bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: IL-6 signaling promotes DNA repair and prevents apoptosis in CD133+ stem-like cells of lung cancer after radiation
Authors: Chen Yuhchyau , Zhang Fuquan , Tsai Ying , Yang Xiadong , Yang Li , Duan Shanzhou , Wang Xin , Keng Peter , Lee Soo Ok 
Published: November 2015

Abstract
Background
Local tumor control by standard fractionated radiotherapy (RT) remains poor because of tumor resistance to radiation (radioresistance). It has been suggested that cancer stem cells (CSCs) are more radioresistant than non-CSCs. In previous studies, we have shown IL-6 promotes self-renewal of CD133+ CSC-like cells. In this study, we investigated whether IL-6 plays roles not only in promoting self-renewal of CD133+ cells after radiation, but also in conferring radioresistance of CD133+ cells in NSCLC.

Materials and methods
To compare radiation sensitivity of CSCs and non-CSCs, CD133+ CSC-like and CD133- cell populations were isolated from two NSCLC cell lines, A549 and H157, by immunomagnetic separation and their sensitivities to ionizing radiation were investigated using the clonogenic survival assay. To further study the IL-6 effect on the radiosensitivity of CD133+ CSC-like cells, CD133+ cells were isolated from A549IL-6si/sc and H157IL-6si/sc cells whose intracellular IL-6 levels were manipulated via the lentiviral transduction with IL-6siRNA. Post-irradiation DNA damage was analyzed by γ-H2AX staining and Comet assay. Molecular mechanisms by which IL-6 regulates the molecules associated with DNA repair and anti-apoptosis after radiation were analyzed by Western blot and immunofluoresecence (IF) staining analyses.

Results
NSCLC CD133+ CSC-like cells were enriched upon radiation. Survival of NSCLC CD133+ cells after radiation was higher than that of CD133- cells. Survival of IL-6 expressing NSC LC CD133+ cells (sc) was higher than that of IL-6 knocked-down cells (IL-6si) after radiation. IL-6 played a role in protecting NSCLC CD133+ cells from radiation-induced DNA damage and apoptosis.

Conclusions
IL-6 signaling promotes DNA repair while protecting CD133+ CSC-like cells from apoptotic death after radiation for lung cancer. A combined therapy of radiation and agents that inhibit IL-6 signaling (or its downstream signaling) is suggested to reduce CSC-mediated radioresistance in lung cancer.
Primary care referral to multidisciplinary high risk foot services – too few, too late
Authors: Plusch D , Penkala S , Dickson HG , Malone M 
Published: November 2015

AbstractBackgroundTo determine if patients with no contact with a multi‐disciplinary team High Risk Foot Service (MDT‐HRFS) had an increased rate of hospital admission for diabetes foot infection compared to patients with contact. Secondary aims were to report on clinical outcomes.MethodsA retrospective study was conducted at a major tertiary referral hospital in metropolitan Sydney over 12 months. An ICD‐10 search of the electronic medical record system and paper medical charts identified patients with diabetes mellitus (type 1 or type 2) and a primary admission for diabetes foot infection (DFI). Patients were categorised as having contact or no contact with an MDT‐HRFS.ResultsOne hundred ninety‐six hospital admissions (156 patients) were identified with DFI over a 12‐month period. Patients with no contact with a MDT‐HRFS represented three quarters of admissions (no contact = 116, 74.7 % vs. contact = 40, 25.6 %, p = 0.0001) and presented with more severe infection (no contact = 39 vs. contact = 12). The odds of lower extremity amputation increased five‐fold when both no contact and severe infection occurred in combination (no contact with severe infection and amputation = 34, 82.9 % vs. contact with severe infection and amputation = 7, 17.1 %, OR 4.9, 95 % CI 1.1–21.4, p = 0.037). Using estimates of both the contact and no contact population of people with diabetes and high‐risk feet and assuming the risk of amputation was the same, than the number of expected amputations in the no contact group should have been 55, however the observed number was 77, 22 more than expected (p = 0.0001).ConclusionsPatients with no contact with a MDT‐HRFS represented the majority of admissions for DFIs to a tertiary referral hospital. This group on population estimates appears to be at high risk of amputation of the lower extremity and therefore early referral of this high‐risk group might lower this risk.
Development of a therapy against metastatic bladder cancer using an interleukin-2 surface-modified MB49 bladder cancer stem cells vaccine
Authors: Zhu Yong-tong , Pang Shi-yu , Lei Cheng-yong , Luo Yang , Chu Qing-jun , Tan Wan-long 
Published: November 2015

Abstract
Introduction
In previous study the streptavidin interleukin-2 (SA-IL-2)-modified MB49 vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate MB49 bladder cancer stem cells (MCSCs). Accordingly, we developed a SA-IL-2-modified MCSCs vaccine and evaluated its antitumor effects.

Methods
MCSCs were isolated and identified in cancer stem cells (CSCs) characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo. The SA-IL-2 MCSCs vaccine was prepared and its bioactivity was evaluated. The protective, therapeutic, specific and memory immune response in animal experiments were designed to identify whether the vaccine elicited antitumor immunity and acted against metastatic bladder cancer.

Results
MCSCs had higher level of CD133 and CD44, less susceptibility to chemotherapy, more pronounced migration and greater tumorigenic ability. The successfully prepared SA-IL-2 MCSCs vaccine inhibited the tumor volume and prolonged mice survival in animal experiments. The expression of IgG, the population of dendritic cells, CD8+ and CD4+ T cells were highest in the experimental group than in the four control groups.

Conclusions
The SA-IL-2 MCSCs vaccine induced an antitumor immune response and was used to eliminate MCSCs to prevent tumor regrowth.
An intermixed algorithm for strict pseudo-contractions in Hilbert spaces
Authors: Yao Zhangsong , Kang Shin Min , Li Hong-Jun 
Published: November 2015

AbstractAn intermixed algorithm for two strict pseudo-contractions in Hilbert spaces have been presented. It is shown that the suggested algorithms converge strongly to the fixed points of two strict pseudo-contractions, independently. As a special case, we can find the common fixed points of two strict pseudo-contractions in Hilbert spaces.
Existence of best proximity points for controlled proximal contraction
Authors: Kiran Quanita , Ali Muhammad Usman , Kamran Tayyab , Karapınar Erdal 
Published: November 2015

AbstractIn this paper, we investigate the sufficient condition for the existence of best proximity points for non-self-multivalued mappings. Additionally, we discuss the stability theorem for such mappings. Our results improve and generalize some existing results on the topic in the literature, in particular, the results of Lim and of Abkar and Gabeleh.
Self-adaptive algorithms for proximal split feasibility problems and strong convergence analysis
Authors: Yao Yonghong , Yao Zhangsong , Abdou Afrah AN , Cho Yeol Je 
Published: November 2015

AbstractThe purpose of the paper is to study the proximal split feasibility problems. For solving the problems, we present new self-adaptive algorithms with the regularization technique. By using these algorithms, we give some strong convergence theorems for the proximal split feasibility problems.
A Gaussian function model for simulation of complex environmental sensing
Authors: Chaudhry Qasim Ali 
Published: November 2015

Abstract

Background
Sensors can be used to sense not only simple behavior but also complex ones. Previous work has demonstrated how agent-based modeling can be used to model sensing of complex behavior in Complex Environments.

Findings
Here, we propose a mathematical model using Gaussian function for the previously developed Agent Based Model (ABM) for Sensing of Emergent behavior in Complex Adaptive System (SECAS). The goodness of the fitted curve was observed by using standard tools, e.g. by determining SSE, SSM, ASSM and RMSE.

Conclusions
Our proposed model provides a good fit for data obtained from the earlier model. Also the developed model provides a bench mark against the data obtained from a former Agent Based Model.
Repeated Treatment with Bone Marrow Cell Secretory Products Maintains Long-Term Renoprotection in Experimental Chronic Kidney Disease: A Placebo-Controlled Trial
Authors: Yuen Darren A. , Kepecs David M. , Zhang Yanling , Advani Suzanne , Thai Kerri , Connelly Kim A. , Gilbert Richard E. 
Published: November 2015

Background: Bone marrow-derived early outgrowth cells (EOCs) secrete soluble factors that exert potent renoprotective effects, such that infusion of their conditioned medium recapitulates the affects of the cells themselves. Objectives: The objective of this study is to test whether the protective effect of conditioned medium infusion wanes with time and whether tachyphylaxis occurs with repeated administration. Design: This is a placebo-controlled animal study. Setting: The study was conducted at St. Michael's Hospital, Toronto, Ontario, Canada. Subjects: Fischer 344 (F344) rats were used in this study. Measurements: The following were measured: (1) urinary protein:creatinine ratio, (2) glomerular filtration rate, (3) systolic blood pressure, (4) body weight, (5) glomerular endothelial cell density, and (6) glomerular and tubulointerstitial type IV collagen deposition. Methods: Subtotally nephrectomized F344 rats, a model of progressive chronic kidney disease, were randomized 4 weeks post-surgery to receive thrice-weekly intravenous injections of concentrated EOC-conditioned medium (EOC CM) or unconditioned medium (UCM) over 2 weeks. Three animal groups were studied, according to whether they were administered conditioned medium: once (Initial Therapy Only group), twice (Repeat Therapy group), or not at all (No Therapy group). Results: Following initial therapy, EOC CM-treated animals excreted less urinary protein, a marker of renal injury, than their UCM-treated counterparts. At 10 weeks post-subtotal nephrectomy, however, mean urinary protein excretion in conditioned medium-treated animals was fourfold greater than at the completion of the initial treatment course. At this time point, conditioned medium-treated animals were randomized to receive a second course of either conditioned medium (Repeat Therapy group) or unconditioned medium (Initial Therapy Only group). At study end (14 weeks post-subtotal nephrectomy), Repeat Therapy animals demonstrated higher glomerular filtration rate, less proteinuria, preserved renal microvasculature, and diminished fibrosis when compared with the No Therapy group. Initial Therapy Only animals exhibited an intermediate effect. Limitations: Testing the effect of EOC-conditioned medium in a single model of chronic kidney disease (CKD) has limitations. Conclusions: These findings suggest that early outgrowth cell-derived factors, while renoprotective, have a limited duration of action. Repeated administration of these factors, however, is able to extend the duration of efficacy and attenuate the progression of experimental chronic kidney disease.
Genome sequence and description of Pantoea septica strain FF5
Authors: Lo Cheikh Ibrahima , Padhmanabhan Roshan , Mediannikov Oleg , Nguyen Thi Tien , Raoult Didier , Fournier Pierre-Edouard , Fenollar Florence 
Published: November 2015

AbstractStrain FF5 was isolated from the skin flora of a healthy Senegalese 35-year-old woman. This strain was identified as belonging to the species Pantoea septica based on rpoB sequence identity of 99.7 % with Pantoea septica strain LMG 5345T and a highest MALDI-TOF-MS score of 2.3 with Pantoea septica. Like P. septica, this FF5 strain is a Gram-negative, aerobic, motile, and rod-shaped bacterium. Currently, 17 genomes have been sequenced within the genus Pantoea but none for Pantoea septica. Herein, we compared the genomic properties of strain FF5 to those of other species within the genus Pantoea. The genome of this strain is 4,548,444 bp in length (1 chromosome, no plasmid) with a G + C content of 59.1 % containing 4125 protein-coding and 68 RNA genes (including 2 rRNA operons). We also performed an extensive phenotypic analysis showing new phenotypic characteristics such as the production of alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase.
Institutional Knots: A Comparative Analysis of Cord Blood Policy in Canada and the United States
Authors: Denburg Avram 
Published: November 2015

AbstractUmbilical cord blood is a rich source of blood stem cells, which are of critical clinical importance in the treatment of a variety of malignant and genetic conditions requiring stem cell transplantation. Many countries have established national public cord blood banks; such banks often coexist with a panoply of private options for cord blood banking. Until recently, Canada was the only G8 country without a national cord blood bank. This differs markedly from the United States, which years ago established a national cord blood bank policy and inventory. This article investigates potential reasons for this discrepancy through a comparative analysis of the evolution of programs and policies on national cord blood banking in Canada and the United States. My analysis suggests that cross-national discrepancies in policy on public cord blood banking were determined primarily by institutional factors, principal among them formal governmental structure and the legacy of past policies. Institutional entrepreneurialism in the health sector played a constitutive role in the earlier evolution of national cord blood policy in the United States as compared to Canada.
In the Name of Population Well-Being: The Case for Public Health Surveillance
Authors: Fairchild Amy L. , Bayer Ronald 
Published: November 2015

AbstractSurveillance is the radar of public health. Without tracking, often by name, the incidence and prevalence of both infectious and chronic disease, health officials would be unable to understand where and how to potentially intervene or what resources might be required to protect populations. Surveillance without individual informed consent has been challenged in the name of both bioethics and human rights. In this article we contend that a robust conception of public health not only justifies surveillance but, without disregarding the need to respect individuals, provides an affirmative duty to engage in surveillance. There may be social and political circumstances in which the names of those reported cannot be protected from unwarranted disclosure and misuse for ends that have little to do with protecting the public's health. But while the potential for misuse requires an ongoing, searching scrutiny of disease surveillance, remote or hypothetical threats should not serve to undermine this vital public health activity.
(Public) Health and Human Rights in Practice
Authors: Annas George J. , Mariner Wendy K. 
Published: November 2015

AbstractPublic health's reliance on law to define and carry out public activities makes it impossible to define a set of ethical principles unique to public health. Public health ethics must be encompassed within—and consistent with—a broader set of principles that define the power and limits of governmental institutions. These include human rights, health law, and even medical ethics. The human right to health requires governments not only to respect individual human rights and personal freedoms, but also, importantly, to protect people from harm from external sources and third parties, and to fulfill the health needs of the population. Even if human rights are the natural language for public health, not all public health professionals are comfortable with the language of human rights. Some argue that individual human rights—such as autonomy and privacy—unfairly limit the permissible means to achieve the goal of health protection. We argue that public health should welcome and promote the human rights framework. In almost every instance, this will make public health more effective in the long run, because the goals of public health and human rights are the same: to promote human flourishing.
ATM and ATR signaling at a glance
Authors: Awasthi Poorwa , Foiani Marco , Kumar Amit 
Published: November 2015

ABSTRACTATM and ATR signaling pathways are well conserved throughout evolution and are central to the maintenance of genome integrity. Although the role of both ATM and ATR in DNA repair, cell cycle regulation and apoptosis have been well studied, both still remain in the focus of current research activities owing to their role in cancer. Recent advances in the field suggest that these proteins have an additional function in maintaining cellular homeostasis under both stressed and non-stressed conditions. In this Cell Science at a Glance article and the accompanying poster, we present an overview of recent advances in ATR and ATM research with emphasis on that into the modes of ATM and ATR activation, the different signaling pathways they participate in – including those that do not involve DNA damage – and highlight their relevance in cancer.
Impaired mitochondrial Fe-S cluster biogenesis activates the DNA damage response through different signaling mediators
Authors: Pijuan Jordi , María Carlos , Herrero Enrique , Bellí Gemma 
Published: November 2015

ABSTRACT
Fe-S cluster biogenesis machinery is required for multiple DNA metabolism processes. In this work, we show that, in Saccharomyces cerevisiae, defects at different stages of the mitochondrial Fe-S cluster assembly machinery (ISC) result in increased spontaneous mutation rate and hyper-recombination, accompanied by an increment in Rad52-associated DNA repair foci and a higher phosphorylated state of γH2A histone, altogether supporting the presence of constitutive DNA lesions. Furthermore, ISC assembly machinery deficiency elicits a DNA damage response that upregulates ribonucleotide reductase activity by promoting the reduction of Sml1 levels and the cytosolic redistribution of Rnr2 and Rnr4 enzyme subunits. Depending on the impaired stage of the ISC machinery, different signaling pathway mediators contribute to such a response, converging on Dun1. Thus, cells lacking the glutaredoxin Grx5, which are compromised at the core ISC system, show Mec1- and Rad53-independent Dun1 activation, whereas both Mec1 and Chk1 are required when the non-core ISC member Iba57 is absent. Grx5-null cells exhibit a strong dependence on the error-free post-replication repair and the homologous recombination pathways, demonstrating that a DNA damage response needs to be activated upon ISC impairment to preserve cell viability.
Structure and function of longin SNAREs
Authors: Daste Frédéric , Galli Thierry , Tareste David 
Published: November 2015

ABSTRACT
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins constitute the core membrane fusion machinery of intracellular transport and intercellular communication. A little more than ten years ago, it was proposed that the long N-terminal domain of a subset of SNAREs, henceforth called the longin domain, could be a crucial regulator with multiple functions in membrane trafficking. Structural, biochemical and cell biology studies have now produced a large set of data that support this hypothesis and indicate a role for the longin domain in regulating the sorting and activity of SNAREs. Here, we review the first decade of structure–function data on the three prototypical longin SNAREs: Ykt6, VAMP7 and Sec22b. We will, in particular, highlight the conserved molecular mechanisms that allow longin domains to fold back onto the fusion-inducing SNARE coiled-coil domain, thereby inhibiting membrane fusion, and describe the interactions of longin SNAREs with proteins that regulate their intracellular sorting. This dual function of the longin domain in regulating both the membrane localization and membrane fusion activity of SNAREs points to its role as a key regulatory module of intracellular trafficking.
On doing two things at once: dolphin brain and nose coordinate sonar clicks, buzzes, and emotional squeals with social sounds during fish capture
Authors: Ridgway Sam , Samuelson Dianna , Van Alstyne Kaitlin , Price DruAnn 
Published: November 2015

Dolphins fishing alone in open waters may whistle without interrupting their sonar clicks as they find and eat or reject fish. Our study is the first to match sound and video from the dolphin with sound and video from near the fish. During search and capture of fish, free-swimming dolphins carried cameras to record video and sound. A hydrophone in the far field near the fish also recorded sound. From these two perspectives, we studied the time course of dolphin sound production during fish capture. Our observations identify the instant of fish capture. There are three consistent acoustic phases: sonar clicks locate the fish; bout 0.4 sec before capture, the dolphin clicks become more rapid to form a second phase, the terminal buzz; at or just before capture, the buzz turns to an emotional squeal-the victory squeal, which may last 0.2 to 20 sec after capture. The squeals are pulse bursts that vary in duration, peak frequency, and amplitude. The victory squeal may be a reflection of emotion triggered by brain dopamine release. It may also affect prey to ease capture and or it may be a way to communicate the presence of food to other dolphins.Dolphins also use whistles as communication or social sounds. Whistling during sonar clicking suggests that dolphins may be adept at doing two things at once. We know that dolphin brain hemispheres may sleep independently. Our results suggest that the two dolphin brain hemispheres may also act independently in communication.
Directed Graphs and Induced Magnetic Multipoles in Polycyclic Hydrocarbons
Authors: Ceulemans Arnout , Arvanitidis Athanasios G 
Published: November 2015

Abstract
Time-odd interactions in molecules may give rise to a current along the bonds connecting the atoms. In a graph representation of a molecule the analogue of these currents corresponds to directed edges. We consider the distribution of currents in polycyclic molecules, using a minimal cycle basis. The irreducible representations of this basis in the automorphism group of the molecular graph delineate specific magnetic multipoles. This is illustrated for the case of corazulene with fourfold symmetry which may sustain a magnetic octopole. It is further shown how breaking of the fourfold symmetry can give rise to the induction of an octopolar magnetic moment by a homogeneous magnetic field. Two conjugated polycyclic hydrocarbon systems showing an induced octopolar moment are investigated by ab initio current density calculations.
Sequential Structural Control of Open-Framework Nanoparticles Both in Dispersion and in Film for Electrochemical Performance Tuning
Authors: Lee Kyoung-Moo , Takahashi Akira , Tanaka Hisashi , Kim Kyung Ho , Kawamura Midori , Abe Yoshio , Ishizaki Manabu , Kurihara Masato , Kawamoto Tohru 
Published: November 2015

Abstract
We demonstrated that the sequential structural control of nanoparticles of an open-framework coordination polymer, copper hexacyanoferrate (CuHCF). The structural control has been achieved by adding the component ions not only in dispersion phase but also in the thin film form. The CuHCF nanoparticles (NPs) in the dispersion phase changed chemical composition and enhanced dispersibility with capturing [Fe(CN)6]3− anions. On the other hand, the NPs in the thin film were reversibly turned to the original state by capturing Cu2+ cations. Through the sequential processes, all of the synthesized CuHCF NPs were utilized for the high-quality electrochemical electrode for without electrochemical side reaction. This process will contribute the preparation of various electrochemical devices such as electrochromic devices, secondary batteries and biosensors.