bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distance delivery of a parent-implemented language intervention for young boys with fragile X syndrome
Authors: Bullard Lauren , McDuffie Andrea , Abbeduto Leonard 
Published: September 2017

Background In addition to significant cognitive delays, boys with fragile X syndrome display phenotypic characteristics that include delays in language, inattention, social anxiety, and escape-maintained challenging behaviors. Despite these challenges, families affected by fragile X syndrome often have limited access to center-based intervention programs. Methods The present study utilized a multiple baseline design across participants to examine the preliminary effectiveness of a 12-week, parent-implemented spoken language intervention for three 5- to 7-year-old boys with fragile X syndrome. The goal of the intervention was to teach the biological mothers of each boy to use a set of verbally responsive language support strategies while participating in shared book reading activities with her child. All aspects of the intervention, including pre- and post-treatment measures, were delivered into the family’s home via distance video teleconferencing. Results Results from this study provide preliminary support for the efficacy of this parent-implemented language intervention approach by demonstrating generalized improvements in both targeted maternal strategy use and measures of child spoken language. Conclusions The present study expands upon prior language intervention research utilizing distance delivery of services for families affected by fragile X syndrome. Additionally, the study provides early support for the feasibility of collecting pre- and post-treatment assessments at a distance.
A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri
Authors: Baumgart Mario , Barth Emanuel , Savino Aurora , Groth Marco , Koch Philipp , Petzold Andreas , Arisi Ivan , Platzer Matthias , Marz Manja , Cellerino Alessandro 
Published: September 2017

Abstract
Background
The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age dependent control of gene expression.

Results
Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri, as compared to other teleosts, was a duplication of the miR-29 cluster.

Conclusion
The completeness of the catalogue we provide is comparable to that of the zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.
Axonal localization of neuritin/CPG15 mRNA is limited by competition for HuD binding
Authors: Gomes Cynthia , Lee Seung Joon , Gardiner Amy S. , Smith Terika , Sahoo Pabitra K. , Patel Priyanka , Thames Elizabeth , Rodriguez Reycel , Taylor Ross , Yoo Soonmoon , Heise Tilman , Kar Amar N. , Perrone-Bizzozero Nora , Twiss Jeffery L. 
Published: September 2017

ABSTRACT
HuD protein (also known as ELAVL4) has been shown to stabilize mRNAs with AU-rich elements (ARE) in their 3′ untranslated regions (UTRs), including Gap43, which has been linked to axon growth. HuD also binds to neuritin (Nrn1) mRNA, whose 3′UTR contains ARE sequences. Although the Nrn1 3′UTR has been shown to mediate its axonal localization in embryonic hippocampal neurons, it is not active in adult dorsal root ganglion (DRG) neurons. Here, we asked why the 3′UTR is not sufficient to mediate the axonal localization of Nrn1 mRNA in DRG neurons. HuD overexpression increases the ability of the Nrn1 3′UTR to mediate axonal localizing in DRG neurons. HuD binds directly to the Nrn1 ARE with about a two-fold higher affinity than to the Gap43 ARE. Although the Nrn1 ARE can displace the Gap43 ARE from HuD binding, HuD binds to the full 3′UTR of Gap43 with higher affinity, such that higher levels of Nrn1 are needed to displace the Gap43 3′UTR. The Nrn1 3′UTR can mediate a higher level of axonal localization when endogenous Gap43 is depleted from DRG neurons. Taken together, our data indicate that endogenous Nrn1 and Gap43 mRNAs compete for binding to HuD for their axonal localization and activity of the Nrn1 3′UTR.
An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1β
Authors: Brough David , Pelegrin Pablo , Nickel Walter 
Published: September 2017

ABSTRACT
Extracellular proteins with important signalling roles in processes, such as inflammation and angiogenesis, are known to employ unconventional routes of protein secretion. Although mechanisms of unconventional protein secretion are beginning to emerge, the precise molecular details have remained elusive for the majority of cargo proteins secreted by unconventional means. Recent findings suggest that for two examples of unconventionally secreted proteins, interleukin 1β (IL-1β) and fibroblast growth factor 2 (FGF2), the common molecular principle of pore formation may be shared. Under specific experimental conditions, secretion of IL-1β and FGF2 is triggered by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent formation of pores across the plasma membrane. However, the underlying mechanisms are different, with FGF2 known to directly interact with PI(4,5)P2, whereas in the case of IL-1β secretion, it is proposed that the N-terminal fragment of gasdermin D interacts with PI(4,5)P2 to form the pore. Thus, although implemented in different ways, these findings suggest that pore formation may be shared by the unconventional secretion mechanisms for FGF2 and IL-1β in at least some cases. In this Opinion article, we discuss the unconventional mechanisms of FGF2 and IL-1β release with a particular emphasis on recent discoveries suggesting the importance of pore formation on the plasma membrane.
LPP3 mediates self-generation of chemotactic LPA gradients by melanoma cells
Authors: Susanto Olivia , Koh Yvette W. H. , Morrice Nick , Tumanov Sergey , Thomason Peter A. , Nielson Matthew , Tweedy Luke , Muinonen-Martin Andrew J. , Kamphorst Jurre J. , Mackay Gillian M. , Insall Robert H. 
Published: September 2017

ABSTRACT
Melanoma cells steer out of tumours using self-generated lysophosphatidic acid (LPA) gradients. The cells break down LPA, which is present at high levels around the tumours, creating a dynamic gradient that is low in the tumour and high outside. They then migrate up this gradient, creating a complex and evolving outward chemotactic stimulus. Here, we introduce a new assay for self-generated chemotaxis, and show that raising LPA levels causes a delay in migration rather than loss of chemotactic efficiency. Knockdown of the lipid phosphatase LPP3 – but not of its homologues LPP1 or LPP2 – diminishes the cell's ability to break down LPA. This is specific for chemotactically active LPAs, such as the 18:1 and 20:4 species. Inhibition of autotaxin-mediated LPA production does not diminish outward chemotaxis, but loss of LPP3-mediated LPA breakdown blocks it. Similarly, in both 2D and 3D invasion assays, knockdown of LPP3 diminishes the ability of melanoma cells to invade. Our results demonstrate that LPP3 is the key enzyme in the breakdown of LPA by melanoma cells, and confirm the importance of attractant breakdown in LPA-mediated cell steering.
This article has an associated First Person interview with the first author of the paper.
Single-Molecule Analysis Methods Using Nanogap Electrodes and Their Application to DNA Sequencing Technologies
Authors: Taniguchi Masateru 
Published: September 2017

Abstract
Single-molecule analysis methods facilitate the investigation of the properties of single-molecule junctions (SMJs), in which single molecules are connected between a pair of nanoelectrodes that use nanogap electrodes having a spacing of less than several nanometers. Various methods have been developed to investigate numerous useful parameters for SMJs; for example, the number of molecules connected between a pair of nanoelectrodes can be determined, the types and structures of single molecules can be revealed, localized temperatures within SMJs can be evaluated, and the Seebeck coefficient and the bond strength between single molecules and electrodes can be ascertained. Single-molecule analysis methods have also been used to analyze biopolymers in solutions, and this has resulted in single-molecule sequencing technologies being developed that can determine sequences of base molecules in DNA and RNA along with sequences of amino acids in peptides. Single-molecule analysis methods are expected to develop into digital analysis techniques that can be used to investigate the physical and chemical properties of molecules at single-molecule resolutions.
Supporting evidence-based analysis for modified risk tobacco products through a toxicology data-sharing infrastructure
Authors: Boué Stéphanie , Exner Thomas , Ghosh Samik , Belcastro Vincenzo , Dokler Joh , Page David , Boda Akash , Bonjour Filipe , Hardy Barry , Vanscheeuwijck Patrick , Hoeng Julia , Peitsch Manuel 
Published: September 2017

The US FDA defines modified risk tobacco products (MRTPs) as products that aim to reduce harm or the risk of tobacco-related disease associated with commercially marketed tobacco products. Establishing a product’s potential as an MRTP requires scientific substantiation including toxicity studies and measures of disease risk relative to those of cigarette smoking. Best practices encourage verification of the data from such studies through sharing and open standards. Building on the experience gained from the OpenTox project, a proof-of-concept database and website (INTERVALS) has been developed to share results from both in vivo inhalation studies and in vitro studies conducted by Philip Morris International R&D to assess candidate MRTPs. As datasets are often generated by diverse methods and standards, they need to be traceable, curated, and the methods used well described so that knowledge can be gained using data science principles and tools. The data-management framework described here accounts for the latest standards of data sharing and research reproducibility. Curated data and methods descriptions have been prepared in ISA-Tab format and stored in a database accessible via a search portal on the INTERVALS website. The portal allows users to browse the data by study or mechanism (e.g., inflammation, oxidative stress) and obtain information relevant to study design, methods, and the most important results. Given the successful development of the initial infrastructure, the goal is to grow this initiative and establish a public repository for 21st-century preclinical systems toxicology MRTP assessment data and results that supports open data principles.
The unhappy postdoc: a survey based study
Authors: Grinstein Amir , Treister Roi 
Published: September 2017

Background: The emerging public discourse about the “broken” postdoc system is mostly conceptual. The current work offers an attempt to quantify postdocs’ perceptions, goals, and well-being. Methods: A survey of 190 postdocs in North America. Results: This article first reveals a surprisingly unhappy postdoc community with low satisfaction with life scores. Second, it demonstrates how over the course of the fellowship many postdocs lose interest in the goal of pursuing a tenure track academic position (~20%) or in recommending the postdoc track to others (~30%). Finally, we find that among a large number of factors that can enhance satisfaction with life for postdocs (e.g., publication productivity, resources available to them) only one factor stood out as significant: the degree to which atmosphere in the lab is pleasant and collegial. Conclusions: Our findings can stimulate policy, managerial, and career development improvements in the context of the postdoc system.
Geographic-genetic analysis of Plasmodium falciparum parasite populations from surveys of primary school children in Western Kenya
Authors: Omedo Irene , Mogeni Polycarp , Rockett Kirk , Kamau Alice , Hubbart Christina , Jeffreys Anna , Ochola-Oyier Lynette Isabella , de Villiers Etienne P. , Gitonga Caroline W. , Noor Abdisalan M. , Snow Robert W. , Kwiatkowski Dominic , Bejon Philip 
Published: September 2017

Background. Malaria control, and finally malaria elimination, requires the identification and targeting of residual foci or hotspots of transmission. However, the level of parasite mixing within and between geographical locations is likely to impact the effectiveness and durability of control interventions and thus should be taken into consideration when developing control programs. Methods. In order to determine the geographic-genetic patterns of Plasmodium falciparum parasite populations at a sub-national level in Kenya, we used the Sequenom platform to genotype 111 genome-wide distributed single nucleotide polymorphic (SNP) positions in 2486 isolates collected from children in 95 primary schools in western Kenya. We analysed these parasite genotypes for genetic structure using principal component analysis and assessed local and global clustering using statistical measures of spatial autocorrelation. We further examined the region for spatial barriers to parasite movement as well as directionality in the patterns of parasite movement. Results. We found no evidence of population structure and little evidence of spatial autocorrelation of parasite genotypes (correlation coefficients <0.03 among parasite pairs in distance classes of 1km, 2km and 5km; p value<0.01). An analysis of the geographical distribution of allele frequencies showed weak evidence of variation in distribution of alleles, with clusters representing a higher than expected number of samples with the major allele being identified for 5 SNPs. Furthermore, we found no evidence of the existence of spatial barriers to parasite movement within the region, but observed directional movement of parasites among schools in two separate sections of the region studied. Conclusions. Our findings illustrate a pattern of high parasite mixing within the study region. If this mixing is due to rapid gene flow, then “one-off” targeted interventions may not be currently effective at the sub-national scale in Western Kenya, due to the high parasite movement that is likely to lead to re-introduction of infection from surrounding regions. However repeated targeted interventions may reduce transmission in the surrounding regions.
TeenBP: Development and Piloting of an EHR-linked Clinical Decision Support System
to Improve Recognition of Hypertension in Adolescents
Authors: Kharbanda Elyse O. , Nordin James D. , Sinaiko Alan R. , Ekstrom Heidi L. , Stultz Jerry M. , Sherwood Nancy E. , Fontaine Patricia L. , Asche Steve E. , Dehmer Steven P. , Amundson Jerry H. , Appana Deepika X. , Bergdall Anna R. , Hayes Marcia G. , O'Connor Patrick J. 
Published: September 2017

Context: Blood pressure (BP) is routinely measured in children and adolescents
during primary care visits. However, elevated BP or hypertension is frequently not
diagnosed or evaluated further by primary care providers. Barriers to recognition
include lack of clinician buy-in, competing priorities, and complexity of the standard
BP tables.Case Description: We have developed and piloted TeenBP— a web-based,
electronic health record (EHR) linked system designed to improve recognition of
prehypertension and hypertension in adolescents during primary care visits.Major
Themes: Important steps in developing TeenBP included the following: review of national
BP guidelines, consideration of clinic workflow, engagement of clinical leaders, and
evaluation of the impact on clinical sites. Use of a web-based platform has facilitated
updates to the TeenBP algorithm and to the message content. In addition, the web-based
platform has allowed for development of a sophisticated display of patient-specific
information at the point of care. In the TeenBP pilot, conducted at a single pediatric
and family practice site with six clinicians, over a five-month period, more than half
of BPs in the hypertensive range were clinically recognized. Furthermore, in this small
pilot the TeenBP clinical decision support (CDS) was accepted by providers and clinical
staff. Effectiveness of the TeenBP CDS will be determined in a two-year
cluster-randomized clinical trial, currently underway at 20 primary care
sites.Conclusion: Use of technology to extract and display clinically relevant data
stored within the EHR may be a useful tool for improving recognition of adolescent
hypertension during busy primary care visits. In the future, the methods developed
specifically for TeenBP are likely to be translatable to a wide range of acute and
chronic issues affecting children and adolescents.
‘Black Duck Feathers’ or becoming-perfect
Authors: Bucci Alessandro 
Published: September 2017

Abstract
This article offers a study of one singular dress created by fashion designer Lee Alexander McQueen, namely, the ‘Black Duck Feathers’. Consisting of preternaturally oversized shoulders and hips and of black feathers covering the entire figure, this specific dress emblematically served to turn an indistinct female body into a romantic, yet melancholic black bird of prey. While ‘Black Duck Feathers’ has been interpreted within the frame of Romanticism in exhibitions at the MET and the V&A, this article makes the dress the starting point of a dialogue between Alexander McQueen’s creative process and Deleuze and Guattari’s complex philosophical theory of becoming, which they develop in A Thousand Plateaus: Capitalism and Schizophrenia. The aim is to highlight the possibility for an artefact to be a tool for illuminating philosophical theories and concepts and to be the trigger of innumerable and complex considerations. This is in contrast to the way in which critical theory is traditionally used, which is to say, to analyse phenomena, cultural processes and expressions of material culture. The choice of making theory the real object of study in this article is inspired by the very idea of metamorphosis and openness of the body that shapes ‘Black Duck Feathers’ and that seems to characterize Alexander McQueen’s poetics. Metamorphosis of the body, of fields and of theories can be used to study fashion, and is understood here as a way of playing on the edge and among frontiers and as a desire and a need to exceed their borders.
Dress, body and experiences of victimization
Authors: Reilly Andrew , Johnson Kim K. P. 
Published: September 2017

Abstract
Our research purpose was to uncover perceived relationships between aspects of appearance (i.e., body, dress) and experiences of any form of victimization from the perspective of survivors. We addressed three research questions: (1) what connection, if any, did survivors draw between their appearance and their experience of victimization?; (2) what changes, if any, did survivors make to their appearance after their experience(s)?; and (3) what advice, if any, would survivors give to others on appearance as a result of their experience? Five women and three men completed interviews. Participants identified appearance cues as a stimulus evoking others’ behaviours towards them. Both general appearance attributes and specific attributes were credited with eliciting negative behaviours. Experiences with victimization often occurred when the individual was attempting to move into a culture that was new to them. Most participants altered or made adjustments to their appearance as a result of their victimization experience.
It’s Ok to Fall
Authors: Iohe Taey 
Published: September 2017

Abstract
This piece of art writing imagines gravity and falling as quotidian experiences. There are two main characters: Muni (the name can be translated as Patterns, from the Korean original) and Garu (meaning Dust in Korean). Muni is undertaking a journey to a zero-gravity training facility in the small town of Shankill, Ireland; Garu works as a community-based psychologist. I chart her investigation of falling by taking a series of photographs and creating an installation. The installation is a room dusted with white powder, in which is hand-written a paragraph from a novel. This piece of art writing was exhibited as Language but No Words at the Space Mom Museum in Chungju, Korea in 2012.
Internet using status and perceived stress level of university students<p>Üniversite öğrencilerinin internet kullanım durumları ve algıladıkları stres düzeyleri
Authors: Işık Çalışkan Zehra , Caner Nuray , Evgin Derya , Kaplan Bahriye 
Published: September 2017

Abstract Objective: Internet and online technologies as well as present opportunities that make easy the life, they could bring some negativity. This study was conducted to determine using internet statutes of university students at a Health College and stress levels that they perceived, as descriptive. Method: The study was conducted with 296 students in 2013-2014 education session by taking consent from the institute and students. Data was collected by Individual Information From and Perceived Stress Scale. Results: It is determined that of the students; 37.3% spent time with internet 4 hours and more daily, and of these students; 70.3% had got stress as moderate level, with increase in staying time at internet environment, moderate and high level stress increased (p<0.05). Also, academic success level decreased with increase in staying time at internet but there wasn’t any significant difference between them (p>0.05). Conclusion: It is seen that staying time at internet could affect the perceived stress level. It is suggested that the students should be given education and counseling about more conscient using of internet and effective coping strategies with stress.Extended English abstract is in the end of PDF (TURKISH) file.ÖzetAmaç: İnternet ve online teknolojiler yaşamı kolaylaştıracak pek çok imkan sunarken, beraberinde bazı olumsuzlukları da getirmiştir. Bu çalışma, bir üniversitenin Sağlık Yüksekokulu öğrencilerinin internet kullanım durumları ve algıladıkları stres düzeylerinin belirlenmesi amacıyla tanımlayıcı olarak yapılmıştır. Yöntem: Çalışma, 2013-2014 eğitim öğretim döneminde, kurum ve öğrenci onamı alınarak, 296 öğrenciyle yürütülmüştür. Veriler, Kişisel Bilgi Formu ve Algılanan Stres Ölçeği ile toplanmıştır. Bulgular: Öğrencilerin %37.3’ünün, günlük 4 saat ve daha fazla süreyle internete bağlandığı, günlük 4 saat ve daha fazla süreyle internete bağlı kalan öğrencilerin %70.3’ünün orta stres düzeyinde olduğu, internette kalma süresi arttıkça öğrencilerin orta ve yüksek stres düzeyi oranlarında artış olduğu belirlenmiştir (p<0.05). Ayrıca öğrencilerin internette kalma süreleri arttıkça akademik başarı oranlarının düştüğü ancak farkın önemli olmadığı bulunmuştur (p>0.05). Sonuç:İnternette kalma süresinin algılanan stres düzeyini etkileyebileceği sonucuna ulaşılmıştır. Öğrencilere; internet ve online teknolojileri daha bilinçli kullanma ve stresle etkin başedebilme yollarıyla ilgili eğitim ve danışmanlık verilmesi önerilmektedir.// // Annotate Highlight