bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbiota of spring wheat seeds grown within the contrasting agroclimatic conditions of the Tyumen region
Authors: Zemtsova E. S. , Bome N. A. , Novokhatin V. V. 
Published: October 2024

Relevance. Pathogenic microbiota of seed grain can cause death of seedlings, root rot, weakening of plants and a decrease in yield. Monitoring of seed grains pathogens infection rate is always relevant because the composition of the microbiota is dynamic due to the influence of natural and anthropogenic factors.Methods. Phytopathological analysis of spring wheat seeds grown at the state variety testing sites of the Tyumen region was carried out in contrasting years: cool and excessively moist in July 2015 and hot with moderate rainfall in 2016. We used the “wet chamber” method. 144 samples were analyzed.Results. Fungus of the genus Alternaria were the most abundant representatives of the grain microbial community. Average infection rate of seed samples with fungi Alternaria spp. was 49.5%. No differences were found by year of the study. In 2015 compared to 2016, higher prevalence of fungus of the genus Fusarium (4.8% vs. 1.6%) and bacteria (5.7% vs. 0.7%) was observed in the seed material. The maximum indicators of Fusarium infestation were observed in samples from Nizhnetavda variety testing site located in the subtaiga zone – up to 30% (variety Ikar). In 2016 compared to 2015, higher infection of seed material with helminthosporiose (7.1% vs. 2.6%) and mold fungi (2.3% vs. 0.2%) was observed. The prevalence of Bipolaris sorokiniana fungi was the highest in wheat samples grown in the northern forest-steppe zone, especially samples from the Yalutorovsky variety testing site — the limit value was 27% (variety SKENT-3). Harmfulness fungus of the genus Alternaria was significantly lower compared to other key representatives of grain microbiota. The average score of seedling damage was 1.4 vs. 2.2–2.6, seed germination indices — 94.1% vs. 53.2–67.4%.
The influence of climatic factors on the effect of growth regulators in agrocenoses of spring barley
Authors: Ryabtseva N. A. 
Published: October 2024

Relevance. In recent years, under the conditions of climate change, there has been a tendency to reduce the acreage of spring barley, reduce the quantity and quality of grain. From the point of view of ensuring the stability of gross grain harvests and ecology, it is necessary to manage the agrocenosis.Methods. The experiments were conducted in 2021–2024 agricultural years in the conditions of the Azov sea zone of the Rostov region. Objects of research: Leon spring barley variety and growth regulators “Artafit” (0.3 l/ha), “Biodux” (3 ml/ha), “Obereg” (60 ml/ha), “Trainer” (3 l/ha), “Fulvohumate” (0.4 l/ha).Results. The climatic conditions in 2024 were extreme in terms of low rainfall and high amounts of spring temperatures. The year 2023 was the most favorable from this point of view. The yield level correlates with the level of moisture in the spring period, the highest indicators were observed in 2023, and the lowest in 2024. In the current market conditions and annual price increases, unit production costs are increasing. Thus, in 2024, the cultivation of spring barley turned out to be unprofitable. Moreover, the use of biological products did not allow us to gain a plus. In 2022 and 2023, the use of the drug “Trainer” turned out to be unprofitable due to the high cost of the drug. “Biodux” and “Obereg” showed the highest profitability.
Qualitative indicators of milk and fermented milk products when activated zeolite and probiotics are included in the diet of cows
Authors: Krupin E. O. , Shakirov Sh. K. , Gainullina M. K. , Bikchantaev I. T. , Davletshina G. A. , Shavaleev M. F. , Hoggui M. , Antonov M. V. , Khairullina A. R. , Askarova A. A. , Kosenkova O. Sh. 
Published: October 2024

Relevance. The quality of milk depends on the season of the year, the breed of animals, the state of metabolism, and the characteristics of feeding and maintenance technology. The quality of milk determines its technological properties and the quality of its processed products.Methods. The content of somatic cells in milk, the acidity of milk, cottage cheese, yogurt, and syneresis of yogurt were determined. In milk processing products, the content of the mass fraction of nitrogen and crude protein, crude ash, calcium, and phosphorus was determined.Results. When feeding cows with the yeast probiotic activated zeolite and the phytoprobiotic “Provitol”, a significant decrease in the content of somatic cells in freshly milked milk of cows was established by 13.02% (p < 0.05). Significantly lower milk acidity values were established (by 6.00% (p < 0.001) and 12.79% (p < 0.001), respectively), significantly higher yogurt acidity values (by 6.00% (p < 0.001) and 12.79% (p < 0.001), respectively, significantly lower yogurt syneresis (by 4.70% (p < 0.001) and 3.10% (p < 0.01), respectively). The use of activated zeolite and yeast probiotic “Kluver Pro” in the diets leads to a significantly higher content (compared with the control) of the mass fraction of fat in cottage cheese and yogurt — by 10.47 abs. % (p < 0.001) and 4.58 abs. % (p < 0.001).
STRATEGI GURU DALAM PENERAPAN PROGRAM SNACK SEHAT UNTUK PENCEGAHAN STUNTING PADA ANAK USIA DINI
Authors: Ria Octa Viana  , Maria Qori’ah  
Published: October 2024

Program pemberian snack sehat anak usia dini merupakan program yang dilakukan dalam usaha pencegahan stunting pada Anak usia dini. Melalui makanan yang menarik dan bergizi, sehingga membuat anka tertarik akan makanan tersebut yang dimana akna berpengaruh pada tumbuh kembang anakmeningkat secara optimal. TK Khadijah 21 Tegaldlimo sudah melaksanakan program snack sehat dalam pencegahan stunting pada anak usia khususnya usia 4-5 tahun, salah satu pencetus pelaksanaan program ini dikarenakan adanya stunting karena penurunan berat badan dan tinggi badan yang tidak ideal dengan usianyapada beberapa peserta didik, dalam hal ini program snack sehat sangat efektif diterapkan. Penelitian ini menggunakan pendekatan kualitatif deskriptif dengan jenis penelitian studi kasus. Penelitian ini menggunakan wawancara mendalam, observasi partisipatif, dan dokumentasi. Analisis data menggunakan kondensasi data, display data, penarikan kesimpulan dan verifikasi. Sedangkan keabsahan data menggunakan triangulasi sumber dan Teknik. Dari hasil penelitian ini dapat disimpulkan bahwa, program snack sehat anak usia dini dalam pencegahan stunting sangat optimal untuk dilakukan. Melalui makanan yang bergizi, tumbuh kembang dan kognitif anak akan sangat berkembang dengan optimal. Hal ini di dukung dengan penerapan langkah-langkah Program Snack sehat anak usia dini dalam pencegahan stunting yaitu perencanaan, pelaksanaan, dan evaluasi. Melalui langkah-langkah tersebut, penerapan program snack sehat dapat terlaksana secara optimal dan terencana sesuai tujuan
City Centre Car Parking Provision in Liverpool
Authors: Nurse Alexander 
Published: October 2024

This paper presents an inventory of car parking provision in the city centre of Liverpool, UK. Using a systematic approach which determines car-parking spaces through publicly available data, manual counts of satellite imagery and inference to determine maximal potential capacity, site visits, and, and planning records, the study identified 34,585 parking spaces in Liverpool City Centre. This is analysed in terms of public parking, commercial parking (e.g. staff/customer-only), and resident parking. Using census data, the study suggests that Liverpool city centre has ample supply and, perhaps, a modest over supply of car parking spaces to meet potential demand.
Liposomes – metabolically active drug transport systems: classification, components, preparation methods, and stabilization. Part 1 (review)
Authors: Osochuk S. S. , Kotsur Yu. M. , Pozharitskaya O. N. , Flisyuk E. V. , Smekhova I. E. , Malkov S. D. , Zarifi K. O. , Titovich I. A. , Krasova E. K. , Shikov A. N. 
Published: October 2024

Introduction. This review article is focused on the modern classification of liposomes, preparation methods, stabilization and the role of structural components, visualization, and pharmacokinetics. Part 1 discusses the first three aspects mentioned above.Text. Depending on the size and number of bilayers, liposomes are classified into simple, long-circulating, cationic, immuno-liposomes, and sterically stabilized. The lipid components of liposomes can have a pronounced effect on target organs and tissues. Metabolites of the main components of liposomes have their biological activity, depending on their combination and dosage. All of the above indicates the promise of using liposomes not only as carriers of drugs but also as independent effectors that can have a significant impact on human metabolism in various diseases. The advantages and limitations of methods for preparing liposomes, and the features for manufacturing stealth liposomes are discussed. A special section of the article is dedicated to liposome stabilization.Conclusions. The information discussed in the review article may be useful in the development of pharmaceutical formulations in liposomes. Liposomes are not only promising nanocontainers for targeted drug delivery, but also metabolically active complexes with a wide spectrum of activity.
Patient autonomy in self-managing their bronchial asthma exacerbation and its associated factors, 2024
Authors: Abdulwehab Sadik , Kedir Frezer 
Published: October 2024

IntroductionAsthma, a global chronic respiratory condition, varies in patient autonomy due to limited resources, health literacy, and cultural beliefs, emphasizing the importance of understanding this autonomy for improved asthma management.MethodsA cross-sectional study was conducted at Jimma University Comprehensive Specialized Hospital, involving face-to-face interviews with 175 patients. Data was collected on sociodemographic characteristics, clinical factors, and autonomy levels using a validated Patient Autonomy Preference Index. Descriptive statistics and binary logistic regression analysis were used.ResultsA total of 175 participants were recruited, out of them 41.7% (95% CI: 31.19, 40.41)) of participants were autonomous in managing their asthma exacerbation. 127 (72.6%) of study participants were female, with a mean age of 47.51 (SD ± 13.96), 101(57.7%) were live in urban areas, 54 (30.9%) had no formal education, 140 (80%) were married, 112 (64%) had health insurance, and 102(83.3%) obtained health information about their condition from healthcare workers. Reside in an urban area (AOR = 3.24; 95% CI: 1.40–7.49, p &lt; 0.006), have health insurance (AOR = 4.30; 95% CI: 1.76–10.51, p &lt; 0.001), those doing regular exercise (AOR = 4.79; 95% CI: 1.69–13.64, p &lt; 0.003), have family history (AOR = 7.47; 95% CI: 1.61–34.60, p &lt; 0.01), have a duration above five years since diagnosis (AOR = 0.44; 95% CI: 1.04–1.26, p &lt; 0.003), and participants with a high level of health literacy (AOR = 1.10; 95% CI: 1.00–1.20, p &lt; 0.042) become associated with being autonomous in managing asthma exacerbation.Conclusion and recommendationOnly around forty-two percent of study participants were autonomous in managing their asthma exacerbation. Thus healthcare providers should give due attention to those who reside in rural areas, are not insured, recently diagnosed with asthma, and with low health literacy to enhance patient autonomy and self-management practices, ultimately improving health outcomes for individuals with asthma.
A complementary approach for detecting biological signals through a semi-automated feature selection tool
Authors: Arini Gabriel Santos , Mencucini Luiz Gabriel Souza , de Felício Rafael , Feitosa Luís Guilherme Pereira , Rezende-Teixeira Paula , de Oliveira Tsuji Henrique Marcel Yudi , Pilon Alan Cesar , Pinho Danielle Rocha , Costa Lotufo Letícia Veras , Lopes Norberto Peporine , Trivella Daniela Barretto Barbosa , da Silva Ricardo Roberto 
Published: October 2024

IntroductionUntargeted metabolomics is often used in studies that aim to trace the metabolic profile in a broad context, with the data-dependent acquisition (DDA) mode being the most commonly used method. However, this approach has the limitation that not all detected ions are fragmented in the data acquisition process, in addition to the lack of specificity regarding the process of fragmentation of biological signals. The present work aims to extend the detection of biological signals and contribute to overcoming the fragmentation limits of the DDA mode with a dynamic procedure that combines experimental and in silico approaches.MethodsMetabolomic analysis was performed on three different species of actinomycetes using liquid chromatography coupled with mass spectrometry. The data obtained were preprocessed by the MZmine software and processed by the custom package RegFilter.Results and DiscussionRegFilter allowed the coverage of the entire chromatographic run and the selection of precursor ions for fragmentation that were previously missed in DDA mode. Most of the ions selected by the tool could be annotated through three levels of annotation, presenting biologically relevant candidates. In addition, the tool offers the possibility of creating local spectral libraries curated according to the user’s interests. Thus, the adoption of a dynamic analysis flow using RegFilter allowed for detection optimization and curation of potential biological signals, previously absent in the DDA mode, being a good complementary approach to the current mode of data acquisition. In addition, this workflow enables the creation and search of in-house tailored custom libraries.
Right anterior thoracotomy vs. upper hemisternotomy for aortic valve replacement with Perceval S: is there a difference?
Authors: Okiljevic Bogdan , Raickovic Tatjana , Zivkovic Igor , Vukovic Petar , Milicic Miroslav , Stojanovic Ivan , Milacic Petar , Micovic Slobodan 
Published: October 2024

BackgroundOur study aimed to evaluate the early outcomes of aortic valve replacement with Perceval S sutureless valve through the right anterior thoracotomy and upper hemisternotomy approaches, and to determine if there are any differences between these two approaches.MethodsWe carried out a study using data from 174 patients who underwent minimally invasive Perceval S valve implantation for aortic valve stenosis between January 2018 and August 2023. This was a retrospective, single-center observational study. The patients were divided into two groups: the hemisternotomy group (n = 100) and the right anterior thoracotomy group (n = 74).ResultsThe overall in-hospital mortality was 1,7%. The cardiopulmonary bypass and cross-clamp times were longer in the right anterior thoracotomy group (p &lt; .001). There were no statistically significant differences in terms of stroke, paravalvular leak, mechanical ventilation time, blood transfusion requirements, pacemaker implantation, reexploration for bleeding, conversion, wound infection, or in-hospital stay. Postoperative chest drainage was lower (p &lt; .001) and postoperative atrial fibrillation occurred less frequently (p = .044) in the right anterior thoracotomy group. The median intensive care unit stay was shorter in the right anterior thoracotomy group (p = .018).ConclusionAortic valve replacement with the Perceval S valve through either an upper hemisternotomy or a right anterior thoracotomy is a procedure associated with low perioperative complication rates. Right anterior thoracotomy for an aortic valve replacement with the Perceval S valve was associated with lower postoperative bleeding, a lower postoperative atrial fibrillation incidence and a shorter intensive care unit stay compared to upper hemistornotomy.
Cardioprotection in coronary artery bypass graft surgery: the impact of remote ischemic preconditioning on modulating LOX-1 and SOD-1 to counteract oxidative stress
Authors: Luca Cezar-Dumitrel , Boieriu Alexandra , Neculoiu Daniela , Țînț Diana 
Published: October 2024

BackgroundCoronary artery bypass grafting (CABG) is frequently used to treat severe coronary artery disease (CAD), but it can lead to increased oxidative stress and inflammation, worsening patient outcomes. Remote ischemic preconditioning (RIPC) has been suggested as a potential strategy to protect against these effects by modulating oxidative stress and inflammatory responses, though its impact on specific biomarkers requires further investigation. This study aims to assess the effects of remote ischemic preconditioning on inflammation markers and oxidative stress in patients with severe CAD undergoing coronary artery bypass grafting.MethodsWe conducted a case-control study involving 80 patients with severe coronary artery disease (CAD) scheduled for coronary artery bypass grafting (CABG). Fifty percent of these patients received ischemic preconditioning prior to surgery. Plasma levels of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and Superoxide dismutase-1 (SOD-1) levels were measured in all individuals using the ELISA method at three important time points: before surgery (visit 1 or V1), immediately post-operatively (visit 2 or V2), and one week post-operatively (visit 3 or V3).ResultsWe enrolled 80 patients, of which 40 were assigned to the studied group receiving remote ischemic preconditioning (RIPC) and 40 to the control group. There were no statistically significant differences between the groups regarding baseline, clinical, or operative characteristics. RIPC treatment significantly reduced plasma levels of Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1) (p &lt; 0.05) as well as significantly increasing total values of Superoxide dismutase-1 (SOD-1) (p &lt; 0.05, respectively). There were notable differences between the studied and control groups at V2 and V3. The studied group had higher SOD-1 levels (p &lt; 0.05) and significantly lower LOX-1 levels at both time points (p &lt; 0.05).ConclusionThe significant changes in plasma levels of both LOX-1 and SOD-1 observed in this study strongly suggest that remote ischemic preconditioning (RIPC) plays an important role in reducing oxidative stress and enhancing the antioxidative status of patients. This is evidenced by the marked decrease in LOX-1 levels, alongside a corresponding increase in SOD-1 levels, indicating that RIPC may contribute to improved cardioprotection through these mechanisms.
Effectiveness of household-level interventions for reducing the impact of air pollution on health outcomes – a systematic review
Authors: Salve Harshal Ramesh , Nawaz Huma , Dey Sagnik , Krishnan Anand , Sharma Preeti , Madan Karan 
Published: October 2024

Background and aimSeveral air pollution mitigation initiatives have been researched worldwide to see how efficient they are at reducing air pollution and the related health benefits. The effectiveness of these interventions for improving health outcomes is key for scaling up and increasing uptake of same by policymakers and people. We aimed to synthesize scientific evidence about the effectiveness of household-level interventions on air quality and health outcomes using a systematic approach.MethodsComprehensive strategy was used to carry out a literature search in PubMed, Cochrane, and Google scholar by two independent investigators using keywords and Medical Subject Heading terms. Research articles published till the year 2021 focusing on interventions for improvement in air quality and health were selected using pre-specified eligibility criteria. All abstracts for inclusion were reviewed independently by two reviewers. In case of doubt, the articles were included for full-text review. Disagreements were resolved by discussion with the third investigator. The quality of the studies and risk of bias was assessed using the Joanna Briggs Institute critical appraisal tool and Cochrane Risk of Bias tool, version 2.0. Effect estimates were reported using odds ratio, relative risks, difference in mean, and percentage changes with 95% confidence intervals. PRISMA guidelines were used to conduct and report the systematic review. The review was registered in PROSPERO (CRD42022295393).ResultsThe search resulted in the identification of 46,809 studies and 18 of them were finally included in the review using eligibility criteria. The household-level interventions were categorized mainly into air purifiers/electric heater use, cookstove use, and behavioural change interventions. These interventions resulted in a decrease in the number of daytime asthma attacks by 33%, an increase in FEV1 by 4.4%, −1.4% and 0.8% overall percent change in microvascular and lung function. PM2.5 was the most commonly reported air pollutant, its concentrations reduced in the range between 31% and 53% across the studies.ConclusionOur review provided consistent evidence for the effectiveness of household-level interventions thus associated with improvements in cardiovascular and respiratory symptoms.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=295393, identifier, CRD42022295393
Utilization and determinants of maternity waiting homes among pastoralist mothers in Dire district, southern Ethiopia: a mixed-methods study
Authors: Duba Hassan Mahamad , Mekuria Mulugeta , Malka Erean Shigign , Tadesse Addisu Waleligne , Gashaw Ketema , Eshetu Ketema 
Published: October 2024

BackgroundMaternity waiting homes are cost-effective, World Health Organization-approved components of comprehensive prenatal, delivery, and postpartum care strategies. However, few community-based studies within Ethiopia's pastoralist communities, and none in the study area, have been conducted to determine actual usage or to gain a thorough understanding of the factors influencing utilization.MethodsA cross-sectional study, supplemented by qualitative methods, was conducted from June 25 to July 25, 2023. A simple random sampling technique was used to select 305 study participants. Data were gathered through an interviewer-administered questionnaire, entered into Epi-data version 3.1, and analyzed using SPSS version 25. Descriptive data were presented in tables, graphs, text, and percentages. Bivariate logistic regression identified candidate predictors at a P-value of &lt;0.25, and predictors of maternity waiting home utilization were identified through multivariate logistic regression at a 95% confidence interval and P-value of &lt;0.05. Qualitative interviews were transcribed, translated, and thematically analyzed.ResultsThe prevalence of maternity waiting home use in the study area was 35.2% (95% CI: 30.9%, 39.5%). Factors associated with maternity waiting home utilization included travel time greater than 60 min to nearby health facilities (AOR: 5.47 CI: 1.77, 16.91), good knowledge of danger signs of pregnancy (AOR: 5.41, CI: 1.86, 15.79), lack of a caretaker to household tasks (AOR: 0.1, CI: 0.03, 0.31), and a refusal to accept a waiting time of 2–4 weeks (AOR: 0.24 CI: 0.08, 0.74). The qualitative findings underscored hurdles such as resource constraints, challenges in providing maternity services, and the importance of community awareness and access to network connectivity in ensuring safe childbirth.ConclusionThis study aims to determine the utilization of maternity waiting homes and the factors associated with their use among women who gave birth within the last year in the Dire district, Borana zone, southern Ethiopia. The prevalence of maternity waiting home use was low compared to national efforts to promote this service. Longer travel time, lack of a caretaker, good knowledge of danger signs of pregnancy, and a refusal to accept a waiting time of 2–4 weeks were associated with maternity waiting home use in this study.