bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Procedural Pain Is Associated with Regionally-Specific Alterations in Thalamic Development in Preterm Neonates
Authors: Duerden Emma G. , Grunau Ruth E. , Guo Ting , Foong Justin , Pearson Alexander , Au-Young Stephanie , Lavoie Raphael , Chakravarty M. Mallar , Chau Vann , Synnes Anne , Miller Steven P. 
Published: December 2017

Very preterm human neonates are exposed to numerous invasive procedures as part of life-saving care. Evidence suggests that repetitive neonatal procedural pain precedes long-term alterations in brain development. However, to date the link between pain and brain development has limited temporal and anatomic specificity. We hypothesized that early exposure to painful stimuli during a period of rapid brain development, before pain modulatory systems reach maturity, will predict pronounced changes in thalamic development, and thereby cognitive and motor function. In a prospective cohort study, 155 very preterm neonates (82 males, 73 females) born 24–32 weeks' gestation underwent two MRIs at median postmenstrual ages 32 and 40 weeks that included structural, metabolic, and diffusion imaging. Detailed day-by-day clinical data were collected. Cognitive and motor abilities were assessed at 3 years, corrected age. The association of early (skin breaks, birth–Scan 1) and late pain (skin breaks, Scans 1–2) with thalamic volumes andN-acetylaspartate (NAA)/choline (Cho), and fractional anisotropy of white-matter pathways was assessed. Early pain was associated with slower thalamic macrostructural growth, most pronounced in extremely premature neonates. Deformation-based morphometry analyses confirmed early pain-related volume losses were localized to somatosensory regions. In extremely preterm neonates early pain was associated with decreased thalamic NAA/Cho and microstructural alterations in thalamocortical pathways. Thalamic growth was in turn related to cognitive and motor outcomes. We observed regionally-specific alterations in the lateral thalamus and thalamocortical pathways in extremely preterm neonates exposed to more procedural pain. Findings suggest a sensitive period leading to lasting alterations in somatosensory-system development.SIGNIFICANCE STATEMENTEarly exposure to repetitive procedural pain in very preterm neonates may disrupt the development of regions involved in somatosensory processing, leading to poor functional outcomes. We demonstrate that early pain is associated with thalamic volume loss in the territory of the somatosensory thalamus and is accompanied by disruptions thalamic metabolic growth and thalamocortical pathway maturation, particularly in extremely preterm neonates. Thalamic growth was associated with cognitive and motor outcome at 3 years corrected age. Findings provide evidence for a developmentally sensitive period whereby subcortical structures in young neonates may be most vulnerable to procedural pain. Furthermore, results suggest that the thalamus may play a key role underlying the association between neonatal pain and poor neurodevelopmental outcomes in these high-risk neonates.
DRG Voltage-Gated Sodium Channel 1.7 Is Upregulated in Paclitaxel-Induced Neuropathy in Rats and in Humans with Neuropathic Pain
Authors: Li Yan , North Robert Y. , Rhines Laurence D. , Tatsui Claudio Esteves , Rao Ganesh , Edwards Denaya D. , Cassidy Ryan M. , Harrison Daniel S. , Johansson Caj A. , Zhang Hongmei , Dougherty Patrick M. 
Published: December 2017

Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse effect experienced by cancer patients receiving treatment with paclitaxel. The voltage-gated sodium channel 1.7 (Nav1.7) plays an important role in multiple preclinical models of neuropathic pain and in inherited human pain phenotypes, and its gene expression is increased in dorsal root ganglia (DRGs) of paclitaxel-treated rats. Hence, the potential of change in the expression and function of Nav1.7 protein in DRGs from male rats with paclitaxel-related CIPN and from male and female humans with cancer-related neuropathic pain was tested here. Double immunofluorescence in CIPN rats showed that Nav1.7 was upregulated in small DRG neuron somata, especially those also expressing calcitonin gene-related peptide (CGRP), and in central processes of these cells in the superficial spinal dorsal horn. Whole-cell patch-clamp recordings in rat DRG neurons revealed that paclitaxel induced an enhancement of ProTx II (a selective Nav1.7 channel blocker)-sensitive sodium currents. Bath-applied ProTx II suppressed spontaneous action potentials in DRG neurons occurring in rats with CIPN, while intrathecal injection of ProTx II significantly attenuated behavioral signs of CIPN. Complementarily, DRG neurons isolated from segments where patients had a history of neuropathic pain also showed electrophysiological and immunofluorescence results indicating an increased expression of Nav1.7 associated with spontaneous activity. Nav1.7 was also colocalized in human cells expressing transient receptor potential vanilloid 1 and CGRP. Furthermore, ProTx II decreased firing frequency in human DRGs with spontaneous action potentials. This study suggests that Nav1.7 may provide a potential new target for the treatment of neuropathic pain, including chemotherapy (paclitaxel)-induced neuropathic pain.SIGNIFICANCE STATEMENTThis work demonstrates that the expression and function of the voltage-gated sodium channel Nav1.7 are increased in a preclinical model of chemotherapy-induced peripheral neuropathy (CIPN), the most common treatment-limiting side effect of all the most common anticancer therapies. This is key as gain-of-function mutations in human Nav1.7 recapitulate both the distribution and pain percept as shown by CIPN patients. This work also shows that Nav1.7 is increased in human DRG neurons only in dermatomes where patients are experiencing acquired neuropathic pain symptoms. This work therefore has major translational impact, indicating an important novel therapeutic avenue for neuropathic pain as a class.
FK506-Binding Protein 12.6/1b, a Negative Regulator of [Ca2+], Rescues Memory and Restores Genomic Regulation in the Hippocampus of Aging Rats
Authors: Gant John C. , Blalock Eric M. , Chen Kuey-Chu , Kadish Inga , Thibault Olivier , Porter Nada M. , Landfield Philip W. 
Published: December 2017

Hippocampal overexpression of FK506-binding protein 12.6/1b (FKBP1b), a negative regulator of ryanodine receptor Ca2+release, reverses aging-induced memory impairment and neuronal Ca2+dysregulation. Here, we tested the hypothesis thatFKBP1balso can protect downstream transcriptional networks from aging-induced dysregulation. We gave hippocampal microinjections ofFKBP1b-expressing viral vector to male rats at either 13 months of age (long-term, LT) or 19 months of age (short-term, ST) and tested memory performance in the Morris water maze at 21 months of age. Aged rats treated ST or LT withFKBP1bsubstantially outperformed age-matched vector controls and performed similarly to each other and young controls (YCs). Transcriptional profiling in the same animals identified 2342 genes with hippocampal expression that was upregulated/downregulated in aged controls (ACs) compared with YCs (the aging effect). Of these aging-dependent genes, 876 (37%) also showed altered expression in agedFKBP1b-treated rats compared with ACs, withFKBP1brestoring expression of essentially all such genes (872/876, 99.5%) in the direction opposite the aging effect and closer to levels in YCs. This inverse relationship between the aging andFKBP1beffects suggests that the aging effects arise fromFKBP1bdeficiency. Functional category analysis revealed that genes downregulated with aging and restored byFKBP1bwere associated predominantly with diverse brain structure categories, including cytoskeleton, membrane channels, and extracellular region. Conversely, genes upregulated with aging but not restored byFKBP1bassociated primarily with glial–neuroinflammatory, ribosomal, and lysosomal categories. Immunohistochemistry confirmed aging-induced rarefaction andFKBP1b-mediated restoration of neuronal microtubular structure. Therefore, a previously unrecognized genomic network modulating diverse brain structural processes is dysregulated by aging and restored byFKBP1boverexpression.SIGNIFICANCE STATEMENTPreviously, we found that hippocampal overexpression of FK506-binding protein 12.6/1b (FKBP1b), a negative regulator of intracellular Ca2+responses, reverses both aging-related Ca2+dysregulation and cognitive impairment. Here, we tested whether hippocampalFKBP1boverexpression also counteracts aging changes in gene transcriptional networks. In addition to reducing memory deficits in aged rats,FKBP1bselectively counteracted aging-induced expression changes in 37% of aging-dependent genes, with cytoskeletal and extracellular structure categories highly associated with theFKBP1b-rescued genes. Our results indicate that, in parallel with cognitive processes, a novel transcriptional network coordinating brain structural organization is dysregulated with aging and restored byFKBP1b.
Attention Is Required for Knowledge-Based Sequential Grouping: Insights from the Integration of Syllables into Words
Authors: Ding Nai , Pan Xunyi , Luo Cheng , Su Naifei , Zhang Wen , Zhang Jianfeng 
Published: December 2017

How the brain groups sequential sensory events into chunks is a fundamental question in cognitive neuroscience. This study investigates whether top–down attention or specific tasks are required for the brain to apply lexical knowledge to group syllables into words. Neural responses tracking the syllabic and word rhythms of a rhythmic speech sequence were concurrently monitored using electroencephalography (EEG). The participants performed different tasks, attending to either the rhythmic speech sequence or a distractor, which was another speech stream or a nonlinguistic auditory/visual stimulus. Attention to speech, but not a lexical-meaning-related task, was required for reliable neural tracking of words, even when the distractor was a nonlinguistic stimulus presented cross-modally. Neural tracking of syllables, however, was reliably observed in all tested conditions. These results strongly suggest that neural encoding of individual auditory events (i.e., syllables) is automatic, while knowledge-based construction of temporal chunks (i.e., words) crucially relies on top–down attention.SIGNIFICANCE STATEMENTWhy we cannot understand speech when not paying attention is an old question in psychology and cognitive neuroscience. Speech processing is a complex process that involves multiple stages, e.g., hearing and analyzing the speech sound, recognizing words, and combining words into phrases and sentences. The current study investigates which speech-processing stage is blocked when we do not listen carefully. We show that the brain can reliably encode syllables, basic units of speech sounds, even when we do not pay attention. Nevertheless, when distracted, the brain cannot group syllables into multisyllabic words, which are basic units for speech meaning. Therefore, the process of converting speech sound into meaning crucially relies on attention.
Cholinergic Overstimulation Attenuates Rule Selectivity in Macaque Prefrontal Cortex
Authors: Major Alex J. , Vijayraghavan Susheel , Everling Stefan 
Published: December 2017

Acetylcholine is released in the prefrontal cortex (PFC) and is a key modulator of cognitive performance in primates. Cholinergic stimulation has been shown to have beneficial effects on performance of cognitive tasks, and cholinergic receptors are being actively explored as promising targets for ameliorating cognitive deficits in Alzheimer's disease. We hypothesized that cholinergic stimulation of PFC during performance of a cognitive task would augment neuronal activity and neuronal coding of task attributes. We iontophoretically applied the general cholinergic receptor agonist carbachol onto neurons in dorsolateral PFC (DLPFC) of male rhesus macaques performing rule-guided prosaccades and antisaccades, a well established oculomotor task for testing cognitive control. Carbachol application had heterogeneous effects on neuronal excitability, with both excitation and suppression observed in significant proportions. Contrary to our prediction, neurons with rule-selective activity exhibited a reduction in selectivity during carbachol application. Cholinergic stimulation disrupted rule selectivity regardless of whether it had suppressive or excitatory effects on these neurons. In addition, cholinergic stimulation excited putative pyramidal neurons, whereas the activity of putative interneurons remained unchanged. Moreover, cholinergic stimulation attenuated saccade direction selectivity in putative pyramidal neurons due to nonspecific increases in activity. Our results suggest excessive cholinergic stimulation has detrimental effects on DLPFC representations of task attributes. These findings delineate the complexity and heterogeneity of neuromodulation of cerebral cortex by cholinergic stimulation, an area of active exploration with respect to the development of cognitive enhancers.SIGNIFICANCE STATEMENTThe neurotransmitter acetylcholine is known to be important for cognitive processes in the prefrontal cortex. Removal of acetylcholine from prefrontal cortex can disrupt short-term memory performance and is reminiscent of Alzheimer's disease, which is characterized by degeneration of acetylcholine-producing neurons. Stimulation of cholinergic receptors is being explored to create cognitive enhancers for the treatment of Alzheimer's disease and other psychiatric diseases. Here, we stimulated cholinergic receptors in prefrontal cortex and examined its effects on neurons that are engaged in cognitive behavior. Surprisingly, cholinergic stimulation decreased neurons' ability to discriminate between rules. This work suggests that overstimulation of acetylcholine receptors could disrupt neuronal processing during cognition and is relevant to the design of cognitive enhancers based on stimulating the cholinergic system.
HUBUNGAN LINGKUNGAN FISIK DENGAN KEBERADAAN JENTIK AEDES PADA AREA BERVEGETASI POHON PISANG
Authors: Susanti Susanti , Suharyo Suharyo 
Published: December 2017

ABSTRAK
Profil Kesehatan Dinas Kota Semarang tahun 2016 menunjukkan bahwa ABJ di wilayah kerja Puskesmas Halmahera 83,00% masih tergolong rendah yaitu masih di bawah target nasional ≥ 95%. Terdapat area dengan vegetasi pohon pisangg yang rapat pada wilayah tersebut. Penelitian ini bertujuan untuk mengetahui hubungan antara tingkat pH, volume air, kelembaban dan jenis pohon pisang dengan keberadaan jentik Aedes aegypti di wilayah kerja puskesmas Halmahera Kota Semarang tahun 2017. Penelitian ini menggunakan metode observasional analitik kuantitatif dengan pendekatan cross sectional, data primer dan data sekunder diolah dan dianalisis menggunakan uji statistik Chi Square. Sampel yang digunakan berjumlah 100 pelepah. Hasil penelitian menunjukkan bahwa terdapat hubungan antara volume air (p=0,023) dan kelembaban (p=0,0001) dengan keberadaan jentik, serta tidak ada hubungan antara tingkat pH (p=0,153) dan jenis pohon pisang (p=0,493) dengan keberadaan jentik.
Kata kunci : keberadaan jentik, tingkat pH, volume air, kelembaban udara, dan jenis perindukan pohon pisang.
ABSTRACT
Health Profile of Semarang City Office in 2016 told that ABJ (larvae presence free number) in work ​​Halmahera Health Center area was 83.00%. It was below the national target of ≥ 95%. This study purposed to know the relationship between pH level, water volume, humidity and banana tree species with the presence of Aedes aegypti larvae in ​​Halmahera Health Center area in 2017. This research used quantitative analytic observational method with cross sectional approach. Data were processed and analyzed using Chi Square test. The samples were 100 midribs. The results showed that there was correlation between water volume (p = 0,023) and humidity (p = 0.0001) with larvae presence, whereas there was no correlation between pH level (p = 0,153) and banana tree species (p = 0,493) larvae presence.
Keywords: presence of larvae, pH level, water volume, humidity, and banana tree species
The effects of nighttime napping on sleep, sleep inertia, and performance during simulated 16 h night work: a pilot study
Authors: Oriyama Sanae , Miyakoshi Yukiko 
Published: December 2017

Abstract

Objectives
This study aimed to elucidate the effects of two naps taken at night on morning waking state and performance.

Methods
The participants were 12 women. The experiment was performed in a laboratory over 2 days (16:00-09:00). In this crossover comparative study, three experimental nap conditions were used (naps from 22:30 to 00:00 and from 02:30 to 03:00 (22:30-NAP), 00:30 to 02:00 and 04:30 to 05:00 (00:30-NAP), and no naps (NO-NAP), respectively). Measurement items were a Visual Analog Scale for sleepiness and fatigue, the Psychomotor Vigilance Test (PVT), and single-digit addition calculations (10 min) every hour for 18 h from 16:00 to 09:00, excluding nap times.

Results
Sleep inertia and sleepiness were noted directly after napping. Less sleepiness and fatigue were noted in the nap groups between 06:00 and 09:00 in the morning than in the NO-NAP condition and PVT response times were faster. Since participants in the nap groups were able to conduct more single-digit addition calculations, the performance of these groups appeared to be superior to that of the NO-NAP condition. Furthermore, the performance of calculations was significantly better in the 00:30-NAP than in the 22:30-NAP.

Conclusions
Taking two naps during a simulated night shift helps improve sleepiness and fatigue and maintain performance. Taking a nap in the early morning appears to be promising for improving the waking state.
A szelektív monoaminoxidáz-B-gátlók helye a Parkinson-kór kezelési stratégiájában a marosvásárhelyi ideggyógyászati klinikák gyakorlatában
Authors: Szász József Attila , Constantin Viorelia , Fazakas Péter Alpár , Blényesi Eszter , Grieb Levente Gábor , Balla Antal , Sárig Mónika , Szegedi Kinga , Bartha Eszter Noémi , Szatmári Szabolcs 
Published: December 2017

Abstract: Introduction: Selective monoamine oxidase B inhibitors have an accurate place in therapeutical strategy of Parkinsons’s disease. In the early stages of the disease, especially in younger patients with milder symptoms, the introduction of levodopa substitution could be efficacious in delaying; in advanced stages they are mainly used to treat motor complications, as an adjunct to levodopa. Aim: The evaluation of therapeutical strategies used in the neurology clinics of Tirgu Mures County Emergency Clinical Hospital in order to define the role of monoamine oxidase B inhibitors. Method: This retrospective study includes all records of patients with Parkinson’s disease hospitalized between 1 January 2003 and 31 December 2016. From the 2194 reports we used data focusing on the therapeutic recommendations. Regarding disease duration, we divided the patients in two groups: less than or equal to 5 years and more than 5 years. Results: From the 1183 patients in first group, 243 received monoamine oxidase inhibitors: 12 as monotherapy, 52 together with dopamine agonists, in 61 cases combined with levodopa. In 118 cases monoamine oxidase inhibitors were combined with levodopa and dopamine agonists. From 582 cases whith Parkinson’s disease for more than 5 years, 195 received monoamine oxidase B inhibitors (selegiline: 10 cases, rasagiline: 185 cases). In 429 cases we did not find accurate data regarding disease duration (selegiline: 5 cases, rasagiline: 93 cases). Conclusion: The use of monoamine oxidase B inhibitors was similar to those found in literature. The treating physicians should utilise more confidently the available therapeutical combinations. Orv Hetil. 2017; 158(51): 2023–2028.
Sprawiedliwość Boga wobec Szatana na podstawie „Jedenastego kazania do ludu” św. Cezarego z Arles
Authors: Pochwat Józef 
Published: December 2017

Cezary z Arles (†543) w jedenastym kazaniu do ludu dokonał precyzyjnego omówienia zagadnienia, dotyczącego sprawiedliwości Boga wobec szatana. W kazaniu tym, dał tak wyczerpujące wyjaśnienie na ten temat, że jest ono swoistego rodzaju traktatem teologicznym. Bóg, jak zauważa, mógł użyć wobec szatana swojej wszechmocy, nie uczynił jednak tego, ponieważ nie chciał naruszyć sprawiedliwości. Bóg jest sprawiedliwy tak wobec szatana, jak i wobec człowieka. Cezary wyjaśnił, że szatan sam siebie zgubił, nikt go do tego nie zmusił, a powodem była zazdrość. Z kolei szatan, jak zauważył Cezary, z zazdrości doradził człowiekowi wybór zła, nie zmusił człowieka do odwrócenia się od Boga, ponieważ człowiek był wolny. Człowiek natomiast, zamiast należeć do Boga, który go stworzył, wybrał propozycję szatana, która sprowadziła na niego śmierć. Cezary pokazał też dwa etapy wyrwania człowieka z niewoli szatana. Pierwszym było Wcielenie, czyli przyjście Chrystusa w ciele podobnym do ciała grzesznego, drugim był krzyż. Sprawiedliwy Chrystus przyjął na siebie niesprawiedliwość i ujawnił zbrodnicze i niesprawiedliwe postępowanie diabła. Szatan w Wielki Piątek, gdy zabił Chrystusa na krzyżu, przekroczył prawo sprawiedliwości. Dopuścił się zbrodni na Jezusie, stał się winny, gdyż skazał na śmierć całkowicie niewinną osobę. Szatan jako winny, musiał przeżyć konfiskatę tego, co zagarnął – człowieka. Chrystus, jak wykazał Cezary, uwolnił rodzaj ludzki spod władzy szatana przez swoją sprawiedliwość. Uczynił to nie jako Bóg, lecz w ludzkim ciele. Sprawiedliwość Chrystusa ujawnia Jego miłosierdzie względem człowieka.
Variación de la masa corporal grasa por antropometría y bioimpedancia en escolares jujeños
Authors: Martinez Jorge Ivan , Dipierri José Edgardo , Bejarano Ignacio , Quispe Yolanda , Alfaro Emma 
Published: December 2017

El objetivo del presente artículo es comparar distintos métodos de cálculo del porcentaje de grasa corporal (%GC) por antropometría y bioimpedancia (BIA) en una población infanto-juvenil jujeña y analizar la variación de la masa grasa (MG), masa magra (MM) y de los índices de masa grasa (IMG) y masa magra (IMM). Se realizó un estudio transversal en 232 escolares de 12 a 17 años de la ciudad de San Salvador de Jujuy. Se relevaron datos de talla, peso y pliegues tricipital y subescapular. Se determinó el Índice de Masa corporal (IMC) y el %GC por BIA (Maltron BF-900) y antropometría (ecuaciones de Deurenberg y Slaugther). Se calculó media y desvío estándar y las diferencias por sexo y edad se establecieron mediante análisis de la varianza. Se una correlación de Pearson entre los distintos métodos utilizados para estimar el %GC y la concordancia analizó con Bland-Altman. Las mujeres presentaron valores superiores de IMC y pliegues bicipital y tricipital y mayores porcentajes de sobrepeso y obesidad. Los %GC más altos se obtuvieron con BIA en mujeres y con Slaugther en varones. Las mujeres registraron valores promedio significativamente más altos del IMG que los varones con los 3 métodos. La correlación más alta del %GC se observó entre BIA y Deurenberg, seguida por Slaugther y Deurenberg, sin embargo no se observó concordancia entre los 3 métodos. Dada la accesibilidad de la estimación del %GC con la ecuación de Deurenberg se propone la aplicación de esta metodología en estudios epidemiológicos de obesidad.