bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRTC2 promotes non‐small cell lung cancer A549 migration and invasion in vitro
Authors: Shi Shaoqing , Luo Wei , Zhang Rui , Wang Chu , Zheng Yuanyuan , Song Yunhua , Wang Rongchun , Zhang Liyan , Zhang Lihua , Li Weimin , Luo Zhuang 
Published: November 2017

Background
CRTC2 is highly expressed in lung cancer and contributes to lung cancer pathogenesis; however, whether CRTC2 promotes lung cancer metastasis remains unknown. In the present study, we investigated the role of CRTC2 in lung cancer metastasis in vitro.Methods
CRTC2 stable knockdown of lung cancer cell A549 was generated with small hairpin RNA and confirmed by quantitative reverse transcription‐PCR and Western blot. Wound healing and invasion transwell assays were performed to explore migration and invasion activity, and Western blot was conducted to detect the expression of related proteins.ResultsSuppression of CRTC2 significantly inhibited A549 cell migration and invasion in vitro. Mechanistic studies showed that knockdown of CRTC2 greatly downregulated MMP2 and MMP9 expression. CRTC2 silencing remarkably suppressed epithelial‐mesenchymal transition by modulating the expression of E‐cadherin and vimentin. Furthermore, suppression of CRTC2 expression significantly reduced MAPK/c‐Jun N‐terminal kinase activity.Conclusion
CRTC2 may promote A549 migration and invasion by modulation of c‐Jun N‐terminal kinase‐mediated epithelial‐mesenchymal transition and matrix metalloproteinase expression.
Adaptive parental effects and how to estimate them: A comment to Bonduriansky and Crean
Authors: Engqvist Leif , Reinhold Klaus 
Published: November 2017

Abstract

To fully understand the evolution of adaptive parental effects we need to reliably estimate their magnitude.

Recently, we (Engqvist & Reinhold, 2016, Methods in Ecology and Evolution, 7, 1482) highlighted some important problems when estimating the magnitude of anticipatory parental effects in the so‐called match/mismatch experiments. As the signature of such parental effects is a statistical interaction between parental environment and offspring environment, it will be difficult to disentangle these effects from other effects depending on a combination of parental and offspring environment, such as context‐dependent silver‐spoon (= condition‐transfer) effects.

In a recent article, Bonduriansky and Crean (2017, Methods in Ecology and Evolution) suggested to manipulate environmental quality on a continuous scale and using a geometric framework as a way out of this dilemma.

Here, we highlight and discuss the benefits and potential drawbacks of the suggested method. We conclude that using this approach, one may extract more detailed information but unfortunately, it will not resolve the interpretive ambiguity inherent in this experimental design.
V1b Receptor Antagonist SSR149415 and Naltrexone Synergistically Decrease Excessive Alcohol Drinking in Male and Female Mice
Authors: Zhou Yan , Rubinstein Marcelo , Low Malcolm J. , Kreek Mary Jeanne 
Published: November 2017

BackgroundA recent clinical trial found that pharmacological blockade of V1b receptors reduces alcohol relapse in alcohol‐dependent patients. SSR149415 is a selective V1b receptor antagonist that has potential for development as an alcohol dependency treatment. In this study, we investigated whether SSR149415 alone or in combination with the mu‐opioid receptor (MOP‐r) antagonist naltrexone (NTN) would alter excessive alcohol drinking in mice.MethodsBoth sexes of C57BL/6J (B6) mice were subjected to a chronic intermittent access (IA) drinking paradigm (2‐bottle choice, 24‐hour access every other day) for 3 weeks. Sucrose and saccharin drinking were used as controls for alcohol‐specific drug effects. Neuronal proopiomelanocortin (POMC) enhancer (nPE) knockout mice with hypothalamic‐specific loss of POMC (including beta‐endorphin, the main endogenous ligand of MOP‐r) were used as a genetic control for the effects of NTN.ResultsAcute administration of SSR149415 (1 to 30 mg/kg) reduced alcohol intake and preference in a dose‐dependent manner in both male and female B6 mice after IA. To investigate potential synergistic effects between NTN and SSR149415, we tested 6 different combination doses of SSR149415 and NTN, and found that a combination of SSR149415 (3 mg/kg) and NTN (1 mg/kg) reduced alcohol intake profoundly at doses lower than the individual effective doses in both sexes of B6 mice. We confirmed the effect of SSR149415 on reducing alcohol intake in nPE−/− male mice, consistent with independent mechanisms by which SSR149415 and NTN decrease alcohol drinking.ConclusionsThe combination of V1b antagonist SSR149415 with NTN at individual subthreshold doses shows potential in alcoholism treatment, possibly with less adverse effects.
Inhibition of IAP (inhibitor of apoptosis) proteins represses inflammatory status via nuclear factor‐kappa B pathway in murine endometriosis lesions
Authors: Taniguchi Fuminori , Uegaki Takashi , Nakamura Kazuomi , Mon Khine Yin , Harada Takashi , Ohbayashi Tetsuya , Harada Tasuku 
Published: November 2017

ProblemHow is the role of inhibitor of apoptosis proteins (IAPs) in the development of murine endometriosis lesions?Method of studyBALB/c female mice (n = 36) were used for the murine endometriosis model. Endometriotic lesions were surgically induced in mice by transplanting mouse uterine tissue. After 4 weeks of IAP antagonist (BV6) treatment, the expression of inflammatory factors in the implants was evaluated using real‐time RT‐PCR. Inflammatory state, angiogenic activity, and nuclear factor‐kappa B (NF‐κB) activation were assessed by immunohistochemical staining.ResultsThe number, size, and level of inflammatory cytokines (Vegf, Il‐6, Ccl‐2, Lif) gene expression in the murine endometriosis‐like lesions were reduced by BV6 treatment. BV6 repressed the intensity and rate of positive cells of CD3, F4/80, and PECAM immunostaining; in addition, the expression of NF‐κB p65 and phospho‐NF‐κB p65 was also attenuated.ConclusionInhibitor of apoptosis proteins antagonist represses the inflammation status of murine endometriosis‐like lesions viaNF‐κB pathway. IAPs may be a novel therapeutic target for endometriosis.
Medroxyprogesterone acetate‐treated human, primary endometrial epithelial cells reveal unique gene expression signature linked to innate immunity and HIV‐1 susceptibility
Authors: Woods Matthew W. , Zahoor Muhammad Atif , Dizzell Sara , Verschoor Chris P. , Kaushic Charu 
Published: November 2017

ProblemMedroxyprogesterone acetate (MPA), a progestin‐based hormonal contraceptive designed to mimic progesterone, has been linked to increased human immunodeficiency virus (HIV‐1) susceptibility. Genital epithelial cells (GECs) form the mucosal lining of the female genital tract (FGT) and provide the first line of protection against HIV‐1. The impact of endogenous sex hormones or MPA on the gene expression profile of GECs has not been comprehensively documented.Method of studyUsing microarray analysis, we characterized the transcriptional profile of primary endometrial epithelial cells grown in physiological levels of E2, P4, and MPA.ResultsEach hormone treatment altered the gene expression profile of GECs in a unique manner. Interestingly, although MPA is a progestogen, the gene expression profile induced by it was distinct from P4. MPA increased gene expression of genes related to inflammation and cholesterol synthesis linked to innate immunity and HIV‐1 susceptibility.ConclusionThe analysis of gene expression profiles provides insights into the effects of sex hormones and MPA on GECs and allows us to posit possible mechanisms of the MPA‐mediated increase in HIV‐1 acquisition.
National study shows that abusive head trauma mortality in Sweden was at least 10 times lower than in other Western countries
Authors: Andersson Jacob , Thiblin Ingemar 
Published: November 2017

AbstractAimThe validity of the diagnostic criteria for abusive head trauma (AHT) and its attributes has been widely debated. This national study investigated the possibility of false‐positive and false‐negative cases of fatal AHT in Sweden.MethodThis was a retrospective evaluation of the records of 733 deceased infants up to the age of 365 days who were examined during 1994–2013 at the six forensic medicine departments. All the records were scrutinised for possible cases of AHT.ResultsWe included 12 cases, of which eight had been diagnosed as AHT. Of these 12 infants, eight had a concomitant disease or perinatal illness, five were born prematurely and three were twins. Figures from other Western countries would suggest 6–7 deaths per 100,000 per year in Sweden, but in reality, there was a maximum possible incidence of 0.6 per 100,000 infants per year.ConclusionThe risk of unreported fatal AHT in Sweden was low, and there may have been cases misdiagnosed as AHT. The at least 10 times lower incidence than has been reported from other Western countries, raises the question if previously reported higher incidences of fatal AHT have been exaggerated.
Letter: questions regarding the diagnostic performance of serum assays for atrophic gastritis—Authors' reply
Authors: Zagari R. M. , Greenwood D. C. 
Published: November 2017

Linked ContentThis article is linked to Zagari et al and Graham papers. To view these articles visit https://doi.org/10.1111/apt.14248 and https://doi.org/10.1111/apt.14339.
Angiotensin receptor blocker use and gastro‐oesophageal cancer survival: a population‐based cohort study
Authors: Busby J. , McMenamin Ú. , Spence A. , Johnston B. T. , Hughes C. , Cardwell C. R. 
Published: November 2017

SummaryBackgroundAngiotensin receptor blockers (ARBs; including candesartan, losartan, olmesartan and valsartan) are widely used to treat hypertension, heart failure and diabetic neuropathy. There is considerable pre‐clinical evidence that ARBs can reduce cancer progression, particularly for gastric cancer. Despite this, epidemiological studies have yet to assess the impact of ARB use on gastro‐oesophageal cancer survival.AimTo investigate the association between post‐diagnosis ARB use and gastro‐oesophageal cancer survival.MethodsWe selected a cohort of patients with newly‐diagnosed gastro‐oesophageal cancer between 1998 and 2012 from English cancer registries. We linked to prescription and clinical records from the Clinical Practice Research Datalink, and to death records from the Office for National Statistics. We used time‐dependant Cox‐regression models to calculate hazard ratios (HRs) comparing gastro‐oesophageal cancer‐specific mortality between post‐diagnosis ARB users and non‐users, after adjusting for demographics, comorbidities and post‐diagnosis aspirin or statin use.ResultsOur cohort included 5124 gastro‐oesophageal cancer patients, of which 360 used ARBs, and 3345 died due to their gastro‐oesophageal cancer during follow‐up. After adjustment, ARB users had moderately lower risk of gastro‐oesophageal cancer mortality than the non‐users (HR = 0.83, 95% CI 0.71‐0.98). There was evidence of a dose–response relationship with the lowest HRs observed among patients receiving at least 2 years of prescriptions (HR = 0.42, 95% CI 0.25‐0.72).ConclusionsIn this large population‐based gastro‐oesophageal cancer cohort, we found moderately reduced cancer‐specific mortality among ARB users. However, confirmation in further independent epidemiological studies with sufficient staging information is required.
Mothering as Laboring: Navigating Cultural Discourses of Mothering as Marriage‐Labor Immigrants in Korea
Authors: Kim‐Bossard MinSoo 
Published: November 2017

AbstractThis paper examines the ways in which marriage‐labor immigrants manage, facilitate, and fulfill discourses about mothering in Korea by giving birth to and raising children. Through a series of semi‐structured ethnographic interviews, “go‐along” interviews, and fieldwork with marriage‐labor immigrants in Korea in 2013, 2014, and 2015, I investigated the emergent tension and anxiety around the recent demographic changes in Korea and the subsequent impact on discourses about mothering. This paper concludes by exploring the implications of how the reproductive and affective labor of marriage‐labor immigrants functions in contemporary Korea to facilitate cultural discourses about gender, lineage, and national identity.
Using learning analytics to scale the provision of personalised feedback
Authors: Pardo Abelardo , Jovanovic Jelena , Dawson Shane , Gašević Dragan , Mirriahi Negin 
Published: November 2017

AbstractThere is little debate regarding the importance of student feedback for improving the learning process. However, there remain significant workload barriers for instructors that impede their capacity to provide timely and meaningful feedback. The increasing role technology is playing in the education space may provide novel solutions to this impediment. As students interact with the various learning technologies in their course of study, they create digital traces that can be captured and analysed. These digital traces form the new kind of data that are frequently used in learning analytics to develop actionable recommendations that can support student learning. This paper explores the use of such analytics to address the challenges impeding the capacity of instructors to provide personalised feedback at scale. The case study reported in the paper showed how the approach was associated with a positive impact on student perception of feedback quality and on academic achievement. The study was conducted with first year undergraduate engineering students enrolled in a computer systems course with a blended learning design across three consecutive years (N2013 = 290, N2014 = 316 and N2015 = 415).
Beurteilung der Lebensqualität bei Patienten mit nicht‐melanozytärem Hautkrebs – psychometrische Validierung des EORTC QLQ‐C30‐Fragebogens
Authors: Müller Karolina , Karrer Sigrid , Szeimies Rolf‐Markus , Steinbauer Julia , Kohl Elisabeth , Steinbauer Dominik , Zeman Florian , Berneburg Mark , Koller Michael 
Published: November 2017

ZusammenfassungHintergrundNicht‐melanozytärer Hautkrebs (NMSC, nonmelanoma skin cancer) ist eine chronische und mitunter schwierig zu behandelnde Erkrankung, die die Lebensqualität (LQ) beeinträchtigt. Die vorliegende Studie wurde durchgeführt um zu prüfen, ob der Kernfragebogen Quality of Life Questionnaire ‐ Cancer (QLQ‐C30) der European Organization for Research and Treatment of Cancer (EORTC) ein geeignetes Instrument für die Beurteilung der LQ bei NMSC‐Patienten ist.Patienten und MethodenZur Bestimmung der psychometrischen Eigenschaften des Fragebogens wurden der QLQ‐C30 und der Dermatology Life Quality Index (DLQI) an 172 Patienten der Klinik und Poliklinik für Dermatologie des Universitätsklinikums Regensburg, Deutschland, ausgegeben.ErgebnisseDie interne Konsistenz aller Multi‐Item‐Skalen (außer einer) war akzeptabel, wobei Cronbachs Alpha‐Koeffizient zwischen 0,71 bis 0,93 lag. Die angenommene Skalenstruktur wurde durch Korrelationen zwischen Items und Skalen sowie Korrelationen zwischen Skalen innerhalb des QLQ‐C30 bestätigt. Verwandte Skalen des QLQ‐C30 und des DLQI korrelierten signifikant und belegten damit die Konstruktvalidität. Gleichzeitig wies der Anteil substanzieller Korrelationen (6 % ≥ 0,40) darauf hin, dass mit den beiden Fragebogen unterschiedliche Aspekte der LQ beurteilt werden. Der QLQ‐C30 differenzierte signifikant zwischen Patienten mit verschiedenen Erkrankungsgraden. Eine schwere Erkrankung ging mit stärkeren Beeinträchtigungen der Körperlichen, Rollen‐ und Kognitiven Funktion einher (p ≤ 0,030).SchlussfolgerungenDiese Ergebnisse bestätigen, dass der QLQ‐C30 ein geeignetes Instrument für die Beurteilung der LQ bei NMSC‐Patienten darstellt.
Association between diabetes mellitus and olfactory dysfunction: current perspectives and future directions
Authors: Zaghloul H. , Pallayova M. , Al‐Nuaimi O. , Hovis K. R. , Taheri S. 
Published: November 2017

AbstractThe increasing global prevalence of diabetes mellitus presents a significant challenge to healthcare systems today. Although diabetic retinopathy, nephropathy and neuropathy are well‐established complications of diabetes, there is a paucity of research examining the impact of dysglycaemia on the olfactory system. Olfaction is an important sense, playing a role in the safety, nutrition and quality of life of an individual, but its importance is often overlooked when compared with the other senses. As a result, olfactory dysfunction is often underdiagnosed. The present review article aims to present and discuss the available evidence on the relationship between diabetes and olfaction. It also explores the associations between olfactory dysfunction and diabetes complications that could explain the underlying pathogenesis. Finally, it summarizes the putative pathological mechanisms underlying olfactory dysfunction in diabetes that require further investigation.
Standards for data acquisition and software‐based analysis of in vivo electroencephalography recordings from animals. A TASK1‐WG5 report of the AES/ILAE Translational Task Force of the ILAE
Authors: Moyer Jason T. , Gnatkovsky Vadym , Ono Tomonori , Otáhal Jakub , Wagenaar Joost , Stacey William C. , Noebels Jeffrey , Ikeda Akio , Staley Kevin , de Curtis Marco , Litt Brian , Galanopoulou Aristea S. 
Published: November 2017

SummaryElectroencephalography (EEG)—the direct recording of the electrical activity of populations of neurons—is a tremendously important tool for diagnosing, treating, and researching epilepsy. Although standard procedures for recording and analyzing human EEG exist and are broadly accepted, there are no such standards for research in animal models of seizures and epilepsy—recording montages, acquisition systems, and processing algorithms may differ substantially among investigators and laboratories. The lack of standard procedures for acquiring and analyzing EEG from animal models of epilepsy hinders the interpretation of experimental results and reduces the ability of the scientific community to efficiently translate new experimental findings into clinical practice. Accordingly, the intention of this report is twofold: (1) to review current techniques for the collection and software‐based analysis of neural field recordings in animal models of epilepsy, and (2) to offer pertinent standards and reporting guidelines for this research. Specifically, we review current techniques for signal acquisition, signal conditioning, signal processing, data storage, and data sharing, and include applicable recommendations to standardize collection and reporting. We close with a discussion of challenges and future opportunities, and include a supplemental report of currently available acquisition systems and analysis tools. This work represents a collaboration on behalf of the American Epilepsy Society/International League Against Epilepsy (AES/ILAE) Translational Task Force (TASK1‐Workgroup 5), and is part of a larger effort to harmonize video‐EEG interpretation and analysis methods across studies using in vivo and in vitro seizure and epilepsy models.
Effect of talc and titania on the microstructure and mechanical properties of alumina ceramics
Authors: Yu Hui , Xu Zhihao , Wei Ziye , Chen Yongjun , Li Jianbao , Luo Lijie 
Published: November 2017

AbstractAlumina (Al2O3) ceramics were prepared with nucleating agent of titania (TiO2) and fluxing agent of talc by the normal pressure sintering method. The effects of TiO2 and talc on phase composition, microstructure, and mechanical properties of Al2O3 ceramics, as well the strengthening and toughening mechanism were investigated. It was found that the introduction of TiO2 could be favorable for mullite formation and the improved densification of ceramics, resulting in improved mechanical properties of ceramics as well. Ceramics possess the best comprehensive mechanical performance with fracture toughness of 3.69 MPa·m1/2 and flexural strength of 255.9 MPa when 4 wt.% TiO2 and 15 wt.% talc were added. Mineral bridges and nanocrystallites were found in the glass‐ceramics, which caused the deflection of cracks so that it strengthened the grain boundary and improved the mechanical properties of ceramics.
Effect of annealing on the functionality of Bambara groundnut (Vigna subterranea) starch–palmitic acid complex
Authors: Oyeyinka Samson A. , Adegoke Rukayat , Oyeyinka Adewumi T. , Salami Khadijat O. , Olagunju Omotola F. , Kolawole Fausat L. , Joseph John K. , Bolarinwa Islamiyat F. 
Published: November 2017

SummaryBambara groundnut is an underutilised African leguminous crop. This study investigated the effect of annealing on the complexing ability and functionality of Bambara groundnut starch with palmitic acid. Corn starch was included as the reference. Annealing created cracks and pores on the surface of Bambara groundnut and corn starches, respectively. Bambara groundnut starch had significantly higher amylose content, higher peak and final viscosities than corn starch. The peak viscosities of native Bambara groundnut and corn starches significantly reduced with palmitic acid addition. Greater reduction in peak viscosities was observed when the annealed starches were complexed with palmitic acid, suggesting that more palmitic acid was complexed after annealing. This was confirmed by XRD peaks and melting enthalpies. Pasting of native Bambara groundnut and corn starches with palmitic acid resulted in the formation of type I V‐amylose complexes, while type II complexes were formed from annealed starches pasted with palmitic acid.
Voluntary Work‐related Technology Use during Non‐work Time: A Narrative Synthesis of Empirical Research and Research Agenda
Authors: Schlachter Svenja , McDowall Almuth , Cropley Mark , Inceoglu Ilke 
Published: November 2017

AbstractThe Internet and mobilization of information and communication technologies (ICTs) have made non‐manual work increasingly portable and remotely accessible. As a result, a considerable number of employees use their ICTs to engage in work‐related tasks during designated non‐work time, even without contractual obligation. However, existing research on such voluntary work‐related ICT use remains fragmented and spread across disciplines. The authors conducted a narrative review of 56 studies to identify themes in existing research, synthesize the evidence base and identify gaps in understanding. They identify five themes: (1) Social‐normative organizational context, (2) Job‐related characteristics and work processes, (3) Person characteristics, (4) Designated non‐work time and well‐being, and (5) Empowerment/Enslavement Paradox. A conceptual model of voluntary ICT use is developed by integrating the identified themes with existing organizational research, outlining the relationships between the identified themes and voluntary ICT use. The discussion emphasizes the need for more conceptual clarity on voluntary ICT use and related constructs, and for the integration of different disciplines and methodological approaches to advance knowledge in the field. The authors further identify person‐centred research as a critical future avenue to explore different ICT user types. Additionally, more research into the mechanisms and moderating influences regarding voluntary ICT use and its outcomes is considered advisable to advance knowledge on the Empowerment/Enslavement Paradox and its potential resolution. The paper concludes with preliminary implications to inform practice, addressing the need for employers to provide control over voluntary ICT use and employees enacting this control.