bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary resveratrol improves immunity and antioxidant defense in ewes by regulating the rumen microbiome and metabolome across different reproductive stages
Authors: Li Xiongxiong , Sha Yuzhu , Li Shuyan , Wang Zhengwen , Yang Yanan , Jiao Ting , Zhao Shengguo 
Published: October 2024

IntroductionResveratrol (Res), a natural plant antitoxin polyphenol, is widely used in animal husbandry due to its antioxidant and anti-inflammatory properties, and current research has focused on humans, sows, and female mice. This study aimed to analyze the effects of dietary Res supplementation in ewes on antioxidant activity, immune responses, hormone levels, rumen microbiota and metabolites across various reproductive stages (estrus, pregnancy, and lactation).MethodsTwenty-four healthy ewe lambs (Hu sheep, 2 months old) with a similar body weight (BW) (mean: 21.79 ± 2.09 kg) were selected and randomly divided into two groups: the control group (Con) and the Res group (Res). The Res group received 10 mg/kg Res (based on BW) in addition to their basal diet.ResultsRes increased the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) in ewes at sexual maturity (p &lt; 0.05). Additionally, Res supplementation induced significant increases in serum glutathione peroxidase (GSH-Px), IgG, FSH, and LH levels during estrus (p &lt; 0.05); serum IgA, IgG and IgM during pregnancy and lactation (p &lt; 0.05); and serum LH, glucose, GSH-Px, and catalase (CAT) levels during lactation (p &lt; 0.05). Meanwhile, serum interleukin 1β (IL-1β) (p =0.005) and cholesterol levels (p = 0.041) during the lactation stage decreased following Res supplementation. Notably, colostrum IgA, IgG, and fat concentrations were significantly higher in the Res group than in the Con group (p &lt; 0.05). Moreover, Res altered the rumen microbiota in ewes. Specifically, the relative abundance of Prevotella (p &lt; 0.05) during pregnancy and Rikenellaceae_RC9_gut_group (p &lt; 0.001) during lactation were significantly increased in ewes under Res treatment. The abundance of Rikenellaceae_RC9_gut_group was positively correlated with the levels of Ig A, Ig M, E2, FSH, LH, GSH-PX, and CAT. Additionally, Res altered the activity of metabolic pathways such as progesterone-mediated oocyte maturation, the estrogen signaling pathway, ovarian steroidogenesis, and the AMPK signaling pathway, and the levels of AICAR and 2-hydroxyestradiol metabolites, both during pregnancy and lactation.DiscussionThere findings show that Res can improve health, antioxidant status, and immune activity throughout the reproductive cycle in ewes by regulating rumen microorganisms and metabolites.
Camphor alters occipital electrocorticographic patterns during sleep deprivation in Wistar rats
Authors: Gurgel do Amaral Anthony Lucas , Barbosa Gabriela Brito , Farias dos Santos Murilo , Vasconcelos de Souza Luana , Gonçalves dos Santos Rodrigo , Câmara Tays Mata , de Sousa Reis Thaysa , Pacheco Hartcopff Priscille Fidelis , Eiró-Quirino Luciana , Araújo da Paz Clarissa , Bastos de Araújo Daniella , Favacho Lopes Dielly Catrina , Saito Miki Taketomi , Hamoy Moisés 
Published: October 2024

IntroductionSleep disorders are common in the general population, necessitating the search for new strategies to address this public health challenge. The study aims to describe the electrocorticographic and behavioral changes in sleep deprived Wistar rats exposed to varying doses of camphor, to assess its effects on sleep and its potential as a sleep-inducing drug.Materials and MethodsFor the electrocorticographic evaluation, seventy-two rats were randomly assigned to distinct groups: a control group, a sleep-deprived group, three sleep-deprived groups receiving 10, 20, and 30 mg/kg i.p. of camphor respectively, and three groups that received these doses without sleep deprivation. For the behavioral analysis, twenty-seven rats were divided into three groups, each receiving the same doses as the previous test.Results and DiscussionOur results showed that there was a decrease in the frequency of brain oscillatory patterns when camphor was administered at 10 mg/kg i.p. whereas there was a dose-dependent increase in the spectral power and distribution following the administration of 20 and 30 mg/kg i.p., with the emergence of Delta, Theta, Alpha, and Beta waves. As for the behavioral analysis, it was demonstrated that testicular relaxation, decreased motility, and light sleep induction also occurred in a dose-dependent manner. Thus, we conclude that camphor administration intensifies occipital electrocorticographic patterns in sleep-deprived rats, and its electrocorticographic and behavioral analysis could indicate a potential as a supporting agent in the insomnia treatment.
Association between vitamin B6 levels and rheumatoid arthritis: a two-sample Mendelian randomization study
Authors: Liu Yanglin , Wang Xukai , You Min , Zheng Meiling , Yu Miao , Leng Xiangyang 
Published: October 2024

BackgroundMicronutrients play a crucial role in rheumatoid arthritis (RA). Changes in micronutrient levels in RA patients can lead to the worsening of their condition. Though significant correlations between RA and micronutrients have been found in earlier observational studies, their underlying causal relationship is still unknown. This study aimed to elucidate the causal genetic relationships between 15 micronutrients (copper, zinc, magnesium, vitamins A, C, E, D, B6, B12, folate, carotene, iron, selenium, calcium, potassium) and RA.MethodThe exposure factors and outcome data used in the two-sample Mendelian randomization (MR) were derived from publicly available summary statistics data of European populations. The GWAS data for exposure factors were obtained from the OpenGWAS database. For the outcome data of RA, we utilized data from the FinnGen database. We used the MR principle to remove confounding factors and conducted MR analyses using five methods: inverse variance weighted (IVW), MR Egger, weighted median, simple mode, and weighted mode, with IVW as the primary method. Then, we identified micronutrients related to RA and performed MR analyses on these elements, including heterogeneity analysis and pleiotropy analysis such as MR-Egger intercept, MR-PRESSO method, and “leave-one-out” analysis. Finally, we conducted multivariable MR analyses and performed sensitivity analyses again.ResultsThe IVW analysis revealed a relationship between vitamin B6 and RA (p: 0.029, OR: 1.766, and 95% CI: 1.062–2.938). Sensitivity analysis confirmed the validity and reliability of this result.ConclusionThis study revealed a causal relationship between vitamin B6 and RA, with vitamin B6 being identified as a risk factor for RA. This finding could contribute to the diagnosis and supplementary treatment of RA patients, providing a reference for subsequent basic research and developing new drugs.
Modeling and empirical evidence of the impact of implementation of sugar sweetened-beverages tax to reduce non-communicable diseases prevalence: a systematic review
Authors: Firdaus Safira , Andarwulan Nuri , Hariyadi Purwiyatno 
Published: October 2024

IntroductionThe surge in non-communicable diseases (NCDs) has been linked to excessive sugar-sweetened beverage (SSB) consumption. In response, the World Health Organization advocates for SSB taxes as a preventive measure. This study conducts a systematic literature review, encompassing simulation modeling and empirical evidence, to evaluate the effectiveness of SSB taxes in diminishing NCD prevalence.MethodA systematic search from August 2002 to August 2022, utilizing databases like ScienceDirect, PubMed, Google Scholar, Wiley Online Library, Springer, and ResearchGate, identified 29 relevant articles worldwide following PRISMA 2020. The Critical Appraisal Skill Programme (CASP) 2018 tool was employed for economic evaluationResultAmong the selected articles, 22 utilized simulation models in group of countries such as South Africa, the US, the UK, Asia (Philippines, India, Indonesia, Thailand), Australia, and Europe (Germany), while seven were based on US and UK evidence. Simulation modeling consistently demonstrated that SSB taxes significantly reduced NCD incidence, prevalence, and mortality, also bolstering government revenue. Tax rates in simulations ranged from 10 to 25%. However, empirical evidence indicated a limited impact, primarily due to low tax rates. Notably, a UK-specific tax led to a 2.7% reduction in SSB sugar purchases and 40.2% higher purchases of no-levy drinks.DiscussionThe findings suggest that while simulation models demonstrate the potential effectiveness of SSB taxes in reducing NCDs, empirical evidence reveals there is no significant effect of the SSB tax, Based on the study conducted in this study, the SSB tax is not effective in reducing the prevalence of NCDs due to consumer preferences that have not changed. , likely due to the implementation of lower tax rates and failure to fulfill the assumption of subtitution product, physical activity, and so on. The study highlights that SSB tax is not effective in reducing the prevalence of NCDs due to consumer preference that have not change. Multi-actions are needed to support the sustainability of the implementation of the SSB tax, including education and promotion of healthy lifestyles and encouragement to reformulate SSB products by industry.
ED50 of ciprofol combined with different doses of remifentanil during upper gastrointestinal endoscopy in school-aged children: a prospective dose-finding study using an up-and-down sequential allocation method
Authors: Zhang Xu , Zhang Ning , Song Haicheng , Ren Yueyi 
Published: October 2024

ObjectiveThis study aimed to determine the 50% effective dose (ED50) of ciprofol when combined with different doses of remifentanil for upper gastrointestinal endoscopy of school-age children and to evaluate its safety.MethodsThis study involved school-aged children who were scheduled to undergo upper gastrointestinal endoscopy under deep sedation. The children were randomly assigned to two groups: remifentanil 0.3 μg/kg (R0.3) and remifentanil 0.5 μg/kg (R0.5). Anesthesia was induced with remifentanil, followed by ciprofol. The dose of ciprofol for each patient was determined using the Dixon up-down sequential method. If the MOAA/S score was ≤1 and the child did not exhibit significant movement or coughing during the endoscopy process, sedation was considered successful. The first patient in each group received 0.5 mg/kg ciprofol. The dose of ciprofol was adjusted by 0.05 mg/kg based on the response of the previous patient. The primary outcome was the ED50 of the ciprofol-induction dose. The total ciprofol doses, onset times, awakening times, and adverse reactions were recorded.Results1) The Dixon method was used to collect crossovers data from each group, and the ED50 values of the R0.3 and R0.5 groups were calculated to be 0.390 mg/kg (95% CI 0.356–0.424 mg/kg) and 0.332 mg/kg (95% CI 0.291–0.374 mg/kg), respectively. The ED50 of ciprofol in the R0.3 group was significantly higher than that in the R0.5 group (p &lt; 0.05). 2) The onset time and recovery time of the R0.5 group were shorter than those of the R0.3 group (p &lt; 0.05). When the two groups were compared, the total dose of ciprofol in the R0.5 group decreased (p &lt; 0.05). 3) Compared with the R0.3 group, the incidence of respiratory depression during induction in the R0.5 group increased (p &lt; 0.05).ConclusionThis study explored the ED50 of ciprofol combined with different doses of remifentanil for successful sedation in upper gastrointestinal examinations in school-aged children. Compared to the use of remifentanil 0.3 μg/kg, the combination of ciprofol with remifentanil 0.5 μg/kg significantly reduced the ED50 required to prevent body movement or cough during endoscope insertion but increased the incidence of respiratory depression.
HLA-B allele frequencies and implications for pharmacogenetics in the Kuwaiti population
Authors: Dashti Mohammed , Malik Md Zubbair , Al-Matrouk Abdullah , Bhatti Saeeda , Nizam Rasheeba , Jacob Sindhu , Al-Mulla Fahd , Thanaraj Thangavel Alphonse 
Published: October 2024

Objective: This study explores the frequency of human leukocyte antigen (HLA) genes, particularly HLA-B alleles, within the Kuwaiti population. We aim to identify alleles with known associations to adverse drug reactions (ADRs) based on existing literature. We focus on the HLA-B gene due to its well-documented associations with severe cutaneous adverse reactions and the extensive pharmacogenetic research supporting its clinical relevance.MethodsWe utilized the HLA-HD tool to extract, annotate, and analyse HLA-B alleles from the exome data of 561 Kuwaiti individuals, sequenced on the Illumina HiSeq platform. HLA typing was conducted using the HLA-HD tool with a reference panel from the IPD-IMGT/HLA database. The major HLA-B pharmacogenetic markers were obtained from the HLA Adverse Drug Reaction Database, focusing on alleles with significant ADR associations in published literature.ResultsThe distribution of HLA-B alleles in the Kuwaiti population revealed that the most frequent alleles were HLA-B*50:01 (10.52%), HLA-B*51:01 (9.89%), HLA-B*08:01 (6.06%), HLA-B*52:01 (4.55%), HLA-B*18:01 (3.92%), and HLA-B*41:01 (3.65%). Notably, alleles HLA-B*13:01, HLA-B*13:02, HLA-B*15:02, HLA-B*15:13, HLA-B*35:02, HLA-B*35:05, HLA-B*38:01, HLA-B*40:02, HLA-B*44:03, HLA-B*51:01, HLA-B*57:01 and HLA-B*58:01 were identified with known associations to various ADRs. For example, HLA-B*51:01 was associated with clindamycin, phenobarbital, and phenytoin, and was found in 18% of individuals.ConclusionOur study enriches the regional genetic landscape by delineating HLA-B allele variations within Kuwait and across the Arabian Peninsula. This genetic insight, along with the identification of markers previously linked to drug hypersensitivity, provides a foundation for future pharmacogenetic research and potential personalized medicine strategies in the region.
Research on mid-infrared quantum cascade lasers spectral beam combining
Authors: Fan Xinmin , Zhang Jianxin , Wang Yan , Li Sensen , Wang Chunyan , Wu Yuantai , Zhang Lujun , Huang Xiaodong , Li Shun , Zhang Yu 
Published: October 2024

Quantum cascade lasers (QCLs) in the mid-infrared (MIR) hold significant potential for widespread applications in both military and civilian contexts. However, the utility of present single-chip QCLs is hampered by issues such as low output power and subpar beam quality. This study addresses these limitations by employing spectral beam combining (SBC) based on a diffraction grating, with an aim to enhance both power and beam quality of MIR QCLs. Coaxial power synthesis of three single-chip QCLs (around 4.75 μm) is achieved experimentally, importantly, the beam quality did not decrease after combining, essentially maintaining the same quality as before combining. It is proposed that there are four main factors affecting the combining efficiency, namely operating optical power (or driving current) of the chips, individual differences in QCL chips, diffraction grating, and reflectivity of feedback mirror, and their effects on the combining efficiency are discussed separately. This study confirms that SBC is an effective way to obtain high-power and high-beam quality MIR QCL sources, and lays a research foundation for more beam spectral combining.
Investigating mood-modification, withdrawal, and sensitization in compulsive sexual behaviour
Authors: Sassover Eli , Kushnir Talma , Weinstein Aviv M. 
Published: October 2024

Background and aimsCompulsive Sexual Behaviour (CSB), defined as a persistent failure to control repetitive sexual impulses, has been discussed as a pathological phenomenon for centuries. Various terms, such as excessive sexual behaviour, hyper-sexuality, compulsive sexual behaviour disorder (CSBD), or sexual addiction (SA), have been used to describe it, contributing to ongoing debates about its theoretical framework. The following three studies aim to empirically assess whether CSB exhibits key elements of behavioural addiction (mood-modification, sensitization/tolerance, and withdrawal).MethodThree studies, involving participants with and without CSB, were conducted. The mood-modification hypothesis was tested by exposing participants to short films inducing positive, negative, and emotionally neutral moods, followed by an evaluation of their craving for pornography. To test the sensitization hypothesis, participants viewed short films with varying levels of explicit sexual stimuli, and their level of actual wanting and liking were assessed through self-reports. For the withdrawal hypothesis, participants underwent a 10-day sexual abstinence, with self-reports of various symptoms, collected on pre-intervention, 3rd, 7th, and 10th days.ResultsContrary to previous studies of addiction, CSB participants didn’t show increased craving to mood induction and negative mood actually decreased craving for pornography. Secondly, they showed wanting to explicit sexual stimuli although it was not increased with explicitness. Finally, they demonstrated reduced withdrawal symptom during abstinence.ConclusionThe results of this study provide conflicting results concerning the model of behavioural addiction. There is supporting evidence for wanting in response to explicit pornography stimuli although it was not associated with increased explicitness. There is also evidence for reduced withdrawal during abstinence. Finally, there was no evidence that mood modification increases craving for pornography, negative mood actually decreased craving. Further research is needed to test the various models of CSB.
Risk assessment of human-to-human transmission of severe fever with thrombocytopenia syndrome virus based on 10-year clustered analysis
Authors: Zhang Nannan , Mu Xiaodong , Liu Jingyu , Liu Tao 
Published: October 2024

BackgroundSevere fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease, which was first reported in 2009 in China. Previous studies have rarely quantitatively assessed the transmission and fatal risk of SFTS clusters.MethodsEpidemiological information regarding SFTS clusters in Yantai city of Shandong province during 2013-2022 was obtained from the National Public Health Emergency Event Surveillance System (PHEESS) for Disease Control and Prevention information system. The secondary attack rate (SAR) and relative risk (RR) were used to assess the risk of human-to-human transmission of SFTS.ResultsA total of 20 SFTS clusters involving 51 laboratory-confirmed patients were reported between 2013 and 2022 in Yantai city, Shandong province. Most of the clusters occurred from May to October, and the patients were mainly distributed in four counties. Contact with blood or other fluids [RR = 14.06, 95% confidence interval (CI) = 3.29–70.65, p &lt; 0.001] and using no personal protection equipment (PPE) [11.63% (10/86) vs. 2.22% (2/90), RR = 5.74, 95% CI = 1.17–55.44, p = 0.013] were significantly related with an increased risk of SFTS virus (SFTSV) transmission.ConclusionOur study may provide direct guidance on health education and behavioral interventions for the accompanying relatives and personnel of SFTS patients, both during their hospital stay and upon returning home after discharge.
Recommendations for long-term follow-up care of secondary health conditions in spinal cord injury/disorder: a systematic review
Authors: Eriks-Hoogland Inge , Jordan Xavier , Baumberger Michael , Seijas Vanessa , Huber Burkhart , Michel Franz , Thietje Roland , Müller Lorena 
Published: October 2024

ObjectivesThe purpose of this systematic review is to provide an overview of published follow-up care programs of primary and secondary health conditions (SHCs) in spinal cord injury/disorder (SCI/D) and spina bifida and describe recommendations on content, frequency, setting of follow-up care programs for persons with SCI/D and spina bifida.MethodsAccording to the sequence of procedures of the AWMF (Association of the Scientific Medical Societies in Germany) a systematic literature search was performed (in PubMed, Cochrane Library and nine additional databases for guidelines) between 5 September 2019 and 22 September 2019. Publications (Jan. 2008–Dec. 2018) and guidelines (up to 2018) published in English or German and describing an evidence-based follow-up care program for persons with SCI/D or spina bifida were included.ResultsThe systematic literature search found 1973 publications in PubMed and Cochrane Library, resulting in 19 papers for SCI/D and 6 for Spina bifida. Additionally, we included 34 guidelines developed by reputable committees or medical associations. All eligible guidelines, and publications, were rated and classified according to the guidance of AWMF. Of the retrieved publications, and guidelines, level of evidence of follow-up care programs was mostly based on informal procedures and expert opinion or formally consent based expert opinion. None of the guidelines, or publications described an evidence based comprehensive clinical practice guideline (CPG) for follow-up care for people with SCI/D or spina bifida.ConclusionBased on the comprehensive and extensive literature research conducted, regular (annual) follow-up care appointments at specialized SCI clinics are recommended. There is a notable absence of a comprehensive CPG covering all relevant health conditions for long-term follow-up in SCI/D or spina bifida. In order to provide persons with SCI/D with up-to-date and best possible medical and rehabilitative care, a CPG for follow-up care is urgently needed. In response to this gap, the German-speaking Medical Society of Paraplegia (DMGP) has commissioned its members to establish a guideline for follow-up care for individuals with SCI/D. The current review serves as an evidence-based framework for the development of this guideline.
Training, environmental and nutritional practices in indoor cycling: an explorative cross-sectional questionnaire analysis
Authors: Peeters W. M. , Coussens A. H. , Spears I. , Jeffries O. 
Published: October 2024

IntroductionIndoor cycling at home has grown rapidly in recent years facilitated by advances in technology and gamification. However, there is limited data on individual's training practices when cycling indoors.MethodsUsing a single-time point, cross-sectional questionnaire, we gathered information on equipment, environmental considerations, training practices and nutrition during indoor cycling.ResultsFollowing 492 responses, external variables (weather; 88.4%, lack of daylight; 56.3%), time efficiency (81.9%) and general fitness (70.9%) were most frequently cited as reasons to engage in indoor cycling. “Smart” turbo trainers linked to a mixed-reality cycling software were most frequently reported in equipment set-up. 78% of participants attempted to control temperature with 96% of these participants using at least 1 fan to control airflow. The volume of indoor training differed between seasons (winter: 6h10 ± 3 h 30, summer 2h52 ± 2h57, p &lt; 0.001), and structured (61.9%) and unstructured work-outs (64.7%) were completed more than once a week with fewer participants engaging in competitive/racing events (20.9%). 98% percent of participants consumed fluids with an average fluid intake of 0.74 ± 0.28 L/h. Dependent on type of work-out, participants reported less planning of carbohydrate and protein intake during short-duration work-outs (∼40%–60%) relative to longer-duration work-outs (∼56%–80%). Caffeine use was the most frequently reported ergogenic aid.ConclusionTogether we report indoor cycling practices with respect to training equipment, considerations of environmental and nutritional strategies and training habits. Our findings could be used to support the development of future research and indoor training guidelines.