bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting DLK1 improves survival in patient-derived xenograft models of myeloid leukemia associated with Down syndrome
Authors: Barwe Sonali P. , Tavenner Meredith , van Berkel Patrick , Sebastian Aimy , Ries Rhonda E , Meshinchi Soheil , Hitzler Johann , Pikman Yana , Kolb E. Anders , Gopalakrishnapillai Anilkumar 
Published: September 2024

AbstractMyeloid leukemia associated with Down syndrome (ML-DS) is a rare pediatric cancer. Current standard-of-care includes cytotoxic chemotherapy. 10-20% of patients develop relapsed leukemia or have refractory disease. These patients have a 3-year event-free survival less than 21%. Novel therapy options are needed to improve survival in these patients with relapsed or refractory disease. Our transcriptome analysis to identify transmembrane proteins that are overexpressed in ML-DS compared to normal bone marrow specimens revealed DLK1 as one of the topmost modulated targets. We show that DLK1 knockout in ML-DS cells significantly reduced proliferationin vitroand delayed engraftmentin vivo. Furthermore, targeting DLK1 via antibody drug conjugates significantly prolonged survival compared to control isotype antibody drug conjugate in patient-derived xenograft models of refractory ML-DS. Taken together, targeting DLK1 may be a novel treatment option for refractory ML-DS patients.Key PointsWe identified DLK1 as a novel target differentially expressed in myeloid leukemia associated with Down syndrome (ML-DS).DLK1-targeted antibody drug conjugate was effective in curbing leukemia progression in patient-derived xenograft models of refractory ML-DS
Freeze-dried porous collagen scaffolds for the repair of volumetric muscle loss injuries
Authors: Basurto Ivan M. , Boudreau Ryann D. , Bandara Geshani C. , Muhammad Samir A. , Christ George J. , Caliari Steven R. 
Published: September 2024

AbstractVolumetric muscle loss (VML) injuries are characterized by the traumatic loss of skeletal muscle resulting in permanent damage to both tissue architecture and electrical excitability. To address this challenge, we previously developed a 3D aligned collagen-glycosaminoglycan (CG) scaffold platform that supportedin vitromyotube alignment and maturation. In this work, we assessed the ability of CG scaffolds to facilitate functional muscle recovery in a rat tibialis anterior (TA) model of VML. Functional muscle recovery was assessed following implantation of either non-conductive CG or electrically conductive CG-polypyrrole (PPy) scaffolds at 4, 8, and 12 weeks post-injury byin vivoelectrical stimulation of the peroneal nerve. After 12 weeks, scaffold-treated muscles produced maximum isometric torque that was significantly greater than non-treated tissues. Histological analysis further supported these reparative outcomes with evidence of regenerating muscle fibers at the material-tissue interface in scaffold-treated tissues that was not observed in non-repaired muscles. Scaffold-treated muscles possessed higher numbers of M1 and M2 macrophages at the injury while conductive CG-PPy scaffold-treated muscles showed significantly higher levels of neovascularization as indicated by the presence of pericytes and endothelial cells, suggesting a persistent wound repair response not observed in non-treated tissues. Finally, only tissues treated with non-conductive CG scaffolds displayed neurofilament staining similar to native muscle, further corroborating isometric contraction data. Together, these findings show that CG scaffolds can facilitate improved skeletal muscle function and endogenous cellular repair, highlighting their potential use as therapeutics for VML injuries.
Task-voting for schizophrenia spectrum disorders prediction using machine learning across linguistic feature domains
Authors: He Rui , de la Foz Víctor Ortiz-García , Cacho Luis Manuel Fernández , Homan Philipp , Sommer Iris , Ayesa-Arriola Rosa , Hinzen Wolfram 
Published: September 2024

AbstractBackground and HypothesisIdentifying schizophrenia spectrum disorders (SSD) from spontaneous speech features is a key focus in computational psychiatry today.Study DesignWe present a task-voting procedure using different speech-elicitation tasks to predict SSD in Spanish, followed by ablation studies highlighting the roles of specific tasks and feature domains. Speech from five tasks was recorded from 92 subjects (49 with SSD and 41 controls). A total of 319 features were automatically extracted, from which 24 were pre-selected based on between-feature correlations and ANOVA F-values, covering acoustic-prosody, morphosyntax, and semantic similarity metrics.Study ResultsExtraTrees-based classification using these features yielded an accuracy of 0.840 on hold-out data. Ablating picture descriptions impaired performance most, followed by story reading, retelling, and free speech. Removing morphosyntactic measures impaired performance most, followed by acoustic and semantic measures. Mixed-effect models suggested significant group differences on all 24 features. In SSD, speech patterns were slower and more variable temporally, while variations in pitch, amplitude, and sound intensity decreased. Semantic similarity between speech and prompts decreased, while minimal distances from embedding centroids to each word increased, and word-to-word similarity arrays became more predictable, all replicating patterns documented in other languages. Morphosyntactically, SSD patients used more first-person pronouns together with less third-person pronouns, and more punctuations and negations. Semantic metrics correlated with a range of positive symptoms, and multiple acoustic-prosodic features with negative symptoms.ConclusionsThis study highlights the importance of combining different speech tasks and features for SSD detection, and validates previously found patterns in psychosis for Spanish.
Pain distribution can be determined by classical conditioning
Authors: Nastaj Jakub , Skalski Jacek , Nowak Daria , Kruszyna Natalia , Bąbel Przemysław , Szikszay Tibor , Luedtke Kerstin , Gnat Rafał , Adamczyk Wacław M. 
Published: September 2024

ABSTRACTChronic widespread pain (CWP) - as many other clinical presentations - manifests in ongoing pain without identifiable structural cause, with pain that spreads over multiple body areas. The development and maintenance of symptoms may involve learning mechanisms. Ninety-four healthy volunteers participated in this study and were randomly distributed to four groups. In the classical conditioning combined with verbal suggestion group, US-(small pain distribution) and US+ (large pain distribution) were paired with visual stimuli (CS+ and CS-) and participants were told about this association. In the verbal suggestion group, the conditioning was not performed, whereas in classical conditioning only group, learning was not combined with suggestion. In the control group, conditioning and suggestion did not take place. Ratings of perceived pain distribution (PD) were collected after each trial and ratings of pain intensity after each block of trials. During the testing phase, participants were exposed to electrocutaneous stimuli corresponding to only the small (US-) pain distribution. Results showed significant differences between CS+ and CS-pain distribution ratings across the experimental groups: conditioning + verbal suggestion (p<0.01), conditioning only group (p<0.05) and verbal suggestion only group (p<0.05), but not in the control group (p>0.05). Furthermore, significant differences in the perceived pain distribution were found between the control group and all experimental groups. This result supports our main hypothesis that the perceived pain distribution can be influenced by classical conditioning as well as verbal suggestion, although the effect is stronger when both are combined.
Distal super-enhancer drives aberrantCXCL13expression in Cancer cells driving growth and p53 dysregulation via CXCR5-CXCL13 axis
Authors: Gothwal Santosh Kumar , Mattila Pieta K. , Barlow Jacqueline H 
Published: September 2024

AbstractThe CXCL13 chemokine plays a crucial role in guiding B cell migration to the light zones (LZs) during the germinal center (GC) reaction. WhileCXCL13expression is absent in most cell types, aberrant amplification of the CXCR5-CXCL13 signaling is observed in various cancers, including germinal center-derived B-lymphomas (GCDBL), colorectal adenocarcinoma (COAD), and liver hepatocellular carcinoma (LIHC). However, the molecular mechanisms underlying abnormalCXCL13transcription in cancer cells and its functional consequences remain elusive. We identify DNA-CpG methylation binding protein 1 (MBD1) as a suppressor ofCXCL13expression. Chromosomal conformation capture (3C) analysis reveals a distal super-enhancer located nearCCNG2that interacts with theCXCL13promoter in GCDBL, suggesting that enhancer-hijacking drives the aberrant expression. Our functional validation demonstrates that CXCR5-CXCL13 signaling suppresses p53 and its target genes in GCDBLs, COAD, and LIHC. Notably, CXCL13 in the GCDBL cell line Raji disrupts CXCR5-mediated migration, a mechanism essential for (light zone) LZ-entry and affinity maturation of GC B cells. These findings highlight the dual role of the CXCR5-CXCL13 axis in immune response and cancer proliferation.Key PointsSuper-enhancer nearCCNG2region interacts withCXCL13-TSSdriving CXCL13 in cancers.Aberrant CXCL13 prevents CXCR5-mediated migration of B-lymphomas and promotes growth and p53 dysregulation in CXCR5+ cellsCXCR5-CXCL13 axis impairs p53 target gene expression and promotes tumor growthAbstract FigureHighlightsAberrantCXCL13expression in hematological and solid cancersChemotherapeutic treatment of cancer cells promotesCXCL13andCXCR5expressionDistal super-enhancer onCCNG2interacts withCXCL13promoterCXCL13 expression in B-lymphomas prevents CXCR5-dependent migrationCXCR5-CXCL13 axis encounters p53 function in hematological and solid cancer cells
Vision is not olfaction: impact on the insect Mushroom Bodies connectivity
Authors: Le Moël Florent , Wystrach Antoine 
Published: September 2024

AbstractThe Mushroom Bodies, a prominent and evolutionary conserved structure of the insect brain, are known to be the support of olfactory memory. There is now evidence that this structure is also required for visual learning, but the hypotheses about how the view memories are encoded are still largely based on what is known of the encoding of olfactory information. The different processing steps happening along the several relays before the Mushroom Bodies is still unclear, and how the visual memories actually may allow navigation is entirely unknown. Existing models of visual learning in the Mushroom Bodies quickly fall short when used in a navigational context. We discuss how the visual world differs from the olfactory world and what processing steps are likely needed in order to form memories useful for navigation, and demonstrate it using a computational model of the Mushroom Bodies embedded in an agent moving in through a virtual 3D world.
Pursuing reconsolidation in ethanol-CPP: memory reactivation in different conditions did not trigger destabilization
Authors: Zacouteguy Boos Flávia , Déo Sorigotto Beatriz , Favoretto Cristiane Aparecida , Cruz Fabio Cardoso , Quadros Isabel Marian Hartmann de 
Published: September 2024

ABSTRACTConsolidated memories can return to a labile state during retrieval, through destabilization, and must be reconsolidated to persist. Associative memories of contextual cues paired with hedonic effects of drugs of abuse exert a pivotal role in maintaining maladaptive behaviors in addiction. Thus, impairment of reconsolidation of drug-associated memories may provide a potential strategy to reduce drug-seeking and relapse in addiction. It is critical to understand the conditions under which a consolidated memory becomes labile and may undergo reconsolidation. After inducing ethanol Conditioned Place Preference (CPP; 2 g/kg ethanol, i.p.) in male mice, we examined different parameters during the reactivation session that could turn memory susceptible to disruption by systemic injection of the protein synthesis inhibitor cycloheximide (CHX, 100 mg/kg, i.p.). Reactivation with free access to the apparatus (similarly to a Test session, for 10, 5, or 3 min) or Reactivation sessions restricted to ethanol-paired compartment with no ethanol (for 10 or 5 min), or with the administration of a low dose of ethanol (5 min session), failed to reduced ethanol-preference after CHX administration. These findings suggest that boundary conditions constraint memory in ethanol-CPP to undergo reconsolidation.
Connectivity between supplementary motor complex and primary motor cortex: a dual-coil paired-pulse TMS study
Authors: Kim Hakjoo , Lei Yuming , Bao Shancheng , Huynh Angelina T. , Buchanan John J. , Bernard Jessica A. , Brown Joshua C. , Wright David L. 
Published: September 2024

SUMMARYIn recent years, dual-coil paired-pulse transcranial magnetic stimulation (ppTMS) has garnered interest for its potential in elucidating neural circuit dynamics. In this study, the dual-coil ppTMS was utilized to assess the effective connectivity between the supplementary motor complex (SMC) and the primary motor cortex (M1) in humans. A robust facilitatory connection between the SMC and M1 was observed, manifested as a 19% increase in mean peak-to-peak motor-evoked potentials following preconditioning of SMC 7 ms prior to M1 stimulation. Importantly, the facilitatory influence of SMC only occurred when the preconditioning stimulation was administered 4 cm anterior to Cz but not when applied at 5-cm, 6-cm, or 7-cm distance. While previous work has focused on demonstrating important temporal dynamics for SMC-M1 plasticity, the present findings highlight a critical contribution of spatial specificity for modulation of SMC-M1 circuitry.Graphical abstractHighlightsThe conditioned stimulus induced larger MEPs compared to the unconditioned stimulusThe connectivity between SMC and M1 diminished when the SMC coil was moved forwardFindings highlight the importance of spatial specificity for modulation of SMC-M1 circuitry
Developing an evaluation framework for public health environmental surveillance: Protocol for an international, multidisciplinary e-Delphi study
Authors: Manuel Douglas G. , Bennett Carol , Brown Emma , Buckeridge David L. , Freedhoff Yoni , Funnell Sarah , Ishtiaq Farah , Wade Matthew J. , Moher David 
Published: September 2024

AbstractIntroductionPublic health environmental surveillance has evolved, especially during the coronavirus disease pandemic, with wastewater-based surveillance being a prominent example. As surveillance methods diversify and expand, it is essential to have a robust evaluation of surveillance systems. This electronic Delphi study will propose an evaluation framework for public health environmental surveillance, informed by the expanding practice of wastewater-based surveillance during the pandemic.MethodsThe Public Health Environmental Surveillance Evaluation Framework (PHES-EF) will be developed in five steps. In Step 1, a multinational and multidisciplinary executive group will be formed to guide the framework development process. In Step 2, candidate items for Round 1 of the electronic Delphi will be generated by conducting relevant scoping reviews and consultation with the study executive group. In Step 3, an international electronic Delphi will be conducted over two rounds to develop consensus on items for the framework. In Step 4, the executive group will reconvene to finalize the evaluation framework, discuss standout items, and determine the dissemination strategies. Lastly, Step 5 will focus on disseminating the evaluation framework to all parties involved with or affected by wastewater-based surveillance using traditional and public-oriented methods.DiscussionThe electronic Delphi study will provide multidisciplinary and multinational consensus for the evaluation framework for public health environmental surveillance by providing a set of minimum criteria required for their evaluation. The evaluation framework is intended to support the sustainability of environmental surveillance and improve its implementation, reliability, credibility, and value.
Genetic diversity and differentiation of a mycoheterotrophic orchid (Cymbidium macrorhizon)under urbanization
Authors: Ishii Naohiro I. , Yamamoto Satoshi , Iwachido Yuki , Uchida Kei 
Published: September 2024

AbstractUrbanization exerts substantial pressures on genetic diversity of plant species. However, depending on species-specific life history, the direction/magnitude of urbanization impacts can vary. To elucidate relationships between life history and urbanization effects, there are needs to accumulate the knowledge on genetic diversity/differentiation along urban-rural gradients for species with unique traits. We examined these facets based on genome-wide single nucleotide polymorphisms of a mycoheterotrophic and vegetative-dormant orchid,Cymbidium macrorhizon, for eleven populations in remnant forests along an urban gradient within the Tokyo metropolitan area, Japan. The reduced inbreeding coefficient and increased genetic differentiation were observed with increased proportion of surrounding urban land-use 50 years ago rather than in recent years. This pattern might reflect lowest heterozygosity under intensive urbanization due to population bottleneck and genetic drift due to habitat shrinkage and fragmentation. The significant impacts of past landscape on the indices might indicate time lags of genetic erosion, namely intra-specific extinction debt, due to longevity and dormancy. Therefore, 30% increase of urban land-use since 1970s has not yet affected genetic erosion, resulting in its progression in the future. We emphasize the importance not only to assess genetic diversity but also to connect the assessments with life history and spatiotemporal urbanization impacts.
Beats of the Brain: Mapping Neural Activation During Candombe Engagement
Authors: Agarwal Netra 
Published: September 2024

AbstractThis research delves into the complex connection between traditional music in Uruguay, specifically candombe, and its cognitive advantages. Relying on real-life studies, we explore the impact of involvement with candombe music on diverse cognitive abilities such as cognitive flexibility, emotional regulation, and social cognition. Our results show that playing music, especially drumming, activates certain brain pathways related to cognitive function and emotional regulation.The sense of community and distinct rhythmic patterns found in candombe not only boost individual cognitive skills but also foster social bonding, ultimately leading to better mental well-being and strength. This study highlights how cultural heritage influences cognitive functions, showing that candombe is crucial for mental well-being in addition to being a significant cultural symbol.In summary, our research supports incorporating traditional Uruguayan music in educational and therapeutic environments, offering a new framework for cognitive and emotional growth. This research focuses on how Uruguay’s diverse musical traditions can be used to enhance cognitive abilities and mental health, offering possibilities for application in different cultural settings globally.
A reliance on human habitats is key to the success of an introduced predatory reptile
Authors: Major Tom , Jeffrey Lauren , Russel Guillem Limia , Bracegirdle Rebecca , Gandini Antonio , Morgan Rhys , Marshall Benjamin Michael , Mulley John F. , Wüster Wolfgang 
Published: September 2024

AbstractUnderstanding the success of animals in novel environments is increasingly important as human-mediated introductions continue to move species far beyond their natural ranges. Alongside these introductions, inhabited and agricultural areas are spreading, and correspondingly most animal introductions occur in populated areas. Commensal species which can live alongside humans by making use of specific conditions, structures, or prey, have a significant advantage. Introduced mammal species often use anthropogenic features in their environment and demonstrate a higher tolerance of human disturbance, but their importance remains understudied in ectotherms. The Aesculapian snake (Zamenis longissimus) is an ectotherm which has been introduced beyond the northern extremities of its natural range. To understand their persistence, we radio-tracked snakes daily over two active seasons, including high-frequency tracking of a subset of males. We investigated snake home range size using Autocorrelated Kernel Density Estimators (AKDE). Using AKDE-weighted Habitat Selection Functions we identified preferences for habitat features in a mosaic of habitats, and we used Integrated Step Selection Functions to further explore how these features influence movement. We revealed a particular preference for buildings in male snakes, while females preferred woodland. We demonstrate that the success of this ectothermic predator is likely tied to a willingness to use human features of the landscape.
Longitudinal trajectories of psychological resilience and cognitive impairment among older adults: evidence from a national cohort study
Authors: Wang Peicheng , Li Ruihua , Chen Yanhua 
Published: September 2024

AbstractBackgroundThe relationship between resilience trajectories and cognitive health is not well understood. This study aimed to identify subgroups of psychological resilience trajectories in a national sample of older adults and to examine the association with cognitive impairment over time.MethodsThis study used data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) from 2008 to 2018, and 2,788 respondents were included in this prospective analysis. Using a group□based trajectory modeling approach, we identified resilience trajectory groups within a 6-year period. Cox proportional hazards models were used to assess the relationship between the resilience trajectory groups and cognitive impairment.ResultsThree distinct trajectories of psychological resilience, including decreasing resilience group (n=131,7.1%), persistent middle resilience group (n=1,808, 58.1%), and persistent high resilience group (n=849, 34.8%). During the 6-year follow-up, compared to those with persistent high resilience, participants with persistent middle resilience (HR=1.43, 95% CI=1.14-1.79) and decreasing resilience (HR=2.46, 95% CI=1.76-3.43) remained consistent associated with a higher risk of cognitive impairment. The associations between resilience trajectories and cognitive impairment varied by lifestyle and health conditions.ConclusionsPsychological resilience is a relatively stable trait among older adults in China, with most individuals maintaining a persistently high or middle level of resilience throughout the follow-up period; however, declining psychological resilience was significantly associated with the risk of cognitive impairment. Therefore, developing targeted interventions to strengthen psychological resilience in older adults is crucial for promoting cognitive health and successful aging, especially for those who have unhealthy lifestyles and with poorer health conditions.