bluuebunny/crossref_metadata_2025_split huggingface dataset:
The peace project: creating elementary school peace spaces and places
Authors: McIntyre Miller Whitney , Abdou Annmary S. , Clancy Cristie Suzukawa , Stephens Kelly 
Published: October 2024

IntroductionThis pilot case study paper demonstrates how school programming can be aligned and enhanced to better create a climate of peace on an elementary school campus by utilizing an integral peace leadership lens. Working collectively as a Peace Leadership Advisory Group, the elementary school leadership team, and the university research team helped to align existing programming and explore and implement new programming to create a comprehensive plan for bringing peace ideas together at the elementary school level.MethodsThis pilot was a single case study that utilized Participatory Action Research. Data were collected through observation, survey, and interviews with school leadership and analyzed using thematic analysis, descriptive statistics, and grounded theory methods, respectively.ResultsThe pilot study revealed that the efforts to build peace on campus were successful overall, with students and staff having a positive experience with peace programming throughout the academic year.DiscussionThe findings indicate that aligning existing programming as a way to frame a culture of peace and then supplementing that programming with additional activities serves as a way to unite a campus around the idea of peace.
The influence of community social capital on pre-service teachers’ career calling: the mediating role of career exploration and the moderating effect of agreeableness
Authors: Su Xiaomin , Ahmad Anuar , Alias Jamsari 
Published: October 2024

IntroductionThis study aims to explore the mechanism by which community social capital influences pre-service teachers’ career calling, while also examining the mediating role of career exploration and the moderating effect of agreeableness.MethodsData were collected from 455 pre-service teachers at a local undergraduate normal college in Guangdong Province, and a structural equation model was employed for analysis.ResultsThe findings revealed a significant positive relationship between community social capital and career calling, with career exploration serving as a mediating variable between them. Additionally, agreeableness moderated the relationship between community social capital and career exploration.DiscussionThese results provide valuable theoretical and practical support for colleges and universities in developing community service-learning education programs that enhance pre-service teachers’ career exploration, address their individualized requirements, and cultivate their career calling.
Knowledge and awareness toward human papillomavirus vaccination among Saudi female nursing students
Authors: Alanazi Amal Khulaif , Kayal Eithar , Alanzi Shahad , Al Hodian Hanadi , Bin Rusayes Alhanouf 
Published: October 2024

IntroductionHuman Papillomavirus is a sexually transmitted agent, causing cervical cancer. In Saudi Arabia, cervical cancer is ranked as the ninth most common carcinoma in women. HPV vaccine is an effective prevention method against HPV high-risk types such as HPV 18 and 16. Research on HPV vaccine knowledge and awareness is limited due to the lack of extensive data reportage on HPV and cervical cancer cases among Saudi women.AimThis study was aimed to determine the knowledge and awareness of human papillomavirus vaccination among Saudi nursing female students.MethodsThis study was cross-sectional and included (n = 114) participants. The study used an online survey, which included demographical variables and the HPV knowledge scale. The data were collected from October 10, 2023, until January 3, 2024. Descriptive data, Mann-Whitney Z-tests and nonparametric tests were used to analyze the study's findings.ResultsThe study participants’ mean age was 20.8 years (SD 1.6). Most students (72%) did not receive the HPV vaccine. The overall mean HPV knowledge was 10.0 (SD 7.08). The HPV knowledge subscales showed poor levels of knowledge of HPV infection, screening, and vaccines: 5.15 (SD 3.87), 1.39 (SD 1.34), and 2.06 (SD 1.87), respectively.DiscussionIn conclusion, Saudi Arabia having a predominantly youthful population, it is crucial to implement educational programs that improve the understanding and awareness of HPV infection among nursing students and other health professionals. There is a necessity to establish impactful awareness campaigns and integrate interventional research to inform health professionals and the public about the disease and dispel misunderstandings and cultural beliefs about HPV and HPV vaccines to prevent cervical cancers among young females.
Psychological impact of autologous hematopoietic stem cell transplantation in systemic sclerosis patients and influence of resilience
Authors: Schmalzing Marc , Gernert Michael , Fröhlich Matthias , Henes Jörg , Schwindl Nathalie , Zerhusen Leona , Berthold Lukas , Hewig Johannes , Kübler Andrea , Pecher Ann-Christin , Kleih-Dahms Sonja , Strunz Patrick-Pascal , Ziebell Philipp 
Published: October 2024

ObjectiveIn severe cases of systemic sclerosis (SSc), autologous hematopoietic stem cell transplantation (aHSCT) is superior compared to cyclophosphamide. But treatment related morbidity and mortality have to be considered. To date, data on major physical and psychological impacts of aHSCT are scarce. Therefore, subjectively experienced physical and psychological impact of aHSCT and exploration of internal and external factors helping to cope with aHSCT was assessed.MethodsRetrospective assessment of physical and psychological variables in an SSc cohort after aHSCT to describe: Health-related quality of life (HRQL), SSc-associated impairment, coping strategies, body image, and resilience. Additionally, semi-structured interviews were conducted and analyzed via mixed methods qualitative content analysis.ResultsThirty-two patients were included. HRQL correlated with impairment due to SSc and with depressive coping. An unfavorable body image correlated with reduced HRQL and increased impairment but improves after aHSCT. Patients with good resilience had a better HRQL, less depressive coping, and less SSc-associated impairment. The semi-structured interviews revealed that resilience is important for a successful disease management as patients with higher resilience were more satisfied with aHSCT, patients with lower resilience would have wished for more psychological support. Thirty-one patients would recommend aHSCT to other patients.ConclusionA transient negative impact of aHSCT on mental well-being is present but can be relieved by a team specialized to aHSCT. Psychological support seems to be an unmet need, particularly in patients with low resilience. Patients with higher resilience described a lower negative impact caused by aHSCT and higher satisfaction after therapy.
Effect of peripheral blood lymphocyte count on the efficacy of immunotherapy combined with TKI in the treatment of advanced liver cancer
Authors: Zhao Qian , Wang Lei , Fu Huilan , Zhang Yuqin , Xie Qiankun 
Published: October 2024

Background and aimsCompared with tyrosine kinase inhibitor (TKI) monotherapy, TKI combined with PD1 can improve the therapeutic effect of liver cancer and has been widely used in clinical practice. However, there is a lack of effective biomarkers to identify patients who would benefit more from this combination therapy. Therefore, this study aimed to evaluate whether baseline lymphocyte counts can identify patients with liver cancer who would benefit from targeted immune combination therapy.MethodsData from patients with hepatocellular carcinoma (HCC) who received TKIs or TKIs in combination with PD1 between June 2018 and June 2020 were retrospectively collected. The patients were divided into high and low groups based on the median absolute count of peripheral lymphocytes before systemic therapy and differences in overall survival (OS) and progression-free survival (PFS) between TKI and TKI+PD1 were compared between the two groups.ResultsIn total, 72 patients were included in this study, with a median follow-up of 1.5 years. Both PFS and OS in the TKI+PD1 group showed a good prognostic trend (p = 0.058 and p = 0.077, respectively). Subgroup analyses based on peripheral blood lymphocyte counts showed that the combination regimen had a significant PFS and OS advantage only in patients with high peripheral blood lymphocyte counts (p = 0.036 and p = 0.031, respectively), but not in patients with low absolute peripheral blood lymphocyte counts (p = 0.819 and p = 0.913, respectively).ConclusionsPeripheral blood lymphocyte count is a simple and effective biomarker that can be used to identify patients with liver cancer who will benefit more from TKI+PD-1 combination therapy.
Immunomodulatory properties of extracellular vesicles isolated from bone marrow of patients with neuroblastoma: role of PD-L1 and HLA-G
Authors: Marimpietri Danilo , Corrias Maria Valeria , Tripodi Gino , Gramignoli Roberto , Airoldi Irma , Morandi Fabio 
Published: October 2024

IntroductionExtracellular vesicles (EVs) can be released by any cell and are crucial for cell-to-cell communications. EVs have been characterized in patients with solid and hematological tumors, where they play an important role in tumor progression and metastasis. EVs may express different surface proteins derived from the parental cells, including immunomodulatory molecules, such as HLA-G and PDL1.MethodsWe isolated EV from bone marrow (BM) samples of patients with Neuroblastoma (NB) and healthy controls and we analyzed the expression of CD56, GD2 and immune checkpoints on EV by flow cytometry. Next, we analyzed the function of T cells in vitro in the presence or absence of NB patients' BM-derived EV, in terms of proliferation and cytokine production. Finally, we analyzed the correlation between the expression of immune checkpoints on EV and the clinical outcome of patients.ResultsWe found a higher expression of CD56 on EVs derived from BM of patients with NB than in those from healthy donors (HD). However, CD56 expression was not dependent on BM infiltration of NB cells. Moreover, the analysis of GD2 expression revealed that only a small fraction of EVs was released by infiltrating NB cells, whereas the majority may derive from BM-resident cells. BM-derived EVs from NB patients display a higher expression of HLA-G and PD-L1 than those derived from HD. Nonetheless, such EVs are able to modulate T cell immune responses. We measured a robust response, in vitro, towards a common bacterial antigen, including the release of GM-CSF and proinflammatory cytokines, like IFN-a and IL-6, from mononuclear cells. Some of these immunomodulatory features are dependent on the expression of HLA-G and PD-L1, whereas others may rely on other mechanism(s). Finally, a high expression of CD56, HLA-G and PD-L1 on BM-derived EVs may represent a good prognostic factor.ConclusionsWe described the presence of HLA-G and PDL1-bearing EVs in the BM of NB patients, which may represent a mechanism performed by resident BM cells to counteract the inflammation occurring in the BM microenvironment of NB patients.
Prognostic value and immunomodulatory role of DNM1L in gastric adenocarcinoma
Authors: Zhao Zhuo , Li Lingxia , Liu Yan , Shi Lei , Yuan Meijie , Shi Hongshuo , Ji Qing , Liu Guobin , Sun Jian 
Published: October 2024

BackgroundMitochondrial fusion and fission are critical for the morphology and function of cells. DNM1L encodes dynamin-related protein 1 (DRP1), a key protein mediating mitochondrial fission, which is upregulated in a variety of cancers and is strongly associated with tumorigenesis. We aim to investigate the relationship between DNM1L and the prognosis of gastric cancer, as well as to explore the function and mechanism of DNM1L in gastric cancer (GC).Materials and methodsIn this study, we analyzed the expression differences of DNM1L in gastric cancer tissues and paracancerous tissues using The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database. This was followed by validation through tissue microarrays. We then utilized the cohort information from these microarrays to explore the relationship between DNM1L expression and gastric cancer prognosis. Furthermore, we conducted enrichment analysis to investigate the function and mechanisms of DNM1L in gastric cancer, and lastly, we performed immune cell infiltration analysis using the CIBERSORT algorithm.ResultsWe discovered that the expression of DNM1L is elevated in GC tissues. TCGA data showed that the overexpression of DNM1L was positively correlated with the T-stage of GC but not with lymph node metastasis, which was also corroborated by our immunohistochemistry experiments. Based on the Kaplan–Meier curves, the high DNM1L expression was remarkably correlated with poor overall survival in patients with GC. In addition, results of COX regression analysis indicated that high DNM1L expression was an independent prognostic factor in patients with GC. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene set enrichment analysis (GSEA) showed that DNM1L was closely associated with multiple signaling pathways and immune responses. CIBERSORT analysis indicated that increased DNM1L expression may affect the infiltration of immune cells in the tumor microenvironment.ConclusionThe results of this study indicate that DNM1L is upregulated in gastric cancer (GC) and positively correlates with the T-stage and poor prognosis of GC patients, and it plays an important role in tumor immune infiltration.
Causal relationships among perception of errors, challenges, and deliberate practice in athletes with disabilities
Authors: Sim Young Kyun , Shin Joon Ha , Kim Song Eun , Seo Eun Chul , Ha Min-Seong 
Published: October 2024

IntroductionThere is limited evidence that the psychological characteristics of athletes with disabilities are identical to those of non-disabled athletes, owing to differences in ecological traits, and there is insufficient information on how athletes with disabilities perceive disabled athletes’ perception of errors, challenges, and deliberate practice. Therefore, it is necessary to examine whether the causal model of the perception of errors, challenge, and deliberate practice will be reproduced in the same way as in non-disabled athletes. Therefore, this study aimed to verify a causal model of the perception of errors, challenges, and deliberate practice by athletes with disabilities.MethodsThe participants were 189 athletes with physical and hearing impairments (131 men and 58 women) registered with the 2023 Korea Paralympic Committee. Data were collected through a survey and the participants responded using a self-report method. The collected data were analyzed using descriptive statistics to verify normality, correlation analysis to examine relationships between variables, and structural equation modeling (SEM) to test the hypotheses.ResultsBased on SEM analysis, the results of this study showed that the causal relationships between the perception of errors, challenges, and deliberate practice were partially significant. Specifically, perception of errors and reflection positively predicted challenges, whereas burden of mistakes negatively predicted challenges. Additionally, challenges were found to have a positive effect on deliberate practice.DiscussionBy comprehensively examining the above, it can be interpreted as a major factor that can promote and reduce challenges depending on how athletes with disabilities perceive their mistakes.
A survey of the experiences of delivering physiotherapy services through telerehabilitation during the COVID-19 pandemic
Authors: Peng Tzu-Hsuan , Eng Janice J. , Harris Anne , Le Cornu Levett Catherine , Yao Jennifer , Schneeberg Amy , Pollock Courtney L. 
Published: October 2024

IntroductionPhysiotherapy services have been typically provided in-person since the profession usually involves a therapist providing hands-on assessment and treatments. The COVID-19 pandemic provided an opportunity to study physiotherapists’ adaptation to telerehabilitation (phone or videoconference).ObjectiveThis study aimed: (1) to explore how physiotherapists adapted to the transition to delivering telerehabilitation, (2) to assess physiotherapists’ perceptions of implementing telerehabilitation, and (3) to identify the challenges and facilitators of delivering telerehabilitation.MethodsThis study used an online survey distributed to physiotherapists within a large Canadian health authority. Closed-ended questions were analyzed with descriptive statistics.ResultsSeventy-five physiotherapists responded and data were collected. Compared prior to the pandemic to time during the pandemic, the use of a phone for delivering physiotherapy increased from 24.0% to 73.3% of physiotherapists while videoconference increased from 5.3% to 77.3%. Overall, the physiotherapists found videoconference to be a more effective delivery method than phone. Less than half felt that they could use videoconference to effectively treat pain (49.3%), upper extremity function (40.0%) or strength/range of motion (48.0%). Only 29.3% felt that they could effectively treat walking balance or mobility by videoconference. Technical barriers were identified with client comfort with the equipment reported by 90.7% of physiotherapists and positioning of the webcam by 76.0% of physiotherapists. A large proportion of physiotherapists agreed that they would continue the practice of telerehabilitation via phone (54.7%) and videoconference (68.0%).ConclusionThe pandemic resulted in a dramatic shift to telerehabilitation for a profession that typically provides hands-on assessments and treatments. While there was increased uptake of telerehabilitation, many physiotherapists questioned their effectiveness using telerehabilitation to undertake activities that traditionally involve manual treatments or hands-on guidance/supervision. However, physiotherapists were committed to continuing telerehabilitation to meet patients’ needs after the pandemic.
Changes in female football players’ in-season training load, intensity and physical performance: training progression matters more than accumulated load
Authors: Savolainen Eero H. J. , Ihalainen Johanna K. , Vänttinen Tomi , Walker Simon 
Published: October 2024

IntroductionThis observational study investigated: (1) potential changes in female football players’ in-season training load, intensity and physical performance, and (2) if in-season accumulated training load, intensity, or their progression are associated to changes in physical performance.MethodsThirty-five national level female players (∼21 years, n = 35) from three top-teams of the Finnish national league participated. Players performed tests at the beginning and at the end of the 27-week in-season. Tests were: 30-m sprint, countermovement jump (CMJ) and 1,200-m shuttle run, used to calculate maximal aerobic speed (MAS). Players’ external and internal training load and intensity were monitored in all on-field training sessions and official matches (3,941 data samples) using Polar Team Pro system.ResultsTraining load decreased towards the end of the in-season (p &lt; 0.05), but intensity remained stable. No changes in physical performance test results occurred from before to after in-season tests at a group level. Change of CMJ correlated negatively with accumulated training load, intensity and progression of total distance (TD) and low-intensity running distance (LIRD) (r = −0.398 to −0.599, p &lt; 0.05). Instead, development of MAS correlated positively with progression of TD and LIRD intensities (r = 0.594 and 0.503, p &lt; 0.05). Development of both CMJ and MAS correlated positively with intensity progression of very-high-intensity running distance (VHIRD) and number of accelerations and decelerations (r = 0.454–0.588, p &lt; 0.05).DiscussionReduced training load over the in-season is not detrimental for players’ physical performance when training intensity progressively increases. Intensity progression of VHIRD, moderate- and high-intensity accelerations and decelerations are indicators of both MAS and CMJ development, respectively.
Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis
Authors: Xu Zhuoran , Liu Hongwei , Zheng Xin , Cheng Xiaoxia , Wang Shao , You Guangju , Zhu Xiaoli , Zheng Min , Dong Hui , Xiao Shifeng , Zeng Li , Zeng Xiancheng , Chen Shaoying , Chen Shilong 
Published: October 2024

IntroductionClassical Muscovy duck reovirus (C-MDRV) and goose-origin Muscovy duck reovirus (Go-MDRV) infections cause “Liver white-spots disease” in Muscovy duckling and gosling. It is difficult to differentiate the infections caused by C-MDRV and Go-MDRV using conventional serological methods.MethodsSpecific primers were designed and synthesized according to σNS and λA nucleotide sequences of C-MDRV and Go-MDRV, respectively. The PCR amplified products were cloned into the pMD-18-T vector. The recombinant plasmid DNA was used to establish an SYBR Green І based duplex real-time PCR assay for the simultaneous detection and differentiation of C-MDRV and Go-MDRV using high-resolution melting (HRM) analysis. The specificity, sensitivity, and repeatability of the methodology were examined based on the optimization of the reaction system and amplification conditions.ResultsC-MDRV and Go-MDRV were identified by their distinctive melting temperatures with 84.50 ± 0.25°C for C-MDRV and 87.50 ± 0.20°C for Go-MDRV, respectively. The amplifications were specific, and other non-targeted waterfowl viruses employed in this study did not show normalized melting peaks. The intra- and inter-assay coefficients of variations were between 0.05 and 1.83%, demonstrating good repeatability. The detection limits of this assay were 51.4 copies·μl−1 for C-MDRV and 61.8 copies·μl−1 for Go-MDRV, respectively. A total of 45 clinical samples were tested by RT-qPCR, with positive rates of 15.56% for C-MDRV and 22.22% for Go-MDRV, without co-infections.DiscussionThese results suggest that this duplex RT-qPCR method is highly sensitive, specific, and reproducible. The HRM assay established in this study provides a powerful tool for the differential detection and epidemiological investigation of C-MDRV and Go-MDRV.