bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of beam splitter in a zero-refractive-index photonic crystal at the frequency of Dirac-like point
Authors: Qiu Pingping , Qiu Weibin , Lin Zhili , Chen Houbo , Ren Junbo , Wang Jia-Xian , Kan Qiang , Pan Jiao-Qing 
Published: August 2017

AbstractThe Dirac-like cone dispersion of the photonic crystal induced by the three-fold accidental degeneracy at the Brillouin center is calculated in this paper. Such photonic crystals can be mapped to zero-refractive-index materials at the vicinity of the Dirac-like point frequency, and utilized to construct beam splitter of high transmission efficiency. The splitting ratio is studied as a function of the position of the input/output waveguides. Furthermore, variant beam splitters with asymmetric structures, bulk defects, and some certain bending angles are numerically simulated. Finally, we show that 1 × 2 to 1 × N beam splitting can be realized with high transmission efficiency in such a zero-refractive-index photonic crystal at the frequency of Dirac-like point. The proposed structure could be a fundamental component of the high density photonic integrated circuit technique.
Connecting individual to collective cell migration
Authors: George Mishel , Bullo Francesco , Campàs Otger 
Published: August 2017

AbstractCollective cell migration plays a pivotal role in the formation of organs, tissue regeneration, wound healing and many disease processes, including cancer. Despite the considerable existing knowledge on the molecular control of cell movements, it is unclear how the different observed modes of collective migration, especially for small groups of cells, emerge from the known behaviors of individual cells. Here we derive a physical description of collective cellular movements from first principles, while accounting for known phenomenological cell behaviors, such as contact inhibition of locomotion and force-induced cell repolarization. We show that this theoretical description successfully describes the motion of groups of cells of arbitrary numbers, connecting single cell behaviors and parameters (e.g., adhesion and traction forces) to the collective migration of small groups of cells and the expansion of large cell colonies. Specifically, using a common framework, we explain how cells characterized by contact inhibition of locomotion can display coherent collective behavior when in groups, even in the absence of biochemical signaling. We find an optimal group size leading to maximal group persistence and show that cell proliferation prevents the buildup of intercellular forces within cell colonies, enabling their expansion.
Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3
Authors: Xie Chunmei , Tiede Christian , Zhang Xuanyi , Wang Congrong , Li Zhixiong , Xu Xiao , McPherson Michael J. , Tomlinson Darren C. , Xu Weiwen 
Published: August 2017

AbstractGlypican-3 (GPC3) is a promising new marker for hepatocellular carcinoma, but the reported values for serum GPC3 differ markedly between currently available kits. Here we isolated Affimer non-antibody binding proteins against GPC3 by phage display and developed a new sandwich chemiluminescence immunoassay (CLIA) combining an Affimer with a monoclonal antibody (Affimer-MAb CLIA). The proposed CLIA assay demonstrated a wide linear range  0.03–600 ng/mL) with a good linear correlation coefficient (0.9999), a high detection limitation (0.03 ng/mL) and specificity (0–0.002%) for detection of GPC3. The accuracy, hook effect and stability were demonstrated to be satisfactory. The mean level of GPC3 in serum was higher (>8.5 fold, P < 0.001) in hepatocellular carcinoma patients compared to healthy and other liver disease individuals. A poor correlation (correlation coefficients ranged from −0.286 to 0.478) was observed through pairwise comparison within different kits. However, only this newly developed CLIA test showed high specificity and correlated with the “gold standard” GPC3-immunohistochemistry. This study indicates that Affimer-MAb CLIA can be used to generate a sensitive immunodiagnostic kit, which offers the potential for a highly specific clinically-relevant detection system.
External validation of the NUn score for predicting anastomotic leakage after oesophageal resection
Authors: Paireder Matthias , Jomrich Gerd , Asari Reza , Kristo Ivan , Gleiss Andreas , Preusser Matthias , Schoppmann Sebastian F. 
Published: August 2017

AbstractEarly detection of anastomotic leakage (AL) after oesophageal resection for malignancy is crucial. This retrospective study validates a risk score, predicting AL, which includes C-reactive protein, albumin and white cell count in patients undergoing oesophageal resection between 2003 and 2014. For validation of the NUn score a receiver operating characteristic (ROC) curve is estimated. Area under the ROC curve (AUC) is reported with 95% confidence interval (CI). Among 258 patients (79.5% male) 32 patients showed signs of anastomotic leakage (12.4%). NUn score in our data has a median of 9.3 (range 6.2–17.6). The odds ratio for AL was 1.31 (CI 1.03–1.67; p = 0.028). AUC for AL was 0.59 (CI 0.47–0.72). Using the original cutoff value of 10, the sensitivity was 45.2% an the specificity was 73.8%. This results in a positive predictive value of 19.4% and a negative predictive value of 90.6%. The proportion of variation in AL occurrence, which is explained by the NUn score, was 2.5% (PEV = 0.025). This study provides evidence for an external validation of a simple risk score for AL after oesophageal resection. In this cohort, the NUn score is not useful due to its poor discrimination.
Association between selected antimicrobial resistance genes and antimicrobial exposure in Danish pig farms
Authors: Birkegård Anna Camilla , Halasa Tariq , Græsbøll Kaare , Clasen Julie , Folkesson Anders , Toft Nils 
Published: August 2017

AbstractBacterial antimicrobial resistance (AMR) in pigs is an important public health concern due to its possible transfer to humans. We aimed at quantifying the relationship between the lifetime exposure of antimicrobials and seven antimicrobial resistance genes in Danish slaughter pig farms. AMR gene levels were quantified by qPCR of total-community DNA in faecal samples obtained from 681 batches of slaughter pigs. The lifetime exposure to antimicrobials was estimated at batch level for the piglet, weaner, and finisher periods individually for the sampled batches. We showed that the effect of antimicrobial exposure on the levels of AMR genes was complex and unique for each individual gene. Several antimicrobial classes had both negative and positive correlations with the AMR genes. From 10–42% of the variation in AMR gene levels could be explained in the final regression models, indicating that antimicrobial exposure is not the only important determinant of the AMR gene levels.
TCA cycle rewiring fosters metabolic adaptation to oxygen restriction in skeletal muscle from rodents and humans
Authors: Capitanio Daniele , Fania Chiara , Torretta Enrica , Viganò Agnese , Moriggi Manuela , Bravatà Valentina , Caretti Anna , Levett Denny Z. H. , Grocott Michael P. W. , Samaja Michele , Cerretelli Paolo , Gelfi Cecilia 
Published: August 2017

AbstractIn mammals, hypoxic stress management is under the control of the Hypoxia Inducible Factors, whose activity depends on the stabilization of their labile α subunit. In particular, the skeletal muscle appears to be able to react to changes in substrates and O2 delivery by tuning its metabolism. The present study provides a comprehensive overview of skeletal muscle metabolic adaptation to hypoxia in mice and in human subjects exposed for 7/9 and 19 days to high altitude levels. The investigation was carried out combining proteomics, qRT-PCR mRNA transcripts analysis, and enzyme activities assessment in rodents, and protein detection by antigen antibody reactions in humans and rodents. Results indicate that the skeletal muscle react to a decreased O2 delivery by rewiring the TCA cycle. The first TCA rewiring occurs in mice in 2-day hypoxia and is mediated by cytosolic malate whereas in 10-day hypoxia the rewiring is mediated by Idh1 and Fasn, supported by glutamine and HIF-2α increments. The combination of these specific anaplerotic steps can support energy demand despite HIFs degradation. These results were confirmed in human subjects, demonstrating that the TCA double rewiring represents an essential factor for the maintenance of muscle homeostasis during adaptation to hypoxia.
The MC4R genotype is associated with postpartum weight reduction and glycemic changes among women with prior gestational diabetes: longitudinal analysis
Authors: de Carvalho Aline Martins , Shao Ping , Liu Huikun , Cheng Han-Ling , Zheng Yan , Leng Junhong , Li Weiqin , Huang Tao , Wang Tiange , Wang Leishen , Zhang Shuang , Hu Gang , Qi Lu 
Published: August 2017

AbstractThe genetic variants near the Melanocortin-4 receptor gene (MC4R), a key protein regulating energy balance and adiposity, have been related to obesity and glucose metabolism. We aimed to assess whether the MC4R genotype affected longitudinal changes in body weight and glucose metabolism biomarkers among women with prior gestational diabetes mellitus (GDM). The MC4R genotype, postpartum weight reduction, and glycemic changes between after delivery and pregnancy were assessed in a cohort of 1208 Chinese women who had experienced GDM. The adiposity-increasing allele (C) of the MC4R variant rs6567160 was associated with greater postpartum increase of HbA1c (β = 0.08%; P = 0.03) and 2-hour OGTT glucose concentrations (β = 0.25 mmol/L; P = 0.02). In addition, we found an interaction between the MC4R genotype and postpartum weight reduction on changes in fasting plasma glucose (P-interaction = 0.03). We found that the MC4R genotype was associated with postpartum glycemic changes; and the association with fasting glucose were significantly modified by postpartum weight reduction in women who had experienced GDM.
Prognosis of HIV Patients Receiving Antiretroviral Therapy According to CD4 Counts: A Long-term Follow-up study in Yunnan, China
Authors: Ren Li , Li Juan , Zhou Shiyi , Xia Xueshan , Xie Zhenrong , Liu Pan , Xu Yu , Qian Yuan , Zhang Huifeng , Ma Litang , Pan Qiuwei , Wang Kunhua 
Published: August 2017

AbstractWe aim to evaluate the overall survival and associated risk factors for HIV-infected Chinese patients on antiretroviral therapy (ART). 2517 patients receiving ART between 2006 and 2016 were prospectively enrolled in Yunnan province. Kaplan-Meier analyses and Cox proportional hazard regression analyses were performed. 216/2517 patients died during a median 17.5 (interquartile range [IQR] 6.8–33.2) months of follow-up. 82/216 occurred within 6 months of starting ART. Adjusted hazard ratios were10.69 (95%CI 2.38–48.02, p = 0.002) for old age, 1.94 (95%CI 1.40–2.69, p < 0.0001) for advanced WHO stage, and 0.42 (95%CI 0.27–0.63, p < 0.0001) for heterosexual transmission compared to injecting drug users. Surprisingly, adjusted hazard ratios comparing low CD4 counts group (<50 cells/µl) with high CD4 counts group (≥500 cells/µl) within six months after starting ART was 20.17 (95%CI 4.62–87.95, p < 0.0001) and it declined to 3.57 (95%CI 1.10–11.58, p = 0.034) afterwards. Age, WHO stage, transmission route are significantly independent risk factors for ART treated HIV patients. Importantly, baseline CD4 counts is strongly inversely associated with survival in the first six months; whereas it becomes a weak prognostic factor after six months of starting ART.
No effects of transcranial DLPFC stimulation on implicit task sequence learning and consolidation
Authors: Savic Branislav , Cazzoli Dario , Müri René , Meier Beat 
Published: August 2017

AbstractNeurostimulation of the dorsolateral prefrontal cortex (DLPFC) can modulate performance in cognitive tasks. In a recent study, however, transcranial direct current stimulation (tDCS) of the DLPFC did not affect implicit task sequence learning and consolidation in a paradigm that involved bimanual responses. Because bimanual performance increases the coupling between homologous cortical areas of the hemispheres and left and right DLPFC were stimulated separately the null findings may have been due to the bimanual setup. The aim of the present study was to test the effect of neuro-stimulation on sequence learning in a uni-manual setup. For this purpose two experiments were conducted. In Experiment 1, the DLPFC was stimulated with tDCS. In Experiment 2 the DLPFC was stimulated with transcranial magnetic stimulation (TMS). In both experiments, consolidation was measured 24 hours later. The results showed that sequence learning was present in all conditions and sessions, but it was not influenced by stimulation. Likewise, consolidation of sequence learning was robust across sessions, but it was not influenced by stimulation. These results replicate and extend previous findings. They indicate that established tDCS and TMS protocols on the DLPFC do not influence implicit task sequence learning and consolidation.
Sex-specific eNOS activity and function in human endothelial cells
Authors: Cattaneo Maria Grazia , Vanetti Claudia , Decimo Ilaria , Di Chio Marzia , Martano Giuseppe , Garrone Giulia , Bifari Francesco , Vicentini Lucia Maria 
Published: August 2017

AbstractClinical and epidemiological data show that biological sex is one of the major determinants for the development and progression of cardiovascular disease (CVD). Impaired endothelial function, characterized by an imbalance in endothelial Nitric Oxide Synthase (eNOS) activity, precedes and accelerates the development of CVD. However, whether there is any sexual dimorphism in eNOS activity and function in endothelial cells (ECs) is still unknown. Here, by independently studying human male and female ECs, we found that female ECs expressed higher eNOS mRNA and protein levels both in vitro and ex vivo. The increased eNOS expression was associated to higher enzymatic activity and nitric oxide production. Pharmacological and genetic inhibition of eNOS affected migratory properties only in female ECs. In vitro angiogenesis experiments confirmed that sprouting mostly relied on eNOS-dependent migration in female ECs. At variance, capillary outgrowth from male ECs was independent of eNOS activity but required cell proliferation. In this study, we found sex-specific differences in the EC expression, activity, and function of eNOS. This intrinsic sexual dimorphism of ECs should be further evaluated to achieve more effective and precise strategies for the prevention and therapy of diseases associated to an impaired endothelial function such as CVD and pathological angiogenesis.
Printable Fluorescent Hydrogels Based on Self-Assembling Peptides
Authors: Xia Yifan , Xue Bin , Qin Meng , Cao Yi , Li Ying , Wang Wei 
Published: August 2017

AbstractFluorescent hydrogels (FH) have a variety of potential applications in the field of soft electronics. However, fabrication of mechanically stable and printable fluorescent hydrogels remains challenging. Here, we report a kind of fluorescent hydrogel based on the co-assembly of peptide motif and transition metal ions. The metal ions are captured in the hydrogel network at specific positions through covalently linked ligands on the peptide hydrogelators. This efficiently prevents the aggregation and self-quenching of organometallic chromophores. In addition, the formation of metal-ligand complexes introduces additional interactions to stabilize the hydrogel network, making the FH even more stable after the incorporation of metal ions. The FH is optically transparent but highly fluorescent. By using three different metal ions, the white light fluorescent supramolecular hydrogel has been achieved. As a proof-of-principle, we demonstrate the printability of the hydrogels to various patterns. We anticipate that with the improved fluorescent performance and stability, this kind of FH can find broad applications in extrusion-based 3D printing for the construction of soft electronics.
Radio frequency surface plasma oscillations: electrical excitation and detection by Ar/Ag(111)
Authors: Serrano Giulia , Tebi Stefano , Wiespointner-Baumgarthuber Stefan , Müllegger Stefan , Koch Reinhold 
Published: August 2017

AbstractWe electrically excite surface plasma oscillations on a Ag(111) single crystal by alternating electric charging at radio frequency. The radio frequency signal energy of 2.2 μeV, used to induce surface plasma oscillations, is about 5 to 6 orders of magnitude lower than the plasmon energies reachable by optical excitation or electron impact. The detection of the surface plasma oscillations is achieved by nano-fabricated 2D single-crystal sensor-islands of Ar atoms, which are shown by imaging with a scanning tunneling microscope to restructure in response to the radio frequency surface plasma oscillations, providing nanometer spatial resolution and a characteristic decay time of ≈150 ns.
Reproductive benefits of no-take marine reserves vary with region for an exploited coral reef fish
Authors: Carter A. B. , Davies C. R. , Emslie M. J. , Mapstone B. D. , Russ G. R. , Tobin A. J. , Williams A. J. 
Published: August 2017

AbstractNo-take marine reserves (NTMRs) are expected to benefit fisheries via the net export of eggs and larvae (recruitment subsidy) from reserves to adjacent fished areas. Quantifying egg production is the first step in evaluating recruitment subsidy potential. We calculated annual egg production per unit area (EPUA) from 2004 to 2013 for the commercially important common coral trout, Plectropomus leopardus, on fished and NTMR reefs throughout the Great Barrier Reef (GBR), Australia. Geographic region, NTMR status, fish size, and population density were all found to affect EPUA. The interactions among these factors were such that, EPUA on NTMR reefs compared to reefs open to fishing was 21% greater in the southern GBR, 152% greater in the central GBR, but 56% less in the northern GBR. The results show that while NTMRs can potentially provide a substantial recruitment subsidy (central GBR reefs), they may provide a far smaller subsidy (southern GBR), or serve as recruitment sinks (northern GBR) for the same species in nearby locations where demographic rates differ. This study highlights the importance of considering spatial variation in EPUA when assessing locations of NTMRs if recruitment subsidy is expected from them.
Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
Authors: Liu G. Y. , Agarwal R. , Ko K. R , Ruthven M. , Sarhan H. T. , Frampton J. P. 
Published: August 2017

AbstractCollagen is widely used in tissue engineering and regenerative medicine, with many examples of collagen-based biomaterials emerging in recent years. While there are numerous methods available for forming collagen scaffolds from isolated collagen, existing biomaterial processing techniques are unable to efficiently align collagen at the microstructural level, which is important for providing appropriate cell recognition and mechanical properties. Although some attention has shifted to development of fiber-based collagen biomaterials, existing techniques for producing and aligning collagen fibers are not appropriate for large-scale fiber manufacturing. Here, we report a novel biomaterial fabrication approach capable of efficiently generating collagen fibers of appropriate sizes using a viscous solution of dextran as a dissolvable template. We demonstrate that myoblasts readily attach and align along 2D collagen fiber networks created by this process. Furthermore, encapsulation of collagen fibers with myoblasts into non-cell-adherent hydrogels promotes aligned growth of cells and supports their differentiation. The ease-of-production and versatility of this technique will support future development of advanced in vitro tissue models and materials for regenerative medicine.
Localization and Orientation of Xanthophylls in a Lipid Bilayer
Authors: Grudzinski Wojciech , Nierzwicki Lukasz , Welc Renata , Reszczynska Emilia , Luchowski Rafal , Czub Jacek , Gruszecki Wieslaw I. 
Published: August 2017

AbstractXanthophylls (polar carotenoids) play diverse biological roles, among which are modulation of the physical properties of lipid membranes and protection of biomembranes against oxidative damage. Molecular mechanisms underlying these functions are intimately related to the localization and orientation of xanthophyll molecules in lipid membranes. In the present work, we address the problem of localization and orientation of two xanthophylls present in the photosynthetic apparatus of plants and in the retina of the human eye, zeaxanthin and lutein, in a single lipid bilayer membrane formed with dimyristoylphosphatidylcholine. By using fluorescence microscopic analysis and Raman imaging of giant unilamellar vesicles, as well as molecular dynamics simulations, we show that lutein and zeaxanthin adopt a very similar transmembrane orientation within a lipid membrane. In experimental and computational approach, the average tilt angle of xanthophylls relative to the membrane normal is independently found to be ~40 deg, and results from hydrophobic mismatch between the membrane thickness and the distance between the terminal hydroxyl groups of the xanthophylls. Consequences of such a localization and orientation for biological activity of xanthophylls are discussed.
Perturbation of formate pathway and NADH pathway acting on the biohydrogen production
Authors: Liu Dong , Sun Yunze , Li Yuhao , Lu Yuan 
Published: August 2017

AbstractThe formate pathway and NADH pathway as two common hydrogen-producing metabolic pathways have been well characterized to understand and improve biohydrogen production. These two pathways have been thought to be separate and have been independently investigated. However, in this study, perturbation of genes (hycA,fdhF,fhlA,ldhA,nuoB,hybO,fdh1,narP, andppk) inEnterobacter aerogenesrelated to the formate pathway or NADH pathway revealed that these two pathways affected each other. Further metabolic analysis suggested that a linear relationship existed between the relative change of hydrogen yield in the formate pathway or NADH pathway and the relative change of NADH yield or ATP yield. Thus, this finding provides new insight into the role of cellular reducing power and energy level in the hydrogen metabolism. It also establishes a rationale for improving hydrogen production from a global perspective.
Key glycolytic branch influences mesocarp oil content in oil palm
Authors: Ruzlan Nurliyana , Low Yoke Sum Jaime , Win Wilonita , Azizah Musa Noor , Ong Ai-Ling , Chew Fook-Tim , Appleton David , Mohd Yusof Hirzun , Kulaveerasingam Harikrishna 
Published: August 2017

AbstractThe fructose-1,6-bisphosphate aldolase catalyzed glycolysis branch that forms dihydroxyacetone phosphate and glyceraldehyde-3-phosphate was identified as a key driver of increased oil synthesis in oil palm and was validated in Saccharomyces cerevisiae. Reduction in triose phosphate isomerase (TPI) activity in a yeast knockdown mutant resulted in 19% increase in lipid content, while yeast strains overexpressing oil palm fructose-1,6-bisphosphate aldolase (EgFBA) and glycerol-3-phosphate dehydrogenase (EgG3PDH) showed increased lipid content by 16% and 21%, respectively. Genetic association analysis on oil palm SNPs of EgTPI SD_SNP_000035801 and EgGAPDH SD_SNP_000041011 showed that palms harboring homozygous GG in EgTPI and heterozygous AG in EgGAPDH exhibited higher mesocarp oil content based on dry weight. In addition, AG genotype of the SNP of EgG3PDH SD_SNP_000008411 was associated with higher mean mesocarp oil content, whereas GG genotype of the EgFBA SNP SD_SNP_000007765 was favourable. Additive effects were observed with a combination of favourable alleles in TPI and FBA in Nigerian x AVROS population (family F7) with highest allele frequency GG.GG being associated with a mean increase of 3.77% (p value = 2.3E−16) oil content over the Family 1. An analogous effect was observed in yeast, where overexpressed EgFBA in TPI
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resulted in a 30% oil increment. These results provide insights into flux balances in glycolysis leading to higher yield in mesocarp oil-producing fruit.