bluuebunny/crossref_metadata_2025_split huggingface dataset:
Consolidated sustainable organic nanozyme integrated with Point-Of-Use sensing platform for dual agricultural and biological molecule detection
Authors: Lee Dong Hoon , Kamruzzaman Mohammed 
Published: October 2024

AbstractThe newly introduced organic nanozymes offer a promising alternative to inorganic nanozymes by sustainable and cost-effective solutions. Given the potential of nanozyme-based Point-of-Care (PoC) systems for on-demand molecule sensing; these user-friendly Point-of-Use(PoU) platforms can be highly beneficial for real-world agricultural and biological applications. Herein, a novel, fully organic compound/monomer-based, sustainable organic nanozyme is introduced, that exhibits peroxidase-like enzyme-like catalytic activity. Utilizing a unique self-assembled particle fabrication process, homogenous nanozyme are fabricated within a short time (within 50 nm,D90), exhibiting decent kinetic profiles (Km= 0.046 mM, H2O2), and degradability for improved waste management after its use. OM nanozyme-incorporated colorimetric sensing platforms successfully detect popular agricultural toxic biomolecules and biological molecules while achieving decent molecule sensing performance (practical LOD, glyphosate: at least 1pgmL-1, glucose: 3.47 ngmL-1) with high analytical sensitivity and selectivity. Additionally, a smartphone image-based polynomial regression analysis system and paper-microfluidic hardware were developed to facilitate PoU applications in a lab-free environment with satisfactory analytic sensitivity (at least 1.6μgmL-1,for glyphosate, and glucose). This sustainable OM nanozyme, coupled with the PoU platform, is envisioned to have broad applications in agricultural and biological systems.
inPhase — A simple, accurate and fast approach to determine phase diagrams of protein condensates
Authors: Fritsch Anatol W. , Iglesias-Artola Juan M. , Hyman Anthony A. 
Published: October 2024

AbstractProtein phase separation has become a widely studied phenomena in biology with implications in cell metabolism and disease. The study of phase separating proteins often relies on the precise determination of their phase diagrams. These phase diagrams give information on the protein concentration required for condensate formation and the respective concentration inside the condensate at defined external conditions (temperature, salt, pH). However, it has so far often proven difficult to accurately measure phase diagrams. Here, we report a method that is based on mass and volume conservation and defined reaction volumes, which we call inPhase. We can use this method to determine accurate values for both dilute and condensed branch protein concentrations. With this information we can produce accurate phase diagrams. We compare our method to the widely used quantitative fluorescence approach and find that it underestimates the partition factor into condensates at least two-fold for FUS and PGL-3. The accessibility of our method opens the possibility for the thermodynamic assessment of entire protein families, generating sufficient quantitative data for testing theory, and for the screening of drugs in pharmaceutical research.
Motor Imagery Improves Force Control in Older and Young Females
Authors: Calkins Cori A , Kraeutner Sarah N , McNeil Chris J , Jakobi Jennifer M 
Published: October 2024

AbstractMotor imagery training (MIT) is the mental rehearsal of a motor task with no overt movement that enhances physical performance through adaptations in neural excitability. MIT may prime the motor system for physical execution. In older adults, with physical practice, force steadiness (FS) improves and changes are related to improved performance of functional tasks, and associated with adaptations in neural excitability. The purpose of this study was to determine if one session of MIT influences corticospinal excitability and improves FS of isometric elbow flexion contractions in young and older female adults. To test the hypotheses that MIT would increase corticospinal excitability and improve isometric elbow flexion FS to a greater extent in older compared to young females fourteen older (67-89 years old) and twenty-two younger (19-33 years old) participants were randomly assigned to a MIT group or Control group. Participants, in a block design, performed isometric elbow flexion contractions at 10% of maximal force prior to and following MIT (training group) or no training (Control group). Elbow flexion contractions were performed in blocks 1, 3, and 5. MIT or documentary viewing was performed in blocks 2 and 4. Motor evoked potentials (MEPs) elicited by transcranial magnetic stimulation were collected within the last five seconds of each submaximal contraction. The MEPs were reduced in the older MIT group from block 1 to block 5 (p=0.039) but not the young MIT group (p=0.761). Force steadiness in the older (p=0.005) and young (p=0.001) females improved from baseline after 20 minutes of MIT. Older females improved force steadiness relative to the baseline to a greater extent than young females (older 8.44% and young 5.0%), and the improvements were significant in older females in the first 10 minutes. In older females, MIT primes the motor system and improves FS earlier and to a greater extent than in young females.
Serotonin Enhances Neurogenesis Biomarkers, Hippocampal Volumes, and Cognitive Functions in Alzheimer’s Disease
Authors: Azargoonjahromi Ali ,   Alzheimer’s Disease Neuroimaging Initiative
Published: October 2024

AbstractResearch on serotonin reveals a lack of consensus regarding its role in brain volume, especially concerning biomarkers linked to neurogenesis and neuroplasticity, such as ciliary neurotrophic factor (CNTF), fibroblast growth factor 4 (FGF-4), bone morphogenetic protein 6 (BMP-6), and matrix metalloproteinase-1 (MMP-1) in Alzheimer’s disease (AD). This study aimed to investigate the influence of serotonin on brain structure and hippocampal volumes in relation to cognitive functions in AD, as well as its link with biomarkers like CNTF, FGF-4, BMP-6, and MMP-1. Data from the ADNI included 133 AD participants. Cognitive function was assessed using CDR-SB, serotonin levels were measured with the Biocrates AbsoluteIDQ p180 kit and UPLC-MS/MS, and neurotrophic factors and biomarkers were quantified using multiplex targeted proteomics. Voxel-Based Morphometry (VBM) analyzed gray matter volume changes via MRI. Statistical analyses employed Pearson correlation and Bootstrap methods, with p-values < 0.05 or 0.01 considered significant. The analysis revealed a significant positive correlation between serotonin levels and total brain volume (r = 0.179, p = 0.039) and hippocampal volumes (right: r = 0.181, p = 0.037; left: r = 0.217, p = 0.012). Besides, higher serotonin levels were associated with improved cognitive function, evidenced by a negative correlation with CDR-SB scores (r = -0.198, p = 0.023). Furthermore, total brain volume and hippocampal volumes showed significant negative correlations with CDR-SB scores, indicating that greater cognitive impairment was associated with reduced brain volume (total: r = -0.223, p = 0.010; left: r = -0.246, p = 0.004; right: r = -0.308, p < 0.001). Finally, serotonin levels were positively correlated with BMP-6 (r = 0.173, p = 0.047), CNTF (r = 0.216, p = 0.013), FGF-4 (r = 0.176, p = 0.043), and MMP-1 (r = 0.202, p = 0.019), suggesting a link between serotonin and neurogenesis and neuroplasticity. In conclusion, increased serotonin levels are associated with improved cognitive function, increased brain volume, and elevated levels of neurotrophic factors and biomarkers—specifically CNTF, FGF-4, BMP-6, and MMP-1—that are related to neurogenesis and neuroplasticity in AD.Graphical AbstractSerotonin plays a key role in cognitive function, with higher levels linked to increased brain and hippocampal volumes and better cognitive performance, as shown by lower CDR-SB scores (indicating less cognitive impairment). In addition, serotonin is positively associated with neurotrophic factors and biomarkers like ciliary neurotrophic factor (CNTF), fibroblast growth factor 4 (FGF-4), bone morphogenetic protein 6 (BMP-6), and matrix metalloproteinase-1 (MMP-1), which are involved in neurogenesis and neuroplasticity. These findings suggest that elevated serotonin levels contribute to brain health, cognitive function, and the promotion of neural growth and plasticity, particularly in Alzheimer’s disease (AD).
First versatile reverse genetics system for feline coronavirus
Authors: Kida Izumi , Tamura Tomokazu , Kuroda Yudai , Fukuhara Takasuke , Maeda Ken , Matsuno Keita 
Published: October 2024

AbstractFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse genetics system. In the present study, we established a rapid PCR-based system to generate recombinant FCoV using the circular polymerase extension reaction (CPER) method for both serotype 1 and 2 viruses. Recombinant FCoV was successfully rescued at sufficient titers to propagate the progeny viruses with high sequence accuracy. The growth kinetics of recombinant FCoV were comparable to those of the parental viruses. We successfully generated recombinants harboring spike gene from a different FCoV strain or a reporter HiBiT-tag using the CPER method. The chimeric virus demonstrated similar characteristics with the parental virus of S gene. The reporter tag stably expressed after five serial passages in the susceptible cells, and the reporter virus could be applied to evaluate the sensitivity of antiviral inhibitors using the luciferase assay system to detect HiBiT tag. Taken together, our versatile reverse genetics system for FCoV shown herein is a robust tool to characterize viral genes even without virus isolation and to investigate the molecular mechanisms of the proliferation and pathogenicity of FCoV.ImportanceFeline infectious peritonitis is a highly fatal disease in cats caused by feline coronavirus variants that can infect systemically. Because of a lack of versatile toolbox of manipulating the feline coronavirus genome, an efficient method is urgently needed for studying virus proteins responsible for the severe disease. Herein, we established a rapid reverse genetics system for the virus and demonstrated the capability of the recombinant viruses to be introduced desired modifications or reporter genes without any negative impacts on virus characteristics in cell culture. Recombinant viruses are also useful to evaluate antiviral efficacy. Overall, our system can be a promising tool to reveal the molecular mechanisms of viral life cycle of feline coronavirus and disease progression of feline infectious peritonitis.
Immunopeptidomics informs discovery and delivery ofMycobacterium tuberculosisMHC-II antigens for vaccine design
Authors: Leddy Owen , Ogongo Paul , Huffaker Julia , Gan Mingyu , Milligan Ryan , Mahmud Sheikh , Yuki Yuko , Bobosha Kidist , Wassie Liya , Carrington Mary , Liu Qingyun , Ernst Joel D. , White Forest M. , Bryson Bryan D. 
Published: October 2024

AbstractNo currently licensed vaccine reliably prevents pulmonary tuberculosis (TB), a leading cause of infectious disease mortality. Developing effective new vaccines will require identifying which of the roughly 4000 proteins in theMycobacterium tuberculosis(Mtb) proteome are presented on MHC class II (MHC-II) by infected human phagocytes and can be recognized by CD4+ T cells to mediate protective immunity. Vaccines must also elicit T cell responses recognizing the same peptide-MHC complexes presented by infected cells, and successful presentation of target human MHC-II peptides is currently challenging to evaluate and optimize. Here, we define antigenic targets for TB vaccine development by using mass spectrometry (MS) for proteome-wide discovery ofMtbepitopes presented on MHC-II by infected human cells. We next iteratively design and evaluate candidate mRNA vaccine immunogens, revealing design principles that enhance presentation of target MHC-II peptides. Our results will inform the development of new TB vaccine candidates.
The country-of-origin dilemma: domestic bias or need for transparency? Assessing consumer’s value of information for country-of-origin labelling in Italy
Authors: Farina Giuditta , Casati Mirta , Sckokai Paolo 
Published: October 2024

PurposeThe main objective of this study is to analyze consumers’ willingness to pay (WTP) for country-of-origin (COO) labels of two processed food products, disentangling the value of information (VOI) that consumers place on COO information from the value they place on specific countries of origin.Design/methodology/approachA convenience sample of 96 university students completed a face-to-face experiment that included a multiple price list and a discrete choice experiment. Data are analyzed employing interval censored regressions, and random parameter logit models.FindingsOur results indicate that, on average, consumers place a higher value on origin information when a country name is explicitly mentioned. Furthermore, COO information is, on average, more relevant to consumers for products with low involvement than for products with high involvement. Finally, the effect of ethnocentrism is heterogeneous across product categories.Research limitations/implicationsMandatory COO labeling may or may not reinforce domestic bias, depending on the rationale behind consumer support. If consumers are driven by blind ethnocentrism, it may lead to market inefficiencies. However, if they use COO as a cue to align their stated preferences with their choices, it will not. For this alignment to occur, consumers must be fully informed about product attributes, which requires new and smart methods of communicating product attributes.Originality/valueThe present study contributes to the literature on COO food labels, being the first in Italy to disentangle the VOI consumers place on origin information itself from the value they place on specific countries of origin. In addition, it is the first study that applies this methodology across different product categories, each of which has a different level of cultural sensitivity to consumers.
Happy, and they know it? The roles of positive affectivity, intrinsic motivation and network building on LinkedIn on employment predictions
Authors: Harrison Jennifer A. , Halinski Michael , Manroop Laxmikant 
Published: October 2024

PurposeDrawing on trait activation theory, this study examines the influence of positive affectivity on employment predictions (e.g. the probability of obtaining an interview and being hired) via intrinsic motivation and network building on LinkedIn.Design/methodology/approachMultisource field data were collected from student job seekers (n = 179) searching for an internship over two points with a six-month time separation between the first and second data collection.FindingsStructural equation modeling (SEM) analyses revealed marginal support for the mediating roles of intrinsic motivation and network building in positive affectivity’s indirect effect on employment predictions about the probability of obtaining an interview and being hired.Research limitations/implicationsThis study extends research on job search networking/selection by demonstrating the sequential process through which job seekers’ positive affectivity influences employment predictions, emphasizing the intermediary roles of intrinsic motivation and network building on LinkedIn.Practical implicationsJob seekers, recruiters and career counselors should consider network building on LinkedIn as a relevant expression of positive affectivity.Originality/valueWe apply trait activation theory as an overarching framework to examine how an affective between-person difference is expressed via intrinsic motivation and network building and is, at the same time, perceived and valued by employers on LinkedIn.
Enhancing appeal of aggregate payment framing: effects of temporal payment framing and goal proximity on consumer purchase decisions
Authors: Byun Kate Jeonghee 
Published: October 2024

Purpose
The purpose of this paper is to examine how temporal payment framing (i.e. segregate vs aggregate framing) and goal proximity (i.e. proximal vs distal goals) influence consumer purchase decisions.

Design/methodology/approach
Study 1 included four laboratory experiments and examined the moderating role of goal proximity in temporal payment framing. Study 2, using Facebook and a firm’s website for an online field experiment with a data set of 38,731 impressions, extended Study 1, enhancing the generalizability of the findings.

Findings
Consumers exhibited a stronger purchase intention for segregate payment framing. This effect became less pronounced when goal proximity was coupled with temporal payment framing. If products or services emphasized a distal goal, individuals showed greater responses for aggregate payment framing rather than segregate payment framing. The effect was substantial, demonstrating a consistent behavioral pattern in individuals’ browsing choices, including browsing duration and the number of pages viewed.

Research limitations/implications
Future studies ought to investigate if the alignment of goal proximity and temporal payment framing holds consistent across different product types, such as utilitarian versus hedonic products, or search goods versus experience goods.

Practical implications
Marketers can improve the effectiveness and efficiency of their marketing strategies by crafting persuasive messages where the monetary offer is in harmony with the goal proximity.

Originality/value
Temporal payment framing literature has focused on payment framing; however, consumers make their decisions based on a combination of factors rather than solely on payment framing. This study identifies the moderating role of goal proximity.
Mental health at the intersections: understanding South Asian Muslim youth mental health in Peel Region, Toronto, Canada
Authors: Islam Farah , Qasim Kashmala , Qutub Amal , Ali-Mohammed Saamiyah , Abdulwasi Munira , Shakya Yogendra , Hynie Michaela , McKenzie Kwame 
Published: October 2024

Purpose
The purpose of this study was to understand the unique mental health concerns and access barriers experienced by South Asian Muslim youth populations living in the Peel Region of Toronto, Canada.

Design/methodology/approach
For this qualitative exploratory study, interviews (n = 15) were conducted with mental health professionals, educators and spiritual leaders (n = 11) who work with South Asian Muslim youth living in Peel Region, as well as with South Asian Muslim youth themselves (n = 4, aged 20–23). Interview transcripts were analyzed using reflexive thematic analysis.

Findings
Four primary themes emerged from the data: challenges and stressors, barriers, facilitators and hope and recovery. South Asian Muslim youth navigate a number of unique stressors related to the domains of culture, religion and family dynamics, as well as the impact of migration.

Practical implications
The findings stress the necessity of creating culturally safe, multilevel strategies to meet the nuanced challenges and diverse needs of South Asian Muslim youth communities.

Originality/value
This is one of the few papers to the knowledge that addresses the mental health needs and service access barriers of youth populations at the intersections of South Asian diasporic community belonging and Muslim faith in Canada.
Inflation hedging: a comparative wavelet quantile correlation analysis of real estate and alternative assets
Authors: Nasreddine Aya , Essafi Zouari Yasmine 
Published: October 2024

PurposeConducting an analysis spanning from 2000 to 2023, this research evaluates the effectiveness of real estate assets in hedging against global and energy inflation, benchmarked against other compelling investment options such as oil, gold, silver and stocks.Design/methodology/approachThis study employs the wavelet quantile correlation (WQC) methodology. The latter sheds light on dynamic market interactions by scrutinising dependency structures across multiple time scales and also by capturing tail dependence. The adaptability of the wavelet transform, across a spectrum of frequencies, emerges as an indispensable tool for studying time series while also unravelling relationships among variables across diverse quantiles.FindingsThe findings reveal that the response to inflationary pressures is contingent upon the asset class, investment horizon and type of inflation under consideration. While precious metals demonstrate effectiveness over short-term horizons, French-listed real estate exhibits compelling inflation-hedging characteristics as the investment horizon extends. Oil emerges as an unequivocal hedge against both global and energy inflation.Practical implicationsTo counteract the effects of inflation, investors and households may feel compelled to refine their investment strategies, opting to bolster their portfolios with instruments proven to serve as reliable safeguards against inflation, as indicated by this study.Originality/valueIn conjunction with a surge in inflationary pressures, this study delves into the hedging capabilities of assets, exploring their efficacy not only across short- and long-term investment horizons but also within diverse scenarios characterized by fluctuating levels of global and energy-related inflation. To the best of our knowledge, no previous article employed the WQC technique to evaluate the inflation-hedging nexus.
Evolutionary game theoretical approach for reducing carbon emissions in a complex supply chain organization
Authors: Bao Zongke , Wang Chengfang , Innab Nisreen , Mouldi Abir , Ciano Tiziana , Ahmadian Ali 
Published: October 2024

PurposeOur research explores the intricate behavior of low-carbon supply chain organizations in an ever-evolving landscape, emphasizing the profound implications of government-mandated low-carbon policies and the growing low-carbon market. Central to our exploration is applying a combined game theory model, merging Evolutionary Game Theory (EGT) with the Shapley Value Cooperative Game Theory Approach (SVCGTA).Design/methodology/approachWe establish a two-tier supply chain featuring retailers and manufacturers within this novel framework. We leverage an integrated approach, combining strategic Evolutionary Game Theory and Cooperative Game Theory, to conduct an in-depth analysis of four distinct low-carbon strategy combinations for retailers and manufacturers.FindingsThe implications of our findings transcend theoretical boundaries and resonate with a trinity of economic, environmental and societal interests. Our research goes beyond theoretical constructs to consider real-world impacts, including the influence of changes in government low-carbon policies, the dynamics of consumer sensitivities and the strategic calibration of retailer carbon financing incentives and subsidies on the identified ESS. Notably, our work highlights that governments can effectively incentivize organizations to reduce carbon emissions by adopting a more flexible approach, such as regulating carbon prices, rather than imposing rigid carbon caps.Originality/valueOur comprehensive analysis reveals the emergence of an Evolutionary Stability Strategy (ESS) that evolves in sync with the phases of low-carbon technology development. During the initial stages, our research suggests that manufacturers or retailers adopt low-carbon behavior as the optimal approach.