bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effectiveness of the use of disinfectants and specialized agents for the degradation of nucleic acid amplification products on laboratory surfaces
Authors:   Federal Budgetary Institution of Science Central Research Institute of Epidemiology of Rospotrebnadzor, Zamotaeva Tatyana L. , Trofimova Svetlana S. ,   Federal Budgetary Institution of Science Central Research Institute of Epidemiology of Rospotrebnadzor, Abrosimova Olga A. ,   Federal Budgetary Institution of Science Central Research Institute of Epidemiology of Rospotrebnadzor, Cherkashin Evgeniy A. ,   Federal Budgetary Institution of Science Central Research Institute of Epidemiology of Rospotrebnadzor, Akimkin Vasily G. ,   Federal Budgetary Institution of Science Central Research Institute of Epidemiology of Rospotrebnadzor
Published: September 2024

The rapid development of the PCR method opens up new opportunities for science and diagnostics. At the same time, the steady increase in the number of studies conducted increases the risk of contamination and, as a consequence, false-positive results. Today there are many ways to solve this problem, but none of them is universal. This paper presents the results of a comparison of the use of disinfectants and special decontamination agents to eliminate contamination of laboratory surfaces with nucleic acid amplification products – PCR amplicons. Study of decontamination activity against short DNA fragments of 117 bp were carried out under conditions that most closely simulate contamination of laboratory surfaces with PCR products. The effectiveness of decontamination was assessed by the residual number of amplicons in swabs from working surfaces. The high efficiency of destruction of amplicons when treating plastic, glass and metal surfaces with special decontamination agents based on metal ions and disinfectants based on oxygen-containing compounds has been shown. At the same time, it has been shown that agents containing a peroxide radical are capable of destroying amplicons mainly in combination with UV irradiation. Based on the results obtained, the feasibility of using metal ion-based decontamination agents for rapid and effective surface treatment in laboratories using nucleic acid amplification methods was demonstrated. Keywords: disinfectants, PCR, amplicons, decontamination.
Manajemen Stakeholder Kelompok Sadar Wisata dalam Pengembangan Wisata Budaya di Desa Rancakalong Sumedang
Authors: Syedira Sedranda , Widiowati Didiet 
Published: September 2024

Pengabdian masyarakat ini dilatarbelakangi oleh kerjasama antara Pemerintah Kabupaten Sumedang dengan Politeknik Kesejahteraan Sosial Bandung melalui pelaksanaan praktikum berbasis komunitas yang menyasar pada Indikator Kinerja Utama (IKU) yang tercantum dalam Rencana Kerja Pembangunan Daerah Kabupaten Sumedang menyangkut empat isu, salah satunya adalah peningkatan daya saing desa berupa one village one product. Berlandaskan pada potensi dan aset komunitas yang ada di wilayah Desa Rancakalong, maka pengabdian masyarakat diarahkan pada upaya pengembangan wisata berbasiske budayaan. Metode yang dilakukan dalam praktik pengabdian masyarakat adalah metode partisipatif, meliputi Focus Grup Discussion (FGD), wawancara, observasi, dan studi dokumentasi. Melalui manajemen stakeholder kelompok sadar wisata, praktik pengabdian diarahkan untuk melihat sejauh mana keterlibatan para tokoh yang selanjutnya munculah komitmen para tokoh dalam upaya pengembangan wisata budaya. Adapun hasil dari kegiatan pengabdian masyarakat ini berupa pemetaan kuadran aktor yang menunjukan empat macam posisi stakeholders berdasarkan kepentingan dan pengaruhnya yang outputnya berupa restruksturisasi kelompok sadar wisata dan penyusunan rencana kerja kelompok.

Kata Kunci : manajemen stakeholder, kelompok sadar wisata, desa wisata, aset komunitas.
Pemberdayaan Masyarakat Melalui Diversifikasi Program Bank Sampah di Desa Rancakalong Sumedang
Authors: Indriani Ellya Dwi , Widiowati Didiet 
Published: September 2024

Sampah merupakan salah satu masalah yang masih menjadi urgensi untuk segera diatasi, seperti halnya di Desa Rancakalong. Pengolahan sampah yang masih belum dilaksanakan karena tidak adanya TPA dan TPS di desa tersebut. Sebagian besar masyarakat hanya membakar sampah-sampah yang mereka hasilkan setiap harinya dan tidak jarang yang hanya membuang sembarangan di sepanjang Desa Rancakalong. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan penyadaran masyarakat tentang pentingnya pengolahan sampah melalui program Pemberdayaan Masyarakat Melalui Diversifikasi Bank Sampah. Kegiatan ini menggunakan metode partisipatif dengan melibatkan masyarakat secara utuh, serta penggalian data melalui observasi partisipatif, wawancara, studi dokumentasi dan FGD. Hasil dari kegiatan ini adalah terbentuknya TKM Bank Sampah, adanya penambahan pengetahuan dan keterampilan masyarakat, serta dampak kegiatan pada triple bottom line concept.
Perancangan dan Pendampingan Pembangunan Mushola Tahan Gempa di Desa Mekarlaksana, Kabupaten Cianjur
Authors: Syahriyah Dewi Rachmaniatus , Novis Putri Tika , Septian Ridho , Dira Nur Azizah  
Published: September 2024

Kegiatan Pengabdian kepada Masyarakat dilakukan berupa perancangan dan pendampingan pembangunan musholla tahan gempa di Desa Mekarlaksana, Kabupaten Cianjur yaitu Musholla Al Barokah. Abdimas berperan sebagai tenaga ahli yang melaksanakan perancangan hingga membantu koordinasi antara pelaksana konstruksi pembangunan. Tujuan dari pendampingan ini adalah untuk mewujudkan pembangunan Musholla Al Barokah yang sesuai dengan Rencana Teknis Pelaksanaan Pekerjaan baik dalam hal desain, maupun durasi waktu pelaksanaan konstruksi. Dalam rangka efisiensi pendanaan dan waktu, pada kegiatan ini, perancangan dan pendampingan pembangunan dilakukan dengan metode hybrid dengan memanfaatkan media Whatsapp. Penggunaan teknologi ini secara efektif membantu koordinasi antara abdimas, yayasan, maupun pelaksana lapangan sehingga permasalahan-permasalahan serta solusi cepat yang dibutuhkan dapat terakomodasi dengan baik. Luaran berupa terwujudnya perancangan dan pembangunan Mushola Tahan gempa di di Desa Mekarlaksana, Kabupaten Cianjur oleh Arsitek dan Tenaga Ahli Konstruksi yang sesuai, baik dalam segi koordinasi pelaksanaan, pengawasan pelaksanaan, penyelesaian permasalahan, serta ketepatan waktu
A new perspective on STEM education: The possible contributions of architectural education
Authors: Özkan Cem , Çepni Salih , Maratkyzy Nazerke , Vural Arslan Tülin , Durak Selen 
Published: September 2024

Considering the structural similarities between STEM education and architectural education, it is thought that architectural education, which has a deep-rooted history, may be useful for improving STEM education. This research was planned to gain useful inferences for STEM education by trying to get to know architectural education. In this study, ethnographic field research method was used. During the four-week observations, students made presentations with projects, models and plan drawings. In this process, teachers' criticisms and students' defenses were analyzed through the data table. By discussing the identified elements of architectural education, at least six innovations and/or meaningful results were revealed in the context of STEM education. These are: i-Students should be given the opportunity to solve open-ended problems on their own and should be encouraged to learn through trial and error in this process. ii-Students should be highly motivated when dealing with open-ended problems, for example, STEM project courses should be turned into graduation qualifications. iii-The importance of real-life context in STEM education should be emphasized and problems should be a part of life; In this context, sustainability and economic value dimensions should be highlighted. iv- At secondary and primary school levels, children should be encouraged to learn by experiencing and manipulating materials in the context of problems. v- Courses that will improve technical drawing skills should be added to STEM education programs. vi- In STEM disciplines, teachers should ensure theory/practice balance at the undergraduate level, and evaluations in applied projects should be made by a jury system.
Funcionalidad Familiar en Pacientes Adultos Integrados al Programa de Diálisis Peritoneal, de un Hospital de Referencia en el Municipio de Salina Cruz, Oaxaca
Authors: Velásquez García Karen , Rincon Peregrino Nallely 
Published: September 2024

La diálisis peritoneal (DP) es una de las principales modalidades de tratamiento para la enfermedad renal crónica (ERC) (Xie et al., 2018); sin embargo, se asocia con menor calidad de vida relacionada con salud (CVRS) y con alta mortalidad por sus complicaciones (Foreman et al., 2018). La funcionalidad familiar (FF) afecta la CVRS y puede orientar una mejor atención médica integral (Ku et al., 2019). El objetivo fue determinar la FF de pacientes con ERC en DP, mediante un estudio observacional y transversal que incluyó 115 pacientes de 40-55 años. El nivel de FF se estimó mediante APGAR familiar y el nivel de CVRS mediante escala KDQOL-36. El 59% fueron hombres y 41% mujeres; el 42% mostraron un nivel de FF funcional, el 34% mostraron disfunción severa, y en el 24% fue disfuncional. Pacientes con mejor nivel de FF reportaron más años en DP (p<0.01). El 40.8% contaban con CVRS buena, se observó menor FF en casos de CVRS deficiente (p<0.01). Los resultados sugieren que mayores niveles de FF se vinculan con mejor CVRS, con mejor control de la enfermedad y mayor sobrevida en uso de DP, por lo que es fundamental para el médico familiar determinar y fortalecer la FF en estos pacientes.
Consommateurs d’énergies, un concept-frontière pour l’histoire de l’énergie
Authors: Bouvier Yves 
Published: September 2024

L’approche historique des consommateurs d’énergies se définit par une multitude d’interrogations des concepts employés. Dans une anthropologie du quotidien, la consommation énergétique tend à disparaître derrière l’usage des objets, devenant une infra-consommation. La catégorie d’énergie même suppose une intégration des différentes sources d’énergie ce que les comportements des consommateurs ne permettent pas d’identifier, nous conduisant à les considérer davantage comme des « consommateurs d’énergies ». À partir de ce dialogue entre démarches empirique et déductive, les « consommateurs d’énergies » deviennent un concept pouvant nourrir les approches historiques actuelles (genre, histoire globale, transition…).
Effect of intraspecies interactions on Pseudomonas aeruginosa biofilm formation and gene expression
Authors: Calomino Mariana Alcântara , Silva Michelle Pereira , Pauer Heidi , Oliveira Beatriz Guitton Renaud Baptista de , Martins Francislene Juliana , Valente Gabriel Luis Cavalcanti , Paula Geraldo Renato de , Teixeira Felipe Lopes 
Published: September 2024

Pseudomonas aeruginosa is a common pathogen of acute and chronic infections that can increase risk to burned patients and those with chronic wounds, especially when forming biofilms. These complex ecosystems are formed by several bacteria that communicate, cooperate, and compete with each other. To enhance its survival capacity in such a competitive environment, P. aeruginosa produces secondary metabolites with a range of biological activities, among other factors. Here, we evaluated how such bioactive molecules mediate intraspecies interactions to modulate P. aeruginosa biofilm formation. An overall decrease in biofilm formation on the surface of stainless steel coupons was observed for both dual-strain and single-strain biofilms formed in the presence of secondary metabolites extracted from other P. aeruginosa strains. Modulation of biofilm formation by these metabolites during P. aeruginosa intraspecies interactions suggests a role for these molecules in strain competition. The most likely mechanism by which it occurs might be modulation of iron uptake. Among the genes analyzed, those related to import (tonB1) and biosynthesis (pvdS) of the siderophore pyoverdine were the most downregulated during exposure to the metabolite extracts. Exploring P. aeruginosa intraspecies interactions during biofilm formation might be an interesting approach to understand its lifestyle better and, thus, the mechanisms involved in the species’ biofilm formation.
Propofol Use in Emergency Surgery in a Patient Having Alcohol Withdrawal
Authors: Grover Sandeep , Naskar Chandrima , Jain Kajal , Aggarwal Sameer 
Published: September 2024

Abstract
Alcohol use disorder is a common mental health issue with life-threatening consequences like sustaining polytrauma while driving under the influence of alcohol. Such patients frequently require surgical interventions. Because of the alcohol dependence, they have a high risk of developing alcohol withdrawal (including delirium tremens) immediately before or sometimes after the surgery. No clear guidelines exist regarding the choice of anesthetic agent in this special population, even though the diagnosis of alcohol withdrawal and/or delirium can affect the outcome of the surgical intervention in multiple ways. At times, the anesthetic agent used during the surgical procedure can delay the onset of alcohol withdrawal delirium in these patients, in the postoperative period, thus complicating its identification and management. In this report, we present two scenarios in which the presence of alcohol withdrawal symptoms in the perioperative period was markedly affected by the use of infusion of propofol during the surgery. We also discuss how the mindful use of propofol infusion for anesthesia and analgesia during surgery can be a useful method, in addition to the usual benzodiazepine-based management, in this specific population to reduce the consequences of alcohol withdrawal in the postoperative period.
Masking ability of computer-aided design and manufacturing bleach shade ceramics with different thicknesses on titanium abutments
Authors: Bidaki Amin , Ahmadizenouz Ghazaleh , Esmaeili Behnaz 
Published: September 2024

ABSTRACT

Background:
Titanium abutments are the gold standard of implant treatments. However, they may not create sufficient color matching to the natural teeth due to the gray color showing through under the ceramic crown. The present study aimed to determine the masking ability of computer-aided design and manufacturing () bleach shade ceramics in different thicknesses on titanium abutments.

Materials and Methods:
In this laboratory study, a total of 90 specimens of bleach shade ceramics Celtra Duo (CD), Vita Suprinity (VS), and zirconia Luxen were prepared in thicknesses of 1, 1.5, and 2 mm (n = 10). Background specimens of G-aenial composite in A3 color and titanium were used. The ceramic specimens were placed on titanium and composite backgrounds and L*a*b* color parameters and color difference (ΔE) were measured with the VITA Easyshade spectrophotometer. Data were analyzed using three-way and one-way analysis of variance tests. Pairwise comparisons of groups were also performed with Tukey’s test. P <0.05 was considered statistically significant and ΔE <2.7 was considered clinically acceptable criteria.

Results:
The effects of thickness, ceramic type, and their interaction effects on the ΔE were statistically significant (all three: P <0.001). The difference in ΔE values of bleach shade ceramics on titanium and composite backgrounds was estimated to be statistically significant in the thicknesses of 1 mm (P = 0.01), 1.5 mm (P < 0.001), and 2 mm (P = 0.001). Zirconia had a better performance for masking ability in thicknesses of 1.5 mm (P < 0.001) and 1 mm (P = 0.01), while VS ceramic showed the best masking ability in thicknesses of 2 mm (P = 0.001). The masking ability of ceramics was improved by increasing the thickness.

Conclusion:
Except for VS ceramic in thickness of 1 mm, the rest of the bleach shade ceramics in all three thicknesses of 1, 1.5, and 2 mm have adequate ability to mask the titanium background and their use in line with the masking ability of titanium background has brought acceptable esthetic results.
Molecular Markers in Epithelial Dysplasia as Predictors of Malignant Transformation: A Review
Authors: Akula Sandhya Rani , Dorankula Shyam Prasad Reddy , Raj Yukti , Bhavani Sangala Nagendra 
Published: September 2024

Abstract
In the oral cavity, oncogenesis is widely believed to result from cumulative genetic alterations that cause a transformation of the mucosa from normal to dysplastic to invasive carcinoma. However, all epithelial dysplasias do not develop into cancer and some have shown to regress with time. Many potential markers have been used to diagnose the epithelial dysplasia and to predict their transformation into carcinoma such as p53 targets and family members: p53, MDM2, p16, p63, Akt. Therefore, identification of molecular and cellular markers specific to the oral lesions with potentially malignant transformation could lead to early detection, accurate diagnosis, prevention of the development of oral squamous cell carcinoma (OSCC). The present review highlights on a series of molecules markers that are reported to be significantly associated with progression of oral epithelial dysplasia to OSCC and are considered as potential biomarkers in predicting malignant transformation of epithelial dysplasias into OSCC.
Exogenous Janus Kinase 617 Codon Influences Small Noncoding RNAs and Gene Expression in Ba/F3 Cells
Authors: Chen Yi-Yang , Wang Ying-Hsuan , Chen Chih-Cheng , Huang Cih-En , Hsu Chia-Chen , Hsiao Shu-Huei , Leu Yu-Wei 
Published: September 2024

Abstract
Myeloproliferative neoplasms (MPNs) are blood cancers caused by mutations that originate from hematopoietic stem cells. More than 50%–90% of MPN patients had a dominant negative valine (V) to phenylalanine (F) mutation at the Janus kinase 617 codon (JAK2V617F) within the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway; however, this mutation was also found in a high percentage of the general population, its penetrance varied, and its onset was shown to be polygenic. Consequently, it is still unknown what molecular mechanism underlies the MPN transformation produced by JAK2V617F. Patients with MPN have been shown to have dysregulation of noncoding RNAs, such as microRNA (miRNA) and PIWI-interacting RNA (piRNA), although there is not any concrete proof that JAK2V617F alone is responsible for the aberrant regulation of miRNA and piRNA. Human wild type versus V617F-mutated JAK2 are expressed in mouse Ba/F3 cells, and the expressed small and total RNAs were subjected to next generation sequencing analysis to determine the direct induction. Differentially expressed miRNAs, gene expression, and transcript and gene variations were found between exogenously expressed JAK2 and JAK2V617F in Ba/F3 cells. The differently expressed variations contained enriched transposable elements and piRNAs, indicating a rearranged epigenome. The results of the pathway analysis show that the transformation that self-validated the chosen sequencing target genes is impacted by the JAK-STAT pathway. The induction route is functionally conserved, according to exogenously produced miRNA and gene expression. These results may clarify how the JAK2V617F induces transformation.
Incidence and Outcome of Newly Diagnosed Atrial Fibrillation in Medical ICU Patients: An Observational-Descriptive Study
Authors: Prakash Jyoti , Anil B. 
Published: September 2024

Abstract

Introduction:
Cardiac arrhythmias frequently occur in critically ill patients in the intensive care unit (ICU), significantly impacting their health outcomes. Among these arrhythmias, supraventricular cardiac arrhythmic tachycardia, particularly atrial fibrillation (AF), is increasing in incidence and spans various medical and surgical contexts. In medical patients, the incidence ranges from 1%–46%. In patients with sepsis, the incidence of acute AF varies with the severity of sepsis, with an incidence of 8–10% in sepsis, 6–22% in severe sepsis, and 23–44% in septic shock. The development of cardiac arrhythmias in critically ill patients poses a substantial risk to both morbidity and mortality.

Aim:
To study the incidence and outcome of new-onset AF (NOAF) in medical ICU patients.

Materials and Methods:
NOAF was identified in patients with documented sinus rhythm ECG upon admission to the medical ICU who later developed AF.

Results:
Four hundred and twenty patients underwent 24-h continuous ECG monitoring, with 68 developing new-onset arrhythmias. Among them, 21 developed AF (5%). AF predominated in patients with preexisting cardiac illness (76.1%), with pneumonia being the primary non-cardiac cause. Notably, among 21 patients with AF, 12 individuals (57.1%) experienced mortality, while 9 patients (42.8%) were discharged from medical care. Notably, among the discharged patients, 5 individuals (45.4%) continued to exhibit persistent AF.

Conclusion:
It has been observed that there is an increased incidence of AF among critically ill patients, correlating with heightened rates of mortality and morbidity. Early detection and treatment hold promise in altering disease trajectories. Hence, the implementation of 24-h ECG monitoring is advocated for critically ill patients. Furthermore, a significant fraction of acute illness survivors experience new-onset AF after being discharged, which raises their long-term risks of heart failure, ischemic stroke, and death. However, the lack of expert consensus documents or evidence-based guidelines for managing NOAF highlights the need for carefully planned multicenter, prospective randomized trials to address these clinical uncertainties.
High-Frequency Deep Sclerotomy in the Treatment of Primary Open-Angle Glaucoma in Pseudophakics: Our Experience at ECWA Eye Hospital, Kano, Nigeria
Authors: Atima Mayor , Idakwo Ugbede , Orugun Ayodele , Komolafe Oyeronke , Ajolomufiah Michael , Jah Pam Douglas 
Published: September 2024

Abstract

Background:
High-frequency deep sclerotomy (HFDS) is one of the treatment options for glaucoma and can be combined with cataract surgery when both conditions coexist. The procedure is also a good option to treat pseudophakics—found to have glaucoma after cataract surgery—that are not responding to treatment.
The aim of this study was to assess the efficacy and safety of HFDS in treatment of primary open-angle glaucoma (POAG) in pseudophakics.

Subjects and Methods:
This was a retrospective study of 34 patients who had HFDS in 2021. The intraocular pressures (IOPs) were obtained from the records of only 20 patients who completed 12 months of follow-up.
The MS Excel was used for the data compilation and description, and the Student t test was used for analysis.

Result:
The mean age of the patients was 65.9 ± 16.8 years consisting of 15 male patients and 5 female patients. The average preoperative IOP was 21.1 ± 6.9 and 14.25 ± 2.79 mm Hg postoperatively with P = 0.000563.

Conclusion:
HFDS being a minimally invasive technique is a viable option for lowering IOP in patients with POAG who had cataract surgery with posterior chamber intraocular lens. We recommend HFDS as a safe procedure that allows other conventional interventions for glaucoma treatment if further IOPlowering effects are needed in the future.