bluuebunny/crossref_metadata_2025_split huggingface dataset:
Random lasing in human tissues embedded with organic dyes for cancer diagnosis
Authors: Wang Yu , Duan Zhuojun , Qiu Zhu , Zhang Peng , Wu Jianwei , Zhang Dingke , Xiang Tingxiu 
Published: August 2017

AbstractVarious nanostructures found in biological organisms are often complex and they exhibit unique optical functions. This study surprisingly found that typical random lasing occurs in cancerous human tissues embedded with the nanotextured organic dye 4-(dicyanomethylene)-2-tert-butyl-6-(1,1,7,7- tetramethyljulolidyl-9-enyl)-4H-pyran (DCJTB). Hematoxylin and eosin stain images show that there are more laser resonators in cancerous tissues, caused by a large number of disordered scatters. It is also noteworthy that the random lasing thresholds were found to relate to the tumor malignancy grade. Consequently, the resulting typical random lasing resonators differ between cancerous tissues in different malignancy grades. Further studies are warranted to investigate tissue optical spectroscopy in the field of cancer diagnostics.
Evaluation of Candidate Reference Genes for Quantitative Gene Expression Analysis in Spodoptera exigu
a after Long-time Exposure to Cadmium
Authors: Płachetka-Bożek Anna , Augustyniak Maria 
Published: August 2017

AbstractStudies on the transcriptional control of gene expression play an important role in many areas of biology. Reference genes, which are often referred to as housekeeping genes, such as GAPDH, G3PDH, EF2, RpL7A, RpL10, TUBα and Actin, have traditionally been assumed to be stably expressed in all conditions, and they are frequently used to normalize mRNA levels between different samples in qPCR analysis. However, it is known that the expression of these genes is influenced by numerous factors, such as experimental conditions. The difference in gene expression underlies a range of biological processes, including development, reproduction and behavior. The aim of this study was to show the problems associated with using reference genes in the qPCR technique, in a study on inbred strains of Spodoptera exigua selected toward cadmium resistance. We present and discuss our results and observations, and give some recommendations concerning the use and limitations of housekeeping genes as internal standards, especially in research on insects. Our results suggest that holometabolism and poikilothermia, as well as time since metamorphosis and the level of exposure to the selective factor (cadmium in this case), have a significant effect on the expression of reference genes.
Effect of Genetic Information and Information About Caffeine Content on Caffeine Withdrawal Symptoms
Authors: Mills Llewellyn , Dar-Nimrod Ilan , Colagiuri Ben 
Published: August 2017

AbstractThis study sought to test the effect of genetic information and information about the caffeine content of a beverage on caffeine withdrawal, specifically if: (1) being informed that one has tested positive for a gene related to caffeine withdrawal can produce an exaggerated caffeine withdrawal response during abstinence; (2) belief that one has consumed caffeine leads to a reduction in withdrawal symptoms when no caffeine is consumed. Regular coffee drinkers were given a bogus genetic test and were told either that they had tested positive or negative for a gene related to withdrawal. After 24-hour caffeine abstinence withdrawal symptoms were measured using a self-report caffeine withdrawal scale, and then again after a cup of decaffeinated coffee. Half the participants were told their coffee was caffeinated and half were told truthfully that it was decaffeinated. Participants told the coffee was caffeinated reported a greater reduction in withdrawal symptoms than those told it was decaffeinated. Differing genetic test result information produced no difference in reported withdrawal symptoms. These results indicate that information about the dose of caffeine administered can influence withdrawal symptoms, but that genetic information does not have a universal ability to produce nocebo effects across all sensory and cognitive domains.
Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin
Authors: Gupta Vivek , Mirzaei Mehdi , Gupta Veer Bala , Chitranshi Nitin , Dheer Yogita , Vander Wall Roshana , Abbasi Mojdeh , You Yuyi , Chung Roger , Graham Stuart 
Published: August 2017

AbstractNeuroserpin is a serine protease inhibitor that regulates the activity of plasmin and its activators in the neuronal tissues. This study provides novel evidence of regulatory effect of the neuroserpin on plasmin proteolytic activity in the retina in glaucoma. Human retinal and vitreous tissues from control and glaucoma subjects as well as retinas from experimental glaucoma rats were analysed to establish changes in plasmin and neuroserpin activity. Neuroserpin undergoes oxidative inactivation in glaucoma which leads to augmentation of plasmin activity. Neuroserpin contains several methionine residues in addition to a conserved reactive site methionine and our study revealed enhanced oxidation of Met residues in the serpin under glaucoma conditions. Met oxidation was associated with loss of neuroserpin inhibitory activity and similar findings were observed in the retinas of superoxide dismutase (SOD) mutant mice that have increased oxidative stress. Treatment of purified neuroserpin with H2O2 further established that Met oxidation inversely correlated with its plasmin inhibitory activity. Dysregulation of the plasmin proteolytic system associated with increased degradation of the extracellular matrix (ECM) proteins in the retina. Collectively, these findings delineate a novel molecular basis of plasmin activation in glaucoma and potentially for other neuronal disorders with implications in disease associated ECM remodelling.
Emotions alter muscle proprioceptive coding of movements in humans
Authors: Ackerley Rochelle , Aimonetti Jean-Marc , Ribot-Ciscar Edith 
Published: August 2017

AbstractEmotions can evoke strong reactions that have profound influences, from gross changes in our internal environment to small fluctuations in facial muscles, and reveal our feelings overtly. Muscles contain proprioceptive afferents, informing us about our movements and regulating motor activities. Their firing reflects changes in muscle length, yet their sensitivity can be modified by the fusimotor system, as found in animals. In humans, the sensitivity of muscle afferents is modulated by cognitive processes, such as attention; however, it is unknown if emotional processes can modulate muscle feedback. Presently, we explored whether muscle afferent sensitivity adapts to the emotional situation. We recorded from single muscle afferents in the leg, using microneurography, and moved the ankle joint of participants, while they listened to evocative classical music to induce sad, neutral, or happy emotions, or sat passively (no music). We further monitored their physiological responses using skin conductance, heart rate, and electromyography measures. We found that muscle afferent firing was modified by the emotional context, especially for sad emotions, where the muscle spindle dynamic response increased. We suggest that this allows us to prime movements, where the emotional state prepares the body for consequent behaviour-appropriate reactions.
Antibacterial activity and effect on gingival cells of microwave-pulsed non-thermal atmospheric pressure plasma in artificial saliva
Authors: Seo Sang-Hee , Han Ihn , Lee Han Seol , Choi Jin Joo , Choi Eun Ha , Kim Kyoung-Nam , Park Gyungsoon , Kim Kwang-Mahn 
Published: August 2017

AbstractAlthough various oral pathogens are inactivated by non-thermal atmospheric pressure plasma (NTAPP), the in vivo effects of NTAPP are poorly understood. The first aim of this study was to examine the antibacterial activity of microwave-pulsed NTAPP against Staphylococcus aureus in artificial saliva to mimic oral environmental conditions. The second aim was to determine the influence of microwave-pulsed NTAPP on human gingival fibroblasts (HGFs). The microwave-pulsed NTAPP reduced bacterial viability (as measured by colony forming units [CFU]) to a greater extent in artificial saliva than in saline. Extending the post-treatment incubation time increased bacterial inactivation in artificial saliva compared to saline. HGFs viability was unaffected by microwave-pulsed NTAPP for bacterial inactivation. Rather, HGFs proliferation increased after a 5-min microwave-pulsed NTAPP. Less tumor necrosis factor alpha was released by microwave-pulsed NTAPP-treated HGFs stimulated with lipopolysaccharide (LPS) than by untreated, LPS-stimulated HGFs; thus, plasma appeared to suppress the inflammatory response. Our study suggests that microwave-pulsed NTAPP may have stronger in vivo antibacterial activity than in vitro activity, and that microwave-pulsed NTAPP may have the additional advantage of suppressing gingival inflammatory responses.
Active Components of Leptospira Outer Membrane Protein LipL32 to Toll-Like Receptor 2
Authors: Hsu Shen-Hsing , Hung Cheng-Chieh , Chang Ming-Yang , Ko Yi-Ching , Yang Huang-Yu , Hsu Hsiang-Hao , Tian Ya-Chung , Chou Li-Fang , Pan Rong-Long , Tseng Fan-Gang , Yang Chih-Wei 
Published: August 2017

AbstractProteins belonging to the toll-like receptor (TLR) family, particularly TLR2, are the major components of innate immunity against Leptospira infection. The ligands for TLR2 harbor several conserved patterns such as lipidation molecules, leucine-rich repeat (LRR) domains, TLR2 binding motifs, and TLR2 binding structure. In Leptospira, LipL32 interacts with TLR2 on human kidney cells concomitantly stimulating inflammatory responses. However, the binding mechanism of LipL32 to TLR2 is unknown. The computational prediction suggests that β1β2, loop-α3-loop, and α4 domains of LipL32 play vital roles in LipL32-TLR2 complex formation. To test these predictions, protein truncation experiments revealed that LipL32ΔNβ1β2 significantly decreased the affinity to TLR2 while LipL32ΔCα4 slightly reduced it. Interestingly, LipL32ΔCenα3 retained affinity to TLR2 in the absence of Ca2+ ions, indicating that Cenα3 play a role preventing the interaction between LipL32 and TLR2. Furthermore, the critical residues of LipL32 involved in interacting with TLR2 suggested that V35S, L36S and L263S variants significantly decreased the affinity to TLR2. The results further confirm that LipL32 interacts with TLR2 through Nβ1β2 and Cα4 domains of LipL32 as well as LipL32-TLR2 complex formation results from hydrophobic interactions. This study provides a detailed mechanism of the interaction between LipL32 and TLR2 and the residues involved in complex formation.
Percolation Phase Transition of Surface Air Temperature Networks: A new test bed for El Niño/La Niña simulations
Authors: Hua Lijuan , Lu Zhenghui , Yuan Naiming , Chen Lin , Yu Yongqiang , Wang Lu 
Published: August 2017

AbstractIn this work, we studied the air-sea interaction over the tropical central eastern Pacific from a new perspective, climate network. The surface air temperatures over the tropical Pacific were constructed as a network, and the nodes within this network were linked if they have a similar temporal varying pattern. Using three different reanalysis datasets, we verified the percolation phase transition. That is, when the influences of El Niño/La Niña are strong enough to isolate more than 48% of the nodes, the network may abruptly be divided into many small pieces, indicating a change of the network state. This phenomenon was reproduced successfully by a coupled general circulation model, Flexible Global Ocean-Atmosphere-Land System Model Spectral Version 2, but another model, Flexible Global Ocean-Atmosphere-Land System Model Grid-point Version 2, failed. As both models have the same oceanic component, but are with different atmospheric components, the improperly used atmospheric component should be responsible for the missing of the percolation phase transition. Considering that this new phenomenon is only recently noticed, current state-of-the-art models may ignore this process and induce unrealistic simulations. Accordingly, percolation phase transition is proposed as a new test bed, which deserves more attention in the future.
Reference miRNAs for colorectal cancer: analysis and verification of current data
Authors: Danese E. , Minicozzi A. M , Benati M. , Paviati E. , Lima-Oliveira G. , Gusella M. , Pasini F. , Salvagno G. L , Montagnana M. , Lippi G. 
Published: August 2017

AbstractMicroRNAs (miRNAs) hold great promise in cancer research. The use of appropriate reference miRNAs for normalization of qPCR data is crucial for accurate expression analysis. We present here analysis and verification of current data, proposing a workflow strategy for identification of reference miRNAs in colorectal cancer (CRC). We performed a systematic review of studies aimed to identify stable reference miRNAs in CRC through high-throughput screening. Among the candidate miRNAs selected from the literature we excluded those predicted to target oncogenes or tumor suppressor gene. We then assessed the expression levels of the remaining candidates in exosomes, plasma and tissue samples from CRC patients and healthy controls. The expression stability was evaluated by box-plot, ∆Cq analysis, NormFinder and BestKeeper statistical algorithms. The effects of normalisers on the relative quantification of the oncogenic miR-1290 was also assessed. Our results consistently showed that different combinations of miR-520d, miR-1228 and miR-345 provided the most stably expressed reference miRNAs in the three biological matrices. We identified suitable reference miRNAs for future miRNA expression studies in exosomes plasma and tissues CRC samples. We also provided a novel conceptual framework that overcome the need of performing ex novo identification of suitable reference genes in single experimental systems.
Top canopy nitrogen allocation linked to increased grassland carbon uptake in stands of varying species richness
Authors: Milcu Alexandru , Gessler Arthur , Roscher Christiane , Rose Laura , Kayler Zachary , Bachmann Dörte , Pirhofer-Walzl Karin , Zavadlav Saša , Galiano Lucia , Buchmann Tina , Scherer-Lorenzen Michael , Roy Jacques 
Published: August 2017

AbstractModels predict that vertical gradients of foliar nitrogen (N) allocation, increasing from bottom to top of plant canopies, emerge as a plastic response to optimise N utilisation for carbon assimilation. While this mechanism has been well documented in monocultures, its relevance for mixed stands of varying species richness remains poorly understood. We used 21 naturally assembled grassland communities to analyse the gradients of N in the canopy using N allocation coefficients (K
N
) estimated from the distribution of N per foliar surface area (KN-F) and ground surface area (KN-G). We tested whether: 1) increasing plant species richness leads to more pronounced N gradients as indicated by higher K
N
-values, 2) K
N
is a good predictor of instantaneous net ecosystem CO2 exchange and 3) functional diversity of leaf N concentration as estimated by Rao’s Q quadratic diversity metric is a good proxy of K
N
. Our results show a negative (for KN-G) or no relationship (for KN-F) between species richness and canopy N distribution, but emphasize a link (positive relationship) between more foliar N per ground surface area in the upper layers of the canopy (i.e. under higher KN-G) and ecosystem CO2 uptake. Rao’s Q was not a good proxy for either K
N
.
Analysis on the Composition/structure and Lacquering Techniques of the Coffin of Emperor Qianlong Excavated from the Eastern Imperial Tombs
Authors: Hao Xinying , Wu Hao , Zhao Yang , Tong Tong , Li Xiaoyuan , Yang Cui , Tang Yun , Shen Xinyu , Tong Hua 
Published: August 2017

AbstractThis article presents the results of an investigation on the coffin of Emperor Qianlong excavated from the Eastern Imperial Tombs of the Qing Dynasty in Zunhua, China. The composition, structure and lacquering techniques used in the manufacturing process were analyzed in this project. Stereoscopic Microscopy, SEM-EDS, XRD, FTIR, Raman, Double-shot Py-GC/MS were used as scientific analytical methods. The results show that the structure of the coffin body consists of a wooden body layer, a lacquer ash layer and a lacquer film pigment layer. The lacquer ash layer consists of nine stucco layers and ten fiber layers on top of each other in an alternating order. The lacquer film pigment layer consists of mineral pigments, lacquer sap, animal gelatin, drying oil, quartz sand and proteinaceous materials. Pigments used in the lacquer film include calcite white, carbon black, cinnabar red and gold. The presence of three distinctive catechols along with the other catechols’ and phenols’ profiles in the lacquer film matrix clearly indicate the species of the lacquer tree was Rhus. Vernicifera. Several distinctive lacquering techniques that improved the coffin body’s stability and mechanical strength were identified in the investigation, including the “wan lacquering”, “painting lacquer above the gold” and “Jin Jiao”.
Risk Factors For Radiation-Induced Skin Ulceration in Percutaneous Coronary Interventions of Chronic Total Occluded Lesions: A 2-Year Observational Study
Authors: Lai Chi-Cheng , Wei Kai-Che , Chen Wen-Yee , Mar Guang-Yuan , Wang Wen-Hwa , Wu Chieh-Shan , Tseng Ching-Jiunn , Yang Kuo-Chung , Chen Lee-Wei , Liu Chun-Peng 
Published: August 2017

AbstractRelationship between radiation-induced skin ulceration (RSU) and variables in percutaneous coronary interventions (PCI) was rarely reported. RSU is a severe complication in PCIs, especially for chronic total occlusion (CTO) lesions. We investigated the RSUs and their risk factors in patients receiving CTO PCIs over a 2-year period. Data were analyzed using chi-square tests, t-tests and receiver operating characteristic (ROC) curve. Of 238 patients, 11 patients (4.6%) had RSUs all at right upper back. RSUs were significantly associated with use of left anterior oblique (LAO) views (100% vs. 47.1%, p < 0.001), retrograde techniques (36.3% vs. 7.9%, p = 0.012), or a procedure time (PT) defined as a time duration between the first and last angiograms of > 120, 180, or 240 minutes (p < 0.05). ROC analysis showed a long PT was an accurate predictor of RSUs (AUC = 0.88; p < 0.001) at a cut-off of 130 minutes (sensitivity = 0.91, specificity = 0.81). The results showed risk factors for RSUs containing use of large LAO views, retrograde techniques, and prolonged PTs. This study suggests that, to minimize RSU, interventionalists should limit PT to roughly 2 hours in fixed LAO views.
Targeted insertion of an anti-CD2 monoclonal antibody transgene into the GGTA1 locus in pigs using FokI-dCas9
Authors: Nottle Mark B. , Salvaris Evelyn J. , Fisicaro Nella , McIlfatrick Stephen , Vassiliev Ivan , Hawthorne Wayne J. , O’Connell Philip J. , Brady Jamie L. , Lew Andrew M. , Cowan Peter J. 
Published: August 2017

AbstractXenotransplantation from pigs has been advocated as a solution to the perennial shortage of donated human organs and tissues. CRISPR/Cas9 has facilitated the silencing of genes in donor pigs that contribute to xenograft rejection. However, the generation of modified pigs using second-generation nucleases with much lower off-target mutation rates than Cas9, such as FokI-dCas9, has not been reported. Furthermore, there have been no reports on the use of CRISPR to knock protective transgenes into detrimental porcine genes. In this study, we used FokI-dCas9 with two guide RNAs to integrate a 7.1 kilobase pair transgene into exon 9 of the GGTA1 gene in porcine fetal fibroblasts. The modified cells lacked expression of the αGal xenoantigen, and secreted an anti-CD2 monoclonal antibody encoded by the transgene. PCR and sequencing revealed precise integration of the transgene into one allele of GGTA1, and a small deletion in the second allele. The cells were used for somatic cell nuclear transfer to generate healthy male knock-in piglets, which did not express αGal and which contained anti-CD2 in their serum. We have therefore developed a versatile high-fidelity system for knocking transgenes into the pig genome for xenotransplantation purposes.
Molecular characterization of two novel echovirus 18 recombinants associated with hand-foot-mouth disease
Authors: Zhang Haihao , Zhao Yilin , Liu Hongbo , Sun Hao , Huang Xiaoqin , Yang Zhaoqing , Ma Shaohui 
Published: August 2017

AbstractHuman echovirus 18 (E-18) is a member of the enterovirus B species. To date, sixteen full-length genome sequences of E-18 are available in the GenBank database. In this study, we describe the complete genomic characterization of two E-18 strains isolated in Yunnan, China. Pairwise comparisons of the nucleotide sequences and the deduced amino acid sequences revealed that the two Yunnan E-18 strains had 87.5% nucleotide identity and 96.3–96.5% amino acid identity with the Chinese strain. Phylogenetic and bootscanning analyses revealed the two E-18 strains had the highest identity with other several EV-B serotypes than the other E-18 strains in the P3 coding region, especially, 3B region of the Swine Vesicular disease virus (SVDV) strain HK70, indicated that frequent intertypic recombination might have occurred in the two Yunnan strains. This study contributes the complete genome sequences of E-18 to the GenBank database and provides valuable information on the molecular epidemiology of E-18 in China.
Body mass index trajectories in the first two years and subsequent childhood cardio-metabolic outcomes: a prospective multi-ethnic Asian cohort study
Authors: Aris Izzuddin M. , Chen Ling-Wei , Tint Mya Thway , Pang Wei Wei , Soh Shu E. , Saw Seang-Mei , Shek Lynette Pei-Chi , Tan Kok-Hian , Gluckman Peter D. , Chong Yap-Seng , Yap Fabian , Godfrey Keith M. , Kramer Michael S. , Lee Yung Seng 
Published: August 2017

AbstractWe investigated body mass index (BMI) trajectories in the first 2 years of life in 1170 children from an Asian mother-offspring cohort in Singapore, and examined their predictors and associations with childhood cardio-metabolic risk measures at 5 years. Latent class growth mixture modelling analyses were performed to identify distinct BMI z-score (BMIz) trajectories. Four trajectories were identified: 73.2%(n = 857) of the children showed a normal BMIz trajectory, 13.2%(n = 155) a stable low-BMIz trajectory, 8.6%(n = 100) a stable high-BMIz trajectory and 5.0%(n = 58) a rapid BMIz gain after 3 months trajectory. Predictors of the stable high-BMIz and rapid BMIz gain trajectories were pre-pregnancy BMI, gestational weight gain, Malay and Indian ethnicity, while predictors of stable low-BMIz trajectory were preterm delivery and Indian ethnicity. At 5 years, children with stable high-BMIz or rapid BMIz gain trajectories had increased waist-to-height ratios [B(95%CI) 0.02(0.01,0.03) and 0.03(0.02,0.04)], sum of skinfolds [0.42(0.19,0.65) and 0.70(0.36,1.03)SD units], fat-mass index [0.97(0.32,1.63)SD units] and risk of obesity [relative risk 3.22(1.73,6.05) and 2.56 (1.19,5.53)], but not higher blood pressure. BMIz trajectories were more predictive of adiposity at 5 years than was BMIz at 2 years. Our findings on BMIz trajectories in the first 2 years suggest important ethnic-specific differences and impacts on later metabolic outcomes.