bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex differences in sucrose-induced locomotor sensitization and cross-sensitization with the D2/D3 agonist quinpirole in rats
Authors: Matic Vangel , Huynh Matthew , Bishnoi Indra R. , Kavaliers Martin , Ossenkopp Klaus-Peter 
Published: September 2024

Abstract

Background: Female-biased sex differences are known to exist in the prevalence of eating-related disorders and the experience of food cravings. Similarly, in rodents, females display a greater preference and motivation for the highly palatable sweet food, sucrose, than do males. Locomotor sensitization, the increase in locomotor activity that occurs with repeated administrations of drugs of abuse, is thought to occur through drug-induced sensitization of the mesolimbic dopamine pathway and reflect enhancements in drug craving. Pre-exposure to sucrose has been shown to enhance locomotor sensitization induced by dopaminergic agonists, however, it is unknown if sex differences exist in this effect.
Methods: Female (n = 16) and male (n = 16) Long-Evans rats received 30 min daily access to sucrose (0.3 M) or water for nine consecutive days followed by daily administration of the D2/D3 agonist, quinpirole (0.5 mg/kg), for nine consecutive days. On the first, fifth, and ninth day of the sucrose and quinpirole phases, rats were placed in automated activity monitors for 30 min after fluid access and drug administration, respectively, to assess locomotor activity. Mixed model analyses of variance were used to evaluate differences in locomotor activity between Female + Sucrose, Female + Water, Male + Sucrose, and Male + Water treated rats.
Results: Female + Sucrose rats showed significantly greater weight-adjusted fluid consumption of sucrose than did Male + Sucrose rats. Female + Sucrose rats exhibited a significant increase in locomotor activity across the sucrose phase and significantly greater locomotor activity relative to other groups. In the quinpirole phase, both groups pre-exposed to sucrose (Female + Sucrose, Male + Sucrose) showed greater activity than their same-sex control pre-exposed to water (Female + Water, Male + Water). Further, both female groups generally displayed greater activity than both male groups in the quinpirole phase.
Conclusions: A female-biased sex difference was identified in the effect of locomotor sensitization induced by sucrose (0.3 M), but not for sucrose pre-exposure on quinpirole sensitization (0.5 mg/kg). These results suggest that differences may exist between sexes in the mesolimbic response to sucrose, perhaps underlying sex differences in food cravings and eating-related disorder prevalence.
Nursing Students' Perception and Associated Factors Towards the Nursing Profession at Selected Health Science Colleges in Addis Ababa, Ethiopia: A Cross-sectional study.
Authors: Hassen Rahmet , Habte Teshome , Dugassa Boka 
Published: September 2024

Abstract

Background: Understanding nursing students' perceptions of their profession is crucial issue for improving educational programs, enhancing support systems, and ultimately preparing competent and compassionate health care professionals.
Objective: This study aimed to assess nursing students' perceptions and the associated factors of the nursing profession at selected health science colleges in Addis Ababa, Ethiopia.
Methods: A cross-sectional study was conducted from Feb 19- Mar 19, 2024, among nursing students at four health science colleges. Data was collected by using structured interview questionnaires after preparation and validated by using expert review from randomly selected undergraduate Nursing students enrolled in the four health science colleges of Addis Ababa. And organized students' socio-demographic characteristics, perceptions towards nursing, and associated factors and presented Descriptive statistics, Bivariate and multiple logistic regression analysis were computed using SPSS version 26.
Results: A total of 267 nursing students participated in this study yielding a 100% response rate. The majority (57.3%) of the students had a positive perception of the nursing profession. Factors significantly associated with positive perception included female gender, higher family income, and having a close family member who is a nurse. Factors associated with negative perception included poor clinical supervision and inadequate resources in clinical settings. From this, 82% (n=219) of the study participants were female nurses. Being female (AOR = 5.77; 95% CI: 2.47 – 13.44), competitive salary like other professions (AOR = 3.48; 95% CI: 1.81 – 6.66), having reasons for enrolling in the nursing profession (AOR= 4.39; 95% CI:2.37– 8.13) were significantly associated with the perception of nurses towards their profession.
Conclusion: Nursing students generally had a positive perception of the nursing profession. However, factors related to the clinical learning environment heavy workload, lack of professional autonomy, and poor remuneration was associated with a negative perception. Addressing these factors through improved clinical placements, and awareness creation towards the nursing profession should be implemented before and after students join the nursing school.
MRI-ASL-based CBF changesduring the interictal period after anti-CGRP therapy in migraine patients
Authors: Shimoda Masami , Hoshikawa Kaori , Oda Shinri , Imai Masaaki , Aoki Rie , Shinohara Chiaki 
Published: September 2024

Abstract

Objective: Migraine and insomnia often accompany migraine are not steady-state conditions because cerebral blood flow (CBF) autoregulation and the glymphatic system are impaired. Anti-endogenous anti-calcitonin gene-related peptide (CGRP) therapy may thus reduce the interictal CBF.
Methods: Interictal magnetic resonance arterial spin labeling (ASL) was performed on 73 migraineurs before and after anti-CGRP therapy, including antibody switching, to investigate changes in CBF and predictors of treatment response.
Results: Multivariate analysis revealed that the significant positive neuroradiological predictors were no migraine with cortical hyperperfusion (CHP) findings despite insomnia and no insomnia with white matter hyperintensities. Patients in this study were divided into four clinical groups according to CBF changes after anti-CGRP therapy: Group A, CHP findings before treatment and decreased CBF after treatment (27 patients); Group B, no CHP before treatment and decreased CBF after treatment (19 patients); Group C, no CHP before treatment and increased CBF after treatment (18 patients); and Group D, no change in CBF before or after treatment. Changes in CBF after therapy were appropriate in Groups A and C; in particular, Group C had the highest percentage of ³ 50% of the responders, reaching 94%. Approximately 90% of patients in Group B showed an inappropriate reduction in CBF below normal after treatment. All but 1 patient in Group D did not respond to treatment.
Conclusion: Interictal CBF measurements by ASL before and after anti-CGRP therapy are clinically useful biomarkers for predicting and evaluating treatment efficacy.
The Anatoly Silantiev Memorial Readings, dedicated to the 150-th anniversary of the birth: Game management in Russia. History and modernity
Authors: Селиховкин А.В. , Масайтис В.В. , Николаева М.А. , Варенцова Е.Ю. 
Published: September 2024

Конференция проводится кафедрой защиты леса, древесиноведения и охотоведения при поддержке Санкт-Петербургского государственного лесотехнического университета имени С.М. Кирова.
Comparative analysis of Health-Related Quality of Life, anxiety and depression in patients with alopecia areata, atopic dermatitis and with association of these diseases
Authors: Tereshchenko G. P. , Gadzhigoroeva A. G. , Romanova Yu. Yu. , Potekaev N. N. 
Published: September 2024

Introduction. Alopecia areata (AA) and atopic dermatitis (AtD) are associated with significant psychosocial burden, emphasizing the importance of quality of life (QoL) assessment due to potential psychological distress and treatment hindrance.Aim. To conduct a comparative analysis of QoL, anxiety and depression in patients with AA, AtD and their combination, according to diseases severity.Materials and methods. The study included 91 patients of both sexes (18–52 years old). All patients were divided into 3 groups: group 1 – 25% patients with AA, group 2 – 28% with AtD, group 3 – 47% patients with both diseases; the groups were subdivided by dermatoses severity. The DLQI, Skindex-29 and HADS questionnaires were used. Mann-Whitney U-test was conducted to compare the mean values of quantitative data (М ± n) (p < 0.05).Results. Group 1 had moderately decreased QoL according to DLQI (6.5 ± 0.67); total Skindex-29 score was 23.55 ± 2.46 (low impact) with the highest value in the emotion domain. In group 2 DLQI score was 12.5 ± 1.51, total Skindex-29 score was48.41 ± 3.76; in group 3 DLQI was 11.5 ± 0.58, Skindex-29 – 50.46 ± 2.14. These values corresponded to very negative impact on QoL, increasing with diseases severity. There was a reliable difference in DLQI and Skindex-29 values between groups 1 and 2 and groups 1 and 3. Mean HADS scores in all groups were generally comparable and correlated with AtD and AA severity.Conclusion. Compared to AA, AtD has a greater impact on QoL, involving all areas of social health; AA predominantly affects emotional sphere. The presence of both diseases worsens QoL as well as AtD alone, especially in aspects “emotions” and “functions”.
New topical combinations in acne therapy
Authors: Katkhanova О. A. 
Published: September 2024

Introduction. Therapeutic treatment modalities for acne involve both topical and oral medications. Fixed-dose combinations that affect all components of the pathogenesis of acne vulgaris are given preference among topical medications.Aim. To evaluate the efficacy and tolerability of Metrogyl® a topical drug, containing adapalene and metronidazole, which is used as topical therapy in patients with mild to moderate acne.Materials and methods. A prospective, open-label, randomized, single-centre comparative study was conducted in 2022. A total of 60 patients were subjected to the Mir-O-Med clinic-based observation (Krasnodar): 19 men and 41 women aged 18 to 45 years with mild to moderate acne. Group 1 received a fixed-dose combination of adapalene 1.0 mg and metronidazole10.0 mg (Metrogyl® A); Group 2 – a fixed-dose combination of adapalene 0.1% and clindomycin phosphate 1%; Group 3 – a fixed-dose combination of adapalene 0.1% and benzoyl peroxide 2.5% gel; Group 4 – adapalene 0.1%. Group 5, a separately created group, included patients from Group 1, who continued therapy for another 3 months.Results. After therapy, 6 (40%) patients from Group 1 archived clinical remission, and 9 (60%) patients found significant improvement. No deterioration in patients’ condition or absence of clinical effects was observed during therapy. The combination of adapalene and metronidazole (Metrogyl® A) to treat mild to moderate acne is comparable in terms of efficacy to the combination of adapalene and benzoyl peroxide, 10% more efficient than topical adapalene therapy and 30% more efficient than topical antibiotics combination.Conclusion. The obtained results showed high therapeutic efficacy, good tolerability and safety of Metrogyl® A, which allows to use it as monotherapy and as part of combination with other agents in patients with mild to moderate acne.
ÍNDICE DE ANOMALIA DE CHUVA PARA DIFERENTES MESORREGIÕES DO ESTADO DE PERNAMBUCO
Authors: Alves Jandelson de Oliveira , Pereira Poliana de Caldas , De Queiroz Maria Gabriela , Da Silva Thieres George Freire , Ferreira Jadna Mylena da Silva , Araújo Júnior George do Nascimento 
Published: September 2024

Uma das formas de estudar a precipitação pluviométrica é através de Índices de Anomalia de Chuva (IAC), que ajudam a monitorar anos de seca e chuva excessiva, permitindo averiguar os impactos que o clima global causa sobre a distribuição pluviométrica do local. Nesse contexto, o presente trabalho objetivou analisar IAC em quatro municípios de diferentes mesorregiões do Estado de Pernambuco, evidenciando os anos de seca e de chuva. Para realização desta pesquisa, foram selecionados os municípios: Barreiros, Caruaru, Floresta e Petrolina, das mesorregiões da Zona da Mata, Agreste, Sertão Pernambucano e Sertão do São Francisco, respectivamente. Os dados de precipitação das séries históricas de cada localidade foram obtidos na Agência Pernambucana de Águas e Clima. Com os dados, foram realizados os cálculos do IAC positivo e negativo, com base na normal climatológica e médias dos 10 anos de máximas e mínimas precipitações e, em sequência, houve a classificação de anos secos e úmidos de acordo com as classes de intensidade do IAC. Dentre os municípios analisados, Barreiros foi o mais úmido, reflexo da precipitação média anual (2026,79 mm) com o IAC variando entre -5,76 e 6,03. Para Caruaru, ocorreram 33 anos com desvios negativos e 25 com desvios positivos e o IAC variou entre -3,99 e 4,06. Em Floresta, o IAC variou entre -4,82 e 7,69 e ocorreu 1 ano extremamente úmido e 2 extremamente secos. E, em Petrolina, ocorreram 5 anos extremamente úmidos e 1 extremamente seco, sendo que o IAC oscilou entre 5,31 e 7,24. Conclui-se que a utilização do IAC, positivos e negativos, para as cidades analisadas, garante um resultado mais preciso do índice, refletindo em um planejamento agrícola mais elaborado para essas cidades. Sendo que a elevada quantidade de anos que apresentaram IAC negativo, para esses municípios, somada a significativa oscilação entre os anos úmidos e secos das series históricas, caracterizam essas localidades como instáveis para a produção agrícola de sequeiro.
The impact of the phosphorus limitation on denitrification efficiency and specific sludge production in industrial wastewater treatment
Authors: Stanek Radim , Zábranská Jana 
Published: September 2024

ABSTRACT

Heterotrophic denitrification is applied to the treatment of industrial wastewater from nitrated products with high concentrations of residual nitric and sulphuric acids and without organic matter. Wastewater with a concentration of nitrate nitrogen of 100–400 mg/L needs to be neutralized, methanol is used as an organic substrate, and phosphoric acid is used as a nutrient supplement. The pilot model of continuous denitrification was constructed with real wastewater influent. Experiments with different dosages of phosphorus 0–16.8 mg/g P/N-NOx were carried out to determine the effect of phosphorus limitation on the efficiency of denitrification. The minimum phosphorus concentration of 0.24 mg/L, representing a specific dose of P to remove oxidized nitrogen of 1.8 mg/g for sufficient denitrification efficiency was found after a sufficient time of adaptation of 70 days of the denitrification culture to low phosphorus concentrations in the reactor. Long-term successive reduction of phosphorus supply led to an increased proportion of organisms within the denitrifying sludge with lower phosphorus requirements. The results indicated that potential savings could be achieved by reducing phosphoric acid demand although limited phosphorus supply did not reduce specific sludge production.
Features of Gene Regulation in Violation of the Inflammatory
Response of Monocyte-like Cells Bearing Mitochondrial Mutations
Associated with Atherosclerosis
Authors: Orekhov Alexander N. , Nikiforov Nikita G. , Zhuravlev Alexander D. , Verkhova Svetlana S. , Omelchenko Andrey V. , Borodko Daria D. , Sukhorukov Vasily N. , Sinyov Vasily V. , Sobenin Igor A. 
Published: September 2024

Aim:
This research aimed to study the features of gene regulation of the inflammatory response in cells carrying mitochondrial mutations associated with atherosclerosis.

Background:
Inflammation plays an important, if not decisive, role in the occurrence of
atherosclerotic lesions and then accompanies it throughout its further development. Thus,
atherogenesis is a chronic inflammatory process. Chronification of inflammation is a consequence of disruption of the normal inflammatory response at the cell level of the vascular
wall.

Objectives:
In this study, we used cytoplasmic hybrids or cybrids carrying atherosclerosis-associated mitochondrial mutations to study gene regulation of inflammatory response.
The main goal of the study was to identify the key genes responsible for the impaired inflammatory response revealed for some cybrids.

Methods:
Inflammatory stimulation of cybrids was induced with bacterial
lipopolysaccharide, and assessed through secretion of pro-inflammatory cytokines CCL2,
IL8, IL6, IL1b. A transcriptome analysis was performed to identify the key genes (master
regulators) in the normal (tolerant) and intolerant response of cybrid cells

Results:
Normal inflammatory response after re-stimulation elicited a much smaller secretion of pro-inflammatory cytokines. In an intolerant response, the level of secretion upon
re-stimulation was the same or even higher than after the first stimulation. Normal and intolerant responses differed significantly both in terms of the number of signaling pathways
involved and qualitatively, since the signaling pathways for normal and intolerant responses are completely different. Master regulators controlling normal and intolerant inflammatory response were identified. For a normal response to the first inflammatory stimulation, no
common master up-regulators and 3 master down-regulators were identified. The reverse situation was observed with the intolerant inflammatory response: 6 master up-regulators,
and no master down regulators were identified. After the second inflammatory stimulation,
no master regulator common to all studied cytokines was found. Thus, key genes involved
in the development of intolerant inflammatory response have been identified. In addition,
other key genes were identified that were initially associated with an intolerant inflammatory response and thus determine disorders of the inflammatory reaction leading to chronification of inflammation.

Conclusion:
We identified disturbances in gene associated with the development of intolerant immune response that may be relevant to atherosclerosis. Key genes responsible for the
chronification of inflammation were discovered.
Single-Cell RNA Sequencing Revealing that MMP12+ Macrophages
are Associated with Cancer Liver Metastasis
Authors: Fan Li , Wang Qiang , Qian Qing , Wang Qing , Liu Dan , Gong Yuzhu , Xiong Zhiyong 
Published: September 2024

Aim:
We aimed to explore the MMP12+ macrophages function in liver metastasis
of Colorectal cancer [CRC].

Background:
CRC has a high incidence, and a great many patients develop liver metastases.
Some studies have found that macrophages may participate in the liver metastasis
of CRC.

Objective:
This study aimed to determine the factors and major signaling pathways of
MMP12+ macrophages affecting liver metastasis of CRC.

Methods:
The single-cell RNA sequencing [scRNA-seq] data of CRC and bulk transcriptome
data were downloaded. After filtering scRNA-seq data, dimensionality reduction
and clustering were performed to identify different cell subgroups. The FindAll-
Markers function was used to calculate the differentially expressed genes in each cell
subgroup, and the genes in the promising set were uploaded to the DAVID database to
analyze the biological processes to which these genes were enriched. Differentially expressed
genes among macrophage subgroups were selected by the AverageExpression
function. Then, the CIBERSORT algorithm was used to compute the proportion of each
macrophage subgroup in each bulk tissue and determine the most significant
macrophage subgroup. The dynamic changes of gene expression in macrophage subgroup
were computed by Pseudotime. Finally, CellChat was applied to investigate the
effect of the macrophage subgroup on epithelial cells and the ligand-receptor effect of
B cells and T cells.

Results:
Clustering scRNA-seq data showed a larger proportion of macrophages in liver
metastases. The proportion of MMP12+ macrophage subtypes increased gradually
among normal, tumor, and liver metastasis groups, and MMP12+ macrophages were associated
with angiogenesis, cell migration, and inhibited T cell proliferation. The Pseudotime
showed higher expression levels of genes related to angiogenesis and enhanced
TGF-β signaling pathway and the negative regulation of T cell proliferation with the occurrence
of liver metastasis in MMP12+ macrophages. MMP12+ macrophages can promote
the proliferation of epithelial cells and inhibit the activation of T cells and B cells.

Conclusion:
MMP12+ macrophages promoted liver metastasis of CRC by influencing
angiogenesis, TGF-β signaling pathway expression, and regulation of T cells and B
cells.
Therapeutic Correlation of TLR-4 Mediated NF-κB Inflammatory Pathways in Ischemic Injuries
Authors: Sharma Veerta , Sharma Prateek , Singh Thakur Gurjeet 
Published: September 2024

:
Ischemia-reperfusion (I/R) injury refers to the tissue damage that happens when blood
flow returns to tissue after a period of ischemia. I/R injuries are implicated in a large array of
pathological conditions, such as cerebral, myocardial, renal, intestinal, retinal and hepatic ischemia.
The hallmark of these pathologies is excessive inflammation. Toll-like receptors (TLRs)
are recognized as significant contributors to inflammation caused by pathogens and, more recently,
inflammation caused by injury. TLR-4 activation initiates a series of events that results in activation
of nuclear factor kappa-B (NF-κB), which stimulates the production of pro-inflammatory
cytokines and chemokines, exacerbating tissue injury. Therefore, through a comprehensive review
of current research and experimentation, this investigation elucidates the TLRs signalling pathway
and the role of TLR-4/NF-κB in the pathophysiology of I/R injuries. Furthermore, this review
highlights the various pharmacological agents (TLR-4/NF-κB inhibitors) with special emphasis on
the various ischemic injuries (cerebral, myocardial, renal, intestinal, retinal and hepatic). Future research
should prioritise investigating the specific molecular pathways that cause TLR-4/NF-κBmediated
inflammation in ischemic injuries. Additionally, efforts should be made to enhance treatment
approaches in order to enhance patient outcomes.
Physical and Functional Properties of Poly (Lactic Acid)/Paprika Oleoresin Films for Food Packaging Application
Authors: Pens Cristian , Venzke Klug Tâmmila , Santiago Tupuna Yerovi Diego , Flôres Simone , de Oliveira Rios Alessandro 
Published: September 2024

Background:
Paprika oleoresin is a compound extracted from paprika, with functional
properties, mainly due to its fatty acid composition and carotenoid profile. The effect of these
compounds on PLA composite films has not been investigated.

Objective:
The objective of this study is to evaluate the effect of paprika oleoresin at different
concentrations on the surface color, UV barrier, mechanical barrier, water vapor barrier, hydrophobicity,
paprika oleoresin release and antibacterial properties of PLA-based composite films.

Method:
Functional poly(lactic acid)-based films incorporating paprika oleoresin extract (POE) at
three levels (0.01%, 0.03% and 0.06%) were prepared using a solution casting method.

Results:
The paprika oleoresin showed an excellent compatibility with the PLA, as a uniform
dispersion was observed. Moreover, the addition of POE improved properties such as elasticity
(increase of 62%) and water vapor permeability (decrease of 69%). However, compared to the
control film (polylactic acid), the solubility of the polylactic acid and paprika oleoresin extract
films was found to be significantly increased. As the POE content increased, the color intensity of
the PLA films became lighter orange-yellow. The film composed by PLA/ POE (0.06%) also presented
significant bacterial inhibition for Staphylococcus aureus. In addition, the films demonstrated
total carotenoid release in food-simulating liquids.

Conclusion:
Functional PLA/POE films can be a good alternative to replace traditional food
packaging.