bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short‐term and long‐term effects of a minimally invasive transilial vertebral blocking procedure on the lumbosacral morphometry in dogs measured by computed tomography
Authors: Müller Friedrich , Schenk Henning C. , Forterre Franck 
Published: March 2017

AbstractObjectiveTo determine the effects of a minimally invasive transilial vertebral (MTV) blocking procedure on the computed tomographic (CT) appearance of the lumbosacral (L7/S1) junction of dogs with degenerative lumbosacral stenosis (DLSS).Study designProspective study.Animals59 client‐owned dogs with DLSS.MethodsLumbosacral CT images were acquired with hyperextended pelvic limbs before and after MTV in all dogs. Clinical follow‐up was obtained after 1 year, including a neurologic status classified in 4 grades, and if possible, CT. Morphometric measurements (Mean ± SEM) including foraminal area, endplate distance at L7/S1 and LS angle were obtained on sets of reformatted parasagittal and sagittal CT images.ResultsThe mean foraminal area (ForL) increased from 32.5 ± 1.7 mm2 to 59.7 ± 1.9 mm2 on the left and from 31.1 ± 1.4 mm2 to 59.1 ± 2.0 mm2 on the right (ForR) side after MTV. The mean endplate distance (EDmd) between L7/S1 increased from 3.7 ± 0.1 mm to 6.0 ± 0.1 mm, and mean lumbosacral angle (LSa) from 148.0 ± 1.1° to 170.0 ± 1.1° after MTV. CT measurements were available 1 year postoperatively in 12 cases: ForL: 41.2 ± 3.1 mm2; ForR: 37.9 ± 3.1 mm2; EDmd: 4.3 ± 0.4 mm, and LSa 157.6 ± 2.1° (values are mean and standard error of mean =  SEM). All 39 dogs with long‐term follow‐up improved by at least 1 neurologic grade, 9/39 improving by 3 grades, 15/39 by 2 grades, and 15/39 by 1 grade.ConclusionsMTV results in clinical improvement and morphometric enlargement of the foraminal area in dogs with variable degrees of foraminal stenosis.Clinical relevanceMTV may be a valuable minimally invasive option for treatment of dogs with DLSS.
Non‐muscle (NM) myosin heavy chain phosphorylation regulates the formation of NM myosin filaments, adhesome assembly and smooth muscle contraction
Authors: Zhang Wenwu , Gunst Susan J. 
Published: March 2017

Key points
Non‐muscle (NM) and smooth muscle (SM) myosin II are both expressed in smooth muscle tissues, however the role of NM myosin in SM contraction is unknown.
Contractile stimulation of tracheal smooth muscle tissues stimulates phosphorylation of the NM myosin heavy chain on Ser1943 and causes NM myosin filament assembly at the SM cell cortex.
Expression of a non‐phosphorylatable NM myosin mutant, NM myosin S1943A, in SM tissues inhibits ACh‐induced NM myosin filament assembly and SM contraction, and also inhibits the assembly of membrane adhesome complexes during contractile stimulation.
NM myosin regulatory light chain (RLC) phosphorylation but not SM myosin RLC phosphorylation is regulated by RhoA GTPase during ACh stimulation, and NM RLC phosphorylation is required for NM myosin filament assembly and SM contraction.
NM myosin II plays a critical role in airway SM contraction that is independent and distinct from the function of SM myosin.
AbstractThe molecular function of non‐muscle (NM) isoforms of myosin II in smooth muscle (SM) tissues and their possible role in contraction are largely unknown. We evaluated the function of NM myosin during contractile stimulation of canine tracheal SM tissues. Stimulation with ACh caused NM myosin filament assembly, as assessed by a Triton solubility assay and a proximity ligation assay aiming to measure interactions between NM myosin monomers. ACh stimulated the phosphorylation of NM myosin heavy chain on Ser1943 in tracheal SM tissues, which can regulate NM myosin IIA filament assembly in vitro. Expression of the non‐phosphorylatable mutant NM myosin S1943A in SM tissues inhibited ACh‐induced endogenous NM myosin Ser1943 phosphorylation, NM myosin filament formation, the assembly of membrane adhesome complexes and tension development. The NM myosin cross‐bridge cycling inhibitor blebbistatin suppressed adhesome complex assembly and SM contraction without inhibiting NM myosin Ser1943 phosphorylation or NM myosin filament assembly. RhoA inactivation selectively inhibited phosphorylation of the NM myosin regulatory light chain (RLC), NM myosin filament assembly and contraction, although it did not inhibit SM RLC phosphorylation. We conclude that the assembly and activation of NM myosin II is regulated during contractile stimulation of airway SM tissues by RhoA‐mediated NM myosin RLC phosphorylation and by NM myosin heavy chain Ser1943 phosphorylation. NM myosin II actomyosin cross‐bridge cycling regulates the assembly of membrane adhesome complexes that mediate the cytoskeletal processes required for tension generation. NM myosin II plays a critical role in airway SM contraction that is independent and distinct from the function of SM myosin.
Draft Genome Sequence of a Multidrug-Resistant Strain of Enterococcus faecalis, PM01, Isolated from the Nest of an American Bushtit,
Psaltriparius minimus
Authors: Esani Saika , Constable John V. H. , Van Laar Tricia A. 
Published: March 2017

ABSTRACT

Pathogenic microorganisms associated with avian nests may detrimentally impact parental health and nest success for the nest primary users, potentially neighboring avian or terrestrial species, including humans. Here, we report the genome sequence of
Enterococcus faecalis
strain PM01, isolated from a failed nest of American bushtits,
Psaltriparius minimus
.
Complete Genome and Methylome Sequences of Salmonella enterica subsp.
enterica
Serovars Typhimurium, Saintpaul, and Stanleyville from the SARA/SARB Collection
Authors: Yao Kuan , Roberts Richard J. , Allard Marc W. , Hoffmann Maria 
Published: March 2017

ABSTRACT

In this announcement, we report the complete genome and methylome sequences of three
Salmonella enterica
strains from the SARA and SARB collection:
S. enterica
subsp.
enterica
serovar Typhimurium (SARA13),
S. enterica
subsp.
enterica
serovar Saintpaul (SARA26), and
S. enterica
subsp.
enterica
serovar Stanleyville (SARB61).
Draft Genome Sequences of Escherichia coli Strains Isolated at Calving from the Uterus, Vagina, Vulva, and Rectoanal Junction of a Dairy Cow That Later Developed Metritis
Authors: Jeon Soo Jin , Cunha Federico , Ginn Amber , Jeong KwangCheol Casey , Galvão Klibs N. 
Published: March 2017

ABSTRACT

Escherichia coli
is involved in the pathogenicity of metritis in cows. We report here the genome sequences of
E. coli
strains isolated at calving from the uterus, vagina, vulva, and rectoanal junction of a dairy cow that later developed metritis. The genomic similarities will give an insight into phylogenetic relationships among strains.
Genome Sequence of Cluster W Mycobacteriophage Taptic
Authors: Mageeney Catherine M. , Seier Emily R. , Esposito Elise C. , Graham Lee H. , Heckman Emily L. , Hipwell Chelsea M. , Kelliher Allison B. , Lando Nicole A. , Morales Patricia Y. , Russell Daniel A. , Tsaousis Barbara E. , Kenna Margaret A. , Ware Vassie C. 
Published: March 2017

ABSTRACT

The Taptic genome is the first to be annotated from the W cluster of mycobacteriophages infecting
Mycobacterium smegmatis
mc
2
155. All 92 predicted open reading frames (ORFs) and a single tRNA specifying glycine (tRNA-gly) are transcribed rightward. Many functionally uncharacterized ORFs appear to be W cluster specific, as nucleotide similarity is shared only with other W cluster genomes.
High-Quality Genome Sequence of an Escherichia coli O157 Strain Carrying an
mcr-1
Resistance Gene Isolated from a Patient in the United States
Authors: Lindsey Rebecca L. , Batra Dhwani , Rowe Lori , Loparev Vladimir N. , Stripling Devon , Garcia-Toledo Lisley , Knipe Kristen , Juieng Phalasy , Sheth Mili , Martin Haley , Laufer Halpin Alison 
Published: March 2017

ABSTRACT

Enterobacteriaceae
carrying plasmid-mediated colistin resistance have been found around the world. We report here the high-quality whole-genome sequence of an
Escherichia coli
O157:H48 isolate (2016C-3936C1) from Connecticut that carried the
mcr-1
resistance gene on an IncX4-type plasmid.
Genomic Characterization of Three Melon Necrotic Spot Viruses Detected in Human Stool Specimens
Authors: Marine Rachel , Castro Christina , Magaña Laura , Ng Terry Fei Fan , Aswath Kshama , Collins Nikail , Park Geun Woo , Vinjé Jan , Oberste M. Steven 
Published: March 2017

ABSTRACT
The complete coding sequences of three melon necrotic spot viruses (MNSVs) were obtained from viral metagenomics of stool samples from patients with acute gastroenteritis. These genomes were most similar to Spanish strains sequenced in 2003 and a novel MNSV watermelon strain in 2014.
Paradoxical Reactions and the Immune Reconstitution Inflammatory Syndrome
Authors: Church L. W. Preston , Chopra Amit , Judson Marc A. 
Published: March 2017

ABSTRACT
In HIV-infected individuals, paradoxical reactions after the initiation of antiretroviral therapy (ART) are associated with a variety of underlying infections and have been called the immune reconstitution inflammatory syndrome (IRIS). In cases of IRIS associated with tuberculosis (TB), two distinct patterns of disease are recognized: (i) the progression of subclinical TB to clinical disease after the initiation of ART, referred to as unmasking, and (ii) the progression or appearance of new clinical and/or radiographic disease in patients with previously recognized TB after the initiation of ART, the classic or “paradoxical” TB-IRIS. IRIS can potentially occur in all granulomatous diseases, not just infectious ones. All granulomatous diseases are thought to result from interplay of inflammatory cells and mediators. One of the inflammatory cells thought to be integral to the development of the granuloma is the CD4 T lymphocyte. Therefore, HIV-infected patients with noninfectious granulomatous diseases such as sarcoidosis may also develop IRIS reactions. Here, we describe IRIS in HIV-infected patients with TB and sarcoidosis and review the basic clinical and immunological aspects of these phenomena.
Systematic review of systematic reviews of non-pharmacological interventions to treat behavioural disturbances in older patients with dementia. The SENATOR-OnTop series
Authors: Abraha Iosief , Rimland Joseph M , Trotta Fabiana Mirella , Dell'Aquila Giuseppina , Cruz-Jentoft Alfonso , Petrovic Mirko , Gudmundsson Adalsteinn , Soiza Roy , O'Mahony Denis , Guaita Antonio , Cherubini Antonio 
Published: March 2017

ObjectiveTo provide an overview of non-pharmacological interventions for behavioural and psychological symptoms in dementia (BPSD).DesignSystematic overview of reviews.Data sourcesPubMed, EMBASE, Cochrane Database of Systematic Reviews, CINAHL and PsycINFO (2009–March 2015).Eligibility criteriaSystematic reviews (SRs) that included at least one comparative study evaluating any non-pharmacological intervention, to treat BPSD.Data extractionEligible studies were selected and data extracted independently by 2 reviewers.The AMSTAR checklist was used to assess the quality of the SRs.Data analysisExtracted data were synthesised using a narrative approach.Results38 SRs and 142 primary studies were identified, comprising the following categories of non-pharmacological interventions: (1) sensory stimulation interventions (12 SRs, 27 primary studies) that encompassed: acupressure, aromatherapy, massage/touch therapy, light therapy and sensory garden; (2) cognitive/emotion-oriented interventions (33 SRs; 70 primary studies) that included cognitive stimulation, music/dance therapy, dance therapy, snoezelen, transcutaneous electrical nerve stimulation, reminiscence therapy, validation therapy, simulated presence therapy; (3) behaviour management techniques (6 SRs; 32 primary studies) and (4) other therapies (5 SRs, 12 primary studies) comprising exercise therapy, animal-assisted therapy, special care unit and dining room environment-based interventions. Music therapy was effective in reducing agitation (SMD, −0.49; 95% CI −0.82 to −0.17; p=0.003), and anxiety (SMD, −0.64; 95% CI −1.05 to −0.24; p=0.002). Home-based behavioural management techniques, caregiver-based interventions or staff training in communication skills, person-centred care or dementia care mapping with supervision during implementation were found to be effective for symptomatic and severe agitation.ConclusionsA large number of non-pharmacological interventions for BPSD were identified. The majority of the studies had great variation in how the same type of intervention was defined and applied, the follow-up duration, the type of outcome measured, usually with modest sample size. Overall, music therapy and behavioural management techniques were effective for reducing BPSD.
Innovative approach for self-management and social welfare of children with cystic fibrosis in Europe: development, validation and implementation of an mHealth tool (MyCyFAPP)
Authors: Calvo-Lerma Joaquim , Martinez-Jimenez Celia P , Lázaro-Ramos Juan-Pablo , Andrés Ana , Crespo-Escobar Paula , Stav Erlend , Schauber Cornelia , Pannese Lucia , Hulst Jessie M , Suárez Lucrecia , Colombo Carla , Barreto Celeste , de Boeck Kris , Ribes-Koninckx Carmen 
Published: March 2017

IntroductionFor the optimal management of children with cystic fibrosis, there are currently no efficient tools for the precise adjustment of pancreatic enzyme replacement therapy, either for advice on appropriate dietary intake or for achieving an optimal nutrition status. Therefore, we aim to develop a mobile application that ensures a successful nutritional therapy in children with cystic fibrosis.Methods and analysisA multidisciplinary team of 12 partners coordinate their efforts in 9 work packages that cover the entire so-called ‘from laboratory to market’ approach by means of an original and innovative co-design process. A cohort of 200 patients with cystic fibrosis aged 1–17 years are enrolled. We will develop an innovative, clinically tested mobile health application for patients and health professionals involved in cystic fibrosis management. The mobile application integrates the research knowledge and innovative tools for maximising self-management with the aim of leading to a better nutritional status, quality of life and disease prognosis. Bringing together different and complementary areas of knowledge is fundamental for tackling complex challenges in disease treatment, such as optimal nutrition and pancreatic enzyme replacement therapy in cystic fibrosis. Patients are expected to benefit the most from the outcomes of this innovative project.Ethics and disseminationThe project is approved by the Ethics Committee of the coordinating organisation, Hospital Universitari La Fe (Ref: 2014/0484). Scientific findings will be disseminated via journals and conferences addressed to clinicians, food scientists, information and communications technology experts and patients. The specific dissemination working group within the project will address the wide audience communication through the website (http://www.mycyfapp.eu), the social networks and the newsletter.
Effects of mechanism of injury and patient age on outcomes in geriatric rib fracture patients
Authors: Shi Helen H , Esquivel Micaela , Staudenmayer Kristan L , Spain David A 
Published: March 2017

BackgroundPatients older than 65 years have 2–5 times higher mortality if they sustain ≥2 rib fractures compared to younger adults. As a result, our level I trauma center guidelines suggest that older adults with rib fractures be admitted to the intensive care unit for the first 24 hours. In this study, we evaluated the outcomes associated with these guidelines.MethodsWe retrospectively reviewed all patients aged ≥65 years in our Trauma Registry who sustained rib fractures from January 2008 to March 2015. Data included demographics, comorbidities, injuries, length of intensive care and hospital stay (LOS), ventilator days, analgesic used, morbidity, mortality, and disposition.Results97 patients aged ≥65 years with at least one rib fracture and an Abbreviated Injury Score of ≤2 for other regions were admitted. Falls caused 58% of the injuries, while motor vehicle collisions (MVC) accounted for 33%. Overall mortality was 4%. Patients who fell had a median hospital LOS that was 0.5 to 1 day longer than in those who suffered other mechanisms of injury or were involved in an MVC respectively. Patients aged ≥70 years had a median LOS of 4 days, twice that of those aged 65 to 69 years. Of the 87 patients with more than one rib fracture, 59 (68%) were not admitted directly to the intensive care unit (ICU) from the emergency department as recommended by our guidelines. 6 of these 59 patients (9%) were later transferred to the ICU and 2 of these patients expired.ConclusionsAlthough overall compliance with the geriatric rib fracture guideline was low, both mortality and hospital LOS were low in this group. This suggests that the guideline could be modified to reduce ICU resource usage without compromising patient outcomes.Level of evidenceLevel III, retrospective cohort study.
Warped cones over profinite completions
Authors: Sawicki Damian 
Published: March 2017

We construct metric spaces that do not have property A yet are coarsely embeddable into the Hilbert space. Our examples are so-called warped cones, which were introduced by J. Roe to serve as examples of spaces non-embeddable into a Hilbert space and with or without property A. The construction provides the first examples of warped cones combining coarse embeddability and lack of property A. We also construct warped cones over manifolds with isometrically embedded expanders and generalise Roe’s criteria for the lack of property A or coarse embeddability of a warped cone. Along the way, it is proven that property A of the warped cone over a profinite completion is equivalent to amenability of the group. In the Appendix we solve a problem of Nowak regarding his examples of spaces with similar properties.
On the associativity of gluing
Authors: Qin Lizhen 
Published: March 2017

This paper studies the associativity of gluing of trajectories in Morse theory. We show that the associativity of gluing follows from the existence of compatible manifold with face structures on the compactified moduli spaces. Using our previous work, we obtain the associativity of gluing in certain cases. In particular, associativity holds when the ambient manifold is compact and the vector field is Morse–Smale.
Loss of VEGFB and its signaling in the diabetic heart is associated with increased cell death signaling
Authors: Lal Nathaniel , Chiu Amy Pei-Ling , Wang Fulong , Zhang Dahai , Jia Jocelyn , Wan Andrea , Vlodavsky Israel , Hussein Bahira , Rodrigues Brian 
Published: March 2017

Vascular endothelial growth factor B (VEGFB) is highly expressed in metabolically active tissues, such as the heart and skeletal muscle, suggesting a function in maintaining oxidative metabolic and contractile function in these tissues. Multiple models of heart failure have indicated a significant drop in VEGFB. However, whether there is a role for decreased VEGFB in diabetic cardiomyopathy is currently unknown. Of the VEGFB located in cardiomyocytes, there is a substantial and readily releasable pool localized on the cell surface. The immediate response to high glucose and the secretion of endothelial heparanase is the release of this surface-bound VEGFB, which triggers signaling pathways and gene expression to influence endothelial cell (autocrine action) and cardiomyocyte (paracrine effects) survival. Under conditions of hyperglycemia, when VEGFB production is impaired, a robust increase in vascular endothelial growth factor receptor (VEGFR)-1 expression ensues as a possible mechanism to enhance or maintain VEGFB signaling. However, even with an increase in VEGFR1 after diabetes, cardiomyocytes are unable to respond to VEGFB. In addition to the loss of VEGFB production and signaling, evaluation of latent heparanase, the protein responsible for VEGFB release, also showed a significant decline in expression in whole hearts from animals with chronic or acute diabetes. Defects in these numerous VEGFB pathways were associated with an increased cell death signature in our models of diabetes. Through this bidirectional interaction between endothelial cells (which secrete heparanase) and cardiomyocytes (which release VEGFB), this growth factor could provide the diabetic heart protection against cell death and may be a critical tool to delay or prevent cardiomyopathy. NEW & NOTEWORTHY We discovered a bidirectional interaction between endothelial cells (which secrete heparanase) and cardiomyocytes [which release vascular endothelial growth factor B (VEGFB)]. VEGFB promoted cell survival through ERK and cell death gene expression. Loss of VEGFB and its downstream signaling is an early event following hyperglycemia, is sustained with disease progression, and could explain diabetic cardiomyopathy.
FADD, Caspase-3, and Caspase-8 and Incidence of Coronary Events
Authors: Xue Ling , Borné Yan , Mattisson Ingrid Yao , Wigren Maria , Melander Olle , Ohro-Melander Marju , Bengtsson Eva , Fredrikson Gunilla Nordin , Nilsson Jan , Engström Gunnar 
Published: March 2017

Objective—
To investigate the relationship between 3 markers of apoptosis, that is, FADD (Fas-associated death domain–containing protein), caspase-3, and caspase-8, and incidence of coronary events (CEs) in a population-based cohort study.

Approach and Results—

In vitro experiments were performed to assess the response of the apoptotic biomarkers after Fas stimulation of peripheral blood mononuclear cells. The experiments showed significantly increased releases of FADD, caspase-3, and caspase-8 after Fas stimulation. The relationship between FADD, caspase-3, and caspase-8, respectively, and incidence of CEs was studied in 4284 subjects from the population-based Malmö Diet and Cancer Study. Cox’ proportional hazards regression was used to examine the association between the apoptotic biomarkers and incidence of CE over a mean follow-up of 19 years. A total of 381 individuals had CE during the follow-up. High FADD at baseline was significantly associated with incident CE. In the highest compared with the lowest quartile of FADD, the risk factor adjusted hazards ratio for CE was 1.82 (95% confidence interval, 1.35–2.46;
P
for trend <0.001). A significant association was also found between caspase-8 and CE; the hazards ratio (Q4 versus Q1) was 1.90 (95% confidence interval, 1.39–2.60;
P
for trend <0.001) after adjustment for risk factors. No association was found between caspase-3 and CEs.

Conclusions—
High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of CE in subjects from the general population. The in vitro experiments support the view that these biomarkers could reflect activation of the extrinsic apoptotic pathway.
Participant-perceived outcomes of merging yoga and occupational therapy: Self-management intervention for people post stroke
Authors: Atler Karen E , Van Puymbroeck Marieke , Portz Jennifer D , Schmid Arlene A 
Published: March 2017

Introduction There is a need for development and validation of post-stroke fall-prevention programs. This qualitative study provides an inside look into participant-perceived outcomes of a new and innovative self-management intervention: Merging Yoga and Occupational Therapy. Method A qualitative approach was employed. Thirteen participants, who were more than 6 months post stroke and self-reported falling or a fear of falling, engaged in focus groups and individual interviews following participation in the intervention twice weekly for 8 weeks. Data from focus groups and individual interviews were analyzed using an iterative, inductive approach. Findings Five outcomes emerged from the line-by-line analysis: (1) improved abilities and capacities, (2) gained new knowledge, (3) enhanced engagement in activities, (4) improved relaxation, and (5) increased confidence and inspiration. Conclusion Participants experienced positive outcomes from engagement in a community-based multimodal fall-prevention self-management program for people post stroke.