bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of food literacy among the adult population in urban Uganda and Kenya: development and validation of an East African food literacy scale
Authors: Yiga Peter , Mokaya Moses , Kiyimba Tonny , Ogwok Patrick , Kyallo Florence , Koole Janna Lena , Boedt Tessy , Matthys Christophe 
Published: September 2024

Abstract

Objective:
Food literacy (FL) is a potential approach to address the nutrition transition in Africa, but a validated tool is lacking. We developed and validated a scale to assess FL among Ugandan and Kenyan adult populations.

Design:
A mixed-method approach was applied: (1) item development using literature, expert and target group insights, (2) independent country-specific validation (content, construct, criterion and concurrent) and (3) synchronisation of the two country-specific FL-scales. Construct validity was evaluated against the prime dietary quality score (PDQS) and healthy eating self-efficacy scale (HEWSE).

Setting:
Urban Uganda and Kenya.

Participants:
Two cross-sectional cross-country surveys, adults >18 years (n = 214) and university students (n = 163), were conducted.

Results:
The initial development yielded a forty-eight-item FL-scale draft. In total, twenty-six items were reframed to fit the country contexts. Six items differed content-wise across the two FL-scales and were dropped for a synchronised East African FL-scale. Weighted kappa tests revealed no deviations in individuals’ FL when either the East African FL-scale or the country-specific FL-scales are used; 0·86 (95 % CI: 0·83, 0·89), Uganda and 0·86 (95 % CI: 0·84, 0·88), Kenya. The FL-scale showed good reliability (0·71 (95 % CI: 0·60, 0·79), Uganda; 0·78 (95 % CI: 0·69, 0·84), Kenya) and positively correlated with PDQS (r = 0·29 P = 0·003, Uganda; r = 0·26 P < 0·001, Kenya) and HEWSE (r = 0·32 P < 0·001, Uganda; r = 0·23, P = 0·017, Kenya). The FL-scale distinguishes populations with higher from those with lower FL (β = 14·54 (95 % CI: 10·27, 18·81), Uganda; β = 18·79 (95 % CI: 13·92, 23·68), Kenya).

Conclusion:
Provided culture-sensitive translation and adaptation are done, the scale may be used as a basis across East Africa.
Evaluation of yield-attributing parameters in Aus rice for enhancing productivity
Authors: Mahmud Apple , Islam Md. Nahidul , Islam A. K. M. Aminul , Islam Md. Moshiul , Ghosh Uttam Kumar , Hossain Md. Saddam , Sheikh Afzal , Rahman Md. Hasan Sofiur , Tran Lam-Son Phan , Khan Md. Arifur Rahman 
Published: September 2024

AbstractThis research aimed to assess the agronomic performance of the progeny (F3 and F4 generations) of 48 newly developed Aus rice lines, using a randomized-complete-block-design under rainfed conditions. We found a wide range of variations in yield and yield-contributing traits among the studied genotypes. High board sense heritability percentages were found for sterility percentage (99.50 and 97.20), thousand-grain-weight (88.10 and 90.20 g), plant-height (84.90 and 86.90 cm) and day-to-maturity (84.50 and 97.60 d) in both F3 and F4 generations, respectively. However, the highest genetic advance as mean percentage was observed for sterility (48.00 and 50.60), effective tillers number per hill (ET) (44.70 and 47.10), total tillers number per hill (TT) (43.00 and 45.40) and filled-grains per panicle (41.00 and 43.20) respectively. Notably, the correlation study also identified the traits, TT (r = 0.31 and 0.45), ET (r = 0.30 and 0.44), straw yield (r = 0.57 and 0.39) and harvest index (r = 0.63 and 0.67) as effective for improving grain yield in both F3 and F4 generations, respectively. We identified higher grain yield per hill (g) and shorter to moderate crop growth duration (days) in several distinct accessions, including R1-49-7-1-1, R3-26-4-3-1, R1-6-2-3-1, R1-13-1-1-1, R1-50-1-1-1, R3-49-4-3-1, R1-47-7-3-1, R2-26-6-2-2, R3-30-1-2-1 and R1-44-1-2-1, among the 48 genotypes in both the F3 and F4 generations. A further location-specific agronomic study is recommended to assess the drought tolerance of these promising genotypes. This will further assess their suitability as potential breeding materials when developing rice varieties adapted to grow under fluctuating rainfalls conditions.
Identification of resistant sources for pod shattering in a cowpea (Vigna unguiculata L.) core collection using a modified screening system based on weighted level scores using random impact method
Authors: Bijarniya Deepak , Shafi Sadiah , Zaffar Aaqif , Riyaz Ishrat , Fatima Samreen , Zargar Sajad Majeed , Tripathi Kuldeep , Vara Prasad P. V. , Sofi Parvaze A. 
Published: September 2024

AbstractIn the present study we evaluated a core set of 254 cowpea genotypes for seven pod physical traits and shattering score using a modified weighted average screening system based on random impact assessment. There was substantial variability in all the pod physical traits and shattering score in the cowpea core collection indicating significant diversity of the material in respect of pod traits. Shattering score had a mean value of 5.39 with a range of 0–10. Out of 254 genotypes, 34 were resistant, 83 were moderately resistant, 82 were moderately susceptible and 55 were highly susceptible. Shattering score had significant negative correlation with pod length followed by pod weight, pod breadth, seeds per pod, pod wall weight and pod thickness. PCA concentrated 69.60% variability in the first two principal components with Eigen value of 4.49 for PC1 and 1.07 for PC2, mainly contributed by pod weight, pod length, pod breadth, pod thickness and pod wall weight. The conventional screening methods are based on level of shattering and do not take into account various types of shattering such as fissured, split, twisted or abscised. The present was aimed at identification of shattering resistant genotypes using a modified screening method based on weighted level averages. The study identified several genotypes highly resistant to pod shattering that can be used to develop shattering resistant cowpea varieties for sustainable cowpea farming and highlights the effectiveness of proposed screening method.
The Black Mouth Society and governance on the Great Plains
Authors: Lofthouse Jordan K. , Coyne Christopher J. 
Published: September 2024

Abstract
This paper analyses the institutional incentives and constraints of the Black Mouth Society – the traditional police of the pre-colonial Mandan and Hidatsa tribes – to understand how it successfully maintained social order without abusing power. The Black Mouth Society was a fraternal organization of middle-aged men that monitored and enforced rules created by the village council and chiefs. Two categories of institutions ensured reliable policing. First, on the front end, a long probationary period and system of unanimous consent facilitated the selection of reputable men who would wield policing power responsibly, reducing the chance of predation. However, individual Black Mouths occasionally abused their power. Therefore, on the back end, public communication created common knowledge, leading to social sanctions in the form of shame and restitution that punished abuses and limited further abuse. Thus, well-functioning self-governance, including reliable policing, is possible without a centralized state, as these tribes have demonstrated.
Predicting Seasonal Deformation Using InSAR and Machine Learning in the Permafrost Regions of the Yangtze River Source Region
Authors: Chen Jie , Lin Xingchen , Wu Tonghua , Hao Junming , Wu Xiaodong , Zou Defu , Zhu Xiaofan , Hu Guojie , Qiao Yongping , Wang Dong , Yang Sizhong , Zhang Lina 
Published: September 2024

AbstractQuantifying seasonal deformation is essential for accurately determining the thickness of the active layer and the distribution of water content within it, providing insights into the freeze‐thaw dynamics of permafrost environments and their sensitivity to climate change. Due to the limited hydraulic conductivity of the underlying permafrost, the freeze‐thaw processes are largely confined to the active layer, allowing for predictable seasonal deformations. This study employed Independent Component Analysis to isolate large‐scale seasonal deformation from Interferometric Synthetic Aperture Radar (InSAR) measurements taken from 2016 to 2020 in the Yangtze River Source Region (YRSR) of the Qinghai‐Tibet Plateau (QTP), covering 18,500 km2. We developed dedicated machine learning (ML) models that integrate these InSAR‐derived measurements with various environmental proxies. By applying these models to the YRSR, we generated a comprehensive, full‐coverage deformation map for permafrost terrains, achieving an R2 value of 0.91 and an Root Mean Squared Error of approximately 0.5 cm, thus confirming the model's strong predictability of seasonal deformation in permafrost regions. Deformation magnitude varied from less than 1 cm to over 10 cm. Our analysis suggests that terrain attributes, influenced by climate and soil conditions, are the primary factors driving these deformations. This research provides valuable insights into quantifying permafrost‐related seasonal deformation across expansive and rural landscapes. It also aids in assessing subsurface hydrological processes and the resilience and vulnerability of permafrost. The developed ML algorithm, with access to precise environmental data, is capable of forecasting seasonal deformations across the entire QTP and potentially throughout the Arctic.
Reconstruction of the Dynamics of a Catastrophic Crater Lake Outburst Flood, Changbaishan‐Tianchi Volcano
Authors: Qin Shengwu , Yao Jingyu , Li Guangjie , Zhang Lingshuai , Liu Xiaowei , Zhang Chaobiao , Li Li 
Published: September 2024

AbstractReconstruction of the catastrophic drainage following the Millennium Eruption (ME) of Changbaishan‐Tianchi volcano in 946 ± 20 CE is of great significance, as it contributes to improving the regional maximum flood record and develop rare flood risk analysis. However, limited knowledge exists concerning the failure mode, magnitude, and transport processes of the outburst flooding. In this work, we present a whole system model that describes the paleohydrology of catastrophic drainage using geological records along the downstream valley. The model encompasses the crater lake dynamics, an approximation of the breach erosion process and flood propagation downstream. The boulder competence method was used to constrain by reasonable flow parameters, while mitigating the uncertainty caused by the ambiguous geological paleostage indicators. Paleohydrologic analysis indicates that at least 1 km3 of water was released from the caldera, with the vertical breach erosion rates as high as 34 m/hr. Volcanic activity during the ME II may have directly contributed to triggering of the flood event. The local hydrodynamic response of the downstream riverbed captures the dynamic migration patterns of sediments across spatio‐temporal scales, offering a comprehensive interpretation of the specific scouring surfaces observed in the geological profile. The analysis of simulated inundation boundaries reveals that not all recorded inundations can be attributed to the crater lake outburst event. Reconstructions of megafloods based on downstream constraints on flood stage, velocity and discharge can help to infer and constrain the dynamics of dam failure mechanisms, and also contribute to our understanding of these complex paleohydrologic events.
Single-cell exome sequencing reveals polyclonal seeding and TRPS1 mutations in colon cancer metastasis
Authors: Cai Jianqiang , Zhang Weilong , Lu Yalan , Liu Wenjie , Zhou Haitao , Liu Mei , Bi Xinyu , Liu Jianmei , Chen Jinghua , Yin Yanjiang , Deng Yiqiao , Luo Zhiwen , Yang Yi , Chen Qichen , Chen Xiao , Xu Zheng , Zhang Yueyang , Wu Chaoling , Long Qizhao , Huang Chunyuan , Yan Changjian , Liu Yan , Guo Lei , Li Weihua , Yuan Pei , Jiao Yucheng , Song Wei , Wang Xiaobing , Huang Zhen , Ying Jianming , Zhao Hong 
Published: September 2024

Abstract
Liver metastasis remains the primary cause of mortality in patients with colon cancer. Identifying specific driver gene mutations that contribute to metastasis may offer viable therapeutic targets. To explore clonal evolution and genetic heterogeneity within the metastasis, we conducted single-cell exome sequencing on 150 single cells isolated from the primary tumor, liver metastasis, and lymphatic metastasis from a stage IV colon cancer patient. The genetic landscape of the tumor samples revealed that both lymphatic and liver metastases originated from the same region of the primary tumor. Notably, the liver metastasis was derived directly from the primary tumor, bypassing the lymph nodes. Comparative analysis of the sequencing data for individual cell pairs within different tumors demonstrated that the genetic heterogeneity of both liver and lymphatic metastases was also greater than that of the primary tumor. This finding indicates that liver and lymphatic metastases arose from clusters of circulating tumor cell (CTC) of a polyclonal origin, rather than from a single cell from the primary tumor. Single-cell transcriptome analysis suggested that higher EMT score and CNV scores were associated with more polyclonal metastasis. Additionally, a mutation in the TRPS1 (Transcriptional repressor GATA binding 1) gene, TRPS1 R544Q, was enriched in the single cells from the liver metastasis. The mutation significantly increased CRC invasion and migration both in vitro and in vivo through the TRPS1R544Q/ZEB1 axis. Further TRPS1 mutations were detected in additional colon cancer cases, correlating with advanced-stage disease and inferior prognosis. These results reveal polyclonal seeding and TRPS1 mutation as potential mechanisms driving the development of liver metastases in colon cancer.
Federated analysis of autosomal recessive coding variants in 29,745 developmental disorder patients from diverse populations
Authors: Chundru V. Kartik , Zhang Zhancheng , Walter Klaudia , Lindsay Sarah J. , Danecek Petr , Eberhardt Ruth Y. , Gardner Eugene J. , Malawsky Daniel S. , Wigdor Emilie M. , Torene Rebecca , Retterer Kyle , Wright Caroline F. , Ólafsdóttir Hildur , Guillen Sacoto Maria J. , Ayaz Akif , Akbeyaz Ismail Hakki , Türkdoğan Dilşad , Al Balushi Aaisha Ibrahim , Bertoli-Avella Aida , Bauer Peter , Szenker-Ravi Emmanuelle , Reversade Bruno , McWalter Kirsty , Sheridan Eamonn , Firth Helen V. , Hurles Matthew E. , Samocha Kaitlin E. , Ustach Vincent D. , Martin Hilary C. 
Published: September 2024

AbstractAutosomal recessive coding variants are well-known causes of rare disorders. We quantified the contribution of these variants to developmental disorders in a large, ancestrally diverse cohort comprising 29,745 trios, of whom 20.4% had genetically inferred non-European ancestries. The estimated fraction of patients attributable to exome-wide autosomal recessive coding variants ranged from ~2–19% across genetically inferred ancestry groups and was significantly correlated with average autozygosity. Established autosomal recessive developmental disorder-associated (ARDD) genes explained 84.0% of the total autosomal recessive coding burden, and 34.4% of the burden in these established genes was explained by variants not already reported as pathogenic in ClinVar. Statistical analyses identified two novel ARDD genes: KBTBD2 and ZDHHC16. This study expands our understanding of the genetic architecture of developmental disorders across diverse genetically inferred ancestry groups and suggests that improving strategies for interpreting missense variants in known ARDD genes may help diagnose more patients than discovering the remaining genes.
Mineral exploration and environmental impact assessment in the Jabal Hamadat Area, Central Eastern Desert, Egypt, using remote sensing and airborne radiometric data
Authors: Hegab Mahmoud Abd El-Rahman 
Published: September 2024

AbstractMineral exploration is essential for economic growth, but it must be conducted with careful consideration of health and environmental impacts. This study focuses on mapping potential mineralization areas and evaluating environmental consequences in the Jabal Hamadat area. By integrating ASTER, Landsat-9 and airborne radiometric data, the study includes: (1) identification of alteration mineral indicators including iron oxides (gossanized zone), chlorite (propylitic zone), kaolinite, sericite, montmorillonite/illite (argillic zone), and alunite (advanced argillic zone) via the Band Ratio (BR) technique; (2) preparation of a lineament density map through an automated lineament extraction technique; and (3) identification of areas with elevated F-parameter values exceeding 10. Ten areas with high mineralization potential are delineated for further exploration. Additionally, the study assesses environmental radiation exposure, finding that certain areas exceed the safe radiation limit of 1.0 mSv/year. Peak radiation levels range from 0.75 to 1.25 mSv/year, with mining sites showing the highest readings at 1.25 mSv/year and 0.64 mSv/year. These findings highlight elevated radiological hazards, emphasizing the need for comprehensive monitoring and effective mitigation strategies to protect human health and minimize environmental impact. The methodology's success in this area indicates its potential applicability to other mining areas, contributing to enhanced safety and environmental protection.
Ultrasonic synthesis, characterization, DFT and molecular docking of a biocompatible Zn-based MOF as a potential antimicrobial, anti-inflammatory and antitumor agent
Authors: Ismail Khaled M. , Rashidi Fatma B. , Hassan Safaa S. 
Published: September 2024

AbstractZinc metal–organic frameworks have emerged as promising candidates, demonstrating excellent biological properties stemming from the unique characteristics of MOFs and zinc. In this study, we employed a facile method to synthesize a zinc metal–organic framework [Zn(IP)(H2O)] using ultrasound irradiation, with the linker being isophthalic acid (IPA) (1,3-benzene dicarboxylic acid). The parent Zn-MOF and two Ag/Zn-MOF samples prepared via loading and encapsulation methods were comprehensively characterized using various techniques, including FT-IR, XRD, SEM, TEM, N2 adsorption–desorption isotherm, UV–vis spectroscopy and TGA. The parent Zn-MOF and two Ag/Zn-MOF samples exhibited a broad spectrum of antibacterial effects. Remarkably, genomic DNA of P. aeruginosa was effectively degraded by Zn-MOF, further supporting its potent antibacterial results. The free radical inhibition assay demonstrated a 71.0% inhibition under the influence of Zn-MOF. In vitro cytotoxicity activity of Zn-MOF against HepG-2 and Caco-2 cell lines revealed differential cytotoxic effects, with higher cytotoxicity against Caco-2 as explored from the IC50 values. This cytotoxicity was supported by the high binding affinity of Zn-MOF to CT-DNA. Importantly, the non-toxic property of Zn-MOF was confirmed through its lack of cytotoxic effects against normal lung cell (Wi-38). The anti-inflammatory treatment of Zn-MOF achieved 75.0% efficiency relative to the standard Ibuprofen drug. DFT and docking provided insights into the geometric stability of Zn-MOF and its interaction with active amino acids within selected proteins associated with the investigated diseases. Finally, the synthesized Zn-MOF shows promise for applications in cancer treatment, chemoprevention, and particularly antibacterial purposes.
Increased projected changes in quasi-resonant amplification and persistent summer weather extremes in the latest multimodel climate projections
Authors: Guimarães Sullyandro O. , Mann Michael E. , Rahmstorf Stefan , Petri Stefan , Steinman Byron A. , Brouillette Daniel J. , Christiansen Shannon , Li Xueke 
Published: September 2024

AbstractHigh-amplitude quasi-stationary atmospheric Rossby waves with zonal wave numbers 6–8 associated with the phenomenon of quasi-resonant amplification (QRA) have been linked to persistent summer extreme weather events in the Northern Hemisphere. QRA is not well-resolved in current generation climate models, therefore, necessitating an alternative approach to assessing their behavior. Using a previously-developed fingerprint-based semi-empirical approach, we project future occurrence of QRA events based on a QRA index derived from the zonally averaged surface temperature field, comparing results from CMIP 5 and 6 (Coupled Model Intercomparison Project). There is a general agreement among models, with most simulations projecting substantial increase in QRA index. Larger increases are found among CMIP6-SSP5-8.5 (42 models, 46 realizations), with 85% of models displaying a positive trend, as compared with 60% of CMIP5-RCP8.5 (33 models, 75 realizations), with a reduced spread among CMIP6-SSP5-8.5 models. CMIP6-SSP3-7.0 (23 models, 26 realizations) simulations display qualitatively similar behavior to CMIP6-SSP5-8.5, indicating a substantial increase in QRA events under business-as-usual emissions scenarios, and the results hold regardless of the increase in climate sensitivity in CMIP6. Projected aerosol reductions in CMIP6-SSP3-7.0-lowNTCF (5 models, 16 realizations) lead to halting effect in QRA index and Arctic Amplification during the 1st half of the twenty-first century. Our analysis suggests that anthropogenic warming will likely lead to an even more substantial increase in QRA events (and associated summer weather extremes) than indicated by past analyses.
Gliding motility of the diatom Craspedostauros australis coincides with the intracellular movement of raphid-specific myosins
Authors: Davutoglu Metin G. , Geyer Veikko F. , Niese Lukas , Soltwedel Johannes R. , Zoccoler Marcelo L. , Sabatino Valeria , Haase Robert , Kröger Nils , Diez Stefan , Poulsen Nicole 
Published: September 2024

AbstractRaphid diatoms are one of the few eukaryotes capable of gliding motility, which is remarkably fast and allows for quasi-instantaneous directional reversals. Besides other mechanistic models, it has been suggested that an actomyosin system provides the force for diatom gliding. However, in vivo data on the dynamics of actin and myosin in diatoms are lacking. In this study, we demonstrate that the raphe-associated actin bundles required for diatom movement do not exhibit a directional turnover of subunits and thus their dynamics do not contribute directly to force generation. By phylogenomic analysis, we identified four raphid diatom-specific myosins in Craspedostauros australis (CaMyo51A-D) and investigated their in vivo localization and dynamics through GFP-tagging. Only CaMyo51B-D but not CaMyo51A exhibited coordinated movement during gliding, consistent with a role in force generation. The characterization of raphid diatom-specific myosins lays the foundation for unraveling the molecular mechanisms that underlie the gliding motility of diatoms.