bluuebunny/crossref_metadata_2025_split huggingface dataset:
Status and conservation of the Endangered snow leopard Panthera uncia in Qomolangma National Nature Reserve, Tibet
Authors: Chen Pengju , Gao Yufang , Wang Jun , Pu Qiong , Lhaba Cering , Hu Huijian , Xu Jian , Shi Kun 
Published: July 2016

AbstractLittle is known about the status of the snow leopard Panthera uncia in Qomolangma National Nature Reserve, located on the northern aspect of Mount Everest in Tibet. To address this, during May–September 2014 we conducted line transects, camera trapping, household interviews, and socioeconomic statistics analysis. We surveyed 14 transects and located 287 putative snow leopard signs, with a mean density of 1.9 sign sites km–1, 3.8 signs km–1, and 1.4 scrapes km–1. We set 41 camera traps and recorded a minimum of seven individual snow leopards. Our results were comparable to snow leopard abundance estimates for neighbouring protected areas in Nepal. Semi-structured interviews with 46 (59%) households found that local people were generally supportive of snow leopard conservation, for a variety of economic, legislative, and religious reasons. The socio-economic situation in the Reserve underwent dramatic changes between 2000 and 2014. The human population increased by 28.9%, the livestock population decreased by 9.9%, the number of tourists in 2014 was 6.8 times greater than in 2005, and the local gross domestic product underwent an annual increase of 15%. We discuss the current threats to snow leopards, and recommend that more rigorous, comprehensive, and interdisciplinary research be undertaken to provide an evidential basis for the formulation of effective conservation policies and programmes.
Moral Performance and Cultural Governance in China: The Compassionate Politics of Disasters
Authors: Xu Bin 
Published: July 2016

AbstractThis article examines the Chinese state's moral performance during several major disasters, including the 2008 Sichuan earthquake, the 1998 Yangtze River floods, and the 1976 Tangshan earthquake. Drawing on the theatrical theory of symbolic politics, I argue that the Sichuan earthquake marked a turn in the state's moral performance. While the Chinese state continued to project an image of a secure, heroic state, it endeavoured to construct a sympathetic image through leaders' displays of compassion and sorrow, a mourning ritual for ordinary victims, and narratives of response and rescue. This shift towards a more compassionate performance can be explained by the state's deployment of cultural resources to respond to societal challenges since the new millennium and its effort to repair its image amid the crises of 2008. The compassionate performance was temporarily effective because it found common ground with the traditional political culture of disaster, which still shapes the public's expectations of the state's moral conduct, and the new public culture that values equality and dignity of human life. Nevertheless, the political dilemmas of the compassionate performance became evident. Its efficacy largely relied on the presentation of suffering at the scene, which, however, led to public demands for the state to address the causes of the suffering. When the state failed to construct an “accountable state” image, this “dilemma of scene” had repercussions for its legitimacy. The efficacy of paternalism was also limited because it was less appealing to the growing urban middle class. By addressing moral performance, this paper contributes to the literature on politics of disaster and advances the important research agenda on cultural governance.
The Labour Law System, Capitalist Hegemony and Class Politics in China
Authors: Hui Elaine Sio-ieng 
Published: July 2016

AbstractThis article investigates how the Chinese labour law system has helped to reproduce capitalist hegemony, i.e. the ethico-political, moral and cultural leadership of the ruling class. Based on intensive fieldwork in the Pearl River Delta and 115 interviews with migrant workers, this article shows that the labour law system has exercised a double hegemonic effect with regards to capital–labour relations and state–labour relations. Through normalizing, countervailing, concealing and transmuting mechanisms, the labour law system has been able to buffer both the market economy and the party-state from workers’ radical and fundamental criticism. However, the double hegemony mediated through the labour law system has influenced the Chinese migrant workers in an uneven manner: some of them have granted active consent to the ruling class leadership; some have only rendered passive consent; and some have refused to give any consent at all.
Digital stories: Bringing multimodal texts to the Spanish writing classroom
Authors: Oskoz Ana , Elola Idoia 
Published: July 2016

AbstractDespite the availability and growing use of digital story software for authoring and instructional purposes, little is known about learners’ perceptions on its integration in the foreign language writing class. Following both a social semiotics approach and activity theory, this study focuses on six advanced Spanish learners’ perceptions about the production of a digital story in which they integrated a variety of modes (written, oral, images, sounds) and manipulated the semiotic resources within each mode (size, color, lines in the image mode), to convey meaning. Analyzing participants’ reflections, questionnaires, and online journals, results highlight learners’ (a) interpretation of the tools and artifacts and their effect on their understanding of a final product, (b) connections between short-term goal-oriented actions and the longer-term object-oriented activity of developing a multimodal text, and (c) linguistic reorientations when creating a digital story.
Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling
Authors: Nichterwitz Susanne , Chen Geng , Aguila Benitez Julio , Yilmaz Marlene , Storvall Helena , Cao Ming , Sandberg Rickard , Deng Qiaolin , Hedlund Eva 
Published: July 2016

AbstractLaser capture microscopy (LCM) coupled with global transcriptome profiling could enable precise analyses of cell populations without the need for tissue dissociation, but has so far required relatively large numbers of cells. Here we report a robust and highly efficient strategy for LCM coupled with full-length mRNA-sequencing (LCM-seq) developed for single-cell transcriptomics. Fixed cells are subjected to direct lysis without RNA extraction, which both simplifies the experimental procedures as well as lowers technical noise. We apply LCM-seq on neurons isolated from mouse tissues, human post-mortem tissues, and illustrate its utility down to single captured cells. Importantly, we demonstrate that LCM-seq can provide biological insight on highly similar neuronal populations, including motor neurons isolated from different levels of the mouse spinal cord, as well as human midbrain dopamine neurons of the substantia nigra compacta and the ventral tegmental area.
The Maternal Effect Genes UTX and JMJD3 Play Contrasting Roles in Mus musculus Preimplantation Embryo Development
Authors: Yang Lei , Song Li-Shuang , Liu Xue-Fei , Xia Qing , Bai Li-Ge , Gao Li , Gao Guang-Qi , Wang Yu , Wei Zhu-Ying , Bai Chun-Ling , Li Guang-Peng 
Published: July 2016

AbstractDuring the process of embryonic development in mammals, epigenetic modifications must be erased and reconstructed. In particular, the trimethylation of histone 3 lysine 27 (H3K27me3) is associated with gene-specific transcriptional repression and contributes to the maintenance of the pluripotent embryos. In this study, we determined that the global levels of the H3K27me3 marker were elevated in MII oocyte chromatin and decrease to minimal levels at the 8-cell and morula stages. When the blastocyst hatched, H3K27me3 was re-established in the inner cell mass. We also determined that H3K27me3-specific demethylases, UTX and JMJD3, were observed at high transcript and protein levels in mouse preimplantation embryos. In the activated oocytes, when the H3K27me3 disappeared at the 8-cell stage, the UTX (but not JMJD3) protein levels were undetectable. Using RNA interference, we suppressed UTX and JMJD3 gene expression in the embryos and determined that the functions of UTX and JMJD3 were complementary. When JMJD3 levels were decreased by RNA interference, the embryo development rate and quality were improved, but the knockdown of UTX produced the opposite results. Understanding the epigenetic mechanisms controlling preimplantation development is critical to comprehending the basis of embryonic development and to devise methods and approaches to treat infertility.
Methotrexate Nanoparticles Prepared with Codendrimer from Polyamidoamine (PAMAM) and Oligoethylene Glycols (OEG) Dendrons: Antitumor Efficacy in Vitro and in Vivo
Authors: Zhao Yanna , Guo Yifei , Li Ran , Wang Ting , Han Meihua , Zhu Chunyan , Wang Xiangtao 
Published: July 2016

AbstractThe novel methotrexate-loaded nanoparticles (MTX/PGD NPs) prepared with amphiphilic codendrimer PGD from polyamidoamine and oligothylene glycol dendrons were obtained via antisolvent precipitation method augmented by ultrasonication. Based on the excellent hydrophility of PGD, the drug-loaded nanoparticles could be investigated easily with the high drug-loading content (~85.2%, w/w). The MTX/PGD NPs possessed spherical morphology, nanoscaled particle size (approximately 182.4 nm) and narrow particle size distribution. Release of MTX from MTX/PGD NPs showed a sustained release manner and completed within 48 h. Hemolytic evaluation indicated MTX/PGD NPs presented good blood compatibility and the cytotoxicity of nanoparticles against breast cancer cells in vitro, biodistribution in tumor tissue and antitumor efficacy in vivo were enhanced significantly compared to MTX injection. According to the higher drug-loading content, enhanced antitumor efficacy and appropriate particle size, MTX/PGD NPs as the drug delivery systems could have potential application for cancer chemotherapy in clinic.
The effect of statins on prostate cancer recurrence and mortality after definitive therapy: a systematic review and meta-analysis
Authors: Tan Ping , Wei Shiyou , Yang Lu , Tang Zhuang , Cao Dehong , Liu Liangren , Lei Junhao , Fan Yu , Gao Liang , Wei Qiang 
Published: July 2016

AbstractIn this work, we aim to further analyze the association of statins use with biochemical recurrence (BCR) of prostate cancer (PCa) and PCa-specific mortality after definitive therapy. A systematic literature search of PubMed, MEDLINE and EMBASE through Jul 2015 was conducted. Pooled Hazard ratio (HR) estimates with corresponding 95% confidence intervals (CIs) were calculated using random-effects model. STATA version 10 (Stata corporation, college station, TX) was employed to conduct all statistical analyses. A total of 22 and 8 studies contributed to the biochemical recurrence analysis and PCa-specific mortality, respectively. 13 trials were included for BCR-free survival analysis. The combined result showed statins users had lowered 12% BCR risk of PCa compared with non-users (HR = 0.88, 95%CI: 0.765–0.998) (p < 0.05). The association was null among the men who underwent radical prostatectomy as primary therapy (HR = 0.96, 95%CI: 0.83–1.09), while the improved outcomes had be seen among patients who received radiation therapy (HR = 0.67, 95%CI: 0.48–0.86). After excluding the patients undergoing ADT, participants did not benefit from statins use (HR = 0.94, 95%CI: 0.77–1.11). Meanwhile, long-term statins using did not alter recurrence risk. A lower risk of prostate cancer-specific mortality was observed among statins users (HR = 0.68, 95%CI: 0.56–0.80). There was a plausible trend towards increasing the BCR-free survival rate among statins users.
Metabolite profiling of Dioscorea (yam) species reveals underutilised biodiversity and renewable sources for high-value compounds
Authors: Price Elliott J. , Wilkin Paul , Sarasan Viswambharan , Fraser Paul D. 
Published: July 2016

AbstractYams (Dioscorea spp.) are a multispecies crop with production in over 50 countries generating ~50 MT of edible tubers annually. The long-term storage potential of these tubers is vital for food security in developing countries. Furthermore, many species are important sources of pharmaceutical precursors. Despite these attributes as staple food crops and sources of high-value chemicals, Dioscorea spp. remain largely neglected in comparison to other staple tuber crops of tropical agricultural systems such as cassava (Manihot esculenta) and sweet potato (Ipomoea batatas). To date, studies have focussed on the tubers or rhizomes of Dioscorea, neglecting the foliage as waste. In the present study metabolite profiling procedures, using GC-MS approaches, have been established to assess biochemical diversity across species. The robustness of the procedures was shown using material from the phylogenetic clades. The resultant data allowed separation of the genotypes into clades, species and morphological traits with a putative geographical origin. Additionally, we show the potential of foliage material as a renewable source of high-value compounds.
Causal Inference Based on the Analysis of Events of Relations for Non-stationary Variables
Authors: Yin Yu , Yao Dezhong 
Published: July 2016

AbstractThe main concept behind causality involves both statistical conditions and temporal relations. However, current approaches to causal inference, focusing on the probability vs. conditional probability contrast, are based on model functions or parametric estimation. These approaches are not appropriate when addressing non-stationary variables. In this work, we propose a causal inference approach based on the analysis of Events of Relations (CER). CER focuses on the temporal delay relation between cause and effect and a binomial test is established to determine whether an “event of relation” with a non-zero delay is significantly different from one with zero delay. Because CER avoids parameter estimation of non-stationary variables per se, the method can be applied to both stationary and non-stationary signals.
Delineation of six species of the primitive algal genus Glaucocystis based on in situ ultrastructural characteristics
Authors: Takahashi Toshiyuki , Nishida Tomoki , Tuji Akihiro , Saito Chieko , Matsuzaki Ryo , Sato Mayuko , Toyooka Kiminori , Yasuda Hidehiro , Nozaki Hisayoshi 
Published: July 2016

AbstractThe field of microbiology was established in the 17thcentury upon the discovery of microorganisms by Antonie van Leeuwenhoek using a single-lens microscope. Now, the detailed ultrastructures of microorganisms can be elucidatedin situusing three-dimensional electron microscopy. Since the availability of electron microscopy, the taxonomy of microscopic organisms has entered a new era. Here, we established a new taxonomic system of the primitive algal genusGlaucocystis(Glaucophyta) using a new-generation electron microscopic methodology: ultra-high-voltage electron microscopy (UHVEM) and field-emission scanning electron microscopy (FE-SEM). Various globally distributedGlaucocystisstrains were delineated into six species, based on differences inin situultrastructural features of the protoplast periphery under UHVEM tomography and in the mother cell wall by FE-SEM, as well as differences in the light microscopic characteristics and molecular phylogenetic results. The present work onGlaucocystisprovides a model case of new-generation taxonomy.
Zero-valent Fe confined mesoporous silica nanocarriers (Fe(0) @ MCM-41) for targeting experimental orthotopic glioma in rats
Authors: Shevtsov M. A. , Parr M. A. , Ryzhov V. A. , Zemtsova E. G. , Arbenin A. Yu , Ponomareva A. N. , Smirnov V. M. , Multhoff G. 
Published: July 2016

AbstractMesoporous silica nanoparticles (MSNs) impregnated with zero-valent Fe (Fe(0) @ MCM-41) represent an attractive nanocarrier system for drug delivery into tumor cells. The major goal of this work was to assess whether MSNs can penetrate the blood-brain barrier in a glioblastoma rat model. Synthesized MSNs nanomaterials were characterized by energy dispersive X-ray spectroscopy, measurements of X-ray diffraction, scanning electron microscopy and Mössbauer spectroscopy. For the detection of the MSNs by MR and for biodistribution studies MSNs were labeled with zero-valent Fe. Subsequent magnetometry and nonlinear-longitudinal-response-M2 (NLR-M2) measurements confirmed the MR negative contrast enhancement properties of the nanoparticles. After incubation of different tumor (C6 glioma, U87 glioma, K562 erythroleukemia, HeLa cervix carcinoma) and normal cells such as fibroblasts and peripheral blood mononuclear cells (PBMCs) MSNs rapidly get internalized into the cytosol. Intracellular residing MSNs result in an enhanced cytotoxicity as Fe(0) @ MCM-41 promote the reactive oxygen species production. MRI and histological studies indicated an accumulation of intravenously injected Fe(0) @ MCM-41 MSNs in orthotopic C6 glioma model. Biodistribution studies with measurements of second harmonic of magnetization demonstrated an increased and dose-dependent retention of MSNs in tumor tissues. Taken together, this study demonstrates that MSNs can enter the blood-brain barrier and accumulate in tumorous tissues.
EcoTILLING revealed SNPs in GhSus genes that are associated with fiber- and seed-related traits in upland cotton
Authors: Zeng Yan-Da , Sun Jun-Ling , Bu Su-Hong , Deng Kang-Sheng , Tao Tao , Zhang Yuan-Ming , Zhang Tian-Zhen , Du Xiong-Ming , Zhou Bao-Liang 
Published: July 2016

AbstractCotton is the most important textile crop in the world due to its cellulose-enriched fibers. Sucrose synthase genes (Sus) play pivotal roles in cotton fiber and seed development. To mine and pyramid more favorable alleles for cotton molecular breeding, single nucleotide polymorphisms (SNPs) of GhSus family genes were investigated across 277 upland cotton accessions by EcoTILLING. As a result, a total of 24 SNPs in the amplified regions of eight GhSus genes were identified. These SNPs were significantly associated with at least one fiber- or seed-related trait measured in Nanjing, Anyang and Kuche in 2007–2009. Four main-effect quantitative trait nucleotides (QTNs) and five epistatic QTNs, with 0.76–3.56% of phenotypic variances explained by each QTN (PVE), were found to be associated with yield-related traits; six epistatic QTNs, with the 0.43–3.48% PVE, were found to be associated with fiber quality-related traits; and one main-effect QTN and one epistatic QTN, with the PVE of 1.96% and 2.53%, were found to be associated with seed oil content and protein content, respectively. Therefore, this study provides new information for molecular breeding in cotton.
Associations between Nine Polymorphisms in EXO1 and Cancer Susceptibility: A Systematic Review and Meta-Analysis of 39 Case-control Studies
Authors: Zhang Meng , Zhao Duran , Yan Cunye , Zhang Li , Liang Chaozhao 
Published: July 2016

AbstractAn increasing number of studies have highlighted the potential link between EXO1 polymorphisms and cancer risk, although no consensus has yet been obtained. Thus, we aimed to obtain a thorough and current assessment of EXO1 polymorphisms and cancer susceptibility by performing a meta-analysis. A comprehensive literature retrieval was performed on PubMed, EMbase, Web of Science and Wanfang databases. The odds ratio (OR) and 95% confidence interval (CI) were applied to assess the results. Finally, 39 case-control studies of the nine EXO1 polymorphisms that involved 21,651 cases and 21,348 controls met our inclusion criteria. The pooled analysis indicated that the rs1047840 polymorphism conferred a significantly increased susceptibility to cancer in an allelic model. Similarly, the rs3754093, rs1776177, rs9350, rs10802996, rs1635498, rs1776148 and rs851797 polymorphisms were also associated with an increased susceptibility to cancer in an allelic model, respectively, while no significant association was identified for rs1635517 polymorphism. For the rs1047840 polymorphism, in an ethnicity subgroup analysis, a significantly increased susceptibility to cancer for Asians was identified in all the genetic models and for Caucasians in an allelic model. Our findings provide the evidence that the rs1047840, rs9350, rs10802996, rs1635498, rs1776148, rs1776177, rs3754093 and rs851797 polymorphisms may act as risk factors for cancer.
A proposed experimental diagnosing of specular Andreev reflection using the spin orbit interaction
Authors: Yang Yanling , Zhao Bing , Zhang Ziyu , Bai Chunxu , Xu Xiaoguang , Jiang Yong 
Published: July 2016

AbstractBased on the Dirac-Bogoliubov-de Gennes equation, we theoretically investigate the chirality-resolved transport properties through a superconducting heterojunction in the presence of both the Rashba spin orbit interaction (RSOI) and the Dresselhaus spin orbit interaction (DSOI). Our results show that, if only the RSOI is present, the chirality-resolved Andreev tunneling conductance can be enhanced in the superconducting gap, while it always shows a suppression effect for the case of the DSOI alone. In contrast to the similar dependence of the specular Andreev zero bias tunneling conductance on the SOI, the retro-Andreev zero bias tunneling conductance exhibit the distinct dependence on the RSOI and the DSOI. Moreover, the zero-bias tunneling conductances for the retro-Andreev reflection (RAR) and the specular Andreev reflection (SAR) also show a qualitative difference with respect to the barrier parameters. When the RSOI and the DSOI are finite, three orders of magnitude enhancement of specular Andreev tunneling conductance is revealed. Furthermore, by analyzing the balanced SOI case, we find that the RAR is in favor of a parabolic dispersion, but a linear dispersion is highly desired for the SAR. These results shed light on the diagnosing of the SAR in graphene when subjected to both kinds of SOI.
STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection
Authors: Queval Christophe J. , Song Ok-Ryul , Deboosère Nathalie , Delorme Vincent , Debrie Anne-Sophie , Iantomasi Raffaella , Veyron-Churlet Romain , Jouny Samuel , Redhage Keely , Deloison Gaspard , Baulard Alain , Chamaillard Mathias , Locht Camille , Brodin Priscille 
Published: July 2016

AbstractMycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. tuberculosis replication is not fully understood. Here we reinvestigate the role of STAT3 specifically inside human macrophages shortly after M. tuberculosis uptake. We first show that STAT3 activation is mediated by IL-10 and occurs in M. tuberculosis infected cells as well as in bystander non-colonized cells. STAT3 activation results in the inhibition of IL-6, TNF-α, IFN-γ and MIP-1β. We further demonstrate that STAT3 represses iNOS expression and NO synthesis. Accordingly, the inhibition of STAT3 is detrimental for M. tuberculosis intracellular replication. Our study thus points out STAT3 as a key host factor for M. tuberculosis intracellular establishment in the early stages of macrophage infection.