bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential habitat suitability analysis under climate change for the native grass, Kengyilia thoroldiana, on the Qinghai-Tibet Plateau
Authors: Zhang Hairong , He Miaohua , Shi Jianjun , Ma Yuan , Ou Weiyou , Xing Yunfei , Liu Qingqing , Xu Gongfang , Lv LiangYu , Cai Zongcheng 
Published: September 2024

Abstract

Kengyilia thoroldiana is a dominant species in the desertification area of the Qinghai-Tibet Plateau. In this study, based on 88 sample points of Kengyilia thoroldiana, the Maximum Entropy (MaxEnt) method was used to analyze the current dominant factors of the distribution area of Kengyilia thoroldiana and predict its potential distribution. The results show that the training and test data area under the curve (AUC) were 0.934 ± 0.004 and 0.944 ± 0.043, respectively, which indicated the reliability of the predicted results. Based on climatic variables and the results of the “Jackknife” method, The results showed that temperature is the main cause of Kengyilia thoroldiana distribution. By simulating the potential distribution of Kengyilia thoroldiana, the highly suitable areas were mainly located in the west, south, and southeast of Qinghai, southwest Gansu, and eastern Tibet. In future climate scenarios, the total suitable area for Kengyilia thoroldiana showed an expanding trend. According to the Sustainable Development (SSP126) scenario, the highly suitable areas could increase by 4.72% from 2021 to 2040 compared with the current climate scenario, and the highly suitable areas could increase by 12.71% from 2041 to 2060. An increase in the suitable areas of Kengyilia thoroldiana is essential for the ecological restoration of degraded grasslands.
Development of a skeletal muscle sheet with direct reprogramming-induced myoblasts on a nanogel-cross-linked porous freeze-dried gel scaffold in a mouse gastroschisis model
Authors: Nagano Shinta , Fumino Shigehisa , Kishida Tsunao , Wakao Junko , Hirohata Yoshiaki , Takayama Shohei , Kim Kiyokazu , Akiyoshi Kazunari , Mazda Osam , Tajiri Tatsuro , Ono Shigeru 
Published: September 2024

Abstract

Purpose: In this study, we attempted to create skeletal muscle sheets made of Directly converted myoblasts (dMBs) with a nanogel scaffold on a biosheet using a mouse gastroschisis model.
Methods: dMBs were prepared by the co-transfection of MYOD1 and MYCL into human fibroblasts. Silicon tubes were implanted under the skin of NOG/SCID mice, and biosheets were formed. The nanogel was a nanoscale hydrogel based on cholesterol-modified pullulan, and a NanoClip-FD gel was prepared by freeze-drying the nanogel. 7-mm in length were created in the abdominal wall of NOG/SCID mice as a mouse gastroschisis model. Matrigel or NanoCliP-FD gel seeded with dMBs was placed on the biosheet and implanted on the model mice.
Results: Fourteen days after surgery, dMBs with matrigel showed a small amount of coarse aggregations of muscle-like cells. In contrast, dMBs with NanoCliP-FD gel showed multinucleated muscle-like cells, which were expressed as Desmin and Myogenin by fluorescent immunostaining.
Conclusion:Nanogels have a porous structure and are useful as scaffolds for tissue regeneration by supplying oxygen and nutrients supply to the cells. Combining dMBs and nanogels on the biosheets resulted in the differentiation and engraftment of skeletal muscle, suggesting the possibility of developing skeletal muscle sheets derived from autologous cells and tissues.
Characteristics of eclampsia not preceded by hypertensive disorders of pregnancy: a case control study
Authors: Sato Maki , Iino Kaori , Ozaki Takashi , Hashimoto Tetsuji , Osawa Yuki , Hashimoto Rikako , Tando Tomoe , Takeda Aisa , Takahashi Sota , Taniguchi Ryosuke , Oishi Maika , Ito Asami , Tanaka Kanji , Yokoyama Yoshihito 
Published: September 2024

Abstract

Background Eclampsia, a severe complication of pregnancy characterized by seizures, has traditionally been associated with hypertensive disorders of pregnancy (HDP). However, a significant proportion of eclampsia cases occur without a prior diagnosis of HDP, suggesting the existence of alternative pathophysiological mechanisms. This study aimed to explore the characteristics of eclampsia not preceded by HDP and to offer insights into its underlying mechanisms and potential risk factors.Methods We conducted a retrospective analysis of eclampsia in 31 singleton pregnancies diagnosed at nine Japanese hospitals with perinatal and maternal centers between May 2007 and March 2023. Data on background factors, blood pressure measurements, laboratory test results, and imaging findings were collected and analyzed. Patients were classified into two groups: eclampsia preceded by HDP and eclampsia not preceded by HDP, and comparisons were made regarding background factors, perinatal outcomes, and test results were compared.Results Of the 30 cases of eclampsia, 17 (57%) were not preceded by HDP, while the remaining cases had been diagnosed with HDP prior to the onset of eclampsia. In the eclampsia not preceded by HDP group, compared to the eclampsia preceded by HDP group, the BMI was lower, the gestational week at delivery was earlier, and the proportion of male neonates was higher (P < 0.05). When comparing the onset periods among the three groups—antepartum, intrapartum, and postpartum—the eclampsia not preceded by HDP group exhibited the highest number of cases during the intrapartum period, with 9 cases (52.9%). Conversely, the eclampsia preceded by HDP group showed the highest number of cases during the postpartum period, with 8 cases (61.5%). Furthermore, in the Eclampsia not preceded by HDP group, the white blood cell count was significantly higher immediately after onset (P < 0.05).Conclusions Eclampsia not preceded by HDP may involve inflammatory and immune responses triggered by labor, unlike typical eclampsia, which is associated with a rapid increase in blood pressure. Recognizing atypical eclampsia as a separate entity may aid in developing more effective prediction, prevention, and treatment strategies for this life-threatening condition.
AKAP8 regulates R-loop balance and promotes growth of lung carcinoma cell
Authors: Wang Xu , Liu Liang 
Published: September 2024

Abstract

Background RNA:DNA hybrid structure known as R-loop, which forms during transcription plays a pivotal roles in transcriptional regulation. Dysregulation of R-loop dynamics disrupt normal DNA replication or RNA transcription, potentially leading to disturbances of cell metabolism, abnormal cell proliferation and disease progression.Methods Interactome data of nucleic AKAPs and R-loop were collected and analyzed to nominate the candidate of AKAP8 (A-kinase-anchoring protein 8) as R-loop binding protein. The interaction of AKAP8 and R-loop were confirmed by co-immunoprecipitation and immunofluorescence. R-loop resolution protein DDX5 were identified to interact with AKAP8 and its nucleic abundance was estimated. AKAP8 knock down cell lines were constructed. The mRNA profile and differential expressed genes of were analyzed. Downstream target gene UCP2 was confirmed upregulate by AKAP8 and R-loop level of UCP2 promoter was estimated. Cell growth and migration of lung carcinoma cell line with depletion of AKAP8 or not were also investigated by EdU, colony formation and wound healing essay. Expression score of AKAP8 comparing lung cancer tissue with normal tissue, and correlation between survival possibility of lung cancer patients and expression level of AKAP8, were also investigated.Results This study identified that AKAP8 interacted with R-loop structure within cells. Depletion of AKAP8 resulted in perturbation of genomic R-loop balance and gene transcription. Evidences was shown that AKAP8 interacted with R-loop resolution protein DDX5 and regulated chromatin associated DDX5 level. Furthermore, AKAP8 was found to enhance transcription uncoupling protein UCP2 as well as alleviate R-loop level of UCP2 promoter, and promoted cell growth and migration of lung carcinoma cell. The lower survival possibility was found in lung cancer patients with high level AKAP8 expression.Conclusions This study elucidates novel roles of AKAP8 in modulating R-loop balance by cooperation of DDX5 and AKAP8 is as one of the motivators for lung carcinoma cell growth contributed by mitochondrial metabolism. This insight may offer prognostic significance for patients with lung adenocarcinoma exhibiting higher AKAP8 expression.
Bi-directional causal association between 17 neurosurgical diseases and three emotional disorders: perspective from Mendelian randomization analysis
Authors: Chen Haonan , Zhang Renhao , Wen Xinjie , Shao Dongqi , Fu Qiang , Yin Shichao , lv Yifan , Sun Tao 
Published: September 2024

Abstract

Background
Although numerous studies have reported correlations between emotional disorders and neurosurgical conditions, their causal relationships is not convincing. Therefore, we seek to comprehensively investigate the connection between the two using bidirectional Mendelian randomization studies.
Methods The GWAS aggregated data encompassed 17 types of neurosurgical diseases (including cerebrovascular diseases, functional disorders, central nervous system neoplasms, spinal and spinal cord diseases, and other brain conditions) and 3 emotional disorders (anxiety, mania, and depression), sourced from IEU and FINNGEN. The primary analysis method applied was inverse variance-weighted (IVW) analysis, supplemented by MR-Egger and weighted median methods to ensure robust estimates. A series of sensitivity analyses, including Cochran’s Q test, MR-Egger regression, and leave-one-out analysis, were conducted to detect pleiotropy or heterogeneity.
Results IVW estimates indicated that trigeminal neuralgia significantly associated with the risk of mania (p=0.002, odds ratio [OR]=1.008, 95 % confidence interval [CI] = 1.003 to 1.014), a higher genetic predisposition to congenital malformations of nervous system may reduce the development of depression (p=0.002, OR= 0.996; 95 %CI = 0.992 to 0.998) and the causal effect of depression on transient ischemic attack (IVW, P=0.004, odds ratio (p=0.004, OR = 4.141; 95 %CI = 1.560 to 10.988). The results of comprehensive sensitivity analyses were consistent with the main causality estimate. No pleiotropy and heterogeneity were detected in our MR study.
Conclusions Our large-scaled MR analysis indicated that trigeminal neuralgia and congenital malformations of the nervous system predispose patients to emotional disorders, while depression, in particular, increases vulnerability to transient ischemic stroke.
Case Report: Thrombectomy for left internal carotid artery occlusion with ipsilateral dual accessory middle cerebral arteries
Authors: Tega Jota , Horio Yoshinobu , Suzuki Koichiro , Oka Yuta , Takemoto Koichiro , Abe Hiroshi 
Published: September 2024

Abstract

Accessory middle cerebral arteries (AMCAs) are found in 0.3-4.0% of cases, and ipsilateral dual AMCAs are rare. A seventy-three-year-old man presented with right hemiplegia and total aphasia. Magnetic resonance angiography showed left carotid artery occlusion. We infused him with tissue plasminogen activator. Digital subtraction angiogram showed revascularization of the left internal carotid artery. However, the left proximal anterior cerebral artery was occluded. We performed mechanical thrombectomy and achieved partial reperfusion. CT angiography on the tenth day showed ipsilateral dual AMCAs. Due to middle cerebral artery anomalies, we performed mechanical thrombectomy using contact aspiration which is safer than other techniques.
Healthcare Providers and the Lingering Influence of the COVID-19 Pandemic
Authors: Ness Michelle , DiFusco Leigh Ann 
Published: September 2024

Abstract

Purpose
To gain a better understanding of the experiences of healthcare providers caring for adult patients during the second year of the COVID-19 pandemic.
Methods
A descriptive, qualitative design was conducted using semi-structured interview questions. Eleven participants were recruited from a sample of 28 healthcare providers to participate in video interviews. Thematic analysis was used to analyze data.
Results
Healthcare providers experienced continued distress related to concerns about the long-term effects on themselves, their families, and society. Three themes emerged from the data: (1) exhausted and emotionally numb; (2) vaccinated versus the unvaccinated; and (3) stressors and misguided supports.
Conclusion
The lingering COVID-19 pandemic led to continued distress among healthcare providers. Themes highlight the challenges faced by healthcare providers as they continued to care for patients during the lingering pandemic. Leadership must continue to prioritize support for healthcare providers for the duration of healthcare disasters to promote quality of life, reduce burnout, and increase job satisfaction. Findings from this study can inform the development of much needed institutional support for front line healthcare providers during times of crisis.
The Norwood with Blalock-Taussig-Thomas Shunt – Worth a Second Look?
Authors: Gadiraju Manasa , Chauhan Dhaval , Schray Alexandra , O'brien James E. , Bedzra Edo 
Published: September 2024

Abstract

Background
Shunt repair for the Norwood procedure with either the right ventricle to pulmonary artery shunt (RVPAS) or the modified Blalock-Taussig-Thomas shunt (BTTS) varies by institution and surgeon preference. Shunt choice has been informed by landmark trials including the Single Ventricle Reconstruction trial and modern outcomes data may engender future complementary studies.
Methods
We conducted a retrospective analysis of all patients who underwent the Norwood procedure from 2014–2022 at a single center to compare outcomes by shunt type. The primary outcome measure was freedom from death or transplant. Secondary outcome measures included hospital length of stay, complications, and unplanned interventions.
Results
93 patients underwent the Norwood procedure at a median age of 7 days (IQR 5,9) and 39 weeks gestation (IQR 38,39). 67.7% had hypoplastic left heart syndrome. 39 patients received a BTTS compared to 54 RVPAS. There was no difference in operative mortality (BTTS 12.8%, RVPAS 9.3%, p = 0.58), death or transplant at 1 year (BTTS 15.4%, RVPAS 7.4%, p = 0.31), or between 1 and 3 years (BTTS 0%, RVPAS 5.6%, p = 0.26). There was a significantly higher rate of pulmonary arterial stenting in the RVPAS group (BTTS 1.21/100 patient-years, RVPAS 15.68/100 patient-years, p = 0.01).
Conclusions
Similar short- and medium-term survival was seen in BTTS and RVPAS groups with fewer pulmonary artery interventions for BTTS, though our study is underpowered to suggest superior freedom from interventions. These results may serve as a hypothesis-generating study to revisit the SVR trial with a modern cohort in the setting of improved surgical technique and perioperative management.
Surgical Techniques and Prognostic Nomogram for Patients with Supravalvular Aortic Stenosis
Authors: Sun Yuekun , Lv Lizhi , Zhi Aihua , Zhang Simeng , Wang Cheng , Wang Qiang 
Published: September 2024

Abstract

Background
An effective prognostic nomogram to predict the prognosis of patients with supravalvular aortic stenosis (SVAS) is lacking. This study sought to report the outcomes of different surgical techniques, identify the risk factors for postoperative re-operation or restenosis, and establish a prognostic nomogram.
Methods
This multi-centred retrospective cohort study included consecutive patients with SVAS who underwent surgery between 2002 and 2020. Patients underwent McGoon, Doty, and other repairs. The primary outcome was re-operation or restenosis at follow-up. The nomogram based on Cox regression and the Kaplan–Meier method was used to identify the risk factors for the primary outcome. The predictive accuracy was determined using the concordance index (C-index) and calibration curve. The results were validated using the bootstrap resampling method.
Results
Of the 291 patients with SVAS, 143 (49.1%) used McGoon repairs, 118 (40.5%) used Doty repairs, and 30 (10.3%) used other repairs. The median age at operation was 4.9 years (inter-quartile range (IQR) 2.3–9.9). After a median follow-up of 24 months (IQR 6.0–54.0), no difference in re-operation or restenosis was found between McGoon and Doty repairs. Age, gender, SVAS type, pulmonary artery stenosis, aortic valve stenosis, sinotubular junction z-score, and gradient were considered independent risk factors by least absolute shrinkage and selection operator regression and were included in the nomogram. The C-index of the nomogram was 0.71 (95% CI, 0.61 to 0.81). The calibration curve for the probability of re-operation or restenosis showed good agreement between prediction by nomogram and actual observation.
Conclusions
McGoon and Doty repairs showed no difference in re-operation and restenosis risk. The nomogram accurately predicted re-operation or restenosis in patients with SVAS after surgery. Trial Registration:
Influence of supporting teeth quantity of surgical guide on the accuracy of the immediate implant in the maxillary central incisor: An in vitro study
Authors: Tran Truong , Nguyen Meo , Nguyen Khanh , Nguyen Nga , Huynh Nam 
Published: September 2024

Abstract

Introduction: Static computer-assisted implant surgery (sCAIS) aims to enhance precision by guiding the drill during the procedure. The quantity and morphology of supporting teeth influence the accuracy of sCAIS.
Aims: To investigate how the number of supporting teeth affects the accuracy of immediate implant surgery in the maxillary central incisor.
Methods: Twenty-eight upper jaw models, simulating immediate post-extraction sockets of tooth 11, were divided into two groups based on the number of supporting teeth (four or six). Cone beam computed tomography and intraoral scans determined the implant positions, guiding the fabrication of surgical guides and evaluating discrepancies in the planned and actual implant placements, particularly in terms of three-dimensional at the implant platform and apex and angular deviations.
Results: The group with six teeth support had significant differences in angular deviation (3.59 ± 0.97 and 4.63 ± 0.71) and implant apex deviation (1.40 ± 0.27 and 2.08 ± 0.21) values compared to the group with four teeth support. Although other discrepancy variables in the six teeth-supported group exhibited lower values, they did not show statistically significant differences compared to the four teeth-supported group.
Conclusion: The number of supporting teeth may influence the accuracy of sCAIS in immediate implant surgery in the aesthetic dental region. Both groups showed acceptable clinical accuracy, but the surgical guide supported by six teeth may enhance implant accuracy compared to four.
Molecular beam epitaxial step-edge growth of integrated hetero-structures: Bi2Te3/multi-stepped Sb2Te3 nanoplate
Authors: Yi Gyu-Chul , Kim Yoonkang , Lee Sangmin , Lee Eunsu , Kim Seongbeom , Suh Wonwoo , Kim Imhwan , Jeon Junyeop , Kim Miyoung 
Published: September 2024

Abstract

We report the synthesis of multiple Bi2Te3 shells on multi-stepped Sb2Te3 nanoplates using molecular beam epitaxial (MBE) step-edge growth. For the growth of Bi2Te3/Sb2Te3 hetero-structures, multi-stepped Sb2Te3 nanoplates with stair-like morphology following layer-by-layer (LBL) growth mode were obtained by optimizing the growth temperature, and the growth of Bi2Te3 on the step-edges of the Sb2Te3 nanoplates was followed. Width of Bi2Te3 on the Sb2Te3 nanoplates was controlled by changing the growth time. Structural properties of the hetero-structures were investigated using aberration-corrected (Cs-corrected) high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), revealing the interface between Sb2Te3 and Bi2Te3. In-plane epitaxial relation at the interface was confirmed using fast Fourier transforms (FFTs). Compositional analysis of Bi2Te3 and Sb2Te3 was verified through energy-dispersive X-ray spectroscopy. Furthermore, we performed density functional theory (DFT) calculations to confirm the preferential growth of Bi2Te3 on the step-edges of Sb2Te3. By forming multi-stepped core structure, it would be feasible to create various integrated hetero-structures.
Dynamic Analysis of Nonlinear Models for Long-distance Belt Conveyors with Multi Motors
Authors: Zhang Yuan , Li Qiying , Jia Xianghui , Zhou Dan , Zhang Daoxian , Zhang Dongyue , Li Chenming 
Published: September 2024

Abstract

Based on the theory of viscoelasticity, a nonlinear dynamic model of the long-distance belt conveyor system is established considering PMSMs (Permanent Magnet Synchronous Motors). Then, a constraint following robust control is designed to accurately control the start and stop of the belt conveyor. The analysis then examines the speed, tension, and other parameters at critical positions during the startup phases with different desired speed curves. The parking phase is also investigated by dividing into four cases including unloaded free parking, fully loaded free parking, unloaded emergency parking, and fully loaded emergency parking. On this basis, four temperature conditions are set to study the slip ratio for the parking process: room temperature, extremely low temperature, monthly low temperature, and monthly high temperature. By comparing with traditional controllers, the proposed model-based underactuated controller can effectively improve the aforementioned metrics under various operating conditions, contributing to the safe and stable operation of long-distance nonlinear belt conveyors.
Impact of Motivation and Social Support on Elderly Engagement in Korean Archery: A TPB Approach
Authors: Yu Jae-gu , ‍Jeong Yunduk 
Published: September 2024

Abstract

Through the lens of the Theory of Planned Behavior (TPB), this study addresses key research gaps by investigating three primary objectives: (1) exploring the influence of participation motivation on attitudes; (2) examining the effects of attitudes, subjective norms, and perceived behavioral control (PBC) on behavioral intention; and (3) analyzing the moderating role of social support in these associations. Survey data were collected from amateur Han-gung athletes participating in ten senior Han-gung tournaments in South Korea. To ensure measurement scale robustness, confirmatory factor analysis, assessing factor loadings, average variance extracted (AVE), and construct reliability (CR) were conducted, with Cronbach’s alpha confirming scale reliability. The findings reveal the significant impact of participation motivation on attitudes and the positive effects of attitudes and PBC on behavioral intention. Moreover, social support emerges as a moderator in the relationship between subjective norms and behavioral intention. Academically, this study enriches the TPB framework by incorporating social support as a moderator, while practically, it offers insights for sports organizers and policymakers seeking to promote participation and engagement in Han-gung, particularly among the elderly population.