bluuebunny/crossref_metadata_2025_split huggingface dataset:
NRSF–mediated repression of neuronal genes in developing brain persists in the absence of NRSF-Sin3 interaction
Authors: Hall Alicia M. , Short Annabel K. , Singh-Taylor Akanksha , Daglian Jennifer , Mishina Tadashi , Schmidt William K. , Kouji Hiroyuki , Baram Tallie Z. 
Published: January 2018

AbstractRepression of target genes by the transcriptional repressor neuronal restrictive silencing factor (NRSF)/repressor element 1 silencing transcription factor (REST) contributes to enduring plasticity in the developing brain. However, the cofactor(s) interacting with NRSF to enable target gene repressor are not well understood, and may vary among neuronal populations and brain regions as well as with different contexts. Here we employed the novel designer drug mS-11 to block the interactions of the cofactor Sin3 with NRSF. We tested if NRSF-Sin3 interaction is required for repression of NRSF target genes in developing hypothalamus after activity-dependent modulation of NRSF function. In the hypothalamus in vitro, blocking glutamatergic neurotransmission robustly increased NRSF binding to the target gene Crh, resulting in its repression. Blocking the binding of NRSF to the chromatin with decoy NRSE-oligodeoxynucleotides abrogated this repression. In contrast, mS-11 at several concentrations did not impede Crh repression. NRSF-mediated repression may underlie disease processes such as the onset of epilepsy. Therefore, identifying small-molecule antagonists of NRSF is crucial for the development of disease-preventing or modifying interventions.
Enzyme-based synthesis of single molecule RNA FISH probes
Authors: Lanctôt Christian 
Published: January 2018

ABSTRACTSingle molecule RNA fluorescence in situ hybridization (smRNA FISH) allows the quantitative analysis of gene expression in single cells. The technique relies on the use of pools of end-labeled fluorescent oligonucleotides to detect specific cellular RNA sequences. These fluorescent probes are currently chemically synthesized. Here I describe a novel technique based on the use of routine molecular biology enzymes to generate smRNA FISH probes without the need for chemical synthesis of pools of oligonucleotides. The protocol comprises 3 main steps: purification of phagemid-derived single stranded DNA molecules comprising a segment complementary to a target RNA sequence; fragmentation of these molecules by limited DNase I digestion; and end-labeling of the resulting oligonucleotides with terminal deoxynucleotide transferase and fluorescent dideoxynucleotides. smRNA FISH probes that are obtained using the technique presented here are shown to perform as well as conventional probes. The main advantages of the method are the low cost of probes and the flexibility it affords in the choice of labels. Enzyme-based synthesis of probes should further increase the popularity of smRNA FISH as a tool to investigate gene expression at the cellular or subcellular level.
The Effect of Dissociation between Proprioception and Vision on Perception and Grip Force Control in a Stiffness Judgment Task
Authors: Hu Stephanie , Leib Raz , Nisky Ilana 
Published: January 2018

AbstractOur sensorimotor system estimates stiffness to form stiffness perception, such as for choosing a ripe fruit, and to generate actions, such as to adjust grip force to avoid slippage of a scalpel during surgery. We examined how temporal manipulation of the haptic and visual feedback affect stiffness perception and grip force adjustment during a stiffness discrimination task. We used delayed force feedback and delayed visual feedback to break the natural relations between these modalities when participants tried to choose the harder spring between pairs of springs. We found that visual delay caused participants to slightly overestimate stiffness while force feedback delay caused a mixed effect on perception; for some it caused underestimation and for some overestimation of stiffness. Interestingly and in contrast to previous findings without vision, we found that participants increased the magnitude of their applied grip force for all conditions. We propose a model that suggests that this increase was a result of coupling the grip force adjustment to their proprioceptive hand position, which was the only modality which we could not delay. Our findings shed light on how the sensorimotor system combines information from different sensory modalities for perception and action. These results are important for the design of improved teleoperation systems that suffer from unavoidable delays.
Measurement of the Persistence Length of Cytoskeletal Filaments using Curvature Distributions
Authors: Wisanpitayakorn Pattipong , Mickolajczyk Keith J. , Hancock William O. , Vidali Luis , Tüzel Erkan 
Published: January 2018

AbstractCytoskeletal filaments such as microtubules and actin filaments play important roles in the mechanical integrity of cells and the ability of cells to respond to their environment. Measuring the mechanical properties of cytoskeletal structures is crucial for gaining insight into intracellular mechanical stresses and their role in regulating cellular processes. One of the ways to characterize these mechanical properties is by measuring their persistence length, the average length over which filaments stay straight. There are several approaches in the literature for measuring filament deformations, including Fourier analysis of images obtained using fluorescence microscopy. Here, we show how curvature distributions can be used as an alternative tool to quantify bio-filament deformations, and investigate how the apparent stiffness of filaments depends on the resolution and noise of the imaging system. We present analytical calculations of the scaling curvature distributions as a function of filament discretization, and test our predictions by comparing Monte Carlo simulations to results from existing techniques. We also apply our approach to microtubules and actin filaments obtained fromin vitrogliding assay experiments with high densities of non-functional motors, and calculate the persistence length of these filaments. The presented curvature analysis is significantly more accurate compared to existing approaches for small data sets, and can be readily applied to bothin vitroorin vivofilament data through the use of an ImageJ plugin we provide.
Mind the mindset! The interaction of proactive personality, transformational leadership and growth mindset for engagement at work
Authors: Caniëls Marjolein C.J. , Semeijn Judith H. , Renders Irma H.M. 
Published: January 2018

PurposeThe purpose of this paper is to analyze whether and how employees’ proactive personality is related to work engagement. Drawing on job demands-resources theory, the study proposes that this relationship is moderated by a three-way interaction between proactive personality × transformational leadership × growth mindset.Design/methodology/approachThe study is based on survey data from 259 employees of an internationally operating high-tech organization in the Netherlands.FindingsIn line with prior studies, support is found for positive significant relationships of proactive personality and transformational leadership with engagement. Additionally, transformational leadership is found to moderate the relationship between proactive personality and work engagement, but only when employees have a growth mindset.Originality/valueThe study advances the literature that investigates the proactive personality-engagement relationship. Specifically, this study is the first to examine a possible three-way interaction that may deepen the insights for how proactive personality, transformational leadership and growth mindset interact in their contribution to work engagement.
When do regulations matter for bank risk-taking? An analysis of the interaction between external regulation and board characteristics
Authors: De Vita Glauco , Luo Yun 
Published: January 2018

PurposeAccording to previous international studies, the impact of external regulation on bank risk is ambiguous. The purpose of this paper is to ask the question, “When do regulations matter for bank risk-taking?” by reporting the first empirical investigation of how the relation between bank regulations (capital requirements, official supervisory power and market discipline) and bank risk-taking is moderated by board monitoring characteristics.Design/methodology/approachUsing SYS-GMM, the analysis of the interaction between bank-level boards of directors’ attributes (board size, board independence and board gender diversity) and external regulation is based on a sample of 493 banks operating in 54 countries over 2001-2015, accounting for three measures of bank risk-taking.FindingsRegulations matter for bank risk-taking conditional on board characteristics: board size, board independence and board diversity. With the exception of capital requirements, the market discipline exerted by external private monitoring and greater supervisory power are unable to mitigate the propensity to greater risk-taking by banks resulting from larger board size, higher board independence and greater gender diversity of the board.Originality/valueThe bank risk empirical literature is still silent as to the interaction between board governance and regulation for the purpose of examining banks’ risk-taking. This paper fills this gap, thus making a significant contribution by extending our knowledge of whether and how board governance moderates the relationship between external regulation and bank risk-taking.
The contribution of foresight to improve long-term security planning
Authors: Jore Sissel Haugdal , Utland Inger-Lise Førland , Vatnamo Victoria Hell 
Published: January 2018

PurposeDespite the common focus on studying future events, the study of risk management and foresight have developed as two segmented scientific fields. This study aims to investigate whether current risk management methodology is sufficient for long-term planning against threats from terrorism and other black swan events, and whether perspectives from foresight studies can contribute to more effective long-term security planning.Design/methodology/approachThis study investigates the planning process of the rebuilding of the Norwegian Government Complex destroyed during a terrorist attack in 2011. The study examines whether security risk managers find current security risk management methodology sufficient for dealing with long-term security threats to the Norwegian Government Complex.FindingsCurrent security risk management methodology for long-term security planning is insufficient to capture black swan events. Foresight perspectives could contribute by engaging tools to mitigate the risk of these events. This could lead to more robust security planning.Originality/valueThe main contribution of this paper is to investigate whether perspectives and methodology from foresight studies can improve current security risk management methodology for long-term planning and look for cross-fertilization between foresight and risk studies. A framework for scenario development based on security risk management methodology and foresight methodology is proposed that can help bridge the gap.
Knowledge intensive business services: ambiguities and continuities
Authors: Miles Ian Douglas , Belousova Veronika , Chichkanov Nikolay 
Published: January 2018

Purpose
The substantial growth in literature on knowledge-intensive business services (KIBSs) has thrown light on their contributions to innovation and innovation systems. This paper is the first of a set that examines major debates and conclusions to have emerged from this growing body of evidence.

Design/methodology/approach
This is a review essay, which also presents relevant statistics. It addresses definitional issues and controversies, and sets out basic trends and characteristics of the KIBS industries. The focus is mainly on KIBS firms, though the production of similar services in other types of organisation is also considered.

Findings
Many of the conclusions of an earlier (2005) review in this journal remain valid, though difficulties in capturing these activities in official statistics mean that there are many issues that demand closer inspection. Understanding the role and future prospects of KIBS will also require looking beyond the literature that focuses just on KIBS industries.

Research limitations/implications
This study involves literature review and statistical analysis. Future work would benefit from involvement of practitioners and users of KIBS.

Practical implications
More explicit consideration of KIBS in statistical frameworks is still required, and novel approaches to data conceptualisation and production should be explored.

Originality/value
The growing literature on KIBS, and its implications for understanding the roles and future development of the firms and their relationships to innovation systems, requires systematic analysis. Available statistics have been brought together, and this paper also reflects critically on the trajectories of research on these topics.
Exploring work-family interface for Indian women entrepreneurs
Authors: Khandelwal Preetam , Sehgal Aakanksha 
Published: January 2018

PurposeThe purpose of this paper is to study the nature of work-family interface for urban Indian women entrepreneurs (WEs), in terms of not only conflict but also enrichment. It also endeavours to examine the coping and support mechanisms that are used by the WEs.Design/methodology/approachThis paper has adopted a qualitative design, employing in-depth interviews to gain an insight into the research questions.FindingsThough seen to experience work-family conflict extensively, WEs effectively utilized various flexible work and time management techniques as coping mechanisms. More importantly, they were seen to establish a “buy-in” for their work, thereby eliciting support from diverse sources in the home environment. Furthermore, work-to-family enrichment (WFE) was seen to facilitate a stronger bond with family members as well as increase the subjective well-being of the WE. Finally, family-to-work enrichment (FWE) enabled the WEs to obtain various kinds of resources from the family domain to benefit their businesses, thereby affecting venture-level outcomes positively.Research limitations/implicationsAs the findings of the study map reasonably well to prior theoretical research, the present study serves as a starting point for future research examining the relevance of work-family dynamics for WEs across cultures.Practical implicationsThe results of the study positively contribute to the discussion on work-family interface for WEs in the cultural context of India.Originality/valueThe study offers qualitative insights into the work-family dynamics of Indian WEs, especially focusing on the key advantages that they derive by capitalizing on positive spill-over from one domain to the other.
The six “P” model: principles of coaching for inclusion coaches
Authors: Gallagher Tiffany L. , Bennett Sheila M. 
Published: January 2018

Purpose
The purpose of this paper is to identify a set of principles that are necessary to overcome the challenges that inclusion coaches encounter with teachers as they transition into an inclusive service delivery model.

Design/methodology/approach
Online written reflections of 13 inclusion coaches (K-12) who were a part of a larger, mixed-methods research design are the primary data source. For the two years of the project, the inclusion coaches provided bi-annual reflections, each with 7-11 entries. The reflections were downloaded, coded, collapsed, and thematically presented as the inclusion coaches’ perspectives for supporting teachers’ inclusive classroom practices.

Findings
The findings are presented as six principles for the process of coaching teachers for inclusion: pre-requisite: teachers’ receptivity; process: from building trust to collaborating and reflecting; precipice: tension between knowledge and beliefs; promotion: administrative support; proof: evidence of change, impact, and capacity building; and promise: future of the role.

Practical implications
These six principles of coaching for inclusion offer considerations, conditions, and guides for inclusion coaches that are striving for fully inclusive classrooms in their jurisdictions. With a view to future practice, the six principles are reiterative as they should be revisited each time a coaching interaction is initiated in a school site and with a classroom teacher.

Originality/value
As a conclusion, a conceptual model is offered. This spiraling staircase displays the conditions that exist prior to coaching and during coaching interactions and considerations for coaching sustainability.
The role of the mentor in professional knowledge development across four professions
Authors: Peiser Gillian , Ambrose John , Burke Beverley , Davenport Jackie 
Published: January 2018

Purpose
Against a British policy backdrop, which places an ever- increasing emphasis on workplace learning in pre-service professional programmes, the purpose of this paper is to investigate the contribution of the mentor to professional knowledge development in nursing, paramedicine, social work and teaching.

Design/methodology/approach
Taking the form of a literature review, it explores the influence of policy, professional and theoretical conceptualisations of the mentor role, and structural factors influencing the mentor’s contribution to professional knowledge.

Findings
Where there are clearly delineated policy obligations for the mentor to “teach”, mentors are more likely to make connections between theoretical and practical knowledge. When this responsibility is absent or informal, they are inclined to attend to the development of contextual knowledge with a consequent disconnect between theory and practice. In all four professions, mentors face significant challenges, especially with regard to the conflict between supporting and assessor roles, and the need to attend to heavy contractual workloads, performance targets and mentoring roles in tandem.

Practical implications
The authors argue first for the need for more attention to the pedagogy of mentoring, and second for structural changes to workload allocations, career progression and mentoring education. In order to develop more coherent and interconnected professional knowledge between different domains, and the reconciliation of different perspectives, it would be useful to underpin mentoring pedagogy with Bhabba’s notion of “third space”.

Originality/value
The paper makes a contribution to the field since it considers new obligations incumbent on mentors to assist mentees in reconciling theoretical and practical knowledge by the consequence of policy and also takes a multi-professional perspective.
Monitoring multinomial logistic profiles in Phase I using log-linear models
Authors: Izadbakhsh Hamidreza , Noorossana Rassoul , Niaki Seyed Taghi Akhavan 
Published: January 2018

Purpose
The purpose of this paper is to apply Poisson generalized linear model (PGLM) with log link instead of multinomial logistic regression to monitor multinomial logistic profiles in Phase I. Hence, estimating the coefficients becomes easier and more accurate.

Design/methodology/approach
Simulation technique is used to assess the performance of the proposed algorithm using four different control charts for monitoring.

Findings
The proposed algorithm is faster and more accurate than the previous algorithms. Simulation results also indicate that the likelihood ratio test method is able to detect out-of-control parameters more efficiently.

Originality/value
The PGLM with log link has not been used to monitor multinomial profiles in Phase I.
Personality characteristics and Six Sigma: a review
Authors: Witt Phillip Wilson , Baker Timothy 
Published: January 2018

PurposeFrom two bodies of literature, the purpose of this paper is to generate theory for an updated conceptual model of drivers of Six Sigma project success by integrating extant psychology theory and empirical general team project results with a history of eight recent Six Sigma projects and extant Six Sigma literature. The new theory emphasizes the need for project leads to process information simultaneously, as well as develop prioritization abilities. Also, the new theory reverses the relations of three existing theories from general team composition theory. The new theory suggests that Six Sigma belt trainers should focus more on soft skill development.Design/methodology/approachA literature review of the two bodies of literature previously mentioned.FindingsEight new propositions related to the success of Six Sigma projects are developed. Further, two new constructs, “project leader simultaneity of thought” and “prioritization ability” are suggested for further investigation.Originality/valueThe authors suggest a few practical implications: first a greater emphasis on soft skill training would be beneficial in Six Sigma belt training. A second new point of emphasis in belt training is developing greater internal locus of control in belt candidates. Third, the authors suggest that during the team member selection process a diversity of backgrounds would be beneficial to Six Sigma project success.
Empirical investigation of contributions of 5S practice for realizing improved competitive dimensions
Authors: Randhawa Jugraj Singh , Ahuja Inderpreet Singh 
Published: January 2018

Purpose
The purpose of this paper is to evaluate the capabilities of 5S program to meliorate the different competitive dimensions of manufacturing organizations and extracts the various significant factors which influence its successful implementation.

Design/methodology/approach
The paper involves empirical study of responses collected from various manufacturing organizations to investigate the inter-relationships between various 5S implementation success factors and competitive dimensions parameters through different statistical techniques. The study deploys Bonferroni post hoc test to establish the statistical significance of competitive dimensions improvements, progressively accrued over a reasonable period of time, through holistic 5S implementation.

Findings
The study validates the contributions of 5S program toward realization of significant improvements of various competitive dimensions such as overall organizational achievements, production achievements, quality and continuous improvement achievements, cost optimization achievements, employee-related achievements, effective workplace utilization and safety enhancement achievements accrued by Indian manufacturing organizations. Finally the discriminant validity test has been used in the study for classifying highly successful and moderately successful organizations.

Originality/value
The research aims to empirically test the relationships among various 5S implementation constructs and competitive dimensions parameters constructs in manufacturing organizations. The research work establishes that 5S initiatives have effectively contributed for realization of significant competitive dimensions, progressively from introduction to maturity phases. The research highlights the key significant implications for manufacturing managers.