bluuebunny/crossref_metadata_2025_split huggingface dataset:
Naringenin‐responsive riboswitch‐based fluorescent biosensor module for Escherichia coli co‐cultures
Authors: Xiu Yu , Jang Sungho , Jones J. Andrew , Zill Nicholas A. , Linhardt Robert J. , Yuan Qipeng , Jung Gyoo Yeol , Koffas Mattheos A. G. 
Published: May 2017

ABSTRACTThe ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high‐throughput rapid screening techniques is of upmost importance for the future of synthetic biology and metabolic engineering. Here we describe the development of an RNA riboswitch‐based biosensor module with dual fluorescent reporters, and demonstrate a high‐throughput flow cytometry‐based screening method for identification of naringenin over producing Escherichia coli strains in co‐culture. Our efforts helped identify a number of key operating parameters that affect biosensor performance, including the selection of promoter and linker elements within the sensor‐actuator domain, and the effect of host strain, fermentation time, and growth medium on sensor dynamic range. The resulting biosensor demonstrates a high correlation between specific fluorescence of the biosensor strain and naringenin titer produced by the second member of the synthetic co‐culture system. This technique represents a novel application for synthetic microbial co‐cultures and can be expanded from naringenin to any metabolite if a suitable riboswitch is identified. The co‐culture technique presented here can be applied to a variety of target metabolites in combination with the SELEX approach for aptamer design. Due to the compartmentalization of the two genetic constructs responsible for production and detection into separate cells and application as independent modules of a synthetic microbial co‐culture we have subsequently reduced the need for re‐optimization of the producer module when the biosensor is replaced or removed. Biotechnol. Bioeng. 2017;114: 2235–2244. © 2017 Wiley Periodicals, Inc.
Leaping‐in and leaping‐ahead: A hermeneutic phenomenological study of being‐responsible in psychotherapeutic supervision
Authors: Glover Rita , Philbin Mark 
Published: May 2017

AbstractAimsPsychotherapeutic supervision is a human‐to‐human encounter in which counsellors and psychotherapists regularly present their client case work. There are multiple theoretical and practical perspectives about what supervision is and how to do it. Yet, little is known about the day‐to‐day lived experiences of psychotherapeutic supervisors. This article reports on a key finding from a Heideggerian hermeneutic phenomenological research study which was concerned with uncovering what it is really like being a psychotherapeutic supervisor. Participants told many stories about experiences of heightened responsibility. They recounted how experiences of anxiety in specific supervisory encounters were intertwined with burdensome experiences of responsibility. Existing theories, practices and prescribed ethical guidelines on managing supervisory responsibility were of limited assistance to supervisors grappling with troubling supervisory experiences. Instead, to cope with the burden of responsibility, psychotherapeutic supervisors had common human experiences of ‘leaping‐in and leaping‐ahead’ of their supervisees, leading to hidden and unintended consequences for supervisees and supervisors.ConclusionsResponsibility is inescapable in supervisory encounters and is fuelled by a mood of anxiety. The phenomenon of responsibility could be rendered more intelligible, in supervision consultation, by privileging, uncovering and dwelling in psychotherapeutic supervisors’ day‐to‐day lived experience of being‐responsible, while being mindful of what we already know about responsible supervisory practice.
The effects of virtual human's spatial and behavioral coherence with physical objects on social presence in AR
Authors: Kim Kangsoo , Maloney Divine , Bruder Gerd , Bailenson Jeremy N. , Welch Gregory F. 
Published: May 2017

AbstractIn augmented reality, people can feel the illusion of virtual humans (VHs) integrated into a real (physical) space. However, affordances of the real world and virtual contents might conflict, for example, when the VHs and real objects “collide” by occupying the same space. This implausible conflict can cause a break in presence in real–virtual human interactions. In this paper, we address an effort to avoid this conflict by maintaining the VH's spatial and behavioral coherence with respect to the physical objects or events (e.g., natural occlusions and appropriate help‐requesting behaviors to avoid implausible physical–virtual collisions). We present a human subject experiment examining the effects of the physical–virtual coherence on human perceptions, such as social/copresence and behaviors with the VH. The basic ideas, experimental design, and results supporting the benefit of the VH's spatial and behavioral coherence are presented and discussed.
Supervised coordinate descent method with a 3D bilinear model for face alignment and tracking
Authors: Zhang Yongqiang , Liu Shuang , Yang Xiaosong , Zhang Jianjun , Shi Daming 
Published: May 2017

AbstractFace alignment and tracking play important roles in facial performance capture. Existing data‐driven methods for monocular videos suffer from large variations of pose and expression. In this paper, we propose an efficient and robust method for this task by introducing a novel supervised coordinate descent method with 3D bilinear representation. Instead of learning the mapping between the whole parameters and image features directly with a cascaded regression framework in current methods, we learn individual sets of parameters mappings separately step by step by a coordinate descent mean. Because different parameters make different contributions to the displacement of facial landmarks, our method is more discriminative to current whole‐parameter cascaded regression methods. Benefiting from a 3D bilinear model learned from public databases, the proposed method can handle the head pose changes and extreme expressions out of plane better than other 2D‐based methods. We present the reliable result of face tracking under various head poses and facial expressions on challenging video sequences collected online. The experimental results show that our method outperforms state‐of‐art data‐driven methods.
3D skeleton‐based action recognition by representing motion capture sequences as 2D‐RGB images
Authors: Laraba Sohaib , Brahimi Mohammed , Tilmanne Joëlle , Dutoit Thierry 
Published: May 2017

AbstractIn recent years, 3D skeleton‐based action recognition has become a popular technique of action classification, thanks to development and availability of cheaper depth sensors. State‐of‐the‐art methods generally represent motion sequences as high dimensional trajectories followed by a time‐warping technique. These trajectories are used to train a classification model to predict the classes of new sequences. Despite the success of these techniques in some fields, particularly when the data used are captured by a high‐precision motion capture system, action classification is still less successful than the field of image classification, especially with the advance of deep learning. In this paper, we present a new representation of motion sequences (Seq2Im—for sequence to image), which projects motion sequences onto the RGB domain. The 3D coordinates of joints are mapped to red, green, and blue values, and therefore, action classification becomes an image classification problem and algorithms for this field can be applied. This representation was tested with basic image classification algorithms (namely, support vector machine, k‐nearest neighbor, and random forests) in addition to convolutional neural networks. Evaluation of the proposed method on standard 3D human action recognition datasets shows its potential for action recognition and outperforms most of the state‐of‐the‐art results.
Real‐life clinical outcomes with everolimus eluting platinum chromium stent with an abluminal biodegradable polymer in patients from the Swedish Coronary Angiography and Angioplasty Registry (SCAAR)
Authors: Sarno Giovanna , Lagerqvist Bo , Olivecrona Göran , Varenhorst Christoph , Danielewicz Mikael , Hambraeus Kristina , Lindholm Daniel , Råmunddal Truls , Witt Nils , James Stefan 
Published: May 2017

BackgroundNo previous studies have evaluated the performance of the Synergy stent in a large real‐life population.ObjectivesTo describe the initial real‐life experience with a novel everolimus eluting platinum chromium stent with abluminal biodegradable polymer (SYNERGY) in unselected patients from a nationwide registry.MethodsAll implanted Synergy stents were compared with other new generation drug eluting stents (n‐DES) with >1,000 implantations in Sweden between March 2013 and October 2015. Restenosis, definite stent thrombosis (ST), myocardial infarction (MI) and death rates were assessed using propensity score and Cox regression analyses.ResultsA total of 7,886 of Synergy stents and 64,429 other n‐DES (BioMatrix, N = 1,953; Orsiro, N = 4,946; Promus Element Plus, N= 2,543; Promus Premier, N= 20,414; Xience Xpedition, N= 7,971, Resolute/Resolute Integrity, N = 19,021; Ultimaster, N = 1,156; Resolute Onyx, N = 6,425) were implanted in 42,357 procedures.Restenosis and stent thrombosis occurred in 642 and 314 cases, respectively, in the overall population at 1 year. The cumulative rate of restenosis (1.1% vs. 1.0%, adjusted HR: 1.24 95% CI: 0.88–1.75; P = 0.21) and ST (0.4% vs. 0.5%, adjusted HR: 0.97; 95% CI: 0.63–1.50; P = 0.17) up to 1 year was low in both the Synergy group and the other n‐DES group. Death occurred in 5.2% versus 4.5% (adjusted HR: 1.14; 95% CI: 0.96–1.36; P = 0.11) and MI in 3.2% versus 3.5%, (adjusted HR: 1.11; 95% CI: 0.93–1.33; P = 0.24) up to 1 year.ConclusionsIn a large real‐life population the Synergy stent appears to be safe and effective with a low rate of restenosis and ST comparable with other n‐DES. © 2017 Wiley Periodicals, Inc.
Safety and efficacy outcomes of 3rd generation DES in an all‐comer population of patients undergoing PCI: 12‐month and 24‐month results of the e‐Biomatrix French registry
Authors: Maupas Eric , Lipiecki Janusz , Levy Raphy , Faurie Benjamin , Karsenty Bernard , Moulichon Marc Eric , Brunelle François , Maillard Luc , de Poli Fabien , Lefèvre Thierry 
Published: May 2017

ObjectivesThe French Ebiomatrix registry aimed to confirm the results of the Leaders trial in an all‐comer population in France.BackgroundThe Leaders trial showed the Biolimus‐eluting‐sent (BES) to be equivalent to the Cypher stent in terms of safety and efficacy at 1 year and superior regarding stent thrombosis after 1 year.MethodsBES recipients were enrolled in 42 French centers with up to 24‐month clinical follow up.Results2365 patients were included. Mean age: 65.7 ±11.2 years, 76.1% males, 31.8% had diabetes, 36.5% ACS (28.7% non‐ST‐elevation MI and 7.8% with ST‐elevation MI). 1.7 ± 1.0 stents/patient were implanted and procedural success was 99.5%. 12‐month follow‐up was completed in 94.3% patients and 24 months in 91.4%. MACCE rates at 12 and 24 months were 5.8% and 9% (all cause‐death 1.5% and 2.2%; stent thrombosis definite/probable 0.4% and 0.6%), respectively. MACCE were not significantly higher in diabetic patients compared with non‐diabetics but cardiac death was higher (1.6% vs. 0.6%, P = 0.01 at 1 year and 1.9% vs. 0.6, P = 0.005 at 2 years) as was stent thrombosis (0.9% vs. 0.2%, P = 0.009 and 1.2 vs. 0.3% P = 0.008). Compared with non‐ACS patients, MACCE was significantly higher in the ACS subgroup (7.5% vs. 4.8%, P = 0.001 at 1 year and 10.3% vs.8.1%, P = 0.07 at 2 years).ConclusionIn this large real‐world registry, the BES with biodegradable polymer showed excellent acute and mid‐term outcomes with a 5.8% and 9% rate of MACCE at one and 2 years and a very low rate of stent thrombosis between 1 and 2 years (0.2%), thus demonstrating the replicability of the LEADERS trial in a registry population. © 2017 The Authors Catheterization and Cardiovascular Interventions Published by Wiley Periodicals, Inc.
Prognostic significance of aortic valve gradient in patients with severe aortic stenosis undergoing transcatheter aortic valve replacement
Authors: Witberg Guy , Finkelstein Arik , Barbash Issi , Assali Abid , Shapira Yaron , Segev Amit , Halkin Amir , Fefer Paul , Ben‐Shoshan Jeremy , Konigstein Maayan , Sagie Alexander , Guetta Victor , Kornowski Ran , Barsheshet Alon 
Published: May 2017

AbstractObjectiveTo evaluate the effect of baseline aortic valve gradient (AVG) both as a continuous and a categorical variable on mortality in patients undergoing transcatheter aortic valve replacement (TAVR), focusing on the high‐gradient severe aortic stenosis (AS) patients.BackgroundIdentifying new predictors of mortality in the TAVR population can help refine risk stratification and improve the patient selection process for this procedure. So far, AVG has mainly been studied as a categorical variable and there is a paucity of data on its prognostic value as a continuous variable, especially in patients with high AVG AS, who constitute the majority of patients referred for TAVR.MethodsWe analyzed data on 1,224 consecutive symptomatic severe AS patients, who underwent TAVR at 3 centers. The relation between pre‐TAVR AVG and mortality was evaluated among all patients and in patients with high AVGs (mean AVG ≥40 mm Hg) using the Cox proportional hazard model adjusting for multiple variables.ResultsDuring a mean follow‐up of 1.8 years, baseline AVG was inversely associated with mortality in the entire cohort and in patients with high AVG AS. By multivariable analysis, patients with mean AVG 40–60 mm Hg and >60 mm Hg had a respective 38% (P = 0.010) and 61% (P < 0.001) reduction in mortality compared to patients with mean AVG <40 mm Hg. Every 10 mm Hg increase in mean AVG was associated with 20% reduction in mortality (P < 0.001). Analyses among patients with high (mean AVG >40 mm Hg) and very high AVG AS (mean AVG >60 mm Hg) yielded similar results (HR = 0.88, P = 0.031, and HR = 0.80, P = 0.019, per 10 mm Hg increase in AVG, respectively). Using peak AVGs and an analysis restricted to patients without reduced ejection fraction yielded consistent results.ConclusionsBaseline AVGs show an inverse association with mortality post‐TAVR. These results were consistent also in patients with high‐gradient AS, suggesting that AVG can be used to identify patients most likely to benefit from TAVR.
Mesoporous and Skeletal Molybdenum Carbide for Hydrogen Evolution Reaction: Diatomite‐Type Structure and Formation Mechanism
Authors: Wang Yangxia , Shi Zhangping , Mo Qijie , Gao Boxu , Liu Bolun , Wang Lei , Zhang Yahong , Gao Qingsheng , Tang Yi 
Published: May 2017

AbstractMolybdenum carbide (MoCx) has generated mounting attentions as noble‐metal‐free electrocatalysts for hydrogen evolution reaction (HER). Herein, triggered by the strategy of organic‐inorganic hybrid oriented transformation in nature, we attempt to prepare a diatomite‐type mesostructured MoCx electrocatalyst via the solid‐state reaction between MoOx/DMF hybrid precursor and β‐cyclodextrin. Benefiting from the occurrence of the designed individual “mesoskeleton‐forming” and “MoCx‐generating” processes during pyrolysis, we successfully harvest the targeted electrocatalyst with mesostructural skeleton composed of inter‐connected MoCx nanoparticles (∼20 nm) and nanorod‐like morphology inheriting from its hybrid precursor. This crosslinked mesostructure can not only greatly decrease the diffusion resistance of reactants/products and promote the exposure of active sites, but also efficiently decrease the contact electrical resistance between neighboring MoCx nanoparticles. Importantly, the heterostructure of Mo2C and MoC on the nanorod is tunable in the synthesis process, which is proved to be favorable for the promoted HER performance in both acid and alkaline media. The strategy for synthesizing such nanostructures may open up new avenues for developing mesoskeletal materials
Synthesis of Benzodihydrofurans by Asymmetric C−H Insertion Reactions of Donor/Donor Rhodium Carbenes
Authors: Lamb Kellan N. , Squitieri Richard A. , Chintala Srinivasa R. , Kwong Ada J. , Balmond Edward I. , Soldi Cristian , Dmitrenko Olga , Castiñeira Reis Marta , Chung Ryan , Addison J. Bennett , Fettinger James C. , Hein Jason E. , Tantillo Dean J. , Fox Joseph M. , Shaw Jared T. 
Published: May 2017

AbstractMetal carbenes appended with two electron‐donating groups, known as “donor/donor” carbenes, undergo diastereo‐ and enantioselective rhodium‐catalyzed C−H insertion reactions with ether substrates to form benzodihydrofurans. Unlike the reactions of metal carbenes with electron‐withdrawing groups attached, the attenuated electrophilicity enables these reactions to be conducted in Lewis basic solvents (e.g., acetonitrile) and in the presence of water. The diazo precursors for these species are prepared in situ from hydrazone using a mild and chemoselective oxidant (MnO2). Although this sequence often can be performed in one‐pot, control experiments have elucidated why a “two‐pot” process is often more efficient. A thorough screening of achiral catalysts demonstrated that sterically encumbered catalysts are optimal for diastereoselective reactions. Although efficient insertion into allylic and propargylic C−H bonds is observed, competing dipolar cycloaddition processes are noted for some substrates. The full substrate scope of this useful method of benzodihydrofuran synthesis, mechanisms of side reactions, and computational support for the origins of stereoselectivity are described.
Stereospecific Winding of Polycyclic Aromatic Hydrocarbons into Trinacria Propellers
Authors: Mosca Dario , Stopin Antoine , Wouters Johan , Demitri Nicola , Bonifazi Davide 
Published: May 2017

AbstractThe stereospecific trimerization of enantiomerically pure binaphthols with hexakis(bromomethyl)benzene gives access in one step to enantiomerically pure molecular propellers, in which three binaphthyl rings are held together with dioxecine rings. X‐ray diffraction analysis revealed that three out the six naphthyl moieties are folded in a (EF)3‐type arrangement held by three intramolecular C−H⋅⋅⋅π interactions. This slips outward the three remaining naphthyl rings in a blade‐like fashion, just like in three‐folded propeller components. This peculiar conformation shows striking similarity to the mythological Sicilian symbol of Trinacria, from which the name “trinacria propeller” derives. The propeller conformation is also preserved in chlorinated solutions, as displayed by the presence of a peak at 4.7 ppm typical of an aromatic proton resonance engaged in a C−H⋅⋅⋅π interaction. The denaturation of the propeller‐like conformation is obtained at high temperature, corresponding to activation energy for the ring inversion of ca. 18.2 kcal mol−1. Notably, halide‐functionalized molecular propellers exposing I‐atoms at the leading and trailing edges could be prepared stereo‐ and regiospecifically by choosing the relevant iodo‐bearing BINOL derivative.
Cover Picture: Improving the Catalytic Activity of Au25 Nanocluster by Peeling and Doping (Chin. J. Chem. 5/2017)
Authors: Li Man‐bo , Tian Shi‐kai , Wu Zhikun 
Published: May 2017

The cover picture shows the structure transformation from a well‐known gold nanocluster Au25(SCH2CH2Ph)18 (Au25 for short) to a novel cadmium doped gold nanocluster [Au13Cd2(PPh3)6(SC2H4Ph)6(NO3)2]2Cd(NO3)4 (Au26Cd5 for short) by a combined peeling and doping process. Although the mother nanocluster Au25 is inactive, the as‐prepared Au26Cd5 exhibits high catalytic activity, as well as good recyclability and substrate tolerance at room temperature. The high catalytic activity is attributed to the cooperation between the exerted cadmium atoms and the neighbor gold atoms in the Au13 icosahedron based on some experimental results. More details are discussed in the article by Wu et al. on page 567–571.
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Predictive value of inflammatory factors on contrast‐induced acute kidney injury in patients who underwent an emergency percutaneous coronary intervention
Authors: Yuan Ying , Qiu Hong , Hu Xiaoying , Luo Tong , Gao Xiaojin , Zhao Xueyan , Zhang Jun , Wu Yuan , Qiao Shubin , Yang Yuejin , Gao Runlin 
Published: May 2017

BackgroundContrast‐induced acute kidney injury (CI‐AKI) is one of the most serious complications in patients who undergo percutaneous coronary intervention (PCI), especially in those with acute coronary syndrome. It has been shown that inflammation may play an important role in the pathophysiology of CI‐AKI.HypothesisInflammatory factors may play a predominant role in the prediction of CI‐AKI in patients who undergo emergency PCI.MethodsPatients who underwent emergency PCI from 2013 to 2015 were consecutively enrolled and were divided into CI‐AKI and non–CI‐AKI groups. Logistic analysis was used to identify the risk factors of CI‐AKI. Receiver operator characteristic curve analysis was performed to evaluate the area under the curve (AUC) and to establish the optimal cutoff.ResultsA total of 1061 patients were included, and the CI‐AKI rate was 5.47% (58/1061). Logistic analysis showed that the white blood cell (WBC) count (odds ratio [OR]: 1.103, 95% confidence interval [CI]: 1.018‐1.195, P = 0.016), neutrophil (N) count (OR: 1.134, 95% CI: 1.045‐1.232, P = 0.003), neutrophil to lymphocyte ratio (NLR) (OR: 1.105, 95% CI: 1.044‐1.169, P = 0.001), C‐reactive protein (CRP) level (OR: 1.006, 95% CI: 1.001‐1.011, P = 0.020), high‐sensitivity C‐reactive protein (hs‐CRP) level (OR: 1.099, 95% CI: 1.020‐1.184, P = 0.013), and big endothelin‐1 (ET‐1) level (OR: 4.030, 95% CI: 1.989‐8.165, P < 0.001) were all significant predictors for CI‐AKI, as was the left ventricular ejection fraction and diuretic administration. The AUC of the big ET‐1 level was the highest (0.793, 95% CI: 0.733‐0.853), followed by the NLR (0.708, 95% CI: 0.641‐0.774), hs‐CRP level (0.705, 95% CI: 0.627‐0.782), CRP level (0.684, 95% CI: 0.607‐0.761), N count (0.655, 95% CI: 0.584‐0.726), WBC count (0.620, 95% CI: 0.544‐0.695), and erythrocyte sedimentation rate (0.611, 95% CI: 0.527‐0.695).ConclusionsThe WBC count, N count, NLR, CRP level, hs‐CRP level, and big ET‐1 level are all associated with an increased risk of CI‐AKI, and among which, the big ET‐1 level, NLR, and the hs‐CRP level might have high predictive value for CI‐AKI after an emergency PCI.