bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design, synthesis, and cytotoxic evaluation of novel furo[2,3‐b]quinoline derivatives
Authors: Wang Bing , Li Qiangqiang , Shi Wei , Chen Li , Sun Jianbo 
Published: December 2017

A number of novel furo[2,3‐b]quinoline derivatives were designed and synthesized by introducing benzyl ether, benzoate, and benzenesulfonate to 6‐position of furo[2,3‐b]quinoline and their chemical structures were confirmed by ESI‐MS, 1H NMR, and 13C NMR spectra. All target compounds were evaluated in vitro against four human cancer cell lines (HCT‐116, MCF‐7, U2OS, and A549) by MTT method. Cytotoxic assay showed that compounds 8a, 8e, 10a, 10b, and 10c exhibited more potent cytotoxicities compared to 2‐bromine‐6‐hydroxy‐furo‐[2,3‐b]quinoline (7). Compound 10c exhibited higher antiproliferative activity (IC50 values ranging from 4.32 to 24.96 μm) against four human cancer cell lines (HCT‐116, MCF‐7, U2OS, and A549) and weak cytotoxicity on normal cell HL‐7702, which suggested that 10c might be an ideal anticancer candidate. Compounds 8a, 10a, 10b showed good selectivities to MCF‐7 and HCT‐116, which could be considered as ideal selective candidates for further study. The SARs showed that the introduction of the benzyl ether and benzenesulfonate could significantly improve cytotoxicities, while the benzoate failed to enhance potency obviously.
Impact of Austropuccinia psidii in New Caledonia, a biodiversity hotspot
Authors: Soewarto J. , Carriconde F. , Hugot N. , Bocs S. , Hamelin C. , Maggia L. 
Published: December 2017

SummaryMyrtle rust, caused by the fungus Austropuccinia psidii, infects a wide range of host species within the Myrtaceae family worldwide. Since its first report in 2013 from New Caledonia, the extent of the host range, geographical distribution and genetic diversity of A. psidii in this territory have been unknown. This study reports 67 new host species distributed in 13 different Myrtaceae genera, including five new genera. The pathogen was found in various types of plant communities, where Myrtaceae species are the dominant or codominant species, as well as in several nurseries. It is now considered a significant threat to the biodiversity of ecosystems and the Myrtaceae‐related economy. A 3‐year disease monitoring trial with 35 cultivated Myrtaceae species showed variations in disease severity at the species and individual scales. This suggests that some level of genetic resistance to myrtle rust may possibly exist among the endemic Myrtaceae of New Caledonia. A population genetic investigation revealed that only one single genotype of A. psidii occurs across its entire host range in New Caledonia. Therefore, it is essential to carry out population genetic surveillance to identify the appearance of mutations or the introduction of potentially more virulent genotypes of A. psidii. The outcomes from this study will assist with the ongoing management of the disease in New Caledonia.
Protection of macrophages from intracellular pathogens by miR‐182‐5p mimic—a gene expression meta‐analysis approach
Authors: Gregory David J. , Kramnik Igor , Kobzik Lester 
Published: December 2017

The goals of this study were to (a) define which host genes are of particular importance during the interactions between macrophages and intracellular pathogens, and (b) use this knowledge to gain fresh, experimental understanding of how macrophage activities may be manipulated during host defense. We designed an in silico method for meta‐analysis of microarray gene expression data, and used this to combine data from 16 different studies of cells in the monocyte–macrophage lineage infected with seven different pathogens. Three thousand four hundred ninety‐eight genes were identified, which we call the macrophage intracellular pathogen response (macIPR) gene set. As expected, the macIPR gene set showed a strong bias toward genes previously associated with the immune response. Predicted target sites for miR‐182‐5p (miR‐182) were strongly over‐represented among macIPR genes, indicating an unexpected role for miR‐182‐regulatable genes during intracellular pathogenesis. We therefore transfected primary human alveolar macrophage‐like monocyte‐derived macrophages from multiple different donors with synthetic miR‐182, and found that miR‐182 overexpression (a) increases proinflammatory gene induction during infection with Francisella tularensis live vaccine strain (LVS), (b) primes macrophages for increased autophagy, and (c) enhances macrophage control of both gram negative F. tularensisLVS and gram positive Bacillus anthracisANR‐1 spores. These data therefore suggest a new application for miR‐182 in promoting resistance to intracellular pathogens.
Observations on the origin of ovarian cortical inclusion cysts in women undergoing risk‐reducing salpingo‐oophorectomy
Authors: Park Kay J , Patel Prusha , Linkov Irina , Jotwani Anjali , Kauff Noah , Pike Malcolm C 
Published: December 2017

AimsEvidence suggests that up to 70% of high‐grade serous ovarian carcinomas (HGSCs) arise potentially from fallopian tube fimbriae, and that many of the remaining cases arise from within the ovary in cortical inclusion cysts (CICs) with a Müllerian phenotype (Müllerian‐CICs). It has been proposed that Müllerian‐CICs arise either from metaplasia of mesothelial ovarian surface epithelium (OSE) entrapped within the ovary after ovulation or from normal tubal cells entrapped postovulation. However, this proposal is controversial. We therefore conducted a study of CICs in women, most of them BRCA1/2 mutation carriers, undergoing risk‐reducing salpingo‐oophorectomy at our institution from 2000 to 2014.Methods and resultsWe used immunohistochemistry for PAX8, a Müllerian marker, and calretinin, a mesothelial marker to classify CIC cells. In 499 CICs from 59 women, 72.3% were positive for PAX8 (PAX8+): ≥10% of CIC cells positive; 43.5% positive for calretinin (calretinin+). The proportion of PAX8+ CICs increased from 62.9% in premenopausal to 80.5% in postmenopausal patients. The proportion of calretinin+ CICs decreased from 52.6% to 35.6%, respectively. There was significant overlap of PAX8 and calretinin positivity: 82 (16.4%) CICs were PAX8+/calretinin+; 43 (40.2%) of these 82 demonstrated PAX8+/calretinin+ in the same cells.ConclusionsThese results, and the increased ratio of PAX8+ to calretinin+ CICs from premenopausal to postmenopausal, show that many PAX8+ CICs probably arise from metaplasia of OSE‐derived CICs. The proportion of PAX+/calretinin− CICs arising from OSE‐derived CICs is unclear, but our results strongly support the proposal that many Müllerian‐CICs arise from OSE via metaplasia.
Bullous pemphigoid complicated by cytomegalovirus disease as a manifestation of immune reconstitution inflammatory syndrome: retrospective analyses of our institutional cases and literature review
Authors: Narita Yoko M. , Horie Chiho , Hirahara Kazuhisa , Kano Yoko , Shiohara Tetsuo , Mizukawa Yoshiko 
Published: December 2017

AbstractBackgroundCytomegalovirus (CMV) disease induced by reactivation of latent CMV is a fatal viral infection that may develop in a setting of therapy with immunosuppressive agents. There is a clear need to clarify any clinical features and markers of CMV disease.ObjectiveWe investigated which clinical markers usually available in a clinical setting can predict CMV disease occurring in bullous pemphigoid (BP) patients receiving corticosteroids.MethodWe described a BP patient with CMV disease complicated by gastrointestinal hemorrhage and liver dysfunction. Prompted by this patient, we retrospectively analyzed clinical features and laboratory findings in our institutional four BP patients and previously reported nine BP patients with CMV disease. We also compared these patients with our institutional 42 BP patients not complicated by CMV disease.ResultsHigh levels of anti‐BP180 antibody titers associated with resistance to corticosteroids are a risk factor for the development of CMV disease. A reduction in platelet (PLT) and white blood cell (WBC) counts and an increase in alanine aminotransferase (ALT) levels 3–4 weeks after the initiation of corticosteroids are useful predictive markers for the onset of CMV disease.ConclusionsFrequent WBC, PLT, and ALT measurements may identify BP patients at a risk of subsequently developing CMV disease. Careful monitoring of CMV disease in BP refractory to systemic corticosteroids may reduce the risk of fatal outcomes.
Cardiopulmonary anomalies in incontinentia pigmenti patients
Authors: Onnis Giuliana , Diociaiuti Andrea , Zangari Paola , D'Argenio Patrizia , Cancrini Caterina , Iughetti Lorenzo , El Hachem May 
Published: December 2017

AbstractBackgroundIncontinentia pigmenti (IP) is a rare inherited genodermatosis that usually involves the skin, and also teeth, oral cavity, central nervous system, eyes, blood with eosinophilia, and rarely skeletal system, breast, heart, and lungs. Skin lesions usually appear early, at birth or within the first 2 weeks of life, with four different phases tending to follow Blaschko lines that may overlap.Case reportWe report a rare case of a neonate with transient reversible pulmonary hypertension that presented at day 9 of life. She manifested increasing dyspnea and deterioration of respiratory dynamics with a serious pulmonary hypertension without a primary pulmonary disease. Hence, oxygen therapy at high flows and nitric oxide have been administered with an initial response, but, subsequently, because of the worsening of the respiratory activity, she underwent sildenafil and bosentan treatment with respiratory dynamics improvement and progressive decrease of the pulmonary pressures.ConclusionIn literature only a few cases of cardiopulmonary anomalies in IP have been described with different outcomes, and these rare complications are probably underestimated by physicians. We could suppose that microangiopathic damages may have a critical role in endothelial alterations, and these processes are probably shared by multiple organs involved in IP and rarely by lungs and heart.
Impact of high‐pressure processing on chemical constituents and nutritional properties in aquatic foods: a review
Authors: Aubourg Santiago P. 
Published: December 2017

SummaryAmong the recent advanced technologies, high‐pressure processing (HPP) has emerged as an attractive tool for inactivating microbial populations and endogenous enzymes activity, while retaining the sensory and nutritional properties. This review covers information concerning the research carried out on the changes undergone by the different chemical constituents of aquatic foods as a result of HPP, including modifications produced in nutritional value parameters. Such description includes changes produced just after the HPP as well as those resulting from a combination of HPP and further storage (i.e. refrigerated and frozen storages) or processing (thermal treatment, ready‐to‐eat products elaboration, etc.) corresponding to common commercial procedures employed nowadays by the food industry. The impact of constituent modifications on the formation of molecules related to quality parameters is also reviewed. Finally, the needs for complementary research on constituent changes by HPP and the possibilities for widening the application of this advanced technology are discussed.
Statistical mechanical model of bonding in mixed modifier glasses
Authors: Goyal Sushmit , Mauro John C. 
Published: December 2017

AbstractOxide glasses often consist of multiple network formers that create the backbone of the glass network and modifiers that serve as either charge compensators or creators of non‐bridging oxygens. The variety of bonding preferences results in very rich composition‐property relationships. In this work, we present a statistical description of the glass structure governed by the relative enthalpic and entropic contributions to the bonding preferences in a glassy system. Using the proposed model, we derive an analytical expression to represent the bonding in mixed modifier glasses and explain the role of composition and fictive temperature on glass structure. The model provides the criteria for nonlinearity in bonding preference and reveals regions where high fluctuations in local structure are predicted.
A longitudinal investigation of the role of parental responses in predicting children's post‐traumatic distress
Authors: Hiller Rachel M. , Meiser‐Stedman Richard , Lobo Sarah , Creswell Cathy , Fearon Pasco , Ehlers Anke , Murray Lynne , Halligan Sarah L. 
Published: December 2017

BackgroundWhile parental post‐trauma support is considered theoretically important for child adjustment, empirical evidence concerning the specific aspects of parental responding that influence child post‐traumatic distress, or the processes via which any such impacts occur, is extremely limited. We conducted a longitudinal examination of whether parental post‐trauma appraisals, trauma‐specific support style and general parenting style predicted child post‐traumatic stress symptom severity (PTSS) following trauma; and whether such influences operated via the child's own appraisals and coping style.MethodWe recruited 132 parent–child pairs following children's experience of acute trauma. We examined whether parental responses assessed at 1‐month post‐trauma, predicted child PTSS at 6‐month follow‐up. Parental trauma‐specific appraisals and responses, and general parenting style, were assessed via both self‐report and direct observations. Child‐report questionnaires were used to assess PTSS and potential mediators.ResultsInitial parent negative appraisals and encouragement of avoidant coping were associated with higher child‐reported PTSS at 6‐month follow‐up. Predictive effects were maintained even when controlling for initial child symptom levels. Observational assessments broadly supported conclusions from self‐report. There was evidence that parental influences may operate, in part, by influencing the child's own appraisals and coping responses. In contrast, there was no evidence for an influence of more “adaptive” support or general parenting style on child PTSS.ConclusionsFindings provide important insight into how elements of social support may influence child post‐trauma outcomes.
The high societal costs of childhood conduct problems: evidence from administrative records up to age 38 in a longitudinal birth cohort
Authors: Rivenbark Joshua G. , Odgers Candice L. , Caspi Avshalom , Harrington HonaLee , Hogan Sean , Houts Renate M. , Poulton Richie , Moffitt Terrie E. 
Published: December 2017

BackgroundChildren with conduct problems that persist into adulthood are at increased risk for future behavioral, health, and social problems. However, the longer term public service usage among these children has not been fully documented. To aid public health and intervention planning, adult service usage across criminal justice, health care, and social welfare domains is compared among all individuals from a representative cohort who followed different conduct problem trajectories from childhood into adulthood.MethodsParticipants are from the Dunedin Multidisciplinary Health and Development Study, a prospective, representative cohort of consecutive births (N = 1,037) from April 1972 to March 1973 in Dunedin, New Zealand. Regression analyses were used to compare levels of public service usage up to age 38, gathered via administrative and electronic medical records, between participants who displayed distinct subtypes of childhood conduct problems (low, childhood‐limited, adolescent‐onset, and life‐course persistent).ResultsChildren exhibiting life‐course persistent conduct problems used significantly more services as adults than those with low levels of childhood conduct problems. Although this group comprised only 9.0% of the population, they accounted for 53.3% of all convictions, 15.7% of emergency department visits, 20.5% of prescription fills, 13.1% of injury claims, and 24.7% of welfare benefit months. Half of this group (50.0%) also accrued high service use across all three domains of criminal justice, health, and social welfare services, as compared to only 11.3% of those with low conduct problems (OR = 7.27, 95% CI = 4.42–12.0).ConclusionsConduct problems in childhood signal high future costs in terms of service utilization across multiple sectors. Future evaluations of interventions aimed at conduct problems should also track potential reductions in health burden and service usage that stretch into midlife.
Cost‐effectiveness of cognitive therapy as an early intervention for post‐traumatic stress disorder in children and adolescents: a trial based evaluation and model
Authors: Shearer James , Papanikolaou Nestor , Meiser‐Stedman Richard , McKinnon Anna , Dalgleish Tim , Smith Patrick , Dixon Clare , Byford Sarah 
Published: December 2017

BackgroundUntreated post‐traumatic stress disorder (PTSD) in children and adolescents is associated with a considerable economic burden on the health system, families and society. Recent research has demonstrated the potential efficacy of cognitive therapy as an early intervention for PTSD in children and adolescents. Children who experienced a single traumatic event in the previous two to six months and were randomized to cognitive therapy for PTSD (CT‐PTSD) were significantly more likely to be PTSD‐free compared to those randomized to usual care represented by waitlist control. The current study evaluated the economic impact of improvements in the treatment of PTSD in children and adolescents.MethodsA cost‐effectiveness analysis was conducted from the national health service/personal social services perspective with outcomes expressed as quality‐adjusted life years (QALYs). Patient level costs and outcomes were collected during the 11 week clinical trial and extrapolated to a three year time horizon using economic modelling methods. Uncertainty was estimated using probabilistic sensitivity analysis and assumptions were tested using one way sensitivity analysis.ResultsThe incremental cost‐effectiveness ratio at 3 years was £2,205 per QALY with a 60%–69% probability of CT‐PTSD being cost‐effective compared to usual care at the UK £20,000 to £30,000 per QALY decision threshold.ConclusionsThis study provides preliminary evidence for the cost‐effectiveness of cognitive therapy in this treatment population. Larger pragmatic trials with longer follow‐up are indicated.
Prospective test of the developmental propensity model of antisocial behavior: from childhood and adolescence into early adulthood
Authors: Lahey Benjamin B. , Class Quetzal A. , Zald David H. , Rathouz Paul J. , Applegate Brooks , Waldman Irwin D. 
Published: December 2017

BackgroundThe developmental propensity model of antisocial behavior posits that several dispositional characteristics of children transact with the environment to influence the likelihood of learning antisocial behavior across development. Specifically, greater dispositional negative emotionality, greater daring, and lower prosociality—operationally, the inverse of callousness— and lower cognitive abilities are each predicted to increase risk for developing antisocial behavior.MethodsProspective tests of key predictions derived from the model were conducted in a high‐risk sample of 499 twins who were assessed on dispositions at 10–17 years of age and assessed for antisocial personality disorder (APD) symptoms at 22–31 years of age. Predictions were tested separately for parent and youth informants on the dispositions using multiple regressions that adjusted for oversampling, nonresponse, and clustering within twin pairs, controlling demographic factors and time since the first assessment.ResultsConsistent with predictions, greater numbers of APD symptoms in adulthood were independently predicted over a 10–15 year span by higher youth ratings on negative emotionality and daring and lower youth ratings on prosociality, and by parent ratings of greater negative emotionality and lower prosociality. A measure of working memory did not predict APD symptoms.ConclusionsThese findings support future research on the role of these dispositions in the development of antisocial behavior.
Potassium phosphites in the protection of common bean plants against anthracnose and biochemical defence responses
Authors: Costa Bruno Henrique Garcia , de Resende Mário Lúcio Vilela , Monteiro Ana Cristina Andrade , Ribeiro Júnior Pedro Martins , Botelho Deila Magna dos Santos , Silva Bruno Marques da 
Published: December 2017

AbstractThe aim of this study was to investigate the effectiveness of potassium phosphites for the control of anthracnose and the mode of action of these products on common bean plants against Colletotrichum lindemuthianum, comparing it with the standard resistance inducer acibenzolar‐S‐methyl. The protection of plants against anthracnose was evaluated in greenhouse after treatment with potassium phosphites (Phosphite A and B, 5.0 ml/L), acibenzolar‐S‐methyl (0.25 g/L), or no treatment (control). Two sprayings of the treatments were performed, respectively, at V4 stage (three trifoliate leaves) and at the R5 stage (flower buds present). The inoculation with C. lindemuthianum was performed 5 days after the first spraying. Phosphite formulations A and B reduced the severity of anthracnose by 68.7% and 55.6%, respectively, and the presence of phosphites in the leaf tissues were detected at concentrations between 1 and 3 mm by 7 days after spraying. These same concentrations of phosphites reduced the mycelial growth of C. lindemuthianum in vitro by 15.0% to 25.7%. In addition, the activities of defence enzymes and the levels of phenolic compounds and lignin were assessed. Phosphite treatments enhanced the activity of various enzymes, including superoxide dismutase, peroxidase, chitinase, and β‐1,3‐glucanase, and increased the lignin and a small increase in the levels of soluble phenolics. This study provides evidence that phosphite treatments control anthracnose by acting directly on C. lindemuthianum and by inducing the production of defence responses.
Phylogeny, morphology and pathogenicity of Elsinoë ampelina, the causal agent of grapevine anthracnose in Brazil and Australia
Authors: Santos Ricardo Feliciano dos , Spósito Marcel Bellato , Ayres Matthew Ronald , Sosnowski Mark Roman 
Published: December 2017

AbstractAnthracnose caused by Elsinoë ampelina is one of the most important table grape diseases in humid regions in Brazil and Australia. The objective of this study was to characterize E. ampelina isolates from Brazil and Australia by means of phylogenetic analyses, morphological features and pathogenicity tests. Phylogenetic relationships among 35 isolates were determined based on a data set of internal transcribed spacer (ITS), histone H3 (HIS3) and elongation factor 1‐α (TEF) sequences. In phylogenetic tree analyses, using a combined ITS and TEF sequence alignment, all E. ampelina isolates were clustered together in a single well‐supported clade. In contrast to the absence of genetic variability within ITS and TEF sequences, HIS3 sequences showed 54 polymorphic sites. The haplotype network generated from HIS3 data set showed four distinct haplotypes. EA1 was the predominant haplotype including 29 isolates from both countries. High genetic variability was observed in two Brazilian isolates, haplotype EA4, which may have lost the intron region during species evolution. Colony colours differed between Brazilian and Australian isolates, but showed similar wrinkled colony texture, absence of spores, sparse‐to‐absent white aerial mycelium and slow growth (0.049–0.060 mm/day). Brazilian isolates produced conidia of 5.65 × 2.65 μm, larger than conidia from Australian isolates, which measured 5.14 × 2.30 μm. In pathogenicity tests, all nine Australian isolates inoculated were pathogenic on detached canes and potted vines of table grape.