bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Saramis 4.12 and IVD 3.0 Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Mycobacteria from Solid and Liquid Culture Media
Authors: Leyer Caroline , Gregorowicz Guillaume , Mougari Faiza , Raskine Laurent , Cambau Emmanuelle , de Briel Dominique 
Published: April 2017

ABSTRACT
During the last decade, many investigators have studied matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for identification of mycobacteria. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were subjected to protein extraction and same spectra were matched against both databases. The IVD v3.0 database proved to be superior to Saramis v4.12 and its identification rates remarkably increased, from 67% to 94% for isolates grown on Middlebrook 7H10 solid medium and from 62% to 91% for isolates grown on mycobacterial growth indicator tube (MGIT) liquid medium. With this new version, IVD v3.0, MALDI-TOF MS might be integrated into routine clinical diagnostics, although molecular techniques remain mandatory in some cases.
Bounds on supersymmetric effective operators from heavy diphoton searches
Authors: Ghilencea D. M. , Lee Hyun Min 
Published: April 2017

We identify the bounds on supersymmetric effective operators beyond minimal supersymmetric models (MSSM, etc.), from heavy diphoton resonance (X) negative searches at the LHC, where X is identified with the neutral CP-even (odd), H(A) or both (mass degenerate). While MSSM-like models comply with the data, a leading effective operator of d = 6 can contribute significantly to diphoton production [Formula: see text] 1 fb, well above its MSSM value and in conflict with recent data. Both the [Formula: see text] and gg production mechanisms of H and A can contribute comparably to this. We examine the dependence of the diphoton cross-section [Formula: see text] on the values of [Formula: see text], [Formula: see text] and tan [Formula: see text], under the experimental constraints from the SM-like Higgs couplings hgg and h[Formula: see text] (due to mixing) and from the [Formula: see text] and [Formula: see text] searches. These give [Formula: see text] larger than [Formula: see text] 5 TeV for [Formula: see text] in the range 0.5–1 TeV. We show how to generate the d = 6 effective operator from microscopic (renormalizable) models. This demands the presence of vector-like states beyond the MSSM spectrum (and eventually, but not necessarily a gauge singlet), of mass near [Formula: see text] and thus outside the LHC reach.
Targeted inactivation of copper transporter Atp7b in hepatocytes causes liver steatosis and obesity in mice
Authors: Muchenditsi Abigael , Yang Haojun , Hamilton James P. , Koganti Lahari , Housseau Franck , Aronov Lisa , Fan Hongni , Pierson Hannah , Bhattacharjee Ashima , Murphy Robert , Sears Cynthia , Potter James , Wooton-Kee Clavia R. , Lutsenko Svetlana 
Published: April 2017

Copper-transporting ATPase 2 (ATP7B) is essential for mammalian copper homeostasis. Mutations in ATP7B result in copper accumulation, especially in the liver, and cause Wilson disease (WD). The major role of hepatocytes in WD pathology is firmly established. It is less certain whether the excess Cu in hepatocytes is solely responsible for development of WD. To address this issue, we generated a mouse strain for Cre-mediated deletion of Atp7b and inactivated Atp7b selectively in hepatocytes. Atp7bΔHep mice accumulate copper in the liver, have elevated urinary copper, and lack holoceruloplasmin but show no liver disease for up to 30 wk. Liver inflammation is muted and markedly delayed compared with the age-matched Atp7b−/− null mice, which show a strong type1 inflammatory response. Expression of metallothioneins is higher in Atp7bΔHep livers than in Atp7b−/− mice, suggesting better sequestration of excess copper. Characterization of purified cell populations also revealed that nonparenchymal cells in Atp7bΔHep liver maintain Atp7b expression, have normal copper balance, and remain largely quiescent. The lack of inflammation unmasked metabolic consequences of copper misbalance in hepatocytes. Atp7bΔHep animals weigh more than controls and have higher levels of liver triglycerides and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase. By 45 wk, all animals develop liver steatosis on a regular diet. Thus copper misbalance in hepatocytes dysregulates lipid metabolism, whereas development of inflammatory response in WD may depend on copper status of nonparenchymal cells. The implications of these findings for the cell-targeting WD therapies are discussed. NEW & NOTEWORTHY Targeted inactivation of copper-transporting ATPase 2 (Atp7b) in hepatocytes causes steatosis in the absence of inflammation.
Plasma microRNAs characterising patients with immune thrombo cytopenic purpura
Authors: Zuo Bin , Zhai Juping , You Lifang , Zhao Yunxiao , Yang Jianfeng , Weng Zhen , Dai Lan , Wu Qingyu , Ruan Changgeng , He Yang 
Published: April 2017

SummaryAltered microRNA (miRNA) expression has been reported in patients with immune thrombocytopenic purpura (ITP). However, the detailed expression profiling of cell-free circulating miRNAs in ITP patients has not been fully investigated. In this study, we aimed to examine plasma miRNAs in ITP patients and evaluate their diagnostic values. Plasma samples from 74 ITP patients and 58 healthy controls were obtained and allocated into discovery, validation, and therapy-response sets. Initial screen with a miRNA microarray assay identified 23 miRNAs with different levels between ITP patients and healthy controls (>1.5-fold changes; p<0.01). Subsequent quantitative real-time PCR confirmed eight up-regulated miRNAs (miR-320c, miR-642b-3p, miR-1275, miR-3141, miR-4270, miR-4499, miR-4739 and miR-6126) and three down-regulated miRNAs (miR-144–3p, miR-1281 and miR-3162–3p) in ITP patients. The levels of these circulating miRNAs varied, depending on ITP subtypes, i.e. newly-diagnosed, persistent and chronic ITP, and between treatment responders and non-responders. In receiver operator characteristic analysis, 10 miRNAs had positive diagnostic values (p<0.05) when tested individually. The diagnostic value improved when the miRNAs were analysed as a panel or together with the analysis of anti-platelet autoantibodies. Plasma miR-3162–3p levels were also found to positively correlate with platelet counts in ITP patients (r=0.338, p=0.01). Our results indicate that plasma miRNA profiles are altered in ITP patients and that the differentially expressed miRNAs may be used as biomarkers to improve the diagnosis of ITP.
NOACs for treatment of venous thromboembolism in clinical practice
Authors: Schulman Sam , Singer Daniel , Ageno Walter , Casella Ivan B. , Desch Marc , Goldhaber Samuel Z. 
Published: April 2017

SummaryRandomised controlled trials have provided important information on the efficacy and safety of the non-vitamin K antagonist oral anticoagulants (NOACs) for treatment of venous thromboembolism (VTE), leading to registration and increasing use in clinical practice. Many questions remain to be answered, and observational studies are often more suitable for answering “real-world” questions than randomised controlled trials. Patient satisfaction, quality of life, and adherence and persistence in clinical practice with the drug regimen can only be assessed with an open-label design. Evaluation of risk for long-term sequelae of the disease requires much longer follow-up than is possible in registration trials. Treatment patterns and utilisation of health care resources can be assessed from observations in the clinical practice setting. We will review published as well as currently active observational studies with NOACs in VTE, with or without a comparator anticoagulant. These studies are based on cohorts of different sizes, registries, or administrative health care databases. We will also discuss some limitations in analysis and interpretation of observational studies.
Mortality risk factors from converting off-pump coronary artery bypass to on-pump coronary artery bypass
Authors: Miao Na , Yang Feng , Du Zhongtao , Jiang Chunjing , Hao Xing , Wang Jinhong , Jiang Yu , Yang Xiaofang , Xie Haixiu , Hou Xiaotong 
Published: April 2017

Introduction: A number of large-scale retrospective studies revealed that off-pump coronary artery bypass (OPCAB) was superior to on-pump coronary artery bypass (ONCAB). The aim of the study was to investigate risk factors for mortality when OPCAB is converted to ONCAB. Methods: Patients who underwent OPCAB conversion to ONCAB at the Beijing Anzhen Hospital between January 2003 and January 2013 were assigned to the non-survivor and survivor groups. Background demographics, illness history and preoperative, intraoperative and postoperative variables were compared. Results: Of the 247 cases, 15.4% of the patients died. Patients in the non-survivor group were older and more frequently had diabetes mellitus (DM), arrhythmia, myocardial infarction (MI) in the past 30 days (all p<0.05) and MI combined with mitral regurgitation (p<0.0001); they more frequently had bigger left ventricular end-diastolic dimension (p=0.0019), greater fall in blood pressure, ventricular fibrillation for longer periods, longer conversion time and bypass graft occlusion. All patients in the non-survivor group received intra-aortic balloon pump compared to 89.5% in the survivor group and extracorporeal membrane oxygenation was more common. Left main coronary artery disease (OR=4.431, 95%CI: 2.440-8.048, p<0.0001), blood pressure decline ⩽40 mmHg (OR=0.509, 95%CI: 0.447-0.580, p<0.0001) and time for conversion to ONCAB ⩾20 min were independently associated with mortality. Rates of postoperative complications, such as renal failure, cerebral infarction or hemorrhage, MI and redo sternotomy, were higher in the non-survivor group. Conclusions: Conversion from OPCAB to ONCAB is associated with high mortality. Risk factors include left main artery disease and duration of blood pressure decline >40 min.
Persistent idiopathic facial pain
Authors: Benoliel Rafael , Gaul Charly 
Published: April 2017

Background Persistent idiopathic facial pain (PIFP) is a chronic disorder recurring daily for more than two hours per day over more than three months, in the absence of clinical neurological deficit. PIFP is the current terminology for Atypical Facial Pain and is characterized by daily or near daily pain that is initially confined but may subsequently spread. Pain cannot be attributed to any pathological process, although traumatic neuropathic mechanisms are suspected. When present intraorally, PIFP has been termed ‘Atypical Odontalgia’, and this entity is discussed in a separate article in this special issue. PIFP is often a difficult but important differential diagnosis among chronic facial pain syndromes. Aim To summarize current knowledge on diagnostic criteria, differential diagnosis, pathophysiology and management of PIFP. Methods We present a narrative review reporting current literature and personal experience. Additionally, we discuss and differentiate the common differential diagnoses associated with PIFP including traumatic trigeminal neuropathies, regional myofascial pain, atypical neurovascular pains and atypical trigeminal neuropathic pains. Results and conclusion The underlying pathophysiology in PIFP is still enigmatic, however neuropathic mechanisms may be relevant. PIFP needs interdisciplinary collaboration to rule out and manage secondary causes, psychiatric comorbidities and other facial pain syndromes, particularly trigeminal neuralgia. Burden of disease and psychiatric comorbidity screening is recommended at an early stage of disease, and should be addressed in the management plan. Future research is needed to establish clear diagnostic criteria and treatment strategies based on clinical findings and individual pathophysiology.
Adenovirus-mediated CD40L gene transfer increases Teffector/Tregulatory cell ratio and upregulates death receptors in metastatic melanoma patients
Authors: Schiza A. , Wenthe J. , Mangsbo S. , Eriksson E. , Nilsson Anders , Tötterman T. H. , Loskog A. , Ullenhag G. 
Published: April 2017

Abstract
Background and aims
Malignant melanoma is an aggressive tumor sensitive for immunotherapy such as checkpoint blockade antibodies. Still, most patients with late stage disease do not respond, and the side effects can be severe. Stimulation of the CD40 pathway to initiate anti-tumor immunity is a promising alternative. Herein, we demonstrate immune profiling data from melanoma patients treated with an adenovirus-based CD40 ligand gene therapy (AdCD40L).

Methods
Peripheral blood mononuclear cells and plasma were collected from malignant melanoma patients (n = 15) enrolled in a phase I/IIa study investigating intratumoral delivery of AdCD40L with or without low dose cyclophosphamide. Cells were analyzed by flow cytometry while plasma samples were analyzed by a multi-array proteomics.

Results
All patients had an increased Teffector/Tregulatory cell ratio post therapy. Simultaneously, the death receptors TNFR1 and TRAIL-R2 were significantly up-regulated post treatment. Stem cell factor (SCF), E-selectin, and CD6 correlated to enhanced overall survival while a high level of granulocytic myeloid-derived suppressor cells (gMDSCs), IL8, IL10, TGFb1, CCL4, PlGF and Fl3t ligand was highest in patients with short survival.

Conclusions
AdCD40L intratumoral injection induced desirable systemic immune effects that correlated to prolonged survival. Further studies using CD40 stimulation in malignant melanoma are warranted.
Trial registration The 002:CD40L trial “Phase I/IIa AdCD40L Immunogene Therapy for Malignant Melanoma and Other Solid Tumors” (clinicalTrials.gov identifier: NCT01455259) was registered at September 2011
Fixed set of set valued mappings with set valued domain in terms of start set on a metric space with a graph
Authors: Debnath Pradip , Choudhury Binayak S , Neog Murchana 
Published: April 2017

AbstractIn this article, we define the new concept of a fixed set for a set valued map with set valued domain in the setting of metric space endowed with a directed graph. This notion of fixed set is analogous to the notion of a fixed point for a multivalued map and not for a classical single-valued map. We also introduce the new concept of the start set of a graph whose vertices are closed and bounded subsets of a metric space. Characterizations for such a graph to have a start set are given. Further, the notion of a self-path set valued map is defined and its relation with the start set is established. Finally, the existence of fixed sets is established in this context.
Spatiotemporal regulation of cell fusion by JNK and JAK/STAT signaling during Drosophila wound healing
Authors: Lee Ji-Hyun , Lee Chan-wool , Park Si-Hyoung , Choe Kwang-Min 
Published: April 2017

ABSTRACT
Cell–cell fusion is widely observed during development and disease, and imposes a dramatic change on participating cells. Cell fusion should be tightly controlled, but the underlying mechanism is poorly understood. Here, we found that the JAK/STAT pathway suppressed cell fusion during wound healing in the Drosophila larval epidermis, restricting cell fusion to the vicinity of the wound. In the absence of JAK/STAT signaling, a large syncytium containing a 3-fold higher number of nuclei than observed in wild-type tissue formed in wounded epidermis. The JAK/STAT ligand-encoding genes upd2 and upd3 were transcriptionally induced by wounding, and were required for suppressing excess cell fusion. JNK (also known as Basket in flies) was activated in the wound vicinity and activity peaked at ∼8 h after injury, whereas JAK/STAT signaling was activated in an adjoining concentric ring and activity peaked at a later stage. Cell fusion occurred primarily in the wound vicinity, where JAK/STAT activation was suppressed by fusion-inducing JNK signaling. JAK/STAT signaling was both necessary and sufficient for the induction of βPS integrin (also known as Myospheroid) expression, suggesting that the suppression of cell fusion was mediated at least in part by integrin protein.
Multiplex Tandem Mass Spectrometry Enzymatic Activity Assay for Newborn Screening of the Mucopolysaccharidoses and Type 2 Neuronal Ceroid Lipofuscinosis
Authors: Liu Yang , Yi Fan , Kumar Arun Babu , Kumar Chennamaneni Naveen , Hong Xinying , Scott C Ronald , Gelb Michael H , Turecek Frantisek 
Published: April 2017

Abstract

BACKGROUND
We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL).

METHODS
New substrates were prepared and characterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB). These assays were combined with previously developed assays to provide a multiplex LC-MS/MS assay of 7 lysosomal storage diseases. Multiple reaction monitoring of ion dissociations for enzyme products and deuterium-labeled internal standards was used to quantify the enzyme activities.

RESULTS
Deidentified DBS samples from 62 nonaffected newborns were analyzed to simultaneously determine (run time 2 min per DBS) the activities of TPP1, NAGLU, and GUSB, along with those for α-iduronidase (IDUA), iduronate-2-sulfatase (I2S), N-acetylgalactosamine-6-sulfatase (GALNS), and N-acetylgalactosamine-4-sulfatase (ARSB). The activities measured in the 7-plex format showed assay response-to-blank-activity ratios (analytical ranges) of 102–909 that clearly separated healthy infants from affected children.

CONCLUSIONS
The new multiplex assay provides a robust comprehensive newborn screening assay for the mucopolysaccharidoses. The method has been expanded to include additional lysosomal storage diseases.
Jurisprudent Shift in China: A Functional Interpretation
Authors: Qiao Cong-rui 
Published: April 2017

Abstract
In light of the limitation of normative narratives of Chinese jurisprudence, this article proposes a functional interpretation of how the law evolved in China and how the jurisprudence was formalized to cope with major social challenges in the society. Through an examination on historiographical findings and official documentation, the article outlines the extent to which legal norms and institutions succeed in accommodating dominant social consciousness in a particular context, and reveals the interplay between norms and mechanisms respectively in ancient China (around 400 B.C. to the mid-19th century) and modern China (the late 19th century to the 20th century) respectively. The article concludes that in China’s transformational context where people are on a frequent mobility and tend to be assertive toward the government, the jurisprudent shift is likely to be formulated in favor of procedural fairness, insensitive to kinship relations and political beliefs. The shift so too allows one to predict the governant reactions to individual claims.
Research Article. The Prevalence of Dysphotopsia in Patients with Recent Cataract Surgery
Authors: Fişuş Andreea Dana , Madaras Zoltán , Horváth Karin Ursula 
Published: April 2017

Abstract
Pseudophakic dysphotopsia are becoming increasingly important as unwanted side effect after cataract surgery. Objective: The purpose of this study is to compare the photic symptoms experienced by patients after cataract surgery. Material and method: This is a prospective study that included 105 eyes from 99 patients, which underwent uncomplicated phacoemulsification and IOL implantation, between June 2015 and June 2016, performed at Ophthalmology Clinic Tg Mureș. Patients without visually consequential ocular co-morbidity completed a questionnaire, designed to assess subjectively perceived visual functioning and identify symptoms of dysphotopsia. Results: From the total number of patient, hydrophobic lenses were implanted in 95 patients and 10 patients received hydrophilic lenses. Photic effects were reported in 18% of treated eyes. Although the percentage of dysphotopsia is higher in the hydrophobic lenses category, there was no significant statistical difference between the two categories. Conclusion: The incidence and significance should not be overlooked, thus visual acuity is not enough for evaluating postoperative visual function.
Performance characteristics of low global warming potential R134a alternative refrigerants in ejector-expansion refrigeration system
Authors: Mishra Shubham , Sarkar Jahar 
Published: April 2017

AbstractPerformance assessment of ejector-expansion vapor compression refrigeration system with eco-friendly R134a alternative refrigerants (R152a, R1234yf, R600a, R600, R290, R161, R32, and propylene) is presented for air-conditioning application. Ejector has been modeled by considering experimental data based correlations of component efficiencies to take care of all irreversibilities. Ejector area ratio has been optimized based on maximum coefficient of performance (COP) for typical air-conditioner operating temperatures. Selected refrigerants have been compared based on area ratio, pressure lift ratio, entrainment ratio, COP, COP improvement and volumetric cooling capacity. Effects of normal boiling point and critical point on the performances have been studied as well. Using ejector as an expansion device, maximum improvement in COP is noted in R1234yf (10.1%), which reduces the COP deviation with R134a (4.5% less in basic cycle and 2.5% less in ejector cycle). Hence, R1234yf seems to be best alternative for ejector expansion system due to its mild flammability and comparable volumetric capacity and cooling COP. refrigerant R161 is superior to R134a in terms of both COP and volumetric cooling capacity, although may be restricted for low capacity application due to its flammability.
PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks
Authors: Cheng Tian-Yin , Liu Guo-Hua 
Published: April 2017

AbstractTicks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with
Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
Authors: Guzmán-Cornejo Carmen , García-Prieto Luis , Rebollo-Hernández Andrea , Venzal José M. , Nava Santiago , Sánchez-Montes Sokani 
Published: April 2017

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Mass Distribution and Gravitational Potential of the Milky Way
Authors: Ninković Slobodan 
Published: April 2017

Abstract
Models of mass distribution in the Milky Way are discussed where those yielding the potential analytically are preferred. It is noted that there are three main contributors to the Milky Way potential: bulge, disc and dark halo. In the case of the disc the Miyamoto-Nagai formula, as simple enough, has shown as a very good solution, but it has not been able to satisfy all requirements. Therefore, improvements, such as adding new terms or combining several Miyamoto-Nagai terms, have been attempted. Unlike the disc, in studying the bulge and dark halo the flattening is usually neglected, which offers the possibility of obtaining an exact solution of the Poisson equation. It is emphasized that the Hernquist formula, used very often for the bulge potential, is a special case of another formula and the properties of that formula are analysed. In the case of the dark halo, the slopes of its cumulative mass for the inner and outer parts are explained through a new formalism presented here for the first time.