bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening and Functional Evaluation of Four Larix kaempferi Promoters
Authors: Zhang Chen-Yi , Ye Zha-Long , Qi Li-Wang , Yang Ling , Li Wan-Feng 
Published: October 2024

Promoters are powerful tools for breeding new varieties using transgenic technology. However, the low and unstable expression of target genes is still a limiting factor in Larix kaempferi (Lamb.) Carr (Japanese larch) genetic transformation. In this study, we analyzed L. kaempferi transcriptome data, screened out highly expressed genes, cloned their promoters, and constructed plant expression vectors containing the β-glucuronidase (GUS) reporter gene driven by these promoters. Recombinant vectors were introduced into the L. kaempferi embryogenic callus by means of the Agrobacterium-mediated transient or stable genetic transformation method, and the promoter activity was then determined by measuring GUS expression and its enzyme activity in the transformed materials. Four highly expressed genes were identified: L. kaempferi Zhang Chen Yi-1 (LaZCY-1), Zhang Chen Yi-2 (LaZCY-2), Translationally Controlled Tumor Protein (LaTCTP), and ubiquitin (LaUBQ). The 2000 bp fragments upstream of ATG in these sequences were cloned as promoters and named pLaZCY-1, pLaZCY-2, pLaTCTP, and pLaUBQ. Semi-quantitative and quantitative RT-PCR analyses of transient genetic transformation materials showed that all four promoters could drive GUS expression, indicating that they have promoter activities. Semi-quantitative and quantitative RT-PCR analyses and the histochemical staining of stable genetic transformation materials showed that the pLaUBQ promoter had higher activity than the other three L. kaempferi promoters and the CaMV35S promoter. Thus, the pLaUBQ promoter was suggested to be used in larch genetic transformation.
Language of primary medical qualification and differential MRCGP exam attainment: an observational study
Authors: Brown Victoria Tzortziou , Haviland Joanne , Priyadarshini Garima , Turner Melody , George Riya Elizabeth , Siriwardena Aloysius Niroshan , Gregory Simon 
Published: October 2024

BackgroundInternational medical graduates (IMGs) comprise more than half of GP registrars but are more likely to fail postgraduate assessments than UK graduates.AimTo assess whether there is an association between the language of the primary medical qualification (PMQ) and Membership of the Royal College of General Practitioners (MRCGP) results, and whether performance in previous prequalification assessments is correlated.Design and settingRetrospective observational study in the UK.MethodThe World Directory of Medical Schools and the UK Medical Education databases were used to obtain data for all candidates who sat the MRCGP exams between October 2013 and July 2021 (n= 28 020). Candidates were split into three cohorts: cohort 1 comprised UK graduates; cohort 2 comprised IMGs with English as the language of the PMQ, who trained in countries with English (cohort 2a) or non-English (cohort 2b) as a first language; and cohort 3 included IMGs with non-English as the language of the PMQ. Logistic and linear regression analyses were used to compare the odds of exam passing and the scores relative to pass. Associations with past Multi-Specialty Recruitment Assessment (MSRA) scores, International English Language Testing System (IELTS) scores, and Professional and Linguistic Assessments Board (PLAB) scores were examined.ResultsIMGs who trained in countries with non-English as a first language had statistically significantly lower odds of passing the exams and lower exam scores across all exam components. There were statistically significant positive correlations between MRCGP exam scores and MSRA, IELTS, and PLAB scores.ConclusionEnglish being the language of the PMQ and undertaking medical training in a country with English as the native language seemed to result in statistically significantly better chances of passing the exams and better exam scores. Performance in prequalification assessments can help to identify those IMG registrars who may benefit from tailored support.
Controle de qualidade microbiológico de água em uma clínica escola de odontologia do sul do Brasil
Authors: Schmitt Ana Paula Iatchac , Silva Michelle dos Santos da , Voltolini Adrielli Tenfen 
Published: October 2024

A transmissão de microrganismos pode ser viabilizada no manejo odontológico, através do uso da água contaminada. Esta contaminação pode se dar através dos equipamentos utilizados, que são irrigados por esta água. O objetivo deste estudo foi realizar o controle de qualidade microbiológico desta água, analisando a incidência dos agentes patogênicos, formadores de biofilme. Foram coletadas amostras dos reservatórios de água individual de vinte e cinco cadeiras de odontologia de uma clínica escola de Odontologia. As amostras foram coletadas em triplicata, em dias alternados e posteriormente foram avaliadas separadamente. Foi avaliado a presença de coliformes fecais e de bactérias patogênicas em todas as amostras. Os resultados expressam uma média dos valores obtidos para a triplicata de cada amostra. Dentre as 25 cadeiras, nove apresentaram resultado positivo para presença de microrganismos, e em nenhuma ultrapassou o limite de coliformes fecais. Os microrganismos identificados foram Staphilococus aureus, pseudomonas aeruginosa e Escherichia coli.
Aplicação da inteligência artificial no diagnóstico e monitoramento do Câncer de Mama: uma revisão sistemática
Authors: Pellenz Alexandro Emédio , Barbosa Júllia Santana da Silva , Geraldes Jordi Guimarães dos Santos , Alves Pietro Matheus Bompet Fontoura , Vasconcelos Juliana 
Published: October 2024

Introdução: O câncer de mama é uma das neoplasias mais prevalentes e a segunda principal causa de morte por câncer entre mulheres em todo o mundo. Devido à sua heterogeneidade genética e molecular, o diagnóstico e o tratamento eficazes representam desafios significativos. A inteligência artificial (IA) tem emergido como uma ferramenta promissora para melhorar a precisão diagnóstica e o monitoramento do câncer de mama, oferecendo potencial para análises mais profundas e personalizadas. Objetivo: Revisar a literatura sobra a eficácia da Inteligência Artificial no diagnóstico e monitoramento do câncer de mama. Métodos: Este trabalho é uma revisão sistemática, seguindo fielmente os critérios estabelecidos pela checklist PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). A pesquisa ocorreu por meio da consulta de artigos científicos publicados nas seguintes bases de dados médicas e científicas: PubMed, SciELO (Scientific Electronic Library Online) e a Biblioteca Virtual em Saúde (BVS). A estratégia de busca adotada empregou palavras-chave previamente definidas, quais sejam: “inteligência artificial”, “aprendizado de máquina”, “câncer de mama”, “diagnóstico e monitoramento”. Resultados: Foram identificados na busca inicial nas bases de dados citadas 47 artigos, com leitura dos títulos e resumos por três avaliadores. A partir da análise de elegibilidade, o avaliador 1 decidiu pela inclusão de 17 artigos (36,17%), a avaliadora 2 de 17 artigos (36,17%) e o avaliador 3 de 19 artigos (40,43%). Discussão: A utilização da inteligência artificial tem revolucionado diagnóstico, o tratamento e o prognóstico de pacientes com câncer de mama. Esse recurso desempenha um papel crucial como uma ferramenta de suporte à decisão e, portanto, auxiliando na prevenção de descuidos por especialistas médicos. Conclusão: Os resultados indicam que a inteligência artificial tem um papel significativo na melhoria da precisão e eficácia no diagnóstico e monitoramento do câncer de mama. Algoritmos de aprendizado de máquina, especialmente RNCs, MVSs, radiômica e termômica, demonstram alta acurácia e sensibilidade quando comparados aos métodos tradicionais de imagem. A adoção dessas tecnologias pode levar a diagnósticos mais precoces e tratamentos mais eficazes, potencialmente reduzindo a mortalidade associada ao câncer de mama.
Produção de composto orgânico para fertilização de horta comunitária
Authors: Zanotto Gabriel , Mann Mariele da Silva Mazuim , Faccin Christiane , Silva Rodrigo Sanchotene 
Published: October 2024

O crescimento populacional e os meios de produção, ao longo dos anos, têm pressionado a humanidade a buscar soluções para excesso de geração de resíduos sólidos orgânicos e o aumento na produção de alimentos, devido as estas demandas este estudo teve como objetivo implementar um centro de produção de composto orgânico com o uso resíduos sólidos orgânicos (RSO) e disponibilizá-lo a uma horta comunitária, localizada no município de Cachoeira do Sul/RS, e tendo como metodologia o emprego da tecnologia de compostagem pelo sistema de leiras. Os RSO utilizados formam obtidos na horta instalada e nas residências das 20 famílias, totalizando 60 pessoas, que realizavam o manejo e consomem os produtos da horta comunitária. O estudo foi realizado no período de 01 abril de 2022 até 29 de novembro de 2022, e resultou na coleta de 348 kg de RSO e transformado 170 kg de composto orgânico. Estes resultados de produção do composto orgânico com potencial biofertilizante, também beneficiou a comunidade local com a coleta e destinação final adequada de RSO, além de disponibilizar a sociedade um fertilizante sem prejuízo ambiental, o que também permitiu a avaliação e projeção de custos do destino atual e final dos RSO, o que permitirá ao município de Cachoeira do Sul avaliar a possibilidade de implementar outros centros de compostagem em demais áreas.
Nesting Technique : It’s Effects on Preterm Cardiorespiratory Indicators
Authors: Shamkhi Haneen Abdul-Hussein , Shawq Adraa Hussein 
Published: October 2024

Background: Premature infant born with immature body system, their organs are not ready for extra uterine life, and they are unable to deal with external stress, which could alter body functions such as cardio-respiratory function. In addition, poor muscle tone increases the chance of developing an abnormal posture. To reduce this instability, applying developmental care such as nesting is vital to promote cardio-respiratory stability, maintain position, and reduce stress in preterm.
Objectives: The study aims to assess the impact of the nesting technique on preterm cardio-respiratory parameters in various positions (supine, prone, and right lateral).
Methodology: The research used randomized controlled trial design. By simple random sampling technique was choose 60 preterm infant, and they were divided into four groups equally (supine, prone, right lateral, and control group). The cardio-respiratory parameters (heart rate, respiratory rate, and oxygen saturation) were measured before and after applying the nesting and positioning techniques for 3 consecutive days. Data were analyzed by Statistical Package for Social Sciences (SPSS) program.
Results: The study results showed a significant difference in the mean score of cardio-respiratory after three days of nesting technique with lateral, supine, and prone position groups at p= 0.000. While the control group showed there was non-significant change in the mean score of HR, RR, and Spo2 at p > 0.05.
Conclusion: The Study concluded that the nesting technique was effective for stabilizing the cardio-respiratory parameters of preterm infants. The authors recommended routinely using nesting with the ideal position technique in preterm as a part of developmental care at the neonatal intensive care unit
Comparative Analysis on Safe Traditional Practices by Iraqi and Philippine Mothers in the Treatment of Newborn Health Problems: A Narrative Review
Authors: Ghafel Hawraa Hussein , Villarino Resti Tito H. 
Published: October 2024

Background: Traditional practices are usually derived from the relationships with the environment and human attitude towards nature. Various societies, especially in developing nations, have unsafe traditional practices for newborns, traditionally associated with poverty, low educational levels, religiosity, culture, and by word of mouth intergenerationally handed over advice by elders in the families.
Objectives: This narrative review points out the prevalence, cultural significance, and health implications of unsafe traditional newborn care practices in Iraq and the Philippines.
Methods: Literature was searched in PubMed, Scopus, Web of Science, CINAHL, and Google. All iterations searched literature among mothers whose neonates were at issues, favoring traditional techniques rather than modern medicine—a total of 51 articles to be reviewed from articles published from 2019 to June 2024.
Results: The following ten critical themes of unsafe practices were identified: care of the umbilical cord, feeding, traditional healing, bathing rituals, circumcision, swaddling, and treatments for gastrointestinal, respiratory, and eye problems, besides newborn isolation and practices of exposure. All these practices are comparatively prevalent in the rural setup and are ingrained in the fabric of cultural beliefs and traditions. These could include exposure to infection, complications, developmental delays, and neonatal mortality. The practices have also been associated with the perpetuation of poverty, low literacy levels, and cultural backgrounds.
Conclusion: This review indicates that interventions towards these problems have to be multi-faceted. Multiple dimensions can be integrated into evidence-based interventions to improve education and economic opportunities, engagement with religious and cultural leaders, health system strengthening, fomenting research, and community engagement to develop culturally relevant solutions linking tradition and evidence-based care to improve newborn health outcomes.
Development and Validation of a Workflow Instrument to Evaluate the Success of Electronic Health Records Implementation from a Nursing Perspective: An Exploratory and Descriptive Study
Authors: Jaber Mohammad J. , Al-Bashaireh Ahmad M. , Kouri Osama , Aldiqs Mohannad A. , Alqudah Ola M. , Khraisat Omar M. , Bindahmsh Alanoud A. , Alshodukhi Abeer M. , Almutairi Amer O. , Hakeem Nevin A. 
Published: October 2024

ABSTRACT

Introduction
Electronic medical records (EMR) have been recognized as practical tools for the improvement of the quality and safety of healthcare despite their occasional use in hospitals worldwide. Epic is an integrated software suite with functionality ranging from patient administration through systems for healthcare providers to billing systems, integration to the primary health sector, and a facility for granting patients access to their data. The implementation process is complicated; creating effective methods requires understanding users’ attitudes about these information technologies. This study aimed to develop and validate a questionnaire that measures the efficacy of using workflow during the EMR (Epic) implementation. Furthermore, it describes the nurses’ views on the use of quality and satisfaction of workflow.

Methods
Following a literature review, an initial pool of 57 items was generated based on the following three primary constructs: use, quality, and user satisfaction with the tool’s workflow. Internal consistency reliability was assessed by calculating Cronbach’s alpha and correlation coefficients for construct validity.

Results
The final scale comprised 53 items corresponding to the following five distinct factors: use of workflow, information quality, service quality, use of EMR, and user satisfaction and the influence of workflow on clinical care. The full scale was assessed, and Cronbach’s alpha of 0.95 was found. The construct validity was assessed using the Kaiser-Meyer-Olkin measure of sampling adequacy and Bartlett’s Test of Sphericity (0.976). Construct validity was tested twice using Exploratory Factor Analysis-Principal Component Analysis.

Conclusion
The use of workflow, quality of information, quality of service, use of EMR, and user satisfaction scale have good reliability and validity and can be used to implement technology in healthcare.
HUBUNGAN KECERDASAN EMOSIONAL DENGAN KEMAMPUAN LITERASI MATEMATIKA SISWA
Authors: Ni Made Hermayanti  , Gusti Ayu Made Arna Putri  , I Putu Ade Andre Payadnya  
Published: October 2024

Penelitian ini mengkaji mengenai tingkat kecerdasan emosional dan kemampuan literasi matematika siswa kelas VIII C di SMP N 14 Denpasar. Pengkajian tersebut dilakukan berdasarkan data yang telah dikumpulkan dengan teknik pengumpulan data berupa observasi, pemberian angket (kuesioner), pemberian tes uraian, serta dokumentasi. Keadaan di lapangan menunjukan kebanyakan siswa masih memiliki kemampuan yang rendah dalam menyelesaikan soal atematika kompleks. Dari keadaan di lapangan tersebut, maka penelitian ini bertujuan untuk mengetahui bagaimana hubungan antara kecerdasan emosional dengan kemampuan literasi matematika siswa. Pendekatan kuantitatif digunakan pada penelitian ini dengan jenis penelitian ex-postfacto. Adapun desain penelitiannya menggunakan causal comparative research dengan teknik purpossive sampling. Populasi dari penelitian ini adalah siswa kelas VIII di SMP N 14 Denpasar dengan sampel yang diambil yaitu siswa kelas VIII C di sekolah tersebut. Hasil analisis deskriptif menunjukan baik tingkat kecerdasan emosional maupun kemampuan literasi matematika siswa kelas VIII C di SMP N 14 Denpasar berada pada kategori sedang. Dengan hasil analisis inferensial menunjukan terdapat hubungan kecerdasan emosional dengan kemampuan literasi matematika siswa kelas VIII C di SMP N 14 Denpasar.
Perspectivas Docentes sobre la Evaluación Según el Modelo Educativo de Preescolar 2017
Authors: Ramírez Pérez Sergio Edilberto 
Published: October 2024

El presente trabajo describe las creencias docentes sobre la evaluación según el nuevo modelo educativo de preescolar. El propósito del estudio fue identificar las creencias de los docentes de preescolar acerca de la evaluación de los aprendizajes en el aula de acuerdo con el nuevo modelo educativo de preescolar en México, respondiendo a la pregunta de investigación ¿Cuáles son las creencias de los docentes de preescolar acerca de la evaluación de los aprendizajes en el aula de acuerdo con el nuevo modelo educativo de preescolar? La investigación se llevó a cabo bajo el paradigma cualitativo, empleando el diseño de estudio de caso. Se entrevistó a los docentes con licenciatura en Educación Preescolar y licenciatura en Educación Física de un jardín de niños mediante una entrevista semiestructurada conformada. Como resultado se obtuvo que las creencias de los docentes acerca de la evaluación educativa son diversas. Los docentes no identifican los tipos de evaluación educativa que existen y continúan empleando términos de programas educativos anteriores, así mismo al indagar acerca de la definición que tiene ellos de la evaluación educativa en general se encontró que hay docentes que se centran en conocer los avances de los alumnos, los que se enfocan en los conocimientos adquiridos para continuar avanzando y los que la usan para verificar lo aprendido durante una sesión. Los docentes demuestran tener conocimientos acerca de la evaluación educativa, pero no están en función de los requerimientos del programa de estudios actual. Al sondear acerca del modelo ideal de evaluación los docentes manifestaron una amplia gama de supuestos ideales donde dejaron ver la seriedad e interés que le asignan.
Batik Scan: Empowering Cultural Heritage Knowledge Through Mobile Innovation
Authors: Ahmad Mazida , Barudin Nur Aqilah , Omar Mazni , Yasin Azman , Ibrahim Huda , Krisdiawan Rio Andriyat , Sugiharto Tito , Supriyadi Sugeng , Lesmana Iwan 
Published: October 2024

Batik Malaysia, a type of textile art known as Batik originated in Malaysia, especially in the east coast states; Kelantan, Terengganu, and Pahang. The Batik mostly uses features flower motifs and designs instead of living creatures, such as humans and animals. Even though Malaysian wear it every day, they do not have sufficient knowledge about Batik. Besides, there is a limited number of platforms where Malaysian can get access to the information of Batik in the easiest and fastest way. Hence, this study aims to design and develop a mobile app for batik identification by only capturing the motifs and designs using a camera feature. The app, called as batik scanner, provides quick information related to Batik Malaysia. This app is especially suitable for adults, batik lovers, and new learners. The advances in digital technology play an important role in helping people to identify the type of Batik Malaysia. It also can reduce the effort of new learners and Batik lovers to find out information of Batik. To ensure that users will have a good mobile user experience, an understanding of the importance and limitation of the mobile app features and behaviour is necessary. Therefore, the design thinking approach was applied as the development methodology as it provides a solution-based approach to solving problems. User requirements were gathered through interviews followed by defining the core problems. Next, innovative solutions were identified using the brainstorm technique and mind mapping. Once the batik scanner prototype was completed, user testing was carried out with the targeted respondents to refine the solution. An online form also was distributed to evaluate the usability of the prototype. The results of the evaluation indicate that the Batik Scanner need to improve some of the interface designs. The respondents were also satisfied with the functions of managing the information in the Batik Scanner. The study contributes toward understanding users’ needs in creating the solution to the identified problems. It may serve as a guideline for other developers and researchers to develop similar apps or to enhance the features of Batik Scanner to become better and easier for users.
Prophylactic antibiotic therapy during endovascular closure of patent foramen ovale: a clinical study
Authors: Tereshchenko A. S. , Merkulov E. V. , Azimova M. R. , Grishin N. S. , Sivakova O. A. 
Published: October 2024

Purpose of the study. To study the effect of prophylactic antibiotic therapy on the course of the postoperative period in patients undergoing endovascular closure of patent foramen ovale (PFO). Material and methods. The study included 276 patients who were submitted to endovascular closure of PFO. The follow-up duration was 12 months. Depending on prophylactic antibiotic therapy the patients were divided into 2 groups - those who did not receive and those who received prophylactic antibiotic therapy (115 and 161 patients, respectively). Results. Cephalosporins were prescribed to patients as prophylactic antibiotic therapy, and in the presence of allergic reactions they were replaced by glycopeptides. In total, patients received 1 to 2 drugs per prophylaxis course. Postoperative complications were present in 37 (22.98 %) patients who received prophylactic antibiotic therapy and 12 (10.43 %) who did not receive prophylaxis (p = 0.007). Access site complications were the most identified, but they were not inflammatory in nature. An elevation of body temperature over 37.1 °C was observed in 19 (11.80 %) patients receiving prophylactic antibiotic therapy compared to 5 (4.35 %) who did not receive prophylaxis (p = 0.03). Meanwhile, marked leukocytosis was absent in both groups. The duration of body temperature elevation did not differ between the groups. They also had a longer duration of hospitalization, 7.00 [6.00; 8.00] bed days, compared to 6.00 [4.00; 7.00]. Discussion. It has been suggested that hyperthermia may be associated with prophylactic administration of antibacterial drugs causing death of persistent microflora. In patients who did not receive prophylactic antibiotic therapy, their concentration was lower, which did not lead to an increase in body temperature. Since there are no indications on the necessity of prophylactic antibiotic therapy before endovascular closure of PFO in clinical recommendations, it is suggested to evaluate its appropriateness for each patient individually.