bluuebunny/crossref_metadata_2025_split huggingface dataset:
Finished Whole-Genome Sequences of Two Clostridium botulinum Type A(B) Isolates
Authors: Halpin Jessica L. , Hill Karen , Johnson Shannon L. , Bruce David Carlton , Shirey T. Brian , Dykes Janet K. , Lúquez Carolina 
Published: May 2017

ABSTRACT

Clostridium botulinum
secretes a potent neurotoxin that causes devastating effects when ingested, including paralysis and death if not treated. In the United States, some clinically significant strains produce toxin type A while also harboring a silent B gene. These are the first two closed genome sequences published for this subset.
Complete Genome Sequence of Moraxella catarrhalis Strain CCRI-195ME, Isolated from the Middle Ear
Authors: Tan Aimee , Blakeway Luke V. , Bakaletz Lauren O. , Boitano Matthew , Clark Tyson A. , Korlach Jonas , Jennings Michael P. , Peak Ian R. , Seib Kate L. 
Published: May 2017

ABSTRACT

Moraxella catarrhalis
is an important bacterial pathogen that causes otitis media and exacerbations of chronic obstructive pulmonary disease. Here, we report the complete genome sequence of
M. catarrhalis
strain CCRI-195ME, which contains the phase-variable epigenetic regulator ModM3.
Draft Genome Sequence of the Salt Water Bacterium Oceanospirillum linum ATCC 11336
T
Authors: Trachtenberg Ariel M. , Carney Joshua G. , Linnane Joshua D. , Rheaume Bruce A. , Pitts Natalie L. , Mykles Donald L. , MacLea Kyle S. 
Published: May 2017

ABSTRACT

Oceanospirillum linum
ATCC 11336
T
is an aerobic, bipolar-tufted gammaproteobacterium first isolated in the Long Island Sound in the 1950s. This announcement offers a genome sequence for
O. linum
ATCC 11336
T
, which has a predicted genome size of 3,782,189 bp (49.13% G+C content) containing 3,540 genes and 3,361 coding sequences.
Draft Genome Sequence of Microbacterium foliorum Strain 122 Isolated from a Plant Growing in a Chronically Hydrocarbon-Contaminated Site
Authors: Lumactud Rhea , Fulthorpe Roberta , Sentchilo Vladimir , van der Meer Jan Roelof 
Published: May 2017

ABSTRACT

Microbacterium foliorum
strain 122 is a bacterial endophyte isolated from a
Dactylis glomerata
plant growing in a natural oil seep soil located in Oil Springs, Ontario, Canada. We present here a draft genome sequence of an endophytic strain that has promising potential in hydrocarbon degradation and plant growth promotion.
Progression of lamellar hole-associated epiretinal proliferation and retinal changes during long-term follow-up
Authors: Compera Denise , Schumann Ricarda G , Cereda Matteo G , Acquistapace Alessandra , Lita Viviane , Priglinger Siegfried G , Staurenghi Giovanni , Bottoni Ferdinando 
Published: May 2017

PurposeTo report on progression of lamellar hole-associated epiretinal proliferation (LHEP) in eyes with lamellar macular holes (LMH) using spectral-domain optical coherence tomography (SD-OCT), and to correlate with intraretinal changes and visual function.MethodsFrom a retrospectively reviewed series of 167 eyes with non-full-thickness macular holes, we exclusively included a subgroup of 34 eyes with LMH and LHEP by SD-OCT evaluation. In these eyes, area of LHEP, intraretinal changes of defect diameter, central retinal thickness, defects of the ellipsoid zone and occurrence of a contractive epiretinal membrane were analysed. Additionally, clinical data were documented.ResultsArea of LHEP significantly increased during a mean follow-up period of 40.5 months (median 52 months). Analysing intraretinal changes, a significant enlargement of minimum and maximum horizontal lamellar hole diameter was found that correlated with the area of LHEP. Defects of the ellipsoid zone were seen in 65% of the eyes at baseline and in 85% at the end of follow-up. Increase of maximum horizontal hole diameter and ellipsoid zone defects correlated with a decline of visual acuity. Fifty per cent of patients with LMH and LHEP also demonstrated extrafoveal typical contractive epiretinal membranes with retinal folds.ConclusionsLong-term follow-up revealed an increase of the area of LHEP in eyes with LMH that correlated with the enlargement of lamellar hole diameter and ellipsoid zone defects. Our data delineate the progression of intraretinal changes in association with a decline of visual function in this subgroup of LMH eyes.
The effects of welded joint characteristics on its properties in HDPE thermal fusion welding
Authors: Dai Hongbin , Peng Jun 
Published: May 2017

In this paper, PE100 pipes with the diameter of 200 mm and the thickness of 11.9 mm were used as material. The welded joints were obtained in different welding pressures with the optimal welding temperature of 220[Formula: see text]C. Reheating process on the welded joints with the temperature of 130[Formula: see text]C was carried out. The joints exhibited X-type, and the cause of X-type joints was discussed. The temperature field in the forming process of welded joints was measured, and tensile and bending tests on welded joints were carried out. The fracture surface of welded joints was observed by scanning electron microscopy (SEM), and crystallinity calculation was taken by X-ray diffraction (XRD). The mechanism of X-type weld profile effects on welded joints properties was analyzed. It was concluded that the mechanical properties of welded joints decrease with the reduced X distance between lines.
Estimating friction in normal fault systems of the Basin and Range province and examining its geological context
Authors: Richardson Carson A. , Seedorff Eric 
Published: May 2017

Abstract

The life cycle of a fault following initiation is governed in part by the reshear criterion, of which rock surface friction is the critical factor limiting the dip of a fault at its death. Using structural restorations where the initial and final dips of faults can be ascertained, the coefficient of rock surface friction is calculated for well-characterized extended locales (
n
=20) in the Basin and Range province, many with multiple fault generations (
n
=34). The calculated values exhibit a considerably wider range (0.19–1.33) than previously reported. The amount of tilting associated with each fault generation is compared with eight characteristics (mean slip magnitude, tilting per unit of slip, fault spacing, percentage extension, absence or presence and composition of magmatism, duration of extension, timing of extension and strain rate). No statistically strong correlation was found with any of the examined characteristics, although tentative linkages were noted with percentage extension, strain rate and mean slip magnitude from weighted regression analysis. These results are consistent with normal faults behaving as non-linear systems, with friction being an emergent property.
Antarctic subglacial groundwater: a concept paper on its measurement and potential influence on ice flow
Authors: Siegert Martin J. , Kulessa Bernd , Bougamont Marion , Christoffersen Poul , Key Kerry , Andersen Kristoffer R. , Booth Adam D. , Smith Andrew M. 
Published: May 2017

Abstract
Is groundwater abundant in Antarctica and does it modulate ice flow? Answering this question matters because ice streams flow by gliding over a wet substrate of till. Water fed to ice-stream beds thus influences ice-sheet dynamics and, potentially, sea-level rise. It is recognized that both till and the sedimentary basins from which it originates are porous and could host a reservoir of mobile groundwater that interacts with the subglacial interfacial system. According to recent numerical modelling, up to half of all water available for basal lubrication, and time lags between hydrological forcing and ice-sheet response as long as millennia, may have been overlooked in models of ice flow. Here, we review evidence in support of Antarctic groundwater and propose how it can be measured to ascertain the extent to which it modulates ice flow. We present new seismoelectric soundings of subglacial till, and magnetotelluric and transient electromagnetic forward models of subglacial groundwater reservoirs. We demonstrate that multifaceted and integrated geophysical datasets can detect, delineate and quantify the groundwater contents of subglacial sedimentary basins and, potentially, monitor groundwater exchange rates between subglacial till layers. The paper thus describes a new area of glaciological investigation and how it should progress in future.
Experimental cardiac radiation exposure induces ventricular diastolic dysfunction with preserved ejection fraction
Authors: Saiki Hirofumi , Moulay Gilles , Guenzel Adam J. , Liu Weibin , Decklever Teresa D. , Classic Kelly L. , Pham Linh , Chen Horng H. , Burnett John C. , Russell Stephen J. , Redfield Margaret M. 
Published: May 2017

Breast cancer radiotherapy increases the risk of heart failure with preserved ejection fraction (HFpEF). Cardiomyocytes are highly radioresistant, but radiation specifically affects coronary microvascular endothelial cells, with subsequent microvascular inflammation and rarefaction. The effects of radiation on left ventricular (LV) diastolic function are poorly characterized. We hypothesized that cardiac radiation exposure may result in diastolic dysfunction without reduced EF. Global cardiac expression of the sodium-iodide symporter (NIS) was induced by cardiotropic gene (adeno-associated virus serotype 9) delivery to 5-wk-old rats. SPECT/CT (125I) measurement of cardiac iodine uptake allowed calculation of the 131I doses needed to deliver 10- or 20-Gy cardiac radiation at 10 wk of age. Radiated (Rad; 10 or 20 Gy) and control rats were studied at 30 wk of age. Body weight, blood pressure, and heart rate were similar in control and Rad rats. Compared with control rats, Rad rats had impaired exercise capacity, increased LV diastolic stiffness, impaired LV relaxation, and elevated filling pressures but similar LV volume, EF, end-systolic elastance, preload recruitable stroke work, and peak +dP/d t. Pathology revealed reduced microvascular density, mild concentric cardiomyocyte hypertrophy, and increased LV fibrosis in Rad rats compared with control rats. In the Rad myocardium, oxidative stress was increased and in vivo PKG activity was decreased. Experimental cardiac radiation exposure resulted in diastolic dysfunction without reduced EF. These data provide insight into the association between cardiac radiation exposure and HFpEF risk and lend further support for the importance of inflammation-related coronary microvascular compromise in HFpEF. NEW & NOTEWORTHY Cardiac radiation exposure during radiotherapy increases the risk of heart failure with preserved ejection fraction. In a novel rodent model, cardiac radiation exposure resulted in coronary microvascular rarefaction, oxidative stress, impaired PKG signaling, myocardial fibrosis, mild cardiomyocyte hypertrophy, left ventricular diastolic dysfunction, and elevated left ventricular filling pressures despite preserved ejection fraction.
Mechanical behavior and matrisome gene expression in the aneurysm-prone thoracic aorta of newborn lysyl oxidase knockout mice
Authors: Staiculescu Marius Catalin , Kim Jungsil , Mecham Robert P. , Wagenseil Jessica E. 
Published: May 2017

Mutations in lysyl oxidase (LOX) are associated with thoracic aortic aneurysm and dissection (TAAD). Mice that do not express Lox ( Lox−/−) die soon after birth and have 60% and 40% reductions in elastin- and collagen-specific cross-links, respectively. LOX inactivation could also change the expression of secreted factors, the structural matrix, and matrix-associated proteins that constitute the aortic matrisome. We hypothesized that absence of Lox will change the mechanical behavior of the aortic wall because of reduced elastin and collagen cross-linking and alter the expression levels of matrisome and smooth muscle cell (SMC) genes in a vascular location-specific manner. Using fluorescence microscopy, pressure myography, and gene set enrichment analysis, we visualized the microarchitecture, quantified the mechanical behavior, and examined matrisome and SMC gene expression from ascending aortas (AAs) and descending aortas (DAs) from newborn Lox+/+ and Lox−/− mice. Even though Lox−/− AAs and DAs have fragmented elastic laminae and disorganized SMCs, the unloaded outer diameter and wall thickness were similar to Lox+/+ AAs and DAs. Lox−/− AAs and DAs have altered opening angles, circumferential stresses, and circumferential stretch ratios; however, only Lox−/− AAs have increased pressurized diameters and tangent moduli. Gene set enrichment analysis showed upregulation of the extracellular matrix (ECM) regulator gene set in Lox−/− AAs and DAs as well as differential expression of secreted factors, collagens, ECM-affiliated proteins, ECM glycoproteins, and SMC cell cycle gene sets that depend on the Lox genotype and vascular location. These results provide insights into the local chemomechanical changes induced by Lox inactivation that may be important for TAAD pathogenesis. NEW & NOTEWORTHY Absence of lysyl oxidase ( Lox) causes thoracic aortic aneurysms. The aortic mechanical behavior of Lox−/− mice is consistent with reduced elastin and collagen cross-linking but demonstrates vascular location-specific differences. Lox−/− aortas show upregulation of matrix remodeling genes and location-specific differential expression of other matrix and smooth muscle cell gene sets.
<b>A new species of sea squirt <I>Rhopalaea bilobata</I> (Ascidiacea: Diazonidae) from the Andaman Islands, India</b>
Authors: Mondal Jhimli , Raghunathan C. , Mondal Tamal 
Published: May 2017

Rhopalaea bilobata, a new species of the class Ascidiacea, under the family Diazonidae has been described from the Andaman Islands of Andaman & Nicobar, India. The species was found in sandy bottoms, reef areas and artificial structures at a depth range of 10–40 m. This species has bilobed anal border, six lobed branchial and atrial siphons, transparent thorax with pigmented spots at the anus, between the two siphons and at the anterior end of endostyle, and ramified basal test. The species is closely related to R. idoneta, R. macrothorax and R. tenuis in several sets of anatomical and morphological features.
<b>Butterfly fauna of Baghmundi, Purulia, West Bengal, India: a preliminary checklist</b>
Authors: Samanta Supriya , Das Dipanwita , Mandal Sudipta 
Published: May 2017

The butterfly diversity of Baghmundi, Purulia District (22.600–23.500N & 85.750–86.650E), West Bengal, India was studied from January 2014 to December 2015 with photographic documentation. A total of 54 butterfly species were recorded representing 39 genera in six families. Nymphalidae was the most dominant family with 46.3% of the total species. As this is the first report of butterfly diversity from this region, the present study may help in planning conservation strategies and generate awareness among the local people and government authorities to save wildlife and their habitats.
<b>Stomach contents of the Indian Pangolin <I>Manis crassicaudata</I> (Mammalia: Pholidota: Manidae) in tropical forests of southern India</b>
Authors: Ashokkumar Mohanarangan , Valsarajan Dipika , Suresh M. Arjun , Kaimal Anuraj R. , Chandy George 
Published: May 2017

The Indian Pangolin, Manis crassicaudata is a nocturnal, elusive, sparsely distributed, poorly studied and rapidly declining species. The stomach contents of a female Indian Pangolin were analysed from a road kill specimen collected from the South Wayanad Forest Division in Kerala on September 2013 and confirms that they are myrmecophagous, i.e., feeding primarily on ants and ant eggs. The morphometry of the head and position of the eye in the head was used to identify the ant species consumed, which was found to be exclusively Leptogenys sp. with head size of one to two millimeter. Since the abundance of the ant species was not available, the preference could not be determined. Rather, our preliminary survey in the area revealed that Leptogenys sp. is the most abundant in the tropical moist deciduous forest of Wayanad, Kerala. The soft parts of the ant body were digested while only the heads remained in the stomach content. Similarly, only hard egg shells could be identified from the content. The major proportion of the stomach content was grit (50%), both in frequency and biomass. Ant head constituted higher biomass (41.3%) and lower frequency (22.5%) whereas ant egg shells were found in higher number (29%) with a low biomass (1.8%). The present observation gives insight into ant species selection and biomass contribution. Though the biomass of ant head was higher, its contribution to the Indian Pangolin nutrient intake is unknown. Further research is needed to better understand the diet of the Indian Pangolin.
<b>Freshwater fish fauna of Hiranyakeshi River, the northern Western Ghats, India</b>
Authors: Kumkar Pradeep , Kharat Sanjay S. , Sawant Nitin S. , Katwate Unmesh , Dahanukar Neelesh 
Published: May 2017

The freshwater fish fauna of Hiranyakeshi River, a tributary of the Krishna River System, originating in the Western Ghats of Maharashtra, was studied for a period of three years from 2013–2016. We reported 57 species belonging to seven orders, 17 families and 42 genera. Cypriniformes was the most species rich order followed by Siluriformes, while Cyprinidae was the most predominant family. At least 21 freshwater fish species found in Hiranyakeshi River are endemic to river systems originating from the Western Ghats, while nine species are endemic to the Krishna River system. The true diversity of freshwater fishes in this region, however, is still obscure because of lack of detailed taxonomic studies in this region. Of the total species, at least 11 are in the threatened and near threatened categories of the IUCN Red List and Hiranyakeshi River holds a good population of most of these species. The fish fauna in this region, however, is threatened by several stressors including invasive fishing practices, water impediment due to upcoming dams, invasive introduced species and water pollution due to agricultural runoffs and industrial effluents.
<b><I>Ixora polyantha</I> Wight (Rubiaceae) a new record for northeastern India</b>
Authors: Narah Deiji , Bhat Nazir Ahmad , Kumar Yogendra 
Published: May 2017

Ixora polyantha Wight earlier known to occur in Western Ghats of India and Vietnam is now recorded for the first time from Assam, northeastern India. Along with taxonomic description, detailed illustration and photographs are provided to facilitate easy identification.
<b>On two little-known terrestrial South Asian geckoes <I>Hemidactylus reticulatus</I> and <I>Hemidactylus scabriceps</I> (Reptilia: Gekkonidae)</b>
Authors: Ganesh S. R. , Rameshwaran M. , Joseph Naveen A. , Jerith Ahamed M. 
Published: May 2017

We studied two poorly known terrestrial geckoes inhabiting peninsular India namely, Hemidactylus reticulatus Beddome, 1870 and H. scabriceps (Annandale, 1906). We present data on their morphometry, scalation, colouration and field observations based on both studies in the field and on voucher specimens. Hemipenal morphology for H. scabriceps is presented herein for the first time. Seen in light of some recent cases of misidentification between these two ecologically-similar parapatric species, the present work aids in precise identification and presents landscape clues that help understand their ecology.
<b>Notes on the extended distribution of two threatened species of <I>Strobilanthes</I> Blume (Acanthaceae) in Kerala, India</b>
Authors: Josekutty E.J. , Biju P. , Augustine Jomy 
Published: May 2017

The extended distributional record of two threatened species of Strobilanthes namely S. canarica and S. campanulata in Kerala is provided. They exhibit narrow range of habitats in Western Ghats with highly restricted distribution. Notes on taxonomy, phenology, distribution and conservation status are also given.
<b>Observations on the association of <I>Scaphula deltae</I> Blanford (Mollusca: Bivalvia) in Garanga Wetland of Pobitora Wildlife Sanctuary, Assam, India</b>
Authors: Kalita Girindra 
Published: May 2017

During a survey of the Garanga wetland of Pobitora Wildlife Sanctuary, Scaphula deltae Blanford (Mollusca: Bivalvia) was recorded for the first time. Most examples were found over Lamellidens corrianus (Lea) in a firmly attested position. Some were also found clinging to the roots of Eichhornia crassipes. These findings establish a new record of freshwater mollusc association in the study area. Some of the physico-chemical parameters of the study area were also investigated.
iNOS-Derived Nitric Oxide Induces Integrin-Linked Kinase Endocytic Lysosome-Mediated Degradation in the Vascular Endothelium
Authors: Reventun Paula , Alique Matilde , Cuadrado Irene , Márquez Susana , Toro Rocío , Zaragoza Carlos , Saura Marta 
Published: May 2017

Objective—
ILK (integrin-linked kinase) plays a key role in controlling vasomotor tone and is decreased in atherosclerosis. The objective of this study is to test whether nitric oxide (NO) regulates ILK in vascular remodeling.

Approach and Results—
We found a striking correlation between increased levels of inducible nitric oxide and decreased ILK levels in human atherosclerosis and in a mouse model of vascular remodeling (carotid artery ligation) comparing with iNOS (inducible NO synthase) knockout mice. iNOS induction produced the same result in mouse aortic endothelial cells, and these effects were mimicked by an NO donor in a time-dependent manner. We found that NO decreased ILK protein stability by promoting the dissociation of the complex ILK/Hsp90 (heat shock protein 90)/eNOS (endothelial NO synthase), leading to eNOS uncoupling. NO also destabilized ILK signaling platform and lead to decreased levels of paxillin and α-parvin. ILK phosphorylation of its downstream target GSK3-β (glycogen synthase kinase 3 beta) was decreased by NO. Mechanistically, NO increased ILK ubiquitination mediated by the E3 ubiquitin ligase CHIP (C terminus of HSC70-interacting protein), but ILK ubiquitination was not followed by proteasome degradation. Alternatively, NO drove ILK to degradation through the endocytic-lysosomal pathway. ILK colocalized with the lysosome marker LAMP-1 (lysosomal-associated membrane protein 1) in endothelial cells, and inhibition of lysosome activity with chloroquine reversed the effect of NO. Likewise, ILK colocalized with the early endosome marker EEA1 (early endosome antigen 1). ILK endocytosis proceeded via dynamin because a specific inhibitor of dynamin (Dyngo 4a) was able to reverse ILK endocytosis and its lysosome degradation.

Conclusions—
Endocytosis regulates ILK signaling in vascular remodeling where there is an overload of inducible NO, and thus its inhibition may represent a novel target to fight atherosclerotic disease.