bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary establishment of genetic transformation system for embryogenic callus of Acer truncatum ‘Lihong’
Authors: Yang Yipeng , Chan Yuan , Wang Yongge , Guo Hao , Song Lina , Zhang Huali , Sun Liping , Cong Richen , Zhang Hua 
Published: September 2024

IntroductionAcer truncatum Bunge, belonging to the Acer genus in the Aceraceae family, is a commonly planted afforestation species across China, Japan, Korea, Europe, and North America. Renowned for its vibrant fall colors, it holds significant ecological and ornamental value.MethodsIn this study, Acer truncatum ' Lihong ' was used as the research object. Starting from the callus induction of explants, the embryogenic callus of Acer truncatum 'Lihong' was obtained by systematically optimizing the medium and culture conditions. Then, the candidate gene AtrGST894 screened by transcriptome sequencing was transformed into embryogenic callus by Agrobacterium-mediated transformation. The genetic transformation system of Acer truncatum 'Lihong' embryogenic callus was initially established by continuously adjusting the conditions of Agrobacterium tumefaciens infection receptor materials, thus laying a material foundation for the study of the molecular regulation mechanism of Acer truncatum 'Lihong' leaf color, and also preparing for the later molecular improvement breeding of Acer truncatum. Therefore, this study has important theoretical and practical significance.ResultsThe results showed that the best medium for callus induction of Acer truncatum was 1/2MS+2 mg/L 2,4-D+0.3 mg/L 6-BA+0.5 mg/L NAA; The embryogenic callus induction medium of Acer truncatum was 1/2MS+3.0mg/L 6-BA+2.0mg/L TDZ+0.5mg/L IBA+0.1mg/L GA3; The proliferation medium of embryogenic callus of Acer truncatum was WPM+1.0mg/L TDZ+0.5mg/L IBA+0.1mg/L GA3+3mg/L 6-BA+1.0mg/L KT; The infection experiment of Agrobacterium tumefaciens on the embryogenic callus of Acer truncatum showed that the best antibacterial medium was WPM+30g/L sucrose+8g/L agar+0.5g/L acid-hydrolyzed casein+0.2mg/L KT+1.0 mg/L TDZ+0.5 mg/L IBA+0.1 mg/L GA3+200mmol/L carboxybenzyl+200mg/L cephalosporin, and then WPM+30g/L sucrose+8g/L agar+0.5g/L acid-hydrolyzed casein+0.2mg/L KT+1.0 mg/L TDZ+0.5 mg/L IBA+0.1 mg/L GA3+300mmol/L carboxybenzyl+200mg/L cephalosporin+25mg/L hygromycin. Screening medium screening, The obtained embryogenic callus browning rate, pollution rate and mortality rate were the lowest, and maintained vigorous growth.DiscussionThe embryogenic callus was used as the infection material to verify that we successfully transferred the target gene into the embryogenic callus, which means that the genetic transformation system of Acer truncatum embryogenic callus was partially completed, and the infection process could be effectively inhibited. Although there was partial browning, it could continue to proliferate. Therefore, in future experiments, the focus is still to continue to verify the optimal conditions for optimizing the genetic transformation of Acer truncatum embryogenic callus and to solve the problems of difficulty in embryonic callus germination.
Methane-derived microbial biostimulant reduces greenhouse gas emissions and improves rice yield
Authors: Kumar Sarma Rajeev , David Einstein Mariya , Pavithra Gangigere Jagadish , Kumar Gopalakrishnan Sajith , Lesharadevi Kuppan , Akshaya Selvaraj , Basavaraddi Chavadi , Navyashree Gopal , Arpitha Panakanahalli Shivaramu , Sreedevi Padmanabhan , Zainuddin Khan , Firdous Saiyyeda , Babu Bondalakunta Ravindra , Prashanth Muralidhar Udagatti , Ravikumar Ganesan , Basavaraj Palabhanvi , Chavana Sandeep Kumar , Kumar Vinod Munisanjeeviah Lakshmi Devi , Parthasarathi Theivasigamani , Subbian Ezhilkani 
Published: September 2024

IntroductionMore than half of the world’s population consumes rice as their primary food. The majority of rice production is concentrated in Asia, with the top 10 rice-growing countries accounting for 84% of the world’s total rice cultivation. However, rice production is also strongly linked to environmental changes. Among all the global sources of greenhouse gas (GHG) emissions, paddy cultivation stands out as a significant contributor to global methane (CH4) and nitrous oxide (N2O) emissions. This contribution is expected to increase further with the projected increase of 28% in global rice output by 2050. Hence, modifications to rice management practices are necessary both to increase yield and mitigate GHG emissions.MethodsWe investigated the effect of seedling treatment, soil application, and foliar application of a methane-derived microbial biostimulant on grain yield and GHG emissions from rice fields over three seasons under 100% fertilizer conditions. Further, microbial biostimulant was also tested under 75% nitrogen (N) levels to demonstrate its effect on grain yield. To understand the mechanism of action of microbial biostimulant on crop physiology and yield, a series of physiological, transcript, and metabolite analyses were also performed.ResultsOur three-season open-field studies demonstrated a significant enhancement of grain yield, up to 39%, with a simultaneous reduction in CH4 (31%–60%) and N2O (34%–50%) emissions with the use of methane-derived microbial biostimulant. Under 75% N levels, a 34% increase in grain yield was observed with microbial biostimulant application. Based on the physiological, transcript, and metabolite analyses data, we were further able to outline the potential mechanisms for the diverse synergistic effects of methane-derived microbial biostimulant on paddy, including indole-3-acetic acid production, modulation of photosynthesis, tillering, and panicle development, ultimately translating to superior yield.ConclusionThe reduction in GHG emission and enhanced yield observed under both recommended and reduced N conditions demonstrated that the methane-derived biostimulant can play a unique and necessary role in the paddy ecosystem. The consistent improvements seen across different field trials established that the methane-derived microbial biostimulant could be a scalable solution to intensify rice productivity with a lower GHG footprint, thus creating a win–win–win solution for farmers, customers, and the environment.
Low self-control, perceived social support and internet gaming addiction: findings from an ethnicity minority region in China
Authors: Meng Cui , Quancai Li , Kunjie Cui , Yanyu Xin , Wencai Lama , Yiwei Xia 
Published: September 2024

BackgroundAdolescent Internet gaming addiction (IGA) is an pincreasing global concern. Drawing on self-control theory, social support theory, and problem behavior theory, this study aimed to examine the relationships between low self-control, perceived social support, and IGA among Chinese Yi and Non-Yi adolescents, with a focus on the moderating role of perceived social support and ethnic differences.MethodsA cross-sectional study was conducted among 1,997 adolescents (53.78% female, mean age 14.70 years) in Liangshan Yi Autonomous Prefecture, Sichuan Province, China, using a multi-stage cluster random sampling method. Participants completed questionnaires assessing IGA (Internet Gaming Disorder Scale-Short Form), low self-control (Low Self-Control Scale), and perceived social support (Multidimensional Scale of Perceived Social Support). Data were analyzed using descriptive statistics, t-tests, OLS regression, and the seemingly unrelated estimator (SUE).ResultsLow self-control was positively associated with IGA (β = 0.35, p &lt; 0.001), while perceived social support was negatively associated with IGA (β = -0.27, p &lt; 0.001). Perceived social support, particularly from family (β = -0.43, p &lt; 0.05) and significant others (β = -0.49, p &lt; 0.01), moderated the relationship between low self-control and IGA. These associations were more salient among Yi adolescents compared to non-Yi adolescents.ConclusionsThis study highlights the protective role of perceived social support, especially from family and significant others, in buffering the risk of low self-control on IGA. The findings extend self-control theory and social support theory, and provide empirical support for problem behavior theory in a cross-cultural context. The results underscore the importance of considering cultural contexts in understanding IGA and developing targeted interventions for ethnic minority adolescents.
Effect of ginseng stem leaf extract on the production performance, meat quality, antioxidant status, immune function, and lipid metabolism of broilers
Authors: Zhang Peng , Zhang Haoyue , Ma Chuanjie , Lv Qiufeng , Yu Haiyang , Zhang Qiang 
Published: September 2024

IntroductionThe present study explores the effect of ginseng stem leaf (GSL) extract on the production performance, meat quality, antioxidant status, immune function, and lipid metabolism of white feathered broilers.MethodsThere were 6 replicates in each group, with 10 broilers in each replicate. In the 42 day trial, 300 AA broilers were randomly divided into five groups: control group (CON), 1.25% GSL extract group (GSL-L), 2.5% GSL group (GSL-M), 5% GSL group (GSL-H), and 45 mg/kg chlortetracycline group (CTC).ResultsThe results showed that different doses of GSL extract could improve the body weight, feed to gain ratio (F/G), average daily feed intake (ADFI), average daily gain (ADG), and meat quality of broilers. Compared with the control group, the addition of different doses of GSL improved the antioxidant and immune abilities of broilers to varying degrees, and the effect of GSL extract was significant in the GSL-H group (p &lt; 0.05). In addition, medium and high doses of GSL extract significantly reduced the blood triglyceride (TG) and total cholesterol (TC) contents of broilers (p &lt; 0.05).DiscussionAdding GSL extract to the feed has a positive impact on the body weight, meat quality, antioxidant capacity, immunity, and blood lipids of broilers.
THE BASIC PRINCIPLES OF BUILDING MODELS OF PROMISING HYBRID GROUND-SPACE COMMUNICATION SYSTEMS
Authors: Пантелеймонов И.Н. 
Published: September 2024

Представлено описание моделей и уровней взаимодействия технологий, систем и элементов с целью формирования системного подхода, необходимого для проведения анализа и синтеза перспективных гибридных наземно-космических систем связи.
A description of models and levels of interaction of technologies, systems and elements is presented in order to form a system approach necessary for the analysis and synthesis of advanced hybrid ground-space communication systems.
NALYSIS OF A FINE-CRYSTALLINE CORUNDUMRECRYSTALLIZATION IN SUPERCRITICALWATER MEDIUM USING THE LOGNORMALPARTICLE SIZE DISTRIBUTIONFUNCTION
Authors: Ивакин Ю.Д. , Данчевская М.Н. 
Published: September 2024

Рассмотрен механизм синтеза мелкокристаллического корунда из гидроксидаалюминия в условиях индуцированного зародышеобразования в среде сверхкритического водного флюида при температуре 400 °С, начальном давлении 26,4 МПаи последующей продолжительной выдержке в среде синтеза. Распределение поразмерам кристаллов строилось на основе анализа электронно-микроскопических фотографий. Обосновано использование логнормальной функции для описания распределения по размерам кристаллов синтезированного порошка корунда. Проанализирована возможность использования логнормальной функциираспределения по размерам кристаллов синтезированного порошка корунда изависимости от времени ее параметров для выявления маршрутов формирования кристаллов продукта. Установлено, что размерный спектр микрокристалловимеет четыре компонента, появление которых связано с различными маршрутами их формирования. Число компонентов распределения сохраняется в ходепоследующей изотермической выдержки, но их средние характеристики имеютразличную зависимость от времени. Изменение долей компонентов при рекристаллизации, приводящее в целом к уменьшению среднего размера кристаллов, объяснено отличиями в подвижности структуры кристаллов разных компонентов. Сделан вывод о различии состояния корунда во вновь образованных кристаллах инаращенного на частицы индуцирующей добавки.
A mechanism of the fine-crystalline corundum synthesis from aluminum hydroxide inthe conditions of induced nuclеation in the supercritical water fluid at 400°C, aninitial pressure of 26.4 MPa, and subsequent exposure in a synthesis medium is considered.The size distribution of the crystals was based on the analysis of electron microscopicimages. The use of the lognormal function to describe the crystal size distribution ofthe synthesized corundum particles is substantiated. The possibility of using thelognormal particle size distribution function and the time dependence of its parametersto reveal the formation routes of the product crystals is analyzed. It is found that thedimensional spectrum of microcrystals has four components, the appearance of whichis associated with different routes of the product formation. The number of distributioncomponents remains unchanged during the prolonged exposure, but their averagecharacteristics have different time dependences. The redistribution of the mass duringrecrystallization, which leads, on the whole, to a decrease in the average crystal size, isexplained by differences in the mobility of the crystal structure of different components. It is concluded that the states of corundum in the newly formed crystals and in thebuildup layer on the particles of the inducing additive are different.
Evaluation of Efficiency of BiologicallyActive Compounds SubcriticalExtraction from the ChamomileFlowers (Chamomilla recutita R.) Growing in the Samara Region
Authors: Павлова Л.В. , Платонов И.А. , Куркин В.А. , Афанасьева П.В. , Новикова Е.А. , Муханова И.М. 
Published: September 2024

Методами УФ-спектроскопии, газовой хроматографии с масс-спектрометрическим детектированием (ГХ-МС), высокоэффективной жидкостной хроматографии (ВЭЖХ) и гравиметрии изучены экстракты биологически активных соединений (БАС) из цветков ромашки аптечной, произрастающей в Самарскойобласти, полученные с использованием субкритической воды, а также водныхрастворов этанола различной концентрации при 150 и 200 °С и 5 МПа. Проведенаидентификация БАС полученных экстрактов с применением реакционной газовой хроматографии.
UV-spectroscopy, gas chromatography / mass spectrometry, high efficiency liquidchromatography and gravimetry methods were employed to study the extracts ofbiologically active substances (BAS) from the flowers of chamomile growing in theSamara region. The extracts were obtained using the subcritical water and aqueoussolutions of ethanol at 150 and 200 °С and 5 MPa. The BAS in the extracts were identifiedusing a reactive gas chromatography.
Crystallization of Polymer Mixtures in theCourse of their Dispersing Using theSEDS Method
Authors: Хабриев И.Ш. , Патрушева М.Н. , Хайрутдинов В.Ф. , Хузаханов Р.М. , Гумеров Ф.М. , Гарипов Р.М. 
Published: September 2024

Приведены результаты экспериментального исследования характеристик фазовых равновесий для систем, включающих толуол, сверхкритический СО2 и сополимеры этилена с винилацетатом (СЭВА). Проведено совместное диспергирование полимерных смесей СЭВА и полиэтилена высокого давления методом SEDSпри 8,0-25,0 МПа и 313, 323 и 333 К. Сравнение процессов плавления и кристаллизации в полученных смесях сополимеров с процессами в смесях того же состава, полученных смешением в расплаве, показало, что использование метода SEDSприводит к увеличению степени кристалличности полимерных смесей.
The results of an experimental study of the characteristics of phase equilibria for thesystems consisting of toluene, supercritical carbon dioxide and ethylene-vinyl acetatecopolymers (EVAC) are presented. The joint dispersing of different EVAC's andpolyethylene using the SEDS method is performed at 8.0-25 MPa and 313, 323 and 333 K. A comparison of melting and crystallization processes in the mixtures of copolymersshows that those obtained by the SEDS method have higher degree of crystallinity ascompared with the mixtures of the same compositions, but obtained by melt mixing.
SOLVATION OF HYDROXYBENZOIC AND HYDROXYCINNAMIC ACIDS IN SUPERCRITICAL CARBON DIOXIDE. PECULIARITIES OF HYDROGEN BONDING WITH A POLAR COSOLVENT
Authors: Гурина Д.Л. , Антипова М.Л. , Одинцова Е.Г. , Петренко В.Е. 
Published: September 2024

С использованием метода классической молекулярной динамики исследована селективная сольватация молекул гидроксибензойных кислот (орто-, мета- и пара-гидроксибензойной, протокатеховой, галловой, сиреневой) и гидроксикоричных кислот (пара-кумаровой, кофейной) полярным сорастворителем (метанол) в среде сверхкритического диоксида углерода при температуре 318 K., плотности 0,7 г/см и концентрации метанола 3,5 мольн. %. Предметом изучения являются локализация сорастворителя в ближайшем окружении растворенного вещества, образование кластеров сорастворителя вокруг растворенного вещества, число и характер водородных связей между молекулами растворенного вещества и сорастворителя, особенности структуры водородносвязанных комплексов, связанные с молекулярным строением растворенного вещества (количеством и расположением гидроксильных групп, замещением гидроксильных водородов метальными фрагментами, наличием этиленовой группы).
Selective solvation of hydroxybenzoic (ortho-, meta- and para-hydroxybenzoic, protocatechuic, gallic, syringic) acids and hydroxycinnamic (para-coumaric, caffeic) acids by polar cosolvent (methanol) in supercritical carbon dioxide at temperature 318 K., solvent density 0.7 g/cm and methanol concentration 3.5 mol. % is studied using molecular dynamics method. The subject of the study is the formation of cosolvent clusters around solute, the solute - cosolvent hydrogen bonding, the structural features of hydrogen-bonded complexes associated with the molecular structure of solute (the number and location of hydroxyl groups, the substitution of hydroxyl hydrogens by methyl groups, and the presence of ethylene group between carboxyl group and benzene ring).
FEATURES OF DISTRIBUTION OF PHOTOACTIVE FILLERS IN POLYMERIC MATRIXES OF DIFFERENT NATURE AT THEIR IMPREGNATION IN THE MEDIUM OF SUPERCRITICAL CARBON DIOXIDE
Authors: Копылов А.С. , Юсупов В.И. , Черкасова А.В. , Шершнев И.В. , Тимашев П.С. , Соловьева А.Б. 
Published: September 2024

Методом флуоресцентной микроскопии изучено распределение фотоактивных соединений (ФАС) в полимерных матрицах, импрегнированных в среде сверхкритического диоксида углерода (СК-С0). Показано, что характер распределения ФАС в полимерах зависит от природы матрицы, степени взаимодействия вводимых молекул ФАС с фрагментами полимера, соответствующих коэффициентов диффузии. Такая информация может оказаться востребованной при разработках функционально ориентированных полимерных систем, импрегнированных ФАС (гетерогенный катализ, фотохромные материалы, медицина).
Fluorescence microscopy was used to study the distribution of photoactive compounds (PAC) in polymer matrixes impregnated in supercritical carbon dioxide (SC-CO). It is shown that the character of the PAC distribution in polymers depends on the nature of the matrix, the degree of interaction of the introduced compounds with polymer fragments, and the sizes of the photoactive compounds corresponding diffusion coefficients. Such information may be in demand for the development of functionally oriented polymer systems impregnated with РАС (heterogeneous catalysis, photochromic materials, medicine).
Preparation of Aerogel Materials Based on Alginate-Chitosan Interpolimer Complex Using Supercritical Fluids
Authors: Вальчук Н.А. , Бровко О.С. , Паламарчук И.А. , Бойцова Т.А. , Боголицын К.Г. , Ивахнов А.Д. , Чухчин Д.Г. , Богданович Н.И. 
Published: September 2024

На основе интерполимерного комплекса альгинат-хитозан с использованием сверхкритических флюидов получены материалы аэрогельного типа с развитой мезопористой структурой. Площадь удельной поверхности образцов достигает 243 м/г, средний размер пор составляет 14,8 нм. Показана возможность использования полученных аэрогелей для создания раневых повязок с включением синтетического лекарственного вещества (антибиотик левомицетин). Установлено, что медленное высвобождение лекарственного вещества (70 % в течение 5 ч) из матрицы аэрогеля обеспечивает пролонгированное и безопасное поступление лекарства к раневой поверхности.
Aerogel materials based on the interpolymer complex of alginate with chitosan using supercritical fluids technology were obtained. It was established that obtained aerogels have a developed mesoporous structure and a specific surface area of up to 243 m2g-1. The possibility of using the aerogels with the inclusion of antibiotic levomycetin as a basis for creating wound dressings was shown.
Oxidation of Acetic Acid with Hydrogen Peroxide in the Aqueous Medium in Supercritical Fluid Conditions
Authors: Мазанов С.В. , Аетов А.У. , Усманов Р.А. , Габитов Р.Р. , Зарипов З.И. , Гумеров Ф.М. 
Published: September 2024

Экспериментально исследовано окисление уксусной кислоты (УК) в 10%-ном водном растворе при 673-748 K и 25 МПа на установках периодического и непрерывного действия. В качестве окислителя использован пероксид водорода. Степень превращения УК определена на основании показателя химического потребления кислорода.
The oxidation of acetic acid (AA) in its 10 % aqueous solution at 673-748 K and 25 MPa is studied in periodic and continuous regimes. Hydrogen peroxide is used as an oxidant. The degree of AA conversion is determined based on the oxygen chemical consumption index.
Perspective Solvents for Lignin Depolimerization: Stability under Supercritical Conditions
Authors: Ивахнов А.Д. , Ульяновский Н.В. , Покрышкин С.А. , Шаврина И.С. , Пиковской И.И. , Косяков Д.С. 
Published: September 2024

Изучена термическая устойчивость в сверхкритических условиях метанола, этанола, 2-пропанола и уксусной кислоты, наиболее пригодных для реализации процессов деполимеризации технических лигнинов с получением низкомолекулярных ароматических соединений. Установлено, что при температуре 450 °С наиболее стабильным является 2-пропанол. Уксусная кислота подвергается практически полному разложению в течение четырех часов, при этом добавка воды существенно повышает ее термическую стабильность. Методом газовой хроматографии-масс-спектрометрии изучен компонентный состав газообразных и жидких продуктов термического разложения растворителей.
Thermal stability under supercritical conditions of methanol, ethanol, 2-propanol and acetic acid, most suitable as solvents in depolymerization of technical lignins to low-molecular aromatic compounds, was studied. Among them at 450 °C 2-propanol is the most stable. Acetic acid is almost completely decomposed within 4 h, while the addition of water significantly increases its thermal stability. Chemical composition of gaseous and liquid products of thermal decomposition of solvents was studied using gas chromatography-mass spectrometry.