bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transmission of Induced Chromosomal Aberrations through Successive Mitotic Divisions in Human Lymphocytes after In Vitro and ﻿In﻿ Vivo Radiation
Authors: Kaddour Akram , Colicchio Bruno , Buron Diane , El Maalouf Elie , Laplagne Eric , Borie Claire , Ricoul Michelle , Lenain Aude , Hempel William M. , Morat Luc , Al Jawhari Mustafa , Cuceu Corina , Heidingsfelder Leonhard , Jeandidier Eric , Deschênes Georges , Dieterlen Alain , El May Michèle , Girinsky Theodore , Bennaceur-Griscelli Annelise , Carde Patrice , Sabatier Laure , M’kacher Radhia 
Published: June 2017

AbstractThe mechanisms behind the transmission of chromosomal aberrations (CA) remain unclear, despite a large body of work and major technological advances in chromosome identification. We reevaluated the transmission of CA to second- and third-division cells by telomere and centromere (TC) staining followed by M-FISH. We scored CA in lymphocytes of healthy donors after in vitro irradiation and those of cancer patients treated by radiation therapy more than 12 years before. Our data demonstrate, for the first time, that dicentric chromosomes (DCs) decreased by approximately 50% per division. DCs with two centromeres in close proximity were more efficiently transmitted, representing 70% of persistent DCs in ≥M3 cells. Only 1/3 of acentric chromosomes (ACs), ACs with four telomeres, and interstitial ACs, were paired in M2 cells and associated with specific DCs configurations. In lymphocytes of cancer patients, 82% of detected DCs were characterized by these specific configurations. Our findings demonstrate the high stability of DCs with two centromeres in close proximity during cell division. The frequency of telomere deletion increased during cell cycle progression playing an important role in chromosomal instability. These findings could be exploited in the follow-up of exposed populations.
Changes in novel haematological parameters following thermal injury: A prospective observational cohort study
Authors: Dinsdale R. J. , Devi A. , Hampson P. , Wearn C. M. , Bamford A. L. , Hazeldine J. , Bishop J. , Ahmed S. , Watson C. , Lord J. M. , Moiemen N. , Harrison P. 
Published: June 2017

AbstractThe mortality caused by sepsis is high following thermal injury. Diagnosis is difficult due to the ongoing systemic inflammatory response. Previous studies suggest that cellular parameters may show promise as diagnostic markers of sepsis. The aim of this study was to evaluate the effect of thermal injury on novel haematological parameters and to study their association with clinical outcomes. Haematological analysis was performed using a Sysmex XN-1000 analyser on blood samples acquired on the day of the thermal injury to 12 months post-injury in 39 patients (15–95% TBSA). Platelet counts had a nadir at day 3 followed by a rebound thrombocytosis at day 21, with nadir values significantly lower in septic patients. Measurements of extended neutrophil parameters (NEUT-Y and NEUT-RI) demonstrated that septic patients had significantly higher levels of neutrophil nucleic acid content. A combination of platelet impedance count (PLT-I) and NEUT-Y at day 3 post-injury exhibited good discriminatory power for the identifying septic patients (AUROC = 0.915, 95% CI [0.827, 1.000]). Importantly, the model had improved performance when adjusted for mortality with an AUROC of 0.974 (0.931, 1.000). A combination of PLT-I and NEUT-Y show potential for the early diagnosis of sepsis post-burn injury. Importantly, these tests can be performed rapidly and require a small volume of whole blood highlighting their potential utility in clinical practice.
APOE gene polymorphism in long-lived individuals from a central China population
Authors: Liu Guodong , Liu Xiang , Yu Pulin , Wang Qi , Wang Hua , Li Chenfang , Ye Guangming , Wu Xiaoling , Tan Chunling 
Published: June 2017

AbstractPrevious studies from European and East Asian cohorts reported conflicting results over whether and how the frequencies of the three common alleles, ε2, ε3 and ε4, of the apolioprotein E gene (APOE), in long-lived individuals differ from those in younger age groups. This study was the first to analyse these frequencies of long-lived individuals from central China. Genotyping of APOE alleles and genotypes was carried out in 70 long-lived individuals and 204 younger controls. No difference in the frequency of any APOE allele or genotype was found between the long-lived participants and their younger controls, but the long-lived group seemed to have a higher ε4 frequency (15.71%) than the 24–50 and 51–75 age groups (10.2% and 11.32%, P > 0.05). Notably, when compared with two other Chinese studies, the central China long-lived group had a higher ε4 frequency than its southern and eastern China counterparts (15.71% vs. 2.82% and 2.54%, P < 0.05). It is not clear to what extent population substructure or lifestyles contributed to these divergent findings. A clear understanding of the contribution of APOE polymorphisms to longevity in the Han Chinese population may be achieved only through large scale studies with participants from well-defined regional clusters.
Internal gradient distributions: A susceptibility-derived tensor delivering morphologies by magnetic resonance
Authors: Álvarez Gonzalo A. , Shemesh Noam , Frydman Lucio 
Published: June 2017

AbstractNuclear magnetic resonance is a powerful tool for probing the structures of chemical and biological systems. Combined with field gradients it leads to NMR imaging (MRI), a widespread tool in non-invasive examinations. Sensitivity usually limits MRI’s spatial resolution to tens of micrometers, but other sources of information like those delivered by constrained diffusion processes, enable one extract morphological information down to micron and sub-micron scales. We report here on a new method that also exploits diffusion – isotropic or anisotropic– to sense morphological parameters in the nm-mm range, based on distributions of susceptibility-induced magnetic field gradients. A theoretical framework is developed to define this source of information, leading to the proposition of internal gradient-distribution tensors. Gradient-based spin-echo sequences are designed to measure these new observables. These methods can be used to map orientations even when dealing with unconstrained diffusion, as is here demonstrated with studies of structured systems, including tissues.
In–situ XRD and EDS method study on the oxidation behaviour of Ni–Cu sulphide ore
Authors: Li Guangshi , Cheng Hongwei , Xiong Xiaolu , Lu Xionggang , Xu Cong , Lu Changyuan , Zou Xingli , Xu Qian 
Published: June 2017

AbstractThe oxidation mechanism of sulfides is the key issue during the sulphide–metallurgy process. In this study, the phase transformation and element migration were clearly demonstrated by in–situ laboratory–based X–ray diffraction (XRD) and energy–dispersive X–ray spectroscopy (EDS), respectively. The reaction sequence and a four–step oxidation mechanism were proposed and identified. The elemental distribution demonstrated that at a low temperature, the Fe atoms diffused outward and the Ni/Cu atoms migrated toward the inner core, whereas the opposite diffusion processes were observed at a higher temperature. Importantly, the unique visual presentation of the oxidation behaviour provided by the combination of in–situ XRD and EDS might be useful for optimising the process parameters to improve the Ni/Cu extraction efficiency during Ni–Cu sulphide metallurgy.
Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
Authors: Krause Susanne , Bremges Andreas , Münch Philipp C. , McHardy Alice C. , Gescher Johannes 
Published: June 2017

AbstractThis study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.
Single-point Mutation of an Histidine-aspartic Domain-containing Gene involving in Chloroplast Ribosome Biogenesis Leads to White Fine Stripe Leaf in Rice
Authors: Ge Changwei , Wang Li , Ye Weijun , Wu Liwen , Cui Yongtao , Chen Ping , Pan Jiangjie , Zhang Dong , Hu Jiang , Zeng Dali , Dong Guojun , Qian Qian , Guo Longbiao , Xue Dawei 
Published: June 2017

AbstractPlant leaves are a crucial organ associated closely with chloroplast development, photosynthesis rate and crop productivity. In this study, a white fine stripe leaf 1 (wfsl1) mutant was isolated and characterized from the japonica rice Zhonghua11 (ZH11) after ethyl methanesulfonate mutagenesis. The wfsl1 displayed white fine stripe leaves since tillering stage and abnormal chloroplast structure. Map-based cloning and Bioinformatic analysis indicated that WFSL1 on chromosome 1 contains an “A” to “T” substitution in protein coding region, and encodes a putative metal-dependent phosphohydrolase with HD domain at the N-terminus. WFSL1 was targeted to the chloroplasts and had higher expression in mature leaves and sheaths. RNA-seq analysis revealed that chloroplast development and photosynthesis genes were significantly affected in wfsl1 plants. Levels of WFSL1 and chloroplast encoded proteins were decreased in wfsl1 mutants via western blot analysis. Compared with WT, wfsl1 exhibits lower Chl content and defective in biogenesis of chloroplast ribosomes, which resulted in reduced grain yield. Taken together, our results show that WFSL1 is critical for chloroplast development, ribosome biogenesis, and light energy utilization, finally affects grain yield.
Arctic sea ice melt leads to atmospheric new particle formation
Authors: Dall´Osto M. , Beddows D. C. S. , Tunved P. , Krejci R. , Ström J. , Hansson H.-C. , Yoon Y. J. , Park Ki-Tae , Becagli S. , Udisti R. , Onasch T. , O´Dowd C. D. , Simó R. , Harrison Roy M. 
Published: June 2017

AbstractAtmospheric new particle formation (NPF) and growth significantly influences climate by supplying new seeds for cloud condensation and brightness. Currently, there is a lack of understanding of whether and how marine biota emissions affect aerosol-cloud-climate interactions in the Arctic. Here, the aerosol population was categorised via cluster analysis of aerosol size distributions taken at Mt Zeppelin (Svalbard) during a 11 year record. The daily temporal occurrence of NPF events likely caused by nucleation in the polar marine boundary layer was quantified annually as 18%, with a peak of 51% during summer months. Air mass trajectory analysis and atmospheric nitrogen and sulphur tracers link these frequent nucleation events to biogenic precursors released by open water and melting sea ice regions. The occurrence of such events across a full decade was anti-correlated with sea ice extent. New particles originating from open water and open pack ice increased the cloud condensation nuclei concentration background by at least ca. 20%, supporting a marine biosphere-climate link through sea ice melt and low altitude clouds that may have contributed to accelerate Arctic warming. Our results prompt a better representation of biogenic aerosol sources in Arctic climate models.
Social odours covary with bacterial community in the anal secretions of wild meerkats
Authors: Leclaire Sarah , Jacob Staffan , Greene Lydia K. , Dubay George R. , Drea Christine M. 
Published: June 2017

AbstractThe fermentation hypothesis for animal signalling posits that bacteria dwelling in an animal’s scent glands metabolize the glands’ primary products into odorous compounds used by the host to communicate with conspecifics. There is, however, little evidence of the predicted covariation between an animal’s olfactory cues and its glandular bacterial communities. Using gas chromatography-mass spectrometry, we first identified the volatile compounds present in ‘pure’ versus ‘mixed’ anal-gland secretions (‘paste’) of adult meerkats (Suricata suricatta) living in the wild. Low-molecular-weight chemicals that likely derive from bacterial metabolism were more prominent in mixed than pure secretions. Focusing thereafter on mixed secretions, we showed that chemical composition varied by sex and was more similar between members of the same group than between members of different groups. Subsequently, using next-generation sequencing, we identified the bacterial assemblages present in meerkat paste and documented relationships between these assemblages and the host’s sex, social status and group membership. Lastly, we found significant covariation between the volatile compounds and bacterial assemblages in meerkat paste, particularly in males. Together, these results are consistent with a role for bacteria in the production of sex- and group-specific scents, and with the evolution of mutualism between meerkats and their glandular microbiota.
Genome-wide association study of outcrossing in cytoplasmic male sterile lines of rice
Authors: Guo Liang , Qiu Fulin , Gandhi Harish , Kadaru Suresh , De Asis Erik Jon , Zhuang Jieyun , Xie Fangming 
Published: June 2017

AbstractStigma exsertion and panicle enclosure of male sterile lines are two key determinants of outcrossing in hybrid rice seed production. Based on 43,394 single nucleotide polymorphism markers, 217 cytoplasmic male sterile lines were assigned into two subpopulations and a mixed-group where the linkage disequilibrium decay distances varied from 975 to 2,690 kb. Genome-wide association studies (GWAS) were performed for stigma exsertion rate (SE), panicle enclosure rate (PE) and seed-setting rate (SSR). A total of 154 significant association signals (P < 0.001) were identified. They were situated in 27 quantitative trait loci (QTLs), including 11 for SE, 6 for PE, and 10 for SSR. It was shown that six of the ten QTLs for SSR were tightly linked to QTLs for SE or/and PE with the expected allelic direction. These QTL clusters could be targeted to improve the outcrossing of female parents in hybrid rice breeding. Our study also indicates that GWAS-base QTL mapping can complement and enhance previous QTL information for understanding the genetic relationship between outcrossing and its related traits.
Conductive Cellulose Composites with Low Percolation Threshold for 3D Printed Electronics
Authors: Park Jae Sung , Kim Taeil , Kim Woo Soo 
Published: June 2017

AbstractWe are reporting a 3D printable composite paste having strong thixotropic rheology. The composite has been designed and investigated with highly conductive silver nanowires. The optimized electrical percolation threshold from both simulation and experiment is shown from 0.7 vol. % of silver nanowires which is significantly lower than other composites using conductive nano-materials. Reliable conductivity of 1.19 × 102 S/cm has been achieved from the demonstrated 3D printable composite with 1.9 vol. % loading of silver nanowires. Utilizing the high conductivity of the printable composites, 3D printing of designed battery electrode pastes is demonstrated. Rheology study shows superior printability of the electrode pastes aided by the cellulose’s strong thixotropic rheology. The designed anode, electrolyte, and cathode pastes are sequentially printed to form a three-layered lithium battery for the demonstration of a charging profile. This study opens opportunities of 3D printable conductive materials to create printed electronics with the next generation additive manufacturing process.
Selective inhibition of small-diameter axons using infrared light
Authors: Lothet Emilie H. , Shaw Kendrick M. , Lu Hui , Zhuo Junqi , Wang Yves T. , Gu Shi , Stolz Donna B. , Jansen E. Duco , Horn Charles C. , Chiel Hillel J. , Jenkins Michael W. 
Published: June 2017

AbstractNovel clinical treatments to target peripheral nerves are being developed which primarily use electrical current. Recently, infrared (IR) light was shown to inhibit peripheral nerves with high spatial and temporal specificity. Here, for the first time, we demonstrate that IR can selectively and reversibly inhibit small-diameter axons at lower radiant exposures than large-diameter axons. We provide a mathematical rationale, and then demonstrate it experimentally in individual axons of identified neurons in the marine mollusk Aplysia californica, and in axons within the vagus nerve of a mammal, the musk shrew Suncus murinus. The ability to selectively, rapidly, and reversibly control small-diameter sensory fibers may have many applications, both for the analysis of physiology, and for treating diseases of the peripheral nervous system, such as chronic nausea, vomiting, pain, and hypertension. Moreover, the mathematical analysis of how IR affects the nerve could apply to other techniques for controlling peripheral nerve signaling.
Molecular Phylogenies indicate a Paleo-Tibetan Origin of Himalayan Lazy Toads (Scutiger)
Authors: Hofmann Sylvia , Stöck Matthias , Zheng Yuchi , Ficetola Francesco G. , Li Jia-Tang , Scheidt Ulrich , Schmidt Joachim 
Published: June 2017

AbstractThe Himalaya presents an outstanding geologically active orogen and biodiversity hotspot. However, our understanding of the historical biogeography of its fauna is far from comprehensive. Many taxa are commonly assumed to have originated from China-Indochina and dispersed westward along the Himalayan chain. Alternatively, the “Tibetan-origin hypothesis” suggests primary diversification of lineages in Paleo-Tibet, and secondary diversification along the slopes of the later uplifted Greater Himalaya. We test these hypotheses in high-mountain megophryid anurans (Scutiger). Extensive sampling from High Asia, and analyses of mitochondrial (2839 bp) and nuclear DNA (2208 bp), using Bayesian and Maximum likelihood phylogenetics, suggest that the Himalayan species form a distinct clade, possibly older than those from the eastern Himalaya-Tibet orogen. While immigration from China-Indochina cannot be excluded, our data may indicate that Himalayan Scutiger originated to the north of the Himalaya by colonization from Paleo-Tibet and then date back to the Oligocene. High intraspecific diversity of Scutiger implies limited migration across mountains and drainages along the Himalaya. While our study strengthens support for a “Tibetan-origin hypothesis”, current sampling (10/22 species; 1 revalidated﻿: S. occidentalis) remains insufficient to draw final conclusions on Scutiger but urges comparative phylogeographers to test alternative, geologically supported hypotheses for a true future understanding of Himalayan biogeography.
Amyloid β peptides overexpression in retinal pigment epithelial cells via AAV-mediated gene transfer mimics AMD-like pathology in mice
Authors: Prasad Tuhina , Zhu Ping , Verma Amrisha , Chakrabarty Paramita , Rosario Awilda M. , Golde Todd E. , Li Qiuhong 
Published: June 2017

AbstractAge-related macular degeneration (AMD) is a progressive retinal neurodegenerative disorder characterized by extracellular deposits known as drusen. A major constituent of drusen deposits are Alzheimer disease-associated amyloid β (Aβ) peptides. To understand the etiology of Aβ proteostasis in AMD, we delivered recombinant adeno-associated virus (AAV) encoding Aβ42 and Aβ40 peptides fused to BRI2 protein by intraocular injection in C57BL/6J mice. Endogenous protease cleavage of such constructs leads to production of secreted Aβ42 and Aβ40 respectively. We demonstrate that overexpression of secreted Aβ40 or Aβ42 resulted in dramatic induction of drusen-like deposits by 2 months’ post-injection. These drusen-like deposits were immunopositive for Aβ and complement proteins but did not stain for conventional amyloid dyes, such as Thioflavin S. Both injected cohorts showed gliosis and degenerative changes, though ERG responses were minimally affected. Intriguingly, simultaneous overexpression of BRI-Aβ40 or BRI-Aβ42 together resulted in dose-dependent and cumulative changes reminiscent of AMD type pathology - drusen-like deposits, severe reduction in ERG responses, photoreceptor cell loss and gliosis. Here, we have established a physiological model of Aβ containing deposits in wild-type mice that recapitulates major retinal pathophysiological features of AMD and will be instrumental in mechanistic understanding and development of therapeutic strategies against AMD.
Growth of high-quality Bi2Sr2 CaCu2O8+δ
whiskers and electrical properties of resulting exfoliated flakes
Authors: Jindal Apoorv , Jangade Digambar A. , Kumar Nikhil , Vaidya Jaykumar , Das Ipsita , Bapat Rudheer , Parmar Jayesh , Chalke Bhagyashree A. , Thamizhavel Arumugam , Deshmukh Mandar M. 
Published: June 2017

AbstractIn this work, we demonstrate a simple technique to grow high-quality whiskers of Bi2 Sr2 CaCu2 O8+δ
– a high T
c
superconductor. Structural analysis shows the single-crystalline nature of the grown whiskers. To probe electrical properties, we exfoliate these whiskers into thin flakes (~50 nm thick) using the scotch-tape technique and develop a process to realize good electrical contacts. We observe a superconducting critical temperature, T
c
, of 86 K. We map the evolution of the critical current as a function of temperature. With 2-D materials emerging as an exciting platform to study low-dimensional physics, our work paves the way for future studies on two-dimensional high-T
c
superconductivity.
Vegetation changes and land surface feedbacks drive shifts in local temperatures over Central Asia
Authors: Yuan Xiuliang , Wang Wenfeng , Cui Junjie , Meng Fanhao , Kurban Alishir , De Maeyer Philippe 
Published: June 2017

AbstractVegetation changes play a vital role in modifying local temperatures although, until now, the climate feedback effects of vegetation changes are still poorly known and large uncertainties exist, especially over Central Asia. In this study, using remote sensing and re-analysis of existing data, we evaluated the impact of vegetation changes on local temperatures. Our results indicate that vegetation changes have a significant unidirectional causality relationship with regard to local temperature changes. We found that vegetation greening over Central Asia as a whole induced a cooling effect on the local temperatures. We also found that evapotranspiration (ET) exhibits greater sensitivity to the increases of the Normalized Difference Vegetation Index (NDVI) as compared to albedo in arid/semi-arid/semi-humid regions, potentially leading to a cooling effect. However, in humid regions, albedo warming completely surpasses ET cooling, causing a pronounced warming. Our findings suggest that using appropriate strategies to protect vulnerable dryland ecosystems from degradation, should lead to future benefits related to greening ecosystems and mitigation for rising temperatures.