bluuebunny/crossref_metadata_2025_split huggingface dataset:
7871 Investigating The Impact Of Prenatal Exposure To A Mix Of Endocrine-Disrupting Chemicals On Development: Behavioral, Physiological, And Transcriptomic Analyses
Authors: Streifer Madeline R , Thompson Lindsay , Gore Andrea C 
Published: October 2024

Abstract
Disclosure: M.R. Streifer: None. L. Thompson: None. A.C. Gore: None.
In recent decades, there has been a growing concern surrounding the presence of endocrine-disrupting chemicals (EDCs) in our environment and their potential implications on human health. Among these, prenatal exposure and subsequent effects on the developing neuroendocrine system have emerged as a subject of interest. This study aims to define the long term developmental, behavioral, and molecular effects of prenatal exposure to NeuroMix, a complex mixture of environmentally relevant EDCs. To this end, pregnant rat dams were fed either NeuroMix or vehicle, exposing the developing fetuses during critical periods of neuroendocrine development. The exposed offspring (F1 generation) were the experimental subjects, which were allowed to mature to adulthood, underwent behavioral testing, then were euthanized for brain tissue collection. Our study revealed noteworthy sex-specific effects, whereby exposed males exhibited reduced bodyweight and anogenital index, as well as delayed puberty. In contrast, females displayed no overt developmental abnormalities but exhibited heightened risk-taking behaviors in controlled behavioral experiments like the light:dark box. To unravel the molecular underpinnings of these observed effects, 3’ Tag-sequencing and transcriptomic analyses were performed on the arcuate nucleus (ARC) and ventromedial nucleus (VMN), two hypothalamic nuclei involved in hormonal regulation, metabolism, fear-based behaviors, and development. Results of Ingenuity Pathway Analysis (IPA) unveiled differentially expressed genes in both males and females, highlighting key pathways implicated in mitochondrial dysfunction and estrogen receptor signaling. Other notable pathways identified were related to cancer, neurological disorders, and endocrine system disorders. This comprehensive investigation highlights the impact of prenatal exposure to EDCs, influencing not only physiological parameters like body weight and puberty onset, but also behavioral phenotypes indicative of neurobiological alterations. The identification of disrupted molecular pathways provides valuable insights into the potential mechanisms linking EDC exposure to adverse health outcomes, warranting further exploration and consideration in the context of human health and environmental policy. Grant support: R01 ES029464.
Presentation: 6/1/2024
7899 Mixture Of Urinary Concentrations Of Phenols And Phthalate Metabolites In Relation To Serum Levels Of Lipid Biomarkers Among Pregnant Women: Results From The EARTH Study
Authors: Shen Xilin , Génard-Walton Maximilien , Williams Paige L , James-Todd Tamarra , Ford Jennifer B , Rexrode Kathryn M , Zhang Dan , Chavarro Jorge E , Hauser Russ B , Alarcon Lidia Minguez 
Published: October 2024

Abstract
Disclosure: X. Shen: None. M. Génard-Walton: None. P.L. Williams: None. T. James-Todd: None. J.B. Ford: None. K.M. Rexrode: None. D. Zhang: None. J.E. Chavarro: None. R.B. Hauser: None. L. Minguez Alarcon: None.
Given the limited evidence on endocrine-disrupting chemicals (EDCs), evaluated as mixture, in relation to lipid biomarkers during pregnancy, we examined whether mixtures of urinary concentrations of bisphenol A (BPA), parabens and phthalate metabolites were associated with serum lipid levels among 175 pregnant women who enrolled in Environment and Reproductive Health (EARTH) Study from 2005 to 2017. The outcomes were assessed at one random timepoint pre women during pregnancy, including serum levels of total triglycerides, total cholesterol, high-density lipoprotein (HDL), non-HDL, and low-density lipoprotein (LDL). We grouped the chemical biomarkers [di(2-ethylhexyl) phthalate (DEHP) metabolites and BPA; other phthalate metabolites; parabens] assessed in urine collected at the same time and applied Bayesian Kernel Machine Regression (BKMR) and quantile g-computation to evaluate the associations while adjusting for confounders [urine specific gravity, age, pre-pregnancy body mass index (BMI), race, education level, infertility diagnosis, mode of conception, number of babies in this pregnancy and trimester]. In the adjusted BKMR models, we found no associations between the overall chemical biomarker mixture and lipid levels for example total cholesterol [mean difference (95% CRI, credible interval) = 0.02 (-0.31, 0.34)] and LDL [mean difference (95% CRI) = 0.10 (-0.22, 0.43)] when comparing concentrations at the 75th to the 25th percentile. When stratified by BMI, we found suggestive positive relationships between the paraben group and total cholesterol and LDL among women with high BMI [mean difference (95% CRI) = 0.16 (-0.23, 0.55) and 0.16 (-0.25, 0.57)], but not among women with low BMI [mean difference (95% CRI) = -0.02 (-0.16, 0.12) and -0.02 (-0.15, 0.12)]. Among the paraben group, propylparaben was driving the associations among high BMI group [mean difference (95% CRI) = 0.25 (-0.26, 0.75) and 0.35 (-0.25, 0.95)], but not among low BMI group [mean difference (95% CRI) = 0.00 (-0.06, 0.07) and 0.00 (-0.07, 0.07)]. In addition, we found suggestive positive associations of DEHP metabolites and BPA group with total cholesterol, non-HDL and LDL among women with lower BMI, but none of the chemicals was identified as an important contributor to these associations. This study highlights that the associations between EDCs and cardiovascular biomarkers, such as circulating lipids, differ by BMI among pregnant women.
Presentation: 6/1/2024
7784 Perspectives Of Anabolic Steroid Users On Steroid Use, Side Effects, And Patient Education
Authors: Lam David D , Potemkin Michael , Kreutz Jason , Shah Harshil , Patel Parth , Doyle-Baker Patricia K 
Published: October 2024

Abstract
Disclosure: D.D. Lam: None. M. Potemkin: None. J. Kreutz: None. H. Shah: None. P. Patel: None. P.K. Doyle-Baker: None.
Background: Details on first-hand perceived and experienced effects of anabolic steroids (AS) on users are sparse. Furthermore, there is limited information on what type of AS knowledge users possess, irrespective of current published works in AS pharmacology. This study aimed to identify insights of AS users regarding their knowledge and patterns of AS use, commonly experienced side effects, sources used for steroid education, and their unique needs as a population. Study Design: AS users from Calgary, Alberta, Canada of various sexes and ages ≥ 18 were recruited through gyms in Alberta with convenience and snowball sampling techniques. Participant-centered-qualitative research using semi-structured interviews was conducted. Participants discussed their experiences as a user and shared their understanding of AS, details of usage including compounds used, and their commonly experienced or perceived side effects through one-on-one interviews of varying durations. Interviews were coded using NVivo 14 Software prior to thematic analysis. Resulting codes were organized into overarching themes based on researchers' consensus. Results: Seven AS users were confidentially interviewed over Zoom. Several important themes emerged. There existed a wide variety of AS that users have identified, with some of the most commonly mentioned being Anavar, Nolvadex, Testosterone, and Trenbolone. Steroid users frequently identified increased strength with faster recovery from training and better overall appearance as prominent advantages of AS use. Conversely, hair loss, gynecomastia, acne, aggression, erectile dysfunction, altered libido, hypogonadism, infertility, and injection related discomfort were commonly identified disadvantages of AS use. Lastly, individuals using AS report having consulted many different sources to learn about appropriate dosing, techniques, and side effects. These sources ranged from peers, coaches, online forums, social media, and scientific research articles, but rarely included healthcare professionals. Conclusions: This study identified the need for education and research in exploring the impact of AS use. A variety of AS exist on the market, yet information pertaining to the side effect profiles of these AS from a patient’s perspective is lacking and exploration of this topic is warranted. Further investigation as to the impact of user education on their understanding of the risks versus the benefits of AS use must also be conducted.
Presentation: 6/1/2024
6625 Adrenocortical Oncocytoma: Illuminating The Clinical And Biological Behavior Of An Extremely Rare Tumor Of The Adrenal Gland
Authors: Usmani Hareem , Arguello Mingxue 
Published: October 2024

Abstract
Disclosure: H. Usmani: None. M. Arguello, MD: None.
Background: Only 120 cases of adrenocortical oncocytoma have been reported in English literature (1). Standardized diagnostic criteria, or a typical clinical or biological course, have yet to be established. 58 yo woman with PMH HTN, COPD, CREST syndrome on chronic prednisone, previous 38-pk yr smoker, pulmonary nodule, current vaper, was incidentally found to have a left adrenal mass when she went for CT abd pelvis with IV and oral contrast to evaluate for suspected diverticulosis and anemia. CT showed a mass in the left adrenal gland measuring 5.6 x 4.4 cm in size, heterogeneous in density, with possible areas of necrosis. It is unclear how long the adrenal mass was there since the patient never had a CT scan before. Patient was asymptomatic except 50 lb wt loss in the last couple of years with a feeble appetite, self-reported abnormal hair growth (no hirsutism on exam), voice deepening attributed to smoking, and HTN- well controlled with nifedipine. Patient has no FHx of endocrinopathy. Adrenal function tests wnl except for plasma free normetanephrines slightly high at 1.64 nmol/L, which was unlikely from tumor considering tumor size. Normal adrenal function tests included 11-deoxycortisol, DHEA-S, plasma-free metanephrine, aldosterone, renin, aldosterone: renin ratio, androstenedione, ACTH, 1 mg overnight dexamethasone suppression test.She underwent left-sided lap adrenalectomy 4 mos after discovery of adrenal mass, with no complications. 6 days after surgery, a left in JP drain caught on a door at patient’s home, causing splenic subcapsular tear requiring emergent splenectomy. The path report shows an encapsulated neoplasm consisting of sheet-like proliferation of eosinophilia polygonal cells with nuclear atypia, low mitotic activity, and no capsular or vascular invasion. Immunohistochemical markers show neoplastic cells positive for SF1, Mart1, inhibin (focal), and synaptophysin (focal); negative for CK-OSCAR, CK7, CK20, chromogranin, HMB45, and TTF-1, Ki67 and pHH3 show low proliferative index. The pathology slides were sent to U of M, where the diagnosis of adrenal cortical oncocytoma was made. Removal of oncocytoma has not affected patient’s weight, blood pressure, deep voice, or hair growth on 4 month follow up. Lin-Wiess-bisceglia system (2) suggests a benign lesion. But, as mutation rate for this rare tumor is unclear, plan for CT abd pelvis in 1 yr. Conclusion: This case elucidates the clinical and biological course and pathologic diagnosis of an exceedingly rare adrenal tumor. References: [1] Hong Y, Hao Y, Hu J, Xu B, Shan H, Wang X. Adrenocortical oncocytoma: 11 Case reports and review of the literature. Medicine (Baltimore). 2017 Dec;96(48):e8750. [2] Munoz de Nova JL, García-Sanz Í, del Campo Val L, Delgado Valdueza J, Martín-Pérez E. Oncocitoma: una lesión infrecuente en la glándula suprarrenal. Endocrinol Nutr. 2015;62:144-145.
Presentation: 6/1/2024
7088 Serum PFAS Concentrations And Lipid Health In Adolescents : A Cross-Sectional Study From Korean National Environmental Health Survey 2018-2020
Authors: Shin M , Kim S H 
Published: October 2024

Abstract
Disclosure: M. Shin: None. S. Kim: None.
Backgrounds: Perfluoroalkyl substances (PFAS), widespread environmental contaminants, have been implicated in dyslipidemia in adults, but their impact on adolescent lipid profiles remains unclear. This study examines the association between serum PFAS concentrations and subnormal lipid profiles in a representative sample of Korean adolescents. Methods: Utilizing data from the fourth cycle of the Korean National Environmental Health Survey (2018-2020), we conducted a cross-sectional analysis on adolescents aged 12-17 (N=818). Serum PFAS concentrations—specifically PFOS, PFOA, PFHxS, PFNA, and PFDeA—were stratified into tertiles. Adjusted percentage changes in lipid parameters and the odds of suboptimal lipid profiles were calculated using multivariable linear and logistic regression models, adjusting for potential confounders. Results: Higher PFAS concentrations, particularly in the top tertile, were significantly associated with altered lipid profiles. Increased PFOS in the upper tertile corresponded with raised HDL-cholesterol levels, while PFNA and PFDeA showed similar associations with LDL-cholesterol and non-HDL-cholesterol. The logistic regression analysis showed that for PFNA, adolescents in the highest tertile had increased odds of an elevated LDL- cholesterol (OR: 2.24; 95% CI: 1.12-4.48), and PFDeA was associated with an increased total cholesterol (OR: 1.80; 95% CI: 1.21-2.66) when compared to the lowest tertile. Other PFAS compounds did not demonstrate a consistent pattern of significant associations with lipid parameters. Conclusions: These results underscore the possible health consequences of PFAS exposure and the risk of dyslipidemia among adolescents. Additional studies are required to confirm these links and to understand the underlying mechanisms involved.
Presentation: 6/1/2024
8054 Temporal Trends In Thyroidal Endocrine Disruptor Exposure Among United States Reproductive-Age And Pregnant Women Over 20 Years And Differences By Socioeconomic Status
Authors: Han Cheng , Lee Sun , Pearce Elizabeth N 
Published: October 2024

Abstract
Disclosure: C. Han: None. S. Lee: None. E.N. Pearce: None.
Background: Widespread exposure to endocrine disrupting chemicals (EDCs) poses a risk to public health and environmental safety. Women of reproductive age and pregnant women may be uniquely vulnerable to adverse thyroidal effects of EDCs. A better understanding of thyroidal EDCs exposure is needed to inform public health strategies. Objective: To examine temporal trends in EDC exposure and their effects on thyroid function among reproductive-age and pregnant women in the U.S. over the last two decades, with a focus on the impact of socioeconomic status. Methods: We analyzed data from the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2020 in 25,320 reproductive-age women and 2,525 pregnant women to assess the levels of exposure to thyroid-disrupting chemicals, including dioxins, organochlorides, polyfluoroalkyl substances (PFAS) from serum samples, and polyaromatic hydrocarbons, perchlorates, and metabolites from personal care products, phthalates, and plasticizers from urine samples. We used Kruskal-Wallis tests to analyze changes in EDC exposures over the past 20 years and multivariate linear regression models to identify factors influencing these levels. Results: We found a significant decline in exposures to 20 types of dioxins, 7 organochlorides, 6 PFAS, 5 personal care product chemicals, 6 phthalate metabolites, perchlorate, and thiocyanate, but substantial increases in exposure to 2 polyaromatic hydrocarbons among reproductive-age women. Among pregnant women, trends were similar, with declines in exposures to dioxins, organochlorides, PFAS, and phthalate metabolites; whereas exposure to 3 Polyaromatic hydrocarbons, 1 personal care product chemical, nitrates, and thiocyanates increased. Linear regression indicated a significant positive association between socioeconomic status and the levels of dioxin and PFAS in both reproductive-age and pregnant women, and a negative association between socioeconomic status and perchlorate and polyaromatic hydrocarbons in the participants. Conclusion: The past two decades have witnessed a general decline in the levels of most thyroidal EDCs in U.S. reproductive-age and pregnant women; however, some, particularly polyaromatic hydrocarbons, exposures have increased, especially among women with lower socioeconomic status. This has the potential to worsen disparities in health outcomes. These findings highlight the necessity for targeted public health interventions to reduce the impact of EDCs on thyroid health and address socioeconomic health disparities.
Presentation: 6/3/2024
6451 Bone Health ECHO: Expanding Global Capacity To Deliver Best Practice Skeletal Healthcare
Authors: Michael Lewiecki E , Rothman Micol Sara , Bouchonville Matthew Frederick 
Published: October 2024

Abstract
Disclosure: E.M. Lewiecki: None. M.S. Rothman: None. M.F. Bouchonville: None.
1 Bone Health ECHO: Expanding Global Capacity to Deliver Best Practice Skeletal Healthcare E. M. Lewiecki MD,[1] M. S. Rothman MD,[2] M. F. Bouchonville II MD[1] [1]University of New Mexico Health Sciences Center, Albuquerque, NM, USA[2]University of Colorado School of Medicine, Aurora, CO, USA Disclosure: E. M. Lewiecki: Amgen: investigator, consultant, speaker; Radius: investigator, consultant; Ultragenyx: investigator; Kyowa Kirin: consultant, speaker; Angitia: consultant; Ascendis: consultant. M. S. Rothman: nothing to disclose. M. F. Bouchonville II: nothing to disclose. Background: Osteoporosis is a major public health concern worldwide, with one-half of women and one-fifth of men over the age of 50 years having an osteoporotic fracture in their remaining lifetime. Osteoporotic fractures are associated with an increase in morbidity, mortality, and loss of independence. The economic costs of osteoporotic fractures are high and expected to rise along with population aging. Despite the availability of many medications proven to reduce fracture risk, most patients who could benefit from their use are not receiving them. Although fears and misperceptions about medications can exacerbate this treatment gap, an additional pitfall is a shortage of bone health specialists across the US, particularly in rural areas. Given how far many patients have to travel to see a bone health expert, innovative strategies are needed to improve access to care. Introduction: Bone Health ECHO (Extension for Community Healthcare Outcomes) is a virtual community of practice consisting of a network of healthcare professionals who connect online, where all learn and all teach through discussion of patient cases and short didactic presentations. ECHO aims to improve health equity, allowing participants to provide better skeletal healthcare closer to home, at lower cost, with greater convenience than referral to an academic center. Methods: This is a progress report for the prototype Bone Health ECHO program at the University of New Mexico (UNM) Health Sciences Center in Albuquerque, NM, USA, and the development of similar programs worldwide, with data compiled by Project ECHO at UNM. Results: The UNM Bone Health ECHO program has been held weekly since 2015, with a progressive increase in average weekly attendance each year since then. A total of 17 Bone Health ECHO programs have been started, 9 in the United States and 8 in other countries. Each program functions independently while following the ECHO model of case-based learning. Conclusions: The experiences and lessons learned from current Bone Health ECHO programs provide a template for the development of additional programs, with the goal of reducing the burden of skeletal disorders worldwide.
Presentation: 6/2/2024
7746 Developing An Ambulatory Endocrinology, Diabetes And Metabolism Curriculum With On-Site Fellow Involvement
Authors: Mascaro Javier Ocampo , White Caitlin A , Nandiwada Deepa R 
Published: October 2024

Abstract
Disclosure: J. Ocampo Mascaro: None. C.A. White: None. D.R. Nandiwada: None.
Background: Traditionally, Internal Medicine (IM) residency programs have favored inpatient learning opportunities, with limited exposure to outpatient subspecialties like Endocrinology, Diabetes and Metabolism (EDM). Understanding EDM concepts is critical for IM physicians. Over the last decade, programs have been able to increase outpatient component in their curriculum. Thoughtful educational interventions that bolster exposure to EDM topics for IM residents are necessary to both reduce gaps in medical knowledge and address dwindling numbers of applicants interested in pursuing a career in EDM. On-site EDM fellow participation presents a unique opportunity to achieve these goals. Methods: An electronic needs assessment was delivered to Primary Care (PC) IM residents at a large academic medical center. The survey included questions gauging their degree of confidence in diagnosing, managing, and counseling patients with frequent EDM concerns. All questions were answered using a Likert scale. Data was collected from August to October 2023 and used to design an educational intervention by a senior EDM fellow who is physically present at the primary care IM clinic monthly. This intervention encompassed didactic lectures, direct observation of patient counseling, and on-site curbside consultation. Results: 12 of 24 PC IM residents completed the survey. Most felt somewhat or extremely comfortable evaluating patients with diabetes mellitus (DM) type 2 (100%) and hypothyroidism (83%). When evaluating adrenal insufficiency, osteoporosis, adrenal incidentalomas and primary aldosteronism (PA), 91%, 67%, 67% and 67% of residents reported being extremely or somewhat uncomfortable, respectively. Residents reported being somewhat or extremely comfortable addressing lifestyle interventions for weight loss (83%), medications for DM2 (83%), and instructing patients how to take levothyroxine (67%). Residents felt most uncomfortable counseling patients about osteoporosis treatment, adrenal incidentaloma, osteoporosis screening, fall prevention, and insulin teaching, with 83%, 67%, 58%, 58%, and 58% of residents reporting being somewhat or extremely uncomfortable, respectively. Conclusion: This needs assessment highlighted PC IM residents at our institution were uncomfortable evaluating adrenal insufficiency, osteoporosis, adrenal incidentalomas, and PA. They were also uncomfortable counseling patients on these topics as well as fall prevention and insulin teaching. We describe an intervention by an on-site EDM fellow utilizing pre-clinic conferences, direct observation, and on-site curbside consultations. As IM training pathways have increased exposure to outpatient subspecialties, it is important to thoughtfully assess and design interventions to maximize trainees' learning potential, which can be accomplished with subspecialty fellow direction.
Presentation: 6/3/2024