bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell Sheets of Co-cultured Endothelial Progenitor Cells and Mesenchymal Stromal Cells Promote Osseointegration in Irradiated Rat Bone
Authors: Liu Huan , Zhou Wei , Ren Nan , Feng Zhihong , Dong Yan , Bai Shizhu , Jiao Yang , Wang Zhongshan , Zhao Yimin 
Published: June 2017

AbstractIrradiated bone has a greater risk of implant failure than nonirradiated bone. The purpose of this study was to investigate the influence of cell sheets composed of co-cultured bone marrow mesenchymal stromal cells (BMSCs) and endothelial progenitor cells (EPCs) on implant osseointegration in irradiated bone. Cell sheets (EPCs, BMSCs or co-cultured EPCs and BMSCs) were wrapped around titanium implants to make cell sheet-implant complexes. The co-cultured group showed the highest osteogenic differentiation potential in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis related genes at both mRNA and protein levels. The co-cultured cells promoted ectopic bone formation as indicated by micro-computed tomography (Micro-CT) and histological analysis. In the irradiated tibias of rats, implants of the co-cultured group showed enhanced osseointegration by Micro-CT evaluation and histological observation. Co-cultured EPCs and BMSCs also up-regulated the expression of osteogenesis related genes in bone fragments in close contact with implants. In conclusion, cell sheets of co-cultured EPCs and BMSCs could promote osseous healing around implants and are potentially useful to improve osseointegration process for patients after radiotherapy.
The homology gene BtDnmt1 is Essential for Temperature Tolerance in Invasive Bemisia tabaci Mediterranean Cryptic Species
Authors: Dai Tian-Mei , Lü Zhi-Chuang , Liu Wan-Xue , Wan Fang-Hao , Hong Xiao-Yue 
Published: June 2017

AbstractThe Bemisia tabaci Mediterranean (MED) cryptic species has been rapidly invading most parts of the world owing to its strong ecological adaptability, particularly its strong resistance to temperature stress. Epigenetic mechanisms play important roles in mediating ecological plasticity. In particular, DNA methylation has been the focus of attempts to understand the mechanism of phenotypic plasticity. The relationship between temperature and DNA methylation and how it affects the adaptability of invasive insects remain unknown. To investigate the temperature resistance role of DNA methyltransferase 1 (Dnmt1) in MED, we cloned and sequenced BtDnmt1 homology and identified its functions under various temperature conditions. The full-length cDNA of MED BtDnmt1 homology was 5,958 bp and has a 4,287 bp open reading frame that encodes a 1,428-amino-acid protein. BtDnmt1 mRNA expression levels were significantly down-regulated after feeding with dsRNA. Furthermore, after feeding with dsBtDnmt1, the MED adults exhibited significantly higher mortality under temperature stress conditions than the controls, suggesting that MED BtDnmt1 homology plays an essential role in the temperature tolerance capacity of MED. Our data improve our understanding of the temperature resistance and temperature adaptability mechanisms that have allowed the successful invasion and colonization of various environments by this alien species.
Effect of climate warming on the annual terrestrial net ecosystem CO2 exchange globally in the boreal and temperate regions
Authors: Zhang Zhiyuan , Zhang Renduo , Cescatti Alessandro , Wohlfahrt Georg , Buchmann Nina , Zhu Juan , Chen Guanhong , Moyano Fernando , Pumpanen Jukka , Hirano Takashi , Takagi Kentaro , Merbold Lutz 
Published: June 2017

AbstractThe net ecosystem CO2exchange is the result of the imbalance between the assimilation process (gross primary production, GPP) and ecosystem respiration (RE). The aim of this study was to investigate temperature sensitivities of these processes and the effect of climate warming on the annual terrestrial net ecosystem CO2exchange globally in the boreal and temperate regions. A database of 403 site-years of ecosystem flux data at 101 sites in the world was collected and analyzed. Temperature sensitivities of rates of RE and GPP were quantified withQ10, defined as the increase of RE (or GPP) rates with a temperature rise of 10 °C. Results showed that on the annual time scale, the intrinsic temperature sensitivity of GPP (Q10sG) was higher than or equivalent to the intrinsic temperature sensitivity of RE (Q10sR).Q10sGwas negatively correlated to the mean annual temperature (MAT), whereasQ10sRwas independent of MAT. The analysis of the current temperature sensitivities and net ecosystem production suggested that temperature rise might enhance the CO2sink of terrestrial ecosystems both in the boreal and temperate regions. In addition, ecosystems in these regions with different plant functional types should sequester more CO2with climate warming.
The Role of TRPC6 in the Neuroprotection of Calycosin Against Cerebral Ischemic Injury
Authors: Guo Chao , Ma Yongyuan , Ma Shanbo , Mu Fei , Deng Jiao , Duan Jialin , Xiong Lize , Yin Ying , Wang Yanhua , Xi Miaomaio , Wen Aidong 
Published: June 2017

AbstractOur previous studies have provided evidences that calycosin can protect the brain from ischemia/reperfusion injury, but its mechanisms is not fully understand. Transient receptor potential canonical 6 (TRPC6) has a critical role in promoting neuronal survival against cerebral ischemic injury. The aim of the present study is to test whether calycosin protects against cerebral ischemic injury through TRPC6-CREB pathway. In vivo, rats were subjected to transient middle cerebral artery occlusion (MCAO) for 2 h and then treated with different doses of calycosin at the onset of reperfusion. In vitro, primary cultured neurons were treated by calycosin, then exposed to 2 h oxygen glucose deprivation (OGD) followed by 24 h reoxygenation. Our results showed that treatment with calycosin protected against ischemia-induced damages by increasing TRPC6 and P-CREB expression and inhibiting calpain activation. The neuroprotection effect of calycosin was diminished by inhibition or knockdown of TRPC6 and CREB. These findings indicated that the potential neuroprotection mechanism of calycosin was involved with TRPC6-CREB pathway.
Genome-wide survey of switchgrass NACs family provides new insights into motif and structure arrangements and reveals stress-related and tissue-specific NACs
Authors: Yan Haidong , Zhang Ailing , Ye Yuntian , Xu Bin , Chen Jing , He Xiaoyan , Wang Chengran , Zhou Sifan , Zhang Xinquan , Peng Yan , Ma Xiao , Yan Yanhong , Huang Linkai 
Published: June 2017

AbstractNAC proteins comprise of a plant-specific transcription factor (TF) family and play important roles in plant development and stress responses. Switchgrass (Panicum virgatum) is the prime candidate and model bioenergy grass across the world. Excavating agronomically valuable genes is important for switchgrass molecular breeding. In this study, a total of 251 switchgrass NAC (PvNACs) family genes clustered into 19 subgroups were analyzed, and those potentially involved in stress response or tissue-specific expression patterns were pinpointed. Specifically, 27 PvNACs were considered as abiotic stress-related including four membrane-associated ones. Among 40 tissue-specific PvNACs expression patterns eight factors were identified that might be relevant for lignin biosynthesis and/or secondary cell wall formation. Conserved functional domains and motifs were also identified among the PvNACs and potential association between these motifs and their predicted functions were proposed, that might encourage experimental studies to use PvNACs as possible targets to improve biomass production and abiotic stress tolerance.
An Alcaligenes strain emulates Bacillus thuringiensis producing a binary protein that kills corn rootworm through a mechanism similar to Cry34Ab1/Cry35Ab1
Authors: Yalpani Nasser , Altier Dan , Barry Jennifer , Kassa Adane , Nowatzki Timothy M. , Sethi Amit , Zhao Jian-Zhou , Diehn Scott , Crane Virginia , Sandahl Gary , Guan Rongjin , Poland Brad , Perez Ortega Claudia , Nelson Mark E. , Xie Weiping , Liu Lu , Wu Gusui 
Published: June 2017

AbstractCrops expressingBacillus thuringiensis(Bt)-derived insecticidal protein genes have been commercially available for over 15 years and are providing significant value to growers. However, there remains the need for alternative insecticidal actives due to emerging insect resistance to certainBtproteins. A screen of bacterial strains led to the discovery of a two-component insecticidal protein named AfIP-1A/1B from anAlcaligenes faecalisstrain. This protein shows selectivity against coleopteran insects including western corn rootworm (WCR). Transgenic maize plants expressingAfIP-1A/1Bdemonstrate strong protection from rootworm injury. Surprisingly, although little sequence similarity exists to known insecticidal proteins, efficacy tests using WCR populations resistant to two different Cry proteins show that AfIP-1A/1B and mCry3A differ in their mode of action while AfIP-1A/1B and the binary Cry34Ab1/Cry35Ab1 protein share a similar mode. These findings are supported by results of competitive binding assays and the similarity of the x-ray structure of AfIP-1A to Cry34Ab1. Our work indicates that insecticidal proteins obtained from a non-Btbacterial source can be useful for developing genetically modified crops and can function similarly to familiar proteins fromBt.
A Risk Model based on Ultrasound, Ultrasound Elastography, and Histologic Parameters for Predicting Axillary Lymph Node Metastasis in Breast Invasive Ductal Carcinoma
Authors: Li Xiao-Long , Xu Hui-Xiong , Li Dan-Dan , He Ya-Ping , Yue Wen-Wen , Xu Jun-Mei , Liu Bo-Ji , Sun Li-Ping , Fang Lin 
Published: June 2017

AbstractTo develop a risk model for predicting axillary lymph node metastasis (LNM) in patients with breast invasive ductal carcinoma (IDCs) using ultrasound (US), US elastography of virtual touch tissue imaging (VTI) and virtual touch tissue imaging & quantification (VTIQ), and histologic parameters. This study included 162 breast IDCs in 162 patients. Univariate and multivariate analyses were used to identify the risk factors and a risk model was created. The results found that 64 (39.5%) of 162 patients had axillary LNMs. The risk score (RS) for axillary LNM was defined as following: RS = 1.3 × (if lesion size ≥20 mm) + 2.6 × (if taller than wide shape) + 2.2 × (if VTI score ≥5) + 3.9 × (if histological grade III) + 1.9 × (if positive C-erbB-2). The rating system was divided into 6 stages (i.e. Stage I, Stage II, Stage III, Stage IV, Stage V, and Stage VI) and the associated risk rates in terms of axillary LNM were 0% (0/19), 6.1% (2/33), 7.7% (3/39), 65.5% (19/29), 92.3% (24/26), and 100% (16/16), respectively. The risk model for axillary LNM established in the study may facilitate subsequent treatment planning and management in patients with breast IDCs.
Early changes in glioblastoma metabolism measured by MR spectroscopic imaging during combination of anti-angiogenic cediranib and chemoradiation therapy are associated with survival
Authors: Andronesi Ovidiu C. , Esmaeili Morteza , Borra Ronald J. H. , Emblem Kyrre , Gerstner Elizabeth R. , Pinho Marco C. , Plotkin Scott R. , Chi Andrew S. , Eichler April F. , Dietrich Jorg , Ivy S. Percy , Wen Patrick Y. , Duda Dan G. , Jain Rakesh , Rosen Bruce R. , Sorensen Gregory A. , Batchelor Tracy T. 
Published: June 2017

AbstractPrecise assessment of treatment response in glioblastoma during combined anti-angiogenic and chemoradiation remains a challenge. In particular, early detection of treatment response by standard anatomical imaging is confounded by pseudo-response or pseudo-progression. Metabolic changes may be more specific for tumor physiology and less confounded by changes in blood–brain barrier permeability. We hypothesize that metabolic changes probed by magnetic resonance spectroscopic imaging can stratify patient response early during combination therapy. We performed a prospective longitudinal imaging study in newly diagnosed glioblastoma patients enrolled in a phase II clinical trial of the pan-vascular endothelial growth factor receptor inhibitor cediranib in combination with standard fractionated radiation and temozolomide (chemoradiation). Forty patients were imaged weekly during therapy with an imaging protocol that included magnetic resonance spectroscopic imaging, perfusion magnetic resonance imaging, and anatomical magnetic resonance imaging. Data were analyzed using receiver operator characteristics, Cox proportional hazards model, and Kaplan–Meier survival plots. We observed that the ratio of total choline to healthy creatine after 1 month of treatment was significantly associated with overall survival, and provided as single parameter: (1) the largest area under curve (0.859) in receiver operator characteristics, (2) the highest hazard ratio (HR = 85.85, P = 0.006) in Cox proportional hazards model, (3) the largest separation (P = 0.004) in Kaplan–Meier survival plots. An inverse correlation was observed between total choline/healthy creatine and cerebral blood flow, but no significant relation to tumor volumetrics was identified. Our results suggest that in vivo metabolic biomarkers obtained by magnetic resonance spectroscopic imaging may be an early indicator of response to anti-angiogenic therapy combined with standard chemoradiation in newly diagnosed glioblastoma.
Comparison of Specific Prediction Equations to Estimate Peak Oxygen Uptake in Obese Women
Authors: Racil Ghazi , Lemaire Christine , Dubart Alain-Eric , Debeaumont David , Castres Ingrid , Coquart Jérémy B. 
Published: June 2017

AbstractThe aim of the current study was to compare 2 equations to predict peak oxygen uptake (V̇O2peak) in obese women, according to their obesity class. 92 maximal cardiopulmonary exercise testing sets (CPET with initial and subsequent increments set to achieve an exercise duration between 8–12 min) were retrospectively analysed. These CPET were divided into 3 groups according to the women body mass indexes (BMI): class 1 (30 kg.m−2≤BMI<35 kg.m−2, n=22), class 2 (35 kg.m−2≤BMI<40 kg.m−2, n=36) or class 3 (BMI≥40 kg.m−2, n=34). Each participant’s V̇O2peak was predicted from 2 prediction equations (from Wasserman et al.'s and Debeaumont et al.'s equations) and compared with the actual V̇O2peak. Moreover, the correlations between these values were studied, and the accuracy of the predictions was analysed. Only predicted V̇O2peak from the Debeaumont et al.'s equation was not significantly different from the actual V̇O2peak in the women in obesity class 3 (p=0.89). Moreover, significant correlation was found between these values (p<0.001, r=0.68). The bias and the 95% limits of agreement represented -3.2±34.0%. In women in obesity class 3, Debeaumont et al.'s equation may be the accurate one to predict V̇O2peak. However, the accuracy of predictions is low. Consequently, to improve this accuracy, new prediction equations for obese women are required according to the obesity class.
Chirurgische Möglichkeiten der Glaukomtherapie
Authors: Dietlein Thomas , Rosentreter André 
Published: June 2017

ZusammenfassungDie Glaukomchirurgie erfährt in den letzten Jahren einen Innovationsschub insbesondere bedingt durch neue Mikroimplantate, die den Kammerwasserabfluss über transsklerale Wege, über den Schlemm-Kanal oder den Suprachoroidalraum verbessern sollen und eine sehr gute Kombinierbarkeit mit der modernen Kleinschnittkataraktchirurgie aufweisen. Auch lange bestehende glaukomchirurgische Verfahren, wie die Trabekulektomie oder die Drainageimplantatchirurgie erleben eine Aufwertung durch eine Verbesserung ihres Risiko-Nutzen-Profils, die in vermehrter chirurgischer Erfahrung und detaillierten chirurgischen Modifikationen begründet ist. Bei aller Euphorie über die chirurgischen Innovationen muss mit kritischem Abstand abgewartet werden, welches der neueren chirurgischen Verfahren langfristig dem klinischen Vergleich mit den etablierten Verfahren standhält.
One-bit compressive sensing of dictionary-sparse signals
Authors: Baraniuk R , Foucart S , Needell D , Plan Y , Wootters M 
Published: June 2017

Abstract
One-bit compressive sensing has extended the scope of sparse recovery by showing that sparse signals can be accurately reconstructed even when their linear measurements are subject to the extreme quantization scenario of binary samples—only the sign of each linear measurement is maintained. Existing results in one-bit compressive sensing rely on the assumption that the signals of interest are sparse in some fixed orthonormal basis. However, in most practical applications, signals are sparse with respect to an overcomplete dictionary, rather than a basis. There has already been a surge of activity to obtain recovery guarantees under such a generalized sparsity model in the classical compressive sensing setting. Here, we extend the one-bit framework to this important model, providing a unified theory of one-bit compressive sensing under dictionary sparsity. Specifically, we analyze several different algorithms—based on convex programming and on hard thresholding—and show that, under natural assumptions on the sensing matrix (satisfied by Gaussian matrices), these algorithms can efficiently recover analysis–dictionary-sparse signals in the one-bit model.
Observational constraints to boxy/peanut bulge formation time
Authors: Pérez I. , Martínez-Valpuesta I. , Ruiz-Lara T. , de Lorenzo-Caceres A. , Falcón-Barroso J. , Florido E. , González Delgado R. M. , Lyubenova M. , Marino R. A. , Sánchez S. F. , Sánchez-Blázquez P. , van de Ven G. , Zurita A. 
Published: June 2017

Abstract
Boxy/peanut bulges are considered to be part of the same stellar structure as bars and both could be linked through the buckling instability. The Milky Way is our closest example. The goal of this Letter is to determine if the mass assembly of the different components leaves an imprint in their stellar populations allowing the estimation the time of bar formation and its evolution. To this aim, we use integral field spectroscopy to derive the stellar age distributions, SADs, along the bar and disc of NGC 6032. The analysis clearly shows different SADs for the different bar areas. There is an underlying old (≥12 Gyr) stellar population for the whole galaxy. The bulge shows star formation happening at all times. The inner bar structure shows stars of ages older than 6 Gyr with a deficit of younger populations. The outer bar region presents an SAD similar to that of the disc. To interpret our results, we use a generic numerical simulation of a barred galaxy. Thus, we constrain, for the first time, the epoch of bar formation, the buckling instability period and the posterior growth from disc material. We establish that the bar of NGC 6032 is old, formed around 10 Gyr ago while the buckling phase possibly happened around 8 Gyr ago. All these results point towards bars being long-lasting even in the presence of gas.
No Ly α emitters detected around a QSO at z = 6.4: Suppressed by the QSO?
Authors: Goto Tomotsugu , Utsumi Yousuke , Kikuta Satoshi , Miyazaki Satoshi , Shiki Kensei , Hashimoto Tetsuya 
Published: June 2017

Abstract
Understanding how quasars’ (QSOs’) ultraviolet (UV) radiation affects galaxy formation is vital to our understanding of the reionization era. Using a custom-made narrow-band filter, NB906, on Subaru/Suprime-Cam, we investigated the number density of Ly α emitters (LAEs) around a QSO at z = 6.4. To date, this is the highest redshift narrow-band observation, where LAEs around a luminous QSO are investigated. Due to the large field of view of Suprime-Cam, our survey area is ∼5400 cMpc2, much larger than previous studies at z = 5.7 (∼200 cMpc2). In this field, we previously found a factor of 7 overdensity of Lyman break galaxies. Based on this, we expected to detect ∼100 LAEs down to NB906 = 25 ABmag. However, our 6.4 h exposure found none. The obtained upper limit on the number density of LAEs is more than an order lower than the blank fields. Furthermore, this lower density of LAEs spans a large scale of 10 pMpc across. A simple argument suggests that a strong UV radiation from the QSO can suppress star formation in haloes with Mvir &lt; 1010 M⊙ within a pMpc from the QSO, but the deficit at the edge of the field (5 pMpc) remains to be explained.
The origin, diversification and adaptation of a major mangrove clade (Rhizophoreae) revealed by whole-genome sequencing
Authors: Xu Shaohua , He Ziwen , Zhang Zhang , Guo Zixiao , Guo Wuxia , Lyu Haomin , Li Jianfang , Yang Ming , Du Zhenglin , Huang Yelin , Zhou Renchao , Zhong Cairong , Boufford David E , Lerdau Manuel , Wu Chung-I , Duke Norman C. ,   The International Mangrove Consortium, Shi Suhua 
Published: June 2017

Abstract
Mangroves invade some very marginal habitats for woody plants—at the interface between land and sea. Since mangroves anchor tropical coastal communities globally, their origin, diversification and adaptation are of scientific significance, particularly at a time of global climate change. In this study, a combination of single-molecule long reads and the more conventional short reads are generated from Rhizophora apiculata for the de novo assembly of its genome to a near chromosome level. The longest scaffold, N50 and N90 for the R. apiculata genome, are 13.3 Mb, 5.4 Mb and 1.0 Mb, respectively. Short reads for the genomes and transcriptomes of eight related species are also generated. We find that the ancestor of Rhizophoreae experienced a whole-genome duplication ∼70 Myrs ago, which is followed rather quickly by colonization and species diversification. Mangroves exhibit pan-exome modifications of amino acid (AA) usage as well as unusual AA substitutions among closely related species. The usage and substitution of AAs, unique among plants surveyed, is correlated with the rapid evolution of proteins in mangroves. A small subset of these substitutions is associated with mangroves’ highly specialized traits (vivipary and red bark) thought to be adaptive in the intertidal habitats. Despite the many adaptive features, mangroves are among the least genetically diverse plants, likely the result of continual habitat turnovers caused by repeated rises and falls of sea level in the geologically recent past. Mangrove genomes thus inform about their past evolutionary success as well as portend a possibly difficult future.
Distribution-Sensitive Multidimensional Poverty Measures
Authors: Datt Gaurav 
Published: June 2017

AbstractThis paper presents axiomatic arguments to make the case for distribution-sensitive multidimensional poverty measures. The commonly used counting measures violate the strong transfer axiom, which requires regressive transfers to be unambiguously poverty increasing, and they are also invariant to changes in the distribution of a given set of deprivations among the poor. The paper appeals to strong transfer as well as an additional cross-dimensional convexity property to offer axiomatic justification for distribution-sensitive multidimensional poverty measures. Given the nonlinear structure of these measures, it is also shown how the problem of an exact dimensional decomposition can be solved using Shapley decomposition methods to assess dimensional contributions to poverty. An empirical illustration for India highlights distinctive features of the distribution-sensitive measures.