bluuebunny/crossref_metadata_2025_split huggingface dataset:
POTENCY OF RHIZOSPHERE BACTERIA TO PROMOTE RICE GROWTH UNDER SALINE CONDITION
Authors: Widawati Sri , Sudiana I Made 
Published: October 2017

Saline soil is a common problem in coastal paddy field, especially in Indonesia. Salinity affects rice growth and the activities of soil functional microbes, including functional bacteria, which play roles in plant growth. Some of these microbes are associated with rice plants and are able to survive under saline condition. The presence of functional microbes is also important to improve soil quality. Nitrogen and phosphate are essential soil nutrients and is available in soil due to the activities of nitrogen-fixing bacteria and free-living plant-associated bacteria.Â The objective of the present study was to obtain nitrogen-fixing, phosphate solubilizing and Indole Acetic Acid Â (IAA)-producing bacteria that are able to survive and promote the growth of rice under saline conditions. From rice and peanut rhizosphere, Ca-phosphate (Ca-P) solubilizing and nitrogen-fixing bacteria were isolated separately using specific media. Then, the Ca-P solubilizing ability, phosphomonoesterase activity and IAA-producing ability were quantitatively examined. Based on the abilities, 20 strains were selected and identified as Burkholderia cepacia-complex, Burkholderia anthina, Burkholderia cenocepacia, Bacillus cereus-complex (three strains), Achromobacter spanius, Azospirillum sp. (four strains), Azotobacter sp. (three strains), Rhizobium leguminosarum, Rhizobium sp. (two strains), and Pseudomonas sp. (three strains). The inoculation of several single strains or the mixture of the selected strains promoted the growth of rice under saline conditions. These inoculants could be potential as biofertilizer in saline paddy fields.
POLLINATOR DIVERSITY AND FORAGING DYNAMICS ON MONSOON CROP OF CUCURBITS IN A TRADITIONAL LANDSCAPE OF SOUTH INDIAN WEST COAST
Authors: Balachandran C. , Chandran M. D. Subash , Vinay S. , Shrikant Naik , Ranachandra T. V. 
Published: October 2017

Studies on insect pollinator ecology and dynamics are very rarely carried out in traditional Indian agriculture landscapes. Indiscriminate landscape changes in the rural areas and tendencies towards crop monocultures can have significant effects on pollinator habitats and effectiveness. This study was aimed at observing insect pollinators, their visitation frequencies and timings on monsoon cucurbit crops such as Cucumis sativus L., C. pubescens Willd., Momordica charantia L., Trichonsanthes anguina L. and Luffa acutangula L. (Roxb.), in a coastal Karnataka Village. Â This study was also aimed at covering the significance of the surrounding landscape elements in sustaining pollinator elements.Â Bees, such as Apis dorsata, A. cerana and Trigona sp., were major visitors on all cucurbits, except snake gourd which was pollinated mainly by lepidopterans. Insect species were found to partition floral resources of any given crops between them by minimal overlapping in their visitation timings. Natural elements of the landscape around, mainly a village forest and rocky savanna furnished habitats for bees and lepidopterans. Prolifically blooming monsoon herbs on lateritic plateaus, by providing nectar resources for pollinators, presumably play key role in making the case study village well known for monsoon vegetables.
IDENTIFICATION OF SECRETORY STRUCTURE, HISTOCHEMISTRY AND PHYTOCHEMICAL COMPOUNDS OF MEDICINAL PLANT Hyptis capitata Jacq.
Authors: Rupa Darius , Sulistyaningsih Yohana Caecilia , Dorly Dorly , Ratnadewi Diah 
Published: October 2017

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves. Â The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing Â alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves Â contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
OPTIMIZATION AND EFFICIENCY IMPROVEMENT OF INâ€HOUSE IMMUNOAFFINITY COLUMN KU-AF02 FOR AFLATOXIN DETECTION
Authors: Kooprasertying Phakpoom , Maneeboon Thanapoom , Iamtaweejaroen Panrapee , Hongprayoon Ratchanee , Mahakarnchanakul Warapa 
Published: October 2017

In-house immunoaffinity column for aflatoxins (AFs) detection is firstly developed by Kasetsart University since 2011 i.e. the first IAC prototype, KU-AF01.Â Detection of aflatoxin B1 (AFB1) up to 200 ng is considered significantly efficient, but the production cost is considered high. The high cost was due to the cost of monoclonal antibody specific to aflatoxin and its supporting materials i.e. CNBr-activated sepharose 4B.Â Therefore, this study was aimed to improve the efficiency of in-house immunoaffinity colums by replacing CNBr-activated sepharose 4B with other supporting materials and to lower the ratio of antibody to supporting materials.Â Â Response surface methodology (RSM) was applied to determine the optimum alternative supporting materials and the ratio.Â Results revealed that when compared with CNBr-activated sepharose 4B, all materials tested could recover higher than 80% AFB1, except the activated CH sepharose 4B.Â Results also indicated that the optimum ratio was 0.4 mg anti-aflatoxin monoclonal antibody to 204 ÂµL CNBr-activated sepharose 4B.Â CNBr-activated sepharose 4B was considered to provide the best precision in recovering AFB1.Â KU-AF02 increased the ability to detect AFB1 to 500 ppb.Â The recovery of AFs in the reference materials using KU-AF02 was more than 96% successful, with HorRat value range of 0.34 â€“ 0.76.Â The reference materials used were 2 levels of AFs in peanuts, i.e. 47.68 and 72.14 ng/g.Â In terms of quality control in IAC production, there were no significant differences among the 5 batches produced (p > 0.05).Â KU-AF02 demonstrated stable and constant percent recovery at 4 oC for up to 12 months (tested with standard 200 ppb AFB1).Â This study indicated that KU-AF02 could lower the ratio of supporting materials to the antibody and that KU-AF02 has high stability and has good ability for cleaning up AFs.Â Therefore, KU-AF02 is recommended to be used as an in-house immunoaffinity column for aflatoxin detection in Thailand.
AN SNP MARKER POTENTIALLY LINKED TO SOMATIC EMBRYOGENESIS OF OIL PALM (Elaeis guineensis)
Authors: Wahyunanto Irang , Waturangi Diana E , Toruan-Mathius Nurita , Yulandi Adi 
Published: October 2017

Oil palm (Elaeis guineensis) is one of the most important oil-bearing crop in the world. This crop can be vegetatively propagated only using tissue culture technique. Oil palm tissue culture technique has low efficiency, with callogenesis and embryogenesis stages as the limiting factors. Genetic factor has a major role in determining the success rate of these two stages. The use of molecular markers which represent the rate of embryogenesis or callogenesis has the potential to improve the efficiency of oil palm tissue culture process. In this study, SNP mining was conducted on embryogenesis transcriptome data, oil palm cDNA database, oil palm genome database, and oil palm SNPs marker database in NCBI. Â The objective of this study was Â to obtain SNP marker which represents the embryogenesis potential, to be further used in marker assisted selection of oil palm ortets. One SNP (EMB6) showed significant association with embryogenesis rate. This SNP was found in one of Auxin Response Factor (ARF) family gene. Nucleotide replacement from Adenine to Guanine changed the 307th amino acid from Isoleucine to Methionine. Oil palms with Adenine homozygote (A/A) pattern on the EMB6 showed 8-fold higher chance to produce significantly higher embryogenesis rate than Adenine-Guanine heterozygote (A/G).
ANTIBACTERIAL ACTIVITY OF RED PIGMENT ISOLATED FROM COASTAL ENDOPHYTIC FUNGI AGAINST MULTI-DRUG RESISTANT BACTERIA
Authors: Sibero Mada Triandala , Sahara Rita , Syafiqoh Nur , Tarman Kustiariyah 
Published: October 2017

Multidrug-resistant (MDR) bacteria infections become a serious problem for these several decades. To solve this issue, finding of new antibiotics candidate in an urgency. Natural pigment is known to has biological activity against pathogenic bacteria. Coastal fungi are unexplored source of natural pigment to fight MDR bacteria. This research was aimed to isolate coastal endophytic fungi from smooth ant plant (Hydophytum formicarum), to screen endophytic fungi which produce red pigment, to extract the red pigment, to determine antibacterial activity of the red pigment and to identify the coastal endophytic fungi producing the red pigment. In this study, 7 fungi were isolated as endophytic fungi from H. formicarum. There were 3 isolates which produced extracellular pigment i.e. RS 1A which produced red pigment, RS 3 produced black pigment and RS 6A produced yellow pigment.Â Our study focused on red pigment which is produced by endophytic fungus strain RS 1A. The yield of red pigment was 8.8657% (w/w).Â This study showed that red pigment had antibacterial activity against Escherichia coli, Acinetobacter baumannii and Proteus mirabilis strain MDR. Judging from molecular and morphological identification, the endophytic fungus strain RS 1A was identified as Aspergillus versicolor.
МОДЕЛЮВАННЯ КЛІНІЧНИХ СИТУАЦІЙ – СТАНДАРТ ПРОГРАМИ СИМУЛЯЦІЙНОГО НАВЧАННЯ ЛІКАРІВ НА КАФЕДРІ НЕОНАТОЛОГІІ
Authors: Klymenko T. M. , Sandulyak T. V. , Serdtseva O. A. , Karapetyan O. Y. , Zakrevskyi A. M. 
Published: October 2017

Мета роботи – підвищити ефективність навчання лікарів за програмою первинної реанімації новонароджених шляхом моделювання клінічних ситуацій при набутті компетентності спеціалістів закладу родопомочі працювати в командах, акцентувати увагу на важливості симуляційного навчання дорослих для оволодіння алгоритмами надання дихальної підтримки та реанімаційної допомоги новонародженим в пологовій залі.Основна частина. У статті розглядаються сучасні принципи та методика симуляційного навчання лікарів шляхом моделювання клінічних ситуацій та відповідне матеріальне і методичне забезпечення виконання практичних навиків первинної реанімації новонароджених згідно з вимогами LICOR 2015 р.Проведені анкетування та оцінка результатів пре-тесту 200 слухачів та аналіз літературних джерел і власного досвіду роботи за методикою симуляційного навчання з використанням контрольних листів виконання практичних навичок і відеоспостереження.Методика симуляційного навчання значно розширила можливості навчального процесу та показала її ефективність у перинатальних центрах, але в пологових будинках регіону спеціалісти ще не охоплені необхідним рівнем навчання і показують гірші результати роботи по забезпеченню дихальної підтримки та реанімації новонароджених.На кафедрі неонатології ХМАПО проведена оцінка причин недостатньої ефективності навчання та роботи спеціалістів закладів родопомочі та змінена методика навчання. Використано педагогічне проектування кожної теми з моделювання клінічних ситуацій при симуляційному навчанні та набутті лікарями компетентності надання дихальної підтримки та реанімаційної допомоги, особливо недоношеним дітям, за алгоритмами LICOR 2015 року. Висновки. Досвід застосування моделювання клінічних ситуацій при симуляційному навчанні спеціалістів безпосередньо в закладах родопомочі дозволив підвищити ефективність навчального процесу і покращити показники роботи пологових будинків. Слухачі мають змогу отримати не лише теоретичну підготовку, а й в обов’язковому порядку оволодіти необхідними практичними навичками і вмінням роботи, що сприяє поліпшенню розуміння та засвоєння матеріалу, набуття професійної компетентності виконати вимоги LICOR 2015 року та протоколу МОЗ України Початкова, реанімаційна і післяреанімаційна допомога новонародженим в Україні 2014 року.
Hamstring Graft Incorporation According to the Length of the Graft Inside Tunnels
Authors: Cavaignac Etienne , Marot Vincent , Faruch Marie , Reina Nicolas , Murgier Jérôme , Accadbled Franck , Berard Emilie , Chiron Philippe 
Published: October 2017

Background: Anterior cruciate ligament (ACL) reconstruction with a quadrupled semitendinosus (ST4) graft is an evolution of the standard technique with 2 hamstring tendons (semitendinosus + gracilis [STG]). However, there is no published comparison of how well these 2 types of hamstring grafts are incorporated into the bone tunnels. Because the ST4 graft is shorter, there is less graft material inside the tunnels. Purpose: To use magnetic resonance imaging (MRI) to compare graft incorporation in the tibial bone tunnels 1 year after ACL reconstruction with either an STG graft or ST4 graft. Study Design: Cohort study; Level of evidence, 2. Methods: Sixty-two patients who underwent ACL reconstruction were enrolled prospectively: 31 with an ST4 graft and 31 with an STG graft. The same surgical technique, fixation method, and postoperative protocol were used in both groups. Graft incorporation and ligamentization were evaluated with MRI after 1 year of follow-up. The following parameters were evaluated: signal-to-noise quotient (SNQ), tibial tunnel enlargement, signal intensity at the bone-graft interface, and graft signal according to the Howell scale. The number of participants needed to show that the mean SNQ did not differ between the 2 techniques was 31 in each group (with a 1-sided alpha of 2.5% and a 1-sided beta of 10.0%). The Student t test was used to compare the distribution of continuous secondary endpoints. Results: The mean SNQ was 5.2 ± 4.5 for the STG group and 5.9 ± 3.7 for the ST4 group ( P = .5100). The mean tibial tunnel widening was 93.7% ± 51.7% for the STG group versus 80.0% ± 42.9% for the ST4 group ( P = .2605). The groups did not differ in signal intensity at the bone-graft interface ( P = .7502) or in graft signal according to the Howell scale ( P = .4544). Conclusion: At the 1-year postoperative follow-up, incorporation and ligamentization of the STG and ST4 grafts were the same based on MRI analysis. The results were at least as good with the ST4 technique as with the standard STG technique in terms of incorporation and ligamentization.
Incident adverse drug reactions in geriatric inpatients: a multicentred observational study
Authors: Lavan Amanda , Eustace Joseph , Dahly Darren , Flanagan Evelyn , Gallagher Paul , Cullinane Shane , Petrovic Mirko , Perehudoff Katrina , Gudmondsson Adalsteinn , Samuelsson Ólafur , Sverrisdóttir Ástrós , Cherubin Antonio , Dimitri Frederica , Rimland Joe , Cruz-Jentoft Alfonso , Vélez-Díaz-Pallarés Manuel , Lozano Montoya Isabel , Soiza Roy L. , Subbarayan Selvarani , O’Mahony Denis 
Published: October 2017

Background: Adverse drug reactions (ADRs) are common in older adults and frequently have serious clinical and economic consequences. This study was conducted as a feasibility study for a randomized control trial (RCT) that will investigate the efficacy of a software engine to optimize medications and reduce incident (in-hospital) ADRs. This study’s objectives were to (i) establish current incident ADR rates across the six sites participating in the forthcoming RCT and (ii) assess whether incident ADRs are predictable. Methods: This was a multicentre, prospective observational study involving six European hospitals. Adults aged ⩾ 65 years, hospitalized with an acute illness and on pharmacological treatment for three or more conditions were eligible for inclusion. Adverse events (AEs) were captured using a trigger list of 12 common ADRs. An AE was deemed an ADR when its association with an administered drug was adjudicated as being probable/certain, according to the World Health Organization Uppsala Monitoring Centre causality assessment. The proportion of patients experiencing at least one, probable/certain, incident ADR within 14 days of enrolment/discharge was recorded. Results: A total of 644 patients were recruited, evenly split by sex and overwhelmingly of White ethnicity. Over 80% of admissions were medical. The median number of chronic conditions was five (interquartile range 4–6), with eight or more conditions present in approximately 10%. The mean number of prescribed medications was 9.9 (standard deviation 3.8), which correlated strongly with the number of conditions ( r = 0.54, p < 0.0001). A total of 732 AEs were recorded in 382 patients, of which 363 were incident. The majority of events were classified as probably or possibly drug related, with heterogeneity across sites (χ2 = 88.567, df = 20, p value < 0.001). Out of 644 patients, 139 (21.6%; 95% confidence interval 18.5–25.0%) experienced an ADR. Serum electrolyte abnormalities were the most common ADR. The ADRROP (ADR Risk in Older People) and GerontoNet ADR risk scales correctly predicted ADR occurrence in 61% and 60% of patients, respectively. Conclusion: This feasibility study established the rates of incident ADRs across the six study sites. The ADR predictive power of ADRROP and GerontoNet ADR risk scales were limited in this population.
The Role of Lateral Extra-articular Tenodesis in Primary Anterior Cruciate Ligament Reconstruction: A Systematic Review With Meta-analysis and Best-Evidence Synthesis
Authors: Devitt Brian M. , Bell Stuart W. , Ardern Clare L. , Hartwig Taylor , Porter Tabitha J. , Feller Julian A. , Webster Kate E. 
Published: October 2017

Background: The role of lateral extra-articular tenodesis (LEAT) to augment primary anterior cruciate ligament reconstruction (ACLR) remains controversial. Purpose: To determine whether the addition of LEAT to primary ACLR provides greater control of rotational laxity and improves clinical outcomes compared with ACLR alone and to assess the impact of early versus delayed ACLR. Study Design: Systematic review; Level of evidence, 3. Methods: Two reviewers independently searched 7 databases for randomized and nonrandomized clinical studies comparing ACLR plus LEAT versus ACLR alone. Animal, cadaveric, and biomechanical studies; revision or repair procedures; and studies using synthetic ligaments and multiligamentous-injured knees were excluded. Risk of bias was assessed with a modified Downs and Black checklist. The primary outcome was postoperative pivot shift. These data were pooled by use of a fixed-effects meta-analysis model. The studies were divided into delayed (>12 months) and early (≤12 months) reconstruction groups for meta-analysis. A best-evidence synthesis was performed on the remaining outcome measures. Results: Of 387 titles identified, 11 articles were included (5 of high quality). Meta-analysis of postoperative pivot shift in 3 studies of delayed primary ACLR showed a statistically significant difference for the pivot-shift test in favor of ACLR with LEAT (odds ratio [OR], 0.44; 95% confidence interval [CI], 0.24-0.81; P = .008; I2 = 0). Meta-analysis of 5 studies of early primary ACLR found no statistically significant difference with the addition of LEAT (OR, 0.60; 95% CI, 0.33-1.09; P = .10; I2 = 33%). Insufficient evidence was available to determine whether the addition of LEAT had any effect on clinical, objective, subjective, and functional outcomes. Conclusion: In primary ACLR, no evidence is available showing additional benefit of LEAT in reducing the postoperative pivot shift in early reconstructions (≤12 months); however, LEAT may have a role in delayed ACLR. Strong evidence exists that a combined ACLR and LEAT reduces lateral femoral translation, but there is insufficient evidence to identify any benefit for other clinical outcomes.
Prediction models for clinical outcome after a carotid revascularisation procedure: A systematic review
Authors: Volkers Eline J , Algra Ale , Kappelle L Jaap , Greving Jacoba P 
Published: October 2017

Introduction Prediction models for clinical outcome after carotid artery stenting or carotid endarterectomy could aid physicians in estimating peri- and postprocedural risks in individual patients. We aimed to identify existing prediction models for short- and long-term outcome after carotid artery stenting or carotid endarterectomy in patients with symptomatic or asymptomatic carotid stenosis, and to summarise their most important predictors and predictive performance. Patients and methods We performed a systematic literature search for studies that developed a prediction model or risk score published until 22 December 2016. Eligible prediction models had to predict the risk of vascular events with at least one patient characteristic. Results We identified 37 studies that developed 46 prediction models. Thirty-four (74%) models were developed in carotid endarterectomy patients; 27 of these (59%) predicted short-term (in-hospital or within 30 days) risk. Most commonly predicted outcome was stroke or death (n = 12; 26%). Age (n = 31; 67%), diabetes mellitus (n = 21; 46%), heart failure (n = 16; 35%), and contralateral carotid stenosis ≥50% or occlusion (n = 16; 35%) were most commonly used as predictors. For 25 models (54%), it was unclear how missing data were handled; a complete case analysis was performed in 15 (33%) of the remaining 21 models. Twenty-eight (61%) models reported the full regression formula or risk score with risk classification. Twenty-one (46%) models were validated internally and 12 (26%) externally. Discriminative performance (c-statistic) ranged from 0.66 to 0.94 for models after carotid artery stenting and from 0.58 to 0.74 for models after carotid endarterectomy. The c-statistic ranged from 0.55 to 0.72 for the external validations. Discussion Age, diabetes mellitus, heart failure, and contralateral carotid stenosis ≥50% or occlusion were most often used as predictors in all models. Discriminative performance (c-statistic) was higher for prediction models after carotid artery stenting than after carotid endarterectomy. Conclusion The clinical usefulness of most prediction models for short- or long-term outcome after carotid artery stenting or carotid endarterectomy remains unclear because of incomplete reporting, methodological limitations, and lack of external validation.