bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and expression profiling of MST, SUT and SWEET transporters in Dendrobium catenatum
Authors: Fan Weiwei , Gao Huashan , Zhang Lei , Mao Dongdong , Li Yanyan , Zhang Lifang , Li Jingting , Zhao Xuyao , Hou Hongwei 
Published: September 2024

Abstract

Background
Sugar transporters (STs) play a critical part in the transporting of sugar, be involved in plant growth and development, stress/defense and signal transduction. Dendrobium catenatum (also name as Dendrobium officinale, here after D. catenatum) is an important traditional Chinese medicinal herb with remarkable medicinal functions and high economic value. Polysaccharides are the primary active components exerting various pharmacological activities in D. catenatum. Sugar transporters function as material supplier and may play the essential roles in the polysaccharide biosynthesis, as well as the key player in the abiotic stress signaling and responses in Dendrobium plants. However, there is still a lack of comprehensive analysis of sugar transporters in D. catenatum, and limits the understanding of sugar partitioning in D. catenatum.
Results
In this work, we identified the members of MST, SUT, and SWEET gene families in four Dendrobium plants. Phylogenetic analysis, structural arrangement, regulatory networks, expression profiles, and potential functions of sugar transporters were systematic study in D. catenatum. Seven DcSTs involved in polysaccharide biosynthesis was identified using WGCNA. Red-blue light is an effective way to improve the accumulation of polysaccharides, and influences the expression of polysaccharide biosynthesis related genes.
Conclusions
This study provide insight into the evolution and functional annotation of sugar transporters in Dendrobium, and lays a foundation for future functional research of sugar transporters involved in polysaccharide biosynthesis and stress response.
Co-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using Bac-to-Bac baculovirus expression system
Authors: Bdzhola Anna , Malanchuk Oksana , Palchevskyi Sergii , Gout Ivan , Filonenko Valeriy , Zhyvoloup Alexander 
Published: September 2024

Abstract

Ribosomal protein S6 kinase 1 (p70S6K1) is a member of the AGC family of serine/threonine kinases and is implicated in a diverse range of cellular processes, including protein synthesis, cell growth, and survival. Dysregulation of p70S6K1, characterized by its overexpression and/or overactivation, has been widely implicated in various human pathologies, particularly in several types of cancer. Thus, the generation of active and recombinant p70S6K1 is critical for investigating its role in cancer biology and for developing novel diagnostic or therapeutic approaches. Here, we report a reliable and efficient methodology for the expression and purification of highly active p70S6K1 (His-actS6K1) in quantity and quality that is suitable for biochemical studies and high-throughput enzymatic assays. To achieve this, we utilized the baculovirus dual expression system, which enabled the co-expression of two recombinant proteins in infected cells: a) His-tagged S6K1 with a deletion of the C-terminal autoinhibitory motif and a phosphomimetic mutation at the mTORC1 phosphorylation site (T389D); and b) untagged PDPK1 lacking the PH domain. Efficient expression of both recombinant proteins was achieved, resulting in highly pure preparations of His-actS6K1. The high activity of the purified kinase was confirmed by various kinase assays, demonstrating significantly higher levels of substrate phosphorylation compared to the tested commercial product. Overall, our developed methodology offers a rapid and cost-effective approach for producing constitutively active His-actS6K1, which can be utilized in academic research and biotechnology.
δ-α cell-to-cell interactions modulate pancreatic islet Ca2+ oscillation modes
Authors: Tang Chao , Ren Huixia , Li Yanjun , Xie Beichen , Qian Weiran , Yu Yi , Chang Tianyi , Yang Xiaojing , Sneppen Kim , Chen Liangyi 
Published: September 2024

Abstract

Glucose-induced pancreatic islet hormone release is tightly coupled with oscillations in cytoplasmic free Ca2+ concentration of islet cells, which is regulated by a complex interplay between intercellular and intracellular signaling. δ cells, which entangle with α cells located at the islet periphery, are known to be important paracrine regulators. However, the role of δ cells in regulating Ca2+ oscillation pattern remains unclear. Here we show that δ-α cell-to-cell interactions are the source of variability in glucose-induced Ca2+ oscillation pattern. Somatostatin secreted from δ cells prolonged the islet’s oscillation period in an α cell mass-dependent manner. Pharmacological and optogenetic perturbations of δ-α interactions led islets to switch between fast and slow Ca2+ oscillations. Continuous adjustment of δ-α coupling strength caused the fast oscillating islets to transition to mixed and slow oscillations. We developed a mathematical model, demonstrating that the fast-mixed-slow oscillation transition is a Hopf bifurcation. Our findings provide a comprehensive understanding of how δ cells modulate islet Ca2+ dynamics and reveal the intrinsic heterogeneity of islets due to the structural composition of different cell types.
Laboratory and field assays indicate that a widespread no-see-um, Culicoides furens Poey is susceptible to permethrin
Authors: Cooper Vilma M. , Buckner Eva A. , Jiang Yongxing , Burkett-Cadena Nathan 
Published: September 2024

Abstract

The recent reemergence of Oropouche virus (OROV) highlights the need to better understand insecticide susceptibility in Culicoides (Diptera: Ceratopogonidae), which contains the vector of OROV and many other species that are biting nuisances and vectors of pathogens that affect humans, livestock, and wildlife. With adulticides as the primary method of Culicoides control, there is growing concern about insecticide resistance, compounded by the lack of tools to monitor Culicoides susceptibility. We adapted the CDC bottle bioassay and field cage trial methods, typically used to monitor insecticide susceptibility in mosquitoes and formulated adulticide efficacy, to evaluate permethrin susceptibility in the widely distributed coastal nuisance species, Culicoides furens. Permethrin caused complete mortality in C. furens in field and laboratory assays. We identified a diagnostic dose (10.75 µg) and time (30 minutes) that resulted in complete mortality in CDC bottle bioassays. Additionally, we determined that no-see-um netting is an effective mesh for field cage trials, allowing for accurate assessment of Culicoides susceptibility to ultra-low volume applications of formulated adulticides like Permanone 30–30, a widely utilized adulticide. These methodologies offer essential tools for assessing Culicoides susceptibility, which is crucial for managing populations of Culicoides and preventing the spread of OROV and other pathogens.
Navigating the black box of fair national emissions targets
Authors: Dekker Mark , Hof Andries , du Pont Yann Robiou , Berg Nicole van den , Daioglou Vassilis , Elzen Michel Den , Heerden Rik van , Hooijschuur Elena , Tagomori Isabela S. , Würschinger Chantal , Vuuren Detlef van 
Published: September 2024

Abstract

Current national emissions targets fall short of meeting the Paris Agreement climate goals, prompting increased attention on equitable ways to closing this gap in international climate negotiations. Fair emissions allocations are often determined using effort-sharing formulas based on principles of fairness, yielding diverse outcomes. This diversity in equity-based emissions allocations complicates efforts by policymakers and courts to discern the effects of assumptions and uncertainties from normative decisions. Here, we provide up-to-date fair emissions allocations in a comprehensive framework, accounting for considerations across three dimensions: physical and social uncertainties, global strategies and equity. The relative impact of these considerations is assessed using Sobol’s method, quantifying for which countries discussions on equity and socio-economic projections are important, and for whom global targets dominate. The fair emissions allocations are compared to NDCs and cost-optimal scenario output, identifying countries that have insufficient ambition and those for which – from a globally cost-optimal perspective – it is appealing to invest in mitigation abroad.
Psilocybin ameliorates neuropathic pain-like behaviour in mice and facilitates the gabapentin-mediated analgesia
Authors: Askey Tatum , Allen-Ross Daniel , Lasrado Reena , Gilmour Gary , Hunt Stephen , Tamagnini Francesco , Ahmed Maqsood , Stephens Gary , Maiarú Maria 
Published: September 2024

Abstract

Chronic pain states are challenging to control with current drug therapies. Here, we demonstrate that a single dose of psilocybin can produce a sustained anti-nociceptive effect in a mouse model of chronic neuropathic pain. Beyond this, the single dose of psilocybin caused a dramatic increase in the anti-nociceptive potential of gabapentin, a widely used treatment for neuropathic pain, such data are suggestive of establishment of longer lasting changes in network processing.
Flexible budgetary process and institutional performance of University of Environment and Sustainable Development
Authors: Nyanful Steve Essilfie 
Published: September 2024

Abstract

This research paper examines the correlation between the University of Environment and Sustainable Development's Budgetary Process and Performance. Utilizing a descriptive research design, the study collected primary data from a targeted sample of 167 functional leaders involved in the budgeting process at the university, out of 289 staff members. The study found that decentralizing financial management to university departments resulted in inefficiencies related to technology adoption, control measures, and the timeliness of supplemental budgeting, alongside notable weaknesses in internal controls due to inconsistent budget consultations. In contrast, the budget evaluation process was deemed efficient, evidenced by the budget committee's receipt of accurate and timely financial data on a quarterly basis.
Model-based analysis of the impact of climate change on hydrology in the Guayas River basin (Ecuador)
Authors: Ghafoor Jawad , Forio Marie Anne Eurie , Nolivos Indira , Arias-Hidalgo Mijial , Goethals Peter L. M. 
Published: September 2024

ABSTRACT
Worldwide climate change will most likely lead to drastic changes in hydrology and food production. In this study, the impact of climate change on the hydrological regime and the fate of pesticides in the Guayas River basin is investigated using the Soil and Water Assessment Tool. Four general circulation models and three representative concentration pathways (RCP 4.5, RCP 6.0 and RCP 8.5) for three future periods were used to assess impact of climate change. Future projections showed a maximum increase in the average monthly precipitation of 40% in June, as well as an increase in an average minimum temperature of 3.85°C for July and an average maximum temperature of 4.5°C for August in 2080s. The model simulations based on RCP 8.5 scenario predict an increase in potential evapotranspiration by 11%, surface runoff of 39% and water yield of 33% in 2080s. The pesticide simulation showed the highest water concentrations during the wet season. Projections of trends in pesticide concentration indicate a similar trend to the current situation given the application rate remains the same. The results can be beneficial for the management and planning of the basin to mitigate flood and water quality-related impacts of food production and climate change.
Ecosystem service change from lost surface water for farming in the Middle Drâa Valley, southern Morocco: an economic valuation through a replacement cost approach
Authors: Mahjoubi Imane , Frör Oliver 
Published: September 2024

ABSTRACT

Water scarcity in the Middle Drâa Valley threatens self-sufficient farming, impacts agricultural production and livelihoods of the oases’ population. To compensate for the lost surface-water resources, farmers increasingly access groundwater resources for irrigation. In this paper, we test the replacement cost approach (RCA) to estimate the monetary value of irrigation water and the minimum amount of ecosystem services’ value lost in the past. A cost-based survey of 107 randomly selected farms was conducted in 2022 to assess the costs of technical substitutes farmers used to replace reduced surface water over the past 20 years. We assess and contrast the average costs across each entire oasis and at the level of each farm. Results show that the losses incurred from the loss of surface water did not follow the aridity gradient, and these losses varied due to water regulation practices, investment capacity, other income-generating activities, and others. Results suggest that the replacement is cheap per unit of metres dug, hectares, and kg of dates, providing an advantage in terms of economy of scale for large farms. The analysis provides insights into the challenges faced by small-scale farmers in accessing water and can contribute to forecasting farmers’ behavior under water scarcity.
Latest Findings on the Effects of Gold Nanoparticles on the Storage Quality of Blood Products (2011-2022) - A Narrative Review
Authors: Zadeh Mehrizi Tahereh , Rezayat Seyed Mahdi , Ebrahimi Shahmabadi Hasan 
Published: September 2024

Abstract:
A wide range of challenges are faced during the storage of blood products, including
storage lesions, contamination that must be removed, and cell and protein damage due to chemicals
and UV exposure. The enhancement of stability exhibited by gold nanoparticles (GNPs) is a
notable advantage of these nanoparticles for the storage of blood products. The results of our review
of articles from 2011 to 2022 discussing the effect of GNPs on blood products revealed that
in RBCs, the dose, concentration, amount, and surface charge of GNPs significantly affect their
compatibility. Purified GNPs were compatible with RBCs. Negatively charged GNPs with smaller
diameters at lower concentrations were more compatible. However, in the plasma product, the
nanoparticle surface modification with different agents showed greater compatibility. PEGylated
nanospheres and GNPs exhibited higher albumin conformational stability than those coated with
cetyltrimethylammonium bromide and rods. In the platelet product, smaller GNPs and high GNP
concentrations induce platelet aggregation. PEGylation increased the platelet compatibility of
GNP. The combination of GNPs with human fibrinogen and clopidogrel prevented clot formation.
Finally, the findings of this investigation demonstrate that GNPs are contingent on their surface
charge, dosage, and concentration.
A Complete Appraisal of Polymeric Micelle-Based Nanocarrier for Targeting Breast Cancer: Recent Strategies and Advancements
Authors: Ray Supriya , Singh Dilpreet 
Published: September 2024

Aim/Objective:
Polymeric micelle-based drug delivery systems have emerged as a beacon
of hope in the challenging landscape of breast cancer therapy. This review aimed to explore the recent
advancements and promising developments in the field, offering an in-depth analysis of their potential
and challenges. The significance of this study lies in providing a comprehensive examination of the
current advances in polymeric micelles technology, which have the potential to improve the efficiency
and specificity of breast cancer therapy while minimizing adverse events.

Methods:
Polymeric micelles offer a revolutionary therapy by encapsulating therapeutic agents, allowing
for controlled drug release and the precise targeting of malignant cells, while lowering systemic
toxicity. Recent advances have focused on enhancing targeting strategies, including active and
passive targeting, to increase the specificity of polymeric micelle-based therapies. A systematic review
of literature was carried out using databases, like PubMed, Scopus, and Web of Science, which
included papers published between 2010 and 2023. The selection criteria of the literature focused on
the drug-loading capabilities and targeting strategies of the polymeric micelles, which could be advantageous
for breast cancer treatment.

Results:
Clinical drug approaches leverage the versatility of micelles to co-deliver multiple agents,
addressing the multifaceted nature of breast tumors and combating drug resistance. Additionally, polymeric
micelles are finding applications in breast cancer diagnostics and imaging, enabling early
detection and precise monitoring. The journey of polymeric micelle-based breast cancer therapy extends
to formulation and engineering, preclinical and clinical studies, and confronts regulatory and
commercialization challenges. However, the horizon is illuminated by emerging technologies, like
personalized medicine, biomarker-driven targeting, and integration with novel therapies, promising
a future where breast cancer treatment is tailored to individual patients and marked by improved
efficacy and reduced side effects.

Conclusion:
In conclusion, this review underscores the transformative potential of polymeric micelle-
based delivery systems in breast cancer therapy.
Possible Terbinafine and/or Itraconazole Induced Vasculitis - A Case Study
Authors: Kumar Sahil , Narayanan Swetha , Kumar Panda Prasan , Chowdhury Arnab 
Published: September 2024

Introduction:
It is essential to exclude other causes, such as autoimmune diseases and
bacterial infections, before attributing cutaneous/systemic vasculitis to drug use.

Case Study:
This report discusses the case of a young man who developed multi-organ failure
and cutaneous vasculitis following the use of antifungal medications (terbinafine and itraconazole)
for dermatophyte infections. Tests for autoimmune diseases and infections were negative.
Given his drug history and a skin biopsy indicating leukocytoclastic vasculitis, it was inferred
that the vasculitis was likely drug-induced. Despite treatment with steroids, intravenous immunoglobulins,
and plasmapheresis, the patient did not survive, possibly due to delayed diagnosis
and treatment.

Conclusion:
In community practice, Drug-induced Vasculitis (DIV) is frequently overlooked.
When patients present with skin rash, fever, and multi-organ dysfunction, DIV should be considered,
particularly in the context of recent drug use. Over-the-counter antifungals, like terbinafine
or itraconazole, can cause DIV and may be fatal if not promptly diagnosed and treated.
Vitamin D Mitigates Depression-related Disorders in an Animal Model of Depression: A Behavioral, Anatomopahtological, and Molecular Investigation, Implicating the Hippocampal BDNF Signaling Pathway
Authors: FILALI Houda , KADIL Youness , RAHMOUNE Imane , Adouani Badr , Badr Latifa , AGOUB Mohamed 
Published: September 2024

Background:
Major depressive disorder represents a complicated mental disorder characterized
by persistent feelings of unhappiness and loss of interest. More evidence suggests a high
potential correlation between vitamin D deficiency and depression. However, the underlying mechanisms
and the therapeutic potential of vitamin D supplementation are still not properly understood.
The purpose of this research is to evaluate the effect of vitamin D supplementation on depressive
behaviors using a rat model of depression and explore the potential mechanisms involved.
Depression is a prevalent mental health disorder that significantly impacts the quality of life of individuals.
Despite the availability of various treatment options, many patients still experience suboptimal
outcomes, highlighting the need for further exploration of novel therapeutic approaches.
In recent years, the importance of vitamin D in mental health, particularly in depression, has
gained considerable attention.

Methods:
In this project, we propose to utilize an animal model of depressed rats to evaluate the
effect of vitamin D supplementation on depressive behaviors. We employed a range of well-established
behavioral tests to assess changes in depressive-like behaviors following vitamin D supplementation.
Additionally, histopathological examinations of the hippocampal region, known to be involved in
mood regulation, were performed to assess structural alterations and cellular changes associated
with depression and vitamin D supplementation.

Results:
The findings demonstrate the therapeutic potential of vitamin D supplementation by improving
neuronal reorganization and proliferation in the hippocampus, suggesting an interest in investigating
other mechanisms of interaction.

Conclusion:
The findings of this research will provide valuable insight into the therapeutic potential
of vitamin D in depression and shed light on the underlying mechanisms involved.
Collaborative Optimization for Active Distribution Network with Soft
Open Point and Energy Storage
Authors: Kewen Li , Lyuzerui Yuan , Shifeng Ou , Shuyin Duan , Qianyi Chen , Tong Liu 
Published: September 2024

Background:
The increasing use of high-penetration distributed clean energy has led to
voltage fluctuations in the distribution network that surpass safety limits and pose challenges to
economic and low-carbon operation. Traditional voltage regulation devices are unable to meet the
real-time high-precision voltage control needs when encountering frequent fluctuations due to
physical limitations. The Soft Open Point (SOP) can provide continuous reactive power to achieve
rapid voltage regulation.

Objective:
We propose a collaborative optimization approach that fully considers the regulatory
capabilities of SOP and energy storage to eliminate voltage violations and reduce network losses.

Methods:
This study introduces an active distribution network reactive power voltage optimization approach that incorporates intelligent SOP and energy storage, combining conventional devices (such as on-load tap changers and capacitor banks) with contemporary devices (such as
SOP, distributed generators, and energy storage) to establish synergy. A coordinated optimization
model was developed, considering various operational constraints to minimize network losses and
regulate the voltage of distribution network nodes. By using linearization and quadratic relaxation,
the original non-convex mixed-integer non-linear optimization model was transformed into a
Mixed-Integer Second-Order Cone Programming (MISOCP) model to meet the requirements of
rapid voltage regulation.

Results:
A case study was conducted on the IEEE 33-node test system, showing that the proposed
approach effectively reduces network losses and eliminates voltage violations.

Conclusion:
The feasibility and effectiveness of the proposed methodology have been validated,
confirming its ability to ensure the safe and economic operation of active distribution networks.