bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo human adipose-derived mesenchymal stem cell tracking after intra-articular delivery in a rat osteoarthritis model
Authors: Li Meng , Luo Xuan , Lv Xiaoteng , Liu Victor , Zhao Guangyu , Zhang Xue , Cao Wei , Wang Richard , Wang Wen 
Published: November 2016

Abstract
Background
Human adipose-derived mesenchymal stem cells (haMSCs) have shown efficacy in treating osteoarthritis (OA) both preclinically and clinically via intra-articular (IA) injection. However, understanding the mode of action of the cell therapy has been limited by cell tracking capability and correlation between the pharmacokinetics of the injected cells and the intended pharmacodynamics effect. This study aims to explore methodology and to understand in vivo biodistribution of clinical-grade haMSCs labeled with fluorescent dye and injected into an immunocompetent OA rat model.

Methods
haMSCs labeled with fluorescent dye were investigated for their proliferation and differentiation capabilities. Labeled cells were used to establish detection threshold of a noninvasive biofluorescent imaging system before the cells (2.5 × 106) were injected into a conventional rat OA model induced by medial meniscectomy for 8 weeks. We attempted to reveal the existence of labeled cells in vivo by imaging and a molecular biomarker approach, and to correlate with the in vivo efficacy and physical presence over a follow-up period up to 10 weeks.

Results
In vitro proliferation and differentiation of haMSCs were not affected by the labeling of DiD dye. Detection thresholds of the labeled cells in vitro and in vivo were determined to be 104 and 105 cells, respectively. When 2.5 × 106 haMSCs were injected into the joints of a rat OA model, fluorescent signals (or >105 cells) lasted for about 10 weeks in the surgical knee joint at the same time as efficacy was observed. Signals in nonsurgical rats only lasted for 4 weeks. The human MSCs were shown to engraft to the rat joint tissues and were proliferative. Human FOXP2 gene was only detected in the knee joint tissue, suggesting limited biodistribution locally to the joints.

Conclusions
The current study represents the first attempt to correlate cell therapy efficacy on OA with the physical presence of the injected haMSCs in the OA model, and demonstrates that human adipose-derived mesenchymal stem cells persisted for 10 weeks locally in the rat joint, coinciding with the efficacy observed. It is postulated that persistence and/or proliferation of the haMSCs in the joint is required in order to exert their functions on promoting joint regeneration and/or cartilage protection, further supporting the safety and feasibility of IA injection of MSCs for the treatment of OA patients.
Comparison of Supraclavicular and Infraclavicular Brachial Plexus Block: A Systemic Review of Randomized Controlled Trials
Authors: Park Sun-Kyung , Lee Su-Young , Kim Won Ho , Park Han-Seul , Lim Young-Jin , Bahk Jae-Hyon 
Published: November 2016

BACKGROUND:
Supraclavicular (SC) and infraclavicular (IC) brachial plexus block (BPB) are commonly used for upper extremity surgery. Recent clinical studies have compared the effect of SC- and IC-BPB, but there have been controversies over spread of sensory blockade in each of the 4 peripheral nerve branches of brachial plexus.

METHODS:
This study included a systemic review, using the Medline and EMBASE database from their inceptions through March 2016. Randomized controlled trials (RCTs) comparing SC- and IC-BPB were included. The prespecified primary outcome was the incidences of incomplete sensory blockade in each of the 4 terminal nerve branches of brachial plexus. Secondary outcome included the incidence of successful blockade, performance time, onset of sensory block, duration of analgesia, and complication rates.

RESULTS:
Ten RCTs involving 676 patients were included. Pooled analyses showed the incidence of incomplete block at 30 minutes in radial nerve territory was significantly higher in IC-BPB, favoring SC-BPB (risk ratio 0.39; 95% confidence interval [0.17–0.88], P = .02, I
2 = 0%). However, subgroup analysis according to the number of injections of IC-BPB showed that double or triple injections IC-BPB yielded no difference in the incomplete radial block. Furthermore, the incidence of incomplete ulnar block at 30 minutes was significantly lower in IC-BPB when using double or triple injection IC-BPB. There was no difference in the secondary outcomes between SC- and IC-BPB groups, with the exception of complication rates. The incidence of paresthesia/pain on local anesthetic injection, phrenic nerve palsy, and Horner syndrome was significantly higher in the SC group, favoring IC-BPB.

CONCLUSIONS:
This meta-analysis demonstrated that IC-BPB showed a significantly high incidence of incomplete radial nerve sensory block at 30 minutes, which may be avoided by double or triple injection. Furthermore, IC-BPB with multiple injection technique showed significantly lower incidence of incomplete ulnar block than SC-BPB. There were no differences in the incidence of successful blockade, block onset, and duration of analgesia between SC- and IC-BPB. Procedure-related paresthesia/pain and adjacent nerve-related complications were more frequent in SC-BPB. However, because of the small sample size, publication bias remains a concern when interpreting our results. Further studies with sufficient sample size and reporting large number of outcomes are required.
CUSTOMER INTELLECTUAL EQUITY IN SERVICES IMPROVEMENT SYSTEM
Authors: Дьяченко Александр , Dyachenko Aleksandr 
Published: November 2016

The article presents results of the theoretical research of the customer equity. In accordance with them the corresponding concept is synthesized. The customer capital is identified as a system of stable and mutually beneficial relations of economic agents, buyers, consumers and staff of the enterprise. Such relations form the structure of the effective cooperation, which con- tributes to gaining by companies additional values. It is provides the customers’ repeated requests and attracting new ones, as well as increases competitive advantage and companies’ capitalization. We revealed the necessity of finding new forms of the customer equity, containing the elements of art, which will contribute to proposal of services, about which the consumers dream. The author based on the research result formulates the concept of use value of art, which induces to crave their presence again and again. An indicator of the art element use value is the value of the additional profits obtained due to the talented artistic expression. The customer equity is expedient to improve, focusing on the implementation of the popular theatrical services, ensuring their effective promotion using aggressive methods of direct marketing and personal sellings. Differentiation of the client capital services art use value should be made taking into account the interests, tastes, preferences of target customers, and their valuable relation to a particular style of art. Product differentiation by the elements of art inspires people&#180;s lives. Talent, kindness, spirituality of senses, thoughts, and actions of the artist should be directed to the use of beauty flashes happening in the life for its meaningful creative heyday in the industrial production.
The work was supported by the Russian Foundation for Humanities Grant №16-12-34504.
Clinician and client views of utilising early supported discharge services
Authors: Kraut Jacey , Singer Barbara J , Singe Kevin P 
Published: November 2016

Background/Aims:To explore the beliefs and attitudes of potential referrers and referrees regarding the possible utilisation of early supported discharge (ESD) prior to hospital discharge.Methods:Semi-standardised one-to-one interviews were conducted with nine consultants and ten dyads, comprising inpatients and corresponding treating staff members involved in referral of these patients to ESD. Content analysis was completed to identify key themes and to group the data into categories.Results:Rehabilitation consultants reported safety and the ability to manage at home were the primary considerations in ESD referral decision making. Most patients were extremely likely to agree to referral to ESD. Four main categories were identified in the responses from the patient/staff dyads, which were summarised as: positive and negative aspects of ESD, and barriers and enablers to early discharge. Patients were only willing to return home with ESD once they were ambulant in some capacity. Staff identified considerably more potential disadvantages, and fewer benefits than patients with regard to early discharge. However, there was no indication that staff beliefs had a negative influence on patient views regarding their participation in ESD.Conclusions:Patients and staff generally approved of ESD services; however, staff may need to place greater consideration on advantages, such as the potential emotional and mental benefits that some patients associate with returning home sooner with ESD.
The effect of aerobic exercise on dual-task gait in individuals with Parkinson's disease
Authors: Swank Chad , Medley Ann , Thompson Mary , Trudelle-Jackson Elaine , Barisa Mark 
Published: November 2016

Background/Aims:Dual tasking exacerbates gait deficits in people with Parkinson's disease. This pilot study aimed to examine the effect of aerobic exercise on dual-task gait in individuals with Parkinson's disease and begin to identify the impact of volitional pedal rate.Methods:Twenty people with Parkinson's disease were recruited and tested at baseline, post-intervention, and follow-up for motor (Unified Parkinson's Disease Rating Scale) and cognitive function (Repeatable Battery for the Assessment of Neuropsychological Status) and dual-task gait (Time Up and Go) conditions (manual and cognitive). A 12-week aerobic exercise intervention of cycle ergometry was carried out three times a week for 60 minutes each session. To assess our intervention, separate repeated measures of analysis of variance were conducted. As determined a priori, to assess the impact of volitional pedal rate, participants were divided after the intervention into a high-pedal rate group (>60 rpm) and a low-pedal rate group (≤60 rpm) based upon average maximal pedal rate throughout the intervention and analysed with the Mann Whitney U test and Related Samples Wilcoxon Signed Rank Test.Findings:Overall, motor function (P=0.580), cognitive function (P=0.077), and motor-cognitive interplay (P=0.168) did not improve after aerobic exercise. However, pedal rate groups were different for TUGmanual and TUGcognitive, but not on motor or cognitive function. Only low-pedal rate group improved over time (P=0.028 on TUGmanual) and demonstrated reduced dual task cost after the aerobic exercise (>Minimal Detectible Change of 18.1%).Conclusions:Though the low-pedal rate group experienced greater motor-cognitive interference, persons who pedalled at the slower rate demonstrated the most improvement in dual-task activities.
Plasma rich in growth factors promotes dermal fibroblast proliferation, migration and biosynthetic activity
Authors: Anitua E. , Pino A. , Orive G. 
Published: November 2016

Objective: The use of plasma rich in growth factors (PRGF) has gained importance in many medical fields due to its regenerative potential. The aim of this study is to evaluate the effects of PRGF on primary skin fibroblasts assessing cell proliferation, migration and secretion of growth factors. The age of the patients from who PRGF was prepared was also studied to determine whether it influenced the outcomes. Method: Human dermal fibroblasts were isolated from three healthy volunteers. Using PRGF-Endoret technology, PRGF was prepared from two groups of different ages (18–35 years and 50+ years). The effects of increasing concentration of PRGF (5%, 10% and 20%) on cell proliferation and migration was evaluated. Biosynthetic behaviour of cells was also analysed measuring vascular endothelial growth factor (VEGF), transforming growth factor b1 (TGFb1) and pro-collagen type I secreted levels with or without PRGF treatment. Results: Mean platelet enrichment reached 2.4X and 2X in 18–35 and 50+ groups respectively. A dose-dependent response was observed in proliferation assays achieving the highest levels with 20% PRGF. Migration was also promoted in cells but not in a dose-dependent manner. Cell proliferation and migration outcomes obtained with PRGF (from both groups) were significantly higher compared to non-stimulated groups (p<0.05), with no statistical significances were observed between the different age groups. Production of VEGF, TGFb and procollagen type I was significantly increased by cells treated with PRGF, however, with the exception of VEGF, no statistical significances were observed between the different age groups. Conclusion: Results from this study concluded that PRGF is safe and effective in stimulating skin regeneration by enhancing proliferation, migration and expression of pivotal bioactive molecules involved in wound healing and haemostasis.
Unraveling the Molecular Complexity of O-Glycosylated Endogenous (N-Terminal) pro–B-Type Natriuretic Peptide Forms in Blood Plasma of Patients with Severe Heart Failure
Authors: Halfinger Bernhard , Hammerer-Lercher Angelika , Amplatz Benno , Sarg Bettina , Kremser Leopold , Lindner Herbert H 
Published: November 2016

Abstract

BACKGROUND
Currently, N-terminal pro–B-type natriuretic peptide (NT-proBNP) and its physiologically active counterpart, BNP, are most frequently used as biomarkers for diagnosis, prognosis, and disease monitoring of heart failure (HF). Commercial NT-proBNP and BNP immunoassays cross-react to varying degrees with unprocessed proBNP, which is also found in the circulation. ProBNP processing and immunoassay response are related to O-linked glycosylation of NT-proBNP and proBNP. There is a clear and urgent need to identify the glycosylation sites in the endogenously circulating peptides requested by the community to gain further insights into the different naturally occurring forms.

METHODS
The glycosylation sites of (NT-) proBNP (NT-proBNP and/or proBNP) were characterized in leftovers of heparinized plasma samples of severe HF patients (NT-proBNP: &gt;10000 ng/L) by using tandem immunoaffinity purification, sequential exoglycosidase treatment for glycan trimming, β-elimination and Michael addition chemistry, as well as high-resolution nano-flow liquid chromatography electrospray multistage mass spectrometry.

RESULTS
We describe 9 distinct glycosylation sites on circulating (NT-) proBNP in HF patients. Differentially glycosylated variants were detected based on highly accurate mass determination and multistage mass spectrometry. Remarkably, for each of the identified proteolytic glycopeptides, a nonglycosylated form also was detectable.

CONCLUSIONS
Our results directly demonstrate for the first time a rather complex distribution of the endogenously circulating glycoforms by mass spectrometric analysis in HF patients, and show 9 glycosites in human (NT-) proBNP. This information may also have an impact on commercial immunoassays applying antibodies specific for the central region of (NT-) proBNP, which detect mostly nonglycosylated forms.
Utilisation of CFBC Fly Ash in Hardening Slurries for Flood-Protecting Dikes
Authors: Kledyński Z. , Falaciński P. , Machowska A. , Dyczek J. , Kotwica Ł. 
Published: November 2016

Abstract
Strength and permeability properties along with microstructural evolution of hardened slurries composed of fly ash from fluidal bed combustion of brown coal and an addition of OPC/BFSC is assessed in this paper. An increase in the amount of fly ash in slurries influences the development of mechanical strength and a decrease of hydraulic conductivity. SEM, XRD, and porosity analyses confirmed formation of watertight microstructures. The structure of slurries is composed of ettringite, C-S-H phase, AFt, and AFm phases. Ettringite crystallises as relatively short needles forming compact clusters or intermixed with the C-S-H phase. The occurring C-S-H phases are mainly of type I - fibrous and type II - honeycomb.
Effects of a 6-Month Conditioning Program on Motor and Sport Performance in The Group of Children’s Fitness Competitors
Authors: Mlsnová Gabriela 
Published: November 2016

Summary
The aim of our study was to determine changes in sport and motor performance of competitors in the category of children’s fitness as a result of conditioning training intervention. We conducted a two-group simultaneous experiment. Experimental group (EG) and control group (CG) consisted of 18 girls competing in the 12 to 15 years old age categories. EG performed supervised conditioning program over a period of 25 weeks with training frequency 3 times per week. Based on the results of physical tests, competitive and expert assessments of sport performance in the children’s fitness category we found significant effect of our conditioning program to increase sport and motor performance in the experimental group. Subsequently, these improvements could lead to success in domestic and international competitions where they occupied the leading positions. Significant relationships (EG = 19; CG = 10) were found between competitive and expert assessments as well as physical tests results, between expert and competitive assessments of physiques and routines. These changes manifested positively not only in the competitive assessment of the physique but also in the expert “blind“ assessment in the competitive discipline of the physique presentation in quarter turns where we observed significant improvements in the EG. Based on the obtained results we recommend to increase the ratio of conditioning training to gymnastic-dance training to 50 %, inclusion of strengthening and plyometric exercises into the training process and monitor regularly the level of general and specific abilities of the competitors in the individual mezocycles of the annual training cycle.
Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets
Authors: Guran Husnu Sahan , Vural Aydın , Erkan Mehmet Emin , Durmusoglu Halil 
Published: November 2016

Abstract
Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliCH7, eaeA, stx1, stx2 and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbEO157 and eaeA genes; but not any fliCH7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx1 or stx2 genes, which were related to important virulence factors of the disease.
Dietary supplementation of probiotics in poultry exposed to heat stress – a review
Authors: Sugiharto Sugiharto , Yudiarti Turrini , Isroli Isroli , Widiastuti Endang , Kusumanti Endang 
Published: November 2016

Abstract
Heat-related stress has become a serious problem in poultry industry along with the global temperatures rise. Heat stress causes detrimental effects on physiology, immunology and microbiology resulting in abnormalities and impaired performances of birds. Several nutritional strategies have been conducted to counteract the detrimental effects of heat stress in poultry, including dietary supplementation of probiotics. This strategy has been proposed to ameliorate the intestinal ecosystem, physiological conditions and immune system, leading to the improved performance and health of birds subjected to heat stress. This review presents the potential benefits of probiotics against heat stress in poultry from the viewpoint of intestinal microbial ecology, morphology and structure, physiological conditions, immune system and production performances. The possible mechanisms through which probiotics may give beneficial impacts on heat-stressed birds are also discussed along with the data reporting the possible drawbacks of using probiotics in heat-stressed poultry.
Modeling of imaging fiber bundles and adapted signal processing for fringe projection
Authors: Matthias Steffen , Kästner Markus , Reithmeier Eduard 
Published: November 2016

Abstract
Fringe projection profilometry is an established technique for capturing three-dimensional (3-D)-geometry data with high-point densities in short time. By combining fringe projection with endoscopy techniques, it is possible to perform inline inspection of industrial manufacturing processes. A new fringe projection system is presented, which uses flexible image fiber bundles to achieve versatile positioning of a compact sensor head. When measuring specimens with highly varying reflectivity, such as technical surfaces on tool geometries, measurement errors increase especially due to the crosstalk between individual fibers in the bundle. A detailed analysis of the transmission properties of the utilized fiber bundles is presented. It is shown that aliasing is avoided due to the non-regular grid structure of a bundle. Different techniques are demonstrated to reduce the effect of crosstalk on the phase evaluation. Measurements of highly reflective technical surfaces with different geometrical properties are shown.
Abstraction and Diagrammatic Reasoning in Aristotle’s Philosophy of Geometry
Authors: Humphreys Justin 
Published: November 2016

AbstractAristotle’s philosophy of geometry is widely interpreted as a reaction against a Platonic realist conception of mathematics. Here I argue to the contrary that Aristotle is concerned primarily with the methodological question of how universal inferences are warranted by particular geometrical constructions. His answer hinges on the concept of abstraction, an operation of “taking away” certain features of material particulars that makes perspicuous universal relations among magnitudes. On my reading, abstraction is a diagrammatic procedure for Aristotle, and it is through imagined variation of diagrams that one can universalize spatial inferences. In order to shift the discussion of Aristotle’s work on geometry from ontological to methodological questions, I argue in the first section that geometrical properties for Aristotle are necessary accidents of the particulars, canonically diagrams, in which they inhere. I then consider a line of research that shows how diagrams in ancient geometry are not illustrations of the textual proof but are in part constitutive of geometrical inference, an insight I claim can shed light on Aristotle’s philosophical project. Next I substantiate this assertion by giving an alternative interpretation of abstraction according to which it is a diagrammatic procedure that allows a part of a particular diagram to stand in for a universal. In the last section, I consider Aristotle’s account of mathematical cognition, arguing that there is evidence for the view that imagination is necessary both for the construction of figures, and for their presentation as abstractions subject to universal inference.
Finite Volume Scheme and Renormalized Solutions for a Noncoercive Elliptic Problem with
L
1 Data
Authors: Leclavier Sarah 
Published: November 2016

Abstract
The paper proves the convergence of the finite volume approximate solution
of a convection-diffusion equation with an

L
1

${L^{1}}$

right-hand side to the unique renormalized solution. The main difficulties are to handle the noncoercive character of the operator and the

L
1

${L^{1}}$

data. Mixing the techniques of renormalized solutions and the finite volume method allows one to derive estimates for the discrete solutions and in particular a discrete version of the decay of the truncated energy.
Indeed, as in the continuous case, the decay of the truncated energy is crucial to show that the limit of the approximate solution is the renormalized solution.