bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Glasgow (Scotland) geotechnical GIS: a desk study tool
Authors: Entwisle David C. , Culshaw Martin G. , Hulbert Andrew G. , Shelley Wayne A. , Self Suzanne J. , Dobbs Marcus R. 
Published: September 2016

Abstract
Desk study is an essential part of all civil engineering project ground investigations. It is usually a collation and review of existing data and information about a site and, in some cases, the surrounding area, and carried out at an early stage of the ground investigation to inform and guide the ground investigation. It should provide suitable data and information to assess the ground conditions and the implications for the proposed engineering design. A similar approach can be taken to inform local, regional or national government with regard to development and the redevelopment of urban areas where ground investigation data and information are available. This paper describes a spatially defined geotechnical information system (GIS) designed to provide geological, geotechnical and geoenvironmental data and information for Glasgow City Council (Scotland). The system contains three main parts: the geology (bedrock, Quaternary and artificial deposits and the thickness and depth of these deposits); the data are presented as various summary graphs illustrating the variation of various parameters as well as a geotechnical and geoenvironmental database; and tools specifically developed to present the data. As undermining is a hazard in part of Glasgow, a dataset showing the distribution of mining is included. Further interpretation of the characteristics of the geological units has produced an engineering geological classification which may be used to provide rapid assessment of the engineering geological conditions.
Aberrant JMJD3 Expression Upregulates Slug to Promote Migration, Invasion, and Stem Cell–Like Behaviors in Hepatocellular Carcinoma
Authors: Tang Bo , Qi Guangying , Tang Fang , Yuan Shengguang , Wang Zhenran , Liang Xingsi , Li Bo , Yu Shuiping , Liu Jie , Huang Qi , Wei Yangchao , Zhai Run , Lei Biao , Yu Hongping , Tomlinson Stephen , He Songqing 
Published: September 2016

Abstract
The Jumonji domain–containing chromatin remodeling factor JMJD3 has important roles in development and cancer. Here, we report a pivotal role for JMJD3 in sustaining the phenotype of aggressive hepatocellular carcinomas. Expression levels of JMJD3 in clinical specimens of hepatocellular carcinoma correlated inversely with patient survival. In hepatocellular carcinoma cells, we found that enforcing its overexpression induced epithelial–mesenchymal transition (EMT), invasive migration, stem cell–like traits, and metastatic properties. Conversely, silencing JMJD3 in hepatocellular carcinoma cells overexpressing it inhibited these aggressive phenotypes. Mechanistically, JMJD3 modulated H3K27me3 in the SLUG gene promoter, a histone mark associated with active SLUG transcription. SLUG silencing blocked JMJD3-induced EMT, stemness, and metastasis. Furthermore, SLUG expression in hepatocellular carcinoma clinical specimens correlated positively with JMJD3 expression. Our results establish JMJD3 as a critical driver of hepatocellular carcinoma stem cell–like and metastatic behaviors, with implications for prognosis and treatment. Cancer Res; 76(22); 6520–32. ©2016 AACR.
LncRNA HOXA11-AS Promotes Proliferation and Invasion of Gastric Cancer by Scaffolding the Chromatin Modification Factors PRC2, LSD1, and DNMT1
Authors: Sun Ming , Nie Fengqi , Wang Yunfei , Zhang Zhihong , Hou Jiakai , He Dandan , Xie Min , Xu Lin , De Wei , Wang Zhaoxia , Wang Jun 
Published: September 2016

Abstract
Long noncoding RNAs (lncRNA) have been implicated in human cancer but their mechanisms of action are mainly undocumented. In this study, we investigated lncRNA alterations that contribute to gastric cancer through an analysis of The Cancer Genome Atlas RNA sequencing data and other publicly available microarray data. Here we report the gastric cancer–associated lncRNA HOXA11-AS as a key regulator of gastric cancer development and progression. Patients with high HOXA11-AS expression had a shorter survival and poorer prognosis. In vitro and in vivo assays of HOXA11-AS alterations revealed a complex integrated phenotype affecting cell growth, migration, invasion, and apoptosis. Strikingly, high-throughput sequencing analysis after HOXA11-AS silencing highlighted alterations in cell proliferation and cell–cell adhesion pathways. Mechanistically, EZH2 along with the histone demethylase LSD1 or DNMT1 were recruited by HOXA11-AS, which functioned as a scaffold. HOXA11-AS also functioned as a molecular sponge for miR-1297, antagonizing its ability to repress EZH2 protein translation. In addition, we found that E2F1 was involved in HOXA11-AS activation in gastric cancer cells. Taken together, our findings support a model in which the EZH2/HOXA11-AS/LSD1 complex and HOXA11-AS/miR-1297/EZH2 cross-talk serve as critical effectors in gastric cancer tumorigenesis and progression, suggesting new therapeutic directions in gastric cancer. Cancer Res; 76(21); 6299–310. ©2016 AACR.
Meat, Fish, Poultry, and Egg Intake at Diagnosis and Risk of Prostate Cancer Progression
Authors: Wilson Kathryn M. , Mucci Lorelei A. , Drake Bettina F. , Preston Mark A. , Stampfer Meir J. , Giovannucci Edward , Kibel Adam S. 
Published: September 2016

AbstractLittle information exists on diet and prostate cancer progression. We examined the association between intakes of total red meat, processed and unprocessed red meat, poultry, fish, and eggs and prostate cancer recurrence. We conducted a prospective study of 971 men treated with radical prostatectomy for prostate cancer between 2003 and 2010. Men completed a food frequency questionnaire at diagnosis. We used logistic regression to study the association between diet and high-grade or advanced-stage disease. We used Cox models to study the risk of progression [N = 94 events, mainly prostate-specific antigen (PSA) recurrence]. Total red meat intake was marginally associated with risk of high-grade disease [Gleason ≥ 4+3; adjusted OR top vs. bottom quartile: 1.66; 95% confidence interval (CI), 0.93–2.97; Ptrend = 0.05], as was very high intake of eggs (OR top decile vs. bottom quartile: 1.98; 95% CI, 1.08–3.63, Ptrend = 0.08). Well-done red meat was associated with advanced disease (≥pT3; OR top vs. bottom quartile: 1.74, 95% CI, 1.05–2.90; Ptrend = 0.01). Intakes of red meat, fish, and eggs were not associated with progression. Very high poultry intake was inversely associated with progression (HR top decile vs. bottom quartile: 0.19; 95% CI, 0.06–0.63; Ptrend = 0.02). Substituting 30 g/d of poultry or fish for total or unprocessed red meat was associated with significantly lower risk of recurrence. Lower intakes of red meat and well-done red meat and higher intakes of poultry and fish are associated with lower risk of high grade and advanced prostate cancer and reduced recurrence risk, independent of stage and grade. Cancer Prev Res; 9(12); 933–41. ©2016 AACR.
Long-Range Chromatin Interactions Drive Mutant TERT Promoter Activation
Authors: Akıncılar Semih Can , Khattar Ekta , Boon Priscilla Li Shan , Unal Bilal , Fullwood Melissa Jane , Tergaonkar Vinay 
Published: September 2016

Abstract
Cancer-specific TERT promoter mutations (-146C&gt;T and -124C&gt;T) have been linked to reactivation of the epigenetically silenced telomerase reverse transcriptase gene (TERT). Understanding how these single-nucleotide alterations drive TERT reactivation is a fundamental unanswered question and is key for making successful therapeutics. We show that unlike wild-type promoters, recruitment of the transcription factor GABPA specifically to mutant TERT promoters mediates long-range chromatin interaction and enrichment of active histone marks, and hence drives TERT transcription. CRISPR-mediated reversal of mutant TERT promoters, or deletion of its long-range interacting chromatin, abrogates GABPA binding and long-range interactions, leading to depletion of active histone marks, loss of POL2 recruitment, and suppression of TERT transcription. In contrast, de novo introduction of a TERT promoter mutation enables GABPA binding and upregulation of TERT via long-range interactions, acquisition of active histone marks, and subsequent POL2 recruitment. This study provides a unifying mechanistic insight into activation of mutant TERT promoters across various human cancers.
Significance: This study identifies a key mechanism by which cancer-specific mutant TERT promoters cause reactivation of TERT. Because the mechanism uncovered here is not utilized by promoters that drive TERT in normal cells, this mechanism could be exploited to make inhibitors which have the potential to block telomerase function and hence the progression of up to 90% of human cancers. Cancer Discov; 6(11); 1276–91. ©2016 AACR.
See related commentary by Min and Shay, p. 1212.
This article is highlighted in the In This Issue feature, p. 1197
Diagnóstico de dirofilariose oculta pelo teste de ELISA, em cães do Estado de São Paulo
Authors: Larson Maria Helena Matiko Akao , Preterote Margareth , Mirandola Regina Meiko Meiko Sakata 
Published: September 2016

A dirofilariose oculta constitui-se num grande problema diagnóstico e pode estar relacionada a: a) infecções pré-patentes; b) infecções causadas por vermes de um único sexo; c) esterilidade dos parasitas devido à idade ou induzida por ação de drogas; d) resposta imune mediada. Avaliou-se, no presente trabalho, a prevalência de dirofilariose em cães do Estado de São Paulo, através do teste de ELISA (CITER Semi-Quant TM Canine Heartworm Antigen Test Kit), bem como se tentou caracterizar a causa da ausência de microfilaremia circulante, com auxílio de exames subsidiários. De 244 soros examinados, 35 (14,34%) mostraram-se positivos. Destes, 20 apresentaram, além do teste de ELISA positivo, una ou mais alterações (radiográficas /eletrocardiográficas), compatíveis com o diagnóstico de dirofilariose canina; e, nos demais 15 animais, assintomáticos, também soropositivos pelo teste de ELISA, descartou-se a forma de infecção prépatente, pois haviam sido expostos ao risco da infecção há mais de 6 meses, e também a esterilidade dos parasitas conseqüentemente à ação de drogas.
Prevalência de ecto e endoparasitas em camundongos e ratos criados em biotério
Authors: Bressan M. Cecília R. Vieira , Calgaro Gerson Amauri , Alexandre Sandra R. , Marques Thais 
Published: September 2016

Durante um período de 5 anos (1986-1991) foram examinados 229 camundongos e 128 ratos, de ambos os sexos, com idade entre 1 a 2 meses, provenientes de 4 biotérios convencionais (2 de camundongos e 2 de ratos), e de um biotério provido de barreiras sanitárias (camundongos controle). A amostragem semestral aleatória consistiu em 5% da população das salas com mais de 100 animais cada. No exame clínico geral observaram-se distensão abdominal e diarréia nos camundongos e ratos dos biotérios convencionais, o que não foi constatado nos animais do biotério controle. Discreta alopecia e pêlos eriçados foram detectados em todos os animais das colônias estudadas. Infestações porMyobia musculi, Myocoptes musculinus, Radfordia affinis, Radfordia ensiferae Poliplax spinulosa foram identificadas nas colônias convencionais, em infestações múltiplas ou simples. Myobia musculiioi descrito pela primeira vez em ratos criados em biotérios. Infecções por endoparasitas Hymenolepis nana, Syphacia sp, Aspiculuris tetraptera, Trichomonas muris, Spironucleus muris, Giardia muris e Eimeria sp foram observadas nas colônias convencionais. Entretanto, através dos resultados da necrôpsia, na colônia controle de camundongos somente foram encontrados Syphacia sp e Aspiculuris tetraptera. A necrôpsia confirmou que o uso do método de anal-swab para detecção de ovos de Syphacia sp é preferível ao de Willis, e revelou o grau de infecção de protozoários intestinais. Syphacia sp, Hymenolepis nana e Aspiculuris tetraptera foram eficientemente detectados pelo método de Willis.
O funículo umbilical em bovinos azebuados
Authors: Ribeiro Antonio Augusto Coppi Maciel , Miglino Maria Angelica 
Published: September 2016

Nesta pesquisa, obtiveram-se dados sobre os aspectos microscópicos das estruturas vasculares que compõem o funículo umbilical de bovinos azebuados. Para a realização da pesquisa utilizaram-se funículos umbilicais provenientes de 30 fetos (13 machos e 17 fêmeas).Tanto as artérias quanto as veias apresentavam, na constituição das suas paredes, um repregueamento da túnica íntima que se mostrava bastante característico nas artérias. A túnica média das artérias e veias continha uma musculatura bem desenvolvida, fato que caracterizava esses vasos do tipo muscular. A luz dos vasos arteriais e venosos mostrava-se inicialmente (porção justa-fetal) quase obliterada e aumentava à medida que se aproximava da porção justa-placentária do funículo umbilical. A quantidade de fibras reticulares, elásticas e colágenas na parede dos vasos umbilicais foi comparada mediante técnicas histológicas específicas. Alguns aspectos inerentes ao dueto alantóide foram também observados.
Estudos hematológicos em Oreochromis niloticus (Linnaeus, 1758) (Cichlidae, Teleostei) - Parte I
Authors: Ueda Ivete Kotomi , Egami Mizue Imoto , Sassp Wilson da Silva , Matushima Eliana Reiko 
Published: September 2016

Avaliou-se o hemograma e a morfologia celular em amostras de sangue periférico obtidas de 10 peixes da espécie Oreochromis niloticus. Parte das mesmas foi utilizada para o estudo da hematimetria, isto é, contagem de eritrócitos (x = 2,35 x 106/mm3 ± 0,12), taxa de hemoglobina, (x = 7,04 g/dL ± 0,38), hematócrito (x = 27,85% ± 1,62), HCM (x = 30,31pg ± 1,64), VCM (x = 118,6p3 ± 3,53), CHCM (x = 25,78% ± 1,72), contagem global de leucócitos (x = 16,08 x 103/mm3 ± 1,28), contagem de trombócitos (x = 61,69 x 103/mm3 ± 5,96), sendo a avaliação da morfologia das células do sangue reallizada através do estudo de extensões sangüíneas submetidas à coloração de Wright. Os eritrócitos e trombócitos nucleados apresentaram-se elípticos; neutrófilos e eosinófilos esféricos de tamanho variável, com o núcleo também esférico, apresentaram citoplasmas abundantes, ligeiramente basófilo e altamente acidófilo, respectivamente. Não foram vistas as formas polimorfonucleares dessas células. Os basófilos apresentaram núcleo esférico e granuiações metacromáticas. Os monócitos e os linfócitos assemelharam-se morfologicamente aos dos mamíferos.
CORRIGENDUM
Authors: Baker Brad , Lawson R. Paul 
Published: September 2016

AbstractCorrections are made to the results, and interpretation thereof, presented in earlier work by Baker and Lawson. The main results regarding the improvement obtained using additional image parameters are unchanged. Secondary results regarding the applicability of subgroup parameterizations are corrected. Whereas it was found in the earlier work that very few subgroup parameterizations could be applied, it is now found that more subgroup parameterizations could be applied in situations in which crystal habits are sufficiently identifiable.
Expansion Pharyngoplasty by New Simple Suspension Sutures without Tonsillectomy
Authors: El‐Ahl Magdy Abdalla Sayed , El‐Anwar Mohammad Waheed 
Published: September 2016

The aim of this study was to introduce and assess a new minimally invasive expansion pharyngoplasty for obstructive sleep apnea (OSA) using bilateral new advancement sutures without tonsillectomy. Among 24 patients who had OSA with Friedman stage II or III and type I Fujita, bilateral sutures were performed to advance and stabilize palatopharyngeus and palatoglossus muscles anterolaterally. Mean apnea hypopnea index decreased significantly from 28.6 ± 4.2 preoperatively to 8.9 ± 4.9 postoperatively. The lowest oxygen saturation increased significantly from 79.25 ± 4.12 to 89.29 ± 4.12. Moreover, the visual analog score showed statistically significant reduction in the snoring intensity from a preoperative mean of 8.2 ± 1.4 to 2.1 ± 1.4 at 6 months postoperatively. Significant improvements were also documented in the Epworth Sleepiness Scale, as its mean decreased from 11.7 ± 2.9 preoperatively to 5.1 ± 2.2 postoperatively. In conclusion, the described new sutures could significantly correct OSA in patients with retropalatal obstruction and lateral pharyngeal walls collapse with easy applicability and no reported complication.
Optimizing Positioning for In‐Office Otology Procedures
Authors: Govil Nandini , DeMayo William M. , Hirsch Barry E. , McCall Andrew A. 
Published: September 2016

ObjectiveSurgeons often report musculoskeletal discomfort in relation to their practice, but few understand optimal ergonomic positioning. This study aims to determine which patient position—sitting versus supine—is ergonomically optimal for performing otologic procedures.Study DesignObservational study.SettingOutpatient otolaryngology clinic setting in a tertiary care facility.Subjects and MethodsWe observed 3 neurotologists performing a standardized simulated cerumen debridement procedure on volunteers in 2 positions: sitting and supine. The Rapid Upper Limb Assessment (RULA)—a validated tool that calculates stress placed on the upper limb during a task—was used to evaluate ergonomic positioning. Scores on this instrument range from 1 to 7, with a score of 1 to 2 indicating negligible risk of developing posture‐related injury. The risk of musculoskeletal disorders increases as the RULA score increases.ResultsIn nearly every trial, RULA scores were lower when the simulated patient was placed in the supine position. When examined as a group, the median RULA scores were 5 with the patient sitting and 3 with the patient in the supine position (P <. 0001). When the RULA scores of the 3 neurotologists were examined individually, each had a statistically significant decrease in score with the patient in the supine position.ConclusionThis study indicates that patient position may contribute to ergonomic stress placed on the otolaryngologist’s upper limb during in‐office otologic procedures. Otolaryngologists should consider performing otologic procedures with the patient in the supine position to decrease their own risk of developing upper‐limb musculoskeletal disorders.
Altered reward expectancy in individuals with recent methamphetamine dependence
Authors: Bischoff-Grethe Amanda , Connolly Colm G , Jordan Stephan J , Brown Gregory G , Paulus Martin P , Tapert Susan F , Heaton Robert K , Woods Steven P , Grant Igor ,   the TMARC Group
Published: September 2016

Background: Chronic methamphetamine use may lead to changes in reward-related function of the ventral striatum and caudate nucleus. Whether methamphetamine-dependent individuals show heightened reactivity to positively valenced stimuli (i.e. positive reinforcement mechanisms), or an exaggerated response to negatively valenced stimuli (i.e. driven by negative reinforcement mechanisms) remains unclear. This study investigated neural functioning of expectancy and receipt for gains and losses in adults with (METH+) and without (METH−) histories of methamphetamine dependence. Methods: Participants (17 METH+; 23 METH−) performed a probabilistic feedback expectancy task during blood-oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI). Participants were given visual cues probabilistically associated with monetary gain, loss, or neutral outcomes. General linear models examined the BOLD response to: (1) anticipation of gains and losses, and (2) gain and loss monetary outcomes. Results: METH+ had less BOLD response to loss anticipation than METH− in the ventral striatum and posterior caudate. METH+ also showed more BOLD response to loss outcomes than to gain outcomes in the anterior and posterior caudate, whereas METH− did not show differential responses to the valence of outcomes. Discussion: METH+ individuals showed attenuated neural response to anticipated gains and losses, but their response to loss outcomes was greater than to gain outcomes. A decreased response to loss anticipation, along with a greater response to loss outcomes, suggests an altered ability to evaluate future risks and benefits based upon prior experience, which may underlie suboptimal decision-making in METH+ individuals that increases the likelihood of risky behavior.
In vivo coronary artery plaque assessment with computed tomography angiography: is there an impact of iterative reconstruction on plaque volume and attenuation metrics?
Authors: Chen Zhongyi , Boldeanu Irina , Nepveu Simon , Durand Madeleine , Chin Anne S , Kauffmann Claude , Mansour Samer , Soulez Gilles , Tremblay Cécile , Chartrand-Lefebvre Carl 
Published: September 2016

Background Coronary computed tomography angiography (CTA) allows the evaluation of coronary plaque volume and low attenuation (lipid-rich) component, for plaque vulnerability assessment. Purpose To determine the effect of iterative reconstruction (IR) on coronary plaque volume and composition. Material and Methods Consecutive patients without coronary artery disease were prospectively enrolled for 256-slice CT. Images were reconstructed with both filtered back projection (FBP) and a hybrid IR algorithm (iDose4, Philips) levels 1, 3, 5, and 7. Coronary plaques were assessed according to predefined Hounsfield unit (HU) attenuation intervals, for total plaque and HU-interval volumes. Results Fifty-three patients (mean age, 53.6 years) were included. Noise was significantly decreased and signal-to-noise ratio (SNR) / contrast-to-noise (CNR) were both significantly improved at all IR levels in comparison to FBP. Plaque characterization was performed in 41 patients for a total of 125 plaques. Total plaque volume ranged from 104.4 ± 120.7 to 107.4 ± 128.9 mm3 and low attenuation plaque component from 40.5 ± 54.7 to 43.5 ± 58.9 mm3, with no statistically significant differences between all IR levels and FBP ( P = 0.786 and P ≥ 0.078, respectively). Conclusion IR improved image quality. Total and low attenuation plaque volumes were similar using either IR or FBP.
‘Impossible Groups that Flourish in Leaking Containers’—Challenging Group Analytic Theory?
Authors: Weinberg Haim 
Published: September 2016

All group therapy textbooks emphasize the importance of the setting for a successful outcome of the group. This setting includes clear boundaries of time and space, stable participation, and good leadership. All are seen as requirements to create a safe enough environment for participants to work on deep issues. In addition, for its normal development and progress, the group is expected to go through a stormy stage with disagreements and conflicts. My article will highlight groups that do not follow such ‘rules’. Surprisingly, the members are still able to work at deep levels, create intimate relationships, and benefit from the group. How is this possible? Should we change our theories? I link the success of these groups to the secure presence (Neeman-kantor, 2013, researching outdoor groups of wives of Israeli soldiers who suffer from war PTSD) of the leader and the imagined internalized group that the members create. Applying field theory explains how a multi-unconscious-fantasy makes these impossible groups possible.