bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translesion synthesis DNA polymerase η exhibits a specific RNA extension activity and a transcription-associated function
Authors: Gali Vamsi K. , Balint Eva , Serbyn Nataliia , Frittmann Orsolya , Stutz Francoise , Unk Ildiko 
Published: October 2017

AbstractPolymerase eta (Polη) is a low fidelity translesion synthesis DNA polymerase that rescues damage-stalled replication by inserting deoxy-ribonucleotides opposite DNA damage sites resulting in error-free or mutagenic damage bypass. In this study we identify a new specific RNA extension activity of Polη of Saccharomyces cerevisiae. We show that Polη is able to extend RNA primers in the presence of ribonucleotides (rNTPs), and that these reactions are an order of magnitude more efficient than the misinsertion of rNTPs into DNA. Moreover, during RNA extension Polη performs error-free bypass of the 8-oxoguanine and thymine dimer DNA lesions, though with a 103 and 102–fold lower efficiency, respectively, than it synthesizes opposite undamaged nucleotides. Furthermore, in vivo experiments demonstrate that the transcription of several genes is affected by the lack of Polη, and that Polη is enriched over actively transcribed regions. Moreover, inactivation of its polymerase activity causes similar transcription inhibition as the absence of Polη. In summary, these results suggest that the new RNA synthetic activity of Polη can have in vivo relevance.
Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions
Authors: Hanna Hanna , Denzi Agnese , Liberti Micaela , André Franck M. , Mir Lluis M. 
Published: October 2017

AbstractMicrosecond pulsed electric fields (μsPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this technology is complex to use. Here we report that the endoplasmic reticulum (ER) membrane can also be electropermeabilized by one 100 µs pulse without affecting the cell viability. Indeed, using Ca2+ as a permeabilization marker, we observed cytosolic Ca2+ peaks in two different cell types after one 100 µs pulse in a medium without Ca2+. Thapsigargin abolished these Ca2+ peaks demonstrating that the calcium is released from the ER. Moreover, IP3R and RyR inhibitors did not modify these peaks showing that they are due to the electropermeabilization of the ER membrane and not to ER Ca2+ channels activation. Finally, the comparison of the two cell types suggests that the PM and the ER permeabilization thresholds are affected by the sizes of the cell and the ER. In conclusion, this study demonstrates that µsPEF, which are easier to control than nsPEF, can permeabilize internal membranes. Besides, μsPEF interaction with either the PM or ER, can be an efficient tool to modulate the cytosolic calcium concentration and study Ca2+ roles in cell physiology.
Distraction and Expressive Suppression Strategies in Regulation of High- and Low-Intensity Negative Emotions
Authors: Li Ping , Wang Wei , Fan Cong , Zhu Chuanlin , Li Shuaixia , Zhang Zhao , Qi Zhengyang , Luo Wenbo 
Published: October 2017

AbstractThe current study compared the effectiveness of distraction, an antecedent-focused strategy that involves diverting an individual’s attention away from affective terms, and expressive suppression, a response-focused strategy that involves inhibiting conscious emotion-expressive behavior during an emotionally aroused state, in the regulation of high- and low-intensity unpleasant stimuli, using event-related potentials (ERPs). Sixteen participants completed an emotion regulation experiment in which they passively viewed high- or low-intensity unpleasant images (view), solved a mathematical equation presented on high- or low-intensity negative images (distraction), or suppressed their emotional expression in response to high- or low-intensity unpleasant images (suppression). Their negative experiences after implementation of these strategies were rated by participants on a 1–9 scale. We mainly found that compared with expressive suppression, distraction yielded greater attenuation of the early phase of centro-parietal LPP when the participants responded to high-intensity stimuli. In the low-intensity condition, distraction, but not expressive suppression, effectively decreased the early phase of LPP. The findings suggest that expressive suppression works as early as distraction in the high-intensity condition; more importantly, distraction is superior to expressive suppression in regulating negative emotion, which is influenced by emotional intensity.
Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression
Authors: Glasner Ariella , Isaacson Batya , Viukov Sergey , Neuman Tzahi , Friedman Nehemya , Mandelboim Michal , Sexl Veronika , Hanna Jacob H. , Mandelboim Ofer 
Published: October 2017

AbstractNatural Killer (NK) cells employ activating receptors like the Natural Cytotoxicity Receptors (NCRs: NKp30, NKp44 and NKp46), of which only NKp46 has a mouse orthologue (Ncr1), to eliminate abnormal cells. NKp46/Ncr1 is considered a selective marker for NK cells, although it is also found on a subset of ILCs, where it appears to be without function. The influenza virus hemagglutinin (HA) was the first ligand identified for Ncr1/NKp46 followed by other viral, bacterial and even fungal ligands. NKp46/Ncr1 also recognizes unknown self and tumor ligands. Here we describe the generation of a transgenic mouse where the Ncr1 gene is expressed in the Rosa locus, preceded by a floxed stop sequence allowing Ncr1/NKp46 expression in various tissues upon crossing with Cre transgenic mouse lines. Surprisingly, while several crossings were attempted, Ncr1 overexpression was successful only where cre recombinase expression was dependent on the Ncr1 promoter. Ncr1 overexpression in NK cells increased NK cell immunity in two hallmark Ncr1 related pathologies, influenza virus infection and B16 melanoma. These data suggest that increasing NK cell cytotoxicity by enforced NKp46/Ncr1 expression serves as a potential therapeutic opportunity for the treatment of various pathologies, and in immunotherapy.
Extreme tooth enlargement in a new Late Cretaceous rhabdodontid dinosaur from Southern France
Authors: Godefroit Pascal , Garcia Géraldine , Gomez Bernard , Stein Koen , Cincotta Aude , Lefèvre Ulysse , Valentin Xavier 
Published: October 2017

AbstractRhabdodontidae is a successful clade of ornithopod dinosaurs, characteristic of Late Cretaceous continental faunas in Europe. A new rhabdodontid from the late Campanian, of southern France, Matheronodon provincialis gen. et sp. nov., is characterized by the extreme enlargement of both its maxillary and dentary teeth, correlated to a drastic reduction in the number of maxillary teeth (4 per generation in MMS/VBN-02-102). The interalveolar septa on the maxilla are alternately present or resorbed ventrally so as to be able to lodge such enlarged teeth. The rhabdodontid dentition and masticatory apparatus were adapted for producing a strict and powerful shearing action, resembling a pair of scissors. With their relatively simple dentition, contrasting with the sophisticated dental batteries in contemporary hadrosaurids, Matheronodon and other rhabdodontids are tentatively interpreted as specialized consumers of tough plant parts rich in sclerenchyma fibers, such as Sabalites and Pandanites.
Alterations of regional homogeneity and functional connectivity in pituitary adenoma patients with visual impairment
Authors: Song Guidong , Qiu Jicheng , Li Chuzhong , Li Jiye , Gui Songbai , Zhu Haibo , Zhang Yazhuo 
Published: October 2017

AbstractEvidences have showed that the deprivation of vision can considerably alter the resting-state activity both within and beyond the visual cortices. However, the functional changes of the brain cortices related to partially vision-deprivation are still largely unknown. Using resting-state functional MR imaging, we quantitatively evaluated the regional homogeneity(ReHo) and functional connectivity(FC) changes between 25 pituitary adenoma patients with visual impairment and 25 healthy controls(HCs). Compared with HCs, PAs exhibited significant increased ReHo in the left superior occipital gyrus, bilateral middle occipital gyrus and reduced ReHo in the left inferior frontal gyrus and right middle temporal gyrus. PAs also showed decreased FC between vision-related area and higher-order cognitive brain areas. Furthermore, we identified that in the PAs group the FC between the left V1 and left V3 increased while the FC between left V2v and left V5 significantly decreased, the FC between left V4 area and the V3, V2d area increased. In our study, we identified that the ReHo and FC were altered between the vision-related cortices and other higher-order cognitive cortices along with disorganized functional connectivity within the visual system in PAs with visual impairment. These findings may provide important insights to understand the plasticity of visual network.
Differences in Tribological Behaviors upon Switching Fixed and Moving Materials of Tribo-pairs including Metal and Polymer
Authors: Xu Aijie , Tian Pengyi , Wen Shizhu , Guo Fei , Hu Yueqiang , Jia Wenpeng , Dong Conglin , Tian Yu 
Published: October 2017

AbstractThe coefficient of friction (COF) between two materials is usually believed to be an intrinsic property of the materials themselves. In this study, metals of stainless steel (304) and brass (H62), and polymers of polypropylene (PP) and polytetrafluoroethylene (PTFE) were tested on a standard ball-on-three-plates test machine. Significantly different tribological behaviors were observed when fixed and moving materials of tribo-pairs (metal/polymer) were switched. As an example, under the same applied load and rotating speed, the COF (0.49) between a rotating PP ball and three fixed H62 plates was approximately 2.3 times higher than that between switched materials of tribo-pairs. Meanwhile, the COF between H62 and PTFE was relatively stable. The unexpected tribological behaviors were ascribed to the thermal and mechanical properties of tribo-pairs. Theoretical analysis revealed that the differences in the maximum local temperature between switching the fixed and moving materials of tribo-pairs were consistent with the differences in the tested COF. This result indicated the precise prediction of the COF of two materials is complexcity, and that thermal and mechanical properties should be properly considered in designing tribo-pairs, because these properties may significantly affect tribological performance.
Primary Astrocytic Tumours and Paired Recurrences have Similar Biological Features in IDH1, TP53 and TERTp Mutation and MGMT, ATRX Loss
Authors: Li Xia , Wei Jie , Liu Yixiong , Li Peifeng , Fan Linni , Wang Yingmei , Li Mingyang , Zhao Danhui , Yu Zhou , Ye Jing , Guo Ying , Yan Qingguo , Guo Shuangping , Wang Zhe 
Published: October 2017

AbstractAstrocytic tumours are the most common type of primary malignant brain tumour. Most astrocytic tumours will recur at some point after surgery. Currently, the combination of radiotherapy and chemotherapy does not prevent the recurrence of astrocytic tumours. In this study, we investigated the consistency in isocitrate dehydrogenase 1 (IDH1), tumour protein p53 (TP53) and telomerase reverse transcriptase promoter (TERTp) mutations during astrocytic tumour recurrence. We also evaluated the protein loss of O-6-methylguanine-DNA methyltransferase (MGMT) and alpha-thalassemia/mental retardation, X-linked (ATRX) during disease recurrence. We then determined the prognostic significance of these findings in terms of progression-free survival (PFS) using Kaplan-Meier analysis and Cox regression models. Our results showed that in most cases,IDH1,TP53andTERTpmutation status and MGMT and ATRX protein expression levels were stable during recurrence, which may indicate that these alterations occurred early in astrocytic tumour development. Furthermore, inIDH1wild type group, the patients who were negative for MGMT and had a low Ki67 index showed a longer PFS. Therefore, we suggest thatIDH1mutation combined with MGMT expression level and Ki67 index might be an effective biomarker panel for evaluating the PFS of patients with astrocytic tumours.
A Unified Framework for Modeling Continuum and Rarefied Gas Flows
Authors: Xiao Hong , Tang Ke 
Published: October 2017

AbstractThe momentum and heat transport in rarefied gas flows is known to deviate from the classical laws of Navier and Fourier in Navier-Stokes-Fourier (NSF) equations. A more sophisticated Nonlinear Coupled Constitutive Model (NCCM) has been derived from the Boltzmann equation to describe gaseous and thermal transport both in continuum and rarefied gas flows. We first develop a unified numerical framework for modeling continuum and rarefied flows based on the NCCM model both in two and three dimensions. Special treatment is given to the complex highly nonlinear transport equations for non-conserved variables that arise from the high degree of thermal nonequilibrium. For verification and validation, we apply the present scheme to a stiff problem of hypersonic gas flows around a 2D cylinder, a 3D sphere, and the Apollo configuration both in continuum and rarefied situations. The results show that the present unified framework yields solutions that are in better agreement with the benchmark and experimental data than are the NSF results in all studied cases of rarefied problems. Good agreement is observed between the present study and the NSF results for continuum cases. The results show that this study provides a unified framework for modeling continuum and rarefied gas flows.
Controlled delivery and minimally invasive imaging of stem cells in the lung
Authors: Kim Jinho , Guenthart Brandon , O’Neill John D. , Dorrello N. Valerio , Bacchetta Matthew , Vunjak-Novakovic Gordana 
Published: October 2017

AbstractIntratracheal delivery of stem cells into injured or diseased lungs can provide a variety of therapeutic and immunomodulatory effects for the treatment of acute lung injury and chronic lung disease. While the efficacy of this approach depends on delivering the proper cell dosage into the target region of the airway, tracking and analysis of the cells have been challenging, largely due to the limited understanding of cell transport and lack of suitable cell monitoring techniques. We report on the transport and deposition of intratracheally delivered stem cells as well as strategies to modulate the number of cells (e.g., dose), topographic distribution, and region-specific delivery in small (rodent) and large (porcine and human) lungs. We also developed minimally invasive imaging techniques for real-time monitoring of intratracheally delivered cells. We propose that this approach can facilitate the implementation of patient-specific cells and lead to enhanced clinical outcomes in the treatment of lung disease with cell-based therapies.
Hepatitis virus infection and age-related cataract
Authors: Park Sangshin , Choi Nam-Kyong 
Published: October 2017

AbstractThis study was performed to investigate the relationships of hepatitis B (HBV) and hepatitis C virus (HCV) infection to age-related cataract, and to assess whether liver damage mediates the hepatitis-cataract association. This study analyzed data in the Korea National Health and Nutrition Examination Survey 2010–2012 on 10,037 participants aged ≥40 years. We performed mediation analysis to address the contribution of serum markers of liver damage, high aspartate (AST, >49.9 IU/L) and alanine aminotransferase (ALT, >56.1 IU/L), to the relationships of HBV and HCV infection to cataract. Odds ratios (ORs) for nuclear and any cataract with HBV infection were 1.09 [95% confidence interval (95CI) = 1.02–1.16] and 1.07 (95CI = 1.00–1.14), respectively, compared to HBV uninfection; ORs with HCV infection were 1.35 (95CI = 1.18–1.55) and 1.40 (95CI = 1.12–1.76), respectively. High AST completely mediated the HBV infection-any cataract association. The significant relationships of HCV infection with nuclear and any cataract were formed only by their direct effects, not by mediation effects of high AST or ALT. HBV and HCV infection was significantly associated with nuclear and any cataract. High AST significantly mediates the effects of HBV infections on any cataract outcome, but the associations of HCV infection with nuclear and any cataract were not mediated by high AST or ALT.
Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice
Authors: Fu Kequan , Miyamoto Yoshiaki , Otake Kazuya , Sumi Kazuyuki , Saika Eriko , Matsumura Shohei , Sato Naoki , Ueno Yuka , Seo Seunghee , Uno Kyosuke , Muramatsu Shin-ichi , Nitta Atsumi 
Published: October 2017

AbstractChronic exposure to methamphetamine causes adaptive changes in brain, which underlie dependence symptoms. We have found that the transmembrane protein 168 (TMEM168) is overexpressed in the nucleus accumbens of mice upon repeated methamphetamine administration. Here, we firstly demonstrate the inhibitory effect of TMEM168 on methamphetamine-induced behavioral changes in mice, and attempt to elucidate the mechanism of this inhibition. We overexpressed TMEM168 in the nucleus accumbens of mice by using an adeno-associated virus vector (NAc-TMEM mice). Methamphetamine-induced hyperlocomotion and conditioned place preference were attenuated in NAc-TMEM mice. Additionally, methamphetamine-induced extracellular dopamine elevation was suppressed in the nucleus accumbens of NAc-TMEM mice. Next, we identified extracellular matrix protein osteopontin as an interacting partner of TMEM168, by conducting immunoprecipitation in cultured COS-7 cells. TMEM168 overexpression in COS-7 cells induced the enhancement of extracellular and intracellular osteopontin. Similarly, osteopontin enhancement was also observed in the nucleus accumbens of NAc-TMEM mice, inin vivostudies. Furthermore, the infusion of osteopontin proteins into the nucleus accumbens of mice was found to inhibit methamphetamine-induced hyperlocomotion and conditioned place preference. Our studies suggest that the TMEM168-regulated osteopontin system is a novel target pathway for the therapy of methamphetamine dependence, via regulating the dopaminergic function in the nucleus accumbens.
Effects of predator lipids on dinoflagellate defence mechanisms - increased bioluminescence capacity
Authors: Lindström Jenny , Grebner Wiebke , Rigby Kristie , Selander Erik 
Published: October 2017

AbstractShort flashes of blue light (bioluminescence) from dinoflagellates can reduce copepod grazing of light-emitting cells. Other protective strategies against grazing are toxicity, reduced cell chain length and altered swimming patterns in different phytoplankton. Both toxicity and bioluminescence capacity in dinoflagellates decrease in copepod-free cultures, but toxin production can be restored in response to chemical alarm signals from copepods, copepodamides. Here we show that strains of the dinoflagellates Lingulodinium polyedra and Alexandrium tamarense, kept in culture for 14 and 9 years respectively, are capable of increasing their total bioluminescence capacity in response to copepodamides. The luminescence response to mechanical stimulation with air bubbles also increases significantly in L. polyedra after exposure to copepodamides. Effects on size, swimming speed and rate of change of direction in L. polyedra and A. tamarense were not detected, suggesting that post-encounter mechanisms such as bioluminescence and toxin production may constitute the dominating line of defence in these taxa. To our knowledge, this study provides the first evidence of changes in bioluminescence physiology as a response to chemical cues from natural enemies and emphasizes the importance of bioluminescence as an anti-grazing strategy.
Morphology and mechanics of fungal mycelium
Authors: Islam M. R. , Tudryn G. , Bucinell R. , Schadler L. , Picu R. C. 
Published: October 2017

AbstractWe study a unique biomaterial developed from fungal mycelium, the vegetative part and the root structure of fungi. Mycelium has a filamentous network structure with mechanics largely controlled by filament elasticity and branching, and network density. We report the morphological and mechanical characterization of mycelium through an integrated experimental and computational approach. The monotonic mechanical behavior of the mycelium is non-linear both in tension and compression. The material exhibits considerable strain hardening before rupture under tension, it mimics the open cell foam behavior under compression and exhibits hysteresis and the Mullins effect when subjected to cyclic loading. Based on our morphological characterization and experimental observations, we develop and validate a multiscale fiber network-based model for the mycelium which reproduces the tensile and compressive behavior of the material.
Potential impacts of climate change on habitat suitability for the Queensland fruit fly
Authors: Sultana Sabira , Baumgartner John B. , Dominiak Bernard C. , Royer Jane E. , Beaumont Linda J. 
Published: October 2017

AbstractAnthropogenic climate change is a major factor driving shifts in the distributions of pests and invasive species. The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Qfly), is the most economically damaging insect pest of Australia’s horticultural industry, and its management is a key priority for plant protection and biosecurity. Identifying the extent to which climate change may alter the distribution of suitable habitat for Qfly is important for the development and continuation of effective monitoring programs, phytosanitary measures, and management strategies. We used Maxent, a species distribution model, to map suitable habitat for Qfly under current climate, and six climate scenarios for 2030, 2050 and 2070. Our results highlight that south-western Australia, northern regions of the Northern Territory, eastern Queensland, and much of south-eastern Australia are currently suitable for Qfly. This includes southern Victoria and eastern Tasmania, which are currently free of breeding populations. There is substantial agreement across future climate scenarios that most areas currently suitable will remain so until at least 2070. Our projections provide an initial estimate of the potential exposure of Australia’s horticultural industry to Qfly as climate changes, highlighting the need for long-term vigilance across southern Australia to prevent further range expansion of this species.
Biomarker-indicated extent of oxidation of plant-derived organic carbon (OC) in relation to geomorphology in an arsenic contaminated Holocene aquifer, Cambodia
Authors: Magnone Daniel , Richards Laura A. , Polya David A. , Bryant Charlotte , Jones Merren , van Dongen Bart E. 
Published: October 2017

AbstractThe poisoning of rural populations in South and Southeast Asia due to high groundwater arsenic concentrations is one of the world’s largest ongoing natural disasters. It is important to consider environmental processes related to the release of geogenic arsenic, including geomorphological and organic geochemical processes. Arsenic is released from sediments when iron-oxide minerals, onto which arsenic is adsorbed or incorporated, react with organic carbon (OC) and the OC is oxidised. In this study we build a new geomorphological framework for Kandal Province, a highly studied arsenic affected region of Cambodia, and tie this into wider regional environmental change throughout the Holocene. Analyses shows that the concentration of OC in the sediments is strongly inversely correlated to grainsize. Furthermore, the type of OC is also related to grain size with the clay containing mostly (immature) plant derived OC and sand containing mostly thermally mature derived OC. Finally, analyses indicate that within the plant derived OC relative oxidation is strongly grouped by stratigraphy with the older bound OC more oxidised than younger OC.