bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMILIHAN KONTRASEPSI BERDASARKAN EFEK SAMPING PADA DUA KELOMPOK USIA REPRODUKSI
Authors: Setiawati Erna , Handayani Oktia Woro Kasmini , Kuswardinah Asih 
Published: September 2017

ABSTRACT
Kelompok usia reproduksi terbagi dalam tiga fase yaitufase menunda kehamilan (<20 tahun), fase menjarangkan kehamilan (20-30 tahun) dan fase mengakhiri kehamilan (>30 tahun). Cara yang ditempuh yaitu dengan pemakaian kontrasepsi.baik MKJPmaupunnon MKJP. Tujuan penelitian ini adalah untuk mengetahui ada atau tidak perbedaan pemilihan kontrasepsi MKJP dan non MKJP berdasarkan efek samping pada dua kelompok usia reproduksi. Penelitin ini menggunakan desain cross sectional, pengambilan data dengan kuesioner. Sampel dalam penelitian ini adalah akseptor KB baik MKJP maupun non MKJP pada bulan april sampai juni sebanyak 200 responden, dimana tekhnik pengambilan datanya dengan random sampling dan kuota sampling. Hasil penelitian kemudian diuji dengan mann-whitney test.Hasil penelitian dengan uji mann whitney test diperoleh p = 0.662 dengan kata lain p > α (0.05) yang berarti tidak ada perbedaan pemilihan MKJP dan non MKJP berdasarkan efek samping di Wilayah Kabupaten Semarang.

ABSTRACT
Reproductive-age category can be divided into three groups which are the group of delayed interval pregnancy (less than 20 years old), the group of intervalcontrol pregnancy (20 to 30 years old), and the group of high risk pregnancy (more than 30 years old). An alternative to avoid high risk pregnancy is by using contraception tool namely long-term contraception (MKJP) and non long-term contraception (non MKJP).The purpose of this research is to analysedwhether there are differences in choosing MKJP and non –MKJP based on side effects in the two reproductive-age groups.This research was an explanatory research with cross-sectional design. The population were all women of contraception acceptors in Semarang Regency.The samples were 200 respondents, used simple random sampling and quota sampling. This research used quisionaire instrument and analyze used mann whitney test (α=0,05). Theresult showed thatP = 0,662 meaning P > α = 0.05 which means there is no difference in choosing MKJP and non-MKJP based on side effects in the two reproduction-age groups in Semarang regency.
ID: 4001 Nanoparticle-based Cancer Therapy
Authors: Tamanoi Fuyu 
Published: September 2017

Advances in Nanotechnology have led to the development of a variety of nanomaterials that are changing the way cancer therapy is carried out. A particularly important example is nanoparticle that can carry cargo to tumor. We are using mesoporous silica nanoparticles (MSNs) for cancer therapy. MSNs contain thousands of pores that provide storage space for anticancer drugs. These materials are biocompatible and safe. In addition, we have recently introduced biodegradability into MSNs.
We have shown that MSNs exhibit excellent tumor targeting capability in two different animal model systems (chicken egg tumor model and mouse xenografts). This tumor targeting capability is partly due to its small size; these nano-sized particles can accumulate in tumor due to leaky tumor vasculature. In addition, we have carried out surface modifications to attach ligands that bind receptors present on the surface of cancer cells. For example, folate was attached to the surface that enables binding to folate receptors overexpressed on cancer cells.
We have also conferred controlled anticancer drug release capability to MSNs in collaboration with Fraser Stoddart and Jeff Zink. This was accomplished by attaching nanovalves at the opening of the pores. Rotaxanes and pseudorotaxanes are used to prepare nanovalves. These chemical compounds consist of a stalk and a moving part. When the moving part is close to the pore opening, the nanovalve is closed. On the other hand, when the moving part is located away from the pore opening, the nanovalve is closed. In this way, the nanovalve provides an open and close function so that controlled release of anticancer drugs can be carried out.
Light activated nanovalves were developed by incorporating azobenzene into nanovalves. Azobenzene changes conformation upon light exposure and this conformational change opens the nanovalve releasing anticancer drugs in a power and exposure time dependent manner. More recently, this system was modified by incorporating two-photon dyes that can capture energy from two-photon light and transfer to azobenzene to drive the release of anticancer drugs. This enables the system to work with tissue penetrating two-photon light.
We have also developed nanoparticles that respond to oscillating magnetic field. This system was developed using MSNs that contain iron oxide core. Because of superparamagnetic property of iron oxide, the internal temperature of such nanoparticles increases when exposed to oscillating magnetic field. This temperature increase drives opening of nanovalves that are particularly designed for this purpose.
Development of nanoparticles that respond to external cues such as light and magnetic field may change the way cancer therapy is carried out. Implications on the future of cancer therapy will be discussed.
ID3007 Mechanisms underlying the anticancer activities of the selected phytochemicals and their therapeutic implication in AGS gastric cancer cells
Authors: Cho Somi Kim 
Published: September 2017

The cysteine-rich angiogenic inducer 61 (CYR61), an extracellular matrix-associated protein, is involved in survival, tumorigenesis, and drug resistance. There is an increasing demand for developing agents that target CYR61. Hence, we study the effects of flavones against CYR61-overexpressing human gastric adenocarcinoma AGS (AGS-cyr61) cells. Among the various flavones, quercetin had lowest IC50 value and reduced the viability of AGS-cyr61 cells even greater than that of AGS cells. Quercetin (1) down-regulates CYR61 and concomitantly decreases in the levels of MRP1 (multidrug resistance-associated protein 1) and nuclear factor NF-kappa B (κB) p65 subunit, (2) reverses multidrug resistance, and (3) inhibits colony formation in AGS-cyr61 cells. AGS-cyr61 cells treated with quercetin at sub IC50 over a range of 5-FU or ADR concentrations manifested strong synergistic effects with these two drugs. Our results demonstrate that CYR61 is a potential regulator of ABC transporters and quercetin can be the novel agent that improves the efficacy of anticancer drugs by down-regulating CYR61 and ABC transporters.
Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme which contributes to malignant progression in various cancer. Such effect on cancer brings more interest on developing HDAC6 inhibitors. Here, we found that compound D inhibits HDAC6 activity, increases acetylated α-tubulin, reduces the level of β-catenin, and suppresses cell proliferation. Increase of α-tubulin acetylation by compound D resulted in tubulin polymerization, and consequently, induced aberrant mitosis. Moreover, DTBP elicits different effects depending on different concentrations.

Treatment with high concentrations of compound D induces cell death by mitotic catastrophe, whereas low concentration of compound D induces senescence with upregulation of p21 and Rb, and increase in the phosphorylation of mTOR and the β-galactosidase activity. Therefore, compound D can also be considered as a promising new candidate for anti-cancer drug development.
ID3008 Differential expression of circadian genes in leukemia and a possible role for Sirt1 in restoring the circadian clock in chronic myeloid leukemia
Authors: Boudjelal Mohamed 
Published: September 2017

Dis-regulation of genes making up the mammalian circadian clock has been associated with different forms of cancer. This study initially aimed to address how the circadian clock genes behave over the course of treatment for both the acute and chronic forms of leukemia and whether any could be used as potential biomarkers as a read-out for therapeutic efficacy. Expression profiling for both core and peripheral clock genes revealed that the majority of clock genes are down-regulated in AML patients apart from Cry2 which is up-regulated towards the end of treatment. A similar observation was seen in ALL patients however here, Cry2 expression came back up towards control levels upon treatment completion. In addition, all of the core clock genes are down-regulated in both chronic forms of leukemia (CML and CLL) apart from Cry2 which is not affected when the disease is diagnosed. Furthermore, the NAD(+) – dependent protein deacetylase Sirt1 has been proposed to have a dual role in both control of circadian clock circuitry as well as promotion of cell survival by inhibiting apoptotic pathways in cancer. We used a pharmacological-based approach to see whether Sirt1 played a role in regulating the circadian clock circuitry in both Acute and Chronic forms of leukemia. Our results suggest that interfering with Sirt1 leads to a partial restoration of BMAL1 oscillation in CML patient samples. Furthermore, interfering with Sirt1 activity leads to both the induction and repression of circadian clock genes in both Acute and chronic forms of leukemia and therefore makes it a potential therapeutic target to either augment existing therapies for chronic leukemia or to act as a means of facilitating chronotherapy in order to maximize both the effectiveness of existing therapies and to minimize therapy-associated toxicity.
ID3013 Comparative characterization of murine Bone marrow mesenchymal stem cells cultured using two different supplements
Authors: Thilakavathy Karuppiah 
Published: September 2017

Preclinical studies on mesenchymal stem cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. The mouse is the most widely used species for studying the characteristics of MSC. In recent years, conflicting data were reported regarding growth kinetics, surface marker profile, differentiation capacity, genetic instability or malignant transformation and so forth, that may be a result of a range of factors. One of the factors probably is the culture medium formulation. Here we have made a comparative characterization of bone marrow-derived mesenchymal stem cells (mBM-MSC), under the same experimental conditions, cultured using two common supplements, fetal bovine serum (FBS) and MesenCultTM Stimulatory Supplement (MSS). mBM-MSC isolated from the tibias of C57BL/6 mice were cultured and expanded in Dulbecco’s Modified Eagle’s Medium supplemented with either 15% FBS or 15% MSS. Clonogenic potential, population doubling time, immunophenotyping, differentiation immunosuppression potentials and chromosome analysis of early and late passage of mBM-MSC were assessed.
The findings showed that the immunophenotype and differentiation potential of mBM-MSC were similar when cultured using these supplements irrespective of passages. Variations were seen in clonogenic, growth, proliferation rate and immunosuppression potential of the mBM-MSC. This study also revealed that prolonged culture will disrupt their genetic stability regardless of the supplements used. The genetically mutated mBM-MSC were also found to maintain their stemness characteristics and immunosuppression potential.
In conclusion, culture medium formulation causes variations in the cultured MSC and may influence downstream investigation findings.
ID: 1012 Cytotoxicity of Corchorus olitorius Linn. aqueous leaf extract against human carcinoma cells
Authors: Tosoc John Paul 
Published: September 2017

C. olitorius are used as herbal medicine and eaten as vegetable by local people in Philippines. The T’boli tribe commonly uses this plant to treat common illnesses such as pimples, wounds, boils, and inflammations. According to studies, the leaves of C. olitorius has properties to treat demulcent, diuretic, febrifuge, chronic cystitis, dysuria and even tumor. The aim of this study is to evaluate the cytotoxicity properties of the crude aqueous extract of C. olitorius leaf against colon (HCT116), breast (MCF-7), and liver (HepG2) carcinoma cell-lines using the MTT cell proliferation assay. The results showed that the aqueous extract has potential cytotoxicity activity against the three-carcinoma cell-lines. The aqueous extract was most bioactive in the colon carcinoma cell-line (HCT116) with cell viability percentage of 23.53 and 32.48 in 100 and 10 µg/mL, respectively. It was then followed by the breast carcinoma cell-line (MCF7) with cell viability percentage of 16.23 and 30.25 in 100 and 10 µg/mL, respectively. The extract was least but still bioactive in the liver carcinoma cell-line (HepG2) with cell viability percentage of 91.20 and 132.76 in 100 and 10 µg/mL, respectively. This study illustrates the importance of toxicological screening to confirm the safety and efficacy of the medicinal plant commonly used in the traditional medicine system in the treatment and management of illnesses by the T’boli tribe in South Cotabato
ID: 1034 Potential cancer predictive biomarkers: a case of papillary thyroid cancer
Authors: Gerić Marko , Janušić Renato , Šarčević Božena , Garaj-Vrhovac Vera 
Published: September 2017

Thyroid cancer represents 2.1% of new cancer cases, yet it is the most common endocrine cancer and one of the fastest growing cancer types in the world. The incidence of thyroid cancer is the highest in elderly population (60-69 years), but novel data suggest decline in age when this cancer is frequently diagnosed to 50-59 years. Thyroid cancer is 2-4-fold more common in female population what is usually associated with female hormones and the differences in oxidative stress levels. Since the WHO suggest that roughly 30% of cancer can be prevented, the aim of this study was to evaluate the role of micronucleus test and comet assay as cancer predictive biomarkers.
In this study, 36 patients with papillary thyroid cancer (PTC) were recruited in accordance with all ethical standards. The average age of the group was 52±14 years, 28:8 female:male ratio, and 33% of active smokers. Control group was matched for age, gender, and smoking habits (52±14 years, 28:8 female:male ratio, and 33% of active smokers).
The number of micronucleated (MNed) cells in human peripheral blood lymphocytes was significantly higher (p<0.05) in PTC group (12.94±5.81) compared to control group (4.36±2.58). Additionally, the comet assay tail moment (TM) was also significantly (p<0.05) higher in the PTC group compared to control group (0.39±0.71 vs 0.14±0.05). These results indicate that people with PTC had more genome damage compared to healthy controls. The immunohistochemical analysis of thyroid revealed 97.2% BRAF positive and 20.6% RET/PTC positive samples.
Taken together, the PTC group had more DNA damage what is associated with high prevalence of BRAF mutations and RET/PTC translocations as a consequence of disrupted genome integrity. The results imply that cytogenetic tools might be used in human biomonitoring for identification of high-risk groups but further research is needed to assess normal and cut-off values.
ID: 1035 Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection
Authors: Le Trinh Van , Nguyen Nam Hai , Do Huy Quang , Truong Nhung Hai 
Published: September 2017

Background: Up to date, there have been some studies indicating positive effects of stem cells on treating the liver cirrhosis. In this study, we compared the effectiveness of two methods in which mesenchymal stem cells harvested from umbilical cord blood (UCB-MSCs) were transfused either via portal or tail veins to the mouse models of liver cirrhosis.
Methods: Liver cirrhosis was induced by CCl4 (1 ml/kg) on male Swiss mice within 11 weeks, followed by administration of 106 UCB-MSCs via the portal or tail vein. After 21 days, blood samples were collected for measuring transaminase, bilirubin and albumin activities. The expression of fibrosis-associated genes, specifically procollagen – alpha 1 and integrin – beta1, were assessed using qRT-PCR. The histopathology was also evaluated using hematoxylin/eosin, Masson trichrome staining and immunohistochemistry with collagen type 1 and alpha-SMA antibody.
Results: UCB-MSCs transplantation significantly improved post-21 days of treatment in the liver fibrosis mice as compared with placebo group. Notably, UCB-MSCs transferred through portal veins revealed a more positive effect than via tail veins as indicated by the improvement in the biochemical indexes, fibrosis-related genes expression, and liver histopathology.
Conclusion: The UCB-MSCs therapy proved to be a promising method for treating the liver cirrhosis. The method of delivering stem cells through portal vein was more effective than through tail vein
ID: 1042 Production of PHA from Pseudomonas Plecoglossicida and its biomedical application
Authors: Devaraj Sabarinathan 
Published: September 2017

To reduce the amount of plastic waste, world-wide programs for efficient management of utilized plastic materials, such as recycling, have been started PHAs could be completely relied on replacing the conventional plastics as they share similar thermo mechanical properties with the petrochemical based plastics. PHA exhibit high level of biocompatibility and biodegradability, hence they are used for several biomedical applications. PHA extracted from Pseudomonas plecoglossicida checked for its ability to attach cancer cells was investigated. Cancer cells showed high affinity of adherence on the PHA sheets used. Contact angle detection have proved that only cancer cells showed the adherence effect and normal cells did not exhibit such adherence property towards the PHA sheets which was further authenticated by contact angle observations. Hence, PHA sheets can be effectively used as a valid target for the contact angle detection of cancer cells.
ID: 1070 Single-Cell Gene Expression Profiling in Breast Cancer Cells with the HER2/neu Gene Knockout by CRISPR-Cas9
Authors: Wang Xiaoyang (Alice) , Lomas Chip , Tycon Michael A. , Lam Regina , Weaver Suzanne 
Published: September 2017

The HER2/neu gene is amplified and overexpressed in 15–30% of breast cancers. The overexpression of this oncogene is strongly correlated with decreased survival, increased cancer relapse, and poor prognosis. Therapy has been successfully developed to target this oncogene, yet a better understanding of this oncogene will provide further insight for breast cancer biology and future drug development. In recent years, CRISPR-Cas9 has emerged as an efficient method for genetic engineering, allowing for targeted gene knockout with minimal off-target effects.
In this study, we employed the CRISPR-Cas9 system to knock out the HER2/neu gene in breast cancer cells. Plasmids with guide RNAs, and Cas9, were transfected into the cells to knock out the HER2 gene. The transfected cells were selected by drug resistance. Then, the cells were sorted into the wells of a 96-well BD™ Precise plate. A Whole transcriptome amplification (WTA) assay was performed to obtain a gene expression profile for each cell. In this study, the HER2 gene knockout was performed on T47D, a ductal carcinoma derived breast cancer cell line with no HER2 amplification that shows low to intermediate HER2/neu expression, as well as SKBR3, an adenocarcinoma derived breast cancer cell line known for HER2/neu gene amplification and overexpression. Parental cells without gene editing were also sorted as individual cells for gene expression profiling comparison. The profiling of gene expression in HER2/neu CRISPR-Cas9 knockout cells on a single-cell level might give us insight into the mechanisms in these aggressive cancers that could help future drug discovery for these aggressive cancer types.
ID: 1077 Overexpress of CD47 does not alter stemness of MCF-7 breast cancer cells
Authors: Nguyen Oanh Thi-Kieu , Bui Anh Nguyen Tu , Vu Ngoc Bich , Pham Phuc Van 
Published: September 2017

Background: CD47 is a transmembrane glycoprotein expressed on all cells in the body and particularly overexpressed on cancer cells and cancer stem cells of both hematologic and solid malignancies. In the immune system, CD47 acts as a "don't eat me" signal, inhibiting phagocytosis by macrophages by interaction with signal regulatory protein α (SIRPα). In cancer, CD47 promotes tumor invasion and metastasis. This study aimed to evaluate the stemness of breast cancer cells when CD47 is overexpressed.
Methods: MCF-7 breast cancer cells were transfected with plasmid pcDNA3.4-CD47 containing the CD47 gene. The stemness of the transduced MCF7 cell population was evaluated by expression of CD44 and CD24 markers, anti-tumor drug resistance and mammosphere formation
Results: Transfection of plasmid pcDNA3.4-CD47 significantly increased the expression of CD47 in MCF-7 cells. The overexpression of CD47 in transfected MCF-7 cells led to a significant increase in the CD44+CD24- population, but did not increase doxorubicin resistance of the cells or their capacity to form mammospheres.
Conclusion: CD47 overexpression enhances the CD44+CD24- phenotype of breast cancer cells as observed by an increase in the CD44+CD24- expressing population. However, these changes are insufficient to increase the stemness of breast cancer cells.
ID: 1078 Autologous and allogeneic transplantation of adipose derived stem cells have similar effects for type 1 diabetes mellitus treatment in mouse models
Authors: Bui Anh Nguyen Tu , Nguyen Oanh Thi-Kieu , Nguyen Cong Le Thanh , Dang Loan Thi-Tung , Pham Phuc Van , Phan Ngoc Kim 
Published: September 2017

Background: Type 1 diabetes mellitus (T1D) disease is caused by lesions or dysfunction of beta cells of pancreatic islets, causing less insulin to be secreted into the blood and thereby increasing glucose levels in the blood. In this study, we evaluated and compared the efficiency of treatment for T1D using autograft and allograft adipose-derived stem cells (ADSCs).
Methods: ADSCs were collected from the belly of mice before they were injected using a single dose of streptozotocin (100 mg/kg) to induce T1D. T1D mice were intravenously injected with a dose of 2x106 ADSCs into the tail vein. Therapeutic efficacy was assessed by survival rate, blood glucose levels, serum insulin levels, histology and immunohistochemistry of pancreatic islets.
Results: The results showed that both autograft and allograft transplantation of ADSCs demonstrated similarities in mortality rate, blood glucose level, blood insulin level, quantity and size of pancreatic islets. Both transplantations significantly improved T1D mice, which showed a decrease in mortality rate as well as blood glucose level, and increases in blood insulin level, quantity and size of pancreatic islets.
Conclusion: The similar results suggest that both autologous and allogeneic transplantations of ADSCs are promising therapy for T1D treatment.