bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tracing the history and ecological context of Wolbachia double infection in a specialist host (Urophora cardui)—parasitoid (Eurytoma serratulae) system
Authors: Johannesen Jes 
Published: January 2017

AbstractThe endosymbiotic bacterium Wolbachia is the most widespread bacteria in insects, yet the ecology of novel acquisitions in natural host populations is poorly understood. Using temporal data separated by 12 years, I tested the hypothesis that immigration of a parasitoid wasp led to transmission of its Wolbachia strain to its dipteran host, resulting in double‐strain infection, and I used geographic and community surveys to explore the history of transmission in fly and parasitoid. Double infection in the fly host was present before immigration of the parasitoid. Equal prevalence of double infection in males and females, constant prevalence before and after immigration in two regions, and increase in one region of immigration indicate little if no competition between strains. Double infection was present throughout the fly's distribution range, but proportions varied highly (0–0.71, mean = 0.26). Two fly‐specific MLST strains, observed in Eastern and Western Europe, respectively, differed at hcpA only. Flies with either fly‐strain could be double infected with the parasitoid's strain. The geographic distribution of double infection implies that it is older than the fly host's extent distribution range and that different proportions of double infection are caused by demographic fluctuations in the fly. The geographic data in combination with community surveys of infections and strains further suggest that the parasitoid strain was the fly's ancestral strain that was transmitted to the parasitoid, that is, the reverse transmission route as first hypothesized. Based on these findings together with a comparison of oviposition strategies of other hosts harboring related Wolbachia strains, I hypothesize that trans‐infection during an insect host's puparial metamorphosis might be important in promoting horizontal transmission among diverse holometabolic taxa.
Edge responses are different in edges under natural versus anthropogenic influence: a meta‐analysis using ground beetles
Authors: Magura Tibor , Lövei Gábor L. , Tóthmérész Béla 
Published: January 2017

AbstractMost edges are anthropogenic in origin, but are distinguishable by their maintaining processes (natural vs. continued anthropogenic interventions: forestry, agriculture, urbanization). We hypothesized that the dissimilar edge histories will be reflected in the diversity and assemblage composition of inhabitants. Testing this “history‐based edge effect” hypothesis, we evaluated published information on a common insect group, ground beetles (Coleoptera: Carabidae) in forest edges. A meta‐analysis showed that the diversity‐enhancing properties of edges significantly differed according to their history. Forest edges maintained by natural processes had significantly higher species richness than their interiors, while edges with continued anthropogenic influence did not. The filter function of edges was also essentially different depending on their history. For forest specialist species, edges maintained by natural processes were penetrable, allowing these species to move right through the edges, while edges still under anthropogenic interventions were impenetrable, preventing the dispersal of forest specialists out of the forest. For species inhabiting the surrounding matrix (open‐habitat and generalist species), edges created by forestry activities were penetrable, and such species also invaded the forest interior. However, natural forest edges constituted a barrier and prevented the invasion of matrix species into the forest interior. Preserving and protecting all edges maintained by natural processes, and preventing anthropogenic changes to their structure, composition, and characteristics are key factors to sustain biodiversity in forests. Moreover, the increasing presence of anthropogenic edges in a landscape is to be avoided, as they contribute to the loss of biodiversity. Simultaneously, edges under continued anthropogenic disturbance should be restored by increasing habitat heterogeneity.
The impacts of precipitation increase and nitrogen addition on soil respiration in a semiarid temperate steppe
Authors: Zhang Xiaolin , Tan Yulian , Zhang Bingwei , Li Ang , Daryanto Stefani , Wang Lixin , Huang Jianhui 
Published: January 2017

AbstractSoil respiration, Rs, is strongly controlled by water availability in semiarid grasslands. However, how Rs is affected by precipitation change (either as rainfall or as snowfall) especially under increasing nitrogen (N) deposition has been uncertain. A manipulative experiment to investigate the responses of growing season Rs to changes in spring snowfall or summer rainfall with or without N addition was conducted in the semiarid temperate steppe of China during three hydrologically contrasting years. Our results showed that both spring snow addition and summer water addition significantly increased Rs by increasing soil moisture. The effect of spring snow addition only occurred in years with both relatively lower natural snowfall and later snowmelt time. Summer water addition showed a much stronger effect on Rs by increasing plant root growth and microbial activities, but the magnitude also largely depended on the possible legacy effect of previous year precipitation. Our results indicated that precipitation increase in the form of snowfall had weaker effects than that in the form of rainfall as the former only accounted for less than 30% of total precipitation. Compared with other ecosystem processes, Rs was less responsible for increase in N deposition as it did not increase root productivity and microbial activities in the soils. Our results provided field data constraints for modeling the ecosystem carbon balance under the future global change scenarios in semiarid grasslands.
Identifying the spatial pattern and importance of hydro‐geomorphic drainage impairments on unpaved roads in the northeastern USA
Authors: Wemple Beverley C. , Clark Gordon E. , Ross Donald S. , Rizzo Donna M. 
Published: January 2017

AbstractRoads have been widely studied as sources of runoff and sediment and identified as pollutant production sources to receiving waters. Despite the wealth of research on logging roads in forested, upland settings, little work has been conducted to examine the role of extensive networks of rural, low‐volume, unpaved roads on water quality degradation at the catchment scale. We studied a network of municipal unpaved roads in the northeastern US to identify the type and spatial extent of ‘hydro‐geomorphic impairments’ to water quality. We mapped erosional and depositional features on roads to develop an estimate of pollutant production. We also mapped the type and location of design interventions or best management practices (BMPs) used to improve road drainage and mitigate water quality impairment. We used statistical analyses to identify key controls on the frequency and magnitude of erosional features on the road network, and GIS to scale up from the survey results to the catchment scale to identify the likely importance of unpaved roads as a pollutant source in this setting. An average of 21 hydro‐geomorphic impairments were mapped per kilometer of road, averaging 0.3 m3in volume. Road gradient and slope position were key controls on the occurrence of these features. The presence of BMPs effectively reduced erosion frequency. Scaled up to the watershed and using a conservative estimate of road–stream connectivity, our results for the Winooski River watershed in the northeastern US suggest that roughly 16% and 6% of the average annual sediment and phosphorus flux, respectively, of the Winooski River may be derived from unpaved roads. Our study identifies an under‐appreciated source of water quality degradation in rural watersheds, provides insights into identifying ‘hot spots’ of pollutant production associated with these networks, and points to effectiveness of design interventions in mitigating these adverse impacts on water quality. Copyright © 2017 John Wiley & Sons, Ltd.
Impact of melatonin receptor‐signaling on Zeitgeber time‐dependent changes in cell proliferation and apoptosis in the adult murine hippocampus
Authors: Fredrich Michaela , Hampel Mareike , Seidel Kay , Christ Elmar , Korf Horst‐Werner 
Published: January 2017

ABSTRACTThe hippocampus is subjected to diurnal/circadian rhythms on both the morphological and molecular levels. Certain aspects of cell proliferation in the adult hippocampus are regulated by melatonin and accompanied by apoptosis to ensure proper tissue maintenance and function. The present study investigated Zeitgeber time (ZT)‐dependent changes in cell proliferation and apoptosis in the adult murine hippocampus and their regulation by melatonin receptor type1 and type2 (MT1/2)‐mediated signaling. Adult melatonin‐proficient C3H/HeN mice and melatonin‐proficient (C3H/HeN) mice with targeted deletion of MT1/2 were adapted to a 12‐h light, 12‐h dark photoperiod and were sacrificed at ZT00, ZT06, ZT12, and ZT18. Immunohistochemistry for Ki67 and activated caspase‐3 in combination with different markers for the diverse cell types residing in the hippocampus served to identify and quantify proliferating and apoptotic cells in the hippocampal subregions. ZT‐dependent changes in cell proliferation and apoptosis were found exclusively in the subgranular zone (SGZ) and granule cell layer (GCL) of melatonin‐proficient mice with functional MT1/2. Cell proliferation in the SGZ showed ZT‐dependent changes indicated by an increase of proliferating immature neurons during the dark phase of the 24‐h light‐dark cycle. Apoptosis showed ZT‐dependent changes in the SGZ and GCL indicated by an increase of apoptotic immature neurons at ZT06 (SGZ) and a decrease of immature and mature neurons at ZT18 (GCL). Our results indicate that ZT‐dependent changes in proliferation of immature neurons in the SGZ are counterbalanced by ZT‐dependent changes in apoptosis of immature and mature neurons in the SGZ and GCL exclusively in mice with functional MT1/2. Therefore, MT1/2‐mediated signaling appears to be crucial for generation and timing of ZT‐dependent changes in cell proliferation and apoptosis and for differentiation of proliferating cells into neurons in the SGZ. © 2017 Wiley Periodicals, Inc.
Determining annual cryosphere storage contributions to streamflow using historical hydrometric records
Authors: Brahney Janice , Menounos Brian , Wei Xiaohua , Curtis Paul Jefferson 
Published: January 2017

AbstractAlpine glaciers and perennial snow fields are important hydrologic elements in many mountain environments providing runoff during the late summer and during periods of drought. Because relatively long records of glacier mass–balance data are absent from many glacierized catchments, it remains unclear to what extent shrinking perennial snow and glaciers have affected runoff trends from these watersheds. Here, we employ a hydrograph separation technique that uses a double mass curve in an attempt to isolate changes in runoff due to glacier retreat and disappearance of perennial snow. The method is tested using hydrometric data from 20 glacierized and 16 nonglacierized catchments in the Columbia Basin of Canada. The resulting estimates on cryosphere storage contribution to streamflow were well correlated to other regional estimates on the basis of measurements as well as empirical and mechanistic models. Annual cryosphere runoff changed from +19 to −55% during the period 1975–2012, with an average decline of 26%. For August runoff, these changes ranged from +17 to −66%, with an average decrease of 24%. Reduction of cryosphere contributions to annual and late summer flows is expected to continue in the coming decades as glaciers and the perennial snow patches shrink. Our method to isolate changes in late summer cryospheric storage contributions can be used as a first order estimate on changes in glacier contributions to flow and may help researchers and water managers target watersheds for further analysis.
Cerebrospinal Fluid Proteins as Regulators of Beta‐amyloid Aggregation and Toxicity
Authors: Pate Kayla M. , Murphy Regina M. 
Published: January 2017

AbstractAmyloid disorders, such as Alzheimer's, are almost invariably late‐onset diseases. One defining diagnostic feature of Alzheimer's disease is the deposition of beta‐amyloid as extracellular plaques, primarily in the hippocampus. This raises the question: are there natural protective agents that prevent beta‐amyloid from depositing, and is it loss of this protection that leads to onset of disease? Proteins in cerebrospinal fluid (CSF) have been suggested to act as just such natural protective agents. Here, we describe some of the early evidence that led to this suggestion, and we discuss, in greater detail, two CSF proteins that have garnered the bulk of the attention.
Development of a Bio‐Inspired Dual Catalytic System for Alkane Dehydrogenation
Authors: West Julian G. , Sorensen Erik J. 
Published: January 2017

AbstractThe alkene is a central functional group in organic synthesis. While myriad reliable methods exist to access this moiety from other functionalities, acceptorless dehydrogenation, or the direct synthesis of alkenes from alkanes with hydrogen gas as the sole byproduct, remains a challenging, albeit highly desirable, transformation. This essay provides an account of our recent efforts toward accessing this difficult reaction class, with particular attention paid to the diverse precedents that informed our explorations. This report highlights the benefits of maintaining a broad range of interests, and we hope that it illustrates the vast connectivity between chemical disciplines.
Telescoped Synthesis of γ‐Bromo‐β‐Lactones from Allylic Bromides Employing Carbon Dioxide
Authors: Vellalath Sreekumar , Romo Daniel 
Published: January 2017

AbstractA direct synthesis of the title compounds involving a stepwise Zn‐mediated carboxylation of allylic bromides with CO2 delivering β, γ‐unsaturated carboxylic acids and a subsequent bromolactonization is reported. The described method demonstrates the use of readily prepared allylzinc bromides by the method of Knochel for fixation of CO2 employing commodity chemicals and zinc dust. This process was then optimized into a two‐stage, telescoped process for the direct synthesis of γ‐bromo‐β‐lactones from allyl bromides. The described strategy delivers functionalized β‐lactones with dual reactivity as acylating and alkylating agents, which have utility as synthetic intermediates and are attracting growing interest as proteomic tools for activity‐based protein profiling.
Excess Retinoic Acid Induces Fusion of Centra by Degenerating Intervertebral Ligament Cells in Japanese flounder, Paralichthys olivaceus
Authors: Wu Xiaoming , Chen Qiran , Washio Youhei , Yokoi Hayato , Suzuki Tohru 
Published: January 2017

ABSTRACTIn marine aquaculture fish, excessive supplement of vitamin A (VA) to zooplanktons for larval culture and experimental exposure of larvae to retinoic acid (RA: active form of VA) have been known to cause vertebral deformity. However, the tissues in the developing vertebral column that are affected by RA and the progression of vertebral deformity remain undetermined. To examine these questions, we histologically traced the progress of vertebral deformity induced by RA in Japanese flounder (Paralichthys olivaceus). Larvae were exposed to RA for 3 days at mid‐metamorphosis (G‐stage), a critical stage for vertebral deformity. Intervertebral ligament, which is known to form intervertebral joints in cooperation with the notochord, was severely degenerated by RA, leading to fusion of centra. During further development to adult, growth of centra was severely suppressed in an anterior–posterior direction in RA‐treated fish and the notochord tissue was lost from fused centra, resulting in complete loss of intervertebral joints and fusion of centra. We conclude that RA initially damages the intervertebral ligaments, and these defects lead to fusion, narrowing of centra, and loss of intervertebral joints in the vertebral column. The cumulative effect of these modifications is a truncated body form.
Tree‐Chromatic Number Is Not Equal to Path‐Chromatic Number*
Authors: Huynh Tony , Kim Ringi 
Published: January 2017

AbstractFor a graph G and a tree‐decomposition of G, the chromatic number of is the maximum of , taken over all bags . The tree‐chromatic number of G is the minimum chromatic number of all tree‐decompositions of G. The path‐chromatic number of G is defined analogously. In this article, we introduce an operation that always increases the path‐chromatic number of a graph. As an easy corollary of our construction, we obtain an infinite family of graphs whose path‐chromatic number and tree‐chromatic number are different. This settles a question of Seymour (J Combin Theory Ser B 116 (2016), 229–237). Our results also imply that the path‐chromatic numbers of the Mycielski graphs are unbounded.
Characterisation of the enzymatic properties of MpAPr1, an aspartic protease secreted by the wine yeast Metschnikowia pulcherrima
Authors: Theron Louwrens Wiid , Bely Marina , Divol Benoit 
Published: January 2017

AbstractBACKGROUNDMpAPr1, encoding an acid protease from the wine yeast Metschnikowia pulcherrima IWBT Y1123, was previously isolated and shown to display potential activity against casein and grape proteins. However, its characterisation remained partial.RESULTSMpAPr1 was cloned into the pGAPZαA vector and transformed into Komagataella pastoris X33 for heterologous expression. After verification of activity, the enzyme properties were characterised. Protease activity within the concentrated supernatant was retained over a pH range of 3.0 to 5.0 and between 10 °C and 50 °C. Optimal conditions for protease activity were found at 40 °C and pH 4.5. Activity was mostly unaffected by the presence of metal ions with the exception of Cu2+ and Ni2+. Furthermore, proteolytic activity was retained in the presence of sugar and ethanol. pH and temperature conditions for MpAPr1 expression in K. pastoris were optimised. Purification was achieved by means of cation exchange chromatography and kinetic parameters (Km and Vmax) were determined. MpAPr1 activity against grape proteins was confirmed, but the extent of the degradation was dependent on the nature of these proteins and the environmental conditions.CONCLUSIONOverall, the results suggest that MpAPr1 could be applied in food biotechnology processes such as winemaking. © 2017 Society of Chemical Industry
Traditional Agrarian Landscape in the Mediterranean Mountains. A Regional and Local Factor Analysis in the Central Spanish Pyrenees
Authors: Lasanta Teodoro , Errea María Paz , Nadal‐Romero Estela 
Published: January 2017

AbstractDevelopment of rural areas needs information about sustainable use of the land. The main objective of this research is to study the spatial distribution pattern of different field types in the traditional agricultural landscape in the Central Spanish Pyrenees (flat fields, four different terrace fields, sloping field and shifting agriculture) related to different geoecological variables: slope, exposition, altitude, distance to the villages, rainfall (intensity and volume), lithology and demographic pressure. Agricultural land patterns were mapped in five valleys in the Central Pyrenees from aerial photographs (1957). These maps were digitised and implemented in a Geographic Information System on a scale 1:50,000. We determined the influence of different geoecological variables in the spatial distribution of land agricultural patterns at local and regional valley using statistical analysis. The results showed that the agricultural area occupied a large extension, despite physical environment limitations, extending even in marginal slopes, with little agricultural skills, creating a complex landscape with different field patterns. At the local scale (valley), slope and distance are the more influence variables to explain the spatial distribution of the different field types. At the regional scale, in the Western valleys, sloping fields and shifting agriculture predominate, while terraces dominate in the Eastern valleys. Distance to the village and rainfall intensity is the more determinant factors in the spatial distribution. Copyright © 2017 John Wiley & Sons, Ltd.
An approach to correct the effects of phytoplankton vertical nonuniform distribution on remote sensing reflectance of cyanobacterial bloom waters
Authors: Xue Kun , Zhang Yuchao , Ma Ronghua , Duan Hongtao 
Published: January 2017

AbstractCyanobacterial blooms occur frequently in eutrophic lakes and their potentially harmful effects affected the security of drinking water and food sources, biodiversity, and economic activities, and attracted the attention of general public worldwide. Cyanobacteria could move vertically in the water column by regulating their buoyancy, which leads to the assumption of homogeneous water invalid. Ecolight, based on radiative transfer theory, was applied to examine the effects of vertical nonuniform of chlorophyll a concentrations (Chl a(z)) on remote sensing reflectance spectrum (Rrs(λ)) of optically complex inland waters. Simulations for nonuniform water consisting of three Chl a(z) profile classes, including Gaussian, exponential, and power, were compared with simulations for a reference homogeneous water whose Chl a was identical to average value of the nonuniform case. The near‐surface aggregation of phytoplankton are shown to have significant influence on Rrs(λ) and Chl a inversion algorithms. Variations of ΔRrs(λ) (relative difference of Rrs(λ) between inhomogeneous and homogeneous waters with same average Chl a concentration) mainly depended on the Chl a(z) structure parameters and wavelength. A correction scheme was developed based on the relationships between ΔRrs(λ) and Chl a(z) structure parameters. With knowledge of Chl a(z) profile parameters, Rrs(λ) of inhomogeneous waters can be corrected to the Rrs(λ) of uniform waters with same average Chl a across the water column. Examples of field data from Lake Chaohu illustrated the effects of phytoplankton variation on the near infrared‐to‐red ratio of Rrs and the Rrs correction performance.
Missing the target: including perspectives of women with overweight and obesity to inform stigma‐reduction strategies
Authors: Puhl R. M. , Himmelstein M. S. , Gorin A. A. , Suh Y. J. 
Published: January 2017

SummaryObjectivePervasive weight stigma and discrimination have led to ongoing calls for efforts to reduce this bias. Despite increasing research on stigma‐reduction strategies, perspectives of individuals who have experienced weight stigma have rarely been included to inform this research. The present study conducted a systematic examination of women with high body weight to assess their perspectives about a broad range of strategies to reduce weight‐based stigma.MethodsWomen with overweight or obesity (N = 461) completed an online survey in which they evaluated the importance, feasibility and potential impact of 35 stigma‐reduction strategies in diverse settings. Participants (91.5% who reported experiencing weight stigma) also completed self‐report measures assessing experienced and internalized weight stigma.ResultsMost participants assigned high importance to all stigma‐reduction strategies, with school‐based and healthcare approaches accruing the highest ratings. Adding weight stigma to existing anti‐harassment workplace training was rated as the most impactful and feasible strategy. The family environment was viewed as an important intervention target, regardless of participants' experienced or internalized stigma.ConclusionThese findings underscore the importance of including people with stigmatized identities in stigma‐reduction research; their insights provide a necessary and valuable contribution that can inform ways to reduce weight‐based inequities and prioritize such efforts.