bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth inhibition by selenium is associated with changes in primary metabolism and nutrient levels in Arabidopsis thaliana
Authors: Ribeiro Dimas M. , Silva Júnior Dalton D. , Cardoso Flávio Barcellos , Martins Auxiliadora O. , Silva Welder A. , Nascimento Vitor L. , Araújo Wagner L. 
Published: June 2016

AbstractAlthough Selenium (Se) stress is relatively well known for causing growth inhibition, its effects on primary metabolism remain rather unclear. Here, we characterized both the modulation of the expression of specific genes and the metabolic adjustments in Arabidopsis thaliana in response to changes in Se level in the soil. Se treatment culminated with strong inhibition of both shoot and root growth. Notably, growth inhibition in Se‐treated plants was associated with an incomplete mobilization of starch during the night. Minor changes in amino acids levels were observed in shoots and roots of plants treated with Se whereas the pool size of tricarboxylic acid (TCA) cycle intermediates in root was not altered in response to Se. By contrast, decreased levels of organic acids involved in the first part of the TCA cycle were observed in shoots of Se‐treated plants. Furthermore, decreased expression levels of expansins and endotransglucosylases/endohydrolases (XHTs) genes were observed after Se treatment, coupled with a significant decrease in the levels of essential elements. Collectively, our results revealed an exquisite interaction between energy metabolism and Se‐mediated control of growth in Arabidopsis thaliana to coordinate cell wall extension, starch turnover and the levels of a few essential nutrients.
Pediatric Erythema Annulare Centrifugum Treated with Oral Fluconazole: A Retrospective Series
Authors: Kruse Lacey L. , Kenner‐Bell Brandi M. , Mancini Anthony J. 
Published: June 2016

AbstractObjectivesErythema annulare centrifugum (EAC) is an uncommon eruption that may be a hypersensitivity reaction. Treatment of EAC is best accomplished by eliminating the underlying cause. Although many triggers have been reported, the inciting factor is unknown in most patients. We hypothesized that occult yeast overgrowth may trigger EAC in patients with EAC of unknown etiology.MethodsFive children with EAC were treated empirically with oral fluconazole. These cases were retrospectively reviewed.ResultsImprovement was noted in all patients; three cleared entirely. Two patients experienced recurrences after successful treatment, which were treated with a second course of fluconazole. The treatment was well tolerated, without adverse effects.ConclusionOral fluconazole was an effective treatment in five children with EAC.
Behavioral and emotional responses to diet‐related support and control among same‐sex couples
Authors: AUGUST KRISTIN J. , ABBAMONTE JOHN M. , MARKEY CHARLOTTE N. , NAVE CHRISTOPHER S. , MARKEY PATRICK M. 
Published: June 2016

AbstractMembers of 63 lesbian and 72 gay couples reported how frequently their partners engaged in diet‐related social support and control (persuasion and pressure) and how they responded behaviorally and emotionally to such attempts. Although lesbian women received more frequent diet‐related social support than gay men, there were no gender differences in the receipt of diet‐related social control. Results of multilevel models that controlled for body mass index revealed that all participants responded to all types of involvement with guilt and to support and persuasion with appreciation. Responses to pressure differed for lesbian women and gay men and acted as a function of relationship quality. Findings from this study may increase awareness of the importance of same‐sex partners' involvement in eating behaviors.
A functional classification of medial frontal negativity ERPs: Theta oscillations and single subject effects
Authors: Van Noordt Stefon J.R. , Campopiano Allan , Segalowitz Sidney J. 
Published: June 2016

AbstractTheta oscillations in the EEG have been linked to several ERPs that are elicited during performance‐monitoring tasks, including the error‐related negativity (ERN), no‐go N2, and the feedback‐related negativity (FRN). We used a novel paradigm to isolate independent components (ICs) in single subjects' (n = 27) EEG accounting for a medial frontal negativity (MFN) to response cue stimuli that signal a potential change in future response demands. Medial frontal projecting ICs that were sensitive to these response cues also described the ERNs, no‐go N2s, and, to a lesser extent, the FRNs, that were elicited in letter flanker, go/no‐go, and time‐estimation tasks, respectively. In addition, percentile bootstrap tests using trimmed means indicated that the medial frontal ICs show an increase in theta activity during the ERN, no‐go N2, and FRN across tasks and within individuals. Our results provide an important validation of previous studies by showing that increases in medial frontal theta to cognitively challenging events in multiple paradigms is a reliable effect within individuals and can be elicited by basic stimulus cues that signal the potential need to adjust response control. Thus, medial frontal theta reflects a neural response common to all MFN paradigms and characterizes the general process of controlling attention without the need to induce error commission, inhibited responses, or to present negative feedback.
Causal Analysis of Ordinal Treatments and Binary Outcomes Under Truncation by Death
Authors: Wang Linbo , Richardson Thomas S. , Zhou Xiao-Hua 
Published: June 2016

Summary
It is common that, in multiarm randomized trials, the outcome of interest is ‘truncated by death’, meaning that it is only observed or well-defined conditioning on an intermediate outcome. In this case, in addition to pairwise contrasts, the joint inference for all treatment arms is also of interest. Under a monotonicity assumption we present methods for both pairwise and joint causal analyses of ordinal treatments and binary outcomes in the presence of truncation by death. We illustrate via examples the appropriateness of our assumptions in different scientific contexts.
Zoophycos in deep‐sea sediments indicates high and seasonal primary productivity: Ichnology as a proxy in palaeoceanography during glacial–interglacial variations
Authors: Dorador Javier , Wetzel Andreas , Rodríguez‐Tovar Francisco J. 
Published: June 2016

AbstractTrace fossils provide valuable palaeoenvironmental information in hemipelagic settings. This is particularly true in the case of Zoophycos, an easily recognizable trace fossil in core. At IODP site U1385, Zoophycos was found throughout an interval representing 1.5 Ma, covering 45 glacial–interglacial cycles mediated by obliquity (41 ka) and short‐term eccentricity (100 ka). Zoophycos is most common in sediments deposited during glacial times and when the sedimentation rate was intermediate and primary production was high and seasonal. Occurrences of Zoophycos elsewhere support a similar relationship with seasonal organic‐matter deposition. This is particularly significant considering that seasonality of organic‐matter deposition is difficult to decipher from the sediment record. Zoophycos appears to represent a useful proxy to characterize high and seasonal organic‐matter deposition and primary productivity in Neogene hemipelagic deposits.
Draft Genome Sequence of Comamonas thiooxydans Strain PHE2-6 (NBRC 110656), a Chlorinated-Ethene-Degrading Bacterium
Authors: Shimodaira Jun , Yonezuka Kenta , Tabata Michiro , Nagase Shun , Kasai Daisuke , Hosoyama Akira , Yamazoe Atsushi , Fujita Nobuyuki , Fukuda Masao 
Published: June 2016

ABSTRACT

Comamonas thiooxydans
strain PHE2-6 (NBRC 110656), which was isolated from a trichloroethene-contaminated site in Japan, utilizes phenol as a sole source of carbon and cometabolizes
cis-
and
trans-
dichloroethenes. We report here the draft genome sequence of this strain, containing 5,309,680 bp, with 60.6% G+C content.
Draft Genome Sequence and Gene Annotation of the Uropathogenic Bacterium Proteus mirabilis Pr2921
Authors: Giorello F. M. , Romero V. , Farias J. , Scavone P. , Umpiérrez A. , Zunino P. , Sotelo Silveira J. R. 
Published: June 2016

ABSTRACT

Here, we report the genome sequence of
Proteus mirabilis
Pr2921, a uropathogenic bacterium that can cause severe complicated urinary tract infections. After gene annotation, we identified two additional copies of
ucaA
, one of the most studied fimbrial protein genes, and other fimbriae related-proteins that are not present in
P. mirabilis
HI4320.
Complete Genome Sequence of the Methanogen Methanoculleus bourgensis BA1 Isolated from a Biogas Reactor
Authors: Maus Irena , Wibberg Daniel , Winkler Anika , Pühler Alfred , Schnürer Anna , Schlüter Andreas 
Published: June 2016

ABSTRACT

Methanoculleus bourgensis
BA1, a hydrogenotrophic methanogen
,
was isolated from a laboratory-scale biogas reactor operating under an elevated ammonium concentration. Here, the complete genome sequence of
M. bourgensis
BA1 is reported. The availability of the BA1 genome sequence enables detailed comparative analyses involving other
Methanoculleus
spp. representing important members of microbial biogas communities.
Genome Sequences of Gordonia terrae Phages Benczkowski14 and Katyusha
Authors: Pope Welkin H. , Benczkowski Matthew S. , Green Daryn E. , Hwang Melina , Kennedy Bryan , Kocak Bradley , Kruczek Ellen , Lin Leon , Moretti Matthew L. , Onelangsy Faith L. , Mezghani Nadia , Milliken Katherine A. , Toner Chelsea L. , Thompson Paige K. , Ulbrich Megan C. , Furbee Emily C. , Grubb Sarah R. , Warner Marcie H. , Montgomery Matthew T. , Garlena Rebecca A. , Russell Daniel A. , Jacobs-Sera Deborah , Hatfull Graham F. 
Published: June 2016

ABSTRACT

Bacteriophages Katyusha and Benczkowski14 are newly isolated phages that infect
Gordonia terrae
3612. Both have siphoviral morphologies with isometric heads and long tails (500 nm). The genomes are 75,380 bp long and closely related, and the tape measure genes (9 kbp) are among the largest to be identified.
High-Quality Draft Genome Sequence of Biocontrol Strain
Pantoea
sp. OXWO6B1
Authors: Town Jennifer , Audy Patrice , Boyetchko Susan M. , Dumonceaux Tim J. 
Published: June 2016

ABSTRACT

Pantoea
sp. strain OXWO6B1 inhibits the growth of the potato pathogen
Phytophthora infestans
. We determined the 5.2-Mbp genome sequence of this strain, which featured at least 3 confirmed plasmids of up to 250 kbp. The genome sequence of OXWO6B1 is different from that of all previously sequenced strains of
Pantoea
.
Complete Genome Sequence of a Multidrug-Resistant Acinetobacter baumannii Isolate Obtained from a Mexican Hospital (Sequence Type 422)
Authors: Castro-Jaimes Semiramis , Salgado-Camargo Abraham David , Graña-Miraglia Lucía , Lozano Luis , Bocanegra-Ibarias Paola , Volkow-Fernández Patricia , Silva-Sanchez Jesus , Castillo-Ramírez Santiago , Cevallos Miguel A. 
Published: June 2016

ABSTRACT

Acinetobacter baumannii
has emerged as a dangerous nosocomial pathogen, particularly for severely ill patients in intensive care units and patients with hematologic malignancies. Here, we present the complete genome sequence of a multidrug-resistant
A. baumannii
isolate, recovered from a Mexican hospital and classified as sequence type 422 according to the multilocus sequence typing Pasteur scheme.
High-Quality Draft Genome Sequence of Bacillus subtilis Strain WAUSV36
Authors: Town Jennifer , Audy Patrice , Boyetchko Susan M. , Dumonceaux Tim J. 
Published: June 2016

ABSTRACT

Bacillus subtilis
strain WAUSV36 inhibits the growth of and decreases disease symptoms caused by the potato pathogen
Phytophthora infestans
. We determined the sequence of the 4.7-Mbp genome of this strain. WAUSV36 shared very high nucleotide sequence identity with previously sequenced strains of
B. subtilis
.
Complete Genome Sequences of T1-Like Phages JMPW1 and JMPW2
Authors: Shen Mengyu , Zhu Hongbin , Lu Shuguang , Le Shuai , Li Gang , Tan Yinling , Zhao Xia , Shen Wei , Hu Fuquan , Wang Jing 
Published: June 2016

ABSTRACT

We report the complete genome sequences of phages JMPW1 (49,840 bp) and JMPW2 (50,298 bp), two T1-like
Escherichia coli
phages isolated from contaminated experiment samples. Although the genomes of JMPW1 and JMPW2 share high identity with T1, they show some differences, which are mainly located in several genes with unknown functions and genes encoding tail fiber proteins and endonucleases.
Complete Genome Sequence of the First KPC-Type Carbapenemase-Positive Proteus mirabilis Strain from a Bloodstream Infection
Authors: Di Pilato Vincenzo , Chiarelli Adriana , Boinett Christine J. , Riccobono Eleonora , Harris Simon R. , D'Andrea Marco Maria , Thomson Nicholas R. , Rossolini Gian Maria , Giani Tommaso 
Published: June 2016

ABSTRACT

Sequencing of the
bla
KPC
-positive strain
Proteus mirabilis
AOUC-001 was performed using both the MiSeq and PacBio RS II platforms and yielded a single molecule of 4,272,433 bp, representing the complete chromosome. Genome analysis showed the presence of several acquired resistance determinants, including two copies of
bla
KPC-2
carried on a fragment of a KPC-producing plasmid previously described in
Klebsiella pneumoniae
.
Draft Genome Sequence of Shewanella baltica M1 Isolated from Brackish Surface Water of the Gulf of Gdańsk
Authors: Karczewska-Golec Joanna , Strapagiel Dominik , Sadowska Marta , Szalewska-Pałasz Agnieszka , Golec Piotr 
Published: June 2016

ABSTRACT

Here, we present the 5.168-Mbp draft genome sequence of
Shewanella baltica
M1, the first
Shewanella
strain from the Gulf of Gdańsk to have its genome sequenced and annotated. The availability of the genome sequence of strain M1 will promote further global analyses of bacterial stress responses in the unique Gulf of Gdańsk ecosystem.
Draft Genome Sequences of Methanobrevibacter curvatus DSM11111, Methanobrevibacter cuticularis DSM11139, Methanobrevibacter filiformis DSM11501, and Methanobrevibacter oralis DSM7256
Authors: Poehlein Anja , Seedorf Henning 
Published: June 2016

ABSTRACT

Here, the draft genome sequences of four different
Methanobrevibacter
species are presented. Three of the
Methanobrevibacter
species (
M. curvatus, M. cuticularis
, and
M. filiformis
) have been isolated from the termite hindgut, while
M. oralis
was isolated from human subgingival plaque.
Improving the Standards-Based Management-Recognition initiative to provide high-quality, equitable maternal health services in Malawi: an implementation research protocol
Authors: Mumtaz Zubia , Salway Sarah , Nyagero Josephat , Osur Joachim , Chirwa Ellen , Kachale Fannie , Saunders Duncan 
Published: June 2016

BackgroundThe Government of Malawi is seeking evidence to improve implementation of its flagship quality of care improvement initiative—the Standards Based Management-Recognition for Reproductive Health (SBM-R(RH)).ObjectiveThis implementation study will assess the quality of maternal healthcare in facilities where the SBM-R(RH) initiative has been employed, identify factors that support or undermine effectiveness of the initiative and develop strategies to further enhance its operation.MethodsData will be collected in 4 interlinked modules using quantitative and qualitative research methods. Module 1 will develop the programme theory underlying the SBM-R(RH) initiative, using document review and in-depth interviews with policymakers and programme managers. Module 2 will quantitatively assess the quality and equity of maternal healthcare provided in facilities where the SBM-R(RH) initiative has been implemented, using the Malawi Integrated Performance Standards for Reproductive Health. Module 3 will conduct an organisational ethnography to explore the structures and processes through which SBM-R(RH) is currently operationalised. Barriers and facilitators will be identified. Module 4 will involve coordinated co-production of knowledge by researchers, policymakers and the public, to identify and test strategies to improve implementation of the initiative.Potential impactThe research outcomes will provide empirical evidence of strategies that will enhance the facilitators and address the barriers to effective implementation of the initiative. It will also contribute to the theoretical advances in the emerging science of implementation research.
Measuring engagement in advance care planning: a cross-sectional multicentre feasibility study
Authors: Howard Michelle , Bonham Aaron J , Heyland Daren K , Sudore Rebecca , Fassbender Konrad , Robinson Carole A , McKenzie Michael , Elston Dawn , You John J 
Published: June 2016

ObjectivesTo assess the feasibility, acceptability and clinical sensibility of a novel survey, the advance care planning (ACP) Engagement Survey, in various healthcare settings.SettingA target sample of 50 patients from each of primary care, hospital, cancer care and dialysis care settings.ParticipantsA convenience sample of patients without cognitive impairment who could speak and read English was recruited. Patients 50 and older were eligible in primary care; patients 80 and older or 55 and older with clinical markers of advanced chronic disease were recruited in hospital; patients aged 19 and older were recruited in cancer and renal dialysis centres.OutcomesWe assessed feasibility, acceptability and clinical sensibility of the ACP Engagement Survey using a 6-point scale. The ACP Engagement Survey measures ACP processes (knowledge, contemplation, self-efficacy and readiness) on 5-point Likert scales and actions (yes/no).Results196 patients (38–96 years old, 50.5% women) participated. Mean (±SD) time to administer was 48.8±19.6 min. Mean acceptability scores ranged from 3.2±1.3 in hospital to 4.7±0.9 in primary care, and mean relevance ranged from 3.5±1.0 in hospital to 4.9±0.9 in dialysis centres (p<0.001 for both). The mean process score was 3.1±0.6 and the mean action score was 11.2±5.6 (of a possible 25).ConclusionsThe ACP Engagement Survey demonstrated feasibility and acceptability in outpatient settings but was less feasible and acceptable among hospitalised patients due to length. A shorter version may improve feasibility. Engagement in ACP was low to moderate.
An explorative study to assess the association between health-related quality of life and the recommended phase II dose in a phase I trial: idarubicin-loaded beads for chemoembolisation of hepatocellular carcinoma
Authors: Anota Amélie , Boulin Mathieu , Dabakuyo-Yonli Sandrine , Hillon Patrick , Cercueil Jean-Pierre , Minello Anne , Jouve Jean-Louis , Paoletti Xavier , Bedenne Laurent , Guiu Boris , Bonnetain Franck 
Published: June 2016

ObjectivesThe objective of this study was to explore the association between health-related quality of life (HRQoL) and the recommended phase 2 dose in a phase I clinical trial according to the Time to HRQoL deterioration approach (TTD).SettingThis is a phase I dose-escalation trial of transarterial chemoembolisation (TACE) with idarubicin-loaded beads performed in cirrhotic patients with hepatocellular carcinoma. Patients had to complete the EORTC QLQ-C30 HRQoL questionnaire at baseline and at days 15, 30 and 60 after TACE.ParticipantsPatients aged ≥18 years with HCC unsuitable for curative treatments were evaluated for the study (N=21).Primary and secondary outcome measurementsThe primary objective was to determine the maximum tolerated dose (MTD) of idarubicin loaded after a single TACE session. MTD was defined as the dose level closest to that causing dose-limiting toxicity in 20% of patients. HRQoL was the secondary end point.ResultsBetween March 2010 and March 2011, 9, 6 and 6 patients were included at idarubicin dose levels of 5, 10 and 15 mg, respectively. Calculated MTD of idarubicin was 10 mg. At the 10 mg idarubicin dose, patients presented a longer TTD than at 5 mg, for global health status (HR=0.91 (95% CI 0.18 to 4.72)), physical functioning (HR=0.38 (0.04 to 3.22)), fatigue (HR=0.67 (0.18 to 2.56)) and pain (HR=0.47 (0.05 to 4.24)).ConclusionsThese HRQoL results were consistent with the estimated MTD, with a median TTD for global health status of 41 days (21 to NA) at 5 mg, 23 days (20 to NA) at 10 mg and 25 days (17 to NA) at 15 mg. These results show the importance of studying HRQoL in phase I trials.Trial registration numberNCT01040559; Post-results.
“You don't immediately stick a label on them”: a qualitative study of influences on general practitioners' recording of anxiety disorders
Authors: Ford Elizabeth , Campion Alice , Chamles Darleen Aixora , Habash-Bailey Haniah , Cooper Maxwell 
Published: June 2016

ObjectivesAnxiety is a common condition usually managed in general practice (GP) in the UK. GP patient records can be used for epidemiological studies of anxiety as well as clinical audit and service planning. However, it is not clear how general practitioners (GPs) conceptualise, diagnose and document anxiety in these records. We sought to understand these factors through an interview study with GPs.SettingUK National Health Service (NHS) General Practice (England and Wales).Participants17 UK GPs.Primary and secondary outcome measuresSemistructured interviews used vignettes to explore the process of diagnosing anxiety in primary care and investigate influences on recording. Interviews were transcribed verbatim and analysed using thematic analysis.ResultsGPs chose 12 different codes for recording anxiety in the 2 vignettes, and reported that history, symptoms and management would be recorded in free text. GPs reported on 4 themes representing influences on recording of anxiety: ‘anxiety or a normal response’, ‘granularity of diagnosis’, ‘giving patients a label’ and ‘time as a tool’; and 3 themes about recording in general: ‘justifying the choice of code’, ‘usefulness of coding’ and ‘practice-specific pressures’. GPs reported using only a regular selection of codes in patient records to help standardise records within the practice and as a time-saving measure.ConclusionsWe have identified a coding culture where GPs feel confident recognising anxiety symptoms; however, due to clinical uncertainty, a long-term perspective and a focus on management, they are reluctant to code firm diagnoses in the initial stages. Researchers using GP patient records should be aware that GPs may prefer free text, symptom codes and other general codes rather than firm diagnostic codes for anxiety.