bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polyprenylated Benzophenones from Brazilian Red Propolis: Analytical Characterization and Anticancer Activity
Authors: Pires Loren M. , Santos Mario F. C. , Figueiredo Laisla R. , Faleiros Renata , Badoco Fernanda R. , Silva Karolinne B. , Ambrósio Sérgio R. , Bastos Jairo Kenupp , dos Santos Raquel A. , Veneziani Rodrigo C. S. 
Published: September 2024

AbstractThe present work describes the extraction of a polyprenylated benzophenone‐rich extract from Brazilian red propolis (ERPB), the development and validation of an RP‐HPLC‐UV method to characterize it, and its evaluation against breast cancer cell lines MCF‐7 and MDA‐MB‐231, as well as the normal counterpart MCF‐10 A. A mixture of gutifferone E and xanthochymol (1+2), and isolated oblongifolin B (3) were used as chemical standards for ERPB and were also evaluated. The concentrations of 1+2 and 3 corresponded to 16.68 % and 42.25 % of the total content of the extract, respectively, and the validation parameters evaluated were satisfactorily met. The cytotoxic effects of ERPB were assessed, and the obtained IC50 values were 19.58 μg/mL (MCF‐10 A), 11.56 μg/mL (MCF‐7), and 5.22 μg/mL (MDA‐MB‐231). In conclusion, ERPB exhibits promising cytotoxic effects on the tested breast cell lines. However, further investigation to elucidate its potential therapeutic applications and safety profile should be conducted.
Five Undescribed Sesquiterpenes with Anti‐Inflammasome Activity Isolated from Saussurea Laniceps
Authors: Zu Xuedan , Ding Ling , Zheng Bowen , Liang Zhongdan , Li Yan , Wu Xuewen , He Han , Zhang Xingjie , Li Xiaoli , Xiao Weilie 
Published: September 2024

AbstractFive previously undescribed guaialactone‐type sesquiterpenes (1–5), called Lanicepsmines A–E, along with eleven know compounds (6–16), were isolated from the whole plant of Saussurea laniceps. NMR spectroscopic data analysis and MASS permitted the definition of their structures. The stereochemistry of the compounds was confirmed by ECD calculations. It is worth noting that compound 1 and 2 contain amino groups. Part of compounds were tested the anti‐inflammasome activity, and compound 14 exhibited potent activity and decreased LDH level in a dose‐dependent manner, with IC50 values of 0.698 μM.
Eco‐sustainable Synthesis and Potential Efficiency of Some Novel N‐containing Heterocyclic Derivatives as Insecticidal and Photosensitizing Agents Against Musca domestica L.
Authors: Hassan A. M. A. , Abubshait Samar A. , Abdel‐Haleem Doaa R. , El‐Naggar Abeer M. , Hassaballah Aya I. 
Published: September 2024

AbstractThe rising application of conventional synthetic insecticides develops resistant populations of houseflies; therefore, using new chemical agents with different modes of action is essential to overcome this problem. The mechanical grinding technique was used as a green method, to synthesize the tested compounds because it is a more facile work‐up and high‐yield economy, simplicity and solvent‐free than conventional thermal technique. Various methods were employed to synthesize new heterocycles containing anthracene (a photosensitizing agent) from chalcone 3, a building block material such as the preparation of the pyrazole derivatives 4–7, isoxazole derivative 8, pyrimidines 9–11, and oxirane derivative 12. The novel synthesized compounds were analyzed by FT‐IR, 1H‐NMR, 13C‐NMR spectra, and elemental analysis. Herein, the toxicity of the anthracene derivatives was assessed against Musca domestica larvae and adults in different conditions to demonstrate the effect of various inserted moieties on the efficiency of tested compounds. Furthermore, the influence of sunlight on the toxicity of anthracene was studied in dark and sunlight tests against adult houseflies. Moreover, these compounds diminished the total protein and lipids contents while significantly influencing the antioxidant enzymes activities of M. domestica adults. Structure‐activity relationships demonstrated the role of each moiety on the toxicity of compounds.
Development of chitosan‐based nanoparticles encapsulating Bacillus velezensis CMRP4490 metabolites for enhanced in vitro control of Sclerotinia sclerotiorum
Authors: de França Silva de Lima Gesiara , Michele Suzuki Rúbia , de Oliveira Junior Admilton Gonçalves , Aranha Ana Caroline Raimundini , Defendi Rafael Oliveira , Gomes Maria Carolina Sérgi , Nicoletto Maria Luíza Abreu , Casagrande Sipoli Caroline 
Published: September 2024

AbstractModern agriculture seeks to control pests and diseases in the field while maintaining production, reducing the use of dangerous chemical molecules, and resorting to more sustainable solutions. One of the ways to achieve these objectives is biological control. Furthermore, combined with biological control, the use of nanoencapsulation techniques of biological control agents with materials that are bioavailable and biodegradable in the environment has proven to be an alternative to reducing the use of non‐renewable materials. Therefore, the present work aimed to develop a nanoparticle system for biological control of Sclerotinia sclerotiorum. The nanoparticle system was produced using the ionotropic gelation technique using chitosan (CHI) as a polymer. The cell‐free supernatant (CFS) of the microorganism Bacillus velezensis CMRP4490 was used to produce nanoparticles, as preliminary studies show that its metabolites act in biological control. The nanoparticles produced were prepared in different concentrations of CHI and CFS and their antimicrobial activity was evaluated against the fungus S. sclerotiorum. The encapsulated samples have a concentration of 20%–80% of CFS and 0.25% and 0.8% of CHI and showed a 100% inhibitory effect against S. Sclerotiorum, and the results obtained indicate a synergistic effect between CHI and CFS.
Redox‐Neutral Nickel‐Catalyzed Selective Hydroalkynylation of Internal Alkyne and Its Application in Anticancer Agent Discovery†
Authors: Chen Weiming , Liu Teng , Li Shuqing , Li Guangyu , Wu Gaorong , Gao Youjia , Xu Zhilin , Wu Yitao , Peng Xiaopeng , Huang Jiuzhong 
Published: September 2024

Comprehensive SummaryHerein, an unprecedented nickel‐catalyzed regioselective hydroalkynylation of unsymmetrical internal alkynes was realized with steric hindered resistance selectivity via the cyano‐directing group strategy. Significantly, the resulting 1,3‐enyne products could be effectively employed in the synthesis of novel nitrogen‐containing tricyclics compounds, that provided the potential candidate compound 8a (IC50 = 2.6—6.1 μmol/L) for the anti‐tumor cell proliferation activity. Therefore, this work not only improves the transition‐metal‐ catalyzed hydroalkynylation strategy of internal alkynes, but also exhibits versatility of 1,3‐enynes in the construction of the complex bioactive chemical space.
Impact of Immediate Versus Staged Complete Revascularization on Short‐Term and Long‐Term Clinical Outcomes in Patients With Acute Coronary Syndrome and Multivessel Disease: A Systematic Review and Meta‐Analysis
Authors: Jia Qiufeng , Zuo Ankai , Zhang Chengrui , Yang Danning , Zhang Yu , Li Jing , An Fengshuang 
Published: September 2024

ABSTRACTBackgroundIn patients with acute coronary syndrome (ACS) and multivessel disease (MVD), complete revascularization (CR) improves prognosis. This meta‐analysis, summarizing recent RCTs, contrasts short‐term and long‐term clinical outcomes between immediate complete revascularization (ICR) and staged complete revascularization (SCR).MethodsWe systematically searched the online database and eight RCTs were involved. The primary outcomes included long‐term unplanned ischemia‐driven revascularization, re‐infarction, combined cardiovascular (CV) death or myocardial infarction (MI), all‐cause death, CV death, stroke, and hospitalization for heart failure (HHF). The secondary outcomes were 1‐month unplanned ischemia‐driven revascularization, re‐infarction, all‐cause death, and CV death. Safety endpoints included stent thrombosis and major bleeding.ResultsEight RCTs comprising 5198 patients were involved. ICR reduced long‐term unplanned ischemia‐driven revascularization (RR 0.64, 95% CI 0.51–0.81, p < 0.001), combined CV death or MI (HR 0.51, 95% CI 0.34–0.78, p = 0.002), and re‐infarction (RR 0.66,95% CI 0.48 to 0.91, p = 0.012) compared with SCR. ICR also decreased 1‐month unplanned ischemia‐driven revascularization (RR 0.41, 95% CI: 0.21–0.77, p = 0.006) and re‐infarction (RR 0.33, 95% CI:0.15–0.74, p = 0.007) but increased 1‐month all‐cause death (RR 2.22, 95% CI 1.06–4.65, p = 0.034).ConclusionIn ACS patients with MVD, we first found that ICR significantly lowered the risk of both short‐term and long‐term unplanned ischemia‐driven revascularization and re‐infarction, as well as the long‐term composite outcome of CV death or MI compared with SCR. However, there may be an increase in 1‐month all‐cause death in the ICR group.
Step‐By‐Step Procedure to Identify Previously Unknown Compounds by LC‐QTOF‐MS Exemplified by an Intoxication With the Methaqualone Analog SL‐164
Authors: Fels Helena , Franz Simon , Dame Torsten , Skopp Gisela , Musshoff Frank 
Published: September 2024

ABSTRACTIn September 2019, a 22‐year‐old man with a history of drug abuse presented to the hospital with altered mental status. Due to a suspected drug overdose, a blood sample taken on admission and a urine sample collected 30 h thereafter were submitted to our laboratory to test for illegal drugs, pharmaceutical substances, and designer drugs. During the routine toxicological analysis of the serum sample, morphine and phenobarbital were identified by liquid chromatography‐quadrupole‐time‐of‐flight mass spectrometry (LC‐QTOF‐MS). Additionally, two compounds showing identical accurate masses and isotope ratios as the designer benzodiazepine diclazepam and the benzodiazepine lormetazepam were found. However, retention times differed significantly from the expected values, and the acquired MS/MS spectra did not match the library entries of the two compounds, indicating the presence of two previously unknown substances. After further investigation, SL‐164 (5‐chloro‐3‐(4‐chloro‐2‐methylphenyl)‐2‐methyl‐4(3H)‐quinazolinone), a methaqualone analog, which has recently emerged on the research chemical market, and its hydroxy metabolite were tentatively identified by accurate mass, isotope matching, and plausible fragmentation. However, for unequivocal confirmation and quantification, a reference standard is required. As no reference material was available by the end of 2019, SL‐164 was obtained from an online shop, and its identity and purity (97.8%) were confirmed by nuclear magnetic resonance spectroscopy. The subsequent quantitative analysis revealed a concentration of 390 ng/mL SL‐164 in serum. In the urine sample, the parent compound was not detected, but three suspected monohydroxylated metabolites were found. This example shows that LC‐QTOF‐MS is a powerful approach for the (tentative) identification of unknown compounds in biological matrices.
Effects of functional composition on plant competitors, stress‐tolerators, ruderals ecological strategies in forest communities across different climatic zones
Authors: Han Xin , Yao Jie , Wang Ruixue , Xu Yue , Huang Jihong , Ding Yi , Zang Runguo 
Published: September 2024

AbstractEcological strategies identified by plant functional traits are valuable descriptors for understanding species, populations, communities, and ecosystems in response to environmental conditions. Ecological strategies, in conjunction with the functional structure of plant communities, serve as crucial tools for investigating complex relationships among the environment, vegetation, and ecosystem functions. However, it remains unclear whether the functional structure (specifically, community‐weighted mean [CWM] traits) accurately reflects the optimal ecological strategies in forest communities. Here, we gathered seven functional traits for each species from four distinct forest vegetation types across four climatic zones, including leaf area (LA), specific leaf area (SLA), leaf dry matter content (LDMC), leaf phosphorus concentration (LPC), leaf nitrogen concentration (LNC), wood density (WD) and maximum plant height (H). We based on CSR (Competitors, Stress‐tolerators, Ruderals) theory and “StrateFy” ordination method utilizing LA, LDMC and SLA to position them within CSR triangle and categorize them into four ecological strategy groups: Competitive, Stress‐tolerant, Intermediate, and Ruderal ecological strategy groups (C‐group, S‐group, Int‐group, and R‐group). We then determined the proportion of species in each group. Subsequently, we calculated the CWM trait values for the remaining four functional traits: WD (CWM‐WD), LPC (CWM‐LPC), LNC (CWM‐LNC) and H (CWM‐H). Non‐metric multidimensional scaling and hierarchical partitioning revealed that CWM‐WD, CWM‐LPC, CWM‐LNC and CWM‐H significantly influenced the ecological strategies of forest communities. The synergistic interaction of CWM‐WD and CWM‐LPC had the most significant impact on ecological strategies within forest communities. Notably, CWM‐WD emerged as the most crucial single CWM trait for explaining variation in ecological strategies within forest communities. In conclusion, our study demonstrates that CWM traits effectively reflect optimal CSR ecological strategies in forest communities across different climatic zones, with CWM‐WD serving as a preferred indicator. This can improve our critical insights into key ecological processes in forest communities using trait‐based approach.
Nanoencapsulation of wheat germ oil with chitosan‐nettle seed gum‐ovalbumin: Preparation, optimization, and characterization
Authors: Kutlu Gözde , Akman Perihan Kubra , Erol Kübra Feyza , Bozkurt Fatih , Tornuk Fatih 
Published: September 2024

AbstractThis study was aimed to optimize nanoencapsulation conditions of ovalbumin (OV)‐nettle seed gum (NSG)‐chitosan (CS), ONC‐based nanocarriers, using response surface methodology (RSM). Then, wheat germ oil (WGO) was encapsulated in optimized ONC nanocarriers, and WGO‐loaded nanocarriers were characterized for their encapsulation efficiency, zeta potential, average particle size, emulsion stability, thermal, structural, micromorphological, rheological, and cytotoxic properties. The results showed that NSG‐CS amount of 30 mL, OV amount of 20 mL, and pH of 3 gave the maximum zeta potential and minimum average particle size based on the predicted conditions for the production of ONC nanocarriers by RSM. Encapsulation efficiencies of WGO‐loaded nanoparticles (WGONs) containing different ratios of WGO (2.5% to 4.5%, v:w) ranged from 48.66% to 71.91%. According to rheological properties, both the solutions of ONC complex and WGONs exhibited Newtonian flow behavior. The FT‐IR spectra exhibited changes in the peaks associated with amino and carboxyl groups following the encapsulation of WGO, suggesting the successful completion of the encapsulation process. SEM images revealed that the addition of WGO into the capsule resulted in the formation of rough particles. The cytotoxicity tests demonstrated that concentrations of WGO, CS, NSG, OV, WGO, ONC, and WGON up to 10 mg mL–1 did not significantly reduce the viability of L‐929 human fibroblast cell lines, indicating their nontoxicity toward healthy cells. In conclusion, this study suggested the successful encapsulation of WGO within ONC carriers at the nanoscale, achieving high encapsulation efficiency and favorable properties to enhance their uses for different purposes.Practical Application: Wheat germ, an important by‐product of wheat milling industry, contains considerable amounts of oil which possesses many beneficial components like tocopherol, vitamin B, phytosterols, and policosanols. These compounds have potential health benefits such as supporting exercise performance, cardiovascular health, and reducing the risk of overweight/obesity. Therefore, this underutilized oil needs to be introduced to the food/nutraceutical industry more extensively. However, the susceptibility of wheat germ oil (WGO) to oxidation and its short shelf‐life limit its further use. Encapsulation technology can be employed to protect the WGO, extend its shelf‐life, and improve its stability. Response surface methodology (RSM) is also a versatile way to determine the optimal conditions for encapsulation, resulting in the formation of stable nanocapsules containing WGO. The resulting nanocapsules effectively release the active ingredients of WGO, protecting them from oxidation and making them highly suitable for use as functional food ingredients or in pharmaceutical products with promising potential.
Integrating computational thinking practices into early childhood education in culturally responsive ways: Insights from research–practice partnership
Authors: Hubelbank Jeanne , Dubosarsky Mia , Kayumova Shakhnoza , Davis Tiffany , Sann Nea , Fortin Shawn , Smith Gillian 
Published: September 2024

AbstractThe study examines the research–practice partnership (RPP) model aimed at co‐constructing and integrating computational thinking (CT) in culturally responsive (CR) ways within early childhood and elementary (PreK‐5) classrooms, focusing on teachers' RPP experiences. Recent research underlines the importance of integrating CT in early childhood and elementary education, demonstrating that culturally, linguistically, and developmentally responsive computational activities in PreK‐5 settings are key to such efforts. However, there is a gap in research regarding effective professional development models that would prepare early childhood teachers in terms of both disciplinary ideas and practices of CT, as well as attending to cultural, linguistic, and developmental differences in young children in asset‐based ways. Our research contributes to this body of knowledge by examining how RPP can be a model for teacher learning in which teachers, administrators, and researchers can co‐develop knowledge and confidence in integrating CT into the PreK‐5 teaching curriculum and practices in CR ways. The study outlines the collaborative development of processes and frameworks co‐constructed by the RPP educators, as well as teacher‐developed curricular materials and lesson plans integrating CT in CR ways. Findings include teachers' experience of these lessons and teacher insights about the support needed to incorporate CT and CR practices into their existing curricula. The study concludes by highlighting the potential challenges and opportunities inherent in such endeavors, thereby contributing to the broader discourse on supporting CT integration into early childhood classrooms in CR ways.
An assessment of future climatic and anthropogenic impacts on the hydrological system of a semi‐arid catchment
Authors: Nune Rajesh , Western Andrew W. , George Biju A. , Gummadi Sridhar , Pasupuleti Srinivas , Ragab Ragab , Dixit Sreenath 
Published: September 2024

AbstractClimate and catchment characteristics, particularly land and water use and management, may vary according to the population growth rate, future food habits and water demands. Three climate simulations corresponding to the Intergovernmental Panel on Climate Change, Special Report on Emissions Scenarios (A1B) were downscaled using the ‘Providing Regional Climates for Impact Studies’ (PRECIS) for the period 1961–2098, and bias correction was performed using the quantile mapping (QM) method. A semi‐distributed integrated model (Modified Soil and Water Assessment Tool, SWAT) was used to predict the impacts of dynamic changes in catchment characteristics in the Himayat Sagar (HS) catchment and the effects of future climate change on future streamflow and groundwater storage. Simulations predicted that if this trend continues in the future, future climate and anthropogenic changes will lead to a more than 50% reduction in streamflow and a 50% increase in actual evaporation in the HS catchment. This would reduce groundwater storage to a depth of 15 m compared to current conditions, and by the end of the century, there would be no contribution from the base flow to the streamflow. Overall, unless current policies are modified to stabilize land and water management practices, anthropogenic changes will have greater importance than climate change.
Improved understanding of soil water content at field capacity and estimates from pedotransfer functions
Authors: Andabili Samira Jaefarzade , Rasoulzadeh Ali , Moghadam Javad Ramezani , Pollacco Joseph Alexander Paul , Fernández‐Gálvez Jesús 
Published: September 2024

AbstractThe soil water content at field capacity, θFC, is a fundamental variable for irrigation and agriculture. This study determines the optimal tension (−33 or −10 kPa) of deriving θFC which best matches the in situ field measurements following the Veihmeyer procedure. The Veihmeyer method refers to a profile water status for which there is a negligible drainage rate of bare soil without evaporation. In addition, we derive a set of linear and nonlinear pedotransfer functions (PTFs) which estimate θFC. θFC was measured in 69 plots in north‐western Iran, which has a cold semi‐arid climate. The soil properties used for developing PTFs include texture, bulk density, and organic matter. The results show that θFC cannot be derived at a fixed tension. We therefore developed PTFs with satisfactory performance in reproducing in situ measured θFC. The findings show that PTFs developed at a fixed tension consistently underestimate θFC derived in situ. It was also speculated that tension less than −10 kPa could yield improved predictions of in situ θFC.
Pathogenicity and transcriptomic resolution in dengue virus serotype 1 infected AGB6 mouse model
Authors: Yu Ning , Chen Shigang , Liu Yumeng , Wang Peng , Wang Longlong , Hu Ningning , Zhang He , Li Xiao , Lu Huijun , Jin Ningyi 
Published: September 2024

AbstractDengue viruses are the causative agents of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome, which are mainly transmitted by Aedes aegypti and Aedes albopictus mosquitoes, and cost billions of dollars annually in patient treatment and mosquito control. Progress in understanding DENV pathogenesis and developing effective treatments has been hampered by the lack of a suitable small pathological animal model. Until now, the candidate vaccine, antibody, and drug for DENV have not been effectively evaluated. Here, we analyzed the pathogenicity of DENV‐1 in type Ⅰ and type Ⅱ interferon receptor‐deficient mice (AGB6) by intraperitoneal inoculation. Infected mice showed such neurological symptoms as opisthotonus, hunching, ataxia, and paralysis of one or both hind limbs. Viremia can be detected 3 days after infection. It was found that 6.98 × 103 PFU or higher dose induce 100% mortality. To determine the cause of lethality in mice, heart, liver, spleen, lung, kidney, intestinal, and brain tissues were collected from AGB6 mice (at an attack dose of 6.98 × 103 PFU) for RNA quantification, and it was found that the viral load in brain tissues peaked at moribund states (14 dpi) and that the viral loads in the other tissues and organs decreased over time. Significant histopathologic changes were observed in brain tissue (hippocampal region and cerebral cortex). Hematological analysis showed hemorrhage and hemoconcentration in infected mice. DENV‐1 can be isolated from the brain tissue of infected mice. Subsequently, brain tissue transcriptome sequencing was performed to assess host response characteristics in infected AGB6 mice. Transcriptional patterns in brain tissue suggest that aberrant expression of pro‐inflammatory cytokines induces antiviral responses and tissue damage. Screening of hub genes and their characterization by qPCR and ELISA, it was hypothesized that IL‐6 and IFN‐γ might be the key factors in dengue virus‐induced inflammatory response. Therefore, this study provides an opportunity to decipher certain aspects of dengue pathogenesis further and provides a new platform for drug, antibody, and vaccine testing.