bluuebunny/crossref_metadata_2025_split huggingface dataset:
Marginal and Internal Fit of CAD/CAM Crowns Fabricated Over Reverse Tapered Preparations
Authors: Carbajal Mejía Jeison B. , Yatani Hirofumi , Wakabayashi Kazumichi , Nakamura Takashi 
Published: December 2017

AbstractPurposeIntraoral digital scanning can accurately record single abutment tooth preparations despite their geometry, and the algorithms of the CAD software can be set to manage different abutment forms. The purpose of this in vitro study was to evaluate the marginal and internal fit of CAD/CAM zirconia crowns fabricated over conventional and reverse‐tapered preparations.Materials and MethodsCrown preparations with known total occlusal convergence (TOC) angles (–8°, –4°, 0°, 8°, 12°, 16°, and 22°) were digitally created from a maxillary left central incisor and printed in acrylic resin. Next, casts were scanned with a TRIOS intraoral scanner, and crowns were designed with KaVo multiCAD software using default parameters (50 μm cement space) on abutments with positive TOC angles, whereas reverse‐tapered abutments (negative TOC angles) were digitally blocked out at 0° and had an extra mesiodistal gap set to 50 μm. Then, zirconia crowns were fabricated, and their marginal and internal discrepancies were recorded with the silicone replica technique. All replicas were examined under a stereomicroscope at 50× magnification. Collected data were analyzed with one‐way ANOVA and post hoc Tukey test for marginal fit. For the axial and incisal fit, measured values did not follow a normal distribution; therefore, the Kruskal‐Wallis and the Dunn/Bonferroni multiple comparison tests were applied (p = 0.05).ResultsThe mean marginal fit of –8° crowns (58.2 ± 6.0 μm) was statistically different (p < 0.0001) from all the remaining crowns (range 42.1‐47.3 μm). Also, the internal fit was statistically significant when comparing crowns fabricated over abutments with positive and negative TOC angles (p < 0.0001). The largest median axial discrepancies were found in the –8° (165.5 μm) and –4° (130.8 μm) groups; however, when evaluating the incisal fit, they showed the smallest discrepancies (67.3 and 81.8 μm, respectively).ConclusionsUnder the conditions of this study, the marginal and internal fit of zirconia crowns fabricated over inverse‐tapered preparations is within clinically accepted values.
Versatile cell surface structures of archaea
Authors: Chaudhury Paushali , Quax Tessa E. F. , Albers Sonja‐Verena 
Published: December 2017

SummaryArchaea are ubiquitously present in nature and colonize environments with broadly varying growth conditions. Several surface appendages support their colonization of new habitats. A hallmark of archaea seems to be the high abundance of type IV pili (T4P). However, some unique non T4 filaments are present in a number of archaeal species. Archaeal surface structures can mediate different processes such as cellular surface adhesion, DNA exchange, motility and biofilm formation and represent an initial attachment site for infecting viruses. In addition to the functionally characterized archaeal T4P, archaeal genomes encode a large number of T4P components that might form yet undiscovered surface structures with novel functions. In this review, we summarize recent advancement in structural and functional characterizations of known archaeal surface structures and highlight the diverse processes in which they play a role.
Prevalence of the AMHR2 mutation in Miniature Schnauzers and genetic investigation of a Belgian Malinois with persistent Müllerian duct syndrome
Authors: Smit MM , Ekenstedt KJ , Minor KM , Lim CK , Leegwater PAJ , Furrow E 
Published: December 2017

ContentsPersistent Müllerian duct syndrome (PMDS) is a sex‐limited disorder in which males develop portions of the female reproductive tract. Important consequences of PMDS are cryptorchidism and its sequelae of infertility and increased risk of testicular cancer. Anti‐Müllerian hormone (AMH) and its receptor (AMHR2) induce the regression of the Müllerian ducts in male embryos. In Miniature Schnauzer dogs, the genetic basis has been identified as an autosomal recessive nonsense mutation in AMHR2, but the allele frequency of the mutation is unknown. Thus, the primary objective of this study was to estimate the prevalence of the AMHR2 mutation in North American Miniature Schnauzers, in order to ascertain the value of genetic testing in this breed. An additional objective was to determine whether mutations in AMH or AMHR2 were responsible for PMDS in a Belgian Malinois; this would aid development of a genetic test for the Belgian Malinois breed. Genomic DNA from 216 Miniature Schnauzers (including one known PMDS case) was genotyped for the AMHR2 mutation, and DNA from a single PMDS‐affected Belgian Malinois was sequenced for all coding exons of AMH and AMHR2. The Miniature Schnauzer cohort had an AMHR2 mutation allele frequency of 0.16 and a carrier genotypic frequency of 0.27. The genetic basis for PMDS in the Belgian Malinois was not determined, as no coding or splicing mutations were identified in either AMH or AMHR2. These findings support a benefit to AMHR2 mutation testing Miniature Schnauzers used for breeding or with cryptorchidism.
Identity construction and language use in a Singaporean Muslim youth camp
Authors: CHEW PHYLLIS GHIM‐LIAN 
Published: December 2017

ABSTRACTThe paper examines how pre‐teen Muslim boys and girls from the same classroom and the same madrasah are faced with differing curricula when the time comes for their annual camp. The choice of activities and corresponding narratives within each camp foregrounds the processual nature of identity construction. This article undertakes a linguistic and semiotic analysis of discoursal narratives in the two camps, which take place a week apart, so as to uncover ideological frameworks in the construction of religious identities. A comparative survey of organizational, curriculum and linguistic practices then focuses on how boys and girls are socialized.
Reviewer Acknowledgements
Authors: Johnson Patricia 
Published: December 2017

Studies in Media and Communication (SMC) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether SMC publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 5, Number 2Anastasia Stamou, University of Western Macedonia, GreeceAndreas Veglis, Aristotle University of Thessaloniki, GreeceAnn Grand, University of the West of England, UKAntonio García Gómez, University of Alcalá de Henares, SpainAslı Sezgin, Osmaniye Korkut Ata University, TurkeyBegoña Montero-Fleta, Universitat Poltècnica de València, SpainBernard Naledzani Rasila, University of Venda, South AfricaCamelia Cmeciu, Danubius University of Galati, RomaniaCarmen Pérez-Sabater, Universitat Politècnica de València, SpainChristian Lamour, The Luxembourg Institute for Socio-Economic Research, LuxembourgDiego Santos Vieira de Jesus, International Relations, BrazilDorina Ticu, Alexandru Ioan Cuza University, RomaniaJenny-Ann Danell, Umea University, SwedenJoerg Tropp, Pforzheim University, GermanyKaterina Diamantaki, Hellenic Open University, GreeceKunle Olufemi Aramide, The Polytechnic Ibadan, NigeriaMaría José Serrano, Universidad de La Laguna, SpainNdoma Brown, Cross River University of Technology, NigeriaNuran E. Isik, Izmir University of Economics, TurkeyRefat Aljumily, Newcastle University, UKSimone Tosoni, Catholic University of Milan, ItalyYi Luo, Montclair State University, USAYoung Joon Lim, University of Texas Rio Grande Valley, USA Patricia JohnsonEditorial AssistantOn behalf of,The Editorial Board of Studies in Media and CommunicationRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://smc.redfame.com
Célula de Büngner
Authors: Nieto Ramírez Luis Eduardo , Tórres Arciniegas Sara Camila , Rios Rosado Elsa Carolina , Torres Jiménez Ivonne Andrea , Camargo Casas Sandy Daniela 
Published: December 2017

Las lesiones del nervio periférico constituyen una condición clínica frecuente; por ello, entender su fisiopatología y los avances en el campo de la regeneración nerviosa es fundamental para brindar el mejor tratamiento a los pacientes. En los últimos años se ha venido dando cada vez mayor importancia a los eventos regenerativos después de la lesión, donde interviene en gran medida una expresión fenotípica única en este proceso, derivada de células ya presentes, fenómeno clave para la recuperación de la función del nervio lesionado. Este artículo revisó la literatura disponible con el objetivo de entender mejor este evento regenerativo y se encontraron procesos celulares y moleculares que suceden en los axones.
Environmental Microbiota Drives Microbial Succession and Metabolic Profiles during Chinese Liquor Fermentation
Authors: Wang Xueshan , Du Hai , Zhang Yan , Xu Yan 
Published: December 2017

ABSTRACTMany microorganisms in the environment participate in the fermentation process of Chinese liquor. However, it is unknown to what extent the environmental microbiota influences fermentation. In this study, high-throughput sequencing combined with multiphasic metabolite target analysis was applied to study the microbial succession and metabolism changes during Chinese liquor fermentation from two environments (old and new workshops). SourceTracker was applied to evaluate the contribution of environmental microbiota to fermentation. Results showed that Daqu contributed 9.10 to 27.39% of bacterial communities and 61.06 to 80.00% of fungal communities to fermentation, whereas environments (outdoor ground, indoor ground, tools, and other unknown environments) contributed 62.61 to 90.90% of bacterial communities and 20.00 to 38.94% of fungal communities to fermentation. In the old workshop, six bacterial genera (Lactobacillus[11.73% average relative abundance],Bacillus[20.78%],Pseudomonas[6.13%],Kroppenstedtia[10.99%],Weissella[16.64%], andPantoea[3.40%]) and five fungal genera (Pichia[55.10%],Candida[1.47%],Aspergillus[10.66%],Saccharomycopsis[22.11%], andWickerhamomyces[3.35%]) were abundant at the beginning of fermentation. However, in the new workshop, the change of environmental microbiota decreased the abundances ofBacillus(5.74%),Weissella(6.64%),Pichia(33.91%),Aspergillus(7.08%), andWickerhamomyces(0.12%), and increased the abundances ofPseudomonas(17.04%),Kroppenstedtia(13.31%),Pantoea(11.41%),Acinetobacter(3.02%),Candida(16.47%), andKazachstania(1.31%). Meanwhile, in the new workshop, the changes of microbial community resulted in the increase of acetic acid, lactic acid, malic acid, and ethyl acetate, and the decrease of ethyl lactate during fermentation. This study showed that the environmental microbiota was an important source of fermentation microbiota and could drive both microbial succession and metabolic profiles during liquor fermentation.IMPORTANCETraditional solid-state fermentation of foods and beverages is mainly carried out by complex microbial communities from raw materials, starters, and the processing environments. However, it is still unclear how the environmental microbiota influences the quality of fermented foods and beverages, especially for Chinese liquors. In this study, we utilized high-throughput sequencing, microbial source tracking, and multiphasic metabolite target analysis to analyze the origins of microbiota and the metabolic profiles during liquor fermentation. This study contributes to a deeper understanding of the role of environmental microbiota during fermentation.
Draft Genome Sequence of Heavy Metal-Resistant Soil Bacterium Serratia marcescens S2I7, Which Has the Ability To Degrade Polyaromatic Hydrocarbons
Authors: Kotoky Rhitu , Singha L. Paikhomba , Pandey Piyush 
Published: December 2017

ABSTRACT

Serratia marcescens
S2I7 is a heavy metal-resistant, polyaromatic hydrocarbon-degrading bacterium isolated from petroleum-contaminated sites. The genome contains one circular chromosome (5,241,555 bp; GC content 60.1%) with 4,533 coding sequences. The draft genome sequence includes specific genetic elements for degradation of hydrocarbons and for heavy metal resistance.
Abstract A21: Inhibition of Sonic Hedgehog Pathway by small molecule inhibitors: Targeting colon cancer stem cells
Authors: Omar Aadilah , Penny Clement , Ruff Paul 
Published: December 2017

Abstract
The existence of Cancer Stem Cells (CSCs) has been proven extensively in recent years. CSCs have been positively identified in, and isolated from colon cancer. They have been found to be resilient to therapy and are highly metastatic and it has been suggested that this could be attributed to the presence of CSCs expressing cell surface CD133. An active Hedgehog-Gli pathway has been detected in human colon carcinoma cells as well as their stem cells. Sonic Hedgehog (Shh) inhibitors such as cyclopamine have been successful in the treatment of other cancer types. CSCs were isolated from HT29 and DLD1 cells using the CD133 surface protein and these cells were further characterized via their expression of key stem cell markers using fluorescence microscopy. These markers included c-Myc, KLF4 and alkaline phosphatase, all of which showed elevated expression levels in the CSC fraction. The growth pattern of CSCs in comparison to non-CSCs was analyzed using cell-impedance (xCELLigence) assays, with clear differences noted in their growth rates. Adhesion assays revealed that although CSCs have a lower proliferation rate, their adhesive potential proved to be greater, possibly due to them being highly metastatic. The effects of the Shh inhibitors cyclopamine and SANT-2 on cellular adhesion of colon CSCs were compared to the Shh pathway control molecule tomatidine, and a marked decrease in adhesion potential was noted. With regard to cell invasion, this was significantly decreased when CSCs were treated with the small molecule inhibitors. In cell migration/scratch assays a similar trend was seen, wherein cell migration was retarded by Hh pathway inhibition. Using confocal microscopy, the cell surface expression of Shh and CD133 was observed following treatment with cyclopamine and while found to significantly reduce Shh expression, CD133 expression remained unaffected. Considering these in vitro results small molecule inhibitors targeting the Shh pathway appear to be promising therapeutic tools for the treatment of metastatic colon CSCs.
Citation Format: Aadilah Omar, Clement Penny, Paul Ruff. Inhibition of Sonic Hedgehog Pathway by small molecule inhibitors: Targeting colon cancer stem cells [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr A21.
Abstract A22: Identifying serum biomarkers for colorectal cancer using immunoproteomics
Authors: Smith Muneerah , Blackburn Jonathan M. 
Published: December 2017

Abstract
The Blackburn lab has developed a highly sensitive and selective native cancer-antigen (CT100+) microarray, which has been used to detect autoantibodies in the blood samples of cancer patients. Although technically advanced, the microarray has biological limitations as we observe a random antibody response in 10-20% of patients with various cancer-types. Our aim is therefore to use immunoproteomics to identify tumor specific antigens (TSAs) which can reproducibly be used for cancer diagnosis and prognosis. We have an archive of 67 cancer and paired normal tissues, with corresponding autologous blood plasma samples, from patients with colorectal cancer (CRC) (Groote Schuur Hospital, South Africa) for identifying novel TSAs. For each patient, we have a set of clinical information which can be used to identify cancer antigens associated with disease pathogenesis. To identify novel cancer antigens, we have developed an immuno-pulldown assay, in which Protein A and Protein G magnetic microbeads are used to selectively capture antibodies from the patient blood plasma. The Ig-bound microbeads were incubated with native cancer and control tissue lysates to capture antigens. After performing an on-bead tryptic digestion, the peptides were eluted and proteins were identified by mass spectrometry, using a Q-Exactive mass analyser. Using the MaxQuant proteomics software, we were able to identify a total of 1276 proteins, of which 360 were unique to the cancer samples. Eight of the 360 cancer-unique proteins were matched to the Tantigen database, a data source and analysis platform for cancer vaccine target discovery. Furthermore, 3 of the 8 proteins identified have been reported to induce antibody responses in cancer patients. In conclusion, we have developed an immuno-pulldown assay that captures and identifies proteins which are unique to cancer tissues, of which several have shown to induce a T- and B-cell response in cancer patients. Although we are able to identify protein candidates of interest, we observe several limitations with the assay, for which we are currently troubleshooting. Once the method is optimized, the newly identified TSAs will be fabricated on to the CT100+ microarray, and validated with patient sera to confirm the presence of cancer-specific antibody response.
Citation Format: Muneerah Smith, Jonathan M. Blackburn. Identifying serum biomarkers for colorectal cancer using immunoproteomics [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr A22.
Abstract B09: KPNB1-mediated nuclear import is required for inflammatory cytokine expression, invasion and survival of cancer cells
Authors: Stelma Tamara , Chi Alicia , Mall Anwar , Govender Dhiren , Leaner Virna D. 
Published: December 2017

Abstract
Karyopherin β1 (KPNB1) is a nuclear transport protein involved in the transport of transcription factors and other proteins containing a nuclear localization sequence (NLS) into the nucleus. Elevated KPNB1 expression has been reported in cancer and transformed cells and is suggested to sustain the increased metabolic and proliferative demands. Transcription factors such as NFκB and AP-1 contain a NLS which is required by KPNB1 for nuclear import. These transcription factors initiate the expression of multiple cytokines and factors associated with inflammation and cancer cell biology. An inflammatory microenvironment, a hallmark of cancer, contributes to factors such as sustained proliferation, invasion and neoangiogenesis. Our study aimed to investigate the effect of inhibiting nuclear import via KPNB1 on cell biology and inflammatory signaling associated with cancer using siRNA and the novel small molecule, Inhibitor of Nuclear Import- 43 (INI-43). Inhibition of KPNB1 lead to reduced migration and invasion of cervical cancer cells while extended inhibition caused decreased proliferation and apoptosis. KPNB1 is essential for the translocation of NFκB into the nucleus as inhibition of nuclear import resulted in its cytoplasmic retention and decreased transcriptional activity of both transcription factors; NFκB and AP-1. DNA-binding studies confirmed a reduced binding-ability or presence of NFκB in the nuclear extract of KPNB1-inhibited cells. Consequently reduced IL-6, IL-1β, TNF-α; and GM-CSF expression, target genes of NFκB and AP-1, was observed. INI-43 significantly inhibited tumor growth in an ectopic xenograft mouse model and histological analysis found the tumor tissue to be less invasive. Immunohistochemical analysis of mice tumors showed reduced overall expression of KPNB1 as well as a shift in cellular localization to be more cytoplasmic following INI-43 treatment. Our study provides evidence that inhibiting KPNB1 has anti-inflammatory and anti-cancer effects and shows promise as a chemotherapeutic target.
Citation Format: Tamara Stelma, Alicia Chi, Anwar Mall, Dhiren Govender, Virna D. Leaner. KPNB1-mediated nuclear import is required for inflammatory cytokine expression, invasion and survival of cancer cells [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr B09.
Abstract B42: An investigation into the synergistic action of chemotherapy and photodynamic therapy (PDT) in resistant human melanoma
Authors: Nsole-Biteghe Fleury Augustin , Ajuwon Olawale , Davids Lester M. 
Published: December 2017

Abstract
Cutaneous melanoma represents the most lethal form of skin cancer and remains refractory to current therapies. Failure of treatment has been attributed to the over-expression of ABC transporters. Alternative combinatorial approaches comprising chemotherapeutics and photodynamic therapy have been proposed to overcome resistance. The aim of this study was therefore to use the chemotherapeutic drug dacarbazine (DTIC), together with PDT on pigmented and unpigmented metastatic melanoma cell lines in vitro to assess efficacy and potentially identify mechanisms of resistance. We show a possible synergism of PDT+DTIC in reducing melanoma cell viability in vitro. At 24h post-treatment, only the unpigmented melanomas were sensitive to DTIC treatment (20-25% death at 1250µM). At 48h, a lethal dose of 50% was reached in these cells in contrast to the pigmented melanoma (20% at 48h). The same trend was observed with the combination therapy (PDT+DTIC) at both timepoints. Furthermore, PDT+DTIC suppressed the self-renewal capacity of both melanoma cell lines. No significant differences in ABCG2 protein expression was found at 24h post-treatment. Overall, these results suggest that ABCG2 transporters may not play a role in melanoma resistance to therapies and thus further work is required to elucidate the clinical efficacy of combination treatments in the fight against skin cancer.
Citation Format: Fleury Augustin Nsole-Biteghe, Olawale Ajuwon, Lester M. Davids. An investigation into the synergistic action of chemotherapy and photodynamic therapy (PDT) in resistant human melanoma [abstract]. In: Proceedings of the AACR International Conference: New Frontiers in Cancer Research; 2017 Jan 18-22; Cape Town, South Africa. Philadelphia (PA): AACR; Cancer Res 2017;77(22 Suppl):Abstract nr B42.