bluuebunny/crossref_metadata_2025_split huggingface dataset:
Challenging Conventional Diagnostic Methods by Comprehensive Molecular Diagnostics: A Nationwide Prospective Comparison in Children With ALL
Authors: Boer Judith M. , Koudijs Marco J. , Kester Lennart A. , Sonneveld Edwin , Hehir-Kwa Jayne Y. , Snijder Simone , Waanders Esme , Buijs Arjan , de Haas Valérie , van der Sluis Inge M. , Pieters Rob , den Boer Monique L. , Tops Bastiaan B.J. 
Published: February 2025

PURPOSE
Treatment stratification in ALL includes diverse (cyto)genetic aberrations, requiring diverse tests to yield conclusive data. We optimized the diagnostic workflow to detect all relevant aberrations with a limited number of tests in a clinically relevant time frame.

METHODS
In 467 consecutive patients with ALL (0-20 years), we compared RNA sequencing (RNAseq), fluorescence in situ hybridization (FISH), reverse transcriptase polymerase chain reaction (RT-PCR), karyotyping, single-nucleotide polymorphism (SNP) array, and multiplex ligation-dependent probe amplification (MLPA) for technical success, concordance of results, and turnaround time.

RESULTS

To detect stratifying fusions (
ETV6
::
RUNX1
,
BCR
::
ABL1
, ABL-class,
KMT2A
r,
TCF3
::
HLF
,
IGH
::
MYC
), RNAseq and FISH were conclusive for 97% and 96% of patients, respectively, with 99% concordance. RNAseq performed well in samples with a low leukemic cell percentage or low RNA quality. RT-PCR for six specific fusions was conclusive for >99% but false-negative for six patients with alternatively fused exons. RNAseq also detected gene fusions not yet used for stratification in 14% of B-cell precursor-ALL and 33% of T-ALL. For aneuploidies and intrachromosomal amplification of chromosome 21, SNP array gave a conclusive result in 99%, thereby outperforming karyotyping, which was conclusive for 64%. To identify deletions in eight stratifying genes/regions, SNP array was conclusive in 99% and MLPA in 95% of patients, with 98% concordance. The median turnaround times were 10 days for RNAseq, 9 days for FISH, 10 days for SNP array, and <7 days for MLPA and RT-PCR in this real-world prospective study.

CONCLUSION
Combining RNAseq and SNP array outperformed current diagnostic tools to detect all stratifying genetic aberrations in ALL. The turnaround time is <15 days matching major treatment decision time points. Moreover, combining RNAseq and SNP array has the advantage of detecting new lesions for studies on prognosis and pathobiology.
Strength Training Improves Running Economy Durability and Fatigued High-Intensity Performance in Well-Trained Male Runners: A Randomized Control Trial
Authors: Zanini Michele , Folland Jonathan P. , Wu Han , Blagrove Richard C. 
Published: February 2025

ABSTRACT

Introduction
Strength training improves running economy (RE) in a non-fatigued state and performance after prolonged exercise at moderate intensity. However, it is unknown if strength training improves RE durability at marathon race intensity, or high-intensity performance akin to the final stages of a competitive race. This study quantified the effect of a supplementary 10-week strength training program on RE throughout 90 min of running in the heavy-intensity domain, and subsequent fatigued performance in runners.

Methods
Twenty-eight well-trained male runners (maximal oxygen uptake (V̇O2max) 58.6 ml·kg-1·min-1; 10 km 39:02 mm:ss) were performance-matched and randomly assigned to a habitual running-only control (E; n = 14) or supplementary strength training group (E + S; n = 14) that performed maximal strength and plyometric training twice weekly for 10 weeks. Before the training, participants performed a 90 min run at 10% Δ between lactate threshold 1 and 2 (13.1 ± 1.4 km/h, 79.7% V̇O2max). RE, quantified as oxygen cost (ml·kg-1·km-1), was recorded at 15 min intervals during the run, immediately thereafter, participants ran a time to exhaustion (TTE) at 95% pre-test V̇O2max (16.1 ± 1.6 km/h). The 90 min run and TTE were repeated after the training intervention.

Results
A large interaction effect of training x group x run time was found for RE (p = 0.003, ηp
2 = 0.13), with E + S improving vs E at 90 min (-2.1% vs +0.6; p = 0.04). For TTE, a large group x training interaction effect was detected (p = 0.004, ηp
2 = 0.28), changing by +35% in E + S and -8% in E.

Conclusions
This study demonstrated that adding strength and plyometrics training to a programme of endurance running improved RE durability and substantially increased high-intensity TTE at the end of a 90 min run in the heavy intensity domain in well-trained male runners.
Effects of Resistance and Speed on Electromyographic Activity of Thigh and Gluteal Muscles in Elite Athletes throughout Resisted Sprint Running
Authors: Macchi Robin , Hegyi András , Giroux Caroline , Rabita Giuseppe , Nordez Antoine , Hollville Enzo , Couturier Antoine , Fornasier-Santos Charly , Slawinski Jean , Morin Jean-Benoit , Lacourpaille Lilian , Guilhem Gaël 
Published: February 2025

ABSTRACT

Purpose
The application of horizontal resistance is key in sprint training to modulate velocity and promote adaptations. However, the interaction between velocity and resistance on muscle excitation remains insufficiently studied. This study assessed the electromyographic (EMG) activity of thigh and gluteal muscles in response to varying velocity and resistance during sprinting.

Methods
Thirty-seven elite athletes (27 females and 10 males) performed two trials under three conditions: a 40-meter maximal sprint without resistance and two resisted sprints applied by a robotic device with resistive forces equivalent to 25% and 75% of body mass in a sled condition. EMG activity was recorded from eight muscles in the quadriceps, hamstrings, and gluteal muscle groups for both lower limbs. Generalized mixed models were used to analyze average EMG changes with increasing velocity as a function of resistance. Statistical parametric mapping was used to assess the changes within the stance and swing phases as a function of sprint phase and resistance.

Results
Hamstring EMG activity increased with increasing velocity (+17.9% in stance phase without resistance) and decreased as resistance increased, with opposite effects observed in quadriceps muscles. Higher gluteal EMG activity was found during the late swing phase with increasing velocity and decreasing resistance. The biceps femoris long head exhibited the largest EMG increase with velocity (+23% in the stance phase) among hamstrings, while the semitendinosus showed the highest increase with reduced resistance (+27% in the stance phase).

Conclusions
External resistance and running speed influence thigh muscle activity differently, even within the same muscle group, likely due to distinct muscle architecture and function. These insights should be considered when designing sprint training programs to target specific muscle groups, avoid muscle overloading, and account for inter-individual differences to optimize performance and reduce injury risk.
Rural College Students’ Academic, Financial, and Health-Related Obstacles During the COVID-19 Pandemic: Implications for Academic Advisors
Authors: Soria Krista M. , Vakanski Stacey E. 
Published: February 2025

Abstract
We examined whether the odds of experiencing academic, financial, or health-related obstacles during the COVID-19 pandemic were significantly (p &lt; .05) different between students attending rural and suburban/urban colleges. We used data from a multi-institutional survey of 31,575 four-year college/university students attending seven rural and 62 suburban/urban colleges/universities in our analyses. Controlling for additional demographic, collegiate, and institutional variables in 19 logistic regressions, the results suggested that the odds of experiencing academic obstacles (e.g., lacking access to instructors), financial obstacles (e.g., losing wages from employment), and health-related obstacles (e.g., hospitalization from COVID-19) during the pandemic were significantly (p &lt; .001) higher for rural college students compared to suburban/urban students.
Strategi Penyandang Disabilitas dalam Mengakses Pangan dengan Keterbatasan Finansial di Kota Ambon
Authors: Sopacua Maria M 
Published: February 2025

Background: In Ambon, improving the quality of life of people with physical disabilities is still largely ignored by the local government and beyond. The gap is widening because they are stigmatized as “helpless”. This causes them to experience social discrimination and to be “othered”. As a result, it is difficult to meet their financial needs and this affects how they access food.Methods: This research uses a qualitative method conducted on the people of Ambon City from February - April 2021. Data collection techniques used observation and in-depth interviews.Results: I interviewed seven male participants based on their experiences to look at the relationship between “disabled bodies” and food miles and how the two can be intertwined. Physical geographical constraints and unfriendly constructions for people with disabilities are barriers for them to reach food destinations. On the other hand, income insecurity also makes it more difficult for them to obtain good and safe food. However, this does not mean that they have no power. To reduce the inequality of food access, they do gardening or borrow money from relatives.Conclusion: The results of this study can inform that the local government's concern is not enough to help people with physical disabilities in accessing food, but also requires support from the environment and the community.
Quantifying Point Contributions: A Lightweight Framework for Efficient and Effective Query-Driven Trajectory Simplification
Authors: Song Yumeng , Gu Yu , Li Tianyi , Li Yushuai , Jensen Christian S. , Yu Ge 
Published: February 2025

As large volumes of trajectory data accumulate, simplifying trajectories to reduce storage and querying costs is increasingly studied. Existing proposals face three main problems. First, they require numerous iterations to decide which GPS points to delete. Second, they focus only on the relationships between neighboring points (local information) while neglecting the overall structure (global information), reducing the global similarity between the simplified and original trajectories and making it difficult to maintain consistency in query results, especially for similarity-based queries. Finally, they fail to differentiate the importance of points with similar features, leading to suboptimal selection of points to retain the original trajectory information.
We propose MLSimp, a novel Mutual Learning query-driven trajectory simplification framework that integrates two distinct models: GNN-TS, based on graph neural networks, and Diff-TS, based on diffusion models. GNN-TS evaluates the importance of a point according to its globality, capturing its correlation with the entire trajectory, and its uniqueness, capturing its differences from neighboring points. It also incorporates attention mechanisms in the GNN layers, enabling simultaneous data integration from all points within the same trajectory and refining representations, thus avoiding iterative processes. Diff-TS generates amplified signals to enable the retention of the most important points at low compression rates. Experiments involving eight baselines on three databases show that MLSimp reduces the simplification time by 42%--70% and improves query accuracy over simplified trajectories by up to 34.6%.
Strength training versus high-intensity aerobic exercise: which is more effective in increasing il-10 production as an anti-inflammatory?
Authors: Wijono Wijono , Fajar Muhammad Kharis , Jatmiko Tutur , Purnomo Mochamad , Wiriawan Oce , Pramono Bayu Agung , Mustar Yetty Septiani , Kusuma I Dewa Made Aryananda Wijaya , Kurnaz Mert , Widohardhono Rachman , Nirwansyah Waristra Tyo , Pranoto Adi 
Published: February 2025

Background and Study Aim. Physical exercise is widely recognized as an effective strategy for improving health and quality of life. The purpose of this study was to analyze the effects of high-intensity and low-intensity strength training, as well as high-intensity aerobic exercise, on serum Interleukin-10 (IL-10) production.
Material and Methods. Thirty college students were recruited to undergo different training programs in each group. The study employed an experimental design with three exercise groups: high-intensity strength training (STH), low-intensity strength training (STR), and high-intensity aerobic exercise (AH). Each group included 10 participants, and serum IL-10 levels were measured before exercise and 24 hours post-exercise. To assess long-term effects, participants trained three times per week for four weeks under carefully monitored conditions.
Results. The results showed that all types of exercise significantly increased serum IL-10 levels. The AH group exhibited the highest increase, followed by the STR and STH groups. These findings align with previous studies demonstrating an increase in IL-10 following high-intensity aerobic exercise. The elevated IL-10 levels in the AH group can be attributed to improved cardiovascular capacity and the body's inflammatory response. Strength training, despite not increasing VO2max, also led to an IL-10 increase, though the effect was smaller compared to aerobic exercise.
Conclusions. High-intensity aerobic exercise is more effective at increasing IL-10 production compared to strength training. This study suggests combining both types of exercise to maximize immunological benefits. Such an approach can also enhance post-exercise recovery. It is important to consider the duration and recovery intervals, as these factors influence the immune response.
CONTRIBUIÇÃO DAS UNIVERSIDADES PARA OS OBJETIVOS DE DESENVOLVIMENTO SUSTENTÁVEL NO BRASIL E NO MUNDO
Authors: Limeira Tiago Silva , Souza Eduardo Ferreira de , Moraes Denes Martins de , Luetkmeyer Leandro , Stieven Ana Carla 
Published: February 2025

O objetivo deste trabalho foi realizar buscas e identificar as principais instituições de ensino superior reconhecidas por suas práticas de sustentabilidade e contribuição para os ODS. Foi realizado metanálise, executado 5 fases: Identificar o problema de pesquisa; Fazer a pesquisa bibliográfica; Definir os critérios de seleção e inclusão; Extração de dados; Realizar a metanálise básica. No Brasil, três universidades são destacadas na aplicação dos ODS, sendo elas: Universidade de São Paulo (USP), Universidade Federal do Rio de Janeiro (UFRJ), Universidade Federal de Santa Catarina (UFSC). Internacionalmente as principais universidades reconhecidas por suas práticas de sustentabilidade e contribuição para os ODS incluem: Universidade de Harvard (EUA), Universidade de Stanford (EUA), Universidade de Cambrige (Reino Unido), Universidade de Melbourne (Austrália). Para essas universidades, a aplicação dos ODS não é apenas uma questão de conformidade ou reputação, mas um aspecto integral de sua missão acadêmica e social, que contribui para um impacto positivo mais amplo na sociedade e no ambiente global. Os ODS são essenciais para as instituições de ensino superior porque promovem um impacto positivo abrangente, asseguram a relevância e a qualidade da educação e da pesquisa, incentivam a colaboração e a inovação, e fortalecem a reputação e a eficiência operacional das universidades. Ao integrar os ODS em suas estratégias e práticas, as instituições não só contribuem para um futuro sustentável, mas também garantem seu papel como agentes de mudança e progresso na sociedade.
ТЕРМОСТАБІЛЬНИЙ ЗАВАРНИЙ КРЕМ НА ОСНОВІ РЕЗИСТЕНТНОГО КРОХМАЛЮ З ПОДОВЖЕНИМ ТЕРМІНОМ ЗБЕРІГАННЯ
Authors: Капустян А. І. , Доценко Н. В. , Доценко С. Є. , Науменко К. І. , Гураль Л. С. , Лисюк В. М. 
Published: February 2025

Анотація. Обґрунтовано використання резистентних крохмалів(РК) та комбінації консервантів для виробництва термостабільних заварних кремів подовженого терміну зберігання. Надано характеристику нутрієнтного складу досліджуваних зразків РК. Встановлено, що вміст легкогідролізованих полісахаридів (ЛГП) у складі Зразків 1 та 2 складає близько 38–49%, Зразків 3 та 4 – 82–88%. Наявність достатньо великої кількості важкогідролізованих полісахаридів (ВГП) у складі РК Зразків 1 та 2 можна пояснити типом крохмалю (РК2, РК3), в яких ланки амілози достатньо жорстко упаковані (кристалічна структура) через генетичні особливості субстратів для отримання крохмалів, чи внаслідок ретроградних змін внаслідок модифікації. Найбільш схильними до перетравлюваності є вуглеводи Зразків 1 та 2, оскільки вони містять достатньо велику кількість ЛГП, представлених крохмалем. Перетравлюваність Зразків 3 та 4 є достатньо низькою, не дивлячись на те що вуглеводна складова представлена переважно ЛГП, що, ймовірно, можна пояснити достатньо глибокою хімічною модифікацією даних об’єктів. Для використання у рецептурі термостабільних заварних кремів рекомендовано РК «PURITY™ 87», оскільки зразки з даним видом РК характеризуються найліпшою консистенцією (при дозуванні 80 г на 500 г крему). Порівняльне оцінювання заварних кремів дозволило констатувати, що найліпшими органолептичними характеристиками володіють натуральний (класичний) заварний крем та крем на основі РК «PURITY™ 87». Заварний крем на основі РК «PURITY™87» характеризується гарною консистенцією як до, так і після випікання у складі тістових виробів, на відміну від інших дослідних зразків, отже, пропонована рецептура дозволяє отримати термостабільний заварний крем. Результати вивчення динаміки рН, титрованої кислотності та мікробіологічних критеріїв при зберіганні заварних кремів з консервантами показали, що найбільш вираженою консервувальною дією володіє суміш консервантів калію сорбату та натрію бензоату у дозуванні по 500 мг на 1 кг продукту. Згідно результатів досліджень заварний крем може зберігатися до 5 днів при температурі 12–15°С без ризику мікробіологічного псування. Саме такі температурні режими відповідають умовам зберігання випечених тістових виробів у ритейлах, кав’ярнях та інших закладах громадського харчування. Проведені дослідження дозволили обґрунтувати блок-схему технологічного процесу виробництва заварного термостабільного крему подовженого терміну зберігання на основі резистентного крохмалю, обґрунтувати критичні точки контролю та операційні програми передумови.
Rac1 Suppression by the Focal Adhesion Protein GIT2 and Podocyte Protection
Authors: Shimada Naoyuki , Matsuda Jun , Asano-Matsuda Kana , Tokuchi Maho , Aoudjit Lamine , Masztalerz Agnieszka , Lemay Serge , Takano Tomoko , Isaka Yoshitaka 
Published: February 2025

Background:
Podocytes have an intricate structure featured by numerous actin-based projections called foot processes. Rho-family of small GTPases, including Ras-related C3 botulinum toxin substrate 1 (Rac1), play important roles in actin cytoskeletal remodeling required for cell morphology and adhesion. We previously showed that Rac1 activation in podocytes causes foot process effacement and proteinuria, but the upstream and spatiotemporal regulatory mechanism directing Rac1 is largely unknown. Recently we identified the focal adhesion protein, GIT ArfGAP2 (GIT2) as one of the Rac1 interactors in human podocytes by proximity-dependent biotin identification (BioID) and proteomics.

Methods:
Systemic and podocyte-specific GIT2 knockout mice were generated and assessed for kidney phenotypes. Human podocytes with GIT2 knockdown and overexpression were established using lentiviral transduction and characterized.

Results:
GIT2 was enriched in glomeruli including podocytes in the mouse kidney. Gene deletion of Git2 in podocytes caused exacerbated proteinuria and foot process effacement when subjected to the minimal change disease model and the salt-sensitive hypertension model, which were improved by pharmacological inhibition of Rac1. In cultured podocytes, GIT2 knockdown resulted in Rac1-dependent cell spreading with marked lamellipodial protrusions, accelerated focal adhesion disassembly, and a shorter focal adhesion lifetime. In GIT2 knockdown podocytes, tyrosine phosphorylation of the focal adhesion protein, p130 Crk-associated substrate (Cas), was significantly increased, accompanied by impaired localization of the tyrosine phosphatase, PTP1B, to focal adhesions. These phenotypes observed in GIT2 knockdown podocytes were reversed by GIT2 overexpression.

Conclusions:
The results indicate that GIT2 facilitates translocation of PTP1B to focal adhesions where it dephosphorylates p130Cas, thereby suppressing local Rac1 activity and protecting against podocyte injury and proteinuria.
A (un) Assessment of Physicochemical, Microbial, and Hygienic Quality of Raw Cow Milk Produced in Dairy Herds from the Peruvian Andes
Authors: Arauco Villar Fernando , Guzmán Estremadoyro Leonor , Pantoja Esquivel Rafael , Mayorga Sánchez Noemí , Unchupaico Payano Ide , Huamán De La Cruz Alex 
Published: February 2025

The study was performed in Mantaro Valley, Junín, Perú, with the aim to evaluate the physicochemical, microbial, and hygienical quality of 40 raw cows’ milk collected from dairy herds from four provinces: Huancayo (n = 13), Concepción (n = 11), Jauja (n = 9), and Chupaca (n = 7). Physicochemical properties were quantified by evaluating the fat content, density, non-fat-solids, protein, water add, freezing point, salts, total solids, lactose, and pH using the milk analyzer Lactoscan SP. Microbial quality was determined through viable mesophilic bacteria (VMB), total coliforms (TC), fecal coliforms (FC), and yeast and mold (YMC). In addition, antibiotic presence was measured by SNAPduo*ST plus test kit and Reduction time by Methylene Blue Dye Reduction (MBRT). The results found in this work indicate that physicochemical features of raw cow milk were adequate compared to standard levels. In microbial quality, only Chupaca showed higher values (6.28 log cfu/mL) than recommended (5.3 log cfu/mL). Likewise, total bacterial/mL in Huancayo (H, 19.12 × ) and Concepcion (C, 1.18 × ) were relatively high concerning the acceptable level (1× bacteria/mL of raw milk). Antibiotic presence was found in 37.5 % (n = 15) from the total of samples (n = 40). MBRT analysis reported 32.5 %, 45.0 %, and 22.5 %, as of excellent, good, and acceptable quality, respectively. Thus, it was concluded that physicochemical properties presented an appropriate level whereas microbial quality in the areas was good but is recommendable for enriched hygienic practices, personal hygiene in milk handling due to microbial presence, and educating the public on safety issues.