bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heart-Rate Variability During Deep Sleep in World-Class Alpine Skiers: A Time-Efficient Alternative to Morning Supine Measurements
Authors: Herzig David , Testorelli Moreno , Olstad Daniela Schäfer , Erlacher Daniel , Achermann Peter , Eser Prisca , Wilhelm Matthias 
Published: September 2016

Background:It is increasingly popular to use heart-rate variability (HRV) to tailor training for athletes. A time-efficient method is HRV assessment during deep sleep.Aim:To validate the selection of deep-sleep segments identified by RR intervals with simultaneous electroencephalography (EEG) recordings and to compare HRV parameters of these segments with those of standard morning supine measurements.Methods:In 11 world-class alpine skiers, RR intervals were monitored during 10 nights, and simultaneous EEGs were recorded during 2–4 nights. Deep sleep was determined from the HRV signal and verified by delta power from the EEG recordings. Four further segments were chosen for HRV determination, namely, a 4-h segment from midnight to 4 AM and three 5-min segments: 1 just before awakening, 1 after waking in supine position, and 1 in standing after orthostatic challenge. Training load was recorded every day.Results:A total of 80 night and 68 morning measurements of 9 athletes were analyzed. Good correspondence between the phases selected by RR intervals vs those selected by EEG was found. Concerning root-mean-squared difference of successive RR intervals (RMSSD), a marker for parasympathetic activity, the best relationship with the morning supine measurement was found in deep sleep.Conclusion:HRV is a simple tool for approximating deep-sleep phases, and HRV measurement during deep sleep could provide a time-efficient alternative to HRV in supine position.
The Impact of Central and Peripheral Cyclooxygenase Enzyme Inhibition on Exercise-Induced Elevations in Core Body Temperature
Authors: Veltmeijer Matthijs T.W. , Veeneman Dineke , Bongers Coen C.C.W. , Netea Mihai G. , van der Meer Jos W. , Eijsvogels Thijs M.H. , Hopman Maria T.E. 
Published: September 2016

Purpose:Exercise increases core body temperature (TC) due to metabolic heat production. However, the exercise-induced release of inflammatory cytokines including interleukin-6 (IL-6) may also contribute to the rise in TC by increasing the hypothalamic temperature set point. This study investigated whether the exercise-induced increase in TC is partly caused by an altered hypothalamic temperature set point.Methods:Fifteen healthy, active men age 36 ± 14 y were recruited. Subjects performed submaximal treadmill exercise in 3 randomized test conditions: (1) 400 mg ibuprofen and 1000 mg acetaminophen (IBU/APAP), (2) 1000 mg acetaminophen (APAP), and (3) a control condition (CTRL). Acetaminophen and ibuprofen were used to block the effect of IL-6 at a central and peripheral level, respectively. TC, skin temperature, and heart rate were measured continuously during the submaximal exercise tests.Results:Baseline values of TC, skin temperature, and heart rate did not differ across conditions. Serum IL-6 concentrations increased in all 3 conditions. A significantly lower peak TC was observed in IBU/APAP (38.8°C ± 0.4°C) vs CTRL (39.2°C ± 0.5°C, P = .02) but not in APAP (38.9°C ± 0.4°C) vs CTRL. Similarly, a lower ΔTC was observed in IBU/APAP (1.7°C ± 0.3°C) vs CTRL (2.0°C ± 0.5°C, P < .02) but not in APAP (1.7°C ± 0.5°C) vs CTRL. No differences were observed in skin temperature and heart-rate responses across conditions.Conclusions:The combined administration of acetaminophen and ibuprofen resulted in an attenuated increase in TC during exercise compared with a CTRL. This observation suggests that a prostaglandin-E2-induced elevated hypothalamic temperature set point may contribute to the exercise-induced rise in TC.
The Use of Recovery Strategies Among Participants of the BUPA Great North Run: A Cross-Sectional Survey
Authors: Shaw Sarah , Smith Tina , Alexanders Jenny , Shaw Thomas , Smith Lois , Nevill Alan , Anderson Anna 
Published: September 2016

Objective:To investigate half-marathon runners’ frequency of use of recovery strategies, perceptions regarding the most beneficial recovery strategy, and reasons for using recovery strategies.Design:Cross-sectional survey.Participants:186 participants of the 13.1 mile BUPA Great North Run 2013.Methods:A questionnaire was developed which required participants to indicate how frequently they used 12 different recovery strategies, identify which recovery strategy they believed to be most beneficial, and rank 6 reasons for using recovery strategies in order of importance. Data were analyzed using a Friedman nonparametric ANOVA and additional nonparametric tests.Results:All participants used recovery strategies. Stretching was the most commonly used recovery strategy (P < .001), whereas the use of nutritional supplements was the most commonly selected most beneficial recovery strategy. More than 50% of respondents indicated that they never used strategies such as kinesio tape (80%), hydrotherapy (78%), or ice baths (71%). A significant difference was observed between reasons for using recovery strategy (χ2 (5) = 292.29, P < .001). Reducing muscle tightness (rank 4.87) and reducing injury (rank 4.35) were the most frequently chosen most important reasons for using recovery strategies. Minor sex and age differences in the responses were identified.Conclusion:Recovery strategy usage appears to be widespread among half-marathon runners; however, disparities exist between the frequency of use and perceived effectiveness of different recovery strategies. Further research in this area is needed to facilitate the development of recovery strategy guidelines which are both evidence-based and practically relevant.
Whole-Genome Sequencing Identifies
In Vivo
Acquisition of a
bla
CTX-M-27
-Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection
Authors: McCollister Bruce , Kotter Cassandra V. , Frank Daniel N. , Washburn Taylor , Jobling Michael G. 
Published: September 2016

ABSTRACT

We report a case of ceftriaxone treatment failure for bacteremia caused by
Salmonella enterica
subsp.
enterica
serovar Typhimurium, due to the
in vivo
acquisition of a
bla
CTX-M-27
-encoding IncFII group transmissible plasmid. The original β-lactamase-susceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to
Escherichia coli
at 37°C. We used whole-genome sequencing of ST882S and ST931R, the
E. coli
transconjugant, and isolated plasmid DNA to unequivocally show that ST882S and ST931R had identical chromosomes, both having 206 identical single-nucleotide polymorphisms (SNPs) versus
S
. Typhimurium 14028s. We assembled a complete circular genome for ST931R, to which ST882S reads mapped with no SNPs. ST882S and ST931R were isogenic except for the presence of three additional plasmids in ST931R. ST931R and the
E. coli
transconjugant were ceftriaxone resistant due to the presence of a 60.5-kb IS
26
-flanked,
bla
CTX-M-27
-encoding IncFII plasmid. Compared to 14082s, ST931R has almost identical Gifsy-1, Gifsy-2, and ST64B prophages, lacks Gifsy-3, and instead carries a unique Fels-2 prophage related to that found in LT2. ST882S and ST931R both had a 94-kb virulence plasmid showing >99% identity with pSLT14028s and a cryptic 3,904-bp replicon; ST931R also has cryptic 93-kb IncI1 and 62-kb IncI2 group plasmids. To the best of our knowledge,
in vivo
acquisition of extended-spectrum β-lactamase resistance by
S
. Typhimurium and
bla
CTX-M-27
genes in U.S. isolates of
Salmonella
have not previously been reported.
Acraspisoides gen. nov. (Diptera: Therevidae: Agapophytinae): a new genus of stiletto-flies from Australia
Authors: HILL HILARY N. , WINTERTON SHAUN L. 
Published: September 2016

A new Australian genus of Therevidae, Acraspisoides gen. nov., comprising a single species (A. helviarta sp. nov.) is described and illustrated. This new genus is placed within the subfamily Agapophytinae based on the presence of velutum patches on the fore and hind femora. Acraspisoides is easily separated from other agapophytine genera by the combination of characters: large ventral lobe on aedeagus, multiple rows of postocular setae in both sexes, antennae positioned low on frons, and wing cell m 3 closed. Cladistic analyses using all genera of Agapophytinae (including Acraspisoides) based on adult morphological characters and sequence data of the protein-encoding gene, elongation factor-1 (EF-1a ), were performed to determine the phylogenetic placement of Acraspisoides gen. nov. in the subfamily. Analysis of the combined morphological and molecular matrices produced two most parsimonious trees, placing Acraspisoides gen. nov. as the most basal genus of Agapophytinae.
Precipitation over Monsoon Asia: A Comparison of Reanalyses and Observations
Authors: Ceglar Andrej , Toreti Andrea , Balsamo Gianpaolo , Kobayashi Shinya 
Published: September 2016

AbstractReanalysis products represent a valuable source of information for different impact modeling and monitoring activities over regions with sparse observational data. It is therefore essential to evaluate their behavior and their intrinsic uncertainties. This study focuses on precipitation over monsoon Asia, a key agricultural region of the world. Four reanalysis datasets are evaluated, namely ERA-Interim, ERA-Interim/Land, AgMERRA (an agricultural version of MERRA), and JRA-55. APHRODITE and the Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) dataset are the two gridded observational datasets used for the evaluation; the former is based on rain gauge data and the latter on a combination of satellite and rain gauge data. Differences in seasonality, moderate-to-heavy precipitation events, daily distribution, and drought characteristics are analyzed. Results show remarkable differences between the APHRODITE and CHIRPS observational datasets as well as between these datasets and the reanalyses. AgMERRA generally achieves the best performance, but it is not updated at near–real time. ERA-Interim/Land shows good spatial performance, but when the interest is on the temporal evolution JRA-55 is recommended, as it exhibits the most stable temporal behavior. This study shows that the use of reanalyses for impact modeling and monitoring over monsoon Asia requires an accurate evaluation and choices to be tailored to the specific needs.
Roles of Remote and Local Forcings in the Variation and Prediction of Regional Maritime Continent Rainfall in Wet and Dry Seasons
Authors: Zhang Tuantuan , Yang Song , Jiang Xingwen , Huang Bohua 
Published: September 2016

Abstract
Seasonal prediction of extratropical climate (e.g., the East Asian climate) is partly dependent upon the prediction skill for rainfall over the Maritime Continent (MC). A previous study by the authors found that the NCEP Climate Forecast System, version 2 (CFSv2), had difference in skill between predicting rainfall over the western MC (WMC) and the eastern MC (EMC), especially in the wet season. In this study, the potential mechanisms for this phenomenon are examined. It is shown that observationally in the wet season (from boreal winter to early spring) the EMC rainfall is closely linked to both ENSO and local sea surface temperature (SST) anomalies, whereas the WMC rainfall is only moderately correlated with ENSO. The model hindcast unrealistically predicts the relationship of the WMC rainfall with local SST and ENSO (even opposite to the observed feature), which contributes to lower prediction skill for the WMC rainfall. In the dry season (from boreal late summer to fall), the rainfall over the entire MC is significantly influenced by both ENSO and local SST in observations and this feature is well captured by the CFSv2. Therefore, the hindcasts do not show apparently different skill in rainfall prediction for EMC and WMC in the dry season. The possible roles of atmospheric internal processes are also discussed.
Respiratory infection rates differ between geographically distant paediatric cystic fibrosis cohorts
Authors: Ramsey Kathryn A. , Hart Emily , Turkovic Lidija , Padros-Goossens Marc , Stick Stephen M. , Ranganathan Sarath C. 
Published: September 2016

Respiratory infections are a major cause of pulmonary decline in children with cystic fibrosis (CF). We compared the prevalence of infection in early life at geographically distant CF treatment centres participating in the same surveillance programme in Australia.Lower airway microbiology, inflammation and structural lung disease at annual review were evaluated for 260 children 0–8 years old with CF at 1032 visits to CF treatment centres in Melbourne or Perth.Melbourne patients were more likely to be culture-positive for common respiratory pathogens at all age groups (odds ratio (OR) 1.85, 95% CI 1.33–2.58). Subjects <2 years old in Melbourne were also more likely to have neutrophil elastase present (OR 3.11, 95% CI 1.62–5.95). Bronchiectasis (OR 2.02, 95% CI 1.21–3.38) and air trapping (OR 2.53, 95% CI 1.42–4.51) in subjects 2–5 years old was more common in Melbourne subjects. The severity of structural lung disease was also worse in Melbourne patients >5 years old. Patients at both centres had a similar rate of hospitalisations and prescribed antibiotics.No procedural differences were identified that could explain the disparity between pathogen prevalence. Geographical differences in early acquisition of infection may contribute to variability in outcomes between CF centres.
Information systems for mental health in six low and middle income countries: cross country situation analysis
Authors: Upadhaya Nawaraj , Jordans Mark J. D. , Abdulmalik Jibril , Ahuja Shalini , Alem Atalay , Hanlon Charlotte , Kigozi Fred , Kizza Dorothy , Lund Crick , Semrau Maya , Shidhaye Rahul , Thornicroft Graham , Komproe Ivan H. , Gureje Oye 
Published: September 2016

Abstract
Background
Research on information systems for mental health in low and middle income countries (LMICs) is scarce. As a result, there is a lack of reliable information on mental health service needs, treatment coverage and the quality of services provided.

Methods
With the aim of informing the development and implementation of a mental health information sub-system that includes reliable and measurable indicators on mental health within the Health Management Information Systems (HMIS), a cross-country situation analysis of HMIS was conducted in six LMICs (Ethiopia, India, Nepal, Nigeria, South Africa and Uganda), participating in the ‘Emerging mental health systems in low and middle income countries’ (Emerald) research programme. A situation analysis tool was developed to obtain and chart information from documents in the public domain. In circumstances when information was inadequate, key government officials were contacted to verify the data collected. In this paper we compare the baseline policy context, human resources situation as well as the processes and mechanisms of collecting, verifying, reporting and disseminating mental health related HMIS data.

Results
The findings suggest that countries face substantial policy, human resource and health governance challenges for mental health HMIS, many of which are common across sites. In particular, the specific policies and plans for the governance and implementation of mental health data collection, reporting and dissemination are absent. Across sites there is inadequate infrastructure, few HMIS experts, and inadequate technical support and supervision to junior staff, particularly in the area of mental health. Nonetheless there are also strengths in existing HMIS where a few mental health morbidity, mortality, and system level indicators are collected and reported.

Conclusions
Our study indicates the need for greater technical and resources input to strengthen routine HMIS and develop standardized HMIS indicators for mental health, focusing in particular on indicators of coverage and quality to facilitate the implementation of the WHO mental health action plan 2013–2020.
RETRACTED ARTICLE: TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways
Authors: Yang Run-Wei , Zeng Ying-Yue , Wei Wen-Ting , Cui Yan-Mei , Sun Hui-Ying , Cai Yue-Long , Nian Xin-Xin , Hu Yun-Teng , Quan Yu-Ping , Jiang Sheng-Lu , Wang Meng , Zhao Ya-Li , Qiu Jun-Feng , Li Ming-Xuan , Zhang Jia-Huan , He Mei-Rong , Liang Li , Ding Yan-Qing , Liao Wen-Ting 
Published: September 2016

Abstract
Background
Transducin-like enhancer of Split3 (TLE3) serves as a transcriptional corepressor during cell differentiation and shows multiple roles in different kinds of cancers. Recently, TLE3 together with many other genes involved in Wnt/β-catenin pathway were detected hyper-methylated in colorectal cancer (CRC). However, the potential role and the underlying mechanism of TLE3 in CRC progression remain scarce.

Methods
Gene expression profiles were analyzed in The Cancer Genome Atlas (TCGA) microarray dataset of 41 normal colorectal intestine tissues and 465 CRC tissues. Western blot and Real-time Quantitative PCR (RT-qPCR) were respectively performed to detect protein and mRNA expression in 8 pairs of CRC tissue and matched adjacent normal mucosa. Immunohistochemistry (IHC) was conducted to evaluate TLE3 protein expression in 105 paraffin-embedded, archived human CRC tissues from patients, whose survival data were analyzed with Kaplan-Meier method. In vitro experiments including MTT assay, colony formation assay, and soft agar formation assay were used to investigate the effects of TLE3 on CRC cell growth and proliferation. Additionally, subcutaneous tumorigenesis assay was performed in nude mice to confirm the effects of TLE3 in vivo. Furthermore, gene set enrichment analysis (GSEA) was run to explore potential mechanism of TLE3 in CRC, and then we measured the distribution of CRC cell cycle phases and apoptosis by flow cytometry, as well as the impacts of TLE3 on MAPK and AKT signaling pathways by Western blot and RT-qPCR.

Results
TLE3 was significantly down-regulated in 465 CRC tissues compared with 41 normal tissues. Both protein and mRNA expressions of TLE3 were down-regulated in CRC compared with matched adjacent normal mucosa. Lower expression of TLE3 was significantly associated with poorer survival of patients with CRC. Besides, knock down of TLE3 promoted CRC cell growth and proliferation, while overexpression of TLE3 showed suppressive effects. Furthermore, overexpression of TLE3 caused G1-S phase transition arrest, inhibition of MAPK and AKT pathways, and up-regulation of p21Cip1/WAF1 and p27Kip1.

Conclusion
This study indicated that TLE3 repressed CRC proliferation partly through inhibition of MAPK and AKT signaling pathways, suggesting the possibility of TLE3 as a biomarker for CRC prognosis.
Evaluation of a channel assignment scheme in mobile network systems
Authors: Nurelmadina Nahla , Nafea Ibtehal , Younas Muhammad 
Published: September 2016

AbstractThe channel assignment problem is a complex problem which requires that under certain constraints a minimum number of channels have to be assigned to mobile calls in the wireless mobile system. In this paper, we propose a new scheme, which is based on double band frequency and channel borrowing strategy. The proposed scheme takes into account factors such as limited bandwidth of wireless networks and the capacity of underlying servers involved in processing mobile calls. It aims to ensure end-to-end performance by considering the characteristics of mobile devices. This is achieved by determining the position of users (or mobile stations) in wireless mobile systems. The proposed scheme is simulated in order to investigate its efficiency within a specific area of a large city in Saudi Arabia. Experimental results demonstrate that the proposed scheme significantly improves the performance of mobile calls as well as reduces the blocking when the number of mobile call increases.
Stable geographic forwarding with link lifetime prediction in mobile adhoc networks for battlefield environment
Authors: Jaiswal Priyanka , Sinha Adwitiya 
Published: September 2016

AbstractMobile adhoc network (MANET) is one of the most relevant areas of research in wireless communication that has gained prevalence due to its diversity over large-scale highly mobile networks to small-scale networks having low mobility and power constraints. Adhoc networks are inherently envisioned to be highly vulnerable to dynamic changes because of mobile sensor nodes. Node mobility often results in breakage of communication links, thereby introducing additional overhead for establishing new routes and transmitting table updates, further causing rapid exhaustion of energy reserve. Especially in military applications, preventing communication disruptions is an important security concern for defence applications engaged in safeguarding national boundaries. This necessitates the need for efficient routing strategy for battlefield environments susceptible to frequent link failures due to random mobility of groups/individuals. In this regard, we have proposed an efficient stable geographic forwarding with link-lifetime prediction (SGFL) that utilizes the broadcast nature of wireless channel and multicasts with node mobility. During the next hop selection process, a node preferably selects the neighbours which are at the least distance from the destination with low mobility. Unlike position based opportunistic routing, our scheme allows selection of backup node that lies within the transmission range of selected neighbours. Link lifetime prediction with backup nodes enhances efficiency and reliability of routing in highly mobile and congested adhoc networks. Simulation results show that our proposed SGFL achieves better performance than existing counterparts in terms of packet delivery ratio, packet loss and end-to-end delay under high node density as well as increased traffic flow.
Permanent draft genome of Thermithiobacillus tepidarius DSM 3134T, a moderately thermophilic, obligately chemolithoautotrophic member of the Acidithiobacillia
Authors: Boden Rich , Hutt Lee P. , Huntemann Marcel , Clum Alicia , Pillay Manoj , Palaniappan Krishnaveni , Varghese Neha , Mikhailova Natalia , Stamatis Dimitrios , Reddy Tatiparthi , Ngan Chew Yee , Daum Chris , Shapiro Nicole , Markowitz Victor , Ivanova Natalia , Woyke Tanja , Kyrpides Nikos 
Published: September 2016

AbstractThermithiobacillus tepidarius DSM 3134T was originally isolated (1983) from the waters of a sulfidic spring entering the Roman Baths (Temple of Sulis-Minerva) at Bath, United Kingdom and is an obligate chemolithoautotroph growing at the expense of reduced sulfur species. This strain has a genome size of 2,958,498 bp. Here we report the genome sequence, annotation and characteristics. The genome comprises 2,902 protein coding and 66 RNA coding genes. Genes responsible for the transaldolase variant of the Calvin-Benson-Bassham cycle were identified along with a biosynthetic horseshoe in lieu of Krebs’ cycle sensu stricto. Terminal oxidases were identified, viz. cytochrome c oxidase (cbb3, EC 1.9.3.1) and ubiquinol oxidase (bd, EC 1.10.3.10). Metalloresistance genes involved in pathways of arsenic and cadmium resistance were found. Evidence of horizontal gene transfer accounting for 5.9 % of the protein-coding genes was found, including transfer from Thiobacillus spp. and Methylococcus capsulatus Bath, isolated from the same spring. A sox gene cluster was found, similar in structure to those from other Acidithiobacillia – by comparison with Thiobacillus thioparus and Paracoccus denitrificans, an additional gene between soxA and soxB was found, annotated as a DUF302-family protein of unknown function. As the Kelly-Friedrich pathway of thiosulfate oxidation (encoded by sox) is not used in Thermithiobacillus spp., the role of the operon (if any) in this species remains unknown. We speculate that DUF302 and sox genes may have a role in periplasmic trithionate oxidation.