bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Surface Click Reactions to Localized Surface Plasmon Resonance (LSPR) Biosensing
Authors: Yaakov Noga , Chaikin Yulia , Wexselblatt Ezequiel , Tor Yitzhak , Vaskevich Alexander , Rubinstein Israel 
Published: May 2017

AbstractLocalized surface plasmon resonance (LSPR) spectroscopy is an effective tool for sensitive, affordable, and label‐free biosensing. LSPR transducers based on nanoparticulate Au films have been applied to biosensing of receptor–analyte interactions, employing primarily thiolated receptors for constructing biorecognition interfaces on nanostructured Au surfaces. This popular method suffers from a major drawback, that is, the need to prepare a thiolated receptor for each system used, which is typically synthetically complex and time‐consuming. Herein, we present an alternative approach based on the click reaction between azide and terminal alkyne, which avoids the need to synthesize thiol‐derivatized receptors and is applicable to the heterogeneous morphology of LSPR transducers. The receptors are tethered with an alkyne group, which is considerably simpler than thiolation, while producing a stable product. The transducer surface is modified with a layer of a commercial long‐chain thiol–azide molecule, then clicked with an alkyne‐dertivatized receptor to produce the biorecognition interface. This method is employed for immobilization of four different alkyne‐bearing receptor molecules on Au nano‐island film based LSPR transducers, followed by testing of their performance in biorecognition of specific analytes using LSPR and FTIR spectroscopies. The results establish the usefulness of click chemistry for the preparation of biorecognition interfaces on nanostructured LSPR transducers.
Multivalency To Inhibit and Discriminate Hexosaminidases
Authors: Alvarez‐Dorta Dimitri , King Dustin T. , Legigan Thibaut , Ide Daisuke , Adachi Isao , Deniaud David , Désiré Jérôme , Kato Atsushi , Vocadlo David , Gouin Sébastien G. , Blériot Yves 
Published: May 2017

AbstractA set of multivalent polyhydroxylated acetamidoazepanes based on ethylene glycol, glucoside, or cyclodextrin scaffolds was prepared. The compounds were assessed against plant, mammalian, and therapeutically relevant hexosaminidases. Multimerization was shown to improve the inhibitory potency with synergy, and to fine tune the selectivity profile between related hexosaminidases.
Mechanism‐Driven Approach To Develop a Mild and Versatile C−H Amidation through IrIII Catalysis
Authors: Hwang Yeongyu , Park Yoonsu , Chang Sukbok 
Published: May 2017

AbstractDescribed herein is a mechanism‐based approach to develop a versatile C−H amidation protocol under IrIII catalysis. Reaction kinetics of a key C−N coupling step with acyl azide and 1,4,2‐dioxazol‐5‐one led us to conclude that dioxazolones are much more efficient in mediating the formation of a carbon−nitrogen bond from an iridacyclic intermediate. Computational analysis revealed that the origin of higher reactivity is asynchronous decarboxylation motion, which may facilitate the formation of Ir‐imido species. Importantly, stoichiometric reactivity was successfully translated into catalytic activity with a broad range of substrates (18 different types), many of which are regarded as challenging to functionalize. Application of the new method enables late‐stage functionalization of drug molecules.
Pedological investigation of an early Bronze Age site in southern Italy
Authors: Vingiani Simona , Minieri Luciana , Albore Livadie Claude , Di Vito Mauro A. , Terribile Fabio 
Published: May 2017

AbstractArchaeological excavations over the last 40 years in Campania (southern Italy) confirm intense human occupation since the early Bronze Age (EBA). A pedological analysis of a ∼9 m deep pedostratigraphic sequence at Palma Campania (Naples) provides insights into fertility, rates of soil formation, and environmental conditions over the past 10 kyr. Fourteen volcanic soils formed in parent materials from Vesuvius and Campi Flegrei volcanic eruptions were analyzed. Results show that soils differ markedly in terms of thickness, andic properties, chemical fertility, and degree of development. Chemical properties, along with specific soil micromorphological features (such as silt coatings, laminar structure, iron segregations), are interpreted in terms of pedogenetic processes and used to reconstruct past environmental conditions. The degree of soil development, evaluated on the basis of organic matter content and some andic properties, proved more indicative of climate and geomorphological stability than duration of pedogenesis. Since the excavation also revealed an extensive EBA paleosurface and soil, targeted analyses were carried out to gain a better understanding of the impact of human activities and domestic animals on soil properties.
Müller glial cell reactivation in Xenopus models of retinal degeneration
Authors: Langhe Rahul , Chesneau Albert , Colozza Gabriele , Hidalgo Magdalena , Ail Divya , Locker Morgane , Perron Muriel 
Published: May 2017

AbstractA striking aspect of tissue regeneration is its uneven distribution among different animal classes, both in terms of modalities and efficiency. The retina does not escape the rule, exhibiting extraordinary self‐repair properties in anamniote species but extremely limited ones in mammals. Among cellular sources prone to contribute to retinal regeneration are Müller glial cells, which in teleosts have been known for a decade to re‐acquire a stem/progenitor state and regenerate retinal neurons following injury. As their regenerative potential was hitherto unexplored in amphibians, we tackled this issue using two Xenopus retinal injury paradigms we implemented: a mechanical needle poke injury and a transgenic model allowing for conditional photoreceptor cell ablation. These models revealed that Müller cells are indeed able to proliferate and replace lost cells following damage/degeneration in the retina. Interestingly, the extent of cell cycle re‐entry appears dependent on the age of the animal, with a refractory period in early tadpole stages. Our findings pave the way for future studies aimed at identifying the molecular cues that either sustain or constrain the recruitment of Müller glia, an issue of utmost importance to set up therapeutic strategies for eye regenerative medicine.
Multimodal connectivity‐based parcellation reveals a shell‐core dichotomy of the human nucleus accumbens
Authors: Xia Xiaoluan , Fan Lingzhong , Cheng Chen , Eickhoff Simon B. , Chen Junjie , Li Haifang , Jiang Tianzi 
Published: May 2017

AbstractThe subdifferentiation of the nucleus accumbens (NAc) has been extensively studied using neuroanatomy and histochemistry, yielding a well‐accepted dichotomic shell/core architecture that reflects dissociable roles, such as in reward and aversion, respectively. However, in vivo parcellation of these structures in humans has been rare, potentially impairing future research into the structural and functional characteristics and alterations of putative NAc subregions. Here, we used three complementary parcellation schemes based on tractography, task‐independent functional connectivity, and task‐dependent co‐activation to investigate the regional differentiation within the NAc. We found that a 2‐cluster solution with shell‐like and core‐like subdivisions provided the best description of the data and was consistent with the earlier anatomical shell/core architecture. The consensus clusters from this optimal solution, which was based on the three schemes, were used as the final parcels for the subsequent connection analyses. The resulting connectivity patterns presented inter‐hemispheric symmetry, convergence and divergence across the modalities, and, most importantly, clearly distinct patterns between the two subregions. This convergent connectivity patterns also confirmed the connections in animal models, supporting views that the two subregions could have antagonistic roles in some circumstances. Finally, the identified parcels should be helpful in further neuroimaging studies of the NAc. Hum Brain Mapp 38:3878–3898, 2017. © 2017 Wiley Periodicals, Inc.
Remembering my friends: Medial prefrontal and hippocampal contributions to the self‐reference effect on face memories in a social context
Authors: Yamawaki Rie , Nakamura Kimihiro , Aso Toshihiko , Shigemune Yayoi , Fukuyama Hidenao , Tsukiura Takashi 
Published: May 2017

AbstractMemories associated with the self are remembered more accurately than those associated with others. The memory enhancement related to the self is known as the self‐reference effect (SRE). However, little is known regarding the neural mechanisms underlying the SRE in a social context modulated by social relationships. In the present fMRI study, we investigated encoding‐related activation of face memories encoded with the self‐referential process in a social context that was manipulated by imagining a person‐to‐person relationship. Healthy young adults participated in the present study. During encoding, participants encoded unfamiliar target faces by imagining a future friendship with themselves (Self), their friends (Friend), or strangers (Other). During retrieval, participants were presented with target and distracter faces one by one, and they judged whether each face had been previously learned. In the behavioral results, target faces encoded in the Self condition were remembered more accurately than those encoded in the Other condition. fMRI results demonstrated that encoding‐related activation in the medial prefrontal cortex (mPFC) was significantly greater in the Self condition than in the Friend or Other conditions. In addition, the generalized psycho‐physiological interaction (gPPI) analysis showed that functional connectivity between activation in the hippocampus and the cortical midline structures (CMSs), including the mPFC and precuneus, was significant in the Self but not in the Other condition. These findings suggest that the SRE in a social context could be involved in the interaction between the CMS regions, which are related to the self‐referential process, and the hippocampus related to the memory process. Hum Brain Mapp 38:4256–4269, 2017. © 2017 Wiley Periodicals, Inc.
Confocal endomicroscopy in diagnosis of intestinal chronic graft‐versus‐host disease
Authors: Rieger Kathrin , Günther Ute , Erben Ulrike , Kühl Anja , Loddenkemper Christoph , Pezzutto Antonio , Siegmund Britta , Bojarski Christian 
Published: May 2017

AbstractGraft‐versus‐host disease (GvHD) is a major complication of allogeneic stem cell transplantation. High‐resolution in vivo histology of the intestine by confocal endomicroscopy (CEM) detects acute GvHD (aGvHD) with high sensitivity. This pilot study aims to evaluate the diagnostic value of CEM for intestinal chronic GvHD (cGvHD). The study included 20 patients with gastrointestinal symptoms and confirmed cGvHD in other organs as well as 20 patients with clinically suspected acute GvHD for control. Confocal endomicroscopy was performed as gastroscopy followed by sigmoidoscopy after intravenous injection of fluorescein (10%) and topical application of acriflavine (0.05%). Histopathology from H&E‐stained biopsy samples throughout the intestinal tract complemented the survey. All histological features of intestinal cGvHD were predominantly mild to moderate. Stroma fibrosis detected by standard histology (16/20 patients) was not seen by CEM. Apoptosis assessed by histology in 12/20 patients was concordant with CEM (8/12 patients). Confocal endomicroscopy revealed esophageal manifestation of cGvHD in 3 patients. For each biopsy site, CEM correlated with intestinal histology (r = 0.64). Classical histology from intestinal biopsy samples taken under CEM monitoring confirmed the final diagnosis of cGvHD. The sensitivity of CEM with 40% in cGvHD was significantly lower compared to 70% in patients with aGvHD. Confocal endomicroscopy detected acute features of cGvHD and contributed to the diagnosis of esophageal cGvHD but failed to display stroma fibrosis in vivo. Although CEM represents a useful noninvasive tool in routine diagnostic of intestinal aGvHD, the method is not sufficient to fully establish the diagnosis of cGvHD within the intestinal tract. Confocal endomicroscopy allowed acquisition of targeted biopsies in patients suspected of having cGvHD.
Retracted: Cyclin D1 Gene Silencing Promotes IL‐1β‐Induced Apoptosis in Rat Chondrocytes
Authors: Zan Peng‐Fei , Yao Jie , Wu Zhong , Yang Yong , Hu Shuo , Li Guo‐Dong 
Published: May 2017

ABSTRACTThis study investigated the effects of cyclin D1 gene silencing on cell proliferation and apoptosis of interleukin‐1β (IL‐1β)‐induced osteoarthritis (OA) chondrocytes. Chondrocytes from healthy sprague‐dawley rats were divided into blank, OA model (chondrocytes underwent IL‐1β inducement), OA trial (chondrocytes underwent IL‐1β inducement with cyclin D1‐shRNA treatment), and negative control (NC; chondrocytes underwent IL‐1β inducement and control‐shRNA treatment) groups. Cell proliferation was assessed by CCK‐8 assay, and cell cycle and apoptosis by flow cytometry. qRT‐PCR and Western blotting were performed to detect cyclin D1 and apoptosis‐related factors expression levels. Chondrocyte proliferation increased after 72–96 h after incubation. The OA trial group exhibited reduced cell proliferation at 48, 72, and 96 h after treatment. The OA model, OA trial, and NC groups all contained more cells arrested in G1 phase and had higher apoptosis rates than the blank group. Additionally, the OA trial group contained more cells arrested in G1 phase, with increased apoptosis rates compared to the OA model and NC groups. The OA model group had lowest expression of cyclin D1 whereas the blank group contained the highest among the four groups. qRT‐PCR also showed that the OA model, OA trial, and NC groups all had increased expression levels of Bax and reduced expression levels of Bcl‐2 and P53 compared to the blank group, whereby by the OA group had the most significant change. The combined evidence in our study shows that cyclin D1 gene silencing suppresses proliferation and induces apoptosis of rat chondrocytes in IL‐1β‐induced OA. J. Cell. Biochem. 119: 290–299, 2018. © 2017 Wiley Periodicals, Inc.
Visibility of focal liver lesions: Comparison between kupffer phase of CEUS with sonazoid and hepatobiliary phase of gadoxetic acid–enhanced MRI
Authors: Yang Woo Young , Park Hee Sun , Kim Young Jun , Yu Mi Hye , Jung Sung Il , Jeon Hae Jeong 
Published: May 2017

ABSTRACTBackgroundTo investigate the agreement between Kupffer phase of Sonazoid contrast‐enhanced sonography (CEUS) and hepatobiliary phase of gadoxetic acid–enhanced MRI in the evaluation of focal liver lesions (FLLs).MethodsOne hundred fifty‐four FLLs in 154 patients who underwent both Sonazoid CEUS and gadoxetic acid–enhanced liver MRI were included in this retrospective study. FLL visibility on the Kupffer‐phase images was graded as one (invisible or isoenhancing), two (vaguely visible or vaguely hypoenhancing), or three (clearly visible or clearly hypoenhancing), and that on the hepatobiliary‐phase images of MRI was graded as one (invisible or hyper/isointense), two (vaguely visible or weakly hypointense), or three (clearly visible or strongly hypointense). Pairwise comparison of lesion visibility between the two modalities was performed, and intermodality agreement was assessed.ResultsOn Kupffer‐phase CEUS, 31 (20.1%) lesions were invisible, 17 (11.1%) were vaguely visible, and 106 (68.9%) were clearly visible. On the hepatobiliary‐phase MRI, 9 (5.9%) lesions were invisible, 45 (29.2%) were vaguely visible, and 100 (64.9%) were clearly visible. Overall, lesion visibility scores were not significantly different between the two modalities (p = 0.121), but the visibility was significantly better on MRI in smaller lesions. Twenty‐eight lesions (18.2%) showed discrepancy in the visibility on CEUS and MRI, and most of the cases (89.7%) were lesions that were invisible on CEUS but visible on MRI.ConclusionsThe overall visibility of FLLs was comparable between the Kupffer phase of Sonazoid‐CEUS and the hepatobiliary‐phase images of gadoxetic acid–enhanced MRI, with a discrepancy between the two modalities in 18% of the cases. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 45:542–550, 2017
Acidic electrolysed water delays browning by destroying conformation of polyphenoloxidase
Authors: Sun Jiangping , Wang Meng , Liu Haiquan , Xie Jing , Pan Yingjie , Xu Changhua , Zhao Yong 
Published: May 2017

AbstractBACKGROUNDBrowning frequently occurs at fruits, vegetables and aquatic products during storage, and it drastically reduces the consumer's acceptability, with considerable financial loss. The objective of this paper was to investigate the effects of acidic electrolysed water (AEW) technology on polyphenoloxidase (PPO), which is an essential enzyme for browning.RESULTSAEW ice exhibited a good ability in delaying browning in shrimp. Kinetic study revealed that AEW exhibited the mixed type inhibition of PPO with a Ki value of 1.96 mmol L−1. Moreover, both the circular dichroism spectrum and Fourier transform infrared spectroscopy analyses revealed that the α‐helix in PPO decreased whereas random coil increased which indicates that PPO conformation was destroyed.CONCLUSIONThus, this paper may provide a deeper understanding of the application of AEW technology for preventing browning in the food industry. © 2017 Society of Chemical Industry
On spaces weakly ‐analytic
Authors: Ferrando Juan Carlos , Ka̧kol Jerzy , López‐Pellicer Manuel 
Published: May 2017

AbstractA subset Y of the dual closed unit ball of a Banach space E is called a Rainwater set for E if every bounded sequence of E that converges pointwise on Y converges weakly in E. In this paper, topological properties of Rainwater sets for the Banach space of the real‐valued continuous and bounded functions defined on a completely regular space X equipped with the supremum‐norm are studied. This applies to characterize the weak K‐analyticity of in terms of certain Rainwater sets for . Particularly, we show that is weakly K‐analytic if and only if there exists a Rainwater set Y for such that is both K‐analytic and angelic, where denotes the topology on of the pointwise convergence on Y. For the case when X is compact, one gets classic Talagrand's theorem. As an application we show that if X is a compact space and Y is a ‐dense subspace, then X is Talagrand compact, i.e., is K‐analytic, if and only if the space is K‐analytic.
The solution of elastostatic and dynamic problems using the boundary element method based on spherical Hankel element framework
Authors: Hamzehei Javaran S. , Shojaee S. 
Published: May 2017

SummaryIn this paper, new spherical Hankel shape functions are used to reformulate boundary element method for 2‐dimensional elastostatic and elastodynamic problems. To this end, the dual reciprocity boundary element method is reconsidered by using new spherical Hankel shape functions to approximate the state variables (displacements and tractions) of Navier's differential equation. Using enrichment of a class of radial basis functions (RBFs), called spherical Hankel RBFs hereafter, the interpolation functions of a Hankel boundary element framework has been derived. For this purpose, polynomial terms are added to the functional expansion that only uses spherical Hankel RBF in the approximation. In addition to polynomial function fields, the participation of spherical Bessel function fields has also increased robustness and efficiency in the interpolation. It is very interesting that there is no Runge phenomenon in equispaced Hankel macroelements, unlike equispaced classic Lagrange ones. Several numerical examples are provided to demonstrate the effectiveness, robustness and accuracy of the proposed Hankel shape functions and in comparison with the classic Lagrange ones, they show much more accurate and stable results.
Time‐varying input delay compensation for nonlinear systems with additive disturbance: An output feedback approach
Authors: Deng Wenxiang , Yao Jianyong , Ma Dawei 
Published: May 2017

SummaryThis paper addresses the output feedback tracking control of a class of multiple‐input and multiple‐output nonlinear systems subject to time‐varying input delay and additive bounded disturbances. Based on the backstepping design approach, an output feedback robust controller is proposed by integrating an extended state observer and a novel robust controller, which uses a desired trajectory‐based feedforward term to achieve an improved model compensation and a robust delay compensation feedback term based on the finite integral of the past control values to compensate for the time‐varying input delay. The extended state observer can simultaneously estimate the unmeasurable system states and the additive disturbances only with the output measurement and delayed control input. The proposed controller theoretically guarantees prescribed transient performance and steady‐state tracking accuracy in spite of the presence of time‐varying input delay and additive bounded disturbances based on Lyapunov stability analysis by using a Lyapunov‐Krasovskii functional. A specific study on a 2‐link robot manipulator is performed; based on the system model and the proposed design procedure, a suitable controller is developed, and comparative simulation results are obtained to demonstrate the effectiveness of the developed control scheme.
Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species
Authors: Gavrilović Milan , Erić Suzana , Marin Petar D. , Garcia-Jacas Núria , Susanna Alfonso , Janaćković Pedja 
Published: May 2017

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.
Abnormal Growth and Feeding Behavior Persist After Removal of Upper Airway Obstruction in Juvenile Rats
Authors: Assadi Mohammad H. , Shknevsky Elena , Segev Yael , Tarasiuk Ariel 
Published: May 2017

AbstractPediatric obstructive sleep-disordered breathing is associated with growth retardation, but also with obesity that has a tendency to persist following treatment. We investigated the effect of upper airways obstruction (AO) and of obstruction removal (OR) in juvenile rats on gut-derived ghrelin and related hypothalamic factors, feeding, and growth hormone (GH) homeostasis. Here, we show that after seven weeks of AO, animals gained less weight compared to controls, despite an increase in food intake due to elevated ghrelin and hypothalamic feeding factors. OR rats who had complete restoration of tracheal diameter, consumed more food due to increased ghrelin and exhibited growth retardation due to deregulation of GH homeostasis. This study is the first to show dysregulation of the hormonal axes controlling feeding behavior and growth that are not fully restored following OR. Thus, surgical treatment by itself may not be sufficient to prevent post-surgical increased food intake and growth retardation.