bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bayesian Inference for the Calibration of Cohesive Zone Models of Dovetail Specimens From Laminated Composite Fan Blade
Authors: Tang Xu , Chen Yong 
Published: September 2024

Abstract
Composite fan blades are the preferred alternative for the fan stage of most advanced high bypass ratio turbofan engines. The dovetail part bears a significant centrifugal load, and its ability to safely bear this load is one of the key points of the multilevel “test pyramid” approach of compliance demonstration. Debonding between adjacent layers is the main damage mode of laminated composite fan blades. However, there is difficulty in measuring the as-manufactured interlaminar mechanical properties used in finite element models. In this study, tensile loading was applied to simulate the interacting centrifugal force and capture mixed-mode damage evolution. Structural responses and material damages were calibrated with measured tensile loads through Bayesian inversion, where interface and contact elements with distinct bilinear behavior were selected. Posterior probability distributions of maximum interface tractions and contact stresses were solved using Markov chain Monte Carlo (MCMC) sampler. Results indicated that the two bilinear cohesive material models had a capacity of predicting empirical means of longitudinal reaction forces as that in test considering additional discrepancy term (0.035 kN and 0.96 kN respectively), while they made an significant impact on the prediction of tensile load history especially when two delamination cracks initiated and propagated. Interface elements provided a higher matching quality in predicting loading history and capturing damage mechanism in association with in-plane progressive damage analysis. This calibrated parameter set could be functioned as benchmark in numerically determining the ultimate tensile load of dovetail elements and reducing the necessary number of physical tests at elemental length level.
Validation of Energy Saving From Cooperative Driving Automation via Vehicle-in-the-Loop Tests
Authors: Hyeon Eunjeong , Di Russo Miriam , Zhan Lu , Jeong Jongryeol , Kim Namdoo , Han Jihun , Misra Priyash , Stutenberg Kevin , Karbowski Dominik 
Published: September 2024

Abstract
This article presents an experimental validation of energy savings achieved through cooperative driving automation (CDA) measured by vehicle-in-the-loop (VIL) testing in car-following scenarios. The impacts of different CDA classes—from status sharing to prescriptive—on vehicle energy efficiency are explored. In the experiments, a plug-in hybrid electric vehicle runs on a chassis dynamometer integrated with simulation software that creates a virtual environment. Results indicate that when agreement-seeking cooperation operates with even a minimal number of vehicles, energy can be saved by up to 5% over human driving. Our findings highlight the considerable promise of CDA technologies for enhancing energy efficiency, especially fostering research on agreement-seeking cooperation.
Improving the Agreement Between the First Heart-Rate-Variability Threshold and the Gas-Exchange Threshold
Authors: Rogers Bruce , Fleitas-Paniagua Pablo R. , Murias Juan M. 
Published: September 2024

Purpose: The first heart-rate (HR) -variability (HRV) -derived threshold based on detrended fluctuation analysis alpha 1 (DFA a1) has shown inconsistent agreement with the gas-exchange threshold (GET). This study examined whether a custom method of computing the first HRV threshold (HRVT1) based on individual HRV characteristics would improve agreement. Methods: Fourteen participants underwent ramp incremental testing measuring gas-exchange variables and RR intervals. Comparisons were made between the oxygen consumption at the GET versus the at the standard DFA a1 = 0.75 (HRVT1s) or a custom value (HRVT1c) based on the DFA a1 midway between the maximum seen during the early ramp incremental and 0.5. Results: Mean values for GET versus HRVT1s and GETHR versus HRVT1sHR were statistically different (25.4 [3.3] vs 29.8 [6.8] mL·kg−1·min−1, P = .01, d = 0.80; 131 [11] vs 146 [22] beats·min−1, P = .005, d = 0.91). There were no statistical differences when using the HRVT1c (25.4 [3.3] vs 25.1 [5.7] mL·kg−1·min−1, P = .77, d = 0.08; 131 [11] vs 132 [17] beats·min−1, P = .65, d = 0.12). Equivalence between GET and HRVT1c was also verified. Mean maximal DFA a1 during the early ramp incremental was 1.52 (0.22) with mean HRVT1c of 1.01 (0.11). Pearson r correlation coefficients were between .67 and .70 for all GET to HRVT1 comparisons. The second HRV threshold and respiratory compensation point parameters showed agreement and correlations in line with prior studies. Conclusions: The HRVT1c showed stronger agreement to GET parameters than seen using the HRVT1s. It is recommended that evaluations of the HRVT1 consider this approach in determining the HR and at this threshold.
Ten-Year Evolution of World Swimming Trends for Different Performance Clusters: A Gaussian Model
Authors: Veiga Santiago , Grenouillat Alexia , Rodríguez-Adalia Luis , Zarzosa-Alonso Fernando , Pla Robin 
Published: September 2024

Purpose: To analyze the evolution of the world ranking in swimming over the last 10 years, with particular attention to the effects of COVID-19 on the different levels of participating athletes. Methods: The top 200 world-ranked entries in all swimming events (50-m pool) were collected from 2013 to 2022. A mathematical model (Gaussian model) was proposed to evaluate the ranking progression for different performance levels (clusters) according to distance, stroke, and gender. The model was applied both with and without the COVID season data. Results: Overall results indicated a general progression in world rankings over the last 10 years, except for the COVID season and the post-Olympic year(s), with peak results in the 2021 postpandemic (Olympic) year. The gender gap in World Aquatics points scoring has shown an increasing gap in favor of males since 2017, reaching 1.5% in 2022. The top 200 positions of world rankings were grouped into 3 different clusters defined by the 23.3%, 66.5%, and 100% of ranked male swimmers (or 31.5%, 72.5%, and 100% for females) and with average World Aquatics scores of 910 (12), 858 (10) and 816 (11) points (907 [13], 847 [11], and 802 [12] for females). The Gaussian model showed a gap averaging ∼21 to ∼36 points between performance curves with or without COVID season data, with larger gaps for female rankings and cluster-3 swimmers. Conclusions: These results suggest that, given the lower relative performance of female swimmers in the different clusters of world rankings, female events may provide an opportunity to enter international-level swimming.
Prospective Association Between 24-Hour Movement Behaviors and Fundamental Movement Skills in Chinese Preschoolers During the COVID-19 Pandemic: A Compositional and Reallocation Analysis
Authors: Song Huiqi , Lau Patrick W.C. , Wang Jing-Jing , Zhou Peng , Shi Lei 
Published: September 2024

Background: This prospective observation study explored the association between 24-hour movement behaviors and fundamental movement skills (FMS) in Chinese preschoolers during the COVID-19 pandemic. Methods: Four hundred and eighteen preschoolers (226 males; 4.0 [0.6] y old) from Zhuhai, China, completed the device-based physical activity (PA) and sedentary behavior measures, and sleep duration was reported by parents at baseline (October 2021 to December 2021). FMS was assessed using the Test of Gross Motor Development: Third Edition at 1-year follow-up (October 2022 to December 2022). The compositional analysis and isotemporal substitution were used. Results: Moderate to vigorous PA (MVPA) was positively associated with locomotor skills and ball skills (P < .05), and light physical activity was negatively associated with locomotor skills (P < .05) during the COVID-19 pandemic. FMS improvements were linked to the addition of MVPA at the expense of light physical activity, sedentary behavior, and sleep. The estimated detriments to FMS were larger in magnitude than the estimated benefits of time reallocation from MVPA to light physical activity, sedentary behavior, and sleep. Conclusions: This study provides evidence about 24-hour movement behaviors and FMS during the COVID-19 pandemic and highlights the importance of participating in MVPA to improve preschoolers’ FMS development during the COVID-19 era.
Spectinamide MBX-4888A exhibits favorable lesion and tissue distribution and promotes treatment shortening in advanced murine models of tuberculosis
Authors: Bauman Allison A. , Sarathy Jansy P. , Kaya Firat , Massoudi Lisa M. , Scherman Michael S. , Hastings Courtney , Liu Jiuyu , Xie Min , Brooks Elizabeth J. , Ramey Michelle E. , Jones Isabelle L. , Benedict Noalani D. , Maclaughlin Madelyn R. , Miller-Dawson Jake A. , Waidyarachchi Samanthi L. , Butler Michelle M. , Bowlin Terry L. , Zimmerman Matthew D. , Lenaerts Anne J. , Meibohm Bernd , Gonzalez-Juarrero Mercedes , Lyons Michael A. , Dartois Veronique , Lee Richard E. , Robertson Gregory T. 
Published: September 2024

ABSTRACT

The spectinamides are novel, narrow-spectrum semisynthetic analogs of spectinomycin, modified to avoid intrinsic efflux by
Mycobacterium tuberculosis
. Spectinamides, including lead MBX-4888A (Lee-1810), exhibit promising therapeutic profiles in mice, as single drugs and as partner agents with other anti-tuberculosis antibiotics including rifampin and/or pyrazinamide. Here, we show that MBX-4888A, given by injection with the front-line standard of care regimen, is treatment shortening in multiple murine tuberculosis infection models. The positive treatment responses to MBX-4888A combination therapy in multiple mouse models, including mice exhibiting advanced pulmonary disease, can be attributed to favorable distribution in tissues and lesions, retention in caseum, along with favorable effects with rifampin and pyrazinamide under conditions achieved in necrotic lesions. This study also provides an additional data point regarding the safety and tolerability of spectinamide MBX-4888A in long-term murine efficacy studies.
Rift Valley fever virus is able to cross the human blood–brain barrier
in vitro
by direct infection with no deleterious effects
Authors: Quellec Jordan , Piro-Megy Camille , Cannac Marion , Nisole Sébastien , Marty Florent H. , Gosselet Fabien , Shimizu Fumitaka , Kanda Takashi , Cêtre-Sossah Catherine , Salinas Sara 
Published: September 2024

ABSTRACT

Rift Valley fever (RVF) is a zoonotic arboviral disease that causes recurrent epidemics in Africa that may trigger fatal neurological disorders. However, the mechanisms of neuroinvasion by which the RVF virus (RVFV) reaches the human central nervous system (CNS) remain poorly characterized. In particular, it is not clear how RVFV is able to cross the human blood–brain barrier (hBBB), which is a neurovascular endothelium that protects the brain by regulating brain and blood exchanges. To explore these mechanisms, we used an
in vitro
hBBB model to mimic
in vivo
hBBB selectiveness and apicobasal polarity. Our results highlight the ability of RVFV to cross the hBBB by direct infection in a non-structural protein S (NSs)-independent but strain-dependent manner, leading to astrocyte and pericyte infections. Interestingly, RVFV infection did not induce hBBB disruption and was associated with progressive elimination of infected cells with no impairment of the tight junction protein scaffold and barrier function. Our work also shows that NSs, a well described RVFV virulence factor, limited the establishment of the hBBB-induced innate immune response and subsequent lymphocyte recruitment. These results provide
in vitro
confirmation of the ability of RVFV to reach human CNS by direct infection of the hBBB without altering its barrier function, and provide new directions to explore human RVFV neurovirulence and neuroinvasion mechanisms.

IMPORTANCE

The RVF virus (RVFV) is capable of infecting humans and inducing severe and fatal neurological disorders. Neuropathogenesis and human central nervous system (CNS) invasion mechanisms of RVFV are still unknown, with only historical studies of autopsy data from fatal human cases in the 1980s and exploration studies in rodent models. One of the gaps in understanding RVFV human pathogenesis is how RVFV is able to cross the blood–brain barrier (BBB) in order to reach the human CNS. For the first time, we show that RVFV is able to directly infect cells of the human BBB
in vitro
to release viral particles into the human CNS, a well-characterized neuroinvasion mechanism of pathogens. Furthermore, we demonstrate strain-dependent variability of this neuroinvasion mechanism, identifying possible viral properties that could be explored to prevent neurological disorders during RVFV outbreaks.
Draft genome sequence of
Aspergillus ochraceus
VKM-F4104D (L-1) producing peptidase activator of protein C
Authors: Shestakova Anna , Surkova Daria , Fatkulin Artem , Osmolovskiy Alexander , Popova Elizaveta 
Published: September 2024

ABSTRACT

Aspergillus ochraceus
VKM-F4104D (L-1) is a saprotrophic fungus isolated from buried soils of Phanagoria (Russia). This strain is known as a producer of the fibrinolytic peptidase-activating plasma protein C. We have sequenced and assembled its genome for a more detailed understanding of the fungus’ physiology and encoded peptidases.
Complete genome sequence of white sturgeon herpesvirus 2 isolated from farmed white sturgeon (
Acipenser transmontanus
)
Authors: Doszpoly Andor , Subramaniam Kuttichantran , Kerr Karen , Davison Andrew J. , Waltzek Thomas B. 
Published: September 2024

ABSTRACT

The complete genome sequence of white sturgeon herpesvirus 2 (strain UC Davis) was determined. Comparative genomic analyses confirmed the classification of this virus in the species
Ictavirus acipenseridallo2
in the family
Alloherpesviridae
.
Improved draft genome of
Ignatzschineria larvae
DSM 13226 via hybrid assembly
Authors: Cantrell Emily G. , Burcham Zachary M. 
Published: September 2024

ABSTRACT

Ignatzschineria larvae
is studied for its role in decomposition and disease ecology; however, the type strain reference genome remains fragmented. The current reference genome consists of 61 contigs calculated at 82.18% complete with 10.98% contamination. Here, we announce the hybrid genome assembly as an improved single contig.
Genome sequence of the New Zealand cheese isolate and candidate probiotic strain
Lactiplantibacillus plantarum
FNZ042
Authors: de Almeida Eduardo L. , O’Toole Paul W. , Bassett Shalome A. 
Published: September 2024

ABSTRACT

The complete genome sequence of the candidate probiotic strain
Lactiplantibacillus plantarum
FNZ042 was determined using a hybrid genome assembly comprising data from Illumina and PacBio sequencing platforms. The genome assembly comprised 3,265,637 bp, including the complete circular chromosome and three circular plasmids.
Complete genome sequences of four representative
Corynebacterium belfantii
strains
Authors: Peng Yanhui , Fueston Heather , Irfan Muhammad , Hammond John , Morales Diego , Ju Hong , Bentz Meghan L. , Heuser James , Burroughs Mark , Tondella Maria Lucia , Weigand Michael R. 
Published: September 2024

ABSTRACT

This report describes the complete genome sequence assemblies from four representative isolates of the human pathogen
Corynebacterium belfantii
. These data provide necessary references to aid accurate sequence-based species discrimination among closely related
Corynebacterium
spp. pathogens.
Whole-genome sequences of vaginal isolates
Lactobacillus crispatus
CECT30647 and
Lactobacillus gasseri
CECT30648
Authors: Huedo Pol , Perez Marta , Armengol Eva , Del Casale Antonio , Campedelli Ilenia , Espadaler-Mazo Jordi 
Published: September 2024

ABSTRACT

We present the whole-genome sequences of
Lactobacillus crispatus
CECT30647 and
Lactobacillus gasseri
CECT30648, two vaginal isolates with probiotic potential.
Complete genome sequence of
Luteolibacter
sp. strain Populi, a member of phylum Verrucomicrobiota isolated from the
Populus trichocarpa
rhizosphere
Authors: Imai Sora , Allen Benjamin , Hochanadel Leah H. , Alexander William G. , Cottingham Robert W. , Schadt Christopher W. , Pelletier Dale A. , Podar Mircea 
Published: September 2024

ABSTRACT

Luteolibacter
sp. strain Populi is a bacterium from the phylum Verrucomicrobiota, isolated from the rhizosphere of a black cottonwood tree,
Populus trichocarpa
, from the Cascade mountains in Washington. Its 6.6-Mb chromosome was completely sequenced using Oxford Nanopore long-read sequencing and is predicted to encode 5,301 proteins and 60 RNAs.
Complete genome sequence of
Mediterraneibacter gnavus
strain RI1, isolated from human feces
Authors: Ichimura Ryoha , Tanaka Kazuki , Nakato Gaku , Fukuda Shinji , Arakawa Kazuharu 
Published: September 2024

ABSTRACT

We report the complete genome sequence of
Mediterraneibacter gnavus
strain RI1, a Gram-positive anaerobic gut microbe isolated from human feces. The complete circular genome has a genome size of 3.25  Mb, with a G+C content of 42.6%.
Draft genome sequence of
Gordonia
sp. ABKF26, a potential petroleum, plastic, and rubber-degrading bacterium, isolated from St. Albans Bay, VT, USA
Authors: Felix Kermisha , Barkley Ashley , Myers Luke , Ankrah Nana Y. D. 
Published: September 2024

ABSTRACT

Here, we present the draft genome sequence of
Gordonia
sp. ABKF26, a potential petroleum, plastic, and rubber degrading bacterium isolated from Lake Champlain. The assembled genome comprises a 6.0-Mb chromosome with a GC content of 67.8%.
Gordonia
sp. ABKF26 is predicted to be lysogenized by one intact 52.2 Kbp prophage.
Genome sequences of
Mycobacterium
sp. Elmwood and accompanying phage, isolated from a public swimming pool in Nebraska
Authors: Hernandez-Velazquez Daniela , Vasquez Madelynn K. , Petre Rana , Kyndt John A. 
Published: September 2024

ABSTRACT

Genome sequencing of a non-tuberculosis
Mycobacterium
species, isolated from a public pool, shows that the genome contains several genes for antibiotic resistance and anti-phage defense, which are absent from other related
Mycobacteria
. Metagenomic binning also provided the genome of the accompanying phage, which is distinct from other mycobacterial phages.
Comparative genome analysis of cluster EF bacteriophages Ajin and OverHedge isolated from soil in Tennessee
Authors: Markov Sergei A. , Atuahene Paul Y. , Barnes Clayton W. , Butler Taquerra , Cooper Stephan D. , Covel Emma M. , Diaz Kayla R. , Gadson Dalon , Holt Anna C. , Litchfield Maegan A. , Nefe Alexis J. , Ogbu Comfort E. , Rupp Kiara M. , Simpson Falisaty , Wood Elyse 
Published: September 2024

ABSTRACT

Bacteriophages Ajin and OverHedge were isolated from soil in Tennessee using the bacterium
Microbacterium foliorum
. Ajin and OverHedge (cluster EF) have a genome of 56,993 bp and 56,559 bp, containing 86 and 81 predicted genes, respectively. The Ajin genome has unique genes, phosphatase and glycosyltransferase, compared to the OverHedge.
Seasonal dynamics of
Haemaphysalis
tick species as SFTSV vectors in South Korea
Authors: Jang Hyunwoo , Casel Mark Anthony B. , Jang Seung-gyu , Choi Jeong Ho , Gil Juryeon , Rollon Rare , Cheun So youn , Kim Young-Il , Song Min Suk , Choi Young Ki 
Published: September 2024

ABSTRACT

Ticks pose a significant public health threat due to their ability to transmit various pathogens, including emerging tick-borne diseases. This study conducted a comprehensive surveillance of
Haemaphysalis
tick species and their severe fever with thrombocytopenia syndrome virus (SFTSV) infection rates in South Korea throughout the year 2023, from January to December. To ensure accurate and rapid identification of the prevalent
Haemaphysalis
tick species in South Korea, we designed PCR primer sets targeting the ITS1 gene, specifically distinguishing
Haemaphysalis longicornis
from
Haemaphysalis flava
. Among the 10,343 ticks collected from wild animals,
H. longicornis
constituted the majority, accounting for 65.5% (6,784/10,343 ticks), followed by
H. flava
at 33.8% (3,491/10,343 ticks), and
Ixodes nipponensis
at 0.7% (68/10,343 ticks). These identified ticks were then categorized into 811 pools, with 63 pools testing positive for SFTSV. Remarkably, the prevalence of SFTSV-positive
H. longicornis
ticks peaked during the summer months, aligning with heightened human outdoor activities and, consequently, an increased risk of human exposure. Conversely, it is noteworthy that
H. flava
exhibited a higher prevalence during the winter season, reaching its peak in January, with an SFTSV minimum infection rate similar to that of
H. longicornis
. These findings underscore the year-round presence of
Haemaphysalis
ticks as potential vectors for SFTSV, extending the temporal window for potential human exposure. Consequently, these results emphasize the necessity for active and continuous field surveillance to comprehensively understand and mitigate the public health risks associated with these tick-borne pathogens.

IMPORTANCE

To date, the majority of tick surveillance studies have primarily focused on warmer seasons, which are considered optimal periods for ticks to actively seek hosts and transmit pathogens through blood-feeding activities. Consequently, tick species active during winter have often been overlooked, leading to an underestimation of their significance in transmitting severe fever with thrombocytopenia syndrome virus (SFTSV). In this study, we aimed to examine year-round tick prevalence with SFTSV and illuminate the role of the winter-dominant species,
Haemaphysalis flav
a, in South Korea. Through rigorous identification facilitated by a primer set designed specifically for this purpose, we emphasize that
H. flava
, a competent vector species, harbors SFTSV in the winter season, thereby acting as an overwintering reservoir for the virus. This phenomenon may contribute to a higher infection rate among ticks in the following year.