bluuebunny/crossref_metadata_2025_split huggingface dataset:
Particles of different sizes and shapes induce neutrophil necroptosis followed by the release of neutrophil extracellular trap-like chromatin
Authors: Desai Jyaysi , Foresto-Neto Orestes , Honarpisheh Mohsen , Steiger Stefanie , Nakazawa Daigo , Popper Bastian , Buhl Eva Miriam , Boor Peter , Mulay Shrikant R. , Anders Hans-Joachim 
Published: October 2017

AbstractThe human body is exposed to a wide range of particles of industrial, environmental or internal origin such as asbestos, alum, silica or crystals of urate, calcium phosphate, calcium oxalate, cystine or cholesterol. Phagocytic clearance of such particles involves neutrophils and macrophages. Here we report that neutrophils encountering such particles of diverse sizes and shapes undergo necrotic cell death, a process associated with the formation of neutrophil extracellular trap (NET)-like extracellular DNA. In human neutrophils receptor-interacting protein kinase (RIPK)-1 inhibition with necrostatin-1s or mixed lineage kinase domain-like (MLKL) inhibition with necrosulfonamide abrogated cell death and associated-neutrophil extracellular DNA release induced by all of the aforementioned particles. Similar results were obtained with Mlkl-deficient mice neutrophils for all particles in vitro. Furthermore, Mlkl-deficient mice lacked tophus formation upon injection of MSU crystals into subcutaneous air pouches. These findings imply that nano- or microparticle-induced neutrophil extracellular DNA release is the consequence of neutrophil necroptosis, a regulated form of cell necrosis defined by RIPK1-RIPK3-MLKL signaling. Interestingly, this finding was consistent across different particle sizes and shapes. The RIPK1-RIPK3-MLKL signaling pathway may represent a potential therapeutic target in nano- or microparticle-related diseases (crystallopathies).
Atomic-scale analysis of cation ordering in reduced calcium titanate
Authors: Li Luying , Hu Xiaokang , Jiang Fan , Jing Wenkui , Guo Cong , Jia Shuangfeng , Gao Yihua , Wang Jianbo 
Published: October 2017

AbstractThe phenomenon of cation ordering is closely related to certain physical properties of complex oxides, which necessitates the search of underlying structure-property relationship at atomic resolution. Here we study the superlattices within reduced calcium titanate single crystal micro-pillars, which are unexpected from the originally proposed atomic model. Bright and dark contrasts at alternating Ti double layers perpendicular to b axis are clearly observed, but show no signs in corresponding image simulations based on the proposed atomic model. The multi-dimensional chemical analyses at atomic resolution reveal periodic lower Ti concentrations at alternating Ti double layers perpendicular to b axis. The following in-situ heating experiment shows no phase transition at the reported Tc and temperature independence of the superlattices. The dimerization of the Ti-Ti bonds at neighboring double rutile-type chains within Ti puckered sheets are directly observed, which is found to be not disturbed by the cation ordering at alternating Ti double layers. The characterization of cation ordering of complex oxides from chemical and structural point of view at atomic resolution, and its reaction to temperature variations are important for further understanding their basic physical properties and exploiting potential applications.
Self-template synthesis of biomass-derived 3D hierarchical N-doped porous carbon for simultaneous determination of dihydroxybenzene isomers
Authors: Chen Dejian , Zhou Haifeng , Li Hao , Chen Jie , Li Shunxing , Zheng Fengying 
Published: October 2017

AbstractNitrogen doped hierarchical porous carbon materials (HPCs) was achieved by the successful carbonization, using pig lung as biomass precursor. Three-dimensional HPCs constituted with sheets and lines were synergistically inherited from original pig lung. Such structure provided a large specific surface area (958.5 g−1 m2) and rich porous, effectively supported a large number of electro-active species, and greatly enhanced the mass and electron transfer. High graphitization degree of HPCs resulted in good electrical conductivity. Furthermore, the different electronegativity between nitrogen and carbon atoms in HPCs could affect the electron cloud distribution, polarity and then the electrochemical oxidation kinetics of dihydroxybenzene isomers. Based on these characteristics of HPCs, the electrochemical sensor for dihydroxybenzene isomers exhibited high sensitivity, excellent specificity and stability. Quantitative analysis assays by differential pulse voltammetry (DPV) technology showed that the sensor has wide linear ranges (0.5–320, 0.5–340 and 1–360 μmol L−1) and low detection limits (0.078, 0.057 and 0.371 μmol L−1) for the catechol, resorcinol and hydroquinone, respectively. This proposed method was successfully applied for simultaneous detection of dihydroxybenzene isomers in river water.
The influence of experience on contest assessment strategies
Authors: Camerlink Irene , Turner Simon P. , Farish Marianne , Arnott Gareth 
Published: October 2017

AbstractAnimal contest behaviour has been widely studied, yet major knowledge gaps remain concerning the information-gathering and decision-making processes used during encounters. The mutual assessment strategy, where the individual assesses its own fighting ability (Resource Holding Potential, RHP) and compares it to that of its opponent, is least understood. We hypothesise that individuals need experience of agonistic encounters to become proficient at mutual assessment. Pigs (Sus scrofa, n = 316) were contested twice. In between contests, animals did or did not (control) receive intense fighting experience. A substantial proportion of the contests reached an outcome with a clear winner without fighting. Non-escalation was highest in RHP asymmetric dyads of the second contest, irrespective of experience. In contest 1 (no experience) and in contest 2 for the experienced animals, costs increased with loser RHP and where unaffected by winner RHP, suggesting a self-assessment strategy. In contest 2 control dyads, which only had experience of one prior contest, a negative relation between winner RHP and costs suggested mutual assessment during the pre-escalation phase but not during escalated aggression. This reveals that a brief and relatively mild experience can be beneficial in the development of mutual assessment whereas profound experience may result in adoption of a self-assessment strategy.
Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
Authors: Koh Hyeon-Woo , Hur Moonsuk , Kang Myung-Suk , Ku Youn-Bong , Ghai Rohit , Park Soo-Je 
Published: October 2017

AbstractArsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize arsenite, and investigated its physiological and genomic traits. Its cells were rod-shaped and Gram-negative, and formed small beige-pigmented colonies. 16S rRNA-based phylogenetic analysis indicated that the strain belongs to the genusHerminiimonasand is closely related toHerminiimonas glacieiUMB49T(98.7% of 16S rRNA gene sequence similarity),Herminiimonas arsenicoxydansULPAs1T(98.4%), andHerminiimonas saxobsidensNS11T(98.4%). Under chemolithoheterotrophic conditions, the strain utilized some organic acids and amino acids as carbon and/or nitrogen sources but not electron sources. Further, the strain grew as a sulfur oxidizer in a complex medium (trypticase soy agar). Unexpectedly, most carbohydrates failed to support its growth as sole carbon sources. Genome sequencing supported these observations, and very few ABC transporters capable of oligo/monosaccharide uptake were identified in the AS8 genome. The genome harbored genes required for the colonization, flagella biosynthesis, urea degradation, and heavy metal and antibiotic resistance. Based on these polyphasic and genomic analyses, we propose that the strain AS8 be namedHerminiimonas arsenitoxidans.
Reduced Graphene Oxide-Gold Nanoparticle Nanoframework as a Highly Selective Separation Material for Aflatoxins
Authors: Guo Wenbo , Wu Lidong , Fan Kai , Nie Dongxia , He Weijing , Yang Junhua , Zhao Zhihui , Han Zheng 
Published: October 2017

AbstractGraphene-based materials have been studied in many applications, owing to the excellent electrical, mechanical, and thermal properties of graphene. In the current study, an environmentally friendly approach to the preparation of a reduced graphene oxide-gold nanoparticle (rGO-AuNP) nanocomposite was developed by using L-cysteine and vitamin C as reductants under mild reaction conditions. The rGO-AuNP material showed a highly selective separation ability for 6 naturally occurring aflatoxins, which are easily adsorbed onto traditional graphene materials but are difficult to be desorbed. The specificity of the nanocomposite was evaluated in the separation of 6 aflatoxin congeners (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1 and aflatoxin M2) from 23 other biotoxins (including, ochratoxin A, citrinin, and deoxynivalenol). The results indicated that this material was specific for separating aflatoxin congeners. The synthesized material was further validated by determining the recovery (77.6–105.0%), sensitivity (limit of detection in the range of 0.05–0.21 μg kg−1), and precision (1.5–11.8%), and was then successfully applied to the separation of aflatoxins from real-world maize, wheat and rice samples.
Deconvolution of Transcriptional Networks in Post-Traumatic Stress Disorder Uncovers Master Regulators Driving Innate Immune System Function
Authors: Doostparast Torshizi Abolfazl , Wang Kai 
Published: October 2017

AbstractPost-Traumatic Stress Disorder (PTSD) is a psychiatric disorder that develops in individuals experiencing a shocking incident, but the underlying disease susceptibility gene networks remain poorly understood. Breen et al. conducted a Weighted Gene Co-expression Network Analysis on PTSD, and identified a dysregulated innate immune module associated with PTSD development. To further identify the Master Regulators (MRs) driving the network function, here we deconvoluted the transcriptional networks on the same datasets using ARACNe (Algorithm for Reconstruction of Accurate Cellular Networks) followed by protein activity analysis. We successfully identified several MRs including SOX3, TNFAIP3, TRAFD1, POU3F3, STAT2, and PML that govern the expression of a large collection of genes. Transcription factor binding site enrichment analysis verified the binding of these MRs to their predicted targets. Notably, the sub-networks regulated by TNFAIP3, TRAFD1 and PML are involved in innate immune response, suggesting that these MRs may correlate with the innate immune module identified by Breen et al. These findings were replicated in an independent dataset generated on expression microarrays. In conclusion, our analysis corroborated previous findings that innate immunity may be involved in the progression of PTSD, yet also identified candidate MRs driving the disease progression in the innate immunity pathways.
The temporal behaviour of MHD waves in a partially ionized prominence-like plasma: Effect of heating and cooling
Authors: Ballester J. L. , Carbonell M. , Soler R. , Terradas J. 
Published: October 2017

Context.During heating or cooling processes in prominences, the plasma microscopic parameters are modified due to the change of temperature and ionization degree. Furthermore, if waves are excited on this non-stationary plasma, the changing physical conditions of the plasma also affect wave dynamics.Aims.Our aim is to study how temporal variation of temperature and microscopic plasma parameters modify the behaviour of magnetohydrodynamic (MHD) waves excited in a prominence-like hydrogen plasma.Methods.Assuming optically thin radiation, a constant external heating, the full expression of specific internal energy, and a suitable energy equation, we have derived the profiles for the temporal variation of the background temperature. We have computed the variation of the ionization degree using a Saha equation, and have linearized the single-fluid MHD equations to study the temporal behaviour of MHD waves.Results.For all the MHD waves considered, the period and damping time become time dependent. In the case of Alfvén waves, the cut-off wavenumbers also become time dependent and the attenuation rate is completely different in a cooling or heating process. In the case of slow waves, while it is difficult to distinguish the slow wave properties in a cooling partially ionized plasma from those in an almost fully ionized plasma, the period and damping time of these waves in both plasmas are completely different when the plasma is heated. The temporal behaviour of the Alfvén and fast wave is very similar in the cooling case, but in the heating case, an important difference appears that is related with the time damping.Conclusions.Our results point out important differences in the behaviour of MHD waves when the plasma is heated or cooled, and show that a correct interpretation of the observed prominence oscillations is very important in order to put accurate constraints on the physical situation of the prominence plasma under study, that is, to perform prominence seismology.
Vibrationally excited water emission at 658 GHz from evolved stars
Authors: Baudry A. , Humphreys E. M. L. , Herpin F. , Torstensson K. , Vlemmings W. H. T. , Richards A. M. S. , Gray M. D. , De Breuck C. , Olberg M. 
Published: October 2017

Context. Several rotational transitions of ortho- and para-water have been identified toward evolved stars in the ground vibrational state as well as in the first excited state of the bending mode (v2 = 1 in (0, 1, 0) state). In the latter vibrational state of water, the 658 GHz J = 11,0−10,1 rotational transition is often strong and seems to be widespread in late-type stars.
Aims. Our main goals are to better characterize the nature of the 658 GHz emission, compare the velocity extent of the 658 GHz emission with SiO maser emission to help locate the water layers and, more generally, investigate the physical conditions prevailing in the excited water layers of evolved stars. Another goal is to identify new 658 GHz emission sources and contribute in showing that this emission is widespread in evolved stars.
Methods. We have used the J = 11,0−10,1 rotational transition of water in the (0, 1, 0) vibrational state nearly 2400 K above the ground-state to trace some of the physical conditions of evolved stars. Eleven evolved stars were extracted from our mini-catalog of existing and potential 658 GHz sources for observations with the Atacama Pathfinder EXperiment (APEX) telescope equipped with the SEPIA Band 9 receiver. The 13CO J = 6−5 line at 661 GHz was placed in the same receiver sideband for simultaneous observation with the 658 GHz line of water. We have compared the ratio of these two lines to the same ratio derived from HIFI earlier observations to check for potential time variability in the 658 GHz line. We have compared the 658 GHz line properties with our H2O radiative transfer models in stars and we have compared the velocity ranges of the 658 GHz and SiO J = 2−1, v = 1 maser lines.
Results. Eleven stars have been extracted from our catalog of known or potential 658 GHz evolved stars. All of them show 658 GHz emission with a peak flux density in the range ≈50–70 Jy (RU Hya and RT Eri) to ≈2000–3000 Jy (VY CMa and W Hya). Five Asymptotic Giant Branch (AGB) stars and one supergiant (AH Sco) are new detections. Three AGBs and one supergiant (VY CMa) exhibit relatively weak 13CO J = 6−5 line emission while o Ceti shows stronger 13CO emission. We have shown that the 658 GHz line is masing and we found that the 658 GHz velocity extent tends to be correlated with that of the SiO maser suggesting that both emission lines are excited in circumstellar layers close to the central star. Broad and stable line profiles are observed at 658 GHz. This could indicate maser saturation although we have tentatively provided first information on time variability at 658 GHz.
Space-time evolution of a large field of pockmarks in the Bay of Concarneau (NW Brittany)
Authors: Baltzer Agnès , Reynaud Marine , Ehrhold Axel , Fournier Jérôme , Cordier Céline , Clouet Hélène 
Published: October 2017

About a decade ago, a large field of pockmarks, covering an overall area of 36 km2was discovered in water depths of < 30 m in the central part of the Bay of Concarneau (Southern Brittany, France). This field, composed of features from 5 m to 35 m in diameter and < 1 m in depth, is characterized by unusual high densities of pockmarks, up to 5840 per square kilometre. Geophysical data correlated with sedimentary samples acquired in 2005 and 2009 show that pockmarks and their immediate surroundings are associated with dense tubes benches cover, built by a filter-feeding amphipod:Haploops nirae, forming original benthic communities. Two complementary surveys were carried out in April 2011 (Pock and Ploops) and April 2014 (Pock and Tide), on the Oceanographic Vedette (O/V) Haliotis (Ifremer/Genavir), to map the limit of the pockmarks andHaploopsfields with the maximum accuracy. The link between the presence of theHaploops niraecommunities and the occurrence of pockmarks /gas was then established and the proposed hypothesis was that tidal cycles may provide a good candidate for a short-term (monthly) triggering mechanism of fluid expulsion (Baltzer A, Ehrhold A, Rigolet C, Souron A, Cordier C, Clouet H. 2014. Geophysical exploration of an active pockmark field in the Bay of Concarneau, southern Brittany, and implications for resident suspension feeders.Geo-Marine Letters,34, 215–230). Due to the high-level precision (50 cm) of the positioning system (Magellan Aquarius Ixsea Hydrins) coupled with the RTK attitude system, these new bathymetric and imagery maps together with the sub-bottom Chirp profiles, allow us to compare the data sets from April 2011 and April 2014. The superimposition of the two data sets shows that the distribution of the pockmarks remains similar between these 2 dates (i.e.for 3 years), for the group of large, widely scattered pockmarks, which are deeply rooted in the Holocene palaeo-valley infills and for the group of pockmarks identified as the trawl-scour pockmarks, initiated by trawling action. Most of the pockmarks present very recent shapes without any infilling but sonar imagery reveals that some of them have been covered by a thin muddy layer, thereby reflecting, at least, a temporary cessation of expulsion or a different activity. Chirp profiles indicate some acoustic flares above the pockmarks, revealing gas/fluid expulsion. Different gas clues within the sedimentary column, such as acoustic turbidity, enhanced gas reflectors (EGR), chimneys pipes, occur at exactly the same places on the chirp seismic profiles from 2011 and 2014. Therefore, contrary to most examples described in the literature, this pockmarks field is still active.
Revision Total Knee Arthroplasty with Metaphyseal Sleeves without Stem: Short-Term Results
Authors: Stefani Giacomo , Mattiuzzo Valerio , Prestini Greta 
Published: October 2017

Purpose The aim of this study was to evaluate the efficacy of revision total knee arthroplasty (TKA) with cementless metaphyseal sleeves without stems either in the femoral or tibial side or in both.
Methods In this retrospective study, 51 patients (51 knees) operated in the period 2010 to 2015 met the above-mentioned criteria and were invited to a medical examination including X-rays. Forty-six were available for the study. Mean follow-up was 37 months. Knee Society score (KSS) (objective knee score), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, and range of motion (ROM) were used as outcome scores and compared with baseline values. X-rays were also examined and compared with postoperative exams to evaluate the presence of loosening, radiolucent lines, and bone ingrowth. Satisfaction of the patients was also investigated using a linear scale from 1 to 10.
Results KSS improved from 39 to 77 (p < 0.01); WOMAC score improved from 76 to 41 (p < 0.01). Twenty-four (52%) patients were satisfied, 15 (32%) were partially satisfied, and 7 (16%) were unsatisfied. ROM improved from 93 to 96 degrees (nonsignificant difference). X-rays showed no loosening of the implants, radiolucent lines in 4 patients (3 of them were asymptomatic) and bone ingrowth in 43 out of 46 patients.
Conclusion In this short-term retrospective study, the use of sleeves without stem was a safe and effective procedure in revision TKA. We found a significant improvement in clinical results compared with baseline values and no signs of implant loosening.
Level of Evidence Level IV, therapeutic case series.
Cigarette Smoke Extract Inhibits Platelet Aggregation by Suppressing Cyclooxygenase Activity
Authors: Kashiwagi Hitoshi , Yuhki Koh-ichi , Imamichi Yoshitaka , Kojima Fumiaki , Kumei Shima , Higashi Tsunehito , Horinouchi Takahiro , Miwa Soichi , Narumiya Shuh , Ushikubi Fumitaka 
Published: October 2017

AbstractThe results of studies that were performed to determine whether cigarette smoking affects platelet function have been controversial, and the effects of nicotine- and tar-free cigarette smoke extract (CSE) on platelet function remain to be determined. The aim of this study was to determine the effect of CSE on platelet aggregation and to clarify the mechanism by which CSE affects platelet function. CSE inhibited murine platelet aggregation induced by 9,11-dideoxy-9α,11α-methanoepoxy-prosta-5Z,13E-dien-1-oic acid (U-46619), a thromboxane (TX) A2 receptor agonist, and that induced by collagen with respective IC50 values of 1.05 ± 0.14% and 1.34 ± 0.19%. A similar inhibitory action of CSE was also observed in human platelets. CSE inhibited arachidonic acid–induced TXA2 production in murine platelets with an IC50 value of 7.32 ± 2.00%. Accordingly, the inhibitory effect of CSE on collagen-induced aggregation was significantly blunted in platelets lacking the TXA2 receptor compared with the inhibitory effect in control platelets. In contrast, the antiplatelet effects of CSE in platelets lacking each inhibitory prostanoid receptor, prostaglandin (PG) I2 receptor and PGE2 receptor subtypes EP2 and EP4, were not significantly different from the effects in respective control platelets. Among the enzymes responsible for TXA2 production in platelets, the activity of cyclooxygenase (COX)-1 was inhibited by CSE with an IC50 value of 1.07 ± 0.15% in an uncompetitive manner. In contrast, the activity of TX synthase was enhanced by CSE. The results indicate that CSE inhibits COX-1 activity and thereby decreases TXA2 production in platelets, leading to inhibition of platelet aggregation.