bluuebunny/crossref_metadata_2025_split huggingface dataset:
Setdb1 regulates proper differentiation of adult intestinal stem cells via restraining permissive chromatin structure and transcriptional variability
Authors: Botskaris Dimitris , Deligiannis Ioannis K. , Peraki Ioanna , Kontaki Haroula , Stagaki Marianna , Lavigne Matthieu D. , Martinez-Jimenez Celia P. , Talianidis Iannis 
Published: October 2024

AbstractThe histone methylase Setdb1 plays a pivotal role in embryonic stem cell maintenance and developmental lineage specification. However, its function in adult stem cells remains elusive. Here we show that conditional inactivation ofSetdb1in Lgr5+intestinal stem cells alters the transcriptional programs of the progeny cell types and results in increased cell-to-cell transcriptional variability. Loss of Setdb1 blocked differentiation towards the absorptive enterocyte lineage, while the secretory cell types were only marginally affected due to the activation of alternative developmental trajectories.Setdb1inactivation did not alter H3K9 methylation at large heterochromatin domains but led to reduced localized modifications at islands of gene-rich areas around transposase-accessible chromatin regions, which doubled in number, became broader and more heterogeneous in size. The results demonstrate that Setdb1 regulates intestinal stem cell differentiation by fine-tuning chromatin accessibility in open euchromatin regions thereby controlling transcriptional variability between cells.
Determination ofPseudomonas aeruginosaMexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes
Authors: Kavanaugh Logan G. , Hariharan Shraddha M. , Conn Graeme L. 
Published: October 2024

ABSTRACTDefining the substrates of Resistance-Nodulation-Division (RND) systems is often complicated by the presence of multiple systems in a single bacterial species. Using a major efflux knockout strain, we developed aPseudomonas aeruginosasystem that enables controlled expression of MexXY-OprM in the absence of background efflux complexes with overlapping substrate profiles. This system identified three groups of potential substrates: substrates, partial substrates, and nonsubstrates, and defined trimethoprim as a new substrate for MexXY-OprM.
The genital tract microbiota of IVF patients and oocyte donors: a comparative analysis
Authors: Haahr Thor , Humaidan Peter , Bernabeu Andrea , Lledo Belen , Plummer Erica L , Bradshaw Catriona S , Ingham Anna Cäcilia , Jensen Jørgen Skov 
Published: October 2024

AbstractRecent studies have observed an association between genital tract microbiota and reproductive outcomes in patients undergoing in vitro fertilization (IVF). This finding could be caused by ascending dysbiosis from the vagina to the endometrium. The main objective of this study was to compare the microbiota and total bacterial load in the vagina, cervix and endometrium of IVF patients and oocyte donors. Thus, a total of two cohorts were explored: IVF patients (N=27) and healthy oocyte donors (N=26). The microbiota was investigated using 16S rRNA gene sequencing of the V3-V4 hypervariable regions, as well as qPCR for common vaginal species and total bacterial load. The study used a transcervical sampling approach for the endometrial microbiota samples.The highest bacterial load was seen in the vagina (median 2.6*109; 95% CI 9.7*108- 7.1*109; 16S rRNA gene copies/swab), followed by the cervix (median 9.0*107; 95% CI 3.3*107- 2.4*108; 16S rRNA gene copies/swab) and the endometrium (median 8.6*106; 95%CI 3.1*106- 2.4*107; 16S rRNA gene copies/swab). Only 1% of the observed variation between genital tract samples was explained by patient group (IVF patient or oocyte donor) and less than 1% of the variation between genital tract samples was explained by the anatomical site. Although, findings could be impacted by the transcervical sampling approach, 16S rRNA gene sequencing as well as species-specific qPCR showed a similar microbiota composition present in the cervix and the endometrium when compared to the vaginal microbiota.
Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows
Authors: van der Hoeven Leander , Lechner Maico , Hernandez-Rollan Cristina , Batth Tanveer S. , Olsen Jesper V. 
Published: October 2024

AbstractThis study presents a comparative analysis of three LysC homologues from Achromobacter lyticus, Pseudomonas aeruginosa, and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture (PAC) workflow with HeLa cell lysate, we assessed the enzymes’ cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three homologues exhbihited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with its superior peptide identification, digestion efficiency, and protein coverage, especially at short digestion times. Combination of A. lyticus LysC and trypsin demonstrated that importance of LysC for signifancanlty minimizing missed cleavage rates in tryptic digests. This study underscores A. lyticus LysC’s potential as an optimal choice for enhancing mass spectrometry-based proteomics.
Advanced deep-tissue imaging and manipulation enabled by biliverdin reductase knockout
Authors: Kasatkina Ludmila A. , Ma Chenshuo , Sheng Huaxin , Lowerison Matthew , Menozzi Luca , Baloban Mikhail , Tang Yuqi , Xu Yirui , Humayun Lucas , Vu Tri , Song Pengfei , Yao Junjie , Verkhusha Vladislav V. 
Published: October 2024

ABSTRACTWe developed near-infrared (NIR) photoacoustic and fluorescence probes, as well as optogenetic tools from bacteriophytochromes, and enhanced their performance using biliverdin reductase-A knock-out model (Blvra-/-). Blvra-/- elevates endogenous heme-derived biliverdin chromophore for bacteriophytochrome-derived NIR constructs. Consequently, light-controlled transcription with IsPadC-based optogenetic tool improved up to 25-fold compared to wild-type cells, with 100-fold activation in Blvra-/- neurons.In vivo, light-induced insulin production in Blvra-/- reduced blood glucose in diabetes by ∼60%, indicating high potential for optogenetic therapy. Using 3D photoacoustic, ultrasound, and two-photon fluorescence imaging, we overcame depth limitations of recording NIR probes. We achieved simultaneous photoacoustic imaging of DrBphP in neurons and super-resolution ultrasound localization microscopy of blood vessels ∼7 mm deep in the brain, with intact scalp and skull. Two-photon microscopy provided cell-level resolution of miRFP720-expressing neurons ∼2.2 mm deep. Blvra-/- significantly enhances efficacy of biliverdin-dependent NIR systems, making it promising platform for interrogation and manipulation of biological processes.
Dynamic characteristics of perforated plates with elastically restrained edges under impact loading
Authors: Wang Chen , Liu Rongqiang 
Published: October 2024

Purpose The purpose of this paper is to develop an effective method for predicting the dynamic ultimate bearing capacity of porous plates, and to analyse the influence of various geometric and boundary parameters on the ultimate bearing capacity of the plates.Design/methodology/approach The goal of this paper is to propose a generalized constrained differential quadrature method to investigate the dynamic ultimate bearing capacity of perforated plates with elastically restrained edges in which the perforated plate is modelled as the assembly of continuous plate elements using the penalty function method.Findings The effects of size and location of the cut-out, aspect ratio of the cut-out, elastic restrained edge stiffness and compression–shear load on the dynamic ultimate bearing capacity of plates are examined and discussed in detail.Originality/value A fast method for calculating the dynamic ultimate bearing capacity of perforated plate structures was proposed, and the influence of various geometric and boundary parameters on their ultimate bearing capacity was discussed, which is of great significance for the initial design of engineering structures.
Navigating the digital beat: a review of social media as a public engagement tool in policing
Authors: Ralph Liam , Elliott Ian C. , Murphy Joanne , Glennon Russ 
Published: October 2024

PurposeThis article explores the changing nature of social media use as a public engagement tool by police services.Design/methodology/approachA comprehensive review is conducted of academic studies from criminology and policing journals. Sources are identified from key academic databases and are analysed in relation to three decades (2000–2009, 2010–2019 and 2020 to present) to show how the use of social media has changed over time.FindingsThe way in which social media is used in policing has changed considerably over time. From initial enthusiasm, it is found that there is growing scepticism in the use of social media as a public engagement tool. After an initial proliferation in use, there is then increasing consolidation and control in response to concerns about reputational risk.Research limitations/implicationsThe research highlights underlying dynamics of engagement and retrenchment, which offer important insights for how we understand public engagement and value creation in policing and emergency services more generally. We draw on academic research from English-language academic journals, but we have endeavoured to include research from the broadest possible range of countries.Practical implicationsThis study demonstrates how the police and other public services must respond to the growing use of social media by the public to maximise value creation whilst minimising the threats that come from potential value destruction.Originality/valueThis study is the first to comprehensively review the policing and criminology literature related to social media and to apply a public engagement lens to this analysis.
Friendshoring: how geopolitical tensions affect foreign sourcing, supply base complexity, and sub-tier supplier sharing
Authors: Charpin Remi , Cousineau Martin 
Published: October 2024

PurposeThis paper examines the influence of geopolitical tensions—operationalized as political divergence between governments—on firms’ foreign supply bases and the resulting effects on supply base complexity and sub-tier supplier sharing.Design/methodology/approachThe authors conduct panel data regression analyses over the period 2003–2019 to investigate whether political divergence affects foreign supply bases for 2,858 US firms sourcing from 99 countries and to examine how political divergence exposure impacts the supply network structures of 853 US firms.FindingsFirms reduce their supply bases in countries exposed to heightened geopolitical tensions. These supply chain adjustments are associated with increased supply base complexity and greater sub-tier supplier sharing.Originality/valueThis study highlights the importance of state relations in global supply chain reconfiguration. Political divergence between governments provides a dual-view of political risk (i.e. buyer–supplier countries), which can help firms anticipate geopolitical disruptions. While reducing supply bases in foreign countries facing heightened geopolitical tensions is intended to mitigate disruptions, these supply base adjustments are linked to increased supply base complexity and sub-tier supplier sharing, thereby exposing firms to other types of supply disruptions. Additionally, this research contributes to understanding the effects of geopolitical tensions on supply base complexity through the lenses of transaction cost economics and resource dependence theory.
The influence of project-based learning on engineering students’ academic and career motivation
Authors: Deng Yanyao , Shi Chao 
Published: October 2024

PurposeThis study explored students’ variations of insightful academic and career motivation variations of learning and working on defense manufacturing engineering (DME) before and after a project-based program (PBL).Design/methodology/approachInterview was adopted to collect students’ insights. The first-round interview was completed within the first week of this summer internship, and the second-round interview was conducted during the final week of this summer internship. Thematic and content analysis were used to explore the factors that affect students’ perception, understanding and attitude to DME before and after the summer internship.FindingsThis paper discloses the improved level of learning and working motivation at the beginning of the project, but with a decrease in the motivation level of working in the DME area at the end of the program. These students showed a continued motivation of interests in pursuing more knowledge in the field. This PBL didn’t bring significant improvement on students’ career motivation for working in the field of DME. However, it demonstrated a highly positive effect on enhance students’ academic motivation (AM) for learning knowledge in the field. Moreover, the students’ concerns and perspectives on this PBL were also explored, which is displayed in the study.Originality/valueThe project was done with support from the US Department of Defense (DoD) (No: N00014-19-1-2728). These findings are investigated from the project.
The failure of passionate entrepreneurs to persist: a self-regulatory approach
Authors: Yang Hongtao , Xie Xin , Zhou Hanhui 
Published: October 2024

PurposeEntrepreneurial passion is constantly considered a core driver of the entrepreneurial process. In reality, however, many passionate entrepreneurs still fail to persist in their ventures. Whether entrepreneurial passion negatively affects entrepreneurial persistence is not well known. The current study explores how entrepreneurial passion inhibits entrepreneurial persistence based on self-regulation theory. The moderating role of the perceived entrepreneurial institutional environment on the relationship is further investigated.Design/methodology/approachThe study collected data from 200 entrepreneurs using a two-stage questionnaire. In the first stage, demographic information was obtained from the respondents, and their entrepreneurial passion, self-regulatory assessment mode and perceived entrepreneurial institutional environment were measured. Three months later, in the second stage, entrepreneurial persistence was evaluated.FindingsThe results show that entrepreneurial passion has a positive effect on self-regulatory assessment mode. Further, self-regulatory assessment mode negatively impacts entrepreneurial persistence and mediates the negative relationship between entrepreneurial passion and persistence. In addition, the greater the perceived level of the entrepreneurial institutional environment is, the weaker the positive association between entrepreneurial passion and self-regulatory assessment mode and the weaker the mediating effect of self-regulatory assessment mode on the relationship between entrepreneurial passion and entrepreneurial persistence and vice versa.Originality/valueThis paper clarifies the unknown negative effects of entrepreneurial passion, contributes to the theoretical relationship between the constructs of entrepreneurial passion and persistence and provides insights for decision-making by entrepreneurs, government and venture capital institutions.
Value and appraisal: human resource management practices and voice behaviors
Authors: Nguyen Thuy Chinh , Tran Huy Phuong 
Published: October 2024

PurposeEmployee voice is considered an important driver of organizational success. Management practices in the form of high-performance work practices (HPWPs) are expected to influence promotive and prohibitive voices. In addition, employee appraisal of HPWPs as a challenge or hindrance stressor is supposed to mediate the relationships. Finally, the self-direction value moderates the indirect effects of HPWPs on voice behaviors through employee appraisal.Design/methodology/approachA quantitative research approach was adopted with data collected from 315 subordinate supervisor dyads from 12 business organizations in Vietnam. This research uses partial least square structural equation modeling (PLS-SEM) to test the hypotheses.FindingsResults indicate that HPWPs are significantly associated with both types of employee voice behaviors. Challenge appraisal partially mediates the impact of HPWPs on promotive voice, while hindrance appraisal acts as a partial mediation in the HPWPs-prohibitive voice linkage. Self-direction value moderates the link between HPWPs and prohibitive voice.Research limitations/implicationsResults show that HPWPs may have both productive and harmful effects on employees. Depending on how the employees appraise HPWPs, they develop different coping strategies by raising their voices. HPWP-implementing organizations should pay attention to the employees’ values in this process.Originality/valueThis study investigates the relationship between HPWPs and employee voice based on the transactional theory of stress and coping. The mediating role of employee appraisal represents a new contribution. Finally, the role of self-direction value in the voice literature has not been widely evaluated in previous research.
Social mobility as a driver of employee silence in India: toward a contextualized understanding of silence in emerging markets
Authors: Knoll Michael , Bhattacharjee Anindo , Vandekerckhove Wim 
Published: October 2024

PurposeThis paper aims to explore how the context in a dynamically developing country affects employee silence in India.Design/methodology/approachQualitative design involving semi-structured interviews with employees and managers from different sectors in the Mumbai and Delhi areas. An abductive approach was used to analyze the data.FindingsTwo types of social mobility concerns – advancement aspiration and fear of social decline – emerged as salient drivers of silence and can be attributed to a volatile job market, social status markers, transferability of qualification, someone’s socio-economic situation and the overall economic situation. Pathways were specified from social mobility concerns to silence tendencies that are motivated by both low approach and high avoidance.Research limitations/implicationsSocial mobility as a specific factor in the Indian distal context and as a characteristic of emerging markets can motivate silence while organization-related concepts like job satisfaction or commitment may have less predictive value. Propositions that were derived from the interview study need to be validated by deductive research. Generalizability of Indian findings across other emergent markets needs to be shown.Originality/valueTo the organizational behavior (OB) scholarship on silence, this research contributes by identifying antecedents of silence that are situated beyond the organizational boundaries challenging the dominant role of established factors at the team- and organizational level. To the human resource management/employment relations (HRM/ER) scholarship, this research contributes by theorizing psychological processes that link environmental factors to silence behaviors.
Does job gamification perception promote the job involvement of gig workers? The role of cognitive assessment and overwork—evidence from Chinese delivery platforms
Authors: Wei Wei , Wang Xiaoyu , Han Siyi , Xiong Ailun 
Published: October 2024

PurposeThis paper takes the gig workers in Chinese delivery platform as the research object and adopts a questionnaire survey to explore the complex influence of job gamification perception on the job involvement of gig workers, via the mediating role of cognitive assessment and moderating role of overwork, in order to provide research and data support for the development of platform gamification.Design/methodology/approachThe study conducted a three-wave online questionnaire survey to obtain 300 final samples from Chinese delivery platforms. Hypotheses were tested hierarchical regression and bootstrap methods.FindingsDrawing on cognitive appraisal theory, we observed an inverted U-shaped relationship of gig workers between job gamification perception and job involvement. The mediating role of cognitive assessment and moderating role of overwork were also considered. Both challenge and threat assessment mediate the relationship between job gamification perception and job involvement. Direct effect of job gamification perception on job involvement and indirect effect of job gamification perception on cognitive assessment are moderated by overwork.Originality/valueIn the past, the research on job gamification mostly focused on the traditional forms of employment, but this study focuses on the new forms of employment and from the perspective of individual self-perception, explores the influence of job gamification perception on the job involvement of gig workers in Chinese delivery platform and investigated the dialectical role of job gamification perception. The findings enrich the literature and theoretical research on job gamification perception and job involvement and provide new references and perspectives for management practice.
Leaf herbivory causes abortion of selfed seeds in Rhododendron kaempferi var. kaempferi
Authors: Takahashi Keigo , Kudo Gaku 
Published: October 2024

AbstractPlant reproduction is often inhibited by herbivorous leaf damage, which affects the resource pool allocated for reproduction. Rhododendron kaempferi var. kaempferi is a semi‐evergreen shrub widely distributed in Japan, and its leaves are occasionally damaged by sawfly larvae (Arge similis). This species is partially self‐compatible, but its selfed seedlings suffer from inbreeding depression. Our study aimed to clarify (1) the magnitude of herbivorous damage under natural conditions, (2) the effects of foliage damage on seed number and mass, and (3) the possibility of selective abortion of selfed seeds to maintain outcrossed seed production under resource limitation. In the 2022 survey, 2 of the 20 ramets observed were collectively parasitized by sawfly larvae, and the most damaged ramet had a 59.6% foliage loss. In contrast, the herbivorous damage in 2023 was consistently low throughout the growing season, averaging a 3.2% loss. Leaf excision reduced the seed number per fruit (i.e., low seed‐set rate) but did not affect the individual seed mass. The outcrossing rate of the excision treatment (81.7%) was significantly higher than that of the control (72.0%). The values of seed‐set rate × outcrossing rate were 0.172 for the excision treatment and 0.171 for the control, nearly equal. Our results indicated that (1) sawfly larvae damage was concentrated on a few specific plants, (2) intense foliage damage reduced seed number but not seed mass, and (3) R. kaempferi mitigated the decline in outcrossed seed production by the abortion of selfed seeds when leaves were intensively damaged.
Enabling access: Reflecting on inclusive education practice
Authors: Kapcia Sophia 
Published: October 2024

AbstractInclusive education is a multifaceted approach that celebrates diversity, acknowledges individual differences and fosters an environment where all students can thrive. This reflective piece delves into key components of inclusive education practice, including embracing diversity, implementing differentiated instruction, fostering collaborative partnerships, creating an inclusive environment and engaging in continuous reflection and professional development. Drawing from over two decades of teaching experience, I seek to highlight the importance of catering to individual needs, building collaborative relationships and nurturing a culture of inclusivity and empowerment which are factors essential in creating inclusion and equity within the classroom. Through embracing inclusive practices, teachers can create learning communities where each student, regardless of their ability or challenges, has the opportunity to succeed.