bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activity‐dependent plasticity of presynaptic GABAB receptors at parallel fiber synapses
Authors: Orts‐Del'Immagine Adeline , Pugh Jason R. 
Published: January 2018

AbstractParallel fiber synapses in the cerebellum express a wide range of presynaptic receptors. However, presynaptic receptor expression at individual parallel fiber synapses is quite heterogeneous, suggesting physiological mechanisms regulate presynaptic receptor expression. We investigated changes in presynaptic GABAB receptors at parallel fiber‐stellate cell synapses in acute cerebellar slices from juvenile mice. GABAB receptor‐mediated inhibition of excitatory postsynaptic currents (EPSCs) is remarkably diverse at these synapses, with transmitter release at some synapses inhibited by >50% and little or no inhibition at others. GABAB receptor‐mediated inhibition was significantly reduced following 4 Hz parallel fiber stimulation but not after stimulation at other frequencies. The reduction in GABAB receptor‐mediated inhibition was replicated by bath application of forskolin and blocked by application of a PKA inhibitor, suggesting activation of adenylyl cyclase and PKA are required. Immunolabeling for an extracellular domain of the GABAB2 subunit revealed reduced surface expression in the molecular layer after exposure to forskolin. GABAB receptor‐mediated inhibition of action potential evoked calcium transients in parallel fiber varicosities was also reduced following bath application of forskolin, confirming presynaptic receptors are responsible for the reduced EPSC inhibition. These data demonstrate that presynaptic GABAB receptor expression can be a plastic property of synapses, which may compliment other forms of synaptic plasticity. This opens the door to novel forms of receptor plasticity previously confined primarily to postsynaptic receptors.
Seismic behavior of tall buildings using steel–concrete composite columns and shear walls
Authors: Ren Xiaodan , Bai Qiong , Yang Chengdong , Li Jie 
Published: January 2018

SummaryFor the seismic design of tall building structures, the behavior under severe earthquakes should be carefully considered and the upper limit of inter‐story deformations are often defined by the design codes. To improve the performance of structures under severe earthquakes, composite structural members, including steel reinforced column and steel plate reinforced shear wall, are often adopted. In the present work, the seismic behavior of tall buildings using steel–concrete composite columns and shear walls is investigated numerically. Fiber beam–column element models and multilayer shell models are adopted to establish the finite element model of structure, and the material nonlinearities are described by the plasticity and damage models. The accuracy of the developed models is verified by the experimental results of a single shear wall. Systematic numerical simulations are performed for the tall building structures subjected to different earthquakes. The comparative study indicates that the nonlinear redistribution of internal forces plays a very important role for the performance of tall buildings under severe earthquakes.
The role of the clinician in the multi‐omics era: are you ready?
Authors: van Karnebeek Clara D. M. , Wortmann Saskia B. , Tarailo‐Graovac Maja , Langeveld Mirjam , Ferreira Carlos R. , van de Kamp Jiddeke M. , Hollak Carla E. , Wasserman Wyeth W. , Waterham Hans R. , Wevers Ron A. , Haack Tobias B. , Wanders Ronald J.A. , Boycott Kym M. 
Published: January 2018

AbstractSince Garrod's first description of alkaptonuria in 1902, and newborn screening for phenylketonuria introduced in the 1960s, P4 medicine (preventive, predictive, personalized, and participatory) has been a reality for the clinician serving patients with inherited metabolic diseases. The era of high‐throughput technologies promises to accelerate its scale dramatically. Genomics, transcriptomics, epigenomics, proteomics, glycomics, metabolomics, and lipidomics offer an amazing opportunity for holistic investigation and contextual pathophysiologic understanding of inherited metabolic diseases for precise diagnosis and tailored treatment. While each of the ‐omics technologies is important to systems biology, some are more mature than others. Exome sequencing is emerging as a reimbursed test in clinics around the world, and untargeted metabolomics has the potential to serve as a single biochemical testing platform. The challenge lies in the integration and cautious interpretation of these big data, with translation into clinically meaningful information and/or action for our patients. A daunting but exciting task for the clinician; we provide clinical cases to illustrate the importance of his/her role as the connector between physicians, laboratory experts and researchers in the basic, computer, and clinical sciences. Open collaborations, data sharing, functional assays, and model organisms play a key role in the validation of ‐omics discoveries. Having all the right expertise at the table when discussing the diagnostic approach and individualized management plan according to the information yielded by ‐omics investigations (e.g., actionable mutations, novel therapeutic interventions), is the stepping stone of P4 medicine. Patient participation and the adjustment of the medical team's plan to his/her and the family's wishes most certainly is the capstone. Are you ready?
Understanding Organisational Ability and Self-Regulation in Children with Developmental Coordination Disorder
Authors: Green Dido , Payne Sally 
Published: January 2018

Abstract
Purpose of the Review
This paper examines physical and emotional coherence in young people with developmental coordination disorder (DCD). Specifically, the transactional relationship between motor and non-motor/executive dysfunction in children with DCD and its impact on psychosocial functioning is explored.

Recent Findings
This paper integrates the subjective reported experiences of young people with DCD with objective measurements and findings from neuroimaging studies.

Summary
Consideration of the transactional relationship between the person, the activity and the environment, whether these factors be physical, social, attitudinal or virtual, will be fundamental to our understanding of the mechanisms underpinning organisational and emotional issues presenting in DCD. Integrating the experiences of young people with research evidence will be essential to improve outcomes for young people with DCD in clinical practice.
Double sequential cardioversion for refractory ventricular tachycardia: A case report
Authors: Sheikh Hasan , Xie Edward , Austin Emily 
Published: January 2018

ABSTRACTSustained monomorphic ventricular tachycardia (VT) can result in hypoperfusion or devolve into more dangerous rhythms such as ventricular fibrillation. In an unstable patient with VT and a pulse, synchronized cardioversion is the first-line treatment. When the VT is refractory to standard cardioversion, the next step is to add an antiarrhythmic, such as amiodarone, that carries with it the risk of lowering blood pressure in the already hypotensive patient. Here we describe a case of double sequential synchronized cardioversion of a patient with unstable VT refractory to standard direct current cardioversion, resulting in a rapid conversion to sinus rhythm and return to hemodynamic stability. The benefit of this technique is that it may obviate the need for rapid infusion of medications, such as amiodarone, in the acute setting that may worsen hypotension in the already unstable patient.
Temporal evolution of the degree distribution of alters in growing networks
Authors: FOTOUHI BABAK , RABBAT MICHAEL 
Published: January 2018

AbstractThe degree distribution of the neighbors of nodes in a network is a theoretically important tool that is invoked in diverse studies in network science, such as epidemics, network resilience, network search and observability, network synchronization, random walks, opinion dynamics, and other dynamical systems on networks. Many real networks grow, and their properties pertaining to the said phenomena evolve. There is a paucity of theoretical research on how the evolution of these properties depend upon time and upon the structure of the initial network. This paper addresses this problem by providing the first theoretical study of the temporal evolution of the nearest-neighbor degree distribution for arbitrary networks (with any size) in arbitrary times. The posited results enable the analysis of the structural properties of growing networks in the short-time and intermediary time regimes, which are typically ignored in favor of the steady state. We corroborate the solutions via Monte Carlo simulations on various topologies. As a byproduct of the obtained solutions, we also demonstrate that the existing result in the literature on the asymptotic behavior of the Pearson coefficient of growing networks under the preferential attachment mechanism is incorrect, and we present the correct solution.
Justice and Mercy: An Enigmatic Yet Crucial Relationship for the Application of Canon Law
Authors: Schüller Thomas 
Published: January 2018

Not a single day goes by without Pope Francis making charity and mercy – central characteristic traits of any Christian – the pivot of his sermons, exhortations and proclamations, formal and informal alike. The last holy year, concluding in November 2016, which explicitly featured the motto of mercy, offers a welcome opportunity for the canonist to contemplate the relationship between mercy and justice. Certainly, there is no lack of warning speeches and auguries against the oversimplification of the canonical norms through invoking a false understanding of an imperative of indefinite mercy. To do so would be to neglect the crucial and central commandment of justice, which ensures both legal certainty and an equal treatment of similar cases. The papal address given to the Tribunal of the Roman Rota in 2009 may serve as an eloquent example. In his address Benedict XVI emphasised the interrelation of love (caritas) and justice (iustitia), but at the same time explicitly cautioned against over-pastorally motivated charity as far as the application of the law is concerned:
Charity without justice is not charity, but a counterfeit, because charity itself requires that objectivity which is typical of justice and which must not be confused with inhuman coldness. In this regard, as my Predecessor, Venerable Pope John Paul II, said in his Address on the relationship between pastoral care and the law: ‘The judge … must always guard against the risk of misplaced compassion, which could degenerate into sentimentality, itself pastoral only in appearance.’
There is sufficient reason, therefore, to consider once more how mercy and justice relate to one another.
Decryption of messages from extraterrestrial intelligence using the power of social media – The SETI Decrypt Challenge
Authors: Heller René 
Published: January 2018

AbstractWith the advent of modern astronomy, humans might now have acquired the technological and intellectual requirements to communicate with other intelligent beings beyond the solar system, if they exist. Radio signals have been identified as a means for interstellar communication about 60 years ago. And the Square Kilometer Array will be capable of detecting extrasolar radio sources analogous to terrestrial high-power radars out to several tens of light years. The ultimate question is: will we be able to understand the message or, vice versa, if we submit a message to extraterrestrial intelligence first, how can we make sure that they will understand us? Here I report on the largest blind experiment of a pretend radio message received on Earth from beyond the solar system. I posted a sequence of about two million binary digits (‘0’ and ‘1’) to the social media that encoded a configuration frame, two slides with mathematical content and four images along with spatial and temporal information about their contents. Six questions were asked that would need to be answered to document the successful decryption of the message. Within a month after the posting, over 300 replies were received in total, including comments and requests for hints, 66 of which contained the correct solutions. About half of the solutions were derived fully independently, the other half profited from public online discussions and spoilers. This experiment demonstrates the power of the world wide web to help interpreting possible future messages from extraterrestrial intelligence and to test the decryptability of our own deliberate interstellar messages.
Deposition rates of viruses and bacteria above the atmospheric boundary layer
Authors: Reche Isabel , D’Orta Gaetano , Mladenov Natalie , Winget Danielle M , Suttle Curtis A 
Published: January 2018

Abstract
Aerosolization of soil-dust and organic aggregates in sea spray facilitates the long-range transport of bacteria, and likely viruses across the free atmosphere. Although long-distance transport occurs, there are many uncertainties associated with their deposition rates. Here, we demonstrate that even in pristine environments, above the atmospheric boundary layer, the downward flux of viruses ranged from 0.26 × 109 to &gt;7 × 109 m−2 per day. These deposition rates were 9–461 times greater than the rates for bacteria, which ranged from 0.3 × 107 to &gt;8 × 107 m−2 per day. The highest relative deposition rates for viruses were associated with atmospheric transport from marine rather than terrestrial sources. Deposition rates of bacteria were significantly higher during rain events and Saharan dust intrusions, whereas, rainfall did not significantly influence virus deposition. Virus deposition rates were positively correlated with organic aerosols &lt;0.7 μm, whereas, bacteria were primarily associated with organic aerosols &gt;0.7 μm, implying that viruses could have longer residence times in the atmosphere and, consequently, will be dispersed further. These results provide an explanation for enigmatic observations that viruses with very high genetic identity can be found in very distant and different environments.
A caspase-2-RFXANK interaction and its implication for MHC class II expression
Authors: Forsberg Jeremy , Li Xinge , Akpinar Birce , Salvatori Roger , Ott Martin , Zhivotovsky Boris , Olsson Magnus 
Published: January 2018

AbstractDespite recent achievements implicating caspase-2 in tumor suppression, the enzyme stands out from the apoptotic caspase family as a factor whose function requires further clarification. To specify enzyme characteristics through the definition of interacting proteins in apoptotic or non-apoptotic settings, a yeast 2-hybrid (Y2H) screen was performed using the full-length protein as bait. The current report describes the analysis of a captured prey and putative novel caspase-2 interacting factor, the regulatory factor X-associated ankyrin-containing protein (RFXANK), previously associated with CIITA, the transactivator regulating cell-type specificity and inducibility of MHC class II gene expression. The interaction between caspase-2 and RFXANK was verified by co-immunoprecipitations using both exogenous and endogenous proteins, where the latter approach suggested that binding of the components occurs in the cytoplasm. Cellular co-localization was confirmed by transfection of fluorescently conjugated proteins. Enhanced caspase-2 processing in RFXANK-overexpressing HEK293T cells treated with chemotherapeutic agents further supported Y2H data. Yet, no distinct differences with respect to MHC class II expression were observed in plasma membranes of antigen-presenting cells derived from wild type and caspase-2−/− mice. In contrast, increased levels of the total MHC class II protein was evident in protein lysates from caspase-2 RNAi-silenced leukemia cell lines and B-cells isolated from gene-targeted mice. Together, these data identify a novel caspase-2-interacting factor, RFXANK, and indicate a potential non-apoptotic role for the enzyme in the control of MHC class II gene regulation.
Mitochondria are transported along microtubules in membrane nanotubes to rescue distressed cardiomyocytes from apoptosis
Authors: Shen Jing , Zhang Jiang-Hui , Xiao Han , Wu Ji-Min , He Kang-Min , Lv Zhi-Zhen , Li Zi-Jian , Xu Ming , Zhang You-Yi 
Published: January 2018

AbstractMembrane nanotubes (MNTs) act as “highways” between cells to facilitate the transfer of multiple signals and play an important role in many diseases. Our previous work reported on the transfer of mitochondria via MNTs between cardiomyocytes (CMs) and cardiac myofibroblasts (MFs); however, the elucidation of the underlying mechanism and pathophysiological significance of this transfer requires additional study. In this study, we determined that the mean movement velocity of mitochondria in MNTs between CMs and MFs was approximately 17.5 ± 2.1 nm/s. Meanwhile, treatment with microtubule polymerisation inhibitors nocodazole or colcemid in cell culture decreased mitochondrial velocity, and knockdown of the microtubule motor protein kinesin family member 5B (KIF5B) led to a similar effect, indicating that mitochondrial movement was dependent on microtubules and the motor protein KIF5B. Furthermore, we showed that hypoxia/reoxygenation-induced CM apoptosis was attenuated by coculture with intact or hypoxia/reoxygenation-treated MFs, which transferred mitochondria to CMs. This rescue was prevented either by separating the cells using Transwell culture or by impairing mitochondrial transfer with nocodazole or colcemid treatment. In conclusion, as a novel means of intercellular communication, MNTs rescue distressed CMs from apoptosis by transporting mitochondria along microtubules via KIF5B.
Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress
Authors: Gao Ming , Chen Minjun , Li Changying , Xu Ming , Liu Yun , Cong Min , Sang Nan , Liu Sijin 
Published: January 2018

AbstractMetallothioneins (MTs) are known to protect cells against oxidative stress, especially providing protection against cadmium (Cd) toxicity in hepatocytes. There are various gene variants and pseudogenes for MTs; however, there is little understanding on the functions of those non-coding MT members that are known to be expressed as long non-coding RNAs (lncRNAs) nowadays. Different from most protein-coding MT members, MT1DP was here found that remarkably induced to provoke cytotoxicity in hepatocytes in response to Cd treatment. MT1DP exerted such a pro-apoptotic function in Cd-treated hepatocytes through interacting with two partners: RhoC and MT1H. On one hand, MT1DP interacted with RhoC protein to increase the latter’s stability by preventing lysosome-dependent protein degradation. Therefore, upon Cd stress, MT1DP/RhoC complex was quickly reinforced to activate RhoC-CCN1/2-AKT signaling and potentiate Ca2+ influx, leading to enhanced Cd uptake and elevated Cd toxicity. On the other hand, MT1H, a protein-coding member of the MT family with little known function, was found to quickly respond to Cd exposure along with MT1DP. Mechanistically, MT1H and MT1DP were uncovered to mutually protect each other through a reciprocal ceRNA mechanism, building up a positive feedback loop to enforce MT1DP-conducted signaling upon Cd exposure. Moreover, MT1DP was found to contribute much more to the activation of RhoC-CCN1/2-AKT signaling than MT1H. Considered together, we here unveiled a mystery whether a pseudogene within the MT family, MT1DP, has actual biological functions in regulating Cd-induced cellular defense. Our findings unearthed an important role of pseudogene MT1DP in calibrating the cellular machinery to switch the cellular defense to cytotoxicity through crosslinking an interplay between its two partners, namely MT1H and RhoC, under cadmium stress.
Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics
Authors: Scott Brandon L. , Sochacki Kem A. , Low-Nam Shalini T. , Bailey Elizabeth M. , Luu QuocAhn , Hor Amy , Dickey Andrea M. , Smith Steve , Kerkvliet Jason G. , Taraska Justin W. , Hoppe Adam D. 
Published: January 2018

AbstractClathrin-mediated endocytosis (CME) internalizes plasma membrane by reshaping small regions of the cell surface into spherical vesicles. The key mechanistic question of how coat assembly produces membrane curvature has been studied with molecular and cellular structural biology approaches, without direct visualization of the process in living cells; resulting in two competing models for membrane bending. Here we use polarized total internal reflection fluorescence microscopy (pol-TIRF) combined with electron, atomic force, and super-resolution optical microscopy to measure membrane curvature during CME. Surprisingly, coat assembly accommodates membrane bending concurrent with or after the assembly of the clathrin lattice. Once curvature began, CME proceeded to scission with robust timing. Four color pol-TIRF showed that CALM accumulated at high levels during membrane bending, implicating its auxiliary role in curvature generation. We conclude that clathrin-coat assembly is versatile and that multiple membrane-bending trajectories likely reflect the energetics of coat assembly relative to competing forces.
Anderson localization of a one-dimensional quantum walker
Authors: Derevyanko Stanislav 
Published: January 2018

AbstractWe study the evolution of a system performing a one-dimensional quantum walk in the presence of static phase disorder. The same model also describes the propagation of classical light pulses in photonic mesh lattices. We study the interplay between the coupling (i.e. the bias of the “quantum coin”) and disorder. We provide an exact analytical expression for the localization length for two limiting cases of strong and weak phase disorder. In all the cases of interest we supply numerical simulations for participation ratio, Lyapunov exponent and the return probability as functions of the coupling parameter.
Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression
Authors: Yagnik Darshna , Serafin Vlad , J. Shah Ajit 
Published: January 2018

AbstractThe global escalation in antibiotic resistance cases means alternative antimicrobials are essential. The aim of this study was to investigate the antimicrobial capacity of apple cider vinegar (ACV) against E. coli, S. aureus and C. albicans. The minimum dilution of ACV required for growth inhibition varied for each microbial species. For C. albicans, a 1/2 ACV had the strongest effect, S. aureus, a 1/25 dilution ACV was required, whereas for E-coli cultures, a 1/50 ACV dilution was required (p < 0.05). Monocyte co-culture with microbes alongside ACV resulted in dose dependent downregulation of inflammatory cytokines (TNFα, IL-6). Results are expressed as percentage decreases in cytokine secretion comparing ACV treated with non-ACV treated monocytes cultured with E-coli (TNFα, 99.2%; IL-6, 98%), S. aureus (TNFα, 90%; IL-6, 83%) and C. albicans (TNFα, 83.3%; IL-6, 90.1%) respectively. Proteomic analyses of microbes demonstrated that ACV impaired cell integrity, organelles and protein expression. ACV treatment resulted in an absence in expression of DNA starvation protein, citrate synthase, isocitrate and malate dehydrogenases in E-coli; chaperone protein DNak and ftsz in S. aureus and pyruvate kinase, 6-phosphogluconate dehydrogenase, fructose bisphosphate were among the enzymes absent in C.albican cultures. The results demonstrate ACV has multiple antimicrobial potential with clinical therapeutic implications.