bluuebunny/crossref_metadata_2025_split huggingface dataset:
FAULT TOLERANT CONTROL FOR SENSOR FAULT OF A SINGLE-LINK FLEXIBLE MANIPULATOR SYSTEM
Authors: Abd Latip Siti Fadilah , Husain Abdul Rashid , Ahmad Mohamad Noh , Mohamed Zaharuddin 
Published: June 2016

This paper presents a new approach for sensor fault tolerant control (FTC) of a single-link flexible manipulator system (FMS) by using Finite Element Method (FEM). In this FTC scheme, a new control law is proposed where it is added to the nominal control. This research focuses on one element without any payload assumption in the modelling. The FTC method is designed in such way that aims to reduce fault while maintaining nominal FMS controller without any changes in both faulty and fault free cases. This proposed FTC approach is achieved by augmenting Luenberger observer that is capable of estimating faults in fault detection and isolation (FDI) analysis. From the information provided by the FDI, fault magnitude is assessed by using Singular Value Decomposition (SVD) where this information is used in the fault compensation strategy. For the nominal FMS controller, Proportional- integral- derivative (PID) controller is used to control the FMS where it follows the desired hub angle. This work proved that the FTC approach is capable of reducing fault with both incipient and abrupt signals and in two types of faulty conditions where the sensor is having loss of effectiveness and totally malfunction. All the performances are compared with FTC with Unknown Input Observer (FTC-UIO) method via the integral of the absolute magnitude of error (IAE) method.
Parasympathetic innervation of vertebrobasilar arteries: is this a potential clinical target?
Authors: Roloff Eva v. L. , Tomiak‐Baquero Ana M. , Kasparov Sergey , Paton Julian F. R. 
Published: June 2016

AbstractThis review aims to summarise the contemporary evidence for the presence and function of the parasympathetic innervation of the cerebral circulation with emphasis on the vertebral and basilar arteries (the posterior cerebral circulation). We consider whether the parasympathetic innervation of blood vessels could be used as a means to increase cerebral blood flow. This may have clinical implications for pathologies associated with cerebral hypoperfusion such as stroke, dementia and hypertension. Relative to the anterior cerebral circulation little is known of the origins and neurochemical phenotypes of the parasympathetic innervation of the vertebrobasilar arteries. These vessels normally provide blood flow to the brainstem and cerebellum but can, via the Circle of Willis upon stenosis of the internal carotid arteries, supply blood to the anterior cerebral circulation too. We review the multiple types of parasympathetic fibres and their distinct transmitter mechanisms and how these vary with age, disease and species. We highlight the importance of parasympathetic fibres for mediating the vasodilatory response to sympathetic activation. Current trials are investigating the possibility of electrically stimulating the postganglionic parasympathetic ganglia to improve cerebal blood flow to reduce the penumbra following stroke. We conclude that although there are substantial gaps in our understanding of the origins of parasympathetic innervation of the vertebrobasilar arteries, activation of this system under some conditions might bring therapeutic benefits.
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Use of Multiple Sequencing Technologies To Produce a High-Quality Genome of the Fungus
Pseudogymnoascus destructans
, the Causative Agent of Bat White-Nose Syndrome
Authors: Drees Kevin P. , Palmer Jonathan M. , Sebra Robert , Lorch Jeffrey M. , Chen Cynthia , Wu Cheng-Cang , Bok Jin Woo , Keller Nancy P. , Blehert David S. , Cuomo Christina A. , Lindner Daniel L. , Foster Jeffrey T. 
Published: July 2016

ABSTRACT

White-nose syndrome has recently emerged as one of the most devastating wildlife diseases recorded, causing widespread mortality in numerous bat species throughout eastern North America. Here, we present an improved reference genome of the fungal pathogen
Pseudogymnoascus destructans
for use in comparative genomic studies.
Genome Sequence of
Gordonia
Bacteriophage Lucky10
Authors: Pope Welkin H. , Brown Aleks K. , Fisher Daniel J. , Okwiya Nicholas H. , Savage Kaitlyn A. , German Brian A. , McDonnell Jill E. , Schafer Claire E. , Yu Victor J. , Furbee Emily C. , Grubb Sarah R. , Warner Marcie H. , Montgomery Matthew T. , Garlena Rebecca A. , Russell Daniel A. , Jacobs-Sera Deborah , Hatfull Graham F. 
Published: July 2016

ABSTRACT

Lucky10 is a newly isolated phage of
Gordonia terrae
3612 that was recovered from a soil sample in Pittsburgh, PA. Lucky10 has siphoviral morphology and a double-stranded DNA (dsDNA) genome of 42,979 bp, with 70 predicted protein-coding genes. Lucky10 shows little similarity to previously reported
Gordonia
phages.
Genome Sequences of
Gordonia
Bacteriophages Obliviate, UmaThurman, and Guacamole
Authors: Pope Welkin H. , Akbar Armaan F. , Ayers Taylor N. , Belohoubek Selena G. , Chung Connie F. , Hartman Allison C. , Kayiti Tejus , Kessler Cecilia M. , Koman Philipp I. , Kotovskiy Grigoriy A. , Morgan Taylor M. , Rohac Rebecca M. , Silva Gabriela M. , Willis Charles E. , Milliken Katherine A. , Shedlock Kathleen A. , Stanton Ann-Catherine J. , Toner Chelsea L. , Furbee Emily C. , Grubb Sarah R. , Warner Marcie H. , Montgomery Matthew T. , Garlena Rebecca A. , Russell Daniel A. , Jacobs-Sera Deborah , Hatfull Graham F. 
Published: July 2016

ABSTRACT

We describe three newly isolated phages—Obliviate, UmaThurman, and Guacamole—that infect
Gordonia terrae
3612. The three genomes are related to one another but are not closely related to other previously sequenced phages or prophages. The three phages are predicted to use integration-dependent immunity systems as described in several mycobacteriophages.
Genome Sequence of Bradyrhizobium stylosanthis Strain BR 446
T
, a Nitrogen-Fixing Symbiont of the Legume Pasture
Stylosanthes guianensis
Authors: Delamuta Jakeline Renata Marçon , Ribeiro Renan Augusto , Gomes Douglas Fabiano , Souza Renata Carolini , Chueire Ligia Maria Oliveira , Hungria Mariangela 
Published: July 2016

ABSTRACT

Bradyrhizobium stylosanthis
BR 446
T
is a nitrogen-fixing symbiont of the tropical legume pasture
Stylosanthes guianensis
. Its draft genome contains 8,801,717 bp and 8,239 coding sequences (CDSs). Several putative genes that might confer high competitiveness and saprophytic capacity under the stressful conditions of tropical soils were identified in the genome.
In Vivo
Examination of Mouse APOBEC3- and Human APOBEC3A- and APOBEC3G-Mediated Restriction of Parvovirus and Herpesvirus Infection in Mouse Models
Authors: Nakaya Yuki , Stavrou Spyridon , Blouch Kristin , Tattersall Peter , Ross Susan R. 
Published: June 2016

ABSTRACT

APOBEC3 knockout and human APOBEC3A and -3G transgenic mice were tested for their ability to be infected by the herpesviruses herpes simplex virus 1 and murine herpesvirus 68 and the parvovirus minute virus of mice (MVM). Knockout, APOBEC3A and APOBEC3G transgenic, and wild-type mice were equally infected by the herpesviruses, while APOBEC3A but not mouse APOBEC3 conferred resistance to MVM. No viruses showed evidence of cytidine deamination by mouse or human APOBEC3s. These data suggest that
in vitro
studies implicating APOBEC3 proteins in virus resistance may not reflect their role
in vivo
.

IMPORTANCE
It is well established that APOBEC3 proteins in different species are a critical component of the host antiretroviral defense. Whether these proteins also function to inhibit other viruses is not clear. There have been a number of
in vitro
studies suggesting that different APOBEC3 proteins restrict herpesviruses and parvoviruses, among others, but whether they also work
in vivo
has not been demonstrated. Our studies looking at the role of mouse and human APOBEC3 proteins in transgenic and knockout mouse models of viral infection suggest that these restriction factors are not broadly antiviral and demonstrate the importance of testing their activity
in vivo
.
Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge
Authors: Kim Seong K. , Shakya Akhalesh K. , O'Callaghan Dennis J. 
Published: June 2016

ABSTRACT
Equine herpesvirus 1 (EHV-1) is a major pathogen affecting equines worldwide. The virus causes respiratory disease, abortion, and, in some cases, neurological disease. EHV-1 strain KyA is attenuated in the mouse and equine, whereas wild-type strain RacL11 induces severe inflammation of the lung, causing infected mice to succumb at 4 to 6 days postinfection. Our previous results showed that KyA immunization protected CBA mice from pathogenic RacL11 challenge at 2 and 4 weeks postimmunization and that KyA infection elicited protective humoral and cell-mediated immune responses. To investigate the protective mechanisms of innate immune responses to KyA, KyA-immunized mice were challenged with RacL11 at various times postvaccination. KyA immunization protected mice from RacL11 challenge at 1 to 7 days postimmunization. Immunized mice lost less than 10% of their body weight and rapidly regained weight. Virus titers in the lungs of KyA-immunized mice were 1,000-fold lower at 2 days post-RacL11 challenge than virus titers in the lungs of nonimmunized mice, indicating accelerated virus clearance. Affymetrix microarray analysis revealed that gamma interferon (IFN-γ) and 16 antiviral interferon-stimulated genes (ISGs) were upregulated 3.1- to 48.2-fold at 8 h postchallenge in the lungs of RacL11-challenged mice that had been immunized with KyA. Murine IFN-γ inhibited EHV-1 infection of murine alveolar macrophages and protected mice against lethal EHV-1 challenge, suggesting that IFN-γ expression is important in mediating the protection elicited by KyA immunization. These results suggest that EHV-1 KyA may be used as a live attenuated EHV-1 vaccine as well as a prophylactic agent in horses.

IMPORTANCE
Viral infection of cells initiates a signal cascade of events that ultimately attempts to limit viral replication and prevent infection through the expression of host antiviral proteins. In this study, we show that EHV-1 KyA immunization effectively protected CBA mice from pathogenic RacL11 challenge at 1 to 7 days postvaccination and increased the expression of IFN-γ and 16 antiviral interferon-stimulated genes (ISGs). The administration of IFN-γ blocked EHV-1 replication in murine alveolar macrophages and mouse lungs and protected mice from lethal challenge. To our knowledge, this is the first report of an attenuated EHV-1 vaccine that protects the animal at 1 to 7 days postimmunization by innate immune responses. Our findings suggested that IFN-γ serves as a novel prophylactic agent and may offer new strategies for the development of anti-EHV-1 agents in the equine.
Viral FGARAT Homolog ORF75 of Rhesus Monkey Rhadinovirus Effects Proteasomal Degradation of the ND10 Components SP100 and PML
Authors: Hahn Alexander S. , Großkopf Anna K. , Jungnickl Doris , Scholz Brigitte , Ensser Armin 
Published: June 2016

ABSTRACT

Nuclear domain 10 (ND10) components restrict herpesviral infection, and herpesviruses antagonize this restriction by a variety of strategies, including degradation or relocalization of ND10 proteins. The rhesus monkey rhadinovirus (RRV) shares many key biological features with the closely related Kaposi's sarcoma-associated herpesvirus (KSHV; human herpesvirus 8) and readily infects cells of both human and rhesus monkey origin. We used the clustered regularly interspaced short palindromic repeat-Cas9 (CRISPR-Cas9) technique to generate knockout (ko) cells for each of the four ND10 components, PML, SP100, DAXX, and ATRX. These ko cells were analyzed with regard to permissiveness for RRV infection. In addition, we analyzed the fate of the individual ND10 components in infected cells by immunofluorescence and Western blotting. Knockout of the ND10 component DAXX markedly increased RRV infection, while knockout of PML or SP100 had a less pronounced effect. In line with these observations, RRV infection resulted in rapid degradation of SP100, followed by degradation of PML and the loss of ND10 structures, whereas the protein levels of ATRX and DAXX remained constant. Notably, inhibition of the proteasome but not inhibition of
de novo
gene expression prevented the loss of SP100 and PML in cells that did not support lytic replication, compatible with proteasomal degradation of these ND10 components through the action of a viral tegument protein. Expression of the RRV FGARAT homolog ORF75 was sufficient to effect the loss of SP100 and PML in transfected or transduced cells, implicating ORF75 as the viral effector protein.

IMPORTANCE
Our findings highlight the antiviral role of ND10 and its individual components and further establish the viral FGARAT homologs of the gammaherpesviruses to be important viral effectors that counteract ND10-instituted intrinsic immunity. Surprisingly, even closely related viruses like KSHV and RRV evolved to use different strategies to evade ND10-mediated restriction. RRV first targets SP100 for degradation and then targets PML with a delayed kinetic, a strategy which clearly differs from that of other gammaherpesviruses. Despite efficient degradation of these two major ND10 components, RRV is still restricted by DAXX, another abundant ND10 component, as evidenced by a marked increase in RRV infection and replication upon knockout of DAXX. Taken together, our findings substantiate PML, SP100, and DAXX as key antiviral proteins, in that the first two are targeted for degradation by RRV and the last one still potently restricts replication of RRV.
Duration and nature of the end-Cryogenian (Marinoan) glaciation
Authors: Prave Anthony R. , Condon Daniel J. , Hoffmann Karl Heinz , Tapster Simon , Fallick Anthony E. 
Published: June 2016

Abstract
The end-Cryogenian glaciation (Marinoan) is portrayed commonly as the archetype of snowball Earth, yet its duration and character remain uncertain. Here we report U-Pb zircon ages for two ash beds from widely separated localities of the Marinoan-equivalent Ghaub Formation in Namibia: 639.29 ± 0.26 Ma and 635.21 ± 0.59 Ma. These findings verify, for the first time, the key prediction of the snowball Earth hypothesis for the Marinoan glaciation, i.e., longevity, with a duration of ≥4 m.y. They also show that the nonglacial interlude of Cryogenian time spanned 20 m.y. or less and that glacigenic erosion and sedimentation, and at least intermittent open-water conditions, occurred 4 m.y. prior to termination of the Marinoan glaciation.
Red eyes and red-flags: improving ophthalmic assessment and referral in primary care
Authors: Kilduff Caroline , Lois Charis 
Published: June 2016

Up to five percent of primary care consultations are eye-related, yet 96% of General Practitioners (GPs) do not undergo postgraduate ophthalmology training. Most do not feel assured performing eye assessments. Some red eye conditions can become sight threatening, and often exhibit red-flag features. These features include moderate pain, photophobia, reduced visual acuity (VA), eye-trauma, or unilateral marked redness. The aim of this project was to improve primary care assessment and referral of patients presenting with red-flag features based on the NICE ‘Red Eye’ Clinical Knowledge Summary recommendations.Data was collected retrospectively from 139 red eye consultations. A practice meeting highlighted poor awareness of red-flag features, low confidence levels in eye assessments, and time-constraints during appointments. Interventions were based on feedback from staff. These included a primary care teaching session on red-flag features, a VA measurement tutorial, and provision of a red eye toolkit, including VA equipment, to each consultation room.At baseline, each patient had on average 0.9 red-flag features assessed. Only 36.0% (9/25) of patients with red-flag features were appropriately referred to same-day ophthalmology services. Following two improvement cycles, a significant improvement was seen in almost every parameter. On average, each patient had 2.7 red-flag features assessed (vs 0.9, p<0.001). VA was assessed in 55.6% of consultations (vs 7.9%, p<0.001), pain was quantified in 81.5% (vs 20.9%, p=0.005), eye-trauma or foreign-body (51.8% vs 8.6%, p<0.001), extent of redness was documented in 66.7% (vs 14.4%, p<0.001). Only photophobia remained poorly assessed (18.5% vs 14.4%, p=0.75). Following this, 75.0% (6/8) of patients were appropriately referred.This project reflected the literature regarding low confidence and inexperience amongst GPs when faced with ophthalmic conditions. Improvements in education are required to ensure accurate assessments can be undertaken in a time-constrained environment.
Evaluation of canola meal derived from Brassica juncea and Brassica napus on rumen fermentation and nutrient digestibility by feedlot heifers fed finishing diets
Authors: Nair J. , Penner G.B. , Yu P. , (Bart) Lardner H.A. , McAllister T.A. , Damiran D. , McKinnon J.J. 
Published: June 2016

Five yearling heifers (387 ± 6.5 kg) were utilized in a 5 × 5 Latin square to evaluate the substitution of canola meal (CM) derived from Brassica (B.) napus and B.juncea seed for barley grain on feed intake and nutrient utilization. The control diet (CONTROL) consisted of 88.3% barley grain, 4.4% barley silage, and 7.3% supplement [dry matter (DM) basis]. The four CM treatments included 10% and 20% B.napus or B.juncea meal, with CM replacing barley grain. Dry matter intake was not affected (P > 0.05) by treatment. Total duration and area under rumen pH 5.8, 5.5, and 5.2 increased (P < 0.05) with greater inclusion of CM, regardless of type. Cattle fed 20% CM had elevated rumen ammonia-N concentration and urinary N excretion. Cattle fed B. juncea meal exhibited greater (P < 0.05) apparent nutrient digestibility compared with those fed B. napus meal. In conclusion, meal derived from B. napus or B. juncea can be used to replace barley grain at levels up to 20% (DM) in finishing diets without compromising rumen fermentation or total tract nutrient digestibility. However, animal performance and possible economic benefits need to be balanced against the increasing N excretion resulting from over-feeding protein to cattle.
Rebound Effects Caused by Withdrawal of MET Kinase Inhibitor Are Quenched by a MET Therapeutic Antibody
Authors: Pupo Emanuela , Ducano Nadia , Lupo Barbara , Vigna Elisa , Avanzato Daniele , Perera Timothy , Trusolino Livio , Lanzetti Letizia , Comoglio Paolo M. 
Published: July 2016

Abstract
MET oncogene amplification is emerging as a major mechanism of acquired resistance to EGFR-directed therapy in lung and colorectal cancers. Furthermore, MET amplification predicts responsiveness to MET inhibitors currently in clinical trials. Among the anti-MET drugs available, ATP-competitive small-molecule kinase inhibitors abrogate receptor autophosphorylation and downstream activation of ERK1/2 and AKT, resulting in cell-cycle arrest. However, this antiproliferative effect allows persistence of a pool of cancer cells that are quiescent but alive. Once the inhibition is removed, rebound activation of MET-driven cell proliferative pathways and tumor growth may occur, an adverse event observed frequently in clinical settings after drug discontinuation. Here we show that inhibitor withdrawal prompts receptor phosphorylation to levels higher than those displayed at steady-state and generates a rebound effect pushing quiescent cancer cells back into the cell cycle, both in vitro and in experimental tumor models in vivo. Mechanistically, we found that inhibitor treatment blocks MET endocytosis, causing a local increase in the number of receptors at the plasma membrane. Upon inhibitor washout, the receptor is readily rephosphorylated. The initial phosphorylation is not only increased but also prolonged in duration due to downmodulation of a phosphatase-mediated MET-negative feedback loop, which accompanies receptor internalization. Notably, treatment with a MET therapeutic antibody that induces proteolytic cleavage of the receptor at the cell surface substantially prevents this rebound effect, providing a rationale to combine or alternate these mechanistically different types of MET-targeted therapy. Cancer Res; 76(17); 5019–29. ©2016 AACR.
Imaging of Esophageal Lymph Node Metastases by Desorption Electrospray Ionization Mass Spectrometry
Authors: Abbassi-Ghadi Nima , Golf Ottmar , Kumar Sacheen , Antonowicz Stefan , McKenzie James S. , Huang Juzheng , Strittmatter Nicole , Kudo Hiromi , Jones Emrys A. , Veselkov Kirill , Goldin Robert , Takats Zoltan , Hanna George B. 
Published: July 2016

Abstract
Histopathological assessment of lymph node metastases (LNM) depends on subjective analysis of cellular morphology with inter-/intraobserver variability. In this study, LNM from esophageal adenocarcinoma was objectively detected using desorption electrospray ionization-mass spectrometry imaging (DESI-MSI). Ninety lymph nodes (LN) and their primary tumor biopsies from 11 esophago-gastrectomy specimens were examined and analyzed by DESI-MSI. Images from mass spectrometry and corresponding histology were coregistered and analyzed using multivariate statistical tools. The MSIs revealed consistent lipidomic profiles of individual tissue types found within LNs. Spatial mapping of the profiles showed identical distribution patterns as per the tissue types in matched IHC images. Lipidomic profile comparisons of LNM versus the primary tumor revealed a close association in contrast to benign LN tissue types. This similarity was used for the objective prediction of LNM in mass spectrometry images utilizing the average lipidomic profile of esophageal adenocarcinoma. The multivariate statistical algorithm developed for LNM identification demonstrated a sensitivity, specificity, positive predictive value, and negative predictive value of 89.5%, 100%, 100%, and 97.2%, respectively, when compared with gold-standard IHC. DESI-MSI has the potential to be a diagnostic tool for perioperative identification of LNM and compares favorably with techniques currently used by histopathology experts. Cancer Res; 76(19); 5647–56. ©2016 AACR.