bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abrogated Freud-1/Cc2d1a Repression of 5-HT1A Autoreceptors Induces Fluoxetine-Resistant Anxiety/Depression-Like Behavior
Authors: Vahid-Ansari Faranak , Daigle Mireille , Manzini M. Chiara , Tanaka Kenji F. , Hen René , Geddes Sean D. , Béïque Jean-Claude , James Jonathan , Merali Zul , Albert Paul R. 
Published: December 2017

Freud-1/Cc2d1a represses the gene transcription of serotonin-1A (5-HT1A) autoreceptors, which negatively regulate 5-HT tone. To test the role of Freud-1in vivo, we generated mice with adulthood conditional knock-out of Freud-1 in 5-HT neurons (cF1ko). IncF1komice, 5-HT1A autoreceptor protein, binding and hypothermia response were increased, with reduced 5-HT content and neuronal activity in the dorsal raphe. ThecF1komice displayed increased anxiety- and depression-like behavior that was resistant to chronic antidepressant (fluoxetine) treatment. Using conditional Freud-1/5-HT1A double knock-out (cF1/1A dko) to disrupt both Freud-1 and 5-HT1A genes in 5-HT neurons, no increase in anxiety- or depression-like behavior was seen upon knock-out of Freud-1 on the 5-HT1A autoreceptor-negative background; rather, a reduction in depression-like behavior emerged. These studies implicate transcriptional dysregulation of 5-HT1A autoreceptors by the repressor Freud-1 in anxiety and depression and provide a clinically relevant genetic model of antidepressant resistance. Targeting specific transcription factors, such as Freud-1, to restore transcriptional balance may augment response to antidepressant treatment.SIGNIFICANCE STATEMENTAltered regulation of the 5-HT1A autoreceptor has been implicated in human anxiety, major depression, suicide, and resistance to antidepressants. This study uniquely identifies a single transcription factor, Freud-1, as crucial for 5-HT1A autoreceptor expressionin vivo. Disruption of Freud-1 in serotonin neurons in mice links upregulation of 5-HT1A autoreceptors to anxiety/depression-like behavior and provides a new model of antidepressant resistance. Treatment strategies to reestablish transcriptional regulation of 5-HT1A autoreceptors could provide a more robust and sustained antidepressant response.
Human Brain-Derived Aβ Oligomers Bind to Synapses and Disrupt Synaptic Activity in a Manner That Requires APP
Authors: Wang Zemin , Jackson Rosemary J. , Hong Wei , Taylor Walter M. , Corbett Grant T. , Moreno Arturo , Liu Wen , Li Shaomin , Frosch Matthew P. , Slutsky Inna , Young-Pearse Tracy L. , Spires-Jones Tara L. , Walsh Dominic M. 
Published: December 2017

Compelling genetic evidence links the amyloid precursor protein (APP) to Alzheimer's disease (AD) and several theories have been advanced to explain the relationship. A leading hypothesis proposes that a small amphipathic fragment of APP, the amyloid β-protein (Aβ), self-associates to form soluble aggregates that impair synaptic and network activity. Here, we used the most disease-relevant form of Aβ, protein isolated from AD brain. Using this material, we show that the synaptotoxic effects of Aβ depend on expression of APP and that the Aβ-mediated impairment of synaptic plasticity is accompanied by presynaptic effects that disrupt the excitatory/inhibitory (E/I) balance. The net increase in the E/I ratio and inhibition of plasticity are associated with Aβ localizing to synapses and binding of soluble Aβ aggregates to synapses requires the expression of APP. Our findings indicate a role for APP in AD pathogenesis beyond the generation of Aβ and suggest modulation of APP expression as a therapy for AD.SIGNIFICANCE STATEMENTHere, we report on the plasticity-disrupting effects of amyloid β-protein (Aβ) isolated from Alzheimer's disease (AD) brain and the requirement of amyloid precursor protein (APP) for these effects. We show that Aβ-containing AD brain extracts block hippocampal LTP, augment glutamate release probability, and disrupt the excitatory/inhibitory balance. These effects are associated with Aβ localizing to synapses and genetic ablation of APP prevents both Aβ binding and Aβ-mediated synaptic dysfunctions. Our results emphasize the importance of APP in AD and should stimulate new studies to elucidate APP-related targets suitable for pharmacological manipulation.
Instantáneas de un burócrata cultural: el oficio de promover el teatro durante “el milagro mexicano”
Authors: Palacios Paulo César León 
Published: December 2017

El artículo aborda la gestión de Miguel Álvarez Acosta en la Dirección de Relaciones Culturales de la Secretaría de Relaciones Exteriores (sre) de México (1959–1970). Se analiza su relación con los artistas, así como sus ideas acerca del teatro y la cultura en el contexto del “milagro mexicano” (1940–1968). Para ello, el texto usa la categoría de burocracia cultural y la vincula con las discusiones sobre caciquismo e intermediación. La principal conclusión es que el trabajo de Álvarez Acosta por un teatro moderno obedecía a las reglas del juego políticas, que consistían en una mezcla de racionalización burocrática, nacionalismo y clientelismo.
The article analyzes the career of Miguel Álvarez Acosta during his tenure as the Mexican Foreign Affairs Secretary (sre) (1959–1970) in the office of Cultural Relations. His relationships with the artists and his ideas about art and culture are explored in depth and contextualized within “the Mexican miracle” (1940–1968). This essay employs the concept of cultural bureaucracy and ties it to discussions about bossism and broker mediation. The main conclusion is that Álvarez Acosta’s support for a modern theater was bound to the rules of political games; a blend of bureaucratic rationalization, nationalism and clientelism.
“If they are useful, why expel them?” Las Hermanas de la Caridad and Religious Medical Authority in Mexico City Hospitals, 1861–1874
Authors: O’Brien Elizabeth 
Published: December 2017

The expulsion of las Hermanas de la Caridad was a highly contentious battle in Mexico’s nineteenth-century war between Church and State. Las Hermanas—who staffed and administrated Mexico City’s hospitals for three decades (1844–1874)—are generally portrayed as the adventitious victims of President Lerdo de Tejada’s attacks on religion. Using records from Mexico City’s Secretary of Health archive, this article argues that public health officials were a major force behind the expulsion, and that the sisters were ultimately ousted not just because they symbolized the rising influence of Vincentians in Mexico, but also because their medical and administrative autonomy represented a threat to scientific and state authorities.
En la guerra decimonónica mexicana entre la Iglesia y el Estado, la expulsión de las Hermanas de la Caridad generó una contienda sumamente disputada. Las Hermanas—que habían administrado y conformado el personal de los hospitales de la ciudad de México durante tres décadas (1844–1874)—suelen ser presentadas como las víctimas adventicias de los ataques del presidente Lerdo de Tejada contra la religión. Con base en registros del archivo de la Secretaría de Salud de la ciudad de México, este artículo sostiene que los funcionarios públicos del campo de la salud constituyeron una fuerza importante detrás de la expulsión y que, en última instancia, se echó a las Hermanas no sólo porque simbolizaban la influencia creciente de los vicentinos en México, sino también porque su autonomía médica y administrativa representaba una amenaza para las autoridades científicas y estatales.
Transcriptional Profiling of Saccharomyces cerevisiae Reveals the Impact of Variation of a Single Transcription Factor on Differential Gene Expression in 4NQO, Fermentable, and Nonfermentable Carbon Sources
Authors: Rong-Mullins Xiaoqing , Ayers Michael C , Summers Mahmoud , Gallagher Jennifer E G 
Published: December 2017

Abstract
Cellular metabolism can change the potency of a chemical’s tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer different levels of resistance to 4NQO in Saccharomyces cerevisiae. To study how different Yrr1 alleles regulate gene expression leading to resistance, transcriptomes of three isogenic S. cerevisiae strains carrying different Yrr1 alleles were profiled via RNA sequencing (RNA-Seq) and chromatin immunoprecipitation coupled with sequencing (ChIP-Seq) in the presence and absence of 4NQO. In response to 4NQO, all alleles of Yrr1 drove the expression of SNQ2 (a multidrug transporter), which was highest in the presence of 4NQO resistance-conferring alleles, and overexpression of SNQ2 alone was sufficient to overcome 4NQO-sensitive growth. Using shape metrics to refine the ChIP-Seq peaks, Yrr1 strongly associated with three loci including SNQ2. In addition to a known Yrr1 target SNG1, Yrr1 also bound upstream of RPL35B; however, overexpression of these genes did not confer 4NQO resistance. RNA-Seq data also implicated nucleotide synthesis pathways including the de novo purine pathway, and the ribonuclease reductase pathways were downregulated in response to 4NQO. Conversion of a 4NQO-sensitive allele to a 4NQO-resistant allele by a single point mutation mimicked the 4NQO-resistant allele in phenotype, and while the 4NQO resistant allele increased the expression of the ADE genes in the de novo purine biosynthetic pathway, the mutant Yrr1 increased expression of ADE genes even in the absence of 4NQO. These same ADE genes were only increased in the wild-type alleles in the presence of 4NQO, indicating that the point mutation activated Yrr1 to upregulate a pathway normally only activated in response to stress. The various Yrr1 alleles also influenced growth on different carbon sources by altering the function of the mitochondria. Hence, the complement to 4NQO resistance was poor growth on nonfermentable carbon sources, which in turn varied depending on the allele of Yrr1 expressed in the isogenic yeast. The oxidation state of the yeast affected the 4NQO toxicity by altering the reactive oxygen species (ROS) generated by cellular metabolism. The integration of RNA-Seq and ChIP-Seq elucidated how Yrr1 regulates global gene transcription in response to 4NQO and how various Yrr1 alleles confer differential resistance to 4NQO. This study provides guidance for further investigation into how Yrr1 regulates cellular responses to 4NQO, as well as transcriptomic resources for further analysis of transcription factor variation on carbon source utilization.
The Cumulative Effect of Manure on a Festuca Rubra Grasslands for 15 Years
Authors: VAIDA Ioana , ROTAR Ioan , PACURAR Florin 
Published: December 2017

Over time, studies of the vegetation of natural grasslands have evolved from simple observations to rigorous research. Feed obtained from natural meadows without major technological imputations is mediocre in quality, and pastures in a rather advanced state of degradation. The main purpose of this paper is the ecological and fodder evaluation of the Festuca rubra type of grasslands in the Apuseni Mountains based on the floristic composition and the productivity of these grasslands. The experience was carried out in Ghetari village, Apuseni Mountains, which was located in 2001, following the method of randomized blocks with 4 experimental variants in 4 rehearsals. Each variant were fertilized with organic inputs using the same quantities each year. In the paper we use the results obtained after 15 years of experience. The application of organic fertilizers caused significant changes in the canopy causing changes in the dominance of the type of grassland. The livestock manure applied on the studied grassland has increases significant yield production at all variants of fertilization.In this paper it was shown on which species the productivity of the grassland increased from the control variant to the fertilized variant with the maximum dose. Regarding the analysis of the phytodiversity, this tells us if the number of species is reduced in all the treatments, 10 t/ha of manure leads to an increase in phytocoenosis equivalence and implicitly to the Shannon index.
Technogenic Ecological Sequences in Tailing Pond from Căpușu Mare, Built between 1975-1981
Authors: MAXIM Aurel , ȘANDOR Mignon , OPINCARIU Adriana , MIHALESCU Lucia , ROMAN Vasile , BÎLC Larisa 
Published: December 2017

In Romania, all actions related to mining management are found in “Strategy of the mining industry for 2012-2035”. Today, numerous tailing ponds have remained outside rehabilitation operations, and they are becoming more natural, as is the case of the tailing pond in Capusu Mare. The vegetation study was conducted in 2015, at the tailing pond III in Căpușu Mare that was operational between 1975-1981. The following phytopopulation and phytocenotic indices were calculated: presence, frequency, class of presence, abundance-dominance and average abundance-dominance (ADm) of species. Floral studies show the presence of 40 plant species. The wood species with the highest mean dominant abundance are Hippophäe rhamnoides (14.78%) and Salix alba (10.55%), and of herbaceous species stands Phragmites communis with 7.49%. After 34 years from the heap closure, the degree of vegetation coverage is 77%. Wood species occupy about 32% of the heap surface.
Decision-Making Capacity for Chemotherapy and Associated Factors in Newly Diagnosed Patients with Lung Cancer
Authors: Ogawa Asao , Kondo Kyoko , Takei Hiroyuki , Fujisawa Daisuke , Ohe Yuichiro , Akechi Tatsuo 
Published: December 2017

AbstractBackgroundThe objective of this study was to assess decision-making capacity in patients newly diagnosed with lung cancer, clinical factors associated with impaired capacity, and physicians’ perceptions of patients’ decision-making capacity.Materials and MethodsWe recruited 122 patients newly diagnosed with lung cancer. One hundred fourteen completed the assessment. All patients were receiving a combination of treatments (e.g., chemotherapy, chemo-radiotherapy, or targeted therapy). Decision-making capacity was assessed using the MacArthur Competence Tool for Treatment. Cognitive impairment, depressive symptoms, and frailty were also evaluated. Physicians’ perceptions were compared with the ascertainments.ResultsTwenty-seven (24%, 95% confidence interval [CI], 16–31) patients were judged to have incapacity. Clinical teams had difficulty in judging six (22.2%) patients for incapacity. Logistic regression identified frailty (odds ratio, 3.51; 95% CI, 1.13–10.8) and cognitive impairment (odds ratio, 5.45; 95% CI, 1.26–23.6) as the factors associated with decision-making incapacity. Brain metastasis, emphysema, and depression were not associated with decision-making incapacity.ConclusionA substantial proportion of patients diagnosed with lung cancer show impairments in their capacity to make a medical decision. Assessment of cognitive impairment and frailty may provide appropriate decision-making frameworks to act in the best interest of patients.Implications for PracticeDecision-making capacity is the cornerstone of clinical practice. A substantial proportion of patients with cancer show impairments in their capacity to make a medical decision. Assessment of cognitive impairment and frailty may provide appropriate decision-making frameworks to act in the best interest of patients.
Supporting Young Children’s Learning in a Dramatic Play Environment
Authors: Hostettler Scharer Janine 
Published: December 2017

<div class="page" title="Page 1"><div class="section"><div class="layoutArea"><div class="column"><p><span>Vygotsky and his followers believe that make- believe play is the leading activity in preschool, kindergarten, and the early primary grades. Play-based curricula are common in early childhood classrooms today, but what does this look like in practice? Does it mean that children get some time for free play in centres between blocks of direct instruction? What could learning in a play-learning environment look like? Prospective ECE teachers often show difficulty integrating play for learning. In their experiences, play is often something to do in between their guided instruction. In this paper I challenge those beliefs and show, based on an example, how learning and teaching are possible through play when the whole classroom is transformed into a museum. Teachers will not only be addressing curricular goals, but also encouraging imagination, creativity, social development, and many other learning goals at the same time. Based on Vygotsky’s theory on play, I present ways to enrich, support, and foster play while learning within a play-learning environment. </span></p></div></div></div></div>
El potencial de los activos culturales asociados con la actividad turística como factor de desarrollo local en el barrio Getsemaní, Cartagena de Indias.
Authors: Escobar Nieves Alfonso , Beltrán Vargas Merly , Bonza Quesada Esthella 
Published: December 2017

El uso de patrimonio e identidad cultural y su influencia en el desarrollo de un territorio han sido abordados tanto en la teoría como en la práctica en países de América Latina, enriqueciéndose con experiencias significativas de algunos ejemplos internacionales que reivindican la identidad cultural y valorizan el patrimonio local. Por lo anterior, resulta útil analizar el caso del barrio Getsemaní, ubicado en Cartagena de Indias, inscrito por la UNESCO en la Lista del Patrimonio de la Humanidad.La metodología empleada corresponde al estudio de caso y combina técnicas cualitativas y cuantitativas. La investigación analiza el potencial de Getsemaní para contribuir al desarrollo local a partir del reconocimiento y la visibilización de los bienes y manifestaciones culturales.En ese orden, se realiza una caracterización general del barrio a partir del análisis de la documentación presente, de los registros estadísticos oficiales disponibles y de los resultados de entrevistas y encuestas que permitieron complementar información sobre temas de interés. Así mismo, se aportan evidencias del potencial turístico y el carácter activo del patrimonio cultural de Getsemaní. En este sentido, a partir del sustento complementario de la teoría del desarrollo endógeno con el modelo de desarrollo turístico imperante, se propone potenciar las características singulares del patrimonio cultural de Getsemaní por medio de la idea de un conjunto interpretativo que busca potenciar la gestión turística por parte de la comunidad local y demostrar el valor diferencial de Getsemaní como destino turístico cultural. De esta manera, el desarrollo se logra no solo siendo un receptor pasivo, sino que también se alcanza a partir de la formación de un proceso emprendedor y de innovación local basado en una especificidad cultural, del patrimonio material e inmaterial y territorial.Es importante resaltar el valor estratégico, los riesgos y las oportunidades asociados con la salvaguardia del patrimonio cultural y el desarrollo del turismo. Las comunidades deben liderar la gestión en el marco de la solidaridad y la cooperación para garantizar su supervivencia en un mundo globalizado, con participación activa de los distintos actores gubernamentales y privados del sector.
EFECTO ALELOPÁTICO DE ESCOBA DE BRUJA (Moniliophthora perniciosa Stahel.) INOCULADAS EN SIETE ESPECIES DE MALEZAS
Authors: Vera Chang Jaime Fabian , Vallejo Torres Christian , Garcés Fiallos Felipe Rafael , Sánchez Mora Fernando David , Jácome López Germán , Vásconez Montúfar Gregorio Humberto , Vera Barahona Jaime Set , Ramos Remache Rommel 
Published: December 2017

El estudio consistió en evaluar el efecto del extracto acuoso obtenido de ramas de cacao sanas y extracto etanolico de ramas infectadas por el hongo Moniliophthora perniciosa Stahel, sobre el follaje de siete malezas (cinco de hoja ancha y dos de hoja angosta). El porcentaje de necrosis en hojas se determinó en base a una escala nominal (0-7). Para las malezas de hoja angosta el mejor tratamiento T6 al día 15 presentó una severidad de 4.38%, al día 30 este valor desciende 4.29% para finalmente al día 45 dar un valor de 3.44%, pese a que la planta en los primeros día tiene una severidad alta, esta obtiene resistencia a medida que pasan los días de evaluación, así mismo el T2 al día 15 tiene una severidad 4.24% aumentando este valor a los 30 días a 4.31%, pero a los 45 días este valor disminuye a 4.16%. Para las malezas de hoja ancha el mejor tratamiento los primeros 15 días fue el T1 4.05% este valor descendiendo a los 30 días 4.01% y se mantiene hasta el día 45, así mismo la severidad en los 15 días para los T3 (3.90%) y T5 (4.01), después a los 30 días aumentó ligeramente T3 (3.93%) y T5 (4.06), pero a los 45 días decrece T3 (3.88%) y T5 (3.25), en ambas malezas de hoja ancha y angosta se asume que la planta los primeros días no tiene la capacidad de resistir el ataque causado por los extractos, pero a partir de los días 30 y 45, este obtiene resistencia y se recupera posiblemente mediante mecanismos quimiotaxonómicos o fisiológicos.
Valeurs d’usage des ligneux utilisés en agroforesterie : les cacaoyères du Centre-Cameroun
Authors: Jagoret Patrick , Kwesseu Jacques , Messie Charly Anicet , Michel Isabelle , Malézieux Éric 
Published: December 2017

En zone tropicale humide, la valeur des systèmesagroforestierspour lesagriculteurs est souvent difficile à évaluer. À partir d’un inventaire floristique réalisé dans 14 cacao- yères agroforestières du Centre-Cameroun, l’article propose leur évaluation participative basée sur la méthode de distribution des cailloux mobilisée pour quantifier la valeur accordée par les agriculteurs à chaque espèce en fonction des usages attribués. Au total, 86 espèces ligneuses ont été inventoriées. Les cinq espèces les plus fré- quentes ont été le safoutier, Dacryodes edulis, avec 18 % des arbres inventoriés, l’avocatier, Persea americana, avec 11 %, le fraké, Terminalia superba, avec 10 %, le figuier, Ficus mucoso, avec 5 %, et Albizia adianthifolia, avec 3 %. Vingt-deux espèces (26 %) n’ont eu aucun usage pour les agri- culteursqui ont défini sept usagesdifférents pour lesautresespècesinventoriées. Parmi cesespèces, la valeur d’usage la plusélevée a été attribuée au cacaoyer (20 %). Ensuite, par ordre décroissant, les cinq espèces qui ont eu les valeurs d’usage les plus élevées ont été l’ayous, Triplochyton scleroxylon, le sapelli, Entandrophragma cylindricum, le safoutier, Dacryodes edulis, le palmier à huile, Elaeis guineensis, et le manguier, Mangifera indica. Le profil d’usage des cacaoyères a montré qu’elles sont princi- palement pilotées par les agriculteurs pour la vente de produits ligneux et non ligneux, l’autoconsommation de produitsnon ligneux et de produits médicinaux. Les résultats indiquent que les innovations techniques visant à améliorer lessystèmesagroforestiers cacaoyersdoivent tenir compte de leur com- plexité et de leur plurifonctionnalité.