bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights on mining the pangenome of Sphingobacterium thalpophilum NMS02 S296 from the resistant banana cultivar Pisang lilin confirms the antifungal action against Fusarium oxysporum f. sp. cubense
Authors: Ajesh B. R. , Sariga R. , Nakkeeran S. , Renukadevi P. , Saranya N. , Alkahtani Saad 
Published: September 2024

IntroductionFusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), poses a significant global threat to banana cultivation. Conventional methods of disease management are increasingly challenged, thus making it necessary to explore alternative strategies. Bacterial endophytes, particularly from resistant genotypes, are gaining attention as potential biocontrol agents. Sphingobacterium thalpophilum, isolated from the resistant banana cultivar Pisang lilin (JALHSB010000001-JALHSB010000029), presents an intriguing prospect for combating Fusarium wilt. However, its underlying biocontrol mechanisms remain poorly understood. This study aimed to elucidate the antifungal efficacy of S. thalpophilum NMS02 S296 against Foc and explore its biocontrol mechanisms at the genomic level.MethodsWhole genome sequencing of S. thalpophilum NMS02 S296 was conducted using next-generation sequencing technologies and bioinformatics analyses were performed to identify genes associated with antifungal properties. In vitro assays were used to assess the inhibitory effects of the bacterial isolate on the mycelial growth of Foc. To explore the biomolecules responsible for the observed antagonistic activity, metabolites diffused into the agar at the zone of inhibition between Foc S16 and S. thalpophilum NMS02 S296 were extracted and identified.ResultsWhole genome sequencing revealed an array of genes encoding antifungal enzymes and secondary metabolites in S. thalpophilum NMS02 S296. In vitro experiments demonstrated significant inhibition of Foc mycelial growth by the bacterial endophyte. Comparative genomic analysis highlighted unique genomic features in S. thalpophilum linked to its biocontrol potential, setting it apart from other bacterial species.DiscussionThe study underscores the remarkable antifungal efficacy of S. thalpophilum NMS02 S296 against Fusarium wilt. The genetic basis for its biocontrol potential was elucidated through whole genome sequencing, shedding light on the mechanisms behind its antifungal activity. This study advanced our understanding of bacterial endophytes as biocontrol agents and offers a promising avenue for plant growth promotion towards sustainable strategies to mitigate Fusarium wilt in banana cultivation.
No genetic causal relationship between acne and prostate cancer through Mendelian randomization combined with meta-analysis
Authors: He Mingyang , Wang Xin , Wang Dongbin , Wang Yaxuan , Qi Jinchun , Jia Jianghua , Zhang Ming , Meng Qingsong , Yan Bowen , Guo Heyang , Qu Changbao 
Published: September 2024

BackgroundPrevious observational studies regarding the relationship between acne and prostate cancer have reported inconsistent results. As such studies are prone to biases, we conducted this Mendelian randomization (MR) analysis to better explore the causal association between acne and prostate cancer.MethodsThe genetic data for assessing acne were acquired from the largest genome-wide association study (GWAS) of acne by far, and the genetic data for assessing prostate cancer were acquired from the FinnGen consortium, UK Biobank, European Bioinformatics Institute, and IEU OpenGWAS project. We performed two-sample MR analyses using data from these GWASs followed by a meta-analysis to provide an overall evaluation. The primary MR methods used included inverse variance weighted, MR-Egger, and weighted median. Leave-one-out sensitivity tests, Cochran’s Q tests, and MR-Egger intercept tests were used to bolster the robustness of the MR results.ResultsThrough MR combined with meta-analysis, our study found no genetic causal relationship between acne and prostate cancer (p=0.378; odds ratio=0.985; 95% confidence interval, 0.954–1.018). Sensitivity tests ensured the robustness of this result.ConclusionAcne should not be considered as a morbidity hazard factor for prostate cancer.
Fertility preservation in Malaysian pediatric cohort: a survey of healthcare providers’ knowledge, practice, attitude, perceptions and barriers
Authors: Ali Anizah , Lee Yew Kong , Alias Hamidah , Zainuddin Ani Amelia 
Published: September 2024

IntroductionImpaired future fertility potential secondary to gonadotoxic therapies for childhood cancer is a shattering aftermath faced by childhood cancer survivors. Fertility preservation (FP) has emerged as a key to mitigate this unwelcomed sequelae. FP services catering to the needs of children and adolescents (C&amp;A) population in developing countries are limited. Malaysia recently launched its pioneering pediatrics FP services.Aims of studyTo evaluate healthcare providers’ (HCPs) FP knowledge, practice behaviors, attitudes, perceptions, and barriers towards FP counseling/services (C/S) for the C&amp;A cohort.MethodsA questionnaire-based study was conducted utilizing a questionnaire consisting of 51 items which was adapted from G.Quinn et al. The questionnaire was distributed both online and physically amongst HCPs in a tertiary center. Ethical committee approval was granted by the Research Ethical Committee, Universiti Kebangsaan Malaysia.ResultsA total of 102 HCPs completed the questionnaires. The majority of respondents were Malays (74.5%), females (80.4%), gynecology/pediatrics specialty (76.5%), and had children (88.2%). Nearly 72% of HCPs demonstrated good knowledge of FP. Almost 73% of HCPs consulted reproductive specialists (RES) on potential fertility issues and over 80% of HCPs referred patients who enquired on fertility issues to RES. Only 17% of HCPs practiced FP discussion, 12% reported no available person to discuss FP, and 10% of HCPs were unaware of who to discuss FP with. Patients’ inability to afford FP (30.4%) tops the list of barriers to FP C/S, followed by limited available information on FP for patients (17.6%) and patients too ill to delay treatment (12.7%). Most HCPs (88.2%) demonstrated unfavorable attitudes towards FP C/S.DiscussionsIn general, the majority of our HCP respondents demonstrated good current FP knowledge and practice behaviors. Mitigating several controversial issues in FP would improve HCPs’ attitude towards FP. Main barriers to the uptake of FP C/S for C&amp;A were patient and resource barriers. Addressing these issues by funding aid for FP procedures, increasing FP knowledge dispersion, as well as developing age-appropriate FP-related educational materials would improve FP service provision for C&amp;A in the future.ConclusionsIn conclusion, successful corrective action combined with strategic planning points to a promising future for Malaysia's FP services provision for C&amp;A.
Comparative safety analysis of lacosamide and perampanel in epilepsy management: insights from FAERS database
Authors: Ge Chang , Jin Liuyin , Tian Jing-Jing , Yang Na , Xu Jian 
Published: September 2024

BackgroundEpilepsy is a chronic neurological condition requiring effective management with minimal adverse effects. Lacosamide (LCM) and Perampanel (PER), two promising treatments, have distinct profiles that merit comparative analysis to guide clinical decision-making.MethodsThis study utilizes a pharmacovigilance analysis of adverse events reported in the FDA Adverse Event Reporting System database from Q1 2009 to Q3 2023. Employing disproportionality and Bayesian analyses, we assessed and compared the AE signals associated with LCM and PER to elucidate their safety profiles in epilepsy treatment.ResultsThe analysis included 12,576 AE reports for LCM and 2,703 for PER, highlighting a higher incidence of psychiatric disorders, including aggression with LCM, and a notable association of PER with psychiatric disorders such as psychotic disorders and dizziness. LCM showed a relatively safe profile during pregnancy, whereas PER’s data suggested caution due to reported cases of suicidal ideation and attempts.ConclusionThis comprehensive evaluation underscores the importance of understanding the distinct AE profiles of LCM and PER in clinical practice, providing valuable insights for personalized epilepsy management. Future research with rigorous prospective designs is recommended to validate these findings and explore the mechanisms underlying the reported adverse events.
Exogenous Uniconazole promotes physiological metabolism and grain yield of rice under salt stress
Authors: Du Xiaole , Du Youwei , Feng Naijie , Zheng Dianfeng , Zhou Hang , Huo Jingxin 
Published: September 2024

IntroductionSalt stress severely inhibit plant growth and development. Uniconazole has been considered to significantly increase plant stress tolerance. However, the mechanism by which Uniconazole induces salt tolerance in rice seedlings and its impact on yield is still unclear.MethodsIn this study, the effects of exogenous Uniconazole on morphogenesis, physiological metabolism, and yield of rice seedlings under salt stress were analyzed using the salt-tolerant rice variety HD961 and the salt-sensitive rice variety 9311.ResultsThe results showed that salt stress significantly inhibited rice growth, disrupted the antioxidant system and pigment accumulation, and reduced photosynthesis, and yield. There were corresponding percent decreases of 13.0% and 24.1% in plant height, 31.6% and 55.8% in leaf area, 65.7% and 85.3% in root volume, respectively for HD961 and 9311. spraying However, compared to salt stress, the US treatment increased the percentage to 4.7% and 139.0% in root volume, 7.5% and 38.0% in total chlorophyll, 4.5% and 14.3% in peroxidase (POD) of leaves, 14.4% and 54.2% in POD of roots, 18.7% and 22.7% in catalase (CAT) of leaves, and 22.6% and 53.9% in CAT of roots, respectively, for HD961 and 9311. In addition, it also significantly enhanced photosynthesis at the reproductive stage, promoted the transport of carbohydrate to grains. And US treatment significantly increased the percentage to 9.0% in panicle length, 28.0% in panicle number per hole, 24.0% in filled grain number, 3.0% in 1000-grain weight, and 26.0% in yield per plant, respectively, for HD961, compared to salt stress.DiscussionIn summary, applying Uniconazole at the seedling stage can alleviate the damage induced by NaCl stress on rice by regulating the physiological metabolism of rice plants. This reduces the negative effects of salt stress, enhance salt tolerance, and boost rice production.
Development and validation of the Transgender Adolescent Stress Survey-Dysphoria
Authors: Schrager Sheree M. , Goldbach Jeremy T. , Wood Jules K. , O'Brien Rory P. , Dunlap Shannon L. , Rhoades Harmony 
Published: September 2024

ObjectiveTransgender and nonbinary adolescents (TNBA) may experience gender dysphoria arising from incongruities between their body and their gender. Prior dysphoria measures have largely focused on clinical diagnosis with little regard to comparability of forms for people assigned male or female at birth, overall psychometric performance, or applicability to nonbinary populations. This study develops and validates the Transgender Adolescent Stress Survey-Dysphoria (TASS-D), intended to address these gaps.MethodsThe current study recruited a U.S. national sample of TNBA (N = 444, aged 12–17; 65.5% White, 9.5% Black, 9.5% Latine, 15.5% other ethnicity; 34.7% transmasculine, 17.3% transfeminine, 38.3% nonbinary, 9.5% agender). The item pool was developed from life history calendars, a modified Delphi process, and cognitive interviews with TNBA. Scale development included factor analysis, item response theory modeling, measurement invariance testing, and reliability analyses. Associations were examined between the TASS-D and existing measures of gender dysphoria (convergent validity), gender minority stress (divergent validity), and behavioral health outcomes (criterion validity).ResultsTASS-D and its subscales (body distress and gender expression burden) were significantly and strongly associated with gender dysphoria; significantly but weakly associated with gender minority stress; and significantly associated with most indicators of psychological distress including depressive, anxiety, and posttraumatic stress symptoms, suicidal behaviors and nonsuicidal self-injury.ConclusionsThe TASS-D is a reliable and valid measure of gender dysphoria for TNBA, offering notable benefits over existing measures: It is psychometrically sound, inclusive of all gender identities, and does not assume that respondents identify binarily or desire medical transition as a terminal goal.
Depressive and anxiety symptoms among university students during the later stages of the COVID-19 pandemic in Germany - Results from the COVID 19 German Student Well-being Study (C19 GSWS)
Authors: Heumann Eileen , Helmer Stefanie M. , Busse Heide , Negash Sarah , Horn Johannes , Pischke Claudia R. , Niephaus Yasemin , Stock Christiane 
Published: September 2024

IntroductionData on the mental health of university students in Germany during the later stages of the pandemic is still limited. This study aimed to determine (1) the prevalence of anxiety and depressive symptoms among university students 1.5 years after the first COVID-19 restrictions and (2) which factors were associated with these outcomes.MethodsThe cross-sectional COVID-19 German Student Well-being Study (C19 GSWS) collected data of 6,996 students at five German universities. Associations between anxiety and depressive symptoms with sociodemographic and other factors were analyzed using multivariable logistic regression models.ResultsThe mean age of the participants was 23.9 years (SD = 4.8), 67% were female and 31% male. The prevalence for depressive symptoms was 29 and 32% for anxiety. The lack of a trusted person and financial difficulties were associated with anxiety and depressive symptoms. Being worried that someone in one’s personal network had become severely ill with COVID-19 and concern about (re)infection with COVID-19 were associated with anxiety symptoms. Those with pre-existing health conditions had an up to 1.98-times higher chance for reporting depressive symptoms (OR, 95% CI: 1.01–3.88) and an up to 2.27-times higher chance for anxiety symptoms, respectively (OR, 95% CI: 1.15–4.46).ConclusionConcepts for prevention and counseling to tackle mental health problems in students are needed and programs should take specific stressors in times of crises into account.
Comparison between standard hematological parameters and blood doping biomarkers in dried blood spots within the athlete population of Swiss Sport Integrity
Authors: Oliveira Jessica Almeida , Loria Francesco , Schobinger Céline , Kuuranne Tiia , Mumenthaler Claudia , Leuenberger Nicolas 
Published: September 2024

IntroductionThe study demonstrated the feasibility of incorporating RNA biomarkers, specifically 5-aminolevulinic acid synthase (ALAS2) and carbonic anhydrase 1 (CA1), to improve the hematological module of the Athlete Biological Passport (ABP) in routine antidoping context.ObjectiveThe aim was to investigate the implementation of reticulocyte (RET) related biomarkers, specifically ALAS2 and CA1, using quantitative reverse transcription polymerase chain reaction (RT-qPCR) on dried blood spots (DBS) from elite athletes. Hemoglobin changes over time in DBS samples was measured as well. Combining hemoglobin and messenger RNA (mRNA) analyses allowed to monitor alterations of the established marker, “DBS OFF-score”.MethodologyTen athletes were selected for sampling by the Swiss national antidoping organization, Swiss Sports Integrity (SSI). Samples were collected, transported and analyzed for ABP following the World Anti-Doping Agency (WADA) procedures and spotted onto Protein Saver DBS cards.ResultsMost athletes exhibited stable biomarker levels, except for one individual involved in ski mountaineering, who demonstrated a sustained increase in ALAS2 compared to the individual baseline. This elevation could be due to blood withdrawal or other factors, such as doping with substances outside the targeted test menu.ConclusionIn this study, RNA-biomarkers were successfully analyzed in routine blood samples, and the project demonstrated promising results for the implementation of ALAS2 and CA1 in routine analysis to complement the ABP.
Aerial Root Growth and Development Mechanism of Flowering Cherry ‘Gotenba zakura’ (Prunus incisa) and Its Relationship with Waterlogging Tolerance
Authors: Feng Xiaoxuan , Lyu Tong , Lyu Yingmin 
Published: September 2024

Flowering cherry is a renowned ornamental woody plant valued for its landscape applications and economic benefits in gardens. However, waterlogging during the rainy season in some areas causes death and heavy losses. Fortunately, we have found that the flowering cherry ‘Gotenba zakura’ (Prunus incisa Thunberg) is capable of generating aerial roots when subjected to heavy rains and prolonged floods. In this study, we conducted an associated analysis to explore the core regulating network of the aerial root growth mechanism in flowering cherry ‘Gotenba zakura’ by combining phenotypic observations, physiological assays, and transcriptome comparisons across five distinct stages. Through the analysis of the heatmap of DEGs (Differentially Expressed Genes) and the gene co-expression network (GCN), we identified genes that may play critical roles under waterlogging stress. The gene network indicates that aerial roots enhance waterlogging tolerance through ROS degradation, endogenous hormone induction, and energy production. This discovery provides a solid foundation for understanding the waterlogging tolerance of flowering cherry and offers molecular evidence for selecting promising rootstocks for breeding, aimed at improving waterlogging tolerance through grafting.
Systematic Identification and Characterization of O-Methyltransferase Gene Family Members Involved in Flavonoid Biosynthesis in Chrysanthemum indicum L.
Authors: Zhang Man , Wang Tao , Guo Qiaosheng , Su Yong , Yang Feng 
Published: September 2024

Chrysanthemum indicum L. capitulum is an enriched source of flavonoids with broad-ranging biological activities, mainly due to their anti-inflammatory, anti-cancer, immune regulation, anti-microbial activity, hepatoprotective, and neuroprotective effects. The O-methylation of various secondary metabolites has previously been demonstrated to be mainly catalyzed by S-adenosyl-L-methionine-dependent O-methyltransferase (OMT) proteins encoded by the OMT gene family. However, limited comprehensive study was published on the OMT gene family, especially the CCoAOMT subfamily, involved in the O-methylation of flavonoids in Chrysanthemum. Here, we analyzed the spatiotemporal expression patterns of C. indicum OMT genes in leaf and flower at different developmental stages. Transcriptome sequencing and qRT-PCR analysis showed that COMTs were mainly highly expressed in capitulum, especially in full bloom, while CCoAOMTs were mainly highly expressed in leaves. Correlation analysis of OMT gene expression and flavonoids accumulation revealed that four OMTs (CHR00029120, CHR00029783, CHR00077404, and CHR00078333) were putatively involved in most methylated flavonoids biosynthesis in the capitulum. Furthermore, we identified a true CCoAOMT enzyme, CiCCoAOMT1, and found that it catalyzed O-methylation of quercetin and luteolin at the 3′-OH position. In summary, this work provides an important theoretical basis for further research on the biological functions of OMTs in C. indicum.
Hand Carriage of Yeast in Student of Medicine, Nursing, and Medical Laboratory Science: Impact of Infection Control Measures
Authors: Silva Víctor , Silva Ceidy , Silva Coral , Gacitúa Rodrigo , Salas Hernán , Guzmán Neftalí , Alburquenque Claudio , Silva-Abello Viviana 
Published: September 2024

We studied yeast hand carriage of 260 healthcare students. We isolated yeasts in 27 students (10.4%), without differences between medicine, nursing, and medical laboratory science programs and gender. A significant lower prevalence of carriage was shown in the clinical cycle (2.7%) compared to the basic cycle (13.5%) (p = 0.022) and the preclinical cycle (13.5%) (p = 0.014). Increased handwashing frequency and the use of alcohol gel and antiseptic soap decreased yeast carriage. Students who applied moisturizing hand cream two or more times a day had a lower frequency of yeast carriage (3.4%) than those who did not use it or used it once a day (16.5%), showing a significant difference (p = 0.016). The most prevalent species was C. parapsilosis sensu stricto (81.5%), followed by Meyerozyma guilliermondii (C. guillermondii) (7.4%), Trichosporon mucoides (7.4%), and R. mucilagenosa (3.7%). One case showed mixed carriage of C. parapsilosis and C. albicans. All strains were sensitive to voriconazole, caspofungin, and anidulafungin. This study shows hand carriage of yeast in health students, mainly by C. parapsilosis, and the frequency of infection control measures and moisturizing hand cream is associated with colonization control.
ИЗИЛДӨӨ ИШТЕРИНЕ ӨТҮНМӨЛӨРДҮ БЕРҮҮ ЖАНА ИШТӨӨ СИСТЕМАСЫ
Authors: Арзыбаев А.М. 
Published: September 2024

В данной статье рассматривается программное обеспечение, которое ускоряет процесс подачи и обработки заявок за счѐт исключения момента физического передвижения для подачи и оценки заявок. А также система уменьшает количества ошибок при оформлении заявок путѐм выдачи и приѐма заявок по заданному шаблону.
Бул макалада өтүнмөлөрдү берүү жана баалоо үчүн физикалык кыймыл учурун жок кылуу менен өтүнмөлөрдү берүү жана иштетүү процессин тездеткен программалык камсыздоо талкууланат. Ошондой эле, система берилген калыпка ылайык тиркемелерди чыгаруу жана кабыл алуу аркылуу тиркемелерди иштетүүдө каталардын санын азайтат.
This article discusses software that speeds up the process of submitting and processing applications by eliminating the moment of physical movement for submitting and evaluating applications. And also the system reduces the number of errors when processing applications by issuing and receiving applications according to a given template.