bluuebunny/crossref_metadata_2025_split huggingface dataset:
Legionella longbeachaedetected in an industrial cooling tower linked to a legionellosis outbreak, New Zealand, 2015; possible waterborne transmission?
Authors: THORNLEY C. N. , HARTE D. J. , WEIR R. P. , ALLEN L. J. , KNIGHTBRIDGE K. J. , WOOD P. R. T. 
Published: June 2017

SUMMARYA legionellosis outbreak at an industrial site was investigated to identify and control the source. Cases were identified from disease notifications, workplace illness records, and from clinicians. Cases were interviewed for symptoms and risk factors and tested for legionellosis. Implicated environmental sources were sampled and tested for legionella. We identified six cases with Legionnaires’ disease and seven with Pontiac fever; all had been exposed to aerosols from the cooling towers on the site. Nine cases had evidence of infection with eitherLegionella pneumophilaserogroup (sg) 1 orLegionella longbeachaesg1; these organisms were also isolated from the cooling towers. There was 100% DNA sequence homology between cooling tower and clinical isolates ofL. pneumophilasg1 using sequence-based typing analysis; no clinicalL. longbeachaeisolates were available to compare with environmental isolates. Routine monitoring of the towers prior to the outbreak failed to detect any legionella. Data from this outbreak indicate thatL. pneumophilasg1 transmission occurred from the cooling towers; in addition,L. longbeachaetransmission was suggested but remains unproven.L. longbeachaedetection in cooling towers has not been previously reported in association with legionellosis outbreaks. Waterborne transmission should not be discounted in investigations for the source ofL. longbeachaeinfection.
Consistency matters: Consistency in the timing and quality of daily interactions between parents and adolescents predicts production of proinflammatory cytokines in youths
Authors: Manczak Erika M. , Leigh Adam K. K. , Chin Chia-Ping , Chen Edith 
Published: June 2017

AbstractThe current study examined whether consistency in day-to-day interactions between children and parents related to inflammatory cytokine production in youths. One hundred twenty-three parents recorded the daily quality of interactions and timing of leisure activities with their adolescent children for 2 weeks, and the degree of variability in those ratings was calculated. One year later, the production of proinflammatory cytokines in youths’ blood was measured in response to in vitro exposure to lipopolysaccharide (a bacterial product). The results indicate that greater variability in parent–child relationship quality related to greater stimulated proinflammatory cytokine production in youths, above and beyond overall relationship quality. Greater variability in the timing of parent–child leisure activities also predicted greater stimulated proinflammatory cytokine production in youths, regardless of the frequency of interactions. In sum, consistency in both the affective and temporal aspects of parent–child relationships may contribute to inflammatory processes in youth.
How does fatigue management education impact radiographer competence and confidence in supporting patients during radiotherapy?
Authors: Tolson L. , Jessop A. 
Published: June 2017

AbstractAimThe primary outcome of this study is to establish whether fatigue management education impacts on radiographers’ perceived competence and confidence in supporting patients undergoing radiotherapy.Materials and methodsA single-centre mixed method study was conducted. Participants completed a questionnaire determining baseline fatigue support practice, undertook an education package, then repeated the questionnaire to determine any change in their competence and confidence. Semi-structured interviews were used to gain insight into practice and perceived barriers.ResultsIn all, 17 radiographers (100%) participated. Some areas of practice were affected significantly by the education package, namely highlighting the need for training, perceived competence and confidence in providing support, knowledge of effective treatment options and risk factors, the effect of fatigue on the patient and greater agreement that their professional qualification means they are qualified to support patients with fatigue.FindingsFatigue management education impacts on radiographers’ perceived competence and confidence in supporting patients during their radiotherapy. Interviews highlighted that knowledge of cancer-related fatigue is built up through day-to-day practice. Although there is greater awareness and support of fatigue, barriers exist, including patient compliance, lack of practical training, provision to monitor patients and unclear referral pathways.
Do not confuse multidisciplinary task management in nursing homes with interprofessional care!
Authors: Tsakitzidis Giannoula , Anthierens Sibyl , Timmermans Olaf , Truijen Steven , Meulemans Herman , Van Royen Paul 
Published: June 2017

Problem statement
Little is known about how interprofessional healthcare providers in nursing homes work together. We know that interprofessional teamwork evolves from trial and error learning and so interprofessional collaboration has to be actively taught. This study aims to gain insights in the perception of professionals towards interprofessional collaboration in nursing homes and the factors that have an impact on interprofessional collaboration.

Approach
A qualitative descriptive methodology using focus group interviews and additional semi-structured interviews was performed. In total three focus group sessions with healthcare providers from different disciplines were held and additionally nine semi-structured interviews were executed. A thematic analysis was performed. The transcripts were read to immerse in the data and initial ideas were noted. Both open coding (identification of primary themes) and axial coding (analysis of relationships among themes) were conducted and re-focussed into potential themes.

Findings
Four main themes emerge from the analysis: context, collaboration, care and experience. From the findings it seems that healthcare teams in nursing homes work as ‘separated groups’. A lot of collaboration is perceived, but no common vision or responsibility sharing is found. The role description of the different disciplines does not always seem clear or is not always explicit.

Conclusion
In usual care the perceived interactions between professionals are called collaboration. Obviously physicians and all healthcare professionals do not work interprofessionally according to definitions from the literature. This study provided evidence of the awareness that interprofessional collaboration in usual care is situational and fragmentary organised.
Logic + control: On program construction and verification
Authors: DRABENT WŁODZIMIERZ 
Published: June 2017

AbstractThis paper presents an example of formal reasoning about the semantics of a Prolog program of practical importance (the SAT solver of Howe and King). The program is treated as a definite clause logic program with added control. The logic program is constructed by means of stepwise refinement, hand in hand with its correctness and completeness proofs. The proofs are declarative – they do not refer to any operational semantics. Each step of the logic program construction follows a systematic approach to constructing programs which are provably correct and complete. We also prove that correctness and completeness of the logic program is preserved in the final Prolog program. Additionally, we prove termination, occur-check freedom and non-floundering.Our example shows how dealing with “logic” and with “control” can be separated. Most of the proofs can be done at the “logic” level, abstracting from any operational semantics.The example employs approximate specifications; they are crucial in simplifying reasoning about logic programs. It also shows that the paradigm of semantics-preserving program transformations may be not sufficient. We suggest considering transformations which preserve correctness and completeness with respect to an approximate specification.
CPW-fed SGF-TSRR antenna for multiband applications
Authors: Elavarasi C. , Shanmuganantham T. 
Published: June 2017

In this paper, a solid co-planar waveguide-fed fractal metamaterial antenna is offered. The proposed design consists of Sierpinski gasket iterations and a complementary triangular split ring resonator (CTSRR) loaded underneath the substrate, which is accountable for pull off multiband uniqueness and resolve good impedance identical. In sketch to hassle multiresonant frequency band facet, these CTSRR are entrenched reverse side of the substrate. The anticipated antenna with a dense dimension of 12 × 14 × 1.6 mm3 is fabricated and tested. The testing result designates that the projected design has −10 dB of 5.72, 14.3, and 16.06 GHz, respectively, and covers 5.72 GHz wireless local area network, 14.3 GHz fixed satellite, and 16.06 GHz International Telecommunication Union (ITU) band. It has fine emission uniqueness for jointly E-plane and H-plane in all the preferred occurrence bands and produce superior performance compared with the offered antenna intend in the prose. The loaded CTSRR construction recital is validated all the way during negative permeability pulling out and assorted parametric study.
The bile acid receptor FXR attenuates acinar cell autophagy in chronic pancreatitis
Authors: Zhou Xiaodong , Xie Li , Bergmann Frank , Endris Volker , Strobel Oliver , Büchler Markus W , Kroemer Guido , Hackert Thilo , Fortunato Franco 
Published: June 2017

AbstractThe functional relationship between bile acid (BA) and autophagy has not been evaluated in the context of pancreatitis. Here we investigated whether BA and their nuclear farnesoid X receptor (FXR) modulate autophagy and the development of pancreatitis. FXR expression, autophagy, apoptosis and necroptosis were determined in human chronic pancreatitis (CP) tissue in vivo and in pancreatic cells lines in vitro by means of real-time PCR, immunoblots and immunofluorescence. Pancreatic cell lines exposed to the most abundant BAs glycochenodeoxycholate (GCDC) and taurocholic acid (TCA) increased the expression of nuclear FXR and diminished that of the essential autophagy-related protein ATG7. BA was also elevated in pancreatic tissues from CP patients, correlating with elevated FXR and curtailed ATG7 expression with locally reduced autophagic activity. This was accompanied by an increased manifestation of CP hallmarks including apoptosis, necroptosis, inflammation and fibrosis. The present results suggest a cascade of events in which local accumulation of BA signals via FXR to suppress autophagy in pancreatic acinar cells, thereby unleashing acinar cell apoptosis and necroptosis. Thus, BA may cause CP by suppressing autophagy and exacerbating acinar cell apoptosis and necroptosis.
Fronts and waves of actin polymerization in a bistability-based mechanism of circular dorsal ruffles
Authors: Bernitt Erik , Döbereiner Hans-Günther , Gov Nir S. , Yochelis Arik 
Published: June 2017

AbstractDuring macropinocytosis, cells remodel their morphologies for the uptake of extracellular matter. This endocytotic mechanism relies on the collapse and closure of precursory structures, which are propagating actin-based, ring-shaped vertical undulations at the dorsal (top) cell membrane, a.k.a. circular dorsal ruffles (CDRs). As such, CDRs are essential to a range of vital and pathogenic processes alike. Here we show, based on both experimental data and theoretical analysis, that CDRs are propagating fronts of actin polymerization in a bistable system. The theory relies on a novel mass-conserving reaction–diffusion model, which associates the expansion and contraction of waves to distinct counter-propagating front solutions. Moreover, the model predicts that under a change in parameters (for example, biochemical conditions) CDRs may be pinned and fluctuate near the cell boundary or exhibit complex spiral wave dynamics due to a wave instability. We observe both phenomena also in our experiments indicating the conditions for which macropinocytosis is suppressed.
Cooperative tumour cell membrane targeted phototherapy
Authors: Kim Heegon , Lee Junsung , Oh Chanhee , Park Ji-Ho 
Published: June 2017

AbstractThe targeted delivery of therapeutics using antibodies or nanomaterials has improved the precision and safety of cancer therapy. However, the paucity and heterogeneity of identified molecular targets within tumours have resulted in poor and uneven distribution of targeted agents, thus compromising treatment outcomes. Here, we construct a cooperative targeting system in which synthetic and biological nanocomponents participate together in the tumour cell membrane-selective localization of synthetic receptor-lipid conjugates (SR-lipids) to amplify the subsequent targeting of therapeutics. The SR-lipids are first delivered selectively to tumour cell membranes in the perivascular region using fusogenic liposomes. By hitchhiking with extracellular vesicles secreted by the cells, the SR-lipids are transferred to neighbouring cells and further spread throughout the tumour tissues where the molecular targets are limited. We show that this tumour cell membrane-targeted delivery of SR-lipids leads to uniform distribution and enhanced phototherapeutic efficacy of the targeted photosensitizer.
DOT1L safeguards cartilage homeostasis and protects against osteoarthritis
Authors: Monteagudo Silvia , Cornelis Frederique M. F. , Aznar-Lopez Carolina , Yibmantasiri Ploi , Guns Laura-An , Carmeliet Peter , Cailotto Frédéric , Lories Rik J. 
Published: June 2017

AbstractOsteoarthritis is the most prevalent and crippling joint disease, and lacks curative treatment, as the underlying molecular basis is unclear. Here, we show that DOT1L, an enzyme involved in histone methylation, is a master protector of cartilage health. Loss of DOT1L disrupts the molecular signature of healthy chondrocytesin vitroand causes osteoarthritis in mice. Mechanistically, the protective function of DOT1L is attributable to inhibition of Wnt signalling, a pathway that when hyper-activated can lead to joint disease. Unexpectedly, DOT1L suppresses Wnt signalling by inhibiting the activity of sirtuin-1 (SIRT1), an important regulator of gene transcription. Inhibition of SIRT1 protects against osteoarthritis triggered by loss of DOT1L activity. Modulating the DOT1L network might therefore be a therapeutic approach to protect the cartilage against osteoarthritis.
Two-step synthesis of chiral fused tricyclic scaffolds from phenols via desymmetrization on nickel
Authors: Kumar Ravindra , Hoshimoto Yoichi , Tamai Eri , Ohashi Masato , Ogoshi Sensuke 
Published: June 2017

AbstractTricyclic furan derivatives with multiple chiral centers are ubiquitous in natural products. Construction of such tricyclic scaffolds in a stereocontrolled, step-economic, and atom-economic manner is a key challenge. Here we show a nickel-catalyzed highly enantioselective synthesis of hydronaphtho[1,8-bc]furans with five contiguous chiral centers via desymmetrization of alkynyl-cyclohexadienone by oxidative cyclization and following formal [4 + 2] cycloaddition processes. Alkynyl-cyclohexadienone was synthesized in one step from easily accessible phenols. This reaction represents excellent chemo-selectivity, regio-selectivity, diastereo-selectivity, and enantio-selectivity (single diastereomer, up to 99% ee). An extraordinary regioselectivity in the formal [4 + 2] cycloaddition step with enones revealed the diverse reactivity of the nickelacycle intermediate. Desymmetrization of alkynyl-cyclohexadienones via oxidative cyclization on nickel was supported by the isolation of a nickelacycle from a stoichiometric reaction. Enantioenriched tricyclic products contain various functional groups such as C=O and C=C. The synthetic utility of these products was demonstrated by derivatization of these functional groups.
Effects of energetic ion irradiation on WSe2/SiC heterostructures
Authors: Shi Tan , Walker Roger C. , Jovanovic Igor , Robinson Joshua A. 
Published: June 2017

AbstractThe remarkable electronic properties of layered semiconducting transition metal dichalcogenides (TMDs) make them promising candidates for next-generation ultrathin, low-power, high-speed electronics. It has been suggested that electronics based upon ultra-thin TMDs may be appropriate for use in high radiation environments such as space. Here, we present the effects of irradiation by protons, iron, and silver ions at MeV-level energies on a WSe2/6H-SiC vertical heterostructure studied using XPS and UV-Vis-NIR spectroscopy. It was found that with 2 MeV protons, a fluence of 1016 protons/cm2 was necessary to induce a significant charge transfer from SiC to WSe2, where a reduction of valence band offset was observed. Simultaneously, a new absorption edge appeared at 1.1 eV below the conduction band of SiC. The irradiation with heavy ions at 1016 ions/cm2 converts WSe2 into a mixture of WOx and Se-deficient WSe2. The valence band is also heavily altered due to oxidation and amorphization. However, these doses are in excess of the doses needed to damage TMD-based electronics due to defects generated in common dielectric and substrate materials. As such, the radiation stability of WSe2-based electronics is not expected to be limited by the radiation hardness of WSe2, but rather by the dielectric and substrate.
A microRNA signature of response to erlotinib is descriptive of TGFβ behaviour in NSCLC
Authors: Krentz Gober Madeline , Collard James P. , Thompson Katherine , Black Esther P. 
Published: June 2017

AbstractOur previous work identified a 13-gene miRNA signature predictive of response to the epidermal growth factor receptor (EGFR) inhibitor, erlotinib, in Non-Small Cell Lung Cancer cell lines. Bioinformatic analysis of the signature showed a functional convergence on TGFβ canonical signalling. We hypothesized that TGFβ signalling controls expression of the miRNA genes comprising an erlotinib response signature in NSCLC. Western analysis revealed that TGFβ signalling via Smad2/3/4 occurred differently between erlotinib-resistant A549 and erlotinib- sensitive PC9 cells. We showed that TGFβ induced an interaction between Smad4 and putative Smad Binding Elements in PC9. However, qRT-PCR analysis showed that endogenous miR-140/141/200c expression changes resulted from time in treatments, not the treatments themselves. Moreover, flow cytometry indicated that cells exited the cell cycle in the same manner. Taken together these data indicated that the miRNA comprising the signature are likely regulated by the cell cycle rather than by TGFβ. Importantly, this work revealed that TGFβ did not induce EMT in PC9 cells, but rather TGFβ-inhibition induced an EMT-intermediate. These data also show that growth/proliferation signals by constitutively-activated EGFR may rely on TGFβ and a possible relationship between TGFβ and EGFR signalling may prevent EMT progression in this context rather than promote it.
Investigation of the removal mechanism of antibiotic ceftazidime by green algae and subsequent microbic impact assessment
Authors: Yu Ying , Zhou Yangyang , Wang Zhiliang , Torres Oscar Lopez , Guo Ruixin , Chen Jianqiu 
Published: June 2017

AbstractThe present study provides an integrated view of algal removal of the antibiotic ceftazidime and its basic parent structure 7-aminocephalosporanic acid (7-ACA), including contribution analysis, bacteriostatic and aquatic toxic assessment and metabolite verification. 92.70% and 96.07% of the two target compounds was removed after the algal treatment, respectively. The algal removal can be separated into three steps: a rapid adsorption, a slow cell wall-transmission and the final biodegradation. Additionally, while ceftazidime demonstrated an excellent inhibitory effect on Escherichia coli, there was no bacteriostasis introduced after the algal treatment, which could avoid favoring the harmful selective pressure. On the other hand, no significant aquatic impact of the two target compounds on rotifers was observed and it was not enhanced after the algal treatment. To better reveal the mechanism involved, metabolite analyses were performed. Δ-3 ceftazidime and trans-ceftazidime were regarded as the metabolites of ceftazidime and the metabolite of 7-ACA was regarded as a compound which shared the similar structure with 4-chlorocinnamic acid. Our study indicated that the green algae performed a satisfactory growth capacity and played a dominant role for the biodegradation of the target antibiotics, which achieved high removal efficiency and low environmental impact.
Characterization of a novel two-component Na+(Li+, K+)/H+ antiporter from Halomonas zhaodongensis
Authors: Meng Lin , Meng Fankui , Zhang Rui , Zhang Zhenglai , Dong Ping , Sun Kaifu , Chen Jin , Zhang Wei , Yan Mingxue , Li Jincheng , Abdel-motaal Heba , Jiang Juquan 
Published: June 2017

AbstractIn this study, genomic DNA was screened for novel Na+/H+ antiporter genes from Halomonas zhaodongensis by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters. Co-expression of two genes designated umpAB, encoding paired homologous unknown membrane proteins belonging to DUF1538 (domain of unknown function with No. 1538) family, were found to confer E. coli KNabc the tolerance to 0.4 M NaCl and 30 mM LiCl, and an alkaline pH resistance at 8.0. Western blot and co-immunoprecipitation establish that UmpAB localize as a hetero-dimer in the cytoplasmic membranes. Functional analysis reveals that UmpAB exhibit pH-dependent Na+(Li+, K+)/H+ antiport activity at a wide pH range of 6.5 to 9.5 with an optimal pH at 9.0. Neither UmpA nor UmpB showed homology with known single-gene or multi-gene Na+/H+ antiporters, or such proteins as ChaA, MdfA, TetA(L), Nap and PsmrAB with Na+/H+ antiport activity. Phylogenetic analysis confirms that UmpAB should belong to DUF1538 family, which are significantly distant with the above-mentioned proteins with Na+/H+ antiport activity. Taken together, we propose that UmpAB represent a novel two-component Na+(Li+, K+)/H+ antiporter. To the best of our knowledge, this is the first report on the functional analysis of unknown membrane proteins belonging to DUF1538 family.
Metabolic recovery of lipodystrophy, liver steatosis, and pancreatic β cell proliferation after the withdrawal of OSI-906
Authors: Tajima Kazuki , Shirakawa Jun , Togashi Yu , Yamazaki Shunsuke , Okuyama Tomoko , Kyohara Mayu , Konishi Hiromi , Terauchi Yasuo 
Published: June 2017

AbstractGrowth factor signaling via insulin receptor (IR) and IGF-1 receptor (IGF1R) plays several important roles in the pathogenesis of metabolic syndrome and diabetes. OSI-906 (linsitinib), an anti-tumor drug, is an orally bioavailable dual inhibitor of IR and IGF1R. To investigate the recovery from metabolic changes induced by the acute inhibition of IR and IGF1R in adult mice, mice were treated with OSI-906 or a vehicle for 7 days and the results were analyzed on the last day of injection (Day 7) or after 7 or 21 days of withdrawal (Day 14 or Day 28). On day 7, the visceral white fat mass was significantly reduced in mice treated with OSI-906 accompanied by a reduced expression of leptin and an increased expression of the lipolysis-related genes Lpl and Atgl. Interestingly, the lipoatrophy and the observed changes in gene expression were completely reversed on day 14. Similarly, liver steatosis and β cell proliferation were transiently observed on day 7 but had disappeared by day 14. Taken together, these results suggest that this model for the acute inhibition of systemic IR/IGF1R signaling may be useful for investigating the recovery from metabolic disorders induced by impaired growth factor signaling.