bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging behind occluded areas with an iatrogenic perforated balloon: A safe, practical, and simple new method of visualizing the distal lumen in total occlusion
Authors: Balaban Yakup , Bektaş Osman , Bayramoğlu Adil , Gümrükçüoğlu Hasan A. , Kayışoğlu Ali H. 
Published: October 2017

ObjectiveIn the present study, we investigated the effectiveness and reliability of a new method that reveals whether guidewire advanced distal to the lesion is in the lumen in patients with acute (ATO) or chronic coronary total occlusion (CTO).MethodsForty‐one patients with symptomatic ATO and 22 patients with CTO who were admitted into our catheterization laboratory between January 2016 and March 2017 were included. In patients in whom antegrade filling could not be demonstrated after passing 0.014″ guidewire beyond the total lesion, a 1.25 × 15 mm balloon was punctured with a needle outside the operative field to visualize the total lesion. This perforated balloon was then used to deliver an opaque substance through this hole to visualize the distal part.ResultsThe mean age of our 63 patients was 66 ± 12 years. They had diabetes (57%), hypertension (100%), and a history of PCI (85%). The mean procedural time was 27 ± 6.8 min, and the mean volume of contrast material used was 93.9 ± 24 mL. This technique was 100% successful in accurately demonstrating the distal lumen and preventing complications.ConclusionThis new method we developed is much simpler and more useful than other methods for visualization of the true lumen because we can re‐orient the balloon at the time of opaque injection and use 190 cm guidewire without additional costs. Additionally, one balloon is sufficient for the operation.
Effect of sex difference in clinical presentation (stable coronary artery disease vs unstable angina pectoris or non‐ST‐elevation myocardial infarction vs ST‐elevation myocardial infarction) on 2‐year outcomes in patients undergoing percutaneous coronary intervention
Authors: Tang Xiao‐Fang , Song Ying , Xu Jing‐Jing , Ma Yuan‐Liang , Zhang Jia‐Hui , Yao Yi , He Chen , Wang Huan‐Huan , Jiang Ping , Jiang Lin , Liu Ru , Gao Zhan , Zhao Xue‐Yan , Qiao Shu‐Bin , Xu Bo , Yang Yue‐Jin , Gao Run‐Lin , Yuan Jin‐Qing 
Published: October 2017

ObjectiveTo determine whether there is a difference in 2‐year prognosis among patients across the spectrum of coronary artery disease undergoing percutaneous coronary intervention (PCI).MethodsWe analyzed all consecutive patients undergoing PCI at a single center from 1/1‐12/31/2013. Clinical presentations were compared between sexes according to baseline clinical, angiographic, and procedural characteristics and 2‐year (mean 730 ± 30‐day) outcomes.ResultsWe grouped 10 724 consecutive patients based on sex and clinical presentation. Among patients with ST‐elevation myocardial infarction (STEMI), rates of all‐cause death (6.7% vs 1.4%) and cardiac death (3.8% vs 1.1%) were significantly higher in women than in men (P < 0.05), but these rates did not differ between men and women with stable coronary artery disease (SCAD) and non‐ST‐elevation acute coronary syndrome ((NSTE‐ACS). Incidence of major bleeding was greater than in men only in those women presenting with ACS. After multivariable adjustment, female sex was not an independent predictor of outcomes in STEMI (hazard ratio [HR] for all‐cause death: 1.33, 95% confidence interval [CI]:0.52‐3.38; P = 0.55; HR for cardiac death: 0.69, 95%CI: 0.23‐2.09, P = 0.51], but was still an independent predictor of bleeding in STEMI (HR: 3.53, 95%CI: 1.26‐9.91, P = 0.017).ConclusionAmong STEMI patients, women had worse 2‐year mortality after PCI therapy, but female sex was not an independent predictor of mortality after adjustment for baseline characteristics. In STEMI patients, women were at higher bleeding risk than men after PCI, even after multivariable adjustment.
Functional analysis of ESM1 by siRNA knockdown in primary and metastatic head and neck cancer cells
Authors: Bender Onur , Gunduz Mehmet , Cigdem Sadik , Hatipoglu Omer Faruk , Acar Muradiye , Kaya Mesut , Grenman Reidar , Gunduz Esra , Ugur Kadriye Serife 
Published: October 2017

BackgroundGenetic factors play a large role in cancer, and thus, there is a great desire to understand the effects of different genes in cancer and to also develop gene therapy for better treatments. Therefore, the development of alternative diagnosis and therapy modalities is of utmost importance. The aim of our study was to illuminate the role of ESM1 (endothelial cell‐specific molecule‐1, also known as Endocan) in proliferation and migration of head and neck cancer, thus helping to pave the way for new treatment modalities and predictive biomarkers.MethodsESM1 expression was shown with immunofluorescence assay using confocal laser scanning microscope in primary and metastatic head and neck cancer cells. ESM1 expression was knocked down by RNA interference in head and neck cancer cells. Knockdown efficiency was evaluated by quantitative real‐time RT‐PCR and Western blot. Cell proliferation and migration assays were performed by xCELLigence real‐time cell analysis system.ResultsImmunofluorescence assay showed nuclear localization and high expression of ESM1 in primary and metastatic head and neck cancer cells. ESM1 mRNA and protein levels were significantly decreased in ESM1‐knockdown cells compared to control. ESM1‐knockdown cells showed reduced proliferation and migration activity when compared to control cells.ConclusionThese findings suggest that ESM1 has roles on proliferation and migration of head and neck cancer cells.
Dynamin‐related protein 1‐mediated mitochondrial fission contributes to post‐traumatic cardiac dysfunction in rats and the protective effect of melatonin
Authors: Ding Mingge , Ning Jiao , Feng Na , Li Zeyang , Liu Zhenhua , Wang Yuanbo , Wang Yueming , Li Xing , Huo Cong , Jia Xin , Xu Rong , Fu Feng , Wang Xiaoming , Pei Jianming 
Published: October 2017

AbstractMechanical trauma (MT) causes myocardial injury and cardiac dysfunction. However, the underlying mechanism remains largely unclear. This study investigated the role of mitochondrial dynamics in post‐traumatic cardiac dysfunction and the protective effects of melatonin. Adult male Sprague Dawley rats were subjected to 5‐minute rotations (200 revolutions at a rate of 40 rpm) to induce MT model. Melatonin was administrated intraperitoneally 5 minute after MT. Mitochondrial morphology, myocardial injury, and cardiac function were determined in vivo. There was smaller size of mitochondria and increased number of mitochondria per μm2 in the hearts after MT when the secondary myocardial injury was induced. Melatonin treatment at the dose of 30 mg/kg reduced serine 616 phosphorylation of Drp1 and inhibited mitochondrial Drp1 translocation and mitochondrial fission in the hearts of rats subjected to MT, which contributed to the reduction of myocardial injury and the improvement of cardiac function. In vitro, H9c2 cells cultured in 20% traumatic plasma (TP) for 12 hour showed enhanced mitochondrial fission, mitochondrial membrane potential (∆Ψm) loss, mitochondrial cytochrome c release, and decreased mitochondrial complex I‐IV activities. Pretreatment with melatonin (100 μmol/L) efficiently inhibited TP‐induced mitochondrial fission, ∆Ψm loss, cytochrome c release, and improved mitochondrial function. Melatonin's protective effects were attributed to its role in suppressing plasma TNF‐α overproduction, which was responsible for Drp1‐mediated mitochondrial fission. Taken together, our results demonstrate for the first time that abnormal mitochondrial dynamics is involved in post‐traumatic cardiac dysfunction. Melatonin has significant pharmacological potential in protecting against MT‐induced cardiac dysfunction by preventing excessive mitochondrial fission.
Foetal development of skeletal muscle in bovines as a function of maternal nutrition, foetal sex and gestational age
Authors: Gionbelli T. R. S. , Veloso C. M. , Rotta P. P. , Valadares Filho S. C. , C. Carvalho B. , Marcondes M. I. , S. Cunha C. , Novaes M. A. S. , Prezotto L. D. , Duarte M. S. , Gionbelli M. P. 
Published: October 2017

SummaryTo determine the effects of maternal nutrition on modifications of foetal development of the skeletal muscle and possible increase in the potential of skeletal muscle growth in cattle, gestating cows were either fed 190% NRC recommendations (overnourished; ON) or 100% NRC recommendation (control; CO). Interaction between maternal nutrition (MN) and the foetal sex (FS) was also investigated. Foetuses were necropsied at four different time points throughout gestation (139, 199, 241 and 268 days of gestation) to assess the mRNA expression of myogenic, adipogenic and fibrogenic markers in skeletal muscle. Phenotypic indicators of the development of skeletal muscle fibres, intramuscular lipogenesis and collagen development were also evaluated. Modifications in mRNA expression of skeletal muscle of foetuses were observed in function of MN and FS despite the lack of effect of MN and FS on foetal weight at necropsy. Maternal ON increased the mRNA expression of the myogenic marker Cadherin‐associated protein, beta 1 (CTNNB1) and adipogenic markers Peroxissome proliferator‐activated receptor gamma (PPARG) and Zinc finger protein 423 (ZNF423) at midgestation. However, no differences on foetal skeletal muscle development were observed between treatments at late gestation indicating that a compensatory development may have occurred on CO foetuses making the effect of MN on skeletal muscle development not significant at late gestation. Moreover, our data have shown an evidence of sexual dimorphism during foetal stage with a greater skeletal muscle development in male than in female foetuses. In conclusion, providing a higher nutritional level to pregnant cows changes the trajectory of the development of skeletal muscle during midgestation, but apparently does not change the potential of post‐natal growth of muscle mass of the offspring, as no differences in skeletal muscle development were observed in late gestation.
Effect of Aging on Processes of Motor Inhibition
Authors: Tsuchida Noriaki , Kawakami Miki 
Published: October 2017

AbstractThe processes of inhibiting uninstructed motor responses were examined to identify age differences. Younger adults (n = 40) and older adults (n = 38) participated in two experiments using the Stimulus‐Response Compatibility task. They were asked to push the switch on the side contralateral to the stimulus. The results indicated that responses induced by visual stimuli were gradually inhibited as a result of repeated trials on the contralateral (not‐induced) side, and no age differences were noted. The addition of a color‐discrimination task to the Stimulus‐Response Compatibility task inhibited impulsive response induced by visual stimuli in younger adults only. These results indicate that certain processes of inhibiting motor responses are affected by aging, whereas other processes remain unaffected.
Prevalence, predictors and correlates of insomnia in US army soldiers
Authors: Klingaman Elizabeth A. , Brownlow Janeese A. , Boland Elaine M. , Mosti Caterina , Gehrman Philip R. 
Published: October 2017

SummaryThe objective of this study was to investigate the rates, predictors and correlates of insomnia in a national sample of US Army soldiers. Data were gathered from the cross‐sectional survey responses of the All‐Army Study, of the Army Study to Assess Risk and Resilience in Service members. Participants were a representative sample of 21 499 US Army soldiers who responded to the All‐Army Study self‐administered questionnaire between 2011 and 2013. Insomnia was defined by selected DSM‐5 criteria using the Brief Insomnia Questionnaire. The results highlight significant functional difficulties associated with insomnia among US soldiers, as well as insights into predictors of insomnia specific to this population. Insomnia was present in 22.76% of the sample. Predictors of insomnia status in logistic regression included greater number of current mental health disorders, less perceived open lines of communication with leadership, less unit member support and less education. Insomnia had global, negative associations with health, social functioning, support, morale, work performance and Army career intentions. The results provide the strongest evidence to‐date that insomnia is common in a military population, and is associated with a wide array of negative factors in the domains of health, military readiness and intentions to remain in military careers.
Altered Serum Thyrotropin Concentrations in Dogs with Primary Hypoadrenocorticism before and during Treatment
Authors: Reusch C.E. , Fracassi F. , Sieber‐Ruckstuhl N.S. , Burkhardt W.A. , Hofer‐Inteeworn N. , Schuppisser C. , Stirn M. , Hofmann‐Lehmann R. , Boretti F.S. 
Published: October 2017

BackgroundThyrotropin (TSH) can be increased in humans with primary hypoadrenocorticism (HA) before glucocorticoid treatment. Increase in TSH is a typical finding of primary hypothyroidism and both diseases can occur concurrently (Schmidt's syndrome); therefore, care must be taken in assessing thyroid function in untreated human patients with HA.ObjectiveEvaluate whether alterations in cTSH can be observed in dogs with HA in absence of primary hypothyroidism.AnimalsThirty dogs with newly diagnosed HA, and 30 dogs in which HA was suspected but excluded based on a normal ACTH stimulation test (controls) were prospectively enrolled.MethodscTSH and T4 concentrations were determined in all dogs and at selected time points during treatment (prednisolone, fludrocortisone, or DOCP) in dogs with HA.ResultscTSH concentrations ranged from 0.01 to 2.6 ng/mL (median 0.29) and were increased in 11/30 dogs with HA; values in controls were all within the reference interval (range: 0.01–0.2 ng/dL; median 0.06). There was no difference in T4 between dogs with increased cTSH (T4 range 1.0‐2.1; median 1.3 μg/dL) compared to those with normal cTSH (T4 range 0.5‐3.4, median 1.4 μg/dL;P=0.69) and controls (T4 range 0.3‐3.8, median 1.8 μg/dL;P=0.35). After starting treatment, cTSH normalized after 2–4 weeks in 9 dogs and after 3 and 4 months in 2 without thyroxine supplementation.Conclusions and Clinical RelevanceEvaluation of thyroid function in untreated dogs with HA can lead to misdiagnosis of hypothyroidism; treatment with glucocorticoids for up to 4 months can be necessary to normalize cTSH.
Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum
Authors: Liang Yue , Xiong Wei , Steinkellner Siegrid , Feng Jie 
Published: October 2017

SummaryThe fungus Sclerotinia sclerotiorum is a necrotrophic plant pathogen causing significant damage on a broad range of crops. This fungus produces sclerotia that serve as the long‐term survival structures in the life cycle and the primary inoculum in the disease cycle. Melanin plays an important role in protecting mycelia and sclerotia from ultraviolet radiation and other adverse environmental conditions. In this study, two genes, SCD1 encoding a scytalone dehydratase and THR1 encoding a trihydroxynaphthalene reductase, were disrupted by target gene replacement, and their roles in mycelial growth, sclerotial development and fungal pathogenicity were investigated. Phylogenetic analyses indicated that the deduced amino acid sequences of SCD1 and THR1 were similar to the orthologues of Botrytis cinerea. Expression of SCD1 was at higher levels in sclerotia relative to mycelia. THR1 was expressed at similar levels in mycelia and sclerotia at early stages, but was up‐regulated in sclerotia at the maturation stage. Disruption of SCD1 or THR1 did not change the pathogenicity of the fungus, but resulted in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to ultraviolet light.
The first brain: Species comparisons and evolutionary implications for the enteric and central nervous systems
Authors: Furness J. B. , Stebbing M. J. 
Published: October 2017

AbstractBackgroundThe enteric nervous system (ENS) and the central nervous system (CNS) of mammals both contain integrative neural circuitry and similarities between them have led to the ENS being described as the brain in the gut.PurposeTo explore relationships between the ENS and CNS across the animal kingdom. We found that an ENS occurs in all animals investigated, including hydra, echinoderms and hemichordates that do not have a CNS. The general form of the ENS, which consists of plexuses of neurons intrinsic to the gut wall and an innervation that controls muscle movements, is similar in species as varied and as far apart as hydra, sea cucumbers, annelid worms, octopus and humans. Moreover, neurochemical similarities across phyla imply a common origin of the ENS. Investigation of extant species suggests that the ENS developed in animals that preceded the division that led to cnidaria (exemplified by hydra) and bilateria, which includes the vertebrates. The CNS is deduced to be a bilaterian development, later than the divergence from cnidaria. Consistent with the ENS having developed independent of the CNS, reciprocal connections between ENS and CNS occur in mammals, and separate neurons of ENS and CNS origin converge on visceral organs and prevertebral ganglia. We conclude that an ENS arose before and independently of the CNS. Thus the ENS can be regarded as the first brain.
Horses and the Embodiment of Elite Masculinity in the Dolenjska Hallstatt Culture
Authors: Frie Adrienne C. 
Published: October 2017

SummaryIn later prehistory horse ownership was a manifestation of wealth and physical prowess, and demonstrated access to distant lands. Because of the expense and restricted availability of horses, they are often reduced to indicators of status without more nuanced considerations of how lived human‐horse interactions enmeshed them in these status displays. To complicate the simple horse/status object equivalence, this article presents a specific case for the symbolic and social significance of horses in Early Iron Age south‐eastern Slovenia through the lens of equine iconography, and argues that horses and particularly equestrianism were essential to embodying elite masculine identity. Broadly, this article seeks to move beyond equating high‐status goods with high‐status people by discussing how particular events, bodily abilities and human‐animal relationships were all intertwined in the materialization of social distinction for a particular group.
High‐resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harbouring the Fusarium head blight resistance QTLQfhs.ifa‐5A
Authors: Buerstmayr Maria , Steiner Barbara , Wagner Christian , Schwarz Petra , Brugger Klaus , Barabaschi Delfina , Volante Andrea , Valè Giampiero , Cattivelli Luigi , Buerstmayr Hermann 
Published: October 2017

SummaryThe Qfhs.ifa‐5A allele, contributing to enhanced Fusarium head blight resistance in wheat, resides in a low‐recombinogenic region of chromosome 5A close to the centromere. A near‐isogenic RIL population segregating for the Qfhs.ifa‐5A resistance allele was developed and among 3650 lines as few as four recombined within the pericentromeric C‐5AS1‐0.40 bin, yielding only a single recombination point. Genetic mapping of the pericentromeric region using a recombination‐dependent approach was thus not successful. To facilitate fine‐mapping the physically large Qfhs.ifa‐5A interval, two gamma‐irradiated deletion panels were generated: (i) seeds of line NIL3 carrying the Qfhs.ifa‐5A resistance allele in an otherwise susceptible background were irradiated and plants thereof were selfed to obtain deletions in homozygous state and (ii) a radiation hybrid panel was produced using irradiated pollen of the wheat line Chinese Spring (CS) for pollinating the CS‐nullisomic5Atetrasomic5B. In total, 5157 radiation selfing and 276 radiation hybrid plants were screened for deletions on 5AS and plants containing deletions were analysed using 102 5AS‐specific markers. Combining genotypic information of both panels yielded an 817‐fold map improvement (cR/cM) for the centromeric bin and was 389‐fold increased across the Qfhs.ifa‐5A interval compared to the genetic map, with an average map resolution of 0.77 Mb/cR. We successfully proved that the RH mapping technique can effectively resolve marker order in low‐recombining regions, including pericentromeric intervals, and simultaneously allow developing an in vivo panel of sister lines differing for induced deletions across the Qfhs.ifa‐5A interval that can be used for phenotyping.
Juvenile elastoma without germline mutations in LEMD3 gene: A case of Buschke‐Ollendorff syndrome?
Authors: Condorelli Alessandra , Musso Nicolo , Scuderi Laura , Condorelli Daniele F. , Barresi Vincenza , De Pasquale Rocco 
Published: October 2017

AbstractWe report the case of a 6‐year‐old Caucasian girl with clinical and histopathologic features of Buschke‐Ollendorff syndrome. Histologic examination of skin lesions showed thick, curly, elastic fibers in the derma. Bone lesions compatible with Buschke‐Ollendorff syndrome were found in the girl's mother. Mutations in LEMD3 are pathogenic for Buschke‐Ollendorff syndrome. Analysis of all exons and exon‐intron junctions of LEMD3 did not reveal any germline mutations.
Pediatric keloids: A 6‐year retrospective review
Authors: Michael Afieharo I. , Ademola Samuel A. , Olawoye Olayinka A. , Iyun Ayodele O. , Adebayo Wasiu , Oluwatosin Odunayo M. 
Published: October 2017

AbstractBackground/ObjectivesKeloids are reportedly rare at the extremes of life. We sought to describe the epidemiology of pediatric keloids seen at the plastic surgery outpatient department of the University College Hospital, Ibadan.MethodsWe retrospectively reviewed all children younger than 19 years who presented with nonburn keloids between 2008 and 2014. Data were obtained on age; duration, size, and location of the keloid; family history; mode of treatment; and outcome. Outcome variables were recurrence and wound complications. Data were analyzed using the Pearson chi‐square test for discreet variables and the independent‐sample t test for continuous variables. P < .05 was taken as statistically significant.ResultsWithin the review period, 304 patients presented with keloids, of whom 40 (13.1%) were younger than 19 years. There was a female preponderance (n = 23, 57.5%). The mean age at onset of the keloid was 9.3 years (range 3 months‐18 years). Thirty (75%) patients had keloids in the head and neck region. Keloids were sporadic in 31 (77.5%) patients. Nineteen (47.5%) patients had multimodal treatment for keloid. The recurrence rate was 20%. Recurrence was significantly associated with the size of the lesion (P = .003).ConclusionKeloids during childhood are not rare. More attention should be paid to the management of keloids in this age group.
Perineal groove in female infants: A case series and literature review
Authors: Garcia‐Palacios Maria , Mendez‐Gallart Roberto , Cortizo‐Vazquez Jorge , Rodriguez‐Barca Pablo , Estevez‐Martinez Elina , Bautista‐Casasnovas Adolfo 
Published: October 2017

AbstractBackgroundPerineal groove is a rare congenital malformation that is unknown to many clinicians and is often misdiagnosed. Although it may be self‐resolving during childhood, this nonepithelized mucous membrane can pose the risk of local irritation and infection, particularly urinary tract infection.MethodsA retrospective study of female infants diagnosed with a perineal groove was performed, demographic characteristics and clinical features were analyzed, and a photographic review was conducted.ResultsFive patients with perineal groove were observed in our clinic in 2015‐16. The mean age was 14 months. None had symptoms, and no treatment was required. During follow‐up of 1 year, all remained asymptomatic.ConclusionsOur retrospective review suggests that perineal groove may be an underdiagnosed condition. Most cases resolve spontaneously, but confusion in diagnosis may lead to misdiagnosis or misinterpretation of sexual abuse and unnecessary treatments.