bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ancient hybridization and genomic stabilization in a swordtail fish
Authors: Schumer Molly , Cui Rongfeng , Powell Daniel L. , Rosenthal Gil G. , Andolfatto Peter 
Published: March 2016

AbstractA rapidly increasing body of work is revealing that the genomes of distinct species often exhibit hybrid ancestry, presumably due to postspeciation hybridization between closely related species. Despite the growing number of documented cases, we still know relatively little about how genomes evolve and stabilize following hybridization, and to what extent hybridization is functionally relevant. Here, we examine the case of Xiphophorus nezahualcoyotl, a teleost fish whose genome exhibits significant hybrid ancestry. We show that hybridization was relatively ancient and is unlikely to be ongoing. Strikingly, the genome of X. nezahualcoyotl has largely stabilized following hybridization, distinguishing it from examples such as human–Neanderthal hybridization. Hybridization‐derived regions are remarkably distinct from other regions of the genome, tending to be enriched in genomic regions with reduced constraint. These results suggest that selection has played a role in removing hybrid ancestry from certain functionally important regions. Combined with findings in other systems, our results raise many questions about the process of genomic stabilization and the role of selection in shaping patterns of hybrid ancestry in the genome.
Anatomy of the national myth: archetypes and narrative in the study of nationalism
Authors: Morden Michael 
Published: March 2016

AbstractThis paper argues that recognising types of underlying narrative form which repeatedly occur across cases is critical to the study of nationalism. It proposes a method borrowed from the literary theory of Northrop Frye – archetypal criticism – for identifying the four basic forms of emotional architecture that characterise the myths of particular nations: tragic, romantic, comic and satiric. The study of nationalism has long acknowledged the importance of narrative in political behaviour. But consideration of how distinct types of narratives affect specific emotions is missing. The ‘narrative turn’ in the social sciences, which has responded to instrumentalist scepticism, has thus far focused on the cognitive functions of narrative. That is, how narrative influences the acquisition and interpretation of information and how stories are used to construct or reinforce a collective understanding of events. The undertheorised dimension of narrative in nationalism relates to the emotional structures embedded within narrative. This is where this paper makes its contribution.
Arrhythmic Burden and Ambulatory Monitoring of Pediatric Patients with Cardiomyopathy
Authors: CZOSEK RICHARD J. , JEFFERIES JOHN L. , KHOURY PHILIP R. , ANDERSON JEFFREY B. , WILMOT IVAN , KNILANS TIMOTHY K. , SPAR DAVID S. 
Published: March 2016

BackgroundPediatric patients with cardiomyopathy (CM) are at risk for sudden cardiac death (SCD), likely driven by arrhythmic etiologies.ObjectivesDescribe arrhythmia burden and Holter utility in pediatric CM including: hypertrophic CM (HCM), dilated CM (DCM), and restrictive CM (RCM).MethodsRetrospective cohort study of patients <21 years with CM. Patient demographics, arrhythmic history, and genetic status were reviewed including outcomes of death, aborted SCD, and device shocks. Holter findings were analyzed over the prior 5 years including clinically significant findings and resulting changes to management. Analysis for the composite outcomes of death, aborted SCD, and appropriate shock were performed using logistic regression with backward elimination.ResultsOne hundred and forty‐six patients were included: 83 HCM, 54 DCM, and nine RCM (mean 13 ±
6 years). A total of 23% of patients had defibrillators. There were six deaths (two SCD), four patients with appropriate device therapies, and four aborted SCD episodes. In total, 305 Holter monitors were reviewed. Six Holters had significant findings, all nonsustained ventricular tachycardia. Two Holters resulted in changes in management, both defibrillator implantations. Twelve patients had one or more of the conditions defining the composite outcome. Using logistic regression, clinical history of ventricular arrhythmia, frequent premature ventricular complexes, and CM type were included as potential independent predictors in the final model and clinical ventricular arrhythmia and RCM disease were associated with the composite outcome.ConclusionsSCD and device therapies were relatively rare. Routine Holter screening rarely demonstrated significant findings or changed clinical care. Clinical history of ventricular arrhythmia was associated with poor clinical outcome.
Developmental Changes in Neonatal and Infant Skin Structures During the First 6 Months: In Vivo Observation
Authors: Miyauchi Yuki , Shimaoka Yayoi , Fujimura Tsutomu , Koike Yumi , Yatabe Michio , Nishikawa Masakatsu , Hayashi Masaru , Sugata Keiichi , Moriwaki Shigeru , Hatamochi Atsushi 
Published: March 2016

AbstractBackground/ObjectivesDevelopmental changes of structures in neonatal and infant skin have not been well characterized. The purpose of this study was to clarify changes in skin structures during neonatal and infant growth in vivo.MethodsFifteen healthy, full‐term neonates (seven girls, eight boys) were studied. The measurements were performed 4 to 7 days (neonate) and 1, 3, and 6 months after birth on the buttock, upper thigh, and ventral forearm skin using a confocal laser scanning microscope. Developmental changes in dermoepidermal junction structures, stratum corneum thickness, epidermal thickness, and microvascular development were investigated.ResultsA significant decrease in stratum corneum thickness was observed over the 3 months after birth. Dermal papillae were not observed in neonatal skin but were observed gradually over the next 3 months. Epidermal thickness, determined from the skin surface to the bottom of the epidermal layer, increased significantly from 4 to 7 days to 1 month of age, indicating the formation of dermal papillae and rete ridges. Complicated microvascular structures were observed in neonatal skin but disappeared gradually and were observed only at the dermal papillae at 3 months of age.ConclusionsOur results reveal that infant skin is in a developmental stage structurally up to 3 months of age, paralleling skin functional and developmental maturation.
Dental stories for children with autism
Authors: Marion Ian W , Nelson Travis M. , Sheller Barbara , McKinney Christy M. , Scott JoAnna M. 
Published: March 2016

ABSTRACTPurposeTo investigate caregivers' preference regarding dental stories to prepare children with autism for dental visits.MethodsCaregivers of children with autism were allowed use of dental stories available via different media (paper, tablet computer, computer) and image types (comics or drawings, photographs, video). Caregivers completed pre‐ and postintervention surveys. Fisher's exact tests were used to determine associations between predictive factors and preferences.ResultsForty initial and 16 follow‐up surveys were completed. Subjects were primarily male (85%). Mean child age was 6.7 years. Nine (64%) caregivers found the dental story useful for themselves and their child. Two (14%) caregivers found the aid only helpful for themselves. Preferred media type was associated with language understanding (p = .038) and home media preference (p = .002).ConclusionsPractitioners should consider using dental stories to help prepare families and children for dental visits. Individual preferences for dental stories vary; using prior history can aid in selection.
Together in Need: Relational Selfhood, Vulnerability to Harm, and Enriching Attachments
Authors: Wolfe Katharine 
Published: March 2016

AbstractConnections between one's own welfare and that of others abound if we pause to look for them, although philosophical theories of selfhood have only very recently begun to incorporate these connections. This essay draws on recent work on need to argue that one of the strongest expressions of these connections is to be found in the relational needs that they can generate. While paying heed to needs that arise from the relational nature of selfhood at large, this essay pays particular attention to what I call “transpersonal needs”: needs that occur when one's experience of the needs of others gives rise to certain needs of one's own. I argue that the best criterion for defining need is vulnerability to harm, but this does not mean that having a need is something that is purely harmful. Having certain needs can also enrich one's life. Further, while every need entails a corresponding vulnerability to harm, some of these potential harms are more detrimental to one's welfare than others, with some relational needs standing among those that can result in the greatest harm if unanswered.
A Person‐Oriented Analysis of Social Withdrawal in Chinese Children
Authors: Coplan Robert J. , Liu Junsheng , Ooi Laura L. , Chen Xinyin , Li Dan , Ding Xuechen 
Published: March 2016

AbstractThe goal of this study was to compare the socio‐emotional and academic adjustment of different subtypes of socially withdrawn (shy, unsociable, avoidant) school‐age children in mainland China. Participants were N = 1344 children ages 10–12 years from public elementary schools in Shanghai, People's Republic of China. Multi‐source assessment included: child self‐reports of social withdrawal subtypes and internalizing difficulties (e.g., depression, social anxiety); peer nominations of children's peer relations (e.g., peer victimization, peer preference); and teacher ratings of children's school adjustment (e.g., academic success, internalizing problems). Results from person‐oriented analyses indicated that socially avoidant (i.e., shy‐unsociable) children reported the most pervasive internalizing difficulties compared to other groups. However, in contrast to findings among Western samples, unsociable children were as likely to have peer and academic difficulties as their shy and socially avoidant peers. Findings are discussed in terms of the implications of different subtypes of social withdrawal among children in collectivistic societies such as China.
Reading Formations of Subjectivity: From Discourse to Psyche
Authors: Brock Maria 
Published: March 2016

AbstractThis article represents a critical overview of strategies to examine subjectivity in discourse, highlighting a series of methodological approaches, which seek to manage the tension between discourse studies' focus on social and cultural structures, and psychoanalysis' interest in unconscious motivations. One aim is to trouble the supposed opposition between discourse analysis and the psychosocial approach and to regard the latter as a possible extension of insights established by the former. It is argued here that psychosocial readings in general, and Lacanian approaches more specifically, offer a cautious, nuanced way of introducing psychoanalytic ideas into the analysis of texts. The first part of this article offers examples of discourse analytic approaches, which have explicitly sought to incorporate psychoanalytic notions, followed by a discussion of Lacanian discourse analysis – a method shaped directly by this psychoanalytic school's concern with language. The article concludes with a series of methodological injunctions for conducting a psychosocial form of textual analysis.
Late Mesoproterozoic rifting in Arctic Canada during Rodinia assembly: impactogens, trans‐continental far‐field stress and zinc mineralisation
Authors: Turner Elizabeth C. , Long Darrel G. F. , Rainbird Robert H. , Petrus Joseph A. , Rayner Nicole M. 
Published: March 2016

AbstractThis paper challenges the conventional interpretation of a major, economically important Mesoproterozoic intracratonic rift system as a group of aulacogens, proposing instead that they are rifts that developed in response to far‐field stress caused by continent–continent collision (impactogens) during supercontinent assembly. The tectonostratigraphic evolution of the Bylot basins (Arctic Canada) records dramatic alternations between extensional and compressional stress regimes, precluding an aulacogen interpretation and favouring an impactogen interpretation. New geochronological data (U–Th–Pb whole‐rock depositional age; detrital zircon signatures) provide a record of impactogens that developed in Laurentia's interior as a result of Grenvillian (~1.1 Ga) far‐field stress during assembly of the supercontinent Rodinia. Formation of the world‐class Nanisivik zinc deposit in one of the rifts is temporally associated with the Grenvillian orogeny, and consequently represents both (i) a rare exception to the global pattern of few ore deposits forming during supercontinent episodes, and (ii) a hitherto unrecognised tectonic setting for carbonate‐hosted zinc deposits.
Modeling Co‐evolution of Speech and Biology
Authors: de Boer Bart 
Published: March 2016

AbstractTwo computer simulations are investigated that model interaction of cultural evolution of language and biological evolution of adaptations to language. Both are agent‐based models in which a population of agents imitates each other using realistic vowels. The agents evolve under selective pressure for good imitation. In one model, the evolution of the vocal tract is modeled; in the other, a cognitive mechanism for perceiving speech accurately is modeled. In both cases, biological adaptations to using and learning speech evolve, even though the system of speech sounds itself changes at a more rapid time scale than biological evolution. However, the fact that the available acoustic space is used maximally (a self‐organized result of cultural evolution) is constant, and therefore biological evolution does have a stable target. This work shows that when cultural and biological traits are continuous, their co‐evolution may lead to cognitive adaptations that are strong enough to detect empirically.
Endogenous mycotic endophthalmitis in a dog with candiduria and Evans syndrome
Authors: Enders Andrew , van der Woerdt Alexandra , Donovan Taryn 
Published: March 2016

AbstractThis report describes the clinical presentation, diagnosis, histologic lesions, and outcome of endogenous mycotic endophthalmitis secondary to candiduria in a three‐year‐old female spayed Dachshund. The dog was being treated for Evans syndrome for one month prior to being diagnosed with candiduria and fibrinous uveitis OS. The left eye was enucleated due to secondary glaucoma, and the fungal urinary tract infection was treated successfully. Uveitis developed in the contralateral eye with relapse of the urinary tract infection in the following weeks. The right eye was medically managed until secondary glaucoma developed and was subsequently enucleated. Histopathology of both eyes showed evidence of endophthalmitis with intralesional fungal organisms, consistent with Candida spp. Ocular candidiasis is rare in dogs. To the authors' knowledge, this is the first report of endogenous mycotic endophthalmitis with concurrent candiduria in a dog.
A novel property of gold nanoparticles: Free radical generation under microwave irradiation
Authors: Paudel Nava Raj , Shvydka Diana , Parsai E. Ishmael 
Published: March 2016

Purpose:Gold nanoparticles (GNPs) are known to be effective mediators in microwave hyperthermia. Interaction with an electromagnetic field, large surface to volume ratio, and size quantization of nanoparticles (NPs) can lead to increased cell killing beyond pure heating effects. The purpose of this study is to explore the possibility of free radical generation by GNPs in aqueous media when they are exposed to a microwave field.Methods:A number of samples with 500 mM 5,5‐dimethyl‐1‐pyrroline N‐oxide (DMPO) in 20 ppm GNP colloidal suspensions were scanned with an electron paramagnetic resonance (EPR)/electron spin resonance spectrometer to generate and detect free radicals. A fixed (9.68 GHz) frequency microwave from the spectrometer has served for both generation and detection of radicals. EPR spectra obtained as first derivatives of intensity with the spectrometer were double integrated to get the free radical signal intensities. Power dependence of radical intensity was studied by applying various levels of microwave power (12.5, 49.7, and 125 mW) while keeping all other scan parameters the same. Free radical signal intensities from initial and final scans, acquired at the same power levels, were compared.Results:Hydroxyl radical (OH⋅) signal was found to be generated due to the exposure of GNP–DMPO colloidal samples to a microwave field. Intensity of OH⋅ signal thus generated at 12.5 mW microwave power for 2.8 min was close to the intensity of OH⋅ signal obtained from a water–DMPO sample exposed to 1.5 Gy ionizing radiation dose. For repeated scans, higher OH⋅ intensities were observed in the final scan for higher power levels applied between the initial and the final scans. Final intensities were higher also for a shorter time interval between the initial and the final scans.Conclusions:Our results observed for the first time demonstrate that GNPs generate OH⋅ radicals in aqueous media when they are exposed to a microwave field. If OH⋅ radicals can be generated close to deoxyribonucleic acid of cells by proper localization of NPs, NP‐aided microwave hyperthermia can yield cell killing via both elevated temperature and free radical generation.
Spiking neurons can discover predictive features by aggregate-label learning
Authors: Gütig Robert 
Published: March 2016

Credit assignment in the brain
To discover relevant clues for survival, an organism must bridge the gap between the short time periods when a clue occurs and the potentially long waiting times after which feedback arrives. This so-called temporal credit-assignment problem is also a major challenge in machine learning. Gütig developed a representation of the responses of spiking neurons, whose derivative defines the direction along which a neuron's response changes most rapidly. By using a learning rule that follows this development, the temporal credit-assignment problem can be solved by training a neuron to match its number of output spikes to the number of clues. The same learning rule endows unsupervised neural networks with powerful learning capabilities.

Science
, this issue p.
10.1126/science.aab4113
Human Immunodeficiency Virus Diagnostic Testing: 30 Years of Evolution
Authors: Alexander Thomas S. 
Published: March 2016

ABSTRACT
A concern during the early AIDS epidemic was the lack of a test to identify individuals who carried the virus. The first HIV antibody test, developed in 1985, was designed to screen blood products, not to diagnose AIDS. The first-generation assays detected IgG antibody and became positive 6 to 12 weeks postinfection. False-positive results occurred; thus, a two-test algorithm was developed using a Western blot or immunofluorescence test as a confirmatory procedure. The second-generation HIV test added recombinant antigens, and the third-generation HIV tests included IgM detection, reducing the test-negative window to approximately 3 weeks postinfection. Fourth- and fifth-generation HIV assays added p24 antigen detection to the screening assay, reducing the test-negative window to 11 to 14 days. A new algorithm addressed the fourth-generation assay's ability to detect both antibody and antigen and yet not differentiate between them. The fifth-generation HIV assay provides separate antigen and antibody results and will require yet another algorithm. HIV infection may now be detected approximately 2 weeks postexposure, with a reduced number of false-positive results.
Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
Authors: Crisci Elisa , Ellegård Rada , Nyström Sofia , Rondahl Elin , Serrander Lena , Bergström Tomas , Sjöwall Christopher , Eriksson Kristina , Larsson Marie 
Published: March 2016

ABSTRACTHerpes simplex virus 2 (HSV-2) is one of the most common sexually transmitted infections globally, with a very high prevalence in many countries. During HSV-2 infection, viral particles become coated with complement proteins and antibodies, both present in genital fluids, which could influence the activation of immune responses. In genital mucosa, the primary target cells for HSV-2 infection are epithelial cells, but resident immune cells, such as dendritic cells (DCs), are also infected. DCs are the activators of the ensuing immune responses directed against HSV-2, and the aim of this study was to examine the effects opsonization of HSV-2, either with complement alone or with complement and antibodies, had on the infection of immature DCs and their ability to mount inflammatory and antiviral responses. Complement opsonization of HSV-2 enhanced both the direct infection of immature DCs and their production of new infectious viral particles. The enhanced infection required activation of the complement cascade and functional complement receptor 3. Furthermore, HSV-2 infection of DCs required endocytosis of viral particles and their delivery into an acid endosomal compartment. The presence of complement in combination with HSV-1- or HSV-2-specific antibodies more or less abolished HSV-2 infection of DCs. Our results clearly demonstrate the importance of studying HSV-2 infection under conditions that ensuein vivo, i.e., conditions under which the virions are covered in complement fragments and complement fragments and antibodies, as these shape the infection and the subsequent immune response and need to be further elucidated.IMPORTANCEDuring HSV-2 infection, viral particles should become coated with complement proteins and antibodies, both present in genital fluids, which could influence the activation of the immune responses. The dendritic cells are activators of the immune responses directed against HSV-2, and the aim of this study was to examine the effects of complement alone or complement and antibodies on HSV-2 infection of dendritic cells and their ability to mount inflammatory and antiviral responses. Our results demonstrate that the presence of antibodies and complement in the genital environment can influence HSV-2 infection underin vitroconditions that reflect thein vivosituation. We believe that our findings are highly relevant for the understanding of HSV-2 pathogenesis.