bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pre‐Selection Test to Identify High Responder Donor Goats
Authors: Balaro MFA , Brandão FZ , Maia ALRS , Souza‐Fabjan JMG , Cueto MI , Gibbons AE , Fonseca JF 
Published: April 2016

ContentsThe aim of the study was to evaluate the feasibility of pre‐selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long‐term progestogen treatment. Fourteen days later, a second progestogen protocol associated with a multiple‐dose follicle stimulation hormone (FSH) treatment (5 IU/kg of FSH, in six decreasing doses between days 4 to 6 of the protocol) was administered. Transrectal ultrasound was used to assess the follicular status at the beginning of superovulatory treatments, at the oestrous onset and on the seventh day of the oestrous cycle for counting corpora lutea (CL). A significant lower number of CL was obtained in eCG‐treated in comparision with FSH‐treated does (p < 0.05). A quartic regression was able to explain the relationship between the number of CL in response to both treatments (r2=0.50; p < 0.05). Seventy per cent (14 of 20) of does maintained the same ovulatory response (high or low) after treatments. The Kappa (κ = 0.40; p < 0.05) and Spearman (rs = 0.39; p = 0.08) coefficients were able to show a relationship between treatments. Regarding the follicular status, there is a significant relationship between the number of small follicles (r = 0.71; r2=0.47; p < 0.01) and total follicles (r = 0.60; p < 0.01) at eCG and first FSH dose with the number of CL. Moreover, it was found a negative relationship between the presence of large follicles and the number of CL in response to eCG treatment (r = −0.44; p < 0.05), but not from FSH (p > 0.05). In conclusion, the screening test with eCG has the potential to identify Saanen does that will better respond to the superovulatory protocol with FSH. In addition, it highlighted the importance of an ultrasound evaluation prior to the beginning of superovulatory treatments with FSH to characterize the follicular status and identify the potential donors of high ovulatory response in MOET programmes in goats.
The Coping Long Term with Active Suicide Program: Description and Pilot Data
Authors: Miller Ivan W. , Gaudiano Brandon A. , Weinstock Lauren M. 
Published: April 2016

A description of and pilot data for a new adjunctive intervention designed to reduce suicidal behavior among high‐risk individuals are presented. The Coping Long Term with Active Suicide Program (CLASP) has three major components: (1) three individual, in‐person meetings, (2) one significant other/family meeting, and (3) 11 brief (15–30 minute) phone contacts with the patient and his/her significant other. Open trial and pilot randomized trial data provide preliminary evidence that CLASP is a feasible, acceptable, and efficacious intervention to reduce suicidal behavior.
Connecting Eating Pathology with Risk for Engaging in Suicidal Behavior: The Mediating Role of Experiential Avoidance
Authors: Skinner Kayla D. , Rojas Sasha M. , Veilleux Jennifer C. 
Published: April 2016

Individuals with eating pathology, particularly those with diagnosed eating disorders, are at high risk for suicide. It is less clear whether undiagnosed eating pathology and subsyndromal eating disorders carry the same risk and, if so, what mechanisms may explain why higher levels of eating pathology yield greater risk for engaging in suicidal behaviors. The indirect relationship between disordered eating and risk for suicidal behaviors via facets of experiential avoidance was tested using a multiple‐mediator model. The model was tested using bootstrapping estimates of indirect effects in a sample of 218 noncollege student adults (Mage = 32.33, 66.1% women) with a history of suicidal attempt and/or history of nonsuicidal self‐injury (NSSI). Results revealed that disordered eating indirectly predicted risk for suicidal behaviors, distress aversion (i.e., negative attitudes or dislike of distress), and procrastination (i.e., delaying engagement with distressing activities). Results suggest that targeting experiential avoidance and helping those who have a history of engaging in suicidal behaviors and/or NSSI develop regulation strategies to use during times of distress may be of utmost importance for treatment and prevention of eating pathology.
Effects of velocity loss during resistance training on athletic performance, strength gains and muscle adaptations
Authors: Pareja‐Blanco F. , Rodríguez‐Rosell D. , Sánchez‐Medina L. , Sanchis‐Moysi J. , Dorado C. , Mora‐Custodio R. , Yáñez‐García J. M. , Morales‐Alamo D. , Pérez‐Suárez I. , Calbet J. A. L. , González‐Badillo J. J. 
Published: April 2016

We compared the effects of two resistance training (RT) programs only differing in the repetition velocity loss allowed in each set: 20% (VL20) vs 40% (VL40) on muscle structural and functional adaptations. Twenty‐two young males were randomly assigned to a VL20 (n = 12) or VL40 (n = 10) group. Subjects followed an 8‐week velocity‐based RT program using the squat exercise while monitoring repetition velocity. Pre‐ and post‐training assessments included: magnetic resonance imaging, vastus lateralis biopsies for muscle cross‐sectional area (CSA) and fiber type analyses, one‐repetition maximum strength and full load‐velocity squat profile, countermovement jump (CMJ), and 20‐m sprint running. VL20 resulted in similar squat strength gains than VL40 and greater improvements in CMJ (9.5% vs 3.5%, P < 0.05), despite VL20 performing 40% fewer repetitions. Although both groups increased mean fiber CSA and whole quadriceps muscle volume, VL40 training elicited a greater hypertrophy of vastus lateralis and intermedius than VL20. Training resulted in a reduction of myosin heavy chain IIX percentage in VL40, whereas it was preserved in VL20. In conclusion, the progressive accumulation of muscle fatigue as indicated by a more pronounced repetition velocity loss appears as an important variable in the configuration of the resistance exercise stimulus as it influences functional and structural neuromuscular adaptations.
The prevalence and clinical significance of premature ventricular beats in the athlete
Authors: D'Ascenzi F. , Zorzi A. , Alvino F. , Bonifazi M. , Corrado D. , Mondillo S. 
Published: April 2016

Adolescents and adults with cardiovascular disease who are engaged in sports activity have an increased risk of sudden cardiac death (SCD) that is three times greater than that of their non‐athletic counterparts. Sport acts as a trigger for cardiac arrest in the presence of underlying cardiovascular diseases predisposing to life‐threatening ventricular arrhythmias. Frequent and complex premature ventricular beats (PVBs) detected during the cardiovascular screening of the athletic population may be a sign of an underlying cardiovascular disease at risk of SCD, but are also often recorded in trained athletes without cardiovascular abnormalities. Thus, the interpretation of PVBs could represent a clinical dilemma, particularly in the athlete. However, while some characteristics of PVBs can be considered common and benign, others occur uncommonly in the athletic population and raise the suspicion of an underlying cardiovascular disease. This review discusses the prevalence and clinical significance of PVBs in the athlete, with a focus on exercise‐induced PVBs, on the analysis of PVB's morphology at 12‐lead ECG, and on the morphological substrates identified by imaging techniques. The implications on eligibility for competitive sports participation are also discussed, according to the relevance of PVB detection for disqualifying athletes from competitions.
Vertical stiffness asymmetries during drop jumping are related to ankle stiffness asymmetries
Authors: Maloney S. J. , Richards J. , Nixon D. G. D. , Harvey L. J. , Fletcher I. M. 
Published: April 2016

Asymmetry in vertical stiffness has been associated with increased injury incidence and impaired performance. The determinants of vertical stiffness asymmetry have not been previously investigated. Eighteen healthy men performed three unilateral drop jumps during which vertical stiffness and joint stiffness of the ankle and knee were calculated. Reactive strength index was also determined during the jumps using the ratio of flight time to ground contact time. “Moderate” differences in vertical stiffness (t17 = 5.49; P < 0.001), “small” differences in center of mass displacement (t17 = −2.19; P = 0.043), and “trivial” differences in ankle stiffness (t17 = 2.68; P = 0.016) were observed between stiff and compliant limbs. A model including ankle stiffness and reactive strength index symmetry angles explained 79% of the variance in vertical stiffness asymmetry (R2 = 0.79; P < 0.001). None of the symmetry angles were correlated to jump height or reactive strength index. Results suggest that asymmetries in ankle stiffness may play an important role in modulating vertical stiffness asymmetry in recreationally trained men.
Ctenocephalides felis and Ctenocephalides canis: introgressive hybridization?
Authors: ZURITA ANTONIO , CALLEJÓN ROCÍO , DE ROJAS MANUEL , HALAJIAN ALI , CUTILLAS CRISTINA 
Published: April 2016

AbstractIn the present work, a comparative molecular study of Ctenocephalides felis and Ctenocephalides canis isolated from dogs (Canis lupus familiaris) from different geographical regions (Spain, Iran and South Africa) was carried out. We found morphological variations in C. felis that do not correspond with molecular differences. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and 18S rRNA partial gene, and cytochrome c‐oxidase 1 (cox1) mtDNA partial gene sequences were determined to clarify the taxonomic status of these two species, and to assess interpopulation variation and interspecific sequence differences. In addition, a comparative phylogenetic study with other species of fleas using Bayesian, Maximum Parsimony and Maximum Likelihood analysis was performed. The 18S rRNA partial gene fragment was useful neither to discriminate C. canis and C. felis nor to infer phylogenetic relationships at this level, whereas ITS1 and ITS2 assessed for specific determination in the genus Ctenocephalides. The cox1 mtDNA sequences of C. felis revealed three main haplotypes and we suggest that there has been introgression of C. canis cox1 mtDNA into C. felis by Wolbachia pipientis. Based on cox1 sequences, restriction mapping identified many endonucleases that could be used to delineate different haplotypes of C. felis and to differentiate C. felis and C. canis.
The Minorca Basin: a buffer zone between the Valencia and Liguro‐Provençal Basins (NW Mediterranean Sea)
Authors: Pellen Romain , Aslanian Daniel , Rabineau Marina , Leroux Estelle , Gorini Christian , Silenziario Carmine , Blanpied Christian , Rubino Jean‐Loup 
Published: April 2016

AbstractDetailed analyses of seismic profiles and boreholes in the Valencia Basin (VB) reveal a differentiated basin, the Minorca Basin (MB), lying between the old Mesozoic Valencia Basin sensu strico (VBss) and the young Oligocene Liguro‐Provençal Basin (LPB). The relationship between these basins is shown through the correlation of four Miocene‐to‐present‐day megasequences. The Central and North Balearic Fracture Zones (CFZ and NBFZ) that border the MB represent two morphological and geodynamical thresholds that created an accommodation in steps between the three domains. Little to no horizontal Neogene movements are found for the Ibiza and Majorca Islands. In contrast, the counterclockwise movement of the Corso‐Sardinian blocks induced a counterclockwise movement of the Minorca block towards the SE along the CFZ and NBFZ, during the exhumation of lower continental crust in the LPB. This new understanding implies pure Neogene vertical subsidence in the VBss and places the AlKaPeCa northeastward of the present‐day Alboran Area.
Late sequelae of left ventricular assist device infection presenting after heart transplant
Authors: Dulanto Chiang A. , Narsana N. , Ruiz M.E. 
Published: April 2016

AbstractThe long‐term use of left ventricular assist devices (LVADs) is becoming more common among the end‐stage heart failure population. At the time of heart transplantation, most of the LVAD is removed, but some of its components might be retained. Retained LVAD prosthetic material can lead to serious infection post heart transplant. We report 4 such cases. Our goal is to highlight the importance of complete prosthetic material removal at the time of cardiac transplant to prevent late‐onset infection, especially in patients with preceding infection, but also in patients without evidence of LVAD infection prior to orthotopic heart transplantation.
Human Fumarate Hydratase Is Dual Localized by an Alternative Transcription Initiation Mechanism
Authors: Dik Ekaterina , Naamati Adi , Asraf Hadar , Lehming Norbert , Pines Ophry 
Published: April 2016

AbstractFumarate hydratase (FH, fumarase), is a tricarboxylic acid cycle enzyme localized in the mitochondrial matrix. However, a common theme, conserved from yeast to human, is the existence of a large cytosolic population of FH. FH has been shown to function as a tumor suppressor gene and is now implicated in various diseases. We have previously indicated that the cytosolic echoform of FH has a role in the DNA damage response and specifically in the response to DNA double strand breaks. In fact, recently FH has been shown to be involved in histone demethylation. Therefore, it has become important to understand the underlying mechanism of FH dual subcellular location in human cells. We revealed that in human cells, in contrast to yeast, the FH gene encodes two gene products, one containing and one lacking the mitochondrial targeting sequence. On the basis of expression of endogenous wild‐type FH and mutant FH cDNAs from plasmids, RT‐PCR, RACE to determine the 5′ termini of FH mRNAs, and mass spectrometry of FH products, we show that the mechanism of FH distribution is alternative transcription initiation from a broad promoter. This is contrary to the suggested mechanism for rat liver cells which had claimed alternative translation initiation.
Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery
Authors: Fritsch G. , Frank N. , Dmytrus J. , Frech C. , Pichler H. , Witt V. , Geyeregger R. , Scharner D. , Trbojevic D. , Zipperer E. , Printz D. , Worel N. 
Published: April 2016

Background and ObjectivesOur post‐thaw cell recovery rates differed substantially in interlaboratory comparisons of identical samples, potentially due to different temperatures during cell staining.Materials and MethodsViable CD34+ cells and leucocyte (WBC) subtypes were quantified by multiparameter single‐platform flow cytometry in leucapheresis products collected from 30 adult lymphoma and myeloma patients, and from 10 paediatric patients. After thawing, cells were prepared for analysis within 30 min between thawing and acquisition, at either 4°C or at room temperature.ResultsFor cell products cryopreserved in conventional freezing medium (10% final DMSO), viable cell recovery was clearly lower after staining at 4°C than at RT. Of all WBC subtypes analysed, CD4+ T cells showed the lowest median recovery of 4% (4°C) vs. 25% (RT), followed by CD3, CD34 and CD8 cells. The recovery was highest for CD3γδ cells with 44% (4°C) vs. 71% (RT). In the 10 samples cryopreserved in synthetic freezing medium (5% final DMSO), median recovery rates were 89% for viable CD34 (both at 4°C and RT) and 79% (4°C) vs 68% (RT) for WBC.ConclusionsThe post‐thaw environment and, potentially, the cryoprotectant impact the outcome of cell enumeration, and results from the analysis tube may not be representative of the cells infused into a patient.
A cost and clinical effectiveness analysis among moist wound healing dressings versus traditional methods in home care patients with pressure ulcers
Authors: Souliotis Kyriakos , Kalemikerakis Ioannis , Saridi Maria , Papageorgiou Manto , Kalokerinou Athena 
Published: April 2016

AbstractThe aim of the study was a cost and clinical effectiveness analysis between moist wound healing dressings and gauze in a homecare set up for the treatment of stage III and IV pressure ulcers up to complete healing. In addition, we assessed the overall economic burden on the Healthcare System. Treatment method for each patient was chosen randomly by using sealed opaque envelopes. The authors monitored the healing progress and recorded treatment costs without interfering with the treatment process. The healing progress was estimated by using surface measurement transparent films. To estimate treatment costs, the authors took into account labor costs, cost of dressings, as well the cost of other materials such as cleansing gauzes, normal saline, syringes, examination gloves, antiseptics and adhesive tape. The patient group under treatment with moist wound healing dressings consisted of 27 men and 20 women aged 75.1 ± 8,6 and had an average ulcer surface of 43.5 ± 30.70 cm2; the patient group under treatment with gauze comprised 25 men and 23 women aged 77.02 ± 8.02 and had an average ulcer surface 41.52 ± 29.41 cm2 (p = 0.25, 95% CI, Student's t test). The average healing time for the moist wound healing dressings group' was 85.56 ± 52.09 days, while 121.4 ± 52.21 days for the “gauze group” (p = 0.0001, 95% CI, Student's t test). The dressing change frequency per patient was reduced in the “moist wound healing dressings group,” 49.5 ± 29.61, compared with a dressing change frequency per patient of 222.6 ± 101.86 for the “gauze group” (p = 0.0001, 95% CI, Student's t test). The use of moist wound healing dressings had a lower total treatment cost of 1,351 € per patient compared with, the use of gauzes (3,888 €).
Phylogeny and taxonomic reassessment of pikas Ochotona pallasii and O. argentata (Mammalia, Lagomorpha)
Authors: Lissovsky Andrey A. , Yatsentyuk Svetlana P. , Ge Deyan 
Published: April 2016

We examined a cranial morphometric data set consisting of 186 specimens from the entire distribution range of Ochotona pallasii sensu lato and O. argentata, as well as 67 complete sequences of the COI gene and 28 sequences of the MGF and PRKCI introns from these and closely allied species. Our results show that the two allopatric morphologically similar taxa composing O. pallasii sensu lato – from Mongolia and adjacent territories and Kazakhstan – are paraphyletic relative to O. argentata. Genetic distances between these three taxa are larger than the intraspecific variation known for the subgenus Pika, in which the species under consideration belong; these distances are even larger than the interspecific differences among closely related species such as O. hyperborea, O. mantchurica and O. hoffmanni. Thus, the three focal taxa are recognized here as distinct species. Inspection of the type specimen of O. pallasii indicated that this specimen was not collected in Kazakhstan, has previously been theorized. The most probable place of the holotype's origin is Russian south‐eastern Altai (Chuyskaya Steppe); whatever its exact origin, it definitively originates from the ‘Mongolian’ taxon. Based on this evidence, the senior synonym for the Kazakh pika is O. opaca Argyropulo, 1930. Thus, we propose to recognize three separate species in the O. pallasii species group: O. pallasii (Mongolia and adjacent territories), O. opaca (eastern Kazakhstan) and O. argentata (Helan Shan Range, China).
Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretion
Authors: Pais Ramona , Rievaj Juraj , Meek Claire , De Costa Gayan , Jayamaha Samanthie , Alexander R. Todd , Reimann Frank , Gribble Fiona 
Published: April 2016

Key points
Arginine vasopressin (AVP) stimulates the release of enteroendocrine L‐cell derived hormones glucagon‐like peptide‐1 (GLP‐1) and peptide YY (PYY) in vitro from mouse and human colons.
This is mediated by the AVP receptor 1B, which is highly enriched in colonic L‐cells and linked to the elevation of L‐cell calcium and cAMP concentrations.
By means of Ussing chambers, we show that AVP reduced colonic anion secretion, although this was blocked by a specific neuropeptide Y receptor Y1 receptor antagonist, suggesting that L‐cell‐released PYY acts locally on the epithelium to modulate fluid balance.
In human serum samples, PYY concentrations were higher in samples with raised osmolality and copeptin (surrogate marker for AVP).
These findings describe, for the first time, the role of L‐cells in AVP regulated intestinal fluid secretion, potentially linking together hormonal control of blood volume and blood glucose levels, and thus adding to our understanding of the complex pathways involved in the gut hormonal response to different stimuli.
AbstractArginine vasopressin (AVP) regulates fluid balance and blood pressure via AVP receptor (AVPR)2 in the kidney and AVP receptor 1A in vascular smooth muscle. Its role in intestinal function has received less attention. We hypothesized that enteroendocrine L‐cells producing glucagon‐like peptide 1 (GLP‐1) and peptide YY (PYY) may be a target of AVP and contribute to the control of fluid balance. Avpr1b expression was assessed by quantitative RT‐PCR on flourescence‐activated cell sorting‐isolated L‐ and control cells and was enriched in colonic L‐cells. AVP stimulated GLP‐1 and PYY release from primary cultured murine and human colonic cells and was associated with elevated calcium and cAMP concentrations in L‐cells as measured in cultures from GLU‐Cre/ROSA26‐GCaMP3 and GLU‐Epac2camps mice. An antagonist of AVPR1B reduced AVP‐triggered hormone secretion from murine and human cells. In Ussing chambers, basolaterally applied AVP reduced colonic anion secretion and this effect was blocked by a specific neuropeptide Y receptor Y1 (NPY1R) antagonist. In human serum, PYY concentrations were higher in samples with raised osmolality or copeptin (a surrogate marker for AVP). In conclusion, we propose that AVP activates L‐cell AVPR1B, causing GLP‐1 and PYY secretion. PYY in turn reduces colonic anion secretion via epithelial NPY1R. Our data suggest L‐cells are active players in the hypothalamic control of intestinal fluid homeostasis, providing a potential link between the regulation of blood volume/pressure/osmolality and blood glucose.
Associations Between Balance and Muscle Strength, Power Performance in Male Youth Athletes of Different Maturity Status
Authors: Hammami Raouf , Chaouachi Anis , Makhlouf Issam , Granacher Urs , Behm David G. 
Published: April 2016

Balance, strength and power relationships may contain important information at various maturational stages to determine training priorities. Purpose:The objective was to examine maturity-specific relationships of static/dynamic balance with strength and power measures in young male athletes.Methods:Soccer players (N = 130) aged 10–16 were assessed with the Stork and Y balance (YBT) tests. Strength/power measures included back extensor muscle strength, standing long jump (SLJ), countermovement jump (CMJ), and 3-hop jump tests. Associations between balance with strength/power variables were calculated according to peak-height-velocity (PHV).Results:There were significant medium-large sized correlations between all balance measures with back extensor strength (r = .486–.791) and large associations with power (r = .511–.827). These correlation coefficients were significantly different between pre-PHV and circa PHV as well as pre-PHV and post-PHV with larger associations in the more mature groups. Irrespective of maturity-status, SLJ was the best strength/power predictor with the highest proportion of variance (12–47%) for balance (i.e., Stork eyes opened) and the YBT was the best balance predictor with the highest proportion of variance (43–78%) for all strength/power variables.Conclusion:The associations between balance and muscle strength/power measures in youth athletes that increase with maturity may imply transfer effects from balance to strength/power training and vice versa in youth athletes.