bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_0020397 regulates colorectal cancer cell viability, apoptosis and invasion by promoting the expression of the miR‐138 targets TERT and PD‐L1
Authors: Zhang Xiu‐li , Xu Ling‐ling , Wang Fang 
Published: July 2017

AbstractColorectal cancer (CRC) is a common human gastrointestinal cancer, and recent studies indicate that circular RNA (circRNA) may regulate cancer development. In this study, we assess the role of circRNA specifically in colorectal cancer. Our quantitative PCR assays demonstrate an upregulation of the circRNA has_circ_0020397 and a downregulation of miR‐138 in CRC cells, as well as a negative correlation between these two. Using a dual‐luciferase reporter assay, we show evidence of miR‐138‐binding sites on hsa_circ_0020397, and that overexpression of hsa_circ_0020397 could inhibit the downregulation of luciferase activity by miR‐138. Although hsa_circ_0020397 did not influence miR‐138 expression per se, has_circ_0020397 did inhibit miR‐138 activity, as examined via the expression of miR‐138 targets telomerase reverse transcriptase (TERT) and programmed death‐ligand 1 (PD‐L1). Control treatments with plasmids overexpressing linear hsa_circ_0020397 did not have these effects. Hsa_circ_0020397 promoted cell viability and invasion of CRC cells and inhibited their apoptosis, whereas miR‐138 had the opposite effect. Nevertheless, hsa_circ_0020397 antagonized miR‐138 suppression of cell growth. When TERT or PD‐L1 expression was suppressed with siRNAs, the above functions of hsa_circ_0020397 were attenuated, suggesting that hsa_circ_0020397 can regulate CRC cell viability, apoptosis and invasion by promoting the expression of miR‐138 target genes. These findings support the role of circRNA in CRC pathogenesis.
Individualizing dual antiplatelet therapy duration: Prediction tools, genomics, and clinical judgment
Authors: Chatterjee Arka , Hillegass William B. 
Published: July 2017

Key Points

Prolonged dual antiplatelet therapy (DAPT) after percutaneous coronary intervention (PCI) on average reduces the risk of subsequent myocardial infarction (MI) but increases major bleeds.
Individualizing duration of DAPT based on the DAPT trial's net benefit prediction tool would likely optimize outcome beyond population average recommendations. Individualizing agent selection and duration of therapy based on genomic data may further improve outcomes.
Clinical judgment remains the most important tool to tailor DAPT duration based on a large array of additional relevant factors not captured by predition rules or genomics.
Paravalvular leak versus need for permanent pacemaker after TAVR: Sailing between Scylla and Charybdis
Authors: Cilingiroglu Mehmet , Marmagkiolis Konstantinos 
Published: July 2017

Key Points

Absence or minimization of PVL after TAVR and its potential association with higher rates of PPM placement has been the Achilles’ heel of TAVR procedures.
Pre‐existing RBBB and depth of implantation are independent predictors of PPM need after Lotus valve implantation.
Optimization of device design, increased operator experience, and focus on accurate device placement seem to result in decreased need for PPM after Lotus valve implantation.
Sleep deprivation in interventional cardiology: Implications for patient care and physician‐health
Authors: Sandoval Yader , Lobo Angie S. , Somers Virend K. , Rosenfield Kenneth , Bradley Steven M. , Sorajja Paul , Tajti Peter , Brilakis Emmanouil S. 
Published: July 2017

AbstractThe burden and impact of sleep deprivation on both patient care and on the health of interventional cardiologists is not well understood. Due to the nature of emergent procedures occurring in the cardiac catheterization laboratory, interventionalists are prone to suffer from acute and/or chronic sleep deprivation. Sleep deprivation has been associated with numerous adverse effects, such as impaired performance, cognitive deficits, reduced psychomotor vigilance, and workplace errors and injuries, among many others. Although sleep deprivation has been linked to more errors in trainees, there is paucity of data addressing outcomes in interventional cardiology. The purpose of this overview is to explore the possible impact of sleep deprivation on interventional cardiology in relation to patient care and physician health, and examine potential approaches to this issue.
Managing bisphosphonate‐related osteonecrosis of the jaws with xenografts: a case report
Authors: de Oliveira Ruellas Aluísio Martins , Peruzzo Daiane Cristina , Napimoga Marcelo Henrique 
Published: July 2017

Key Clinical MessageIt is possible to manage large bone destruction induced by BRONJ using xenograft grafting to promote a bone formation.
Repeated successful use of eltrombopag in chronic primary immune thrombocytopenia: description of an intriguing case
Authors: Santoro Cristina , Volpicelli Paola , Baldacci Erminia , Ferrara Grazia , Di Rocco Alice , Ferretti Antonietta , Porrazzo Marika , Mazzucconi Maria Gabriella 
Published: July 2017

Key Clinical MessageThrombopoietin receptor agonists (TPO‐RAs) are used as effective alternative treatments in ITP patients unresponsive to first‐/second‐line therapies. TPO‐RAs can also be used to normalize platelet count to safely perform invasive procedures and chemotherapy, in case of malignancies. In few responsive patients, TPO‐RAs can be suspended maintaining a sustained response.
Theoretical Explanation of the Photogenerated Carrier Separation at the Surface Junction
Authors: Song Weiyu , Ma Sicong , Wang Lu , Liu Jian , Zhao Zhen 
Published: July 2017

AbstractFor photocatalytic reactions, the fabrication of surface junction has been proven to be an effective method to increase the photogenerated carrier (excited electrons and photogenerated holes) separation efficiency. However, the carrier separation mechanism has not been studied in detail. In this work, density functional theory has been employed to study the photogenerated carrier‐separation mechanism based on an interfacial model consisting of TiO2 (1 0 1) and (0 0 1) facets. Both excited electrons and photogenerated holes preferentially locate on the (0 0 1) facets rather than the (1 0 1) facets. However, the high conduction‐band energy level of the interface inhibits the electron interfacial migration. In contrast to the exited‐electrons migration process, the suitable valence‐band energy of interface permits the hole interfacial migration from the (1 0 1) to the (0 0 1) facets. The electrons easily recombine with the holes on the (0 0 1) facets owing to the holes overflow effect. Excited electrons and holes would fill the (1 0 1) facets and (0 0 1) facets, respectively. Based on such interfacial energy band properties, we propose a new photocatalytic material design concept, named “selective migration” structure, which can promote the photogenerated carrier separation and offer more strongly reductive electrons for the photoreduction reaction or more strongly oxidative holes for the photooxidation reaction. These results are expected to provide a deeper understanding of the surface junction and a guide for designing new photocatalysts.
Functionalities tuned enantioselectivity of phenylcarbamate cyclodextrin clicked chiral stationary phases in HPLC
Authors: Tang Jian , Lin Yuzhou , Yang Bo , Zhou Jie , Tang Weihua 
Published: July 2017

AbstractThe mixed chloro‐ and methyl‐ functionalities can greatly modulate the enantioselectivities of phenylcarbamate cyclodextrin (CD) clicked chiral stationary phases (CSPs). A comparison study is herein reported for per(4‐chloro‐3‐methyl)phenylcarbamate and per(2‐chloro‐5‐methyl)phenylcarbamate β‐CD clicked CSPs (i.e., CCC4M3‐CSP and CCC2M5‐CSP). The enantioselectivity dependence on column temperature was studied in both normal‐phase and reversed‐phase mode high performance liquid chromatography (HPLC). The thermodynamic study revealed that the stronger intermolecular interactions can be formed between CCC4M3‐CSP and chiral solutes to drive the chiral separation. The higher enantioselectivities of CCC4M3‐CSP were further demonstrated with the enantioseparation of 17 model racemates in HPLC.
Early evidence of irrigation technology in the North China Plain: Geoarchaeological investigations at the Anshang Site, Neihuang County, Henan Province, China
Authors: Storozum Michael , Liu Haiwang , Qin Zhen , Ming Kongde , Fu Kui , Wang Hui , Kidder Tristram 
Published: July 2017

ABSTRACTThe development of irrigation is a politically important technology that enabled agricultural societies to intensify agricultural production. In the North China Plain, the historical record suggests a long tradition of irrigation, but archaeologists have found scant evidence of these technologies outside of urban areas. In 2012, 2015, and 2016, our excavations at the Anshang site, Neihuang County, Henan Province, China, revealed several archaeological features that we interpret as evidence of Late Shang dynasty (ca. 1200–1046 B.C.) canal construction in a nonurban context. By using a range of geological methods, including particle size, loss on ignition, magnetic susceptibility, and micromorphology, we suggest that these ancient canals and the subsequent construction phases found at Anshang not only influenced the development of local geomorphic conditions, but also were part of early dynastic attempts to restructure the natural landscape to facilitate agricultural productivity. These irrigation canals found at Anshang may be representative of broader trends of human‐environmental interactions in the North China Plain.
Nodular lymphocyte predominant Hodgkin's lymphoma in daily practice: A multicenter experience
Authors: Gemici Aliihsan , Aydogdu Ismet , Terzi Hatice , Sencan Mehmet , Aslan Alma , Kaya Ali Hakan , Dal Mehmet Sinan , Akay Meltem Olga , Dogu Mehmet Hilmi , Ayyildiz Orhan , Sahin Fahri , Cagliyan Gulsum Akgun , Yilmaz Mehmet , Gokgoz Zafer , Bilen Yusuf , Demir Cengiz , Sevindik Omur Gokmen , Korkmaz Serdal , Eser Bulent , Altuntas Fevzi ,   ThREG, Turkish hematology Research and Education Group
Published: July 2017

AbstractNodular lymphocyte predominant Hodgkin's lymphoma (NLPHL) is a rare subtype of Hodgkin's lymphoma. In this study, we aimed to investigate the clinical features and therapeutic outcomes of patients with NLPHL who were diagnosed at different institutes in Turkey.We retrospectively reviewed the records of the patients diagnosed with NLPHL. Adult patients who were diagnosed after 2005 with histological confirmation were selected for the study. Forty‐three patients were included in the study. Median age of patients was 37.5 years (18‐70) at the time of diagnosis. About 60.5% patients were diagnosed as stage I and II NLPHL, and remaining 39.5% had stage III and IV disease. Median follow‐up was 46 months. During follow‐up, none of the patients died. Seven patients relapsed or progressed after initial therapy at a median of 12 months. Five of 7 relapsed/refractory patients (71.4%) were salvaged with chemotherapy only (DHAP, ICE), and the remaining 2 (28.6%) were salvaged with chemoimmunotherapy. All of relapsed/refractory patients were able to achieve complete remission after salvage therapy. Lactate dehydrogenase levels were significantly higher in patients with progressive disease compared with nonprogressive disease. Our study showed an excellent outcome with all patients alive at last contact with a median follow up of 46 months despite a wide range of different therapeutic approaches. All relapsed and refractory patients were successfully salvaged despite a low frequency of patients received immunotherapy in conjunction with chemotherapy. Our results suggest that immunotherapy may be reserved for further relapses.
Bone healing in an aged murine fracture model is characterized by sustained callus inflammation and decreased cell proliferation
Authors: Hebb John H. , Ashley Jason W. , McDaniel Lee , Lopas Luke A. , Tobias John , Hankenson Kurt D. , Ahn Jaimo 
Published: July 2017

ABSTRACTGeriatric fractures take longer to heal and heal with more complications than those of younger patients; however, the mechanistic basis for this difference in healing is not well understood. To improve this understanding, we investigated cell and molecular differences in fracture healing between 5‐month‐old (young adult) and 25‐month‐old (geriatric) mice healing utilizing high‐throughput analysis of gene expression. Mice underwent bilateral tibial fractures and fracture calluses were harvested at 5, 10, and 20 days post‐fracture (DPF) for analysis. Global gene expression analysis was performed using Affymetrix MoGene 1.0 ST microarrays. After normalization, data were compared using ANOVA and evaluated using Principal Component Analysis (PCA), CTen, heatmap, and Incromaps analysis. PCA and cross‐sectional heatmap analysis demonstrated that DPF followed by age had pronounced effects on changes in gene expression. Both un‐fractured and 20 DPF aged mice showed increased expression of immune‐associated genes (CXCL8, CCL8, and CCL5) and at 10 DPF, aged mice showed increased expression of matrix‐associated genes, (Matn1, Ucma, Scube1, Col9a1, and Col9a3). Cten analysis suggested an enrichment of CD8+ cells and macrophages in old mice relative to young adult mice and, conversely, a greater prevalence of mast cells in young adult mice relative to old. Finally, consistent with the PCA data, the classic bone healing pathways of BMP, Indian Hedgehog, Notch and Wnt clustered according to the time post‐fracture first and age second. Clinical Significance: Greater understanding of age‐dependent molecular changes with healing will help form a mechanistic basis for therapies to improve patient outcomes. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:149–158, 2018.
Identification and quantification of phenolic compounds in edible wild leafy vegetables by UHPLC/Orbitrap‐MS
Authors: Giambanelli Elisa , Filippo D'Antuono L , Romero‐González Roberto , Garrido Frenich Antonia 
Published: July 2017

AbstractBACKGROUNDA recent interest in edible wild leafy vegetables has been documented. Consumers often associate these species with health promotion. In this study, several wild species of the Asteraceae family andKnautia integrifolia(Dipsacaceae) were locally documented for their use in traditional cuisine and sampled from the wild.RESULTSPhenolic compounds were identified and quantified by ultra‐high‐performance liquid chromatography coupled to Orbitrap high‐resolution mass spectrometry. Hydroxycinnamic acids ranging from 1388 to 53 076 mg kg−1dry weight (DW) were the most abundant compounds in all species (69–98% of the total phenolic content) exceptTragopogon pratensis. Thirty compounds were identified as flavonoids, mostly as glycosidic forms of luteolin, apigenin, kaempferol and quercetin. The sum of flavonoids ranged between 212 and 12 598 mg kg−1DW; they represented 65% of the total phenolic content forT. pratensis. Three anthocyanins were detected, representing in most cases less than 1% of the total phenolic content (3–627 mg kg−1DW). Higher anthocyanin contents were observed forCichoriumtypes.CONCLUSIONDifferent phenolic profiles were observed between species, especially considering the class of flavonoids. Individual species may be of some interest for their content of specific minor flavonoids. © 2017 Society of Chemical Industry
In vitro renin–angiotensin system inhibition and in vivo antihypertensive activity of peptide fractions from lima bean (Phaseolus lunatus L.)
Authors: Ciau‐Solís Norma A , Acevedo‐Fernández Juan J , Betancur‐Ancona David 
Published: July 2017

AbstractBACKGROUNDThe renin–angiotensin system is key in the physiopathology of arterial hypertension because it converts angiotensin I, via angiotensin I‐converting enzyme (ACE), into angiotensin II. In vitro analyses were done of the ACE‐inhibitory and renin‐inhibitory activities of peptide fractions isolated by enzymatic hydrolysis of lima bean (Phaseolus lunatus) protein. Antihypertensive activity was confirmed in vivo using a rat model.RESULTSLima bean protein was hydrolyzed with one of two sequential enzymatic systems (pepsin–pancreatin or Alcalase®–Flavourzyme®). Ultrafiltration of the hydrolysates produced fractions of different molecular weights. The >3 kDa fraction of the pepsin–pancreatin hydrolysate had the highest ACE‐inhibitory activity (60.15%, IC50: 172.62 µg mL−1), while the >3 KDa fraction of the Alcalase®–Flavourzyme® hydrolysate had the highest in vitro renin‐inhibitory activity. A weak correlation (r = 0.44) was found between ACE‐inhibitory and renin‐inhibitory activities. When tested in vivo, the latter fraction lowered systolic blood pressure by 64% and diastolic blood pressure by 51%.CONCLUSIONPeptide fractions from lima bean Phaseolus lunatus protein hydrolysates exhibit both in vitro and in vivo antihypertensive activity. Bioactive peptides from lima bean have potential applications as ingredients in functional foods. © 2017 Society of Chemical Industry
Utility of 360° Real‐time Endoanal Sonography for Evaluation of Perianal Fistulas
Authors: Sun Yan , Cui Li‐Gang , Liu Ji‐Bin , Wang Jin‐Rui , Ping He , Chen Zhao‐Wen 
Published: July 2017

ObjectivesTo assess the diagnostic accuracy and performance of 360° endoanal sonography for preoperative evaluation of perianal fistulas using surgical results as a reference standard.MethodsBetween January 2012 and May 2016, 122 patients with suspicious anal fistulas and perianal abnormalities had real‐time endoanal sonographic examinations using a 360° cross‐sectional endoanal transducer (5–13 MHz) for preoperative evaluation of the disease. All patients underwent surgical interventions. The sonographic findings, including the fistulas and other inflammatory lesions, were correlated with surgical results. The types of fistulas on endoanal sonography were compared with the surgical classification of Parks et al (Br J Surg 1976; 63:1–2), and the internal opening of the fistula on endoanal sonography was confirmed by surgical results as a reference standard.ResultsThe 122 patients studied included 111 male and 11 female patients. Endoanal sonography was able to show and track hypoechoic lesions, their locations, and internal openings of the fistulas. Compared with surgical results, endoanal sonography had sensitivity of 92.2%, specificity of 100%, and accuracy of 93.4% for the diagnosis of perianal fistulas. Also, endoanal sonography had accuracy of 87.4% for determining fistula types based on the Parks classification and 94.6% for identifying internal openings of the fistulas.ConclusionsEndoanal sonography is an accurate and noninvasive imaging modality for evaluation of fistulas. It is a very useful tool for preoperative management and surgical planning by providing precise and detailed information on fistulas.
Response of synthetic diamond detectors in proton, carbon, and oxygen ion beams
Authors: Rossomme Séverine , Marinelli Marco , Verona‐Rinati Gianluca , Romano Francesco , Cirrone Pablo Antonio Giuseppe , Kacperek Andrzej , Vynckier Stefaan , Palmans Hugo 
Published: July 2017

PurposeIn this work, the LET‐dependence of the response of synthetic diamond detectors is investigated in different particle beams.MethodMeasurements were performed in three nonmodulated particle beams (proton, carbon, and oxygen). The response of five synthetic diamond detectors was compared to the response of a Markus or an Advanced Markus ionization chamber. The synthetic diamond detectors were used with their axis parallel to the beam axis and without any bias voltage. A high bias voltage was applied to the ionization chambers, to minimize ion recombination, for which no correction is applied (+300 V and +400 V were applied to the Markus and Advanced Markus ionization chambers respectively).ResultsThe ratio between the normalized response of the synthetic diamond detectors and the normalized response of the ionization chamber shows an under‐response of the synthetic diamond detectors in carbon and oxygen ion beams. No under‐response of the synthetic diamond detectors is observed in protons. For each beam, combining results obtained for the five synthetic diamond detectors and considering the uncertainties, a linear fit of the ratio between the normalized response of the synthetic diamond detectors and the normalized response of the ionization chamber is determined. The response of the synthetic diamond detectors can be described as a function of LET as (−6.22E‐4 ± 3.17E‐3) • LET + (0.99 ± 0.01) in proton beam, (−2.51E‐4 ± 1.18E‐4) • LET + (1.01 ± 0.01) in carbon ion beam and (−2.77E‐4 ± 0.56E‐4) • LET + (1.03 ± 0.01) in oxygen ion beam. Combining results obtained in carbon and oxygen ion beams, a LET dependence of about 0.026% (±0.013%) per keV/μm is estimated.ConclusionsDue to the high LET value, a LET dependence of the response of the synthetic diamond detector was observed in the case of carbon and oxygen beams. The effect was found to be negligible in proton beams, due to the low LET value. The under‐response of the synthetic diamond detector may result from the recombination of electron/hole in the thin synthetic diamond layer, due to the high LET‐values. More investigations are required to confirm this assumption.
Surface‐attached polymer networks through carbene intermediates generated from α‐diazo esters
Authors: Osterwinter Gregor J. , Navarro‐Crespo Rodrigo , Prucker Oswald , Henze Michael , Rühe Jürgen 
Published: July 2017

ABSTRACTWe report on the generation of surface attached polymer networks through C,H insertion reactions based on carbene intermediates. To this copolymers based on N,N‐dimethyl acrylamide, which contain α‐diazo ester groups, are generated and coated onto a solid substrate covered with a self‐assembled monolayer of a silane. After deposition, films having thicknesses of approximately 120 nm are irradiated with UV light having a wavelength of 254 nm or 360 nm or heated to temperatures between 80 and 180 °C. During the light or heat‐induced activation process, carbenes are formed, which react via C—H insertion with any neighboring polymer chain and/or with the self‐assembled monolayer on the substrate, thus forming surface‐attached polymer networks. To follow the kinetics of the crosslinking process, the conditions are adjusted, so that they range between 5 and 10 min for the UV‐crosslinking and 5 to 15 min for the heat‐induced crosslinking. UV‐patterning of the surface‐attached polymer networks through chrome contact masks allows generation of microstructures in the micrometer range. We investigate the layer formation process and describe the formation of microstructured surfaces based on these surface‐attached polymer networks. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 3276–3285
UCSF ChimeraX: Meeting modern challenges in visualization and analysis
Authors: Goddard Thomas D. , Huang Conrad C. , Meng Elaine C. , Pettersen Eric F. , Couch Gregory S. , Morris John H. , Ferrin Thomas E. 
Published: July 2017

AbstractUCSF ChimeraX is next‐generation software for the visualization and analysis of molecular structures, density maps, 3D microscopy, and associated data. It addresses challenges in the size, scope, and disparate types of data attendant with cutting‐edge experimental methods, while providing advanced options for high‐quality rendering (interactive ambient occlusion, reliable molecular surface calculations, etc.) and professional approaches to software design and distribution. This article highlights some specific advances in the areas of visualization and usability, performance, and extensibility. ChimeraX is free for noncommercial use and is available from http://www.rbvi.ucsf.edu/chimerax/ for Windows, Mac, and Linux.