bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repeatability of quantitative sensory testing in healthy cats in a clinical setting with comparison to cats with osteoarthritis
Authors: Addison Elena S , Clements Dylan N 
Published: February 2017

Objectives The aim of this study was to evaluate the repeatability of quantitative sensory tests (QSTs) in a group of healthy untrained cats (n = 14) and to compare the results with those from cats with osteoarthritis (n = 7). Methods Peak vertical force (PVF) and vertical impulse were measured on a pressure plate system. Thermal sensitivity was assessed using a temperature-controlled plate at 7°C and 40°C. Individual paw lifts and overall duration of paw lifts were counted and measured for each limb. Paw withdrawal thresholds were measured using manual and electronic von Frey monofilaments (MVF and EVF, respectively) applied to the metacarpal or metatarsal pads. All measurements were repeated twice to assess repeatability of the tests. Results In healthy cats all tests were moderately repeatable. When compared with cats with osteoarthritis the PVF was significantly higher in healthy hindlimbs in repeat 1 but not in repeat 2. Cats with osteoarthritis of the forelimbs showed a decrease in the frequency of paw lifts on the 7°C plate compared with cats with healthy forelimbs, and the duration of paw lifts was significantly less than healthy forelimbs in the first repeat but not in the second repeat. Osteoarthritic limbs had significantly lower paw withdrawal thresholds with both MVF and EVF than healthy limbs. Conclusions and relevance QSTs are moderately repeatable in untrained cats. Kinetic gait analysis did not permit differentiation between healthy limbs and those with osteoarthritis, but thermal sensitivity testing (cold) does. Sensory threshold testing can differentiate osteoarthritic and healthy limbs, and may be useful in the diagnosis and monitoring of this condition in cats in the clinical setting.
Consumer perspectives on mHealth for weight loss: a review of qualitative studies
Authors: Lyzwinski Lynnette N , Caffery Liam J , Bambling Matthew , Edirippulige Sisira 
Published: February 2017

Introduction With increasing development and use of mobile health (mHealth) interventions for weight loss in overweight and obese populations, it is timely to gain greater insight into consumer experience with these technologies. The aims of this review were to identify common themes across studies that included user preferences for mHealth intervention for weight loss. Methods The databases PubMed (Medline), CINAHL, Web of Science, and Embase were searched for relevant qualitative studies on mHealth for weight loss. Searches were conducted in May 2016. Results Several common high preference themes were identified relating to simple and attractive apps that allowed for self-monitoring with feedback. The five key themes concerning text messages for weight loss involved a careful consideration of personalization, message tone, structure, frequency and content. Key optimization themes for weight loss apps were personalization, simplicity with appeal and engagement/entertainment. Common identified benefits of mHealth for weight loss included self-monitoring, goal setting, feedback, ability to motivate, educate, and remind. Common barriers users identified were related to technological and psychological issues as well as message overload/inappropriate timing of messages. Conclusion When planning an mHealth weight loss intervention, critical factors are the message tone, structure and the frequency of message delivery. Personalization also seems to be important. Designing simple apps while still ensuring that they engage the user is also essential. Additionally, it seems important to tailor the content in accordance with different target group demographic preferences. The successful reach and adoption of mHealth interventions requires minimizing perceived barriers and maximizing perceived benefits.
Helping Survivors of Human Trafficking: A Systematic Review of Exit and Postexit Interventions
Authors: Dell Nathaniel A. , Maynard Brandy R. , Born Kara R. , Wagner Elizabeth , Atkins Bonnie , House Whitney 
Published: February 2017

Background: Human trafficking is a global problem and results in deleterious psychological, social, and physical effects on the lives of those who are trafficked; however, it is not clear how to best intervene with survivors. The purpose of this review was to synthesize the evidence of exit and postexit intervention programs for survivors of human trafficking to inform practice and research. Method: Systematic review methods were used to search, select, and extract data from published and unpublished experimental, quasi-experimental, and preexperimental studies that assessed the effects of any exit or postexit interventions for victims of human trafficking. The authors searched eight databases, reviewed bibliographies, and conducted forward citation searches from relevant reports and prior reviews to find studies authored between 2005 and 2015. Results: The search yielded six eligible studies that included 155 female and 6 male survivors from four countries. Interventions were diverse, with three using a trauma-informed approach. Authors measured a myriad of outcomes, including mental health, social network, community reintegration, and employment; however, the quality of most studies was poor. Discussion: Evidence of effects of exit and postexit interventions is sparse, and much of the research is poorly designed and executed; however, the needs of trafficking survivors are complex and effective interventions are desperately needed. Implications for practice and research are discussed.
Challenges of academic healthcare leaders in a higher education context in South Africa
Authors: Jooste Karien , Frantz Jose , Waggie Firdouza 
Published: February 2017

Universities are in a highly competitive environment, needing strong academic leadership. Some heads of departments have been appointed into leadership positions in a healthcare faculty after having been mere academics for a few years. They are more likely to experience challenges. This study aimed to explore the views and understanding of heads of departments in a healthcare faculty on being appointed as academic leaders in a higher education context in South Africa. A qualitative design using 12 individual unstructured interviews was conducted with all the heads of departments in a health sciences faculty. Open coding was conducted and two themes emerged, focusing on the varied skills needed for academic leadership positions and developing leadership skills amongst senior academics. The findings indicated that development of senior academics in leadership should be undertaken by a knowledgeable professional in formal or informal settings, to encourage mentorship and more regular group meetings, while addressing the core role of a leader. Implications of these findings for a faculty of health sciences and suggestions for leadership succession in future are discussed.
Risk of Nonfatal Stroke in Type 2 Diabetes Mellitus Patients: A Retrospective Comparison Between Disease Management Programs and Standard Care
Authors: Wiefarn Stefan , Heumann Christian , Rettelbach Anja , Kostev Karel 
Published: February 2017

Objective: The present retrospective study examines the influence of disease management programs on nonfatal stroke in type 2 diabetes mellitus (T2DM) patients in Germany. Methods: The evaluation is based on retrospective patient data from the Disease Analyzer (IMS Health). The analysis included 169 414 T2DM patients aged 40 years and older with an initial prescription of antihyperglycemic therapy between January 2004 and December 2014. A total of 86 713 patients participated in a disease management program (DMP) for T2DM and 82 701 patients received standard care. The main outcome measure of this study was nonfatal stroke. Kaplan-Meier curves of DMP and SC patients were compared using log rank test. The Cox proportional hazards model was used to provide an adjusted estimate of the DMP effect. Results: It is apparent from the baseline characteristics that the general health of patients receiving standard care was poorer than that of patients participating in a DMP. The baseline HbA1c value was 7.6% in the DMP group and 7.8% in the SC group. Furthermore, the SC group had a higher proportion of preexisting conditions, such as coronary heart disease (CHD), peripheral arterial occlusive disease (pAOD), and renal insufficiency. The proportion of patients who received insulin in first year therapy was higher in the SC group. Time to event analysis showed that DMP was associated with a delayed occurrence of stroke, because stroke occurred an average of 350 days later in DMP patients than in patients receiving SC (DMP: 1.216 days, RV: 866 days). The Cox model with covariable adjustment confirmed the significant association of DMPs with nonfatal stroke in patients with type 2 diabetes mellitus (HR 0.71; 95% CI: 0.69-0.74). Conclusion: The present study indicates that DMPs are positively associated with stroke. The possible reasons for this must be verified in further studies.
Prophylaxis usage, bleeding rates, and joint outcomes of hemophilia, 1999 to 2010: a surveillance project
Authors: Manco-Johnson Marilyn J. , Soucie J. Michael , Gill Joan Cox 
Published: February 2017

Key Points
Bleeding in US hemophilia patients has decreased dramatically in parallel with increased use of prophylaxis. Only prophylaxis started before age 4 years preserves normal joint function.
Expression and regulation of C/EBPα in normal myelopoiesis and in malignant transformation
Authors: Avellino Roberto , Delwel Ruud 
Published: February 2017

AbstractOne of the most studied transcription factors in hematopoiesis is the leucine zipper CCAAT-enhancer binding protein α (C/EBPα), which is mainly involved in cell fate decisions for myeloid differentiation. Its involvement in acute myeloid leukemia (AML) is diverse, with patients frequently exhibiting mutations, deregulation of gene expression, or alterations in the function of C/EBPα. In this review, we emphasize the importance of C/EBPα for neutrophil maturation, its role in myeloid priming of hematopoietic stem and progenitor cells, and its indispensable requirement for AML development. We discuss that mutations in the open reading frame of CEBPA lead to an altered C/EBPα function, affecting the expression of downstream genes and consequently deregulating myelopoiesis. The emerging transcriptional mechanisms of CEBPA are discussed based on recent studies. Novel insights on how these mechanisms may be deregulated by oncoproteins or mutations/variants in CEBPA enhancers are suggested in principal to reveal novel mechanisms of how CEBPA is deregulated at the transcriptional level.
The GATA factor revolution in hematology
Authors: Katsumura Koichi R. , Bresnick Emery H. ,   the GATA Factor Mechanisms Group
Published: February 2017

AbstractThe discovery of the GATA binding protein (GATA factor) transcription factor family revolutionized hematology. Studies of GATA proteins have yielded vital contributions to our understanding of how hematopoietic stem and progenitor cells develop from precursors, how progenitors generate red blood cells, how hemoglobin synthesis is regulated, and the molecular underpinnings of nonmalignant and malignant hematologic disorders. This thrilling journey began with mechanistic studies on a β-globin enhancer- and promoter-binding factor, GATA-1, the founding member of the GATA family. This work ushered in the cloning of related proteins, GATA-2-6, with distinct and/or overlapping expression patterns. Herein, we discuss how the hematopoietic GATA factors (GATA-1-3) function via a battery of mechanistic permutations, which can be GATA factor subtype, cell type, and locus specific. Understanding this intriguing protein family requires consideration of how the mechanistic permutations are amalgamated into circuits to orchestrate processes of interest to the hematologist and more broadly.
GATA factor mutations in hematologic disease
Authors: Crispino John D. , Horwitz Marshall S. 
Published: February 2017

Abstract
GATA family proteins play essential roles in development of many cell types, including hematopoietic, cardiac, and endodermal lineages. The first three factors, GATAs 1, 2, and 3, are essential for normal hematopoiesis, and their mutations are responsible for a variety of blood disorders. Acquired and inherited GATA1 mutations contribute to Diamond-Blackfan anemia, acute megakaryoblastic leukemia, transient myeloproliferative disorder, and a group of related congenital dyserythropoietic anemias with thrombocytopenia. Conversely, germ line mutations in GATA2 are associated with GATA2 deficiency syndrome, whereas acquired mutations are seen in myelodysplastic syndrome, acute myeloid leukemia, and in blast crisis transformation of chronic myeloid leukemia. The fact that mutations in these genes are commonly seen in blood disorders underscores their critical roles and highlights the need to develop targeted therapies for transcription factors. This review focuses on hematopoietic disorders that are associated with mutations in two prominent GATA family members, GATA1 and GATA2.
Protean proteases: at the cutting edge of lung diseases
Authors: Taggart Clifford , Mall Marcus A. , Lalmanach Gilles , Cataldo Didier , Ludwig Andreas , Janciauskiene Sabina , Heath Nicole , Meiners Silke , Overall Christopher M. , Schultz Carsten , Turk Boris , Borensztajn Keren S. 
Published: February 2017

Proteases were traditionally viewed as mere protein-degrading enzymes with a very restricted spectrum of substrates. A major expansion in protease research has uncovered a variety of novel substrates, and it is now evident that proteases are critical pleiotropic actors orchestrating pathophysiological processes. Recent findings evidenced that the net proteolytic activity also relies upon interconnections between different protease and protease inhibitor families in the protease web.In this review, we provide an overview of these novel concepts with a particular focus on pulmonary pathophysiology. We describe the emerging roles of several protease families including cysteine and serine proteases.The complexity of the protease web is exemplified in the light of multidimensional regulation of serine protease activity by matrix metalloproteases through cognate serine protease inhibitor processing. Finally, we will highlight how deregulated protease activity during pulmonary pathogenesis may be exploited for diagnosis/prognosis purposes, and utilised as a therapeutic tool using nanotechnologies.Considering proteases as part of an integrative biology perspective may pave the way for the development of new therapeutic targets to treat pulmonary diseases related to intrinsic protease deregulation.
Tussive challenge with ATP and AMP: does it reveal cough hypersensitivity?
Authors: Fowles Helen Elizabeth , Rowland Tim , Wright Caroline , Morice Alyn 
Published: February 2017

Recent studies have demonstrated that blockade of P2X3 ATP receptors can profoundly inhibit chronic cough. We have considered whether inhaled ATP produces a tussive response and whether chronic cough patients are hypersensitive to inhaled ATP compared to healthy volunteers.A standardised inhalational cough challenge was performed with ATP and AMP. We randomised 20 healthy volunteers and 20 chronic cough patients as to the order of challenges. The concentration of challenge solution causing at least five coughs (C5) was compared for ATP and AMP.The study population consisted of six male and 14 female volunteers in each group. Two out of 19 healthy volunteers coughed with AMP (one volunteer could not take part in this challenge) and none reached C5. Eight out of 20 chronic cough patients coughed with AMP and two reached C5. Of the 20 healthy volunteers, 18 coughed with ATP, with 15 reaching C5. All 19 chronic cough patients completing the ATP challenge coughed with ATP and 18 reached C5. The chronic cough patients had a greater cough response at lower concentrations of ATP.The greater potency of ATPversusAMP in the inhalational challenge suggests that tussive responses are mediated through members of the P2X purinergic receptor family. This acute effect was, however, not sufficient to explain cough hypersensitivity syndrome.
Smoking reduces circulating CD26hiCD161hi MAIT cells in healthy individuals and patients with multiple sclerosis
Authors: Ammitzbøll Cecilie , Börnsen Lars , Romme Christensen Jeppe , Ratzer Rikke , Romme Nielsen Birgitte , Søndergaard Helle B , von Essen Marina R , Sellebjerg Finn 
Published: February 2017

Abstract
Upon chronic cigarette smoke exposure, inhaled antigens and irritants cause altered lung immune homeostasis. Circulating immune cells are affected, and smoking is associated with an increased risk of developing various disorders, including multiple sclerosis (MS). This study was conducted to determine the impact of smoking on circulating immune cell subsets. Furthermore, we determined whether any smoking-associated changes were related to MS. With the use of flow cytometry, CFSE assays, and ELISpot assays, we analyzed circulating immune cell phenotypes and quantified antigen-induced proliferation and cytokine secretion in smokers and nonsmokers in a cohort of 100 healthy individuals (HI). In addition, we analyzed immune cell subsets associated with smoking in 2 independent cohorts of patients with MS. In HI smokers compared with nonsmokers, we found increased blood cell counts of granulocytes, monocytes, and lymphocytes. These cells were not more proinflammatory, autoreactive, or EBV reactive compared with cells from nonsmokers. Phenotypic differences were seen in plasmacytoid dendritic cells (pDCs) and CD8+ T cells as higher percentages of ICOS ligand (ICOSL)+ pDCs and lower percentages of CD26hiCD161hi CD8+ T cells and CCR6+ CD8+ T cells in smokers compared with nonsmokers. In supplemental analyses, we showed that CD26hiCD161hi CD8+ T cells were mainly mucosal-associated invariant T cells (MAITs). Comparable frequencies of ICOSL+ pDCs, CCR6+ CD8+ T cells, and CD26hiCD161hi CD8+ T cells were found between HI and MS patients who were nonsmokers. Our findings suggest general proinflammatory effects from smoking combined with skewing of specific cell populations in HI and MS patients. The function of these cell populations needs further investigation.
First report of the East African kdr mutation in an Anopheles gambiae mosquito in Côte d’Ivoire
Authors: Chouaïbou Mouhamadou , Kouadio Fodjo Behi , Tia Emmanuel , Djogbenou Luc 
Published: February 2017

Background. The intensive use of insecticides in public health and agriculture has led to the development of insecticide resistances in malaria vectors across sub-Saharan Africa countries in the last two decades. The kdr target site point mutation which is among the best characterised resistance mechanisms seems to be changing its distribution patterns on the African continent. The 1014F kdr mutation originally described only in West Africa is spreading to East Africa while the 1014S kdr mutation originally described in East Africa, is spreading to West and Central Africa. However, the East-kdr mutation has not been reported in Côte d'Ivoire so far. Methods. Immature stages of Anopheles gambiae s.l. were collected from breeding sites at the outskirts of Yamoussoukro, Côte d'Ivoire. Emerging 3–5 day old adult female mosquitoes were tested for susceptibility to deltamethrin 0.05%, malathion 5%, bendiocarb 1% and dichlorodiphenyltrichloroethane (DDT) 4% according to WHO standard procedures. A total of 50 An. gambiae s.l. specimens were drawn at random for DNA extraction and identification down to the species level. A subsample of 30 mosquitoes was tested for the East-African kdr mutation using a Taqman assay. Results. The tested mosquito population appeared to be strongly resistant to deltamethrin (1.03% mortality), bendiocarb (38.46% mortality) and DDT (0% mortality) with probable resistance observed for malathion (92.47%). Among the 41 mosquitoes that were successfully characterized, An. coluzzii was predominant (68.3%) followed by An. gambiae s.s. (19.5%) and a few hybrids (7.3%). Out of 30 specimens genotyped for East-kdr, a single hybrid mosquito appeared to be heterozygous for the mutation. Conclusion. The present study revealed the presence of the East-kdr mutation in Côte d’Ivoire for the first time in An. gambiae and highlights the urgent need to start monitoring the allele and genotype frequencies.
Clinical evaluation of a bioactive beta-glucan gel in the treatment of ‘hard-to-heal’ wounds
Authors: King B. , Barrett S. , Cutting K.F. 
Published: February 2017

Objective: The aim of this evaluation is to assess the effects of a wound healing gel in a wounds of different aetiologies. Method: Data was recorded on the wound surface area, tissue type, and patient level of wound pain at baseline (0) and at weeks 1, 2, 3, 4, and 8. Results: Of the total 39 patients enrolled in the study, 26 patients who complied with the protocol criteria completed the minimum four-week study period. During the 12-week evaluation period, seven of the 26 wounds fully healed and an additional eight wounds showed a reduction in size of more than 50 %. Of the remaining 11, five wounds showed moderate healing progression and six wounds did not respond to treatment. Following the 12 week evaluation time point clinicians reported that a further three wounds healed—a 38 % healing rate. Conclusion: The results give promise that this advanced gel, containing a macrophage activating substance, can be a tool in re-activating healing in stalled wounds where standard of care is no longer giving the desired healing progression.
The effects ofTarantula cubensisvenom on open wound healing in rats
Authors: Gul Satar N.Y. , Cangul I.T. , Topal A. , Kurt H. , Ipek V. , Onel G.I. 
Published: February 2017

Objective:The aim of this study was to evaluate the clinical and histopathological effects of two different dosages of alcohol extract of Tarantula cubensis (Theranekron) on open wounds.Method:A total of 24 Sprague-Dawley rats were randomly divided into Tarantula cubensis extract (TCE1, n=8) 1/10 diluted, TCE (TCE2, n=8), and (3) vehicle-control (0.2 ml of 96 % ethanol, n=8) groups. Experimental full-thickness 1 x 1cm wounds were created on dorsum skin. TCE or vehicle were given systemically by subcutaneous injections on postoperative days 1 and 4. Wound planimetry and procurement of biopsies was performed on days 4, 8, 12 and 16.Results:The mean non-epithelialised wound area in the vehicle-control group was significantly larger than in the TCE1 group on days 4, 8, 12 and 16, and in the TCE2 group on days 8, 12 and 16 (p<0.05). The mean percentage of wound contraction was significantly higher in the TCE1 and TCE2 groups than in the vehicle control group on days 8, 12 and 16 (p<0.05). Histopathologically, wound healing was characterised by a significant decrease in the neutrophil counts and a significant increase in neovascularisation; neither were effected by TCE.Conclusion:Our results suggest that alcohol extract of Tarantula cubensis accelerates epithelialisation and, thus, has beneficial effects on open wound healing in rats.