bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clenbuterol reduces GABAergic transmission in prefrontal cortex layer 5/6 pyramidal neurons of juvenile rat via reducing action potentials firing frequency of GABAergic interneurons
Authors: Luo F. , Zhou H. 
Published: October 2017

Beta‐adrenoceptors (β2‐ARs) have beneficial effects on prefrontal cortex (PFC) working memory, however, the cellular and molecular mechanisms are unclear yet. In this study, we probed the effect of β2‐AR‐selective agonist clenbuterol (Clen) on synaptic transmission in layer 5/6 pyramidal neurons of PFC. Bath application of Clen reduced spontaneous IPSC (sIPSC) frequency without effects on sEPSCs. Clen did not alter the frequency and amplitude of miniature IPSCs (mIPSCs), but exerted heterogeneous effects on evoked IPSCs (eIPSCs) recorded from PFC layer 5/6 pyramidal neurons. Clen decreased the firing rate of action potentials of fast‐spiking GABAergic interneurons. Clen‐induced hyperpolarization of fast‐spiking GABAergic interneurons required potentiation of an inward rectifier K+ channels. Clen‐induced hyperpolarization of fast‐spiking interneurons was dependent on Gs protein rather than cAMP and protein kinase A. Our findings demonstrate that Clen (10 μM) enhances inward rectifier K+ channels via Gs protein to cause membrane hyperpolarization of fast‐spiking GABAergic interneurons resulting in reduction of action potentials firing rate to reduce GABAergic transmission.
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Tooth Color as a Predictor of Oral Health‐Related Quality of Life in Young Adults
Authors: Kovacevic Pavicic Daniela , Pavlic Andrej , Kinkela Devcic Maja , Lajnert Vlatka , Spalj Stjepan 
Published: October 2017

AbstractPurposeSmiling plays an important role in social interaction. The purpose of this research was to explore the extent to which objective parameters of color of one's own teeth affected the social and emotional dimensions of young adults’ lives.Materials and MethodsThe sample included 134 subjects—students of the University of Rijeka, Croatia (65% female) aged 19 to 28 years (median 21). All subjects had six intact maxillary anterior teeth without restorations or severe malocclusions and healthy gingiva with no signs of inflammation. Tooth color was assessed intraorally using a spectrophotometer. Lightness, chroma, and translucency of the right maxillary central incisors (the reference teeth) were calculated and used for analysis. Subjects reported dimensions of their oral health‐related quality of life (OHRQoL) using the Oral Health Impact Profile (OHIP), Orofacial Esthetic Scale (OES), and the Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ). Linear relationships between elements of tooth color and OHRQoL were explored using Pearson correlations. Multiple linear regression, while controlling for the influence of age and gender, was also calculated. ANOVA with a Tukey post hoc test was employed to test whether nonlinear relationships existed between OHRQoL and categories of color elements.ResultsDental self‐confidence, esthetic concerns, orofacial appearance, social impact and psychological impact were not related to lightness, chroma, or translucency of the subjects’ teeth. Neither linear nor nonlinear relationships were detected between those aspects. Satisfaction with smile esthetics was only related to translucency where subjects with moderate translucency were least likely to be satisfied (p = 0.033). Women tended to report greater psychosocial impacts than men (p < 0.05), regardless of their tooth color.ConclusionAccording to the results of this study objective, measurable, quantitative parameters of tooth color did not accurately predict psychosocial dimensions of OHRQoL in dentate young adults.
Geographically widespread honeybee‐gut symbiont subgroups show locally distinct antibiotic‐resistant patterns
Authors: Ludvigsen Jane , Porcellato Davide , L'Abée‐Lund Trine M. , Amdam Gro V. , Rudi Knut 
Published: October 2017

AbstractHow long‐term antibiotic treatment affects host bacterial associations is still largely unknown. The honeybee‐gut microbiota has a simple composition, so we used this gut community to investigate how long‐term antibiotic treatment affects host‐associated microbiota. We investigated the phylogenetic relatedness, genomic content (GC percentage, genome size, number of genes and CRISPR) and antibiotic‐resistant genes (ARG) for strains from two abundant members of the honeybee core gut microbiota (Gilliamella apicola and Snodgrassella alvi). Domesticated honeybees are subjected to geographically different management policies, so we used two research apiaries, representing different antibiotic treatment regimens in their apiculture: low antibiotic usage (Norway) and high antibiotic usage (Arizona, USA). We applied whole‐genome shotgun sequencing on 48 G. apicola and 22 S. alvi. We identified three predominating subgroups of G. apicola in honeybees from both Norway and Arizona. For G. apicola, genetic content substantially varied between subgroups and distance similarity calculations showed similarity discrepancy between subgroups. Functional differences between subgroups, such as pectin‐degrading enzymes (G. apicola), were also identified. In addition, we identified horizontal gene transfer (HGT) of transposon (Tn10)‐associated tetracycline resistance (Tet B) across the G. apicola subgroups in the Arizonan honeybees, using interspace polymorphisms in the Tet B determinant. Our results support that honeybee‐gut symbiont subgroups can resist long‐term antibiotic treatment and maintain functionality through acquisition of geographically distinct antibiotic‐resistant genes by HGT.
Know your farmer: Ancient origins and multiple independent domestications of ambrosia beetle fungal cultivars
Authors: Vanderpool Dan , Bracewell Ryan R. , McCutcheon John P. 
Published: October 2017

AbstractBark and ambrosia beetles are highly specialized weevils (Curculionidae) that have established diverse symbioses with fungi, most often from the order Ophiostomatales (Ascomycota, Sordariomycetes). The two types of beetles are distinguished by their feeding habits and intimacy of interactions with their symbiotic fungi. The tree tissue diet of bark beetles is facilitated by fungi, while ambrosia beetles feed solely on fungi that they farm. The farming life history strategy requires domestication of a fungus, which the beetles consume as their sole food source. Ambrosia beetles in the subfamily Platypodinae originated in the mid‐Cretaceous (119–88 Ma) and are the oldest known group of farming insects. However, attempts to resolve phylogenetic relationships and the timing of domestication events for fungal cultivars have been largely inconclusive. We sequenced the genomes of 12 ambrosia beetle fungal cultivars and bark beetle associates, including the devastating laurel wilt pathogen, Raffaelea lauricola, to estimate a robust phylogeny of the Ophiostomatales. We find evidence for contemporaneous diversification of the beetles and their associated fungi, followed by three independent domestication events of the ambrosia fungi genus Raffaelea. We estimate the first domestication of an Ophiostomatales fungus occurred ~86 Ma, 25 million years earlier than prior estimates and in close agreement with the estimated age of farming in the Platypodinae (96 Ma). Comparisons of the timing of fungal domestication events with the timing of beetle radiations support the hypothesis that the first large beetle radiations may have spread domesticated “ambrosia” fungi to other fungi‐associated beetle groups, perhaps facilitating the evolution of new farming lineages.
Distinct microbial communities in the active and permafrost layers on the Tibetan Plateau
Authors: Chen Yong‐Liang , Deng Ye , Ding Jin‐Zhi , Hu Hang‐Wei , Xu Tian‐Le , Li Fei , Yang Gui‐Biao , Yang Yuan‐He 
Published: October 2017

AbstractPermafrost represents an important understudied genetic resource. Soil microorganisms play important roles in regulating biogeochemical cycles and maintaining ecosystem function. However, our knowledge of patterns and drivers of permafrost microbial communities is limited over broad geographic scales. Using high‐throughput Illumina sequencing, this study compared soil bacterial, archaeal and fungal communities between the active and permafrost layers on the Tibetan Plateau. Our results indicated that microbial alpha diversity was significantly higher in the active layer than in the permafrost layer with the exception of fungal Shannon–Wiener index and Simpson's diversity index, and microbial community structures were significantly different between the two layers. Our results also revealed that environmental factors such as soil fertility (soil organic carbon, dissolved organic carbon and total nitrogen contents) were the primary drivers of the beta diversity of bacterial, archaeal and fungal communities in the active layer. In contrast, environmental variables such as the mean annual precipitation and total phosphorus played dominant roles in driving the microbial beta diversity in the permafrost layer. Spatial distance was important for predicting the bacterial and archaeal beta diversity in both the active and permafrost layers, but not for fungal communities. Collectively, these results demonstrated different driving factors of microbial beta diversity between the active layer and permafrost layer, implying that the drivers of the microbial beta diversity observed in the active layer cannot be used to predict the biogeographic patterns of the microbial beta diversity in the permafrost layer.
Familial social structure and socially driven genetic differentiation in Hawaiian short‐finned pilot whales
Authors: Van Cise Amy M. , Martien Karen. K. , Mahaffy Sabre D. , Baird Robin W. , Webster Daniel L. , Fowler James H. , Oleson Erin M. , Morin Phillip A. 
Published: October 2017

AbstractSocial structure can have a significant impact on divergence and evolution within species, especially in the marine environment, which has few environmental boundaries to dispersal. On the other hand, genetic structure can affect social structure in many species, through an individual preference towards associating with relatives. One social species, the short‐finned pilot whale (Globicephala macrorhynchus), has been shown to live in stable social groups for periods of at least a decade. Using mitochondrial control sequences from 242 individuals and single nucleotide polymorphisms from 106 individuals, we examine population structure among geographic and social groups of short‐finned pilot whales in the Hawaiian Islands, and test for links between social and genetic structure. Our results show that there are at least two geographic populations in the Hawaiian Islands: a Main Hawaiian Islands (MHI) population and a Northwestern Hawaiian Islands/Pelagic population (FSTand ΦSTp < .001), as well as an easternMHIcommunity and a westernMHIcommunity (FSTp = .009). We find genetically driven social structure, or high relatedness among social units and clusters (p < .001), and a positive relationship between relatedness and association between individuals (p < .0001). Further, socially organized clusters are genetically distinct, indicating that social structure drives genetic divergence within the population, likely through restricted mate selection (FSTp = .05). This genetic divergence among social groups can make the species less resilient to anthropogenic or ecological disturbance. Conservation of this species therefore depends on understanding links among social structure, genetic structure and ecological variability within the species.
Divergence of species‐specific protein sex pheromone blends in two related, nonhybridizing newts (Salamandridae)
Authors: Treer Dag , Maex Margo , Van Bocxlaer Ines , Proost Paul , Bossuyt Franky 
Published: October 2017

AbstractIn animals that use chemical communication during courtship and reproduction, speciation is often associated with divergence of their sex pheromones. In multicomponent pheromone systems, divergence can be obtained either by adding or deleting components, or by altering the relative contribution of individual components to the mixture. Protein pheromone systems can additionally evolve by amino acid sequence divergence to produce pheromones with a species‐specific effect. The sodefrin precursor‐like factor (SPF) pheromone system, a blend of proteins that essentially enhances receptivity in salamanders, has had a long and dynamic evolution of gene duplications, but the mechanisms that govern interspecific divergence and the role they play in reproductive isolation remain elusive. Here, we use transcriptomics and proteomics to characterize the SPF protein repertoire of the alpine newt (Ichthyosaura alpestris), and compare it to the previously identified repertoire of SPF proteins of the palmate newt (Lissotriton helveticus), a related but nonhybridizing species. Subsequent phylogenetic analyses indicate that, despite the availability of multiple SPF gene copies, both species predominantly express the same subset of orthologs. Our study demonstrates that species specificity in the SPF protein pheromone system can be established by gradual sequence divergence of the same set of proteins alone.
Breakdown of a defensive symbiosis, but not endogenous defences, at elevated temperatures
Authors: Doremus Matthew R. , Smith Andrew H. , Kim Kyungsun L. , Holder Angela J. , Russell Jacob A. , Oliver Kerry M. 
Published: October 2017

AbstractEnvironmental factors, including temperature, can have large effects on species interactions, including mutualisms and antagonisms. Most insect species are infected with heritable bacterial symbionts with many protecting their hosts from natural enemies. However, many symbionts or their products are thermally sensitive; hence, their effectiveness may vary across a range of temperatures. In the pea aphid, Acyrthosiphon pisum, the bacterial symbiont Hamiltonella defensa and its associated APSE bacteriophages confer resistance to this aphid's dominant parasitoid, Aphidius ervi. Here, we investigate the effects of temperature on both endogenous and symbiont‐based protection against this parasitoid. We also explored the defensive properties of the X‐type symbiont, a bacterium hypothesized to shape aphid defence when co‐occurring with H. defensa. We show that H. defensa protection fails at higher temperatures, although some aphid genotype and H. defensa strain combinations are more robust than others at moderately warmer temperatures. We also found that a single X‐type strain neither defended against parasitism by A. ervi nor rescued lost H. defensa protection at higher temperatures. In contrast, endogenous aphid resistance was effective across temperatures, revealing that these distinct defensive modes are not equally robust to changing environments. Through a survey of field‐collected pea aphids, we found a negative correlation between H. defensa frequencies and average daily temperatures across North American locales, fitting expectations for reduced symbiont benefits under warm climates. Based on these findings, we propose that rising global temperatures could promote the widespread breakdown of defensive mutualisms, a prospect with implications for both human and ecosystem health.
Recent insights into cotton functional genomics: progress and future perspectives
Authors: Ashraf Javaria , Zuo Dongyun , Wang Qiaolian , Malik Waqas , Zhang Youping , Abid Muhammad Ali , Cheng Hailiang , Yang Qiuhong , Song Guoli 
Published: October 2017

SummaryFunctional genomics has transformed from futuristic concept to well‐established scientific discipline during the last decade. Cotton functional genomics promise to enhance the understanding of fundamental plant biology to systematically exploit genetic resources for the improvement of cotton fibre quality and yield, as well as utilization of genetic information for germplasm improvement. However, determining the cotton gene functions is a much more challenging task, which has not progressed at a rapid pace. This article presents a comprehensive overview of the recent tools and resources available with the major advances in cotton functional genomics to develop elite cotton genotypes. This effort ultimately helps to filter a subset of genes that can be used to assemble a final list of candidate genes that could be employed in future novel cotton breeding programme. We argue that next stage of cotton functional genomics requires the draft genomes refinement, re‐sequencing broad diversity panels with the development of high‐throughput functional genomics tools and integrating multidisciplinary approaches in upcoming cotton improvement programmes.
Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)
Authors: Angrimani DSR , Nagai KK , Rui BR , Bicudo LC , Losano JDA , Brito MM , Francischini MCP , Nichi M 
Published: October 2017

ContentsCooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.
Identification and functional characterization of diterpene synthases for triptolide biosynthesis from Tripterygium wilfordii
Authors: Su Ping , Guan Hongyu , Zhao Yujun , Tong Yuru , Xu Meimei , Zhang Yifeng , Hu Tianyuan , Yang Jian , Cheng Qiqing , Gao Linhui , Liu Yujia , Zhou Jiawei , Peters Reuben J. , Huang Luqi , Gao Wei 
Published: October 2017

SummaryTripterygium wilfordii, which has long been used as a medicinal plant, exhibits impressive and effective anti‐inflammatory, immunosuppressive and anti‐tumor activities. The main active ingredients are diterpenoids and triterpenoids, such as triptolide and celastrol, respectively. A major challenge to harnessing these natural products is that they are found in very low amounts in planta. Access has been further limited by the lack of knowledge regarding their underlying biosynthetic pathways, particularly for the abeo‐abietane tri‐epoxide lactone triptolide. Here suspension cell cultures of T. wilfordii were found to produce triptolide in an inducible fashion, with feeding studies indicating that miltiradiene is the relevant abietane olefin precursor. Subsequently, transcriptome data were used to identify eight putative (di)terpene synthases that were then characterized for their potential involvement in triptolide biosynthesis. This included not only biochemical studies which revealed the expected presence of class II diterpene cyclases that produce the intermediate copalyl diphosphate (CPP), along with the more surprising finding of an atypical class I (di)terpene synthase that acts on CPP to produce the abietane olefin miltiradiene, but also their subcellular localization and, critically, genetic analysis. In particular, RNA interference targeting either both of the CPP synthases, TwTPS7v2 and TwTPS9v2, or the subsequently acting miltiradiene synthase, TwTPS27v2, led to decreased production of triptolide. Importantly, these results then both confirm that miltiradiene is the relevant precursor and the relevance of the identified diterpene synthases, enabling future studies of the biosynthesis of this important bioactive natural product.
MOS1 functions closely with TCP transcription factors to modulate immunity and cell cycle in Arabidopsis
Authors: Zhang Ning , Wang Zhixue , Bao Zhilong , Yang Leiyun , Wu Dianxing , Shu Xiaoli , Hua Jian 
Published: October 2017

SummaryEmerging evidence indicates a close connection between cell‐cycle progression and the plant immune responses. In Arabidopsis, MODIFIER OF snc1‐1 (MOS1) modulates a number of processes including endoreduplication and plant disease resistance, but the molecular mechanism underlying this modulation was not fully understood. Here, we provide biochemical and genetic evidence that TEOSINTE BRANCHED 1, CYCLOIDEA, PCF1 (TCP) transcription factors TCP15 and its homologues are mediators of MOS1 function in the immune response and are likely to be also involved in cell‐cycle control. MOS1 and TCP proteins have a direct physical interaction. They both bind to the promoter of the immune receptor gene SUPRESSOR OF npr1‐1, CONSTITUTIVE 1 (SNC1) and modulate its expression and consequently immune responses. MOS1 and TCP15 both affect the expression of cell‐cycle genes D‐type CYCLIN 3;1 (CYCD3;1), which may mediate the MOS1 function in cell‐cycle modulation. In addition, CYCD3;1 overexpression upregulates immune responses, and SNC1 expression. This study investigated and revealed a role for MOS1 in transcriptional regulation through TCP15 and its homologues. This finding suggests the coordination of cell‐cycle progression and plant immune responses at multiple levels.