bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the Weddell Sea Anomaly Using Novel Satellite Altimeter TEC Maps
Authors: Azpilicueta F. , Nava B. 
Published: October 2024

AbstractThe Weddell Sea Anomaly (WSA) is a phenomenon of unique intensity and geographic extent that occurs in December (Southern Hemisphere summer) over the southeastern Pacific and southwestern Atlantic oceans regions. Historically, the classic definition of the WSA refers to a situation in which the midnight NmF2 (or TEC) values are greater than the noon NmF2 (or TEC) values. However, several articles published in the last decades have shown that the WSA is a much more complex phenomenon, and its definition might need to be reformulated. This paper presents a phenomenological description of the WSA using a novel type of vertical Total Electron Content (TEC) maps, obtained from altimeter satellite TEC data. The focus of this study is on the possible connection between the WSA and the unexpected expansion and contraction periods observed on the Equatorial Ionospheric Anomaly (EIA) throughout the Southern Hemisphere. The data analysis revealed that the WSA is only one of a number of observed anomalies. Furthermore, we show a significant correlation between the behavior of the EIA and the Y component of the geomagnetic field, which maximizes in the WSA region. We then present a possible hypothesis for interpreting these results.
Proteomic biomarkers of long-term lung function decline in textile workers: a 35-year longitudinal study
Authors: Zhao Mengsheng , Wei Liangmin , Zhang Longyao , Hang Jingqing , Zhang Fengying , Su Li , Wang Hantao , Zhang Ruyang , Chen Feng , Christiani David C. , Wei Yongyue 
Published: October 2024

Abstract
Background
Occupational exposures contribute significantly to obstructive lung disease among textile workers. However, biomarkers associated with such declines are not available.

Objectives
We conducted a large-scale proteomic study to explore protein biomarkers potentially associated with long-term lung function decline.

Methods
Shanghai Textile Workers Cohort was established in 1981 with 35 years of follow-up, assessing textile workers’ lung functions every five years. Quantitative serum proteomics was performed on all 453 workers at 2016 survey. We employed four distinct models to examine the association between forced expiratory volume in one second (FEV1) and proteins, and consolidated the findings using an aggregated Cauchy association test. Furthermore, proteomic data of UK Biobank (UKB) was used to explore the associations of potential protein markers and decline of FEV1, and the interactions of these proteins were examined through STRING database. Associations were also externally validated using two-sample Mendelian randomizations (MR).

Results
15 of 907 analyzed proteins displayed potential associations with long-term FEV1 decline, including two hemoglobin subunits: hemoglobin subunit beta (HBB, FDR-qACAT = 0.040), alpha globin chain (HBA2, FDR-qACAT = 0.045), and four immunoglobulin subunits: immunoglobulin kappa variable 3–7 (IGKV3-7, FDR-qACAT = 0.003), immunoglobulin heavy chain variable region (IgH, FDR-qACAT = 0.011). Five proteins were significantly associated with the rate of decline of FEV1 in UKB, in which RAB6A, LRRN1, and BSG were also found to be associated with proteins identified in Shanghai Textile Workers Cohort using STRING database. MR indicated bidirectional associations between HBB and FEV1 (P < 0.05), while different immunoglobulin subunits exhibited varying associations with FEV1.

Impact statement
We performed a large-scale proteomic study of the longest-follow-up pulmonary function cohort of textile workers to date. We discovered multiple novel proteins associated with long-term decline of FEV1 that have potential for identifying new biomarkers associated with long-term lung function decline among occupational populations, and may identify individuals at risk, as well as potential pharmaceutical targets for early intervention.
Minimal exposure durations reveal visual processing priorities for different stimulus attributes
Authors: Lanfranco Renzo C. , Canales-Johnson Andrés , Rabagliati Hugh , Cleeremans Axel , Carmel David 
Published: October 2024

AbstractHuman vision can detect a single photon, but the minimal exposure required to extract meaning from stimulation remains unknown. This requirement cannot be characterised by stimulus energy, because the system is differentially sensitive to attributes defined by configuration rather than physical amplitude. Determining minimal exposure durations required for processing various stimulus attributes can thus reveal the system’s priorities. Using a tachistoscope enabling arbitrarily brief displays, we establish minimal durations for processing human faces, a stimulus category whose perception is associated with several well-characterised behavioural and neural markers. Neural and psychophysical measures show a sequence of distinct minimal exposures for stimulation detection, object-level detection, face-specific processing, and emotion-specific processing. Resolving ongoing debates, face orientation affects minimal exposure but emotional expression does not. Awareness emerges with detection, showing no evidence of subliminal perception. These findings inform theories of visual processing and awareness, elucidating the information to which the visual system is attuned.
Interleukin-11 causes alveolar type 2 cell dysfunction and prevents alveolar regeneration
Authors: Ng Benjamin , Huang Kevin Y. , Pua Chee Jian , Viswanathan Sivakumar , Lim Wei-Wen , Kuthubudeen Fathima F. , Liu Yu-Ning , Hii An An , George Benjamin L. , Widjaja Anissa A. , Petretto Enrico , Cook Stuart A. 
Published: October 2024

AbstractIn lung disease, persistence of KRT8-expressing aberrant basaloid cells in the alveolar epithelium is associated with impaired tissue regeneration and pathological tissue remodeling. We analyzed single cell RNA sequencing datasets of human interstitial lung disease and found the profibrotic Interleukin-11 (IL11) cytokine to be highly and specifically expressed in aberrant KRT8+ basaloid cells. IL11 is similarly expressed by KRT8+ alveolar epithelial cells lining fibrotic lesions in a mouse model of interstitial lung disease. Stimulation of alveolar epithelial cells with IL11 causes epithelial-to-mesenchymal transition and promotes a KRT8-high state, which stalls the beneficial differentiation of alveolar type 2 (AT2)-to-AT1 cells. Inhibition of IL11-signaling in AT2 cells in vivo prevents the accumulation of KRT8+ cells, enhances AT1 cell differentiation and blocks fibrogenesis, which is replicated by anti-IL11 therapy. These data show that IL11 inhibits reparative AT2-to-AT1 differentiation in the damaged lung to limit endogenous alveolar regeneration, resulting in fibrotic lung disease.
Cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
Authors: Michael Andrew R. M. , Amaral Bruno C. , Ball Kallie L. , Eiriksson Kristen H. , Schriemer David C. 
Published: October 2024

AbstractCrosslinking mass spectrometry (XL-MS) has the potential to map the interactome of the cell with high resolution and depth of coverage. However, current in vivo XL-MS methods are hampered by crosslinkers that demonstrate low cell permeability and require long reaction times. Consequently, interactome sampling is not high and long incubation times can distort the cell, bringing into question the validity any protein interactions identified by the method. We address these issues with a fast formaldehyde-based fixation method applied prior to the introduction of secondary crosslinkers. Using human A549 cells and a range of reagents, we show that 4% formaldehyde fixation with membrane permeabilization preserves cellular ultrastructure and simultaneously improves reaction conditions for in situ XL-MS. Protein labeling yields can be increased even for nominally membrane-permeable reagents, and surprisingly, high-concentration formaldehyde does not compete with conventional amine-reactive crosslinking reagents. Prefixation with permeabilization uncouples cellular dynamics from crosslinker dynamics, enhancing control over crosslinking yield and permitting the use of any chemical crosslinker.
Grain boundary engineering for efficient and durable electrocatalysis
Authors: Geng Xin , Vega-Paredes Miquel , Wang Zhenyu , Ophus Colin , Lu Pengfei , Ma Yan , Zhang Siyuan , Scheu Christina , Liebscher Christian H. , Gault Baptiste 
Published: October 2024

AbstractGrain boundaries in noble metal catalysts have been identified as critical sites for enhancing catalytic activity in electrochemical reactions such as the oxygen reduction reaction. However, conventional methods to modify grain boundary density often alter particle size, shape, and morphology, obscuring the specific role of grain boundaries in catalytic performance. This study addresses these challenges by employing gold nanoparticle assemblies to control grain boundary density through the manipulation of nanoparticle collision frequency during synthesis. We demonstrate a direct correlation between increased grain boundary density and enhanced two-electron oxygen reduction reaction activity, achieving a significant improvement in both specific and mass activity. Additionally, the gold nanoparticle assemblies with high grain boundary density exhibit remarkable electrochemical stability, attributed to boron segregation at the grain boundaries, which prevents structural degradation. This work provides a promising strategy for optimizing the activity, selectivity, and stability of noble metal catalysts through precise grain boundary engineering.
ARF1 compartments direct cargo flow via maturation into recycling endosomes
Authors: Stockhammer Alexander , Adarska Petia , Natalia Vini , Heuhsen Anja , Klemt Antonia , Bregu Gresy , Harel Shelly , Rodilla-Ramirez Carmen , Spalt Carissa , Özsoy Ece , Leupold Paula , Grindel Alica , Fox Eleanor , Mejedo Joy Orezimena , Zehtabian Amin , Ewers Helge , Puchkov Dmytro , Haucke Volker , Bottanelli Francesca 
Published: October 2024

AbstractCellular membrane homoeostasis is maintained via a tightly regulated membrane and cargo flow between organelles of the endocytic and secretory pathways. Adaptor protein complexes (APs), which are recruited to membranes by the small GTPase ARF1, facilitate cargo selection and incorporation into trafficking intermediates. According to the classical model, small vesicles would facilitate bi-directional long-range transport between the Golgi, endosomes and plasma membrane. Here we revisit the intracellular organization of the vesicular transport machinery using a combination of CRISPR-Cas9 gene editing, live-cell high temporal (fast confocal) or spatial (stimulated emission depletion) microscopy as well as correlative light and electron microscopy. We characterize tubulo-vesicular ARF1 compartments that harbour clathrin and different APs. Our findings reveal two functionally different classes of ARF1 compartments, each decorated by a different combination of APs. Perinuclear ARF1 compartments facilitate Golgi export of secretory cargo, while peripheral ARF1 compartments are involved in endocytic recycling downstream of early endosomes. Contrary to the classical model of long-range vesicle shuttling, we observe that ARF1 compartments shed ARF1 and mature into recycling endosomes. This maturation process is impaired in the absence of AP-1 and results in trafficking defects. Collectively, these data highlight a crucial role for ARF1 compartments in post-Golgi sorting.
GDEMM2024: Global Digital Elevation Merged Model 2024 for surface, bedrock, ice thickness, and land-type masks
Authors: Ince E. Sinem , Abrykosov Oleh , Förste Christoph 
Published: October 2024

AbstractVarious research topics in geosciences such as gravity modelling, terrain correction and ocean circulation, require high resolution and accuracy global elevations for land topography, bathymetry, and ice thickness that refer to a consistent vertical datum. Unfortunately, most of the existing DEMs do not provide such solutions for Earth relief layers with the same resolution globally. To overcome this deficiency, we merged various DEMs published in the recent years and compiled an up-to-date global solution. We provide 30 arcsecond grid suite for relief layers and land-type masks which have been substantially improved w.r.t. the grids in literature. The quality of the merged surface elevation is assessed against the GNSS heights at about globally distributed 22000 stations. The merged surface model shows a reduction in standard deviation of a factor of three compared to other commonly used DEMs. Other evaluations are performed over land-ice and oceans which supports the advancement of GDEMM2024. The improvements are due to the accuracy and coverage of the original input data, updated land-type masks and merging methodology.
Patterns of intersectional tumor volumes in T2-weighted MRI and [18F]FET PET in adult glioma: a prospective, observational study
Authors: Weller Jonathan , Unterrainer Marcus , Sonderer Markéta , Katzendobler Sophie , Holzgreve Adrien , Biczok Annamaria , Harter Patrick N. , Tonn Joerg-Christian , Albert Nathalie L. , Suchorska Bogdana 
Published: October 2024

AbstractBrain tumor volumes as assessed by magnetic resonance imaging (MRI) do not always spatially overlap with biological tumor volumes (BTV) measured by [18F]Fluoroethyltyrosine positron emission tomography ([18F]FET PET). We prospectively investigated volumetric patterns based on the extent of tumor volume overlap between the two modalities. Eighty-six patients with newly diagnosed glioma who had undergone MRI and [18F]FET PET between 2007 and 2009 were included in this prospective study and (re-)classified according to CNS WHO 2021 (Classification of Tumors of the Central Nervous System by the World Health Organization). Four different patterns of volume overlap were defined mathematically according to the extent of overlap between MRI-based T2 tumor volume (non-enhancing tumor volume, nCEV) and BTVs. Progression-free (PFS) and overall survival (OS) were determined. Seventy patients were diagnosed with isocitrate dehydrogenase wildtype (IDHwt) glioblastoma and 16 with IDH-mutant glioma, respectively. The most common pattern was characterized by a larger non-contrast-enhancing tumor volume (nCEV) that enclosed all or most of the BTV and was observed in 46 patients (54%) (pattern 1). This pattern was more frequent in IDH-mutant gliomas than in IDH-wildtype glioblastoma (81% versus 47%, p = 0.02). In multivariate analyses, pattern 1 was associated with prolonged PFS (HR 0.59; 95 CI 0.34-1.0; p = 0.05), but not OS (HR 0.66; 95 CI 0.4–1.08; p = 0.1). For OS, presence of an IDH mutation (p = 0.05) and lower age (p = 0.03) were associated with prolonged OS. The spatial relation between nCEV and BTV varies within and between glioma entities. Most frequently, a larger nCEV encases the BTV. Some patients show spatially dissociated nCEVs and BTVs. Not accounting for this phenomenon in surgery or radiotherapy planning might lead to undertreatment.
Mechanism of enhanced salt tolerance in Saccharomyces cerevisiae by CRZ1 overexpression
Authors: Zuo Furong , Wu Yajing , Sun Yanqiu , Xie Caiyun , Tang Yueqin 
Published: October 2024

AbstractAchieving high-gravity fermentation in the industrial production of fuel ethanol, and enhancing the fermentation efficiency of high-salt raw materials, such as waste molasses, can significantly reduce wastewater output and process costs. Therefore, the development of hyperosmotic-tolerant industrial Saccharomyces cerevisiae strains, capable of resisting high-salt stress, offers both environmental and economic benefits. Our previous study highlighted the potential of CRZ1 overexpression as a strategy to improve the yeast strain’s resistance to high-salt stress, however, the underlying molecular mechanisms remain unexplored. The fermentation capabilities of the CRZ1-overexpressing strain, KCR3, and its parental strain, KF7, were evaluated under condition of 1.25 M NaCl at 35 °C. Compared to KF7, KCR3 showed an 81% increase in glucose consumption (129.25 ± 0.83 g/L) and a 105% increase in ethanol production (47.59 ± 0.93 g/L), with a yield of 0.37 g/g. Comparative transcriptomic analysis showed that under high-salt stress, KCR3 exhibited significantly upregulated expression of genes associated with ion transport, stress response, gluconeogenesis, and the utilization of alternative carbon sources, while genes related to glycolysis and the biosynthesis of ribosomes, amino acids, and fatty acids were notably downregulated compared to KF7. Crz1 likely expands its influence by regulating the expression of numerous transcription factors, thereby impacting genes involved in multiple aspects of cellular function. The study revealed the regulatory mechanism of Crz1 under high-salt stress, thereby providing guidance for the construction of salt-tolerant strains.
Antarctic sea ice surface temperature bias in atmospheric reanalyses induced by the combined effects of sea ice and clouds
Authors: Wang Zhaohui , Fraser Alexander D. , Reid Phillip , O’Farrell Siobhan , Coleman Richard 
Published: October 2024

AbstractSea-ice surface temperature from atmospheric reanalysis has been used as an indicator of ice melt and climate change. However, its performance in atmospheric reanalyses is not fully understood in Antarctica. Here, we quantified biases in six widely-used reanalyses using satellite observations, and found strong and persistent warm biases in most reanalyses examined. Further analysis of the biases revealed two main culprits: incorrect cloud properties, and inappropriate sea-ice representation in the reanalysis products. We found that overestimated cloud simulation can contribute more than 4 K warm bias, with ERA5 exhibiting the largest warm bias. Even in reanalysis with smaller biases, this accuracy is achieved through a compensatory relationship between relatively lower cloud fraction bias and overestimated sea ice insulation effect. A dynamic downscaling simulation shows that differences in sea-ice representation can contribute a 2.3 K warm bias. The representation of ice concentration is the primary driver of the spatial distribution of biases by modulating the coupling between sea ice and clouds, as well as surface heat conduction. The lack of a snow layer in all reanalyses examined also has an impact on biases.
Lower Blood Loss with Bicruciate-Retaining Total Knee Arthroplasty Compared with Bicruciate-Stabilized Total Knee Arthroplasty
Authors: Kozu Takashi , Iriuchishima Takanori , Ryu Keinosuke , Nakanishi Kazuyoshi 
Published: October 2024

AbstractTotal knee arthroplasty (TKA) is a common surgical procedure to treat end-stage knee osteoarthritis. This study compared blood loss volume and other clinical outcomes between bicruciate-retaining (BCR) and bicruciate-stabilized (BCS) TKA. Ninety-seven participants who underwent unilateral TKA were enrolled. The BCS-TKA and BCR-TKA groups comprised 78 and 19 participants, respectively. Blood loss was calculated using preoperative and postoperative hematocrit values, height, weight, and sex. Measurements were taken immediately after surgery, on days 1 and 7, and total blood loss was calculated up to day 7. Operation time, range of motion at 7 and 14 days postoperatively, blood transfusion requirement, and postoperative complications such as infection, hematoma, and deep vein thrombosis were compared between the groups. Blood loss was comparable between groups at all time points (125.7 vs. 105.4 mL, 57.9 vs. 43.5 mL, and 68.2 vs. 41.7 mL for immediate, day 1, and day 7 postoperatively, respectively; all p > 0.05) except for total blood loss, which was significantly lower in the BCR group compared with the BCS group (190.7 vs. 251.1 mL; p < 0.05). The BCR group also had a longer operation time than the BCS group (131.2 vs. 112.4 minutes; p < 0.05). No other significant differences were observed in other outcomes. Total blood loss up to 7 days postoperatively was significantly lower in the BCR group than in the BCS group despite a longer operation time. This may be attributed to the reduced bone resection and greater soft tissue preservation, including the anterior cruciate and posterior cruciate ligaments, in the BCR technique.
Intradural Repair of Temporal Bone Encephalocele and Cerebrospinal Fluid Leak: Results from a Single Institution
Authors: Ellsperman Susan E. , D'Agostino Anna K. , Olszewski Adam M. , Peng Kevin A. , Slattery William H. , Lekovic Gregory P. 
Published: October 2024

Abstract
Background Lateral temporal bone encephaloceles incidence is increasing with obesity rates. Middle fossa (MF) craniotomy, transmastoid (TM), or combined MF + TM access can be used for repair.
Methods Retrospective review of MF or MF + TM repair with an intradural graft. Sex, age, and body mass index (BMI) were collected. Pre/postoperative audiometric results were included. Postoperative complications were reported.
Results A total of 49 patients (50 repairs) were included. In addition, 74% were women (p < 0.05). Ten patients had a history of chronic otitis media and surgery. Average BMI was 35.8, and average age was 59. Furthermore, 54% had multiple skull base defects; 18 (36%) patients had a MF approach. In total, 32 (64%) patients had a MF + TM approach for repair; 13 (40.1%) of these patients had a concurrent tympanoplasty. Hearing improved for 74%. Air conduction pure-tone average improved by an average of 5 dB (p 0.27). No differences in hearing outcomes were observed between the MF and MF + TM groups. Two patients (6%) had hearing decline. Eight complications were reported (1 (2%) skin infection, 4 (8%) recurrent/persistent cerebrospinal fluid [CSF] leaks requiring lumbar drain or shunt, and 3 (6%) episodes of aphasia or mental status change). Age >65 years was not associated with risk of postoperative complication.
Conclusion Intradural repair of encephalocele and CSF leak is a safe and effective surgical approach. Intradural reinforcement along the entire MF floor is beneficial for multiple areas of dehiscence and thin dura. Complication rates including recurrent/persistent CSF leak and aphasia related to temporal lobe retraction were similar to previously published reports and not associated with older patient age. Hearing was stable or improved in 94% with no difference noted between MF and MF + TM repair.