bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequence of Staphylococcus epidermidis 1457
Authors: Galac Madeline R. , Stam Jason , Maybank Rosslyn , Hinkle Mary , Mack Dietrich , Rohde Holger , Roth Amanda L. , Fey Paul D. 
Published: June 2017

ABSTRACT

Staphylococcus epidermidis
1457 is a frequently utilized strain that is amenable to genetic manipulation and has been widely used for biofilm-related research. We report here the whole-genome sequence of this strain, which encodes 2,277 protein-coding genes and 81 RNAs within its 2.4-Mb genome and plasmid.
Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase
Authors: Smith Paul , Ho C. Kiong , Takagi Yuko , Djaballah Hakim , Shuman Stewart 
Published: June 2017

ABSTRACT

Eukaryal taxa differ with respect to the structure and mechanism of the RNA triphosphatase (RTPase) component of the mRNA capping apparatus. Protozoa, fungi, and certain DNA viruses have a metal-dependent RTPase that belongs to the triphosphate tunnel metalloenzyme (TTM) superfamily. Because the structures, active sites, and chemical mechanisms of the TTM-type RTPases differ from those of mammalian RTPases, the TTM RTPases are potential targets for antiprotozoal, antifungal, and antiviral drug discovery. Here, we employed RNA interference (RNAi) knockdown methods to show that
Trypanosoma brucei
RTPase Cet1 (TbCet1) is necessary for proliferation of procyclic cells in culture. We then conducted a high-throughput biochemical screen for small-molecule inhibitors of the phosphohydrolase activity of TbCet1. We identified several classes of chemicals—including chlorogenic acids, phenolic glycopyranosides, flavonoids, and other phenolics—that inhibit TbCet1 with nanomolar to low-micromolar 50% inhibitory concentrations (IC
50
s). We confirmed the activity of these compounds, and tested various analogs thereof, by direct manual assays of TbCet1 phosphohydrolase activity. The most potent nanomolar inhibitors included tetracaffeoylquinic acid, 5-galloylgalloylquinic acid, pentagalloylglucose, rosmarinic acid, and miquelianin. TbCet1 inhibitors were less active (or inactive) against the orthologous TTM-type RTPases of mimivirus, baculovirus, and budding yeast (
Saccharomyces cerevisiae
). Our results affirm that a TTM RTPase is subject to potent inhibition by small molecules, with the caveat that parallel screens against TTM RTPases from multiple different pathogens may be required to fully probe the chemical space of TTM inhibition.

IMPORTANCE
The stark differences between the structure and mechanism of the RNA triphosphatase (RTPase) component of the mRNA capping apparatus in pathogenic protozoa, fungi, and viruses and those of their metazoan hosts highlight RTPase as a target for anti-infective drug discovery. Protozoan, fungal, and DNA virus RTPases belong to the triphosphate tunnel metalloenzyme family. This study shows that a protozoan RTPase, TbCet1 from
Trypanosoma brucei
, is essential for growth of the parasite in culture and identifies, via
in vitro
screening of chemical libraries, several classes of potent small-molecule inhibitors of TbCet1 phosphohydrolase activity.
Two-diode behavior in metal-ferroelectric-semiconductor structures with bismuth titanate interfacial layer
Authors: Durmuş Perihan , Altindal Şemsettin 
Published: June 2017

In this study, electrical parameters of the Al/Bi4Ti3O[Formula: see text]/p-Si metal-ferroelectric-semiconductor (MFS) structure and their temperature dependence were investigated using current–voltage (I–V) data measured between 120 K and 300 K. Semi-logarithmic I–V plots of the structure revealed that fabricated structure presents two-diode behavior that leads to two sets of ideality factor, reverse saturation current and zero-bias barrier height (BH) values. Obtained results of these parameters suggest that current conduction mechanism (CCM) deviates strongly from thermionic emission theory particularly at low temperatures. High values of interface states and nkT/q[Formula: see text]−[Formula: see text]kT/q plot supported the idea of deviation from thermionic emission. In addition, [Formula: see text](I)[Formula: see text]−[Formula: see text][Formula: see text](V) plots suggested that CCM varies from one bias region to another and depends on temperature as well. Series resistance values were calculated using Ohm’s law and Cheungs’ functions, and they decreased drastically with increasing temperature.
Catchment-based gold prospectivity analysis combining geochemical, geophysical and geological data across northern Australia
Authors: Cracknell M. J. , de Caritat P. 
Published: June 2017

The results of a pilot study into the application of an unsupervised clustering approach to the analysis of catchment-based National Geochemical Survey of Australia (NGSA) geochemical data combined with geophysical and geological data across northern Australia are documented. NGSA Mobile Metal Ion® (MMI) element concentrations and first and second order statistical summaries across catchments of geophysical data and geological data are integrated and analysed using Self-Organizing Maps (SOM). Input features that contribute significantly to the separation of catchment clusters are objectively identified and assessed.
A case study of the application of SOM for assessing the spatial relationships between Au mines and mineral occurrences in catchment clusters is presented. Catchments with high mean Au code-vector concentrations are found downstream of areas known to host Au mineralization. This knowledge is used to identify upstream catchments exhibiting geophysical and geological features that indicate likely Au mineralization. The approach documented here suggests that catchment-based geochemical data and summaries of geophysical and geological data can be combined to highlight areas that potentially host previously unrecognised Au mineralization.
Tumor-targeted Nanoparticle Delivery of HuR siRNA Inhibits Lung Tumor Growth In Vitro and In Vivo By Disrupting the Oncogenic Activity of the RNA-binding Protein HuR
Authors: Muralidharan Ranganayaki , Babu Anish , Amreddy Narsireddy , Srivastava Akhil , Chen Allshine , Zhao Yan Daniel , Kompella Uday B. , Munshi Anupama , Ramesh Rajagopal 
Published: June 2017

Abstract
Selective downregulation of the human antigen R (HuR) protein by siRNA may provide a powerful approach for treating lung cancer. To this end, we investigated the efficacy of transferrin receptor-targeted liposomal nanoparticle-based HuR siRNA (HuR-TfNP) therapy and compared with control siRNA (C)-TfNP therapy both, in vitro and in vivo using lung cancer models. In vitro studies showed HuR-TfNP, but not C-TfNP, efficiently downregulated HuR and HuR-regulated proteins in A549, and HCC827 lung cancer cells, resulting in reduced cell viability, inhibition of cell migration and invasion, and induction of G1 cell-cycle arrest culminating in apoptosis. However, HuR-TfNP activity in normal MRC-9 lung fibroblasts was negligible. In vivo biodistribution study demonstrated that fluorescently labeled HuR-siRNA or ICG dye–loaded TfNP localized in tumor tissues. Efficacy studies showed intratumoral or intravenous administration of HuR-TfNP significantly inhibited A549 (&gt;55% inhibition) and HCC827 (&gt;45% inhibition) subcutaneous tumor growth compared with C-TfNP. Furthermore, HuR-TfNP treatment reduced HuR, Ki67, and CD31 expression and increased caspase-9 and PARP cleavage and TUNEL-positive staining indicative of apoptotic cell death in tumor tissues compared with C-TfNP treatment. The antitumor activity of HuR-TfNP was also observed in an A549-luc lung metastatic model, as significantly fewer tumor nodules (9.5 ± 3.1; P &lt; 0.001; 88% inhibition) were observed in HuR-TfNP–treated group compared with the C-TfNP–treated group (77.7 ± 20.1). Significant reduction in HuR, Ki67, and CD31 expression was also observed in the tumor tissues of HuR-TfNP-treatment compared with C-TfNP treatment. Our findings highlight HuR-TfNP as a promising nanotherapeutic system for lung cancer treatment. Mol Cancer Ther; 16(8); 1470–86. ©2017 AACR.
Transverse microhardness of artificial teeth
Authors: Tamaki Regina , Iegami Carolina Mayumi , Uehara Priscila Nakasone , Furuyama Ricardo Jun , Ballester Rafael Yague 
Published: June 2017

Objetivo: A dureza é tida como indicador de várias propriedades mecânicas de dentes artificiais, e relacionada a resistência ao desgaste. O objetivo foi mapear a microdureza de dentes artificiais de 9 marcas em função da profundidade. Método: foi medida a microdureza Knoop de segundos molares cortados num plano sagital em intervalos de 200 µm a partir da profundidade de 100 µm até 4700 µm, das marcas: Premium (Pr), Orthosit (Or), SR Postaris DCL (Po), Biotone (Bi), Artiplus IPN (Ar), VITA MFT (Vi), Natusdent (Na), Trilux (Tr) and Biolux (Bx). A ANOVA de medidas repetidas e o teste de Tukey (5%) analisaram os resultados. Resultados: A marca SR Orthosit PE apresentou a maior dureza (até a profundidade de 3,1 mm foi da ordem de 30 N/mm2), significativamente maior que todas as outras medidas realizadas (da ordem de 20 N/mm2). Conclusões: A dureza Knoop não evidenciou diferenças entre camadas para oito das nove marcas estudadas. Foi constatada diferente dureza entre a superfície e a região cervical para a marca SR Orthosit PE.
Ameloblastic fibroma: a case report
Authors: Carvalho Eloisa Muller de , Horikawa Fernando Kendi , Guimaraes Letícia , Viveiros Stephanie Kenig , Lemos Celso Augusto , Araujo Juliane Piragine 
Published: June 2017

Ameloblastic fibroma is a rare benign odontogenic tumor in which both the epithelial and ectomesenchymal components are neoplastic. A 24-year-old male patient was referred to the Stomatology Department presenting with difficulty to chew and swelling in the right posterior region of the mandible. The panoramic radiograph showed a well-circumscribed, unilocular radiolucent lesion with partially radiopaque borders involving first and second unerupted molars. Computed tomography imaging presented a hypodense image with well‑delimited isodense content, bulging cortical bones and absence of rupture. The patient underwent an incisional biopsy. Microscopically, the lesion was composed of many mesenchymal tissue cells in strand form, arranged in cords, islands and nests of odontogenic epithelium; the diagnostic was ameloblastic fibroma. The patient was referred to the hospital for enucleation and curettage of the lesion and extraction of the associated teeth. After 8 months of follow-up, no recurrence was observed. This case emphasizes the importance of differential diagnosis, anatomopathological exam, and both clinical and imaging follow-up, since tumors of this type can recur and progress to malignancy.
Three new Macropsini (Hemiptera: Cicadellidae) leafhopper species from Australia
Authors: YANG LIYUAN , DIETRICH CHRISTOPHER H. , ZHANG YALIN 
Published: June 2017

Three new species, Macropsella recta, Toropsis minuspina and Varicopsella apecurvata spp. nov. are described and illustrated from Australia. Leafhoppers of the subfamily Macropsinae are found abundantly in the Holarctic, Oriental and Australian regions (Linnavuori, 1978) and have been collected from around the world, except for Antarctica, Oceania and South America (Hamilton, 1980). Both Hamilton (1980) and Evans (1966) suggested that there were likely a myriad of uncollected and undescribed species in Australia alone and Evans (1971) commented that the Macropsinae are possibly more abundant in Australia than anywhere else in the world. Day and Fletcher (1994) listed 45 macropsine species in eight genera and mentioned that the Australian fauna needs “a thorough examination to establish the generic affinities of the species…”. In her unpublished doctoral dissertation, Semeraro (2014) recently completed a revision and phylogeny of the Australian fauna, documenting an additional 50 undescribed species and proposing changes to the generic classification, but this work has not yet been published. The new Australian species described herein, representing three genera, one not previously recorded in Australia, were not included in Semeraro’s (2014) dissertation.Study of samples recently collected from Australia revealed the presence of 3 new species, representing the genera Macropsella Hamilton, Toropsis Hamilton and Varicopsella Hamilton.Macropsella was established by Hamilton (1980) with Macropsis saidora Evans, 1971 as the type species. Five Macropsis species describeded by Evans (1971), one Macropsis species described by Kirkaldy (1907) and new species Macropsella complicata Hamilton (1980) were previously included in this genus. The seven species of this genus are known only from New Guinea and Northern Australia. Members of this genus can be distinguished by their usually white spotted tegminal veins, tapered male pygofer and laterally directed short ventral pygofer spines.Toropsis was established by Hamilton (1980) with Oncopsis balli Kirkaldy, 1907 as the type species. Six Oncopsis species (Evans, 1935, 1941) and three Macropsis species (Evans, 1971; Metcalf, 1966) were transferred to this genus by Hamilton (1980). So far, ten species recorded in this genus, and all of these species are recorded only from Australia. Toropsis can be distinguished by the wide face, small and flat front, inflated pronotum, unarmed male pygofer and relatively small dorsal connectives.Varicopsella was established by Hamilton (1980) for seven species from the Oriental region, with Macropsis breakeyi Merino, 1936 as its type species. More recently, Li et al. (2014) added a new species and subgenus Varicopsella (Multispinulosa) hamiltoni from China, but this species appears to be incorrectly placed in Varicopsella. Yang et al. (2016) added V. odontoida from Thailand. This genus can be distinguished by the depressed head, fused lora and frontoclypeus, and the two-jointed dorsal connective of the male.
The Roles of Convection over the Western Maritime Continent and the Philippine Sea in Interannual Variability of Summer Rainfall over Southwest China
Authors: Jiang Xingwen , Shu Jianchuan , Wang Xin , Huang Xiaomei , Wu Qing 
Published: June 2017

Abstract
Floods and droughts hit southwest China (SWC) frequently, especially over the last decade. In this study, the dominant modes of summer rainfall anomalies over SWC on the interannual time scale and the possible causes are investigated. Interannual variability of the summer rainfall over SWC has two dominant modes. The first mode features rainfall increases over most of SWC except central Sichuan, and the second mode exhibits wet conditions in the north but dry conditions in the south. The suppressed convection over the Philippine Sea affects the first mode by inducing anomalous anticyclones over the western North Pacific and to the south of the Tibetan Plateau, which transport more water vapor to eastern Tibet and eastern SWC and hence favor above-normal rainfall there. The enhanced convection over the western Maritime Continent could generate similar atmospheric circulation anomalies associated with the suppressed convection over the Philippine Sea but with a northward shift, resulting in significant increases in rainfall over northeastern SWC but weak decreases in rainfall over southeastern SWC. As a result, the rainfall anomalies over SWC tend to be different between El Niño–Southern Oscillation decaying and developing phases because their different impacts on the convection over the Philippine Sea and the western Maritime Continent. Meanwhile, the sea surface temperature in the tropical southeastern Indian Ocean also plays an important role in variability of the rainfall over SWC because of its significant impact on the convection over the western Maritime Continent.
The Recognizability and Localizability of Auditory Alarms: Setting Global Medical Device Standards
Authors: Edworthy Judy , Reid Scott , McDougall Siné , Edworthy Jonathan , Hall Stephanie , Bennett Danielle , Khan James , Pye Ellen 
Published: June 2017

Objective Four sets of eight audible alarms matching the functions specified in IEC 60601-1-8 were designed using known principles from auditory cognition with the intention that they would be more recognizable and localizable than those currently specified in the standard. Background The audible alarms associated with IEC 60601-1-8, a global medical device standard, are known to be difficult to learn and retain, and there have been many calls to update them. There are known principles of design and cognition that might form the basis of more readily recognizable alarms. There is also scope for improvement in the localizability of the existing alarms. Method Four alternative sets of alarms matched to the functions specified in IEC 60601-1-8 were tested for recognizability and localizability and compared with the alarms currently specified in the standard. Results With a single exception, all prototype sets of alarms outperformed the current IEC set on both recognizability and localizability. Within the prototype sets, auditory icons were the most easily recognized, but the other sets, using word rhythms and simple acoustic metaphors, were also more easily recognized than the current alarms. With the exception of one set, all prototype sets were also easier to localize. Conclusion Known auditory cognition and perception principles were successfully applied to an existing audible alarm problem. Application This work constitutes the first (benchmarking) phase of replacing the alarms currently specified in the standard. The design principles used for each set demonstrate the relative ease with which different alarm types can be recognized and localized.
Radiation-induced esophageal strictures treated with fluoroscopic balloon dilation: clinical outcomes and factors influencing recurrence in 62 patients
Authors: Park Jung-Hoon , Kim Kun Yung , Song Ho-Young , Cho Young Chul , Kim Pyeong Hwa , Tsauo Jiaywei , Kim Min Tae , Jun Eun Jung , Jung Hwoon-Yong , Kim Sung-Bae , Kim Jong Hoon 
Published: June 2017

Background Balloon dilation is safe and effective for the treatment of radiation-induced esophageal stricture (RIES), with favorable short-term and mid-term outcomes; however, few reports of long-term outcomes exist. Few studies have evaluated factors associated with recurrence after balloon dilation. Purpose To evaluate the long-term outcome of balloon dilation in patients with RIES and to identify factors associated with stricture recurrence. Material and Methods The medical records of 62 consecutive patients who had undergone fluoroscopic balloon dilation for RIES at our institution between December 1998 and June 2016 were reviewed. Results One hundred and twenty balloon dilation sessions were performed in 62 patients (mean = 1.9 sessions per patient). Clinical success was achieved in 53 (86%) patients after single (n = 37) or multiple (n = 16) dilation sessions. Complications occurred in 27% of the dilation sessions. The primary patency rates at one, two, three, and five years were 60%, 56%, 52%, and 52%, respectively. Secondary patency rates at one, two, three, and five years were 87%, 85%, 85%, and 80%, respectively. Multivariate logistic regression analysis identified an interval from radiation therapy (RT) to stricture of ≥6 months (hazard ratio [HR] = 0.205; P < 0.001), strictures located at the cervical esophagus (HR = 5.846; P < 0.001), and stricture length of ≥2 cm (HR = 2.923; P = 0.006) as significant predictors of recurrence. Conclusion Despite the high incidence of ruptures and recurrences, fluoroscopic balloon dilation is valuable as an initial therapeutic option for patients with RIES.
Matrix Production Affects MRI Outcomes After Matrix-Associated Autologous Chondrocyte Transplantation in the Knee
Authors: Albrecht Christian , Reuter Carla-Antonia , Stelzeneder David , Zak Lukas , Tichy Brigitte , Nürnberger Sylvia , Boesmueller Sandra , Marlovits Stefan , Trattnig Siegfried , Hajdu Stefan , Aldrian Silke 
Published: June 2017

Background: Matrix-associated autologous chondrocyte transplantation (MACT) has been an effective therapy for large, full-thickness cartilage lesions for years. However, little is known about how graft maturation is affected by characteristics of transplanted chondrocytes. Purpose: To investigate the influence of gene expression of chondrocytes at the time of transplantation on MRI outcomes up to 2 years after MACT. Study Design: Case series; Level of evidence, 4. Methods: This study included 25 patients with 27 symptomatic traumatic defects of articular cartilage, who had undergone MACT in the knee. Postoperative MRI examinations were conducted at 3, 6, 12, and 24 months after surgery. Biochemical graft maturation was assessed by measuring T2 relaxation time values of the transplant and healthy native cartilage areas. The MOCART (magnetic resonance observation of cartilage repair tissue) score was used to evaluate the morphological quality of regeneration tissue. Gene expression (collagen type I, collagen type II, aggrecan, versican, and interleukin-1β) was determined by real-time polymerase chain reaction (PCR) in transplant residuals at the time point of transplantation and was correlated with MRI outcomes using Spearman’s rank correlation coefficient. A Friedman test with post hoc analysis (Wilcoxon signed rank test) conducted with a Bonferroni correction was applied to compare scores at different time points. Results: T2 relaxation time of regeneration tissue improved from a mean ± SD of 74.6 ± 20.1 milliseconds at 3 months to 47.9 ±13.3 milliseconds at 24 months ( P < .003). These values were similar to the T2 relaxation times of the native surrounding cartilage (50.9 ± 15 ms). The calculated T2 index (ratio of regeneration tissue to native cartilage) improved from 1.63 ± 0.76 at 3 months to 1.0 ± 0.4 at 24 months ( P < .011). The MOCART score increased from 51.6 ± 15 points to 72.4 ± 12.2 points ( P < .001). Improvement of the T2 index over time significantly correlated with aggrecan, COL1A1, COL2A1, and versican expression ( rs = 0.9, P < .001; rs = 0.674, P < .012; rs = 0.553, P < .05; and rs = 0.575, P < .04, respectively). No correlation was found for IL-1β. Conclusion: These data demonstrate that matrix production in transplanted chondrocytes affects maturation of MACT grafts in MRI 2 years after surgery.
Eccentric Exercise to Enhance Neuromuscular Control
Authors: Lepley Lindsey K. , Lepley Adam S. , Onate James A. , Grooms Dustin R. 
Published: June 2017

Context: Neuromuscular alterations are a major causal factor of primary and secondary injuries. Though injury prevention programs have experienced some success, rates of injuries have not declined, and after injury, individuals often return to activity with functionality below clinical recommendations. Considering alternative therapies to the conventional concentric exercise approach, such as one that can target neuromuscular injury risk and postinjury alterations, may provide for more effective injury prevention and rehabilitation protocols. Evidence Acquisition: Peer-reviewed sources available on the Web of Science and MEDLINE databases from 2000 through 2016 were gathered using searches associated with the keywords eccentric exercise, injury prevention, and neuromuscular control. Hypothesis: Eccentric exercise will reduce injury risk by targeting specific neural and morphologic alterations that precipitate neuromuscular dysfunction. Study Design: Clinical review. Level of Evidence: Level 4. Results: Neuromuscular control is influenced by alterations in muscle morphology and neural activity. Eccentric exercise beneficially modifies several underlying factors of muscle morphology (fiber typing, cross-sectional area, working range, and pennation angle), and emerging evidence indicates that eccentric exercise is also beneficial to peripheral and central neural activity (alpha motorneuron recruitment/firing, sarcolemma activity, corticospinal excitability, and brain activation). Conclusion: There is mounting evidence that eccentric exercise is not only a therapeutic intervention influencing muscle morphology but also targets unique alterations in neuromuscular control, influencing injury risk.