bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of a highly potent glucocorticoid for asthma treatment
Authors: He Yuanzheng , Shi Jingjing , Yi Wei , Ren Xin , Gao Xiang , Li Jianshuang , Wu Nanyan , Weaver Kevin , Xie Qian , Khoo Sok Kean , Yang Tao , Huang Xiaozhu , Melcher Karsten , Xu H Eric 
Published: December 2015

AbstractGlucocorticoids are the most effective treatment for asthma. However, their clinical applications are limited by low efficacy in severe asthma and by undesired side effects associated with high dose or prolonged use. The most successful approach to overcome these limitations has been the development of highly potent glucocorticoids that can be delivered to the lungs by inhalation to achieve local efficacy with minimal systemic effects. On the basis of our previous structural studies, we designed and developed a highly potent glucocorticoid, VSGC12, which showed an improved anti-inflammation activity in both cell-based reporter assays and cytokine inhibition experiments, as well as in a gene expression profiling of mouse macrophage RAW264.7 cells. In a mouse asthma model, VSGC12 delivered a higher efficacy than fluticasone furoate, a leading clinical compound, in many categories including histology and the number of differentiated immune cells. VSGC12 also showed a higher potency than fluticasone furoate in repressing most asthma symptoms. Finally, VSGC12 showed a better side effect profile than fluticasone furoate at their respective effective doses, including better insulin response and less bone loss in an animal model. The excellent therapeutic and side effect properties of VSGC12 provide a promising perspective for developing this potent glucocorticoid as a new effective drug for asthma.
Sepsis induces long-term metabolic and mitochondrial muscle stem cell dysfunction amenable by mesenchymal stem cell therapy
Authors: Rocheteau P. , Chatre L. , Briand D. , Mebarki M. , Jouvion G. , Bardon J. , Crochemore C. , Serrani P. , Lecci P. P. , Latil M. , Matot B. , Carlier P. G. , Latronico N. , Huchet C. , Lafoux A. , Sharshar T. , Ricchetti M. , Chrétien F. 
Published: December 2015

AbstractSepsis, or systemic inflammatory response syndrome, is the major cause of critical illness resulting in admission to intensive care units. Sepsis is caused by severe infection and is associated with mortality in 60% of cases. Morbidity due to sepsis is complicated by neuromyopathy, and patients face long-term disability due to muscle weakness, energetic dysfunction, proteolysis and muscle wasting. These processes are triggered by pro-inflammatory cytokines and metabolic imbalances and are aggravated by malnutrition and drugs. Skeletal muscle regeneration depends on stem (satellite) cells. Herein we show that mitochondrial and metabolic alterations underlie the sepsis-induced long-term impairment of satellite cells and lead to inefficient muscle regeneration. Engrafting mesenchymal stem cells improves the septic status by decreasing cytokine levels, restoring mitochondrial and metabolic function in satellite cells, and improving muscle strength. These findings indicate that sepsis affects quiescent muscle stem cells and that mesenchymal stem cells might act as a preventive therapeutic approach for sepsis-related morbidity.
Natural cytotoxicity receptor splice variants orchestrate the distinct functions of human natural killer cell subtypes
Authors: Siewiera Johan , Gouilly Jordi , Hocine Hocine-Rachid , Cartron Géraldine , Levy Claude , Al-Daccak Reem , Jabrane-Ferrat Nabila 
Published: December 2015

AbstractThe natural cytotoxicity receptors NKp46/NCR1, NKp44/NCR2 and NKp30/NCR3 are critical for natural killer (NK) cell functions. Their genes are transcribed into several splice variants whose physiological relevance is not yet fully understood. Here we report that decidua basalis NK (dNK) cells of the pregnant uterine mucosa and peripheral blood NK (pNK) cells, two functionally distinct subsets of the physiological NK cell pool, display differential expression of NKp30/NCR3 and NKp44/NCR2 splice variants. The presence of cytokines that are enriched within the decidual microenvironment is sufficient to convert the splice variant profile of pNK cells into one similar to that of dNK cells. This switch is associated with decreased cytotoxic function and major adaptations to the secretome, hallmarks of the decidual phenotype. Thus, NKp30/NCR3 and NKp44/NCR2 splice variants delineate functionally distinct NK cell subsets. To our knowledge, this is the first conclusive evidence underlining the physiological importance of NCR splice variants.
Breaking of the site-bond percolation universality in networks
Authors: Radicchi Filippo , Castellano Claudio 
Published: December 2015

AbstractThe stochastic addition of either vertices or connections in a network leads to the observation of the percolation transition, a structural change with the appearance of a connected component encompassing a finite fraction of the system. Percolation has always been regarded as a substrate-dependent but model-independent process, in the sense that the critical exponents of the transition are determined by the geometry of the system, but they are identical for the bond and site percolation models. Here, we report a violation of such assumption. We provide analytical and numerical evidence of a difference in the values of the critical exponents between the bond and site percolation models in networks with null percolation thresholds, such as scale-free graphs with diverging second moment of the degree distribution. We discuss possible implications of our results in real networks, and provide additional insights on the anomalous nature of the percolation transition with null threshold.
Novel Compound Heterozygous CBS Mutations Cause Homocystinuria in a Han Chinese Family
Authors: Gong Bo , Liu Liping , Li Zhiwei , Ye Zimeng , Xiao Ying , Zeng Guangqun , Shi Yi , Wang Yumeng , Feng Xiaoyun , Li Xiulan , Hao Fang , Liu Xiaoqi , Qu Chao , Li Yuanfeng , Mu Guoying , Yang Zhenglin 
Published: December 2015

AbstractThe cystathionine β-synthase (CBS) gene has been shown to be related to homocystinuria. This study was aimed to detect the mutations in CBS in a Han Chinese family with homocystinuria. A four-generation family from Shandong Province of China was recruited in this study. All available members of the family underwent comprehensive medical examinations. Genomic DNA was collected from peripheral blood of all the participants. The coding sequence of CBS was amplified by polymerase chain reaction (PCR), followed by direct DNA sequencing. Among all the family members, three affected individuals showed typical clinical features of homocystinuria. Two novel compound heterozygous mutations in the CBS gene, c.407T > C (p. L136P) and c.473C > T (p.A158V), were identified by sequencing analysis in this family. Both of the two missense mutations were detected in the three patients. Other available normal individuals, including the patients’ parents, grand parents, her younger sister and brother in this family either carried one of the two mutations, or none. In addition, the two mutations were not found in 600 ethnically matched normal controls. This study provides a mutation spectrum of CBS resulting in homocystinuriain a Chinese population, which may shed light on the molecular pathogenesis and clinical diagnosis of CBS-associated homocystinuria.
Coherent controlization using superconducting qubits
Authors: Friis Nicolai , Melnikov Alexey A. , Kirchmair Gerhard , Briegel Hans J. 
Published: December 2015

AbstractCoherent controlization, i.e., coherent conditioning of arbitrary single- or multi-qubit operations on the state of one or more control qubits, is an important ingredient for the flexible implementation of many algorithms in quantum computation. This is of particular significance when certain subroutines are changing over time or when they are frequently modified, such as in decision-making algorithms for learning agents. We propose a scheme to realize coherent controlization for any number of superconducting qubits coupled to a microwave resonator. For two and three qubits, we present an explicit construction that is of high relevance for quantum learning agents. We demonstrate the feasibility of our proposal, taking into account loss, dephasing and the cavity self-Kerr effect.
A combined system of microbial fuel cell and intermittently aerated biological filter for energy self-sufficient wastewater treatment
Authors: Dong Yue , Feng Yujie , Qu Youpeng , Du Yue , Zhou Xiangtong , Liu Jia 
Published: December 2015

AbstractEnergy self-sufficiency is a highly desirable goal of sustainable wastewater treatment. Herein, a combined system of a microbial fuel cell and an intermittently aerated biological filter (MFC-IABF) was designed and operated in an energy self-sufficient manner. The system was fed with synthetic wastewater (COD = 1000 mg L−1) in continuous mode for more than 3 months at room temperature (~25 °C). Voltage output was increased to 5 ± 0.4 V using a capacitor-based circuit. The MFC produced electricity to power the pumping and aeration systems in IABF, concomitantly removing COD. The IABF operating under an intermittent aeration mode (aeration rate 1000 ± 80 mL h−1) removed the residual nutrients and improved the water quality at HRT = 7.2 h. This two-stage combined system obtained 93.9% SCOD removal and 91.7% TCOD removal (effluent SCOD = 61 mg L−1, TCOD = 82.8 mg L−1). Energy analysis indicated that the MFC unit produced sufficient energy (0.27 kWh m−3) to support the pumping system (0.014 kWh m−3) and aeration system (0.22 kWh m−3). These results demonstrated that the combined MFC-IABF system could be operated in an energy self-sufficient manner, resulting to high-quality effluent.
Mean platelet volume in young children with urinary tract infection
Authors: Lee I Re , Shin Jae Il , Park Se Jin , Oh Ji Young , Kim Ji Hong 
Published: December 2015

AbstractMean platelet volume (MPV) has not yet been well-established in urinary tract infection (UTI). The purpose of this study was to evaluate the role of MPV as an acute phase reactant in children with UTI. Data from 118 young children (<2 years) with UTI between 2012 and 2013 were grouped as acute pyelonephritis (APN) and lower UTI according to the dimercaptosuccinic acid (DMSA) scan abnormalities. MPV, platelet distribution width (PDW) platelet count and other infection markers (white blood cell [WBC] count, erythrocyte sedimentation rate [ESR] and C-reactive protein [CRP]) were measured. WBC (P = 0.001), ESR (P = 0.005), CRP (P < 0.001) and MPV levels (P = 0.011) were significantly higher in the APN group than those in the lower UTI group. MPV positively correlated with PDW, CRP and negatively with platelet count. Multiple logistic regression analyses showed that CRP and MPV were independent predictive factors for APN patients. However, the area under the Receiver Operating Characteristic (ROC) curve analysis for MPV was lower than CRP. Our results suggest that MPV can be an inflammatory marker in UTI, but the predictive value of MPV was not superior to CRP in the diagnosis of APN.
Degassing during quiescence as a trigger of magma ascent and volcanic eruptions
Authors: Girona Társilo , Costa Fidel , Schubert Gerald 
Published: December 2015

AbstractUnderstanding the mechanisms that control the start-up of volcanic unrest is crucial to improve the forecasting of eruptions at active volcanoes. Among the most active volcanoes in the world are the so-called persistently degassing ones (e.g., Etna, Italy; Merapi, Indonesia), which emit massive amounts of gas during quiescence (several kilotonnes per day) and erupt every few months or years. The hyperactivity of these volcanoes results from frequent pressurizations of the shallow magma plumbing system, which in most cases are thought to occur by the ascent of magma from deep to shallow reservoirs. However, the driving force that causes magma ascent from depth remains unknown. Here we demonstrate that magma ascent can be triggered by the passive release of gas during quiescence, which induces the opening of pathways connecting deep and shallow magma reservoirs. This top-down mechanism for volcanic eruptions contrasts with the more common bottom-up mechanisms in which magma ascent is only driven by processes occurring at depth. A cause-effect relationship between passive degassing and magma ascent can explain the fact that repose times are typically much longer than unrest times preceding eruptions and may account for the so frequent unrest episodes of persistently degassing volcanoes.
Comparative study of diversity based on heat tolerant-related morpho-physiological traits and molecular markers in tall fescue accessions
Authors: Sun Xiaoyan , Xie Yan , Bi Yufang , Liu Jianping , Amombo Erick , Hu Tao , Fu Jinmin 
Published: December 2015

AbstractHeat stress is a critical challenge to tall fescue (Festuca arundinaceaSchreb.) in many areas of the globe and variations in genetic structure and functional traits is for the efficient breeding programs on developing heat tolerant cultivars. Tolerant-related morpho-physiological traits and simple sequence repeat (SSR) markers were employed to survey genetic diversity in greenhouse and growth chamber trials. 100 tall fescue accessions, including 8 commercial cultivars and 92 natural genotypes, showed a high variation in phenotypic performance under heat stress. Based on standardized heat tolerant-related morpho-physiological data, all tall fescue accessions were clustered into five groups. The accessions with similar heat tolerance were likely to be clustered in the same group. The highest genetic diversity was obtained for accessions from Africa judged by Nei’s gene diversity (0.2640) and PIC (0.2112). All grass accessions could be divided into three major groups based on SSR markers, which was partially congruous to the geographical regions and history of introduction. A low correlation was found between morpho-physiological traits and SSR markers by Mantel test. The patterns in morpho-physiological trait variations and genetic diversity associated with heat tolerance were useful to design breeding programs for developing heat stress resistance in tall fescue.
Graphene flakes under controlled biaxial deformation
Authors: Androulidakis Charalampos , Koukaras Emmanuel N. , Parthenios John , Kalosakas George , Papagelis Konstantinos , Galiotis Costas 
Published: December 2015

AbstractThin membranes, such as monolayer graphene of monoatomic thickness, are bound to exhibit lateral buckling under uniaxial tensile loading that impairs its mechanical behaviour. In this work, we have developed an experimental device to subject 2D materials to controlled equibiaxial strain on supported beams that can be flexed up or down to subject the material to either compression or tension, respectively. Using strain gauges in tandem with Raman spectroscopy measurements, we monitor the G and 2D phonon properties of graphene under biaxial strain and thus extract important information about the uptake of stress under these conditions. The experimental shift over strain for the G and 2D Raman peaks were found to be in the range of 62.3 ± 5 cm–1/% and 148.2 ± 6 cm–1/%, respectively, for monolayer but also bilayer graphenes. The corresponding Grüneisen parameters for the G and 2D peaks were found to be between 1.97 ± 0.15 and 2.86 ± 0.12, respectively. These values agree reasonably well with those obtained from small-strain bubble-type experiments. The results presented are also backed up by classical and ab initio molecular dynamics simulations and excellent agreement of Γ-E2g shifts with strains and the Grüneisen parameter was observed.
Physical activity in adulthood: genes and mortality
Authors: Karvinen Sira , Waller Katja , Silvennoinen Mika , Koch Lauren G. , Britton Steven L. , Kaprio Jaakko , Kainulainen Heikki , Kujala Urho M. 
Published: December 2015

AbstractObservational studies report a strong inverse relationship between leisure-time physical activity and all-cause mortality. Despite suggestive evidence from population-based associations, scientists have not been able to show a beneficial effect of physical activity on the risk of death in controlled intervention studies among individuals who have been healthy at baseline. On the other hand, high cardiorespiratory fitness is known to be a strong predictor of reduced mortality, even more robust than physical activity level itself. Here, in both animals and/or human twins, we show that the same genetic factors influence physical activity levels, cardiorespiratory fitness and risk of death. Previous observational follow-up studies in humans suggest that increasing fitness through physical activity levels could prolong life; however, our controlled interventional study with laboratory rats bred for low and high intrinsic fitness contrast with these findings. Also, we find no evidence for the suggested association using pairwise analysis among monozygotic twin pairs who are discordant in their physical activity levels. Based on both our animal and human findings, we propose that genetic pleiotropy might partly explain the frequently observed associations between high baseline physical activity and later reduced mortality in humans.
DeFiNe: an optimisation-based method for robust disentangling of filamentous networks
Authors: Breuer David , Nikoloski Zoran 
Published: December 2015

AbstractThread-like structures are pervasive across scales, from polymeric proteins to root systems to galaxy filaments and their characteristics can be readily investigated in the network formalism. Yet, network links usually represent only parts of filaments, which, when neglected, may lead to erroneous conclusions from network-based analyses. The existing alternatives to detect filaments in network representations require tuning of parameters over a large range of values and treat all filaments equally, thus, precluding automated analysis of diverse filamentous systems. Here, we propose a fully automated and robust optimisation-based approach to detect filaments of consistent intensities and angles in a given network. We test and demonstrate the accuracy of our solution with contrived, biological and cosmic filamentous structures. In particular, we show that the proposed approach provides powerful automated means to study properties of individual actin filaments in their network context. Our solution is made publicly available as an open-source tool, “DeFiNe”, facilitating decomposition of any given network into individual filaments.
Discrimination of two-qubit unitaries via local operations and classical communication
Authors: Bae Joonwoo 
Published: December 2015

AbstractDistinguishability is a fundamental and operational measure generally connected to information applications. In quantum information theory, from the postulates of quantum mechanics it often has an intrinsic limitation, which then dictates and also characterises capabilities of related information tasks. In this work, we consider discrimination between bipartite two-qubit unitary transformations by local operations and classical communication (LOCC) and its relations to entangling capabilities of given unitaries. We show that a pair of entangling unitaries which do not contain local parts, if they are perfectly distinguishable by global operations, can also be perfectly distinguishable by LOCC. There also exist non-entangling unitaries, e.g. local unitaries, that are perfectly discriminated by global operations but not by LOCC. The results show that capabilities of LOCC are strictly restricted than global operations in distinguishing bipartite unitaries for a finite number of repetitions, contrast to discrimination of a pair of bipartite states and also to asymptotic discrimination of unitaries.
Rapid loss of antipredatory behaviour in captive-bred birds is linked to current avian invasions
Authors: Carrete Martina , Tella José L. 
Published: December 2015

AbstractDespite the importance of behaviour in conservation biology, there have been few studies that address behaviour in areas such as invasion ecology. There is an urgent need to identify specific traits that facilitate the establishment and spread of alien species to prevent biological invasions and their impact on biodiversity. Changes in antipredatory behaviour in captivity have been proposed to explain the higher invasiveness of wild-caught exotic species. We experimentally tested this hypothesis by assessing the response of wild-caught and captive-bred cage birds facing an approaching predator and their ability to escape from human capture, using species available in the Spanish pet market. Results showed the loss of antipredatory responses and escape abilities in captive-bred birds compared with wild-caught ones. An intraspecific comparison between wild-caught and the first generation of captive-bred birds pointed to a rapid behavioural loss in captivity (individual lifetime) rather than to differences among species (evolutionary exposure). In the context of current avian invasions, the proportion of individuals showing antipredatory responses within a species was positively related to the likelihood of the species being found escaped and breeding in the wild. These results offer a link between behaviour, fitness and the invasion syndrome in birds.
A YAP/TAZ-miR-130/301 molecular circuit exerts systems-level control of fibrosis in a network of human diseases and physiologic conditions
Authors: Bertero Thomas , Cottrill Katherine A. , Annis Sofia , Bhat Balkrishen , Gochuico Bernadette R. , Osorio Juan C. , Rosas Ivan , Haley Kathleen J. , Corey Kathleen E. , Chung Raymond T. , Nelson Chau B. , Chan Stephen Y. 
Published: December 2015

AbstractThe molecular origins of fibrosis affecting multiple tissue beds remain incompletely defined. Previously, we delineated the critical role of the control of extracellular matrix (ECM) stiffening by the mechanosensitive microRNA-130/301 family, as activated by the YAP/TAZ co-transcription factors, in promoting pulmonary hypertension (PH). We hypothesized that similar mechanisms may dictate fibrosis in other tissue beds beyond the pulmonary vasculature. Employing an in silico combination of microRNA target prediction, transcriptomic analysis of 137 human diseases and physiologic states and advanced gene network modeling, we predicted the microRNA-130/301 family as a master regulator of fibrotic pathways across a cohort of seemingly disparate diseases and conditions. In two such diseases (pulmonary fibrosis and liver fibrosis), inhibition of microRNA-130/301 prevented the induction of ECM modification, YAP/TAZ and downstream tissue fibrosis. Thus, mechanical forces act through a central feedback circuit between microRNA-130/301 and YAP/TAZ to sustain a common fibrotic phenotype across a network of human physiologic and pathophysiologic states. Such re-conceptualization of interconnections based on shared systems of disease and non-disease gene networks may have broad implications for future convergent diagnostic and therapeutic strategies.