bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflammatory events during food protein‐induced enterocolitis syndrome reactions
Authors: Pecora Valentina , Prencipe Giusi , Valluzzi Rocco , Dahdah Lamia , Insalaco Antonella , Cianferoni Antonella , De Benedetti Fabrizio , Fiocchi Alessandro 
Published: April 2017

AbstractBackgroundAn increasing number of infants are diagnosed with food protein‐induced enterocolitis syndrome (FPIES), a non‐IgE‐mediated food allergy. Until now, T‐cell, food‐specific mechanisms have been hypothesized.MethodsSixteen children (11M, 5F), affected by FPIES from cow's milk, wheat, fruit, rice, and others, experienced 25 acute episodes managed at our emergency department (ED) and eight FPIES reactions during oral food challenges (OFC). We compared the laboratory data in resting conditions, in the absence of infectious diseases, with data collected during the 25 acute ED episodes (blood samples drawn at 2‐12 hours) and the eight positive OFCs (three samples at 2, 6, and 12 hours). The onset of symptoms was used as a reference time point.ResultsIn basal conditions, total IgE, WBC, neutrophil and eosinophil count, CRP, and SGPT were found normal. LDH and SGOT values were high (627.81±97.88 and 45.75±10.26 UI/L, respectively). During ED reactions, LDH and SGOT increased to 794.21±247.28 (P=.028) and 51.08±16.99 UI/L (P=.14) and neutrophils count and CRP to 8.44±3.82×103/μL (P=.0009) and 3.27±5.73 mg/dL (P=.0014), respectively. During positive OFC, LDH and SGOT did not vary significantly; CRP increased from 0.14±0.18 to 2.49±3.65 mg/dL (P=.00189) and neutrophil count from 2.79±1.42 to 7.10±3.98×103/μL (P=.00096).ConclusionsFPIES reactions are characterized by neutrophilia and by a time‐dependent, significant increase in CRP, indicating that inflammatory mechanisms are in place. This suggests new directions for research on FPIES pathogenesis.
Anatomical description of the sciatic nerve block at the subgluteal region in a neonatal cadaver population
Authors: Acar Adrienne A. , Bösenberg Adrian T , van Schoor Albert‐Neels 
Published: April 2017

SummaryIntroductionSciatic nerve blocks provide intraoperative and prolonged postoperative pain management after lower limb surgery (posterior knee, foot, skin graft surgery). Accurate needle placement requires sound anatomical knowledge. Anatomical studies on children are uncommon; most have been performed on adult cadavers. We studied the location of the sciatic nerve at the gluteal level in neonatal cadavers to establish useful anatomical landmarks.MethodsWe identified the sciatic nerve in the gluteal and thigh region of 20 neonatal cadavers. The skin covering the gluteal and thigh region was reflected laterally, and the underlying structures and muscles were identified. We located the sciatic nerve and measured the distance from the nerve to the greater trochanter of the femur and to the tip of the coccyx with a mechanical dial caliper. The total distance between the two landmarks was then recorded.ResultsWe combined measurements from both sides to form a sample size n = 40. The sciatic nerve was 14.9 ± 2.4 mm lateral to the tip of the coccyx. The total distance between the greater trochanter and the tip of the coccyx was 27.3 ± 4.0 mm.ConclusionOur results provide anatomical evidence that the optimal needle insertion point is approximately halfway between the greater trochanter and the tip of the coccyx—a landmark readily palpable in neonates and infants.
UV‐induced somatic mutations elicit a functional T cell response in the YUMMER1.7 mouse melanoma model
Authors: Wang Jake , Perry Curtis J. , Meeth Katrina , Thakral Durga , Damsky William , Micevic Goran , Kaech Susan , Blenman Kim , Bosenberg Marcus 
Published: April 2017

SummaryHuman melanomas exhibit relatively high somatic mutation burden compared to other malignancies. These somatic mutations may produce neoantigens that are recognized by the immune system, leading to an antitumor response. By irradiating a parental mouse melanoma cell line carrying three driver mutations with UVB and expanding a single‐cell clone, we generated a mutagenized model that exhibits high somatic mutation burden. When inoculated at low cell numbers in immunocompetent C57BL/6J mice, YUMMER1.7 (Yale University Mouse Melanoma Exposed to Radiation) regresses after a brief period of growth. This regression phenotype is dependent on T cells as YUMMER1.7 tumors grow significantly faster in immunodeficient Rag1−/− mice and C57BL/6J mice depleted of CD4 and CD8 T cells. Interestingly, regression can be overcome by injecting higher cell numbers of YUMMER1.7, which results in tumors that grow without effective rejection. Mice that have previously rejected YUMMER1.7 tumors develop immunity against higher doses of YUMMER1.7 tumor challenge. In addition, escaping YUMMER1.7 tumors are sensitive to anti‐CTLA‐4 and anti‐PD‐1 therapy, establishing a new model for the evaluation of immune checkpoint inhibition and antitumor immune responses.
Targeting Photochemical Scalpels or Lancets in the Photodynamic Therapy Field—The Photochemist's Role
Authors: Protti Stefano , Albini Angelo , Viswanathan Radhika , Greer Alexander 
Published: April 2017

AbstractThis review covers photochemical approaches aimed at supplementing surgical instruments with handheld photodynamic therapy (PDT) instruments. PDT is not widely used in hospitals, because of the laser equipment and expertise needed, and because insurance policies often do not cover the procedure. Accordingly, this review focuses on advances in photochemistry, photophysics, nanotechnology and miniaturization techniques that may likely inspire the use of handheld instruments in PDT. A takeaway point is that the advent of photochemical scalpels or lancets that deliver reactive oxygen species (ROS) on site may better equip medical practitioners and allow for eradication of tumors or infections in general. Specifically, the review is divided into several sections: sensitizer types, multiphoton and plasmonic topics, sensitizer delivery, light delivery, dosimetry, fiber optics and handheld implements in PDT.
Novel Osmium‐based Coordination Complexes as Photosensitizers for Panchromatic Photodynamic Therapy
Authors: Lazic Savo , Kaspler Pavel , Shi Ge , Monro Susan , Sainuddin Tariq , Forward Sarah , Kasimova Kamola , Hennigar Robie , Mandel Arkady , McFarland Sherri , Lilge Lothar 
Published: April 2017

AbstractCancer remains a major global malaise requiring the advent of new, efficient and low‐cost treatments. Photodynamic therapy, which combines a photosensitizer and photons to produce cytotoxic reactive oxygen species, has been established as an effective cancer treatment but has yet to become mainstream. One of the main limitations has been the paucity of photosensitizers that are effective over a wide range of wavelengths, can exert their cytotoxic effects in hypoxia, are easily synthesized and produce few if any side effects. To address these shortfalls, three new osmium‐based photosensitizers (TLD1822, TLD1824 and TLD1829) were synthesized and their photophysical and photobiological attributes determined. These photosensitizers are panchromatic (i.e. black absorbers), activatable from 200 to 900 nm and have strong resistance to photobleaching. In vitro studies show photodynamic therapy efficacy with both red and near‐infrared light in normoxic and hypoxic conditions, which translated to good in vivo efficacy of TLD1829 in a subcutaneous murine colon cancer model.
Fluorescence Sensor with A New ON1–OFF–ON2 Switching Mechanism Using the Excited State Intermolecular Proton Transfer Reaction of An Anthracene‐diurea Compound
Authors: Matsumoto Hisato , Nishimura Yoshinobu , Arai Tatsuo 
Published: April 2017

AbstractA new photoreaction mechanism of “Three‐state molecular switch” fluorescence sensor based on ON1‐OFF‐ON2 sequence was achieved by anthracene‐diurea compound, which was designed using two phenylurea groups and one anthracene, 9,10BtDSPUA. Photochemical properties of 9,10BtDSPUA and interaction between 9,10BtDSPUA and anion were investigated in detail by absorption, 1H NMR, fluorescence, and fluorescence decay measurements. While the fluorescence of 9,10BtDSPUA in DMSO (ON1) was quenched in the presence of low concentration of acetate anion (OFF), fluorescence enhancement occurred by the addition of high concentration of acetate anion (ON2). This compound forms complex with acetate anion through hydrogen bonding interaction in the ground state resulted in tautomer formation by excited state intermolecular proton transfer (ESIPT) on irradiation. Whereas single coordination of acetate anion to anthracene‐diurea compound may cause fluorescence quenching, full coordination may cause fluorescence enhancement due to suppressing ESIPT. This suppressing ESIPT was occurred by electron‐donating resonance effect between two urea moieties. This study is the first example of ON1‐OFF‐ON2 fluorescence sensor for concentration detection of acetate anion.
Evidence of cross‐stage CD8+ T cell epitopes in malaria pre‐erythrocytic and blood stage infections
Authors: Müller K. , Gibbins M. P. , Matuschewski K. , Hafalla J. C. R. 
Published: April 2017

SummaryMalaria parasites have a complex, multistage life cycle and there is a widely held view that each stage displays a distinct set of antigens presented to the immune system. Yet, molecular analysis of malaria parasites suggests that many putative antigenic targets are shared amongst the different stages. The specificities of these cross‐stage antigens and the functions of the immune responses they elicit are poorly characterized. It is well‐known that CD8+ T cells play opposing immune functions following Plasmodium berghei (Pb) infection of C57BL/6 mice. Whilst these cells play a crucial role in protective immunity against pre‐erythrocytic stages, they are implicated in the development of severe disease during blood stages. Recently, CD8+ T cell epitopes derived from proteins supposedly specific for either pre‐erythrocytic or blood stages have been described. In this brief report, we have compiled and confirmed data that the majority of the mRNAs and/or proteins from which these epitopes are derived display expression across pre‐erythrocytic and blood stages. Importantly, we provide evidence of cross‐stage immune recognition of the majority of these CD8+ T cell epitopes. Hence, our findings provide a resource to further examine the relevance of antigen‐specific cross‐stage responses during malaria infections.
Luteal activity and effect of dietary energy restriction on follicular development in lactating cows
Authors: Comin A , Peric T , Montillo M , Cappa A , Marchi V , Veronesi MC , Prandi A 
Published: April 2017

ContentsThe aim of this research has been to evaluate the presence of anomalies in the ovarian cycle activity during postpartum and to verify whether 72‐hr dietary fasting during the dominance phase, the phase before ovulation, might modify the ovarian follicle population. The presence of anomalies in ovarian cycle activity has been evaluated in 30 Italian Friesian cows starting from 20 days postpartum until 211 days of lactation. Long oestrus and brief dioestrus or scarce luteal activity have been the main anomalies found through measuring progesterone concentrations in the whey. Until 100 days of lactation, the BCS values of the problematic animals have been significantly lower than those in animals with normal ovarian activity. After 100 days of lactation, the ovarian anomalies continued to appear despite the fact that all the animals have reached comparable BCS values. Starting from the results of this trial, the effect of 72‐hr dietary fasting on dominant follicles has been studied in six cows. Ultrasonography revealed that the diameter of the follicles at 71 days postpartum has been significantly lower than at 181 days. A 72‐hr dietary restriction at 101 and 211 days postpartum did not affect the size of the dominant follicle. However, at 101 days postpartum, half of the animals presented follicular cysts. The effect of fasting differed if the animal has been in early postpartum or 211 days of lactation. Further researches are necessary to understand how different metabolic conditions can modify the follicular population but on the other hand the study shows the utility for farmers and field veterinarians of monitoring the resumption of the ovarian cycle postpartum through the whey progesterone concentrations.
Geochemical Behavior of Rare Earth Elements in Weathered Granitic Rocks in Northern Palawan, Philippines
Authors: Padrones Jenielyn T. , Imai Akira , Takahashi Ryohei 
Published: April 2017

AbstractWeathered crust of granitic rocks of two geochemically similar plutons that differ in age of magmatism were investigated in the Philippines. These are the Cretaceous Daroctan Granite and the Middle Miocene Kapoas Granitoid located in Palawan Island, a part of the Palawan Continental Block derived from the continental margin of southeastern Eurasia. Both plutons are composed of biotite granodiorite and biotite granite and are geochemically characterized as ilmenite series, high‐K calc‐alkaline, intermediate I‐ and S‐types, and peraluminous.Light rare earth element (LREE) content of the weathered granitic crusts in the Kapoas Granitoid is higher than that in the Daroctan Granite, and LREE are enriched in the Daroctan Granite compared to the parent granitic rocks. In both plutons, the residual REE‐bearing minerals are mostly monazite. Allanite is only present in the Kapoas Granitoid while the Daroctan Granite contains xenotime. The Kapoas Granitoid pluton shows potential for placer‐type LREE deposit as observed in the elevated LREE content of the stream and beach sediments containing La, Ce, Nd and Sm up to >10,000 ppm. Sequential leaching extraction of REE using eight reagents which selectively dissolved REE with different mode of occurrence (i.e. water soluble, ion‐exchangeable salts, Pb‐displaceable, acid soluble, Mn‐oxide occluded, organically‐bound, amorphous Fe‐oxide occluded, and crystalline Fe‐oxide occluded forms), show that the Daroctan Granite has a potential for ion‐adsorption type REE resource with proportion of extracted REE ranging from 53 to 74 % among ΣREE. Formation of secondary REE‐bearing minerals, such as cerianite formed on the surface of clay minerals, results in enrichment of REE.
Point, Interval and Density Forecasts of Exchange Rates with Time Varying Parameter Models
Authors: Abbate Angela , Marcellino Massimiliano 
Published: April 2017

SummaryWe explore whether modelling parameter time variation improves the point, interval and density forecasts of nine major exchange rates vis-à-vis the US dollar over the period 1976–2015. We find that modelling parameter time variation is needed for an accurate calibration of forecast confidence intervals and is better suited at long horizons and in high volatility periods. The biggest forecast improvements are obtained by modelling time variation in the volatilities of the innovations, rather than in the slope parameters. We do not find evidence that parameter time variation helps to unravel exchange rate predictability by macroeconomic fundamentals. However, an economic evaluation of the various forecast models reveals that controlling for parameter time variation and macroeconomic fundamentals leads to higher portfolios returns, and to higher utility values for investors.
Direct and Indirect Effects of Training Vouchers for the Unemployed
Authors: Huber Martin , Lechner Michael , Strittmatter Anthony 
Published: April 2017

SummaryThe paper evaluates the effects of awarding vouchers for vocational training on the employment outcomes of unemployed voucher recipients in Germany, as well as the potential mechanism through which they operate. This study assesses the direct effects of voucher assignment net of actual redemption, which may be driven by preference shaping and learning about possible human capital investments or simply by the costs of information gathering. Using a formal mediation analysis framework based on sequential conditional independence assumptions and semiparametric matching estimators, our results suggest that the negative short-term and positive long-term employment effects of receiving a voucher are mainly driven by actual training participation. However, the direct effect of just obtaining a voucher is negative over the short run as well. This result points to potential losses in the effectiveness of such training provision systems if individuals decide not to redeem vouchers, as the chances of employment are lower than under non-award over the short run and under redemption over the long run, which makes non-redemption the least attractive option.
Early Holocene transgressive palaeogeography in the Po coastal plain (northern Italy)
Authors: Bruno Luigi , Bohacs Kevin M. , Campo Bruno , Drexler Tina M. , Rossi Veronica , Sammartino Irene , Scarponi Daniele , Hong Wan , Amorosi Alessandro 
Published: April 2017

AbstractTo understand the complex stratigraphic response of a coastal depositional system to rapid eustatic rise and sediment inputs, the evolution of the Adriatic coastline and Po River system, during the post‐glacial (Holocene) transgression, was investigated. The landward migration and evolution of a wave‐dominated estuary was mapped, based on an extensive data set comprising 14 boreholes, 28 core descriptions and 308 piezocone tests, chronologically constrained between 11·5 and 7·0 kyr bp by 137 radiocarbon dates. Palaeogeographic maps reveal temporal differences in retrogradational geometries and mechanisms that likely underpin shoreline retreat. The Po estuary initially developed within a shallowly incised valley and then spread onto the interfluves. Between 11·5 and 9·2 kyr bp the Po fluvial system became avulsive/distributive and wetlands developed in topographically depressed areas. The shoreline retreated at a mean rate of ca 10 m year−1, between 9·2 kyr and 7·7 kyr bp, following a stepped trajectory at the centennial scale. After 7·7 kyr bp, bayhead deltas started to prograde and partially filled the estuary. The overall stratigraphic architecture is interpreted to reflect the sedimentary response of the coastal depositional system to the main pulses of early Holocene eustatic rise. The influence of antecedent topography, partly due to local subsidence, was dominant at the time of initial transgression. Basin morphology influenced sediment dispersal and partitioning. Sediment supplied by the Po River was trapped within the estuary, whereas coastal sand bodies at the estuary mouth were fed by alongshore currents and by reworking of older barriers. High‐resolution age control that ties facies evolution to independently constrained eustasy provides direct data to test models of short‐term coastal retreat under conditions of relative sea‐level rise, and makes this case study a useful analogue for the interpretation of ancient marginal‐marine, retrogradational systems where only stratal geometries are available.
Youth, activism, and social movements
Authors: Earl Jennifer , Maher Thomas V. , Elliott Thomas 
Published: April 2017

AbstractThere has been considerable debate over the extent and role of young people's political participation. Whether considering popular hand‐wringing over concerns about declines in young people's institutional political participation or dismissals of young people's use of online activism, many frame youth engagement through a “youth deficit” model that assumes that adults need to politically socialize young people. However, others argue that young people are politically active and actively involved in their own political socialization, which is evident when examining youth participation in protest, participatory politics, and other forms of noninstitutionalized political participation. Moreover, social movement scholars have long documented the importance of youth to major social movements. In this article, we bring far flung literatures about youth activism together to review work on campus activism; young people's political socialization, their involvement in social movement organizations, their choice of tactics; and the context in which youth activism takes place. This context includes the growth of movement societies, the rise of fan activism, and pervasive Internet use. We argue that social movement scholars have already created important concepts (e.g., biographical availability) and questions (e.g., biographical consequences of activism) from studying young people and urge additional future research.
Rice No Pollen 1 (NP1) is required for anther cuticle formation and pollen exine patterning
Authors: Liu Ze , Lin Sen , Shi Jianxin , Yu Jing , Zhu Lu , Yang Xiujuan , Zhang Dabing , Liang Wanqi 
Published: April 2017

SummaryAngiosperm male reproductive organs (anthers and pollen grains) have complex and interesting morphological features, but mechanisms that underlie their patterning are poorly understood. Here we report the isolation and characterization of a male sterile mutant of No Pollen 1 (NP1) in rice (Oryza sativa). The np1‐4 mutant exhibited smaller anthers with a smooth cuticle surface, abnormal Ubisch bodies, and aborted pollen grains covered with irregular exine. Wild‐type exine has two continuous layers; but np1‐4 exine showed a discontinuous structure with large granules of varying size. Chemical analysis revealed reduction in most of the cutin monomers in np1‐4 anthers, and less cuticular wax. Map‐based cloning suggested that NP1 encodes a putative glucose‐methanol‐choline oxidoreductase; and expression analyses found NP1 preferentially expressed in the tapetal layer from stage 8 to stage 10 of anther development. Additionally, the expression of several genes involved in biosynthesis and in the transport of lipid monomers of sporopollenin and cutin was decreased in np1‐4 mutant anthers. Taken together, these observations suggest that NP1 is required for anther cuticle formation, and for patterning of Ubisch bodies and the exine. We propose that products of NP1 are likely important metabolites in the development of Ubisch bodies and pollen exine, necessary for polymerization, assembly, or both.
Persistent symptomatic parvovirus B19 infection with severe thrombocytopenia transmitted by red blood cell transfusion containing low parvovirus B19 DNA levels
Authors: Nagaharu Keiki , Sugimoto Yuka , Hoshi Yuji , Yamaguchi Takanori , Ito Ryugo , Matsubayashi Keiji , Kawakami Keiki , Ohishi Kohshi 
Published: April 2017

BACKGROUNDTransfusion‐mediated human parvovirus B19 (PVB19) infection is rare but often causes severe hematologic disorders. In Japan, routine blood donor screening for PVB19 antigen (detection sensitivity, 106.4 IU/mL) using a chemiluminescent enzyme immunoassay (CLEIA) was introduced in 2008. However, there is no consensus on the minimal infectious dose of PVB19 permissible for red blood cells (RBCs).CASE REPORTA 64‐year‐old man, who had received hemodialysis for diabetic nephropathy for 5 years, underwent an RBC transfusion for anemia caused by hemorrhagic enterocolitis. He developed persistent high fever and progressive thrombocytopenia. He was diagnosed with PVB19 infection when a marrow examination showed giant erythroblasts, and his serum was positive for PVB19 DNA. His serum was negative for PVB19 immunoglobulin (Ig)M and IgG before transfusion, but positive for both after transfusion. This PVB19 infection was deemed to be transmitted by the RBC transfusion because low levels of PVB19 DNA (1.10 × 104 IU/mL) were detected in one of the blood donors. A DNA homology test of PVB19 showed complete genomic identity between the virus in the donor and our patient.CONCLUSIONWe report a patient who developed persistent PVB19 infection from an RBC transfusion containing low levels of PVB19. This is the second case of transfusion‐mediated PVB19 infection since the introduction of CLEIA in 2008. Transmission may occur in immunocompromised patients lacking PVB19‐neutralizing antibodies. The report of further such cases will allow the establishment of minimal threshold values and more effective screening tests for PBV19 transmission through RBC products.