bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of prion protein function by focal neurite stimulation
Authors: Amin Ladan , Nguyen Xuan T. A. , Rolle Irene Giulia , D'Este Elisa , Giachin Gabriele , Tran Thanh Hoa , Šerbec Vladka Čurin , Cojoc Dan , Legname Giuseppe 
Published: September 2016

ABSTRACT
The cellular prion protein (PrPC), encoded by the PRNP gene, is a ubiquitous glycoprotein, which is highly expressed in the brain. This protein, mainly known for its role in neurodegenerative diseases, is involved in several physiological processes including neurite outgrowth. By using a novel focal stimulation technique, we explored the potential function of PrPC, in its soluble form, as a signaling molecule. Thus, soluble recombinant prion proteins (recPrP) encapsulated in micro-vesicles were released by photolysis near the hippocampal growth cones. Local stimulation of wild-type growth cones with full-length recPrP induced neurite outgrowth and rapid growth cone turning towards the source. This effect was shown to be concentration dependent. Notably, PrPC-knockout growth cones were insensitive to recPrP stimulation, but this property was rescued in PrP-knockout growth cones expressing GFP–PrP. Taken together, our findings indicate that recPrP functions as a signaling molecule, and that its homophilic interaction with membrane-anchored PrPC might promote neurite outgrowth and facilitate growth cone guidance.
Direct interaction between exocyst and Wave complexes promotes cell protrusions and motility
Authors: Biondini Marco , Sadou-Dubourgnoux Amel , Paul-Gilloteaux Perrine , Zago Giulia , Arslanhan Melis D. , Waharte François , Formstecher Etienne , Hertzog Maud , Yu Jinchao , Guerois Raphael , Gautreau Alexis , Scita Giorgio , Camonis Jacques , Parrini Maria Carla 
Published: September 2016

ABSTRACT
Coordination between membrane trafficking and actin polymerization is fundamental in cell migration, but a dynamic view of the underlying molecular mechanisms is still missing. The Rac1 GTPase controls actin polymerization at protrusions by interacting with its effector, the Wave regulatory complex (WRC). The exocyst complex, which functions in polarized exocytosis, has been involved in the regulation of cell motility. Here, we show a physical and functional connection between exocyst and WRC. Purified components of exocyst and WRC directly associate in vitro, and interactions interfaces are identified. The exocyst–WRC interaction is confirmed in cells by co-immunoprecipitation and is shown to occur independently of the Arp2/3 complex. Disruption of the exocyst–WRC interaction leads to impaired migration. By using time-lapse microscopy coupled to image correlation analysis, we visualized the trafficking of the WRC towards the front of the cell in nascent protrusions. The exocyst is necessary for WRC recruitment at the leading edge and for resulting cell edge movements. This direct link between the exocyst and WRC provides a new mechanistic insight into the spatio-temporal regulation of cell migration.
Mature lipid droplets are accessible to ER luminal proteins
Authors: Mishra Shirish , Khaddaj Rasha , Cottier Stéphanie , Stradalova Vendula , Jacob Claire , Schneiter Roger 
Published: September 2016

ABSTRACT
Lipid droplets are found in most organisms where they serve to store energy in the form of neutral lipids. They are formed at the endoplasmic reticulum (ER) membrane where the neutral-lipid-synthesizing enzymes are located. Recent results indicate that lipid droplets remain functionally connected to the ER membrane in yeast and mammalian cells to allow the exchange of both lipids and integral membrane proteins between the two compartments. The precise nature of the interface between the ER membrane and lipid droplets, however, is still ill-defined. Here, we probe the topology of lipid droplet biogenesis by artificially targeting proteins that have high affinity for lipid droplets to inside the luminal compartment of the ER. Unexpectedly, these proteins still localize to lipid droplets in both yeast and mammalian cells, indicating that lipid droplets are accessible from within the ER lumen. These data are consistent with a model in which lipid droplets form a specialized domain in the ER membrane that is accessible from both the cytosolic and the ER luminal side.
Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM–Orai interactions in Drosophila neurons
Authors: Chakraborty Sumita , Deb Bipan K. , Chorna Tetyana , Konieczny Vera , Taylor Colin W. , Hasan Gaiti 
Published: September 2016

ABSTRACT
Store-operated Ca2+ entry (SOCE) occurs when loss of Ca2+ from the endoplasmic reticulum (ER) stimulates the Ca2+ sensor, STIM, to cluster and activate the plasma membrane Ca2+ channel Orai (encoded by Olf186-F in flies). Inositol 1,4,5-trisphosphate receptors (IP3Rs, which are encoded by a single gene in flies) are assumed to regulate SOCE solely by mediating ER Ca2+ release. We show that in Drosophila neurons, mutant IP3R attenuates SOCE evoked by depleting Ca2+ stores with thapsigargin. In normal neurons, store depletion caused STIM and the IP3R to accumulate near the plasma membrane, association of STIM with Orai, clustering of STIM and Orai at ER–plasma-membrane junctions and activation of SOCE. These responses were attenuated in neurons with mutant IP3Rs and were rescued by overexpression of STIM with Orai. We conclude that, after depletion of Ca2+ stores in Drosophila, translocation of the IP3R to ER–plasma-membrane junctions facilitates the coupling of STIM to Orai that leads to activation of SOCE.
Intraarticular corticosteroid injection in adhesive capsulitis: are two doses better than a single dose? a randomized prospective study
Authors: Biradar Ramanagouda , Nandi Santosh S. , Patel Amit , Pillai Arravind 
Published: September 2016

<p class="abstract"><strong>Background:</strong> The present study aimed to compare the effectiveness of a single dose and two doses of intraarticular corticosteroids injections followed by home exercise programme in patients with adhesive capsulitis.</p><p class="abstract"><strong>Methods:</strong> The study was done over two years. Eighty four patients with adhesive capsulitis were enrolled in the study. The patients were randomly assigned to two groups: In group I 38 patients were given a single dose of intraarticular corticosteroid injection (1 mL, 40 mg methylprednisolone acetate) followed by a twelve-week home exercise programme. In group II 46 patients were given two doses of intraarticular corticosteroid injection (1 mL, 40 mg methylprednisolone acetate) at first and third week followed by home exercise programme. All the patients were assed for functional out come at six and twelve weeks using Shoulder pain and disability index (SPADI) and Constant Moore’s shoulder score (CMS).<strong></strong></p><p class="abstract"><strong>Results:</strong> Both groups showed considerable improvement from the baseline, but no significant differences were found between the two groups at twelve weeks. Mean changes in range of motion and shoulder pain and disability index–pain score were statistically no different between the two groups at the twelve weeks.</p><p><strong>Conclusions:</strong> Intraarticular corticosteroids have the additive effect of providing rapid pain relief when combined with home exercise program in adhesive capsulitis. No significant differences in outcome were found in patients treated with a single or two doses of corticosteroid injection.</p>
VAS score assessment for outcome of posterior lumbar inter body fusion in cases of lumbar canal stenosis
Authors: Murthy Harish , Reddy T V S 
Published: September 2016

<p class="abstract"><strong>Background:</strong> One of the major causes for disability in adult working population is degenerative lumbosacral spine disorders are fairly common in middle aged and elderly population. Lumbar canal stenosis remains one of the most frequently encountered clinically important degenerative spinal disorders requiring operative treatment in the aging population. The objective of the present study is to assess the outcome of posterior lumbar inter body fusion in cases of lumbar canal stenosis</p><p class="abstract"><strong>Methods:</strong> The present study, 30 cases of lumbar canal stenosis, who were treated operatively with decompression and posterior lumbar inter body fusion, which was carried out over a period of 6 months in a tertiary care center were included. 16 women and 14 men were included in the study.<strong></strong></p><p><strong>Results:</strong> Most patients were in the age group of 41-50 years (36.7%) followed by 51-60 years (33.3%). In this study it was found that there was significant improvement in VAS score for back pain and leg pain over the 6 month follow-up. There is significant difference between mean improvement in VAS score with respect to number of levels involved for leg pain (p =0.01). There is no statistical significance difference between number of levels involved and improvement in back pain (p =0.66).</p><strong>Conclusions:</strong>VAS score showed posterior lumbar interbody fusion with interbody cage and local graft with posterior instrumentation gave significantly improved clinical and functional outcome by causing significant reduction in pain and patient disability.<p> </p>
Outcome of tibialis posterior tendon transfer to middle cuneiform by interference screw technique for foot drop
Authors: Babu BLS Kumar , Ravindran Biju 
Published: September 2016

<p class="abstract"><strong>Background:</strong> Foot drop is a common problem which compromises the quality of life and impairs activities of daily living. Walking becomes difficult with foot drop because the foot quickly hits the obstacles, or the knee has to be more flexed than usual when going up stairs. Transfer of the tibialis posterior tendon restores active dorsiflexion of the foot, while tenodesis prevents the foot from dropping. To this purpose, we explored the results of this technique in a small cohort of foot drop patients.</p><p class="abstract"><strong>Methods:</strong> Twenty-five patients with foot drop due to various causes such as tibial condyle fracture, fracture dislocation of the hip, post hip surgeries, late compartment syndrome and due to spinal aetiology by lumbar canal stenosis, post spinal surgeries due to discectomy and spondylolisthesis were included. Patients with post-traumatic arthritis of ankle, metabolic causes, poliomyelitis, sensory and motor neuropathy were excluded. Patients underwent anterior transposition of the tibialis posterior tendon (PTT) to the middle cuneiform through the interosseous membrane using tenodesis screw technique. The outcomes measured 2, 6 12 and 24 weeks postoperatively were a power of dorsiflexion, use of footwear to assess the dorsiflexion of the ankle in swing phase and at heel strike, hindfoot valgus patient satisfaction and activities of daily living.<strong></strong></p><p class="abstract"><strong>Results:</strong> The average age of the patients was 46±7.4 years. 18/25 (72%) had the 4+/5 power of dorsiflexion, 7/25 (28%) had 4-/5 power. 23/25 (92%) of patients use of footwear of certain types, suggesting that these patients improved from a high-stepping gait to a heel-toe gait. 18/25 (72%) patients were very satisfied, and 4/25 (16%) were happy, 3/25 (12%) patients were not satisfied. We found that 14/25 (56%) were having regular daily activity, and rational recreation and none of our patient had a severe limitation on functional assessment. All the 25 patients in our cohort exhibited dorsiflexion of the ankle in swing phase by 12 weeks post operatively.3/25 (12%) had hindfoot valgus postoperatively. None of our patients had wound complication at the insertion site. </p><p><strong>Conclusions:</strong> Tenodesis screw technique for insertion site fixation showed 72% result in restoring ankle function with a similar patient satisfaction and minimal complications.</p>
Cannulated cancellous screws fixation in intracapsular fracture neck femur: a study with an emphasis on result of osteosynthesis
Authors: Vijay Varun , Srivastava Naveen 
Published: September 2016

<p class="abstract"><strong>Background:</strong> Fracture neck of femur has always presented a great challenge to the orthopaedic surgeons. It is rightly called as “unsolved fracture” as far as treatment and results are concerned. Results generally depend upon time period elapsed from fracture to surgery, adequacy of reduction and fixation. Fixation with cannulated cancellous screw is usually adequate for femoral neck fractures. The aim of the study was to analyse the results of treatment of fracture neck of femur with cannulated cancellous screw fixation and to compare the results with others in the literature using the same modality. </p><p class="abstract"><strong>Methods:</strong> 25 patients with intra capsular neck of femur fracture were followed for a period of two years post-surgery and their functional outcome was assessed based upon harris hip scoring system.<strong></strong></p><p class="abstract"><strong>Results:</strong> According to harris hip scoring system, we had excellent results in 72% cases, good in 16% cases, fair in 8% and poor in 4%. One patient went into non-union and two developed avascular necrosis of femoral head.</p><p><strong>Conclusions:</strong> Management of intracapsular fracture neck of femur with cannulated cancellous screw fixation is a very good method of treatment being a surgically easy procedure. Use of multiple cannulated cancellous screw have a compression effect at the fracture site. It also avoids re displacement and rotation.</p>
Compartment pressure changes after closed fractures of tibia
Authors: Pillai Manju G. 
Published: September 2016

<p><strong>Background: </strong>Compartment syndrome is a potentially devastating situation. Raised intracompartmental pressure has been implicated as the primary pathogenic factor in compartment syndrome. The purpose of the study was early detection of compartment syndrome and corroborating the findings with other physical signs and symptoms, to prevent the onset of ischaemia and subsequent tissue changes that lead to crippling deformities.</p><p><strong>Methods:</strong> The present study was conducted in the Department of Orthopaedics, Pushpagiri medical college hospital, Thiruvalla over a period of 12 months. Closed fractures of tibia admitted to the casualty unit within 36 hours of injury were selected for the study. A total of 24 patients were included with the majority in the age group of 31– 45 years. Whitesides technique was used to measure the compartment pressure. A differential pressure of less than 30 mm Hg was taken as the criterion for diagnosis of compartment syndrome. </p><p><strong>Results:</strong> The present study included 24 patients with affected 25 limbs.15 out of 25 limbs were with lower third fractures (60%) followed by upper third 6 (24%) and middle third 4 (16%). Out of 25 limbs 20 cases (80%) had associated fracture of fibula and 5 (20%) were not associated. In this study, out of 32% cases with increased compartment pressure, one case with upper third fracture (above 45 mm Hg) and one of the case with middle third fracture (20-30 mm Hg) with associated fibula had underwent immediate fasciotomy.</p><strong>Conclusion:</strong> Compartment pressure measurement is a very good index for predicting and preventing compartment syndrome. Fasciotomy to fully decompress all involved compartments is the definitive treatment for compartment syndrome in the great majority of cases. Delays in performing fasciotomy increase morbidity.
"Usted, Joseph Conrad, me ha robado": de Nostromo a Historia Secreta de Costaguana
Authors: Gaitán Bayona Jorge Ladino 
Published: September 2016

Este artículo analiza Historia secreta de Costaguana (2007), de Juan Gabriel Vásquez. La novela entra en diálogo irreverente con Nostromo (1904), de Joseph Conrad, y con la historia de Colombia del siglo XIX e inicios del siglo XX. Narra con intensidad la Guerra de los Mil Días y la pérdida de Panamá. La historia y la ficción se entrelazan para cuestionar el pasado de Colombia. Además, se relatan los procesos que rodean la creación literaria. El artículo toma como línea teórica la metaficción historiográfica, a partir de Linda Hutcheon. Del mismo modo, se recurrirá a conceptos de Fernando Ainsa sobre la reescritura del pasado.Palabras clave: Novela, Conrad, Colombia, Panamá, metaficción historiográfica.
El ruido de las cosas al caer: La conciencia histórica como respuesta a la estética de la narco novela en Colombia
Authors: Fernández Luna Paola 
Published: September 2016

En este artículo propongo que la estética de la narco novela en Colombia ha sido un fenómeno que ha tenido gran acogida en las ventas de consumo editorial y en la recepción de los lectores. Escritores como Jorge Franco en el caso de la narrativa y, en las crónicas Gustavo Bolívar, entre otros, calcan a través de sus propuestas figuras estereotipadas de la realidad. Así mismo, considero que esta estética ha explotado la figura del narco, la violencia en las ciudades y el lenguaje propio de esta representación del mundo. Elementos que hacen merecer la atención en el público para el cual la historia narrada es más interesante en cuanto más efectista. Por el contrario, propuestas como la de Juan Gabriel Vásquez se consolidan dentro del campo de la novela colombiana como un distanciamiento a la tendencia del presentismo propia de la narco novela, cuyo reflejo de la realidad elimina la distancia estética y narrativa por medio de la cual hace sentir al lector complacencia porque éste no sale de sus marcos de cotidianidad.Palabras clave: conciencia histórica, narco estética, novela en Colombia, distanciamiento crítico.
Espacios imaginarios en la poesía colombiana: Álvaro Mutis, Eduardo Cote Lamus, Aurelio Arturo y Carlos Obregón
Authors: Romero Guzmán Nelson 
Published: September 2016

El presente ensayo, apoyado en el enfoque metodológico de la hermenéutica simbólica, ubica la obra de los poetas Álvaro Mutis, Eduardo Cote Lamus, Aurelio Arturo y Carlos Obregón como predecesoras de la poesía moderna en Colombia al crear espacios imaginarios a partir de espacios afectivos o vividos por la experiencia. La fuerte adherencia del lenguaje a expresarse a través del símbolo, permite que las imágenes poéticas se tornen oscuras y ambiguas a favor del significado y el sentido, lo cual hace mucho más particular y atractivo para el lector de hoy sus legados estéticos. En estos autores la poesía, a partir de un espacio afectivo recreado, funda un espacio imaginario. Palabras claves: Poesía colombiana, espacio imaginario, espacio afectivo, mito, mística, hermenéutica, exilio.
Llama, lenguaje, ceniza: estaciones críticas del cuerpo, la literatura, el erotismo y la visualidad
Authors: Garrandés Alberto 
Published: September 2016

Este ensayo, presentado como conferencia magistral del I Congreso Internacional y XXVII Nacional de Lingüística Semiótica y Literatura, es un percurso por la literatura como una experiencia de lenguaje que surge del cuerpo. En estas páginas el autor examina su relación con el lenguaje y los modos de investigación, creación y revelación usados en la construcción de sus obras, todas ellas ficciones agenéricas, performáticas e instalativas.Palabras clave: cuerpo, erotismo, literatura, visualidad.Llama, lenguaje, ceniza: estaciones críticas del cuerpo, la literatura, el erotismo y la visualidad
Del Rhin al Suárez o el viaje de Lengerke por los caminos de la Civilización y la "Barbarie"
Authors: Franco Álvaro Neil 
Published: September 2016

El propósito del presente ensayo es abordar la novela "La otra raya del tigre” de Pedro Gómez Valderrama, desde una perspectiva que enfatiza la crisis de la modernidad y la literatura de viajes característica del siglo XIX. De tal manera, que conceptos como modernización económica, ciudad, liberalismo, colonización, feudalismo y civilización, junto con la visión del crítico moderno Néstor García Canclini y la percepción que Carmen Elisa Acosta tiene sobre los viajeros decimonónicos, son de vital importancia para comprender el itinerario del presente texto por la naturaleza exótica de Santander, por los caminos amarillos salidos de las entrañas de Van Gogh, por la fatalidad de las mujeres con alma de escorpión, por la hibridación de las culturas, especialmente la alemana y la santandereana, y por el lenguaje que destaca la tradición oral de los llamados pingos.
Erotismo Femenino en (Des) encuentros (des) esperados de Andrea Maturana
Authors: Pizarro Lilian Barraza 
Published: September 2016

La literatura erótica en Chile desde una autoría femenina se instala tímidamente en la década de los noventa con la obra: (Des) encuentros (des) esperados de la escritora chilena Andrea Maturana. Este artículo pretende demostrar que en esta obra literaria se revierte el prototipo sexual asignado a la mujer hasta el momento. La investigación procura indagar en el comportamiento de los personajes femeninos en el ámbito erótico, identificar una visión de mundo a través de esas acciones, analizar las voces narrativase ilustrar el panorama histórico social de la década, el cual tiene una alta incidencia en la creación de los personajes, debido a los cambios que se advierten con la Transición.Palabras clave: literatura erótica, Chile, prototipo sexual, mujer, Transición.
Escribir para resistir la Soledad: Nostalgia, confesión y amor en cartas a Clara de Juan Rulfo
Authors: Chede Carlos Julio Ayram 
Published: September 2016

El presente artículo indaga en el conjunto de cartas que el escritor mexicano Juan Rulfo le escribe a Clara Aparicio entre los años 1944 y 1950 las cuales no solo representan un hecho poético en sí mismo, sino que develan al menos tres estados que se conjugan y coexisten en el plano de la expresión de cada misiva: la nostalgia, la confesión y el amor. Por tanto, cada una de las cartas que escribe Rulfo le permite asegurar un lugar en el mundo que empieza a habitar desde la lejanía de su hogar y que comparte de manera permanente con Clara. Juan Rulfo se sustrae de la lógica de la cotidianidad para evaporar la rutinización de su vida en la escritura, podría afirmarse, que como San Juan de la Cruz o Gustavo Adolfo Bécquer, Rulfo se evade en la escritura como oportunidad para albergar un mundo incondicional donde existe él y su corazón, donde existe el sujeto que ama. Palabras clave: Soledad, nostalgia, confesión, escritura, distancia, amor, Juan Rulfo, Clara Aparicio.
Reflexiones sobre la identidad en Macunaíma
Authors: Velásquez Gesica Giohanna Espejo 
Published: September 2016

Este artículo de reflexión es una aproximación a la problemática de identidad existente dentro de la novela Macunaíma de Mario de Andrade. Abordaré dicha temática a partir de los planteamientos de Fernando Ortiz y Leopoldo Zea; planteamientos que se configuran en tres personajes que dentro de la obra cumplen un papel primordial para comprender la propuesta del escritor brasilero. Estos son: Macunaíma, Venceslao Pietro Pietra y las Icamiabas. De este modo, analizo diferentes fragmentos de la novela con el fin de evidenciar ese afán por reconocer y reafirmar la identidad del hombre brasilero.Palabras clave: Identidad, transculturación, pasado indígena, cultura y europeo.
Deux Visions Pataphysiques. Recueil Litchis de Madagascar
Authors: Pardo Segura Martha 
Published: September 2016

donnée par ce dramaturge est “La pataphysique est la science des solutions imaginaires”. Le mot provient de l’oeuvre Gestes et opinions du Docteur Faustroll, pataphysicien (écrit en 1897-1988, publié posthumément en 1911) de l’écrivain Alfred Jarry. (Laval 1873-Paris 1907). Ce nom désigne aussi le mouvement littéraire et culturel français de la seconde moitié du XXème siècle, lié au surréalisme.** Traduction en français par le professeur Martha Pardo Segura. UPTC-Tunja