bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hysterectomy and incidence of depressive symptoms in midlife women: the Australian Longitudinal Study on Women's Health
Authors: Wilson L. , Pandeya N. , Byles J. , Mishra G. 
Published: February 2017

Aims.There is limited longitudinal research that has looked at the longer term incidence of depressive symptoms, comparing women with a hysterectomy to women without a hysterectomy. We aimed to investigate the association between hysterectomy status and the 12-year incidence of depressive symptoms in a mid-aged cohort of Australian women, and whether these relationships were modified by use of exogenous hormones.Methods.We used generalised estimating equation models for binary outcome data to assess the associations of the incidence of depressive symptoms (measured by the 10-item Centre for Epidemiologic Studies Depression Scale) across five surveys over a 12-year period, in women with a hysterectomy with ovarian conservation, or a hysterectomy with bilateral oophorectomy compared with women without a hysterectomy. We further stratified women with hysterectomy by their current use of menopausal hormone therapy (MHT). Women who reported prior treatment for depression were excluded from the analysis.Results.Compared with women without a hysterectomy (n = 4002), both women with a hysterectomy with ovarian conservation (n = 884) and women with a hysterectomy and bilateral oophorectomy (n = 450) had a higher risk of depressive symptoms (relative risk (RR) 1.20; 95% confidence interval (CI) 1.06–1.36 and RR 1.44; 95% CI 1.22–1.68, respectively). There were differences in the strength of the risk for women with a hysterectomy with ovarian conservation, compared with those without, when we stratified by current MHT use. Compared with women without a hysterectomy who did not use MHT, women with a hysterectomy with ovarian conservation who were also MHT users had a higher risk of depressive symptoms (RR 1.57; 95% CI 1.31–1.88) than women with a hysterectomy with ovarian conservation but did not use MHT (RR 1.17; 95% CI 1.02–1.35). For women with a hysterectomy and bilateral oophorectomy, MHT use did not attenuate the risk. We could not rule out, however, that the higher risk seen among MHT users may be due to confounding by indication, i.e. MHT was prescribed to treat depressive symptoms, but their depressive symptoms persisted.Conclusions.Women with a hysterectomy (with and without bilateral oophorectomy) have a higher risk of new incidence of depressive symptoms in the longer term that was not explained by lifestyle or socio-economic factors.
A Potential Role of the 5-HTTLPR Polymorphism in Self-Reported Executive Functioning
Authors: Alfimova Margarita V. , Golimbet Vera , Lezheiko Tatyana , Korovaitseva Galina 
Published: February 2017

AbstractIntense effort is directed toward searching for associations between genes and neuropsychological measures of executive functions. In contrast, the impact of genetic polymorphisms on self-rating of everyday executive functioning has not been investigated so far. This study was designed to test associations of self-reported executive functioning, measured with the Behavior Rating Inventory of Executive Function (BRIEF-A), with dopaminergic and serotoninergic genes in non-clinical population and to assess impact of neuropsychological and personality characteristics on these associations. One hundred healthy adults completed the BRIEF-A, personality inventories SPQ-74, STAI, MMPI, and neuropsychological tests for executive functions. Polymorphisms in theDRD4, COMT, DRD2, HTR2A, andSLC6A4genes were genotyped. We revealed a significant main effect of theSLC6A4’s 5-HTTLPR polymorphism on BRIEF-A scores (F= 2.21,P= .018, η2= .24). Among the BRIEF-A measures, the genotype effect was significant for the Plan/Organize (F= 7.34,P= .008, η2= .07) and Task Monitor scales (F= 4.33,P= .04, η2= .04), and the Metacognition index (F= 4.21,P= .043, η2= .04). Carriers of the short allele reported fewer problems than homozygotes for the long allele. Correlations of the BRIEF-A measures with neuropsychological variables were weak, while those with personality characteristics were strong, with trait anxiety being the most powerful predictor of the BRIEF-A scores. However, the relationship between the 5-HTTLPR and BRIEF-A scores remained significant when trait anxiety was controlled for. The results suggest a potential role of the 5-HTTLPR in self-reported everyday task planning and monitoring.
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state
Authors: Rossey Iebe , Gilman Morgan S. A. , Kabeche Stephanie C. , Sedeyn Koen , Wrapp Daniel , Kanekiyo Masaru , Chen Man , Mas Vicente , Spitaels Jan , Melero José A. , Graham Barney S. , Schepens Bert , McLellan Jason S. , Saelens Xavier 
Published: February 2017

AbstractHuman respiratory syncytial virus (RSV) is the main cause of lower respiratory tract infections in young children. The RSV fusion protein (F) is highly conserved and is the only viral membrane protein that is essential for infection. The prefusion conformation of RSV F is considered the most relevant target for antiviral strategies because it is the fusion-competent form of the protein and the primary target of neutralizing activity present in human serum. Here, we describe two llama-derived single-domain antibodies (VHHs) that have potent RSV-neutralizing activity and bind selectively to prefusion RSV F with picomolar affinity. Crystal structures of these VHHs in complex with prefusion F show that they recognize a conserved cavity formed by two F protomers. In addition, the VHHs prevent RSV replication and lung infiltration of inflammatory monocytes and T cells in RSV-challenged mice. These prefusion F-specific VHHs represent promising antiviral agents against RSV.
Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice
Authors: Andres-Hernando Ana , Li Nanxing , Cicerchi Christina , Inaba Shinichiro , Chen Wei , Roncal-Jimenez Carlos , Le Myphuong T. , Wempe Michael F. , Milagres Tamara , Ishimoto Takuji , Fini Mehdi , Nakagawa Takahiko , Johnson Richard J. , Lanaspa Miguel A. 
Published: February 2017

AbstractAcute kidney injury is associated with high mortality, especially in intensive care unit patients. The polyol pathway is a metabolic route able to convert glucose into fructose. Here we show the detrimental role of endogenous fructose production by the polyol pathway and its metabolism through fructokinase in the pathogenesis of ischaemic acute kidney injury (iAKI). Consistent with elevated urinary fructose in AKI patients, mice undergoing iAKI show significant polyol pathway activation in the kidney cortex characterized by high levels of aldose reductase, sorbitol and endogenous fructose. Wild type but not fructokinase knockout animals demonstrate severe kidney injury associated with ATP depletion, elevated uric acid, oxidative stress and inflammation. Interestingly, both the renal injury and dysfunction in wild-type mice undergoing iAKI is significantly ameliorated when exposed to luteolin, a recently discovered fructokinase inhibitor. This study demonstrates a role for fructokinase and endogenous fructose as mediators of acute renal disease.
A metamorphic inorganic framework that can be switched between eight single-crystalline states
Authors: Zhan Caihong , Cameron Jamie M. , Gabb David , Boyd Thomas , Winter Ross S. , Vilà-Nadal Laia , Mitchell Scott G. , Glatzel Stefan , Breternitz Joachim , Gregory Duncan H. , Long De-Liang , Macdonell Andrew , Cronin Leroy 
Published: February 2017

AbstractThe design of highly flexible framework materials requires organic linkers, whereas inorganic materials are more robust but inflexible. Here, by using linkable inorganic rings made up of tungsten oxide (P8W48O184) building blocks, we synthesized an inorganic single crystal material that can undergo at least eight different crystal-to-crystal transformations, with gigantic crystal volume contraction and expansion changes ranging from −2,170 to +1,720 Å3 with no reduction in crystallinity. Not only does this material undergo the largest single crystal-to-single crystal volume transformation thus far reported (to the best of our knowledge), the system also shows conformational flexibility while maintaining robustness over several cycles in the reversible uptake and release of guest molecules switching the crystal between different metamorphic states. This material combines the robustness of inorganic materials with the flexibility of organic frameworks, thereby challenging the notion that flexible materials with robustness are mutually exclusive.
Modulating supramolecular binding of carbon dioxide in a redox-active porous metal-organic framework
Authors: Lu Zhenzhong , Godfrey Harry G. W. , da Silva Ivan , Cheng Yongqiang , Savage Mathew , Tuna Floriana , McInnes Eric J. L. , Teat Simon J. , Gagnon Kevin J. , Frogley Mark D. , Manuel Pascal , Rudić Svemir , Ramirez-Cuesta Anibal J. , Easun Timothy L. , Yang Sihai , Schröder Martin 
Published: February 2017

AbstractHydrogen bonds dominate many chemical and biological processes, and chemical modification enables control and modulation of host–guest systems. Here we report a targeted modification of hydrogen bonding and its effect on guest binding in redox-active materials. MFM-300(VIII) {[VIII2(OH)2(L)], LH4=biphenyl-3,3′,5,5′-tetracarboxylic acid} can be oxidized to isostructural MFM-300(VIV), [VIV2O2(L)], in which deprotonation of the bridging hydroxyl groups occurs. MFM-300(VIII) shows the second highest CO2 uptake capacity in metal-organic framework materials at 298 K and 1 bar (6.0 mmol g−1) and involves hydrogen bonding between the OH group of the host and the O-donor of CO2, which binds in an end-on manner, "Equation missing"=1.863(1) Å. In contrast, CO2-loaded MFM-300(VIV) shows CO2 bound side-on to the oxy group and sandwiched between two phenyl groups involving a unique "Equation missing"···c.g.phenyl interaction [3.069(2), 3.146(3) Å]. The macroscopic packing of CO2 in the pores is directly influenced by these primary binding sites.
Advanced rechargeable aluminium ion battery with a high-quality natural graphite cathode
Authors: Wang Di-Yan , Wei Chuan-Yu , Lin Meng-Chang , Pan Chun-Jern , Chou Hung-Lung , Chen Hsin-An , Gong Ming , Wu Yingpeng , Yuan Chunze , Angell Michael , Hsieh Yu-Ju , Chen Yu-Hsun , Wen Cheng-Yen , Chen Chun-Wei , Hwang Bing-Joe , Chen Chia-Chun , Dai Hongjie 
Published: February 2017

AbstractRecently, interest in aluminium ion batteries with aluminium anodes, graphite cathodes and ionic liquid electrolytes has increased; however, much remains to be done to increase the cathode capacity and to understand details of the anion–graphite intercalation mechanism. Here, an aluminium ion battery cell made using pristine natural graphite flakes achieves a specific capacity of ∼110 mAh g−1 with Coulombic efficiency ∼98%, at a current density of 99 mA g−1 (0.9 C) with clear discharge voltage plateaus (2.25–2.0 V and 1.9–1.5 V). The cell has a capacity of 60 mAh g−1 at 6 C, over 6,000 cycles with Coulombic efficiency ∼ 99%. Raman spectroscopy shows two different intercalation processes involving chloroaluminate anions at the two discharging plateaus, while C–Cl bonding on the surface, or edges of natural graphite, is found using X-ray absorption spectroscopy. Finally, theoretical calculations are employed to investigate the intercalation behaviour of choloraluminate anions in the graphite electrode.
Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity
Authors: Zamarin Dmitriy , Holmgaard Rikke B. , Ricca Jacob , Plitt Tamar , Palese Peter , Sharma Padmanee , Merghoub Taha , Wolchok Jedd D. , Allison James P. 
Published: February 2017

AbstractEmerging data suggest that locoregional cancer therapeutic approaches with oncolytic viruses can lead to systemic anti-tumour immunity, although the appropriate targets for intratumoral immunomodulation using this strategy are not known. Here we find that intratumoral therapy with Newcastle disease virus (NDV), in addition to the activation of innate immunity, upregulates the expression of T-cell co-stimulatory receptors, with the inducible co-stimulator (ICOS) being most notable. To explore ICOS as a direct target in the tumour, we engineered a recombinant NDV-expressing ICOS ligand (NDV-ICOSL). In the bilateral flank tumour models, intratumoral administration of NDV-ICOSL results in enhanced infiltration with activated T cells in both virus-injected and distant tumours, and leads to effective rejection of both tumours when used in combination with systemic CTLA-4 blockade. These findings highlight that intratumoral immunomodulation with an oncolytic virus expressing a rationally selected ligand can be an effective strategy to drive systemic efficacy of immune checkpoint blockade.
Self-organizing actin patterns shape membrane architecture but not cell mechanics
Authors: Fritzsche M. , Li D. , Colin-York H. , Chang V. T. , Moeendarbary E. , Felce J. H. , Sezgin E. , Charras G. , Betzig E. , Eggeling C. 
Published: February 2017

AbstractCell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeLa cells. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. This process is primarily driven by Arp2/3 complex nucleation, but not by myosin motors, which is in contrast to what has been theoretically predicted and observed in vitro. Concomitant measurements of mechanics and plasma membrane fluidity demonstrate that changes in actin patterning alter membrane architecture but occur functionally independent of macroscopic cortex elasticity. Consequently, tuning the activity of the Arp2/3 complex to alter filament assembly may thus be a mechanism allowing cells to adjust their membrane architecture without affecting their macroscopic mechanical properties.
Recurrently deregulated lncRNAs in hepatocellular carcinoma
Authors: Yang Yang , Chen Lei , Gu Jin , Zhang Hanshuo , Yuan Jiapei , Lian Qiuyu , Lv Guishuai , Wang Siqi , Wu Yang , Yang Yu-Cheng T. , Wang Dongfang , Liu Yang , Tang Jing , Luo Guijuan , Li Yang , Hu Long , Sun Xinbao , Wang Dong , Guo Mingzhou , Xi Qiaoran , Xi Jianzhong , Wang Hongyang , Zhang Michael Q. , Lu Zhi John 
Published: February 2017

AbstractHepatocellular carcinoma (HCC) cells often invade the portal venous system and subsequently develop into portal vein tumour thrombosis (PVTT). Long noncoding RNAs (lncRNAs) have been associated with HCC, but a comprehensive analysis of their specific association with HCC metastasis has not been conducted. Here, by analysing 60 clinical samples’ RNA-seq data from 20 HCC patients, we have identified and characterized 8,603 candidate lncRNAs. The expression patterns of 917 recurrently deregulated lncRNAs are correlated with clinical data in a TCGA cohort and published liver cancer data. Matched array data from the 60 samples show that copy number variations (CNVs) and alterations in DNA methylation contribute to the observed recurrent deregulation of 235 lncRNAs. Many recurrently deregulated lncRNAs are enriched in co-expressed clusters of genes related to cell adhesion, immune response and metabolic processes. Candidate lncRNAs related to metastasis, such as HAND2-AS1, were further validated using RNAi-based loss-of-function assays. Thus, we provide a valuable resource of functional lncRNAs and biomarkers associated with HCC tumorigenesis and metastasis.
CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor
Authors: Funnell Tyler , Tasaki Shinya , Oloumi Arusha , Araki Shinsuke , Kong Esther , Yap Damian , Nakayama Yusuke , Hughes Christopher S. , Cheng S.-W. Grace , Tozaki Hirokazu , Iwatani Misa , Sasaki Satoshi , Ohashi Tomohiro , Miyazaki Tohru , Morishita Nao , Morishita Daisuke , Ogasawara-Shimizu Mari , Ohori Momoko , Nakao Shoichi , Karashima Masatoshi , Sano Masaya , Murai Aiko , Nomura Toshiyuki , Uchiyama Noriko , Kawamoto Tomohiro , Hara Ryujiro , Nakanishi Osamu , Shumansky Karey , Rosner Jamie , Wan Adrian , McKinney Steven , Morin Gregg B. , Nakanishi Atsushi , Shah Sohrab , Toyoshiba Hiroyoshi , Aparicio Samuel 
Published: February 2017

AbstractCDC-like kinase phosphorylation of serine/arginine-rich proteins is central to RNA splicing reactions. Yet, the genomic network of CDC-like kinase-dependent RNA processing events remains poorly defined. Here, we explore the connectivity of genomic CDC-like kinase splicing functions by applying graduated, short-exposure, pharmacological CDC-like kinase inhibition using a novel small molecule (T3) with very high potency, selectivity, and cell-based stability. Using RNA-Seq, we define CDC-like kinase-responsive alternative splicing events, the large majority of which monotonically increase or decrease with increasing CDC-like kinase inhibition. We show that distinct RNA-binding motifs are associated with T3 response in skipped exons. Unexpectedly, we observe dose-dependent conjoined gene transcription, which is associated with motif enrichment in the last and second exons of upstream and downstream partners, respectively. siRNA knockdown of CLK2-associated genes significantly increases conjoined gene formation. Collectively, our results reveal an unexpected role for CDC-like kinase in conjoined gene formation, via regulation of 3′-end processing and associated splicing factors.
Longitudinal test-retest neuroimaging data from healthy young adults in southwest China
Authors: Liu Wei , Wei Dongtao , Chen Qunlin , Yang Wenjing , Meng Jie , Wu Guorong , Bi Taiyong , Zhang Qinglin , Zuo Xi-Nian , Qiu Jiang 
Published: February 2017

AbstractMultimodal magnetic resonance imaging (mMRI) has been widely used to map the structure and function of the human brain, as well as its behavioral associations. However, to date, a large sample with a long-term longitudinal design and a narrow age-span has been lacking for the assessment of test-retest reliability and reproducibility of brain-behavior correlations, as well as the development of novel causal insights into these correlational findings. Here we describe the SLIM dataset, which includes brain and behavioral data across a long-term retest-duration within three and a half years, mMRI scans provided a set of structural, diffusion and resting-state functional MRI images, along with rich samples of behavioral assessments addressed—demographic, cognitive and emotional information. Together with the Consortium for Reliability and Reproducibility (CoRR), the SLIM is expected to accelerate the reproducible sciences of the human brain by providing an open resource for brain-behavior discovery sciences with big-data approaches.
Inhibitory effects of cholinesterase inhibitor donepezil on the Kv1.5 potassium channel
Authors: Li Kai , Cheng Neng , Li Xian-Tao 
Published: February 2017

AbstractKv1.5 channels carry ultra-rapid delayed rectifier K+ currents in excitable cells, including neurons and cardiac myocytes. In the current study, the effects of cholinesterase inhibitor donepezil on cloned Kv1.5 channels expressed in HEK29 cells were explored using whole-cell recording technique. Exposure to donepezil resulted in a rapid and reversible block of Kv1.5 currents, with an IC50 value of 72.5 μM. The mutant R476V significantly reduced the binding affinity of donepezil to Kv1.5 channels, showing the target site in the outer mouth region. Donepezil produced a significant delay in the duration of activation and deactivation, and mutant R476V potentiated these effects without altering activation curves. In response to slowed deactivation time course, a typical crossover of Kv1.5 tail currents was clearly evident after bath application of donepezil. In addition, both this chemical and mutant R476V accelerated current decay during channel inactivation in a voltage-dependent way, but barely changed the inactivation and recovery curves. The presence of donepezil exhibited the use-dependent block of Kv1.5 currents in response to a series of depolarizing pulses. Our data indicate that donepezil can directly block Kv1.5 channels in its open and closed states.
Arrays of Individual DNA Molecules on Nanopatterned Substrates
Authors: Hager Roland , Halilovic Alma , Burns Jonathan R. , Schäffler Friedrich , Howorka Stefan 
Published: February 2017

AbstractArrays of individual molecules can combine the advantages of microarrays and single-molecule studies. They miniaturize assays to reduce sample and reagent consumption and increase throughput, and additionally uncover static and dynamic heterogeneity usually masked in molecular ensembles. However, realizing single-DNA arrays must tackle the challenge of capturing structurally highly dynamic strands onto defined substrate positions. Here, we create single-molecule arrays by electrostatically adhering single-stranded DNA of gene-like length onto positively charged carbon nanoislands. The nanosites are so small that only one molecule can bind per island. Undesired adsorption of DNA to the surrounding non-target areas is prevented via a surface-passivating film. Of further relevance, the DNA arrays are of tunable dimensions, and fabricated on optically transparent substrates that enable singe-molecule detection with fluorescence microscopy. The arrays are hence compatible with a wide range of bioanalytical, biophysical, and cell biological studies where individual DNA strands are either examined in isolation, or interact with other molecules or cells.
Investigating carrier localization and transfer in InGaN/GaN quantum wells with V-pits using near-field scanning optical microscopy and correlation analysis
Authors: Kim MinKwan , Choi Sunghan , Lee Joo-Hyung , Park ChungHyun , Chung Tae-Hoon , Baek Jong Hyeob , Cho Yong-Hoon 
Published: February 2017

AbstractThe V-pits and potential fluctuations in InGaN/GaN multiple quantum wells (MQWs) are key factors for understanding the performance of InGaN/GaN-based light-emitting diodes (LEDs). However, photoluminescence (PL) measurements using conventional optical microscopy only provide ensemble information due to the spatial resolution limit, known as the diffraction barrier, which hinders the analysis of dislocations and potential fluctuations. Here, in order to investigate the influence of the V-pits and potential fluctuations on local optical properties, we performed nanoscopic luminescence mapping for standard and V-pit InGaN/GaN MQWs samples with different sized V-pits using near-field scanning optical microscopy (NSOM) with illumination mode (I-mode) at various laser excitation powers. From the nanoscopic PL mapping data, we could clearly observe luminescence features associated with dislocations and potential fluctuations in the InGaN/GaN MQWs. We also employed correlation analysis to quantitatively analyze the nanoscopic PL mapping data for the different MQWs samples. Based on the results of NSOM PL with I-mode and correlation analysis, we could demonstrate that carrier transfer in the MQWs sample with large sized V-pits is suppressed by deeper potential fluctuations and higher energy barriers compared to the standard sample.