bluuebunny/crossref_metadata_2025_split huggingface dataset:
The design of a legacy indicator tool for measuring climate change related impacts on built heritage
Authors: Daly Cathy 
Published: June 2016

Abstract

Background
We are experiencing a period of climate change the extent and impact of which is uncertain. In the cultural heritage sector the need for monitoring to inform our understanding is widely agreed, yet there is a lack of consensus over what constitutes ‘monitoring for climate change’. This is due, at least in part, to the extended timescales involved. In this paper the design and implementation of one solution is described; a sustainable legacy indicator tool (LegIT) for the long term tracking of surface weathering effects on built heritage.

Results
The assessment of climate change impacts requires 30–100 years of data collection, equal to the period referred to as the ‘climate norm’ by meteorologists. The LegIT is a sacrificial stone object that registers changes in the severity and/or magnitude of weathering patterns on built surfaces, providing a legacy data source for future decision makers. To ensure its sustainability, careful thought was given to the choice of materials, data retrieval and archiving. The tool aims to track surface changes caused by recession, salt crystallisation and microbiological growth.

Conclusion
The development and installation of the LegIT is the first long-term exposure trial to be initiated at heritage sites in Ireland and is intended as a legacy for future researchers.
Complete genome sequence of the haloalkaliphilic, obligately chemolithoautotrophic thiosulfate and sulfide-oxidizing γ-proteobacterium Thioalkalimicrobium cyclicum type strain ALM 1 (DSM 14477T)
Authors: Kappler Ulrike , Davenport Karen , Beatson Scott , Lapidus Alla , Pan Chongle , Han Cliff , Montero-Calasanz Maria del Carmen , Land Miriam , Hauser Loren , Rohde Manfred , Göker Markus , Ivanova Natalia , Woyke Tanja , Klenk Hans-Peter , Kyrpides Nikos C. 
Published: June 2016

AbstractThioalkalimicrobium cyclicum Sorokin et al. 2002 is a member of the family Piscirickettsiaceae in the order Thiotrichales. The γ-proteobacterium belongs to the colourless sulfur-oxidizing bacteria isolated from saline soda lakes with stable alkaline pH, such as Lake Mono (California) and Soap Lake (Washington State). Strain ALM 1T is characterized by its adaptation to life in the oxic/anoxic interface towards the less saline aerobic waters (mixolimnion) of the stable stratified alkaline salt lakes. Strain ALM 1T is the first representative of the genus Thioalkalimicrobium whose genome sequence has been deciphered and the fourth genome sequence of a type strain of the Piscirickettsiaceae to be published. The 1,932,455 bp long chromosome with its 1,684 protein-coding and 50 RNA genes was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program (CSP) 2008.
AQP8 transports NOX2-generated H2O2 across the plasma membrane to promote signaling in B cells
Authors: Bertolotti Milena , Farinelli Giada , Galli Mauro , Aiuti Alessandro , Sitia Roberto 
Published: June 2016

Abstract
H2O2 acts as a second messenger in key signaling circuits, transiently modulating tyrosine phosphatases and kinases. We investigated its origin, membrane transport, and functional role during B cell activation and differentiation. Our data identified NADPH-oxidase 2 as the main source of H2O2 and aquaporin 8 as a transport facilitator across the plasma membrane. On aquaporin 8 silencing, inducible B lymphoma cells responded poorly to TLR and BCR stimulation. Their differentiation was severely impaired, as demonstrated by retarded onset of IgM polymerization, low amounts of IgM secretion, and prolonged BCR expression on the cell surface. A silencing-resistant aquaporin 8 rescued responsiveness, confirming that the import of H2O2 across the membrane is essential for B cell activation. The addition of exogenous catalase to primary B splenocytes severely impaired the tyrosine phosphorylation induced by BCR cross-linking, as did the absence of NOX2 in a murine model of chronic granulomatous disease. Importantly, re-expression of gp91phox through gene therapy restored the specific B cell signaling deficiency in NOX2−/− cells. Thus, efficient induction of B cell activation and differentiation requires intact H2O2 fluxes across the plasma membrane for signal amplification.
Oxidized phosphatidylcholine induces the activation of NLRP3 inflammasome in macrophages
Authors: Yeon Sang Hyeon , Yang Gabsik , Lee Hye Eun , Lee Joo Young 
Published: June 2016

Abstract
The NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome is a multiprotein complex consisting of a receptor, an adaptor protein, and procaspase-1 that induces the secretion of the mature form of IL-1β in response to microbial infection and danger signals. Activation of the NLRP3 inflammasome induced by endogenous danger signal molecules is closely linked to the development and progress of chronic inflammatory diseases. The oxidation of phospholipids occurs upon cellular stress and damage, resulting in the accumulation of oxidized phosphatidylcholines (oxPAPC) such as 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-phosphocholine (POVPC) at inflammatory sites. In this study, we investigated whether oxidized phosphatidylcholine induces the activation of NLRP3 inflammasome in macrophages, leading to the secretion of IL-1β. POVPC induced the degradation of procaspase-1 to caspase-1(p10), the cleavage of pro-IL-1β to IL-1β, and oligomerization of ASC in primary mouse bone marrow-derived macrophages. POVPC-induced production of caspase-1, and IL-1β was abolished in macrophages derived from NLRP3- or caspase-1-deficient mice. In an air pouch model and a peritonitis model in mice, POVPC injection resulted in the production of caspase-1(p10), IL-1β, and IL-18 in wild-type, but not in NLRP3-deficient, mice. POVPC-induced inflammasome activation was mediated by mitochondrial reactive oxygen species resulting from intracellular Ca2+ signaling and mitochondrial destabilization. Our results demonstrate that endogenously produced oxidized phosphatidylcholines such as POVPC induce the activation of NLRP3 inflammasome, leading to the production of IL-1β in macrophages. The results provide an insight to understand how the oxidized lipids endogenously produced upon cellular stress and tissue damage contribute to the inflammatory reaction at pathologic sites.
Expression of human gene coding RORγT receptor depends on the Sp2 transcription factor
Authors: Ratajewski Marcin , Walczak-Drzewiecka Aurelia , Gorzkiewicz Michał , Sałkowska Anna , Dastych Jarosław 
Published: June 2016

Abstract
Th17 cells are involved in the immune response against pathogens, autoimmunity, and tumor progression. The differentiation of human Th17 cells requires the upregulation of RORγT, which in human cells is still not well understood. We identified 2 putative binding motifs for specificity protein transcription factors from the specificity protein/Kruppel-like factor family in the promoter of human RORγT and investigated the involvement of specificity proteins in the transcriptional regulation of this gene. To this end, a human lymphocytic cell line and in vitro-differentiated Th17 cells were used in promoter activity assays, in situ mutagenesis, chromatin immunoprecipitation, and real-time RT-PCR assays. In some experiments, specificity protein expression and activity was inhibited by siRNA and mithramycin A. The results showed that the transcription factor specificity protein 2 recognized binding motifs in the human RORγT promoter, which was critical for maintaining expression. Furthermore, specificity protein 2 was necessary for maximum IL-17 expression in in vitro-differentiated Th17 cells. These observations demonstrate the significant role of specificity protein 2 in the regulation of the Th17 signature transcription factor RORγT and the maintenance of the Th17 phenotype. The findings also suggest that specificity protein 2 plays a role in Th17-dependent physiologic and pathologic immune responses and might serve as a potential novel target for their modulation.
Market Conditions and Performance in the Nursing Home Compare Five-Star Rating
Authors: Kim Ae-Sook 
Published: June 2016

AbstractPrevious studies have documented that market conditions affect nursing home performance; however, the evidence is inconsistent and conflicting. This study introduces three groups of county market conditions and a peer effect variable, and tests their impacts on the Nursing Home Compare (NHC) Five-Star overall rating. Indiana nursing home data and county characteristics are taken mainly from the NHC and Census Bureau websites. The result of the ordered logistic regression analysis indicates that nursing homes in excess demand markets, namely those that are highly concentrated and have fewer nursing homes, tend to perform better than their counterparts in both excess supply and balanced markets. In addition, a peer effect variable, measured as the average overall rating of the competitors, promotes performance improvement. These findings imply that small markets enable consumers to be well informed about a provider's reputation for quality, consequently enhancing performance. Furthermore, not only consumers but also providers seem to seek performance information on the report card to understand their relative position in the market, which thus affects their market strategies and subsequently performance.
Combined cisplatin and aurora inhibitor treatment increase neuroblastoma cell death but surviving cells overproduce BDNF
Authors: Polacchini Alessio , Albani Clara , Baj Gabriele , Colliva Andrea , Carpinelli Patrizia , Tongiorgi Enrico 
Published: June 2016

ABSTRACT
Drug-resistance to chemotherapics in aggressive neuroblastoma (NB) is characterized by enhanced cell survival mediated by TrkB and its ligand, brain-derived neurotrophic factor (BDNF); thus reduction in BDNF levels represent a promising strategy to overcome drug-resistance, but how chemotherapics regulate BDNF is unknown. Here, cisplatin treatment in SK-N-BE neuroblastoma upregulated multiple BDNF transcripts, except exons 5 and 8 variants. Cisplatin increased BDNF mRNA and protein, and enhanced translation of a firefly reporter gene flanked by BDNF 5′UTR exons 1, 2c, 4 or 6 and 3′UTR-long. To block BDNF translation we focused on aurora kinases inhibitors which are proposed as new chemotherapeutics. NB cell survival after 24 h treatment was 43% with cisplatin, and 22% by cisplatin+aurora kinase inhibitor PHA-680632, while the aurora kinases inhibitor alone was less effective; however the combined treatment induced a paradoxical increase of BDNF in surviving cells with strong translational activation of exon6-3′UTR-long transcript, while translation of BDNF transcripts 1, 2C and 4 was suppressed. In conclusion, combined cisplatin and aurora kinase inhibitor treatment increases cell death, but induces BDNF overproduction in surviving cells through an aurora kinase-independent mechanism.
IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA
Authors: Kochan Jakub , Wawro Mateusz , Kasza Aneta 
Published: June 2016

ABSTRACT
MCPIP1 and IER3 are recently described proteins essential for maintenance of immune homeostasis. IER3 is involved in the regulation of apoptosis and differentiation and has been shown lately to protect activated T cells and macrophages from apoptosis. MCPIP1 is an RNase critical for controlling inflammation-related mRNAs. MCPIP1 interacts with and degrades a set of stem-loop-containing mRNAs (including IL-6). Our results demonstrate the involvement of MCPIP1 in the regulation of IER3 mRNA levels. A dual luciferase assay revealed that over-expression of MCPIP1 resulted in a decrease of luciferase activity in the samples co-transfected with constructs containing luciferase CDS attached to IER3 3′UTR. We identified a stem-loop structure similar to that described to be important for destabilization of the IL-6 mRNA by MCPIP1. Examination of IER3 3′UTR sequence, structure and evolutionary conservation revealed that the identified stem-loop is buried within a bigger element. Deletion of this fragment abolished the regulation of IER3 3′UTR-containing transcript by MCPIP1. Finally, using immunofluorescence-combined single-molecule RNA FISH we have shown that the MCPIP1 protein co-localizes with IER3 mRNA. By this method we also proved that the presence of the wild-type NYN/PIN-like domain of MCPIP1 correlated with the decreased level of IER3 mRNA. RNA immunoprecipitation further confirmed the interaction of MCPIP1 with IER3 transcripts in vivo.
An antibody toolkit for the study of membrane traffic inDrosophila melanogaster
Authors: Riedel Falko , Gillingham Alison K. , Rosa-Ferreira Cláudia , Galindo Antonio , Munro Sean 
Published: June 2016

ABSTRACTThe use of Drosophila melanogaster as a model organism has been pivotal to understanding the developmental processes of metazoans. However, the use of flies for studying subcellular organization is hampered by a paucity of reliable reagents to label specific organelles. Here, we describe the generation of mouse monoclonal antibodies against a set of markers of the secretory and endocytic pathways, along with goat polyclonal antibodies against two Golgi proteins. We show that the monoclonal antibodies are highly specific and sufficiently sensitive to detect endogenous proteins in crude extracts by immunoblotting with little background staining. By immunofluorescence the major compartments of the membrane traffic system (including the endoplasmic reticulum, the Golgi, and early and late endosomes) are labeled by at least one antibody. Moreover, the antibodies can be used to label organelles in fly tissues including salivary glands and wing imaginal discs. We anticipate that these antibodies will provide a useful tool kit to facilitate the investigation of how the endomembrane system functions and varies in the diverse tissue types of metazoans.
APPL1 gates long-term potentiation through its plekstrin homology domain
Authors: Fernández-Monreal Mónica , Sánchez-Castillo Carla , Esteban José A. 
Published: June 2016

ABSTRACT
Hippocampal synaptic plasticity involves both membrane trafficking events and intracellular signaling, but how these are coordinated is far from clear. The endosomal transport of glutamate receptors in and out of the postsynaptic membrane responds to multiple signaling cascades triggered by synaptic activity. In this work, we have identified adaptor protein containing a plekstrin homology domain, phosphotyrosine-binding domain and leucine zipper motif 1 (APPL1) as a crucial element linking trafficking and signaling during synaptic plasticity. We show that APPL1 knockdown specifically impairs PI3K-dependent forms of synaptic plasticity, such as long-term potentiation (LTP) and metabotropic-glutamate-receptor-dependent long-term depression (mGluR-LTD). Indeed, we demonstrate that APPL1 is required for the activation of the phosphatidylinositol triphosphate (PIP3) pathway in response to LTP induction. This requirement can be bypassed by membrane localization of PI3K and is related to phosphoinositide binding. Interestingly, inhibitors of PDK1 (also known as PDPK1) and Akt have no effect on LTP expression. Therefore, we conclude that APPL1 gates PI3K activation at the plasma membrane upon LTP induction, which is then relayed by downstream PIP3 effectors that are different from PDK1 and Akt.
The Financial Implications of Supply Chain Changes
Authors: Houston Joel F. , Lin Chen , Zhu Zhongyan 
Published: June 2016

We examine how a firm’s bankruptcy affects the bank financing costs of its key suppliers. We do so by using an extensive, hand-collected data set that captures the supply chain relationships of bankrupt firms over the time period 1990–2009. Looking at a sample of more than 2,000 loan contracts, we compare the average borrowing cost of suppliers in the two years prior to the bankruptcy of a key customer to the average cost in the two years following the announced bankruptcy. We find the average loan spreads increase by roughly 20% following the customer’s announced bankruptcy. These effects are even stronger if the bankrupt firm is operating within a distressed industry or when there is a strong supplier–customer relationship. We also find that the structure of lending agreements significantly changes in the aftermath of a client bankruptcy. More specifically, we find that the number of covenants increases and the lead banker(s) take an increasingly important role in the period following the client’s bankruptcy. Taken together, the results of this study provide new insights into the financial implications of supply chain changes. This paper was accepted by Uday Rajan, finance.
Market Information and Bidder Heterogeneity in Secondary Market Online B2B Auctions
Authors: Pilehvar Ali , Elmaghraby Wedad J. , Gopal Anandasivam 
Published: June 2016

Online auction environments provide several sources of information that can be used by bidders to form their bids. The impact of this information on bidding is likely to be higher when ambiguously specified products are auctioned. The secondary market represents one such environment, where used and returned items from big-box retailers are auctioned off to other business buyers. Using a proprietary data set of secondary market auctions hosted on an online business-to-business platform, we study how market pricing information from other auctions for comparable products affects the bidding behavior of the first bidder. First bidders are shown to be influenced by two sets of pricing information: prices from their own historical bidding behavior and concurrent prices, formed from other open and just-finished auctions relative to the focal auction. We also study how the impact of this market pricing information is moderated by bidder heterogeneity, captured by bidder experience and cross-bidding activity on the platform across comparable concurrent auctions. Finally, we show that the first bid is significantly associated with the auction’s final price, contingent on the type of first bidder. Auctions are increasingly being used for the sale of ill-defined and idiosyncratic products; our work thus provides managerial implications for how auctioneers may design and manage the flow of relevant information to different classes of bidders on their sites in order to enhance their yields. This paper was accepted by Lorin Hitt, information systems.
Parent Perceptions of Museum Participation: A Comparison Between Parents of Children With and Without Autism Spectrum Disorders
Authors: Antonetti Ana , Fletcher Tina 
Published: June 2016

Abstract
This study aims to add to the body of knowledge on the experiences of families of children with autism spectrum disorders (ASD) with the goal of promoting increased participation in community activities. Five factors associated with participation in activities at an art museum were explored, comparing families of children with and without ASD. These factors included both children's frequency of participation and parents' perceptions of the importance of community activities. The differences between groups were statistically significant. Parents of children without ASD reported their children's frequency of participation in museum activities was three times more than parents of children with ASD. Parents of children with ASD reported experiencing three times as many negative emotions associated with museum activities than parents of children without ASD.
Still Caught in the Continuum: A Critical Analysis of Least Restrictive Environment and Its Effect on Placement of Students With Intellectual Disability
Authors: Sauer Janet Story , Jorgensen Cheryl M. 
Published: June 2016

Abstract
The least restrictive environment (LRE) mandate of the Individuals with Disabilities Education Act has long been questioned as to whether it has fulfilled the original intent of the law. This advocacy brief provides an updated analysis of the flaws underlying the principle of LRE, a mandate that exists at the nexus of cultural beliefs about disability, the influence of the medical model on special education, and the misguided link between intensity of services and more restrictive environments. We review the origins of LRE; summarize research on the positive relationship between placement in general education and student outcomes; describe six flaws of LRE's grounding in the continuum model of educational placement that sanctions segregation; present data that illustrate little progress over time towards general education placement for students with intellectual disability, and outline some key court rulings about what constitutes the least restrictive environment. In summary, we suggest that segregation of students with intellectual disability results as much from the flawed underpinnings of the LRE principle itself as on the attitudes and practices of those who use LRE as a justification for segregation.
Supporting Students With Intellectual and Developmental Disabilities in Postsecondary Education: The Motivations and Experiences of Peer Mentors
Authors: Griffin Megan M. , Mello Maria P. , Glover Carrie A. , Carter Erik W. , Hodapp Robert M. 
Published: June 2016

AbstractAlthough peer-mentoring relationships are critical to including youth with intellectual and developmental disabilities in postsecondary educational settings, little is known about the motivations and experiences of peer mentors. To investigate, we conducted a mixed-methods study in which 17 volunteer peer mentors completed the Volunteer Functions Inventory (VFI) and participated in interviews about their motivations and experiences. On the VFI, participants were motivated to volunteer to express personal values and to enhance their own understandings; to a lesser extent, they volunteered to gain career-related experience. Interviews revealed 5 themes: (a) friendships, (b) personal growth, (c) community involvement, (d) experiences with people with disabilities, and (e) future careers related to people with disabilities. Implications for research and practice are discussed.
Dynamika „tworzenia-rynków” w szkolnictwie wyższym
Authors: Komljenovic Janja , Robertson Susan L. 
Published: June 2016

W niniejszym artykule przedstawiamy wyniki badania tego, co wiele osób uznało za dobrze już opracowaną niszę, a mianowicie procesów i rezultatów czegoś, co najczęściej określa się mianem ‘urynkowienia’ sektora szkolnictwa wyższego. Podejmujemy tę kwestię w oparciu o przygotowane przez nas studium przypadku Newton University, w kontekście którego odsłaniamy gwałtowne rozpowszechnienie się wymiany rynkowej między administracyjnymi sekcjami uniwersytetu i jego szerszym otoczeniem. Nasze podejście do procesu „tworzenia-rynku” rozwinięte zostało w dwóch (dialektycznie powiązanych ze sobą) krokach. Po pierwsze, rozpoznajemy zakres wymian rynkowych, wyłonionych w kontekście szerszych ideologicznych i politycznych przemian obszaru ładu instytucjonalnego szkolnictwa wyższego, których celem było uczynienie go bardziej konkurencyjnym wytwórcą wiedzy w skali globalnej, jak również sektorem usługowym. Po drugie, badamy sposoby, w jakie tworzenie rynków wymaga dużego nakładu mikro-pracy, takiej choćby jak zastosowanie szerokiego zakresu określonych ram czy narzędzi socjo-technicznych (Çalışkan, Callon 2009, 2010; Berndt, Boeckler 2012). Ujęte łącznie, wspomniane procesy tworzenia-rynku dokonują ponownego dopasowania i przekształcenia struktury, stosunków społecznych i podmiotowości w obrębie uniwersytetu i poza nim, w ten sposób z kolei konstytuując uniwersytet i sektor szkolnictwa wyższego w nowej formie.