bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic and Prognostic Implications of BRAF V600E in Pediatric Low-Grade Gliomas
Authors: Lassaletta Alvaro , Zapotocky Michal , Mistry Matthew , Ramaswamy Vijay , Honnorat Marion , Krishnatry Rahul , Guerreiro Stucklin Ana , Zhukova Nataliya , Arnoldo Anthony , Ryall Scott , Ling Catriona , McKeown Tara , Loukides Jim , Cruz Ofelia , de Torres Carmen , Ho Cheng-Ying , Packer Roger J. , Tatevossian Ruth , Qaddoumi Ibrahim , Harreld Julie H. , Dalton James D. , Mulcahy-Levy Jean , Foreman Nicholas , Karajannis Matthias A. , Wang Shiyang , Snuderl Matija , Nageswara Rao Amulya , Giannini Caterina , Kieran Mark , Ligon Keith L. , Garre Maria Luisa , Nozza Paolo , Mascelli Samantha , Raso Alessandro , Mueller Sabine , Nicolaides Theodore , Silva Karen , Perbet Romain , Vasiljevic Alexandre , Faure Conter Cécile , Frappaz Didier , Leary Sarah , Crane Courtney , Chan Aden , Ng Ho-Keung , Shi Zhi-Feng , Mao Ying , Finch Elizabeth , Eisenstat David , Wilson Bev , Carret Anne Sophie , Hauser Peter , Sumerauer David , Krskova Lenka , Larouche Valerie , Fleming Adam , Zelcer Shayna , Jabado Nada , Rutka James T. , Dirks Peter , Taylor Michael D. , Chen Shiyi , Bartels Ute , Huang Annie , Ellison David W. , Bouffet Eric , Hawkins Cynthia , Tabori Uri 
Published: July 2017

Purpose BRAF V600E is a potentially highly targetable mutation detected in a subset of pediatric low-grade gliomas (PLGGs). Its biologic and clinical effect within this diverse group of tumors remains unknown. Patients and Methods A combined clinical and genetic institutional study of patients with PLGGs with long-term follow-up was performed (N = 510). Clinical and treatment data of patients with BRAF V600E mutated PLGG (n = 99) were compared with a large international independent cohort of patients with BRAF V600E mutated-PLGG (n = 180). Results BRAF V600E mutation was detected in 69 of 405 patients (17%) with PLGG across a broad spectrum of histologies and sites, including midline locations, which are not often routinely biopsied in clinical practice. Patients with BRAF V600E PLGG exhibited poor outcomes after chemotherapy and radiation therapies that resulted in a 10-year progression-free survival of 27% (95% CI, 12.1% to 41.9%) and 60.2% (95% CI, 53.3% to 67.1%) for BRAF V600E and wild-type PLGG, respectively ( P < .001). Additional multivariable clinical and molecular stratification revealed that the extent of resection and CDKN2A deletion contributed independently to poor outcome in BRAF V600E PLGG. A similar independent role for CDKN2A and resection on outcome were observed in the independent cohort. Quantitative imaging analysis revealed progressive disease and a lack of response to conventional chemotherapy in most patients with BRAF V600E PLGG. Conclusion BRAF V600E PLGG constitutes a distinct entity with poor prognosis when treated with current adjuvant therapy.
Lysosomal targeting of SIDT2 via multiple YxxΦ motifs is required for SIDT2 function in the process of RNautophagy
Authors: Contu Viorica Raluca , Hase Katsunori , Kozuka-Hata Hiroko , Oyama Masaaki , Fujiwara Yuuki , Kabuta Chihana , Takahashi Masayuki , Hakuno Fumihiko , Takahashi Shin-Ichiro , Wada Keiji , Kabuta Tomohiro 
Published: July 2017

ABSTRACT
RNA degradation is an essential process for maintaining cellular homeostasis. Previously, we discovered a novel RNA degradation system, RNautophagy, during which direct import of RNA into lysosomes in an ATP-dependent manner followed by degradation takes place. The putative nucleic acid transporter SID-1 transmembrane family member 2 (SIDT2) predominantly localizes to lysosomes and mediates the translocation of RNA into lysosomes during RNautophagy. However, little is known about the mechanisms of sorting SIDT2 to lysosomes. Here, we show that three cytosolic YxxΦ motifs (in which x is any amino acid and Φ is an amino acid with a bulky hydrophobic side chain) are required for the lysosomal localization of SIDT2, and that SIDT2 interacts with adaptor protein complexes AP-1 and AP-2. We also find that localization to lysosomes by these three motifs is necessary for SIDT2 function in the process of RNautophagy, and that SIDT2 strikingly increases endogenous RNA degradation at the cellular level. To our knowledge, this is the first study to report an endogenous intracellular protein for which overexpression substantially increased intracellular RNA degradation. This study provides new insight into lysosomal targeting of proteins and intracellular RNA degradation, and further confirms the critical function of SIDT2 in RNautophagy.
This article has an associated First Person interview with the first author of the paper.
Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues
Authors: Demy Doris Lou , Tauzin Muriel , Lancino Mylène , Le Cabec Véronique , Redd Michael , Murayama Emi , Maridonneau-Parini Isabelle , Trede Nikolaus , Herbomel Philippe 
Published: July 2017

ABSTRACT
Macrophages infiltrate and establish in developing organs from an early stage, often before these have become vascularized. Similarly, leukocytes, in general, can quickly migrate through tissues to any site of wounding. This unique capacity is rooted in their characteristic amoeboid motility, the genetic basis of which is poorly understood. Trim33 (also known as Tif1-γ), a nuclear protein that associates with specific DNA-binding transcription factors to modulate gene expression, has been found to be mainly involved in hematopoiesis and gene regulation mediated by TGF-β. Here, we have discovered that in Trim33-deficient zebrafish embryos, primitive macrophages are unable to colonize the central nervous system to become microglia. Moreover, both macrophages and neutrophils of Trim33-deficient embryos display a reduced basal mobility within interstitial tissues, and a profound lack of a response to inflammatory recruitment signals, including local bacterial infections. Correlatively, Trim33-deficient mouse bone marrow-derived macrophages display a strongly reduced three-dimensional amoeboid mobility in fibrous collagen gels. The transcriptional regulator Trim33 is thus revealed as being essential for the navigation of macrophages and neutrophils towards developmental or inflammatory cues within vertebrate tissues.
Molecular anatomy of the early events in STIM1 activation – oligomerization or conformational change?
Authors: Korzeniowski Marek K. , Wisniewski Eva , Baird Barbara , Holowka David A. , Balla Tamas 
Published: July 2017

ABSTRACT
Decreased luminal endoplasmic reticulum (ER) Ca2+ concentration triggers oligomerization and clustering of the ER Ca2+ sensor STIM1 to promote its association with plasma membrane Orai1 Ca2+ channels leading to increased Ca2+ influx. A key step in STIM1 activation is the release of its SOAR domain from an intramolecular clamp formed with the STIM1 first coiled-coil (CC1) region. Using a truncated STIM1(1–343) molecule that captures or releases the isolated SOAR domain depending on luminal ER Ca2+ concentrations, we analyzed the early molecular events that control the intramolecular clamp formed between the CC1 and SOAR domains. We found that STIM1 forms constitutive dimers, and its CC1 domain can bind the SOAR domain of another STIM1 molecule in trans. Artificial oligomerization failed to liberate the SOAR domain or activate STIM1 unless the luminal Ca2+-sensing domains were removed. We propose that the release of SOAR from its CC1 interaction is controlled by changes in the orientation of the two CC1 domains in STIM1 dimers. Ca2+ unbinding in the STIM1 luminal domains initiates the conformational change allowing SOAR domain liberation and clustering, leading to Orai1 channel activation.
‘Moderne Muziek’: Vinyl magazine and the Dutch post-punk movement
Authors: Foster Richard 
Published: July 2017

Abstract
The Dutch music magazine Vinyl was launched at a chaotic party on 14 February 1981, at the newly opened ‘Schafthuis Royaal’ club in the squatted former-NRC building in the centre of Amsterdam. Vinyl presented itself as the champion of a new musical development unfolding in the Netherlands – ‘Ultra’. Ultra, standing for ultramodernen, was a term that described a number of young Dutch bands who made avant-garde, post-punk music, most notably Minny Pops, Mecano, Plus Instruments, Minioon, Mekanik Kommando, The Young Lions and Tox Modell. Ultra was visible mainly in Amsterdam, but also had a strong presence in cities such as Nijmegen, Eindhoven and ‘s-Hertogenbosch. From that February 1981 issue (preceded by a taster incorporated into the first edition, known as the ‘Zero Issue’ of December 1980), Vinyl rapidly expanded, reaching print runs of 15,000, including a print run of 3000 for an English language edition during its second year. At its peak in the mid-1980s, albeit with a more populist editorial policy, Vinyl was an established presence in the European alternative music press and referenced by the likes of BBC Radio 1 deejay John Peel. The magazine ran until February 1988. Taking a selected number of early editions of the magazine, this article looks to show how Vinyl carved out a distinct post-punk identity for itself and its readers in the Netherlands of the early 1980s. And, by using comparisons with contemporary articles on the Dutch post-punk scene in the British music press, the article will show that Vinyl (often driven by unaccommodating domestic circumstances) looked to promote an apolitical, transnational post-punk agenda.
Local government reform: Community planning and the quality of life in Northern Ireland
Authors: Knox Colin , Carmichael Paul 
Published: July 2017

Abstract
Local government in Northern Ireland has undergone a significant reform process in terms of both the number of councils (from twenty-six to eleven) and their functional responsibilities. Councils in Northern Ireland have always been regarded as the ‘poor relation’ of central government or non-departmental public bodies which deliver many of the services performed by local government in other parts of the UK (education, social services, housing). The reforms in Northern Ireland, while devolving relatively minor additional functions, offer councils a significant role in community planning – the legal power to hold central departments to account for services provided by them in local areas. This paper argues that councils can use this power to improve the quality of life of their inhabitants.
The phylogenetic analysis of Dalbergia (Fabaceae: Papilionaceae) based on different DNA barcodes
Authors: Li Qiwei , Wu Jihong , Wang Yesheng , Lian Xiaoming , Wu Feilong , Zhou Lin , Huang Zebo , Zhu Shuang 
Published: July 2017

Abstract
The genus Dalbergia contains approximately 250 species with many valuable trees being destroyed by targeted and illegal logging. DNA barcoding is a reliable method for the molecular identification of different species and resources conservation. In the present study, the specimen discrimination ability of internal transcribed spacer (ITS), matK, rbcL and psbA-trnH barcoding were tested on Dalbergia sequences, downloaded from the National Center for Biotechnology Information (NCBI), and the combined barcoding ITS+matK+rbcL was used to identify unknown specimens. It was found that ITS+matK+rbcL have good discrimination rates based on the analysis methods best match (BM) and best close match (BCM). These barcodes also have the best performance concerning barcode gap distribution, and are able to discriminate unknown specimens from South-China. Furthermore, it was demonstrated that D. tamarindifolia and D. rubiginosa are also relatively close to sister-species D. pinnata and D. candenatensis within the phylogenetic Dalbergia tree. Considering the overall performance of these barcodes, we suggest that the ITS+matK+rbcL region is a suitable barcode for identifying Dalbergia species.
Portable XRF on Prehistoric Bronze Artefacts: Limitations and Use for the Detection of Bronze Age Metal Workshops
Authors: Nørgaard Heide Wrobel 
Published: July 2017

AbstractTwo different scientific analyses-one destructive and one non-destructive-were conducted on two separate groups of bronze ornaments dating from 1500-1100 BC to investigate, amongst other traits, the metal composition of their copper-tin alloys. One group of artefacts was sampled, and polished thin sections were analysed using a scanning electron microscope (SEM). Results from the corrosion crust of copper-tin alloys, and the change measured within the elemental composition from the bulk metal to the surface, greatly influenced the interpretation of the second data set, which was measured using a handheld X-ray fluorescence (XRF) device. The surface of corroded bronze ornaments consists mostly of copper carbonates, oxides, and chlorides. Chemical processes, such as decuprification, change the element composition in such a manner that the original alloy cannot be traced with a non-destructive method. This paper compares the results of both investigations in order to define the possibilities and limits of non-destructive XRF analyses of corroded bronze artefacts.
National Strength and Conditioning Association's Endorsement of the National Collegiate Athletic Association Sport Science Institute's “Mental Health Best Practices: Inter-Association Consensus Document: Best Practices for Understanding and Supporting Student-Athlete Mental Wellness”
Authors: Gearity Brian , Moore E. Whitney G. 
Published: July 2017

ABSTRACT
COLLEGIATE ATHLETES EXPERIENCE UNIQUE AND SIGNIFICANT STRESSORS THAT REQUIRE AN INFORMED, TEAM APPROACH TO SUPPORTING THEIR MENTAL HEALTH. AS INTEGRAL MEMBERS OF THE SUPPORT TEAM RESPONSIBLE FOR COLLEGIATE ATHLETES' HEALTH AND WELL-BEING, STRENGTH AND CONDITIONING PROFESSIONALS SHOULD UNDERSTAND MENTAL HEALTH BEST PRACTICES AND APPLY THEM WITHIN THEIR SCOPE OF PRACTICE. THUS, THE NATIONAL STRENGTH AND CONDITIONING ASSOCIATION ENDORSES THE NATIONAL COLLEGIATE ATHLETIC ASSOCIATION'S DOCUMENT ENTITLED, “MENTAL HEALTH BEST PRACTICES: INTER-ASSOCIATION CONSENSUS DOCUMENT: BEST PRACTICES FOR UNDERSTANDING AND SUPPORTING STUDENT-ATHLETE MENTAL WELLNESS.” THE FOLLOWING PROVIDES A BRIEF REVIEW OF THIS DOCUMENT AND ITS IMPLICATIONS FOR STRENGTH AND CONDITIONING PROFESSIONALS.
Commitment-Based Strategies to Increase Exercise Participation
Authors: Kompf Justin , LaVaute Brittany 
Published: July 2017

ABSTRACT
PARTICIPATION IN EXERCISE IS STAGGERINGLY LOW AND FEW MEET THE RECOMMENDED PRESCRIPTIONS FOR AEROBIC AND RESISTANCE TRAINING. ONE REASON FOR MINIMAL PARTICIPATION MAY BE DUE TO NEGATIVE AFFECTIVE EXPERIENCES FELT DURING EXERCISE. THE USE OF BEHAVIORAL COMMITMENT STRATEGIES CAN SUPPORT INDIVIDUALS' EFFORTS WITH AN EXERCISE PROGRAM, DESPITE UNPLEASANT AFFECTIVE STATES.
Association between hypokalemia and cardiovascular diseases among hospitalized patients in tertiary care teaching hospital in North Karnataka: a retrospective study
Authors: Badiger Raju H. , Desai Santosh B. , Kantamanenin Ravindra , Kavatkopp Chetana K. , George Joel , Basha Sheikh H. 
Published: July 2017

Background: Potassium is an extremely crucial element in maintaining the normal charge between intracellular and extracellular space. Normal cellular function is maintained through potassium homeostasis. The normal range of plasma potassium is 3.5 to 5.1 mmol/L. The deviation both hypo and hyperkalaemia are associated with cardiovascular diseases especially cardiac arrhythmia. The objective was to study the association of hypokalaemia on cardiovascular diseases.Methods: During this one year of retrospective study socio-demographic profile of the patients were collected from the medical record section of hospital. The participants were grouped into four classes based on the serum potassium levels as, Group A: < 2 mmol/L, group B: 2-2.49 mmol/l, group C: 2.5 - 2.9 mmol/L, group D: 3 - 3.49 mmol/L and group E (control group) 3.5 to 5.1 mmol/L. The participants were also grouped as those suffering from cardiovascular diseases and those with non-cardiovascular diseases.Results: In our study 4818 records were categorized into 5 groups including control group. Males (61.35%) were slightly more than female (38.65%). The mean age group was 43±4 years. Results revealed that there was positive association between the sexes. Females were more commonly associated as compared to males. Hypokalaemia among cardiovascular diseases was only 22.2% as compared to non-cardiovascular diseases. The mean systolic (142±8) and diastolic Blood pressure (92±2) among cardiovascular diseases was slightly higher as compared with the non-cardiovascular diseases.Conclusions: Hypokalaemia is significantly associated with myocardial infarction when compared with other disorders. Hypokalaemia was more commonly associated with cardiovascular diseases as compared with non-cardiovascular diseases.
Evaluation of serum c-peptide levels in type 2 diabetics in Punjabi population
Authors: Deep Hardeep Singh , Singh Barjinder Pal , Singh Shalinder Pal 
Published: July 2017

Background: Diabetes mellitus is a chronic metabolic and endocrine disorder characterized by chronic hyperglycemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action or both. It is a major cause of mortality and morbidity worldwide. Human insulin and c-peptide are synthesized as a single polypeptide chain known as Proinsulin in the pancreatic beta cells. Serum insulin measurement gives a wrong value of insulin secretion, because insulin after its secretion into the portal vein, passes through the liver where approximately 50% of the delivered insulin is extracted. The measurement of C-peptide, thus provides a better index of endogenous insulin production and pancreatic beta cell function than insulin measurements.Methods: The present study was conducted on 100 adult patients of type 2 diabetes mellitus presenting in OPD and emergency or admitted in Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar. Type 1 diabetic patients, pregnant females with diabetes, patients presenting with acute infections, septicaemia, patients with acute or chronic pancreatitis, patients with pancreatic carcinoma were excluded from the study. In this study C-Peptide levels were estimated by DRG C-peptide ELISA method.Results: In our study, 38% patients had adequate insulin reserve (Normal C-peptide levels). Only 2% patients had poor insulin reserve (C-peptide levels below normal). 60% patients had c peptide levels more than normal, indicating insulin resistance. Increase in fasting c-peptide levels were associated with increased fasting plasma glucose (due to insulin resistance). A positive correlation exists in our study with r value of 0.523.Conclusions: As majority of patients with elevated FBS and fasting c-peptide were obese, our study infers that obese are more insulin resistant than non-obese. Since c-peptide levels assess the endogenous insulin reserve, it will also be helpful to alter the treatment modality based on it. So, routine c-peptide testing should be done in patients with poor glycaemic control to modify treatment modality accordingly.
A study of various factors associated with sexual dysfunction in males with type 2 diabetes mellitus
Authors: Jakka Nagendar Reddy , Ramesh2 Jayanthy 
Published: July 2017

Background: SD is very common in type 2 diabetic men. Many middle aged and older adults with diabetes are sexually active, but the rate of sexual inactivity is higher than in non-diabetic subjects. The objective of this study was to study various factors associated with sexual dysfunction in males with type- 2 diabetes mellitus.Methods: A total of 60 male type 2 diabetic subjects were studied. They were divided in to two groups, 42 subjects with SD were in one group and 18 subjects with normal sexual function were in the second group. Differences between groups were assessed for statistical significance using T-test, Mann-Whitney test and Chi-square test, where ever applicable. The data were presented as Mean±Standard deviation or percentages.Results: The prevalence of SD was 70%, ED was 93%, Orgasmic dysfunction was 38 %, 9 had HSDD (21%) and 22 had premature ejaculation (52%). The age (p = 0.335), duration of DM (p = 0.097), BMI (p = 0.717), WC (p = 0.138) were not significantly different between two groups. FPG (p = 0.000), PPG (p = 0.000) and HbA1c (p = 0.000) were significantly higher in SD group. Non-HDL cholesterol level (p = 0.001) and TG level (p = 0.021) were significantly higher in SD group. The eGFR (p = 0.150) was not significantly different between two groups. Spot urine protein creatinine ratio (p = 0.002) was significantly higher in SD group.Conclusions: It was concluded that SD is a highly prevalent problem in males with type 2 DM. ED is the most common form of SD, followed by PE, Orgasmic dysfunction and HSDD. The age, duration of DM, BMI and WC are not associated factors of SD.
Vitamin B12 deficiency among vegetarian and non-vegetarian diabetic population receiving prolonged Metformin based oral hypoglycemic agents therapy
Authors: Verma Vivek Kumar , Nim Ranjit Kumar , Singh P. S. , Kumar Manoj , Singh Geeta , Singh Anand Kumar 
Published: July 2017

Background: Diabetes is a group of metabolic disorder that share the phenotype of hyperglycemia. Over the period of time the metabolic dysregulation associated with diabetes mellitus causes secondary pathophysiological changes in multiple organs like heart, blood vessels, eyes, kidney and nerves resulting in various complications. However, a common potential interaction of metformin with vitamin B-12 is well documented but is poorly studied by the physicians who prescribe metformin to their diabetic patients. Since vitamin B-12 deficiency is common among vegan population as compared to population consuming food of animal origin (meat, fish and dairy products). But in this article, it has been studied that metformin cause vitamin B-12 deficiency even in non-vegetarian population. The aim of this study was to asses’ vitamin B12 deficiency among vegetarian and non-vegetarian diabetic population receiving prolonged Metformin based oral hypoglycaemic agents therapy. It was a cross sectional study done in Department of Medicine, UPUMS, Saifai, Etawah, Uttar Pradesh, India.Methods: Study done among patients of type 2 diabetes mellitus of age group 30-60 years on prolonged metformin based OHA therapy and having peripheral neuropathy were included in study. Data was analysed on SPSS Version 22.0 and p value obtained.Results: Statistical analysis of 93 patients included in study showed that vitamin B12 deficiency is common among the vegetarian (56.52%) and non-vegetarian (35.71%) population but the difference is not statistically significant (p value=0.29) which is more in favour of metformin associated vitamin B12 deficiency in non-vegetarian population.Conclusions: Vitamin B12 deficiency is common in diabetes patients on metformin based OHA therapy hence we recommend routine screening for Vitamin B12 deficiency in such diabetes patients.