bluuebunny/crossref_metadata_2025_split huggingface dataset:
PEMANFAATAN SITUS PURBAKALA PETIRTAAN NGAWONGGO SEBAGAI SUMBER BELAJAR SEJARAH BAGI PESERTA DIDIK SMP HASYIM ASY’ARI KECAMATAN TAJINAN MALANG
Authors: Hasanah Siti Muawanatul , Safida Aulia 
Published: October 2024

Situs Purbakala Petirtaan Ngawonggo merupakan Situs Arkeologi berupa kolam petirtaan pada zaman kerajaan abad 10 Masehi yang ditemukan di Dusun Nanasan Desa Ngawonggo Kec. Tajinan Kab. Malang yang identik dengan kebudayaan jawa kental dengan suasananya yang masih sangat asri dan nuansa pedesaan inilah yang menjadi dambaan pengunjung asal kota saat berkunjung ke destinasi wisata. Situs purbakala petirtaan ngawonggo identik dengan tomboannya. Tomboan dalam bahasa Jawa berarti "obat-obatan". Dalam konteks ini adalah sekelompok mahasiswa KKN-T UNIRA MALANG mengadakan studytour bersama peserta didik SMP Hasyim Asy’ari bertema pengenalan Situs Purbakala petirtaan Ngawonggo. Tujuannya untuk mengetahui relevansi koleksi Situs Purbakala petirtaan Ngawonggo dalam materi pembelajaran sejarah, dan implementasi pemanfaatan situs purbakala petirtaan Ngawonggo dalam pembelajaran sejarah kebudayaan.
PENGGUNAAN FISHFINDER UNTUK PENENTUAN LOKASI RUMPON DAN FISHING GROUND PADA MASYARAKAT NELAYAN TRADISIONAL LHOK KRUENG ACEH
Authors: Muhammad Muhammad , Setiawan Ichsan , Rizwan Thaib , Haridhi Haekal Azief , Yuni Syarifah Meurah , Arif Salmawaty 
Published: October 2024

Tujuan kegiatan pengabdian ini adalah untuk membantu nelayan-nelayan tradisional Lhok Krueng Aceh, Banda Aceh agar dapat memahami dan mempraktikkan cara menggunakan fishfinder untuk penentuan rumpon dan fishing ground. Masalah yang dihadapi nelayan tradisional pada setiap kegiatan melaut adalah tidak terbiasa dalam menggunakan fishfinder. Oleh karena itu, solusi yang ditawarkan adalah melaksanakan pelatihan pengoperasian fishfinder untuk penentuan lokasi rumpon dan fishing ground pada nelayan tradisional dan memberikan pelatihan untuk membaca dan memanfaatkan peta. Hasil pengabdian ini, tingkat keberhasilan kemampuan nelayan dalam penggunaan fishfinder dan membaca peta mencapai 75% diukur dengan cara membandingkan kemampuan nelayan sebelum pelatihan yang hanya 10%. Dengan demikian, permasalahan yang dialami oleh nelayan dapat diatasi dengan metode ajar dan praktik lapangan.
STRATEGI PENGELOLAAN DANA DESA DALAM MENINGKATKAN KEPEDULIAN SOSIAL PADA MASA PANDEMI COVID-19 DI DESA PETIYINTUNGGAL DUKUN GRESIK
Authors: Irawan Agus , Wahyudi Imam , Ubaidillah Ahmad , Aryanto Sherif Juniar 
Published: October 2024

Indonesia dilanda pandemi Covid-19 sejak tanggal 2 Maret 2020, hingga saat ini pemerintah dan masyarakat telah melewati beberapa gelombang pandemi Covid-19 mulai dari gelombang varian Alpha di 2020, Delta di 2021 dan penyebaran Varian Omicron pada Januari 2022. Untuk itu, Pemerintah Desa Petiyintunggal Dukun Gresik ini melakukan analisa dan kajian prioritas Dana Desa, serta merancang prosedur yang baik dengan tujuan supaya dapat meningkatkan kepedulian sosial pada warga masyarakat dimasa pandemi Covid-19, sehingga fokus penelitian ini pada: (1) bagaimana mitigasi dan penangan bencana non alam Desa Aman COVID-19 di Desa Petiyintunggal Dukun Gresik? (2) bagaimana strategi pendistribusian dana desa dalam meningkatkan kepedulian sosial pada Covid-19 di Desa Petiyintunggal Dukun Gresik? Penelitian ini menggunakan metode kualitatif dengan pendekatan deskriptif analitis. Hasil penelitian ini menunjukkan bahwa (1) dalam melakukan kegiatan mitigasi dan penanganan bencana non alam Desa Aman COVID, Penggunaan Dana Desa untuk mendukung aksi Desa aman COVID-19 antara lain: a) membentuk Pos Jaga Desa; b) sosialisasi dan edukasi adaptasi kebiasaan baru serta penerapan secara ketat protocol kesehatan; c) menyiapkan tempat cuci tangan/hand sanitizer; pembelian masker, vitamin dan obat sesuai arahan Satgas COVID-19 serta kebutuhan lainnya, termasuk mendukung operasional tugas Relawan Desa Aman COVID-19. (2) Strategi pendistribusian dana desanya dilakukan secara langsung dan tidak langsung melalui Relawan Desa COVID-19 yang besaran BLT-DD ditetapkan sebesar Rp. 300.000,00 dibayarkan setiap bulan selama 12 bulan dengan jumlah calon penerima manfaat 104 KK; yang tidak memenuhi syarat 24 KK dan yang memenuhi syarat 80 KK. Adapun metode/cara pendistribusiannya; Diantar ke rumah, Diambil di kantor desa, dan Via Transfer Bank (non tunai).
PENGENALAN METODE MENGAJI AL-QUR’AN DENGAN MENGGUNAKAN METODE UMMI PADA SANTRIWATI SANTIWIT SONGKHLA TECHNOLOGICAL COLLEGE
Authors: Achadah Alif 
Published: October 2024

Tri Dhrama Perguruan tinggi merupakaan bentuk kewajiban yang ada di dalam perguruan tinggi dan terdiri dari tiga point utama yaitu pendidikan, pengabdian dan juga penelitian. Salah satu wujud implementasi atau bentuk nyata dari pengalaman tri dharma perguruan tinggi yaitu dengan mengikuti kegiatan KKN atau kegiatan Kuliah Kerja Nyata. Kuliah Kerja Nyata merupakan kegiatan yang dilaksanakan oleh mahasiswa dengan terjun secara langsung ke masyarakat. Dalam kegiatan KKN PPL Thailand sendiri banyak kegiatan yang diikuti oleh mahasiswa. Salah satu contoh kegiatan yang dilakukan yaitu dengan mengadakan kegiatan pembelajaran al-quran dengan menggunakan metode ummi yang memiliki motto motto pendidikan yaitu mudah, menyenangkan dan menyentuh hati. Tahapan pembelajaran Alquran dengan metode ummi terdiri dari pembukaan, appersepsi, penanaman konsep, pemahaman, keterampilan, evaluasi, dan penutup. metode baru dapat membantu para santriwati dalam mempelajari serta memaknai al-quran itu sendiri, selain mengenalkan metode pembelajaran ini kepada para santriwati dan juga pengajar yang ada.

Kata Kunci: Kuliah Kerja Nyata, Mengaji, Metode Ummi, Pengabdian Masyarakat
A differentiation protocol for generating pancreatic delta cells from human pluripotent stem cells
Authors: Zhang Tongran , Wang Nannan , Liao Zhiying , Chen Jingyi , Meng Hao , Lin Haopeng , Xu Tao , Chen Lihua , Zhu Ling-Qiang , Liu Huisheng 
Published: October 2024

In this protocol, we detail a seven-stage differentiation methodology for generating pancreatic delta cells (SC-delta cells) from human pluripotent stem cells (hPSCs). In the first step, definitive endoderm is generated by activin A and CHIR99021, followed by induction of primitive gut tube and posterior foregut by treatment with FGF7, SANT1, LDN193189, PdBU, and retinoic acid (RA). The subsequent endocrine generation and directed SC-delta cell induction is achieved by a combined treatment of the FGF7 with FGF2 during stage 4 and 5, together with RA, XXI, ALK5 inhibitor II, SANT1, Betacellulin and LDN193189. The planar cultivation is converted to a suspended system after stage 5, allowing cells to aggregate into delta cell-containing spheroids. The differentiation takes approximately 4-5 weeks for delta cell generation and an additional 1-2 weeks for cell expansion and evaluation. We believe that this amenable and simplified protocol can provide a stable source of SC-delta cells from efficient differentiation, facilitating further investigation of the physiological role of delta cells as well as refinement of islet cell therapeutic strategies.
PD-1/PD-L1 and coronary heart disease: a mendelian randomization study
Authors: Zeng Liangjia , Liang Yinglan , Zhou Ruoyun , Yang Wenting , Chen Kexin , He Baixin , Qiu Yuqing , Liu Linglong , Zhou Deyang , Xiao Zhaolin , Liang Haowen , Zhang Binghua , Li Renyu , Yu Lihong , Yi Min , Lin Xiaozhen 
Published: October 2024

IntroductionIt has been found that programmed cell death protein-1 (PD-1) or its ligand PD-L1 may play an important role in the onset and progression of coronary heart disease (CHD). Thus, we conducted this mendelian randomization analysis (MR) to estimate the causal relationship between PD-1/PD-L1 and 5 specific CHDs (chronic ischemic heart disease, acute myocardial infarction, angina pectoris, coronary atherosclerosis, and unstable angina pectoris), complemented by gene set enrichment analysis (GSEA) for further validation.MethodsPublicly available summary-level data were attained from the UK Biobank with genetic instruments obtained from the largest available, nonoverlapping genome-wide association studies (GWAS). Our analysis involved various approaches including inverse variance-weighted meta-analysis, alternative techniques like weighted median, MR-Egger, MR-multipotency residuals and outliers detection (PRESSO), along with multiple sensitivity assessments such as MR-Egger intercept test, Cochran's Q test, and leave-one-out sensitivity analysis to evaluate and exclude any anomalies.ResultsGene expression profile (GSE71226) was obtained from Gene Expression Omnibus (GEO) database for GSEA. IVW analysis showed a causal association between PD-1 and chronic ischemic heart disease (OR, 0.997; 95%CI, 0.995-0.999; P, 0.009), chronic ischemic heart disease and PD-1 (beta, −3.1; 95%CI, −6.017 to −0.183; P, 0.037), chronic ischemic heart disease and PD-L1 (beta, −3.269; 95%CI, −6.197 to −0.341; P, 0.029). No significant causal relationship was found between PD-1/PD-L1 and other 4 CHDs. The accuracy and robustness of these findings were confirmed by sensitivity tests. GSEA found that the KEGG pathway and related core genes of “PD-L1 expression and PD-1 checkpoint pathway in cancer” pathway were downregulated in CHD.DiscussionThis study provided evidence of a bidirectional causal relationship between PD-1 and chronic ischemic heart disease and a protective association between chronic ischemic heart disease and PD-L1.
Habitat quality assessment on the Qinghai-Tibet plateau across vegetation ecoregions using InVEST and Geodetector models
Authors: Jiang Farui , Liu Chonghao , Zhao Jianan , Jiang Baode , Fan Fengyan 
Published: October 2024

IntroductionThe Qinghai-Tibet Plateau holds a significant position in the global ecosystem, with its unique high altitude and complex geographical features fostering rich biodiversity. The plateau’s high-altitude environment and distinctive climate system significantly influence the Asian monsoon and regional hydrological cycles. Its vast glaciers and snow reserves are crucial in regulating the Asian climate. While previous studies have analyzed the habitat quality of the Qinghai-Tibet Plateau, significant variations exist across different vegetation ecoregions, warranting further investigation into these variations and their influencing factors.MethodsThis study conducted a comprehensive assessment of habitat quality on the Qinghai-Tibet Plateau by analyzing the impacts of human activities, climate change, and grazing intensity. Using the InVEST Model and Geodetector Model, we evaluated habitat quality across the plateau’s six vegetation ecoregions from 2000 to 2020. The efficacy of ecological red-line policies in actual conservation efforts was also examined.ResultsThe results indicate that habitat quality among vegetation ecoregions is shaped by a complex interaction of geographical and human-induced factors, leading to notable spatiotemporal variations. From 2000 to 2020, the quality of habitats was significantly impacted by human activities, climate change, grazing intensity, and land-use changes. These effects were especially prominent during the period from 2005 to 2010.DiscussionThe changes in habitat quality on the Qinghai-Tibet Plateau are influenced by multiple driving factors, with significant differences in the drivers across various vegetation ecoregions. The ecological redline policy has played an important role in protecting the ecological environment in key areas, particularly in regions with high human intervention, where its effectiveness is more pronounced. In future ecological management, it is essential to strengthen conservation measures based on regional specificity. By comprehensively considering the impact of human activities and natural factors, developing more targeted management strategies is crucial for improving habitat quality.
FMT intervention decreases urine 5-HIAA levels: a randomized double-blind controlled study
Authors: Wang Lihong , Yu Lianhu , Liu Zhiyue , Che Chao , Wang Yu , Zhao Yongheng , Zhu Mengna , Yang Guang , Cao Aihua 
Published: October 2024

BackgroundAutism spectrum disorder (ASD) is often linked to gastrointestinal issues and altered serotonin metabolism. Emerging evidence suggests gut microbiota influence both, with fecal microbiota transplantation (FMT) offering a potential therapeutic approach. However, its impact on serotonin metabolism and ASD symptoms is not well understood. In this study, we aimed to evaluate the clinical effects of FMT and examine changes in specific urinary metabolites in children with ASD.MethodsA randomized double-blind controlled trial was performed to evaluate the clinical effects of FMT on GI and ASD-related symptoms. Gastrointestinal symptoms were assessed using the Gastrointestinal Symptom Rating Scale (GSRS), and the ASD-related symptoms were assessed using the Childhood Autism Rating Scale (CARS), Aberrant Behavior Checklist (ABC), and Social Responsiveness Scale (SRS) scores. Urinary metabolites were analyzed by homogeneous enzyme immunoassay using commercially available kits.ResultsSignificant improvements in GI and core ASD symptoms were observed following FMT intervention. The average GSRS scores decreased from 30.17 (before) to 19 (after; p &lt; 0.0001), CARS scores decreased from 36.22 to 33.33 (p &lt; 0.0001), SRS scores decreased from 151.17 to 137.5 (p = 0.0002), and the ABC scores decreased 76.39 to 53.17 (p &lt; 0.0001) in the FMT group. However, in the placebo group, GSRS, CARS, and SRS scores showed no significant changes, while ABC scores decreased from 72 to 58.75 (p = 0.034). The FMT group also showed a significant reduction in urinary 5-hydroxyindoleacetic acid (5-HIAA) levels from 8.6 to 7.32 mg/L (p = 0.022), while other metabolites showed no significant changes.ConclusionFMT is a safe and effective treatment for improving GI and core symptoms in children with ASD, with 5-HIAA showing potential as a urinary biomarker for treatment response.
Exploring the association between phytopharmaceutical use and antibiotic prescriptions in upper respiratory infections: results from a German cohort study evaluating the impact of naturopathy qualifications of general practitioners using routine data
Authors: Wetzel Anna-Jasmin , Laux Gunter , Joos Stefanie , Musselmann Berthold , Valentini Jan 
Published: October 2024

BackgroundAntibiotic resistance is a significant global health threat, exacerbated by inappropriate prescribing practices, particularly for upper respiratory infections that are predominantly viral. Complementary and Integrative Medicine (CIM), including the use of phytopharmaceuticals, offers a potential strategy to reduce antibiotic prescriptions.ObjectiveThis study aimed to describe the impact of General Practitioners’ (GPs) naturopathy (NP) qualifications and phytopharmaceutical prescriptions on the rate of antibiotic prescribing for upper respiratory infections (RTI).MethodsWe conducted a retrospective cohort study using routine data from the CONTinuous morbidity registration Epidemiologic NeTwork (CONTENT), which includes over 200,000 patients across four federal states in Germany. The study included data from n = 36 GPs who recorded at least one ICD-10 diagnosis of RTI. Antibiotic and phytopharmaceutical prescriptions were identified and analyzed through mixed-effects logistic regression models to explore the influence of GPs’ naturopathy qualifications and phytopharmaceutical use on antibiotic prescribing patterns.ResultsThe study included 40,344 patients managed by 36 GPs. Prescriptions of phytopharmaceuticals significantly reduced the likelihood of antibiotic use (OR 0.48, 95% CI 0.45–0.52). Additionally, holding a naturopathy qualification was associated with lower rates of antibiotic prescriptions (OR 0.73, 95% CI 0.69–0.78). The interaction between naturopathy qualification and phytopharmaceutical prescriptions also showed a significant effect (OR 1.43, 95% CI 1.27–1.62). Patient’s year of birth influenced prescribing patterns indicating a reduction of antibiotic prescriptions for younger patients, while patients’ gender did not reveal a significant effect.ConclusionPrescriptions of phytopharmaceuticals were significantly associated with a decrease antibiotic prescriptions among GPs, especially when combined with naturopathy qualifications. Training in naturopathic approaches could enhance antibiotic stewardship efforts in primary care settings, suggesting that broader integration of CIM elements into medical training could be beneficial in mitigating antibiotic resistance.
Case report: Novel deletions in the 6p21.33 involving the CSNK2B gene in patients with Poirier-Bienvenu neurodevelopmental syndrome and literature review
Authors: Zhang Xuan , Lu Hongjuan , Ji Yichen , Sun Wei 
Published: October 2024

BackgroundSeizures have been identified in most patients with CSNK2B-related Poirer-Bienvenu Neurodevelopment syndrome (POBINDS). Detailed descriptions of seizure phenotypes, various genotypes, and long-term follow-up visits are required for clinicians to provide reasonable clinical management for such patients.Case summaryWe report two new Chinese patients with varying sizes of 6p21.33 deletions encompassing the CSNK2B gene who presented with intellectual disability and seizures. Furthermore, we conducted a literature review of previously reported patients with 6p21.33 deletions or CSNK2B variants. We summarized and analyzed the clinical characteristics of these patients with seizures. The occurrence of a biphasic pattern of epilepsy and pharmacoresistant epilepsy in patients with CSNK2B variants is severely underestimated. One of our patients underwent a long follow-up period and presented with comprehensive disease progression.ConclusionOur data suggest that the CSNK2B variant or 6p21.33 deletion should be considered in patients with intellectual disability and epilepsy, especially those characterized by biphasic patterns and digital anomalies.
Heterogeneity between subgroups of first-line chemoimmunotherapy for extensive-stage small cell lung cancer patients: a meta-analysis and systematic review
Authors: Kang Wenwen , Cheng Jing , Pan Luyun , Zhan Ping , Liu Hongbing , Lv Tangfeng , Han Hedong , Song Yong 
Published: October 2024

ObjectivesDifferences in clinicopathological characteristics of extensive-stage small cell lung cancer (ES-SCLC) patients may influence the immune response. This study aims to evaluate the heterogeneity of response to first-line chemoimmunotherapy between subgroups in ES-SCLC to screen out suitable populations.Materials and methodsWe searched the PubMed, EMBASE, and Cochrane Library databases from inception to December 3, 2022 for randomized controlled trials (RCTs) of ES-SCLC chemoimmunotherapy. We also reviewed main conferences from January 1, 2021 to October 1, 2023. A trial-specific hazard ratio (HR) ratio for each subgroup was calculated, and these ratios were then pooled using the deft approach.ResultsA total of 9 RCTs with 4099 patients were finally included. The pooled ratios were 0.92 (95% CI = 0.77 to 1.09) for OS-HRs and 0.79 (95% CI = 0.55 to 1.13) for PFS-HRs in women versus men. The pooled ratios of OS-HRs and PFS-HRs in patients with positive versus negative PD-L1 expression were 1.26 (95% CI = 0.91 to 1.73) and 1.08 (95% CI = 0.77 to 1.52), respectively. The pooled ratios of OS-HRs and PFS-HRs in patients without versus with brain metastasis were 0.77 (95% CI = 0.59 to 1.01) and 0.71 (95% CI = 0.44 to 1.12). No statistically significant differences were also found in terms of subgroups for age, liver metastasis, smoking status, ECOG PS, LDH level, type of platinum salt and race.ConclusionWomen or patients with negative PD-L1 expression or with LDH ≤ ULN or without brain metastasis tend to benefit more from first-line chemoimmunotherapy in ES-SCLC. More trials are needed to prospectively validate the therapeutic heterogeneity among clinicopathological characteristics.Systematic review registrationhttps://inplasy.com/inplasy-2023-3-0064/ identifier, INPLASY202330064.
Identification and validation of matrix metalloproteinase hub genes as potential biomarkers for Skin Cutaneous Melanoma
Authors: Zhang Zhongyi , Zhao Mei , Zhou Zubing , Ren Xiaodan , He Yunliang , Shen Tao , Zeng Hongping , Li Kai , Zhang Yong 
Published: October 2024

ObjectivesThe role of matrix metalloproteinases (MMPs) in Skin Cutaneous Melanoma (SKCM) development and progression is unclear so far. This comprehensive study delved into the intricate role of MMPs in SKCM development and progression.MethodsRT-qPCR, bisulfite sequencing, and WES analyzed MMP gene expression, promoter methylation, and mutations in SKCM cell lines. TCGA datasets validated findings. DrugBank and molecular docking identified potential regulatory drugs, and cell line experiments confirmed the role of key MMP genes in tumorigenesis.ResultsOur findings unveiled significant up-regulation of MMP9, MMP12, MMP14, and MMP16, coupled with hypomethylation of their promoters in SKCM cell lines, implicating their involvement in disease progression. Mutational analysis highlighted a low frequency of mutations in these genes, indicating less involvement of mutations in the expression regulatory mechanisms. Prognostic assessments showcased a significant correlation between elevated expression of these genes and poor overall survival (OS) in SKCM patients. Additionally, functional experiments involving gene silencing revealed a potential impact on cellular proliferation, further emphasizing the significance of MMP9, MMP12, MMP14, and MMP16 in SKCM pathobiology.ConclusionThis study identifies Estradiol and Calcitriol as potential drugs for modulating MMP expression in SKCM, highlighting MMP9, MMP12, MMP14, and MMP16 as key diagnostic and prognostic biomarkers.