bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional diversification of Flowering Locus T homologs in soybean: GmFT1a and GmFT2a/5a have opposite roles in controlling flowering and maturation
Authors: Liu Wei , Jiang Bingjun , Ma Liming , Zhang Shouwei , Zhai Hong , Xu Xin , Hou Wensheng , Xia Zhengjun , Wu Cunxiang , Sun Shi , Wu Tingting , Chen Li , Han Tianfu 
Published: November 2017

Summary

Soybean flowering and maturation are strictly regulated by photoperiod. Photoperiod‐sensitive soybean varieties can undergo flowering reversion when switched from short‐day (SD) to long‐day (LD) conditions, suggesting the presence of a ‘floral‐inhibitor’ under LD conditions.

We combined gene expression profiling with a study of transgenic plants and confirmed that GmFT1a, soybean Flowering Locus T (FT) homolog, is a floral inhibitor.

GmFT1a is expressed specifically in leaves, similar to the flowering‐promoting FT homologs GmFT2a/5a. However, in Zigongdongdou (ZGDD), a model variety for studying flowering reversion, GmFT1a expression was induced by LD but inhibited by SD conditions. This was unexpected, as it is the complete opposite of the expression of flowering promoters GmFT2a/5a. Moreover, the key soybean maturity gene E1 may up‐regulate GmFT1a expression. It is also notable that GmFT1a expression was conspicuously high in late‐flowering varieties. Transgenic overexpression of GmFT1a delayed flowering and maturation in soybean, confirming that GmFT1a functions as a flowering inhibitor.

This discovery highlights the complex impacts of the functional diversification of the FT gene family in soybean, and implies that antagonism between flowering‐inhibiting and flowering‐promoting FT homologs in this highly photoperiod‐sensitive plant may specify vegetative vs reproductive development.
Nutrient‐rich plants emit a less intense blend of volatile isoprenoids
Authors: Fernández‐Martínez Marcos , Llusià Joan , Filella Iolanda , Niinemets Ülo , Arneth Almut , Wright Ian J. , Loreto Francesco , Peñuelas Josep 
Published: November 2017

Summary

The emission of isoprenoids (e.g. isoprene and monoterpenes) by plants plays an important defensive role against biotic and abiotic stresses. Little is known, however, about the functional traits linked to species‐specific variability in the types and rates of isoprenoids emitted and about possible co‐evolution of functional traits with isoprenoid emission type (isoprene emitter, monoterpene emitter or both).

We combined data for isoprene and monoterpene emission rates per unit dry mass with key functional traits (foliar nitrogen (N) and phosphorus (P) concentrations, and leaf mass per area) and climate for 113 plant species, covering the boreal, wet temperate, Mediterranean and tropical biomes.

Foliar N was positively correlated with isoprene emission, and foliar P was negatively correlated with both isoprene and monoterpene emission rate. Nonemitting plants generally had the highest nutrient concentrations, and those storing monoterpenes had the lowest concentrations. Our phylogenetic analyses found that the type of isoprenoid emission followed an adaptive, rather than a random model of evolution.

Evolution of isoprenoids may be linked to nutrient availability. Foliar N and P are good predictors of the type of isoprenoid emission and the rate at which monoterpenes, and to a lesser extent isoprene, are emitted.
A novel PITX2 mutation in non‐syndromic orodental anomalies
Authors: Intarak N , Theerapanon T , Ittiwut C , Suphapeetiporn K , Porntaveetus T , Shotelersuk V 
Published: November 2017

ObjectiveTo identify orodental characteristics and genetic aetiology of a family affected with non‐syndromic orodental anomalies.Subjects and MethodsPhysical and oral features were characterised. DNA was collected from an affected Thai family. Whole‐exome sequencing was employed to identify the pathogenic variants associated with inherited orodental anomalies. The presence of the identified mutation was confirmed by Sanger sequencing.ResultsWe observed unique orodental manifestations including oligodontia, retained primary teeth, taurodont molars, peg‐shaped maxillary central incisors, high attached frenum with nodule and midline diastema in the proband and her mother. Mutation analyses revealed a novel heterozygous frameshift deletion, c.573_574delCA, p.L193QfsX5, in exon 5 of PITX2A in affected family members. The amino acid alterations, localised in the transcriptional activation domain 2 in the C‐terminus of PITX2, were evolutionarily conserved. Mutations in PITX2 have been associated with autosomal‐dominant Axenfeld–Rieger syndrome and non‐syndromic eye abnormalities, but never been found to cause isolated oral anomalies.ConclusionsThis study for the first time demonstrates that the PITX2 mutation could lead to non‐syndromic orodental anomalies in humans. We propose that the specific location in the C‐terminal domain of PITX2 is exclusively necessary for tooth development.
Association of dental and maxillary sinus pathologies with ear, nose, and throat symptoms
Authors: Horwitz Berkun R , Polak D , Shapira L , Eliashar R 
Published: November 2017

ObjectivesTo investigate the correlation between cone‐beam computerized tomography (CBCT) findings in the maxillary sinus, ear–nose–throat (ENT) symptoms and dental pathologies in asymptomatic patients.Materials and MethodsA total 81 patients were referred for CBCT and filled a standard ENT visual analog scale (VAS) questionnaire. CBCT images were analyzed for sinus ostium obstruction, Schneiderian membrane thickening, sinus floor turbidity, and the presence of polyps. Dental pathologies were evaluated with the aid of CBCT images, periapical X‐rays, and clinical examination. A possible correlation between the CBCT findings and the ENT/dental parameters was examined by applying Student's t test and the chi‐squared test.ResultsDespite being asymptomatic, most of the 81 patients reported ENT symptoms in the questionnaire, thereby indicating that these symptoms were mainly subclinical. A significant correlation was found between the presence of polyps in the sinus and a decrease in smell/taste. Obstruction of the sinus meatus was associated with coughing; turbidity was associated with ear congestion. Thickening of the Schneiderian membrane showed an association with both coughing and ear congestion. The mean number of missing posterior teeth correlated with postnasal drip and nasal congestion. Periapical pathology was associated with nasal discharge/runny nose.ConclusionThe results emphasize the need to evaluate ENT symptoms when radiographic findings are identified in CBCT.
Objective measurement of spectacle wear with a temperature sensor data logger
Authors: Lentsch Matthew J. , Marsack Jason D. , Anderson Heather A. 
Published: November 2017

AbstractPurposeThis study seeks to establish the utility of the SmartButton Data Logger (www.acrsystems.com) to monitor spectacle wear for research and clinical applications.MethodsFifty adults wore a thermosensor on their spectacles for 2 weeks for each of two mount types while keeping wear‐time logs. Temperatures during reported spectacle wear (ON) were compared to temperatures during non‐wear (OFF) with repeated measures analysis of variance (ANOVA). In addition, two strategies to approximate spectacle wear from temperature data were evaluated: (1) Filtering data based on temperature ranges to identify spectacle wear (either group mean ON temperature, or an individual's mean ON temperature), and (2) Separate examiners inspecting temperature against time plots to identify spectacle wear. The success of these methods to approximate wear time was evaluated by per cent error with respect to subject reported wear time.ResultsGroup mean ON (31.8 [0.6]°Celsius [°C]) and OFF (24.7 [1.5]°C) temperatures differed significantly (F1,47 = 471.2, p < 0.001), but there was no difference in temperature between mounts (F1,47 = 1.9, p = 0.18). Median per cent error and first and third quartiles (Q1, Q3) of each technique used to approximate wear time were: group mean filtering = 8% (Q1 3%, Q3 18%), individual mean filtering = 7% (Q1 4%, Q3 19%), Examiner 1 = 6% (Q1 2%, Q3 14%), Examiner 2 = 7% (Q1 3%, Q3 12%).ConclusionsThe SmartButton can monitor spectacle compliance in patients with all approximation methods evaluated providing less than 10% median per cent error in wear time.
Genomic analysis of maize lines introduced in the early stages of a breeding programme
Authors: Cantelmo Narjara Fonseca , Von Pinho Renzo Garcia , Balestre Marcio 
Published: November 2017

AbstractThe diversity analysis and screening of major genes using genomewide association studies (GWAS) for quantitative traits have been exploited in several grass crops, but in maize it has been neglected. This study was conducted to evaluate the genetic diversity of a collection of lines and the potential of genomic analysis as an initial screening tool. For this purpose, a collection of 470 maize inbred lines were genotyped with 23,153 Dart‐seq markers. The genomewide analysis of ear weight was used aiming to perform an initial screening in our germplasm. The diversity analysis allowed the lines to be separated into distinct groups, and most of the lines were in agreement with previous pedigree information. The GWAS revealed ten regions potentially associated with the ear weight trait, one of which was related to drought stress. The results of a multiple marker analysis confirmed four of these regions, presenting a predictive accuracy of 0.6. Based on the molecular data and the results of GWAS, it is possible to infer the potential use of this genetic background for breeding.
Identification of potential gene‐associated major traits using GBS‐GWAS for Korean apple germplasm collections
Authors: Lee So Jin , Ban Seung Hyun , Kim Gi Hoon , Kwon Soon Il , Kim Jeong Hee , Choi Cheol 
Published: November 2017

AbstractGenomewide association study (GWAS), which queries the association between loci and a particular trait by examining single nucleotide polymorphisms (SNPs) of the entire genome, is used in many fields of study. The development of next‐generation sequencing techniques has facilitated GWASs by decreasing the sequencing costs and time. In particular, genotyping by sequencing (GBS) is useful for sequencing many samples simultaneously and at a moderate price. Herein, we describe a potential GWAS using GBS, focused on the apple germplasm, with the goal of developing an effective apple breeding strategy through the identification of useful markers. From 308 Korean apple germplasm, SNPs were selected after GBS, and major traits were investigated. Proprietary individuals were confirmed and grouped by association through genetic diversity and population structure analyses of the selected SNPs. Genes highly associated with the target traits were identified, respectively. As the first GWAS report on the apple germplasm, these results will be useful as base data for GWASs on other apple populations and traits.
Ultrasound findings in idiopathic facial aseptic granuloma: Case series and literature review
Authors: Knöpfel Nicole , Gómez‐Zubiaur Alba , Noguera‐Morel Lucero , Torrelo Antonio , Hernandez‐Martin Angela 
Published: November 2017

AbstractIdiopathic facial aseptic granuloma is an inflammatory nodule commonly located on the cheeks and eyelids in young children. Despite its prolonged course, it tends toward spontaneous resolution, so invasive diagnostic procedures should be avoided. Cutaneous ultrasound is a noninvasive modality that has been found to improve the diagnostic accuracy of nodular skin lesions. We report five children with idiopathic facial aseptic granuloma in whom high‐resolution ultrasound examination provided distinctive findings.
RASA1 mutation in a family with capillary malformation–arteriovenous malformation syndrome: A discussion of the differential diagnosis
Authors: Edwards Lisa R. , Blechman Adam B. , Zlotoff Barrett J. 
Published: November 2017

AbstractWe describe a family who presented with several scattered, vascular, cutaneous lesions and was found to have a novel mutation in RASA1, diagnostic of capillary malformation–arteriovenous malformation syndrome. Our patient was initially given a presumptive clinical diagnosis of hereditary hemorrhagic telangiectasia. Capillary malformation–arteriovenous malformation syndrome shares several features with hereditary hemorrhagic telangiectasia and hereditary benign telangiectasia, but it can be distinguished clinically according to its morphologic appearance and distribution of cutaneous vascular lesions, the presence of internal fast‐flow lesions, and genetic analysis.
Evaluating the feasibility of the steady‐state visual evoked potential (SSVEP) to study temporal attention
Authors: Mora‐Cortes Anderson , Ridderinkhof K. Richard , Cohen Michael X 
Published: November 2017

AbstractImprovements in perceptual performance can be obtained when events in the environment are temporally predictable—and temporal predictability improves attention and sensory processing. The amplitude of the steady‐state visual evoked potential (SSVEP) has been shown to correlate with attention paid to a flickering stimulus even if the flickering stimulus is irrelevant for the task. However, to our knowledge, the validity of the SSVEP to study temporal attention has not been established. Therefore, we designed an SSVEP temporal attention task to evaluate whether the SSVEP and its temporal dynamics can be used to study temporal attention. We used a forced‐choice perceptual detection task while presenting task‐irrelevant visual flicker at alpha (10 Hz) and two surrounding frequencies (6 or 15 Hz). Temporal predictability was manipulated by having the interstimulus intervals (ISI) be constant or variable. Behavioral results replicated previous studies confirming the benefits of temporal expectations on performance for trials with constant ISI. EEG analyses revealed robust SSVEP amplitudes for all flicker frequencies, although a main effect of temporal expectations on SSVEP amplitude was not significant. Additional analyses revealed temporal predictability‐related modulations of SSVEP amplitude at 10 Hz and its second harmonic (20 Hz). The effect of temporal predictability was also observed for the 6 Hz flicker, but not for 15 Hz for any ISI condition. These results provide some evidence for the feasibility of the SSVEP technique to study temporal attention for stimuli with flicker frequencies around the alpha band.
Oral healthcare in Fraser syndrome
Authors: Hassona Y. , Kharoub H. , Scully C. 
Published: November 2017

ABSTRACTFraser syndrome is a rare autosomal recessive disorder characterized by renal agenesis, cryptophthalmos, syndactyly, and laryngeal stenosis. Oral manifestations are sparsely described in the literature, and patients with this syndrome might require special dental management considerations because of the associated cardiac, renal, and cognitive defects. In this report, we describe the oral/dental findings in a 22‐year‐old girl with Fraser syndrome, and discuss the dental management.
Sinusitis caused by Exserohilum rostratum after cord blood transplantation for myelodysplastic syndrome: A case report and literature review
Authors: Kohashi Sumiko , Toyama Takaaki , Hashimoto Norisato , Sakurai Masatoshi , Kato Jun , Kikuchi Taku , Koda Yuya , Sugita Kayoko , Hasegawa Naoki , Yarita Kyoko , Kamei Katsuhiko , Okamoto Shinichiro , Mori Takehiko 
Published: November 2017

AbstractInvasive fungal disease is a serious infectious complication of allogeneic hematopoietic stem cell transplantation (HSCT). Exserohilum rostratum is a species causing phaeohyphomycosis, which rarely causes invasive disease in humans. We treated a case of sinusitis caused by E. rostratum after cord blood transplantation (CBT). A 60‐year‐old man with myelodysplastic syndrome, who had a medical history of an operation to correct deviation of the nasal septum, developed sinusitis caused by E. rostratum under prolonged profound neutropenia after a second CBT because of the graft rejection of the first transplantation. Liposomal amphotericin B improved the sinusitis. A literature review revealed nine reported cases of sinusitis caused by E. rostratum, including our case. Although five cases had severe neutropenia at onset (HSCT recipients, n = 2; aplastic anemia, n = 3), the remaining four had no preexisting immunosuppressive conditions. However, three of the four patients had preexisting nasal diseases with or without a history of surgery, as in our case. Excluding our case, the outcome was fatal in five neutropenic patients, whereas the four patients without neutropenia recovered. Although sinusitis caused by E. rostratum is rare, E. rostratum should be recognized as a possible pathogen causing sinusitis in highly immunosuppressed patients such as HSCT recipients. Preexisting nasal disease and/or nasal surgery could be risks for this infection.
Association between baseline serum hepcidin levels and infection in kidney transplant recipients: Potential role for iron overload
Authors: Fernández‐Ruiz Mario , Parra Patricia , Ruiz‐Merlo Tamara , López‐Medrano Francisco , San Juan Rafael , Polanco Natalia , González Esther , Andrés Amado , Aguado José María 
Published: November 2017

AbstractBackgroundThe liver‐synthesized peptide hepcidin is a key regulator of iron metabolism and correlates with total iron stores. We analyzed the association between pre‐transplant hepcidin‐25 levels and infection after kidney transplantation (KT).MethodsSerum hepcidin‐25 levels were measured at baseline by high‐sensitivity ELISA in 91 patients undergoing KT at our institution between December 2011 and March 2013. The impact of this biomarker on the incidence of post‐transplant infection (excluding lower urinary tract infection) during the first year was assessed by Cox regression.ResultsMean hepcidin‐25 level was 82.3 ± 67.4 ng/mL and strongly correlated with serum ferritin (Spearman's rho = 0.703; P < .001). There were no significant differences in hepcidin‐25 levels between patients with or without overall infection (96.4 ± 67.5 vs 72.6 ± 66.7 ng/mL; P = .101). Such difference was evident for opportunistic (128.9 ± 75.0 vs 73.0 ± 62.3 ng/mL; P = .003) and, to a lesser extent, surgical‐site infection (107.5 ± 73.3 vs 76.5 ± 65.2 ng/mL; P = .087). Patients with hepcidin‐25 levels ≥72.5 ng/mL had higher 12‐month cumulative incidence of overall infection (51.2% vs 29.2%; P = .032). After multivariate adjustment, hepcidin‐25 ≥72.5 ng/mL acted as an independent risk factor for overall (adjusted hazard ratio [aHR] 3.86; 95% confidence interval [CI] 1.49‐9.96; P = .005) and opportunistic infection (aHR 4.32; 95% CI 1.18‐15.75; P = .027).ConclusionElevated baseline serum hepcidin‐25 levels were associated with increased risk of infection after KT, suggesting a role for iron overload in the individual susceptibility to post‐transplant infection.
Can Escherichia coli fly? The role of flies as transmitters of E. coli to food in an urban slum in Bangladesh
Authors: Lindeberg Yrja Lisa , Egedal Karen , Hossain Zenat Zebin , Phelps Matthew , Tulsiani Suhella , Farhana Israt , Begum Anowara , Jensen Peter Kjær Mackie 
Published: November 2017

AbstractObjectiveTo investigate the transmission of faecal bacteria by flies to food under natural settings.MethodsOver a period of 2 months, paired (exposed and non‐exposed) containers with cooked rice were placed on the ground in kitchen areas in an urban slum area in Dhaka, Bangladesh, and the numbers of flies landing on the exposed rice were counted. Following exposure, the surface of the rice was microbiologically and molecularly analysed for the presence of Escherichia coli and genes of diarrhoeagenic E. coli and Shigella strains.ResultsRice was at greater risk (P < 0·001) of being contaminated with E. coli if flies landed on the rice than if no flies landed on the rice (odds ratio 5·4 (P < 0·001, 95% CI: 2·5–11·7). Mean contamination in exposed rice samples (n = 60) was 3·1 × 103 CFU/g (95% CI: 2·2 × 103–4·0 × 103). Furthermore, for approximately half of the observed fly landings, the average CFU per fly landing was >0·6 × 103 CFU. Genes of diarrhoeagenic E. coli and Shigella species were detected in 39 of 60 (65%) of exposed rice samples. Two fly species were identified: the common housefly (Musca domestica) and the oriental latrine fly (Chrysomya megacephala).ConclusionFlies may transmit large quantities of E. coli to food under field settings. The findings highlight the importance of implementing control measures to minimise exposure of food to flies to ensure food safety. Fly control measures should be considered for the prevention of diarrhoeal diseases caused by E. coli.
Occurrence of hemolytic reactions on the same day as immune globulin product administrations during 2008 to 2014
Authors: Sridhar Gayathri , Ekezue Bola F. , Izurieta Hector S. , Forshee Richard A. , Selvam Nandini , Mintz Paul D. , Anderson Steven A. , Menis Mikhail D. 
Published: November 2017

BACKGROUNDHemolytic reactions (HRs) are rare serious adverse events after immune globulin (IG) use. Our large claims‐based study evaluated occurrence of same‐day hemolysis after administration of different IG products and potential risk factors, during the 2008 to 2014 study period.STUDY DESIGN AND METHODSWe conducted a retrospective cohort study using a large commercial administrative database. The study included individuals exposed to IG products as identified by procedure codes. HRs were ascertained using ICD‐9‐CM diagnosis codes. Unadjusted same‐day hemolysis rates (per 1000 persons) were estimated overall, by age, sex, and IG products. Multivariable regression analyses were used to evaluate potential risk factors.RESULTSOf 20,440 persons exposed, 211 (10.3 per 1000) had same‐day HRs. The median numbers of doses for IG users with versus without same‐day hemolysis were one and six, respectively. The unadjusted product‐specific HR rates ranged from 1.92 for subcutaneous product Hizentra to 17.99 for intravenous Octagam. The multivariable regression analyses showed significantly increased same‐day HR risk in males and in IG users with histories of hemolysis, pneumonia, and hereditary hemolytic anemias. Compared to Gammagard Liquid, significantly elevated overall hemolysis risk was identified with Octagam (odds ratio, 2.36; 95% confidence interval, 1.04‐5.35), using Firth's method to account for small sample size bias.CONCLUSIONThe study showed variation in the same‐day IG‐related hemolysis by age, sex, and IG products administered. The results suggest importance of underlying health conditions, especially prior hemolysis, and first IG product dose. Differences in HR occurrence may also be explained by product manufacturing processes, indications, routes, and rates of administration, which warrant further investigation.