bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chinese L1 children's English L2 verb morphology over time: individual variation in long-term outcomes
Authors: PARADIS JOHANNE , TULPAR YASEMIN , ARPPE ANTTI 
Published: February 2016

AbstractThis study examined accuracy in production and grammaticality judgements of verb morphology by eighteen Chinese-speaking children learning English as a second language (L2) followed longitudinally from four to six years of exposure to English, and who began to learn English at age 4;2. Children's growth in accuracy with verb morphology reached a plateau by six years, where 11/18 children did not display native-speaker levels of accuracy for one or more morphemes. Variation in children's accuracy with verb morphology was predicted by their English vocabulary size and verbal short-term memories primarily, and quality and quantity of English input at home secondarily. This study shows that even very young L2 learners might not all catch up to native speakers in this time frame and that non-age factors play a role in determining individual variation in child L2 learners’ long-term outcomes with English morphology.
The impact of a conditional cash transfer programme on determinants of child health: evidence from Colombia
Authors: Lopez-Arana Sandra , Avendano Mauricio , van Lenthe Frank J , Burdorf Alex 
Published: February 2016

AbstractObjectiveConditional cash transfer (CCT) programmes provide income to low-income families in return for fulfilling specific behavioural conditions. CCT have been shown to improve child health, but there are few systematic studies of their impact on multiple determinants of child health. We examined the impact of a CCT programme in Colombia on: (i) use of preventive health services; (ii) food consumption and dietary diversity; (iii) mother’s knowledge, attitudes and practices about caregiving practices; (iv) maternal employment; and (v) women’s empowerment.DesignSecondary analysis of the quasi-experimental evaluation of theFamilias en Accionprogramme. Children and families were assessed in 2002, 2003 and 2005–06. We applied a difference-in-differences approach using logistic or linear regression, separately examining effects for urban and rural areas.SettingColombia.SubjectsChildren (n1450) and their families in thirty-one treatment municipalities were compared with children (n1851) from sixty-five matched control municipalities.ResultsFamilias en Accionwas associated with a significant increase in the probability of using preventive care services (OR=1·85, 95 % CI 1·03, 3·30) and growth and development check-ups (β=1·36, 95 % CI 0·76, 1·95). It had also a positive impact on dietary diversity and food consumption. No effect was observed on maternal employment, women’s empowerment, and knowledge, attitudes and practices about caregiving practices. Overall,Familias en Accion’s impact was more marked in rural areas.ConclusionCCT in Colombia increase contact with preventive care services and improve dietary diversity, but they are less effective in influencing mother’s employment decisions, empowerment and knowledge of caregiving practices.
Fungal-Induced Deterioration of Mural Paintings:In Situand Mock-Model Microscopy Analyses
Authors: Unković Nikola , Grbić Milica Ljaljević , Stupar Miloš , Savković Željko , Jelikić Aleksa , Stanojević Dragan , Vukojević Jelena 
Published: February 2016

AbstractFungal deterioration of frescoes was studiedin situon a selected Serbian church, and on a laboratory model, utilizing standard and newly implemented microscopy techniques. Scanning electron microscopy (SEM) with energy-dispersive X-ray confirmed the limestone components of the plaster. Pigments used were identified as carbon black, green earth, iron oxide, ocher, and an ocher/cinnabar mixture.In situmicroscopy, applied via a portable microscope ShuttlePix P-400R, proved very useful for detection of invisible micro-impairments and hidden, symptomless, microbial growth. SEM and optical microscopy established that observed deterioration symptoms, predominantly discoloration and pulverization of painted layers, were due to bacterial filaments and fungal hyphal penetration, and formation of a wide range of fungal structures (i.e., melanized hyphae, chlamydospores, microcolonial clusters,Cladosporium-like conidia, andChaetomiumperithecia and ascospores). The all year-round monitoring of spontaneous and induced fungal colonization of a “mock painting” in controlled laboratory conditions confirmed the decisive role of humidity level (70.18±6.91% RH) in efficient colonization of painted surfaces, as well as demonstrated increased bioreceptivity of painted surfaces to fungal colonization when plant-based adhesives (ilinocopie, murdent), compared with organic adhesives of animal origin (bone glue, egg white), are used for pigment sizing.
The Influence of Company Rules, Ethical Climate, and Individual Characteristics on Sales Representative's Honesty
Authors: Grover Steven L. , Enz Cathy A. 
Published: February 2016

ABSTRACTThis study examined the impact of situational and individual characteristics on sales representatives' propensity to lie or to tell the truth. The situational elements were the honesty of the organisation climate and its formal rules about lying to customers. The individual elements were the participants' degree of Machiavellianism and tolerance for ambiguity. The results indicated that more Machiavellian people were more likely to lie and that they were less guided by the rules than people who were low in this trait. In addition, rules and climate work together for people with a high tolerance for ambiguity in a complex manner.
Nitrification and its influence on biogeochemical cycles from the equatorial Pacific to the Arctic Ocean
Authors: Shiozaki Takuhei , Ijichi Minoru , Isobe Kazuo , Hashihama Fuminori , Nakamura Ken-ichi , Ehama Makoto , Hayashizaki Ken-ichi , Takahashi Kazutaka , Hamasaki Koji , Furuya Ken 
Published: February 2016

Abstract
We examined nitrification in the euphotic zone, its impact on the nitrogen cycles, and the controlling factors along a 7500 km transect from the equatorial Pacific Ocean to the Arctic Ocean. Ammonia oxidation occurred in the euphotic zone at most of the stations. The gene and transcript abundances for ammonia oxidation indicated that the shallow clade archaea were the major ammonia oxidizers throughout the study regions. Ammonia oxidation accounted for up to 87.4% (average 55.6%) of the rate of nitrate assimilation in the subtropical oligotrophic region. However, in the shallow Bering and Chukchi sea shelves (bottom ⩽67 m), the percentage was small (0–4.74%) because ammonia oxidation and the abundance of ammonia oxidizers were low, the light environment being one possible explanation for the low activity. With the exception of the shallow bottom stations, depth-integrated ammonia oxidation was positively correlated with depth-integrated primary production. Ammonia oxidation was low in the high-nutrient low-chlorophyll subarctic region and high in the Bering Sea Green Belt, and primary production in both was influenced by micronutrient supply. An ammonium kinetics experiment demonstrated that ammonia oxidation did not increase significantly with the addition of 31–1560 nm ammonium at most stations except in the Bering Sea Green Belt. Thus, the relationship between ammonia oxidation and primary production does not simply indicate that ammonia oxidation increased with ammonium supply through decomposition of organic matter produced by primary production but that ammonia oxidation might also be controlled by micronutrient availability as with primary production.
A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake
Authors: Wang Guozeng , Wang Qiaohuang , Lin Xianju , Bun Ng Tzi , Yan Renxiang , Lin Juan , Ye Xiuyun 
Published: February 2016

AbstractA novel esterase gene (estSL3) was cloned from the Alkalibacterium sp. SL3, which was isolated from the sediment of soda lake Dabusu. The 636-bp full-length gene encodes a polypeptide of 211 amino acid residues that is closely related with putative GDSL family lipases from Alkalibacterium and Enterococcus. The gene was successfully expressed in E. coli and the recombinant protein (rEstSL3) was purified to electrophoretic homogeneity and characterized. rEstSL3 exhibited the highest activity towards pNP-acetate and had no activity towards pNP-esters with acyl chains longer than C8. The enzyme was highly cold-adapted, showing an apparent temperature optimum of 30 °C and remaining approximately 70% of the activity at 0 °C. It was active and stable over the pH range from 7 to 10 and highly salt-tolerant up to 5 M NaCl. Moreover, rEstSL3 was strongly resistant to most tested metal ions, chemical reagents, detergents and organic solvents. Amino acid composition analysis indicated that EstSL3 had fewer proline residues, hydrogen bonds and salt bridges than mesophilic and thermophilic counterparts, but more acidic amino acids and less hydrophobic amino acids when compared with other salt-tolerant esterases. The cold active, salt-tolerant and chemical-resistant properties make it a promising enzyme for basic research and industrial applications.
Using mutagenesis to explore conserved residues in the RNA-binding groove of influenza A virus nucleoprotein for antiviral drug development
Authors: Liu Chia-Lin , Hung Hui-Chen , Lo Shou-Chen , Chiang Ching-Hui , Chen I-Jung , Hsu John T.-A. , Hou Ming-Hon 
Published: February 2016

AbstractNucleoprotein (NP) is the most abundant type of RNA-binding viral protein in influenza A virus–infected cells and is necessary for viral RNA transcription and replication. Recent studies demonstrated that influenza NP is a valid target for antiviral drug development. The surface of the groove, covered with numerous conserved residues between the head and body domains of influenza A NP, plays a crucial role in RNA binding. To explore the mechanism by which NP binds RNA, we performed a series of site-directed mutagenesis in the RNA-binding groove, followed by surface plasmon resonance (SPR), to characterize the interactions between RNA and NP. Furthermore, a role of Y148 in NP stability and NP-RNA binding was evaluated. The aromatic residue of Y148 was found to stack with a nucleotide base. By interrupting the stacking interaction between Y148 and an RNA base, we identified an influenza virus NP inhibitor, (E, E)-1,7-bis(4-hydroxy-3-methoxyphenyl) -1,6-heptadiene-3,5-dione; this inhibitor reduced the NP’s RNA-binding affinity and hindered viral replication. Our findings will be useful for the development of new drugs that disrupt the interaction between RNA and viral NP in the influenza virus.
Genetically engineered flavonol enriched tomato fruit modulates chondrogenesis to increase bone length in growing animals
Authors: Choudhary Dharmendra , Pandey Ashutosh , Adhikary Sulekha , Ahmad Naseer , Bhatia Chitra , Bhambhani Sweta , Trivedi Prabodh Kumar , Trivedi Ritu 
Published: February 2016

AbstractExternally visible body and longitudinal bone growth is a result of proliferation of chondrocytes. In growth disorder, there is delay in the age associated increase in height. The present study evaluates the effect of extract from transgenic tomato fruit expressing AtMYB12 transcription factor on bone health including longitudinal growth. Constitutive expression of AtMYB12 in tomato led to a significantly enhanced biosynthesis of flavonoids in general and the flavonol biosynthesis in particular. Pre-pubertal ovary intact BALB/c mice received daily oral administration of vehicle and ethanolic extract of wild type (WT-TOM) and transgenic AtMYB12-tomato (MYB12-TOM) fruits for six weeks. Animal fed with MYB12-TOM showed no inflammation in hepatic tissues and normal sinusoidal Kupffer cell morphology. MYB12-TOM extract significantly increased tibial and femoral growth and subsequently improved the bone length as compared to vehicle and WT-TOM. Histomorphometry exhibited significantly wider distal femoral and proximal tibial growth plate, increased number and size of hypertrophic chondrocytes in MYB12-TOM which corroborated with micro-CT and expression of BMP-2 and COL-10, marker genes for hypertrophic cells. We conclude that metabolic reprogramming of tomato by AtMYB12 has the potential to improve longitudinal bone growth thus helping in achievement of greater peak bone mass during adolescence.
Disordered RuO2 exhibits two dimensional, low-mobility transport and a metal–insulator transition
Authors: Osofsky M. S. , Krowne C. M. , Charipar K. M. , Bussmann K. , Chervin C. N. , Pala I. R. , Rolison D. R. 
Published: February 2016

AbstractThe discovery of low-dimensional metallic systems such as high-mobility metal oxide field-effect transistors, the cuprate superconductors and conducting oxide interfaces (e.g., LaAlO3/SrTiO3) has stimulated research into the nature of electronic transport in two-dimensional systems given that the seminal theory for transport in disordered metals predicts that the metallic state cannot exist in two dimensions (2D). In this report, we demonstrate the existence of a metal–insulator transition (MIT) in highly disordered RuO2 nanoskins with carrier concentrations that are one-to-six orders of magnitude higher and with mobilities that are one-to-six orders of magnitude lower than those reported previously for 2D oxides. The presence of an MIT and the accompanying atypical electronic characteristics place this form of the oxide in a highly diffusive, strong disorder regime and establishes the existence of a metallic state in 2D that is analogous to the three-dimensional case.
A mirror code for protein-cholesterol interactions in the two leaflets of biological membranes
Authors: Fantini Jacques , Di Scala Coralie , Evans Luke S. , Williamson Philip T. F. , Barrantes Francisco J. 
Published: February 2016

AbstractCholesterol controls the activity of a wide range of membrane receptors through specific interactions and identifying cholesterol recognition motifs is therefore critical for understanding signaling receptor function. The membrane-spanning domains of the paradigm neurotransmitter receptor for acetylcholine (AChR) display a series of cholesterol consensus domains (referred to as “CARC”). Here we use a combination of molecular modeling, lipid monolayer/mutational approaches and NMR spectroscopy to study the binding of cholesterol to a synthetic CARC peptide. The CARC-cholesterol interaction is of high affinity, lipid-specific, concentration-dependent and sensitive to single-point mutations. The CARC motif is generally located in the outer membrane leaflet and its reverse sequence CRAC in the inner one. Their simultaneous presence within the same transmembrane domain obeys a “mirror code” controlling protein-cholesterol interactions in the outer and inner membrane leaflets. Deciphering this code enabled us to elaborate guidelines for the detection of cholesterol-binding motifs in any membrane protein. Several representative examples of neurotransmitter receptors and ABC transporters with the dual CARC/CRAC motifs are presented. The biological significance and potential clinical applications of the mirror code are discussed.
Room-temperature Domain-epitaxy of Copper Iodide Thin Films for Transparent CuI/ZnO Heterojunctions with High Rectification Ratios Larger than 109
Authors: Yang Chang , Kneiß Max , Schein Friedrich-Leonhard , Lorenz Michael , Grundmann Marius 
Published: February 2016

AbstractCuI is a p-type transparent conductive semiconductor with unique optoelectronic properties, including wide band gap (3.1 eV), high hole mobility (>40 cm2 V−1 s−1 in bulk) and large room-temperature exciton binding energy (62 meV). The difficulty in epitaxy of CuI is the main obstacle for its application in advanced solid-state electronic devices. Herein, room-temperature heteroepitaxial growth of CuI on various substrates with well-defined in-plane epitaxial relations is realized by reactive sputtering technique. In such heteroepitaxial growth the formation of rotation domains is observed and hereby systematically investigated in accordance with existing theoretical study of domain-epitaxy. The controllable epitaxy of CuI thin films allows for the combination of p-type CuI with suitable n-type semiconductors with the purpose to fabricate epitaxial thin film heterojunctions. Such heterostructures have superior properties to structures without or with weakly ordered in-plane orientation. The obtained epitaxial thin film heterojunction of p-CuI(111)/n-ZnO(00.1) exhibits a high rectification up to 2 × 109 (±2 V), a 100-fold improvement compared to diodes with disordered interfaces. Also a low saturation current density down to 5 × 10−9 Acm−2 is formed. These results prove the great potential of epitaxial CuI as a promising p-type optoelectronic material.
Internal exposure dynamics drive the Adverse Outcome Pathways of synthetic glucocorticoids in fish
Authors: Margiotta-Casaluci Luigi , Owen Stewart F. , Huerta Belinda , Rodríguez-Mozaz Sara , Kugathas Subramanian , Barceló Damià , Rand-Weaver Mariann , Sumpter John P. 
Published: February 2016

AbstractThe Adverse Outcome Pathway (AOP) framework represents a valuable conceptual tool to systematically integrate existing toxicological knowledge from a mechanistic perspective to facilitate predictions of chemical-induced effects across species. However, its application for decision-making requires the transition from qualitative to quantitative AOP (qAOP). Here we used a fish model and the synthetic glucocorticoid beclomethasone dipropionate (BDP) to investigate the role of chemical-specific properties, pharmacokinetics, and internal exposure dynamics in the development of qAOPs. We generated a qAOP network based on drug plasma concentrations and focused on immunodepression, skin androgenisation, disruption of gluconeogenesis and reproductive performance. We showed that internal exposure dynamics and chemical-specific properties influence the development of qAOPs and their predictive power. Comparing the effects of two different glucocorticoids, we highlight how relatively similar in vitro hazard-based indicators can lead to different in vivo risk. This discrepancy can be predicted by their different uptake potential, pharmacokinetic (PK) and pharmacodynamic (PD) profiles. We recommend that the development phase of qAOPs should include the application of species-specific uptake and physiologically-based PK/PD models. This integration will significantly enhance the predictive power, enabling a more accurate assessment of the risk and the reliable transferability of qAOPs across chemicals.
Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals
Authors: Gao Caixia , Ding Yingying , Zhou Peng , Feng Jiaojiao , Qian Baohua , Lin Ziyu , Wang Lili , Wang Jinhong , Zhao Chunyan , Li Xiangyu , Cao Mingmei , Peng Heng , Rui Bing , Pan Wei 
Published: February 2016

AbstractThe overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA.
Visualization of Surface Acoustic Waves in Thin Liquid Films
Authors: Rambach R. W. , Taiber J. , Scheck C. M. L. , Meyer C. , Reboud J. , Cooper J. M. , Franke T. 
Published: February 2016

AbstractWe demonstrate that the propagation path of a surface acoustic wave (SAW), excited with an interdigitated transducer (IDT), can be visualized using a thin liquid film dispensed onto a lithium niobate (LiNbO3) substrate. The practical advantages of this visualization method are its rapid and simple implementation, with many potential applications including in characterising acoustic pumping within microfluidic channels. It also enables low-cost characterisation of IDT designs thereby allowing the determination of anisotropy and orientation of the piezoelectric substrate without the requirement for sophisticated and expensive equipment. Here, we show that the optical visibility of the sound path critically depends on the physical properties of the liquid film and identify heptane and methanol as most contrast rich solvents for visualization of SAW. We also provide a detailed theoretical description of this effect.
Intrusion of Fukushima-derived radiocaesium into subsurface water due to formation of mode waters in the North Pacific
Authors: Kaeriyama Hideki , Shimizu Yugo , Setou Takashi , Kumamoto Yuichiro , Okazaki Makoto , Ambe Daisuke , Ono Tsuneo 
Published: February 2016

AbstractThe Fukushima Dai-ichi Nuclear Power Plant accident in March 2011 released radiocaesium (137Cs and 134Cs) into the North Pacific Ocean. Meridional transects of the vertical distribution of radiocaesium in seawater were measured along 147 °E and 155 °E in October–November 2012, 19 months after the accident. These measurements revealed subsurface peaks in radiocaesium concentrations at locations corresponding to two mode waters, Subtropical Mode Water and Central Mode Water. Mode water is a layer of almost vertically homogeneous water found over a large geographical area. Here we show that repeated formation of mode water during the two winter seasons after the Fukushima accident and subsequent outcropping into surface water transported radiocaesium downward and southward to subtropical regions of the North Pacific. The total amount of Fukushima-derived 134Cs within Subtropical Mode Water, decay-corrected to April 2011, was estimated to be 4.2 ± 1.1 PBq in October–November 2012. This amount of 134Cs corresponds to 22–28% of the total amount of 134Cs released to the Pacific Ocean.
Hiding true emotions: micro-expressions in eyes retrospectively concealed by mouth movements
Authors: Iwasaki Miho , Noguchi Yasuki 
Published: February 2016

AbstractWhen we encounter someone we dislike, we may momentarily display a reflexive disgust expression, only to follow-up with a forced smile and greeting. Our daily lives are replete with a mixture of true and fake expressions. Nevertheless, are these fake expressions really effective at hiding our true emotions? Here we show that brief emotional changes in the eyes (micro-expressions, thought to reflect true emotions) can be successfully concealed by follow-up mouth movements (e.g. a smile). In the same manner as backward masking, mouth movements of a face inhibited conscious detection of all types of micro-expressions in that face, even when viewers paid full attention to the eye region. This masking works only in a backward direction, however, because no disrupting effect was observed when the mouth change preceded the eye change. These results provide scientific evidence for everyday behaviours like smiling to dissemble and further clarify a major reason for the difficulty we face in discriminating genuine from fake emotional expressions.