bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Organic Phosphorus Contributions for Predicting Soybean Response to Fertilization
Authors: Appelhans Stefania C. , Melchiori Ricardo J. , Barbagelata Pedro A. , Novelli Leonardo E. 
Published: October 2016

Core Ideas
The contribution of P indices that include organic P to predict soybean P fertilization response was evaluated.
Bioavailable P and MA‐P indices correlated with soybean relative yield.
MA‐P index correlated with labile organic P fractions.
MA‐P index improved the accuracy to predict soybean yield response to applied P.
The use of organic P fraction in soil test P for soybean [Glycine max (L.) Merr.] under no‐till could improve the accuracy to predict crops response to P fertilization. The research objectives were to assess the contribution and accuracy of P indices that include organic‐P fractions to predict soybean yield response to P fertilization in comparison with Bray soil test P (BP). The study included P fertilization experiments conducted in the Pampas Region of Argentina during three growing seasons. We selected sites considering Cate and Nelson quadrants (I, II, III), established by a critical BP concentration of 9 mg P kg−1 and a relative soybean yield (RY) of 85%. Bray P, bioavailable (BioP), particulate (POM‐P), and mineral‐associated (MA‐P) soil P fractions were determined in soil samples from the 0‐ to 5‐cm and 0‐ to 20‐cm depths. Bray P varied from 4.8 to 31 mg P kg−1 and BioP from 8 to 29 mg P kg−1 at the 0‐ to 20‐cm depth. Phosphorus content in POM ranged from 19 to 171 mg P kg−1, and MA‐P ranged from 208 to 446 mg P kg−1. Bioavailable P and MA‐P correlated with RY, whereas POM‐P did not. Bioavailable P performed similarly to BP test to predict soybean yield response to P fertilization. Phosphorus content in MA fraction reduced Cate–Nelson classification errors, improving the accuracy to predict soybean yield response to applied P in comparison with BP test. Sampling at the 0‐ to 5‐cm depth did not improve soil test P performance compared with the 0‐ to 20‐cm depth.
DEEP CHANDRA OBSERVATIONS OF THE COMPACT STARBURST GALAXY HENIZE 2–10: X-RAYS FROM THE MASSIVE BLACK HOLE
Authors: Reines Amy E. , Reynolds Mark T. , Miller Jon M. , Sivakoff Gregory R. , Greene Jenny E. , Hickox Ryan C. , Johnson Kelsey E. 
Published: October 2016

ABSTRACT
We present follow-up X-ray observations of the candidate massive black hole (BH) in the nucleus of the low-mass, compact starburst galaxy Henize 2–10. Using new high-resolution observations from the Chandra X-ray Observatory totaling 200 ks in duration, as well as archival Chandra observations from 2001, we demonstrate the presence of a previously unidentified X-ray point source that is spatially coincident with the known nuclear radio source in Henize 2–10 (i.e., the massive BH). We show that the hard X-ray emission previously identified in the 2001 observation is dominated by a source that is distinct from the nucleus, with the properties expected for a high-mass X-ray binary. The X-ray luminosity of the nuclear source suggests the massive BH is radiating significantly below its Eddington limit (∼10

), and the soft spectrum resembles other weakly accreting massive BHs including Sagittarius A*. Analysis of the X-ray light curve of the nucleus reveals the tentative detection of a ∼9 hr periodicity, although additional observations are required to confirm this result. Our study highlights the need for sensitive high-resolution X-ray observations to probe low-level accretion, which is the dominant mode of BH activity throughout the universe.
KIC 8462852 FADED THROUGHOUT THE KEPLER MISSION
Authors: Montet Benjamin T. , Simon Joshua D. 
Published: October 2016

ABSTRACT
KIC 8462852 is a superficially ordinary main sequence F star for which Kepler detected an unusual series of brief dimming events. We obtain accurate relative photometry of KIC 8462852 from the Kepler full-frame images, finding that the brightness of KIC 8462852 monotonically decreased over the four years it was observed by Kepler. Over the first ∼1000 days KIC 8462852 faded approximately linearly at a rate of 0.341 ± 0.041% yr−1, for a total decline of 0.9%. KIC 8462852 then dimmed much more rapidly in the next ∼200 days, with its flux dropping by more than 2%. For the final ∼200 days of Kepler photometry the magnitude remained approximately constant, although the data are also consistent with the decline rate measured for the first 2.7 years. Of a sample of 193 nearby comparison stars and 355 stars with similar stellar parameters, none exhibit the rapid decline by >2% or the cumulative fading by 3% of KIC 8462852. Moreover, of these comparison stars, only one changes brightness as quickly as the 0.341% yr−1 measured for KIC 8462852 during the first three years of the Kepler mission. We examine whether the rapid decline could be caused by a cloud of transiting circumstellar material, finding that while such a cloud could evade detection in submillimeter observations, the transit ingress and duration cannot be explained by a simple cloud model. Moreover, this model cannot account for the observed longer-term dimming. No known or proposed stellar phenomena can fully explain all aspects of the observed light curve.
Menin Plays a Critical Role in the Regulation of the Antigen-Specific CD8+ T Cell Response upon Listeria Infection
Authors: Yamada Takeshi , Kanoh Makoto , Nabe Shogo , Yasuoka Toshiaki , Suzuki Junpei , Matsumoto Akira , Kuwahara Makoto , Maruyama Saho , Fujimoto Takuya , Sakisuka Ryo , Yasukawa Masaki , Yamashita Masakatsu 
Published: October 2016

Abstract
Menin, a tumor suppressor protein, is encoded by the MEN1 gene in humans. Certain germinal mutations of MEN1 induce an autosomal-dominant syndrome that is characterized by concurrent parathyroid adenomas and several other tumor types. Although menin is also expressed in hematopoietic lineages, its role in CD8+ T cells remains unclear. We generated Meninflox/flox CD4-Cre (Menin-KO) mice by crossing Meninflox/flox mice with CD4-Cre transgenic (Tg) mice to determine the role of menin in CD8+ T cells. Wild-type (WT) and Menin-KO mice were infected with Listeria monocytogenes expressing OVA to analyze the immune response of Ag-specific CD8+ T cells. Menin deficiency resulted in an impaired primary immune response by CD8+ T cells. On day 7, there were fewer Menin-KO OVA-specific CD8+ T cells compared with WT cells. Next, we adoptively transferred WT and Menin-KO OT-1 Tg CD8+ T cells into congenic recipient mice and infected them with L. monocytogenes expressing OVA to determine the CD8+ T cell–intrinsic effect. Menin-KO OT-1 Tg CD8+ T cells were outcompeted by the WT cells upon infection. Increased expression of Blimp-1 and T-bet, cell cycle inhibitors, and proapoptotic genes was observed in the Menin-KO OT-1 Tg CD8+ T cells upon infection. These data suggest that menin inhibits differentiation into terminal effectors and positively controls proliferation and survival of Ag-specific CD8+ T cells that are activated upon infection. Collectively, our study uncovered an important role for menin in the immune response of CD8+ T cells to infection.
Germinal Center Hypoxia Potentiates Immunoglobulin Class Switch Recombination
Authors: Abbott Robert K. , Thayer Molly , Labuda Jasmine , Silva Murillo , Philbrook Phaethon , Cain Derek W. , Kojima Hidefumi , Hatfield Stephen , Sethumadhavan Shalini , Ohta Akio , Reinherz Ellis L. , Kelsoe Garnett , Sitkovsky Michail 
Published: October 2016

Abstract
Germinal centers (GCs) are anatomic sites where B cells undergo secondary diversification to produce high-affinity, class-switched Abs. We hypothesized that proliferating B cells in GCs create a hypoxic microenvironment that governs their further differentiation. Using molecular markers, we found GCs to be predominantly hypoxic. Compared to normoxia (21% O2), hypoxic culture conditions (1% O2) in vitro accelerated class switching and plasma cell formation and enhanced expression of GL-7 on B and CD4+ T cells. Reversal of GC hypoxia in vivo by breathing 60% O2 during immunization resulted in reduced frequencies of GC B cells, T follicular helper cells, and plasmacytes, as well as lower expression of ICOS on T follicular helper cells. Importantly, this reversal of GC hypoxia decreased Ag-specific serum IgG1 and reduced the frequency of IgG1+ B cells within the Ag-specific GC. Taken together, these observations reveal a critical role for hypoxia in GC B cell differentiation.
Glacier change and glacial lake outburst flood risk in the Bolivian Andes
Authors: Cook Simon J. , Kougkoulos Ioannis , Edwards Laura A. , Dortch Jason , Hoffmann Dirk 
Published: October 2016

Abstract. Glaciers of the Bolivian Andes represent an important water resource for Andean cities and mountain communities, yet relatively little work has assessed changes in their extent over recent decades. In many mountain regions, glacier recession has been accompanied by the development of proglacial lakes, which can pose a glacial lake outburst flood (GLOF) hazard. However, no studies have assessed the development of such lakes in Bolivia despite recent GLOF incidents here. Our mapping from satellite imagery reveals an overall areal shrinkage of 228.1 ± 22.8 km2 (43.1 %) across the Bolivian Cordillera Oriental between 1986 and 2014. Shrinkage was greatest in the Tres Cruces region (47.3 %), followed by the Cordillera Apolobamba (43.1 %) and Cordillera Real (41.9 %). A growing number of proglacial lakes have developed as glaciers have receded, in accordance with trends in most other deglaciating mountain ranges, although the number of ice-contact lakes has decreased. The reasons for this are unclear, but the pattern of lake change has varied significantly throughout the study period, suggesting that monitoring of future lake development is required as ice continues to recede. Ultimately, we use our 2014 database of proglacial lakes to assess GLOF risk across the Bolivian Andes. We identify 25 lakes that pose a potential GLOF threat to downstream communities and infrastructure. We suggest that further studies of potential GLOF impacts are urgently required.
Bioanalytical Method Validation for Dronedarone and Duloxetine in Blood Serum
Authors: Chadha Renu , Bali Alka , Bansal Gulshan 
Published: October 2016

Abstract
The present work relates to the development and validation of reversed-phase HPLC–UV-photodiode array methods for the estimation of two drugs in blood serum: dronedarone hydrochloride (DDN), a class III antiarrhythmic drug, and duloxetine hydrochloride (DLX), an antidepressant. Chromatographic analysis of DLX was carried out on a Nucleodur C18 column (250 × 4.6 mm, 5 μm) using ammonium acetate buffer (32 mM, pH 5.5) and acetonitrile (40 + 60, v/v; flow rate of 1.0 mL/min; detection wavelength of 290 nm) as the mobile phase. A Waters XTerra C18 column (250 × 4.6 mm, 5 μm) was used for the chromatographic analysis ofDDN using an acetonitrile–ammonium formate buffer (20 mM, pH 3.0, with formic acid; 45 + 55, v/v; flow rate 1.0 mL/min) as the mobile phase. Pentazocine and bupropion HCl were used as the internal reference standards for DLX and DDN, respectively. Excellent linearity was observed for DLX (r2 = 0.9996; concentration range 0.2–10.0 μg/mL) and DDN (r2 = 0.9997; concn. range 2.0–50.0 μg/mL). The LODs for DLX and DDN were 0.022 and 0.78 μg/mL, respectively, and the LOQs 0.066 and 2.4 μg/mL, respectively.
Improving Adequacy of Small Biopsy and Fine-Needle Aspiration Specimens for Molecular Testing by Next-Generation Sequencing in Patients With Lung Cancer: A Quality Improvement Study at Dartmouth-Hitchcock Medical Center
Authors: Padmanabhan Vijayalakshmi , Steinmetz Heather B. , Rizzo Elizabeth J. , Erskine Amber J. , Fairbank Tamara L. , de Abreu Francine B. , Tsongalis Gregory J. , Tafe Laura J. 
Published: October 2016

Context.—
At our medical center, cytopathologists perform rapid on-site evaluation for specimen adequacy of fine-needle aspiration and touch imprint of needle core biopsy lung cancer samples. Two years ago the molecular diagnostics laboratory at our institution changed to next-generation sequencing using the Ion Torrent PGM and the 50-gene AmpliSeq Cancer Hotspot Panel v2 for analyzing mutations in a 50-gene cancer hot spot panel. This was associated with a dramatic fall in adequacy rate (68%).

Objective.—
To improve the adequacy rate to at least 90% for molecular testing using next-generation sequencing for all specimens collected by rapid on-site evaluation by the cytology laboratory.

Design.—
After baseline data on adequacy rate of cytology specimens with rapid on-site evaluation for molecular testing had been collected, 2 changes were implemented. Change 1 concentrated all the material in one block but did not produce desired results; change 2, in addition, faced the block only once with unstained slides cut up front for molecular testing. Data were collected in an Excel spreadsheet and adequacy rate was assessed.

Results.—
Following process changes 1 and 2 we reached our goal of at least 90% adequacy rate for molecular testing by next-generation sequencing on samples collected by rapid on-site evaluation including computed tomography–guided needle core biopsies (94%; 17 of 18) and fine-needle aspiration samples (94%; 30 of 32).

Conclusion.—
This study focused on factors that are controllable in a pathology department and on maximizing use of scant tissue. Optimizing the adequacy of the specimen available for molecular tests avoids the need for a second procedure to obtain additional tissue.
Laboratory Diagnosis of Zika Virus Infection
Authors: Landry Marie Louise , St. George Kirsten 
Published: October 2016

Context.—The rapid and accurate diagnosis of Zika virus infection is an international priority.
Objective.—To review current recommendations, methods, limitations, and priorities for Zika virus testing.
Data Sources.—Sources include published literature, public health recommendations, laboratory procedures, and testing experience.
Conclusions.—Until recently, the laboratory diagnosis of Zika infection was confined to public health or research laboratories that prepared their own reagents, and test capacity has been limited. Furthermore, Zika cross-reacts serologically with other flaviviruses, such as dengue, West Nile, and yellow fever. Current or past infection, or even vaccination with another flavivirus, will often cause false-positive or uninterpretable Zika serology results. Detection of viral RNA during acute infection using nucleic acid amplification tests provides more specific results, and a number of commercial nucleic acid amplification tests have received emergency use authorization. In addition to serum, testing of whole blood and urine is recommended because of the higher vial loads and longer duration of shedding. However, nucleic acid amplification testing has limited utility because many patients are asymptomatic or present for testing after the brief period of Zika shedding has passed. Thus, the greatest need and most difficult challenge is development of accurate antibody tests for the diagnosis of recent Zika infection. Research is urgently needed to identify Zika virus epitopes that do not cross-react with other flavivirus antigens. New information is emerging at a rapid pace and, with ongoing public-private and international collaborations and government support, it is hoped that rapid progress will be made in developing robust and widely applicable diagnostic tools.
Pour une écoute poétique de la matière sonore : Brume de Dieu de Claude Régy
Authors: Dodet Cyrielle 
Published: October 2016

Cet article analyse l’écoute complexe dans Brume de Dieu, créé par le metteur en scène français Claude Régy en 2010, d’après le roman Les oiseaux de Tarjei Vesaas. À l’instar de l’acuité auditive extrême du protagoniste Mattis, le spectateur est amené à développer une écoute poétique eu égard à la diction singulière du comédien, à la conception sonore de Philippe Cachia, aux perceptions sensorielles rendues incertaines ainsi qu’à la qualité du silence façonné par Régy. Rythme et signifiance permettent de créer des points d’écoute dans cette puissante matière sonore, tout en les mettant à l’épreuve.
Reinforcement of the Flexor Tendon Repair Using Human Amniotic Membrane
Authors: Dogramaci Yunus , Duman Ibrahim Gökhan 
Published: October 2016

Background:Human amniotic membrane is used to prevent peritendinous adhesions after tendon injuries. This study compares the mechanical properties of modified Kessler repairs and modified Kessler repairs strengthened using multiple layers of human amniotic membrane.Methods:Twenty flexor digitorum profundus tendons of sheep forelimbs were sutured by the two-strand modified Kessler technique (group A) and by the two-strand modified Kessler repair reinforced with multiple layers of human amniotic membrane (group B). To assess the mechanical performance of the repairs, tendons were subjected to a linear noncyclic load-to-failure test using a material testing machine. Outcome measures included ultimate forces and the mode of failure.Results:The mean ± SD value of the failure strength was 34.6 ± 1.64 N for group A and 50.6 ± 5.60 N for group B. The reinforced repair provided a significantly higher ultimate load compared with the nonreinforced group (P &lt; .001). All of the specimens failed due to suture breakage at the repair site.Conclusions:The results of this study show that the modified Kessler repair can be reinforced effectively with human amniotic membrane.
First Metatarsal Head and Medial Eminence Widths with and Without Hallux Valgus
Authors: Lenz Robin C. , Nagesh Darshan , Park Hannah K. , Grady John 
Published: October 2016

Background:
Resection of the medial eminence in hallux valgus surgery is common. True hypertrophy of the medial eminence in hallux valgus is debated. No studies have compared metatarsal head width in patients with hallux valgus and control patients.

Methods:
We reviewed 43 radiographs with hallux valgus and 27 without hallux valgus. We measured medial eminence width, first metatarsal head width, and first metatarsal shaft width in patients with and without radiographic hallux valgus.

Results:
Medial eminence width was 1.12 mm larger in patients with hallux valgus (P &lt; .0001). Metatarsal head width was 2.81 mm larger in patients with hallux valgus (P &lt; .001). Metatarsal shaft width showed no significant difference (P = .63).

Conclusions:
Metatarsal head width and medial eminence width are significantly larger on anteroposterior weightbearing radiographs in patients with hallux valgus. However, frontal plane rotation of the first metatarsal likely accounts for this difference.
A pilot study examining the effects of low-volume high-intensity interval training and continuous low to moderate intensity training on quality of life, functional capacity and cardiovascular risk factors in cancer survivors
Authors: Toohey Kellie , Pumpa Kate L. , Arnolda Leonard , Cooke Julie , Yip Desmond , Craft Paul S. , Semple Stuart 
Published: October 2016

PurposeThe aim of this study was to evaluate the effects of low-volume high-intensity interval training and continuous low to moderate intensity training on quality of life, functional capacity and cardiovascular disease risk factors in cancer survivors.MethodsCancer survivors within 24 months post-diagnosis were randomly assigned into the low-volume high-intensity interval training group (n = 8) or the continuous low to moderate intensity training group (n = 8) group for 36 sessions (12 weeks) of supervised exercise. The low-volume high-intensity interval training (LVHIIT) group performed 7 × 30 s intervals (≥85% maximal heart rate) and the continuous low to moderate intensity training (CLMIT) group performed continuous aerobic training for 20 min (≤55% maximal heart rate) on a stationary bike or treadmill.ResultsSignificant improvements (time) were observed for 13 of the 23 dependent variables (ES 0.05–0.61,p ≤ 0.05). An interaction effect was observed for six minute walk test (18.53% [32.43–4.63] ES 0.50,p ≤ 0.01) with the LVHIIT group demonstrating greater improvements.ConclusionThese preliminary findings suggest that both interventions can induce improvements in quality of life, functional capacity and selected cardiovascular disease risk factors. The LVHIIT program was well tolerated by the participants and our results suggest that LVHIIT is the preferred modality to improve fitness (6MWT); it remains to be seen which intervention elicits the most clinically relevant outcomes for patients. A larger sample size with a control group is required to confirm the significance of these findings.
Origin of intraplate volcanism in northeast China from Love wave constraints
Authors: Fu Yuanyuan V. , Gao Yuan , Li Aibing , Li Lun , Shi Yutao , Zhang Yi 
Published: October 2016

AbstractThe tectonic source for widespread volcanism in northeast China has not been completely understood. We develop a 3‐D SH velocity model in NE China that provides new constraints to the origin of the volcanism. The 3‐D model is constructed from fundamental mode Love waves at the periods of 20–125 s recorded at 269 broadband seismic stations. The Changbai Mountain is characterized by a significant low velocity in the lower crust and uppermost mantle, which probably results from mantle upwelling due to the subduction of the Pacific plate. A fast and thin mantle lid of ~75 km is present beneath the Songliao Basin, indicating lithosphere extension from back‐arc rifting. The slow velocity in the middle and fast velocities in the south and north at 75–115 km depths in the Songliao Basin suggest complex mantle flow with upwelling and downwelling. Unlike the other volcanic fields (Changbaishan volcano, Jingpohu volcano, and Abaga volcano), the Halaha volcano has high velocity in the lower crust and upper mantle, implying a limited melt supply from mantle source recently. The subduction‐induced upwelling leads to complicated small‐scale mantle convection, which is responsible for the intraplate magmatism in northeast China.
Ice nucleation activity of agricultural soil dust aerosols from Mongolia, Argentina, and Germany
Authors: Steinke I. , Funk R. , Busse J. , Iturri A. , Kirchen S. , Leue M. , Möhler O. , Schwartz T. , Schnaiter M. , Sierau B. , Toprak E. , Ullrich R. , Ulrich A. , Hoose C. , Leisner T. 
Published: October 2016

AbstractSoil dust particles emitted from agricultural areas contain considerable mass fractions of organic material. Also, soil dust particles may act as carriers for potentially ice‐active biological particles. In this work, we present ice nucleation experiments conducted in the Aerosol Interaction and Dynamics in the Atmosphere (AIDA) cloud chamber. We investigated the ice nucleation efficiency of four types of soil dust from different regions of the world. The results are expressed as ice nucleation active surface site (INAS) densities and presented for the immersion freezing and the deposition nucleation mode. For immersion freezing occurring at 254 K, samples from Argentina, China, and Germany show ice nucleation efficiencies which are by a factor of 10 higher than desert dusts. On average, the difference in ice nucleation efficiencies between agricultural and desert dusts becomes significantly smaller at temperatures below 247 K. In the deposition mode the soil dusts showed higher ice nucleation activity than Arizona Test Dust over a temperature range between 232 and 248 K and humidities RHice up to 125%. INAS densities varied between 109 and 1011 m−2 for these thermodynamic conditions. For one soil dust sample (Argentinian Soil), the effect of treatments with heat was investigated. Heat treatments (383 K) did not affect the ice nucleation efficiency observed at 249 K. This finding presumably excludes proteinaceous ice‐nucleating entities as the only source of the increased ice nucleation efficiency.