bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impairment of Coronary Endothelial Function by Hypoxia-Reoxygenation Involves TRPC3 Inhibition-mediated KCa Channel Dysfunction: Implication in Ischemia-Reperfusion Injury
Authors: Wang Xiang-Chong , Sun Wen-Tao , Fu Jie , Huang Jun-Hao , Yu Cheuk-Man , Underwood Malcolm John , He Guo-Wei , Yang Qin 
Published: July 2017

AbstractDespite increasing knowledge of the significance of calcium-activated potassium (KCa) and canonical transient receptor potential (TRPC) channels in endothelial physiology, no studies so far have investigated the link between these two distinct types of channels in the control of vascular tone in pathological conditions. We previously demonstrated that hypoxia-reoxygenation (H-R) inhibits endothelial KCa and TRPC3 channels in porcine coronary arteries (PCAs). The present study further investigated whether modulation of TRPC3 is involved in H-R-induced KCa channel inhibition and associated vasodilatory dysfunction using approaches of wire myography, whole-cell voltage-clamp, and coimmunoprecipitation. Pharmacological inhibition or siRNA silencing of TRPC3 significantly suppressed bradykinin-induced intermediate- and small-conductance KCa (IKCa and SKCa) currents in endothelial cells of PCAs (PCAECs). TRPC3 protein exists in physical association with neither IKCa nor SKCa. In H-R-exposed PCAECs, the response of IKCa and SKCa to bradykinin-stimulation and to TRPC3-inhibition was markedly weakened. Activation of TRPC3 channels restored H-R-suppressed KCa currents in association with an improved endothelium-derived hyperpolarizing factor (EDHF)-type vasorelaxation. We conclude that inhibition of TRPC3 channels contributes to H-R-induced suppression of KCa channel activity, which serves as a mechanism underlying coronary endothelial dysfunction in ischemia-reperfusion (I-R) injury and renders TRPC3 a potential target for endothelial protection in I-R conditions.
Dynamics of high viscosity contrast confluent microfluidic flows
Authors: Kurdzinski Michael E. , Gol Berrak , Hee Aaron Co , Thurgood Peter , Zhu Jiu Yang , Petersen Phred , Mitchell Arnan , Khoshmanesh Khashayar 
Published: July 2017

AbstractThe laminar nature of microfluidic flows is most elegantly demonstrated via the confluence of two fluids forming two stable parallel flows within a single channel meeting at a highly stable interface. However, maintenance of laminar conditions can become complicated when there is a large viscosity contrast between the neighbouring flows leading to unique instability patterns along their interface. Here, we study the dynamics of high viscosity contrast confluent flows – specifically a core flow made of highly viscous glycerol confined by sheath flows made of water within a microfluidic flow focusing system. Our experiments indicate the formation of tapered core structures along the middle of the channel. Increasing the sheath flow rate shortens the tapered core, and importantly induces local instability patterns along the interface of core-sheath flows. The dynamics of such tapered core structures is governed by the intensity of instability patterns and the length of the core, according to which the core structure can experience stable, disturbed, broken or oscillated regimes. We have studied the dynamics of tapered core structures under these regimes. In particular, we have analysed the amplitude and frequency of core displacements during the broken core and oscillating core regimes, which have not been investigated before.
Carrier dynamics of Mn-induced states in GaN thin films
Authors: Chen Yu-Ting , Yang Chi-Yuan , Chen Po-Cheng , Sheu Jinn-Kong , Lin Kung-Hsuan 
Published: July 2017

AbstractGaN-based materials are widely used for light emission devices, but the intrinsic property of wide bandgap makes it improper for photovoltaic applications. Recently, manganese was doped into GaN for absorption of visible light, and the conversion efficiency of GaN-based solar cells has been greatly improved. We conducted transient optical measurements to study the carrier dynamics of Mn-doped GaN. The lifetime of carriers in the Mn-related intermediate bands (at 1.5 eV above the valence band edge) is around 1.7 ns. The carrier relaxation within the Mn-induced bandtail states was on the order of a few hundred picoseconds. The relaxation times of different states are important parameters for optimization of conversion efficiency for intermediate-band solar cells.
Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis
Authors: Jiang Yixiang , He Lianhua , Wu Pingfang , Shi Xiaolu , Jiang Min , Li Yinghui , Lin Yiman , Qiu Yaqun , Bai Fang , Liao Yiqun , Li Qingge , Zhang RenLi , Hu Qinghua 
Published: July 2017

AbstractPathogenic Vibrio spp., Aeromonas spp. and Plesiomonas shigelloides are associated with human gastroenteritis and wound infections, as well as fish diseases. The comprehensive and accurate identification of these pathogens is crucial for the current public health. The present study describes the development of a multiplex assay for the simultaneous identification of ten bacterial pathogens in a single reaction by using a multiplex ligation reaction based on probe melting curve analysis (MLMA). The specificity for target genes was 100%, as assessed with a panel of 67 bacterial pathogens, which indicated no cross-reactions. The detection limit of this assay ranged from 0.8 × 107 CFU/mL to 1.5 × 108 CFU/mL at the pure bacterial culture level and from 0.1 ng to 1.0 ng at the DNA level. The MLMA assay was used to detect ten species of pathogens in 269 clinical and seafood samples, and for further validation, the results were compared with the conventional culture method. The results indicated greater than 90% sensitivity and 100% specificity for each bacterial pathogen tested, and the kappa correlation for all the pathogens ranged from 0.95 to 1.00. Overall, this assay is well suited for public health laboratories for its high throughput, accuracy, and low cost.
On wakefulness fluctuations as a source of BOLD functional connectivity dynamics
Authors: Haimovici Ariel , Tagliazucchi Enzo , Balenzuela Pablo , Laufs Helmut 
Published: July 2017

AbstractHuman brain dynamics and functional connectivity fluctuate over a range of temporal scales in coordination with internal states and environmental demands. However, the neurobiological significance and consequences of functional connectivity dynamics during rest have not yet been established. We show that the coarse-grained clustering of whole-brain dynamic connectivity measured with magnetic resonance imaging reveals discrete patterns (dynamic connectivity states) associated with wakefulness and sleep. We validate this using EEG in healthy subjects and patients with narcolepsy and by matching our results with previous findings in a large collaborative database. We also show that drowsiness may account for previous reports of metastable connectivity states associated with different levels of functional integration. This implies that future studies of transient functional connectivity must independently monitor wakefulness. We conclude that a possible neurobiological significance of dynamic connectivity states, computed at a sufficiently coarse temporal scale, is that of fluctuations in wakefulness.
Nucleophile/Electrophile Combinations in Aromatic Substitution: From Wheland to Wheland–Meisenheimer Intermediates Using Strongly Activated Arenes
Authors: Micheletti Gabriele , Boga Carla 
Published: July 2017

This short review provides an overview on the interaction between 1,3,5-triaminobenzene derivatives and different kinds of electrophiles. Due to the ambident reactivity of these nucleophiles (i.e., at the nitrogen atom of the substituents and at the aromatic carbon atom) different compounds can be obtained. Particular attention is devoted to the detection, isolation, and characterization of covalent intermediates of aromatic substitution, starting from Wheland intermediates until the first detection and characterization of Wheland–Meisenheimer intermediates.1	Introduction2	Reactions between 1,3,5-Triaminobenzene Derivatives and Charged Electrophiles2.1	The Proton as an Electrophile2.2	Arenediazonium Salts as Electrophiles3	Reactions between 1,3,5-Triaminobenzene Derivatives and Neutral­ Electrophiles3.1	Alkyl Halides as Electrophiles3.2	Acyl Halides and Sulfonyl Chlorides as Electrophiles3.3	Aryl Halides and Heteroaryl Halides as Electrophiles3.4	Polynitroheteroaromatics as Electrophiles4	Conclusion
Outcomes of Infants Who Failed to Extubate despite Systemic Corticosteroids
Authors: Lewis Tamorah , Oschman Alexandra , Dai Hong , Brophy Katie , Norberg Michael , Nyp Michael , Truog William , Cuna Alain 
Published: July 2017

Objective The objective of this study was to assess the outcomes of preterm infants who failed to extubate following initial treatment with steroids.
Materials and Methods This is a retrospective cohort study of ventilator-dependent preterm infants treated with first course of systemic steroids to facilitate extubation. Outcomes of infants who successfully extubated were compared with infants who failed to extubate.
Results In this study, 74 infants (mean gestation 25.4 ± 1.4 weeks and mean birth weight 764 ± 163 g) met inclusion criteria. Of these, 41 (55%) were successfully extubated and 33 (45%) were not. Baseline demographics were similar between the two groups. The primary outcome of severe bronchopulmonary dysplasia (BPD) or death at 36 weeks was higher among infants who failed to extubate (94 vs. 63%, p = 0.002). Severe BPD remained significantly higher even after adjustment for potential confounders (odds ratio: 12.2, 95% confidence interval: 2.1–70.5, p = 0.005). Extubation failure was also associated with substantially higher rate of tracheostomy (32 vs. 5%, p = 0.003) and gastrostomy tube placement (61 vs. 22%, p = 0.001), as well as longer days of hospitalization (179 ± 72 vs. 129 ± 44 days, p = 0.001) and mechanical ventilation (112 ± 89 vs. 52 ± 42 days, p < 0.001).
Conclusion Failure to extubate after first course of systemic steroids for BPD is associated with poor prognostic implications.
RBP4/Lp-PLA2/Netrin-1 signaling regulation of cognitive dysfunction in diabetic nephropathy complicated with silent cerebral infarction
Authors: Chen Danyan , Huang Xiaolong , Lu Song , Deng Huacong , Gan Hua , Du Xiaogang , Weixue Tang 
Published: July 2017

Abstract
Aim To investigate the RBP4/Lp-PLA2/Netrin-1 signal regulation of cognitive function impairment in diabetic nephropathy patients with silent cerebral infarction (SCI).
Methods One-hundred patients newly diagnosed with diabetic nephropathy patients were included. The patients were divided into SCI group and NSCI group according to the radiological data. The degrees of cognitive dysfunction were evaluated. Serum RBP4 concentrations were determined by ELISA and protein expressions of Lp-PLA2 and Netrin-1 were determined by Western Blot.
Results Compared with NSCI group, the cognitive function of patients in SCI group was impaired, the concentrations of RBP4 and the expressions of Lp-PLA2 and Netrin-1 increased (P<0.05). RBP4 concentrations were positively correlated with the cognitive dysfunction of SCI patients. Moreover, there existed a regression correlation between them.
Conclusion RBP4 may be used as a predictive factor of diabetic nephropathy patients complicated with SCI and is positively correlated with cognitive dysfunction. RBP4/Lp-PLA2/Netrin-1 pathway activation may be one of the occurrence mechanisms in diabetic nephropathy complicated with SCI.
Switching from Premixed Insulin To Basal Insulin Analogue For Type 2 Diabetes and Role of Dipeptidyl Peptidase-4 Inhibitors
Authors: Gómez-Peralta Fernando , Abreu Cristina , Mora-Navarro Gustavo , López-Morandeira Pilar , Pérez-Gutierrez Esteban , Cordero-García Blanca , Brito-Sanfiel Miguel 
Published: July 2017

Abstract
Introduction This study aimed to confirm the usefulness of basal insulin analogue plus oral antidiabetic drugs (OADs) for type 2 diabetes (T2D) patients inadequately controlled with premixed insulin with/without OADs and assess the role of dipeptidyl peptidase-4 (DPP-4) inhibitors within this regimen in clinical practice.
Methods Spanish retrospective observational study that included 186 T2D patients with glycosylated hemoglobin (HbA1c) >7% (53 mmol/mol) despite premixed insulin with/without OADs who had been switched to basal insulin analogue plus OADs. Study data describing the situation before the treatment switch and 6 months later was retrospectively retrieved from patients’ medical charts.
Results Switching to a basal insulin plus OADs decreased HbA1c (−1.0%, p<0.001), fasting (−38.1 mg/dl, p<0.001) and postprandial glycemia (−36.1 mg/dl, p<0.001), with reduced body weight (−1.1 kg, p<0.001) and hypoglycemic episodes (−17.5%, p<0.001). 68 (36.6%) patients received a basal insulin plus DPP-4 inhibitor±metformin and 74 (39.8%) plus metformin only. The DPP-4 inhibitor±metformin group showed a greater HbA1c reduction than the metformin group (1.3±1.4% vs. 0.9±1.0%, p=0.022), with no significant differences between groups in hypoglycemic episodes.
Conclusions Basal insulin analogue plus OADs may be a useful treatment for type 2 diabetes patients inadequately controlled with premixed insulin. Administering DPP-4 inhibitors within this regimen may contribute to improve patients’ glycemia, with a favorable weight-change profile and without increasing hypoglycemia risk.
The chronicle of an ongoing crisis
Authors: Lykou Christina , Mitsikopoulou Bessie 
Published: July 2017

Abstract
This study explores media discourses and representations of Greece and Europe in the Greek press in the context of the recent socio-economic crisis. While several studies have explored the representation of Greece in the international press, very few have investigated the discursive construction of crisis in the Greek press. Theoretically and methodologically, the study draws on the tradition of critical discourse analysis, which it combines with corpus linguistics tools. The employed methodology is qualitative and interpretative in nature and attempts an analysis of media texts at a discoursal and grammatical level. The analysis is both synchronic and diachronic, following the development of the crisis through two newspapers, Kathimerini and Ta Nea, at different phases of the crisis.
The role of inferencing in the interpretation of two expressive speech act constructions
Authors: Panther Klaus-Uwe , Thornburg Linda L. 
Published: July 2017

Abstract
We analyze two illocutionary constructions, viz. an autonomous complement clause construction, instantiated by That it should come to this!, and a wh-interrogative construction, such as What do think you are doing? Both constructions convey emotive and evaluative senses and have a factual presupposition. Conceptually and pragmatically, they are members of a large family of expressive constructions, albeit morphosyntactically unrelated. The first construction is directly associated with a non-compositional illocutionary meaning. In contrast, the interpretation of the second construction requires a number of inferential steps leading from a still extant source meaning (neutral question) to a conventionalized indirect expressive and directive target meaning. The chapter finishes with some reflections on the relation between semantics and pragmatics as well as on the necessity of integrating an inferential component into cognitive linguistic theory.
Revisiting the English resultative family of constructions
Authors: Peña Cervel María Sandra 
Published: July 2017

Abstract
The resultative construction has spurred much debate over the last five decades. This proposal, which is embedded within the Cognitive Construction Grammar paradigm, provides a critical discussion on two fine-grained classifications of resultatives, Goldberg and Jackendoff’s (2004) and Luzondo’s (2014), in order to lay the foundations for a new taxonomy. The family of constructions put forward here benefits from both typologies by drawing on their main insights (the inclusion of the creation and transformation configurations as resultatives, or the constraining factors which license lexical constructional fusion) and by making up for some drawbacks (the status of the way construction as part of the broad family of resultatives) with the goal of achieving a greater degree of refinement and accuracy in the description of resultatives.
Effects of transition metal additions on double-oxide film defects in an Al–Si–Mg alloy
Authors: Griffiths W. D. , Caden A. J. , Chen Qi 
Published: July 2017

Double oxide film defects have been associated with reduced mechanical properties and increased variability in properties in cast Al alloys. This paper explores the effects of the addition of around 0.4–0.5 wt-% of the transition metals Mo or W to an Al–7Si–0.3Mg alloy (2L99). The variability of tensile properties was significantly reduced, resulting in an approximate doubling of the values of the Weibull modulus of the ultimate tensile strength, and an increase of 10–20% in the Weibull modulus of the %Elongation. Scanning electron microscopy examination of the fracture surfaces revealed the presence of oxide films, as expected, but also found AlN. The mechanism(s) by which Mo or W additions improve mechanical properties has not been established, but the presence of AlN suggested the accelerated consumption of the internal atmosphere of the double oxide film defects, leading to a reduction in their size and hence their deleterious effects. This paper is part of a thematic issue on Light Alloys.
Arctic marine conservation is not prepared for the coming melt
Authors: Harris Peter T , Macmillan-Lawler Miles , Kullerud Lars , Rice Jake C 
Published: July 2017

Abstract
As the summer minimum in Arctic sea ice cover reduces in area year by year due to anthropogenic global climate change, so interest grows in the un-tapped oil, gas and fisheries resources that were previously concealed beneath. We show that existing marine protected areas in the Arctic Ocean offer little or no protection to many habitats and deep seafloor features that coincide spatially with areas likely to be of interest to industry. These habitats are globally unique, hosting Arctic species within pristine environments that are currently undergoing rapid adjustment to climate-induced changes in ocean dynamics, species migration and primary production. They are invaluable as reference points for conservation monitoring and assessment. The existing Arctic marine protected area network needs to be expanded in order to protect these habitats and be fully coordinated with other spatial and non-spatial measures intended to protect Arctic habitats and ensure any uses of Arctic marine or subsea resources are sustainable.
Debating the effectiveness of marine protected areas
Authors: Pendleton Linwood H , Ahmadia Gabby N , Browman Howard I , Thurstan Ruth H , Kaplan David M , Bartolino Valerio 
Published: July 2017

Abstract
Increasing the size and number of marine protected areas (MPAs) is widely seen as a way to meet ambitious biodiversity and sustainable development goals. Yet, debate still exists on the effectiveness of MPAs in achieving ecological and societal objectives. Although the literature provides significant evidence of the ecological effects of MPAs within their boundaries, much remains to be learned about the ecological and social effects of MPAs on regional and seascape scales. Key to improving the effectiveness of MPAs, and ensuring that they achieve desired outcomes, will be better monitoring that includes ecological and social data collected inside and outside of MPAs. This can lead to more conclusive evidence about what is working, what is not, and why. Eight authors were asked to write about their experiences with MPA effectiveness. The authors were instructed to clearly define “effectiveness” and discuss the degree to which they felt MPAs had achieved or failed to be effective. Essays were exchanged among authors and each was invited to write a shorter “counterpoint.” The exercise shows that, while experiences are diverse, many authors found common ground regarding the role of MPAs in achieving conservation targets. This exchange of perspectives is intended to promote reflection, analysis, and dialogue as a means for improving MPA design, assessment, and integration with other conservation tools.
Cosmological constraints from a joint analysis of cosmic growth and expansion
Authors: Moresco M. , Marulli F. 
Published: July 2017

Abstract
Combining measurements on the expansion history of the Universe and on the growth rate of cosmic structures is key to discriminate between alternative cosmological frameworks and to test gravity. Recently, Linder proposed a new diagram to investigate the joint evolutionary track of these two quantities. In this letter, we collect the most recent cosmic growth and expansion rate data sets to provide the state-of-the-art observational constraints on this diagram. By performing a joint statistical analysis of both probes, we test the standard Λcold dark matter model, confirming a mild tension between cosmic microwave background predictions from Planck mission and cosmic growth measurements at low redshift (z &lt; 2). Then we test alternative models allowing the variation of one single cosmological parameter at a time. In particular, we find a larger growth index than the one predicted by general relativity $\gamma =0.65^{+0.05}_{-0.04}$. However, also a standard model with total neutrino mass of 0.26 ± 0.10 eV provides a similarly accurate description of the current data. By simulating an additional data set consistent with next-generation dark-energy mission forecasts, we show that growth rate constraints at z &gt; 1 will be crucial to discriminate between alternative models.
Day-to-day experience in resolution of pain after surgery
Authors: Houle Timothy T. , Miller Scott , Lang Jason E. , Booth Jessica L. , Curry Regina S. , Harris Lynnette , Aschenbrenner Carol A. , Eisenach James C. 
Published: July 2017

Abstract
We know little about the individual pain experience of patients recovering from surgery in the first weeks after hospital discharge. Here, we examine individual differences in the day-to-day experience after 2 major surgeries: lower limb total major joint arthroplasty (TJA) and cesarean delivery (CD). Fifty-five TJA patients and 157 CD patients were recruited to complete questionnaires and record their daily pain experiences after surgery. After hospital discharge, patients recorded their pain intensity once daily for 60 days (CD) or twice daily for 2 weeks, once daily for 2 weeks, weekly for 8 weeks, and monthly for 3 months (TJA). Pain scores were modeled using growth curve and Bayesian change-point models. Individual differences in the model fits were examined for evidence of day-to-day differences in pain. A log time model was the simplest model that fit the data, but examination of the residuals revealed high autocorrelation representing misspecification. A change-point model fit the data better and revealed that the form of recovery fundamentally changed between days 10 and 21 after surgery. These data add meaningfully to our understanding of recovery from pain after surgery by extending the period of frequent observations a few days after surgery to a 2-month period. These high time resolution data suggest that there is a typical experience of pain resolution after surgery, but that meaningful subpopulations of experience may exist. They also indicate that a transition occurs within 1 month after surgery from 1 pattern of change in pain over time to another.