bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time Estimation in Chronic Insomnia Sufferers
Authors: Rioux Isabelle , Tremblay Sébastien , Bastien Célyne H. 
Published: February 2017

Abstract

Study Objective:
The objective of the present study is 2-fold: (1) compare the time-estimation performance of chronic insomnia sufferers to that of good sleepers and (2) evaluate the severity of the sleep complaint in order to assess its association with the time-estimation performance.

Design:
Between subjects design.

Participants:
The sample included 11 individuals suffering from chronic primary insomnia (7 women and 4 men, mean age = 44.64 years, SD = 12.71) and 11 good sleepers (5 women and 6 men, mean age = 48.00 years, SD = 7.86).

Interventions:
N/A.

Measurements and Results:
All participants completed a time-estimation task, namely a finger-tapping task. The results indicate no significant between-group differences on time-estimation data, as well as no significant relationship between severity of insomnia complaint and estimation of time.

Conclusion:
These results suggest that the tendency to misestimate sleep difficulties is not linked to impaired time estimation-specific processes in insomnia sufferers, as measured with the present task.
How activity dependent feedback inhibition may maintain head direction signals in mouse presubiculum
Authors: Simonnet Jean , Nassar Mérie , Stella Federico , Cohen Ivan , Mathon Bertrand , Boccara Charlotte N. , Miles Richard , Fricker Desdemona 
Published: February 2017

ABSTRACTOrientation in space is represented in specialized brain circuits. Persistent head direction signals are transmitted from anterior thalamus to the presubiculum, but the identity of the presubicular target neurons, their connectivity and function in local microcircuits are unknown. Here we examine how thalamic afferents recruit presubicular principal neurons and Martinotti interneurons and the ensuing synaptic interactions between these cells. Pyramidal neuron activation of Martinotti cells in superficial layers is strongly facilitating such that high frequency head directional stimulation efficiently unmutes synaptic excitation. Martinotti cell feedback plays a dual role: precisely timed spikes may not inhibit the firing of in-tune head direction cells, while exerting lateral inhibition. Autonomous attractor dynamics emerge from a modeled network implementing wiring motifs and timing sensitive synaptic interactions in the pyramidal - Martinotti cell feedback loop. This inhibitory microcircuit is therefore tuned to refine and maintain head direction information in the presubiculum.
Cryptic diversity and discordance in single‐locus species delimitation methods within horned lizards (Phrynosomatidae: Phrynosoma)
Authors: Blair Christopher , Bryson Robert W. 
Published: February 2017

AbstractBiodiversity reduction and loss continues to progress at an alarming rate, and thus, there is widespread interest in utilizing rapid and efficient methods for quantifying and delimiting taxonomic diversity. Single‐locus species delimitation methods have become popular, in part due to the adoption of the DNA barcoding paradigm. These techniques can be broadly classified into tree‐based and distance‐based methods depending on whether species are delimited based on a constructed genealogy. Although the relative performance of these methods has been tested repeatedly with simulations, additional studies are needed to assess congruence with empirical data. We compiled a large data set of mitochondrial ND4 sequences from horned lizards (Phrynosoma) to elucidate congruence using four tree‐based (single‐threshold GMYC, multiple‐threshold GMYC, bPTP, mPTP) and one distance‐based (ABGD) species delimitation models. We were particularly interested in cases with highly uneven sampling and/or large differences in intraspecific diversity. Results showed a high degree of discordance among methods, with multiple‐threshold GMYC and bPTP suggesting an unrealistically high number of species (29 and 26 species within the P. douglasii complex alone). The single‐threshold GMYC model was the most conservative, likely a result of difficulty in locating the inflection point in the genealogies. mPTP and ABGD appeared to be the most stable across sampling regimes and suggested the presence of additional cryptic species that warrant further investigation. These results suggest that the mPTP model may be preferable in empirical data sets with highly uneven sampling or large differences in effective population sizes of species.
Homology‐guided mutational analysis reveals the functional requirements for antinociceptive specificity of collapsin response mediator protein 2‐derived peptides
Authors: Moutal Aubin , Li Wennan , Wang Yue , Ju Weina , Luo Shizhen , Cai Song , François‐Moutal Liberty , Perez‐Miller Samantha , Hu Jackie , Dustrude Erik T , Vanderah Todd W , Gokhale Vijay , Khanna May , Khanna Rajesh 
Published: February 2017

Background and PurposeN‐type voltage‐gated calcium (Cav2.2) channels are critical determinants of increased neuronal excitability and neurotransmission accompanying persistent neuropathic pain. Although Cav2.2 channel antagonists are recommended as first‐line treatment for neuropathic pain, calcium‐current blocking gabapentinoids inadequately alleviate chronic pain symptoms and often exhibit numerous side effects. Collapsin response mediator protein 2 (CRMP2) targets Cav2.2 channels to the sensory neuron membrane and allosterically modulates their function. A 15‐amino‐acid peptide (CBD3), derived from CRMP2, disrupts the functional protein–protein interaction between CRMP2 and Cav2.2 channels to inhibit calcium influx, transmitter release and acute, inflammatory and neuropathic pain. Here, we have mapped the minimal domain of CBD3 necessary for its antinociceptive potential.Experimental ApproachTruncated as well as homology‐guided mutant versions of CBD3 were generated and assessed using depolarization‐evoked calcium influx in rat dorsal root ganglion neurons, binding between CRMP2 and Cav2.2 channels, whole‐cell voltage clamp electrophysiology and behavioural effects in two models of experimental pain: post‐surgical pain and HIV‐induced sensory neuropathy induced by the viral glycoprotein 120.Key ResultsThe first six amino acids within CBD3 accounted for all in vitro activity and antinociception. Spinal administration of a prototypical peptide (TAT‐CBD3‐L5M) reversed pain behaviours. Homology‐guided mutational analyses of these six amino acids identified at least two residues, Ala1 and Arg4, as being critical for antinociception in two pain models.Conclusions and ImplicationsThese results identify an antinociceptive scaffold core in CBD3 that can be used for development of low MW mimetics of CBD3.Linked ArticlesThis article is part of a themed section on Recent Advances in Targeting Ion Channels to Treat Chronic Pain. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.12/issuetoc
Soil carbon sequestration due to post‐Soviet cropland abandonment: estimates from a large‐scale soil organic carbon field inventory
Authors: Wertebach Tim‐Martin , Hölzel Norbert , Kämpf Immo , Yurtaev Andrey , Tupitsin Sergey , Kiehl Kathrin , Kamp Johannes , Kleinebecker Till 
Published: February 2017

AbstractThe break‐up of the Soviet Union in 1991 triggered cropland abandonment on a continental scale, which in turn led to carbon accumulation on abandoned land across Eurasia. Previous studies have estimated carbon accumulation rates across Russia based on large‐scale modelling. Studies that assess carbon sequestration on abandoned land based on robust field sampling are rare. We investigated soil organic carbon (SOC) stocks using a randomized sampling design along a climatic gradient from forest steppe to Sub‐Taiga in Western Siberia (Tyumen Province). In total, SOC contents were sampled on 470 plots across different soil and land‐use types. The effect of land use on changes in SOC stock was evaluated, and carbon sequestration rates were calculated for different age stages of abandoned cropland. While land‐use type had an effect on carbon accumulation in the topsoil (0–5 cm), no independent land‐use effects were found for deeper SOC stocks. Topsoil carbon stocks of grasslands and forests were significantly higher than those of soils managed for crops and under abandoned cropland. SOC increased significantly with time since abandonment. The average carbon sequestration rate for soils of abandoned cropland was 0.66 Mg C ha−1 yr−1 (1–20 years old, 0–5 cm soil depth), which is at the lower end of published estimates for Russia and Siberia. There was a tendency towards SOC saturation on abandoned land as sequestration rates were much higher for recently abandoned (1–10 years old, 1.04 Mg C ha−1 yr−1) compared to earlier abandoned crop fields (11–20 years old, 0.26 Mg C ha−1 yr−1). Our study confirms the global significance of abandoned cropland in Russia for carbon sequestration. Our findings also suggest that robust regional surveys based on a large number of samples advance model‐based continent‐wide SOC prediction.
Exploring Greenhouse Gas‐Mitigation Strategies in Chinese Eco‐Industrial Parks by Targeting Energy Infrastructure Stocks
Authors: Guo Yang , Tian Jinping , Chertow Marian , Chen Lujun 
Published: February 2017

SummaryChina has more than 1,500 industrial parks, which, collectively, play a crucial role in facilitating industrialization and urbanization. A key characteristic of these parks is that most rely on shareable energy infrastructure, an efficient configuration that can also deliver substantial and sustainable reductions in greenhouse gas (GHG) emissions. This study offers strategies for mitigating GHG emissions from Chinese industrial parks. We focus on extensive data collection for the 106 industrial parks listed in the national demonstration eco‐industrial park (EIP) program. In doing so, we carefully examine the evolution of 608 serviceable energy infrastructure units by vintage year, fuel type, energy output, and technologies of combined heat and power units. We assess direct GHG emissions from both energy infrastructure and the parks, and then identify the features and driving forces of energy infrastructure development in the EIPs. We also offer recommendations for ways to mitigate the GHG emissions from these industrial parks. The energy infrastructure stocks in Chinese EIPs are characterized by heavy coal dependence (87% of capacity) and high ratios of direct GHG emissions versus the total direct emissions of the park (median value: 75.2%). These findings establish a baseline from which both technology and policy decisions can then be made in an informed way.
Mediator of DNA damage checkpoint protein 1 (MDC1) as a prognostic marker for patients with oral squamous cell carcinoma
Authors: Dave Jigna Harshadbhai , Vora Hemangini Hasit , Ghosh Nandita Radhabinod , Trivedi Trupti Indravadan 
Published: February 2017

BackgroundThe mediator of DNA damage checkpoint protein 1 (MDC1) is involved in the regulation of cell cycle checkpoints and recruitment of several repair proteins to the site of DNA double‐stranded breaks (DSBs). This study aimed to correlate the expression of MDC1 protein with clinicopathological parameters and to evaluate its prognostic significance in patients with oral squamous cell carcinoma (OSCC).MethodsMDC1 protein expression was evaluated immunohistochemically from untreated 100 patients with OSCC using modified H‐score method. The association of MDC1 immunostaining was evaluated with clinicopathological parameters and disease outcome using univariate and multivariate survival analysis for relapse‐free survival (RFS) and overall survival (OS).ResultsIncidence of nuclear and cytoplasmic expression of MDC1 protein was 85% & 92%, respectively. Strong nuclear MDC1 protein expression was found to be significantly correlated with lymph node metastasis (P = 0.032). For RFS, Kaplan–Meier survival analysis demonstrated that presence of metastatic lymph node (P = 0.001), lymphatic permeation (P = 0.020), and nuclear MDC1 (P = 0.005) remained significant risk predictors. In multivariate survival analysis, nuclear MDC1 (P = 0.027) entered at step 2 after presence of metastatic lymph node (P = 0.002) at step 1 for predicting reduced RFS. In relation to treatment, OSCC patients exhibiting weak expression of nuclear MDC1 protein were benefited significantly when treated with surgery followed by radiation therapy (P = 0.001).ConclusionThus, this study showed that MDC1 protein expression could be used as a prognostic marker in predicting relapse‐free survival in patients with OSCC. OSCC patients expressing weak MDC1 protein could be benefited by adjuvant radiotherapy instead chemo‐radiotherapy.
Associations of gender and a proxy of female menopausal status with histological features of drug‐induced liver injury
Authors: Suzuki Ayako , Barnhart Huiman , Gu Jiezhun , Bonkovsky Herbert L. , Tillmann Hans L. , Fontana Robert J. , Kleiner David E. ,   Drug‐induced Liver Injury Network (DILIN)
Published: February 2017

AbstractBackground & AimGender and menopause may contribute to type and severity of drug‐induced liver injury (DILI) by influencing host responses to injury. The aim of this study was to assess the associations of gender and female age 50 [a proxy of menopause] with histological features of liver injury in 212 adults enrolled in the Drug‐Induced Liver Injury Network (DILIN) registry.MethodsAll participants had a causality score of at least ‘probable’, a liver biopsy within 30 days of DILI onset, and no prior chronic liver disease. Biochemical and histological injury types were classified as hepatocellular or cholestatic/mixed injury. The cohort was divided into three gender/age categories: men (41.0%), women <50 years (27.4%) and women ≥50 years of age (31.6%). Interaction of gender and age category (≥50 or not) was assessed.ResultsHepatocellular injury was more prevalent in women <50 years vs. others (P=.002). After adjusting for biochemical injury types, black race and possible ageing effects, more severe interface hepatitis was noted in biopsies of women <50 years compared to those of men and women ≥50 years (P=.009 and P=.055 respectively). Compared to those of men, biopsies of women showed greater plasma cell infiltration, hepatocyte apoptosis, hepatocyte rosettes and lobular disarray but less iron‐positive hepatocytes and histological cholestasis (P<.05). These associations persisted after excluding cases of amoxicillin/clavulanic acid, anabolic steroids or nitrofurantoin DILI which showed gender‐specific distributions.ConclusionGender and a proxy of menopause were associated with various features of inflammation and injury in DILI.
Phenytoin‐induced gingival overgrowth caused by death receptor pathway malfunction
Authors: Takeuchi R , Matsumoto H , Arikawa K , Taguchi C , Nakayama R , Nasu I , Hiratsuka K 
Published: February 2017

ObjectiveIn this study, we investigated the role of phenytoin (PHT) in death receptor‐induced apoptosis of gingival fibroblasts to clarify the mechanism of PHT‐induced gingival overgrowth.MethodsHuman gingival fibroblasts were cultured to semiconfluence and treated with PHT (0.025, 0.1, 0.25, and 1.0 μM) for 48 h, and then, the apoptotic cell numbers were relatively determined by absorptiometry. After 24 h of 0.25 μM PHT treatment, caspase activity was measured by absorptiometry, apoptotic and cell cycle phase distribution was analyzed by flow cytometry, expression levels of apoptotic genes were quantified by real‐time qPCR, and expression of apoptotic proteins was detected by Western blot analysis. After 48 h of 0.25 μM PHT treatment, appearance of apoptotic cells was detected by TUNEL assay.ResultsPHT treatment decreased the proportion of apoptotic cells in gingival fibroblasts compared to a serum‐free control culture in response to the protein changes as follows: PHT upregulated c‐FLIP and, in turn, downregulated FADD, caspase‐8, and caspase‐3; PHT upregulated c‐IAP2 and downregulated TRAF2; PHT downregulated caspase‐9 and caspase‐3 via decreased RIPK1 activity and increased Bcl‐2 activity.ConclusionPHT‐induced gingival overgrowth may result from the above‐mentioned mechanisms involving apoptosis inhibition in gingival fibroblasts.
Sexual reproduction, sporophyte development and molecular variation in the model moss Physcomitrella patens: introducing the ecotype Reute
Authors: Hiss Manuel , Meyberg Rabea , Westermann Jens , Haas Fabian B. , Schneider Lucas , Schallenberg‐Rüdinger Mareike , Ullrich Kristian K. , Rensing Stefan A. 
Published: February 2017

SummaryRich ecotype collections are used for several plant models to unravel the molecular causes of phenotypic differences, and to investigate the effects of environmental adaption and acclimation. For the model moss Physcomitrella patens collections of accessions are available, and have been used for phylogenetic and taxonomic studies, for example, but few have been investigated further for phenotypic differences. Here, we focus on the Reute accession and provide expression profiling and comparative developmental data for several stages of sporophyte development, as well as information on genetic variation via genomic sequencing. We analysed cross‐technology and cross‐laboratory data to define a confident set of 15 mature sporophyte‐specific genes. We find that the standard laboratory strain Gransden produces fewer sporophytes than Reute or Villersexel, although gametangia develop with the same time course and do not show evident morphological differences. Reute exhibits less genetic variation relative to Gransden than Villersexel, yet we found variation between Gransden and Reute in the expression profiles of several genes, as well as variation hot spots and genes that appear to evolve under positive Darwinian selection. We analyzed expression differences between the ecotypes for selected candidate genes in the GRAS transcription factor family, the chalcone synthase family and in genes involved in cell wall modification that are potentially related to phenotypic differences. We confirm that Reute is a P. patens ecotype, and suggest its use for reverse‐genetics studies that involve progression through the life cycle and multiple generations.
D/Ecrire une ville: Géocritique de Nouméa à partir de brèches romanesques
Authors: Banaré Eddy 
Published: February 2017

Abstract
This article explores the way literature informs and is informed by the perception of urban postcolonial space. Chroniques De La Mauvaise Herbe, the first novel of Vincent Vuibert (2013), marks a new threshold in the Francophone oceanian literary field, at first by the influences claimed by the author and then by the representation renewed of Noumea. Among these influences is the contemporary U.S. thriller in which Vuibert renews an aesthetics of the marginality to stack them in the colonial and postcolonial segmentarities. His characters seem to evolve along borders established from regimes of political, historic and economic powers, which they undergo or also try to thwart. On the other hand, this novel joins in a discursive tradition centred on the city inaugurated by the first Kanak political militancy of the 1970s and which was strengthened in the literary field with the signature of the political agreements of 1988 and 1998. It is a question here of showing how the novel draws routes in Noumea who are so many ways to describe oppositions and encounters.
Diagnosis of sphingolipidoses: a new simultaneous measurement of lysosphingolipids by LC-MS/MS
Authors: Polo Giulia , Burlina Alessandro P. , Kolamunnage Thilini B. , Zampieri Michele , Dionisi-Vici Carlo , Strisciuglio Pietro , Zaninotto Martina , Plebani Mario , Burlina Alberto B. 
Published: February 2017

AbstractBackground:Lysosphingolipids (LysoSLs) are derivatives of sphingolipids which have lost the amide-linked acyl chain. More recently, LysoSLs have been identified as storage compounds in several sphingolipidoses, including Gaucher, Fabry and Niemann-Pick diseases. To date, different methods have been developed to measure each individual lysosphingolipid in plasma. This report describes a rapid liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) assay for simultaneous quantification of several LysoSLs in plasma.Methods:We analyzed the following compounds: hexosylsphingosine (HexSph), globotriaosylsphingosine (LysoGb3), lysosphingomyelin (LysoSM) and lysosphingomyelin-509 (LysoSM-509). The sample preparation requires only 100 μL of plasma and consists of an extraction with a mixture of MeOH/acetone/HResults:The method validation showed high sensitivity, an excellent accuracy and precision. Reference ranges were determined in healthy adult and pediatric population. The results demonstrate that the LC-MS/MS method can quantify different LysoSLs and can be used to identify patients with Fabry (LysoGb3), Gaucher and Krabbe (HexSph) diseases, prosaposine deficiency (LysoGb3 and HexSph), and Niemann-Pick disease types A/B and C (LysoSM and LysoSM-509).Conclusions:This LC-MS/MS method allows a rapid and simultaneous quantification of LysoSLs and is useful as a biochemical diagnostic tool for sphingolipidoses.
Logarithmic Sobolev inequalities for Moebius measures on spheres
Authors: Barthe Franck , Ma Yutao , Zhang Zhengliang 
Published: February 2017

Abstract
In this paper, using the method in [1], i.e., reduce Moebius measures

μ
x
n

{\mu_{x}^{n}}

indexed by

|
x
|

<
1

{|x|<1}

on spheres

S

n
-
1

{S^{n-1}}

(

n
≥
3

{n\geq 3}

) to one-dimensional diffusions on

[
0
,
π
]

{[0,\pi]}

, we obtain that the optimal Poincaré constant is not greater than

2

n
-
2

{\frac{2}{n-2}}

and the optimal logarithmic Sobolev constant denoted by

C
LS

⁢

(

μ
x
n

)

{C_{\rm LS}(\mu_{x}^{n})}

behaves like

1
n

⁢

log
⁡

(

1
+

1

1
-

|
x
|

)

{\frac{1}{n}\log(1+\frac{1}{1{-}|x|})}

. As a consequence, we claim that logarithmic Sobolev inequalities are strictly stronger than

L
2

{L^{2}}

-transportation-information inequalities.
A Compact Pentagonal Ring CPW-Fed Zeroth Order Resonating Antenna with Gain Enhancement
Authors: Gupta Ashish , Chaudhary Raghvendra Kumar 
Published: February 2017

Abstract
In this work, a pentagonal ring CPW-fed zeroth order resonating (ZOR) antenna with gain enhancement is proposed. Gain enhancement is achieved by means of electromagnetic bandgap structures (EBG) loaded on patch. The proposed antenna is operated in series resonant mode, therefore resonance frequency is dominated by series parameters of the conventional CRLH transmission line. The antenna comprises of a circular patch which is imposed by interdigital capacitor and EBG structures. Meandered line inductor is connected between patch and pentagonal ground plane in order to ensure miniaturization using CRLH resonant approach. Measurement results shows that proposed antenna offers ZOR resonance at 2.7 GHz with 4.81 %, 10 dB fractional bandwidth. The antenna offers simulated gain of 1.63 dBi and 80.6 % radiation efficiency at ZOR frequency. Surface current distribution shows that how these CRLH – TL components responsible for origination of ZOR mode. Proposed antenna exhibits excellent radiation patterns with cross polarization level of −53 dB in the boresight direction.