bluuebunny/crossref_metadata_2025_split huggingface dataset:
A role for Sel-Plex™, a source of organic selenium in selenised yeast cell wall protein, as a factor that influences meat stability
Authors: Edens F. W. , Sefton A. E. 
Published: December 2016

SummarySelenium is an important mineral required in the antioxidant system in animals, which is involved with oxidative stability in tissues, particularly membranes, and is involved in various aspects of meat quality and stability on the shelf, due to its protective properties on lipids, preventing rancidity. Se can be supplied in an inorganic or chemically organic form, and it is well known that the latter has beneficial properties and improved functionality in physiological systems compared to the former. Research has shown that organic Se is associated with increased tenderness and the prevention of certain problems in pale exudative meat, discolouration and off-flavours and odours in meat, although this depends on other components of the antioxidant system, such as vitamin E, being present as well. The change in prominence of glutathione peroxidase forms in their interaction with vitamin E in cell membranes is also noted. The following review (the third in a series) details the research that has been conducted into the role of Se in meat stability and related factors, with specific focus on organic forms of Se, namely the commercial product Sel-Plex™(Alltech Inc, Nicholasville, KY, USA), which is derived from yeast and in which selenium replaces sulphur in methionine forming selenomethionine in yeast protein.
Cervicofacial emphysema following Harmonic scalpel tonsillectomy: case report and comprehensive review of the literature
Authors: Crosbie R A , Kunanandam T 
Published: December 2016

AbstractBackground:Tonsillectomy is one of the most common surgical procedures performed worldwide. There are a handful of common complications, with bleeding being the most feared; however, rarer complications can present to a wide range of medical professionals.Methods:A 12-year-old girl presented with cervicofacial emphysema following tonsillectomy. This paper discusses the case and the management adopted, and presents the findings of a comprehensive literature review.Results:The patient made a full recovery, and was discharged after 3 days following conservative management with intravenous broad-spectrum antibiotics and supplemental low-flow oxygen.Conclusion:This paper presents the first reported case of cervicofacial emphysema following Harmonic scalpel tonsillectomy. Although this is an exceptionally rare complication, it is potentially serious and warrants further description to improve awareness.
How does relationship-based governance accommodate new entrants? Evidence from the cycle-rickshaw rental market
Authors: JAIN TARUN , SOOD ASHIMA 
Published: December 2016

AbstractRelationship-based contract enforcement is commonly thought to limit market expansion. In contrast, this paper illustrates how relationship-based contract governance accommodates new entrants into market exchange using a case study of the cycle-rickshaw rental market in a city in central India. Migrants face a higher penalty for default that introduces a gap between the ex ante risk for out-of-network agents and the ex post risk. As a result, cycle-rickshaw owners are more likely to rent to migrants and migrants are more likely to participate in rental contracts. With primary data on multidimensional measures of migrant status, we confirm that migrant status is a significant predictor of rental contract participation, even controlling for other variables that moderate the rickshaw driver's ability to own a cycle-rickshaw. Our findings thus introduce a new perspective into current understandings of relationship-based contract governance.
Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes
Authors: Cheeseman Liam P. , Boulanger Jérôme , Bond Lisa M. , Schuh Melina 
Published: December 2016

AbstractAn egg must be fertilized by a single sperm only. To prevent polyspermy, the zona pellucida, a structure that surrounds mammalian eggs, becomes impermeable upon fertilization, preventing the entry of further sperm. The structural changes in the zona upon fertilization are driven by the exocytosis of cortical granules. These translocate from the oocyte’s centre to the plasma membrane during meiosis. However, very little is known about the mechanism of cortical granule translocation. Here we investigate cortical granule transport and dynamics in live mammalian oocytes by using Rab27a as a marker. We show that two separate mechanisms drive their transport: myosin Va-dependent movement along actin filaments, and an unexpected vesicle hitchhiking mechanism by which cortical granules bind to Rab11a vesicles powered by myosin Vb. Inhibiting cortical granule translocation severely impaired the block to sperm entry, suggesting that translocation defects could contribute to miscarriages that are caused by polyspermy.
The origin of ultrahigh piezoelectricity in relaxor-ferroelectric solid solution crystals
Authors: Li Fei , Zhang Shujun , Yang Tiannan , Xu Zhuo , Zhang Nan , Liu Gang , Wang Jianjun , Wang Jianli , Cheng Zhenxiang , Ye Zuo-Guang , Luo Jun , Shrout Thomas R. , Chen Long-Qing 
Published: December 2016

AbstractThe discovery of ultrahigh piezoelectricity in relaxor-ferroelectric solid solution single crystals is a breakthrough in ferroelectric materials. A key signature of relaxor-ferroelectric solid solutions is the existence of polar nanoregions, a nanoscale inhomogeneity, that coexist with normal ferroelectric domains. Despite two decades of extensive studies, the contribution of polar nanoregions to the underlying piezoelectric properties of relaxor ferroelectrics has yet to be established. Here we quantitatively characterize the contribution of polar nanoregions to the dielectric/piezoelectric responses of relaxor-ferroelectric crystals using a combination of cryogenic experiments and phase-field simulations. The contribution of polar nanoregions to the room-temperature dielectric and piezoelectric properties is in the range of 50–80%. A mesoscale mechanism is proposed to reveal the origin of the high piezoelectricity in relaxor ferroelectrics, where the polar nanoregions aligned in a ferroelectric matrix can facilitate polarization rotation. This mechanism emphasizes the critical role of local structure on the macroscopic properties of ferroelectric materials.
A bromodomain–DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT
Authors: Miller Thomas C. R. , Simon Bernd , Rybin Vladimir , Grötsch Helga , Curtet Sandrine , Khochbin Saadi , Carlomagno Teresa , Müller Christoph W. 
Published: December 2016

AbstractBromodomains are critical components of many chromatin modifying/remodelling proteins and are emerging therapeutic targets, yet how they interact with nucleosomes, rather than acetylated peptides, remains unclear. Using BRDT as a model, we characterized how the BET family of bromodomains interacts with site-specifically acetylated nucleosomes. Here we report that BRDT interacts with nucleosomes through its first (BD1), but not second (BD2) bromodomain, and that acetylated histone recognition by BD1 is complemented by a bromodomain–DNA interaction. Simultaneous DNA and histone recognition enhances BRDT’s nucleosome binding affinity and specificity, and its ability to localize to acetylated chromatin in cells. Conservation of DNA binding in bromodomains of BRD2, BRD3 and BRD4, indicates that bivalent nucleosome recognition is a key feature of these bromodomains and possibly others. Our results elucidate the molecular mechanism of BRDT association with nucleosomes and identify structural features of the BET bromodomains that may be targeted for therapeutic inhibition.
Tremor activity inhibited by well-drained conditions above a megathrust
Authors: Nakajima Junichi , Hasegawa Akira 
Published: December 2016

AbstractTremor occurs on megathrusts under conditions of near-lithostatic pore-fluid pressures and extremely weakened shear strengths. Although metamorphic reactions in the slab liberate large amounts of fluids, the mechanism for enhancing pore-fluid pressures along the megathrust to near-lithostatic values remains poorly understood. Here we show anti-correlation between low-frequency earthquake (LFE) activity and properties that are markers of the degree of metamorphism above the megathrust, whereby LFEs occur beneath the unmetamorphosed overlying plate but are rare or limited below portions that are metamorphosed. The extent of metamorphism in the overlying plate is likely controlled by along-strike contrasts in permeability. Undrained conditions are required for pore-fluid pressures to be enhanced to near-lithostatic values and for shear strength to reduce sufficiently for LFE generation, whereas well-drained conditions reduce pore-fluid pressures at the megathrust and LFEs no longer occur at the somewhat strengthened megathrust. Our observations suggest that undrained conditions are a key factor for the genesis of LFEs.
Linking genomic reorganization to tumor initiation via the giant cell cycle
Authors: Niu N , Zhang J , Zhang N , Mercado-Uribe I , Tao F , Han Z , Pathak S , Multani A S , Kuang J , Yao J , Bast R C , Sood A K , Hung M-C , Liu J 
Published: December 2016

AbstractTo investigate the mechanisms underlying our recent paradoxical finding that mitotically incapacitated and genomically unstable polyploid giant cancer cells (PGCCs) are capable of tumor initiation, we labeled ovarian cancer cells with α-tubulin fused to green fluorescent protein, histone-2B fused to red fluorescent protein and FUCCI (fluorescent ubiquitination cell cycle indicator), and tracked the spatial and time-dependent change in spindle and chromosomal dynamics of PGCCs using live-cell fluorescence time-lapse recording. We found that single-dose (500 nm) treatment with paclitaxel paradoxically initiated endoreplication to form PGCCs after massive cell death. The resulting PGCCs continued self-renewal via endoreplication and further divided by nuclear budding or fragmentation; the small daughter nuclei then acquired cytoplasm, split off from the giant mother cells and acquired competency in mitosis. FUCCI showed that PGCCs divided via truncated endoreplication cell cycle (endocycle or endomitosis). Confocal microscopy showed that PGCCs had pronounced nuclear fragmentation and lacked expression of key mitotic proteins. PGCC-derived daughter cells were capable of long-term proliferation and acquired numerous new genome/chromosome alterations demonstrated by spectral karyotyping. These data prompt us to conceptualize a giant cell cycle composed of four distinct but overlapping phases, initiation, self-renewal, termination and stability. The giant cell cycle may represent a fundamental cellular mechanism to initiate genomic reorganization to generate new tumor-initiating cells in response to chemotherapy-induced stress and contributes to disease relapse.
KLF4 functions as an activator of the androgen receptor through reciprocal feedback
Authors: Siu M-K , Suau F , Chen W-Y , Tsai Y-C , Tsai H-Y , Yeh H-L , Liu Y-N 
Published: December 2016

AbstractIn prostate cancer, Krüppel-like factor 4 (KLF4) depletion occurs frequently, suggesting a role as suppressor tumor. KLF4 is a transcription factor associated with androgen receptor (AR) expression; however, its cellular functions and signaling regulation mechanism remain largely unknown. In this study, we demonstrated that activated AR binds to the KLF4 promoter and enhances KLF4 expression, which reciprocally targets the AR promoter, thus sustaining KLF4 activity. Ectopic KLF4 expression in androgen-independent prostate cancer cells induced AR expression and decreased cell proliferation, invasion and bone metastasis. We previously showed that increased microRNA (miR)-1 expression is associated with reduced bone metastasis of prostate cancer cells. Here we observed that KLF4 targets the primary miR-1-2 stem-loop promoter and stimulates miR-1 expression. In clinical prostate cancer specimens, KLF4 levels were positively correlated with miR-1 and AR levels. These data suggest that the loss of KLF4 expression is one mechanistic link between aggressive prostate cancer progression and low canonical AR output through miR-1 inactivation.
Near-field surface plasmons on quasicrystal metasurfaces
Authors: Yang Quanlong , Zhang Xueqian , Li Shaoxian , Xu Quan , Singh Ranjan , Liu Yongmin , Li Yanfeng , Kruk Sergey S. , Gu Jianqiang , Han Jiaguang , Zhang Weili 
Published: December 2016

AbstractExcitation and manipulation of surface plasmons (SPs) are essential in developing cutting-edge plasmonic devices for medical diagnostics, biochemical spectroscopy and communications. The most common approach involves designing an array of periodic slits or grating apertures that enables coupling of the incident light to the SP modes. In recent years, plasmonic resonances, including extraordinary optical transmission through periodic arrays, quasicrystals and random aperture arrays, have been investigated in the free space. However, most of the studies have been limited to the far field detection of the transmission resonance. Here, we perform near-field measurements of the SPs on quasicrystal metasurfaces. We discover that the reciprocal vector determines the propagation modes of the SPs in the quasicrystal lattice which can be well explained by the quasi-momentum conservation rule. Our findings demonstrate vast potential in developing plasmonic metasurfaces with unique device functionalities that are controlled by the propagation modes of the SPs in quasicrystals.
Interferometric analysis of laser-driven cylindrically focusing shock waves in a thin liquid layer
Authors: Veysset David , Мaznev Alexei A. , Pezeril Thomas , Kooi Steven , Nelson Keith A. 
Published: December 2016

AbstractShock waves in condensed matter are of great importance for many areas of science and technology ranging from inertially confined fusion to planetary science and medicine. In laboratory studies of shock waves, there is a need in developing diagnostic techniques capable of measuring parameters of materials under shock with high spatial resolution. Here, time-resolved interferometric imaging is used to study laser-driven focusing shock waves in a thin liquid layer in an all-optical experiment. Shock waves are generated in a 10 µm-thick layer of water by focusing intense picosecond laser pulses into a ring of 95 µm radius. Using a Mach-Zehnder interferometer and time-delayed femtosecond laser pulses, we obtain a series of images tracing the shock wave as it converges at the center of the ring before reemerging as a diverging shock, resulting in the formation of a cavitation bubble. Through quantitative analysis of the interferograms, density profiles of shocked samples are extracted. The experimental geometry used in our study opens prospects for spatially resolved spectroscopic studies of materials under shock compression.
Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
Authors: Cao Xiaowei , Liu Jie , Chen Jian , Zheng Guo , Kuntner Matjaž , Agnarsson Ingi 
Published: December 2016

AbstractThe taxonomic impediment is characterized by dwindling classical taxonomic expertise, and slow pace of revisionary work, thus more rapid taxonomic assessments are needed. Here we pair rapid DNA barcoding methods with swift assessment of morphology in an effort to gauge diversity, establish species limits, and rapidly disseminate taxonomic information prior to completion of formal taxonomic revisions. We focus on a poorly studied, but diverse spider genus,Pseudopoda,from East Asia. We augmented the standard barcoding locus (COI) with nuclear DNA sequence data (ITS2) and analyzed congruence among datasets and species delimitation methods for a total of 572 individuals representing 23 described species and many potentially new species. Our results suggest that a combination of CO1 + ITS2 fragments identify and diagnose species better than the mitochondrial barcodes alone, and that certain tree based methods yield considerably higher diversity estimates than the distance-based approaches and morphology. Combined, through an extensive field survey, we detect a twofold increase in species diversity in the surveyed area, at 42–45, with most species representing short range endemics. Our study demonstrates the power of biodiversity assessments and swift dissemination of taxonomic data through rapid inventory, and through a combination of morphological and multi-locus DNA barcoding diagnoses of diverse arthropod lineages.
The Safety Limits Of An Extended Fast: Lessons from a Non-Model Organism
Authors: Bertile Fabrice , Fouillen Laetitia , Wasselin Thierry , Maes Pauline , Le Maho Yvon , Van Dorsselaer Alain , Raclot Thierry 
Published: December 2016

AbstractWhile safety of fasting therapy is debated in humans, extended fasting occurs routinely and safely in wild animals. To do so, food deprived animals like breeding penguins anticipate the critical limit of fasting by resuming feeding. To date, however, no molecular indices of the physiological state that links spontaneous refeeding behaviour with fasting limits had been identified. Blood proteomics and physiological data reveal here that fasting-induced body protein depletion is not unsafe “per se”. Indeed, incubating penguins only abandon their chick/egg to refeed when this state is associated with metabolic defects in glucose homeostasis/fatty acid utilization, insulin production and action, and possible renal dysfunctions. Our data illustrate how the field investigation of “exotic” models can be a unique source of information, with possible biomedical interest.
APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica
Authors: Zhang Mengchen , Yao Feng , Luan Hong , Zhao Wei , Jing Ting , Zhang Shuang , Hou Lin , Zou Xiangyang 
Published: December 2016

AbstractAnaphase Promoting Complex or Cyclosome (APC/C) is a representative E3 ubiquitin ligase, triggering the transition of metaphase to anaphase by regulating degradation and ensures the exit from mitosis. Cell division cycle 20 (CDC20) and Cell division cycle 20 related protein 1 (CDH1), as co-activators of APC/C, play significant roles in the spindle assembly checkpoint, guiding ubiquitin-mediated degradation, together with CDC23. During the embryonic development of the brine shrimp, Artemia sinica, CDC20, CDH1 and CDC23 participate in cell cycle regulation, but the specific mechanisms of their activities remain unknown. Herein, the full-length cDNAs of cdc20 and cdc23 from A. sinica were cloned. Real-time PCR analyzed the expression levels of As-cdc20 and As-cdc23. The locations of CDH1, CDC20 and CDC23 showed no tissue or organ specificity. Furthermore, western blotting showed that the levels of As-CDC20, securin, cyclin B, CDK1, CDH1, CDC14B, CDC23 and geminin proteins conformed to their complicated degradation relationships during different embryo stages. Our research revealed that As-CDC20, As-CDH1 and APC mediate the mitotic progression, downstream proteins degradation and cellular differentiation in the process of embryonic development in A. sinica.
Implications for management and conservation of the population genetic structure of the wedge clam Donax trunculus across two biogeographic boundaries
Authors: Marie Amandine D. , Lejeusne Christophe , Karapatsiou Evgenia , Cuesta José A. , Drake Pilar , Macpherson Enrique , Bernatchez Louis , Rico Ciro 
Published: December 2016

AbstractIn a resource management perspective, the understanding of the relative influence of the physical factors on species connectivity remains a major challenge and is also of great ecological and conservation biology interest. Despite the overfishing threat on the wedge clam Donax trunculus in Europe, relatively little information is known about its population genetic structure and connectivity and their consequences on conservation policies. We employed 16 microsatellite loci to characterise the genetic diversity and population structure of D. trunculus. A total of 514 samples from seven different localities along the Atlantic-Mediterranean transition, from the Atlantic (Gulf of Cádiz) to the north-western Mediterranean were genotyped. The analysis of the population genetic structure displayed a clear distinction along the Atlantic-Mediterranean transition with different clusters in the Atlantic Ocean, the Alboran Sea and the northwestern Mediterranean. Consequently, we recommend that these three areas should be considered as different management units. We showed that all populations seem to be at high long-term risk of extinction with the exception of the protected Doñana National Park population which still seems to have evolutionary potential. Therefore, our results emphasized the necessity of protection of this economic resource and the validity of molecular tools to evaluate the population dynamics.
Possible Source Populations of the White-backed Planthopper in the Greater Mekong Subregion Revealed by Mitochondrial DNA Analysis
Authors: Li Xiang-yong , Chu Dong , Yin Yan-qiong , Zhao Xue-qing , Chen Ai-dong , Khay Sathya , Douangboupha Bounneuang , Kyaw Mu Mu , Kongchuensin Manita , Ngo Vien Vinh , Nguyen Chung Huy 
Published: December 2016

AbstractThe white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is a serious pest of rice in Asia. However, little is known regarding the migration of this pest insect from the Greater Mekong Subregion (GMS) including Cambodia, Laos, Myanmar (Burma), Thailand, and Vietnam, into China’s Yunnan Province. To determine the migration patterns of S. furcifera in the GMS and putative secondary immigration inside China’s Yunnan Province, we investigated the population genetic diversity, genetic structure, and gene flow of 42 S. furcifera populations across the six countries in the GMS by intensive sampling using mitochondrial genes. Our study revealed the potential emigration of S. furcifera from the GMS consists primarily of three major sources: 1) the S. furcifera from Laos and Vietnam migrate into south and southeast Yunnan, where they proceed to further migrate into northeast and central Yunnan; 2) the S. furcifera from Myanmar migrate into west Yunnan, and/or central Yunnan, and/or northeast Yunnan; 3) the S. furcifera from Cambodia migrate into southwest Yunnan, where the populations can migrate further into central Yunnan. The new data will not only be helpful in predicting population dynamics of the planthopper, but will also aid in regional control programs for this economically important pest insect.