bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Brucella melitensis wbkC protein affecting the lncRNA expression profiles of RAW264.7 cells and mining of autophagy pathway lncRNAs
Authors: Jiao Hanwei , Li Wenjie , Jiao Fengyuan , Zhou Gengxu , Meng Chi , Wang Lingjie , Wu Shengping , Fan Cailiang , Zhou Zuoyong , Cao Liting , Li Jixiang , Chu Yuefeng 
Published: September 2024

Abstract

Background
Brucellosis is a veterinary and human disease caused by Brucella, which not only causes serious public safety but also affects the development of animal husbandry and international trade. The formyl transferase encoded by the wbkC gene plays an important role in the synthesis of lipopolysaccharide (LPS), an important virulence factor of Brucella melitensis. Long-stranded non-coding RNAs (lncRNAs), as an emerging regulatory molecule, are thought to be extensively involved in the regulation of cellular activities. In this paper, by studying the analysis of Brucella melitensis. wbkC protein affecting the lncRNA expression profile of RAW264.7 cells and the mining of autophagy pathway lncRNAs in the sheep species, the aim of this study was to reveal the mechanism by which B. melitensis affects macrophage autophagy and intracellular survival.
Methods
In this study, B. melitensis wbkC protein was obtained by prokaryotic expression, and polyclonal antibody to wbkC protein was prepared by immunizing rabbits. Recombinant adenovirus for wbkC gene overexpression was prepared to mediate wbkC overexpression in RAW264.7 cells with Ad_wbkC. The wbkC protein was analyzed to affect the lncRNA expression profile of RAW264.7 cells by transcriptomics sequencing technology to mine the autophagy pathway-related lncRNAs.
Results
The results showed that Ad_wbkC successfully mediated the overexpression of wbkC in RAW264.7 cells, and a total of 32 expression up-regulated lncRNAs versus 30 expression down-regulated lncRNAs were identified in the Ad_wbkC group as compared with the control group. By analyzing the functional enrichment of the lncRNA target genes GO and KEGG, the lncRNAs with TOP15 expression related to autophagy were screened for qRT-PCR validation. The validation results showed that lncRNA 4933430A20Rik and lncRNA B930036N10Rik were consistent with the sequencing results.
Conclusions
B. melitensis wbkc protein further affects macrophage autophagy and intracellular survival by influencing lncRNA expression.
Prognosis of Pathological Complete Response to Neoadjuvant Chemotherapy in Breast Cancer Using DCE-MRI and InceptionV3
Authors: Pattanayak Satyabrata , Singh Tripty , Kumar Rishabh 
Published: September 2024

Abstract

Background: Neoadjuvant therapy is crucial for breast cancer patients seeking to retain the breast by reducing the tumor size for conserving surgery. This treatment aims to achieve a pathologic complete response (pCR), eradicating cancer cells entirely and reducing the risk of recurrence. The study’s objective is to devise a predictive approach for identifying patients achieving pCR through neoadjuvant therapy, based on radiomic features extracted from MR images by leveraging the InceptionV3 model with advanced validation techniques.
Methods: In our study, we gathered data from 364 unique Patient IDs sourced from the-SPY 2 MRI database with the goal of classifying pCR (pathological complete response). Our research introduced three key areas of novelty.Firstly, we explored the extraction of advanced features such as region centroid, Entropy, Sphericity, and more. These features provided deeper insights into the characteristics of the MRI data and enhanced the discriminative power of our classification model.Secondly, we applied these extracted features to the InceptionV3 (GoogleNet) model. To optimize the model’s performance, we experimented with different combinations of loss functions, optimizer functions, and activation functions. This thorough exploration allowed us to identify the most effective configuration for the given task.Lastly, our classification results were subjected 1 to validation using advanced techniques such as Matthews Correlation Coefficient, Cohen’s Kappa, and Jaccard Index. These evaluation metrics provided a robust assessment of the model’s performance and ensured the reliability of our findings.
Results: The successful combination of advanced feature extraction, utilization of the InceptionV3 model with tailored hyperparameters, and thorough validation using cutting-edge techniques significantly enhanced the accuracy and reliability of our pCR classification study. By adopting a collabo-rative approach that involved both radiologists and the computer-aided system, we achieved superior predictive performance for pCR, as evi-denced by the impressive values obtained for the area under the curve (AUC) at 0.98.
Conclusion: Overall, the combination of advanced feature extraction, lever-aging the InceptionV3 model with customized hyperparameters, and rigorous validation using state-of-the-art techniques contributed to the accuracy and credibility of our pCR classification study.
Topical psoriasis therapy: An innovative classic
Authors: Kruglova L. S. , Shatokhina E. A. 
Published: September 2024

Introduction. Psoriasis affects various aspects of the quality of life of patients, causing psycho-emotional stress, anxiety and depression, therefore treatment of patients in terms of long-term control is the main goal of therapy.Aim. To study the effectiveness of drugs containing calcipotriol and betamethasone in the treatment of plaque psoriasis of smooth skin, scalp, palmoplantar mild to moderate severity.Material and methods. We observed 59 patients and 32 patients with moderate plaque psoriasis (PASI > 10 and < 20 points). In the groups, patients were randomized into subgroups with mild and moderate severity of psoriasis.Results. In patients with mild and moderate plaque psoriasis, after 4 weeks there was a decrease in the PASI index by 89.1% and 64.1%, respectively, the DLQI index decreased by 53.8% and 61.9%, the sPGA index by 71.8% and 64.9% for mild and moderate degrees, respectively. In patients with mild and moderate palmoplantar psoriasis, after 4 weeks there was a decrease in the PPASI index by 64.2% and 62.1%, respectively, the DLQI index decreased by 56.4% and 56.7%, and the sPGA index by 63.4 % and 66.7% for mild and moderate degrees, respectively. In patients with mild to moderate psoriasis of the scalp, after 4 weeks there was a decrease in the PSSI index by 75.1% and 72.6%, the DLQI index decreased by 64.6% and 69.6%, and the sPGA index by 62.3% and 67.6% for mild and moderate degrees, respectively.Conclusion. A 52-week follow-up demonstrated the effectiveness and safety of therapy with combination drugs (Daivobet® and Xamiol®) in patients with plaque psoriasis, including scalp psoriasis and palmoplantar psoriasis. It also showed a pronounced positive effect on the quality of life of patients, including in terms of long-term control of the disease.
Polysaccharide-based Edible Film Coatings for Preservation and Packaging of Food
Authors: Sanjaykumar Gupta Swati , Malviya Rishabha , Sundaram Sonali , Sridhar Sathvik Belagodu , Singh Deependra Pratap 
Published: September 2024

Abstract:
A lot of interest has been shown in edible films and coatings made from homo- and heteropolysaccharides
as environmentally friendly substitutes for food packaging and preservation. This
review delves into the various uses, characteristics, and working principles that underpin these biopolymer-
based materials' ability to prolong food products' shelf lives and preserve their quality.
Clarified are the structure-function correlations of a variety of polysaccharides, such as starch, chitosan,
cellulose, and their blends, emphasizing their potential as barrier coverings against microbial,
oxygen, and moisture contamination. Additionally, the addition of bioactive substances and nanomaterials
to these films is covered, with a focus on how these materials improve the films' mechanical,
antioxidant, and antibacterial qualities. The paper also discusses future options for research and
case studies of these biopolymer-based systems. All things considered, the application of edible films
and coatings based on homo- and heteropolysaccharide biopolymers offers viable options for environmentally
friendly food packaging that preserves food quality and safety while lowering environmental
impact. The need for this review arises from the growing demand for sustainable, environment-
friendly alternatives to traditional plastic packaging.
Recent Progress of Chemical Components and Biological Activities of Licorice Roots
Authors: Li Le , Yong Jianping , Lu Canzhong , Tian Danian 
Published: September 2024

Abstract:
Licorice root is one kind of traditional Chinese Medicine, which has a long history of
clinical use and has been widely considered by Chinese and foreign scholars. Many works on the
chemical constituents (including flavonoids, triterpene, polysaccharides, alkaloids, and amino acids,
etc.) together with their biological activities (including anti-oxidation, anti-tumor, enhancing the
body's immunity, anti-fatigue, regulating the digestive system, lowering blood sugar, decreasing
blood lipids and so on) have been achieved. In this mini-review, we comprehensively reviewed the
recent progress of its constituents and biological activities by searching through CNKI, PubMed,
Web of Science, Scopus, and Google Scholar databases to provide a comprehensive reference for the
researchers.
Performance of ChatGPT in the In-Training Examination for Anesthesiology and Pain Medicine Residents in South Korea: Observational Study
Authors: Yoon Soo-Hyuk , Oh Seok Kyeong , Lim Byung Gun , Lee Ho-Jin 
Published: September 2024

Background
ChatGPT has been tested in health care, including the US Medical Licensing Examination and specialty exams, showing near-passing results. Its performance in the field of anesthesiology has been assessed using English board examination questions; however, its effectiveness in Korea remains unexplored.

Objective
This study investigated the problem-solving performance of ChatGPT in the fields of anesthesiology and pain medicine in the Korean language context, highlighted advancements in artificial intelligence (AI), and explored its potential applications in medical education.

Methods
We investigated the performance (number of correct answers/number of questions) of GPT-4, GPT-3.5, and CLOVA X in the fields of anesthesiology and pain medicine, using in-training examinations that have been administered to Korean anesthesiology residents over the past 5 years, with an annual composition of 100 questions. Questions containing images, diagrams, or photographs were excluded from the analysis. Furthermore, to assess the performance differences of the GPT across different languages, we conducted a comparative analysis of the GPT-4’s problem-solving proficiency using both the original Korean texts and their English translations.

Results
A total of 398 questions were analyzed. GPT-4 (67.8%) demonstrated a significantly better overall performance than GPT-3.5 (37.2%) and CLOVA-X (36.7%). However, GPT-3.5 and CLOVA X did not show significant differences in their overall performance. Additionally, the GPT-4 showed superior performance on questions translated into English, indicating a language processing discrepancy (English: 75.4% vs Korean: 67.8%; difference 7.5%; 95% CI 3.1%-11.9%; P=.001).

Conclusions
This study underscores the potential of AI tools, such as ChatGPT, in medical education and practice but emphasizes the need for cautious application and further refinement, especially in non-English medical contexts. The findings suggest that although AI advancements are promising, they require careful evaluation and development to ensure acceptable performance across diverse linguistic and professional settings.
Homestay Hosting Dynamics and Refugee Well-Being: Scoping Review
Authors: Al-Hamad Areej , Yasin Yasin Mohammad , Metersky Kateryna , Guruge Sepali , Jung Grace , Mahsud Khadija 
Published: September 2024

Background
Homestay accommodations aim to support a smoother transition for refugees; yet, the intricate nature of relationships between refugees and their hosting families can make this process complex, which, in turn, can affect their health and well-being. It is crucial to grasp the experiences of both refugees and their host families in order to foster effective settlement, integration, and well-being.

Objective
The purpose of this scoping review is to explore the dynamics of homestay or hosting with a focus on understanding the experiences of both refugees and their hosting families to identify gaps in the literature and propose directions for future research.

Methods
We used the Joanna Briggs Institute methodology and followed the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews) checklist to guide this scoping review. Searches were conducted in MEDLINE via EBSCO, Scopus via OVID, CINAHL, SOCIndex, Web of Science Core Collection, ProQuest Dissertations and Theses, the SciELO Citation Index, and APA PsycInfo. Literature written in English and published from 2011 to 2024 that focused on homestay hosting contexts for refugees was included.

Results
The results of this review illuminate the multifaceted and dynamic nature of homestay hosting for refugees. The findings include motivations and barriers for homestay hosting, factors influencing host-refugee relations, and psychological and social outcomes of homestay hosting.

Conclusions
The results of this scoping review demonstrated the need for tailored support for refugees to improve homestay programs for the benefit of both refugees and host families and highlighted the need of more inclusive, supportive, and effective strategies for the hosting, resettlement, and integration of refugees.

International Registered Report Identifier (IRRID)
RR2-10.2196/56242
Effect of Dietary Supplementation of Arjuna (Terminalia arjuna) Bark and Sahjan (Moringa oleifera) Leaf Powder on the Egg Quality and Egg Composition of Uttara Chicken
Authors: Sharma A. , Kumar A. , Sharma R. K. , Singh B. , Mondal B. C. , Prakash O. , Gangwar S. , Sarma G. 
Published: September 2024

This study was conducted during February, 2022 to February, 2023 to evaluate effect of dietary supplementation of Terminalia arjuna bark and Moringa oleifera leaf powder on external and internal egg quality parameters and egg composition of Uttara chicken. Day old chicks (n=72) were distributed into six treatment groups, having three replicates of 4 birds each. Birds were fed T0 (control diet), T1 (Basal diet+1% Arjuna bark powder+1% Sahjan leaf powder), T2 (Basal diet+1% Arjuna+2% Sahjan), T3 (Basal diet+1% Arjuna+3% Sahjan), T4 (Basal diet+1% Arjuna+4% Sahjan) and T5 (Basal diet+1% Arjuna+5% Sahjan). Eggs from the T3 group consistently exhibited higher (p<0.01) egg weight, thicker egg shell, greater egg shell weight, and improved albumen quality, as evidenced by higher albumen height, albumen index, and Haugh unit values. Yolk weight and yolk color score were also improved (p<0.01) in treatment groups, notably T2 and T3 as compared to control group. The eggs from the T3 group had significantly higher (p<0.05) crude protein content and lower yolk cholesterol levels compared to other groups. There was no significant difference in moisture (%) and ether extract (%) content of egg. Thus, it may be concluded that Arjuna bark @ 1% and Sahjan leaf @ 3% powder have positively influenced egg quality and egg composition of Uttara chicken indicating its potential as a beneficial dietary supplement in poultry diet.
No toda la inteligencia es artificial: viejas y nuevas formas de precarización en la economía digital
Authors: Hualde Alfaro Alfredo 
Published: September 2024

El presente artículo se propone indagar en los principales rasgos del trabajo y el empleo en la era digital, teniendo en cuenta los posibles efectos de las tecnologías digitales en el mercado laboral y el panorama disruptivo que se vislumbra con la rápida introducción de las herramientas ligadas a la Inteligencia Artificial. El texto se centra en primer lugar en los análisis que surgen en los países desarrollados, con la intención de examinar el tema a la luz de las características estructurales de los mercados de trabajo latinoamericanos y de la implantación progresiva del trabajo en plataformas físicas. El análisis de los empleos digitales pone de manifiesto nuevos rasgos de precariedad en ausencia de sindicatos, debilidad de las organizaciones laborales y acciones insuficientes de los gobiernos para regular los empleos mencionados.
Trabajo, familia y negociaciones cotidianas: el pluriempleo en enfermería y la organización de las responsabilidades del hogar
Authors: Malleville Sofía 
Published: September 2024

En este trabajo indagaremos en las implicancias que tiene el pluriempleo en enfermería para la organización de las responsabilidades de cuidado dentro del hogar. Los hallazgos aquí presentados se desprenden de una investigación doctoral en donde analizamos la multidimensionalidad del trabajo de cuidado en enfermería a partir de indagar en las experiencias de trabajadoras/es de instituciones de salud privadas de La Plata durante el período 2018-2021. En este artículo, nos serviremos de las entrevistas realizadas en el marco de la pandemia de COVID-19 a enfermeras mujeres que tienen dos empleos y son responsables del cuidado de hijos/as pequeños/as. Argumentaremos que las trabajadoras despliegan una serie de negociaciones cotidianas para gestionar las exigencias que experimentan producto de tener dos empleos y ser responsable de las tareas de cuidado al interior del hogar. Asimismo, señalaremos que esas múltiples demandas se intensificaron durante la emergencia sanitaria.
Scientific Justification оf Typical Projects оf Forest Crops in Various Natural Areas оf the Republic оf Kalmykia
Authors: Турчин Т.Я. , Банникова О.А. , Поповичев В.В. 
Published: September 2024

Проанализированы причины низкой лесистости Республики Калмыкии (0,2% территории). Основными факторами, влияющими на гибель создаваемых насаждений, являются жесткие лесорастительные условия и технологии лесовосстановления, которые не обеспечивают требуемую приживаемость и сохранность лесных культур. Повысить лесистость можно при тщательном планировании лесовосстановления и лесоразведения. Анализ применяемых в республике технологий и операционных приемов при воспроизводстве лесных насаждений показывает, что они не всегда соответствуют лесорастительным условиям и требуют совершенствования. Представлено научное обоснование типовых проектов лесных культур, которые различаются в зависимости от природной зоны, почвенных условий, в том числе засоления почвы. При их составлении особое внимание уделяли обработке почвы, густоте посадки (не менее 4,0 тыс. шт./га), ассортименту древесных пород, количеству и кратности агротехнических уходов.
The reasons for the low forest cover of the Republic of Kalmykia (0.2% of the territory) are analyzed. The main factors determining the death of established plantations are harsh forest conditions and applied reforestation technologies, which do not provide the required rooting and safety of forest crops. An increase in this indicator is possible with careful planning of reforestation and afforestation. The analysis of technologies and operational techniques used in the republic in the production of forest plantations does not always meet the requirements predetermined by forest conditions and requires improvement. The article presents a scientific justification of typical projects of forest crops, which vary depending on the natural zone, soil conditions, including salinity. During their development, special attention was paid to soil cultivation, planting density (at least 4.0 thousand units / ha), the range of tree species, the number and frequency of agrotechnical care.
Analysis of Errors in Estimation of the Main Taxation Indices of the First Cycle of the State Forest Inventory
Authors: Малышева Н.В. , Филипчук А.Н. , Золина Т.А. , Селезнев А.А. 
Published: September 2024

На основе данных постоянных пробных площадей (ППП) государственной инвентаризации лесов (ГИЛ) первого цикла выполнены расчеты фактической погрешности определения общего запаса древесины по Российской Федерации в целом, лесным районам и субъектам РФ. Сравнение фактических значений погрешности определения запаса древесины при доверительной вероятности 0,95 с нормативными (целевыми) значениями, установленными при планировании первого цикла ГИЛ, показало, что по стране в целом и лесным районам фактические значения оказались меньше плановых (целевых). Ранговый коэффициент корреляции целевой и фактической погрешностей +0,95. Для субъектов Российской Федерации фактические значения ошибки оценки общего запаса древесины при доверительной вероятности 0,95 больше плановых значений. Ранговый коэффициент корреляции целевой и фактической погрешностей равен -0,79. Представлены результаты статистического анализа погрешностей определения общего запаса древесины, запаса, площади и возраста основных лесообразующих пород. Показано, что по результатам ГИЛ первого цикла основные таксационные показатели оценены с высокой точностью при уровне статистической надежности 95%, приемлемой для целей стратегического планирования в масштабе страны и подготовки международной отчетности Российской Федерации по лесам.
Calculations of the actual error of the total growing stock volume for forests of the Russian Federation as a whole, forest regions and administrative units have been carried out based on permanent sample plots (PSP) data of the first cycle of the state forest inventory (SFI). Comparison of the actual errors of the total growing stock volume at a confidence level of 0.95 with the normative (target) errors established during the first SFI cycle planning showed that actual values were less than the planned (target) values for the country as a whole and the forest regions. The rank correlation coefficient of the target and actual errors is +0.95. The actual errors of the total growing stock volume at a confidence level of 0.95 are greater than the target errors for the administrative units of the Russian Federation. The rank correlation coefficient of the target and actual errors is -0.79. The results of statistical analysis of errors in estimation of the total growing stock volume, and growing stock volume, areas and age of the main forest species are presented. It is shown that, based on the results of the first SFI cycle, the main taxation indicators are evaluated with high accuracy at the 95% statistical reliability, which is acceptable for strategic planning at the national level and for international reporting on the forests of the Russian Federation.