bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine
Authors: Raeven René H. M. , Brummelman Jolanda , Pennings Jeroen L. A. , van der Maas Larissa , Tilstra Wichard , Helm Kina , van Riet Elly , Jiskoot Wim , van Els Cécile A. C. M. , Han Wanda G. H. , Kersten Gideon F. A. , Metz Bernard 
Published: December 2016

AbstractThe demand for improved pertussis vaccines is urgent due to the resurgence of whooping cough. A deeper understanding of the mode of action of pertussis vaccines is required to achieve this improvement. The vaccine-induced effects of a candidate outer membrane vesicle vaccine (omvPV) and a classical protective but reactogenic whole cell vaccine (wPV) were comprehensively compared in mice. The comparison revealed essential qualitative and quantitative differences with respect to immunogenicity and adverse effects for these vaccines. Both vaccines stimulated a mixed systemic Th1/Th2/Th17 response. Remarkably, omvPV evoked higher IgG levels, lower systemic pro-inflammatory cytokine responses and enhanced splenic gene expression than wPV. The omvPV-induced transcriptome revealed gene signatures of the IFN-signaling pathway, anti-inflammatory signatures that attenuate LPS responses, anti-inflammatory metabolic signatures, and IgG responses. Upon intranasal challenge, both immunized groups were equally efficient in clearingBordetella pertussisfrom the lungs. This study importantly shows that immunization with omvPV provides a milder inflammatory responses but with equal protection to bacterial colonization and induction of protective antibody and Th1/Th17 type immune responses compared to wPV. These results emphasize the potential of omvPV as a safe and effective next-generation pertussis vaccine.
IntegratedMRF: random forest-based framework for integrating prediction from different data types
Authors: Rahman Raziur , Otridge John , Pal Ranadip 
Published: December 2016

Abstract

Summary
IntegratedMRF is an open-source R implementation for integrating drug response predictions from various genomic characterizations using univariate or multivariate random forests that includes various options for error estimation techniques. The integrated framework was developed following superior performance of random forest based methods in NCI-DREAM drug sensitivity prediction challenge. The computational framework can be applied to estimate mean and confidence interval of drug response prediction errors based on ensemble approaches with various combinations of genetic and epigenetic characterizations as inputs. The multivariate random forest implementation included in the package incorporates the correlations between output responses in the modeling and has been shown to perform better than existing approaches when the drug responses are correlated. Detailed analysis of the provided features is included in the Supplementary Material.

Availability and Implementation
The framework has been implemented as a R package IntegratedMRF, which can be downloaded from https://cran.r-project.org/web/packages/IntegratedMRF/index.html, where further explanation of the package is available.

Supplementary information
Supplementary data are available at Bioinformatics online.
Effects of Cardiopulmonary Bypass on Renal Perfusion, Filtration, and Oxygenation in Patients Undergoing Cardiac Surgery
Authors: Lannemyr Lukas , Bragadottir Gudrun , Krumbholz Vitus , Redfors Bengt , Sellgren Johan , Ricksten Sven-Erik 
Published: December 2016

Abstract

Background
Acute kidney injury is a common complication after cardiac surgery with cardiopulmonary bypass. The authors evaluated the effects of normothermic cardiopulmonary bypass on renal blood flow, glomerular filtration rate, renal oxygen consumption, and renal oxygen supply/demand relationship, i.e., renal oxygenation (primary outcome) in patients undergoing cardiac surgery.

Methods
Eighteen patients with a normal preoperative serum creatinine undergoing cardiac surgery procedures with normothermic cardiopulmonary bypass (2.5 l · min−1 · m−2) were included after informed consent. Systemic and renal hemodynamic variables were measured by pulmonary artery and renal vein catheters before, during, and after cardiopulmonary bypass. Arterial and renal vein blood samples were taken for measurements of renal oxygen delivery and consumption. Renal oxygenation was estimated from the renal oxygen extraction. Urinary N-acetyl-β-d-glucosaminidase was measured before, during, and after cardiopulmonary bypass.

Results
Cardiopulmonary bypass induced a renal vasoconstriction and redistribution of blood flow away from the kidneys, which in combination with hemodilution decreased renal oxygen delivery by 20%, while glomerular filtration rate and renal oxygen consumption were unchanged. Thus, renal oxygen extraction increased by 39 to 45%, indicating a renal oxygen supply/demand mismatch during cardiopulmonary bypass. After weaning from cardiopulmonary bypass, renal oxygenation was further impaired due to hemodilution and an increase in renal oxygen consumption, accompanied by a seven-fold increase in the urinary N-acetyl-β-d-glucosaminidase/creatinine ratio.

Conclusions
Cardiopulmonary bypass impairs renal oxygenation due to renal vasoconstriction and hemodilution during and after cardiopulmonary bypass, accompanied by increased release of a tubular injury marker.
Activation of PKCα and PI3K Kinases in Hypertrophic and Nodular Port Wine Stain Lesions
Authors: Yin Rong , Gao Lin , Tan Wenbin , Guo Wei , Zhao Tao , Nelson Jhon Stuart , Wang Gang 
Published: December 2016

Abstract:
Port wine stain (PWS) is a congenital, progressive vascular malformation. Many patients with PWS develop hypertrophy and discrete nodularity during their adult life, but the mechanism(s) remain incompletely understood. In this study, we attempted to investigate activation status of PKCα, PI3K, PDPK1 and PLC-γ and protein levels of PP2A and DAG to explore their potential roles in the formation of hypertrophic and nodular PWS lesions. We found phosphorylated levels of PKCα, PI3K, PDPK1, and PLC-γ and protein levels of PP2A and DAG showed moderate increases in the endothelial cells of hypertrophic PWS as compared to the adjacent normal skin. These increases extended throughout the entire stroma of blood vessels in PWS nodules. Many proliferating cells, such as fibroblasts, also showed strong activation of PKCα, PI3K, PDPK1 and PLC-γ and upregulations of PP2A and DAG in nodular PWS lesions. Our data showed that there is aberrant activation of PKCα, PI3K, PDPK1 and PLC-γ and upregulation of PP2A and DAG mainly in endothelial cells in hypertrophic PWS areas, but presenting in the entire vasculatures and surrounding fibroblasts in PWS nodules. Our data suggest that both PKCα and PI3K signaling pathways contribute to the development of hypertrophy and nodularity in adult PWS.
Follicular Malignant Melanoma: A Rare Morphologic Variant of Melanoma. Report of a Case and Review of the Literature
Authors: Chapman Iona , Horton Sharon , Liu Wenhua 
Published: December 2016

Abstract:
Follicular malignant melanoma (FMM) is an exceedingly rare variant of melanoma. Only 8 cases have been reported in the literature thus far. Often bland on physical examination, FMM shows a striking histologic pattern, with atypical melanocytes sometimes consuming the entire follicle and extending into the dermis. Criteria have been suggested to help distinguish FMM from lentigo maligna melanoma; however, no clear criteria distinguish this cancer clinically. FMM cannot be distinguished from follicular metastases of melanoma based on histology, requiring close clinical correlation and detailed history taking on the part of the practitioner. In this publication, we present a case of FMM and a review of the literature. A 59-year-old man was incidentally found to have a small flesh-colored papule on the arm during a skin cancer screening. Biopsy revealed atypical melanocytes extending from the follicular infundibulum into the reticular dermis. Immunohistochemical staining confirmed the melanocytic origin of the neoplasm. The patient underwent wide local excision with 2-cm margins. Sentinel lymph node biopsy was negative for melanoma. We present this case to highlight an unusual type of melanoma and illustrate how it may be distinguished from other neoplasms.
A mouse model for chronic pain-induced increase in ethanol consumption
Authors: Butler Ryan K. , Knapp Darin J. , Ulici Veronica , Longobardi Lara , Loeser Richard F. , Breese George R. 
Published: December 2016

Abstract
Chronic pain conditions are often comorbid with alcohol abuse. “Self-medication” with alcohol introduces a host of problems associated with the abuse of alcohol which over time has the potential of exacerbating the painful condition. Despite the prevalence of chronic pain being associated with alcohol abuse, rodent models which mimic the comorbid conditions are lacking. In this study, we model osteoarthritis (OA) in C57BL/6J mice by surgically destabilizing the medial meniscus (DMM). Sham-operated mice served as controls. Thirteen weeks after surgery, DMM but not sham-operated mice exhibited pronounced incapacitance of the surgically manipulated hind limb compared with the nonsurgically manipulated hind limb. At this time, the mice were exposed to the 2-bottle ethanol choice, beginning with 2.5% with a gradual increasing to 20%. Compared with sham controls, DMM mice consumed more EtOH and preferred EtOH over water at the 20% EtOH concentration. Histological analysis verified that the DMM mice exhibited significant damage to the articular cartilage and osteophyte growth compared with sham controls and these measures of the severity of OA correlated with the amount of ethanol intake. Thus, the combination of the DMM model of OA with the enhanced two-bottle ethanol choice is a potential preclinical approach in mice by which the basis of the comorbid association of alcohol abuse and chronic pain conditions can be explored.
Who's your daddy?: paternal inheritance of metabolic disease risk
Authors: Isganaitis Elvira , Suehiro Harumi , Cardona Connie 
Published: December 2016

Purpose of review
Although the importance of optimizing mothers’ health prior to conception and during pregnancy is now well accepted, recent data also implicate health and nutritional status of fathers as contributors to chronic disease risk in their progeny. This brief review will highlight recent epidemiological and experimental studies linking paternal overnutrition, undernutrition, and other forms of stress, to metabolic disease in the offspring.

Recent findings
The past 2 years have brought tremendous insights into the mechanisms by which paternal exposures can contribute to disease susceptibility in the next generation. Recent data, both from humans and experimental models, demonstrate that paternal obesity and undernutrition result in epigenetic reprogramming of male germ cells, notably altered DNA methylation, histone retention, and expression of small noncoding RNAs and transfer RNA fragments. Novel mechanisms have also been identified, such as epididymal transport vesicles, seminal fluid hormones and metabolites, and a unique seminal fluid microbiome.

Summary
Paternal nutritional and other perturbations are linked to risk of metabolic disease and obesity in offspring. Germ cell-dependent mechanisms have recently been linked to these intergenerational effects. Nongenetic, paternal inheritance of chronic disease has important implications for public health, and may provide novel opportunities for multigenerational disease prevention.
Association Between Clinical Nurse Specialist’s Presence and Open Visitation in US Intensive Care Units
Authors: Suba Sukardi , Donesky DorAnne , Scruth Elizabeth Ann , Carrieri-Kohlman Virginia 
Published: December 2016

Background:
Current evidence shows that an open visitation policy can benefit patients and families during intensive care unit (ICU) stays. Therefore, an unrestricted visitation policy in the ICU has been proposed as part of patient-centered care.

Purpose:
The purpose of this secondary analysis is to explore the likelihood of an ICU with a clinical nurse specialist (CNS) having an open visitation policy when compared with an ICU without a CNS.

Design:
This is a secondary analysis of a survey conducted between 2008 and 2009, describing the ICU visitation practices in more than 600 hospitals across the United States.

Methods:
χ2 Analysis was performed comparing the presence of CNS in the ICU with visitation policies, with P < 0.05 considered statistically significant.

Results:
There were data from 347 hospitals used in the analysis, with 47 hospitals (13.5%) having open visitation policies. There were 108 hospitals (31%) that had a CNS present in the ICU; 14 of the hospitals (13%) had open visitation policies. No significant correlation was found between a CNS being present in the ICU and open visitation policy (odds ratio, 0.93; P = .83), regardless of hospital geographic regions.

Conclusions:
Although CNS presence was not associated with open visitation in this study, given the strong evidence to support the benefits to patients and the CNS role as a change agent in the hospital system, liberalization of visitation in the ICU is an area that could benefit from CNS advocacy.
Correlational Study of Diabetic Retinopathy and Hearing Loss
Authors: Ooley Caroline , Jun Weon , Le Kim , Kim Allen , Rock Nathan , Cardenal Molly , Kline Rebecca , Aldrich Drew , Hayes John 
Published: December 2016

ABSTRACT

Purpose
Our research goal was to complete a retrospective chart review to determine if there is a correlation between the level of diabetic retinopathy and diabetic neurosensory hearing loss.

Methods
A retrospective analysis of 175 Department of Veterans Affairs Computerized Patient Record System charts was completed at the VA Portland Health Care System. Subjects were classified by degree of diabetic retinopathy as follows: no diabetic retinopathy (n = 80), mild nonproliferative diabetic retinopathy (n = 51), moderate nonproliferative diabetic retinopathy (n = 25), and combined severe nonproliferative diabetic retinopathy and proliferative diabetic retinopathy (PDR) (n = 17). Degree of sensorineural hearing was collected for each ear. Additionally, measures of diabetic control, including hemoglobin A1C, and creatinine, were recorded.

Results
After controlling for diabetic control, as measured by HbA1C and creatinine, level of diabetic retinopathy was significantly associated with hearing loss severity in both ears (right ear, P = .018 and left ear, P = .007).

Conclusions
When adjusted to include diabetes control, the severity of diabetic retinopathy showed a correlation with degree of hearing loss at most levels. Because of this association, recommendation for hearing evaluations may be considered for those with mild, moderate, or severe nonproliferative or proliferative diabetic retinopathy.
Brief Report: CD14+ Enriched Peripheral Cells Secrete Cytokines Unique to HIV-Associated Neurocognitive Disorders
Authors: Agsalda-Garcia Melissa A. , Sithinamsuwan Pasiri , Valcour Victor G. , Chalermchai Thep , Tipsuk Somporn , Kuroda Jason , Nakamura Christie , Ananworanich Jintanat , Zhang Guangxiang , Schuetz Alexandra , Slike Bonnie M. , Shiramizu Bruce ,   on behalf of the SEARCH 011 Study Group
Published: December 2016

Abstract:Monocytes play a vital role in HIV-associated neurocognitive disorder (HAND), postulated to transport HIV into the brain and secrete pro-inflammatory cytokines. We analyzed cytokines released by cultured peripheral blood mononuclear cells enriched with the CD14+marker isolated from HIV-infected individuals with HAND and normal cognition (NC) in combination antiretroviral therapy naive and after 1 year on treatment. Interleukin-8 and monocyte chemoattractant protein-1 levels were higher in HAND compared with NC at baseline (P= 0.002 andP< 0.0001). These cytokines remained higher in HAND patients 1 year after combination antiretroviral therapy and were significant when NC patients who were initially HAND were excluded (P= 0.012 andP= 0.002). Both correlated with baseline CD14+peripheral blood mononuclear cell HIV DNA levels supporting the role of HIV DNA reservoir size and monocyte cytokines in HAND persistence.
Profiling information-seeking behaviour of distance learning students in Wawasan Open University
Authors: Vighnarajah  , Ali Hassan Farzanah , Abd Aziz Norhasni , Siew Lee Ooi 
Published: December 2016

Purpose
Wawasan Open University Library undertook a survey study, based on the Wilson’s revised model of information behaviour (1999), to profile information-seeking behaviour of their distance students in using library resources in their research and learning activities. The paper aims to discuss these issues.

Design/methodology/approach
Using random sampling method, samples were selected to ensure proper representation of the population across four schools of studies and five regional centres. A total of 550 questionnaires were sent to undergraduate and postgraduate students, and 435 completed questionnaires were returned with a success response rate of 79 per cent.

Findings
Findings indicated significant differences between undergraduate and postgraduate students in using the university’s MyDigital Library and physical library for their information needs. Findings also indicated significant difference between first-year and post-first-year students in using internet search engines as part of their information-seeking process.

Practical implications
Students improving on their information-seeking behaviour in the learning and research work paved way for them to better experience university vivacity and not isolate themselves from distance learning.

Originality/value
This paper acknowledges the importance of promoting good information-seeking behaviour among distance learners in the scholarship of learning and research work. The paper also amplifies the important role library plays in minimizing students’ sense of isolation in university learning experience.
Organizational effects and labor behavior of domestic violence
Authors: Castro-Gonzáles Segundo J. , Arias Díaz Orquídea , Irizarry Quintero Anamari 
Published: December 2016

Purpose
Using a proposed model, the purpose of this paper is to categorize small and medium enterprises’ (SMEs) owners/managers’ perceptions of customer service, and of the manifestations and observable behaviors in relation to the most frequent repercussions of domestic violence in the workplace.

Design/methodology/approach
A sample of 73 SME owners/managers was selected; a survey with 44 variables was used for data collection; and factorial and canonical analysis techniques were used to analyze the data.

Findings
The following were found: among the most frequent manifestations of domestic violence in SME customer services, there are four categories that explain 69.46 percent of the model of variance: threats and continuous absences, frequent mistakes and bad relationships, physical damage/lack of concentration and negative manifestation with low self-esteem; among observable behavior at the workplace, there are four categories that explain 65.35 percent of the model of variance: lack of concentration/tearfulness, fear/insecurity, limited attention to customers and continuous threats; finally there is a 96.70 percent probability of the SMEs’ productivity being affected (loss of customers and low production), through three manifestations of domestic violence detected in the employees: tiredness, sleepiness and tearfulness.

Research limitations/implications
This research is circumscribed to only one geographical zone in Puerto Rico.

Practical implications
Even only three manifestations of domestic violence in workers will significantly decrease productivity. When SME managers detect these, they can take action to mitigate the problem for the benefit of the workers.

Originality/value
This study is a pioneer proposal in its analytical quantitative approach on domestic violence in Puerto Rico and its repercussions on the productivity of the service sector, using canonical analysis.
Organizational structure and innovation: analysis from the strategic co-alignment
Authors: Marín-Idárraga Diego Armando , Cuartas Juan Carlos 
Published: December 2016

PurposeThe purpose of this paper is to determine the effect of the co-alignment of structural variables on innovation, wherein Bogotá-based SMEs, belonging to the industrial, commercial and services sectors, were used as samples.Design/methodology/approachThe study was conducted using a causal inference methodology and through the application of a structural equation model.FindingsThe results indicate that organizational structure has an influence on innovation, although this is true only for some variables and only when the analysis is performed separately. When performed under strategic co-alignment conditions, however, its influence becomes greater.Originality/valueOrganizational structure and innovation are determining factors of organizational performance. Although the strategic co-alignment theory has provided points of reference to understanding the phenomenon of organizational performance, there are still many gaps left to be filled. Additionally, it also requires empirical validation especially in relation to Colombian SMEs – and this is where this work makes a contribution.
Once learned, never forgotten: effective leadership development with social construction as a threshold concept
Authors: Billsberry Jon 
Published: December 2016

Purpose
This paper aims to discuss leadership development with social construction as the underpinning theory.

Design/methodology/approach
This is an autoethnographic study of the author’s insights from teaching leadership from a social construction perspective.

Findings
The social construction approach to leadership looks and behaves as a threshold concept in leadership. It takes students’ learning to a higher level, opens up a new curriculum, and changes their understanding of leadership for good.

Originality/value
The discovery that the social construction approach to leadership looks and behaves as a threshold concept suggests that there is something fundamentally important with this perspective. It positions the social construction approach as an overarching theory that helps students re-evaluate, appreciate, and find value in traditional leadership theories.