bluuebunny/crossref_metadata_2025_split huggingface dataset:
Baculovirus-assisted Reovirus Infection in Monolayer and Spheroid Cultures of Glioma cells
Authors: Dautzenberg Iris J. C. , van den Hengel Sanne K. , de Vrij Jeroen , Ravesloot Lars , Cramer Steve J. , Hong Saw-See , van den Wollenberg Diana J. M. , Boulanger Pierre , Hoeben Rob C. 
Published: December 2017

AbstractThe mammalian orthoreovirus Type 3 Dearing has great potential as oncolytic agent in cancer therapy. One of the bottlenecks that hampers its antitumour efficacy in vivo is the limited tumour-cell infection and intratumoural distribution. This necessitates strategies to improve tumour penetration. In this study we employ the baculovirus Autographa californica multiple nucleopolyhedrovirus as a tool to expand the reovirus’ tropism and to improve its spread in three-dimensional tumour-cell spheroids. We generated a recombinant baculovirus expressing the cellular receptor for reovirus, the Junction Adhesion Molecule-A, on its envelope. Combining these Junction Adhesion Molecule-A-expressing baculoviruses with reovirus particles leads to the formation of biviral complexes. Exposure of the reovirus-resistant glioblastoma cell line U-118 MG to the baculovirus-reovirus complexes results in efficient reovirus infection, high reovirus yields, and significant reovirus-induced cytopathic effects. As compared to the reovirus-only incubations, the biviral complexes demonstrated improved penetration and increased cell killing of three-dimensional U-118 MG tumour spheroids. Our data demonstrate that reovirus can be delivered with increased efficiency into two- and three-dimensional tumour-cell cultures via coupling the reovirus particles to baculovirus. The identification of baculovirus’ capacity to penetrate into tumour tissue opens novel opportunities to improve cancer therapy by improved delivery of oncolytic viruses into tumours.
Association of plasma free amino acids with hyperuricemia in relation to diabetes mellitus, dyslipidemia, hypertension and metabolic syndrome
Authors: Mahbub MH , Yamaguchi Natsu , Takahashi Hidekazu , Hase Ryosuke , Ishimaru Yasutaka , Sunagawa Hiroshi , Amano Hiroki , Kobayashi-Miura Mikiko , Kanda Hideyuki , Fujita Yasuyuki , Yamamoto Hiroshi , Yamamoto Mai , Kikuchi Shinya , Ikeda Atsuko , Kageyama Naoko , Nakamura Mina , Tanabe Tsuyoshi 
Published: December 2017

AbstractPrevious studies demonstrated independent contributions of plasma free amino acids (PFAAs) and high uric acid (UA) concentrations to increased risks of lifestyle-related diseases (LSRDs), but the important associations between these factors and LSRDs remain unknown. We quantified PFAAs and UA amongst Japanese subjects without LSRDs (no-LSRD, n = 2805), and with diabetes mellitus (DM, n = 415), dyslipidemia (n = 3207), hypertension (n = 2736) and metabolic syndrome (MetS, n = 717). The concentrations of most amino acids differed significantly between the subjects with and without hyperuricemia (HU) and also between the no-LSRD and LSRD groups (p < 0.05 to 0.001). After adjustment, the logistic regression analyses revealed that lysine in DM, alanine, proline and tyrosine in dyslipidemia, histidine, lysine and ornithine in hypertension, and lysine and tyrosine in MetS demonstrated significant positive associations with HU among the patients with LSRDs only (p < 0.05 to 0.005). By contrast, arginine, asparagine and threonine showed significant inverse associations with HU in the no-LSRD group only (p < 0.05 to 0.01). For the first time, we provide evidence for distinct patterns of association between PFAAs and HU in LSRDs, and postulate the possibility of interplay between PFAAs and UA in their pathophysiology.
Stim2-Eb3 Association and Morphology of Dendritic Spines in Hippocampal Neurons
Authors: Pchitskaya Ekaterina , Kraskovskaya Nina , Chernyuk Daria , Popugaeva Elena , Zhang Hua , Vlasova Olga , Bezprozvanny Ilya 
Published: December 2017

AbstractMushroom spines form strong synaptic contacts and are essential for memory storage. We have previously demonstrated that neuronal store-operated calcium entry (nSOC) in hippocampal neurons is regulated by STIM2 protein. This pathway plays a key role in stability of mushroom spines and is compromised in different mice models of Alzheimer’s disease (AD). Actin was thought to be the sole cytoskeleton compartment presented in dendritic spines, however, recent studies demonstrated that dynamic microtubules with EB3 capped plus-ends transiently enter spines. We showed that STIM2 forms an endoplasmic reticulum (ER) Ca2+ -dependent complex with EB3 via Ser-x-Ile-Pro aminoacid motif and that disruption of STIM2-EB3 interaction resulted in loss of mushroom spines in hippocampal neurons. Overexpression of EB3 causes increase of mushroom spines fraction and is able to restore their deficiency in hippocampal neurons obtained from PS1-M146V-KI AD mouse model. STIM2 overexpression failed to restore mushroom dendritic spines after EB3 knockdown, while in contrast EB3 overexpression rescued loss of mushroom spines resulting from STIM2 depletion. We propose that EB3 is involved in regulation of dendritic spines morphology, in part due to its association with STIM2, and that modulation of EB3 expression is a potential way to overcome synaptic loss during AD.
Automated computer-based detection of encounter behaviours in groups of honeybees
Authors: Blut Christina , Crespi Alessandro , Mersch Danielle , Keller Laurent , Zhao Linlin , Kollmann Markus , Schellscheidt Benjamin , Fülber Carsten , Beye Martin 
Published: December 2017

AbstractHoneybees form societies in which thousands of members integrate their behaviours to act as a single functional unit. We have little knowledge on how the collaborative features are regulated by workers’ activities because we lack methods that enable collection of simultaneous and continuous behavioural information for each worker bee. In this study, we introduce the Bee Behavioral Annotation System (BBAS), which enables the automated detection of bees’ behaviours in small observation hives. Continuous information on position and orientation were obtained by marking worker bees with 2D barcodes in a small observation hive. We computed behavioural and social features from the tracking information to train a behaviour classifier for encounter behaviours (interaction of workers via antennation) using a machine learning-based system. The classifier correctly detected 93% of the encounter behaviours in a group of bees, whereas 13% of the falsely classified behaviours were unrelated to encounter behaviours. The possibility of building accurate classifiers for automatically annotating behaviours may allow for the examination of individual behaviours of worker bees in the social environments of small observation hives. We envisage that BBAS will be a powerful tool for detecting the effects of experimental manipulation of social attributes and sub-lethal effects of pesticides on behaviour.
Non-Newtonian Flow to the Theoretical Strength of Glasses via Impact Nanoindentation at Room Temperature
Authors: Zehnder Christoffer , Peltzer Jan-Niklas , Gibson James S. K.-L. , Möncke Doris , Korte-Kerzel Sandra 
Published: December 2017

AbstractIn many daily applications glasses are indispensable and novel applications demanding improved strength and crack resistance are appearing continuously. Up to now, the fundamental mechanical processes in glasses subjected to high strain rates at room temperature are largely unknown and thus guidelines for one of the major failure conditions of glass components are non-existent. Here, we elucidate this important regime for the first time using glasses ranging from a dense metallic glass to open fused silica by impact as well as quasi-static nanoindentation. We show that towards high strain rates, shear deformation becomes the dominant mechanism in all glasses accompanied by Non-Newtonian behaviour evident in a drop of viscosity with increasing rate covering eight orders of magnitude. All glasses converge to the same limit stress determined by the theoretical hardness, thus giving the first experimental and quantitative evidence that Non-Newtonian shear flow occurs at the theoretical strength at room temperature.
Harnessing the microbiomes of Brassica vegetables for health issues
Authors: Wassermann Birgit , Rybakova Daria , Müller Christina , Berg Gabriele 
Published: December 2017

AbstractPlant health is strongly connected with plants´ microbiome. In case of raw-eaten plants, the microbiome can also affect human health. To study potential impacts on health issues of both hosts, the microbiome composition of seven different Brassica vegetables, originating from different food processing pathways, was analyzed by a combined approach of amplicon sequencing, metagenomic mining and cultivation. All Brassica vegetables harbored a highly diverse microbiota as identified by 16S rRNA gene amplicon sequencing. The composition of the microbiota was found to be rather driven by the plant genotype than by the processing pathway. We characterized isolates with potential cancer-preventing properties by tracing myrosinase activity as well as isolates with biological control activity towards plant pathogens. We identified a novel strain with myrosinase activity and we found bacterial myrosinase genes to be enriched in rhizosphere and phyllosphere metagenomes of Brassica napus and Eruca sativa in comparison to the surrounding soil. Strains which were able to suppress plant pathogens were isolated from naturally processed vegetables and represent a substantial part (4.1%) of all vegetable microbiomes. Our results shed first light on the microbiome of edible plants and open the door to harnessing the Brassica microbiome for plant disease resistance and human health.
Tunable two-phonon higher-order sideband amplification in a quadratically coupled optomechanical system
Authors: Liu Shaopeng , Yang Wen-Xing , Shui Tao , Zhu Zhonghu , Chen Ai-Xi 
Published: December 2017

AbstractWe propose an efficient scheme for the controllable amplification of two-phonon higher-order sidebands in a quadratically coupled optomechanical system. In this scheme, a strong control field and a weak probe pulse are injected into the cavity, and the membrane located at the middle position of the cavity is driven resonantly by a weak coherent mechanical pump. Beyond the conventional linearized approximation, we derive analytical expressions for the output transmission of probe pulse and the amplitude of second-order sideband by adding the nonlinear coefficients into the Heisenberg-Langevin formalism. Using experimentally achievable parameters, we identify the conditions under which the mechanical pump and the frequency detuning of control field allow us to modify the transmission of probe pulse and improve the amplitude of two-phonon higher-order sideband generation beyond what is achievable in absence of the mechanical pump. Furthermore, we also find that the higher-order sideband generation depends sensitively on the phase of mechanical pump when the control field becomes strong. The present proposal offers a practical opportunity to design chip-scale optical communications and optical frequency combs.
Changes in hyaluronan deposition in the rat myenteric plexus after experimentally-induced colitis
Authors: Filpa Viviana , Bistoletti Michela , Caon Ilaria , Moro Elisabetta , Grimaldi Annalisa , Moretto Paola , Baj Andreina , Giron Maria Cecilia , Karousou Evgenia , Viola Manuela , Crema Francesca , Frigo Gianmario , Passi Alberto , Giaroni Cristina , Vigetti Davide 
Published: December 2017

AbstractMyenteric plexus alterations hamper gastrointestinal motor function during intestinal inflammation. Hyaluronan (HA), an extracellular matrix glycosaminoglycan involved in inflammatory responses, may play a role in this process. In the colon of control rats, HA-binding protein (HABP), was detected in myenteric neuron soma, perineuronal space and ganglia surfaces. Prominent hyaluronan synthase 2 (HAS2) staining was found in myenteric neuron cytoplasm, suggesting that myenteric neurons produce HA. In the myenteric plexus of rats with 2, 4-dinitrobenzene sulfonic (DNBS)-induced colitis HABP staining was altered in the perineuronal space, while both HABP staining and HA levels increased in themuscularis propria. HAS2 immunopositive myenteric neurons and HAS2 mRNA and protein levels also increased. Overall, these observations suggest that inflammation alters HA distribution and levels in the gut neuromuscular compartment. Such changes may contribute to alterations in the myenteric plexus.
Artemisinin und seine Derivate – eine neue Option für die Krebstherapie?
Authors: Naß Janine , Efferth Thomas 
Published: December 2017

ZusammenfassungArtemisinin und seine Derivate sind nicht nur hochwirksame Medikamente gegen Malaria. Zahlreiche präklinische und vorläufige klinische Daten deuten darauf hin, dass sie auch therapeutisches Potenzial für die Onkologie besitzen könnten. Artemisinin-basierte Medikamente induzieren oxidativen Stress und DNA-Schäden und hemmen die Angiogenese. Tumorzellen sterben durch diese Substanzen, indem sie verschiedene Formen des Zelltodes hervorrufen (Apoptose, Autophagie, Ferroptose). Zytostatika-resistente Tumorzellen werden durch Artemisinin und seine Derivate effektiv abgetötet. Artemisinin-Derivate zeigen mit verschiedenen Standard-Krebsmedikamenten, Radiotherapie und therapeutischen Antikörpern additive bis synergistische Therapieeffekte gegenüber Tumorzelllinien. Ergebnisse aus klinischen Studien zeigten positive Ergebnisse, jedoch sollte bedacht werden, dass die behandelten Gruppen bisher nur klein waren. Individuelle Heilversuche sollten nur von approbierten Ärzten vorgenommen werden. Bis zur routinemäßigen klinischen Anwendung von Artemisinin und seinen Derivaten in der Krebstherapie wird sicherlich noch einige Zeit vergehen. Die bisherigen Ergebnisse sind jedoch vielversprechend.
Krankenhausseelsorge heute: Offenheit – Professionalität – Zusammenarbeit
Authors: Kammerer Thomas 
Published: December 2017

ZusammenfassungKrankenhausseelsorge hat in Deutschland eine lange Tradition, ist aber seit vielen Jahren in einem deutlichen Wandel begriffen. Nicht mehr konfessionell christliche „Mitgliedersorge“, sondern professionelle spirituelle und menschliche Unterstützung für alle Patienten, aber auch das Personal im Krankenhaus sowie interprofessionelle Zusammenarbeit stehen im Fokus. Regelmäßige Besuche auf Stationen, ethische Beratung, peritraumatische Krisenintervention, Begleitung und Gestaltung von Abschieden sowie die Mitarbeit an der Kultur eines menschlichen und umfassend heilenden Krankenhauses sind Komponenten einer modernen Seelsorge im Umfeld der Institution Krankenhaus in einer multikulturellen, individuellen und vielfältigen Gesellschaft.
Short-Term Effects of Combined High-Intensity Strength and Sprint Interval Training on Anthropometric Characteristics and Physical Performance of Elite Team Handball Players
Authors: Hermassi Souhail , Chelly Mohamed , Fieseler Georg , Bartels Thomas , Schulze Stephan , Delank Karl-Stefan , Shepard Roy , Schwesig René 
Published: December 2017

AbstractMuscular strength and speed are critical physical factors in determining the technical and tactical skills of elite team handball players. This study thus investigated the effect of high-intensity muscular strength and sprint interval training (SIT) on lower limb explosive performance and anthropometric characteristics in 22 male handball athletes aged 20.2 ± 0.1 years. A training group (TG, n = 12) and a control group (CG, n = 10) were followed over 8 weeks parallel to regular handball training. The TG received combined additional high-intensity muscular strength and interval training twice per week during this period. The muscular training was comprised of 3 – 4 sets of 70 – 85 % of 1-RM (repetition maximum) of dynamic back half squat exercise; followed immediately by a short sprint program with 4, 5, and 6 maximal intensity repetitions of 30 m runs. Strength (1-RM of the half back-squat and bench press), first step (V1S), first 5 m (V5 m), squat jumps (SJ), counter movement jumps (CMJ) and the Modified Agility Test (MAT) were tested at the beginning and end of the study. Significant interaction effects (group × time) were observed for all parameters (η² range: 0.531 (MAT) to 0.829 (First 5 m)). All 10 parameters showed relevant intervention effects (d> 0.5) in the TG (highest intervention effect: CMJ: d = 4.05), the mean effect size being d = 1.85. In contrast, scores for the CG either remained unchanged or decreased (d = –0.24). We conclude that combined high-intensity strength and sprint interval training during the competitive season should be recommended to elite male handball players as a means of improving handball-related performance characteristics without compromising other critical aspects of the individual's performance.
Population is the main driver of war group size and conflict casualties
Authors: Oka Rahul C. , Kissel Marc , Golitko Mark , Sheridan Susan Guise , Kim Nam C. , Fuentes Agustín 
Published: December 2017

Significance
Recent views on violence emphasize the decline in proportions of war groups and casualties to populations over time and conclude that past small-scale societies were more violent than contemporary states. In this paper, we argue that these trends are better explained through scaling relationships between population and war group size and between war group size and conflict casualties. We test these relationships and develop measures of conflict investment and lethality that are applicable to societies across space and time. When scaling is accounted for, we find no difference in conflict investment or lethality between small-scale and state societies. Given the lack of population data for past societies, we caution against using archaeological cases of episodic conflicts to measure past violence.
When Gist and Familiarity Collide: Evidence From False Recognition in Younger and Older Adults
Authors: Anderson Nicole D , Martin Chris B , Czyzo Julia , Köhler Stefan 
Published: December 2017

Abstract

Objectives
Aging is associated with decreased recollection required to offset misleading effects of familiarity, as well as an increased mnemonic reliance on gist-based over detail-based information. We tested the novel hypothesis that age-related decrements in overriding familiarity can be eliminated under conditions in which gist-based information facilitates retrieval.

Method
Twenty-seven younger adults and 27 older adults viewed scenes from two categories in an incidental encoding phase. In a recognition phase, old scenes were intermixed with new scenes from the studied categories and an unstudied category, with each new scene reappearing after 4, 18, or 48 intervening scenes. Participants were to respond “yes” to old scenes, and “no” to new scenes, including their repetitions.

Results
Despite encoding the scenes similarly, older adults made more false endorsements of new and repeated new scenes from studied categories. Both groups, however, were equally unlikely to falsely recognize new and repeated new scenes from the unstudied category.

Discussion
When helpful gist and misleading familiarity collide, gist wins, and eliminates age-related increases in false recognition.
Antibiotic Resistance of Potential Otopathogens Isolated From Nasopharyngeal Flora of Children With Acute Otitis Media Before, During and After Pneumococcal Conjugate Vaccines Implementation
Authors: Rybak Alexis , Levy Corinne , Bonacorsi Stéphane , Béchet Stéphane , Vié le Sage François , Elbez Annie , Varon Emmanuelle , Cohen Robert 
Published: December 2017

Background:
To re-evaluate antibiotic strategies for acute otitis media (AOM) in young children, we analyzed the trends of nasopharyngeal carriage and antibiotic resistance of Streptococcus pneumoniae (Sp), Haemophilus influenzae (Hi) and Moraxella catarrhalis (Mc) isolated from young children with AOM during a 16-year period.

Methods:
This cross-sectional study analyzed from 2001 to 2016 the nasopharyngeal carriage of Sp, Hi and Mc of young children with AOM. Medical history and physical findings were reported. Periods were defined by pneumococcal conjugate vaccine (PCV) introduction. We separately analyzed the 13-valent PCV (PCV13) period, which started after 2013.

Results:
During the study, 12,973 children with AOM were enrolled by 138 pediatricians. By comparing the first and last PCV periods, the proportion of children in day care centers and that of AOM with conjunctivitis significantly increased. The proportion of penicillin nonsusceptible Sp carriage significantly decreased during the study, with 0.8% of penicillin-resistant strains isolated in the PCV13 period, but that of β-lactamase–producing Hi continually increased to 23.6% in 2016. The level of Mc β-lactamase–producing strains remained high (>97%). In the PCV13 period, the main predictors of β-lactamase–producing Hi carriage were conjunctivitis (adjusted odds ratio = 6.0, 95% confidence interval [4.7–7.7]) and attending a day care center (2.4 [1.7–3.5]).

Conclusions:
In the PCV13 period, the proportion of penicillin-resistant Sp carriage was very low and that of β-lactamase–producing Hi carriage did not exceed 20% among children with AOM and without conjunctivitis. Our results suggest that amoxicillin may remain the first-line antibiotic treatment for AOM in young children except for those with conjunctivitis, for which amoxicillin-clavulanate is still the best antibiotic.
Psychopathology in borderline intellectual functioning: a narrative review
Authors: Peña-Salazar Carlos , Arrufat Francesc , Santos Josep Manel , Novell Ramón , Valdés-Stauber Juan 
Published: December 2017

Purpose
Studies on psychiatric comorbidity in individuals with borderline intellectual functioning (BIF) are scarce, particularly with respect to certain diseases frequently observed in clinical practice. The purpose of this paper is to discuss the relevance of epidemiological research to psychiatric comorbidity in people with BIF.

Design/methodology/approach
Systematic searches of MEDLINE, EMBASE and Cochrane databases. Inclusion criteria: publications about BIF appearing between 1995 and 2017; epidemiological findings about comorbid mental disorders in individuals with BIF; and studies comparing BIF, mild intellectual disability (ID) and normal intellectual functioning. The discussion covers 24 of the 224 studies initially considered.

Findings
The most frequent psychiatric comorbidity reported was personality, post-traumatic as well as psychotic disorders, followed by psychosis, attention deficit and hyperactivity disorder, bipolar and sleep disorders. Individuals with BIF exhibit psychiatric comorbidity more frequently than individuals with normal intellectual functioning. Some psychiatric comorbidities were similarly prevalent in patients with BIF and those with mild or moderate ID; however, the prevalence was always higher in people with severe ID. Environmental factors, especially psychosocial adversity, seem to play an important mediating role. Pharmacotherapy is the most common treatment approach, including behavioural disorders.

Originality/value
This review of literature on mental disorders in people with BIF demonstrates the epidemiological relevance of psychiatric comorbidity, especially personality and post-traumatic disorders. Mental health professionals, general practitioners and other workers in outpatient settings have to be aware about the vulnerability and even fragility of people with BIF.