bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, characterization, and biological and anticancer studies of mixed ligand complexes with Schiff base and 2,2′‐bipyridine
Authors: Omar M.M. , Abd El‐Halim Hanan F. , Khalil Eman A.M. 
Published: February 2017

New mixed ligand complexes of transition metals were synthesized from a Schiff base (L1) obtained by the condensation reaction of oxamide and furfural as primary ligand and 2,2′‐bipyridine (L2) as secondary ligand. The ligands and their metal complexes were studied using various spectroscopic methods. Also thermal analyses were conducted. The mixed ligand complexes were found to have formulae [M(L1)(L2)]Clm⋅nH2O (M = Cr(III) and Fe(III): m = 3, n = 0; M = Cu(II) and Cd(II): m = 2, n = 1; M = Mn(II), Co(II), Ni(II) and Zn(II): m = 2, n = 0). The resultant data revealed that the metal complexes have octahedral structure. Also, the mixed ligand complexes are electrolytic. The biological and anticancer activities of the new compounds were tested against breast cancer (MCF‐7) and colon cancer (HCT‐116) cell lines. The results showed high activity for the synthesized compounds.
Preparation of microcrystalline cellulose from rice straw under microwave irradiation
Authors: Fan Guo‐Zhi , Wang Yue‐Xin , Song Guang‐Sen , Yan Jun‐Tao , Li Jian‐Fen 
Published: February 2017

ABSTRACTCrude cellulose was isolated from rice straw and then converted into microcrystalline cellulose (MCC) via partial hydrolysis. Both the isolation and partial hydrolysis were carried out under microwave. Rice straw was successively pretreated with alkaline and acid solutions before hydrolysis. Lignin and silica were almost removed but partial removal of hemicellulose in dilute alkali solution, and the residual hemicellulose was further removed in dilute acid solution. The total removal rates of hemicellulose, lignin and silica reached up 92.0%, 98.5%, and 96.8%, respectively. The cellulose content of 93.6 wt % in MCC was given by partial hydrolysis of crude cellulose isolated from rice straw. The comparison between microwave irradiation and traditional heating method was also investigated. Compared to traditional heating method, similar results were obtained while milder conditions, including shorter time and lower temperature, were required. An effective microwave‐assisted and energy‐efficient methodology using rice straw as the alternative source of cellulose fiber was developed. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44901.
The Passive Seismic Technique ‘HVSR’ as a Reconnaissance Tool for Mapping Paleo‐soils: The Case of the Pilastri Archaeological Site, Northern Italy
Authors: Abu Zeid Nasser , Corradini Erica , Bignardi Samuel , Nizzo Valentino , Santarato Giovanni 
Published: February 2017

AbstractHorizontal‐to‐vertical spectral ratio (HVSR) is a widely used geophysical technique in seismic microzonation studies. It is based on a specific analysis of seismic ambient noise. The method allows to obtain the frequency and amplitude of the resonance peaks of a layered earth with increasing acoustic impedance contrasts. The peaks can be interpreted to obtain an estimation of depth(s) of the impedance contrast horizon(s).Based on the assumption that long‐term human trampling results in sediment's stiffening, which increased both density and velocity of seismic shear waves, the HVSR method was applied to investigate the shallow subsurface of an important, Middle Bronze Age, archaeological site called ‘Pilastri Terramara’ discovered at the end of the last century.Following recent excavations, archaeologists supposed that the settlement could extend outside the initially hypothesized borders, and decided to involve geophysicists to verify the truthiness of this new hypothesis and consequently to map the possible spatial extent of the paleo‐surfaces frequented by ancient occupants. The purpose of the geophysical investigation was then to detect and possibly to map one or more anthropogenic paleo‐surfaces over a relatively large area (about 12000 m2).Unfortunately, direct evidences showed that the paleo‐surfaces were embedded in clayey sediments and laying at depths ranging between 50 and 170 cm below ground level. Furthermore, the area to be investigated is occupied by a farm with glasshouses and other buildings. These obstacles constituted a real challenge that hindered the utilization of the most commonly used geophysical methods in archaeology, i.e. ground penetration radar (GPR), magnetometry and electrical resistivity tomography. For these reasons, we decided to use the HVSR method as a reconnaissance exploration tool, to confirm or rule out the presence of such paleo‐surfaces.Spectral peaks related to acoustic horizons provided evidences about their presence and allowed to estimate their depths as was later confirmed by a new excavation. Copyright © 2017 John Wiley & Sons, Ltd.
Slow Sampling Control of Singularly Perturbed Systems Subject to Actuator Saturation and L2 Disturbance
Authors: Ma Lei , Cai Chenxiao , Ma Xiaoping 
Published: February 2017

AbstractThis paper is concerned with the slow sampling control problem for singularly perturbed systems with actuator saturation and L2 disturbance. Convex hull is utilized to deal with the actuator saturation. A sufficient condition is proposed such that the boundedness of all the state trajectories will be guaranteed for any singular perturbation parameter less than or equal to a predefined upper bound in the presence of L2 disturbance. Then, two convex optimization problems are formulated to estimate the capability of disturbance tolerance/rejection based on this condition. Finally, an example is presented to demonstrate the effectiveness of the main results of this paper.
V‐type near‐Earth asteroids: Dynamics, close encounters and impacts with terrestrial planets
Authors: Galiazzo M. A. , Silber E. A. , Bancelin D. 
Published: February 2017

BackgroundAsteroids colliding with planets vary in composition and taxonomical type. Among Near‐Earth Asteroids (NEAs) are the V‐types, basaltic asteroids that are classified via spectroscopic observations.Materials and Methodswe performed numerical simulations and statistical analysis of close encounters and impacts between V‐type NEAs and the terrestrial planets over the next 10 Myr. We study the probability of V‐ type NEAs colliding with Earth, Mars and Venus, as well as the Moon. We perform a correlational analysis of possible craters produced by V‐type NEAs, using available catalogs for terrestrial impact craters.ResultsThe results suggest that V‐type NEAs can have many close encounters below 1 LD and even some of them impacts with all the terrestrial planets, the Earth in particular. There are four candidate craters on Earth that were likely caused by V‐type NEAs.ConclusionAt least 70% of the V‐type NEAs can have close encounters with the terrestrial planets (and 58% with the Moon) . They can collide with all the terrestrial planets. In particular for the Earth the average rate is one every ∼12 Myr. The two craters with the highest probability of being generated by an impact with a basaltic impactor are: the Strangways crater (24 km diameter) in Australia and the Nicholson crater (12.5 km diameter) in Canada.
A simple way to unify multicriteria decision analysis (MCDA) and stochastic multicriteria acceptability analysis (SMAA) using a Dirichlet distribution in benefit–risk assessment
Authors: Saint‐Hilary Gaelle , Cadour Stephanie , Robert Veronique , Gasparini Mauro 
Published: February 2017

Quantitative methodologies have been proposed to support decision making in drug development and monitoring. In particular, multicriteria decision analysis (MCDA) and stochastic multicriteria acceptability analysis (SMAA) are useful tools to assess the benefit–risk ratio of medicines according to the performances of the treatments on several criteria, accounting for the preferences of the decision makers regarding the relative importance of these criteria. However, even in its probabilistic form, MCDA requires the exact elicitations of the weights of the criteria by the decision makers, which may be difficult to achieve in practice. SMAA allows for more flexibility and can be used with unknown or partially known preferences, but it is less popular due to its increased complexity and the high degree of uncertainty in its results. In this paper, we propose a simple model as a generalization of MCDA and SMAA, by applying a Dirichlet distribution to the weights of the criteria and by making its parameters vary. This unique model permits to fit both MCDA and SMAA, and allows for a more extended exploration of the benefit–risk assessment of treatments. The precision of its results depends on the precision parameter of the Dirichlet distribution, which could be naturally interpreted as the strength of confidence of the decision makers in their elicitation of preferences.
Low nanomolar caffeic acid attenuates high glucose‐induced endothelial dysfunction in primary human umbilical‐vein endothelial cells by affecting NF‐κB and Nrf2 pathways
Authors: Fratantonio Deborah , Speciale Antonio , Canali Raffaella , Natarelli Lucia , Ferrari Daniela , Saija Antonina , Virgili Fabio , Cimino Francesco 
Published: February 2017

AbstractHyperglycemia contributes to dysregulate endothelial function associated with diabetes, leading to initiation and propagation of vascular complications and dysfunction. Caffeic acid (CA), a dietary hydroxycinnamic acid abundant in coffee, has been reported to exert antidiabetic effects in rat models. Herein, we investigated the molecular effects of physiological concentrations of CA (10 nM) against endothelial dysfunction induced by high glucose (HG) in human endothelial cells (HUVECs). HUVECs were exposed to HG 25 mM, to mimic diabetic condition, in presence of CA. Intracellular redox status (reduced glutathione, superoxide dismutase (SOD) and total antioxidant activity levels), and NF‐κB pathway were examined. We also evaluated the involvement of NF‐E2‐related factor 2 (Nrf2)/electrophile responsive element (EpRE) pathway. Our data show that CA inhibits HG‐induced nuclear translocation of NF‐κB and the downstream expression of endothelial adhesion molecule 1 and restores antioxidant levels by upregulating Nrf2/EpRE pathway. Our data suggest that CA can suppress several aspects of HG‐induced endothelial dysfunction through the modulation of intracellular redox status controlled by the transcription factor Nrf2. These findings highlight that low physiological concentration of CA achievable specifically upon food consumption are able to prevent endothelial dysfunction associated with inflammation and oxidative stress induced by high concentration of glucose. © 2016 BioFactors, 43(1):54–62, 2017
Site‐specific monitoring of N‐Glycosylation profiles of a CTLA4‐Fc‐fusion protein from the secretory pathway to the extracellular environment
Authors: Bora de Oliveira Karina , Spencer David , Barton Christopher , Agarwal Nitin 
Published: February 2017

ABSTRACTGlycosylation often plays a key role in the safety and efficacy of therapeutic proteins to patients, thus underlying the need for consistent control of this important post‐translational modification during biologics production. In this study, we profiled the site‐specific evolution of N‐glycans on a CTLA4‐Fc‐fusion protein, from the intracellular secretory pathway to the conditioned medium (CM) in fed‐batch cell culture. For this, we developed an approach that combined sub‐cellular fractionation with liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analyses. The study revealed that there was a significant amount of heterogeneity in the glycans displayed amongst the three distinct N‐glycosylation sites. Furthermore, 54–60% of the intracellular protein was characterized by Man8 and Man9 glycans on day 10, when the cell density peaks, indicative of a significant bottleneck between the endoplasmic reticulum (ER) and the cis‐Golgi. At longer culture duration, the accumulation of intracellular protein with bi‐antennary‐fucosylated GlcNAc‐terminated residues identified the formation of another bottleneck in the medial and trans‐Golgi compartments, which subsequently led to a decrease in sialylated species in the secreted protein. Glucose deprivation caused a reduction in the Man8 and Man9 glycans in favor of Man5 glycans and bi‐antennary‐fucosylated GlcNAc‐terminated residues in the organellar pool of the Fc‐fusion protein. However, transient deprivation of glucose did not lead to major differences in the glycan profile of proteins secreted into the CM. The approach developed here allows us to probe the secretory pathway and sheds light on the site‐specific intracellular processing of glycans during fed‐batch cell culture, thus serving as an initial step towards their rational control. Biotechnol. Bioeng. 2017;114: 1550–1560. © 2017 Wiley Periodicals, Inc.
A novel cholesterol/lipid delivery system for murine myeloma cell lines
Authors: Chauhan Gaurav , Schmelzer Albert E. 
Published: February 2017

Murine myeloma NS0 cells are cholesterol‐dependent auxotrophs and require externally provided cholesterol for sustained growth. Traditionally, cholesterol is provided to these cells by supplementing cell culture media with a concentrated solution of cholesterol and other water insoluble components dissolved in 200‐proof ethanol. However, the solubility of cholesterol in ethanol is limited, and for processes requiring large amounts of cholesterol, the consequential increase in added ethanol may negatively impact cell growth. Additionally, the flammability of 200‐proof ethanol may restrict the preparation scale and storage volumes at a large‐scale facility, thus resulting in a more complex preparation procedure due to safety guidelines. This study proposes 1‐propanol as an alternative solvent, which can dissolve up to 40 g L−1 of cholesterol along with other water insoluble components, as compared to ethanol, which can dissolve up to 10 g L−1 of the same. A concentrated formulation simplifies the preparation method and ameliorates the procedural and operational challenges, as well as reduces the total amount of alcohol added to a cell culture by ∼80% when compared to the ethanolic solution, to deliver the same amount of cholesterol, thereby significantly minimizing alcohol exposure to the cells and mitigating the fire hazards at a large‐scale facility. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:795–803, 2017
Salmonella effector SopD2 interferes with Rab34 function
Authors: Teo Wei Xuan , Yang Zhe , Kerr Markus Charles , Luo Lin , Guo Zhong , Alexandrov Kirill , Stow Jenny Lea , Teasdale Rohan David 
Published: February 2017

AbstractMany intracellular pathogens have evolved highly specialized mechanisms to isolate themselves from the host cell's innate immune response while still obtaining the necessary nutrients to survive. Salmonella utilizes type 3 secretion systems (T3SSs) to deliver bacterial proteins called effectors, across the encompassing Salmonella Containing vacuole (SCV) membrane, to subvert the host's membrane trafficking pathways and alter other cellular processes. The Salmonella Pathogenicity Island (SPI)‐2 effector SopD2 has recently been demonstrated to modulate multiple members of the Rab GTPase family such as Rab7, Rab8, Rab10, and Rab32 (D'Costa et al., , Cell Reports, 12:1508–18; Spano et al., , Cell Host & Microbe, 19:216–26). Here, we demonstrate the additional capacity of SopD2 to bind Rab34 and modulate its function. Our data indicate that depletion of Rab34 delays maturation of the SCV, and consequently, inhibits intracellular Salmonella enterica serotype typhimurium (S. typhimurium) growth. Interestingly, intracellular growth of the S. typhimurium lacking SopD2 was severely impaired in Rab34‐depleted cells, suggesting a compounding virulence effect. Overall this study reveals an additional member of the Rab GTPase family, Rab34, that is modulated by SopD2 and provides insight into its role in Salmonella biology.
Characteristics and outcomes of re‐do percutaneous paravalvular leak closure
Authors: Al‐Hijji Mohammed A. , Alkhouli Mohamad , Sarraf Mohammad , Zack Chad J. , Malouf Joseph F. , Nkomo Vuyisile T. , Cabalka Allison K. , Reeder Guy S. , Rihal Charanjit S. , Eleid Mackram F. 
Published: February 2017

BackgroundPercutaneous paravalvular leak (PVL) closure is an alternative treatment option for severely symptomatic, high‐surgical risk patients with PVL. Some patients require multiple percutaneous PVL closure procedures. However, the procedural characteristics and success rate of re‐do PVL closure have not been well studied.AimsThe aim of this study is to investigate the indications, procedural characteristics, technical success rate, and 30‐day major adverse cardiovascular events (MACE) in patients who underwent re‐do PVL closure.MethodsConsecutive patients who underwent percutaneous paramitral or paraaortic leak closure at Mayo Clinic, Rochester, Minnesota from 2004 through 2015 were studied. Clinical and procedural characteristics of patients who underwent re‐do PVL closure were compared to age‐ and sex‐matched patients who underwent their first percutaneous PVL closure. Procedure success and 30‐day MACE were compared in both the groups.ResultsAmong 223 identified percutaneous PVL closures, 16 (7%) were re‐do procedures. Patients who underwent re‐do PVL closure were predominantly men (87.5%) with a mean age of 68 ± 15 years and an estimated mean Society of Thoracic Surgery (STS) 30‐day mortality of 4.6 ± 2.8. Half of the re‐do PVL closures were performed on mechanical valves and 62.5% were performed on paramitral defects. Indications for re‐do PVL closure were: (1) emergence of new significant paravalvular defects in 50%, (2) incomplete index defect closure in 43.75%, and (3) index procedural complication in 6.25% of the cases. Procedural success was 75% in re‐do procedures vs. 85.4% in age‐ and sex‐matched control group (P = 0.45). Thirty‐day MACE was 12.5% in the re‐do group compared to 4.2% in the age‐ and‐sex matched patients who underwent PVL closure for the first time (P = 0.35).ConclusionRe‐do percutaneous PVL closure is feasible with favorable procedural success rate and low 30‐day MACE. Development of new paravalvular defects is the most common indication for re‐do PVL closure, highlighting the importance of careful longitudinal monitoring and follow‐up. © 2017 Wiley Periodicals, Inc.
Extensive Sodium Metal Plating and Stripping in a Highly Concentrated Inorganic−Organic Ionic Liquid Electrolyte through Surface Pretreatment
Authors: Basile Andrew , Makhlooghiazad Faezeh , Yunis Ruhamah , MacFarlane Douglas R. , Forsyth Maria , Howlett Patrick C. 
Published: February 2017

AbstractIn the present work, the stable and extensive cycling of Na plating and stripping is demonstrated within an inorganic−organic ionic liquid electrolyte. The electrolyte is composed of liquid‐state tri(isobutyl)methylphosphonium bis(fluorosulfonyl)imide when employing a high 45 mol % Na concentration in the form of sodium bis(fluorosulfonyl)imide. The liquid‐state electrolyte supports safe Na metal electrochemistry at 50 °C and exhibits a conductivity of 10−2.5 S cm−1. Through plating/stripping with 1 h polarization times (12 cycles, J=0.5 mA cm−2), a surface is prepared with a smooth surface devoid of dendrites and, importantly, a stable solid‐electrolyte interphase, determined by using scanning electron microscopy and impedance spectroscopy. After this, a greater mass transfer can be undertaken through an increased polarization time of 5 h (Q=2.5 mAh cm−2) for a duration of 120 h.
Self‐Assembly of 9,10‐Bis(phenylethynyl) Anthracene (BPEA) Derivatives: Influence of π–π and Hydrogen‐Bonding Interactions on Aggregate Morphology and Self‐Assembly Mechanism
Authors: Lübtow Michael , Helmers Ingo , Stepanenko Vladimir , Albuquerque Rodrigo Q. , Marder Todd B. , Fernández Gustavo 
Published: February 2017

Abstract9,10‐Bis(phenylethynyl)anthracenes (BPEAs) are an important class of dyes in various applications including chemiluminescence emitters, materials for photon upconversion, and for optoelectronic devices. Some of these applications require control over the packing modes of the molecules within the active layer, which can be effected by bottom‐up self‐assembly. Studies aimed at controlling the molecular organization of BPEAs have primarily focused on bulk or liquid‐crystal materials, whereas in‐depth investigations of BPEA‐based assemblies in solution remain elusive. In this article, the self‐assembly of two new BPEA derivatives with hydrophobic side chains is reported, one of them featuring amide functional groups and the other one lacking them. Comparison of the self‐assembly behavior of both systems in solution using spectroscopic (UV/Vis, fluorescence, and NMR), microscopic (AFM), and theoretical (PM6) studies reveals the crucial role of the amide groups in controlling the self‐assembly. For both systems, the formation of H‐type face‐to‐face π‐stacks is proposed, whereas the interplay of π‐stacking and H‐bonding is responsible for driving the formation of 1D stacks and increasing the binding constant by two to three orders of magnitude. Our findings show that H‐bonding is a prerequisite to create ordered BPEA assemblies in solution.
Regioselective Bromination of Benzocycloheptadienones for the Synthesis of Substituted 3,4‐Benzotropolones Including Goupiolone A
Authors: Arican Deniz , Braukmüller Stefan , Brückner Reinhard 
Published: February 2017

AbstractType‐18 or ‐23 benzocycloheptadienones are readily prepared by ring‐closing olefin metatheses. Adding Br2 to 23 and eliminating HBr gave the bromoolefin 28 using DBU or its isomer iso‐28 using DABCO, both with near‐perfect regiocontrol. Both 28 and iso‐28 underwent Sonogashira, Suzuki, Negishi, and Heck couplings as well as Pd‐catalyzed alkoxycarbonylations. Hydrolysis of the resulting α‐ketoketals and enolization of the liberated α‐diketones delivered a portfolio of hitherto unknown 3,4‐benzotropolones. The 8‐ethoxycarbonylated dimethyl‐3,4‐benzotropolone 50 obtained by this route was dimethylated to give goupiolone A (52). This synthesis encompasses 9 steps from 22, that is, half as many as the only previous synthesis (19 steps). A variant of our route afforded the 1,8‐dibromide 54. Coupling with excess phenylboronic acid and ketal hydrolysis provided the diphenylated benzotropolone 56 and suggests a strategy, by which the natural bispulvinone aurantricholone (7) might be reached.
Stream‐based live data replication approach of in‐memory cache
Authors: Ma Kun , Yang Bo 
Published: February 2017

SummaryReplication is a method to keep the consistency of source data and target data. In our previous work of access‐aware in‐memory data cache middleware for relational databases, the data are easy to be lost in case that power cuts off. Therefore, we investigate a live data replication approach from in‐memory data cache to versioning repository in this paper. This method attempts to recover the in‐memory data cache from the versioning repository in failure of access‐aware in‐memory data cache middleware. Although the replication is not a new problem, the state of art of the replication in the context of document stores is not mature. In our paper, we propose a live data replication approach of in‐memory document stores using stream processing framework. First, we introduce cell state model to describe the replication process. To infinitely look back to any revision, we enable our proposed cell state model to support copy‐modify‐merge model to manage the changed data revisions subsequently. Finally, experimental results show that this approach is more suitable for the replication of continuous in‐stream changed data compared with MapReduce‐based batch replication.