bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advances and challenges in barcoding pathogenic and environmentalLeptospira
Authors: GUERNIER VANINA , ALLAN KATHRYN J. , GOARANT CYRILLE 
Published: July 2017

SUMMARYLeptospirosis is a zoonotic bacterial disease of global importance. A large spectrum of asymptomatic animal hosts can carry the infection and contribute to the burden of human disease. Environmental sources of human contamination also point to the importance of a hydrotelluric reservoir. Leptospirosis can be caused by as many as 15 different pathogenic or intermediateLeptospiraspecies. However, classification of these bacteria remains complicated through the use of both serological and genetic classification systems that show poor correlation. With the advent of molecular techniques, DNA-based barcoding offers a conceptual framework that can be used for leptospirosis surveillance as well as source tracking. In this review, we summarize some of the current techniques, highlight significant successes and weaknesses and point to the future opportunities and challenges to successfully establish a widely applicable barcoding scheme forLeptospira.
From Cyber Norms to Cyber Rules: Re-engaging States as Law-makers
Authors: MAČÁK KUBO 
Published: July 2017

AbstractSeveral indicators point to a crisis at the heart of the emerging area of international cyber security law. First, proposals for binding international treaties by leading stakeholders, including China and Russia, have been met with little enthusiasm by other states, and are generally seen as having limited prospects of success. Second, states are extremely reluctant to commit themselves to specific interpretations of controversial legal questions and thus to express their cyberopinio juris. Third, instead of interpreting or developing rules, state representatives seek refuge in the more ambiguous term ‘norms’. This article argues that the reluctance of states to engage in international law-making has left a power vacuum, lending credence to claims that international law fails in addressing modern challenges posed by rapid technological development. In response, several non-state-driven norm-making initiatives have sought to fill the void, including Microsoft's cyber norms proposals and theTallinn Manualproject. The article then contends that this emerging body of non-binding norms presents states with a critical window of opportunity to reclaim a central law-making position, similar to historical precedents including the development of legal regimes for Antarctica and nuclear safety. Whether the supposed crisis will lead to the demise of inter-state cyberspace governance or a recalibration of legal approaches will thus be decided in the near future. States should assume a central role if they want to ensure that the existing power vacuum is not exploited in a way that would upset their ability to achieve strategic and political goals.
Could photosynthesis function on Proxima Centauri b?
Authors: Ritchie Raymond J. , Larkum Anthony W.D. , Ribas Ignasi 
Published: July 2017

AbstractCould oxygenic and/or anoxygenic photosynthesis exist on planet Proxima Centauri b? Proxima Centauri (spectral type – M5.5 V, 3050 K) is a red dwarf, whereas the Sun is type G2 V (5780 K). The light regimes on Earth and Proxima Centauri b are compared with estimates of the planet's suitability for Chlorophylla(Chla) and Chld-based oxygenic photosynthesis and for bacteriochlorophyll (BChl)-based anoxygenic photosynthesis. Proxima Centauri b has low irradiance in the oxygenic photosynthesis range (400–749 nm: 64–132 µmol quanta m−2s−1). Much larger amounts of light would be available for BChl-based anoxygenic photosynthesis (350–1100 nm: 724–1538 µmol quanta m−2s−1). We estimated primary production under these light regimes. We used the oxygenic algaeSynechocystisPCC6803,Prochlorothrix hollandica,Acaryochloris marina,Chlorella vulgaris,Rhodomonassp. andPhaeodactylum tricornutumand the anoxygenic photosynthetic bacteriaRhodopseudomonas palustris(BChla),Afifella marina(BChla),Thermochromatium tepidum(BChla),Chlorobaculum tepidum(BChla + c) andBlastochloris viridis(BChlb) as representative photosynthetic organisms. Proxima Centauri b has only ≈3% of the PAR (400–700 nm) of Earth irradiance, but we found that potential gross photosynthesis (Pg) on Proxima Centauri b could be surprisingly high (oxygenic photosynthesis: earth ≈0.8 gC m−2h−1; Proxima Centauri b ≈0.14 gC m−2h−1). The proportion of PAR irradiance useable by oxygenic photosynthetic organisms (the sum of Blue + Red irradiance) is similar for the Earth and Proxima Centauri b. The oxygenic photic zone would be only ≈10 m deep in water compared with ≈200 m on Earth. ThePgof an anoxic Earth (gC m−2h−1) is ≈0.34–0.59 (land) and could be as high as ≈0.29–0.44 on Proxima Centauri b. 1 m of water does not affect oxygenic or anoxygenic photosynthesis on Earth, but on Proxima Centauri b oxygenicPgis reduced by ≈50%. Effective elimination of near IR limitsPgby photosynthetic bacteria (<10% of the surface value). The spectrum of Proxima Centauri b is unfavourable for anoxygenic aquatic photosynthesis. Nevertheless, a substantial aerobic or anaerobic ecology is possible on Proxima Centauri b. Protocols to recognize the biogenic signature of anoxygenic photosynthesis are needed.
Kosovo and intersecting legal regimes: An interdisciplinary analysis
Authors: ORAKHELASHVILI ALEXANDER 
Published: July 2017

Abstract:The unilateral declaration of independence by Kosovar authorities in Pristina in 2008 has been the source of various controversies in international affairs. From a legal perspective, Kosovo’s secessionist drive is contrary to the well-established position of international law regarding the territorial integrity of states. From a political perspective, Kosovo’s case exemplifies the political drive to alter the law – a drive that applies to other entities in Kosovo’s position. Both these phenomena are accompanied by the divergent interests held by Kosovars as the ‘local agency’ and by the interests of Serbia and third states (including great powers) that support or oppose Kosovo’s independence. The interdisciplinary nature of this matter is enhanced by the intersection of applicable legal frameworks with competing political interests. The motivating factors – and implications of – great power conduct in this context should be examined through the prism of political realism, which provides an enhanced perspective on the relationship between legal and political factors in all their complexity.
Central insulin modulates food valuation via mesolimbic pathways
Authors: Tiedemann Lena J. , Schmid Sebastian M. , Hettel Judith , Giesen Katrin , Francke Paul , Büchel Christian , Brassen Stefanie 
Published: July 2017

AbstractCentral insulin is thought to act at the neural interface between metabolic and hedonic drives to eat. Here, using pharmacological fMRI, we show that intranasal insulin (INI) changes the value of food cues through modulation of mesolimbic pathways. Overnight fasted participants rated the palatability of food pictures and attractiveness of non-food items (control) after receiving INI or placebo. We report that INI reduces ratings of food palatability and value signals in mesolimbic regions in individuals with normal insulin sensitivity. Connectivity analyses reveal insulinergic inhibition of forward projections from the ventral tegmentum to the nucleus accumbens. Importantly, the strength of this modulation predicts decrease of palatability ratings, directly linking neural findings to behaviour. In insulin-resistant participants however, we observe reduced food values and aberrant central insulin action. These data demonstrate how central insulin modulates the cross-talk between homeostatic and non-homeostatic feeding systems, suggesting that dysfunctions of these neural interactions may promote metabolic disorders.
Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair
Authors: Niida Hiroyuki , Matsunuma Ryoichi , Horiguchi Ryo , Uchida Chiharu , Nakazawa Yuka , Motegi Akira , Nishimoto Koji , Sakai Satoshi , Ohhata Tatsuya , Kitagawa Kyoko , Moriwaki Shinichi , Nishitani Hideo , Ui Ayako , Ogi Tomoo , Kitagawa Masatoshi 
Published: July 2017

AbstractHBO1, a histone acetyl transferase, is a co-activator of DNA pre-replication complex formation. We recently reported that HBO1 is phosphorylated by ATM and/or ATR and binds to DDB2 after ultraviolet irradiation. Here, we show that phosphorylated HBO1 at cyclobutane pyrimidine dimer (CPD) sites mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites. Furthermore, HBO1 facilitates accumulation of SNF2H and ACF1, an ATP-dependent chromatin remodelling complex, to CPD sites. Depletion of HBO1 inhibited repair of CPDs and sensitized cells to ultraviolet irradiation. However, depletion of HBO1 in cells derived from xeroderma pigmentosum patient complementation groups, XPE, XPC and XPA, did not lead to additional sensitivity towards ultraviolet irradiation. Our findings suggest that HBO1 acts in concert with SNF2H–ACF1 to make the chromosome structure more accessible to canonical nucleotide excision repair factors.
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation
Authors: Gouge Jerome , Guthertz Nicolas , Kramm Kevin , Dergai Oleksandr , Abascal-Palacios Guillermo , Satia Karishma , Cousin Pascal , Hernandez Nouria , Grohmann Dina , Vannini Alessandro 
Published: July 2017

AbstractInitiation of gene transcription by RNA polymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-binding protein), and Bdp1. TFIIIB is required for recruitment of Pol III and to promote the transition from a closed to an open Pol III pre-initiation complex, a process dependent on the activity of the Bdp1 subunit. Here, we present a crystal structure of a Brf2–TBP–Bdp1 complex bound to DNA at 2.7 Å resolution, integrated with single-molecule FRET analysis and in vitro biochemical assays. Our study provides a structural insight on how Bdp1 is assembled into TFIIIB complexes, reveals structural and functional similarities between Bdp1 and Pol II factors TFIIA and TFIIF, and unravels essential interactions with DNA and with the upstream factor SNAPc. Furthermore, our data support the idea of a concerted mechanism involving TFIIIB and RNA polymerase III subunits for the closed to open pre-initiation complex transition.
A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure
Authors: Yao Yufeng , Lu Qiulun , Hu Zhenkun , Yu Yubin , Chen Qiuyun , Wang Qing K. 
Published: July 2017

AbstractEndoplasmic reticulum stress is an evolutionarily conserved cell stress response associated with numerous diseases, including cardiac hypertrophy and heart failure. The major endoplasmic reticulum stress signaling pathway causing cardiac hypertrophy involves endoplasmic reticulum stress sensor PERK (protein kinase-like kinase) and eIF2α-ATF4-CHOP signaling. Here, we describe a non-canonical, AGGF1-mediated regulatory system for endoplasmic reticulum stress signaling associated with increased p-eIF2α and ATF4 and decreased sXBP1 and CHOP. Specifically, we see a reduced AGGF1 level consistently associated with induction of endoplasmic reticulum stress signaling in mouse models and human patients with heart failure. Mechanistically, AGGF1 regulates endoplasmic reticulum stress signaling by inhibiting ERK1/2 activation, which reduces the level of transcriptional repressor ZEB1, leading to induced expression of miR-183-5p. miR-183-5p post-transcriptionally downregulates CHOP and inhibits endoplasmic reticulum stress-induced apoptosis. AGGF1 protein therapy and miR-183-5p regulate endoplasmic reticulum stress signaling and block endoplasmic reticulum stress-induced apoptosis, cardiac hypertrophy, and heart failure, providing an attractive paradigm for treatment of cardiac hypertrophy and heart failure.
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
Authors: Zhang Yihan , Qin Wei , Lu Xiaochan , Xu Jason , Huang Haigen , Bai Haipeng , Li Song , Lin Shuo 
Published: July 2017

AbstractPrecise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable “base editing” system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5′-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.
Electronic components embedded in a single graphene nanoribbon
Authors: Jacobse P. H. , Kimouche A. , Gebraad T. , Ervasti M. M. , Thijssen J. M. , Liljeroth P. , Swart I. 
Published: July 2017

AbstractThe use of graphene in electronic devices requires a band gap, which can be achieved by creating nanostructures such as graphene nanoribbons. A wide variety of atomically precise graphene nanoribbons can be prepared through on-surface synthesis, bringing the concept of graphene nanoribbon electronics closer to reality. For future applications it is beneficial to integrate contacts and more functionality directly into single ribbons by using heterostructures. Here, we use the on-surface synthesis approach to fabricate a metal-semiconductor junction and a tunnel barrier in a single graphene nanoribbon consisting of 5- and 7-atom wide segments. We characterize the atomic scale geometry and electronic structure by combined atomic force microscopy, scanning tunneling microscopy, and conductance measurements complemented by density functional theory and transport calculations. These junctions are relevant for developing contacts in all-graphene nanoribbon devices and creating diodes and transistors, and act as a first step toward complete electronic devices built into a single graphene nanoribbon.
Dark state with counter-rotating dissipative channels
Authors: Zhou Zheng-Yang , Chen Mi , Wu Lian-Ao , Yu Ting , You J. Q. 
Published: July 2017

AbstractDark state as a consequence of interference between different quantum states has great importance in the fields of chip-scale atomic clock and quantum information. For the Λ-type three-level system, this dark state is generally regarded as being dissipation-free because it is a superposition of two lowest states without dipole transition between them. However, previous studies are based on the rotating-wave approximation (RWA) by neglecting the counter-rotating terms in the system-environment interaction. In this work, we study non-Markovian quantum dynamics of the dark state in a Λ-type three-level system coupled to two bosonic baths and reveal the effect of counter-rotating terms on the dark state. In contrast to the dark state within the RWA, leakage of the dark state occurs even at zero temperature, as a result of these counter-rotating terms. Also, we present a method to restore the quantum coherence of the dark state by applying a leakage elimination operator to the system.
Virgin queen attraction toward males in honey bees
Authors: Bastin Florian , Cholé Hanna , Lafon Grégory , Sandoz Jean-Christophe 
Published: July 2017

AbstractAlthough the honeybee is a crucial agricultural agent and a prominent scientific model organism, crucial aspects of its reproductive behaviour are still unknown. During the mating season, honeybee males, the drones, gather in congregations 10–40 m above ground. Converging evidence suggests that drones emit a pheromone that can attract other drones, thereby increasing the size of the congregation. Virgin queens join the vicinity of the congregation after it has formed, and mate with as many as 20 males in mid-air. It is still unclear which sensory cues help virgin queens find drone congregations in the first place. Beside visual cues for long-range orientation, queens may use olfactory cues. We thus tested virgin queens’ olfactory orientation on a walking simulator in which they have full control over odour stimulation. We show that sexually-mature virgin queens are attracted to the odour bouquet from a group of living drones. They are not attracted to the bouquet from a group of workers. In addition, non-sexually receptive females (workers) of the same age are not attracted to the drone odour bouquet. Interpreted in the context of mating, these results may suggest that virgin queens use volatile olfactory cues from the drones to find the congregations.
Hybrid normal metal/ferromagnetic nanojunctions for domain wall tracking
Authors: Corte-León Héctor , Krzysteczko Patryk , Manzin Alessandra , Schumacher Hans Werner , Antonov Vladimir , Kazakova Olga 
Published: July 2017

AbstractHybrid normal metal/ferromagnetic, gold/permalloy (Au/Py), nanojunctions are used to investigate magnetoresistance effects and track magnetization spatial distribution in L-shaped Py nanostructures. Transversal and longitudinal resistances are measured and compared for both straight and 90° corner sections of the Py nanostructure. Our results demonstrate that the absolute change in resistance is larger in the case of longitudinal measurements. However, due to the small background resistance, the relative change in the transversal resistance along the straight section is several orders of magnitude larger than the analogous longitudinal variation. These results prove that hybrid nanojunctions represent a significant improvement with respect to previously studied all-ferromagnetic crosses, as they also reduce the pinning potential at the junction and allow probing the magnetization locally. In addition, unusual metastable states with longitudinal domain walls along Py straight sections are observed. Micromagnetic simulations in combination with a magnetotransport model allow interpretation of the results and identification of the observed transitions.
Improved memory for information learnt before alcohol use in social drinkers tested in a naturalistic setting
Authors: Carlyle Molly , Dumay Nicolas , Roberts Karen , McAndrew Amy , Stevens Tobias , Lawn Will , Morgan Celia J. A. 
Published: July 2017

AbstractAlcohol is known to facilitate memory if given after learning information in the laboratory; we aimed to investigate whether this effect can be found when alcohol is consumed in a naturalistic setting. Eighty-eight social drinkers were randomly allocated to either an alcohol self-dosing or a sober condition. The study assessed both retrograde facilitation and alcohol induced memory impairment using two independent tasks. In the retrograde task, participants learnt information in their own homes, and then consumed alcohol ad libitum. Participants then undertook an anterograde memory task of alcohol impairment when intoxicated. Both memory tasks were completed again the following day. Mean amount of alcohol consumed was 82.59 grams over the evening. For the retrograde task, as predicted, both conditions exhibited similar performance on the memory task immediately following learning (before intoxication) yet performance was better when tested the morning after encoding in the alcohol condition only. The anterograde task did not reveal significant differences in memory performance post-drinking. Units of alcohol drunk were positively correlated with the amount of retrograde facilitation the following morning. These findings demonstrate the retrograde facilitation effect in a naturalistic setting, and found it to be related to the self-administered grams of alcohol.
Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles
Authors: Jung Anna Lena , Herkt Christina Elena , Schulz Christine , Bolte Kathrin , Seidel Kerstin , Scheller Nicoletta , Sittka-Stark Alexandra , Bertrams Wilhelm , Schmeck Bernd 
Published: July 2017

AbstractExtracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation. We observed an increase of exosomes in the 100 k pellet by nanoparticle tracking analysis, electron microscopy, and protein markers. This fraction additionally contained Legionella LPS, indicating also the presence of OMVs. In contrast, vesicles in the 16 k pellet, representing microparticles, decreased during infection. The 100 k vesicle fraction activated uninfected primary human alveolar epithelial cells, A549 cells, and THP-1 cells. Epithelial cell activation was reduced by exosome depletion (anti-CD63, or GW4869), or blocking of IL-1β in the supernatant. In contrast, the response of THP-1 cells to vesicles was reduced by a TLR2-neutralizing antibody, UV-inactivation of bacteria, or – partially – RNase-treatment of vesicles. Taken together, we found that during L. pneumophila infection, neighbouring epithelial cells were predominantly activated by exosomes and cytokines, whereas myeloid cells were activated by bacterial OMVs.
Projecting the effects of climate change on Calanus finmarchicus distribution within the U.S. Northeast Continental Shelf
Authors: Grieve Brian D. , Hare Jon A. , Saba Vincent S. 
Published: July 2017

AbstractCalanus finmarchicusis vital to pelagic ecosystems in the North Atlantic Ocean. Previous studies suggest the species is vulnerable to the effects of global warming, particularly on the Northeast U.S. Shelf, which is in the southern portion of its range. In this study, we evaluate an ensemble of six different downscaled climate models and a high-resolution global climate model, and create a generalized additive model (GAM) to examine how future changes in temperature and salinity could affect the distribution and density ofC.finmarchicus. By 2081–2100, we project averageC.finmarchicusdensity will decrease by as much as 50% under a high greenhouse gas emissions scenario. These decreases are particularly pronounced in the spring and summer in the Gulf of Maine and Georges Bank. When compared to a high-resolution global climate model, the ensemble showed a more uniform change throughout the Northeast U.S. Shelf, while the high-resolution model showed larger decreases in the Northeast Channel, Shelf Break, and Central Gulf of Maine.C.finmarchicusis an important link between primary production and higher trophic levels, and the decrease projected here could be detrimental to the North Atlantic Right Whale and a host of important fishery species.