bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of the Abundance of Mercury from the Hg ii Line at 5677.10 Å in Late B-type Stars. VI. HD 173524 (46 Dra)
Authors: Monier Richard 
Published: September 2024

Abstract
The weak line of Hg ii located at 5677.10 Å is used to measure the mercury abundance in the most massive component of the SB2 HgMn star HD 173524 (46 Dra). This line is detected at 3σ above the noise in the adjacent continuum. The synthesis of this line, confirms a large overabundance of mercury, about +5.00 dex, which is less than the excess derived from the 3983.93 Å line in earlier studies. Spectrum synthesis yields estimates of the abundance deficiencies/excesses for sixteen other elements. Helium, carbon, oxygen, magnesium, silicon, chromium and iron are depleted. Phosphorus, titanium, manganese, gallium, strontium, yttrium, zirconium and barium are overabundant. Platinum is very overabundant. For several elements, these new abundances differ from earlier measurements.
Ebi3 Binding to IFN-γ and IL-10 Limits Their Function
Authors: Scott Ellen N , Ye Cheng , Yano Hiroshi , Lipatova Zhanna , Brunazzi Erin , Vignali Kate M , Workman Creg J , Vignali Dario A A 
Published: September 2024

Abstract
EBV-induced gene 3 (Ebi3) is a β subunit within the IL-12 cytokine family that canonically binds to α subunits p19, p28, or p35 to form the heterodimeric cytokines IL-39, IL-27, and IL-35, respectively. In the last decade, the binding partners for Ebi3 have continued to expand to include IL-6 and the other IL-12 family β subunit p40, revealing the possibility that Ebi3 may be able to bind to other cytokines and have distinct functions. We first explored this possibility utilizing an in vivo mouse model of regulatory T cell–restricted deletions of the subunits composing the cytokine IL-35, p35, and Ebi3, and we observed a differential impact on CD8+ T cell inhibitory receptor expression despite comparable reduction in tumor growth. We then screened the ability of Ebi3 to bind to different cytokines with varying structural resemblance to the IL-12 family α subunits. These in vitro screens revealed extracellular binding of Ebi3 to both IFN-γ and IL-10. Ebi3 bound to IFN-γ and IL-10 abrogated signal transduction and downstream functions of both cytokines. Lastly, we validated that extracellular complex formation after mixing native proteins resulted in loss of function. These data suggest that secreted partnerless Ebi3 may bind to cytokines within the extracellular microenvironment and act as a cytokine sink, further expanding the potential immunological impact of Ebi3.
Structure of a Human Monoclonal Antibody in Complex with Outer Surface Protein C of the Lyme Disease Spirochete, Borreliella burgdorferi
Authors: Rudolph Michael J , Chen Yang , Vorauer Clint , Vance David J , Piazza Carol Lyn , Willsey Graham G , McCarthy Kathleen , Muriuki Beatrice , Cavacini Lisa A , Guttman Miklos , Mantis Nicholas J 
Published: September 2024

Abstract
Lyme disease is a tick-borne, multisystem infection caused by the spirochete Borreliella burgdorferi. Although Abs have been implicated in the resolution of Lyme disease, the specific B cell epitopes targeted during human infections remain largely unknown. In this study, we characterized and defined the structural epitope of a patient-derived bactericidal monoclonal IgG (B11) against outer surface protein C (OspC), a homodimeric lipoprotein necessary for B. burgdorferi tick-mediated transmission and early-stage colonization of vertebrate hosts. High-resolution epitope mapping was accomplished through hydrogen deuterium exchange–mass spectrometry and X-ray crystallography. Structural analysis of B11 Fab-OspCA complexes revealed the B11 Fabs associated in a 1:1 stoichiometry with the lateral faces of OspCA homodimers such that the Abs are essentially positioned perpendicular to the spirochete’s outer surface. B11’s primary contacts reside within the membrane-proximal regions of α-helices 1 and 6 and adjacent loops 5 and 6 in one OspCA monomer. In addition, B11 spans the OspCA dimer interface, engaging opposing α-helix 1′, α-helix 2′, and loop 2–3′ in the second OspCA monomer. The B11-OspCA structure is reminiscent of the recently solved mouse transmission blocking monoclonal IgG B5 in complex with OspCA, indicating a mode of engagement with OspC that is conserved across species. In conclusion, we provide a detailed insight into the interaction between a functional human Ab and an immunodominant Lyme disease Ag long considered an important vaccine candidate.
TLR7-Induced Mitochondrial Reactive Oxygen Species Production in Monocyte-derived Dendritic Cells Drives IL-12–Dependent NK Cell Activation and Enhances Antitumor Immunity
Authors: Paladhi Ankush , Daripa Samrat , Nath Arghya , Hira Sumit Kumar 
Published: September 2024

Abstract
Dendritic cell (DC)–based vaccines are promising immunotherapies for cancer. Although DC-based therapies are known to activate tumor-specific T cells, the interplay between DCs and NK cells in this setting is not fully understood. In this study, we demonstrated a novel TLR7/ mitochondrial reactive oxygen species (mROS)/IL-12 axis that drives potent NK cell responses against tumors. We showed that TLR7 activation by imiquimod in peripheral blood monocyte-derived CD11c+ DCs triggered mROS production, leading to enhanced IL-12 secretion and subsequent NK cell activation, as evidenced by increased IFN-γ production and tumor cell cytotoxicity. Notably, mROS neutralization abrogates NK cell–mediated tumor cell lysis, and TLR7-mediated DC activation of NK cells occurs independently of MyD88, suggesting involvement of the noncanonical NF-κB pathway. Our findings provide a rationale for targeting the TLR7/mROS/IL-12 axis to enhance the efficacy of DC-based cancer immunotherapy.
Reconciling the pain with gain of Church leadership conflict in Zimbabwe
Authors: Tagwirei Kimion 
Published: September 2024

Since time immemorial, there has been demonisation of conflict that entangles Church leaders and congregants in Zimbabwe. Innumerable publications have exposed multifarious negative effects of ecclesial conflicts, such as dividing the parties involved, splitting churches and creating space for dubious and cultist opportunists to take up leadership positions at the expense of gullible believers. While this happens, focusing on the negative consequences of conflict prolongs the hurt and pain, while delaying healing, closure, peace and development. Thus, the positive effects of conflict represent a critical gap in need of attention. Employing qualitative literature review, biblical peace and conflict management principles, this submission reconciles the aforementioned downsides with the upsides of Church conflict, such as necessitating introspection and exposing hidden issues that need attention. It finds conflict unavoidable, yet sometimes destructive, constructive and indicative that certain issues must be addressed. All in all, it recommends that Church leaders should not delay, or rub off, but manage conflict and upgrade their conflict management as well as peace building skills in view of biblical precepts to stay progressive in any situation.Contribution: This article reconciles the negative with the positive effects of Church leadership conflicts to achieve ecclesial and community development.
The Efficacy of a 6-month Pain Mentoring Program on Pain Knowledge, Beliefs, and Confidence within the United States Department of Veterans Affairs
Authors: Kurszewski Wesley , Eichelsdoerfer Edward , Eneberg-Boldon Kristin , Gansen Jennifer , Havran Mark , Lahn Molly J. , Mazzone Kerry E. , Schaack Bradley , Schopmeyer Kathryn , Vogsland Rebecca , Keter Damian 
Published: September 2024

Background and Aim:
Pain complaints are one of the primary reasons patients seek musculoskeletal care in the U. S. Veterans Health Administration. While research surrounding the pain experience has rapidly evolved, practicing clinicians often struggle to keep up with evidence-based recommendations and guidelines. The purpose of this project was to explore the efficacy of a 6-month education and mentoring program on physical therapist (PT) and PT assistant pain knowledge and confidence in providing evidence-based pain care.

Materials and Methods:
A 6-month education and mentoring program occurred across three sites with a focus on biopsychosocial pain care principles and treatment based on the mechanism-based classifications of pain. Data collection occurred at baseline, 3 months, and 6 months to include pain knowledge (revised Neurophysiology of Pain Questionnaire [rNPQ]), health-care attitudes and beliefs (Health Care Providers Pain and Impairment Relationship Scale [HC-PAIRS]), self-reported confidence addressing program objectives and self-reported satisfaction in assessing and treating patients with persistent pain.

Results and Conclusion:
Physical therapy professionals (n = 15) demonstrated improved scores on rNPQ and HC-PAIRS with medium effect size at 3 months (d = 0.57; d = 0.73) and 6 months (d = 0.71; d = 0.59). Self-reported confidence addressing program objectives demonstrated improvement across all 13 objectives from baseline to 3 months (0.8 mean diff) and 6 months (1.2 mean diff). The findings from this project support the efficacy of a 6-month education and mentorship program for improving pain knowledge, health-care attitudes and beliefs, and self-rated confidence and satisfaction in treating patients experiencing persistent pain among practicing physical therapy professionals.
Expression of circulating tumour cells in oral squamous cell carcinoma: An ex vivo pilot study
Authors: Sahana N S , Yadava Satish T. , Choudhary Bibha , Ravindran Febina , Khatoon Hajira , Kulkarni Meghashyama 
Published: September 2024

Introduction:
Despite advances in diagnostics and therapeutics, two-thirds of oral cancer patients present with advanced disease, which increases both the morbidity and mortality risk. Circulating tumour cells (CTCs) are released in the circulation by primary tumours and have been demonstrated to have significant correlations between their occurrence and disease progression.

Objectives:
To characterize the circulating tumour cells in subjects with histologically diagnosed oral squamous cell carcinoma (OSCC).

Materials and Methods:
This pilot study was undertaken with ten fresh blood samples (6 ml each). Five samples from apparently healthy individuals and five OSCC samples were cultured and subjected to flow cytometric analysis for CD44 expression. Immunostaining was done using CD44 and EpCAM markers.

Result:
Several cells in OSCC samples showed EpCAM and CD44 positivity following immunostaining. However, flow cytometry performed with CD44 alone was not specific for OSCC samples. Hence, proving that CD44 and EpCAM when used in conjunction can help to characterize CTCs.

Conclusion:
The findings of our study suggest that the demonstration of CTCs is feasible and helps in understanding of disease progression and metastatic risk. Sensitive detection of CTCs from blood samples can serve as an implicit tool in early cancer diagnosis and prognosis through liquid biopsy which in itself is minimally invasive and time-saving.
Relationship between dental fluorosis, dental caries and salivary levels of Streptococcus mutans
Authors: Ravuru Neeharika , Reginald B. Ajay , Reddy Basireddy Siva , Samatha Meda 
Published: September 2024

Background and Objective:
Worldwide, dental caries is an endemic infection and a significant public health problem. Fluoride reduces caries by helping to prevent demineralisation, by remineralising early carious lesions, and by decreasing the activity and growth of Streptococcus mutans (S. mutans). Fluoride intake above the optimal levels leads to a condition known as dental fluorosis, which causes hypo-mineralisation of the tooth. Some studies have revealed that the severity of fluorosis is directly proportional to caries, but some showed opposite results. Hence, with these contradicting results, this study was undertaken to identify the relationship between different grades of dental fluorosis, dental caries and the most common cariogenic bacteria S. mutans in saliva.

Materials and Methods:
A total of 90 subjects within 14- to 16-year age range were selected and categorised depending on the severity of fluorosis into three groups: group I (mild), group II (moderate) and group III (severe) based on modified Dean’s fluorosis index criteria (1942). Unstimulated whole saliva samples were collected using the oral rinse technique and processed for quantification of S. mutans using Mitis Salivarius Bacitracin Agar medium. The number of colony-forming units (CFUs) was determined using a colony counter and expressed as ≥102, ≥103, ≥104 and ≥105 CFUs per ml of the sample, in accordance with the scale recommended by the manufacturer (HiMedia Laboratories). The severity of the caries was measured using decayed, missing or filled teeth (DMFT).

Results:
Observations revealed that the overall DMFT was directly proportional to the level of S. mutans titres. It was observed that 67 to 73% of participants showed fewer colony counts (102 and 103) with minimal DMFT scores and 27 to 33% showed higher counts (104 and 105) with higher DMFT scores in all the groups. 105 CFUs of group III showed the highest mean DMFT scores (2.9) with an increased number of involved subjects than group II (2.3) and group I (1.5). In all, male participants had higher bacterial titres and DMFT scores than females.

Conclusion:
Fluoride in the drinking water served as anticariogenic agent regardless of the severity of fluorosis. Severe fluorosis with a higher incidence of caries and increased CFUs of S. mutans clearly indicates the importance of preventive measures and early treatment to reduce the severity of fluorosis and prevalence of dental caries.
Serum Carboxy-terminal Cross-linking Telopeptide Fragments of Type-2 Collagen Level in the General Population: A Pilot Study
Authors: Khanna Rajat , Jindal Divyam , Singh Sudhir 
Published: September 2024

Abstract

Aim:
The aim of this study was to determine the serum carboxy-terminal cross-linking telopeptide fragments of type-2 collagen (sCTX-II) level in normal healthy young and middle-aged individuals.

Materials and Methods:
Normal healthy adults were included as the participants in this study (175 participants). The age, gender, weight, and height of individuals were recorded. Venous samples (5 ml) were collected for the estimation of the sCTX-II level by ELISA kit.

Results:
The mean sCTX-II level was recorded as 400.42 ± 208.08 pg/ml. The mean sCTX-II level among males was recorded as 406.49 ± 220.79 pg/ml, and in females, it was 382.34 ± 157.88 pg/ml), and the difference is not statistically significant (P = 0.50). The sCTX-II value was recorded as 389.11 ± 199.79 pg/ml in the age group of 19–29 years, 430.45 ± 231.71 pg/ml in the age group of 30–39 years, and 424.12 ± 223.35 pg/ml in the age group of 40–49 years; however, this difference was not statistically significant (P = 0.524). The CTX-II value in the underweight category was 365.55 ± 197.09 pg/ml; in the normal body mass index (BMI) category, it was 404.58 ± 225.29 pg/ml; in the overweight category, the BMI was 392.99 ± 193.79 pg/ml; and in obese BMI category, the value was 417.32 ± 186.79 pg/ml. Our results revealed that the sCTX-II level is independent of age (F = 0.64, P = 0.524), gender (t = 0.66, P = 0.50), and BMI (F = 0.15, P = 0.92).

Conclusion:
We concluded that the sCTX-II level does not get affected by age, gender, and BMI in the general population. The sCTX-II estimation can be used to differentiate the cases of knee osteoarthritis from the normal population as it is independent of age, gender, and BMI. The sCTX-II value in young and middle-aged healthy populations is reported. However, a multicentric study with stringent subject selection criteria is needed to corroborate the results of this pilot study.
Prospective Evaluation of 24 h/2 Days’ Monitoring of Systemic Blood Pressure in Patients with Traumatic Paraplegia – A Single-center Study
Authors: Chaudhary Vandana , Verma N S , Kumar Bhupendra , Rahul Kumar , Kumar Satish 
Published: September 2024

Abstract

Background:
Cardiovascular dysfunction and cognitive decline are more common in traumatic spinal cord injury (SCI). Most of people with a cervical or upper-thoracic SCI usually experience orthostatic hypotension and autonomic dysreflexia, which are described by changes in systemic blood pressure (BP).

Objectives:
The objective of the study is to evaluate baseline heart rate (HR), baseline BP, and ambulatory arterial blood pressure monitoring (ABPM) in traumatic paraplegia patients.

Materials and Methods:
We enrolled 36 patients of traumatic paraplegia patients, paralyzed by Road traffic accident or fall from height within 7 days of trauma. Inclusion criteria were based on Asia Impairment Scale. The BP was monitored for 24 h/2 days and repeated on 12th day of first monitoring. Ambulatory BP was monitored by ABPM machine at ½ hourly from 6 am to 10 pm and 1 hourly from 10 pm to 6 am. The continuous variables were statistically analyzed and compared by t-test.

Results:
The night dipping (Systole) and morning surge (Systole) were significantly lower on 12th day as compared to within 7 days (P = 0.002) whereas the diurnal index (diastole) and diurnal index (mean arterial pressure) were comparable in between within 7 days and 12th day. The mean systolic BP (SBP) maximum and SBP minimum were significantly lower at 12th day as compared to within 7 days, whereas diastolic BP maximum and minimum were comparable in between within 7 days and at 12th day. The changes in minimum, maximum and mean double product (all, active periods, and passive periods) were not found statistically significant in between within 7 days and at 12th day.

Conclusion:
Traumatic paraplegic patients have altered diurnal index, morning surge, night dipping, and SBP on APBM monitoring.
ANALISIS SISTEM KERJA DAN DAMPAK NEGATIF APLIKASI JUDI ONLINE ZEUS DALAM PERSPEKTIF SISTEM INFORMASI
Authors: Julianto Julianto 
Published: September 2024

Gate of Olympus atau yang lebih dikenal di Indonesia Slot Kakek Zeus merupakan platform judi online yang sistem kerjanya akan mengarahkan bola pada gulungan virtual, dimana pemain akan diminta untuk memutar/spin gulungan virtual untuk menciptakan kombinasi simbol yang menghasilkan kelipatan keuntungan dari bola yang tampil atau keuntunggan berupa power boost. Penelitian ini bertujuan untuk mengetahui sistem kerja dan dampak negatif dari permainan judi slot online zeus yang perlu untuk diberantas dan dihindari. Penelitian ini menggunakan pendekatan kualitatif dengan menggunakan metode studi literatur. Adapun hasil dari penelitian ini adalah slot Kakek Zeus menggunakan algoritma Pseudo-Random Number Generators (PRNGs) yang sering dipakai oleh admin atau bandar judi slot online dalam memanipulasi peluang menang dan kalah pemain termasuk mengetahui secara pasti jumlah saldo yang dimiliki pemain, aktivitas permainannya, dan royal atau tidaknya pemain dalam bermain atau membeli scatter dari sistem, sehingga admin atau bandar judi slot dapat mengambil keputusan maupun langkah dalam memancing dan menjebak korbannya untuk dapat terus bermain. Sedangkan dampak negatif yang ditimbulkan oleh platform ini yakni, 1) Judi slot kakek Zeus menimbulkan candu bagi para pemain dikarenakan alunan musik, kerlap kerlip lampu dan tampilan menarik pada permainan memicu pelepasan dopamine seseorang sehingga membuat pemain suka dan selalu ingin memainkannya, yang menyebabkan pemain sulit lepas dari jeratannya. 2) Slot kakek zeus menyebabkan perubahan pada sikap, sifat, dan karakter pemain sehingga menyebabkan pemain lebih emosional saat mengalami kekalahan. 3) Pecandu slot judi menyebabkan pemain sulit untuk mengontrol diri, mengganggu produktivitas kerja, hingga melupakan status dan identitas diri di lingkungan keluarga dan sosial. 4) Kekalahan yang didapat dalam bermain slot judi ini menyebabkan mental dan emosional pemain terganggu, tidak jarang ditemukan beberapa pemain ada yang stress, gila dan bahkan ada yang bunuh diri. 5) Game judi ini memicu pemain untuk berani berhutang dan memicu tindakan kriminal lainnya seperti pencurian dalam pemenuhan hasrat judi slot yang memerlukan deposit dana dalam bermain. 6) Pemain judi slot menjadi sasaran target kiriman SMS dan email spam, WA boom atau blast, dikarenakan saat pendaftaran akun slot judi online ini, pemain diminta untuk memasukan nomor kontak dan email pengguna agar dapat dikirimi berbagai macam tawaran judi slot online lainnya
Telepharmacy module in clinical pharmacy clerkship: Drug-related problem assessment and student reflections
Authors: Karuppannan Mahmathi , Hamdan Nor Elyzatul Akma , Usir Ezlina , Md Saman Kamaliah , Md.Said Siti Norlina , Zulkifly Hanis Hanum , Zaki Izzati Abdul Halim , Noordin Zakiah Mohd 
Published: September 2024

Background: Telepharmacy services received much attention during the COVID-19 pandemic. These services have proven to be a valuable tool in providing remote patient care and ensuring medication safety. However, there is limited research on assessing drug-related problems (DRPs) and the impact of telepharmacy on student learning and reflection.
Objective: To characterise the types of DRPs identified and reflect on students’ telepharmacy modules during final-year clerkship experiences.
Methods: In groups of three to five, final-year students conducted 30-minute telepharmacy sessions, documenting patient details and DRPs on data collection forms under supervision. The data were analysed descriptively, and students' reflections were collected via a Google Form survey.
Results: A total of 187 students completed 48 medication use reviews via telepharmacy. Forty-eight patients were reviewed and 122 DRPs were identified. The average age was 56 years and they were taking an average of five medications. The most common types of DRPs reported during telepharmacy sessions were “non-compliance” (n=42) and “adverse drug reactions (ADR)” (n=30). The main challenges identified by the students throughout the telepharmacy session were unforeseen inquiries and patients with information recall issues.
Conclusion: Under the guidance of their lecturers, pharmacy students identified a significant number of DRPs through telepharmacy medication use review activities.