bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nell‐1‐ΔE, a novel transcript of Nell‐1, inhibits cell migration by interacting with enolase‐1
Authors: Zhao Huaxiang , Qin Xueyan , Zhang Qian , Zhang Xinli , Lin Jiuxiang , Ting Kang , Chen Feng 
Published: February 2018

AbstractNELL‐1 is a secreted protein that was originally found to be upregulated in pathologically fusing and fused sutures in non‐syndromic unilateral coronal synostosis patients. Apart from the ability of NELL‐1 to promote osteogenesis in long and craniofacial bones, NELL‐1 reportedly inhibits the formation of several benign and malignant tumors. We previously identified a novel transcript of Nell‐1 that lacked a calcium‐binding epidermal growth factor (EGF)‐like domain compared with full‐length Nell‐1; this new transcript was named Nell‐1‐ΔE. Three obvious structural differences between these two isoforms were revealed by homology modeling. Furthermore, the recombinant Nell‐1‐ΔE protein, but not the full‐length Nell‐1 protein, inhibited cell migration in vitro. However, full‐length Nell‐1 and Nell‐1‐ΔE proteins were present in similar subcellular locations and displayed similar expression patterns in both the intracellular and extracellular spaces. The results from the co‐immunoprecipitation and liquid chromatography/tandem mass spectrometry analyses using two cell lines demonstrated that Nell‐1‐ΔE but not full‐length Nell‐1 interacted with enolase‐1 in the extracellular spaces of both cell lines. The results of wound healing assays using ENO‐1‐overexpressing cells treated with full‐length Nell‐1/Nell‐1‐ΔE suggested that Nell‐1‐ΔE inhibited cell migration by interacting with ENO‐1. Our study indicated that the novel transcript Nell‐1‐ΔE, but not full‐length Nell‐1, might be a candidate tumor suppressor factor for basic research and clinical practice.
Neonatal treatment with fluoxetine improves mitochondrial respiration and reduces oxidative stress in liver of adult rats
Authors: Simões‐Alves Aiany C. , Silva‐Filho Reginaldo C. , Braz Glauber R.F. , Silva Severina C.A. , da Silva Aline I. , Lagranha Claudia J. , Fernandes Mariana P. 
Published: February 2018

AbstractRecent studies have shown that exposure to fluoxetine treatment induces excessive production of ROS, and alters the antioxidant defense system in various tissues and cell types, mainly the liver. When fluoxetine is administered intraperitoneally, the drug rapidly reaches high concentrations in the liver, has potentially multiple toxic effects on energy metabolism in rat liver mitochondria. The aim of this study was to evaluate the effect of pharmacological treatment with fluoxetine during critical period for development on the mitochondrial bioenergetics and oxidative stress in liver of rat adult. To perform this study, the rat pups received Fx, or vehicle (Ct) from postnatal day 1 to postnatal day 21 (ie, during lactation period). We evaluated mitochondrial oxygen consumption, respiratory control ratio, ROS production, mitochondrial swelling by pore opening, oxidative stress biomarkers, and antioxidant defense in liver of rats at 60 days of age. Our studies have shown, that treatment with Fx during the lactation period resulted in reduced body mass gain, improvement of the mitochondrial respiratory capacity, induced higher mitochondrial resistance to calcium ion preventing the mitochondrial permeability transition pore opening, as well as decreased oxidative stress biomarkers, and increased the SH levels and enzymes antioxidant activities (SOD, CAT, GST) in liver of treated rats at 60 days of age. These findings suggest that pharmacological treatment with fluoxetine during critical period of development result in positive changes in liver of rats, as improvement of the mitochondrial bioenergetics and hepatic oxidative metabolism that persist in adulthood.
Temporal dominance of sensations and preferences of Brazilians and Slovakians: A cross‐cultural study of cachaças stored with woods from the Amazon rainforest
Authors: Simioni Síntia Carla Corrêa , Tovar Diego Mercado , Rodrigues Jéssica Ferreira , de Souza Vanessa Rios , Nunes Cleiton Antônio , Vietoris Vladimir , Pinheiro Ana Carla Marques 
Published: February 2018

AbstractBACKGROUNDBrazilians and Slovakians evaluated the temporal profile and the acceptability of cachaça stored with different woods (Cumarurana (CM), Jatobá (JT) and, Louro‐vermelho (LV), which are found in the Amazon rainforest, and also oak), with the aim of performing a cross‐cultural comparison of the dynamic profile of the attributes perceived in the cachaças and the sensorial acceptance of the samples.RESULTSImportant differences were observed between the temporal sensorial profiles generated by the two groups and their preferences. Brazilians preferred cachaças stored with the traditional wood, oak, followed by those stored with JT and CM. In contrast, Slovakians preferred cachaças stored with JT, followed by those stored with LV and oak. For both countries, the dominance of wood flavor and vanilla attributes at the end of the analysis time was positively associated with acceptance, while the dominance of off‐flavors and the wood flavor attribute at the beginning of the analysis time was negatively associated with acceptance for Brazilians and Slovakians, respectively.CONCLUSIONBrazilians preferred cachaça stored with oak wood, and Slovakians preferred cachaça stored with JT wood, with acceptability being strongly associated with the dominance of wood flavor and vanilla attributes at the end of the evaluation time. © 2018 Society of Chemical Industry
Effect of nanoparticles of silver on redox status and the accumulation of Ag in chicken tissues
Authors: Kulak Ewelina , Ognik Katarzyna , Stępniowska Anna , Drażbo Aleksandra 
Published: February 2018

AbstractBACKGROUNDThe aim of the study was to verify the hypothesis that there are doses of silver nanoparticles (Ag‐NPs) that improve antioxidant defence in chickens without compromising their growth performance and health.RESULTSThe results of our study suggest that, irrespective of the concentration, the administration of Ag‐NPs (5 nm) at a dose not exceeding 54 mg/bird has no negative effect on the growth performance of chickens. The administration of Ag‐NPs (5 nm) in the amount of 2.87 to 63.74 mg/bird does not cause silver to accumulate in the breast muscle. The ingestion of 2.87 mg/bird was found to result in the accumulation of this element in the wall of the small intestine and in the liver, and a further increase in the dose increases the accumulation of Ag in these tissues. Accumulation of Ag in the heart of the chickens was not observed until the dose reached 22.5 mg/bird.CONCLUSIONOur research has shown that the application of Ag‐NPs (5 nm), especially at doses greater than the ‘no observable adverse effect level’ (NOAEL) of 9.47 mg/bird, can induce oxidative reactions in the blood, small intestinal wall, liver and breast muscle of chickens. © 2018 Society of Chemical Industry
Clear Cell Hidradenoma: Characteristic Imaging Features on Ultrasonography, Computed Tomography, and Magnetic Resonance Imaging
Authors: Lee Joo Yeon , Kang Byeong Seong , Shim Hyun Seok , Song In Hye , Kim Misung , Lee Sang Hoon , Chung Hye Won , Lee Min Hee , Shin Myung Jin 
Published: February 2018

ObjectivesThe purpose of this study was to evaluate the imaging features of clear cell hidradenoma on ultrasonography (US), computed tomography (CT), and magnetic resonance imaging (MRI).MethodsThe radiologic and pathologic databases at 2 medical institutions were searched retrospectively from 2004 to 2016 to identify patients with a diagnosis of clear cell hidradenoma. Ultrasonographic, CT, and MRI features were described, and pathologic specimens were reviewed.ResultsThere were 5 female and 4 male patients. The mean patient age was 48.9 years (range, 28–70 years). Five patients underwent only US; 2 patients underwent only CT; 1 patient underwent both US and CT; and 1 patient underwent US and MRI. Most of the tumors were located primarily in the subcutaneous fat layer. The mean tumor size was 18.4 mm. On US, 6 masses had a heterogeneous echo texture, including an anechoic portion with protruding echogenic portions. Two masses had multiple septa in the anechoic portion. On color Doppler US, blood flow was both central and peripheral in 5 patients. All 3 cases seen on CT presented as a low‐attenuation mass with an enhanced solid internal nodule. On MRI, the mass showed heterogeneous signal intensity on T2‐weighted images and enhancement of the peripheral wall and internal solid component on contrast‐enhanced T1‐weighted images.ConclusionsClear cell hidradenoma is usually located in the subcutaneous fat layer, has a well‐defined margin, appears as a cystic mass with an internal solid nodule, and occasionally has multiple septa on US, CT, and MRI.
Managing emerging threats to spotted owls
Authors: Wan Ho Yi , Ganey Joseph L. , Vojta Christina D. , Cushman Samuel A. 
Published: February 2018

ABSTRACTThe 3 spotted owl (Strix occidentalis) subspecies in North America (i.e., northern spotted owl [S. o. caurina], California spotted owl [S. o. occidentalis], Mexican spotted owl [S. o. lucida]) have all experienced population declines over the past century due to habitat loss and fragmentation from logging. Now, the emerging influences of climate change, high‐severity fire, and barred owl (Strix varia) invasion also appear to be synergistically and differentially affecting population trends of each subspecies. Our objective was to review the existing literature on the spotted owl to describe historical and emerging threats and whether those threats have been adequately examined for each subspecies. Using 527 publications from a Web of Science search of the literature from 1900–2015, we statistically evaluated the emphasis placed on each subspecies regarding 4 influences: mechanical tree removal, fire, climate change, and barred owl invasion. There were 98 papers that explicitly examined the effects of ≥1 of these influences. Most of these papers were focused on the northern spotted owl, and for all 3 subspecies, most papers examined short‐term effects only. We used our results to identify significant information gaps relative to historical and emerging threats. Commercial timber harvesting remains a potential threat for all 3 spotted owl subspecies, but effects from forest thinning may be increasing because of the heightened emphasis on fuels reduction and forest restoration treatments on public lands. Owl response to mechanical tree removal, especially forest thinning, remains understudied. Climate change also may threaten all 3 subspecies. Changes in climate likely affect survival and reproduction of spotted owls and their prey, and alter habitat availability by affecting disturbance regimes and vegetation composition and succession, but little empirical information is available describing specific responses to climate change. The literature on response to high‐severity fire is sparse for some subspecies, primarily short‐term in nature, and not consistent. Barred owl invasion is a major threat to the northern spotted owl and the California spotted owl but does not currently threaten the Mexican spotted owl. Rigorous research on the response of spotted owls to all factors influencing population change, particularly for the Mexican spotted owl, is needed. The most useful information for predicting owl response to these threats stems primarily from long‐term studies of owl demography. The lack of such studies within the range of the Mexican spotted owl greatly limits our understanding of its population dynamics and our ability to predict the effects of various threats on Mexican spotted owl populations. For all 3 subspecies, we encourage long‐term studies of their responses to threats, using uniquely marked owls across large spatial extents to account for spatiotemporal variability in ecological conditions within and among subspecies. © 2018 The Wildlife Society.
Combined experimental and multiphase computational fluid dynamics analysis of surface textured journal bearings in mixed regime of lubrication
Authors: Morris N.J. , Shahmohamadi H. , Rahmani R. , Rahnejat H. , Garner C.P. 
Published: February 2018

AbstractThis paper investigates the effect of surface texturing in a partial pad journal bearing through a series of controlled experiments at operating conditions, promoting mixed or boundary regimes of lubrication. Improvements to load carrying capacity are observed under certain operating conditions. A comprehensive computational finite volume multiphase fluid dynamics analysis, including vapour transport equation and modified finite‐size cavity Rayleigh‐Plesset model, is used to study the effect of indented surface textures in the microscale contact domain and within the individual textures themselves. The results show improved conditions with a textured journal through promotion of micro‐hydrodynamic effect, delaying the effect of lubricant rupture, thus extending the effective load bearing region. A very good agreement is obtained between measurements and predictions.
The high‐impact 2007 hot summer over Turkey: atmospheric‐blocking and heat‐wave episodes
Authors: Demirtaş Meral 
Published: February 2018

ABSTRACTThe 2007 summer was extraordinary in the Balkans and Turkey, with the region experiencing a very hot summer in numerous places, setting all‐time maximum temperature records and suffering destructive fires. The heat wave exhibited daily maximum temperature anomalies in excess of 14 °C in some cities. These high‐temperature anomalies can be related to a number of concurrent atmospheric and physical factors that induce persistent anticyclones, sea‐surface temperature anomalies, reduced precipitation and depleted soil moisture. Prominent atmospheric factors conducive to heat‐wave events can serve as a dynamic fingerprint and yield insight into their most important triggering and driving mechanisms. The prolonged atmospheric‐blocking high meandering over the central Mediterranean orchestrated the atmospheric circulation and led to the advection of warm air from North Africa to the Balkans and Turkey. The associated large‐scale subsidence and clear‐sky conditions resulted in temperatures surpassing 40 °C at many places. Atmospheric and physical conditions contributed positively to the very high temperatures and led to two major heat‐wave episodes in June and July. Atmospheric‐blocking analysis revealed that two blocking episodes accompanied these heat waves. Results from this analysis indicate that the atmospheric blocking was likely responsible for establishing and maintaining the hot‐weather conditions. The summer of 2007 may be considered as a strong indicator of what Turkish summers may become in future.
In silico analysis of structural modifications in and around the integrin αIIb genu caused by ITGA2B variants in human platelets with emphasis on Glanzmann thrombasthenia
Authors: Pillois Xavier , Peters Pierre , Segers Karin , Nurden Alan T. 
Published: February 2018

AbstractBackgroundStudies on the inherited bleeding disorder, Glanzmann thrombasthenia (GT), have helped define the role of the αIIbβ3 integrin in platelet aggregation. Stable bent αIIbβ3 undergoes conformation changes on activation allowing fibrinogen binding and its taking an extended form. The αIIb genu assures the fulcrum of the bent state. Our goal was to determine how structural changes induced by missense mutations in the αIIb genu define GT phenotype.MethodsSanger sequencing of ITGA2B and ITGB3 in the index case followed by in silico modeling of all known GT‐causing missense mutations extending from the lower part of the β‐propeller, and through the thigh and upper calf‐1 domains.ResultsA homozygous c.1772A>C transversion in exon 18 of ITGA2B coding for a p.Asp591Ala substitution in an interconnecting loop of the lower thigh domain of αIIb in a patient with platelets lacking αIIbβ3 led us to extend our in silico modeling to all 16 published disease‐causing missense variants potentially affecting the αIIb genu. Modifications of structuring H‐bonding were the major cause in the thigh domain although one mutation gave mRNA decay. In contrast, short‐range changes induced in calf‐1 appeared minor suggesting long‐range effects. All result in severe to total loss of αIIbβ3 in platelets. The absence of mutations within a key Ca2+‐binding loop in the genu led us to scan public databases; three potential single allele variants giving major structural changes were identiffied suggesting that this key region is not protected from genetic variation.ConclusionsIt appears that the αIIb genu is the object of stringent quality control to prevent platelets from circulating with activated and extended integrin.
Texture analysis of cardiac cine magnetic resonance imaging to detect nonviable segments in patients with chronic myocardial infarction
Authors: Larroza Andrés , López‐Lereu María P. , Monmeneu José V. , Gavara Jose , Chorro Francisco J. , Bodí Vicente , Moratal David 
Published: February 2018

PurposeTo investigate the ability of texture analysis to differentiate between infarcted nonviable, viable, and remote segments on cardiac cine magnetic resonance imaging (MRI).MethodsThis retrospective study included 50 patients suffering chronic myocardial infarction. The data were randomly split into training (30 patients) and testing (20 patients) sets. The left ventricular myocardium was segmented according to the 17‐segment model in both cine and late gadolinium enhancement (LGE) MRI. Infarcted myocardium regions were identified on LGE in short‐axis views. Nonviable segments were identified as those showing LGE ≥ 50%, and viable segments those showing 0 < LGE < 50% transmural extension. Features derived from five texture analysis methods were extracted from the segments on cine images. A support vector machine (SVM) classifier was trained with different combination of texture features to obtain a model that provided optimal classification performance.ResultsThe best classification on testing set was achieved with local binary patterns features using a 2D + t approach, in which the features are computed by including information of the time dimension available in cine sequences. The best overall area under the receiver operating characteristic curve (AUC) were: 0.849, sensitivity of 92% to detect nonviable segments, 72% to detect viable segments, and 85% to detect remote segments.ConclusionNonviable segments can be detected on cine MRI using texture analysis and this may be used as hypothesis for future research aiming to detect the infarcted myocardium by means of a gadolinium‐free approach.
Augmented Lagrangian and Galerkin least‐squares methods for membrane contact
Authors: Burman Erik , Hansbo Peter , Larson Mats G. 
Published: February 2018

SummaryIn this paper, we propose a stabilized finite element method for the numerical solution of contact between a small deformation elastic membrane and a rigid obstacle. We limit ourselves to friction‐free contact, but the formulation is readily extendable to more complex situations.
A 3‐node C0 triangular element for the modified couple stress theory based on the smoothed finite element method
Authors: Choi J. H. , Lee B. C. 
Published: February 2018

SummaryIn this paper, a 3‐node C0 triangular element for the modified couple stress theory is proposed. Unlike the classical continuum theory, the second‐order derivative of displacement is included in the weak form of the equilibrium equations. Thus, the first‐order derivative of displacement, such as the rotation, should be approximated by a continuous function. In the proposed element, the derivative of the displacement is defined at a node using the node‐based smoothed finite element method. The derivative fields, continuous between elements and linear in an element, are approximated with the shape functions in element. Both the displacement field and the derivative field of displacement are expressed in terms of the displacement degree of freedom only. The element stiffness matrix is calculated using the newly defined derivative field. The performance of the proposed element is evaluated through various numerical examples.
Pain self‐management plus nurse‐led support in young adults with irritable bowel syndrome: Study protocol for a pilot randomized control trial
Authors: Cong Xiaomei , Ramesh Divya , Perry Mallory , Xu Wanli , Bernier Katherine M. , Young Erin E. , Walsh Stephen , Starkweather Angela 
Published: February 2018

ABSTRACTIrritable bowel syndrome (IBS) is a functional gut disorder that typically manifests in early adult years. IBS patients report that pain is the most distressing symptom with the greatest impact on quality of life. Pain‐sensitivity genes and the gut microbiome may influence severity of symptoms as well as response to self‐management (SM) interventions. Based on current understanding of the science of SM, pain neurophysiology, and the gut‐brain axis, our team developed a pain SM intervention to be added to evidence‐based self‐management instruction to increase the individual's SM knowledge and skills (self‐efficacy, self‐regulation, and goal‐setting). The purpose of this randomized controlled longitudinal pilot study is to examine the feasibility, acceptability, and preliminary effectiveness of the IBS‐pain SM intervention on IBS‐pain SM behaviors and related health outcomes. A sample of 80 young adults (age 18–29 years old) will be recruited and randomly assigned to the experimental or control group. Both groups will receive 10 electronic video modules focused on IBS‐pain SM knowledge and skills. The experimental group also will receive nurse‐led one‐on‐one phone consultations to facilitate monitoring and problem‐solving. All participants will be followed over 12 weeks. Primary outcomes will be measured at baseline, 6 weeks, and 12 weeks, including IBS‐pain SM behaviors, quality of life, and well‐being. The influence of pain‐sensitivity genes and the gut microbiome on IBS‐pain SM behaviors and health outcomes also will be assessed.
Investigating properties of poly (ether‐amide)/MWCNT nanocomposite films containing 2,7‐bis(4‐aminophenoxy)naphthalene and isophthalic segments
Authors: Mirsamiei Azizollah , Faghihi Khalil 
Published: February 2018

Three nanocomposite films based on aramid (poly (ether‐amide), PEA) and multiwall carbon nanotubes (MWCNT) were prepared via solution casting method using 2,7‐bis(4‐aminophenoxy)naphthalene (4) and isophthalic acid (5) containing various amounts of MWCNT (2, 3, 5 wt.%). To comprehensively analyze the properties of the cast films as well as the monomers, different techniques were employed, namely FT‐IR, 1H NMR, X‐ray diffraction, and field emission scanning electron microscopy. Also, thermal and tensile properties of PEA (6) and nanocomposite films were investigated using thermogravimetric analysis and mechanical analysis, respectively. The morphology, thermal, and mechanical properties of nanocomposite films approved that MWCNT had well dispersion in the PEA matrix and showed a synergistic effect on improving all of the investigated properties. Based on the thermogravimetric analysis results, employing MWCNT caused to increase in the char yields from 61 (in the neat PEA) to 66 (in the PEA /MWCNT nanocomposite 5 wt.%) under the nitrogen atmosphere. In comparison to the pristine PEA (426°C), the temperature at 10 losses mass % (T10) was increased from 530°C to 576°C, with 2 to 5 wt.% of MWCNT. Mechanical analysis revealed that the tensile strength and initial modulus were improved by incorporating MWCNT into PEA (81.70–93.40 MPa and 2.10–2.22 GPa, respectively). Electrical conductivity of the PEA/MWCNT nanocomposites was displayed maximum value in the 5 wt.%, showing satisfactory value in many application areas. The X‐ray diffraction technique was employed to study the crystalline structure of the prepared nanocomposite films as well as PEA. In addition, the electrochemical impedance spectroscopy study demonstrated that the prepared nanocomposites had significant impedance improvement in the presence of MWCNTs.