bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efektivitas penghambatan enzim xanthin oksidase fraksi etanol, etil asetat dan n-heksane dari ekstrak etanol daun bakau kurap (Rhizophora Stylosa Griff.)
Authors: Raharjo Danang , Lisyani Tiara Ajeng , Wulandari Nia Isa 
Published: September 2024

Xanthin oksidase memiliki peranan penting dalam proses pembentukan asam urat dengan mengkatalisis berturut-turut hypoxanthine menjadi xanthine kemudian asam urat. Salah satu tumbuhan yang bisa dimanfaatkan sebagai antihiperurisemia adalah daun bakau kurap karena memiliki senyawa flavonoid, flavonol, flavon, isoplavon, flavonon, polifenol, alkaloid, tanin, glikosia dan tripenoid. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas penghambatan enzim xanthin oksidase dari ekstrak, fraksi dan isolat daun bakau kurap. Ekstrak dibuat dengan metode maserasi menggunakan pelarut etanol 96%. Pada isolasi enzim dilakukan menggunakan susu sapi segar dengan hasil aktivitas enzim didapatkan sebesar 0,0013 U/mL dari fraksi residu dan 0,002 U/mL dari hasil fraksi supernatan. Hasil dari penelitian ini senyawa yang terkandung didalam ektrak etanol daun bakau kurap yaitu senyawa metabolit sekunder senyawa alkaloid, flavonoid, saponin, terpenoid, dan tanin, dan senyawa aktif yang bertanggung jawab terhadap aktivitas penghambatan enzim xanthin oksidase adalah kuersetin hasil dari isolat C, pada uji aktivitas penghambatan ekstrak etanol, fraksi dan isolat daun bakau kurap yang dilihat dari IC50 sebesar 30,905 pada ekstrak etanol, fraksi polar etanol sebesar 39,722, fraksi semi polar etil asetat sebesar 16,361, fraksi non polar n-Heksan sebesar 61,734 dan isolat c sebesar 15,023. Berdasarkan dari penelitian ini dapat disimpulkan bahwa senyawa aktif yang terkandung di dalam daun bakau kurap yaitu kuersetin yang dibuktikan dengan nilai IC50 sebesar 15,023.

Kata kunci: Hiperurisemia, Isolasi Enzim ,Rhizophora Stylosa, Xanthin Oksidase
Pengukuran aktivitas antioksidan ekstrak etanol buah matoa (Pometia pinnata) dengan metode DPPH (2,2-difenil-1,1-pikrilhidrazil)
Authors: Sulistiani Sulistiani , Leswana Nurillahi Febria , Linden Susana 
Published: September 2024

Tanaman matoa dimanfaatkan oleh Bangsa Asia (Indonesia dan Malaysia) sebagai salah satu obat tradisional yang diketahui mengandung senyawa kimia berupa flavonoid, tanin, dan saponin. Berdasarkan latar belakang tersebut, rumusan masalah dalam penelitian ini yaitu bagaimana aktivitas antioksidan dari ekstrak etanol buah matoa (Pometia pinnata). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antioksidan ekstrak etanol buah matoa (Pometia pinnata) dengan metode DPPH. Penelitian ini dilakukan pada bulan Juni-Juli 2023. Aktivitas antioksidan ekstrak buah matoa dianalisis menggunakan alat spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil penelitian yang diperoleh menunjukkan nilai IC50 ekstrak buah matoa sebesar 181,551 ppm sedangkan larutan pembanding vitamin C sebesar 5,807 ppm. Nilai IC50 tersebut dapat ditentukan bahwa ekstrak etanol buah matoa merupakan antioksidan yang bersifat lemah.

Kata Kunci: Buah matoa, DPPH, antioksidan, Pometia pinnata
Pengaruh konsentrasi pelarut etanol terhadap kadar flavonoid total ekstrak krokot magenta (Portulaca grandiflora) dengan spektrofotometer UV-Vis
Authors: Kinasih Yosefin Dian Eka , Indriasari Christina 
Published: September 2024

Tanaman Krokot merupakan salah satu tanaman di Indonesia yang berkhasiat sebagai tanaman obat. Jenis krokot yang sering ditemui di masyarakat salah satunya adalah Portulaca grandiflora atau krokot varietas bunga magenta. Efek farmakologis dari krokot yaitu sebagai antibakteri, antiiflamasi, antioksidan, immunomodulator dan analgetik. Tujuan dilakukannya penelitian ini adalah untuk mengetahui perbedaan kadar flavonoid total ekstrak krokot magenta yang diekstraksi menggunakan konsentrasi pelarut etanol yang berbeda (50%, 70% dan 96%). Penetapan kadar flavonoid dilakukan menggunakan spektrofotometri UV-Vis. Metode ekstraksi yang digunakan adalah maserasi. Pengukuran absorbansi dilakukan menggunakan spektrofotometri UV-Vis pada panjang gelombang maksimal 424,6 nm. Berdasarkan hasil pengujian kadar flavonoid ekstrak krokot magenta didapatkan kadar flavonoid ekstrak krokot dengan pelarut etanol 96% yaitu sebesar 2,0872% b/b, ekstrak etanol 50% sebesar 1,1189% b/b dan ekstrak etanol 70% sebesar 1,3553% b/b. Hasil uji statistik menggunakan uji Anova menunjukkan adanya perbedaan yang signifikan.

Kata Kunci: Kadar flavonoid, konsentrasi etanol, krokot magenta
Analisis penggunaan obat resep dokter pasien Generalized Anxiety Disorder (GAD) di RSUD Andi Djemma Masamba
Authors: Yuli Nurvianthi Riska , Djafar Tanwir , Jehanam Tonsisius , Delta Delta , Safitri Alma 
Published: September 2024

Gangguan Cemas Menyeluruh adalah salah satu jenis gangguan mental dengan prevalensi sebanyak 12,8% di Sulawesi Selatan. Pemberian obat anticemas harus secara cermat (pemilihan jenis obat dan pengaturan regimen dosis). Penelitian ini bertujuan untuk menganalisa dan menggambarkan pola penggunaan obat anticemas pada kasus gangguan cemas menyeluruh dikaitkan dengan data klinik pasien di Poli Jiwa RSUD Andi Djemma Masamba Metode yang digunakan dalam penelitian ini adalah secara observasional retrospektif pada periode 4 April hingga 27 Juni 2023 di Poli Jiwa Rumah Sakit Umum Daerah Andi Djemma Masamba. Hasil penelitian menunjukkan dari 40 pasien mayoritas terdiri dari perempuan (70%) dengan rentang usia terbanyak yaitu 35 - 44 tahun (35%). Pasien paling banyak bertempat tinggal di Masamba (15%). Terapi yang diterima pasien GAD adalah obat anticemas golongan benzodiazepin meliputi alprazolam (43%), lorazepam (26%), klobazam (21%), dan diazepam (10%). Kesimpulannya, pemilihan obat dan pemberian dosis telah sesuai dengan pustaka dan dari segi kesesuaian dosis, tidak ditemukan problema obat yang berpotensi terjadi adalah efek samping seperti rasa mengantuk, depresi, sakit kepala, ataksia, insomnia, halusinasi dan mual. Sehingga diharapkan selanjutnya melibatkan sampel yang lebih besar serta menggunakan metode penelitian yang lebih spesifik.

Kata kunci: : anticemas, cemas, obat, problema obat
Gambaran perilaku vulva hygiene dan swamedikasi yang dilakukan oleh mahasiswi UNIDA Gontor dalam mengatasi keputihan
Authors: Marfu'ah Nurul , Khodijah Alisya , Fatihah Nadia Iha 
Published: September 2024

Keputihan adalah salah satu permasalah yang sering dialami wanita. Gangguan ini dapat disebabkan karena perilaku vulva hygiene yang kurang tepat seperti menggunakan celana dalam yang tidak menyerap keringat, jarang mengganti pembalut, dan cara membersihkan daerah kewanitaan yang kurang tepat. Berdasarkan latar belakang di atas, maka penelitian ini dilakukan dengan tujuan untuk mengetahui prevalensi, gambaran perilaku vulva hygiene, dan swamedikasi penyakit keputihan pada mahasiswi UNIDA Gontor. Penelitian ini merupakan penelitian non eksperimental yang dilakukan dengan metode observasional menggunakan kuisioner dan wawancara. Teknik pengambilan sampel dilakukan menggunakan metode purposif sampling sehingga diperoleh 95 orang mahasiswi sebagai responden (yang mengalami keputihan normal maupun tidak normal). Data yang didapatkan kemudian diberi skor menggukan 4 skala linkert dan selanjutnya dipersenkan. Analisis data menggunakan metode deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa prevalensi keputihan pada mahasiswi tahun 2021 dari jumlah mahasiswi sebanyak 1.652 orang, terdapat mahasiswi yang jarang mengalami keputihan sebanyak 82% (1.348 orang), sering mengalami keputihan sebanyak 15% (257 orang), dan tidak pernah mengalami keputihan sebanyak 3% (47 orang). Perilaku vulva hygiene pada mahasiswi dari 95 responden didapatkan hasil perilaku dalam menjaga kebersihan organ kewanitaan kategori sangat baik sebanyak 65,26% dan kategori baik sebanyak 34.74%. Swamedikasi yang dilakukan mahasiwi dari 95 responden didapatkan hasil mahasiswi yang dapat melakukan swamedikasi dalam mengatasi keputihan kategori sangat baik sebanyak 80% dan kategori baik sebanyak 20%.

Kata Kunci: keputihan, vulva higiene, swamedikasi
Modified Low-Field NMR Method for Improved Pore Space Analysis in Tight Fe-Bearing Siliciclastic and Extrusive Rocks
Authors: Fajt Michał , Fheed Adam , Machowski Grzegorz , Sowiżdżał Anna , Krzyżak Artur T. 
Published: September 2024

Abstract
Understanding the filtration and storage properties of tight reservoirs is crucial for efficient resource exploitation, particularly in unconventional formations. This study presents two low-field nuclear magnetic resonance (LF-NMR) techniques: standard cut-off and modified differential approaches combined with mercury injection capillary pressure (MICP) and X-ray diffraction (XRD) studies to evaluate porosity and pore size distribution (PSD) in such formations. The differential technique involves subtracting the dry sample signal from a 100% water-saturated one, allowing the chemically bound water compound to be eliminated and facilitating PSD analysis. Through the application of the percolation theory, we established a power–law relationship between LF-NMR transverse relaxation time (T2) and MICP pore-throat diameter, enabling the derivation of PSD and pseudo capillary pressure curves. Our methodology was validated on a sample set representing tight sandstones, conglomerates, and extrusive rocks with high clay and iron mineral content, demonstrating the superior accuracy of the modified differential method in estimating effective porosity and absolute PSD in comparison with the standard approach. While the use of the percolation theory in PSD conversion was successful for rocks with unimodal distributions, it often failed for rocks with larger voids. The study also revealed that the relationship between the LF-NMR transverse relaxation times and MICP pore sizes is both nonlinear and challenging to describe with a universal equation, especially in the presence of para- and ferro-magnetic elements in the rock matrix. Despite obstacles to the complete elimination of the influence of these minerals on the T2 distribution, employing the modified differential LF-NMR method significantly mitigated this effect and offered a precise and noninvasive way of characterizing the petrophysical properties of tight reservoir rocks. Consequently, our studies offer a significant step toward a more precise assessment of pore structures in unconventional reservoirs that could be translated into more efficient strategies for locating geothermal heat and hydrocarbon resources.
External Quality Assessment Scheme for Transfusion Transmissible Infections among Blood Service Facilities in the Philippines, 2015
Authors: Yu Rhoda , Chamen Iza Mae , Punzalan Kenneth Aristotle , De Vera III Benjamin 
Published: September 2024

The External Quality Assessment Scheme (EQAS) for Blood Screening Serology aims to raise standards and assess the phases of laboratory testing of blood units.
In 2015, the National Blood Program listed a total of 200 Blood Service Facilities (BSF)147 of which enrolled for EQAS. These participants were given an EQAS panel designed to check the capacity of a BSF to detect the 5 transfusion transmitted infections (HIV, HBV, HCV, Syphilis and Malaria). Panels should be tested how a blood unit is routinely screened to mimic the actual laboratory process. This allows the NRL and participant to check and validate the entire blood unit screening process.
Test results were analyzed by OASYS Canada using the ISO 13528:2005 Robust Statistics method (Huber’s Method) to identify outliers. Data analysis from the test event showed a significant number of participants that reported aberrant results due to errors related to random or systematic errors. This also showed deviations from standard practice recommended by the Department of Health as well as a comparison of different test platforms for blood screening.
Ultimately, the data gathered from the EQAS are used to improve on policies for blood screening and set recommendations for the safety of the Philippine blood supply.
Pupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
Authors: Persson Staffan , Zheng Shuai , Noack Lise , Khammy Ouda , De Meyer Andreas , Khan Ghazanfar , De Winne Nancy , Eeckhout Dominique , Damme Daniel Van 
Published: September 2024

Abstract

Knowledge about how and where proteins interact provides a pillar for cell biology. Protein proximity-labelling has emerged as an important tool to detect protein interactions. Here, biotin-related proximity labelling approaches are by far the most commonly used, but may have labelling-related drawbacks. Here, we use pupylation-based proximity labelling (PUP-IT) as a tool for protein interaction detection in plants. We show that PUP-IT readily confirmed protein interactions for several known protein complexes across different types of plant hosts and that the approach increased detection of specific interactions as compared to biotin-based proximity labelling systems. To further demonstrate the power of PUP-IT, we used the system to identify protein interactions of the protein complex that underpin cellulose synthesis in plants. Apart from known complex components, we identified the ARF-GEF BEN1 (BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE1). We show that BEN1 contributes to cellulose synthesis by regulating both clathrin-dependent and -independent endocytosis of the cellulose synthesis protein complex from the plasma membrane. Our results highlight PUP-IT as a powerful proximity labelling system to identify protein interactions in plant cells.
Effects of ephedrine on the nociception level and analgesia nociception index
Authors: Kuroki Masahiro , Onodera Yu , Okada Masayuki , Nakane Masaki 
Published: September 2024

Abstract

Nociceptive monitor values are influenced by vasopressors; however, no study has investigated this in detail. In this study, we aimed to investigate the effect of ephedrine on nociceptive monitors using the Analgesia Nociception Index (ANI) and Nociception Level (NOL). This prospective observational study was conducted between February and April 2023. Data were obtained during anesthesia induction only to exclude surgical invasion. Blood pressure, heart rate, ANI, and NOL were measured 5 min after ephedrine administration during hypotension. Low ANI and high NOL values indicated severe pain. Statistical analyses were performed on the values before and after ephedrine administration for each parameter. Eight patients (six men and two women) with a mean age of 72 years were included. The ANI and NOL, before versus after ephedrine administration were 76.3±11.8 versus 33.6±8.1 (p<0.001) and 2.1±1.5 versus 27.6±10.5 (p<0.001), respectively. The mean time beyond the pain threshold (ANI<50 and NOL>25) for ephedrine was 58.0 s for the ANI, and 29.3 s for the NOL, respectively. The administration of ephedrine, albeit for a short duration, significantly altered nociceptive monitor values, occasionally surpassing thresholds indicative of pain.
Direct observation of layer skyrmions in twisted WSe2 bilayers
Authors: Shih Chih-Kang , Zhang Fan , Morales-Durán Nicolás , Li Yanxing , Yao Wang , Su Jung-Jung , Lin Yu-Chuan , Dong Chengye , Liu Xiaohui , Chen Fu-Xiang , Kim Hyunsue , Watanabe Kenji , Taniguchi Takashi , Li Xiaoqin , Robinson Joshua , MacDonald Allan 
Published: September 2024

Abstract

Transition metal dichalcogenide (TMD) twisted homobilayers have been established as an ideal platform for studying strong correlation phenomena, as exemplified by the recent discovery of fractional Chern insulator (FCI) states in twisted MoTe21–4 and Chern insulators (CI)5 and unconventional superconductivity6,7 in twisted WSe2 (tWSe2). In these systems, nontrivial topology in the strongly layer-hybridized regime can arise from a spatial patterning of interlayer tunneling amplitudes and layer-dependent potentials that yields a lattice of layer skyrmions. Here we report the direct observation of skyrmion textures in the layer degree of freedom of Rhombohedral-stacked (R-stacked) tWSe2 homobilayers. This observation is based on scanning tunneling spectroscopy that separately resolves the G-valley and K-valley moiré electronic states. We show that G-valley states are subjected to a moiré potential with an amplitude of ~ 120 meV. At ~ 150 meV above the G-valley, the K-valley states are subjected to a weaker moiré potential of ~ 30 meV. Most significantly, we reveal opposite layer polarization of the K-valley at the MX and XM sites within the moiré unit cell, confirming the theoretically predicted layer skyrmion texture. The dI/dV mappings allow the parameters that enter the continuum model of moiré bands in twisted TMD bilayers to be determined experimentally, further establishing a direct correlation between the shape of the LDOS profile in real space and the topology of topmost moiré band.
Differences of pro-inflammatory and cell apoptosis markers between patients with normal and poor ovarian response during in vitro fertilization program Running Titel: Pro-inflammatory markers in IVF patients
Authors: Ruangprachum Gadsara , Thuwanut Paweena , Sereepapong Wisan , Shotelersuk Vorasuk , Tongkobpetch Siraprapa , Summat Sadanan , Sirayapiwat Porntip 
Published: September 2024

Abstract

This study aimed to compare pro-inflammatory (Interleukin-6; IL-6 and TNF-α) and cell apoptosis (GABRA-1) markers in patients with normal ovarian response (NOR) and poor ovarian response (POR) to ovarian stimulation during in vitro fertilization (IVF) treatment. A cross-sectional study was conducted with 27 patients (NOR = 16 and POR = 11). Pleiotropic cytokine IL-6 levels in follicular fluid were determined by enzyme-linked immunosorbent assay. Relative expression levels of TNF-α and GABRA-1 related genes were determined in cumulus cells by reverse transcription-quantitative polymerase chain reaction. TNF-α levels were significantly higher in POR group compared to NOR group (POR = 7.1 ± 1.9 and NOR = 2.4 ± 1.5, P < 0.001). Additionally, positive correlation was noted between TNF-α relative expression levels and factors associated with ovarian response, including levels of anti-müllerian hormone (AMH) (r2 = 0.69, P = 0.008), antral follicle count (r2 = 0.73, P < 0.001) and number of retrieved oocytes (r2 = 0.74, P < 0.001). However, IL-6 and GABRA-1 levels did not differ between cohorts. In conclusion, TNF-α presents significantly different between NOR and POR patients, suggesting its crucial role in ovarian response during IVF treatment. This finding provides an opportunity for further study of POR pathogenesis.
A 20-year follow-up study of patients who have been diagnosed with familial hypercholesterolemia in their youth
Authors: Widhalm Kurt , Fallmann Karin 
Published: September 2024

Abstract

Aim
Familial Hypercholesterolemia is an autosomal dominant inherited metabolic disorder characterized by elevated plasma levels of low-density lipoprotein cholesterol (LDL-C). These cause atherosclerosis, premature cardiovascular (cv) disease and death.
Despite high prevalence long-term data about FH patients is very limited. Therefore, this study presents data in regard to effects and outcomes of long-term treatment of FH-patients starting in childhood and adolescence.
Methods
A data collection of 13 FH patients was made for this follow up study. On average the FH diagnosis had been made 20 years ago (9 - 31 years). 86% of the included patients have a genetically confirmed FH diagnosis. The investigated parameters are family history, LDL-C at diagnosis, latest LDL-C, FH-causing mutations, therapy, compliance and if provided by the patient the intima media thickness of the carotid artery.
Results
The mean initial LDL-C at diagnosis was 277 mg/dl (494 mg/dl - 173 mg/dl). While the mean latest LDL-C was 112 mg/dl (208mg/dl - 70mg/dl). Equaling a mean LDL-C reduction of 59,6% from diagnosis to follow-up.
No clinical signs of manifestation of cv diseases could be observed. Furthermore, there have not been any reports of side effects of the lipid-lowering therapy.
Conclusions
The long-term treatment of patients with FH beginning in childhood is feasible and successful. As shown in this study a therapy with statins lowers LDL-C levels by more than half and therefore most probably prevents the occurrence of later occurring cardiovascular diseases.
Bactericidal, virucidal and fungicidal effect of ZnO, Ag, and ZnO+Ag coatings
Authors: Kacprzyńska-Gołacka Joanna , Wieciński Piotr , Stobnicka-Kupiec Agata , Górny Rafał , Krasiński Andrzej , Sowa Sylwia , Garbacz Halina , Smolik Jerzy , Gradoń Leon 
Published: September 2024

Abstract

Intensified globalization means the wider spread of pathogenic viruses, bacteria and fungi. Many such microorganisms are transmitted through contact with surfaces polluted with biodeposits as well as through human activity, which greatly increases the risk of infections caused by microbes. Therefore, the search for new effective solutions to inactivate pathogenic microorganisms that ensure safety and improve quality and comfort of life has become a global priority and major challenge. A very effective activity in this area may be the use of surface engineering technologies, which enable the production of coatings with biocidal properties. The very large possibilities of these technologies in terms of shaping the microstructure, chemical composition and phase composition of coatings enable effective shaping of functional properties. The aim of this study was to compare the properties of three types of coatings deposited by HIPIMS characterized by different chemical compositions and microstructures. In this work, all the produced coatings were analyzed in terms of their material, physical and biocidal properties. The authors confirmed the very good biocidal properties of the tested materials against various groups of microorganisms (bacteria, fungi and viruses) at the same time.
Antioxidant Activity and Phytochemical Constituents of Pawpaw (Carica Papaya) During Ripening
Authors: Brotoboh Kelvin , Ogunbiyi Oluwagbenga J. , Iyare Harrison E. , Amusan Temiloluwa O. 
Published: September 2024

Abstract

Carica papaya L. (Pawpaw) is one of the most commonly cultivated fruits in tropical and subtropical countries, especially in Nigeria. Nutritionally, Carica papaya is known to be highly nutritious. The effect of ripening on the antioxidant activity and phytochemical constituents (such as alkaloids, flavonoids, phenols, saponins, and tannins) of aqueous extract of two cultivars of Carica papaya (Local pawpaw and Agric pawpaw) was determined in this study. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to determine antioxidant activity, expressed as % DPPH scavenging activity. % DPPH scavenging activity significantly increased (p < 0.01) in both Carica papaya cultivars during ripening, with the overripe stage of both cultivars (Local pawpaw and Agric pawpaw) having the highest % DPPH scavenging activity. Phytochemical constituent analysis in Local pawpaw showed that alkaloids, flavonoids, phenols, and saponins increased during ripening with the overripe cultivar having the highest amount, while tannins decreased during ripening with the unripe having the highest amount. Agric pawpaw showed that alkaloids, flavonoids, and saponins increased during ripening with the overripe cultivar having the highest amount, while tannins and phenols decreased during ripening with the unripe cultivar having the highest amount. This result showed the important role of ripening stages in increasing the antioxidant activity of Carica papaya.