bluuebunny/crossref_metadata_2025_split huggingface dataset:
NogoA Neutralization Promotes Axonal Restoration After White Matter Injury In Subcortical Stroke
Authors: Otero-Ortega Laura , Gómez-de Frutos Mari Carmen , Laso-García Fernando , Sánchez-Gonzalo Alba , Martínez-Arroyo Arturo , Díez-Tejedor Exuperio , Gutiérrez-Fernández María 
Published: August 2017

AbstractBlocking axonal growth inhibitor NogoA has been of great interest for promoting axonal recovery from neurological diseases. The present study investigates the therapeutic effects of blocking NogoA, inducing functional recovery and promoting white matter repair in an experimental animal model of stroke. Adult male rats were subjected to white matter injury by subcortical ischemic stroke. Twenty-four hours after surgery, 250 ug of anti-NogoA or anti-IgG-1 were administered through the tail vein. The quantity of NogoA protein was determined by immunohistochemistry in the brain and peripheral organs. In addition, functional status, lesion size, fiber tract integrity, axonal sprouting and white matter repair markers were analyzed. Moreover, an in vitro study was performed in order to strengthen the results obtained in vivo. A lower quantity of NogoA protein was found in the brain and peripheral organs of the animals that received anti-NogoA treatment. The animals receiving anti-NogoA treatment showed significantly better results in terms of functional recovery, fiber tract integrity, axonal sprouting and white matter repair markers compared with the control group at 28 days. White matter integrity was in part restored by antibody-mediated inhibition of NogoA administration in those animals that were subjected to an axonal injury by subcortical stroke. This white matter restoration triggered functional recovery.
Sofosbuvir protects Zika virus-infected mice from mortality, preventing short- and long-term sequelae
Authors: Ferreira André C. , Zaverucha-do-Valle Camila , Reis Patrícia A. , Barbosa-Lima Giselle , Vieira Yasmine Rangel , Mattos Mayara , Silva Priscila de Paiva , Sacramento Carolina , de Castro Faria Neto Hugo C. , Campanati Loraine , Tanuri Amilcar , Brüning Karin , Bozza Fernando A. , Bozza Patrícia T. , Souza Thiago Moreno L. 
Published: August 2017

AbstractZika virus (ZIKV) causes significant public health concerns because of its association with congenital malformations, neurological disorders in adults, and, more recently, death. Considering the necessity to mitigate ZIKV-associated diseases, antiviral interventions are an urgent necessity. Sofosbuvir, a drug in clinical use against hepatitis C virus (HCV), is among the FDA-approved substances endowed with anti-ZIKV activity. In this work, we further investigated the in vivo activity of sofosbuvir against ZIKV. Neonatal Swiss mice were infected with ZIKV (2 × 107 PFU) and treated with sofosbuvir at 20 mg/kg/day, a concentration compatible with pre-clinical development of this drug. We found that sofosbuvir reduced acute levels of ZIKV from 60 to 90% in different anatomical compartments, such as the blood plasma, spleen, kidney, and brain. Early treatment with sofosbuvir doubled the percentage and time of survival of ZIKV-infected animals. Sofosbuvir also prevented the acute neuromotor impairment triggered by ZIKV. In the long-term behavioural analysis of ZIKV-associated sequelae, sofosbuvir prevented loss of hippocampal- and amygdala-dependent memory. Our results indicate that sofosbuvir inhibits ZIKV replication in vivo, which is consistent with the prospective necessity of antiviral drugs to treat ZIKV-infected individuals.
Umbilical cord-derived mesenchymal stem cell extracts reduce colitis in mice by re-polarizing intestinal macrophages
Authors: Song Ji-young , Kang Hyo Jeong , Hong Joon Seok , Kim Chong Jai , Shim Jae-Yoon , Lee Christopher W. , Choi Jene 
Published: August 2017

AbstractHuman umbilical cord mesenchymal stem cells (hUC-MSCs), originating in Wharton’s jelly, are multipotent stem cells that home to damaged tissues and can modulate the immune system. We examined whether administering extracts of MSCs (MSC-Ex) instead of MSCs could augment the beneficial effects of MSC therapy by overcoming the low homing efficiency of MSCs systemically administered in inflammatory bowel diseases (IBD). Dextran sodium sulfate-induced colitis model was established in C57BL/6 mice, and MSC-Ex was administered intraperitoneally. MSC-Ex reduced colitis, disease activity index (DAI), and histological colitis scores, and increased the body weight. Treatment with MSC-Ex completely blocked the induction of inflammatory cytokines, which were strongly detected in mice with colitis. MSC-Ex shifted the macrophage functional phenotype from M1 to M2 by decreasing the levels of MCP1, CXCL9, and iNOS, but increasing the levels of IL-10, LIGHT, CCL1, and Arg-1. MSC-Ex recovered the destruction of the epithelial barrier in the differentiated Caco-2 cells in vitro. Treatment with MSC-Ex was more potent than that with MSC in reducing DAI, the histological score, and nitrite levels. These data strongly support that MSC-Ex treatment can be a potent approach to overcome severe refractory IBD.
Fluorescent organic single crystals with elastic bending flexibility: 1,4-bis(thien-2-yl)-2,3,5,6-tetrafluorobenzene derivatives
Authors: Hayashi Shotaro , Asano Atsushi , Kamiya Natsumi , Yokomori Yoshinobu , Maeda Takuto , Koizumi Toshio 
Published: August 2017

AbstractOrganic single crystals with elastic bending flexibility are rare because they are generally brittle. We report here fluorescent organic single crystals based on thiophene-tetrafluorobenzene-thiophene derivatives, mainly 1,4-bis(thien-2-yl)-2,3,5,6-tetrafluorobenzene. Three derivatives were synthesized by Pd-catalyzed cross-coupling reactions (Stille or direct arylation pathways). The crystallization of the derivatives gave large (mm- or cm-scale) crystals. Two crystals of 1,4-bis(thien-2-yl)-2,3,5,6-tetrafluorobenzene, 1, and 1,4-bis(4-methylthien-2-yl)-2,3,5,6-tetrafluorobenzene, 3, bent under applied stress and quickly recovered its original shape upon relaxation. The other crystal of 1,4-bis(5-methylthien-2-yl)-2,3,5,6-tetrafluorobenzene, 2, showed brittle breakage under applied stress (normal behavior). Fibril lamella crystal structure based on criss-cross packed slip-stacked molecular wires and its structural integrity are important factors for the design and production of next generation crystal materials with elastic bending flexibility. Furthermore, mechanical bending–relaxation resulted in reversible change of the morphology and fluorescence (mechanofluorochromism). Such bendable crystals would lead to the next generation solid-state fluorescent and/or semiconducting materials.
Simultaneous Gene Delivery and Tracking through Preparation of Photo-Luminescent Nanoparticles Based on Graphene Quantum Dots and Chimeric Peptides
Authors: Ghafary Soroush Moasses , Nikkhah Maryam , Hatamie Shadie , Hosseinkhani Saman 
Published: August 2017

AbstractDesigning suitable nano-carriers for simultaneous gene delivery and tracking is in the research priorities of the molecular medicine. Non-toxic graphene quantum dots (GQDs) with two different (green and red) emission colors are synthesized by Hummer’s method and characterized by UV-Vis, Photoluminescence (PL), Fourier Transform Infrared (FTIR) and Raman spectroscopies, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The GQDs are conjugated with MPG-2H1 chimeric peptide and plasmid DNA (pDNA) by non-covalent interactions. Following conjugation, the average diameter of the prepared GQDs increased from 80 nm to 280 nm in complex structure, and the ζ-potential of the complex increased (from −36.87 to −2.56 mV). High transfection efficiency of the nano-carrier and results of confocal microscopy demonstrated that our construct can be considered as a nontoxic carrier with dual functions for gene delivery and nuclear targeting.
Strong electric wave response derived from the hybrid of lotus roots-like composites with tunable permittivity
Authors: Liang Xiaohui , Quan Bin , Chen Jiabin , Tang Dongming , Zhang Baoshan , Ji Guangbin 
Published: August 2017

AbstractLotus roots-like NiO/NiCo2O4 hybrids derived from Metal-organic frameworks (MOFs) are fabricated for the first time by using flake NiCo-MOF precursors as reactant templates. It was found that a thin sample consisting of 60 wt % NiO/NiCo2O4 hybrids in the wax matrix exhibited an effective microwave absorption bandwidth of 4.2 GHz at the thickness of 1.6 mm. The highest reflection loss of −47 dB was observed at 13.4 GHz for a sample with a thickness of 1.7 mm. Results obtained in this study indicate that hybrids of NiO and NiCo2O4 are promising microwave absorbing materials with adjustable permittivity, which can exhibit broad effective absorption bandwidth at low filler loading and thin thickness.
In situ repair of bone and cartilage defects using 3D scanning and 3D printing
Authors: Li Lan , Yu Fei , Shi Jianping , Shen Sheng , Teng Huajian , Yang Jiquan , Wang Xingsong , Jiang Qing 
Published: August 2017

AbstractThree-dimensional (3D) printing is a rapidly emerging technology that promises to transform tissue engineering into a commercially successful biomedical industry. However, the use of robotic bioprinters alone is not sufficient for disease treatment. This study aimed to report the combined application of 3D scanning and 3D printing for treating bone and cartilage defects. Three different kinds of defect models were created to mimic three orthopedic diseases: large segmental defects of long bones, free-form fracture of femoral condyle, and International Cartilage Repair Society grade IV chondral lesion. Feasibility of in situ 3D bioprinting for these diseases was explored. The 3D digital models of samples with defects and corresponding healthy parts were obtained using high-resolution 3D scanning. The Boolean operation was used to achieve the shape of the defects, and then the target geometries were imported in a 3D bioprinter. Two kinds of photopolymerized hydrogels were synthesized as bioinks. Finally, the defects of bone and cartilage were restored perfectly in situ using 3D bioprinting. The results of this study suggested that 3D scanning and 3D bioprinting could provide another strategy for tissue engineering and regenerative medicine.
Superior Electronic Structure in Two-Dimensional MnPSe
3

/MoS2 van der Waals Heterostructures
Authors: Pei Qi , Song Yan , Wang Xiaocha , Zou Jijun , Mi Wenbo 
Published: August 2017

AbstractWe explore the electronic structure of two-dimensional (2D) MnPSe3/MoS2 van der Waals (vdW) heterostructures based on density functional theory. A novel spin splitting at the valance band maximum of MnPSe3 appears in some specific stacking models due to Mn d orbital hybridization. The simultaneous spin and valley splitting can be achieved by interfacial coupling, which is attractive for manipulation of the valley and spin degrees of freedom. More importantly, due to the antiferromagnetic ordering of manganese, the opposite spin moments at K and K′ valleys can be observed by transforming configurations, which realizes the tunable spin splitting states. Our theoretical work opens up the opportunities of valley and spin related applications of MnPSe3/MoS2 vdW heterostructures and offers a practical avenue for exploring novel devices based on the spin and valley degrees of freedom.
The Parkinson’s disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo
Authors: Morató Xavier , Luján Rafael , López-Cano Marc , Gandía Jorge , Stagljar Igor , Watanabe Masahiko , Cunha Rodrigo A. , Fernández-Dueñas Víctor , Ciruela Francisco 
Published: August 2017

AbstractG protein-coupled receptor 37 (GPR37) is an orphan receptor associated to Parkinson’s disease (PD) neuropathology. Here, we identified GPR37 as an inhibitor of adenosine A2Areceptor (A2AR) cell surface expression and functionin vivo. In addition, we showed that GPR37 and A2AR do oligomerize in the striatum. Thus, a close proximity of GPR37 and A2AR at the postsynaptic level of striatal synapses was observed by double-labelling post-embedding immunogold detection. Indeed, the direct receptor-receptor interaction was further substantiated by proximity ligationin situassay. Interestingly, GPR37 deletion promoted striatal A2AR cell surface expression that correlated well with an increased A2AR agonist-mediated cAMP accumulation, both in primary striatal neurons and nerve terminals. Furthermore, GPR37−/− mice showed enhanced A2AR agonist-induced catalepsy and an increased response to A2AR antagonist-mediated locomotor activity. Overall, these results revealed a key role for GPR37 controlling A2AR biology in the striatum, which may be relevant for PD management.
Structure and magnetic properties of icosahedral PdxAg13−x (x = 0–13) clusters
Authors: Fan Bai , Ge Gui-Xian , Jiang Cheng-Huan , Wang Guang-Hou , Wan Jian-guo 
Published: August 2017

AbstractIn this article, we present a modified Velocity-Verlet algorithm that makes cluster system converge rapidly and accurately. By combining it with molecular dynamics simulations, we develop an effective global sampling method for extracting isomers of bimetallic clusters. Using this method, we obtain the isomers of icosahedral PdxAg13−x (x = 0–13). Additionally, using the first-principle spin-polarized density functional theory approach, we find that each isomer still retains its icosahedral structure because of strong s-d orbital hybridization, and the cluster is more stable when a Pd atom is at the center of the cluster. With increasing x value, the magnetic moment decreases linearly from 5.0 μB at x = 0, until reaching zero at x = 5, and then increases linearly up to 8.0 μB at x = 13. By calculating the atom-projected density of states (PDOS), we reveal that the magnetic moment of PdxAg13−x mainly originates from s electrons of Ag when 0 ≤ x < 5, and d electrons of Pd when 5 < x ≤ 13. The PDOS results also show that the PdxAg13−x tends to transform from a semiconductor state to semi-metallic state when x gradually increases from 0 to 13.
Poly-Methyl Methacrylate/Polyvinyl Alcohol Copolymer Agents Applied on Diabetic Wound Dressing
Authors: Hsieh Hsiao-Ting , Chang Hung-Ming , Lin Wei-Jhih , Hsu Yao-Tsung , Mai Fu-Der 
Published: August 2017

AbstractDue to the difficulty of healing chronic wound, in the process of changing dressing, secondary damage on the tissue caused by adhesion should be prevented. In this study, the new dressing of particle hydrogels synthesized with poly-methyl methacrylate and poly-vinyl alcohol precursors were proposed. In addition, cell safety tests, animal’s allergic stimulation, and animal’s wound healing experiments were conducted for particle hydrogels. On one hand, in L929 cell experiment, the results of particle hydrogels extract 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide tests and lactate dehydrogenase test trial show that there are no safety concerns over particle hydrogels. On the other hand, New Zealand white rabbits were chosen for skin sensitization tests in animal trials, which show the consistent results. At last, wound healing tests used diabetes induction with 10-week-old rats and three-month-old Landrace pigs, with the tissue histology. In short, through this experiment, it is found that in the early phase of the diabetic rats and pigs’ wound healing, using particle hydrogels can enhance collagen formation, and achieve the goal of faster wound healing.
Relationship between initiation time of adjuvant chemotherapy and survival in ovarian cancer patients: a dose-response meta-analysis of cohort studies
Authors: Liu Yi , Zhang Tiening , Wu Qijun , Jiao Yisheng , Gong Tingting , Ma Xiaoxin , Li Da 
Published: August 2017

AbstractAlthough several studies have previously investigated the association between the initiation time of adjuvant chemotherapy and survival in ovarian cancer, inconsistencies remain about the issue. We searched PubMed and Web of Science through the May 24, 2017 to identify cohort studies that investigated the aforementioned topic. Fourteen studies with 59,569 ovarian cancer patients were included in this meta-analysis. We conducted meta-analyses comparing the longest and shortest initiation time of adjuvant chemotherapy and dose-response analyses to estimate summary hazards ratios (HRs) and 95% confidence intervals (CIs). A random-effects model was used to estimate HRs with 95% CIs. When comparing the longest with the shortest category of initiation time of adjuvant chemotherapy, the summary HR was 1.18 (95% CI: 1.06–1.32; I2 = 17.6; n = 7) for overall survival. Additionally, significant dose-response association for overall survival was observed for each week delay (HR = 1.04; 95% CI: 1.00–1.09; I2 = 9.05; n = 5). Notably, these findings were robust in prospective designed cohort studies as well as studies with advanced stage (FIGO III-IV) patients. No evidence of publication bias was observed. In conclusion, prolonged initiation time of adjuvant chemotherapy is associated with a decreased overall survival rate of ovarian cancer, especially in patients with advanced stage ovarian cancer.
Sonic Hedgehog mimetic prevents leukocyte infiltration into the CNS during acute HIV infection
Authors: Singh Vir B. , Singh Meera V. , Piekna-Przybylska Dorota , Gorantla Santhi , Poluektova Larisa Y. , Maggirwar Sanjay B. 
Published: August 2017

AbstractInfiltration of infected leukocytes culminates in establishment of a brain niche for Human Immunodeficiency Virus (HIV) during acute phase of infection, initiating an ongoing cascade of persistent viral replication and inflammation, that causes irreversible neuronal injury and HIV associated neurocognitive disease (HAND). In this study, humanized mice were treated with Smoothened Agonist (SAG), a Sonic Hedgehog (Shh) mimetic in order to fortify blood brain barrier (BBB) and dampen leukocyte extravasation into CNS during AHI. Results indicate that SAG treatment reduced viral burden in the CNS immediately after HIV transmission, but also conferred extended neuroprotection via increased BBB integrity (elevated levels of tight-junction protein, Claudin 5, and reduced S100B levels in periphery). These mice also showed healthier neurons with thick, uniform dendrites and reduced numbers of activated astrocytes. Additional in vitro experiments suggested SAG treatment was not associated with the establishment or reversal of latency in the target cells. Altogether, these findings validate neuroprotective role of Shh signaling and highlight the therapeutic potential of Shh mimetics against CNS complications associated with HIV infection. Further our results strongly demonstrate that pharmacological interventions to reduce leukocyte mobilization during early HIV infection, can provide prolonged neuroprotection, which might significantly delay the onset of HAND.
Marked seasonality and high spatial variation in estuarine ciliates are driven by exchanges between the ‘abundant’ and ‘intermediate’ biospheres
Authors: Sun Ping , Huang Liying , Xu Dapeng , Huang Bangqin , Chen Nengwang , Warren Alan 
Published: August 2017

AbstractWe examined the spatial and temporal variability of ciliate community in a subtropical estuary by rRNA and rDNA-based high throughput sequencing of 97 samples collected along the entire salinity gradient at two-month intervals in 2014. Community divided statistically into three groups: freshwater (salinity < 0.5‰), oligohaline and mesohaline (0.5‰ < salinity < 18‰), and polyhaline and euhaline (18‰ < salinity < 40‰). Across all three groups, salinity explained most of the community variability. Within each group, seasonal shifts in community formed cool (spring and winter) and warm (summer and autumn) subgroups, indicating that spatial variability overrode seasonal changes in determining community composition. Cool and warm groups showed opposite associations with temperature and prey proxies, suggesting distinct seasonal niche separation. The community reassembly of cool and warm groups was essentially due to transitions between intermediate (with relative abundance of 0.01–1%) and abundant (with relative abundance > 1%) OTUs. Further analyses demonstrated that the intermediate group not only encompassed comparable OTU richness to that of the total community and maintained high metabolic activity but also had the highest proportion in transition, either to abundance or rarity, thus offering a first view on how it varies across space and time and revealing the essential role it played in maintaining stability and functionality within the community.
Adhesion to stromal cells mediates imatinib resistance in chronic myeloid leukemia through ERK and BMP signaling pathways
Authors: Kumar Atul , Bhattacharyya Jina , Jaganathan Bithiah Grace 
Published: August 2017

AbstractChronic myeloid leukemia (CML) is characterized by abnormal proliferation of myeloid cells which when untreated leads to bone marrow failure. Imatinib mesylate (IM) is the first line of therapy for treatment of CML and results in remission in most cases. However, a significant percentage of patients develop chemoresistance to IM, which might be due to the presence of chemoresistant cells in the bone marrow. In the current study, we explored the role of cell-cell interaction of CML cells with the bone marrow stromal cells in the development of chemoresistance in CML. We found that the stromal cells offered long-term chemoprotection to the CML cells from the apoptotic effect of IM. These stroma interacting CML cells were maintained in a non-proliferative stage and had increased ERK1/2 and SMAD1/8 phosphorylation levels. Prolonged interaction of CML cells with the stromal cells in the presence of IM resulted in the acquisition of stroma-free chemoresistance to IM treatment. However, inhibition of actin cytoskeleton, ERK1/2 and SMAD signaling abrogated the chemoresistance acquisition and sensitized the chemoresistant CML cells to IM induced apoptosis.
Nonlocal Effects and Slip Heat Flow in Nanolayers
Authors: Zhu Chuan-Yong , You Wei , Li Zeng-Yao 
Published: August 2017

AbstractGuyer-Krumhansl (G-K) equation is a promising macroscopic model to explore heat transport in nanoscale. In the present work, a new nonlocal characteristic length is proposed by considering the effects of heat carriers-boundaries interactions to modify the nonlocal term in G-K equation, and a slip heat flux boundary condition is developed based on the local mean free path of heat carriers. Then an analytical solution for heat flux across 2-D nanolayers and an in-plane thermal conductivity model are obtained based on the modified G-K equation and the slip heat flux boundary. The predictions of the present work are in good agreement with our numerical results of direct simulation Monte Carlo (DSMC) for argon gas nanolayer and the available experimental data for silicon thin layers. The results of this work may provide theoretical support for actual applications of G-K equation in predicting the thermal transport properties of nanolayers.