bluuebunny/crossref_metadata_2025_split huggingface dataset:
Изоферментный профиль аденозиндезаминазы в аденокарциноме молочной железы
Authors: Барсукова М. А. , Хомутов Е. В. , Дмитриев Л. С. , Якубенко Е. Д. , Зинкович И. И. 
Published: November 2017

Резюме: Изменение активности АДА сопряжено со многими патологическими процессами, в том числе с малигнизацией. В организме человека функционируют и проявляют плейотропные свойства две изоформы АДА: АДА1 и АДА2.
Целью работы является исследование особенностей распределения активности изоферментов АДА в аденокарциноме молочной железы и смежной с ней тканью.
Материалы и методы. Материалом послужили биоптаты аденокарциномы молочной железы и смежной с ней ткани (TxNxMx). Исследуемые изоферменты выделяли методом гель-фильтрации, ферментативную активность определяли спектрофотометрически.
Результаты. Обнаруженная в узлах аденокарциномы высокая активность АДА обусловлена гиперэкспрессией обоих ее изоферментов. Так, активности АДА1 и АДА2 в опухолевых узлах в среднем превышают таковые в смежной ткани в 2,1 и 2,2 раза, соответственно. Установлено, что в обеих группах изученных образцов (опухолевый узел и смежная с ним ткань) преобладает экспрессия АДА1. В биоптатах смежной ткани в среднем ее активность в 5,5 раз превышает соответствующий показатель для АДА2. В узлах опухолевой ткани активность АДА1 в среднем выше активности АДА2 в 4,9 раза.
Заключение. Показанная нами высокая активность обоих изоферментов АДА является характерной для озлокачествленной железистой ткани. Показатель соотношения активностей изоферментов АДА1 и АДА2 сохраняется на одном уровне как в опухолевом узле, так в смежных с ним тканях, не имеющих признаков злокачественной трансформации.
Влияние старения на окислительный потенциал плазмы крови у онкологических больных
Authors: Зуйков С. А. , Зинкович И. И. 
Published: November 2017

Цель: изучить состояния про- и антиоксидантной системы, а также показатели свободно-радикального окисления белков плазмы крови у больных раком желудка в зависимости от возраста.
Материалы и методы: материалом исследования послужила плазма крови 35 больных раком желудка в возрасте 40-79 лет. Изучили активность ферментов обмена пуриновых нуклеотидов – аденозиндезаминазу (АДА) и ксантиноксидазу (КО), как показатели прооксидантной системы; антиоксидантную защиту – глутатионпероксидазу (ГПО) и супероксиддисмутазу (СОД); показатели окислительной модификации белков (ОМБ) – 2,4-динитрофенилгидразоны. Методом изучения активностей ферментов и уровня 2,4‑динитрофенилгидразонов была спектрофотометрия.
Результаты: установлено, что при старении организма у больных происходит усиление прооксидантного статуса плазмы крови, за счет повышения активностей аденозиндезаминазы и ксантиноксидазы, а также уровня 2,4‑динитрофенилгидразонов на фоне снижения активностей ферментов антиоксидантной защиты – супероксиддисмутазы и глутатионпероксидазы.
Выводы: обнаружены разнонаправленные изменения активностей ферментативного звена антиоксидантной защиты у больных раком желудка.
PENGARUH PENDEKATAN PEMBELAJARAN KETERAMPILAN PROSES DENGAN MODEL PEMBELAJARAN PROBLEM BASED INSTRUCTION TERHADAP PENINGKATAN KEMAMPUAN BERPIKIR KRITIS MAHASISWA PADA MATA KULIAH PENDIDIKAN KEPENDUDUKAN DAN LINGKUNGAN HIDUP (PKLH)
Authors: Astuti Irnin Agustina Dwi , Ahmad Deni Nasir 
Published: November 2017

Telah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pendekatan pembelajaran keterampilan proses dengan model pembelajaran Problem Based Instruction (PBI) terhadap peningkatan kemampuan berpikir kritis. Pendidikan Kependudukan dan Lingkungan Hidup (PKLH) merupakan sebuah proses yang bertujuan dalam membangun populasi dunia yang berkesadaran dan memiliki kepedulian terhadap lingkungan secara keseluruhan dari berbagai problem yang terkait dengannya dan merupakan salah satu mata kuliah di Universitas Indraprasta PGRI. Secara umum mahasiswa hanya memahami secara konsep saja mengenai lingkungan hidup tetapi masih kurang dalam konkret atau nyata. Subyek penelitian ini adalah mahasiswa Pendidikan Fisika semester 6 dengan sampel peserta didik kelas R6A sebagai kelas eksperimen dan kelas R6B sebagai kelas kontrol. Metode penelitian yang digunakan adalah metode quasi eksperimen dengan teknik analisis menggunakan uji t. Kelas eksperimen menggunakan model pembelajaran PBI, sedangkan kelas kontrol menggunakan pembelajaran studi kasus. 1. Terjadi peningkatan kemampuan berpikir kritis mahasiswa dengan Pendekatan Pembelajaran Keterampilan Proses dengan metode atau model Problem Based Instruction (PBI) pada mata kuliah Pendidikan Kependudukan dan Lingkungan Hidup (PKLH); 2) Terjadi peningkatan Kemampuan Berpikir Kritis dengan metode Pemberian Tugas Studi Kasus pada mata kuliah Pendidikan Kependudukan dan Lingkungan Hidup (PKLH); dan 3) Terjadi perbedaan hasil pembelajaran dimana metode atau model pembelajaran Problem Based Instruction lebih tinggi atau lebih baik dari pada metode pembelajaran Pemberian Tugas Studi Kasus pada mata kuliah Pendidikan Kependudukan dan LingkunganHidup (PKLH).
PENGARUH DOSIS PUPUK NPK MAJEMUK DAN PGPR (PLANT GROWTH PROMOTING RHIZOBACTERIA) TERHADAP BOBOT BASAH DAN KADAR KLOROFIL DAUN TANAMAN PAKCOY (Brassica rapa L.)
Authors: Rosyida Rosyida , Nugroho Ary Susatyo 
Published: November 2017

Plant Growth Promoting Rhizobacteria (PGPR) ialah kelompok mikroorganisme tanah yang menguntungkan bagi kesuburan tanah dan tanaman. Mikroorganisme yang hidup di daerah perakaran tersebut memiliki 3 peran utama bagi tanaman yaitu : 1) sebagai biofertilizer, 2) sebagai biostimulan, dan 3) sebagai bioprotektan. Penelitian ini bertujuan untuk memperlihatkan efek suplementasi PGPR dalam dosis pemberian pupuk majemuk NPK terhadap bobot basah dan kadar klorofil daun pada tanaman pakchoy (Brassica rapa L.). Perlakuan pada penelitian ini meliputi 5 taraf perlakuan, yaitu: variasi kombinasi dosis pupuk majemuk NPK dan PGPR: NPK 100% (A0P0), NPK 75% + PGPR 25% (A1P1), NPK 50% + PGPR 50% (A2P2), NPK 25% + PGPR 75% (A3P3), PGPR 100% (A4P4). Masing – masing perlakuan diulang 5 kali. Desain percobaan adalah Rancangan Acak Lengkap (RAL), hasil percobaan dianalisis dengan ANOVA (P < 0,05), dilanjutkan dengan uji Duncan. Berdasarkan hasil percobaan, disimpulkan bahwa bahwa ada pengaruh yang nyata pada suplementasi PGPR dalam dosis pupuk majemuk NPK pada parameter bobot basah dan kadar klorofil daun tanaman pakchoy. Bobot basah tertinggi ditunjukkan pada tanaman pakchoy yang diberi perlakuan NPK 25% + PGPR 75% (A3P3), sedangkan pada tanaman yang diberi perlakuan NPK 75% + PGPR 25% (A1P1) mampu mengakumulasi kadar klorofil tertinggi diantara daun tanaman lainnya. Kata Kunci: Plant Growth Promoting Rhizobacteria (PGPR), Pupuk, NPK, Pakchoy
Naming, Claiming, and Framing Marie Chauvet
Authors: Jean-Charles Régine Michelle 
Published: November 2017

Abstract
This essay offers a metacritical reading of the discourses surrounding Haitian author Marie Chauvet (1916–1973). I argue that paying attention to the naming (how she is described), claiming (how scholars and authors define their investment in her work), and framing (how her work is analyzed by scholars) of Chauvet reveals a dominant discourse that influences our study of the author. Taking a feminist approach to the interpretations of her work, I argue that ultimately these Chauvet narratives reveal more about anxieties surrounding gender than they do about her body of work.
Different patterns of dopaminergic and serotonergic dysfunction in manic, depressive and euthymic phases of bipolar disorder
Authors: Müller Hans-Wilhelm , Hautzel Hubertus , Nikolaus Susanne 
Published: November 2017

SummaryA variety of alterations in brain neurotransmitter systems has been proposed as the cause of bipolar disorder (BD). We conducted a PUBMED search, which provided a total of 45 in vivo investigations with PET and SPECT, in which binding to serotonin transporter (SERT), 5-HT1A receptor (R), 5-HT2AR, dopamine transporter (DAT), vesicular monoamine transporter (VMAT2), D1R, D2R, muscarinic M2R and nicotinic ß2-nAChR as well as dopamine synthesis and/or dopamine release were assessed in BD patients in the manic (6 studies, 39 patients, 77 controls), depressive (15 studies, 248 patients, 488 controls) or eu- thymic condition (18 studies, 265 patients, 293 controls) and in mixed collectives of BD patients (6 studies, 55 patients, 80 controls). The retrospective analysis revealed a complex pattern of dysregulations within and between neurotransmitter systems, which is causally linked to the acute and euthymic states of BD. While increased mesencephalic, limbic and parietotemporoccipital serotonin and increased frontal dopamine underlie mania, the depressive state is characterized by decreased frontal and limbic serotonin, increased frontal and limbic acetylcholine and increased frontal dopamine. Also in euthymia, no normalization of receptor and transporter densities was observed. Alterations of regulation states of bindings sites, however, act together to achieve a normalization of mesencephalic, limbic and cortical serotonin.
Quand les morts passent un scanner
Authors: Schnegg Céline , Rey Séverine 
Published: November 2017

Lors de son passage en médecine légale, dans les cas d’homicides ou de morts dites suspectes, le cadavre subit une série d’épreuves techniques et scientifiques, qui visent à le “faire parler” et à identifier les causes du décès. En plus de l’autopsie conventionnelle, des techniques d’imagerie médicale sont désormais intégrées dans le dispositif d’enquête médico-légale. Sur la base d’observations ethnographiques menées dans un centre suisse de médecine légale à la pointe de l’imagerie médico-légale, nous analysons les variations ontologiques du corps mort en lien avec sa prise en charge et les différentes techniques de visualisation utilisées par les experts. Les différentes « épreuves du corps », et tout particulièrement les techniques d’autopsie virtuelle, font varier les frontières entre vivant et mort, animé et inerte, personne et cadavre. La reconfiguration de ces frontières répond à des exigences scientifiques, techniques, mais aussi normatives et morales.
Éxodo a la “tierra prometida”: Del demonio y otros monstruos en la obra de Juan de Dios Mora
Authors: Miramontes Olivas Adriana , De Dios Mora Juan , Caplow Deborah 
Published: November 2017

Juan de Dios Mora es grabador y docente en la Universidad de Texas en San Antonio, donde comenzó a enseñar pintura, dibujo y grabado en 2010. Mora es un artista prolífico cuyos grabados han sido publicados en numerosos sitios incluyendo los catálogos New Arte Nuevo: San Antonio 2010 y New Art/Arte Nuevo San Antonio 2012. Este año su obra se exhibió en distintos lugares incluyendo el Museo de Arte McNay en San Antonio, Texas en Juan Mora: Cultural Clash (Juan Mora: Choque Cultural, junio 8 a agosto 13 de 2017) y en el Centro de Arte the Cole en la Galería Reavley en Nacogdoches, Texas, en Juan de Dios Mora. En 2016, Mora participó en la exhibición colectiva Los de abajo: Garbage as an Artistic Source (Los de abajo: la basura como una fuente artística) en el Centro Cultural Guadalupe en San Antonio (de junio 10 a julio 29 de 2016). Mora también ha sido curador de la exhibición Print It Up por varios años, organizada por el artista en el área del centro de San Antonio dándoles una visibilidad sin precedentes a numerosos artistas. Para esta exhibición Mora guía y aconseja tanto a estudiantes como a exalumnos sobre el proceso de exhibición, desde cómo crear un portafolio, enmarcar e instalar obras, hasta realizar contratos con galeristas y vender las obras al público. Adriana Miramontes Olivas es estudiante de doctorado en el Departamento de Historia del Arte y Arquitectura en la Universidad de Pittsburgh. Completó su licenciatura en la Universidad de Texas de El Paso y la maestría en la Universidad de Texas de San Antonio. Sus intereses en materia de investigación incluyen arte moderno y contemporáneo global con un enfoque en Latinoamérica, estudios de género, sexualidad e identidad nacional. La Dra. Deborah Caplow es historiadora del arte, curadora y autora del libro sobre el ilustrador Mexicano Leopoldo Méndez (Leopoldo Méndez: arte revolucionario y el grabado mexicano, publicado por la Universidad de Texas). Ella enseña historia del arte en la Universidad de Washington, Bothell. Sus áreas de investigación incluyen arte Mexicano del siglo XX, las intersecciones entre arte y política, y la historia de la fotografía. Actualmente hace investigación de grabados contemporáneos en Oaxaca, México.
CDK4 expression in chordoma: A potential therapeutic target
Authors: Liu Tang , Shen Jacson K. , Choy Edwin , Zhang Yu , Mankin Henry J. , Hornicek Francis J. , Duan Zhenfeng 
Published: November 2017

ABSTRACTChordomas are rare bone tumors and treatment is commonly based on a combination of surgery and radiotherapy. There is no standard chemotherapy treatment for chordoma. The aim of this study was to determine the expression of cyclin‐dependent kinase 4 (CDK4) in chordoma and its therapeutic implications. We evaluated CDK4 expression both in chordoma cell lines and in chordoma tissues. Also, we investigated the functional roles of CDK4 in chordoma cell growth and proliferation. Furthermore, the therapeutic implications of targeting CDK4 in chordoma were evaluated. We found CDK4 highly expressed in chordoma cell lines and in a majority (97.7%) of chordoma tissues. Higher CDK4 expression correlated with metastasis and recurrence of chordoma. Treatment of chordoma cells using CDK4 inhibitor palbociclib could efficiently inhibit chordoma cells growth and proliferation. These data demonstrate that targeting CDK4 may be useful as a novel strategy in the treatment of chordoma. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1581–1589, 2018.
Structural characterization of site-modified nanocapsid with monodispersed gold clusters
Authors: Stark Marie C , Baikoghli Mo A , Lahtinen Tanja , Malola Sami , Xing Li , Nguyen Michelle , Nguyen Marina , Sikaroudi Aria , Marjomäki Varpu , Häkkinen Hannu , Cheng R Holland 
Published: November 2017

AbstractHepatitis E Virus-like particles self-assemble in to noninfectious nanocapsids that are resistant to proteolytic/acidic mucosal delivery conditions. Previously, the nanocapsid was engineered to specifically bind and enter breast cancer cells, where successful tumor targeting was demonstrated in animal models. In the present study, the nanocapsid surface was modified with a solvent-exposed cysteine to conjugate monolayer protected gold nanoclusters (AuNC). Unlike commercially available gold nanoparticles, AuNCs monodisperse in water and are composed of a discrete number of gold atoms, forming a crystalline gold core. Au102pMBA44 (Au102) was an ideal conjugate given its small 2.5 nm size and detectability in cryoEM. Au102 was bound directly to nanocapsid surface cysteines via direct ligand exchange. In addition, Au102 was functionalized with a maleimide linker (Au102_C6MI) for maleimide-thiol conjugation to nanocapsid cysteines. The AuNC-bound nanocapsid constructs were conjugated in various conditions. We found Au102_C6MI to bind nanocapsid more efficiently, while Au102 remained more soluble over time. Nanocapsids conjugated to Au102_C6MI were imaged in cryoEM for single particle reconstruction to localize AuNC position on the nanocapsid surface. We resolved five unique high intensity volumes that formed a ring-shaped density at the 5-fold symmetry center. This finding was further supported by independent rigid modeling.
Modulation Of Microtubule Acetylation By The Interplay Of TPPP/p25, SIRT2 And New Anticancer Agents With Anti-SIRT2 Potency
Authors: Szabó Adél , Oláh Judit , Szunyogh Sándor , Lehotzky Attila , Szénási Tibor , Csaplár Marianna , Schiedel Matthias , Lőw Péter , Jung Manfred , Ovádi Judit 
Published: November 2017

AbstractThe microtubule network exerts multifarious functions controlled by its decoration with various proteins and post-translational modifications. The disordered microtubule associated Tubulin Polymerization Promoting Protein (TPPP/p25) and the NAD+-dependent tubulin deacetylase sirtuin-2 (SIRT2) play key roles in oligodendrocyte differentiation by acting as dominant factors in the organization of myelin proteome. Herein, we show that SIRT2 impedes the TPPP/p25-promoted microtubule assembly independently of NAD+; however, the TPPP/p25-assembled tubulin ultrastructures were resistant against SIRT2 activity. TPPP/p25 counteracts the SIRT2-derived tubulin deacetylation producing enhanced microtubule acetylation. The inhibition of the SIRT2 deacetylase activity by TPPP/p25 is evolved by the assembly of these tubulin binding proteins into a ternary complex, the concentration-dependent formation of which was quantified by experimental-based mathematical modelling. Co-localization of the SIRT2-TPPP/p25 complex on the microtubule network was visualized in HeLa cells by immunofluorescence microscopy using Bimolecular Fluorescence Complementation. We also revealed that a new potent SIRT2 inhibitor (MZ242) and its proteolysis targeting chimera (SH1) acting together with TPPP/p25 provoke microtubule hyperacetylation, which is coupled with process elongation only in the case of the degrader SH1. Both the structural and the functional effects manifesting themselves by this deacetylase proteome could lead to the fine-tuning of the regulation of microtubule dynamics and stability.
Management of Reducible Ventral Hernias
Authors: Wolf Lindsey L. , Ejiofor Julius I. , Wang Ye , Hunink Myriam G. , Losina Elena , Haider Adil H. , Smink Douglas S. 
Published: November 2017

Objective:
To compare long-term clinical and economic outcomes associated with 3 management strategies for reducible ventral hernia: repair at diagnosis (open or laparoscopic) and watchful waiting.

Background:
There is variability in ventral hernia management. Recent data suggest watchful waiting is safe; however, long-term clinical and economic outcomes for different management strategies remain unknown.

Methods:
We built a state-transition microsimulation model to forecast outcomes for individuals with reducible ventral hernia, simulating a cohort of 1 million individuals for each strategy. We derived cohort characteristics (mean age 58 years, 63% female), hospital costs, and perioperative mortality from the Nationwide Inpatient Sample (2003–2011), and additional probabilities, costs, and utilities from the literature. Outcomes included prevalence of any repair, emergent repair, and recurrence; lifetime costs; quality-adjusted life years (QALYs); and incremental cost-effectiveness ratios. We performed stochastic and probabilistic sensitivity analyses to identify parameter thresholds that affect optimal management, using a willingness-to-pay threshold of $50,000/QALY.

Results:
With watchful waiting, 39% ultimately required repair (14% emergent) and 24% recurred. Seventy per cent recurred with repair at diagnosis. Laparoscopic repair at diagnosis was cost-effective compared with open repair at diagnosis (incremental cost-effectiveness ratio $27,700/QALY). The choice of operative strategy (open vs laparoscopic) was sensitive to cost and postoperative quality of life. When perioperative mortality exceeded 5.2% or yearly recurrence exceeded 19.2%, watchful waiting became preferred.

Conclusions:
Ventral hernia repair at diagnosis is very cost-effective. The choice between open and laparoscopic repair depends on surgical costs and postoperative quality of life. In patients with high risk of perioperative mortality or recurrence, watchful waiting is preferred.
The asexual genome of Drosophila
Authors: Schiffels Stephan , Mustonen Ville , Lässig Michael 
Published: November 2017

AbstractThe rate of recombination affects the mode of molecular evolution. In high-recombining sequence, the targets of selection are individual genetic loci; under low recombination, selection collectively acts on large, genetically linked genomic segments. Selection under linkage can induce clonal interference, a specific mode of evolution by competition of genetic clades within a population. This mode is well known in asexually evolving microbes, but has not been traced systematically in an obligate sexual organism. Here we show that theDrosophilagenome is partitioned into two modes of evolution: alocal interferenceregime with limited effects of genetic linkage, and aninterference condensatewith clonal competition. We map these modes by differences in mutation frequency spectra, and we show that the transition between them occurs at a threshold recombination rate that is predictable from genomic summary statistics. We find the interference condensate in segments of low-recombining sequence that are located primarily in chromosomal regions flanking the centromeres and cover about 20% of theDrosophilagenome. Condensate regions have characteristics of asexual evolution that impact gene function: the efficacy of selection and the speed of evolution are lower and the genetic load is higher than in regions of local interference. Our results suggest that multicellular eukaryotes can harbor heterogeneous modes and tempi of evolution within one genome. We argue that this variation generates selection on genome architecture.Author SummaryTheDrosophilagenome is an ideal system to study how the rate of recombination affects molecular evolution. It harbors a wide range of local recombination rates, and its high-recombining parts show broad signatures of adaptive evolution. The low-recombining parts, however, have remained dark genomic matter that has been omitted from most studies on the inference of selection. Here we show that these genomic regions evolve in a different way, which involves clonal competition and is akin to the evolution of asexual systems. This regime shows a lower efficacy of selection, a lower speed of evolution, and a higher genetic load than high-recombining regions. We argue these evolutionary differences have functional consequences: protein stability and protein expression are gene traits likely to be partially compromised by low recombination rates.