bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Fluidized Bed Bioartificial Liver in Treating Fulminant Hepatic Failure in Pigs: A Metabolomics Study
Authors: Zhou Pengcheng , Shao Li , Zhao Lifu , Lv Guoliang , Pan Xiaoping , Zhang Anye , Li Jianzhou , Zhou Ning , Chen Deying , Li Lanjuan 
Published: May 2016

AbstractBioartificial livers may act as a promising therapy for fulminant hepatic failure (FHF) with better accessibility and less injury compared to orthotopic liver transplantation. This study aims to evaluate the efficacy and safety of a fluidized bed bioartificial liver (FBBAL) and to explore its therapeutic mechanisms based on metabolomics. FHF was induced by D-galactosamine. Eighteen hours later, pigs were treated with an FBBAL containing encapsulated primary porcine hepatocytes (B group), with a sham FBBAL (containing cell-free capsules, S group) or with only intensive care (C group) for 6 h. Serum samples were assayed using ultra-performance liquid chromatography-mass spectrometry. The difference in survival time (51.6 ± 7.9 h vs. 49.3 ± 6.6 h) and serum metabolome was negligible between the S and C groups, whereas FBBAL treatment significantly prolonged survival time (70.4 ± 11.5h, P < 0.01) and perturbed the serum metabolome, resulting in a marked decrease in phosphatidylcholines, lysophosphatidylcholines, sphingomyelinase, and fatty acids and an increase in conjugated bile acids. The FBBAL exhibits some liver functions and may exert its therapeutic effect by altering the serum metabolome of FHF pigs. Moreover, alginate–chitosan capsules have less influence on serum metabolites. Nevertheless, the alterations were not universally beneficial, revealing that much should be done to improve the FBBAL.
Subtle reproductive impairment through nitric oxide-mediated mechanisms in sea urchins from an area affected by harmful algal blooms
Authors: Migliaccio Oriana , Castellano Immacolata , Di Cioccio Davide , Tedeschi Gabriella , Negri Armando , Cirino Paola , Romano Giovanna , Zingone Adriana , Palumbo Anna 
Published: May 2016

AbstractThe health of the sea urchin Paracentrotus lividus, a key species in the Mediterranean Sea, is menaced by several pressures in coastal environments. Here, we aimed at assessing the reproductive ability of apparently healthy P. lividus population in a marine protected area affected by toxic blooms of Ostreospsis cf. ovata. Wide-ranging analyses were performed in animals collected prior to and during the bloom, as well as at several times thereafter, during the reproductive season. Adults showed a low fertilization rate, along with high nitric oxide (NO) levels in the gonads and the nitration of the major yolk protein toposome, which is an important player in sea urchin development. Serious developmental anomalies were observed in the progeny, which persist several months after the bloom. NO levels were high in the different developmental stages, which also showed variations in the transcription of several genes that were found to be directly or indirectly modulated by NO. These results highlight subtle but important reproductive flaws transmitted from the female gonads to the offspring with the NO involvement. Despite a recovery along time after the bloom, insidious damages can be envisaged in the local sea urchin population, with possible reverberation on the whole benthic system.
In actio optophysiological analyses reveal functional diversification of dopaminergic neurons in the nematode C. elegans
Authors: Tanimoto Yuki , Zheng Ying Grace , Fei Xianfeng , Fujie Yukako , Hashimoto Koichi , Kimura Koutarou D. 
Published: May 2016

AbstractMany neuronal groups such as dopamine-releasing (dopaminergic) neurons are functionally divergent, although the details of such divergence are not well understood. Dopamine in the nematode Caenorhabditis elegans modulates various neural functions and is released from four left-right pairs of neurons. The terminal identities of these dopaminergic neurons are regulated by the same genetic program, and previous studies have suggested that they are functionally redundant. In this study, however, we show functional divergence within the dopaminergic neurons of C. elegans. Because dopaminergic neurons of the animals were supposedly activated by mechanical stimulus upon entry into a lawn of their food bacteria, we developed a novel integrated microscope system that can auto-track a freely-moving (in actio) C. elegans to individually monitor and stimulate the neuronal activities of multiple neurons. We found that only head-dorsal pair of dopaminergic neurons (CEPD), but not head-ventral or posterior pairs, were preferentially activated upon food entry. In addition, the optogenetic activation of CEPD neurons alone exhibited effects similar to those observed upon food entry. Thus, our results demonstrated functional divergence in the genetically similar dopaminergic neurons, which may provide a new entry point toward understanding functional diversity of neurons beyond genetic terminal identification.
Functional analysis of the N-terminal basic motif of a eukaryotic satellite RNA virus capsid protein in replication and packaging
Authors: Sivanandam Venkatesh , Mathews Deborah , Garmann Rees , Erdemci-Tandogan Gonca , Zandi Roya , Rao A. L. N. 
Published: May 2016

AbstractEfficient replication and assembly of virus particles are integral to the establishment of infection. In addition to the primary role of the capsid protein (CP) in encapsidating the RNA progeny, experimental evidence on positive sense single-stranded RNA viruses suggests that the CP also regulates RNA synthesis. Here, we demonstrate that replication of Satellite tobacco mosaic virus (STMV) is controlled by the cooperative interaction between STMV CP and the helper virus (HV) Tobacco mosaic virus (TMV) replicase. We identified that the STMV CP-HV replicase interaction requires a positively charged residue at the third position (3R) in the N-terminal 13 amino acid (aa) motif. Far-Northwestern blotting showed that STMV CP promotes binding between HV-replicase and STMV RNA. An STMV CP variant having an arginine to alanine substitution at position 3 in the N-terminal 13aa motif abolished replicase-CP binding. The N-terminal 13aa motif of the CP bearing alanine substitutions for positively charged residues located at positions 5, 7, 10 and 11 are defective in packaging full-length STMV, but can package a truncated STMV RNA lacking the 3′ terminal 150 nt region. These findings provide insights into the mechanism underlying the regulation of STMV replication and packaging.
Ionic Conduction in Lithium Ion Battery Composite Electrode Governs Cross-sectional Reaction Distribution
Authors: Orikasa Yuki , Gogyo Yuma , Yamashige Hisao , Katayama Misaki , Chen Kezheng , Mori Takuya , Yamamoto Kentaro , Masese Titus , Inada Yasuhiro , Ohta Toshiaki , Siroma Zyun , Kato Shiro , Kinoshita Hajime , Arai Hajime , Ogumi Zempachi , Uchimoto Yoshiharu 
Published: May 2016

AbstractComposite electrodes containing active materials, carbon and binder are widely used in lithium-ion batteries. Since the electrode reaction occurs preferentially in regions with lower resistance, reaction distribution can be happened within composite electrodes. We investigate the relationship between the reaction distribution with depth direction and electronic/ionic conductivity in composite electrodes with changing electrode porosities. Two dimensional X-ray absorption spectroscopy shows that the reaction distribution is happened in lower porosity electrodes. Our developed 6-probe method can measure electronic/ionic conductivity in composite electrodes. The ionic conductivity is decreased for lower porosity electrodes, which governs the reaction distribution of composite electrodes and their performances.
Genetically diverse CC-founder mouse strains replicate the human influenza gene expression signature
Authors: Elbahesh Husni , Schughart Klaus 
Published: May 2016

AbstractInfluenza A viruses (IAV) are zoonotic pathogens that pose a major threat to human and animal health. Influenza virus disease severity is influenced by viral virulence factors as well as individual differences in host response. We analyzed gene expression changes in the blood of infected mice using a previously defined set of signature genes that was derived from changes in the blood transcriptome of IAV-infected human volunteers. We found that the human signature was reproduced well in the founder strains of the Collaborative Cross (CC) mice, thus demonstrating the relevance and importance of mouse experimental model systems for studying human influenza disease.
Nontyphoidal Salmonellosis, Human Immunodeficiency Virus Infection, and Ischemic Stroke
Authors: Piggott Damani A. , Carroll Karen C. , Lim Michael , Melia Michael T. 
Published: May 2016

Abstract
Nontyphoidal Salmonella infection and stroke are major causes of morbidity and mortality worldwide, with increased risk in the human immunodeficiency virus (HIV)-infected population. We report a rare case of ischemic stroke associated with Salmonella enteritidis subdural empyema in an older HIV-infected patient with multimorbidity, despite surgery and treatment with susceptible antimicrobial drugs.
Causal link between insulin‐like growth factor 1 and growth in nestlings of a wild passerine bird
Authors: Lodjak Jaanis , Mägi Marko , Sild Elin , Mänd Raivo 
Published: May 2016

Summary

Life history of animals is strongly linked with the variability in postnatal growth rates, because the ability to achieve necessary body mass early in life is directly connected with the ability to survive and reproduce later in life. Surprisingly, little is known about the physiological mechanisms connecting food availability with the variability of growth in wild animals.

We used a hormonal injection treatment in free‐living nestlings of the pied flycatcher (Ficedula hypoleuca). In doing this, we aimed to see how their plasma insulin‐like growth factor 1 (IGF‐1) levels and its mediation of growth rate vary in response to administration of exogenous IGF‐1 during the early period of postnatal development.

We showed that the levels of IGF‐1 as well as growth rate and body size, in terms of body mass and tarsus length, were significantly higher in IGF‐1‐injected nestlings, compared with those in the control group. These differences became especially pronounced from day 7 post‐hatch onwards during the 13‐day nestling period. Additionally, the proportional investment into growth was more profoundly downregulated in IGF‐1‐injected nestlings at the end of the nestling period, which achieved fledging size earlier, compared with control nestlings.

Our results are the first to highlight IGF‐1 as the causal link between growth conditions and variability of growth rate and body size in wild vertebrates. Interestingly, the change in IGF‐1 levels also showed a plausible, distinct adaptive age‐ and physical maturity‐dependent pattern in nestlings to match the benefits gained by them when investing in growth, with the cost endured during the early days of postnatal development to maximize their fitness. Therefore, this mechanism might be among those mediating the maintenance of genetic variability in growth rates in populations, presumably regardless of the presence of strong directional selection acting upon them.

A Lay Summary is available for this article.
Subjectification in Times of Indebtedness and Neoliberal/Austerity Urbanism
Authors: Di Feliciantonio Cesare 
Published: May 2016

AbstractHow can we analyse the (re)emergence of squatting in relation to the current housing crisis in Italy? Centred on the case of Rome, the paper theorizes this return as resulting from processes of subjectification in the housing sector linked to the raising of indebtedness as a main dispositif of capitalism under neoliberal/austerity urbanism agendas. The political economy‐oriented literature on neoliberal/austerity urbanism is bridged with the post‐Marxist approach of Maurizio Lazzarato. Debt is seen as the archetype of social relations, shaping and controlling subjectivities, making the “work on yourself” essential to the reproduction of (indebted) society. However, given the circular nature of power, indebtedness can be generative of new processes of subjectification aimed at subverting the same power relation. In this sense, the paper operationalizes the conceptualization of Foucauldian subjectification recently proposed by Judith Revel, emphasizing how subjectification always results from (1) an action/gesture and (2) a consequent deconstruction of the identity.
Design Aspect of Scraper Blade Assembly for Enhancing Film Heat Transfer Coefficient of Scraped Surface Heat Exchanger
Authors: Juyal Dhruv , Sawhney I.K. , Dodeja A.K. , Minz P.S. 
Published: May 2016

AbstractScraped surface heat exchangers (SSHEs) are known for thermal processing of viscous food products. Heat transfer coefficient is one of the main parameter which influences the performance of processing liquid products in SSHE. The present study was undertaken to determine the film heat transfer coefficient for different blade assembly configurations. Milk was concentrated from 15 to 26% total solids using SSHE. Effect of number of blades (2, 4, 6), weight of blades (1.6, 1.75, 2.0 kg) and rotor speed (150, 175, 200 rpm) was investigated on scraped film heat transfer coefficient (hs) using Response Surface Methodology. The results showed that hs increased linearly with increase in number of blades as well as rotor speed and that it was inversely related to blade weight. The hs varied from 695.3 to 1,415.4 W/m2K. The study was helpful in determining the design factors affecting film heat transfer coefficient of SSHE.Practical ApplicationsIn many of the earlier studies on SSHE, effect of scraper speed, flow rate or the number of blades has been individually studied. Therefore, effort was made to study the combined effect of these parameters. This article gives important insights on the design aspect of rotor blade assembly for thin film SSHE.
A role for loop G in the β1 strand in GABAA receptor activation
Authors: Baptista‐Hon Daniel T. , Krah Alexander , Zachariae Ulrich , Hales Tim G. 
Published: May 2016

Key points
The role of the β1 strand in GABAA receptor function is unclear. It lies anti‐parallel to the β2 strand, which is known to participate in receptor activation.
Molecular dynamics simulation revealed solvent accessible residues within the β1 strand of the GABAA β3 homopentamer that might be amenable to analysis using the substituted Cys accessibility method.
Cys substitutions from Asp43 to Thr47 in the GABAA α1 subunit showed that D43C and T47C reduced the apparent potency of GABA. F45C caused a biphasic GABA concentration–response relationship and increased spontaneous gating.
Cys43 and Cys47 were accessible to 2‐aminoethyl methanethiosulphonate (MTSEA) modification, whereas Cys45 was not. Both GABA and the allosteric agonist propofol reduced MTSEA modification of Cys43 and Cys47.
By contrast, modification of Cys64 in the β2 strand loop D was impeded by GABA but unaffected by propofol.
These data reveal movement of β1 strand loop G residues during agonist activation of the GABAA receptor.
AbstractThe GABAA receptor α subunit β1 strand runs anti‐parallel to the β2 strand, which contains loop D, known to participate in receptor activation and agonist binding. However, a role for the β1 strand has yet to be established. We used molecular dynamics simulation to quantify the solvent accessible surface area (SASA) of β1 strand residues in the GABAA β3 homopentamer structure. Residues in the complementary interface equivalent to those between Asp43 and Thr47 in the α1 subunit have an alternating pattern of high and low SASA consistent with a β strand structure. We investigated the functional role of these β1 strand residues in the α1 subunit by individually replacing them with Cys residues. D43C and T47C substitutions reduced the apparent potency of GABA at α1β2γ2 receptors by 50‐fold and eight‐fold, respectively, whereas the F45C substitution caused a biphasic GABA concentration–response relationship and increased spontaneous gating. Receptors with D43C or T47C substitutions were sensitive to 2‐aminoethyl methanethiosulphonate (MTSEA) modification. However, GABA‐evoked currents mediated by α1(F45C)β2γ2 receptors were unaffected by MTSEA, suggesting that this residue is inaccessible. Both GABA and the allosteric agonist propofol reduced MTSEA modification of α1(D43C)β2γ2 and α1(T47C)β2γ2 receptors, indicating movement of the β1 strand even during allosteric activation. This is in contrast to α1(F64C)β2γ2 receptors, where only GABA, but not propofol, reduced MTSEA modification. These findings provide the first functional evidence for movement of the β1 strand during gating of the receptor and identify residues that are critical for maintaining GABAA receptor function.
Reflections on the new Scottish innovative child protection system
Authors: Agnew Robyn 
Published: May 2016

This paper reflects on my work in Scotland in child protection during 2009 and 2010. It reflects on the Scotland I discovered and The Highlands I worked in. It describes recent innovative political decisions that have informed social work practice across all professions and government departments. It describes the implementation of an essentially simple system, which provides support for every child in need, specific to that need. It is a system that could positively inform the further development of child protection in New Zealand. Given the current plan to seek ways to ‘modernise’ Child Youth and Family (CYF), this paper seeks to encourage a debate on the merits of this path-finding Scottish solution to their political, social, ethnic and professional barriers, which could also produce positive outcomes for children in New Zealand (Tolley, 2015). It describes the overlaying of this approach on top of a professional workforce, despite the silo bureaucracy of service delivery and regardless of professional jealousies protecting individual professions. It describes the responsibilities of all who interact with children and sets certain overlying responsibilities for ‘named’ persons. In this way the responsibilities for the protection of children is moved from the realm of the social work profession, which is overloaded, as it is currently in New Zealand, and applied directly to all professions that interact with children. The Scots have produced a reliable system which strengthens protective mechanisms at the point of service delivery to the child. This in turn develops a reliable and accountable protective society, in which children in need are identified early and supported throughout their contact with different services. The goal is an on-going intervention that can achieve change for the child and allow the child to reach their potential. It is an aspirational system of care, aiming for development of innate potential. I reflect on the system and the safety it provided to myself as a social worker, the relief of ‘sharing’ child protection responsibilities with all other professions and the clarity of roles which defines this system.
First principles calculations of formation energies and elastic constants of inclusions α-Al2O3, MgO and AlN in aluminum alloy
Authors: Liu Yu , Huang Yuanchun , Xiao Zhengbing , Yang Chuge , Reng Xianwei 
Published: May 2016

In this paper, the formation energies and elastic constants of [Formula: see text]-Al2O3, MgO and AlN in both rock salt (cubic) and wurtzite (hexagonal) structures were investigated by first principles calculations. The results show that the formation energy being −17.8, −6.3, −3.06 and −3.46 eV/formula unit for [Formula: see text]-Al2O3, MgO, AlN (rock salt) and AlN (wurtzite). It suggests that in the ground state, [Formula: see text]-Al2O3 is relatively more stable than MgO and AlN. The elastic properties for a polycrystalline in the ground state were calculated with the obtained elastic constants, the elastic properties reveal the rock salt structure AlN is the hardest particles among all the inclusions, and all of these inclusions are classified as brittle materials, which is detrimental to the ductile nature of aluminum matrix. The calculated anisotropy index shows that the AlN (wurtzite) and [Formula: see text]-Al2O3 have a lower degree of anisotropy compared with MgO and AlN (rock salt). The calculated results are in good agreement with the values of experimental and other works.
Matching effective few-nucleon theories to QCD
Authors: Kirscher J. 
Published: May 2016

The emergence of complex macroscopic phenomena from a small set of parameters and microscopic concepts demonstrates the power and beauty of physical theories. A theory which relates the wealth of data and peculiarities found in nuclei to the small number of parameters and symmetries of quantum chromodynamics is by that standard of exceptional beauty. Decade-long research on computational physics and on effective field theories facilitate the assessment of the presumption that quark masses and strong and electromagnetic coupling constants suffice to parametrize the nuclear chart. By presenting the current status of that enterprise, this article touches the methodology of predicting nuclei by simulating the constituting quarks and gluons and the development of effective field theories as appropriate representations of the fundamental theory. While the nuclear spectra and electromagnetic responses analyzed computationally so far with lattice QCD are in close resemblance to those which intrigued experimentalists a century ago, they also test the theoretical understanding which was unavailable to guide the nuclear pioneers but developed since then. This understanding is shown to be deficient in terms of correlations amongst nuclear observables and their sensitivity to fundamental parameters. By reviewing the transition from one effective field theory to another, from QCD to pionful chiral theories to pionless and eventually to cluster theories, we identify some of those deficiencies and conceptual problems awaiting a solution before QCD can be identified as the high-energy theory from which the nuclear landscape emerges.