bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stimulated left DLPFC-nucleus accumbens functional connectivity predicts the anti-depression and anti-anxiety effects of rTMS for depression
Authors: Du Lian , Liu Huan , Du Wanyi , Chao Fenglei , Zhang Lei , Wang Kejian , Huang Chunxia , Gao Yuan , Tang Yong 
Published: November 2017

AbstractNot all depression patients effectively respond to repeated transcranial magnetic stimulation (rTMS). We tested whether the intrinsic functional connectivity (FC) strength between the stimulated left dorsolateral prefrontal cortex (DLPFC) and left nucleus accumbens (NAcc) might predict effects of rTMS. Twenty-two medication-naïve depression patients received rTMS on left DLPFC for 2 weeks and underwent baseline functional magnetic resonance imaging (fMRI). We compared the amplitude of the low-frequency fluctuation (ALFF) and regional homogeneity (ReHo) in the stimulated target (the cortex region directly stimulated by rTMS) located in the left DLPFC, and the left NAcc, as well as the intrinsic FC of the DLPFC–NAcc between early improvers and non-improvers. We evaluated the association between the baseline brain imaging features (ALFF, ReHo, and FC) and improvements in depression and anxiety symptoms. We found that the pretreatment ALFF and ReHo in the stimulated DLPFC and left NAcc did not significantly differ between the subgroups. The early improvers displayed increased negative FC strength between the stimulated DLPFC and left NAcc with respect to non-improvers. The stimulated DLPFC–NAcc FC strength negatively correlated with improved depressive and anxious symptoms. This study is the first to demonstrate that the resting-state FC of the stimulated DLPFC–NAcc, rather than regional brain activity or local synchronization in the stimulated target, might predict the anti-depression and anti-anxiety effects of rTMS for depression.
ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence
Authors: Zhou Li , Xiong Jing , Ruan Chun-Sheng , Ruan Ye , Liu Dennis , Bao Jian-Jun , Zhou Xin-Fu 
Published: November 2017

AbstractAlcohol dependence is a worldwide problem with a great social and economic burden in many countries. A number of studies have suggested that BDNF (mature BDNF) and its precursor (proBDNF) play important roles in the alcohol dependence. However, what roles of the mBDNF/proBDNF pathways play during the pathological process of alcohol dependence are not clearly understood. In our clinical study, peripheral blood was sampled from 30 male patients with alcohol dependence and 50 healthy males (as control). The protein levels of proBDNF, p75NTR, sortilin, mBDNF, TrkB and mRNA levels ofBDNF,p75NTR,sortilin, andTrkBwere detected in the peripheral blood in our study. We found that the protein levels of proBDNF and p75NTR were increased, but not the sortilin protein level; while the TrkB protein level was decreased in the alcohol dependence patients compared with healthy controls. Moreover, the mRNA levels ofp75NTRandsortilinfrom the lymphocytes were slightly increased; whileBDNFandTrkBwere significantly decreased. The ELISA results of mBDNF and TrkB were declined in the alcohol dependence group. The levels of mBDNF and TrkB were negatively correlated with the average amount of daily ethanol consumption, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the average amount of ethanol consumption per day. The ratio of proBDNF to mBDNF was altered in alcohol dependence patients. The balance between the proBDNF/p75NTR and mBDNF/TrkB signalling pathways appeared dysregulated in alcohol dependence. Our results suggested that both pathways may participate in the complex processes of alcohol dependence.
Resistance to cancer immunotherapy mediated by apoptosis of tumor-infiltrating lymphocytes
Authors: Zhu Jingjing , Powis de Tenbossche Céline G. , Cané Stefania , Colau Didier , van Baren Nicolas , Lurquin Christophe , Schmitt-Verhulst Anne-Marie , Liljeström Peter , Uyttenhove Catherine , Van den Eynde Benoit J. 
Published: November 2017

AbstractDespite impressive clinical success, cancer immunotherapy based on immune checkpoint blockade remains ineffective in many patients due to tumoral resistance. Here we use the autochthonous TiRP melanoma model, which recapitulates the tumoral resistance signature observed in human melanomas. TiRP tumors resist immunotherapy based on checkpoint blockade, cancer vaccines or adoptive T-cell therapy. TiRP tumors recruit and activate tumor-specific CD8+T cells, but these cells then undergo apoptosis. This does not occur with isogenic transplanted tumors, which are rejected after adoptive T-cell therapy. Apoptosis of tumor-infiltrating lymphocytes can be prevented by interrupting the Fas/Fas-ligand axis, and is triggered by polymorphonuclear-myeloid-derived suppressor cells, which express high levels of Fas-ligand and are enriched in TiRP tumors. Blocking Fas-ligand increases the anti-tumor efficacy of adoptive T-cell therapy in TiRP tumors, and increases the efficacy of checkpoint blockade in transplanted tumors. Therefore, tumor-infiltrating lymphocytes apoptosis is a relevant mechanism of immunotherapy resistance, which could be blocked by interfering with the Fas/Fas-ligand pathway.
Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer’s disease
Authors: Mann Aman P. , Scodeller Pablo , Hussain Sazid , Braun Gary B. , Mölder Tarmo , Toome Kadri , Ambasudhan Rajesh , Teesalu Tambet , Lipton Stuart A. , Ruoslahti Erkki 
Published: November 2017

AbstractCerebrovascular changes occur in Alzheimer’s disease (AD). Using in vivo phage display, we searched for molecular markers of the neurovascular unit, including endothelial cells and astrocytes, in mouse models of AD. We identified a cyclic peptide, CDAGRKQKC (DAG), that accumulates in the hippocampus of hAPP-J20 mice at different ages. Intravenously injected DAG peptide homes to neurovascular unit endothelial cells and to reactive astrocytes in mouse models of AD. We identified connective tissue growth factor (CTGF), a matricellular protein that is highly expressed in the brain of individuals with AD and in mouse models, as the target of the DAG peptide. We also showed that exogenously delivered DAG homes to the brain in mouse models of glioblastoma, traumatic brain injury, and Parkinson’s disease. DAG may potentially be used as a tool to enhance delivery of therapeutics and imaging agents to sites of vascular changes and astrogliosis in diseases associated with neuroinflammation.
Symmetry control of nanorod superlattice driven by a governing force
Authors: Liang Yujia , Xie Yong , Chen Dongxue , Guo Chuanfei , Hou Shuai , Wen Tao , Yang Fengyou , Deng Ke , Wu Xiaochun , Smalyukh Ivan I. , Liu Qian 
Published: November 2017

AbstractNanoparticle self-assembly promises scalable fabrication of composite materials with unique properties, but symmetry control of assembled structures remains a challenge. By introducing a governing force in the assembly process, we develop a strategy to control assembly symmetry. As a demonstration, we realize the tetragonal superlattice of octagonal gold nanorods, breaking through the only hexagonal symmetry of the superlattice so far. Surprisingly, such sparse tetragonal superstructure exhibits much higher thermostability than its close-packed hexagonal counterpart. Multiscale modeling reveals that the governing force arises from hierarchical molecular and colloidal interactions. This force dominates the interactions involved in the assembly process and determines the superlattice symmetry, leading to the tetragonal superlattice that becomes energetically favorable over its hexagonal counterpart. This strategy might be instructive for designing assembly of various nanoparticles and may open up a new avenue for realizing diverse assembly structures with pre-engineered properties.
Phospholipid flippase ATP11C is endocytosed and downregulated following Ca2+-mediated protein kinase C activation
Authors: Takatsu Hiroyuki , Takayama Masahiro , Naito Tomoki , Takada Naoto , Tsumagari Kazuya , Ishihama Yasushi , Nakayama Kazuhisa , Shin Hye-Won 
Published: November 2017

AbstractWe and others showed that ATP11A and ATP11C, members of the P4-ATPase family, translocate phosphatidylserine (PS) and phosphatidylethanolamine from the exoplasmic to the cytoplasmic leaflets at the plasma membrane. PS exposure on the outer leaflet of the plasma membrane in activated platelets, erythrocytes, and apoptotic cells was proposed to require the inhibition of PS-flippases, as well as activation of scramblases. Although ATP11A and ATP11C are cleaved by caspases in apoptotic cells, it remains unclear how PS-flippase activity is regulated in non-apoptotic cells. Here we report that the PS-flippase ATP11C, but not ATP11A, is sequestered from the plasma membrane via clathrin-mediated endocytosis upon Ca2+-mediated PKC activation. Importantly, we show that a characteristic di-leucine motif (SVRPLL) in the C-terminal cytoplasmic region of ATP11C becomes functional upon PKC activation. Moreover endocytosis of ATP11C is induced by Ca2+-signaling via Gq-coupled receptors. Our data provide the first evidence for signal-dependent regulation of mammalian P4-ATPase.
A thermostable Cas9 with increased lifetime in human plasma
Authors: Harrington Lucas B. , Paez-Espino David , Staahl Brett T. , Chen Janice S. , Ma Enbo , Kyrpides Nikos C. , Doudna Jennifer A. 
Published: November 2017

AbstractCRISPR-Cas9 is a powerful technology that has enabled genome editing in a wide range of species. However, the currently developed Cas9 homologs all originate from mesophilic bacteria, making them susceptible to degradation and unsuitable for applications requiring cleavage at elevated temperatures. Here, we show that the Cas9 protein from the thermophilic bacterium Geobacillus stearothermophilus (GeoCas9) catalyzes RNA-guided DNA cleavage at elevated temperatures. GeoCas9 is active at temperatures up to 70 °C, compared to 45 °C for Streptococcus pyogenes Cas9 (SpyCas9), which expands the temperature range for CRISPR-Cas9 applications. We also found that GeoCas9 is an effective tool for editing mammalian genomes when delivered as a ribonucleoprotein (RNP) complex. Together with an increased lifetime in human plasma, the thermostable GeoCas9 provides the foundation for improved RNP delivery in vivo and expands the temperature range of CRISPR-Cas9.
Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology
Authors: Hammam Kahina , Saez-Ayala Magali , Rebuffet Etienne , Gros Laurent , Lopez Sophie , Hajem Berengere , Humbert Martine , Baudelet Emilie , Audebert Stephane , Betzi Stephane , Lugari Adrien , Combes Sebastien , Letard Sebastien , Casteran Nathalie , Mansfield Colin , Moussy Alain , De Sepulveda Paulo , Morelli Xavier , Dubreuil Patrice 
Published: November 2017

AbstractMasitinib, a highly selective protein kinase inhibitor, can sensitise gemcitabine-refractory cancer cell lines when used in combination with gemcitabine. Here we report a reverse proteomic approach that identifies the target responsible for this sensitisation: the deoxycytidine kinase (dCK). Masitinib, as well as other protein kinase inhibitors, such as imatinib, interact with dCK and provoke an unforeseen conformational-dependent activation of this nucleoside kinase, modulating phosphorylation of nucleoside analogue drugs. This phenomenon leads to an increase of prodrug phosphorylation of most of the chemotherapeutic drugs activated by this nucleoside kinase. The unforeseen dual activity of protein kinase inhibition/nucleoside kinase activation could be of great therapeutic benefit, through either reducing toxicity of therapeutic agents by maintaining effectiveness at lower doses or by counteracting drug resistance initiated via down modulation of dCK target.
Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo
Authors: Bischof Helmut , Rehberg Markus , Stryeck Sarah , Artinger Katharina , Eroglu Emrah , Waldeck-Weiermair Markus , Gottschalk Benjamin , Rost Rene , Deak Andras T. , Niedrist Tobias , Vujic Nemanja , Lindermuth Hanna , Prassl Ruth , Pelzmann Brigitte , Groschner Klaus , Kratky Dagmar , Eller Kathrin , Rosenkranz Alexander R. , Madl Tobias , Plesnila Nikolaus , Graier Wolfgang F. , Malli Roland 
Published: November 2017

AbstractChanges in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K+ changes is severely limited by the lack of practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K+ dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K+ levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K+ signals. Hitherto unknown Ca2+-triggered, organelle-specific K+ changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K+ fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K+ assays and open new avenues for live-cell K+ imaging.
Purcell-enhanced quantum yield from carbon nanotube excitons coupled to plasmonic nanocavities
Authors: Luo Yue , Ahmadi Ehsaneh D. , Shayan Kamran , Ma Yichen , Mistry Kevin S. , Zhang Changjian , Hone James , Blackburn Jeffrey L. , Strauf Stefan 
Published: November 2017

AbstractSingle-walled carbon nanotubes (SWCNTs) are promising absorbers and emitters to enable novel photonic applications and devices but are also known to suffer from low optical quantum yields. Here we demonstrate SWCNT excitons coupled to plasmonic nanocavity arrays reaching deeply into the Purcell regime with Purcell factors (FP) up to FP = 180 (average FP = 57), Purcell-enhanced quantum yields of 62% (average 42%), and a photon emission rate of 15 MHz into the first lens. The cavity coupling is quasi-deterministic since the photophysical properties of every SWCNT are enhanced by at least one order of magnitude. Furthermore, the measured ultra-narrow exciton linewidth (18 μeV) reaches the radiative lifetime limit, which is promising towards generation of transform-limited single photons. To demonstrate utility beyond quantum light sources we show that nanocavity-coupled SWCNTs perform as single-molecule thermometers detecting plasmonically induced heat at cryogenic temperatures in a unique interplay of excitons, phonons, and plasmons at the nanoscale.
Cooperative polariton dynamics in feedback-coupled cavities
Authors: Yao Bimu , Gui Y. S. , Rao J. W. , Kaur S. , Chen X. S. , Lu W. , Xiao Y. , Guo H. , Marzlin K. -P. , Hu C. -M. 
Published: November 2017

AbstractThe emerging field of cavity spintronics utilizes the cavity magnon polariton (CMP) induced by magnon Rabi oscillations. In contrast to a single-spin quantum system, such a cooperative spin dynamics in the linear regime is governed by the classical physics of harmonic oscillators. It makes the magnon Rabi frequency independent of the photon Fock state occupation, and thereby restricts the quantum application of CMP. Here we show that a feedback cavity architecture breaks the harmonic-oscillator restriction. By increasing the feedback photon number, we observe an increase in the Rabi frequency, accompanied with the evolution of CMP to a cavity magnon triplet and a cavity magnon quintuplet. We present a theory that explains these features. Our results reveal the physics of cooperative polariton dynamics in feedback-coupled cavities, and open up new avenues for exploiting the light–matter interactions.
Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective
Authors: Angioletti-Uberti Stefano 
Published: November 2017

AbstractFunctionalised nanoparticles for biomedical applications represents an incredibly exciting and rapidly growing field of research. Considering the complexity of the nano–bio interface, an important question is to what extent can theory and simulations be used to study these systems in a realistic, meaningful way. In this review, we will argue for a positive answer to this question. Approaching the issue from a “Soft Matter” perspective, we will consider those properties of functionalised nanoparticles that can be captured within a classical description. We will thus not concentrate on optical and electronic properties, but rather on the way nanoparticles’ interactions with the biological environment can be tuned by functionalising their surface and exploited in different contexts relevant to applications. In particular, we wish to provide a critical overview of theoretical and computational coarse-grained models, developed to describe these interactions and present to the readers some of the latest results in this fascinating area of research.
Involvement of galanin and galanin receptor 1 in nociceptive modulation in the central nucleus of amygdala in normal and neuropathic rats
Authors: Li Shi-Yang , Huo Mei-Ling , Wu Xu-Yang , Huang Yu-Qing , Wang Lei , Zhang Xin , Jiang Yan-Mei , Zhang Meng-Lin , Wang Lin-Lin , Yu Long-Chuan 
Published: November 2017

AbstractThe present study was performed to explore the role of galanin and galanin receptor 1 (GalR 1) in nociceptive modulation in the central nucleus of amygdala (CeA) in normal rats and rats with neuropathy, and the involvement of GalR 1 and PKC was also investigated. The hindpaw withdrawal latencies (HWLs) to thermal and mechanical stimulations were increased in a dose-dependent manner after intra-CeA injection of galanin in both normal rats and rats with neuropathy. The increased HWLs were significantly attenuated by intra-CeA injection of galanin receptor antagonist M40, indicating an involvement of galanin receptor in nociceptive modulation in CeA. Furthermore, intra-CeA administration of the GalR 1 agonist M 617 induced increases in HWLs in normal rats, suggesting that GalR 1 may be involved in galanin-induce antinociception in CeA. Additionally, intra-CeA injection of the PKC inhibitor inhibited galanin-induced antinociception, showing an involvement of PKC in galanin-induced antinociception in CeA of normal rats. Moreover, there was a significant increase in GalR1 content in CeA in rats with neuropathy than that in normal rats. These results illustrated that galanin induced antinociception in CeA in normal rats and rats with neuropathy, and there is an up-regulation of GalR1 expression in rats with neuropathy.
Clinical Potential of an Enzyme-based Novel Therapy for Cocaine Overdose
Authors: Zhang Ting , Zheng Xirong , Zhou Ziyuan , Chen Xiabin , Jin Zhenyu , Deng Jing , Zhan Chang-Guo , Zheng Fang 
Published: November 2017

AbstractIt is a grand challenge to develop a truly effective medication for treatment of cocaine overdose. The current available, practical emergence treatment for cocaine overdose includes administration of a benzodiazepine anticonvulsant agent (e.g. diazepam) and/or physical cooling with an aim to relieve the symptoms. The inherent difficulties of antagonizing physiological effects of drugs in the central nervous system have led to exploring protein-based pharmacokinetic approaches using biologics like vaccines, monoclonal antibodies, and enzymes. However, none of the pharmacokinetic agents has demonstrated convincing preclinical evidence of clinical potential for drug overdose treatment without a question mark on the timing used in the animal models. Here we report the use of animal models, including locomotor activity, protection, and rescue experiments in rats, of drug toxicity treatment with clinically relevant timing for the first time. It has been demonstrated that an efficient cocaine-metabolizing enzyme developed in our previous studies can rapidly reverse the cocaine toxicity whenever the enzyme is given to a living rat, demonstrating promising clinical potential of an enzyme-based novel therapy for cocaine overdose as a successful example in comparison with the commonly used diazepam.
A viscous quantum hydrodynamics model based on dynamic density functional theory
Authors: Diaw Abdourahmane , Murillo Michael S. 
Published: November 2017

AbstractDynamic density functional theory (DDFT) is emerging as a useful theoretical technique for modeling the dynamics of correlated systems. We extend DDFT to quantum systems for application to dense plasmas through a quantum hydrodynamics (QHD) approach. The DDFT-based QHD approach includes correlations in the the equation of state self-consistently, satisfies sum rules and includes irreversibility arising from collisions. While QHD can be used generally to model non-equilibrium, heterogeneous plasmas, we employ the DDFT-QHD framework to generate a model for the electronic dynamic structure factor, which offers an avenue for measuring hydrodynamic properties, such as transport coefficients via x-ray Thomson scattering.
Spatially Resolved Thermometry of Resistive Memory Devices
Authors: Yalon Eilam , Deshmukh Sanchit , Muñoz Rojo Miguel , Lian Feifei , Neumann Christopher M. , Xiong Feng , Pop Eric 
Published: November 2017

AbstractThe operation of resistive and phase-change memory (RRAM and PCM) is controlled by highly localized self-heating effects, yet detailed studies of their temperature are rare due to challenges of nanoscale thermometry. Here we show that the combination of Raman thermometry and scanning thermal microscopy (SThM) can enable such measurements with high spatial resolution. We report temperature-dependent Raman spectra of HfO2, TiO2 and Ge2Sb2Te5 (GST) films, and demonstrate direct measurements of temperature profiles in lateral PCM devices. Our measurements reveal that electrical and thermal interfaces dominate the operation of such devices, uncovering a thermal boundary resistance of 28 ± 8 m2K/GW at GST-SiO2 interfaces and an effective thermopower 350 ± 50 µV/K at GST-Pt interfaces. We also discuss possible pathways to apply Raman thermometry and SThM techniques to nanoscale and vertical resistive memory devices.
On Economic Complexity and the Fitness of Nations
Authors: Morrison Greg , Buldyrev Sergey V. , Imbruno Michele , Doria Arrieta Omar Alonso , Rungi Armando , Riccaboni Massimo , Pammolli Fabio 
Published: November 2017

AbstractComplex economic systems can often be described by a network, with nodes representing economic entities and edges their interdependencies, while network centrality is often a good indicator of importance. Recent publications have implemented a nonlinear iterative Fitness-Complexity (FC) algorithm to measure centrality in a bipartite trade network, which aims to represent the ‘Fitness’ of national economies as well as the ‘Complexity’ of the products being traded. In this paper, we discuss this methodological approach and conclude that further work is needed to identify stable and reliable measures of fitness and complexity. We provide theoretical and numerical evidence for the intrinsic instability in the nonlinear definition of the FC algorithm. We perform an in-depth evaluation of the algorithm’s rankings in two real world networks at the country level: the global trade network, and the patent network in different technological domains. In both networks, we find evidence of the instabilities predicted theoretically, and show that ‘complex’ products or patents tend often to be those that countries rarely produce, rather than those that are intrinsically more difficult to produce.