bluuebunny/crossref_metadata_2025_split huggingface dataset:
Substanzkonsum in der Allgemeinbevölkerung in Deutschland
Authors: Matos Elena Gomes de , Atzendorf Josefine , Kraus Ludwig , Piontek Daniela 
Published: October 2016

Zusammenfassung. Ziel: Die Verbreitung des Substanzkonsums in der erwachsenen Allgemeinbevölkerung Deutschlands soll anhand aktueller Daten dargestellt werden. Methodik: Datengrundlage bildete der Epidemiologische Suchtsurvey 2015, eine bundesweite Befragung in der Wohnbevölkerung von 18 bis 64 Jahren (n = 9,204; Rücklaufquote: 52.2 %). Probanden wurden zum Gebrauch von Tabak, Alkohol, illegalen Drogen und Medikamenten befragt. Ergebnisse: In den letzten 30 Tagen hatten 28.7 % der Befragten Tabakprodukte genutzt. Den Konsum von Alkohol in diesem Zeitraum berichteten 72.8 %. Hinweise auf klinisch relevanten Konsum in den letzten 12 Monaten wiesen 28.3 % (Männer) bzw. 9.6 % (Frauen) auf. Die 12-Monats-Prävalenz illegaler Drogen lag bei 6.1 % für Cannabis und bei maximal 1 % für alle anderen Substanzen. Unter Medikamenten hatten Schmerzmittel die höchste Gebrauchsprävalenz (47.1 %) sowie die geringste Prävalenz täglichen Gebrauchs unter Nutzern (8.6 %). Schlussfolgerungen: Die Ergebnisse belegen die nach wie vor hohe Verbreitung des Substanzkonsums in der Allgemeinbevölkerung. Konsumprävalenz sowie Hinweise auf klinisch relevanten Konsum sind in Bezug auf legale Substanzen, die eine hohe Verfügbarkeit charakterisiert, am häufigsten. Hinweise auf klinisch relevanten Konsum illegaler Drogen bestanden bei deutlich weniger Personen. Da es sich dabei jedoch um eine besondere Risikogruppe handelt, ist deren Erreichung durch adäquate Angebote sicherzustellen.
Antenna-assisted picosecond control of nanoscale phase transition in vanadium dioxide
Authors: Muskens Otto L , Bergamini Luca , Wang Yudong , Gaskell Jeffrey M , Zabala Nerea , de Groot CH , Sheel David W , Aizpurua Javier 
Published: October 2016

AbstractNanoscale devices in which the interaction with light can be configured using external control signals hold great interest for next-generation optoelectronic circuits. Materials exhibiting a structural or electronic phase transition offer a large modulation contrast with multi-level optical switching and memory functionalities. In addition, plasmonic nanoantennas can provide an efficient enhancement mechanism for both the optically induced excitation and the readout of materials strategically positioned in their local environment. Here, we demonstrate picosecond all-optical switching of the local phase transition in plasmonic antenna-vanadium dioxide (VO2) hybrids, exploiting strong resonant field enhancement and selective optical pumping in plasmonic hotspots. Polarization- and wavelength-dependent pump–probe spectroscopy of multifrequency crossed antenna arrays shows that nanoscale optical switching in plasmonic hotspots does not affect neighboring antennas placed within 100 nm of the excited antennas. The antenna-assisted pumping mechanism is confirmed by numerical model calculations of the resonant, antenna-mediated local heating on a picosecond time scale. The hybrid, nanoscale excitation mechanism results in 20 times reduced switching energies and 5 times faster recovery times than a VO2 film without antennas, enabling fully reversible switching at over two million cycles per second and at local switching energies in the picojoule range. The hybrid solution of antennas and VO2 provides a conceptual framework to merge the field localization and phase-transition response, enabling precise, nanoscale optical memory functionalities.
Genomic heterogeneity of multiple synchronous lung cancer
Authors: Liu Yu , Zhang Jianjun , Li Lin , Yin Guangliang , Zhang Jianhua , Zheng Shan , Cheung Hannah , Wu Ning , Lu Ning , Mao Xizeng , Yang Longhai , Zhang Jiexin , Zhang Li , Seth Sahil , Chen Huang , Song Xingzhi , Liu Kan , Xie Yongqiang , Zhou Lina , Zhao Chuanduo , Han Naijun , Chen Wenting , Zhang Susu , Chen Longyun , Cai Wenjun , Li Lin , Shen Miaozhong , Xu Ningzhi , Cheng Shujun , Yang Huanming , Lee J. Jack , Correa Arlene , Fujimoto Junya , Behrens Carmen , Chow Chi-Wan , William William N. , Heymach John V. , Hong Waun Ki , Swisher Stephen , Wistuba Ignacio I. , Wang Jun , Lin Dongmei , Liu Xiangyang , Futreal P. Andrew , Gao Yanning 
Published: October 2016

AbstractMultiple synchronous lung cancers (MSLCs) present a clinical dilemma as to whether individual tumours represent intrapulmonary metastases or independent tumours. In this study we analyse genomic profiles of 15 lung adenocarcinomas and one regional lymph node metastasis from 6 patients with MSLC. All 15 lung tumours demonstrate distinct genomic profiles, suggesting all are independent primary tumours, which are consistent with comprehensive histopathological assessment in 5 of the 6 patients. Lung tumours of the same individuals are no more similar to each other than are lung adenocarcinomas of different patients from TCGA cohort matched for tumour size and smoking status. Several known cancer-associated genes have different mutations in different tumours from the same patients. These findings suggest that in the context of identical constitutional genetic background and environmental exposure, different lung cancers in the same individual may have distinct genomic profiles and can be driven by distinct molecular events.
Tuning thermal conductivity in molybdenum disulfide by electrochemical intercalation
Authors: Zhu Gaohua , Liu Jun , Zheng Qiye , Zhang Ruigang , Li Dongyao , Banerjee Debasish , Cahill David G. 
Published: October 2016

AbstractThermal conductivity of two-dimensional (2D) materials is of interest for energy storage, nanoelectronics and optoelectronics. Here, we report that the thermal conductivity of molybdenum disulfide can be modified by electrochemical intercalation. We observe distinct behaviour for thin films with vertically aligned basal planes and natural bulk crystals with basal planes aligned parallel to the surface. The thermal conductivity is measured as a function of the degree of lithiation, using time-domain thermoreflectance. The change of thermal conductivity correlates with the lithiation-induced structural and compositional disorder. We further show that the ratio of the in-plane to through-plane thermal conductivity of bulk crystal is enhanced by the disorder. These results suggest that stacking disorder and mixture of phases is an effective mechanism to modify the anisotropic thermal conductivity of 2D materials.
Discrete bisoliton fiber laser
Authors: Liu X. M. , Han X. X. , Yao X. K. 
Published: October 2016

AbstractDissipative solitons, which result from the intricate balance between dispersion and nonlinearity as well as gain and loss, are of the fundamental scientific interest and numerous important applications. Here, we report a fiber laser that generates bisoliton – two consecutive dissipative solitons that preserve a fixed separation between them. Deviations from this separation result in its restoration. It is also found that these bisolitons have multiple discrete equilibrium distances with the quantized separations, as is confirmed by the theoretical analysis and the experimental observations. The main feature of our laser is the anomalous dispersion that is increased by an order of magnitude in comparison to previous studies. Then the spectral filtering effect plays a significant role in pulse-shaping. The proposed laser has the potential applications in optical communications and high-resolution optics for coding and transmission of information in higher-level modulation formats.
The cytochrome bd-I respiratory oxidase augments survival of multidrug-resistant Escherichia coli during infection
Authors: Shepherd Mark , Achard Maud E. S. , Idris Adi , Totsika Makrina , Phan Minh-Duy , Peters Kate M. , Sarkar Sohinee , Ribeiro Cláudia A. , Holyoake Louise V. , Ladakis Dimitrios , Ulett Glen C. , Sweet Matthew J. , Poole Robert K. , McEwan Alastair G. , Schembri Mark A. 
Published: October 2016

AbstractNitric oxide (NO) is a toxic free radical produced by neutrophils and macrophages in response to infection. Uropathogenic Escherichia coli (UPEC) induces a variety of defence mechanisms in response to NO, including direct NO detoxification (Hmp, NorVW, NrfA), iron-sulphur cluster repair (YtfE), and the expression of the NO-tolerant cytochrome bd-I respiratory oxidase (CydAB). The current study quantifies the relative contribution of these systems to UPEC growth and survival during infection. Loss of the flavohemoglobin Hmp and cytochrome bd-I elicit the greatest sensitivity to NO-mediated growth inhibition, whereas all but the periplasmic nitrite reductase NrfA provide protection against neutrophil killing and promote survival within activated macrophages. Intriguingly, the cytochrome bd-I respiratory oxidase was the only system that augmented UPEC survival in a mouse model after 2 days, suggesting that maintaining aerobic respiration under conditions of nitrosative stress is a key factor for host colonisation. These findings suggest that while UPEC have acquired a host of specialized mechanisms to evade nitrosative stresses, the cytochrome bd-I respiratory oxidase is the main contributor to NO tolerance and host colonisation under microaerobic conditions. This respiratory complex is therefore of major importance for the accumulation of high bacterial loads during infection of the urinary tract.
Integrating Candida albicans metabolism with biofilm heterogeneity by transcriptome mapping
Authors: Rajendran Ranjith , May Ali , Sherry Leighann , Kean Ryan , Williams Craig , Jones Brian L. , Burgess Karl V. , Heringa Jaap , Abeln Sanne , Brandt Bernd W. , Munro Carol A. , Ramage Gordon 
Published: October 2016

AbstractCandida albicans biofilm formation is an important virulence factor in the pathogenesis of disease, a characteristic which has been shown to be heterogeneous in clinical isolates. Using an unbiased computational approach we investigated the central metabolic pathways driving biofilm heterogeneity. Transcripts from high (HBF) and low (LBF) biofilm forming isolates were analysed by RNA sequencing, with 6312 genes identified to be expressed in these two phenotypes. With a dedicated computational approach we identified and validated a significantly differentially expressed subnetwork of genes associated with these biofilm phenotypes. Our analysis revealed amino acid metabolism, such as arginine, proline, aspartate and glutamate metabolism, were predominantly upregulated in the HBF phenotype. On the contrary, purine, starch and sucrose metabolism was generally upregulated in the LBF phenotype. The aspartate aminotransferase gene AAT1 was found to be a common member of these amino acid pathways and significantly upregulated in the HBF phenotype. Pharmacological inhibition of AAT1 enzyme activity significantly reduced biofilm formation in a dose-dependent manner. Collectively, these findings provide evidence that biofilm phenotype is associated with differential regulation of metabolic pathways. Understanding and targeting such pathways, such as amino acid metabolism, is potentially useful for developing diagnostics and new antifungals to treat biofilm-based infections.
Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124
Authors: Dong Peixin , Xiong Ying , Watari Hidemichi , Hanley Sharon JB , Konno Yosuke , Ihira Kei , Suzuki Fumihiko , Yamada Takahiro , Kudo Masataka , Yue Junming , Sakuragi Noriaki 
Published: October 2016

AbstractDerepression of wild-type p53 by suppressing its negative inhibitor iASPP (Inhibitor of apoptosis-stimulating protein of p53) represents a potential therapeutic option for cervical cancer (CC). Here, we reported a novel functional significance of iASPP upregulation in cervical tumorigenesis: iASPP acts as a key promoter of CC cell proliferation, epithelial-mesenchymal transition, invasion and cancer stemness, by interacting with p53 to suppress p53-mediated transcription of target genes and reducing p53-responsive microRNA-34a levels. Moreover, we demonstrate that miR-124, directly targeting iASPP, reduces expression of iASPP and attenuates CC cell growth and invasiveness. Low miR-124 expression is inversely correlated with increased expression of iASPP mRNA in CC tissues. In a cohort of 40 patients with CC, the low miR-124 expression was correlated with poor 5-year overall survival (P = 0.0002) and shorter disease-free survival 5-year (P = 0006). Treatment with the DNA methyltransferase inhibitor Zebularine increases miR-124 expression and retards CC cell growth and invasion with minimal toxicity to normal cells. Even at a non-toxic concentration, Zebularine was effective in suppressing CC cell invasion and migration. Altogether, the restoration of miR-124 reduces iASPP expression and leads to p53-dependent tumor suppression, suggesting a therapeutic strategy to treat iASPP-associated CC.
Hypoxia-inducible factor-2 alpha promotes the proliferation of human placenta-derived mesenchymal stem cells through the MAPK/ERK signaling pathway
Authors: Zhu Chengxing , Yu Jiong , Pan Qiaoling , Yang Jinfeng , Hao Guangshu , Wang Yingjie , Li Lanjuan , Cao Hongcui 
Published: October 2016

AbstractHuman placenta-derived mesenchymal stem cells (hPMSCs) reside in a physiologically low-oxygen microenvironment. Hypoxia influences a variety of stem cell cellular activities, frequently involving hypoxia-inducible factor-2 alpha (HIF-2α). This research showed that hPMSCs cultured in hypoxic conditions (5% O2) exhibited a more naïve morphology and had a higher proliferative capability and higher HIF-2α expression than hPMSCs cultured in normoxic conditions (21% O2). Similar to the hypoxic cultures, hPMSCs over-expressing HIF-2α showed higher proliferative potential and higher expression of CCND1 (CyclinD1), MYC (c-Myc), POU5F1 (Oct4) and the components of the MAPK/ERK pathway. In contrast, these genes were down-regulated in the HIF-2α-silenced hPMSCs. After adding the MAPK/ERK inhibitor PD0325901, cell growth and the expression of CCND1 and MYC were inhibited. Furthermore, the chromatin immunoprecipitation (ChIP) assay and electrophoretic mobility shift assay (EMSA) showed that HIF-2α bound to the MAPK3 (ERK1) promoter, indicative of its direct regulation of MAPK/ERK components at the transcriptional level during hPMSC expansion. Taken together, our results suggest that HIF-2α facilitated the preservation of hPMSC stemness and promoted their proliferation by regulating CCND1 and MYC through the MAPK/ERK signaling pathway.
Rising soil temperature in China and its potential ecological impact
Authors: Zhang Hui , Wang Enli , Zhou Daowei , Luo Zhongkui , Zhang Zhengxiang 
Published: October 2016

AbstractGlobal warming influences a series of ecological processes and ecosystems’ stability. Although comprehensive studies have been done to investigate responses of various ecosystem processes to rising air temperatures, less is known about changes in soil temperatures and their impact on below-ground processes, particularly in deep layers. Herein, we used 50 y of temperature data (1962–2011) from 360 sites in China to assess spatio-temporal changes in soil temperatures from the surface to a depth of 3.20 m. We determined, apparently for the first time, that soil surface temperature increased 31% more than air temperature, potentially leading to more carbon release to the atmosphere than predicted. Annual mean surface temperature increased by 2.07–4.04 and 0.66–2.21 °C in northern and southern China, respectively, with the greatest in winter. Warming occurred as deep as 3.20 m. The soil temperature rise was predicted to have increased soil respiration by up to 28%, reinforcing climate warming and extending the potential growing season by up to 20 d across China. However, use of only air temperature to estimate soil temperature changes would underestimate those impacts. In conclusion, these results highlighted the importance of soil warming and of using soil temperature to assess and predict soil processes.
Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique
Authors: Jiang Chao , Yuan Yuan , Yang Guang , Jin Yan , Liu Libing , Zhao Yuyang , Huang Luqi 
Published: October 2016

AbstractInaccurate labeling of materials used in herbal products may compromise the therapeutic efficacy and may pose a threat to medicinal safety. In this paper, a rapid (within 3 h), sensitive and visual colorimetric method for identifying substitutions in terminal market products was developed using cationic conjugated polymer-based fluorescence resonance energy transfer (CCP-based FRET). Chinese medicinal materials with similar morphology and chemical composition were clearly distinguished by the single-nucleotide polymorphism (SNP) genotyping method. Assays using CCP-based FRET technology showed a high frequency of adulterants in Lu-Rong (52.83%) and Chuan-Bei-Mu (67.8%) decoction pieces, and patented Chinese drugs (71.4%, 5/7) containing Chuan-Bei-Mu ingredients were detected in the terminal herbal market. In comparison with DNA sequencing, this protocol simplifies procedures by eliminating the cumbersome workups and sophisticated instruments, and only a trace amount of DNA is required. The CCP-based method is particularly attractive because it can detect adulterants in admixture samples with high sensitivity. Therefore, the CCP-based detection system shows great potential for routine terminal market checks and drug safety controls.
Magnetomitotransfer: An efficient way for direct mitochondria transfer into cultured human cells
Authors: Macheiner Tanja , Fengler Vera Heike Ingeborg , Agreiter Marlene , Eisenberg Tobias , Madeo Frank , Kolb Dagmar , Huppertz Berthold , Ackbar Richard , Sargsyan Karine 
Published: October 2016

AbstractIn the course of mitochondrial diseases standard care mostly focuses on treatment of symptoms, while therapeutic approaches aimed at restoring mitochondrial function are currently still in development. The transfer of healthy or modified mitochondria into host cells would open up the possibilities of new cell therapies. Therefore, in this study, a novel method of mitochondrial transfer is proposed by anti-TOM22 magnetic bead-labeled mitochondria with the assistance of a magnetic plate. In comparison to the passive transfer method, the magnetomitotransfer method was more efficient at transferring mitochondria into cells (78–92% vs 0–17% over 3 days). This transfer was also more rapid, with a high ratio of magnetomitotransferred cells and high density of transferred mitochondria within the first day of culture. Importantly, transferred mitochondria appeared to be functional as they strongly enhanced respiration in magnetomitotransferred cells. The novel method of magnetomitotransfer may offer potential for therapeutic approaches for treatment of a variety of mitochondria-associated pathologies, e.g. various neurodegenerative diseases.
Ternary mixtures of ionic liquids for better salt solubility, conductivity and cation transference number improvement
Authors: Karpierz E. , Niedzicki L. , Trzeciak T. , Zawadzki M. , Dranka M. , Zachara J. , Żukowska G. Z. , Bitner-Michalska A. , Wieczorek W. 
Published: October 2016

AbstractWe hereby present the new class of ionic liquid systems in which lithium salt is introduced into the solution as a lithium cation−glyme solvate. This modification leads to the reorganisation of solution structure, which entails release of free mobile lithium cation solvate and hence leads to the significant enhancement of ionic conductivity and lithium cation transference numbers. This new approach in composing electrolytes also enables even three-fold increase of salt concentration in ionic liquids.
Marginal evidence for cosmic acceleration from Type Ia supernovae
Authors: Nielsen J. T. , Guffanti A. , Sarkar S. 
Published: October 2016

AbstractThe ‘standard’ model of cosmology is founded on the basis that the expansion rate of the universe is accelerating at present — as was inferred originally from the Hubble diagram of Type Ia supernovae. There exists now a much bigger database of supernovae so we can perform rigorous statistical tests to check whether these ‘standardisable candles’ indeed indicate cosmic acceleration. Taking account of the empirical procedure by which corrections are made to their absolute magnitudes to allow for the varying shape of the light curve and extinction by dust, we find, rather surprisingly, that the data are still quite consistent with a constant rate of expansion.
Detection of Gravitational Wave Emission by Supermassive Black Hole Binaries Through Tidal Disruption Flares
Authors: Hayasaki Kimitake , Loeb Abraham 
Published: October 2016

AbstractGalaxy mergers produce supermassive black hole binaries, which emit gravitational waves prior to their coalescence. We perform three-dimensional hydrodynamic simulations to study the tidal disruption of stars by such a binary in the final centuries of its life. We find that the gas stream of the stellar debris moves chaotically in the binary potential and forms accretion disks around both black holes. The accretion light curve is modulated over the binary orbital period owing to relativistic beaming. This periodic signal allows to detect the decay of the binary orbit due to gravitational wave emission by observing two tidal disruption events that are separated by more than a decade.
Recombinant Reg3β protein protects against streptozotocin-induced β-cell damage and diabetes
Authors: Luo Chen , Yu Lu-Ting , Yang Meng-Qi , Li Xiang , Zhang Zhi-Yuan , Alfred Martin O , Liu Jun-Li , Wang Min 
Published: October 2016

AbstractRegenerating genes (Reg) have been found during the search for factors involved in pancreatic islet regeneration. Our recent study discovered that pancreatic β-cell-specific overexpression of Reg3β protects against streptozotocin (Stz) -induced diabetes in mice. To investigate its potential roles in the treatment of diabetes, we produced a recombinant Reg3β protein and provided evidence that it is active in promoting islet β-cell survival against Stz- triggered cell death. Though ineffective in alleviating preexisting diabetes, pretreatment of recombinant Reg3β was capable of minimizing the Stz-induced hyperglycemia and weight loss, by preserving serum and pancreatic insulin levels, and islet β-cell mass. No obvious changes were observed in the rate of cell proliferation and hypertrophy in α- or acinar-cells after treatment with recombinant Reg3β. The underlying mechanism of Reg3β-mediated protection seems to involve Akt activation which upregulates Bcl-2 and Bcl-xL levels and consequently promotes cell survival.
Multi-Terminal Spin Valve on Channels with Spin-Momentum Locking
Authors: Sayed Shehrin , Hong Seokmin , Datta Supriyo 
Published: October 2016

AbstractIt is experimentally established that charge current flowing in a channel with spin-momentum locking such as topological insulator surface states or Rashba interfaces induces a spin voltage, which can be electrically measured with a ferromagnetic contact along the current path. Using this fact in conjunction with Onsager reciprocity arguments, we make the surprising prediction that the anti-parallel resistance of a spin valve can be either larger or smaller than the parallel resistance depending on the direction of spin flow relative to the direction of spin-momentum locking. However, we argue that this remarkable signature of spin-momentum locking can only be observed in multi-terminal measurements. Two-terminal measurements in the linear response regime, will show a single anti-parallel resistance larger than the parallel resistance as commonly observed in channels without spin-orbit coupling. We support this result with detailed numerical calculations based on a semiclassical model that provides insight into the underlying physics.