bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon
Authors: Liu Derui , Zehfroosh Nina , Hancock Brandon L. , Hines Kevin , Fang Wenjuan , Kilfoil Maria , Learned-Miller Erik , Sanguinet Karen A. , Goldner Lori S. , Baskin Tobias I. 
Published: November 2017

AbstractThe mechanism of cellulose synthesis has been studied by characterizing the motility of cellulose synthase complexes tagged with a fluorescent protein; however, this approach has been used exclusively on the hypocotyl of Arabidopsis thaliana. Here we characterize cellulose synthase motility in the model grass, Brachypodium distachyon. We generated lines in which mEGFP is fused N-terminal to BdCESA3 or BdCESA6 and which grew indistinguishably from the wild type (Bd21-3) and had dense fluorescent puncta at or near the plasma membrane. Measured with a particle tracking algorithm, the average speed of GFP-BdCESA3 particles in the mesocotyl was 164 ± 78 nm min−1 (error gives standard deviation [SD], n = 1451 particles). Mean speed in the root appeared similar. For comparison, average speed in the A. thaliana hypocotyl expressing GFP-AtCESA6 was 184 ± 86 nm min−1 (n = 2755). For B. distachyon, we quantified root diameter and elongation rate in response to inhibitors of cellulose (dichlorobenylnitrile; DCB), microtubules (oryzalin), or actin (latrunculin B). Neither oryzalin nor latrunculin affected the speed of CESA complexes; whereas, DCB reduced average speed by about 50% in B. distachyon and by about 35% in A. thaliana. Evidently, between these species, CESA motility is well conserved.
Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology
Authors: Abe Satoshi , Kobayashi Kaoru , Oji Asami , Sakuma Tetsushi , Kazuki Kanako , Takehara Shoko , Nakamura Kazuomi , Okada Azusa , Tsukazaki Yasuko , Senda Naoto , Honma Kazuhisa , Yamamoto Takashi , Ikawa Masahito , Chiba Kan , Oshimura Mitsuo , Kazuki Yasuhiro 
Published: November 2017

AbstractCytochrome P450, family 3, subfamily A (CYP3A) enzymes metabolize approximately 50% of commercially available drugs. Recently, we developed fully humanized transchromosomic (Tc) CYP3A mice with the CYP3A cluster including CYP3A4, CYP3A5, CYP3A7, and CYP3A43. Our humanized CYP3A mice have the CYP3A5*3 (g.6986G) allele, resulting in the almost absence of CYP3A5 protein expression in the liver and intestine. To produce model mice for predicting CYP3A5′s contribution to pharmacokinetics, we performed a single-nucleotide polymorphism (SNP) modification of CYP3A5 (g.6986G to A, *3 to *1) on the CYP3A cluster using genome editing in both mouse ES cells and fertilized eggs, and produced humanized CYP3A5*1 mice recapitulating the CYP3A5*1 carrier phenotype in humans. The humanized CYP3A mouse with CYP3A5*1 is the first Tc mouse for predicting the SNP effect on pharmacokinetics in humans. The combination of Tc technology and genome editing enables the production of useful humanized models that reflect humans with different SNPs.
Substrate Microarchitecture Shapes the Paracrine Crosstalk of Stem Cells with Endothelial Cells and Osteoblasts
Authors: Martín-Saavedra Francisco , Crespo Lara , Escudero-Duch Clara , Saldaña Laura , Gómez-Barrena Enrique , Vilaboa Nuria 
Published: November 2017

AbstractWe examined the hypothesis that substrate microarchitecture regulates the crosstalk between human mesenchymal stem cells (hMSC) and cell types involved in bone regeneration. Compared with polyester flat substrates having uniformly distributed homogenous pores (2D), three-dimensional polystyrene substrates with randomly oriented and interconnected pores of heterogeneous size (3D) stimulated the stromal secretion of IGF-1 while lessened the production of VEGFR-1, MCP-1 and IL-6. The medium conditioned by hMSC cultured in 3D substrates stimulated tube formation by human endothelial cells (hEC) to a higher extent than medium from 2D cultures. 3D co-cultures of hMSC and hEC contained higher secreted levels of IGF-1, EGF and FGF-2 than 2D co-cultures, resulting in increased hEC proliferation and migration. Substrate microarchitecture influenced the secretion of factors related to bone remodeling as the ratio RANKL to OPG, and the levels of M-CSF and IL-6 were higher in 3D co-cultures of hMSC and human osteoblasts (hOB) than in 2D co-cultures. Cytokine microenvironment in 3D co-cultures stimulated osteoblast matrix reorganization while demoted the late steps of osteoblastic maturation. Altogether, data in this study may unveil a new role of scaffold microarchitecture during bone regeneration, as modulator of the paracrine relationships that hMSC establish with hEC and hOB.
Productivity benefits of warming at regional scale could be offset by detrimental impacts on site level hydrology
Authors: Zeng Qing , Zhang Yamian , Wen Li , Li Zhaxijie , Duo Hairui , Lei Guangchun 
Published: November 2017

AbstractClimate change affects the distribution and persistence of wildlife. Broad scale studies have demonstrated that climate change shifts the geographic ranges and phenology of species. These findings are influential for making high level strategies but not practical enough to guide site specific management. In this study, we explored the environment factors affecting the population of Bar-headed Goose in the key breeding site of Qinghai using generalized additive mixed model (GAMM). Our results showed that 1) there were significant increasing trends in climate variables and river flows to the Qinghai Lake; 2) NDVI in the sites decreased significantly despite the regional positive trend induced by the warmer and wetter climate; 3) NDVI at site scale was negatively correlated to lake water level; and 4) the abundance of Bar-headed Goose decreased significantly at all sites. While the abundance was positively related to NDVI at breeding sites, the GAMM revealed an opposite relationship at foraging areas. Our findings demonstrated the multi-facet effects of climate change on population dynamics; and the effect at global/regional scale could be complicated by site level factors.
Polymer Nanodiscs: Discoidal Amphiphilic Block Copolymer Membranes as a New Platform for Membrane Proteins
Authors: Fiori Mariana C. , Jiang Yunjiang , Zheng Wan , Anzaldua Miguel , Borgnia Mario J. , Altenberg Guillermo A. , Liang Hongjun 
Published: November 2017

AbstractLipid nanodiscs are playing increasingly important roles in studies of the structure and function of membrane proteins. Development of lipid nanodiscs as a membrane-protein-supporting platform, or a drug targeting and delivery vehicle in general, is undermined by the fluidic and labile nature of lipid bilayers. Here, we report the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer membrane patches encased within membrane scaffold proteins, as a novel two-dimensional nanomembrane that maintains the advantages of lipid nanodiscs while addressing their weaknesses. Using MsbA, a bacterial ATP-binding cassette transporter as a membrane protein prototype, we show that the protein can be reconstituted into the polymer nanodiscs in an active state. As with lipid nanodiscs, reconstitution of detergent-solubilized MsbA into the polymer nanodiscs significantly enhances its activity. In contrast to lipid nanodiscs that undergo time- and temperature-dependent structural changes, the polymer nanodiscs experience negligible structural evolution under similar environmental stresses, revealing a critically important property for the development of nanodisc-based characterization methodologies or biotechnologies. We expect that the higher mechanical and chemical stability of block copolymer membranes and their chemical versatility for adaptation will open new opportunities for applications built upon diverse membrane protein functions, or involved with drug targeting and delivery.
Benchmarking integrated linear-optical architectures for quantum information processing
Authors: Flamini Fulvio , Spagnolo Nicolò , Viggianiello Niko , Crespi Andrea , Osellame Roberto , Sciarrino Fabio 
Published: November 2017

AbstractPhotonic platforms represent a promising technology for the realization of several quantum communication protocols and for experiments of quantum simulation. Moreover, large-scale integrated interferometers have recently gained a relevant role in quantum computing, specifically with Boson Sampling devices and the race for quantum supremacy. Indeed, various linear optical schemes have been proposed for the implementation of unitary transformations, each one suitable for a specific task. Notwithstanding, so far a comprehensive analysis of the state of the art under broader and realistic conditions is still lacking. In the present work we fill this gap, providing in a unified framework a quantitative comparison of the three main photonic architectures, namely the ones with triangular and square designs and the so-called fast transformations. All layouts have been analyzed in presence of losses and imperfect control over the internal reflectivities and phases, showing that the square design outperforms the triangular scheme in most operational conditions. Our results represent a further step ahead towards the implementation of quantum information protocols on large-scale integrated photonic devices.
Enzymatic degradation of organophosphorus insecticides decreases toxicity in planarians and enhances survival
Authors: Poirier Laetitia , Brun Lucile , Jacquet Pauline , Lepolard Catherine , Armstrong Nicholas , Torre Cédric , Daudé David , Ghigo Eric , Chabrière Eric 
Published: November 2017

AbstractOrganophosphorus insecticides (OPs) are toxic compounds used for agricultural purposes and responsible for severe types of contamination worldwide. OPs may also induce chronic deleterious effects and developmental disruption. Finding remediation strategies is a major concern to diminish their impact on environment and human health. Enzymes have emerged as a promising eco-friendly route for decontaminating OPs. The enzyme SsoPox from the archaea Sulfolobus solfataricus has been particularly studied, considering both its tremendous stability and phosphotriesterase activity. However, the toxicity of the degradation products generated through enzyme hydrolysis has been poorly investigated. To address both neurotoxicity and developmental perturbation, freshwater planarians from Platyhelminthes were considered to evaluate the impact of OP and degradation product exposure. Planarians have a large proportion of stem cells that give them an unconventional capacity for regeneration. OPs were found to be highly toxic to planarians and enzyme decontamination drastically enhanced survival rate. Although not completely innocuous, the degradation products were found to be less toxic than insecticides and reduced poisoning effects by increasing NOEC values by up to eight-fold. SsoPox also limited detrimental consequences on planarian mobility and enabled them to recover a non-exposed type regeneration process suggesting that enzymatic decontamination is a promising alternative to bioremediation.
Pinning and hysteresis in the field dependent diameter evolution of skyrmions in Pt/Co/Ir superlattice stacks
Authors: Zeissler K. , Mruczkiewicz M. , Finizio S. , Raabe J. , Shepley P. M. , Sadovnikov A. V. , Nikitov S. A. , Fallon K. , McFadzean S. , McVitie S. , Moore T. A. , Burnell G. , Marrows C. H. 
Published: November 2017

AbstractWe have imaged Néel skyrmion bubbles in perpendicularly magnetised polycrystalline multilayers patterned into 1 µm diameter dots, using scanning transmission x-ray microscopy. The skyrmion bubbles can be nucleated by the application of an external magnetic field and are stable at zero field with a diameter of 260 nm. Applying an out of plane field that opposes the magnetisation of the skyrmion bubble core moment applies pressure to the bubble and gradually compresses it to a diameter of approximately 100 nm. On removing the field the skyrmion bubble returns to its original diameter via a hysteretic pathway where most of the expansion occurs in a single abrupt step. This contradicts analytical models of homogeneous materials in which the skyrmion compression and expansion are reversible. Micromagnetic simulations incorporating disorder can explain this behaviour using an effective thickness modulation between 10 nm grains.
Effect of vitamin D supplementation on inflammation and nuclear factor kappa-B activity in overweight/obese adults: a randomized placebo-controlled trial
Authors: Mousa Aya , Naderpoor Negar , Johnson Josphin , Sourris Karly , de Courten Maximilian P. J. , Wilson Kirsty , Scragg Robert , Plebanski Magdalena , de Courten Barbora 
Published: November 2017

AbstractIn-vitro studies suggest that vitamin D reduces inflammation by inhibiting nuclear factor kappa-B (NFκB) activity. Yet, no trials have examined the effects of vitamin D supplementation on NFκB activity in-vivo in humans. We conducted a double-blind randomized trial (RCT) examining effects of vitamin D supplementation on inflammatory markers and NFκB activity in peripheral blood mononuclear cells (PBMCs). Sixty-five overweight/obese, vitamin D-deficient (25-hydroxyvitamin D [25(OH)D] ≤ 50 nmol/L) adults were randomized to a single 100,000 IU bolus followed by 4,000 IU daily cholecalciferol or matching placebo for 16 weeks. We measured BMI, % body fat, serum 25(OH)D, high-sensitivity C-reactive protein (hsCRP), tumour necrosis factor (TNF), monocyte chemoattractant protein-1 (MCP-1), interferon-gamma (IFN-γ), several interleukins, and NFκB activity in PBMCs. Fifty-four participants completed the study. Serum 25(OH)D concentrations increased with vitamin D supplementation compared to placebo (p < 0.001). Vitamin D and placebo groups did not differ in any inflammatory markers or NFκB activity (all p > 0.05). Results remained non-significant after adjustment for age, sex, and % body fat, and after further adjustment for sun exposure, physical activity, and dietary vitamin D intake. Although in-vitro studies report anti-inflammatory effects of vitamin D, our RCT data show no effect of vitamin D supplementation on inflammatory markers or NFκB activity in-vivo in humans.
Crickets alter wind-elicited escape strategies depending on acoustic context
Authors: Fukutomi Matasaburo , Ogawa Hiroto 
Published: November 2017

AbstractAcoustic signals trigger various behaviours in insects such as courtship or escape from predators. However, it remains unknown whether insects utilize acoustic signals to recognize environmental contexts. The cricket is a prominent model insect for neuroethological studies on acoustic behaviour because female crickets exhibit positive phonotaxis in response to male calling songs, and flying crickets display avoidance behaviour for high-frequency sounds such as echolocation call of bats. The carrier frequency of these sounds is a major factor in determining whether they initiate these acoustic behaviours. Here, we examined the impacts of different frequencies of tone sounds on cercal-mediated escape behaviour, using a 5-kHz tone corresponding to the calling song and a 15-kHz tone serving as a trigger of avoidance behaviours. Neither frequency elicited a response in the standing cricket by itself, but they had different impacts on walking responses to airflow stimuli. While the 15-kHz tone reduced response probability, extended moving distance, and enhanced turn-angle variability, the 5-kHz tone had no effect. Although both frequencies of tones facilitated walking backward, the 15-kHz tone had a larger effect than the 5-kHz tone. These frequency dependencies of behavioural modulation suggest that crickets can recognize acoustic contexts and alter their escape strategy accordingly.
Effects of Cement Paste Enhanced with Iron-Based Magnetic Particles on an Embedded Small Resonator Antenna
Authors: Sum Yee Loon , Rheinheimer Vanessa , Soong Boon Hee , Monteiro Paulo J. M. 
Published: November 2017

AbstractSmall resonator antennas, such as metaresonator antennas, have narrow bandwidths, which limits their effective range of frequencies. When they are used as embedded antennas in building materials, their performance is affected more than other types of antennas, as typical building materials have a shielding effectiveness (SE) of 80 dB to 100 dB. Adding magnetic and/or metallic particles to cement mixes changes the properties of the concrete, which can improve the performance of antennas. Specifically, enhancing a cement paste with iron-based magnetic particles improves the bandwidth and S11 of embedded antennas. This report investigates the impact of two different iron-based magnetic particle sizes (micro- and nanosized particles) to determine the effects that they have on the S11 and S21 characteristics of the metaresonator antenna array embedded in enhanced cement pastes. Results show that compared to cement paste only sample, cement paste with micro-sized iron-based magnetic particles had the greatest improvement of performance of a metaresonator antenna array in terms of a small shift in the resonance frequency and an increase of bandwidth. Particularly for a cement paste enhanced with micro-sized iron (III) oxide particles, the S21 curve was improved over the cement paste only sample by as much as 10 dB.
Birth of clones of the world’s first cloned dog
Authors: Kim Min Jung , Oh Hyun Ju , Kim Geon A , Setyawan Erif Maha Nugraha , Choi Yoo Bin , Lee Seok Hee , Petersen-Jones Simon M. , Ko CheMyong J. , Lee Byeong Chun 
Published: November 2017

AbstractAnimal cloning has gained popularity as a method to produce genetically identical animals or superior animals for research or industrial uses. However, the long-standing question of whether a cloned animal undergoes an accelerated aging process is yet to be answered. As a step towards answering this question, we compared longevity and health of Snuppy, the world’s first cloned dog, and its somatic cell donor, Tai, a male Afghan hound. Briefly, both Snuppy and Tai were generally healthy until both developed cancer to which they succumbed at the ages of 10 and 12 years, respectively. The longevity of both the donor and the cloned dog was close to the median lifespan of Afghan hounds which is reported to be 11.9 years. Here, we report creation of 4 clones using adipose-derived mesenchymal stem cells from Snuppy as donor cells. Clinical and molecular follow-up of these reclones over their lives will provide us with a unique opportunity to study the health and longevity of cloned animals compared with their cell donors.
Lung Clearance Index and Quantitative Computed Tomography of Post-Infectious Bronchiolitis Obliterans in Infants
Authors: Kim Yoon Hee , Shin Hyun Joo , Sol In Suk , Kim Soo Yeon , Kim Jong Deok , Yoon Haesung , Kim Kyung Won , Kim Myung-Joon , Lee Mi-Jung , Sohn Myung Hyun 
Published: November 2017

AbstractPost-infectious bronchiolitis obliterans (BO) could be diagnosed via spirometry and chest computed tomography (CT); however, these tests are limited in infants. We aimed to evaluate the utility of lung clearance index (LCI) and air-trapping lung volume from chest CT in infants. This prospective study included 20 infants (mean age, 10.9 ± 6.3 months) diagnosed with post-infectious BO between 2009 and 2016. All subjects underwent multiple breath washout tests. For quantitative analysis of chest CT, the mean lung area attenuation value was used as an individual cutoff to determine the air-trapping lung volume. The mean cutoff lung attenuation value was −659 Hounsfield units, the mean total lung volume was 265 ml, and the mean air-trapping lung volume percentage was 22.9%. Functional residual capacity correlated with total lung volume and normal attenuation lung volume (p < 0.02). LCI (p < 0.02) and moment ratio (MR) 1 (p < 0.05) correlated with the air-trapping lung volume percentage. The concordance indices of LCI (0.659, p = 0.025) and MR1 (0.642, p = 0.046) were significantly correlated with the air-trapping lung volume percentage from CT. LCI and quantitative air-trapping lung volume from chest CT are feasible, complimentary tools for assessing infants with post-infectious BO.
Short-term effects of non-grazing on plants, soil biota and aboveground-belowground links in Atlantic mountain grasslands
Authors: Epelde Lur , Lanzén Anders , Mijangos Iker , Sarrionandia Estibaliz , Anza Mikel , Garbisu Carlos 
Published: November 2017

AbstractMountain grasslands in the Iberian Peninsula are the result of extensive grazing. However, a progressive abandonment of grazing activity is currently observed in the study region. The objective of this work was to evaluate the short-term (2 years) effects of non-grazing on the diversity and composition of plants, soil microorganisms (prokaryotes, fungi, arbuscular mycorrhiza), mesofauna, macrofauna and aboveground-belowground links, through the study of 16 grazed vs. non-grazed areas in Atlantic grasslands located in the Basque Country (Spain). Sites were divided between 4 habitat types with different elevation, pasture productivity, vegetation type and parent material. Herbivores appeared to influence plant community composition, contributing to increase aboveground diversity, while having unequal effects on belowground communities depending on the organisms analysed. This may be explained by the different habitat and trophic level of each soil organism, which may be more or less affected by the predominating negative effects of grazing, such as soil compaction, and only partially compensated by other positive effects. Finally, habitat type appeared to be the strongest influence on both above- and belowground communities, also influencing the effect of the absence of grazing.
Chromatin Protamination and Catsper Expression in Spermatozoa Predict Clinical Outcomes after Assisted Reproduction Programs
Authors: Marchiani S. , Tamburrino L. , Benini F. , Fanfani L. , Dolce R. , Rastrelli G. , Maggi M. , Pellegrini S. , Baldi E. 
Published: November 2017

AbstractIdentification of parameters predicting assisted reproductive technologies (ARTs) success is a major goal of research in reproduction. Quality of gametes is essential to achieve good quality embryos and increase the success of ARTs. We evaluated two sperm parameters, chromatin maturity and expression of the sperm specific calcium channel CATSPER, in relation to ART outcomes in 206 couples undergoing ARTs. Chromatin maturity was evaluated by Chromomycin A3 (CMA3) for protamination and Aniline Blue (AB) for histone persistence and CATSPER expression by a flow cytometric method. CMA3 positivity and CATSPER expression significantly predicted the attainment of good quality embryos with an OR of 6.6 and 14.3 respectively, whereas AB staining was correlated with fertilization rate. In the subgroup of couples with women ≤35 years, CATSPER also predicted achievement of clinical pregnancy (OR = 4.4). Including CMA3, CATSPER and other parameters affecting ART outcomes (female age, female factor and number of MII oocytes), a model that resulted able to predict good embryo quality with high accuracy was developed. CMA3 staining and CATSPER expression may be considered two applicable tools to predict ART success and useful for couple counseling. This is the first study demonstrating a role of CATSPER expression in embryo development after ARTs programs.