bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell‐free production and streamlined assay of cytosol‐penetrating antibodies
Authors: Min Seung Eui , Lee Kyung‐Ho , Park Seong‐Wook , Yoo Tae Hyeon , Oh Chan Hee , Park Ji‐Ho , Yang Sung Yun , Kim Yong‐Sung , Kim Dong‐Myung 
Published: April 2016

ABSTRACTAntibodies that target intracellular proteins hold great promise in the development of novel therapeutic interventions for various diseases. In particular, antibodies that can cross cellular membranes have potential applications in controlling disease‐related intracellular protein–protein interactions. Given the large number of cytosolic proteins and complicated interactions that are potentially involved in disease development, discovery of antibodies targeting intracellular proteins requires iterative cycles of expression and assessment of candidate antibodies. Because current cell‐based expression methods do not provide sufficient throughput for production and assay of cytosol‐penetrating antibodies, we integrated a cell‐free protein synthesis system designed to provide optimal conditions for production of functional antibodies with a cytosol‐penetration assay. The proposed approach of consolidating cell‐free synthesis and cell‐based assay will substantially expand the capability of discovering and engineering antibodies that can cross the cell membrane and effectively control protein‐mediated cellular functions. Biotechnol. Bioeng. 2016;113: 2107–2112. © 2016 Wiley Periodicals, Inc.
Multicentre study of neoadjuvant chemotherapy for stage I and II oesophageal cancer
Authors: Bekkar S , Gronnier C , Renaud F , Duhamel A , Pasquer A , Théreaux J , Gagnière J , Meunier B , Collet D , Mariette C , Dhahri A , Lignier D , Cossé C , Regimbeau J-M , Luc G , Cabau M , Jougon J , Badic B , Lozach P , Bail J P , Cappeliez S , El Nakadi I , Lebreton G , Alves A , Flamein R , Pezet D , Pipitone F , Stan-Iuga B , Contival N , Pappalardo E , Coueffe X , Msika S , Mantziari S , Demartines N , Hec F , Vanderbeken M , Tessier W , Briez N , Fredon F , Gainant A , Mathonnet M , Bigourdan J M , Mezoughi S , Ducerf C , Baulieux J , Mabrut J-Y , Bigourdan J M , Baraket O , Poncet G , Adam M , Vaudoyer D , Jourdan Enfer P , Villeneuve L , Glehen O , Coste T , Fabre J-M , Marchal F , Frisoni R , Ayav A , Brunaud L , Bresler L , Cohen C , Aze O , Venissac N , Pop D , Mouroux J , Donici I , Prudhomme M , Felli E , Lisunfui S , Seman M , Godiris Petit G , Karoui M , Tresallet C , Ménégaux F , Vaillant J-C , Hannoun L , Malgras B , Lantuas D , Pautrat K , Pocard M , Valleur P , Lefevre J H , Chafai N , Balladur P , Lefrançois M , Parc Y , Paye F , Tiret E , Nedelcu M , Laface L , Perniceni T , Gayet B , Turner K , Filipello A , Porcheron J , Tiffet O , Kamlet N , Chemaly R , Klipfel A , Pessaux P , Brigand C , Rohr S , Carrère N , Da Re C , Dumont F , Goéré D , Elias D , Bertrand C 
Published: April 2016

Abstract

Background
The benefit of neoadjuvant chemotherapy (NCT) for early-stage oesophageal cancer is unknown. The aim of this study was to assess whether NCT improves the outcome of patients with stage I or II disease.

Methods
Data were collected from 30 European centres from 2000 to 2010. Patients who received NCT for stage I or II oesophageal cancer were compared with patients who underwent primary surgery with regard to postoperative morbidity, mortality, and overall and disease-free survival. Propensity score matching was used to adjust for differences in baseline characteristics.

Results
Of 1173 patients recruited (181 NCT, 992 primary surgery), 651 (55·5 per cent) had clinical stage I disease and 522 (44·5 per cent) had stage II disease. Comparisons of the NCT and primary surgery groups in the matched population (181 patients in each group) revealed in-hospital mortality rates of 4·4 and 5·5 per cent respectively (P = 0·660), R0 resection rates of 91·7 and 86·7 per cent (P = 0·338), 5-year overall survival rates of 47·7 and 38·6 per cent (hazard ratio (HR) 0·68, 95 per cent c.i. 0·49 to 0·93; P = 0·016), and 5-year disease-free survival rates of 44·9 and 36·1 per cent (HR 0·68, 0·50 to 0·93; P = 0·017).

Conclusion
NCT was associated with better overall and disease-free survival in patients with stage I or II oesophageal cancer, without increasing postoperative morbidity.
1‐Acetyl‐3‐[(3R)‐hydroxyfatty acyl]glycerols: Lipid Compounds from Euphrasia rostkoviana Hayne and E. tetraquetra (Bréb.) Arrond.
Authors: Lorenz Peter , Knittel Diana N. , Conrad Jürgen , Lotter Eva M. , Heilmann Jörg , Stintzing Florian C. , Kammerer Dietmar R. 
Published: April 2016

Five homologous acetylated acylglycerols of 3‐hydroxyfatty acids (chain lengths C(14) – C(18)), named euphrasianins A – E, were characterized for the first time in Euphrasia rostkoviana Hayne (Orobanchaceae) by gas chromatography/mass spectrometry (GC/MS) and high‐performance liquid chromatography/atmospheric pressure chemical ionization‐mass spectrometry (HPLC/APCI‐MSn). In addition to mass spectrometric data, structures of euphrasianins were verified via a three‐step total synthesis of one representative homologue (euphrasianin A). The structure of the latter was confirmed by 1D‐ and 2D‐NMR experiments as well as high‐resolution electrospray ionization‐mass spectrometry (HR‐ESI‐MS). The absolute configuration of the 3‐hydroxyfatty acid moiety at C(3) was found to be R in the natural euphrasianins, which was determined by alkaline hydrolysis and methylation of a purified fraction, followed by chiral GC analysis. Furthermore, in extracts of Euphrasia tetraquetra (Bréb.) Arrond. euphrasianins C and E were detected exclusively, indicating that this subclass of lipid constituents is possibly valuable for fingerprinting methods.
Three‐Dimensional Chemical Structure Search Using the Conformational Code for Organic Molecules (CCOM) Program
Authors: Izumi Hiroshi , Nafie Laurence A. , Dukor Rina K. 
Published: April 2016

AbstractSearching the 3D structural fragments of organic molecules is challenging because of structural differences between X‐ray and theoretically calculated geometries and the conformational flexibility of substituents. The codification program called Conformational Code for Organic Molecules (CCOM) can be used to unambiguously convert 3D conformational data for various molecules to 1D data. Two deviations from Rule E‐5.6 of the International Union of Pure and Applied Chemistry (IUPAC) Rules for Nomenclature of Organic Chemistry were introduced to the CCOM program for 3D fragment searching. First, the search for the highest priority atom was limited to a distance of two bonds from the center bond for dihedral angle determination. Second, for indistinguishable atoms in experimentally observed solution structures, the smallest number of atom index in the molecular model was chosen as the priority atom for dihedral angle determination. A search of the 3D conformational fragment mb_3a6c4c of mevastatin (1) in combination with the SMiles ARbitrary Target Specification (SMARTS) description suggested that a change in the conformation of this fragment may be the driving force for dissociation of mevastatin from its target protein. Chirality 28:370–375, 2016. © 2016 Wiley Periodicals, Inc.
Hydrogenation of citral over nitrogen‐doped carbon nanofibre‐supported nickel catalyst
Authors: Gryglewicz Stanisław , Śliwak Agata , Ćwikła Joanna , Gryglewicz Grażyna 
Published: April 2016

AbstractHerringbone carbon nanofibres (hCNFs) and nitrogen‐doped carbon nanofibres with 0.115 g/g (11.5 %) of N (N‐hCNFs) were used as the supports of nickel catalysts in the liquid phase hydrogenation of citral using toluene as a solvent. Over 96 % selectivity to citronellal was obtained at 150 °C for both catalysts. At the reaction temperature of 200 °C, reasonable amounts of isopulegol were formed additionally as a result of citronellal cyclization. The catalytic activity of Ni nanoparticles was significantly improved when supported on CNFs doped with nitrogen. The hydrogenation of cis‐citral was remarkably favoured over the trans‐citral isomer regardless of the reaction temperature. The hydrogenation of citronellal under the same process conditions was also studied. The sequential‐parallel reactions network for the hydrogenation of citral under the applied conditions was proposed and the kinetic constants were evaluated, assuming pseudo‐first order kinetics of the hydrogenation process.
Radiation Efficiency of a Multipole Acoustic Source in a Fluid‐Filled Borehole
Authors: Jing‐Ji CAO , Xiao‐Ming TANG , Yuan‐Da SU , Zhou‐Tuo WEI 
Published: April 2016

AbstractThe single‐well imaging using reflected wave field has become an important well logging technology to detect formation geologic structures outside a borehole. For this application, various types of sources are used, including monopole, dipole and quadrupole sources. Using the concept of acoustic energy flow density, we propose a method to evaluate the radiation property and efficiency of a borehole source by comparing the energy flow of guided waves along borehole to that of radiated waves away from borehole. Using this method, we study the frequency variation characteristics of the energy flow and radiation efficiency for a multipole source in a fluid‐filled borehole. Our results demonstrate that the radiation efficiency and dominant frequency of monopole, dipole and quadrupole sources are all different. The radiation efficiency of monopole is very low in the frequency range below 5 kHz, because the Stoneley wave carries almost all the energy away along borehole. In contrast, the dipole radiation efficiency is much greater than the monopole source in the low‐frequency range. This result explains why the dipole source is a better low‐frequency source than the monopole.
Evaluation of the visibility of colored objects under led lighting with various correlated color temperatures
Authors: Hong Seongkwan , Kim Intae , Kim Hyunsun , Sohn Arom , Choi An‐Seop , Sung Minki , Jeong Jae‐Weon 
Published: April 2016

AbstractThis study proposes a “visibility” concept of colored objects that includes a combination of color brightness and appearance perception of colored objects that are illuminated by seven LED lights (each with a CIE color rendering index under 80 but with various correlated color temperatures). To determine the brightness perception of colored objects, luminance calculations and measurements were conducted. The areas of the “color brightness graph” in this study correspond to the results of eight different color sample papers under each of the LED lights with different CCTs. The luminance values were calculated by multiplying the SPD by the spectral luminous efficiency and spectral reflectance of a colored object. The luminance values were measured under these conditions to identify the relationship between the luminance calculations and the measured values. Efficient CCTs were identified for each color sample in terms of the brightness of the colored object. Further subjective evaluations were also conducted to identify the relationship between luminance values and subjective brightness perception. In addition, subjective evaluations of the color appearance perception were conducted to identify the overall visibility concept of colored objects. The subjective evaluations included brightness perception, color appearance, and similarity of the reference light source. The visibility of the colored objects was analyzed according to the results of the brightness and appearance perception of the colored objects that were illuminated by LED lights with various CCTs. © 2016 Wiley Periodicals, Inc. Col Res Appl, 42, 78–88, 2017
Self‐Assembly of the Cyclic Lipopeptide Daptomycin: Spherical Micelle Formation Does Not Depend on the Presence of Calcium Chloride
Authors: Kirkham Steven , Castelletto Valeria , Hamley Ian William , Inoue Katsuaki , Rambo Robert , Reza Mehedi , Ruokolainen Janne 
Published: April 2016

AbstractThe cyclic lipopeptide Daptomycin, used as a treatment for infections where antimicrobial resistance is observed, is shown to self‐assemble into spherical micelles above a critical aggregation concentration. Micelles are observed either in the absence or presence of CaCl2, in contrast to claims in the literature that CaCl2 is required for micellization.
Quality and exploitation of umbilical cord blood for cell therapy: Are we beyond our capabilities?
Authors: Roura Santiago , Pujal Josep Maria , Gálvez‐Montón Carolina , Bayes‐Genis Antoni 
Published: April 2016

AbstractThere is increasing interest in identifying novel stem cell sources for application in emerging cell therapies. In this context, umbilical cord blood (UCB) shows great promise in multiple clinical settings. The number of UCB banks has therefore increased worldwide, with the objective of preserving potentially life‐saving cells that are usually discarded after birth. After a rather long and costly processing procedure, the resultant UCB‐derived cell products are cryopreserved until transplantation to patients. However, in many cases, only a small proportion of administered cells engraft successfully. Thus, can we do any better regarding current UCB‐based therapeutic approaches? Here we discuss concerns about the use of UCB that are not critically pondered by researchers, clinicians, and banking services, including wasting samples with small volumes and the need for more reliable quality and functional controls to ensure the biological activity of stem cells and subsequent engraftment and treatment efficacy. Finally, we appeal for collaborative agreements between research institutions and UCB banks in order to redirect currently discarded small‐volume UCB units for basic and clinical research purposes. Developmental Dynamics 245:710–717, 2016. © 2016 Wiley Periodicals, Inc.
No effect of blood sampling or phytohaemagglutinin injection on postfledging survival in a wild songbird
Authors: Bowers Emerson Keith , Sakaluk Scott K. , Thompson Charles F. 
Published: April 2016

AbstractThe injection of phytohaemagglutinin (PHA) and sampling of blood are widely used in studies of wild vertebrates to assess components of immune and endocrine function and health state and to obtain genetic material. Despite the pervasive use of these techniques in the life sciences, their potential effects on survival are rarely considered. For example, whether injection of the immunogen PHA into body parts critical for locomotion (e.g., the prepatagium, or wing web, in birds) affects survival has not been tested. Here, we test whether injection of PHA into the wing web and blood sampling from nestling house wrens affects their subsequent recruitment and survival as breeding adults. Capture‐mark‐recapture analysis on a large sample of young (N = 20,152 fledglings from 3959 broods) treated over 10 years revealed that neither PHA injection nor blood sampling affected individual survival and detection probability. Recruitment as a breeder varied among years, but this variation was not attributable to sampling effort, or the percent of all adults identified at the nest during a given year. Variation in the percent of adults identified was primarily attributable to the effect of nest depredation on our ability to capture nesting pairs. Our results indicating lack of an effect of blood sampling and immune stimulation on survival are encouraging, but we recommend further work to assess the potential negative effects of all commonly used techniques on the survival of study subjects in the wild, including the potential costs associated with mounting various immunological responses.
Population‐specific effects of developmental temperature on body condition and jumping performance of a widespread European frog
Authors: Drakulić Sanja , Feldhaar Heike , Lisičić Duje , Mioč Mia , Cizelj Ivan , Seiler Michael , Spatz Theresa , Rödel Mark‐Oliver 
Published: April 2016

AbstractAll physiological processes of ectotherms depend on environmental temperature. Thus, adaptation of physiological mechanisms to the thermal environments is important for achieving optimal performance and fitness. The European Common Frog, Rana temporaria, is widely distributed across different thermal habitats. This makes it an exceptional model for studying the adaptations to different thermal conditions. We raised tadpoles from Germany and Croatia at two constant temperature treatments (15°C, 20°C), and under natural temperature fluctuations (in outdoor treatments), and tested how different developmental temperatures affected developmental traits, that is, length of larval development, morphometrics, and body condition, as well as jumping performance of metamorphs. Our results revealed population‐specific differences in developmental time, body condition, and jumping performance. Croatian frogs developed faster in all treatments, were heavier, in better body condition, and had longer hind limbs and better jumping abilities than German metamorphs. The populations further differed in thermal sensitivity of jumping performance. While metamorphs from Croatia increased their jumping performance with higher temperatures, German metamorphs reached their performance maximum at lower temperatures. These population‐specific differences in common environments indicate local genetic adaptation, with southern populations being better adapted to higher temperatures than those from north of the Alps.
Carbon storage in China's forest ecosystems: estimation by different integrative methods
Authors: Peng Shunlei , Wen Ding , He Nianpeng , Yu Guirui , Ma Anna , Wang Qiufeng 
Published: April 2016

AbstractCarbon (C) storage for all the components, especially dead mass and soil organic carbon, was rarely reported and remained uncertainty in China's forest ecosystems. This study used field‐measured data published between 2004 and 2014 to estimate C storage by three forest type classifications and three spatial interpolations and assessed the uncertainty in C storage resulting from different integrative methods in China's forest ecosystems. The results showed that C storage in China's forest ecosystems ranged from 30.99 to 34.96 Pg C by the six integrative methods. We detected 5.0% variation (coefficient of variation, CV, %) among the six methods, which was influenced mainly by soil C estimates. Soil C density and storage in the 0–100 cm soil layer were estimated to be 136.11–153.16 Mg C·ha−1 and 20.63–23.21 Pg C, respectively. Dead mass C density and storage were estimated to be 3.66–5.41 Mg C·ha−1 and 0.68–0.82 Pg C, respectively. Mean C storage in China's forest ecosystems estimated by the six integrative methods was 8.557 Pg C (25.8%) for aboveground biomass, 1.950 Pg C (5.9%) for belowground biomass, 0.697 Pg C (2.1%) for dead mass, and 21.958 Pg C (66.2%) for soil organic C in the 0–100 cm soil layer. The R:S ratio was 0.23, and C storage in the soil was 2.1 times greater than in the vegetation. Carbon storage estimates with respect to forest type classification (38 forest subtypes) were closer to the average value than those calculated using the spatial interpolation methods. Variance among different methods and data sources may partially explain the high uncertainty of C storage detected by different studies. This study demonstrates the importance of using multimethodological approaches to estimate C storage accurately in the large‐scale forest ecosystems.
Face‐Shared Octahedral Dimer In2O7S2 in the Non‐Centrosymmetric Barium Indiumsilicate Oxysulfide Ba2In2Si3O10S
Authors: Guo Wei‐Huan , Jiang Xiao‐Ming , Liu Bin‐Wen , Wu Jia‐Wei , Li Shu‐Fang , Zeng Hui‐Yi , Guo Guo‐Cong , Huang Jin‐Shun 
Published: April 2016

AbstractThe first indiumsilicate oxysulfide Ba2In2Si3O10S (1) was obtained by an alkali polychalcogenide flux method through a high‐temperature solid‐state reaction. It crystallizes in the non‐centrosymmetric space group Pca21 (No. 29), with a = 23.060(6), b = 5.2954(6), c = 8.779(2) Å, and Z = 4. Its three‐dimensional (3D) framework is constructed from one‐dimensional (1D) infinite ∞1[In2O7S2/2] double octahedral chains bridged by corner‐sharing Si tetrahedral trimers [Si3O10]8–, yielding a 3D framework with the channels filled with Ba2+ cations along the b direction. It is unprecedented in that the two distorted indium octahedra in 1 face‐share to form a [In2O7S2] dimer in indium oxides and oxide chalcogenides.
Next‐Generation Summative English Language Proficiency Assessments for English Learners: Priorities for Policy and Research
Authors: Wolf Mikyung Kim , Guzman‐Orth Danielle , Hauck Maurice Cogan 
Published: April 2016

AbstractThis paper is the third in a series concerning English language proficiency (ELP) assessments for K–12 English learners (ELs). The series, produced from Educational Testing Service (ETS), is intended to provide theory‐ and evidence‐based principles and recommendations for improving next‐generation ELP assessment systems, policies, and practices and to stimulate discussion on better serving K–12 EL students. The first paper articulated a high‐level conceptualization of next‐generation ELP assessment systems (Hauck, Wolf, & Mislevy, 2016). The second paper addressed accessibility issues in the context of ELP assessments for ELs and ELs with disabilities (Guzman‐Orth, Laitusis, Thurlow, & Christensen, 2016). The fourth paper dealt with one of the major uses of ELP assessments—the initial identification and classification of ELs (Lopez, Pooler, & Linquanti, 2016). The present paper focuses on summative ELP assessments that states use for accountability purposes. Based on the conversation that took place at the K–12 ELP Assessment Working Meeting on summative ELP assessments, we identify critical policy and research issues. We also highlight the challenges associated with measuring ELP in a diverse group of EL students and recommend ways to embrace and support EL students through shared goals and active communication across federal agencies, state agencies, local agencies, researchers, and practitioners.
Creating a Next‐Generation System of K–12 English Learner Language Proficiency Assessments
Authors: Hauck Maurice Cogan , Wolf Mikyung Kim , Mislevy Robert 
Published: April 2016

AbstractThis paper is the first in a series from Educational Testing Service (ETS) concerning English language proficiency (ELP) assessments for K–12 English learners (ELs). The goal of this paper, and the series, is to present research‐based ideas, principles, and recommendations for consideration by those who are conceptualizing, developing, and implementing ELP assessments for K–12 ELs and by all stakeholders in their education and assessment. We also hope to contribute to the active current discussion in the field on improving the instruction and assessment of EL students as well as contribute to bringing these ideas into practice. This paper articulates a high‐level vision for a next‐generation assessment system serving K–12 English learners with a brief overview of the current state of the art for K–12 ELP assessments and the context of current reforms. In addition, the paper discusses the role of digital technology in next‐generation K‐12 ELP assessment systems, noting the distinct conceptual and practical advantages that digital technology offers in assessing ELP as well as principles for evaluating the potential challenges of implementing a digitally based assessment system against these benefits. A proposed theory of action for K–12 ELP assessment systems is also discussed in this paper. The second paper in the series addressed accessibility issues in the context of ELP assessments for ELs and ELs with disabilities (Guzman‐Orth, Laitusis, Thurlow, & Christensen, 2016), the third paper addressed issues related to summative ELP assessments that emerged from the presentations and discussions at the English Language Proficiency Assessment Research working meeting (Wolf, Guzman‐Orth, & Hauck, 2016), and the fourth paper focused on a key concern within such systems—the initial identification and classification of ELs (Lopez, Pooler, & Linquanti, 2016).