bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA repair deficiency disorders
Authors: Okamura Kohji , Sakaguchi Hironari , Sakamoto-Abutani Rie , Nakanishi Mahito , Nishimura Ken , Yamazaki-Inoue Mayu , Ohtaka Manami , Periasamy Vaiyapuri Subbarayan , Alshatwi Ali Abdullah , Higuchi Akon , Hanaoka Kazunori , Nakabayashi Kazuhiko , Takada Shuji , Hata Kenichiro , Toyoda Masashi , Umezawa Akihiro 
Published: May 2016

AbstractDisease-specific induced pluripotent stem cells (iPSCs) have been used as a model to analyze pathogenesis of disease. In this study, we generated iPSCs derived from a fibroblastic cell line of xeroderma pigmentosum (XP) group A (XPA-iPSCs), a rare autosomal recessive hereditary disease in which patients develop skin cancer in the areas of skin exposed to sunlight. XPA-iPSCs exhibited hypersensitivity to ultraviolet exposure and accumulation of single-nucleotide substitutions when compared with ataxia telangiectasia-derived iPSCs that were established in a previous study. However, XPA-iPSCs did not show any chromosomal instability in vitro, i.e. intact chromosomes were maintained. The results were mutually compensating for examining two major sources of mutations, nucleotide excision repair deficiency and double-strand break repair deficiency. Like XP patients, XPA-iPSCs accumulated single-nucleotide substitutions that are associated with malignant melanoma, a manifestation of XP. These results indicate that XPA-iPSCs may serve a monitoring tool (analogous to the Ames test but using mammalian cells) to measure single-nucleotide alterations, and may be a good model to clarify pathogenesis of XP. In addition, XPA-iPSCs may allow us to facilitate development of drugs that delay genetic alteration and decrease hypersensitivity to ultraviolet for therapeutic applications.
Pleistocene footprints show intensive use of lake margin habitats by Homo erectus groups
Authors: Roach Neil T. , Hatala Kevin G. , Ostrofsky Kelly R. , Villmoare Brian , Reeves Jonathan S. , Du Andrew , Braun David R. , Harris John W. K. , Behrensmeyer Anna K. , Richmond Brian G. 
Published: May 2016

AbstractReconstructing hominin paleoecology is critical for understanding our ancestors’ diets, social organizations and interactions with other animals. Most paleoecological models lack fine-scale resolution due to fossil hominin scarcity and the time-averaged accumulation of faunal assemblages. Here we present data from 481 fossil tracks from northwestern Kenya, including 97 hominin footprints attributed to Homo erectus. These tracks are found in multiple sedimentary layers spanning approximately 20 thousand years. Taphonomic experiments show that each of these trackways represents minutes to no more than a few days in the lives of the individuals moving across these paleolandscapes. The geology and associated vertebrate fauna place these tracks in a deltaic setting, near a lakeshore bordered by open grasslands. Hominin footprints are disproportionately abundant in this lake margin environment, relative to hominin skeletal fossil frequency in the same deposits. Accounting for preservation bias, this abundance of hominin footprints indicates repeated use of lakeshore habitats by Homo erectus. Clusters of very large prints moving in the same direction further suggest these hominins traversed this lakeshore in multi-male groups. Such reliance on near water environments and possibly aquatic-linked foods, may have influenced hominin foraging behavior and migratory routes across and out of Africa.
The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana
Authors: Ying Sheng-Hua , Liu Jing , Chu Xin-Ling , Xie Xue-Qin , Feng Ming-Guang 
Published: May 2016

AbstractAutophagy-related proteins play significantly different roles in eukaryotes. In the entomopathogenic fungus Beauveria bassiana, autophagy is associated with fungal growth and development. BbATG1 (a serine/threonine protein kinase) and BbATG8 (a ubiquitin-like protein) have similar roles in autophagy, but different roles in other processes. Disruption mutants of BbATG1 and BbATG8 had impaired conidial germination under starvation stress. The mutant ΔBbATG8 exhibited enhanced sensitivity to oxidative stress, while a ΔBbATG1 mutant did not. BbATG1 and BbATG8 showed different roles in spore differentiation. The blastospore yield was reduced by 70% and 92% in ΔBbATG1 and ΔBbATG8 mutants, respectively and the double mutant had a reduction of 95%. Conidial yield was reduced by approximately 90% and 50% in ΔBbATG1 and ΔBbATG8 mutants, respectively. A double mutant had a reduction similar to ΔBbATG1. Additionally, both BbATG1 and BbATG8 affected the levels of conidial protein BbCP15p required for conidiation. The virulence of each autophagy-deficient mutant was considerably weakened as indicated in topical and intrahemocoel injection assays and showed a greater reduction in topical infection. However, BbATG1 and BbATG8 had different effects on fungal virulence. Our data indicate that these autophagy-related proteins have different functions in fungal stress response, asexual development and virulence.
Nanomolar pyrophosphate detection and nucleus staining in living cells with simple terpyridine–Zn(II) complexes
Authors: Chao Duobin , Ni Shitan 
Published: May 2016

AbstractGreat efforts have been made to develop fluorescent probes for pyrophosphate (PPi) detection. Nucleus staining with fluorescence microscopy has been also widely investigated. But fluorescent probes for PPi detection with high sensitivity in water medium and nucleus staining with low–cost non–precious metal complexes in living cells are still challenging. Herein, we report simple terpyridine–Zn(II) complexes for selective nanomolar PPi detection over ATP and ADP in water based on aggregation induced emission (AIE) and intramolecular charge transfer (ICT). In addition, these terpyridine–Zn(II) complexes were successfully employed for nucleus staining in living cells. These results demonstrated simply obtained terpyridine–Zn(II) complexes are powerful tool for PPi detection and the development of PPi–related studies.
Membrane Topology and Structural Insights into the Peptide Pheromone Receptor ComD, A Quorum-Sensing Histidine Protein Kinase of Streptococcus mutans
Authors: Dong Gaofeng , Tian Xiao-Lin , Cyr Kayla , Liu Tianlei , Lin William , Tziolas Geoffrey , Li Yung-Hua 
Published: May 2016

AbstractQuorum sensing activation by signal pheromone (CSP) in Streptococcus mutans depends on the membrane-associated receptor ComD, which senses the signal and triggers the signaling cascade for bacteriocin production and other cell density-dependent activities. However, the mechanism of the signal recognition via the ComD receptor in this species is nearly unexplored. Here, we show that the membrane domain of the ComD protein forms six transmembrane segments with three extracellular loops, loopA, loopB and loopC. By structural and functional analyses of these extracellular loops, we demonstrate that both loopC and loopB are required for CSP recognition, while loopA plays little role in CSP detection. A deletion or substitution mutation of four residues NVIP in loopC abolishes CSP recognition for quorum sensing activities. We conclude that both loopC and loopB are required for forming the receptor and residues NVIP of loopC are essential for CSP recognition and quorum sensing activation in S. mutans.
AHP framework to assist lean deployment in Abu Dhabi public healthcare delivery system
Authors: Hussain Matloub , Malik Mohsin , Al Neyadi Hamda S. 
Published: May 2016

Purpose– The purpose of this paper is to introduce lean concept to the field of healthcare management, expands the conceptualization of lean management beyond the manufacturing companies to consider key waste reduction opportunities which are posited to be requisites to lean practices and implements the proposed framework in the three public hospitals in Abu Dhabi.Design/methodology/approach– This research is designed by decomposing complex and unstructured issue into a set of components organized in a multi-level hierarchical form. To deal with this complexity of multi criteria decision-making process, analytical hierarchical process (AHP) method is used in this research.Findings– AHP framework for this study resulted in a ranking of 21 healthcare wastes based on the evaluations of local situations by experienced healthcare professionals. It has been found that management in healthcare systems of Abu Dhabi is putting more emphasis on the inventory waste.Research limitations/implications– The future directions of the research would be to apply a lean set of tools for the value stream optimization of the prioritized key improvement areas.Practical implications– This is a contribution to the continuing research into lean management, giving practitioners and designers a practical way for measuring and implementing lean practices across health organizations.Originality/value– The contribution of this research, through successive stages of data collection, measurement analysis and refinement, is a set of reliable and valid framework that can be subsequently used in conceptualization, prioritization of the waste reduction strategies in healthcare management.
Reliability estimation for aluminum alloy welded joint with automatic image measurement of surface crack growth
Authors: Huang Peng Qizi , Liu Tianyu , Sun Quan , Huang Wenwei 
Published: May 2016

Purpose
– As an important connecting component, the reliability of aluminium alloy welded joints influences the whole structural effectiveness and stability of equipment. The purpose of this paper is to propose a novel reliability estimation approach to the welded joints based on time-transformed Wiener process with automatic image measurement of crack growth. The crack length information of the welded joints is incorporated into reliability analysis to reflect the product time-varying characteristics.

Design/methodology/approach
– The proposed approach is superior to other crack growth estimations in that it innovatively introduce a non-contact and flexible photogrammetry technique.First, on-line crack growth images of aluminium alloy welded joints are acquired by the designed monitor system. Second, crack length is calculated with image measurement, then the crack growth data during the manufacturing process is prepared. Finally, a time-transformed Wiener process is used to modeling the degradation, and reliability estimation is carried out with Wiener model. The approach has been validated on five 7075-T7351 welded joint samples.

Findings
– The method has a twofold task: first, the extraction of crack length growth data by a sequence of image processing. The main step is to model the crack skeleton with crack skeleton tree, and remove it edges to calculate the length of crack; second, the prediction of crack growth and reliability estimation.

Research limitations/implications
– The limitation of proposed method should not be ignored. The pixel/mm scale should be calibrated in advance that means once we have built the monitor system, the relative position of the CCD camera and the surveyed crack cannot change anymore. It has reduced the flexibility. To improve this, we can obtain binocular vision in crack image measurement. The 3-D measurements could solve calibration problem and provide more information, such as the depth and the orientation of crack to research. Therefore, future work can be centered on the improvement of monitor system and measurement precision.

Originality/value
– In the paper a novel method to estimate reliability of crack growth from welded joint based on image measurement has been presented. This method could be widely applied in different filed of manufacturing systems, reliability engineering and structural analysis.
Inverse system method for dynamic loads identification via noisy measured dynamic responses
Authors: Wang Lei , Wang Xiaojun , Li Xiao 
Published: May 2016

Purpose
– The purpose of this paper is to focus on the influences of the uncertain dynamic responses on the reconstruction of loads.

Design/methodology/approach
– Based on the assumption of unknown-but-bounded (UBB) noise, a time-domain approach to estimate the uncertain time-dependent external loads is presented by combining the inverse system method in modern control theory and interval analysis in interval mathematics. Inspired by the concept of set membership identification in control theory, an interval analysis model of external loads time history, which is indeed a region or feasible set containing all possible loads being consistent with the bounded structural acceleration responses is established and further solved by two interval algorithms.

Findings
– Unlike traditional loads identification methods which only give a point estimation, an interval estimation of external loads time history, which is a region containing all the possible loads being consistent with the uncertain structural responses, is determined. The correlation characteristics among the responses of acceleration, velocity, and displacement are also discussed in consideration of the UBB uncertainty.

Originality/value
– For one hand, the solution of the inverse problem in original system is transformed to the solution of the direct problem in inverse system; for another, the authors deal with the uncertainty by use of interval analysis method, and the identified interval process, which contains any possible external loads time history being consistent with the bounded structural responses can be approximately obtained.
Calibrating the microscopic properties of quartz sand with coupled CFD-DEM framework
Authors: Derakhshani Sayed M. , Schott Dingena L. , Lodewijks Gabriel 
Published: May 2016

Purpose– The macroscopic properties of dried sand can be correctly modelled when the accurate determination of the microscopic properties is available. The microscopic properties between the particles such as the coefficients of rolling (µr) and sliding (µs), are numerically determined in two different ways: with and without considering the fluid effect. In an earlier study, the microscopic properties were determined by discrete element method (DEM) and without considering the air effect on the macroscopic properties such as the Angle of Repose. The purpose of this paper is to recalibrate the microscopic properties through a coupling between the DEM and computational fluid dynamics (CFD).Design/methodology/approach– The first step is dedicated to the calibration of the CFD-DEM model through modelling a single particle sedimentation within air, water, and silicon oil. The voidage and drag models, the grid size ratio (D/dx), the domain size ratio (W/D), and the optimum coupling interval between the CFD and DEM were investigated through comparing the CFD-DEM results with the analytical solution and experimental data. The next step is about modelling an Hourglass with the calibrated CFD-DEM model to recalibrate theµrandµsof dried sand particles.Findings– It was concluded that the air has a minor effect on the macroscopic properties of the dried sand and theµrandµsthat were obtained with the DEM can be utilized in the CFD-DEM simulation.Originality/value– Utilizing the granulometry of dried quartz sand in the calibration process of the CFD-DEM method has raised the possibility of using theµrandµsfor other applications in future studies.
Using fuzzy logic control approach and model reduction for solving frictional contact problems
Authors: Do H , Massa F , Tison T 
Published: May 2016

Purpose– The purpose of this paper is to expand the previously published fuzzy logic controller for contact method to normal frictionless contact for solving mechanical frictional contact problems. The secondary aim is to integrate a reduction model for each component in contact to decrease the size of the global finite element contact problem.Design/methodology/approach– The proposed strategy relies on the design of two fuzzy logic controllers currently used in the automation domain. These controllers are considered to link normal and tangential gaps (for sticking conditions) with normal and tangential contact loads. A direct consequence of integrating a control-based approach into the numerical solving approach is the decomposition of the non-linear problem into a set of linear problems.Findings– With this new strategy, no tangent or coupling matrix is defined for the contact problem that allows to consider a projection matrix to reduce the size of each component in contact and subsequently to decrease the associated computational time. As in condensation techniques, this matrix is composed of both modal bases of each component in contact and static modes that capture behaviors at the contact interface. Moreover, the proposed numerical application highlights the efficiency of the proposal in terms of computation time and precision of contact data.Research limitations/implications– The developments are currently implemented in Matlab only for 2D static numerical applications. Therefore, as obtained results are very promising in terms of precision and computational time, the objective is to complete the proposed method in future research to manage frictional contact for 3D finite element models in a dynamic context.Originality/value– In conclusion, this paper highlights the interest of studying mechanical frictional contact problems by considering fuzzy logic control approaches.
Synergistic effect of phosphorus, nitrogen and silicon on flame retardancy properties of cotton fabric treated by sol-gel process
Authors: Aksit Aysun , Onar Nurhan , Kutlu Bengi , Sergin Evren , Yakin Ismail 
Published: May 2016

Purpose
– The purpose of this paper is to develop the flame retardancy properties of cotton fabrics with treatment of phosphorus and nitrogen containing silane-based nanosol by sol-gel process.

Design/methodology/approach
– Nanosols containing tetraethoxysilane or (3-aminopropyl) triethoxysilane as precursors, (3-glycidyloxypropyl) trimethoxysilane as cross-linking agent and guanidine phosphate monobasic as flame retarding agent were impregnated on cotton fabrics. Flame retardancy properties of the fabric samples were determined by limited flame spread test and limited oxygen index (LOI) test. In addition, microstructural and surface morphological properties of the fabric samples were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscope.

Findings
– Depending on the limited flame spread test, the authors show that the coated fabric samples gain flame retardancy properties and the LOI value of the samples increased as to 45.7 per cent by the synergistic effect of phosphorus-nitrogen-silicon.

Originality/value
– There have some studies in flame retardancy behaviour of textiles. In this study, flame retardant cotton fabric with very low weight in grams was improved by sol-gel process. Moreover, ecological process was provided thanks to using halogen-free flame retardant.
In-situ coated of Ag, ZnO, Ag/ZnO composite nano particles to the technical fiber by hydrothermal method
Authors: Kucuk Demet , Balci Onur , Tutak Mustafa 
Published: May 2016

Purpose
– Nowadays, the usage of antibacterial textiles is very popular for different type of textiles. The silver (Ag) and zinc oxide (ZnO) are the most popular materials in order to improve antibacterial properties of textiles. The purpose of this paper is to investigate the possibility to produce Ag nanoparticle (NP), ZnO NP, Ag/ZnO NP composite materials in this experimental study.

Design/methodology/approach
– It was investigated whether it was possible to produce Ag NP, ZnO NP, Ag/ZnO NP composite materials by hydrothermal method which was known as in-situ approach on the fiber. In addition, the colloidal silver (Ag+) was produced by electrolysis method, and used instead of process water which was necessary during generating of NPs on the fiber by this method. After whole applications, the samples were characterized by SEM, XRD, EDX analyses and the antibacterial activity of specimens was tested according to the ASTM E 2149-01 (gram-negative Escherichia coli). In addition, the resistance to the repeated washes of these antibacterial samples was investigated.

Findings
– The production of NPs on the fiber was achieved. The results showed that the samples had sufficient antibacterial activity and this activity did not reduce depending on repeated washing treatments.

Research limitations/implications
– Because of usage of one type of fiber, it would be necessary to make researches on the different type of fiber, testing procedure (with different bacteria), washing replications and prescriptions.

Practical implications
– During the process the temperature control is very important for the produced fiber. In addition chosen antibacterial test method is crucial for the testing of activity of product. Fiber must be washed at least once to remove unfixed NPs on the fiber.

Originality/value
– The technical antibacterial polyester fiber was in-situ coated by hydrothermal method with Ag, ZnO, Ag/ZnO composite NPs.
Bi-ethnic infant thermal care beliefs in Bradford, UK
Authors: Cronin de Chavez Anna , Ball Helen Louise , Ward-Platt Martin 
Published: May 2016

Purpose
– Overheating is considered a modifiable risk factor for sudden infant death syndrome (SIDS). The purpose of this paper is to explore differences in infant thermal care beliefs between mothers of South Asian and white British origin in Bradford, UK.

Design/methodology/approach
– This study employed face-to-face interviews with semi-structured and structured questions with 51 white British and 51 South-Asian mothers in the Bradford District, UK.

Findings
– White British mothers were more concerned about overheating causing SIDS whereas South-Asian mothers were more concerned about cold causing respiratory infections. However concerns around hypothermia and chills causing colds were expressed in both groups. White British mothers were significantly more likely to be concerned about their infant getting too hot than too cold and South-Asian mothers about both heat and cold (p0.001), but white British mothers on lower incomes and with poorer education expressed concern about cold more so than their better off, better educated peers indicating a possible link to fuel poverty.

Research limitations/implications
– It was not possible to observe actual night-time practices and that South Asian as a cultural category is limited because could be regarded as too broad.

Practical implications
– Whilst there guidance available to prevent infants overheating to prevent SIDS there is little or none about infants getting cold and how temperature affects other conditions.

Social implications
– Thermal care behaviours and beliefs differ between ethnic groups. SIDS and overheating is only one concern for mothers in providing thermal care for their infants. More policy and research is needed to explore the wider impact of thermal care on infant health and survival.

Originality/value
– This topic is rarely addressed despite the wide ranging implications of heat and cold to infant well-being.