bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA‐binding protein Trx regulates alternative splicing and promotes metastasis of HCC via interacting with LINC00152
Authors: Teng Xiangnan , Shang Jin , Du Lingyao , Huang Wei , Wang Yonghong , Liu Miao , Ma Yuanji , Wang Ming , Tang Hong , Bai Lang 
Published: September 2024

AbstractBackgroundEpithelial‐mesenchymal transition (EMT) is central to HCC metastasis, in which RNA‐binding proteins (RBPs) play a key role.MethodsTo explore the role of RBPs in metastasis of hepatocellular carcinoma (HCC), whole transcriptome sequencing was conducted to identify differential RBPs between HCC with metastasis and HCC without metastasis. The influence of RBPs on metastasis of HCC was verified by in vitro and in vivo experiments. The interaction of RBPs with non‐coding RNAs was evaluated by RNA immunoprecipitation and pull‐down assays. RNA sequencing, whole‐genome sequencing, and alternative splicing analysis were further performed to clarify post‐transcriptional regulation mechanisms.ResultsWhole transcriptome sequencing results showed that expression of thioredoxin (Trx) was significantly upregulated in HCC patients with metastasis. Trx was also found to be associated with poor prognosis in HCC patients. Overexpression of Trx could promote migration and invasion of HCC cells in vitro and increase the rate of lung metastasis of HCC cells in vivo. Moreover, binding assays showed that Trx could bind to LINC00152. As a result, LINC00152 was verified to determine the pro‐metastasis function of Trx by knockdown assay. Furthermore, we revealed that Trx could regulate metastasis‐associated alternative splicing program. Specifically, angiopoietin 1 (ANGPT1) was the splicing target; the splicing isoform switching of ANGPT1 could activate the PI3K–Akt pathway, upregulate EMT‐associated proteins, and promote migration and invasion of HCC cells.ConclusionsWe found that Trx could interact with LINC00152 and promote HCC metastasis via regulating alternative splicing, indicating that Trx may serve as a novel therapeutic target for HCC treatment.
Do hematologic malignancies cause a barrier for sentinel lymph node mapping in endometrial cancer? A case report
Authors: Unal Ceren , Çekiç Sebile Güler , Bulutay Pınar , Topkara Şevval Berfin , Giray Burak 
Published: September 2024

AbstractSentinel lymph node (SLN) mapping has changed the way of lymph node assessment and provided a minimal invasive option that lessens the negative effects of a full lymphadenectomy, has become a crucial tool in the treatment of endometrial cancer. Chronic lymphocytic leukemia (CLL) and endometrial cancer diagnosis at the same is a rare condition. This case report presents a 66‐year‐old woman admitting to the hospital with the complaint of postmenopausal bleeding. After hysteroscopic evaluation and Magnetic Resonance imaging confirming the endometrial cancer, total laparoscopic hysterectomy and bilateral salphingo‐ooferectomy with SLN mapping operation was planned. In the preoperative preparation she was also diagnosed with CLL. Considering the possible lymphatic obstruction, the risk of SLN mapping failure was foreseen, yet still the operation was performed as planned. This report will highlight a case where endometrial cancer and CLL were identified simultaneously, along with successful SLN mapping.
Color of Wine: Development of Reference Grids/Templates for Sensory Analysis With Trained Assessors
Authors: Gomis‐Bellmunt Anna , Claret Anna , Pérez Elortondo Francisco José , Guerrero Luis 
Published: September 2024

AbstractColor of wine gives information on many aspects of the product, and it is one of the main parameters defining wine quality. Consequently, color is an important attribute described in the Designations of Origin (PDO)'s technical specifications. In this paper, color reference grids for color sensory evaluation of white, rosé and red wines (linked to a specific methodology and controlled illumination) are developed, presented, and validated. The color intensity and color hue parameters of 2353 wines of different types have been analyzed using these reference grids during the period 2021–2023. Considering the results obtained, the usefulness of having a double color measurement (color intensity and color hue) is questioned and discussed.
Recovered or Not Recovered? A First Person Narrative Case for Non‐Abstinent Recovery
Authors: Tomlinson Peter 
Published: September 2024

ABSTRACTPurposeThe purpose of this paper was to explore interlocking questions relating to how we deal with drugs and medication in crisis residential mental health settings. In particular, it examines issues related to overly medical and simplistic explanations of the relationship between cannabis and psychosis, the positive effects of drugs, the effects of having standard rules for diverse groups of people and the concept of non‐abstinent recovery.BackgroundI have used mental health and addiction services for 25 years and I have 6 years' experience as a peer worker, working with many people who use drugs.MethodThis is an opinion piece built around a first‐person narrative.KeypointsDrugs can have positive effects and an appreciation and understanding of these effects is essential to understanding persistent drug use. How can we integrate non‐abstinent recovery as a valid and positive choice of recovery pathway?
Novel phylogenetic analysis of the Mesozoic common gymnosperm Xenoxylon Gothan reveals close affinity with extant Podocarpaceae (Coniferales)
Authors: Xie Aowei , Low Shook Ling , Wang Yongdong , Tian Ning , Uhl Dieter 
Published: September 2024

AbstractFossil wood is one of the crucial proxies for understanding terrestrial vegetation composition and development in the Earth's history. The gymnosperm wood taxon Xenoxylon Gothan is a significant member of the Mesozoic flora. To date, more than 20 species of Xenoxylon have been described. However, its botanical affinities have remained enigmatic ever since it was described, over a century ago. Here we perform a phylogenetic analysis of Xenoxylon to understand the systematic relationship with extant conifers. Data come from four nucleotide regions (trnL‐F, trnK‐matK, rbcL, psbA‐trnH), xylological characters, and biomolecular composition of five extant conifer families; for Xenoxylon, no nucleotide data are available. Using maximum parsimony in Tree Analysis using New Technology, Xenoxylon appeared basal to Araucariaceae in the data set combining genes and xylological characters, whereas Xenoxylon is placed next to Podocarpaceae in the data set combining genes and biomolecular characters. To find a reliable systematic placement of Xenoxylon, a combined data set of genes, xylological and biomolecular characters is analyzed. Our results and interpretations indicate that Xenoxylon is closely related to Podocarpaceae. This first phylogenetic analysis of Xenoxylon fills the knowledge gap of the systematic relationship of this taxon and contributes to a better understanding of the evolution of extant Podocarpaceae.
“Another Kid on the Block”: Testing the Effects of Artificial Substrates on the Recruitment of Juvenile Fishes in the Northwestern Gulf of Mexico
Authors: Salinas Stefany , Cintra‐Buenrostro Carlos E. 
Published: September 2024

ABSTRACTMost artificial reefs (ARs) in the Gulf of Mexico are considered intermediate to high vertical relief structures which have recruited large predatory species indicating a lack of juvenile habitat. Small, inexpensive ARs, standard monitoring units for the recruitment of reef fishes (SMURFs), were deployed at eight treatment stations at −20 m as habitat for juvenile fishes to understand and determine the effects of substrate type on the recruitment of juvenile fishes. Each treatment station contained four SMURFs with four treatments: small and large concrete rubble (~10 and ~20 cm), oyster shells, and bare. Surveys conducted from July 2017 to July 2019 yielded 39 different juvenile species, including one of the most economically important species, Lutjanus campechanus, present across all replicates. There were 5238 individual fishes collected, and the family Lutjanidae accounted for ~49% of these. The highest species richness and diversity occurred in SMURFs containing oysters or small concrete rubble while bare treatment had the lowest. Both concrete rubble and oyster shells might offer shelter to numerous body shapes and sizes of juvenile fishes as a result from a variety of crevice sizes. This study suggests that the deployment of low‐relief structures with different substrate materials might affect recruitment of select species and provide further information useful to designing ARs, aiding fisheries production. Because enhancing fisheries is one of the goals of the Texas Parks and Wildlife Department Artificial Reef Program it is here recommended to continue developing other designs of low‐relief to be allocated in between existing high‐relief ARs that should serve as stepping stones for the survival of species of fisheries interest (e.g., L. campechanus).
Using formative research to enhance our understanding of implementation contexts: Preparing for a trial of maternal nutrition interventions
Authors: Thorne‐Lyman Andrew L. , Kalbarczyk Anna , Tumilowicz Alison , Christian Parul , Afsana Kaosar 
Published: September 2024

Key messages

Formative research to understand the sociocultural and economic context into which interventions are introduced can help to maximize the uptake of interventions both in the context of effectiveness trials and ultimately for nutrition programs.
Findings from the formative research study were used to inform different facets of the design of the effectiveness trial including providing evidence of the acceptability of the product, informing the decision to deliver the product to women's homes, and developing strategies to support adherence.
Global Insights and Trends in Research on Dermatophytes and Dermatophytosis: A Bibliometric Analysis
Authors: Ortiz Bryan , Ballesteros‐Monrreal Manuel G. , Rosales‐Tamashiro Juan , Bush Michelle , Salmanton‐García Jon , Fontecha Gustavo 
Published: September 2024

ABSTRACTBackgroundDermatophytosis, caused by dermatophytes, affects up to 25% of people globally, with higher rates observed in Africa and Asia. While these infections are usually superficial, they can become severe in immunocompromised individuals. Despite their high prevalence, scientific research on dermatophytes is limited and the epidemiological data available are insufficient. In addition, diagnostic methods are not standardised and there are challenges with resistance to antifungals.ObjectivesThis study aimed to conduct a bibliometric analysis of scientific publications related to dermatophytes and dermatophytosis to assess research output and trends.MethodsA bibliometric analysis of publications from 2000 to 2023 in Web of Science and Scopus examined trends, citation counts, publication types, key journals, top authors and institutions and funding sources.ResultsThe analysis revealed a significant increase in dermatophyte‐related publications, with 15,868 articles retrieved from the Web of Science and 23,189 from Scopus. Research articles dominated the output, constituting 76.2% in Web of Science and 80% in Scopus. Peak publication years were 2019, 2021 and 2022 in Web of Science, and 2020, 2021 and 2023 in Scopus, with lower output between 2000 and 2002. The United States and India were the leading contributors, followed by Brazil and China, though citation metrics varied. Although there has been a rise in the number of publications, the amount of research conducted on dermatophytes is still very limited in comparison with other types of fungal diseases.ConclusionsDermatophyte‐related research has increased over the past 2 decades. However, research gaps remain, particularly compared with other fungal diseases. Advances in diagnostics, antifungal testing and taxonomic classification are urgently needed. The study underscores the need for continued research and global collaboration to address these issues.
Herbal Amara extract induces gastric fundus relaxation via inhibition of the M2 muscarinic receptor
Authors: Piqué‐Borràs Maria‐Riera , Röhrl Johann , Künstle Gerald 
Published: September 2024

AbstractBackgroundImpaired gastric accommodation is one of the most frequent symptoms of functional dyspepsia. The safety and efficacy of conventional treatments remain to be proven and alternative herbal therapies have been proposed to alleviate gastrointestinal symptoms. This preclinical study examined the role of herbal Amara extract (containing Artemisia absinthium, Centaurium erythraea, Cichorium intybus, Gentiana lutea, Juniperus communis, Achillea millefolium, Peucedanum ostruthium, Salvia officinalis, and Taraxacum extracts) on gastric (fundus) accommodation and the possible implication of muscarinic receptors in its regulation.MethodsThe effect of Amara extract on fundus motility was investigated in organ baths of smooth muscle strips isolated from the fundus of guinea pigs, and the role of the muscarinic receptor pathway was evaluated using functional and radioligand binding assays in cell lines expressing the M2 or M3 muscarinic receptor.Key ResultsAmara extract inhibited carbachol‐induced contraction of guinea pig smooth muscle strips in a dose‐dependent manner. This relaxant effect was not affected by the M3 antagonist J‐104129. Amara extract also inhibited M2, but not M3, receptor activity in CHO‐K1 cells (IC50 219 μg mL−1), and specifically bound the M2 receptor (IC50 294 μg mL−1). Of the nine herbal components of Amara extract, Juniperus communis, P. ostruthium, and Salvia officinalis inhibited M2 receptor activity (IC50 32.0, 20.8, and 20.1 μg mL−1, respectively), and P. ostruthium was sufficient to reverse carbachol‐induced ex vivo contraction of guinea pig fundic smooth muscles.Conclusion and InferencesAmara extract relaxes gastric smooth muscles by inhibiting the M2 muscarinic receptor. This study suggests the potential benefit of Amara extract for patients with impaired gastric accommodation.
Pathogenic Germline Variants in Uveal Melanoma Driver and BAP1‐Associated Genes in Finnish Patients with Uveal Melanoma
Authors: Repo Pauliina E. , Jakkula Eveliina , Hiltunen Juho , Putkuri Heidi , Staskiewicz‐Tuikkanen Aleksandra , Järvinen Reetta‐Stiina , Täll Martin , Raivio Virpi , Al‐Jamal Rana'a T. , Kivelä Tero T. , Turunen Joni A. 
Published: September 2024

ABSTRACTUveal melanoma (UM) is a rare yet aggressive eye cancer causing over 50% mortality from metastasis. Familial UM, amounting to 1%–6% of patients in Finland and the United States, mostly lack identified genetic cause, while 8% show associations with other cancer syndromes. We searched novel genetic associations for predisposition to UM, additional to already studied BAP1 and MBD4, by using targeted amplicon sequencing of 19 genes associated with UM, BAP1, or renal cell carcinoma in 270 consecutively enrolled Finnish patients with UM. Key UM drivers GNAQ, GNA11, CYSLTR2, PLCB4, EIF1AX, and SF3B1 lacked pathogenic germline variants. One patient carried the pathogenic BRCA1 variant c.3626del p.(Leu1209*), and one harbored a novel truncating MET variant c.252C > G p.(Tyr84*), classified as likely pathogenic. FLCN and BRCA2, previously identified with pathogenic variants in patients with UM, did not have such variants in our cohort. Two patients were heterozygous for a pathogenic recessive BLM variant c.2824‐2A > T. None of the carriers of identified variants had familial UM. We identified BRCA1 and MET as genes with pathogenic germline variants in Finnish UM patients, each with a frequency of 0.4% (95% confidence interval, 0–2).
Network analysis of metabolomics, transcriptome and hormones reveals propionic acid‐mediated novel survival strategy against drought in wheat
Authors: Li Zongzhen , Lian Yanhao , Guo Hui , Li Chenxi , Ren Yongzhe , Xin Zeyu , Lin Tongbao , Wang Zhiqiang 
Published: September 2024

AbstractPropionic acid (PA), a low‐molecular‐weight organic acid, is crucial to plant life metabolism. However, the regulatory mechanism of PA‐mediated drought resistance in wheat remains largely unknown. Herein, we reported on a regulatory network of PA‐mediated drought resistance in wheat using integrated transcriptome and metabolomics analysis and verified genes associated with drought resistance. Compared to the water‐treated group, the application of PA alleviated the damage of drought by increasing plant water content, antioxidant enzyme activities and decreasing the malondialdehyde level (MDA). Transcriptome and metabolomics analysis revealed that PA triggered upregulation of key genes and metabolites, including TaBCAT, TaALDH6A1, TaALDH7A1, TaCHI, TaFLS, chrysin, and galangin, which were involved in valine, leucine and isoleucine degradation or flavonoid biosynthesis, respectively. In addition, the expression of genes encoding auxin‐related transcription factors (TFs) strikingly increased, such as auxin/indoleacetic acid (AUX/IAA) and auxin response factor (ARF). Moreover, PA activated abscisic acid (ABA) and indole‐3‐acetic acid (IAA) signalling pathways. Taken together, our findings suggest that PA promotes energy metabolism and antioxidant activities to confer wheat drought resistance by introducing comprehensive and systemic effects of valine, leucine and isoleucine degradation flavonoid biosynthesis. Furthermore, activated AUX/IAA and ARF TFs might serve vital roles in drought resistance via modulating IAA signalling. This study provides novel insights into PA‐mediated crop resistance and the improvement of the agroecological environment.
The module consisting of transcription factor WRKY14 and thaumatin‐like protein TLP25 is involved in winter adaptation in Ammopiptanthus mongolicus
Authors: Liu Qi , Zeng Weiying , Qi Lanting , Li Xuting , Zhou Yijun , Gao Fei 
Published: September 2024

AbstractThaumatin‐like proteins (TLPs) are conserved proteins involved in the defense and stress responses of plants. Previous studies showed that several TLPs were accumulated in leaf apoplast in Ammopiptanthus mongolicus in winter, indicating that TLPs might be related to the adaptation to winter climate in A. mongolicus. To investigate the roles of TLPs in winter adaptation, we first analyzed the expression pattern of TLP genes in A. mongolicus and then focused on the biological function and regulation pathway of AmTLP25 gene. Several TLP genes, including AmTLP25, were upregulated during winter and in response to both cold and osmotic stress. Overexpression of the AmTLP25 gene led to an increased tolerance of transgenic Arabidopsis to freezing and osmotic stress. Furthermore, the elevated AmWRKY14 transcription factor during winter activated AmTLP25 gene expression by specifically binding to its promoter. It is speculated that the AmWRKY14 – AmTLP25 module contributes to the adaptation to temperate winter climate in A. mongolicus. Our research advances the current understanding of the biological function and regulatory pathway of TLP genes and provides valuable information for understanding the molecular mechanism of temperate evergreen broad‐leaved plants adapting to winter climate.
Response of submarine braided channels to varying inflow hydrographs: Geomorphic experiments and stratigraphic implications
Authors: Lai Steven Y. J. , Liu Hsin‐Yun , Foreman Brady Z. , Limaye Ajay B. 
Published: September 2024

AbstractSubmarine channels on the seafloor experience a range of turbidity current‐flood hydrographs. The characteristics of the flood hydrograph influence channel dimensions, the number of channel threads (i.e. braiding intensity) and the depositional properties of sandbars within the channels. However, the morphodynamic response of submarine braided channels to different hydrograph patterns is poorly understood. In this study, six geomorphic experiments are used to test how varied flood hydrographs affect the braiding intensity and sandbar geometry within submarine channels. Three experiments represent the control group with constant density‐current inflow, and three experiments use different time‐varying hydrograph patterns. The experiments show that, as the inflow‐to‐sediment discharge ratio increases, multiple small channels coalesce into a few main channels, causing a decrease in active braiding intensity defined by the channel threads with flow. When the flow returns to the initial low flow rate, active braiding intensity increases again. These observations show that the inflow‐to‐sediment discharge ratio determines the value of active braiding intensity. Additionally, active braiding intensity is directly proportional to dimensionless sediment‐stream power and dimensionless stream power, in agreement with previously established trends for both submarine and fluvial braided channels. In contrast to active braiding intensity changes, the measured sandbar aspect ratio and compactness ratio (i.e. ratio of planform area to perimeter) of submarine braided channels is insensitive to changes in the hydrograph. However, when inflows reach peak discharge, the higher transport capacity erodes pre‐existing deposits, forming noticeable erosional surfaces, and subsequently depositing thicker sandbars during that stage. These observations imply that sandbar planform morphology is largely insensitive to hydrograph, but hydrograph variability induces a greater variability in the resultant stratigraphic packages. The experimental results predict that field‐scale stratigraphy should be dominated by strata deposited during intervals of high flow, but that these strata will also exhibit reduced lateral continuity compared to formation scenarios with constant discharge.
Comparative Religious Studies: A Pedagogy for Troubled Times?
Authors: Jensen Christopher , Vargas‐O'Bryan Ivette 
Published: September 2024

ABSTRACTIn this article, the authors explore their motivations for conceiving of and assembling the current issue on comparative religious studies as a viable pedagogical orientation for our troubled times. Situated in both the history of comparison as a method and scholarship on effective pedagogy, it argues that disciplined, scholarly, sound comparative thinking (whether at the level of textbook authorship, course design, or selection of classroom activities) can help us to respond to our students dynamically, reflexively, and, most importantly, humanely.
Becoming cultural and disability activists who challenge dominant narratives: The parallax effect of survivors of chemical warfare in Iran
Authors: Bajoghli Narges 
Published: September 2024

AbstractThis article discusses how Faye Ginsburg's work on ethnographic filmmaking, cultural activism, disability studies, and the parallax effect inspired me to collaborate over two decades with survivors of chemical warfare in Iran and Iraq. In order to continue to advocate for their needs for subsidized medical treatment, survivors of chemical weapons in Iran took up disability rights and carved out an important spaces as cultural activists to advocate for peace over war. Their creation of the Tehran Peace Museum and the many attendant media products it has produced, serve as an important parallax effect that has been instrumental in making critical dents into the extremely well‐funded state produced media about the war in Iran.