bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post‐traumatic growth and its explanatory factors in burn patients: A systematic review
Authors: Kazemi‐Sufi Siamak , Bagheri Ayub , Mazhari Seyed Amirhossein , Farhadi Bahar , Alizadeh‐Otaghvar Hamidreza , Zaboli Mahdiabadi Morteza , Najafi Masoomeh , Farzan Ramyar , Salehi Reza 
Published: October 2024

AbstractA few studies have examined post‐traumatic growth (PTG) after burns, a relatively new area of research. To understand how to promote PTG in burn survivors, we need to know how PTG and coping are presented, how they change over time, and the components that influence development. Based on the criteria of the PRISMA statement, we conducted our initial search on 1 February 2023. This study systematically reviewed relevant studies in five PubMed, Web of Science, Google Scholar, Scopus and Science Direct search databases. The search used the following keywords: Post‐traumatic Growth, Psychological Growth, Post‐traumatic Growth, Psychological, PTG, Burns and Burn. The two researchers evaluated the quality of the included studies using a 20‐item tool called the appraisal tool for cross‐sectional studies (AXIS tool). A total of 1396 burn patients participated in 12 studies. Among the studies included in this systematic review, seven were cross‐sectional, and five were cohort. The average age of the patients in this study was 35.77 (SD = 10.19). Among the participants, 52.4% were men. The total body surface area (TBSA) percentage in these patients was 32.77 (SD = 16.42). The mean of PTG inventory (PTGI) was 54.08 (SD = 20.46) out of 105, which is a moderate level. Factors such as coping strategies, perceived social support, TBSA, religion/spirituality and severity of burn are positive factors for PTG in burn survivors. Also, factors such as depression, post‐traumatic stress disorder and physical and mental recovery are negative factors for PTG in burn survivors. These factors can be improved through targeted intervention strategies, including overall function, quality of life, social support, optimism, hope and new opportunities. There is a need for further research in several areas related to growth, intervention and measurement.
Retention processed placental membrane versus standard of care in treating diabetic foot ulcers
Authors: Frykberg Robert G. , Tunyiswa Zwelithini , Weston Wendy W. 
Published: October 2024

AbstractDiabetic foot ulcers (DFUs) are a severe complication for diabetic patients, significantly impacting patient quality of life and healthcare system efficiency. Traditional standard of care (SOC) treatments are inadequate for many patients, necessitating the use of advanced wound care products, such as human placental membranes. We studied a real‐world population of large, hard‐to‐heal and complicated wounds, otherwise under‐studied in the wound care literature. To this end, we conducted a retrospective cohort analysis to compare the effectiveness of a human placental amnion/chorion membrane product using retention‐based processing (RE‐AC) and SOC in managing chronic DFUs. During the study period of September 2021 through April 2024, we collected retrospective observational data from electronic health records of 21 patients treated with RE‐AC at three outpatient wound care centres. Additionally, 21 control SOC patients were matched from a wound registry using Coarsened Exact Matching. Patients were categorized into two cohorts based on whether they received RE‐AC or SOC. Key metrics included wound size progression and wound closure. The analysis employed Bayesian regression and Hurdle Gamma Analysis of Covariance models. Despite their rather large size (average of 13.8 cm2), our results indicated that RE‐AC achieved almost a 10% higher expected wound closure rate compared to SOC at 12 weeks (8.53% [credible interval: 5.60%–10.7%]). Further, for wounds that did not close, RE‐AC resulted in a 93.6% (credible interval: 147.7%–41.6) improvement in expected Percent Area Reduction over the SOC group at 12 weeks. We noted that on average, SOC wounds stalled or grew larger. In terms of a risk ratio comparing the study group with SOC, we found a 52% benefit in the RE‐AC group (RR = 1.52). The findings suggest that even with larger DFUs, R‐AC is superior to SOC for wound closure and expected Percent Area Reduction by 12 weeks. This benefit likely leads to reduced treatment costs, optimized resource utilization and improved outcomes in the DFU patient population; ultimately resulting in improved patient care.
Family Resilience and Its Influencing Factors in Patients With Cancer and Their Family Members: A Systematic Review
Authors: Qiu Xiaoke , Mao Jiayu , Wang Can , Yang Xueli , Li Qiuping 
Published: October 2024

ABSTRACTAimsThis review aims to explore factors influencing family resilience in families providing care for patients with cancer and to provide suggestions for future research directions.MethodsSix electronic databases were searched including Web of Science, CINAHL, EMBASE, PsycINFO, PubMed and CNKI from their inception to December 2023. The article reference lists were also manually searched. The Mixed Method Appraisal Tool was used to assess the included studies in this review. The 27‐item checklist Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) statement was followed to report this review.ResultsTwenty‐one studies from six online databases reported that either individual or family factors have effects on family resilience and were described into five clusters on the basis of the Walsh model of family resilience, including demographic and clinical factors, personal strengths and resources, family stressors, family resilient coping processes and family resilient adaptation outcomes.ConclusionFamily resilience in cancer families plays a pivotal role in coping with family stressors and facilitating positive outcomes through domains of coping. Future researches need to explore factors related to family resilience from dyadic perspectives and to establish multidisciplinary intervention strategies for developing levels of family resilience in cancer families.Trial RegistrationPROSPERO: CRD42024535349
Diversity of lice and flea‐ and lice‐borne pathogens in free‐ranging dogs in Uzbekistan
Authors: Panait Luciana Cătălina , Safarov Alisher , Deak Georgiana , Ionică Angela Monica , Mihalca Andrei Daniel 
Published: October 2024

AbstractVector‐borne diseases pose a significant threat to human and animal health worldwide, with arthropods, including fleas and lice, acting as key vectors for transmitting various pathogens. In Uzbekistan, where millions of domestic dogs coexist with humans, the diversity of vector‐borne pathogens carried by ectoparasites remains largely unexplored. This study aimed to investigate the diversity and prevalence of lice and pathogens carried by fleas and lice collected from domestic dogs in Uzbekistan. Ectoparasites were collected from 77 dogs across five regions and subjected to morphological identification and molecular analysis. The 47 lice collected were identified as Trichodectes canis Fabricius, 1780 (Psocodea: Trichodectidae), Linognathus africanus Kellogg and Paine, 1911 (Psocodea: Linognathidae) and Bovicola bovis Linnaeus, 1758 (Phthiraptera: Trichodectidae), alongside with 197 fleas. Notably, L. africanus was detected for the first time on dogs worldwide. Molecular screening detected Rickettsia spp. in 27.9% fleas and 44.7% lice, Dipylidium caninum Linnaeus, 1758 (Cyclophyllidea: Dipylidiidae) in 1.5% of fleas and Bartonella spp. in 0.5% of fleas. Sequencing confirmed Rickettsia asembonensisMaina et al., 2016 (Rickettsiales: Rickettsiaceae), Rickettsia helvetica Beati et al., 1993 (Rickettsiales: Rickettsiaceae), Rickettsia monacensis Simser et al., 2019 (Rickettsiales: Rickettsiaceae), D. caninum and Bartonella clarridgeiae Lawson and Collins, 1996 (Hyphomicrobiales: Bartonellaceae). These findings highlight the potential role of dogs as reservoirs for vector‐borne pathogens and emphasises the need for further research to inform effective disease control strategies.
The Different Concentrations of Applied Exogenous Sugars Widely Influence the Specificity, Significance and Physiological Relevance of Study Outcomings
Authors: Wang Yi‐Bo , Shi Ya‐Na , Bao Qin‐Xin , Mu Xin‐Rong , Yu Fu‐Huan , Zou Ya‐Li , Meng Lai‐Sheng 
Published: October 2024

ABSTRACTPlant growth and development are governed via signal networks that connect inputs from nutrient status, hormone signals, and environmental cues. Substantial researches have indicated a pivotal role of sugars as signalling molecules in plants that integrate external environmental cues and other nutrients with intrinsic developmental programmes regulated via multiple plant hormones. Therefore, plant growth and development are controlled through complication signalling networks. However, in many studies, to obtain more obviously experimental findings, excess concentrations of applied exogenous sugars have aggravated the complexity of this signalling networks. Once researchers underestimate this complexity, a series of contradictory or contrasting findings will be generated. More importantly, in terms of these contradictory findings, more contradictory study outcomings are derived. In this review, we carefully analyze some reports, and find that these reports have confused or neglected that the sugar‐antagonism of ethylene signalling is specific or conditional. As a result, many contradictory conclusions are generated, which will in turn misdirect the scientific community.
The extent of public awareness and use of the Global Solar UV Index as a worldwide health promotion instrument to improve sun protection: A systematic review and meta‐analysis
Authors: Kaiser Isabelle , Pfahlberg Annette B. , Lehmann Maria , Buchta Esther , Uter Wolfgang , Gefeller Olaf 
Published: October 2024

AbstractThirty years ago, the Global Solar UV Index (UVI) has been introduced as a health promotion instrument to improve sun protection. We assessed systematically global levels of awareness and use of the UVI as a prerequisite for the preventive effectiveness of this public health tool. We conducted a comprehensive literature search across 10 databases, including PubMed, Scopus and Web of Science Core Collection, as well as clinical trial registries and gray literature databases. The risk of bias of studies was evaluated using the Joanna Briggs Institute checklist for prevalence studies. In addition to narrative and descriptive analysis, we performed meta‐analyses with geographical subgroup analyses to statistically summarize the results. In total, we identified 40 publications from 39 different studies across multiple global regions. However, the number of studies in the analyses varies depending on the outcome. The results, especially the awareness of the UVI, were largely dependent on the specific geographical location of the studies. While the prevalence of awareness of the UVI is high among Australian populations, there is considerable variability in levels of awareness across other global regions. At the same time, the use of the UVI is at a low level across all regions, demonstrating the need for enhanced dissemination of knowledge about the perils associated with ultraviolet radiation and the advantages of using the UVI.
Catalase regulation during plant‐virus‐vector interaction
Authors: Sharma Rohit , Pandey Saurabh , Prasad Manoj , Prasad Ashish 
Published: October 2024

Key messagePlant‐virus‐host interaction is a complex process involving several players. A constant arms race between the hosts and viruses has led to their co‐evolution. Reactive oxygen species (ROS) are important signaling molecules that regulate plant growth, development, and stress responses. Barley yellow dwarf virus (BYDV) has a wide host range and infects several plant species such as barley, rice, oats, wheat, etc. A recent study by Tian et al. (2024) has highlighted that the movement protein (MP) of BYDV is involved in manipulation of the host ROS pathway to promote viral multiplication as well as transmission. The findings display the multifaceted role of a viral protein that is otherwise involved in movement. The limited coding ability of viruses is compensated by their proteins having multiple roles in the modulation of several different host molecular pathways. This is one of the key reasons for viruses being successful pathogens despite their limited coding ability.
Lectin Functionalised Iron Magnetic Nanoparticle‐Based Sperm Selection: A Potential Technique to Improve Bull Sperm Quality In Vitro
Authors: Paul Nilendu , Kumaresan A. , Talluri T. R. , Raval Kathan , Elango Kamaraj , Pradeep Nag B. S. , Duraisamy Rajendran , Manimaran A. 
Published: October 2024

ABSTRACTPremature acrosomal exocytosis in cryopreserved semen is one of the reasons attributed to low fertility among livestock. In the present study, we attempted to enhance the cryopreserved semen quality by selective removal of acrosome‐reacted spermatozoa using FITC‐PNA conjugated iron magnetic nanoparticles (MNPs). Further, the effect of nano purification on other sperm functional attributes was also assessed. Iron MNPs were prepared using co‐precipitation method and dextran‐coated MNPs were conjugated with FITC‐PNA (0.04 mg/mL). A preliminary experiment was conducted to standardise the dose of FITC‐PNA conjugated iron MNPs (0.02, 0.05, 0.1, 0.15, 0.2, 0.4 and 0.6 mg). Among the different doses used, 0.6 mg FITC‐PNA conjugated iron MNPs significantly (p < 0.05) removed higher acrosomal reacted spermatozoa from the semen, and therefore, this dose was used in further experiments. Cryopreserved semen from Holstein Friesian breeding bulls (n = 6) were thawed and washed using Sperm‐TALP to remove residual extender. Washed spermatozoa (2 × 106) were exposed to 0.6 mg of FITC‐PNA conjugated iron MNPs for 10 min at 37°C. The nano purified semen was assessed for various vital sperm parameters viz., viability, intracellular calcium, apoptosis, mitochondrial ROS and mitochondrial membrane potential using flow cytometry. We found that nanopurification using FITC‐PNA conjugated iron MNPs significantly (p < 0.05) improved the sperm quality. The proportion of viable non‐apoptotic spermatozoa with low intracellular calcium levels was significantly (p < 0.05) enriched in nano purified semen. Nano purification did not affect sperm mitochondrial membrane potential and ROS production. In conclusion, these preliminary findings indicate that FITC‐PNA coated iron MNPs effectively removed acrosome reacted spermatozoa and significantly improved sperm functional attributes in the purified fraction.
Language, Cultural Identity, and Spirituality —A Case Study of the Representation of Minority Ethnic Subjects in Chinese Ethnographic Documentary
Authors: Tang Ruohan , Zou Yi 
Published: October 2024

AbstractSince the 1990s, there has been a surge in ethnographic films by Han directors in China, highlighting the complex interplay of power dynamics between these filmmakers and the minority communities they portray. This study examines these dynamics through the lens of spirituality and cultural representation, focusing on the film “Ashiq: The Last Troubadour.” A case study and interviews with the director, Liu Xiangchen, reveal an essentialist portrayal of the Uygur Ashiq that emphasises their spiritual and cultural identity on Uighur language chant, inadvertently reinforcing Han dominance. The analysis suggests that while the director's intentions reflect a critical self‐awareness, the film perpetuates the marginalisation of the Ashiq. We argue that ethnographic cinema should offer a more nuanced depiction of ethnic minorities and their minority language to challenge social injustices further and promote inclusivity in China's diverse linguistic and cultural landscape.
Sharp propagation of chaos for the ensemble Langevin sampler
Authors: Vaes U. 
Published: October 2024

AbstractThe aim of this paper is to revisit propagation of chaos for a Langevin‐type interacting particle system recently proposed as a method to sample probability measures. The interacting particle system we consider coincides, in the setting of a log‐quadratic target distribution, with the ensemble Kalman sampler [SIAM J. Appl. Dyn. Syst. 19 (2020), no. 1, 412–441], for which propagation of chaos was first proved by Ding and Li in [SIAM J. Math. Anal. 53 (2021), no. 2, 1546–1578]. Like these authors, we prove propagation of chaos with an approach based on a synchronous coupling, as in Sznitman's classical argument. Instead of relying on a boostrapping argument, however, we use a technique based on stopping times in order to handle the presence of the empirical covariance in the coefficients of the dynamics. The use of stopping times to handle the lack of global Lipschitz continuity in the coefficients of stochastic dynamics originates from numerical analysis [SIAM J. Numer. Anal. 40 (2002), no. 3, 1041–1063] and was recently employed to prove mean‐field limits for consensus‐based optimization and related interacting particle systems [arXiv:2312.07373, 2023; Math. Models Methods Appl. Sci. 33 (2023), no. 2, 289–339]. In the context of ensemble Langevin sampling, this technique enables proving pathwise propagation of chaos with optimal rate, whereas previous results were optimal only up to a positive .
Shining a light on the impact of antifungals on
Aspergillus fumigatus
subcellular dynamics through fluorescence imaging
Authors: Storer I. S. R. , Sastré-Velásquez L. E. , Easter T. , Mertens B. , Dallemulle A. , Bottery M. , Tank R. , Offterdinger M. , Bromley M. J. , van Rhijn N. , Gsaller F. 
Published: October 2024

ABSTRACT

Fluorescent proteins (FPs) are indispensable tools used for molecular imaging, single-cell dynamics, imaging in infection models, and more. However, next-generation FPs have yet to be characterized in
Aspergillus
. Here, we characterize 18 FPs in the pathogenic filamentous fungus
Aspergillus fumigatus
spanning the visible light spectrum. We report on
in vivo
FP brightness in hyphal and spore morphotypes and show how a fluoropyrimidine-based selection system can be used to iteratively introduce four distinct FPs enabling the simultaneous visualization of the cell membrane, mitochondria, peroxisomes, and vacuoles. Using this strain, we describe and compare the dynamic responses of organelles to stresses induced by voriconazole, amphotericin B, and the novel antifungal drugs olorofim and manogepix. The expansion to the fluorescent genetic toolbox will overcome boundaries in research applications that involve fluorescence imaging in filamentous fungi.
The puzzle of two tandem acyl-CoA ligases of
Pseudomonas putida
F1
Authors: Dong Huijuan , Chen Bo , Wang Haihong , Cronan John E. 
Published: October 2024

ABSTRACT

The
Pseudomonas putida
F1 genome and those of many other pseudomonads contain two tandem genes encoding acyl-CoA ligases Pput_1340 (
fadD1
) and Pput_1339 (
fadD2
) with Pput_1339 (
fadD2
) being the upstream gene. The
fadD
designation was assigned when both genes were found to complement the growth of an
Escherichia coli
acyl-CoA synthetase
fadD
deletion strain with oleic acid as sole carbon source. Site-directed mutagenesis showed that residues of the ATP/AMP domain required for function of
E. coli
FadD were also essential for full function of FadD1 and FadD2. Growth of the constructed ∆
fadD1
, ∆
fadD2,
and ∆
fadD1
∆
fadD2
strains was tested in minimal medium with different chain length fatty acids as sole carbon sources. Lack of FadD1 significantly retarded growth with different chain length fatty acids and lack of both FadD1 and FadD2 further retarded growth. Derivatives of the ∆
fabA
∆
desA
unsaturated fatty acid auxotrophic strain carrying a deletion of either ∆
fadD1
or ∆
fadD2
were constructed. Growth of the ∆
fabA
∆
desA
∆
fadD1
strain was very weak, whereas the ∆
fabA
∆
desA
∆
fadD2
strain grew as well as the ∆
fabA
∆
desA
parent strain. Overexpression of either
fadD1
or
fadD2
restored growth of the ∆
fabA
∆
desA
∆
fadD1
strain with
fadD2
overexpression having a greater effect than
fadD1
overexpression. The ∆
fadD1
or ∆
fadD2
genes are cotranscribed although the expression level of
fadD1
is much higher than that of
fadD2
. This is attributed to a
fadD1
promoter located within the upstream FadD2 coding sequence.

IMPORTANCE

Pseudomonas
bacteria demonstrate a great deal of metabolic diversity and consequently colonize a wide range of ecological niches. A characteristic of these bacteria is a pair of genes in tandem annotated as acyl-CoA ligases involved in fatty acid degradation. The
Pseudomonas putida
F1 genome is annotated as having at least nine genes encoding acyl-CoA ligases which are scattered around the chromosome excepting the tandem pair. Since similar tandem pairs are found in other pseudomonads, we have constructed and characterized deletion mutants of the tandem ligases. We report that the encoded proteins are authentic acyl-CoA ligases involved in fatty acid degradation.
The vibriophage-encoded inhibitor OrbA abrogates BREX-mediated defense through the ATPase BrxC
Authors: Oshiro Reid T. , Dunham Drew T. , Seed Kimberley D. 
Published: October 2024

ABSTRACT

Bacteria and phages are locked in a co-evolutionary arms race where each entity evolves mechanisms to restrict the proliferation of the other. Phage-encoded defense inhibitors have proven powerful tools to interrogate how defense systems function. A relatively common defense system is BREX (bacteriophage exclusion); however, how BREX functions to restrict phage infection remains poorly understood. A BREX system encoded by the
s
ulfametho
x
azole and
t
rimethoprim (SXT) integrative and conjugative element,
Vch
Ind5, was recently identified in
Vibrio cholerae
, the causative agent of the diarrheal disease cholera. The lytic phage ICP1 (
I
nternational Centre for Diarrhoeal Disease Research, Bangladesh
c
holera
p
hage
1
) that co-circulates with
V. cholerae
encodes the BREX-inhibitor OrbA, but how OrbA inhibits BREX is unclear. Here, we determine that OrbA inhibits BREX using a unique mechanism from known BREX inhibitors by directly binding to the BREX component BrxC. BrxC has a functional ATPase domain that, when mutated, not only disrupts BrxC function but also alters how BrxC multimerizes. Furthermore, we find that OrbA binding disrupts BrxC-BrxC interactions. We determine that OrbA cannot bind BrxC encoded by the distantly related BREX system encoded by the aSXT
Vch
Ban9, and thus fails to inhibit this BREX system that also circulates in epidemic
V. cholerae
. Lastly, we find that homologs of the
Vch
Ind5 BrxC are more diverse than the homologs of the
Vch
Ban9 BrxC. These data provide new insight into the function of the BrxC ATPase and highlight how phage-encoded inhibitors can disrupt phage defense systems using different mechanisms.

IMPORTANCE

With renewed interest in phage therapy to combat antibiotic-resistant pathogens, understanding the mechanisms bacteria use to defend themselves against phages and the counter-strategies phages evolve to inhibit defenses is paramount. Bacteriophage exclusion (BREX) is a common defense system with few known inhibitors. Here, we probe how the vibriophage-encoded inhibitor OrbA inhibits the BREX system of
Vibrio cholerae
, the causative agent of the diarrheal disease cholera. By interrogating OrbA function, we have begun to understand the importance and function of a BREX component. Our results demonstrate the importance of identifying inhibitors against defense systems, as they are powerful tools for dissecting defense activity and can inform strategies to increase the efficacy of some phage therapies.
Draft genome sequencing of multidrug-resistant
Pseudomonas asiatica
strains isolated from dairy cows with clinical mastitis and their farm environment
Authors: Sultana Taniya , Siddique Naim , Rahaman Monira , Rahman Md. Morshedur , Rahman ANM Aminoor , Talukder Anup Kumar , Das Ziban Chandra , Hoque M. Nazmul 
Published: October 2024

ABSTRACT

We report, for the first time, the draft genomes of four multidrug-resistant
Pseudomonas asiatica
strains isolated from milk (2M1), feces (2F1 and 2F2), and farm soil (2S1) samples of dairy cows with clinical mastitis. The assembled genomes of these strains were 5.7 Mbp, 62.8% G + C content, and 50× genome coverage.