bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH PROGRAM STAND UP COMEDY SHOW DI METRO TV TERHADAP AKTUALISASI DIRI PENONTON (Studi Kasus Pengaruh Tayangan Stand up Comedy Show di Metro TV Terhadap Aktualisasi Diri Penonton di Café Coffee Toffee, Kalimalang)
Authors: Buana Ayu Tirta , Asmara D.A Yudha 
Published: September 2024

This study was conducted to determine the effect of Program Stand up Comedy Show on Metro TV Audience Against Self-actualization (Case Study Effect of Impression Stand up Comedy Show on Metro TV Against Self Actualization Attendance at Café Coffee Toffee, Kalimalang). In this study, researchers used a hypodermic needle theory and the theory of Abraham Maslow's hierarchy of needs is self-actualization needs. This study uses a quantitative approach, in which the results obtained in the form of data. The population consists of 50 people, with a sample of 25 people. The method used was purposive sampling the researcher. Not through a random sample techniques, in which each member of the population has a chance of being sampled, based on the characteristics that made the researcher. Research data collection techniques using primary data (obtained from the study site) and secondary data (literature study). Data were analyzed using SPSS for windows 21. 00. The data is then through the stages of editing, coding and tabulation. The study was conducted in order to find the effect of the program stand up comedy show on Metro Tv audience to self-actualization. Results of this study indicate that there are significant between stand up comedy show program on Metro TV audience against self- actualization
Designing a Model of English for Tourism Materials Using Communicative Approach at English Department of Sultan Ageng Tirtayasa University
Authors: Evenddy Sutrisno Sadji , Prabowo Jumbuh , Rima Rosmania 
Published: September 2024

The present study aims to determine and propose a model of material for English for Tourism using communicative approach. The present study uses a research and development methodology (R&D). The data collecting techniques are documentation, questionnaires, and interviews. The data gathered from documentation and interviews were analysed using content analysis while the data obtained from the questionnaires were analysed quantitatively using SPSS. The results of data analysis indicate that (1) the materials used of English for Tourism are based on lecturer’s assumptions, (2) based on the expert validation, the model material of English for Tourism falls into a very feasible category with the percentage of 86.76 %. The teaching materials based on the results of the students responses by the percentage is in the category of good (78.9 %). Based on the validation both from expert and students it could be concluded that a model of EFT material using communicative approach seems to be feasible to be applied in English Department of Sultan Ageng Tirtayasa University.
THE CORRELATION BETWEEN THE USE OF GOOGLE CLASSROOM AND ENGLISH LEARNING OUTCOMES
Authors: Azmi Dhila Nurul , Amirrachman R Alpha , Evenddy Sutrisno Sadji 
Published: September 2024

The present study aims to investigate the relationship between the use of google classroom and English learning outcomes and describe the use the google classroom and English learning at the seventh grade of SMPN 3 Cisauk. The present study utilizes a sampling technique enclosing 184 samples from 342 populations of seventh graders. Data was collected using instrument adoptions. The applied method was a survey method with a quantitative approach and ex post facto. The result of the data analysis show that the test of normality is 0,017 <0,05. The analysis was a significant positive correlation between the two variables with a correlation value of r=0,248 and coefficient of determination r2=0,061 or the contribution to the use of Google Classroom and English learning outcomes is 6,1%. The use of Google Classroom with a mean value of 91.07 is categorized as high and learning outcomes with a mean value of 84.79 are categorized as high. The results of present study suggest that the use of Google Classroom has a positive effect on the English learning outcomes of the participants.
Pulsed focused ultrasound ablation assisted by a surface modified catheter for thrombolysis: a feasibility study
Authors: Samaddar Abhirup , Forrest M. Laird , Yang Xinmai 
Published: September 2024

Interventional procedures for the recanalization of blood vessels to treat deep vein thrombosis carry a high risk of vessel wall injuries or hemorrhaging. Focused ultrasound (FUS) has been used to non-invasively break down blood clots that occlude the vessels in both in vitro and in vivo studies. Previous studies have either used thrombolytic drugs or ultrasound contrast agents (e.g., microbubbles) in combination with FUS. Several studies have applied very high peak-negative-pressures (PNP) during FUS treatment to achieve successful thrombolysis without the use of contrast agents. In the current study, we demonstrated that cavitation activity could be significantly enhanced by placing a nitinol wire, whose surface was roughed by laser etching, in the focal region of a FUS field. We demonstrated in vitro in a mock thrombosis that the thrombolysis efficacy of a 500 kHz FUS transducer was significantly enhanced using a surface-etched nitinol wire as compared to an unetched nitinol wire, whereas FUS-alone at the same pressure level did not result in any thrombolysis. These results suggest that a surface modified nitinol catheter exposed to FUS can result in intense cavitation activities leading to enhanced thrombolysis without the use of additional pharmacological or contrast agents.
Pulling away from the trigger: the influences of purpose in life and self-affirmation on decisions to shoot
Authors: Burd Kayla A. , Burrow Anthony L. , Guyll Max 
Published: September 2024

IntroductionRecent data suggests significant racial disparities in police killings in the United States: Much research finds that Black men are killed by police officers at higher rates than White men, and many individuals killed by police have been unarmed.MethodToward addressing psychological mechanisms at play in these complicated decision contexts, the current study tested the effectiveness of two writing tasks at reducing the unjustified shooting of unarmed targets using a virtual shooting-decision platform. Participants wrote either about their sense of purpose, self-affirming values, or a control topic and then played a first-person shooter video game, which randomly presented pictures of Black and White armed and unarmed targets. Participants were instructed not to shoot unarmed targets and to shoot armed targets.ResultsResults indicated that relative to controls, writing about either purpose or self-affirming values reduced the probability of shooting unarmed targets, without negatively impacting shooting decision reaction time.
Transverse resonance technique for analysis of a symmetrical open stub in a microstrip transmission line
Authors: Rassokhina Yulia V. , Krizhanovski Vladimir G. 
Published: September 2024

Open stubs in a strip (microstrip) transmission line are one of the most common elements of planar circuits used in numerous devices in the various types of wireless systems. Therefore, the urgent problem is to develop an analyzing method for discontinuities in the form of the open stub in a microstrip transmission line at frequencies at which the high-frequency effects must be considered. In the paper, a technique of scattering characteristics calculating on a symmetrical microstrip open stub by transverse resonance method is presented. Boundary value problems for a rectangular volume resonator based on a microstrip transmission line with a symmetric open stub are solved for the three options boundary conditions in the symmetry plane and on the longitudinal boundaries. The intersection of the spectral curves obtained by the numerical solution of the “electric” and “magnetic” boundary value problems determines the minima of a reflection or transmission coefficients of fundamental wave on discontinuities. To algebraize the boundary value problems for the eigen frequencies of volume resonator with discontinuity, the corresponding two-dimensional functions of the magnetic potential are constructed, through which the components of the current density on the strip are determined. The functions of magnetic potential were defined by decomposing them into expansion by Fourier series, which ensures stable convergence of the series and numerical calculation algorithm. The developed technique has been tested by calculating the eigenfrequency spectra of an open microstrip stub using the transverse resonance method on the example of an open stub in a microstrip transmission line with a resonant frequency of about 3.0 GHz. Also, a technique for numerical solutions of “electric” and “magnetic” boundary-value problems for resonators with two electrodynamically coupled symmetric open stubs in a microstrip transmission line is developed.
Low brain-derived neurotrophic factor and high vascular cell adhesion molecule-1 levels are associated with chronic kidney disease in patients with type 2 diabetes mellitus
Authors: Chiang Yu-Hsin , Li Yu-Hsuan , Chan Yin-Ching , Cheng Yu-Cheng , Wu Junyi , Lin Jer-An , Huang Wei-Chang , Lee I-Te 
Published: September 2024

BackgroundPatients with type 2 diabetes mellitus (DM) have a high prevalence of chronic kidney disease (CKD). Energy imbalance and inflammation may be involved in the pathogenesis of CKD. We examined the effects of brain-derived neurotrophic factor (BDNF) and vascular cell adhesion molecule-1 (VCAM-1) on CKD in patients with type 2 DM.MethodsPatients with type 2 DM were enrolled for this cross-sectional study. Fasting serum was prepared to measure the BDNF and VCAM-1 levels. An estimated glomerular filtration rate (eGFR) &lt;60 mL/min/1.73 m2 was used as the criterion for identifying patients with CKD.ResultsOf the 548 enrolled participants, 156 had CKD. Patients with CKD exhibited significantly lower BDNF (median of 21.4 ng/mL, interquartile range [IQR]: 17.0–27.0 ng/mL vs. median of 25.9 ng/mL, IQR: 21.0–30.4 ng/mL, P &lt;0.001) and higher VCAM-1 (median of 917 ng/mL, IQR: 761–1172 ng/mL vs. median of 669 ng/mL, IQR: 552–857 ng/mL, P &lt;0.001) levels than those without CKD. Serum BDNF levels were inversely correlated with VCAM-1 levels (Spearman’s rank correlation coefficient = -0.210, P &lt;0.001). The patients were divided into four subgroups based on median BDNF and VCAM-1 levels (24.88 ng/mL and 750 ng/mL, respectively). Notably, patients in the high VCAM-1 and low BDNF group had the highest prevalence (50%) of CKD. Multivariate logistic regression revealed a significantly higher odds ratio (OR) of CKD in the high VCAM-1 and low BDNF group (OR = 3.885, 95% CI: 1.766–8.547, P &lt;0.001), followed by that in the high VCAM-1 and high BDNF group (OR = 3.099, 95% CI: 1.373–6.992, P =0.006) compared with that in the low VCAM-1 and high BDNF group. However, the risk of CKD in the low VCAM-1 and low BDNF group was not significantly different from that in the low VCAM-1 and high BDNF group (P =0.266).ConclusionCKD in patients with type 2 DM is associated with low serum BDNF and high VCAM-1 levels. BDNF and VCAM-1 have a synergistic effect on CKD. Thus, BDNF and VCAM-1 can be potential biomarkers for CKD risk stratification in patients with type 2 DM.
Steroidogenic differentiation of human amniotic membrane-derived mesenchymal stem cells into a progesterone-/androgen-producing cell lineage by SF-1 and an estrogen-producing cell lineage by WT1−KTS
Authors: Miyazaki Yumiko , Orisaka Makoto , Fujita Yuko , Mizutani Tetsuya , Yazawa Takashi , Yoshida Yoshio 
Published: September 2024

BackgroundSex steroid hormones, primarily synthesized by gonadal somatic cells, are pivotal for sexual development and reproduction. Mice studies have shown that two transcription factors, steroidogenic factor 1 (SF-1) and Wilms’ tumor 1 (WT1), are involved in gonadal development. However, their role in human gonadal somatic differentiation remains unclear. We therefore aimed to investigate the roles of SF-1 and WT1 in human gonadal steroidogenic cell differentiation.MethodsUsing a transient lentivirus-mediated gene expression system, we assessed the effects of SF-1 and WT1 expression on the steroidogenic potential of human amniotic membrane-derived mesenchymal stem cells (hAmMSCs).ResultsSF-1 and WT1−KTS, a splice variant of WT1, played distinct roles in human steroidogenic differentiation of hAmMSCs. SF-1 induced hAmMSC differentiation into progesterone- and androgen-producing cell lineages, whereas WT1−KTS promoted hAmMSC differentiation into estrogen-producing cell lineages.ConclusionOur findings revealed that SF-1 and WT1−KTS play important roles in human gonadal steroidogenic cell differentiation, especially during ovarian development. These findings may pave the way for future studies on human ovarian differentiation and development.
Influence of sex and functional status on the value of serum steroid profiling in discriminating adrenocortical carcinoma from adrenocortical adenoma
Authors: Weng Yan , Tang Ju-Ying , Zhang Xiao-Yun , Lin Diao-Zhu , Guo Ying , Liang Ying , Wang Lin , Zhou Jing , Yan Li , Lin Tian-Xin , Zhang Shao-Ling 
Published: September 2024

BackgroundIt is challenging for clinicians to distinguish adrenocortical carcinoma (ACC) from benign adrenocortical adenomas (ACA) in their early stages. This study explored the value of serum steroid profiling as a complementary biomarker for malignancy diagnosis of ACC other than diameter and explored the influence of sex and functional status.MethodsIn this retrospective study, a matched cohort of patients diagnosed with either ACC or ACA based on histopathology was meticulously paired in a 1:1 ratio according to sex, age, and functional status. Eight serum steroids including 11-deoxycortisol, 11-deoxycorticosterone, progesterone, androstenedione, dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), 17-hydroxyprogesterone, and estradiol, were quantified by liquid chromatography tandem mass spectrometry. We conducted a comparative analysis of the clinical characteristics and serum steroid profiles of patients with ACC and ACA, with further subgroup analysis.ResultsThe study included 31 patients with ACC and 31 matched patients with ACA. Patients with ACC exhibited significantly larger tumor diameters, lower body mass index (BMI), and higher levels of 11-deoxycortisol, progesterone, and androstenedione than those with ACA. 11-deoxycortisol was the only valuable index for discriminating ACC from ACA, regardless of functional status and sex. Progesterone, DHEA, and DHEAS levels were higher in the functional ACC group than in the non-functional ACC group. Female ACC patients, especially in postmenopausal female exhibited higher levels of androstenedione than male patients. The area under the curve of tumor diameter, 11-deoxycortisol, and BMI was 0.947 (95% CI 0.889–1.000), with a sensitivity of 96.8% and specificity of 90.3%.ConclusionSerum steroid profiling serves as a helpful discriminative marker for ACC and ACA, with 11-deoxycortisol being the most valuable marker. For other steroid hormones, consideration of sex differences and functional status is crucial.
Bidirectional two-sample Mendelian randomization analysis investigates causal associations between cathepsins and inflammatory bowel disease
Authors: Wang Na , Liu Jun , Chai Bao , Yao Jianhong , Du Xufang , Mei Qi , Wang Xuena 
Published: September 2024

BackgroundCathepsins, key regulators of the pathology of gastrointestinal disorders such as inflammatory bowel disease (IBD), are a target protease that has attracted much attention in recent years. IBD is a chronic and relapsing inflammatory disorder of the gut. Traditional studies have shown a correlation between cathepsin and the risk of IBD, while the causal relationship remains unclear.MethodsThis study utilized Mendelian randomization techniques to evaluate the causal relationships between eleven cathepsins and the subtypes of IBD, such as ulcerative colitis (UC) and Crohn’s disease (CD). We also performed a series of sensitivity analyses to validate the primary Mendelian randomization (MR) results, including Cochran’s Q test, the MR-PRESSO global test, and the MR pleiotropy test.ResultsThe forward MR analyses showed no significant association between cathepsins and IBD. Reverse Mendelian randomization analyses suggested that UC might lead to elevated cathepsin G levels [inverse-variance weighted (IVW): p = 0.038, b = 9.966], and CD might cause a decrease in cathepsin B levels [IVW: p = 0.002, b = −10.525] and cathepsin L1 levels [IVW: p = 0.045, b = −4.742].ConclusionsOur findings offer novel and comprehensive evidence on the impact of UC or CD on cathepsins, potentially providing valuable insights into the treatment and prognosis of IBD.
Monozygotic triplets with juvenile-onset autoimmunity and 18p microdeletion involving PTPRM
Authors: Herlin Morten Krogh , Bernth Jensen Jens Magnus , Andreasen Lotte , Petersen Mikkel Steen , Lønskov Jonas , Thorup Mette Bendixen , Birkebæk Niels , Mogensen Trine H. , Herlin Troels , Deleuran Bent 
Published: September 2024

Abnormal gene dosage from copy number variants has been associated with susceptibility to autoimmune disease. This includes 18p deletion syndrome, a chromosomal disorder with an estimated prevalence of 1 in 50,000 characterized by intellectual disability, facial dysmorphology, and brain abnormalities. The underlying causes for autoimmune manifestations associated with 18p deletions, however, remain unknown. Our objective was to investigate a distinctive case involving monozygotic triplets concordant for developmental delay, white matter abnormalities, and autoimmunity, specifically juvenile-onset Graves’ thyroiditis. By chromosomal microarray analysis and whole genome sequencing, we found the triplets to carry a de novo interstitial 5.9 Mb deletion of chromosome 18p11.31p11.21 spanning 19 protein-coding genes. We conducted a literature review to pinpoint genes affected by the deletion that could be associated with immune dysregulation and identified PTPRM as a potential candidate. Through dephosphorylation, PTPRM serves as a negative regulator of STAT3, a key factor in the generation of Th17 cells and the onset of specific autoimmune manifestations. We hypothesized that PTPRM hemizygosity results in increased STAT3 activation. We therefore performed assays investigating PTPRM expression, STAT3 phosphorylation, Th1/Th2/Th17 cell fractions, Treg cells, and overall immunophenotype, and in support of the hypothesis, our investigations showed an increase in cells with phosphorylated STAT3 and higher levels of Th17 cells in the triplets. We propose that PTPRM hemizygosity can serve as a contributing factor to autoimmune susceptibility in 18p deletion syndrome. If confirmed in unrelated 18p/PTPRM deletion patients, this susceptibility could potentially be treated by targeted inhibition of IL-17.
Tonsil explants as a human in vitro model to study vaccine responses
Authors: Bonaiti Elena , Muraro Manuele G. , Robert Philippe A. , Jakscha Jens , Dirnhofer Stefan , Martin Ivan , Berger Christoph T. 
Published: September 2024

IntroductionVaccination is one of the most effective infection prevention strategies. Viruses with high mutation rates -such as influenza- escape vaccine-induced immunity and represent significant challenges to vaccine design. Influenza vaccine strain selection is based on circulating strains and immunogenicity testing in animal models with limited predictive outcomes for vaccine effectiveness in humans.MethodsWe developed a human in vitro vaccination model using human tonsil tissue explants cultured in 3D perfusion bioreactors to be utilized as a platform to test and improve vaccines.ResultsTonsils cultured in bioreactors showed higher viability, metabolic activity, and more robust immune responses than those in static cultures. The in vitro vaccination system responded to various premanufactured vaccines, protein antigens, and antigen combinations. In particular, a multivalent in vitro immunization with three phylogenetically distant H3N2 influenza strains showed evidence for broader B cell activation and induced higher antibody cross-reactivity than combinations with more related strains. Moreover, we demonstrate the capacity of our in vitro model to generate de novo humoral immune responses to a model antigen.DiscussionPerfusion-cultured tonsil tissue may be a valuable human in vitro model for immunology research with potential application in vaccine candidate selection.
Efficacy and safety of first-line treatments for advanced hepatocellular carcinoma patients: a systematic review and network meta-analysis
Authors: Li Jingyi , Yang Bowen , Teng Zan , Liu Yunpeng , Li Danni , Qu Xiujuan 
Published: September 2024

BackgroundThe first-line treatment for advanced hepatocellular carcinoma has evolved significantly. This study aimed to identify the most beneficial regimen.MethodsA systematic search was conducted from July 2012 to August 2024 across the following four databases: PubMed, Embase, Cochrane Library, and ClinicalTrials.gov. This search focused on phase III prospective randomized controlled trials that compared first-line treatment for advanced hepatocellular carcinoma.ResultsSeventeen studies involving 10322 patients were included in this network meta-analysis. Of the studies we included, twelve studies were global multicenter clinical studies, four were initiated in China, and one was initiated in Korea. The results of our statistical analysis suggest that Hepatic artery infusion chemotherapy with oxaliplatin plus fluorouracil (HAIC-FO) demonstrated significant overall survival (OS) benefits compared with most treatments, including various immune checkpoint inhibitors (ICIs) and anti-vascular endothelial growth factor tyrosine kinase inhibitors (VEGF-TKIs). In terms of OS, HAIC had shown similar efficacy with sorafenib plus FOLFOX (HR, 0.88; 95% CI: 0.37-2.09) and transcatheter arterial chemoembolization (TACE) combined with lenvatinib (HR, 0.69; 95% CI: 0.30-1.56). Notably, immune-related treatments, such as ICIs combined with anti-VEGF therapies, also showed improved OS compared with anti-VEGF-TKIs alone. In terms of progression-free survival (PFS), HAIC-FO outperformed anti-VEGF-TKI monotherapy, ICI monotherapy, and several ICI combinations. However, it was not superior to lenvatinib plus TACE or lenvatinib plus pembrolizumab. Based on the Surface Under the Cumulative Ranking Curve (SUCRA) values, HAIC-FO was ranked the most effective in terms of OS (SUCRA = 0.961) and objective response rate (ORR) (SUCRA = 0.971). The results of the subgroup analysis suggested that HAIC-FO achieved the best OS benefit in the macrovascular invasion (MVI) and extrahepatic spread (EHS) subgroup (SUCRA = 0.99) and that tremelimumab combined with durvalumab achieved the best OS benefit in the Asian subgroup (SUCRA = 0.88).ConclusionThis systematic review and network meta-analysis suggest that HAIC-based therapies may become a potential first-line treatment option for advanced HCC, especially for patients in Mainland China with MVI and EHS. Additionally, immune-related treatments may be more suitable for Asian populations.