bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postnatal Wing Morph of Pea Aphids Regulates Hemolymph Trehalose Levels
Authors: Yoshinaga Mayu , Soma Naomi , Kikuta Shingo 
Published: October 2024

ABSTRACTTrehalose, a nonreducing disaccharide composed of two glucose molecules, functions as a critical energy source in various insect tissues and organs and is the predominant sugar component of the hemolymph. The pea aphid, Acyrthosiphon pisum, exhibits higher hemolymph trehalose levels than other insects. However, the dynamics of hemolymph trehalose levels throughout its life stages remain unclear owing to the challenges associated with obtaining hemolymph from these small insects. Therefore, this study was conducted to quantify hemolymph trehalose levels in A. pisum using a fluorescent trehalose sensor (Tre‐C04), which enhances green fluorescent protein fluorescence through the binding of trehalose to a ligand‐binding protein fused to the fluorophore. Trehalose levels were successfully quantified in minimal hemolymph samples from individual aphids, with measurements spanning from the first nymphal stage to the adult stage in both the winged and wingless forms of A. pisum. Hemolymph trehalose levels remained relatively stable throughout the life cycle but exhibited a gradual increase with each developmental stage. Notably, adult winged aphids exhibited significantly higher hemolymph trehalose levels than wingless aphids. Given that wing morph determination occurs early in the nymphal stage, these findings suggest that hemolymph trehalose levels are regulated post‐wing morph development. Further investigation of the expression of genes associated with trehalose metabolism revealed that trehalose phosphate synthase 2 levels were downregulated in early‐stage wingless adults, whereas insulin‐related peptide 5 levels were upregulated in wingless aphids. These findings indicate that A. pisum synthesizes trehalose during the winged adult stage to serve as an energy source for flight.
Diastereoselective Synthesis of Highly Functionalized γ‐Lactams via Ugi Reaction/Michael Addition
Authors: Vidal Herika D. A. , Nunes Paulo S. G. , Martinez Alice K. A. , Januário Marcelo A. P. , Santiago Pedro H. O. , Ellena Javier , Corrêa Arlene G. 
Published: October 2024

AbstractThe γ‐lactam ring is a prominent feature in medicinal chemistry, and its synthesis has garnered significant interest due to its valuable properties. Among the γ‐lactams, 2‐oxopyrrolidine‐3‐carbonitrile derivatives stand out as versatile synthons that can be readily transformed into a variety of other functional groups. In this work, we successfully synthesized highly functionalized 3‐cyano‐2‐pyrrolidinones with moderate to good overall yields using the Ugi reaction followed by intramolecular Michael addition. The process demonstrated excellent diastereoselectivity and showed good tolerance to a range of isonitriles and carbonyl compounds.
Idiosyncratic pupil regulation in autistic children
Authors: Bleimeister Isabel H. , Avni Inbar , Granovetter Michael C. , Meiri Gal , Ilan Michal , Michaelovski Analya , Menashe Idan , Behrmann Marlene , Dinstein Ilan 
Published: October 2024

AbstractRecent neuroimaging and eye‐tracking studies have suggested that children with autism exhibit more variable and idiosyncratic brain responses and eye movements than typically developing (TD) children. Here, we extended this research to pupillometry recordings. We successfully acquired pupillometry recordings from 111 children (74 with autism), 4.5‐years‐old on average, who viewed three 90 s movies, twice. We extracted their pupillary time‐course for each movie, capturing their stimulus evoked pupillary responses. We then computed the correlation between the time‐course of each child and those of all others in their group as well as between each autistic child and all children in the TD group. This yielded an average inter‐subject correlation value per child, representing how similar their pupillary responses were to all others in their group or the comparison group. Children with autism exhibited significantly weaker inter‐subject correlations than TD children in all comparisons. These differences were independent of previously reported differences in gaze inter‐subject correlations and were largest in responses to a naturalistic movie containing footage of a social interaction between two TD children. The results demonstrate the utility of measuring the idiosyncrasy of pupil regulation, which can be performed with passive viewing of movies even by young children with co‐occurring intellectual disability. These findings reveal that a considerable number of children with autism have significantly less stable, idiosyncratic pupil regulation than TD children, indicative of more variable, weakly regulated, underlying neural activity.
Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution
Authors: Song Chenyang , Guo Jian , Wang Yuhan , Xiang Hongying , Yang Yufeng 
Published: October 2024

ABSTRACTGlucose sensors are essential tools for monitoring blood glucose concentration in diabetic patients. In recent years, with the increasing number of individuals suffering from diabetes, blood glucose monitoring has become extremely necessary, which expedites the iteration and upgrade of glucose sensors greatly. Currently, two main types of glucose sensors are available for blood glucose testing: enzyme‐based glucose sensor (EBGS) and enzyme‐free glucose sensor (EFGS). For EBGS, several progresses have been made to comprehensively improve detection performance, ranging from enhancing enzyme activity, thermostability, and electron transfer properties, to introducing new materials with superior properties. For EFGS, more and more new metallic materials and their oxides are being applied to further optimize its blood glucose monitoring. Here the latest progress of electrochemical glucose sensors, their manufacturing methods, electrode materials, electrochemical parameters, and applications were summarized, the development glucose sensors with various noninvasive sampling modes were also compared.
Stakeholder and customer engagement in circular economy ecosystems: A systematic literature review and research agenda
Authors: Tabas Abdollah Mohammadparst , Rehman Mohsin Abdur , Khitous Fatima , Urbinati Andrea 
Published: October 2024

AbstractIn the dynamic landscape of circular economy ecosystems, the engagement between stakeholders and customers plays a crucial role in the transition toward circular economy. On the one side, stakeholders collectively engage in generating value for customers, while on the other, customers benefit from this value. This literature especially highlights the collaborative and collective efforts of stakeholders working together to create value for customers and how these efforts allow for the effectiveness of circular economy ecosystems. However, existing literature lacks a holistic understanding of how stakeholders and customers engage in circular economy ecosystems with each other. Our research takes stock of this research gap. In particular, our literature review aims to fill this gap by systematically selecting, reviewing, and synthesizing 77 articles sourced from Scopus and Web of Science databases. Our analysis shows the importance of the configuration of strategies for value creation and exchange, and ecosystem dynamics, including the drivers and barriers that affect the effectiveness of stakeholder and customer engagement in circular economy ecosystems. Moreover, the findings highlight the importance of raising awareness for customer engagement with circular products and services. Finally, we present an integrated framework that encompasses stakeholder and customer engagement along with the theoretical lenses that can be used to deepen our understanding of the phenomenon. Ultimately, the proposed framework outlines future research directions to advance stakeholder and customer engagement in circular economy ecosystems.
Digital transformation and corporate green innovation: An affordance theory perspective
Authors: Sun Zhe , Zhao Liang , Mehrotra Ankit , Salam Muhammad Asif , Yaqub Muhammad Zafar 
Published: October 2024

AbstractThe rise of cutting‐edge technologies motivates corporations to undertake and carry forward green innovation, aiding economic development by maintaining environmental sustainability. Using A‐share listed firms from 2013 to 2022 as the research sample, the paper empirically examines the impact of digital transformation on corporate green innovation from an affordance perspective. The findings suggest that the higher the level of digital transformation, backed by accumulative and variational affordances, in a firm, the more conducive it is to corporate green innovation as it enables the homogenization, recombination, and transformation of existing information related to green environmental protection and low‐carbon energy efficiency, facilitating firms to achieve targeted and breakthrough green innovations. The analysis of the moderating effects of public environmental concern, economic policy uncertainty, and regional innovation readiness suggested that public environmental concern and regional innovation readiness positively moderate the relationship between digital transformation and corporate green innovation, while economic policy uncertainty perception negatively moderates this relationship. Heterogeneity analyses suggest that digital transformation positively affects corporate green innovation within labor‐intensive firms and state‐owned enterprises. The study contributes to the literature by enhancing our understanding of the affordance theory in the domain of digital transformation. By investigating the key organizational and institutional affordances – economic policy uncertainty perception, public environmental concern, and regional innovation readiness – the research provides valuable insight to policymakers in fostering green innovation.
Analytical and clinical validation of a NGS panel in detecting targetable variants from ctDNA of metastatic NSCLC patients
Authors: Fan Feifei , Jiang Guozhong , Lv Juan , Wang Hongmin , Li Wenjie , Liu Chenglin , Zhao Yu , Zhang Zhou , Du Haiwei , Zhang Zhihong , Li Xiangnan , Li Wen‐cai 
Published: October 2024

AbstractBackgroundCirculating tumor DNA (ctDNA) has emerged as a promising biomarker for noninvasive cancer diagnostics, particularly in the context of metastatic non‐small‐cell lung cancer (NSCLC). Detecting targetable variants through ctDNA analysis offers the potential to guide treatment decisions, especially in cases where tissue samples are insufficient or unavailable.MethodIn this study, we developed and validated a next‐generation sequencing panel targeting 101 cancer‐related genes (101‐test) to detect somatic variants in ctDNA from a large cohort of Chinese patients with metastatic NSCLC. The performance of the 101‐test was assessed by evaluating its limit of detection (LOD), accuracy, and precision in identifying molecular variants. Additionally, the concordance between ctDNA and tissue samples for detecting targetable variants was analyzed in 904 patients.ResultsThe 101‐test demonstrated a LOD of 0.38% for single‐nucleotide variants (SNVs), 0.33% for insertions and deletions (InDels), and 0.33% for fusions. Sensitivity was 98.3% for SNVs, 100% for InDels, and 100% for fusions when compared to digital droplet PCR (ddPCR)/breakpoint PCR reference methods. The by‐variant sensitivity for somatic variants was 97.5%, with a specificity of 99.9% between tumor‐only and tumor‐normal analyses. In a real‐world cohort, the concordance between ctDNA and tissue samples for identifying targetable variants was 72.2% (457/633). Notably, the EGFR S768I variant, recently recommended by clinical guidelines, achieved an 80% concordance rate. Furthermore, 4.3% of patients (27/633) with targetable variants were identified exclusively through ctDNA testing.ConclusionThe ctDNA‐based 101‐test is a highly sensitive and specific tool for detecting targetable variants in metastatic NSCLC, particularly in cases with insufficient tissue samples. The findings support the use of ctDNA testing as a reliable and complementary method to traditional tissue‐based molecular analysis, enhancing the precision of treatment strategies for NSCLC patients.
TRPM8 overexpression suppresses hepatocellular carcinoma progression and improves survival by modulating the RTP3/STAT3 pathway
Authors: Chen Lichan , Xu Nansong , Gou DongMei , Song Jianning , Zhou Mingqin , Zhang Yajun , Zhang Haohua , Zhu Liwen , Huang Weihong , Zhu Yue , Gao Cheng , Gu Dayong , Xu Yong , Zhou Hongzhong 
Published: October 2024

AbstractBackground and AimsHepatocellular carcinoma (HCC) is a malignant tumour associated with high morbidity and mortality rates worldwide. Recently, TRPM8 was reported to play an important role in tumour progression. However, the precise role of TRPM8 in HCC remains unclear. In this study, we explored the expression levels, molecular functions and underlying mechanisms of TRPM8 in HCC.MethodsTissue samples were used to analyse the expression of TRPM8 to assess its diagnostic value for prognosis. Cell Counting Kit‐8, EdU and colony formation assays were performed to evaluate the effects of TRPM8 on cell proliferation, whereas the Transwell assay was used to assess cell migration and invasion. The role of TRPM8 in vivo was evaluated using a mouse subcutaneous xenograft tumour model. We performed PPI network analyses to understand the possible mechanisms of TRPM8 action.ResultsTRPM8 expression was decreased in HCC tissues and was correlated with histological grade and poor patient prognosis. Functionally, TRPM8 repressed the proliferation and metastasis of HCC cells both in vitro and in vivo by modulating the RTP3/STAT3 signalling pathway.ConclusionOur findings underscore the critical role of the TRPM8‐RTP3‐STAT3 axis in maintaining the malignant progression of HCC. Moreover, our study demonstrates that AD80 is involved in anti‐tumour processes by upregulating the expression of TRPM8.
Parents' Distress and Demands for Children With Cancer‐Related Cognitive Impairment in Western China: A Qualitative Study
Authors: Liu Tao , Xu LiFang , Shen Yuqing , Chen Zefang , Yao Juan , Mo Lin 
Published: October 2024

ABSTRACTBackgroudCancer‐related cognitive impairment is one of the common complications in children with cancer, but our understanding of their experience with cognitive deficits remains limited. From the perspective of parents, this study aimed to explore the distress and demands faced by children with cancer‐related cognitive impairment, to provide references for developing targeted intervention strategies for cancer children.MethodsWe used a purposeful sampling method to conduct semi‐structured interviews with the parents of 18 children with cancer‐related cognitive impairment. The transcripts were analyzed using Colaizzi's method.ResultsThree categories and 11 subcategories were obtained from the data analysis, including diverse levels of cognitive impairment in children (speech communication difficulties, impaired executive function, attention deficit, and intellectual disability), persistent negative emotions (anxiety and worry, complaints and resentment, negative avoidance and positive experiences after psychological adjustment), multiple needs (need for disease information, need for professional management, and an urgent need for more external support).ConclusionParents of children with cancer‐related cognitive impairment face a significant psychological burden, coupled with confusion and numerous inquiries regarding the symptoms and management of their children's cognitive impairment. It is imperative for medical professionals to strengthen the dissemination of information related to cancer‐associated cognitive impairments, while promptly recognizing and intervening in related symptoms. Rational allocation of resources, establishment of targeted support systems, and enhancement of social acceptance may be the key points that policymakers could consider. These efforts hold immense significance, as they play a pivotal role in elevating cognitive capabilities and enhancing the overall quality of life for children with cancer.
Polyphenolic Profile and In vitro, In Silico Study of Stem Extracts from J. Maritimus (Juncaceae) Harvested from Eastern Algeria as Potential Anti‐Inflammatory and Antioxidant Agents
Authors: Zeroual Samir , Daoud Ismail , Gaouaoui Randa , Kherachi Rania 
Published: October 2024

AbstractJuncus maritimusan extremophilous microorganism speciesknown for its medicinal properties was collected in the Biskra region with the aim of its valorization. The volume size of polyphenols, flavonoids and condensed tannins was performed using the Folin‐Ciocalteu method, aluminum trichloride and vanillin respectively. The volume of Polyphenols was 127.73±0.20 μg EAG/mg ES, the volume offlavonoids was 16.42±0.42 μg EQ/mg ES, and the volume ofcondensed tannins was 10.10±0.35 μg EC/mg ES. The results of the antioxidant activity tests using the DPPH and ABTS methods reveal that the ethyl acetate extract had the highest activity in both tests. The results of the in vitro anti‐inflammatory activity, using the BSA protein denaturation assay, showed that the percentage of denaturation inhibition wasproportional to the concentration of the extract. At a concentration of 5 mg/mL, the inhibition percentage of the extract were 82.03 % and 80.23 %, respectively,which wereclose tothose of theanti‐inflammatory drug Diclofenac.Furthermore, molecular docking simulations indicated that Berberine has high binding affinity to the targets COX‐2 and PLA‐2 In fact,bioisosteric replacement is being usedto discover new analogs of Berberine. Finally, ADME‐Tox predictions demonstrated that this compound and its analogs were not hepatotoxic. This result may lead to the selection ofBerberine and its analogs as active compounds with anti‐inflammatory and antioxidant effects.
Human 8‐Oxoguanine Glycosylase OGG1 Cleaves Abasic Sites and Covalently Conjugates to 3′‐DNA Termini via Cysteine and Histidine Addition
Authors: Bryan Cameron , Yang Kun 
Published: October 2024

Abstract8‐Oxoguanine glycosylase 1 (OGG1) repairs the major oxidative DNA damage, 8‐oxo‐2′‐deoxyguanosine. It has been reported that OGG1 incises the most frequently formed DNA lesion, apurinic/apyrimidinic (AP) site, and in the process a stable DNA‐OGG1 cross‐link is formed. However, the chemical structure of the adduct is not characterized. Here, we report that DNA‐OGG1 cross‐links result from cysteine and histidine addition to incised AP sites at 3′‐DNA termini.
Impact of Flame Conditions on the Pd‐O Structure and Methane Oxidation Activity over Ceria Support
Authors: Wang Can , Najimu Musa , Ko Ben , Sasmaz Erdem 
Published: October 2024

AbstractIn this work, we employed flame spray pyrolysis (FSP), a high‐temperature synthesis method, to control the formation of Pd structures on the CeO2 support. Multiple types of Pd structures deposited on CeO2 are observed on FSP‐made samples. Our results show that the oxidizing environment during FSP synthesis facilitates the formation of incorporated Pd2+ structures, along with highly dispersed Pd2+, Pd0 nanoparticles, and Pd° clusters formed under the reducing synthesis condition. Notably, these Pd2+ species remained stable at temperatures up to 400 °C. The catalysts containing both highly dispersed Pd2+ nanoparticles and incorporated Pd2+ species demonstrated superior methane oxidation activity, with higher turnover frequencies than those containing only one type of Pd2+ structure. However, hydrothermal pretreatment in the presence of water vapor led to partial deactivation, likely due to structural alterations in the Pd species or the interaction with the CeO2 support, which reduced the stability and effectiveness of the active sites. This study underscores the importance of both highly dispersed and incorporated Pd2+ species in enhancing catalytic performance and highlights the challenges posed by water‐induced deactivation in practical applications.
Application of Deep Neural Network Technology for Multi‐scale CFD Modeling in Porous Media
Authors: Li Jiaxu , Liu Tingting , Jia Shuqin , Xu Chao , Fan Tingxuan , Huai Ying 
Published: October 2024

AbstractSystem‐scale computational fluid dynamics (CFD) simulations in chemical and process engineering remain limited owing to the complexity of integrating the results obtained at different scales. The present study addresses this issue by correlating the flow behaviors calculated by CFD in porous media at the micro‐scale and the macro‐scale using deep neural network (DNN) technology. The DNN model is trained using a dataset constructed from the results obtained for a large number of particle‐scale CFD simulations that are coupled to macroscopic governing equations. Comparisons with experimental results obtained with a packed bed show that the proposed CFD‐DNN method provides predictions of pressure drop with an accuracy that is 28% greater than that of a method based on the Ergun equation.
Fortuitous Enantiomeric Self‐Rectification of an Unreported Partial Racemisation in the Synthesis of a Chiral Phosphoric Acid: A Warning to Practitioners
Authors: Lancaster Ben M. J. , White Andrew J. P. , Christopher Braddock D. 
Published: October 2024

AbstractRacemisation without Consequence: MOM deprotection during a routine synthesis of phosphoric acid 1 promoted partial racemisation of the product BINOL 5. Surprisingly, however, after phosphorylation of the partially racemised BINOL, enantiopure acid 1 was isolated. Further inspection revealed that during phosphorylation, unhydrolysed racemic phosphorochloridate 6 precipitated, thus restoring homochirality in the product phosphoric acid 1. Moreover, MOM deprotection partial racemisation was avoided by conducting reactions at lower temperatures for no longer than the required deprotection time, and/or by application of other deprotection conditions from the literature.