bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotinic receptor activation augments muscarinic receptor‐mediated eccrine sweating but not cutaneous vasodilatation in young males
Authors: Fujii Naoto , Louie Jeffrey C. , McNeely Brendan D. , Zhang Sarah Y. , Tran My‐An , Kenny Glen P. 
Published: November 2016

New Findings
What is the central question of this study?
Acetylcholine released from cholinergic nerves can activate both muscarinic and nicotinic receptors. Although each receptor can independently induce cutaneous vasodilatation and eccrine sweating, it remains to be elucidated whether the two receptors interact in order to mediate these responses.

What is the main finding and its importance?
We show that although nicotinic receptor activation does not modulate muscarinic cutaneous vasodilatation, it lowers the muscarinic receptor agonist threshold at which onset for eccrine sweating (augmentation of muscarinic eccrine sweating) occurs in young men in normothermic resting conditions. These results provide new insights into the physiological significance of nicotinic receptors in the regulation of cutaneous perfusion and eccrine sweating.

Acetylcholine released from cholinergic nerves can activate both muscarinic and nicotinic receptors; each is known independently to induce cutaneous vasodilatation and eccrine sweating in humans. However, it is not known whether the two receptors interact in order to mediate cutaneous vasodilatation and eccrine sweating. In 10 young men (27 ± 6 years old), cutaneous vascular conductance and sweat rate were evaluated at intradermal microdialysis sites that were continuously perfused with either lactated Ringer's solution (control) or three different concentrations of nicotine (0.1, 1 and 10 mm), a nicotinic receptor agonist. Co‐administration of methacholine, a muscarinic receptor agonist, was performed at all skin sites in a dose‐proportional fashion (0.0125, 0.25, 5, 100 and 2000 mm, each for 25 min). Administration of nicotine alone caused dose‐dependent transient increases in cutaneous vascular conductance and sweat rate (all P ≤ 0.05), which thereafter returned to pre‐nicotine levels, except that a portion of transient responses remained with continuous administration of 10 mm nicotine (both P ≤ 0.05). Cutaneous vascular conductance was increased by administration of ≥0.25 mm methacholine at the control site, and this response was likewise observed in the presence of co‐administration of all doses of nicotine used (all P ≤ 0.05). Sweat rate at the control site was increased by administration of ≥0.25 mm methacholine, but the lowest dose of methacholine (0.0125 mm) was able to increase sweat rate in the presence of 10 mm nicotine (P ≤ 0.05). We conclude that nicotinic receptor activation lowers the muscarinic receptor agonist threshold for eccrine sweating (augmentation of muscarinic sweating) but does not affect muscarinic cutaneous vasodilatation in young men in normothermic resting conditions.
Biological enhancement of graft-tunnel healing in anterior cruciate ligament reconstruction
Authors: Saccomanno Maristella , Capasso Luigi , Fresta Luca , Milano Giuseppe 
Published: November 2016

The sites where graft healing occurs within the bone tunnel and where the intra-articular ligamentization process takes place are the two most important sites of biological incorporation after anterior cruciate ligament (ACL) reconstruction, since they help to determine the mechanical behavior of the femur-ACL graft-tibia complex. Graft-tunnel healing is a complex process influenced by several factors, such as type of graft, preservation of remnants, bone quality, tunnel length and placement, fixation techniques and mechanical stress. in recent years, numerous experimental and clinical studies have been carried out to
evaluate potential strategies designed to enhance and optimize the biological environment of the graft-tunnel interface.Modulation of inflammation, tissue engineering and gene transfer techniques have been applied in order to obtain a direct-type fibrocartilaginous insertion of the ACL graft, similar to that of native ligament, and to accelerate the healing process of tendon grafts within the bone tunnel. Although animal studies have given encouraging results, clinical studies are lacking and their results do not really support the use of the various strategies in clinical practice. Further investigations are therefore needed to optimize delivery techniques, therapeutic concentrations, maintenance of therapeutic effects over time, and to reduce the risk of undesirable effects in clinical practice.
Semitendinosus and gracilis free muscle-tendon graft for repair of massive rotator cuff tears: surgical technique
Authors: Gigante Antonio , Bottegoni Carlo , Milano Giuseppe , Riccio Michele , Dei Giudici Luca 
Published: November 2016

Massive rotator cuff tears are difficult to treat surgically due to retraction, degeneration and fraying of the ends of torn tendons, severe fatty infiltration and atrophy of the respective muscles.Procedures developed to close the gap between the rotator cuff and the greater tuberosity of the humerus, such as soft tissue release may be inadequate for
large tears. Human or porcine dermal allografts still have uncertain benefits, and tendon transfers seem to be associated with poor outcomes, donor site morbidity and altered mechanics. Reverse total shoulder arthroplasty has limited durability and is not indicated in young patients with high functional demands. We developed a new technique for repairing massive rotator cuff tears by semitendinosus and gracilis myotendinous grafting. This novel therapeutic option allows massive rotator cuff tears to be repaired using autologous material that is adequate and adaptable, making it possible to cover any width of defect. The technique is low-invasive and not technically demanding, with minimal donor site morbidity.
Quality and Health-Optimizing Physical Education: Using Assessment at the Health and Education Nexus
Authors: Dudley Dean , Goodyear Victoria , Baxter David 
Published: November 2016

Background:The United Nations Educational, Cultural and Scientific Organization (UNESCO) recognizes quality physical education (QPE) must, along with physical, social and affective educative goals, seek to improve the health status of youth (UNESCO, 2015). Health-Optimizing Physical Education (HOPE) is a model of physical education (PE) that seeks this goal but is creating much debate in the discipline (Sallis et al., 2012).Purpose:The aim of this paper is to present a conceptual assessment framework for QPE and HOPE on which future assessment protocols may be based that serve both health and educative goals.Methods/Data analysis:Policy and literature pertaining to QPE and HOPE were reviewed and compared for similarities and differences. This was followed by an analysis of literature on assessment in the health and education disciplines. These analyses provided the authors with the insight to propose a new model of assessment for HOPE models to implement QPE.Results:Many similarities exist in the policy of QPE and the published literature on HOPE. However, the measurement model of assessment can often circumvent two important assessment functions for education settings that need to be addressed in a wider QPE and Models-Based Practice (MBP) context. Conclusions: HOPE models were established using an interventionist mindset and are therefore well suited to integrating well-defined MBP pedagogies as appropriate ‘intervening’ strategies by using a clinical approach to teaching and assessment. To date, they have lacked an assessment framework that has been capable of addressing both the health and educative goals that both HOPE and MBP seek to achieve. This paper provides new insight by reimagining the role MBPs and assessment practices have to play in the health and education nexus.
Models-Based Practice in Physical Education: The Case for Sport Education
Authors: Hastie Peter A. , Wallhead Tristan 
Published: November 2016

Purpose:This paper provides a potential roadmap for the future development of research on Sport Education. In the first part of the paper, research on each of the elements of competence, literacy and enthusiasm are reviewed, with the aim of providing evidence to support the idea that the model can achieve its goals. For each of these goals we provide some potential directions which we believe are important for moving research on Sport Education forward.Development:These avenues include more attention to appropriate practices for enhancing student-coach effectiveness, ways to enhance the development of more equitable and inclusive class environments within the model, as well as the potential transfer of Sport Education experiences to physical activity environments beyond physical education.Design:Research designs need to include how teachers and students give value and significance to what they teach and what they learn, respectively. This could be achieved through researchers considering more prolonged action-based research designs that allow a close monitoring of the implementation of pedagogical approaches. These case studies can provide guidance for future pedagogical iterations of the model that can be applied within more generalizable group designs.
Teacher Fidelity to a Physical Education Curricular Model and Physical Activity Outcomes
Authors: Stylianou Michalis , Kloeppel Tiffany , Kulinna Pamela , van der Mars Han 
Published: November 2016

Background:This study was informed by the bodies of literature emphasizing the role of physical education in promoting physical activity (PA) and addressing teacher fidelity to curricular models.Purpose:The purpose of this study was to compare student PA levels, lesson context, and teacher PA promotion behavior among classes where teachers were using the Dynamic Physical Education (DPE) curricular model with low, moderate, and high fidelity.Methods:Participants were 20 physical education teachers, and their 4th and 5th grade students. Each teacher was observed teaching three times during the study. Fidelity data were collected using a validated observation instrument. PA, lesson context, and teacher behavior data were collected using the System for Observing Fitness Instruction Time (SOFIT). Data analysis included descriptive statistics and group difference tests.Results:Significant differences among the three fidelity groups were identified in several items of the observation instrument. No significant moderate-to-vigorous PA or lesson context differences were found among the three groups. Students taught by teachers in the high fidelity group spent a significantly higher proportion of lesson time (7.5%) in vigorous PA than students taught by teachers in the low fidelity group. Teachers in the moderate and high fidelity groups spent a significantly higher proportion of lesson time promoting in-class PA than teachers in the low fidelity group.Discussion:Fidelity of implementation to the DPE model had little impact on student PA. The findings of this study can inform future researchers about the methodological importance of examining teacher fidelity to curricular models and associated outcomes.
The Possibilities of “Doing” Outdoor and/or Adventure Education in Physical Education/Teacher Education
Authors: Sutherland Sue , Legge Maureen 
Published: November 2016

Background:Physical education has a long association with teaching outdoor and/or adventure education (OAE). As physical education teacher educators, with a special interest in teaching OAE, we wanted to examine perceptions of models based practices in physical education/teacher education.Purpose:This manuscript; explores and critiques a range of national and international perspectives on models based practices in OAE; challenges what stands for teaching OAE in PETE; and offers suggestions for future practice and research. Method: Papers were selected through a systematic review methodology.Data analysis:Using a process of inductive analysis and constant comparison we identified two main themes: Ways of doing this in PE and Ways of doing this in PETE.Discussion/Conclusion:Future recommendations include the pedagogical relevance and importance of understanding the socio-cultural context, the challenge of adventure education being a controlled orchestration and the need to pedagogically change the key of this orchestration, and employing innovative methodological approaches to further explore these issues.
Chromoblastomycosis
Authors: Queiroz-Telles Flavio , de Hoog Sybren , Santos Daniel Wagner C. L. , Salgado Claudio Guedes , Vicente Vania Aparecida , Bonifaz Alexandro , Roilides Emmanuel , Xi Liyan , Azevedo Conceição de Maria Pedrozo e Silva , da Silva Moises Batista , Pana Zoe Dorothea , Colombo Arnaldo Lopes , Walsh Thomas J. 
Published: November 2016

SUMMARY
Chromoblastomycosis (CBM), also known as chromomycosis, is one of the most prevalent implantation fungal infections, being the most common of the gamut of mycoses caused by melanized or brown-pigmented fungi. CBM is mainly a tropical or subtropical disease that may affect individuals with certain risk factors around the world. The following characteristics are associated with this disease: (i) traumatic inoculation by implantation from an environmental source, leading to an initial cutaneous lesion at the inoculation site; (ii) chronic and progressive cutaneous and subcutaneous tissular involvement associated with fibrotic and granulomatous reactions associated with microabscesses and often with tissue proliferation; (iii) a nonprotective T helper type 2 (Th2) immune response with ineffective humoral involvement; and (iv) the presence of muriform (sclerotic) cells embedded in the affected tissue. CBM lesions are clinically polymorphic and are commonly misdiagnosed as various other infectious and noninfectious diseases. In its more severe clinical forms, CBM may cause an incapacity for labor due to fibrotic sequelae and also due to a series of clinical complications, and if not recognized at an early stage, this disease can be refractory to antifungal therapy.
From Q Fever to Coxiella burnetii Infection: a Paradigm Change
Authors: Eldin Carole , Mélenotte Cléa , Mediannikov Oleg , Ghigo Eric , Million Matthieu , Edouard Sophie , Mege Jean-Louis , Maurin Max , Raoult Didier 
Published: November 2016

SUMMARYCoxiella burnetiiis the agent of Q fever, or “query fever,” a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic.C. burnetiiis classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture.C. burnetiiinfects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection withC. burnetiiwas the breaking of the old dichotomy between “acute” and “chronic” Q fever. The clinical presentation ofC. burnetiiinfection depends on both the virulence of the infectingC. burnetiistrain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients withC. burnetiiinfection.
Draft Genome Sequence of Lactobacillus plantarum XJ25 Isolated from Chinese Red Wine
Authors: Zhao Meijing , Liu Shuwen , He Ling , Tian Yu 
Published: November 2016

ABSTRACT

Here, we present the draft genome sequence of
Lactobacillus plantarum
XJ25, isolated from Chinese red wine that had undergone spontaneous malolactic fermentation, which consists of 25 contigs and is 3,218,018 bp long.
Genome Sequences of Two Multidrug-Resistant Proteus mirabilis Strains Harboring CTX-M-65 Isolated from Malaysia
Authors: Yu Choo Yee , Ang Geik Yong , Ngeow Yun Fong , Tee Kok Keng , Yin Wai-Fong , Chan Kok-Gan 
Published: November 2016

ABSTRACT

Proteus mirabilis
is an opportunistic nosocomial pathogen that is commonly associated with urinary tract infections. Here, we present draft genome sequences of two multidrug-resistant
P. mirabilis
strains, isolated from urine samples in Malaysia, that harbored a CTX-M-type extended-spectrum β-lactamase-encoding gene, as well as a repertoire of other antimicrobial-resistant determinants.
Draft Genome Sequence of the Thermotolerant Cyanobacterium
Desertifilum
sp. IPPAS B-1220
Authors: Mironov Kirill S. , Sinetova Maria A. , Bolatkhan Kenzhegul , Zayadan Bolatkhan K. , Ustinova Vera V. , Kupriyanova Elena V. , Skrypnik Alexandra N. , Gogoleva Natalya E. , Gogolev Yuriy V. , Los Dmitry A. 
Published: November 2016

ABSTRACT

Here, we report the draft genome of the filamentous cyanobacterium
Desertifilum
sp. strain IPPAS B-1220, isolated from Lake Shar-Nuur, Mongolia. The genome of 6.1 Mb codes for 5,113 genes. Genome mining revealed 10 clusters for the synthesis of bioactive compounds (nonribosomal peptides, polyketides, bacteriocins, and lantipeptides) with potential biotechnological or medical importance.
Draft Genome Sequence of Pseudomonas mosselii Gil3, Isolated from Catfish and Antagonistic against Hypervirulent Aeromonas hydrophila
Authors: Zhang Dunhua , Xu De-Hai , Qiu Junqiang , Rasmussen-Ivey Cody R. , Liles Mark R. , Beck Benjamin H. 
Published: November 2016

ABSTRACT

Pseudomonas mosselii
Gil3 was isolated from a catfish that survived from lethal challenge with hypervirulent
Aeromonas hydrophila
(vAh). When assayed
in vitro
, the bacterium showed antagonism against vAh. Sequence analysis revealed that the genome of
P. mosselii
Gil3 encodes numerous aromatic metabolism pathways and proteins for biosynthesis of antimicrobial compounds.
Draft Genome Sequence of
Cyanobacterium
sp. Strain IPPAS B-1200 with a Unique Fatty Acid Composition
Authors: Starikov Alexander Y. , Usserbaeva Aizhan A. , Sinetova Maria A. , Sarsekeyeva Fariza K. , Zayadan Bolatkhan K. , Ustinova Vera V. , Kupriyanova Elena V. , Los Dmitry A. , Mironov Kirill S. 
Published: November 2016

ABSTRACT

Here, we report the draft genome of
Cyanobacterium
sp. IPPAS strain B-1200, isolated from Lake Balkhash, Kazakhstan, and characterized by the unique fatty acid composition of its membrane lipids, which are enriched with myristic and myristoleic acids. The approximate genome size is 3.4 Mb, and the predicted number of coding sequences is 3,119.
Volatile dilution during magma injections and implications for volcano explosivity
Authors: Cassidy Mike , Castro Jonathan M. , Helo Christoph , Troll Valentin R. , Deegan Frances M. , Muir Duncan , Neave David A. , Mueller Sebastian P. 
Published: November 2016

Abstract
Magma reservoirs underneath volcanoes grow through episodic emplacement of magma batches. These pulsed magma injections can substantially alter the physical state of the resident magma by changing its temperature, pressure, composition, and volatile content. Here we examine plagioclase phenocrysts in pumice from the 2014 Plinian eruption of Kelud (Indonesia) that record the progressive capture of small melt inclusions within concentric growth zones during crystallization inside a magma reservoir. High-spatial-resolution Raman spectroscopic measurements reveal the concentration of dissolved H2O within the melt inclusions, and provide insights into melt-volatile behavior at the single crystal scale. H2O contents within melt inclusions range from ∼0.45 to 2.27 wt% and do not correlate with melt inclusion size or distance from the crystal rim, suggesting that minimal H2O was lost via diffusion. Instead, inclusion H2O contents vary systematically with anorthite content of the host plagioclase (R2 = 0.51), whereby high anorthite content zones are associated with low H2O contents and vice versa. This relationship suggests that injections of hot and H2O-poor magma can increase the reservoir temperature, leading to the dilution of melt H2O contents. In addition to recording hot and H2O-poor conditions after these injections, plagioclase crystals also record relatively cold and H2O-rich conditions such as prior to the explosive 2014 eruption. In this case, the elevated H2O content and increased viscosity may have contributed to the high explosivity of the eruption. The point at which an eruption occurs within such repeating hot and cool cycles may therefore have important implications for explaining alternating eruptive styles.
Dysarthria in individuals with Parkinson's disease: a protocol for a binational, cross-sectional, case-controlled study in French and European Portuguese (FraLusoPark)
Authors: Pinto Serge , Cardoso Rita , Sadat Jasmin , Guimarães Isabel , Mercier Céline , Santos Helena , Atkinson-Clement Cyril , Carvalho Joana , Welby Pauline , Oliveira Pedro , D'Imperio Mariapaola , Frota Sónia , Letanneux Alban , Vigario Marina , Cruz Marisa , Martins Isabel Pavão , Viallet François , Ferreira Joaquim J 
Published: November 2016

IntroductionIndividuals with Parkinson's disease (PD) have to deal with several aspects of voice and speech decline and thus alteration of communication ability during the course of the disease. Among these communication impairments, 3 major challenges include: (1) dysarthria, consisting of orofacial motor dysfunction and dysprosody, which is linked to the neurodegenerative processes; (2) effects of the pharmacological treatment, which vary according to the disease stage; and (3) particular speech modifications that may be language-specific, that is, dependent on the language spoken by the patients. The main objective of the FraLusoPark project is to provide a thorough evaluation of changes in PD speech as a result of pharmacological treatment and disease duration in 2 different languages (French vs European Portuguese).Methods and analysisIndividuals with PD are enrolled in the study in France (N=60) and Portugal (N=60). Their global motor disability and orofacial motor functions is assessed with specific clinical rating scales, without (OFF) and with (ON) pharmacological treatment. 2 groups of 60 healthy age-matched volunteers provide the reference for between-group comparisons. Along with the clinical examinations, several speech tasks are recorded to obtain acoustic and perceptual measures. Patient-reported outcome measures are used to assess the psychosocial impact of dysarthria on quality of life.Ethics and disseminationThe study has been approved by the local responsible committees on human experimentation and is conducted in accordance with the ethical standards. A valuable large-scale database of speech recordings and metadata from patients with PD in France and Portugal will be constructed. Results will be disseminated in several articles in peer-reviewed journals and in conference presentations. Recommendations on how to assess speech and voice disorders in individuals with PD to monitor the progression and management of symptoms will be provided.Trial registration numberNCT02753192, Pre-results.