bluuebunny/crossref_metadata_2025_split huggingface dataset:
Germination and persistence in soil of the dimorphic diaspores of Atriplex centralasiatica
Authors: Zhang Rui , Wang Yan Rong , Baskin Jerry M. , Baskin Carol C. , Luo Kai , Hu Xiao Wen 
Published: August 2016

AbstractThe fruit plus accessory parts, and thus not only the fruit or seed, is the diaspore in some species of flowering plants. Atriplex centralasiatica, a summer annual salt-secreting cold-desert halophyte of central Asia that produces flat and humped diaspores, is such a species. The dispersal/germination units of this diaspore-heteromorphic species are fruits enclosed in persistent ‘bracteoles’. Germination of black and brown fruits (i.e. ‘bracteoles’ removed) of this species has been studied in some detail previously, but little attention has been given to the germination biology of the intact diaspores. The aim of this study was to compare the germination biology of the intact diaspores of A. centralasiatica, and their fates in the field during 12 months on the soil surface and buried at 5-cm depth. Fresh flat diaspores germinated to 42.7–51.3% in light (12-h photoperiod) and to 16.0–59.3% in constant dark, over a temperature range of 15–20/30°C, while no freshly matured humped diaspores did so under any temperature/light regime. Neither gibberellin (GA3) nor potassium nitrate (KNO3) had an effect on germination of either diaspore. Both diaspores readily imbibed water, and removal of ‘bracteoles’ released most of the dormancy. Thus, the ‘bracteoles’ are primarily responsible for diaspore dormancy in A. centralasiatica. Humped diaspores persisted for a longer period in the soil than flat ones. The germination requirements of intact natural dispersal/germination units of A. centralasiatica differ from those previously reported for fruits of this species.
Suburban growth in Adelaide, South Australia, 1850–1930: speculation and economic opportunity
Authors: MCGREEVY MICHAEL 
Published: August 2016

ABSTRACTSuburbs are significant to any understanding of Australian urbanization as they have been the dominant organizational element in the morphology of metropolitan areas. A case-study of suburban growth in Adelaide, South Australia, in the period from 1850 to 1930 suggests that dominant accounts of Australian suburbs of the era, as places of tranquillity, leisure, home and family, whose growth was driven by aspiration and social mobility, are largely illusory. Suburban growth was instead driven by speculation and economic opportunity. Accounts of commercial, recreational and industrial activity in Adelaide's suburban municipalities of the time suggests economically and socially diverse communities. Whereas the desire for the quarter or half acre block in the suburbs was most often due to its productive potential rather than bourgeois aspirations for seclusion and semi-rural tranquillity.
Phantoms of modernity: the 1894 anarchist furor in the making of modern Guatemala City
Authors: KIRKPATRICK MICHAEL D. 
Published: August 2016

ABSTRACTFollowing a spate of anarchist bombings and assassinations in Europe, thegente decenteof Guatemala City began to describe local events using the language of anarchism. The 1894 anarchist furor spoke to two tendencies that had shaped Guatemala City since the 1870s. The first was the cosmopolitan desire of thegente decente. Facilitated by cosmopolitan bridge figures, trends and fashions from Europe and especially Paris shaped the cultural lexicon of Guatemala City's elite. Secondly, the anarchist furor reflected the misgivings of thegente decentetoward urban disorder and malcontents as they conflated anarchism and anarchy.
MOZART, KIRNBERGER AND THE IDEA OF MUSICAL PURITY: REVISITING TWO SKETCHES FROM 1782
Authors: RATHEY MARKUS 
Published: August 2016

ABSTRACTWhen Beethoven praised Mozart'sDie Zauberflöte, he emphasized the multitude of musical styles and genres to be found in the opera, ranging from folk tunes to arias to hymn-like textures. The most extraordinary stylistic and generic allusions occur during the ‘Song of the Armoured Men’ in Act 2. This movement owes its extraordinary character to a ‘baroque’ accompaniment and a Lutheran-hymn quotation, the source and meaning of which continue to be discussed in Mozart research. While scholars have often suggested that Mozart took the hymn melody from Johann Philipp Kirnberger'sDie Kunst des reinen Satzes in der Musik(where it is quoted without its text), Reinhold Hammerstein argues that because the composer also appears to have known the hymn's text, he must have encountered the melody elsewhere. This article, based on a study of Mozart's sketches forDie Zauberflöteand a close reading of Kirnberger's writings, supports the thesis that Mozart borrowed the hymn melody – and significant details of his setting of it in the ‘Song of the Armoured Men’ – fromDie Kunst des reinen Satzes in der Musik.
ON THE VENUES FOR AND DECLINE OF THEACCADEMIESAT ESZTERHÁZA IN HAYDN'S TIME
Authors: MALINA JÁNOS 
Published: August 2016

ABSTRACTThis article examines various eighteenth-century sources to determine whether they confirm the present practice of calling a first-floor hall of the Fertőd (Eszterháza) palace the ‘music room’. While the answer is essentially negative, we learn that the neighbouring ceremonial hall was used by Empress Maria Theresia for a banquet with some music-making in 1773, and that two more spaces on the ground floor served regularly as the ‘summer music halls’. So where did the ‘real’, quality concerts take place? A whole body of documentary evidence clearly shows that theaccademiestook place in the opera house orGrosses Theater. Much of this evidence refers to the first opera house, which burnt down in 1779. The practice apparently continued in the new, bigger 1781 opera house, but by then the number of concerts would have been reduced substantially, owing to the Prince's growing addiction to opera. A survey of Haydn's last symphonies and concertos composed for domestic use confirms that regular concerts could not have taken place later than 1783 or, possibly, 1784. However, a long-neglected remark in a contemporary witness report provides direct proof of the inclusion of symphonies in the course of opera performances.
Multiparametric imaging with heterogeneous radiofrequency fields
Authors: Cloos Martijn A. , Knoll Florian , Zhao Tiejun , Block Kai T. , Bruno Mary , Wiggins Graham C. , Sodickson Daniel K. 
Published: August 2016

AbstractMagnetic resonance imaging (MRI) has become an unrivalled medical diagnostic technique able to map tissue anatomy and physiology non-invasively. MRI measurements are meticulously engineered to control experimental conditions across the sample. However, residual radiofrequency (RF) field inhomogeneities are often unavoidable, leading to artefacts that degrade the diagnostic and scientific value of the images. Here we show that, paradoxically, these artefacts can be eliminated by deliberately interweaving freely varying heterogeneous RF fields into a magnetic resonance fingerprinting data-acquisition process. Observations made based on simulations are experimentally confirmed at 7 Tesla (T), and the clinical implications of this new paradigm are illustrated with in vivo measurements near an orthopaedic implant at 3T. These results show that it is possible to perform quantitative multiparametric imaging with heterogeneous RF fields, and to liberate MRI from the traditional struggle for control over the RF field uniformity.
Impacts of neonicotinoid use on long-term population changes in wild bees in England
Authors: Woodcock Ben A. , Isaac Nicholas J. B. , Bullock James M. , Roy David B. , Garthwaite David G. , Crowe Andrew , Pywell Richard F. 
Published: August 2016

AbstractWild bee declines have been ascribed in part to neonicotinoid insecticides. While short-term laboratory studies on commercially bred species (principally honeybees and bumblebees) have identified sub-lethal effects, there is no strong evidence linking these insecticides to losses of the majority of wild bee species. We relate 18 years of UK national wild bee distribution data for 62 species to amounts of neonicotinoid use in oilseed rape. Using a multi-species dynamic Bayesian occupancy analysis, we find evidence of increased population extinction rates in response to neonicotinoid seed treatment use on oilseed rape. Species foraging on oilseed rape benefit from the cover of this crop, but were on average three times more negatively affected by exposure to neonicotinoids than non-crop foragers. Our results suggest that sub-lethal effects of neonicotinoids could scale up to cause losses of bee biodiversity. Restrictions on neonicotinoid use may reduce population declines.
Left extrastriate body area is sensitive to the meaning of symbolic gesture: evidence from fMRI repetition suppression
Authors: Kubiak Agnieszka , Króliczak Gregory 
Published: August 2016

AbstractFunctional magnetic resonance imaging (fMRI) adaptation (a.k.a. repetition suppression) paradigm was used to test if semantic information contained in object-related (transitive) pantomimes and communicative (intransitive) gestures is represented differently in the occipito-temporal cortex. Participants watched 2.75 s back-to-back videos where the meaning of gesture was either repeated or changed. The just observed (typically second) gesture was then imitated. To maintain participants’ attention, some trials contained a single video. fMRI adaptation –signal decreases– for watching both movement categories were observed particularly in the lateral occipital cortex, including the extrastriate body area (EBA). Yet, intransitive (vs. transitive) gesture specific repetition suppression was found mainly in the left rostral EBA and caudal middle temporal gyrus- the rEBA/cMTG complex. Repetition enhancement (signal increase) was revealed in the precuneus. While the whole brain and region-of-interest analyses indicate that the precuneus is involved only in visuospatial action processing for later imitation, the common EBA repetition suppression discloses sensitivity to the meaning of symbolic gesture, namely the “semantic what” of actions. Moreover, the rEBA/cMTG suppression reveals greater selectivity for conventionalized communicative gesture. Thus, fMRI adaptation shows higher-order functions of EBA, its role in the semantic network and indicates that its functional repertoire is wider than previously thought.
A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index
Authors: Zhang Aiying , Skog Sven , Wang Shengqi , Ke Yang , Zhang Yonghong , Li Kang , He Ellen , Li Ning 
Published: August 2016

AbstractThe Lens culinaris agglutinin-reactive fraction of AFP (AFP-L3) is widely used to screen for hepatocellular carcinoma (HCC) in Japan and China. We developed a chemiluminescent protein microarray for determining the AFP-L3/AFP index (the ratio of AFP-L3 to total AFP, AFP-L3%) by fixing AFP-specific antibodies and Lens culinaris lectin on aldehyde-coated glass slides. Serum samples were tested for AFP using an enzyme-linked immunosorbent assay (ELISA) to validate the microarray. AFP-L3 was detected using Hotgen Biotech glycosyl capture spin column pretreatment technology and ELISA. When the AFP cut-off value was set to 20 ng/ml, the protein microarray displayed 89.74% sensitivity and 100% specificity for HCC diagnosis, and the ELISA displayed 87.17% sensitivity and 100% specificity. When the AFP-L3% cut-off value was set to 0.1, the protein microarray displayed 56.41% sensitivity and 100% specificity for HCC diagnosis, and the ELISA displayed 53.84% sensitivity and 100% specificity. The ROC curve for the HCC diagnosis showed that the AFP area under the ROC curve (AUC = 0.996; 95% CI: 0.986–1.005) was much higher than that of AFP-L3 (AUC = 0.857; 95% CI: 0.769–0.94) and AFP-L3% (AUC = 0.827; CI: 0.730–0.924). The microarray assay used in this study is a highly sensitive, accurate, and efficient assay for the determination of the AFP-L3%.
Ribonuclease 5 facilitates corneal endothelial wound healing via activation of PI3-kinase/Akt pathway
Authors: Kim Kyoung Woo , Park Soo Hyun , Lee Soo Jin , Kim Jae Chan 
Published: August 2016

AbstractTo maintain corneal transparency, corneal endothelial cells (CECs) exert a pump function against aqueous inflow. However, human CECs are arrested in the G1-phase and non-proliferative in vivo. Thus, treatment of corneal endothelial decompensation is limited to corneal transplantation, and grafts are vulnerable to immune rejection. Here, we show that ribonuclease (RNase) 5 is more highly expressed in normal human CECs compared to decompensated tissues. Furthermore, RNase 5 up-regulated survival of CECs and accelerated corneal endothelial wound healing in an in vitro wound of human CECs and an in vivo cryo-damaged rabbit model. RNase 5 treatment rapidly induced accumulation of cytoplasmic RNase 5 into the nucleus, and activated PI3-kinase/Akt pathway in human CECs. Moreover, inhibition of nuclear translocation of RNase 5 using neomycin reversed RNase 5-induced Akt activation. As a potential strategy for proliferation enhancement, RNase 5 increased the population of 5-bromo-2′-deoxyuridine (BrdU)-incorporated proliferating CECs with concomitant PI3-kinase/Akt activation, especially in CECs deprived of contact-inhibition. Specifically, RNase 5 suppressed p27 and up-regulated cyclin D1, D3, and E by activating PI3-kinase/Akt in CECs to initiate cell cycle progression. Together, our data indicate that RNase 5 facilitates corneal endothelial wound healing, and identify RNase 5 as a novel target for therapeutic exploitation.
Insulin receptor alternative splicing is regulated by insulin signaling and modulates beta cell survival
Authors: Malakar Pushkar , Chartarifsky Lital , Hija Ayat , Leibowitz Gil , Glaser Benjamin , Dor Yuval , Karni Rotem 
Published: August 2016

AbstractType 2 Diabetes (T2DM) affects more than 300 million people worldwide. One of the hallmarks of T2DM is peripheral insulin resistance, in part due to unproductive insulin signaling through the insulin receptor. The insulin receptor (INSR) exists as two isoforms, INSR-A and INSR-B, which results from skipping or inclusion of exon 11 respectively. What determines the relative abundance of the different insulin receptor splice variants is unknown. Moreover, it is not yet clear what the physiological roles of each of the isoforms are in normal and diseased beta cells. In this study, we show that insulin induces INSR exon 11 inclusion in pancreatic beta cells in both human and mouse. This occurs through activation of the Ras-MAPK/ERK signaling pathway and up-regulation of the splicing factor SRSF1. Induction of exon 11 skipping by a splice-site competitive antisense oligonucleotide inhibited the MAPK-ERK signaling pathway downstream of the insulin receptor, sensitizing the pancreatic β-cell line MIN6 to stress-induced apoptosis and lipotoxicity. These results assign to insulin a regulatory role in INSR alternative splicing through the Ras-MAPK/ERK signaling pathway. We suggest that in beta cells, INSR-B has a protective role, while INSR-A expression sensitizes beta cells to programmed cell death.
iFish: predicting the pathogenicity of human nonsynonymous variants using gene-specific/family-specific attributes and classifiers
Authors: Wang Meng , Wei Liping 
Published: August 2016

AbstractAccurate prediction of the pathogenicity of genomic variants, especially nonsynonymous single nucleotide variants (nsSNVs), is essential in biomedical research and clinical genetics. Most current prediction methods build a generic classifier for all genes. However, different genes and gene families have different features. We investigated whether gene-specific and family-specific customized classifiers could improve prediction accuracy. Customized gene-specific and family-specific attributes were selected with AIC, BIC, and LASSO, and Support Vector Machine classifiers were generated for 254 genes and 152 gene families, covering a total of 5,985 genes. Our results showed that the customized attributes reflected key features of the genes and gene families, and the customized classifiers achieved higher prediction accuracy than the generic classifier. The customized classifiers and the generic classifier for other genes and families were integrated into a new tool named iFish (integrated Functional inference of SNVs in human, http://ifish.cbi.pku.edu.cn). iFish outperformed other methods on benchmark datasets as well as on prioritization of candidate causal variants from whole exome sequencing. iFish provides a user-friendly web-based interface and supports other functionalities such as integration of genetic evidence. iFish would facilitate high-throughput evaluation and prioritization of nsSNVs in human genetics research.
Genome-wide DNA methylation analysis in obsessive-compulsive disorder patients
Authors: Yue Weihua , Cheng Weiqiu , Liu Zhaorui , Tang Yi , Lu Tianlan , Zhang Dai , Tang Muni , Huang Yueqin 
Published: August 2016

AbstractLiteratures have suggested that not only genetic but also environmental factors, interactively accounted for susceptibility of obsessive-compulsive disorder (OCD). DNA methylation may regulate expression of genes as the heritable epigenetic modification. The examination for genome-wide DNA methylation was performed on blood samples from 65 patients with OCD, as well as 96 healthy control subjects. The DNA methylation was examined at over 485,000 CpG sites using the Illumina Infinium Human Methylation450 BeadChip. As a result, 8,417 probes corresponding to 2,190 unique genes were found to be differentially methylated between OCD and healthy control subjects. Of those genes, 4,013 loci were located in CpG islands and 2,478 were in promoter regions. These includedBCYRN1, BCOR, FGF13, HLA-DRB1, ARX, etc., which have previously been reported to be associated with OCD. Pathway analyses indicated that regulation of actin cytoskeleton, cell adhesion molecules (CAMs), actin binding, transcription regulator activity and other pathways might be further associated with risk of OCD. Unsupervised clustering analysis of the top 3,000 most variable probes revealed two distinct groups with significantly more people with OCD in cluster one compared with controls (67.74% of casesv.s.27.13% of controls, Chi-square = 26.011,df = 1,P = 3.41E-07). These results strongly suggested that differential DNA methylation might play an important role in etiology of OCD.
Out-of-Plane Coordinated Porphyrin Nanotubes with Enhanced Singlet Oxygen Generation Efficiency
Authors: Zhao Qiang , Wang Yao , Xu Yanshuang , Yan Yun , Huang Jianbin 
Published: August 2016

AbstractA supramolecular porphyrin nanotube displaying J-aggregation feature was constructed by out-of-plane coordinated bismuth-porphyrin. Significantly, compared to traditional J-aggregated porphyrin suffering from fluorescence and singlet oxygen quenching, the nanotube exhibits excellent bio-imaging ability and enhanced production efficiency of singlet oxygen. The out-of-plane structure of bismuth to porphyrin makes the aggregation an appropriate material for theranostics. Furthermore, it is also a potential radio-therapeutic drug owing to the presence of radio-active bismuth. Thus, the self-assembly of out-of-plane coordinated porphyrin can be a facile approach toward effective therapy of tumors and other diseases.
Impacts of Conformational Geometries in Fluorinated Alkanes
Authors: Brandenburg Tim , Golnak Ronny , Nagasaka Masanari , Atak Kaan , Sreekantan Nair Lalithambika Sreeju , Kosugi Nobuhiro , Aziz Emad F. 
Published: August 2016

AbstractResearch of blood substitute formulations and their base materials is of high scientific interest. Especially fluorinated microemulsions based on perfluorocarbons, with their interesting chemical properties, offer opportunities for applications in biomedicine and physical chemistry. In this work, carbon K-edge absorption spectra of liquid perfluoroalkanes and their parent hydrocarbons are presented and compared. Based on soft X-ray absorption, a comprehensive picture of the electronic structure is provided with the aid of time dependent density functional theory. We have observed that conformational geometries mainly influence the chemical and electronic interactions in the presented liquid materials, leading to a direct association of conformational geometries to the dissolving capacity of the presented perfluorocarbons with other solvents like water and possibly gases like oxygen.
Shape-Controlled Synthesis of Pt Nanopeanuts
Authors: Zhang Xuemei , Xia Zengzilu , Huang Yingzhou , Jia Yunpeng , Sun Xiaonan , Li Yu , Li Xueming , Wu Rui , Liu Anping , Qi Xueqiang , Wang Shuxia , Wen Weijia 
Published: August 2016

AbstractExploring the novel shape of Pt nanoparticles is one of the most useful ways to improve the electrocatalytic performance of Pt in fuel cells. In this work, the Pt nanopeanuts consisting of two nanospheres grown together have been fabricated through a two-step polyol method. The high resolution scanning electron microscope (SEM) images and energy dispersive x-ray (EDX) spectrum collected at adjacent part point out the Pt nanopeanut is apparently different from the two physical attached nanospheres. To understand the growth mechanism of this nanopeanut, the final products in different synthesis situations are studied. The results indicate the interesting morphology of Pt nanopeanuts mainly benefit from the chemical reagent (FeCl3) while the size and homogeneity are greatly affected by the temperature. Furthermore, the electrocatalytic activity of the Pt nanopeanuts has also been demonstrated here. Our two-step synthesis of Pt nanopeanuts not only enlarges the group of Pt nanoparticles, but also provides a beneficial strategy for the synthesis of novel metal nanoparticles.
The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes
Authors: Estandarte Ana Katrina , Botchway Stanley , Lynch Christophe , Yusuf Mohammed , Robinson Ian 
Published: August 2016

AbstractChromatin undergoes dramatic condensation and decondensation as cells transition between the different phases of the cell cycle. The organization of chromatin in chromosomes is still one of the key challenges in structural biology. Fluorescence lifetime imaging (FLIM), a technique which utilizes a fluorophore’s fluorescence lifetime to probe changes in its environment, was used to investigate variations in chromatin compaction in fixed human chromosomes. Fixed human metaphase and interphase chromosomes were labeled with the DNA minor groove binder, DAPI, followed by measurement and imaging of the fluorescence lifetime using multiphoton excitation. DAPI lifetime variations in metaphase chromosome spreads allowed mapping of the differentially compacted regions of chromatin along the length of the chromosomes. The heteromorphic regions of chromosomes 1, 9, 15, 16 and Y, which consist of highly condensed constitutive heterochromatin, showed statistically significant shorter DAPI lifetime values than the rest of the chromosomes. Differences in the DAPI lifetimes for the heteromorphic regions suggest differences in the structures of these regions. DAPI lifetime variations across interphase nuclei showed variation in chromatin compaction in interphase and the formation of chromosome territories. The successful probing of differences in chromatin compaction suggests that FLIM has enormous potential for application in structural and diagnostic studies.