bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2
Authors: Zhang Shihai , Yang Qing , Ren Man , Qiao Shiyan , He Pingli , Li Defa , Zeng Xiangfang 
Published: June 2016

AbstractKnowledge of regulation of glucose transport contributes to our understanding of whole-body glucose homoeostasis and human metabolic diseases. Isoleucine has been reported to participate in regulation of glucose levels in many studies; therefore, this study was designed to examine the effect of isoleucine on intestinal and muscular GLUT expressions. In an animal experiment, muscular GLUT and intestinal GLUT were determined in weaning pigs fed control or isoleucine-supplemented diets. Supplementation of isoleucine in the diet significantly increased piglet average daily gain, enhanced GLUT1 expression in red muscle and GLUT4 expression in red muscle, white muscle and intermediate muscle (P<0·05). In additional, expressions of Na+/glucose co-transporter 1 and GLUT2 were up-regulated in the small intestine when pigs were fed isoleucine-supplemented diets (P<0·05). C2C12 cells were used to examine the expressions of muscular GLUT and glucose uptakein vitro. In C2C12 cells supplemented with isoleucine in the medium, cellular 2-deoxyglucose uptake was increased (P<0·05) through enhancement of the expressions of GLUT4 and GLUT1 (P<0·05). The effect of isoleucine was greater than that of leucine on glucose uptake (P<0·05). Compared with newborn piglets, 35-d-old piglets have comparatively higher GLUT4, GLUT2 and GLUT5 expressions. The results of this study demonstrated that isoleucine supplementation enhanced the intestinal and muscular GLUT expressions, which have important implications that suggest that isoleucine could potentially increase muscle growth and intestinal development by enhancing local glucose uptake in animals and human beings.
Living arrangements and intergenerational monetary transfers of older Chinese
Authors: CHEN TAICHANG , LEESON GEORGE W. , LIU CHANGPING 
Published: June 2016

ABSTRACTPrevious studies show a decline in parent–child co-residence among the elderly. This study examined the effect of living away from adult children on upward intergenerational monetary transfers by analysing a 2006 survey of 19,947 persons aged 60 and above and selected from 20 provinces in China. Results indicate that elderly who were not co-residing but had at least one adult child living in another community/village within the city/county were likely to receive more intergenerational monetary transfers than those who were living with children. Living close to children, rather than co-residing with them, might be the primary living arrangement for older Chinese people in the foreseeable future. The findings have important programme and policy implications for countries such as China, which has the largest elderly population in the world. There is a strong need for the development of specific public care support systems focused on the elderly population, in general, and elderly in rural areas, in particular.
Risk factors associated withCampylobacterdetected by PCR in humans and animals in rural Cambodia
Authors: OSBJER K. , BOQVIST S. , SOKERYA S. , CHHENG K. , SAN S. , DAVUN H. , RAUTELIN H. , MAGNUSSON U. 
Published: June 2016

SUMMARYCampylobacterare worldwide-occurring zoonotic bacteria, with the speciesCampylobacter jejuniandC. colicommonly associated with diarrhoea in children in low-income countries. In this cross-sectional study, the prevalence ofC. jejuniandC. coliin human and livestock faecal samples was detected by PCR and zoonotic risk factors associated with humanCampylobacterpositivity were identified. In total 681 humans and 753 livestock (chickens, ducks, pigs, cattle) from 269 households were sampled. Children aged <16 years were more frequentlyCampylobacterpositive (19%) than adults (8%) and multilevel logistic models revealed that humanC. jejunipositivity was associated with the following household practices: home-slaughtering [odds ratio (OR) 2·4,P= 0·01], allowing animals access to sleeping and food preparation areas (OR 2·8,P= 0·02), and eating undercooked meat (OR 6·6,P= 0·05), while frequent consumption of beef was protective (OR 0·9,P= 0·05). Associations were stronger for home-slaughtering (OR 4·9,P= 0·004) withC. jejuniinfection in children only.Campylobacterwas highly prevalent in pigs (72%) and chickens (56%) and risk factors associated with humanCampylobacterpositivity were identified throughout the meat production chain. The findings underline the importance of studying source attributions throughout the production chain and the need for upgraded understanding ofCampylobacterepidemiology in low-income countries.
Steering Europe: Explaining the Rise of the European Council, 1975–1986
Authors: MOURLON-DRUOL EMMANUEL 
Published: June 2016

AbstractThis article seeks to explain the emergence of the European Council at the heart of Europe's governance between 1975 and 1986. It highlights four factors that quickly made the newly-created institution both indispensable and stable, despite concerns over the excessive reliance on the intergovernmental method in European cooperation processes. These factors were the rise of globalisation in its multi-faceted policy dimensions, a satisfactory new-found institutional balance, the public impact of societal actors’ connections with regular and frequent heads of government meetings and the democratic legitimacy issue in European integration. The article further argues that this period witnessed the de facto emergence of the three-pillar Maastricht structure and shows how the study of the early days of the European Economic Community can shed light on the current development of the European Union and the European Council after the 2009 Lisbon Treaty.
Legume consumption and its association with fasting glucose, insulin resistance and type 2 diabetes in the Indian Migration Study
Authors: Dhillon Preet K , Bowen Liza , Kinra Sanjay , Bharathi Ankalmadugu Venkatsubbareddy , Agrawal Sutapa , Prabhakaran Dorairaj , Reddy Kolli Srinath , Ebrahim Shah ,   for the Indian Migration Study Group
Published: June 2016

AbstractObjectiveLegume consumption is associated with lower fasting glucose (FG) and insulin levels in nutrition trials and lower CVD mortality in large-scale epidemiological studies. In India, legumes are widely consumed in various preparations, yet no epidemiological study has evaluated the association of legumes with FG levels, insulin resistance and diabetes risk. The present study aimed to fill this gap.DesignFasting blood samples, in-person interviews to obtain information on demographic/socio-economic factors, physical activity, alcohol and tobacco use, and anthropometric measurements were collected. Dietary intakes were assessed by an interviewer-administered, validated, semi-quantitative FFQ.SettingLucknow, Nagpur, Hyderabad and Bangalore, India.SubjectsMen and women (n 6367) aged 15–76 years – urban residents, urban migrants and their rural siblings.ResultsIn multivariate random-effects models adjusted for age, BMI, total energy intake, macronutrients, physical activity and rural/migration status, daily legume consumption was not associated with FG (P-for-trend=0·78), insulin resistance (homeostasis model assessment score; P-for-trend=0·73) or the prevalence of type 2 diabetes mellitus (P-for-trend=0·41). Stratified analyses by vegetarian diet and migration status did not change the findings. Inverse associations between legumes and FG emerged for participants with lower BMI and higher carbohydrate, protein, fat and sugar intakes.ConclusionsAlthough legumes are essential in traditional Indian diets, as well as in prudent and Mediterranean diets in the West, we did not find an association between legumes and markers of glycaemic control, insulin resistance or diabetes, except for subgroups based on BMI and macronutrient intake. The ubiquitous presence and complexity of legume preparations in Indian diets may contribute to these findings.
Collagen Fibril Ultrastructure in Mice Lacking Discoidin Domain Receptor 1
Authors: Tonniges Jeffrey R. , Albert Benjamin , Calomeni Edward P. , Roy Shuvro , Lee Joan , Mo Xiaokui , Cole Susan E. , Agarwal Gunjan 
Published: June 2016

AbstractThe quantity and quality of collagen fibrils in the extracellular matrix (ECM) have a pivotal role in dictating biological processes. Several collagen-binding proteins (CBPs) are known to modulate collagen deposition and fibril diameter. However, limited studies exist on alterations in the fibril ultrastructure by CBPs. In this study, we elucidate how the collagen receptor, discoidin domain receptor 1 (DDR1) regulates the collagen content and ultrastructure in the adventitia of DDR1 knock-out (KO) mice. DDR1 KO mice exhibit increased collagen deposition as observed using Masson’s trichrome. Collagen ultrastructure was evaluated in situ using transmission electron microscopy, scanning electron microscopy, and atomic force microscopy. Although the mean fibril diameter was not significantly different, DDR1 KO mice had a higher percentage of fibrils with larger diameter compared with their wild-type littermates. No significant differences were observed in the length of D-periods. In addition, collagen fibrils from DDR1 KO mice exhibited a small, but statistically significant, increase in the depth of the fibril D-periods. Consistent with these observations, a reduction in the depth of D-periods was observed in collagen fibrils reconstituted with recombinant DDR1-Fc. Our results elucidate how DDR1 modulates collagen fibril ultrastructure in vivo, which may have important consequences in the functional role(s) of the underlying ECM.
Taken lives matter: open justice and recognition in inquests into deaths at the hands of the state
Authors: McIntosh Sam 
Published: June 2016

AbstractLord Neuberger describes open justice as a procedural principle requiring that ‘what goes on in court and what a court decides is open to scrutiny’ (Neuberger, 2011). The prime rationale given for this principle is that it is a safety check on procedural fairness. Such a conception of open justice applies on only a superficial level in inquests into use-of-force deaths at the hands of the state. This paper examines the practice of, and rationales behind, opening up use-of-force deaths at the hands of the state to scrutiny through inquests. They suggest a primarily intrinsic rather than instrumental link between openness and inquests’ purposes, which requires a reframing of traditional conceptions of open justice in this context. It is further argued that recognition theory can provide the normative link between openness and justice in these circumstances – a link that is implicit in the term ‘open justice’ but rarely explored in inquests.
Exploring fatal facts: current issues in coronial law, policy and practice
Authors: Scott Bray Rebecca , Martin Greg 
Published: June 2016

AbstractDeath investigation and coronial practices have undergone significant social, political and legal scrutiny in recent years. A wave of coronial reform has occurred across jurisdictions, including in the United Kingdom (UK), Australia, Canada and New Zealand, with a concomitant focus on the adequacy of death investigation law and policy. Taking key coronial developments in the UK and Australia as its starting point, this paper explores a legal jurisdiction undergoing immense legal and policy reform to illustrate why coronial law and practice is of increasing scholarly interest. It begins by tracing the contentious landscape of UK coronial law reform, which has also resonated internationally, thereafter examining key controversies that refocused attention on the value of the jurisdiction, before discussing contemporary coronial issues including, publicity, human rights and death prevention.
Rare disruptive mutations and their contribution to the heritable risk of colorectal cancer
Authors: Chubb Daniel , Broderick Peter , Dobbins Sara E. , Frampton Matthew , Kinnersley Ben , Penegar Steven , Price Amy , Ma Yussanne P. , Sherborne Amy L. , Palles Claire , Timofeeva Maria N. , Bishop D. Timothy , Dunlop Malcolm G. , Tomlinson Ian , Houlston Richard S. 
Published: June 2016

AbstractColorectal cancer (CRC) displays a complex pattern of inheritance. It is postulated that much of the missing heritability of CRC is enshrined in high-impact rare alleles, which are mechanistically and clinically important. In this study, we assay the impact of rare germline mutations on CRC, analysing high-coverage exome sequencing data on 1,006 early-onset familial CRC cases and 1,609 healthy controls, with additional sequencing and array data on up to 5,552 cases and 6,792 controls. We identify highly penetrant rare mutations in 16% of familial CRC. Although the majority of these reside in known genes, we identify POT1, POLE2 and MRE11 as candidate CRC genes. We did not identify any coding low-frequency alleles (1–5%) with moderate effect. Our study clarifies the genetic architecture of CRC and probably discounts the existence of further major high-penetrance susceptibility genes, which individually account for >1% of the familial risk. Our results inform future study design and provide a resource for contextualizing the impact of new CRC genes.
ASC filament formation serves as a signal amplification mechanism for inflammasomes
Authors: Dick Mathias S. , Sborgi Lorenzo , Rühl Sebastian , Hiller Sebastian , Broz Petr 
Published: June 2016

AbstractA hallmark of inflammasome activation is the ASC speck, a micrometre-sized structure formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which consists of a pyrin domain (PYD) and a caspase recruitment domain (CARD). Here we show that assembly of the ASC speck involves oligomerization of ASCPYDinto filaments and cross-linking of these filaments by ASCCARD. ASC mutants with a non-functional CARD only assemble filaments but not specks, and moreover disrupt endogenous specks in primary macrophages. Systematic site-directed mutagenesis of ASCPYDis used to identify oligomerization-deficient ASC mutants and demonstrate that ASC speck formation is required for efficient processing of IL-1β, but dispensable for gasdermin-D cleavage and pyroptosis induction. Our results suggest that the oligomerization of ASC creates a multitude of potential caspase-1 activation sites, thus serving as a signal amplification mechanism for inflammasome-mediated cytokine production.
The biogeography of red snow microbiomes and their role in melting arctic glaciers
Authors: Lutz Stefanie , Anesio Alexandre M. , Raiswell Rob , Edwards Arwyn , Newton Rob J. , Gill Fiona , Benning Liane G. 
Published: June 2016

AbstractThe Arctic is melting at an unprecedented rate and key drivers are changes in snow and ice albedo. Here we show that red snow, a common algal habitat blooming after the onset of melting, plays a crucial role in decreasing albedo. Our data reveal that red pigmented snow algae are cosmopolitan as well as independent of location-specific geochemical and mineralogical factors. The patterns for snow algal diversity, pigmentation and, consequently albedo, are ubiquitous across the Arctic and the reduction in albedo accelerates snow melt and increases the time and area of exposed bare ice. We estimated that the overall decrease in snow albedo by red pigmented snow algal blooms over the course of one melt season can be 13%. This will invariably result in higher melt rates. We argue that such a ‘bio-albedo’ effect has to be considered in climate models.
The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels
Authors: Bavi Navid , Cortes D. Marien , Cox Charles D. , Rohde Paul R. , Liu Weihong , Deitmer Joachim W. , Bavi Omid , Strop Pavel , Hill Adam P. , Rees Douglas , Corry Ben , Perozo Eduardo , Martinac Boris 
Published: June 2016

AbstractThe bacterial mechanosensitive channel MscL gates in response to membrane tension as a result of mechanical force transmitted directly to the channel from the lipid bilayer. MscL represents an excellent model system to study the basic biophysical principles of mechanosensory transduction. However, understanding of the essential structural components that transduce bilayer tension into channel gating remains incomplete. Here using multiple experimental and computational approaches, we demonstrate that the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane. We propose that this may also represent a common principle in the gating cycle of unrelated mechanosensitive ion channels, allowing the coupling of channel conformation to membrane dynamics.
Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-β signalling
Authors: Mirzamohammadi Fatemeh , Papaioannou Garyfallia , Inloes Jennifer B. , Rankin Erinn B. , Xie Huafeng , Schipani Ernestina , Orkin Stuart H. , Kobayashi Tatsuya 
Published: June 2016

AbstractPolycomb repressive complex 2 (PRC2) controls maintenance and lineage determination of stem cells by suppressing genes that regulate cellular differentiation and tissue development. However, the role of PRC2 in lineage-committed somatic cells is mostly unknown. Here we show that Eed deficiency in chondrocytes causes severe kyphosis and a growth defect with decreased chondrocyte proliferation, accelerated hypertrophic differentiation and cell death with reduced Hif1a expression. Eed deficiency also causes induction of multiple signalling pathways in chondrocytes. Wnt signalling overactivation is responsible for the accelerated hypertrophic differentiation and kyphosis, whereas the overactivation of TGF-β signalling is responsible for the reduced proliferation and growth defect. Thus, our study demonstrates that PRC2 has an important regulatory role in lineage-committed tissue cells by suppressing overactivation of multiple signalling pathways.
Structural basis of suppression of host translation termination by Moloney Murine Leukemia Virus
Authors: Tang Xuhua , Zhu Yiping , Baker Stacey L. , Bowler Matthew W. , Chen Benjamin Jieming , Chen Chen , Hogg J. Robert , Goff Stephen P. , Song Haiwei 
Published: June 2016

AbstractRetroviral reverse transcriptase (RT) of Moloney murine leukemia virus (MoMLV) is expressed in the form of a large Gag–Pol precursor protein by suppression of translational termination in which the maximal efficiency of stop codon read-through depends on the interaction between MoMLV RT and peptidyl release factor 1 (eRF1). Here, we report the crystal structure of MoMLV RT in complex with eRF1. The MoMLV RT interacts with the C-terminal domain of eRF1 via its RNase H domain to sterically occlude the binding of peptidyl release factor 3 (eRF3) to eRF1. Promotion of read-through by MoMLV RNase H prevents nonsense-mediated mRNA decay (NMD) of mRNAs. Comparison of our structure with that of HIV RT explains why HIV RT cannot interact with eRF1. Our results provide a mechanistic view of how MoMLV manipulates the host translation termination machinery for the synthesis of its own proteins.
The effect of umeclidinium added to inhaled corticosteroid/long-acting β2-agonist in patients with symptomatic COPD: a randomised, double-blind, parallel-group study
Authors: Sousa Ana R , Riley John H , Church Alison , Zhu Chang-Qing , Punekar Yogesh S , Fahy William A 
Published: June 2016

AbstractBenefits of triple therapy with a long-acting muscarinic antagonist (LAMA), added to inhaled corticosteroid (ICS)/long-acting β2-agonist (LABA), have been demonstrated. Limited data assessing the efficacy of the LAMA umeclidinium (UMEC) added to ICS/LABA are available. The aim of this study is to evaluate the efficacy and safety of UMEC added to ICS/LABAs in patients with moderate-to-very-severe COPD. This is a multicentre, randomised, double-blind, parallel-group study. Patients were symptomatic (modified Medical Research Council Dyspnoea Scale score ⩾2), despite receiving ICS/LABA (fluticasone propionate/salmeterol (FP/SAL, branded) 500/50 mcg, budesonide/formoterol (BD/FOR, branded) 200/6 mcg or 400/12 mcg, or other ICS/LABAs) ⩾30 days before the run-in (7±2 days). Patients were randomised 1:1 to once-daily UMEC 62.5 mcg or placebo (PBO), added to twice-daily open-label ICS/LABA for 12 weeks. Primary end point was trough forced expiratory volume in 1 s (FEV1) at Day 85; secondary end point was weighted mean (WM) 0–6 h FEV1 at Day 84; other end points included COPD Assessment Test (CAT) score and Transition Dyspnoea Index (TDI) score. Adverse events (AEs) were investigated. In the UMEC+ICS/LABA and PBO+ICS/LABA groups, 119 and 117 patients were randomised, respectively. Patients received FP/SAL (40%), BD/FOR (43%) and other ICS/LABAs (17%). UMEC+ICS/LABA resulted in significant improvements in trough FEV1 (Day 85) and in WM 0–6 h FEV1 (Day 84) versus PBO+ICS/LABA (difference: 123 and 148 ml, respectively, both P<0.001). Change from baseline for UMEC+ICS/LABA versus PBO+ICS/LABA was significantly different for CAT score at Day 84 (−1.31, P<0.05), but not for TDI score (0.40, P=0.152). AE incidence was similar with UMEC+ICS/LABA (38%) and PBO+ICS/LABA (42%). UMEC+ICS/LABA improved lung function and CAT score in patients with symptomatic COPD versus PBO+ICS/LABA (ClinicalTrials.gov NCT02257372).
Ketoacidosis at first presentation of type 1 diabetes mellitus among children: a study from Kuwait
Authors: Shaltout Azza Aly , Channanath Arshad Mohamed , Thanaraj Thangavel Alphonse , Omar Dina , Abdulrasoul Majedah , Zanaty Nabila , Almahdi Maria , Alkandari Hessa , AlAbdulrazzaq Dalia , d’Mello Linda , Mandani Fawziya , Alanezi Ayed , AlBasiry Eman , Alkhawari M. 
Published: June 2016

AbstractWe examined the frequency and severity of diabetic ketoacidosis (DKA) in 679 children and adolescents (0–14 years) at diagnosis of Type 1 Diabetes Mellitus (T1DM) in Kuwait. Between 1st January 2011 and 31st December 2013, all newly diagnosed children with diabetes were registered prospectively in a population-based electronic register. DKA was diagnosed using standard criteria based on the levels of venous pH and serum bicarbonate. At the time of diagnosis, mild/moderate DKA was present in 24.8% of the children, while severe DKA was present in 8.8%. Incidence of ketoacidosis was significantly higher in young children less than 2 (60.7% vs 32.4% p = <0.005) compared to children 2–14 years old and a higher proportion presented with severe DKA (21.4% vs 8.3% p = <0.05). No association was seen with gender. Significant differences were found in the incidence of DKA between Kuwaiti and non-Kuwaiti children (31.1% vs 39.8%; p < 0.05). Family history of diabetes had a protective effect on the occurrence of DKA (OR = 0.44; 95% CI = 0.27–0.71). Incidence of DKA in children at presentation of T1DM remains high at 33.6%. Prevention campaigns are needed to increase public awareness among health care providers, parents and school teachers in Kuwait.