bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parallel expression profiling of hepatic and serum microRNA-122 associated with clinical features and treatment responses in chronic hepatitis C patients
Authors: Butt Azeem Mehmood , Raja Arsalan Jamil , Siddique Shafiqa , Khan Jahangir Sarwar , Shahid Muhammad , Tayyab Ghias-Un-Nabi , Minhas Zahid , Umar Muhammad , Idrees Muhammad , Tong Yigang 
Published: February 2016

AbstractMicroRNAs (miRNAs) are small, non-coding RNAs that regulate a variety of biological processes. Recently, human liver-specific miRNA miR-122 has been reported to facilitate hepatitis C virus (HCV) replication in liver cells. HCV is one of the leading causes of liver diseases worldwide. In Pakistan, the estimated prevalence is up to 10%. Here, we report hepatic and serum miR-122 expression profiling from paired liver and serum samples from treatment-naive chronic hepatitis C (CHC) patients and controls. We aimed to elucidate the biomarker potential of serum miR-122 for monitoring disease progression and predicting end treatment response (ETR). Hepatic miR-122 levels were significantly down-regulated in CHC patients. A significant inverse correlation was observed between hepatic and serum miR-122 levels, indicating that serum miR-122 levels reflect HCV-associated disease progression. Both hepatic and serum miR-122 were significantly correlated (P < 0.05) with several clinicopathological features of CHC. Receiver operator curve analysis showed that serum miR-122 had superior discriminatory ability even in patients with normal alanine transaminase levels. Multivariate logistic regression analysis highlighted pre-treatment serum miR-122 levels as independent predictors of ETR. In conclusion, serum miR-122 holds the potential to serve as a promising biomarker of disease progression and ETR in CHC patients.
Direct Optical Probing of Transverse Electric Mode in Graphene
Authors: Menabde Sergey G. , Mason Daniel R. , Kornev Evgeny E. , Lee Changhee , Park Namkyoo 
Published: February 2016

AbstractUnique electrodynamic response of graphene implies a manifestation of an unusual propagating and localised transverse-electric (TE) mode near the spectral onset of interband transitions. However, excitation and further detection of the TE mode supported by graphene is considered to be a challenge for it is extremely sensitive to excitation environment and phase matching condition adherence. Here for the first time, we experimentally prove an existence of the TE mode by its direct optical probing, demonstrating significant coupling to an incident wave in electrically doped multilayer graphene sheet at room temperature. We believe that proposed technique of careful phase matching and obtained access to graphene’s TE excitation would stimulate further studies of this unique phenomenon, and enable its potential employing in various fields of photonics as well as for characterization of graphene.
Ocean acidification increases copper toxicity differentially in two key marine invertebrates with distinct acid-base responses
Authors: Lewis Ceri , Ellis Robert P. , Vernon Emily , Elliot Katie , Newbatt Sam , Wilson Rod W. 
Published: February 2016

AbstractOcean acidification (OA) is expected to indirectly impact biota living in contaminated coastal environments by altering the bioavailability and potentially toxicity of many pH-sensitive metals. Here, we show that OA (pH 7.71; pCO2 1480 μatm) significantly increases the toxicity responses to a global coastal contaminant (copper ~0.1 μM) in two keystone benthic species; mussels (Mytilus edulis) and purple sea urchins (Paracentrotus lividus). Mussels showed an extracellular acidosis in response to OA and copper individually which was enhanced during combined exposure. In contrast, urchins maintained extracellular fluid pH under OA by accumulating bicarbonate but exhibited a slight alkalosis in response to copper either alone or with OA. Importantly, copper-induced damage to DNA and lipids was significantly greater under OA compared to control conditions (pH 8.14; pCO2 470 μatm) for both species. However, this increase in DNA-damage was four times lower in urchins than mussels, suggesting that internal acid-base regulation in urchins may substantially moderate the magnitude of this OA-induced copper toxicity effect. Thus, changes in metal toxicity under OA may not purely be driven by metal speciation in seawater and may be far more diverse than either single-stressor or single-species studies indicate. This has important implications for future environmental management strategies.
The modification of Gat1p in nitrogen catabolite repression to enhance non-preferred nitrogen utilization in Saccharomyces cerevisiae
Authors: Zhao Xinrui , Zou Huijun , Chen Jian , Du Guocheng , Zhou Jingwen 
Published: February 2016

AbstractInSaccharomyces cerevisiae, when preferred nitrogen sources are present, the metabolism of non-preferred nitrogen is repressed. Previous work showed that this metabolic regulation is primarily controlled by nitrogen catabolite repression (NCR) related regulators. Among these regulators, two positive regulators (Gln3p and Gat1p) could be phosphorylated and sequestered in the cytoplasm leading to the transcription of non-preferred nitrogen metabolic genes being repressed. The nuclear localization signals (NLSs) and nuclear localization regulatory signals (NLRSs) in Gln3p and Gat1p play essential roles in the regulation of their localization in cells. However, compared with Gln3p, the information of NLS and NLRS for Gat1p remains unknown. In this study, residues 348–375 and 366–510 were identified as the NLS and NLRS of Gat1p firstly. In addition, the modifications of Gat1p (mutations on the NLS and truncation on the NLRS) were attempted to enhance the transcription of non-preferred nitrogen metabolic genes. Quantitative real-time PCR showed that the transcriptional levels of 15 non-preferred nitrogen metabolic genes increased. Furthermore, during the shaking-flask culture tests, the utilization of urea, proline and allantoine was significantly increased. Based on these results, the genetic engineering on Gat1p has a great potential in enhancing non-preferred nitrogen metabolism inS. cerevisiae.
Biochemical characterization of extra- and intracellular endoxylanse from thermophilic bacterium Caldicellulosiruptor kronotskyensis
Authors: Jia Xiaojing , Qiao Weibo , Tian Wenli , Peng Xiaowei , Mi Shuofu , Su Hong , Han Yejun 
Published: February 2016

AbstractCaldicellulosiruptor kronotskyensis grows on lignocellulosic biomass by the catalysis of intrinsic glycoside hydrolase and has potential application for consolidated bioprocessing. In current study, two predicted extra- (Xyn10A) and intracellular (Xyn10B) xylanase from C. kronotskyensis were comparatively characterized. Xyn10A and Xyn10B share GH10 catalytic domain with similarity of 41%, while the former contains two tandem N-terminus CBM22s. Xyn10A showed higher hydrolytic capability than Xyn10B on both beechwood xylan (BWX) and oat spelt xylan (OSX). Truncation mutation experiments revealed the importance of CBMs for hydrolytic activity, substrate binding and thermostability of Xyn10A.While the quantity of CBM was not directly related to bind and thermostability. Although CBM was considered to be crucial for substrate binding, Xyn10B and Xyn10A as well as truncations performed similar binding affinity to insoluble substrate OSX. Analysis of point mutation revealed similar key residues, Glu493, Glu601 and Trp658 for Xyn10A and Glu139, Glu247 and Trp305 for Xyn10B. Both Xyn10A and Xyn10B exhibited hydrolytic activity on the mechanical pretreated corncob. After pre-digested by Xyn10A or Xyn10B, the micropores inthe the mechanical pretreated corncob were observed, which enhanced the accessibility for cellulase. Compared with corncob hydrolyzed with cellulase alone, enhanced hydrolytic performance of was observed after pre-digestion by Xyn10A or Xyn10B.
Integration of conductive reduced graphene oxide into microstructured optical fibres for optoelectronics applications
Authors: Ruan Yinlan , Ding Liyun , Duan Jingjing , Ebendorff-Heidepriem Heike , Monro Tanya M. 
Published: February 2016

AbstractIntegration of conductive materials into optical fibres can largely expand functions of fibre devices including surface plasmon resonator/metamaterial, modulators/detectors, or biosensors. Some early attempts have been made to incorporate metals such as tin into fibres during the fibre drawing process. Due to the restricted range of materials that have compatible melting temperatures with that of silica glass, the methods to incorporate metals along the length of the fibres are very challenging. Moreover, metals are nontransparent with strong light absorption, which causes high fibre loss. This article demonstrates a novel but simple method for creating transparent conductive reduced graphene oxide film onto microstructured silica fibres for potential optoelectronic applications. The strongly confined evanescent field of the suspended core fibres with only 2 μW average power was creatively used to transform graphene oxide into reduced graphene oxide with negligible additional loss. Existence of reduced graphene oxide was confirmed by their characteristic Raman signals, shifting of their fluorescence peaks as well as largely decreased resistance of the bulk GO film after laser beam exposure.
On the causal structure between CO2 and global temperature
Authors: Stips Adolf , Macias Diego , Coughlan Clare , Garcia-Gorriz Elisa , Liang X. San 
Published: February 2016

AbstractWe use a newly developed technique that is based on the information flow concept to investigate the causal structure between the global radiative forcing and the annual global mean surface temperature anomalies (GMTA) since 1850. Our study unambiguously shows one-way causality between the total Greenhouse Gases and GMTA. Specifically, it is confirmed that the former, especially CO2, are the main causal drivers of the recent warming. A significant but smaller information flow comes from aerosol direct and indirect forcing and on short time periods, volcanic forcings. In contrast the causality contribution from natural forcings (solar irradiance and volcanic forcing) to the long term trend is not significant. The spatial explicit analysis reveals that the anthropogenic forcing fingerprint is significantly regionally varying in both hemispheres. On paleoclimate time scales, however, the cause-effect direction is reversed: temperature changes cause subsequent CO2/CH4 changes.
The role of nano-perovskite in the negligible thorium release in seawater from Greek bauxite residue (red mud)
Authors: Gamaletsos Platon N. , Godelitsas Athanasios , Kasama Takeshi , Kuzmin Alexei , Lagos Markus , Mertzimekis Theo J. , Göttlicher Jörg , Steininger Ralph , Xanthos Stelios , Pontikes Yiannis , Angelopoulos George N. , Zarkadas Charalampos , Komelkov Aleksandr , Tzamos Evangelos , Filippidis Anestis 
Published: February 2016

AbstractWe present new data about the chemical and structural characteristics of bauxite residue (BR) from Greek Al industry, using a combination of microscopic, analytical, and spectroscopic techniques. SEM-EDS indicated a homogeneous dominant “Al-Fe-Ca-Ti-Si-Na-Cr matrix”, appearing at the microscale. The bulk chemical analyses showed considerable levels of Th (111 μg g−1), along with minor U (15 μg g−1), which are responsible for radioactivity (355 and 133 Bq kg−1 for 232Th and 238U, respectively) with a total dose rate of 295 nGy h−1. Leaching experiments, in conjunction with SF-ICP-MS, using Mediterranean seawater from Greece, indicated significant release of V, depending on S/L ratio, and negligible release of Th at least after 12 months leaching. STEM-EDS/EELS & HR-STEM-HAADF study of the leached BR at the nanoscale revealed that the significant immobility of Th4+ is due to its incorporation into an insoluble perovskite-type phase with major composition of Ca0.8Na0.2TiO3 and crystallites observed in nanoscale. The Th LIII-edge EXAFS spectra demonstrated that Th4+ ions, which are hosted in this novel nano-perovskite of BR, occupy Ca2+ sites, rather than Ti4+ sites. That is most likely the reason of no Th release in Mediterranean seawater.
No rapid audiovisual recalibration in adults on the autism spectrum
Authors: Turi Marco , Karaminis Themelis , Pellicano Elizabeth , Burr David 
Published: February 2016

AbstractAutism spectrum disorders (ASD) are characterized by difficulties in social cognition, but are also associated with atypicalities in sensory and perceptual processing. Several groups have reported that autistic individuals show reduced integration of socially relevant audiovisual signals, which may contribute to the higher-order social and cognitive difficulties observed in autism. Here we use a newly devised technique to study instantaneous adaptation to audiovisual asynchrony in autism. Autistic and typical participants were presented with sequences of brief visual and auditory stimuli, varying in asynchrony over a wide range, from 512 ms auditory-lead to 512 ms auditory-lag and judged whether they seemed to be synchronous. Typical adults showed strong adaptation effects, with trials proceeded by an auditory-lead needing more auditory-lead to seem simultaneous and vice versa. However, autistic observers showed little or no adaptation, although their simultaneity curves were as narrow as the typical adults. This result supports recent Bayesian models that predict reduced adaptation effects in autism. As rapid audiovisual recalibration may be fundamental for the optimisation of speech comprehension, recalibration problems could render language processing more difficult in autistic individuals, hindering social communication.
Activated FXR Inhibits Leptin Signaling and Counteracts Tumor-promoting Activities of Cancer-Associated Fibroblasts in Breast Malignancy
Authors: Giordano Cinzia , Barone Ines , Vircillo Valentina , Panza Salvatore , Malivindi Rocco , Gelsomino Luca , Pellegrino Michele , Rago Vittoria , Mauro Loredana , Lanzino Marilena , Panno Maria Luisa , Bonofiglio Daniela , Catalano Stefania , Andò Sebastiano 
Published: February 2016

AbstractCancer-associated fibroblasts (CAFs), the principal components of the tumor stroma, play a central role in cancer development and progression. As an important regulator of the crosstalk between breast cancer cells and CAFs, the cytokine leptin has been associated to breast carcinogenesis. The nuclear Farnesoid X Receptor-(FXR) seems to exert an oncosuppressive role in different tumors, including breast cancer. Herein, we demonstrated, for the first time, that the synthetic FXR agonist GW4064, inhibiting leptin signaling, affects the tumor-promoting activities of CAFs in breast malignancy. GW4064 inhibited growth, motility and invasiveness induced by leptin as well as by CAF-conditioned media in different breast cancer cell lines. These effects rely on the ability of activated FXR to increase the expression of the suppressor of the cytokine signaling 3 (SOCS3) leading to inhibition of leptin-activated signaling and downregulation of leptin-target genes. In vivo xenograft studies, using MCF-7 cells alone or co-injected with CAFs, showed that GW4064 administration markedly reduced tumor growth. Interestingly, GW4064-treated tumors exhibited decreased levels of leptin-regulated proteins along with a strong staining intensity for SOCS3. Thus, FXR ligands might represent an emerging potential anti-cancer therapy able to block the tumor supportive role of activated fibroblasts within the breast microenvironment.
CD36 is a co-receptor for hepatitis C virus E1 protein attachment
Authors: Cheng Jun-Jun , Li Jian-Rui , Huang Meng-Hao , Ma Lin-Lin , Wu Zhou-Yi , Jiang Chen-Chen , Li Wen-Jing , Li Yu-Huan , Han Yan-Xing , Li Hu , Chen Jin-Hua , Wang Yan-Xiang , Song Dan-Qing , Peng Zong-Gen , Jiang Jian-Dong 
Published: February 2016

AbstractThe cluster of differentiation 36 (CD36) is a membrane protein related to lipid metabolism. We show that HCV infection in vitro increased CD36 expression in either surface or soluble form. HCV attachment was facilitated through a direct interaction between CD36 and HCV E1 protein, causing enhanced entry and replication. The HCV co-receptor effect of CD36 was independent of that of SR-BI. CD36 monoclonal antibodies neutralized the effect of CD36 and reduced HCV replication. CD36 inhibitor sulfo-N-succinimidyl oleate (SSO), which directly bound CD36 but not SR-BI, significantly interrupted HCV entry, and therefore inhibited HCV replication. SSO’s antiviral effect was seen only in HCV but not in other viruses. SSO in combination with known anti-HCV drugs showed additional inhibition against HCV. SSO was considerably safe in mice. Conclusively, CD36 interacts with HCV E1 and might be a co-receptor specific for HCV entry; thus, CD36 could be a potential drug target against HCV.
Differentially expressed lncRNAs and mRNAs identified by microarray analysis in GBS patients vs healthy controls
Authors: Xu Jing , Gao Chao , Zhang Fang , Ma Xiaofeng , Peng Xiaolin , Zhang Rongxin , Kong Dexin , Simard Alain R. , Hao Junwei 
Published: February 2016

AbstractThe aim of our present study was to determine whether message RNAs (mRNAs) and long noncoding RNAs (lncRNAs) are expressed differentially in patients with Guillain-Barré syndrome (GBS) compared with healthy controls. The mRNA and lncRNA profiles of GBS patients and healthy controls were generated by using microarray analysis. From microarray analysis, we listed 310 mRNAs and 114 lncRNAs with the mRMR software classed into two sample groups, GBS patients and healthy controls. KEGG mapping demonstrated that the top seven signal pathways may play important roles in GBS development. Several GO terms, such as cytosol, cellular macromolecular complex assembly, cell cycle, ligase activity, protein catabolic process, etc., were enriched in gene lists, suggesting a potential correlation with GBS development. Co-expression network analysis indicated that 113 lncRNAs and 303 mRNAs were included in the co-expression network. Our present study showed that these differentially expressed mRNAs and lncRNAs may play important roles in GBS development, which provides basic information for defining the mechanism(s) that promote GBS.
Graphene oxide-dependent growth and self-aggregation into a hydrogel complex of exoelectrogenic bacteria
Authors: Yoshida Naoko , Miyata Yasushi , Doi Kasumi , Goto Yuko , Nagao Yuji , Tero Ryugo , Hiraishi Akira 
Published: February 2016

AbstractGraphene oxide (GO) is reduced by certain exoelectrogenic bacteria, but its effects on bacterial growth and metabolism are a controversial issue. This study aimed to determine whether GO functions as the terminal electron acceptor to allow specific growth of and electricity production by exoelectrogenic bacteria. Cultivation of environmental samples with GO and acetate as the sole substrate could specifically enrich exoelectrogenic bacteria withGeobacterspecies predominating (51–68% of the total populations). Interestingly, bacteria in these cultures self-aggregated into a conductive hydrogel complex together with biologically reduced GO (rGO). A novel GO-respiring bacterium designatedGeobactersp. strain R4 was isolated from this hydrogel complex. This organism exhibited stable electricity production at >1000 μA/cm3(at 200 mV vs Ag/AgCl) for more than 60 d via rGO while temporary electricity production using graphite felt. The better electricity production depends upon the characteristics of rGO such as a large surface area for biofilm growth, greater capacitance and smaller internal resistance. This is the first report to demonstrate GO-dependent growth of exoelectrogenic bacteria while forming a conductive hydrogel complex with rGO. The simple put-and-wait process leading to the formation of hydrogel complexes of rGO and exoelectrogens will enable wider applications of GO to bioelectrochemical systems.
RETRACTED ARTICLE: HMGB1 Facilitated Macrophage Reprogramming towards a Proinflammatory M1-like Phenotype in Experimental Autoimmune Myocarditis Development
Authors: Su Zhaoliang , Zhang Pan , Yu Ying , Lu Hongxiang , Liu Yanfang , Ni Ping , Su Xiaolian , Wang Dan , Liu Yueqin , Wang Jia , Shen Huiling , Xu Wenlin , Xu Huaxi 
Published: February 2016

AbstractMacrophages can be reprogramming, such as the classical activated macrophage, M1 or alternative activated macrophages, M2 phenotype following the milieu danger signals, especially inflammatory factors. Macrophage reprogramming is now considered as a key determinant of disease development and/or regression. Experimental autoimmune myocarditis (EAM) is characterized by monocytes/macrophage infiltration, Th17 cells activation and inflammatory factors producing such as high mobility group box 1 (HMGB1). Whether infiltrated macrophages could be reprogramming in EAM? HMGB1 was associated with macrophage reprogramming? Our results clearly demonstrated that infiltrated macrophage was reprogrammed towards a proinflammatory M1-like phenotype and cardiac protection by monocytes/macrophages depletion or HMGB1 blockade in EAM; in vitro, HMGB1 facilitated macrophage reprogramming towards M1-like phenotype dependent on TLR4-PI3Kγ-Erk1/2 pathway; furthermore, the reprogramming M1-like macrophage promoted Th17 expansion. Therefore, we speculated that HMGB1 contributed EAM development via facilitating macrophage reprogramming towards M1-like phenotype except for directly modulating Th17 cells expansion.
Differential Odour Coding of Isotopomers in the Honeybee Brain
Authors: Paoli Marco , Anesi Andrea , Antolini Renzo , Guella Graziano , Vallortigara Giorgio , Haase Albrecht 
Published: February 2016

AbstractThe shape recognition model of olfaction maintains that odorant reception probes physicochemical properties such as size, shape, electric charge and hydrophobicity of the ligand. Recently, insects were shown to distinguish common from deuterated isotopomers of the same odorant, suggesting the involvement of other molecular properties to odorant reception. Via two-photon functional microscopy we investigated how common and deuterated isoforms of natural odorants are coded within the honeybee brain. Our results provide evidence that (i) different isotopomers generate different neuronal activation maps, (ii) isotopomer sensitivity is a general mechanism common to multiple odorant receptors and (iii) isotopomer specificity is highly consistent across individuals. This indicates that honeybee’s olfactory system discriminates between isotopomers of the same odorant, suggesting that other features, such as molecular vibrations, may contribute to odour signal transduction.
A combination of serum iron, ferritin and transferrin predicts outcome in patients with intracerebral hemorrhage
Authors: Yang Guang , Hu Rong , Zhang Chao , Qian Christopher , Luo Qian-Qian , Yung Wing-Ho , Ke Ya , Feng Hua , Qian Zhong-Ming 
Published: February 2016

AbstractAssociation of a high-serum ferritin with poor outcome showed that iron might play a detrimental role in the brain after intracerebral hemorrhage (ICH). Here, we investigated changes in serum iron, ferritin, transferrin (Tf) and ceruloplasmin (CP) in patients with ICH (n = 100) at day 1 (admission), 3, 7, 14 and 21 and those in control subjects (n = 75). The hematoma and edema volumes were also determined in ICH-patients on admission and at day 3. The Modified Rankin Scale (mRS) of 59 patients was ≥3 (poor outcome) and 41 < 3 (good outcome) at day 90. Serum ferritin was significantly higher and serum iron and Tf markedly lower in patients with poor-outcome than the corresponding values in patients with good-outcome at day 1 to 7 and those in the controls. There was a significant positive correlation between serum ferritin and relative edema volume or ratio at day 1 and 3 and hematoma volume at day 1 (n = 28), and a negative correlation between serum iron or Tf and hematoma volume at day 1 (n = 100). We concluded that not only increased serum ferritin but also reduced serum iron and Tf are associated with outcome as well as hematoma volume.