bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-color fluorescence live-cell imaging inDictyostelium discoideum
Authors: Hashimura Hidenori , Kuwana Satoshi , Nakagwa Hibiki , Abe Kenichi , Adachi Tomoko , Sugita Toyoko , Fujishiro Shoko , Honda Gen , Sawai Satoshi 
Published: October 2024

AbstractThe cellular slime moldDictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology.D. discoideumis unique in that they are genetically tractable, with a wealth of data accumulated over half a century of research. Fluorescence live-cell imaging ofD. discoideumhas greatly facilitated studies on fundamental topics, including cytokinesis, phagocytosis, and cell migration. Additionally, its unique life cycle placesDictyosteliumat the forefront of understanding aggregative multicellularity, a recurring evolutionary trait found across the Opisthokonta and Amoebozoa clades. The use of multiple fluorescent proteins (FP) and labels with separable spectral properties is critical for tracking cells in aggregates and identifying co-occurring biomolecular events and factors that underlie the dynamics of the cytoskeleton, membrane lipids, second messengers, and gene expression. However, inD. discoideum, the number of frequently used FP species is limited to two or three. In this study, we explored the use of new-generation FP for practical 4- to 5-color fluorescence imaging ofD. discoideum. We showed that the yellow fluorescent protein Achilles and the red fluorescent protein mScarlet-I both yield high signals and allow sensitive detection of rapid gene induction. The color palette was further expanded to include blue (mTagBFP2 and mTurquosie2), large Stoke-shift LSSmGFP, and near-infrared (miRFP670nano3) FPs, in addition to the HaloTag ligand SaraFluor 650T. Thus, we demonstrated the feasibility of deploying 4- and 5- color imaging ofD. discoideumusing conventional confocal microscopy.
A Transcriptional Signature of Induced Neurons Differentiates Virologically Suppressed People Living With HIV from People Without HIV
Authors: Ostermann Philipp N. , Wu Youjun , Bowler Scott A. , Siddiqui Mohammad Adnan , Herrera Alberto , Sidharta Mega , Ramnarine Kiran , Martínez-Meza Samuel , St. Bernard Leslie Ann , Nixon Douglas F. , Jones R. Brad , Yamashita Masahiro , Ndhlovu Lishomwa C. , Zhou Ting , Evering Teresa H. 
Published: October 2024

AbstractNeurocognitive impairment is a prevalent and important co-morbidity in virologically suppressed people living with HIV (PLWH), yet the underlying mechanisms remain elusive and treatments lacking. Here, we explored for the first time, use of participant-derived directly induced neurons (iNs) to model neuronal biology and injury in PLWH. iNs retain age-and disease-related features of the donors, providing unique opportunities to reveal novel aspects of neurological disorders. We obtained primary dermal fibroblasts from six virologically suppressed PLWH (range: 27 – 64 years, median: 53); 83% Male; 50% White) and seven matched people without HIV (PWOH) (range: 27 – 66, median: 55); 71% Male; 57% White). iNs were generated using transcription factors NGN2 and ASCL1, and validated by immunocytochemistry and single-cell-RNAseq. Transcriptomic analysis using bulk-RNAseq identified 29 significantly differentially expressed genes between iNs from PLWH and PWOH. Of these, 16 genes were downregulated and 13 upregulated in PLWH iNs. Protein-protein interaction network mapping indicates that iNs from PLWH exhibit differences in extracellular matrix organization and synaptic transmission.IFI27was upregulated in iNs from PLWH, which complements independent post-mortem studies demonstrating elevatedIFI27expression in PLWH-derived brain tissue, indicating that iN generation reconstitutes this pathway. Finally, we observed that expression of theFOXL2NB-FOXL2-LINC01391genome locus is reduced in iNs from PLWH and negatively correlates with neurocognitive impairment. Thus, we have identified an iN gene signature of HIV through direct reprogramming of skin fibroblasts into neurons revealing novel mechanisms of neurocognitive impairment in PLWH.One sentence summaryDirect reprogramming of skin fibroblasts into neurons reveals unique gene signatures indicative of HIV infection in the context of viral suppression.
Environmental stability of HPAIV H5N1 in raw milk, wastewater and on surfaces
Authors: Kaiser Franziska , Cardenas Santiago , Yinda Kwe Claude , Mukesh Reshma K. , Ochwoto Missiani , Gallogly Shane , Wickenhagen Arthur , Bibby Kyle , de Wit Emmie , Morris Dylan , Lloyd-Smith James O. , Munster Vincent J. 
Published: October 2024

AbstractH5N1 influenza outbreaks in dairy cows necessitate studying potential transmission routes among livestock and to humans. We measured the stability of infectious H5N1 influenza virus in raw milk, wastewater, and on contaminated surfaces. We found relatively slow decay in milk, indicating that contaminated milk and fomites pose plausible transmission risks.
Growing Macrophages Regulate High Rates of Solute Flux by Pinocytosis
Authors: Tebeje Biniam M. , Thiex Natalie W. , Swanson Joel A. 
Published: October 2024

AbstractIn metazoan cells, growth factors stimulate solute ingestion by pinocytosis. To examine the role of pinocytosis in cell growth, this study measured cell proliferation and the attendant rates of solute flux by pinocytosis in murine macrophages in response to the growth factor colony-stimulating factor-1 (CSF1). During CSF1-dependent growth in rich medium, macrophages internalized 72 percent of their cell volume in extracellular fluid every hour. Removal of the essential amino acid leucine from growth medium limited rates of protein synthesis and growth, but increased rates of solute accumulation by macropinocytosis. The amount of protein synthesized during leucine-dependent growth exceeded the capacity of pinocytosis to internalize enough soluble leucine to support growth and proliferation. Fluid-phase solute recycling from lysosomes secreted small molecules from the cells at high rates. Inhibitors of pinocytosis and the mechanistic target-of-rapamycin (mTOR) reduced cell growth and solute recycling, indicating roles for pinocytosis in growth and for nutrient sensing in the regulation of solute flux by pinocytosis.SummaryMurine macrophages growing in response to colony-stimulating factor-1 (CSF1) require pinocytosis. High rates of solute influx and accumulation by pinocytosis are regulated by CSF1 and leucine. Low molecular weight products of protein hydrolysis in lysosomes recycle efficiently from the cells.
Women entrepreneurship: investigating the influence of emotional intelligence and innovativeness on entrepreneurial success
Authors: Kovid Raj K. , Singh Neetika , Sharma Gunjan Mohan , Gaur Priya , Pandla Kapil 
Published: October 2024

PurposeThe study is designed to investigate whether women’s entrepreneurial success is affected by their emotional intelligence. It also investigates the mediating role of innovativeness in influencing the relationship of their emotional intelligence with entrepreneurial success.Design/methodology/approachUsing a cross-sectional design, this study used a structured questionnaire to gather data from 274 women entrepreneurs spanning across various industries in north India. The data collected was analysed using the partial least squares structured equation modelling (PLS-SEM) technique.FindingsThe findings demonstrate a notable and positive association between emotional intelligence and the entrepreneurial success of women. Additionally, innovativeness was found to partially mediate the relationship between emotional intelligence and entrepreneurial success. Entrepreneurial experience moderates the relationship between emotional intelligence and entrepreneurial success. Furthermore, age was not observed to moderate the connection between emotional intelligence and entrepreneurial success.Originality/valueThis study provides valuable insights for female entrepreneurs seeking to achieve success by harnessing their emotional intelligence and fostering innovativeness in their entrepreneurial pursuits. The study mainly provides evidence on the conclusive role of emotional intelligence among women entrepreneurs in their success in the relatively underresearched context of India, thereby consolidating knowledge on gendered entrepreneurship.
Forecasting exchange rates after 2021 Myanmar coup: Does a parallel market exchange rate matter?
Authors: Panthamit Nisit , Chaiboonsri Chukiet , Boonyasana Pimonpun , Bureecam Chira , Tian Guowei 
Published: October 2024

PurposeThe purpose of this study is to evaluate the use of a parallel market mechanism in forecasting the exchange rate of Myanmar Kyat (MMK) after the 2021 Myanmar coup. This paper seeks to answer how an official fixed exchange rate is influenced by a parallel market rate during the military coup. This study demonstrates both the benefit of understanding the impact of a market mechanism and the unique value of leveraging deep learning (DL).Design/methodology/approachThis study applies DL for the first time to forecast the daily official exchange rates and parallel exchange rates, covering the period January 5, 2021–August 22, 2022. This study shows the insights available from forecasting the market exchange rate of MMK in parallel markets that are sensitive to political changes for Myanmar.FindingsAfter the February 2021 military coup in Myanmar, the subsequent use of an untrusted exchange rate system in Myanmar resulted in a sharp gap between the official and the parallel exchange rates. The study shows that the actual forecasting of exchange rates is primarily a reflection of an active market mechanism rather than official exchange rates set by the monetary authorities.Originality/valueNo previous study has forecast the exchange rate of Myanmar since the latest Myanmar coup in 2021. This study contributes crucial predictions, provides a robust technique “deep learning”.
Are acute hospital trust mergers associated with improvements in the quality of care?
Authors: Beveridge James , Lugo-Palacios David G. , Clarke Jonathan 
Published: October 2024

PurposeThis study aims to assess the extent to which acute hospital trust mergers in England are associated with quality improvements.Design/methodology/approachWe apply an event study design using difference-in-difference (DID) and coarsened exact matching to compare the before-and-after performance of eight mergers from 2011 to 2015.FindingsWe find little evidence that mergers contribute to quality improvements other than some limited increases in the proportion of patients waiting a maximum of 18 weeks from referral to treatment. We postulate that financial incentives and political influence could have biased management effort towards waiting time measures.Research limitations/implicationsInherent sample size constraints may limit generalisability. Merger costs and complexity mean they are unlikely to offer an efficient strategy for helping to clear elective care backlogs. We recommend further research into causal mechanisms to help health systems maximise benefits from both mergers and emerging models of hospital provider collaboration.Originality/valueThis paper is the first to study the quality impact of a new wave of acute hospital mergers taking place in the English National Health Service from 2011 onwards, applying a group-time DID estimator to account for multiple treatment timings.
Trade-off between knowledge-dynamic mechanisms in linking perceived injustice with organizational performance: moderation function of job experience
Authors: Selem Kareem M. , Shoukat Muhammad Haroon 
Published: October 2024

PurposeThis paper, underpinned by knowledge dynamics theory (KDT), seeks to investigate the pivotal role of knowledge translation mechanisms such as knowledge hiding (KH) and knowledge sharing (KS) in the nexus between perceived organizational injustice (POI) and organizational performance (OP). Furthermore, we examined the moderating effect of job experiences on the nexuses between KH-OP and KS-OP.Design/methodology/approachData were collected from 289 hospital professionals at 23 private hospitals in Pakistan using a time-lagged approach. PLS-SEM was run to test hypotheses.FindingsKH and KS significantly established the POI-OP linkage, while employee job experience improved the KH-OP and KS-OP relationships.Practical implicationsFindings suggest that policymakers should develop policies for private hospital employees to reduce perceived injustice by emphasizing equal treatment and transparency in hospitals. Well-formulated policies help reduce perceived injustice, likely encouraging knowledge translation among hospital employees, resulting in increased OP.Originality/valueDrawing on KDT, this paper provides a novel approach for testing knowledge translation mechanisms between POI and OP relationships through pivotal roles of KH and KS and job experiences.
The impact of visionary leadership on product quality from the perspective of quality assurance managers in small and medium-sized companies: the mediating role of continual improvement
Authors: Alkhawaldeh Khleef 
Published: October 2024

PurposeA review of relevant literature supported the need to examine the nature of the relationships (if any) between visionary leadership, continual improvement and product quality. This study examined the relationship between visionary leadership and product quality and investigated the mediating role of continual improvement in the relationship between visionary leadership and product quality.Design/methodology/approachVisionary leadership, continual improvement and product quality were measured through a questionnaire developed based on a theoretical framework derived from the literature and distributed to quality assurance managers in small and medium-sized companies in the engineering, electrical and information technology sectors in Jordan.FindingsThe results indicated that the levels of visionary leadership, continual improvement and product quality are good and more from the perspective of quality assurance managers in these companies. The results also indicated that there is a positive, statistically significant relationship between visionary leadership and continual improvement and between continual improvement and product quality. Moreover, the results indicated that the relationship between visionary leadership and product quality is partially mediated by continual improvement. This indicates that there is a direct, positive and statistically significant relationship between visionary leadership and product quality.Originality/valueTo the author’s knowledge, this is the first study that examined the relationship between visionary leadership, continual improvement and product quality and investigated the mediating role of continual improvement in the relationship between visionary leadership and product quality. This study concluded that visionary leadership is the driver of all improvement initiatives and activities, which in turn improve the final products. The study contributes to the literature by revealing that visionary leadership leads to continual improvement and thus product quality, supporting the sequential relationship between them. Accordingly, the study provides useful theoretical insights and practical implications.
Hypertension and Cognitive Dysfunction in a Pregnant Rat Model of PE; a Role for CD4+ T Cells
Authors: Deer Evangeline , Herrock Owen , Simmons Kimberly , Campbell Nathan , Amaral Lorena , Zheng Baoying , Morris Rachael , Wallace Kedra , Cleveland ELizabeth Hawthorne , Belk Sheila , Dodd Cameronne , LaMarca Babbette 
Published: October 2024

ABSTRACTObjectivePreeclampsia (PE) is associated with hypertension (HTN) during pregnancy and activated CD4+ T cells, inflammatory cytokines, and autoantibodies to the angiotensin II type I receptor (AT1‐AA). Having had COVID‐19 (CV) during pregnancy is associated with an increased incidence of a PE‐like phenotype. Both PE and CV have long‐lasting neurological implications and studies show that nonpregnant COVID patients produce AT1‐AA. We have shown that CD4+ T cells from PE women cause a PE phenotype in nude athymic rats. In this study, we sought to examine the role of CD4+ T cells from PE with a CV History (Hx) to contribute to a PE phenotype and to determine the importance of CD4+ T cells in cognitive dysfunction during pregnancy.MethodsAt delivery, blood and placentas were collected, and one million placental CD4+ T cells from each PE and each normotensive patient, with (NT) or without (NP) a CV (Hx) during pregnancy, were isolated, purified, and injected i.p. into a gestational day (GD) 12 pregnant nude athymic rat (one patient/rat). At GD19, blood pressure (MAP) and circulating factors were assessed in recipient rats. Cognitive function and memory were assessed using Novel Object Recognition and Barnes Maze tests, respectively. Placental ACE‐2 activity and AT1‐AA were measured from COVID Hx patients. A one‐ or two‐way ANOVA with Bonferroni's multiple comparisons test was used for statistical analysis.ResultsBlood pressure was increased in patients with PE, with or without COVID, compared to NT patients. There were no significant changes in placental ACE activity in patients with COVID Hx with or without PE. AT1‐AA was elevated in PE patients and in both PE and NT COVID Hx compared to control NP. In pregnant recipient rats, MAP increased in CV Hx PE (113 ± 2, n = 8) compared to CV Hx NT (101 ± 5, n = 6). PE and PE CV Hx CD4+ T Cell recipient rats exhibited impaired memory and cognitive dysfunction (p < 0.05), compared to control groups. Recipient rats of PE CV Hx CD4+ T cells had elevated AT1‐AA compared to NT CV Hx recipients. Both COVID Hx groups and recipients of PE CD4+ T cells had elevated TNF alpha compared to NP.ConclusionOur findings indicate that pregnant patients with a Hx of COVID during pregnancy produce AT1‐AA, with or without PE. Recipients of CD4+ T cells from PE with or without a CV Hx during pregnancy cause HTN and elevated AT1‐AA. TNF‐α is elevated in PE and in CV Hx NT and PE recipients. Interestingly, recipients of T cells from PE patients with or without a Hx of CV had worse cognitive function during pregnancy, compared to recipient rats of NP CD4+ T cells. These data demonstrate the importance of CD4+ T cells in HTN and impaired neurological function during PE in the presence or absence of a prior COVID‐19 infection during pregnancy.
Victims of the Itaewon crowd crush died twice: The role of justice beliefs (general vs. personal) and fatalism in predicting victim‐blaming
Authors: Choi Hoon‐Seok , Seo Jeong‐Gil 
Published: October 2024

AbstractThe current study analyzes how the two facets of justice beliefs (GBJW and PBJW) relate to victim‐blaming in a recent man‐made disaster that occurred during the Halloween Festival in Seoul, South Korea. We also explore the psychological mechanism that underlies the link between justice beliefs and victim‐blaming by analyzing the mediating role of fatalism. We conducted a two‐wave survey over the first 10 days of the disaster (N = 185 and 154 for Time 1 and Time 2, respectively). We found that the GBJW positively predicted victim‐blaming at both Time 1 and Time 2, and this relationship was mediated by fatalistic cognitions about life. By contrast, the PBJW was not related to victim‐blaming across the two measurement points. We discuss the theoretical and practical implications of the findings and directions for future research on the link between justice beliefs and victim‐blaming.
Immunoglobin attenuates fulminant myocarditis by inhibiting overactivated innate immune response
Authors: Wen Jianpei , Li Huihui , Zhou Yufei , Du Hengzhi , Hu Guo , Wen Zheng , Tang Du , Wang Yanwen , Cui Xinwu , Zhou Zhou , Wang Dao Wen , Chen Chen 
Published: October 2024

Background and PurposeFulminant myocarditis (FM) is a myocardial inflammatory disease that can result from either viral diseases or autoimmune diseases. In this study, we have determined the treatment effects of immunomodulatory drugs on FM.Experimental ApproachFM was induced in A/JGpt mice by intraperitoneal administration of coxsackievirus B3, after which immunoglobins were administered daily by intraperitoneal injection. On the seventh day, the cardiac structure and function were determined using echocardiography and cardiac catheterisation. Single‐cell RNA sequencing (scRNA‐seq) was performed to evaluate CD45+ cells in the heart.Key ResultsImmunoglobin, a typical immunomodulatory drug, dramatically reduced mortality and significantly improved cardiac function in mice with FM. ScRNA‐seq revealed that immunoglobin treatment effectively modulated cardiac immune homeostasis, particularly by attenuating overactivated innate immune responses. At the cellular level, immunoglobin predominantly targeted Plac8+ monocytes and S100a8+ neutrophils, suppressing their proinflammatory activities, and enhancing antigen processing and presentation capabilities, thereby amplifying the efficiency and potency of the immune response against the virus. Immunoglobin benefits are mediated by the modulation of multiple signalling pathways, including relevant receptors on immune cells, direction of inflammatory cell chemotaxis, antigen presentation and anti‐viral effects. Subsequently, Bst2-ILT7 ligand‐receptor‐mediated cellular interactions manipulated by immunoglobin were further confirmed in vivo.Conclusions and ImplicationsImmunoglobin treatment significantly attenuated FM‐induced cardiac inflammation and improved cardiac function by inhibiting overactivated innate immune responses.
Between advocacy and commodification on Instagram: A case study of sharenting a child with disabilities
Authors: Ruiz‐Gomez Alexandra , Marôpo Lidia , Jorge Ana 
Published: October 2024

AbstractThis study examines the advocacy for children with disabilities in the context of sharenting practices on social media. The focus is on a case study of a 5‐year‐old British girl with Down syndrome (DS) named AC, whose Instagram account is managed by her mother. The study involved analysing all the content shared on the account, including single images, carousels, and videos (n = 1195), Reels (n = 310), pinned highlights (n = 6), and content tagged by other accounts (n = 74). The Instagram narratives were inductively categorized into three groups: Normalizing DS, Coping with DS, and DS macro‐level advocacy. The sharenting portrayal of AC has contributed to the growth of a supportive community challenging social stereotypes while promoting a more inclusive society. However, the study raises concerns about privacy, commodification, and self‐representation of the child in the context of sharenting advocacy. The mother's efforts to blend advocacy with commodification may compromise AC's privacy in pursuit of public recognition of DS children and financial benefits. While the social media portrayal attempts to offer the child's perspective, this case may not accurately represent self‐advocacy.