bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formal- and high-structured kinetic process modelling and footprint area analysis of binary imaged cells: Tools to understand and optimize multistage-continuous PHA biosynthesis
Authors: Koller Martin , Vadlja Denis , Braunegg Gerhart , Atlić Aid , Horvat Predrag 
Published: July 2017

Abstract
Competitive polyhydroxyalkanoate (PHAs) production requires progress in microbial strain performance, feedstock selection, downstream processing, and more importantly according to the process design with process kinetics of the microbial growth phase and the phase of product formation. The multistage continuous production in a bioreactor cascade was described for the first time in a continuously operated, flexible five-stage bioreactor cascade that mimics the characteristics involved in the engineering process of tubular plug flow reactors. This process was developed and used for Cupriavidus necator-mediated PHA production at high volumetric and specific PHA productivity (up to 2.31 g/(Lh) and 0.105 g/(gh), respectively). Based on the experimental data, formal kinetic and high structured kinetic models were established, accompanied by footprint area analysis of binary imaged cells. As a result of the study, there has been an enhanced understanding of the long-term continuous PHA production under balanced, transient, and nutrient-deficient conditions that was achieved on both the micro and the macro kinetic level. It can also be concluded that there were novel insights into the complex metabolic occurrences that developed during the multistage- continuous production of PHA as a secondary metabolite. This development was essential in paving the way for further process improvement. At the same time, a new method of specific growth rate and specific production rate based on footprint area analysis was established by using the electron microscope.
Antifungal activity of a biosurfactant-producing lactic acid bacteria strain
Authors: Matei Gabi Mirela , Matei Sorin , Matei Adrian , Draghici Elena 
Published: July 2017

Abstract
Lactic acid bacteria are frequently utilized in food industry and they are also recognized as antimicrobial agents due to their capability to produce metabolites such as: organic acids, biosurfactants, bacteriocins, hydrogen peroxide, cyclic dipeptides, exopolysaccharides. The main goal of this paper was to present the results of the research carried out on the strain LCM2 of lactic acid bacteria isolated from brined cucumbers, for production of biosurfactants and to assess its antifungal properties. The emulsification capacity of biosurfactant was measured using kerosene as the hydrophobic substrate. The value of emulsification index E24 was 89.04% showing a high emulsification activity of the biosurfactant. The structural characterization of biosurfactant by TLC revealed its glycolipidic nature. Assay of the ionic charge established the anionic charge of the biosurfactant revealed by the presence of precipitation lines towards the cationic surfactant dodecyl-dimethyl-ammonium chloride. The biosurfactant presented antibiofilm activity with low adherence capacity, structural damages of the hyphal net, conidiophores and delays or lack of sporulation and decreased biomass accumulation in four mycotoxigenic Penicillium and Aspergillus isolates. Results of in vitro assays recommend the biosurfactant produced by the new lactic acid bacteria strain LCM2 for biotechnological purposes, as alternative antifungal agent in food industry.
Synthesis of disulphide-Schiff base derivatives and investigations of in vitro antimicrobial activities against some human pathogens
Authors: Durmuş Sefa , Dalmaz Aslıhan , Dülger Görkem , Kadıoğlu Duygu Bircan 
Published: July 2017

Abstract
Thio-Schiff bases are becoming increasingly widespread in various branches such as the preparation of certain medicines, cosmetic products, and polymer production. In particular, the presence of antibacterial, antifungal, antiviral, antitumor and antimalarial properties of Schiff bases containing sulfur in the structure has made these compounds attractive in different disciplines. In this study, different derivatives of dimeric disulfide-Schiff bases have been synthesized. The antibacterial and antifungal activities of the synthesized these compounds were investigated in vitro against some human pathogens (Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Candida albicans, C. tropicalis, C. guilliermondii and C. glabrata). Test microorganisms were isolated from the patients appyling to Medical Faculty Hospital of Duzce University were used. Diffusion method was used to determine the antimicrobial activities of the compounds.standard antibacterial (Cefotaxime, Amoxicillin/clavulanicacid) and antifungal (Posaconazole) antibiotics were used as the control group and the results were compared. The result indicated that antimicrobial activity of Disulphide-Schiff Base Derivatives exhibited less activity against bacteria as compared to AMC30 (Amoxicillin/clavulanicacid), but highly effective against bacteria as compared to CTX30 (Cefotaxime). In addition, the compounds exhibited less activity against yeast.
Synthesis and analgesic activities of some new 5-chloro-2(3H)-benzoxazolone derivatives
Authors: Mulazim Yusuf , Berber Cevdet , Erdogan Hakkı , Ozkan Melike Hacer , Kesanli Banu 
Published: July 2017

Abstract
Affordable and practical synthesis methods in drug development have always been very attractive. Herein, microwave assisted synthesis was utilized to prepare piperazine substituted 5-chloro-2(3H)-benzoxazolone derivatives in 5 minutes. Structural characterization of these 5-chloro-2(3H)-benzoxazolone derivatives was achieved by IR, NMR, ESI-MS and elemental analysis. Since these types of compounds have been shown to have anti-inflammatory and analgesic activities there biological activities were also examined. Indomethacin (INDO) and acetylsalicylic acid (ASA) were used as reference. Carrageenan-induced hind paw edema in mice test was used to study anti-inflammatory activity. Compound 1 (100 mg / kg dose) showed the longest anti-inflammatory activity among the title compounds synthesized. For the analgesic activities, both hot-plate and tail-flick tests were employed. Compound 3 was found to have the highest activity in the hot-plate test whereas in the tail-flick test, compounds 1 and 2 showed higher anti-nociceptive activity.
Comparative analysis of drought responses in Phaseolus vulgaris (common bean) and P. coccineus (runner bean) cultivars
Authors: Morosan Mihaela , Hassan Mohamad Al , Naranjo Miguel A. , López-Gresa María P. , Boscaiu Monica , Vicente Oscar 
Published: July 2017

Abstract
Genetic improvement of crop drought tolerance has become an urgent need for increasing agricultural yields and food production, to feed a growing human population in the context of global climate change. To get insights into the most relevant mechanisms underlying drought resistance in beans, we have analysed the responses to water deficit of three Phaseolus vulgaris (common bean) and one P. coccineus (runner bean) cultivars, focusing on the accumulation of specific osmolytes, a conserved response to abiotic stress in plants. Changes in osmolyte levels were correlated with the relative tolerance to water stress of the studied cultivars. Drought tolerance in Phaseolus largely depends on the accumulation of myo-inositol; glycine betaine may also contribute to tolerance in P. coccineus (but not in P. vulgaris). Proline, another common osmolyte, is a reliable marker of the level of stress affecting bean plants, but is not directly involved in tolerance mechanisms, as its drought-dependent accumulation is lowest in the most tolerant cultivar. We suggest that, by measuring the levels of proline and myo-inositol in water-stressed plants, a large number of cultivars could be easily and rapidly screened to select promising candidates to be used in breeding programmes for improving drought tolerance in beans.
THE CUSTOMER LOYALTY OF PATAS PURWAKARTA TRAIN SERVICE USERS
Authors: Riyaldi Muhamad Sapta , Amrizal Amrizal , Silalahi Tiur Merry Bunga 
Published: July 2017

The aim of this research is to find out the influence of service quality, ticket price, and customer satisfaction to the customer loyalty. It is a quantitative research using the data analysis method of Path Analysis. The results indicate that both variables of Service Quality (X1) and Ticket Price (X2) have a significant direct influence to the variable of Customer Satisfaction (Y1), whereas the variable of customer satisfaction has a significant indirect influence to the variable of Customer Loyalty (Y2) [through intervening the variable of customer satisfaction]. It is found in a sequence that the variables most influential to the increase of Customer Loyalty are: Service Quality, Customer Satisfaction, and the Ticket Price in the last rank.The results of path analysis indicate that service quality can directly influence the customer loyalty. It can be optimistically improved as maximal as possible through 5 factors having positive influences in enhancing customer loyalty. Meanwhile, Ticket Price still indirectly influences the customer loyalty and it can pesimistically still be enhanced through the policy on tariff and it is difficult to implement, only can be driven if the ticket price provided by the transport service provider is more competitive and more various as well as affordable for the consumer purchase power of that transport sevice.
THE EFFECT OF RECRUITMENT AND EMPLOYEE SELECTION ON EMPLOYEE PLACEMENT AND ITS IMPACTS TOWARDS EMPLOYEE PERFORMANCE AT PT SRIWIJAYA AIR
Authors: Sarinah Sarinah , Gultom Rahmat Simon , Thabah Asep Ali 
Published: July 2017

Sriwijaya Air realizes the importance of candidates selection of process, because this process will result in employees in accordance with the prescribed qualifying so as to achieve a performance from these new employees. The purpose of this study was to determine the effect of the implementation of the recruitment and placement of employees selection and its impact on the performance of employees of PT Sriwijaya Air Jakarta. The analytical method used is the method of path analysis. The results showed that recruitment and selection variables influence significantly on the placement of employees. Likewise, employee placement variable influence significantly on the performance of employees of PT Sriwijaya Air Jakarta. However, only recruitment variable that Significantly influence on employee performance. Recruitment is one - the only variable that has direct influence on employee performance. While selection and placement of employees variable do not affect the performance of employees.
IntroMap: A Pipeline and Set of Diagnostic DiploidArachisSNPs as a Tool for Mapping Alien Introgressions inArachis hypogaea
Authors: Clevenger J. , Bertioli D.J. , Leal-Bertioli S.C.M. , Chu Y. , Stalker H.T. , Ozias-Akins P. 
Published: July 2017

ABSTRACTFor crops with a narrow cultivated genetic base, incorporating beneficial alleles from related species through alien introgression widens the genetic base and provides key resistances to disease and abiotic stresses. Fine mapping of these introgressions can increase the efficiency of marker-assisted selection for breeding programs. To facilitate high resolution fine mapping of alien introgressions, we developed an automated pipeline, IntroMap. This pipeline was developed with accessibility and utility in mind, and does not present novel mapping algorithms. Using five diploid wild Arachis species, we identified diagnostic SNP sets for introgression mapping in Arachis hypogaea, cultivated peanut. IntroMap has applicability in all crops where alien introgression is used to bring in beneficial alleles from related species, so the pipeline includes an option to generate new diagnostic SNPs from any species/accession of interest for use in the pipeline. These user generated resources will be included for distribution with IntroMap to increase the SNP resources for all users. We demonstrate the efficacy of IntroMap by fine mapping three alien introgressions in an elite peanut breeding line with superior disease resistance. IntroMap works well even at low coverage, recovering at 2x coverage almost 50% of the diagnostic SNPs found at 10x coverage. The true benefit of IntroMap is the availability and generation of shared public resources, specifically for Arachis spp. IntroMap is freely distributed at https://sourceforge.net/projects/intromap/.
Monitoring Fungicide Sensitivity Levels and Mycelial Compatibility Groupings of Sclerotium rolfsii Isolates from Florida Peanut Fields
Authors: Khatri K. , Kunwar S. , Barocco R. L. , Dufault N. S. 
Published: July 2017

ABSTRACT
Sclerotium rolfsii, the causal agent of peanut stem rot, is a diverse pathogen that has exhibited decreases in sensitivity to fungicides in areas where they are frequently applied. To better understand this pathogen's diversity and its response to various fungicides in Florida, a monitoring survey was done to examine isolates from several peanut producing areas using morphological characteristics, mycelial compatibility groupings and fungicide sensitivity profiles. A high level of morphological diversity was observed among a small number (N = 15) of isolates which was affirmed by both Shannon-Weiner (E = 0.812) and Simpson's (D = 0.280) indices. However, despite this high level of diversity, fungicide sensitivity of these isolates to flutolanil (EC50 = 0.031 ppm) and tebuconazole (EC50 = 0.008 ppm) appears to remain relatively unchanged when compared to a previous baseline study. Utilizing a small number of isolates, this monitoring survey indicated the EC50 values for the products azoxystrobin (EC50 = 0.050 ppm), prothioconazole (EC50 = 0.213 ppm), penthiopyrad (EC50 = 0.016 ppm) and solatenol (EC50 = 0.005 ppm). A trend for hormesis was also noted in this survey (e.g. flutolanil), but further research is necessary to better understand sub-lethal fungicide dose effects on increasing mycelial growth. It is apparent from these results that despite the high levels of phenotypic diversity in S. rolfsii populations, current fungicide management practices should remain effective for disease control.
Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2
Authors: Saravanan Renuka , Pemaiah Brindha , Sridharan Sriram , Narayanan Mahesh , Ramalingam Sivakumar 
Published: July 2017

The aim of the present study was to analyze the anti-cancer effect of Orthosiphon stamineus leaf extract on MCF-7 cells. Ethyl acetate extract of the plant was eluted and pooled into 6 fractions based on their Rf values and the fractions were tested for cytotoxicity. Fraction 3 was found effective (IC50 = 28.5 µL/mL) and was subjected to LC-MS analysis which indicated the presence of biochanin, eleutherol and cinnamic acid. Further, cytotoxic effect of the fraction was confirmed in terms of nucleolin, bcl2, bax and cytochrome c expression in MCF-7 cells through RT-PCR where a marked decrease in nucleolin, bcl2 and increase in bax and cytochrome c were observed. The presence of eleutherol, a well-known anti-oxidant and anti-cancer agent, has been reported for the first time in O. stamineus. The anti-cancer activity of O. stamineus may be due to the presence of elutherol and cinnamic acid.
Video Clip of Methodology:
LC-MS analysis of Orthosiphon stamineus extract: 7 min 33 sec Full Screen Alternate
Carbon Chain Anions and the Growth of Complex Organic Molecules in Titan’s Ionosphere
Authors: Desai R. T. , Coates A. J. , Wellbrock A. , Vuitton V. , Crary F. J. , González-Caniulef D. , Shebanits O. , Jones G. H. , Lewis G. R. , Waite J. H. , Cordiner M. , Taylor S. A. , Kataria D. O. , Wahlund J.-E. , Edberg N. J. T. , Sittler E. C. 
Published: July 2017

Abstract

Cassini discovered a plethora of neutral and ionized molecules in Titan’s ionosphere including, surprisingly, anions and negatively charged molecules extending up to 13,800 u q−1. In this Letter, we forward model the Cassini electron spectrometer response function to this unexpected ionospheric component to achieve an increased mass resolving capability for negatively charged species observed at Titan altitudes of 950–1300 km. We report on detections consistently centered between 25.8 and 26.0 u q−1 and between 49.0–50.1 u q−1 which are identified as belonging to the carbon chain anions, CN−/C3N− and/or C2H−/C4H−, in agreement with chemical model predictions. At higher ionospheric altitudes, detections at 73–74 u q−1 could be attributed to the further carbon chain anions C5N−/C6H− but at lower altitudes and during further encounters extend over a higher mass/charge range. This, as well as further intermediary anions detected at >100 u, provide the first evidence for efficient anion chemistry in space involving structures other than linear chains. Furthermore, at altitudes below <1100 km, the low-mass anions (<150 u q−1) were found to deplete at a rate proportional to the growth of the larger molecules, a correlation that indicates the anions are tightly coupled to the growth process. This study adds Titan to an increasing list of astrophysical environments where chain anions have been observed and shows that anion chemistry plays a role in the formation of complex organics within a planetary atmosphere as well as in the interstellar medium.
Fauna Europaea: Hymenoptera – Symphyta &amp; Ichneumonoidea
Authors: van Achterberg Kees , Taeger Andreas , Blank Stephan , Zwakhals Kees , Viitasaari Matti , Yu Dicky , de Jong Yde 
Published: July 2017

Fauna Europaea provides a public web-service with an index of scientific names (including important synonyms) of all extant European terrestrial and freshwater animals, their geographical distribution at the level of countries and major islands (west of the Urals and excluding the Caucasus region), and some additional information. The Fauna Europaea project comprises about 230,000 taxonomic names, including 130,000 accepted species and 14,000 accepted subspecies, which is much more than the originally projected number of 100,000 species. Fauna Europaea represents a huge effort by more than 400 contributing specialists throughout Europe and is a unique (standard) reference suitable for many users in science, government, industry, nature conservation and education.
For the Hymenoptera, taxonomic data from one grade (Symphyta) and one Superfamily (Ichneumonoidea), including 15 families and 10,717 species, are included.
Ichneumonoidea is the largest superfamily of Hymenoptera and consisting of two extant families, Ichneumonidae and Braconidae. The costal cell of the fore wing is absent, the fore wing has at least two closed cells, the constriction between the mesosoma (thorax + first abdominal segment or propodeum) and the metasoma (remainder of abdomen) is distinct and the parasitoid larvae usually spin a silken cocoon. Also, the metasoma is ventrally partly desclerotized in the vast majority of ichneumonoids.
Mapping the dependency of crops on pollinators in Belgium
Authors: Jacquemin Floriane , Violle Cyrille , Rasmont Pierre , Dufrêne Marc 
Published: July 2017

Because of their pollinating activity, insect pollinators provide an ecosystem service that is essential to ecosystems and our economy. A large majority of the flowering plants we consume depends on it. In turn, the decline in pollinators observed for the last decades in Belgium as in many other European countries threatens agriculture and human well-being.
Here we evaluate the pollination service at a country-wide scale through the estimated value of the contribution of insect pollination to the production used for human consumption in Belgium using crop dependency ratios. We then mapped the vulnerability of crops in the face of pollinator decline at the provincial level.
We show that the part of plant production for human food that we can attribute to the action of insect pollinators represents a value of about 251.6 million euros in 2010 in Belgium. As a result, 11.1 % of total value of Belgian plant production (in terms of fruit quantity and quality) depend on pollinators.
Although the applied method to assess the pollination service estimates only a minimum value of a single aspect of the pollination service, it allows to target the areas of the country where this service is particularly at risk and where it is necessary to define pollinator conservation measures to maintain, and possibly restore current yields.
Big Data - Open Data : Quelles valeurs ? Quels enjeux ?
Authors: Turki Slim , Foulonneau Muriel , Turki Slim 
Published: July 2017

Malgré la masse des données disponibles, le nombre de services créés à partir des données ouvertes reste relativement marginal ; le nombre de services qui parviennent à la viabilité et la pérennité l’est encore plus. Les modèles économiques sous-jacents aux services basés sur les données ouvertes restent balbutiants. Cet article propose d’explorer l’écosystème sous-jacent à la publication et à la valorisation des données ouvertes et de dresser son réseau de valeurs. Il identifie les acteurs susceptibles de valoriser les données ouvertes, ainsi que les critères auxquels ils doivent être vigilants pour assurer la viabilité et la pérennité des services basés sur ces données.
Big Data - Open Data : Quelles valeurs ? Quels enjeux ?
Authors: Lrhoul Hanae , Chartron Ghislaine , Bachr Ahmed , Benammar Othman 
Published: July 2017

Cette étude vise à présenter une cartographie des publications de la Faculté de Médecine et de Pharmacie-Casablanca et de son évolution à travers le temps 1980-2013. Nous avons utilisé le logiciel Gephi, un outil de « datavisualisation » pour une meilleure représentation et analyse des indicateurs bibliométriques : évolution de la production médicale, les sources de publications des chercheurs et leurs réseaux de collaboration nationale et internationale. Dans la dernière partie, l’analyse des cartes des sciences offrira une vue globale sur la structure sociale de la recherche médicale (les communautés de chercheurs et leurs réseaux de collaboration)