bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring the diffusion behavior of Na,K-ATPase by fluorescence correlation spectroscopy (FCS) upon fluorescence labelling with eGFP or Dreiklang
Authors: Junghans Cornelia , Schmitt Franz-Josef , Vukojević Vladana , Friedrich Thomas 
Published: March 2016

AbstractMeasurement of lateral mobility of membraneembedded proteins in living cells with high spatial and temporal precision is a challenging task of optofluidics. Biological membranes are complex structures, whose physico-chemical properties depend on the local lipid composition, cholesterol content and the presence of integral or peripheral membrane proteins, which may be involved in supramolecular complexes or are linked to cellular matrix proteins or the cytoskeleton. The high proteinto- lipid ratios in biomembranes indicate that membrane proteins are particularly subject to molecular crowding, making it difficult to follow the track of individual molecules carrying a fluorescence label. Novel switchable fluorescence proteins such as Dreiklang [1], are, in principle, promising tools to study the diffusion behavior of individual molecules in situations of molecular crowding due to excellent spectral control of the ON- and OFF-switching process. In this work, we expressed an integral membrane transport protein, the Na,K-ATPase comprising the human α2-subunit carrying an N-terminal eGFP or Dreiklang tag and human β1-subunit, in HEK293T cells and measured autocorrelation curves by fluorescence correlation spectroscopy (FCS). Furthermore,we measured diffusion times and diffusion constants of eGFP and Dreiklang by FCS, first, in aqueous solution after purification of the proteins upon expression in E. coli, and, second, upon expression as soluble proteins in the cytoplasm of HEK293T cells. Our data show that the diffusion behavior of the purified eGFP and Dreiklang in solution as well as the properties of the proteins expressed in the cytoplasm are very similar. However, the autocorrelation curves of eGFP- and Dreiklanglabeled Na,K-ATPase measured in the plasma membrane exhibit marked differences, with the Dreiklang-labeled construct showing shorter diffusion times. This may be related to an additional, as yet unrecognized quenching process that occurs on the same time scale as the diffusion of the labeled complexes through the detection volume (1– 100 ms). Since the origin of this quenching process is currently unclear, care has to be taken when the Dreiklang label is intended to be used in FCS applications.
Atomic weights of the elements 2013 (IUPAC Technical Report)
Authors: Meija Juris , Coplen Tyler B. , Berglund Michael , Brand Willi A. , De Bièvre Paul , Gröning Manfred , Holden Norman E. , Irrgeher Johanna , Loss Robert D. , Walczyk Thomas , Prohaska Thomas 
Published: March 2016

Abstract
The biennial review of atomic-weight determinations and other cognate data has resulted in changes for the standard atomic weights of 19 elements. The standard atomic weights of four elements have been revised based on recent determinations of isotopic abundances in natural terrestrial materials:

cadmium to 112.414(4) from 112.411(8),

molybdenum to 95.95(1) from 95.96(2),

selenium to 78.971(8) from 78.96(3), and

thorium to 232.0377(4) from 232.038 06(2).

The Commission on Isotopic Abundances and Atomic Weights (ciaaw.org) also revised the standard atomic weights of fifteen elements based on the 2012 Atomic Mass Evaluation:

aluminium (aluminum) to 26.981 5385(7) from 26.981 5386(8),

arsenic to 74.921 595(6) from 74.921 60(2),

beryllium to 9.012 1831(5) from 9.012 182(3),

caesium (cesium) to 132.905 451 96(6) from 132.905 4519(2),

cobalt to 58.933 194(4) from 58.933 195(5),

fluorine to 18.998 403 163(6) from 18.998 4032(5),

gold to 196.966 569(5) from 196.966 569(4),

holmium to 164.930 33(2) from 164.930 32(2),

manganese to 54.938 044(3) from 54.938 045(5),

niobium to 92.906 37(2) from 92.906 38(2),

phosphorus to 30.973 761 998(5) from 30.973 762(2),

praseodymium to 140.907 66(2) from 140.907 65(2),

scandium to 44.955 908(5) from 44.955 912(6),

thulium to 168.934 22(2) from 168.934 21(2), and

yttrium to 88.905 84(2) from 88.905 85(2).

The Commission also recommends the standard value for the natural terrestrial uranium isotope ratio, N(238U)/N(235U)=137.8(1).
Thin film flow of an Oldroyd 6-constant fluid over a moving belt: an analytic approximate solution
Authors: Ene Remus-Daniel , Marinca Vasile , Marinca Valentin Bogdan 
Published: March 2016

Abstract
In this paper the thin film flow of an Oldroyd 6-constant fluid on a vertically moving belt is investigated. The basic equation of a non-Newtonian fluid in a container with a wide moving belt which passes through the container moving vertically upward with constant velocity, is reduced to an ordinary nonlinear differential equation. This equation is solved approximately by means of the Optimal Homotopy Asymptotic Method (OHAM). The solutions take into account the behavior of Newtonian and non-Newtonian fluids. Our procedure intended for solving nonlinear problems does not need small parameters in the equation and provides a convenient way to control the convergence of the approximate solutions.
Lysosomes appear as the auto-fluorescent vacuoles in Dictyostelium discoideum cells
Authors: Kainuma Hiroki , Saito Yuya , Hatakeyama Ikuo , Omata Takako A. , Uchiyama Saburo 
Published: March 2016

Abstract

Dictyostelium discoideum cells contain auto-fluorescent vacuoles. To determine the identity of these vacuoles, the fluorescent dye 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) was used to stain the lysosomes in D. discoideum cells. Neither the auto-fluorescent vacuoles nor lysosomes were observed in D. discoideum cells immediately after they arose from spores or in stationary phase cells. However, both the auto-fluorescent vacuoles and lysosomes were visible in cells that had entered growth phase. Auto-fluorescent vacuoles and lysosomes were also observed in stationary phase cells incubated with chloroquine. When the cells were allowed to phagocytose BioParticles Fluorescent Bacteria (orange fluorescence) for 1 h, orange phagosomes and blue auto-fluorescent vacuoles were observed as independent moieties. However, after an additional 2 h of incubation, we observed vacuoles with mixed fluorescence (orange and blue) in the cells, suggestive of secondary lysosomes. These results suggest that the auto-fluorescent vacuoles in D. discoideum cells are lysosomes.
The dative of agent in Indo-European languages
Authors: Luraghi Silvia 
Published: March 2016

AbstractThe paper discusses various constructions of ancient Indo-European languages that have been described as featuring a dative of agent. The occurrence of the dative can be explained either through its beneficiary meaning, or as indicating an experiencer. In fact, a number of passages that have been taken as evidence for the reconstruction of a dative of agent do not contain agent phrases at all. Thus, while different constructions have parallels in two or more ancient languages, it is impossible to reconstruct a dative of agent as a unified category for PIE, except in the case of nominal verb forms denoting obligation.
Dem Herrgott sei Scheenster
Authors: Nickel Grit 
Published: March 2016

AbstractBy integrating dialect data into typological theses on dative-marked nominal possessive constructions of the typedem Vater sein Hut, this article will continue recent approaches of both dialectological and typological research. By means of an empirical study, this article intends to not only describe the surface phenomena, but to uncover the underlying structure of this construction in East Franconian dialects. Language maps illustrate that instances of microvariation concerning the case-marking of the possessor (Wemvs.Wenvs.Wer seine Weiden sind das?) and the form of the possessive pronoun (meiner Tante ihrvs.sein Wirsing) are diatopically conditioned.
Molecular structure studies of novel bronchodilatory-active 4-azafluorenes
Authors: Girgis Adel S. , Aziz Marian N. , Shalaby ElSayed M. , Saleh Dalia O. , Mishriky Nawal , El-Eraky Wafaa I. , Farag I.S. Ahmed 
Published: March 2016

Abstract
Two 5H-indeno[1,2-b]pyridines, 7a and 7b, were synthesized and characterized by X-ray crystallography. In the molecular packing, molecules of 7a are linked into chains by C–H···N hydrogen bond which, in turn, are connected by H···π, N···π, Cl···π and π···π interactions. In the crystal structure of 7b, molecules are connected by C–H···N and C–H···Cl interactions as well as a set of N···π and Cl···π interactions. The molecular structures were studied by theory using AM1, PM3 and DFT. The basic difference between the theoretical and experimental structures was found in the relative orientation of dichlorophenyl ring attached to the indenopyridine residue, which was revealed to be aligned in nearly opposite orientations. This observation is attributed to the bulky chlorine atom(s) of the phenyl ring that prevent free rotation around the sigma bond attaching this ring with the heterocyclic system. DFT was used to determine the molecular electrostatic potential revealing the nitrile nitrogen to be the most nucleophilic site. A low HOMO-LUMO energy gap indicates high reactivity of 7a and 7b. The synthesized azafluorenes show more potent bronchodilation properties than the standard reference compound (theophylline).
Disbursing the Account of God: Fiscal Terminology and the Economy of God in Colossians 1,24–25
Authors: Lang T.J. 
Published: March 2016

Abstract:
Die Schwierigkeiten bei der Interpretation des irritierenden Satzes in Kol 1,24 – „ich fülle auf, was an den Leiden Christi noch fehlt“ (ἀνταναπληρῶ τὰ ὑστερήματα τῶν θλίψεων τοῦ Χριστοῦ) – sind bekannt. Die Beziehung dieses Satzes zum größeren Kontext von Kol 1,24–2,3 und insbesondere zur parallelen Wendung mit πληρόω in V.25 wird jedoch weit weniger bemerkt. In diesem Aufsatz wird die These vertreten, dass die für gewöhnlich mit „auffüllen, was an den Leiden Christi noch fehlt“ übersetzte Wendung in V.24 ein terminus technicus aus dem Finanzwesen ist, der anzeigt, dass den Völkern der für sie durch Christus entstandene Reichtum (V.27) durch die Anstrengungen des Paulus in seinem apostolischen Dienst zugeteilt wird. Daher bleibt nicht der soteriologische Reichtum, der durch Christus entstanden ist, unzulänglich, sondern die persönliche „Überweisung“ dieses „christologischen Kapitals“ an die Völker, für die es be stimmt war. Dieses Bild aus der Finanzwirtschaft wird dann am Ende von V.25 umgestaltet, wo Paulus den Zweck der οἰκονομία definiert, die er von Gott erhalten hat: Die finanzielle Verantwortung, die Paulus trägt, ist, „das Konto Gottes auszubezahlen“ (πληρῶσαι τὸν λόγον τοῦ θεοῦ). Das Ausbezahlen „christologischer Reichtümer“ von Gottes Konto ist genau das, was man von einem „Manager“ (διάκονος) mit treuhänderischer Pflicht (οἰκονομία) erwarten würde.
How to make a synaptic ribbon: RIBEYE deletion abolishes ribbons in retinal synapses and disrupts neurotransmitter release
Authors: Maxeiner Stephan , Luo Fujun , Tan Alison , Schmitz Frank , Südhof Thomas C 
Published: March 2016

AbstractSynaptic ribbons are large proteinaceous scaffolds at the active zone of ribbon synapses that are specialized for rapid sustained synaptic vesicles exocytosis. A single ribbon‐specific protein is known, RIBEYE, suggesting that ribbons may be constructed from RIBEYE protein. RIBEYE knockdown in zebrafish, however, only reduced but did not eliminate ribbons, indicating a more ancillary role. Here, we show in mice that full deletion of RIBEYE abolishes all presynaptic ribbons in retina synapses. Using paired recordings in acute retina slices, we demonstrate that deletion of RIBEYE severely impaired fast and sustained neurotransmitter release at bipolar neuron/AII amacrine cell synapses and rendered spontaneous miniature release sensitive to the slow Ca2+‐buffer EGTA, suggesting that synaptic ribbons mediate nano‐domain coupling of Ca2+ channels to synaptic vesicle exocytosis. Our results show that RIBEYE is essential for synaptic ribbons as such, and may organize presynaptic nano‐domains that position release‐ready synaptic vesicles adjacent to Ca2+ channels.
CPAP promotes timely cilium disassembly to maintain neural progenitor pool
Authors: Gabriel Elke , Wason Arpit , Ramani Anand , Gooi Li Ming , Keller Patrick , Pozniakovsky Andrei , Poser Ina , Noack Florian , Telugu Narasimha Swamy , Calegari Federico , Šarić Tomo , Hescheler Jürgen , Hyman Anthony A , Gottardo Marco , Callaini Giuliano , Alkuraya Fowzan Sami , Gopalakrishnan Jay 
Published: March 2016

AbstractA mutation in the centrosomal‐P4.1‐associated protein (CPAP) causes Seckel syndrome with microcephaly, which is suggested to arise from a decline in neural progenitor cells (NPCs) during development. However, mechanisms of NPCs maintenance remain unclear. Here, we report an unexpected role for the cilium in NPCs maintenance and identify CPAP as a negative regulator of ciliary length independent of its role in centrosome biogenesis. At the onset of cilium disassembly, CPAP provides a scaffold for the cilium disassembly complex (CDC), which includes Nde1, Aurora A, and OFD1, recruited to the ciliary base for timely cilium disassembly. In contrast, mutated CPAP fails to localize at the ciliary base associated with inefficient CDC recruitment, long cilia, retarded cilium disassembly, and delayed cell cycle re‐entry leading to premature differentiation of patient iPS‐derived NPCs. Aberrant CDC function also promotes premature differentiation of NPCs in Seckel iPS‐derived organoids. Thus, our results suggest a role for cilia in microcephaly and its involvement during neurogenesis and brain size control.
Comparison of Clinical Risk Factors Among Pediatric Patients With Single Admission, Multiple Admissions (Without Any 7-Day Readmissions), and 7-Day Readmission
Authors: Winer Jeffrey C. , Aragona Elena , Fields Alan I. , Stockwell David C. 
Published: March 2016

BACKGROUND AND OBJECTIVE:
Readmissions have received increasing attention. The goal of this study was to identify demographic and clinical factors associated with hospital utilization and 7-day readmissions.

METHODS:
This retrospective case–control study of inpatient and observation encounters was conducted at a freestanding children’s hospital. Over a 1-year period, patients were categorized into 3 groups: patients with a single admission, patients with multiple admissions without any 7-day readmissions, and patients with at least one 7-day readmission. Factors associated with risk of future hospital utilization were determined, and post hoc testing was performed to compare groups.

RESULTS:
Patients with a single admission had statistically significant lower numbers of medications at admission and discharge, lower rates of home health care at admission and discharge, and fewer diagnosis codes during index admission than patients with multiple admissions. There were no statistically significant differences among patients with multiple admissions between those with and without 7-day readmissions.

CONCLUSIONS:
This study found that patients with multiple admissions were similar regardless of whether they had any 7-day readmissions. Because patients with multiple admissions seemed to represent a single high-risk group, it is possible that many readmissions represent a consequence of medical complexity rather than a failure of care. Further studies are necessary to determine if providing targeted interventions to high-risk patients will lower their future hospital utilization.
Implementation of an Innovative Pediatric Hospital Medicine Education Series
Authors: Statile Angela M. , Unaka Ndidi , Thomson Joanna E. , Sucharew Heidi , Gonzalez del Rey Javier , White Christine M. 
Published: March 2016

OBJECTIVE:
Limitations on resident duty hours require formal education programs to be high-yield and impactful. Hospital medicine (HM) topics provide the foundation for inpatient pediatric knowledge pertinent to pediatric residents and medical students. Our primary objective was to describe the creation of an innovative pediatric HM curriculum designed to increase learners’ medical knowledge and their confidence in communicating with patients and families about these topics; our secondary objective was to evaluate the level of innovation of the conference sessions perceived by the learners.

METHODS:
A systematic approach was used to develop a curriculum framework incorporating a variety of interactive and engaging educational strategies. Six sessions were studied over the 2012–2013 academic year. The bimonthly sessions were presented during the resident daily conference schedule as a recurring pediatric HM series. Change in learners’ medical knowledge and confidence in communicating with families were analyzed presession to postsession by using McNemar’s test and the Wilcoxon signed rank test, respectively. Learners rated the level of innovation for each session on a 5-point Likert scale.

RESULTS:
Content covered during the 6 sessions included bronchiolitis, child abuse, health care systems, meningitis/fever, urinary tract infection, and wheezing. Medical knowledge increased presession to postsession (P &lt; .001), as did confidence in communicating about each topic with families (P &lt; .01). The average rating score for all sessions was highly innovative.

CONCLUSIONS:
A systematic approach is useful for developing new curricula for pediatric learners. Focusing on high-yield topics and established competencies allows impactful education sessions within the confines of pediatric learners’ schedule constraints.
The Influence of Comorbid Mood and Anxiety Disorders on Outcomes of Pediatric Patients Hospitalized for Pneumonia
Authors: Doupnik Stephanie K. , Mitra Nandita , Feudtner Chris , Marcus Steven C. 
Published: March 2016

OBJECTIVES:Mood and anxiety disorders are associated with greater inpatient care utilization in children with chronic illness. We sought to investigate the association of mood or anxiety disorders and outcomes for hospitalized pediatric patients, using pneumonia as a model.METHODS:We conducted a retrospective, cross-sectional study of pneumonia hospitalizations in patients 5 to 20 years old, using the nationally representative Healthcare Cost and Utilization Project’s 2012 Kids’ Inpatient Database. We used multivariable logistic and linear regression models stratified by age group to determine the independent association of mood or anxiety disorders with complications and length of stay, adjusted for clinical, demographic, and hospital characteristics.RESULTS:Of 34 794 pneumonia hospitalizations, 3.5% involved a patient with a comorbid mood or anxiety disorder. Overall incidence of complications was 13.1%. Mean length of stay was 4.5 days. In adjusted models, comorbid mood or anxiety disorders were associated with greater odds of pneumonia complications in school-aged children (odds ratio 1.80; 95% confidence interval, 1.20–2.71) and adolescents (odds ratio 1.63; 95% confidence interval, 1.31–2.02). Hospitalizations with an associated mood or anxiety disorder were longer than those without, by 11.2% in school-aged children and 13.6% in adolescents (P &lt; .001). The association of mood and anxiety disorders with longer hospital stay was not modified by the presence of pneumonia complications.CONCLUSIONS:In pediatric patients hospitalized for pneumonia, a comorbid mood or anxiety disorder is associated with greater odds of complications and longer hospital stay. The presence of pneumonia complications did not influence the relationship between mood or anxiety disorders and length of stay.
Una mirada personalista de la obra Pinocho de Collodi: ser persona, la libertad, el vértigo y el amor
Authors: Quesada Mora Gabriel 
Published: March 2016

<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>El presente trabajo estudiará la obra </span><span>Las aventuras de Pinocho </span><span>del es- </span><span>critor italiano Carlo Collodi a partir del marco filosófico de la escuela </span><span>personalista desarrollada en el siglo XX. Se hará énfasis en los conceptos </span><span>de persona humana, de libertad y del amor como vocación y finalidad de las personas. Se analizará en su conjunto la propuesta axiológica de </span><span>la obra. Como apoyo complementario de análisis, se utilizará el método </span><span>lúdico-ambital </span><span>del filósofo español Alfonso López Quintás para dilucidar </span><span>las distinciones entre </span><span>persona </span><span>y </span><span>objeto </span><span>(el primero como fin en sí mismo y </span><span>el segundo como medio); </span><span>sentido </span><span>y </span><span>significado </span><span>(lo esencial y exclusivo vrs. lo inmediato y denotativo); el concepto de </span><span>ámbito </span><span>(capacidad de fundar realidades creativas) y de </span><span>encuentro </span><span>(entre los seres en el que la libertad </span><span>es primordial); y las categorías de </span><span>vértigo </span><span>(posesivo y violento) y </span><span>éxta- sis </span><span>(apertura y elevación por el amor); </span><span>acontecimiento </span><span>y </span><span>hecho histórico</span><span>. En su conjunto, este trabajo propone un </span><span>personalismo literario </span><span>en el que </span><span>convergen el arte de la ficción y el arte del pensamiento filosófico. Uno </span><span>de los principales aportes de este trabajo es mostrar la utilidad de las </span><span>categorías filosóficas del personalismo para analizar las obras literarias. </span><span>En el caso concreto de </span><span>Pinocho </span><span>se estudiará cómo, a pesar de iniciar expe</span><span>- </span><span>riencias de vértigo, desenfreno, manipulación y cosificación de los seres; </span><span>Pinocho se transforma en un verdadero ser humano en el que la libertad y el amor tienen la última palabra. </span></p></div></div></div>
Diversidad en percepciones de la problemática social en el continente americano: cruzando fronteras mediante composiciones musicales
Authors: Pacheco Acuña Gilda 
Published: March 2016

<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>El siguiente artículo utiliza canciones de diferentes regiones de América </span><span>escritas al final de la década de los años ochentas por cantautores re</span><span>- </span><span>presentativos de Argentina, Costa Rica y Estados Unidos. Estos textos, productos de cultura popular, son analizados como poemas y para dicho estudio se usan tanto estrategias de los enfoques comparativos literarios como premisas de los estudios culturales. Con esta propuesta teórica se busca visualizar interesantes similitudes y señalar importantes diferen</span><span>- </span><span>cias en estas producciones textuales desde diversos contextos históricos, políticos y sociales de los países seleccionados. </span></p></div></div></div>
La mujer “real”: cuerpos e historias particulares en Real Women Have Curves de Josefina López
Authors: López Get Anthony 
Published: March 2016

<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>Este artículo analiza las relaciones de poder existentes entre los sujetos </span><span>“hombres” y “mujeres” en la obra de Josefina López </span><span>Real Women Have Curves. </span><span>Desde un enfoque feminista poscolonial, se estudia la domina</span><span>ción masculina sobre el cuerpo de las mujeres – cuerpos colonizados –, así </span><span>como la necesidad, planteada en la obra, de un estudio feminista multi</span><span>cultural – no etnocéntrico–, incluyente, que tome en cuenta las particula</span><span>ridades de las mujeres doblemente marginadas en tanto mujeres del Ter- </span><span>cer Mundo en oposición a una identidad de mujer absoluta y esencialista. </span></p></div></div></div>