bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between internet addiction and socio-psychological adaptation in adolescents
Authors: Mitrofanov I. V. 
Published: October 2024

Introduction. Socio-psychological adaptation occurs when a person interacts with the social environment. You can see how the Internet space is replacing live communication with digital communication. Adolescents between the ages of 14 and 16 have increased rates of personality development, which arouses interest in this study. The paper analyzes the socio-psychological adaptation of Internet-addicted adolescents. Materials and Methods. The study involved 205 9th grade students, among them 44 % of male and 56 % of female students. The average age of the respondents is 15-16 years old. Psychological methods were used in the course of the study. The study was carried out on the basis of MOBU Secondary Educational School No. 10 named after Bely S. I. Sochi in the 2022/23 academic year. The scale of "Internet addiction" (S. Chen (CTAS), the questionnaire of "Socio-psychological adaptation" (K. Rogers, R. Diamond and adapted by T. V. Snegireva (1987); A.M. Parishioners (2005), preference questionnaire. Results. The results of the study. It was found that out of 205 adolescent students, 17 % have formed and sustained Internet-dependent behavior. The correlation analysis showed the presence of a relationship between Internet addiction and socio-psychological adaptation in adolescents. Teenagers who are addicted to the Internet have a tendency: to neurotic deviation, the presence of intrapersonal conflicts, complications in decision-making, rejection of others. The need for communication, the desire for dominance, the form of internal locus of control, adaptability to the requirements of society, self-esteem, time management and adaptability are reduced. Discussion and Conclusions. Based on the results of an empirical study, we can talk about the connection between Internet-dependent behavior and socio-psychological adaptation in adolescents.
Technology of organization and performance of professional (demonstration) exam (on the example of special (defectological) education and subject areas «Russian language and literature», «Art»)
Authors: Kusova M. L. , Semenova E. V. , Simbirtseva N. A. 
Published: October 2024

Introduction. The article presents the experience of conducting professional (demonstration) examination in Master's degree program as a tool for assessing the readiness of future teachers for professional activity. The article considers the mechanisms of assessing the readiness of master's degree graduates to work in the educational system through the prism of professional, subject, methodological, communicative and managerial competencies. Materials and Methods. The presented characteristic of the organization and conduct of professional (demonstration) examination is based on theoretical research methods: logical analysis, structural-functional method, discourse analysis. The basis is the interdisciplinary methodology that allows synthesizing approaches inherent in the organization of the educational process. The method of systematization and analysis of the existing experience in conducting professional demonstration examination was used in describing the methodology of technology. Results. The purpose of these materials is to summarize and systematize the experience of conducting professional examination of master's students at the Ural State Pedagogical University. The principles and stages of the professional (demonstration) examination developed by the Educational and Methodological Council of the Urals State Pedagogical University are presented as organizational bases of the activity of organizing and conducting the professional (demonstration) examination. The principles implemented in the professional (demonstration) examination, such as continuity and continuity of teacher training, inclusiveness of the educational environment, orientation to professional communication and group decision-making, the principle of openness, combination of fundamentality and practice-oriented are outlined. Discussion and Conclusions. The optimal form of conducting the teachers' demonstration exam is determined as an activity-type technology corresponding to its tasks, based on an active way of interaction between participants – a business game. In the course of the business game, master's students simulate the situation of a meeting of a methodological association or pedagogical council, where they realize the solution of a professional problem. Content aspects of the professional (demonstration) examination are revealed in the article on the material of specific illustrations of variants of examination tasks for Master's degree students in the areas of training «Pedagogical Education» (on the example of subject areas «Russian language and literature» and «Art») and «Special (defectological) education».
Formação e intensificação de sistemas causadores de precipitação e temperaturas mínimas extremas em todo o Brasil no fim do outono de 2022.
Authors: Mendes Paulo Vítor de Albuquerque 
Published: October 2024

A passagem de frentes frias entre o Sul e Nordeste do Brasil (NEB) provocou vários fenômenos adversos no fim do outono de 2022. Este estudo, a análise sinótica e termodinâmica da formação, intensificação e impactos dos sistemas e fenômenos associados às frentes frias entre Maio e o início de Junho de 2022. Foram identificados sete ciclones em atividade na América do Sul, cinco deles responsáveis por fenômenos adversos em todo o Brasil. Quatro extremidades frontais associadas a ciclones acoplados à Corrente de Jato Subtropical, Jato de Baixos Níveis e Zona de Convergência Intertropical atingiram a região costeira do NEB. Durante a passagem dos ciclones mais intensos foram identificadas precipitações de até 176,6 mm/dia, temperaturas de até -7,7 °C, rajadas de vento de até 28,6 m/s e 8 eventos de geada registrados pelas estações do INMET. Também foram observados acumulados mensais de precipitação acima das médias históricas para o mês de Maio em algumas regiões do Brasil, causados principalmente por CCMs em extremidades frontais próximas às estações de João Pessoa - PB (714,2 mm/mês), Recife - PE (786,3 mm/mês), Maceió - AL (579,0 mm/,ês), Cametá - PR (544,7 mm/mês) e Vacaria - RS (427,8 mm/mês).
International Approaches to the Development, Validation, and Change Management of Analytical Procedures (Review)
Authors: Fetisova A. V. , Aladysheva Zh. I. , Pyatigorskaya N. V. , Zyryanov O. A. , Marshalova M. M. 
Published: October 2024

INTRODUCTION. In 2023, the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) published a new Guideline on Analytical Procedure Development (ICH Q14) and a revised version of the Guideline on Validation of Analytical Procedures (ICH Q2(R2)). Consequently, there is a need for a considerable revision of the approach to the development and validation of analytical procedures that is currently used in the Eurasian Economic Union (EAEU). A revision is also needed for the processes for evaluating and introducing variations to the analytical procedures described in medicinal product registration dossiers.AIM. This review aimed to analyse the significant changes made to international approaches to the development of analytical procedures, as well as to study the advantages and disadvantages of these approaches for pharmaceutical manufacturers and regulatory agencies in the EAEU.DISCUSSION. This review covers the key provisions and practical aspects of the enhanced approaches to the development of analytical procedures introduced by the ICH Q14 guideline. In particular, the review addresses the concepts of the analytical procedure life cycle (APLC) and the modified analytical quality-by-design (AQbD) approach; the development of the analytical target profile (ATP); analytical quality risk management; planning of the design of experiments (DoE) and the analytical procedure control strategy; and the validation, subsequent verification, transfer, and change management of analytical procedures. Additionally, the review describes the ICH Q2(R2) updates that accompany this new regulatory paradigm.CONCLUSIONS. The above guidelines fill the existing gap in recommendations for the development of analytical procedures. The use of the APLC and AQbD concepts provides both pharmaceutical companies and regulatory authorities with flexible approaches that are applicable to analytical procedures both during the development phase and once they have been implemented. Effective implementation of these international approaches in the Russian pharmaceutical industry and regulatory system requires a broad discussion between pharmaceutical industry professionals and regulatory agency experts, possibly, as part of a pilot project. After that, there will be a necessity to provide training for specialists involved in the development of analytical procedures and to amend the EAEU Rules for Marketing Authorisation and Expert Assessment of Medicinal Products for Human Use.
Immunogenicity of various variants of Ebola and Marburg virus glycoprotein genes in recombinant adenoviral vectors
Authors: Ozharovskaia T. A. , Zubkova O. V. , Popova O. , Kovyrshina A. V. , Goldovskaya P. P. , Vavilova I. V. , Dolzhikova I. V. , Ermolova E. I. , Kunda M. S. , Ryzhova N. N. , Voronina O. L. , Shcherbinin D. N. , Shcheblyakov D. V. , Logunov D. Y. , Gintsburg A. L. 
Published: October 2024

INTRODUCTION. Marburg and Ebola viruses cause severe haemorrhagic fever in humans and primates. Currently, there are no licensed prophylactic vaccines that can simultaneously prevent the spread or reduce the severity of both diseases caused by these filoviruses. The development of effective prophylactic vaccines requires studies aimed at selecting the most immunogenic forms of protective antigens.AIM. This study aimed to evaluate humoral immune induction in animals after administration of recombinant adenoviral vectors expressing various forms of Ebola and Marburg virus glycoproteins (GPs).MATERIALS AND METHODS. Samples of recombinant human adenovirus type 5 (rAd5) were obtained using homologous recombination in Escherichia coli, growth in HEK293 cells, and purification by CsCl gradient ultracentrifugation. The resulting rAd5 samples were characterised in terms of their identity (PCR and whole-genome sequencing), the concentration of viral particles (fluorescence spectroscopy), and the concentration of infectious viral particles (TCID50 assay). Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the GP-specific IgG titres in the sera of immunised mice.RESULTS. The authors constructed rAd5 samples, and each construct contained an expression cassette with a GP gene form encoding a full-length GP, a GP without the mucin-like domain, or a GP without both the glycan cap and the mucin-like domain. Each of these forms was studied using the GPs of four filoviruses, including Zaire Ebola virus, Sudan Ebola virus, Bundibugyo Ebola virus, and Marburg virus. Neither of the forms had a critical effect on the rAd5 replicative capacity. Three weeks after immunisation, the highest GP-specific IgG production was induced by the rAd5 samples encoding either the full-length GP or the GP without the mucin-like domain. The GP without both the glycan cap and the mucin-like domain was the least immunogenic antigen regardless of the filovirus species.CONCLUSIONS. The most promising constructs for the development of filovirus vaccines based on recombinant adenoviral vectors are the constructs that include the genes encoding the fulllength GP or the GP without the mucin-like domain.
Study of the protective efficacy of CombiMab-2 against human immunodeficiency virus type 1 in mice humanised with CD4<sup>+</sup> T-lymphocytes
Authors: Leontyev D. S. , Urusov F. A. , Glazkova D. V. , Belugin B. V. , Orlova O. V. , Mintaev R. R. , Tsyganova G. M. , Bogoslovskaya E. V. , Shipulin G. A. 
Published: October 2024

INTRODUCTION. Despite existing treatment methods, complete eradication of human immunodeficiency virus (HIV) infection remains an unattainable goal due to the high variability of HIV type 1 (HIV-1). HIV infection necessitates life-long administration of antiretroviral medicinal products, which cause serious adverse drug reactions. The development of gene therapy products based on adeno-associated virus (AAV) vectors encoding broadly neutralising antibodies represents a promising direction for creating long-term therapies capable of countering a wide range of viral variants.AIM. This study aimed to evaluate the protective efficacy of CombiMab-2, a medicinal product consisting of a combination of three AAV vectors (AAV9-VRC07-523, AAV9-10-1074, and AAV9-PGDM1400) encoding broadly neutralising antibodies against HIV-1, in a humanised mouse model.MATERIALS AND METHODS. The study used an HIV infection model based on immunodeficient B-NDG mice humanised with human CD4+ lymphocytes (1.5×107 cells per animal) from a leukoconcentrate of a healthy donor. The experiment used two groups of mice, including a control group (3 animals) receiving saline solution and an experimental group (5 animals) receiving CombiMab-2. The medicinal product was administered into different muscles as three separate components six weeks prior to infection. The CCR5-tropic HIV-1 strain was obtained by transfecting HEK293FT cells with the pNL4-3(AD8) plasmid encoding the full-length virus. The authors monitored viral loads in the plasma of animals by reverse transcription polymerase chain reaction and CD4+ lymphocyte counts in the peripheral blood of animals by flow cytometry for four weeks after infection.RESULTS. Six weeks after CombiMab-2 administration, the levels of broadly neutralising antibodies in the serum of humanised mice ranged from 0.17 μg/mL to 4.0 μg/mL. In the control group, the viral load reached 105 copies/mL one week after HIV-1 infection and continued to rise over the next three weeks. In the treatment group, infection developed only in one mouse, which had the lowest antibody titre before infection. No viral load was detected in the remaining mice of the treatment group, which indicated that the medicinal product was effective if serum concentrations of broadly neutralising antibodies reached 0.5 μg/mL or higher.CONCLUSIONS. The tested medicinal product based on three AAV vectors (AAV9-VRC07-523, AAV9-10-1074, and AAV9-PGDM1400) exhibits protective activity against HIV-1 in humanised mice. The presented data allow the authors to consider CombiMab-2 as a promising antiviral agent that can serve as a basis for further pharmaceutical development.
Service Quality Affects BPJS Patient Satisfaction at the Pematang Health Center, Serang Regency, Banten in 2024
Authors: Kurniyanti Novia , Effendy Ismail , Anggraini Indah 
Published: October 2024

Health service providers must strive to improve the quality of services for the realization of the highest degree of public health. The purpose of this study is to find out and analyze the quality of service affecting BPJS patient satisfaction at the Pematang Health Center, Serang Regency, Banten in 2024. The research design uses an analytical survey with a cross sectional design. The population in this study is all patients who are treated using BPJS as many as 4,210 people with an accidental sampling technique with a sample of 98 people. The data analysis used used was univariate analysis, bivariate using chi-square and multivariate using logistic regression. The results of the logistic regression analysis that affected were the variables of immediate service p = 0.003, politeness p = 0.005 and effective communication p = 0.000 and the most influential variable was effective communication with an Exp B value of 13.922. The conclusion of the variables that do not affect is the use of information technology, physical facilities, quick response, skills and attitudes and the variables that affect are prompt service, politeness and effective communication and the most influential is effective communication. It is recommended to the Puskesmas to make a budget for Communication Skills training for all health workers, mentoring, and implementation by making the motto of the Pematang Health Center to be (smile, greeting, polite fast and precise). Providing services in a timely, polite, friendly manner and can maintain patient privacy in order to increase patient satisfaction and provide physical facilities such as (chairs, air conditioners, loudspeakers and machines to take no queue) as well as provide information on the use of mobile JKN.
Análisis del comercio intraindustrial entre Alemania, China, India y EE.UU, periodo 2009-2022
Authors: Norabuena Trejo Pierina 
Published: October 2024

Este artículo analiza el comercio intraindustrial entre Alemania, China, India y EE.UU. en el periodo 2009-2022. En este sentido, las variables de índice global de competitividad, el Índice de Globalización KOF y las exportaciones de alta tecnología (que explican el comercio) se convierten en indicadores clave para evaluar el desempeño de los países tecnológicamente avanzados. Los datos provienen del Banco Mundial, de los Datos Macro y del Instituto Económico Suizo, utilizando el modelo de datos de panel de efectos aleatorios, donde el índice global de competitividad, índice de globalización KOF y las exportaciones de alta tecnología tienen relaciones estadísticamente significativas con el comercio en un nivel de significancia del 5 %. Estos resultados se asocian con cambios en el comercio.
Travelling in Romantic Bildungsroman Narratives
Authors: Nivala Asko 
Published: October 2024

The early nineteenth-century bildungsroman is often understood as a coming-of-age story of the protagonist, which implies that temporality is more constitutive for its composition than spatiality. In contrast, my paper focuses on the motif of travelling in German and British bildungsroman narratives. Opposed to the previous allegorical understanding of the Romantic bildungsroman, I suggest that the methods of the spatial humanities open up a new perspective on bildungsroman narratives by showing how their travel itineraries were used in the construction of Europe before and after the Napoleonic Wars. The itineraries annotated from the corpus suggest that the classic Grand Tour to Italy remain important, especially in German Romanticism, whereas northern regions are also popular in British texts. A few narratives emphasise the region of Greece in defining the European frontier in relation to the Ottoman Empire, while Russia and Poland are not popular settings for bildungsroman narratives.
Digital sexual literacy: the potential for online spaces to support sexual literacy among young people
Authors: Power Jennifer , James Alexandra , Waling Andrea , Pym Tinonee , Lim Gene 
Published: October 2024

IntroductionHealth literacy is defined as the capacity, resources and insight to seek information, ask critical questions and be an advocate for one’s health. Sexual literacy is framed in similar terms, relating to the capacity to navigate emotionally and physically safe sexual encounters and to hold a critical and assertive stance on relationship dynamics, gender and consent. In this paper, we present findings from a qualitative study that explored how young people’s use of the internet develops sexual literacy.MethodsIn-depth interviews were conducted with 22 young people (aged 18–25). Interviews explored the ways in which young people participate in online spaces to learn about sex, relationships, bodies and other aspects of sexual health. Data were analysed using qualitative, inductive thematic coding techniques.ResultsYoung people described learning about sexual health online as a process of exploring ideas, topics or experiences (going down ‘rabbit holes’). It was rarely the case that young people searched for specific information. Young people described engaging with multiple sources of content and multiple people online to learn about relationships or sex. This was often personal. Young people wanted to understand their own experiences and hear stories from people with similar experiences. Engaging with multiple sources, stories and people enabled critical reflection and peer-based learning.DiscussionBuilding sexual health literacy is not simply about providing young people with sexual health information. Rather, it is about supporting young people to develop critical insight and capacity to reflect deeply on experiences and issues that affect their sexual relationships. The online environment supports sexual literacy as it enables critical inquiry, access to multiple perspectives, peer connection and personal reflection.
Estradiol and progesterone from pregnancy to postpartum: a longitudinal latent class analysis
Authors: Dukic Jelena , Johann Alexandra , Henninger Mirka , Ehlert Ulrike 
Published: October 2024

IntroductionDuring the peripartum, women undergo significant hormonal changes that are crucial for fetal development and a healthy pregnancy and postpartum period for mother and infant. Although several studies have determined healthy norm ranges of estradiol and progesterone, there are discrepancies among the reports, rendering it unclear which hormone levels are linked to adverse health outcomes. To account for the impact of sex steroid patterns on health outcomes in mothers and children, a longitudinal assessment of different parameters is needed.Materials and methodsWe longitudinally assessed a cohort of 130 women over five months during pregnancy and postpartum. The women provided saliva samples and completed psychosocial questionnaires. Hormone analyses were conducted using enzyme-linked immunosorbent assay (ELISA). Different parameters of estradiol and progesterone were analyzed and evaluated in relation to psychometric variables. To examine the presence of heterogenous hormonal trajectories in the peripartum, we applied group-based trajectory modelling as a special case of latent-class group analysis.ResultsEstradiol and progesterone levels rose towards the end of pregnancy and dropped sharply after birth, with considerable individual variation, particularly during pregnancy. However, their ratio remained stable. We identified three estradiol trajectory subgroups and two progesterone subgroups. Age influenced progesterone levels, with older pregnant women having higher levels than younger women. Anxiety and depressive symptoms had a predictive value for trajectories of specific subgroups of women. The study also revealed two distinct subgroups regarding the course of estradiol and progesterone fluctuations as well as their ratio.ConclusionThis study provides insights into the course and fluctuation of salivary estradiol and progesterone levels among healthy women during the peripartum period, highlighting significant variations in hormone levels but stability in their ratio during this time. The finding of distinct sex steroid courses in the peripartum is new and suggests the need for further research to explore their impact on health outcomes. Our preliminary results suggest that hormonal fluctuations at the end of pregnancy appear to be a normal occurrence and might even be a protective factor for associated psychological symptoms and sleep disturbances in women.