bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression Level of Latent Membrane Protein 1 Epstein-Barr Virus in Tissue Biopsy Among Different Clinical Stages of Nasopharyngeal Carcinoma
Authors: Purwono Priyo Budi , Romdhoni Achmad Chusnu , Rahaju Anny Setijo , Rahniayu Alphania , Astutik Anis Fidiah , Dewayani Astri , Rachmadhan Hayyu Fath , Ashari Faisal Yusuf , Wiqoyah Nurul , Purwandari Chatarina Anugrah Ambar 
Published: October 2024

Abstract

Background
Nasopharyngeal carcinoma (NPC) is a prevalent malignancy in the head and neck area, particularly affecting males. NPC is consistently linked to Epstein-Barr virus (EBV) infection, environmental and genetic factors. EBV is an oncogenic herpes virus that harbours the latent membrane protein (LMP1). This study aimed to analyze the association between the expression level of LMP1 and clinical stages of NPC.
Methods
A retrospective study of formalin-fixed, paraffin-embedded (FFPE) tissues of NPC was examined by immunohistochemistry (IHC). Additionally, semi-quantitative scoring was performed to determine the degree of LMP1 expression. The difference between LMP1 expression and the clinical stage was analyzed through Kruskal-Wallis statistics.
Results
Our study revealed that the prevalence of EBV-LMP1 in NPC was 98% (78/79). The expression of LMP1 was the most detected in 93.7% of non-keratinizing undifferentiated and 6.3% of non-keratinizing carcinoma. The prevalence of LMP1-positive patients distributed in clinical stages II, III, and IV was 8.8%, 6.5%, and 74.7%, respectively. The percentage of LMP1 +++ expression for clinical stages II, III, and IV are 14.29%, 23.08%, and 50.85%, respectively. No significant difference was observed in the expression of LMP1 across different clinical stages.
Conclusions
The prevalence of Epstein-Barr virus infection is relatively high in nasopharyngeal cancer, namely in the non-keratinizing undifferentiated type. There is an elevated expression of LMP1 in higher clinical stages of NPC; however, it may not correlate. Therefore, further study in a larger population is worth conducting.
Rationale and design of Healthy Kids Beyond the Bell: A 2x2 full factorial study evaluating the impact of summer and after school programming on children’s body mass index and health behaviors.
Authors: Weaver R Glenn , Beets Michael W , Adams Elizabeth L , Kaczynski Andrew T , Chen Brian , Armstrong Bridget , Burkart Sarah , Kiely Keagan , Cepni Aliye B. , III James W White , Finnegan Olivia , Savidge Meghan , Parker Hannah , Randolph Griffin AT 
Published: October 2024

Abstract

Background. The structured days hypothesis posits that structure protects children against obesogenic behaviors (e.g., physical inactivity, unhealthy dietary intake), and, ultimately, prevents the occurrence of excessive weight gain. The hours following school (i.e., 3-6pm school days) and summer vacation are two "windows of vulnerability" when children may experience less structure. Programs that provide a healthy structured environment and may prevent BMI gain exist for both time periods (i.e., after school programs and summer day camps). Unfortunately, these programs are cost prohibitive for children from low-income families to attend. This study will test the impact of providing vouchers to access existing, community-operated after school and summer programs on BMI z-score, body composition, and obesogenic behaviors (i.e., physical activity, screen use, diet, and sleep) of children (5–12 years) from schools that primarily serve families with low-income.Methods. The study will employ a 2x2 factorial design. Participants (N = 480) attending 4 elementary schools in one school district will be randomly assigned to a no treatment control, after school program voucher only, summer day camp voucher only, or after school and summer day camp vouchers. Vouchers will cover the full cost of attending a pre-existing community-based after school or summer camp program. The primary outcome (BMI z-score) will be measured at baseline (before end of school year, ~May), 3-month follow-up (after summer, ~August), and 12-month follow-up (end the following school year, ~May). Secondary outcomes include body composition (i.e., whole-body fat mass, fat free mass, and percent body fat) and obesogenic behaviors (i.e., physical activity, sedentary time, sleep, screen-time, and diet). The study will also employ a rigorous process evaluation which will consider after school and summer camp program attendance and content. Analyses will examine differences between the four groups in BMI z-score, body composition, and obesogenic behaviors. Incremental cost effectiveness ratios will determine the cost effectiveness of the intervention.Discussion. The current study will provide critical information for researchers, practitioners, and policy makers seeking to combat the childhood obesity epidemic in children from families with low-income during the school year and summer.Trial Registration NCT05880901. Registered 27 May 2023. https://clinicaltrials.gov/study/NCT05880901?titles=beyond%20the%20bell&rank=1
Effects of the hormone replacement therapy on arterial stiffness and hemodynamics in the perimenopausal women
Authors: zhou yi , chen xiao guo , huang yu , wang shan , yin li xue 
Published: October 2024

Abstract

Objective
This study aimed to examine the effect of hormone replacement therapy on arterial stiffness and hemodynamics in the perimenopausal women.
Methods
Sixty perimenopausal women were recruited for the present cohort study among the patients visiting our menopause clinic. 30 women, who were prescribed to do the hormone replacement therapy (HRT) from several months to 1 year, were investigated, together with another age-matched group (Control group) of 30 perimenopausal women without HRT. All participants underwent 2 serial carotid pulse wave velocity (PWV) and wall shear stress (WSS) assessments of the carotid artery at 1-year interval.
Results
Compared with the baseline value, there was no significant difference in PWV over 12 months between HRT and Control group, however, the WSS value increased significantly for both groups in 1 year (p < 0.05). After 1 year of the follow-up, the PWV at BS value was significantly lower in the HRT group than in the Control one (p < 0.05), but there was no significant statistical difference in the WSS value between two groups. Multiple linear regression analyses revealed that Triglycerides and HDL cholesterol were independently correlated with the changes in PWV (p < 0.05).
Conclusions
The hormone replacement therapy appears to impact on the arterial stiffness without affecting hemodynamics in the perimenopausal women. The time-mediated influence on WSS seems to overrule the potential HRT-mediated influence, thus the change of WSS cannot be excluded. Further research is needed to understand clearly the mechanism underlying the obtained results.
Association between Life’s Essential 8 and abdominal aortic calcification: findings from a national study
Authors: Xu Jun-Peng , Jin Li , Peng Xiong-Qiang , Zhao Xu-Jie , Guo Li-Heng , Mai Xiao-Yi , Zeng Rui-Xiang , Zhang Min-Zhou 
Published: October 2024

Abstract

Background and aims
Data are lacking regarding Life’s Essential 8 (LE8), a cardiovascular health (CVH) scoring system recently updated by the American Heart Association, and abdominal aortic calcification (AAC). We aimed to detect the associations of total and individual CVH metrics based on LE8 with AAC-score and AAC prevalence.
Methods
We conducted a cross-sectional study from the National Health and Nutrition Examination Survey (NHANES) 2013–2014. Individuals with a score exceeding 0 or 6 were considered as having AAC and severe AAC, respectively. Total and individual CVH metric scores were stratified into low level (0–49), intermediate level (50–74), and high level (75–100). Multivariable linear or logistic regression models were performed to calculate β or odds ratios (ORs) and corresponding 95% confidence interval (CI) for the associations of total and individual CVH metrics with AAC. For further exploration, restricted cubic spline curves and subgroup analyses were performed.
Results
2,403 eligible participants were finally included. Compared to those with a low total CVH score, adults with a high total CVH score were associated with lower AAC-score [β: -0.50, (-0.95, -0.06), P = 0.0277] and reduced prevalence of AAC (OR: 0.57, (0.41, 0.80), P = 0.0013] and severe AAC [OR: 0.52, (0.30, 0.90), P = 0.0197]. There was a nonlinear and linear dose–response inverse associations of total CVH score with AAC and severe AAC, respectively. For the eight individual CVH metrics, well management of nicotine exposure, blood glucose and blood pressure was the major individual contributor to prevent AAC. Equally, enough engagement of physical activity could partly reduce AAC risk.
Conclusions
The ideal CVH assessed by Life’s Essential 8 metrics was significantly associated with reduced AAC risk. We emphasize that earlier efforts on promoting CVH metrics should be highlighted to reduce AAC prevalence and subsequent CVD in later life.
The Impact of Big Data Analytics on Health Care: A Systematic Review
Authors: Akour Iman , Salloum Said 
Published: October 2024

Abstract

The extension of Big Data analytics to healthcare has radically altered how healthcare-related data is managed and used, presenting unequalled chances of augmenting patient experiences, boosting operational effectiveness, and customising treatment regimens. The prospective advantages of Big Data in medical fields have been ameliorated to a larger extent due to recent technological breakthroughs. Regardless of the noteworthy progress, an in-depth comprehension of the exact manner in which Big Data analytics improves numerous healthcare applications is lacking. The abrupt advancement of these technological innovations and their utilisation in the healthcare sector demands a revised amalgamation of the existing research patterns, approaches, and outcomes. In order to answer six particular research questions on Big Data analytics in healthcare, this comprehensive analysis examined 127 research articles that were released between 2015 and 2024. The evaluation used a methodically organised approach that included determining the parameters for inclusion and exclusion, data sources, search tactics, quality evaluation, and data coding and analysis. To facilitate a thorough and honest review procedure, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards were applied. According to the research, the fields with the highest representation in the literary works include sleep apnoea monitoring, digital health records, and BDA healthcare applications and platforms. The machine learning algorithms that are most commonly used are supervised learning approaches, including Linear Regression and Support Vector Machines. A geographic study showed that China, India, and the United States have made substantial contributions. The temporal study revealed a substantial spike in research endeavours between 2020 and 2023, indicating heightened interest in the fusion of Big Data with the healthcare industry. The year 2024 witnessed an abrupt reduction in publications, indicating either a level of saturation or a shift in the focus of the study. The results highlight how Big Data analytics may redefine healthcare by improving operational effectiveness, individualised treatment regimens, and diagnostic accuracy. The report also emphasises the significance of having strong ethical standards and legal frameworks in place in order to cope with data security and privacy issues. Future studies should concentrate on investigating upcoming technologies, multidisciplinary approaches, flawless integration with current systems, and the lasting effects of these technologies. Furthermore, encouraging international cooperation can improve the exchange of resources and ideal practices, expanding the scope of Big Data healthcare analytics globally.
Efficacy of CRISPR-Cas9 Gene Editing for Targeting KRAS Mutations in Pancreatic Cancer: A systematic review
Authors: Hashim Hashim Talib , Alhatemi Ahmed Qasim Mohammed , Fatima Tehreem , Haque Tahmina , Sulaiman Fatimah Abdullah , Musa Mohammed Ganim , Abdullah Muhanned Ahmed , Kadhum Rawaa Awad , Al-Obaidi Ahmed Dheyaa , Al-Obaidi Ammar 
Published: October 2024

Abstract

Background
Pancreatic cancer is one of the main causes of cancer-related deaths, especially pancreatic ductal adenocarcinoma and it’s characterized by a poor prognosis. The KRAS gene mutation is prevalent in about 85% of pancreatic cancer cases which is a significant factor in the pathogenesis and development of pancreatic cancer, impacting cellular tumor growth, survival, and metastasis. The targeted disruption of mutant KRAS variants through the application of various CRISPR systems has led to a marked reduction in cell viability and proliferation in vitro, accompanied by significant inhibition of tumor growth in vivo.
Method
This systematic study was adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and involved a comprehensive search of PubMed, Google Scholar, and PLOS One for original research articles published up to June 2024. Studies included those involving CRISPR-Cas9 gene editing targeting KRAS mutations in human or animal models of pancreatic cancer. Data collection and quality assessment were performed independently by two reviewers.
Results
The review identified numerous studies demonstrating the efficacy of CRISPR-Cas9 in targeting KRAS mutations. Results showed significant reductions in KRAS transcript levels, decreased tumor progression, and improved survival rates in experimental models. However, challenges such as off-target effects and efficient delivery methods were noted.
Conclusion
CRISPR-Cas9 gene editing shows significant promise as a therapeutic strategy for targeting KRAS mutations in pancreatic cancer. While the technology has demonstrated potential in preclinical studies, further research is needed to address challenges related to specificity, delivery, and long-term effects to facilitate its clinical application.
Surgical management of multiple damages of superior shoulder suspensory complex disruptions with coracoid fracture
Authors: Sun Guohai , Han Shumei , Jia Honglei , Pang Hongqian , Zhang Wupeng , Liu Fanxiao , Li Shanxin , Feng Minghao , Wang Zhuang , Wang Bomin , Yang Yongliang 
Published: October 2024

Abstract

Background
Multiple damages of the superior shoulder suspensory complex (SSSC) with coracoid fracture are exceedingly rare. Two or more site injuries of the SSSC frequently leads to delayed union, non-union and suffered motor dysfunction. Currently, the optimal surgical protocol has not been determined. This article reports our therapy approaches and clinical outcomes.
Methods
From January 2020 to June 2022. We treated 9 patients who had at least 3 injuries of SSSC and all with coracoid fracture. None of the patients had sustained concomitant injuries such as brachial plexus injury or rib fracture. All patients were treated with open reduction and internal fixation and coracoid fractures were reduced and fixed. The Visual Analgesia Score (VAS), American Shoulder and Elbow Surgeons (ASES) Score and Range of Motion (ROM) score were used to evaluation of postoperative recovery at the time of the last follow-up.
Conclusion
In conclusion, the manifestations of multiple SSSC disruptions with coracoid fracture are variable. A variety of surgical modalities for treating such injuries have been reported in the previous literature. In this article we introduce a novel, reliable surgical technique to treat such injuries.
Degree of coincidence of the change in health-related quality of life in the recovery-phase rehabilitation ward and the proxy’s response: Evaluation of patients with musculoskeletal disorder by EQ-5D-5L
Authors: Izumi Ryota , Noto Shinichi , Nagayama Hirofumi , Sano Tetsuya , Takizawa Hirokazu , Tsukakoshi Daichi 
Published: October 2024

Abstract

Background
Recently, a conversion table of the EuroQol 5-dimension 5-level (EQ-5D-5L) unique to Japan was created that facilitates the measurement of the health-related quality of life (HRQOL) of a participant. Currently, however, only a few investigations have been conducted of HRQOL using the EQ-5D-5L in the recovery-phase rehabilitation ward. Moreover, proxy responses for patients who are unable to respond for themselves have only been reported for stroke patients.
Methods
In this study, we used the EQ-5D-5L to investigate changes in HRQOL before and after rehabilitation and the degree of agreement between the patient’s responses and the occupational therapist’s proxy responses in patients with musculoskeletal disorders who were hospitalized in a recovery-phase rehabilitation ward.
Results
Among the 77 participants, HRQOL was improved after rehabilitation, and the effect size was large (self response; SRM = 0.90, proxy response; SRM = 0.95). Regarding the degree of agreement between the QOL values, the intraclass correlation coefficient was 0.68 for the initial evaluation and 0.72 for the re-evaluation, indicating substantial agreement.
Conclusions
The results of this study showed that the EQ-5D-5L was responsive as an indicator of outcome of patients with musculoskeletal disorders who were hospitalized in a recovery-phase rehabilitation ward and that proxy responses by occupational therapists can measure QOL values similar to those of the individuals themselves.
Viral load suppression after enhanced adherence counseling and its associated factors among clients on antiretroviral therapy
Authors: Bekele Teshome Nadew , Eshete Akine , Gebrie Abiraraw , Mekuria Abinet Dagnew , Dejene Tadesse Mamo 
Published: October 2024

Abstract

Background
Maintaining long-term client adherence and viral suppression requires ongoing increased adherence counseling and follow-up care. Promoting enhanced adherence counseling (EAC) is advised for those whose viral load is greater than or equivalent to 1000 copies/ml. However, there is still a lack of knowledge in the study region about the degree of viral load suppression that occurs after enhanced adherence counseling is implemented.
Objective
The study was to determine the magnitude of Viral load suppression after enhanced adherence counseling and its associated factors among Clients on Anti Retro Viral Therapy follow up in public health facilities in Debre Berhan Regio-Politian City, North Shewa, Ethiopia.
Methods
From July 2018 to June 2023, an institutionally based retrospective follow-up research was conducted. A total of 409 HIV-positive individuals who were enrolled in EAC were included in the study. Additionally, a straightforward random sampling method was used to choose them. Kobo Toolbox was used to collect the data, and STATA version 14.1 was used for analysis. Other variables and the extent of viral load suppression were summarized using descriptive analysis. Utilizing binary logistic regressions, the contributing components were found. Finally, every independent variable in the bivariable analysis with a p-value less than 0.25 was included in the multivariable logistic regression. Eventually, an OR with 95% CI was employed and a p-value of less than 0.05 was deemed statistically significant.
Result
Clients who successfully reduced their viral load were 64.3% of the participants, with a 95% confidence interval of (59.3–68.5). The likelihood of experiencing poor drug adherence (AOR 0.211, 95% CI = 0.117–0.411), being a single client (AOR 0.330, 95% CI = 0.179–0.631), living in a rural area (AOR 0.351, 95% CI = 0.202–0.601), having a history of alcohol consumption (AOR 0.531, 95% CI 0.286–0.985), and chewing khat (AOR 0.223, 95% CI = 0.09–0.545) were less likely to be significantly linked to suppressing viral load.
Conclusion
The target of 70% recommended by the World Health Organization was not achieved in terms of suppressing virus loads. Lower levels of viral load suppression have been associated with variables such alcohol usage, khat chewing, marital status, poor adherence, and site of residence. Thus, it is recommended that ART case managers, adherence counselors, and multidisciplinary teams at medical facilities prioritize clients with low adherence and address behavioral variables in order to optimize viral load suppression.
Economics of Conserving a Tiger Corridor: A Case of Khata Wildlife Corridor in Nepal
Authors: Gurung Bijan , Mendelsohn Robert , Queenborough Simon 
Published: October 2024

Abstract

A consistent concern of international conservation efforts is that relatively poor local communities often bear a high cost for the conservation of wildlife and biodiversity that are highly valued by richer international communities. In this study, we quantify the cost to local farmers from tigers, leopards, and elephants in the Khata wildlife corridor which connects two protected areas adjoining Nepal and India. The losses come from livestock depredation by tiger (Panthera tigris tigris) and its sympatric felid, common leopard (Panthera pardus), as well as loss of rice paddy by elephant (Elephas maximus). We collected data from the records and database of a local natural resource management committee, key informant interviews, and grey and published literature. In the Khata wildlife corridor, the annual livestock loss was estimated to be USD 1,155 for tiger and USD 2,958 for common leopard, and the annual crop loss was USD 2,047 for elephant. Although these sums are relatively small given the international value of these species, they pose a heavy burden for the people living in the corridor. International efforts should help compensate local people for these losses, both to get their cooperation with local conservation and for reasons of equity in distribution of benefits.
The comparative effectiveness of 7 versus 21 home-delivered Medically Tailored Meals to improve malnutrition risk in malnourished patients with heart failure: A random order crossover feeding trial (MEDIMEALS)
Authors: Compher Charlene , Henstenburg Jule Anne , Aloupis Marianne , Sun Amy , Quinn Ryan , Emery Elizabeth , Thomas Jovina , Crafford Adrian , Schwartz Daniel 
Published: October 2024

Abstract

Background: Heart failure (HF) is frequently associated with risk of malnutrition or frank malnutrition. The delivery of medically tailored meals (MTM) to the homes of patients with HF and malnutrition risk or a malnutrition diagnosis after hospital discharge holds promise for improving outcomes.
Methods: A random order crossover study was designed to compare the delivery of 7 versus 21 MTM for four weeks each to patients discharged from the hospital with HF and malnutrition risk. Telephone surveys were conducted at baseline, 30 and 60 days post-discharge to evaluate change in Malnutrition Screening Tool (MST), American Heart Association (AHA) diet goals, sarcopenia risk (SARC-F), and 30-day readmissions.
Results: Forty-six patients were enrolled. Patients had reduced odds of having high MST scores relative to the baseline score at one and two months (OR 0.18, 95% CI 0.04-0.74 and OR 0.21, 95% CI 0.05-0.99, respectively). The AHA diet goal score did not change from baseline at either one or two months. Sarcopenia risk scores improved over time (p=0.01), decreasing by 0.43±0.2 units by one (p=0.03) and 0.59±0.21 units by two months (p=0.007) regardless of the number of MTM provided. Readmissions by 30 days were not significantly different based on the number of MTM provided per week (9% for 21 MTM vs 12.5% for 7 MTM).
Conclusion: The provision of at least seven medically tailored meals per week in the early window after hospital discharge to patients with HF and malnutrition or malnutrition risk is a promising strategy to reduce malnutrition, sarcopenia risk, and improve patient quality of life.