bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between SomaticPIK3CAMutations and Ancestry in South Asia: Prognostic Insights From a Sri Lankan Breast Cancer Study
Authors: Cabraal Tharini Ruwinya , Kumarasinghe Iranthi , Perera Ranga , Balawardane Jayantha , Viswakula Sameera , Perera Nandika , Galhena Gayani Harendra 
Published: October 2024

AbstractBackgroundThePIK3CAoncogene is one of the most mutated oncogenes in breast cancer, with ancestry-linked variations reported globally. The study aimed to discern the prevalence and prognostic role ofPIK3CAmutations in Sri Lankan breast cancer patients for the first time, focusing on the correlation between somatic mutations and patient ancestry in an exclusively South Asian cohort of breast cancers.Materials & MethodsA qPCR-based genetic analysis was performed on DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples of 63 clinically diagnosed, female, Sri Lankan breast cancer patients using the QClamp®PIK3CAMutation Detection Test for the hotspot mutations ofPIK3CA(i.e., H1047R, E545K, E542K) followed by a statistical analysis. Patient samples and clinical data were fully anonymized, with no identifying information available to authors at any point of the study.ResultsSomatic missensePIK3CAmutations H1047R and E542K, were detected in 17.46% of the cohort. E545K mutation was not detected. The observed mutations were associated with an increased risk of lymph node (LN) metastasis (p=0.036, OR 9.60) and reduced recurrence-free survival (RFS) (p<0.001, HR 26.19). In addition, LN metastasis (p=0.026, HR 123.94) and a high Ki67 index (p=0.029, HR 79.69) were individually associated with reduced RFS. All three factors above-presence of aPIK3CAmutation, LN metastasis and a high Ki67 index-in combination, were also associated with reduced RFS (p<0.001). Further analysis revealed a significant association between patient ancestry andPIK3CAmutation status (p=0.028).ConclusionDespite being a pilot study, the findings suggest thatPIK3CAmay serve as a potential prognostic biomarker in Sri Lankan breast cancer patients, with ancestry-linked variations potentially influencing metastatic outcomes. These results bring out the importance of integrating PI3K inhibitors into the therapeutic management of breast cancers in Sri Lanka after validating these findings in a functional study using a larger cohort.
Assessing how women access healthcare to inform cervical cancer and HIV screening in rural Uganda
Authors: Sheehan Mia , Dau Hallie , AboMoslim Maryam , Naguti Priscilla , Mwandacha Nelly , Booth Amy , Ruck Candice , Smith Laurie , Orem Jackson , Ogilvie Gina , Nakisige Carolyn 
Published: October 2024

ABSTRACTObjectiveThis study aims to compare how HIV-positive and HIV-negative women in a remote sub-country in Uganda access health services to inform consideration of potential HIV and HPV-based cervical cancer screening integration at the community level.MethodsThis cross-sectional study recruited women living in the South Busoga District Reserve from January to August 2023. Women were eligible if they were aged 30 to 49 years old, had no history of cervical cancer screening or treatment, had no previous hysterectomy, and could provide informed consent. Participants completed a survey administered by village health teams, which included questions on HIV status, demographics, healthcare access, and services received. The data was analyzed using bivariate descriptive statistics, including chi-square and Fisher’s exact tests.ResultsAmong the 1437 participants included in the analysis, 8.8% were HIV-positive. The majority of the respondents were between 30-39 years of age, were married, had received primary education or higher, and were farmers. The majority of women in both groups had accessed outreach visits (HIV-positive = 89.0%, HIV-negative = 85.8%) and health centres (HIV-positive = 96.1%, HIV-negative = 80.2%). The most commonly received services among both groups of women at outreach visits and health centres were immunization and antenatal care, respectively.ConclusionOur study demonstrated that there were no significant differences in healthcare access between HIV-positive and HIV-negative women in rural Uganda. Additionally, the high usage of healthcare services by women living with HIV suggests that the integration of cervical cancer and HIV screening may facilitate early detection and prevention of cervical cancer among this population. This can reduce the burden of disease in Uganda and further contribute to the World Health Organization’s initiative to eradicate cervical cancer.
Protocol for evaluation of sustained virological response as a surrogate outcome for mortality, decompensated cirrhosis, or hepatocellular carcinoma in people with chronic hepatitis C virus infection treated with direct-acting antivirals
Authors: Gurusamy Kurinchi , Gluud Christian 
Published: October 2024

AbstractIntroductionSustained virological response (SVR) is commonly used as an indicator of treatment success in people with chronic hepatitis C virus (HCV) infection. However, there is uncertainty on whether SVR is a validated surrogate marker of successful treatment of chronic HCV infection.AimTo evaluate whether SVR is a good surrogate for all-cause mortality, decompensated cirrhosis, or any specific aspect of liver decompensation (jaundice, ascites, hepatic encephalopathy, hepatorenal syndrome, or variceal haemorrhage), or hepatocellular carcinoma in people with chronic HCV infection eligible to receive direct-acting antiviral drugs.MethodsData sourceTwo ongoing systematic reviews on the effectiveness of direct-acting antiviral drugs in chronic HCV infection.AnalysisEstimate the regression coefficients or between-studies correlation between SVR and the event by three different Bayesian approaches with OpenBUGS, as outlined in the guidance by the Evidence Synthesis Unit (Technical support document 20).Estimate the average proportion of the effect mediated through SVR by causal mediation analysis using R.DiscussionWe will use the German Institute of Quality and Efficiency in Health Care (IQWiG) criterion for surrogacy for cancer and at least 50% of the treatment effect mediated through SVR but will report the information in a way that allows people to interpret the information using their own criteria.
Dysregulated balance of D- and L-amino acids modulating glutamatergic neurotransmission in severe spinal muscular atrophy
Authors: Hassan Amber , di Vito Raffaella , Nuzzo Tommaso , Vidali Matteo , Carlini Maria Jose , Yadav Shubhi , Yang Hua , D’Amico Adele , Kolici Xhesika , Valsecchi Valeria , Panicucci Chiara , Pignataro Giuseppe , Bruno Claudio , Bertini Enrico , Errico Francesco , Pellizzoni Livio , Usiello Alessandro 
Published: October 2024

AbstractSpinal muscular atrophy (SMA) is a neuromuscular disorder caused by reduced expression of the survival motor neuron (SMN) protein. In addition to motor neuron survival, SMN deficiency affects the integrity and function of afferent synapses that provide glutamatergic excitatory drive essential for motor neuron firing and muscle contraction. However, it is unknown whether deficits in the metabolism of excitatory amino acids and their precursors contribute to neuronal dysfunction in SMA. To address this issue, we measured the levels of the main neuroactive D- and L-amino acids acting on glutamatergic receptors in the central nervous system of SMNΔ7 mice as well as the cerebrospinal fluid (CSF) of SMA patients of varying severity before and after treatment with the SMN-inducing drug Nusinersen. Our findings reveal that SMN deficiency disrupts glutamate and serine metabolism in the CSF of severe SMA patients, including decreased concentration of L-glutamate, which is partially corrected by Nusinersen therapy. Moreover, we identify dysregulated L-glutamine to L-glutamate conversion as a shared neurochemical signature of altered glutamatergic synapse metabolism that implicates astrocyte dysfunction in both severe SMA patients and mouse models. Lastly, consistent with a correlation of higher CSF levels of D-serine with better motor function in severe SMA patients, we show that daily supplementation with the NMDA receptor co-agonist D-serine improves neurological deficits in SMNΔ7 mice. Altogether, these findings provide direct evidence for dysregulation of D- and L-amino acid metabolism linked to glutamatergic neurotransmission in severe SMA and have potential implications for treating this neurological disorder.
Engineered protein destabilization reverses intrinsic immune evasion for candidate vaccine pan-strain KSHV and SARS-CoV-2 antigens
Authors: Wan Li , Xie Bingxian , Shuda Masahiro , Delgoffe Greg , Chang Yuan , Moore Patrick S. 
Published: October 2024

AbstractBoth Kaposi sarcoma herpesvirus LANA and SARS coronavirus 2 RdRp/nsp12 are highly conserved replication proteins that evade immune processing. By deleting the LANA central repeat 1 domain (LANAΔCR1) or by dividing RdRp into two separated fragments (RdRpFrag) to maximize nascent protein mis-folding, cis peptide presentation was increased. Native LANA or RdRp SIINFEKL fusion proteins expressed in MC38 cancer cells were not recognized by activated OT-1 CD8+cells against SIINFEKL but cytotoxic recognition was restored by expression of the corresponding modified proteins. Immunocompetent syngeneic mice injected with LANA- or RdRp-SIINFEKL MC38 cells developed rapidly-growing tumors with short median survival times. Mice injected with LANAΔCR1- or RdRpFrag-SIINFEKL had partial tumor regression, slower tumor growth, longer median survival, as well as increased effector-specific tumor-infiltrating lymphocytes. These mice developed robust T cell responses lasting at least 90 days post-injection that recognized native viral protein epitopes. Engineered vaccine candidate antigens can unmask virus-specific CTL responses that are typically suppressed during native viral infection.
Singular viscoelastic perturbation to soft lubrication
Authors: Bharti  , Ferreira Quentin , Jha Aditya , Carlson Andreas , Dean David S. , Amarouchene Yacine , Chan Tak Shing , Salez Thomas 
Published: October 2024

Soft lubrication has been shown to drastically affect the mobility of an object immersed in a viscous fluid in the vicinity of a purely elastic wall. In this theoretical article, we develop a minimal model incorporating viscoelasticity, carrying out a perturbation analysis in both the elastic deformation of the wall and its viscous damping. Our approach reveals the singular-perturbation nature of viscoelasticity to soft lubrication. Numerical resolution of the resulting nonlinear, singular, and coupled equations of motion reveals peculiar effects of viscoelasticity on confined colloidal mobility, opening the way towards the description of complex migration scenarios near realistic polymeric substrates and biological membranes.

Published by the American Physical Society
2024
Advancing open education through open-source software: examining UTAUT 2 factors in adoption and implementation
Authors: Duan Chenggui 
Published: October 2024

PurposeThis study aims to examine the factors influencing university students' adoption of open-source software (OSS) within the context of open educational practices (OEP) by applying an extended Unified Theory of Acceptance and Use of Technology (UTAUT 2) model.Design/methodology/approachThe research employs a quantitative approach, gathering data from 156 students at Hong Kong Metropolitan University through an online survey. The survey was designed to test nine hypotheses derived from the UTAUT 2 model, incorporating additional constructs relevant to OSS. Structural equation modelling (SEM) was used to analyse the data and test the relationships between constructs.FindingsThe results indicate that Performance Expectancy (PE), Effort Expectancy (EE), Price Value (PV), Self-Efficacy (SE) and Value Alignment (VA) significantly influence students' Behavioral Intention (BI) to adopt OSS. Conversely, Social Influence (SI), Facilitating Conditions (FC), Hedonic Motivation (HM) and Habit (HT) were not significant predictors. The findings suggest that while UTAUT 2 provides a useful framework for understanding OSS adoption, it requires adaptation to fully capture the unique characteristics of OSS in educational settings.Originality/valueThis study contributes to the literature by extending the UTAUT 2 model to the context of OSS adoption in higher education, highlighting the importance of economic factors and user alignment with OSS values. The results offer practical insights for higher education institutions aiming to promote OSS, emphasizing the need for support structures, training, and the promotion of OSS’s economic and collaborative benefits.
Nondestructive differential eddy current testing for corrosion detection on coated aluminium alloys
Authors: Tang Xiaoliang , Zhou Jun , Jian Guangjian , Deng Qingzhu , Zhao Wen , Mo Shaolan , She Zuxin , Zhong Yong , Huang Lun , Shu Chang , Pan Maolin , Wang Zhongwei 
Published: October 2024

Purpose
The objective of this study is to use non-destructive testing of corrosion on coated aluminium alloys using differential eddy current detection (DECD), with the aim of elucidating the relationship between the characteristics of corrosion defects and the detection signal.

Design/methodology/approach
Pitting corrosion defects of varying geometrical dimensions were fabricated on the surface of aluminium alloy plates, and their impedance signals were detected using DECD to investigate the influence of defect diameter, depth, corrosion products and coating thickness on the detection signals. Furthermore, finite element analysis was used to ascertain the eddy current distributions and detection signals under different parameters.

Findings
The size of the defect is positively correlated with the strength of the detection signal, with the defect affecting the latter by modifying the distribution and magnitude of the eddy current. An increase in the diameter and depth of corrosion defects will enhance the eddy current detection (ECD) signal. The presence of corrosion products in the corrosion defects has no significant effect on the eddy current signal. The presence of a coating results in a decrease in the ECD signal, with the magnitude of this decrease increasing with the thickness of the coating.

Originality/value
The objective is to provide experimental and theoretical references for the design of eddy current non-destructive testing equipment and eddy current testing applications.
Towards the fair regulation of all coca leaf derivatives in Colombia
Authors: Snapp Zara , Quintero Julián 
Published: October 2024

Purpose
The purpose of this study is to envision the possible path to regulation of the coca leaf and its derivatives. There are a range of possible coca derivatives that could be considered for the broader market and which could be conceived of as risk and harm reduction interventions, as well as have other nutritional and industrial uses.

Design/methodology/approach
The methodology consists of a historical analysis of the advances in alternative uses of the coca leaf within a commercial market in Colombia as a means of providing greater economic capacity to communities that cultivate the plant, while exploring the nutritional and industrial benefits of the plant.

Findings
Low-risk products, such as coca leaf and coca tea, could be regulated under the same criteria as coffee or other caffeinated beverages. With a more diverse range of products on the market, consumers can make more informed decisions about their consumption, particularly regarding the associated risks and the effects they desire.

Practical implications
The legal regulation of coca leaf products implies the creation of a regulatory government agency specialized in the subject, as well as monitor and evaluate the cultivation and processing of products. The production of the coca leaf would still be in the hands of the cultivating communities but would fall under the control and monitoring of the government.

Social implications
The legal regulation of the coca leaf could have strong impacts on cultivating communities to guarantee their human rights and provide greater protection. Various industries have expressed an interest in using coca leaf derivatives and could represent economic opportunities for communities that cultivate.

Originality/value
Minimal work has been done on the regulation of coca leaf although there are some international examples that could be relevant in Bolivia and Perú. There is a need to continue exploring the multiple uses of the leaf to reach greater international consensus on its rescheduling.
Examining how consumers can love brands through internal storytelling
Authors: Sarkar Abhigyan , Sarkar Juhi Gahlot , Dandotiya Gunjan 
Published: October 2024

Purpose
This research aims to empirically test the boundary conditions under which the archetype-based narrative structure of storytelling brand advertisement through internal endorsement can generate brand love by making the consumers anthropomorphize the brand archetype.

Design/methodology/approach
Two studies based on survey and experimentally designed stimuli were conducted to validate the conceptual framework. The data were analyzed using confirmatory factor analysis and process macro on SPSS.

Findings
The results support that when the brand is endorsed in the storytelling ad by an internal endorser or storyteller, the narrative structure of the storytelling ad impacts brand love via the anthropomorphization of the brand archetype. The congruence between the internal storyteller’s personality and the archetype’s personality moderates the effect of the narrative structure on the anthropomorphization of the brand archetype, leading to brand love. The psychological sense of brand community further conditions the moderating impact of congruence.

Originality/value
The research provides insights to the brand marketers that internally endorsed archetype-based storytelling brand ads can be effective when the personality of the internal endorser is congruent with the personality of the archetype and the target consumers have a strong psychological sense of brand community.
Analysis of intangible assets reporting standards and automation in KSA within an Islamic context – a case study
Authors: Bamhdi Alwi M. 
Published: October 2024

Purpose
This paper aims to the significance of intangible assets in boosting financial credibility and accounting transparency in Saudi Arabia and other Islamic countries, aligning with Saudi Vision 2030. Moreover, it articulates the indispensable role of information and communication technology (ICT), including cutting-edge technologies such as artificial intelligence (AI) and blockchain, inefficaciously mitigating manual challenges while safeguarding the authenticity and reliability of financial information.

Design/methodology/approach
This study examines relevant literature on national and international standards to define the significance of intangible assets within conventional and Islamic Shari’ah principles in the Kingdom of Saudi Arabia (KSA). It delves into how automation technologies can improve accuracy and verifiability in reporting intangible assets while adhering to Islamic Shari’ah Compliance (ISC) principles, providing valuable insights into standardization and automation benefits.

Findings
The study highlights that ISC laws affect intangible asset reporting. Integrating conventional accounting principles with ISC is necessary to establish a unified international standard. Adopting ICT, AI and blockchain is crucial for automating and ensuring consistent, verifiable reporting of intangible assets.

Research limitations/implications
This case study highlights the importance of intangible assets in KSA, but its findings require validation through workshops with scholars, stakeholders and policymakers to ensure broader acceptance and applicability beyond the specific context.

Practical implications
This study provides guidance on using AI, blockchain and other ICT tools to ensure accurate financial reporting by validating and verifying intangible assets, benefiting stakeholders, policymakers, accountants, auditors and ISC professionals.

Originality/value
This study underscores the importance of reporting intangible assets according to Islamic principles in KSA, with relevance to other Islamic countries. Promoting International Financial Reporting Standards and Saudi Organization for Certified Public Accountants standards enhances accurate reporting and supports Saudi Vision 2030 goal of boosting global investment and trade.
Gender inequality in the public sphere as perceived by Muslim female graduate students at a Pakistani higher education institution – a critical grounded theory perspective
Authors: Rashid Amber Gul , Asif Zaheeruddin 
Published: October 2024

Purpose
The subject of gender inequality has been approached by academics of various fields – psychologists, political scientists, developmental economists, feminists, sociologists, among others. Although there is a considerable amount of evidence indicating the presence of gender bias in various aspects of educational and work life in Muslim countries, not many studies have attempted to understand the female perspective during extra- and co-curricular activities in higher education institutions (HEIs) and their coping strategies.

Design/methodology/approach
A critical grounded theory approach was used.

Findings
The findings indicate that the role of female “religio-socio-cultural” stereotypes mutually reinforces each other to perpetuate an uneven playing field. Females have evolved many strategies to cope, including creation of sub-private spheres within larger public spheres and restricting their interactions to specific strata within the public sphere.

Originality/value
The study provides a female perspective regarding participation during extra- and co-curricular activities in a HEI in a Muslim majority context using critical grounded theory.
Self-presentational concerns and lurking among users on social networking sites: an empirical study based on a moderated mediation model
Authors: Bao Zheshi 
Published: October 2024

Purpose
The phenomenon of nonposting behavior, known as lurking, has become increasingly prevalent on social networking sites (SNS). This study aims to understand why certain users are inclined to lurk on SNS by proposing a theoretical framework that integrates self-presentational concerns, SNS fatigue and social presence.

Design/methodology/approach
Building upon the theoretical framework, a moderated mediation model is established to illustrate the mechanisms of lurking on SNS. Survey data were collected from 616 SNS users through an online survey and analyzed using the SPSS macro PROCESS.

Findings
The findings show that self-presentational concerns have positive and direct effects on lurking. Moreover, the relationship between self-presentational concerns and lurking is partially mediated by SNS fatigue. Furthermore, both the direct effect and the mediating effect are moderated by social presence.

Originality/value
This study offers a novel theoretical perspective on lurking behavior by introducing a moderated mediation model. The findings reveal intricate mechanisms underlying this specific SNS usage behavior and its connections to both self-presentational concerns and SNS fatigue, thereby enriching the existing literature on user engagement and inactivity on SNS. Furthermore, this research highlights the pivotal role of social presence in moderating the effects of self-presentational concerns, offering new insights into the dynamics of online social interactions.