bluuebunny/crossref_metadata_2025_split huggingface dataset:
ПОЛУЧЕНИЕ РЕКОМБИНАНТНОГО ПРОТЕИНА SPM2 THEILERIA ANNULATA
Authors: Рыскельдина А.Ж. , Абдикулов Б.Б. , Турсунбай Н.Е. , Шевцов А.Б. , Ержанова Н.С. , Куйбагаров М.А. 
Published: September 2024

Среди инфекций животных, передающихся клещами, по степени патогенности и ущерба, тейлериоз вызываемый Theileria annulata (T.annulata), является одним из наиболее злокачественных, с высоким процентом летальности. В Казахстане тейлериоз распространен в южных регионах. Высокий процент инфицированности, приводит к тому, что завозимый высокопродуктивный скот очень тяжело, с большим уровнем летальностью переносит заболевание. Среди поголовья мясного скота и овец данное заболевание приводит к резкому исхуданию животных, потере до 30% массы тела и ухудшению качества мясной продукции. Уровни удоев восстанавливаются до прежнего уровня в течение длительного срока (1-2 месяца) лишь до 80%. В рамках борьбы с тейлериозом крупного рогатого скота важным моментом является проведение систематических и достаточно масштабных серологических исследований восприимчивого поголовья, для чего необходимо наличие у ветеринарных специалистов высокочувствительных и специфичных диагностикумов.
Целью данной работы было получение рекомбинантного протеина T.annulata пригодного для использование в качестве антигена для серологической диагностики. В качестве целевого был выбран ген кодирующий протеин спорозоит и макрошизонт 2 (spm2) Theileria annulata (a sporozoite and macroschizont gene 2 (spm2)). Ген Spm2 T. annulata участвует в инвазии спорозоитов мононуклеарные клетки периферической крови крупного рогатого скота и экспрессируются на поверхности паразита на всех стадиях жизненного цикла. Была создана экспрессионная векторная система и отработан протокол очистки рекомбинантного белка Spm2, который предполагал металл-хелатную аффинную хроматографию с посаженными ионами Ni2+. SDS-PAGE анализ очищенных проб показал достаточно высокий выход чистых препаратов рекомбинантного белка Spm2 T. annulata с молекулярной массой 42 кДа. С целью проверки сероактивности полученного протеина, он был тестирован в качестве антигена в непрямом варианте твердофазного иммуноферментного анализа (ТИФА). В работе были использованы пробы плазмы крови КРС из хозяйств неблагополучного по тейлериозу региона Казахстана, в соответствующих образцах крови которых методом ПЦР, было определено наличие или отсутствие ДНК T.annulata. Сравнительный ИФА анализ 34 проб плазмы крови от ПЦР положительных животных с 10 пробами плазмы крови от ПЦР отрицательных животных из благополучного региона позволил определить отсекающее значение (cut off) и четко провести их дифференциацию. Корреляция результатов ИФА с данными ПЦР анализа, говорит о перспективности полученного антигена и необходимости дополнительных исследований по изучению его специфичности и возможности использования в качестве компонента ИФА тест-системы.
Geometric and Flow Characterization of Additively Manufactured Turbine Blades With Drilled Film Cooling Holes
Authors: McCormack Kelsey E. , Rozman Maria , Berdanier Reid A. , Thole Karen A. 
Published: September 2024

Abstract
As designers investigate new cooling technologies to advance future gas turbine engines, manufacturing methods that are fast and accurate are needed. Additive manufacturing facilitates the rapid prototyping of parts at a cost lower than conventional casting but is challenged in accurately reproducing small features such as turbulators, pin fins, and film cooling holes. This study explores the potential application of additive manufacturing and advanced hole drill methods as tools to investigate cooling technologies for future turbine blade designs. Data from computed tomography scans are used to nondestructively evaluate each of the cooling features in the blade. The resulting flow performance of these parts is further related to the manufacturing through benchtop flow testing. Results show that while total blade flow is consistent for all additively manufactured cooled blades, flow through smaller regions of the blades shows variations. Shaped film cooling holes manufactured using a high-speed electrical discharge machining method are within tolerance in the metering section but do not expand at the specified angle in the diffuser even though design tolerances are met. In contrast to high-speed EDM, conventional EDM holes are undersized throughout the length of the hole. Due to the additive manufacturing process, the surface roughness was higher on the additively manufactured parts in the current study than has been previously reported for surface roughness of commonly used cast components. The roughness results show high levels on thin walls, particularly at the trailing edge as well as on downskin surfaces. Internal surface roughness is higher than external roughness at most locations on the blade. The results of this study confirm that additive manufacturing along with advanced hole drilling techniques offers faster development of blade cooling designs.
Distinct negative-sense RNA viruses induce a common set of transcripts encoding proteins forming an extensive network
Authors: Hofmann Nina , Bartkuhn Marek , Becker Stephan , Biedenkopf Nadine , Böttcher-Friebertshäuser Eva , Brinkrolf Karina , Dietzel Erik , Fehling Sarah Katharina , Goesmann Alexander , Heindl Miriam Ruth , Hoffmann Simone , Karl Nadja , Maisner Andrea , Mostafa Ahmed , Kornecki Laura , Müller-Kräuter Helena , Müller-Ruttloff Christin , Nist Andrea , Pleschka Stephan , Sauerhering Lucie , Stiewe Thorsten , Strecker Thomas , Wilhelm Jochen , Wuerth Jennifer D. , Ziebuhr John , Weber Friedemann , Schmitz M. Lienhard 
Published: September 2024

ABSTRACT

The large group of negative-strand RNA viruses (NSVs) comprises many important pathogens. To identify conserved patterns in host responses, we systematically compared changes in the cellular RNA levels after infection of human hepatoma cells with nine different NSVs of different virulence degrees. RNA sequencing experiments indicated that the amount of viral RNA in host cells correlates with the number of differentially expressed host cell transcripts. Time-resolved differential gene expression analysis revealed a common set of 178 RNAs that are regulated by all NSVs analyzed. A newly developed open access web application allows downloads and visualizations of all gene expression comparisons for individual viruses over time or between several viruses. Most of the genes included in the core set of commonly differentially expressed genes (DEGs) encode proteins that serve as membrane receptors, signaling proteins and regulators of transcription. They mainly function in signal transduction and control immunity, metabolism, and cell survival. One hundred sixty-five of the DEGs encode host proteins from which 47 have already been linked to the regulation of viral infections in previous studies and 89 proteins form a complex interaction network that may function as a core hub to control NSV infections.

IMPORTANCE
The infection of cells with negative-strand RNA viruses leads to the differential expression of many host cell RNAs. The differential spectrum of virus-regulated RNAs reflects a large variety of events including anti-viral responses, cell remodeling, and cell damage. Here, these virus-specific differences and similarities in the regulated RNAs were measured in a highly standardized model. A newly developed app allows interested scientists a wide range of comparisons and visualizations.
Deep underground metagenome-assembled genomes from hydrothermal spring
Authors: Tarasov Kirill , Kravchenko Elena , Zarubin Mikhail , Yakhnenko Alena 
Published: September 2024

ABSTRACT

We report 19 metagenome-assembled genomes from a deep underground microbial community found in mineralized hydrothermal spring in the Baksan Neutrino Observatory tunnel. The community is predominantly occupied by members of Pseudomonadota (Gamma-, Beta-, and Alphaproteobacteria), Planctomycetota, Myxococcota, Nitrospirota, Cyanobacteria, Gemmatimonadota, and Armatimonadota.
Risk factors of skin tear in older persons: a protocol for systematic review and meta-analysis
Authors: Chen Lijuan , Zheng Nengtong , Jiang Hongzhan , Fan Siyue , Shen Jiali , Lin Huihui , Yang Liping , Yu Doudou 
Published: September 2024

IntroductionSkin tear (ST) will prolong the hospitalisation time of an older person, increase the cost of medical expenses and the difficulty in care for nursing staff, and seriously affect the quality of life of the older person. Early identification and intervention of the elderly at risk of ST are key factors in preventing the occurrence of ST in older persons. At present, risk factors for ST in older persons have not been systematically evaluated, let alone summarised to analyse risk factors for ST in older persons. Therefore, this systematic review and meta-analysis aims to synthesise existing research on risk factors for ST in older populations.Methods and analysisThe protocol is being reported by the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols statement. On 17 September 2023, we will start literature search in PubMed, Embase, Web of Science, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, Medline, Chinese Scientific Journal Database, Wan Fang Data Knowledge Service Platform, China National Knowledge Infrastructure, and Chinese Biomedical Literature Database. The language of the included literature is Chinese or English. Using RevMan V.5.4 software, we will perform a systematic review and meta-analysis of the final set of included studies to synthesise the data and draw meaningful conclusions. The Newcastle-Ottawa Quality Assessment Scale and the Agency for Healthcare Research and Quality will be used to assess the quality of the literature. TheI2test will be used to test heterogeneity.Ethics and disseminationEthical approval is not needed for this systematic review, as the study will not directly use information from human participants, and the data we use will be extracted from original studies. This systematic review and meta-analysis has been registered at the International Prospective Register of Systematic Reviews (PROSPERO). Once the systematic review and meta-analysis have been completed, we will publish our study in an academic journal.PROSPERO registration numberCRD42023460810.
Dementia mindset of caregivers providing residential care for older persons: study protocol for a replication study on the validation of the Dementia Mindset Scale
Authors: Albers Bernd , Bergmann Johannes Michael , Rodrigues Recchia Daniela , Müller-Widmer René , Hohmann Andreas , Pinkert Christiane , Berner Nora , Kunz Lena K , Teupen Sonja 
Published: September 2024

IntroductionProfessional caregivers’ perspectives on dementia and on people living with dementia (PlwD) can influence their feelings, judgements and behaviours in work situations, for example, how they think about symptoms, disease progression and the impact on a person’s quality of life. Their individual dementia mindset, which can be investigated with the 12-item Dementia Mindset Scale (DMS), might influence job satisfaction, work-related well-being and person-centred care. The aim of the proposed replication study is to confirm the results of the original study of the DMS and to test whether a malleable mindset is correlated with higher levels of caregiver education, dementia-specific professional experience/competence and dementia knowledge.Methods and analysisProfessional caregivers in residential care facilities for older persons who work directly with PlwD will be asked to answer an anonymous web-based online survey. The survey encompasses five standardised questionnaires: the DMS, the Dementia Knowledge Assessment Scale, the Oldenburg Burnout Inventory, the Job-related Affective Well-being Scale and the Sense of Competence in Dementia Care Staff Scale. In addition, job satisfaction, the educational background, professional experience and work situation are surveyed. For replication, the analyses will re-evaluate the psychometric properties (structural validity, model fit, internal consistency and predictive validity) by applying descriptive statistics, regression analysis, confirmatory factor analysis and correlation analysis. The additional analyses will use descriptive statistics, regression analysis and correlation analysis. Rasch analysis will be used to rank the difficulty of the items.Ethics and disseminationThis study was approved by the ethics committee of the German Society of Nursing Science (ID number 23-004). No personal information will be gathered. The results of the study will be distributed nationally and internationally through peer-reviewed academic journals, conferences, institutional websites and journals for nursing care practice.
Morphological changes in temporomandibular joint architecture in patients with temporomandibular disorders: systematic review protocol
Authors: Kadekuzhi Sarika , Karuveettil Vineetha , Prabha Rahul D , Vallikat Velath Ajith , N K Sapna Varma , Ghosh Parvathy , Suresh Sangeeth 
Published: September 2024

ObjectiveThe review involves the assessment of morphological variations in the temporomandibular joint (TMJ) and its associated structures in patients with temporomandibular disorder.IntroductionTemporomandibular disorders (TMD) are debilitating conditions that affect the TMJ complex, surrounding musculature and osseous components. Studies have reported that TMD result from morphological alterations in TMJ. These alterations can be efficiently studied using three-dimensional imaging. This review will summarise the morphological changes in TMJ and associated structures based on studies with three-dimensional imaging in patients with TMD.Inclusion criteriaThe systematic review will include studies with adult subjects with any one symptom of TMD and those studies that assessed TMJ morphology using three-dimensional imaging like CT, cone beam CT, MRI or arthrography.MethodsSystematic searches for relevant studies will be carried out in multiple databases. Sources will include MEDLINE, Scopus, Dentistry and Oral Sciences Source, Cochrane CENTRAL, CINAHL, Web of Science, ProQuest Dissertation and Thesis and Google Scholar. The databases will be searched from inception to November 2023. Analytical observational studies comprising retrospective and prospective cohort studies, case–control studies and analytical cross-sectional studies will be selected and critical appraisal will be performed. No restrictions will be imposed on the date and country of publication. Joanna Briggs Institute (JBI) guidelines for systematic effectiveness reviews will be followed for data appraisal, extraction and synthesis. The strength of evidence will be graded using the Grading of Recommendations, Assessment, Development and Evaluation method and the summary of findings will be created using GRADEpro software.Ethics and disseminationEthical approval is not applicable for this study since this involves analysis of secondary data. Results will be disseminated through peer-reviewed publications and cnference presentations. A comprehensive summary of morphological alterations in TMJ is essential for assessing risk factors, accurate diagnosis, treatment planning and will collectively contribute to enhanced clinical care and overall patient well-being.PROSPERO registration numberThe protocol is registered in PROSPERO: CRD42023448882.
Knowledge of and attitude towards depression among urban and rural residents in Beijing: a cross-sectional study
Authors: Cong Anan , Shang Lili , Yan Fang , Zhao Yanjie , Qi Han , Huang Qingzhi , Li Jingyuan , Sun Hui , Han Lili , Zhang Feifei , Li Shuxuan , Ma Laifu , Tian Qinghua , Zhou Qi , Zhang Ling , Wang Gang 
Published: September 2024

ObjectivesTo compare the level of knowledge of depression, recognition ability and attitudes towards depression among urban and rural residents in Beijing.DesignA cross-sectional study.SettingSix districts in Beijing, China, 2021.ParticipantsA total of 6463 participants aged 18 years and above who had lived for more than 6 months over the last year in Beijing were selected in this study.OutcomeThe awareness and recognition of depression and the views of residents towards people with depression.ResultsA total of 2554 urban and 2043 rural residents completed the survey. Urban residents of Beijing exhibited a higher average total score on the Depression Knowledge Questionnaire [(20.4±3.3) vs (18.7±3.5), p<0.001] and a higher rate of correctly identifying individuals with depression (47.9% vs 36.6%, p<0.001) than their counterparts in rural areas. Residents who correctly identified people with depression had higher scores on the Depression Knowledge Questionnaire. Depression knowledge varied significantly among urban and rural residents. The multivariate linear regression analysis revealed that rural residents scored significantly lower on measures of depression knowledge compared with urban residents (B=−0.83, 95%CI=−1.03 to −0.63, p<0.001). Older individuals (aged 50+) showed lower understanding compared with the 18–49 age group, with significant negative regression coefficients (Urban:B=−1.06, Rural:B=−1.35, both p<0.001). Higher educational levels were positively associated with greater depression knowledge (Urban:B=1.40, Rural:B=1.21, both p<0.001). Employment was linked to higher knowledge levels than unemployment (Urban:B=−0.60, Rural:B=−0.58, both p=0.00). A monthly income of 8000 yuan or more correlated with better depression understanding than lower incomes (Urban:B=0.81, Rural:B=1.04, both p<0.001). Additionally, in urban areas, unmarried residents scored higher in depression knowledge than those divorced (B=−0.55, p=0.04). Residents in urban areas had relatively positive attitudes towards individuals with depression.ConclusionsRural residents of Beijing had lower levels of knowledge and recognition of depression and more negative attitudes towards individuals with depression than those from urban areas. The health authority needs to focus on the poor level of knowledge and increase mental health resources in rural areas as a priority site for future psychological popularisation efforts.
Association between ambient temperature and economic burden of unintentional injury in Tianjin: a case-crossover study
Authors: Li Yue , Yuan Chao , Liu Tao , Yang Zhao , Li Fangguo , Li Ji , Fan Haojun , Cao Chunxia 
Published: September 2024

ObjectiveUnintentional injuries constitute a significant global public health issue with significant social and economic costs. Previous evidence suggests ambient temperatures are associated with unintentional injury occurrences. However, the impacts of ambient temperature on unintentional injury economic burden have received little research attention. The objective of the study was to examine the association between ambient temperature and economic burden of unintentional injury.DesignTime-stratified case-crossover study.SettingThis study was performed at Tianjin Hospital, the largest trauma centre in Tianjin, by applying a hospital-based time-stratified case-crossover study.ParticipantsThe 12 241 patients admitted with unintentional injuries and meteorological data were collected in Tianjin, China in 2021.Primary and secondary outcomeThe association between ambient temperature and unintentional injury hospitalisation was evaluated with a distributed lag non-linear model, further temperature-attributable economic burden of unintentional injuries was quantified, and adjusted for demographic characteristics, injury mechanism and injury location of injury.ResultsThe temperatures below 11.5°C were significantly associated with the increased risk of unintentional injury hospitalisation in Tianjin, in 2021. The effect was maximised on the current day. The relatively low temperature was responsible for 25.44% (95% CI 13.74, 33.09) of unintentional injury patients, and was associated with the number of unintentional injury patients (3114, 95% CI 1608, 4036). The relatively low temperature was associated with the excess economic burden for unintentional injury (¥197.52 million, 95% CI 102.00, 256.00; about 27.10 million dollars), accounting for 26.49% of the total economic burden. The cold temperatures generally had greater impacts on males (¥136.46 million, 95% CI 83.28, 172.42; about 18.67 million dollars) and the elderly (¥74.35 million, 95% CI 14.87, 102.14; about 10.24 million dollars).ConclusionThe temperature was associated with approximately 3000 unintentional injury patients and ¥200 million (27 million dollars), accounting for 26% of the total economic burden in Tianjin, 2021.
Investigating the relationship between spermogram parameters and sexual desire in infertile men: a cross-sectional study from a tertiary centre in Iran
Authors: Amirjannati Naser , Akhavizadegan Hamed , Mohazzab Arash , Ghorbani Behzad , Lotfi Razieh , Fathalian Manizheh 
Published: September 2024

BackgroundMale factor infertility can affect spermatogenesis, sexual desire, and thus the quality of life of couples. The present study was conducted to investigate the relationship between spermogram parameters, and the score of sexual desire in infertile men.MethodsThis cross-sectional study was conducted on 315 infertile men referred to the Avicenna Infertility Center of Tehran (March 2022 to March 2023). The participants were selected based on the results of previous spermogram and hormonal tests recorded in their medical records. Eligible men completed the demographic information questionnaire and Hurlbert Index of Sexual Desire. A multivariable linear regression model was used to adjust the effect of variables on Hurlbert’s score.ResultsThere was no significant relationship among sperm parameters (count, morphology, motility, vitality, concentration and DNA Fragmentation Index) and with sexual desire of infertile men. Education level, age of men and their partners, the duration of the marriage and duration of infertility did not have a statistically significant effect on sexual desire. However, economic status had an inverse effect on men’s sexual desire, with regression coefficients of 7.37 and 7.78 for medium and low socioeconomic levels compared with high (p<0.05).ConclusionMale sexual desire is primarily influenced by social factors rather than organic ones. Further multicentre prospective studies are recommended for more accurate results.