bluuebunny/crossref_metadata_2025_split huggingface dataset:
HHV-8-unrelated primary effusion-like lymphoma associated with clonal loss of inherited chromosomally-integrated human herpesvirus-6A from the telomere of chromosome 19q
Authors: Zhang Enjie , Cotton Victoria E. , Hidalgo-Bravo Alberto , Huang Yan , J. Bell Adam , F. Jarrett Ruth , Wilkie Gavin S. , Davison Andrew J. , P. Nacheva Ellie , Siebert Reiner , Majid Aneela , Kelpanides Inga , Jayne Sandrine , Dyer Martin J. , Royle Nicola J. 
Published: March 2016

AbstractPrimary effusion lymphomas (PEL) are associated with human herpesvirus-8 (HHV-8) and usually occur in immunocompromised individuals. However, there are numerous reports of HHV-8-unrelated PEL-like lymphomas with unknown aetiology. Here we characterize an HHV-8-unrelated PEL-like lymphoma in an elderly woman who was negative for human immunodeficiency viruses 1 and 2 and hepatitis B and C. The woman was, however, a carrier of an inherited-chromosomally-integrated human herpesvirus-6A (iciHHV-6A) genome in one 19q telomere. The iciHHV-6A genome was complete in blood DNA, encoding a full set of protein-coding genes. Interestingly, the entire iciHHV-6A genome was absent from the HHV-8-unrelated-PEL-like lymphoma cells despite retention of both copies of chromosome 19. The somatic loss of the 19q-iciHHV-6A genome occurred very early during lymphoma development and we propose it occurred via telomere-loop formation and excision to release a circular viral genome that was subsequently lost. Whether release of the HHV-6A genome from the telomere contributed to lymphomagenesis, or was coincidental, remains unclear but this event may have deregulated the expression of HHV-6A or 19q genes or else disrupted telomere function. To establish the frequency and importance of iciHHV-6 loss from telomeres, the HHV-6 copy number should be assessed in tumours that arise in iciHHV-6 carriers.
Subcortical cytoskeleton periodicity throughout the nervous system
Authors: D’Este Elisa , Kamin Dirk , Velte Caroline , Göttfert Fabian , Simons Mikael , Hell Stefan W. 
Published: March 2016

AbstractSuperresolution fluorescence microscopy recently revealed a ~190 nm periodic cytoskeleton lattice consisting of actin, spectrin and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is a structural feature of all neurons and how it is modified when axons are ensheathed by myelin forming glial cells is not known. Here, STED nanoscopy is used to demonstrate that this structure is a commonplace of virtually all neuron types in vitro. To check how the subcortical meshwork is modified during myelination, we studied sciatic nerve fibers from adult mice. Periodicity of both actin and spectrin was uncovered at the internodes, indicating no substantial differences between unmyelinated and myelinated axons. Remarkably, the actin/spectrin pattern was also detected in glial cells such as cultured oligodendrocyte precursor cells. Altogether our work shows that the periodic subcortical cytoskeletal meshwork is a fundamental characteristic of cells in the nervous system and is not a distinctive feature of neurons, as previously thought.
Non-coding RNA LINC00473 mediates decidualization of human endometrial stromal cells in response to cAMP signaling
Authors: Liang Xiao-Huan , Deng Wen-Bo , Liu Yue-Fang , Liang Yu-Xiang , Fan Zong-Min , Gu Xiao-Wei , Liu Ji-Long , Sha Ai-Guo , Diao Hong-Lu , Yang Zeng-Ming 
Published: March 2016

AbstractDecidualization is an essential step in the establishment of pregnancy. However, the functional contributions of long intergenic noncoding RNAs (LincRNAs) to decidualization have not been explored. To explore the regulation and role of LincRNAs during human decidualization, human endometrial stromal cells (HESCs) are induced to undergo in vitro decidualization by treating with estradiol-17β, db-cAMP and medroxyprogesterone acetate. LINC00473 (LINC473) expression is highly induced in HESCs after decidual stimulus. We found that cAMP-PKA pathway regulates the expression of LINC473 through IL-11-mediated STAT3 phosphorylation. RNA interference-mediated down-regulation of LINC473 inhibits in vitro decidualization. These results suggested that LINC473 might be functionally required for human decidualization. This is the first report demonstrating the presence of LincRNA during human decidualization.
Temozolomide-perillyl alcohol conjugate induced reactive oxygen species accumulation contributes to its cytotoxicity against non-small cell lung cancer
Authors: Song Xingguo , Xie Li , Wang Xingwu , Zeng Qian , Chen Thomas C. , Wang Weijun , Song Xianrang 
Published: March 2016

AbstractTemozolomide-perillyl alcohol conjugate (TMZ − POH), a novel temozolomide analog, was reported to play a cytotoxic role in triple-negative breast cancer and TMZ-resistant gliomas. In a current study we had demonstrated how TMZ − POH also exhibited its cytotoxicity against non-small cell lung cancer (NSCLC), the most common type of lung cancer, as evidence from cell/tumor proliferation inhibition, G2/M arrest, DNA damage and mitochondrial apoptosis. Importantly, TMZ − POH’s cytotoxicity is closely related to reactive oxygen species (ROS) accumulation because it can be reversed by two ROS scavengers, catalase (CAT) and N-acetyl-L-cysteine (NAC). TMZ − POH induces mitochondrial transmembrane potential (MTP) decrease and ROS accumulation, in turn activates mitogen-activated protein kinase (MAPKs) signaling and mitochondrial apoptosis, and then exerts its cytotoxicity, thus proposing TMZ − POH as a potential therapeutic candidate for NSCLC.
Erionite-Na upon heating: dehydration dynamics and exchangeable cations mobility
Authors: Ballirano Paolo , Pacella Alessandro 
Published: March 2016

AbstractErionite is a fibrous zeolite significantly more tumorigenic than crocidolite asbestos upon inhalation. In recent years, several papers have been published aimed at characterizing from the crystal-chemical point of view erionite fibres. As their toxicity has been ascribed to Fe acquired within the human body, studies aimed at characterizing the iron topochemistry have also been published, suggesting a possible important role played by the ionic exchange properties and cations mobility of this zeolite on developing carcinogenicity. Here we report the analysis results of the thermal behaviour of erionite-Na, which has been found to deviate significantly from that of erionite-K. This result is in contrast with the current scientific view that differences in weighted ionic potential, Si/Al ratio and size of exchangeable cations result in significantly different thermal behaviours, all those parameters being nearly identical or very similar in both species. The different mobility of the extraframework cations observed in erionite samples with dissimilar chemistry is of particular interest within the frame of the hypothesis that their biological activity could depend, apart from surface interactions, also on bulk effects.
Covalent immobilization of metal–organic frameworks onto the surface of nylon—a new approach to the functionalization and coloration of textiles
Authors: Yu Ming , Li Wanxin , Wang Ziqiang , Zhang Bowu , Ma Hongjuan , Li Linfan , Li Jingye 
Published: March 2016

AbstractThe prevention of refractory organic pollution caused by conventional dyeing and the development of new fabrics with various functions are two issues to be solved urgently in the field of textile fabrication. Here, we report a new environmentally friendly route for the simultaneous coloration and functionalization of textiles by the covalent immobilization of a metal–organic framework, Cr-based MIL-101(Cr), onto the surfaces of nylon fabrics by co-graft polymerization with 2-hydroxyethyl acrylate initiated by γ-ray irradiation. The Cr(III) clusters color the nylon fabric, and the color intensity varies with the MIL-101 content, providing a “green” textile coloration method that is different from conventional dyeing processes. An X-ray diffraction (XRD) analysis shows that the nanoporous structure of the original MIL-101 particles is retained during radiation-induced graft polymerization. Numerous nanopores are introduced onto the surface of the nylon fabric, which demonstrated better sustained-release-of-aroma performance versus pristine nylon fabric in tests. The modified fabrics exhibit laundering durability, with MIL-101 nanoparticles intact on the nylon surface after 30 h of dry cleaning.
Genetic scores of smoking behaviour in a Chinese population
Authors: Yang Shanshan , He Yao , Wang Jianhua , Wang Yiyan , Wu Lei , Zeng Jing , Liu Miao , Zhang Di , Jiang Bin , Li Xiaoying 
Published: March 2016

AbstractThis study sought to structure a genetic score for smoking behaviour in a Chinese population. Single-nucleotide polymorphisms (SNPs) from genome-wide association studies (GWAS) were evaluated in a community-representative sample (N = 3,553) of Beijing, China. The candidate SNPs were tested in four genetic models (dominance model, recessive model, heterogeneous codominant model and additive model) and 7 SNPs were selected to structure a genetic score. A total of 3,553 participants (1,477 males and 2,076 females) completed the survey. Using the unweighted score, we found that participants with a high genetic score had a 34% higher risk of trying smoking and a 43% higher risk of SI at ≤18 years of age after adjusting for age, gender, education, occupation, ethnicity, body mass index (BMI) and sports activity time. The unweighted genetic scores were chosen to best extrapolate and understand these results. Importantly, genetic score was significantly associated with smoking behaviour (smoking status and SI at ≤18 years of age). These results have the potential to guide relevant health education for individuals with high genetic scores and promote the process of smoking control to improve the health of the population.
Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans
Authors: Li Wen-Ru , Shi Qing-Shan , Dai Huan-Qin , Liang Qing , Xie Xiao-Bao , Huang Xiao-Mo , Zhao Guang-Ze , Zhang Li-Xin 
Published: March 2016

AbstractThe antifungal activity, kinetics and molecular mechanism of action of garlic oil againstCandida albicanswere investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane ofC. albicansas well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed that garlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase and heat shock proteins. This is suggestive of aC. albicansstress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions ofC. albicans.
Immobilizing photogenerated electrons from graphitic carbon nitride for an improved visible-light photocatalytic activity
Authors: Sun Han , Cao Yue , Feng Leiyu , Chen Yinguang 
Published: March 2016

AbstractReducing the recombination probability of photogenerated electrons and holes is pivotal in enhancing the photocatalytic ability of graphitic carbon nitride (g-C3N4). Speeding the departure of photogenerated electrons is the most commonly used method of achieving this. To the best of our knowledge, there is no report on suppressing the recombination of photogenerated electron–hole pairs by immobilizing the electrons with ester functional groups. Here, for the first time the mesoporous g-C3N4 (mpg-C3N4) was integrated with polymethyl methacrylate, a polymer abundant in ester groups, which showed a photocatalytic activity unexpectedly higher than that of the original mpg-C3N4 under visible-light irradiation. Experimental observations, along with theoretical calculations, clarified that the impressive photocatalytic ability of the as-modified mpg-C3N4 was mainly derived from the immobilization of photogenerated electrons via an electron-gripping effect imposed by the ester groups in the polymethyl methacrylate. This novel strategy might also be applied in improving the photocatalytic performance of other semiconductors.
Novel Slide-Ring Material/Natural Rubber Composites with High Damping Property
Authors: Wang Wencai , Zhao Detao , Yang Jingna , Nishi Toshio , Ito Kohzo , Zhao Xiuying , Zhang Liqun 
Published: March 2016

AbstractA novel class of polymers called “slide-ring” (SR) materials with slideable junctions were used for high damping composites for the first time. The SR acts as the high damping phase dispersed in the natural rubber (NR) matrix, and epoxidized natural rubber (ENR) acts as the compatibilizer. The morphological, structural, and mechanical properties of the composites were investigated by atomic force microscope (AFM), transmission electron microscope (TEM), dynamic mechanical thermal analyzer (DMTA), rubber processing analyzer (RPA), and tensile tester. AFM and TEM results showed that the SR phase was uniformly dispersed in the composites, in a small size that is a function of ENR. DMTA and RPA results showed that the damping factor of the composites is much higher than that of NR, especially at room temperatures. Stretch hysteresis was used to study the energy dissipation of the composites at large strains. The results showed that SR and ENR can significantly improve the dissipation efficiency at strains lower than 200% strain. Wide-angle X-ray diffraction was used to study the strain-induced crystallization of the composites. The results indicated that the impact of the SR on the crystallization of NR is mitigated by the insulating effect of ENR.
Soil gross nitrogen transformations along the Northeast China Transect (NECT) and their response to simulated rainfall events
Authors: Zhang Jin-bo , Wang Liang , Zhao Wei , Hu Hui-feng , Feng Xiao-juan , Müller Christoph , Cai Zu-cong 
Published: March 2016

AbstractClimate changes are predicted to increase extreme rainfall events in semiarid and arid region in Northern Hemisphere. Nutrient cycles will be affected by the precipitation changes but so far only very little is known how soil N transformations may respond. Here we investigated gross soil N transformation rates and their response to simulated rainfall events across Northeast China Transect (NECT). The results showed that gross N mineralisation rate, nitrification rate and nitrification to mineralisation ratio significantly increased as the humidity index decreased along NECT, resulting in NO3− as the predominant inorganic N form. These characteristics could increase the risk of NO3− losses but at the same time reduce the risk of N losses via volatilization in the semiarid and arid region. The soil-plant ecosystems have developed effective N conservation strategies in the long term with respect to the prevailing climate in arid region. However, compared to humid soils more dramatic changes of soil N transformation rates are likely to occur in arid soils, after sudden soil moisture increases. Soil N conservation mechanisms in arid regions were drastically affected when the heavy rainfall frequently occurred. Arid ecosystems are expected to be more vulnerable than humid ecosystems in response to extreme rainfall events.
Using the sliding‐mode control approach for analysis and design of the boost inverter
Authors: Flores‐Bahamonde Freddy , Valderrama‐Blavi Hugo , Bosque‐Moncusi Josep Maria , García Germain , Martínez‐Salamero Luis 
Published: March 2016

A cascade control strategy is employed in the boost inverter to generate a sinusoidal signal at grid frequency with very low distortion. The internal loop of the cascade employs a switching surface based on the difference of the inductor currents to induce sliding motions in the power stage. The outer loop in turn establishes the reference of the internal loop and ensures the tracking of an external sinusoidal signal at 50 Hz. The reported approach is analytical and is based on the equivalent control method. The root locus of the capacitor voltages at the equilibrium point of the inner loop is obtained assuming a constant value of the loop reference. In the particular case of a zero reference, sinusoidal variations at grid frequency are superposed to the corresponding equilibrium point and the resulting ideal dynamics are linearised yielding the control to output transfer function of the system. A proportional‐integral (PI) compensator is designed for both large bandwidth and small phase error. Experimental results in a 500 W prototype are in perfect agreement with the analytical predictions.
Numerical Integration of Temperature‐Dependent Functions in Bioenergetics Models to Avoid Overestimation of Fish Growth
Authors: Holsman Kirstin , Danner Eric 
Published: March 2016

AbstractThe Wisconsin bioenergetics model is widely used to evaluate the effects of environmental conditions, trophic interactions, and human‐mediated alterations to physical and trophodynamic processes on the growth and survival of individual fish species. In particular, bioenergetics models are increasingly applied to evaluate conditions that vary on subdaily time steps, such as vertical migrations that influence thermal experience and fluvial alterations that increase diurnal temperature variability. However, because the algorithms that describe the relationship between temperature and physiological rates are often nonlinear, using inputs of daily mean temperatures can result in underestimation or overestimation of growth and energetic demand. We used simulations of daily and subdaily models of Chinook Salmon Oncorhynchus tshawytscha as an example to demonstrate that the nonlinear, temperature‐dependent algorithms for consumption and respiration induce large differences in growth between constant‐ and fluctuating‐temperature conditions (despite the same mean daily temperature); these differences increase with high diurnal variability and as temperatures approach the thermal optimum for the species. To correct for model bias in growth, we propose an integrated temperature‐scaling algorithm that allows application of the daily model to systems where daily temperatures exhibit considerable subdaily variation. This approach can also be used for any bioenergetics model that includes nonlinear, temperature‐dependent algorithms and should be considered when modeled temperatures approach inflection points in nonlinear relationships.Received February 11, 2015; accepted September 8, 2015
Using Radiotelemetry to Compare the Initial Behavior and Mortality of Hatchery‐Reared and Wild Juvenile Florida Bass
Authors: Thompson Brandon C. , Porak Wesley F. , Leone Erin H. , Allen Micheal S. 
Published: March 2016

AbstractStocking evaluations for pellet‐reared, advanced‐fingerling Largemouth Bass Micropterus salmoides often result in low contribution and survival, but little information exists on their survival and behavior soon after release. This study investigated the behavior and mortality of pellet‐reared, advanced‐fingerling (90–120 mm TL) Florida Bass M. floridanus immediately after they were stocked in Lake Carlton, Florida. A radiotelemetry experiment was conducted to compare initial movement, habitat use, growth, and mortality between stocked hatchery and wild fish. In year 1, we inserted radio transmitters into 50 hatchery fish; in year 2, we tagged 30 hatchery fish and 20 wild fish. All mortality we observed for hatchery fish (from both years) resulted from predation by fish and birds, and most occurred in the first 7 d after tagging. When compared directly with tagged wild fish (n = 20), tagged hatchery fish (n = 30) exhibited greater movement (75 and 124 m/d, respectively), greater proportion of locations offshore (8% and 23%, respectively), slower growth (1.73% and 0.41% of their body weight gained per day, respectively), and higher predation (47% and 0%, respectively). Results of this study suggest that pellet‐reared, hatchery Florida Bass suffer more predation than wild bass of similar size initially following their release into the lake. Causes of predation could include less‐developed antipredator skills and inefficiency in foraging observed through greater movement, as well as the use of offshore habitat and slower growth. We recommend research that investigates acclimating advanced‐fingerling Florida Bass to predators and live prey before release to determine whether initial predation can be reduced after stocking.Received May 29, 2015; accepted December 4, 2015
Development of Similar Broth Microdilution Methods to Determine the Antimicrobial Susceptibility of Flavobacterium columnare and F. psychrophilum
Authors: Gieseker Charles M. , Crosby Tina C. , Mayer Tamara D. , Bodeis Sonya M. , Stine Cynthia B. 
Published: March 2016

Abstract
Flavobacterium columnare and F. psychrophilum are major fish pathogens that cause diseases that may require antimicrobial therapy. Choice of appropriate treatment is dependent upon determining the antimicrobial susceptibility of isolates. Therefore we optimized methods for broth microdilution testing of F. columnare and F. psychrophilum to facilitate standardizing an antimicrobial susceptibility test. We developed adaptations to make reproducible broth inoculums and confirmed the proper incubation time and media composition. We tested the stability of potential quality-control bacteria and compared test results between different operators. Log phase occurred at 48 h for F. columnare and 72–96 h for F. psychrophilum, confirming the test should be incubated at 28°C for approximately 48 h and at 18°C for approximately 96 h, respectively. The most consistent susceptibility results were achieved with plain, 4-g/L, dilute Mueller–Hinton broth supplemented with dilute calcium and magnesium. Supplementing the broth with horse serum did not improve growth. The quality-control strains, Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658, yielded stable minimal inhibitory concentrations (MIC) against all seven antimicrobials tested after 30 passes at 28°C and 15 passes at 18°C. In comparison tests, most MICs of the isolates agreed 100% within one drug dilution for ampicillin, florfenicol, and oxytetracycline. The agreement was lower with the ormetoprim–sulfdimethoxine combination, but there was at least 75% agreement for all but one isolate. These experiments have provided methods to help standardize antimicrobial susceptibility testing of these nutritionally fastidious aquatic bacteria.
Received June 24, 2015; accepted October 2, 2015.