bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of simple sequence repeat markers for sweetpotato weevil resistance
Authors: Yada Benard , Alajo Agnes , Ssemakula Gorrettie N. , Brown-Guedira Gina , Otema Milton Anyanga , Stevenson Philip C. , Mwanga Robert O. M. , Craig Yencho G. 
Published: June 2017

AbstractThe development of sweetpotato [Ipomoea batatas(L.) Lam] germplasm with resistance to sweetpotato weevil (SPW) requires an understanding of the biochemical and genetic mechanisms of resistance to optimize crop resistance. The African sweetpotato landrace, ‘New Kawogo’, was reported to be moderately resistant to two species of SPW,Cylas puncticollisandCylas brunneus. Resistance has been associated with the presence of hydroxycinnamic acids esters (HCAs), but the underlying genetic basis remains unknown. To determine the genetic basis of this resistance, a bi-parental sweetpotato population from a cross between the moderately resistant, white-fleshed ‘New Kawogo’ and the highly susceptible, orange-fleshed North American variety ‘Beauregard’ was evaluated for SPW resistance and genotyped with simple sequence repeat (SSR) markers to identify weevil resistance loci. SPW resistance was measured on the basis of field storage root SPW damage severity and total HCA ester concentrations. Moderate broad sense heritability (H2 = 0.49) was observed for weevil resistance in the population. Mean genotype SPW severity scores ranged from 1.0 to 9.0 and 25 progeny exhibited transgressive segregation for SPW resistance. Mean genotype total HCA ester concentrations were significantly different (P < 0.0001). A weak but significant correlation (r = 0.103, P = 0.015) was observed between total HCA ester concentration and SPW severity. A total of five and seven SSR markers were associated with field SPW severity and total HCA ester concentration, respectively. Markers IBS11, IbE5 and IbJ544b showed significant association with both field and HCA-based resistance, representing potential markers for the development of SPW resistant sweetpotato cultivars.
Screening for pollen tolerance to high temperatures in tomato
Authors: Paupière Marine J. , van Haperen Pauline , Rieu Ivo , Visser Richard G. F. , Tikunov Yury M. , Bovy Arnaud G. 
Published: June 2017

AbstractAmong the abiotic stresses affecting plant reproduction, high temperature is one of the most prominent ones because it directly affects fruit set. So far, little attention has been paid to the investigation of the variation in high temperature tolerance among wild tomato (Solanum lycopersicum) germplasm. The objective of this study was to determine the tolerance of 17 different cultivated and wild tomato accessions to high temperature, using a pollen viability screening approach. Each of the 17 genotypes of tomato was analysed for their pollen quality under a 32 °C (day)/26 °C (night) regime. The total number of pollen per flower and the fraction of viable pollen were recorded. The number of pollen per flower varied between 35,547 and 109,490 whereas the fraction of viable pollen varied between 0.03 and 0.71. No correlation was found between these two traits. However, the combination of these traits could provide the best reproductive capability under high temperature. In this study, thermo-tolerant (LA2854, LA1478 and LA0417) as well as thermo-sensitive (LA1719, LA1580, and SWEET4) genotypes have been identified. Those genotypes can be used as novel genetic resources to get more insight into pollen thermo-tolerance mechanisms and be included in breeding programs.
Service development accounts for an even smaller share of European R&D investments than we may think
Authors: Broström Anders , Giertz Eric 
Published: June 2017

AbstractIn academic research on private R&D investments, sectoral differences are generally analyzed on the basis of firms’ classification according to systems such as the American NAICS and the European NACE industry classification systems. The same classification principles are applied in many countries and regions when aggregating R&D statistics to the level of industries, for example by the EU’s statistical office Eurostat. We report evidence suggesting that the share of R&D associated with development of service-oriented products or with service activities is systematically lower than the share of R&D conducted by service sector firms. Results from a survey run in Sweden shows that the revenue streams in a significant share of R&D-performing service sector firms (as classified according to NACE) are dominated by sales of physical products (e.g. factoryless goods producers). An even larger share report that a majority of their R&D is directed towards development of physical products rather than service products (e.g. consultancy companies acting as external R&D departments for their clients in manufacturing industries). These findings imply that the share of R&D investments focusing on service-oriented activities is even lower than traditional statistics suggest.
Nonlinear modulation of interacting between COMT and depression on brain function
Authors: Gong L. , He C. , Yin Y. , Ye Q. , Bai F. , Yuan Y. , Zhang H. , Lv L. , Zhang H. , Zhang Z. , Xie C. 
Published: June 2017

AbstractBackground:The catechol-O-methyltransferase (COMT) gene is related to dopamine degradation and has been suggested to be involved in the pathogenesis of major depressive disorder (MDD). However, how this gene affects brain function properties in MDD is still unclear.Methods:Fifty patients with MDD and 35 cognitively normal participants underwent a resting-state functional magnetic resonance imaging scan. A voxelwise and data-drive global functional connectivity density (gFCD) analysis was used to investigate the main effects and the interactions of disease states and COMT rs4680 gene polymorphism on brain function.Results:We found significant group differences of the gFCD in bilateral fusiform area (FFA), post-central and pre-central cortex, left superior temporal gyrus (STG), rectal and superior temporal gyrus and right ventrolateral prefrontal cortex (vlPFC); abnormal gFCDs in left STG were positively correlated with severity of depression in MDD group. Significant disease × COMT interaction effects were found in the bilateral calcarine gyrus, right vlPFC, hippocampus and thalamus, and left SFG and FFA. Further post-hoc tests showed a nonlinear modulation effect of COMT on gFCD in the development of MDD. Interestingly, an inverted U-shaped modulation was found in the prefrontal cortex (control system) but U-shaped modulations were found in the hippocampus, thalamus and occipital cortex (processing system).Conclusion:Our study demonstrated nonlinear modulation of the interaction between COMT and depression on brain function. These findings expand our understanding of the COMT effect underlying the pathophysiology of MDD.
Downregulation of miR-503 Promotes ESCC Cell Proliferation, Migration, and Invasion by Targeting Cyclin D1
Authors: Jiang Lanfang , Zhao Zitong , Zheng Leilei , Xue Liyan , Zhan Qimin , Song Yongmei 
Published: June 2017

Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers in China, but the underlying molecular mechanism of ESCC is still unclear. Involvement of microRNAs has been demonstrated in cancer initiation and progression. Despite the reported function of miR-503 in several human cancers, its detailed anti-oncogenic role and clinical significance in ESCC remain undefined. In this study, we examined miR-503 expression by qPCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues. Further investigation in the effect of miR-503 on ESCC cell proliferation, migration, and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype. Our study further identified CCND1 as the target gene of miR-503. Thus, miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1.
Regulatory effects of miR‐146a/b on the function of endothelial progenitor cells in acute ischemic stroke in mice
Authors: Su Zeng‐Feng , Sun Zhong‐Wu , Zhang Ying , Wang Shu , Yu Qi‐Gui , Wu Ze‐Bing 
Published: June 2017

AbstractThe study aims to explore how microRNA‐146a/b (miR‐146a/b) regulates the function of endothelial progenitor cells (EPCs) in acute ischemic stroke in mice. Eighty male SPF C57BL/6J mice were evenly divided into the model‐6 h, model‐12 h, model‐24 h (mice suffered from middle cerebral artery occlusion [MCAO] for 6 h, 12 h and model‐24 h) and normal groups. EPCs were transfected and assigned into the control, MCAO, MCAO‐miR‐146a, MCAO‐miR‐146b and MCAO‐miR‐146a/b groups. The qRT‐PCR was used to detect miR‐146a/b expression in EPCs. Expressions of tumor necrosis factor receptor‐associated factor 6 (TRAF6) and interleukin‐1 receptor‐associated kinase 1 (IRAK1) were detected using western blotting. Cell proliferation and migration of EPCs were testified using CCK‐8 assay and scratch test, respectively. Angiogenesis ability of EPCs was observed under microscope. MiR‐146a and miR‐146b expressions were lower in the model groups than the normal group. There were up‐regulated TRAF6 and IRAK1 expressions in the model‐6 h, model‐12 h and model‐24 h groups compared with the normal group. And there were down‐regulated TRAF6 and IRAK1 expressions in the MCAO‐miR‐146a, MCAO‐miR‐146b and MCAO‐miR‐146a/b groups than in the MCAO group. Compared with the control group, the proliferation, migration and angiogenesis ability of EPCs were significantly lower in the MCAO group, but higher in the MCAO‐miR‐146a, MCAO‐miR‐146b and MCAO‐miR‐146a/b groups. Besides, the miR‐146a/b group showed more enhancement than the MCAO‐miR‐146a and MCAO‐miR‐146b groups. MiR‐146a/b could down‐regulate the TRAF6 and IRAK1 expressions and promote proliferation, migration and angiogenesis ability of EPCs, which was important for recovery of patients with hyperacute ischemic stroke.
The Role of Low‐Intensity Pulsed Ultrasound on Cartilage Healing in Knee Osteoarthritis: A Review
Authors: Rothenberg Joshua B. , Jayaram Prathap , Naqvi Usker , Gober Joslyn , Malanga Gerard A. 
Published: June 2017

AbstractUltrasound (US) is a therapeutic modality that has been used in the treatment of musculoskeletal conditions for decades. In recent years, there have been technological advancements using low‐intensity pulsed ultrasound (LIPUS) as a clinical modality. The purpose of this review was to critically examine the medical literature to determine the effects of LIPUS on the chondrogenic properties of knee osteoarthritis. A literature search of 3 major databases (PubMed, Scopus, and EMBASE) was performed. Two independent physician reviewers screened titles and abstracts, yielding a total of 18 relevant articles after the inclusion and exclusion criteria were applied. Results favored that LIPUS has a promising effect on the cellular elements in articular cartilage, specifically on chondrocytes in knee osteoarthritis. Although the use of LIPUS is encouraging based on basic science and preclinical data, there is a paucity of evidence with respect to humans. Consequently, there is insufficient evidence to recommend for or against LIPUS in clinical OA populations. We suggest future directions for research centered on LIPUS in both human and animal models to delineate the effect on the biologic properties of cartilage in knee osteoarthritis.Level of EvidenceIII
Publication trends in obstructive sleep apnea: Evidence of need for more evidence
Authors: Araslanova R. , Paradis J. , Rotenberg B.W. 
Published: June 2017

ObjectivePublished research in obstructive sleep apnea (OSA) appears limited despite OSA being a highly prevalent adult and pediatric disease leading to many adverse outcomes if left untreated. We aimed to quantify the deficit in OSA scientific literature in order to provide a novel way of identifying gaps in knowledge and a need for further research inquiry.MethodsThis was a Bibliometric analysis study. Using Ovid Medline database we analyzed and compared research output (medical and surgical) between adult OSA and similarly prevalent chronic conditions (Type II diabetes (T2DM), coronary artery disease (CAD) and osteoarthritis (OA)) from December 2016 up to fifty years prior. Linear graphs were utilized to trend collected data. Utilizing same strategy, we compared publication trends for pediatric OSA to asthma and gastroesophageal reflux (GER).ResultsAdult OSA publications (n = 9314) were significantly underrepresented when compared to T2DM (n = 66,023), CAD (n = 31,526) and OA (n = 34,123). Linear plots demonstrated that despite increasing number of publications this disparity persisted annually. Surgical literature composed 10.4% (n = 972) of adult OSA publications and reached a plateau in the last ten years. Pediatric OSA (n = 2994) had less research output when compared to asthma (n = 47,442) and GER (n = 6705). However, over past five years pediatric OSA surpassed GER in annual number of publications. Surgical literature represented 23.1% (n = 693) of pediatric OSA publications and continued increasing over past ten years. Study methodologies for both adult and pediatric OSA showed a lack of randomized controlled trials and meta‐analyses in comparison to other diseases.ConclusionOur review shows substantial deficit in total, annual and surgical adult OSA published research compared to similarly prevalent diseases. This trend is not entirely observed in pediatric OSA literature.
Design of a 2.5‐kW L‐band solid‐state pulsed power amplifier for radar applications
Authors: Mohadeskasaei Seyed Alireza , Lin Fuhong , Zhou Xianwei , Abdullahi Sani Umar 
Published: June 2017

A compact design for a 2.5 kW solid‐state power amplifier (PA) based on 330‐W laterally diffused metal–oxide semiconductor transistors over 1.2–1.4 GHz is proposed. The design procedure is started with the design and implementation of a 330‐W unit amplifier (UA) independently. Optimum load and source impedances of the UA are obtained by pulling the source and load impedances with the aid of the simulator utility provided in Advanced Design System. The input–output matching networks are designed and implemented using multi‐section microstrip transmission lines. In order to construct a 2.5‐kW high PA (HPA), the eight pieces of designed UA are combined. A low‐loss 1–8 way planar binary power divider with an 8–1 way planar binary power combiner is used to divide and combine the microwave signals through eight UAs. The HPA delivers 63.7–64.3 dBm output power with 16.7–17.3 dB power gain and 55–60.4% power‐added efficiency over 1.2–1.4 GHz. To the best of the authors' knowledge, the most compact HPA design using packaged transistor at L‐band with such high output power level ever reported is presented.
Extraneural Supratentorial Primitive Neuroectodermal Tumor Metastasis: An Adult Case Report
Authors: Matar Nidhal , Bahri Kamel , Kallel Jallel , Boubaker Adnene , Jemel Hafedh 
Published: June 2017

AbstractExtraneural metastases of supratentorial primitive neuroectodermal tumors (PNET) are very rare and have been reported in only sporadic cases. We present a patient with supratentorial PNET metastasizing to the lung, diagnosed 4 months after cerebral surgery.
Lower Limb Deformity Analysis and the Planning of an Osteotomy
Authors: Schröter Steffen , Ateschrang Atesch , Ihle Christoph , Stöckle Ulrich , Dickschas Jörg , Harrer Jörg , Elson David 
Published: June 2017

AbstractThis review article provides an overview of lower limb deformity analysis. A brief history and radiographic standards are presented. Deformity analysis is considered in the frontal, sagittal, and axial planes, from simple to more complex cases. The authors recommended analysis methods based on their own experience of deformity correction, while acknowledging that there may be different ways to treat individual cases. Subsequently, methods for osteotomy planning to address the identified deformities are discussed.
Reliability of Roentgenographic Knee Alignment Measurements in Gonarthrosis
Authors: Huang Teng-Le , Wang Chen-Chie , Yang Kai-Chiang , Wu Chang-Chin 
Published: June 2017

AbstractA reliable method of measuring knee alignment is critical in the preoperative planning of high tibial osteotomy (HTO). This radiological study, based on the measurements of the mechanical and anatomical axes of the lower limb, aimed to determine which method would be more reliable and reproducible. From 2004 to 2013, 50 consecutive patients (50 knees) with medial gonarthrosis were treated with valgus-producing HTO. Four independent investigators assessed preoperative standing lower limb radiography (including hip, knee, and ankle joints) to measure both anatomical and mechanical axes. We defined a response as a difference in the angle measured by the same investigator using the same method. The covariates were the investigators and methods. To account for both inter- and intraobserver variabilities, all data were evaluated using a generalized estimating equation model. The results revealed that the effect of the investigators was not statistically significant; however, the effect of the measurement method was highly significant (p < 0.0001). The estimated coefficient of the anatomical axis method was negative, indicating that the reproducibility of the anatomical axis method was better than that of the mechanical axis.
A Posttraumatic Distal Radius Allograft: 10 Years Follow-Up
Authors: Biondi Marco , Roselli Giuliana , Bigazzi Prospero , Lauri Giulio 
Published: June 2017

Background Wrist osteoarthritis is a common disease often resulting from malunited fractures of the distal radius. The primary treatment purpose is to provide pain relief, while maintaining strength and mobility whenever possible. In a patient presenting a posttraumatic degeneration of the wrist, deciding which surgical technique, which joints to sacrifice and which to preserve is crucial to optimizing the outcome.
Case Description We describe a 10-year follow-up of an osteoarticular allograft of the distal radius proposed to treat an isolated distal radius posttraumatic degeneration. The patient was young and active. No or slight articular degeneration of the carpal rows was present. The surgical technique was based on a periarticular step-cut that allowed the preservation of the dorsal capsule and the maximum contact between the bone and the allograft.
Literature Review In the literature, the allograft of the radius is described as a solution to an oncologic problem. Considering a posttraumatic scenario other techniques are usually performed. However, compared with artificial interbody fusion devices and prosthetic implants, structural bone allograft retains an advantage in biologic performance. It has osteoconductive properties and is similar to native tissues providing a progressive incorporation by the host.
Clinical Relevance This surgical technique provide a metaphyseal step-cut able to ensure a secure stabilization and a wide contact surface between the allograft and the native bone. Integration of the allograft with good functional and radiological outcome seen after 10 years supports this technique.
A Guideline for the Diagnosis of Pediatric Mitochondrial Disease: The Value of Muscle and Skin Biopsies in the Genetics Era
Authors: Mayr Johannes , Nuoffer Jean , Prokisch Holger , Sperl Wolfgang , Wortmann Saskia 
Published: June 2017

AbstractMitochondrial diseases are highly heterogeneous on the clinical, biochemical, and genetic level. In the traditional diagnostic approach (“biopsy first”) the evaluation of the affected individual and his body fluids, combined with the analysis of the respiratory chain enzymes in muscle based the subsequent Sanger sequencing of single candidate genes (“from function to gene”). Within the past few years, next-generation sequencing techniques of leucocyte-derived DNA (e.g., exome sequencing), with a diagnostic yield of more than 40%, have become the first line routine technology. This implicates that the invasive muscle biopsy is performed less often, especially in children. Furthermore, in this “genetics-first” approach the detection of new candidate genes precedes functional evaluations (“from gene to function”) leading to reverse phenotyping of affected individuals. Here, we line out the value of muscle and other tissue biopsies in this “genetics-first” era. We describe when and why it is still needed. We create awareness of pitfalls in the genetic diagnostics of mitochondrial diseases still necessitating tissue biopsies. Finally, we discuss why tissue biopsies are required for confirmatory diagnostics, or for getting a biochemical diagnosis in patients with hidden variants not detectable by standard genetics.
Effekte der telefonischen Nachsorge in der onkologischen Rehabilitation nach Brustkrebs – Ergebnisse einer randomisierten Studie
Authors: Hass H. , Muthny F. , Stepien J. , Lerch J. , von der Marwitz C. , Schröck R. , Berger D. , Tripp J. 
Published: June 2017

Zusammenfassung
Hintergrund Die Effekte und Evidenz der onkologischen Rehabilitation und von Nachsorgeprogrammen nach onkologischer Erkrankung werden unverändert kontrovers beurteilt. Ziel dieser Studie war daher die Evaluation der Durchführbarkeit und der Nutzen einer telefonischen Nachsorge zur Verbesserung von therapeutischen Effekten nach onkologischer Rehabilitation.
Methodik 172 Brustkrebspatientinnen (27–54 Jahre) wurden zu Beginn der stationären Rehabilitation (T1), zu Reha-Ende (T2) und 6 Monate nach Reha-Ende (T3) (Kontroll-Gruppe ohne, Interventionsgruppe mit telefonischer Nachsorge in 4-wöchigen Abständen) mit einem Fragebogenpaket untersucht (u. a. IRES-24. HADS, LZI, Emotional Thermometer, Fragebogen „Return-to-work).
Ergebnisse Für den IRES-24 ergab sich in der 2-faktoriellen Varianzanalyse ein signifikanter Haupteffekt für den Messzeitpunkt (F(2,116)=40,49, p<0,01), ebenso für den HADS-Angst- sowie Depressionswert (F(2,117)=31,50, p<0,01; (F(2,11 6)=31,19, p<0,01), jedoch keine signifikanten Unterschiede in Abhängigkeit von der Intervention (KG vs. NG; (F(2,116)=3,03, p=0,059).
Schlussfolgerung Eine Verbesserung der Wirkung durch die telefonische Nachsorge ließ sich nicht belegen. Ursächlich hierfür könnte neben einer zu geringen Dosis der Intervention auch die in den letzten Jahren schon umgesetzten multimodalen Therapiekonzepte in der onkologischen Rehabilitation sein.
Aktuelle Diagnostik bei choroidalen Nävi
Authors: Lüke J. , Grisanti S. , Tura A. 
Published: June 2017

ZusammenfassungTrotz einer Vielfalt technischer Hilfsmittel zur Dokumentation und Unterstützung der Diagnosesicherung bleibt weiterhin die Abgrenzung zwischen kleinen uvealen Melanomen und großen Nävi schwierig. In vielen Fällen werden nur sorgfältige regelmäßige Kontrollen zum Ausschluss eines Wachstums bzw. zur Feststellung eines unveränderten Erscheinungsbilds empfohlen. In diesem Artikel werden unter Berücksichtigung der aktuellen Literatur die verschiedenen diagnostischen Techniken zur Dokumentation und Differenzierung von choroidalen Nävi vorgestellt.
Beeinflussen Gewürze den Blutdruck oder die Endothelfunktion?
Authors: Uehleke Bernhard 
Published: June 2017

Referiert
Azimi P, Ghiasvand R, Feizi A et al. Effect of cinnamon, cardamom, saffron and ginger consumption on blood pressure and a marker of endothelial function in patients with type 2 diabetes mellitus: A randomized controlled clinical trial. Blood Press 2016; 25: 133–140
Der Echte Eibisch Althaea officinalis L.
Authors: Reinelt Natalie , Melzig Matthias 
Published: June 2017

Zusammenfassung
Althaea officinalis L. (Malvaceae) ist v. a. in Asien und Europa anzutreffen. Die Blatt- und Wurzeldrogen werden gegen Reizhusten sowie Schleimhautentzündungen im Mund- und Rachenraum angewendet. Auch zur Behandlung einer leichten Gastritis können sie eingesetzt werden. Zu den Hauptinhaltsstoffen zählen Schleimstoffe und Flavonoide wie z. B. Tilirosid. Sie sind für antiinflammatorische, antioxidative, antimikrobielle, antitussive sowie wundheilungsfördernde Wirkungen verantwortlich. Darüber hinaus sind antihypertensive und hepatoprotektive Effekte berichtet worden.