bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Evaluation of Fibrin-Related Markers for Diagnosing or Predicting Acute or Subclinical Venous Thromboembolism in Patients Undergoing Major Orthopedic Surgery
Authors: Hasegawa Masahiro , Wada Hideo , Miyazaki Shinichi , Yamaguchi Toshio , Wakabayashi Hiroki , Fujimoto Naoki , Matsumoto Takeshi , Ohishi Kohshi , Sakaguchi Akane , Yamada Norikazu , Ito Masaaki , Yamashita Yoshiki , Katayama Naoyuki , Nakatani Kaname , Sudo Akihiro 
Published: October 2016

Objective: The cutoff values of fibrin-related markers (FRMs) diagnosing or predicting the occurrence of a venous thromboembolism (VTE) were evaluated. Materials and Methods: Fibrin-related markers such as fibrin monomer complex (FMC), D-dimer, and fibrinogen and fibrin degradation products (FDPs) before surgery were measured in 326 patients undergoing orthopedic surgery to diagnose subclinical VTE or predict postoperative VTE. Results: Although the FMC, D-dimer, and FDP levels were all useful for the diagnosis of acute VTE, the FDP level was not useful for diagnosing subclinical VTE or predicting postoperative VTE. The results of several D-dimer assays closely correlated with other D-dimer assays. There were various cutoff ranges for diagnosing or predicting VTE. Some D-dimer assays were useful for diagnosing low levels of D-dimer and others were useful for diagnosing moderate to high D-dimer levels. Increased D-dimer levels were useful for diagnosing acute (cutoff values: 2.0-5.9 μg/mL) or about 10% of subclinical VTE (cutoff values: 3.4-5.3 μg/mL), for predicting about 10% of postoperative VTE (cutoff values: 3.4-5.3 μg/mL), and for excluding VTE. Conclusion: Although increased D-dimer levels were useful for diagnosing subclinical VTE and predicting the risk of VTE, there were various cutoff values for the diagnosis or prediction of VTE.
Lobbying Across Venues: An Issue-Tracing Approach
Authors: Jourdain Charlotte , Hug Simon , Varone Frédéric 
Published: October 2016

AbstractThis study examines lobbying activity during four California policymaking processes and through the four institutional venues available in that state: the legislative, executive, and judicial branches, and the ballot initiative. It shows that past advocacy activity explains future mobilization on the same policy issue. Groups that fail to reach their policy goals will be more likely to mobilize later if the policy process changes venue, compared with those that have achieved their policy preference. Thus, the availability of multiple venues provides a counterweight to the possible advantages received by certain group types in each venue. Furthermore, public interest groups are more likely to mobilize across venues and repeatedly within a venue, while business groups are less likely to do so.
Heat delays skin wound healing in mice
Authors: dos Santos-Silva Marco Aurélio , Trajano Eduardo Tavares Lima , Schanuel Fernanda Seabra , Monte-Alto-Costa Andréa 
Published: October 2016

In vivo studies have shown that the combination of infrared radiation (IR) and visible light (VIS) is responsible for the activation of metaloproteinases, causing matrix degradation and damage to healthy skin. However, the role of heat originating from the VIS spectrum on wound healing remains poorly understood. Our objective was to investigate the macroscopic, microscopic and biochemical effects of heat induced by visible light on cutaneous wound healing in mice. Male mice were anesthetized, subjected to a cutaneous excisional wound and divided into two groups ( n = 10/group) exposed to 23℃ or 43℃ in a thermal chamber for 30 min every other day, for 13 days. On day 14, the animals were sacrificed, and their lesions were processed for histochemistry, immunohistochemistry and protein expression analysis. The wound area was 42% greater 11 days ( p < 0.01) and 29% greater 14 days ( p < 0.001) after wounding in the 43℃ group than in the 23℃ group. The 43℃ group presented a lower (17%) percentage of reepithelialized wounds ( p < 0.001) 14 days after wounding. The length of the epidermal gap was greater in the 43℃ group ( p < 0.01). The volume density of myofibroblasts and the number of F4/80-positive macrophages was greater in the 43℃ group ( p < 0.05). The 43℃ group showed increased protein expression of type III collagen ( p < 0.001), decreased protein expression of type I collagen ( p < 0.05), increased MMP-1 expression ( p < 0.05), and decreased MMP-2 activity ( p < 0.001). The protein expression of fibrillin-1 ( p < 0.001), MMP-12 ( p < 0.05), TGF-β 1/2/3 ( p < 0.01) and ERK activation ( p < 0.05) was increased in the 43℃ group. Our results suggest that heat delays the stages of wound healing in mice.
Exposure to secondhand smoke and risk of peripheral arterial disease in southern Chinese non-smokers: The Guangzhou Biobank Cohort Study-Cardiovascular Disease Sub-cohort
Authors: Lu Liya , Jiang Chaoqiang , Mackay Danny F , Pell Jill P , Cheng Kar Keung , Lam Tai Hing , Thomas G Neil 
Published: October 2016

Objectives We studied the association between secondhand smoke (SHS) exposure and peripheral arterial disease (PAD) in Chinese non-smokers. Methods We conducted a cross-sectional study using baseline data from the Guangzhou Biobank Cohort Study: Cardiovascular Disease Sub-cohort Study (GBCS-CVD). Guangzhou residents aged ≥ 50 years were recruited between 2003 and 2008. Baseline data included measurement of ankle brachial pressure index (ABPI) and self-reported smoking status and SHS exposure. Univariate and multivariate logistic regression analyses were used to analyze the association between SHS and PAD (defined as ABPI < 0.9). Results Of the 1507 non-smokers, 24 (1.6%) had PAD. Of these, 12 were men and 12 were women. Exposure to SHS at home of ≥25 h per week was reported by 16.7% of PAD cases compared with 3.8% of those without PAD (χ2 test, p = 0.003). After adjustment for potential confounders, exposure to ≥25 h per week at home was still associated with PAD (adjusted OR 7.86, 95% CI 2.00–30.95, p = 0.003), with suggestion of a dose-response relationship. Conclusions Our results extend the US Surgeon General’s 2006 report that SHS exposure is an independent risk factor for PAD. National smoke-free legislation is needed to protect all people from exposure.
The correlation of the platelet-to-lymphocyte ratio with the severity of stenosis and stroke in patients with carotid arterial disease
Authors: İdil Soylu Ayşegül , Arıkan Cortcu Sümeyra , Uzunkaya Fatih , Atalay Yunus Oktay , Bekçi Tumay , Güngör Levent , Belet Ümit 
Published: October 2016

Objectives Platelet-to-lymphocyte ratio is a novel biomarker, recently shown to be correlated with atherosclerotic inflammation. This study investigated the role of platelet-to-lymphocyte ratio in patients with carotid artery stenosis and stroke. Methods Patients, who underwent carotid angiography with Multiple Detector Computed Tomography Angiography at our hospital, were retrospectively screened. Patients enrolled were divided into three groups based on the platelet-to-lymphocyte ratio. Patients with a platelet-to-lymphocyte ratio value between 55.0 and 106.71 were assigned to Group I, patients with a platelet-to-lymphocyte ratio value between 106.79 and 160.61 were assigned to Group II and patients with a platelet-to-lymphocyte ratio value between 162.96 and 619.61 were assigned to Group III. The carotid arterial stenosis calculated was classified as per the criteria of North American Symptomatic Carotid Endarterectomy Trial. Results One hundred fifty patients were included in our trial (mean age 61.9 ± 13.1 with 104 males). The rate of carotid arterial stenosis was detected to be higher in patients with a high platelet-to-lymphocyte ratio value (p = 0.010). Additionally, the platelet-to-lymphocyte ratio was positively correlated with the carotid arterial stenosis percentage (r = 0.250, p = 0.002). In the multi-variate regression analysis, platelet-to-lymphocyte ratio was detected to be an independent variable with respect to stroke (odd’s ratio = 1.012, confidence interval = 1.001–1.024, p = 0.031). Conclusions Increased platelet-to-lymphocyte ratio could be a simple and practical marker of the clinical course in patients with carotid arterial stenosis.
Management of anaemia in oncohaematological patients treated with biosimilar epoetin alfa: results of an Italian observational, retrospective study
Authors: Rosti Giovanni , Petrini Mario , Bosi Alberto , Galieni Piero , Bernardi Daniele , Giglio Gianfranco , Dorotea Laura , Falini Brunangelo , Scelzi Elvira , Veltri Enzo , Castelli Roberto , Longagnani Chiara , Raggi Tommaso , Simonetti Federico 
Published: October 2016

Background: Many patients with solid tumours or nonmyeloid haematopoietic tumours develop symptomatic anaemia, which has a major impact on quality of life (QoL). The efficacy of erythropoiesis-stimulating agents (ESAs) in improving QoL and reducing blood transfusions has been widely demonstrated. Binocrit® (biosimilar epoetin alfa) is an ESA indicated in the European Union for treating chemotherapy-induced anaemia. The aim of this study was to investigate the effect of Binocrit® on haemoglobin (Hb) levels in anaemic cancer patients in Italian clinical practice. Methods: The ANEMONE study was a national, longitudinal, retrospective, multicentre observational study. Patients had to be 18 years or older, with a solid tumour or non-Hodgkin’s lymphoma, Hodgkin’s disease or multiple myeloma, receiving chemotherapy, and treated with Binocrit® to manage chemotherapy-induced anaemia. The primary outcomes were the proportion of patients with a Hb increase ⩾1 g/dl during the first 4 weeks and with a Hb increase ⩾2 g/dl during the first 12 weeks. Results: A total of 245 patients were enrolled and 215 patients were evaluable for statistical analysis. In the first 4 weeks, 49.3% of patients showed an increase in Hb of ⩾1 g/dl: 45.5% in patients with solid tumours and 52.1% in patients with haematological malignancies. In the first 12 weeks, 51.6% of patients showed an increase in Hb of ⩾2 g/dl (48.4% solid tumours, 54.2% haematological diseases). Treatment with Binocrit® was well tolerated. Conclusions: These results confirm the effectiveness and safety of Binocrit® for chemotherapy-induced anaemia in routine practice in patients with solid tumours, lymphoma and myeloma.
Association Between Meniscal and Chondral Lesions and Timing of Anterior Cruciate Ligament Reconstruction
Authors: de Campos Gustavo Constantino , Nery Wilton , Teixeira Paulo Eduardo Portes , Araujo Paulo Henrique , Alves Wilson de Mello 
Published: October 2016

Background: Rupture of the anterior cruciate ligament (ACL) is a common sports injury and is known to be associated with an increased risk of knee osteoarthritis. Several studies have indicated that the risk of additional injuries to the menisci and articular cartilage increases with delays in the treatment of ACL tears. However, no consensus has been reached regarding the ideal timing for ACL reconstruction in terms of preventing secondary lesions. Purpose: To determine how the time elapsed between an ACL lesion and its reconstruction affects the incidence of meniscal and chondral lesions. Study Design: Case series; Level of evidence, 4. Methods: Medical records of 764 patients who underwent primary ACL reconstruction were reviewed. Data from arthroscopic findings that included information about meniscal lesions and full-thickness articular cartilage lesions at the time of surgery were collected. The association between time elapsed between ACL lesion and reconstruction surgery and incidence of articular cartilage and meniscal lesions was analyzed by chi-square or Fisher exact test. The risk of secondary lesion was calculated by odds ratios (ORs) obtained from simple logistic regression analysis. Results: A positive correlation was observed between time after injury and the presence of any articular lesions ( P = .003), cartilage lesions ( P = .01), and medial meniscus lesions ( P < .001). When analyzing the risk of secondary lesion relative to the reference period (<2 months), it was observed that the odds of finding any articular injury at the time of ACL reconstruction increased when the time from ACL injury to surgery was between 12 and 24 months (OR = 2.62) and >24 months (OR = 5.88). Furthermore, the odds of lesions on the medial meniscus increased when the timing between injury and surgery was 6 to 12 months (OR = 2.71) and continued to increase when the timing was 12 to 24 months (OR = 3.78) and >24 months (OR = 9.07). Conclusion: Associated articular lesions are more common if ACL reconstruction is delayed by ≥6 months (medial meniscus lesion) and ≥1 year (chondral or any meniscal lesion).
Atorvastatin enhances endothelial cell function in posttransplant poor graft function
Authors: Shi Min-Min , Kong Yuan , Song Yang , Sun Yu-Qian , Wang Yu , Zhang Xiao-Hui , Xu Lan-Ping , Liu Kai-Yan , Huang Xiao-Jun 
Published: October 2016

Key Points
Dysfunctional BM EPCs were found in subjects with PGF postallotransplant. BM EPCs from subjects with PGF were enhanced by atorvastatin through downregulation of the p38 MAPK pathway.
Subcellular protein expression models for microsatellite instability in colorectal adenocarcinoma tissue images
Authors: Kovacheva Violeta N. , Rajpoot Nasir M. 
Published: October 2016

Abstract
Background
New bioimaging techniques capable of visualising the co-location of numerous proteins within individual cells have been proposed to study tumour heterogeneity of neighbouring cells within the same tissue specimen. These techniques have highlighted the need to better understand the interplay between proteins in terms of their colocalisation.

Results
We recently proposed a cellular-level model of the healthy and cancerous colonic crypt microenvironments. Here, we extend the model to include detailed models of protein expression to generate synthetic multiplex fluorescence data. As a first step, we present models for various cell organelles learned from real immunofluorescence data from the Human Protein Atlas. Comparison between the distribution of various features obtained from the real and synthetic organelles has shown very good agreement. This has included both features that have been used as part of the model input and ones that have not been explicitly considered. We then develop models for six proteins which are important colorectal cancer biomarkers and are associated with microsatellite instability, namely MLH1, PMS2, MSH2, MSH6, P53 and PTEN. The protein models include their complex expression patterns and which cell phenotypes express them. The models have been validated by comparing distributions of real and synthesised parameters and by application of frameworks for analysing multiplex immunofluorescence image data.

Conclusions
The six proteins have been chosen as a case study to illustrate how the model can be used to generate synthetic multiplex immunofluorescence data. Further proteins could be included within the model in a similar manner to enable the study of a larger set of proteins of interest and their interactions. To the best of our knowledge, this is the first model for expression of multiple proteins in anatomically intact tissue, rather than within cells in culture.
Substance P–mediated chemokine production promotes monocyte migration
Authors: Spitsin Sergei , Meshki John , Winters Angela , Tuluc Florin , Benton Tami D , Douglas Steven D 
Published: October 2016

Abstract
The neuropeptide SP has physiologic and pathophysiologic roles in CNS and peripheral tissues and is involved in crosstalk between nervous and immune systems in various conditions, including HIV and SIV infection. Increased SP levels were demonstrated in plasma of HIV+ individuals as well as in the CNS of SIV-infected, nonhuman primates. SP increases HIV infection in macrophages through interaction with its receptor, NK1R. The SP effect on immune system is both pro- and anti-inflammatory and includes up-regulation of a number of cytokines and cell receptors. The main goal of this study was to determine whether there is interplay between monocyte exposure to SP and recruitment into sites of inflammation. We now demonstrate that exposure of either human macrophages or PBMCs to SP leads to increased production of chemokines, including MCP-1, for which expression is limited to cells of the myeloid lineage. This effect is inhibited by the NK1R antagonist, aprepitant. Exposure to conditioned medium derived from SP-treated PBMCs resulted in increased monocyte migration through semipermeable membranes and an in vitro human BBB model. Monocyte migration was blocked by anti–MCP-1 antibodies. Our results suggest that increased SP levels associated with HIV and other inflammatory conditions may contribute to increased monocyte migration into the CNS and other tissues through a MCP-1–dependent mechanism.
Danger-associated molecular patterns in Alzheimer’s disease
Authors: Venegas Carmen , Heneka Michael T 
Published: October 2016

AbstractPathogen-associated molecular patterns (PAMPs) and endogenous “danger” signals, known as danger-associated molecular patterns (DAMPs), released from cells alert the innate immune system and activate several signal transduction pathways through interactions with the highly conserved pattern recognition receptors (PRRs). Both PAMPs and DAMPs directly induce proinflammatory cascades and trigger the formation of the inflammasome, mediating the release of cytokines. Here, we highlight the role of DAMPs, including amyloid β (Aβ), high-mobility group box 1 (HMGB1), the S100 family proteins, chromogranin A, and nucleic acids, in the innate-immune activation during the course of Alzheimer disease (AD), the most frequent neurodegenerative disorder.
Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes
Authors: Munro Michelle L. , Jayasinghe Izzy , Wang Qiongling , Quick Ann , Wang Wei , Baddeley David , Wehrens Xander H. T. , Soeller Christian 
Published: October 2016

ABSTRACT
Signalling nanodomains requiring close contact between the plasma membrane and internal compartments, known as ‘junctions’, are fast communication hubs within excitable cells such as neurones and muscle. Here, we have examined two transgenic murine models probing the role of junctophilin-2, a membrane-tethering protein crucial for the formation and molecular organisation of sub-microscopic junctions in ventricular muscle cells of the heart. Quantitative single-molecule localisation microscopy showed that junctions in animals producing above-normal levels of junctophilin-2 were enlarged, allowing the re-organisation of the primary functional protein within it, the ryanodine receptor (RyR; in this paper, we use RyR to refer to the myocardial isoform RyR2). Although this change was associated with much enlarged RyR clusters that, due to their size, should be more excitable, functionally it caused a mild inhibition in the Ca2+ signalling output of the junctions (Ca2+ sparks). Analysis of the single-molecule densities of both RyR and junctophilin-2 revealed an ∼3-fold increase in the junctophilin-2 to RyR ratio. This molecular rearrangement is compatible with direct inhibition of RyR opening by junctophilin-2 to intrinsically stabilise the Ca2+ signalling properties of the junction and thus the contractile function of the cell.
Συγκριτική προσέγγιση της επιθετικής συμπεριφοράς των παιδιών με αυτισμό στην σχολική ηλικία
Authors: Νόβα Χρυσή 
Published: October 2016

<p align="center"><strong>Περίληψη</strong></p><p>Η διαταραχή του αυτισμού και το συνδεόμενο με αυτή φαινόμενο της επιθετικότητας αποτελούν τον βασικό πυλώνα της εν λόγω ανακοίνωσης. Ειδικότερα, σύμφωνα με το DSM-IV-TR o αυτισμός ανήκει στην ομάδα των Διάχυτων Αναπτυξιακών Διαταραχών, οι οποίες χαρακτηρίζονται απο διάφορα ελλείματα στην γνωστική, κοινωνική και συναισθηματική ανάπτυξη, αλλά και απο άσκοπες επαναλαμβανόμενες δραστηριότητες που χαρακτηρίζουν το άτομο (Παπαδάτος, 2010). Ανάλογα συμπτώματα παρατηρούνται και στην περίπτωση του αυτισμού, αφού το άτομο εκτελεί παρόμοιες επαναλαμβανόμενες στερεοτυπικές κινήσεις καθώς και εμφανίζει δυσκολία στην επικοινωνία με κύριο χαρακτηριστικό την απουσία βλεμματικής επαφής (Συνοδινού, 2007). Οι δυσκολίες που αντιμετωπίζει το παιδί με αυτισμό στην επικοινωνία έχουν επίπτωση και στην συμπεριφορά του στο σχολικό πλαίσιο και κυρίως στις κοινωνικές τους συναναστροφές με βασικό σύμπτωμα την απρόκλητη επιθετικότητα προς τους άλλους (Βάρβογλη, 2005). Στην συγκεκριμένη έρευνα πραγματοποιείται ποιοτική περιγραφική προσέγγιση μέσω της συμμετοχικής παρατήρησης σε δείγμα τεσσάρων παιδιών σχολικής ηλικίας, ήτοι, δύο αγοριών και δύο κοριτσιών απο οκτώ (8) έως δεκατεσσάρων (14) ετών. Στόχος αυτής συνίσταται στη συγκριτική διερεύνηση της επιθετικής συμπεριφοράς των παιδιών με αυτισμό, προκειμένου να καταστεί κατανοήσιμη και ορθά αντιμετωπίσιμη απο την επιστημονική κοινότητα, αλλα και απο τους δασκάλους και τους γονείς. Όπως φάνηκε από την ανάλυση της κλείδας παρατήρησης παρατηρήθηκε διαφορά στην επιθετική συμπεριφορά σε σχέση με την ανεξάρτητη μεταβλητή φύλο, όπως και σε σχέση με το ηλικιακό εύρος. </p>
Η διδασκαλία των ξένων γλωσσών στα μονοπάτια της τέχνης. Παρουσίαση εφαρμογής στο μάθημα των γαλλικών.
Authors: Σάμιος Γουσταύος 
Published: October 2016

<p>Στη ξενόγλωσση εκπαίδευση η κουλτούρα σημασιολογείται ως <em>ανθρωπολογική κουλτούρα</em> και ως <em>καλλιεργημένη κουλτούρα</em>. Η έμφαση δίνεται στην πρώτη όπου προσεγγίζεται ο πολιτισμός υπό την εθνολογική του διάσταση. Η <em>καλλιεργημένη κουλτούρα</em> στην οποία εδράζονται όλες οι μορφές της τέχνης ουσιαστικά δεν αξιοποιείται, γεγονός που δεν συνάδει με τον επιδιωκόμενο σκοπό της εκπαίδευσης: την ολόπλευρη ανάπτυξη της προσωπικότητας των μαθητών.<strong> </strong>Εκκινώντας από τις θέσεις αυτές, την περίοδο 2012-2013 οι μαθητές της Β΄ και Γ΄ Τάξης του Γυμνασίου Παναγιάς Διασέλλου Άρτας εκπόνησαν το διαθεματικό πρόγραμμα ‘25 Μικροί Δημιουργοί «βλέπουν» γαλλική ποίηση’. Σκοπός της εισήγησης είναι μέσα από την παρουσίαση του εν λόγω προγράμματος να αναδειχθεί η σπουδαιότητα της υλοποίησης βιωματικών δράσεων που θεμελιώνονται στην αλληλεπίδραση μεταξύ των καλών τεχνών και του λόγου ως εργαλείων ποιοτικής μάθησης.</p><p><strong> </strong></p>
Διερευνώντας την παραβατικότητα των μαθητών/τριών από τη σκοπιά των γονέων
Authors: Τριανταφύλλου Ευπραξία , Βοιυδούρη Αγγελική , Κοντοσώρου Αναστασία 
Published: October 2016

<p>Η παρούσα εργασία πραγματεύεται το θέμα της παραβατικότητας των ανηλίκων στην Ελλάδα. Η νεανική παραβατικότητα αποτελεί ένα μείζον κοινωνικό ζήτημα που πλέον έχει πάρει τεράστιες διαστάσεις και στην χώρα μας. Τα ερωτήματα που εγείρονται είναι πολλά. Ωστόσο, η παρούσα έρευνα έδωσε βαρύτητα στην εξέταση του φαινομένου από τη σκοπιά των γονέων. Ειδικότερα, η εργασία μας εστιάζει και αναλύει: α) τις μορφές παραβατικότητας που παρουσιάζουν τα μικρά παιδιά εντός και εκτός της σχολικής μονάδας, β) τα αίτια της αύξησης των κρουσμάτων της παραβατικότητας και ειδικότερα του σχολικού εκφοβισμού, καθώς και γ) τη στάση των γονέων ως προς την πρόληψη και την αντιμετώπιση των προβλημάτων αυτών. </p>