bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of KIR-genotypes in women with preimplantation losses after assisted reproductive technologies
Authors: Sosnina K. O. , Zastavna D. V. , Tretiak B. I. , Terpylyak O. I. 
Published: October 2024

Aim. To analyze the frequency and distribution of KIR genotypes in women with repeated preimplantation losses during ARTs compared to women with control group. Methods. DNA isolation from leukocytes by the salting method, PCR-SSP, agarose gel electrophoresis; methods of statistical analysis. Results. The molecular genetic determination of KIR genes in the group of women with recurrent implantation failures and in the control group of women was performed. The frequency and distribution of KIR genotypes in the study groups were determined. In women with RIF, a slightly shifted distribution of the frequency of KIR genotypes was found, towards the homozygous genotype by haplotype "A", the frequency of which was almost half of all identified genotypes (42.86 %). The distribution and frequency of KIR genotypes in the control group was normal and described by the Hardy-Weinberg principle. A comparative analysis of the study groups using statistical methods showed a significantly higher frequency of the KIR-AA genotype (χ2=8.875; p<0.005) in the group of women with RIF compared to the control group of women. The calculation of the odds ratio indicates a 2-fold increase of risk for recurrent implantation failures after ARTs (OR=1.94; CI 95 %: 1.25–3.00) in women with the KIR-AA genotype. Conclusions. We regard the KIR-AA genotype as a significant risk factor for preimplantation loss. KIR genotyping is considered an important molecular genetic test for determining the risk of fetal rejection by the maternal immune system, which will help to correctly direct treatment interventions to keep the pregnancy alive.
The effect of post-activation enhancement on the performance of Chinese national skeleton athletes in the “ice push sled”-a first cross-sectional study
Authors: Tian Guang , Li Haojie , Zhu Huan , Gao Binghong 
Published: October 2024

Objective
To investigate the impact of post-activation potentiation (PAP) induced by resisted sled sprint at different loads on the subsequent 30 m ice push sled performance of Chinese skeleton athletes, and to identify the resisted sled sprint load that most effectively enhances PAP for Chinese skeleton athletes.

Methods
Seven elite athletes from the Chinese skeleton team participated in four tests with more than 48 h intervals. During the tests, on the first test, athletes completed a 40 min standard warm-up, rested for 6 min, and then performed a 30 m test. On the second, third, and fourth test, athletes completed the standard warm-up, then performed 20 m sprints with resisted sled (RS) at 75%, 50%, and 25% of body mass (BM), respectively, rested for 6 min, and then performed the 30 m test.

Results
No significant differences were found in morning pulse, blood urea, and creatine kinase levels among four tests. The percentage of maximum heart rate (%HRmax) within different intensity ranges showed no significant differences among four tests. However, significant differences were observed in ice push sled performance among four tests (No BMRS: 5.08 ± 0.27; 25% BMRS: 5.05 ± 0.29; 50% BMRS: 5.02 ± 0.27; 75% BMRS: 5.04 ± 0.28). Post hoc analyses revealed that the 50% BMRS test had faster speed compared to the no resistance (p < 0.05), the 25% BMRS (p < 0.05), and the 75% BMRS (p < 0.05) tests. Additionally, the 75% BMRS test had faster speed than the no resistance test (p < 0.05).

Conclusion
A 20 m sprint with 50% BMRS effectively enhances the PAP effect in skeleton athletes, improving their ice push sled performance. Coaches can incorporate this resisted sled sprint in athletes’ training routines for performance enhancement in both daily training and pre-competition preparations.
Advancing Prostate Cancer Diagnosis and Treatment Through AI-Driven Decision-making: A Comprehensive PRISMA-Based Systematic Review
Authors: Choudhary Rohit , Swain Santi Swarup , Khairari Anshul , Thatoi Priyabrata , Thatoi Hrudayanath 
Published: October 2024

Decision-making during the diagnosis and treatment of prostate cancer often requires the evaluation of data from several sources. The purpose of this systematic was to evaluate how AI can enhance prostate cancer diagnosis and treatment. We search five different databases for available studies using a pre-specified search strategy. The study was conducted using PRISMA guidelines. A total of 1058 studies were found on five different databases (Scopus N=287, Web of Science N=204, PubMed/EMBASE N=306, Cochrane Library N=94, Google Scholar N=167). The articles were stored in the ENDNOTE library and all the duplicates were removed. We found 19 relevant studies that were included after a stringent assessment criterion based on the inclusion and exclusion criteria. Included studies were assessed for risk bias assessment using the Newcastle Ottawa Scale (NOS) in which one study was found to have high-risk bias assessment, seven were identified to have low-risk bias assessment while the rest of the studies were moderate. Clinicians can recognize complex correlations and handle massive data sets with the help of artificial intelligence. These tasks are extremely laborious and challenging for humans to complete. It is feasible to employ fewer resources while increasing overall effectiveness and precision in prostate cancer detection and treatment by utilizing AI algorithms and lowering the degree of subjectivity. This review provides a comprehensive comparison of AI algorithms in detection of prostate cancer.
Investigating the Public Awareness and Utilization of Health Screening Services Offered by Community Pharmacies
Authors: Ch Mahnoor , Zaman Minahil , Rauf Samra Abdul , Mairaj Zaki Ullah , Jamil Laiba , Essa Samra , Chaudhary Noor-e-Hizba , Mehr-un-nisa  , Riaz Javeria , Iqbal Muhammad Zahid 
Published: October 2024

Background: Community pharmacies are the integral part of the healthcare system offering healthcare services for patients. Pharmacists are expanding their roles by providing pharmaceutical services and comprehensive pharmaceutical care. Health screenings played an important role in early detection of diseases and potential risk factors.
Objectives: To assess the extent of awareness, utilization and impact of using health screening services offered by community pharmacy among public and to identify the barriers to access these services.
Methods: A cross-sectional study was conducted in different cities of Punjab, Pakistan. Data was collected using validated questionnaire after getting consent from general public. The sample size for this study was 151. SPSS version 26th was used as a statistical tool to analyze data. P-value < 0.05 were considered as statistically significant.
Results: Health screening services were more used by middle age individuals and adults. Individuals holding bachelor’s degree (41.2%) and female respondents (42.9%) showed more satisfactory response than male respondents. Blood pressure screenings (53.6%), diabetes screenings (28.5%) and other services (34.4%) were mostly used by the participants Barriers to health screening services include lack of awareness, lack of time and preference to visit doctor.
Conclusion: Screening services offered at community pharmacies are found satisfactory, convenient and economical by most people. However utilization of these services is less frequent indicating a need for improved awareness and public education about the services offered by community pharmacy. Attention should be given to reduce barriers to the use of health screening services.
Regional influences on reconstructed global mean sea level
Authors: Natarov Svetlana I. , Merrifield Mark A. , Becker Janet M. , Thompson Phillip R. 
Published: March 2017

AbstractReconstructions of global mean sea level (GMSL) based on tide gauge measurements tend to exhibit common multidecadal rate fluctuations over the twentieth century. GMSL rate changes may result from physical drivers, such as changes in radiative forcing or land water storage. Alternatively, these fluctuations may represent artifacts due to sampling limitations inherent in the historical tide gauge network. In particular, a high percentage of tide gauges used in reconstructions, especially prior to the 1950s, are from Europe and North America in the North Atlantic region. Here a GMSL reconstruction based on the reduced space optimal interpolation algorithm is deconstructed, with the contributions of individual tide gauge stations quantified and assessed regionally. It is demonstrated that the North Atlantic region has a disproportionate influence on reconstructed GMSL rate fluctuations prior to the 1950s, notably accounting for a rate minimum in the 1920s and contributing to a rate maximum in the 1950s. North Atlantic coastal sea level fluctuations related to wind‐driven ocean volume redistribution likely contribute to these estimated GMSL rate inflections. The findings support previous claims that multidecadal rate changes in GMSL reconstructions are likely related to the geographic distribution of tide gauge stations within a sparse global network.
Revised cloud processes to improve the mean and intraseasonal variability of Indian summer monsoon in climate forecast system: Part 1
Authors: Abhik S. , Krishna R. P. M. , Mahakur M. , Ganai Malay , Mukhopadhyay P. , Dudhia J. 
Published: March 2017

AbstractThe National Centre for Environmental Prediction (NCEP) Climate Forecast System (CFS) is being used for operational monsoon prediction over the Indian region. Recent studies indicate that the moist convective process in CFS is one of the major sources of uncertainty in monsoon predictions. In this study, the existing simple cloud microphysics of CFS is replaced by the six‐class Weather Research Forecasting (WRF) single moment (WSM6) microphysical scheme. Additionally, a revised convective parameterization is employed to improve the performance of the model in simulating the boreal summer mean climate and intraseasonal variability over the Indian summer monsoon (ISM) region. The revised version of the model (CFSCR) exhibits a potential to improve shortcomings in the seasonal mean precipitation distribution relative to the standard CFS (CTRL), especially over the ISM region. Consistently, notable improvements are also evident in other observed ISM characteristics. These improvements are found to be associated with a better simulation of spatial and vertical distributions of cloud hydrometeors in CFSCR. A reasonable representation of the subgrid‐scale convective parameterization along with cloud hydrometeors helps to improve the convective and large‐scale precipitation distribution in the model. As a consequence, the simulated low‐frequency boreal summer intraseasonal oscillation (BSISO) exhibits realistic propagation and the observed northwest‐southeast rainband is well reproduced in CFSCR. Additionally, both the high and low‐frequency BSISOs are better captured in CFSCR. The improvement of low and high‐frequency BSISOs in CFSCR is shown to be related to a realistic phase relationship of clouds.
QBO/solar modulation of the boreal winter Madden‐Julian oscillation: A prediction for the coming solar minimum
Authors: Hood Lon L. 
Published: March 2017

AbstractThe Madden‐Julian oscillation (MJO), also known as the 30–60 day oscillation, is the strongest of the intraseasonal climate oscillations in the tropics and has significant derivative effects on extratropical circulation and intraseasonal climate. It has recently been shown that the stratospheric quasi‐biennial oscillation (QBO) modulates the amplitude of the boreal winter MJO such that MJO amplitudes are larger on average during the easterly phase (QBOE) than during the westerly phase (QBOW). A major possible mechanism is the decrease in static stability in the lowermost stratosphere under QBOE conditions resulting from relative upwelling associated with the QBO‐induced meridional circulation. Here evidence is presented that tropical upwelling changes related to the 11 year solar cycle also modulate the boreal winter MJO. Based on 37.3 years of MJO amplitude data, the largest amplitudes and occurrence rates, and the weakest static stabilities in the tropical lower stratosphere, occur during the QBOE phase under solar minimum (SMIN) conditions while the smallest amplitudes and strongest static stabilities occur during the QBOW phase under solar maximum (SMAX) conditions. Conversely, when the QBO and solar forcings are opposed (QBOW/SMIN and QBOE/SMAX), the difference in occurrence rates becomes statistically insignificant. During the coming solar minimum, at least one additional winter in the QBOE/SMIN category should occur (possibly as early as 2017/2018) during which especially large MJO amplitudes are expected and an initial test of these results will be possible.
Ensemble‐based Reconstructed Forcing (ERF) for regional climate modeling: Attaining the performance at a fraction of cost
Authors: Erfanian Amir , Wang Guiling , Fomenko Lori , Yu Miao 
Published: March 2017

AbstractMultimodel ensemble (MME) is considered the most reliable for both present‐day and future climate simulations. However, the conventional MME approach can create physical inconsistencies between climate variables. It can also be computationally inhibiting when using regional climate models (RCMs) due to uncertainties originating from both RCMs and the driving global climate models (GCMs) which must be accounted for. Here we propose the Ensemble‐based Reconstructed Forcing (ERF) approach which derives the RCM's initial and boundary conditions from the ensemble average of multiple GCMs. Using ensembles of up to six GCMs as examples, we show that the ERF approach with a single RCM integration performs comparably to the MME average in bias reduction. The ERF simulation provides unaltered solutions to the RCM's underlying equations, making it theoretically superior to the conventional MME approach which averages multiple solutions and may result in a physically implausible climate state.
A phase 1 study of AMG 900, an orally administered pan‐aurora kinase inhibitor, in adult patients with acute myeloid leukemia
Authors: Kantarjian Hagop M. , Schuster Michael W. , Jain Nitin , Advani Anjali , Jabbour Elias , Gamelin Erick , Rasmussen Erik , Juan Gloria , Anderson Abraham , Chow Vincent F. , Friberg Gregory , Vogl Florian D. , Sekeres Mikkael A. 
Published: March 2017

AbstractAurora kinases are involved in the pathophysiology of several cancers including acute myeloid leukemia (AML). In this phase 1 study, we investigated the safety and efficacy of AMG 900, an orally administered, highly potent, selective, small‐molecule inhibitor of both Aurora kinase A and B, in patients with AML . Patients with pathologically documented AML who either declined standard treatments or had relapsed from or were refractory to previous therapies were enrolled. Two every‐2‐week dose‐escalation schedules using a modified 3 + 3 + 3 design were evaluated AMG 900 given daily for 4 days with 10 days off (4/10 schedule), and AMG 900 given daily for 7 days with 7 days off (7/7 schedule). Thirty‐five patients were enrolled at 9 different dose levels: 22 patients on the 4/10 schedule (doses from 15 to 100 mg daily), and 13 patients on the 7/7 schedule (doses from 30 to 50 mg daily). Both schedules were tolerated; nausea (31%), diarrhea (29%), febrile neutropenia (29%), and fatigue (23%) were the most common treatment‐related adverse events. Three patients (9%) achieved complete response with incomplete count recovery. Patients with higher baseline expression of a set of specific pathway‐related genes (BIRC5, AURKA, TTK, CDC2, and CCNB1) were more likely to respond in an exploratory biomarker analysis. AMG 900 was tolerated in a general AML population, and pathway‐specific biomarkers identified a potential target population. Future research efforts will be directed toward further exploration of biomarkers of response and combination of AMG 900 with other anticancer agents.
Monocytosis in polycythemia vera: Clinical and molecular correlates
Authors: Barraco Daniela , Cerquozzi Sonia , Gangat Naseema , Patnaik Mrinal M. , Lasho Terra , Finke Christy , Hanson Curtis A. , Ketterling Rhett P. , Pardanani Animesh , Tefferi Ayalew 
Published: March 2017

AbstractMonocytosis (absolute monocyte count, AMC ≥ 1 × 109/L) might accompany a spectrum of myeloid neoplasms, other than chronic myelomonocytic leukemia (CMML). In the current study, we examined the prevalence, laboratory and molecular correlates, and prognostic relevance of monocytosis in polycythemia vera (PV). Among 267 consecutive patients with World Health Organization (WHO)‐defined PV, 55 (21%) patients displayed an AMC of ≥1 × 109/L and 18 (7%) an AMC of ≥1.5 × 109/L. In general, PV patients with monocytosis were significantly older and displayed higher frequencies of leukocytosis (81% vs. 50% at AMC ≥1 × 109/L) and TET2/SRSF2 mutations (57%/29% vs. 19%/1% at AMC ≥ 1.5 × 109/L). In univariate analysis, AMC ≥1.5 × 109/L adversely affected overall (OS; P = .004; HR 2.6, 95% CI 1.4‐4.8) and myelofibrosis‐free (MFFS; P = .02; HR 4.4, 95% CI 1.3‐15.1) survival; during multivariable analysis, significance was borderline sustained for OS (P = .05) and MFFS (P = .06). Other independent risk factors for OS included unfavorable karyotype (P = .02, HR 3.39, 95% CI 1.17‐9.79), older age (P < .0001, HR 3.34 95% CI 1.97‐5.65), and leukocytosis ≥15 × 109/L (P = .004, HR 2.04, 95% CI 1.26‐3.29). In conclusion, in the current study, we encountered a higher than expected prevalence of monocytosis in patients with PV and the mutation profile and age distribution of PV patients with monocytosis is akin to those of patients with CMML and might partly contribute to their worse prognosis.
Chiral Tertiary Sulfonium Salts as Effective Catalysts for Asymmetric Base‐Free Neutral Phase‐Transfer Reactions
Authors: Liu Shiyao , Maruoka Keiji , Shirakawa Seiji 
Published: March 2017

AbstractAlthough chiral quaternary ammonium and phosphonium salts are commonly used for asymmetric organocatalysis, the catalytic ability of chiral tertiary sulfonium salts has yet to be demonstrated in asymmetric synthesis. Herein, we show that chiral bifunctional trialkylsulfonium salts catalyze highly enantioselective conjugate additions of 3‐substituted oxindoles to maleimides under base‐free neutral phase‐transfer conditions.
Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi
Authors: Yang Yan‐long , Zhang Shasha , Ma Ke , Xu Yuxing , Tao Qiaoqiao , Chen Yihua , Chen Juan , Guo Shunxing , Ren Jinwei , Wang Wenzhao , Tao Yong , Yin Wen‐Bing , Liu Hongwei 
Published: March 2017

AbstractDiterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA‐related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily.
A Photocatalytically Active Lubricant‐Impregnated Surface
Authors: Wooh Sanghyuk , Butt Hans‐Jürgen 
Published: March 2017

AbstractLubricant impregnated surfaces (LISs) exhibit sliding angles below 5°. A LIS is presented that possesses photocatalytic activity as well as improved liquid repellency. In a single‐step reaction, the surface of photocatalytic mesoporous TiO2 substrate is modified by grafting polydimethylsiloxane (PDMS) brush and the residual non‐bound PDMS serves as lubricant. Since the lubricant and the hydrophobic layer are chemically identical, the grafting PDMS layer is stably swollen by the lubricant PDMS, which inhibits direct contact of liquid drops to the solid substrate. Liquid drops such as water, methanol, and even low‐surface‐tension fluorocarbons, slide on the surface with tilt angles below 1°. The surface exhibits long‐term stable photocatalytic activity while retaining its liquid repellency. This photocatalytic activity allows photocatalytic chemistry, for example, decomposition of organics, on LIS to be carried out.
Charges Shift Protonation: Neutron Diffraction Reveals that Aniline and 2‐Aminopyridine Become Protonated Upon Binding to Trypsin
Authors: Schiebel Johannes , Gaspari Roberto , Sandner Anna , Ngo Khang , Gerber Hans‐Dieter , Cavalli Andrea , Ostermann Andreas , Heine Andreas , Klebe Gerhard 
Published: March 2017

AbstractHydrogen atoms play a key role in protein–ligand recognition. They determine the quality of established H‐bonding networks and define the protonation of bound ligands. Structural visualization of H atoms by X‐ray crystallography is rarely possible. We used neutron diffraction to determine the positions of the hydrogen atoms in the ligands aniline and 2‐aminopyridine bound to the archetypical serine protease trypsin. The resulting structures show the best resolution so far achieved for proteins larger than 100 residues and allow an accurate description of the protonation states and interactions with nearby water molecules. Despite its low pKa of 4.6 and a large distance of 3.6 Å to the charged Asp189 at the bottom of the S1 pocket, the amino group of aniline becomes protonated, whereas in 2‐aminopyridine, the pyridine nitrogen picks up the proton although its amino group is 1.6 Å closer to Asp189. Therefore, apart from charge–charge distances, tautomer stability is decisive for the resulting binding poses, an aspect that is pivotal for predicting correct binding.
A Membrane‐Intercalating Conjugated Oligoelectrolyte with High‐Efficiency Photodynamic Antimicrobial Activity
Authors: Wang Bing , Wang Ming , Mikhailovsky Alexander , Wang Shu , Bazan Guillermo C. 
Published: March 2017

AbstractA membrane‐intercalating conjugated oligoelectrolyte (COE), PTTP, was designed and synthesized with the goal of providing red‐shifted absorption spectra relative to previously synthesized COE analogs. Specifically, electron‐rich and electron‐poor subunits were introduced in the conjugated backbone to modulate the band gap. PTTP exhibits maxima of absorption at 507 nm and of emission at 725 nm. PTTP can also efficiently function to generate singlet oxygen in situ (ΦΔ≈20 %) and has appropriate topology and dimensions to interact with lipid membranes. The resulting rapid membrane insertion and sensitizing ability provide PTTP with a highly efficient antibacterial capability under a low light dose (0.6 J cm−2) toward Gram‐negative bacteria E. coli, making it a remarkably efficient optically mediated antimicrobial agent.
Photolysis of a High‐Spin Azidoiron(III) Complex Studied by Time‐Resolved Fourier‐Transform Infrared Spectroscopy
Authors: Torres‐Alacan Joel , Vöhringer Peter 
Published: March 2017

AbstractThe laser‐flash photolysis of the high‐spin azidoiron(III) complex [FeIII(Me3Cyclam‐ac)(N3)]PF6 ([1]PF6) in liquid acetonitrile solution at room temperature was explored by time‐resolved Fourier‐transform infrared spectroscopy. Excitation of [1] at 480 and 266 nm induced a photoreduction of the metal center and generated [FeII(Me3Cyclam‐ac)(NCCH3)]+ ([2]) and azidyl radicals. Both photoproducts were detected independently through scavenging experiments. The metal‐containing fragment was quenched with carbon monoxide to generate an iron(II) carbonyl complex, whereas the nitrogen‐containing fragment was quenched with iodide to form azide anions. In the presence of N3−, the photoreduction created the elusive hexanitrogen radical anion N6.− as a transient byproduct.