bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer
Authors: Hou Qianshan , Li Chunzhen , Chong Yuhui , Yin Haofeng , Guo Yuchen , Yang Lanjie , Li Tianliang , Yin Shulei 
Published: October 2024

BackgroundNatural killer (NK) cells play crucial roles in mediating anti-cancer activity in breast cancer (BRCA). However, the potential of NK cell-related molecules in predicting BRCA outcomes and guiding personalized therapy remains largely unexplored. This study focused on developing a prognostic and therapeutic prediction model for BRCA by incorporating NK cell-related genes.MethodsThe data analyzed primarily originated from the TCGA and GEO databases. The prognostic role of NK cells was evaluated, and marker genes of NK cells were identified via single-cell analysis. Module genes closely associated with immunotherapy resistance were identified by bulk transcriptome-based weighted correlation network analysis (WGCNA). Following taking intersection and LASSO regression, NK-related genes (NKRGs) relevant to BRCA prognosis were screened, and the NK-related prognostic signature was subsequently constructed. Analyses were further expanded to clinicopathological relevance, GSEA, tumor microenvironment (TME) analysis, immune function, immunotherapy responsiveness, and chemotherapeutics. Key NKRGs were screened by machine learning and validated by spatial transcriptomics (ST) and immunohistochemistry (IHC).ResultsTumor-infiltrating NK cells are a favorable prognostic factor in BRCA. By combining scRNA-seq and bulk transcriptomic analyses, we identified 7 NK-related prognostic NKRGs (CCL5, EFHD2, KLRB1, C1S, SOCS3, IRF1, and CCND2) and developed an NK-related risk scoring (NKRS) system. The prognostic reliability of NKRS was verified through survival and clinical relevance analyses across multiple cohorts. NKRS also demonstrated robust predictive power in various aspects, including TME landscape, immune functions, immunotherapy responses, and chemotherapeutic sensitivity. Additionally, KLRB1 and CCND2 emerged as key prognostic NKRGs identified through machine learning and external validation, with their expression correlation with NK cells confirmed in BRCA specimens by ST and IHC.ConclusionsWe developed a novel NK-related gene signature that has proven valuable for evaluating prognosis and treatment response in BRCA, expecting to advance precision medicine of BRCA.
Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma
Authors: Pan Xiaoxia , Qian Hongyan , Sun Zhouna , Yi Qiong , Liu Ying , Lan Gangzhi , Chen Jia , Wang Gaoren 
Published: October 2024

BackgroundRadiotherapy resistance is an important reason for high mortality in lung cancer patients, but the mechanism is still unclear. Dysregulation of cell proliferation and death plays a crucial role in the onset and progression of lung adenocarcinoma (LUAD). In recent times, a novel form of cellular demise called disulfidptosis, has attracted increasing attention. However, it is unclear whether the radiation-related disulfidptosis genes have prognostic role in LUAD.MethodsA complete suite of bioinformatics tools was used to analyze the expression and prognostic significance of radiation-related disulfidptosis genes. Afterward, we investigated the predictive significance of the risk signature in tumor microenvironments (TME), somatic mutations, and immunotherapies. In addition, we conducted a series of experiments to verify the expression of differentially expressed radiotherapy related disulfidptosis genes (DERRDGs) in vitro.ResultsA total of 88 DERRDGs were found. We constructed and validated a novel prognostic model based on PRELP, FGFBP1, CIITA and COL5A1. The enrichment analysis showed the DERRDG affected tumor prognosis by influencing tumor microenvironments (TME) and immunotherapy. And we constructed nomogram to promote clinical application. In addition, q-PCR confirmed the significant differences in the expression of prognostic genes between A549 irradiation-resistance cell and A549. Finally, western-blot, IHC staining, and small interference experiment suggested that PRELP may be a potential biomarker for radiotherapy resistance, whose low expression was associated with poor outcomes in LUAD patients.ConclusionThis study reveals the signature and possible underlying mechanisms of DERRDGs in LUAD and discovered the key gene PRELP, which helps to identify new prognostic biomarkers and provides a basis for future research.
Ultrasound analysis of different forms of hemolytic uremic syndrome in children
Authors: Rink Lydia , Finkelberg Ilja , Kreuzer Martin , Schipper Lukas , Pape Lars , Cetiner Metin 
Published: October 2024

BackgroundHemolytic uremic syndrome (HUS) is the most common cause of acute kidney injury in children. It is mainly caused by Shiga toxin-producing enterohemorrhagicEscherichia coli(EHEC; STEC-HUS) and is more rarely caused by uncontrolled complement activation (cHUS). Renal replacement therapy is frequently required and kidney function recovers in the majority of patients. Ultrasound (US) is the preferred imaging modality for the evaluation of any renal failure. The aim of this study is the evaluation of US diagnostics in both HUS types at disease onset and in the course of the disease.Materials and methodsClinical, laboratory, and US data from the digital patient records of children admitted as inpatients with a diagnosis of HUS were recruited for a monocentric, retrospective analysis. STEC-HUS and cHUS were diagnosed when, in addition to the laboratory constellation, EHEC infection and complement system activation were verified, respectively. US examinations were performed by pediatricians with certified pediatric US experience.ResultsIn total, 30 children with STEC-HUS (13/25 male; median age of disease onset 2.9 years; most prevalent EHEC serotype was O157) and cHUS (2/5 male; median age of disease onset 5.4 years; 3/5 with proven pathogenic variation) were included. Renal replacement therapy proportions were comparable in the STEC-HUS and cHUS patients (64% vs. 60%). The resistance index (RI) was elevated at disease onset in the patients with STEC-HUS and cHUS (0.88 ± 0.10 vs. 0.77 ± 0.04,p = 0.13) and was similar in the STEC-HUS subcohorts divided based on dialysis requirement (yes: 0.86 ± 0.1; no: 0.88 ± 0.1;p = 0.74). Total kidney size at disease onset displayed a positive correlation with dialysis duration (R = 0.53,p = 0.02) and was elevated in both HUS types (177% ± 56 and 167% ± 53). It was significantly higher in the STEC-HUS subcohort which required dialysis (200.7% vs. 145%,p &lt; .029), and a regressor kidney size threshold value of 141% was indicated in the receiver operating characteristic analysis. A classification model using both US parameters sequentially might be of clinical use for predicting the need for dialysis in patients with STEC-HUS. The US parameters normalized over time.ConclusionThe US parameters of RI and total kidney size are valuable for the assessment of HUS at disease onset and during therapy, and may be helpful in the assessment of whether dialysis is required in patients with STEC-HUS.
Neurodevelopmental outcome in children between one and five years after persistent pulmonary hypertension of term and near-term newborns
Authors: Atlan Laetitia , Berthomieu Lionel , Karsenty Caroline , Gascoin Géraldine , Arnaud Catherine , Breinig Sophie 
Published: October 2024

BackgroundPersistent pulmonary hypertension of the newborn (PPHN) is a serious condition that affects 1–2 per 1,000 newborns. Scientific data report the existence of neurological developmental abnormalities between 10 and 30%, but the description of these disorders linked with this situation of cerebral hypoxia and haemodynamic failure remains poorly documented.ObjectiveThe main goal of this study was to describe the prevalence of neuro-psychomotor developmental disorders in children aged between one and five years old who have been hospitalised at birth in a neonatal intensive care unit for the management of PPHN.MethodsAll of the newborns ≥34 weeks of gestational age (WGA) with PPHN, treated with inhaled nitric oxide in our neonatal intensive care unit between January 2015 and December 2019 were retrospectively enrolled. An ASQ-3 standardised questionnaire, adapted to the appropriate age (12, 24, 36, 48 and 60 months) was performed by the parents.ResultsFifty-five children (81% of answers) with a median age of 36 months (11–68), whose real age was close to the one of the questionnaire (12, 24, 36, 48 and 60 months), have been included in this study. There was 47% of pathological score [borderline: less than 1 standard deviation (SD) or suspect: less than 2SD] in at least one of the five studied domains, mainly in communication (25%) and individual and social skills (22%), despite a high overall score of 250 [220; 285] out of 300 that improved with age.ConclusionThis study showed a significant prevalence of neuro-psychomotor developmental disorders which justifies making more accessible a prolonged and adapted follow-up for early and multidisciplinary screening and management of these children with PPHN history. Larger cohorts are needed to better explore long term outcome of these vulnerable term neonates.
SPAG6 overexpression decreases the pro-apoptotic effect of daunorubicin in acute myeloid leukemia cells through the ROS/JNK MAPK axis in a GSTP1-dependent manner
Authors: Luo Jie , Ding Li , Pan Shirui , Luo Jing , Zhao Haiqiu , Yin Jiaxiu , Su Rong , Zhang Jiamin , Liu Lin 
Published: October 2024

IntroductionAs a malignant hematological disease, the incidence of acute myeloid leukemia (AML) has exhibited an upward trend in recent years. Nevertheless, certain limitations persist in the treatment of AML. Sperm-associated antigen 6 (SPAG6) has been implicated in the onset and progression of various human cancers, with its expression levels significantly elevated in AML. Consequently, we undertook a series of experiments to investigate the role and underlying mechanisms of SPAG6 in AML cell lines.MethodsIn the in vitro experiments of this study, DEPs and GO and KEGG enrichment analysis subsequent to SPAG6 down-regulation were detected by TMT. CCK8 was employed to determine cell viability. The levels of apoptosis and ROS were measured by flow cytometry. In the in vivo experiments, a xenografted tumor model was constructed, and the expression of SPAG6 and GSTP1 in tumor tissues was detected by IHC.ResultsUltimately, our findings indicated that over-expression of SPAG6 promoted cell growth and decreased reactive oxygen species (ROS) and malondialdehyde levels. Furthermore, SPAG6 knockdown was found to diminish mitochondrial membrane potential and facilitate cell apoptosis. In vivo, SPAG6 could also promote tumor growth, suggesting that SPAG6 may serve as a pro-tumor factor. In addition, daunorubicin (DNR) may cause oxidative stress and initiate apoptosis, resulting in oxidative damage to AML cells. However, the overexpression of SPAG6 may attenuate the efficacy of DNR. This was due to SPAG6 promoted GSTP1 expression, thereby reducing ROS levels. Simultaneously, the elevation of GSTP1 and JNK complex may reduce the expression of p-JNK and inhibit the activation of JNK pathway, which might inhibit cell apoptosis.DiscussionIn conclusion, our experiments suggested that upregulated SPAG6 might mitigate the pro-apoptotic effects of DNR through ROS/JNK MAPK axis in a GSTP1-dependent manner.
Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats
Authors: Albrahim Tarfa , Alangry Raghad , Alotaibi Raghad , Almandil Leen , Alburikan Sara , Aloudah Hisham S. , Alahmed Mohammed , Alonazi Mona 
Published: October 2024

BackgroundOsteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current study is to examine the reported osteo-preservative effects of exercise and/or fasting in the Ramadan fasting model (RFM) in ovariectomized (OVX) rats.MethodsThe experimental intervention started 1 month following the ovariectomy procedure and consisted of five 15-min exercise sessions per week at 18–25 m/min and/or an approximately 13-h fast from sunrise to sunset (6:00 AM–19:00 PM). Serum bone metabolism biomarker levels were measured, and mineral concentrations in femoral ashed bones and digested serum were determined. Additionally, serum bone alkaline phosphatase (b-ALP), parathyroid hormone, osteocalcin, calcitonin, and vitamin D3 concentrations were measured using the competitive enzyme immunoassay technique.ResultsCalcium, magnesium, and phosphorus showed a notable decrease in mineral concentration among OVX rat femurs compared with the combination group (OVX + RFM + E) and control groups. In addition, homeostasis of serum concentrations of calcium, magnesium, and phosphorus was observed to increase in the OVX + RFM + E group rather than in the OVX group without intervention when compared with a control group. Furthermore, fasting and exercise, either alone or concurrently with ovariectomy, induced a non-significant elevation in osteocalcin, parathyroid hormone, and vitamin D3, whereas b-ALP and calcitonin increased significantly compared with those in control rats.ConclusionThe combination of the Ramadan fasting model and moderate intensity exercises among OVX rats manifested advantageous effects in bone biomarkers compared with OVX rats without intervention. This could be recommended as a lifestyle modification that is protective against osteoporosis, especially in the context of depleted estrogen hormone after menopause.
Threats to the security of banking activities in modern Russian practice
Authors: Panina D. 
Published: October 2024

Among other sectors of the Russian economy, the banking sector is one of the most dynamically developing. Consequently, its resistance to various shocks and possible threats will be one of the central points of support for the economic security of the state. Modern statistics show an increase in various types of incidents in the financial sector. Regular monitoring and detailed analysis of fraudulent schemes will minimize the possibility of criminal manipulation, thereby significantly reducing the number of risks and the amount of financial losses.The presented article provides a structural and geographical analysis of incidents in the financial sector recorded in 2023, and a sketch of the modern landscape of possible threats is compiled. The optimal approach to the formation of information and economic security of personal and financial data, in particular, and banking activities in general, involves comprehensive protection measures both from the point of view of the individual user and from the position of representatives of the banking community and the regulator.
Risk of Postoperative Hypermagnesemia in Cardiopulmonary Bypass–Assisted Cardiovascular Surgery
Authors: Ghaddar Malak , Hatab Taha , El-Kaakour Adel , Tamim Hani , Makki Maha , El-Halabi Tasnim , Rifaii Khalid , Sfeir Pierre , Hoteit Mayssaa , Koubar Sahar H. 
Published: October 2024

Key Points

The incidence of hypermagnesemia after cardiovascular surgeries using cardiopulmonary bypass is 53.2%. Most patients had mild hypermagnesemia.Cardioplegic solutions with higher magnesium content and lower eGFR were independently linked to an increased risk of hypermagnesemia.Large multicenter studies should explore the effects of hypermagnesemia on clinical outcomes in patients with advanced CKD undergoing cardiopulmonary bypass surgeries.

Background
Magnesium (Mg) administration is a common practice in cardiovascular surgeries using cardiopulmonary bypass (CPB). However, concerns persist regarding the risk of hypermagnesemia, particularly in patients with kidney dysfunction. The aims of this study were to determine the incidence of postoperative hypermagnesemia in CPB-assisted cardiovascular surgeries and identify the associated risk factors.

Methods
This was a retrospective cohort study conducted at a tertiary medical center. Data from adult patients undergoing open-heart surgery using CPB between 2018 and 2020 were analyzed. Sociodemographic, perioperative, and clinical variables were collected from electronic medical records. Logistic regression was used to identify independent risk factors of hypermagnesemia.

Results
Of the 278 patients analyzed, 53.2% developed postoperative hypermagnesemia (Mg ≥2.5 mg/dl). Mild hypermagnesemia (Mg, 2.5–3.9 mg/dl) was most common with no significant effect on clinical outcomes observed. Patients with hypermagnesemia were older, with higher comorbidity burdens and lower baseline eGFR. Cardioplegic solutions with higher Mg content and lower baseline eGFR were independently associated with hypermagnesemia (odds ratio [OR], 64.3; 95% confidence interval [CI], 12.9 to 501.1 and OR, 1.3; 95% CI, 1.1 to 1.5, respectively). Notably, ultrafiltration on CPB was associated with low risk of hypermagnesemia (OR, 0.4; 95% CI, 0.1 to 1.0; P value, 0.048).

Conclusions
This study highlights the importance of mindful Mg supplementation strategies in those with advanced kidney disease. Future large-scale prospective multicenter studies should validate these findings and explore the extended effects of hypermagnesemia on clinical outcomes in patients with advanced CKD undergoing CPB surgeries.
КЛИНИЧЕСКИЙ СЛУЧАЙ COVID19 В ГЕСТАЦИОННОМ ПЕРИОДЕ
Authors: М.М. Тусупбекова , Л.М. Стабаева , Г.Н. Иманбаева , Р.Ж. Ныгызбаева , Б.А. Ибраимов 
Published: October 2024

Введение. Женщины, которые беременны, подвергаются повышенному риску тяжелого заболевания COVID-19. Беременные женщины с SARS-CoV-2, вирусу, вызывающему COVID-19, также с большей вероятностью могут подвергаться преждевременным родам и повышенному риску возникновения осложнений. Во время пандемии на ваше здоровье и здоровье вашего ребенка могут повлиять стресс, беспокойство и депрессия. Учитывая все выше перечисленное, необходимо проведение полного обследования здоровья беременных, не зависимо от клинических проявлений. Описание случая. Нами представлен клинический случай роженицы Г., 1992г., с клиническими проявлениями ОРВИ в период пандемии, но при этом не проведены рутинные методы диагностики COVID-19. Заключение. Плацента представляет собой хорошо перфузируемый орган, разделяющий кровообращение матери и плода. Инфекция SARS-CoV-2 может усиливать воспалительный и окислительный стресс в плаценте, тем самым ставя под угрозу, как течение беременности, так и вызывая аномальное развитие плаценты, что влияет на исходы для матери и плода.
Introduction. Women, who are pregnant are at increased risk of severe COVID-19 disease. Pregnant women with SARS-CoV-2, the virus that causes COVID-19, are also more likely to be exposed to premature birth and an increased risk of complications. During a pandemic, stress, anxiety and depression can affect your health and the health of your child. Considering all of the above, it is necessary to conduct a full examination of the health of pregnant women, regardless of clinical manifestations. Case description. We present a clinical case of a woman in labor in 1992, with clinical manifestations of ARVI during the pandemic, but routine methods of diagnosis of COVID-19 have not been carried out. Conclusion. The placenta is a well-perfused organ that separates the blood circulation of the mother and fetus. SARS-CoV-2 infection can increase inflammatory and oxidative stress in the placenta, thereby jeopardizing both the course of pregnancy and causing abnormal placental development, which affects outcomes for the mother and fetus.
Кіріспе. Жүкті әйелдерге ауырcovid-19 қаупі жоғары. SARS-CoV-2 бар жүкті әйелдер, COVID-19 тудыратын вирус, сонымен қатар, мерзімінен бұрын босануға және асқыну қаупінің жоғарылауына әкелуі мүмкін. Пандемия кезінде стресс, мазасыздық және депрессия Сіздің денсаулығыңыз бен балаңыздың денсаулығына әсер етуі мүмкін. Жоғарыда айтылғандардың бәрін ескере отырып, клиникалық көріністерге қарамастан, жүкті әйелдердің денсаулығын толық тексеру қажет. Жағдайды сипаттау. Біз пандемия кезінде ЖРВИ-нің клиникалық көріністерімен 1992 жылы босанған әйелдің клиникалық жағдайын ұсындық, бірақCOVID-19 диагнозының күнделікті әдістері жүргізілмеген. Қорытынды. Плацента-бұл ана мен ұрықтың қан айналымын бөлетін жақсы перфузияланғанмүше. SARS-CoV-2 инфекциясы плацентадағы қабыну және тотығу стрессін күшейте алады, осылайша жүктіліктің өтуіне қауіп төндіреді және ана мен ұрықтың нәтижелеріне әсер ететін плацентаның қалыпты дамуын тудырады.
RECONSTRUCTION OF BILATERAL DOLICHOARTERIOPATHY OF THE INTERNAL CAROTID ARTERY: CASE REPORT
Authors: Ye.K. Dogalbaev , A.B. Fursov , T.A. Sultanaliev , I.N. Sagandykov , S.S. Suleymenov , K.B. Іlbekova 
Published: October 2024

Currently, in patients with bilateral dolichoarteriopathy of the internal carotid artery (DICA), the optimal tactics of surgical treatment has not been determined. We report the case of a 59-year-old woman with a history of transient ischemic attack. The patient was examined, ultrasound duplex scanning (USDS) of the brachiocephalic arteries revealed a bilateral DICA with an acceleration of the linear blood flow velocity (LBFV) at the site of angulation, up to 1.7 m/s on the right, up to 1.6 m/s on the left. Computed tomography (CT) of intra- and extracranial arteries with contrast confirmed the presence of a DICA. The patient was operated stage-by-stage on both sides. USDS of the carotid arteries (CA) and CT of the extra- and intracranial arteries with contrast were performed 6 months after the second operation. Both showed normal results. The result of surgical treatment of bilateral DICA showed that the right treatment tactics can lead to regression of cerebral and focal neurological symptoms.
В настоящее время у больных с двусторонней патологической извитостью (ПИ) внутренней сонной артерии (ВСА) не определена оптимальная тактика хирургического лечения. Мы сообщаем о клиническом случае 59-летней женщины с транзиторной ишемической атакой в анамнезе. При обследовании у больной, на ультразвуковом дуплексном сканировании (УЗДС) брахиоцефальных артерий (БЦА) выявлена двусторонняя ПИ ВСА с ускорением линейной скорости кровотока (ЛСК) в месте ангуляции, до 1,7 м/с справа, до 1,6 м /с слева. Компьютерная томография (КТ) интра- и экстракраниальных артерий с контрастированием подтвердила наличие ПИ ВСА. Больная оперирована поэтапно с обеих сторон. Через 6 месяцев после повторной операции выполнили УЗДС сонных артерий и КТ экстра- и интракраниальных артерий с контрастированием. Оба показали нормальные результаты. Результат хирургического лечения двусторонней ПИ ВСА показал, что правильная лечебная тактика может привести к регрессу общемозговой и очаговой неврологической симптоматики.
Қазіргі уақытта ішкі ұйқы артериясының (ІҮА) екі жақты патологиялық бұралуы (ПБ) бар науқастарда хирургиялық емдеудің оңтайлы тактикасы анықталмаған. Біз өтпелі ишемиялық ұстамамен ауыратын 59 жастағы әйелдің клиникалық жағдайын хабарлаймыз. Науқасты тексерген кезде брахиоцефалиялық артерияларды ультрадыбыстық дуплексті сканерлеуде (УДДС) ІҮА екі жақты ПБ бұрыштық жерде қан ағымының сызықтық жылдамдығының үдеуімен, оң жақта 1,7 м/с дейін, сол жақта 1,6 м/с дейін. Контрастты интра- және экстракраниальды артериялардың компьютерлік томографиясы (КТ) ІҮА ПБ болуын растады. Науқасқа екі жаққа да кезең-кезеңімен ота жасалды. Қайта операциядан кейін 6 ай өткен соң ұйқы артерияларының УДДС және контрастпен интра- және экстракраниальды артериялардың КТ жасалды. Екеуі де қалыпты нәтиже көрсетті. ІҮА екі жақты ПБ хирургиялық емдеу нәтижесі дұрыс емдеу тактикасы церебральды және ошақты неврологиялық симптомдардың регрессиясына әкелуі мүмкін екенін көрсетті.