bluuebunny/crossref_metadata_2025_split huggingface dataset:
Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles
Authors: Abalde-Cela Sara , Taladriz-Blanco Patricia , de Oliveira Marcelo Ganzarolli , Abell Chris 
Published: January 2018

AbstractThe synthesis of anisotropic metallic nanoparticles (NPs) has been a field of intense and challenging research in the past decade. In this communication, we report on the reproducible and highly controllable synthesis of monodisperse branched gold nanoparticles in a droplet-based microfluidics platform. The process has been automated by adapting two different bulk synthetic strategies to microdroplets, acting as microreactors, for NP synthesis: a surfactant-free synthesis and a surfactant-assisted synthesis. Microdroplets were generated in two different microfluidic devices designed to accommodate the requirements of both bulk syntheses. The epitaxial growth of AuNSTs inside the microdroplets allowed for a fine control of reagent mixing and local concentrations during particle formation. This is the first time branched gold NPs have been synthesised in a microfluidics platform. The monodispersity of the product was comparable to the synthesis in bulk, proving the potential of this technology for the continuous synthesis of high quality anisotropic NPs with improved reproducibility.
Slow Wave Applications of Electromagnetically Induced Transparency in Microstrip Resonator
Authors: Amin Muhammad , Ramzan Rashad , Siddiqui Omar 
Published: January 2018

AbstractWe report a novel guided-wave resonator that supports multiple bands of electromagnetically induced transparency (EIT). The platform for the spatial and spectral interference is obtained by a microstrip transmission line loaded with proximity-coupled open-circuited stubs. We show experimentally that with two microstrip open stubs, a complete destructive interference takes place leading to a single EIT band with near-unity transmission efficiency. More interestingly, the addition of a third stub results in a supplementary EIT band with a Q-factor of 147 and an effective group refractive index of 530. With the open-stub configuration, the EIT phase response can be dynamically controlled by varying the capacitance between the adjacent stubs without breaking the transmission path of the underlying electromagnetic waves. Therefore, the proposed structure is well suited for buffering and tunable phase modulation applications. Since the proposed structures are compact and fully planar, we anticipate seamless integration with low-profile high frequency electronics.
Ubiquinone binding site of yeast NADH dehydrogenase revealed by structures binding novel competitive- and mixed-type inhibitors
Authors: Yamashita Tetsuo , Inaoka Daniel Ken , Shiba Tomoo , Oohashi Takumi , Iwata So , Yagi Takao , Kosaka Hiroaki , Miyoshi Hideto , Harada Shigeharu , Kita Kiyoshi , Hirano Katsuya 
Published: January 2018

AbstractYeast Ndi1 is a monotopic alternative NADH dehydrogenase. Its crystal structure in complex with the electron acceptor, ubiquinone, has been determined. However, there has been controversy regarding the ubiquinone binding site. To address these points, we identified the first competitive inhibitor of Ndi1, stigmatellin, along with new mixed-type inhibitors, AC0-12 and myxothiazol, and thereby determined the crystal structures of Ndi1 in complexes with the inhibitors. Two separate binding sites of stigmatellin, STG-1 and STG-2, were observed. The electron density at STG-1, located at the vicinity of the FAD cofactor, further demonstrated two binding modes: STG-1a and STG-1b. AC0-12 and myxothiazol are also located at the vicinity of FAD. The comparison of the binding modes among stigmatellin at STG-1, AC0-12, and myxothiazol revealed a unique position for the aliphatic tail of stigmatellin at STG-1a. Mutations of amino acid residues that interact with this aliphatic tail at STG-1a reduced the affinity of Ndi1 for ubiquinone. In conclusion, the position of the aliphatic tail of stigmatellin at STG-1a provides a structural basis for its competitive inhibition of Ndi1. The inherent binding site of ubiquinone is suggested to overlap with STG-1a that is distinct from the binding site for NADH.
Carbon fibers coated with graphene reinforced TiAl alloy composite with high strength and toughness
Authors: Cui Sen , Cui Chunxiang , Xie Jiaqi , Liu Shuangjin , Shi Jiejie 
Published: January 2018

AbstractTo meet the more rigorous requirement in aerospace industry, recent studies on strengthening and toughening TiAl alloys mostly focus on high Nb addition, which inevitably bring in an increasing of density. In this study, a carbon fibers coated with graphene reinforced TiAl alloy composite was fabricated by powder metallurgy, melt spun and vacuum melting. This composite got remarkable mechanical properties combined with a prominent density reduction. In contrast with pure TiAl ingots, this sample exhibits an average fracture strain from 16% up to 26.27%, and an average strength from 1801 MPa up to 2312 MPa. Thus, we can achieve a new method to fabricate this low-density, good mechanical performance TiAl composite which could bring in more opportunities for application in aerospace industry.
A new strategy for the passive skin delivery of nanoparticulate, high molecular weight hyaluronic acid prepared by a polyion complex method
Authors: Tokudome Yoshihiro , Komi Tatsuya , Omata Ami , Sekita Marie 
Published: January 2018

AbstractRestoring hyaluronic acid (HA) content is important for maintaining the function of photo-aged skin. This study aimed to evaluate the passive delivery into skin of HA nanoparticles formed by the polyion complex method. Nanoparticles were prepared by mixing and stirring anionic HA with a cationic polymer, protamine, at the charge ratio 55:45. The permeation of fluorescently-labelled HA nanoparticles (HANP) or free HA through hairless mouse skin was characterized in vitro. HANP or free HA was applied to ultraviolet (UV)-irradiated mice in vivo, and their transepidermal water loss (TEWL) was measured after 4 days. HA that had been delivered into skin was separated and characterized by molecular sieve chromatography. HANP were able to deliver HA into the dermis both in vitro and in vivo, whereas free HA penetrated no further than the stratum corneum. Following HANP application, HA within the skin was present in the form of free HA rather than nanoparticles. When applied in vivo, HANP significantly reduced the TEWL caused by UV irradiation. Thus, although free HA does not penetrate into the skin by passive diffusion, HA can be effectively delivered by nanoparticles. HA is then released from the nanoparticles and can contribute to barrier recovery following UV irradiation.
Generation of a rod-specific NRL reporter line in human pluripotent stem cells
Authors: Phillips M. Joseph , Capowski Elizabeth E. , Petersen Andrew , Jansen Alex D. , Barlow Katherine , Edwards Kimberly L. , Gamm David M. 
Published: January 2018

AbstractReporter lines generated in human pluripotent stem cells can be highly useful for the analysis of specific cell types and lineages in live cultures. We created the first human rod reporter line using CRISPR/Cas9 genome editing to replace one allele of the Neural Retina Leucine zipper (NRL) gene with an eGFP transgene in the WA09 human embryonic stem cell (hESC) line. After confirming successful targeting, three-dimensional optic vesicle structures were produced to examine reporter specificity and to track rod differentiation in culture. The NRL+/eGFP hESC line robustly and exclusively labeled the entirety of rods throughout differentiation, eventually revealing highly mature structural features. This line provides a valuable tool for studying human rod development and disease and testing therapeutic strategies for retinitis pigmentosa.
Assessment of future climate change impacts on nonpoint source pollution in snowmelt period for a cold area using SWAT
Authors: Wang Yu , Bian Jianmin , Zhao Yongsheng , Tang Jie , Jia Zhuo 
Published: January 2018

AbstractThe source area of Liao River is a typical cold region in northeastern China, which experiences serious problems with agricultural nonpoint source pollution (NPS), it is important to understand future climate change impacts on NPS in the watershed. This issue has been investigated by coupling semi distributed hydrological model (SWAT), statistical downscaling model (SDSM) and global circulation model (GCMs). The results show that annual average temperature would rise by 2.1 °C (1.3 °C) in the 2080 s under scenario RCP8.5 (RCP4.5), and annual precipitation would increase by 67 mm (33 mm). The change in winter temperature and precipitation is most significant with an increase by 0.23 °C/10a (0.17 °C/10a) and 1.94 mm/10a (2.78 mm/10a). The future streamflow, TN and TP loads would decrease by 19.05% (10.59%), 12.27% (8.81%) and 10.63% (6.11%), respectively. Monthly average streamflow, TN and TP loads would decrease from March to November, and increase from December to February. This is because the increased precipitation and temperature in winter, which made the spring snowpack melting earlier. These study indicate the trends of nonpoint source pollution during the snowmelt period under climate change conditions, accordingly adaptation measures will be necessary.
Tripartite symbiosis of plant-weevil-bacteria is a widespread phenomenon in the Negev Desert
Authors: Bar-Shmuel Nitsan , Rogovin Elena , Rachmilevitch Shimon , Friedman Ariel-Leib-Leonid , Shelef Oren , Hoffmann Ishai , Rosenberg Tamir , Behar Adi , Shavit Reut , Meng Fengqun , Segoli Michal 
Published: January 2018

AbstractThe weevilConorhynchus palumbusdevelops in a mud chamber affixed to the roots of the summer annual plantSalsola inermisin the Negev Desert of Israel. The weevil carries nitrogen fixing bacteria, and evidence suggests that plants with weevils utilize the fixed nitrogen. To characterize the distribution, abundance and significance of this unique interaction, we surveyedSalsolaplants in 16 sites throughout the Negev Desert. We excavated ~100 plants from each site, recorded the presence of weevils in their roots, and characterized the soil properties in each site. Weevil mud chambers were present in all of the sampled sites and their abundance was positively correlated with soil nitrogen content and with plant size, and negatively correlated with soil grain-size. Intriguingly, we found two additional weevil species–Menecleonus virgatusandMaximus mimosae–residing in mud chambers onSalsolaroots, and found one additionalSalsolaspecies–S. incanescens–accommodating weevils. Nitrogen fixing bacteria were found in weevil larvae of the two additional species and at multiple sites. Overall, our findings suggest that potentially beneficial associations between weevils and plants may be more common than previously acknowledged, and may play an important role in this desert ecosystem.
Identification and caste-dependent expression patterns of DNA methylation associated genes in Bombus terrestris
Authors: Li Beibei , Hou Li , Zhu Dan , Xu Xilian , An Shiheng , Wang Xianhui 
Published: January 2018

AbstractDNA methylation has been proposed to play critical roles in caste fate and behavioral plasticity in bumblebees, however, there is little information on its regulatory mechanisms. Here, we identified six important genes mediating the modification of DNA methylation and determined their expression patterns in the bumblebee Bombus terrestris. There is a complete functional DNA methylation system, including four DNA methyltransferases (DNMT1a, DNMT1b, DNMT2, and DNMT3), a DNA demethylase (Ten-eleven translocation), and a methyl-CpG-binding domain protein in B. terrestris. Most of these genes were highly expressed in fat bodies and gonads but lowly expressed in antennae and brains of bumblebee adults. Besides, these genes exhibited caste-specific expression patterns in bumblebees, with higher transcription levels in queens than workers and drones. Whereas their expression levels showed no remarkable difference in queenright and queenless workers. These results suggested potential roles of DNA methylation-related genes in caste differentiation in bumblebees.
Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson’s disease model
Authors: Stephano Flora , Nolte Stella , Hoffmann Julia , El-Kholy Samar , von Frieling Jakob , Bruchhaus Iris , Fink Christine , Roeder Thomas 
Published: January 2018

AbstractParkinson’s disease, which is the one of the most common neurodegenerative movement disorder, is characterized by a progressive loss of dopamine containing neurons. The mechanisms underlying disease initiation and development are not well understood and causative therapies are currently not available. To elucidate the molecular processes during early stages of Parkinson’s disease, we utilized a Drosophila model. To induce Parkinson’s disease-like phenotypes, we treated flies with the pesticide rotenone and isolated dopamine producing neurons of animals that were at an early disease stage. Transcriptomic analyses revealed that gene ontologies associated with regulation of cell death and neuronal functions were significantly enriched. Moreover, the activities of the MAPK/EGFR- and TGF-β signaling pathways were enhanced, while the Wnt pathway was dampened. In order to evaluate the role of Wnt signaling for survival of dopaminergic neurons in the disease model, we rescued the reduced Wnt signaling activity by ectopic overexpression of armadillo/β-catenin. This intervention rescued the rotenone induced movement impairments in the Drosophila model. Taken together, this initial study showed a highly relevant role of Wnt signaling for dopamine producing neurons during pathogenesis in Parkinson’s disease and it implies that interfering with this pathway might by a suitable therapeutic option for the future.
p38 activation induces production of miR-146a and miR-31 to repress E-selectin expression and inhibit transendothelial migration of colon cancer cells
Authors: Zhong Liang , Huot Jacques , Simard Martin J. 
Published: January 2018

AbstractExtravasation of circulating cancer cells determines their metastatic potential. This process is initiated by the adhesion of cancer cells to vascular endothelial cells through specific interactions between endothelial adhesion receptors such as E-selectin and their ligands on cancer cells. In the present study, we show that miR-146a and miR-181b impede the expression of E-selectin by repressing the activity of its transcription factor NF-κB, thereby impairing the metastatic potentials of colon cancer cells by decreasing their adhesion to, and migration through, the endothelium. Among the two microRNAs, only miR-146a is activated by IL-1β, through the activation of p38, ERK and JNK MAP kinases, as well as their downstream transcription factors GATA2, c-Fos and c-Jun. Inhibiting p38 MAP kinase increases NF-κB activity, at least partially via miR-146a. Inhibiting p38 also increases the expression of E-selectin at the post-transcriptional level via decreasing miR-31, which targets E-selectin mRNA and also depends on p38 for its expression. In response to IL-1β, p38 MAP kinase hence represses the expression of E-selectin at the transcriptional and the post-transcriptional levels, via miR-146a and miR-31, respectively. These results highlight novel mechanisms by which p38 downregulates the expression of E-selectin through different microRNAs following inflammatory stimuli associated to cancer progression.
Characterizations of distinct parallel and antiparallel G-quadruplexes formed by two-repeat ALS and FTD related GGGGCC sequence
Authors: Zhou Bo , Geng Yanyan , Liu Changdong , Miao Haitao , Ren Yaguang , Xu Naining , Shi Xiao , You Yingying , Lee Tunglun , Zhu Guang 
Published: January 2018

AbstractThe large expansion of GGGGCC (G4C2) repeats of the C9orf72 gene have been found to lead to the pathogenesis of devastating neurological diseases, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The structural polymorphisms of C9orf72 HRE DNA and RNA may cause aberrant transcription and contribute to the development of ALS and FTD. Here we showed that the two-repeat G4C2 DNA, d(G4C2)2, simultaneously formed parallel and antiparallel G-quadruplex conformations in the potassium solution. We separated different folds of d(G4C2)2 by anion exchange chromatography, followed with characterizations by circular dichroism and nuclear magnetic resonance spectroscopy. The parallel d(G4C2)2 G-quadruplex folded as a symmetric tetramer, while the antiparallel d(G4C2)2 adopted the topology of an asymmetric dimer. These folds are distinct from the antiparallel chair-type conformation we previously identified for the d(G4C2)4 G-quadruplex. Our findings have demonstrated the conformational heterogeneity of the C9orf72 HRE DNA, and provided new insights into the d(G4C2)n folding. Meanwhile, the purified d(G4C2)2 G-quadruplex samples are suitable for further three-dimensional structure characterizations, which are required for the structure-based design of small molecules targeting ALS and FTD related C9orf72 HRE.
Variation in Raw Milk Microbiota Throughout 12 Months and the Impact of Weather Conditions
Authors: Li Nan , Wang Yuezhu , You Chunping , Ren Jing , Chen Wanyi , Zheng Huajun , Liu Zhenmin 
Published: January 2018

AbstractMilk microbiota has a great influence on the safety and quality of dairy products. However, few studies have investigated the variations of bacterial composition in raw milk. In this study, raw milk samples were collected in 12 successive months, and their bacterial compositions were determined by 16 S rRNA gene sequencing. The highest diversity of bacterial composition was detected in June, while the lowest was in December.Firmicutes,ProteobacteriaandActinobacteriawere the most abundant phyla and exhibited a counter-balanced relationship.Pseudomonas,LactococcusandAcinetobacterwere the most prevalent genera (>1%), and a tiny core microbiota (AcinetobacterandPseudomonas) was observed. Temperature and humidity were the determining factors for most variation in bacterial compositions at both the phylum and genus levels. Higher abundances ofPseudomonas,PropionibacteriumandFlavobacteriumwere correlated with low temperature. Furthermore,Pseudomonas/PropionibacteriumandLactobacillus/Bifidobacteriumwere two pairs of genera that had synergistic effects. Associations between the microbiota and milk quality parameters were analyzed. The abundances ofPropionibacterium and Pseudoalteromonaswere negatively correlated to total bacterial count, which meant that they helped to maintain milk quality, while a series of environmental microorganisms contributed to the spoilage of raw milk.
Modeling the Intervention of HIV Transmission across Intertwined Key Populations
Authors: Zhong Lu , Zhang Qingpeng , Li Xiaoming 
Published: January 2018

AbstractThe HIV transmissions between multiple key populations make interventions difficult, particularly with multiple transmission behaviors. It remains unclear how significant the role of bridge individuals (who connect multiple communities) is in HIV transmission, and how to develop more effective intervention strategies targeting different transmission modes across key populations. In this research, we proposed a 2-layer social network framework to simulate the HIV transmissions across female sex workers (FSWs) and persons who inject drugs (PWID) through two behaviors: unprotected sex and needle-sharing. We proposed a set of intervention strategies based on the topological properties of individuals in the social network and estimated the efficacy of these strategies. Simulation studies demonstrated that bridge individuals played a significant role in HIV transmissions across the two networks. Prevention on such bridge individuals could help reduce both the scale and speed of HIV transmissions.
Development of chipless, wireless current sensor system based on giant magnetoimpedance magnetic sensor and surface acoustic wave transponder
Authors: Kondalkar Vijay V. , Li Xiang , Park Ikmo , Yang Sang Sik , Lee Keekeun 
Published: January 2018

AbstractA chipless, wireless current sensor system was developed using a giant magnetoimpedance (GMI) magnetic sensor and one-port surface acoustic wave (SAW) reflective delay line for real-time power monitoring in a current-carrying conductor. The GMI sensor has a high-quality crystalline structure in each layer, which contributes to a high sensitivity and good linearity in a magnetic field of 3–16 Oe. A 400 MHz RF energy generated from the interdigital transducer (IDT)-type reflector on the one-port SAW delay line was used as an activation source for the GMI magnetic sensor. The one-port SAW delay line replaces the presently existing transceiver system, which is composed of thousands of transistors, thus enabling chipless and wireless operation. We confirmed a large variation in the amplitude of the SAW reflection peak with a change in the impedance of the GMI sensor caused by the current flow through the conductor. Good linearity and sensitivity of ~0.691 dB/A were observed for currents in the range 1–12 A. Coupling of Mode (COM) modeling and impedance matching analysis were also performed to predict the device performance in advance and these were compared with the experimental results.
Histone H5 is a potent Antimicrobial Agent and a template for novel Antimicrobial Peptides
Authors: Jodoin Joelle , Hincke Maxwell T. 
Published: January 2018

AbstractModern medicine is challenged continuously by the increasing prevalence of antibiotic resistant bacteria. Cationic antimicrobial peptides and their derivatives are interesting potential alternatives to antibiotics due to their rapid action, broad-spectrum of antimicrobial activity and limited emergence of bacterial resistance. This study reports the novel antimicrobial properties of histone H5, purified from chicken erythrocytes, and histone H5-derived synthetic peptides. Broth microdilution assays revealed that histone H5 has potent broad-spectrum antimicrobial activity against Gram-positive and Gram-negative planktonic bacteria (MIC range: 1.9 ± 1.8 to 4.9 ± 1.5 µg/mL), including vancomycin-resistant Enterococcus (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Moreover, histone H5 displayed anti-biofilm activity against established Listeria monocytogenes and Pseudomonas aeruginosa biofilms. Scanning electron microscopy demonstrated bacterial membrane damage after histone H5 treatment, while a hemolytic assay revealed that histone H5 is non-toxic towards mammalian erythrocytes, even at a concentration of 1 mg/mL. Although the predicted H5-derived antimicrobial peptides tested in this study were located within the antimicrobial domain of histone H5, their synthetic versions did not possess more potent antimicrobial activity than the full length protein. Overall, this study demonstrates that histone H5 is a potent antimicrobial and therefore a promising template for the development of novel histone H5-derived antimicrobial peptides.