bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an anaerobic ammonium oxidation (ANAMMOX) process reactor for aquaculture wastewater treatment
Authors: Nabilah Ismail Ismafatin , Zulkarnaini Zulkarnaini , Umar Ziyaulhaq , Ismail Shahrul Bin 
Published: September 2024

ABSTRACT

Treatment of wastewater from aquaculture with high nitrogen compound concentrations would contribute to the sustainable aquaculture industry. The anaerobic ammonium oxidation (ANAMMOX) mechanism was a potential technology considering its capability to directly convert ammonium and produce nitrogen gas under anaerobic conditions. This research aimed to cultivate locally isolated ANAMMOX bacteria from marine samples while exploring the effectiveness of nitrogen removal biologically in a developed fixed film bioreactor. The laboratory-scale experiment used a sludge sample from a local anaerobic lagoon as inoculum. The nitrogen removal efficiency and the development of ANAMMOX bacteria indicate the ANAMMOX process's effectiveness. After 200 days of reactor operation, the ammonium removal efficiency (ACE) was 70.39 and 82.49% of nitrogen removal efficiency (NRE). The nitrogen loading rate (NLR) value was 0.30 kg-N/m3.d and the highest nitrogen removal rate (NRR) was 0.125 kg-N/m3.d. The ratio of stoichiometric for this study was 1 mol ammonium to 1.00 mol nitrite removal to the 0.21 nitrate production (1:1.00:0.21). The findings from this study could establish the implementation of the ANAMMOX technology on nitrogen removal biologically for the remediation of aquaculture wastewater toward an industrial scale.
Competence Regulation in Streptococcus pneumoniae and the Competence-targeted Anti-pneumococcal Strategies
Authors: Zhang Yapeng , Guo Shaowei , li Yimin , Zhou Linying , Wu Yong 
Published: September 2024

Abstract:
Natural transformation refers to the process in which bacteria acquire new
traits by uptaking naked DNA from the environment and integrating it into their genome
through homologous recombination when they are in the specialized physiological state
of competence. The natural transformation was first described in Streptococcus pneumoniae.
Since Frederick Griffith first described natural transformations in S. pneumoniae in
1928, this phenomenon has been studied extensively. Induction of competence before natural
transformation has been reported to involve about 10% of the pneumococcal
genome. In addition to natural transformation, multiple physiological processes are involved,
including biofilm formation, bacteriocin production, and fratricide. In this review,
we summarized current knowledge about natural transformation in S. pneumoniae
and described its competence regulation mechanism. This review also introduces the development
of novel drugs and vaccines against S. pneumoniae infection by utilizing the
existing knowledge of competence and natural transformation.
Development of Mixed Micelles for Enhancing Fenretinide Apparent Solubility and Anticancer Activity Against Neuroblastoma Cells
Authors: Zuccari Guendalina , Zorzoli Alessia , Marimpietri Danilo , Alfei Silvana 
Published: September 2024

Introduction/Objectives:
The purpose of the study was to evaluate the suitability of
mixed micelles prepared with D-α-tocopheryl polyethylene glycol succinate (TPGS) and 1,2-
distearoyl-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-PEG) to
encapsulate the poorly soluble anticancer drug fenretinide (4-HPR).

Methods:
After assaying the solubilization ability of the surfactants by the equilibrium method, the
micelles were prepared using the solvent casting technique starting from different 4-HPR:TPGS:
DSPE-PEG w/w ratios. The resulting formulations were investigated for their stability under storage
conditions and upon dilution, modelling the reaching of physiological concentrations after intravenous
administration. The characterization of micelles included the determination of DL%, EE %,
particle size distribution, Z-potential, and thermal analysis by DSC. The cytotoxicity studies were
performed on HTLA-230 and SK-N-BE-2C neuroblastoma cells by the MTT essay.

Results:
The colloidal dispersions showed a mean diameter of 12 nm, negative Zeta potential, and a
narrow dimensional distribution. 4-HPR was formulated in the mixed micelles with an encapsulation
efficiency of 88% and with an increment of the apparent solubility of 363-fold. The 4-HPR entrapment
remained stable up to the surfactants’ concentration of 2.97E-05 M. The loaded micelles exhibited
a slow-release behaviour, with about 28% of the drug released after 24 h. On the most resistant
SK-N-BE-2C cells, the encapsulated 4-HPR was significantly more active than free 4-HPR in reducing
cell viability.

Conclusion:
Loaded micelles demonstrated their suitability as a new adjuvant tool potentially useful
for the treatment of neuroblastoma.
Plumbagin's Healing Effect on Motor Impairment in Rotenone-toxified Rodents
Authors: Verma Aanchal , Goyal Ahsas 
Published: September 2024

Background:
Parkinson's disease is an illness marked by a gradual mitigation of dopamine
neurons within the substantia nigra, which eventually leads to a deficiency of dopamine
that further gives rise to mobility as well as cognitive impairments. Through long-established
traditions, a wide array of Traditional Chinese Medicines (TCM) have undergone testing and are
employed to avoid neurodegenerative disorders. Plumbagin is the primary active component of a
medication called Baihua Dan or Plumbago zeylanica L., which is clinically used in China.

Objectives:
This study investigated plumbagin-induced alterations in a Parkinson's disease rat
model instigated by subcutaneous rotenone injection.

Methods:
Male rats were administered subcutaneous injections of rotenone at a dosage of 1.5
mg/kg, followed by the treatment with varying doses of plumbagin (10, 20, and 40 mg/kg)
through the oral route. The rats underwent various motor ability tests, including the actophotometer,
rotarod, open field, beam walk, gait evaluation, ability to grip, and catalepsy bar tests. Furthermore,
the brain dopamine level was then estimated for the extracted tissues. Also, through
molecular docking, the binding effectiveness of plumbagin was assessed for human MAO-B.
After that, plumbagin was put through 100 ns of molecular dynamic simulations to examine the
stability of its conformational binding to the target protein. Furthermore, ADMET tests were
used to verify Plumbagin's druggability.

Results:
Plumbagin was found to alleviate rotenone-induced motor abnormalities and restore
brain dopamine levels. Furthermore, plumbagin showed excellent interactions with MAO-B
(monoamine oxidase-B) when compared with selegiline (a standard drug for Parkinson’s disease).

Conclusion:
These findings underscore the potential therapeutic efficacy of plumbagin in mitigating
behavioural deficits in rotenone-induced rodents. Considering this, plumbagin might be a
feasible pharmacological strategy for the control of rotenone-triggered behavioural impairment
in rats (in vivo), and it might display interesting interactions with MAO-B (in silico).
FORTIFICATION OF FREE-RANGE CHICKEN PASTA TO INCREASE CALCIUM CONTENT IN THE MAKING OF PULI CRACKERS
Authors: Sunarni Sunarni 
Published: September 2024

This research aims to: (1) find a formula for free-range chicken puli crackers that are rich in nutrients; (2) finding the formula for free-range chicken puli crackers that is most liked by the panelists; (3) determine the nutritional content of free-range chicken puli crackers using the proximate method; (4) determine changes in calcium levels in free-range chicken puli crackers products during processing; (5) knowing the portion of free-range chicken puli crackers that is able to meet calcium requirements; and (6) determine the expiry date for free-range chicken puli crackers.
This research was carried out in three stages, including making free-range chicken puli crackers, liking tests, and analyzing nutritional content. The making of free-range chicken puli crackers and the likelihood test were carried out at the PTBB FT UNY laboratory between March until June 2010, while the nutritional content analysis was carried out at the CV Chem-Mix Pratama laboratory in July 2010. The liking test was carried out using the Hedonic method on 30 panelists, consisting of 25 students and five lecturers from the Culinary Engineering Education Study Program PTBB FT UNY. The analysis of nutritional content includes proximate analysis and fiber content. Proximate analysis includes water content, ash content, fat content, protein content and carbohydrate content. Apart from that, an analysis of the fiber content was also carried out. The calculation data were analyzed descriptively statistically using Anava (Analysis of Variance).
The results of this research are: (1) variations in the product formula for free-range chicken puli crackers by 10%, 30% and 50% (chicken); (2) the preference test of the panelists showed that the most preferred native chicken puli crackers were the 30% formula; (3) the nutritional content of free-range chicken puli crackers through proximate analysis obtained data namely water content 2.4929%, ash content 3.0289%, protein content 15.1557%, fat content 9.7012%, and carbohydrate content 66.7105% ; (4) the calcium content from fresh free-range chicken to pre-cooked free-range chicken decreased by 0.8532%, and the calcium content from pre-cooked local chicken to free-range chicken crackers decreased by 0.5170%. The calcium content of free-range chicken puli crackers is 1.2062%; (5) portion of free-range chicken puli crackers, namely each serving weighs 2.5 g (consisting of 1 piece), and the weight of one package is 50 g containing 0.02% of
Online Ambassador Visits for Hospitalized Children With Cancer: Qualitative Evaluation of Implementation
Authors: Boensvang Natasha Nybro , Weibel Mette , Wakefield Claire E , Bidstrup Pernille Envold , Olsen Marianne , Nissen Karin Bækgaard , Spager Vibeke , Fridh Martin Kaj , Larsen Hanne Bækgaard 
Published: September 2024

Background
Children with cancer or cancer-like disease risk treatment-related isolation, which can negatively impact their peer relationships and social competencies and exacerbate their loneliness. During the COVID-19 pandemic, increased online socialization became the new normal imposed by national isolation guidelines. To adhere to the treatment-related isolation guidelines, children with cancer were offered online classmate “ambassador” visits during hospitalization.

Objective
This study aimed to identify facilitators and barriers to online classmate “ambassador” visits during children with cancer’s hospitalization through a qualitative descriptive process evaluation using the Consolidated Framework for Implementation Research.

Methods
From January to April 2022, we conducted 39 individual semistructured interviews with hospitalized children (n=16), their classmates (n=16), teachers from their schools (n=3), and study nurses (n=4) from involved hospitals. Most interviews (n=37, 95%) were conducted online using Microsoft Teams or Google Meet, while 2 (5%) interviews were conducted in person at the participants’ residences. This approach allowed us to gain a broad understanding of the facilitators and barriers to online ambassador visits.

Results
We identified four themes: (1) working together, (2) ensuring participation, (3) staying connected, and (4) together online. The themes are described in terms of facilitators and barriers to online ambassador visits with 3 Consolidated Framework for Implementation Research domains: innovation, individuals, and the implementation process.

Conclusions
Addressing the social needs of hospitalized children through online visits with their classmates may be relevant when one-on-one meetings are problematic. The online visits are highly dependent on collaboration between study nurses and teachers and assessing the needs of the hospitalized children. While a high degree of adult engagement and a stable internet connection are pivotal, these online visits can promote much-needed social interaction between children across physical settings.
Quantifying the Enhancement of Sarcopenic Skeletal Muscle Preservation Through a Hybrid Exercise Program: Randomized Controlled Trial
Authors: Guo Hongzhi , Cao Jianwei , He Shichun , Wei Meiqi , Meng Deyu , Yu Ichen , Wang Ziyi , Chang Xinyi , Yang Guang , Wang Ziheng 
Published: September 2024

Abstract

Background
Sarcopenia is characterized by the loss of skeletal muscle mass and muscle function with increasing age. The skeletal muscle mass of older people who endure sarcopenia may be improved via the practice of strength training and tai chi. However, it remains unclear if the hybridization of strength exercise training and traditional Chinese exercise will have a better effect.

Objective
We designed a strength training and tai chi exercise hybrid program to improve sarcopenia in older people. Moreover, explainable artificial intelligence was used to predict postintervention sarcopenic status and quantify the feature contribution.

Methods
To assess the influence of sarcopenia in the older people group, 93 participated as experimental participants in a 24-week randomized controlled trial and were randomized into 3 intervention groups, namely the tai chi exercise and strength training hybrid group (TCSG; n=33), the strength training group (STG; n=30), and the control group (n=30). Abdominal computed tomography was used to evaluate the skeletal muscle mass at the third lumbar (L3) vertebra. Analysis of demographic characteristics of participants at baseline used 1-way ANOVA and χ2 tests, and repeated-measures ANOVA was used to analyze experimental data. In addition, 10 machine-learning classification models were used to calculate if these participants could reverse the degree of sarcopenia after the intervention.

Results
A significant interaction effect was found in skeletal muscle density at the L3 vertebra, skeletal muscle area at the L3 vertebra (L3 SMA), grip strength, muscle fat infiltration, and relative skeletal muscle mass index (all P values were <.05). Grip strength, relative skeletal muscle mass index, and L3 SMA were significantly improved after the intervention for participants in the TCSG and STG (all P values were <.05). After post hoc tests, we found that participants in the TCSG experienced a better effect on L3 SMA than those in the STG and participants in the control group. The LightGBM classification model had the greatest performance in accuracy (88.4%), recall score (74%), and F1-score (76.1%).

Conclusions
The skeletal muscle area of older adults with sarcopenia may be improved by a hybrid exercise program composed of strength training and tai chi. In addition, we identified that the LightGBM classification model had the best performance to predict the reversion of sarcopenia.
Hubungan Tingkat Pengetahuan Lansia terhadap Penanganan Pertama Kejadian Ikutan Pasca Imunisasi (KIPI) Vaksin Covid-19
Authors: Wahyuddin Munifah , Alifia Putri Febriyanti  , Nurshalati Tahar  , Khaerani  , Andi Arwini Puspitasari  
Published: September 2024

Abstrak
Pendahuluan: Di Indonesia sedang mengalam krisis pandemi COVID-19 dan kelompok geriatri merupakan kelompok yang rentan atau mudah terjangkit virus COVID-19 ini. Solusi yang diberikan pemerintah yaitu adanya perlakuan vaksinasi sebagai pencegahan penyebaran virus. Namun setelah pemberian vaksin terdapat gejala-gejala yang tidak dapat dihindari, gejala tersebut dinamakan KIPI (Kejadian Ikutan Pasca Imunisasi). Tujuan: Untuk mengetahui hubungan tingkat pengetahuan geriatri terhadap penanganan pertama KIPI vaksin covid-19. Metode: Penelitian ini dilakukan dengan metode observasional korelatif dengan pendekatan cross-sectional. Sampel lansia yang memenuhi kriteria sebanyak 162 responden. Hasil: Berdasarkan penelitian yang telah dilakukan, diperoleh hasil sekitar 54 responden (33.33%) dengan kategori baik pada pengetahuan dan penanganan KIPI, 97 responden (59,9%) dengan kategori cukup pada penanganan pertama KIPI dan 11 responden (6.8%) termasuk kategori kurang pada pengetahuan dan penanganan KIPI. Hasil analisis bivariat dengan uji Chi Square didapatkan p value =0,000 < 0,05 yang artinya adanya hubungan yang bermakna terhadap Tingkat pengetahuan dengan penanganan pertama Kejadian Ikutan Pasca Imunisasi (KIPI) vaksin Covid-19. Kesimpulan: Tingkat pengetahuan memiliki peranan yang penting dalam penanganan pertama kejadian ikutan pasca imunasasi (KIPI), sehingga informasi dan edukasi sangat dibutuhkan oleh masyarakat mengenai manfaat, efek samping dan penanganan yang tepat terkait vaksin COVID-19 terutama pada lansia.

Kata Kunci: Covid-19, Geriatri, KIPI, Tingkat Pengetahuan, Vaksin.
Determinants of supraventricular extra beats in elite athletes practicing different sporting disciplines
Authors: Di Gioia Giuseppe , Vespasiano Francesca , Maestrini Viviana , Monosilio Sara , Segreti Andrea , Lemme Erika , Squeo Maria Rosaria , Serdoz Andrea , Pelliccia Antonio 
Published: September 2024

Background
Supraventricular extra beats (SVEB) are frequently observed in athletes but data on significance, prognostic role and correlation with cardiac remodeling are contrasting. It is uncertain whether SVEB may indicate the development of more complex arrhythmias and the need for closer monitoring is undetermined. The aim was to assess the prevalence and clinical significance of BESV in Olympic athletes of different sporting disciplines, evaluating potential correlations with cardiac remodeling and clinical features.

Methods
We enrolled athletes who participated at 2012–2022 Olympic Games, submitted to physical examination, blood tests, echocardiography and exercise tests, categorized into power, skills, endurance and mixed disciplines.

Results
We studied 1492 elite athletes: 56% male individuals, mean age 25.8 ± 5.1 years; 29.5% practiced power, 12.3% skills, 21% endurance and 37.2% mixed disciplines. At exercise-stress tests, 6.2% had SVEB, mostly single beats. SVEB were not influenced by anthropometrics or blood test results. They were more common in male individuals (77.4 vs. 54.6%, P < 0.0001) and older athletes (27.1 ± 5.7 vs. 25.7 ± 5.1, P = 0.01). In male athletes with SVEB, higher left atrial volumes were observed (24.2 ± 7.3 vs. 22.2 ± 7.1 ml/m2, P = 0.03). No differences were found in terms of sporting discipline: despite larger left atrial dimensions in aerobic disciplines, SVEB rates were similar in different sporting disciplines (6.1% endurance, 6.3% mixed, 5.2% power and 8.7% skills; P = 0.435).

Conclusion
SVEB were more common in older, male athletes and associated with higher left atrial volume (especially in male individuals) regardless of sport practiced. Athletes with greater left atrial volume and SVEB are supposed to have higher risk, in middle age, of developing more complex arrhythmias.
Coping Strategies For the Prevention of Social Violence in University Students
Authors: Palacios-Serna Lina Iris , Obando-Peralta Ena Cecilia , Durand-Ascárate Luis Augusto , Velazco-Reyes Benjamín , Soto Idana Beroska Rincón 
Published: September 2024

Purpose: The objective is to point out the scientific evidence available on coping strategies, based on a systematic review of relevant articles existing in the scientific literature.

Theoretical framework: The university environment is not alien to the processes of violence, harassment and discrimination in society as a whole. In this context, the analysis of the relationships that unfold in university communities reveals practices that materialize symbolic violence originating in inequalities, in historical and structural social hierarchies. (Franco & Puglia, 2023). Hence, the coping responses are related to the way the subject perceives events, as well as the internal resources available (Diaz, Y., 2010 and Coppari et al., 2019).

Method: Its design is a systematic review without meta-analysis. From a total of 139 initial references detected in the Web of Science, Scopus, Scielo, Redalyc, Dialnet, and Google Scholar databases, 22 studies were selected that met the defined quality and selection criteria. The PRISMA matrix was used for coding. The coping strategies category and subcategories such as author, year of publication, country; study population; design; the various types of instruments that measure coping strategies and the main results were used.

Results and discussion: As a result of the review of the articles, 70% of university students show a predominance of coping strategies related to problem solving, 25% refer to strategies for evaluating stressful situations and developing a plan and executing tasks. The other 5% range in the use of strategies for seeking professional help and religious practice. On the other hand, 60% of the studies report that students show problem-focused strategies and 40% stand out in the use of emotional-focused strategies.

Conclusion: According to the results, it is inferred that the use of coping strategies helps prevent violence because it allows highlighting the assertive way of how to act by mediating the cognitive and emotional aspects in response to adverse, difficult, pressure situations.

Implications: Coping strategies allow to cope with difficult situations and help create healthy environments in a population that requires the implementation of acceptable social behaviors that promote peace and the sustainability of a peaceful society with less violence.

Value: Therefore, the review carried out is relevant, because it emphasizes how coping strategies allow to cope with difficult situations and help create socially healthy environments, even more so in populations that require the implementation of behaviors that promote peace, justice and inclusion.