bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bronchial cell epigenetic aging in a human experimental study of short-term diesel and ozone exposures
Authors: Nwanaji-Enwerem Jamaji C , Bozack Anne K , Ward-Caviness Cavin , Diaz-Sanchez David , Devlin Robert B , Bind Marie-Abèle C , Cardenas Andres 
Published: September 2024

Abstract
Blood-based, observational, and cross-sectional epidemiological studies suggest that air pollutant exposures alter biological aging. In a single-blinded randomized crossover human experiment of 17 volunteers, we examined the effect of randomized 2-h controlled air pollution exposures on respiratory tissue epigenetic aging. Bronchial epithelial cell DNA methylation 24 h post-exposure was measured using the HumanMethylation450K BeadChip, and there was a minimum 2-week washout period between exposures. All 17 volunteers were exposed to ozone, but only 13 were exposed to diesel exhaust. Horvath DNAmAge [Pearson coefficient (r) = 0.64; median absolute error (MAE) = 2.7 years], GrimAge (r = 0.81; MAE = 13 years), and DNAm Telomere Length (DNAmTL) (r = −0.65) were strongly correlated with chronological age in this tissue. Compared to clean air, ozone exposure was associated with longer DNAmTL (median difference 0.11 kb, Fisher’s exact P-value = .036). This randomized trial suggests a weak relationship of ozone exposure with DNAmTL in target respiratory cells. Still, causal relationships with long-term exposures need to be evaluated.
DNA methylation correlates with transcriptional noise in response to elevated pCO2 in the eastern oyster (Crassostrea virginica)
Authors: Venkataraman Yaamini R , Huffmyer Ariana S , White Samuel J , Downey-Wall Alan , Ashey Jill , Becker Danielle M , Bengtsson Zachary , Putnam Hollie M , Strand Emma , Rodríguez-Casariego Javier A , Wanamaker Shelly A , Lotterhos Katie E , Roberts Steven B 
Published: September 2024

Abstract
Ocean acidification significantly affects marine calcifiers like oysters, warranting the study of molecular mechanisms like DNA methylation that contribute to adaptive plasticity in response to environmental change. However, a consensus has not been reached on the extent to which methylation modules gene expression, and in turn plasticity, in marine invertebrates. In this study, we investigated the impact of pCO2 on gene expression and DNA methylation in the eastern oyster, Crassostrea virginica. After a 30-day exposure to control (572 ppm) or elevated pCO2 (2827 ppm), whole-genome bisulfite sequencing (WGBS) and RNA-seq data were generated from adult female gonad tissue and male sperm samples. Although differentially methylated loci (DMLs) were identified in females (89) and males (2916), there were no differentially expressed genes and only one differentially expressed transcript in females. However, gene body methylation impacted other forms of gene activity in sperm, such as the maximum number of transcripts expressed per gene and changes in the predominant transcript expressed. Elevated pCO2 exposure increased gene expression variability (transcriptional noise) in males but decreased noise in females, suggesting a sex-specific role of methylation in gene expression regulation. Functional annotation of genes with changes in transcript-level expression or containing DMLs revealed several enriched biological processes potentially involved in elevated pCO2 response, including apoptotic pathways and signal transduction, as well as reproductive functions. Taken together, these results suggest that DNA methylation may regulate gene expression variability to maintain homeostasis in elevated pCO2 conditions and could play a key role in environmental resilience in marine invertebrates.
CMR native T1 and T2 mapping in Olympic athletes: the influence of sports discipline and sex
Authors: Prosperi Silvia , Monosilio Sara , Lemme Erika , Filomena Domenico , Penza Marco , Birtolo Lucia Ilaria , Mango Ruggiero , Di Gioia Giuseppe , Gualdi Gianfranco , Squeo Maria Rosaria , Pelliccia Antonio , Maestrini Viviana 
Published: September 2024

Abstract

Aims
Cardiac magnetic resonance (CMR) has a growing role in evaluating athletes’ hearts. Mapping techniques provide added value for tissue characterization, but data on athletes and sports disciplines are lacking. To describe native mapping values in a cohort of Olympic athletes and evaluate the influence of sports discipline and sex.

Methods and results
A group of 300 Olympic athletes (13% skill, 20% power, 25% mixed, 42% endurance, 58% male) with unremarkable cardiovascular screening and a control group of 42 sedentary subjects (52% male) underwent CMR without contrast administration. Athletes were divided based on sex and sports categories according to the ESC classification. Among athletes of different sports categories and controls, endurance presented the lowest value of T1 mapping (P &lt; 0.001). No differences in T2 mapping were observed (P = 0.472). Female athletes had higher values of T1 native myocardial mapping compared with males (P = 0.001), while there were no differences in T2 mapping (P = 0.817). Male athletes with higher left ventricular mass indexed (LV-Massi) had lower values of T1 mapping (P = 0.006) and slightly higher values of T2 mapping, even if not significant (P = 0.150). Female athletes with higher LV-Massi did not show significant differences in T1 and T2 mapping (P = 0.053 and P = 0.438).

Conclusion
T1 native myocardial mapping showed significant differences related to sports disciplines and gender. Athletes with the largest LV remodelling, mostly endurance and mixed, showed the lowest values of T1 mapping. Male athletes showed lower values of T1 mapping than females. No significant differences were observed in T2 mapping related to sports disciplines and gender.
Impact of implementing primary care-based medication for opioid use disorder on provider and staff perceptions
Authors: Mazzarelli Sara , Blewer Audrey L , Østbye Truls , Rhodes Katherine , Plasencia Gabriela , Hart Lauren , Sawin Gregory 
Published: September 2024

Abstract
Medication for opioid use disorder (MOUD) is the management of opioid use disorder (OUD) on an outpatient basis with buprenorphine or buprenorphine/naloxone (or methadone, which is limited to federally certified opioid treatment programs). Primary care practices are well poised to provide comprehensive care for patients with OUD, including provision of MOUD. The aim of this study was to assess provider and staff OUD attitudes and role perceptions before and after implementation of a MOUD clinical service line. A survey was distributed to evaluate attitudes and perceptions of patients with OUD and provision of MOUD among providers and staff in an academic family medicine clinic. Surveys were distributed in December 2020 (73% response rate), prior to a substance use disorder educational training and MOUD service line implementation, which provided patients with OUD both primary care services and management with buprenorphine/naloxone. A follow-up survey was distributed in February 2022 (69% response rate).Training and implementation of the MOUD service line demonstrated improvements in the domains of motivation (+0.63), attitudes (+0.32), satisfaction (+0.38), role support (+0.48), role adequacy (+0.39), and safety (+0.79) among surveyed participants. The change in satisfaction and safety domains was statistically significant (P &lt; .05). There was no change in the role legitimacy domain.Implementation of a primary care-based MOUD service line positively affected provider and staff motivation, attitudes, satisfaction, sense of safety, role support, and adequacy when working with patients with OUD. This highlights the benefits of MOUD-specific clinical support to optimize care delivery within primary care.
Agar lot-specific inhibition in the plating efficiency of yeast spores and cells
Authors: Protacio Reine U , Davidson Mari K , Malone Emory G , Helmlinger Dominique , Smith Jeremy R , Gibney Patrick A , Wahls Wayne P 
Published: September 2024

Abstract
The fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae are highly diverged (530 mya), single-celled, and model eukaryotic organisms. Scientists employ mating, meiosis, and the plating of ascospores and cells to generate strains with novel genotypes and to discover biological processes. Our 3 laboratories encountered independently sudden-onset, major impediments to such research. Spore suspensions and vegetative cells no longer plated effectively on minimal media. By systematically analyzing multiple different media components from multiple different suppliers, we identified the source of the problem. Specific lots of agar were toxic. We report that this sporadic toxicity affects independently the agar stocks of multiple vendors, has occurred repeatedly over at least 3 decades, and extends to species in highly diverged taxa. Interestingly, the inhibitory effects displayed variable penetrance and were attenuated on rich media. Consequently, quality control checks that use only rich media can provide false assurances on the quality of the agar. Lastly, we describe likely sources of the toxicity and we provide specific guidance for quality control measures that should be applied by all vendors as preconditions for their sale of agar.
Mistranslating tRNA variants have anticodon- and sex-specific impacts on Drosophila melanogaster
Authors: Isaacson Joshua R , Berg Matthew D , Jagiello Jessica , Yeung William , Charles Brendan , Villén Judit , Brandl Christopher J , Moehring Amanda J 
Published: September 2024

Abstract
Transfer RNAs (tRNAs) are vital in determining the specificity of translation. Mutations in tRNA genes can result in the misincorporation of amino acids into nascent polypeptides in a process known as mistranslation. Since mistranslation has different impacts, depending on the type of amino acid substitution, our goal here was to compare the impact of different mistranslating tRNASer variants on fly development, lifespan, and behaviour. We established two mistranslating fly lines, one with a tRNASer variant that misincorporates serine at valine codons (V→S) and the other that misincorporates serine at threonine codons (T→S). While both mistranslating tRNAs increased development time and developmental lethality, the severity of the impacts differed depending on amino acid substitution and sex. The V→S variant extended embryonic, larval, and pupal development whereas the T→S only extended larval and pupal development. Females, but not males, containing either mistranslating tRNA presented with significantly more anatomical deformities than controls. Since mistranslation disrupts cellular translation and proteostasis, we also tested the hypothesis that tRNA variants impact fly lifespan. Interestingly, mistranslating females experienced extended lifespan whereas mistranslating male lifespan was unaffected. Consistent with delayed neurodegeneration and beneficial effects of mistranslation, mistranslating flies from both sexes showed improved locomotion as they aged. The ability of mistranslating tRNA variants to have both positive and negative effects on fly physiology and behaviour has important implications for human health given the prevalence of tRNA variants in humans.
Genomic insights into endangerment and conservation of the garlic-fruit tree (Malania oleifera), a plant species with extremely small populations
Authors: Shen Yuanting , Tao Lidan , Zhang Rengang , Yao Gang , Zhou Minjie , Sun Weibang , Ma Yongpeng 
Published: September 2024

Abstract

Background
Advanced whole-genome sequencing techniques enable covering nearly all genome nucleotide variations and thus can provide deep insights into protecting endangered species. However, the use of genomic data to make conservation strategies is still rare, particularly for endangered plants. Here we performed comprehensive conservation genomic analysis for Malania oleifera, an endangered tree species with a high amount of nervonic acid. We used whole-genome resequencing data of 165 samples, covering 16 populations across the entire distribution range, to investigate the formation reasons of its extremely small population sizes and to evaluate the possible genomic offsets and changes of ecology niche suitability under future climate change.

Results
Although M. oleifera maintains relatively high genetic diversity among endangered woody plants (θπ = 3.87 × 10−3), high levels of inbreeding have been observed, which have reduced genetic diversity in 3 populations (JM, NP, and BM2) and caused the accumulation of deleterious mutations. Repeated bottleneck events, recent inbreeding (∼490 years ago), and anthropogenic disturbance to wild habitats have aggravated the fragmentation of M. oleifera and made it endangered. Due to the significant effect of higher average annual temperature, populations distributed in low altitude exhibit a greater genomic offset. Furthermore, ecological niche modeling shows the suitable habitats for M. oleifera will decrease by 71.15% and 98.79% in 2100 under scenarios SSP126 and SSP585, respectively.

Conclusions
The basic realizations concerning the threats to M. oleifera provide scientific foundation for defining management and adaptive units, as well as prioritizing populations for genetic rescue. Meanwhile, we highlight the importance of integrating genomic offset and ecological niche modeling to make targeted conservation actions under future climate change. Overall, our study provides a paradigm for genomics-directed conservation.
Gender-responsive health promotion for women: regulating the sociopolitical landscape of alcohol product marketing
Authors: Lunnay Belinda , Foley Kristen 
Published: September 2024

Abstract
This perspective piece calls for health promotion action to regulate alcohol product marketing targeting women and create environments where it is possible to mitigate the harms of alcohol and protect women’s health. Drawing on the Global Alcohol Action Plan in the context of the Australian National Women’s Health Strategy 2020–30, we consider critical actions for gender-responsive health promotion to protect women from the ways alcohol companies market their products utilizing women’s gendered social roles and entrenched stereotypes. We show how these subtle yet powerful gendered approaches to alcohol marketing have the potential for harm yet are not covered by the current mechanisms of the self-regulated Alcohol Beverages Advertising Code. We draw on the World Health Organization’s 2024 Framework on Gender-Responsive Approaches to the Acceptability Availability and Affordability of Alcohol and make a call to regulate alcohol marketing targeting women.
Not just new wine in old bottles: Seeing refugee law and human rights as entangled regimes
Authors: Scott Ford Sarah 
Published: September 2024

Abstract
This article re-examines the relationship between international human rights and refugee law as a story of mutual interaction, by harnessing and developing the nascent concept of legal entanglement. While entanglement serves as an alternative starting point to understand how legal regimes mutually affect each other, this new concept still lacks sufficient clarity for making sense of the interfaces and development of international law. This article proposes an analytical framework for international legal theory by outlining a model of the normative, interpretive, and socio-political drivers of entangled regimes. It argues that new norms, interpretations, and socio-political shifts explain the rapprochement and distancing between refugee law and human rights over time. Acknowledging the risk that entanglement framing may become altogether too encompassing, the proposed approach to entangled regimes allows for precision and nuance in the empirical tracing of international law.
Capacity Allocation in Distributed Wind Power Generation Hybrid Energy Storage Systems
Authors: Wang Yupeng , Fan Yuxing 
Published: September 2024

Abstract
The inherent variability and uncertainty of distributed wind power generation exert profound impact on the stability and equilibrium of power storage systems. In response to this challenge, we present a pioneering methodology for the allocation of capacities in the integration of wind power storage. Firstly, we introduce a meticulously designed uncertainty modeling technique aimed at optimizing wind power forecasting deviations, thus augmenting the controllability of distributed wind power variations. Subsequently, we establish a cutting-edge real-time dynamic optimization model for state of charge, which effectively mitigates the fluctuations associated with grid-connected wind power. Moreover, we employ a state-of-the-art distributed robust optimization algorithm to enhance the stability of the distributed wind power storage system. Through comprehensive simulation testing, our findings unequivocally demonstrate the efficacy of our approach in preserving a harmonious balance between wind power load and output demand, thereby assuring the unwavering operation of the entire system. Notably, our approach attains an exceptional capacity allocation efficiency of 91% in the rigorous wind power grid-smoothing test, outperforming comparable methodologies. Lastly, we proffer essential recommendations pertaining to attenuation optimization at the effective capacity level of the batteries, effectively safeguarding the long-term stability of the energy storage system.
Generative AI in orthopedics: an explainable deep few-shot image augmentation pipeline for plain knee radiographs and Kellgren-Lawrence grading
Authors: Littlefield Nickolas , Amirian Soheyla , Biehl Jacob , Andrews Edward G , Kann Michael , Myers Nicole , Reid Leah , Yates Adolph J , McGrory Brian J , Parmanto Bambang , Seyler Thorsten M , Plate Johannes F , Rashidi Hooman H , Tafti Ahmad P 
Published: September 2024

Abstract

Objectives
Recently, deep learning medical image analysis in orthopedics has become highly active. However, progress has been restricted by the absence of large-scale and standardized ground-truth images. To the best of our knowledge, this study is the first to propose an innovative solution, namely a deep few-shot image augmentation pipeline, that addresses this challenge by synthetically generating knee radiographs for training downstream tasks, with a specific focus on knee osteoarthritis Kellgren-Lawrence (KL) grading.

Materials and Methods
This study leverages a deep few-shot image augmentation pipeline to generate synthetic knee radiographs. Despite the limited availability of training samples, we demonstrate the capability of our proposed computational strategy to produce high-fidelity plain knee radiographs and use them to successfully train a KL grade classifier.

Results
Our experimental results showcase the effectiveness of the proposed computational pipeline. The generated synthetic radiographs exhibit remarkable fidelity, evidenced by the achieved average Frechet Inception Distance (FID) score of 26.33 for KL grading and 22.538 for bilateral knee radiographs. For KL grading classification, the classifier achieved a test Cohen’s Kappa and accuracy of 0.451 and 0.727, respectively. Our computational strategy also resulted in a publicly and freely available imaging dataset of 86 000 synthetic knee radiographs.

Conclusions
Our approach demonstrates the capability to produce top-notch synthetic knee radiographs and use them for KL grading classification, even when working with a constrained training dataset. The results obtained emphasize the effectiveness of the pipeline in augmenting datasets for knee osteoarthritis research, opening doors for broader applications in orthopedics, medical image analysis, and AI-powered diagnosis.
Reinvention mediates impacts of skin tone bias in algorithms: implications for technology diffusion
Authors: Overbye-Thompson Hannah , Hamilton Kristy A , Mastro Dana 
Published: September 2024

Abstract
Two studies examine how skin tone bias in image recognition algorithms impacts users’ adoption and usage of image recognition technology. We employed a diffusion of innovations framework to explore perceptions of compatibility, complexity, observability, relative advantage, and reinvention to determine their influence on participants' utilization of image recognition algorithms. Despite being more likely to encounter algorithm bias, individuals with darker skin tones perceived image recognition algorithms as having greater levels of compatibility and relative advantage, being more observable, and less complex and thus used them more extensively compared to those with lighter skin tones. Individuals with darker skin tones also displayed higher levels of reinvention behaviors, suggesting a potential adaptive response to counteract algorithm biases.
Phase II study of carboplatin plus weekly paclitaxel with bevacizumab for non-squamous, non-small cell lung cancer with idiopathic interstitial pneumonia (Hanshin Cancer Group IP002)
Authors: Katakami Nobuyuki , Nagata Kazuma , Nakakura Akiyoshi , Okamoto Tadashi , Kaneda Toshihiko , Oki Masahide , Watanabe Kana , Tokito Takaaki , Amano Yoshihiro , Tamiya Motohiro , Morita Satoshi , Hatachi Yukimasa 
Published: September 2024

Abstract

Background
There is an increased risk of acute exacerbation of idiopathic interstitial pneumonia when treating patients with advanced non-small cell lung cancer with idiopathic interstitial pneumonia. There is no standard optimal treatment regimen for patients with lung cancer complicated with idiopathic interstitial pneumonia. We aimed to evaluate the efficacy and safety of carboplatin (CBDCA), bevacizumab (Bmab) and weekly paclitaxel (PXT) in patients with idiopathic interstitial pneumonia.

Methods
This phase 2 study involved chemotherapy-naïve patients with advanced non-small cell lung cancer with idiopathic interstitial pneumonia. Patients received CBDCA (area under the curve: 5 on day 1), PXT (70 mg/m2 on days 1, 8 and 15) and Bmab (15 mg/kg on day 1) every 4 weeks. The primary endpoint was the overall response rate.

Results
Twenty-one patients were enrolled between January 2013 and October 2018 and received at least one course of the protocol treatment. The study was terminated before enrolling the planned number of patients because of poor accrual. The median patient age was 69 (range: 62–79) years, and 19 (90.5%) patients were men. The overall response rate was 61.9% (95% confidence interval [CI], 38.4–81.9), meeting the primary endpoint. The median progression-free survival, time to treatment failure, and overall survival were 9.69 (95% CI, 5.78–11.63), 8.21 (95% CI, 3.75–11.63) and 20.93 (95% CI, 13.17–29.83) months, respectively. There was no acute exacerbation or treatment-related death during protocol treatment.

Conclusion
The results indicate that patients with advanced non-squamous, non-small cell lung cancer with idiopathic interstitial pneumonia could be effectively and safely treated using a combination of CBDCA, PXT and Bmab.