bluuebunny/crossref_metadata_2025_split huggingface dataset:
Keanekaragaman Makrozoobentos pada Ekosistem Air Tawar Lentik di Desa Gumulan Kecamatan Kesamben
Authors: Wulandari Anggun , Yuantina Yurin , Wardani Dian Kusuma , Sholihah Fatikhatun Nikmatus 
Published: September 2024

Penelitian ini bertujuan untuk mengetahui hasil identifikasi keanekaragaman makrozoobentos, hasil pengukuran faktor abiotik dan indeks keanekaragaman makrozoobentos pada ekosistem air tawar lentik di Desa Gumulan Kecamatan Kesamben. Penelitian dengan menggunakan metode survei lokasi dengan 3 stasiun di setiap ekosistem air tawar lentik. Penentuan titik pengambilan sampel ditentukan dengan menggunakan teknik purposive sampling pada 3 stasiun dengan pengulangan sebanyak 3 kali. Total spesies yang ditemukan pada ekosistem air tawar lentik yaitu 10 spesies diantaranya Pomacea speciosa, Assasin snail, Tarabia granifera, Macrobrachium formosense, Notonectidae, Tryonia clathrata, Lymnaeidae, Rabbit snail, Dytiscus marginalis, Gerris marginatus, yang dominan adalah Filum Mollusca yaitu spesies Tarebia granifera. Faktor abiotik pada ekosistem air tawar lentik pada stasiun 1 suhu rata-rata 29,5ºC dan rata-rata pH 7,5, stasiun 2 suhu rata-rata 28,4ºC dan rata-rata pH 7,1 dan stasiun 3 suhu rata-rata 29,3ºC dan rata-rata pH 7,8. Indeks keanekaragaman (H’) makrozoobentos pada ekosistem air tawar lentik berdasarkan rumus Shannon Weinner hasil dari stasiun 1 yaitu 1,47 (sedang), hasil stasiun 2 yaitu 1,56 (sedang) dan hasil stasiun 3 yaitu 1,01 (sedang).
أثر تمرينات مهارية في تطوير بعض المهارات الهجومية المنتهية بالتصويب السلمي للاعبي كرة السلة تحت18 سنة
Authors: Asst. Lect. Mustafa Fadel Khader  
Published: September 2024

تمثلت أهمية البحث في اعداد تمرينات مهارية تكون مشابهة لمواقف اللعب ليتم من خلالها العمل على تطوير المهارات الهجومية المنتهية بالتصويب السلمي كون هذا النوع من التصويب يكون هو الأكثر استخداماً من قبل اللاعبين في المباريات اما مشكلة البحث فقد تبلورت في وجود ضعف لدى لاعبين كرة السلة ( تحت 18 سنة) في المهارات الهجومية المنتهية بالتصويب السلمي التي يحتاجها اللاعب للارتقاء بمستواه المهاري في كرة السلة وخاصة في المواقف التي يكون فيها اللاعبين المهاجمين مراقبين بشكل جيد من قبل اللاعبين المدافعين مما يؤدي الى صعوبة انهاء الهجمات بدقة مالم يكن لدى اللاعبين المهاجمين المستوى المهاري الذي يؤهلهم للإنهاء الصحيح لهذا النوع من التصويب، وخصوصاً فئة الشباب التي تكون فيها خصوصية دقيقة كون اللاعب في مرحلة مهمة للانتقال الى مرحلة المتقدمين.
وهدف البحث الى:
1- إعداد تمرينات مهارية في تطوير بعض المهارات الهجومية المنتهية بالتصويب السلمي للآعبي كرة السلة (تحت 18سنة).
2- التعرف على تأثير التمرينات المهارية في تطوير بعض المهارات الهجومية المنتهية بالتصويب السلمي للآعبي كرة السلة (تحت 18سنة).
وفرض البحث هو:- -للتمرينات المهارية أثراً في تطوير المهارات الهجومية المنتهية بالتصويب السلمي للآعبين (تحت 18 سنة).
أما أهم الاستنتاجات:
-فاعلية التمرينات المهارية المستخدمة في الدراسة في تطوير المهارات الهجومية المنتهية بالتصويب السلمي.
التوصيات:
1-الإهتمام بتدريب اللاعبين على المهارات الهجومية المنتهية بالتصويب السلمي بمواقف تدريبية تكون مشابهة لمواقف اللعب في المباريات.
A nomogram model for early recurrence of HBV-related hepatocellular carcinomas after radical hepatectomy
Authors: Zhu Yu , Wang Ai-Dong , Gu Ling-Ling , Dai Qi-Qiang , Zheng Guo-Qun , Chen Ting , Wu Chun-Long , Jia Wei-Dong , Zhang Fa-Biao 
Published: September 2024

BackgroundTo identify the risk factors and construct a predictive model for early recurrence of hepatitis B virus(HBV-)- related hepatocellular carcinomas(HCCs) after radical resection.Data and methodsA total of 465 HBV-related HCC patients underwent radical resections between January 1, 2012 and August 31, 2018.Their data were collected through the inpatient information management system of the First Affiliated Hospital of University of Science and Technology of China. Survival and subgroup analyses of early recurrence among male and female patients were performed using Kaplan-Meier curves. The independent risk factors associated with early postoperative tumor recurrence were analyzed using multivariate Cox proportional hazards regression model. Based on these independent risk factors, a risk function model for early recurrence was fitted, and a column chart for the prediction model was drawn for internal and external validation.ResultsA total of 181 patients developed early recurrences, including 156 males and 25 females. There was no difference in the early recurrence rates between males and females. Tumor diameters&gt;5cm, microvascular invasion and albumin level&lt;35 g/L were independent risk factors for early recurrence. A nomogram for the early recurrence prediction model was drawn; the areas under the curve for the model and for external verification were 0.638 and 0.655, respectively.ConclusionTumor diameter&gt;5 cm, microvascular invasion, and albumin level&lt;35 g/L were independent risk factors for early recurrence. The prediction model based on three clinical indicators could predict early recurrence, with good discrimination, calibration, and extrapolation.
A PRDM16-driven signal regulates body composition in testosterone-treated hypogonadal men
Authors: Bathina Siresha , Colleluori Georgia , Villareal Dennis T. , Aguirre Lina , Chen Rui , Armamento-Villareal Reina 
Published: September 2024

BackgroundTestosterone (T) therapy increases lean mass and reduces total body and truncal fat mass in hypogonadal men. However, the underlying molecular mechanisms for the reciprocal changes in fat and lean mass in humans are not entirely clear.MethodsSecondary analysis of specimens obtained from a single-arm, open-label clinical trial on pharmacogenetics of response to T therapy in men with late-onset hypogonadism, conducted between 2011 and 2016 involving 105 men (40-74 years old), who were given intramuscular T cypionate 200 mg every 2 weeks for 18 months. Subcutaneous fat (SCF), peripheral blood mononuclear cells (PBMC) and serum were obtained from the participants at different time points of the study. We measured transcription factors for adipogenesis and myogenesis in the SCF, and PBMC, respectively, by real-time quantitative PCR at baseline and 6 months. Serum levels of FOLLISTATIN, PAX7, MYOSTATIN, ADIPSIN, and PRDM16 were measured by ELISA.ResultsAs expected, there was a significant increase in T and estradiol levels after 6 months of T therapy. There was also a reduction in fat mass and an increase in lean mass after 6 months of T therapy. Gene-protein studies showed a significant reduction in the expression of the adipogenic markers PPARγ in SCF and ADIPSIN levels in the serum, together with a concomitant significant increase in the expression of myogenic markers, MYOD in PBMC and PAX7 and FOLLISTATIN levels in the serum after 6 months of T therapy compared to baseline. Interestingly, there was a significant increase in the adipo-myogenic switch, PRDM16, expression in SCF and PBMC, and in circulating protein levels in the serum after 6 months of T therapy, which is likely from increased estradiol.ConclusionOur study supports that molecular shift from the adipogenic to the myogenic pathway in men with hypogonadism treated with T could be mediated directly or indirectly by enhanced PRDM16 activity, in turn a result from increased estradiol level. This might have led to the reduction in body fat and increase in lean mass commonly seen in hypogonadal men treated with T.
Analysis of risk factors for papillary thyroid carcinoma and the association with thyroid function indicators
Authors: Liu Jianning , Feng Zhuoying , Gao Ru , Liu Peng , Meng Fangang , Fan Lijun , Liu Lixiang , Du Yang 
Published: September 2024

ObjectiveThis study aims to analyze the relationship between papillary thyroid carcinoma (PTC) and various factors.MethodsThe study involved two groups—PTC patients and non-PTC controls. We utilized binary logistic regression and Least Absolute Shrinkage and Selection Operator (Lasso) regression for variable selection and risk factor analysis. Correlation analysis was performed using Spearman’s rank correlation. The diagnostic value of thyroid stimulating hormone (TSH) levels for PTC was assessed using Receiver Operating Characteristic (ROC) curves.ResultsPTC patients exhibited higher body mass index (BMI) (23.71 vs. 22.66, p&lt;0.05) and TSH levels (3.38 vs. 1.59, p&lt;0.05). Urinary iodine concentration (UIC) was an independent predictor of PTC (OR=1.005, p&lt;0.05). The optimal TSH threshold for PTC diagnosis was 2.4 mIU/L [The Area Under the Curve (AUC)=67.3%, specificity=71.4%, sensitivity=70.1%]. TSH levels positively correlated with BMI (r=0.593, p&lt;0.05) and UIC (r=0.737, p&lt;0.05).ConclusionsUIC may be an independent predictor of PTC, and TSH levels have some diagnostic value for identifying PTC.
Paraventricular oxytocin neurons impact energy intake and expenditure: projections to the bed nucleus of the stria terminalis reduce sucrose consumption
Authors: Worth Amy A. , Feetham Claire H. , Morrissey Nicole A. , Luckman Simon M. 
Published: September 2024

BackgroundThe part played by oxytocin and oxytocin neurons in the regulation of food intake is controversial. There is much pharmacological data to support a role for oxytocin notably in regulating sugar consumption, however, several recent experiments have questioned the importance of oxytocin neurons themselves.MethodsHere we use a combination of histological and chemogenetic techniques to investigate the selective activation or inhibition of oxytocin neurons in the hypothalamic paraventricular nucleus (OxtPVH). We then identify a pathway from OxtPVH neurons to the bed nucleus of the stria terminalis using the cell-selective expression of channel rhodopsin.ResultsOxtPVH neurons increase their expression of cFos after both physiological (fast-induced re-feeding or oral lipid) and pharmacological (systemic administration of cholecystokinin or lithium chloride) anorectic signals. Chemogenetic activation of OxtPVH neurons is sufficient to decrease free-feeding in OxtCre:hM3Dq mice, while inhibition in OxtCre:hM4Di mice attenuates the response to administration of cholecystokinin. Activation of OxtPVH neurons also increases energy expenditure and core-body temperature, without a significant effect on locomotor activity. Finally, the selective, optogenetic stimulation of a pathway from OxtPVH neurons to the bed nucleus of the stria terminalis reduces the consumption of sucrose.ConclusionOur results support a role for oxytocin neurons in the regulation of whole-body metabolism, including a modulatory action on food intake and energy expenditure. Furthermore, we demonstrate that the pathway from OxtPVH neurons to the bed nucleus of the stria terminalis can regulate sugar consumption.
Different intensities of aerobic training for patients with type 2 diabetes mellitus and knee osteoarthritis: a randomized controlled trial
Authors: Su Chi , Huang Lihua , Tu Shaochen , Lu Shengdi 
Published: September 2024

ObjectiveThe purpose of this study was to compare different intensities of aerobic exercise for patients with knee osteoarthritis (KOA) and type 2 diabetes mellitus (T2DM) in terms of glycemic control, pain relief, and functional outcomes.MethodsA prospective randomized open-label parallel multicenter clinical trial conducted at two hospitals in Shanghai and Sichuan that included 228 patients with type 2 diabetes mellitus (T2DM) and knee osteoarthritis (KOA). Enrollment occurred between January 2021 and February 2023, and follow-up was completed in September 2023. Participants were randomized to threshold training/high-intensive stationary cycling training (n=76), intensive endurance/moderate-intensive stationary cycling training (n=77), and regular rehabilitation programs (n=75). The primary outcome at the 6-month follow-up was the HbA1c level. Key secondary outcomes included the Knee Injury and Osteoarthritis Outcome Score (KOOS) subscale of pain and quality of life.ResultsOf 228 patients, 212 (93%) completed the trial. The mean adjusted (sex, baseline BMI, and baseline outcome measures) HbA1c level at the 6-month follow-up decreased significantly in the high-intensive training group compared with other groups (high-intensity group vs. control group; difference, 0.51%, 95% confidence interval, 0.05% to 1.15%). Mean KOOS subscales of pain and quality of life were statistically significantly different between the control group and moderate-intensity or high-intensity groups, but no statistical differences were noted between the different intensities of aerobic exercise. Patients in all groups achieved a greater reduction in BMI but no significant differences were observed between groups.ConclusionIn KOA and T2DM patients, high-intensity stationary cycling can significantly improve glycemic control compared with moderate-intensity and regular rehabilitation programs. However, high-intensity stationary cycling does not exert a superior effect on pain relief and functional improvement for KOA compared with moderate-intensity and regular rehabilitation programs.
Effects of dietary supplementation with medicinal plant mixtures and immunostimulants on the immune response, antioxidant capacity, and hepatopancreatic health of Chinese mitten crab (Eriocheir sinensis)
Authors: Wang Anran , Xu Jie , Zhang Xin , Liu Xuran , Li Mengge , Dong Xiaojing , Miao Shuyan 
Published: September 2024

IntroductionThis study aimed to evaluate the efficiency of tea polyphenols (TP) and medicinal plant mixtures (Astragalus membranaceus + Lonicera japonica, Rheum officinale Bail + Scutellaria baicalensis + Platycladus orientalis) combined with astaxanthin (AST), benzoic acid (BA), and yeast complex on the health status of Eriocheir sinensis.MethodA total of 630 crabs (male crabs: 41.51 ± 1.63 g; female crabs: 47.27 ± 0.79 g) were randomly distributed into seven groups with three replicates (male: female, 1:1). These crabs were fed as follows for 8 weeks: basal diet (M1), M2 (M1 + 100 mg/kg TP), M3 (M1 + 2.0 g/kg A. membranaceus + 20 g/kg L. japonica), M4 (M1 + 2.5 g/kg R. officinale Bail + 1.5 g/kg S. baicalensis + 1.0 g/kg P. orientalis), and M5, M6, M7 (M2, M3 and M4 with 600 mg/kg AST +1.0 g/kg BA + 20 mg/kg yeast complex added, respectively).Results and discussionThe results showed that the activities of acid phosphatase (ACP), alkaline phosphatase (AKP), and lysosome (LZM) in the hemolymph were significantly increased in M5, M6, and M7 (P &lt; 0.05), and the highest phagocytosis index (PI) and LZM activity were observed in M7 of female crabs. Moreover, the antioxidant indicators superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx), and catalase (CAT) of hepatopancreas were also significantly improved in M5, M6, and M7 (P &lt; 0.05), while the malondialdehyde (MDA) contents showed an opposite trend. Furthermore, a morphological examination also showed the improved histological structure of hepatopancreas in M7, especially as seen in the clear lumens, no vacuolation, and integrity of the basal membrane of the hepatopancreatic tubule. Taken together, these results suggested that 2.5 g/kg R. officinale Bail, 1.5 g/kg S. baicalensis, and 1.0 g/kg P. orientalis in combination with 600 mg/kg AST, 1.0 g/kg BA, and 20 mg/kg yeast complex could improve the non-specific immunity, antioxidant capacity, and hepatopancreatic health of E. sinensis.
Case report: Granulocyte-macrophage colony-stimulating factor sargramostim did not rescue the neutrophil phenotype in two patients with JAGN1-mutant severe congenital neutropenia
Authors: Farmand Susan , Aydin Susanne Eva , Wustrau Katharina , Böhm Svea , Ayuk Francis , Escherich Gabriele , Skokowa Julia , Müller Ingo , Lehmberg Kai 
Published: September 2024

BackgroundHomozygous or compound heterozygous mutations in JAGN1 cause severe congenital neutropenia. JAGN1-mutant patients present with severe early-onset bacterial infections and most have been described as low-responders to recombinant granulocyte colony-stimulating factor (G-CSF) therapy. In a murine, hematopoietic JAGN1 knockout model, which displays susceptibility to Candida albicans infection in the absence of neutropenia, treatment with granulocyte-macrophage-CSF (GM-CSF) was able to restore the functional defect of neutrophils.PatientsWe present two unrelated patients with biallelic JAGN1 mutations, who were both treated with subcutaneous GM-CSF (sargramostim) after treatment failure to G-CSF. The first patient was an 18-year-old pregnant woman who received GM-CSF at 12 weeks of gestation up to a dose of 10 µg/kg/d for 7 days. The second patient was a 5-month-old girl who received GM-CSF for a total of 9 days at a dose of up to 20 µg/kg/d. GM-CSF did not increase neutrophil counts in our patients. Treatment was stopped when neutrophil numbers declined further, no beneficial effect was noticed, and patients presented with infections. No adverse effects were observed in either patient and the fetus. Both patients ultimately underwent successful hematopoietic stem cell transplantation.DiscussionBoth patients showed a high recurrence rate of severe infections on G-CSF treatment. GM-CSF therapy did not ameliorate the clinical phenotype, in contrast to the improvement of neutrophil function observed in the JAGN1 mouse model. No major additional extra-hematopoietic manifestations were evident in our patients.ConclusionIn two unrelated patients, GM-CSF did not have any beneficial effect on neutrophil counts. Patients with JAGN1-mutant SCN with reduced G-CSF responsiveness and elevated infection rate should be evaluated early for stem cell transplantation.
The causal relationship between immune cells and diabetic retinopathy: a Mendelian randomization study
Authors: Ye Yunyan , Dai Lei , Gu Hong , Yang Lan , Xu Zhangxing , Li Zhiguo 
Published: September 2024

PurposeThis article explored the causal relationship between immune cells and diabetic retinopathy (DR) using single nucleotide polymorphisms (SNPs) as an instrumental variable and Mendelian randomization (MR).MethodsStatistical data were collected from a publicly available genome-wide association study (GWAS), and SNPs that were significantly associated with immune cells were used as instrumental variables (IVs). Inverse variance weighted (IVW) and MR−Egger regression were used for MR analysis. A sensitivity analysis was used to test the heterogeneity, horizontal pleiotropy, and stability of the results.ResultsWe investigated the causal relationship between 731 immune cells and DR risk. All the GWAS data were obtained from European populations and from men and women. The IVW analysis revealed that HLA DR on CD14+ CD16- monocytes, HLA DR on CD14+ monocytes, HLA DR on CD33-HLA DR+, HLA DR on CD33+ HLA DR+ CD14- on CD33+ HLA DR+ CD14dim, and HLA DR on myeloid dendritic cells may increase the risk of DR (P&lt;0.05). HLA DR to CD14-CD16- cells, the monocytic myeloid-derived suppressor cell absolute count, the SSC-A count of CD4+ T cells, and terminally differentiated CD4+ T cells may be protective factors against DR (P&lt;0.05). The sensitivity analysis indicated no heterogeneity or pleiotropy among the selected SNPs. Furthermore, gene annotation of the SNPs revealed significant associations with 10 genes related to the risk of developing PDR and potential connections with 12 other genes related to PDR.ConclusionMonocytes and T cells may serve as new biomarkers or therapeutic targets, leading to the development of new treatment options for managing DR.
Proliferation capability of natural killer cells upon cytokines stimulation correlated negatively with serum lactate dehydrogenase level in coronary artery disease patients
Authors: Guo Xuemin , Xiao Ting , Lin Li , Gao Qianqian , Lai Bifa , Liu Xianhui , Zhong Zhixiong 
Published: September 2024

BackgroundNatural killer (NK) cells are proposed to participate in coronary artery disease (CAD) development. However, little is known about how CAD patients’ NK cells respond to different stimulatory factors in terms of proliferation capability.Methods and resultsTwenty-nine CAD patients’ peripheral blood NK cells were isolated and individually treated with IL-2, IL-12, IL-15, IL-18, IL-21, cortisone acetate, hydrocortisone, or ascorbic acid for 36 hours, followed by cell cycle analysis using flow cytometry. The ratio of S and G2/M phase cell number to total cell number was defined as a proliferation index (PrI) and used for proliferative capability indication. The results showed that these eight factors resulted in different life cycle changes in the 29 NK cell samples. Remarkably, 28 out of 29 NK cell samples showed an obvious increase in PrI upon ascorbic acid treatment. The serum lactate dehydrogenase (LDH) level of the 29 CAD patients was measured. The results showed a negative correlation between serum LDH level and the CAD patients’ NK cell PrI upon stimulation of interleukins, but not the non-interleukin stimulators. Consistently, a retrospective analysis of 46 CAD patients and 32 healthy donors showed that the circulating NK cell number negatively correlated with the serum LDH level in CAD patients. Unexpectedly, addition of LDH to NK cells significantly enhanced the production of IFN-γ, IL-10 and TNF-α, suggesting a strong regulatory role on NK cell’s function.ConclusionAscorbic acid could promote the proliferation of the CAD patients’ NK cells; LDH serum level may function as an indicator for NK cell proliferation capability and an immune-regulatory factor.
Booster vaccination using bivalent DS-5670a/b is safe and immunogenic against SARS-CoV-2 variants in children aged 5–11 years: a phase 2/3, randomized, active-controlled study
Authors: Suzuki Rino , Suda Miharu , Ishida Katsuyasu , Furihata Kei , Ota Aisaku , Takahashi Kaori , Sakakibara Sachiko , Nakayama Tetsuo , Takeshita Fumihiko 
Published: September 2024

BackgroundDS-5670 is a messenger ribonucleic acid (mRNA) vaccine platform targeting the receptor-binding domain (RBD) of the spike protein derived from severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Booster vaccination against coronavirus disease 2019 (COVID-19) with monovalent DS-5670a (incorporating mRNA encoding the RBD from the original SARS-CoV-2 strain) or bivalent DS-5670a/b (original and omicron BA.4-5 RBD antigens) is effective and safe in adults. Data from a phase 2/3 active-controlled, non-inferiority, pediatric study evaluating a third booster dose of DS-5670a/b are reported here.MethodsChildren aged 5–11 years who had completed the two-dose primary vaccination series with monovalent BNT162b2 (original strain) at least 3 months prior to enrolment were randomly assigned to receive DS-5670a/b (20 µg of mRNA) or bivalent BNT1 62b2 (original/omicron BA.4-5; 10 µg of mRNA) on Day 1. The primary efficacy endpoint was blood neutralization geometric mean titer (GMT) against SARS-CoV-2 (omicron variant BA.5.2.1) and immune response rate (≥ 4-fold increase in post-vaccination circulating anti-SARS-CoV-2 neutralizing activity) on Day 29.ResultsAmong evaluable participants (DS-5670a/b, n = 74; bivalent BNT162b2, n = 75), the adjusted GMT ratio of DS-5670a/b to bivalent BNT162b2 on Day 29 was 1.636 (95% CI, 1.221, 2.190). Immune response rates were ≥ 89% with both study vaccines; adjusted difference 2.6% (95% CI, –7.8, 13.8). The prespecified non-inferiority margins were exceeded, and the study met the primary endpoint. DS-5670a/b also demonstrated broad neutralization activity across recent omicron sublineages and no cases of COVID-19 between Days 8–29 post-administration were reported. There were no novel safety concerns in the pediatric population at data cut-off.ConclusionsBivalent DS-5670a/b was non-inferior to bivalent BNT162b2 in terms of immunogenicity, and had a manageable safety profile, when administered as a heterologous booster in children aged 5–11 years.Clinical trial registrationhttps://jrct.niph.go.jp/, identifier jRCT2031220665