bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of miR‐203 in estrogen receptor‐mediated signaling in the rat uterus and endometrial carcinoma
Authors: Zierau Oliver , Helle Janina , Schadyew Sabina , Morgenroth Yanni , Bentler Martin , Hennig Alexander , Chittur Sridar , Tenniswood Martin , Kretzschmar Georg 
Published: January 2018

AbstractThe role of microRNAs (miRNA) in estrogen receptor (ER) signaling in the uterus and in endometrial cancer is not well understood. We therefore analyzed miRNA expression in uterine samples from a standard 3‐day uterotrophic assay using young female adult rats to identify E2‐regulated miRNAs. Microarray analysis identified 47 E2 down‐regulated miRNAs including miR‐30a, and 25 E2up‐regulated miRNAs including miR‐672, miR‐203, and miR‐146b. The strongly E2‐upregulated miR‐203 was selected for further analysis. miR‐203 was deleted in the rat endometrial adenocarcinoma cell line, RUCA‐I, using CRISPR/CAS9. Five clones devoid of miR‐203 expression were generated. Proliferation was reduced and G2‐arrest was observed in all miR‐203 deficient RUCA‐I clones. Transfection with a miR‐203‐3p mimic partially rescues this effect. Comparison of mRNA expression in three miR‐203 knockout clones to wild type RUCA‐I cells reveals 566 miR‐203‐upregulated and 592 miR‐203‐downregulated genes. 43 of the genes that are upregulated by miR‐203 knockout in vitro are downregulated in the uterus by E2. Of these Acer2, Zbtb20, Ptn, Rcbtb2, Mum1l1, Hmgn3, and Nfat5 possess one or more seed sequence matches in their 3′‐UTR that are predicted to be targets of miR‐203. These data demonstrate the importance of E2 regulated miRNAs in general, and miR‐203 in particular, for E2 regulated gene expression and physiological processes including proliferation and cell migration, in the uterus as well as in the etiology of endometrial carcinomas.
RETRACTED: MiR‐137 functions as a tumor suppressor in pancreatic cancer by targeting MRGBP
Authors: Ding Feng , Zhang Shuang , Gao Shaoyang , Shang Jian , Li Yanxia , Cui Ning , Zhao Qiu 
Published: January 2018

AbstractmiRNAs are small noncoding RNAs that act as critical epigenetic regulators in tumor carcinogenesis. In this study, our data showed that miR‐137 was significantly downregulated in 58 pairs of human pancreatic cancer (PanCa) tissues and PanCa cell lines. Furthermore, the deregulated miR‐137 was correlated with increased tumor size, higher TNM stage, and worse prognosis in pancreatic cancer. Functional studies demonstrated that overexpression of miR‐137 dramatically suppressed cell proliferation and induced cell apoptosis in vitro. Meanwhile, upregulated miR‐137 remarkably inhibited migration and invasion of pancreatic cancer cells. Further studies indicated that MRGBP was identified as the direct downstream target gene of miR‐137. In addition, MRGBP expression is significantly downregulated in miR‐137‐transfected cells. Our previous study revealed that silencing of MRGBP suppressed the growth of PanCa cells in vitro and in vivo and also promoted apoptosis, and inhibited migration and invasion of PanCa cells, which are consistent with the effects of miR‐137 overexpression. Taken together, our findings suggest that miR‐137 may function as a novel tumor promoter through directly targeting MRGBP in PanCa.
Cover Image, Volume 93, Issue 2
Authors: Li Jianfeng , Liu Xinyuan , Wang Shujun , Du Zhiping , Guo Yimin , Cheng Fangqin 
Published: January 2018

The cover image, by Jianfeng Li et al., is based on the Research Article Synthesis and optimization of a novel poly‐silicic metal coagulant from coal gangue for tertiary‐treatment of coking wastewater, DOI: 10.1002/jctb.5362.
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Responses of flocculent and granular sludge in anaerobic sequencing batch reactors (ASBRs) to azithromycin wastewater and its impact on microbial communities
Authors: Liu Peng‐yu , Chen Jia‐rong , Shao Lei , Tan Jun , Chen Dai‐jie 
Published: January 2018

AbstractBACKGROUNDAzithromycin (AZM), a 15‐member macrolide antibiotic, has caused widespread pollution in China, where anaerobic digestion is the predominant wastewater treatment technology. However, the type of anaerobic sludge most suitable for treating antibiotic wastewater is unclear. Therefore, this study aimed to determine the response of anaerobic flocculent and granular sludge to AZM wastewater, and, using a high‐throughput sequencing technique, to explain the diverse microbial community structures.RESULTSAnaerobic granular sludge provided better resistance to the decline of chemical oxygen demand (COD) removal caused by AZM and greater biodegradation efficiency than flocculent sludge. Furthermore, there was stronger resistance to AZM‐induced reactive oxygen species in the granular sludge than in the flocculent sludge. The granular sludge exhibited richer microbial communities than the flocculent sludge after AZM exposure to the phylum Proteobacteria and Bacteroidetes.CONCLUSIONAt high AZM concentration, anaerobic granular sludge was superior to flocculent sludge in COD removal and AZM degradation. This is attributed to less cell death and greater microbial diversity and richness in the granular sludge than in the flocculent sludge following exposure to AZM. Proteobacteria and Bacteroidetes contributed to the resistance to AZM in the granular sludge. © 2018 Society of Chemical Industry
Transplantation or rurality? Migration and HIV risk among Chinese men who have sex with men in the urban areas
Authors: Liu Chuncheng , Fu Rong , Tang Weiming , Cao Bolin , Pan Stephen W , Wei Chongyi , Tucker Joseph D , Kumi Smith M. 
Published: January 2018

AbstractIntroductionMigration of men who have sex with men (MSM) from rural to urban areas is common across low‐ and middle‐income countries and is widely believed to contribute to elevated HIV risk among migrant MSM in urban areas. Little consensus exists on whether their risk is due to their transplantation or their being from resource‐constrained rural areas. This study seeks to clarify the relationship between migration and HIV risks by comparing differences in HIV‐related risky sexual behaviours and healthcare utilization across competing conceptualizations of migratory statuses.MethodsIn July 2016, MSM ≥16 years old currently residing in one of eight urban cities in China were recruited for an online cross‐sectional survey, which collected information on socio‐demographics, sexual behaviours, HIV care‐seeking behaviours, and healthcare utilization. Based on a question about residency status, each participant was classified as an urban local resident, urban transplant, or rural transplant. Multivariable multinomial logistic regression was used to examine the associations between risky behaviours and healthcare utilization among these three groups.ResultsAmong 2007 MSM, the proportion of local, urban transplant and rural transplant were 32% (648/2007), 24% (478/2007), and 44% (881/2007), respectively. Compared with urban local resident MSM, urban transplant MSM were more likely to have ever tested for HIV (adjusted odds ratio (aOR) = 1.39, 95% confidence interval (CI): 1.08 to 1.80). Compared with urban transplant MSM, rural transplant MSM were less likely to have utilized any governmental sexual health services in the past three months (aOR = 0.75, 95% CI: 0.60 to 0.93), ever tested for HIV (aOR = 0.77, 95% CI: 0.61 to 0.96), ever initiated antiretroviral therapy (ART) (aOR = 0.16, 95% CI: 0.05 to 0.52), and ever purchased sex (aOR = 0.57, 95% CI: 0.38 to 0.85). No other significant differences were found in sexual behaviours among three groups.ConclusionsThe widely used local/migrant categorization obscures important differences in HIV risk present between urban/rural subgroups among them. Previous studies of HIV risks in Chinese “migrant” may have failed to consider the role of structural factors such as discrimination or barriers to healthcare when interpreting their findings of higher HIV prevalence in this population. Low ART uptake among rural transplant MSM in this study is particularly concerning and underscore the need for HIV‐related interventions tailored for this group.
REnal Flow and Microstructure AnisotroPy (REFMAP) MRI in Normal and Peritumoral Renal Tissue
Authors: Liu Andrea L. , Mikheev Artem , Rusinek Henry , Huang William C. , Wysock James S. , Babb James S. , Feiweier Thorsten , Stoffel David , Chandarana Hersh , Sigmund Eric E. 
Published: January 2018

BackgroundDiffusion‐weighted imaging (DWI) provides insight into the pathophysiology underlying renal dysfunction. Variants of DWI include intravoxel incoherent motion (IVIM), which differentiates between microstructural diffusion and vascular or tubular flow, and diffusion tensor imaging (DTI), which quantifies diffusion directionality.PurposeTo investigate the reproducibility of joint IVIM‐DTI and compare controls to presurgical renal mass patients.Study TypeProspective cross‐sectional.SubjectsThirteen healthy controls and ten presurgical renal mass patients were scanned. Ten controls were scanned twice to investigate reproducibility.Field Strength/SequenceSubjects were scanned on a 3T system using 10 b‐values and 20 diffusion directions for IVIM‐DTI in a study approved by the local Institutional Review Board.AssessmentRetrospective coregistration and measurement of joint IVIM‐DTI parameters were performed.Statistical AnalysisParameter reproducibility was defined as intraclass correlation coefficient (ICC) >0.7 and coefficient of variation (CV) <30%. Patient data were stratified by lesion side (contralateral/ipsilateral) for comparison with controls. Corticomedullary differentiation was evaluated.ResultsIn controls, the reproducible subset of REnal Flow and Microstructure AnisotroPy (REFMAP) parameters had average ICC = 0.82 and CV = 7.5%. In renal mass patients, medullary fractional anisotropy (FA) was significantly lower than in controls (0.227 ± 0.072 vs. 0.291 ± 0.044, P = 0.016 for the kidney contralateral to the mass and 0.228 ± 0.070 vs. 0.291 ± 0.044, P = 0.018 for the kidney ipsilateral). In the kidney ipsilateral to the mass, cortical Dp,radial was significantly higher than in controls (P = 0.012). Conversely, medullary Dp,axial was significantly lower in contralateral than ipsilateral kidneys (P = 0.027) and normal controls (P = 0.044).Data ConclusionREFMAP‐MRI parameters provide unique information regarding renal dysfunction. In presurgical renal mass patients, directional flow changes were noted that were not identified with IVIM analysis alone. Both contralateral and ipsilateral kidneys in patients show reductions in structural diffusivities and anisotropy, while flow metrics showed opposing changes in contralateral vs. ipsilateral kidneys.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2018.
Simultaneous pharmacokinetics and stability studies of physalins in rat plasma and intestinal bacteria culture media using liquid chromatography with mass spectrometry
Authors: Zheng Yunliang , Lin Meihua , Hu Xingjiang , Zhai You , Zhang Qiao , Lou Yan , ShenTu Jianzhong , Wu Lihua 
Published: January 2018

AbstractPhysalins are the major steroidal constituent of Physalis plants and display a range of biological activities. For this study, a rapid and sensitive high‐performance liquid chromatography with triple quadrupole mass spectrometry method was developed for the simultaneous quantification of six physalins. Specifically, it was for the quantification of physalin A, physalin B, physalin D, physalin G, 4,7‐didehydroneophysalin B, and isophysalin B in rat plasma and rat intestinal bacteria. After a solid‐phase extraction, analytes and internal standards (prednisolone) were separated on a Shield reverse‐phase C18 column (measuring 3 mm × 150 mm with an internal diameter of 3.5 μm) and determined using multiple reactions in a monitoring mode with a positive‐ion electrospray ionization source. The mobile phase was a mixture of 0.1% formic acid in water (A) and acetonitrile (B) and was used at a flow rate of 0.6 mL/min. The intra‐ and interday precisions were within 15% with accuracies ranging from 86.2 to 114%. The method was validated and successfully applied to pharmacokinetics and stability studies of six physalins in rat plasma and rat intestinal bacteria, respectively. The results showed that physalin B and isophysalin B could not be absorbed by rats, and rat intestinal bacteria could quickly transform physalins.
Long‐term outcomes of cochlear implantation in patients with high‐frequency hearing loss
Authors: Roland J. Thomas , Gantz Bruce J. , Waltzman Susan B. , Parkinson Aaron J. 
Published: January 2018

ObjectiveTo demonstrate the long‐term benefits of implantation in patients with high‐frequency sensorineural hearing loss, this report provides 5‐year follow‐up on a group of implant recipients who were subjects of the Cochlear™ Nucleus® Hybrid™ L24 Implant System pivotal clinical study.MethodsThe results of three related clinical studies were compiled to provide outcome data after 1, 3, and 5 years of implant use in a group of subjects who presented with preoperative high‐frequency hearing loss and were implanted with a Nucleus Hybrid L24 (Cochlear Ltd., Sydney, Australia) cochlear implant. A subset of the 50 adult subjects (N = 32) who participated in the Hybrid L24 pivotal Investigational Device Exemption (IDE) completed comprehensive evaluations at 12 months postactivation, 3 years postactivation, and then as part of a postapproval study at 5 years postactivation. Testing included audiometric, speech perception, and subjective satisfaction measures.ResultsMean unilateral speech perception performance was significantly improved at all postoperative intervals compared to preoperative best‐aided results and has remained stable to 5 years postactivation. Ninety‐four percent of subjects had measurable hearing, and 72% continued to use electric‐acoustic stimulation in the implanted ear after 5 years of implant use. Subjective satisfaction results support objective performance improvements.ConclusionResults demonstrate long‐term success of patients with high‐frequency hearing loss following Hybrid L24 (Cochlear) cochlear implantation. Benefits include speech perception abilities significantly better than those in the preoperative best‐aided condition, with additional benefit in those using electric‐acoustic stimulation in the implanted ear.Level of Evidence2b. Laryngoscope, 1939–1945, 2018
Cleaner Cuts for Semiconductor Packaging
Authors: Thibault Florent , van Nunen Joris 
Published: January 2018

AbstractThe semiconductor packaging industry faces numerous manufacturing challenges in the production of next generation designs for advanced, miniaturized, microelectronic devices. The singulation of multiple circuits produced on a single substrate into individual devices is a standout example of this. In many instances, the cutting requirements for singulation already surpass the capabilities of mechanical cutting. Specific examples are system‐in‐package (SiP) circuitry used in wrist‐worn devices and the fingerprint sensors found in many phones, tablets or even smart watches. This leaves laser cutting as the only viable method, and both nanosecond and picosecond lasers are currently employed for these processes. This article compares these two approaches, and provides some detail on the characteristics and implementation of USP picosecond lasers for both SiP and fingerprint sensor singulation.
Why Beam Analysis is Crucial for Additive Manufacturing
Authors: Dini Christian 
Published: January 2018

AbstractWorkpieces that are lightweight, easy building of complicated structures, fast production cycles – the advantages of additive manufacturing are many and appealing. But what started out as an approach for rapid prototyping will soon be used even to produce critical parts for airplanes or space shuttles. As the number of applications grows, so do the technological requirements. This is why it is so important for the parameters of the laser beam always to be reproducible – and not only on one machine over time, but also from one machine to the next.
Noninvasive optical spectroscopy for identification of non‐melanoma skin cancer: Pilot study
Authors: Carpenter David J. , Sajisevi Mirabelle B. , Chapurin Nikita , Brown Clifford Scott , Cheng Tracy , Palmer Gregory M. , Stevenson Daniel S. , Rao Caroline L. , Hall Russell P. , Woodard Charles R. 
Published: January 2018

ObjectiveOptical spectroscopy offers a noninvasive alternative to biopsy as a first‐line screening tool for suspicious skin lesions. This study sought to define several optical parameters across malignant and benign tissue types.Study DesignProspective pilot trial utilizing the Zenalux IM1 optical spectroscopy device from April 2016 to February 2017. For each skin lesion, provider pre‐biopsy probability of malignancy was compared to histolopathologic diagnosis. Optical data were characterized across basal cell carcinoma (BCC; n = 9), squamous cell carcinoma (SCC; n = 5), actinic keratosis (AK; n = 4), scar tissue (n = 6), nevus (n = 2), and neurofibroma (NF; n = 1). Across all patients, agreement was determined between control measurements collected adjacent to the lesion and from the upper extremity.MethodsProspective single center pilot study. The optical properties of 27 cutaneous lesions were collected from 18 adult patients presenting to Otolaryngology and Dermatology clinics with suspicious skin lesions warranting biopsy. Spectroscopy measurements were recorded for each lesion: two at the lesion site, two at an adjacent site (internal control), and one at the central medial upper extremity (arm control). Variables of interest included absolute oxygenated hemoglobin (Hb), Hb saturation, total Hb concentration, and Eumelanin concentration. For each lesion, internal control averages were subtracted from lesion averages to provide delta parameter values, and lesion averages were divided by internal control averages to provide ratio parameter values.ResultsMean percent difference between pre‐biopsy probability of malignancy and histology was 29%, with a difference of 75% or greater seen in 5 of 25 lesions. Mean values for BCC, SCC, AK, and scar tissue varied most between extracted mean reduced scatter estimate (; cm−) delta values (BCC: −2.2 ± 3.8; SCC: −3.9 ± 2.0; AK: −3.3 ± 4.2, Scar: −1.7 ± 1.2) and total Hb (µM) ratio (BCC: 2.0 ± 3.3; SCC: 3.0 ± 1.3; AK: 1.1 ± 0.6; Scar: 1.4 ± 1.1). Agreement between local and arm controls was poor.ConclusionThis pilot trial utilizes optical spectroscopy as a noninvasive method for determining cutaneous lesion histology. Effect sizes observed across optical parameters for benign and malignant tissue types will guide larger prospective studies that may ultimately lead to prediction of lesional histology without need for invasive biopsy. Lasers Surg. Med. 50:246–252, 2018. © 2018 Wiley Periodicals, Inc.
New insights into emotion valence and loyalty intentions in relational exchanges
Authors: McKechnie Sally , Nath Prithwiraj , Xun Jiyao 
Published: January 2018

AbstractThis research examines how emotion valence and future intentions arising from relational exchanges with a service firm depend on a consumer's level of goal attainment and locus of causality (firm vs. self) of relational outcomes. Drawing on the theories of goal‐directed behavior and agency of causation, this study hypothesizes that levels of goal attainment and locus of causality influence the generation of positive emotions (gratitude), negative emotions (grudge and guilt), relational mediators (trust and commitment), and subsequent future intentions to remain loyal to the firm. Based on a controlled experiment with 284 subjects in a consumer‐determined relationship setting, the research finds that emotion valence and future loyalty intentions are contingent upon the fulfillment of relational objectives of individual consumers and the agency of causation for the outcome of the relational exchanges. In doing so, this study delineates the conditioning mechanism that directs how emotion valence influences behavioral intentions. The study contributes to the consumer behavior and services marketing literatures on consumption‐based emotions and has significant practice implications for relational behaviors.
Novel discretization methods for improved simulation precision in injection molding
Authors: Karyofylli V. , Behr M. , Schmitz M. , Hopmann C. 
Published: January 2018

AbstractMold filling is an important stage of injection molding, which is one of the most commonly used manufacturing processes for the production of thermoplastic components in high volumes. As a consequence, the numerical simulation of this process based on computational fluid dynamics (CFD) is of great significance for production engineering [1, 2]. However, modeling of the mold filling is a tremendously demanding process, when considering interfacial physical phenomena, such as two‐phase flows, building a sharp interface between the molten plastics and the present air in the cavity or the dynamic wetting contact line at the cavity surface. A method for dealing with these phenomena is a local mesh refinement both in space and time. In this paper, the numerical solution of a mold filling problem using simplex‐type space‐time finite elements is presented and compared with experiments. These elements can be suited to increase efficiency, when used for the aforementioned refinement. In addition, a Navier's slip boundary condition is applied to the solid boundaries of the mold allowing the contact line to evolve along the boundary, while enabling a better prediction of the pressure distribution. The presented work was performed in collaboration of the subprojects B3 and B5 of the collaborative research center 1120 “Precision Melt Engineering”.
Hybrid simulation of laser deep penetration welding
Authors: Schöler C. , Haeusler A. , Karyofylli V. , Behr M. , Schulz W. , Gillner A. , Niessen M. 
Published: January 2018

AbstractIn laser deep penetration welding, the knowledge on the temperature history of the material is of great interest for the assessment of the quality properties of the weld. For this purpose a hybrid process model that enables the fast calculation of temperature distributions as a function of process parameters is applied. The interaction between laser and material is taken into account by a reduced keyhole model, which exploits a hierarchy in the spatial dimensions occurring at high feed rates. The resulting shape of a stationary keyhole is introduced as a Dirichlet boundary into a thermal finite element simulation in which it is moved through the workpiece according to the process control of the laser beam. The boundary is mathematically described by a level set function and immersed in a fixed computational mesh. The Dirichlet boundary condition is imposed using an embedded boundary method. The calculated temperature distributions are evaluated by means of bead on plate welds conducted in 0.9 mm thick sheets of 1.4301 (AISI 304) stainless steel.
Wideband circularly polarized sectored conical dielectric resonator antenna excited by S‐shaped slot
Authors: Dash Upali Aparajita , Sahu Sudhakar 
Published: January 2018

AbstractA conical sectored dielectric resonator antenna (DRA) loaded on S‐shaped slot square patch for wideband circular polarization is investigated. Wideband circular polarization (CP) is achieved by single tab extended at right top corner of the square patch along with the introduction of sectors on the top of DRA and S‐shaped slot on the patch. Calculated size of slot along with optimized height and size of DRA through parametric study helps to get wide impedance bandwidth. In addition, optimized size of single tab gives the wide axial ratio bandwidth. The −10 dB impedance bandwidth is 100.18% (2.1–6.0 GHz) and 3‐dB axial ratio (AR) bandwidth is 69.52% (3.0–6.2 GHz). The gain variation is around 3 dBi and radiation efficiency is around 80%–95% within the AR bandwidth. The proposed model is fabricated and measured which agree well with the simulated results.