bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryopreservation of Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, 1853 (Decapoda, Brachyura), embryos by vitrification
Authors: Huang XiaoRong , Zhuang Ping , Feng GuangPeng , Zhao Feng , Liu JianYi , Zhang Tao , Wang Yu , Wang SiKai 
Published: November 2017

Though cryopreservation of some invertebrate embryos was successful, the cryopreservation of crustacean embryos has not yet been reported. On the basis of previous study, a single vitrifying cocktail (Code A: 30% propanediol and 20% dimethyl formamide) was selected as a cryoprotectant from six kinds of vitrifying solutions to cryopreserve E. sinensis embryos. The cleavage stage, the gastrula, the pre-nauplius, and the first zoea stage embryos were serially acclimated in 25, 33, 50, 67 and 100% vitrifying solution A for 6 min. Embryos were acclimated for 1 min on the 20 cm liquid nitrogen (LN2) layer, and then plunged into liquid nitrogen. After storage for several minutes in LN2, straws containing frozen embryos were acclimated for 1 min on the 20 cm liquid nitrogen (LN2) layer, then quickly removed from LN2. For thawing, straws were immersed quickly for 1 min in a water bath at 37°C, then carefully washed with 0.25 mol/l sucrose and were then incubated with 15 ppt seawater in a Petri dish at 25°C. The survival rate in the vitrifying solution differed for embryos in different stages of development, and the survival rate of different stage embryos declined with the increase of acclimation time in code A vitrifying solution. The survival rate of pre-nauplius stage embryos did not significantly differ when the embryos were washed for 5, 10, 15, or 20 min with 0.25 mol/l sucrose after thawing. There was no survival after either cleavage stage embryos, or gastrula stage embryos were frozen. Eight pre-nauplius stage embryos survived and the survival rate was 9.3 ± 2.5%, but subsequently the embryos died at the fourth day. Seven first zoea stage embryos survived, and the survival rate was 11.3 ± 3.6%, one frozen-thawed embryo hatched at the seventh day. We conclude that cryopreservation of E. sinensis embryos by vitrification is feasible.
Population structure and fecundity of two species of grapsid crabs (Brachyura, Grapsidae) that inhabit the oyster reefs of western Taiwan
Authors: Chen Kun-Neng , Hsu Jung-Ting , Ueng Yih-Tsong 
Published: November 2017

This study on carcinological aspects of oyster farming was carried out at the coast of Yunlin County, Taiwan, for a period of 25 months from July 2012 to July 2014. The space formed by the clustering of the oyster shells provides a habitat for at least eight species of crabs. Nanosesarma minutum was the dominant species (73.7% or ) with an average of 59.5 ± 30.6 individuals/string/month, while the average monthly rate of ovigerous females was 12.6 ± 13.0%, and the average number of eggs per berried female was 1510.8 ± 1018.7 eggs/individual. Hemigrapsus penicillatus was the second dominant species (23.9% or ) with an average of 17.4 ± 17.1 individuals/string/month; here, the average monthly rate of ovigerous female crabs was 4.6 ± 9.0, and the average number of eggs of a berried female was 3073.4 ± 2167.5 eggs/individual.
Validation of Essential Acoustic Parameters for Highly Urgent In-Vehicle Collision Warnings
Authors: Lewis Bridget A. , Eisert Jesse L. , Baldwin Carryl L. 
Published: November 2017

Objective The aim of this study was to validate the importance of key acoustic criteria for use as in-vehicle forward collision warning (FCW) systems. Background Despite recent advances in vehicle safety, automobile crashes remain one of the leading causes of death. As automation allows for more control of noncritical functions by the vehicle, the potential for disengagement and distraction from the driving task also increases. It is, therefore, as important as ever that in-vehicle safety-critical interfaces are intuitive and unambiguous, promoting effective collision avoidance responses upon first exposure even under divided-attention conditions. Method The current study used a driving simulator to assess the effectiveness of two warnings, one that met all essential acoustic parameters, one that met only some essential parameters, and a no-warning control in the context of a lead vehicle–following task in conjunction with a cognitive distractor task and collision event. Results Participants receiving an FCW comprising five essential acoustic components had improved collision avoidance responses relative to a no-warning condition and an FCW missing essential elements on their first exposure. Responses to a consistently good warning (GMU Prime) improved with subsequent exposures, whereas continued exposure to the less optimal FCW (GMU Sub-Prime) resulted in poorer performance even relative to receiving no warning at all. Conclusions This study provides support for previous warning design studies and for the validity of five key acoustic parameters essential for the design of effective in-vehicle FCWs. Application Results from this study have implications for the design of auditory FCWs and in-vehicle display design.
Radical Political Unionism as a Strategy for Revitalization in Argentina
Authors: D’Urso Lucila , Longo Julieta 
Published: November 2017

The grassroots union experiment undertaken at the Lear automotive parts factory in Argentina can be seen as a paradigmatic struggle for an understanding of the relationship between unionism and politics. The Lear case reveals that the distinctiveness of radical political unionism lies in the democratic elements of its decision making and its appeal to direct action, its construction of alliances with other social organizations, its linkage of economic demands with broader political objectives, its identification of the management, the state, and the union bureaucracy as adversaries, and its transmission of a leftist political culture. La experiencia sindical de la fábrica de autopartes Lear en Argentina puede ser analizada como un conflicto paradigmático para comprender la relación entre sindicalismo y política. El caso de Lear revela que el carácter distintivo del sindicalismo político radical se encuentra en los mecanismos democráticos de toma de decisiones y en la apelación a medidas de acción directa, la construcción de alianzas con otras organizaciones sociales, la vinculación de las demandas económicas con objetivos políticos más amplios, la identificación de la empresa, el Estado y la burocracia sindical como adversarios y la transmisión de una cultura política de izquierda.
Measuring the quality of end-of-life care: Development, testing, and cultural validation of the Danish version of Views of Informal Carers’ Evaluation of Services–Short Form
Authors: Ross Lone , Neergaard Mette Asbjoern , Petersen Morten Aagaard , Groenvold Mogens 
Published: November 2017

Background: The perspectives of patients and relatives are important in the improvement of the quality of health care. However, the quality of end-of-life care has not been systematically evaluated in Scandinavia. Aim: To develop or adapt and subsequently validate a questionnaire assessing the quality of end-of-life care in Denmark. The questionnaire was intended for bereaved relatives in order to assess the quality of care in the last 3 months of the patient’s life and the circumstances surrounding death. Design and data sources: Based on the literature and interviews with 15 bereaved relatives and 17 healthcare professionals, relevant topics to include in a questionnaire were identified. The topics were prioritized by 100 bereaved relatives and subsequently compared to existing questionnaires. The chosen questionnaire was tested by cognitive interviews with 36 bereaved relatives. Results: Most of the important topics were covered by the Views of Informal Carers’ Evaluation of Services–Short Form, but not all Danish settings (e.g. home care by a palliative team) were covered. These settings were added to the Views of Informal Carers’ Evaluation of Services–Short Form, and a few adaptations were made before a Danish version of the Views of Informal Carers’ Evaluation of Services–Short Form was tested by cognitive interviews. This cultural validation showed that the slightly adapted Danish version was perceived as relevant, understandable, and acceptable. Furthermore, the cognitive interviews gave insight in the comprehension and interpretation of Views of Informal Carers’ Evaluation of Services–Short Form items. Conclusion: With a few adaptations, the British Views of Informal Carers’ Evaluation of Services–Short Form was relevant in a Danish setting.
Caring for Brain Tumour Patients: Psychological Reactions After Bereavement
Authors: Petruzzi Alessandra , Finocchiaro Claudia Yvonne , Rainoldi Laura , Silvani Antonio , Farinotti Mariangela , Salmaggi Andrea , Sarno Lucio , Lamperti Elena 
Published: November 2017

Aim The aim of this study was to describe the changes in quality of life and in levels of anxiety and depression experienced by caregivers of patients with brain tumour 18 months after their bereavement. Methods This longitudinal study employed data from two time points: time 1, during the hospital stay of the caregiver's loved one; and time 2, approximately 18 months after the death of the patient. A total of 51 caregivers agreed to participate in the study at both time points. We used the Hospital Anxiety and Depression Scale and the 36-Item Short Form Health Survey; we compared the data obtained at the two time points using the paired-samples t-test. Results Caring for someone with a brain tumour had a greater impact on the caregivers’ mental health than on their physical well-being. At time 2, the caregivers’ levels of anxiety and depression and psychological burden were decreased, indicating that they were in a better emotional state than they had been in time 1. However, the mean values in the depression, vitality, and mental health subscales were lower than those in the normative data, indicating that these caregivers had a worse psychological status than members of the normative group. Conclusion Our study underscores the necessity of supporting caregivers and monitoring their suffering levels; such suffering can compromise their social and work lives, not only during the disease trajectory but also in bereavement. Providing psychological and emotional support for caregivers of patients with brain tumour during both periods could lessen the suffering and unhappiness of these caregivers.
A United Kingdom Register study of in-hospital outcomes of patients receiving extracorporeal carbon dioxide removal
Authors: Cummins Carole , Bentley Andrew , McAuley Daniel F , McNamee James J , Patrick Hannah , Barrett Nicholas A 
Published: November 2017

Introduction Extracorporeal membrane carbon dioxide removal may have a role in treatment of patients with hypercapnic respiratory failure and refractory hypoxaemia and/or hypercapnia. Methods We report on the use, outcomes and complications in United Kingdom intensive care units reporting patients on the Extracorporal Life Support Organisation register. Results Of 60 patients, 42 (70%) had primarily hypoxic respiratory failure and 18 (30%) primarily hypercapnic respiratory failure. Use of veno-venous procedures increased compared to arterio-venous procedures. Following extracorporeal membrane carbon dioxide removal, ventilatory and blood gas parameters improved at 24 h. Twenty-seven (45%) of patients died before ICU discharge, while 27 (45%) of patients were discharged alive. The most common complications related to thrombosis or haemorrhage. Discussion There is limited use of extracorporeal membrane carbon dioxide removal in UK clinical practice and outcomes reflect variability in indications and the technology used. Usage is likely to increase with the availability of new, simpler, technology. Further high quality evidence is needed.
Effects of a museum-based social prescription intervention on quantitative measures of psychological wellbeing in older adults
Authors: Thomson Linda J , Lockyer Bridget , Camic Paul M , Chatterjee Helen J 
Published: November 2017

Aims: To assess psychological wellbeing in a novel social prescription intervention for older adults called Museums on Prescription and to explore the extent of change over time in six self-rated emotions (‘absorbed’, ‘active’, ‘cheerful’, ‘encouraged’, ‘enlightened’ and ‘inspired’). Methods: Participants ( n = 115) aged 65–94 years were referred to museum-based programmes comprising 10 weekly sessions, by healthcare and third sector organisations using inclusion criteria (e.g. socially isolated, able to give informed consent, not in employment, not regularly attending social or cultural activities) and exclusion criteria (e.g. unable to travel to the museum, unable to function in a group situation, unlikely to be able to attend all sessions, unable to take part in interviews and complete questionnaires). In a within-participants’ design, the Museum Wellbeing Measure for Older Adults (MWM-OA) was administered pre-post session at start-, mid- and end-programme. A total of 12 programmes, facilitated by museum staff and volunteers, were conducted in seven museums in central London and across Kent. In addition to the quantitative measures, participants, carers where present, museum staff and researchers kept weekly diaries following guideline questions and took part in end-programme in-depth interviews. Results: Multivariate analyses of variance showed significant participant improvements in all six MWM-OA emotions, pre-post session at start-, mid- and end-programme. Two emotions, ‘absorbed’ and ‘enlightened’, increased pre-post session disproportionately to the others; ‘cheerful’ attained the highest pre-post session scores whereas ‘active’ was consistently lowest. Conclusion: Museums can be instrumental in offering museum-based programmes for older adults to improve psychological wellbeing over time. Participants in the study experienced a sense of privilege, valued the opportunity to liaise with curators, visit parts of the museum closed to the public and handle objects normally behind glass. Participants appreciated opportunities afforded by creative and co-productive activities to acquire learning and skills, and get to know new people in a different context.
Knockdown of SOX2OT inhibits the malignant biological behaviors of glioblastoma stem cells via up-regulating the expression of miR-194-5p and miR-122
Authors: Su Rui , Cao Shuo , Ma Jun , Liu Yunhui , Liu Xiaobai , Zheng Jian , Chen Jiajia , Liu Libo , Cai Heng , Li Zhen , Zhao Lini , He Qianru , Xue Yixue 
Published: November 2017

Abstract
Background
Accumulating evidence has highlighted the potential role of long non-coding RNAs (lncRNAs) in the biological behaviors of glioblastoma stem cells (GSCs). Here, we elucidated the function and possible molecular mechanisms of the effect of lncRNA-SOX2OT on the biological behaviors of GSCs.

Results
Real-time PCR demonstrated that SOX2OT expression was up-regulated in glioma tissues and GSCs. Knockdown of SOX2OT inhibited the proliferation, migration and invasion of GSCs, and promoted GSCs apoptosis. MiR-194-5p and miR-122 were down-regulated in human glioma tissues and GSCs, and miR-194-5p and miR-122 respectively exerted tumor-suppressive functions by inhibiting the proliferation, migration and invasion of GSCs, while promoting GSCs apoptosis. Knockdown of SOX2OT significantly increased the expression of miR-194-5p and miR-122 in GSCs. Dual-luciferase reporter assay revealed that SOX2OT bound to both miR-194-5p and miR-122. SOX3 and TDGF-1 were up-regulated in human glioma tissues and GSCs. Knockdown of SOX3 inhibited the proliferation, migration and invasion of GSCs, promoted GSCs apoptosis, and decreased TDGF-1 mRNA and protein expression through direct binding to the TDGF-1 promoter. Over-expression of miR-194-5p and miR-122 decreased the mRNA and protein expression of SOX3 by targeting its 3’UTR. Knockdown of TDGF-1 inhibited the proliferation, migration and invasion of GSCs, promoted GSCs apoptosis, and inhibited the JAK/STAT signaling pathway. Furthermore, SOX3 knockdown also inhibited the SOX2OT expression through direct binding to the SOX2OT promoter and formed a positive feedback loop.

Conclusion
This study is the first to demonstrate that the SOX2OT-miR-194-5p/miR-122-SOX3-TDGF-1 pathway forms a positive feedback loop and regulates the biological behaviors of GSCs, and these findings might provide a novel strategy for glioma treatment.
Hybrid steepest iterative algorithm for a hierarchical fixed point problem
Authors: Husain Shamshad , Singh Nisha 
Published: November 2017

AbstractThe purpose of this work is to introduce and study an iterative method to approximate solutions of a hierarchical fixed point problem and a variational inequality problem involving a finite family of nonexpansive mappings on a real Hilbert space. Further, we prove that the sequence generated by the proposed iterative method converges to a solution of the hierarchical fixed point problem for a finite family of nonexpansive mappings which is the unique solution of the variational inequality problem. The results presented in this paper are the extension and generalization of some previously known results in this area. An example which satisfies all the conditions of the iterative method and the convergence result is given.
An evaluation of the predictive validity of the URICA and ANSOCQ scales for weight gain in adults with AN in an outpatient eating disorders program: a prospective cohort study
Authors: Green Jessica , Phillipou Andrea , Castle David , Cistullo Leonardo , Newton Richard 
Published: November 2017

Abstract
Background
The Transtheoretical Model (TTM) which focuses on stage of change has been the main conceptual model used in understanding the lack of motivation to change in patients with Anorexia Nervosa (AN). Whilst there is evidence to support the prognostic value of the TTM in AN, this evidence base sufferers from limitations including limited studies in adults and none in outpatient populations. The primary aim of this study was to clarify whether readiness to change, as measured by the University Rhode Island Change Assessment Scale (URICA) and the Anorexia Nervosa Stages of Change Questionnaire (ANSOCQ) could predict weight gain in adults with AN following treatment in an outpatient setting.

Methods
This was a prospective cohort analysis, which selectively used data from an existing clinical database at an outpatient eating disorders service. 119 patients met eligibility criteria and were included in this study. This included all adult patients who had a diagnosis of AN and were assessed, but not necessarily treated at the outpatient eating disorders program (Group 1). A subgroup of 63 patients (Group 2) was also analysed which only included patients who had received treatment at the program. Baseline measures included the URICA score, the ANSOCQ score, the Eating Disorders Examination Questionnaire (EDE-Q) and body mass index (BMI). BMI was also measured on discharge.

Results
The URICA scale had poor predictive validity for weight gain (r = 0.05, p = 0.725). The ANSOCQ had moderate predictive validity (Pearson’s r = 0.57, p = 0.007), and accounted for 32.7% of variance in weight gain. The URICA and ANSOCQ were moderately correlated in both groups. The URICA was moderately predictive of symptom severity, measured by the EDE-Q in both groups. The ANSOCQ was moderately correlated with the EDE-Q scores in both Groups 1 and 2.

Conclusions
To the authors’ knowledge this is the only study evaluating stage of change, in an adult outpatient population with AN. The findings of this study suggest that while both the URICA and ANSOCQ were associated with eating disorder symptom severity, only the ANSOCQ was able to predict weight gain in outpatients with AN suggesting its greater utility in this context.
Rheonomic phenomenon shrinkage of holes drilled in fibreglass and carbon fibre-reinforced polymer composites
Authors: Dudarev Aleksandr , Volegov Konstantin , Kurzanov Georgiy 
Published: November 2017

Abstract
Background
The research is devoted to machining polymer composite materials and reflects a non-trivial, accuracy-affecting phenomenon that occurs while drilling polymer composite materials, i.e. reduction of holes diameter over time after processing.

Methods
The article contains experimental data obtained in the research of rheonomic shrinkage of holes after drilling various grades of polymer composite materials. The research involved using different types of tools (high-performance Ham solid carbide drill bits and diamond drill bits). The tests were carried out combining different parameters of drilling modes. We adopted the range of spindle speed change n = 10,000-20,000 rev/min and tool feed range Sm = 50-300 mm/min. Measurements of shrinkage were performed with the help of ATOS III Triple Scan XL scanning system immediately after treatment and in 48 h thereafter. Based on the scanned polygonal mesh of each hole, three-dimensional models of holes drilled in the samples were built. Then, these models were verified against nominal drillings using GOM Inspect software. In this way, the values of rheonomic shrinkage were obtained. Verification was carried out according to Gaussian method of measurements by coincidence using variation variables sigma (Gaussian best-fit 3 sigma).

Results
Shrinkage of holes does not take place in structural materials. However, in polymer composite materials, shrinkage of the drilled hole of 10 mm nominal diameter may reach reduction in diameter of 0.02-0.03 mm during a period of 48 h and depends on the grade of the polymer composite material. Observations have shown that shrinkage also depends on machining mode parameters.

Conclusions
We have developed two hypotheses to explain the influence of processing mode parameters on rheonomic shrinkage: the first one relates to the rate of processing, the second – to the force factors.
Volatility Uncertainty, Time Decay, and Option Bid-Ask Spreads in an Incomplete Market
Authors: Hsieh PeiLin , Jarrow Robert 
Published: November 2017

This paper documents the fact that in options markets, the (percentage) implied volatility bid-ask spread increases at an increasing rate as the option’s maturity date approaches. To explain this stylized fact, this paper provides a market microstructure model for the bid-ask spread in options markets. We first construct a static equilibrium model to illustrate the aforementioned phenomenon where risk averse and competitive option market makers quote bid and ask prices to minimize their inventory risk in an incomplete market with both directional and volatility risk. We extend this model to multiperiods and show that the same phenomenon occurs there as well. Two new implications are generated: a volatility level effect and a volatility variance effect. These implications are empirically tested, and the empirical results confirm the model’s validity. Finally, we document the importance of detrending the maturity effect by showing that the detrended percentage volatility spread explains future jump intensities better than the original percentage volatility spread. The online appendix is available at https://doi.org/10.1287/mnsc.2017.2867 . This paper was accepted by Neng Wang, finance.