bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu
Authors: Nemati Sorour , Rezabakhsh Aysa , Khoshfetrat Ali Baradar , Nourazarian Alireza , Biray Avci Çığır , Goker Bagca Bakiye , Alizadeh Sardroud Hamed , Khaksar Majid , Ahmadi Mahdi , Delkhosh Aref , Sokullu Emel , Rahbarghazi Reza 
Published: August 2017

AbstractUp to present, many advantages have been achieved in the field of cell‐based therapies by applying sophisticated methodologies and delivery approaches. Microcapsules are capable to provide safe microenvironment for cells during transplantation in a simulated physiological 3D milieu. Here, we aimed to investigate the effect of alginate‐gelatin encapsulation on angiogenic behavior of human endothelial cells over a period of 5 days. Human umbilical vein endothelial cells were encapsulated by alginate‐gelatin substrate and incubated for 5 days. MTT and autophagy PCR array analysis were used to monitor cell survival rate. For in vitro angiogenesis analysis, cell distribution of Tie‐1, Tie‐2, VEGFR‐1, and VEGFR‐2 were detected by ELISA. In addition to in vitro tubulogenesis assay, we monitored the expression of VE‐cadherin by Western blotting. The migration capacity of encapsulated HUVECs was studied by measuring MMP‐2 and MMP‐9 via gelatin zymography. The in vivo angiogenic potential of encapsulated HUVECs was analyzed in immune‐compromised mouse implant model during 7 days post‐transplantation. We demonstrated that encapsulation promoted HUVECs cell survival and proliferation. Compared to control, no significant differences were observed in autophagic status of encapsulated cells (p > 0.05). The level of Tie‐1, Tie‐2, VEGFR‐1, and VEGFR‐2 were increased, but did not reach to significant levels. Encapsulation decreased MMP‐2, ‐9 activity and increased the VE‐cadherin level in enclosed cells (p < 0.05). Moreover, an enhanced in vivo angiogenic response of encapsulated HUVECs was evident as compared to non‐capsulated cells (p < 0.05). These observations suggest that alginate‐gelatin encapsulation can induce angiogenic response in in vivo and in vitro conditions.
Incorporating LsrK AI‐2 quorum quenching capability in a functionalized biopolymer capsule
Authors: Rhoads Melissa K. , Hauk Pricila , Terrell Jessica , Tsao Chen‐Yu , Oh Hyuntaek , Raghavan Srinivasa R. , Mansy Sheref S. , Payne Gregory F. , Bentley William E. 
Published: August 2017

AbstractAntibacterial resistance is an issue of increasing severity as current antibiotics are losing their effectiveness and fewer antibiotics are being developed. New methods for combating bacterial virulence are required. Modulating molecular communication among bacteria can alter phenotype, including attachment to epithelia, biofilm formation, and even toxin production. Intercepting and modulating communication networks provide a means to attenuate virulence without directly interacting with the bacteria of interest. In this work, we target communication mediated by the quorum sensing (QS) bacterial autoinducer‐2, AI‐2. We have assembled a capsule of biological polymers alginate and chitosan, attached an AI‐2 processing kinase, LsrK, and provided substrate, ATP, for enzymatic alteration of AI‐2 in culture fluids. Correspondingly, AI‐2 mediated QS activity is diminished. All components of this system are “biofabricated”—they are biologically derived and their assembly is accomplished using biological means. Initially, component quantities and kinetics were tested as assembled in microtiter plates. Subsequently, the identical components and assembly means were used to create the “artificial cell” capsules. The functionalized capsules, when introduced into populations of bacteria, alter the dynamics of the AI‐2 bacterial communication, attenuating QS activated phenotypes. We envision the assembly of these and other capsules or similar materials, as means to alter QS activity in a biologically compatible manner and in many environments, including in humans.
Virtual protein purification: A simple exercise to introduce ph as a parameter that effects ion exchange chromatography
Authors: Clark Daniel D. , Edwards Daniel J. 
Published: August 2017

AbstractThis article describes a simple exercise using a free, easy‐to‐use, established online program. The exercise helps to reinforce protein purification concepts and introduces undergraduates to pH as a parameter that affects anion‐exchange chromatography. The exercise was tested with biochemistry majors at California State University‐Chico. Given the versatility of the program, this work is also a model for instructors that wish to develop their own exercise to help teach other protein purification techniques. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(1):91–97, 2018.
Hippocampal volume, social interactions, and the expression of the normal repertoire of resident–intruder behavior
Authors: Kalman Eszter , Keay Kevin A. 
Published: August 2017

AbstractIntroductionReduced hippocampal volumes are reported in individuals with disrupted emotional coping behaviors in both human clinical conditions and in experimental animal models of these populations. In a number of experimental animal models, it has been shown that social interactions can promote resilience and buffer the negative neural consequences of stimuli that disrupt effective coping.MethodsHippocampal and dentate gyrus volumes were calculated in 54 male Sprague Dawley rats; (1) single housed (n = 12), (2) single housed and exposed to daily 6‐min social interactions testing in a resident–intruder paradigm (n = 11); (3) group housed (n = 12); (4) single housed and sham injured (n = 12); (5) single housed, sham injured, and social interactions tested (n = 7).ResultsWe present data which shows that even a brief daily exposure to a conspecific in resident–intruder social interactions test is sufficient to prevent the reduction in hippocampal volume triggered by single housing.ConclusionWhen considered with previously published data, these findings suggest that the expression of the full repertoire of social, nonsocial, dominance, and submissive behaviors in response to the physical presence of an intruder in the home cage plays a significant role in this maintenance of hippocampal volume.
Leadership in community mutual support groups for mental health: A qualitative case study from the leaders' perspective
Authors: Gammage Rebecca J. , Foster Juliet L. 
Published: August 2017

AbstractThis study explores the experience of leadership of member‐led community‐based mental health mutual support groups. Recent research has documented factors that affect these groups, including leader well‐being, but little is known about the experience of leadership at the individual level. We aimed to understand more about the experience of leadership and how leading members conceptualise their roles. Individual semi‐structured interviews were conducted with 14 leaders of a community mental health mutual support group in England and thematically analysed. Three themes were identified through which leading members conceptualised their roles and group dynamics: (a) “It's a family”; (b) professional values; and (c) working as a team. These knowledge frameworks appeared to influence leaders' well‐being and conceptualisations of their role. The potential impact of this on group stability is discussed. Recommendations are made that group and individual‐level processes be considered together in future research in mental health mutual support contexts due to their interconnected nature.
Porphyrin‐Hyperbranched Copolymer Films as Recyclable Photooxidation Catalysts for 1,5‐Dihydroxynaphthalene
Authors: Sun Kaifang , Guo Lintong , Cai Cheng , Hou Zongsheng , Ren Qizhi 
Published: August 2017

AbstractNovel and stable films were easily assembled at the water–chloroform interface by noncovalent interaction between meso‐tetrakis(4‐sulfonatophenyl)porphyrins (H2TPPS, FeTPPS, and ZnTPPS) and polypeptide‐containing hyperbranched copolymers of various molecular weights. These films could be used as recyclable heterogeneous photocatalysts for the oxidation of 1,5‐dihydroxynaphthalene. Conversion yields of 91 and 88 % were obtained for H2TPPS and FeTPPS, respectively, and these values were much higher than those obtained for the corresponding porphyrin systems without polymers. These films could be easily utilized over 10 catalytic runs with only a slight decrease in the catalytic activity and low porphyrin loss. No separation or purification of either the catalyst or product was necessary, which proved that these porphyrin–hyperbranched copolymer films could be efficient, robust, and recyclable photooxidation catalysts.
Multi‐Step Consecutive Photo‐Chlorination of 1,2‐Dichloroethane: Kinetics and Reactive Distillation Experiment
Authors: Chen Xian , Xu Ge , Li Yunpeng , Chen Wei , Tang Jihai , Fei Zhaoyang , Cui Mifen , Guo Haiwei , Qiao Xu 
Published: August 2017

AbstractThe development and validation of a novel kinetic model for the chlorination of 1,2‐dichloroethane to ultimately hexachloroethane catalyzed by blue light are presented. A factor (Xm) related to the chlorination depth was introduced into the kinetics model, and the results showed that the dynamic features of the photo‐chlorination are better predicted by the modified model than by the conventional one. Furthermore, to enhance the selectivity towards tetrachloroethane and pentachloroethane, a novel reactive distillation with side‐reactor configuration (SRC) for the photo‐chlorination process was proposed. In order to demonstrate the technical feasibility of the process, semi‐batch SRC experiments on the laboratory scale were carried out. Up to 96.5 wt % of tetrachloroethane and pentachloroethane with molar ratios varying from 7 to 0.7 over time were obtained in the bottom product.
Investigating Intracellular Localisation and Cytotoxicity Trends for Neutral and Cationic Iridium Tetrazolato Complexes in Live Cells
Authors: Caporale Chiara , Bader Christie A. , Sorvina Alexandra , MaGee Karen D. M. , Skelton Brian W. , Gillam Todd A. , Wright Phillip J. , Raiteri Paolo , Stagni Stefano , Morrison Janna L. , Plush Sally E. , Brooks Douglas A. , Massi Massimiliano 
Published: August 2017

AbstractA family of five neutral cyclometalated iridium(III) tetrazolato complexes and their methylated cationic analogues have been synthesised and characterised. The complexes are distinguished by variations of the substituents or degree of π conjugation on either the phenylpyridine or tetrazolato ligands. The photophysical properties of these species have been evaluated in organic and aqueous media, revealing predominantly a solvatochromic emission originating from mixed metal‐to‐ligand and ligand‐to‐ligand charge transfer excited states of triplet multiplicity. These emissions are characterised by typically long excited‐state lifetimes (∼hundreds of ns), and quantum yields around 5–10 % in aqueous media. Methylation of the complexes caused a systematic red‐shift of the emission profiles. The behaviour and the effects of the different complexes were then examined in cells. The neutral species localised mostly in the endoplasmic reticulum and lipid droplets, whereas the majority of the cationic complexes localised in the mitochondria. The amount of complexes found within cells does not depend on lipophilicity, which potentially suggests diverse uptake mechanisms. Methylated analogues were found to be more cytotoxic compared to the neutral species, a behaviour that might to be linked to a combination of uptake and intracellular localisation.
Cyclopalladation in the Periphery of a NHC Ligand as the Crucial Step in the Synthesis of Highly Active Suzuki–Miyaura Cross‐Coupling Catalysts
Authors: Fizia Agnes , Gaffga Maximilian , Lang Johannes , Sun Yu , Niedner‐Schatteburg Gereon , Thiel Werner R. 
Published: August 2017

AbstractStarting from 2,4‐dichloropyrimidine, 4‐(2‐dialkylamino)pyrimidinyl functionalized mesitylimidazolium chlorides are accessible in a five‐step reaction sequence. Two routes leading to palladium NHC complexes derived from these ligands have been worked out: By transmetalation with the corresponding NHC‐AgCl complexes, C,N‐coordinated palladium(II) complexes can be obtained. Treatment of palladium dichloride with the imidazolium salts in pyridine and in the presence of K2CO3 gives cyclometalated and thus C,C‐coordinated compounds. The reactivities of all these compounds were investigated in detail as well as their performance in the catalytic Suzuki–Miyaura cross‐coupling reaction. It turned out that the C,C‐coordinated derivatives exhibit high catalytic activities in the coupling of arylboronic acids with aryl chlorides, which is consistent with the generally accepted mechanistic ideas on substrate activation.
Homochiral Emissive Λ8‐ and Δ8‐[Ir8Pd4]16+ Supramolecular Cages
Authors: Rota Martir Diego , Escudero Daniel , Jacquemin Denis , Cordes David B. , Slawin Alexandra M. Z. , Fruchtl Herbert A. , Warriner Stuart L. , Zysman‐Colman Eli 
Published: August 2017

AbstractSynthetic self‐assembly is a powerful technique for the bottom‐up construction of discrete and well‐defined polyhedral nanostructures resembling the spherical shape of large biological systems. In recent years, numerous Archimedean‐shaped coordination cages have been reported based on the assembly of bent monodentate organic ligands containing two or more distal pyridyl rings and square‐planar PdII ions. The formation of photoactive PdII metallamacrocycles and cages, however, remain rare. Here we report the first examples of emissive and homochiral supramolecular cages of the form [Ir8Pd4]16+. These cages provide a suitably sized cavity to host large guest molecules. Importantly, encapsulation and energy transfer have been observed between the blue‐emitting NBu4[Ir(dFppy)2(CN)2] guest and the red‐emitting Δ8‐[Ir8Pd4]16+ cage.
Possible Case of Halogen Bond‐Driven Self‐Disproportionation of Enantiomers (SDE) via Achiral Chromatography
Authors: Terada Shumpei , Hirai Motohiro , Honzawa Ayaka , Kitagawa Osamu , Kamizela Angelika , Wzorek Alicja , Soloshonok Vadim A. 
Published: August 2017

AbstractThe major breakthrough reported in this work is the discovery of likely halogen bond‐driven self‐disproportionation of enantiomers (SDE). Taking into account that the halogen‐bonding interactions can be rationally designed and can match, or even exceed, the strength of the more familiar hydrogen bond, this discovery clearly opens an unexpected new direction of research in the areas of molecular chirality and the SDE phenomenon.
A Guide for the Design of Functional Polyaromatic Organophosphorus Materials
Authors: Hindenberg Philip , López‐Andarias Alicia , Rominger Frank , de Cózar Abel , Romero‐Nieto Carlos 
Published: August 2017

AbstractInvited for the cover of this issue are groups of Abel de Cózar from the University of País Vasco, and Carlos Romero‐Nieto from the Ruprecht‐Karls‐University in Heidelberg. The image depicts the many different properties that can be achieved with different fused phosphorus heterocycles. Read the full text of the article at 10.1002/chem.201701649.
Application of the Pentafluorosulfanyl Group as a Bioisosteric Replacement
Authors: Sowaileh Munia F. , Hazlitt Robert A. , Colby David A. 
Published: August 2017

AbstractThe success of fluorinated molecules in drug design has led medicinal chemists to search for new fluorine‐containing substituents. A major recently developed group is the pentafluorosulfanyl group. This group is stable under physiological conditions and displays unique physical and chemical properties. There are currently few synthetic methods to install the SF5 group, yet efforts to integrate this group into lead optimization continue unabated. Typically, the SF5 group has been used as a replacement for trifluoromethyl, tert‐butyl, halogen, or nitro groups. In this review, the use of the SF5 group as a bioisosteric replacement for each of these three functionalities is compared and contrasted across various groups of biologically active molecules. The organization and presentation of these data should be instructive to medicinal chemists considering to design synthetic strategies to access SF5‐substituted molecules.
Different Color Emissive Copper Nanoclusters for Cancer Cell Imaging
Authors: Basu Kingshuk , Gayen Kousik , Mitra Tulika , Baral Abhishek , Roy Sib Sankar , Banerjee Arindam 
Published: August 2017

AbstractDifferent sized copper nanoclusters (CuNCs) have been synthesized in water from the same metal precursor and stabilizing agent, only by altering the reducing agent, temperature and pH of the medium. As‐synthesized clusters were thoroughly characterized by fluorescent spectroscopy, matrix‐assisted laser desorption ionization (MALDI) mass spectrometry, X‐ray photoelectron spectroscopy (XPS), Fourier transform infra‐red spectroscopy (FT‐IR) and transmission electron microscopy (TEM). Interestingly, the emissive color of nanoclusters has been successfully tuned from blue to orange‐red in aqueous media and four different color emitting clusters have been found, namely, blue, cyan, green and orange‐red. Orange‐red emitting CuNC is associated with a large Stokes shift of 283 nm and it is non‐cytotoxic in nature. Fluorophores with such high Stokes shift are highly advantageous for modern microscopic techniques; in this study, the as‐synthesized orange‐red emitting clusters have been employed for imaging of cancer cells to check their ability for cell imaging for future biomedical applications.
Luseogliflozin, an SGLT2 Inhibitor, in Japanese Patients With Mild/Moderate Hepatic Impairment: A Pharmacokinetic Study
Authors: Samukawa Yoshishige , Sata Michio , Furihata Kenichi , Ito Toshifumi , Ueda Naohiko , Ochiai Hidekazu , Sakai Soichi , Kumagai Yuji 
Published: August 2017

AbstractThis open‐label, parallel‐group study evaluated the effect of mild and moderate hepatic impairment on the pharmacokinetics of a single dose of luseogliflozin in Japanese subjects. Thirteen subjects with hepatic impairment (mild, n = 8; moderate, n = 5) and 6 healthy subjects received a single 5‐mg dose of luseogliflozin. Serial blood sampling over 72 hours and 24‐hour urine collection were done for pharmacokinetic analysis of luseogliflozin and its metabolites and to measure pharmacokinetic and pharmacodynamic parameters, respectively. Demographic characteristics were similar at baseline for both groups. Geometric mean ratios of maximum plasma concentration (Cmax) and area under the plasma concentration–time curve from time zero to infinity (AUCinf [90%CI]) of unchanged luseogliflozin were 1.02 (0.790–1.32) and 0.774 (0.580–1.03), respectively, on comparing patients with hepatic impairment with healthy subjects, and 0.939 (0.752–1.17) and 1.00 (0.780–1.28), respectively, in subjects with mild and moderate hepatic impairment. Although mean plasma concentrations of metabolites were slightly higher in patients with hepatic impairment versus healthy subjects, their time‐course plasma concentrations were very low compared with those of unchanged luseogliflozin. Single‐dose luseogliflozin 5 mg was well tolerated by study participants, indicating luseogliflozin dose adjustment is not necessary in patients with mild and moderate hepatic impairment.
Evaluation of Potential Drug–Drug Interaction Between Delayed‐Release Dimethyl Fumarate and a Commonly Used Oral Contraceptive (Norgestimate/Ethinyl Estradiol) in Healthy Women
Authors: Zhu Bing , Nestorov Ivan , Zhao Guolin , Meka Venkata , Leahy Mark , Kam Jeanelle , Sheikh Sarah I. 
Published: August 2017

AbstractDelayed‐release dimethyl fumarate (DMF) is an oral therapy for relapsing multiple sclerosis with anti‐inflammatory and neuroprotective properties. This 2‐period crossover study was conducted to evaluate the potential for drug–drug interaction between DMF (240 mg twice daily) and a combined oral contraceptive (OC; norgestimate 250 μg, ethinyl estradiol 35 μg). Forty‐six healthy women were enrolled; 32 completed the study. After the lead‐in period (OC alone), 41 eligible participants were randomized 1:1 to sequence 1 (OC and DMF coadministration in period 1; OC alone in period 2) or sequence 2 (regimens reversed). Mean concentration profiles of plasma norelgestromin (primary metabolite of norgestimate) and ethinyl estradiol were superimposable following OC alone and OC coadministered with DMF, with 90% confidence intervals of geometric mean ratios for area under the plasma concentration–time curve over the dosing interval and peak plasma concentration contained within the 0.8–1.25 range. Low serum progesterone levels during combined treatment confirmed suppression of ovulation. The pharmacokinetics of DMF (measured via its primary active metabolite, monomethyl fumarate) were consistent with historical data when DMF was administered alone. No new safety concerns were identified. These results suggest that norgestimate/ethinyl estradiol–based OCs may be used with DMF without dose modification.
A collaborative care skills workshop for carers: Can it be delivered in 1 day?
Authors: Jenkins Paul E. , Bues Sonia , Cottrell Julie , Hawkins Joel , Pinder Laura , Price Susan , Stewart Anne 
Published: August 2017

Carers of individuals with eating disorders (EDs) report high levels of burden and distress and describe a number of unmet needs. As a result, a number of interventions have been designed to support carers, including the “Maudsley eating disorder collaborative care skills workshops,” which comprise six 2‐hr workshops delivered over 3 months for parents and carers of people with EDs. The current study aimed to test a proof‐of‐concept that this workshop could be effectively delivered in 1 day. An additional aim was to assess whether the workshop had direct effects on carer skills. A nonexperimental repeated measures research design was employed, giving measures before and after a 1‐day workshop. Results suggested significant increases in carer self‐efficacy and carer skills, with moderate to large effect sizes. Qualitative analyses supported these results whilst also generating ideas to improve the 1‐day workshop.
Performance and Ageing Robustness of Graphite/NMC Pouch Prototypes Manufactured through Eco‐Friendly Materials and Processes
Authors: Loeffler Nicholas , Kim Guk.‐T. , Passerini Stefano , Gutierrez Cesar , Cendoya Iosu , De Meatza Iratxe , Alessandrini Fabrizio , Appetecchi Giovanni B. 
Published: August 2017

AbstractGraphite/lithium nickel‐manganese‐cobalt oxide (NMC), stacked pouch cells with nominal capacity of 15–18 Ah were designed, developed, and manufactured for automotive applications in the frame of the European Project GREENLION. A natural, water‐soluble material was used as the main electrode binder, thus allowing the employment of H2O as the only processing solvent. The electrode formulations were developed, optimized, and upscaled for cell manufacturing. Prolonged cycling and ageing tests revealed excellent capacity retention and robustness toward degradation phenomena. For instance, above 99 % of the initial capacity is retained upon 500 full charge/discharge cycles, corresponding to a fading of 0.004 % per cycle, and about 80 % of the initial capacity is delivered after 8 months ageing at 45 °C. The stacked soft‐packaged cells have shown very reproducible characteristics and performance, reflecting the goodness of design and manufacturing.