bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics insights into the energy compensation of rumen microbiota of grazing yaks in cold season
Authors: Bai Jie , Tang Lijuan , Bi Yanliang , Li Mingliang 
Published: October 2024

BackgroundThe ability of yaks to adapt to the extreme environment of low temperatures and hypoxia at cold seasons on the Qinghai-Tibet Plateau (QTP) is related to the host genome; however, the convergent evolution of rumen microbiomes in host adaption is unknown.MethodsHere, we conducted a multi-omics study on the rumen fluid of grazing yaks from warm (July) and cold (December) seasons on the QTP to evaluate the convergent evolution of rumen microbiomes in the adaptation of grazing yaks to cold-seasons environments.ResultsThe results showed that grazing yaks at cold seasons had higher fibrolytic enzyme activities and volatile fatty acids (VFAs) concentrations, and the relative abundance of Firmicutes and the ratio Firmicutes to Bacteroidetes was significantly higher than that of yaks at warm seasons. Macrogenomic analyses showed that genes involved in forming VFAs and arginine were significantly enriched in cold-season yaks. Transcriptome analyses of the rumen epithelium showed that 72 genes associated with VFAs absorption and transport were significantly upregulated in cold-season yaks. Metabolomic analyses showed that the levels of ornithine, related to efficient nitrogen utilization, were significantly upregulated in cold-season yaks.ConclusionThe synergistic role of rumen microbiomes in the adaptation of grazing yaks to extreme environments at cold seasons was revealed by multi-omics study.
Efficacy of transcranial magnetic stimulation treatment in reducing neuropsychiatric symptomatology after traumatic brain injury
Authors: Riccitelli Gianna Carla , Borgonovo Riccardo , Villa Mariasole , Pravatà Emanuele , Kaelin-Lang Alain 
Published: October 2024

BackgroundNeuropsychiatric disorders are highly disabling in traumatic brain injury (TBI) patients, and psychopharmacological treatments often fail to adequately mitigate their detrimental effects. Repetitive transcranial magnetic stimulation (rTMS) is an emerging treatment in neurology and psychiatry, showing potential in treating psychiatric disorders.ObjectiveThis study investigates the efficacy of a novel, dual-site sequential rTMS protocol designed to treat neuropsychiatric symptoms in a TBI patient who was refractory to conventional treatments.MethodsA 34-year-old woman with severe head trauma and complex psychopathology underwent 20 daily sessions of focal-coil rTMS, combining inhibitory stimulation (1 Hz) on the right dorsolateral prefrontal cortex (DLPFC) and excitatory (10 Hz) on the left DLPFC, guided by a neuronavigation system. Psychiatric and neurocognitive assessments were conducted at baseline and at 2, 4, and 8 weeks following the beginning of rTMS treatment.ResultsAfter 2 weeks of treatment, the patient showed decreased impulsivity and obsessive-compulsive symptoms, along with improvements in attention and processing speed. After 4 weeks, impulsivity further declined, though no other significant changes were noted. At 8 weeks, a persistent positive effect was observed, including enhanced positive emotions.DiscussionThese findings suggest that guided, alternating neurostimulation of the DLPFC may modulate activity within cortico-striato-thalamo-cortical circuits, providing a promising alternative for managing neuropsychiatric symptoms in TBI patients who are resistant to traditional treatments.
Bibliometric analysis of the interplay between epilepsy and microglia: trends, hotspots, and emerging research areas
Authors: Zhang Shouye , Yang Xiaotong , Wang Yuping 
Published: October 2024

BackgroundEpilepsy, a common neurological disorder, has been increasingly associated with neuroinflammation, especially microglia activation. To gain insights into the research trends and patterns in this intersection, we conducted a bibliometric analysis of studies published between 2005 and 2024. Using the Web of Science Core Collection, we identified 1,229 relevant articles and reviews, focusing on the relationship between epilepsy and microglia.MethodsWe employed the Bibliometrix R package and VOSviewer to analyze the data. Our search strategy combined epilepsy-related terms with microglia and microglial cell keywords. The analysis encompassed publication trends, country and institutional contributions, journal sources, authors, keywords, and thematic evolution.ResultsThe number of publications has steadily increased, particularly after 2019, indicating growing research interest. The United States, China, and Germany emerged as the most productive countries, with extensive collaboration between China and the United States. Epilepsia and Journal of Neuroinflammation were the most influential journals. Aronica E, Vezzani A, and Engel T were the most prolific authors. Thematic analysis revealed clusters focused on temporal lobe epilepsy, epilepsy-related disorders, and microglia activation. Over the past several years, research has shifted from fundamental brain function studies to in-vivo investigations of specific molecular mechanisms. The CSTB (−) mouse model and NF-κB signaling pathway both merit further in-depth investigation.ConclusionIn conclusion, this bibliometric study reveals a surge in epilepsy-microglia research, led by key countries, journals, and researchers. Temporal lobe epilepsy, epilepsy-related disorders, and microglia activation are focal themes. Future directions include exploring microglia activation mechanisms, utilizing animal models, and interdisciplinary approaches.
Survival outcomes of patients with diffuse large B-cell lymphoma undergoing autologous stem cell transplantation in Germany: real-world evidence from an administrative database between 2010 and 2019
Authors: Heger Jan-Michel , Borchmann Peter , Riou Sybille , Werner Barbara , Papadimitrious Michael S. , Mahlich Jörg 
Published: October 2024

BackgroundLimited real-world evidence is available for patients with diffuse large B-cell lymphoma (DLBCL) who received an autologous stem cell transplantation (ASCT) in Germany.ObjectivesThis study aims to describe the real-world survival outcomes of patients with DLBCL who received ASCT in Germany after diagnosis.DesignThis study is a retrospective database analysis covering the period between 2010 and 2019.MethodsUnadjusted overall survival (OS) was plotted using the Kaplan–Meier estimator for the overall population and stratified by relapse status. A Cox regression was run to identify factors that influence OS.ResultsA total of 112 patients received an ASCT, with the average time from first-line treatment to ASCT being 11.7 months. The median OS estimated by Kaplan–Meier was 83.4 months for the entire cohort. The only variable that significantly reduced the OS was the presence of subsequent treatment after ASCT in a time-dependent model.ConclusionOS after ASCT for DLBCL patients in Germany is higher than previously reported and may still be considered a valid option for carefully selected patients with relapsed/refractory DLBCL.
Intercropping of tobacco and maize at seedling stage promotes crop growth through manipulating rhizosphere microenvironment
Authors: Ma Junmei , Liu Di , Zhao Peiyan , Dou Min , Yang Xiuhua , Liu Shulei , Nian Fuzhao , Tong Wenjie , Li Junying , Xu ZhaoLi , Zhang Liuchen , Zhang Hong , Li Yongzhong , Deng Xiaopeng , Liu Yating 
Published: October 2024

IntroductionChanges in the rhizosphere microbiome and metabolites resulting from crop intercropping can significantly enhance crop growth. While there has been an increasing number of studies on various crop combinations, research on the intercropping of tobacco and maize at seedling stage remains limited.MethodsThis study is the first to explore rhizosphere effects of intercropping between tobacco and maize seedling stages, we analyzed the nitrogen, phosphorus and potassium nutrients in the soil, and revealed the important effects on soil microbial community composition and metabolite profiles, thereby regulating crop growth and improving soil balance.Results and discussionCompared with mono-cropping, intercropping increased the biomass of the two crops and promoted the nutrient absorption of nitrogen, phosphorus and potassium. Under intercropping conditions, the activities of sucrase, catalase and nitrate reductase in tobacco rhizosphere soil and the content of available potassium, the activities of nitrate reductase and acid phosphatase in maize rhizosphere soil were significantly increasing. Rhizosphere soil bacterial and fungal communities such as Sphingomonas, Massilia, Humicola and Penicillium respond differently to crop planting patterns, and soil dominant microbial communities are regulated by environmental factors such as pH, Organic Matter, Available Potassium, Nitrate Reductase, and Urease Enzyme. Network analysis showed that soil microbial communities were more complex after intercropping, and the reciprocal relationship between bacteria and fungi was enhanced. The difference of metabolites in soil between intercropping and monocropping system was mainly concentrated in galactose metabolism, starch and sucrose metabolism pathway, and the content of carbohydrate metabolites was significantly higher than that of monocropping soil. Key metabolites such as D-Sucrose, D-Fructose-6-Phosphate, D-Glucose-1-Phosphatel significantly influence the composition of dominant microbial communities such as Sphingomonas and Penicillium. This study explained the effects of intercropping between flue-cured tobacco and maize on the content of soil metabolites and soil microbial composition in rhizosphere soil, and deepened the understanding that intercropping system can improve the growth of flue-cured crops seedlings through rhizosphere effects.
Inefficient and unique processing of social–emotional interference in school-aged children with high-functioning autism spectrum disorder
Authors: Chen Qing-Xin , Chen Qi , Zhai Kun , Chen Hui-Ting , Wu Yu-Lan , Liu Jin-Ming , Jin Yu 
Published: October 2024

IntroductionInterest is growing in investigating the ability of children with autism spectrum disorder (ASD) to process social information under conflicting and complex environments. However, few studies have employed objective behavioral measures to directly explore the underlying profile of social–emotional interference control.MethodsIn the current study, 53 children with ASD and 53 typically developing (TD) control, aged 6–12 years, completed a set of modified flanker tasks involving arrows, schematic faces, same real faces (with facial interference by the same person), and different real faces (with facial interference by different people), respectively. Response time in incongruent (RTInc) and congruent conditions (RTCon), percentage of errors in incongruent (%ErrorInc) and congruent conditions (%ErrorCon), and flanker effect calculated by ΔRT = (RTInc − RTCon)/RTCon and Δ%Error = %ErrorInc − %ErrorCon were used as outcome metrics.ResultsWe obtained three major results: (1) the ASD group had longer RTInc and RTCon compared to the TD group in the arrow, schematic-face, and same real-face tasks; (2) compared with the performance in the arrow flanker task, both groups exhibited longer RTs and reduced ΔRTs in the same real-face task; however, in the schematic-face task, longer RT and reduced ΔRT were exhibited in the TD group, but not in the ASD group; and (3) in the different real-face task, ASD group had higher %Error than the TD group, and %Error was negatively correlated with RT only in the ASD group.ConclusionThe current study delineates the inefficient processing of social–emotional interference in school-aged children with ASD and further suggests that these children might adopt a relatively optimized strategy like symbolization when dealing with emotional conflict. However, such compensatory cognitive strategies may be exhausted along with the increase in information load. These findings provide new perspectives of considering the difference more than difficulty in the cognitive profile of ASD, which will benefit the development of targeted behavioral interventions.
Family happiness and college students’ smartphone addiction control: the chain mediation effect of emotion regulation and self-control
Authors: Yin Xiangju , Yu Yongli , Qian Hongwei , Wang Zixu 
Published: October 2024

BackgroundMore and more college students use smartphones for a long time every day. The problem of smartphone addiction has become a hot topic of social concern. It not only affects the physical and mental health of college students but also affects the development of families and society. To investigate the effect of family happiness on college students’ smartphone addiction, we conducted a questionnaire survey and analysis.MethodsIn this study, 214 college students were investigated using the adolescent family happiness questionnaire, college students’ smartphone addiction scale, self-control scale, and emotion regulation scale. The data were analyzed by Amos and SPSS26.0 software.ResultsThe results showed that: (1) Family happiness, emotion regulation, and self-control were significantly inversely correlated with smartphone addiction; (2) Emotion regulation and self-control served as partial mediators in the linkage between family happiness and smartphone addiction, and the chain mediation effect of emotion regulation and self-control was significant.ConclusionFamily happiness, emotion regulation, and self-control are key factors that significantly influence college students’ smartphone addiction behavior. Family happiness not only has a direct effect on college students’ smartphone addiction behavior but also has an indirect effect on it through the chain mediation effect of emotion regulation and self-control.
NBF2, an Algal Fiber-Rich Formula, Reverses Diabetic Dyslipidemia and Hyperglycemia In Vivo
Authors: Ngatu Nlandu Roger , Hossain Akram , Maruo Nao , Akumwami Steeve , Rahman Asadur Md. , Eitoku Masamitsu , Kanda Kanae , Nishiyama Akira , Suganuma Narufumi , Hirao Tomohiro 
Published: October 2024

Ulva prolifera, known as Aonori in Japan, is an edible alga species that is mass-cultivated in Japan. Supplementation with Aonori-derived biomaterials has been reported to enhance metabolic health in previous studies. This was an experimental study that evaluated the metabolic health effects of NBF2, a formula made of algal and junos Tanaka citrus-derived biomaterials, on obesity and type 2 diabetes (T2DM). We used 18 obese and hyperglycemic Otsuka Long-Evans Tokushima Fatty (OLETF) rats that were assigned randomly to three groups of six animals: a high-dose NBF2 drink (20 mg/kg) group, a low-dose (10 mg/kg) NBF2 drink group and the control group that received 2 mL of tap water daily for a total of six weeks. We also used eight LETO rats as the normal control group. In addition to the glucose tolerance test (OGTT), ELISA and real-time PCR assays were performed. High-dose and lowdose NBF2 improved insulin sensitivity, as well as glycemic and lipid profiles, as compared with control rats. The OGTT showed that both NBF2 groups and LETO rats had normalized glycemia by the 90-min time-point. NBF2 up-regulated PPARα/γ-mRNA and Sirt2-mRNA gene expressions in BAT and improved the blood pressure profile. These findings suggest that the NBF2 formula, which activates PPAR-α/γ mRNA and Sirt2-mRNA, may reverse dyslipidemia and hyperglycemia in T2DM.
A pH-Sensitive I-Motif Aptamer Probe for Dual Targeting and Imaging of Pancreatic Cancer Cells via Nucleolin Interactions
Authors: Li Quwen , Chen Qingquan , Tang Juan , Liu Qicai , Gao Feng 
Published: October 2024

This study investigates the targeted imaging and tumor inhibition effects of a “signal-off” imaging-based activatable aptamer probe (I-AAP) for pancreatic cancer. AS1411, contained in the I-AAP probe, targets the nucleolin and i-motifs on the membrane surfaces of pancreatic cancer cells (PANC-1) and has a dual response to pH and an acidic environment. In vitro, a “signal-off” type I-AAP probe was constructed that possesses a fluorescence effect in a phosphate buffer solution at pH 6.0 and 6.5. The probe has no fluorescence effect at pH 7.0 or 7.5; thus, the “signal-off” type I-AAP probe has a targeted acid-sensitive response in pancreatic cancer and a tumor microenvironment imaging effect. After incubation for 30 min, AS1411 was taken up by PANC-1 cells and reached its peak value within 2 h. AS1411 inhibited pancreatic cancer cells in a time-dependent manner. This “signal-off” I-AAP probe is expected to be suitable for a low-background and high-specificity molecular pathologic tracer technique, making use of the presence of nucleolins on the surface of pancreatic cancer cells and the low pH response of the tumor microenvironment.
When Games get in the way: Gamification Approach for Teaching Maths – A Case Study With Undergraduate Tourism Students
Authors: Pais Sónia , Hall Andreia 
Published: October 2024

Planning more dynamic and motivating lessons for students is the goal of any teacher, especially maths teachers, a subject where the lack of interest and motivation to learn is so evident and a major concern. Several studies highlight the positive impact of the adoption of gamification strategies in teaching/learning, both in student motivation and in the development of soft skills (teamwork, creativity, decision-making, leadership, communication, and critical thinking). Game-based learning thus promotes students' interest and involves them in this process. In this sense, one of the authors of this study chose to use several (non-digital) games to support the teaching-learning process in her classes. The games used were developed by the authors and have been thoroughly designed to address specific maths topics. The games took various forms, including card games, board games and other hands-on activities. The participants in the study are first-year undergraduate students from a Portuguese higher education institution and encompassed the course of “Quantitative Methods”. The aim of the study is to investigate students’ perceptions of the use of gamification in the maths teaching-learning process. To gather information about students’ insights on the use of games in math classes a quantitative survey was conducted. Similar studies have shown that higher education students are usually receptive to the use of games, finding it useful to increase their motivation and consider game-based learning can positively impact learning. Our findings are in line with those studies.
Escaping Online: Teacher Student Engagement in a Digital Escape Game
Authors: Cruaud Caroline 
Published: October 2024

Escape games have become popular as a learning activity and are being used in a large variety of subjects (e.g., chemistry (Peleg et al, 2019), music (Babazadeh et al, 2022)). Research has shown that they are generally engaging for students (Veldkamp et al, 2020). However, the study of engagement is often limited to its observable aspect (e.g., the students are having fun; they are participating in the learning activity) or is not the focus of the analysis. Fewer studies are also investigating the use of escape games in fully online settings, when students are each playing from their own location.
This research study is exploring engagement from the students’ perspective, within a fully online course in a teacher education program at a Norwegian university. Teacher students were invited to play Radioaktiv, a digital escape game for natural sciences on the topic of radioactivity. Six pairs of students also participated in guided interviews after playing. Reflexive thematic analysis of the interview data with the students was conducted to investigate what characterised the students’ experience of engagement while playing the online escape game Radioaktiv. Through this research question, the study is taking a closer look at what it means when the students say they were engaged in the game. Findings show four main aspects of the students’ experience of engagement. First, the time pressure was found to be a double-edged sword. Students found the timer engaging, but some were also too stressed by its presence to the detriment of learning outcomes. Second, students were engaged in completing tasks for their own sake, independently from other game aspects. Third, students appreciated having a read thread, but were not engaged in the specific narrative of the escape game. Finally, students positioned themselves as teachers and it strongly defined their experience of engagement in the game. They saw the game through the potential it held for their own pupils. These findings raise questions about the selection of escape games for learning in online environments and when and how to use them (e.g., discover a new theme, repetition, focus on soft skills).
التقنيات التصميمية والسمات الجمالية في تنفيذ الاعمال الفنية لدى طلبة قسم التربية الاسرية والمهن الفنية
Authors: ا.د.حسين محمد علي ساقي  
Published: October 2024

يهد ف البحث الحالي الى الكشف عن انواع التقنيات التصميمية والسمات الجمالية في تنفيذ الاعمال الفنية وتم تحديد اعمال طلبة قسم التربية الاسرية والمهن الفنية للعام الدراسي 2023ـ224 ثم تحديد المصطلحات الواردة في عنوان البحث .وفي الفصل الثاني مبحثين المبحث الاول عن التقنيات التصميمية ثم السمات الجمالية . ومؤشرات الاطار النظري . وفي الفصل الثالث منهجية البحث اذ اتبع المنهج الوصف والتحليل وتحديد المجتمع . حيث اختار الباحث ( 3) عملا فنيا من المجتمع الاصلي قصديا كونها تتلائم مع متطلبات البحث الحالي وبنسبة 5%, وقام الباحث ببناء اداة التحليل وتم التحقق من الصدق والثبات بعرضه على مجموعة من الخبراء وبعد الانتهاء من التحليل اظهرت النتائج التي توصل اليه الباحث الى ان التقنيات التصميمية تظهر فاعليتها في الاعمال الفنية بصورة متقنة الا ان النتاجات تتحقق من خلال الية التقنيات المعاصرة.و القيمة الجمالية لاتحقق في العمل الفني الا بفعل التقنيات المتنوعة كالرسم والخط والاشكال والرموز .وهذا النظرة نجدها في نجاح خصوصية العمل الفني على المستوى الجمالي, وان الجمال لايتحقق كليا ما لم تحقق هذه العناصر.ثم الاستنتاجات والتوصيات واهم المقترحات القيام بالدراسات التالية :التقنيات الحديثة والية انتاجها التفاعلي في اللوحات الخطية .وتنوع التقنية والخامة في الفنون التشكيلية المعاصرة. ثم اهم المصادر المستخدمة في البحث .و استمارة التحليل
الذكاء الاستنتاجي لدى طلبة كلية التربية الاساسية
Authors: أ.د.محمد عبد الكريم طاهر  , أ.م.د.حيدر شمسي حسن  , أ.د.رحيم علي صالح  
Published: October 2024

يعد موضوع الذكاء من المواضيع الحيوية التي اهتم بها العلماء نظرا لارتباطه بميادين الحياة المختلفة الاكاديمية والمهنية والفنية والاجتماعية وغيرها، اذ ان الدماغ الانساني الذي يمثل الجزء الاساسي في الجهاز العصبي المركزي هو في غاية التعقيد من حيث التركيب، ويعمل بغاية الدقة والكفاءة، وما التقدم الذي حققته الانسانية عبر التاريخ بفضل القدرة الذهنية العالية التي يتمتع بها الدماغ، والجانب المهم من وظائف تلك القدرة هو الذكاء. تهدف الدراسة الى تعرف مستوى.الذكاء الاستنتاجي لدى.طلبة كلية التربية الاساسية لقسمي الرياضيات والارشاد النفسي والتربوي، وقام الباحثون ببناء اختبار الذكاء الاستنتاجي والذي تكون بصيغته النهائية من (44) فقرة وبأربعة بدائل، بعد ان تم أستخراج صدق وتمييز الفقرات ، وتم استخراج صدق وثبات الاختبار بأستخدام الوسائل الاحصائية المناسبة وبمساعدة البرنامج الاحصائي spss،وتم تطبيق الاختبار.على عينة التحليل الاحصائي البالغة (400) طالب وطالبة ، وتم تطبيق الاختبار.على عينة التطبيق. النهائي البالغة (200) طالب.وطالبة وكانت النتائج: ان اختبار الذكاء الاستنتاجي يتمتع بخصائص قياسية جيدة ،ووجود درجة عالية من الذكاء الاستنتاجي لدى عينة البحث الكلية , ووجود فروق دالة احصائيا بين عينة الذكور وعينة الاناث في الذكاء الاستنتاجي ولصالح عينة الذكور, ووجود فروق دالة احصائيا بين عينة التخصصين العلمي والانساني ولصالح عينة التخصص العلمي , وقد أوصت الدراسة امكانية استخدام الاختبار من قبل رؤساء الاقسام في تشخيص الطلبة الذين لديهم ذكاء استنتاجي عالي ، وخرجت الدراسة بعدد من.الاستنتاجات والمقترحات.