bluuebunny/crossref_metadata_2025_split huggingface dataset:
An eight-year follow-up study of Home Invasive Mechanical Ventilation in Finland
Authors: Kreivi Hanna-Riikka , Kotanen Petra , Siirala Waltteri 
Published: September 2024

Abstract
Background
Studies on long-term invasive mechanical ventilation (IMV) via tracheostomy in chronic respiratory insufficiency are limited. The aim of this study was to clarify the use of HIMV (home invasive mechanical ventilation) within the Finnish population and to analyze the characteristics and survival rate of HIMV patients from 2015 to 2022.

Methods
Data on HIMV patients was collected annually from all Finnish Hospital District patient registries between January 1, 2015, and December 31, 2022. Data included basic demographic data of the patients, underlying diagnosis, time from diagnosis to HIMV initiation, treatment duration, and mortality.

Results
This study included 179 patients. In 2015, there were 107 HIMV patients, and as of December 31, 2022, there were 95 patients. During the eight-year follow-up period, 84 patients (46.9%) died and there were 67 new patients between 2015 and2022. The prevalence of HIMV treatment in Finland was 2.4/100,000 on January 1,2015, and 1.8/ 100 000 on December 31, 2022. The average number of years living with HIMV for deceased patients at death was 10.1 ± 10.5 years largely depending on the underlying diagnosis. Of all the HIMV treatments, 32% were elective.

Conclusions
HIMV is a rare treatment in Finland, and based on our 8-year follow-up, prevalence of HIMV is diminishing. Given the high demands, and significant costs associated with HIMV, it is essential to prepare for long treatment, when planning HIMV. It is also advisable to prolong non-invasive ventilation (NIV) treatments for as long as possible.
A four year follow-up survey on the teledidactic TELUS ultrasound course: long-term benefits and implications
Authors: Höhne Elena , Schäfer Valentin S. , Neubauer Ricarda , Gotta Jennifer , Reschke Philipp , Wittek Agnes , Recker Florian 
Published: September 2024

Abstract
Background
The COVID-19 pandemic disrupted traditional medical education, prompting innovative teaching methods for practical skills training. The teledidactic TELUS ultrasound course, launched in 2020–2021, aimed to provide remote instruction in ultrasound techniques.

Objective
This study assesses the long-term impact of the teledidactic ultrasound course conducted during the study years on current clinical practice.

Methods
In 2024, a follow-up survey was conducted with former TELUS course students now practicing as physicians across various specialities. Participants rated their confidence in ultrasound examinations and its frequency in practice.

Results
21 out of 30 participants (70%) completed the survey. 71.4% rated the course experience as excellent (5/5 points). Most reported significant learning gains, especially in the FAST module. While all agreed the course enhanced their ultrasound skills, its impact on patient care received mixed reviews. Frequency of ultrasound use varied widely among specialities, with high use in surgery and internal medicine but less in psychiatry, neurology, and ophthalmology. Notably, 42,9% had not pursued further ultrasound training post-course.

Conclusion
The teledidactic ultrasound course effectively provided remote education, integrating skills into practice. Mixed reviews on patient care impact and speciality-specific ultrasound use suggest sustained integration depends on relevance and ongoing education. Self-assessment results support online ultrasound courses, indicating potential use in resource-limited or geographically constrained settings.
Real-world data to support post-market safety and performance of embolization coils: evidence generation from a medical device manufacturer and data institute partnership
Authors: Hochreiter-Hufford Amelia , Gatz Jennifer , Griggs Amy M. , Schoch Ryan D. , Birmingham Kimberly M. , Frederick Christopher , Price John , Snyder Scott 
Published: September 2024

Abstract
Background
Recognizing the limitations of pre-market clinical data, regulatory authorities have embraced total product lifecycle management with post-market surveillance (PMS) data to assess medical device safety and performance. One method of proactive PMS involves the analysis of real-world data (RWD) through retrospective review of electronic health records (EHR). Because EHRs are patient-centered and focused on providing tools that clinicians use to determine care rather than collecting information on individual medical products, the process of transforming RWD into real-world evidence (RWE) can be laborious, particularly for medical devices with broad clinical use and extended clinical follow-up. This study describes a method to extract RWD from EHR to generate RWE on the safety and performance of embolization coils.

Methods
Through a partnership between a non-profit data institute and a medical device manufacturer, information on implantable embolization coils’ use was extracted, linked, and analyzed from clinical data housed in an electronic data warehouse from the state of Indiana’s largest health system. To evaluate the performance and safety of the embolization coils, technical success and safety were defined as per the Society of Interventional Radiology guidelines. A multi-prong strategy including electronic and manual review of unstructured (clinical chart notes) and structured data (International Classification of Disease codes), was developed to identify patients with relevant devices and extract data related to the endpoints.

Results
A total of 323 patients were identified as treated using Cook Medical Tornado, Nester, or MReye embolization coils between 1 January 2014 and 31 December 2018. Available clinical follow-up for these patients was 1127 ± 719 days. Indications for use, adverse events, and procedural success rates were identified via automated extraction of structured data along with review of available unstructured data. The overall technical success rate was 96.7%, and the safety events rate was 5.3% with 18 major adverse events in 17 patients. The calculated technical success and safety rates met pre-established performance goals (≥ 85% for technical success and ≤ 12% for safety), highlighting the relevance of this surveillance method.

Conclusions
Generating RWE from RWD requires careful planning and execution. The process described herein provided valuable longitudinal data for PMS of real-world device safety and performance. This cost-effective approach can be translated to other medical devices and similar RWD database systems.
Clinicians’ perspectives of immersive tools in clinical mental health settings: a systematic scoping review
Authors: Cushnan Jessica , McCafferty Paul , Best Paul 
Published: September 2024

Abstract
Background
Virtual Reality in mental health treatment has potential to address a wide spectrum of psychological and neurocognitive disorders. Despite the proven benefits, integration into clinical practice faces significant challenges. There is a critical need for research into clinicians’ perceptions of virtual reality due to the gap between rapid technological advancements and their adoption in mental health services.

Method
A scoping review was conducted to comprehensively understand clinicians’ perspectives on the application of immersive virtual reality technologies within mental health settings. 4 data bases were searched, from inception, with the search areas of clinicians’, technology, perspectives and mental health. The scoping review followed the PRISMA-ScR checklist. All results were thematically analysed to identify and categorise themes with a focus on qualitative analyses of clinicians’ experiences and perceptions of VR applications in therapeutic contexts.

Results
17 articles were selected, encompassing a range of mental health settings. The findings indicate that the integration of VR in clinical environments is heavily influenced by clinicians’ knowledge and experience, with unfamiliarity often leading to scepticism. Positive attitudes towards VR, bolstered by direct experience and training, were found to drive acceptance, as clinicians’ acknowledged its potential to complement traditional therapies. However, there are still gaps in understanding VR’s therapeutic applications, particularly concerning its impact on human interaction and its suitability for specific patient groups. Balancing VR’s clinical benefits with ethical and safety concerns is crucial, especially when working with vulnerable populations. Furthermore, structural and administrative support is essential to overcoming the financial and logistical challenges of VR implementation, ensuring its safe and effective integration into mental health care.

Conclusion
While VR holds significant potential for enhancing mental health care, its successful integration into clinical practice necessitates addressing existing gaps in knowledge, training, and structural support. By carefully balancing its clinical benefits with ethical, practical, and safety considerations, VR can be effectively utilised as a valuable tool in mental health treatment, providing innovative solutions while ensuring that patient care remains paramount.
Mesothelin- and nucleolin-specific T cells from combined short peptides effectively kill triple-negative breast cancer cells
Authors: Thongchot Suyanee , Aksonnam Krittaya , Prasopsiri Jaturawitt , Warnnissorn Malee , Sa-nguanraksa Doonyapat , O-Charoenrat Pornchai , Thuwajit Peti , Yenchitsomanus Pa-thai , Thuwajit Chanitra 
Published: September 2024

Abstract
Background
Triple-negative breast cancer (TNBC), known for its aggressiveness and limited treatment options, presents a significant challenge. Adoptive cell transfer, involving the ex vivo generation of antigen-specific T cells from peripheral blood mononuclear cells (PBMCs), emerges as a promising approach. The overexpression of mesothelin (MSLN) and nucleolin (NCL) in TNBC samples underscores their potential as targets for T cell therapy. This study explored the efficacy of multi-peptide pulsing of PBMCs to generate MSLN/NCL-specific T cells targeting MSLN+/NCL+ TNBC cells.

Methods
TNBC patient samples were confirmed for both MSLN and NCL expression via immunohistochemistry. Synthesized MSLN and NCL peptides were combined and administered to activate PBMCs from healthy donors. The cancer-killing ability of the resultant T cells was assessed using crystal violet staining, and their subtypes and cytotoxic cytokines were characterized through flow cytometry and cytokine bead array.

Results
Findings showed that 85.3% (127/149) of TNBC cases were positive for either MSLN or NCL, or both; with single positivity rates for MSLN and NCL of 14.1% and 28.9%, respectively. MSLN and NCL peptides, with high binding affinity for HLA-A*02, were combined and introduced to activated PBMCs from healthy donors. The co-pulsed PBMCs significantly induced TEM and TEMRA CD3+/CD8+ T cells and IFN-γ production, compared to single-peptide pulsed or unpulsed conditions. Notably, MSLN/NCL-specific T cells successfully induced cell death in MSLN+/NCL+ MDA-MB-231 cells, releasing key cytotoxic factors such as perforin, granzymes A and B, Fas ligand, IFN-γ, and granulysin.

Conclusions
These findings serve as a proof-of-concept for using multiple immunogenic peptides as a novel therapeutic approach in TNBC patients.
Brugada syndrome precipitated by uncomplicated malaria treated with dihydroartemisinin piperaquine: a case report
Authors: Amir Muzakkir , Mukhtar Irmayanti , Tandean Pendrik , Rahmani Muhammad Zaki 
Published: September 2024

Abstract
Background
Cardiovascular events following anti-malarial treatment are reported infrequently; only a few studies have reported adverse outcomes. This case presentation emphasizes cardiological assessment of Brugada syndrome, presenting as life-threatening arrhythmia during anti-malarial treatment. Without screening and untreated, this disease may lead to sudden cardiac death.

Case presentation
This is a case of 23-year-old male who initially presented with palpitations followed by syncope and shortness of breath with a history of malaria. He had switched treatment from quinine to dihydroartemisinin-piperaquine (DHP). Further investigations revealed the ST elevation electrocardiogram pattern typical of Brugada syndrome, confirmed with flecainide challenge test. Subsequently, anti-malarial treatment was stopped and an Implantable Cardioverter Defibrillator (ICD) was inserted.

Conclusions
Another possible cause of arrhythmic events happened following anti-malarial consumption. This case highlights the possibility of proarrhytmogenic mechanism of malaria infection and anti-malarial drug resulting in typical manifestations of Brugada syndrome.
Aboriginal families living with MJD in remote Australia: questions of access and equity
Authors: Massey Libby , Gilroy John , Kowal Emma , Doolan Denise , Clough Alan 
Published: September 2024

AbstractManaging genetic disease using medically assisted reproductive technology is increasingly promoted as a feasible option, given revolutionary advances in genomics. Far less attention has been directed to the issue of whether there is equitable access to this option. Context and circumstance determine equitable access; however, reporting has drawn overwhelmingly from affluent Anglo-western populations in developed countries. The experiences of poorer, less educated subpopulations within affluent countries and populations in less developed countries are underreported. The ability of consumers to understand the opportunities and risks of medically assisted reproductive technology is likewise not well described in the literature despite significant technological complexity and evidence that genetic disease may be overrepresented within some disadvantaged population groups.Equity is achieved by identifying barriers and allocating appropriate resources to enable understanding and access. In the case of utilising medically assisted technology, social and power relationships, regulations, and the presumptions of authority figures and policymakers reduce equitable access. Physical or cultural marginalisation from mainstream health services may result in reduced access to genetic and prenatal testing, in-vitro fertilisation and genetic screening of embryos necessary for medically assisted reproduction. Cost and regulatory frameworks can likewise limit opportunities to engage with services. Moreover, the quality of the information provided to prospective users of the technology and how it is received governs understanding of prevention and inhibits adequately informed choice.Best practice care and adequately informed choice can only be achieved by conscientiously attending to these accessibility issues. Deep engagement with at-risk people and critical reflection on mainstream accepted standpoints is required. This paper outlines issues associated with engaging with medically assisted reproduction encountered by Aboriginal families living with Machado-Joseph Disease in some of the most remote areas of Australia. It is the right of these families to access such technologies regardless of where they live. Current barriers to access raise important questions for service providers with implications for practice as new technologies increasingly become part of standard medical care.
Recruitment of the m6A/m6Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48
Authors: Nabeel-Shah Syed , Pu Shuye , Burke Giovanni L. , Ahmed Nujhat , Braunschweig Ulrich , Farhangmehr Shaghayegh , Lee Hyunmin , Wu Mingkun , Ni Zuyao , Tang Hua , Zhong Guoqing , Marcon Edyta , Zhang Zhaolei , Blencowe Benjamin J. , Greenblatt Jack F. 
Published: September 2024

Abstract
Background
N6-methyladenosine (m6A), the most abundant internal modification on eukaryotic mRNA, and N6, 2′-O-dimethyladenosine (m6Am), are epitranscriptomic marks that function in multiple aspects of posttranscriptional regulation. Fat mass and obesity-associated protein (FTO) can remove both m6A and m6Am; however, little is known about how FTO achieves its substrate selectivity.

Results
Here, we demonstrate that ZBTB48, a C2H2-zinc finger protein that functions in telomere maintenance, associates with FTO and binds both mRNA and the telomere-associated regulatory RNA TERRA to regulate the functional interactions of FTO with target transcripts. Specifically, depletion of ZBTB48 affects targeting of FTO to sites of m6A/m6Am modification, changes cellular m6A/m6Am levels and, consequently, alters decay rates of target RNAs. ZBTB48 ablation also accelerates growth of HCT-116 colorectal cancer cells and modulates FTO-dependent regulation of Metastasis-associated protein 1 (MTA1) transcripts by controlling the binding to MTA1 mRNA of the m6A reader IGF2BP2.

Conclusions
Our findings thus uncover a previously unknown mechanism of posttranscriptional regulation in which ZBTB48 co-ordinates RNA-binding of the m6A/m6Am demethylase FTO to control expression of its target RNAs.
Astaxanthin attenuates glucose-induced liver injury in largemouth bass: role of p38MAPK and PI3K/Akt signaling pathways
Authors: Liao Zhihong , He Xuanshu , Chen Anqi , Zhong Jian , Lin Sihan , Guo Yucai , Cui Xin , Chen Baoyang , Zhao Wei , Niu Jin 
Published: September 2024

Abstract
Background
Astaxanthin (ASX) has been documented to exert beneficial influence on various processes in fish. Largemouth bass (Micropterus salmoides) serves as a common model for studying glucose-induced liver disease, making it imperative to investigate the regulatory mechanisms underlying its liver health.

Methods
Largemouth bass were fed with a control diet (CON), a high carbohydrate diet (HC), or a HC diet supplemented astaxanthin (HCA) for 8-weeks, followed by the glucose tolerance test (GTT). Primary hepatocytes were treated with low glucose and high glucose combined with different concentrations of astaxanthin for 48 h. The histopathology, enzymology, transcriptomics, molecular biology and cell biology were combined to investigate the mechanism of liver injury.

Results
This study provides evidence for the protective effects of ASX against growth performance reduction and hepatic liver injure in largemouth bass fed HC diet. In GTT, HCA diet exhibited an improvement in glucose tolerance following glucose loading. Although HCA diet did not restore the expression of insulin resistance-related genes in livers at different time during the GTT, the addition of ASX in the long-term HC diet did improve the insulin resistance pathway by regulating the PTP1B/PI3K/Akt signaling pathway. Hepatic transcriptome analyses showed that ASX plays an essential role in the modulation of glucose homeostasis in response to treated with HC diet. In in vitro study, ASX treatment resulted in an exaltation in cell viability and a reduction in the rate of cell apoptosis and reactive oxygen species (ROS). Additionally, astaxanthin was observed to improve apoptosis induced by high-glucose via p38MAPK/bcl-2/caspase-3 signaling pathway.

Conclusions
Astaxanthin exhibited a protective effect against apoptosis by regulating p38MAPK/bcl-2/caspase-3 pathway, and ameliorated insulin resistance by activating the PTP1B/PI3K/Akt pathway. This study elucidated the mechanism of astaxanthin in the liver injury of largemouth bass from a new perspective and provided a new target for the treatment of insulin resistance.

Graphical Abstract
Effects of warming on soil fungal community and its function in a temperate steppe
Authors: Yu Yang , Chen Xin , Yi Yin , Xiao Chunwang 
Published: September 2024

Abstract
Background
The potential effects of global warming on soil fungal communities and their functions remain uncertain. To address this issue, we investigated the effects of 3-year simulated field warming on the community and function of fungi in a temperate steppe of Inner Mongolia, northern China.

Methods
The diversity and structure of the fungal community were measured by high-throughput sequencing. The functionality of fungal communities was identified by comparison with the ITS reference database.

Results
Our results showed that warming did not affect the diversity of fungi, but significantly increased the complexity of the fungal community, with fungal taxa more closely associating with each other. We observed that plant pathogens and arbuscular mycorrhizal fungi were the most abundant functional groups. Meanwhile, warming significantly decreased the relative abundance of animal pathogens.

Conclusions
Warming significantly increased the complexity of the fungal community, with soil pH being the main factor affecting soil fungal function. Our findings emphasize that the response of the fungal community and its functional groups to warming has significant implications for ecosystem biogeochemical cycling.
Midwestern specialty crop impacts on the environment and health: a scoping review
Authors: Balis Laura E. , Shaw Emily , Fung Uy Whitney , Nelson Katie , Isack Maryan , Flournoy Laura , Vest Daniele , Deelo Jessie , Yaroch Amy L. 
Published: September 2024

Abstract
Introduction
The United States food system mainly relies on monoculture farming, leading to negative impacts on human and environmental health. Transitioning to specialty crop production (fruits, vegetables, and tree nuts) could alleviate challenges. The goal of this scoping review was to understand environmental and health impacts of locally distributed specialty crops in the Midwest.

Methods
Researchers searched databases for peer-reviewed literature and agricultural websites for grey literature. Inclusion criteria were specialty crop production; environmental, economic, or health outcomes; Midwest location; and local distribution. Researchers charted data based on the reach, effectiveness, adoption, implementation, and maintenance framework.

Results
Grey (n = 9) and peer-reviewed (n = 19) sources met inclusion criteria. Sources reported specialty crops reached diverse populations through community gardens and farmers’ markets with positive impacts on nutritional intake. Effectiveness of production practices on soil and plant quality and greenhouse gas emissions was mixed.

Conclusions
Local specialty crop production shows promise, but more rigorous study designs with long-term follow-up are needed.
Transitioning to sustainable food systems in a changing climate and gender equality: a brief review
Authors: Naheed Sanober , Rukhsana  
Published: September 2024

AbstractFood security is unquestionably crucial to society's well-being. The world is experiencing unprecedented global shocks; therefore, it is critical to protect the agri-food systems. This concept is broad and complex, with various interpretations around the globe. The problems of social equity, environmental sustainability, and food security are interconnected and are rarely best addressed separately. The failure to address the farming sector, particularly that of women farmers, is the basis of the current discussion. Creating sustainable food systems requires moving from an agriculture-centred to a food system policy and research framework. This will be fundamental to fostering the complex and holistic transformation necessary to achieve sustainable food systems, which is, in turn, a prerequisite to achieving sustainable food and nutrition security. Therefore, the current study aims to establish the link between women's empowerment and food security. The study used a systematic literature review approach based on secondary data available in relevant and contemporary literature. The findings showed the scarcity of research in areas of gender response to climate change and coping strategies and cultural biases against women.Moreover, most policies designed to improve and expand local communities’ capacity for adaptation are gender-neutral. Interdisciplinary research and innovation are likely to be the most effective methods for tackling food insecurity while considering its complex social, economic, and environmental components. The objective is to advance sustainable solutions. Therefore, in addition to academic thought, a focused approach to the issue is required; one must act following reality.