bluuebunny/crossref_metadata_2025_split huggingface dataset:
A metabolite of prostaglandin D2, 11β-prostaglandin F2α (11β-PGF2α), in exhaled breath condensate and serum of asthmatics with airway hyperresponsiveness to distilled water
Authors: Nekrasov Eduard V. , Perelman Juliy M. , Naumov Denis E. , Prikhodko Anna G. , Ushakova Elena V. , Kolosov Victor P. 
Published: March 2016

This study aims at identifying prostaglandin D2 (PGD2) involvement in osmotic airway hyperresponsiveness of asthmatics. PGD2 primary plasma metabolite, 11β-PGF2α, was analyzed in exhaled breath condensate (EBC) in response to ultrasonically nebulized distilled water (UNDW) and in serum in asthmatics with different airway response to the hypoosmotic stimulus. The total group of asthmatics (n=27) had a lower basal level of 11β-PGF2α (0.38±0.13 pg/ml, mean±SEM) in EBC compared to a group of healthy subjects (0.86±0.31 pg/ml, n=5), which decreased following the UNDW challenge to 0.30±0.09 and 0.53±0.12, respectively. The group of asthmatics with airway hyperresponsiveness to UNDW (≥10% FEV1 drop from baseline, n=14) had a lower concentration of the metabolite (0.28±0.14 pg/ml) as compared to the group without hyperresponsiveness (0.49±0.31 pg/ml, n=10). The 11β-PGF2α concentration decreased in the both groups after the challenge: 0.20±0.04 and 0.23±0.07 pg/ml in the groups with and without hyperresponsiveness to UNDW, respectively . Serum content of 11β-PGF2α was ranging from 0 to 61 pg/ml in asthmatics (n=17) and from 7.3 to 85.4 pg/ml in healthy subjects (n=8). It was lower in the group with airway hyperresponsiveness to UNDW (8.4±1.7 pg/ml, n=9) than in the group without the hyperresponsiveness (21.0±8.8 pg/ml, n=8). The obtained results do not support the involvement of PGD2 in the pathophysiology of asthma with airway hyperresponsiveness to a hypoosmotic stimulus unless other conversions of the prostaglandin occur in the airway under these conditions with formation of metabolites different from 11β-PGF2α.
A curated transcriptome dataset collection to investigate the development and differentiation of the human placenta and its associated pathologies
Authors: Marr Alexandra K. , Boughorbel Sabri , Presnell Scott , Quinn Charlie , Chaussabel Damien , Kino Tomoshige 
Published: March 2016

Compendia of large-scale datasets made available in public repositories provide a precious opportunity to discover new biomedical phenomena and to fill gaps in our current knowledge. In order to foster novel insights it is necessary to ensure that these data are made readily accessible to research investigators in an interpretable format. Here we make a curated, public, collection of transcriptome datasets relevant to human placenta biology available for further analysis and interpretation via an interactive data browsing interface. We identified and retrieved a total of 24 datasets encompassing 759 transcriptome profiles associated with the development of the human placenta and associated pathologies from the NCBI Gene Expression Omnibus (GEO) and present them in a custom web-based application designed for interactive query and visualization of integrated large-scale datasets (http://placentalendocrinology.gxbsidra.org/dm3/landing.gsp). We also performed quality control checks using relevant biological markers. Multiple sample groupings and rank lists were subsequently created to facilitate data query and interpretation. Via this interface, users can create web-links to customized graphical views which may be inserted into manuscripts for further dissemination, or e-mailed to collaborators for discussion. The tool also enables users to browse a single gene across different projects, providing a mechanism for developing new perspectives on the role of a molecule of interest across multiple biological states. The dataset collection we created here is available at: http://placentalendocrinology.gxbsidra.org/dm3.
Association of polymorphism rs7903146 gene TCF7L2 with low concentrations of autoantibodies in latent autoimmune diabetes of adults (LADA)
Authors: Silko Iuliia V. , Nikonova Tatiana V. , Ivanova Olga N. , Stepanova Svetlana M. , Shestakova Marina V. , Dedov Ivan I. 
Published: March 2016

Aim. To determine the frequencies of alleles and genotypes of polymorphic marker rs7903146 of the TCF7L2 gene in latent autoimmune diabetes in adults (LADA) and healthy individuals. The aims of the study were also to compare the distribution of alleles and genotypes and to explore the association with the development of LADA.Materials and methods. A total of 96 patients (46 females and 50 males) with LADA and 201 healthy individuals were examined. A quantitative determination of autoantibodies GADA, ICA, IA-2A and ZnT8 in the serum of LADA patients was performed. All patients underwent genotyping of rs7903146 of the TCF7L2 genes.Results. There was an increased frequency of the T allele and genotype T+ of marker rs7903146 of the TCF7L2 gene in patients with LADA with low concentrations of autoantibodies compared to a group of patients with high concentrations and with controls. We observed significant associations of the T allele and genotype T+ with LADA in patients with low concentrations of autoantibodies [p = 0.02; odds ratio (OR) = 1.85; 95% confidence interval (CI) = 1.10–3.13 and p = 0.04; OR = 2.14; 95% CI = 1.01–4.53 for the T allele and genotype T+, respectively).Conclusion. The results of the study suggest that LADA patients with low concentrations of autoantibodies have a genetically pre-determined similarity with patients with type 2 diabetes.
Pharmacogenetics of statin therapy and the endothelial function parameters in patients with type 2 diabetes mellitus
Authors: Lebedeva Nadezhda O. , Vikulova Olga K. , Nikitin Alexei G. , Shamkhalova Minara Sh. , Shestakova Marina V. , Dedov Ivan I. 
Published: March 2016

Aim. To investigate the association of variation in lipid-lowering response and endothelial function (EF) parameters after atorvastatin therapy in patients with type 2 diabetes mellitus (T2DM) with genetic markers of atherosclerosis.Methods. We included 97 patients with T2DM who were prescribed atorvastatin. Fasting lipid profiles and EF parameters were assessed before and after 12 months of statin therapy. For EF evaluation, we performed pulse-wave analysis during reactive hyperaemia. The genotypes for polymorphic markers were identified by real-time polymerase chain reaction with TaqMan probes. The statistical analysis included Wilcoxon, Mann–Whitney and Kruskal–Wallis tests. P-values 0.05 were considered statistically significant.Results. With statin therapy, PPARG2Pro/Pro patients had significantly lower TC and LDL-C levels than PPARG2 Pro/Ala and PPARG2 Ala/Ala patients (TC: 20.74% vs. 4.6% and 5.61%; p = 0.04 and LDL-C: 26.00% vs. 6.11% and 7.32%; p = 0.029). Patients with АРОЕЕ4/Е4 had significantly lower TC and TG levels than other АРОЕ patients (TC: -46.25% for Е4/Е4 vs. +33.33% for Е4/Е2, +5.73% for Е3/Е2, +11.80% for Е3/Е4, -10.92% for Е3/Е3, р = 0,01; TG: -56.52% for Е4/Е4 vs. +24.43% for Е4/Е2, +19.63% for Е3/Е2, +8.05% for Е3/Е4, -20.00% for Е3/Е3, р = 0.04). The patients with GG for TNFα G(238)A and GA for TNFα G(308)A had significantly greater amplitude of post-occlusive wave increase (Apw) than patients with GA for TNFα G(238)A and GG for TNFα G(308)A (+8.16 % vs. -0.93%, р = 0,04; +44% vs. -4.4%, p = 0.004, respectively).Conclusion. PPARG2Pro12Ala and АРОЕE2/Е3/Е4 polymorphism contributed to the between-patient variability in the response to statin therapy in patients with T2DM. Significant associations of the TNFαgene polymorphism with EF in patients with T2DM suggest an important role of inflammation in the pathogenesis of MVD.
Clinical and pathogenetic features of lesions of the lower extremities in patients with type 2 diabetes mellitus and chronic venous insufficiency
Authors: Shlyakova Anna A. , Strongin Leonid G. , Kudykin Maksim N. , Korneva Кsenia G. 
Published: March 2016

Comorbid chronic venous insufficiency (CVI) and type 2 diabetes mellitus (T2DM) are common, particularly in older people. The severity of DM and its complications can worsen the course of CVI and affect its management.Aim. To assess the impact of T2DM on lesions of the lower extremities in patients with CVI.Materials and methods. Eighty patients with CVI of the lower limbs were examined. Forty patients had T2DM (main group) and 40 patients did not have T2DM (control group). Physical examination, clinical and biochemical tests, ultrasound scanning of veins and arteries of the lower extremities and electroneuromyography (ENMG) of the lower extremities were performed for all patients. The state of the microvasculature was studied by laser Doppler flowmetry (LDF) for 15 patients in the main group and 15 patients in the control group.Results. T2DM exacerbated the course of CVI, which was clinically characterized by a greater severity of trophic (p = 0.0001) and oedema (p = 0.03) syndromes. Morphological changes in the venous blood flow in patients with T2DM with CVI were characterized by bilateral lesions (p = 0.03), more frequent failure of sapheno-femoral anastomosis (p = 0.02) and perforating veins of the lower leg (p = 0.0004). The pathogenesis of such complications was associated with diabetic factors, including hyperglycaemia, НbА1с 10%, duration of DM 10 years and the presence diabetic microangiopathy of the lower limbs. Diabetic macroangiopathy and polyneuropathy were associated with disruption of the morphological and functional characteristics of the venous system and the disruption of the microcirculation in the lower extremities, contributing to increased oedema and trophic changes. At the same time, the presence of diabetic neuropathy masked the symptoms of CVI due to reductions in pain (p = 0.0004).Conclusion. Diabetes exacerbates the course of CVI due to poor glycaemic control (HbA1c 10%), long duration of diabetes (10 years) and the presence of macroangiopathy in the lower extremities. Diabetic neuropathy of the lower limbs and diabetic microangiopathy aggravates the venous disease through disruption of microcirculation and increases the expression of trophic changes in the lower extremities.
The associations of incretin hormone concentration with gestational diabetes mellitus
Authors: Saprina Tatiana V. , Timokhina Ekaterina S. , Goncharevich Olga K. , Budeeva Svetlana V. , Prokhorenko Tatiana S. , Tashireva Lyubov A. , Musina Nadezhda N. , Dronova Irina V. 
Published: March 2016

Background: Enteropancreatic hormonal system disorder is a possible reason for β-cell dysfunction and carbohydrate metabolism disorder among pregnant women. However, no information is available about the state of enteroinsulin hormones [glucagon, glucose-dependent insulinotropic peptide (GIP), glucagon-like peptide1 (GLP-1) and GLP-2] during pregnancy. The role of enteroinsulin hormones in the development of carbohydrate metabolism disorder during pregnancy is poorly understood. Aim: To quantify and compare incretin hormone secretion in groups of pregnant women with and without gestational diabetes mellitus (GDM). Materials and methods: The study included 80 patients, 50 of whom had GDM, and the control group consisted of 30 pregnant women without GDM. All patients underwent an oral glucose tolerance test; glycated haemoglobin (HbA1c) estimation; ferritin, transferrin, basal and postprandial glucagon estimation; GLP-1 and GLP-2 estimation. Results: Basal glucagon and GLP-1 levels were significantly higher (p 0. 05) in the group of women with GDM than in the control group. The most significant differences in GLP-1, basal and postprandial glucagon levels were observed during the first trimester of pregnancy. Conclusion: High GLP-1 levels in the group of women with GDM may reflect a state of ‘incretin resistance’, which is similar to hyperinsulinemia in the early stages of type 2 diabetes mellitus.
Phenotypic and genetic characteristics of patients with type 2 diabetes with different responses to metformin therapy in Novosibirsk region
Authors: Bondar Irina A. , Shabelnikova Olesya Y. , Sokolova Ekaterina A. , Pyankova Olga V. , Filipenko Maksim L. 
Published: March 2016

Aim: The purpose of this study was to examine the phenotypic and genetic characteristics of patients with type 2 diabetes mellitus (T2DM) with different responses to treatment with metformin (MF) in the Novosibirsk region. Materials and methods: We examined 460 patients with T2DM in the Novosibirsk region. Patients were divided into groups according to their HbA1c level: patients who achieved the target HbA1c level during MF therapy (n = 209) and those who did not reach the target HbA1c level (n=251). Genotyping of ATM (rs11212617) was performed using polymerase chain reaction by TaqMan. Results: Patients who achieved the target HbA1c level during MF treatment (good response) were older (61. 1±9. 1 years vs. 57. 4±8. 4 years, p=0. 001), had later onset of diabetes (54. 6 ± 10. 1 years vs. 49. 2±8. 5 years, p = 0. 0001) and shorter duration of diabetes (6. 5±5. 9 years vs. 8. 2±6. 1 years, p=0. 03) compared with those who did not achieve the target HbA1c level. There was no statistically significant association between ATM rs11212617 and achieving the target HbA1c level among all patients [odds ratio (OR)=0. 94, 95% confidence interval = (0. 73–1. 23), p=0. 67] or those with MF monotherapy [OR=0. 90, (0. 65–1. 25), p=0. 54] or combination therapy [OR=1. 02, (0. 72–1. 43), p=0. 92]. There was an effect of age on response to MF therapy in all three groups (all patients: p=0. 001, MF monotherapy group: p=0. 04, combination therapy group: p=0. 0009). In the MF monotherapy group, low dose MF was associated with a good response (p=0. 03), and in the combination therapy group, males were more likely to have a good response (p=0. 003). Patients with genotype C/C or A/C for ATM (rs11212617) compared with those with genotype A/A were more likely to have high levels of triglycerides [2. 33 (1. 52–4. 2) mmol/l, 2. 09 (1. 35–3. 0) mmol/l and 1. 99 (1. 49–3. 21) mmol/l, respectively, p=0. 001], coronary heart disease (CHD) (13. 4%, 13. 4% and 9. 6%, respectively, p=0. 009) and myocardial infarction (7. 8%, 3. 2% and 4. 0%, respectively, p=0. 001). Conclusion: Patients with T2DM who had a good response to MF therapy were older, more likely to be male and had a later onset of T2DM. Genotype C/C for ATM rs11212617 was associated with high triglycerides, CHD and myocardial infarction. ATM rs11212617 was not associated with response to MF therapy in the Novosibirsk region.
Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems
Authors: Ciekot Jarosław , Goszczyński Tomasz Marek , Boratyński Janusz 
Published: March 2016

Abstract
Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 μmol L−1 PMX. The limit of quantitation was 1.070 μmol L−1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 μmol L−1. LOQ was 0.539 μmol L−1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles.
Synthesis and structure elucidation of some novel thiophene and benzothiophene derivatives as cytotoxic agents
Authors: Mohareb Rafat M. , Abdallah Amira E. M. , Helal Maher H. E. , Shaloof Somiya M. H. 
Published: March 2016

Abstract
Attempting to produce cyclized systems with potential anti-proliferative activity, a series of novel thiophene and benzothiophene derivatives were designed and synthesized. The reactivity of the latter derivatives towards different chemical reagents was studied. Twenty-one compounds were synthesized and evaluated as anti-cancer agents. The results showed that ethyl 5-amino-3-(4-chlorostyryl)-4-cyanothiophene-2-carboxylate (5b), ethyl 5-amino-4-((4-methoxyphenyl)carbonyl)-3-methylhiophene-2-carboxylate (8c) and 5-3-(ethoxy-3-oxopropanamido)-3-methyl-4-(phenylcarbamoyl)thiophene-2-carboxylate (9) were the most active compounds towards three tumor cell lines – MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer) and SF-268 (CNS cancer) and a normal fibro-blast human cell line (WI-38) compared to the anti-proliferative effects of the reference control doxorubicin.
Biphasic dissolution method for quality control and assurance of drugs containing active substances in the form of weak acid salts
Authors: Franc Aleš , Muselłk Jan , Goněc Roman , Vetchý David 
Published: March 2016

Abstract
Substances in the form of weak acid salts have been found to be problematic for dissolution testing. Their absorption can start only after they are turned into the form of an acid following the gastric passage although they were administered in the form of a salt. Due to poor solubility, they cannot be tested in acidic gastric environment for a biased dissolution profile. The biphasic dissolution method is promising for overcoming this obstacle. Tablets with warfarin clathrate sodium salt in two concentrations and two different particle size distributions were tested as a suitable model for finding the medium and process conditions of dissolution. The dissolution method based on the use of the upper organic layer (1-octanol) and the lower aqueous layer 0.1 mol L−1 HCl) was found suitable and discriminatory for tablets containing active substances in the form of salts of weak acids. The method also reflects physical differences in the quality of used substances.
Numerical Method for Determining Stiffness Characteristics of an Arbitrary Form Superelement
Authors: Tsybenko Alexander , Konyukhov Alexander , Tsybenko Hanna 
Published: March 2016

Abstract
As part of the superelement approximation technology for fragments (subsystems) of the analyzed structures, a numerical method of determining the characteristics of arbitrary type superelements was developed. The examples of simulation models with two-node superelements demonstrated the efficacy of the method in the structural analysis of elastic systems.
Lusin-type Theorems for Cheeger Derivatives
on Metric Measure Spaces
Authors: Guy C. David  
Published: March 2016

Abstract
A theorem of Lusin states that every Borel function onRis equal almost everywhere to the derivative
of a continuous function. This result was later generalized to Rn in works of Alberti and Moonens-Pfeffer.
In this note, we prove direct analogs of these results on a large class of metric measure spaces, those with
doubling measures and Poincaré inequalities, which admit a form of differentiation by a famous theorem of
Cheeger.
Analysis of Correlation between Stresses and Fatigue Lives of Welded Steel Specimens Based on Real Three-Dimensional Weld Geometry
Authors: Stasiuk Przemysław , Karolczuk Aleksander , Kuczko Wiesław 
Published: March 2016

Abstract
Welded joints are areas of increased stresses in construction. The reason for this phenomenon is associated with the nonhomogeneous mechanical, structural and geometrical properties of the weld. In the article the correlations between locally raised stresses due to real geometry and fatigue lives of non-load carrying cruciform joints made from S355J2+N steel are analysed. Stresses were computed using Finite Element Method (FEM) based on real three-dimensional weld geometry obtained by 3D scanning. The specimens were experimentally tested under cyclic push-pull loading with a zero mean value of applied force. The correlation was analysed using Pearson’s correlation coefficient and statistical hypotheses. It was shown that statistically significant correlation exists between maximum values of normal stresses and fatigue lives.
Some Considerations on an Underwater Robotic Manipulator Subjected to the Environmental Disturbances Caused by Water Current
Authors: Kołodziejczyk Waldemar 
Published: March 2016

Abstract
The objective of this paper is to discuss some of the issues associated with environmental load on the three-link serial manipulator caused by underwater current. We have conducted CFD simulations to investigate hydrodynamic effects induced by changing current direction and changing with time current speed in order to better understand the physics of the problem. The results are presented in terms of moments of hydrodynamic forces plotted against relative position of the current and the robotic arm. Time history of hydrodynamic loads according to periodically changing current speed is presented and discussed.
Thrust Porous Bearing with Rough Surfaces Lubricated by a Rotem-Shinnar Fluid
Authors: Walicka Anna , Walicki Edward 
Published: March 2016

Abstract
In the paper the influence of both bearing surfaces roughness and porosity of one bearing surface on the pressure distribution and load-carrying capacity of a thrust bearing surfaces is discussed. The equations of motion of a pseudo-plastic fluid of Rotem-Shinnar, are used to derive the Reynolds equation. After general considerations on the flow in a bearing clearance and in a porous layer using the Morgan-Cameron approximation and Christensen theory of hydrodynamic lubrication the modified Reynolds equation is obtained. The analytical solutions of this equation for the cases of squeeze film bearing and externally pressurized bearing are presented. As a result one obtains the formulae expressing pressure distribution and load-carrying capacity. Thrust radial bearing with squeezed film is considered as a numerical example.
Boundary Integral Equations for an Anisotropic Bimaterial with Thermally Imperfect Interface and Internal Inhomogeneities
Authors: Sulym Heorhiy , Pasternak Iaroslav , Tomashivskyy Mykhailo 
Published: March 2016

Abstract
This paper studies a thermoelastic anisotropic bimaterial with thermally imperfect interface and internal inhomogeneities. Based on the complex variable calculus and the extended Stroh formalism a new approach is proposed for obtaining the Somigliana type integral formulae and corresponding boundary integral equations for a thermoelastic bimaterial consisting of two half-spaces with different thermal and mechanical properties. The half-spaces are bonded together with mechanically perfect and thermally imperfect interface, which model interfacial adhesive layers present in bimaterial solids. Obtained integral equations are introduced into the modified boundary element method that allows solving arbitrary 2D thermoelacticity problems for anisotropic bimaterial solids with imperfect thin thermo-resistant inter-facial layer, which half-spaces contain cracks and thin inclusions. Presented numerical examples show the effect of thermal resistance of the bimaterial interface on the stress intensity factors at thin inhomogeneities.
Chaotic Dynamics of the Kepler Problem with Oscillating Singularity
Authors: Margheri Alessandro , Torres Pedro J. 
Published: March 2016

Abstract
We prove the presence of chaotic dynamics for the classical two-body Kepler problem with a time-periodic gravitational coefficient oscillating between two fixed values. The set of chaotic solutions we detect is coded by the number of revolutions in each period. The chaotic dynamics is obtained for large period T as well as for small angular momentum μ. In particular, we provide an explicit lower bound on T and explicit upper bound on μ which guarantee the existence of complex dynamics. We get our results by applying a simple and well-known topological method, the stretching along the path technique. Our results are robust with respect to small perturbations of the gravitational coefficient and to the addition of a small friction term.