bluuebunny/crossref_metadata_2025_split huggingface dataset:
Computational modelling of meiotic entry and commitment
Authors: Bhola Tanvi , Kapuy Orsolya , Vinod P. K. 
Published: January 2018

AbstractIn response to developmental and environmental conditions, cells exit the mitotic cell cycle and enter the meiosis program to generate haploid gametes from diploid germ cells. Once cells decide to enter the meiosis program they become irreversibly committed to the completion of meiosis irrespective of the presence of cue signals. How meiotic entry and commitment occur due to the dynamics of the regulatory network is not well understood. Therefore, we constructed a mathematical model of the regulatory network that controls the transition from mitosis to meiosis in Schizosaccharomyces pombe. Upon nitrogen starvation, yeast cells exit mitosis and undergo conjugation and meiotic entry. The model includes the regulation of Mei2, an RNA binding protein required for conjugation and meiotic entry, by multiple feedback loops involving Pat1, a kinase that keeps cells in mitosis, and Ste11, a transcription activator required for the sexual differentiation. The model accounts for various experimental observations and demonstrates that the activation of Mei2 is bistable, which ensures the irreversible commitment to meiosis. Further, we show by integrating the meiosis-specific regulation with a cell cycle model, the dynamics of cell cycle exit, G1 arrest and entry into meiosis under nitrogen starvation.
Tunable geometrical frustration in magnonic vortex crystals
Authors: Behncke Carolin , Adolff Christian F. , Wintz Sebastian , Hänze Max , Schulte Benedikt , Weigand Markus , Finizio Simone , Raabe Jörg , Meier Guido 
Published: January 2018

AbstractA novel approach to investigate geometrical frustration is introduced using two-dimensional magnonic vortex crystals. The frustration of the crystal can be manipulated and turned on and off dynamically on the timescale of milliseconds. The vortices are studied using scanning transmission x-ray microscopy and ferromagnetic resonance spectroscopy. They are arranged analogous to the nanomagnets in artificial spin-ice systems. The polarization state of the vortices is tuned in a way that geometrical frustration arises. We demonstrate that frustrated polarization states and non-frustrated states can be tuned to the crystal by changing the frequency of the state formation process.
Single, very low rituximab doses in healthy volunteers - a pilot and a randomized trial: implications for dosing and biosimilarity testing
Authors: Schoergenhofer Christian , Schwameis Michael , Firbas Christa , Bartko Johann , Derhaschnig Ulla , Mader Robert M , Plaßmann Raute Sunder , Jilma-Stohlawetz Petra , Desai Kalpna , Misra Priya , Jäger Ulrich , Jilma Bernd 
Published: January 2018

AbstractThere are no dose-finding trials available for rituximab that could guide dosing in non-malignant diseases. We hypothesized that currently used doses (≥375 mg/m2) exceed several hundred-fold the half-maximal effective dose, which is most sensitive for detecting putative differences between biosimilars and important for dose finding. In an open label, exploratory trial healthy volunteers received single infusions of rituximab at doses of 0.1, 0.3 or 1.0 mg/m2. Subsequently, in a double-blind, randomized trial healthy volunteers received single infusions of two rituximab products at doses of 0.1 and 0.3 mg/m2. In the exploratory trial rituximab transiently depleted CD20+ cells by a mean 68% (range: 57–95%), 74% (55–82%) and 97% (94–100%) immediately after the infusion of 0.1 (n = 4), 0.3 (n = 4) and 1 mg/m2 (n = 8), respectively. In the randomized trial CD20+ cells decreased by a mean 48% (25–84%) − 55% (26–85%) and 81 (67–89%) – 87% (77–96%) after infusion of 0.1 mg/m2 (n = 12) or 0.3 mg/m2 (n = 8 proposed biosimilar, n = 4 reference product) of the proposed biosimilar or the reference product, respectively. It is important to understand that in healthy volunteers <1% of the authorized rituximab doses depletes almost all circulating B lymphocytes. Thus, for non-malignant diseases alternative, more cost-effective dosing regimens seem plausible, but require clinical testing. (EudraCT-No. 2010–023781–45; EudraCT-No. 2013–001077–24).
Precise phase demodulation of single carrier-frequency interferogram by pixel-level Lissajous figure and ellipse fitting
Authors: Liu Fengwei , Wu Yongqian , Wu Fan , König Niels , Schmitt Robert , Wan Yongjian , Xu Yan 
Published: January 2018

AbstractPhase demodulation from a single carrier-frequency fringe pattern is becoming increasingly important particularly in areas of optical metrology such as dynamic interferometry, deflectometry and profilometry. The Fourier transform (FT) method and the spatial-carrier phase-shifting technique (SCPS) are two popular and well-established approaches to demodulation. However FT has the drawback of significant edge errors because of the Gibbs effect, whilst detuning errors for the local phase shift occur when SCPS is applied. A novel demodulation method based on pixel-level Lissajous figure and ellipse fitting (PLEF) is presented in this paper. Local demodulation in the spatial domain makes PLEF more flexible than the FT method, without spectral leakage. Based on a more adaptable approach, account is taken of variations in illumination and phase distribution over a few neighboring pixels. The mathematic demodulation model is of interest and has been demonstrated via simulation. Theoretical phase extraction error is as low as 10−4 rad. Experiments further corroborate the effectiveness of the proposed method. In conclusion, various influencing factors, e.g. variations of background/modulation, phase amplitude, carrier frequency, additive noise that may affect the precision of PLEF are discussed in detail.
Polyaniline cryogels: Biocompatibility of novel conducting macroporous material
Authors: Humpolíček Petr , Radaszkiewicz Katarzyna Anna , Capáková Zdenka , Pacherník Jiří , Bober Patrycja , Kašpárková Věra , Rejmontová Petra , Lehocký Marián , Ponížil Petr , Stejskal Jaroslav 
Published: January 2018

AbstractPolyaniline cryogel is a new unique form of polyaniline combining intrinsic electrical conductivity and the material properties of hydrogels. It is prepared by the polymerization of aniline in frozen poly(vinyl alcohol) solutions. The biocompatibility of macroporous polyaniline cryogel was demonstrated by testing its cytotoxicity on mouse embryonic fibroblasts and via the test of embryotoxicity based on the formation of beating foci within spontaneous differentiating embryonic stem cells. Good biocompatibility was related to low contents of low-molecular-weight impurities in polyaniline cryogel, which was confirmed by liquid chromatography. The adhesion and growth of embryonic stem cells, embryoid bodies, cardiomyocytes, and neural progenitors prove that polyaniline cryogel has the potential to be used as a carrier for cells in tissue engineering or bio-sensing. The surface energy as well as the elasticity and porosity of cryogel mimic tissue properties. Polyaniline cryogel can therefore be applied in bio-sensing or regenerative medicine in general, and mainly in the tissue engineering of electrically excitable tissues.
The epidemiology of hepatitis C virus in Iran: Systematic review and meta-analyses
Authors: Mahmud Sarwat , Akbarzadeh Vajiheh , Abu-Raddad Laith J. 
Published: January 2018

AbstractThe aim of this study was to characterize hepatitis C virus (HCV) epidemiology in Iran and estimate the pooled mean HCV antibody prevalence in different risk populations. We systematically reviewed and synthesized reports of HCV incidence and/or prevalence, as informed by the Cochrane Collaboration Handbook, and reported our findings following the PRISMA guidelines. DerSimonian-Laird random effects meta-analyses were implemented to estimate HCV prevalence in various risk populations. We identified five HCV incidence and 472 HCV prevalence measures. Our meta-analyses estimated HCV prevalence at 0.3% among the general population, 6.2% among intermediate risk populations, 32.1% among high risk populations, and 4.6% among special clinical populations. Our meta-analyses for subpopulations estimated HCV prevalence at 52.2% among people who inject drugs (PWID), 20.0% among populations at high risk of healthcare-related exposures, and 7.5% among populations with liver-related conditions. Genotype 1 was the most frequent circulating strain at 58.2%, followed by genotype 3 at 39.0%. HCV prevalence in the general population was lower than that found in other Middle East and North Africa countries and globally. However, HCV prevalence was high in PWID and populations at high risk of healthcare-related exposures. Ongoing transmission appears to be driven by drug injection and specific healthcare procedures.
Effect of microstructure on the impact toughness and temper embrittlement of SA508Gr.4N steel for advanced pressure vessel materials
Authors: Yang Zhiqiang , Liu Zhengdong , He Xikou , Qiao Shibin , Xie Changsheng 
Published: January 2018

AbstractThe effect of microstructure on the impact toughness and the temper embrittlement of a SA508Gr.4N steel was investigated. Martensitic and bainitic structures formed in this material were examined via scanning electron microscopy, electron backscatter diffraction, transmission electron microscopy, and Auger electron spectroscopy (AES) analysis. The martensitic structure had a positive effect on both the strength and toughness. Compared with the bainitic structure, this structure consisted of smaller blocks and more high-angle grain boundaries (HAGBs). Changes in the ultimate tensile strength and toughness of the martensitic structure were attributed to an increase in the crack propagation path. This increase resulted from an increased number of HAGBs and refinement of the sub-structure (block). The AES results revealed that sulfur segregation is higher in the martensitic structure than in the bainitic structure. Therefore, the martensitic structure is more susceptible to temper embrittlement than the bainitic structure.
Connexin 43 Hemichannel as a Novel Mediator of Sterile and Infectious Inflammatory Diseases
Authors: Li Wei , Bao Guoqiang , Chen Weiqiang , Qiang Xiaoling , Zhu Shu , Wang Shuaiwei , He Mingzhu , Ma Gaifeng , Ochani Mahendar , Al-Abed Yousef , Yang Huan , Tracey Kevin J. , Wang Ping , D’Angelo John , Wang Haichao 
Published: January 2018

AbstractCytoplasmic membrane-bound connexin 43 (Cx43) proteins oligomerize into hexameric channels (hemichannels) that can sometimes dock with hemichannels on adjacent cells to form gap junctional (GJ) channels. However, the possible role of Cx43 hemichannels in sterile and infectious inflammatory diseases has not been adequately defined due to the lack of selective interventions. Here we report that a proinflammatory mediator, the serum amyloid A (SAA), resembled bacterial endotoxin by stimulating macrophages to up-regulate Cx43 expression and double-stranded RNA-activated protein kinase R (PKR) phosphorylation in a TLR4-dependent fashion. Two well-known Cx43 mimetic peptides, the GAP26 and TAT-GAP19, divergently affected macrophage hemichannel activities in vitro, and differentially altered the outcome of lethal sepsis in vivo. By screening a panel of Cx43 mimetic peptides, we discovered that one cysteine-containing peptide, P5 (ENVCYD), effectively attenuated hemichannel activities, and significantly suppressed endotoxin-induced release of ATP and HMGB1 in vitro. In vivo, the P5 peptide conferred a significant protection against hepatic ischemia/reperfusion injury and lethal microbial infection. Collectively, these findings have suggested a pathogenic role of Cx43 hemichannels in sterile injurious as well as infectious inflammatory diseases possibly through facilitating extracellular ATP efflux to trigger PKR phosphorylation/activation.
Active Terahertz Chiral Metamaterials Based on Phase Transition of Vanadium Dioxide (VO2)
Authors: Wang Shengxiang , Kang Lei , Werner Douglas H. 
Published: January 2018

AbstractCompared with natural materials, chiral metamaterials have been demonstrated with orders of magnitude stronger chiroptical response, which provides the basis for applications such as ultracompact polarization components and plasmonic-enhanced biosensing. Terahertz chiral metamaterials that allow dynamic polarization control of terahertz waves are of great practical interest, but remain extremely rare. Here, we show that hybrid metamaterials integrated with vanadium dioxide (VO2) exhibiting phase transition can enable dynamically tunable chiroptical responses at terahertz frequencies. In particular, a circular dichroism of ~40° and a maximum polarization rotation of ~200°/λ are observed around 0.7 THz. Furthermore, our study also reveals that the chiroptical response from the proposed metamaterials is strongly dependent on the phase transition of VO2, leading to actively controllable polarization states of the transmitted terahertz waves. This work paves the way for the development of terahertz metadevices capable of enabling active polarization manipulation.
Revisiting the link between body and agency: visual movement congruency enhances intentional binding but is not body-specific
Authors: Zopf Regine , Polito Vince , Moore James 
Published: January 2018

AbstractEmbodiment and agency are key aspects of how we perceive ourselves that have typically been associated with independent mechanisms. Recent work, however, has suggested that these mechanisms are related. The sense of agency arises from recognising a causal influence on the external world. This influence is typically realised through bodily movements and thus the perception of the bodily self could also be crucial for agency. We investigated whether a key index of agency – intentional binding – was modulated by body-specific information. Participants judged the interval between pressing a button and a subsequent tone. We used virtual reality to manipulate two aspects of movement feedback. First, form: participants viewed a virtual hand or sphere. Second, movement congruency: the viewed object moved congruently or incongruently with the participant’s hidden hand. Both factors, form and movement congruency, significantly influenced embodiment. However, only movement congruency influenced intentional binding. Binding was increased for congruent compared to incongruent movement feedback irrespective of form. This shows that the comparison between viewed and performed movements provides an important cue for agency, whereas body-specific visual form does not. We suggest that embodiment and agency mechanisms both depend on comparisons across sensorimotor signals but that they are influenced by distinct factors.
RETRACTED ARTICLE: Chemokine receptor 7 overexpression promotes mesenchymal stem cell migration and proliferation via secreting Chemokine ligand 12
Authors: Liu Ling , Chen Jian-Xiao , Zhang Xi-Wen , Sun Qin , Yang Lan , Liu Airan , Hu Shuling , Guo Fengmei , Liu Songqiao , Huang Yingzi , Yang Yi , Qiu Hai-Bo 
Published: January 2018

AbstractGreat interest has been shown in mesenchymal stem cell (MSC) therapy in a wide variety of clinical domains. However, the therapeutic efficiency depends on the proliferation and migration of MSCs. Chemokine receptors are involved in regulating the proliferation and migration to the specific organs of MSCs in different microenvironments. CXC receptor seven (CXCR7), a newly discovered Chemokine ligand 12 (CXCL12) receptor, has organ specificity for tumour migration. We hypothesized that CXCR7 expression affects proliferation and migration of MSCs. In present study, we constructed long-term and stable mMSCs lines overexpressing and suppressing CXCR7 modifications with lentiviral vectors. The transduction efficiencies, mRNA and protein expression of CXCR7 were significantly regulated. CXCR7 gene overexpression promoted mMSCs proliferation and migration, whereas suppressing CXCR7 had the opposite effect. Additional CXCL12 improved the vertical migration of mMSCs. The overexpression of CXCR7 increased the MSC-secreted CXCL12, VCAM-1, CD44 and MMP2 levels, which contributed to the improvement of mMSC proliferation and migration. Therefore, overexpressing CXCR7 improved the proliferation and migration of mMSCs, which may be attributable to the CXCL12 secreted by MSCs, leading to a positive feedback loop for CXCL12/CXCR7 axis. Our results may provide a potential method for improving the treatment effectiveness of mMSCs by overexpressing CXCR7.
Physiological response and microRNA expression profiles in head kidney of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to acute cold stress
Authors: Qiang Jun , Cui Yan T. , Tao Fan Y. , Bao Wen J. , He Jie , Li Xia H. , Xu Pao , Sun Lan Y. 
Published: January 2018

AbstractCold stress has a serious impact on the overwintering survival and yield of genetically improved farmed tilapia (GIFT,Oreochromis niloticus). Understanding the physiological and molecular regulation mechanisms of low-temperature adaptation is necessary to help breed new tolerant strains. The semi-lethal low temperature of juvenile GIFT at 96 h was determined as 9.4 °C. We constructed and sequenced two small RNA libraries from head kidney tissues, one for the control (CO) group and one for the 9.4 °C-stressed (LTS) group, and identified 1736 and 1481 known microRNAs (miRNAs), and 164 and 152 novel miRNAs in the CO and LTS libraries, respectively. We verify the expression of nine up-regulated miRNAs and eight down-regulation miRNAs by qRT-PCR, and found their expression patterns were consistent with the sequencing results. We found that cold stress may have produced dysregulation of free radical and lipid metabolism, decreased superoxide dismutase activity, reduced respiratory burst and phagocytic activity of macrophages, increased malondialdehyde content, and adversely affected the physiological adaptation of GIFT, eventually leading to death. This study revealed interactions among miRNAs and signal regulated pathways in GIFT under cold stress that may help to understand the pathways involved in cold resistance.
A Mosquito Inspired Strategy to Implant Microprobes into the Brain
Authors: Shoffstall Andrew J. , Srinivasan Suraj , Willis Mitchell , Stiller Allison M. , Ecker Melanie , Voit Walter E. , Pancrazio Joseph J. , Capadona Jeffrey R. 
Published: January 2018

AbstractMosquitos are among the deadliest insects on the planet due to their ability to transmit diseases like malaria through their bite. In order to bite, a mosquito must insert a set of micro-sized needles through the skin to reach vascular structures. The mosquito uses a combination of mechanisms including an insertion guide to enable it to bite and feed off of larger animals. Here, we report on a biomimetic strategy inspired by the mosquito insertion guide to enable the implantation of intracortical microelectrodes into the brain. Next generation microelectrode designs leveraging ultra-small dimensions and/or flexible materials offer the promise of increased performance, but present difficulties in reliable implantation. With the biomimetic guide in place, the rate of successful microprobe insertion increased from 37.5% to 100% due to the rise in the critical buckling force of the microprobes by 3.8-fold. The prototype guides presented here provide a reproducible method to augment the insertion of small, flexible devices into the brain. In the future, similar approaches may be considered and applied to the insertion of other difficult to implant medical devices.
Loss of the Mitochondrial Fatty Acid β-Oxidation Protein Medium-Chain Acyl-Coenzyme A Dehydrogenase Disrupts Oxidative Phosphorylation Protein Complex Stability and Function
Authors: Lim Sze Chern , Tajika Makiko , Shimura Masaru , Carey Kirstyn T. , Stroud David A. , Murayama Kei , Ohtake Akira , McKenzie Matthew 
Published: January 2018

AbstractMedium-chain acyl-Coenzyme A dehydrogenase (MCAD) is involved in the initial step of mitochondrial fatty acid β-oxidation (FAO). Loss of function results in MCAD deficiency, a disorder that usually presents in childhood with hypoketotic hypoglycemia, vomiting and lethargy. While the disruption of mitochondrial fatty acid metabolism is the primary metabolic defect, secondary defects in mitochondrial oxidative phosphorylation (OXPHOS) may also contribute to disease pathogenesis. Therefore, we examined OXPHOS activity and stability in MCAD-deficient patient fibroblasts that have no detectable MCAD protein. We found a deficit in mitochondrial oxygen consumption, with reduced steady-state levels of OXPHOS complexes I, III and IV, as well as the OXPHOS supercomplex. To examine the mechanisms involved, we generated an MCAD knockout (KO) using human 143B osteosarcoma cells. These cells also exhibited defects in OXPHOS complex function and steady-state levels, as well as disrupted biogenesis of newly-translated OXPHOS subunits. Overall, our findings suggest that the loss of MCAD is associated with a reduction in steady-state OXPHOS complex levels, resulting in secondary defects in OXPHOS function which may contribute to the pathology of MCAD deficiency.
Power and chirp effects on the frequency stability of resonant dispersive waves generated in photonic crystal fibres
Authors: Cao Tao , Liu Mingchen , Xu Chang , Yan Jikun , Shen Chaochao , Liu Shaozhen , Peng Hao , Peng Jiahui , Sokolov Alexei V. 
Published: January 2018

AbstractOptimization of laser output parameters vs. f-to-2f beating signals can be mutually contradicting, when an octave-spanning supercontinuum is employed for f-to-2f measurements. We show that resonant dispersive waves will solve this issue, thanks to their frequency stability against changes in laser power and chirping.
Application of Rotating Magnetic Fields Increase the Activity of Antimicrobials Against Wound Biofilm Pathogens
Authors: Junka A. F. , Rakoczy R. , Szymczyk P. , Bartoszewicz M. , Sedghizadeh P. P. , Fijałkowski K. 
Published: January 2018

AbstractInfective complications are a major factor contributing to wound chronicity and can be associated with significant morbidity or mortality. Wound bacteria are protected in biofilm communities and are highly resistant to immune system components and to antimicrobials used in wound therapy. There is an urgent medical need to more effectively eradicate wound biofilm pathogens. In the present work, we tested the impact of such commonly used antibiotics and antiseptics as gentamycin, ciprofloxacin, octenidine, chlorhexidine, polihexanidine, and ethacridine lactate delivered to Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the presence of rotating magnetic fields (RMFs) of 10–50 Hz frequency and produced by a customized RMF generator. Fifty percent greater reduction in biofilm growth and biomass was observed after exposure to RMF as compared to biofilms not exposed to RMF. Our results suggest that RMF as an adjunct to antiseptic wound care can significantly improve antibiofilm activity, which has important translational potential for clinical applications.
Influence of In-Gap States on the Formation of Two-Dimensional Election Gas at ABO3/SrTiO3 Interfaces
Authors: Li Cheng-Jian , Xue Hong-Xia , Qu Guo-Liang , Shen Sheng-Chun , Hong Yan-Peng , Wang Xin-Xin , Liu Ming-rui , Jiang Wei-min , Badica Petre , He Lin , Dou Rui-Fen , Xiong Chang-Min , Lü Wei-ming , Nie Jia-Cai 
Published: January 2018

AbstractWe explored in-gap states (IGSs) in perovskite oxide heterojunction films. We report that IGSs in these films play a crucial role in determining the formation and properties of interfacial two-dimensional electron gas (2DEG). We report that electron trapping by IGSs opposes charge transfer from the film to the interface. The IGS in films yielded insulating interfaces with polar discontinuity and explained low interface carrier density of conducting interfaces. An ion trapping model was proposed to explain the physics of the IGSs and some experimental findings, such as the unexpected formation of 2DEG at the initially insulating LaCrO3/SrTiO3 interface and the influence of substitution layers on 2DEG.