bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera
Authors: Fan Meihua , Sun Xue , Xu Nianjun , Liao Zhi , Li Yahe , Wang Jianxin , Fan Yingping , Cui Dalian , Li Peng , Miao Zengliang 
Published: September 2017

AbstractTo investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified. At mRNA level, the up-regulated genes involved in antioxidant activity were thioredoxin,peroxiredoxin,FeSOD, glutathione peroxidase, partion catalase and MnSOD. The down-regulated genes were ascorbate peroxidase, glutathione S-transferase, catalase and MnSOD. In addition, the DEGs involved in plant signal transduction pathway (such as auxin response factors, BRI1 and JAZ) were down-regulated. At protein level, the up-regulated proteins involved in carbon fixation and the down-regulated protein mainly were polyubiquitin, ascorbate peroxidase. The expression of Ca2+-binding protein, heat shock protein and photosynthesis-related proteins, EDS1 were also significantly regulated both at mRNA and protein level. The results indicated that SA alleviated the high-temperature stimulus through partion antioxidant related proteins up-regulated, JA signal pathway enchanced, Ca2+-binding proteins, photosynthesis-related proteins significantly changed, antioxidant enzyme activities increased and photosynthesis index changed.
MicroRNA Profiling Reveals Distinct Profiles for Tissue-Derived and Cultured Endothelial Cells
Authors: Kuosmanen Suvi M. , Kansanen Emilia , Sihvola Virve , Levonen Anna-Liisa 
Published: September 2017

AbstractEndothelial plasticity enables the cells to switch their phenotype according to the surrounding vascular microenvironment. MicroRNAs (miRNAs) are small noncoding RNAs that control endothelial plasticity. The objective of this study was to investigate the differences in miRNA profiles of tissue-derived cells and cultured endothelial cells. To this end, miRNA expression was profiled from freshly isolated tissue-derived human vascular endothelial cells and endothelial cells cultured until cellular senescence using miRNA sequencing. In addition, the data was searched for putative novel endothelial miRNAs and miRNA isoforms. The data analysis revealed a striking change in endothelial miRNA profile as the cells adapted from tissue to cell culture environment and the overall miRNA expression decreased significantly in cultured compared to tissue-derived endothelial cells. In addition to changes in mechanosensitive miRNA expression, alterations in senescence-associated and endothelial-to-mesenchymal-transition-associated miRNAs were observed in aging cells. Collectively, the data illustrates the adaptability of endothelial cell miRNA expression that mirrors prevailing cellular environment.
Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation
Authors: Bhadouria Jyoti , Singh Ajit Pal , Mehra Poonam , Verma Lokesh , Srivastawa Rishi , Parida Swarup K. , Giri Jitender 
Published: September 2017

AbstractPurple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (Cicer arietnum L.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of CaPAPs under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the CaPAPs were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying CaPAPs with seed traits revealed significant association of low Pi inducible CaPAP7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.
Interleukin-22 level is negatively correlated with neutrophil recruitment in the lungs in a Pseudomonas aeruginosa pneumonia model
Authors: Broquet Alexis , Jacqueline Cédric , Davieau Marion , Besbes Anissa , Roquilly Antoine , Martin Jérôme , Caillon Jocelyne , Dumoutier Laure , Renauld Jean-Christophe , Heslan Michèle , Josien Régis , Asehnoune Karim 
Published: September 2017

AbstractPseudomonas aeruginosa is a major threat for immune-compromised patients. Bacterial pneumonia can induce uncontrolled and massive neutrophil recruitment ultimately leading to acute respiratory distress syndrome and epithelium damage. Interleukin-22 plays a central role in the protection of the epithelium. In this study, we aimed to evaluate the role of interleukin-22 and its soluble receptor IL-22BP in an acute Pseudomonas aeruginosa pneumonia model in mice. In this model, we noted a transient increase of IL-22 during Pseudomonas aeruginosa challenge. Using an antibody-based approach, we demonstrated that IL-22 neutralisation led to increased susceptibility to infection and to lung damage correlated with an increase in neutrophil accumulation in the lungs. On the contrary, rIL-22 administration or IL-22BP neutralisation led to a decrease in mouse susceptibility and lung damage associated with a decrease in neutrophil accumulation. This study demonstrated that the IL-22/IL-22BP system plays a major role during Pseudomonas aeruginosa pneumonia by moderating neutrophil accumulation in the lungs that ultimately leads to epithelium protection.
Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage
Authors: Liu Yuanwang , Chang Huiqing , Li Zhaojun , Feng Yao , Cheng Dengmiao , Xue Jianming 
Published: September 2017

AbstractGentamicin, a broad spectrum antibiotic of the aminoglycoside class, is widely used for disease prevention of human beings as well as animals. Nowadays the environmental issue caused by the disposal of wastes containing gentamicin attracts increasing attention. In this study, a gentamicin degrading bacterial consortia named AMQD4, including Providencia vermicola, Brevundimonas diminuta, Alcaligenes sp. and Acinetobacter, was isolated from biosolids produced during gentamicin production for the removal of gentamicin in the environment. The component and structure of gentamicin have a great influence on its degradation and gentamicin C1a and gentamicin C2a were more prone to being degraded. AMQD4 could maintain relatively high gentamicin removal efficiency under a wide range of pH, especially in an alkaline condition. In addition, AMQD4 could remove 56.8% and 47.7% of gentamicin in unsterilized and sterilized sewage in a lab-scale experiment, respectively. And among the isolates in AMQD4, Brevundimonas diminuta BZC3 performed the highest gentamicin degradation about 50%. It was speculated that aac3iia was the gentamicin degradation gene and the main degradation product was 3′-acetylgentamicin. Our results suggest that AMQD4 and Brevundimonas diminuta BZC3 could be important candidates to the list of superior microbes for bioremediation of antibiotic pollution.
Daytime REM sleep affects emotional experience but not decision choices in moral dilemmas
Authors: Cellini Nicola , Lotto Lorella , Pletti Carolina , Sarlo Michela 
Published: September 2017

AbstractMoral decision-making depends on the interaction between automatic emotional responses and rational cognitive control. A natural emotional regulator state seems to be sleep, in particular rapid eye movement (REM) sleep. We tested the impact of daytime sleep, either with or without REM, on moral decision. Sixty participants were presented with 12 sacrificial (6 Footbridge- and 6 Trolley-type) and 8 everyday-type moral dilemmas at 9 AM and at 5 PM. In sacrificial dilemmas, participants had to decide whether or not to kill one person to save more people (utilitarian choice), and to judge how morally acceptable the proposed choice was. In everyday-type dilemmas, participants had to decide whether to endorse moral violations involving dishonest behavior. At 12 PM, 40 participants took a 120-min nap (17 with REM and 23 with NREM only) while 20 participants remained awake. Mixed-model analysis revealed that participants judged the utilitarian choice as less morally acceptable in the afternoon, irrespective of sleep. We also observed a negative association between theta activity during REM and increased self-rated unpleasantness during moral decisions. Nevertheless, moral decision did not change across the day and between groups. These results suggest that although both time and REM sleep may affect the evaluation of a moral situation, these factors did not ultimately impact the individual moral choices.
Monitoring storage induced changes in the platelet proteome employing label free quantitative mass spectrometry
Authors: Rijkers Maaike , van den Eshof Bart L. , van der Meer Pieter F. , van Alphen Floris P. J. , de Korte Dirk , Leebeek Frank W. G. , Meijer Alexander B. , Voorberg Jan , Jansen A. J. Gerard 
Published: September 2017

AbstractShelf life of platelet concentrates is limited to 5–7 days due to loss of platelet function during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify changes in the platelet proteome during storage. In total 2501 proteins were accurately quantified in 3 biological replicates on at least 1 of the 7 different time-points analyzed. Significant changes in levels of 21 proteins were observed over time. Gene ontology enrichment analysis of these proteins revealed that the majority of this set was involved in platelet degranulation, secretion and regulated exocytosis. Twelve of these proteins have been shown to reside in α-granules. Upon prolonged storage (13–16 days) elevated levels of α-2-macroglobulin, glycogenin and Ig μ chain C region were identified. Taken together this study identifies novel markers for monitoring of the PSL that may potentially also be used for the detection of “young” and “old” platelets in the circulation.
Multi-focal sequencing of a diffuse intrinsic pontine glioma establishes PTEN loss as an early event
Authors: Koschmann Carl , Farooqui Zishaan , Kasaian Katayoon , Cao Xuhong , Zamler Daniel , Stallard Stefanie , Venneti Sriram , Hervey-Jumper Shawn , Garton Hugh , Muraszko Karin , Franchi Luigi , Robertson Patricia L. , Leonard Marcia , Opipari Valerie , Castro Maria G. , Lowenstein Pedro R. , Chinnaiyan Arul , Mody Rajen 
Published: September 2017

AbstractImproved molecular understanding is needed for rational treatment of diffuse intrinsic pontine gliomas (DIPG). Here, using multi-focal paired tumor and germline exome DNA and RNA sequencing, we uncovered phosphatase and tensin homolog (PTEN) loss as a clonal mutation in the case of a 6-year-old boy with a diffuse intrinsic pontine glioma, and incorporated copy number alteration analyses to provide a more detailed understanding of clonal evolution in diffuse intrinsic pontine gliomas. As well, using the PedcBioPortal, we found alterations in PTEN in 16 of 326 (4.9%) cases of pediatric high-grade glioma (3 of 154 (1.9%) brainstem) for which full sequencing data was available. Our data strengthens the association with PTEN loss in diffuse intrinsic pontine gliomas and provides further argument for the inclusion of PTEN in future targeted sequencing panels for pediatric diffuse intrinsic pontine gliomas and for the development and optimization of mTOR/PI3K inhibitors with optimal central nervous system penetration.
Prediction of a room-temperature eight-coordinate two-dimensional topological insulator: penta-RuS4 monolayer
Authors: Yuan Shijun , Zhou Qionghua , Wu Qisheng , Zhang Yun , Chen Qian , Hou Jing-Min , Wang Jinlan 
Published: September 2017

AbstractWe predict a stable eight-coordinate two-dimensional RuS4 monolayer, with a trilayer S–Ru–S and pentagonal rings tiling configuration by first-principles calculations. This monolayer exhibits unique anisotropic quadratic energy dispersion with two Dirac points emerging at the high-symmetric Γ point. When the spin-orbit coupling is included, a large nontrivial energy gap (70 meV) appears near the Fermi level. The topological nature of RuS4 monolayer is also confirmed by the nontrivial Z2 invariant and gapless edge states. A four-band tight-binding model is further proposed, which reveals that the topological states arise from the indirect interaction of the Ru-dxz and dyz orbitals.
Wafer-scale two-dimensional ferromagnetic Fe3GeTe2 thin films grown by molecular beam epitaxy
Authors: Liu Shanshan , Yuan Xiang , Zou Yichao , Sheng Yu , Huang Ce , Zhang Enze , Ling Jiwei , Liu Yanwen , Wang Weiyi , Zhang Cheng , Zou Jin , Wang Kaiyou , Xiu Faxian 
Published: September 2017

AbstractRecently, layered two-dimensional ferromagnetic materials (2D FMs) have attracted a great deal of interest for developing low-dimensional magnetic and spintronic devices. Mechanically exfoliated 2D FMs were discovered to possess ferromagnetism down to monolayer. It is therefore of great importance to investigate the distinct magnetic properties at low dimensionality. Here, we report the wafer-scale growth of 2D ferromagnetic thin films of Fe3GeTe2 via molecular beam epitaxy, and their exotic magnetic properties can be manipulated via the Fe composition and the interface coupling with antiferromagnetic MnTe. A 2D layer-by-layer growth mode has been achieved by in situ reflection high-energy electron diffraction oscillations, yielding a well-defined interlayer distance of 0.82 nm along {002} surface. The magnetic easy axis is oriented along c-axis with a Curie temperature of 216.4 K. Remarkably, the Curie temperature can be enhanced when raising the Fe composition. Upon coupling with MnTe, the coercive field dramatically increases 50% from 0.65 to 0.94 Tesla. The large-scale layer-by-layer growth and controllable magnetic properties make Fe3GeTe2 a promising candidate for spintronic applications. It also opens up unprecedented opportunities to explore rich physics when coupled with other 2D superconductors and topological matters.
Propionic acid-based deep eutectic solvents: synthesis and ultra-deep oxidative desulfurization activity
Authors: Mao Chun-feng , Zhao Rong-xiang , Li Xiu-ping 
Published: September 2017

Propionic acid-based deep eutectic solvents (C3H6O2/X ZnCl2, X from 0.1 to 0.6) were synthesized by stirring a mixture of propionic acid and zinc chloride at 100 °C.
Multipolar moments of weak lensing signal around clusters
Authors: Gouin C. , Gavazzi R. , Codis S. , Pichon C. , Peirani S. , Dubois Y. 
Published: September 2017

Context. Upcoming weak lensing surveys such as Euclid will provide an unprecedented opportunity to quantify the geometry and topology of the cosmic web, in particular in the vicinity of lensing clusters.
Aims. Understanding the connectivity of the cosmic web with unbiased mass tracers, such as weak lensing, is of prime importance to probe the underlying cosmology, seek dynamical signatures of dark matter, and quantify environmental effects on galaxy formation.
Methods. Mock catalogues of galaxy clusters are extracted from the N-body PLUS simulation. For each cluster, the aperture multipolar moments of the convergence are calculated in two annuli (inside and outside the virial radius). By stacking their modulus, a statistical estimator is built to characterise the angular mass distribution around clusters. The moments are compared to predictions from perturbation theory and spherical collapse.
Results. The main weakly chromatic excess of multipolar power on large scales is understood as arising from the contraction of the primordial cosmic web driven by the growing potential well of the cluster. Besides this boost, the quadrupole prevails in the cluster (ellipsoidal) core, while at the outskirts, harmonic distortions are spread on small angular modes, and trace the non-linear sharpening of the filamentary structures. Predictions for the signal amplitude as a function of the cluster-centric distance, mass, and redshift are presented. The prospects of measuring this signal are estimated for current and future lensing data sets.
Conclusions. The Euclid mission should provide all the necessary information for studying the cosmic evolution of the connectivity of the cosmic web around lensing clusters using multipolar moments and probing unique signatures of, for example, baryons and warm dark matter.
Early Dynamics of Interleukin-6 in Cerebrospinal Fluid after Aneurysmal Subarachnoid Hemorrhage
Authors: Ďuriš Kamil , Neuman Eduard , Vybíhal Václav , Juráň Vilém , Gottwaldová Jana , Kýr Michal , Vašků Anna , Smrčka Martin 
Published: September 2017

Background Subarachnoid hemorrhage (SAH) is a severe condition associated with high mortality. Early brain injury (EBI) plays an important role in the pathophysiology of SAH, and inflammation is a major contributor to EBI. Inflammation is a widely studied topic in both experimental and clinical conditions; however, just a few clinical studies have focused primarily on the early inflammatory response after SAH, and detailed information about the association between the dynamics of early inflammatory response with main clinical characteristics is lacking. This study analyzes the early dynamics of inflammatory response after SAH and evaluates the possible associations between the markers of early inflammatory response and main clinical characteristics.
Patients and Methods A total of 47 patients with a diagnosis of aneurysmal SAH within the last 24 hours were enrolled in the study. All treatments, including treatment of aneurysm (surgery/coiling) and implantation of a drainage system (external ventricular drainage/lumbar catheter), were conducted in the same way as in other patients with this diagnosis. Blood and cerebrospinal fluid (CSF) samples were collected three times a day for 4 days. The dynamics of proinflammatory cytokines were assessed, and associations between levels of the proinflammatory cytokines interleukin (IL)-6, IL-1β, or tumor necrosis factor (TNF)α and main clinical characteristics were evaluated using linear mixed-effect models.
Results The CSF levels of IL-6 were massively increased initially after SAH (up to 72 hours) with an additional increase in later phases (after 72 hours), but there was high variability in IL-6 levels. A significant association was noted between the Glasgow Outcome Scale score and both overall levels of IL-6 (p = 0.0095) and their dynamics (p = 0.0208); the effect of the Hunt and Hess scale was borderline (p = 0.0887). No association was found between IL-6 levels and Fisher grade, modality of treatment (surgery, coiling, no treatment), and later development of cerebral vasospasm. Plasmatic levels of IL-6 increased slightly, but no significant association was found. The levels of IL-1β and TNFα were within the physiologic range in both CSF and plasma.
Conclusions Early dynamics of IL-6 in CSF are associated with a patient́s outcome. But it is difficult to use IL-6 alone for outcome prediction due to its high variability. The question is whether the dynamics of IL-6 could be used in combination with other early markers associated with brain injury. More detailed research is required to answer this question.
Robotic-Assisted Ivor Lewis Esophagectomy (RAMIE) with a Standardized Intrathoracic Circular End-to-side Stapled Anastomosis and a Team of Two (Surgeon and Assistant Only)
Authors: Hadzijusufovic Edin , Lang Hauke , Grimminger Peter 
Published: September 2017

AbstractRobotic-assisted surgery is rapidly increasing, especially in general surgery. It has been shown for years that the minimal invasive esophagectomy (MIE) is possible using a robotic system, for example, da Vinci Xi, Intuitive Surgical. In the past, most robotic esophageal resections have been performed thoracoscopically, and the anastomosis was mostly sutured at the neck. Due to the increase of usable instruments and technical progress, it is possible to perform the total abdominothoracic esophagectomy with an intrathoracic sutured anastomosis robotically. In this article, we would like to present the standardized operation technique and tricks for the robotic-assisted (da Vinci Xi) Ivor Lewis MIE (RAMIE), especially the robotic technique in combination with a standardized intrathoracic circular end-to-side stapled esophagogastric anastomosis.
Neurosonologische Diagnostik in der Akutphase des Schlaganfalls ist Merkmal einer qualifizierten Versorgung
Authors: Schlachetzki Felix , Nedelmann Max , Poppert Holger , Saur Dorothee , Harloff Andreas , Liman Jan , Reinhardt Matthias , Stolz Erwin , Kaps Manfred 
Published: September 2017

ZusammenfassungDie Untersuchung der hirnversorgenden Arterien mittels Doppler- und Duplexsonografie war bislang fester Bestandteil des bewährten Stroke-Unit-Konzepts. Dies hat sich mit der Neufassung der Schlaganfall-OPS geändert: neurosonografische Untersuchungen während der Stroke-Unit-Behandlung sind im Falle der Durchführung einer CT- bzw. MR-Angiografie nicht mehr gefordert. Nach Eintritt eines ischämischen Schlaganfalls laufen im betroffenen Gefäßsegment und den dazugehörigen Kollateralwegen komplexe, individuell unterschiedliche, hämodynamische Umstellungsprozesse ab, die für das Schicksal des Patienten entscheidend sind. Verschlüsse können persistieren, partiell oder komplett rekanalisieren, kollateral kompensiert werden oder nach Rekanalisation wieder re-okkludieren. Diese Prozesse in den ersten Stunden können mittels Ultraschall wie mit keiner anderen Methode verfolgt werden. Je früher die Untersuchung erfolgt, desto aufschlussreicher sind die Befunde, desto besser ist das pathophysiologische Verständnis und desto größer ist der individuelle Nutzen für therapeutische Entscheidungen. Vielfach werden CTA, MRA und Ultraschall in der Schlaganfallversorgung als kompetitive Methoden begriffen. In Wahrheit handelt es sich aber um komplementäre Verfahren, mit denen auf der Basis von sehr unterschiedlichen physikalischen Prinzipien Bilder erzeugt und Blutgefäße, Hirnperfusion und Blutflüsse dargestellt werden. Mit Blick auf eine spezielle klinische Fragestellung ist jeweils das eine oder andere Verfahren indiziert. Es obliegt dem klinisch verantwortlichen Neurologen hier die richtige Auswahl zu treffen und spezifische neurosonologische Kompetenz einzubringen. Ziel dieser Übersichtsarbeit ist es, Neurologinnen und Neurologen dazu zu motivieren, Neurosonologie speziell in der Akutphase des Schlaganfalls einzusetzen um sich selbst ein Bild zu machen, anstatt sich „ein Bild machen zu lassen“.