bluuebunny/crossref_metadata_2025_split huggingface dataset:
First month prednisone dose predicts prednisone burden during the following 11 months: an observational study from the RELES cohort
Authors: Ruiz-Irastorza G , Garcia M , Espinosa G , Caminal L , Mitjavila F , González-León R , Sopeña B , Canora J , Villalba M V , Rodríguez-Carballeira M , López-Dupla J M , Callejas J L , Castro A , Tolosa C , Sánchez-García M E , Pérez-Conesa M , Navarrete-Navarrete N , Rodríguez A P , Herranz M T , Pallarés L 
Published: August 2016

AimTo study the influence of prednisone dose during the first month after systemic lupus erythematosus (SLE) diagnosis (prednisone-1) on glucocorticoid burden during the subsequent 11 months (prednisone-2–12).Methods223 patients from the Registro Español de Lupus Eritematoso Sistémico inception cohort were studied. The cumulative dose of prednisone-1 and prednisone-2–12 were calculated and recoded into a four-level categorical variable: no prednisone, low dose (up to 7.5 mg/day), medium dose (up to 30 mg/day) and high dose (over 30 mg/day). The association between the cumulative prednisone-1 and prednisone-2–12 doses was tested. We analysed whether the four-level prednisone-1 categorical variable was an independent predictor of an average dose >7.5 mg/day of prednisone-2–12. Adjusting variables included age, immunosuppressives, antimalarials, methyl-prednisolone pulses, lupus nephritis and baseline SLE Disease Activity Index (SLEDAI).ResultsWithin the first month, 113 patients (51%) did not receive any prednisone, 24 patients (11%) received average low doses, 46 patients (21%) received medium doses and 40 patients (18%) received high doses. There was a strong association between prednisone-1 and prednisone-2–12 dose categories (p<0.001). The cumulative prednisone-1 dose was directly associated with the cumulative prednisone-2–12 dose (p<0.001). Compared with patients on no prednisone, patients taking medium (adjusted OR 5.27, 95% CI 2.18 to 12.73) or high-dose prednisone-1 (adjusted OR 10.5, 95% CI 3.8 to 29.17) were more likely to receive prednisone-2–12 doses of >7.5 mg/day, while patients receiving low-dose prednisone-1 were not (adjusted OR 1.4, 95% CI 0. 0.38 to 5.2). If the analysis was restricted to the 158 patients with a baseline SLEDAI of ≥6, the model did not change.ConclusionThe dose of prednisone during the first month after the diagnosis of SLE is an independent predictor of prednisone burden during the following 11 months.
SURFACE FINISHING OF NANOSCRATCH PATTERNS ON COATED PYREX GLASSES USING MAF
Authors: LEE SEOUNG HWAN 
Published: August 2016

We investigate a surface finishing scheme fitted for AFM scratched nanopatterns on a coated surface using magnetic abrasive finishing (MAF). First, nanoscale patterning was carried out on IZO-coated Pyrex glass, with a specially-designed AFM machining setup. After AFM machining, nonuniform surface features such as pile-ups are generated, and the final shapes are often more complicated for coated materials. For this reason, MAF was implemented for the nanoscale removal of pile-up/debris of coated materials and design of experiment (DOE) was utilized to obtain optimal process conditions for the finishing process. The process was monitored using acoustic emission (AE) for coating-substrate (glass) boundary detection, as well for monitoring the polishing status/depth. Experimental results indicate the viability of the proposed scheme as a nanoscale selective finishing technique for various material setups.
Metastatic Progression of Prostate Cancer Is Mediated by Autonomous Binding of Galectin-4-O-Glycan to Cancer Cells
Authors: Tsai Chin-Hsien , Tzeng Sheue-Fen , Chao Tai-Kuang , Tsai Chia-Yun , Yang Yu-Chih , Lee Ming-Ting , Hwang Jiuan-Jiuan , Chou Yu-Ching , Tsai Mong-Hsun , Cha Tai-Lung , Hsiao Pei-Wen 
Published: August 2016

Abstract
Metastatic prostate cancer continues to pose a difficult therapeutic challenge. Prostate cancer progression is associated with aberrant O-glycosylation of cancer cell surface receptors, but the functional impact of such events is uncertain. Here we report spontaneous metastasis of human prostate cancer xenografts that express high levels of galectin-4 along with genetic signatures of EGFR-HER2 signaling and O-glycosylation. Galectin-4 expression in clinical specimens of prostate cancer correlated with poor patient survival. Galectin-4 binding to multiple receptor tyrosine kinases stimulated their autophosphorylation, activated expression of pERK, pAkt, fibronectin, and Twist1, and lowered expression of E-cadherin, thereby facilitating epithelial–mesenchymal transition, invasion, and metastasis. In vivo investigations established that galectin-4 expression enabled prostate cancer cells to repopulate tumors in orthotopic and heterotopic tissues. Notably, these effects of galectin-4 relied upon O-glycosylation mediated by C1GALT1, a galactosyltransferase implicated in other cancers. Parallel changes in galectin-4 and O-glycosylation triggered aberrant receptor signaling and more aggressive invasive character in prostate cancer cells, which through better survival in the circulation also contributed to the bulk cell progeny of distal tumors. Our findings establish galectin-4 and C1GALT1-mediated glycosylation in a signaling axis that is activated during prostate cancer progression, with implications for therapeutic targeting of advanced metastatic disease. Cancer Res; 76(19); 5756–67. ©2016 AACR.
The Dual Inhibition of RNA Pol I Transcription and PIM Kinase as a New Therapeutic Approach to Treat Advanced Prostate Cancer
Authors: Rebello Richard J. , Kusnadi Eric , Cameron Donald P. , Pearson Helen B. , Lesmana Analia , Devlin Jennifer R. , Drygin Denis , Clark Ashlee K. , Porter Laura , Pedersen John , Sandhu Shahneen , Risbridger Gail P. , Pearson Richard B. , Hannan Ross D. , Furic Luc 
Published: August 2016

AbstractPurpose: The MYC oncogene is frequently overexpressed in prostate cancer. Upregulation of ribosome biogenesis and function is characteristic of MYC-driven tumors. In addition, PIM kinases activate MYC signaling and mRNA translation in prostate cancer and cooperate with MYC to accelerate tumorigenesis. Here, we investigate the efficacy of a single and dual approach targeting ribosome biogenesis and function to treat prostate cancer.Experimental Design:The inhibition of ribosomal RNA (rRNA) synthesis with CX-5461, a potent, selective, and orally bioavailable inhibitor of RNA polymerase I (Pol I) transcription, has been successfully exploited therapeutically but only in models of hematologic malignancy. CX-5461 and CX-6258, a pan-PIM kinase inhibitor, were tested alone and in combination in prostate cancer cell lines, in Hi-MYC- and PTEN-deficient mouse models and in patient-derived xenografts (PDX) of metastatic tissue obtained from a patient with castration-resistant prostate cancer.Results: CX-5461 inhibited anchorage-independent growth and induced cell-cycle arrest in prostate cancer cell lines at nanomolar concentrations. Oral administration of 50 mg/kg CX-5461 induced TP53 expression and activity and reduced proliferation (MKI67) and invasion (loss of ductal actin) in Hi-MYC tumors, but not in PTEN-null (low MYC) tumors. While 100 mg/kg CX-6258 showed limited effect alone, its combination with CX-5461 further suppressed proliferation and dramatically reduced large invasive lesions in both models. This rational combination strategy significantly inhibited proliferation and induced cell death in PDX of prostate cancer.Conclusions: Our results demonstrate preclinical efficacy of targeting the ribosome at multiple levels and provide a new approach for the treatment of prostate cancer. Clin Cancer Res; 22(22); 5539–52. ©2016 AACR.
Therapeutic HPV Cancer Vaccine Targeted to CD40 Elicits Effective CD8+ T-cell Immunity
Authors: Yin Wenjie , Duluc Dorothée , Joo HyeMee , Xue Yaming , Gu Chao , Wang Zhiqing , Wang Lei , Ouedraogo Richard , Oxford Lance , Clark Amelia , Parikh Falguni , Kim-Schulze Seunghee , Thompson-Snipes LuAnn , Lee Sang-Yull , Beauregard Clay , Woo Jung-Hee , Zurawski Sandra , Sikora Andrew G. , Zurawski Gerard , Oh SangKon 
Published: August 2016

Abstract
Human papillomavirus (HPV), particularly HPV16 and HPV18, can cause cancers in diverse anatomical sites, including the anogenital and oropharyngeal (throat) regions. Therefore, development of safe and clinically effective therapeutic vaccines is an important goal. Herein, we show that a recombinant fusion protein of a humanized antibody to CD40 fused to HPV16.E6/7 (αCD40-HPV16.E6/7) can evoke HPV16.E6/7-specific CD8+ and CD4+ T-cell responses in head-and-neck cancer patients in vitro and in human CD40 transgenic (hCD40Tg) mice in vivo. The combination of αCD40-HPV16.E6/7 and poly(I:C) efficiently primed HPV16.E6/7-specific T cells, particularly CD8+ T cells, in hCD40Tg mice. Inclusion of montanide enhanced HPV16.E6/7-specific CD4+, but not CD8+, T-cell responses. Poly(I:C) plus αCD40-HPV16.E6/7 was sufficient to mount both preventative and therapeutic immunity against TC-1 tumors in hCD40Tg mice, significantly increasing the frequency of HPV16-specific CD8+ CTLs in the tumors, but not in peripheral blood. In line with this, tumor volume inversely correlated with the frequency of HPV16.E6/7-specific CD8+ T cells in tumors, but not in blood. These data suggest that CD40-targeting vaccines for HPV-associated malignancies can provide a highly immunogenic platform with a strong likelihood of clinical benefit. Data from this study offer strong support for the development of CD40-targeting vaccines for other cancers in the future. Cancer Immunol Res; 4(10); 823–34. ©2016 AACR.
“We May be Through with the Past …”
Authors: Silverman Jason M. 
Published: August 2016

Recurring themes and traditions within the biblical corpus have attracted much scholarship. This article uses P. T. Anderson’s Magnolia (1999) as a test case for the ways traditions may or may not interact, and what that means for ‘tradition history.’ While the film has many features which strike many viewers as biblical, the author-director denies prior knowledge of these connections. The film is analyzed in terms of structure, Anderson’s claimed sources, the American cultural context of the 1990s, and its biblical resonances. After assessing the import of these observations, the tradition history of Magnolia is compared to the tradition history of the exodus in the Hebrew Bible. Within the framework of attention to media contexts, the article concludes by noting the importance of authority, canon, and multiple lines of transmission. In so doing, our understanding of the transmission of traditions is problematized, and a broader, less text-centric paradigm is called for.
Improving Wind Predictions in the Marine Atmospheric Boundary Layer through Parameter Estimation in a Single-Column Model
Authors: Lee Jared A. , Hacker Joshua P. , Delle Monache Luca , Kosović Branko , Clifton Andrew , Vandenberghe Francois , Rodrigo Javier Sanz 
Published: August 2016

Abstract
A current barrier to greater deployment of offshore wind turbines is the poor quality of numerical weather prediction model wind and turbulence forecasts over open ocean. The bulk of development for atmospheric boundary layer (ABL) parameterization schemes has focused on land, partly because of a scarcity of observations over ocean. The 100-m FINO1 tower in the North Sea is one of the few sources worldwide of atmospheric profile observations from the sea surface to turbine hub height. These observations are crucial to developing a better understanding and modeling of physical processes in the marine ABL.
In this study the WRF single-column model (SCM) is coupled with an ensemble Kalman filter from the Data Assimilation Research Testbed (DART) to create 100-member ensembles at the FINO1 location. The goal of this study is to determine the extent to which model parameter estimation can improve offshore wind forecasts. Combining two datasets that provide lateral forcing for the SCM and two methods for determining , the time-varying sea surface roughness length, four WRF-SCM/DART experiments are conducted during the October–December 2006 period. The two methods for determining are the default Fairall-adjusted Charnock formulation in WRF and use of the parameter estimation techniques to estimate in DART. Using DART to estimate is found to reduce 1-h forecast errors of wind speed over the Charnock–Fairall ensembles by 4%–22%. However, parameter estimation of does not simultaneously reduce turbulent flux forecast errors, indicating limitations of this approach and the need for new marine ABL parameterizations.
Discharge Destination after Head and Neck Surgery
Authors: Cramer John D. , Patel Urjeet A. , Samant Sandeep , Shintani Smith Stephanie 
Published: August 2016

ObjectiveIn recent decades, there has been a reduction in the length of postoperative hospital stay, with a corresponding increase in discharge to postacute care. Discharge to postacute care facilities represents a meaningful patient‐centered outcome; however, little has been published about this outcome after head and neck surgery.Study DesignRetrospective review of national database.SettingAmerican College of Surgeons National Surgical Quality Improvement Program from 2011 to 2013.Subjects and MethodsWe compared the rate of discharge to home versus postacute care facilities in patients admitted after head and neck surgery and used multivariable logistic regression to identify predictors of discharge to postacute care.ResultsThe overall rate of discharge to postacute care facilities after head and neck surgery (n = 15,890) was 15.7% after major surgery (including laryngectomy, composite resection, and free tissue transfer), 4.4% after moderate surgery (including regional tissue transfer, oropharyngeal or oral cavity resection, and neck dissection), and 1.1% after minor head and neck surgery (including endocrine or salivary gland surgery). On multivariable analysis, significant preoperative predictors of discharge to postacute care were advanced age, functional status, major or moderate surgical procedures, tracheostomy, advanced American Society of Anesthesiologists class, low body mass index, and dyspnea.ConclusionOur study indicates that patients undergoing major or moderate head and neck surgery, patients with reduced functional status, and patients with advanced comorbidities are at substantial risk of discharge to postacute care. The possibility of discharge to postacute care should be discussed with high‐risk patients.
Genetic and Environmental Determinants of Otitis Media in an Indigenous Filipino Population
Authors: Santos‐Cortez Regie Lyn P. , Reyes‐Quintos Ma. Rina T. , Tantoco Ma. Leah C. , Abbe Izoduwa , Llanes Erasmo Gonzalo d.V. , Ajami Nadim J. , Hutchinson Diane S. , Petrosino Joseph F. , Padilla Carmencita D. , Villarta Romeo L. , Gloria‐Cruz Teresa Luisa , Chan Abner L. , Cutiongco‐de la Paz Eva Maria , Chiong Charlotte M. , Leal Suzanne M. , Abes Generoso T. 
Published: August 2016

ObjectiveTo identify genetic and environmental risk factors for otitis media in an indigenous Filipino population.Study DesignCross‐sectional study.SettingIndigenous Filipino community.Subjects and MethodsClinical history and information on breastfeeding, tobacco smoke exposure, and swimming were obtained from community members. Heads of households were interviewed for family history and personal beliefs on ear health. Height and weight were measured. Otoscopic findings were described for the presence and character of perforation or discharge. An A2ML1 duplication variant that confers otitis media susceptibility was Sanger sequenced in all DNA samples. Co‐occurrence of middle ear bacteria detected by 16S rRNA gene sequencing was determined according to A2ML1 genotype and social cluster.ResultsThe indigenous Filipino population has a ~50% prevalence of otitis media. Young age was associated with otitis media (4 age strata; P =. 004); however, age was nonsignificant as a bistratal or continuous variable. There was no association between otitis media and sex, body mass index, breastfeeding, tobacco exposure, or deep swimming. In multivariate analyses, A2ML1 genotype is the strongest predictor of otitis media, with an odds ratio of 3.7 (95% confidence interval: 1.3‐10.8; P =. 005). When otitis media diagnoses were plotted across ages, otitis media was observed within the first year of life, and chronic otitis media persisted up to adulthood, particularly in A2ML1‐variant carriers.ConclusionAmong indigenous Filipinos, A2ML1 genotype is the primary risk factor for otitis media and main determinant of disease progression, although age, the middle ear microbiome, and social clusters might modulate the effect of the A2ML1 genotype.
Laryngeal Verrucous Carcinoma: A Systematic Review
Authors: Echanique Kristen A. , Desai Stuti V. , Marchiano Emily , Spinazzi Eleonora F. , Strojan Primož , Baredes Soly , Eloy Jean Anderson 
Published: August 2016

ObjectiveLaryngeal verrucous carcinoma (LVC) is a rare, locally invasive neoplasm comprising 1% to 3.4% of laryngeal carcinomas. Management strategies are a topic of ongoing conversation, and no definitive treatment protocol based on T stage and presentation exists. This review examines characteristics, treatment modalities, and patient outcomes of LVC.Data SourcesPubMed, MEDLINE, EMBASE, and Web of Science.MethodsDatabases were searched through October 29, 2015, for literature detailing individual patient cases of LVC. Variables analyzed included patient demographics, tumor characteristics, tumor size, treatment, and outcomes.ResultsThirty‐seven articles with 369 cases were included. LVC was found more commonly in males (13.8:1), at an average age of 58.7 years, and located in the glottis (74.0%). Most patients had local disease at presentation (94.9%). The most common presenting symptom was hoarseness (92.3%). The most common primary treatment was surgery alone (72.3%), with local excision as the most common technique (56.8%). In patients with data available on both surgical modality and T stage, most patients who presented as T1 and were managed surgically underwent local excision (79.2%). Surgical treatment alone led to high rates of disease‐free survival at follow‐up (86.8%). A large number of patients presenting with T1 disease were disease free at follow‐up (88.6%). Overall survival was 80.3%.ConclusionLVC is most often managed surgically. The extent of surgical resection may be guided by T stage, with smaller tumors resected via local excision and larger tumors via partial or total laryngectomy. Regardless of T stage or therapy, LVC has a good posttreatment prognosis.
Predominant expression of Alzheimer’s disease-associated BIN1 in mature oligodendrocytes and localization to white matter tracts
Authors: De Rossi Pierre , Buggia-Prévot Virginie , Clayton Benjamin L. L. , Vasquez Jared B. , van Sanford Carson , Andrew Robert J. , Lesnick Ruben , Botté Alexandra , Deyts Carole , Salem Someya , Rao Eshaan , Rice Richard C. , Parent Angèle , Kar Satyabrata , Popko Brian , Pytel Peter , Estus Steven , Thinakaran Gopal 
Published: August 2016

Abstract
Background
Genome-wide association studies have identified BIN1 within the second most significant susceptibility locus in late-onset Alzheimer’s disease (AD). BIN1 undergoes complex alternative splicing to generate multiple isoforms with diverse functions in multiple cellular processes including endocytosis and membrane remodeling. An increase in BIN1 expression in AD and an interaction between BIN1 and Tau have been reported. However, disparate descriptions of BIN1 expression and localization in the brain previously reported in the literature and the lack of clarity on brain BIN1 isoforms present formidable challenges to our understanding of how genetic variants in BIN1 increase the risk for AD.

Methods
In this study, we analyzed BIN1 mRNA and protein levels in human brain samples from individuals with or without AD. In addition, we characterized the BIN1 expression and isoform diversity in human and rodent tissue by immunohistochemistry and immunoblotting using a panel of BIN1 antibodies.

Results
Here, we report on BIN1 isoform diversity in the human brain and document alterations in the levels of select BIN1 isoforms in individuals with AD. In addition, we report striking BIN1 localization to white matter tracts in rodent and the human brain, and document that the large majority of BIN1 is expressed in mature oligodendrocytes whereas neuronal BIN1 represents a minor fraction. This predominant non-neuronal BIN1 localization contrasts with the strict neuronal expression and presynaptic localization of the BIN1 paralog, Amphiphysin 1. We also observe upregulation of BIN1 at the onset of postnatal myelination in the brain and during differentiation of cultured oligodendrocytes. Finally, we document that the loss of BIN1 significantly correlates with the extent of demyelination in multiple sclerosis lesions.

Conclusion
Our study provides new insights into the brain distribution and cellular expression of an important risk factor associated with late-onset AD. We propose that efforts to define how genetic variants in BIN1 elevate the risk for AD would behoove to consider BIN1 function in the context of its main expression in mature oligodendrocytes and the potential for a role of BIN1 in the membrane remodeling that accompanies the process of myelination.
Repair of traumatized mammalian hair cells via sea anemone repair proteins
Authors: Tang Pei-Ciao , Smith Karen Müller , Watson Glen M. 
Published: August 2016

ABSTRACT
Mammalian hair cells possess only a limited ability to repair damage after trauma. In contrast, sea anemones show a marked capability to repair damaged hair bundles by means of secreted repair proteins (RPs). Previously, it was found that recovery of traumatized hair cells in blind cavefish was enhanced by anemone-derived RPs; therefore, the ability of anemone RPs to assist recovery of damaged hair cells in mammals was tested here. After a 1 h incubation in RP-enriched culture media, uptake of FM1-43 by experimentally traumatized murine cochlear hair cells was restored to levels comparable to those exhibited by healthy controls. In addition, RP-treated explants had significantly more normally structured hair bundles than time-matched traumatized control explants. Collectively, these results indicate that anemone-derived RPs assist in restoring normal function and structure of experimentally traumatized hair cells of the mouse cochlea.