bluuebunny/crossref_metadata_2025_split huggingface dataset:
Region specific drivers of cerebrospinal fluid mobility as measured by high-resolution non-invasive MRI in humans
Authors: Hirschler Lydiane , Runderkamp Bobby A. , Decker Andreas , Harten Thijs W. van , Scheyhing Paul , Ehses Philipp , Petitclerc Léonie , Nordsiek Julia , Pracht Eberhard , Coolen Bram F. , Zwaag Wietske van der , Stöcker Tony , Vollmuth Philipp , Paech Daniel , Effland Alexander , Walderveen Marianne A.A. van , Radbruch Alexander , Buchem Mark A. van , Petzold Gabor C. , Veluw Susanne J. van , Caan Matthan W.A. , Deike* Katerina , Osch* Matthias J.P. van 
Published: October 2024

Abstract

Highlights

CSF-mobility can be measured in humans down to the level of
perivascular spaces surrounding penetrating vessels using
CSF-STREAM
In perivascular spaces surrounding penetrating vessels, the
cardiac and respiratory cycles have similar effects on
CSF-mobility
Entraining vasomotion at 0.1 Hz can drive CSF-mobility in
humans
CSF-mobility is altered in patients with cerebral amyloid
angiopathy

Abstract
Many neurological diseases are characterized by the accumulation of toxic proteins in the brain. This accumulation has been associated with improper clearance from the parenchyma. Recent discoveries highlighted perivascular spaces, which are cerebrospinal fluid (CSF)-filled spaces, as the channels of brain clearance. The forces driving CSF-mobility within perivascular spaces are still debated. Here, we present a new, non-invasive, CSF-specific magnetic resonance imaging technique (CSF-STREAM), that enables detailed in vivo measurement of CSF-mobility in humans, for the first time down to the level of perivascular spaces located around penetrating vessels, i.e. close to protein production sites. We find region-specific drivers for CSF-mobility and demonstrate that CSF-mobility can be increased by entraining vasomotion. Furthermore, we found region-specific CSF-mobility alterations in patients with cerebral amyloid angiopathy, a brain disorder associated with clearance impairment. The availability of this new technique opens up avenues to investigate the impact of CSF-mediated clearance in neurodegeneration and sleep.
Volatile Oil Composition, Phytochemical Screening and Insecticidal Activities of Indigofera tinctoria Against African Pink Worm Borer
Authors: Olaoluwa OLAOLUWA O. , ALABI Olajumoke Y , Isiaka MOHAMMED , AYODELE A. Maria , Guido FLAMINI , TAIWO Olayombo M. 
Published: October 2024

Abstract

The following study presents the constituents of the volatile oils found in the leaves and seeds of Indigofera tinctoria, as well as the secondary metabolites of its leaves crude fractions. The insecticidal activities of these fractions against Sesamia calamistis were also evaluated. The volatile oils from the leaf and seed of Indigofera tinctoria were extracted using the hydro-distillation method, utilizing an all-glass Clevenger apparatus. The extracted oils were then characterized using the Gas Chromatography-Mass Spectrometry (GC-MS) technique. The n-hexane, ethyl acetate and ethanol fractions of I. tinctoria were also investigated for secondary metabolites using standard qualitative phytochemical analysis methods. The insecticidal activities of these fractions against Sesamia calamistis were evaluated using a contact toxicity test procedure. The results of the study showed that Phytol (34.1%) and 1-octen-3-ol (63.8%) were the major constituents of the leaf and seed oil of I. tinctoria, respectively. Saponins were present in the hexane and ethanol fractions, while terpenoids and tannins were present in the ethyl acetate and ethanol fractions. The ethyl acetate fraction showed the highest percentage of S. calamistis larvae mortality of 70.0% at a 0.4% concentration compared to the n-hexane and ethanol fractions. Therefore, the ethyl acetate fraction of I. tinctoria leaf at a 0.4% concentration with the highest percentage of larvae mortality could be used to manage Sesamia calamistis on maize.
Visual Performance and Patient Satisfaction with Multifocal, Monofocal and Monofocal-Plus Intraocular Lenses
Authors: Ben-Eli Hadas , Wunch Inbar , Maroko Riki , Winnett Tamar , Berrih-Elkouby Deborah , Marcovitch Arie 
Published: October 2024

Abstract

Objective
To evaluate the visual performance and patient satisfaction of three intraocular lenses (IOLs): the newly-designed monofocal-plus Tecnis Eyhance, multifocal Tecnis Synergy, and monofocal Sensar (Johnson & Johnson Surgical Vision©, Inc. Santa Ana, CA, USA).
Methods
This prospective, comparative, interventional study enrolled patients undergoing cataract surgery with implantation of Eyhance, Sensar, or Synergy IOLs. Postoperative uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) were assessed at distance (6m), intermediate (70 cm), and near (40 cm) in LogMAR units. Contrast sensitivity (CS) was measured using the Pelli-Robson chart at distance and near vision. Subjective visual comfort was evaluated through a validated questionnaire.
Results
The study included 52 participants (52% females; 84 eyes): 40 eyes with Eyhance, 26 with Synergy, and 18 with Sensar. Ages ranged from 48 to 82 years (mean age 70.2 ± 7.59 years), with 59% female participants. There was no significant difference in distance UCVA among the IOL types (P > 0.8). Synergy showed superior intermediate and near UCVA compared to Eyhance and Sensar (P < 0.01). Eyhance and Sensar demonstrated better distance CS than Synergy (P ≤ 0.05) and better near CS (P < 0.05). Questionnaire responses showed no significant difference in overall visual comfort, but higher satisfaction with intermediate vision was reported for Synergy.
Conclusions
The Synergy lens is recommended for patients prioritizing intermediate and near vision. Eyhance did not show an objective advantage over Sensar for intermediate vision and contrast sensitivity (CS) but had a subjective advantage. Despite reduced CS, Synergy provided excellent distance UCVA, similar to Eyhance and Sensar.
Comparative volatiles profiling in Marjoram products essential oil as extracted using classical and headspace techniques and in relation to antioxidant and antibacterial effects
Authors: Baky Mostafa , Abouelela Mostafa , Shawky Enas , Farag Mohamed , Elgendy Omayma 
Published: October 2024

Abstract

Recently, the growth of consumer demand for natural herbal products with both safety and efficacy has led to great advances in analytical tools to assess and assure their quality. Marjoram (Origanum majorana L.), also known as "sweet marjoram" or “sweet oregano” is a Mediterranean herbaceous perennial herb cultivated in Egypt. The main goal of this study was to assess volatiles’ variation in marjoram samples collected from two different commercial products using two different extraction techniques viz. HS-SPME and petroleum ether coupled with GC-MS analysis. A total of 20 major aroma compounds were identified in samples extracted with HS-SPME with abundance of monoterpene hydrocarbons and oxygenated compounds. The major volatiles included β-phellandrene (20.1 and 14.2%), γ-terpinene (13.4 and 11.7%), 2-bornene (12.3 and 11.5%), p-cymene (9.8% and 4.6%) terpenen-4-ol (16.4% and 7.5%), sabinene hydrate (16.02% and 8.8%) and terpineol (4.2 and 3.2%) in MR and MI, respectively. Compared with HS-SPME, 51 aroma compounds were identified in marjoram samples extracted with pet. ether, more enriched in aliphatic hydrocarbons (42.8 and 73.8%) in MR and MI, respectively. While higher identification score was observed in case of solvent extraction, SPME appeared to be more selective in recovery of oxygenated terpenes to more account for marjoram aroma. The total phenolic and flavonoid contents in marjoram samples were at (111.9, 109.1 µg GA/mg) and (18.3, 19.5 µg rutin eq/mg) in MR and MI, respectively. Stronger antioxidant effects were observed in MR and MI samples with IC50 at 45.5 and 56.8 µg/mL respectively compared to IC50 6.57 µg/mL for trolox as assayed using DPPH assay. Moderate anti-bacterial effect was observed in MR and MI samples and expressed as zone of inhibition mostly against Bacillus subtilis (16.03 & 15.9 mm), B. cereus (12.9 & 13.7mm), Enterococcus faecalis (14.03 & 13.97 mm), and Enterobacter cloacae (11.60 & 11.56 mm) respectively.
Dissociated response and treatment outcome with immune checkpoint blockade in advanced cancer
Authors: Guan Yaping , Cui Yu , Gong Yanhong , Liang Xiuju , Han Xinyue , Chen Yingcui , Xie Hong , Zhang Yuekai , Wang baocheng , Ye xin , Wang Jun 
Published: October 2024

Abstract

Immune-related dissociated response (DR) has been recently recognized and have become a subject of ongoing interest. The purpose of the present study was to evaluate the frequency, treatment outcome, and predictors of DR in cancer patients with immune checkpoint inhibitors. We retrospectively collected clinicopathological data from a cohort of patients with cancer who received PD-1/PD-L1 inhibitor-based monotherapy or combination therapy at a single institution (developing cohort). An independent cohort of advanced non-small cell lung cancer (NSCLC) patients treated with immunotherapy at two institutions was used as the validating cohort. Progression‐free survival (PFS) and overall survival (OS) were used as outcome measures. The pantumor cohort included 177 patients. DR were observed in 12 (6.8%) patients. The median PFS and OS were significantly longer in patients with atypical response versus nonresponse but shorter versus true response. Patients with DR had a longer median PFS and OS than those with true PD. Local treatment seemed to have a positive influence on DR patient outcomes, with a median OS of 32.3 months versus 21.9 months for no local treatment. No clinical characteristics remained significant predictors for DR. In the NSCLC cohort, DR was observed in 10 (12.5%) patients. Inferior PFS and OS were validated in patients with real PD when compared with patients with DR. Patients who experience DR exhibit a relatively favorable prognosis. Some patients with DR may benefit from thecontinuation of ICI administration and local treatment to the growing lesions and achieve a longer survival.
Effects of Perinatal Variables on Echocardiographic Assessments of Left Ventricular Dimensions in Infants Born Large for Gestational Age: A Prospective Cohort Analysis
Authors: Amarah Ahmed , Elmakaty Ibrahim , Nadroo Iram , Chhabra Manoj , Hoang Danthanh , Suk Debbie , Nadroo Ali M , Ron Nitin , Dygulska Beata , Gudavalli Madhu B , Narula Pramod , Gad Ashraf 
Published: October 2024

Abstract

Background
To assess the relationship between perinatal factors, and echocardiographic left ventricular (LV) dimensions after delivery in infants who are large for gestational age (LGA).
Methods
This is a prospective cohort study that was conducted between 2014 and 2018, and involved healthy LGA newborns born > 35 weeks’ gestation, delivered at New York-Presbyterian Brooklyn Methodist Hospital, and a control group of appropriate for gestational age (AGA) infants. Data analysis was performed using multivariate linear regression in STATA.
Results
A total of 563 neonates were enrolled in this study. They were composed of 414 AGA infants as the control group and 149 LGA infants as the intervention group. The male sex was predominant in both groups. A larger proportion of neonates were admitted to the neonatal intensive care unit (NICU) in LGA infants (74.6%) as compared to the AGA infants (33.5%) (p < 0.001). In the study's regression analysis, birth weight (BW) emerged as a key factor, positively correlating with increased LV mass, interventricular septum thickness, and LV posterior wall thickness across both LGA and AGA. Additionally, BW showed a positive correlation with left ventricular internal dimensions in diastole and systole. Higher maternal BMI was associated with an increase in fractional shortening in LGA infants, while maternal insulin use during pregnancy was positively associated with interventricular septum thickness. Notably, male infants exhibited significantly higher LV internal dimensions in both diastole and systole, while GA negatively impacted the left ventricular mass-to-volume ratio.
Conclusions
The study's findings underscore the significant influence of perinatal factors on neonatal cardiac morphology, in both LGA and AGA infants. BW, GA, gender, maternal BMI, and maternal insulin use during pregnancy were key determinants affecting various aspects of LV structure, including mass, wall thickness, and internal dimensions. These insights highlight the importance of considering these perinatal factors in the assessment and monitoring of neonatal cardiac health, offering valuable guidance for tailored clinical approaches in pediatric cardiology.
What is the state of play in adulthood?
Authors: Colston Rosemary , Millear Prudence , Katsikitis Mary , Keech Jacob 
Published: October 2024

Abstract

This qualitative study explored the experiences of self-identified adult players to gain insights into the state of play in adulthood. Interviews were conducted with 26 participants representing different styles of play behaviour. Thematic analysis using Nvivo 12 Plus software revealed that adults play differently from children and have a broader definition of play behaviour. Play was found to be rewarding and provided relief from adult responsibilities. The most skilled players integrated play into their work-life seamlessly. The analysis also identified a typology of 8 player types, influenced by parental attitudes and behaviours during childhood. Participants emphasised the importance of play for promoting wellbeing, although there was a degree of play illiteracy evidenced. Further research is needed to investigate if the benefits of play can be extended to the general population through facilitated engagement with remedial play and the development of an adult play discourse.
Characterization of a periodontal-inflammatory microRNA profile during long-term multibracket orthodontic treatment in adolescents - a split-mouth design study
Authors: Rolfes Florian , Heck Johannes , Riedel Isabelle , Bär Christian , Schmitz Boris 
Published: October 2024

Abstract

This study aimed to identify functional microRNAs (miRNAs) and their respective targets as central regulatory factors of tooth movement during orthodontic treatment.
Gingival crevicular fluid (GCF) of 24 adolescent patients (< 18 years) treated with a full-mouth multibracket appliance (MBA; Thermal Copper Nickel Titanium archwire) was analyzed for miRNAs-21, -29b, -34a, -126, -132, -146a, and -221 in a split-mouth study design. GCF samples were taken from either jaw (second maxillary, mandibular premolar) using non-invasive sampling before, 7 days, 5 weeks, and 3 months after application of orthodontic force (8 samples per patient). Validated miRNA targets and regulated pathways were identified using the miRTarBase database (release 9.0) and Reactome (version 87).
All analyzed miRNAs were consistently detected in the GCF (Ct value < 35) and a moderate to high correlation was found between samples taken from the mandible and maxilla before treatment (r = 0.42 to 0.71, all p ≤ 0.041). All miRNAs showed changes in their expression levels with orthodontic tooth movement compared to baseline (significant time effect, all p < 0.001). The general profile indicated an increase in miRNA expression in both jaws with time with the exeption of miR-21, which showed reduced levels one week after MBA application (p = 0.046). For miR-34, a significant interaction effect was observed (time × jaw, p = 0.0396) in that lower levels were found after five weeks and three months of treatment in the mandible compared to the maxilla. The medium to late treatment phase was characterized by an increase in miR-146 and miR-221. Gene signaling pathway analysis suggested regulation of cellular response to stress including hypoxia, matrix reorganization and vascular remodeling.
Since the identified miRNA profile was linked to targets involved in the remodeling process of the alveolar supporting apparatus and alveolar bone, GCF-derived miRNAs may represent diagnostic biomarkers to monitor cellular processes during orthodontic tooth movement and potentially optimize individual treatment outcomes.
Long-term outcomes of a bioactive matrix enriched with an autologous platelet concentrate for the treatment of complex anal fistulae
Authors: Maya Irene , Spada Eliana , Martí-Gallostra Marc , Tudela Arturo Cirera , Pellino Gianluca , Espín-Basany Eloy 
Published: October 2024

Abstract

Background
Treating complex anal fistulae (CAF) remains a clinical challenge. Regenerative Fistula Treatment (RFT) with a bioactive matrix enriched with autologous platelet concentrate (Obsidian RFT™) has shown potential in healing of CAF while preserving continence, but its long-term efficacy is still debated. This study aimed to report the outcomes of RFT in patients with CAF.
Methods
A retrospective analysis of a prospective database of patients with CAF treated with RFT between February 2021 and November 2023 at a single centre was conducted. Patients were included if they completed at least a 6-month follow-up. The primary outcome was fistula healing at last available follow-up. Secondary outcomes included unscheduled reoperation and anorectal function.
Results
Thirty-one patients were treated with Obsidian RFT, 17 of whom completed the 6-month-follow-up. Nine of the patients were women. The median age was 47 (24–63) years, and eight had inflammatory bowel disease. High trans-sphincteric fistulae were observed in 52.9% of patients. At a median follow-up of 21.8 (8–36) months, a 53% success rate was achieved. Approximately half of patients required at least one additional procedure due to CAF persistence or recurrence. There were no reports of morbidity and mortality, and no worsening of continence was observed.
Conclusions
This pilot study demonstrated that nearly half of the patients treated with RFT for CAF achieved long-term healing, with no adverse effects on continence and an excellent safety profile. Larger studies are needed to draw definitive conclusions.
Association Between Platelet to High-Density Lipoprotein Cholesterol Ratio and Risk of Diabetes and Prediabetes: Recent Findings from NHANES 2005– 2018
Authors: Chen Pengfei , Zhu Meilin , Shi Dazhuo , Du Jianpeng 
Published: October 2024

Abstract

Purpose: To explore the relationship between the platelet-to-high-density lipoprotein cholesterol ratio (PHR) and the risk of diabetes and prediabetes.
Methods:This study analyzes data from the 2005-2018 National Health and Nutrition Examination Survey (NHANES). The prevalence of diabetes and prediabetes, as well as levels of HDL-C and platelet counts, were derived from cross-sectional surveys. The PHR was calculated by dividing platelet count by HDL-C concentration, and diabetes or prediabetes were classified according to established clinical criteria. We used multivariate logistic regression analyses to estimate odds ratios (ORs) and 95% CIs. The logistic regression models were classified into categorical and continuous models. The potential non-linear relationship was assessed using restricted cubic splines (RCSs) and two-piecewise linear regression to identify any inflection points. Additionally, subgroup and interaction analyses were conducted to determine variations across different population groups.
Result:A total of 20,229 eligible participants were included in the study, with a mean age of 47.84 years, and 51.80% of them were female. Among these participants, 3,884 (14.29%) were diagnosed with diabetes, and 8,863 (44.36%) were prediabetes. The result showed a positive association between PHR and the risk of diabetes and prediabetes. After adjusting for model 3, the OR for diabetes and prediabetes was associated with a per unit increase in PHR of 1.14 (95% CI: 1.00–1.29, P<0.05). The OR for participants in the highest PHR quartile was 2.46 (95% CI: 1.34–4.51, P<0.01) compared to those in the lowest quartile. Two-piecewise regression analysis identified a breakpoint at PHR = 4.55, with a positive association observed when PHR was below this value (OR = 1.32, 95% CI: 1.01–1.73, P<0.05). Subgroup and interaction analyses demonstrated that the positive association remained consistent across various demographic groups.
Conclusions: Our study indicates that a higher PHR may be associated with an increased risk of developing diabetes and prediabetes. Therefore, PHR could potentially be used as a marker for assessing the likelihood of these conditions.
Non-transecting anastomotic augmented uretroplasty with dorsal onlay buccal graft for the treatment of bulbous urethral strictures: Results and complications
Authors: Baudry Alexandre , Schirmann Aurélie , Guillot-Tantay Cyrille , Lebret Thierry , Neuville Paul , Boillot Bernard , Madec François-Xavier 
Published: October 2024

Abstract

Introduction : Urethroplasty is the gold standard for the management of urethral strictures. For obliterative bulbar strictures longer than 2 cm, excision primary anastomosis (EPA) cannot be optimally performed, and staged urethroplasty is recommended. To minimize multiple interventions, Augmented Anastomotic Urethroplasty (AAU) was developed. Incorporating the vessel-sparing approach without the transection of the spongiosum would improve the results of urethroplasty and limit complications. Our study aimed to assess the outcomes of non-transecting Anastomotic Augmented Urethroplasty with a buccal graft (ntAAU). Method : From 2019 to 2023, 42 patients underwent ntAAU. Anatomical success was defined as the ability to pass a cystoscope (16,5 Fr) and functional success was determined by a maximum urinary flow rate (Qmax) greater than 15 ml/s. Clinical outcomes were evaluated using patient reported outcome concerning urinary function (USP dysuria, VPPS, six items LUTS, questions 7, 9, 10 from USS-PROM) and erectile function (IIEF-5, EHS). Results : Anatomical success rate was 90.5% and functional success was 83.3% with a median (IQR) follow-up of 18 (12.5–28.5) months. The complication (urinary infection, suprapubic catheter obliteration, abscess, bleeding) rate was 14.2%. Questionnaires were avaiblable for 31 patients. Median (IQR) USP dysuria score was 0 (0–1), VPPS score was 7 (3–12), and six-item LUTS score was 4 (2–9). The median (IQR) IIEF-5 score was 22 (15.5–25) and EHS score was 3.5 (2–4). Patients with treatment failure were managed with redo-urethroplasties. Conclusion : This technique shows good outcomes and appears to be a valuable option for managing long strictures with an obliterative part.