bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Micropropagation of Eria Lasiopetala (Willd.) Ormerod- A Native Medicinal Orchid in Bangladesh
Authors: Ayesha Mst Naznin , Saha Setu Rani , Islam S M Shahinul 
Published: September 2024

The present study focuses on the asymbiotic germination of seeds through in vitro culture of Eria lasiopetala, a native medicinal orchid in Bangladesh. Out of the four basal media, MS was found to be the most effective medium, where 93.58±0.374% seed germinations were recorded. Different plant growth regulators (PGRs), e.g. BAP, Kin, NAA and were used for secondary protocorm proliferation, plantlet development, and multiplication of shoot buds through in vitro conditions. The maximum number of secondary protocorms (12.80±0.115) were developed that were derived from primary protocorms on MS medium fortified with 0.5 mg/l BAP and 1.0 mg/l NAA. Data revealed that 0.5 mg/l BAP + 0.25 mg/l NAA showed the best combination for height elongation of plantlets (3.12±0.012 cm) in MS medium. While for multiple shoot buds (MSBs) 1.0 mg/l BAP + 0.5 mg/l NAA showed better (6.8±0.127) performance than other combinations of PGRs. The single shoots were isolated from the multiple shoots and sub-cultured on ½MS medium supplemented with NAA (0.5 - 2.0 mg/l), IAA (0.5 - 2.0 mg/l) and IBA (0.5 - 2.0 mg/l) for root induction. The maximum number of roots (7.53±0.176) and its growth (4.61±0.01 cm) were found in ½MS medium supplemented with 1.0 mg/l IAA. The well rooted plantlets were hardened and successfully transferred after acclimatization in pots containing the mixture of coconut husk, charcoal and brick pieces in the ratio of 2:1:1 and eventually established under natural condition.
J. Bio-Sci. 32(1): 45-60, 2024
Penggunaan Multimedia Development Life Cycle dalam Rancang Bangun Media Pembelajaran Toleransi Beragama
Authors: Fatimah Dini Destiani Siti , Mubarok Muhammad Syauqi , Apriani Aulia 
Published: September 2024

Seiring perkembangan zaman, media interaktif menjadi salah satu alternatif metode pembelajaran. Pembelajaran menggunakan media interaktif memiliki nilai tambah pada proses pendidikan, salah satu bidang pembelajaran yang membutuhkan inovasi yaitu pembelajaran agama. Pemerintah Indonesia mengakui adanya keragaman agama, di mana toleransi beragama perlu disosialisasikan kepada pemeluknya terutama anak-anak. Materi toleransi beragama ditinjau dari berbagai agama dapat disosialisasikan melalui media pembelajaran berbasis teknologi informasi. Media tersebut digolongkan kepada media interaktif yang mendukung keefektifan pembelajaran karena memanfaatkan multimedia. Tujuan penelitian ini untuk merancang bangun media pembelajaran mengenai toleransi beragama dengan metode Multimedia Development Life Cycle. Metode ini memiliki enam tahapan yaitu tahap-tahap concept, design, material collecting, assembly, testing, dan distribution. Aplikasi ini telah diuji menggunakan uji fungsional serta uji beta yang dilakukan bersama guru. Hasil pengujian menunjukkan fungsi-fungsi telah berjalan sesuai dengan yang diharapkan. Hasil penelitian ini dapat berperan dalam memahamkan anak-anak mengenai toleransi beragama melalui materi toleransi beragama yang sesuai dengan agama masing-masing.
تأثير تعلم النحو "العمريطي" على كفاءة قراءة الكتب التراثية لطلبة الفصل الثاني في المعهد الإسلامي "منبع الفتوح" طوبان
Authors: Zaidah Faza Fauziataz 
Published: September 2024

ABSTRAK
Artikel ini mengkaji tentang pengaruh kegiatan mengaji nahwu pada kemampuan membaca kitab klasik murid kelas 2 di pondok pesantren putri manbail futuh Tuban. penelitian ini bertujuan untuk (1) Untuk mengetahui kegiatan mengaji kitab Imrithi santri kelas 2 di Pondok Pesantren Putri Manbail Futuh Tuban. (2) Untuk mengetahui kemampuan membaca kitab kuning di Pondok Pesantren Putri Manbail Futuh Tuban. (3) Untuk mengetahui pengaruh kegiatan mengaji kitab Imrithi santri kelas 2 pada kemampuan membaca kitab kuning di Pondok Pesantren Putri Manbail Futuh Tuban. Metode yang digunakan Kuantitatif dengan test, Angket, wawancara, dan dokumentasi. kemudian dianalisis dengan menggunakan uji produk moment , Adapun Hasil penelitian: (1) kegiatan mengaji nahwu di kelas 2 kelas 2 di pondok pesantren putri manbail futuh Tuban berjalan dengan baik. (2) kemampuan membaca kitab santri kelas 2 di pondok pesantren putri manbail futuh berjalan dengan baik. Namun, hasil tes menunjukkan bahwa nilai baca kitab masih tergolong rendah.(3) setelah dilakukan berbagai uji pada hasil angket dan tes yang telah dilakukan, ditemukan hasil bahwa tidak ada pengaruh dari kegiatan mengaji nahwu pada kemampuan membaca kitab kuning santri di pondok pesantren putri manbail futuh.
Kata kunci: Nahwu, Kemampuan Membaca
Textile omnidirectional antenna for wearable WiFi-7 applications using higher order modes
Authors: Noghanian Sima 
Published: September 2024

In WiFi 7 (IEEE 802.11be), Multiple Input Multiple Output (MIMO) technology plays a crucial role in enhancing throughput. This combination enables numerous applications for wearable WiFi devices. For instance, WiFi can be utilized for tracking and fall detection purposes. Additionally, wearable devices used in gaming, vital signal monitoring, and tracking can benefit from the implementation of wearable MIMO antennas. Despite the demand for wearable WiFi antennas, specifically in the new 6 GHz band, a significant gap exists in the investigation of antennas that are completely made of textile, are low profile, and provide vertical polarization and omnidirectional patterns. Addressing this gap, in this paper, a wearable planar antenna is introduced, which is specifically designed to offer an omnidirectional pattern and linear polarization. To ensure its practicality and ease of use, the antenna utilizes lightweight and wearable textile material. The design details and performance of the proposed antenna are presented. The antenna covers all three bands of WiFi, namely, 2.4 GHz, 5 GHz, and 6 GHz bands. The 10-dB return loss bandwidth is 2.4 GHz–2.5 GHz, and 4.6 GHz–7.2 GHz. The maximum Specific Absorption Rate (SAR) for an input power of 500 mW is calculated at 1.053 W/Kg for a 2 mm air gap between the antenna and tissue surface. The size of the antenna is given by a circular area of π × (50 mm)2, and a thickness of 6 mm.
Changes in the epidemiological patterns of respiratory syncytial virus and human metapneumovirus infection among pediatric patients and their correlation with severe cases: a long-term retrospective study
Authors: Kuang Lu , Xu Tiantian , Wang Changbing , Xie Jiahui , Zhang Yingying , Guo Min , Liang Zhuofu , Zhu Bing 
Published: September 2024

ObjectivesWe aim to investigate the prevalence of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) among pediatric patients with acute respiratory tract illness (ARTI) in southern China both pre- and post-COVID-19 pandemic, as well as identify associated risk factors for severe infections.MethodsThe study conducted a real-time PCR analysis on hospitalized children with ARTI from 2012 to 2023, specifically targeting RSV, hMPV, and other respiratory pathogens. Additionally, demographic data was collected during this analysis.ResultsThe prevalence of RSV occurs triennially, and likewise, the temporal pattern of hMPV outbreaks mirrors that of RSV. The peak infection rates of RSV and hMPV occurred during and following the implementation of COVID-19 epidemic prevention and control measures. The incidence of RSV infection exhibited bimodal peaks in 2022, while hMPV demonstrated seasonal peaks during the spring, fall, and winter periods post-COVID-19 pandemic. After the COVID-19 outbreak, there has been an upward trend in the proportion of female patients and patients aged one year and older presenting with ARTI, RSV infections, and hMPV infections. Infant (OR = 4.767, 95%CI: [3.888–5.846], p &lt; 0.0001), presence of co-infection (OR = 0.540, 95%CI: [0.404–0.722], p &lt; 0.0001), and existence of comorbidities (OR = 1.582, 95%CI: [1.285–1.949], p &lt; 0.0001) was the risk ratio for the severity of RSV infection. Children infected with hMPV under the age of 1 year (OR = 0.322, 95%CI: [0.180 – 0.575], p &lt; 0.0001), as well as those with comorbidities (OR = 8.809, 95%CI: [4.493 – 17.272], p &lt; 0.0001), have a higher risk of developing severe illness.ConclusionThe changing epidemiological patterns have the potential to lead to widespread severe outbreaks among children, particularly those with underlying medical conditions who may experience more severe symptoms. Conducting surveillance for pneumoviridae viruses in children is an imperative measure to establish a robust foundation for future epidemic prevention and treatment strategies.
Envirotyping helps in better understanding the root cause of success and limitations of rainfed production systems
Authors: Sawargaonkar Gajanan L. , Davala Moses Shyam , Rakesh S. , Kamdi Prasad J. , Khopade Rohan Y. , Nune Rajesh , Pasumarthi Rajesh , Choudhari Pushpajeet , Datta Aviraj , Akuraju Venkata Radha , Dixit Sreenath , Singh Ramesh , Jat Mangi Lal 
Published: September 2024

The current diagnostic agronomy study of the Bankura region of West Bengal, India, examined the variations in crop yields through a socio-ecological analysis of multiple production system components. Envirotyping for root cause analysis was employed to delve into the variables that affect the performance of rainfed production systems. Mother Earth, man, machine, management, and materials (5Ms concept) were the five indicators under which the variables were grouped. Findings demonstrated the fragility of the region’s soils due to its undulating terrain, unpredictable rainfall patterns, and frequent drought scenarios. The LULC’s NDVI showed that the agricultural area is about 60% and 43% of the total geographical area in the Hirbandh and Ranibandh blocks, respectively. Soils are acidic and diagnosed with deficiency of both macro and micronutrients (phosphorous, sulfur, and boron) having poor water holding capacity (35 to 55 mm for a 50 cm soil depth). The sand and soil organic carbon contents ranged between 43.04%–82.32% and 0.17%–1.01%, respectively with a low bacterial population. These factors are the root cause for low cropping intensity (106%) and low paddy productivity (3,021 kg/ha). Overall, the study contributes to designing and scaling-up of sustainable landscape management practices that could ensure higher cropping intensity and system productivity in similar agro-ecologies with limited evidence.
Causal association of inflammatory bowel disease with sarcoidosis and the mediating role of primary biliary cholangitis
Authors: Yi Jiazhi , Wu Shuyun , He Hongxia 
Published: September 2024

ObjectivesPrevious observational epidemiological studies have identified a potential association between inflammatory bowel disease (IBD) and sarcoidosis. Nonetheless, the precise biological mechanisms underlying this association remain unclear. Therefore, we adopted a Mendelian randomization (MR) approach to investigate the causal relationship between IBD with genetic susceptibility to sarcoidosis, as well as to explore the potential mediating role.MethodsThe genetic associations were obtained from publicly available genome-wide association studies (GWASs) of European ancestry. The IBD dataset has 31,665 cases and 33,977 controls, consisting of 13,768 individuals with ulcerative colitis (UC) and 17,897 individuals with Crohn’s disease (CD). The genetic associations of sarcoidosis with 4,854 cases and 446,523 controls. A bidirectional causality between IBD and sarcoidosis was implemented to be determined by a two-sample MR approach. The inverse variance weighted (IVW) method was utilized as the main statistical method, and a series of sensitivity analyses were performed to detect heterogeneity and horizontal pleiotropy. A two-step MR approach was used to investigate whether the mediating pathway from IBD to sarcoidosis was mediated by PBC.ResultsThe forward MR analysis indicated that genetic predisposition to IBD was significantly linked to an increased risk of sarcoidosis (OR = 1.088, 95% CI: 1.023–1.158, pIBD-sar = 7.498e-03). Similar causal associations were observed in CD (OR = 1.082, 95% CI: 1.028–1.138, pCD-sar = 2.397e-03) and UC (OR = 1.079, 95% CI: 1.006–1.158, pUC-sar = 0.034). Reverse MR analysis revealed that genetic susceptibility to sarcoidosis was correlated with an augmented risk of CD (OR = 1.306, 95% CI: 1.110–1.537, psar-CD = 1.290e-03) but not IBD or UC. The mediation analysis via two-step MR showed that the causal influence of IBD and CD on sarcoidosis effects was partly mediated by PBC, and the mediating effect was 0.018 (95% CI: 0.005–0.031, p = 7.596e-03) with a mediated proportion of 21.397% in IBD, and 0.014 (95% CI: 0.004–0.024, p = 7.800e-03) with a mediated proportion of 17.737% in CD.ConclusionsThe MR analysis provided evidence substantiating the causal effect of IBD (CD and UC) on an increased risk of sarcoidosis, with PBC playing a mediating role in IBD and CD. However, sarcoidosis only enhances the risk of developing CD, but not IBD or UC. These findings illuminate the etiology of sarcoidosis and contribute to the management of IBD patients.
Exploring the transcriptomic landscape of moyamoya disease and systemic lupus erythematosus: insights into crosstalk genes and immune relationships
Authors: Guo Qingbao , Fan Yan-Na , Xie Manli , Wang Qian-Nan , Li Jingjie , Liu Simeng , Wang Xiaopeng , Yu Dan , Zou Zhengxing , Gao Gan , Zhang Qian , Hao Fangbin , Feng Jie , Yang Rimiao , Wang Minjie , Fu Heguan , Bao Xiangyang , Duan Lian 
Published: September 2024

BackgroundSystemic Lupus Erythematosus (SLE) is acknowledged for its significant influence on systemic health. This study sought to explore potential crosstalk genes, pathways, and immune cells in the relationship between SLE and moyamoya disease (MMD).MethodsWe obtained data on SLE and MMD from the Gene Expression Omnibus (GEO) database. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were conducted to identify common genes. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on these shared genes. Hub genes were further selected through the least absolute shrinkage and selection operator (LASSO) regression, and a receiver operating characteristic (ROC) curve was generated based on the results of this selection. Finally, single-sample Gene Set Enrichment Analysis (ssGSEA) was utilized to assess the infiltration levels of 28 immune cells in the expression profile and their association with the identified hub genes.ResultsBy intersecting the important module genes from WGCNA with the DEGs, the study highlighted CAMP, CFD, MYO1F, CTSS, DEFA3, NLRP12, MAN2B1, NMI, QPCT, KCNJ2, JAML, MPZL3, NDC80, FRAT2, THEMIS2, CCL4, FCER1A, EVI2B, CD74, HLA-DRB5, TOR4A, GAPT, CXCR1, LAG3, CD68, NCKAP1L, TMEM33, and S100P as key crosstalk genes linking SLE and MMD. GO analysis indicated that these shared genes were predominantly enriched in immune system process and immune response. LASSO analysis identified MPZL3 as the optimal shared diagnostic biomarkers for both SLE and MMD. Additionally, the analysis of immune cell infiltration revealed the significant involvement of activation of T and monocytes cells in the pathogenesis of SLE and MMD.ConclusionThis study is pioneering in its use of bioinformatics tools to explore the close genetic relationship between MMD and SLE. The genes CAMP, CFD, MYO1F, CTSS, DEFA3, NLRP12, MAN2B1, NMI, QPCT, KCNJ2, JAML, MPZL3, NDC80, FRAT2, THEMIS2, CCL4, FCER1A, EVI2B, CD74, HLA-DRB5, TOR4A, GAPT, CXCR1, LAG3, CD68, NCKAP1L, TMEM33, and S100P have been identified as key crosstalk genes that connect MMD and SLE. Activation of T and monocytes cells-mediated immune responses are proposed to play a significant role in the association between MMD and SLE.
Cardiometabolic multimorbidity in Mexican adults: a cross-sectional analysis of a national survey
Authors: Agudelo-Botero Marcela , Dávila-Cervantes Claudio A. , Giraldo-Rodríguez Liliana 
Published: September 2024

BackgroundCardiometabolic multimorbidity is a rising phenomenon that has been barely explored in middle-income countries such as Mexico.ObjectiveThis study aimed to estimate the prevalence, associated factors, and patterns of cardiometabolic multimorbidity (2 and 3+ diseases) in Mexican adults (≥20 years old) by age group.MethodsA cross-sectional and secondary analysis of Mexico’s National Health and Nutrition Survey 2018–2019 was conducted. Information on eight diseases and other sociodemographic and health/lifestyle characteristics was obtained through self-reporting. Descriptive analyses were performed, and multinomial logistic regression models were calculated to identify the variables associated with cardiometabolic multimorbidity. Factor analysis and latent classes were estimated to determine disease patterns.ResultsThe prevalence of cardiometabolic multimorbidity for the total population study was 27.6% (13.7% for people with 2 diseases and 13.9% for people with 3+ diseases). By age group, the prevalence of 2+ diseases was 12.5% in the age group of 20–39 years, 35.2% in the age group of 40–59 years, and 44.5% in the age group of 60 years and older. The variables of depressive symptomatology and having functional limitations (1+) were statistically associated with cardiometabolic multimorbidity in almost all age groups. Patterns of cardiometabolic multimorbidity varied among adults in different age groups. Understanding the behavior of cardiometabolic multimorbidity at various stages of adulthood is a resource that could be used to design and implement intervention strategies. Such strategies should correspond to the population’s sociodemographic, health, and lifestyle characteristics and the specific disease patterns of each age group.
Health professionals’ views and experiences of breaking bad news in the Eastern Mediterranean Region: a scoping review
Authors: Yousuf Abdulla Ahmad A. A. , Stewart Derek Charles , Kane Tanya , Soltani Abderrezzaq , Al-Khal Abdullatif , Sethi Ahsan 
Published: September 2024

IntroductionBreaking bad news is a critical yet challenging aspect of healthcare that requires effective communication skills, empathy, and cultural sensitivity. Health professionals in the World Health Organization’s (WHO) Eastern Mediterranean Region face unique cultural and social factors distinct from other parts of the world. This scoping review aims to comprehensively explore the peer-reviewed literature on the health professionals’ experiences in delivering bad news within the WHO’s Eastern Mediterranean Region.MethodsThis scoping review was conducted according to the Joanna Brigg Institute’s scoping review methodology and reported utilizing the Preferred Reporting Items for Systematic Reviews extension for scoping review (PRISMA-ScR) guidelines. A search using a combination of keywords and MeSH terms related to “breaking bad news” and “health professionals” was performed in PubMed, Scopus, CINAHL, EBSCO, ERIC via Embase, and Dar Almandumah (Arabic) databases. Common themes were synthesized from studies conducted in the WHO’s Eastern Mediterranean Region.ResultsOut of 4,883 studies initially identified in the databases, 24 studies met the inclusion criteria, involving a total of 4,710 participants, including physicians, nurses, and residents. The studies were published between 2006 and 2022, predominantly from Iran (n = 12). The majority employed a cross-sectional design (n = 21) or mixed methods (n = 3), with a notable absence of qualitative studies. No studies used theoretical frameworks. More than half of the studies (n = 14) reported that participants had positive attitudes toward breaking bad news. This positivity was evident in their willingness to share bad news, perceived possession of adequate knowledge, positive attitudes, having received training, awareness of accepted approaches, and adherence to protocols. The lack of training and limited awareness of established protocols like SPIKES, ABCDE, and BREAKS for breaking bad news were major concerns among participants.ConclusionThe scoping review reveals both positive and negative experiences of breaking bad news by health professionals in the WHO’s Eastern Mediterranean Region. Most studies highlight the need for culturally sensitive targeted education and training programs on breaking bad news. Further research, particularly using qualitative methodologies and theoretical frameworks is warranted.
Two decades of progress in glioma methylation research: the rise of temozolomide resistance and immunotherapy insights
Authors: Huo Xianhao , Li Haoyuan , Xing Yixiang , Liu Wenqing , Chen Pengfei , Du Fang , Song Lijuan , Yu Zhenhua , Cao Xiangmei , Tian Jihui 
Published: September 2024

AimsThis study aims to systematically analyze the global trends in glioma methylation research using bibliometric methodologies. We focus on identifying the scholarly trajectory and key research interests, and we utilize these insights to predict future research directions within the epigenetic context of glioma.MethodsWe performed a comprehensive literature search of the Web of Science Core Collection (WoSCC) to identify articles related to glioma methylation published from January 1, 2004, to December 31, 2023. The analysis included full-text publications in the English language and excluded non-research publications. Analysis and visualization were performed using GraphPad Prism, CiteSpace, and VOSviewer software.ResultsThe search identified 3,744 publications within the WoSCC database, including 3,124 original research articles and 620 review articles. The research output gradually increased from 2004 to 2007, followed by a significant increase after 2008, which peaked in 2022. A minor decline in publication output was noted during 2020–2021, potentially linked to the coronavirus disease 2019 pandemic. The United States and China were the leading contributors, collectively accounting for 57.85% of the total research output. The Helmholtz Association of Germany, the German Cancer Research Center (DKFZ), and the Ruprecht Karls University of Heidelberg were the most productive institutions. The Journal of Neuro-Oncology led in terms of publication volume, while Neuro-Oncology had the highest Impact Factor. The analysis of publishing authors revealed Michael Weller as the most prolific contributor. The co-citation network analysis identified David N. Louis's article as the most frequently cited. The keyword analysis revealed “temozolomide,” “expression,” “survival,” and “DNA methylation” as the most prominent keywords, while “heterogeneity,” “overall survival,” and “tumor microenvironment” showed the strongest citation bursts.ConclusionsThe findings of this study illustrate the increasing scholarly interest in glioma methylation, with a notable increase in research output over the past two decades. This study provides a comprehensive overview of the research landscape, highlighting the importance of temozolomide, DNA methylation, and the tumor microenvironment in glioma research. Despite its limitations, this study offers valuable insights into the current research trends and potential future directions, particularly in the realm of immunotherapy and epigenetic editing techniques.