bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of PHD3 by salidroside promotes neovascularization through cell–cell communications mediated by muscle-secreted angiogenic factors
Authors: Zhang Jing , Kasim Vivi , Xie Yu-Dan , Huang Can , Sisjayawan Julita , Dwi Ariyanti Agnes , Yan Xue-Song , Wu Xiao-Yan , Liu Cai-Ping , Yang Li , Miyagishi Makoto , Wu Shou-Rong 
Published: March 2017

AbstractTherapeutic angiogenesis has been considered as a potential strategy for treating peripheral artery diseases including hind-limb ischemia (HLI); however, no effective drug-based treatment is currently available. Here we showed that intramuscular administration of salidroside, an active compound of Chinese herb Rhodiola, could robustly enhance blood perfusion recovery by promoting neovascularization in HLI mice. We revealed that salidroside promoted skeletal muscle cell migration and paracrine function through inhibiting the transcriptional level of prolyl-hydroxylase domain 3 (PHD3) without affecting PHD1 and PHD2. Paracrine signals from salidroside-treated skeletal muscle cells enhanced endothelial and smooth muscle cells migration, while inhibition of FGF2/FGF2R and PDGF-BB/PDGFR-β pathways abolished this effect, as well as neovascularization in HLI mice. Furthermore, we elucidated that salidroside inhibition on PHD3 might occur through estrogen receptor alpha (ERα). Together, our findings highlights the potential application of salidroside as a novel pharmalogical inhibitor of ERα/PHD3 axis for therapeutic angiogenesis in HLI diseases.
Diverse origin of mitochondrial lineages in Iron Age Black Sea Scythians
Authors: Juras Anna , Krzewińska Maja , Nikitin Alexey G. , Ehler Edvard , Chyleński Maciej , Łukasik Sylwia , Krenz-Niedbała Marta , Sinika Vitaly , Piontek Janusz , Ivanova Svetlana , Dabert Miroslawa , Götherström Anders 
Published: March 2017

AbstractScythians were nomadic and semi-nomadic people that ruled the Eurasian steppe during much of the first millennium BCE. While having been extensively studied by archaeology, very little is known about their genetic identity. To fill this gap, we analyzed ancient mitochondrial DNA (mtDNA) from Scythians of the North Pontic Region (NPR) and successfully retrieved 19 whole mtDNA genomes. We have identified three potential mtDNA lineage ancestries of the NPR Scythians tracing back to hunter-gatherer and nomadic populations of east and west Eurasia as well as the Neolithic farming expansion into Europe. One third of all mt lineages in our dataset belonged to subdivisions of mt haplogroup U5. A comparison of NPR Scythian mtDNA linages with other contemporaneous Scythian groups, the Saka and the Pazyryks, reveals a common mtDNA package comprised of haplogroups H/H5, U5a, A, D/D4, and F1/F2. Of these, west Eurasian lineages show a downward cline in the west-east direction while east Eurasian haplogroups display the opposite trajectory. An overall similarity in mtDNA lineages of the NPR Scythians was found with the late Bronze Age Srubnaya population of the Northern Black Sea region which supports the archaeological hypothesis suggesting Srubnaya people as ancestors of the NPR Scythians.
An optimized microfabricated platform for the optical generation and detection of hyperpolarized 129Xe
Authors: Kennedy Daniel J. , Seltzer Scott J. , Jiménez-Martínez Ricardo , Ring Hattie L. , Malecek Nicolas S. , Knappe Svenja , Donley Elizabeth A. , Kitching John , Bajaj Vikram S. , Pines Alexander 
Published: March 2017

AbstractLow thermal-equilibrium nuclear spin polarizations and the need for sophisticated instrumentation render conventional nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) incompatible with small-scale microfluidic devices. Hyperpolarized 129Xe gas has found use in the study of many materials but has required very large and expensive instrumentation. Recently a microfabricated device with modest instrumentation demonstrated all-optical hyperpolarization and detection of 129Xe gas. This device was limited by 129Xe polarizations less than 1%, 129Xe NMR signals smaller than 20 nT, and transport of hyperpolarized 129Xe over millimeter lengths. Higher polarizations, versatile detection schemes, and flow of 129Xe over larger distances are desirable for wider applications. Here we demonstrate an ultra-sensitive microfabricated platform that achieves 129Xe polarizations reaching 7%, NMR signals exceeding 1 μT, lifetimes up to 6 s, and simultaneous two-mode detection, consisting of a high-sensitivity in situ channel with signal-to-noise of 105 and a lower-sensitivity ex situ detection channel which may be useful in a wider variety of conditions. 129Xe is hyperpolarized and detected in locations more than 1 cm apart. Our versatile device is an optimal platform for microfluidic magnetic resonance in particular, but equally attractive for wider nuclear spin applications benefitting from ultra-sensitive detection, long coherences, and simple instrumentation.
Effect of ionizing radiation on the proliferation of human embryonic stem cells
Authors: Panyutin Irina V. , Holar Sonia A. , Neumann Ronald D. , Panyutin Igor G. 
Published: March 2017

AbstractWe studied the effect of ionizing radiation (IR) on continuous growth of seven hESC lines. Cells were exposed to 0, 0.2, or 1 Gy of X-rays, and the growth rates of cell populations were assessed by measuring areas of the same individual colonies versus time. The population doubling times (DT) of sham-irradiated cells varied from 18.9 to 28.7 hours for different cell lines. All cell lines showed similar reaction to IR, i.e. cell populations dropped within 24–48 hours post IR; after that they recovered and grew with the same rate as the sham-irradiated cells. The relative cell survival (RCS), i.e. the ratio of normalized cell population in the irradiated samples to that of the sham-irradiated ones varied from 0.6 to 0.8 after 0.2 Gy, and from 0.1 to 0.2 after 1 Gy IR for different cell lines. We found that the RCS values of hESC lines correlated directly with their DT, i.e. the faster cells grow the more radiosensitive they are. We also found that DT and RCS values of individual colonies varied significantly within all hESC lines. We believe that the method developed herein can be useful for assessing other cytotoxic insults on cultures of hESC.
Superconducting gap structure of FeSe
Authors: Jiao Lin , Huang Chien-Lung , Rößler Sahana , Koz Cevriye , Rößler Ulrich K. , Schwarz Ulrich , Wirth Steffen 
Published: March 2017

AbstractThe microscopic mechanism governing the zero-resistance flow of current in some iron-based, high-temperature superconducting materials is not well understood up to now. A central issue concerning the investigation of these materials is their superconducting gap symmetry and structure. Here we present a combined study of low-temperature specific heat and scanning tunnelling microscopy measurements on single crystalline FeSe. The results reveal the existence of at least two superconducting gaps which can be represented by a phenomenological two-band model. The analysis of the specific heat suggests significant anisotropy in the gap magnitude with deep gap minima. The tunneling spectra display an overall “U”-shaped gap close to the Fermi level away as well as on top of twin boundaries. These results are compatible with the anisotropic nodeless models describing superconductivity in FeSe.
High glucose alters tendon homeostasis through downregulation of the AMPK/Egr1 pathway
Authors: Wu Yu-Fu , Wang Hsing-Kuo , Chang Hong-Wei , Sun Jingyu , Sun Jui-Sheng , Chao Yuan-Hung 
Published: March 2017

AbstractDiabetes mellitus (DM) is associated with higher risk of tendinopathy, which reduces tolerance to exercise and functional activities and affects lifestyle and glycemic control. Expression of tendon-related genes and matrix metabolism in tenocytes are essential for maintaining physiological functions of tendon. However, the molecular mechanisms involved in diabetic tendinopathy remain unclear. We hypothesized that high glucose (HG) alters the characteristics of tenocyte. Using in vitro 2-week culture of tenocytes, we found that expression of tendon-related genes, including Egr1, Mkx, TGF-β1, Col1a2, and Bgn, was significantly decreased in HG culture and that higher glucose consumption occurred. Down-regulation of Egr1 by siRNA decreased Scx, Mkx, TGF-β1, Col1a1, Col1a2, and Bgn expression. Blocking AMPK activation with Compound C reduced the expression of Egr1, Scx, TGF-β1, Col1a1, Col1a2, and Bgn in the low glucose condition. In addition, histological examination of tendons from diabetic mice displayed larger interfibrillar space and uneven glycoprotein deposition. Thus, we concluded that high glucose alters tendon homeostasis through downregulation of the AMPK/Egr1 pathway and the expression of downstream tendon-related genes in tenocytes. The findings render a molecular basis of the mechanism of diabetic tendinopathy and may help develop preventive and therapeutic strategies for the pathology.
Neoagaro-oligosaccharide monomers inhibit inflammation in LPS-stimulated macrophages through suppression of MAPK and NF-κB pathways
Authors: Wang Wei , Liu Pei , Hao Cui , Wu Lijuan , Wan Wenjin , Mao Xiangzhao 
Published: March 2017

AbstractNeoagaro-oligosaccharides derived from agarose have been demonstrated to possess a variety of biological activities, such as anti-bacteria and anti-oxidative activities. In this study, we mainly explored the inhibitory effects and the mechanisms of neoagaro-oligosaccharide monomers against LPS-induced inflammatory responses in mouse macrophage RAW264.7 cells. The results indicated that neoagaro-oligosaccharide monomers especially neoagarotetraose could significantly reduce the production and release of NO in LPS-induced macrophages. Neoagarotetraose significantly suppressed the expression and secretion of inducible nitric oxide synthase (iNOS) and proinflammatory cytokines such as TNF-α and IL-6. The inhibition mechanisms may be associated with the inhibition of the activation of p38MAPK, Ras/MEK/ERK and NF-κB signaling pathways. Thus, neoagarotetraose may attenuate the inflammatory responses through downregulating the MAPK and NF-κB signaling pathways in LPS-stimulated macrophages. In summary, the marine-derived neoagaro-oligosaccharide monomers merit further investigation as novel anti-inflammation agents in the future.
Regulation of striatal dopamine responsiveness by Notch/RBP-J signaling
Authors: Toritsuka M , Kimoto S , Muraki K , Kitagawa M , Kishimoto T , Sawa A , Tanigaki K 
Published: March 2017

AbstractDopamine signaling is essential for reward learning and fear-related learning, and thought to be involved in neuropsychiatric diseases. However, the molecular mechanisms underlying the regulation of dopamine responsiveness is unclear. Here we show the critical roles of Notch/RBP-J signaling in the regulation of dopamine responsiveness in the striatum. Notch/RBP-J signaling regulates various neural cell fate specification, and neuronal functions in the adult central nervous system. Conditional deletion of RBP-J specifically in neuronal cells causes enhanced response to apomorphine, a non-selective dopamine agonist, and SKF38393, a D1 agonist, and impaired dopamine-dependent instrumental avoidance learning, which is corrected by SCH23390, a D1 antagonist. RBP-J deficiency drastically reduced dopamine release in the striatum and caused a subtle decrease in the number of dopaminergic neurons. Lentivirus-mediated gene transfer experiments showed that RBP-J deficiency in the striatum was sufficient for these deficits. These findings demonstrated that Notch/RBP-J signaling regulates dopamine responsiveness in the striatum, which may explain the mechanism whereby Notch/RBP-J signaling affects an individual’s susceptibility to neuropsychiatric disease.
Nonadjacent Dependency Learning in Cantonese-Speaking Children With and Without a History of Specific Language Impairment
Authors: Iao Lai-Sang , Ng Lai Yan , Wong Anita Mei Yin , Lee Oi Ting 
Published: March 2017

Purpose
This study investigated nonadjacent dependency learning in Cantonese-speaking children with and without a history of specific language impairment (SLI) in an artificial linguistic context.

Method
Sixteen Cantonese-speaking children with a history of SLI and 16 Cantonese-speaking children with typical language development (TLD) were tested with a nonadjacent dependency learning task using artificial languages that mimic Cantonese.

Results
Children with TLD performed above chance and were able to discriminate between trained and untrained nonadjacent dependencies. However, children with a history of SLI performed at chance and were not able to differentiate trained versus untrained nonadjacent dependencies.

Conclusions
These findings, together with previous findings from English-speaking adults and adolescents with language impairments, suggest that individuals with atypical language development, regardless of age, diagnostic status, language, and culture, show difficulties in learning nonadjacent dependencies. This study provides evidence for early impairments to statistical learning in individuals with atypical language development.
Weaknesses in Lexical-Semantic Knowledge Among College Students With Specific Learning Disabilities: Evidence From a Semantic Fluency Task
Authors: Hall Jessica , McGregor Karla K. , Oleson Jacob 
Published: March 2017

Purpose
The purpose of this study is to determine whether deficits in executive function and lexical-semantic memory compromise the linguistic performance of young adults with specific learning disabilities (LD) enrolled in postsecondary studies.

Method

One hundred eighty-five students with LD (
n
= 53) or normal language development (ND,
n
= 132) named items in the categories
animals
and
food
for 1 minute for each category and completed tests of lexical-semantic knowledge and executive control of memory. Groups were compared on total names, mean cluster size, frequency of embedded clusters, frequency of cluster switches, and change in fluency over time. Secondary analyses of variability within the LD group were also conducted.

Results
The LD group was less fluent than the ND group. Within the LD group, lexical-semantic knowledge predicted semantic fluency and cluster size; executive control of memory predicted semantic fluency and cluster switches. The LD group produced smaller clusters and fewer embedded clusters than the ND group. Groups did not differ in switching or change over time.

Conclusions
Deficits in the lexical-semantic system associated with LD may persist into young adulthood, even among those who have managed their disability well enough to attend college. Lexical-semantic deficits are associated with compromised semantic fluency, and the two problems are more likely among students with more severe disabilities.
Willing interpreters and receivers: American alumni of the Japan exchange and teaching (JET) program
Authors: Metzgar Emily 
Published: March 2017

AbstractEstablished in 1987, the Japanese government’s Japan Exchange and Teaching (JET) Program has generated more than 60,000 alumni worldwide, half of them Americans. Coalescing over three decades, the American JET alumni community offers a compelling example of how the creation of “willing interpreters and receivers” (Nye) through an international exchange program can yield benefits for both sponsors and participants. Focused on the American JET alumni community’s composition and its efforts to organize into an independent actor in its own right, this paper offers insight into a large-scale effort to promote soft power in the context of the strategically important US—Japan relationship. This article is published as part of a collection on soft power.
Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors
Authors: Zhang Yu Shrike , Aleman Julio , Shin Su Ryon , Kilic Tugba , Kim Duckjin , Mousavi Shaegh Seyed Ali , Massa Solange , Riahi Reza , Chae Sukyoung , Hu Ning , Avci Huseyin , Zhang Weijia , Silvestri Antonia , Sanati Nezhad Amir , Manbohi Ahmad , De Ferrari Fabio , Polini Alessandro , Calzone Giovanni , Shaikh Noor , Alerasool Parissa , Budina Erica , Kang Jian , Bhise Nupura , Ribas João , Pourmand Adel , Skardal Aleksander , Shupe Thomas , Bishop Colin E. , Dokmeci Mehmet Remzi , Atala Anthony , Khademhosseini Ali 
Published: March 2017

Significance
Monitoring human organ-on-a-chip systems presents a significant challenge, where the capability of in situ continual monitoring of organ behaviors and their responses to pharmaceutical compounds over extended periods of time is critical in understanding the dynamics of drug effects and therefore accurate prediction of human organ reactions. In this work, we report a fully integrated modular physical, biochemical, and optical sensing platform, interfaced through a fluidics-routing breadboard with a multi–organ-on-a-chip system to achieve in situ, continual, and automated sensing of microenvironment biophysical and biochemical parameters. It is anticipated that our platform technology that is conveniently compatible with existing organ-on-a-chip models will potentially enhance their performance in drug screening by providing a multitude of sensing data not previously available.
Stepwise unfolding supports a subunit model for vertebrate kinetochores
Authors: Vargiu Giulia , Makarov Alexandr A. , Allan James , Fukagawa Tatsuo , Booth Daniel G. , Earnshaw William C. 
Published: March 2017

Significance
During cell division, microtubules apply piconewton forces to segregate duplicated chromosomes into daughter cells. The kinetochore, located on the surface of the centromere chromatin, couples microtubules to the chromosomes. Little is known about the folding of the centromeric chromatin and how this templates the functional ultrastructure of the kinetochore. To better understand this fundamental problem, we used a microscopy technique that allowed the DNA associated with centromeric chromatin to be unfolded and accurately measured in the presence and absence of several key kinetochore components. By combining this microscopy method with statistical analysis of the unfolded chromatin fibers, we acquired data that allowed a subunit model of the kinetochore chromatin to be proposed.
Anatomy of news consumption on Facebook
Authors: Schmidt Ana Lucía , Zollo Fabiana , Del Vicario Michela , Bessi Alessandro , Scala Antonio , Caldarelli Guido , Stanley H. Eugene , Quattrociocchi Walter 
Published: March 2017

Significance
Social media heavily changed the way we get informed and shape our opinions. Users’ polarization seems to dominate news consumption on Facebook. Through a massive analysis on 920 news outlets and 376 million users, we explore the anatomy of news consumption on Facebook on a global scale. We show that users tend to confine their attention on a limited set of pages, thus determining a sharp community structure among news outlets. Furthermore, our findings suggest that users have a more cosmopolitan perspective of the information space than news providers. We conclude with a simple model of selective exposure that well reproduces the observed connectivity patterns.
Hunter-gatherer residential mobility and the marginal value of rainforest patches
Authors: Venkataraman Vivek V. , Kraft Thomas S. , Dominy Nathaniel J. , Endicott Kirk M. 
Published: March 2017

SignificanceHunter-gatherers are notable for their high levels of mobility, but the ecological and social cues that determine the timing of camp movements (residential mobility) are poorly understood. Using models from foraging theory, we found that, for one population of hunter-gatherers, camp movements coincided with the point at which resource acquisition declined to a critical threshold level, but before local resources were completely depleted. These results suggest that hunter-gatherer residential mobility is constrained in a predictable fashion by rates of local resource depletion.
Myosin filament activation in the heart is tuned to the mechanical task
Authors: Reconditi Massimo , Caremani Marco , Pinzauti Francesca , Powers Joseph D. , Narayanan Theyencheri , Stienen Ger J. M. , Linari Marco , Lombardi Vincenzo , Piazzesi Gabriella 
Published: March 2017

Significance
This paper represents a major advance in understanding intrinsic heart beat regulation, because it provides an integrated view of the Frank–Starling law that combines mechanical, structural, and energetic aspects of heart performance. We show that mechanosensing in the myosin filament adjusts the number of myosin motors recruited from the off, ATP hydrolysis-unavailable state to the systolic force. In this way, the energetic cost of the heart beat is tuned to the end systolic pressure–volume relation. These results elucidate a plausible mechanistic link between the mutations that affect the structure of the myosin filament and cause hypercontractility and/or loss of contractile efficiency and dysregulation of the control by the stress-sensing mechanism on the state of the myosin motors.
Unified reduction principle for the evolution of mutation, migration, and recombination
Authors: Altenberg Lee , Liberman Uri , Feldman Marcus W. 
Published: March 2017

Significance
Evolution by Darwinian natural selection can not only shape how organisms survive and reproduce, but also affect transmission of genetic and other information between generations. Modifier-gene models for the evolution of information transmission have revealed a universal tendency for more faithful transmission to evolve in populations at equilibrium where natural selection is balanced by errors in information transmission. This is shown to be a very general property of models that include mutation and migration under selection and recombination under selection on diploids. The breadth of this reduction principle focuses attention on the departures from its mathematical assumptions, which may explain those biological phenomena of information transmission between generations for which the reduction principle fails.
Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers
Authors: Zhu Bin , Wang Longfei , Mitsunobu Hitoshi , Lu Xueling , Hernandez Alfredo J. , Yoshida-Takashima Yukari , Nunoura Takuro , Tabor Stanley , Richardson Charles C. 
Published: March 2017

Significance
Most DNA polymerases initiate DNA synthesis by extending a preexisting primer. Exceptions to this dogma are recently characterized bifunctional primase–polymerases (prim–pols) that resemble archaeal primases in their structure and initiate DNA synthesis de novo using only NTPs or dNTPs. We report here a DNA polymerase encoded by a phage NrS-1 from deep-sea vents. NrS-1 has a genome organization unlike any other known phage. Although this polymerase does not contain a zinc-binding motif typical for primases, it is nonetheless able to initiate DNA synthesis from a specific DNA sequence exclusively using dNTPs. Thus, it represents a unique de novo replicative DNA polymerase that possesses features found in DNA polymerases, primases, and RNA polymerases.
Metallacycle-cored supramolecular assemblies with tunable fluorescence including white-light emission
Authors: Zhang Mingming , Yin Shouchun , Zhang Jing , Zhou Zhixuan , Saha Manik Lal , Lu Chenjie , Stang Peter J. 
Published: March 2017

SignificanceLight-emitting materials have been widely studied for their promising applications in chemical and biological science. Here we report three phenanthrene-21-crown-7 functionalized fluorescent Pt(II) rhomboidal metallacycles which were then converted into fluorescent supramolecular oligomers by reacting with a bis-ammonium linker. One of these constructs shows concentration-dependent fluorescence in a wide color range, where orange emission at high concentration and blue emission at low concentration was observed. Moreover, at a concentration of 29 µM the same ensemble emits white light that has emerged from the integration of the complementary orange and blue color from the metallacycles and linker, respectively. This study shows how light-emitting materials can be obtained by the proper implementation of multiple orthogonal interactions in a single process.