bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of genetic diversity of Brassica rapa L. accessions from the VIR collection by analyzing DNA polymorphism in microsatellite loci
Authors: Berensen F. A. 
Published: September 2024

The species Brassica rapa L. includes leafy and root crops grown in many countries of the world. Clarification of genetic relationships and population structure allows for a more accurate parental line selection for further breeding using genetically distinct forms. The B. rapa collection maintained at VIR contains 1750 accessions representing different subspecies and countries of origin, and it is a good tool for studying the diversity of cultivated forms using molecular genetic methods. The aim of the study was to investigate the genetic diversity and clarify the relationships within the B. rapa species using molecular markers. Molecular screening of 80 accessions using 16 microsatellite markers revealed alleles ranging in size from 85 to 460 bp with an average of 7.8 alleles per locus. The average polymorphic index content (PIC) was 0.278, and the expected heterozygosity (H) averaged was 0.35. Rare and unique alleles were identified for Beijing cabbage (k-63 and k-108), Japanese leafy vegetables (k-217 and k-335) and turnip (k-738) samples. Alleles of SSR loci BRMS-007 (123 bp) and BRMS-034 (136 bp) were identified, which are characteristic only of accessions of turnips, rapeseed and sarsons. In silico analysis of primer pairs was performed to clarify the sizes of expected fragments relative to the reference genome CAAS_Brap_v3.01 of Chinese cabbage line Chiifu-401-42. Phylogenetic analysis was performed using the STRUCTURE program and resulted in the distribution of accessions into four clusters according to botanical classification: Chinese cabbage; pakchoi, tatsoi; Japanese leafy vegetables, wutacai, tsoisum, mizuna and mibuna (kyona) and hybrid forms; turnip and rape accessions.
Penerapan Media Komik untuk Meningkatkan Kemampuan Membaca Pemula Siswa Sekolah Dasar
Authors: Irnawati Irnawati , Uswatun Din Azwar , Nurmeta Irna Khaleda 
Published: September 2024

Kemampuan membaca merupakan fondasi krusial bagi pelajar tingkat dasar, dan komik kini diakui sebagai media yang dapat membantu untuk mengembangkan keterampilan ini. Penelitian ini bertujuan untuk mendeskripsikan keterlasaan media komik dan mendeskripsikan peningkatan kemampuan membaca siswa sekolah dasar. Metode yang diterapkan adalah penelitian tindakan kelas dengan dua tahapan siklus, mencakup fase perencanaan, tindakan, pengamatan, dan Refleksi. Temuan penelitian mengindikasikan adanya progres dalam kemampuan membaca pemula siswa di SDN Cilengo melalui penggunaan media komik. Pada siklus pertama, 50% siswa (16 dari 32) berhasil melampaui nilai KKM dengan rerata 71,91. Siklus kedua menunjukkan peningkatan substansial dimana 87,5% siswa (28 dari 32) mencapai nilai di atas KKM dengan rerata 83,44. Pengamatan terhadap aktivitas guru dan siswa juga menunjukkan perbaikan dari siklus I ke siklus II. Bahwa media komik dapat meningkatkan kemampuan membaca pemula siswa sekolah dasar
Analysis of Mustard Oil Value Chain in Province 2 of Nepal: Evidence from Logistic Regression
Authors: Shah Sanjeet , Devkota Niranjan 
Published: September 2024

Background: Mustard oil is used by millions of People in south Asian region due to its health benefit, livelihood and employment opportunities. For smooth operation from growing to reach at customers hand value chain supports a lot. It is not only helping farmers but also generating opportunities in each stage. Thus, this study aims to identify value chain analysis of mustard oil in Nepal.
Objective: Aim of this study is to identify the on-Value Chain Analysis of Mustard Oil.
Method: The paper followed the design of explanatory research and collected data using structured questionnaires. Binary Logistic Model was used for inferential analysis. Siraha and Saptari Districts are chosen purposely. 164 respondents were conveniently sampled from farmers and traders.
Result: The study shows the cooperatives play role in accessing credit, accessing input and training with the help of government and non- government organizations. The study tells us the fact that the mustard value chain functions in a traditional way, there is no innovative way of integrating the chain actors. Though the cooperatives are involved in both input supply and in credit services, their role is not as expected. Farmers are not getting adequate advisory service from cooperatives. Ages, off-farm income, access to information, access to input are the factors that add value in mustard value chain.
Conclusion: The study concluded that the value chain is currently underdeveloped; there is no innovative way of integrating the chain actors in value chain. Farmers are not getting adequate advisory service from cooperatives.
Paper Types: Research Paper
Keywords: Value Chain Analysis, Farmer, Traders, Value addition, Logistic Model, Kobo toolbox.
JEL Classification: N55, Q8, Q18 and Q2
Il coltello dello sguardo
Authors: Cortellessa Andrea 
Published: September 2024

Un’analisi del libro pubblicato da Antonella Anedda nel 2009, La vita dei dettagli, esemplare unico di collezione di testi in varia misura accomunati dal rapporto con le immagini della storia dell’arte, consente di ricostruire diversi temi e genealogie che anche nel resto del corpus dell’autrice s’incentrano sullo sguardo, l’attenzione, il senso dell’intimità e della caducità.

English titile: «The Eye as a Knife»
An analysis of the book published by Antonella Anedda in 2009, La vita dei dettagli, a unique collection of texts to varying degrees united by the relationship with images of art history, allows us to reconstruct different themes and genealogies that also in the rest of the author's corpus focus on gaze, attention, sense of intimacy and transience.
PENGARUH TEKNOLOGI INFORMASI, KEMAMPUAN PEMAKAI SISTEM INFORMASI AKUNTANSI DAN PENGALAMAN KERJA TERHADAP KINERJA KARYAWAN BAGIAN AKUNTANSI
Authors: Irwansyah Irwansyah 
Published: September 2024

Penelitian ini bertujuan untuk membuktikan pengaruh teknologi informasi, kemampuan pemakai sistem informasi akuntansi, dan pengalaman kerja terhadap kinerja karyawan bagian akuntansi. Penelitian ini menggunakan pendekatan kuantitatif. Responden dalam penelitian ini adalah 41 karyawan bagian akuntansi pada perusahaan otomotif di Kota Bengkulu. Penelitian ini mengumpulkan data primer dengan kuesioner. Pemilihan sampel yang diuji dalam penelitian ini menggunakan purposive sampling. Analisis yang digunakan adalah Statistical Package for the Social Sciences (SPSS).Hasil penelitian menunjukkan teknologi informasi berpengaruh positif terhadap kinerja karyawan bagian akuntansi, kemampuan pemakai sistem informasi akuntansi berpengaruh positif terhadap kinerja karyawan bagian akuntansi, dan pengalaman kerja berpengaruh positif terhadap kinerja karyawan bagian akuntansi.
Modulation of left ventricular hypertrophy in spontaneously hypertensive rats by acetylcholinesterase and ACE inhibitors: physiological, biochemical, and proteomic studies
Authors: Hejnova Lucie , Drastichova Zdenka , Boroš Almos , Hrdlicka Jaroslav , Behuliak Michal , Neckar Jan , Zicha Josef , Novotny Jiri 
Published: September 2024

BackgroundThe consequences at the molecular level and the mechanisms involved in a possible cardioprotective effect of antihypertensive treatment are not yet fully understood. Here, the efficacy of pyridostigmine (PYR) and trandolapril (TRA) as antihypertensive and antihypertrophic agents was investigated and compared in hypertensive SHR and normotensive WKY rats. In parallel, we investigated the effects of these drugs on myocardial β-adrenergic and cholinergic signaling pathways and protein expression profiles.MethodsAge-matched male SHR and WKY rats were chronically (8 weeks) treated with PYR or TRA in drinking water. Blood pressure (BP) and heart rate (HR) were monitored telemetrically prior to tissue sampling for biochemical analysis. Baroreceptor reflex sensitivity (BRS) and methylatropine HR response as a measure of vagal tone were evaluated in separate groups of animals.ResultsPYR slightly lowered BP and HR in SHR rats during the dark phase of the day, while TRA effectively reduced BP during the light and dark phases without affecting HR. PYR enhanced BRS and improved vagal tone. There were no significant alterations in myocardial β-adrenergic and cholinergic signaling, with the exception of decreased forskolin-stimulated adenylyl cyclase (AC) activity in SHR rats, which was restored by TRA. Proteomic analysis revealed numerous differences induced by both treatments. Notable were changes in TGFβ-related signaling pathways as well as proteins involved in modifying hemodynamic parameters and cardiac hypertrophy.ConclusionsPYR is able to slightly decrease BP and HR in SHR rats but effectively increase BRS through vagal potentiation. The specific differences in protein expression profiles in rat myocardium induced by treatment with PYR and TRA reflect different mechanisms of action of these two agents at the molecular level.
Electrospun patches to deliver combination drug therapy for fungal infections
Authors: Dziemidowicz Karolina , Meszarik Mark , Piovesan Jacopo , Almatroudi Mazna A. , Williams Gareth R. , Murdan Sudaxshina 
Published: September 2024

Fungal infections, though affecting healthcare globally, receive insufficient attention in clinical and academic settings. Invasive fungal infections, particularly caused by combat wounds, have been identified as a critical threat by the US Department of Defense. Monotherapy with traditional antifungals is often insufficient, and so combination therapies are explored to enhance treatment efficacy. However, systemic combination treatments can result in severe adverse effects, suggesting the need for localised delivery systems, such as drug-loaded electrospun patches, to administer antifungals directly to the infection site. This proof-of-concept study hypothesised that dual amorolfine and terbinafine therapy slowly releasing from electrospun patches would be an effective way of eradicating Candida albicans when the patch was applied directly to the fungal colony. The feasibility of creating electrospun materials loaded with amorolfine and terbinafine for combination antifungal therapy was investigated. Electrospinning was used to fabricate polycaprolactone (PCL) patches with varying drug loadings (2.5%, 5%, and 10% w/w) of amorolfine and terbinafine either individually or in combination. The incorporation of both drugs in the fibres was confirmed, with the drugs predominantly in an amorphous state. Results showed that combination therapy patches had a significantly greater and prolonged antifungal effect compared to monotherapy patches, with larger zones of inhibition and sustained efficacy over at least 7 days. This study therefore demonstrates that PCL-based electrospun patches containing amorolfine and terbinafine provide superior antifungal activity against C. albicans compared to monotherapy patches. This approach could lower required drug doses, reducing adverse effects, and enhance patient compliance due to prolonged drug release, leading to more effective antifungal therapy.
Glycated CD59 is a potential biomarker for gestational diabetes mellitus
Authors: Wang Wanying , Xu Chong , Lu Xiaofan , Cao Wei , Zuo Tengzi , Zhang Ying , Zou Huiling , Sun Yu 
Published: September 2024

ObjectiveTo explore the diagnostic value of glycated CD59 (gCD59) in gestational diabetes mellitus (GDM).MethodsA total of 707 pregnant women who underwent the first visit in the obstetric outpatient clinic of the Affliated Suqian Hospital of Xuzhou Medical University from January 2022 to July 2023 were included, and were grouped according to the International Association of the Diabetes and Pregnancy Study Groups(IADPSG) diagnostic criteria, and finally 113 cases in the GDM group and 559 cases in the normal glucose tolerance (NGT) group were included, and the concentration of gCD59 was determined by enzyme-linked immunosorbent assay (ELISA). The baseline data characteristics of the two groups were compared, the risk factors for GDM were explored by multivariate binary logistic analysis, and the diagnostic value of gCD59 in predicting GDM was explored by receiver operating characteristic (ROC) curve analysis.ResultsThe level of gCD59 in the GDM group was significantly higher than that in the NGT group (1.49 SPU vs 0.87 SPU). Multivariate regression analysis showed that gCD59, diastolic blood pressure (DBP) and thyroid stimulating hormone (TSH) were independent risk factors for GDM.The area under the curve (AUC) of gCD59 for the diagnosis of GDM was 0.681 (95% CI: 0.583-0.717), with a sensitivity of 71.7% and a specificity of 58.3%. In combination with fasting glucose, gCD59 effectively diagnosed GDM with higher AUC of 0.871 (95% CI: 0.708-1.000).ConclusiongCD59 is an independent risk factor for GDM and a good biomarker for the diagnosis of GDM.
Recurrent laryngeal nerve monitoring by flexible laryngoscopy during thyroid radiofrequency ablation in the awake patient
Authors: Teliti Marsida , Occhini Antonio , Fonte Rodolfo , Croce Laura , Calì Benedetto , Ripepi Federica Antonella , Carbone Andrea , Rotondi Mario , Chytiris Spyridon 
Published: September 2024

ObjectiveAlthough radiofrequency ablation (RFA) is a safe and effective non-surgical treatment for benign thyroid nodules, injury to the recurrent laryngeal nerve (RLN), is a potential and feared complication. Intermittent voice checks have been proposed to monitor vocal cord (VC) function during RFA, but such assessment is highly subjective and effort-dependent.MethodsWe are here reporting the methodological use of flexible laryngoscopy (FL) for VC monitoring during bilateral thyroid RFA treatment. The patient, a 35-year-old woman, was referred to the Endocrinology Unit for subclinical hyperthyroidism due to bilateral autonomously functioning thyroid nodules.ResultsAt the end of the treatment of the first nodule, the FL performed by an otorhinolaryngologist specialist allowed evaluating VC function and ruling out possible paralysis before proceeding with the contralateral RFA treatment. The patient was awake during the entire procedure and well tolerated the laryngoscopic examination. The TSH serum evaluations performed one month and 9 months after the procedure assessed an euthyroid state (TSH 3.2 mIU/L and 2.8 mIU/L, respectively).ConclusionDuring bilateral thyroid RFA the use of FL for VC monitoring treatment resulted in a safe, easy-to-perform, and effective strategy to minimize and anticipate RLN injury risk in the awake patient. The prevention of RLN damage is advisable in the case of single RFA treatment, while it should be strongly recommended when RFA is performed on bilateral nodules.
Gender-specific correlations between remnant cholesterol and severe abdominal aortic calcification in American adults
Authors: Yan Laisha , Hu Xiaoyan , Wu Shanshan , Zhao Shunying 
Published: September 2024

BackgroundRemnant cholesterol (RC) predicts cardiovascular risk and is associated with a range of diseases, including asthma, hypertension, depression, periodontitis, and alcoholic fatty liver disease. However, its correlation with abdominal aortic calcification (AAC) has not been reported.MethodsUsing a cross-sectional approach, this study examined data from the 2013-2014 National Health and Nutrition Examination Survey (NHANES) cycle. Multiple logistic regression, generalized summation models, and subgroup analyses were used in examining the correlation between RC and the prevalence of severe AAC.ResultsThe mean age of participants in this study was 57.70 ± 11.73 years, with 142 individuals (9.67%) suffering from severe AAC. The median RC was 0.52 mmol/L (Q1-Q3, 0.36-0.75 mmol/L). Among female participants, a significant positive correlation was observed between RC and severe AAC (per natural log [RC] increment: 2.14; 95% CI, 1.07-4.27). Smooth curve fitting and threshold effect analysis revealed a saturation effect at an RC level of 0.57 mmol/L. Conversely, in male participants, no significant correlation was found between RC and the prevalence of severe AAC (per natural log [RC] increment: 0.88; 95% CI, 0.43-1.78). Our findings suggest a significant interaction between gender and RC in relation to severe AAC (P for interaction = 0.0042).ConclusionsHigher RC levels were significantly associated with an increased prevalence of severe AAC in women.
Integration of smart charging of large-scale electric vehicles into generation and storage expansion planning: a case study in south china
Authors: You Lei , Jin Xiaoming , Liu Yun 
Published: September 2024

This paper studies how to integrate the smart charging of large-scale electric vehicles (EVs) into the generation and storage expansion planning (GSEP), while analyzing the impact of smart charging on the GSEP of a real power system in south China. For this purpose, a random simulation-based method is first developed to provide the tractable formulations of the adjustable charging load and reserve provision from EVs. This method avoids the unrealistic assumption that EVs drive and charge every day, which often exists in prior relevant approaches. Based on the random simulation, this paper proposes a novel GSEP optimization model which incorporates the weekly adjustable charging load of EVs. In the proposed model, the total charging load of EVs can be co-optimized with the investment and operational decisions of various generation and storage units. This GSEP model is applied to a provincial power system in south China. The numerical results show that the implementation of smart charging can significantly alter the decisions of GSEP. As the participation rate of smart charging improves from 0% to 90%, there is an additional 1,800 MW installation in wind and solar power, while the need to build new batteries is noticeably reduced; also, depending on the level of EV uptake, the annualized total system cost decreases by 5.11%–7.57%, and the curtailment of wind and solar power is reduced by 10.34%–19.64%. Besides, numerical tests reveal that the traditional assumption that EVs drive and charge every day can mislead the evaluation of adjustable charging load and overestimate the daily charging power peak by averagely 24.72%.
Berberine alleviates ETEC-induced intestinal inflammation and oxidative stress damage by optimizing intestinal microbial composition in a weaned piglet model
Authors: Wang Yue , Zhang Ziting , Du Min , Ji Xu , Liu Xiaodan , Zhao Chunfang , Pang Xunsheng , Jin Erhui , Wen Aiyou , Li Shenghe , Zhang Feng 
Published: September 2024

IntroductionEnterotoxigenic Escherichia coli (ETEC) is the main diarrhea-causing pathogen in children and young animals and has become a global health concern. Berberine is a type of “medicine and food homology” and has a long history of use in China, particularly in treating gastrointestinal disorders and bacterial diarrhea.MethodsIn this study, we explored the effects of berberine on growth performance, intestinal inflammation, oxidative damage, and intestinal microbiota in a weaned piglet model of ETEC infection. Twenty-four piglets were randomly divided into four groups—a control group (fed a basal diet [BD] and infused with saline), a BD+ETEC group (fed a basal diet and infused with ETEC), a LB+ETEC group (fed a basal diet with 0.05% berberine and infused with ETEC infection), and a HB+ETEC group (fed a basal diet with 0.1% berberine and infused with ETEC).ResultsBerberine significantly improved the final body weight (BW), average daily gain (ADG), and average daily feed intake (ADFI) (P&lt;0.05) of piglets, and effectively decreased the incidence of diarrhea among the animals (P&lt;0.05). Additionally, berberine significantly downregulated the expression levels of the genes encoding TNF-α, IL-1β, IL-6, IL-8, TLR4, MyD88, NF-κB, IKKα, and IKKβ in the small intestine of piglets (P&lt;0.05). ETEC infection significantly upregulated the expression of genes coding for Nrf2, CAT, SOD1, GPX1, GST, NQO1, HO-1, GCLC, and GCLM in the small intestine of the animals (P&lt;0.05). Berberine significantly upregulated 12 functional COG categories and 7 KEGG signaling pathways. A correlation analysis showed that berberine significantly increased the relative abundance of beneficial bacteria (Gemmiger, Pediococcus, Levilactobacillus, Clostridium, Lactiplantibacillus, Weissella, Enterococcus, Blautia, and Butyricicoccus) and decreased that of pathogenic bacteria (Prevotella, Streptococcus, Parabacteroides, Flavonifractor, Alloprevotella) known to be closely related to intestinal inflammation and oxidative stress in piglets. In conclusion, ETEC infection disrupted the intestinal microbiota in weaned piglets, upregulating the TLR4/MyD88/NF-κB and Nrf2 signaling pathways, and consequently leading to intestinal inflammation and oxidative stress-induced damage.DiscussionOur data indicated that berberine can optimize intestinal microbiota balance and modulate the TLR4/MyD88/NF-κB and Nrf2 signaling pathways, thus helping to alleviate intestinal inflammation and oxidative damage caused by ETEC infection in weaned piglets.
Educational priorities for primary care providers on systemic lupus erythematosus in the Caribbean
Authors: Lawrence Jannel , King Amanda , Ramessar Nina , Henry Danielle , Davis Stacy , Davis-King Keisha , McGhie Taniesha , Rolle Anishka , Manno Rebecca , Dowell Sharon 
Published: September 2024

IntroductionSystemic lupus erythematosus (SLE) is highly prevalent in the Caribbean, with limited access to specialist care. Primary care providers (PCPs) must play a key role in SLE management but lack specific knowledge on high-impact diagnostic and management strategies for the Caribbean SLE patient. The Caribbean Association for Rheumatology (CAR) supports PCPs with educational material tailored to the Caribbean but is limited by poor regional data on SLE educational needs. The aim of this project was to achieve consensus amongst CAR members on SLE educational priorities for PCPs based on individual experience.MethodsThe nominal group technique was used to achieve consensus. Participants were asked to generate, record, discuss and rank ideas, which were organized by emergent categories. Each participant assigned a score to their top ideas, generating a group-level score for each topic. A threshold of at least 70% agreement was set for consensus. Narrative comments from the discussion and ranking survey provided context for the consensus topics.Results13 providers (9 rheumatologists and 4 internists), mostly female (92%), mean age 42.4 (8.2) years, participated. There was consensus on 15 topics which emphasized early diagnosis, early rheumatology referral and reproductive health. Of the top-ranked topics, there was a trend towards a primary care focus amongst internists.ConclusionPriority topics for PCP education focused on early diagnosis, early referral and reproductive health in SLE. Non-rheumatologists prioritized primary care interventions reflecting a misalignment in the perception of the Caribbean PCP's role in SLE, reinforcing the need for a focused educational program.