bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
Authors: Liu Junhui , Sebastià Cristina , Jové-Juncà Teodor , Quintanilla Raquel , González-Rodríguez Olga , Passols Magí , Castelló Anna , Sánchez Armand , Ballester Maria , Folch Josep M. 
Published: September 2024

Abstract
Background
The composition and distribution of fatty acids (FA) are important factors determining the quality, flavor, and nutrient value of meat. In addition, FAs synthesized in the body participate in energy metabolism and are involved in different regulatory pathways in the form of signaling molecules or by acting as agonist or antagonist ligands of different nuclear receptors. Finally, synthesis and catabolism of FAs affect adaptive immunity by regulating lymphocyte metabolism. The present study performed genome-wide association studies using FA profiles of blood, liver, backfat and muscle from 432 commercial Duroc pigs.

Results
Twenty-five genomic regions located on 15 Sus scrofa chromosomes (SSC) were detected. Annotation of the quantitative trait locus (QTL) regions identified 49 lipid metabolism-related candidate genes. Among these QTLs, four were identified in more than one tissue. The ratio of C20:4n-6/C20:3n-6 was associated with the region on SSC2 at 7.56–14.26 Mb for backfat, liver, and muscle. Members of the fatty acid desaturase gene cluster (FADS1, FADS2, and FADS3) are the most promising candidate genes in this region. Two QTL regions on SSC14 (103.81–115.64 Mb and 100.91–128.14 Mb) were identified for FA desaturation in backfat and muscle. In addition, two separate regions on SSC9 at 0 – 14.55 Mb and on SSC12 at 0–1.91 Mb were both associated with the same multiple FA traits for backfat, with candidate genes involved in de novo FA synthesis and triacylglycerol (TAG) metabolism, such as DGAT2 and FASN. The ratio C20:0/C18:0 was associated with the region on SSC5 at 64.84–78.32 Mb for backfat. Furthermore, the association of the C16:0 content with the region at 118.92–123.95 Mb on SSC4 was blood specific. Finally, candidate genes involved in de novo lipogenesis regulate T cell differentiation and promote the generation of palmitoleate, an adipokine that alleviates inflammation.

Conclusions
Several SNPs and candidate genes were associated with lipid metabolism in blood, liver, backfat, and muscle. These results contribute to elucidating the molecular mechanisms implicated in the determination of the FA profile in different pig tissues and can be useful in selection programs that aim to improve health and energy metabolism in pigs.
A million-cow genome-wide association study of productive life in U.S. Holstein cows
Authors: Liang Zuoxiang , Prakapenka Dzianis , Zaabza Hafedh B. , VanRaden Paul M. , Van Tassell Curtis P. , Da Yang 
Published: September 2024

Abstract
Background
Productive life (PL) of a cow is the time the cow remains in the milking herd from first calving to exit from the herd due to culling or death and is an important economic trait in U.S. Holstein cattle. The large samples of Holstein genomic evaluation data that have become available recently provided unprecedented statistical power to identify genetic factors affecting PL in Holstein cows using the approach of genome-wide association study (GWAS).

Methods
The GWAS analysis used 1,103,641 Holstein cows with phenotypic observations on PL and genotypes of 75,282 single nucleotide polymorphism (SNP) markers. The statistical tests and estimation of SNP additive and dominance effects used the approximate generalized least squares method implemented by the EPISNPmpi computer program.

Results
The GWAS detected 5390 significant additive effects of PL distributed over all 29 autosomes and the X–Y nonrecombining region of the X chromosome (Chr31). Two chromosome regions had the most significant and largest cluster of additive effects, the SLC4A4-GC-NPFFR2 (SGN) region of Chr06 with pleiotropic effects for PL, fertility, somatic cell score and milk yield; and the 32–52 Mb region of Chr10 with peak effects for PL in or near RASGRP1 with many important immunity functions. The dominance tests detected 38 significant dominance effects including 12 dominance effects with sharply negative homozygous recessive genotypes on Chr18, Chr05, Chr23 and Chr24.

Conclusions
The GWAS results showed that highly significant genetic effects for PL were in chromosome regions known to have highly significant effects for fertility and health and a chromosome region with multiple genes with reproductive and immunity functions. SNPs with rare but sharply negative homozygous recessive genotypes for PL existed and should be used for eliminating heifers carrying those homozygous recessive genotypes.
Inspiring nurses’ sustainability mindset: Exploring the Mediating Role of Organizational Culture on the relationship between Pro-social Leader behaviors and nurses’ sustainability consciousness
Authors: Atalla Amal Diab Ghanem , Mostafa Wafaa Hassan , Ali Mohamed Saad Saleh 
Published: September 2024

Abstract
Background
Since nurses are at the frontline of healthcare delivery, their actions and understanding of the environment have a big impact on how long healthcare systems can last. It is essential to comprehend the elements that impact nurses’ sustainability consciousness to encourage ecologically conscious actions in the healthcare industry.

Aim
This study aimed to explore the relationship between pro-social leader behaviors and nurses’ sustainability consciousness and testify to the mediating role of organizational culture in this relationship.

Design
A cross-sectional descriptive correlational design by STROBE criteria was used.

Methods and tools
An approach to a judgmental non-probability sampling technique was employed to obtain data from 350 nurses in an Egyptian hospital. Three measurement surveys were employed: Organizational Culture Survey, Prosociality Scale, and, Sustainability Consciousness Questionnaire (SCQ-S). Relationships were shown using structural equation modeling and descriptive and inferential statistics.

Results
53.4% of nurses have high perceptions of organizational culture, and the majority of nurses (85.7%) have high perceptions of prosocial leader behaviors. Furthermore, 60.9% of nurses have high perceptions of sustainability consciousness. Additionally, Prosocial leader behaviors positively correlated with organizational culture (r = 0.129) and nurses’ sustainability consciousness (r = 0.274). The indirect effect of prosocial leader behaviors on nurses’ sustainability consciousness through organizational culture is calculated by multiplying the coefficients of both direct effects (0.129 * 0.159 = 0.811). This means that for each unit increase in prosocial leader behaviors, we would expect a 0.811 unit increase in nurses’ sustainability consciousness through the mediating effect of organizational culture. The model appears to match the data well based on the model fit parameters (CFI = 1.000, IFI = 1.000, RMSEA = 0.114).

Conclusions
The study highlights the impact of pro-social leader behaviors on nurses’ sustainability consciousness through the organizational culture as a mediating factor. Nursing Implications: Findings from this research can promote environmental stewardship and sustainable practices in the healthcare sector by illuminating the elements that can encourage and support a sustainability-oriented mindset among nurses. To promote a more sustainable future for the nursing profession, the findings can guide activities in nursing education, corporate culture transformation, and leadership development.
Incidence and characteristics of prehospital fatalities from haemorrhage in Sweden: a nationwide observational study
Authors: von Olnhausen Oliver , Wladis Andreas , Bäckström Denise 
Published: September 2024

Abstract
Background
Haemorrhage is a leading cause of preventable mortality in high-income countries and emergency management presents unique challenges in the prehospital setting. The study aimed to determine incidence and characteristics of fatalities from prehospital haemorrhage in Sweden.

Methods
A nationwide retrospective cohort study 2012–2021 was conducted using data from the Swedish National Board of Health and Welfare. Prehospital fatality from haemorrhage was defined as a cause of death related to haemorrhage (Appendix 1) without a hospital admission on the same day. Primary outcome was age-standardized mortality rate per 100,000 inhabitants.

Results
A total of 9801 prehospital fatalities from haemorrhage were identified. Annual age-standardized mortality rate decreased from 10.97 to 8.18 per 100,000 population (coefficient =  − 0.28, r2 = 0.85, p =  < 0.001). Trauma was the most common cause (3512, 35.83%) with intentional self-harm (X60–X84), transport accidents (V01–V99) and assault (X85–Y09) being the most common mechanisms of injury. Traumatic fatalities were younger and a larger proportion were male compared to non-traumatic causes (p < 0.001). Overall median Charlson Comorbidity Index (Quan) was 0 [0–2] with a lower index noted for traumatic causes (p < 0.001). Trauma resulted in a median of 26.1 [3.65–49.22] years of life lost per patient compared to 0 [0–3.65] for non-traumatic causes (p < 0.001). Regional variations in mortality rate were observed with lower population density correlating with higher mortality rate (ρ =  − 0.64, p = 0.002).

Conclusions
Prehospital mortality from haemorrhage decreased between 2012 and 2021. Trauma was the most common cause which resulted in many years of life lost in a population with a low burden of comorbidities. There were considerable regional differences with low population density associated with higher mortality rate from prehospital haemorrhage.
Side differences in upper quarter mobility/stability are not related to serve velocity in tennis players with different levels of training experience
Authors: Lambrich Johanna , Panzer Stefan , Muehlbauer Thomas 
Published: September 2024

Abstract
Objective
Tennis is characterised by repetitive serves and strokes predominately performed with one arm. This can lead to differences in upper quarter mobility/stability between the stroke and non-stroke arm, which could even enlarge with increasing training experience and negatively affect serve velocity. Thus, we determined side differences (i.e., limb symmetry index) in upper quarter mobility/stability and their association with flat and slice serve velocity in advanced (ITN ≤ 4) female and male tennis players (N = 42, mean age = 23.9 ± 9.3 years) with different levels of training experience (< 2 years: n = 14, 2–5 years: n = 17, 6–8 years: n = 11).

Results
Y Balance Test-Upper Quarter (YBT-UQ) side difference (i.e., composite score) and performances (i.e., medial reach) were largest in players with the lowest level of training experience (i.e., < 2 years). Further, YBT-UQ performances (i.e., medial reach and composite score) but not side differences were significantly correlated with flat and slice serve velocity, particularly in less experienced players. Our results suggest that significant side differences in upper quarter mobility/stability occur in less experienced players (indicative of increased injury risk) but they are not related to tennis-specific performance (i.e., serve velocity).
Single-cell sequencing suggests a conserved function of Hedgehog-signalling in spider eye development
Authors: Medina‑Jiménez Brenda I. , Budd Graham E. , Pechmann Matthias , Posnien Nico , Janssen Ralf 
Published: September 2024

Abstract
Background
Spiders evolved different types of eyes, a pair of primary eyes that are usually forward pointing, and three pairs of secondary eyes that are typically situated more posterior and lateral on the spider’s head. The best understanding of arthropod eye development comes from the vinegar fly Drosophila melanogaster, the main arthropod model organism, that also evolved different types of eyes, the larval eyes and the ocelli and compound eyes of the imago. The gene regulatory networks that underlie eye development in this species are well investigated revealing a conserved core network, but also show several differences between the different types of eyes. Recent candidate gene approaches identified a number of conserved genes in arthropod eye development, but also revealed crucial differences including the apparent lack of some key factors in some groups of arthropods, including spiders.

Results
Here, we re-analysed our published scRNA sequencing data and found potential key regulators of spider eye development that were previously overlooked. Unlike earlier research on this topic, our new data suggest that Hedgehog (Hh)-signalling is involved in eye development in the spider Parasteatoda tepidariorum. By investigating embryonic gene expression in representatives of all main groups of spiders, we demonstrate that this involvement is conserved in spiders. Additionally, we identified genes that are expressed in the developing eyes of spiders, but that have not been studied in this context before.

Conclusion
Our data show that single-cell sequencing represents a powerful method to gain deeper insight into gene regulatory networks that underlie the development of lineage-specific organs such as the derived set of eyes in spiders. Overall, we gained deeper insight into spider eye development, as well as the evolution of arthropod visual system formation.
The national educational panel study (NEPS) and methodological innovations in longitudinal large-scale assessments
Authors: Kutscher Tanja , Sengewald Marie-Ann , Gnambs Timo , Carstensen Claus H. , Aßmann Christian 
Published: September 2024

AbstractThis editorial introduces a special issue of Large-Scale Assessments in Education (LSAE) that addresses key challenges in analyzing longitudinal data from large-scale studies. These challenges include ensuring fair measurement across time, developing common metrics, and correcting for measurement errors. The special issue highlights recent methodological innovations, particularly for studies like the National Education Panel Study (NEPS), providing approaches for improving the accuracy and robustness of longitudinal educational research. The papers in this issue present advances in methods for estimating trends, incorporating background information, and analyzing longitudinal relationships between constructs. Innovative approaches such as Bayesian modeling for borrowing historical information, continuous-time models for capturing developmental trends, and plausible value estimation provide practical solutions for researchers working with complex longitudinal data. In addition, the issue presents new software tools that facilitate the implementation of these advanced methodologies. Together, these papers contribute to both the theory and practice of educational assessment and provide valuable insights for those working with longitudinal data in national and international panel studies.
Short-term prediction of geomagnetic secular variation with an echo state network
Authors: Nakano Shin’ya , Sato Sho , Toh Hiroaki 
Published: September 2024

AbstractA technique for predicting the secular variation (SV) of the geomagnetic field based on the echo state network (ESN) model is proposed. SV is controlled by the geodynamo process in the Earth’s outer core, and modeling its nonlinear behaviors can be challenging. This study employs an ESN to represent the short-term temporal evolution of the geomagnetic field on the Earth’s surface. The hindcast results demonstrate that the ESN enables us to predict SV for a duration of several years with satisfactory accuracy. It is also found that the prediction is robust to the length of the the training data period. This suggests that the recent features of the SV are important for short-term prediction and that the ESN effectively learns these features.
Graphical Abstract
Characteristics of the seismogenic zone in an arc-continent collision belt: insights from seismic b values in Eastern Taiwan
Authors: Yeh Yu-Lien , Huang Bor-Yu , Wen Strong 
Published: September 2024

AbstractEastern Taiwan overlies a suture zone between the Eurasian Plate and the Philippine Sea Plate and is characterized by frequent earthquakes, often resulting in significant disasters. Notably, the region exhibits characteristics such as a high frequency of earthquakes and a short recurrence period for intense seismic events. While prior research has explored seismic b values across various periods in Taiwan, detailed investigations of the b value in the eastern region are lacking. This study employs the earthquake catalog compiled by the Taiwan Central Weather Administration to analyze spatial–temporal variations in b values in eastern Taiwan. The analysis encompasses seismic events occurring between January 1996 and June 2019. The seismic catalog is divided into three distinct time periods related to large seismic events: period I, 1996–2003 (the Chengkung earthquake); period II, 2003–2013 (the Ruisui earthquake); and period III, 2013–2019 (the Hualien earthquake). Our results indicate that most seismic events with a magnitude greater than 6 are associated with low b values. The overall b value increases during period II and then decreases substantially during period III. Although the estimated b values changed slightly, but the uncertainty in b values remained stable in this study. The epicenters of large earthquakes often overlap with areas with lower b values, especially in plate suture zones, which means that areas with lower b values usually have a higher probability of larger earthquakes. Given the extremely high potential for a catastrophic earthquake, mitigating measures should be adopted at all times.
Graphical Abstract
Expounding the application of nano and micro silica as a complementary additive in metakaolin phosphate geopolymer for ceramic applications—micro and nanoscale structural investigation
Authors: Vanitha N. , Thanigaiselvan Rithikaa , Manivannan M. , Jeyalakshmi R. , Megha S. N. , Kesavan M. 
Published: September 2024

AbstractMetakaolin phosphate geopolymers comprising poly-phospho-siloxo units are known for their structural performance, additionally advancing their microstructure with the transformation of crystalline berlinite phases at elevated temperatures. The intrinsic reaction of Al of metakaolin in the acid exploited, but the reaction of secondary silica phases is limitedly known. Metakaolin as a primary precursor (M) with the addition of 2% and 5% of nano silica (MS2 and MS5) and micro silica (MM2 and MM5) cast using 8-M phosphoric acid was cured at 80 °C. To enhance the utilization of geopolymer in any high-temperature applications, the structural transformations were studied after heating to various temperatures (200, 400, 600 and 800 °C) by XRD, Raman, TGA-DTA, SEM, XPS, FTIR and MAS-NMR. Sample M attained a strength of 46.2 MPa enhanced to 63.6 MPa in MS5 and 54.2 MPa in MM5. This can be ascribed from the transformation of Si–O–Al–O–Si into Si–O–Al–O–P from Raman bands. Comparing the chemical shift of Al (IV) to control, micro silica addition shifts the signal to a lower field (53 to 50 ppm) related to the increase of the number of Al-connected Si to give a tougher network. Nanoindentation is visualized from hardness and elasticity, and the corresponding values are 1.4 to 2.1 GPa and 0.8 to 1.4 GPa for loads ranging from 20 to 100 mN in silica-reinforced samples that are much higher than M. The micro and macro hardness is due to the reinforcement of quartz in micro silica around the gel. TGA-DTA showed that the reduction of mass loss is as high as 25.4% in control whereas 17.2% in MS5 and 15.8% in the MM5. Further, shrinkage rate in MS5 and MM5 was as low as − 1.1% and − 0.8% throughout the temperature range from 25 to 1000 °C and thus provides the way of use of nano and micro form of silica for better thermal resistance.
Graphical Abstract
Three new reduced forms of synthesized Schiff bases as potent anti-corrosion inhibitors for carbon steel in artificial seawater
Authors: Jafari Hojat , Ameri Elham , Soltanolkottabi Fariba , Berisha Avni 
Published: September 2024

AbstractAs part of the development of a new organic entity, we synthesized three new reduced forms of Schiff bases named 2,2’-(((2,2-dimethylpropane-1,3-diyl)bis(azanediyl)bis(methylene)disphenol (I1), 4,4’-(((2,2-dimethylpropane-1,3-diyl)bis (azanediyl)bis(methylene)bis(2-methoxyphenol) (I2), and 6,6’-(((2,2-dimethylpropane-1,3-diyl) bis(azanediyl)bis(methylene)bis(2-methoxyphenol) (I3). In order to develop new organic ligands to inhibit steel corrosion in 1M HCl solution, various electrochemical methods, such as electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP), along with surface visualization through atomic force microscopy (AFM), were employed. PDP results revealed excellent inhibition by compound I3 (71%) at a concentration of 1 mg/L. These findings were supported by the observation of a protective layer formation during prolonged immersion of steel in a corrosive solution, with or without inhibitors. In addition to gaining insights into the interaction mechanism and adsorption mode, density functional theory, Monte Carlo, and molecular dynamic simulations were conducted, revealing valuable information about the interaction of the inhibitors with the steel surface. Average surface roughness (Ra) values obtained for the artificial seawater in the absence and presence of inhibitor are 887 nm for blank, 195 nm for I1, 158 nm for I2, and 105 nm for I3.
Peripheral and central regulation of neuro–immune crosstalk
Authors: Izumi Mayuko , Nakanishi Yoshimitsu , Kang Sujin , Kumanogoh Atsushi 
Published: September 2024

AbstractThe neural and immune systems sense and respond to external stimuli to maintain tissue homeostasis. These systems do not function independently but rather interact with each other to effectively exert biological actions and prevent disease pathogenesis, such as metabolic, inflammatory, and infectious disorders. Mutual communication between these systems is also affected by tissue niche-specific signals that reflect the tissue environment. However, the regulatory mechanisms underlying these interactions are not completely understood. In addition to the peripheral regulation of neuro–immune crosstalk, recent studies have reported that the central nervous system plays essential roles in the regulation of systemic neuro–immune interactions. In this review, we provide an overview of the molecular basis of peripheral and systemic neuro–immune crosstalk and explore how these multilayered interactions are maintained.