bluuebunny/crossref_metadata_2025_split huggingface dataset:
Botulinum Toxin Injection for Internal Rotation Contractures in Brachial Plexus Birth Palsy. A Minimum 5-Year Prospective Observational Study
Authors: Duijnisveld Bouke J. , van Wijlen-Hempel Marie S. , Hogendoorn Simone , de Boer Kees S. , Malessy Martijn J.A. , Keurentjes J. Christiaan , Nagels Jochem , Nelissen Rob G.H.H. 
Published: June 2016

Background:
Brachial plexus birth palsy is frequently associated with internal rotation contractures of the shoulder as a result of muscle imbalance. The purpose of this study is to assess the effect of botulinum toxin A (BTX-A) injection in the subscapular (SC) muscle on external rotation and the need for tendon transfer for external rotation of the shoulder.

Methods:
A prospective comparative study was performed including 15 consecutive patients treated with BTX-A and a historic control group of 67 patients with mean age 30 months (SD 10). The BTX-A injection (2 IU/kg body weight) was performed immediately following MRI under general anesthesia in the SC muscle. Passive external rotation, the need for tendon transfer surgery, glenohumeral deformity, and muscle degeneration were evaluated. The hazard ratio for no relapse of internal rotation contracture after BTX-A injection compared with no BTX-A injection was calculated.

Results:
In the BTX-A group, the passive external rotation in adduction increased from −1 degree (95% CI, −10 to 8) to 32 degrees (95% CI, 17-46) at 3 months and 6 patients were indicated for surgery compared with a decline from −2 degrees (95% CI, −7 to 3) to −11 degrees (95% CI, −17 to −6) in the control group with 66 indications for surgery. At 5 years of follow-up, 10 patients in the BTX-A group were indicated for surgery with a hazard ratio of 4.0 (95% CI, 1.9 to 8.4).

Conclusions:
BTX-A injection in the SC muscle of brachial plexus birth palsy patients can reduce internal rotation contractures and subsequently the need for tendon transfer surgery. At 5 years of follow-up a relapse was seen in 67% of the patients treated with BTX-A. Because at MRI less SC degeneration was found in the good responders on BTX-A treatment, this group seems to be the best target group. Further research is needed on patient selection for BTX-A injection including glenohumeral deformity, SC degeneration, as well as doses of BTX-A to be used.

Level of Evidence:
Level II—prospective comparative study.
Patient-Induced Reaction Forces and Moments Are Influenced by Variations in Spinal Manipulative Technique
Authors: D’Angelo Kevin , Triano John J. , Kawchuk Gregory N. , Howarth Samuel J. 
Published: June 2016

Study Design.
An in vivo biomechanical study.

Objective.
The aim of the present study was to quantify and compare the reaction loads for two spinal manipulation therapy (SMT) procedures commonly used for low back pain using a biomechanical computer model.

Summary of Background Data.
Contemporary computer-driven rigid linked-segment models (LSMs) have made it feasible to analyze low back kinetics and kinematics during various activities including SMT procedures. Currently, a comprehensive biomechanical model analyzing actual differences in loading effects between different SMT procedures is lacking.

Methods.
Twenty-four healthy/asymptomatic participants received a total of six SMT applications, representing all combinations of two similar SMT procedures within three patient hip flexion angles. All contact forces, patient torso kinematics, and inertial properties were entered into a dynamic three-dimensional LSM to calculate lumbar reaction forces and moments. Peak net applied force along with the maximums, minimums, and ranges for each component of the three-dimensional reaction force and moment vectors during each SMT procedure was analyzed.

Results.
One specific SMT technique (lumbar spinous pull) produced greater maximum anterior-posterior reaction force and both lateral bending and axial twisting reaction moments compared to the other technique (lumbar push procedure [all P ≤ 0.034]). SMT trials without hip flexion had lower maximum medial-lateral reaction force and range compared to those with 45 and 90 degrees of hip flexion (all P ≤ 0.041). There were no interactions between procedure and hip angle for any of the dependent measurements.

Conclusion.
The technique used to apply SMT and the participant's initial hip orientation induced significantly different actions on the low back. These findings and future research can improve patient outcomes and safety by informing clinicians on how to best use SMT given specific types of low back pain.

Level of Evidence: 2
Perioperative Mortality in Nonelderly Adult Patients With Cancer
Authors: Amini Arya , Yeh Norman , Jones Bernard L. , Bedrick Edward , Vinogradskiy Yevgeniy , Rusthoven Chad G. , Amini Ava , Purcell William T. , Karam Sana D. , Kavanagh Brian D. , Guntupalli Saketh R. , Fisher Christine M. 
Published: June 2016

Objectives:
The purpose of this study was to evaluate whether insurance status predicts for perioperative mortality (death within 30 d of cancer-directed surgery) for the 20 most common surgically treated cancers.

Methods:
The SEER database was examined for the 20 most common surgically resected cancers and included nonelderly adults, aged 18 to 64 years. The database was queried from 2007 to 2011, with a total of 506,722 patients included in the analysis.

Results:
Insurance status for all patients were the following: non-Medicaid insurance (83%), any Medicaid (10%), uninsured (4%), and unknown (3%). In univariate analyses, predictors for perioperative mortality included insurance status (P<0.001), age (P=0.015), race (P<0.001), marital status (P<0.001), residence (P=0.002), percent of county below the federal poverty level (P<0.001), and median county-level income (P<0.001). Perioperative mortality was also associated with advanced disease (P<0.001). Under multivariate analysis, patients with either Medicaid (Cochran-Mantel-Haenszel odds ratio [CMH OR], 1.21; 95% confidence interval [CI], 1.14-1.29; P<0.001) or uninsured status (CMH OR, 1.56; 95% CI, 1.44-1.70; P<0.001) were more likely to die within 30 days of surgery compared with patients with non-Medicaid insurance. When comparing Medicaid with the uninsured, Medicaid patients had significantly lower rates of perioperative mortality when compared with the uninsured (CMH OR, 0.80; 95% CI, 0.73-0.89, P<0.001).

Conclusions and Relevance:
In the largest reported analysis of perioperative mortality evaluating the 20 most common surgically treated malignancies, patients with Medicaid coverage or without health insurance were more likely to die within 30 days of surgery, with the uninsured having the worst outcomes.
Peri- and postoperative cognitive and consecutive functional problems of elderly patients
Authors: Müller Anika , Lachmann Gunnar , Wolf Alissa , Mörgeli Rudolf , Weiss Björn , Spies Claudia 
Published: June 2016

Purpose of review
From an elderly patient's perspective, acute and chronic cognitive disturbances are among the most harmful complications that can occur following surgery. For elderly patients, these complications often mean the end of an independent life. This article focuses on this serious aspect, which is increasingly prevalent in our aging society. Cognitive disturbances are associated with severe outcome impairments and increased mortality. This article aims to provide a current overview regarding the diagnosis, pathophysiology, prevention, and treatment of this severe social problem.

Recent findings
The current knowledge of risk factors, diagnosis, prevention, and treatment of postoperative delirium and postoperative cognitive dysfunction should help to raise awareness and improve the outcome of delirious patients, particularly in the elderly population.

Summary
Especially in elderly patients, postoperative delirium constitutes a common, severe complication. Early diagnosis and supportive treatment are essential to improve outcome. To date, no pharmacological treatment strategy was effective, so that further research about the underlying pathophysiology and the development of treatment strategies are urgently required.
Interventions that Enhance Breastfeeding Initiation, Duration, and Exclusivity
Authors: Wood Natsuko K. , Woods Nancy F. , Blackburn Susan T. , Sanders Elizabeth A. 
Published: June 2016

Abstract

Objective:
The purpose of this review was to evaluate breastfeeding interventions trialed to date and recommend directions for future needs in breastfeeding research.

Methods:
A literature review was conducted using PubMed, CINAHL Plus, and PsycINFO databases to identify studies that evaluated efficacy or effectiveness of breastfeeding interventions on breastfeeding initiation, duration, or exclusivity as a primary, secondary, or tertiary outcome. Combinations of search terms included breastfeeding, feeding behavior, prenatal/patient education, health promotion, social support, perinatal/prenatal/intrapartum/postnatal care, and postpartum period.

Results:
Six studies were included in this review, using PRISMA guidelines. Acquisition of knowledge and skills, emotional support by healthcare providers, and self-efficacy over maternal confidence in her ability to breastfeed were factors the intervention studies relied on to affect breastfeeding practices. Although these factors were addressed in the studies, breastfeeding mothers had difficulty transferring what they gained from interventions into their real-life breastfeeding practices as evidenced by the highest drop-off rate of exclusive breastfeeding in the early postpartum.

Conclusions:
There were conceptual limitations to the reviewed studies: (1) lack of understanding of maternal perception of infant behavior and (2) perceived insufficient milk as a remaining primary reason for breastfeeding discontinuation. There were methodological limitations: (1) lack of theory-based interventions and (2) lack of intervention fidelity. Future studies involving breastfeeding should focus on the causes of the problems driven by theory-based interventions integrated with intervention fidelity.
Evolutionary dynamics of CRISPR gene drives
Authors: Noble Charleston , Olejarz Jason , Esvelt Kevin M. , Church George M. , Nowak Martin A. 
Published: June 2016

AbstractThe alteration of wild populations has been discussed as a solution to a number of humanity’s most pressing ecological and public health concerns. Enabled by the recent revolution in genome editing, CRISPR gene drives, selfish genetic elements which can spread through populations even if they confer no advantage to their host organism, are rapidly emerging as the most promising approach. But before real-world applications are considered, it is imperative to develop a clear understanding of the outcomes of drive release in nature. Toward this aim, we mathematically study the evolutionary dynamics of CRISPR gene drives. We demonstrate that the emergence of drive-resistant alleles presents a major challenge to previously reported constructs, and we show that an alternative design which selects against resistant alleles greatly improves evolutionary stability. We discuss all results in the context of CRISPR technology and provide insights which inform the engineering of practical gene drive systems.
Simulated forward and backward self motion, based on realistic parameters, causes strong motion induced blindness
Authors: Thomas Victoria , Davidson Matthew , Zakavi Parisa , Tsuchiya Naotsugu , van Boxtel Jeroen 
Published: June 2016

ABSTRACTMotion Induced Blindness (MIB) is a well-established visual phenomenon whereby highly salient targets disappear when viewed against a moving background mask. No research has yet explored whether contracting and expanding optic flow can also trigger target disappearance. We explored MIB using mask speeds corresponding to driving at 35, 50, 65 and 80 km/h in simulated forward (expansion) and backward (contraction) motion as well as 2-D radial movement, random, and static mask motion types. Participants (n= 18) viewed MIB targets against masks with different movement types, speed, and target locations. To understand the relationship between saccades, pupil response and perceptual disappearance, we ran two additional eye-tracking experiments (n= 19). Target disappearance increased significantly with faster mask speeds and upper visual field target presentation. Simulated optic flow and 2-D radial movement caused comparable disappearance, and all moving masks caused significantly more disappearance than a static mask. Saccades could not entirely account for differences between conditions, suggesting that self-motion optic flow does cause MIB in an artificial setting. Pupil analyses implied that MIB disappearance induced by optic flow is not subjectively salient, potentially explaining why MIB is not noticed during driving. Potential implications of MIB for driving safety and Head-Up-Display (HUD) technologies are discussed.
Integrative modeling of tumor DNA methylation identifies a role for metabolism
Authors: Mehrmohamadi Mahya , Mentch Lucas K. , Clark Andrew G. , Locasale Jason W. 
Published: June 2016

AbstractDNA methylation varies across genomic regions, tissues and individuals in a population. Altered DNA methylation is common in cancer and often considered an early event in tumorigenesis. However, the sources of heterogeneity of DNA methylation among tumors remain poorly defined. Here, we capitalize on the availability of multi-platform data on thousands of molecularly-and clinically-annotated human tumors to build integrative models that identify the determinants of DNA methylation. We quantify the relative contribution of clinical and molecular factors in explaining within-cancer (inter-individual) variability in DNA methylation. We show that the levels of a set of metabolic genes involved in the methionine cycle that are constituents of one-carbon metabolism are predictive of several features of DNA methylation status in tumors including the methylation of genes that are known to drive oncogenesis. Finally, we demonstrate that patients whose DNA methylation status can be predicted from the genes in one-carbon metabolism exhibited improved survival over cases where this regulation is disrupted. To our knowledge, this study is the first comprehensive analysis of the determinants of methylation and demonstrates the surprisingly large contribution of metabolism in explaining epigenetic variation among individual tumors of the same cancer type. Together, our results illustrate links between tumor metabolism and epigenetics and outline future clinical implications.
Topslam: Waddington Landscape Recovery for Single Cell Experiments
Authors: Zwiessele Max , Lawrence Neil D 
Published: June 2016

AbstractWe present an approach to estimating the nature of the Waddington (or epigenetic) landscape that underlies a population of individual cells. Through exploiting high resolution single cell transcription experiments we show that cells can be located on a landscape that reflects their differentiated nature.Our approach makes use of probabilistic non-linear dimensionality reduction that respects the topology of our estimated epigenetic landscape. In simulation studies and analyses of real data we show that the approach, known as topslam, outperforms previous attempts to understand the differentiation landscape.Hereby, the novelty of our approach lies in the correction of distancesbeforeextracting ordering information. This gives the advantage over other attempts, which have to correct for extracted time lines by post processing or additional data.
Genome-wide characterization of genetic and functional dysregulation in autism spectrum disorder
Authors: Krishnan Arjun , Zhang Ran , Yao Victoria , Theesfeld Chandra L. , Wong Aaron K. , Tadych Alicja , Volfovsky Natalia , Packer Alan , Lash Alex , Troyanskaya Olga G. 
Published: June 2016

AbstractAutism spectrum disorder (ASD) is a range of major neurodevelopmental disabilities with a strong genetic basis. Yet, owing to extensive genetic heterogeneity, multiple modes of inheritance and limited study sizes, sequencing and quantitative genetics approaches have had limited success in characterizing the complex genetics of ASD. Currently, only a small fraction of potentially causal genes—about 65 genes out of an estimated severalhundred—are known based on strong genetic evidence. Hence, there isa critical need for complementary approaches to further characterize the genetic basis of ASD, enabling development of better screening and therapeutics. Here, we use a machine-learning approach based on a human brain-specific functional gene interaction network to present a genome-wide prediction of autism-associated genes, including hundreds of candidate genes for which there is minimal or no prior genetic evidence. Our approach is validated in an independent case-control sequencing study of approximately 2,500families. Leveraging these genome-wide predictions and the brain-specificnetwork, we demonstrate that the large set of ASD genes converges on a smaller number of key cellular pathways and specific developmental stages of the brain. Specifically, integration with spatiotemporal transcriptome expression data implicates early fetal and midfetal stages of the developing human brain in ASD etiology. Likewise, analysis of the connectivity of topautism genes in the brain-specific interaction network reveals the breadthof autism-associated functional modules, processes, and pathways in the brain. Finally, we identify likely pathogenic genes within the most frequent autism-associated copy-number-variants (CNVs) and propose genes and pathways that are likely mediators of autism across multiple CNVs. All the predictions, interactions, and functional insights from this work are available to biomedical researchers atasd.princeton.edu.
The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance
Authors: Jiang Yanjuan , Yu Diqiu 
Published: June 2016

Abstract
Although necrotrophic pathogens cause many devastating plant diseases, our understanding of the plant defense response to them is limited. Here, we found that loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection. Further investigation suggested that the negative regulation of WRKY57 against B. cinerea depends on the jasmonic acid (JA) signaling pathway. Chromatin immunoprecipitation experiments revealed that WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription. In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2. Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY33 and WRKY57. Moreover, transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5, SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33. Therefore, coordinated regulation of Arabidopsis against B. cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense.
An Innate Immunity Pathway in the Moss Physcomitrella patens
Authors: Bressendorff Simon , Azevedo Raquel , Kenchappa Chandra Shekar , Ponce de León Inés , Olsen Jakob V. , Rasmussen Magnus Wohlfahrt , Erbs Gitte , Newman Mari-Anne , Petersen Morten , Mundy John 
Published: June 2016

Abstract
MAP kinase (MPK) cascades in Arabidopsis thaliana and other vascular plants are activated by developmental cues, abiotic stress, and pathogen infection. Much less is known of MPK functions in nonvascular land plants such as the moss Physcomitrella patens. Here, we provide evidence for a signaling pathway in P. patens required for immunity triggered by pathogen associated molecular patterns (PAMPs). This pathway induces rapid growth inhibition, a novel fluorescence burst, cell wall depositions, and accumulation of defense-related transcripts. Two P. patens MPKs (MPK4a and MPK4b) are phosphorylated and activated in response to PAMPs. This activation in response to the fungal PAMP chitin requires a chitin receptor and one or more MAP kinase kinase kinases and MAP kinase kinases. Knockout lines of MPK4a appear wild type but have increased susceptibility to the pathogenic fungi Botrytis cinerea and Alternaria brassisicola. Both PAMPs and osmotic stress activate some of the same MPKs in Arabidopsis. In contrast, abscisic acid treatment or osmotic stress of P. patens does not activate MPK4a or any other MPK, but activates at least one SnRK2 kinase. Signaling via MPK4a may therefore be specific to immunity, and the moss relies on other pathways to respond to osmotic stress.
Implications of cascading effects for the Hyogo Framework
Authors: McGee Sibel , Frittman Jaime , Ahn Seongjin James , Murray Susan 
Published: June 2016

Purpose
This paper aims to provide a preliminary systemic portrayal of risk relationships in the context of critical infrastructures (CIs) during disasters and assess the adequacy of the Hyogo Framework in addressing such relationships and the resultant cascading effects.

Design/methodology/approach
Using a systems thinking approach, this study views CIs as complex systems operating in the context of broader societies and disaster conditions. Using a causal loop diagramming technique, relationships across a large number of variables are mapped to capture pathways for cascading effects across CIs. This theoretical understanding is supplemented by cascading effects seen during the 2011 Japanese disaster. The Hyogo Framework indicators are mapped both to causal variables and disaster events to identify gaps. Data on cascading effects were collected from journals, news articles and reports by governments and NGOs.

Findings
The Hyogo Framework does not address facilitation by the host country of international aid during disasters; identification of infrastructure interdependencies and prioritization planning for recovery operations; national risk assessments that account for interrelated disasters; and private sector’s need to understand CIs’ dependencies and establish robust continuity plans that account for potential infrastructure failures.

Originality/value
This paper is the first attempt to assess the Hyogo Framework’s potential in addressing risk relationships and cascading effects. The knowledge provided in the paper is derived from the synthesis of previous cascading effects’ literature and examination of a real-life disaster. Findings are applicable to any disaster risk reduction initiative that seeks to anticipate and mitigate risk relationships and their implications for CIs during disasters.