bluuebunny/crossref_metadata_2025_split huggingface dataset:
Afferent motor feedback determines the perceived location of tactile stimuli in the external space presented to the moving arm
Authors: Maij Femke , Wing Alan M. , Medendorp W. Pieter 
Published: March 2017

People make systematic errors when localizing a brief tactile stimulus in the external space presented on the index finger while moving the arm. Although these errors likely arise in the spatiotemporal integration of the tactile input and information about arm position, the underlying arm position information used in this process is not known. In this study, we tested the contributions of afferent proprioceptive feedback and predictive arm position signals by comparing localization errors during passive vs. active arm movements. In the active trials, participants were instructed to localize a tactile stimulus in the external space that was presented to the index finger near the time of a self-generated arm movement. In the passive trials, each of the active trials was passively replayed in randomized order, using a robotic device. Our results provide evidence that the localization error patterns of the passive trials are similar to the active trials and, moreover, did not lag but rather led the active trials, which suggests that proprioceptive feedback makes an important contribution to tactile localization. To further test which kinematic property of this afferent feedback signal drives the underlying computations, we examined the localization errors with movements that had differently skewed velocity profiles but overall the same displacement. This revealed a difference in the localization patterns, which we explain by a probabilistic model in which temporal uncertainty about the stimulus is converted into a spatial likelihood, depending on the actual velocity of the arm rather than involving an efferent, preprogrammed movement. NEW & NOTEWORTHY We show that proprioceptive feedback of arm motion rather than efferent motor signals contributes to tactile localization during an arm movement. Data further show that localization errors depend on arm velocity, not displacement per se, suggesting that instantaneous velocity feedback plays a role in the underlying computations. Model simulation using Bayesian inference suggests that these errors depend not only on spatial but also on temporal uncertainties of sensory and motor signals.
Long Noncoding RNA MANTIS Facilitates Endothelial Angiogenic Function
Authors: Leisegang Matthias S. , Fork Christian , Josipovic Ivana , Richter Florian Martin , Preussner Jens , Hu Jiong , Miller Matthew J. , Epah Jeremy , Hofmann Patrick , Günther Stefan , Moll Franziska , Valasarajan Chanil , Heidler Juliana , Ponomareva Yuliya , Freiman Thomas M. , Maegdefessel Lars , Plate Karl H. , Mittelbronn Michel , Uchida Shizuka , Künne Carsten , Stellos Konstantinos , Schermuly Ralph T. , Weissmann Norbert , Devraj Kavi , Wittig Ilka , Boon Reinier A. , Dimmeler Stefanie , Pullamsetti Soni Savai , Looso Mario , Miller Francis J. , Brandes Ralf P. 
Published: March 2017

Background:
The angiogenic function of endothelial cells is regulated by numerous mechanisms, but the impact of long noncoding RNAs (lncRNAs) has hardly been studied. We set out to identify novel and functionally important endothelial lncRNAs.

Methods:
Epigenetically controlled lncRNAs in human umbilical vein endothelial cells were searched by exon-array analysis after knockdown of the histone demethylase JARID1B. Molecular mechanisms were investigated by RNA pulldown and immunoprecipitation, mass spectrometry, microarray, several knockdown approaches, CRISPR-Cas9, assay for transposase-accessible chromatin sequencing, and chromatin immunoprecipitation in human umbilical vein endothelial cells. Patient samples from lung and tumors were studied for MANTIS expression.

Results:

A search for epigenetically controlled endothelial lncRNAs yielded lncRNA n342419, here termed MANTIS, as the most strongly regulated lncRNA. Controlled by the histone demethylase JARID1B, MANTIS was downregulated in patients with idiopathic pulmonary arterial hypertension and in rats treated with monocrotaline, whereas it was upregulated in carotid arteries of
Macaca fascicularis
subjected to atherosclerosis regression diet, and in endothelial cells isolated from human glioblastoma patients. CRISPR/Cas9-mediated deletion or silencing of MANTIS with small interfering RNAs or GapmeRs inhibited angiogenic sprouting and alignment of endothelial cells in response to shear stress. Mechanistically, the nuclear-localized MANTIS lncRNA interacted with BRG1, the catalytic subunit of the switch/sucrose nonfermentable chromatin-remodeling complex. This interaction was required for nucleosome remodeling by keeping the ATPase function of BRG1 active. Thereby, the transcription of key endothelial genes such as
SOX18
,
SMAD6
, and
COUP-TFII
was regulated by ensuring efficient RNA polymerase II machinery binding.

Conclusion:
MANTIS is a differentially regulated novel lncRNA facilitating endothelial angiogenic function.
Internal-Wave-Driven Mixing: Global Geography and Budgets
Authors: Kunze Eric 
Published: March 2017

AbstractInternal-wave-driven dissipation rates ε and diapycnal diffusivities K are inferred globally using a finescale parameterization based on vertical strain applied to ~30 000 hydrographic casts. Global dissipations are 2.0 ± 0.6 TW, consistent with internal wave power sources of 2.1 ± 0.7 TW from tides and wind. Vertically integrated dissipation rates vary by three to four orders of magnitude with elevated values over abrupt topography in the western Indian and Pacific as well as midocean slow spreading ridges, consistent with internal tide sources. But dependence on bottom forcing is much weaker than linear wave generation theory, pointing to horizontal dispersion by internal waves and relatively little local dissipation when forcing is strong. Stratified turbulent bottom boundary layer thickness variability is not consistent with OGCM parameterizations of tidal mixing. Average diffusivities K = (0.3–0.4) × 10−4 m2 s−1 depend only weakly on depth, indicating that ε = KN2/γ scales as N2 such that the bulk of the dissipation is in the pycnocline and less than 0.08-TW dissipation below 2000-m depth. Average diffusivities K approach 10−4 m2 s−1 in the bottom 500 meters above bottom (mab) in height above bottom coordinates with a 2000-m e-folding scale. Average dissipation rates ε are 10−9 W kg−1 within 500 mab then diminish to background deep values of 0.15 × 10−9 W kg−1 by 1000 mab. No incontrovertible support is found for high dissipation rates in Antarctic Circumpolar Currents or parametric subharmonic instability being a significant pathway to elevated dissipation rates for semidiurnal or diurnal internal tides equatorward of 28° and 14° latitudes, respectively, although elevated K is found about 30° latitude in the North and South Pacific.
Observation System Experiments with the Hourly Updating Rapid Refresh Model Using GSI Hybrid Ensemble–Variational Data Assimilation
Authors: James Eric P. , Benjamin Stanley G. 
Published: March 2017

Abstract
A set of observation system experiments (OSEs) over three seasons using the hourly updated Rapid Refresh (RAP) numerical weather prediction (NWP) assimilation–forecast system identifies the importance of the various components of the North American observing system for 3–12-h RAP forecasts. Aircraft observations emerge as the strongest-impact observation type for wind, relative humidity (RH), and temperature forecasts, permitting a 15%–30% reduction in 6-h forecast error in the troposphere and lower stratosphere. Major positive impacts are also seen from rawinsondes, GOES satellite cloud observations, and surface observations, with lesser but still significant impacts from GPS precipitable water (PW) observations, satellite atmospheric motion vectors (AMVs), and radar reflectivity observations. A separate experiment revealed that the aircraft-related RH forecast improvement was augmented by 50% due specifically to the addition of aircraft moisture observations. Additionally, observations from en route aircraft and those from ascending or descending aircraft contribute approximately equally to the overall forecast skill, with the strongest impacts in the respective layers of the observations. Initial results from these OSEs supported implementation of an improved assimilation configuration of boundary layer pseudoinnovations from surface observations, as well as allowing the assimilation of satellite AMVs over land. The breadth of these experiments over the three seasons suggests that observation impact results are applicable to general forecasting skill, not just classes of phenomena during limited time periods.
Youth self-harm in low- and middle-income countries: Systematic review of the risk and protective factors
Authors: Aggarwal Shilpa , Patton George , Reavley Nicola , Sreenivasan Shreenivas A , Berk Michael 
Published: March 2017

Background: Self-harm (defined here as an act of intentionally causing harm to own self, irrespective of the type, motive or suicidal intent) is one of the strongest antecedents of suicide in youth. While there have been a number of studies of youth self-harm in low- and middle-income countries (LMICs), there is currently no systematic review of studies of prevalence rates and risk and protective factors. Aim: To systematically review the evidence relating to the prevalence rates and forms of self-harm in youth in LMICs and its relationship to family economic status, family functioning, relationship with the peer group, social relationships and academic performance. Methods: Electronic searches of three databases, MEDLINE, PsycINFO and Scopus, were performed. In total, 27 school-, community- and hospital-based studies evaluating self-harm in LMICs with youth focus (with participants between 12 and 25 years) were included. Results: The self-harm was divided into suicidal and non-suicidal depending on the nature of self-harm. The 12-month prevalence rates of non-suicidal self-harm varied from 15.5% to 31.3%, and the range of suicidal behaviour rates was from 3.2% to 4.7%. Banging and hitting were the most common in the community-based studies, followed by cutting, scratching and wound picking. Self-poisoning with pesticides was most commonly reported in hospital-based studies. Risk factors for self-harm were family conflict, peer groups with members indulging in self-harm, truancy and school absenteeism. Protective factors were having an understanding family, having friends and higher school competence. Risk factors for suicidal thoughts and attempts were lack of close friends and history of suicide by a friend. Conclusion: The 12-month prevalence rates of youth self-harm in LMICs are comparable to high-income countries (HICs). The profile of risk and protective factors suggests that family-based interventions could be useful in treatment and prevention. Future studies should aim for greater consistency in assessment methods and the constructs under evaluation.
The synergy of the refugee crisis and the financial crisis in Greece: Impact on mental health
Authors: Anagnostopoulos Dimitris C , Giannakopoulos George , Christodoulou Nikos G 
Published: March 2017

Background: The current global financial crisis that started in 2008 resulted in a significant decline in global trade, slowing/reversing economic growth worldwide, and a dramatic increase in public sector debt. At the same time, the global migrant/refugee crisis has reached extreme rates, with millions of people being forced to abandon their homes and communities because of war, political violence or related threats. There is a broad consensus about the deleterious consequences of these crises on psychological well-being, depression, anxiety disorders, insomnia, alcohol abuse and suicidal behavior. Although the separate consequences of economic recession and immigration are extensively discussed in previous research, we know very little about the processes through which the intersection of economic crisis and migrant crisis contributes to the vulnerabilities of natives and migrants during these crises. Of particular concern is the status of children, adolescents and their families, who constitute one of the most vulnerable groups in society. Aim: To discuss the contexts that economic and migrant crises shape and suggest possible effects of this intersection on mental health risks, especially among children, adolescents and their families, through reflecting on the recent experience in Greece. Method: Review of the literature and critical analysis of the effects of the confluent crises. Conclusion: The interactive effects of these two crises need further exploration. Novel and diverse models of psychological understanding need to be developed in order to manage the effects of the confluent crises. The role of mental health professionals is crucial in this respect, offering culturally flexible, accommodating and empathetic approaches, allowing healing and acceptance in the face of adversity.
Heparanase overexpression down-regulates syndecan-1 expression in a gallbladder carcinoma cell line
Authors: Jin Hao , Zhou Shaobo , Yang Song , Cao Hai-ming 
Published: March 2017

Objective To discuss the relevance of heparanase and syndecan-1 and regulation of the heparanase-syndecan1 axis in the invasiveness of gallbladder carcinoma cells. Methods 1. Generation of a gallbladder cancer cell line overexpressing a heparanase (GBD-SD) transgene. 2. Western blot analysis of syndecan-1 levels of GBD-SD and control gallbladder carcinoma (GBC-SD) cells. 3. RT-PCR analysis of syndecan-1 mRNA levels of GBD-SD and GBC-SD. 4. Evaluation of invasion and migration of GBD-SD and GBC-SD cells. Results 1. Heparanase expression in GBD-SD cells was significantly increased. 2. The syndecan-1 mRNA level of GBD-SD cells was significantly lower compared with that of GBC-SD cells. 3. The syndecan-1 DNA copy number in GBD-SD cells was significantly lower compared with that of GBC-SD. 4. The invasiveness and migration of GBD-SD cells were significantly higher compared with GBC-SD cells. Conclusions 1. The expression of heparanase negatively correlated with that of syndecan-1 in a gallbladder carcinoma cell line. 2. The expression of heparanase and syndecan-1 in gallbladder carcinomas negatively correlated, similar to other tumours. 3. The heparanase/syndecan1 axis in gallbladder carcinoma plays an important role in the invasion and metastasis, thus providing a new therapeutic target. 4. Further research is required to identify the detailed mechanisms.
Presence of Bacteria in Spontaneous Achilles Tendon Ruptures
Authors: Rolf Christer G. , Fu Sai-Chuen , Hopkins Chelsea , Luan Ju , Ip Margaret , Yung Shu-Hang , Friman Göran , Qin Ling , Chan Kai-Ming 
Published: March 2017

Background: The structural pathology of Achilles tendon (AT) ruptures resembles tendinopathy, but the causes remain unknown. Recently, a number of diseases were found to be attributed to bacterial infections, resulting in low-grade inflammation and progressive matrix disturbance. The authors speculate that spontaneous AT ruptures may also be influenced by the presence of bacteria. Hypothesis: Bacteria are present in ruptured ATs but not in healthy tendons. Study Design: Cross-sectional study; Level of evidence, 3. Methods: Patients with spontaneous AT ruptures and patients undergoing anterior cruciate ligament (ACL) reconstruction were recruited for this study. During AT surgical repair, excised tendinopathic tissue was collected, and healthy tendon samples were obtained as controls from hamstring tendon grafts used in ACL reconstruction. Half of every sample was reserved for DNA extraction and the other half for histology. Polymerase chain reaction (PCR) was conducted using 16S rRNA gene universal primers, and the PCR products were sequenced for the identification of bacterial species. A histological examination was performed to compare tendinopathic changes in the case and control samples. Results: Five of 20 AT rupture samples were positive for the presence of bacterial DNA, while none of the 23 hamstring tendon samples were positive. Sterile operating and experimental conditions and tests on samples, controlling for harvesting and processing procedures, ruled out the chance of postoperative bacterial contamination. The species identified predominantly belonged to the Staphylococcus genus. AT rupture samples exhibited histopathological features characteristic of tendinopathy, and most healthy hamstring tendon samples displayed normal tendon features. There were no apparent differences in histopathology between the bacterial DNA–positive and bacterial DNA–negative AT rupture samples. Conclusion: The authors have demonstrated the presence of bacterial DNA in ruptured AT samples. It may suggest the potential involvement of bacteria in spontaneous AT ruptures.
Weathering evolution in lutites of the K/Pg transition red beds of the Tremp Group (Tremp-Isona Basin, south Pyrenees)
Authors: Bastida J. , Linares R. , López Buendía A.M. , Osácar M.C. , Rosell J. , Zarroca M. 
Published: March 2017

AbstractThe Tremp–Isona basin (south-central Pyrenees, Lleida, Spain) shows maximum development of the Tremp Group (early Maastrichtian to late Paleocene) covering a wide geological record across the Cretaceous–Paleogene (K/Pg) boundary in continental facies. The mineralogy and geochemistry of lutites were used to assess the evolution of weathering from the Maastrichtian to the Eocene, and particularly for the red beds of the Lower Red and Upper Red Units (pre- and post-K/Pg, respectively). Chemical weathering decreased initially in the Maastrichtian (Gray Unit to Lower Red Unit), increasing subsequently from the Paleocene (Upper Red Unit) to Eocene units. ANOVA analysis of mineralogical compositions and cluster hierarchical analysis have been useful tools to select convenient lutites for assessment of weathering evolution by the chemical alteration index and by broadening of the 001 X-ray diffraction line of illite after ethylene glycol solvation. The lesser chemical weathering found in the Upper Red Unit (Danian) is interpreted in the context of relative warming and aridification, in climates of contrasting seasons and pronounced dry seasons.
Tracking intruder ship in wireless environment
Authors: Rao Madhuri , Kamila Narendra Kumar 
Published: March 2017

AbstractIt is a challenging task for all Harbors or Naval Administration to restrict and monitor the movement of defense or commercial ships. Most commonly used techniques of monitoring are radars and satellite images. These techniques are not reliable as radars can be turned off voluntarily and receptions of images are affected by adverse climatic conditions. This paper proposes a reliable ship intruder detection algorithm that classifies different types of objects approaching the model system in and out of phase with the ocean waves. The proposed technique also takes care of superimposition of temporal and spatial values of nodes that are presumably deployed in the sea surface up to a certain distance. Simulation results prove that the proposed algorithm detects and classifies objects efficiently even when 50% of the nodes reporting the tracking phenomenon are tampered.
Can floseal™ be applied safely during otologic surgery? Assessment of ototoxicity in a chinchilla animal model
Authors: Nhan Carol , Bezdjian Aren , Alarfaj Abdullah , Daniel Sam J. 
Published: March 2017

Background In otologic surgery good visualization is paramount, and patients with bleeding diatheses or who need to be anti-coagulated can present a significant challenge. Here, we determine whether Floseal™, a hemostatic matrix, is ototoxic in a validated animal model. Methods Nine chinchillas housed in the animal care facilities of the Montreal Children's Hospital Research Institute were used for the study. After a myringotomy incision was made in each tympanic membrane, baseline auditory brainstem response measurements were performed at 8, 20, and 25 kHz. In each animal one ear was randomized to receive Floseal™ to the middle ear cavity, whereas the other ear served as the control and received 0.9% sodium chloride. Outcome measures included early (day 7) and late (day 30) auditory brainstem response, clinical evidence of facial nerve or vestibular disturbance and histological evidence of ototoxity. Results There was no significant hearing threshold shift on auditory brainstem response across all tested frequencies for both experimental and control ear. No animals receiving Floseal™ developed facial or vestibular nerve dysfunction and there was no histological evidence of ototoxicity. Conclusion Based on the preliminary ototoxicity assessment on nine chinchillas, transtympanic Floseal™ does not appear to be ototoxic. More studies are warranted to assess the safety and applicability of the product in humans.
Prostate cancer meta-analysis from more than 145,000 men to identify 65 novel prostate cancer susceptibility loci.
Authors: Eeles Rosalind , Al Olama Ali Amin , Berndt Sonja , Wiklund Fredrik , Conti David V , Ahmed Mahbubl , Benlloch Sara , Easton Douglas , Kraft Peter , Chanock Stephen J. , Kote-Jarai Zsofia , Haiman Christopher , Schumacher Fredrick R 
Published: March 2017

1 Background: Currently genome-wide association studies (GWAS) have identified over 100 prostate cancer (PrCa) susceptibility loci, capturing 33% of the PrCa familial relative risk (FRR) in Europeans. To identify further susceptibility variants, we conducted a PrCa GWAS, larger than previous studies, comprising ~49,000 cases and ~29,000 controls among individuals of European and Asian descent using the OncoArray, a platform consisting of a 260K GWAS backbone and 310K custom content selected from previous GWAS and fine-mapping studies of multiple cancers ( http://epi.grants.cancer.gov/oncoarray/ ). Methods: Genotypes from the OncoArray were used to impute genotypes from ~70M variants using the October 2014 release of the 1000 genomes project as a reference, and then combined with several previous PrCa GWAS of European ancestry: UK stage 1 (1,906 cases/1,934 controls) and stage 2 (3,888 cases/3,956 controls); CaPS 1 (498 cases/502 controls) and CaPS 2 (1,483 cases/519 controls); BPC3 (2,137 cases/3,101 controls); NCI PEGASUS (4,622 cases/2,954 controls); and iCOGS (21,209 cases/ 20,440 controls). Risk analyses for overall PrCa risk, aggressive PrCa (several definitions defined by PrCa clinical characteristics), and Gleason score were performed. Logistic and linear regression summary statistics were meta-analysed using an inverse variance fixed effect approach. Results: We identified novel loci significantly associated ( P < 5.0x10-8) with overall PrCa (N = 65). Our novel findings are comprised of several missense variants, including a SNP in the ATM gene - a key member of the DNA repair pathway. When combined multiplicatively, the 65 novel PrCa loci identified here increases the captured heritability of PrCa, explaining 38.5% of the FRR when combining novel and previously identified PrCa loci. Conclusions: In risk stratification, men in the top 1% of the genetic risk score group have a relative risk of 5.6 fold for developing PrCa compared with the median risk group. These results will improve the utility of genetic risk scores for targeted screening and prevention for prostate cancer.