bluuebunny/crossref_metadata_2025_split huggingface dataset:
A network analysis to compare biomarker profiles in patients with and without diabetes mellitus in acute heart failure
Authors: Sharma Abhinav , Demissei Biniyam G. , Tromp Jasper , Hillege Hans L. , Cleland John G. , O'Connor Christopher M. , Metra Marco , Ponikowski Piotr , Teerlink John R. , Davison Beth A. , Givertz Michael M. , Bloomfield Daniel M. , Dittrich Howard , van Veldhuisen Dirk J. , Cotter Gad , Ezekowitz Justin A. , Khan Mohsin A.F. , Voors Adriaan A. 
Published: June 2017

AbstractAimsIt is unclear whether distinct pathophysiological processes are present among patients with acute heart failure (AHF), with and without diabetes. Network analysis of biomarkers may identify correlative associations that reflect different pathophysiological pathways.Methods and resultsWe analysed a panel of 48 circulating biomarkers measured within 24 h of admission for AHF in a subset of patients enrolled in the PROTECT trial. In patients with and without diabetes, we performed a network analysis to identify correlations between measured biomarkers. Compared with patients without diabetes (n = 1111), those with diabetes (n = 922) had a higher prevalence of ischaemic heart disease and traditional coronary risk factors. After multivariable adjustment, patients with and without diabetes had significantly different levels of biomarkers across a spectrum of pathophysiological domains, including inflammation (TNFR‐1a, periostin), cardiomyocyte stretch (BNP), angiogenesis (VEGFR, angiogenin), and renal function (NGAL, KIM-1) (adjusted P‐value <0.05). Among patients with diabetes, network analysis revealed that periostin strongly clustered with C‐reactive protein and interleukin‐6. Furthermore, renal markers (creatinine and NGAL) closely associated with potassium and glucose. These findings were not seen among patients without diabetes.ConclusionPatients with AHF and diabetes, compared with those without diabetes, have distinct biomarker profiles. Network analysis suggests that cardiac remodelling, inflammation, and fibrosis are closely associated with each other in patients with diabetes. Furthermore, potassium levels may be sensitive to changes in renal function as reflected by the strong renal–potassium–glucose correlation. These findings were not seen among patients without diabetes and may suggest distinct pathophysiological processes among AHF patients with diabetes.
Production dynamics in relation to ontogenetic development and induction of genetic instability through in vitro approaches in Pelargonium graveolens: A potential essential oil crop of commercial significance
Authors: Pandith Shahzad A. , Dhar Niha , Wani Tareq A. , Razdan Sumeer , Bhat Wajid Waheed , Rana Satiander , Khan Shabnam , Verma Mahendra K. , Lattoo Surrinder K. 
Published: June 2017

AbstractRose‐scented geranium (Pelargonium graveolens L’ Hért.) is a fragrant and bushy essential oil bearing crop of significant industrial and commercial importance. In an attempt to overcome the constraints imposed by sexual sterility for genetic improvement and restricted environmental adaptability of Pelargonium, the present study was aimed at exploring new niches for its cultivation in northern most sub‐tropical part of India (Jammu). The flowering stage was found to be the optimum stage for harvesting in relation to essential oil quantity (0.16–0.18%, Fresh Weight Basis; FWB) and quality (geraniol 18% and citronellol 37%) during different phenophases of plant growth. Dynamics of essential oil turnover correlated well with the glandular trichome ontogeny using scanning electron microscopy (SEM). The present study also entailed induction of somaclonal variations via in vitro organogenesis. Organogenetic callus induction was achieved on B5 (Gamborg's) basal medium supplemented with kinetin/2,4‐dichlorophenoxyacetic acid (Kn/2,4‐D) and kinetin/indole‐3‐butyric acid (Kn/IBA). Frequency of callus induction ranged from 54–85%. Field established in vitro regenerated plants/somaclones displayed appreciable variability in fresh leaf biomass (365–2020 g, FWB), essential oil content (0.06%–0.26%) and quality (geraniol and citronellol as major constituents). To evaluate the genetic instability of calliclones, RAPD (Random Amplification of Polymorphic DNA) markers were used to assess the genetic basis of variability. Further, the identity of Pelargonium was confirmed by DNA bar‐code analyses using three plant based barcoding loci (ITS, rbcL and psbA/trnH). They may be used as a standard reference system for varietal identification and protection of Intellectual Property Rights (IPR). Overall, the present investigation demonstrates the feasibility of Pelargonium cultivation in sub‐tropical niches as a short duration winter crop, with desirable essential oil content and composition for commercial purposes. It also establishes a viable method for induction of variability for genetic amelioration of sexually sterile crop.
Soft tissue angiofibroma: Clinicopathologic, immunohistochemical and molecular analysis of 14 cases
Authors: Bekers Elise M , Groenen Patricia JTA , Verdijk Marian AJ , Raaijmakers‐van Geloof Winny L , Roepman Paul , Vink Robert , Gilhuijs Nathalie DB , van Gorp Joost M , Bovée Judith VMG , Creytens David H , Flanagan Adrienne M , Suurmeijer Albert JH , Mentzel Thomas , Arbajian Elsa , Flucke Uta 
Published: June 2017

AbstractSoft tissue angiofibroma is rare and has characteristic histomorphological and genetic features. For diagnostic purposes, there are no specific antibodies available. Fourteen lesions (6 females, 8 males; age range 7‐67 years) of the lower extremities (12) and trunk (2) were investigated by immunohistochemistry, including for the first time NCOA2. NCOA2 was also tested in a control group of other spindle cell lesions. The known fusion‐genes (AHRR‐NCOA2 and GTF2I‐NCOA2) were examined using RT‐PCR in order to evaluate their diagnostic value. Cases in which no fusion gene was detected were additionally analysed by RNA sequencing. All cases tested showed nuclear expression of NCOA2. However, this was not specific since other spindle cell neoplasms also expressed this marker in a high percentage of cases. Other variably positive markers were EMA, SMA, desmin and CD34. STAT6 was negative in the cases tested. By RT‐PCR for the most frequently observed fusions, an AHRR‐NCOA2 fusion transcript was found in 9/14 cases. GTF2I‐NCOA2 was not detected in the remaining cases (n = 3). RNA sequencing revealed three additional positive cases; two harbored a AHRR‐NCOA2 fusion and one case a novel GAB1‐ABL1 fusion. Two cases failed molecular analysis due to poor RNA quality. In conclusion, the AHRR‐NCOA2 fusion is a frequent finding in soft tissue angiofibroma, while GTF2I‐NCOA2 seems to be a rare genetic event. For the first time, we report a GAB1‐ABL1 fusion in a soft tissue angiofibroma of a child. Nuclear expression of NCOA2 is not discriminating when compared with other spindle cell neoplasms.
Relationship between pancreatic intraepithelial neoplasias, pancreatic ductal adenocarcinomas, and single nucleotide polymorphisms in autopsied elderly patients
Authors: Matsuda Yoko , Tanaka Masashi , Sawabe Motoji , Mori Seijiro , Muramatsu Masaaki , Mieno Makiko Naka , Furukawa Toru , Arai Tomio 
Published: June 2017

AbstractWe comparatively analyzed serially autopsied, elderly Japanese patients (n = 2205) with pancreatic intraepithelial neoplasias (PanINs) and pancreatic ductal adenocarcinomas (PDACs) on the basis of their pancreatic lesions, clinical information, and single nucleotide polymorphisms (SNPs). The incidence of PanIN‐1, −2, −3, and PDACs in these patients was 55%, 12%, 1.4%, and 2.4%, respectively. The occurrence of PanINs was associated with female sex, increasing age, and lower body mass index. We did not identify any common SNPs between PanINs and PDACs. There were no common SNPs associated with PanINs and PDACs between men and women. In previously reported pancreatic cancer‐associated SNPs, rs3790844 (NR5A2) showed a significant correlation with PDAC in our cohort. Six SNPs (rs7016880, rs10096633, rs10503669, rs12678919, rs17482753, rs328) that were correlated with blood lipid levels were associated with the risk for PDACs. Our data suggest that different clinicopathological characteristics and predispositions may affect pancreatic carcinogenesis in elderly Japanese patients.
Declining Rates of Hip Fracture in End-Stage Renal Disease: Analysis From the 2003–2011 Nationwide Inpatient Sample
Authors: Kim Sun Moon , Liu Sai , Long Jin , Montez-Rath Maria E , Leonard Mary B , Chertow Glenn M 
Published: June 2017

ABSTRACT

The incidence of hip fracture in patients with end-stage renal disease (ESRD) is considerably higher than that in the general age- and sex-matched population. Although medical therapy for chronic kidney disease mineral bone disorder (CKD-MBD) has changed considerably over the last decade, rates of hip fracture in the entire ESRD population have not been well-characterized. Herein, we evaluated temporal trends in rates of hip fracture, in-hospital mortality, and costs of associated hospital stay in ESRD. We identified hospitalizations for hip fracture from 2003 to 2011 using the Nationwide Inpatient Sample, a representative national database inclusive of all ages and payers. We incorporated data from the United States Renal Data System and the US Census to calculate population-specific rates. Between 2003 and 2011, we identified 47,510 hip fractures in the ESRD population. The overall rate of hip fracture was 10.04/1000 person-years. The rate was 3.73/1000 person-years in patients aged less than 65 years, and 20.97/1000 person-years in patients aged 65 or older. Age- and sex-standardized rates decreased by 12.6% from 2003 (10.23/1000 person-years; 95% confidence interval [CI], 7.99/1000 to 12.47/1000) to 2011 (8.94/1000 person-years; 95% CI, 7.12/1000 to 10.75/1000). Hip fracture rates over time were virtually identical in patients aged less than 65 years; however, rates decreased by 15.3% among patients aged 65 years or older; rates declined more rapidly in older women compared with older men (p for interaction = 0.047). In-hospital mortality rate after hip fracture operation declined by 26.7% from 2003 (8.6%; 95% CI, 6.8 to 10.4) to 2011 (6.3%; 95% CI, 4.9 to 7.7). In ESRD, age- and sex-standardized hip fracture rates and associated in-hospital mortality have declined substantially over the last decade. © 2017 American Society for Bone and Mineral Research.
TP73‐AS1 promotes breast cancer cell proliferation through miR‐200a‐mediated TFAM inhibition
Authors: Yao Jia , Xu Feng , Zhang Danhua , Yi Wenjun , Chen Xianyu , Chen Gannong , Zhou Enxiang 
Published: June 2017

AbstractP73 antisense RNA 1T (TP73‐AS1 or PDAM) is a long non‐coding RNA, which can regulate apoptosis through regulation of p53 signaling‐related anti‐apoptotic genes. An abnormal change of TP73‐AS1 expression was noticed in cancers. The effects of TP73‐AS1 in breast cancer (BC) growth and the underlying mechanism remain unclear so far. In the present study, the effect of TP73‐AS1 in BC cell lines and clinical tumor samples was detected so as to reveal its role and function. In the present study, TP73‐AS1 was specifically upregulated in BC tissues and BC cell lines and was correlated to a poorer prognosis in patients with BC. TP73‐AS1 knocking down suppressed human BC cell proliferation in vitro through regulation of TFAM. In our previous study, we demonstrated that miR‐200a inhibits BC cell proliferation through targeting TFAM; here we revealed that TP73‐AS1 could regulate miR‐200a through direct targeting. Moreover, TP73‐AS1 might compete with TFAM for miR‐200a binding thus to promote TFAM expression. Data from the present study revealed that TP73‐AS1 promoted BC cell proliferation through acting as a competing endogenous RNA (ceRNA) by sponging miR‐200a. In conclusion, we regarded TP73‐AS1 as an oncogenic lncRNA promoting BC cell proliferation and a potential target for human BC treatment.
Classification of pancreaticobiliary maljunction and clinical features in children
Authors: Urushihara Naoto , Hamada Yoshinori , Kamisawa Terumi , Fujii Hideki , Koshinaga Tsugumichi , Morotomi Yoshiki , Saito Takeshi , Itoi Takao , Kaneko Kenitiro , Fukuzawa Hiroaki , Ando Hisami 
Published: June 2017

AbstractBackgroundIn 2015, the Committee on Diagnostic Criteria of the Japanese Study Group on Pancreaticobiliary Maljunction (PBM) proposed a classification of PBM into four types: (A) stenotic type, (B) non‐stenotic type, (C) dilated channel type, and (D) complex type. To validate this classification and clarify the clinical features of the four types of PBM, a retrospective multicenter study was conducted.MethodsThe study group of 317 children with PBM was divided into the four types of PBM. Clinical features, preoperative complications, operations, and postoperative pancreatic complications were evaluated.ResultsAll patients underwent excision of the extrahepatic bile duct. In type A, the age was younger and there was a higher incidence of cystic dilatation. Non‐dilatation of the common bile duct was frequently seen in type B. Abdominal pain with hyperamylasemia was frequently seen in types B and C. In particular, the incidence of protein plugs and biliary perforation was high in type C (56.1% and 14.3%, respectively). The overall incidence of acute pancreatitis was 7.3%. Pancreatitis after excisional surgery was rare in the children in this study. Two patients with type D (0.6%) developed chronic pancreatitis postoperatively.ConclusionsThis proposed classification is simple and correlates well with clinical features.
In‐scan and scan–rescan assessment of LV in‐ and outflow volumes by 4D flow MRI versus 2D planimetry
Authors: Kamphuis Vivian P. , van der Palen Roel L.F. , de Koning Patrick J.H. , Elbaz Mohammed S.M. , van der Geest Rob J. , de Roos Albert , Roest Arno A.W. , Westenberg Jos J.M. 
Published: June 2017

PurposeTo evaluate the in‐scan and scan–rescan consistency of left ventricular (LV) in‐ and outflow assessment from 1) 2D planimetry; 2) 4D flow magnetic resonance imaging (MRI) with retrospective valve tracking, and 3) 4D flow MRI with particle tracing.Materials and MethodsTen healthy volunteers (age 27 ± 3 years) underwent multislice cine short‐axis planimetry and whole‐heart 4D flow MRI on a 3T MRI scanner twice with repositioning between the scans. LV in‐ and outflow was compared from 1) 2D planimetry; 2) 4D flow MRI with retrospective valve tracking over the mitral valve (MV) and aortic valve (AV), and 3) 4D flow MRI with particle tracing through forward and backward integration of velocity data.ResultsIn‐scan consistency between MV and AV flow volumes is excellent for both 4D flow MRI methods with r ≥ 0.95 (P ≤ 0.001). In‐scan AV and MV flow by retrospective valve tracking shows good to excellent correlations versus AV and MV flow by particle tracing (r ≥ 0.81, P ≤ 0.004). Scan–rescan SV assessment by 2D planimetry shows excellent reproducibility (intraclass correlation [ICC] = 0.98, P < 0.001, coefficient of variation [CV] = 7%). Scan–rescan MV and AV flow volume assessment by retrospective valve tracking shows strong reproducibility (ICCs ≥ 0.89, P ≤ 0.05, CVs = 12%), as well as by forward and backward particle tracing (ICCs ≥ 0.90, P ≤ 0.001, CVs ≤ 11%). Multicomponent particle tracing shows good scan–rescan reproducibility (ICCs ≥ 0.81, P ≤ 0.007, CVs ≤ 16%).ConclusionLV in‐ and outflow assessment by 2D planimetry and 4D flow MRI with retrospective valve tracking and particle tracing show good in‐scan consistency and strong scan–rescan reproducibility, which indicates that both 4D flow MRI methods are reliable and can be used clinically.Level of Evidence: 2Technical Efficacy Stage: 2J. Magn. Reson. Imaging 2018;47:511–522.
Histogram analyses of diffusion kurtosis indices and apparent diffusion coefficient in assessing liver regeneration after ALPPS and a comparative study with portal vein ligation
Authors: Sheng Ruo‐fan , Wang He‐qing , Jin Kai‐pu , Yang Li , Liu Hao , Ji Yuan , Fu Cai‐xia , Zeng Meng‐su 
Published: June 2017

PurposeTo investigate the value of diffusion kurtosis imaging (DKI) histogram analysis in assessing liver regeneration and the microstructure basis after associating liver partition and portal vein ligation for staged hepatectomy (ALPPS), in comparison with portal vein ligation (PVL).Materials and MethodsThirty rats were divided into the ALPPS, PVL, and control groups. Histograms of DKI using a 3T magnetic resonance imaging (MRI) scanner were performed for corrected apparent diffusion (D), kurtosis (K), and apparent diffusion coefficient (ADC). Mean, median, skewness, kurtosis, and the percentiles (5th, 25th, 50th, 75th, and 95th) were generated and compared, and radiologic–pathologic correlations were evaluated.ResultsThere were more significant volume increases of the right median lobe after ALPPS than PVL (P = 0.0304/0.0017). The ALPPS group had larger hepatocyte size (P = 0.009/0.000), higher Ki‐67 and hepatocyte growth factor expression (P = 0.001–0.036) compared with both PVL and control groups. Mean, median, 5th, 25th, 50th, 75th percentiles of D map in ALPPS were lower than the control group (P = 0.001–0.022). Skewness and 75th, 95th percentiles of K map in ALPPS were higher than the PVL group (P = 0.011–0.042). No differences existed in the ADC map between groups (P = 0.073–0.291). Mean, median, 5th, 25th, 50th percentiles of D map, and 5th percentile of K map showed significant correlations with hepatocyte size (r = −0.582 to –0.426); no significant correlations were found in ADC parameters (P = 0.460–0.934).ConclusionALPPS induced true accelerated liver hypertrophy, superior to that seen with PVL. Histogram analysis of diffusion kurtosis indices may provide added values in evaluating liver regeneration and the intrinsic microstructure basis after ALPPS in comparison with the standard monoexponential ADC.Level of Evidence: 3Technical Efficacy Stage: Stage 2J. Magn. Reson. Imaging 2018;47:729–736.
PET/MRI for evaluating subclinical inflammation of ulcerative colitis
Authors: Shih I‐Lun , Wei Shu‐Chen , Yen Rouh‐Fang , Chang Chin‐Chen , Ko Chi‐Lun , Lin Been‐Ren , Shun Chia‐Tung , Liu Kao‐Lang , Wong Jau‐Min , Chang Yeun‐Chung 
Published: June 2017

PurposeTo explore the utility of integrated positron emission tomography (PET) / magnetic resonance imaging (MRI) for evaluating subclinical inflammation in patients with ulcerative colitis (UC).Materials and MethodsThis prospective study was approved by the Institutional Review Board and informed consent was obtained. Between November 2015 and April 2016, 19 consecutive patients with UC in clinical remission were enrolled. These patients underwent 18F‐fluorodeoxyglucose PET/MRI (3T) and colonoscopy. Serum high‐sensitivity C‐reactive protein (hs‐CRP) and fecal calprotectin (FC) levels were also obtained. The findings of colonoscopy were graded using the Mayo endoscopic subscore. Quantitative (minimum apparent diffusion coefficient [ADCmin] and maximum standardized uptake value [SUVmax]), semiquantitative, and qualitative parameters of PET/MRI were evaluated and correlated with colonoscopic findings.ResultsIn per‐segment analysis, ADCmin was significantly lower and SUVmax and ratio of SUVmax to ADCmin were significantly higher in the colonic segments with active inflammation (Mayo endoscopic subscore ≥2) (P < 0.05). Qualitative MRI score, PET activity grade, and PET/MRI score were also significantly higher in the colonic segments with active inflammation (P < 0.05). Among these parameters, the ratio of SUVmax to ADCmin exhibited the highest area under the receiver operating characteristic curve (AUC) (0.763). In per‐patient analysis, the AUC of PET activity grade was 0.778, higher than those of hs‐CRP (0.589) and FC (0.722). Using a combined index of FC and PET, an even higher AUC (0.867) was achieved.ConclusionPET/MRI is a potentially useful tool in identifying subclinical inflammation in patients with UC.Level of Evidence: 1Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2018;47:737–745.
Heat wave and cold spell changes in Iberia for a future climate scenario
Authors: Pereira Susana Cardoso , Marta‐Almeida Martinho , Carvalho Ana Cristina , Rocha Alfredo 
Published: June 2017

ABSTRACTThis study investigates the impacts of climate change scenario on summer heat waves' (HWs) and winter cold spells' (CSs) characteristics for 12 locations over the Iberian Peninsula (IP). These characteristics are duration, recovery factor and intensity. Two future time slices of the chosen scenario are studied, namely, the periods 2046–2065 and 2081–2100 which are compared with a reference climate for the recent‐past (1986–2005). TheRCP8.5 greenhouse gas emission scenario is considered. The minimum and maximum daily temperature were obtained for these periods through regional model simulations using the Weather and Research Forecast (WRF) model forced with theMPI‐ESM‐LRmodel. The model was validated againstEOBSandSPAIN02datasets. The model shows 90th/10th percentile temperature (i.e. thresholds to identifyHW/CS) biases. Therefore,HW/CSnumbers and properties were evaluated using the model's respective thresholds.HW/CSnumbers and characteristics were also compared between the model andEOBSderived data. Probability density functions (PDFs) of the duration, recovery factor and intensity show significant changes in the mean and variance for the summerHWs. Differences, between future and recent‐past climate in the extremes are evaluated by the 95th percentile which show an increase in the duration and intensity of theHWsfor the future time slices. Very fewCSswere detectable in the mid‐term future (2046–2065) and none in the long‐term future (2080–2100), except for Barcelona. For most locations, theCSfor the future are of smaller duration and intensity. ThePDFof the recovery factor suggests smaller absolute differences between the minimum and maximum temperature during winter which is also confirmed by the percentile analysis. The increase in the duration and intensity ofHWsis greater in the long‐term future than in the mid‐term future, pointing for a warmerIPwith more and longerHWstowards the end of theXXIcentury.