bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the Elecsys syphilis immunoassay for routine screening of serum samples in China
Authors: Tao Chuanmin , Hao Xiaoke , Xu Wei , Zhang Jie , Pan Shiyang , Tao Zhihua , Li Xiaofei , Chen Junmei , Zhang Bingchang , Qiu Yurong , Wu Yanan , Ou Qishui , Huang Xianzhang , Wang Lanlan 
Published: August 2017

AbstractWe compared the performance of the Roche Diagnostics Elecsys immunoassay for the detection of Treponema pallidum specific antibodies in patient serum samples with that of the Abbott Laboratories Architect chemiluminescent microparticle immunoassay and the InTec and KHB enzyme-linked immunosorbent assays, which are commonly used in China. We tested 13,767 serum samples collected from 13 independent laboratories throughout China, which included samples from 999 previously confirmed syphilis cases and 158 ‘borderline’ samples previously identified using the Architect, InTec, and KHB tests. The Mikrogen Syphilis Immunoblot was used to confirm positive test results. The consistency between the four different assays was 100%. The sensitivity of Elecsys immunoassay was 100% versus 98.26% for Architect, 99.11% for InTec; and 98.56% for KHB. The specificity of the Elecsys immunoassay was 99.81% versus 99.74% for Architect; 99.93% versus 99.80% for InTec; and 99.85% versus 99.77% for KHB. For borderline samples, the Elecsys immunoassay yielded no false-negative results and fewer false-positive results, compared to the other tests. Considering the ease-of-use, automation, high speed, and high throughput capacity of the Elecsys assay, the higher sensitivity and specificity indicate it is superior for routine screening of serum samples for syphilis diagnosis.
Prevalence of hyperuricemia and its related risk factors among preschool children from China
Authors: Li Nan , Zhang Shuang , Li Weiqin , Wang Leishen , Liu Huikun , Li Wei , Zhang Tao , Liu Gongshu , Du Yuexin , Leng Junhong 
Published: August 2017

AbstractTo estimate the prevalence of hyperuricemia and its major risk factors among Chinese preschool children. A cross-sectional survey was carried out in six central urban districts of Tianjin from March to June 2015. A cluster sampling was employed to obtain a random sample of preschool children. A total of 4073 children aged from 3 to 6 years participated in this survey. Logistic regression was used to obtain odds ratios and 95% confidence intervals. The overall mean serum uric acid concentrations was 243.0 ± 53.2 μmol/L, corresponding to a mean concentrations of 247.3 ± 53.7 μmol/L among boys and 238.3 ± 52.4 μmol/L among girls. The overall prevalence of serum uric acid ≥ 310 μmol/L among children was 10.1%. Boys, obesity, diastolic blood pressure, and serum triglyceride concentrations were associated with the increased risk of hyperuricemia in univariable models, although the statistically significant association between diastolic blood pressure, serum triglyceride concentrations and hyperuricemia disappeared in multivariable models. The prevalence of hyperuricemia among children aged from 3 to 6 years was relatively high. Several metabolic syndrome components were associated with the risk of hyperuricemia.
Probing the Hydrogen Enhanced Near-Field Emission of ITO without a Vacuum-Gap
Authors: Poole Jacob L. , Yu Yang , Ohodnicki Paul R. 
Published: August 2017

AbstractElectromagnetic fields produced by thermal fluctuation can excite the near-field optical states, creating the potential for thermal radiation orders of magnitude greater than what is predicted by Plank’s blackbody theory. The typical schemes employed to probe the trapped electromagnetic energy of the near-field are with considerable technical challenges, suffering from scalability and high costs, hindering widespread use. A waveguide-based scheme relying on photon tunneling is presented as an alternate approach, as waveguides inherently provide a large density of channels for photons to tunnel to with the required k-vector matching and probability density overlap. The conducted experiments with a 10 nm indium tin oxide film, having plasmonic resonance in the 1500 nm wavelength range, show that the near-field EM radiation can be extracted to the far-field by establishing the mode of de-excitation to be that of photon tunneling to a nearby waveguide. Furthermore, it is also demonstrated that the thermally emitted energy is very sensitive to changes in the surface free electron density, a property that is unique to the near-field. In addition to the ease of implementation and scalability, the proposed waveguide-based extraction method does not require a vacuum-gap, which is a significant reduction in the required complexity.
Procedural complication and long term outcomes after alcohol septal ablation in patients with obstructive hypertrophic cardiomyopathy: data from China
Authors: An Shuo-yan , Yang Yin-jian , Hang Fei , Wang Zhi-min , Fan Chao-mei 
Published: August 2017

AbstractData on procedural complications and long term survival after alcohol septal ablation (ASA) in Chinese patients with obstructive hypertrophic cardiomyopathy (HOCM) are lacking. We aimed to investigate long-term survival of HOCM patients after ASA and compared to the non-obstructive hypertrophic cardiomyopathy(NOHCM). A total of 233 patients with HOCM and a peak pressure gradient of ≥50 mm Hg at rest or with provocation were consecutively enrolled from Fuwai Hospital in China between 2000 and 2012. Another 297 patients without left ventricular outflow tract obstruction were regarded as control group. Periprocedural mortality of ASA were low (0.89%). Periprocedural lethal ventricular arrhythmia occurred in 9 patients (4.0%). Alcohol volume (RR 1.44, 95% CI: 1.03–2.03, P = 0.034) and age ≤40 years old (RR 4.63, 95% CI: 1.07–20.0, P = 0.040) were independent predictors for periprocedural lethal ventricular arrhythmia. The 10- year overall survival was 94.6% in the ASA group, similar with 92.9% in the NOHCM group (P = 0.930). In conclusion, periprocedural mortality and complications were rare in ASA. Long term survival after ASA were satisfactory and comparable to NOHCM. Patients under 40 years old should be more cautious undergoing ASA, for these patients were more likely to endure lethal ventricular arrhythmia during periprocedural period of ASA.
Potential therapeutic targets for ALS: MIR206, MIR208b and MIR499 are modulated during disease progression in the skeletal muscle of patients
Authors: Di Pietro Lorena , Baranzini Mirko , Berardinelli Maria Grazia , Lattanzi Wanda , Monforte Mauro , Tasca Giorgio , Conte Amelia , Logroscino Giandomenico , Michetti Fabrizio , Ricci Enzo , Sabatelli Mario , Bernardini Camilla 
Published: August 2017

AbstractAmyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of motor neurons followed by muscle weakness, paralysis and death. The disease progression is extremely variable among patients, and reliable prognostic markers have not been identified. The aim of the study was to functionally characterize selected genes and microRNAs acting in the skeletal muscle of ALS patients, taking into account the duration and evolution of the disease, in order to obtain information regarding the muscle response to ALS progression. This prospective, longitudinal study enrolled 14 ALS patients and 24 age- and sex-matched healthy controls. Gene expression and histological analysis indicated an increase of MIR208B and MIR499 levels and the predominance of slow fibres, respectively, in the muscles of patients with a slower disease progression. A decreased expression of MIR206 and increased levels of HDAC4, during the progression of the disease were also observed. Taken together, our data suggest that the molecular signalling that regulates re-innervation and muscle regeneration is hampered during the progression of skeletal muscle impairment in ALS. This could provide precious hints towards defining prognostic protocols, and designing novel tailored therapeutic approaches, to improve ALS patients’ care and delay disease progression.
Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion
Authors: Durand Nisha , Bastea Ligia I. , Döppler Heike , Eiseler Tim , Storz Peter 
Published: August 2017

AbstractDependent on their cellular localization, Protein Kinase D (PKD) enzymes regulate different processes including Golgi transport, cell signaling and response to oxidative stress. The localization of PKD within cells is mediated by interaction with different lipid or protein binding partners. With the example of PKD2, we here show that phosphorylation events can also contribute to localization of subcellular pools of this kinase. Specifically, in the present study, we show that tyrosine phosphorylation of PKD2 at residue Y87 defines its localization to the focal adhesions and leads to activation. This phosphorylation occurs downstream of RhoA signaling and is mediated via Src. Moreover, mutation of this residue blocks PKD2’s interaction with Focal Adhesion Kinase (FAK). The presence and regulation of PKD2 at focal adhesions identifies a novel function for this kinase as a modulator of cell adhesion and migration.
Change in sensitivity to visual error in superior colliculus during saccade adaptation
Authors: Kojima Yoshiko , Soetedjo Robijanto 
Published: August 2017

AbstractSaccadic eye movements provide a valuable model to study the brain mechanisms underlying motor learning. If a target is displaced surreptitiously while a saccade is underway, the saccade appears to be in error. If the error persists gradual neuronal adjustments cause the eye movement again to land near the target. This saccade adaptation typically follows an exponential time course, i.e., adaptation speed slows as adaptation progresses, indicating that the sensitivity to error decreases during adaptation. Previous studies suggested that the superior colliculus (SC) sends error signals to drive saccade adaptation. The objective of this study is to test whether the SC error signal is related to the decrease in the error sensitivity during adaptation. We show here that the visual activity of SC neurons, which is induced by a constant visual error that drives adaptation, decreases during saccade adaptation. This decrease of sensitivity to visual error was not correlated with the changes of primary saccade amplitude. Therefore, a possible interpretation of this result is that the reduction of visual sensitivity of SC neurons contributes an error sensitivity signal that could help control the saccade adaptation process.
REST, a master regulator of neurogenesis, evolved under strong positive selection in humans and in non human primates
Authors: Mozzi Alessandra , Guerini Franca Rosa , Forni Diego , Costa Andrea Saul , Nemni Raffaello , Baglio Francesca , Cabinio Monia , Riva Stefania , Pontremoli Chiara , Clerici Mario , Sironi Manuela , Cagliani Rachele 
Published: August 2017

AbstractThe transcriptional repressor REST regulates many neuronal genes by binding RE1 motifs. About one third of human RE1s are recently evolved and specific to primates. As changes in the activity of a transcription factor reverberate on its downstream targets, we assessed whether REST displays fast evolutionary rates in primates. We show that REST was targeted by very strong positive selection during primate evolution. Positive selection was also evident in the human lineage, with six selected sites located in a region that surrounds a VNTR in exon 4. Analysis of expression data indicated that REST brain expression peaks during aging in humans but not in other primates. Because a REST coding variant (rs3796529) was previously associated with protection from hippocampal atrophy in elderly subjects with mild cognitive impairment (MCI), we analyzed a cohort of Alzheimer disease (AD) continuum patients. Genotyping of two coding variants (rs3796529 and rs2227902) located in the region surrounding the VNTR indicated a role for rs2227902 in modulation of hippocampal volume loss, indirectly confirming a role for REST in neuroprotection. Experimental studies will be instrumental to determine the functional effect of positively selected sites in REST and the role of REST variants in neuropreservation/neurodegeneration.
Application of color Doppler ultrasound combined with Doppler imaging artifacts in the diagnosis and estimate of congenital hypertrophic pyloric stenosis
Authors: Ma Suihong , Liu Jianhua , Zhang Youxiang , Yang Yuwen , Jin Hai , Ma Xiaomei , Wei Hongqin 
Published: August 2017

AbstractCongenital Hypertrophic Pyloric Stenosis (CHPS) is a disease condition that is caused as a result of pylorus wall hypertrophy and hyperplasia. In this study, we used color Doppler flow imaging (CDFI) and Doppler artifacts technique to observe the blood flow of hypertrophic pylorus tissue and the dynamic imaging of liquid passing through the pyloric canal in CHPS infants. 65 cases of CHPS infants and 50 infants without CHPS served as control group. We found that there were statistically significant differences between the blood flow grade of muscular layer and mucosal layer between CHPS and control infants, but no significant differences were observed in the same group. Doppler artifacts technique demonstrated the whole process of contrast agent flow through pyloric canal was directly observed in 35 of 65 subjects, and the internal diameter of the pyloric canal was 1.93 ± 0.33mm.Conclusion that CDFI combined with color Doppler artifacts technique was proved to be effective to observe the distribution feature and blood flow grade in each layer of pyloric canal in CHPS patients. This method provides the evidence for judging the degree of pyloric stenosis clinically, and furnishes the basis of therapy along with its clinical significance and good application value.
Copper Induced Conformational Changes of Tripeptide Monolayer Based Impedimetric Biosensor
Authors: Mervinetsky Evgeniy , Alshanski Israel , Hamo Yonatan , Sandonas Leonardo Medrano , Dianat Arezoo , Buchwald Jörg , Gutierrez Rafael , Cuniberti Gianaurelio , Hurevich Mattan , Yitzchaik Shlomo 
Published: August 2017

AbstractCopper ions play a major role in biological processes. Abnormal Cu2+ ions concentrations are associated with various diseases, hence, can be used as diagnostic target. Monitoring copper ion is currently performed by non-portable, expensive and complicated to use equipment. We present a label free and a highly sensitive electrochemical ion-detecting biosensor based on a Gly-Gly-His tripeptide layer that chelate with Cu2+ ions. The proposed sensing mechanism is that the chelation results in conformational changes in the peptide that forms a denser insulating layer that prevents RedOx species transfer to the surface. This chelation event was monitored using various electrochemical methods and surface chemistry analysis and supported by theoretical calculations. We propose a highly sensitive ion-detection biosensor that can detect Cu2+ ions in the pM range with high SNR parameter.
Impact of negative inotropic drugs on accuracy of diastolic stress echocardiography for evaluation of left ventricular filling pressure
Authors: Salem Joe-Elie , Laveau Florent , Ceccaldi Alexandre , Funck-Brentano Christian , Hulot Jean Sebastien , Mameri Amel , Barthelemy Olivier , Helft Gerard , Feuvre Claude Le , Isnard Richard , Hammoudi Nadjib 
Published: August 2017

AbstractThe ratio of early diastolic trans-mitral flow velocity to tissue-Doppler mitral annular early diastolic velocity (E/e′), and left ventricular end-diastolic pressure(LVEDP) have been shown to be correlated at rest, provided that patients are not on positive inotropic drugs. Data concerning the latter correlation during exercise stress are conflicting. Therefore, we investigated if use of negative inotropic drugs (NID), impacts the accuracy of E/e′ as a surrogate for LVEDP during low-level exercise. An exercise(50 watts) during cardiac invasive hemodynamic monitoring and an exercise echocardiography were performed prospectively within 24 hours in 54 patients (81%male, 62 ± 9years) with preserved LV Ejection-Fraction. Before exercise, the patients had scattered LVEDP (13.8 ± 5.8 mmHg) and septal E/e′ (8.7 ± 2.7). Half of them were on NID, mainly betablockers(n = 26). The correlation between septal-E/e′ and LVEDP was low for examinations performed at rest (r = 0.35,p = 0.01) with no significant impact of NID. For measurements performed at 50 Watts, NID had a significant impact on the association between septal-E/e′50 watts and LVEDP50 watts (β = −0.28,p = 0.03). Correlation between septal-E/e′50 watts and LVEDP50 watts persisted in patients on NID (r = 0.61,p = 0.001) while it disappeared in the group of patients with no NID (r = 0.15,p = 0.47). NID use is an important confounding factor to take into consideration when assessing exercise LVFP using stress E/e′ in patients with preserved LVEF.
The water kinetics of superabsorbent polymers during cement hydration and internal curing visualized and studied by NMR
Authors: Snoeck D. , Pel L. , De Belie N. 
Published: August 2017

AbstractSuperAbsorbent Polymers (SAPs) can be applied as an admixture in cementitious materials. As the polymers are able to swell, they will absorb part of the mixing water and can then release that water back towards the cementitious matrix for internal curing. This is interesting in terms of autogenous shrinkage mitigation as the internal relative humidity is maintained. The mechanism is theoretically described by the Powers and Brownyard model, but the kinetics and water release still remain subject of detailed investigation. This paper uses Nuclear Magnetic Resonance (NMR) to study the release of water from the superabsorbent polymers towards the cementitious matrix during cement hydration. The release of water by the SAPs is monitored as a function of time and degree of hydration. The internal humidity is also monitored in time by means of sensitive relative-humidity sensors.
Olfactory receptor 10J5 responding to α-cedrene regulates hepatic steatosis via the cAMP–PKA pathway
Authors: Tong Tao , Ryu Sang Eun , Min Yeojin , de March Claire A. , Bushdid Caroline , Golebiowski Jérôme , Moon Cheil , Park Taesun 
Published: August 2017

AbstractEctopic expression and functions of odorant receptors (ORs) in the human body have aroused much interest in the past decade. Mouse olfactory receptor 23 (MOR23, olfr16) and its human orthologue, OR10J5, have been found to be functionally expressed in several non-olfactory systems. Here, using MOR23- and OR10J5-expressing Hana3A cells, we identified α-cedrene, a natural compound that protects against hepatic steatosis in mice fed the high-fat diet, as a novel agonist of these receptors. In human hepatocytes, an RNA interference-mediated knockdown of OR10J5 increased intracellular lipid accumulation, along with upregulation of lipogenic genes and downregulation of genes related to fatty acid oxidation. α-Cedrene stimulation resulted in a significant reduction in lipid contents of human hepatocytes and reprogramming of metabolic signatures, which are mediated by OR10J5, as demonstrated by receptor knockdown experiments using RNA interference. Taken together, our findings show a crucial role of OR10J5 in the regulation of lipid accumulation in human hepatocytes.
Quantitative analysis of different nitrogen isotope labelled nitrates in paddy soil using mid-infrared attenuated total reflectance spectroscopy
Authors: Shao Yanqiu , Du Changwen , Zhou Jianmin , Ma Fei , Zhu Ying , Yang Kai , Tian Chao 
Published: August 2017

Nitrogen isotope labelled technology with mass spectroscopy is commonly used to trace the fate of agricultural N in the environment.
Africa’s next debt crisis: regulatory dilemmas and radical insights
Authors: Bassett Carolyn 
Published: August 2017

ABSTRACTA new African debt crisis appears imminent, which will have new features because several countries have recently introduced international sovereign bonds. Organisations such as the World Bank and the African Development Bank increasingly acknowledge the risk of such a crisis, but continue to prescribe debt-management strategies based on liberalisation and government spending cuts. Insights drawn from liberals favouring government regulation identify an alternative policy direction, while Marxist scholars raise serious concerns about the merits and implications of the bonds altogether. These alternative approaches have the potential to enrich inter-governmental policy discussions and potentially avert the looming new African debt crisis.
An L319F mutation in transmembrane region 3 (TM3) selectively reduces sensitivity to okaramine B of the Bombyx mori l-glutamate-gated chloride channel
Authors: Furutani Shogo , Okuhara Daiki , Hashimoto Anju , Ihara Makoto , Kai Kenji , Hayashi Hideo , Sattelle David B , Matsuda Kazuhiko 
Published: August 2017

Abstract
Okaramines produced by Penicillium simplicissimum AK-40 activate l-glutamate-gated chloride channels (GluCls) and thus paralyze insects. However, the okaramine binding site on insect GluCls is poorly understood. Sequence alignment shows that the equivalent of residue Leucine319 of the okaramine B sensitive Bombyx mori (B. mori) GluCl is a phenylalanine in the okaramine B insensitive B. mori γ-aminobutyric acid-gated chloride channel of the same species. This residue is located in the third transmembrane (TM3) region, a location which in a nematode GluCl is close to the ivermectin binding site. The B. mori GluCl containing the L319F mutation retained its sensitivity to l-glutamate, but responses to ivermectin were reduced and those to okaramine B were completely blocked.
The dualisation of unemployment compensation in emerging economies: Brazil, China and Russia
Authors: Ozkan Umut Riza 
Published: August 2017

AbstractThis article examines the development of a ‘dualised’ welfare regime –generous earnings-related unemployment compensation for ‘insiders’ andresidualneeds-based social assistance provision for ‘outsiders’ – in China and Brazil, which experienced impressive economic development in recent decades. It argues that such a welfare outcome can partly be accounted for by the ongoing influence of ‘insiders’, which was conditional upon the pace and nature of economic liberalisation reforms and their representation in institutional channels of social policy-making. It also demonstrates that the new social/unemployment assistance schemes for ‘outsiders’ emerged due to both governments’ fear of losing their power in politics; yet, these schemes were designed in a residual way since ‘outsiders’ did not possess the same political resources as ‘insiders’ did. The paper, moreover, draws from the Russian experience (a negative case) to demonstrate that such a dualised welfare outcome did not take place because the ‘insiders’ were weak, owing to a radical and orthodox liberalisation and they did not have access to institutional venues to influence social policy-making process.