bluuebunny/crossref_metadata_2025_split huggingface dataset:
Humic acid of commercial origin causes changes in gill morphology of silver catfish Rhamdia quelen exposed to acidic water
Authors: da Costa Silvio T. , Gressler Luciane T. , Sutili Fernando J. , Loebens Luíza , Fernandes Marisa N. , Lazzari Rafael , Baldisserotto Bernardo 
Published: December 2017

AbstractThe effect of dissolved organic carbon (DOC) against the burden of low pH was assessed in silver catfish Rhamdia quelen through the gills. A commercial humic acid (HA) was used as the source of DOC at 0 (control), 10, 25, and 50 mg/l. For each HA concentration, two pH levels were tested: 6.5 (control) and 5.5. After 40 days of exposure, the gills were removed and morphological variables were analyzed through light and scanning electronic microscopy. The low water pH caused a reduction in the length of filaments, number of lamellae, and gill respiratory surface area (GRSA) and an increase in chloride cells (CCs) number in the filament epithelium. When HA was added to the test water, GRSA increased and CCs proliferated in lamellae, suggesting ionic and respiratory disturbances. Scanning electronic microscopy revealed that CC morphometric variables, that is, apical area, fractional area, and density, were undisturbed by the pH reduction, but increased in the presence of HA. This study indicates that the commercial HA did not protect silver catfish against low pH stress. Instead, it caused changes that may affect vital processes such as ion regulation and ventilation and, consequently, reduce growth.
The relationship between the adult attachment and the tendency to judge others as liars
Authors: Wu Song , Huang Linjing , Li Jiahui 
Published: December 2017

AbstractThe relationship between 2 dimensions (anxiety and avoidance) of adult attachment and tendency to judge others as liars was examined in this study. We measured 308 participants' adult attachment and tendency to judge others as liars in self‐, other‐, or relationship‐oriented situations. Results supported our hypotheses by showing that (a) anxiety positively predicted the tendency to judge others as liars in self‐oriented situations and (b) avoidance moderated the relationship between anxiety and the tendency to judge others as liars in other‐ and relationship‐oriented situations. Attachment anxiety could positively predict the tendency to judge others as liars only when participants' avoidance was high. Theoretical implications and suggestions for future studies were discussed.
Lack of functional selectin-ligand interactions enhances innate immune resistance to systemic Listeria monocytogenes infection
Authors: Agbayani Gerard , Gurnani Komal , Zafer Ahmed , Sad Subash , Krishnan Lakshmi 
Published: December 2017

Abstract
Selectin-ligand interactions are important for leukocyte homing and functionality. The roles of selectin-ligand interactions in modulating immunity to intracellular infections are not completely understood. Mice lacking the expression of fucosyltransferase-IV and -VII (Fucosyltransferase-IV and -VII double knockout, FtDKO) exhibit deficient functionality of selectin-ligand interactions. We addressed the kinetics of infection and immunity to Listeria monocytogenes (LM), an intracellular pathogen, in FtDKO mice. These mice exhibited enhanced ability to clear infection and increased survival to a lethal dose of LM infection relative to wild-type (WT) C57BL/6J controls. This was associated with increased levels of neutrophils, monocytes, and dendritic cells (DCs) in the blood and/or infected organs. Adoptive transfer of bone marrow (BM) cells from FtDKO mice to WT mice resulted in enhanced neutrophil numbers and improved clearance of LM bacteria in recipients. In vivo depletion of myeloid innate immune cells, particularly neutrophils, monocytes, macrophages, and DCs, using anti-Ly-6G (RB6-8C5) monoclonal antibody, reduced the ability of FtDKO mice to curtail LM infection. Nevertheless, depletion using anti-Ly-6G (1A8) known to exclusively deplete neutrophils did not abrogate increased resistance of FtDKO mice to LM infection, suggesting a role for other myeloid innate immune cells in this model. Examination of BM hematopoietic progenitors through flow cytometry and cell culture colony-forming unit assay showed increased frequencies of granulocyte-macrophage progenitors in FtDKO relative to WT mice, Overall, our results indicate that functional selectin ligand deficiency enhances innate immune-mediated resistance to systemic LM infection despite defective leukocyte migration and lymphocyte homing.
Quantitative intravoxel incoherent motion parameters derived from whole‐tumor volume for assessing pathological complete response to neoadjuvant chemotherapy in locally advanced rectal cancer
Authors: Xu Qiaoyu , Xu Yanyan , Sun Hongliang , Chan Queenie , Shi Kaining , Song Aiping , Wang Wu 
Published: December 2017

BackgroundMany locally advanced rectal cancer (LARC) patients can benefit from neoadjuvant chemotherapy (NACT), with some achieving a pathological complete response (pCR). However, there is limited research reporting on the value of intravoxel incoherent motion (IVIM) in monitoring pCR in patients with LARC.PurposeTo identify whether IVIM parameters derived from whole‐tumor volume (WTV) before and after NACT could accurately assess pCR in patients with LARC.Study TypeProspective patient control study.PopulationFifty‐one patients with LARC before and after NACT, prior to surgery.Field Strength/SequenceIVIM‐diffusion imaging at 3T.AssessmentApparent diffusion coefficient (ADC), slow diffusion coefficient (D), fast diffusion coefficient (D*), and perfusion‐related diffusion fraction (f) values were obtained on diffusion‐weighted magnetic resonance images (DW‐MRI) using WTV methods and calculated using a biexponential model before and after NACT.Statistical TestsDWI‐derived ADC and IVIM‐derived parameters and their percentage changes (ΔADC%, ΔD%, ΔD*%, and Δf%) were compared using independent‐samples t‐test and Mann–Whitney U‐test between the pCR and non‐pCR groups. The diagnostic performance of IVIM parameters and their percentage changes were evaluated using receiver operating characteristic curves.ResultsCompared with the non‐pCR group, the pCR group exhibited significantly lower pre‐ADCmean (P = 0.003) and pre‐D values (P = 0.024), and significantly higher post‐f (P = 0.002), ΔADCmean% (P = 0.002), ΔD% (P = 0.001), and Δf% values (P = 0.017). Receiver operating characteristic curves showed that the pre‐D value had the best specificity (95.12%) and accuracy (86.27%) in predicting the pCR status, and ΔD% had the highest area under the curve (0.832) in assessing the pCR response to NACT.Data ConclusionsThe IVIM‐derived D value is a promising tool in predicting the pCR status before therapy. The percentage changes in D values after therapy may help assess the pCR status prior to surgery.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2017.
Determination of macrolide antibiotics residues in pork using molecularly imprinted dispersive solid‐phase extraction coupled with LC–MS/MS
Authors: Song Xuqin , Zhou Tong , Li Jiufeng , Su Yijuan , Xie Jingmeng , He Limin 
Published: December 2017

AbstractA class‐specific macrolide molecularly imprinted polymer was synthesized by precipitation polymerization using tulathromycin as the template and methacrylic acid as the functional monomer. The polymers revealed different specific adsorption and imprinting factor for macrolides with different spatial arrangement of side chains as well as lactonic ring size. And the molecularly imprinted polymer possessed maximum adsorption capacity (54.1 mg/g) and highest imprinting factor (2.4) toward 15‐membered ring azithromycin. On the basis of molecularly imprinted polymer dispersive solid‐phase extraction, a rapid, selective, and reproducible method for simultaneous determination of seven macrolide antibiotics residues in pork was established by using liquid chromatography with tandem mass spectrometry. At spiking levels of 5, 10, 25, and 100 μg/kg, average recoveries of seven macrolides ranged from 68.6 to 95.5% with intraday and interday relative standard deviations below 8%. The limits of detection and limits of quantification were 0.2–0.5 and 0.5–2.0 μg/kg, respectively.
Sol–gel niobia sorbent with a positively charged octadecyl ligand providing enhanced enrichment of nucleotides and organophosphorus pesticides in capillary microextraction for online HPLC analysis
Authors: Kesani Sheshanka , Malik Abdul 
Published: December 2017

AbstractA niobia‐based sol–gel organic–inorganic hybrid sorbent carrying a positively charged C18 ligand (Nb2O5‐C18(+ve)) was synthesized to achieve enhanced enrichment capability in capillary microextraction of organophosphorus compounds (which include organophosphorus pesticides and nucleotides) before their online analysis by high‐performance liquid chromatography. The sorbent was designed to simultaneously provide three different types of molecular level interactions: electrostatic, Lewis acid–base, and van der Waals interactions. To understand relative contributions of various molecular level analyte–sorbent interactions in the extraction process, two other sol–gel niobia sorbents were also created: (a) a purely inorganic sol–gel niobia sorbent (Nb2O5) and (b) an organic–inorganic hybrid sol–gel niobia sorbent carrying an electrically neutral‐bonded octadecyl ligand (Nb2O5‐C18). The extraction efficiency of the created sol–gel niobia sorbent (Nb2O5‐C18 (+ve)) was compared with that of analogously designed and synthesized titania‐based sol–gel sorbent (TiO2‐C18 (+ve)), taking into consideration that titania‐based sorbents present state‐of‐the‐art extraction media for organophosphorus compounds. In capillary microextraction with high‐performance liquid chromatography analysis, Nb2O5‐C18 (+ve) had shown 40–50% higher specific extraction values (a measure of extraction efficiency) over that of TiO2‐C18 (+ve). Compared to TiO2‐C18(+ve), Nb2O5‐C18(+ve) also provided superior analyte desorption efficiency (96 vs. 90%) during the online release of the extracted organophosphorus pesticides from the sorbent coating in the capillary microextraction capillary to the chromatographic column using reversed‐phase high‐performance liquid chromatography mobile phase.
β‐Cyclodextrin polymer@Fe3O4 based magnetic solid‐phase extraction coupled with HPLC for the determination of benzoylurea insecticides from honey, tomato, and environmental water samples
Authors: Liang Xinyu , Ma Ruiyang , Hao Lin , Wang Chun , Wu Qiuhua , Wang Zhi 
Published: December 2017

AbstractIn this work, a magnetic β‐cyclodextrin polymer was successfully prepared and used as an adsorbent for the magnetic solid‐phase extraction of six benzoylurea insecticides (diflubenzuron, triflumuron, hexaflumuron, teflubenzuron, flufenoxuron, and chlorfluazuron) from honey, tomato, and environmental water samples. The influence of the main experimental conditions on the extraction was studied. Under the optimized conditions, the β‐cyclodextrin polymer@Fe3O4 showed an excellent extraction performance for the benzoylurea insecticides. A good linearity was obtained for the analytes in the range of 3.0–800 ng/g for honey samples, 0.3–160 ng/g for tomato samples, and 0.1–80.0 ng/mL for water samples, with the correlation coefficients above 0.9998. Satisfactory repeatabilities were achieved, with the relative standard deviations less than 5.7%. The limits of detection (S/N = 3) of the method for the benzoylurea insecticides were 0.2–0.8 ng/g for honey samples, 0.04–0.10 ng/g for tomato samples, and 0.02–0.05 ng /mL for water samples. The method was successfully used for the determination of the six benzoylurea insecticides residues in honey, tomato, and environmental water samples with a satisfactory result.
Resident Accuracy of Musculoskeletal Palpation With Ultrasound Verification
Authors: Mehta Priyesh , Rand Ethan B. , Visco Christopher J. , Wyss James 
Published: December 2017

ObjectivesThe purpose of this observational study was to determine the accuracy of musculoskeletal palpation of the medial joint line of the knee, medial patellar tendon, and posterior tibialis tendon verified by ultrasound imaging among physical medicine and rehabilitation residents.MethodsEighteen physical medicine and rehabilitation resident physicians at a single specialized institution were asked to identify the medial joint line of the knee, medial patellar tendon, and posterior tibialis tendon on 2 separate standardized patient models during a single data collection. They were asked to place a paper clip flat on the surface of the skin parallel to the specified anatomic structure. A high‐frequency linear array transducer was used to identify whether the paper clip was correctly placed over the structures and to measure the distance from the intended structure.ResultsThe accuracy rates for palpation of the medial joint line, medial patellar tendon, and posterior tibialis tendon in both models were 14%, 36%, and 28%, respectively, for all levels of residents. Accuracy rates for all of the structures by level of education were 19%, 29%, and 31% for postgraduate years 2, 3, and 4. Median confidence scores were 3.75, 3.5, and 2 for the medial joint line, medial patellar tendon, and posterior tibialis tendon.ConclusionsThis study highlights the level of inaccuracy of musculoskeletal palpation skills and draws further attention to an area of much‐needed improvement in our musculoskeletal residency training programs. Ultrasound imaging is an effective noninvasive method for providing swift feedback to medical students and residents and thereby reduce the instances of inaccurate musculoskeletal palpation.
The effects of concurrent chemoradiation therapy to the base of tongue in a preclinical model
Authors: Benedict Peter A. , Ruiz Ryan , Verma Avanti , Dion Gregory R. , Oh Philmo , Wang Binhuan , Ahmed Omar H. , Hiwatashi Nao , Bing Renjie , Victor Kristen , Hu Kenneth S. , Johnson Aaron , Branski Ryan C. , Amin Milan R. 
Published: December 2017

Objectives/HypothesisTo develop a clinically relevant model of oropharyngeal concurrent chemoradiation therapy (CCRT) in order to quantify the effects of CCRT on tongue function and structure. CCRT for advanced oropharyngeal cancer commonly leads to tongue base dysfunction and dysphagia. However, no preclinical models currently exist to study the pathophysiology of CCRT‐related morbidity, thereby inhibiting the development of targeted therapeutics.Study DesignAnimal model.MethodsTwenty‐one male Sprague‐Dawley rats were randomized into three groups: 2 week (2W), 5 month (5M), and control (C). The 2W and 5M animals received cisplatin, 5‐fluorouracil, and five fractions of 7 Gy to the tongue base; the C animals received no intervention. In vivo tongue strength and displacement, as well as hyoglossus muscle collagen content, were assessed. Analyses were conducted 2 weeks or 5 months following completion of CCRT in the 2W and 5M groups, respectively.ResultsPeak tetanic and twitch tongue forces were significantly reduced in both 2W and 5M animals compared to controls (tetanic: P = .0041, P = .0089, respectively; twitch: P = .0201, P = .0020, respectively). Twitch half‐decay time was prolonged in 2W animals compared to controls (P = .0247). Tongue displacement was significantly reduced across all testing parameters in 5M animals compared to both the C and 2W groups. No differences in collagen content were observed between experimental groups.ConclusionsThe current study is the first to describe a preclinical model of CCRT to the head and neck with an emphasis on clinical relevance. Tongue strength decreased at 2 weeks and 5 months post‐CCRT. Tongue displacement increased only at 5 months post‐CCRT. Fibrosis was not detected, implicating alternative causative factors for these findings.Level of EvidenceNA Laryngoscope, 1783–1790, 2018
Intranasal tissue necrosis associated with opioid abuse: Case report and systematic review
Authors: Morrison Danielle A. , Wise Sarah K. , DelGaudio John M. , Chowdhury Naweed I. , Levy Joshua M. 
Published: December 2017

ObjectiveOpioid abuse is a common disorder affecting over 2 million Americans. Intranasal tissue necrosis is a previously described sequela of nasal opioid inhalation, with a similar presentation to invasive fungal rhinosinusitis (IFRS). The goal of this case report and systematic review is to evaluate the evidence supporting this uncommon disease, with qualitative analysis of the presentation, management and treatment outcomes.Data SourcesMEDLINE, EMBASE, Google Scholar, Scopus, and Web of Science.Review MethodsPreferred Reporting Items for Systematic Reviews and Meta‐analyses guidelines were utilized to identify English‐language studies reporting intranasal mucosal injury associated with prescription opioid abuse. Primary outcomes included clinical presentation, treatment strategies, and outcomes.ResultsSystematic review identified 61 patients for qualitative analysis. Common clinical features include facial pain without a history of chronic sinusitis or known immunodeficiency. Diagnostic nasal endoscopy revealed superficial debris with underlying tissue necrosis, consistent with a preliminary diagnosis of IFRS. Characteristic pathologic findings include mucosal ulceration with an overlying acellular substrate, often with polarizable material. Fungal colonization is often reported, with several accounts of angiocentric invasion in immunocompetent patients. Complete symptom resolution is expected following surgical debridement with cessation of intranasal opioid inhalation, with 89% of identified patients experiencing a complete resolution of disease.ConclusionIntranasal opioid abuse is a prevalent condition associated with chronic pain and tissue necrosis that is clinically concerning for invasive fungal disease. Whereas IFRS must be excluded, even in patients without known immunodeficiency, complete resolution of symptoms can be expected following surgical debridement with cessation of opioid abuse. Laryngoscope, 1767–1771, 2018
Detailed Analysis of Pancreatic Tumor Cell Attachment on Gradient PDEGMA Brushes
Authors: Großhans Sabine , Lilge Inga , Schönherr Holger 
Published: December 2017

AbstractPoly(di(ethylene glycol) methyl ether methacrylate) (PDEGMA) brushes show interesting thermoresponsive behavior that can be applied for cell release surfaces. Here it is shown that PDEGMA thickness gradients, which are synthesized by surface‐initiated atom transfer radical polymerization, allow the systematic and precise analysis of the attachment of PaTu 8988 cells. By pumping the polymerization solution into the reactor with vertically fixed initiator samples, PDEGMA gradients with linearly increasing dry ellipsometric thickness with typical slopes of 2.5 nm cm−1 are obtained. A very narrow transition of PaTu 8988t cell attachment is observed that starts for a thickness larger than 7.1 ± 0.2 nm. For PDEGMA layers thicker than 8.7 ± 0.2 nm no attached cells are found. This very narrow transition in brush properties within a thickness difference of <2 nm from cell‐adherent to cell‐nonadherent can be determined in much greater detail than before owing to the thickness gradients with shallow slope.
Autoacceleration in Bulk Free‐Radical Polymerization: Effect of Chain Transfer
Authors: Victoria‐Valenzuela David , Herrera‐Ordonez Jorge , Ricardo Cristy L. Azanza , Estévez Miriam 
Published: December 2017

AbstractThe model and methodology for estimating diffusion‐controlled rate coefficients for the methyl methacrylate polymerization system reported in Parts I and II of this series is extended to include the chain transfer reaction to an agent (CTA). Theoretical and experimental results exhibit minima in the rate of polymerization curves at low conversions as it is observed in the absence of CTA. Such minima are proposed to be the onset of the autoacceleration effect where the termination reaction undergoes a transition from chemically controlled to diffusion controlled where the segmental mobility of the chain ends plays a key role. As the CTA concentration increases, the conversion at which the minimum is presented is higher and the autoacceleration is less pronounced. This behavior is explained in terms of the effect of polymer chain entanglements and segmental mobility of the chain ends on the termination rate coefficient k
t.
A recursion theoretic characterization of the Topological Vaught Conjecture in the Zermelo‐Fraenkel set theory
Authors: Gregoriades Vassilios 
Published: December 2017

AbstractWe prove a recursion theoretic characterization of the Topological Vaught Conjecture in the Zermelo‐Fraenkel set theory by using tools from effective descriptive set theory and by revisiting the result of Miller that orbits in Polish G‐spaces are Borel sets.
Tunable Drug Loading and Reinforcement of Polycaprolactone Films by Means of Electrospun Nanofibers of Glycolide Segmented Copolymers
Authors: Márquez Yolanda , Franco Lourdes , Turon Pau , del Valle Luís J. , Puiggalí Jordi 
Published: December 2017

AbstractElectrospinning of a segmented copolymer having polyglycolide hard segments is successfully performed from 1,1,1,3,3,3‐hexafluoroisopropanol solutions. During the process, a bactericidal agent, i.e., chlorhexidine (CHX), is effectively loaded, which results in nanofibers with a smaller diameter because of the change in solution conductivity. New fabrics based on molding of alternate layers of poly(ε‐caprolactone) (PCL) films and the electrospun scaffolds of the segmented copolymer are prepared and characterized. The thermal molding process renders a PCL matrix homogeneously reinforced with nanofibers that compensate for the loss of mechanical properties caused by incorporation of CHX. Release of CHX is evaluated in different media. Results vary depending on the layer where the drug is incorporated. Thus, systems with an immediate bacteriostatic effect, as well as systems with a potential long term antimicrobial effect, are obtained. Growth inhibition and adhesion assays demonstrate the fast bactericidal effect of samples with CHX loaded in its outer layers.
Structural Evolution of PCL during Melt Extrusion 3D Printing
Authors: Liu Fengyuan , Vyas Cian , Poologasundarampillai Gowsihan , Pape Ian , Hinduja Sri , Mirihanage Wajira , Bartolo Paulo 
Published: December 2017

AbstractScrew‐assisted material extrusion technique is developed for tissue engineering applications to produce scaffolds with well‐defined multiscale microstructural features and tailorable mechanical properties. In this study, in situ time‐resolved synchrotron diffraction is employed to probe extrusion‐based 3D printing of polycaprolactone (PCL) filaments. Time‐resolved X‐ray diffraction measurements reveals the progress of overall crystalline structural evolution of PCL during 3D printing. Particularly, in situ experimental observations provide strong evidence for the development of strong directionality of PCL crystals during the extrusion driven process. Results also show the evidence for the realization of anisotropic structural features through the melt extrusion‐based 3D printing, which is a key development toward mimicking the anisotropic properties and hierarchical structures of biological materials in nature, such as human tissues.