bluuebunny/crossref_metadata_2025_split huggingface dataset:
Senegenin suppresses hepatocellular carcinoma by regulating O-GlcNAcylation
Authors: Zhang Xiang , Wang Li-Qiong , Liu Zhi-Yong 
Published: September 2024

BACKGROUND
Based on current knowledge, hepatocellular carcinoma (HCC) is a condition with numerous etiologies and risk factors. However, the pathogenesis of HCC remains unclear.
AIM
To investigate the roles of senegenin and O-GlcNAcylation in the growth and metastasis of HCC.
METHODS
The levels of O-linked N-acetylglucosamine transferase (OGT) and O-GlcNAcylation in HCC cells and tissues were detected using western blot analysis. The effects of senegenin and O-GlcNAcylation on the proliferation of HCC cells were investigated in vitro using cell counting kit-8 and clonogenic assays. The potential effects of senegenin and O-GlcNAcylation on HCC metastasis were examined using the transwell migration assay. O-GlcNAcylation levels were altered via drug treatment and lentiviral infection, and western blot analysis was used to detect proteins involved in various pathways.
RESULTS
Western blot analysis revealed that OGT and O-GlcNAcylation levels were significantly elevated in HCC tissues and cells. O-GlcNAcylation levels in HCC cells were significantly altered by drug treatment and lentiviral infection. An increase in the glycosylation level was linked to enhanced proliferation, invasiveness, clonogenicity, and metastatic potential of cancer cells. O-GlcNAcylation induced by senegenin was found to slow the proliferation and migration of HCC cells. The levels of proteins involved in nuclear factor-kappa B (NF-κB) and c-Jun N-terminal kinase (JNK) pathways, which are associated with endoplasmic reticulum stress, were altered.
CONCLUSION
Senegenin lowers O-GlcNAcylation levels, decreases OGT expression, and inhibits cancer cell growth and metastasis by regulating proteins involved in NF-κB and JNK pathways.
Human bone marrow mesenchymal stem cell-derived exosomes loaded with gemcitabine inhibit pancreatic cancer cell proliferation by enhancing apoptosis
Authors: Tang Zu-Gui , Chen Tie-Mei , Lu Yi , Wang Zhe , Wang Xi-Cheng , Kong Yi 
Published: September 2024

BACKGROUND
Pancreatic cancer remains one of the most lethal malignancies, and has limited effective treatment. Gemcitabine (GEM), a chemotherapeutic agent, is commonly used for clinical treatment of pancreatic cancer, but it has characteristics of low drug delivery efficiency and significant side effects. The study tested the hypothesis that human bone marrow mesenchymal stem cell (MSC)-derived exosomes loaded with GEM (Exo-GEM) would have a higher cytotoxicity against human pancreatic cancer cells by enhancing their apoptosis.
AIM
To investigate the cytotoxicity of MSC-derived Exo-GEM against pancreatic cancer cells in vitro .
METHODS
Exosomes were isolated from MSCs and characterized by transmission electron microscopy and nanoparticle tracking analysis. Exo-GEM through electroporation, sonication, or incubation, and the loading efficiency was evaluated. The cytotoxicity of Exo-GEM or GEM alone against human pancreatic cancer Panc-1 and MiaPaca-2 cells was assessed by MTT and flow cytometry assays.
RESULTS
The isolated exosomes had an average size of 76.7 nm. The encapsulation efficacy and loading efficiency of GEM by electroporation and sonication were similar and significantly better than incubation. The cytotoxicity of Exo-GEM against pancreatic cancer cells was stronger than free GEM and treatment with 0.02 μM Exo-GEM significantly reduced the viability of both Panc-1 and MiaPaca-2 cells. Moreover, Exo-GEM enhanced the frequency of GEM-induced apoptosis in both cell lines.
CONCLUSION
Human bone marrow MSC-derived Exo-GEM have a potent cytotoxicity against human pancreatic cancer cells by enhancing their apoptosis, offering a promising drug delivery system for improving therapeutic outcomes.
Imagens entre a vida e a morte
Authors: Franco Frederico 
Published: September 2024

O presente trabalho propõe analisar como o curta-metragem “Ressurreição” (1987), de Arthur Omar, relaciona-se com a morte, através do dispositivo fotográfico. Na modernidade, o advento da fotografia sugere novas conexões entre a produção de imagens e a morte. É nesse espectro teórico, que vai de Walter Benjamin a Roland Barthes, encontrando Georges Didi-Huberman, Susan Sontag e Douglas Crimp pelo caminho, que se debruçará o presente trabalho para compreender como Arthur Omar e suas fotografias mortuárias estabelecem com a morte em si? Qual o papel do dispositivo fotográfico em si na relação aqui proposta?
A Case of Hereditary Multiple Exostoses: Role of FNAC in diagnosis
Authors: Nanda Annu , Mahapatra Qury Sabita , Trivedi Shalini 
Published: September 2024

Abstract
Hereditary Multiple Exostoses (HME) is a rare autosomal dominant bone disease. It is characterized with numerous benign osteochondromas, which grow outward from the metaphyses of long bones. In many cases it is asymptomatic but can lead to considerable number of complications, like pressure symptoms, limb deformities, and can cause psychosocial problems. Malignant transformation is rarely seen. Surgery is the main modality of treatment.This paper aims to highlight the role of FNAC in diagnosis of HME.
DNA hypermethylation of tumor suppressor genes TWIST1, GATA4, MUS81 and NTRK1 in endometrial hyperplasia
Authors:   Department of Obstetrics and Gynecology, Faculty of Medicine of the UK and FN Hradec Králové, Czech Republic, Dvořák Ondřej , Slavíčková Marcela , Laco Jan , Štěpán Martin , Čermáková Eva , Špaček Jiří 
Published: September 2024

Objective: To investigate DNA methylation of specific tumor suppressor genes in endometrial hyperplasia compared to normal endometrial tissue. File and methodology: To search for epigenetic events, methylation-specific multiplex ligation-dependent probe amplification was employed to compare the methylation status of 40 tissue samples with atypical endometrial hyperplasia, 40 tissue samples with endometrial hyperplasia without atypia, and 40 control tissue samples with a normal endometrium. Results and conclusion: Differences in DNA methylation among the groups were found in TWIST1, GATA4, MUS81, and NTRK1 genes (TWIST1: atypical hyperplasia 67.5%, benign hyperplasia 2.5%, normal endometrium 22.5%; P < 0.00001; GATA4: atypical hyperplasia 95%, benign hyperplasia 65%, normal endometrium 22.5%; P < 0.00001; MUS81: atypical hyperplasia 57.5%, benign hyperplasia 22.5%, normal endometrium 5%; P < 0.00001; NTRK1: atypical hyperplasia 65%, benign hyperplasia 27.5%, normal endometrium 10%; P < 0.00001). Higher methylation rates were observed for the tumor suppressor genes of TWIST1, GATA4, MUS81, and NTRK1 in samples with atypical endometrial hyperplasia compared to samples with normal endometrial tissue, and higher methylation rates were found in samples with atypical endometrial hyperplasia compared to samples of benign endometrial hyperplasia. DNA methylation of TWIST1, GATA4, MUS81, and NTRK1 is involved in the pathogenesis of atypical endometrial hyperplasia. Key words: methylation – TWIST1 – GATA4 – MUS81 – NTRK1 – endometrial hyperplasia – epigenetics
Effects of a structured exercise therapy on health-related quality of life in pediatric stem cell transplantation
Authors: Jung Michael Wilhelm , Schubert Patric , Stephenson Antonia , Bader Peter 
Published: September 2024

IntroductionThis randomized controlled trial investigated the effects of exercise therapy on cancer-related fatigue (CRF) and its association with health-related quality of life. The effect of exercise therapy on the quality of life of children after stem cell transplantation has not been extensively studied to date.MethodsSeventy-two children and adolescents after stem cell transplantation were randomly assigned to either an exercise therapy intervention group (IG) or a control group (CG). The children in IG received sports therapy, while the children in the CG engaged in concentration tasks, memory games, and quizzes.ResultsSignificant improvements in CRF were found in IG only after discharge from the hospital. The improved CRF correlated significantly with increased health-related quality of life. However, during the inpatient phase, there was a rapid increase in CRF initially, which was accompanied by a significant reduction in health-related quality of life.ConclusionsExercise therapy can help reduce CRF and, therefore, increase health-related quality of life after the inpatient phase.
Mind the gap: treating post-surgical scar tissue in post-mastectomy patients – a randomized clinical trial
Authors: Górecki Maciej , Naczk Mariusz , Doś Janusz , Anioła Beata , Majchrzycki Marian , Marszałek Sławomir 
Published: September 2024

IntroductionPost-mastectomy physiotherapy typically includes massage, exercise, and stretching, but often fails to treat post-surgical scar tissue, a significant contributor to long-term disability. This study aimed to compare conventional therapy with myofascial manual therapy for treating post-surgical scar tissue in post-mastectomy patients and to determine the effectiveness of these treatments.MethodsThe study was an experimental controlled study with pre- and post-testing of two parallel groups. Forty-eight women experiencing shoulder and/or upper torso impairment following mastectomy were randomly allocated to either a control group (<i>n</i> = 21) or a myofascial therapy group (<i>n</i> = 27). Conventional physiotherapy involving exercise and massage or myofascial therapy was used. Pre- and post-therapy measurements included muscle length, pain intensity, range of motion (ROM), and scar mobility.ResultsMyofascial therapy was superior to conventional physiotherapy in reducing the percentage of patients with below-normal muscle lengths at several sites. Significant decreases from baseline were observed in the treatment group for the pars clavicularis, descending trapezius, pars sternocostalis, latissimus dorsi, and levator scapulae muscles. Myofascial therapy also improved mobility in the scar area, with increases of 3 mm relative to the xiphoid process and 4 mm relative to the coracoid.ConclusionsOur study indicates that myofascial interventions improve upper limb ROM and muscle elasticity in the upper limb girdle of post-mastectomy patients. These findings provide empirical evidence supporting the benefits of myofascial intervention over conventional physiotherapy in the treatment of post-surgical scar tissue in this patient group.
Reliability and concurrent validity of the bubble inclinometer for visual estimation of straight leg raise in asymptomatic individuals
Authors: Somprasong Sirikarn , Sakulsriprasert Prasert , Vachalathiti Roongtiwa , Kingcha Pathaimas , Janyathitipath Thanachaporn 
Published: September 2024

IntroductionThe straight leg raise (SLR) test is commonly used to investigate neurodynamic problems or hamstring muscle length. Visual estimation can be used to identify the degrees of hip flexion during the SLR test. However, intra-tester and inter-tester reliability, as well as concurrent validity, await formal investigation.MethodsThis study was an experimental study. Two testers, a novice and an experienced physical therapist, measured hip flexion angles during the SLR test using visual estimation for two sessions and the bubble inclinometer method during the first session in 31 asymptomatic participants, in random order. Intra-tester reliability (intraclass correlation coefficient, ICC<sub>3,3</sub>), inter-tester reliability (ICC<sub>2,3</sub>), concurrent validity (Pearson correlation with bubble inclinometer), and measurement error was represented as standard error of measurement (SEM) and minimal detectable change (MDC), were calculated.ResultsThe concurrent validity and intra-tester reliability of visual estimation for both testers were good (ICC<sub>3,3</sub> = 0.885 with 95% confidence interval = 0.775–0.943, <i>p</i> < 0.001) and excellent (ICC<sub>3,3</sub> = 0.904 with 95% confidence interval = 0.810–0.952, <i>p</i> < 0.001), respectively. Inter-tester reliability of visual estimation was poor (ICC<sub>2,3</sub> = 0.373 with 95% confidence interval = 0.027–0.639, <i>p</i> = 0.018).ConclusionsAlthough the concurrent validity of visual estimation with the bubble inclinometer was good, and intra-tester reliability was excellent, the inter-tester reliability was poor. Therefore, caution should be exercised if more than one tester is involved in visual estimation.
Gesunder Boden, guter Ertrag
Authors: Vetter Bernhard 
Published: September 2024

Frankfurt a.M. Ist der Boden gesund, stimmt auch der Ertrag – oder andersherum: Wenn der Ertrag hoch ist, deutet das auf einen gesunden Boden hin. Das ist eine Erkenntnis aus dem Hofgespräch Boden.
Metabolitos secundarios en cuatro cultivares de Plumeria sp. relaciones con el órgano y el ambiente
Authors: Valera Rosario E. , Maciel de Sousa Norberto M. , Sanabria María Elena 
Published: September 2024

Los metabolitos secundarios (MS) son moléculas sintetizadas en bajas concentraciones por las plantas que desempeñan un rol importante en la adaptación de las mismas al ambiente, y que depende de las condiciones internas y externas. En la familia Apocynaceae se ubica Plumeria sp., una planta cultivada como ornamental, que constituye una fuente de compuestos biológicamente activos, algunos de uso como fármacos. Se realizó un análisis fitoquímico mediante cromatografía de capa fina, en extractos etanólicos obtenidos de órganos aéreos y subterráneos de P. pudica “Biotipo V”, P. pudica 'Bridal White', P. obtusa 'Singapure White' y el híbrido P. rubra x P. obtusa 'Mele Pa Bowman', colectados en época de lluvia, transición y sequía, a partir de plantas cultivadas en el Posgrado de Agronomía, Universidad Centroccidental Lisandro Alvarado, en Venezuela. La mayor concentración de alcaloides para todos los materiales se determinó en el tallo, en sequía, y además en transición, para P. pudica y P. obtusa.; del mismo modo, los fenoles fueron más abundantes en raíz y en época de sequía. En el caso de los flavonoides, los valores más altos de concentración se obtuvieron en el tallo y en sequía, para casi todos los materiales, excepto para el híbrido, en el cual ocurrió en las hojas en esa misma época y en las inflorescencias, en transición. Las saponinas se determinaron, en todos los cultivares, a muy bajas concentraciones. La época de colecta fue un factor de importancia en la síntesis de los MS y concentración de éstos, las diferencias podrían relacionarse a factores de estrés, que influyen en la bioproducción.
Efficiency of handmade attractants in fruit fly control
Authors: García-Mendoza Pedro J. , Morales Valles Pedro A. , Pérez-Almeida Iris , Taramona Ruíz Luís A. , Marín-Rodríguez Carlos A. 
Published: September 2024

Fruit flies (Diptera: Tephritidae) are considered the main pests of fruit crops worldwide, and using traps and attractants are useful tools for their effective monitoring and control. The study's main objective was to evaluate the capacity to capture fruit flies with non-commercially produced attractants and use the Lin and Binns method to evaluate their performance. Five food attractants were evaluated to capture the genera Anastrepha and Ceratitis. The study was carried out in five municipalities of Táchira State, Venezuela, during two semesters, using a completely randomized design with four replications. The experimental unit was represented by a JD EuGo 97 trap, baited with 300 mL of the respective attractant solution. The attractants did not perform equally in all environments, while no variation was observed in the level of trap capture between the two semesters studied. Anastrepha fraterculus was the only species collected at all altitudinal levels, suggesting an adequate capacity to adapt to different environments. The commercial product PedGo plus was the most effective attractant for fly trapping, and it may be an appropriate alternative for use by fruit growers in the control of this pest. Although it was the most efficient attractant in most of the environments studied, molasses + urea proved to be an alternative to replace PedGo plus and Nulure.
Penerapan Kombinasi Metode Simple Additive Weighting dan Technique for Order Preference by Similarity to Ideal Solution dalam Pemilihan Dosen Pembimbing Skripsi
Authors: Joko Kuswanto  
Published: September 2024

Penetapan atau pemilihan dosen pemblimbling skripsi di Program Studi Informatika Universitas BLaturaja bliasanya dilakukan atas usulan dari mahasiswa. Pemilihan terseblut umumnya dilakukan secara manual dan blelum dilakukan dengan sistem yang terkomputerisasi. Selain itu, penetapan dosen pemblimbling skripsi terkadang mengesampingkan jumlah blimblingan yang mengakiblatkan kurang seimblangnya jumlah blimblingan dari setiap dosen dan terkadang tidak sesuai dengan kompetensi dosen. Melihat kondisi terseblut, maka diperlukan sebluah yang dapat mendukung dalam pengamblilan keputusan untuk pemilihan dosen pemblimbling skripsi. Penelitian ini blertujuan menerapkan komblinasi metode SAW dengan TOPSIS dalam pemilihan Dosen Pemblimbling Skripsi. Metode SAW digunakan terleblih dahulu untuk menghitung nilai pembloblotan pada setiap kriteria di tiap altenatif dan metode TOPSIS seblagai tahapan proses akhir untuk melakukan proses perangkingan terhadap alternatif. Dari hasil perhitungan dengan menerapkan komblinasi metode SAW dan TOPSIS didapatkan 3 nilai tertinggi seblesar 0,7338, 0.5805, dan 0.5302 pada Alternatif 5, 6, dan 3. BLerdasarkan hasil perhitungan dapat disimpulkan blahwa penerapan komblinasi metode SAW dan TOPSIS dapat diimplementasikan ke dalam sistem pemilihan dosen pemblimbling skripsi.
O TRABALHO EM EDUCAÇÃO INFANTIL REALIZADO NO CMEI - LUÍZ ANTÔNIO DE PONTES RAMOS NO MUNICÍPIO DE CAMARAGIBE - PE
Authors: Soares Rosana Maria , Gusmão Diogenes José 
Published: September 2024

Este artigo tem o objetivo de abordar o modelo de Educação Infantil oferecido no CMEI- Luíz Antônio de Pontes Ramos, que faz parte da rede municipal da cidade de camaragibe em Pernambuco. Esta pesquisa foi pensada com o objetivo de explicar a abordagem metodológica utilizada na educação infantil do CMEI e foi realizada com o intuito de apresentar aspectos que se destacam na abordagem metodologica para o ensino - aprendizagem, com o objetivo de gerar em seus leitores uma reflexão sobre sua prática com as crianças de creche e pré-escola. Traremos a concepção desta prática, na maneira de como a criança é vista e compreendida em sua totalidade. Serão apresentados os principais aspectos da prática utilizada no CMEI, explanando alguns pontos fundamentais para o entendimento, sendo alguns deles, como são organizados os ambientes de aprendizagem, qual o papel do professor e a importância da documentação pedagógica na educação infantil. A intenção deste artigo é evidenciar o que podemos nos apropriar da aprendizagem utilizada no CMEI e assim tornar nossa prática na educação infantil significativa para as crianças e para nós enquanto professores, nesse sentido a reflexão do papel docente é importante para a superação de práticas descontextualizadas e que não valorizam as experiências das crianças e suas vivências.
An improved estimate of inorganic iodine emissions from the ocean using a coupled surface microlayer box model
Authors: Pound Ryan J. , Brown Lucy V. , Evans Mat J. , Carpenter Lucy J. 
Published: September 2024

Abstract. Iodine at the ocean's surface impacts climate and health by removing ozone (O3) from the troposphere both directly via ozone deposition to seawater and indirectly via the formation of iodine gases that are released into the atmosphere. Here we present a new box model of the ocean surface microlayer that couples oceanic O3 dry deposition to inorganic chemistry to predict inorganic iodine emissions. This model builds on the previous work of Carpenter et al. (2013), improving both chemical and physical processes. This new box model predicts iodide depletion in the top few micrometres of the ocean surface due to rapid chemical loss to ozone competing with replenishment from underlying water. From this box model, we produce parameterized equations for HOI and I2 emissions, which are implemented into the global chemical transport model GEOS-Chem along with an updated sea surface iodide climatology. Compared to the previous model, inorganic iodine emissions from some tropical waters decrease by as much as half, while higher-latitude emissions increase by a factor of ≫10. With these large local changes, global total inorganic iodine emissions increased by ∼49 % (2.99 to 4.48 Tg) compared to the previous parameterization. This results in a negligible change in average tropospheric OH (<0.2 %) and tropospheric methane lifetime (<0.2 %). The annual mean tropospheric O3 burden decreases (−1.5 % to 325 Tg); however, higher-latitude surface O3 concentrations decrease by as much as 20 %.
Godmother-Governesses and Biracial Cinderellas: The Use of Fairy Tales in Dinah Mulock Craik’s The Half-Caste
Authors: Mummert Hannah 
Published: September 2024

Abstract
In Dinah Mulock Craik’s short and largely unacknowledged novella, The Half-Caste (1851), the half-Indian heroine, Zillah La Poer, achieves what seems impossible for a young woman of color in mid-century imperial Britain, rising from an uneducated and ill-treated biracial ward to a rich heiress-turned-bride, all under the watchful guidance of the novella’s narrator and main character, governess Cassandra Pryor. Though Craik’s novella has resurfaced in recent years with the help of a Broadview edition in 2016, the scholarly work and historical material in the introduction and appendixes do not highlight the fairy-tale inspirations and obvious Cinderella motifs in Craik’s text. Craik’s novella is, at its core, a Cinderella story, as Cassandra becomes a nonmagical fairy godmother tasked with transforming Zillah into a respected British citizen. As such, this article seeks to bring attention to Craik’s little-known novella by situating it in a larger tradition of Victorian Cinderella stories, highlighting the way it creates an idealized image of civilized harmony between non-white colonized people and white British citizens via governess-led education.
Elisabeth Jerichau-Baumann, Constantinople 1869–70: The Harem
Authors: Kuehn Julia , Possen David D. 
Published: September 2024

Abstract
Polish-born, trained in Germany, with a studio in Rome and a second home in Denmark due to her marriage to sculptor and academician Adolf Jerichau, Elisabeth Jerichau-Baumann (1818–81) was a true nineteenth-century cosmopolite. She painted Europe’s elite and counted Princess Alexandra, Hans Christian Andersen, Henrik Ibsen, the Grimm brothers, and Charles Dickens among her well-wishers. She was invited by Queen Victoria to Buckingham Palace, and her portrait of Alexandra remains in the Royal Collection to this day. Her travelogue Brogede Rejsebilleder (Motley Images of Travel;1881) is centered around two journeys to “the Orient,” undertaken in 1869–70 and 1874–75. While these journeys to Constantinople and Smyrna constitute almost half of the travelogue, other chapters describe journeys to Athens, Cairo, St. Petersburg, Capri, and the Alps. The present chapters, translated by David Possen and introduced by Julia Kuehn, are the second part of Jerichau-Baumann’s record of the first of two journeys to Constantinople, in 1869–70. The painter vividly describes a visit to a Constantinople harem. Jerichau-Baumann’s temporary friendship with the young Princess Nazlı Hanım would lead to a number of paintings by Jerichau-Baumann now considered emblematic of and unique in (female) Orientalist art. Nazlı became a well-known literary salon hostess and arts supporter in Istanbul, Paris, Cairo, and Tunis, as well as an important figure in the Ottoman Empire’s political reform, modernization, and cross-cultural endeavors.