bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rates of Hospitalization for Dehydration Following Hurricane Sandy in New Jersey
Authors: Swerdel Joel N. , Rhoads George G. , Cosgrove Nora M. , Kostis John B. ,   for the Myocardial Infarction Data Acquisition System (MIDAS 25) Study Group
Published: December 2015

AbstractObjectiveHurricane Sandy, one of the most destructive natural disasters in New Jersey history, made landfall on October 29, 2012. Prolonged loss of electrical power and extensive infrastructure damage restricted access for many to food and water. We examined the rate of dehydration in New Jersey residents after Hurricane Sandy.MethodsWe obtained data from 2008 to 2012 from the Myocardial Infarction Data Acquisition System (MIDAS), a repository of in-patient records from nonfederal New Jersey hospitals (N=517,355). Patients with dehydration had ICD-9-CM discharge diagnosis codes for dehydration, volume depletion, and/or hypovolemia. We used log-linear modeling to estimate the change in in-patient hospitalizations for dehydration comparing 2 weeks after Sandy with the same period in the previous 4 years (2008–2011).ResultsIn-patient hospitalizations for dehydration were 66% higher after Sandy than in 2008–2011 (rate ratio [RR]: 1.66; 95% confidence interval [CI]: 1.50, 1.84). Hospitalizations for dehydration in patients over 65 years of age increased by nearly 80% after Sandy compared with 2008–2011 (RR: 1.79; 95% CI: 1.58, 2.02).ConclusionSandy was associated with a marked increase in hospitalizations for dehydration. Reducing the rate of dehydration following extreme weather events is an important public health concern that needs to be addressed, especially in those over 65 years of age. (Disaster Med Public Health Preparedness. 2016;10:188–192)
Making Sense of the ‘Chemical Revolution’. Patients’ Voices on the Introduction of Neuroleptics in the 1950s
Authors: Majerus Benoît 
Published: December 2015

The so-called chemical revolution has produced a vast historiographical corpus. Yet the patient’s voice remains surprisingly absent from these stories. Based on the archives of theInstitut de Psychiatrie (Brussels), this paper traces the introduction of Largactil as recounted in patient letters, physician records and nurse notes. The paper thus contributes to the history of therapies from below, but also participates in the historiographical debate about whether the introduction of neuroleptics can indeed be considered a revolution.
Archaeological retrospect I
Authors: Phillips Charles 
Published: December 2015

Looking back over a concern with archaeology which has lasted for half a century I am grateful to have been present through a crucial period in its development.
There were few advantages in my earliest days. On my father’s side I come from a line of farmers, land appraisers, bailiffs and the like which I can trace back in some detail for nearly three hundred years, living for the most part in Northwest Essex; on my mother’s side my ancestors were North Wessex peasants and inn-keepers, with a probable admixture of Welsh cattle-drover blood at the end of the eighteenth century. Wantage and Abingdon were their local centres. All with the exception of one or two backsliders were strict Evangelicals, often wavering on the verge of Dissent, and none ever aspired to any higher standard of education than one who entered the Congregational ministry in 1827. Any known interest in learning or antiquity showed itself more among the Wessex peasants than the clod-hoppers of Essex. My maternal grandfather, who was a native of the Vale of White Horse, was personally known to me and was interested in the many relics of the past observable about him, an intelligent man but with little formal instruction.
Testing a back-arc ‘aulacogen’ model for the Central Metasedimentary Belt of the Grenville Province
Authors: DICKIN ALAN , HYNES EDEN , STRONG JACOB , WISBORG MARK 
Published: December 2015

AbstractNearly 70 new Nd isotope analyses are presented for plutonic orthogneisses from the Grenvillian Central Metasedimentary Belt (CMB) in order to test a back-arc aulacogen model for its origin. Nd isotope signatures of metaplutonic rocks are used as probes of the formation age of the crust at depth, revealing sharp boundaries between old crustal blocks and juvenile (1.2–1.35 Ga) Elzevirian-age crust. Firstly, a hidden block of old crustal basement is revealed between areas of juvenile crust south of Douglas, Ontario. Secondly, TDM ages refine the boundary between juvenile crust and old basement (1.35–1.55 Ga) within the Weslemkoon batholith, showing this pluton to be a polygenetic stitching pluton that straddles a hidden crustal boundary. Finally, the CMB boundary zone is shown to form a sharp age boundary between juvenile and old crustal domains, and is interpreted as a reactivated rift-bounding normal fault. When the distribution of rift-related alkaline rocks is compared with these crustal boundaries, the Bancroft nepheline syenite suite is centrally located in a juvenile ensimatic zone between blocks of old basement. Such a location, near the axis of a juvenile crustal segment, implies emplacement late in the rifting process. Similarly, the Blue Mountain nepheline syenite appears to post-date an earlier rifting event to the southeast. Hence, a multi-stage model is proposed for the evolution of a back-arc aulacogen, which is consistent with the distribution of marble and volcanic/plutonic units in the CMB. The model places the Bancroft nepheline syenites in a precise plate tectonic context for the first time.
Multi-symplectic magnetohydrodynamics: II, addendum and erratum
Authors: Webb G. M. , McKenzie J. F. , Zank G. P. 
Published: December 2015

A recent paper by Webb et al. (J. Plasma Phys., vol. 80, 2014, pp. 707–743) on multi-symplectic magnetohydrodynamics (MHD) using Clebsch variables in an Eulerian action principle with constraints is further extended. We relate a class of symplecticity conservation laws to a vorticity conservation law, and provide a corrected form of the Cartan–Poincaré differential form formulation of the system. We also correct some typographical errors (omissions) in Webb et al. (J. Plasma Phys., vol. 80, 2014, pp. 707–743). We show that the vorticity–symplecticity conservation law, that arises as a compatibility condition on the system, expressed in terms of the Clebsch variables is equivalent to taking the curl of the conservation form of the MHD momentum equation. We use the Cartan–Poincaré form to obtain a class of differential forms that represent the system using Cartan’s geometric theory of partial differential equations
Life History and Risk of Death after 50: A Survival Analysis for Europe
Authors: Nicińska Anna , Kalbarczyk-Stęclik Małgorzata 
Published: December 2015

RÉSUMÉDans notre étude nous avons examiné l’influence des événements du passé des individus sur le risque de décès des Européens âgés de plus de 50 ans, en contrôlant autres variables pertinentes. Notre analyse était basée sur les données d’entretiens biographiques rétrospectifs, les données d’entretiens réguliers au suivi longitudinal et celles d’entretiens de fin de vie de l’Enquête européenne SHARE sur la santé, le vieillissement et la retraite en Europe. En particulier, nous relevons l’état de santé auto-déclaré pendant l’enfance; les périodes de pauvreté, de faim et de mauvaise santé éprouvées dans le passé; et aussi l’histoire des soins de santé, y compris les soins dentaires, les analyses de sang et les mesures de pression artérielle. Ces informations, avec l’age, le sexe, l’état de santé subjectif et objectif, et d’autres facteurs socio-démographiques, permettent d’expliquer le risque de décès. L’analyse de survie, en contrôlant des variables pertinentes, montre que les événements du passé ont un impact significatif sur le risque de décès des Européens âgés de plus de 50 ans.
Comparative efficacy of four candidate DNA barcode regions for identification of Vicia species
Authors: Raveendar Sebastin , Lee Jung-Ro , Shim Donghwan , Lee Gi-An , Jeon Young-Ah , Cho Gyu-Taek , Ma Kyung-Ho , Lee Sok-Young , Sung Gi-Ho , Chung Jong-Wook 
Published: December 2015

AbstractThe genus Vicia L., one of the earliest domesticated plant genera, is a member of the legume tribe Fabeae of the subfamily Papilionoideae (Fabaceae). The taxonomic history of this genus is extensive and controversial, which has hindered the development of taxonomic procedures and made it difficult to identify and share these economically important crop resources. Species identification through DNA barcoding is a valuable taxonomic classification tool. In this study, four DNA barcodes (ITS2, matK, rbcL and psbA-trnH) were evaluated on 110 samples that represented 34 taxonomically best-known species in the Vicia genus. Topologies of the phylogenetic trees based on an individual locus were similar. Individual locus-based analyses could not discriminate closely related Vicia species. We proposed a concatenated data approach to increase the resolving power of ITS2. The DNA barcodes matK, psbA-trnH and rbcL were used as an additional tool for phylogenetic analysis. Among the four barcodes, three-barcode combinations that included psbA-trnH with any two of the other barcodes (ITS2, matK or rbcL) provided the best discrimination among Vicia species. Species discrimination was assessed with bootstrap values and considered successful only when all the conspecific individuals formed a single clade. Through sequencing of these barcodes from additional Vicia accessions, 17 of the 34 known Vicia species could be identified with varying levels of confidence. From our analyses, the combined barcoding markers are useful in the early diagnosis of targeted Vicia species and can provide essential baseline data for conservation strategies, as well as guidance in assembling germplasm collections.
Acceptorless dehydrogenation of small molecules through cooperative base metal catalysis
Authors: West Julian G. , Huang David , Sorensen Erik J. 
Published: December 2015

AbstractThe dehydrogenation of unactivated alkanes is an important transformation both in industrial and biological systems. Recent efforts towards this reaction have revolved around high temperature, organometallic C–H activation by noble metal catalysts that produce alkenes and hydrogen gas as the sole products. Conversely, natural desaturase systems proceed through stepwise hydrogen atom transfer at physiological temperature; however, these transformations require a terminal oxidant. Here we show combining tetra-n-butylammonium decatungstate (TBADT) and cobaloxime pyridine chloride (COPC) can catalytically dehydrogenate unactivated alkanes and alcohols under near-UV irradiation at room temperature with hydrogen as the sole by-product. This noble metal-free process follows a nature-inspired pathway of high- and low-energy hydrogen atom abstractions. The hydrogen evolution ability of cobaloximes is leveraged to render the system catalytic, with cooperative turnover numbers up to 48 and yields up to 83%. Our results demonstrate how cooperative base metal catalysis can achieve transformations previously restricted to precious metal catalysts.
Interneuronal DISC1 regulates NRG1-ErbB4 signalling and excitatory–inhibitory synapse formation in the mature cortex
Authors: Seshadri Saurav , Faust Travis , Ishizuka Koko , Delevich Kristen , Chung Youjin , Kim Sun-Hong , Cowles Martis , Niwa Minae , Jaaro-Peled Hanna , Tomoda Toshifumi , Lai Cary , Anton E. S. , Li Bo , Sawa Akira 
Published: December 2015

AbstractNeuregulin-1 (NRG1) and its receptor ErbB4 influence several processes of neurodevelopment, but the mechanisms regulating this signalling in the mature brain are not well known. DISC1 is a multifunctional scaffold protein that mediates many cellular processes. Here we present a functional relationship between DISC1 and NRG1-ErbB4 signalling in mature cortical interneurons. By cell type-specific gene modulation in vitro and in vivo including in a mutant DISC1 mouse model, we demonstrate that DISC1 inhibits NRG1-induced ErbB4 activation and signalling. This effect is likely mediated by competitive inhibition of binding of ErbB4 to PSD95. Finally, we show that interneuronal DISC1 affects NRG1-ErbB4-mediated phenotypes in the fast spiking interneuron-pyramidal neuron circuit. Post-mortem brain analyses and some genetic studies have reported interneuronal deficits and involvement of the DISC1, NRG1 and ErbB4 genes in schizophrenia, respectively. Our results suggest a mechanism by which cross-talk between DISC1 and NRG1-ErbB4 signalling may contribute to these deficits.
A new tool called DISSECT for analysing large genomic data sets using a Big Data approach
Authors: Canela-Xandri Oriol , Law Andy , Gray Alan , Woolliams John A. , Tenesa Albert 
Published: December 2015

AbstractLarge-scale genetic and genomic data are increasingly available and the major bottleneck in their analysis is a lack of sufficiently scalable computational tools. To address this problem in the context of complex traits analysis, we present DISSECT. DISSECT is a new and freely available software that is able to exploit the distributed-memory parallel computational architectures of compute clusters, to perform a wide range of genomic and epidemiologic analyses, which currently can only be carried out on reduced sample sizes or under restricted conditions. We demonstrate the usefulness of our new tool by addressing the challenge of predicting phenotypes from genotype data in human populations using mixed-linear model analysis. We analyse simulated traits from 470,000 individuals genotyped for 590,004 SNPs in ∼4 h using the combined computational power of 8,400 processor cores. We find that prediction accuracies in excess of 80% of the theoretical maximum could be achieved with large sample sizes.
Systematic investigation and microbial community profile of indole degradation processes in two aerobic activated sludge systems
Authors: Ma Qiao , Qu Yuanyuan , Zhang Xuwang , Liu Ziyan , Li Huijie , Zhang Zhaojing , Wang Jingwei , Shen Wenli , Zhou Jiti 
Published: December 2015

AbstractIndole is widely spread in various environmental matrices. Indole degradation by bacteria has been reported previously, whereas its degradation processes driven by aerobic microbial community were as-yet unexplored. Herein, eight sequencing batch bioreactors fed with municipal and coking activated sludges were constructed for aerobic treatment of indole. The whole operation processes contained three stages, i.e. stage I, glucose and indole as carbon sources; stage II, indole as carbon source; and stage III, indole as carbon and nitrogen source. Indole could be completely removed in both systems. Illumina sequencing revealed that alpha diversity was reduced after indole treatment and microbial communities were significantly distinct among the three stages. At genus level, Azorcus and Thauera were dominant species in stage I in both systems, while Alcaligenes, Comamonas and Pseudomonas were the core genera in stage II and III in municipal sludge system, Alcaligenes and Burkholderia in coking sludge system. In addition, four strains belonged to genera Comamonas, Burkholderia and Xenophilus were isolated using indole as sole carbon source. Burkholderia sp. IDO3 could remove 100 mg/L indole completely within 14 h, the highest degradation rate to date. These findings provide novel information and enrich our understanding of indole aerobic degradation processes.
The ratio of FoxA1 to FoxA2 in lung adenocarcinoma is regulated by LncRNA HOTAIR and chromatin remodeling factor LSH
Authors: Wang Ranran , Shi Ying , Chen Ling , Jiang Yiqun , Mao Chao , Yan Bin , Liu Shuang , Shan Bin , Tao Yongguang , Wang Xiang 
Published: December 2015

AbstractThe lncRNA HOTAIR is a critical regulator of cancer progression. Chromatin remodeling factor LSH is critical for normal development of plants and mammals. However, the underlying mechanisms causing this in cancer are not entirely clear. The functional diversification of the FOXA1 and FOXA2 contributes to the target genes during evolution and carcinogenesis. Little is known about the ratio of FOXA1 to FOXA2 in cancer. We here found that both HOTAIR and LSH overexpression was significantly correlated with poor survival in patients with lung adenocarcinoma cancer (ADC). Also, the ratio of FOXA1 and FOXA2 is linked with poor survival in patients with lung ADC. HOTAIR regulates the ratio of FOXA1 to FOXA2 and migration and invasion. HOTAIR and the ratio of FOXA1 to FOXA2 are negatively correlated. HOTAIR knockdown inhibits migration and invasion. HOTAIR is associated with LSH and this association linked with the binding of LSH in the promoter of FOXA1, not FOXA2. Targeted inhibition of HOTAIR suppresses the migratory and invasive properties. These data suggest that HOTAIR is an important mediator of the ratio of FOXA1 and FOXA2 and LSH involves in and suggest that HOTAIR inhibition may represent a promising therapeutic option for suppressing lung ADC progression.
A novel, native-format bispecific antibody triggering T-cell killing of B-cells is robustly active in mouse tumor models and cynomolgus monkeys
Authors: Smith Eric J. , Olson Kara , Haber Lauric J. , Varghese Bindu , Duramad Paurene , Tustian Andrew D. , Oyejide Adelekan , Kirshner Jessica R. , Canova Lauren , Menon Jayanthi , Principio Jennifer , MacDonald Douglas , Kantrowitz Joel , Papadopoulos Nicholas , Stahl Neil , Yancopoulos George D. , Thurston Gavin , Davis Samuel 
Published: December 2015

AbstractBispecific antibodies, while showing great therapeutic potential, pose formidable challenges with respect to their assembly, stability, immunogenicity and pharmacodynamics. Here we describe a novel class of bispecific antibodies with native human immunoglobulin format. The design exploits differences in the affinities of the immunoglobulin isotypes for Protein A, allowing efficient large-scale purification. Using this format, we generated a bispecific antibody, REGN1979, targeting the B cell marker, CD20 and the CD3 component of the T cell receptor, which triggers redirected killing of B cells. In mice, this antibody prevented growth of B cell tumors and also caused regression of large established tumors. In cynomolgus monkeys, low doses of REGN1979 caused prolonged depletion of B cells in peripheral blood with a serum half-life of approximately 14 days. Further, the antibody induced a deeper depletion of B cells in lymphoid organs than rituximab. This format has broad applicability for development of clinical bispecific antibodies.
Uncovering MicroRNA Regulatory Hubs that Modulate Plasma Cell Differentiation
Authors: Tsai Dong-Yan , Hung Kuo-Hsuan , Lin I-Ying , Su Shin-Tang , Wu Shih-Ying , Chung Cheng-Han , Wang Tong-Cheng , Li Wen-Hsiung , Shih Arthur Chun-Chieh , Lin Kuo-I 
Published: December 2015

AbstractUsing genome-wide approaches, we studied the microRNA (miRNA) expression profile during human plasma cell (PC) differentiation induced by stimulation of human blood B cells with T follicular helper cell–dependent signals. Combining the profiles of differentially expressed genes in PC differentiation with gene ontology (GO) analysis revealed that a significant group of genes involved in the transcription factor (TF) activity was preferentially changed. We thus focused on studying the effects of differentially expressed miRNAs on several key TFs in PC differentiation. Cohorts of differentially expressed miRNAs cooperating as miRNA hubs were predicted and validated to modulate key TFs, including a down-regulated miRNA hub containing miR-101-3p, -125b-5p and -223-3p contributing to induction of PRDM1 as well as an up-regulated miRNA hub containing miR-34a-5p, -148a-3p and -183-5p suppressing BCL6, BACH2 and FOXP1. Induced expression of NF-κB and PRDM1 during PC differentiation controlled the expression of up- and down-regulated miRNA hubs, respectively. Co-expression of miR-101-3p, -125b-5p and -223-3p in stimulated B cells showed synergistic effects on inhibition of PC formation, which can be rescued by re-introduction of PRDM1. Together, we catalogue the complex roadmap of miRNAs and their functional interplay in collaboratively directing PC differentiation.
Microspectrometric insights on the uptake of antibiotics at the single bacterial cell level
Authors: Cinquin Bertrand , Maigre Laure , Pinet Elizabeth , Chevalier Jacqueline , Stavenger Robert A. , Mills Scott , Réfrégiers Matthieu , Pagès Jean-Marie 
Published: December 2015

AbstractBacterial multidrug resistance is a significant health issue. A key challenge, particularly in Gram-negative antibacterial research, is to better understand membrane permeation of antibiotics in clinically relevant bacterial pathogens. Passing through the membrane barrier to reach the required concentration inside the bacterium is a pivotal step for most antibacterials. Spectrometric methodology has been developed to detect drugs inside bacteria and recent studies have focused on bacterial cell imaging. Ultimately, we seek to use this method to identify pharmacophoric groups which improve penetration and therefore accumulation, of small-molecule antibiotics inside bacteria. We developed a method to quantify the time scale of antibiotic accumulation in living bacterial cells. Tunable ultraviolet excitation provided by DISCO beamline (synchrotron Soleil) combined with microscopy allows spectroscopic analysis of the antibiotic signal in individual bacterial cells. Robust controls and measurement of the crosstalk between fluorescence channels can provide real time quantification of drug. This technique represents a new method to assay drug translocation inside the cell and therefore incorporate rational drug design to impact antibiotic uptake.
The transcription factor ccaat/enhancer binding protein β (C/EBPβ) and miR-27a regulate the expression of porcine Dickkopf2 (DKK2)
Authors: Tao Hu , Wang Lei , Zhou Jiawei , Pang Panfei , Cai Shanzhi , Li Jialian , Mei Shuqi , Li Fenge 
Published: December 2015

AbstractUsing Affymetrix porcine Gene-Chip analyses, we found that Dickkopf2 (DKK2), a WNT antagonist, is differentially expressed in pre-ovulatory follicles between Large White and Chinese Taihu sows. This study aims to identify the regulatory factors responsible for DKK2 expression. Deletion fragment and mutation analyses identified DKK2-D3 as the porcine DKK2 core promoter. There were four C/EBPβ binding sites within the DKK2 core promoter. The C allele that results from a spontaneous alteration (DKK2 c.−1130 T > C) in the core promoter was associated with a higher total number born (TNB) and a higher number born alive (NBA) in all parities in a synthetic pig population. This was possibly the result of a change in C/EBPβ binding ability, which was confirmed using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays (EMSA). Moreover, C/EBPβ specifically bound to and activated the DKK2 promoter, as revealed by mutation analysis, overexpression and RNA interference (RNAi) experiments. We also confirmed that miR-27a is a negative regulator of the DKK2 gene using miR-27a overexpression and inhibition experiments and mutation analyses. RTCA xCELLigence experiments showed that miR-27a suppressed Chinese hamster ovary (CHO) cell proliferation by down-regulating DKK2 gene expression. Taken together, our findings suggest that C/EBPβ and miR-27a control DKK2 transcription.