bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proceedings of the 1st biannual bridging the gaps in lung cancer conference
Authors: Florez Narjust , Patel Sandip P , Wakelee Heather , Bazhenova Lyudmila , Massarelli Erminia , Salgia Ravi , Stiles Brendon , Peters Solange , Malhotra Jyoti , Gadgeel Shirish M , Nieva Jorge J , Afkhami Michelle , Hirsch Fred R , Gubens Matthew , Cascone Tina , Levy Benjamin , Sabari Joshua , Husain Hatim , Ma Patrick C , Backhus Leah M , Iyengar Puneeth , Lee Percy , Miller Russell , Sands Jacob , Kim Edward 
Published: September 2024

Abstract
Lung cancer is the leading cause of cancer death in the US and globally. The mortality from lung cancer has been declining, due to a reduction in incidence and advances in treatment. Although recent success in developing targeted and immunotherapies for lung cancer has benefitted patients, it has also expanded the complexity of potential treatment options for health care providers. To aid in reducing such complexity, experts in oncology convened a conference (Bridging the Gaps in Lung Cancer) to identify current knowledge gaps and controversies in the diagnosis, treatment, and outcomes of various lung cancer scenarios, as described here. Such scenarios relate to biomarkers and testing in lung cancer, small cell lung cancer, EGFR mutations and targeted therapy in non-small cell lung cancer (NSCLC), early-stage NSCLC, KRAS/BRAF/MET and other genomic alterations in NSCLC, and immunotherapy in advanced NSCLC.
Minimized Dark Consumption of Calvin Cycle Intermediates Facilitates the Initiation of Photosynthesis in Synechocystis sp. PCC 6803
Authors: Tanaka Kenya , Kondo Akihiko , Hasunuma Tomohisa 
Published: September 2024

Abstract
Cyanobacteria intricately regulate their metabolic pathways during the diurnal cycle to ensure survival and growth. Under dark conditions, the breakdown of glycogen, an energy reserve, in these organisms replenishes Calvin cycle intermediates, especially downstream glycolytic metabolites, which are necessary for photosynthesis initiation upon light irradiation. However, it remains unclear how the accumulation of these intermediates is maintained in the dark despite limited glycogen availability. Therefore, in this study, we investigated the regulation of downstream glycolytic metabolites of the Calvin cycle under dark and light conditions using Synechocystis sp. PCC 6803. Our results showed that during the dark period, low pyruvate kinase (Pyk) activity ensured metabolite accumulation, while endogenous Pyk overexpression significantly lowered the accumulation of glycolytic intermediates. Remarkably, wild-type Synechocystis maintained oxygen evolution ability throughout dark treatment for over 2 d, while Pyk overexpression resulted in decreased oxygen evolution after 16 h of dark treatment. These results indicated that limiting Pyk activity via darkness treatment facilitates photosynthetic initiation by maintaining glycolytic intermediates. Similarly, phosphoenolpyruvate carboxylase (PepC) overexpression decreased oxygen evolution under dark treatment; however, its effect was lower than that of Pyk. Furthermore, we noted that as PepC overexpression decreased the levels of glycolytic intermediates in the dark, sugar phosphates in the Calvin–Benson–Bassham (CBB) cycle showed high accumulation, suggesting that sugar phosphates play important roles in supporting photosynthesis initiation. Therefore, our study highlights the importance of controlling the metabolic pathways through which glycolytic and CBB cycle intermediates are consumed (defined as cataplerosis of the CBB cycle) to ensure stable photosynthesis.
Neck or Shoulder? Establishing Consensus for Spine Screening in Patients With Shoulder Pain: An International Modified Delphi Study
Authors: Requejo-Salinas Néstor , Fernández-Matías Rubén , Cadogan Angela , Chester Rachel , Roy Jean-Sébastien , Struyf Filip , Bateman Marcus , Balster Simon , Haik Melina Nevoeiro , Seitz Amee L , Bisset Leanne , Camargo Paula Rezende , Brismée Jean-Michel , May Stephen , Walker Tom , Wassinger Craig , Lenssen Ross , Powell Jared K , McCreesh Karen , Gibson Jo , Ludewig Paula M , La Touche Roy , Lluch-Girbés Enrique 
Published: September 2024

ABSTRACT

Objective
There is no established consensus for screening the spine in patients with shoulder pain. The aim of this study was to explore the role of the spine in shoulder pain and generate a set of recommendations for assessing the potential involvement of the spine in patients with shoulder pain.

Methods
A modified Delphi study was conducted through use of an international shoulder physical therapist’s expert panel. Three domains (clinical reasoning, history, and physical examination) were evaluated using a Likert scale, with consensus defined as Aiken Validity Index ≥0.7.

Results
Twenty-two physical therapists participated. Consensus was reached on a total of 30 items: clinical reasoning (n = 9), history (n = 13), and physical examination (n = 8). The statement that spinal and shoulder disorders can coexist, sometimes influencing each other and at other times remaining independent issues, along with the concept of radiating pain as an explanatory phenomenon for the spine contribution to shoulder pain, achieved the highest degree of consensus.

Conclusion
International physical therapists shoulder experts reached consensus on key aspects when screening the spine in people with shoulder pain, including consideration of the distal location of symptoms relative to the shoulder, the presence or previous history of neck pain, the changes in symptoms related to neck movements, and the presence of neuropathic-like symptoms. They also acknowledged the importance of assessing active cervical or cervicothoracic movements and the usefulness of the Spurling test and symptom modification techniques applied to the spine.

Impact
This consensus holds implications for both clinical practice and research. In research, applying these considerations may ensure more homogenous samples, thereby enhancing the investigation of outcomes in shoulder pain populations. In clinical practice, determining the need for spine screening and its potential impact on prognosis and management could significantly influence patient care.
Arteriovenous Shunts: Their Location and Role in Physiology, Pathology, and Tissue Transfer. A Preliminary Report in the Upper Limb
Authors: Taylor G. Ian , Gascoigne Adam , Dodwell Prue , Corlett Russell , Pribaz Julian , Ramakrishnan Anand , Bruechert Georga , Fogg Quentin 
Published: September 2024

Background:
Tiny arteriovenous (AV) shunts of 10–150 µm (0.01–0.15 mm) are documented in the hands and feet. Larger shunts up to 0.5 mm (500 µm) have been discovered by the authors in the inner canthus and the human eye. This study seeks their possible existence in the upper limb.

Methods:
Radiographic lead oxide cadaver injection and dissection studies of 14 archival and six new upper limbs were examined.

Results:
AV shunts of 0.1–0.5 mm were discovered between the brachial, ulnar, and radial arteries and their venae comitantes and between their arterial perforators and the subcutaneous veins.

Conclusion:
This pilot study provides insight into the possible function of these large AV shunts associated with blood flow variation in temperature, blood pressure, tissue transfer, flap prefabrication, and flap necrosis.
Treatment of Facial Artery Embolization Caused by Polycaprolactone-based Dermal Filler with a Regimen Including 5-Fluorouracil
Authors: Guo Fengfeng , Xia Yuxi , Wei Qingqian , Zhuang Jun , Li Jinge , Hu Jintian 
Published: September 2024

Summary:
Polycaprolactone-based fillers are commonly used in plastic surgery to improve facial aging. However, adverse vascular events following these injections have been reported. An arterial embolism is a rare but serious complication associated with injectable filler procedures. We report a case of arterial embolism in a 35-year-old woman who received a polycaprolactone-based dermal filler at the nasal base and was treated with a regimen containing 5-fluorouracil. We discuss the potential causes of the patient’s condition and assess the superiority of our method over conventional approaches. Before treatment, ultrasound detected a decreased flow velocity in the patient’s right medial canthus artery with distal hypoperfusion, which improved significantly posttreatment. Long-term follow-up revealed near-disappearance of facial scarring. Our treatment effectively addressed facial artery embolism without any reported adverse reactions.
Comparing Cortiva Silhouette to AlloDerm for Use in Prepectoral Two-stage Prosthetic Breast Reconstruction
Authors: Silverstein Max L. , Wan Derrick , Momeni Arash 
Published: September 2024

Background:
The use of acellular dermal matrices (ADMs) in implant-based breast reconstruction has become increasingly routine during the past 20 years. ADMs improve soft-tissue support, facilitate greater tissue expander (TE) fill volumes, and reduce rates of capsular contracture. As the ADM market continues to grow, outcomes studies are necessary to assess the risks and benefits of each product. In this study, we compare the performance of Cortiva Silhouette, the thinnest ADM widely available, to AlloDerm, commonly considered the industry standard.

Methods:
We performed a retrospective review of 178 consecutive two-stage prosthetic breast reconstructions performed by the senior author. In every case, either Cortiva or AlloDerm was used to provide soft-tissue support during TE placement. Subjects were divided into Cortiva and AlloDerm cohorts and compared across patient characteristics and reconstructive outcomes variables.

Results:
During the study period, AlloDerm was used in 116 reconstructions; Cortiva was used in 62. After propensity score matching (62 AlloDerm, 62 Cortiva), Cortiva was associated with greater intraoperative and final TE fill volumes, as well as larger silicone implants. Cortiva was also associated with fewer complications overall, and fewer instances of mastectomy skin necrosis, delayed wound healing, and seroma.

Conclusions:
Cortiva Silhouette is noninferior to AlloDerm in terms of safety and providing soft-tissue support in prepectoral two-stage implant-based breast reconstruction. In this study, Cortiva supported greater TE fill volumes and larger silicone implants relative to AlloDerm and was associated with fewer complications.
Treatment of Eyelid Entropion: A Historical Review
Authors: Custer Philip L. 
Published: September 2024

Purpose:
The purpose of this study was to review the long history of entropion, with emphasis on its significance to the specialty of ophthalmology, and the numerous methods developed to treat cicatricial and involutional forms of the condition.

Methods:
An extensive medical literature review was performed, identifying publications containing information on entropion and associated conditions. Foreign language manuscripts were translated using online resources.

Results:
A total of 920 manuscripts published between 1505 and 2023 were reviewed. While the majority were written in English, 38.5% were foreign language works. For much of history, both cicatricial and involutional entropion were managed by shortening the anterior lamella. Horizontal eyelid margin or posterior tarsal incisions were often added for cicatricial disease. Later, different tissues were grafted into the defects created by these incisions. A variety of measures were used to temporize in patients with involutional entropion. Surgical treatment of this condition evolved to include methods of suture rotation, orbicularis weakening or redirection, tarsal stabilization, lid margin shortening, and eyelid retractor repair.

Conclusions:
Entropion and its management are described throughout recorded history. For much of this time, cicatricial entropion was the most common form of the disease, in great part secondary to the prevalence of trachoma. The social impact of these conditions was a primary factor in the establishment of ophthalmology as a medical specialty and the creation of dedicated eye hospitals. Publications describing the treatment of involutional entropion appear with increasing frequency after the mid-19th century. Arguably, more procedures have been described for entropion than for any other ophthalmic condition.
Use of Geological Maps in Detecting Asbestos-Related Diseases; A New Region in Anatolia
Authors: Ogun Hamza , Kansu Abdullah , Kansu Zeynep Eğri , Bayram Mehmet 
Published: September 2024

Objective
To examine the potential relationship between the presence of asbestos-related diseases (ARDs) in the region of Kastamonu, Turkey.

Methods
The places of birth of patients with ARDs and control subjects diagnosed between 2008 and 2019 and identified in a tertiary hospital in Istanbul. Soil samples were taken from plaster surfaces and quarries. The analysis was done with transmission electron microscopy.

Results
ARDs was detected in 55(17.9%) of 307 participants. Patients had a mean age of 68 ± 11 years. Residential proximity to ophiolites increased ARD incidence by 6.2% per kilometer (P = 0.003). Birthplaces were identified as being inside an ophiolitic unit, or if they were not, the Google Earth software was used to determine the beeline distance between the settlement's center and the edge of the closest ophiolitic unit. The appropriate threshold for this case is 12.75 km, with 75% sensitivity and 87% specificity.

Conclusion
ARDs due to naturally occurring asbestos (NOA) are present in hitherto unknown places. Geological maps including ophiolites can help locate these places.
Predicting adherence to ankle-foot orthoses in people with stroke: An application of the Theory of Planned Behavior
Authors: McMonagle Christine , Rasmussen Susan , Rooney Robbie , Elliott Mark A 
Published: September 2024

Background:
Ankle-foot orthoses (AFOs) are often prescribed for people with stroke to address motor impairment. However, not all patients adhere to using their AFOs as prescribed. Predictors of AFO adherence are likely to constitute useful targets for interventions that aim to promote this behavior.

Objective:
To identify the predictors of AFO adherence in people with stroke using an established theoretical framework, namely the Theory of Planned Behavior (TPB; Ajzen, 1991).

Study design:
Prospective survey design.

Method:
N = 49 people who had been prescribed an AFO after a stroke completed a TPB questionnaire. One month later, they completed another questionnaire that measured adherence to AFOs as prescribed by an orthotic practitioner. Predictors of intention and AFO-adherence behavior were identified using regression analyses.

Results
Adherence to AFOs was 63%. The TPB accounted for 61% of the variance in intentions and 43% of the variance in AFO-adherence behavior. Attitude and perceived behavioral control were predictors of intention, and intention was the sole predictor of behavior.

Conclusion
The TPB is a useful model for explaining adherence to AFOs in people with stroke. Interventions could be designed to increase intentions to use an AFO as prescribed by promoting positive attitudes towards, and perceived behavioral control of AFO adherence.
<xhtml:span xmlns:xhtml="http://www.w3.org/1999/xhtml" xml:lang="en">A comparative genomic and phylogenomic study of Nocardia seriolae strains unveils high genetic homogeneity and open&#160;nature of the pangenome
</xhtml:span>
Authors: Islam Sk Injamamul , Shahed Khandker , Ahamed Md Imtiaz , Mohammed Haitham 
Published: September 2024

The pathogen Nocardia seriolae is responsible for causing chronic granulomatous disease, known as fish nocardiosis, which has been observed in more than 40 species of fish cultured in marine and freshwater environments. Therefore, it is imperative to perform research to address and eradicate this substantial threat to the aquaculture industry. However, quantifying the negative impacts associated with this species is difficult because of insufficient data and underreporting. While several genomes are available in online databases, there is still a lack of comparative genomic analyses and studies on effective treatments, which is particularly important for aquaculture. To gain a deeper understanding of the pathogenic mechanisms associated with various Nocardia strains causing disease outbreaks and their virulence components, as well as the functional enrichment and genetic diversity during infection, it is crucial to develop a dependable and replicable pangenomic dataset. This study quantified Nocardia pangenome openness and discovered the biological processes that drive it. The study also found a substantial association between pangenome openness and numerous parameters such as genomic plasticity, gene synteny, single-copy phylogenomics, and pangenome prediction. The results showed genetic similarity across strains, indicating that the pangenome of this species is moving toward completeness. The diversity was supported by the similarity analysis, which revealed a high percentage of genes in the core genome and close association among the strains. Our findings provide valuable information and emphasize the necessity of sequencing novel genomes of N. serioale isolates to gain a deeper understanding of their diversity and patterns of adaptability.
Nsp1 stalls DNA Polymerase α at DNA hairpins
Authors: Baranovskiy Andrey G. , Morstadt Lucia M. , Babayeva Nigar D. , Tahirov Tahir H. 
Published: September 2024

AbstractThe human primosome, a four-subunit complex of DNA primase and DNA polymerase alpha (Polα), plays a critical role in DNA replication by initiating RNA and DNA synthesis on both chromosome strands. A recent study has shown that a major virulence factor in the SARS-CoV-2 infection, Nsp1 (non-structural protein 1), forms a stable complex with Polα but does not affect the primosome activity. Here we show that Nsp1 inhibits DNA synthesis across inverted repeats prone to hairpin formation. Analysis of current structural data revealed the overlapping binding sites for Nsp1 and the winged helix-turn-helix domain of RPA (wHTH) on Polα, indicating a competition between them. Comparison of the inhibitory effect of Nsp1 and wHTH on DNA hairpin bypass by Polα showed an 8-fold lower IC50value for Nsp1 (1 µM). This study provides a valuable insight into the mechanism of inhibition of human DNA replication by Nsp1 during a SARS-CoV-2 infection.
Differential effects of obesity and diabetes on the action potential waveform and inward currents in rat ventricular myocytes
Authors: Shmygol Anatoliy , Bru-Mercier Gilles , Sultan Ahmed S. , Howarth Frank C. 
Published: September 2024

AbstractObesity is a significant health concern worldwide, increasing the risk for type 2 diabetes mellitus (T2DM) and cardiovascular disease. Studies have found various vascular anomalies, abnormal heart rhythm, and impaired electro-mechanical coupling in patients with diabetes. Research on non-diabetic obese individuals has shown that besides diabetes-related complications, obesity itself raises the risk of developing cardiovascular disease. Recent studies have revealed a decrease in the speed of electrical signal conduction in the heart, along with slight gap junction dysfunction, which is insufficient to explain the observed impediment of impulse conduction. It’s still unclear whether this impairment is due to obesity-related fat toxicity or diabetes-related factors. Our study aimed to investigate the ventricular action potential parameters and voltage-gated Na+(INa) and Ca2+(I(Ca, L)) currents in Zucker fatty (ZF) rats in comparison to Zucker diabetic fatty (ZDF) rats, a well-established model of obesity and T2DM.Ventricular myocytes were enzymatically isolated from 25-30-week-old Zucker rats. Resting and action potentials were recorded from isolated left ventricular myocytes using a β-escin perforated patch clamp in current-clamp mode; INaand I(Ca, L)were recorded using whole-cell patch clamp techniques.Ventricular myocytes from ZF rats showed higher excitability and faster upstroke velocity. ZF rats also had a larger density of INa. Conversely, ZDF rats had decreased INawhich correlated with a reduced velocity of the action potential upstroke. There were no changes in the density or voltage sensitivity of I(Ca, L)among the three groups of animals.In conclusion, obesity alone and obesity accompanied by DM have distinct effects on the action potential waveform, INadensity and excitability of ventricular myocytes in a rat model of T2DM.
IL-6 and IL-27 negatively regulate CRTAM-expressing CD4+T-cells associated with lymphoid-driven synovitis
Authors: Derrac Soria Alicia , Lewis Myles , Liu Xiao , Twohig Jason P , Monaco Federica , Dimonte Sandra , Cardus Figueras Ana , Morrin Aisling , Guy Carol , Cossins Benjamin C , Benson Robert , Andrews Robert , Choy Ernest H , Garside Paul , Huwaikem Muneerah , Stemmler Marc P , Brabletz Simone , Brabletz Thomas , Siebzehnrubl Florian , Rodrigues Neil P. , Jenkins Brendan J. , Pitzalis Costantino , Jones Gareth W , Jones Simon A 
Published: September 2024

ABSTRACTJoint pathology in rheumatoid arthritis is heterogeneous, with histology providing evidence of fibroblast-driven, myeloid-driven, and lymphoid-driven synovitis. However, the immuno-modulatory pathways underlying their development remain unclear. Profiling synovial tissues from rheumatoid arthritis patients and mice with antigen-induced arthritis, we identified a subset of synovial infiltrating CD4+T-cells expressing CRTAM (class-I MHC-restricted T-cell-associated molecule). In human synovial biopsies,CRTAMcorrelated with the expression of effector cytokines (IL21,IFNG), chemokine receptors (CXCR3,CXCR4,CCR5), granzymes (GZMA,GZMB,GZMK), and regulatory factors (TIGIT,EOMES,BATF) linked with T-cell-mediated immunity. Studies of antigen-induced arthritis showed that CRTAM+CD4+T-cells accumulate in the inflamed synovium following disease onset. CRTAM+CD4+T-cells were particularly abundant in synovial tissue fromIl27ra-/-mice displaying ectopic lymphoid-like structures. CADM1 (cell adhesion molecule-1), the endogenous ligand for CRTAM, was also expressed in human synovitis and synovial tissues from wild-type,Il6ra-/-, andIl27ra-/-mice with antigen-induced arthritis. Cells expressing human CADM1 included synovial fibroblasts and subsets of monocytic and CD19+cells. Considering theex vivoregulation of CRTAM, we identified that activation of naïve CD4+T-cell increased CRTAM expression. This induction was blocked by IL-6 and IL-27, with further studies identifying a role for STAT3 in controlling the CRTAM transcriptional repressor, ZEB1. These results provide insights into the cytokine control of CRTAM on CD4+T-cells and support the involvement of CRTAM+CD4+T-cells in lymphoid-driven synovitis.