bluuebunny/crossref_metadata_2025_split huggingface dataset:
The MYC mRNA 3′‐UTR couples RNA polymerase II function to glutamine and ribonucleotide levels
Authors: Dejure Francesca R , Royla Nadine , Herold Steffi , Kalb Jacqueline , Walz Susanne , Ade Carsten P , Mastrobuoni Guido , Vanselow Jens T , Schlosser Andreas , Wolf Elmar , Kempa Stefan , Eilers Martin 
Published: April 2017

AbstractDeregulated expression of MYC enhances glutamine utilization and renders cell survival dependent on glutamine, inducing “glutamine addiction”. Surprisingly, colon cancer cells that express high levels of MYC due to WNT pathway mutations are not glutamine‐addicted but undergo a reversible cell cycle arrest upon glutamine deprivation. We show here that glutamine deprivation suppresses translation of endogenous MYC via the 3′‐UTR of the MYC mRNA, enabling escape from apoptosis. This regulation is mediated by glutamine‐dependent changes in adenosine‐nucleotide levels. Glutamine deprivation causes a global reduction in promoter association of RNA polymerase II (RNAPII) and slows transcriptional elongation. While activation of MYC restores binding of MYC and RNAPII function on most promoters, restoration of elongation is imperfect and activation of MYC in the absence of glutamine causes stalling of RNAPII on multiple genes, correlating with R‐loop formation. Stalling of RNAPII and R‐loop formation can cause DNA damage, arguing that the MYC 3′‐UTR is critical for maintaining genome stability when ribonucleotide levels are low.
Validation of New Quality Measures for Transitions Between Sites of Care
Authors: Parast Layla , Burkhart Q. , Desai Arti D. , Simon Tamara D. , Allshouse Carolyn , Britto Maria T. , Leyenaar JoAnna K. , Gidengil Courtney A. , Toomey Sara L. , Elliott Marc N. , Schneider Eric C. , Mangione-Smith Rita 
Published: April 2017

BACKGROUND AND OBJECTIVE:
Assessing and improving the quality of transitions to home from the emergency department (ED) or hospital is critical for patient safety. Our objective was to validate 8 newly developed caregiver-reported measures of transition quality.

METHODS:
This prospective observational study included 1086 caregiver survey respondents whose children had an ED visit (n = 523) or hospitalization (n = 563) at Seattle Children’s Hospital in 2014. Caregivers were contacted to complete 2 surveys. The first survey included the newly developed transition quality measures and multiple validation measures including modified versions of Child Hospital Consumer Assessments of Healthcare Providers and Systems (HCAHPS) composites, assessing communication and discharge planning. The second survey (administered 30 days later) included questions about follow-up appointments and ED return visits and readmissions. Using multivariate regression, we examined associations between the newly developed transition quality measures and each validation measure.

RESULTS:
All transition quality measures were significantly associated with ≥1 validation measures. The hospital-to-home transition measure assessing whether discharge instructions were easy to understand, were useful, and contained necessary follow-up information had the largest association with the Child HCAHPS nurse–parent and doctor–parent communication composites (β = 55.6; 95% confidence interval, 43 to 68.3; and β = 48.3; 95% confidence interval, 36.3 to 60.3, respectively, scaled to reflect change associated with a 0 to 100 change in the transition measure score).

CONCLUSIONS:
Newly developed quality measures for pediatric ED- and hospital-to-home transitions were significantly and positively associated with previously validated measures of caregiver experience. These new measures may be useful for assessing and improving on the quality of ED- and hospital-to-home transitions.
Language, gender and sexual identity: Poststructuralist perspectives Heiko Motschenbacher (2010) and An interdisciplinary bibliography on language, gender and sexuality (2000–2011) Heiko Motschenbacher (2012)
Authors: Acuña Ferreira A. Virginia 
Published: April 2017

Language, gender and sexual identity: Poststructuralist perspectives Heiko Motschenbacher (2010) Amsterdam and Philadelphia: John Benjamins. Pp. xii + 209 ISBN: 978 90 272 1868 1 (Hb; alk. Paper) ISBN 978 90 272 8750 2 (Eb) An interdisciplinary bibliography on language, gender and sexuality (2000–2011) Heiko Motschenbacher (2012)Amsterdam and Philadelphia: John Benjamins. Pp. vii + 294 ISBN: 978 90 272 1200 9 (Hb; alk. Paper) ISBN 978 90 272 7315 4 (Eb)
Gestão Participativa e a interface com trabalhadores do Sistema Único de Saúde: uma revisão integrativa
Authors: Gabriel Lopes Thais Rosental , Carvalho J. B. L. , Ramos N. O. F. , Câmara A. G. , Teixeira G. A. 
Published: April 2017

Trata-se de uma revisão integrativa de literatura com objetivo de integrar o conhecimento produzido acerca da Gestão Participativa e a interface dos trabalhadores do SUS. Para seleção dos artigos foram consultadas três bases de dados – Scielo, LiLACS e MEDLINE - sendo incluídos doze artigos. Os resultados mostram estudos qualitativos, todos publicados entre 2008 a 2012. Identificou-se nos artigos que a Gestão Participativa é vivenciada por trabalhadores de saúde, muito embora haja dificuldades para a mesma. Os resultados evidenciaram um maior número de estudos nas regiões sul e sudeste brasileiro. Conclui-se que a Gestão Participativa é importante para o desenvolvimento do SUS e que é possível superar desafios e fazer com que o trabalhador de saúde participe mais do processo de decisões.
VIOLÊNCIA SIMBÓLICA NA EPT – UM ESTUDO DE CASO NO CURSO TÉCNICO DE ENFERMAGEM DO INSTITUTO HUMBOLDT DE VITÓRIA/ES.
Authors: Bravo Rafaela Gomes 
Published: April 2017

Trata-se de um estudo de caso, que contou com a participação de três turmas do curso técnico de enfermagem, do Instituto Humboldt, da cidade de Vitória/ES. O trabalho se baseia no entendimento de Pierre Bourdieu, sociólogo francês, que por meio de sua teoria do habitus, afirma que a dominação sócio-econômica é majoritária e primeiramente reproduzida, pela dominação cultural. Em seus estudos voltados para a área educacional, constatou que é através da violência simbólica reproduzida no ambiente escolar, que a cultura dominante se naturaliza e se impõe como universal e superior. A partir deste entendimento, o presente trabalho teve como objetivo levantar os casos da violência simbólica no contexto pedagógico do curso técnico em enfermagem, verificar os problemas ocasionados por tal violência e identificar se os professores têm implementado ações que visem diminuir esses problemas. A pesquisa caracteriza-se como exploratória, qualitativa, se embasou em um estudo de caso, por meio de entrevistas semi-estruturadas. Após a análise fenomenológica dos dados, foi possível confirmar a hipótese levantada.
A CONCEPÇÃO DE FORMAÇÃO HUMANA NOS CURSOS DE LICENCIATURAS DO INSTITUTO FEDERAL DE EDUCAÇÃO CIÊNCIA E TECNOLOGIA DA PARAÍBA – IFPB
Authors: Lucena Francineide Fernandes 
Published: April 2017

O artigo visa analisar o conceito de formação humana presente nos projetos pedagógicos dos cursos de licenciatura em química e matemática ofertados pelo Instituto Federal de Educação, Ciência e Tecnologia da Paraíba- IFPB. Adotaremos o conceito de formação humana presente no pensamento gramsciano, o qual defende a indissociabilidade entre conhecimento histórico, práxis política e formação humana. Trata-se de estudo bibliográfico, onde foram consultados livros, artigos científicos, leis e os projetos pedagógicos dos referido cursos. Contata-se que os conceitos de formação docente e formação humana presentes nos projetos pedagógicos dos referidos cursos apresentam pontos de convergência com o pensamento gramsciano, ao buscarem uma formação omnilateral, privilegiando a interdisciplinaridade e a contextualização dos conhecimentos.
Kidney transplantation and bone disease: risk factors of development and diagnostics
Authors: Vetchinnikova O. N. 
Published: April 2017

Bone disease is a serious and common condition in patients after kidney transplantation. The review analyzed the causes of bone disorders in the early and late postoperative period that are associated with renal transplantation: fibroblast growth factor 23, parathyroid hormone, vitamin D, immunosuppressive therapy and imbalance of mineral metabolism. It shows the most common clinical variant of the post-transplant bone disease – secondary osteoporosis, risk factors of its development and complications. It presents the diagnostic algorithm for dynamic monitoring and evaluating the effectiveness of the treatment of bone disorders
Cystatin C in the diagnostics of acute kidney injury after heart transplantation
Authors: Strokov A. G. , Poz Ya. L. , Poptsov V. N. , Shevchenko A. O. , Shmerko N. P. 
Published: April 2017

Aim. To examine the assumption that significant concentrations of cystatin C in urine are the manifestation of the tubular necrosis and, respectively, the severity of kidney damage after heart transplantation (HTx).Materials and methods. In this study we evaluated 33 heart recipients (6 women and 27 men, aged from 24 to 68 years old) who had risk factors of acute kidney injury: serum creatinine level >113 μmol/l and/or mechanical circulatory support requirement (20 patients, in 14 cases before HTx). Cystatin C concentration in serum and in urine was measured by DyaSis particle-enhanced immunoturbidimetric assay test «Cystatin C FS».Results. Recipients were divided into two groups according to the levels of cystatinuria. In the group with the significant (more than 0.18 mg/l) urinary cystatin C concentrations the requirement of renal replacement therapy (RRT) was 2.5-fold higher, and the mean duration of RRT was more than 10-fold longer. In 2 patients with the significant cystatinuria acute kidney injury (AKI) has transformed into end-stage renal disease (ESRD).Conclusion. Due to data obtained we may suppose that significant concentrations of cystatin C in urine are the marker of the tubular necrosis with the prolonged RRT requirement. Further studies are needed to justify this relationship.
Perioperative period in cardiac transplantation from donors with brain death due to methanol poisoning
Authors: Poptsov V. N. , Spirina E. A. , Pchelnikov V. V. , Ukhrenkov S. G. , Masyutin S. A. , Voronkov V. Yu. , Aliev E. A. , Ustin S. Yu. 
Published: April 2017

The successful use of donor hearts from people died of methanol poisoning helps reducing the deficit of donor organs for patients requiring urgent cardiac transplantation [3]. We present our experience of successful cardiac transplantations from 2 donors who died due to methanol poisoning. Given the possibility of performing a cardiac transplant from this group of donors a protocol has been developed at the V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation which includes clinical, laboratory and instrumental criteria for the selection of heart donor and recipient. The possibility of delayed onset myocardial contractile dysfunction due to methanol poisoning means that a longer conditioningperiod is vital as well as compulsory clinical, laboratory and expert chocardiographic examinations of the potential donor heart.
Kidney injury and renal replacement therapy in heart transplant recipient
Authors: Poz Ya. L. , Strokov A. G. , Poptsov V. N. , Kopylova Yu. V. , Kryshin K. N. 
Published: April 2017

The development of kidney injury and the characteristics of renal replacement therapy were considered in patient with dilated cardiomyopathy, who consequently underwent two heart transplantations and two kidney transplantations. Since the number of the patients needed both kidney and heart transplantation increases constantly, the multifaceted thorough research in this specific patient population is extremely important.
Cellular neuroprotection as a modern treatment approach for optic neuropathy
Authors: Borzenok S. A. , Khubetsova M. Kh. , Saburina I. N. , Gavrilova N. A. , Tonaeva Kh. D. , Ostrovskiy D. S. , Lanevskaya N. I. , Kosheleva N. V. , Zurina I. M. 
Published: April 2017

Aim.To develop technology to create 3D-spheroid multipotent mesenchymal stem cells (MMSC) of limbal cadaveric human eyes, capable of safe and long-term secretion of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF).Materials and methods.MMSC were obtained by cultivation of limbal fragments, released from cadaveric donor human eye. Cultivation was carried out in DMEM/F12 medium, supplemented with L-glutamine, penicillin, streptomycin, amphotericin B, HEPES, insulin, dexamethasone and 10 vol.% FBS under standard conditions (5% СО2, 37 °C), medium change was performed every 3 days. To determine the phenotype of the received cell culture the method of immunophenotyping by marker proteins to MMSC (CD73, CD105, CD19, CD90, CD133) was used. Stimulation of neurotrophic factor secretion was performed via a twostep procedure. 3D-cell spheroids were created with the help of agarous plates for three groups of comparison, where group I was control group, spheroids of intact 2D-culture MMSK; group II – spheroids of previously induced 2D-culture MMSC; group III – spheroids of 2D-MMSC induced on the 1st day of cultivation. Cell cultures supernatants were selected in different periods for NGF and BDNF follow-up study by ELISA procedure.Results. Induction of 3D-spheroids of limbal MMSC promotes short-term increase of the level of BDNF and NGF, but further, the secretion of these factors significantly decreases. Induction leads to a change in the morphology of spheroids: loss of compactness and emergence of «fringed» (debris). Such changes indicate of frailty of received constructions. Spheroids from previously induced MMSC are capable of stable NTF secretion, but the level of secretion is much less as compared to the control group.Conclusion.3D-cell culture of intact 2Dculture of limbal MMSC can be considered as cellular medication for a safe and long-term neuroprotection in optic neuropathy treatment.
Assessment Methods of Quality of Life of Living Organ Donors
Authors: Gautier S. V. , Konstantinov V. K. 
Published: April 2017

The study of quality of life of living organ donors in transplantation by questionnaires has increasingly become the subject of clinical studies. However, there are no unified criteria and standard norms of quality of life. Each questionnaire has its own criteria and evaluation scale. This review presents the main general questionnaires that have been applied worldwide for the quality of life assessment of living kidney and liver donors. Special questionnaires used to refine the parameters of quality of life and their coordination with common questionnaires are considered. Optimal questionnaire to detect quality of life in modern research for living organ donors is identified.
A Host-Dependent Prognostic Model for Elderly Patients with Diffuse Large B-Cell Lymphoma
Authors: Miura Katsuhiro , Konishi Jun , Miyake Takaaki , Makita Masanori , Hojo Atsuko , Masaki Yasufumi , Uno Masatoshi , Ozaki Jun , Yoshida Chikamasa , Niiya Daigo , Kitazume Koichi , Maeda Yoshinobu , Takizawa Jun , Sakai Rika , Yano Tomofumi , Yamamoto Kazuhiko , Sunami Kazutaka , Hiramatsu Yasushi , Aoyama Kazutoshi , Tsujimura Hideki , Murakami Jun , Hatta Yoshihiro , Kanno Masatoshi 
Published: April 2017

Abstract

Background
Decision-making models for elderly patients with diffuse large B-cell lymphoma (DLBCL) treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) are in great demand.

Patients and Methods
The Society of Lymphoma Treatment in Japan (SoLT-J), in collaboration with the West-Japan Hematology and Oncology Group (West-JHOG), collected and retrospectively analyzed the clinical records of ≥65-year-old patients with DLBCL treated with R-CHOP from 19 sites across Japan to build an algorithm that can stratify adherence to R-CHOP.

Results
A total of 836 patients with a median age of 74 years (range, 65–96 years) were analyzed. In the SoLT-J cohort (n = 555), age &gt;75 years, serum albumin level &lt;3.7 g/dL, and Charlson Comorbidity Index score ≥3 were independent adverse risk factors and were defined as the Age, Comorbidities, and Albumin (ACA) index. Based on their ACA index score, patients were categorized into “excellent” (0 points), “good” (1 point), “moderate” (2 points), and “poor” (3 points) groups. This grouping effectively discriminated the 3-year overall survival rates, mean relative total doses (or relative dose intensity) of anthracycline and cyclophosphamide, unanticipated R-CHOP discontinuance rates, febrile neutropenia rates, and treatment-related death rates. Additionally, the ACA index showed comparable results for these clinical parameters when it was applied to the West-JHOG cohort (n = 281).

Conclusion
The ACA index has the ability to stratify the prognosis, tolerability to cytotoxic drugs, and adherence to treatment of elderly patients with DLBCL treated with R-CHOP.
Oncofertility: Fertile Ground for Conflict Between Patient Autonomy and Medical Values
Authors: Walsh Sarah K. , Ginsburg Elizabeth S. , Lehmann Lisa Soleymani , Partridge Ann H. 
Published: April 2017

Abstract
Oncofertility is a unique, multidisciplinary field that serves to bridge the gap between available fertility resources and the special reproductive needs of cancer patients. Oncofertility is a growing field due to the increasing number of survivors, development of new oncologic therapies, extension of duration of therapies, and development and refinement of reproductive therapies. While the technologies and demand for services expand, clinicians need to be appropriately prepared for dealing with various clinical scenarios that may require ethical deliberation. Three real cases are presented in which the patient wishes to pursue reproductive assistance, but her decision is met with hesitance or uncertainty by her care team. Discussion of these clinical scenarios highlights ethical implications of oncofertility practice and serves to highlight the need for the establishment of multidisciplinary care teams and guidelines to support both clinicians and patients.
Data Linkage to Improve Geriatric Oncology Research: A Feasibility Study
Authors: Lund Jennifer L. , Meyer Anne-Marie , Deal Allison M. , Choi Bong-Jin , Chang YunKyung , Williams Grant R. , Pergolotti Mackenzi , Guerard Emily J. , Muss Hyman B. , Sanoff Hanna K. 
Published: April 2017

Abstract
Older adults (aged 65 years and older) diagnosed with cancer account for most cancer-related morbidity and mortality in the United States but are often underrepresented on clinical trials. Recent attention from a variety of professional, research, regulatory, and patient advocacy groups has centered on data linkage and data sharing as a means to capture patient information and outcomes outside of clinical trials to accelerate progress in the fight against cancer. The development of a more robust observational research data infrastructure would help to address gaps in the evidence base regarding optimal approaches to treating cancer among the growing and complex population of older adults. To demonstrate the feasibility of building such a resource, we linked information from a sample of older adults with cancer in North Carolina using three distinct, but complementary, data sources: (a) the Carolina Senior Registry, (b) the North Carolina Central Cancer Registry, and (c) North Carolina fee-for-service Medicare claims data. A description of the linkage process, metrics, and characteristics of the final cohort is reported. This study highlights the potential for data linkage to improve the characterization of health status among older adults with cancer and the possibility to conduct passive follow-up for outcomes of interest over time. Extensions of these linkage efforts in partnership with other institutions will enhance our ability to generate evidence that can inform the management of older adults with cancer.
Managing the Trade-Public Health Linkage in Defence of Trade Liberalisation and National Sovereignty: An Appraisal of United States-Measures affecting the Production and Sale of Clove Cigarettes
Authors: Warikandwa Tapiwa Victor , Osode Patric C 
Published: April 2017

Under the legal framework of the World Trade Organisation (WTO), countries have great flexibility to unilaterally adopt environmental regulations that have effect within their territories only. However, the same discretion does not apply to measures that adversely affect imports or exports. An absence of clear guidelines on how to address some of the attendant issues poses challenges to the effectiveness of a trade-environment linkage. Not surprisingly, attempts to link the environment and trade have resulted in a number of jurisprudentially significant cases in which the WTO's Panel and Appellate Body have tried to address critical questions about the Organisation's capacity to address or manage legal or quasi-legal subjects falling outside the scope of its legal framework. In this regard the Panel and Appellate Body reports in the case of United States - Measures Affecting the Production and Sale of Clove Cigarettes (US-Clove Cigarettes) have re-ignited the debate on the Organisation's existential challenge of balancing the rights of the sovereign to freely regulate matters pertaining to health or the environment within its domestic domain with the need to maintain the sanctity of the multilateral trade order. This article demonstrates that in the US-Clove Cigarettes case the WTO Panel and Appellate Body, whilst managing to successfully defend the integrity of WTO Member States' treaty commitments and the overarching importance of trade liberalisation within the organisation's policy foundations even in the context of public health-related regulations, failed to provide any substantive affirmation of the development-related challenges facing developing countries that are part of the WTO family.
PEMANFAATAN GLISEROL DARI PRODUK SAMPING BIODIESEL UNTUK SINTESIS SENYAWA 2,3-DIBROMO PROPANOL
Authors: Megawati Dewi Sinta , Jumina Jumina , Pranowo Harno Dwi 
Published: April 2017

<p>Telah dilakukan sintesis senyawa 2,3-dibromo propanol menggunakan bahan awal gliserol hasil isolasi produk samping pembuatan biodiesel. Peningkatan yang cepat dalam produksi biodiesel diperkirakan akan mengakibatkan surplus gliserol. Penggunaan gliserol sebagai bahan awal bertujuan meningkatkan nilai ekonomis dari gliserol itu sendiri. Gliserol dengan rendemen 88,7 % telah diisolasi dari produk samping pembuatan biodiesel melalui reaksi transesterifikasi trigliserida dalam minyak sawit menggunakan katalis basa KOH. Selanjutnya, gliserol didehidrasi dengan asam format untuk mendapatkan senyawa alil alkohol melalui metode destilasi pada 195-260 <sup>0</sup>C. Sintesis senyawa 2,3-dibromo propanol dicapai melalui brominasi alil alkohol pada -2-0 <sup>0</sup>C menggunakan pelarut khloroform. Karakterisasi struktur dilakukan menggunakan spektrometer IR, <sup>1</sup>H-NMR, dan GC-MS. Pada penelitian ini, alil alkohol yang diperoleh melalui metode destilasi memiliki rendemen sebesar 44,78 % dan reaksi brominai alil alkohol menghasilkan senyawa 2,3-dibromo propanol dengan rendemen 80,3 % yield.</p><p> </p>Keywords : <em>senyawa</em> <em>2,3-dibromo propanol, gliserol, biodiesel</em>