bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yttrium-90-doped metal-organic frameworks (MOFs) for low-dose rate intratumoral radiotherapy
Authors: Qi Xiaoli , Fedotova Anzhelika , Yu Zhihao , Polyanskaya Anna , Shen Ningfei , Egorova Bayirta , Bagrov Dmitry , Slastnikova Tatiana , Rosenkranz Andrey , Patriarche Gilles , Nevolin Yurii , Permyakova Anastasia , Fedotov Stanislav , Lepoitevin Mathilde , Kalmykov Stepan , Serre Christian , Durymanov Mikhail 
Published: September 2024

AbstractBrachytherapy, or intratumoral radiation therapy, is a highly effective treatment option for localized tumors. Herein, we engineered injectable and biodegradable metal-organic frameworks (MOFs) to deliver the therapeutic radioisotope yttrium-90 (90Y). Particles of bimetallic MIL-100(Fe,Y) and Y-BTC, doped with90Y and88Y, were synthesized in a single step and retained radioyttrium in various buffer solutions. Tumor injectability and radioisotope retention were evaluated using tumor-bearing mice.In vivoanalysis and calculations showed that radiolabeled MIL-100(Fe,Y) emitted more than 38% of its radioactivity, while Y-BTC emitted greater than 75% of its radioactivity, through 7 days at the tumor site upon intratumoral injection, without significant yttrium accumulation in off-target tissues. The anticancer effects of MIL-100(Fe,Y,90Y) and90Y,Y-BTC particles were assessed using 3D multicellular tumor spheroids and a tumor-bearing mouse model, respectively.90Y-doped MIL-100(Fe,Y) particles penetrated A549 tumor spheroids and caused superior cytotoxic effects compared to non-radioactive particles or90YCl3, added at the same dose. Brachytherapy with90Y-doped Y-BTC MOFs induced inhibition of B16F1 melanoma tumor growth and resulted in an increased median survival of 8.5 days compared to 4.5 days in untreated mice. This study exhibits the feasibility to prepare radioactive90Y-containing biodegradable, non-toxic MOF particles that are advantageous for low-dose rate internal radiotherapy.
Heterogeneous responses to embryonic critical period perturbations among different components of theDrosophilalarval locomotor circuit
Authors: Krick Niklas , Davies Jacob , Coulson Bramwell , Sobrido-Cameán Daniel , Miller Michael , Oswald Matthew C. W. , Zarin Aref A. , Baines Richard , Landgraf Matthias 
Published: September 2024

AbstractAs developing neural circuits become functional, they undergo a phase of heightened plasticity in response to intrinsic and/or extrinsic stimuli. These developmental windows are termed critical periods (CPs), because perturbations during the CP can lead to lasting and significant change in subsequent development, such as sub-optimal and/or unstable networks. By contrast, the same manipulations before or after the CP does not create lasting changes. Here, we have used theDrosophilalarval locomotor network to study how different identified, connected elements respond to a CP perturbation, from pre-motor interneuron to motoneuron, to neuromuscular junction. Using heat stress as an ecologically relevant stimulus, we show that increasing temperature causes increased network activity that, when applied during the CP, leads to larvae that crawl more slowly and that require longer to recover from electroshock-induced seizures, indicative of decreased network stability. Within the central nervous system, we find CP perturbation leads to pre-motor interneurons delivering increased synaptic drive to motoneurons, which in turn display reduced excitability. The peripheral neuromuscular junction, on the other hand, maintains normal synaptic transmission, despite significant structural changes of synaptic terminal overgrowth and altered postsynaptic receptor field composition. Overall, our data demonstrate that different connected elements within a network respond differentially to a CP perturbation. Our results suggest an underlying sequence, or hierarchy, of network adjustment during developmental CPs, and present the larval locomotor network as a highly tractable experimental model system with which to study CP biology.
Thorax temperature and niche characteristics as predictors of abundance of Amazonian Odonata
Authors: Calvão Lenize Batista , Faria Ana Paula J. , de Paiva Carina Kaory Sasahara , Oliveira-Junior José Max Barbosa , Muzón Javier , Córdoba-Aguillar Alex , Juen Leandro 
Published: September 2024

AbstractEnvironmental architecture and body temperature drive the distribution of ectothermic species, especially those with specific ecophysiological requirements or narrow ecological niches. In this study, we evaluated the connection between thorax temperature and niche specialization concerning the abundance and species contribution to the beta diversity of adult Odonata in Amazonian streams, employing the Species Contribution to Beta Diversity (SCBD). Our hypotheses were (i) Odonata species’ thorax temperature is positively correlated with both morphology (thorax width) and air temperature, and (ii) the thorax temperature of the Odonata assemblage serves as a more influential predictor than niche specialization in determining species abundance and SCBD. We sampled 46 streams in an anthropized landscape in the Northeastern and Southeastern regions of Pará state, Brazil. Notably, niche breadth emerged as the variable influencing the abundance and SCBD of the Odonata assemblage. Niche position is a predictor for Odonata SCBD and not suborders, and predictor for abundance, except for Anisoptera. Both suborders exhibited a negative relationship between abundance and thoracic temperature. In summary, our results underscore the necessity of considering both niche and ecophysiological predictors to comprehensively assess the Odonata assemblage in Amazonian streams. This holistic approach has implications for conservation efforts and bioassessment practices, offering valuable insights into the collective response of Odonata as a group.
The digestion time for salmon louse (Lepeoptheirus salmonis) in lumpfish (Cyclopterus lumpus)in relation to freshness, developmental stage, and temperature
Authors: Eliasen Kirstin , Østerø Sandra L. , Johannesen Tróndur T. , Patursson Esbern J. , Jacobsen Ása , Mortensen Agnes M. , Nolsøe Marner , Johannesen Ása 
Published: September 2024

AbstractSea lice infestations cause significant economic losses in the Atlantic salmon aquaculture industry. To biologically control sea lice at farming sites, cleaner fish such as lumpfish are employed. However, the efficacy of lumpfish is under constant debate, primarily due to limited knowledge of digestion times, which makes it difficult to interpret the number of salmon lice found in the stomach contents of dissected lumpfish.The aim of this study was to provide quantitative estimates of the degradation of salmon lice over a period of 12 days. After an acclimation period of approximately one week, batches of eight lumpfish (average weight 94.3 g, SD ± 33.2) were fed salmon lice and arranged in tanks.Each batch received six large mobile lice and two adult female lice. Samplings were conducted at 24-hour intervals during the first four days and at 48-hour intervals over the remaining eight days. The experiment was conducted twice, each at a different temperature regime (6°C and 9°C), using live lice in both trials. To investigate if the freshness of the louse influenced degradation and digestion, the setup was replicated in the 9°C experiment with lice that had been stored frozen at -80°C, with an additional 12-hour sampling point for comprehensive observation.The analysis of salmon lice revealed expected digestion times of 6.8 days and 12 days for large mobile and adult female salmon lice, respectively. Temperature and lice freshness did not seem to influence digestion times, but the developmental stage of the lice did. The findings of this study can be used to estimate the cleaning efficacy of lumpfish based on the stomach contents.
Voltage imaging reveals circuit computations in the raphe underlying serotonin-mediated motor vigor learning
Authors: Kawashima Takashi , Wei Ziqiang , Haruvi Ravid , Shainer Inbal , Narayan Sujatha , Baier Herwig , Ahrens Misha B. 
Published: September 2024

AbstractAs animals adapt to new situations, neuromodulation is a potent way to alter behavior, yet mechanisms by which neuromodulatory nuclei compute during behavior are underexplored. The serotonergic raphe supports motor learning in larval zebrafish by visually detecting distance traveled during swims, encoding action effectiveness, and modulating motor vigor. We found that swimming opens a gate for visual input to cause spiking in serotonergic neurons, enabling encoding of action outcomes and filtering out learning-irrelevant visual signals. Using light-sheet microscopy, voltage sensors, and neurotransmitter/modulator sensors, we tracked millisecond-timescale neuronal input-output computations during behavior. Swim commands initially inhibited serotonergic neurons via GABA, closing the gate to spiking. Immediately after, the gate briefly opened: voltage increased consistent with post-inhibitory rebound, allowing swim-induced visual motion to evoke firing through glutamate, triggering serotonin secretion and modulating motor vigor. Ablating GABAergic neurons impaired raphe coding and motor learning. Thus, serotonergic neuromodulation arises from action-outcome coincidence detection within the raphe, suggesting the existence of similarly fast and precise circuit computations across neuromodulatory nuclei.
Aquaporin-4 expression levels and mis-localization are inversely linked to peritumoral edema in gliomas of varying aggressiveness
Authors: Valente Onofrio , Abbrescia Pasqua , Signorile Gianluca , Messina Raffaella , de Gennaro Luigi , Bozzi Maria Teresa , Ingravallo Giuseppe , D’amati Antonio , Zimatore Domenico Sergio , Palazzo Claudia , Nicchia Grazia Paola , Frigeri Antonio , Signorelli Francesco 
Published: September 2024

ABSTRACTAquaporin-4 (AQP4) and its extended isoform, AQP4ex, are crucial for regulating brain water homeostasis. Mis-localization of these isoforms is implicated in various brain tumors, including glioblastoma multiforme (GBM).This study explores AQP4 isoform expression and localization in Pilocytic Astrocytomas (PA), a circumscribed astrocytic low-grade glioma, compared to GBM, an adult-type diffuse high grade glioma.We found significant upregulation of AQP4 and AQP4ex in PA, with notable mis-localization deviating from the typical perivascular localization seen in healthy tissue. This mis-localization mirrors the phenotype observed in AQP4ex knockout models, where impaired AQP4 localization is linked to disrupted water homeostasis and reduced waste clearance, despite overall increased AQP4 levels. Interestingly, PA shows minimal peritumoral edema and a relatively intact blood-brain barrier (BBB), with elevated phosphorylated AQP4ex (pAQP4ex) suggesting a role in stabilizing AQP4 function. In contrast, GBM exhibits reduced AQP4/AQP4ex expression, significant peritumoral edema, and BBB disruption.GFAP isoforms, GFAPκ and GFAPδ, are upregulated in PA, associated with Rosenthal fibers, indicating a stabilizing astrocytic response. GBM, however, shows generalized GFAP increase, reflecting aggressive gliosis and disrupted water homeostasis.In conclusion, both AQP4 expression levels and mis-localization are important factors influencing peritumoral edema and tumor aggressiveness in gliomas. This study positions AQP4 as a potential biomarker for glioma progression, offering insights into astrocytic function and paving the way for targeted therapies.
PREDICTION OF SINGLE NUCLEOTIDE VARIANT EFFECTS RELATED TO SEX DETERMINATION INCarica papaya (Caricaceae)
Authors: Bôas Spelta João Victor Villas , Sakamoto Tetsu 
Published: September 2024

AbstractPapaya (Carica papaya) produces one of the most consumed fruits worldwide, holding great economic importance, especially in tropical regions. Papaya trade mainly involves gynodioecious cultivars with a ratio of 1:1 or 2:1 of hermaphrodites to females. For commercial reasons and also inherent to cultivation, it is preferable to have as many hermaphrodites as possible. In general, it is still not possible to produce only hermaphrodite seeds, so the sex of the plant is usually identified by conventional methods after the first flowering of the papaya plant. This occurs about 4-6 months after planting the seedling, with the females typically being discarded at the end. To avoid wasting resources and achieve higher harvest yields, producers may also resort to molecular methods using sex markers. However, this alternative also has its limitations, including high costs. Given these challenges, many researchers have focused on studying the process of sex determination inC. papaya, but the factors directly influencing this process remain unknown. The elucidation of this mechanism is not only of great agronomic interest, but also represents a significant opportunity forC. papayato establish itself as a model organism for studying sexual chromosomes of recent evolutionary origin. Therefore, in this work, a bioinformatics strategy was used to address the topic. A genotype-phenotype association study was conducted to find possible genetic factors involved in sex determination using resequencing data from 36 individuals (24 male papaya plants and 12 hermaphrodites) obtained from public databases. The association study was preceded by a variant calling performed with BCFTOOLS, which found 75,607 variants, leaving 37,027 after filtering. Association studies were then carried out using the PLINK program with the filtered variants, and among these, 251 of the most statistically significant variants were applied to the SnpEff program for variant annotation, returning 449 effects, including 402 with a modifier level of impact, 22 with a low impact, and 25 with a moderate effect. Inferences and gene annotations were also performed using the Augustus software and BLASTP alignments with the gene sequences that had moderate effects predicted by SnpEff, as well as de novo genome assemblies of a male sample and its alignment with the hermaphrodite sex-determining region. These results were recorded and compared with previous studies in the literature. This allowed for the conclusion that the specific results obtained serve as a starting point for more robust studies to understand the molecular mechanisms of sex determination inC. papaya.
Positional information drives distinct traits in transcriptomically identified neuronal types
Authors: Shainer Inbal , Kappel Johannes M. , Laurell Eva , Donovan Joseph C. , Schneider Martin W. , Kuehn Enrico , Arnold-Ammer Irene , Stemmer Manuel , Larsch Johannes , Baier Herwig 
Published: September 2024

SummaryNeuronal phenotypic traits such as morphology, connectivity, and function are dictated, to a large extent, by a specific combination of differentially expressed genes. Clusters of neurons in transcriptomic space correspond to distinct cell types and in some cases (e. g.,C. elegansneurons1and retinal ganglion cells2–4) have been shown to share morphology and function. The zebrafish optic tectum is composed of a spatial array of neurons that transforms visual inputs into motor outputs. While the visuotopic map is continuous, subregions of the tectum are functionally specialized5,6. To uncover the cell-type architecture of the tectum, we transcriptionally profiled its neurons, revealing more than 60 cell types that are organized in distinct anatomical layers. We then measured the visual responses of thousands of tectal neurons by two-photon calcium imaging and matched them with their transcriptional profile. Furthermore, we characterized the morphologies of transcriptionally identified neurons using specific transgenic lines. Surprisingly, we found that neurons that are transcriptionally similar can diverge functionally and morphologically. Incorporating the spatial coordinates of neurons within the tectal volume revealed functionally and morphologically defined anatomical subclusters within individual transcriptomic clusters. Our findings demonstrate that extrinsic, position-dependent factors expand the phenotypic repertoire of genetically similar neurons.
Filter bank common spatial pattern and envelope-based features in multimodal EEG-fTCD brain-computer interfaces
Authors: Essam Alaa-Allah , Ibrahim Ammar , Zanqour Ashar , El-Saqa Mariam , Mohamed Sohila , Anwar Ayman , Eldeib Ayman , Akcakaya Murat , Khalaf Aya 
Published: September 2024

AbstractBrain-computer interfaces (BCIs) exploit brain activity to bypass neuromuscular control with the aim of providing alternative means of communication with the surrounding environment. Such systems can significantly improve the quality of life for patients suffering from severe motor or speech impairment. Multimodal BCIs have been introduced recently to enhance the performance of BCIs utilizing single modality. In this paper, we aim to improve the performance of multimodal BCIs combining Electroencephalography (EEG) and functional transcranial Doppler ultrasound (fTCD). The BCIs included in the study utilized two different paradigms to infer user intent including motor imagery (MI) and flickering mental rotation (MR)/word generation (WG) paradigms. Filter Bank Common Spatial Pattern (FBCSP) algorithm was used to extract features from the EEG data. Several time series features were extracted from the envelope of the fTCD signals. Wilcoxon rank sum test and linear kernel Support vector machines (SVM) were used for feature selection and classification respectively. Additionally, a probabilistic Bayesian fusion approach was used to fuse the information from EEG and fTCD modalities. Average accuracies of 94.53%, 94.9% and 96.29% were achieved for right arm MI versus baseline, left arm MI versus baseline, and right arm MI versus left arm MI respectively. Whereas average accuracies of 95.27%, 85.93% and 96.97% were achieved for MR versus baseline, WG versus baseline, and MR versus WG respectively. Our results show that EEG- fTCD BCIs with the proposed analysis techniques outperformed the multimodal EEG-fNRIS BCIs in comparison.
Changes in nucleus accumbens core translatome accompanying incubation of cocaine craving
Authors: Kawa Alex B. , Hashimoto Joel G. , Beutler Madelyn M. , Guizzetti Marina , Wolf Marina E. 
Published: September 2024

AbstractIn the ‘incubation of cocaine craving’ model of relapse, rats exhibit progressive intensification (incubation) of cue-induced craving over several weeks of forced abstinence from cocaine self-administration. The expression of incubated craving depends on plasticity of excitatory synaptic transmission in nucleus accumbens core (NAcC) medium spiny neurons (MSN). Previously, we found that the maintenance of this plasticity and the expression of incubation depends on ongoing protein translation, and the regulation of translation is altered after incubation of cocaine craving. Here we used male and female rats that express Cre recombinase in either dopamine D1 receptor- or adenosine 2a (A2a) receptor-expressing MSN to express a GFP-tagged ribosomal protein in a cell-type specific manner, enabling us to use Translating Ribosome Affinity Purification (TRAP) to isolate actively translating mRNAs from both MSN subtypes for analysis by RNA-seq. We compared rats that self-administered saline or cocaine. Saline rats were assessed on abstinence day (AD) 1, while cocaine rats were assessed on AD1 or AD40-50. For both D1-MSN and A2a-MSN, there were few differentially translated genes between saline and cocaine AD1 groups. In contrast, pronounced differences in the translatome were observed between cocaine rats on AD1 and AD40-50, and this was far more robust in D1-MSN. Notably, all comparisons revealed sex differences in translating mRNAs. Sequencing results were validated by qRT-PCR for several genes of interest. This study, the first to combine TRAP-seq, transgenic rats, and a cocaine self-administration paradigm, identifies translating mRNAs linked to incubation of cocaine craving in D1-MSN and A2a-MSN of the NAcC.
Vocal processing networks in the human and marmoset brain
Authors: Dureux Audrey , Zanini Alessandro , Menon Ravi S. , Everling Stefan 
Published: September 2024

AbstractUnderstanding the brain circuitry involved in vocal processing across species is crucial for unraveling the evolutionary roots of human communication. While previous research has pinpointed voice-sensitive regions in primates, direct cross-species comparisons using standardized protocols are limited. This study utilizes ultra-high field fMRI to explore vocal processing mechanisms in humans and marmosets. By employing voice-sensitive regions of interest (ROIs) identified via auditory localizers, we analyzed response time courses to species-specific vocalizations and non-vocal sounds using a dynamic auditory-stimulation paradigm. This approach gradually introduced sounds into white noise over 33 seconds. Results revealed that both species have responsive areas in the temporal, frontal, and cingulate cortices, with a distinct preference for vocalizations. Significant differences were found in the response time courses between vocal and non-vocal sounds, with humans displaying faster responses to vocalizations than marmosets. We also identified a shared antero-ventral auditory pathway in both species for vocal processing, originating from the superior temporal gyrus. Conversely, a posterior-dorsal pathway was more prominent in humans, whereas in marmosets, this pathway processed both sound types similarly. This comparative study sheds light on both conserved and divergent auditory pathways in primates, providing new insights into conspecific vocalization processing.
Embryonic exposure to valproic acid and neonicotinoid deteriorates the developmental GABA switch and impairs long-term potentiation in the local circuit of intermediate medial mesopallium of chick telencephalon
Authors: Matsushima Toshiya , Toji Noriyuki , Wada Kazuhiro , Shikanai Hiroki , Izumi Takeshi 
Published: September 2024

AbstractEmbryonic exposure to valproic acid (VPA) and imidacloprid (IMI, a neonicotinoid insecticide) impairs filial imprinting in hatchlings, and the deteriorating effects of VPA are mitigated by post-hatch injection of bumetanide, a blocker of the chloride intruder NKCC1. Here, we report that these exposures depolarized the reversal potential of local GABAergic transmission in the neurons of the intermediate medial mesopallium (IMM), the pallial region critical for imprinting. Furthermore, exposure increased field excitatory post-synaptic potentials in pre-tetanus recordings (fEPSPs) and impaired long-term potentiation by low-frequency tetanic stimulation (LTP). Bath-applied bumetanide rescued the impaired LTP in the VPA slices, whereas VU0463271, a blocker of the chloride extruder KCC2, suppressed LTP in the control slices, suggesting that hyperpolarizing GABA action is necessary for the potentiation of excitatory synaptic transmission. However, the transcriptional profiles of IMM slices did not support the expected increase in the NKCC1/KCC2 ratio, suggesting a potential modification of post-transcriptional processes. Instead, exposure to both VPA and IMI downregulated several transcriptional regulators (FOS, NR4A1, and NR4A2) and upregulated the RNA component of signal recognition particles (RN7SL1). As a limited set of response genes were shared, VPA and IMI could cause common neuronal malfunctions via distinct molecular cascades.