bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE INFLUENCE OF METISEVIT AND METIFEN ON THE INTENSITY OF LIPID PER OXIDATION IN THE BLOOD OF BULLS ON NITRATE LOAD
Authors: Gutyj B.V. , Hufriy D.F. , Hunchak V.M. , Khariv I.I. , Levkivska N.D. , Huberuk V.О. 
Published: October 2016

The level of primary and secondary lipid per oxidation products were investigated: diene conjugates and malondialdehyde in conditions of nitrate loading. It was established that at bulls feeding with sodium nitrite at a dose of 0.2 hNO3ˉ/kg of body weight, the level of diene conjugates and malondialdehyde in their was increased during the entire the experiment. On the 30thday of the experiment the level of diene conjugates in blood of bull, which were conducted with nitrate load was 7.44 ± 0.15 mmol/l, and the level of malondialdehyde – 0.305 ± 0.014 mmol/l.Under conditions of nitrate load , young cattle was used a new integrated drug «Metisevit», which consists of sodium selenite, vitamin E and metifen. It was found the stimulating effect of metifen and metisevit on antioxidant system of the body of young cattle. Depressing effect of metifen and metisevit on the processes and lipid per oxidation in the blood of bulls under conditions of chronic nitrate–nitrite toxicity. Metifen and metisevit interact with radicals of fatty acids and delay the development of a chain reaction of oxidative stress, reduce the oxidation of phospholipids and form a biologically inactive compound with products of per oxidation of fats. Obtained results of the research indicate antioxidant drugs «Metisevit» and «Metifen» in the application of their young cattle.The mentioned changes are occurring through the comprehensive action of the drug components «Medisvit», that leads to the normalization of metabolic processes and free radical in the body of bulls. Obtained results of the research indicate the antioxidant action of the drugs «Metisevit» and «Metifen» in the application of their young cattle and the reasonableness of their administration to improve the antioxidant status of the organism according to nitrate loading.
ПОРІВНЯЛЬНА ОЦІНКА КОПРОСКОПІЧНИХ МЕТОДІВ ДІАГНОСТИКИ КРИПТОСПОРИДІОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ
Authors: Danko M.M. , Tishyn O.L. , Khomyak R.V. 
Published: October 2016

Cryptosporidiosis in cattle is a significant problem in most countries with intensive livestock, causing significant economic losses. The disease manifests appetite loss, depression, diarrhea, decreased growth, weight loss, retarded growth, and in some cases – death of sick animals. Equally important in the complex treatment and prevention of bovine cryptosporidiosis owned laboratory diagnostics, which allows early disease promptly detect and identify the pathogen. The article presents data on comparative assessment of three coprological methods of laboratory diagnosis of cryptosporidiosis in cattle. For this purpose, selected 10 samples of feces from calves infected with Cryptosporidium parvum, which added saline and were centrifuged. From the resulting suspension made smears and painted over by azure–eosin (by Giemsa) karbol–fuchsin (by Ziehl–Neelsen) safranine (by Kester). The results of the research identifying the lowest Cryptosporidium oocysts obtained by staining smears azure–eosin. Most effective method of detecting Cryptosporidium oocysts in cattle was staining method strokes by Kester. Average Cryptosporidium oocysts detected by this method was 1.9 times higher than Giemsa method and by 1.4 times – than Ziehl–Neelsen method.
ОСОБЛИВОСТІ БУДОВИ КІСТОК ТАЗОСТЕГНОВОГО СУГЛОБА ПТАХІВ, ЯК ОКРЕМОЇ ЛАНКИ ЛОКОМОТОРНОГО АПАРАТА
Authors: Druz N.V. 
Published: October 2016

This article is dedicated to the study of the structure, ways of formation of bone structures that form the basis of locomotor organs, namely the hip joint, the elucidation of mechanisms of their development, because they provide their reliable functioning. The study of the structural features of the skeleton of birds in comparison to other animals makes it possible to understand the phylogeny as an adaptive process that is the basis of evolution at all.Theoretical generalization of structural features of birds’ hip joint that are characterized by different types of biomorphological adaptations, such as the type and speed of ground movement in the habitat, are presented in the article. This new position allows analyzing the processes of differentiation and transformation of muscles and skeletal elements of birds’ hip joint, which are functioning and developing under the influence of various external factors.The summarized results of the original systematic morpho–functional and morpho–ecological study of hip bones as the main unit of bipedal locomotion of the Class Aves, is given. A detailed comparative description of skeletal elements of birds’ hip joint, that accompanied by unique historical overview which covers more than two–thousand–year period, is provided for the first time. The analysis of some significant morphological structures, which gives clues to the reconstruction of adaptive evolution of any group of birds, is given.
КОРЕКЦІЯ СТАНУ АНТИОКСИДАНТНОГО ЗАХИСТУ У СОБАК ЗА ОТРУЄННЯ НЕОВЕРМОМ
Authors: Zhukova I.O. , Svitlychna–Kulak Yu.S. , Longus N.I. 
Published: October 2016

The data on the impact of the drug «Neoverm» on the dogs have been presented in the article. The drug was administered into the stomach at the dose of 0.6 mg/kg of live weight for three days and the antioxidant drugs «E–Selenium» and «Quercetin» as well as green buckwheat and hepaprotector «L’esphal» as natural antioxidant preparations were used. «Neoverm» is a new antiparasitic drug that belongs to the group of medicines of broad–spectrum of action of the avermectin derivatives.The main active substance is ivermectin (10 mg), it belongsto the group of compounds which are the product of enzymic activity of the mycelium Streptomycesavermitilis. Ivermectinentersthebody of the parasite with the wasteproducts, it stimulates theproductionofneuromediator of inhibition – gamma–aminooleicacid that leadsto the disruptionofnerveimpulsetransmission, paralysisanddeathoftheparasite. The drug isused to treat varioustypesofdomesticanimalsandpoultry.On the basis of the results of the researches it has been established that the use of the drug «Neoverm» as a therapeutic agent alongwith «E–selenium», «Quercetin» and «L’esphal» and the correction of the ration with natural antioxidants has led to the gradual normalization of the processes of lipid peroxidation (LPO)in the blood of dogs, it was accompaniedby the significant (p ≤ 0.01) decrease in the formation of primary and final toxic by–products: dien conjugates (DC) and malonicdialdehyde (MDA) as well as the restoration of endogenous antioxidant resources (normalization of the activity of the enzyme catalase – the main indicator of the work of antioxidant system, the level of total antioxidant activity (AOA), the increase in the concentration of structural components of non–fermentative part of the antioxidant system (AOS) –vitamin E and Selenium).It has been pointed out that both correction schemes (the combination of «E–selenium» with «Quercetin» and green buckwheat with «L‘espal» gave good results, that is the basis for the use of the above drugs in the protective action.
БАКТЕРІАЛЬНЕ ЗАБРУДНЕННЯ МОЛОКА ЗА РІЗНИХ ТЕМПЕРАТУР І ТЕРМІНІВ ЗБЕРІГАННЯ
Authors: Zazharska N.M. 
Published: October 2016

Research was conducted in the laboratory LILCO, Surgères, France. For the first experiment, 24 samples of cooled bulk tank goat milk were selected be transported within 2 – 3 hours at different temperatures. Then all samples were stored day at 4 °C. The indicators of bacterial contamination, fat, protein, freezing point, somatic cell count, urea were similar for different temperatures of transporting milk samples. Noted the big somatic cell count (> 2000 thousand / ml) at low bacterial contamination (19,6 × 103 CFU/mL) of goat milk. Samples of milk can be delivered to the laboratory for 2–3 hours at a temperature of 2, 10 or 20 °C if the milk immediately after milking cooled and stored in a tank at 4 °C.10 samples of cow's milk (non–cooled – 3 hours after milking, cooled – after a day) were also examined. Bacterial contamination of milk which has been cooled and being stored one day at 4 °C was in 4.6 times less (P < 0.01) than non–cooled milk, analyzed in 3 hours after milking. This proves that bacterial contamination of milk in Ukraine accordance with European requirements (up to 100 thousand. CFU/ml) is possible only when rapid cooling of milk after milking to 4 оС and storing it in the cooling tank.
ПАТОМОРФОЛОГІЧНІ ЗМІНИ В ПЕЧІНЦІ СОБАК ЗА ЕКСПЕРИМЕНТАЛЬНОГО САРКОЦИСТОЗУ
Authors: Zvorygina V.E. , Prus M.P. , Borysevich B.V. 
Published: October 2016

Вasic macro– and microscopic changes in the liver of dogs in case of experimental sarcocystosis were determined as a result of our studies. Three clinically healthy puppy aged 2 – 4 months who were subjected to infection by feeding within three days of minced beef hearts, affected by sarcocysts, were selected for the experiment. The animals were subjected to euthanasia at the 7th, 14th and 21st day after infection. Autopsy was performed by partial evisceration in the conventional sequence. To detect microscopic structure of the liver, slices of 7– 10 µm thickness, obtained by the sliding microtome, were stained by hematoxylin and eosin Karazi. For detection of the lipids, frozen sections of thickness 15 – 20 µm, stained by Sudan III, were produced by using freezing microtome. Morphometry was performed due to G. Avtandylov. As a result of the research it was established that liver of all dogs macroscopically had not changed in size, its edges were sometimes sharpened, areas of different sizes and shapes of bluish and gray colors were seen. Macroscopic changes in the liver of dogs in case of experimental sarcocystosis were uncharacteristic. Conducting histology research, venous stasis and total hard granular dystrophy of hepatocytes in liver were determined. In case of experimental sarcocystosis of dogs the possibility of gametogony and sporogony of the causative agent in the liver was established.
СТАН ГУМОРАЛЬНОЇ ЛАНКИ ІМУННОГО СТАТУСУ У БУГАЙЦІВ НА ВІДГОДІВЛІ ЗА ВПЛИВУ ВІТАМІНІВ ГРУПИ B (B1, B2, B5, B6, B10, B12)
Authors: Zmiya M.M. , Golovach P.I. 
Published: October 2016

In realization of the genetic productivity potential of different species of farm animals, an important place is given to full feeding. Insufficient supply of farm animals with individual vitamins has negative impact on the activity of the relevant enzyme systems, hormonal status, metabolism of nutrients, the state of the natural resistance of the various organs and organ systems, the processes of adaptation and productivity level.Numerical searches have shown that farm animals need in different vitamins depends on the type, age, sex, physiological state, the season, the level of productivity and others. According to some reports ruminants have been providing with water–soluble B vitamins by their rumen microbial synthesis accordingly it was recommended to rations setting for cattle, sheep and goats, along with nutrients and minerals only by carotene and vitamins D and E .Studies have shown that the addition to the diet of calves for fattening complex of B vitamins (В1, В2, В5, В6, В10, В12) in different doses generally positive effect on bactericidal, lisocim and complement activity of blood serum, immunoglobulin cell in bull fattening.Studies have shown that the addition to the diet of bull fattening balanced in nutrients and minerals and fat–soluble vitamins A, D, E complex of B vitamins (В1, В2, В5, В6, В10, В12) in appropriate doses generally positive effect on humoral immunity state, major changes are complement activity of blood serum, depends on the dose additionally entered the diet fattening bulls B vitamins.The biggest change on humoral immunity state of calves for fattening derived from animals 3rd D (B1 – 0.040; B2 – 0.06; B5 – 1.2; B6 – 0.25, B10 – 0.0030; B12 – 0.0006 mg/kg body weight) and 4th D (B1 – 0.070; B2 – 0.10; B5 – 2.0; B6 – 0.40; B10 – 0.0050; B12 – 0.0010 mg/kg body weight) groups, and the smallest – in calves 1th D (В1 –0.015; В2 – 0,03; В5 – 0.5; В6 – 0.10; В10 – 0.0012; В12 – 0.0002 mg/kg body weight) group.
ВІДМІННОСТІ СКЛАДУ КРОВІ ПЕРИФЕРИЧНИХ І ЦЕНТРАЛЬНИХ ВЕН У СВИНЕЙ
Authors: Kibkalo D.V. , Borovkov S.B. , Korenev N.I. , Borovkova V.N. , Popova Kh.A. 
Published: October 2016

Among laboratory researches in animals the most widespread is the common blood test that can determine hidden changes in organs and tissues, monitor the effectiveness of therapeutic and preventive measures to predict outcome of disease. The question of the legality comparing the results of blood analysis from central veins with rates that are developed from capillary and peripheral vessels blood is studied in the article. Researches were conducted in seven pigs of ukrainian white breed in age 2–4 months. Blood was collected in the morning before feeding by puncture of ear vein, orbital sinus and cranial vena cava. From each animal were picked out 3 blood samples. For taking blood were applied the vacuum blood collection systems. In blood were determined the number of erythrocytes, leukocytes and hemoglobin by conventional methods (Kibkalo et al., 2016). From the results of leukocyte profile the major differences were found in the number of eosinophils whose content was significantly higher (r≤ 0.001) in the blood of ear veins. Should be noted that this index was much higher than the norm, which is possible associated with the pathological process. But their level in blood of the cranial vena cava and eye sinus was normal. Therefore, this question needs further study. Also found significantly lower (r ≤ 0.01) level of lymphocytes in the blood from the ear vein. In the vena cava were not found monocytes and basophils unlike eye sinus and ear veins, in the last their detected more. Based on the foregoing, it can be noted that in the blood of peripheral veins are registered larger number of granulocytes, which in future will be tissue macrophages. Neutrophils, monocytes, basophils and eosinophils have the ability to attach to the capillaries and small vessels walls. So they are providing the cellular immunity. In blood of central vessels are more lymphocytes that provides humoral immunity in the bloodstream. Perspectives of the next studies will be comparing the hematological results that are realized on automatic hematology analyzer from ear vein, eye sinus and cranial vena cava from the same piglets.
ВМІСТ ЖИРНИХ КИСЛОТ В ЛІПІДАХ ФЕТАЛЬНИХ СТОВБУРОВИХ КЛІТИН КОТА
Authors: Kladnitskaya L.V. , Mazurkiewicz A.J. , Danchuk V.V. , Velichko S.V. , Midyk S.V. 
Published: October 2016

Defined content of fatty acids in lipids cat stem cells derived from fetal primary material. Fetal stem cells (FSCs) cat treated by culturing primary material in the CO2 incubator containing 5% CO2, at a temperature 37°С in DMEM medium with the addition of 15 – 20% fetal bulls serum and 1% antibiotic–antimycotic. When confluent monolayer reached 70 – 80%, the cells are removed from the culture dishes and held subcultivation to reduce the heterogeneity of culture. The resulting stem cells are tested for fatty acid content by gas–liquid chromatography.Determination of fatty acids in lipids fetal stem cells conducted under SOST ISO 5508–2001. Sample preparation was performed according to ISO 150 5509–2002 in our modification. A mixture of methyl esters of fatty acids were analyzed on the gas chromatograph Trace GC Ultra with flame ionization detector for capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was performed using a standard sample Supelco 37 Сomponent FAME Mix. Performed quantitative assessment by spectrum of crystal planes valuation peaks methylated derivatives LCD and determine their content as a percentage of the total content of all the LCD.Investigated that the lipids contained cat FSCs short–, medium– and long–chain fatty acids. In the lipid fetal stem cells found cat 18 Number fatty acids from saturated – most of palmitatе (34.53 ± 0.58%), with monounsaturated – оleate (20.20 ± 0.93%), with polyunsaturated – linoleic acid (6.27 ± 0.01%). Least composed of lipids of cells found cis–8.11.14–eykozatriyenovoyi acid (0.03 ± 0.01%).The total content of saturated fatty acids in the lipid cat FSCs is 67.75, unsaturated fatty acids – 32.25%. Saturation ratio is 2.10. Monoyenic fatty acids identified in the number of 23.19%, and polyenic – 9,06%. The index value n3 fatty acids to n6 in lipids cat FSCs is 0.35.
ПАТОМОРФОЛОГІЧНІ ЗМІНИ ЛЕГЕНЕВОЇ ТКАНИНИ ЗА ІНФЕКЦІЙНОГО ПЕРИТОНІТУ КОТІВ
Authors: Kotsiumbas G. , Pritsak V. , Khalaniia M. 
Published: October 2016

The article presents the results of pathoanatomical, pathohistological and histochemical researches of the lung tissue for exudative form of the spontaneous infectious peritonitis of cats. The results of researches allow us to analyze pathoanatomical, microstructural changes in lung tissue of cats in various forms of peritonitis infectious and to define the mechanism of the pathological process that cause lethal consequence.The pathoanatomical research of dead bodies of 4 cats has been carried out: a 3 year–old cat and 1 year and 2 month–old cat and 1 year and 5 month–old with symptoms of exudative pleurisy; a 2 year–old cat with clear signs of exudative peritonitis and pleurisy. Peritoneal fluid and lung tissue fragments for cytological and pathohistological examination were selected. At pathoanatomical autopsy accumulation in the thoracal, abdominal cavities and pericardial space fluid with the flakes of fibrin were revealed, thickening of the serous membranes, layering them fibrin. At the pathohistological research in lung tissue noted the consequences of hemodynamic disturbances in the form of multiple perivascular hemorrhage, interstitial pneumonia, atelectasis and emphysematous cells and signs of fibrinous pleurisy in the state organization have been noted. The hardest changes were developed in the structures of the vascular system and are characterized by the damage of vascular endothelium, the development of productive meso– and periarteritis, syndrome of disseminated intravascular coagulation, stasis and hemolysis of erythrocytes in small vessels of interalveolar septum leading to the oppression of hemocirculation. In lungs, the mononuclear–macrophage infiltration prevailed, especially in periarteritis zone, indicating the presence of productively–necrotic vasculitis.The development of the syndrome disseminated intravascular coagulation led to the formation of multiple micro–blood clots, aggregated cells in vascular channel, the presence of which has led to the development of thrombi and then hemorrhages. The blockade microcirculation, in their turn, led to tissue hypoxia, acidosis and, as a result, distrophic changes in the past. The discovered processes in the vascular system of the lung tissue caused the insufficient flow of blood, irreversible changes of homeostasis and a sharp decline in adaptive capacity.
ЗМІНИ БІЛКОВИХ ПОКАЗНИКІВ КРОВІ КОРОПА ЗА ВИКОРИСТАННЯ КОМПЛЕКСУ СИМБІОНТНИХ МІКРООРГАНІЗМІВ
Authors: Mazur T. , Garkusha I. 
Published: October 2016

In modern conditions the intensification of cultivation and fish farming industrial fish farming today is based on the principles of technological pipeline. One way of improving the technology of cultivation and breeding of fish and maintain normal physiological status is the use of probiotic microorganisms. Fruits included in their composition can produce different active substances disposed of harmful metabolic products provide an antagonistic effect on pathogens. It is known that the use of probiotics affect the cellular and biochemical composition of blood, including the performance level of protein in the blood. The purpose of these studies was to determine the impact of complex probiotic microorganism Bacillus subtillis and Lactobacillus acidophilus compared with the use of these monocultures of microorganisms on the blood protein fractions of common carp. Through monitoring of total protein in serum may receive the most accurate information about the immune status carp. It was found that the inclusion in the diet of carp probiotic complex consisting of Bacillus subtilis and Lactobacillus acidophilus vplyvayea more positive on blood levels of total protein and its fractions in the serum of carp than using the latest in a monoculture. Besides data indicate intensification of metabolic processes in the body of the fish. However, the increase in γ–globulins shows a positive effect probiotynoho complex on humoral immunity factor carp.
ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ У КРОВІ КУРЧАТ–БРОЙЛЕРІВ НА ТЛІ ВАКЦИНАЦІЇ ПРОТИ ХВОРОБИ НЮКАСЛА ТА ЗА ДІЇ ВІТАМІНІВ E ТА C
Authors: Romanovich L.V. , Kurtyak B.M. , Romanovich М.S. , Mudrak D.I. 
Published: October 2016

The article presents research data on indices of lipid peroxidation (TBA–active products and hydroperoxides lipids) in the blood of broiler chickens on the background of vaccination and disease Nyukasla for the actions of vitamins E and C.The study was conducted on three groups of 100 broiler birds each. Control group fed normal chicken feed. The first research group in addition to poultry feed said received – tocopherol acetate in an amount of 0.1 g/kg feed, the second – ascorbic acid 0.25 g/kg feed. The third research group chickens – tocopherol acetate and ascorbic acid at these doses. For research use blood that was in the chicken after decapitation at different ages: 11–, 27–, 34– and 41–day age. The results of performance of different age groups broiler chickens compared with the value of the index poultry 11–day age for vaccination, and a control group of birds.Studies have shown that the content of intermediate and final products of lipid peroxidation in the blood plasma of broilers depends on the age and period of immunization. Feeding high amounts of vitamin E and C in the composition of feed for broiler chickens caused a reduction (p < 0.05 – 0.001) content in plasma lipid hydroperoxides and TBA–active products. PAUL likely lower rates of broiler chickens research groups suggest reducing the negative impact of stress factors on their bodies on the background of vaccination against the disease Nyukasla. These changes were more pronounced in the blood of chickens, which in addition to the basic diet receiving tocopherol acetate and ascorbic acid.
ПОКАЗНИКИ ЛІПІДНОГО ОБМІНУ У КОРІВ ЗА РІЗНИХ ФІЗІОЛОГІЧНИХ СТАНІВ ТА ПЕРІОДІВ УТРИМАННЯ
Authors: Simonov M.R. , Vlizlo V.V. , Butsyak V.I. 
Published: October 2016

The aim of this research was to study the lipid metabolism in high-yielding dairy cows during different physiological states and maintenance periods. Study was conducted on two groups of animals of Ukrainian Black-and-White dairy breed. The first group was formed in the winter stall-feeding period, the second one during the grazing period. Blood samples were taken four times: the first during dry period, the second, third and fourth were withdrawn at the beginning, on the peak and at the end of lactation period. Results showed increased lipomobilization activity after calving caused on the one hand by growing requirements in metabolites for milk synthesis and on the other hand by inadequate dietary supply with metabolic energy. Highly significant increase of the content of triacylglycerols, total and esterified cholesterol and non-esterified fatty acids in blood of dairy cows was revealed. On the peak and at the end of lactation the level of indicated substances in blood gradually decreased. Influence of the period of the animals maintenance on lipid metabolism was established. For instance, after calving and on the peak of lactation serum levels of triacylglycerols, esterifies cholesterol and non-estherified fatty acids were significantly higher in comparison with such under the same physiological states but during grazing period of maintenance.