bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performativity in Action: How Risk Communication Interacts in Risk Regulation
Authors: Marijn Poortvliet P. , Duineveld Martijn , Purnhagen Kai 
Published: February 2017

Examples abound of highly politicized instances of risk controversies, such as the climate debate, counterterrorism, and the commercialization of genetically modified organisms (GMOs). Numerous reasons, such as divergent perspectives, ways of communication, and interests, explain why involved actors often find themselves locked in a controversy.For instance, in the GMO debates environmental politicians, NGOs, industrial parties, consumers, and GMO scientists have exerted very distinct ways of communication, resulting in a highly polarized and contested gene-risk landscape. As a consequence, some industrial players have left or terminated R&D activities in the EU, while other scientists escape the gaze of EU-regulations and started experimenting in places with a different approach to GMO regulation and control.
Identification of cell-type-specific mutations in nodal T-cell lymphomas
Authors: Nguyen T B , Sakata-Yanagimoto M , Asabe Y , Matsubara D , Kano J , Yoshida K , Shiraishi Y , Chiba K , Tanaka H , Miyano S , Izutsu K , Nakamura N , Takeuchi K , Miyoshi H , Ohshima K , Minowa T , Ogawa S , Noguchi M , Chiba S 
Published: February 2017

AbstractRecent genetic analysis has identified frequent mutations in ten-eleven translocation 2 (TET2), DNA methyltransferase 3A (DNMT3A), isocitrate dehydrogenase 2 (IDH2) and ras homolog family member A (RHOA) in nodal T-cell lymphomas, including angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, not otherwise specified. We examined the distribution of mutations in these subtypes of mature T-/natural killer cell neoplasms to determine their clonal architecture. Targeted sequencing was performed for 71 genes in tumor-derived DNA of 87 cases. The mutations were then analyzed in a programmed death-1 (PD1)-positive population enriched with tumor cells and CD20-positive B cells purified by laser microdissection from 19 cases. TET2 and DNMT3A mutations were identified in both the PD1+ cells and the CD20+ cells in 15/16 and 4/7 cases, respectively. All the RHOA and IDH2 mutations were confined to the PD1+ cells, indicating that some, including RHOA and IDH2 mutations, being specific events in tumor cells. Notably, we found that all NOTCH1 mutations were detected only in the CD20+ cells. In conclusion, we identified both B- as well as T-cell-specific mutations, and mutations common to both T and B cells. These findings indicate the expansion of a clone after multistep and multilineal acquisition of gene mutations.
Host-inherent variability influences the transcriptional response of Staphylococcus aureus during in vivo infection
Authors: Thänert Robert , Goldmann Oliver , Beineke Andreas , Medina Eva 
Published: February 2017

AbstractThe rise of antibiotic resistance calls for alternative strategies to treat bacterial infections. One attractive strategy is to directly target bacterial virulence factors with anti-virulence drugs. The expression of virulence traits by pathogens is, however, not constitutive but rather induced by the level of stress encountered within the host. Here we use dual RNA sequencing (RNA-seq) to show that intrinsic variability in the level of host resistance greatly affects the pathogen's transcriptome in vivo. Through analysis of the transcriptional profiles of host and pathogen during Staphylococcus aureus infection of two mouse strains, shown to be susceptible (A/J) or resistant (C57BL/6) to the pathogen, we demonstrate that the expression of virulence factors is dependent on the encountered host resistance. We furthermore provide evidence that this dependence strongly influences the efficacy of anti-virulence strategies, highlighting a potential limitation for the implementation of these strategies.
Ultrahigh-resolution imaging of water networks by atomic force microscopy
Authors: Shiotari Akitoshi , Sugimoto Yoshiaki 
Published: February 2017

AbstractLocal defects in water layers growing on metal surfaces have a key influence on the wetting process at the surfaces; however, such minor structures are undetectable by macroscopic methods. Here, we demonstrate ultrahigh-resolution imaging of single water layers on a copper(110) surface by using non-contact atomic force microscopy (AFM) with molecular functionalized tips at 4.8 K. AFM with a probe tip terminated by carbon monoxide predominantly images oxygen atoms, whereas the contribution of hydrogen atoms is modest. Oxygen skeletons in the AFM images reveal that the water networks containing local defects and edges are composed of pentagonal and hexagonal rings. The results reinforce the applicability of AFM to characterize atomic structures of weakly bonded molecular assemblies.
Solvothermal synthesis of CdIn2S4 photocatalyst for selective photosynthesis of organic aromatic compounds under visible light
Authors: Ling Cancan , Ye Xiangju , Zhang Jinghu , Zhang Jinfeng , Zhang Sujuan , Meng Sugang , Fu Xianliang , Chen Shifu 
Published: February 2017

AbstractTernary chalcogenide semiconductor, cadmium indium sulfide (CdIn2S4), was prepared by a simple solvothermal method using ethylene glycol as a solvent, as well as indium chloride tetrahydrate (InCl3.4H2O), cadmium nitrate tetrahydrate [Cd(NO3)2.4H2O], and thiacetamide (TAA) as precursors. The resulted sample was subject to a series of characterizations. It is the first time to use CdIn2S4 sample as a visible light-driven photocatalyst for simultaneous selective redox transformation of organic aromatic compounds. The results indicate that the as-synthesized CdIn2S4 photocatalyst not only has excellent photocatalytic performance compared with pure In2S3 and CdS for the selective oxidation of aromatic alcohols in an oxygen environment, but also shows high photocatalytic redox activities under nitrogen atmosphere. A possible mechanism for the photocatalytic redox reaction in the coupled system was proposed. It is hoped that our current work could extend the applications of CdIn2S4 photocatalyst and provide new insights for selective transformations of organic compounds.
Signatures of malaria-associated pathology revealed by high-resolution whole-blood transcriptomics in a rodent model of malaria
Authors: Lin Jing-wen , Sodenkamp Jan , Cunningham Deirdre , Deroost Katrien , Tshitenge Tshibuayi Christine , McLaughlin Sarah , Lamb Tracey J. , Spencer-Dene Bradley , Hosking Caroline , Ramesar Jai , Janse Chris J. , Graham Christine , O’Garra Anne , Langhorne Jean 
Published: February 2017

AbstractThe influence of parasite genetic factors on immune responses and development of severe pathology of malaria is largely unknown. In this study, we performed genome-wide transcriptomic profiling of mouse whole blood during blood-stage infections of two strains of the rodent malaria parasite Plasmodium chabaudi that differ in virulence. We identified several transcriptomic signatures associated with the virulent infection, including signatures for platelet aggregation, stronger and prolonged anemia and lung inflammation. The first two signatures were detected prior to pathology. The anemia signature indicated deregulation of host erythropoiesis, and the lung inflammation signature was linked to increased neutrophil infiltration, more cell death and greater parasite sequestration in the lungs. This comparative whole-blood transcriptomics profiling of virulent and avirulent malaria shows the validity of this approach to inform severity of the infection and provide insight into pathogenic mechanisms.
Conserved gene regulation during acute inflammation between zebrafish and mammals
Authors: Forn-Cuní G. , Varela M. , Pereiro P. , Novoa B. , Figueras A. 
Published: February 2017

AbstractZebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases. However, in a context where even mice have been questioned as a valid model for these analysis, a systematic study evaluating the reproducibility of human and mammalian inflammatory diseases in zebrafish is still lacking. In this report, we characterize the transcriptomic regulation to lipopolysaccharide in adult zebrafish kidney, liver, and muscle tissues using microarrays and demonstrate how the zebrafish genomic responses can effectively reproduce the mammalian inflammatory process induced by acute endotoxin stress. We provide evidence that immune signaling pathways and single gene expression is well conserved throughout evolution and that the zebrafish and mammal acute genomic responses after lipopolysaccharide stimulation are highly correlated despite the differential susceptibility between species to that compound. Therefore, we formally confirm that zebrafish inflammatory models are suited to study the basic mechanisms of inflammation in human inflammatory diseases, with great translational impact potential.
Epigenetic origin of evolutionary novel centromeres
Authors: Tolomeo Doron , Capozzi Oronzo , Stanyon Roscoe R. , Archidiacono Nicoletta , D’Addabbo Pietro , Catacchio Claudia R. , Purgato Stefania , Perini Giovanni , Schempp Werner , Huddleston John , Malig Maika , Eichler Evan E. , Rocchi Mariano 
Published: February 2017

AbstractMost evolutionary new centromeres (ENC) are composed of large arrays of satellite DNA and surrounded by segmental duplications. However, the hypothesis is that ENCs are seeded in an anonymous sequence and only over time have acquired the complexity of “normal” centromeres. Up to now evidence to test this hypothesis was lacking. We recently discovered that the well-known polymorphism of orangutan chromosome 12 was due to the presence of an ENC. We sequenced the genome of an orangutan homozygous for the ENC, and we focused our analysis on the comparison of the ENC domain with respect to its wild type counterpart. No significant variations were found. This finding is the first clear evidence that ENC seedings are epigenetic in nature. The compaction of the ENC domain was found significantly higher than the corresponding WT region and, interestingly, the expression of the only gene embedded in the region was significantly repressed.
INFLUENZA DETECTION: A PROSPECTIVE COMPARISON OF SURVEILLANCE METHODS AND ANALYSIS OF ISOLATES
Authors: MARINE WILLIAM M. , McGOWAN JOHN , THOMAS JAMES E. 
Published: February 2017

Abstract
Several prospective methods for surveillance of influenza were compared for the speed and efficacy with which they identified cases of influenza in metropolitan Atlanta during 1972–1975, a period in which two outbreaks of influenza occurred. In a large city-county hospital's outpatient clinics, systematic collection of pharyngeal cultures for virus isolation provided a rapid means for discovering the presence of influenzal illness and for monitoring the extent of the outbreak. Both obtaining cultures and determining the percentage of patients with respiratory complaints indicated an outbreak 3–5 weeks before epidemic disease was confirmed by a rise in the number of deaths due to pneumonia and influenza. Antigenic analysis of influenza A strains isolated during this three-year period showed that influenza A strains recovered in Atlanta in 1973–1974 (a non-epidemic year) were identical to the strains recovered in the 1974–1975 epidemic. Why no epidemic occurred in 1973–1974 in the presence of the epidemic strain of the virus is unknown, and the lack of an epidemic suggests that the role of virus persistence in the epidemiology of influenza requires further evaluation.
ORAL RABIES VACCINE: EVALUATION OF ITS INFECTIVITY IN THREE SPECIES OF RODENTS
Authors: WINKLER WILLIAM G. , SHADDOCK JOHN H. , WILLIAMS L. W. 
Published: February 2017

Abstract
The safety of oral rabies vaccine was evaluated in 3 species of rodents, ani-mals which might inadvertently consume field-distributed vaccine baits intended for foxes. The potentials for infection from bait ingestion and transmission through cannibalism were evaluated. Results indicate that ingestion of the vaccine baits used in these experiments will result in infection and disease ofsome rodents. Of 64 rodents which ate baits. 19 (30%) died of clinical rabies; the diagnosis was confirmed in 11 (17%), and 2 (3%) had demonstrable rabies virus in their salivary glands. Only 3 of 112 (3%) rodents that ate vaccine-infected rodents contracted rabies, and none had demonstrable virus in their salivary glands.
Symptomatic, Inspiration‐Induced Atrioventricular Block: Treated with Pacemaker Implant
Authors: HOFFMANN DIEGO A. , ATAMAÑUK ANDRÉS NICOLÁS , RUIZ GRACIELA AURORA , CHIRIFE RAUL , TENTORI MARIA CRISTINA , SALZBERG SIMÓN 
Published: February 2017

BackgroundThe respiratory sinus arrhythmia is the most common cardiac arrhythmia. It consists of phasic variations of sinus cycle length produced by an autonomic reflex, and is characterized by increased heart rate during inspiration and decline in expiration. This phenomenon is due to a reduction of the parasympathetic tone during inspiration, causing a rise in the heart rate, while during expiration the opposite phenomenon occurs. This arrhythmia is more frequent in young adults, although it can also be seen in any age group.Methods and ResultsWe report the case of a 46‐year‐old female, who was transferred to our institution because of dizziness and loss of balance reported during deep inspiration, associated with sinus bradycardia and atrioventricular block.
‘I need complete trust in nurses’ – home mechanical ventilated patients’ perceptions of safety
Authors: Schaepe Christiane , Ewers Michael 
Published: February 2017

BackgroundAlthough home care has advanced over the last few decades, little research on patient safety has been carried out in this setting. Furthermore, it is unclear how patients perceive their situation and safety. The insiders’ views might be especially relevant for technology‐dependent individuals, such as users of home mechanical ventilation (HMV).AimThe aim of this study was to examine how HMV patients perceive their situation and what makes them feel safe or unsafe.DesignExplorative qualitative study.MethodsData were collected in two regions in Germany between April and December 2014 by means of semi‐structured interviews with 21 HMV patients. Thematic analysis was used to analyse data.FindingsThree themes emerged: the meaning of an interpersonal relationship between the nurse and HMV patient is expressed in the theme Being familiar – Having trust. The importance of the attentiveness of nurses for the patients’ feeling of safety is described in the theme Being able to communicate – Being noticed. The theme Experiencing continuity – Feeling presence points to the organisational dimension of HMV care provision.ConclusionsThe interpersonal nurse–patient relationship plays a key role in promoting HMV patients’ feeling of safety. Thus, HMV patients have a relational approach to safety. In order to enhance the patients’ feeling of safety, nurses should strive to develop a trusting relationship with patients and demonstrate their presence and attentiveness. Regarding the provision of care, competent and continuous care should be made a priority.
Pau Estorch Siqués (1805–71) and his ‘magnes venenorum’
Authors: Sabaté Casellas F. , Torres Gallardo B. 
Published: February 2017

Abstract
From ancient times, many efforts have been made to find a substance with the capacity to neutralize the toxic substances or poisons that can affect the human body. Such poisons of animal, vegetable or mineral origin enter the body through the skin, digestive or respiratory systems. Many simple or complex substances have been investigated. Estorch Siqués was a multitalented personality interested in literature, public health affairs and practical medicine; he was also a businessman who publicized and sold his own products. His ‘magnes venenorum’ is another example of the search for a universal antidote during the second half of the nineteenth century.
Sentidos do trabalho e formas de participação: o caso de uma cooperativa de trabalhadores rurais do Assentamento Mário Lago, Ribeirão Preto (SP)
Authors: Melo Thainara Granero de , Scopinho Rosemeire Aparecida 
Published: February 2017

O problema da participação em cooperativas de trabalhadores rurais assentados têm sido estudado sob diferentes perspectivas, porém não há consenso na literatura. De todo modo, a literatura mostra que há diferentes fatores que contribuem para a falta de participação real dos trabalhadores, especialmente porque o acesso às políticas públicas está condicionado à criação de cooperativas ou associações, o que torna compulsório o associativismo. O objetivo deste artigo é discutir as formas de participação de trabalhadores rurais em cooperativas por meio da análise dos sentidos atribuídos ao trabalho. Estudamos o caso de uma cooperativa do Assentamento Mário Lago, Ribeirão Preto (SP),por meio de estratégias de observações diretas e de entrevistas semiestruturadas. Constatamos que a participação instrumental predominava no grupo, reforçada por dependências materiais, institucionais e afetivas dos sujeitos. Os sentidos atribuídos pelos sujeitos ao trabalho cooperado reforçaram a constatação de que o modo como as políticas de desenvolvimento rural têm sido pensadas no país traduzem a desproporcionalidade entre a prática cotidiana da cooperação e o cooperativismo institucionalizado.
Prevention of Diet-Induced Metabolic Dysregulation, Inflammation, and Atherosclerosis in

Ldlr
−/−

Mice by Treatment With the ATP-Citrate Lyase Inhibitor Bempedoic Acid
Authors: Samsoondar Joshua P. , Burke Amy C. , Sutherland Brian G. , Telford Dawn E. , Sawyez Cynthia G. , Edwards Jane Y. , Pinkosky Stephen L. , Newton Roger S. , Huff Murray W. 
Published: February 2017

Objective—
Bempedoic acid (ETC-1002, 8-hydroxy-2,2,14,14-tetramethylpentadecanedioic acid) is a novel low-density lipoprotein cholesterol–lowering compound. In animals, bempedoic acid targets the liver where it inhibits cholesterol and fatty acid synthesis through inhibition of ATP-citrate lyase and through activation of AMP-activated protein kinase. In this study, we tested the hypothesis that bempedoic acid would prevent diet-induced metabolic dysregulation, inflammation, and atherosclerosis.

Approach and Results—

Ldlr
−/−

mice were fed a high-fat, high-cholesterol diet (42% kcal fat, 0.2% cholesterol) supplemented with bempedoic acid at 0, 3, 10 and 30 mg/kg body weight/day. Treatment for 12 weeks dose-dependently attenuated diet-induced hypercholesterolemia, hypertriglyceridemia, hyperglycemia, hyperinsulinemia, fatty liver and obesity. Compared to high-fat, high-cholesterol alone, the addition of bempedoic acid decreased plasma triglyceride (up to 64%) and cholesterol (up to 50%) concentrations, and improved glucose tolerance. Adiposity was significantly reduced with treatment. In liver, bempedoic acid prevented cholesterol and triglyceride accumulation, which was associated with increased fatty acid oxidation and reduced fatty acid synthesis. Hepatic gene expression analysis revealed that treatment significantly increased expression of genes involved in fatty acid oxidation while suppressing inflammatory gene expression. In full-length aorta, bempedoic acid markedly suppressed cholesteryl ester accumulation, attenuated the expression of proinflammatory M1 genes and attenuated the
iNos
/
Arg1
ratio. Treatment robustly attenuated atherosclerotic lesion development in the aortic sinus by 44%, with beneficial changes in morphology, characteristic of earlier-stage lesions.

Conclusions—
Bempedoic acid effectively prevents plasma and tissue lipid elevations and attenuates the onset of inflammation, leading to the prevention of atherosclerotic lesion development in a mouse model of metabolic dysregulation.
Breach of Treaties in the Ancient Near East
Authors: Sulyok Gábor 
Published: February 2017

AbstractThe history of the breach of treaties can be traced back to the ancient Near East. The relative abundance and diversity of contemporary sources attest that the breaking of treaty obligations must have been a rather persistent problem, and that such occurrences were regarded as events of utmost importance throughout the Bronze and Iron Ages. The present study strives to demonstrate how peoples of old may have perceived and reacted to the breach of treaties on the basis of selected writings—the Legend of Etana, the Indictment of Madduwatta, the Indictment of Mita, the plague prayers of Mursili and the Old Testament—that provide, beyond the exposition of actual or alleged facts, a deeper insight into the psychological and procedural aspects of the subject.
Assessment of the Validity of Investing in Energy-Efficient Single-Family Construction in Poland - Case Study
Authors: Tomczak K. , Kinash O. 
Published: February 2017

AbstractThe article raised issues related to the design and execution of low-energy objects in Polish conditions. Based on the designed single-family house, adapted to the requirements of the National Fund for Environmental Protection and Water Management (“NF40” standard), the tools to assist investment decisions by investors were shown. An economic analysis and a multi-criteria analysis were performed using AHP method which had provided an answer to the question whether it is worthwhile to bear higher investment costs in order to adjust to the standards of energy-efficient buildings that fulfil a minimal energy consumption's requirements contained in Polish law. In addition, the variant of object that had optimal characteristics due to the different preferences of investors was indicated. This paper includes analysis and observations on the attempts to unify that part of the building sector, which so far is considered to be personalized, and objects in accordance with the corresponding idea are designed as “custom-made”.
Łazienkowski Bridge Fire in Warsaw – Structural Damage and Restoration Method
Authors: Zobel H. , Karwowski W. , Wróbel M. , Mossakowski P. 
Published: February 2017

AbstractOn the 14th of February, 2015, a huge fire broke out on Łazienkowski Bridge; a five span bridge, 423 m long and 28 m wide, built in the years 1972-74. It was a fully steel structure with four plate girders and orthotropic deck. The fire started under the first span during the replacement of wooden service decks. The next day, the Department of Bridges of the Warsaw University of Technology was designated to conduct an expertise material investigation, geometrical verification, and FEM model analysis. The subject of this paper concentrates on geometrical issues. The main difficulty of this task was the lack of full reference data regarding the bridge's original structure. The old design was incomplete and there was no actual surveying results for the undamaged structure. As a conclusion, some remarks focused on surveying measurements and on the final decision regarding this bridge are given. It was eventually exchanged into a brand new one and put into public use on the 28th of October, 2015.
Lean and Agile Management Synergy in Construction of High-Rise Office Building
Authors: Nowotarski P. , Pasławski J. 
Published: February 2017

AbstractThe aim of this article is to identify opportunities for using synergies obtained by incorporation of the two methods of management: Lean Management and Agile Management on the example of the process of column concreting. Despite the seemingly contradictory assumptions the two concepts complement each other in analysed example. The strategy is based on using the idea of “one piece flow” in accordance with the Lean Management which led to a reduction of costs due to increased turnover of formwork. At the same time the success of the project resulted in a significant dependence on the ability to provide a rapid response to changing conditions during in the maturation of concrete (depending on weather conditions, which can be expected on the basis of projections having different reliability). The simultaneous use of Lean and Agile Management allowed to achieve positive results for different scenarios of environment impact on the analysed process.