bluuebunny/crossref_metadata_2025_split huggingface dataset:
Piezo type mechanosensitive ion channel component 1 functions as a regulator of the cell fate determination of mesenchymal stem cells
Authors: Sugimoto Asuna , Miyazaki Aya , Kawarabayashi Keita , Shono Masayuki , Akazawa Yuki , Hasegawa Tomokazu , Ueda-Yamaguchi Kimiko , Kitamura Takamasa , Yoshizaki Keigo , Fukumoto Satoshi , Iwamoto Tsutomu 
Published: December 2017

AbstractThe extracellular environment regulates the dynamic behaviors of cells. However, the effects of hydrostatic pressure (HP) on cell fate determination of mesenchymal stem cells (MSCs) are not clearly understood. Here, we established a cell culture chamber to control HP. Using this system, we found that the promotion of osteogenic differentiation by HP is depend on bone morphogenetic protein 2 (BMP2) expression regulated by Piezo type mechanosensitive ion channel component 1 (PIEZO1) in MSCs. The PIEZO1 was expressed and induced after HP loading in primary MSCs and MSC lines, UE7T-13 and SDP11. HP and Yoda1, an activator of PIEZO1, promoted BMP2 expression and osteoblast differentiation, whereas inhibits adipocyte differentiation. Conversely, PIEZO1 inhibition reduced osteoblast differentiation and BMP2 expression. Furthermore, Blocking of BMP2 function by noggin inhibits HP induced osteogenic maker genes expression. In addition, in an in vivo model of medaka with HP loading, HP promoted caudal fin ray development whereas inhibition of piezo1 using GsMTx4 suppressed its development. Thus, our results suggested that PIEZO1 is responsible for HP and could functions as a factor for cell fate determination of MSCs by regulating BMP2 expression.
Characterization of a major QTL for manganese accumulation in rice grain
Authors: Liu Chaolei , Chen Guang , Li Yuanyuan , Peng Youlin , Zhang Anpeng , Hong Kai , Jiang Hongzhen , Ruan Banpu , Zhang Bin , Yang Shenglong , Gao Zhenyu , Qian Qian 
Published: December 2017

AbstractSome diets lack sufficient manganese (Mn), an essential mineral. Increasing Mn in grain by biofortification could prevent Mn deficiency, but may increase levels of the toxic element cadmium (Cd). Here, we investigated Mn in rice (Oryza sativa) grains in recombinant inbred lines (RILs) from the cross of 93–11 (low grain Mn) with PA64s (high grain Mn). Quantitative trait locus (QTL) analysis to identify loci controlling grain Mn identified a major QTL, qGMN7.1, on the short arm of chromosome 7; qGMN7.1 explained 15.6% and 22.8% of the phenotypic variation in the RIL populations grown in two distinct environments. We validated the QTL with a chromosome segment substitution line (CSSL), CSSL-qGMN7.1, in the 93–11 background harboring qGMN7.1 from PA64s. Compared to 93–11, CSSL-qGMN7.1 grain had increased Mn and decreased Cd concentrations; CSSL-qGMN7.1 roots also showed enhanced Mn uptake. Fine mapping delimited qGMN7.1 to a 49.3-kb region containing OsNRAMP5, a gene responsible for Mn and Cd uptake. Sequence variations in the OsNRAMP5 promoter caused changes in its transcript level, and in grain Mn levels. Our study thus cloned a major QTL for grain Mn concentration in rice, and identified materials for breeding rice for high Mn and low Cd concentrations in the grain.
Chemical Adsorption and Physical Confinement of Polysulfides with the Janus-faced Interlayer for High-performance Lithium-Sulfur Batteries
Authors: Chiochan Poramane , Kaewruang Siriroong , Phattharasupakun Nutthaphon , Wutthiprom Juthaporn , Maihom Thana , Limtrakul Jumras , Nagarkar Sanjog S. , Horike Satoshi , Sawangphruk Montree 
Published: December 2017

AbstractWe design the Janus-like interlayer with two different functional faces for suppressing the shuttle of soluble lithium polysulfides (LPSs) in lithium-sulfur batteries (LSBs). At the front face, the conductive functionalized carbon fiber paper (f-CFP) having oxygen-containing groups i.e., -OH and -COOH on its surface was placed face to face with the sulfur cathode serving as the first barrier accommodating the volume expansion during cycling process and the oxygen-containing groups can also adsorb the soluble LPSs via lithium bonds. At the back face, a crystalline coordination network of [Zn(H2PO4)2(TzH)2]n (ZnPTz) was coated on the back side of f-CFP serving as the second barrier retarding the left LPSs passing through the front face via both physical confinement and chemical adsorption (i.e. Li bonding). The LSB using the Janus-like interlayer exhibits a high reversible discharge capacity of 1,416 mAh g−1 at 0.1C with a low capacity fading of 0.05% per cycle, 92% capacity retention after 200 cycles and ca. 100% coulombic efficiency. The fully charged LSB cell can practically supply electricity to a spinning motor with a nominal voltage of 3.0 V for 28 min demonstrating many potential applications.
Photoluminescence Study of the Photoinduced Phase Separation in Mixed-Halide Hybrid Perovskite CH3NH3Pb(BrxI1−x)3 Crystals Synthesized via a Solvothermal Method
Authors: Zhang Baohua , Guo Fuqiang , Xue Junjun , Yang Lianhong , Zhao Yafei , Ge Mei , Cai Qing , Liu Bin , Xie Zili , Chen Dunjun , Lu Hai , Zhang Rong , Zheng Youdou 
Published: December 2017

AbstractWe systematically synthesized mixed-halide hybrid perovskite CH3NH3Pb(BrxI1−x)3 (0 ≤ x ≤ 1) crystals in the full composition range by a solvothermal method. The as-synthesized crystals retained cuboid shapes, and the crystalline structure transitioned from the tetragonal phase to the cubic phase with an increasing Br-ion content. The photoluminescence (PL) of CH3NH3Pb(BrxI1−x)3 crystals exhibited a continuous variation from red (768 nm) to green (549 nm) with increasing the volume ratio of HBr (VHBr%), corresponding to a variation in the bandgap from 1.61 eV to 2.26 eV. Moreover, the bandgap of the crystals changed nonlinearly as a quadratic function of x with a bowing parameter of 0.53 eV. Notably, the CH3NH3Pb(BrxI1−x)3 (0.4 ≤ x ≤ 0.6) crystals exhibited obvious phase separation by prolonged illumination. The cause for the phase separation was attributed to the formation of small clusters enriched in lower-band-gap, iodide-rich and higher-band-gap, bromide-rich domains, which induced localized strain to promote halide phase separation. We also clarified the relationship between the PL features and the band structures of the crystals.
Challenges in the determination of the interstellar flow longitude from the pickup ion cutoff
Authors: Taut A. , Berger L. , Möbius E. , Drews C. , Heidrich-Meisner V. , Keilbach D. , Lee M. A. , Wimmer-Schweingruber R. F. 
Published: December 2017

Context. The interstellar flow longitude corresponds to the Sun’s direction of movement relative to the local interstellar medium. Thus, it constitutes a fundamental parameter for our understanding of the heliosphere and, in particular, its interaction with its surroundings, which is currently investigated by the Interstellar Boundary EXplorer (IBEX). One possibility to derive this parameter is based on pickup ions (PUIs) that are former neutral ions that have been ionized in the inner heliosphere. The neutrals enter the heliosphere as an interstellar wind from the direction of the Sun’s movement against the partially ionized interstellar medium. PUIs carry information about the spatial variation of their neutral parent population (density and flow vector field) in their velocity distribution function. From the symmetry of the longitudinal flow velocity distribution, the interstellar flow longitude can be derived.Aim. The aim of this paper is to identify and eliminate systematic errors that are connected to this approach of measuring the interstellar flow longitude; we want to minimize any systematic influences on the result of this analysis and give a reasonable estimate for the uncertainty.Methods. We use He+ data measured by the PLAsma and SupraThermal Ion Composition (PLASTIC) sensor on the Solar TErrestrial RElations Observatory Ahead (STEREO A) spacecraft. We analyze a recent approach, identify sources of systematic errors, and propose solutions to eliminate them. Furthermore, a method is introduced to estimate the error associated with this approach. Additionally, we investigate how the selection of interplanetary magnetic field angles, which is closely connected to the pickup ion velocity distribution function, affects the result for the interstellar flow longitude.Results. We find that the revised analysis used to address part of the expected systematic effects obtains significantly different results than presented in the previous study. In particular, the derived uncertainties are considerably larger. Furthermore, an unexpected systematic trend of the resulting interstellar flow longitude with the selection of interplanetary magnetic field orientation is uncovered.
Clinical Outcome of Urgent Thoracotomy in Patients with Penetrating and Blunt Chest Trauma: A Retrospective Survey
Authors: Refaely Yael , Koyfman Leonid , Friger Michael , Ruderman Leonid , Saleh Mahmud , Sahar Gidon , Shaked Gad , Klein Moti , Brotfain Evgeni 
Published: December 2017

Introduction In contrast to an emergency department of thoracotomy (EDT), an urgent thoracotomy (UT) is defined as a surgical thoracic intervention performed in the operating room within the first 48 hours of the patient's intensive care unit (ICU) stay. The factors affecting survival after UT are not fully understood. In this study, we retrospectively analyzed the clinical data and outcome of patients with blunt and penetrating chest injuries who underwent UT.
Methods All adult patients who had blunt or penetrating chest trauma and who underwent UT, were included in the study. All data were collected from the patients' hospital and ICU records. Forty-five patients with thoracic injuries who underwent UT during the first 48 hours of ICU stay were analyzed. Of these, 25 had penetrating chest injuries, and 20 had blunt thoracic injuries. Of the penetrating injuries, 16 were stab wounds, and 9 were gunshot wounds.
Results Overall ICU mortality was 29% (n = 13) and was significantly higher in the blunt chest trauma group than in the penetrating trauma group (45% vs 16%; p = 0.04). Lung parenchyma injuries (lacerations and contusions) were the most common intraoperative findings in both groups. The following independent predictors of in-hospital mortality were found: an Injury Severity Score (ISS) of >40; an Acute Physiology and Chronic Evaluation II (APACHE II) score of >30; prolonged duration of UT; low body temperature on admission to the ED; abnormal arterial blood lactate, bicarbonate, and pH at the end of UT; and use of vasopressors during the first 24 hours of ICU stay.
Conclusion Mortality after UT was higher in patients with blunt chest trauma. The UT should be performed in both penetrating and blunt chest trauma as quickly as possible and should be limited to damage control. It also emerges that acidosis and hypothermia in chest trauma patients need to be treated extremely aggressively before, during, and after UT.
Extracorporeal Membrane Oxygenation in Postcardiotomy Pediatric Patients—15 Years of Experience Outside Europe and North America
Authors: Ismail Mohamed , Hamouda Tamer , Shaikh Rafik , Mahmoud Alaa , Shihata Mohammad , Alradi Osman , Jamjoom Ahmed , ElMahrouk Ahmed 
Published: December 2017

Background The increasing complexity of congenital cardiac surgery has resulted in the increased use of extracorporeal membrane oxygenation (ECMO) support for children who cannot be weaned from cardiopulmonary bypass. The purpose of this research was to assess the mortality and morbidity in children requiring ECMO support after the repair of congenital heart defects (CHDs).
Methods The hospital records of all patients with CHD who required ECMO after a cardiac surgical procedure between January 2001 and December 2016 were retrospectively reviewed. Various outcomes were reported and tested for any association with hospital death.
Results A total of 113 children required ECMO for cardiopulmonary support after congenital cardiac surgery; 88 (77.9%) were placed on ECMO in the operating room. Median age of the patients was 3 months (range, 4 days–15 years) and median weight was 3.5 kg (range, 2.2–42.5). Forty-two (37.2%) survived to hospital discharge. In children with single-ventricle physiology, survival to discharge was 37.3% (19/51 patients) and for biventricular physiology, it was 37.1% (23/62 patients). Univariate analysis revealed number of days on ECMO support, renal failure, and stroke as risk factors for hospital mortality, while age and cross-clamp time were found to be statistically nonsignificant.
Conclusion Satisfactory results can be achieved in pediatric patients by using ECMO support for postoperative cardiac and pulmonary failure refractory to medical management. Prolonged ECMO support, renal failure, and stroke are risk of mortality.
Apixaban-Calibrated Anti-FXa Activity in Relation to Outcome Events and Clinical Characteristics in Patients with Atrial Fibrillation: Results from the AVERROES Trial
Authors: Bhagirath Vinai , Eikelboom John , Hirsh Jack , Coppens Michiel , Ginsberg Jeffrey , Vanassche Thomas , Yuan Fei , Chan Noel , Yusuf Salim , Connolly Stuart 
Published: December 2017

Background In patients with nonvalvular atrial fibrillation (AF), apixaban is given in doses of 5 or 2.5 mg twice daily, according to clinical characteristics. The usual on-treatment range of apixaban drug levels, as determined by apixaban-calibrated anti-factor Xa (anti-Xa) activity, has previously been measured in small cohorts; however, the association between anti-Xa activity and clinical outcomes and the predictors of variability in anti-Xa activity have not been well studied in the AF population.
Methods and Results Anti-Xa activity was measured before taking the morning dose, 3 months after enrollment in the AVERROES study using a calibrated anti-Xa assay (Rotachrom). Patients with two of the following criteria—age >80; weight <60 kg; or creatinine >133 μg/L—received 2.5 mg twice daily (n = 145), while all others received 5 mg twice daily (n = 2,247). A total of 2,392 patients were included, with median follow-up of 1.1 years. Median apixaban anti-Xa activity was 122 ng/mL (interquartile range [IQR]: 63–198 ng/mL) for the entire group; 99 ng/mL (IQR: 60–146 ng/mL) for the 2.5-mg group; and 125 ng/mL (IQR: 64–202 ng/mL) for the 5-mg group (p = 0.003). A relationship was evident between bleeding and anti-Xa activity (p = 0.01), which was driven by minor bleeding. No relationship was evident between major bleeding or stroke/systemic embolism and anti-Xa activity. In those receiving the 5-mg dose, estimated glomerular filtration rate, sex, and age had the strongest association with anti-Xa activity.
Conclusion There is considerable variability in anti-Xa activity among AF patients receiving apixaban. Rates of major bleeding and stroke/systemic embolism were low irrespective of anti-Xa activity.
Clinical Trial Registration ClinicalTrials.gov NCT00496769; https://clinicaltrials.gov/ct2/show/NCT00496769.
Einfluss der Synovialflüssigkeit auf die Schmiereigenschaften von Gelenkknorpel in vitro
Authors: Thier Steffen , Tonak Marcus 
Published: December 2017

ZusammenfassungDer hyaline Gelenkknorpel besitzt einzigartige tribologische Eigenschaften, die für die Reduktion von Verschleiß und Abrieb essenziell erscheinen. Er ist in der Lage, trotz durchschnittlich 1 – 4 Mill. Belastungszyklen pro Jahr mit Spitzenbelastungen bis 18 MPa seine Funktion jahrzehntelang aufrechtzuerhalten. Maßgeblich scheinen hierfür verschiedene Schmiermechanismen und deren synergistisches Zusammenspiel verantwortlich zu sein. Insbesondere bei der Grenzflächenschmierung sind die physiologischen Schmiermoleküle („Biolubrikanzien“) der Synovialflüssigkeit von übergeordneter Wichtigkeit. Sie gewährleisten in Abhängigkeit vom vorherrschenden Schmiermechanismus eine nahezu friktionslose/kontaktlose Reibung der Gelenkoberflächen. Zur Testung dieser Eigenschaften und Abhängigkeiten wurden im Laufe der letzten Jahrzehnte verschiedene tribologische „In-vitro“-Prüfsysteme entwickelt. Hierdurch wurden Voraussetzungen für die Grundlagenforschung der Arthrose und Entwicklung von Substanzen zur Viskosupplementation geschaffen. Aufgrund der Komplexität und Vielfalt der Prüfsysteme und Prüfbedingungen ist es schwierig, in diesem Zusammenhang den Überblick zu behalten. Ziel dieses Beitrags ist es, einen kursorischen Überblick über den Einfluss der Synovialflüssigkeit und ihrer Bestandteile auf die Schmierung von Gelenkknorpel unter In-vitro-Prüfbedingungen vorzustellen und verständlich zu machen.
Der zeitliche Verlauf der Visusentwicklung nach einer Injektion von Aflibercept oder Ranibizumab bei der neovaskulären altersbedingten Makuladegeneration
Authors: Wilke Robert , Finger Robert , Sachs Helmut 
Published: December 2017

Zusammenfassung
Hintergrund Die Behandlungswirklichkeit der neovaskulären altersbedingten Makuladegeneration (nvAMD) unterscheidet sich hinsichtlich Injektionsintervallen und langfristiger Visusstabilität von kontrollierten prospektiven Studien. Real-Life-Daten zeigen, dass in der klinischen Routine engmaschige Kontroll- und Wiederbehandlungsintervalle oft nicht eingehalten werden. Wir analysieren an einer Fallserie die tatsächlichen Intervalle für Kontrollen und Wiederbehandlung und untersuchen, wie sich verlängerte Intervalle unmittelbar auf den Visusverlauf auswirken und welche Konsequenzen dies für den langfristigen Visuserhalt hat.
Patienten/Material und Methoden Retrospektive Fallserie von 1324 Augen mit nvAMD, die über einen Beobachtungszeitraum von bis zu 3 Jahren 8150 Ranibizumab-Injektionen (nach PRN-Schema) bzw. 1725 Aflibercept-Injektionen (nach festem Schema) erhalten haben. Die Intervalle zwischen aufeinanderfolgenden Visiten, der Visusverlauf und die Anzahl der Injektionen im Therapieverlauf wurden ausgewertet.
Ergebnisse Die angestrebten Intervalle zwischen einzelnen Visiten von 4 bzw. 8 Wochen werden im PRN-Schema häufiger überschritten als im fixen Schema. Im Durchschnitt ist der maximale Visusanstieg nach einer Injektion nicht nach 4 Wochen, sondern erst nach etwa 6 – 8 Wochen erreicht. Ein statistisch signifikanter Unterschied zwischen Aflibercept und Ranibizumab wurde nicht gefunden. Die besten Visusergebnisse nach 1 und 2 Jahren werden mit einem durchschnittlichen Wiederbehandlungsintervall von 4 – 6 Wochen erreicht. Verlängerte Intervalle gehen mit schlechterem Langzeitvisus einher. Das fixe Schema zeigt auch im Therapieverlauf im 2. und 3. Jahr kürzere Re-Injektionsintervalle und bessere Visusergebnisse als das PRN-Schema.
Schlussfolgerungen Unsere Daten zeigen, dass engmaschige Nachkontrollen und kurze Behandlungsintervalle wichtig und wesentliche Einflussfaktoren für die Visusergebnisse sind. Insgesamt erscheint die Wahl eines Therapieschemas, das dies ermöglicht, wichtiger als die Wahl des Präparates selbst.
Lequemédiatif du français contemporain
Authors: Anscombre Jean-Claude 
Published: December 2017

RésuméThe French constructionheureusement queshows aquethat has been recently analyzed as a very specific relative pronoun, an evidentialque, which introduces a proposition considered as having been previously uttered. On the other hand, the pronounqui – apart from its common use as an anaphoric relative – had a specific function until the end of the 17th century: it could be used with no antecedent, and with an indefinite and suppositive value meaning ‘if one’. The first part of this study draws up a full list of both constructions in French. The second part of the study is mainly diachronic, and explores the origins of these two pronouns in classical Latin, as well as their evolution through Romance languages, namely French, Italian and Spanish.
Designing for sustainable outcomes: espousing behavioural change into co-production programmes
Authors: Mukherjee Ishani , Mukherjee Nilanjana 
Published: December 2017

AbstractThis paper uses a policy design perspective with which to examine the formulation of programmes that are based on the concept of co-production. In doing so, the paper reviews essential literature on policy design and co-production to identify that a limited focus on outcomes and specifically how behavioural change can make these outcomes sustainable represents a major gap in the current discussion of co-production. We firstly argue that in designing programmes involving co-production, outcomes need to be considered at the initial design stages where broad policy objectives are being defined. Secondly, we argue that for these outcomes to be sustainable, behavioural change on the part of policy targets needs to be an important objective of a coproduction programme. To illustrate our point, we use the example of rural sanitation programmes from three developing countries to specifically demonstrate how the absence or inclusion of behavioural change considerations in the early phases of policy design can elicit different levels of success in achieving desired policy outcomes.
Final report, on-going key comparison BIPM.QM-K1, ozone at ambient level, comparison with CMS-ITRI, June 2016
Authors: Viallon le , Idrees Faraz , Moussay Philippe , Wielgosz Robert , Lin Tsai-Yin , Norris James E , Hodges Joseph T 
Published: December 2017

As part of the on-going key comparison BIPM.QM-K1, a comparison has been performed between the ozone national standard of the ITRI Center for Measurement Standards (CMS-ITRI) and the common reference standard of the key comparison, maintained by the Bureau International des Poids et Mesures (BIPM), via a transfer standard maintained by the National Institute of Standards and Technology (NIST). The instruments have been compared over a nominal ozone amount-of-substance fraction range of 0 nmol/mol to 500 nmol/mol.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).