bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of the Precursor of Interleukin‐1β and Peritoneal Inflammation—Role in Pathogenesis of Endometriosis
Authors: Sikora Justyna , Mielczarek‐Palacz Aleksandra , Kondera‐Anasz Zdzisława 
Published: March 2016

BackgroundThe most important proinflammatory cytokine is interleukin (IL)‐1β, however its precursor, prointerleukin‐1β (proIL‐1β), can also potentiate inflammatory state. The aim of this study was to explore the involvement of proIL‐1β in pathogenesis of endometriosis. For this purpose, we evaluated concentrations of proIL‐1β, IL‐1β, and soluble IL‐1 receptor type 2 (sIL‐1R2) in peritoneal fluid (PF) and macrophage culture medium of women with endometriosis.MethodsPF from 55 women with and without endometriosis was collected during laparoscopy. Peritoneal macrophages were cultured in basal and stimulated with lipopolysaccharide (LPS) conditions. Concentrations of cytokines were measured with enzyme‐linked immunosorbent assays (ELISA).ResultsPF proIL‐1β and IL‐1β levels in endometriosis women were higher than in the control. Higher basal and stimulated macrophage secretion of cytokines in endometriosis patients than in the control was observed. However, in endometriosis, there was a higher level of proIL‐1β than for the mature molecule. Additionally, lower PF and macrophages culture medium sIL‐1R2 levels were observed in women with endometriosis.ConclusionsAbnormal proIL‐1β concentration in PF and higher macrophage secretion can escalate peritoneal inflammation and endometriosis formation. The results are presented as a total IL‐1β, which is a sum of proIL‐1β and IL‐1β, and we believe that it reflects the actual cytokine production. The imbalance among all studied cytokines in endometriosis may be linked with an ability to transform acute inflammation to the chronic inflammation.
Positive Psychology and Disaster Mental Health: Strategies for Working with Children and Adolescents
Authors: Vernberg Eric M. , Hambrick Erin P. , Cho Bridget , Hendrickson Michelle L. 
Published: March 2016

Positive psychology concepts and principles can be incorporated into preparedness, crisis response, and recovery phases of disaster mental health efforts to address the needs of children, adolescents, and families. This article articulates general developmental considerations for applying positive psychology in disaster mental health contexts and discusses how 5 essential elements of immediate and midterm mass trauma intervention identified by Hobfoll et al. (2007) may be infused in applications of positive psychology for children and adolescents. Specific strategies for working with children, adolescents, and their families in home, community, and school contexts are drawn in part from disaster mental health resources developed jointly by the National Child Traumatic Stress Network and National Center for Posttraumatic Stress Disorder, including the Psychological First Aid Field Operations Guide (Brymer et al., 2006), the Skills for Psychological Recovery Field Operations Guide (Berkowitz et al., 2010), and the Psychological First Aid for Schools Field Operations Manual (Brymer et al., 2012). Two case examples illustrate the use of positive psychology principles.
Role of quantitative intravoxel incoherent motion parameters in the preoperative diagnosis of nodal metastasis in patients with rectal carcinoma
Authors: Qiu Lin , Liu Xiao‐Ling , Liu Si‐Run , Weng Ze‐Ping , Chen Xiao‐Qiao , Feng You‐Zhen , Cai Xiang‐Ran , Guo Chang‐Yu 
Published: March 2016

PurposeTo evaluate the diagnostic value of intravoxel incoherent motion imaging (IVIM) in differentiating metastatic and nonmetastatic lymph nodes in patients with rectal carcinoma.Materials and MethodsIn all, 68 patients with histologically proven rectal carcinoma underwent an IVIM sequence (b = 0, 25, 50, 75, 100, 150, 200, 400, 600, 800, 1000, 1200, 1500, and 2000 s/mm2) on a 3.0T MRI scanner. The IVIM parameters (D, D*, f, and apparent diffusion coefficient [ADC] values) in metastatic and nonmetastatic lymph nodes were measured and calculated. Receiver‐operating characteristic (ROC) analyses were conducted to determine the optimal thresholds, the sensitivities, and specificities for differentiation.ResultsMean D, f, and ADC values of metastatic lymph nodes were significantly greater than those of the normal lymph nodes (P < 0.01), whereas the mean D* value of metastatic lymph node was statistically lower (P = 0.03). The AUC, sensitivity, specificity, and the cutoff value, respectively, for differentiating metastatic from nonmetastatic lymph nodes for D, D*, f, and ADC were as follows: D, 0.9460, 89.25%, 91.04%, and 1.14 × 10−3mm2/s; D*, 0.6930, 64.18%, 82.80%, and 7.02 × 10−3mm2/s; f, 0.7810, 92.47%, 55.22%, and 0.27%; ADC, 0.8970, 87.10%, 88.06%, and 0.80 × 10−3mm2/s. The ROC curves demonstrated that the area under the ROC (AUC) of the D, ADC, f, and D* values successively decreased, and D had the highest AUC, with D* values being lowest.ConclusionAn IVIM sequence may be helpful in diagnosing metastatic lymph nodes of rectal carcinoma. Average D and ADC values are more sensitive than f and D* values in this differentiation. J. MAGN. RESON. IMAGING 2016;44:1031–1039.
Whole‐body vibration induces pain and lumbar spinal inflammation responses in the rat that vary with the vibration profile
Authors: Zeeman Martha E. , Kartha Sonia , Winkelstein Beth A. 
Published: March 2016

ABSTRACTWhole‐body vibration (WBV) is linked epidemiologically to neck and back pain in humans, and to forepaw mechanical allodynia and cervical neuroinflammation in a rodent model of WBV, but the response of the low back and lumbar spine to WBV is unknown. A rat model of WBV was used to determine the effect of different WBV exposures on hind paw behavioral sensitivity and neuroinflammation in the lumbar spinal cord. Rats were exposed to 30 min of WBV at either 8 or 15 Hz on days 0 and 7, with the lumbar spinal cord assayed using immunohistochemistry at day 14. Behavioral sensitivity was measured using mechanical stimulation of the hind paws to determine the onset, persistence, and/or recovery of allodynia. Both WBV exposures induce mechanical allodynia 1 day following WBV, but only the 8 Hz WBV induces a sustained decrease in the withdrawal threshold through day 14. Similarly, increased activation of microglia, macrophages, and astrocytes in the superficial dorsal horn of the lumbar spinal cord is only evident after the painful 8 Hz WBV. Moreover, extracellular signal‐regulated kinase (ERK)‐phosphorylation is most robust in neurons and astrocytes of the dorsal horn, with the most ERK phosphorylation occurring in the 8 Hz group. These findings indicate that a WBV exposure that induces persistent pain also induces a host of neuroimmune cellular activation responses that are also sustained. This work indicates there is an injury‐dependent response that is based on the vibration parameters, providing a potentially useful platform for studying mechanisms of painful spinal injuries. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1439–1446, 2016.
Establishment and characterization of a novel osteosarcoma cell line: CHOS
Authors: Liu Yunlu , Feng Xiaobo , Zhang Yukun , Jiang Hongyan , Cai Xianyi , Yan Xinxin , Huang Zengfa , Mo Fengbo , Yang Wen , Yang Cao , Yang Shuhua , Liu Xianzhe 
Published: March 2016

ABSTRACTOsteosarcoma has a well‐recognized bimodal distribution, with the first peak in adolescence and another in the elderly age‐group. The elderly patients have different clinical features and a poorer prognosis as compared to adolescents. To better understand the biological features of osteosarcoma in the elderly population, we established a new human osteosarcoma cell line from a 58‐year‐old man with primary chondroblastic osteosarcoma. After 6 months of continuous culture in vitro for over 50 passages, an immortalized cell line CHOS was established. The cell line was well‐characterized by cytogenetic, biomarker, functional, and histological analyses. The CHOS cells exhibited a spindle‐shaped morphology and a doubling time of 36 h. Cytogenetic analysis of CHOS cells revealed the loss of chromosome Y and the gain of chromosome 12. Quantitative real‐time polymerase chain reaction (RT‐PCR), Western blotting and/or immunofluorescence revealed the expression of chondroblastic, mesenchymal and tumor metastasis markers in the CHOS cells. Compared with the osteosarcoma cell line, the CHOS cells were found to be more sensitive to cisplatin and doxorubicin, but were resistant to methotrexate. The cell line was highly tumorigenic and maintained the histological characteristics and invasive nature of the original tumor. Furthermore, on immunohistochemical analysis, the xenografts and metastases were found to co‐express collagen II, aggrecan, vimentin and S100A4 that resembled the original tumor cells. Our results indicate, the potential of CHOS cell line to serve as a useful tool for further studies on the molecular biology of osteosarcoma, especially in the elderly patients. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2116–2125, 2016.
Collagen V expression is crucial in regional development of the supraspinatus tendon
Authors: Connizzo Brianne K. , Adams Sheila M. , Adams Thomas H. , Birk David E. , Soslowsky Louis J. 
Published: March 2016

ABSTRACTManipulations in cell culture and mouse models have demonstrated that reduction of collagen V results in altered fibril structure and matrix assembly. A tissue‐dependent role for collagen V in determining mechanical function was recently established, but its role in determining regional properties has not been addressed. The objective of this study was to define the role(s) of collagen V expression in establishing the site‐specific properties of the supraspinatus tendon. The insertion and midsubstance of tendons from wild type, heterozygous and tendon/ligament‐specific null mice were assessed for crimp morphology, fibril morphology, cell morphology, as well as total collagen and pyridinoline cross‐link (PYD) content. Fibril morphology was altered at the midsubstance of both groups with larger, but fewer, fibrils and no change in cell morphology or collagen compared to the wild type controls. In contrast, a significant disruption of fibril assembly was observed at the insertion site of the null group with the presence of structurally aberrant fibrils. Alterations were also present in cell density and PYD content. Altogether, these results demonstrate that collagen V plays a crucial role in determining region‐specific differences in mouse supraspinatus tendon structure. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2154–2161, 2016.
Detection of aflatoxin and surface mould contaminated figs by using Fourier transform near‐infrared reflectance spectroscopy
Authors: Durmuş Efkan , Güneş Ali , Kalkan Habil 
Published: March 2016

AbstractBACKGROUNDAflatoxins are toxic metabolites that are mainly produced by members of the Aspergillus section Flavi on many agricultural products. Certain agricultural products such as figs are known to be high risk products for aflatoxin contamination. Aflatoxin contaminated figs may show a bright greenish yellow fluorescence (BGYF) under ultraviolet (UV) light at a wavelength of 365 nm. Traditionally, BGYF positive figs are manually selected by workers. However, manual selection depends on the expertise level of the workers and it may cause them skin‐related health problems due to UV radiation.RESULTSIn this study, we propose a non‐invasive approach to detect aflatoxin and surface mould contaminated figs by using Fourier transform near‐infrared (FT‐NIR) reflectance spectroscopy. A classification accuracy of 100% is achieved for classifying the figs into aflatoxin contaminated/uncontaminated and surface mould contaminated/uncontaminated categories. In addition, a strong correlation has been found between aflatoxin and surface mould.CONCLUSIONCombined with pattern classification methods, the NIR spectroscopy can be used to detect aflatoxin contaminated figs non‐invasively. Furthermore, a positive correlation between surface mould and aflatoxin contamination leads to a promising alternative indicator for the detection of aflatoxin‐contaminated figs. © 2016 Society of Chemical Industry
Forest thinning changes movement patterns and habitat use by Pacific marten
Authors: Moriarty Katie M. , Epps Clinton W. , Zielinski William J. 
Published: March 2016

ABSTRACTSimplifying stand structure to reduce fuel density is a high priority for forest managers; however, affects to Pacific marten (Martes caurina) movement and connectivity are unknown. We evaluated whether thinning forests to reduce fuels influenced movements of Pacific marten. We collected movement paths from 22 martens using global positioning system telemetry to evaluate habitat selection and describe movement patterns. We quantified motion variance, speed, and path sinuosity in 3 stand types that differed in structural complexity (i.e., complex [dense], simple [thinned], and open). We hypothesized marten movement would differ between stand types and predicted that 1) martens would select stand types with increased structural complexity (complex > simple > open); 2) movements would increase in complexity (sinuosity, motion variance) and decrease in speed when martens traveled through stands with increased structural complexity; 3) speeds would increase during summer, indicating increased movement during the breeding season; and 4) males would move more rapidly because of their larger home ranges. Martens traveled 0.5–27.2 km/day and an average (SD) of 1.4 (0.4) km/hour. Martens selected home ranges with fewer openings compared to the study area overall. Within home ranges, martens strongly selected complex stands over simple stands and openings. Speed and movement complexity were most consistent over time and movements were more sinuous and slower in complex stand types compared with openings and simple stands. Movement was erratic and more linear in openings than in both complex and simple stands. In simple stands, movement patterns were intermediate between complex stands and openings. Females generally moved more slowly, sinuously, and less variably compared to males. Martens moved more quickly, less sinuously, and more variably during winter compared to summer. However, martens avoided stands with simplified structure, and the altered patterns of movement we observed in those stands suggested that such treatments may negatively affect the ability of martens to forage without increased risk of predation. Fuel treatments that simplify stand structure negatively affected marten movements and habitat connectivity. Given these risks, and because treating fuels is less justified in high elevation forests, the risks can be minimized by applying treatments below the elevations where martens typically occur. © 2016 The Wildlife Society.
Gartanin, an isoprenylated xanthone from the mangosteen fruit (Garcinia mangostana), is an androgen receptor degradation enhancer
Authors: Li Gongbo , Petiwala Sakina M. , Yan Miao , Won Jong Hoon , Petukhov Pavel A. , Johnson Jeremy J. 
Published: March 2016

ScopeAndrogen receptor (AR) has been a target of prostate cancer for nearly seven decades. In the last several years there has been an interest in identifying compounds that promote degradation of the androgen receptor. In the present study, gartanin, an isoprentylated xanthone in the mangosteen fruit, was evaluated for enhancing AR degradation, and inducing the unfolded protein response pathway.Methods and resultsThe interaction of gartanin with the ligand‐binding domain was characterized using a fluorescence polarization cell‐free assay and cell‐based FRET assay. Western blot analysis identified modulation of ER stress markers (BiP, PERK, IRE1, and CHOP) along with androgen receptor degradation. A computation simulation was performed to identify possible orientations of gartanin with the ligand‐binding domain. Utilizing a cell‐free and cell‐based FRET assays gartanin was found to interact with the ligand‐binding domain through a solely antagonist interaction. Interestingly, inhibition of CHOP, a critical component of the ER stress pathway, was observed to stabilize AR.ConclusionsGartanin is an isoprenylated xanthone that promotes AR degradation with evidence suggesting this process is critically regulated by the unfolded protein response pathway.
Hybrid radial‐cones trajectory for accelerated MRI
Authors: Johnson Kevin M. 
Published: March 2016

PurposeTo design and develop a series of ultrashort echo time k‐space sampling schemes, termed radial‐cones, which enables high sampling efficiency while maintaining compatibility with parallel imaging and compressed sensing reconstructions.Theory and MethodsRadial‐cones is a trajectory that samples three‐dimensional (3D) k‐space using a single base cone distributed along radial dimensions through a cost function–based optimization. Trajectories were generated for highly undersampled, short readout sampling and compared with 3D radial sampling in point spread function (PSF) analysis, digital and physical phantoms, and initial human volunteers. Parallel imaging reconstructions were evaluated with and without the use of compressed sensing–based regularization.ResultsCompared with 3D radial sampling, radial‐cones reduced the peak value and energy of PSF aliasing. In both digital and physical phantoms, this improved sampling behavior corresponded to a reduction in the root mean square error with a further reduction using compressed sensing. A slight increase in noise and a corresponding increase in apparent resolution was observed with radial‐cones. In in vivo feasibility testing, radial‐cones reconstructed images have a markedly lower number of apparent artifacts. Ultimate gains in imaging performance were limited by off‐resonance blurring.ConclusionRadial‐cones is an efficient non‐Cartesian sampling scheme enabling short echo readout with a high level of compatibility with parallel imaging and compressed sensing. Magn Reson Med 77:1068–1081, 2017. © 2016 International Society for Magnetic Resonance in Medicine
Pregnancy outcomes in prenatally diagnosed 47, XXX and 47, XYY syndromes: a 30‐year French, retrospective, multicentre study
Authors: Gruchy Nicolas , Blondeel Eleonore , Le Meur Nathalie , Joly‐Hélas Géraldine , Chambon Pascal , Till Marianne , Herbaux Martine , Vigouroux‐Castera Adeline , Coussement Aurélie , Lespinasse James , Amblard Florence , Jimenez Pocquet Mélanie , Lebel‐Roy Camille , Carré‐Pigeon Frédérique , Flori Elisabeth , Mugneret Francine , Jaillard Sylvie , Yardin Catherine , Harbuz Radu , Collonge‐Rame Marie‐Agnès , Vago Philippe , Valduga Mylène , Leporrier Nathalie , Vialard François 
Published: March 2016

AbstractObjectiveSex chromosome aneuploidies are frequently detected fortuitously in a prenatal diagnosis. Most cases of 47, XXX and 47, XYY syndromes are diagnosed in this context, and parents are thus faced with an unexpected situation. The objective of the present study was to characterize a French cohort of prenatally diagnosed cases of 47, XXX and 47, XYY and to evaluate the termination of pregnancy (TOP) rate before and after France's implementation of multidisciplinary centres for prenatal diagnosis in 1997.MethodsThis retrospective study identified respectively 291 and 175 cases of prenatally diagnosed 47, XXX and 47, XYY between 1976 and 2012. For each case, the indication, maternal age, karyotype and outcome were recorded.ResultsMost diagnoses of the two conditions were fortuitous. The occurrence of 47, XXX was associated with advanced maternal age. The overall TOP rate was higher for 47, XXX (22.9%) than for 47, XYY (14.6%), although this difference was not statistically significant. However, the TOP rates fell significantly after 1997 (from 41.1% to 11.8% for 47, XXX and from 25.8% to 6.7% for 47, XYY).ConclusionThe TOP rates after prenatal diagnoses of 47, XXX and 47, XYY fell significantly after 1997, following France's implementation of multidisciplinary centres for prenatal diagnosis. © 2016 John Wiley & Sons, Ltd.
Polysaccharide polyelectrolyte multilayer coating on poly(ethylene terephthalate)
Authors: Pérez‐Álvarez Leyre , Lizundia Erlantz , del Hoyo Sara , Sagasti Ariane , Rubio Leire Ruiz , Vilas J Luis 
Published: March 2016

AbstractSurface modification of poly(ethylene terephthalate) (PET) film was conducted by surface hydrolysis with NaOH and layer‐by‐layer assembly based on the electrostatic adsorption of oppositely charged polysaccharides: chitosan and hyaluronic acid. Hydrolysis of PET was verified by water contact angle variation of obtained surfaces and quantified using the toluidine blue O colorimetric method. Polysaccharide coatings of ca 200 nm could be observed using transmission electron microscopy for 10 chitosan/hyaluronic acid layers on PET film. Layer growth and degradation were easily monitored using UV‐visible spectroscopy after labelling of chitosan with fluorescein isothiocyanate of (FITC). Evaluation of degradation of the multilayers by FITC–chitosan release in phosphate buffer solution at pH = 7.4 showed that multilayers remain stable in phosphate buffer during the first 14 days after the loss of weakly bound polyelectrolytes. This fact makes the prepared polyelectrolyte multilayers interesting candidates to be employed as potential antibacterial implants and indwelling biomedical devices. © 2016 Society of Chemical Industry
Synthesis of organic‐inorganic hybrid gels by means of thiol‐ene and azide‐alkene reactions
Authors: Naga Naofumi , Nagino Hitomi , Furukawa Hidemitsu 
Published: March 2016

ABSTRACTOrganic–inorganic hybrid gels have been synthesized from a multi‐vinyl functional cyclic siloxane, 1,3,5,7‐tetravinyltetramethylcyclotetrasiloxane (TVMCTS), or a cubic silsesquioxane, octavinyloctasilasesquioxane (PVOSS), and α,ω‐dithiol compounds, 1,6‐hexanedithiol (HDT), 1,10‐decanedithiol (DDT), using thiol‐ene reaction in toluene. The network structure of the resulting gels, mesh size and mesh size distribution, was quantitatively characterized by means of a scanning microscopic light scattering (SMILS). The gels obtained from TVMCTS‐HDT formed homogeneous network structure with 1.5–1.6 nm mesh. Relaxation peaks derived from large clusters and/or micro gels were detected in the SMILS analysis of the TVMCTS‐DDT, PVOSS‐HDT, and PVOSS‐DDT gels besides those from the small meshes. The organic–inorganic hybrid gels were also synthesized from TVMCTS, PVOSS with α,ω‐diazide compounds, 1,6‐hexanediazide (HDA), 1,10‐decanediazide (DDA), using azide‐alkene reaction in toluene. All the gels obtained with the azide‐alkene reaction formed the homogeneous network structure. Enthalpy relaxation at the glass transition of the dried samples was detected by differential scanning calorimetry to study the network uniformity of the original gels. The gels synthesized by the azide‐alkene reaction showed larger enthalpy than the gels synthesized by the thiol‐ene reaction, indicating homogeneous network structure in the former gels. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2229–2238