bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple capacitive method to evaluate ethanol fuel samples
Authors: Vello Tatiana P. , de Oliveira Rafael F. , Silva Gustavo O. , de Camargo Davi H. S. , Bufon Carlos C. B. 
Published: February 2017

AbstractEthanol is a biofuel used worldwide. However, the presence of excessive water either during the distillation process or by fraudulent adulteration is a major concern in the use of ethanol fuel. High water levels may cause engine malfunction, in addition to being considered illegal. Here, we describe the development of a simple, fast and accurate platform based on nanostructured sensors to evaluate ethanol samples. The device fabrication is facile, based on standard microfabrication and thin-film deposition methods. The sensor operation relies on capacitance measurements employing a parallel plate capacitor containing a conformational aluminum oxide (Al2O3) thin layer (15 nm). The sensor operates over the full range water concentration, i.e., from approximately 0% to 100% vol. of water in ethanol, with water traces being detectable down to 0.5% vol. These characteristics make the proposed device unique with respect to other platforms. Finally, the good agreement between the sensor response and analyses performed by gas chromatography of ethanol biofuel endorses the accuracy of the proposed method. Due to the full operation range, the reported sensor has the technological potential for use as a point-of-care analytical tool at gas stations or in the chemical, pharmaceutical, and beverage industries, to mention a few.
Spatial complexity enhances predictability in food webs
Authors: Mougi Akihiko 
Published: February 2017

AbstractThe prediction of an ecosystem’s response to an environmental disturbance or the artificial control of ecosystems is a challenging task in ecology. Ecological theory predicts that disturbances frequently result in unexpected responses between interacting species due to the many indirect interactions within a complex community. However, such indeterminacy appears to be unusual in nature. Here using a meta-community food web, I show that spatiality is key to resolving this disparity. A moderate level of spatial coupling strength between habitats due to species migration increases the possibility of expected responses to press perturbation or predictability. Moreover, predictability increases with increasing spatial complexity, as measured by the number of local food webs and their connectivity. A meta-community network can attenuate the propagation of disturbances through indirect pathways due to species emigration to other habitats, thereby preserving the expected effect on the interacting species. These results suggest that the isolation of communities due to habitat destruction decreases the predictability of communities, thereby complicating the control of ecosystems.
Multivariate analysis of factors associated with kyphotic deformity after laminoplasty in cervical spondylotic myelopathy patients without preoperative kyphotic alignment
Authors: Cao JunMing , Zhang JingTao , Yang DaLong , Yang Liu , Shen Yong 
Published: February 2017

AbstractThe risk factors of post-laminoplasty kyphosis in patients with cervical spondylotic myelopathy (CSM) without preoperative kyphotic alignment are not well known. This study aimed to compare clinical and radiological data between patients with or without post-laminoplasty kyphosis and to investigate the factors associated with post-laminoplasty kyphosis in CSM patients without preoperative kyphotic alignment. Patients (n = 194) who received unilateral expansive open-door cervical laminoplasty with miniplate fixation and completed a 1-year follow-up were enrolled. Patients were grouped according to whether they suffered from postoperative kyphosis (P) or not (NP). Postoperative kyphosis was observed in 21 (10.8%) patients. The recovery rates of the Japanese Orthopaedic Association scores at the 1-year follow-up in the P group were inferior to those in the NP group (31.9% vs. 65.2%, P < 0.001). Logistic regression with post-laminoplasty kyphosis as the dependent variable showed independent risks associated with an increased C2–7 sagittal vertical axis (SVA, odds ratio [OR] = 1.085, 95% confidence interval [CI] = 1.025–1.203, P = 0.015), destroyed facet joints (OR = 1.132, 95% CI = 1.068–1.208, P < 0.001), and cephalad vertebral level undergoing laminoplasty (CVLL, OR = 2.860, 95% CI = 1.164–6.847, P = 0.021). These findings suggest that CVLL, C2–7 SVA, and destroyed facet joints are associated with kyphosis after laminoplasty in CSM patients without preoperative kyphotic alignment.
Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences
Authors: Crescenzo-Chaigne Bernadette , Barbezange Cyril V. S. , Léandri Stéphane , Roquin Camille , Berthault Camille , van der Werf Sylvie 
Published: February 2017

AbstractFor each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences and specific, non-conserved sequences. The latter and adjacent parts of the coding sequence are involved in genome packaging, but the precise role of the non-conserved NC sequences is still unclear. The aim of this study is to better understand the role of the non-conserved non-coding sequences in the incorporation of the viral segments into virions. The NA-segment NC sequences were systematically replaced by those of the seven other segments. Recombinant viruses harbouring two segments with identical NC sequences were successfully rescued. Virus growth kinetics and serial passages were performed, and incorporation of the viral segments was tested by real-time RT-PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were restored. Sequencing revealed that the replacing 5′NC sequence length drove the type of mutations obtained. With sequences longer than the original, point mutations in the coding region with or without substitutions in the 3′NC region were detected. With shorter sequences, insertions were observed in the 5′NC region. Restoration of viral fitness was linked to restoration of the NA segment incorporation.
Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations
Authors: Gao Xiang , Yu Ting , Xu Guangya , Guo Gang , Liu Xiaoxiao , Hu Xin , Wang Xiang , Liu Yanhui , Mao Qing , You Chao , Zhou Liangxue 
Published: February 2017

AbstractCombination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro. In addition, honokiol and doxorubicin loaded in MPEG-PCL nanoparticles could efficiently suppress glioma cell proliferation and induce cell apoptosis in vitro. Furthermore, Dox-HK-MPEG-PCL micelles inhibited glioma growth more significantly than Dox-MPEG-PCL and HK-MPEG-PCL in both nude mice and zebrafish tumor models. Immunohistochemical analysis indicated that DOX-HK-MPEG-PCL micelles improved Dox’s anti-tumor effect by enhancing tumor cell apoptosis, suppressing tumor cell proliferation, and inhibiting angiogenesis. Our data suggest that Dox-HK-MPEG-PCL micelles have the potential to be applied clinically in glioma therapy.
Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells
Authors: Dumitrache Alexandru , Klingeman Dawn M. , Natzke Jace , Rodriguez Jr Miguel , Giannone Richard J. , Hettich Robert L. , Davison Brian H. , Brown Steven D. 
Published: February 2017

AbstractClostridium (Ruminiclostridium) thermocellum is a model organism for its ability to deconstruct plant biomass and convert the cellulose into ethanol. The bacterium forms biofilms adherent to lignocellulosic feedstocks in a continuous cell-monolayer in order to efficiently break down and uptake cellulose hydrolysates. We developed a novel bioreactor design to generate separate sessile and planktonic cell populations for omics studies. Sessile cells had significantly greater expression of genes involved in catabolism of carbohydrates by glycolysis and pyruvate fermentation, ATP generation by proton gradient, the anabolism of proteins and lipids and cellular functions critical for cell division consistent with substrate replete conditions. Planktonic cells had notably higher gene expression for flagellar motility and chemotaxis, cellulosomal cellulases and anchoring scaffoldins, and a range of stress induced homeostasis mechanisms such as oxidative stress protection by antioxidants and flavoprotein co-factors, methionine repair, Fe-S cluster assembly and repair in redox proteins, cell growth control through tRNA thiolation, recovery of damaged DNA by nucleotide excision repair and removal of terminal proteins by proteases. This study demonstrates that microbial attachment to cellulose substrate produces widespread gene expression changes for critical functions of this organism and provides physiological insights for two cells populations relevant for engineering of industrially-ready phenotypes.
Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae
Authors: Uthe Henriette , Vanselow Jens T. , Schlosser Andreas 
Published: February 2017

AbstractHere we present the most comprehensive analysis of the yeast Mediator complex interactome to date. Particularly gentle cell lysis and co-immunopurification conditions allowed us to preserve even transient protein-protein interactions and to comprehensively probe the molecular environment of the Mediator complex in the cell. Metabolic 15N-labeling thereby enabled stringent discrimination between bona fide interaction partners and nonspecifically captured proteins. Our data indicates a functional role for Mediator beyond transcription initiation. We identified a large number of Mediator-interacting proteins and protein complexes, such as RNA polymerase II, general transcription factors, a large number of transcriptional activators, the SAGA complex, chromatin remodeling complexes, histone chaperones, highly acetylated histones, as well as proteins playing a role in co-transcriptional processes, such as splicing, mRNA decapping and mRNA decay. Moreover, our data provides clear evidence, that the Mediator complex interacts not only with RNA polymerase II, but also with RNA polymerases I and III, and indicates a functional role of the Mediator complex in rRNA processing and ribosome biogenesis.
Water flow buffers shifts in bacterial community structure in heat-stressed Acropora muricata
Authors: Lee Sonny T. M. , Davy Simon K. , Tang Sen-Lin , Kench Paul S. 
Published: February 2017

AbstractDeterioration of coral health and associated change in the coral holobiont’s bacterial community are often a result of different environmental stressors acting synergistically. There is evidence that water flow is important for a coral’s resistance to elevated seawater temperature, but there is no information on how water flow affects the coral-associated bacterial community under these conditions. In a laboratory cross-design experiment, Acropora muricata nubbins were subjected to interactive effects of seawater temperature (27 °C to 31 °C) and water flow (0.20 m s−1 and 0.03 m s−1). In an in situ experiment, water flow manipulation was conducted with three colonies of A. muricata during the winter and summer, by partially enclosing each colony in a clear plastic mesh box. 16S rRNA amplicon pyrosequencing showed an increase in the relative abundance of Flavobacteriales and Rhodobacterales in the laboratory experiment, and Vibrio spp. in the in situ experiment when corals were exposed to elevated temperature and slow water flow. In contrast, corals that were exposed to faster water flow under laboratory and in situ conditions had a stable bacterial community. These findings indicate that water flow plays an important role in the maintenance of specific coral-bacteria associations during times of elevated thermal stress.
Origin of asymmetric broadening of Raman peak profiles in Si nanocrystals
Authors: Gao Yukun , Yin Penggang 
Published: February 2017

AbstractThe asymmetric peak broadening towards the low-frequency side of the Raman-active mode of Si nanocrystals with the decreasing size has been extensively reported in the literatures. In this study, an atomic coordination model is developed to study the origin of the ubiquitous asymmetric peak on the optical phonon fundamental in the Raman spectra of Si nanocrystals. Our calculation results accurately replicate the line shape of the experimentally measured optical Raman curves. More importantly, it is revealed that the observed asymmetric broadening is mainly caused by the surface bond contraction and the quantum confinement.
The Utilization of Social Media in the Hearing Aid Community
Authors: Choudhury Moumita , Dinger Zoë , Fichera Elena 
Published: February 2017

Purpose
This study investigated the utilization of social media by the hearing aid (HA) community. The purpose of this survey was to analyze the participation of HA community in the social media websites.

Method
A systematic survey of online HA-related social media sources was conducted. Such sources were identified using appropriate search terms. Social media participation was quantified on the basis of posts and “likes.”

Results
Five hundred fifty-seven social media sources were identified, including 174 Twitter accounts, 172 YouTube videos, 91 Facebook pages, 20 Facebook groups, 71 blogs, and 29 forums. Twitter and YouTube platforms showed the highest level of activity among social media users. The HA-related community used social media sources for advice and support, information sharing, and service-related information.

Conclusions
HA users, other individuals, and organizations interested in HAs leave their digital footprint on a wide variety of social media sources. The community connects, offers support, and shares information on a variety of HA-related issues. The HA community is as active in social media utilization as other groups, such as the cochlear implant community, even though the patterns of their social media use are different because of their unique needs.
Laryngeal Aerodynamics in Healthy Older Adults and Adults With Parkinson's Disease
Authors: Matheron Deborah , Stathopoulos Elaine T. , Huber Jessica E. , Sussman Joan E. 
Published: February 2017

Purpose
The present study compared laryngeal aerodynamic function of healthy older adults (HOA) to adults with Parkinson's disease (PD) while speaking at a comfortable and increased vocal intensity.

Method
Laryngeal aerodynamic measures (subglottal pressure, peak-to-peak flow, minimum flow, and open quotient [OQ]) were compared between HOAs and individuals with PD who had a diagnosis of hypophonia. Increased vocal intensity was elicited via monaurally presented multitalker background noise.

Results
At a comfortable speaking intensity, HOAs and individuals with PD produced comparable vocal intensity, rates of vocal fold closure, and minimum flow. HOAs used smaller OQs, higher subglottal pressure, and lower peak-to-peak flow than individuals with PD. Both groups increased speaking intensity when speaking in noise to the same degree. However, HOAs produced increased intensity with greater driving pressure, faster vocal fold closure rates, and smaller OQs than individuals with PD.

Conclusions
Monaural background noise elicited equivalent vocal intensity increases in HOAs and individuals with PD. Although both groups used laryngeal mechanisms as expected to increase sound pressure level, they used these mechanisms to different degrees. The HOAs appeared to have better control of the laryngeal mechanism to make changes to their vocal intensity.
Unterschiede in der Selbst- und Fremdbeurteilung gesundheitsbezogener Lebensqualität bei Patienten mit leichter kognitiver Beeinträchtigung und Demenz vom Alzheimer-Typ
Authors: Heßmann Philipp , Dreier Maren , Brandes Iris , Dodel Richard , Baum Erika , Müller Matthias , Balzer-Geldsetzer Monika 
Published: February 2017

Zusammenfassung
Ziel Patienten mit einer Alzheimer-Demenz (AD) und leichten kognitiven Beeinträchtigungen (LKB) beurteilen ihre gesundheitsbezogene Lebensqualität (LQ) häufig besser als ihre Angehörigen. Einflussfaktoren dieses Beurteilungsunterschieds werden evaluiert.
Methodik Die LQ von 241 zu Hause lebenden Patienten wurde anhand des krankheitsspezifischen Quality of Life-Alzheimer’s Disease-Fragebogens (QoL-AD) erhoben.
Ergebnisse Das Verwandtschaftsverhältnis, depressive Symptome und die Alltagsfunktionalität stellen relevante Einflussfaktoren bei Beurteilungsunterschieden der LQ dar.
Schlussfolgerung Soziodemografische und klinische Variablen können Bewertungsunterschiede der LQ von Patienten mit AD und LKB teilweise erklären.
Considerations for the Full Global Withdrawal of Oral Polio Vaccine After Eradication of Polio
Authors: Hampton Lee M. , du Châtellier Gaël Maufras , Fournier-Caruana Jacqueline , Ottosen Ann , Rubin Jennifer , Menning Lisa , Farrell Margaret , Shendale Stephanie , Patel Manish 
Published: February 2017

Abstract
Eliminating the risk of polio from vaccine-derived polioviruses is essential for creating a polio-free world, and eliminating that risk will require stopping use of all oral polio vaccines (OPVs) once all types of wild polioviruses have been eradicated. In many ways, the experience with the global switch from trivalent OPV (tOPV) to bivalent OPV (bOPV) can inform the eventual full global withdrawal of OPV. Significant preparation will be needed for a thorough, synchronized, and full withdrawal of OPV, and such preparation would be aided by setting a reasonably firm date for OPV withdrawal as far in advance as possible, ideally at least 24 months. A shorter lead time would provide valuable flexibility for decisions about when to stop use of OPV in the context of uncertainty about whether or not all types of wild polioviruses had been eradicated, but it might increase the cost of OPV withdrawal.
New ALMA constraints on the star-forming interstellar medium at low metallicity: a 50 pc view of the blue compact dwarf galaxy SBS 0335−052
Authors: Cormier D. , Bendo G. J. , Hony S. , Lebouteiller V. , Madden S. C. , Galliano F. , Glover S. C. O. , Klessen R. S. , Abel N. P. , Bigiel F. , Clark P. C. 
Published: February 2017

Abstract
Properties of the cold interstellar medium of low-metallicity galaxies are not well known due to the faintness and extremely small scale on which emission is expected. We present deep ALMA band 6 (230 GHz) observations of the nearby, low-metallicity (12 + log (O/H) = 7.25) blue compact dwarf galaxy SBS 0335−052 at an unprecedented resolution of 0.2 arcsec (52 pc). The 12CO J = 2→1 line is not detected and we report a 3σ upper limit of LCO(2–1) = 3.6 × 104 K km s−1 pc2. Assuming that molecular gas is converted into stars with a given depletion time, ranging from 0.02 to 2 Gyr, we find lower limits on the CO-to-H2 conversion factor αCO in the range 102–104 M⊙ pc−2 (K km s−1)−1. The continuum emission is detected and resolved over the two main super star clusters. Re-analysis of the IR–radio spectral energy distribution suggests that the mm-fluxes are not only free–free emission but are most likely also associated with a cold dust component coincident with the position of the brightest cluster. With standard dust properties, we estimate its mass to be as large as 105 M⊙. Both line and continuum results suggest the presence of a large cold gas reservoir unseen in CO even with ALMA.
Emerging Techniques in Degenerative Thoracolumbar Surgery
Authors: Choma Theodore J. , Mroz Thomas E. , Goldstein Christina L. , Arnold Paul , Shamji Mohammed F. 
Published: February 2017

Abstract
There continue to be incremental advances in thoracolumbar spine surgery techniques in attempts to achieve more predictable outcomes, minimize risk of complications, speed recovery, and minimize the costs of these interventions. This paper reviews recent literature with regard to emerging techniques of interest in the surgical treatment of lumbar spinal stenosis, fusion fixation and graft material, degenerative lumbar spondylolisthesis, and thoracolumbar deformity and sacroiliac joint degeneration. There continue to be advances in minimal access options in these areas, although robust outcome data are heterogeneous in its support. The evidence in support of sacroiliac fusion appears to be growing more robust in the properly selected patient.
Resection of Navigated Transcranial Magnetic Stimulation-Positive Prerolandic Motor Areas Causes Permanent Impairment of Motor Function
Authors: Moser Tobias , Bulubas Lucia , Sabih Jamil , Conway Neal , Wildschutz Noémie , Sollmann Nico , Meyer Bernhard , Ringel Florian , Krieg Sandro M. 
Published: February 2017

Abstract

BACKGROUND: Navigated transcranial magnetic stimulation (nTMS) helps to determine the distribution of motor eloquent areas prior to brain surgery. Yet, the eloquence of primary motor areas frontal to the precentral gyrus identified via nTMS is unclear.

OBJECTIVE: To investigate the resection of nTMS-positive prerolandic motor areas and its correlation with postsurgical impairment of motor function.

METHODS: Forty-three patients with rolandic or prerolandic gliomas (WHO grade I-IV) underwent nTMS prior to surgery. Only patients without ischemia within the motor system in postoperative MRI diffusion sequences were enrolled. Based on the 3-dimensional fusion of preoperative nTMS motor mapping data with postsurgical MRI scans, we identified nTMS points that were resected in the infiltration zone of the tumor. We then classified the resected points according to the localization and latency of their motor evoked potentials. Surgery-related paresis was graded as transient (≤6 weeks) or permanent (>6 weeks).

RESULTS: Out of 43, 31 patients (72%) showed nTMS-positive motor points in the prerolandic gyri. In general, 13 out of 43 patients (30%) underwent resection of nTMS points. Ten out of these patients showed postoperative paresis. There were 2 (15%) patients with a transient and 8 (62%) with a permanent surgery-related paresis. In 3 cases (23%), motor function remained unimpaired.

CONCLUSION: After resection of nTMS-positive motor points, 62% of patients suffered from a new permanent paresis. Thus, even though they are located in the superior or middle frontal gyrus, these cortical areas must undergo intraoperative mapping.