bluuebunny/crossref_metadata_2025_split huggingface dataset:
Newspaper depictions of mental and physical health
Authors: Chen Marian , Lawrie Stephen 
Published: December 2017

Aims and methodMedia portrayals of mental illness have long been recognised as being misleading and stigmatising. Following the campaigns of several advocacy groups to address this issue, we aimed to evaluate the impact on mental health reporting over time. We repeated a survey we did 15 years ago using the same methods. Nine UK daily newspapers were surveyed over a 4-week period and coded with a schema to analyse the reporting of mental health compared with physical health.ResultsIn total, 963 articles – 200 on mental health and 763 on physical health – were identified. Over half of the articles on mental health were negative in tone: 18.5% indicated an association with violence compared with 0.3% of articles on physical health. However, there were more quotes from patients with mental disorders than physical disorders (22.5% v. 19.7%) and an equal mention of treatment and rehabilitation.Clinical implicationsMental health in print media remains tainted by themes of violence, however some improvement in reporting in recent years is evident, in particular by providing a voice for people with mental illness.
Population-Based Study to Determine the Health System Costs of Using the 21-Gene Assay
Authors: Mittmann Nicole , Earle Craig C. , Cheng Stephanie Y. , Julian Jim A. , Rahman Farah , Seung Soo Jin , Levine Mark N. 
Published: December 2017

Purpose The 21-gene assay Oncotype Dx (Genomic Health, Redwood City, CA) test is used to aid the decision about chemotherapy in patients with hormone receptor–positive breast cancer who received endocrine therapy. Economic studies to support test adoption used decision-analytic models with assumptions and data derived from disparate sources. The objective was to evaluate whether the 21-gene assay test resulted in an overall cost expense or saving to the health system. Patients and Methods One thousand participants enrolled in a field evaluation study, were linked to population-level health system administrative databases, and were observed for 20 months. The cost for the cohort, which included the cost of the test, subsequent treatments received, and health care encounters, was determined. The cost in the absence of the test was compared with the pretest recommendation about chemotherapy from the field study for a base case and under scenarios that reflected different adjuvant chemotherapy use. Overall health system costs and incremental costs were calculated. Results The 21-gene assay resulted in a net decrease in chemotherapy use of 23%. For the base case incremental analysis, the actual overall health system cost of this cohort, including the cost of 21-gene assay, was $29.2 million compared with $26.2 million in the absence of the test—an increase of $3.1 million. For three of the four scenario analyses, the actual overall cost to the health system exceeded the estimated cost in the absence of the test. Results showed that, when at least half of the population received adjuvant chemotherapy, the cost increased to $30.2 million. Conclusion The use of real-world administrative data showed that, despite lower rates of chemotherapy use, the 21-gene assay test results in an overall incremental cost to the health care system in the short-term under most assumptions.
Is Compliance With Surgical Care Improvement Project Cardiac (SCIP-Card-2) Measures for Perioperative β-Blockers Associated With Reduced Incidence of Mortality and Cardiovascular-Related Critical Quality Indicators After Noncardiac Surgery?
Authors: Kertai Miklos D. , Cooter Mary , Pollard Richard J. , Buhrman William , Aronson Solomon , Mathew Joseph P. , Stafford-Smith Mark 
Published: December 2017

BACKGROUND:While continuation of β-blockers (BBs) perioperatively has become a national quality improvement measure, the relationship between BB withdrawal and mortality and cardiovascular-related critical quality indicators has not been studied in a contemporary cohort of patients undergoing noncardiac surgery.METHODS:For this retrospective study, the quality assurance database of a large community-based anesthesiology group practice was used to identify 410,288 surgical cases, 18 years of age or older, who underwent elective or emergent noncardiac surgical procedures between January 1, 2009, and December 31, 2014. Each surgical case that was withdrawn from BBs perioperatively was propensity matched by clinical and surgical characteristics to 4 cases that continued BBs perioperatively. Subsequently, multivariable conditional logistic regression analyses were performed in the matched cohort to determine the extent to which withdrawal of perioperative BBs was independently associated with mortality as the primary outcome and cardiovascular-related critical quality indicators as the secondary outcome (need for vasopressor, electrocardiographic changes requiring treatment, unplanned admission to intensive care unit, postanesthesia care unit stay >2 hours, and a combination of cardiac arrest and myocardial infarction) within 48 hours postoperatively.RESULTS:Of the 66,755 (16%) cases in the cohort admitted on BB therapy, BBs were withdrawn in 3829 (6%) and continued in 62,926 (94%). Propensity score matching resulted in an analysis cohort of 19,145 cases. Withdrawal of perioperative BBs in the multivariable conditional logistic regression analysis was significantly associated with an increased risk for mortality (odds ratio [OR], 3.61; 95% confidence interval [CI], 1.75–7.35;P= .0003), but a significantly decreased risk for need of blood pressure support requiring vasopressor initiation (OR, 0.84; 95% CI, 0.76–0.92;P= .0003) and extended postanesthesia care unit stay (OR, 0.69; 95% CI, 0.54–0.88;P= .004) within 48 hours after noncardiac surgery.CONCLUSIONS:Perioperative withdrawal of BBs was associated with increased risk for mortality within 48 hours after noncardiac surgery and with decreased risk for need of vasopressor during the early postoperative period and a shorter stay in the postanesthesia care unit.
Inflammasome activation and assembly at a glance
Authors: Malik Ankit , Kanneganti Thirumala-Devi 
Published: December 2017

ABSTRACT
Inflammasomes are multimeric protein complexes that typically comprise a sensor, an adaptor and the zymogen procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1 through proximity-induced self-cleavage, which further induces maturation of interleukins 1β and 18 (IL-1β and IL-18) through proteolytic cleavage of pro-IL-1β and pro-IL-18. Activated caspase-1 also cleaves gasdermin D, which leads to a particular form of cell death called pyroptosis. Mutations in genes that encode inflammasome components are associated with many inflammatory disorders, and studies in the past decade have highlighted the importance of appropriate activation of the inflammasome in homeostasis and disease pathogenesis. Therefore, much attention is being paid to uncover the modulators and regulators of inflammasome assembly and pyroptosis. This Cell Science at a Glance article and accompanying poster outlines the concepts in the activation of inflammasome sensors and assembly of the inflammasome platform. We also discuss recent insights into the mechanisms of regulation of inflammasome activity and the induction of cell death by pyroptosis.
Validation of a high-performance liquid chromatography method for thiopurine S-methyltransferase activity in whole blood using 6-mercaptopurine as substrate
Authors: Rieger Hannah , Schmidt Patrik , Schaeffeler Elke , Abe Manabu , Schiffhauer Mira , Schwab Matthias , von Ahsen Nicolas , Zurek Gabriela , Kirchherr Hartmut , Shipkova Maria , Wieland Eberhard 
Published: December 2017

AbstractBackground:Variation in metabolism, toxicity and therapeutic efficacy of thiopurine drugs is largely influenced by genetic polymorphisms in the thiopurine S-methyltransferase (TPMT) gene. Determination of TPMT activity is routinely performed in patients to adjust drug therapy.Methods:We further optimized a previously established high-performance liquid chromatography (HPLC) method by measuring TPMT activity in whole blood instead of isolated erythrocytes, which is based on conversion of 6-mercaptopurine to 6-methylmercaptopurine using S-adenosyl-methionine as methyl donor.Results:The simplified TPMT whole-blood method showed similar or better analytical and diagnostic performance compared with the former erythrocyte assay. The whole-blood method was linear for TPMT activities between 0 and 40 nmol/(mL·h) with a quantification limit of 0.1 nmol/(mL·h). Within-day imprecision and between-day imprecision were ≤5.1% and ≤8.5%, respectively. The optimized method determining TPMT activity in whole blood (y) showed agreement with the former method determining TPMT activity in erythrocytes (x) (n=45, y=1.218+0.882x; p>0.05). Phenotype-genotype concordance (n=300) of the whole-blood method was better when TPMT activity was expressed per volume of whole blood (specificity 92.2%), whereas correction for hematocrit resulted in lower genotype concordance (specificity 86.9%). A new cutoff for the whole-blood method to distinguish normal from reduced TPMT activity was determined at ≤6.7 nmol/(mL·h).Conclusions:This optimized TPMT phenotyping assay from whole blood using 6-MP as substrate is suitable for research and routine clinical analysis.
Effect of preanalytical and analytical variables on the clinical utility of mean platelet volume
Authors: Buttarello Mauro , Mezzapelle Giacomo , Plebani Mario 
Published: December 2017

AbstractBackground:The study endpoint was to analyze the effect of preanalytical (time, temperature, anticoagulant) and analytical (imprecision, correlation between volume and platelet concentration) variables on mean platelet volume (MPV). A further aim was to calculate in an adult population the reference intervals using the Sysmex XE-5000 analyzer. A critical evaluation was also made of the clinical utility of these parameters.Methods:Analyses of the above values were performed in duplicate in 170 healthy adults of both sexes: (1) within 30 min from collection, and (2) after 4 h. To evaluate stability over time, the value of the platelet parameters of 20 subjects were determined, a re-analysis being performed for a period of up to 24 h on samples maintained at room temperature and 4°C using either K2-EDTA or Na-citrate as anticoagulants.Results:The stability over time of MPV closely depends on the anticoagulant used, storage temperature and time interval between venipuncture and analysis. An inverse, non-linear correlation between MPV and platelet count was also found.Conclusions:In view of their effect on MPV and other related indices, the preanalytical and analytical variables make them, little more than experimental.
Peritoneal innervation: embryology and functional anatomy
Authors: Struller Florian , Weinreich Frank-Jürgen , Horvath Philipp , Kokkalis Marios-Konstantinos , Beckert Stefan , Königsrainer Alfred , Reymond Marc A. 
Published: December 2017

AbstractThe parietal peritoneum (PP) is innervated by somatic and visceral afferent nerves. PP receives sensitive branches from the lower intercostal nerves and from the upper lumbar nerves. Microscopically, a dense network of unmyelinated and myelinated nerve fibers can be found all over the PP. The unmyelinated fibers are thin and are ending just underneath the PP. The myelinated fibers can penetrate the PP to reach the peritoneal cavity, where they lose their myelin sheath and are exposed to somatic and nociceptive stimuli. PP is sensitive to pain, pressure, touch, friction, cutting and temperature. Noxious stimuli are perceived as a localized, sharp pain. The visceral peritoneum (VP) itself is not innervated, but the sub-mesothelial tissue is innervated by the autonomous nerve system. In contrast to the PP, the visceral submesothelium also receives fibers from the vagal nerve, in addition to the spinal nerves. VP responds primarily to traction and pressure; not to cutting, burning or electrostimulation. Painful stimuli of the VP are poorly localized and dull. Pain in a foregut structure (stomach, duodenum or biliary tract) is referred to the epigastric region, pain in a midgut structure (appendix, jejunum, or ileum) to the periumbilical area and pain from a hindgut source (distal colon or rectum) is referred to the lower abdomen or suprapubic region. Peritoneal adhesions can contain nerve endings. Neurotransmitters are acetylcholine, VIP, serotonin, NO, encephalins, CGRP and substance P. Chronic peritoneal pain can be exacerbated by neurogenic inflammation, e.g. by endometriosis.
Zum Stromkonsum von Haushalten in Grundsicherung: Eine empirische Analyse für Deutschland
Authors: Heindl Peter , Aigeltinger Gerd , Liessem Verena , Römer Daniel , Schwengers Clarita , Vogt Claire 
Published: December 2017

ZusammenfassungIn diesem Aufsatz untersuchen die Autoren Stromkonsum und Stromkosten von Haushalten in Grundsicherung in Deutschland. Dazu werten sie einen Datensatz aus, der mehr als 19.500 Haushalte mit Leistungsbezug nach SGB II und SGB XII in verschiedenen Haushaltszusammensetzungen umfasst. Die Ergebnisse der empirischen Analyse zeigen, dass die Haushaltszusammensetzung sowie die Art der Warmwasserbereitung erheblichen Einfluss auf die entstehenden Verbräuche und Kosten haben. Insbesondere die elektrische Warmwasserbereitung verursacht erhebliche zusätzliche Kosten. Es kann nur eine schwache Nachfragereaktion der Haushalte auf Veränderungen des Strompreises nachgewiesen werden. Insgesamt zeigt sich, dass die veranschlagten Anteile für Strom in den Regelbedarfen nach SGB II und SGB XII im Durchschnitt nicht zur Begleichung der Stromkosten ausreichen. Dieses Problem verschärft sich insbesondere in Fällen, in denen Warmwasser mit Strom bereitet wird.
Effect of crude oil and well stream chemical on glass fiber epoxy composite pipes
Authors: Al-Samhan Meshal , Al-Enezi Salah , Al-Banna Aseel , Yussuf Abdirahman 
Published: December 2017

Abstract
Glass-reinforced epoxy (GRE) pipes are used as an alternative to carbon steel pipes in desert oil field applications owing to their good properties such as chemical resistance, thermal resistance and mechanical properties. In the desert oil field service, the pipes are exposed to different harsh environmental conditions such as high temperature, humidity and ultraviolet radiation. The harsh environmental conditions can affect the properties of GRE pipes, which, in turn, can impact their performance. The present study covers the effects of environment factors such as crude oil and well stream chemicals on the properties of GRE pipes. The pipe samples were aged in wet crude, effluent water and scale inhibitor to simulate the exposure to desert oil field service. The aged samples were evaluated for their mechanical properties, thermal stability and chemical resistance. The compositional change in the composite structures was characterized using Fourier transform infrared spectroscopy and matrix deterioration by scanning electron microscopy. Swelling studies revealed no significant diffusion by the chemical media. Tensile testing showed a slight change in tensile strength with aging in crude oil. In addition, all the pipe samples showed adequate thermal stability to withstand harsh environmental conditions.
Wear analysis of the composite coating in a long sliding time by dissipated energy approach
Authors: Shao Jin Zhong , Li Jun , Qu Cui Cui , Song Rui , Bai Lv Lin , Chen Jia Li 
Published: December 2017

Abstract
A wear test of Ti2Ni/TiNi-based coating reinforced by TiC/TiB against a YG6X [94 wt.% tungsten carbide (WC) and 6 wt.% cobalt (Co)] ball in a sliding time of 712 h was carried out using a ball-on-disc reciprocating motion mode. The relationship between accumulated dissipated energy (∑E) and accumulated wear volume (∑V) was accurately established via the dissipated energy approach. Three wear stages were found: initial wear stage (0–200 min), breaking-in wear stage (200–14,520 min), and steady wear stage (14,520–42,720 min). At the initial wear stage, the relationship between ∑E and ∑V satisfied the equation ∑V=42.5288∑E+0.019. The curvilinear relationship in the other two stages was fitted into the equation ∑V=-0.0029∑E
4+0.064∑E3-0.5353∑E
2+2.0653∑E+0.9938. The wear mechanism in the entire wear process was revealed. ∑E at the initial wear stage was mainly used for debris formation. At the breaking-in wear stage, ∑E was dissipated by generating the friction heat, which led to the formation of a protective layer. At the steady wear stage, ∑E was used to generate friction heat as well as to damage and restore the protective layer. The detection results of the protective layer via energy dispersive spectroscopy showed that this layer was composed of oxides and WC debris.
Inhibition in Simple Cell Receptive Fields Is Broad and OFF-Subregion Biased
Authors: Taylor M. Morgan , Sedigh-Sarvestani Madineh , Vigeland Leif , Palmer Larry A. , Contreras Diego 
Published: December 2017

Inhibition in thalamorecipient layer 4 simple cells of primary visual cortex is believed to play important roles in establishing visual response properties and integrating visual inputs across their receptive fields (RFs). Simple cell RFs are characterized by nonoverlapping, spatially restricted subregions in which visual stimuli can either increase or decrease the firing rate of the cell, depending on contrast. Inhibition is believed to be triggered exclusively from visual stimulation of individual RF subregions. However, this view is at odds with the known anatomy of layer 4 interneurons in visual cortex and differs from recent findings in mouse visual cortex. Here we show within vivointracellular recordings in cats that while excitation is restricted to RF subregions, inhibition spans the width of simple cell RFs. Consequently, excitatory stimuli within a subregion concomitantly drive excitation and inhibition. Furthermore, we found that the distribution of inhibition across the RF is stronger toward OFF subregions. This inhibitory OFF-subregion bias has a functional consequence on spatial integration of inputs across the RF. A model based on the known anatomy of layer 4 demonstrates that the known proportion and connectivity of inhibitory neurons in layer 4 of primary visual cortex is sufficient to explain broad inhibition with an OFF-subregion bias while generating a variety of phase relations, including antiphase, between excitation and inhibition in response to drifting gratings.SIGNIFICANCE STATEMENTThe wiring of excitatory and inhibitory neurons in cortical circuits is key to determining the response properties in sensory cortex. In the visual cortex, the first cells that receive visual input are simple cells in layer 4. The underlying circuitry responsible for the response properties of simple cells is not yet known. In this study, we challenge a long-held view concerning the pattern of inhibitory input and provide results that agree with current known anatomy. We show here that inhibition is evoked broadly across the receptive fields of simple cells, and we identify a surprising bias in inhibition within the receptive field. Our findings represent a step toward a unified view of inhibition across different species and sensory systems.
Inactivation of NMDA Receptors in the Ventral Tegmental Area during Cocaine Self-Administration Prevents GluA1 Upregulation but with Paradoxical Increases in Cocaine-Seeking Behavior
Authors: Guzman Daniel , Carreira Maria B. , Friedman Allyson K. , Adachi Megumi , Neve Rachael L. , Monteggia Lisa M. , Han Ming-Hu , Cowan Christopher W. , Self David W. 
Published: December 2017

Cocaine self-administration increases expression of GluA1 subunits in ventral tegmental area (VTA) dopamine neurons, which subsequently enhance the motivation for cocaine. This increase in GluA1 may be dependent on concomitant NMDA receptor (NMDAR) activation during self-administration, similar to cocaine-induced long-term potentiation in the VTA. In this study, we used viral-mediated expression of a dominant-negative GluN1 subunit (HSV-dnGluN1) in VTA neurons to study the effect of transient NMDAR inactivation on the GluA1 increases induced by chronic cocaine self-administration in male rats. We found that dnGluN1 expression in the VTA limited to the 3 weeks of cocaine self-administration prevents the subsequent increase in tissue GluA1 levels when compared with control infusions of HSV-LacZ. Surprisingly, dnGluN1 expression led to an enhancement in the motivation to self-administer cocaine as measured using a progressive ratio reinforcement schedule and to enhanced cocaine seeking measured in extinction/reinstatement tests following an extended 3 week withdrawal period. Despite blocking tissue GluA1 increases in cocaine self-administering animals, the HSV-dnGluN1 treatment resulted in increased membrane levels of GluA1 and GluN2B, along with markedly higher locomotor responses to intra-VTA infusions of AMPA, suggesting a paradoxical increase in VTA AMPA receptor responsiveness. Together, these data suggest that NMDARs mediate cocaine-induced increases in VTA GluA1 expression, but such transient NMDAR inactivation also leads to compensatory scaling of synaptic AMPA receptors that enhance the motivational for cocaine.SIGNIFICANCE STATEMENTDopamine neurons in the ventral tegmental area (VTA) are critical substrates of drug rewards. Animal models indicate that chronic cocaine use enhances excitatory glutamatergic input to these neurons, making them more susceptible to environmental stimuli that trigger drug craving and relapse. We previously found that self-administration of cocaine increases AMPA glutamate receptors in the VTA, and this effect enhances motivation for cocaine. Here we report that the mechanism for this upregulation involves NMDA receptor activity during cocaine use. While interference with NMDA receptor function blocks AMPA receptor upregulation, it also produces a paradoxical enhancement in membrane AMPA receptor subunits, AMPA responsiveness, and the motivation for cocaine. Thus, pharmacotherapy targeting NMDA receptors may inadvertently produce substantial adverse consequences for cocaine addiction.