bluuebunny/crossref_metadata_2025_split huggingface dataset:
Access to dental services and use of toothpaste with optimal fluoride content in Peruvian children
Authors: Hadad-Arrascue Natalie , Garcés-Elías María Claudia 
Published: October 2024

BackgroundLeading international health organizations advise using toothpaste that contains at least 1,000 parts per million of fluoride, emphasizing that this practice should begin with the eruption of the first primary tooth.AimTo determine the association between access to dental services and the use of toothpaste with 1,000 ppm fluoride or more in Peruvian children under twelve years of age.Materials and methodsA cross-sectional analysis was conducted using data from the 2021 Demographic and Family Health Survey. The main variable studied was the use of toothpaste containing a fluoride concentration of 1,000 ppm or more. Additionally, the access to dental care, the time elapsed since the last dental visit, and the type of healthcare facility were analyzed. Multilevel regression was applied to examine the relationship between variables and the dependent variable, with Peru's 24 regions serving as the analytical level.ResultsAccording to the bivariate analysis, the natural region, area of residence, place of residence, wealth index, and age were associated with the use of fluoride toothpaste with 1,000 ppm or more. In the multilevel analysis, the access to dental services was not associated with the use of fluoride toothpaste with 1,000 ppm or more (p = 0.454); similarly, the time elapsed since the last dental visit also showed no association (p = 0.676), as did the type of healthcare facility (p = 0.752, p = 0.896, p = 0.983).ConclusionAccessing dental services, the time elapsed since the last dentist visit, and the location where that care was received were not associated with the use of toothpaste containing 1,000 ppm or more of fluoride among Peruvian children during the year 2021.
Comparative analysis of the bioaccumulation of bisphenol A in the blood serum and follicular fluid of women living in two areas with different environmental impacts
Authors: Raimondo Salvatore , Chiusano Maria Luisa , Gentile Mariacira , Gentile Tommaso , Cuomo Felice , Gentile Raffaella , Danza Domenico , Siani Laura , Crescenzo Claudia , Palmieri Mariangela , Iaccarino Stefania , Iaccarino Mirella , Fortunato Adriana , Liguori Francesca , Esposito Antonio , Zullo Clelia , Sosa Loredana , Sosa Laura , Ferrara Ida , Piscopo Marina , Notari Tiziana , Lacatena Raffaele , Gentile Alberto , Montano Luigi 
Published: October 2024

IntroductionBisphenol A (BPA) is a common contaminant widely used in many industrial sectors. Because of its wide use and dispersion, it can be accumulated in living human bodies through both oral assumption and nondietary routes. BPA exhibits hormone-like properties, falling under the class of endocrine disruptors; therefore, it can alter relevant physiological functions. In particular, in women, it can affect folliculogenesis and therefore reproduction, contributing not only to infertility, but also to endometriosis and premature puberty.MethodsWe conducted a multicenter study on 91 women undergoing a first in vitro fertilization (IVF) treatment in the Campania region (Southern Italy). We investigated the presence and concentration of BPA in serum and follicular fluids to assess the effects of airborne BPA contamination. The analysis was conducted on 32 women living in a low environmental impact (LEI) area, from the Sele Valley River and Cilento region, and 59 women living in a high environmental impact (HEI) area, the so-called “Land of Fires”, a highly contaminated territory widely exposed to illegal waste practices.ResultsA higher average BPA content in both blood serum and follicular fluid was revealed in the HEI group when compared with the LEI group. In addition, we revealed higher average BPA content in blood serum than in folliclular fluid in the HEI area, with opposite average content in the two fluids in the LEI zone. In addition, our results also showed a lack of correlation between BPA content in follicular and serum fluids both in the overall population and in the HEI and LEI groups, with peculiar trends in different subsets of women.ConclusionFrom our results, we revealed a heterogeneity in the distribution of BPA content between serum and follicular fluid. Further studies are needed to unravel the bioaccumulation mechanisms of BPA in highly polluted and nonpolluted areas.
Transcriptomic insights into mycorrhizal interactions with tomato root: a comparative study of short- and long-term post-inoculation responses
Authors: Abdelsattar Mohamed , Soliman Maali S. , Mohamed Rasha A. , Radwan Khaled H. , El-Mahdy Mohamed M. , Mousa Khaled H. , Khalil Shaimaa R. M. , Osman Engy , Alameldin Hussien F. , Hussein Ahmed , Hassanein Sameh E. , Abdallah Naglaa A. , Alsamman Alsamman M. , Osama Omnia 
Published: October 2024

BackgroundArbuscular mycorrhiza (AM) refers to a symbiotic association between plant roots and fungi that enhances the uptake of mineral nutrients from the soil and enables the plant to tolerate abiotic and biotic stresses. Although previously reported RNA-seq analyses have identified large numbers of AM-responsive genes in model plants, such as Solanum lycopersicum L., further studies are underway to comprehensively understand the complex interactions between plant roots and AM, especially in terms of the short- and long-term responses after inoculation.ResultsHerein, we used RNA-seq technology to obtain the transcriptomes of tomato roots inoculated with the fungus Rhizophagus irregularis at 7 and 30 days post inoculation (dpi). Of the 1,019 differentially expressed genes (DEGs) in tomato roots, 635 genes showed differential expressions between mycorrhizal and non-mycorrhizal associations at the two time points. The number of upregulated DEGs far exceeded the number of downregulated ones at 7 dpi, and this difference decreased at 30 dpi. Several notable genes were particularly involved in the plant defense, plant growth and development, ion transport, and biological processes, namely, GABAT, AGP, POD, NQO1, MT4, MTA, and AROGP3. In addition, the Kyoto encyclopedia of genes and genomes pathway enrichment analysis revealed that some of the genes were involved in different pathways, including those of ascorbic acid (AFRR, GME1, and APX), metabolism (CYP, GAPC2, and CAM2), and sterols (CYC1 and HMGR), as well as genes related to cell division and cell cycle (CDKB2 and PCNA).ConclusionThese findings provide valuable new data on AM-responsive genes in tomato roots at both short- and long-term postinoculation stages, enabling the deciphering of biological interactions between tomato roots and symbiotic fungi.
Upcycling end-of-life carbon fiber in high-performance CFRP composites by the material extrusion additive manufacturing process
Authors: Ateeq Muhammad , Nazir Aamer 
Published: October 2024

Each year, a significant amount of waste is produced from carbon fiber polymer composites at the end of its lifecycle due to extensive use across various applications. Utilizing regenerative carbon fiber as a feedstock material offers a promising and sustainable approach to additive manufacturing based on materials. This study proposes the additive manufacturing of recycled carbon fiber with a polyamide-12 polymer composite. Filaments of recycled carbon fiber-reinforced polyamide-12 (rCF-PA12) with different recycled carbon fiber contents (0%, 10%, and 15% by weight) in the polyamide-12 matrix are developed. These filaments are utilized for 3D printing of specimens by using various infill density parameters (80% and 100%) on a fused deposition modeling 3D printer. The study examined how the fiber content and infill densities influenced the flexural performance of the printed specimens. Notably, the part containing 15 wt% recycled carbon fiber (rCF) composites showed a significant improvement in flexural performance due to enhanced interface bonding and effective fiber alignment. The results indicated that reinforcing the printed part with 10% and 15 wt% recycled carbon fiber (rCF) improved the flexural properties by 49.86% and 91.75%, respectively, compared to the unreinforced printed part under the same infill density and printing parameters. The investigation demonstrates that the additive manufacturing-based technique presents a potential approach to use carbon fiber-reinforced polymers waste and manufacture high-performance engineering, economic, and environmentally friendly industrial applications with the complicated design using different polymer matrices.
Construction of frailty and risk prediction models in maintenance hemodialysis patients: a cross-sectional study
Authors: Liu Huan , Tao Mingfen , Zhang Man , Zhou Zhiqing , Ni Yang , Wang Qin , Zhang Xiang , Chi Chenru , Yang Dan , Chen Mengqi , Tao Xiubin , Zhang Ming 
Published: October 2024

ObjectiveAs the prevalence of diabetic nephropathy and hypertensive nephropathy increases with age in mainland China, the number of patients with end-stage renal disease is increasing, leading to an increase in the number of patients receiving maintenance hemodialysis. Considering the harmful effects of frailty on the health of maintenance hemodialysis patients, this study aims to identify hemodialysis patients at risk for frailty at an early stage, in order to prevent or delay the progression of frailty in the early stage, so as to prevent the adverse consequences of frailty.MethodsA total of 479 patients admitted to the blood purification centers of two grade tertiary hospitals in Anhui Province, China, using convenient sampling. The Frailty Scale, the SARC-F questionnaire, the Simplified Food Appetite Questionnaire (SNAQ) and the mini nutritional assessment short-form (MNA-SF) were used in the study. Pearson correlation analysis was used to explore the correlation among the frailty influencing factors.ResultsThe incidence of frailty was 24.0% among 479 Chinese hemodialysis patients. Gender (p &lt; 0.05), Malnutrition (p &lt; 0.001), sarcopenia (p &lt; 0.001), and feel tired after dialysis (p &lt; 0.001) were highly correlated with frailty in Chinese hemodialysis patients. Serum albumin concentration (p &lt; 0.05) was a protective factor of frailty.ConclusionThis survey shows that frailty was highly prevalent among Chinese hemodialysis patients. Medical staff and their families should make early judgments and carry out interventions on the risk of frailty.
Correlation between body mass index and gender-specific 28-day mortality in patients with sepsis: a retrospective cohort study
Authors: Li Chong , Huang Huaping , Xia Qingjie , Zhang Li 
Published: October 2024

ObjectiveTo investigate the potential correlation between body mass index (BMI) and the 28-day mortality rate among sepsis patients and the gender difference in this association.DesignThe current research was a retrospective cohort study.ParticipantsA total of 14,883 male and female cohorts of sepsis patients were included in the Medical Information Mart for Intensive Care IV (MIMIC-IV V2.2) database. Patients in each gender cohort were further classified as underweight, normal weight, overweight, or obese according to BMI and the World Health Organization (WHO) BMI categories.OutcomesThe 28-day mortality from the date of ICU hospitalization was the primary outcome measure.ResultsThe BMI and 28-day mortality exhibited an L-shaped relationship (p for nonlinearity &lt;0.001) with significant gender-specific differences. Subgroup analysis revealed different association patterns between the male and female cohorts. Specifically, BMI and mortality exhibited a U-shaped curve relationship among the males (p for nonlinearity &lt;0.001) and an L-shaped relationship among the females (p for nonlinearity = 0.045).ConclusionThis study proposes a link between extreme BMI and 28-day mortality in patients with sepsis. Underweight patients have an increased risk of mortality; however, this risk decreases in overweight and obese patients. Upon stratifying by sex, a U-shaped pattern was observed, indicating an association between BMI and 28-day mortality in males, while an L-shaped pattern emerged in females.
A rapid and reliable method for early Legionella pneumophila identification and characterization in support of the epidemiology study
Authors: Monistero Valentina , Vicari Nadia , Prati Paola , Bragoni Roldano , Gazzola Alessandra , Sala Lorenza , Maisano Antonio , Moroni Paolo , Bronzo Valerio , Luini Mario Vittorio , Castiglioni Bianca , Cremonesi Paola 
Published: October 2024

IntroductionLegionnaires’ disease is a severe pneumonia predominantly caused by Legionella pneumophila (Lp), whose major reservoirs are artificial water systems. As most human infections are caused by L. pneumophila serogroup 1 (Lp1), a reliable method for Lp distinction can be crucial for bacterial spread prevention. As the ability to withstand in environments and to cause the waterborne disease is strongly related to specific genes, the identification of virulent strains can be of great relevance to implement water environmental monitoring and to contain harmful outbreaks to public health. We aimed to test an assay for Lp identification among different Legionella species, and to determine the serogroups. Additionally, we investigated the carriage of virulence and antimicrobial resistance genes.MethodsA total of 90 Legionella spp. isolates identified by phenotypic tests were subjected to the designed quantitative PCR assay targeting specific mip for Lp, wzm for Lp1, pvcA and ahpD for biofilm production. Eleven serogroups were investigated in all our isolates tested positive for mip gene, subsequently analyzed for 12 virulence and 8 antimicrobial resistance genes.ResultsOnly the 70 Lp isolates were positive for mip. Out of 27 Lp isolates belonging to serogroup 1 based on agglutination test, 23 (85.2%) carried wzm. The presence of ahpD and pvcA was found in 94.3 and 98.6% of Lp isolates, respectively. By multiplex PCR, all 23 wzm-positive strains were confirmed as serogroup 1 that was the most predominant (33%). At least one virulence gene was detected in all Lp isolates. The most frequent gene was ispE (100%), followed by issD (96%), icmK and enhC (93%), cpxA (91%), rtxA2 (74%), lvhB8-B9 (61%), and prpA (54%). The other genes were less diffused in Lp strains (rtxA1, 44%; lvhB3-B4, 47%; pvcB, 27%; lvrE, 24%). Of the macrolide resistance genes, the ereA was found in 84% of Lp strains, while only 14 (20%) harbored the lpeAB among the efflux pump genes.ConclusionThe assays validated in this study enable the simultaneous Lp and Lp1 detection. The differentiation of Lp strains according to their virulence properties could be useful to predict the bacterial ability to survive and to cause the disease.
The prognostic significance of synchronous metastasis in glioblastoma multiforme patients: a propensity score-matched analysis using SEER data
Authors: Shen Hui , Mei Qing , Chai Xubin , Jiang Yuanfeng , Liu Aihua , Liu Jiachun 
Published: October 2024

BackgroundGlioblastoma multiforme (GBM) with synchronous metastasis(SM) is a rare occurrence. We extracted the data of GBM patients from the SEER database to look into the incidence of SM in GBM, determine the prognostic significance of SM in GBM, and assess therapeutic options for patients presenting with SM.MethodsFrom 2004 to 2015, information on GBM patients was obtained from the Surveillance, Epidemiology, and End Results (SEER) database. The propensity score matching (PSM) method was employed to mitigate confounding factors between SM and non-SM groups, subsequently investigating the prognostic significance of SM in patients with GBM. Multivariate Cox proportional hazards regression analyses were employed to identify independent prognostic variables for GBM patients with SM. A forest plot was used to visualize the results.ResultsA cohort of 19,708 patients was obtained from the database, among which 272 (1.4%) had SM at the time of diagnosis. Following PSM at a 3:1 ratio, in both univariate and multivariate cox regression analysis, SM (HR = 1.27, 95% CI: 1.09–1.46) was found to be an independent predictive predictor for GBM patients. Furthermore, the Cox proportional hazard forest plot demonstrated that independent risk variables for GBM patients with SM included age (Old vs. Young, HR = 1.44, 95% CI: 1.11–1.88), surgery (biopsy vs. no surgery, HR = 0.67, 95% CI: 0.46–0.96;Subtotal resection vs. no surgery, HR = 0.47, 95% CI: 0.32–0.68;Gross total resection vs. no surgery, HR = 0.44, 95% CI: 0.31–0.62), radiotherapy (HR = 0.58, 95% CI: 0.41–0.83), and chemotherapy (HR = 0.51, 95% CI: 0.36–0.72).ConclusionThe predictive value of SM in GBM was determined by this propensity-matched analysis using data from the SEER database. Radiotherapy, chemotherapy, and surgery constitute an effective treatment regimen for patients with SM. A more positive approach toward the use of aggressive treatment for GBM patients with SM may be warranted.
Predictive value of the dynamic systemic immune-inflammation index in the prognosis of patients with intracerebral hemorrhage: a 10-year retrospective analysis
Authors: Ma Wenchao , Wu Xiaoming , Yang Lang , Yang Yumin , Zhang Hao , Wang Yan , Xue Haiying , Long Xiaodong 
Published: October 2024

Background and purposeThe systemic immune-inflammation index (SII) is a novel immune inflammatory marker which has been proven to have excellent predictive value for many diseases. The aim of this study was to investigate the predictive value of SII at different time points after admission for functional outcome at discharge in patients with intracerebral hemorrhage (ICH).MethodsThe clinical data of patients with ICH who were treated at a medical center for neurological diseases in China between October 2012 and April 2022 were analyzed in this retrospective study. The SII was calculated based on neutrophil×platelet/lymphocyte counts and collected within the first 14 days after admission to analyze the dynamic changes. Adverse outcome was defined as a modified Rankin Scale (mRS) score of 4–6 at discharge. The correlation between the SII and the outcome was assessed using univariate and multivariate logistic regression analyses. The ability of SII to predict outcome was evaluated by the area under the receiver operating characteristic (ROC) curve (AUC).ResultsA total of 1,569 patients with ICH were included, of whom 790 had adverse outcome (50.35%). The Univariate logistic regression analysis showed that SII at any time point within 14 days after admission was significantly associated with adverse outcome. In the multivariate logistic regression analysis, the SII within 7 days after admission was found to be an independent predictor of adverse functional outcome in ICH patients at discharge. The ROC curve demonstrated that compared to other time points, the SII on day 2 after admission exhibited stronger predictive power for the functional outcome of patients with ICH at discharge (AUC:0.733, 95%CI = 0.679–0.787) (sensitivity 47.09%, specificity 87.02%) (OR 1.074, 95%CI = 1.033–1.126, p = 0.001).ConclusionSII within 7 days after admission, especially on day 2, is independently associated with adverse functional outcome in ICH patients at discharge. This association could be utilized in clinical practice and warrants further investigation.
Abnormal changes in neuropsychological function, brain structure and cerebral perfusion in patients with unruptured intracranial aneurysms
Authors: Li Wei , Zhang Si , Fan Weijie , Fu Xiaomei , Zhang Dong , Wen Li 
Published: October 2024

BackgroundPatients with unruptured intracranial aneurysms (UIAs) often experience emotional changes and cognitive impairments. However, the specific mechanisms underlying these impairments are still not fully understood.MethodsIn the present study, voxel-based morphometry (VBM) and surface-based morphometry (SBM) were employed to investigate structural alterations in 49 patients diagnosed with UIAs compared with 50 healthy controls. Additionally, this study aimed to analyze the correlations among cortical morphological indices, cerebral blood perfusion values and neuropsychological test results.ResultsCompared with control group, UIA patients exhibited increased gray matter volume in the right anterior orbitofrontal cortex and decreased gray matter volume in the left thalamus pulvinar and hippocampus. Furthermore, the fractal dimension was lower in the right postcentral gyrus and entorhinal cortex. The cerebral perfusion values in the abnormal brain regions demonstrated a downward trend, which was associated with a reduction in gray matter volume in the left thalamus pulvinar and hippocampus, elevated anxiety levels and impaired executive function.ConclusionUIA patients are prone to cognitive impairment and emotional dysregulation, which are accompanied by subtle changes in local gray matter volume and decreases in fractal dimension and cerebral blood flow. These findings provide new insights into the potential mechanisms underlying the cognitive impairment observed in UIA patients.
Effects of health at every size based interventions on health-related outcomes and body mass, in a short and a long term
Authors: Suárez Rosario , Cucalon Gabriela , Herrera Carolina , Montalvan Martha , Quiroz Jestin , Moreno Melissa , Sarmiento-Andrade Yoredy , Cabañas-Alite Luis 
Published: October 2024

ObjectiveThis study aims to provide rapid and up-to-date evidence on the effectiveness of Health at Every Size (HAES) interventions compared to controls or other conventional approaches in individuals with overweight or obesity, with the goal of developing more effective and body-diverse respectful strategies.MethodsA review of literature was carried out using the following databases: PubMed, Scopus, Embase, Web of Science, and SciELO. Research articles were selected based on predefined inclusion and exclusion criteria. Extracted data included study characteristics (design, setting, population demographics, sample size, intervention characteristics, study duration, and follow-up period) and health-related outcomes.ResultsThe search yielded 324 articles, of which 20 articles met the inclusion and exclusion criteria. The majority of studies focused on lifestyle improvement, particularly in nutrition, body image, and relationships with food, utilizing a HAES approach. Additionally, other studies examined outcomes such as general well-being, body weight, body composition, cardiovascular risk, and changes in physical activity. Long-term results were particularly noted in studies incorporating physical activity interventions.ConclusionHAES interventions appear to be a feasible strategy for promoting overall health and wellness, regardless of body size or shape. However, further evaluation is needed to assess the sustainability of these changes and their long-term impact, as current evidence suggest a they may not be maintained over time.