bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of proteins derived from dental pulp stem cells on human retinal pigment epithelial stem cells
Authors: Ghafourzadeh Masoumeh , Sichani Ziba Rezvani , Sichani Adel Rezvani , Rastegari Ali Asghar 
Published: September 2024

Abstract

Background: In recent years, stem cells have provided hopes for the treatment of many diseases. However, these cells are not easily replicable and passagable under cell culture conditions. The aim of this study is to investigate the impact of proteins derived from dental pulp stem cells on human retinal pigment epithelial cells.
Materials and Methods: Dental pulp stem cells were extracted following approved protocols at the Stem Cell Laboratory of Biotechnology Research Center under mycoplasma-free conditions. Subsequently, four concentrations of proteinase K were chosen: 100, 200, 300, and 400 µg/ml, and cells derived from dental pulp were incubated with these concentrations in adjacent medium for one hour. The metabolic activity of cells in different groups was assessed using the MTS assay at 3, 5, and 7 days, employing an ELISA reader for evaluation.
Results: The secreted substances extracted from human dental pulp stem cells have a proteinaceous nature. These secretions possess the ability to proliferate retinal pigment epithelial cells. However, by removing proteins from the neighboring environment conditioned by dental pulp stem cells, the proliferative effect is eliminated.
Conclusions: Treatment of the conditioned medium of dental pulp stem cells with proteinase K enzyme at a specific concentration, as determined by SDS-PAGE analysis, substantiates this claim.With the hope of utilizing secreted proteins in the treatment of more diseases.
Cascade-Synergistic Modulation of Intestinal Microbiota and Immune Microenvironment in Photothermal Orthotopic Colorectal Cancer Therapy
Authors: Chen Kaiwei , Liu Yazhou , Zhao Luyang , Shi Mengqian , Xing Ruirui , Yan Xuehai 
Published: September 2024

Abstract

Colorectal cancer (CRC) has become one of the major threats to public health. Surgical operation combined with chemotherapy intervention is currently the main clinical approach for treating malignant CRC. The progression of this malignancy is frequently associated with the disruptions to the intestinal microbiota and an immunosuppressive landscape within the tumor microenvironment, both of which contribute to an increased propensity for tumor recurrence and metastatic spread. However, traditional treatment strategies, as well as emerging tumor immunotherapies, often result in severe gastrointestinal adverse events (AEs) and a dysregulated immune microenvironment, which fail to make significant progress in the efficacy of CRC treatment. It is essential to explore a holistic approach that incorporates modulation of the microbial and immune microenvironments for the treatment of CRC. In this study, we provide an alternative transabdominal photothermal therapy (PTT) for CRC that utilizes a novelty designed photothermal functional assembled drug (MnBV@DPHA NPs) administered orally. We employ PTT to precisely ablate orthotopic tumors, and achieved a cascade synergistic modulation of the intestinal microbiota and immune microenvironment, thereby enhanced the therapeutic efficacy of CRC. The oral assembled drug effectively targeted the tumor, precisely thermo-ablated cancer lesion while sparing healthy tissue. The aforementioned treatment strategy significantly reduced the presence of pathogenic bacteria and increased the proportion of probiotics within the intestinal microenvironment, thereby restoring the intestinal microbiota’s homeostasis. Furthermore, it reshaped the immunosuppressive microenvironment of CRC, thereby maintaining an immune homeostasis. Further research has confirmed that the cascade synergistic between the intestinal microbiota and the immune microenvironment enhances the efficacy of PTT and inhibits the recurrence and metastasis of CRC.
Cosmetic Makeover of Periodontally Compromised Patients using Flexible Gingival Mask and Porcelain Laminate Veneers: Report of Two Cases
Authors: Gilra Shilpi 
Published: September 2024

Chronic periodontal disease can lead to advanced gingival tissue loss that can result in problems with esthetics, phonetics, food impaction and tooth sensitivity. One of the most difficult problem encountered in the management of periodontal disease is loss of interdental papillae which leaves unsightly “black triangles” between the teeth. The first step in the management of these problems is surgical correction of the defect. In cases where mucogingival surgery is unpredictable due to generalized recession and loss of large volume of gingival tissue, removable gingival prosthesis is an excellent alternative to correct the deformities remaining after the control of destructive periodontal disease specially in the maxillary anterior region. Gingival prostheses may be fixed or removable. It can be made from a range of materials like acrylic, silicones, porcelain- based materials and flexible resin denture material. This report describes two clinical situations in which combined periodontal/prosthodontic rehabilitation was done for patients with advanced periodontal disease. Accurately fitting flexible gingival prostheses and porcelain laminate veneers were fabricated to restore advanced gingival tissue loss in esthetic area following periodontal flap surgery. The flexible gingival masks not only help to achieve excellent cosmetic results but also reinstate the functional and psychological wellbeing of the patient.
Liquid Biopsy : A Narrative Review
Authors: HIMANSHU SINGH  , Dr. BHUPESH BHAYYAJI BAGULKAR  , DR. ARUNEE GARG  , DR.URVASHI TOMAR  , DR.ATUL BHAT  , DR.BHUVNESH AIREN  
Published: September 2024

Liquid biopsy has emerged as an important diagnostic method in the oncology discipline. Investigation of CTCs( circulating tumor cells), microRNAs, extracellular vesicles, and ctDNA(circulating tumor DNA, liquid biopsies allows understanding of the treatment feedback, tumor heterogeneity, individualized cancer treatment, and lowest residual disease recognition. Liquid biopsies show various advantages more favorable, less invasive, and more explanatory for directing personalized treatment recommendations. Liquid biopsies are being employed in clinical oncology, especially for those patients suffering from metastatic diseases.In this present review, the components and clinical applications of liquid biopsies are discussed so that we can understand about basics of liquid biopsy. In this review, literature or information on liquid biopsy is discussed. English language articles were scrutinize in plentiful directory or databases like Pubmed, Scopus, Web of Science, Science direct, Google scholar
Teeth, Tech, and AI: A Revolutionizing approach in Dentistry
Authors: Nahid Shazia 
Published: September 2024

Abstract
Introduction
Digital dentistry equipped with AI based integrated platforms enabled dentists to provide accurate diagnosis, prompt treatment, increased efficiency of evidence-based healthcare service provision.
Objectives

To explore the role of AI in dentistry with emphasis on public health aspects as oral health awareness, tobacco cessation counselling and tele Dentistry
to reconnoitre the role of AI in dental healthcare services and public health surveillance.

Methodology
Electronic search in various databases with the keywords AI, Digital dentistry, oral health awareness, surveillance, Communication, Dental imaging, were performed such as PubMed/MEDLINE (National Library of Medicine), Scopus (Elsevier), ScienceDirect databases (Elsevier), Web of Science (Clarivate Analytics), and the Cochrane Collaboration (Wiley). 30 full-text articles were selected and systematically analysed and the relevant data were extracted.
Result and Conclusion
AI-driven platform enhanced oral health awareness, services and facilitated automated evidence synthesis in the field of dentistry making it affordable, accessible and available to the public. Integration of AI and digital dentistry holds the promise of revolutionizing the way we approach oral health care.
Oral Myiasis: Case Report of a rare entity!
Authors: Purohit Dr Nikhil , Mishra Dr Manusmrati , Sharma Dr Ruchi , Jain Dr Prateek 
Published: September 2024

Background: Floods and severe rainfall are major reasons for the increase in infections like Dengue, malaria, and other parasitic infections. There may be an increase in worm infestations in the immediate post-flood period due to moist conditions. Oral myiasis is one of those rare parasitic infestations that may occur during such events and can be life-threatening to the patient due to its destructive nature. The chances become high if the patient has any underlying condition that can act as a reservoir or favourable host for such infestations. Immediate management of the disease increases the chances of a good prognosis and spread of the disease. Oral Myiasis is still a rare condition caused by larvae of flies called maggots. The following case report is an attempt to highlight the timely recognition of the disease which came to a private clinic with a routine complaint of toothache and its early management led to the complete recovery of the lesion and the patient.
Features of supply and approaches to the use of vitamin D in children in the post-COVID period
Authors: Bogomolova I. K. , Peregoedova V. N. 
Published: September 2024

Vitamin D is an important component of immune function, and deficiency in childhood is associated with an increased risk of acute respiratory viral infections. Purpose. To assess the dynamics of vitamin D supply in children after a new coronavirus infection with the use of cholecalciferol. Material and methods. We examined 55 children aged 0–17 years (10.0 [9.2; 10.6] years), 52.7 % boys with COVID-19, who received cholecalciferol in a dose of 1000 to 3000 IU for 30 days in depending on the concentration of 25(OH)D. After 30 days of taking vitamin D3, the levels of total vitamin D in the blood serum were re-determined. Results. The median 25(OH)D level in children with COVID-19 was 24.5 [23.9; 26.7] ng/ml. 34.5 % of children had vitamin D deficiency status (≤ 20 ng/ml), 32.7 % had insufficiency (21–30 ng/ml), a third of patients had normal vitamin D status (≥ 30 ng/ml). As a result of treatment with cholecalciferol, the number of children with hypovitaminosis D decreased by 2 times (35.4 %, p < 0.05), with an adequate level — doubled (65.5 %, p < 0.05). Depending on the level of 25(OH)D, no statistically significant differences were found in the severity of coronavirus infection (χ² = 0.872, p = 0.929). Conclusion. In 2/3 of cases, children infected with SARS-CoV-2 had hypovitaminosis D. Children under 6 years of age were more likely to have vitamin D insufficiency, while patients 7–17 years of age had a deficiency. The distribution of vitamin D levels did not differ depending on the severity of coronavirus infection. Doses of cholecalciferol taken for 30 days were sufficient to increase the concentration of vitamin D in the blood serum of children with deficiency but did not reach standard values in patients with vitamin D deficiency.
Significance of T-receptor and kappa-deletion recombina- tion excision rings as molecular markers in the assessment of newborns of different gestational ages
Authors: Volkova E. N. , Ippolitova L. I. 
Published: September 2024

Currently, there is a large number of studies indicating that preterm infants have reduced levels of T-receptor and kappa-deletion recombination excision rings (TREC and KREC) as indicators of impaired T- and/or B-cell immunity. Studies aimed at in-depth study of the causative factors that led to the decrease in the estimated indicators remain relevant. Purpose. Determination of the influence of somatometric parameters and prenatal factors on the level of TREC and KREC in newborns, as well as evaluation of the dynamics of these indicators from gestational age. Material and methods. The study included 203 neonates with gestational ages ranging from 22 to 41 weeks. TREC and KREC were isolated by PCR from dried blood spots on Guthrie cards. Blood sampling was performed as part of neonatal screening. The estimated parameters were analyzed according to gestational age, somatometric data and prenatal factors (mode of delivery, number of fetuses in pregnancy). Statistical analysis was performed using the StatTech v. 4.1.4 software. Results. It was found that the increase in gestational age by 1 week increases the KREC level by 44.610·105 (rxy = 0.271, p < 0.001), TREC level by 27.274·105 (rxy = 0.264, p = 0.002). Linear regression analysis showed weak direct relationships between TREC and KREC levels and anthropometric data. Children from multiple pregnancies had significantly higher TREC values than infants from singleton pregnancies (p < 0.001). Conclusion. The immune system of premature newborns is capable of producing adequate amounts of TREC and KREC. Between 22 and 28 weeks of age, the most intense increase in the assessed indicators occurs, after which their levels relatively stabilize. Since TREC and KREC levels tend to decrease in preterm newborns, a comprehensive evaluation of the dynamics of these indicators depending on significant prenatal and somatometric data is extremely important.
Phenotype and genotype features of isolated cystic kidney diseases with autosomal recessive type of inheritance in children
Authors: Andreeva E. F. , Savenkova N. D. 
Published: September 2024

The relevance of the problem of hereditary cystic kidney diseases (cystosis) is due to the wide variability of the renal phenotype and the genotype that determines the prognosis, the progression to renal failure as early as in childhood. Purpose. To present the results of the analysis of the correlation of genotype and phenotype, renal function in isolated cystic kidney diseases with an autosomal recessive type of inheritance in children. Material and methods. in 14 children (from 13 families) aged 9 months — 17 years, the features of the clinical phenotype of kidney cysts with autosomal recessive type of inheritance and gene mutation were evaluated according to the results of a molecular genetic study. Results. In 3 (23 %) of 13 families, the family history is burdened by kidney cysts. The characteristic of gene mutation in cystic kidney diseases with autosomal recessive type of inheritance in 13 patients is presented (in 2 tables). Of the 21 identified variants in the genes PKHD1, INVS, NPHP1, TMEM67, 15 (71 %) had known pathogenic significance, 6 (29 %) had previously undescribed variants in data-bases. Variants of the PKHD1 gene were identified in 11 children with the phenotype of polycystic kidney disease with autosomal recessive type of inheritance and liver fibrosis. In the study, variant C.107C>T (Thr36Met) is the most common among children with identified variants of the PKHD1 gene in autosomal recessive polycystic kidney disease in 5 out of 21 (23.8 %), c.664A>G (Ile222Val) in 1 (4.8 %), c.6992T>A (Ile2331Lys) in 1 (4.8 %), c.10444C>T (Arg3482Cys) at 1 (4.8 %). Infantile (n=1) and juvenile (n = 2) nephronophthysis were confirmed in 3 children with homozygous and compound heterozygous pathogenic variants in the INVS, NPHP1, TMEM67 genes. Conclusion. The features of the genotype and phenotype are presented in 14 children with autosomal recessive type of inheritance of isolated kidney cysts in autosomal recessive polycystic kidney disease (n = 11) and nephronophthysis (n = 3), of whom chronic kidney disease was established in 13 children aged 2–17 years: in 4 (30.8 %) C1, in 4 (30.8 %) C2, in 3 (23 %) C3, in 1 (7.7 %) C4, in 1 (7.7 %) C5.
A clinical case of multilocus imprinting disturbances: the first description in the Russian Federation
Authors: Panchenko E. G. , Vasyukova O. V. , Okorokov P. L. , Kopytina D. A. , Sigin V. O. , Strelnikov V. V. , Zaletaev D. V. 
Published: September 2024

Multilocus imprinting disturbances (MLID) is a molecular subtype of imprinting disorders (IDs), in which multiple methylation abnormalities of imprinted regions and genes are observed in combination with polymorphic clinical manifestations, including overlapping phenotypic features of individual imprinting disorders. The causes of MLID are mutations in genes encoding oocyte and zygotic factors of embryo development, which increases the risk of recurrent birth of children with imprinting disorders in mothers carrying such mutations. Due to the need to understand the exact risk for repeated childbirth, it is advisable to diagnose MLID in patients with an ambiguous phenotype and a negative result of studies on individual imprinting disorders, followed by a search for mutations in MLID-associated genes. The purpose of the work is to describe the clinical and epigenetic characteristics of a patient with MLID. A clinical case of a comorbid patient aged 12 years with an established molecular genetic diagnosis of MLID by the method of methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is presented. The features of the patient’s phenotype allow us to demonstrate the effect of hypomethylation of several differentially methylated regions of imprinted genes on the formation of a polymorphic phenotype, including overlapping signs of individual imprinting disorders, and to assess the difficulty of making an unambiguous clinical diagnosis for this patient. Pronounced clinical polymorphism, negative results of previously conducted molecular genetic studies on certain forms of imprinting disorders allow us to consider the MLID study as a first-line test for the diagnosis of methylation abnormalities in MLID and imprinting disorders.
Competencia cultural: las enfermeras que cuidan a los estudiantes en instituciones de salud universitarias
Authors: Orozco-Castillo Liliana , Pico-Merchán María Eugenia 
Published: September 2024

Introducción: los profesionales de enfermería se constituyen en pilar estratégico en las instituciones de salud universitarias, por tanto, éstos requieren tener competencias para brindar cuidados, desde una perspectiva cultural. Objetivo: describir cómo se expresa la competencia cultural en las enfermeras participantes y la percepción que tienen los estudiantes acerca de los cuidados recibidos. Método: se realizó una investigación cualitativa etnográfica con enfermeras de instituciones de salud universitarias y estudiantes regulares que recibieron cuidados de enfermería en la institución de salud. La recolección de información se hizo a través de observación participante y entrevista etnográfica. La información se analizó en el software ATLAS.ti versión 8.0. Resultados: participaron cinco enfermeras. Emergió el tema cultural: “conozco y disfruto el trabajo con los estudiantes”, “soy amable, los atiendo desde el ser y hago los procedimientos desde el saber”. Esto sustentado en dos patrones: Los estudiantes son diferentes y disfruto trabajar con ellos porque detecto situaciones y aprendo; y, soy amable, atiendo desde el ser y hago procedimientos desde el saber. Conclusiones: la competencia cultural en las enfermeras se expresa a partir del atributo de la amabilidad como elemento central en el cuidado a estudiantes universitarios y su desempeño laboral desde el saber.
Comparison of Amiodarone Loading Dosage in the Treatment of Postoperative Atrial Fibrillation: High Versus Standard Dose Treatment
Authors: Sarıçam Ersin , Öcal Arslan , Iscanlı Murat Doğan , Bozkurt Engin , Ilkay Erdogan , Cantekin Ömer Faruk 
Published: September 2024

Background:
Postoperative atrial fibrillation (POAF) is associated with poor outcomes,
including hemodynamic instability, stroke, myocardial infarction, and death. In hemodynamic stable
patients, the rhythm-control strategy is more advantageous than rate control. Current standard intravenous
amiodarone administration has limited success and a delayed effect; the acute success rate is
44% (8-12 h to several days).

Purpose:
The aim of this study was to evaluate the effectiveness of higher amiodarone loading dosage
to restore sinus rhythm in patients with POAF after noncardiac surgery.

Methods:
This is a prospective, randomized, controlled single-center study. The study included 39
patients with POAF, divided into group I (n=27) (intravenous 600 mg amiodarone loading dosage
over 2 h and infusion of 50 mg/h over a 24-h period) and group II (n=12) (standard protocol; 300 mg
of bolus intravenously in 30 min and infusion of 50 mg/h over a 24-h period). The primary endpoint
of the study was a restoration of sinus rhythm at the 24th hour.

Results:
Baseline clinical, laboratory and echocardiographic characteristics of both groups were similar.
The patients with higher loading amiodarone dosage had earlier restoration of sinus rhythm (2.38
± 1.41 vs 8.66 ± 2.87 h, respectively; p=0.015). There was no significant difference in achieving sinus
rhythm at the 24th hour between both groups.

Conclusion:
Higher loading amiodarone dosage increased early conversions to sinus rhythm compared
with standard amiodarone protocol in patients with POAF.
Mapping the Landscape of Global Anticancer Organometallics Research: A Systematic Review
Authors: Ur Rehman Tanzeel , Abdulaziz Alnaam Yaser , Zahid Misbah , Algethami Jari S. 
Published: September 2024

Aim:
Due to interdisciplinary research, many innovative concepts have been merged that
seemed to be impractical. Recently, medicinal organometallic chemistry has made remarkable progress,
but the latency of these compounds has not been fully exploited. This systematic review has
examined the published literature on anticancer organometallic chemistry across countries, science
fields, and organizations involved in organometallics research for cancer.

Method:
The study data related to anticancer organometallics were searched from Scopus between
2085 and 2022. Biblioshiny and VOS Viewers were used to analyze and visualize patterns in scientific
literature derived from Scopus.

Results:
Publications on organometallic compounds have been found to contribute to, on average,
1.02% per year, accounting for 94.3% of the total scholarly work published in the last two decades
since 2003. However, research productivity has been found to be steadily improved, with 81.5% of
all publications produced between 2011 and 2022. The countries possessing the highest published
work have been found to be China, the UK, and Germany. The leading institutions, the University
of Warwick, United Kingdom, and the University of Auckland, New Zealand, have topped the list
of organizations with the most publications. Although the use of medicinal organometallics for
cancer has become widespread over the last two decennaries, there has been a notable influx of
groundbreaking scientific publications in recent years.

Conclusion:
The findings of this study may enable researchers to envision potential future scenarios
for scientific collaborations involving the utilization of organometallics in the treatment of cancer.
This study may provide aspiring and current researchers with the necessary tools and
knowledge to effectively pursue their research endeavors for scientific collaborations investigating
the use of anticancer organometallics in the medicinal field. The areas, such as ruthenium with
reactive oxygen species and angiogenesis, represent opportunities for future investigation and innovation.