bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium falciparum dipeptidyl aminopeptidase 3 activity is important for efficient erythrocyte invasion by the malaria parasite
Authors: Lehmann Christine , Tan Michele Ser Ying , Vries Laura E. de , Russo Ilaria , Sanchez Mateo Isidrio , Goldberg Dan E. , Deu Edgar 
Published: October 2017

ABSTRACTParasite egress from infected erythrocytes and invasion of new erythrocytes are essential for the exponential asexual replication of the malaria parasite, and both processes are regulated and mediated by proteases. The putative cysteine protease dipeptidyl aminopeptidase 3 (DPAP3) was previously suggested to be essential for parasite egress, but little is known about its biological function. Here, we demonstrate that DPAP3 has proteolytic activity, but contrary to previously studied DPAPs, removal of its prodomain is not required for activation. Interestingly, P. falciparum DPAP3 localizes to merozoite apical organelles from which it is secreted immediately before egress. Using a conditional knock out approach coupled to complementation studies with wild type or mutant DPAP3, we show that DPAP3 activity is critical for efficient RBC invasion and overall parasite replication, and demonstrate that it does not play a role in parasite egress. Overall, this study establishes DPAP3 as a key regulator of erythrocyte invasion.
HYPO: A database of hypothetical human proteins
Authors: Sundararajan Vijayaraghava Seshadri , Malik Girik , Ijaq Johny , Kumar Anuj , Das Partha Sarathi , P.R Shidhi , Nair Achuthsankar S , Suravajhala Prashanth , Dhar Pawan K 
Published: October 2017

ABSTRACTAll annotated genes were once hypothetical or uncharacterized. Keeping this as an epilogue, we have enhanced our former database of hypothetical proteins (HP) in human (HypoDB) with added annotation, application programming interfaces and descriptive features. The database hosts 1000+ manually curated records of the known ‘unknown’ regions in the human genome. The new updated version of HypoDB with functionalities (Blast, Match) is freely accessible at http://www.bioclues.org/hypo2.
TPXL-1 Activates Aurora A to Clear Contractile Ring Components from the Polar Cortex During Cytokinesis
Authors: Mangal Sriyash , Sacher Jennifer , Kim Taekyung , Osório Daniel Sampaio , Motegi Fumio , Carvalho Ana Xavier , Oegema Karen , Zanin Esther 
Published: October 2017

ABSTRACTDuring cytokinesis, a signal from the bundled microtubules that form between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular components of the aster-based inhibitory signal, we developed a means to monitor the removal of contractile ring proteins from the polar cortex after anaphase onset. Using this assay, we show that polar clearing is an active process that requires activation of Aurora A kinase by TPXL-1. TPXL-1 concentrates on astral microtubules coincident with polar clearing in anaphase, and its ability to recruit Aurora A and activate its kinase activity are essential for clearing. In summary, our data identify Aurora A kinase as an aster-based inhibitory signal that restricts contractile ring components to the cell equator during cytokinesis.SUMMARYDuring cytokinesis, centrosomal asters inhibit cortical contractility at the cell poles. Mangal et al. provide molecular insight into this phenomenon, showing that TPXL-1, which localizes to astral microtubules, activates Aurora A kinase to clear contractile ring proteins from the polar cortex.
Addressing confounding artifacts in reconstruction of gene co-expression networks
Authors: Parsana Princy , Ruberman Claire , Jaffe Andrew E. , Schatz Michael C. , Battle Alexis , Leek Jeffrey T. 
Published: October 2017

AbstractBackgroundGene co-expression networks capture diverse biological relationships between genes, and are important tools in predicting gene function and understanding disease mechanisms. Functional interactions between genes have not been fully characterized for most organisms, and therefore reconstruction of gene co-expression networks has been of common interest in a variety of settings. However, methods routinely used for reconstruction of gene co-expression networks do not account for confounding artifacts known to affect high dimensional gene expression measurements.ResultsIn this study, we show that artifacts such as batch effects in gene expression data confound commonly used network reconstruction algorithms. Both theoretically and empirically, we demonstrate that removing the effects of top principal components from gene expression measurements prior to network inference can reduce false discoveries, especially when well annotated technical covariates are not available. Using expression data from the GTEx project in multiple tissues and hundreds of individuals, we show that this latent factor residualization approach often reduces false discoveries in the reconstructed networks.ConclusionNetwork reconstruction is susceptible to confounders that affect measurements of gene expression. Even controlling for major individual known technical covariates fails to fully eliminate confounding variation from the data. In studies where a wide range of annotated technical factors are measured and available, correcting gene expression data with multiple covariates can also improve network reconstruction, but such extensive annotations are not always available. Our study shows that principal component correction, which does not depend on study design or annotation of all relevant confounders, removes patterns of artifactual variation and improves network reconstruction in both simulated data, and gene expression data from GTEx project. We have implemented our PC correction approach in the Bioconductor package sva which can be used prior to network reconstruction with a range of methods.
The correspondence problem: which brain maps are significantly similar?
Authors: Alexander-Bloch Aaron , Vandekar Simon N. , Shinohara Russell T. , Liu Siyuan , Satterthwaite Theodore D. , Glahn David C. , Raznahan Armin 
Published: October 2017

ABSTRACTA critical issue in many neuroimaging studies is the comparison between brain maps. How should we test the hypothesis that two or more brain maps are partially convergent or overlap to a significant extent? This “correspondence problem” affects, for example, the interpretation of comparisons between task-based patterns of functional activation, resting-state networks or modules, and neuroanatomical landmarks. In published work, this problem has been addressed with remarkable variability in terms of methodological approaches and statistical rigor. In this paper, we address the correspondence problem using a spatial permutation framework to generate null models of overlap, by applying random rotations to spherical representations of the cortical surface. We use this approach to derive clusters of cognitive functions that are significantly similar in terms of their functional neuroatomical substrates. In addition, using publicly available data, we formally demonstrate the correspondence between maps of task-based functional activity, resting-state fMRI networks and gyral-based anatomical landmarks. We provide open-access code to implement the methods presented for two commonly-used tools for surface based cortical analysis. This spatial permutation approach constitutes a useful advance over widely-used methods for the comparison of cortical maps, and thereby opens up new possibilities for the integration of diverse neuroimaging data.
High-throughput single-cell DNA sequencing of AML tumors with droplet microfluidics
Authors: Pellegrino Maurizio , Sciambi Adam , Treusch Sebastian , Durruthy-Durruthy Robert , Gokhale Kaustubh , Jacob Jose , Chen Tina X. , Oldham William , Matthews Jairo , Kantarjian Hagop , Futreal P. Andrew , Patel Keyur , Jones Keith W. , Takahashi Koichi , Eastburn Dennis J. 
Published: October 2017

ABSTRACTTo enable the characterization of genetic heterogeneity in tumor cell populations, we developed a novel microfluidic approach that barcodes amplified genomic DNA from thousands of individual cancer cells confined to droplets. The barcodes are then used to reassemble the genetic profiles of cells from next generation sequencing data. Using this approach, we sequenced longitudinally collected AML tumor populations from two patients and genotyped up to 62 disease relevant loci across more than 16,000 individual cells. Targeted single-cell sequencing was able to sensitively identify tumor cells during complete remission and uncovered complex clonal evolution within AML tumors that was not observable with bulk sequencing. We anticipate that this approach will make feasible the routine analysis of heterogeneity in AML leading to improved stratification and therapy selection for the disease.
Residential placement and quality of life for adults with severe autism spectrum disorders and severe-to-profound intellectual disabilities
Authors: Galli Carminati Giuliana , Carminati Federico , Lehotkay Rachel , Lorincz Erica Nora , Subirade-Jacopit Viviane , Rondini Elisa , Bertelli Marco O. 
Published: October 2017

PurposeThe purpose of this paper is to evaluate the relationship between some main characteristics of different living arrangements and the quality of life (QoL) of their users with severe intellectual disability and low-functioning autism spectrum disorders.Design/methodology/approachStudy participants were assessed for ASD severity through the Childhood Autism Rating Scale or the Vineland Adaptive Behavior Scales (VABS): for behavioral problems with the aberrant behavior checklist (ABC); for perception of efficacy and satisfaction with care, through an adapted Visual Analogue Scale; and for QoL with the QoL inventory in residential environments (validated in French as Inventaire de la Qualité de Vie en Milieu Résidentiel). Because the goal was to define a “residential profile (RP),” the authors evaluated each participating residence with the Working Methods Scale and the questionnaire on residential parameters.FindingsThe RP allowed for the classification of the residences into three clusters. The authors found no clear relationship between QoL and the RP clusters, but the authors found the RP clusters to be significantly correlated with ABC factors F1 (irritability, agitation, crying) and F2 (lethargy, social withdrawal), and VABS scores for living, socialization, and motor skills.Originality/valueRPs were more strongly correlated with ABC items and the ability to cope with everyday life than with QoL. The authors hypothesize that RP is correlated with both aberrant behavior and the autonomy of residents and that QoL remains relatively stable. Therefore, RP is correlated with the status of the residents; however, this appears not to be correlated with their QoL.
Using push-pull winescape attributes to model Australian wine tourist segmentation
Authors: Quintal Vanessa , Thomas Ben , Phau Ian , Soldat Zorana 
Published: October 2017

Purpose
The study aims to introduce a comprehensive segmentation instrument that incorporates the push–pull winescape attributes, providing a new perspective of the wine tourist profile and explaining their behavioural intentions in the Australian winescape.

Design/methodology/approach
A literature review, focus groups and expert panels generated an extensive list of push–pull winescape attributes. Pen-and-paper surveys conveniently sampled 739 wine tourists at three wineries across three wine regions in Australia. Adopting push–pull winescape attributes as the segmentation base, cluster analysis identified four segments, namely, inspireds, self-drivens, market-drivens and inerts, and their behavioural intentions were examined.

Findings
Inspireds demonstrate both self- and market-motivation. Self-drivens exhibit self-motivation but limited market-motivation, whereas Market-drivens characterise market-motivation but limited self-motivation. Inerts are limited in both market- and self-motivations. At the Swan Valley, all four segments were identified, with Inspireds being the most willing to revisit and recommend to others and Inerts, the least willing. At the Barossa Valley, only two segments emerged. Again, Inspireds and Inerts were the most and least willing to revisit and recommend to others respectively. Finally, at the Yarra Valley, three segments were identified. Market-drivens were most willing to revisit and recommend to others, followed by self-drivens and lastly, by inerts.

Research limitations/implications
A comprehensive push–pull winescape segmentation base of wine tourists is introduced, which provides a more sophisticated profile of wine tourist segments than otherwise would be attained with conventional measures.

Practical implications
New insights into who the wine tourist is and what it is they seek from the winescape are vital to smaller wine producers whose best access to the domestic retail and export markets is through direct selling at the cellar door.

Originality/value
The empirically tested 18-item push–pull winescape instrument presents a comprehensive segmentation approach, which profiles wine tourists and predicts their behavioural intentions based on an extensive investigation of push–pull winescape attributes.
Strategic profiling and the value of wine & tourism initiatives
Authors: Dressler Marc 
Published: October 2017

PurposeThis paper aims to contribute to the growing body of literature on wine and tourism, as it explores the value contribution of tourism in the context of strategic grouping of wineries.Design/methodology/approachA panel of three online surveys about the strategic management of German wine estates served to analyze wine and tourism as potential strategic levers. More than 300 German wineries participated in the surveys.FindingsAlmost 50 per cent of the interviewed wineries planned to extend their tourism services. The implementation rate is high and reaches nearly the level of new product implementation. Tourism is therefore highly relevant. It can serve to profile in the market. Cost leaders and boutique wineries were two strategic groups indicating reluctance to pursue tourism-based strategic initiatives. The data illustrate a potential strategic trade-off between tourism and export management.Research limitations/implicationsThe study is neither representative for the German nor for an international winery population. Data were generated in a broader context of strategic and innovation management research. Descriptive analyses dominate the explorative study.Practical implicationsExtending services to win tourists helps to differentiate and to attract new clients. For less differentiated strategies (price-value and quality-leadership), tourism can be a strategic lever to sustainably increase profits. Smaller wineries need to strategically assess their growth option. They may face a strategic dilemma whether to export their goods or to add tourism offer components. Fierce international competition and restrictions due to small size represent export barriers, favoring a strategy to exploit market potentials via tourism. Concerted wine tourism efforts require a more sensitive approach considering the individual strategic motivation of wineries. The strategic value depends on the strategic grouping.Social implicationsWine and tourism can create regional and thereby social value. Addressing the strategic value of tourism for the individual wineries fosters tourism engagement and encourages a cluster approach.Originality/valueLiterature universally praises the synergetic value of wine and tourism. This research proposes a more sensitive approach reflecting strategic groupings and individual value contribution of tourism activities for the wine estates.
The relationship between involvement, destination emotions and place attachment in the Porto wine cellars
Authors: Santos Vasco Ribeiro , Ramos Paulo , Almeida Nuno 
Published: October 2017

Purpose
This paper aims to measure the role of involvement, destination emotions and place attachment in the behavioural intentions of wine tourists when visiting Porto wine cellars.

Design/methodology/approach
An interceptive survey was conducted with wine tourists during their visits to four Porto wine cellars. A convenience sample of 918 international visitors was obtained. A structural equation model using partial least squares analysis was used to test the hypothesis and the validity of the constructs and model.

Findings
The structural model results indicated that wine tourists’ personal involvement and their wine involvement have a significant and direct influence on destination emotions and place attachment in Porto wine cellars during the visits, which determines their future behavioural intentions.

Research limitations/implications
The generalizability of the results may be limited owing to the sample and the data collection method and the short time frame of the data gathering.

Practical implications
There is a growing potential for researchers and managers to achieve benefits from the proposed model that will support the efforts for wine tourism theory and practice, such as brand positioning strategies and formulation of sharper marketing strategies.

Originality/value
This is the first study to demonstrate the combined use of personal and wine involvement on destination emotions along with place attachment in a wine tourist behaviour context. This approach extends the scope into a wine tourism context because the combination of these three constructs has never been held in the area of wine tourism destinations.
Online retailers connecting to the youth segment through Facebook (A study on the influence of gender and personality type)
Authors: Mishra Oly , Ayatham Prasad 
Published: October 2017

Purpose
The purpose of this paper is to develop an understanding of the different personality types and Facebook behavior of the young Indian consumers in the context of e-tailing. With the increased popularity of Facebook, consumers and e-tailers are using it as a platform to connect better with each other. This paper makes an attempt to study the motive and online purchasing behavior of youth by examining Facebook posts and understanding their relationship with personality and gender.

Design/methodology/approach
A survey was conducted on 200 young Indian consumers who use Facebook as well as follow at least one e-tailer on Facebook. The responses to the survey were used to test the proposed conceptual model to find out the relationship of personality types and gender with Facebook behavior, type of post liked on Facebook, reason to use Facebook and motive to follow an e-tailer on Facebook using descriptive statistics and nonparametric tests.

Findings
The tests showed that gender of online consumers influences their Facebook behavior and the type of post that they like the most on Facebook. The personality type of the online consumers has a significant influence on the motive to follow an e-tailer on Facebook and the reason to use Facebook. It implies that the Facebook pages of e-tailers should plan their posts keeping the gender and personality type of their followers in mind. In this manner, Facebook can be used as a platform by the e-tailers to increase their consumer base, especially among the youth.

Research limitations/implications
The limitations of this study are the respondents for this study are in the age group of 15-25 years only and the results of the study may vary with the change in the age group. The respondents are Indians by nationality and the results may vary for different nations.

Practical implications
This paper provides an insight to the e-tailer about attracting young consumers on their Facebook page in the context of their gender and personality type to enhance the reach and expand their business. This research paper is informative to the e-tailers who have budgets for social media marketing to know about social media usage by youth for online shopping.

Originality/value
Presently, India has a demographic advantage and this paper contributes to understand the youth social media usage for online shopping. The e-tailers can relate the findings to develop strategies to enhance their customer base. This research paper will contribute to the existing literature on social media marketing and e-tailing.
Impact of advertising strategies on the cognitive and behavioral component of attitude of women consumers
Authors: Kaur Harminderjit , Hundal Bikramjit Singh 
Published: October 2017

Purpose
The purpose of this paper is to cover the gap from the previous literature with regard to the cognitive and the behavioural component of attitude of consumers. The literature was scant in examining the influence of the mostly used traits on the purchase behaviour and the switching behaviour of consumers. Therefore, this research paper examines the impact of different marketing strategies used by the advertisers on the components of the attitude of the consumers.

Design/methodology/approach
First, the perception of the consumers towards the traits was measured by the factor analysis approach. Second, the impact of the traits on the purchase behaviour of consumers was scrutinized using regression analysis, and then descriptive statistics approach was used to analyse the switching behaviour and the most important tactic used in the advertisement.

Findings
The results indicate that repeated exposure, comparison of products and sexual appeals has a significant impact on the mind of consumers which determines the influence of advertising tactics. Further, the results evaluated that information, pricing element, image of company and sexual appeal are the other important traits influencing the cognitive attitude of consumers. The consumers switch to the advertised products which indicate the behavioural change with the impact of advertisement.

Research limitations/implications
This research paper is suffering from some limitations as the area of research is restrained to the urban population of three districts of Punjab only, i.e. Amritsar, Jalandhar and Ludhiana, because of which the results cannot be generalized for other areas. Due to the changing behaviour and the attitude, income level and media, the response of the consumers in the present study may not be relevant in the future period. The study was confined to the working women consumers only and does not represent the whole population.

Practical implications
This research paper provides an insight to the marketers. The managers can measure how by using appropriate tactics can they make their advertising more effective. In case of unfamiliar products, the attitude of consumers to accept or to avoid the product is influenced by the tactics used by the marketers. It was recommended that the marketers must use the appropriate tactics to make their products/brands more pertinent and important among the consumers.

Originality/value
This research paper examines the impact of TV advertisement of beauty products on the components of the attitude of the urban working women consumers. This study presents the influence of the various marketing strategies used in the advertisement to influence the purchase and the switching behaviour of consumers.