bluuebunny/crossref_metadata_2025_split huggingface dataset:
Critical Illness–Associated Cerebral Microbleeds
Authors: Fanou Evgenia M. , Coutinho Jonathan M. , Shannon Patrick , Kiehl Tim-Rasmus , Levi Marcel M. , Wilcox M. Elizabeth , Aviv Richard I. , Mandell Daniel M. 
Published: February 2017

Background and Purpose—
Cerebral microbleeds (petechial hemorrhages) are a well-known consequence of cerebral amyloid angiopathy and chronic hypertension among other causes. We report 12 patients with a clinically and radiologically distinct microbleed phenomenon in the cerebral white matter.

Methods—
These patients were assessed at the University Health Network (Toronto, Canada) between 2004 and 2014.

Results—
Median age was 40 years (range, 27–63 years), and 7 out of 12 patients were women. All patients had brain magnetic resonance imaging during or immediately after an intensive care unit admission. All patients had respiratory failure, 11 out of 12 received mechanical ventilation, and 3 out of 12 received extracorporeal life support. Magnetic resonance imaging in all 12 patients showed extensive microbleeds, diffusely involving the juxtacortical white matter and corpus callosum but sparing the cortex, deep and periventricular white matter, basal ganglia, and thalami. Several patients also had internal capsule or posterior fossa involvement.

Conclusions—
We have described a distinct microbleed phenomenon in the cerebral white matter of patients with critical illness. The specific cause of the microbleeds is unclear, but the pathogenesis may involve hypoxemia as the microbleeds are similar to those described with high-altitude exposure.
Nutrition Management of Home Parenteral Nutrition Among Patients With Enterocutaneous Fistula in the Sustain Registry
Authors: Compher Charlene , Winkler Marion F. , Guenter Peggi , Steiger Ezra 
Published: February 2017

AbstractBackground: Home parenteral nutrition (HPN) is a vital therapy for patients who have the diagnosis of enterocutaneous fistula (ECF), yet little is known about how these patients are managed. This research compares nutrition management of adults with ECF as the indication for HPN therapy to those with other indications. Methods: This is an analysis of data from adult HPN patients in the Sustain registry enrolled between August 2011 and February 2014 who have the diagnosis of ECF or other indication for HPN who served as the control group. Differences between the ECF and control group were assessed by t test, analysis of variance, or χ2 as appropriate. Results: There were 141 HPN patients with ECF and 632 control patients. Patients with ECF were older (55 vs 50 years, P < .001), more frequently had a goal for future surgery (30% vs 15%, P = .010), had greater prevalence of overweight/obesity (33% vs 20%, P = .04), and had a lower serum albumin (2.98 ± 0.65 g/dL vs 3.16 ± 0.66 g/dL, P = .006) than controls. The diet order was more frequently nil per os (NPO) in patients with ECF (48% vs 22%, P < .001), and amino acid content of HPN was greater (111.90 ± 29.11 vs 102.06 ± 27.84, P < .001) than in controls. There were no differences in patterns of weight change by ECF or control groups, although underweight patients gained, normal‐weight patients maintained, and overweight/obese patients lost weight and serum albumin increased similarly. Conclusions: The HPN management of patients with ECF is similar to other HPN patients other than greater provision of protein, more frequent NPO status, and a goal for future surgery.
Lipid Injectable Emulsions: Infusion Confusion
Authors: Ayers Phil , Boullata Joseph I. , Guenter Peggi , Holcombe Beverly 
Published: February 2017

AbstractThe commentary addresses discussion regarding the duration of infusion for lipid injectable emulsions (ILEs; previously known as intravenous fat emulsion) when administered separately from amino acids and dextrose (2:1) admixtures. The article by Mundi et al describes the administration time of ILEs administered separately as up to 24 hours, while previous American Society for Parenteral and Enteral Nutrition documents have outlined the infusion time not to exceed 12 hours.
Pulmonary hypertension and right heart failure due to severe hypernatremic dehydration
Authors: Chiwane Saurabh , Ahmed Tageldin M. , Bauerfeld Christian P. , Chauhan Monika 
Published: February 2017

Introduction: Neonates are at risk of developing hypernatremic dehydration and its associated complications, such as stroke, dural sinus thrombosis and renal vein thrombosis. Pulmonary hypertension has not been described as a complication of hypernatremia. Case report: We report a case of a seven-day-old neonate with severe hypernatremic dehydration who went on to develop pulmonary hypertension and right heart failure needing extracorporeal membrane oxygenation (ECMO). Discussion: Normal or high anion gap metabolic acidosis commonly accompanies hypernatremic dehydration. The presence of acidosis and/or hypoxia can delay the normal drop in pulmonary vascular resistance (PVR) after birth, causing pulmonary hypertension and right ventricular failure. Conclusion: A high index of suspicion is paramount to diagnose pulmonary hypertension and aggressive correction of the acidosis and hypoxia is needed. In the presence of severe right ventricular failure, ECMO can be used as a bridge to recovery while underlying metabolic derangements are being corrected.
Aiming at the same target: A masked priming study directly comparing derivation and inflection in the second language
Authors: Jacob Gunnar , Heyer Vera , Veríssimo João 
Published: February 2017

Aims and objectives/purpose/research questions: We compared the processing of morphologically complex derived vs. inflected forms in native speakers of German and highly proficient native Russian second language (L2) learners of German. Design/methodology/approach: We measured morphological priming effects for derived and inflected German words. To ensure that priming effects were genuinely morphological, the design also contained semantic and orthographic control conditions. Data and analysis: 40 native speakers of German and 36 native Russian learners of L2 German participated in a masked-priming lexical-decision experiment. For both participant groups, priming effects for derived vs. inflected words were compared using linear mixed effects models. Findings/conclusions: While first language (L1) speakers showed similar facilitation effects for both derived and inflected primes, L2 speakers showed a difference between the two prime types, with robust priming effects only for derived, but not for inflected forms. Originality: Unlike in previous studies investigating derivation and inflection in L2 processing, priming effects for derived and inflected prime–target pairs were determined on the basis of the same target word, allowing for a direct comparison between the two morphological phenomena. In this respect, this is the first study to directly compare the processing of derived vs. inflected forms in L2 speakers. Significance/implications: The results are inconsistent with accounts predicting general L1/L2 differences for all types of morphologically complex forms as well as accounts assuming that L1 and L2 processing are based on the same mechanisms. We discuss theoretical implications for L2 processing mechanisms, and propose an explanation which can account for the data pattern.
Development of platelets during steady state and inflammation
Authors: Müller-Newen Gerhard , Stope Matthias B , Kraus Thomas , Ziegler Patrick 
Published: February 2017

Abstract
Megakaryocytes (MK) are the sole source of platelets in the body. They develop from lineage-committed hematopoietic stem and progenitor cells (HSPCs) via intermediate cells, which differ in morphology, size, ploidy, and surface phenotype. Development and maturation of MKs is governed by different transcription factors, including GATA-1, E26 transformation-specific transcription factor (ETS) family members, nuclear factor erythroid 2 transcription factor (NF-E2), and STAT3. During such challenges as acute inflammation, platelets are consumed in high numbers and must be replenished to secure survival of the host. This is achieved by integration of inflammatory signals into early MK development and depends on the STAT1-mediated enhanced translation of transcripts in stem cell-like megakaryocyte progenitors. Here, we review recent developments, which highlight the impact of inflammation on the development of platelets from HSPCs.
TLR10 suppresses the activation and differentiation of monocytes with effects on DC-mediated adaptive immune responses
Authors: Hess Nicholas J , Felicelli Christopher , Grage Jennifer , Tapping Richard I 
Published: February 2017

Abstract
TLRs are important pattern-recognition receptors involved in the activation of innate immune responses against foreign pathogens. TLR10 is the only TLR family member without a known ligand, signaling pathway, or clear cellular function. Previous work has shown that TLR10 suppresses proinflammatory cytokine production in response to TLR agonists in a mixed human mononuclear cell population. We report that TLR10 is preferentially expressed on monocytes and suppresses proinflammatory cytokine production resulting from either TLR or CD40 stimulation. TLR10 engagement affects both the MAPK and Akt signaling pathways, leading to changes in the transcriptome of isolated human monocytes. Differentiation of monocytes into dendritic cells in the presence of an αTLR10 mAb reduced the expression of maturation markers and the induction of proinflammatory cytokines, again in response to either TLR or CD40 stimulation. Finally, in coculture experiments, TLR10 differentiated dendritic cells exhibited a decreased capacity to activate T cells as measured by IL-2 and IFN-γ production. These data demonstrate that TLR10 is a novel regulator of innate immune responses and of the differentiation of primary human monocytes into effective dendritic cells.
Frontline Science: Shh production and Gli signaling is activated in vivo in lung, enhancing the Th2 response during a murine model of allergic asthma
Authors: Standing Ariane S I , Yánez Diana C , Ross Rosie , Crompton Tessa , Furmanski Anna L 
Published: February 2017

Abstract
The pathophysiology of allergic asthma is driven by Th2 immune responses after aeroallergen inhalation. The mechanisms that initiate, potentiate, and regulate airway allergy are incompletely characterized. We have shown that Hh signaling to T cells, via downstream Gli transcription factors, enhances T cell conversion to a Th2 phenotype. In this study, we showed for the first time, to our knowledge, that Gli-dependent transcription is activated in T cells in vivo during murine AAD, a model for the immunopathology of asthma, and that genetic repression of Gli signaling in T cells decreases the differentiation and recruitment of Th2 cells to the lung. T cells were not the only cells that expressed activated Gli during AAD. A substantial proportion of eosinophils and lung epithelial cells, both central mediators of the immunopathology of asthma, also underwent Hh/Gli signaling. Finally, Shh increased Il-4 expression in eosinophils. We therefore propose that Hh signaling during AAD is complex, involving multiple cell types, signaling in an auto- or paracrine fashion. Improved understanding of the role of this major morphogenetic pathway in asthma may give rise to new drug targets for this chronic condition.
Dynamics of myeloid cell populations during relapse-preventive immunotherapy in acute myeloid leukemia
Authors: Rydstroüm Anna , Hallner Alexander , Aurelius Johan , Sander Frida Ewald , Bernson Elin , Kiffin Roberta , Thoren Fredrik Bergh , Hellstrand Kristoffer , Martner Anna 
Published: February 2017

Abstract
Relapse of leukemia in the postchemotherapy phase contributes to the poor prognosis and survival in patients with acute myeloid leukemia (AML). In an international phase IV trial (ClinicalTrials.gov; NCT01347996), 84 patients with AML in first complete remission who had not undergone transplantation received immunotherapy with histamine dihydrochloride (HDC) and low-dose IL-2 with the aim of preventing relapse. The dynamics of myeloid cell counts and expression of activation markers was assessed before and after cycles of immunotherapy and correlated with clinical outcome in terms of relapse risk and survival. During cycles, a pronounced increase in blood eosinophil counts was observed along with a reduction in monocyte and neutrophil counts. A strong reduction of blood monocyte counts during the first HDC/IL-2 treatment cycle predicted leukemia-free survival. The HDC component of the immunotherapy exerts agonist activity at histamine type 2 receptors (H2Rs) that are expressed by myeloid cells. It was observed that the density of H2 R expression in blood monocytes increased during cycles of immunotherapy and that high monocyte H2R expression implied reduced relapse risk and improved overall survival. Several other activation markers, including HLA-DR, CD86, and CD40, were induced in monocytes and dendritic cells during immunotherapy but did not predict clinical outcome. In addition, expression of HLA-ABC increased in all myeloid populations during therapy. A low expression of HLA-ABC was associated with reduced relapse risk. These results suggest that aspects of myeloid cell biology may impact clinical benefit of relapse-preventive immunotherapy in AML.
Inductive induced polarization effect in heliborne time-domain electromagnetic data for uranium exploration, northern part of Cuddapah Basin, India
Authors: Ramesh Babu Veldi , Patra Indrajit , Tripathi Shailesh , Muthyala Sridhar , Chaturvedi Anand K. 
Published: February 2017

The Peddagattu, Lambapur, Chitrial and Koppunuru uranium deposits along the northern margins of the Cuddapah Basin are confined to the middle Proterozoic unconformity interface between Archean basement granites and the overlying resistive quartzites. Negative transients observed in the coincident loop heliborne time-domain electromagnetic (HTEM) data over these deposits (occurring in outliers) are believed to be due to thick polarizable conductive zones occurring along the unconformity. Similar negative HTEM responses are recorded over the Gorukunta Tanda outlier. A ground spectral induced polarization (SIP) survey conducted over the outlier and ground geologic observations indicated an altered basement/regolith with thickness up to 5 m below 20–30 m thick quartzite. Interpretation of Cole-Cole parameters computed from the SIP data indicated a change in the dispersion. These Cole-Cole parameters were used in modeling negative HTEM data assuming a polarizable plate placed in a layered earth at a depth of approximately 50 m using the CSIRO LeroiAir program. A negative [Formula: see text] response in the late channels indicated that the negatives can be explained in terms of inductive induced polarization effects. Modeling of HTEM [Formula: see text] data for the profile through the Lambapur uranium deposit and the Gorukunta Tanda reveals the presence of a polarizable lithologic unit at a depth of approximately 40 m. This unit is interpreted as an argillic alteration of basement, with the presence of clay and/or disseminated sulfides as evidenced from the core extracted from the boreholes at depths below the highly resistive quartzite. Uranium mineralization is closely associated with altered basement and sulfides along the unconformity where the distinct negative electromagnetic (EM) signature is recorded. Furthermore, there exists a good correlation between the uranium mineralization grade and the thickness versus the averaged late-channel negative HTEM response over the known deposits. The negative EM response helped in locating the new target areas for uranium exploration.
Heterozygous Vangl2
Looptail mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair
Authors: Poobalasingam Thanushiyan , Yates Laura L. , Walker Simone A. , Pereira Miguel , Gross Nina Y. , Ali Akmol , Kolatsi-Joannou Maria , Jarvelin Marjo-Riitta , Pekkanen Juha , Papakrivopoulou Eugenia , Long David A. , Griffiths Mark , Wagner Darcy , Königshoff Melanie , Hind Matthew , Minelli Cosetta , Lloyd Clare M. , Dean Charlotte H. 
Published: February 2017

Lung diseases impose a huge economic and health burden worldwide. A key aspect of several adult lung diseases, such as Idiopathic pulmonary fibrosis (IPF) and Chronic Obstructive pulmonary Disease (COPD), including emphysema, is aberrant tissue repair, which leads to an accumulation of damage and impaired respiratory function. Currently, there are few effective treatments available for these diseases and their incidence is rising.
The Planar Cell Polarity (PCP) pathway is critical for the embryonic development of many organs, including kidney and lung. We have previously shown that perturbation of the PCP pathway impairs tissue morphogenesis, which disrupts the number and shape of epithelial tubes formed within these organs during embryogenesis. However, very little is known about the role of the PCP pathway beyond birth, partly due to the perinatal lethality of many PCP mouse mutant lines.
Here we have investigated heterozygous Looptail (Lp) mice, in which a single copy of the core PCP gene, Vangl2, is disrupted. We show that these mice are viable but display severe airspace enlargement and impaired adult lung function. Underlying these defects, we find that Vangl2Lp/+ lungs exhibit altered distribution of actin microfilaments and abnormal regulation of the actin modifying protein cofilin. In addition, we show that Vangl2Lp/+ lungs exhibit many of the hallmarks of tissue damage including an altered macrophage population, abnormal elastin deposition and elevated levels of the elastin-modifying enzyme, Mmp12, all of which are observed in the lung disease, emphysema.
In vitro, VANGL2 disruption impairs directed cell migration and reduces the rate of repair following scratch wounding of human alveolar epithelial cells. Moreover, using population data from a birth cohort of young adults, all aged 31, we found evidence of an interactive effect between VANGL2 and smoking (a tissue damaging insult) on lung function. Finally, we show that that PCP genes VANGL2 and SCRIBBLE (SCRIB) are significantly downregulated in lung tissue from patients with emphysema.
Our data reveals an important novel role for the PCP pathway in adult lung homeostasis and repair and sheds new light on the genetic factors which may modify destructive lung diseases such as emphysema.
The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking
Authors: Conkar Deniz , Culfa Efraim , Odabasi Ezgi , Rauniyar Navin , Yates John R. , Firat-Karalar Elif N. 
Published: February 2017

ABSTRACT
Centriolar satellites are membrane-less structures that localize and move around the centrosome and cilium complex in a microtubule-dependent manner. They play important roles in centrosome- and cilium-related processes, including protein trafficking to the centrosome and cilium complex, and ciliogenesis, and they are implicated in ciliopathies. Despite the important regulatory roles of centriolar satellites in the assembly and function of the centrosome and cilium complex, the molecular mechanisms of their functions remain poorly understood. To dissect the mechanism for their regulatory roles during ciliogenesis, we performed an analysis to determine the proteins that localize in close proximity to the satellite protein CEP72, among which was the retinal degeneration gene product CCDC66. We identified CCDC66 as a microtubule-associated protein that dynamically localizes to the centrosome, centriolar satellites and the primary cilium throughout the cell cycle. Like the BBSome component BBS4, CCDC66 distributes between satellites and the primary cilium during ciliogenesis. CCDC66 has extensive proximity interactions with centrosome and centriolar satellite proteins, and co-immunoprecipitation experiments revealed interactions between CCDC66, CEP290 and PCM1. Ciliogenesis, ciliary recruitment of BBS4 and centriolar satellite organization are impaired in cells depleted for CCDC66. Taken together, our findings identify CCDC66 as a targeting factor for centrosome and cilium proteins.
Non-coding Y RNAs associate with early replicating euchromatin in concordance with the origin recognition complex
Authors: Kheir Eyemen , Krude Torsten 
Published: February 2017

ABSTRACT
Non-coding Y RNAs are essential for the initiation of chromosomal DNA replication in vertebrates, yet their association with chromatin during the cell cycle is not characterised. Here, we quantify human Y RNA levels in soluble and chromatin-associated intracellular fractions and investigate, topographically, their dynamic association with chromatin during the cell cycle. We find that, on average, about a million Y RNA molecules are present in the soluble fraction of a proliferating cell, and 5–10-fold less are in association with chromatin. These levels decrease substantially during quiescence. No significant differences are apparent between cancer and non-cancer cell lines. Y RNAs associate with euchromatin throughout the cell cycle. Their levels are 2–4-fold higher in S phase than in G1 phase or mitosis. Y RNAs are not detectable at active DNA replication foci, and re-associate with replicated euchromatin during mid and late S phase. The dynamics and sites of Y1 RNA association with chromatin are in concordance with those of the origin recognition complex (ORC). Our data therefore suggest a functional role of Y RNAs in a common pathway with ORC.
Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin
Authors: Kazemi D. , Shams Asenjan K. , Dehdilani N. , Parsa H. 
Published: February 2017

ObjectivesMesenchymal stem cells have the ability to differentiate into various cell types, and thus have emerged as promising alternatives to chondrocytes in cell-based cartilage repair methods. The aim of this experimental study was to investigate the effect of bone marrow derived mesenchymal stem cells combined with platelet rich fibrin on osteochondral defect repair and articular cartilage regeneration in a canine model.MethodsOsteochondral defects were created on the medial femoral condyles of 12 adult male mixed breed dogs. They were either treated with stem cells seeded on platelet rich fibrin or left empty. Macroscopic and histological evaluation of the repair tissue was conducted after four, 16 and 24 weeks using the International Cartilage Repair Society macroscopic and the O’Driscoll histological grading systems. Results were reported as mean and standard deviation (sd) and compared at different time points between the two groups using the Mann-Whitney U test, with a value < 0.05 considered statistically significant.ResultsHigher cumulative macroscopic and histological scores were observed in stem cell treated defects throughout the study period with significant differences noted at four and 24 weeks (9.25, sd 0.5 vs 7.25, sd 0.95, and 10, sd 0.81 vs 7.5, sd 0.57; p < 0.05) and 16 weeks (16.5, sd 4.04 vs 11, sd 1.15; p < 0.05), respectively. Superior gross and histological characteristics were also observed in stem cell treated defects.ConclusionThe use of autologous culture expanded bone marrow derived mesenchymal stem cells on platelet rich fibrin is a novel method for articular cartilage regeneration. It is postulated that platelet rich fibrin creates a suitable environment for proliferation and differentiation of stem cells by releasing endogenous growth factors resulting in creation of a hyaline-like reparative tissue. Cite this article: D. Kazemi, K. Shams Asenjan, N. Dehdilani, H. Parsa. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin: Macroscopic and histological assessments. Bone Joint Res 2017;6:98–107. DOI: 10.1302/2046-3758.62.BJR-2016-0188.R1.