bluuebunny/crossref_metadata_2025_split huggingface dataset:
COLT-Viz: Interactive Visualization of Antibody Lineage Trees
Authors: He Chenfeng , Wendel Ben S. , Xiao Jun , Chen Keke , Jiang Ning 
Published: November 2017

AbstractMany tools have been developed to visualize phylogenetic trees, which is a traditional technique for evolutionary tree analysis. However, due to the unique characteristics of antibody lineage trees, the phylogenetic method cannot adequately construct proper tree structures for antibody lineages, and many other tools have been developed to address this problem. However, there still lacks of an adequate tool to visualize the resulted antibody lineage structures that are more complicated than phylogenetic trees. In addition, high-throughput sequencing-based antibody repertoire profiling enables the counting of the number of transcripts associated with individual antibody sequences, thus more dimensions need to be encoded in the tree structure visualization. Further, users may wish to manually adjust the tree structure for a special context. When doing so, they may wish to maintain some biological constraints that are applicable in antibody lineage tree structure, such as isotype switching constraints or different sampling constraints. Here, we report an interactive visualization tool (COLT-Viz) designed to display the number of RNA copies, number of somatic hypermutations, and sample collection time associated with each antibody sequence as well as the distance to neighboring sequences for each antibody sequence in the lineage. COLT-Viz also allows users to interactively visualize and edit antibody lineage structures while giving users the option to automatically check biological constraints on the edited structures to ensure accuracy. COLT-Viz takes JSON text format as input files and can easily be used to visualize networks with or without the biological constraints. We believe the amount of information that can be displayed for complex antibody lineages, the interactive interface, and the option of checking for biological constraints make COLT-Viz a versatile tool for antibody lineage tree visualization that will guide further biological discoveries.
Rapid, modular, and cost-effective generation of donor DNA constructs for CRISPR-based gene knock-in
Authors: Chen Yi-Jiun , Wang Weikang , Tian Xiao-Jiun , Lefever Daniel E. , Taft David A. , Xing Jianhua 
Published: November 2017

ABSTRACTCRISPR-based gene knock-in at endogenous sites is desirable in multiple fields such as quantitative studies of signal transduction pathways and gene regulation, synthetic biology, and disease modeling. Contrasting the knock-out procedure, a key step of CRISPR knock-in procedure relies on the homology-directed repairing (HDR) process that requires a donor construct as repair template. Therefore, it is desirable to generate a series of donor DNA constructs efficiently and cost-effectively. In this study, we developed a general Gibson assembly procedure that combines strengths of a Modular Overlap-Directed Assembly with Linkers (MODAL) strategy and a restriction enzyme based hierarchical framework. This procedure also allows fusing sgRNAs to the constructs for enhanced homology-directed repairing efficiency. Experimental tests on multiple constructs achieved from 3-8 folds of increase in assembly efficiency to high yield of constructs that failed to make with conventional Gibson assembly. The modularized procedure is simple, fast and cost-effective while making multiple constructs, and a computer package is provided for customized design.
Engaging workforce in downsized firms: scenario of the Indian IT/ITES sector
Authors: Tiwari Binita , Lenka Usha 
Published: November 2017

PurposeOrganisations increasingly reduce enterprise costs via cost cutting measures of downsizing/layoffs as a short-term strategy. The purpose of this paper is to illustrate the scenario of downsizing in the Indian IT/ITES sector and also identify enablers to develop and engage talent in downsized firms.Design/methodology/approachThis paper is a general review.FindingsDespite higher growth rates in the recent years, the IT/ITES sector faces an uncertain future due to economic recession, which has changed the business scenario across the globe. Organisations need to resuscitate employees’ confidence and self-efficacy through psychosocial support mechanisms like professional guidance by an emotionally intelligent leader, capacity-building measures, and continuous learning opportunities. They should prepare scheduled reviews by taking into account individual needs, learning styles, and current work priorities. Provisions for adequate pay, fringe benefits, job security, and healthy working conditions can further enhance morale and motivation of survivors.Originality/valueThis paper contributes to the literature of downsizing in the Indian context. Moreover, it emphasises on the significant roles of communication policies, knowledge sharing, and learning in rebuilding the psychological contract of employees.
Role conflict and ambivalence in the aged-parent-adult-child relationship
Authors: Yu Xinjia , Miao Chunyan , Leung Cyril , Salmon Charles Thomas 
Published: November 2017

Purpose
The parent-child relationship is important to the solidarity of families and the emotional well-being of family members. Since people are more dependent on their close social relationships as they age, understanding the quality of relationships between aged parents and their adult children is a critical topic. Previous research shows that this relationship is complicated with both kinship and ambivalence. However, there is little research on the causes of this complexity. This paper proposes a role model to explain this complexity by studying the leadership transition within a family as the child grows.

Design/methodology/approach
In this paper, we proposed a novel perception to understand this transition process and explain related problems based on the analysis of the leader-follower relationship between the parents and their children.

Findings
When a child is born, his/her parents become the leader of this family because of their abilities, responsibilities and the requirements of the infant. This leader-follower role structure will last a long time in this family. Decades later, when the parents become old and the child grows up, the inter-generational contracts within the family and the requirement of each members change. This transition weakens the foundation of the traditional leader-follower role structure within the family. If either the parent or the child does not want to accept their new roles, both of them will suffer and struggle in this relationship. This role conflict will cause ambivalence in the relationship between aged parents and their adult children.

Originality/value
Based on the quantitative study model provided in this paper, we can moderate the relationships between aged parents and their adult children. This effort is meaningful in enhancing the quality of life and emotional wellbeing for senior citizens.
How the Chinese really negotiate: observations from an Australian-Chinese trade negotiation
Authors: McColl Rod , Descubes Irena , Elahee Mohammad 
Published: November 2017

Purpose
Previous research suggests that negotiation style and conflict management strategies are influenced heavily by cultural factors. In the case of the Chinese, findings have largely produced stereotypical views about their behavior, but the authors argue that this position is becoming increasingly blurred in the global economy.

Design/methodology/approach
Data were collected using participant observation during negotiations of a free-trade agreement between China and Australia followed by in-depth interviews with Chinese delegates.

Findings
Consistent with Confucianism and a Taoism-based value system, there was evidence of strong cultural influence on conflict management approaches. Two a priori Chinese strategies were evident – avoidance and accommodating, with five tactics. However, contrary to previous research, the authors found use of two conflict management strategies normally associated with a western approach – competing and compromising, with five associated tactics.

Practical implications
Chinese negotiators are knowledgeable and capable of adopting western negotiation strategies and tactics. The authors advise managers involved in international negotiations with Chinese managers to be cautious when relying on historical stereotypical assessments and to think differently about the emerging Chinese negotiator.

Originality/value
Few published negotiation studies involve real negotiations based on actual observations, particularly in an international setting. Contrary to many published studies, we demonstrate that conflict management approaches used by Chinese negotiators have evolved into a blend of traditional Chinese and western styles.
Counting the spoons: what really influences corporate reputation
Authors: Hirsch Peter Buell 
Published: November 2017

Purpose
The purpose of this paper is to examine and assess the importance of corporate reputation as a tool of business strategy. The topic has been the subject of significant discussion particularly with respect to reputation rankings and metrics. The author wanted to assess the evidence for the importance of reputation to a company’s success and whether there were related activities that might be of equal or greater value.

Design/methodology/approach
The approach was to review some of the more prominent reputation rankings and metrics and, anecdotally, to assess the impact of negative reputation impacts on market share and revenues. The author also tried to identify other corporate marketing strategies that might be of greater value than a focus on reputation alone.

Findings
What the author has determined was that there would always be a place for opinion surveys of reputation and the rankings that go with them, as well as certain outcomes-based metrics with a reputation component. However, the author believes that comprehensive influencer engagement programs have the greater potential for a positive impact on a company’s business success while, at the same time, supporting its reputation.

Research limitations/implications
The findings’ principal limitations are the subjective nature of the review and evaluation which are based on the author’s 30-year career in helping companies manage their reputation.

Practical implications
The practical implications of the paper are that companies should pursue a balanced reputation strategy, not solely restricted to seeking rankings and awards but equally, if not more, focused on creating the kinds of influencer engagement that are a richer and truer source of long-term reputation.

Social implications
The author believes that by focusing on the needs and interests of real influencers as opposed to abstract opinion survey panels, companies will do much more concrete work that creates social as well as business value.

Originality/value
While a great deal has been written about reputation and trust, the author believes that this is the first attempt to connect reputation with influencer engagement as a strategy.
A critical assessment of work and family life of female entrepreneurs in Sub-Saharan Africa
Authors: Adom Kwame , Asare-Yeboa Irene Tiwaa , Quaye Daniel M. , Ampomah Abena Oforiwaa 
Published: November 2017

PurposeThe purpose of this paper is to critically evaluate how work and family lives of female entrepreneurs in emerging Sub-Saharan countries including Ghana influence their business performance, with focus on criteria such as income, number of outlets, and number of employees the entrepreneur controls.Design/methodology/approachA phenomenological qualitative approach to research was adopted for this study to help the researcher gain deeper understanding of experiences of Ghanaian female entrepreneurs with respect to their work and family life and its influence on business performance. With a purposive sampling technique, 25 active women entrepreneurs with family responsibilities were sampled for the study. A multiple case study strategy, in-depth face-to-face interviews, and participant observation were used to solicit responses from participants included in the study.FindingsThe study revealed that a majority of female entrepreneurs involved in the study believe that their roles and responsibilities to their families have a negative impact on both the income they generate from their business and their willingness to expand their outlets. However, it has a positive impact on their willingness to employ additional employees in their businesses. As a consequence, this study reveals that quality family life is essential to these female entrepreneurs and they are willing to put the well-being of their families first, even at the expense of their business growth.Research limitations/implicationsThe study focused solely on the work and family life of women entrepreneurs and its influence on their business performance in Ghana. However, there exist other critical issues that affect women entrepreneurs in the Sub-Saharan region that can be explored to provide more insight on this subject or in different contexts.Originality/valueAs a first of its kind in the Ghanaian context, little is known in extant literature until now with regard to work and family life of female entrepreneurs and its influence on their business performance. This study therefore seeks to bridge this gap.
City tourism and the sharing economy – potential effects of online peer-to-peer marketplaces on urban property markets
Authors: Brauckmann Stefan 
Published: November 2017

PurposeThe purpose of this paper is to investigate the potential effects of the so-called sharing economy on growing city tourism as well as on urban property markets.Design/methodology/approachOfficial statistical data and a geo-information system (GIS) are used on a small scale in order to identify concentration processes among overnight visitors and the potential concomitant conflicts with other interest groups.FindingsCurrently, the effects of the sharing economy on housing markets and city tourism are barely measurable and are limited to a few central locations. However, a growing demand can be discerned in housing-like accommodation concepts which can be operated via booking platforms. As there is likely to be strong future growth in this area, continuous market observation (monitoring) is urgently advised.Research limitations/implicationsOfficial statistics only allow an analysis of overnight guests staying with larger accommodation providers. Booking platforms for holiday homes and other temporary accommodation options have such little interest in data transparency that the overall phenomenon of city tourism can be addressed only in part.Practical implicationsAssociating various data within the GIS enables municipal administrators and urban planners to identify potential sources of conflict within the property markets in good time and effectively counteract these where possible.Social implicationsIncreases in property prices directly attributable to growing city tourism may lead to the displacement of less financially secure members of the established population as well as businesses.Originality/valueThe sharing economy is a relatively new research topic which will become increasingly important in future. The identification of potential sources of conflict due to tourist accommodation has therefore not yet been comprehensively carried out on a small scale.
A concise approach to eigenform and reflexivity
Authors: Kauffman Louis H. 
Published: November 2017

Purpose
This paper aims to introduce the concept and praxis for eigenform in the context of second-order cybernetics.

Design/methodology/approach
The paper is designed as a formal (partly mathematical) introduction with excursions into the applications and meanings of these constructions. Mathematics studies what a distinction would be if there could be a distinction. Mathematics is a special form of fictional design. This study raises the question of “What it would mean to go beyond mathematics or for mathematics to go beyond itself?”.

Findings
This study shows how objects in the author’s experience can be seen to be eigenforms and that in this context such objects are a construct of their interactions, linguistic and otherwise experiential. In this way, the author can investigate scientifically without the need for an assumption of objectivity. The author cocreates the universe through the discovery of distinctions and eigenforms in their dialogue with what can be.

Research limitations/implications
The implications of this research are profound for the performance and exploration of science. The author can explore their role in that creation and find that what they create is independent of significant subsets of their actions.

Practical implications
The practical implications of this study are strongest for the logical understanding of the author’s constructions and actions. They have used eigenform and reflexivity to maintain a clear view of their participation in their own worlds.

Social implications
The social implications are in accordance with the practical implications. The author can now admit that they each produce eigenform models of the others and for themselves. These models have in-depth usage, in that it is understood that one is not identical with their models.

Originality/value
This paper presents a highly original and very simple way to incorporate second-order cybernetics into all thought and action.
An examination of internal auditor turnover intentions
Authors: Mubako Grace , Mazza Tatiana 
Published: November 2017

PurposeThe purpose of this paper is to examine the factors that are associated with internal auditors’ professional turnover intentions.Design/methodology/approachThe study analyzes data from responses to the Institute of Internal Auditors’ (IIA) (2015) Common Body of Knowledge global survey and uses a multivariate approach to identify factors that influence internal auditor turnover intentions.FindingsResults show that internal auditor turnover intentions are negatively associated with an academic background in accounting, possessing internal audit professional certification, and having access to more training opportunities. Turnover intentions are positively associated with organizational-professional conflict, restricted access to documents and personnel, and the existence of a program of using the internal audit function as management training ground. Differences by IIA global region highlight the diversity in the turnover challenges that face the professional globally.Originality/valueResults from this study are important because they bring attention to issues that potentially lead to internal auditors leaving the profession. This can help the profession and organizations take measures to motivate internal auditors to remain in the profession and alleviate the current staffing challenges faced by the profession.
Multilevel influences of team identification and transactive memory on team effectiveness
Authors: Michinov Estelle , Juhel Jacques 
Published: November 2017

Purpose
The purpose of this study was to examine the mediating effect of transactive memory between team identification and two outcomes of team effectiveness (i.e. team member satisfaction and team performance).

Design/methodology/approach
Data were obtained from a survey among 502 employees working in 53 teams, and analyzed by Multilevel Structural Equation Modeling.

Findings
Results showed that transactive memory partially mediated the relationship between team identification and team effectiveness at the individual level. Moreover, transactive memory, specifically the coordination component, fully mediated the relationship between team identification and team effectiveness at the team level.

Research limitations/implications
The study used a cross-sectional design for the questionnaire and no objective measure of team performance.

Practical implications
Managers who want to develop effective work teams may be advised to organize team-building activities to strengthen both affective and cognitive aspects.

Originality value
This is the first empirical study to examine the relationships between team identification, transactive memory and team effectiveness from a multilevel perspective.
Macro‐anatomical and morphometric studies of the hindlimb of grasscutter (Thryonomys swinderianus, Temminck‐1827)
Authors: Onwuama K. T. , Ojo S. A. , Hambolu J. O. , Dzenda T. , Zakari F. O. , Salami S. O. 
Published: November 2017

SummaryThe hindlimb of the grasscutter (Thryonomys swinderianus, Temminck‐1827) was studied using 12 adult (≥6 months) rats of either sex with the mean weights of 1.42 ± 0.20 kg and 0.82 ± 0.13 kg for buck (n = 6) and doe (n = 6), respectively (p < .05). Characteristics of the bones were studied by gross observation after their preparation. Measurements of different segments of the hindlimb were also taken, giving a total average length of 31.80 cm. Correlation coefficients between length of bone and weight of rats were positive (p < .05) in bucks for all bone segments, except the pes (p > .05), whereas those in does were all non‐significant (p > .05), with significant sexual dimorphism in the relationships. The average total number of bones in the hindlimb was 92, with no apparent sexual dimorphism. The bones of the hindlimb revealed important differences and similarities in morphology with those of other rodents. The Os coxae presented a relatively large obturator foramen, formed by the ramus of the ischium and shaft of the pubis. The femur had a body and two extremities with a prominent head, well‐defined neck and trochanter. The fibula, a slender bone with triangular‐shaped proximal extremity, ran down the length of tibia with proximal attachment (via cartilage) and distal fusion, leaving an extensive interosseus space. The pes revealed eight tarsals and four metatarsals; each of the metatarsals showed three phalanges.
A 3′ untranslated region polymorphism rs2304277 in the DNA repair pathway gene OGG1 is a novel risk modulator for urothelial bladder carcinoma
Authors: Ahmed Tayyaba , Nawaz Saira , Noreen Rabia , Bangash Kashif Sardar , Rauf Abdur , Younis Muhammad , Anwar Khursheed , Khawaja Muhammad Athar , Azam Maleeha , Qureshi Abid Ali , Akhter Saeed , Kiemeney Lambertus A. , Qamar Raheel , Ali Syeda Hafiza benish 
Published: November 2017

SummaryAltered DNA repair capacity may affect an individual's susceptibility to cancers due to compromised genomic integrity. This study was designed to elucidate the association of selected polymorphisms in DNA repair genes with urothelial bladder carcinoma (UBC). OGG1 rs1052133 and rs2304277, XRCC1 rs1799782 and rs25487, XRCC3 rs861539, XPC rs2228001, and XPD rs13181 were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) in 200 UBC cases and 200 controls.We found association of OGG1 rs2304277 [odds ratio (OR)GG = 3.55, 95% confidence interval (CI) = 1.79–7.06] and XPC rs2228001 (ORAC = 2.38, 95% CI = 1.43–3.94) with UBC. In stratified analysis with respect to smoking status, OGG1 rs2304277 and XPC rs2228001 exhibited increased risk in smokers [(rs2304277 ORGG = 4.96, 95% CI = 1.51–16.30) (rs2228001 ORAC = 2.19, 95% CI = 1.02–4.72)] as well as nonsmokers [(rs2304277 ORGG = 2.95, 95% CI = 1.26–6.90) (rs2228001 ORAC = 2.57, 95% CI = 1.31–5.04)]. These polymorphisms were also associated with both low‐grade [(rs2304277 ORGG = 3.73, 95% CI = 1.72–8.09) (rs2228001 ORAC = 2.18, 95% CI = 1.21–3.92)] and high‐grade tumors [(rs2304277 ORGG = 3.45, 95% CI = 1.52–7.80) (rs2228001 ORAC = 2.81, 95% CI = 1.48–5.33)] as well as with non–muscle‐invasive bladder cancer [(rs2304277 ORGG = 4.03, 95% CI = 1.87–8.67) (rs2228001 ORAC = 2.14, 95% CI = 1.20–3.81)] and muscle‐invasive bladder cancer [(rs2304277 ORGG = 3.06, 95%CI = 1.31–7.13) (rs2228001 ORAC = 2.95, 95%CI = 1.51–5.75)]. This is the first study on DNA repair gene polymorphisms and UBC in the Pakistani population. It identifies OGG1 rs2304277 and replicates XPC rs2228001 as significant modulators of UBC susceptibility.