bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phage-mediated dissemination of virulence factors in pathogenic bacteria facilitated by antibiotic growth promoters in animals: a perspective
Authors: Tamang Migma Dorji , Sunwoo Hoon , Jeon Byeonghwa 
Published: November 2017

AbstractAddition of sub-therapeutic antibiotics to the feed of food-producing animals for growth promotion and disease prevention has become a common agricultural practice in many countries. The emergence of antibiotic-resistant pathogens is a looming concern associated with the use of antibiotic growth promoters (AGPs) around the world. In addition, some studies have shown that AGPs may not only affect antibiotic resistance but may also stimulate the dissemination of virulence factors via bacteriophages. Although only a few studies are currently available in the literature regarding this topic, in this article we endeavor to provide a perspective about how AGPs would impact the transmission of virulence factors by horizontal gene transfer via phages in a few pathogenic bacterial species significant to livestock production.
Productivity and profitability of manual and mechanized conservation agriculture (CA) systems in Eastern Zambia
Authors: Mupangwa W. , Mutenje M. , Thierfelder C. , Mwila M. , Malumo H. , Mujeyi A. , Setimela P. 
Published: November 2017

AbstractClimate variability and declining soil fertility pose a major threat to sustainable agronomic and economic growth in Zambia. The objective of this study was to assess crop yield, land and labor productivity of conservation agriculture (CA) technologies in Eastern Zambia. On-farm trials were run from 2012–2015 and farmers were replicates of a randomized complete block design. The trials compared three CA systems against a conventional practice. Yield and net return ha−1were determined for maize and legume yield (kg ha−1) produced by ridge and furrow tillage, CA dibble stick planting, CA animal traction ripping and direct seeding. The dibble stick, ripline and direct seeding CA systems had 6–18, 12–28 and 8–9% greater maize yield relative to the conventional tillage system, respectively. Rotation of maize with cowpea and soybean significantly increased maize yields in all CA systems. Intercropping maize with cowpea increased land productivity (e.g., the land equivalent ratio for four seasons was 2.01) compared with full rotations under CA. Maize/cowpea intercropping in dibble stick CA produced the greatest net returns (US$312-767 ha−1) compared with dibble stick maize-cowpea rotation (US$204-657), dibble stick maize monoculture (US$108-584) and the conventional practice (US$64-516). The net-return for the animal traction CA systems showed that maize-soybean rotations using the ripper were more profitable than the direct seeder or conventional ridge and furrow systems. Agronomic and economic benefits of CA-based cropping systems highlight the good potential for improved food security and agricultural productivity for smallholder farmers.
Subchronic olanzapine exposure leads to increased expression of myelination-related genes in rat fronto-medial cortex
Authors: Ersland Kari M. , Skrede Silje , Stansberg Christine , Steen Vidar M. 
Published: November 2017

AbstractSchizophrenia is a psychotic disorder with severe and disabling symptoms, such as hallucinations, delusions, blunted affect and social withdrawal. The neuropathology remains elusive, but disturbances in immunity-related processes, neuronal connectivity and myelination have consistently been linked to schizophrenia. Antipsychotic drugs can be efficient in reducing symptoms, acting primarily on the dopamine system, but additional biological targets are likely to exist. Here we have screened for novel mechanisms of action in an animal model, using adult rats exposed to long-acting olanzapine, achieving stable and clinically relevant antipsychotic drug concentrations. By microarray-based examination of global gene expression in the fronto-medial cortex, at the single gene- and gene-set level, we observed downregulation of two neuropeptide-encoding genes, Vgf and Cort (fold change −1,25 and −1,48, respectively) in response to olanzapine exposure. Furthermore, we demonstrated significant upregulation of five out of ~2000 GO predefined gene sets after olanzapine exposure. Strikingly, all were linked to myelination and oligodendrocyte development; “Ensheathment of neurons”, “Axon ensheathment”, “Myelination”, “Myelin sheath” and “Oligodendrocyte development” (FDR-values < 25). Sixteen of the leading edge genes in these gene sets were analysed independently by qPCR, of which 11 genes displayed significant upregulation, includingPlp1,Mal,MagandCnp(fold change: 1,30, 1,50, 1,30 and 1,15, respectively). Several of the upregulated genes (e.g.MAG,MALandCNP) have previously been reported as downregulated in post-mortem brain samples from schizophrenia patients. Although caution needs to be taken when extrapolating results from animal studies to humans, the data suggest a role for olanzapine in alleviating myelination-related dysfunction in schizophrenia.
Genome-wide haplotype-based association analysis of major depressive disorder in Generation Scotland and UK Biobank
Authors: Howard David M. , Hall Lynsey S. , Hafferty Jonathan D. , Zeng Yanni , Adams Mark J. , Clarke Toni-Kim , Porteous David J. , Nagy Reka , Hayward Caroline , Smith Blair H. , Murray Alison D. , Ryan Niamh M. , Evans Kathryn L. , Haley Chris S. , Deary Ian J. , Thomson Pippa A. , McIntosh Andrew M. 
Published: November 2017

AbstractGenome-wide association studies using genotype data have had limited success in the identification of variants associated with major depressive disorder (MDD). Haplotype data provide an alternative method for detecting associations between variants in weak linkage disequilibrium with genotyped variants and a given trait of interest. A genome-wide haplotype association study for MDD was undertaken utilising a family-based population cohort, Generation Scotland: Scottish Family Health Study (n = 18,773), as a discovery cohort with UK Biobank used as a population-based replication cohort (n = 25,035). Fine mapping of haplotype boundaries was used to account for overlapping haplotypes potentially tagging the same causal variant. Within the discovery cohort, two haplotypes exceeded genome-wide significance (P < 5 × 10−8) for an association with MDD. One of these haplotypes was nominally significant in the replication cohort (P < 0.05) and was located in 6q21, a region which has been previously associated with bipolar disorder, a psychiatric disorder that is phenotypically and genetically correlated with MDD. Several haplotypes with P < 10−7 in the discovery cohort were located within gene coding regions associated with diseases that are comorbid with MDD. Using such haplotypes to highlight regions for sequencing may lead to the identification of the underlying causal variants.
Diffusion of treatment in social networks and mass drug administration
Authors: Chami Goylette F. , Kontoleon Andreas A. , Bulte Erwin , Fenwick Alan , Kabatereine Narcis B. , Tukahebwa Edridah M. , Dunne David W. 
Published: November 2017

AbstractInformation, behaviors, and technologies spread when people interact. Understanding these interactions is critical for achieving the greatest diffusion of public interventions. Yet, little is known about the performance of starting points (seed nodes) for diffusion. We track routine mass drug administration—the large-scale distribution of deworming drugs—in Uganda. We observe friendship networks, socioeconomic factors, and treatment delivery outcomes for 16,357 individuals in 3491 households of 17 rural villages. Each village has two community medicine distributors (CMDs), who are the seed nodes and responsible for administering treatments. Here, we show that CMDs with tightly knit (clustered) friendship connections achieve the greatest reach and speed of treatment coverage. Importantly, we demonstrate that clustering predicts diffusion through social networks when spreading relies on contact with seed nodes while centrality is unrelated to diffusion. Clustering should be considered when selecting seed nodes for large-scale treatment campaigns.
Bioaccumulation of pharmaceuticals and personal care product chemicals in fish exposed to wastewater effluent in an urban wetland
Authors: Muir Derek , Simmons Denina , Wang Xiaowa , Peart Tom , Villella Maria , Miller Jason , Sherry Jim 
Published: November 2017

AbstractThe bioaccumulation of a broad range of pharmaceuticals and personal care product chemicals (PPCPs) was studied in Cootes Paradise Marsh (CPM), an urban wetland that receives tertiary treated municipal waste waters as well as urban storm runoff. We measured PPCPs in caged and wild goldfish, as well as wild carp, and compared observed bioaccumulation factors (BAFP) using concentrations in surface waters and fish blood plasma, with modeled BAFs. Thirty-two PPCPs were detected in water from the central CPM site (CPM3) while 64 PPCPs were found at higher concentrations at a site immediately downstream of the effluent outflow (CPM1). Following a 3-week deployment, 15 PPCPs were detected in the plasma of caged goldfish at CPM1, and 14 at CPM3, compared to only 3 in goldfish caged at a reference site. The highest BAFP in goldfish were for the antidepressant Σfluoxetine averaging 386 L/kg in caged and 906 L/kg in wild goldfish, respectively. In carp, ΣDiazepam (diazepam and oxazepam) had the highest BAFP (927 L/kg). This study identified a broader range of PPCPs in fish and surface waters than previously reported. However, modeled BAFs did not show good agreement with observed whole body or plasma BAFs, demonstrating that more work is needed to better explain bioaccumulation of PPCPs.
Design and Synthesis of an Artificial Perpendicular Hard Ferrimagnet with High Thermal and Magnetic Field Stabilities
Authors: Lu Jun , Mao Siwei , Zhao Xupeng , Wang Xiaolei , Liu Jian , Xia Jianbai , Xiong Peng , Zhao Jianhua 
Published: November 2017

AbstractIt is of great fundamental and practical interest to develop effective means of modulating the magnetic hystereses of magnetic materials and their heterostructures. A notable example is the exchange bias (EB) effect between an antiferromagnet or ferrimagnet and a ferromagnet, which has been widely employed to manipulate magnetic anisotropy in spintronic devices and artificial magnets. Here, we report the design, synthesis and characterization of a synthetic perpendicularly-magnetized ferrimagnet based on [Mn2.9Ga/Co2MnSi]n superlattices, which attains thermal stability above 400 K and a coercive field up to 45 kOe through a mechanism of magnetic compensation. The structure is incorporated into a prototype Heusler alloy and MgO barrier based magnetic tunnel junction, which demonstrates high dynamic range linear field responses and an unusual in-plane EB effect. With increasing temperature, the coercive field reaches beyond 70 kOe at 400 K in this device due to the increasing degree of magnetic moment compensation in the superlattice. The results demonstrate that the compensation mechanism can be utilized to achieve simultaneous thermal robustness and high coercivity in realistic spintronic devices.
Reconstructing ice-margin retreat using delta morphostratigraphy
Authors: Dietrich Pierre , Ghienne Jean-François , Normandeau Alexandre , Lajeunesse Patrick 
Published: November 2017

AbstractThe paleogeographic reconstruction of the successive inland positions of a retreating ice sheet is generally constrained by mapping moraines. However, deltaic complexes constructed by sediment-charged meltwater can also provide a record of the retreating ice-margin positions. Here, we examine a serie of ice-contact, ice-distal glaciofluvial and paraglacial depositional systems that developed along the Québec North Shore (eastern Canada) in the context of falling relative sea level during the northward retreat of the Laurentide Ice Sheet (LIS). Ice-contact depositional systems formed when the LIS was stillstanding along the Québec North Shore. Subsequent inland retreat of the ice margin generated glacial meltwaters feeding sediment to glaciofluvial deltas, leading to their rapid progradation. The retreat of the ice margin from drainage basins was marked by the onset of paraglacial processes such as the shutdown of delta progradation, severe fluvial entrenchment, and deposition of shallow-marine strata. Four end-member scenarios describe the spatial and stratigraphic distribution of these three depositional systems (ice-contact deposits, ice-distal glaciofluvial deltas, and paraglacial suites). They reflect both the inherited drainage basin physiography and the retreat pattern of the ice margin. Applied to twenty deltaic complexes, these end-members allowed us to refine the model of LIS-margin retreat over southeastern Québec.
Alternative substrate-bound conformation of bacterial solute-binding protein involved in the import of mammalian host glycosaminoglycans
Authors: Oiki Sayoko , Kamochi Reiko , Mikami Bunzo , Murata Kousaku , Hashimoto Wataru 
Published: November 2017

AbstractGlycosaminoglycans (GAGs), constituted by repeating uronate and amino sugar units, are major components of mammalian extracellular matrices. Some indigenous and pathogenic bacteria target GAGs for colonization to and/or infection of host mammalian cells. In Gram-negative pathogenic Streptobacillus moniliformis, the solute-binding protein (Smon0123)-dependent ATP-binding cassette (ABC) transporter incorporates unsaturated GAG disaccharides into the cytoplasm after depolymerization by polysaccharide lyase. Smon0123, composed of N and C domains, adopts either a substrate-free open or a substrate-bound closed form by approaching two domains at 47° in comparison with the open form. Here we show an alternative 39°-closed conformation of Smon0123 bound to unsaturated chondroitin disaccharide sulfated at the C-4 and C-6 positions of N-acetyl-d-galactosamine residue (CΔ4S6S). In CΔ4S6S-bound Smon0123, Arg204 and Lys210 around the two sulfate groups were located at different positions from those at other substrate-bound 47°-closed conformations. Therefore, the two sulfate groups in CΔ4S6S shifted substrate-binding residue arrangements, causing dynamic conformational change. Smon0123 showed less affinity with CΔ4S6S than with non-sulfated and monosulfated substrates. ATPase activity of the Smon0123-dependent ABC transporter in the presence of CΔ4S6S was lower than that in the presence of other unsaturated chondroitin disaccharides, suggesting that CΔ4S6S-bound Smon0123 was unpreferable for docking with the ABC transporter.
Chitosan coated cotton cloth supported zero-valent nanoparticles: Simple but economically viable, efficient and easily retrievable catalysts
Authors: Ali Fayaz , Khan Sher Bahadar , Kamal Tahseen , Alamry Khalid A. , Asiri Abdullah M. , Sobahi Tariq R. A. 
Published: November 2017

AbstractA simple, economically viable and fast method has been utilized for the preparation of highly active metal nanoparticles (MNPs) in coating layer of chitosan (CH) over cellulose microfibers of cotton cloth (CC). 2 wt% of CH solution was used for the coating of CC strips (CC-CH), and were kept in aqueous solutions of metal salts to adsorb metal ions. The CC-CH templated with metal ions were then treated with aqueous solution of NaBH4 to reduce the metal ions into zero-valent metal nanoparticles (M0). The CC-CH strips loaded with M0 were characterized by XRD, XPS, ATR-FTIR, FE-SEM and TGA, which indicates the successful synthesis of MNPs by this method. The M0/CC-CH strips were used as an efficient catalyst for the model reduction reaction of nitrophenol and toxic organic dyes. Among all the prepaped samples, Fe/CC-CH showed good catalytic activity for 4-NP and Rh-B dye reduction in the presence of NaBH4 with rate constants of 0.2937 min−1 and 0.3804 min−1, respectively. Moreover Fe/CC-CH has good catalytic reduction ability for MO and MB having rate constants equal to 0.1698 and 0.2802 min−1, respectively. Beside the good catalytic ability, it could be easily recoverable as compared to other available techniques. The recovery was completed by simply pulling the strip from the reaction matrix after completion of the reaction and can be used several times.
Influence of Spatial and Chromatic Noise on Luminance Discrimination
Authors: Miquilini Leticia , Walker Natalie A. , Odigie Erika A. , Guimarães Diego Leite , Salomão Railson Cruz , Lacerda Eliza Maria Costa Brito , Cortes Maria Izabel Tentes , de Lima Silveira Luiz Carlos , Fitzgerald Malinda E. C. , Ventura Dora Fix , Souza Givago Silva 
Published: November 2017

AbstractPseudoisochromatic figures are designed to base discrimination of a chromatic target from a background solely on the chromatic differences. This is accomplished by the introduction of luminance and spatial noise thereby eliminating these two dimensions as cues. The inverse rationale could also be applied to luminance discrimination, if spatial and chromatic noise are used to mask those cues. In this current study estimate of luminance contrast thresholds were conducted using a novel stimulus, based on the use of chromatic and spatial noise to mask the use of these cues in a luminance discrimination task. This was accomplished by presenting stimuli composed of a mosaic of circles colored randomly. A Landolt-C target differed from the background only by the luminance. The luminance contrast thresholds were estimated for different chromatic noise saturation conditions and compared to luminance contrast thresholds estimated using the same target in a non-mosaic stimulus. Moreover, the influence of the chromatic content in the noise on the luminance contrast threshold was also investigated. Luminance contrast threshold was dependent on the chromaticity noise strength. It was 10-fold higher than thresholds estimated from non-mosaic stimulus, but they were independent of colour space location in which the noise was modulated. The present study introduces a new method to investigate luminance vision intended for both basic science and clinical applications.
Type I interferons drive inflammasome-independent emergency monocytopoiesis during endotoxemia
Authors: Lasseaux Corentin , Fourmaux Marie-Pierre , Chamaillard Mathias , Poulin Lionel Franz 
Published: November 2017

AbstractEmergency monocytopoiesis is an inflammation-driven hematological process that supplies the periphery with monocytes and subsequently with macrophages and monocyte-derived dendritic cells. Yet, the regulatory mechanisms by which early bone marrow myeloid progenitors commit to monocyte-derived phagocytes during endotoxemia remains elusive. Herein, we show that type I interferons signaling promotes the differentiation of monocyte-derived phagocytes at the level of their progenitors during a mouse model of endotoxemia. In this model, we characterized early changes in the numbers of conventional dendritic cells, monocyte-derived antigen-presenting cells and their respective precursors. While loss of caspase-1/11 failed to impair a shift toward monocytopoiesis, we observed sustained type-I-IFN-dependent monocyte progenitors differentiation in the bone marrow correlated to an accumulation of Mo-APCs in the spleen. Importantly, IFN-alpha and -beta were found to efficiently generate the development of monocyte-derived antigen-presenting cells while having no impact on the precursor activity of conventional dendritic cells. Consistently, the LPS-driven decrease of conventional dendritic cells and their direct precursor occurred independently of type-I-IFN signaling in vivo. Our characterization of early changes in mononuclear phagocytes and their dependency on type I IFN signaling during sepsis opens the way to the development of treatments for limiting the immunosuppressive state associated with sepsis.
Single electron-photon pair creation from a single polarization-entangled photon pair
Authors: Kuroyama Kazuyuki , Larsson Marcus , Matsuo Sadashige , Fujita Takafumi , Valentin Sascha R. , Ludwig Arne , Wieck Andreas D. , Oiwa Akira , Tarucha Seigo 
Published: November 2017

AbstractQuantum entanglement between different forms of qubits is an indication of the universality of quantum mechanics. Entanglement transfer between light and matter, especially photon and spin, has long been studied as the central concept, but it remains technically challenging for single photons and spins. In this paper, we show paired generation of a single electron in a GaAs quantum dot and a single photon from a single polarization-entangled photon pair. We measure temporal coincidence between the single photo-electron detection and the single photon detection. Considering a single photon polarization is converted to an electron spin via an optical selection rule, the present result indicates the capability of photon to spin entanglement transfer. This may be useful to explore the physics of entanglement transfer and also for applications to quantum teleportation based quantum communication.
Impact of remotely generated eddies on plume dispersion at abyssal mining sites in the Pacific
Authors: Aleynik Dmitry , Inall Mark E. , Dale Andrew , Vink Annemiek 
Published: November 2017

AbstractProposed harvesting of polymetallic nodules in the Central Tropical Pacific will generate plumes of suspended sediment which are anticipated to be ecologically harmful. While the deep sea is low in energy, it also can be highly turbulent, since the vertical density gradient which suppresses turbulence is weak. The ability to predict the impact of deep plumes is limited by scarcity of in-situ observations. Our observations show that the low-energy environment more than four kilometres below the surface ultimately becomes an order of magnitude more energetic for periods of weeks in response to the passage of mesoscale eddies. The source of these eddies is remote in time and space, here identified as the Central American Gap Winds. Abyssal current variability is controlled by comparable contributions from tides, surface winds and passing eddies. During eddy-induced elevated flow periods mining-related plumes, potentially supplemented by natural sediment resuspension, are expected to spread and disperse more widely and rapidly. Predictions are given of the timing, location and scales of impact.
A simple method to assess group difference in RT-qPCR reference gene selection using GeNorm: The case of the placental sex
Authors: St-Pierre Joey , Grégoire Jean-Charles , Vaillancourt Cathy 
Published: November 2017

AbstractNormalization with proper reference genes is a crucial step in obtaining accurate mRNA expression levels in RT-qPCR experiments. GeNorm and NormFinder are two commonly used software packages that help in selecting the best reference genes, based on their expression stability. However, GeNorm does not take into account a group variable, such as sample sex, in its calculation. We demonstrate a simple calculation step to assess the variability of such parameters by multiplying the GeNorm M value with the difference of Cq values between groups. To test this, we used 28 reference gene candidates, to analyze 20 placental samples (10 of each sex), and by using HPRT1 (lower Cq values in male placentas (P = 0.017)), as a target gene. Our calculation demonstrates that the RPL30 – GAPDH reference gene combination is the better option to assess small placental sex differences in mRNA level, versus the selection obtained from GeNorm or NormFinder. The HPRT1 normalized mRNA expression level is different between placental sexes, using RPL30 and GAPDH as reference genes (P = 0.01), but not when using genes suggested by GeNorm or NormFinder. These results indicate that the proposed calculation is appropriate to assess small variations in mRNA expression between 2 groups.