bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenic lineage of Perkinsea associated with mass mortality of frogs across the United States
Authors: Isidoro-Ayza Marcos , Lorch Jeffrey M. , Grear Daniel A. , Winzeler Megan , Calhoun Daniel L. , Barichivich William J. 
Published: August 2017

AbstractEmerging infectious diseases such as chytridiomycosis and ranavirus infections are important contributors to the worldwide decline of amphibian populations. We reviewed data on 247 anuran mortality events in 43 States of the United States from 1999–2015. Our findings suggest that a severe infectious disease of tadpoles caused by a protist belonging to the phylum Perkinsea might represent the third most common infectious disease of anurans after ranavirus infections and chytridiomycosis. Severe Perkinsea infections (SPI) were systemic and led to multiorganic failure and death. The SPI mortality events affected numerous anuran species and occurred over a broad geographic area, from boreal to subtropical habitats. Livers from all PCR-tested SPI-tadpoles (n = 19) were positive for the Novel Alveolate Group 01 (NAG01) of Perkinsea, while only 2.5% histologically normal tadpole livers tested positive (2/81), suggesting that subclinical infections are uncommon. Phylogenetic analysis demonstrated that SPI is associated with a phylogenetically distinct clade of NAG01 Perkinsea. These data suggest that this virulent Perkinsea clade is an important pathogen of frogs in the United States. Given its association with mortality events and tendency to be overlooked, the potential role of this emerging pathogen in amphibian declines on a broad geographic scale warrants further investigation.
A promising azeotrope-like mosquito repellent blend
Authors: Izadi Homa , Focke Walter W , Asaadi Erfan , Maharaj Rajendra , Pretorius Jannie , Loots Mattheüs Theodor 
Published: August 2017

AbstractTopical repellents play a key role in reducing the outdoor transmission of mosquito-borne diseases by reducing human-vector contact. Excellent repellents are available, but there is always room for improvement. This article reports on a particularly effective binary repellent blend of ethyl butylacetylaminopropionate and nonanoic acid. A composition containing 25 mol% of the acid exhibits negative pseudo-azeotrope behaviour at 50 °C, meaning that the liquid vapour pressure is lower than that of the parent compounds and evaporation occurs without a change in the liquid composition. In tests performed using the South African Medical Research Council’s cup-on-arm procedure, this mixture provided better protection for a longer time than the “gold standard of mosquito repellents”, namely N,N-diethyl-m-toluamide, commonly known as DEET.
Development and validation of a multivariate predictive model for rheumatoid arthritis mortality using a machine learning approach
Authors: Lezcano-Valverde José M. , Salazar Fernando , León Leticia , Toledano Esther , Jover Juan A. , Fernandez-Gutierrez Benjamín , Soudah Eduardo , González-Álvaro Isidoro , Abasolo Lydia , Rodriguez-Rodriguez Luis 
Published: August 2017

AbstractWe developed and independently validated a rheumatoid arthritis (RA) mortality prediction model using the machine learning method Random Survival Forests (RSF). Two independent cohorts from Madrid (Spain) were used: the Hospital Clínico San Carlos RA Cohort (HCSC-RAC; training; 1,461 patients), and the Hospital Universitario de La Princesa Early Arthritis Register Longitudinal study (PEARL; validation; 280 patients). Demographic and clinical-related variables collected during the first two years after disease diagnosis were used. 148 and 21 patients from HCSC-RAC and PEARL died during a median follow-up time of 4.3 and 5.0 years, respectively. Age at diagnosis, median erythrocyte sedimentation rate, and number of hospital admissions showed the higher predictive capacity. Prediction errors in the training and validation cohorts were 0.187 and 0.233, respectively. A survival tree identified five mortality risk groups using the predicted ensemble mortality. After 1 and 7 years of follow-up, time-dependent specificity and sensitivity in the validation cohort were 0.79–0.80 and 0.43–0.48, respectively, using the cut-off value dividing the two lower risk categories. Calibration curves showed overestimation of the mortality risk in the validation cohort. In conclusion, we were able to develop a clinical prediction model for RA mortality using RSF, providing evidence for further work on external validation.
Telemonitoring in fasting individuals with Type 2 Diabetes Mellitus during Ramadan: A prospective, randomised controlled study
Authors: Lee Jun Yang , Wong Chee Piau , Tan Christina San San , Nasir Nazrila Hairizan , Lee Shaun Wen Huey 
Published: August 2017

AbstractWe determined the impact of a remote blood glucose telemonitoring program with feedback in type 2 diabetes mellitus patients fasting during Ramadan compared to conventional self-monitoring method. A twelve-week cluster randomised study, with 85 participants who wish to fast for at least 15 days during Ramadan was conducted. Self-measurement and transmission of blood glucose results were performed six times daily during Ramadan. Results were transmitted to a secure website for review with feedback from case manager if necessary. The control group received usual care. The main outcome was the number of participants experiencing hypoglycaemia during Ramadan and at the end of the study. During Ramadan, the number of participants reporting hypoglycaemia was significantly lower in the telemonitoring group [Odds ratio (OR): 0.186, 95% confidence interval: 0.04–0.936; p = 0.04]. Similarly, the proportion of participants reporting symptomatic hypoglycaemia at the end of the study was significantly lower in the telemonitoring group (OR: 0.257, 95% CI: 0.07–0.89; p = 0.03). A reduction of 1.07% in glycated haemoglobin levels was observed in the telemonitoring group compared to 0.24% in the control group (p < 0.01). Overall, telemonitoring was a useful adjunct to reduce the risk of hypoglycaemia during Ramadan with no deterioration in glycaemic control
Free-Space Nonlinear Beam Combining for High Intensity Projection
Authors: Rostami Fairchild Shermineh , Walasik Wiktor , Kepler Daniel , Baudelet Matthieu , Litchinitser Natalia M. , Richardson Martin 
Published: August 2017

AbstractThe controlled interaction of two high intensity beams opens new degrees of freedom for manipulating electromagnetic waves in air. The growing number of applications for laser filaments requires fine control of their formation and propagation. We demonstrate, experimentally and theoretically, that the attraction and fusion of two parallel ultrashort beams with initial powers below the critical value (70% Pcritical), in the regime where the non-linear optical characteristics of the medium become dominant, enable the eventual formation of a filament downstream. Filament formation is delayed to a predetermined distance in space, defined by the initial separation between the centroids, while still enabling filaments with controllable properties as if formed from a single above-critical power beam. This is confirmed by experimental and theoretical evidence of filament formation such as the individual beam profiles and the supercontinuum emission spectra associated with this interaction.
Activation of the peripheral immune system regulates neuronal aromatase in the adult zebra finch brain
Authors: Pedersen Alyssa L. , Gould Cassie J. , Saldanha Colin J. 
Published: August 2017

AbstractEstradiol provision via neural aromatization decreases neuro-inflammation and –degeneration, but almost nothing is known about the interactions between the peripheral immune system and brain aromatase. Given the vulnerability of the CNS we reasoned that brain aromatization may protect circuits from the threats of peripheral infection; perhaps shielding cells that are less resilient from the degeneration associated with peripheral infection or trauma. Lipopolysaccharide (LPS) or vehicle was administered peripherally to adult zebra finches and sickness behavior was recorded 2 or 24 hours later. The central transcription of cytokines and aromatase was measured, as were telencephalic aromatase activity and immunoreactive aromatase (24 hour time point only). Two hours post LPS, sickness-like behaviors increased, the transcription of IL-1β was higher in both sexes, and TNFα was elevated in females. 24 hours post-LPS, the behavior of LPS birds was similar to controls, and cytokines had returned to baseline, but aromatase mRNA and activity were elevated in both sexes. Immunocytochemistry revealed greater numbers of aromatase-expressing neurons in LPS birds. These data suggest that the activation of the immune system via peripheral endotoxin increases neuronal aromatase; a mechanism that may rapidly generate a potent anti-neuroinflammatory steroid in response to peripheral activation of the immune system.
Condensation Mechanism of Hydrocarbon Field Formation
Authors: Batalin Oleg , Vafina Nailya 
Published: August 2017

AbstractPetroleum geology explains how hydrocarbon fluids are generated, but there is a lack of understanding regarding how oil is expelled from source rocks and migrates to a reservoir. To clarify the process, the multi-layer Urengoy field in Western Siberia was investigated. Based on this example, we have identified an alternative mechanism of hydrocarbon field formation, in which oil and gas accumulations result from the phase separation of an upward hydrocarbon flow. There is evidence that the flow is generated by the gases released by secondary kerogen destruction. This study demonstrates that oil components are carried by the gas flow and that when the flow reaches a low-pressure zone, it condenses into a liquid with real oil properties. The transportation of oil components in the gas flow provides a natural explanation for the unresolved issues of petroleum geology concerning the migration process. The condensation mechanism can be considered as the main process of oil field formation.
SIRT1 rs10823108 and FOXO1 rs17446614 responsible for genetic susceptibility to diabetic nephropathy
Authors: Zhao Yanyan , Wei Junfang , Hou Xuefeng , Liu Huimiao , Guo Feng , Zhou Yingni , Zhang Yuanyuan , Qu Yunhui , Gu Junfei , Zhou Yuanli , Jia Xiaobin , Qin Guijun , Feng Liang 
Published: August 2017

AbstractSIRT1 and FOXO1 play an important role in the pathogenesis of diabetic nephropathy (DN). However, the association between genetic polymorphisms and susceptibility to type 2 DN (T2DN) has not been explored. In this study, a total of 1066 patients with type 2 diabetes mellitus (T2DM) (413 without and 653 with DN) were enrolled. The genotypes of three htSNPs (rs3818292, rs4746720, rs10823108) within SIRT1 and two htSNPs (rs2721068, rs17446614) in FOXO1 were determined by PCR-RFLP. HbA1C, LDL, HDL, TC, and TG levels were also examined. SIRT1 rs10823108 AA genotype was significantly associated with a decreased risk of DN (OR = 0.60, 95%CI: 0.38–0.97), while GA genotype (OR = 1.77, 95%CI: 1.33–2.35) and AA genotype (OR = 2.32, 95%CI: 1.25–4.34) of FOXO1 rs17446614 was associated with an increased T2DN risk. The interactions among rs1744 6614, BMI and duration of diabetes (OR: 2.63, 95%CI: 1.23–4.31) were also observed. Subsequent haplotype analysis revealed that two haplotype defined by AC (OR: 1.50, 95%CI: 1.15–1.94) and AT (OR: 1.79, 95%CI: 1.06–2.80) within FOXO1 gene may increase the risk of T2DN. In conclusion, genetic variant rs10823108 in SIRT1 and variant rs17446614 in FoxO1 may contribute to the risk of DN in T2DM patients.
Indirect immobilized Jagged1 suppresses cell cycle progression and induces odonto/osteogenic differentiation in human dental pulp cells
Authors: Manokawinchoke Jeeranan , Nattasit Praphawi , Thongngam Tanutchaporn , Pavasant Prasit , Tompkins Kevin A. , Egusa Hiroshi , Osathanon Thanaphum 
Published: August 2017

AbstractNotch signaling regulates diverse biological processes in dental pulp tissue. The present study investigated the response of human dental pulp cells (hDPs) to the indirect immobilized Notch ligand Jagged1 in vitro. The indirect immobilized Jagged1 effectively activated Notch signaling in hDPs as confirmed by the upregulation of HES1 and HEY1 expression. Differential gene expression profiling using an RNA sequencing technique revealed that the indirect immobilized Jagged1 upregulated genes were mainly involved in extracellular matrix organization, disease, and signal transduction. Downregulated genes predominantly participated in the cell cycle, DNA replication, and DNA repair. Indirect immobilized Jagged1 significantly reduced cell proliferation, colony forming unit ability, and the number of cells in S phase. Jagged1 treated hDPs exhibited significantly higher ALP enzymatic activity, osteogenic marker gene expression, and mineralization compared with control. Pretreatment with a γ-secretase inhibitor attenuated the Jagged1-induced ALP activity and mineral deposition. NOTCH2 shRNA reduced the Jagged1-induced osteogenic marker gene expression, ALP enzymatic activity, and mineral deposition. In conclusion, indirect immobilized Jagged1 suppresses cell cycle progression and induces the odonto/osteogenic differentiation of hDPs via the canonical Notch signaling pathway.
A membrane-inserted structural model of the yeast mitofusin Fzo1
Authors: De Vecchis Dario , Cavellini Laetitia , Baaden Marc , Hénin Jérôme , Cohen Mickaël M. , Taly Antoine 
Published: August 2017

AbstractMitofusins are large transmembrane GTPases of the dynamin-related protein family, and are required for the tethering and fusion of mitochondrial outer membranes. Their full-length structures remain unknown, which is a limiting factor in the study of outer membrane fusion. We investigated the structure and dynamics of the yeast mitofusin Fzo1 through a hybrid computational and experimental approach, combining molecular modelling and all-atom molecular dynamics simulations in a lipid bilayer with site-directed mutagenesis and in vivo functional assays. The predicted architecture of Fzo1 improves upon the current domain annotation, with a precise description of the helical spans linked by flexible hinges, which are likely of functional significance. In vivo site-directed mutagenesis validates salient aspects of this model, notably, the long-distance contacts and residues participating in hinges. GDP is predicted to interact with Fzo1 through the G1 and G4 motifs of the GTPase domain. The model reveals structural determinants critical for protein function, including regions that may be involved in GTPase domain-dependent rearrangements.
Age-related processing delay reveals cause of apparent sensory excitability following auditory stimulation
Authors: Volosin Márta , Gaál Zsófia Anna , Horváth János 
Published: August 2017

AbstractWhen background auditory events lead to enhanced auditory event-related potentials (ERPs) for closely following sounds, this is generally interpreted as a transient increase in the responsiveness of the auditory system. We measured ERPs elicited by irrelevant probes (gaps in a continuous tone) at several time-points following rare auditory events (pitch glides) in younger and older adults, who watched movies during stimulation. Fitting previous results, in younger adults, gaps elicited increasing N1 auditory ERPs with decreasing glide-gap separation. N1 increase was paralleled by an ERP decrease in the P2 interval. In older adults, only a glide-gap separation dependent P2 decrease, but no N1-effect was observable. This ERP pattern was likely caused by a fronto-central negative waveform, which was delayed in the older adult group, thus overlapping N1 and P2 in the younger, but overlapping only P2 in the older adult group. Because the waveform exhibited a polarity reversal at the mastoids, it was identified as a mismatch negativity (MMN). This interpretation also fits previous studies showing that gap-related MMN is delayed in older adults, reflecting an age-related deterioration of fine temporal auditory resolution. These results provide a plausible alternative explanation for the ERP enhancement for sounds following background auditory events.
Understanding stability diagram of perpendicular magnetic tunnel junctions
Authors: Skowroński Witold , Czapkiewicz Maciej , Ziętek Sławomir , Chęciński Jakub , Frankowski Marek , Rzeszut Piotr , Wrona Jerzy 
Published: August 2017

AbstractPerpendicular magnetic tunnel junctions (MTJ) with a bottom pinned reference layer and a composite free layer (FL) are investigated. Different thicknesses of the FL were tested to obtain an optimal balance between tunneling magnetoresistance (TMR) ratio and perpendicular magnetic anisotropy. After annealing at 400 °C, the TMR ratio for 1.5 nm thick CoFeB sublayer reached 180% at room temperature and 280% at 20 K with an MgO tunnel barrier thickness corresponding to the resistance area product RA = 10 Ohmμm2. The voltage vs. magnetic field stability diagrams measured in pillar-shaped MTJs with 130 nm diameter indicate the competition between spin transfer torque (STT), voltage controlled magnetic anisotropy (VCMA) and temperature effects in the switching process. An extended stability phase diagram model that takes into account all three effects and the effective damping measured independently using broadband ferromagnetic resonance technique enabled the determination of both STT and VCMA coefficients that are responsible for the FL magnetization switching.
Insect-bacteria parallel evolution in multiple-co-obligate-aphid association: a case in Lachninae (Hemiptera: Aphididae)
Authors: Chen Rui , Wang Zhe , Chen Jing , Jiang Li-Yun , Qiao Ge-Xia 
Published: August 2017

AbstractParallel phylogenies between aphid and its obligate symbiontBuchneraare hot topics which always focused on aphid lower taxonomic levels. Symbionts in the subfamily Lachninae are special.Buchnerain many lachnine species has undergone functional and genome size reduction that was replaced by other co-obligate symbionts. In this study, we constructed the phylogenetic relationships of Lachninae with a combined dataset of five genes sequenced fromBuchnerato estimate the effects of a dual symbiotic system in the aphid-Buchneracospeciation association. The phylogeny ofBuchnerain Lachninae was well-resolved in the combined dataset. Each of the genera formed strongly supported monophyletic groups, with the exception of the genusCinara. The phylogeny based on sequences fromBuchnerawas divided into five tribes according to the clades of the Lachninae hosts tree, with the phylogenies ofBuchneraand Lachninae being generally congruent. These results first provided evidence of parallel evolution at the aphid subfamily level comprehensively and supported the view that topological congruence between the phylogenies ofBuchneraand Lachninae would not be interfered with the other co-obligate symbionts, such asSarretia, in aphid-entosymbiont association. These results also provided new insight in understanding host-plant coevolution in lachnine lineages.
Stress buildup and drop in inland shallow crust caused by the 2011 Tohoku-oki earthquake events
Authors: Sakaguchi Kiyotoshi , Yokoyama Tatsuya , Lin Weiren , Watanabe Noriaki 
Published: August 2017

AbstractTo examine the change in stress between before and after the Tohoku-oki Mw9.0 earthquake, we performed stress measurements after the earthquake in the Kamaishi mine in Iwate prefecture in northern Japan, located near the northern termination of the mainshock rupture, following previous measurements before the earthquake in the same mine. The results showed that the magnitudes of the three-dimensional principal stresses and the vertical stress drastically increased after the mainshock and, at 1 year after the earthquake, were more than double those before the earthquake. The principal stress magnitudes then decreased with time and returned to almost pre-earthquake levels at about 3 years after the earthquake. These changes can be interpreted in terms of coseismic rupture of the mainshock and the occurrence of aftershocks in the Sanriku-oki low-seismicity region (SLSR), where the Kamaishi mine is located. The drastic increase in stress suggests that the SLSR may act as a barrier to further rupture propagation.
Long intergenic non-coding RNA GALMD3 in chicken Marek’s disease
Authors: Han Bo , He Yanghua , Zhang Li , Ding Yi , Lian Ling , Zhao Chunfang , Song Jiuzhou , Yang Ning 
Published: August 2017

AbstractLong intergenic non-coding RNAs (lincRNAs) are transcribed from non-coding DNA sequences. Studies have revealed that aberrant expressions of lincRNAs are associated with various types of cancers and neurological disorders. Marek’s disease (MD) is a highly contagious T-cell lymphoid neoplasia of chicken induced by Marek’s disease virus (MDV). In this study, we first identified and validated linc-GALMD3 highly expressed in MDV-infected CD4+ T cells by RNA-Seq and qRT-PCR. By RNA-Seq analysis in MDCC-MSB1 cells after loss of function of linc-GALMD3 by shRNA, we found that linc-GALMD3 could positively cis-regulate its downstream gga-miR-223 gene expression. In contrast, it could trans-regulate the 748 differentially expressed genes (FDR < 0.01) that were mainly enriched into mitochondrial structure and cell cycle processes using GO analysis. Of these, the most significantly expressed gene EPYC might cause iris lesion in MD. The other eight genes, NDUFA4, NDUFB6, NDUFV1, NDUFS8, SDHB, UQCRC1, UQCRC2, and COX7A2, actively participated in oxidative phosphorylation in mitochondrial dysfunction and cell death. Most importantly, we found that the MDV replication was repressed when linc-GALMD3 was knocked down in CEF cells. Our results suggested that linc-GALMD3 might be a critical regulator in chicken MD and could be used as a candidate-promising mark for MD prevention, diagnosis, and treatment.
1α,20S-Dihydroxyvitamin D3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis
Authors: Lin Zongtao , Chen Hao , Belorusova Anna Y. , Bollinger John C. , Tang Edith K. Y. , Janjetovic Zorica , Kim Tae-Kang , Wu Zhongzhi , Miller Duane D. , Slominski Andrzej T. , Postlethwaite Arnold E. , Tuckey Robert C. , Rochel Natacha , Li Wei 
Published: August 2017

Abstract1α,20S-Dihydroxyvitamin D3 [1,20S(OH)2D3], a natural and bioactive vitamin D3 metabolite, was chemically synthesized for the first time. X-ray crystallography analysis of intermediate 15 confirmed its 1α-OH configuration. 1,20S(OH)2D3 interacts with the vitamin D receptor (VDR), with similar potency to its native ligand, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] as illustrated by its ability to stimulate translocation of the VDR to the nucleus, stimulate VDRE-reporter activity, regulate VDR downstream genes (VDR, CYP24A1, TRPV6 and CYP27B1), and inhibit the production of inflammatory markers (IFNγ and IL1β). However, their co-crystal structures revealed differential molecular interactions of the 20S-OH moiety and the 25-OH moiety to the VDR, which may explain some differences in their biological activities. Furthermore, this study provides a synthetic route for the synthesis of 1,20S(OH)2D3 using the intermediate 1α,3β-diacetoxypregn-5-en-20-one (3), and provides a molecular and biological basis for the development of 1,20S(OH)2D3 and its analogs as potential therapeutic agents.