bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis of genetically matched human induced pluripotent stem cells disomic or trisomic for chromosome 21
Authors: Gonzales Patrick , Fonte Gin , Roberts Christine , Jacobsen Connor , Stein Gretchen H. , Link Christopher D. 
Published: January 2017

AbstractTrisomy of chromosome 21, the genetic cause of Down syndrome, has the potential to alter expression of genes on chromosome 21, as well as other locations throughout the genome. These transcriptome changes are likely to underlie the Down syndrome clinical phenotypes. We have employed RNA-seq to undertake an in-depth analysis of transcriptome changes resulting from trisomy of chromosome 21, using induced pluripotent stem cells (iPSCs) derived from a single individual with Down syndrome. These cells were originally derived by Li et al, who genetically targeted chromosome 21 in trisomic iPSCs, allowing selection of disomic sibling iPSC clones. Analyses were conducted on trisomic/disomic cell pairs maintained as iPSCs or differentiated into cortical neuronal cultures. In addition to characterization of gene expression levels, we have also investigated patterns of RNA adenosine-to-inosine editing, alternative splicing, and repetitive element expression, aspects of the transcriptome that have not been significantly characterized in the context of Down syndrome. We identified significant changes in transcript accumulation associated with chromosome 21 trisomy, as well as changes in alternative splicing and repetitive element transcripts. Unexpectedly, the trisomic iPSCs we characterized expressed higher levels of neuronal transcripts than control disomic iPSCs, and readily differentiated into cortical neurons, in contrast to another reported study. Comparison of our transcriptome data with similar studies of trisomic iPSCs suggests that trisomy of chromosome 21 may not intrinsically limit neuronal differentiation, but instead may interfere with the maintenance of pluripotency.
Signs of host genetic regulation in the microbiome composition in cattle
Authors: Gonzalez-Recio O. , Zubiria I. , García-Rodríguez A. , Hurtado A. , Atxaerandio R. 
Published: January 2017

ABSTRACTPrevious studies have revealed certain genetic control by the host over the microbiome composition, although in many species the host genetic link controlling microbial composition is yet unknown. This potential association is important in livestock to study all factors and interactions that rule the effect of the microbiome in complex traits. This report aims to study whether the host genotype exerts any genetic control on the microbiome composition of the rumen in cattle. Data on 16S and 18S rRNA gene-based analysis of the rumen microbiome in 18 dairy cows from two different breeds (Holstein and Brown Swiss) were used. The effect of the genetic background of the animal (through the breed and Single Nucleotide Polymorphisms; SNP) on the relative abundance (RA) of archaea, bacteria and ciliates (with average relative abundance per breed >0.1%) was analysed using Bayesian statistics. In total, 13 genera were analysed for bacteria (5), archaea (1), and ciliates (7). All these bacteria and archaea genera showed association to the host genetic background both for breed and SNP markers, except RA for the generaButyrivibrioandRuminococcusthat showed association with the SNP markers but not with the breed composition. Relative abundance of 57% (4/7) of ciliate analysed showed to be associated to the genetic background of the host. This host genetic link was observed in some genus ofTrichostomatiafamily. For instance, the breed had a significant effect onIsotricha,OphryoscolexandPolyplastron, and the SNP markers onEntodinium,OphryoscolexandPolyplastron. In total, 77% (10/13) of microbes analysed showed to be associated to the host genetic background (either by breed or SNP genotypes). Further, the results showed a significant association between DGAT1, ACSF3, AGPAT3 and STC2 genes with the relative abundancePrevotellagenus with a false discovery rate lower than 15%. The results in this study support the hypothesis and provide some evidence that there exist a host genetic component in cattle that can partially regulate the composition of the microbiome.
MCF-7 breast cancer cell line PacBio generated transcriptome has ~300 novel transcribed regions, un-annotated in both RefSeq and GENCODE, and absent in the liver, heart and brain transcriptomes
Authors: Chakraborty Sandeep 
Published: January 2017

AbstractIlluminating the ‘dark’ regions of the human genome remains an ongoing effort, a decade and a half after the human genome was sequenced - RefSeq and GENCODE being two of the major annotation databases. Pacific Biosciences (PacBio) has provided open access to the transcriptome of MCF-7, a breast cancer cell line that has provided significant therapeutic advancement in breast cancer research since the 1970s. PacBio sequencing generates much longer reads compared to second-generation sequencing technologies, with a trade-off of lower throughput, higher error rate and more cost per base. Here, this transcriptome was analyzed using the YeATS pipeline, with additionally introduced kmer based algorithms, reducing computational times to a few hours on a simple workstation. Out of ~300 transcripts that have no match in both RefSeq and GENCODE, ~250 are absent in the transcriptomes of the heart, liver and brain, also provided by PacBio. Also, ~200 transcripts are absent in a recent catalogue of un-annotated long non-coding RNAs from 6,503 samples (~43 Terabases of sequence data) [1], and among 2,556 novel transcripts reported in an experimental workflow RACE-Seq [2]. 65 transcripts have >100 amino acid open reading frames, and have the potential of being protein coding genes. ORF based annotation also identified few bacterial transcripts in the PacBio database mapped to the human genome, and one human transcript that has been annotated as bacterial in the NCBI database. The current work reiterates the under-utilization of transcriptomes for annotating genomes. It also provides new leads for investigating breast cancer by virtue of exclusively expressed transcripts not expressed in other tissues, which have the prospects of breast cancer biomarkers based on further investigations.
Thinking style across cultures: an interview with Richard Nisbett
Authors: Lee Hyun-Jung 
Published: January 2017

Purpose
The purpose of this paper is to gain some insights from a leading scholar of the cross-cultural cognitive social psychology field on how cultural differences are viewed, understood, and dealt with, and thus to contribute to enrich the way cultural differences are framed in cross-cultural management research.

Design/methodology/approach
The author conducts a formal, semi-structured interview with Richard Nisbett for a duration of 90 minutes. The author extracts the key message from the interview and re-structures the conversation in a meaningful manner.

Findings
From his cognitive social psychology lens, Richard Nisbett views that any cross-cultural contact between different thinking styles is advantageous because differences help address the limitations of one’s own thinking style.

Research limitations/implications
The insights from cross-cultural cognitive social psychology encourage cross-cultural management researchers to further investigate the positive consequences of cultural differences.

Originality/value
Richard Nisbett’s own journey from a young scientist who describes himself as an extreme universalist, to a mature intellectual who understands and appreciates different thinking style, is itself a concrete example of how differences can lead to the positive. The author summarizes three factors that are key to a positive outcome of cultural differences: curiosity and openness to cultural differences; habit of critical thinking; and intense interaction with culturally different others.
The upside of cultural differences
Authors: Stahl Günter K. , Miska Christof , Lee Hyun-Jung , De Luque Mary Sully 
Published: January 2017

PurposeThe purpose of this paper is to encourage scholars to look at commonly considered phenomena in international business and cross-cultural research in new ways and to theorize and explore how cultural diversity, distance, and foreignness create value for global organizations. These considerations should result in a more balanced treatment of culture in cross-cultural management (CCM) research.Design/methodology/approachThe idea that there are negative consequences associated with cultural differences is pervasive in hypotheses formulation and empirical testing in international business and CCM literature, as reflected in widely used constructs such as “cultural distance,” “cultural misfit,” “foreignness,” and related concepts. Consistent with a Positive Organizational Scholarship (POS) perspective on culture and cultural differences, the authors emphasize the positive role of distance and diversity across national, cultural, institutional, and organizational dimensions. In addition, they provide an overview of the contributions to the special issue.FindingsExamining the positive side of culture is not only beneficial theoretically in terms of filling the existing gaps in the literature, but is also crucial for the practice of international and global business. Accordingly, the contributions to the special issue highlight how explicitly considering positive phenomena can help better understand when and how cultural diversity, distance, and foreignness can enhance organizational effectiveness and performance at multiple levels. They include five research papers, a Distinguished Scholar Essay by Kim Cameron, the Founder of the POS movement, and an interview piece with Richard Nisbett, a Pioneer Researcher in culture and cognition.Originality/valueThe overemphasis on adverse outcomes associated with cultural differences in existing research has hindered the understanding of the processes and conditions that help organizations leverage the benefits of cultural differences in a wide range of contexts. This introductory paper together with the contributions included in the special issue showcases the positive dynamics and outcomes associated with cultural differences, distance, and diversity in a wide range of international business contexts.
Effects of woven liners treated by LaCl3 or CeO2 solution on film formation mechanisms of self-lubricating radial spherical plain bearings
Authors: Qiu Ming , Miao Yanwei , Li Yingchun , Chen Long , Hu Rensong , Lu Jianjun 
Published: January 2017

Purpose
The fabric self-lubricating liners are the key factors impacting the performances of self-lubricating spherical plain bearings. The purpose of this paper is to improve the friction and wear properties of self-lubricating radial spherical plain bearings by modification of the liners.

Design/methodology/approach
The liners of hybrid woven PTFE/Kevlar fabrics were treated respectively by the LaCl3 and CeO2 solutions. The tribological properties of self-lubricating spherical plain bearings with treated or untreated liners under continuous swaying conditions were investigated with the bearing tester at the swaying frequency of 2.5 Hz and the swaying angle of ±10°. The film formation and wear mechanisms were analyzed based on the observation of worn surfaces with a scanning electron microscope (SEM) and an energy dispersive spectrometer (EDS).

Findings
Results show that the tribological properties of the bearings treated by the LaCl3 or CeO2 solution were improved compared with those of the untreated bearings. In particular, the wear resistance of bearings treated by the CeO2 solution was remarkably improved under higher swaying cycles, but the anti-friction properties and cooling effects of bearings treated by the LaCl3 solution were better under lower swaying cycles. Through SEM analysis, the reasons were analyzed. The bearings with treated liners only produced slight adhesive and abrasive wear, but the bearings with untreated liners produced more serious adhesive and abrasive wear under higher swaying cycles.

Originality/value
The paper proposed a new pretreatment process for the self-lubricating liners. The investigation on the friction and wear behaviors of the bearings is beneficial for prolonging the service lives of the radial spherical plain bearings.
A comparative study on simulation and experiment of oil-air lubrication unit for high speed bearing
Authors: Zeng Qunfeng , Zhang Jinhua , Hong Jun , Liu Cheng 
Published: January 2017

Purpose
The purpose of this paper is to design an oil-air lubrication system with low temperature rise, vibration and noise simplifies the spindle configuration. The oil-air lubrication unit is a key component for high-speed grinding machine tools. The development of oil-air lubrication unit suitable for high/ultrahigh rotational speed is a daunting task owing to the lubrication challenges.

Design/methodology/approach
This paper emphasizes three main issues: the analysis of oil-air two-phase flow for tradition oil-air lubrication unit with the simulation method; the design of new oil-air lubrication unit for the high/ultrahigh-speed grinding machine tools and the comparative experiment research of tradition and new oil-air lubrication unit. The optimum structure parameters that create the optimum flow pattern and operating conditions resulting in low temperature increase, vibration and noise of oil-air lubricated spindle can be achieved by the simulation method and experiments.

Findings
The simulation and experimental results show that new oil-air lubrication unit lubricating a high speed electric spindle has a better performance with a small temperature increase and vibration, which means that our proposed method is an effective design method for oil-air lubrication system.

Originality/value
A design method suitable for high-speed oil-air lubrication unit is proposed. New oil-air lubrication unit is expected to apply for high/ultrahigh rotational speed grinding machine tools.
Effect of recess configuration on the performances of high-speed hybrid journal bearing
Authors: Lin Qiyin , Wei Zhengying , Wang Ning , Zhang Yubin 
Published: January 2017

Purpose
The purpose of this paper is to study the influences of recess configurations on the performances of high-speed hybrid journal bearing. Hybrid journal bearing earns increasing attention in high-speed machine tool spindle owing to its intrinsic outstanding performances of low temperature rise and high stability.

Design/methodology/approach
To investigate the coupled effects of temperature, turbulence and the interaction between lubricant and journal/bearing bush, a thermal fluid-structure interaction approach is presented and validated by the experimental results.

Findings
Ladder-type recess has excellent tribological characteristics in decreasing temperature rise, improving stability and inhibiting cavitation, which are all beneficial to improve the performances of high-speed spindle system.

Originality/value
This work can be a valuable guide for the future high-speed hybrid journal bearing design.
Investigations on transitions of lubrication states for water lubricated bearing. Part II: further insight into the film thickness ratio lambda
Authors: Xie Zhongliang , Rao Zhu-shi , Ta Na , Liu Ling 
Published: January 2017

Purpose
As the companion paper of Part I, this paper aims to get more insight into the essence of lambda and to reveal its nature and role in the transition of lubrication states. Mixed lubrication (ML) model with micro-asperities contacts has been discussed in details in Part I.

Design/methodology/approach
Mimetic algorithm is used to get numerical solutions. Relationships between film thickness ratios and lubrication states transition with different external loads, rotating speeds, radial clearances, elastic modulus, surface hardness and roughness parameters are obtained.

Findings
The characteristic parameters of transitions from boundary lubrication (BL) to ML and ML to hydrodynamic lubrication (HL) are studied to determine how these parameters change with above factors. Finally, the essence and major influencing factors of lambda are summarized for such bearings.

Originality/value
In Part II, the authors believe that the paper presents for the first time: further insight into the essence of the lambda ratio, and its role in the lubrication states transition are given; the determinations of the characteristic parameters of transition from BL to ML and ML to HL are investigated for the first time; the characteristic parameters of transitions from BL to ML and ML to HL are also studied to determine how parameters (external load, rotating speed, radial clearance, elastic modulus, surface hardness and roughness parameter) change with above factors; a summary of the essence and major influencing factors of lambda for such bearings is given.
Antecedents of word of mouth behaviour among female grocery shoppers in Iran
Authors: Harris Patricia , Khatami Nasim Alsadat 
Published: January 2017

Purpose
The purpose of this paper is to report on research on the antecedents of word of mouth (WOM) behaviour among Iranian women in the context of grocery shopping.

Design/methodology/approach
Using a survey instrument, the authors empirically test the conceptual model of De Matos and Rossi.

Findings
This paper finds support for the model and finds satisfaction, commitment, loyalty, trust, perceived value and quality to be significant antecedents of WOM. Commitment is found to be the most significant predictor of WOM.

Research limitations/implications
This paper examines only factors affecting the initiation of positive WOM; future research should examine also drivers of negative WOM. The study sample consists of female shoppers at Tehran branches of one grocery retailer. There is scope to extend the research to shoppers of other grocery retailers and to other parts of Iran.

Practical implications
The findings provide useful insight for practitioners in identifying the relative importance of drivers of WOM. This research suggests that satisfaction is the weakest predictor of WOM, and therefore, it is dangerous for grocery retail managers to rely on customer satisfaction metrics as indicators of WOM initiation.

Originality/value
This research appears to be the first study of WOM in the context of Iranian grocery retailing, a sector which is both fast growing and undergoing structural change.
Effect of religiosity on luxury consumer behavior: the case of the Tunisian Muslim
Authors: Dekhil Fawzi , Boulebech Hajer , Bouslama Neji 
Published: January 2017

Purpose
The purpose of this paper is to describe the effects of religiosity on attitude and personal orientation toward luxury brands and on purchase and repurchase intentions. Determining the effects of religiosity on the consumers’ behavior toward luxury has proved to be a crucial matter. As far as the authors know, academic research on this topic is almost non-existent. This is an exploratory study at the level of the direct effects of religiosity. Following a literature review, a model was constructed to represent the various interrelationships between the variables investigated in this research.

Design/methodology/approach
A quantitative study was conducted among 227 individuals having varying levels of religiosity. The authors verified, before, that those interviewed have purchased a luxury brand.

Findings
The findings show that religiosity does not hinder the consumption of luxury brands. Indeed, the authors detected a positive relationship between attitude and personal orientation toward luxury brands and the level of religiosity. The effect of religiosity on attitude and personal orientation is stronger for persons having higher incomes. Moreover, this effect is slight more marked among women.

Research limitations/implications
Just as for all other research work, it is important to identify the limitations of this study. The authors need only to acknowledge its exploratory nature for these relationships to be identified as preliminary ones and as the first elements of proof rather than as a conclusive demonstration. This research suffers from certain other limitations, especially concerning its convenience sampling and the fact that it covered only a limited geographical area, namely, the capital, Tunis, and its adjacent suburbs.

Practical implications
This research has shown that whatever the level of religiosity, its effect is stronger among persons having a high income. Therefore, Muslims, whatever their religiosity, can be a target for marketers and luxury brand.

Social implications
Islam is not against luxury brand but against ostentation.

Originality/value
It is the first research, as the authors know, that study the relationship between religiosity and consumer luxury behavior, especially among Muslims.
Boycotting foreign products: a study of Indonesian Muslim consumers
Authors: Sari Dessy Kurnia , Mizerski Dick , Liu Fang 
Published: January 2017

Purpose
This paper aims to investigate the motivations behind Muslim consumers’ boycotting of foreign products. The act of boycotting foreign products has become increasingly common among Muslim consumers. Products from different countries-of-origin are their boycott targets.

Design/methodology/approach
The study adopted semi-structured in-depth interviews and focus-group discussions for data collection. A total of 36 Indonesian subjects participated in the study, representing the “university student” and “non-university student” samples. Leximancer, a qualitative analytical tool, was used to identify important motivations for boycotting behaviour among Muslim consumers.

Findings
Contrary to previous findings, this study found that Muslim consumers do not boycott solely for religious reasons. For example, most participants reported they boycotted Chinese products because they would like to protect their local products, along with the religious-based motivation of rejecting uncertainty about the halal certification of the products. Thus, the motivations identified from this study were not related exclusively to religion.

Practical implications
The present study offers new insights into the religious and secular motivations of Muslim consumers’ boycotts. Foreign products should adopt localised strategies such as repeatedly reminding consumers of the true halal nature of their products and their contribution to the local people.

Originality/value
This study contributes to the recognition of new insights into Muslim motivation to boycott product. The results develop important concepts surrounding the issue of boycotting foreign products. A concept map has been produced to offer a more comprehensive picture of Muslim’s boycotting behaviour.