bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Electric Field Standing Wave Effect in Infrared Transmission Spectroscopy
Authors: Mayerhöfer Thomas G. , Mutschke Harald , Popp Jürgen 
Published: August 2017

AbstractWhen band ratios in infrared absorbance spectra of films are compared (which had been converted from transmittance spectra), it can be noted that even after background correction and removal of interference fringes these band ratios change with the thickness of the films. The main goal of this work is to show that this effect is a consequence of an electric field standing wave based on the coherent superposition of light waves in the film. We further investigate how transmittance and reflectance, as well as absorbance and the (from absorbance) regained index of absorption, depend on the thickness of the film and how these parameters influence the positions of bands. We compare the results with those for the incoherent case and the case where a single pass of light through the film without reflection loss is assumed.
Optimizing Dual Fluorescent Analysis to Investigate the Toxicity of AgNPs in E. coli
Authors: Hong Wei , Chen Shaopeng 
Published: August 2017

AbstractThe ever‐increasing use of silver nanoparticles (AgNPs) carries potential ecotoxicological risks. For full risk assessment, E. coli cells harboring a plasmid with a constitutively expressed GFP gene under control of lac promoter (lac::GFP) are extensively utilized. Flow cytometry is an advanced technology usually applied to toxicological research for rapid, efficient, multi‐parameter analysis of single cells. However, it is difficult to accurately and sensitively detect the toxicity of nanoparticles with flow cytometry due to the interference of aggregated nanoparticles. In this protocol, dual‐fluorescence detection with a propidium iodide (PI)–lac::GFP assay is used to determine the toxicity of AgNPs and successfully discriminate the dead or fragilized bacteria from living bacteria and aggregated nanoparticles. © 2017 by John Wiley & Sons, Inc.
Chemoenzymatic Synthesis of a Novel Borneol‐Based Polyester
Authors: Roth Steffen , Funk Irina , Hofer Michael , Sieber Volker 
Published: August 2017

AbstractTerpenes are a class of natural compounds that have recently moved into the focus as a bio‐based resource for chemical production, owing to their abundance, their mostly cyclic structures, and the presence of olefin or single hydroxy groups. To apply this raw material in new industrial fields, a second hydroxy group is inserted into borneol by cytochrome P450cam (CYP101) enzymes in a whole‐cell catalytic biotransformation with Pseudomonas putida KT2440. Next, a semi‐continuous batch system was developed to produce 5‐exo‐hydroxyborneol with a final concentration of 0.54 g L−1. The bifunctional terpene was then used for the synthesis of a bio‐based polyester by a solvent‐free polycondensation reaction. The resulting polymer showed a glass transition temperature of around 70 °C and a molecular weight in the range of 2000–4000 g mol−1 (Mw). These results show that whole‐cell catalytic biotransformation of terpenes could lead to bio‐based, higher‐functionalized monomers, which might be basic raw materials for different fields of application, such as biopolymers.
Graphene‐Supported Pyrene‐Modified Cobalt Corrole with Axial Triphenylphosphine for Enhanced Hydrogen Evolution in pH 0–14 Aqueous Solutions
Authors: Li Xialiang , Lei Haitao , Guo Xiaojun , Zhao Xueli , Ding Shuping , Gao Xueqing , Zhang Wei , Cao Rui 
Published: August 2017

AbstractA cobalt complex of 5,15‐bis(pentafluorophenyl)‐10‐(4)‐(1‐pyrenyl)phenyl corrole that contains a triphenylphosphine axial ligand (1‐PPh3) was synthesized and examined as an electrocatalyst for the hydrogen evolution reaction (HER). If supported on graphene (G), the resulting 1‐PPh3/G material can catalyze the HER in aqueous solutions over a wide pH range of 0–14 with a high efficiency and durability. The significantly enhanced activity of 1‐PPh3/G, compared with that of its analogues 1‐py/G (the Co‐bound axial ligand is pyridine instead of triphenylphosphine) and 2‐py/G (Co complex of 5,10,15‐tris(pentafluorophenyl)corrole), highlights the effects of the pyrenyl substituent and the triphenylphosphine axial ligand on the HER activity. On one hand, the pyrenyl moiety can increase the π–π interactions between 1 and graphene and thus lead to a fast electron transfer from the electrode to 1. On the other hand, the triphenylphosphine axial ligand can increase the electron density (basicity) of Co and thus make the metal center more reactive to protons at the trans position through a so‐called “push effect”. This study concerns a significant example that shows the trans effect of the axial ligand on the HER, which has been investigated rarely. The combination of various ligand‐design strategies in one molecule has been realized in 1‐PPh3 to achieve a high catalytic HER performance. These factors are valuable to be used in other molecular catalyst systems.
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO2‐acidified sea water
Authors: Bailey Allison , De Wit Pierre , Thor Peter , Browman Howard I. , Bjelland Reidun , Shema Steven , Fields David M. , Runge Jeffrey A. , Thompson Cameron , Hop Haakon 
Published: August 2017

AbstractOcean acidification is the increase in seawater pCO2 due to the uptake of atmospheric anthropogenic CO2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO2, and associated decrease in pH, represents a climate change‐related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal‐level performance (development, growth, respiration) of C. glacialis nauplii is unaffected by low pH. Here, we investigated the molecular‐level response to lowered pH in order to elucidate the physiological processes involved in this tolerance. Nauplii from wild‐caught C. glacialis were cultured at four pH levels (8.05, 7.9, 7.7, 7.5). At stage N6, mRNA was extracted and sequenced using RNA‐seq. The physiological functionality of the proteins identified was categorized using Gene Ontology and KEGG pathways. We found that the expression of 151 contigs varied significantly with pH on a continuous scale (93% downregulated with decreasing pH). Gene set enrichment analysis revealed that, of the processes downregulated, many were components of the universal cellular stress response, including DNA repair, redox regulation, protein folding, and proteolysis. Sodium:proton antiporters were among the processes significantly upregulated, indicating that these ion pumps were involved in maintaining cellular pH homeostasis. C. glacialis significantly alters its gene expression at low pH, although they maintain normal larval development. Understanding what confers tolerance to some species will support our ability to predict the effects of future ocean acidification on marine organisms.
Using streamflow observations to estimate the impact of hydrological regimes and anthropogenic water use on European stream macroinvertebrate occurrences
Authors: Domisch Sami , Portmann Felix T. , Kuemmerlen Mathias , O'Hara Robert B. , Johnson Richard K. , Davy‐Bowker John , Bækken Torleif , Zamora‐Muñoz Carmen , Sáinz‐Bariáin Marta , Bonada Núria , Haase Peter , Döll Petra , Jähnig Sonja C. 
Published: August 2017

AbstractUnderstanding the drivers of stream macroinvertebrate distribution patterns—the most diverse animal group in freshwater ecosystems—is a major goal in freshwater biogeography. Climate and topography have been shown to explain species' distributions at continental scales, but the contribution of natural and anthropogenically altered streamflow is often omitted in large‐scale analyses due to the lack of appropriate data. We test how macroinvertebrate occurrences can be linked to streamflow observations and evaluate the relative importance of streamflow regimes and water use for macroinvertebrate occurrences from 19 orders across Europe. We first paired species sampling locations with hydrological gauging stations considering 5 combinations of the geographic distance and difference in flow accumulation (upstream contributing area). We then used Generalized Linear Models to assess the influence of the streamflow regime, simulated water use, and climate and topography on the occurrence of macroinvertebrates. The pairing method that assigned species records to the closest gauging station in terms of both distance and flow accumulation performed best. Most of the species studied occurred preferentially in river habitats with low mean annual streamflow and streamflow variability, high winter streamflow, and low levels of water withdrawals for irrigation or manufacturing. We conclude that flow accumulation is a useful proxy to evaluate the proximity of species records to gauging stations, omitting species records that do not belong to a given stream reach. The strong contribution of streamflow and water use indicators on macroinvertebrate occurrences underline their importance for yielding robust occurrence estimates.
The effect of lizards on spiders and wasps: variation with island size and marine subsidy
Authors: Piovia‐Scott Jonah , Yang Louie H. , Wright Amber N. , Spiller David A. , Schoener Thomas W. 
Published: August 2017

AbstractIntroduced predators can have dramatic effects on island ecosystems, the magnitude of which are likely to vary with island characteristics. We investigated the influence of two important properties of islands—size and amount of resource subsidy—on the effects of an introduced predatory lizard (Anolis sagrei) on three groups of arthropod prey. Lizards were experimentally introduced to 16 islands that spanned gradients in vegetated area and seaweed deposition (a marine resource subsidy); 16 similar islands served as lizard‐free controls. The abundance of web spiders, salticid spiders, and wasps was estimated prior to lizard introduction and again four months after lizard introduction. Lizard introduction reduced the average abundance of all three groups of arthropods. The effect of lizards on salticid spiders—which was very large (94% reduction in salticid abundance)—decreased with island size. In contrast, the effect of lizards on wasps—which was also very large (88% reduction in wasp abundance)—tended to increase with island size, but with only marginal significance. There was no evidence for variation in the effect of lizards on web spiders with island size. This variation between prey taxa may be related to the relative importance of environmental stress (such as wind and wave exposure, which tend to be more pronounced on smaller islands) in determining abundance. Salticids seem to tolerate the stressful environmental conditions that characterize smaller islands, allowing for larger lizard effects; wasps seem to be limited by these conditions (either directly or indirectly via reduced prey availability), minimizing lizard effects on smaller islands. There was a marginally significant tendency for the effect of lizards on salticid spiders to be weaker on islands with more seaweed deposition, suggesting that subsidies may play a role in reducing predator effects on islands. Our results highlight the importance of ecological context in determining the top‐down effects of introduced predators and underscore the need to extend existing theories relating island area and community characteristics toward an explicit consideration of species interactions.
Herpes simplex virus 1 interferes with autophagy of murine dendritic cells and impairs their ability to stimulate CD8+ T lymphocytes
Authors: Budida Ramachandramouli , Stankov Metodi V. , Döhner Katinka , Buch Anna , Panayotova‐Dimitrova Diana , Tappe Kim A. , Pohlmann Anja , Sodeik Beate , Behrens Georg M. N. 
Published: August 2017

AbstractThe MHC class I presentation is responsible for the presentation of viral proteins to CD8+ T lymphocytes and mainly depends on the classical antigen processing pathway. Recently, a second pathway involving autophagy has been implicated in this process. Here, we show an increase in the capacity of murine dendritic cells (DCs) to present viral antigens on MHC class I after infection with a mutant herpes simplex virus 1 (HSV‐1‐Δ34.5), lacking infected cell protein 34.5 (ICP34.5), when compared to its parental HSV‐1 strain. The ICP34.5 protein counteracts host cell translational arrest and suppresses macroautophagy, and the lack of this protein resulted in a low viral protein abundance, which was processed and presented in an efficient way. Our study demonstrates an important role of autophagy in processing endogenous viral proteins in HSV‐1‐infected DCs.
Short certificate‐based proxy signature scheme from pairings
Authors: Verma Girraj Kumar , Singh B. B. 
Published: August 2017

AbstractRecently, the delegation of signing capacity has become a common practice to develop applications like distributed computing, grid computing, mobile agents, mobile communication, etc. The method of proxy signing, introduced by Mambo et al, is the tool of such delegation. In 2003, Gentry introduced the paradigm of certificate‐based encryption to remove the key escrow problem in identity‐based public key cryptography (PKC) and to combine the merits of PKC and identity‐based PKC. This article presents an efficient and short provably secure certificate‐based proxy signature scheme from pairing over elliptic curves. The aim is to devise a method of delegation with less space and low‐bandwidth requirement. Through efficiency comparison with existing efficient proxy signature schemes, we show that our scheme is the most appealing for low‐bandwidth communication channels applied for wireless communication, online transactions, e‐bookings, mobile agents, etc.
FarmaREL: An Italian pharmacovigilance project to monitor and evaluate adverse drug reactions in haematologic patients
Authors: Fracchiolla Nicola S. , Artuso Silvia , Cortelezzi Agostino , Pelizzari Anna M. , Tozzi Paola , Bonfichi Maurizio , Bocchio Federica , Gargantini Livio , De Rosa Elisa , Vighi Giuseppe D. , Prestini Lucia , Sammassimo Simona , Frungillo Niccolò , Pasquini Maria C. , Ragazzi Alessandra , Boghi Daniele , Pastore Alessia , Lanzi Eraldo , Gritti Giuseppe , Quaresmini Giulia , Voltolini Simone , Gaiardoni Roberta , Corti Consuelo , Vilardo Maria C. , La Targia Maria L. , Berini Giacomo , Magagnoli Massimo , Bacci Claudia , Consonni Dario , Rivolta Alma L. , Muti Giuliana 
Published: August 2017

AbstractAdverse drug reactions (ADRs) reduce patients' quality of life, increase mortality and morbidity, and have a negative economic impact on healthcare systems. Nevertheless, the importance of ADR reporting is often underestimated. The project “FarmaREL” has been developed to monitor and evaluate ADRs in haematological patients and to increase pharmacovigilance culture among haematology specialists. In 13 haematology units, based in Lombardy, Italy, a dedicated specialist with the task of encouraging ADRs reporting and sensitizing healthcare professionals to pharmacovigilance has been assigned. The ADRs occurring in haematological patients were collected electronically and then analysed with multiple logistic regression. Between January 2009 and December 2011, 887 reports were collected. The number of ADRs was higher in older adults (528; 59%), in male (490; 55%), and in non‐Hodgkin lymphoma patients (343; 39%). Most reactions were severe (45% required or prolonged hospitalization), but in most cases, they were fully resolved at the time of reporting. According to Schumock and Thornton criteria, a percentage of ADRs as high as 7% was found to be preventable versus 2% according to reporter opinion. Patients' haematological diagnosis, not age or gender, resulted to be the variable that most influenced ADR, in particular severity and outcome. The employment of personnel specifically dedicated to pharmacovigilance is a successful strategy to improve the number and quality of ADR reports. “FarmaREL”, the first programme of active pharmacovigilance in oncohaematologic patients, significantly contributed to reach the WHO “Gold Standard” for pharmacovigilance in Lombardy, Italy.
Effect of follicular diameter at the time of ovulation triggering on pregnancy outcomes during intrauterine insemination
Authors: Maher Mohammad A. , Abdelaziz Ahmed , Shehata Yasser A. 
Published: August 2017

AbstractObjectiveTo compare pregnancy outcomes when triggering ovulation at different follicle sizes during intrauterine insemination (IUI) cycles.MethodsA prospective observational study was undertaken at two collaborative fertility centers in Saudi Arabia between January 2014 and May 2016. Women of any age were enrolled if they met inclusion criteria: primary, secondary, or unexplained infertility (≥1 year); day‐2 follicle‐stimulating hormone less than 12 IU/mL; normal prolactin, thyroid function, and uterine cavity; at least one patent tube; and a male partner with normal semen count and motility. IUI cycles were subdivided by size of dominant follicle (17 to <18 mm, 18 to <19 mm, 19 to <20 mm, and ≥20 mm), and pregnancy outcomes compared.ResultsData from 516 IUI cycles were analyzed. Frequencies of clinical pregnancy, ongoing pregnancy, and live birth for a follicle size of 19–20 mm were 30.2% (39/129), 24.0% (31/129), and 24.0% (31/129), respectively; these rates were significantly higher than those in other groups (all P<0.05). Only endometrial thickness was found to also contribute to outcome: probability of pregnancy increased as thickness rose (odds ratio 1.148, 95% confidence interval 1.065–1.237; P<0.001).ConclusionThe optimal follicular diameter associated with increased pregnancy rates in gonadotropin‐stimulated IUI cycles was between 19 and 20 mm.
Curcumin primed exosomes reverses LPS‐induced pro‐inflammatory gene expression in buffalo granulosa cells
Authors: Vashisht Monika , Rani Payal , Sunita  , Onteru Suneel Kumar , Singh Dheer 
Published: August 2017

AbstractCurcumin possesses anti‐inflammatory properties and provides a promising treatment for inflammation. The aim of the study is to establish that buffalo granulosa cells when primed with curcumin (20 μM), release improved cellular contents through exosome that can mitigate granulosa cell dysfunction. Recently, we have shown that buffalo granulosa cells exposed to LPS (1 μg/mL) in serum free culture, transiently increased the pro‐inflammatory cytokine genes (IL‐1β, TNF‐α, IL‐6) expression followed by the inhibition of CYP19A1 gene expression and estradiol production. Therefore, LPS‐treated granulosa cells were used as a model of inflammation and curcumin primed exosomes were utilized to check their potential for reducing granulosa cell dysfunction. Expression level of pro‐inflammatory cytokines and CYP19A1 were detected by real time PCR while estradiol levels were measured by ELISA. Exosomes derived from curcumin‐treated cells alleviated LPS mediated inflammation. In conclusion, our study potentiates the use of curcumin primed exosomes in mitigating granulosa cell dysfunction. Results show the therapeutic conservatories of curcumin via primed exosomes.
VEGFB‐VEGFR1 ameliorates Ang II‐induced cardiomyocyte hypertrophy through Ca2+‐mediated PKG I pathway
Authors: Shen Zhijie , Zhang Zhihui , Wang Xiaoyan , Yang Kan 
Published: August 2017

AbstractIn response to assorted stimuli, the heart will develop into cardiomyocyte hypertrophy, but sustained cardiomyocyte hypertrophy will finally lead to heart failure. This research is aimed to examine the effect of VEGFB on cardiomyocyte hypertrophy by using the cardiomyocyte‐derived cell line H9C2 of cultured rates. It turns out that VEGFB can positively prevent the Ang II‐induced rising in the size of cardiomyocyte as well as reduce Ang II‐induced mRNA and protein levels of β‐MHC (β‐myosin heavy chain), BNP (brain natriuretic peptide), and ANP (atrial natriuretic peptide). Moreover, VEGFB can regulate the decline of the Ang II‐induced rising in Ca2+. After VEGFR1 knockdown, these effects of VEGFB were partially reversed. Moreover, VEGFB attenuated the suppression of PKG I, p‐VASP, and RGS2 caused by Ang II; whereas VEGFR1 knockdown partially abolished the indicated effect of VEGFB. In a word, the effect of VEGFB on relevant downstream targets and the pathways of PKG I by VEGFR1 may explain its efficacy on cardiomyocyte hypertrophy. Thus, it can be suggested that it is feasible to apply VEGFB‐VEGFR1 for reducing the symptoms of cardiomyocyte hypertrophy.
Biostimulants from food processing by‐products: agronomic, quality and metabolic impacts on organic tomato (Solanum lycopersicum L.)
Authors: Abou Chehade Lara , Al Chami Ziad , De Pascali Sandra Angelica , Cavoski Ivana , Fanizzi Francesco Paolo 
Published: August 2017

AbstractBACKGROUNDBiostimulants have recently gained increased attention due to their multiple benefits for sustainable agriculture. In this study, three food processing by‐products – fennel processing residues (FPR), lemon processing residues (LPR) and brewer's spent grain (BSG) – were investigated as potential sources of biostimulants. Their aqueous extracts as individual and associated applications were assessed for their effects on agronomic, quality and metabolic performance of organic tomato in comparison to extract of humic substances (HS) and untreated control (CTRL).RESULTSOnly FPR extracts stimulated shoot growth and tomato dry matter content, whereas all candidates improved tomato yield. FPR and BSG increased fruit mineral content and BSG‐FPR‐LPR in combination enhanced titratable acidity. FPR‐treated fruits had also 20% more vitamin C than CTRL, and higher phenol content was obtained in those of BSG‐LPR. Fruit metabolomic profile showed the tendency of all extracts, except BSG‐LPR, to increase tomato citric acid and to decrease β‐glucose and methanol concentrations. The analysis revealed accordingly the indispensable role of FPR in combined applications for inducing an HS‐like response in fruits.CONCLUSIONThe results were indicative of the biostimulant activity of these extracts and demonstrated them, particularly FPR, as promising candidates for enhancing plant productivity and fruit quality. © 2017 Society of Chemical Industry