bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revisión del tema de sinergias corporativas: origen, resultados y beneficiarios
Authors: Rivas Luz Maria , Londoño Correa Diana 
Published: June 2017

La materialización de sinergias es un reto para la administración de empresas multinegocios. En este texto se presenta el estado del conocimiento sobre las sinergias en el subcampo de la estrategia corporativa. Para ello se estudian artículos publicados entre 2003 y 2015 en revistas académicas, reconocidas por el Institute for Scientific Información ISI Web of Science y por Scimago. Esta revisión de tema diferencia las investigaciones que abordan las sinergias como resultado en la etapa de adquisición y se centra en la de integración, donde la práctica administrativa toma mayor relevancia. Por lo tanto, se propone estudiarlas sinergias a partir de criterios de clasificación identificados en la literatura: origen, resultados y beneficiarios. De esta forma, reconocer la diversidad de sinergias abre un camino para enfrentar el reto de la administración multinegocios.
No Difference Between Noxious and Innocuous Thermal Stimulation on Motor Recovery of Upper Extremity in Patients With Acute Stroke: A Randomized Controlled Trial With 6‐Month Follow‐up
Authors: Lin Roxane , Hsu Miao‐Ju , Lin Ruey‐Tay , Huang Mao‐Hsiung , Koh Chia‐Lin , Hsieh Ching‐Lin , Lin Jau‐Hong 
Published: June 2017

AbstractBackgroundThermal stimulation (TS) has been developed and incorporated into stroke rehabilitation. However, whether noxious and innocuous TS induce the same effects on motor function recovery after stroke is still unknown. A comparative study of different temperature combination regimens is needed.ObjectiveTo compare the short‐ and long‐term effectiveness between noxious and innocuous TS on motor recovery of upper extremity in patients with acute stroke.DesignRandomized, controlled trial with concealed allocation, intention‐to‐treat analysis and blinded outcome assessors.SettingA university hospital rehabilitation department in Taiwan.ParticipantsA total of 79 patients with acute ischemic stroke were recruited. The majority had moderate to severe motor impairment of the upper extremity (UE).InterventionIn addition to traditional rehabilitation, the experimental group (n = 39) underwent noxious TS (heat pain 46‐47°C/cold pain 7‐8°C), and the control group (n = 40) received innocuous TS (heat 40‐41°C/cold 20‐21°C). TS intervention was applied for 30 minutes once per day and for a total of 20‐24 times during hospital stay. A custom‐made TS instrument, comprising 2 thermal stimulators and their respective thermal pads constructed in a closed‐loop system, was used.OutcomesThe Fugl‐Meyer upper extremity score (the primary outcome), Action Research Arm Test, Motricity Index, Barthel Index, and modified Ashworth scale (the secondary outcomes) were administered by a blinded assessor at baseline, post–12th TS, post‐intervention, 1‐month, and 6‐month follow‐ups.ResultsNo significant differences between groups were found on the primary outcome at postintervention and follow‐up assessments. At 1‐month follow‐up, the innocuous group showed a small effect (partial η2 = 0.02) that was greater than that of the noxious group, but that effect was eliminated at 6 months. Both groups presented significant within‐group improvements over time (both P < .001).ConclusionsCombining noxious TS with traditional rehabilitation did not yield better short‐term or long‐term results than combining innocuous TS with traditional rehabilitation on UE functional recovery for individuals with acute stroke.Level of EvidenceII
Presentación de reporte de caso: adenocarcinoma de célula clara de uretra
Authors: González Nataly , Ramirez Yuly , Szelezsán Jose , Rojas Daniel 
Published: June 2017

ResumenEl adenocarcinoma de célula clara de uretra es un tumor agresivo, poco frecuente, que afecta predominantemente a mujeres adultas. Se presenta el caso de una paciente de 64 años con hematuria de larga data, en quien se diagnostica este tipo de tumor se realiza tratamiento quirúrgico y actualmente presenta evolución satisfactoria.
Targeted two-photon chemical apoptotic ablation of defined cell types in vivo
Authors: Hill Robert A. , Damisah Eyiyemisi C. , Chen Fuyi , Kwan Alex C. , Grutzendler Jaime 
Published: June 2017

AbstractA major bottleneck limiting understanding of mechanisms and consequences of cell death in complex organisms is the inability to induce and visualize this process with spatial and temporal precision in living animals. Here we report a technique termed two-photon chemical apoptotic targeted ablation (2Phatal) that uses focal illumination with a femtosecond-pulsed laser to bleach a nucleic acid-binding dye causing dose-dependent apoptosis of individual cells without collateral damage. Using 2Phatal, we achieve precise ablation of distinct populations of neurons, glia and pericytes in the mouse brain and in zebrafish. When combined with organelle-targeted fluorescent proteins and biosensors, we uncover previously unrecognized cell-type differences in patterns of apoptosis and associated dynamics of ribosomal disassembly, calcium overload and mitochondrial fission. 2Phatal provides a powerful and rapidly adoptable platform to investigate in vivo functional consequences and neural plasticity following cell death as well as apoptosis, cell clearance and tissue remodelling in diverse organs and species.
Observing electron localization in a dissociating H2+ molecule in real time
Authors: Xu H. , Li Zhichao , He Feng , Wang X. , Atia-Tul-Noor A. , Kielpinski D. , Sang R. T. , Litvinyuk I. V. 
Published: June 2017

AbstractDissociation of diatomic molecules with odd number of electrons always causes the unpaired electron to localize on one of the two resulting atomic fragments. In the simplest diatomic molecule H2+ dissociation yields a hydrogen atom and a proton with the sole electron ending up on one of the two nuclei. That is equivalent to breaking of a chemical bond—the most fundamental chemical process. Here we observe such electron localization in real time by performing a pump–probe experiment. We demonstrate that in H2+ electron localization is complete in just 15 fs when the molecule’s internuclear distance reaches 8 atomic units. The measurement is supported by a theoretical simulation based on numerical solution of the time-dependent Schrödinger equation. This observation advances our understanding of detailed dynamics of molecular dissociation.
Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape
Authors: Brodmann Maj , Dreier Roland F. , Broz Petr , Basler Marek 
Published: June 2017

AbstractFrancisella tularensis is an intracellular pathogen that causes the fatal zoonotic disease tularaemia. Critical for its pathogenesis is the ability of the phagocytosed bacteria to escape into the cell cytosol. For this, the bacteria use a non-canonical type VI secretion system (T6SS) encoded on the Francisella pathogenicity island (FPI). Here we show that in F. novicida T6SS assembly initiates at the bacterial poles both in vitro and within infected macrophages. T6SS dynamics and function depends on the general purpose ClpB unfoldase, which specifically colocalizes with contracted sheaths and is required for their disassembly. T6SS assembly depends on iglF, iglG, iglI and iglJ, whereas pdpC, pdpD, pdpE and anmK are dispensable. Importantly, strains lacking pdpC and pdpD are unable to escape from phagosome, activate AIM2 inflammasome or cause disease in mice. This suggests that PdpC and PdpD are T6SS effectors involved in phagosome rupture.
Lkb1 maintains Treg cell lineage identity
Authors: Wu Di , Luo Yuechen , Guo Wei , Niu Qing , Xue Ting , Yang Fei , Sun Xiaolei , Chen Song , Liu Yuanyuan , Liu Jingru , Sun Zhina , Zhao Chunxiao , Huang Huifang , Liao Fang , Han Zhongchao , Zhou Dongming , Yang Yongguang , Xu Guogang , Cheng Tao , Feng Xiaoming 
Published: June 2017

AbstractRegulatory T (Treg) cells are a distinct T-cell lineage characterized by sustained Foxp3 expression and potent suppressor function, but the upstream dominant factors that preserve Treg lineage-specific features are mostly unknown. Here, we show that Lkb1 maintains Treg cell lineage identity by stabilizing Foxp3 expression and enforcing suppressor function. Upon T-cell receptor (TCR) stimulation Lkb1 protein expression is upregulated in Treg cells but not in conventional T cells. Mice with Treg cell-specific deletion of Lkb1 develop a fatal early-onset autoimmune disease, with no Foxp3 expression in most Treg cells. Lkb1 stabilizes Foxp3 expression by preventing STAT4-mediated methylation of the conserved noncoding sequence 2 (CNS2) in the Foxp3 locus. Independent of maintaining Foxp3 expression, Lkb1 programs the expression of a wide spectrum of immunosuppressive genes, through mechanisms involving the augmentation of TGF-β signalling. These findings identify a critical function of Lkb1 in maintaining Treg cell lineage identity.
Adaptive self-assembly and induced-fit transformations of anion-binding metal-organic macrocycles
Authors: Zhang Ting , Zhou Li-Peng , Guo Xiao-Qing , Cai Li-Xuan , Sun Qing-Fu 
Published: June 2017

AbstractContainer-molecules are attractive to chemists due to their unique structural characteristics comparable to enzymes and receptors in nature. We report here a family of artificial self-assembled macrocyclic containers that feature induced-fit transformations in response to different anionic guests. Five metal-organic macrocycles with empirical formula of MnL2n (M=Metal; L=Ligand; n=3, 4, 5, 6, 7) are selectively obtained starting from one simple benzimidazole-based ligand and square-planar palladium(II) ions, either by direct anion-adaptive self-assembly or induced-fit transformations. Hydrogen-bonding interactions between the inner surface of the macrocycles and the anionic guests dictate the shape and size of the product. A comprehensive induced-fit transformation map across all the MnL2n species is drawn, with a representative reconstitution process from Pd7L14 to Pd3L6 traced in detail, revealing a gradual ring-shrinking mechanism. We envisage that these macrocyclic molecules with adjustable well-defined hydrogen-bonding pockets will find wide applications in molecular sensing or catalysis.
Probing ultrafast ππ*/nπ* internal conversion in organic chromophores via K-edge resonant absorption
Authors: Wolf T. J. A. , Myhre R. H. , Cryan J. P. , Coriani S. , Squibb R. J. , Battistoni A. , Berrah N. , Bostedt C. , Bucksbaum P. , Coslovich G. , Feifel R. , Gaffney K. J. , Grilj J. , Martinez T. J. , Miyabe S. , Moeller S. P. , Mucke M. , Natan A. , Obaid R. , Osipov T. , Plekan O. , Wang S. , Koch H. , Gühr M. 
Published: June 2017

AbstractMany photoinduced processes including photosynthesis and human vision happen in organic molecules and involve coupled femtosecond dynamics of nuclei and electrons. Organic molecules with heteroatoms often possess an important excited-state relaxation channel from an optically allowed ππ* to a dark nπ* state. The ππ*/nπ* internal conversion is difficult to investigate, as most spectroscopic methods are not exclusively sensitive to changes in the excited-state electronic structure. Here, we report achieving the required sensitivity by exploiting the element and site specificity of near-edge soft X-ray absorption spectroscopy. As a hole forms in the n orbital during ππ*/nπ* internal conversion, the absorption spectrum at the heteroatom K-edge exhibits an additional resonance. We demonstrate the concept using the nucleobase thymine at the oxygen K-edge, and unambiguously show that ππ*/nπ* internal conversion takes place within (60 ± 30) fs. High-level-coupled cluster calculations confirm the method’s impressive electronic structure sensitivity for excited-state investigations.
Predicting conformational ensembles and genome-wide transcription factor binding sites from DNA sequences
Authors: Andrabi Munazah , Hutchins Andrew Paul , Miranda-Saavedra Diego , Kono Hidetoshi , Nussinov Ruth , Mizuguchi Kenji , Ahmad Shandar 
Published: June 2017

AbstractDNA shape is emerging as an important determinant of transcription factor binding beyond just the DNA sequence. The only tool for large scale DNA shape estimates,DNAshapewas derived from Monte-Carlo simulations and predicts four broad and static DNA shape features,Propeller twist,Helical twist,Minor groove widthandRoll. The contributions of other shape features e.g.Shift,SlideandOpeningcannot be evaluated usingDNAshape. Here, we report a novel methodDynaSeq, which predicts molecular dynamics-derived ensembles of a more exhaustive set of DNA shape features. We compared theDNAshapeandDynaSeqpredictions for the common features and applied both to predict the genome-wide binding sites of 1312 TFs available from protein interaction quantification (PIQ) data. The results indicate a good agreement between the two methods for the common shape features and point to advantages in usingDynaSeq. Predictive models employing ensembles from individual conformational parameters revealed thatbase-pair opening -known to be important instrand separation -was the best predictor of transcription factor-binding sites (TFBS) followed by features employed byDNAshape. Of note, TFBS could be predicted not only from the features at the target motif sites, but also from those as far as 200 nucleotides away from the motif.
SLC4A11 depletion impairs NRF2 mediated antioxidant signaling and increases reactive oxygen species in human corneal endothelial cells during oxidative stress
Authors: Guha Sanjukta , Chaurasia Sunita , Ramachandran Charanya , Roy Sanhita 
Published: June 2017

AbstractCorneal endothelial dystrophy is a progressive disease with gradual loss of vision and characterized by degeneration and dysfunction of corneal endothelial cells. Mutations in SLC4A11, a Na+ dependent OH− transporter, cause congenital hereditary endothelial dystrophy (CHED) and Fuchs’ endothelial corneal dystrophy (FECD), the two most common forms of endothelial degeneration. Along with genetic factors, oxidative stress plays a role in pathogenesis of several corneal diseases. In this study we looked into the role of SLC4A11 in antioxidant stress response in human corneal endothelial cells (HCEnC). We found increased expression of SLC4A11 in presence of oxidative stress. Depletion of SLC4A11 using targeted siRNA, caused an increase in reactive oxygen species, cytochrome c, lowered mitochondrial membrane potential, and reduced cell viability during oxidative stress. Moreover, SLC4A11 was found to be necessary for NRF2 mediated antioxidant gene expression in HCEnC. On the other hand, over expression of SLC4A11 reduces reactive oxygen species levels and increases cell viability. Lastly, CHED tissue specimens show evidence of oxidative stress and reduced expression of NRF2. In conclusion, our data suggests a possible role of SLC4A11 in regulating oxidative stress, and might be responsible for both the etiology and treatment of corneal endothelial dystrophy.
Tracing the epidemic history of HIV-1 CRF01_AE clusters using near-complete genome sequences
Authors: Li Xingguang , Liu Haizhou , Liu Lu , Feng Yi , Kalish Marcia L. , Ho Simon Y. W. , Shao Yiming 
Published: June 2017

AbstractHuman immunodeficiency virus (HIV) has a number of circulating recombinant forms that are the product of recombination between different HIV subtypes. The first circulating recombinant form of HIV-1 to be identified was CRF01_AE, which originated in Central Africa and is now most prevalent in Southeast and East Asia. In this study, we investigated the timescale, evolutionary history, and population genetics of the HIV-1 CRF01_AE strains primarily responsible for the epidemic in Asia. A further aim of our study was to define and standardize the nomenclature and provide well-characterized reference sequences for the phylogenetic transmission clusters of CRF01_AE. We analysed a data set of 334 near-complete genome sequences from various risk groups, sampled between 1990 and 2011 from nine countries. Phylogenetic analyses of these sequences were performed using maximum likelihood and Bayesian methods. Our study confirms that the diversity of HIV-1 CRF01_AE originated in Central Africa in the mid-1970s, was introduced into Thailand between 1979 and 1982, and began expanding there shortly afterwards (1982–1984). Subsequently, multiple clusters significantly contributed to China’s HIV epidemic. A Bayesian skyline plot revealed the rapid expansion of CRF01_AE in China around 1999–2000. We identified at least eight different clusters of HIV-1 CRF01_AE formed by rapid expansion into different risk groups and geographic regions in China since the late 1980s.
Influence of Fermi arc states and double Weyl node on tunneling in a Dirac semimetal
Authors: Siu Zhuo Bin , Yesilyurt Can , Jalil Mansoor B. A. , Tan Seng Ghee 
Published: June 2017

AbstractMost theoretical studies of tunneling in Dirac and the closely related Weyl semimetals have modeled these materials as single Weyl nodes described by the three-dimensional Dirac equation $${\boldsymbol{H}}{\boldsymbol{=}}{{\boldsymbol{v}}}_{{\boldsymbol{f}}}\overrightarrow{{\boldsymbol{p}}}\cdot \overrightarrow{{\boldsymbol{\sigma }}}$$
H
=

v

f

p
→

⋅

σ
→

. The influence of scattering between the different valleys centered around different Weyl nodes, and the Fermi arc states which connect these nodes are hence not evident from these studies. In this work we study the tunneling in a thin film system of the Dirac semimetal Na3Bi consisting of a central segment with a gate potential, sandwiched between identical semi-infinite source and drain segments. The model Hamiltonian we use for Na3Bi gives, for each spin, two Weyl nodes separated in k-space symmetrically about k
z
= 0. The presence of a top and bottom surface in the thin film geometry results in the appearance of Fermi arc states and energy subbands. We show that (for each spin) the presence of two Weyl nodes and the Fermi arc states results in enhanced transmission oscillations, and finite transmission even when the energy falls within the bulk band gap in the central segment respectively. These features are not captured in single Weyl node models.
Human age and skin physiology shape diversity and abundance of Archaea on skin
Authors: Moissl-Eichinger Christine , Probst Alexander J. , Birarda Giovanni , Auerbach Anna , Koskinen Kaisa , Wolf Peter , Holman Hoi-Ying N. 
Published: June 2017

AbstractThe human skin microbiome acts as an important barrier protecting our body from pathogens and other environmental influences. Recent investigations have provided evidence that Archaea are a constant but highly variable component of the human skin microbiome, yet factors that determine their abundance changes are unknown. Here, we tested the hypothesis that the abundance of archaea on human skin is influenced by human age and skin physiology by quantitative PCR of 51 different skin samples taken from human subjects of various age. Our results reveal that archaea are more abundant in human subjects either older than 60 years or younger than 12 years as compared to middle-aged human subjects. These results, together with results obtained from spectroscopy analysis, allowed us gain first insights into a potential link of lower sebum levels and lipid content and thus reduced skin moisture with an increase in archaeal signatures. Amplicon sequencing of selected samples revealed the prevalence of specific eury- and mainly thaumarchaeal taxa, represented by a core archaeome of the human skin.
Targeting Coronaviral Replication and Cellular JAK2 Mediated Dominant NF-κB Activation for Comprehensive and Ultimate Inhibition of Coronaviral Activity
Authors: Yang Cheng-Wei , Lee Yue-Zhi , Hsu Hsing-Yu , Shih Chuan , Chao Yu-Sheng , Chang Hwan-You , Lee Shiow-Ju 
Published: June 2017

AbstractTylophorine-based compounds exert broad spectral, potent inhibition of coronaviruses. NF-κB activation is a common pro-inflammatory response of host cells to viral infection. The aims of this study were to (i) find an effective combination treatment for coronaviral infections through targeting of the virus per se and cellular NF-κB activity; and (ii) to study the underling mechanisms. We found that tylophorine-based compounds target the TGEV viral RNA and effectively inhibit TGEV replication. NF-κB inhibition also leads to anti-TGEV replication. NF-κB activation induced by TGEV infection was found to be associated with two convergent pathways, IKK-2_IκBα/p65 and JAK2 mediated p65 phosphorylation, in swine testicular cells. JAK2 inhibition either by CYT387 (a JAK family inhibitor) or by silencing JAK2-expression revealed a dominant JAK2 mediated p65 phosphorylation pathway for NF-κB activation and resulted in NF-κB inhibition, which overrode the IκBα regulation via the IKK-2. Finally, tylophorine-based compounds work cooperatively with CYT387 to impart comprehensive anti-TGEV activities. The combination treatment, wherein a tylophorine compound targets TGEV and a JAK2 inhibitor blocks the alternative dominant NF-κB activation mediated by JAK2, is more effective and comprehensive than either one alone and constitutes a feasible approach for the treatment of SARS-CoV or MERS-CoV.
Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
Authors: Metwally Nahla Galal , Tilly Ann-Kathrin , Lubiana Pedro , Roth Lisa K. , Dörpinghaus Michael , Lorenzen Stephan , Schuldt Kathrin , Witt Susanne , Bachmann Anna , Tidow Henning , Gutsmann Thomas , Burmester Thorsten , Roeder Thomas , Tannich Egbert , Bruchhaus Iris 
Published: June 2017

AbstractThe ability of the parasitePlasmodium falciparumto evade the immune system and be sequestered within human small blood vessels is responsible for severe forms of malaria. The sequestration depends on the interaction between human endothelial receptors andP. falciparumerythrocyte membrane protein 1 (PfEMP1) exposed on the surface of the infected erythrocytes (IEs). In this study, the transcriptomes of parasite populations enriched for parasites that bind to human P-selectin, E-selectin, CD9 and CD151 receptors were analysed. IT4_var02 and IT4_var07 were specifically expressed in IT4 parasite populations enriched for P-selectin-binding parasites; eightvargenes (IT4_var02/07/09/13/17/41/44/64) were specifically expressed in isolate populations enriched for CD9-binding parasites. Interestingly, IT4 parasite populations enriched for E-selectin- and CD151-binding parasites showed identical expression profiles to those of a parasite population exposed to wild-type CHO-745 cells. The same phenomenon was observed for the 3D7 isolate population enriched for binding to P-selectin, E-selectin, CD9 and CD151. This implies that the corresponding ligands for these receptors have either weak binding capacity or do not exist on the IE surface. Conclusively, this work expanded our understanding ofP. falciparumadhesive interactions, through the identification ofvartranscripts that are enriched within the selected parasite populations.