bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphology of Brine‐Seawater Interface and Spatial Distribution of Submarine Groundwater Discharge Windows in the Muddy Coast
Authors: Zhang Yufeng , Sun Zengbing , Lei Bingxiao , Li Mingbo , Guo Xiujun , Zheng Tianyuan , Luo Jian 
Published: October 2024

AbstractThe brine‐seawater interface (BSI) is a unique type of groundwater‐seawater interface (GSI) characterized by the higher density of underground brine compared to seawater. This study focuses on characterizing the bay‐scale BSI morphology and identifying submarine‐groundwater discharge windows using a comprehensive in‐situ geophysical detection on the south bank of Laizhou Bay. Our findings reveal that the BSI forms an extensive mixing zone (15–20 km) without distinct contours between waters of varying salinities. The discharge windows for underground brine are located in nearshore areas with fine sand distribution and offshore pockmark areas. Hydraulic and salinity gradients drive the underground brine discharge through these windows. The aquitard window is the primary area for shallow and deep brine exchange, likely evolved from paleochannels, ancient tidal creeks, or ancient underwater barriers. These findings provide crucial modeling support for analyzing environmental evolution mechanisms and theoretical basis for planning the underground brine mining in similar coastal regions.
Land‐To‐Sea Mapping of the Glacial Erosion Unconformity Reveals Evolution of the Jasmund Glacitectonic Complex East of Rügen Island (SW Baltic Sea)
Authors: Haimerl B. , Seidel E. , Gehrmann A. , Preine J. , Schmidt M. C. , Hübscher C. 
Published: October 2024

AbstractGlacial movements shaped vast northern parts, offering critical insights into glacial dynamics in a changing climate. Located on the island of Rügen in NE Germany, the Jasmund Glacitectonic Complex (JGC) is a key area to study the dynamics of past glaciations. Previous reconstructions focused primarily on the onshore realm, resulting in some areas remaining unexplored. Here we use more than 140 high‐resolution marine multi‐channel seismic profiles to map the erosional unconformity surrounding the JGC for the first time. Submarine glacial features match features observed onshore, allowing a consistent land‐to‐sea reconstruction of the evolution of the JGC. Our results indicate a single SW‐directed Weichselian glacier advance, suggesting that the JGC formed through three distinct glacier lobes exerting pressure from multiple directions. The ice advance encircled the Jasmund peninsula and overthrusted Cretaceous sediments on the JGC perpendicularly and laterally.
Effects of Subauroral Polarization Streams on Ionospheric Radial Currents During the Geomagnetic Storm on 23 April 2023
Authors: Xia Hao , Wang Hui , Zhang Kedeng 
Published: October 2024

AbstractUsing observations from dual‐spacecraft (dual‐SC, Swarm A and C) and simulations from the Thermosphere‐Ionosphere Electrodynamics General Circulation Model (TIEGCM), this work investigates the ionospheric radial current (IRC) in response to subauroral polarization streams (SAPS) during the geomagnetic storm on 23 April 2023. At noon, a radially inward disturbance IRC (ΔIRC) emerges in response to SAPS, leading to a further intensification of inward IRC. The model simulation indicates that the dynamo current prevails over the polarization current, serving as the primary driver of the inward ΔIRC. Additionally, the significantly enhanced Pedersen conductivity potentially amplifies the inward ΔIRC. At dusk, ΔIRC exhibit a slight outward trend before 21 UT and a pronounced inward trend around 21–24 UT. This temporal variation is attributed to the equatorward propagation of SAPS‐induced thermospheric winds within 3–4 hr. The noontime inward electric field at the dip equator arises from both equatorward winds at low‐mid latitudes and local eastward winds. However, at dusk, the inward polarization electric field primarily stems from local disturbed eastward winds.
FGF4 and ascorbic acid enhance the maturation of induced cardiomyocytes by activating JAK2–STAT3 signaling
Authors: Jun Seongmin , Song Myeong-Hwa , Choi Seung-Cheol , Noh Ji-Min , Kim Kyung Seob , Park Jae Hyoung , Yoon Da Eun , Kim Kyoungmi , Kim Minseok , Hwang Sun Wook , Lim Do-Sun 
Published: October 2024

AbstractDirect cardiac reprogramming represents a novel therapeutic strategy to convert non-cardiac cells such as fibroblasts into cardiomyocytes (CMs). This process involves essential transcription factors, such as Mef2c, Gata4, Tbx5 (MGT), MESP1, and MYOCD (MGTMM). However, the small molecules responsible for inducing immature induced CMs (iCMs) and the signaling mechanisms driving their maturation remain elusive. Our study explored the effects of various small molecules on iCM induction and discovered that the combination of FGF4 and ascorbic acid (FA) enhances CM markers, exhibits organized sarcomere and T-tubule structures, and improves cardiac function. Transcriptome analysis emphasized the importance of ECM-integrin-focal adhesions and the upregulation of the JAK2–STAT3 and TGFB signaling pathways in FA-treated iCMs. Notably, JAK2–STAT3 knockdown affected TGFB signaling and the ECM and downregulated mature CM markers in FA-treated iCMs. Our findings underscore the critical role of the JAK2–STAT3 signaling pathway in activating TGFB signaling and ECM synthesis in directly reprogrammed CMs.
GPR56 facilitates hepatocellular carcinoma metastasis by promoting the TGF-β signaling pathway
Authors: Luo Yiming , Lu Junli , Lei Zhen , Rao Dean , Wang Tiantian , Fu Chenan , Zhu He , Zhang Zhiwei , Liao Zhibin , Liang Huifang , Huang Wenjie 
Published: October 2024

AbstractThe metastasis of hepatocellular carcinoma (HCC) poses a significant threat to the survival of patients. G protein-coupled receptor 56 (GPR56) has garnered extensive attention within malignant tumor research and plays a crucial role in cellular surface signal transmission. Nonetheless, its precise function in HCC remains ambiguous. Our investigation reveals a notable rise in GPR56 expression levels in human HCC cases, with heightened GPR56 levels correlating with unfavorable prognoses. GPR56 regulates TGF-β pathway by interacting with TGFBR1, thereby promoting HCC metastasis. At the same time, GPR56 is subject to regulation by the canonical cascade of TGF-β signaling, thereby establishing a positive feedback loop. Furthermore, the combination application of TGFBR1 inhibitor galunisertib (GAL) and GPR56 inhibitor Dihydromunduletone (DHM), significantly inhibits HCC metastasis. Interventions towards this signaling pathway could offer a promising therapeutic approach to effectively impede the metastasis of GPR56-mediated HCC.
Deciphering the impact of PROM1 alternative splicing on human photoreceptor development and maturation
Authors: Moya-Molina Marina , Dorgau Birthe , Flood Emily , Letteboer Stef J. F. , Lorentzen Esben , Coxhead Jonathan , Smith Graham , Roepman Ronald , Nagaraja Grellscheid Sushma , Armstrong Lyle , Lako Majlinda 
Published: October 2024

AbstractAlternative splicing (AS) is a crucial mechanism contributing to proteomic diversity, which is highly regulated in tissue- and development-specific patterns. Retinal tissue exhibits one of the highest levels of AS. In particular, photoreceptors have a distinctive AS pattern involving the inclusion of microexons not found in other cell types. PROM1 whose encoded protein Prominin-1 is located in photoreceptor outer segments (OSs), undergoes exon 4 inclusion from the 12th post-conception week of human development through adulthood. Exon 4 skipping in PROM1 is associated with late-onset mild maculopathy, however its role in photoreceptor maturation and function is unknown. In this study retinal organoids, a valuable model system, were employed in combination with phosphorodiamidate morpholino oligos (PMOs) to assess the role of exon 4 AS in the development of human retina. Retinal organoids were treated with the PMOs for four weeks after which RT-PCR, western blotting and immunofluorescence analysis were performed to assess exon 4 exclusion and its impact on photoreceptors. The transcriptome of treated ROs was studied by bulk RNA-Seq. Our data demonstrate that 55% skipping of PROM1 exon 4 resulted in decreased Prominin-1 expression by 40%, abnormal accumulation of cones in the basal side of the retinal organoids as well as detectable cone photoreceptor cilium defects. Transcriptomic and western blot analyses revealed decreased expression of cone, inner segment and connecting cilium basal body markers, increased expression of genes associated with stress response and the ubiquitin-proteasome system, and downregulation of autophagy. Importantly, the use of retinal organoids provides a valuable platform to study AS and unravel disease mechanisms in a more physiologically relevant context, opening avenues for further research and potential therapeutic interventions. Together our data indicate that cones may be more sensitive to PROM1 exon 4 skipping and/or reduced Prominin-1 expression, corroborating the pathogenesis of late-onset mild maculopathy.
Social identity correlates of social media engagement before and after the 2022 Russian invasion of Ukraine
Authors: Kyrychenko Yara , Brik Tymofii , van der Linden Sander , Roozenbeek Jon 
Published: October 2024

AbstractDespite the global presence of social media platforms, the reasons why people like and share content are still poorly understood. We investigate how group identity mentions and expressions of ingroup solidarity and outgroup hostility in posts correlate with engagement on Ukrainian social media (i.e., shares, likes, and other reactions) before and after the 2022 Russian invasion of Ukraine. We use a dataset of 1.6 million posts from Ukrainian news source pages on Facebook and Twitter (currently X) and a geolocated sample of 149 thousand Ukrainian tweets. Before the 2022 Russian invasion, we observe that outgroup mentions in posts from news source pages are generally more strongly associated with engagement than negative, positive, and moral-emotional language. After the invasion, social identity mentions become less strongly associated with engagement. Moreover, post-invasion ingroup solidarity posts are strongly related to engagement, whereas posts expressing outgroup hostility show smaller associations. This is the case for both news and non-news social media data. Our correlational results suggest that signaling solidarity with one’s ingroup online is associated with more engagement than negativity about outgroups during intense periods of intergroup conflicts, at least in the context of the Russian-Ukrainian war.
Predicting the first steps of evolution in randomly assembled communities
Authors: McEnany John , Good Benjamin H. 
Published: October 2024

AbstractMicrobial communities can self-assemble into highly diverse states with predictable statistical properties. However, these initial states can be disrupted by rapid evolution of the resident strains. When a new mutation arises, it competes for resources with its parent strain and with the other species in the community. This interplay between ecology and evolution is difficult to capture with existing community assembly theory. Here, we introduce a mathematical framework for predicting the first steps of evolution in large randomly assembled communities that compete for substitutable resources. We show how the fitness effects of new mutations and the probability that they coexist with their parent depends on the size of the community, the saturation of its niches, and the metabolic overlap between its members. We find that successful mutations are often able to coexist with their parent strains, even in saturated communities with low niche availability. At the same time, these invading mutants often cause extinctions of metabolically distant species. Our results suggest that even small amounts of evolution can produce distinct genetic signatures in natural microbial communities.
Molecular architecture and functional dynamics of the pre-incision complex in nucleotide excision repair
Authors: Yu Jina , Yan Chunli , Paul Tanmoy , Brewer Lucas , Tsutakawa Susan E. , Tsai Chi-Lin , Hamdan Samir M. , Tainer John A. , Ivanov Ivaylo 
Published: October 2024

AbstractNucleotide excision repair (NER) is vital for genome integrity. Yet, our understanding of the complex NER protein machinery remains incomplete. Combining cryo-EM and XL-MS data with AlphaFold2 predictions, we build an integrative model of the NER pre-incision complex(PInC). Here TFIIH serves as a molecular ruler, defining the DNA bubble size and precisely positioning the XPG and XPF nucleases for incision. Using simulations and graph theoretical analyses, we unveil PInC’s assembly, global motions, and partitioning into dynamic communities. Remarkably, XPG caps XPD’s DNA-binding groove and bridges both junctions of the DNA bubble, suggesting a novel coordination mechanism of PInC’s dual incision. XPA rigging interlaces XPF/ERCC1 with RPA, XPD, XPB, and 5′ ssDNA, exposing XPA’s crucial role in licensing the XPF/ERCC1 incision. Mapping disease mutations onto our models reveals clustering into distinct mechanistic classes, elucidating xeroderma pigmentosum and Cockayne syndrome disease etiology.
Caudate nucleus volume in medicated and unmedicated patients with early- and adult-onset schizophrenia
Authors: Andreou Dimitrios , Jørgensen Kjetil Nordbø , Nerland Stener , Calkova Tereza , Mørch-Johnsen Lynn , Smelror Runar Elle , Wortinger Laura A. , Lundberg Mathias , Bohman Hannes , Myhre Anne Margrethe , Jönsson Erik G. , Andreassen Ole A. , Agartz Ingrid 
Published: October 2024

AbstractThe caudate nucleus is a part of the striatum, and striatal hyperdopaminergia is considered central to the pathophysiology of schizophrenia. How caudate volume is affected in schizophrenia and what role antipsychotics play remains unclear. In early-onset schizophrenia (EOS), where psychosis emerges during a neurodevelopmentally critical phase, the caudate may exhibit a heightened vulnerability to the effects of antipsychotic medications. We hypothesized effects of both antipsychotic medication use and age of onset on caudate in schizophrenia. We included adult patients with EOS (n = 83) and adult-onset schizophrenia (AOS) (n = 246), adult healthy controls (HC, n = 774), adolescent patients with non-affective psychosis (n = 56) and adolescent HC (n = 97). We obtained T1-weighted MRI scans using a 1.5T Siemens scanner and General Electric 3T scanners. In our main analysis, we tested for main and interaction effects of diagnosis and current antipsychotic medication use on caudate volume. Adult patients with EOS (p < 0.001) and AOS (p = 0.002) had both larger caudate than HC. Age of onset (EOS/AOS) interacted with antipsychotic use (p = 0.004) which was associated with larger caudate in EOS (p < 0.001) but not in AOS (p = 0.654). Conversely, among medicated patients only, EOS had larger caudate than AOS (p < 0.001). No other subcortical structures showed differences between medicated EOS and AOS. Medicated adolescent patients with non-affective psychosis and medicated adult patients with EOS showed similar caudate volumes. The results may indicate a schizophrenia-related and a medication-induced caudate increase, the latter restricted to patients with EOS and possibly occurring already in adolescence shortly after disease onset.
Improved longevity of actomyosin in vitro motility assays for sustainable lab-on-a-chip applications
Authors: Melbacke Andreas , Salhotra Aseem , Ušaj Marko , Månsson Alf 
Published: October 2024

AbstractIn the in vitro motility assay (IVMA), actin filaments are observed while propelled by surface-adsorbed myosin motor fragments such as heavy meromyosin (HMM). In addition to fundamental studies, the IVMA is the basis for a range of lab-on-a-chip applications, e.g. transport of cargoes in nanofabricated channels in nanoseparation/biosensing or the solution of combinatorial mathematical problems in network-based biocomputation. In these applications, prolonged myosin function is critical as is the potential to repeatedly exchange experimental solutions without functional deterioration. We here elucidate key factors of importance in these regards. Our findings support a hypothesis that early deterioration in the IVMA is primarily due to oxygen entrance into in vitro motility assay flow cells. In the presence of a typically used oxygen scavenger mixture (glucose oxidase, glucose, and catalase), this leads to pH reduction by a glucose oxidase-catalyzed reaction between glucose and oxygen but also contributes to functional deterioration by other mechanisms. Our studies further demonstrate challenges associated with evaporation and loss of actin filaments with time. However, over 8 h at 21–26 °C, there is no significant surface desorption or denaturation of HMM if solutions are exchanged manually every 30 min. We arrive at an optimized protocol with repeated exchange of carefully degassed assay solution of 45 mM ionic strength, at 30 min intervals. This is sufficient to maintain the high-quality function in an IVMA over 8 h at 21–26 °C, provided that fresh actin filaments are re-supplied in connection with each assay solution exchange. Finally, we demonstrate adaptation to a microfluidic platform and identify challenges that remain to be solved for real lab-on-a-chip applications.
Assessment of environmental and health risks of potentially toxic elements associated with desert dust particles affected by industrial activities in Isfahan metropolitan
Authors: Yazdani Moslem , Karimzadeh Hamidreza , Azimzadeh Hamidreza , Soleimani Mohsen 
Published: October 2024

AbstractDust particles and their associated compounds can adversely affect human health and ecosystems. The aim of this study was to investigate the concentration, health, and ecological risks of selected potentially toxic elements (e.g. Pb, Cd, Cr, Co, Cu, Zn, V, Ni, and As) bound to air particles generated by dust storms in the Sejzi plain desert area within the industrial district of Isfahan metropolitan, Iran. The enrichment factor revealed the highest values for Zn, Pb, and Cd which among them Zn showed the highest value (8.1) with the potential source of industrial activities confirmed by the integrated pollution index, accumulation coefficient, and ecological risk index. Regarding health risk analysis (non-cancer and cancer risks) the elements including Co, As, and Cr showed a significant risk for adults and children across all seasons. It’s concluded that mitigation of air particles originated from both natural and industrial activities is necessary to reduce their relevant risks to human being and ecosystems in the region.
Improving I-ELM structure through optimal addition of hidden nodes: Compact I-ELM
Authors: Seo Sunghyo , Jo Jongkwon , Hamza Muhammad , Kim Youngsoon 
Published: October 2024

Abstract
Incremental extreme learning machines (I-ELMs) can automatically determine the structure of neural networks and achieve high learning speeds. However, during the process of adding hidden nodes, unnecessary hidden nodes that have little relevance to the target may be added. Several studies have proposed methods to overcome this problem by measuring the relevance between hidden nodes and outputs and adding or removing hidden nodes accordingly. Random hidden nodes have the advantage of creating diverse patterns, but they encounter a problem in which hidden nodes that generate patterns with little or no relevance to the target can be added, thereby increasing the number of hidden nodes. Unlike in existing I-ELMs, which use random hidden nodes, we propose a compact I-ELM algorithm that initially adds linear regression nodes and subsequently applies a method to ensure that the hidden nodes have patterns differing from the existing ones. Based on benchmark data, we confirmed that the proposed method constructs a compact neural network structure with fewer hidden nodes compared to the existing I-ELM systems.
EEG-based headset sleep wearable devices
Authors: Markov Karmen , Elgendi Mohamed , Menon Carlo 
Published: October 2024

AbstractThe rise of wearable technology has led to EEG-based sleep monitoring devices that use electrodes placed on the forehead, ear, or neck. These devices offer promising applications in clinical and healthy populations by comparing sleep patterns, monitoring intervention responses, and examining the relationship between sleep and lifestyle factors. Despite their potential, challenges like validation against polysomnography, regulatory hurdles, data privacy, and usability hinder clinical adoption. This review explores these devices, their applications, and integration challenges in clinical practice.
When No Speech Norms Exist: Observations From Sinhala
Authors: Hettiarachchi Shyamani , Ranaweera Mahishi , Saleem Shakeela , Krishnaveni Kanagendran 
Published: October 2024

Purpose:
A well-established set of language-specific norms for phonological development is imperative in the assessment of child speech sound difficulties. Currently, English norms are used clinically (in the absence of norms for local languages) to determine if a child displays age-appropriate, delayed or disordered speech patterns in Sinhala. This preliminary exploratory study aimed to document phonological processes observed in typically developing Sinhala-speaking children aged 3;0–6;11 (years;months).

Method:
The Test of Articulation and Phonology–Sinhala, a picture-based assessment, was devised by the researchers and administered to 102 Sinhala-speaking children from three geographical locations (Colombo, Kandy, and Gampaha). The quantitative measures included percent consonants correct, percent vowels correct, and percent phonemes correct, while the qualitative analysis identified phonological processes.

Results:
The quantitative results showed a marked influence of age on phoneme production accuracy with over 75% consonants correct by 3 years 6 months. The qualitative findings demonstrate common typical phonological processes and less common phonological processes in Sinhala compared to the speech pathology and cross-linguistic literature. Common phonological processes included fronting, stopping, and weak syllable deletion widely documented in linguistic and speech-language pathology literature. Many shared phonological processes were observed between Sinhala and Sri Lankan Tamil, the two main local languages, including fronting of retroflex sounds and lateralization. The phonological process of denasalization of prenasalized stops was observed in Sinhala, with no documentation of the phonological process found within the mainstream speech-language pathology literature.

Conclusion and Implications:
These findings reinforce the need to document and use language-specific typical phonological processes in Sinhala given the implications for early and accurate identification of speech difficulties and intervention.

Supplemental Material:

https://doi.org/10.23641/asha.27068173