bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revealing the pH-dependent conformational changes in sol g 2.1 protein and potential ligands binding
Authors: Nonkhwao Siriporn , Leaokittikul Doungkamol , Patramanon Rina , Jangpromma Nisachon , Daduang Jureerut , Daduang Sakda 
Published: September 2024

AbstractSol g 2, a major protein found in the venom of the tropical fire ant (Solenopsis geminata), is well-known for its ability to bind various hydrophobic molecules. In this study, we investigate the binding activity of recombinant Sol g 2.1 protein (rSol g 2.1) with potential molecules, including (E)-β-Farnesene, α-Caryophyllene, and 1-Octen-3-ol at different pH levels (pH 7.4 and 5.5) using fluorescence competitive binding assays (FCBA). Our results revealed that Sol g 2.1 protein has higher affinity binding with these ligands at neutral pH. Relevance to molecular docking and molecular dynamics simulations were utilized to provide insights into the stability and conformational dynamics of Sol g 2.1 and its ligand complexes. After simulation, we found that Sol g 2.1 protein has higher affinity binding with these ligands as well as high structural stability at pH 7.4 than at an acidic pH level, indicating by RMSD, RMSF, Rg, SASA, and principal component analysis (PCA). Additionally, the Sol g 2.1 protein complexes at pH 7.4 showed significantly lower binding free energy (∆Gbind) and higher total residue contributions, particularly from key non-polar amino acids such as Trp36, Met40, Cys62, and Ile104, compared to the lower pH environment. These explain why they exhibited higher binding affinity than the lower pH. Therefore, we suggested that Sol g 2.1 protein is a pH-responsive carrier protein. These findings also expand our understanding of protein–ligand interactions and offer potential avenues for the development of innovative drug delivery strategies targeting Sol g 2.1 protein.
Exogenous selenium mitigates cadmium uptake and accumulation in two rice (Oryza sativa L.) varieties in cadmium-contaminated soil
Authors: Wu Wenjiang , Qi Deqiang , Chen Yalong , Wang Jiaqi , Zhang Ganggang , Wang Qinghua , Niu Hongbin , Zhao Quanzhi , Peng Ting 
Published: September 2024

AbstractRice grown in cadmium (Cd)-contaminated soil, is a potential threat to human health, but exogenous selenium (Se) application on rice can mitigate Cd toxicity. However, the mechanisms underlying Se mitigation of Cd stress in ratoon rice (RR) are still poorly understood. We conducted a pot experiment with moderate Cd-contaminated yellow–brown paddy soil on two rice varieties ‘Taoyouxiangzhan’ (TX) and ‘Liangyou 6326’(LY). For all treatments, 1.0 mg kg−1 sodium selenite solution was added to soil. Treatment T1 was sodium selenite only, and in the other treatments 100 mg L−1 Se solution was sprayed on the leaves at seedling stage (T2), at tillering stage (T3), and in early anthesis stage (T4). Se treatments decreased Cd accumulation in rice grains and herbage. Under foliar spraying 100 mg L−1 Se at the seedling + 1.0 mg kg−1 Se in soil (T2), leaf Cd content decreased 16.95% in the current season and grains content decreased 46.67% in the subsequent season. Furthermore, grain Se content increased 0.94 mg kg−1 for the TX variety combined with the analysis of Cd bio-accumulation factor in grains, and Se treatments effectively decreased Cd grain concentrations due to reduced Cd translocation from roots to grains. TX variety rice showed a more pronounced response to Se treatments than LY.
Astodrimer sodium nasal spray forms a barrier to SARS-CoV-2 in vitro and preserves normal mucociliary function in human nasal epithelium
Authors: Paull Jeremy R. A. , Luscombe Carolyn A. , Seta Aynaz , Heery Graham P. , Bobardt Michael D. , Gallay Philippe A. , Constant Samuel , Castellarnau Alex 
Published: September 2024

AbstractCOVID-19 remains a severe condition for many including immunocompromised individuals. There remains a need for effective measures against this and other respiratory infections, which transmit via virus-laden droplets that reach the nasal or oral mucosae. Nasal sprays offer potential protection against viruses. Such formulations should preserve normal nasal mucociliary function. The antiviral barrier efficacy and effects on mucociliary function of astodrimer sodium nasal spray (AS-NS) were evaluated and compared with other available nasal sprays—low pH hydroxypropyl methylcellulose (HPMC-NS), iota-carrageenan (Carr-NS), nitric oxide (NO-NS), and povidone iodine (PI-NS). Assays simulated clinical conditions. Antiviral barrier function and cell viability were assessed in airway cell monolayers, while a model of fully differentiated human nasal epithelium (MucilAir™) was utilized to evaluate tissue integrity, cytotoxicity, cilia beating frequency, and mucociliary clearance. AS-NS reduced infectious virus in cell monolayers and demonstrated a benign cytotoxicity profile. In human nasal epithelium ex vivo, AS-NS had no impact on mucociliary function (cilia beating nor mucociliary clearance). Carr-NS, HPMC-NS, NO-NS and PI-NS demonstrated limited antiviral effects, while HPMC-NS caused inhibition of mucociliary function. Astodrimer sodium nasal spray demonstrates an acceptable nonclinical efficacy and safety profile as a barrier nasal spray against respiratory viral infection in the nasal cavity.
Effect of lignin contents in raw lignocellulosic materials on the efficiency of modification process and the removal of Fe3+, Ca2+, and Mg2+ in aqueous solution
Authors: Nguyen Dang T. 
Published: September 2024

Abstract
The different chemical agents such as ethanol, NaOH/water, and NaOH/ethanol-water were investigated to separate the lignin component in the three kinds of raw lignocellulosic materials (cotton fiber, acacia auriculiformis sawdust, coconut shell powder) in the Souther Vietnam. The lignin contents were evaluated based on the Kappa number. The obtained results revealed that the 0.2 mol/L (M) solution of NaOH in the ethanol:water v/v ratio of 7:3 was the good delignified agent and the lignin contents reduced in the sequence as follow: coconut shell powder > acacia auriculiformis sawdust > cotton fiber. The lignin contents in raw materials have significant influence on the efficiency of modification process by using citric acid (CA) in the sequence as follow: modified cotton fiber > modified acacia auriculiformis sawdust > modified coconut shell powder. When using a fixed-bed column, the obtained results showed that modified materials could remove Fe3+, Ca2+, and Mg2+ with the good efficiency in some specific conditions.
Introduction and Context
Authors: Hayward Benjamin 
Published: September 2024

Abstract
This introductory chapter first sets out this book’s focus, namely the conflict of laws in international commercial arbitration. Its argument is twofold: first, that the discretion reposed in arbitrators resolving conflict of laws questions concerning the substantive law governing the merits in international commercial arbitration is too wide; and, second, that a bright-line test based on the concept of the closest connection is a preferable mechanism by which these questions can be resolved. In essence, this book critiques discretion in arbitral procedure in the specific arbitral conflicts context. The remainder of the chapter establishes the background to this book’s analysis. It takes as its starting point the preferred status of international commercial arbitration, and from this point introduces arbitral conflicts of laws—this book’s key subject-matter.
Identified Issues in the Current Regulatory Regime
Authors: Hayward Benjamin 
Published: September 2024

Abstract
This chapter identifies problematic features of the current regulatory regime for conflicts of laws in international commercial arbitration. The current regulatory regime evidences broad arbitral discretion in the ability of arbitrators applying pure voie indirecte provisions to adopt any conflicts rule considered ‘appropriate’, ‘applicable’, or ‘authoritative’; through the pure voie directe’s wide freedom to directly choose the ‘appropriate’, ‘most appropriate’, or ‘applicable’ law; through the facilitative role that conflict of laws rules may play within the pure and guided voie directe; and through arbitrators’ use of implicit conflicts analyses under pure voie directe provisions. The chapter then analyzes the justifications for arbitral discretion in resolving conflicts of laws, following with theoretical and practical critiques of the current regulatory regime.
A Proposed Solution—A Bright-Line Test
Authors: Hayward Benjamin 
Published: September 2024

Abstract
This chapter demonstrates that reform of the current regulatory regime for arbitral conflicts of laws should be via a bright-line test. These are accurate: their correct application generates a particular result, so they give ‘clearer notice’ to arbitrating and commercial parties regarding the applicable law. Adopting a test for arbitral conflicts would not be over-regulating. The wholesale adoption of detailed procedural rules—the kind of State court procedure arbitration seeks to avoid—is different from appropriately regulating a particular procedural question for which broad discretions do not quite fit. What is advocated here falls far short of an unachievable universal arbitration law, the New York Convention showing that harmonization attempts are feasible over a modest and appropriate range of procedural issues.
A Proposed Closest Connection Test—The (Modified) Art 4 Rome Convention Rule
Authors: Hayward Benjamin 
Published: September 2024

Abstract
Chapter 5 recommended the closest connection test as the basis for reforming conflicts of laws in international commercial arbitration. This chapter analyzes six distinct variations of the closest connection test. It then proposes a (modified) Art 4 Rome Convention rule as the specific basis for reform. The three modifications recommended are: addition of the word ‘clearly’ or ‘manifestly’ to the original text of Art 4(5) Rome Convention, to confirm the strength of the Art 4(2) Rome Convention characteristic performance presumption; substitution of a power to apply rules of law, for the power to apply only law, to facilitate the &lt;i&gt;lex mercatoria&lt;/i&gt;’s application in cases involving evenly distributed connecting factors; and substitution of the case criterion for the original contract criterion, as the matter to which the applicable (rules of) law must be connected.
Further Psychometric Testing of the Chest Pain Conception Questionnaire in a Racially and Ethnically Diverse Sample
Authors: Blakeman John R. , Kim MyoungJin , Eckhardt Ann L. 
Published: September 2024

Background
The Chest Pain Conception Questionnaire was developed to measure the lay public's conceptions of chest pain related to acute coronary syndrome.

Objective
The purpose of this study was to further test the Chest Pain Conception Questionnaire in a racially and ethnically diverse sample.

Methods
Participants from across the United States completed an online survey. Confirmatory factor analysis and descriptive statistics were used to characterize the instrument's performance.

Results
Participants (N = 597) were primarily women (59.6%), White (69.3%), and non-Hispanic (83.4%) with a mean age of 54.0 years (SD = 11.5). Confirmatory factor analysis supported the original 3-factor structure of the instrument, c
2(58) = 132.32, P = .000, root mean square error of approximation = 0.04, confirmatory fit index = 0.95, Tucker-Lewis index = 0.93, standardized root mean square residual = 0.04. Other instrument characteristics from this validation study were similar to the initial development study.

Conclusions
This study further supports construct validity and internal consistency of the instrument in the target population.
Diagnostic Accuracy of the ICD-10 Code for Lichen Sclerosus in Black Women
Authors: Akinshemoyin Vaughn Olushola L. , Anyanwu Precious A. , Estill Mariah C. 
Published: September 2024

Abstract
The prevalence of vulvar lichen sclerosus in Black women is unknown. There is a lack of validity using the International Statistical Classification of Diseases and Related Health Problems, Tenth Revision (ICD-10) code L90.0 to identify women with vulvar lichen sclerosus overall, with less accuracy in Black women.
Modulation of mechanosensation by endogenous dopaminergic signaling in the lateral parabrachial nucleus in mice
Authors: Koo Ho , Wang Jigong , Pariyar Ramesh , Hammond Regan M. , La Jun-Ho 
Published: September 2024

Abstract

Introduction:
The lateral parabrachial nucleus (LPBN), a crucial hub for integrating and modulating diverse sensory information, is known to express both D1 and D2 dopamine receptors and receive dopaminergic inputs. However, the role of the LPBN's dopaminergic system in somatosensory processing remains largely unexplored. In this study, we investigated whether mechanical sensory stimulation triggers dopamine release in the LPBN and how D1- and D2-like receptor signaling in the LPBN influences mechanosensitivity in mice.

Methods:
We used a G-protein-coupled receptor–based dopamine sensor to monitor dopamine release in the LPBN and a von Frey filament assay to measure the mechanical threshold for nocifensive withdrawal in mouse hind paws after unilateral microinjection of D1- or D2-like receptor antagonist into the LPBN.

Results:
Noxious mechanical stimulation increased the dopamine sensor signal in the LPBN. Thresholds of nocifensive withdrawal from mechanical stimulation were decreased by the D1-like receptor antagonist SCH-23390 (0.1 µg) but increased by the D2-like receptor antagonist eticlopride (1 µg). In the intraplantar capsaicin injection model that develops mechanical hypersensitivity in the injected paw, the dopamine sensor signal in the LPBN was increased, and eticlopride (1 µg) in the LPBN significantly inhibited the capsaicin-induced mechanical hypersensitivity.

Conclusions:
These results suggest that endogenous dopaminergic signaling occurs in the LPBN upon noxious mechanical stimulation, inhibiting mechanosensitivity through D1-like receptors while enhancing it through D2-like receptors. D2-like receptor signaling in the LPBN may contribute to an injury-induced increase in mechanical nociception, indicating that inhibiting the receptor within the LPBN could offer potential as a novel analgesic strategy.
Long-term Outcomes Following Colectomy and Liver Transplantation for Inflammatory Bowel Disease with Primary Sclerosing Cholangitis
Authors: Matar Abraham J. , Falconer Elissa , LaBella Michelle , Kapadia Muneera R. , Justiniano Carla F. , Olortegui Kinga Skowron , Steinhagen Randolph , Schultz Kurt , Pratap Anurag , Leeds Ira , Weaver Lauren , Gaertner Wolfgang , Finger Erik B. , Thompson Mary , Fair Lucas , Fichera Alessandro , Lovasik Brendan P. , Chapman William C. , McGeoch Catherine LB. , Camacho Mary Catherine , Kazimi Marwan , Kim Steven C. , Shaffer Virginia O. , Srinivasan Jahnavi K. 
Published: September 2024

Objective:
To investigate the long-term outcomes of patients with combined primary sclerosing cholangitis/inflammatory bowel disease (PSC-IBD) undergoing both liver transplantation (LT) and total abdominal colectomy (TAC).

Summary Background Data:
The fraction of patients with PSC-IBD that require both LT and TAC is small, thereby limiting significant conclusions regarding long-term outcomes.

Methods:
Adult and pediatric patients from nine centers from the US IBD Surgery Collaborative who underwent staged LT and TAC for PSC-IBD were included. Long-term outcomes, including survival, were assessed.

Results:
Among 127 patients, 66 underwent TAC-before-LT, with a median time from TAC to LT of 7.9 yrs, while 61 underwent LT-before-TAC, with a median time from LT to TAC of 4.4 years. Median patient survival post TAC was significantly worse in those undergoing LT-before-TAC (16.0 yrs vs. 42.6 yrs, P=0.007), while post LT survival was not impacted by the order of TAC and LT (21.6 yrs vs. 22.0 yrs, P=0.81). Patients undergoing TAC for medically refractory disease had a higher incidence of recurrent PSC (rPSC) (P=0.02) and biliary complications (0.09) compared to those undergoing TAC for oncologic indications. Definitive TAC reconstruction with either end ileostomy or ileal-pouch anal anastomosis (IPAA) did not impact post-LT or post-TAC outcomes.

Conclusions:
Long term survival in PSC-IBD was contingent upon progression to LT and was not impacted by the need for TAC. PSC-IBD patients undergoing TAC for medically refractory disease had a higher incidence of rPSC and biliary complications. The use of IPAA in PSC-IBD was a viable alternative to end ileostomy.
Determinants of household choice of cooking fuel in India: evidence from National Family Health Survey 5
Authors: Choudhury Dinesh Kumar , Guptha Siva Kiran , Gurung Rajiv 
Published: September 2024

PurposeMore than 40% of Indian households still rely on unclean cooking fuels, exposing particularly women and children to adverse health effects. This paper explores the factors determining a household’s cooking fuel choice in India.Design/methodology/approachThe study used the fifth round of the National Family Health Survey (2019-21) dataset. A chi-square test and a logistic regression were used for empirical analysis. Cooking fuel was categorized as “clean” and “unclean” cooking fuel.FindingsThe result shows that around 47% of the households are still using unclean cooking fuel in India. Households with a better-educated or female head, those with smaller family sizes, those with a higher level of wealth and those located in urban areas are more likely to use clean cooking fuel. Similarly, households headed by younger individuals and Muslim households are linked to more usage of clean cooking fuels. However, Scheduled Tribe (ST) and Other Backward Classes (OBC) households and those headed by older individuals are less likely to use clean cooking fuels.Research limitations/implicationsThe cross-sectional nature of data enables us to draw only associations between the variables and not causal relationships between them. The findings of this study have important implications for household energy policies in India and other developing nations. There is a need for policymakers to seriously consider socioeconomic factors in addressing issues and challenges associated with household energy consumption.Originality/valueThe study extends the empirical literature on determinants of household cooking fuel choice using the latest round of National Family Health Survey data from India.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-12-2023-0988
Destination brand gestalt: dimensionalizing co-created tourism destination branding
Authors: Mandagi Deske W. , Centeno Dave 
Published: September 2024

Purpose
Anchored in the theories of brand gestalt and stakeholder perspectives, this study aims to undertake a comprehensive examination of the brand gestalt concept, emphasizing its multidimensional nature and the process of co-creation.

Design/methodology/approach
Focused within the context of the Wonderful Indonesia brand, the research draws upon a rich qualitative data set derived from in-depth interviews conducted with 18 international tourists, supplemented by netnography (or internet ethnography) of websites, social media and online articles related to Wonderful Indonesia. Using grounded theory methodology, the qualitative data undergo rigorous analysis to identify emergent themes and patterns.

Findings
The research elucidates the four dimensions (4S) comprising brand gestalt: storyscapes, sensescapes, servicescapes and stakeholderscapes. Each dimension is further delineated into essential categories, providing a comprehensive understanding of brand gestalt. This study highlights the collaborative nature of brand gestalt, emphasizing the involvement of multiple stakeholders in shaping the brand's identity and perception. Consumer perceptions of co-creation are identified as significant contributors to brand gestalt, enhancing the brand's value proposition.

Practical implications
Destination management and practitioners can use the insights from the research to refine their brand management and marketing strategies by leveraging the dimensions of brand gestalt. Recognizing the collaborative construct of brand gestalt can guide businesses in fostering meaningful relationships with stakeholders and aligning branding efforts with collective visions. Understanding the role of consumer co-creation in brand development can inform strategies aimed at enhancing brand equity and fostering consumer loyalty.

Originality/value
This study extends existing literature on brand gestalt by providing a comprehensive examination of its four dimensions and essential categories. By emphasizing the collaborative nature of brand gestalt, this study contributes to advancing the understanding of brand co-creation paradigms. The identification of consumer perceptions of co-creation as a significant factor in brand gestalt adds novel insights to the literature, offering valuable implications for brand management and marketing strategies.
Flying to your home yard: the mediation and moderation model of the intention to employ drones for last-mile delivery
Authors: Ngah Abdul Hafaz , Thurasamy Ramayah , Rahi Samar , Kamalrulzaman Nurul Izni , Rashid Aamir , Long Fei 
Published: September 2024

PurposeDrones will become part of daily lives similar to smartphones becoming a staple of modern living. Nonetheless, only several past studies investigated the intention to utilise drones for parcel delivery however, the intention to use drones among online shoppers was not fully explored. The study attempts to investigate the factors influencing the intention to use drones for last-mile delivery.Design/methodology/approachA total of 292 data were gathered via an online survey among online shoppers applying a snowball sampling method. Since the study operationalised the measures as composites, a combination of reflective and formative measurement, and the study focusses on predictive purposes, partial least squares structural equation modelling with SmartPLS 4 was applied to test the model developed based on the stimulus-organism-response model.FindingsThe analysis found that all the direct hypotheses were found supported. Moreover, Green support, green desire and pro-environmental behaviour positively and sequentially mediated future orientation and intention, whereas technology anxiety and perceived safety moderated the relationship between pro-environmental behaviour and intention.Research limitations/implicationsThe respondents only limit to the online shoppers in Malaysia which based on purposive sampling method, thus the findings cannot be generalized to another countries.Practical implicationsBesides enriching the literature on drone studies, the findings provided practical insights to online platforms and drone operators to develop an effective strategy to encourage online shoppers to shift from conventional delivery to drone delivery.Originality/valueThe study developed a new model for drone delivery studies using the S-O-R model in introducing orientation towards the future and green support as the stimulus, green desire as an organism and pro-environmental behaviour and usage intention as a response. The study introduced multiple sequential mediators, also contributing to the S-O-R model to predict online shoppers' behaviour towards drones as a tool for last-mile delivery. Another important contribution, technology anxiety and perceived safety were confirmed to have a moderation effect for the relationship between pro-environmental behaviour and intention to use drones for last-mile delivery.