bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does competition with wind‐pollinated species alter Echium plantagineum's attractiveness to a common pollinator Bombus terrestris?
Authors: FLACHER FLORIANE , HANSART AMANDINE , MOTARD ERIC , FOFANA ABDOU MOUTALAB , VINCENT OCEANE , GESLIN BENOIT , DAJOZ ISABELLE , RAYNAUD XAVIER 
Published: June 2017

1. In insect‐pollinated plants, pollinator attraction is influenced by flowers (e.g. number, size) and their associated rewards (e.g. pollen, nectar). These traits can depend on plant interactions. Indeed, below‐ground competition between plants can lead to a decrease in flower or reward production in insect‐pollinated species.2. Wind‐pollinated plants, in particular, which are almost never studied in plant–pollinator networks, can alter insect‐pollinated plants' attractiveness through competition for nutrients. The response of pollinators to such changes has never been investigated.3. A pot experiment was carried out in which an insect‐pollinated species, Echium plantagineum, was grown in binary mixture with three wind‐pollinated species selected to exert a panel of competitive interactions. Below‐ground competition was controlled using dividers limiting interspecific root competition. Floral traits of E. plantagineum (i.e. flower production, floral display size, flower size and nectar production) were measured. For each species mixture, the visits (i.e. first visit, number of visits, 10‐min sequences) of Bombus terrestris individuals released in a flight cage containing two pots were followed, one with and one without below‐ground competition.4. Below‐ground competition significantly affected nectar's sucrose concentration but did not influence flower and nectar production. Likewise, pollinator visits were not influenced by below‐ground competition. Competitor identity significantly influenced flower and reward production of E. plantagineum, with a decrease in the presence of the most competitive wind‐pollinated species. A tendency for faster flower visitation events was also detected in the presence of the least competitive competitor. This study raises new questions regarding the influence of wind‐pollinated plants on plant–pollinator interactions.
The spectrum of aseptic central nervous system infections in southern Germany – demographic, clinical and laboratory findings
Authors: Kaminski M. , Grummel V. , Hoffmann D. , Berthele A. , Hemmer B. 
Published: June 2017

Background and purposeAseptic infections of the central nervous system (CNS) are frequently observed in Germany. However, no study has systematically addressed the spectrum of aseptic CNS infections in Germany.MethodsData on 191 adult patients diagnosed from January 2007 to December 2014 with aseptic meningitis or encephalitis/meningoencephalitis at our hospital were collected by chart review and analyzed for demographic, clinical and laboratory findings. Patients were stratified according to the causative virus and findings were compared between groups.ResultsIn our cohort, meningitis was caused in 36% by enterovirus (EV), 15% by herpes simplex virus (HSV), 12% by varicella zoster virus (VZV) and 5% by tick borne encephalitis (TBE). Encephalitis/meningoencephalitis was caused in 13% by HSV, 13% by VZV, and three out of 11 tested patients were positive for TBE. The highest incidence of EV infections was between 25 and 35 years and of HSV infections between 30 and 60 years. VZV infections had a bimodal distribution peaking below 30 and above 70 years. VZV and EV infections were more frequently observed during summer, whereas HSV infections showed no seasonal preference. Inflammatory changes in cerebrospinal fluid (CSF) were highest in HSV and lowest in EV infections.ConclusionsPolymerase chain reaction tests for HSV, VZV and EV in CSF and TBE serology determined the causative virus in over 60% of tested patients. The age of affected patients, seasonal distribution, disease course and inflammatory changes in CSF differ between groups of patients affected by the most common viral infections.
Discontinuation and non‐publication of neurodegenerative disease trials: a cross‐sectional analysis
Authors: Stefaniak J. D. , Lam T. C. H. , Sim N. E. , Al‐Shahi Salman R. , Breen D. P. 
Published: June 2017

Background and purposeTrial discontinuation and non‐publication represent major sources of research waste in clinical medicine. No previous studies have investigated non‐dissemination bias in clinical trials of neurodegenerative diseases.MethodsClinicalTrials.gov was searched for all randomized, interventional, phase II–IV trials that were registered between 1 January 2000 and 31 December 2009 and included adults with Alzheimer's disease, motor neurone disease, multiple sclerosis or Parkinson's disease. Publications from these trials were identified by extensive online searching and contact with authors, and multiple logistic regression analysis was performed to identify characteristics associated with trial discontinuation and non‐publication.ResultsIn all, 362 eligible trials were identified, of which 12% (42/362) were discontinued. 28% (91/320) of completed trials remained unpublished after 5 years. Trial discontinuation was independently associated with number of patients (P = 0.015; more likely in trials with ≤100 patients; odds ratio 2.65, 95% confidence interval 1.21–5.78) and phase of trial (P = 0.009; more likely in phase IV than phase III trials; odds ratio 3.90, 95% confidence interval 1.41–10.83). Trial non‐publication was independently associated with blinding status (P = 0.005; more likely in single‐blind than double‐blind trials; odds ratio 5.63, 95% confidence interval 1.70–18.71), number of centres (P = 0.010; more likely in single‐centre than multi‐centre trials; odds ratio 2.49, 95% confidence interval 1.25–4.99), phase of trial (P = 0.041; more likely in phase II than phase IV trials; odds ratio 2.88, 95% confidence interval 1.04–7.93) and sponsor category (P = 0.001; more likely in industry‐sponsored than university‐sponsored trials; odds ratio 5.05, 95% confidence interval 1.87–13.63).ConclusionsThere is evidence of non‐dissemination bias in randomized trials of interventions for neurodegenerative diseases. Associations with trial discontinuation and non‐publication were similar to findings in other diseases. These biases may distort the therapeutic information available to inform clinical practice.
Responses of benthic algal communities and their traits to experimental changes in fine sediments, nutrients and flow
Authors: Neif Érika M. , Graeber Daniel , Rodrigues Liliana , Rosenhøj‐Leth Simon , Jensen Tinna M. , Wiberg‐Larsen Peter , Landkildehus Frank , Riis Tenna , Baattrup‐Pedersen Annette 
Published: June 2017

Summary

Lowland stream ecosystems are subjected to multiple anthropogenic stressors, usually nutrient enrichment in combination with sedimentation of fine particles and low flow periods in summer. Here, we investigated the temporal development of the benthic algae community in response to these three stressors and linkages to the trait characteristics of the community to explore the mechanisms responsible for stress‐induced community changes.

We investigated the response of benthic algae species composition, traits (life forms, cell size categories), biovolume and chlorophyll a (Chl‐a) concentration to low flow in combination with nutrient enrichment and fine sedimentation in twelve large outdoor stream flumes (12 m long) resembling small streams in size and habitat characteristics. The experiment consisted of two phases: a normal‐flow phase followed by a low‐flow phase (90% current velocity reduction), each spanning 4 weeks. We applied a eutrophication scenario (mean increases of 1.14–5.48 mg N/L and 0.01–0.06 mg P/L in the flumes for dissolved inorganic nitrogen and phosphate respectively) throughout the experiment. Under low flow, we supplemented this with a fine sedimentation scenario (>90% stream bed cover). We took samples once in the normal‐flow phase and every week during the low‐flow phase.

We observed strong responses in the benthic algae community to sudden changes in low flow and fine sedimentation, mediating rapid species turnover with a decreased algal biovolume and increased abundance of large, motile species. However, we did not observe any pronounced responses to nutrient enrichment. In contrast to the observations for other variables, we found a continuous increase in Chl‐a concentration during low flow. This was likely due to continuous fine sedimentation during this phase, reducing light availability which probably resulted in an increase of cell‐level Chl‐a concentration in response to light limitation and lower rates of light‐induced Chl‐a degradation.

The rapid response of the benthic algal community to the applied stressors suggests that even short periods of major stressor exposure may significantly affect benthic algae in lowland systems. We suggest that short‐term stress events may have cascading effects on several important ecosystem processes given the importance of benthic algae for the productivity of these systems.
Female GADD34 mice develop age‐related inflammation and hepatocellular carcinoma
Authors: Nishio Naomi , Hasegawa Tadao , Tatsuno Ichiro , Isaka Masanori , Isobe Ken‐ichi 
Published: June 2017

AimTo analyze the impact of sex on GADD34 function, we studied the aging of female GADD34‐deficient mice and compared them with male GADD34‐deficient mice.MethodsWe used GADD34‐deficient mice on a C57BL/6 background. These mice were fed a normal diet throughout their life. Alternatively, they were fed a high‐fat diet at 3 months‐of‐age. Liver tissues taken from mice were analyzed by hematoxylin–eosin staining and immunohistochemical methods. Fresh liver cells were analyzed by flow cytometry.ResultsWe found that female GADD34‐deficient mice did not develop obesity or fatty livers. However, female GADD34‐deficient mice had infiltrations of myeloid cells in the liver, followed by liver atrophy. Many female GADD34‐deficient mice developed hepatocellular carcinoma, whereas female wild‐type (WT) mice did not show hepatocellular carcinoma during aging. Female GADD34‐deficient mice and female WT mice developed the same percentages of lymphoma. Although a high‐fat diet induced a higher level of steatosis in young male GADD34‐deficient mice compared with WT mice, a high‐fat diet induced the same level of steatosis in young female GADD34‐deficient mice compared with WT mice. However, GADD34‐deficient female young mice had a higher level of infiltration of myeloid cells and myofibroblasts than WT mice.ConclusionsIn contrast to male GADD34‐deficient mice, female GADD34‐deficient mice did not show obesity as they aged. However, similar to the males, they developed inflammation followed by hepatocellular carcinoma. Geriatr Gerontol Int 2017; 17: 2593–2601.
Retracted: National home infusion teaching guidelines will improve quality of care and patient outcomes in haemophilia treatment centres across the USA
Authors: Santaella M. E. , Bloomberg M. C. , Anglade D. 
Published: June 2017

M. E. Santaella, M. C. Bloomberg, D. AngladeThe above article, published online on 21 June 2017 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editors in Chief, Michael Makris and Craig Kessler, John Wiley & Sons Ltd and the authors. The retraction has been agreed due to the duplicated publication in the journal Research and Practice in Thrombosis and Haemostasis.
References
1. Santaella ME, Bloomberg MC, Anglade D. National home infusion teaching guidelines will improve quality of care and patient outcomes in haemophilia treatment centres across the USA. Haemophilia. 2017. https://doi.org/10.1111/hae.132892. Santaella ME, Bloomberg M, Anglade D. Home infusion teaching practices at federally funded hemophilia treatment centers in the United States of America. Res Pract Thromb Haemost. 2017;1:81–89. https://doi.org/10.1002/rth2.12020
Desmopressin in haemophilia: The need for a standardised clinical response and individualised test regimen
Authors: Stoof S. C. M. , Schütte L. M. , Leebeek F. W. G. , Cnossen M. H. , Kruip M. J. H. A. 
Published: June 2017

IntroductionDue to interindividual variation in desmopressin response, non‐severe haemophilia A patients require desmopressin testing prior to therapeutic treatment. However, adequate response or frequency of blood sampling is not standardised in international guidelines. Consequently, various definitions and blood sampling protocols are currently applied. Interestingly, sustainability of desmopressin response is not incorporated into these definitions.AimTo study desmopressin response rates in a cohort of non‐severe haemophilia A patients using currently accepted desmopressin response definitions. This, in order to formulate a standardised, uniform response which includes information on sustainability and to design a standardised blood sampling protocol.MethodsCurrently used desmopressin responses in non‐severe haemophilia A patients were derived from a literature search. Actual desmopressin response rates were individualised in 105 non‐severe HA patients from the Erasmus University Medical Centre and classified according to current varying definitions.ResultsFive response definitions were evaluated, three of which included only factor VIII (FVIII):C cut‐off levels and two also incorporated FVIII:C‐fold increase over baseline. FVIII:C‐fold increase showed no association with desmopressin response sustainability. FVIII:C 1 hour after infusion (<0.30, ≥0.30‐0.49, ≥0.50‐0.79 and ≥0.80 IU/mL) was, however, indicative of desmopressin response after 6 hours.ConclusionWe suggest standardised desmopressin response based on clinically relevant FVIII:C levels, e.g. 0.30 and 0.50 IU/mL. In addition, patients with <0.30 IU/mL FVIII:C after 1 hour (non‐responder) or ≥0.80 IU/mL (sustained responder) do not require subsequent blood sampling. However, patients with ≥0.30‐0.79 IU/mL FVIII:C after 1 hour should undergo blood sampling after 6 hours to additionally determine response sustainability.
Understanding the influences and impact of patient‐clinician communication in cancer care
Authors: Lafata Jennifer Elston , Shay Laura A. , Winship Jodi M. 
Published: June 2017

AbstractBackgroundPatient‐clinician communication is thought to be central to care outcomes, but when and how communication affects patient outcomes is not well understood.ObjectiveWe propose a conceptual model and classification framework upon which the empirical evidence base for the impact of patient‐clinician communication can be summarized and further built.DesignWe use the proposed model and framework to summarize findings from two recent systematic reviews, one evaluating the use of shared decision making (SDM) on cancer care outcomes and the other evaluating the role of physician recommendation in cancer screening use.Key resultsUsing this approach, we identified clusters of studies with positive findings, including those relying on the measurement of SDM from the patients’ perspective and affective‐cognitive outcomes, particularly in the context of surgical treatment decision making. We also identify important gaps in the literature, including the role of SDM in post‐surgical treatment and end‐of‐life care decisions, and those specifying particular physician communication strategies when recommending cancer screening.ConclusionsTransparent linkages between key conceptual domains and the influence of methodological approaches on observed patient outcomes are needed to advance our understanding of how and when patient‐clinician communication influences patient outcomes. The proposed conceptual model and classification framework can be used to facilitate the translation of empirical evidence into practice and to identify critical gaps in knowledge regarding how and when patient‐clinician communication impacts care outcomes in the context of cancer and health care more broadly.
A new system for classifying accessory canal morphology
Authors: Ahmed H. M. A. , Neelakantan P. , Dummer P. M. H. 
Published: June 2017

AbstractThorough knowledge of anatomical complexities of the root canal system has a direct impact on the effectiveness of canal preparation and filling, and is an essential prerequisite for successful root canal treatment. A wide range of complex variations in root canal anatomy exists, including root canal configuration type, developmental anomalies and minor canal morphology such as accessory canals and apical deltas. Accessory canals and apical deltas have been associated with pulp disease, primary canal infection, canal reinfection and post‐treatment disease. The current definitions of accessory canal anatomy are not standardized and potentially confusing. Given their role in endodontic disease and their impact on treatment outcomes, there is a need to have a simple classification of their anatomy to provide an accurate description of their position and path from the canal to the external surface of the root. The purpose of this article is to introduce a new system for classifying accessory canal morphology for use in research, clinical practice and training.
Targeting risk factors for type 2 diabetes in American Indian youth: the Tribal Turning Point pilot study
Authors: Sauder K. A. , Dabelea D. , Bailey‐Callahan R. , Kanott Lambert S. , Powell J. , James R. , Percy C. , Jenks B. F. , Testaverde L. , Thomas J. M. , Barber R. , Smiley J. , Hockett C. W. , Zhong V. W. , Letourneau L. , Moore K. , Delamater A. M. , Mayer‐Davis E. 
Published: June 2017

SummaryBackgroundAmerican Indian (AI) youth are at high risk for type 2 diabetes.ObjectivesTo partner with Eastern Band of Cherokee Indians and Navajo Nation to develop a culturally sensitive behavioural intervention for youth (Tribal Turning Point; TTP) and assess feasibility in an 8‐month randomized pilot study.MethodsWe enrolled 62 overweight/obese AI children (7–10 years) who participated with ≥1 parent/primary caregiver. Intervention participants (n = 29) attended 12 group classes and five individual sessions. Control participants (n = 33) attended three health and safety group sessions. We analysed group differences for changes in anthropometrics (BMI, BMI z‐score, waist circumference), cardiometabolic (insulin, glucose, blood pressure) and behavioural (physical activity and dietary self‐efficacy) outcomes.ResultsStudy retention was 97%, and intervention group attendance averaged 84%. We observed significant treatment effects (p = 0.02) for BMI and BMI z‐score: BMI increased in control (+1.0 kg m−2, p < 0.001) but not intervention participants (+0.3 kg m−2, p = 0.13); BMI z‐score decreased in intervention (−0.17, p = 0.004) but not control participants (0.01, p = 0.82). There were no treatment effects for cardiometabolic or behavioural outcomes.ConclusionsWe demonstrated that a behavioural intervention is feasible to deliver and improved obesity measures in AI youth. Future work should evaluate TTP for effectiveness, sustainability and long‐term impact in expanded tribal settings.
Molecular cloning, mRNA expression and biological activity of the pheromone biosynthesis activating neuropeptide (PBAN) from the European corn borer, Ostrinia nubilalis
Authors: Fodor J. , Köblös G. , Kákai Á. , Kárpáti Z. , Molnár B. P. , Dankó T. , Bozsik G. , Bognár C. , Szőcs G. , Fónagy A. 
Published: June 2017

AbstractPheromone biosynthesis activating neuropeptide (PBAN) is a member of the pyrokinin (FXPRLamide) insect neuropeptides. Here, we report the cloning of the gene Ostnu‐PBAN from the E and Z pheromone strains of the European corn borer (ECB), Ostrinia nubilalis (Lepidoptera: Crambidae), a major pest of maize. The Ostnu‐PBAN genomic sequence is > 5 kb in length and consists of six exons. The deduced amino acid sequence revealed a 200‐residue precursor protein including a signal peptide, a 24‐amino acid (aa) diapause hormone, a 37‐aa PBAN and three other FXPRLamide neuropeptides. Our in vivo assays suggest that the 37‐aa synthetic Ostnu‐PBAN is hormonally active in the pheromone gland. It restores sex pheromone production to normal levels in mated females and decapitated virgins of both E and Z cultures. The results of a real‐time PCR analysis indicated that Ostnu‐PBAN mRNA levels reached a plateau in the brain‐suboesophageal ganglion complexes 1 day after eclosion, and mating did not affect the mRNA expression. Three size classes of Ostnu‐PBAN mRNA (1.9, 2.0 and 2.1 kb) were obtained, differing only in the length of the 3′ untranslated region. However, there was no correlation between sequence divergence and the pheromone composition, voltinism or geographical origin (Hungary, Slovenia, Sweden, Turkey) of ECB moths.
Characterisation of clonal Philadelphia‐negative cytogenetic abnormalities in a large cohort of chronic myeloid leukaemia
Authors: Chen Xiangjun , Zheng Jine , Liang Kaiwei , He Yanli , Du Wen , Li Juan , Liu Wei , Hu Yanjie , Huang Shiang , Yao Junxia 
Published: June 2017

AbstractBackgroundClonal Philadelphia (Ph)‐negative cytogenetic abnormalities (CPCA) have been reported in chronic myeloid leukaemia (CML) patients treated with either interferon or tyrosine kinase inhibitor (TKI). However, the incidences and types of these cytogenetic abnormalities after treatment vary due to the limited populations enroled.MethodsWe analysed the frequency and types of CPCA in a cohort of 607 CML patients in the chronic phase after TKI treatment. We also followed up these CPCA with a median of 31.8 months (range from 11 to 63 months) from diagnosis and investigated their effects on disease progression.ResultsWe found 18 out of 607 CML patients had cytogenetic abnormality in the Ph‐negative cells with an incidence of 3%. In total, six types of chromosomal abnormalities have been identified in these 18 patients with the majority of them aneuploidy abnormalities, especially the trisomy 8. Four of 18 patients (22.2%) were noted to have several abnormalities in the Ph‐negative cells. Furthermore, follow‐up studies of these CPCA showed that they could be either persistent or transient (15 vs 3 patients), and may not affect disease progression since none of them developed transformed myelodysplasia or transformed acute myeloid leukaemia.ConclusionThree percent of CML patients in the chronic phase were observed to have CPCA during TKI treatment. Our results suggest that the detection of CPCA in CML may not predict disease progression.
A low sintering temperature glass based on SiO2–P2O5–ZnO–B2O3–R2O system for white LEDs with high color rendering index
Authors: Kim Seonghyeon , Park Hyun‐A , Im Won Bin , Heo Jong , Choi Jae‐Young , Chung Woon Jin 
Published: June 2017

AbstractA low sintering temperature glass based on the SiO2–P2O5–ZnO–B2O3–R2O (R=K and Na) system was studied as a matrix for embedding phosphors to fabricate color tunable white LEDs. The proposed system, which uses no heavy‐metal elements and can be sintered at 500°C, incorporates thermally weak commercial phosphors such as CaAlSiN3:Eu2+ to produce phosphor‐in‐glasses (PiGs). Changing the mixing ratio of glass to phosphors affected the photo‐luminescence spectra and color coordinates of the PiGs when mounted on a blue LED. The color rendering index (CRI) and color correlated temperature (CCT) of the LEDs were also varied with the mixing ratio, providing color tunable white LEDs. A high CRI, up to 93, as well as highly improved thermal stability were obtained, along with a low sintering temperature compared to other glass systems, suggesting the practical feasibility of the proposed system.