bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of computed tomography angiography to determine anterograde and collateral blood flow status in patients with symptomatic middle cerebral artery stenosis
Authors: Jia Baixue , Liebeskind David S , Song Ligang , Xu Xiaotong , Sun Xuan , Liu Lian , Wang Bo , Miao Zhongrong 
Published: March 2017

Background The purpose of this study was to determine the performance of computed tomography angiography (CTA) by using a scoring system to predict anterograde and collateral blood flow status in patients with symptomatic middle cerebral artery (MCA) stenosis with use of conventional angiography as standard reference. Methods We retrospectively identified all consecutive patients with unilateral symptomatic MCA stenosis in our center who underwent conventional angiography and CTA within 1 month. The anterograde and collateral blood flow (AnCo) scoring system consisted of anterograde score (AnS) and collateral score (CoS). Evaluation of the CTA images was done independently by two readers, based on the AnCo scoring system. The conventional angiography was assessed by using the Thrombolysis in Cerebral Infarction (TICI) and American Society of Interventional and Therapeutic Neuroradiology (ASITN/SIR) scoring system to determine the status of anterograde and collateral blood flow. Diagnostic performance of AnCo was evaluated by using the area under the receiver operating characteristic (ROC) curve. Results A total of 61 patients were included in the analysis with mean age of 53.4 ± 11.0 years. AnS demonstrated a strong correlation with TICI with statistical significance ( r = 0.786; p < 0.001). CoS had a modest yet statistically significant correlation with ASITN/SIR ( r = 0.574; p < 0.001). The ROC curve analysis for AnS demonstrated an area under the curve (AUC) of 0.894 ( p < 0.001) and the ROC curve analysis for CoS showed an AUC of 0.824 ( p < 0.001). Conclusions CTA was a potential method to evaluate anterograde and collateral blood flow status in patients with symptomatic unilateral MCA stenosis.
Up-regulation of CXCR4 expression contributes to persistent abdominal pain in rats with chronic pancreatitis
Authors: Zhu Hong-Yan , Liu Xuelian , Miao Xiuhua , Li Di , Wang Shusheng , Xu Guang-Yin 
Published: March 2017

Background Pain in patients with chronic pancreatitis is critical hallmark that accompanied inflammation, fibrosis, and destruction of glandular pancreas. Many researchers have demonstrated that stromal cell-derived factor 1 (also named as CXCL12) and its cognate receptor C-X-C chemokine receptor type 4 (CXCR4) involved in mediating neuropathic and bone cancer pain. However, their roles in chronic pancreatic pain remain largely unclear. Methods Chronic pancreatitis was induced by intraductal injection of trinitrobenzene sulfonic acid to the pancreas. Von Frey filament tests were conducted to evaluate pancreas hypersensitivity of rat. Expression of CXCL12, CXCR4, NaV1.8, and pERK in rat dorsal root ganglion was detected by Western blot analyses. Dorsal root ganglion neuronal excitability was assessed by electrophysiological recordings. Results We showed that both CXCL12 and CXCR4 were dramatically up-regulated in the dorsal root ganglion in trinitrobenzene sulfonic acid-induced chronic pancreatitis pain model. Intrathecal application with AMD3100, a potent and selective CXCR4 inhibitor, reversed the hyperexcitability of dorsal root ganglion neurons innervating the pancreas of rats following trinitrobenzene sulfonic acid injection. Furthermore, trinitrobenzene sulfonic acid-induced extracellular signal-regulated kinase activation and Nav1.8 up-regulation in dorsal root ganglias were reversed by intrathecal application with AMD3100 as well as by blockade of extracellular signal-regulated kinase activation by intrathecal U0126. More importantly, the trinitrobenzene sulfonic acid-induced persistent pain was significantly suppressed by CXCR4 and extracellular signal-regulated kinase inhibitors. Conclusions The present results suggest that the activation of CXCL12–CXCR4 signaling might contribute to pancreatic pain and that extracellular signal-regulated kinase-dependent Nav1.8 up-regulation might lead to hyperexcitability of the primary nociceptor neurons in rats with chronic pancreatitis.
Successful withdrawal from high-dose benzodiazepine in a young patient through electronic monitoring of polypharmacy: a case report in an ambulatory setting
Authors: Loscertales Hèctor R. , Wentzky Valerie , Dürsteler Kenneth , Strasser Johannes , Hersberger Kurt E. , Arnet Isabelle 
Published: March 2017

Background: Dependence on high-dose benzodiazepines (BZDs) is well known and discontinuation attempts are generally unsuccessful. A well established protocol for high-dose BZD withdrawal management is lacking. We present the case of withdrawal from high-dose lorazepam (>20 mg daily) in an unemployed 35-year-old male outpatient through agonist substitution with long-acting clonazepam and electronic monitoring over 28 weeks. Methods: All medicines were repacked into weekly 7 × 4 cavity multidose punch cards with an electronic monitoring system. The prescribed daily dosages of BZDs were translated into an optimal number of daily tablets, divided into up to four units of use. Withdrawal was achieved by individual leftover of a small quantity of BZDs that was placed in a separate compartment. Feedback with visualization of intake over the past week was given during weekly psychosocial sessions. Results: Stepwise reduction was obtained by reducing the mg content of the cavities proportionally to the leftovers, keeping the number of cavities in order to maintain regular intake behavior, and to determine the dosage decrease. At week 28, the primary objectives were achieved, that is, lorazepam reduction to 5 mg daily and cannabis abstinence. Therapy was continued using multidrug punch cards without electronic monitoring to maintain the management system. At week 48, a smaller size weekly pill organizer with detachable daily containers was dispensed. At week 68, the patient’s therapy was constant with 1.5 mg clonazepam + 5 mg lorazepam daily for anxiety symptoms and the last steps of withdrawal were started. Conclusions: Several key factors led to successful withdrawal from high-dose BZD in this outpatient, such as the use of weekly punch cards coupled with electronic monitoring, the patient’s empowerment over the withdrawal process, and the collaboration of several healthcare professionals. The major implication for clinical care is reduction by following the leftovers, and not a diktat from the healthcare professionals.
A phase 1 clinical trial of single-agent selinexor in acute myeloid leukemia
Authors: Garzon Ramiro , Savona Michael , Baz Rachid , Andreeff Michael , Gabrail Nashat , Gutierrez Martin , Savoie Lynn , Mau-Sorensen Paul Morten , Wagner-Johnston Nina , Yee Karen , Unger Thaddeus J. , Saint-Martin Jean-Richard , Carlson Robert , Rashal Tami , Kashyap Trinayan , Klebanov Boris , Shacham Sharon , Kauffman Michael , Stone Richard 
Published: March 2017

Abstract
Selinexor is a novel, first-in-class, selective inhibitor of nuclear export compound, which blocks exportin 1 (XPO1) function, leads to nuclear accumulation of tumor suppressor proteins, and induces cancer cell death. A phase 1 dose-escalation study was initiated to examine the safety and efficacy of selinexor in patients with advanced hematological malignancies. Ninety-five patients with relapsed or refractory acute myeloid leukemia (AML) were enrolled between January 2013 and June 2014 to receive 4, 8, or 10 doses of selinexor in a 21- or 28-day cycle. The most frequently reported adverse events (AEs) in patients with AML were grade 1 or 2 constitutional and gastrointestinal toxicities, which were generally manageable with supportive care. The only nonhematological grade 3/4 AE, occurring in &gt;5% of the patient population, was fatigue (14%). There were no reported dose-limiting toxicities or evidence of cumulative toxicity. The recommended phase 2 dose was established at 60 mg (∼35 mg/m2) given twice weekly in a 4-week cycle based on the totality of safety and efficacy data. Overall, 14% of the 81 evaluable patients achieved an objective response (OR) and 31% percent showed ≥50% decrease in bone marrow blasts from baseline. Patients achieving an OR had a significant improvement in median progression-free survival (PFS) (5.1 vs 1.3 months; P = .008; hazard ratio [HR], 3.1) and overall survival (9.7 vs 2.7 months; P = .01; HR, 3.1) compared with nonresponders. These findings suggest that selinexor is safe as a monotherapy in patients with relapsed or refractory AML and have informed subsequent phase 2 clinical development. This trial was registered at www.clinicaltrials.gov as #NCT01607892.
MeDeCom: discovery and quantification of latent components of heterogeneous methylomes
Authors: Lutsik Pavlo , Slawski Martin , Gasparoni Gilles , Vedeneev Nikita , Hein Matthias , Walter Jörn 
Published: March 2017

Abstract
It is important for large-scale epigenomic studies to determine and explore the nature of hidden confounding variation, most importantly cell composition. We developed MeDeCom as a novel reference-free computational framework that allows the decomposition of complex DNA methylomes into latent methylation components and their proportions in each sample. MeDeCom is based on constrained non-negative matrix factorization with a new biologically motivated regularization function. It accurately recovers cell-type-specific latent methylation components and their proportions. MeDeCom is a new unsupervised tool for the exploratory study of the major sources of methylation variation, which should lead to a deeper understanding and better biological interpretation.
Lacking of palladin leads to multiple cellular events changes which contribute to NTD
Authors: Tan Juan , Chen Xue-Jiao , Shen Chun-Ling , Zhang Hong-Xin , Tang Ling-Yun , Lu Shun-Yuan , Wu Wen-Ting , Kuang Ying , Fei Jian , Wang Zhu-Gang 
Published: March 2017

Abstract

Background
The actin cytoskeleton-associated protein palladin plays an important role in cell motility, morphogenesis and adhesion. In mice, Palladin deficient embryos are lethal before embryonic day (E) 15.5, and exhibit severe cranial neural tube and body wall closure defects. However, the mechanism how palladin regulates the process of cranial neural tube closure (NTC) remains unknown.

Methods
In this paper, we use gene knockout mouse to elucidate the function of palladin in the regulation of NTC process.

Results
We initially focuse on the expression pattern of palladin and found that in embryonic brain, palladin is predominantly expressed in the neural folds at E9.5. We further check the major cellular events in the neural epithelium that may contribute to NTC during the early embryogenesis. Palladin deficiency leads to a disturbance of cytoskeleton in the neural tube and the cultured neural progenitors. Furthermore, increased cell proliferation, decreased cell differentiation and diminished apical cell apoptosis of neural epithelium are found in palladin deficient embryos. Cell cycle of neural progenitors in Palladin

-/-
embryos is much shorter than that in wt ones. Cell adhesion shows a reduction in Palladin

-/-
neural tubes.

Conclusions

Palladin is expressed with proper spatio-temporal pattern in the neural folds. It plays a crucial role in regulating mouse cranial NTC by modulating cytoskeleton, proliferation, differentiation, apoptosis, and adhesion of neural epithelium. Our findings facilitate further study of the function of palladin and the underlying molecular mechanism involved in NTC.
Mental health among young adults in prison: The importance of childhood-onset conduct disorder
Authors: Hofvander Björn , Anckarsäter Henrik , Wallinius Märta , Billstedt Eva 
Published: March 2017

BackgroundThe psychiatric health burden of prisoners is substantial. However, there is a lack of high-quality studies of psychiatric disorders among young adults with a high risk of reoffending.AimsTo investigate the lifetime prevalence of psychiatric disorders and use of mental health services among young male violent offenders and the impact of childhood-onset conduct disorder (COCD).MethodA nationally representative cohort (n= 270, age 18–25) of male offenders was followed back in medical records and clinically assessed by gold standard methods. Lifetime prevalences are presented together with odds ratios (ORs) as risk estimates in relation to COCD.ResultsPrevious use of psychiatric services among the participants was high but their lifetime psychiatric morbidity was even higher, with 93% meeting criteria for at least one Axis I disorder. The COCD group was overrepresented in most clinical categories and carried five times higher odds (OR = 5.1, 95% CI 2.0–12.8) of a psychotic disorder, three times higher odds (OR = 3.2, 95% CI 1.2–8.5) of a substance use disorder and two times higher odds of a mood disorder (OR = 2.3, 95% CI 1.3–4.0) or anxiety disorder (OR = 2.0, 95% CI 1.1–3.5).ConclusionsThe mental health burden is substantial among young violent offenders, and COCD is an important indicator of future mental health problems which must be a priority for public health efforts.
An ELISA DYRK1A non-radioactive kinase assay suitable for the characterization of inhibitors
Authors: Liu Yong , Adayev Tatyana , Hwang Yu-Wen 
Published: March 2017

The DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1A) gene encodes a proline-directed Ser/Thr kinase. Elevated expression and/or altered distribution of the kinase have been implicated in the neurological impairments associated with Down syndrome (DS) and Alzheimer’s disease (AD). Consequently, DYRK1A inhibition has been of significant interest as a potential strategy for therapeutic intervention of DS and AD. Many classes of novel inhibitors have been described in the past decade. Although non-radioactive methods for analyzing DYRK1A inhibition have been developed, methods employing radioactive tracers are still commonly used for quantitative characterization of DYRK1A inhibitors. Here, we present a non-radioactive ELISA assay based on the detection of DYRK1A-phosphorylated dynamin 1a fragment using a phosphorylation site-specific antibody. The assay was verified by the use of two well-characterized DYRK1A inhibitors, epigallocatechin gallate (EGCG) and harmine. The IC50s for EGCG and harmine determined by the ELISA method were found to be comparable to those previously measured by radioactive tracing methods. Furthermore, we determined the mode of inhibition for EGCG and harmine by a modification of the ELISA assay. This assay confirms the mode of inhibition of EGCG (non-ATP-competitive) and harmine (ATP-competitive), as previously determined. We conclude that the ELISA platform demonstrated here is a viable alternative to the traditional radioactive tracer assays for analyzing DYRK1A inhibitors.
In-Store Advertising by Competitors
Authors: Kuksov Dmitri , Prasad Ashutosh , Zia Mohammad 
Published: March 2017

Conventional advice to firms in competitive markets is to raise barriers against competitive poaching of their customers. However, we see instances where a firm enables competitor advertising to its customers. For example, Walmart hosts banner ads for TVs from Sears to customers searching for TVs on Walmart.com, risking a loss of customers in exchange for a commission. This paper explores whether and under what conditions allowing competitor advertising in one’s store may be a beneficial strategy. We analyze a duopoly market where customers are heterogeneous in search costs, information, and preferences. We find that hosting a competitor ad for an undifferentiated product can mitigate price competition and boost profits of both firms if the advertising commission is high enough. Otherwise, hosting competitor advertising may decrease the profits of both firms. Thus, there is no conflict of interest between firms in advertising and in setting the ad commission level. Yet the host prefers more efficient ads while the advertiser does not. Furthermore, the equilibrium outcome is asymmetric, with only one store featuring ads of the other. If stores are sufficiently differentiated in marginal costs of the product, the cost disadvantaged store will be the host. We show that the results are robust to displaying price in the ad, to different commission structures, and to customer uncertainty about the commission rate. The online appendix is available at https://doi.org/10.1287/mksc.2016.1015 .
The diversity of undergrowth plants on Acacia nilotica stands as food resources of banteng (Bos javanicus) in Baluran National Park, East Java, Indonesia
Authors: DJUFRI DJUFRI , WARDIAH WARDIAH 
Published: March 2017

Abstract. Djufri, Wardiah. 2017. The diversity of undergrowth plants on Acacia nilotica stands as food resources of banteng (Bos javanicus) in Baluran National Park, East Java, Indonesia. Biodiversitas 18: 288-294. The objectives of this research were to determine species composition, importance value of species, diversity index and evenness index of the undergrowth plants of Acacia nilotica stands. The quadratic method was used in this study. The similarity indeks and cluster analysis were performed to classify the community structure of undergrowth plants of Acacia nilotica. The results of this study showed that there were 63 species of plant belonging to 18 families. The importance value ranged between 0.97-42.58 and high and medium importance values belonged to Brachiaria reptans, Thespesia lampas, Oplismenus burmannii, Dichanthium coricosum, Axonopus compressus, and Synedrella nodiflora. The diversity index and evenness ranged 1.1504-2.7556 and 1.1067-1.7854, respectivelly. The Brachiaria reptans, Dichantium coricosum, Thespesia lanpas, Achyrantes aspera, Stachytarpeta indica, Axonopus compressus and Oplismenus burmanii were predominant in study area. In addition the invasion of A. nilotica has decreased the carryng capacity of savanna in Baluran National Park to the banteng.
Keywords: Acacia nilotica, species composition, banteng, Baluran National Park
Morphological variations and sex expression in gametophytes of Cibotium barometz under in vitro conditions
Authors: PRAPTOSUWIRYO TITIEN NGATINEM , ISNAINI YUPI 
Published: March 2017

Abstract. PraptosuwiryoTNg, Isnaini Y. 2017. Morphological variations and sex expression in gametophytes of Cibotium barometz under in vitro conditions. Biodiversitas 18: 312-320. Characteristics of gametophytes in ferns have been shown to be phylogenetically significant. Studies of fern gametophytes have become essential to fill the gaps in our knowledge of fern morphology, ecology, reproduction, evolution and distribution. The purposes of this study were: (i) to observe morphological variations and sex expression in gametophytes of Cibotium barometz (L.) J. Sm., and (ii) to understand how gametophyte densities affect sex expression in the species. Spores of five collection numbers of C. barometz from Sumatra, Indonesia, were sown on half-strength Murashige & Skoog (Â½MS) basal medium. Eleven weeks after germination of spores, prothalli were subcultured on Â½MS medium along with sugar (30 g/L) and Naphthalene Acetic Acid (NAA, 0.5 mg/L). After eight months subculturing of prothalli (prothallus density: 100-150 individuals per cm2), 100 gametophytes were observed for each collection number to determine their shapes and sex expression. Between 9- 12 months after subculturing, gametophytes growing at different population densities (between 100-500 individuals per cm2) were sampled. One hundred prothalli were selected among the ten replicates for each collection number. The percentage of each identified gametophyte shape and their genders were recorded. Eight morphological types of adult gametophyte were recorded: (i) Branching filament (asexual), (ii) ribbon-like shape (male), (iii) spatulate shape (asexual, male, female), (iv) heart shape (male, female, bisexual), (v) gemmiferous heart shape (asexual, female, bisexual), (vi) long heart shape (male, bisexual); (vii) gemmiferous long heart shape (asexual, male, bisexual), and (viii) gemmiferous irregular shape (asexual, male, female, bisexual). We conclude that gametophyte morphology is simply â€œtoo plasticâ€ to be used in suporting species delimitation in ferns if the prothalli is to be cultured in a high population density. There is a correlation between gametophyte size, shape and sex expression that is related to the population density. The presence of unisexual and bisexual gametophytes indicates that both intergametophytic and intragametophytic selfing occur in C. barometz.
Keywords: Cibotium barometz, Environmental Sex Determination theory, gametophyte, morphology, sex expression
Short Communication: Waterbird species distribution between natural and manmade wetland in Himalayan foothills of Uttarakhand, India
Authors: SAINI VIKAS , JOSHI KAMAL , BHATT DINESH , SINGH ASHUTOSH , JOSHI RITESH 
Published: March 2017

Abstract. Saini V, Joshi K, Bhatt D, Singh A, Joshi R. 2017. Short Communication: Waterbird species distribution between natural and manmade wetland in Himalayan foothills of Uttarakhand, India. Biodiversitas 18: 334-340. A comparative study on waterbird diversity and abundance was conducted at natural and man-made wetland of District Hardwar from 2010 to 2013. A total of 37 waterbirds belonging to 11 families were recorded of which 14 species were winter migrant in the wetlands of the study area. Among these waterbird species, two species viz. Black-necked Stork, Ephippiorhynchus asiaticus; River Lapwing, Vanellus duvaucelii were near threatened (IUCN status Ver. 2013.1.) and two species namely Woolly-necked Stork, Ciconia episcopus; andÂ Marbled Duck, Marmaronetta angustirostris were Vulnerable (IUCN status). The avian species diversity and abundance were recorded significantly high (t = 4.16, p < 0.01) at natural wetland site. It is also observed that vegetation variety and food availability is the responsible causes of waterbird species variation in the natural and manmade wetland. The results of this study suggest that freshwater natural wetland site is more suitable habitat for short and long-distance water migratory birds. This natural wetland should be protected to enhance the abundance and diversity of water migrant community.
Keywords: Diversity, man-made wetland, migratory species, natural wetland