bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Proteomic Analysis of the Mitochondria-associated ER Membrane (MAM) in a Long-term Type 2 Diabetic Rodent Model
Authors: Ma Jacey Hongjie , Shen Shichen , Wang Joshua J. , He Zhanwen , Poon Amanda , Li Jun , Qu Jun , Zhang Sarah X. 
Published: May 2017

AbstractThe mitochondria-associated ER membrane (MAM) plays a critical role in cellular energetics and calcium homeostasis; however, how MAM is affected under diabetic condition remains elusive. This study presented a comprehensive proteome profiling of isolated brain MAM from long-term type 2 diabetic mice vs. non-diabetic controls. MAM protein was extracted efficiently by a surfactant-aided precipitation/on-pellet digestion (SOD) method, and MAM proteome was quantified by an ion-current-based MS1 method combined with nanoLC-MS/MS. A total of 1,313 non-redundant proteins of MAM were identified, among which 144 proteins were found significantly altered by diabetes. In-depth IPA analysis identified multiple disease-relevant signaling pathways associated with the MAM proteome changes in diabetes, most significantly the unfolded protein response (UPR), p53, hypoxia-related transcription factors, and methyl CpG binding protein 2. Using immunofluorescence labeling we confirmed the activation of three UPR branches and increased ERp29 and calreticulin in diabetic retinas. Moreover, we found GRP75, a key MAM tethering protein, was drastically reduced by long-term diabetes. In vitro, acute high glucose treatment reduces ER-mitochondrial contact in retinal endothelial cells. This study provides first insight into the significant alterations in MAM proteome associated with activation of the UPR in diabetes, which may serve as novel benchmarks for the future studies of diabetic complications.
Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair
Authors: Li Bo , Lee Carol , Filler Tali , Hock Alison , Wu Richard You , Li Qi , Chen Shigang , Koike Yuhki , Ip Wan , Chi Lijun , Zani-Ruttenstock Elke , Määttänen Pekka , Gonska Tanja , Delgado-Olguin Paul , Zani Augusto , Sherman Philip M. , Pierro Agostino 
Published: May 2017

AbstractMaternal separation (MS) in neonates can lead to intestinal injury. MS in neonatal mice disrupts mucosal morphology, induces colonic inflammation and increases trans-cellular permeability. Several studies indicate that intestinal epithelial stem cells are capable of initiating gut repair in a variety of injury models but have not been reported in MS. The pathophysiology of MS-induced gut injury and subsequent repair remains unclear, but communication between the brain and gut contribute to MS-induced colonic injury. Corticotropin-releasing hormone (CRH) is one of the mediators involved in the brain–gut axis response to MS-induced damage. We investigated the roles of the CRH receptors, CRHR1 and CRHR2, in MS-induced intestinal injury and subsequent repair. To distinguish their specific roles in mucosal injury, we selectively blocked CRHR1 and CRHR2 with pharmacological antagonists. Our results show that in response to MS, CRHR1 mediates gut injury by promoting intestinal inflammation, increasing gut permeability, altering intestinal morphology, and modulating the intestinal microbiota. In contrast, CRHR2 activates intestinal stem cells and is important for gut repair. Thus, selectively blocking CRHR1 and promoting CRHR2 activity could prevent the development of intestinal injuries and enhance repair in the neonatal period when there is increased risk of intestinal injury such as necrotizing enterocolitis.
Prevalence of Auditory Problems in Children With Feeding and Swallowing Disorders
Authors: Rawool Vishakha Waman 
Published: May 2017

PurposeAlthough an interdisciplinary approach is recommended for assessment and management of feeding or swallowing difficulties, audiologists are not always included in the interdisciplinary team. The purpose of this study is to report the prevalence of middle ear and hearing problems in children with feeding and swallowing disorders and to compare this prevalence with that in typical children.MethodA total of 103 children were included in the study: 44 children with feeding and swallowing disorders and 59 children without any such disorders. Audiological examinations included case-history information, visualization of the ear canals through otoscopy, middle ear evaluation through tympanometry, and hearing screenings using an audiometer.ResultsThe odds of excessive cerumen (p= .0000, small effect size), middle ear dysfunction (p= .0148, small effect size), and hearing screening failure (p= .0000, large effect size) were 22.14%, 2.97%, and 13.5% higher, respectively, in children with feeding and swallowing disorders compared with typically developing children.ConclusionThe significantly higher prevalence of hearing problems in children with feeding and swallowing disorders compared with typically developing children suggests that inclusion of an audiologist on the interdisciplinary team is likely to improve overall interventional outcomes for children with feeding and swallowing disorders.
Response to de Wit et al., 2016, “Characteristics of Auditory Processing Disorders: A Systematic Review”
Authors: Moncrieff Deborah 
Published: May 2017

Purpose

This letter to the editor is in response to a review by de Wit et al. (2016), “Characteristics of Auditory Processing Disorders: A Systematic Review,” published in April 2016 by
Journal of Speech, Language, and Hearing Research.

Conclusion
The author argues that the conclusions in the de Wit et al. (2016) review are unfortunate in light of advances made in the clinical diagnosis and treatment of bottom-up auditory processing disorders in children.
High‐speed wireless infrared uplink scheme for airplane passengers’ communications
Authors: Tagliaferri D. , Capsoni C. 
Published: May 2017

Hybrid visible light communication/infrared communication (VLC/IRC) systems are becoming the leading way for supplying high‐speed Internet connectivity to airplane passengers. A robust IR uplink transmission scheme is presented for in‐flight VLC/IRC systems which has the potential to support 10 Mbit/s transmission for each user in the cabin with uncoded bit error rate ≤10−6 under the assumption of line‐of‐sight communication scenario, also in the presence of misalignments between transmitter and receiver due to random movements of the passengers.
Accuracy of Blood Loss Measurement during Cesarean Delivery
Authors: Doctorvaladan Sahar , Jelks Andrea , Hsieh Eric , Thurer Robert , Zakowski Mark , Lagrew David 
Published: May 2017

Objective This study aims to compare the accuracy of visual, quantitative gravimetric, and colorimetric methods used to determine blood loss during cesarean delivery procedures employing a hemoglobin extraction assay as the reference standard.
Study Design In 50 patients having cesarean deliveries blood loss determined by assays of hemoglobin content on surgical sponges and in suction canisters was compared with obstetricians' visual estimates, a quantitative gravimetric method, and the blood loss determined by a novel colorimetric system. Agreement between the reference assay and other measures was evaluated by the Bland–Altman method.
Results Compared with the blood loss measured by the reference assay (470 ± 296 mL), the colorimetric system (572 ± 334 mL) was more accurate than either visual estimation (928 ± 261 mL) or gravimetric measurement (822 ± 489 mL). The correlation between the assay method and the colorimetric system was more predictive (standardized coefficient = 0.951, adjusted R2 = 0.902) than either visual estimation (standardized coefficient = 0.700, adjusted R2 = 00.479) or the gravimetric determination (standardized coefficient = 0.564, adjusted R2 = 0.304).
Conclusion During cesarean delivery, measuring blood loss using colorimetric image analysis is superior to visual estimation and a gravimetric method. Implementation of colorimetric analysis may enhance the ability of management protocols to improve clinical outcomes.
Jumbocups in der Revision
Authors: von Roth Philipp , Wassilew Georgi 
Published: May 2017

ZusammenfassungExtragroße zementfreie hemisphärische Pressfit-Pfannen werden als Jumbocups bezeichnet und wurden insbesondere in der letzten Dekade aufgrund ihrer einfachen Implantationstechnik häufig bei großen knöchernen Defekten eingesetzt. Durch ihre Größe füllen die Komponenten die azetabulären Defekte jedoch nur aus und tragen somit nicht zur Regeneration des knöchernen Lagers bei. Aufgrund dessen sind gerade bei diesem Implantattyp die Langzeitüberlebensraten von großem Interesse. Der vorliegende Beitrag diskutiert die zu diesem Thema vorliegende, relevante Literatur und geht auf die Operationstechnik und implantatspezifische Besonderheiten von Jumbocups ein. Jumbocups zeigen ein gutes Langzeitüberleben wobei dennoch die aseptische Lockerung die häufigste Revisionsursache und die Luxation die häufigste Komplikation darstellt. Durch die Entwicklung von alternativen meist modularen Revisionssystemen haben Jumbocups in der täglichen Praxis an Bedeutung verloren. Sie sind aber definitiv eine Benchmark für Neuentwicklungen im Bereich der Hüftendoprothetik.
Metabolic Syndrome and Aggressive Prostate Cancer at Initial Diagnosis
Authors: Di Francesco Simona , Tenaglia Raffaele 
Published: May 2017

AbstractLinks between metabolic syndrome and prostate cancer after androgen deprivation therapy are emerging. The aim of the research was to investigate the association of metabolic syndrome and aggressive prostate malignancy, at initial diagnosis, without the influence of hormonal treatment. Retrospective analysis of 133 patients with prostate tumor diagnosis between 2007 and 2009 was conducted. Patients with prostate cancer were subdivided in 2 groups according to Gleason score: Gleason score≥7 as high-grade prostate tumor (Group 1) and <7 (Group 2) as low-grade prostate tumor. Metabolic syndrome was defined according to International Diabetes Federation and the American Heart Association/National Heart, Lung, and Blood Institute definition. Metabolic syndrome was significantly associated with aggressive prostate cancer (OR 1.87, p<0.05) and a reduced risk of low-grade prostate cancer (OR 0.53, p<0.05) at initial diagnosis, without the influence of endocrine therapy. In our study, patients with metabolic syndrome were more likely to present with more aggressive prostate carcinoma vs. patients without metabolic syndrome. Further research should elucidate these relations in larger samples to confirm these associations and to stabilize future prevention and therapeutic strategies.
The wisdom of crowds for visual search
Authors: Juni Mordechai Z. , Eckstein Miguel P. 
Published: May 2017

SignificanceSimple majority voting is a widespread, effective mechanism to exploit the wisdom of crowds. We explored scenarios where, from decision to decision, a varying minority of group members often has increased information relative to the majority of the group. We show how this happens for visual search with large image data and how the resulting pooling benefits are greater than previously thought based on simpler perceptual tasks. Furthermore, we show how simple majority voting obtains inferior benefits for such scenarios relative to averaging people’s confidences. These findings could apply to life-critical medical and geospatial imaging decisions that require searching large data volumes and, more generally, to any decision-making task for which the minority of group members with high expertise varies across decisions.
Entropic forces drive self-organization and membrane fusion by SNARE proteins
Authors: Mostafavi Hakhamanesh , Thiyagarajan Sathish , Stratton Benjamin S. , Karatekin Erdem , Warner Jason M. , Rothman James E. , O’Shaughnessy Ben 
Published: May 2017

Significance
Secretion of neurotransmitters and hormones depends on membrane fusion, accomplished by a cellular fusion machinery whose core components are SNARE proteins. Several vesicle-associated v-SNAREs form SNAREpin complexes with target membrane-associated t-SNAREs, but how they cooperate to catalyze fusion is unknown. Using highly coarse-grained simulations, we found that steric interactions spontaneously organized SNAREpins into circular clusters, expanded the clusters, and forced membranes together. These are entropic effects, reminiscent of cooperative transitions in solutions of rod-like molecules. The rate of fusion was controlled by the number of SNAREs, because more SNAREs generated greater entropic forces. Given ∼70 v-SNAREs available per synaptic vesicle, entropic cooperativity among SNAREpins may underlie the sub-millisecond timescale of neurotransmitter release.
Dynamic microtubules regulate cellular contractility during T-cell activation
Authors: Hui King Lam , Upadhyaya Arpita 
Published: May 2017

Significance
T-cell activation is an essential event in the adaptive immune response to fight against infections. T cells were recently shown to be mechanosensitive, and to exert forces actively on the opposing antigen-presenting cell during activation. However, the molecular basis of force generation by T cells is poorly understood. Here, we report a molecular mechanism that involves the T-cell microtubule (MT) and actomyosin cytoskeletons, which interact through Rho GTPase activity. Our study highlights the role played by the MT cytoskeleton in regulating actomyosin-generated forces, which play a key role in T-cell activation.
Structural basis for spumavirus GAG tethering to chromatin
Authors: Lesbats Paul , Serrao Erik , Maskell Daniel P. , Pye Valerie E. , O’Reilly Nicola , Lindemann Dirk , Engelman Alan N. , Cherepanov Peter 
Published: May 2017

Significance
Spumaviruses are being developed as vectors for gene-therapy applications, but how these retroviruses select genomic locations for integration remains unknown. Here we use X-ray crystallography to visualize the interaction between the spumaviral GAG protein and a nucleosome. We show that this interaction is essential for the observed distribution of spumavirus integration sites in various human cell types. Thus, despite stark differences in the mechanistic details of spumavirus and orthoretrovirus replication strategies, both retroviral subfamilies depend on their structural proteins to locate optimal integration sites.
Shelterin components mediate genome reorganization in response to replication stress
Authors: Mizuguchi Takeshi , Taneja Nitika , Matsuda Emiko , Belton Jon-Matthew , FitzGerald Peter , Dekker Job , Grewal Shiv I. S. 
Published: May 2017

Significance
Genome organization affects many critical nuclear functions. Notably, the nuclear periphery has emerged as a specialized compartment for the regulation of transcription, replication, and DNA damage repair activities. Here, we find that cells carrying a mutation in the broadly distributed DNA-binding protein Sap1 experience replication stress and genome instability and undergo a genome reorganization featuring new contacts between chromosome arms and telomeres. These prominent new interactions are mediated by the Taz1–Shelterin telomere protection complex and include specific chromosome arm regions containing replication origins bound by Taz1. Our findings uncover an unexpected role for Shelterin in mediating genome reorganization in cells undergoing replication stress.
Globalism and cultural tensions
Authors: Li Mo , Albakry Mohammed 
Published: May 2017

Abstract
Based on a corpus of 200 articles from the People’s Daily and the People’s Daily Overseas Edition collected from 2010 to 2012, we examined the representation of English, applying framing theory (Chong & Druckman, 2007). The results indicate four dominant frames shared by both newspapers: exclusion/oppression, warfare/protection, yardstick/benchmark, and bridge/needs. Both papers perceive the English language as a resource while constructing a Chinese identity fundamentally in competition with a Western identity reinforced by the English language. However, while both papers project the image of China as a unified, benign country proud of its linguistic and cultural heritage, the Overseas Edition seems more conscious in representing China as a motherland in need of protection from the threatening socio-cultural force of English. The article seeks to contribute to the growing body of research on language and identity in China, English and globalization, and the perception of English in the expanding circle.
Discursive and pragmatic functions of Latin em
Authors: Gómez Luis Unceta 
Published: May 2017

Abstract
The Latin particle em, mainly attested in Archaic Latin, is the apocopated form of eme, the imperative of the verb emere ‘to take, acquire, purchase’. The traditional understanding of this element has been that of a secondary interjection. This interpretation, however, presents a number of problems that can be traced to the lack of a pragmatic perspective. In a corpus as old as the comedies of Plautus (c. 254–184 bc), em seems completely grammaticalized and paired with procedural meanings. This paper offers an in-depth analysis of the various discursive and pragmatic functions of em, mainly addressee-oriented and used to regulate the communicative exchange, and compares this Latin unit with similar ones in certain modern languages. In this way, this study not only helps us better understand the procedural meanings of this particular unit, but also offers a diachronic perspective of pragmaticalization phenomena.
Constituent order in directives with stative verbs in Latin
Authors: Cabrillana Concepción 
Published: May 2017

Abstract
This paper considers constituent ordering in monovalent and bivalent constructions of two Latin stative verbs – sum ‘to be’ and fio ‘to become’ – when they appear in directives, assessing the degree of speaker-addressee interaction. We use Risselada’s classification of the different subtypes of directives based on the extent to which compliance with what the speaker expresses is obligatory. The findings reveal that, given (i) the difference in the number of arguments and in semantic content of monovalent and bivalent structures, and (ii) the incidence of modality (declarative/directive), the order of constituents different in each case. Furthermore, tendencies in constituent patterns are more uniform when expressions are more prototypically directive, although not in a wholly scalar way. Hence, the peculiarities or deviations observed require specific explanations, such as the structure of “world-creation”, the kind of proposal made, or the existence of expressions with different degrees of lexicalization.
Res Gestae Divi Augusti
Authors: de la Villa Jesús 
Published: May 2017

Abstract
This paper analyzes the word order of the Greek text of the Res Gestae Divi Augusti and compares it with the word order of the original Latin version. This comparison shows that the predominant word order in the Greek text largely coincides with the basic patterns previously proposed for literary texts. Second, that there is a high degree of coincidence between the word order patterns of the Latin and the Greek versions of this text. However, there are discrepancies in a number of cases. These discrepancies are analyzed in detail and some explanations are proposed for them.
Calling translation to the bar
Authors: Orlando Daniele 
Published: May 2017

Abstract
This paper proposes a comparative analysis of the translation errors made by prospective legal translation trainees, with a special focus on the (mis)use of legal terminology and phraseology. The investigation relies on the data produced and collected within a wider empirical study on the translation problems faced by a cohort of translation graduates with no specialisation in legal translation on the one hand, and a cohort of linguistically-skilled lawyers with no translation-related qualifications on the other, who translated the same criminal law document from English into Italian. The translation errors made by the two cohorts have been classified on the basis of the categories proposed by Mossop (2014) and assessed following the severity scale devised by Vollmar (2001). The Translation Quality Index (cf. Schiaffino and Zearo 2006) thus obtained has allowed for the ranking of the participants in the five quality levels identified for legal translation by Prieto Ramos (2014). The findings of the quantitative and qualitative analyses of errors are also traced back to the participants’ translation process by triangulating data from the different collection methods used within the empirical study, i.e. screen recording, keystroke logging and questionnaires, with particular reference to time and reference material use. The specific design of this investigation, which considers the participants’ prior education as additional variable, allows for the identification of a possible correlation between the different backgrounds of the translators and the quality of their translations, with general consequences on the conceptualisation of legal translation competence and effective training.
Use of Morphological and Physiological Indices to Characterize Life History Diversity in Juvenile Hatchery Winter‐Run Steelhead
Authors: Larsen Donald A. , Middleton Mollie A. , Dickey Jon T. , Gerstenberger Ryan S. , Brun Chris V. , Swanson Penny 
Published: May 2017

AbstractSteelhead Oncorhynchus mykiss exhibit significant life history plasticity, including variation in freshwater residence time, age of smoltification, and maturation that are influenced by exceeding specific thresholds in size or body energy stores. Fish that do not reach minimum thresholds for smoltification or exceed thresholds for maturation may “residualize” in freshwater. Understanding life history diversity is important in steelhead hatchery programs. The objective of this investigation was to characterize life history diversity in Hood River, Oregon, hatchery winter‐run steelhead prior to release. We used both morphological and physiological indices to assess degree of smoltification in both sexes and maturation in males. Smoltification was categorized by measuring size, visual smolt index, and gill Na+/K+‐ATPase activity. All females were determined to be immature. Male reproductive development required histological staging of testes. Other indices of maturation—including gonadosomatic index, plasma 11‐ketotestosterone, and pituitary fshb and lhb and testis ifg3 and amh gene expression—were also employed, with varying degrees of success. Five phenotypes were described: (1) immature male and female parr; (2) precociously mature male parr; (3) male smolts in early to mid‐spermatogenesis that are likely to spawn after <1 year in the ocean; (4) males that might have attempted maturation, but failed; and (5) immature male and female smolts that could mature after >1 year in the ocean. Over three brood years, we estimated that 3–4% of the population could potentially residualize due to failure to smolt or precocious male maturation, 1–12% could mature after <1 year in the ocean (one salt male), and 84–95% could mature after >1 year in the ocean (two to three salt males and females). Based on previous studies conducted in the Hood River basin, the residualism rate estimated for this hatchery population is lower than that of naturally rearing Hood River steelhead.Received November 2, 2016; accepted February 14, 2017 Published online May 11, 2017