neutrophil-mediated injury.
Sequence similarities of a subgroup of CXC chemokines related to murine LIX: implications for the interpretation of evolutionary relationships among chemokines
Authors: Smith Jeffrey B , Rovai Leonor E , Herschman Harvey R 
Published: January 2018

Abstract
The murine CXC chemokine LIX has distinctive sequence features that suggest it is a novel chemokine. Among known human chemokines, ENA-78 and GCP-2 are the two most closely related to LIX. We have recently cloned the human GCP-2 gene. Phylogenetic analysis shows that the LIX coding region is more distant from both human GCP-2 and ENA-78 than is porcine AMCF-II, the chemokine with greatest sequence similarity to LIX. Human GCP-2 and ENA-78 have very high nucleotide similarity in non-coding as well as coding sequences, which suggests that these genes are the result of an evolutionarily recent gene duplication event. If this duplication occurred during primate evolution, then non-primate species may have only a single chemokine corresponding to this pair of human genes. This example shows that a one-to-one genetic correspondence does not necessarily exist between all the chemokine genes in two different species. These observations may have important implications for other chemokines that belong to clusters of closely related genes.
Effect of mast cell deficiency and leukotriene inhibition on the influx of eosinophils induced by eotaxin
Authors: Harris R R , Komater V A , Marett R A , Wilcox D M , Bell R L 
Published: January 2018

Abstract
Eosinophils are believed to be important cells in the pathogenesis of asthma and allergic disease. Mast cells and leukotrienes may play a role in eosinophil recruitment. Eotaxin was recently described as a specific chemoattractant for eosinophils. Therefore we examined the effects of eotaxin on eosinophil flux in the mast cell-deficient WBB6F1/J-Kitw/ Kitw-v (W/Wv) mice and in mice treated with zileuton or ABT-761, specific 5-lipoxygenase inhibitors. Mice were injected intraperitoneally with eotaxin and at various times later the peritoneal cavities were lavaged and cell populations determined. Murine recombinant eotaxin induced a dose-dependent increase in eosinophils, which reached a maximum at 1-2 h and subsided at 4 h in both BALB/c and W/Wv littermate control mice (no other cell population was altered). However, in eotaxin-injected W/Wv mice, the peak of eosinophil influx was delayed, peaking at 2 h, and a lower number of eosinophils was seen. The specific lipoxygenase inhibitors zileuton and ABT-761 given 30 min before eotaxin caused a 63-79% reduction in the level of eosinophils seen in the lavage fluid. These data suggest that eotaxin may either be activating eosinophils to release leukotrienes or making them more responsive to leukotrienes. In addition, mast cells may be playing a role in the amplification of the eotaxin effect.
Estimating uncertainty associated with the standardized precipitation index
Authors: Carbone Gregory J. , Lu Junyu , Brunetti Michele 
Published: January 2018

ABSTRACTWe investigate methodological uncertainties associated with the standardized precipitation index (SPI) that result from limited record length, trends, and outliers. We use long, homogenous records from 14 Italian stations to investigate how specific features in the precipitation record affect construction of an underlying gamma probability function. We apply a resampling scheme to the long records in order to estimate confidence intervals associated with a range of precipitation characteristics. Stability in parameter estimation increases nonlinearly as record length increases. The resulting SPI estimates for 30‐year reference periods have considerably more uncertainty than those made from 60‐year records. In general, increasing record length beyond 60‐years has limited benefits and, in the presence of a trend, may increase uncertainty. Extreme events also have significant influence on SPI estimates, even for records exceeding 60 years. Despite using stations from different geographic regions, each with unique precipitation characteristics, we find consistent confidence interval estimates across stations. These confidence intervals can be applied to specific time series to identify how trends, changes in variability, and outliers during a particular reference period influence SPI values.
Current developments in homonuclear and heteronuclear J‐resolved NMR experiments
Authors: Parella Teodor 
Published: January 2018

Two‐dimensional J‐resolved (Jres) NMR experiments offer a simple, user‐friendly spectral representation where the information of coupling constants and chemical shifts are separated into two orthogonal frequency axis. Since its initial proposal 40 years ago, Jres has been the focus of considerable interest both in improving the basic pulse sequence and in its successful application to a wide range of studies. Here, the latest developments in the design of novel Jres pulse schemes are reviewed, mainly focusing on obtaining pure absorption lineshapes, minimizing strong coupling artifacts, and also optimizing sensitivity and experimental measurements. A discussion of several Jres versions for the accurate measurement of a different number of homonuclear (JHH) and heteronuclear (JCH) coupling constants is presented, accompanied by some illustrative examples.
Nonrigid active shape model–based registration framework for motion correction of cardiac T1 mapping
Authors: El‐Rewaidy Hossam , Nezafat Maryam , Jang Jihye , Nakamori Shiro , Fahmy Ahmed S. , Nezafat Reza 
Published: January 2018

PurposeAccurate reconstruction of myocardial T1 maps from a series of T1‐weighted images consists of cardiac motions induced from breathing and diaphragmatic drifts. We propose and evaluate a new framework based on active shape models to correct for motion in myocardial T1 maps.MethodsMultiple appearance models were built at different inversion time intervals to model the blood‐myocardium contrast and brightness changes during the longitudinal relaxation. Myocardial inner and outer borders were automatically segmented using the built models, and the extracted contours were used to register the T1‐weighted images. Data acquired from 210 patients using a free‐breathing acquisition protocol were used to train and evaluate the proposed framework. Two independent readers evaluated the quality of the T1 maps before and after correction using a four‐point score. The mean absolute distance and Dice index were used to validate the registration process.ResultsThe testing data set from 180 patients at 5 short axial slices showed a significant decrease of mean absolute distance (from 3.3 ± 1.6 to 2.3 ± 0.8 mm, P < 0.001) and increase of Dice (from 0.89 ± 0.08 to 0.94 ± 0.4%, P < 0.001) before and after correction, respectively. The T1 map quality improved in 70 ± 0.3% of the motion‐affected maps after correction. Motion‐corrupted segments of the myocardium reduced from 21.8 to 8.5% (P < 0.001) after correction.ConclusionThe proposed method for nonrigid registration of T1‐weighted images allows T1 measurements in more myocardial segments by reducing motion‐induced T1 estimation errors in myocardial segments. Magn Reson Med 80:780–791, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
A framework to develop adapted treatment regimens to manage pediatric cancer in low‐ and middle‐income countries: The Pediatric Oncology in Developing Countries (PODC) Committee of the International Pediatric Oncology Society (SIOP)
Authors: Howard Scott C. , Davidson Alan , Luna‐Fineman Sandra , Israels Trijn , Chantada Guillermo , Lam Catherine G. , Hunger Stephen P. , Bailey Simon , Ribeiro Raul C. , Arora Ramandeep S. , Pedrosa Francisco , Harif Mhamed , Metzger Monika L. 
Published: January 2018

AbstractMany children with cancer in low‐ and middle‐income countries are treated in hospitals lacking key infrastructure, including diagnostic capabilities, imaging modalities, treatment components, supportive care, and personnel. Childhood cancer treatment regimens adapted to local conditions provide an opportunity to cure as many children as possible with the available resources, while working to improve services and supportive care. This paper from the Adapted Treatment Regimens Working Group of the Pediatric Oncology in Developing Countries committee of the International Society of Pediatric Oncology outlines the design, development, implementation, and evaluation of adapted regimens and specifies levels of services needed to deliver them.
Performance of SCAN density functional for a set of ionic liquid ion pairs
Authors: Karu Karl , Mišin Maksim , Ers Heigo , Sun Jianwei , Ivaništšev Vladislav 
Published: January 2018

AbstractComputational chemistry is a powerful tool for the discovery of novel materials. In particular, it is used to simulate ionic liquids in search of electrolytes for electrochemical applications. Herein, the choice of the computational method is not trivial, as it has to be both efficient and accurate. Density functional theory methods with appropriate corrections for the systematic weaknesses can give precision close to that of the post‐Hartree–Fock coupled cluster methods with a fraction of their cost. Thence, we have evaluated the performance of a recently developed nonempirical strongly constrained and appropriately normed (SCAN) density functional on electronic structure calculations of ionic liquid ion pairs. The performance of SCAN and other popular functionals (PBE, M06‐L, B2PLYP) among with Grimme's dispersion correction and Boys–Bernardi basis set superposition error correction was compared to DLPNO‐CCSD(T)/CBS. We show that SCAN reproduces coupled‐cluster results for describing the employed dataset of 48 ion pairs.
Solvent effects on differentiation of mouse brain tissue using laser microdissection ‘cut and drop’ sampling with direct mass spectral analysis
Authors: Cahill John F. , Kertesz Vilmos , Porta Tiffany , LeBlanc J.C. Yves , Heeren Ron M.A. , Van Berkel Gary J. 
Published: January 2018

RationaleLaser microdissection‐liquid vortex capture/electrospray ionization mass spectrometry (LMD‐LVC/ESI‐MS) has potential for on‐line classification of tissue but an investigation into what analytical conditions provide best spectral differentiation has not been conducted. The effects of solvent, ionization polarity, and spectral acquisition parameters on differentiation of mouse brain tissue regions are described.MethodsIndividual 40 × 40 μm microdissections from cortex, white, grey, granular, and nucleus regions of mouse brain tissue were analyzed using different capture/ESI solvents, in positive and negative ion mode ESI, using time‐of‐flight (TOF)‐MS and sequential window acquisitions of all theoretical spectra (SWATH)‐MS (a permutation of tandem‐MS), and combinations thereof. Principal component analysis‐linear discriminant analysis (PCA‐LDA), applied to each mass spectral dataset, was used to determine the accuracy of differentiation of mouse brain tissue regions.ResultsMass spectral differences associated with capture/ESI solvent composition manifested as altered relative distributions of ions rather than the presence or absence of unique ions. In negative ion mode ESI, 80/20 (v/v) methanol/water yielded spectra with low signal/noise ratios relative to other solvents. PCA‐LDA models acquired using 90/10 (v/v) methanol/chloroform differentiated tissue regions with 100% accuracy while data collected using methanol misclassified some samples. The combination of SWATH‐MS and TOF‐MS data improved differentiation accuracy.ConclusionsCombined TOF‐MS and SWATH‐MS data differentiated white, grey, granular, and nucleus mouse tissue regions with greater accuracy than when solely using TOF‐MS data. Using 90/10 (v/v) methanol/chloroform, tissue regions were perfectly differentiated. These results will guide future studies looking to utilize the potential of LMD‐LVC/ESI‐MS for tissue and disease differentiation.
Synthesis of O‐Alkyl Alkylphosphonates via Hydrazine Mediated Partial Dealkylation of Phosphonate Diesters
Authors: Pardasani Deepak , Purohit Ajay , Kumar Ajeet , Tak Vijay , Goud D Raghavender , Gupta Arvind K. , Dubey Devendra K. 
Published: January 2018

AbstractSimple and straightforward method for the partial dealkylation of phosphonate esters is reported for the preparation of alkyl alkylphosphonates. Hydrazine hydrate is used under solvent free conditions to generate nearly quantitative yields of the acids in reasonable time. The reactions are conducted under ambient conditions without any catalyst or specialized equipment.
Porous Biomaterials via Side Chain‐Side Chain Interactions of Tyrosine Analogue of Pyridine Carboxamides
Authors: Tiwari Priyanka , Biswas Soumava , Verma Ritu , Sharma Ankita , Dutt Konar Anita 
Published: January 2018

AbstractPeptide based porous materials have attracted recent attention due to their environmentally benign nature. In an attempt to artificially imitate their importance, we have synthesized a set of three pseudopeptides comprising of a single pyridinedicarboxylic acid and a flexible dipeptidyl fragment H‐Xaa‐Yaa‐OMe (Xaa: L‐Ala, Yaa: mABA, pseudopeptide I ; Xaa: L‐Tyr, Yaa : mABA, pseudopeptide II; Xaa: L‐Ile, Yaa : Tyr, pseudopeptide III, mABA: meta amino benzoic acid ) using Boc chemistry. Our experimental analysis using X‐ray crystallography illustrates that pseudopeptide I–III displays supramolecular preference for double helices, characterestic of pyridine carboxamides, employing intermolecular H‐bonding and π‐π analogy. To date, literature documentation reveals that the reported double helical motifs have been modulated either by utilizing DNA conjugates or rigid templates, mainly governed by the concept of backbone‐backbone interaction. However, to the best of our knowledge these novel examples represent one of the very few reports of the architectural design solely nucleated by ecofriendly amino acids driven by sidechain ‐ sidechain correspondence, exhibiting significant difference in self‐assembling properties. Our UV/Visible, concentration dependant NMR experiment and Circular Dichroism measurement complies with the solid state conformational analysis. Interestingly, the double helical ensemble of pseudopeptide II (Tyr analogue bearing additional H‐bonding ability of phenolic OH) unveil fifteen times more N2 sorption (65 cc/g) than pseudopeptide I (Ala, devoid of additional H‐bonding ability) (4.5 cc/g), furthermore emphasizing the dominant role of side chain ‐ side chain interaction in porous biomaterial design. Besides our findings also spotlights the relevance of positional displacement of Tyr‐mABA fragment in controlling gas adsorption properties (pseudopeptide III; 5 cc/g). Our morphological analysis using FE‐SEM inaddition supports our experimental observation.
Morphology‐Driven Sensitivity Enhancement of MEH‐PPV Langmuir‐Blodgett Films on Plastic Substrates for NO2 Gas
Authors: Saxena Vibha , Chandran Sridevi , Choudhury Sipra , Singh Ajay , Ganapathi Kailasa , Kaur Manmeet , Tanvi  , Muthe Kunal P. , Gadkari Sanjay C. 
Published: January 2018

Abstract3D ordered Langmuir–Blodgett (LB) films of poly[2‐methoxy‐5‐ (2‐ethylhexyloxy)‐1, 4‐phenylenevinylene] (MEH‐PPV) were prepared via thermal annealing. Surface properties of MEH‐PPV prepared by using different solvents, through both LB and Spin Casting techniques, were examined at the air‐water interface by measuring surface pressure‐area isotherms and Brewster Angle Microscopy. Dramatically different surface morphologies were obtained for spin cast and LB films, both prepared by using different solvents. The annealed LB films showed enhanced NO2 gas sensing as compared to spin cast films. Spectroscopy and morphological analyses reveal that enhanced response of LB films toward NO2 gas is due to their 3D ordered morphology, preferred molecular orientation for in‐plane transport as well as higher surface area.
Morphology‐Controllable Preparation of CeO2 Materials for CO2 Adsorption
Authors: Zhao Bosheng , Sha Feng , Ma Liang , Du Hong , Qiao Xianshu , Zhang Jianbin 
Published: January 2018

AbstractThree morphology controllable CeO2 crystals were successfully synthesized via the thermal decomposition of CeCO3OH precursors, which was newly prepared on the basis of a simple and template‐free hydrothermal reaction between CeCl3 solution and a CO2‐storage material (CO2SM). In the formation processes of three CeCO3OH precursors, the CO2SM could be newly used as raw material and structural director. Meanwhile, the results presented the wider pH values (5.24‐8.35) for isoelectric point of CeCO3OH precursors. After the CeCO3OH precursors were isolated, the filtrate could be recycled to absorb CO2 again and prepare CeCO3OH precursors with two special polymorphs. Three as‐prepared CeO2 crystals by annealing three CeCO3OH precursors at 500 °C for 4 h showed the maximum adsorption amounts of 23.5, 18.7 and 20.5 mg/g for 8% CO2/N2 mixture. As a result, this work provided the wider pH values for isoelectric point of CeCO3OH precursors and a simple method for the preparation of CeO2 crystals with a short time and CO2 adsorption properties.
Transcutaneous Laryngeal Ultrasonography for Laryngeal Immobility Diagnosis in Patients with Voice Disorders After Thyroid/Parathyroid Surgery
Authors: Lazard Diane S. , Bergeret‐Cassagne Héloïse , Lefort Muriel , Leenhardt Laurence , Russ Gilles , Frouin Frédérique , Trésallet Christophe 
Published: January 2018

AbstractBackgroundTranscutaneous laryngeal ultrasonography (TLUS) was recently developed to assess recurrent nerve palsy after thyroid/parathyroid surgery, with variable rates of efficiency. The aim of the current study was to evaluate this technique using subjective estimation and post‐processing quantitative data.
MethodsFifty subjects presenting with a recurrent nerve palsy and 50 “controls” presenting with voice, swallowing, or breathing disorders following thyroid/parathyroid surgery were prospectively included. All of them underwent a flexible laryngoscopy, considered the gold standard, and a ten‐second TLUS clip within the 10 days following surgery. In addition to the subjective interpretation of vocal fold motion, two quantitative criteria taking into account motion symmetry (symmetry index, SI) and amplitude (mobility index) of the two hemi‐larynges were defined on TLUS acquisitions in adduction and abduction.
ResultsThe subjective interpretation provided a sensitivity of 100% and a specificity of 96%, compared to the gold standard. The quantitative criteria provided a sensitivity and specificity of both 82%, when based on SI solely. When combining SI and mobility index, the sensitivity reached 94%, but the specificity fell to 66%.ConclusionsVisual assessment of recurrent nerve palsy using TLUS after thyroid/parathyroid surgery appeared a high sensitive and specific test compared to flexible laryngoscopy. Quantitative criteria are promising and need to be refined to better describe the whole TLUS video clip.