bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do people with bipolar disorders have access to psychosocial treatments? A survey in Italy
Authors: Barbato Angelo , Vallarino Martine , Rapisarda Filippo , Lora Antonio , Parabiaghi Alberto , D’Avanzo Barbara , Lesage Alain 
Published: February 2016

Background: Several guidelines consider psychosocial treatments an essential component of clinical management of bipolar disorders in addition to drug therapy. However, to what extent such interventions are available in everyday practice to the average patient attending mental health services is not known. Aims: This study aims to investigate access of people with bipolar disorders to psychosocial treatments in a community-based care system. Method: Information on care delivery and service utilization were retrieved from the psychiatric database of Lombardy, Italy, covering a population of 9,743,000, for all adults who had at least one contact in 2009 with psychiatric services. Rates of patients with a diagnosis of bipolar disorder who had access to individual psychotherapy, couple/family therapy, group psychotherapy and family interventions were calculated and compared to patients with schizophrenia and depression. Results: A total of 8,899 subjects with bipolar disorder had been in contact with psychiatric services, corresponding to a treated annual prevalence rate of 1.1‰. More than 80% of patients were treated in community settings. Rates of patients receiving structured psychosocial treatments ranged from 0.7% for couple/family therapy to 6.1% for individual psychotherapy. No differences with patients with schizophrenia and depression were found. Patients with schizophrenia received more interventions labeled as rehabilitation. Conclusion: Few people with bipolar disorders had access to psychosocial treatments. Even in a well-developed system of community care, offer of psychosocial interventions for bipolar disorders is inadequate. This issue should be a target for future research on dissemination and implementation strategies.
Natural history and factors associated with ulnar-sided wrist pain in distal radial fractures treated by plate fixation
Authors: Kim J. K. , Kim D. J. , Yun Y. 
Published: February 2016

We documented longitudinal changes in the incidence of ulnar-sided wrist pain after distal radial fractures treated by plate fixation and identified factors associated with ulnar-sided wrist pain. A total of 140 patients were enrolled in this study. Radiographs were taken 3 months after operation, and were used to measure radial inclination, anterior angulation and ulnar variance, and to identify the presence of an ulnar styloid fracture. Clinical assessments at the same time included grip strengths, ranges of wrist motion and the patient-rated wrist evaluation questionnaire. The presence of ulnar-sided wrist pain was noted at each follow-up visit. The incidence of ulnar-sided wrist pain decreased significantly with time after surgery (22 patients at 3 months, 11 patients at 6 months and three patients at 12 months). The mean age, sex, the presence of an ulnar styloid fracture and the classification of the distal radial fracture were not factors that were associated with a higher incidence of ulnar-sided wrist pain, but there was an association between higher patient-rated wrist evaluation scores and the presence of ulnar-sided wrist pain. Level of evidence: Prognosis, Level IV
Test-Retest Reliability of Isokinetic Knee Strength Measurements in Children Aged 8 to 10 Years
Authors: Fagher Kristina , Fritzson Annelie , Drake Anna Maria 
Published: February 2016

Background: Isokinetic dynamometry is a useful tool to objectively assess muscle strength of children and adults in athletic and rehabilitative settings. This study examined test-retest reliability of isokinetic knee strength measurements in children aged 8 to 10 years and defined limits for the minimum difference (MD) in strength that indicates a clinically important change. Hypothesis: Isokinetic knee strength measurements (using the Biodex System 4) in children will provide reliable results. Study Design: Descriptive laboratory study. Methods: In 22 healthy children, 5 maximal concentric (CON) knee extensor (KE) and knee flexor (KF) contractions at 2 angular velocities (60 deg/s and 180 deg/s) and 5 maximal eccentric (ECC) KE/KF contractions at 60 deg/s were assessed 7 days apart. The intraclass correlation coefficient (ICC2.1) was used to examine relative reliability, and the MD was calculated on the basis of standard error of measurement. Results: ICCs for CON KE/KF peak torque measurements were fair to excellent (range, 0.49-0.81). The MD% values for CON KE and KF ranged from 31% to 37% at 60 deg/s and from 34% to 39% at 180 deg/s. ICCs in the ECC mode were good (range, 0.60-0.70), but associated MD% values were high (>50%). There was no systematic error for CON KE/KF and ECC KE strength measurements at 60 deg/s, but systematic error was found for all other measurements. Conclusion: The dynamometer provides a reliable analysis of isokinetic CON knee strength measurements at 60 deg/s in children aged 8 to 10 years. Measurements at 180 deg/s and in the ECC mode were not reliable, indicating a need for more familiarization prior to testing. Clinical Relevance: The MD values may help clinicians to determine whether a change in knee strength is due to error or intervention.
Transplantation of tissue‐engineered cell sheets for stricture prevention after endoscopic submucosal dissection of the oesophagus
Authors: Jonas Eduard , Sjöqvist Sebastian , Elbe Peter , Kanai Nobuo , Enger Jenny , Haas Stephan L , Mohkles‐Barakat Ammar , Okano Teruo , Takagi Ryo , Ohki Takeshi , Yamamoto Masakazu , Kondo Makoto , Markland Katrin , Lim Mei Ling , Yamato Masayuki , Nilsson Magnus , Permert Johan , Blomberg Pontus , Löhr J‐Matthias 
Published: February 2016

Background and objectiveEndoscopic mucosal dissection (ESD) is a treatment option for oesophagus tumours localized to the mucosa enabling en bloc removal of large lesions. The resulting larger mucosal defects have resulted in an increase in the occurrence of post‐treatment strictures. Transplantation of autologous cell sheets, cultured from oral mucosa, has been shown to prevent post‐ESD strictures. The aim of the study was to assess the efficacy and safety of cell sheet transplantation after oesophageal ESD in a Western patient population where reflux‐associated pre‐malignant and malignant conditions predominate.MethodsPatients with Barrett’s oesophagus associated high‐grade dysplasia or early adenocarcinoma where ESD entailed a resection >3 cm in length and ≥75% of the circumference were eligible for treatment under hospital exemption. Cell sheets were cultured from buccal mucosa according to Good Manufacturing Practice and were endoscopically applied to the post‐ESD defect directly after resection. Patients were followed with weekly endoscopy examinations, including confocal laser microscopy, for a total of four weeks.ResultsFive patients were treated. ESD was extensive with resections being circumferential in three patients and 9–10 cm in length in two. The number of transplanted cell sheets ranged from two to six. Three patients developed strictures requiring two to five dilatation sessions.ConclusionsCell sheet transplantation shows to be safe and feasible in a Western population. Results suggest that transplantation has a protective effect on the mucosal defect after ESD, decreasing both the risk for and extent of stricture formation.
The risk of oesophageal adenocarcinoma in a prospectively recruited Barrett’s oesophagus cohort
Authors: Theron BT , Padmanabhan H , Aladin H , Smith P , Campbell E , Nightingale P , Cooper BT , Trudgill NJ 
Published: February 2016

BackgroundVarying rates of oesophageal adenocarcinoma (OAC) complicating Barrett’s oesophagus (BO) have been reported. Recent studies and meta‐analyses suggest a lower incidence, questioning the value of endoscopic surveillance.AimWe aimed to retrospectively examine the rate of OAC, risk factors and causes of death in a prospectively recruited BO cohort.MethodsData from patients with BO from a cohort from 1982–2007 were studied. Patients were subdivided into surveyed, failed to attend surveillance and unfit for surveillance. Standardised mortality ratios (SMR) were calculated for common causes of death. Cox proportional hazards models were used to determine which factors were associated with progression to OAC.ResultsIn total, 671 BO patients (61% male) were studied; 37 (76% male) were diagnosed with OAC. OAC incidence was 0.47% per annum and stable across three decades (1982–1991 0.56%, 1992–2001 0.46%, 2002–2012 0.41% (p  = 0.8)). All‐cause mortality was increased for the whole cohort (SMR 163(95% CI 145–183)). Mortality from OAC appeared higher in patients who failed to attend surveillance (SMR 3216(95% CI 1543–5916)) compared with surveyed (SMR 1753(95% CI 933–2998)) and those unfit for surveillance due to co‐morbidity (SMR 440(95% CI 143–1025)). Multivariable analysis identified low‐grade dysplasia (HR 4.4(95% CI 1.56–12.43), p  = 0.005) and length of BO (HR 1.2(95% (1.1–1.3)), p  < 0.001)) as associated with OAC.ConclusionsProgression to OAC appeared stable over three decades at 0.47% per annum. Patients with BO had a modest increase in all‐cause mortality and a large increase in OAC mortality, particularly if fit for surveillance. Low‐grade dysplasia and the length of the BO segment were associated with developing OAC.
Behaviours and attitudes influencing treatment decisions for menopausal symptoms in five European countries
Authors: Constantine Ginger D , Graham Shelli , Clerinx Cathy , Bernick Brian A , Krassan Mitchell , Mirkin Sebastian , Currie Heather 
Published: February 2016

Objective To assess women’s behaviours and attitudes regarding the treatment of menopausal symptoms in five European countries. Study design Women aged ≥45 years in France, Germany, Italy, Spain, and the United Kingdom participated in an internet survey. Completers were those who reported menopausal symptoms and had treated their symptoms. Women were equally stratified by age (45–54 years, 55–64 years, ≥65 years). Main outcome measures Behaviours, attitudes, and experiences regarding treatment of menopausal symptoms. Results Of 3890 peri- to postmenopausal women screened, 67% experienced symptoms and 54% sought either medical input or some treatment concerning their symptoms. Hot flushes, the most common symptom, decreased with age but remained prevalent after age 64. Roughly 75% of women who sought relief consulted a physician, mostly a gynaecologist or a general practitioner (GP) as in the United Kingdom. The decision to seek treatment was influenced by age, number, and severity of symptoms. Approximately 79% visiting a physician received prescription therapy. Of the women who received non-hormone therapy (HT) treatment instead of HT: patients refused HT (20–44%), physicians did not discuss HT (32–46%), or advised against HT (24–43%). Women in the United Kingdom were most familiar with and favorable to HT. Interest in a new HT (34–50%) was higher than use (19–28%). Conclusions Menopausal symptoms are common, persistent, and bothersome, but many fail to seek treatment. Sources and types of treatment vary among age groups and countries. Education regarding women’s attitudes toward treatment should be provided to those physicians who treat menopausal symptoms in each country.
How Accurate Are Patients at Diagnosing the Cause of Their Knee Pain With the Help of a Web-based Symptom Checker?
Authors: Bisson Leslie J. , Komm Jorden T. , Bernas Geoffrey A. , Fineberg Marc S. , Marzo John M. , Rauh Michael A. , Smolinski Robert J. , Wind William M. 
Published: February 2016

Background: Researching medical information is the third most popular activity online, and there are a variety of web-based symptom checker programs available. Purpose: This study evaluated a patient’s ability to self-diagnose their knee pain from a list of possible diagnoses supplied by an accurate symptom checker. Study Design: Cohort study (diagnosis); Level of evidence, 2. Methods: All patients older than 18 years who presented to the office of 7 different fellowship-trained sports medicine surgeons over an 8-month period with a complaint of knee pain were asked to participate. A web-based symptom checker for knee pain was used; the program has a reported accuracy of 89%. The symptom checker generates a list of potential diagnoses after patients enter symptoms and links each diagnosis to informative content. After exploring the informative content, patients selected all diagnoses they felt could explain their symptoms. Each patient was later examined by a physician who was blinded to the differential generated by the program as well as the patient-selected diagnoses. A blinded third party compared the diagnoses generated by the program with those selected by the patient as well as the diagnoses determined by the physician. The level of matching between the patient-selected diagnoses and the physician’s diagnoses determined the patient’s ability to correctly diagnose their knee pain. Results: There were 163 male and 165 female patients, with a mean age of 48 years (range, 18-76 years). The program generated a mean 6.6 diagnoses (range, 2-15) per patient. Each patient had a mean 1.7 physician diagnoses (range, 1-4). Patients selected a mean 2 diagnoses (range, 1-9). The patient-selected diagnosis matched the physician’s diagnosis 58% of the time. Conclusion: With the aid of an accurate symptom checker, patients were able to correctly identify the cause of their knee pain 58% of the time.
ThPOK represses CXXC5, which induces methylation of histone H3 lysine 9 in Cd40lg promoter by association with SUV39H1: implications in repression of CD40L expression in CD8+ cytotoxic T cells
Authors: Tsuchiya Yukako , Naito Taku , Tenno Mari , Maruyama Mitsuo , Koseki Haruhiko , Taniuchi Ichiro , Naoe Yoshinori 
Published: February 2016

Abstract
CD40 ligand is induced in CD4+ Th cells upon TCR stimulation and provides an activating signal to B cells, making CD40 ligand an important molecule for Th cell function. However, the detailed molecular mechanisms, whereby CD40 ligand becomes expressed on the cell surface in T cells remain unclear. Here, we showed that CD40 ligand expression in CD8+ cytotoxic T cells was suppressed by combined epigenetic regulations in the promoter region of the Cd40lg gene, such as the methylation of CpG dinucleotides, histone H3 lysine 9, histone H3 lysine 27, and histone H4 lysine 20. As the transcription factor Th-inducing pox virus and zinc finger/Kruppel-like factor (encoded by the Zbtb7b gene) is critical in Th cell development, we focused on the role of Th-inducing pox virus and zinc finger/Kruppel-like factor in CD40 ligand expression. We found that CD40 ligand expression is moderately induced by retroviral Thpok transduction into CD8+ cytotoxic T cells, which was accompanied by a reduction of histone H3 lysine 9 methylation and histone H3 lysine 27 methylation in the promoter region of the Cd40lg gene. Th-inducing pox virus and zinc finger/Kruppel-like factor directly inhibited the expression of murine CXXC5, a CXXC-type zinc finger protein that induced histone H3 lysine 9 methylation, in part, through an interaction with the histone-lysine N-methyltransferase SUV39H1. In addition, to inhibit CD40 ligand induction in activated CD4+ T cells by the CXXC5 transgene, our findings indicate that CXXC5 was one of the key molecules contributing to repressing CD40 ligand expression in CD8+ cytotoxic T cells.
The recovery of Tabunio Watershed through enrichment planting using ecologically and economically valuable species in South Kalimantan, Indonesia
Authors: KADIR SYARIFUDDIN , BADARUDDIN BADARUDDIN , NURLINA NURLINA , RIDWAN ICHSAN , RIANAWATI FONNY 
Published: February 2016

Kadir S, Badaruddin, Nurlina, Ridwan I, Rianawaty F. 2016. The recovery of Tabunio Watershed through enrichmentplanting using ecologically and economically valuable species in South Kalimantan, Indonesia. Biodiversitas 17: 140-147. Watershed isa medium system where hydrological-biophysical processes as part of hydrological cycle and social, economic, and cultural events asthe results of human intervention on natural resource occur. The increasing human population results in the increasing need of water thatmay reduce the availability of water, causing environmental degradation. The objectives of this study were to study the characteristics ofTabunio watershed and to give recommendation on the enrichment planting for the watershed recovery. This study used watershedecological area approach and the data were analyzed spatially using GIS. The results showed that based on the characteristics of itscomponents, Tabunio Watershed was categorized as moderately to very highly qualified for recovery. The recovery should be donethrough:(i)Â enrichment planting based on the land capability, land suitability and the legal function of the area, (ii) conservation of soil and water using vegetative and civil engineering methods, and (iii)Â issuance of regulations to increase the watershedâ€™s carrying capacity.
Upstream ORFs are prevalent translational repressors in vertebrates
Authors: Johnstone Timothy G , Bazzini Ariel A , Giraldez Antonio J 
Published: February 2016

AbstractRegulation of gene expression is fundamental in establishing cellular diversity and a target of natural selection. Untranslated mRNA regions (UTRs) are key mediators of post‐transcriptional regulation. Previous studies have predicted thousands of ORFs in 5′ UTRs, the vast majority of which have unknown function. Here, we present a systematic analysis of the translation and function of upstream open reading frames (uORFs) across vertebrates. Using high‐resolution ribosome footprinting, we find that (i) uORFs are prevalent within vertebrate transcriptomes, (ii) the majority show signatures of active translation, and (iii) uORFs act as potent regulators of translation and RNA levels, with a similar magnitude to miRNAs. Reporter experiments reveal clear repression of downstream translation by uORFs/oORFs. uORF number, intercistronic distance, overlap with the CDS, and initiation context most strongly influence translation. Evolution has targeted these features to favor uORFs amenable to regulation over constitutively repressive uORFs/oORFs. Finally, we observe that the regulatory potential of uORFs on individual genes is conserved across species. These results provide insight into the regulatory code within mRNA leader sequences and their capacity to modulate translation across vertebrates.
A General and Robust Framework for Secondary Traits Analysis
Authors: Song Xiaoyu , Ionita-Laza Iuliana , Liu Mengling , Reibman Joan , Wei Ying 
Published: February 2016

AbstractCase–control designs are commonly employed in genetic association studies. In addition to the case–control status, data on secondary traits are often collected. Directly regressing secondary traits on genetic variants from a case–control sample often leads to biased estimation. Several statistical methods have been proposed to address this issue. The inverse probability weighting (IPW) approach and the semiparametric maximum-likelihood (SPML) approach are the most commonly used. A new weighted estimating equation (WEE) approach is proposed to provide unbiased estimation of genetic associations with secondary traits, by combining observed and counterfactual outcomes. Compared to the existing approaches, WEE is more robust against biased sampling and disease model misspecification. We conducted simulations to evaluate the performance of the WEE under various models and sampling schemes. The WEE demonstrated robustness in all scenarios investigated, had appropriate type I error, and was as powerful or more powerful than the IPW and SPML approaches. We applied the WEE to an asthma case–control study to estimate the associations between the thymic stromal lymphopoietin gene and two secondary traits: overweight status and serum IgE level. The WEE identified two SNPs associated with overweight in logistic regression, three SNPs associated with serum IgE levels in linear regression, and an additional four SNPs that were missed in linear regression to be associated with the 75th quantile of IgE in quantile regression. The WEE approach provides a general and robust secondary analysis framework, which complements the existing approaches and should serve as a valuable tool for identifying new associations with secondary traits.
Quantitative Single-Embryo Profile ofDrosophilaGenome Activation and the Dorsal–Ventral Patterning Network
Authors: Sandler Jeremy E , Stathopoulos Angelike 
Published: February 2016

AbstractDuring embryonic development of Drosophila melanogaster, the maternal-to-zygotic transition (MZT) marks a significant and rapid turning point when zygotic transcription begins and control of development is transferred from maternally deposited transcripts. Characterizing the sequential activation of the genome during the MZT requires precise timing and a sensitive assay to measure changes in expression. We utilized the NanoString nCounter instrument, which directly counts messenger RNA transcripts without reverse transcription or amplification, to study &gt;70 genes expressed along the dorsal–ventral (DV) axis of early Drosophila embryos, dividing the MZT into 10 time points. Transcripts were quantified for every gene studied at all time points, providing the first dataset of absolute numbers of transcripts during Drosophila development. We found that gene expression changes quickly during the MZT, with early nuclear cycle 14 (NC14) the most dynamic time for the embryo. twist is one of the most abundant genes in the entire embryo and we use mutants to quantitatively demonstrate how it cooperates with Dorsal to activate transcription and is responsible for some of the rapid changes in transcription observed during early NC14. We also uncovered elements within the gene regulatory network that maintain precise transcript levels for sets of genes that are spatiotemporally cotranscribed within the presumptive mesoderm or dorsal ectoderm. Using these new data, we show that a fine-scale, quantitative analysis of temporal gene expression can provide new insights into developmental biology by uncovering trends in gene networks, including coregulation of target genes and specific temporal input by transcription factors.
ANÁLISE DAS INTERNAÇÕES E DA MORTALIDADE POR DOENÇAS CARDÍACAS ISQUÊMICAS EM IDOSOS NO DISTRITO FEDERAL, NO PERÍODO 2000 A 2012.
Authors: Coelho Marcelo Xavier , Da SILVA Amanda Alves , Vianna LUCY GOMES , Moraes Clayton Franco , De Melo Gislane Ferreira 
Published: February 2016

<p>As doenças do aparelho circulatório estão entre as que apresentam maior morbimortalidade. Dentre elas, a cardiopatia isquêmica é a mais prevalente entre os indivíduos idosos (≥60 anos). O objetivo deste estudo foi investigar a incidência de doença cardíaca isquêmica no Brasil e no Distrito Federal (DF). Os dados foram obtidos na base de dados do Sistema de Informações de Mortalidade e no Sistema de Informações Hospitalares do Sistema Único de Saúde, disponibilizado pelo Departamento de Informática do Sistema Único de Saúde. Foram analisadas as taxas de internação e óbitos por cardiopatia isquêmica em idosos selecionadas destes bancos de dados, no período de 2000 a 2012. A incidência de internações e óbitos nos idosos foi alta no período estudado, tanto no Brasil como no DF, sendo que este último apresentou taxas mais elevadas quando comparadas com a população brasileira. Homens idosos tiveram maior prevalência do que mulheres idosas, tanto no DF quanto no Brasil. Concluiu-se que não houve diminuição nas taxas de internação e de óbitos por doença cardíaca isquêmica no período estudado, em discordância com o citado na literatura. </p>