bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multimodal optical analysis of meningioma and comparison with histopathology
Authors: Zanello Marc , Poulon Fanny , Varlet Pascale , Chretien Fabrice , Andreiuolo Felipe , Pages Mélanie , Ibrahim Ali , Pallud Johan , Dezamis Edouard , Abi‐Lahoud Georges , Nataf François , Turak Baris , Devaux Bertrand , Abi Haidar Darine 
Published: February 2016

Meningioma is the most frequent primary central nervous system tumor. The risk of recurrence and the prognosis are correlated with the extent of the resection that ideally encompasses the infiltrated dura mater and, if required, the infiltrated bone. No device can deliver real‐time intraoperative histopathological information on the tumor environment to help the neurosurgeon to achieve a gross total removal. This study assessed the abilities of nonlinear microscopy to provide relevant and real‐time data to help resection of meningiomas. Nine human meningioma samples (four World Health Organization Grade I, five Grade II) were analyzed using different optical modalities: spectral analysis and imaging, lifetime measurements, fluorescence lifetime imaging microscopy, fluorescence emitted under one‐ and two‐photon excitation and the second‐harmonic generation signal imaging using a multimodal setup. Nonlinear microscopy produced images close to histopathology as a gold standard. The second‐harmonic generation signal delineated the collagen background and two‐photon fluorescence underlined cell cytoplasm. The matching between fluorescence images and Hematoxylin and Eosin staining was possible in all cases. Grade I meningioma emitted less autofluorescence than Grade II meningioma and Grade II meningioma exhibited a distinct lifetime value. Autofluorescence was correlated with the proliferation rates and seemed to explain the observed differences between Grade I and II meningiomas. This preliminary multimodal study focused on human meningioma samples confirms the potential of tissue autofluorescence analysis and nonlinear microscopy in helping intraoperatively neurosurgeons to reach the actual boundaries of the tumor infiltration.
Correspondence between H&E staining (top pictures) and the two‐photon fluorescence imaging (bottom pictures)magnified imageCorrespondence between H&E staining (top pictures) and the two‐photon fluorescence imaging (bottom pictures)
Kinetic analysis of nanoparticle‐protein interactions using a plasmon waveguide resonance
Authors: Bahrami Farshid , Maisonneuve Mathieu , Meunier Michel , Montazeri Arthur O. , Kim Yujin , Kherani Nazir P. , Aitchison J. Stewart , Mojahedi Mo 
Published: February 2016

A plasmon waveguide resonance (PWR) sensor is proposed for studying the interaction between gold nanoparticles and proteins. The ability of the PWR sensor to operate in both TM and TE Polarizations, i.e. its polarization diversity, facilitates the simultaneous spectroscopy of the nanoparticles surface reactions using both polarizations. The response of each polarization to streptavidin‐biotin binding at the surface of gold nanoparticles is investigated in real time. Finally, using the principles of multimode spectroscopy, the nanoparticle's surface reactions are decoupled from the bulk solution refractive index variations.
Schematic diagram of the NP‐modified PWR sensormagnified imageSchematic diagram of the NP‐modified PWR sensor
Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source
Authors: Lefort Claire , O'Connor Rodney P. , Blanquet Véronique , Magnol Laetitia , Kano Hideaki , Tombelaine Vincent , Lévêque Philippe , Couderc Vincent , Leproux Philippe 
Published: February 2016

Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation of 300 nm (full width half maximum) thanks to the implementation a nanosecond supercontinuum (SC) source compact and simple with a low repetition rate. The interest of such a wide spectral bandwidth, never demonstrated until now, is highlighted in vivo: images of glioma tumor cells stably expressing eGFP grafted on the brain of a mouse and its blood vessels network labelled with Texas Red® are obtained. These two fluorophores have a spectral bandwidth covering the whole 300 nm available. In parallel, a similar image quality is obtained on a sample of mouse muscle in vitro when excited with this nanosecond SC source or with a classical high rate, femtosecond and quasi monochromatic laser. This opens the way for (i) a simple and very complete biological characterization never performed to date with multiphoton processes, (ii) multiple means of contrast in nonlinear imaging allowed by the use of numerous fluorophores and (iii) other multiphoton processes like three‐photon ones.
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Fully unsupervised inter‐individual IR spectral histology of paraffinized tissue sections of normal colon
Authors: Nguyen Thi Nguyet Que , Jeannesson Pierre , Groh Audrey , Piot Olivier , Guenot Dominique , Gobinet Cyril 
Published: February 2016

In label‐free Fourier‐transform infrared histology, spectral images are individually recorded from tissue sections, pre‐processed and clustered. Each single resulting color‐coded image is annotated by a pathologist to obtain the best possible match with tissue structures revealed after Hematoxylin‐Eosin staining. However, the main limitations of this approach are the empirical choice of the number of clusters in unsupervised classification, and the marked color heterogeneity between the clustered spectral images. Here, using normal murine and human colon tissues, we developed an automatic multi‐image spectral histology to simultaneously analyze a set of spectral images (8 images mice samples and 72 images human ones). This procedure consisted of a joint Extended Multiplicative Signal Correction (EMSC) to numerically deparaffinize the tissue sections, followed by an automated joint K‐Means (KM) clustering using the hierarchical double application of Pakhira‐Bandyopadhyay‐Maulik (PBM) validity index. Using this procedure, the main murine and human colon histological structures were correctly identified at both the intra‐ and the inter‐individual levels, especially the crypts, secreted mucus, lamina propria and submucosa. Here, we show that batched multi‐image spectral histology procedure is insensitive to the reference spectrum but highly sensitive to the paraffin model of joint EMSC. In conclusion, combining joint EMSC and joint KM clustering by double PBM application allows to achieve objective and automated batched multi‐image spectral histology.
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Sequential culture on biomimetic nanoclay scaffolds forms three‐dimensional tumoroids
Authors: Katti Kalpana S. , Molla MD Shahjahan , Karandish Fataneh , Haldar Manas K. , Mallik Sanku , Katti Dinesh R. 
Published: February 2016

AbstractIn recent times, the limitation of two‐dimensional cultures and complexity ofin vivomodels has paved the way for the development of three‐dimensional models for studying cancer. Here we report the development of a new tumor model using PCL/HAPClay scaffolds seeded with a sequential culture of human mesenchymal stem cells (hMSCs) followed by human prostate cancer cells (HPCCs). This nanocomposite system is used as a test‐bed for studying cancer metastasis and efficacy of anti‐cancer drugs using a polymersome delivery method. A novel sequential cell culture system in three‐dimensionalin vitrobone model provides a unique bone mimetic environment. The hMSCs seeded scaffolds are seeded with prostate cancer cells after the hMSCs have differentiated into osteoblasts. Sequential culture on the scaffolds has shown formation of tumoroids or microtissue consisting of organized, densely packed round cells with hypoxic core regions similar toin vivotumors. Such tumoroids are not observed on HPCC seeded scaffolds or when HPCCs sequentially cultured with human osteoblast cells. Clearly, the newly differentiated hMSCs play a vital role in the ability of cancer cells to grow into tumoroids and cause disease. The PCL/HAPclay scaffold system seeded with the sequential culture of hMSCs, and HPCCs presents a good model system for study of the interactions between prostate cancer cells and bone microenvironment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1591–1602, 2016.
Distinct Patterns of Colocalization of the CCND1 and CMYC Genes With Their Potential Translocation Partner IGH at Successive Stages of B‐Cell Differentiation
Authors: Sklyar Ilya , Iarovaia Olga V. , Gavrilov Alexey A. , Pichugin Andrey , Germini Diego , Tsfasman Tatiana , Caron Gersende , Fest Thierry , Lipinski Marc , Razin Sergey V. , Vassetzky Yegor S. 
Published: February 2016

ABSTRACTThe immunoglobulin heavy chain (IGH) locus is submitted to intra‐chromosomal DNA breakages and rearrangements during normal B cell differentiation that create a risk for illegitimate inter‐chromosomal translocations leading to a variety of B‐cell malignancies. In most Burkitt's and Mantle Cell lymphomas, specific chromosomal translocations juxtapose the IGH locus with a CMYC or Cyclin D1 (CCND1) gene, respectively. 3D‐fluorescence in situ hybridization was performed on normal peripheral B lymphocytes induced to mature in vitro from a naive state to the stage where they undergo somatic hypermutation (SHM) and class switch recombination (CSR). The CCND1 genes were found very close to the IGH locus in naive B cells and further away after maturation. In contrast, the CMYC alleles became localized closer to an IGH locus at the stage of SHM/CSR. The colocalization observed between the two oncogenes and the IGH locus at successive stages of B‐cell differentiation occurred in the immediate vicinity of the nucleolus, consistent with the known localization of the RAGs and AID enzymes whose function has been demonstrated in IGH physiological rearrangements. We propose that the chromosomal events leading to Mantle Cell lymphoma and Burkitt's lymphoma are favored by the colocalization of CCND1 and CMYC with IGH at the time the concerned B cells undergo VDJ recombination or SHM/CSR, respectively. J. Cell. Biochem. 117: 1506–1510, 2016. © 2016 Wiley Periodicals, Inc.
The Effect of a High‐Fat Meal on the Pharmacokinetics of Ixazomib, an Oral Proteasome Inhibitor, in Patients With Advanced Solid Tumors or Lymphoma
Authors: Gupta Neeraj , Hanley Michael J. , Venkatakrishnan Karthik , Wang Bingxia , Sharma Sunil , Bessudo Alberto , Hui Ai‐Min , Nemunaitis John 
Published: February 2016

AbstractIxazomib is the first oral proteasome inhibitor to be investigated in the clinic. This clinical study assessed whether the pharmacokinetics of ixazomib would be altered if administered after a high‐calorie, high‐fat meal. In a 2‐period, 2‐sequence, crossover study design, adult patients with advanced solid tumors or lymphoma received a 4‐mg oral dose of ixazomib as immediate‐release capsules on day 1 without food (fasted, administered following an overnight fast) or with food (fed, following consumption of a high‐calorie, high‐fat meal), followed by another dose on day 15 in the alternate food intake condition (fasted to fed or fed to fasted). Twenty‐four patients were enrolled; of these, 15 were included in the pharmacokinetic‐evaluable population. Administration of ixazomib after a high‐fat meal reduced both the rate and extent of absorption of ixazomib. Under fed conditions, the median time to peak plasma concentration (Tmax) of ixazomib was delayed by approximately 3 hours compared with administration in the fasted state (1.02 hours vs 4.0 hours), and there was a 28% reduction in total systemic exposure (area under the curve, AUC) and a 69% reduction in peak plasma concentration (Cmax). Together, the results support the administration of ixazomib on an empty stomach, at least 1 hour before or at least 2 hours after food. These recommendations are reflected in the United States Prescribing Information for ixazomib (clinicaltrials.gov identifier NCT01454076).
A Critical Evaluation of Pharmacogenetic Information in Package Inserts for Selected Drugs Marketed in India and Its Comparison With US FDA‐Approved Package Inserts
Authors: Pai Sarayu Arvind , Kshirsagar Nilima 
Published: February 2016

AbstractOur objective was to compare the pharmacogenetic information provided in the package inserts (PIs) of 7 drugs marketed in the United States and India, namely, abacavir, capecitabine, carbamazepine, clopidogrel, irinotecan, valproic acid, and warfarin. We evaluated the pharmacogenetic information provided in Indian PIs for the highest level where it was included, robustness and completeness, clinical validity, and clinical utility and compared it with corresponding data of US PIs. Pharmacogenetic studies carried out in India were identified using PubMed. Pharmacogenetic information was provided in Indian PIs of all the drugs except irinotecan. It appeared in the same section as in US PIs for abacavir, capecitabine, carbamazepine (HLA‐*3101), valproic acid (urea cycle disorders), and warfarin (protein C and protein S), whereas it appeared at lower levels for other drug‐gene combinations. The robustness of pharmacogenetic testing was graded convincing for abacavir, adequate for carbamazepine and clopidogrel, and incomplete for the remaining drugs, and only abacavir and clopidogrel PIs provided full details of supporting studies. These details, when provided in the Indian PIs were identical to those in the US PIs. The Indian PIs did not provide data on Indian patients, although published studies are available. Both US and Indian PIs lacked critical information on the clinical validity and utility of pharmacogenetic testing. The pharmacogenetic information should provide country/ethnicity‐specific data so that they are useful to clinicians. Where data are not available, the prevalence of genetic variation in the population of a country needs to be determined and should then be translated to the PIs.
Microtensile strength of resin cement bond to indirect composite treated by different output powers of Er:YAG laser
Authors: Garshasbzadeh Nazanin Zeinab , Mirzaie Mansoreh , Yassini Esmaeil , Shahabi Sima , Benedicenti Stefano , Angiero Francesca , Chiniforush Nasim 
Published: February 2016

ABSTRACTThe study aimed to evaluate the effect of different output powers of Er:YAG laser on microtensile bonding strength of indirect composite to resin cements.36 indirect composite blocks (GC Gradia DA2, Japan) size 15 × 10 × 10 mm3 were constructed, and divided into 12 groups, as follows:G1: control group (no treatment); Groups G2 to G6: treated with Er:YAG laser (2,940 nm) in noncontact mode, frequency 20 Hz, pulse duration 470 µs, with output power ranging from 2W to 6W; Groups G7 sandblasting, Groups 8 to G12: as Groups G2 to G 6 with preparatory sandblasting. One specimen from each group was analyzed by SEM; each specimen was fixed to a specialized metal jig using cyanoacrylate (Mitreapel, Beta Kimya San. Ve TIC, Iran) and debonded under tension with a universal testing machine (Zwick, Germany) at a crosshead speed of 0.5 mm min−1. Sandblasting and laser can improve bond strength above an energy level of 150 mJ. SEM evaluation of laser‐treated specimens showed irregularities and deep undercuts. T test analysis showed no significant difference between sandblasted and non‐sandblasted group, with laser output power of 0, 100, or 150 mJ (P = 0.666, P = 0.875, and P = 0.069); in the specimens irradiated with energy output of 200, 250, or 300 mJ, sandblasted specimens showed higher bond strength than non‐sandblasted ones. The results demonstrate that, in composite resin irradiated with laser at energy output of 200–300 mJ, sandblasting might be a suitable procedure to enhance bond strength of resin cement. Microsc. Res. Tech. 79:328–333, 2016. © 2016 Wiley Periodicals, Inc.
From lizard body form to serpentiform morphology: The atlas–axis complex in African cordyliformes and their relatives
Authors: Čerňanský Andrej 
Published: February 2016

ABSTRACTThe comparative vertebral morphology of the atlas–axis complex in cordyliforms, xantusiid and several skinks is studied here. These lizards are particularly interesting because of their different ecological adaptations and anti‐predation strategies, where conformation ranges from the lizard‐like body to a snake‐like body. This transition to serpentiform morphology shows several evolutionary patterns in the atlas–axis complex: 1) the zygapophyseal articulations are lost in the early stage of the transition. In contrast to mammals, the atlas is more or less locked to the axis in lepidosaurs, but the absence of zygapophyseal articulation releases this locking for rotation. However despite its serpentiform morphology, Chamaesaura is different, in possessing this articulation; 2) the first intercentrum of Chamaesaura and Tetradactylus africanus (serpentiform grass‐swimmers) is fully curved anteriorly, underlying the occipital condyle. While this limits ventral skull rotation beyond a certain angle, it locks the skull, which is a crucial adaptation for a sit‐and‐wait position in grassland habitats that needs to keep the head stabilized; and 3) in Acontias, most of the atlas articular surface with the occipital condyle is formed by the lateral aspect of the articulation area relative to the area located in the dorsal region of the slightly reduced intercentrum. A similar state occurs in amphisbaenians, most likely reflecting a fossorial lifestyle of the limbless lizards.Although Chamaesaura and Tetradactylus live sympatrically in grasslands, Chamaesaura differs in several ways in atlas–axis complex: for example, aforementioned presence of the atlas–axis zygapophyseal articulation, and long posterodorsal processes. Its occipital condyle protrudes further posteriorly, placing the atlas–axis complex further from the endocranium than in Tetradactylus. Hence, adaptation in the same niche, even among sister clades, can lead to different atlas–axis morphology due to different lifestyle strategies, for example, different foraging mode, while similar atlas–axis morphology can evolve in two lineages occupying different niches, as in Ablepharus and Scelotes. J. Morphol. 277:512–536, 2016. © 2016 Wiley Periodicals, Inc.
and T1 assessment of abdominal tissue response to graded hypoxia and hypercapnia using a controlled gas mixing circuit for small animals
Authors: Ganesh Tameshwar , Estrada Marvin , Duffin James , Cheng Hai Ling 
Published: February 2016

PurposeTo characterize
and T1 relaxation time response to a wide spectrum of gas challenges in extracranial tissues of healthy rats.Materials and MethodsA range of graded gas mixtures (hyperoxia, hypercapnia, hypoxia, and hypercapnic hypoxia) were delivered through a controlled gas‐mixing circuit to mechanically ventilated and intubated rats. Quantitative magnetic resonance imaging (MRI) was performed on a 3T clinical scanner;
and T1 maps were computed to determine tissue response in the liver, kidney cortex, and paraspinal muscles. Heart rate and blood oxygen saturation (SaO2) were measured through a rodent oximeter and physiological monitor.Results
decreases consistent with lowered SaO2 measurements were observed for hypercapnia and hypoxia, but decreases were significant only in liver and kidney cortex (P < 0.05) for >10% CO2 and <15% O2, with the new gas stimulus, hypercapnic hypoxia, producing the greatest
decrease. Hyperoxia‐related
increases were accompanied by negligible increases in SaO2. T1 generally increased, if at all, in the liver and decreased in the kidney. Significance was observed (P < 0.05) only in kidney for >90% O2 and >5% CO2.Conclusion
and T1 provide complementary roles for evaluating extracranial tissue response to a broad range of gas challenges. Based on both measured and known physiological responses, our results are consistent with
as a sensitive marker of blood oxygen saturation and T1 as a weak marker of blood volume changes. J. Magn. Reson. Imaging 2016;44:305–316.
Value of preoperative 3T multiparametric MRI for surgical margin status in patients with prostate cancer
Authors: Tamada Tsutomu , Sone Teruki , Kanomata Naoki , Miyaji Yoshiyuki , Kido Ayumu , Jo Yoshimasa , Yamamoto Akira , Ito Katsuyoshi 
Published: February 2016

PurposeTo examine the value of preoperative multiparametric magnetic resonance imaging (MRI) as a predictor of surgical margin (SM) status in patients with prostate cancer (PC).Materials and MethodsThe Institutional Review Board approved this retrospective study; the requirement for informed consent was waived. Fifty‐six male patients with histologically proven PC underwent preoperative 3T multiparametric MRI including high b value (0, 2000 s/mm2) diffusion‐weighted imaging. In each patient, clinical data, such as biopsy Gleason score and D'Amico clinical risk score, and multiparametric MRI findings, such as tumor location, tumor size, tumor extension in the apical or proximal region, tumor apparent diffusion coefficient (ADC), and the presence or absence of MRI findings of extracapsular extension (ECE) were evaluated. Statistical evaluations included the Fisher's exact test, χ2 test, Mann–Whitney U‐test, and logistic regression analysis.ResultsOn histopathological evaluation, 15 patients (27%) were SM‐positive (SMP group), and 41 (73%) were SM‐negative (SMN group). The tumor ADC was significantly lower in the SMP group than in the SMN group (P = 0.001). The frequency of tumor extension in the apex or base and suspected ECE on MRI were significantly higher in the SMP group than in the SMN group (P = 0.037 and 0.011, respectively). On multivariate analysis, tumor ADC was the only predictor of SM status in PC (P = 0.003).ConclusionPC with positive SM was characterized by tumor extension in the apical and proximal regions, lower tumor ADC, and tumors with positive MRI findings of ECE, compared to tumors with negative SM. J. Magn. Reson. Imaging 2016;44:584–593.
Age‐related optimization of screw placement for reduced loosening risk in locked plating
Authors: MacLeod Alisdair R. , Simpson A. Hamish R. W. , Pankaj Pankaj 
Published: February 2016

ABSTRACTWhen using locked plating for bone fracture fixation, screw loosening is reported as one of the most frequent complications and is commonly attributed to an incorrect choice of screw configuration. Choosing a patient‐optimized screw configuration is not straightforward as there are many interdependent variables that affect device performance. The aim of the study was to evaluate the influence that locking screw configuration has on loosening risk and how this is influenced by bone quality. This study uses finite element models that incorporate cortical bone heterogeneity, orthotropy, and geometrical nonlinearity to examine the effect of screw configuration on variables associated with loosening and interfragmentary motion. Strain levels within the bone were used as indicators of regions that may undergo loosening. The study found that, in healthy bone under axial loading, the most important variables influencing strain levels within the bone were the size of the bridging span (working length) and the plate rigidity. Unlike healthy bone, osteoporotic bone was found to be particularly sensitive to the spacing of the screws within the plate. Using two empty screw holes between the screws closest to the fracture was found to reduce the strain levels at the first screw by 49% in osteoporotic bone (compared to only 2.4% in healthy bone). The study also found that under torsional loading the total number of screws used was the most important variable with a 59% reduction in the strain around the screws closest to the fracture when using six rather than four screws in osteoporotic bone. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1856–1864, 2016.