bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immobilization of single argon atoms in nano-cages of two-dimensional zeolite model systems
Authors: Zhong Jian-Qiang , Wang Mengen , Akter Nusnin , Kestell John D. , Boscoboinik Alejandro M. , Kim Taejin , Stacchiola Dario J. , Lu Deyu , Boscoboinik J. Anibal 
Published: July 2017

AbstractThe confinement of noble gases on nanostructured surfaces, in contrast to bulk materials, at non-cryogenic temperatures represents a formidable challenge. In this work, individual Ar atoms are trapped at 300 K in nano-cages consisting of (alumino)silicate hexagonal prisms forming a two-dimensional array on a planar surface. The trapping of Ar atoms is detected in situ using synchrotron-based ambient pressure X-ray photoelectron spectroscopy. The atoms remain in the cages upon heating to 400 K. The trapping and release of Ar is studied combining surface science methods and density functional theory calculations. While the frameworks stay intact with the inclusion of Ar atoms, the permeability of gasses (for example, CO) through them is significantly affected, making these structures also interesting candidates for tunable atomic and molecular sieves. These findings enable the study of individually confined noble gas atoms using surface science methods, opening up new opportunities for fundamental research.
Tid1-S regulates the mitochondrial localization of EGFR in non-small cell lung carcinoma
Authors: Wang T-H , Lin Y-H , Yang S-C , Chang P-C , Wang T-CV , Chen C-Y 
Published: July 2017

AbstractThe epidermal growth factor receptor (EGFR) is the major driver of non-small cell lung carcinoma (NSCLC). Mitochondrial accumulation of EGFR has been shown to promote metastasis in NSCLC, yet little is known about how the mitochondrial localization of EGFR is regulated. In this work, we show that Tid1 (also known as mitochondrial HSP40) is involved in the mitochondrial localization of EGFR, and that the DnaJ domain of Tid1-S is essential for the Tid1-S-mediated transportation of EGFR into mitochondria. Overexpression of Tid1-S increased the migration and invasion of NSCLC cells cultured in vitro. High levels of EGFR and Tid1-S were detected in the mitochondria of cancerous lesions from stage IV NSCLC patients, and high levels of mitochondrial Tid1-S/EGFR were correlated with lymph node metastasis and poor overall survival of NSCLC patients. We thus conclude that Tid1-S critically governs the mitochondrial localization of EGFR through the mtHSP70 transportation pathway, and that the mitochondrial accumulation of EGFR appears to promote metastasis in NSCLC.
Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
Authors: Cerrone Marina , Montnach Jerome , Lin Xianming , Zhao Yan-Ting , Zhang Mingliang , Agullo-Pascual Esperanza , Leo-Macias Alejandra , Alvarado Francisco J. , Dolgalev Igor , Karathanos Thomas V. , Malkani Kabir , Van Opbergen Chantal J.M. , van Bavel Joanne J.A. , Yang Hua-Qian , Vasquez Carolina , Tester David , Fowler Steven , Liang Fengxia , Rothenberg Eli , Heguy Adriana , Morley Gregory E. , Coetzee William A. , Trayanova Natalia A. , Ackerman Michael J. , van Veen Toon A.B. , Valdivia Hector H. , Delmar Mario 
Published: July 2017

AbstractPlakophilin-2 (PKP2) is a component of the desmosome and known for its role in cell–cell adhesion. Mutations in human PKP2 associate with a life-threatening arrhythmogenic cardiomyopathy, often of right ventricular predominance. Here, we use a range of state-of-the-art methods and a cardiomyocyte-specific, tamoxifen-activated, PKP2 knockout mouse to demonstrate that in addition to its role in cell adhesion, PKP2 is necessary to maintain transcription of genes that control intracellular calcium cycling. Lack of PKP2 reduces expression of Ryr2 (coding for Ryanodine Receptor 2), Ank2 (coding for Ankyrin-B), Cacna1c (coding for CaV1.2) and Trdn (coding for triadin), and protein levels of calsequestrin-2 (Casq2). These factors combined lead to disruption of intracellular calcium homeostasis and isoproterenol-induced arrhythmias that are prevented by flecainide treatment. We propose a previously unrecognized arrhythmogenic mechanism related to PKP2 expression and suggest that mutations in PKP2 in humans may cause life-threatening arrhythmias even in the absence of structural disease.
Synthesis of ordered carbonaceous frameworks from organic crystals
Authors: Nishihara Hirotomo , Hirota Tetsuya , Matsuura Kenta , Ohwada Mao , Hoshino Norihisa , Akutagawa Tomoyuki , Higuchi Takeshi , Jinnai Hiroshi , Koseki Yoshitaka , Kasai Hitoshi , Matsuo Yoshiaki , Maruyama Jun , Hayasaka Yuichiro , Konaka Hisashi , Yamada Yasuhiro , Yamaguchi Shingi , Kamiya Kazuhide , Kamimura Takuya , Nobukuni Hirofumi , Tani Fumito 
Published: July 2017

AbstractDespite recent advances in the carbonization of organic crystalline solids like metal-organic frameworks or supramolecular frameworks, it has been challenging to convert crystalline organic solids into ordered carbonaceous frameworks. Herein, we report a route to attaining such ordered frameworks via the carbonization of an organic crystal of a Ni-containing cyclic porphyrin dimer (Ni2-CPDPy). This dimer comprises two Ni–porphyrins linked by two butadiyne (diacetylene) moieties through phenyl groups. The Ni2-CPDPy crystal is thermally converted into a crystalline covalent-organic framework at 581 K and is further converted into ordered carbonaceous frameworks equipped with electrical conductivity by subsequent carbonization at 873–1073 K. In addition, the porphyrin’s Ni–N4 unit is also well retained and embedded in the final framework. The resulting ordered carbonaceous frameworks exhibit an intermediate structure, between organic-based frameworks and carbon materials, with advantageous electrocatalysis. This principle enables the chemical molecular-level structural design of three-dimensional carbonaceous frameworks.
DNA end resection requires constitutive sumoylation of CtIP by CBX4
Authors: Soria-Bretones Isabel , Cepeda-García Cristina , Checa-Rodriguez Cintia , Heyer Vincent , Reina-San-Martin Bernardo , Soutoglou Evi , Huertas Pablo 
Published: July 2017

AbstractDNA breaks are complex DNA lesions that can be repaired by two alternative mechanisms: non-homologous end-joining and homologous recombination. The decision between them depends on the activation of the DNA resection machinery, which blocks non-homologous end-joining and stimulates recombination. On the other hand, post-translational modifications play a critical role in DNA repair. We have found that the SUMO E3 ligase CBX4 controls resection through the key factor CtIP. Indeed, CBX4 depletion impairs CtIP constitutive sumoylation and DNA end processing. Importantly, mutating lysine 896 in CtIP recapitulates the CBX4-depletion phenotype, blocks homologous recombination and increases genomic instability. Artificial fusion of CtIP and SUMO suppresses the effects of both the non-sumoylatable CtIP mutant and CBX4 depletion. Mechanistically, CtIP sumoylation is essential for its recruitment to damaged DNA. In summary, sumoylation of CtIP at lysine 896 defines a subpopulation of the protein that is involved in DNA resection and recombination.
Robust hierarchical 3D carbon foam electrode for efficient water electrolysis
Authors: Pham Tung Ngoc , Sharifi Tiva , Sandström Robin , Siljebo William , Shchukarev Andrey , Kordas Krisztian , Wågberg Thomas , Mikkola Jyri-Pekka 
Published: July 2017

AbstractHerein we report a 3D heterostructure comprising a hierarchical macroporous carbon foam that incorporates mesoporous carbon nanotubes decorated with cobalt oxide nanoparticles as an unique and highly efficient electrode material for the oxygen evolution reaction (OER) in electrocatalytic water splitting. The best performing electrode material showed high stability after 10 h, at constant potential of 1.7 V vs. RHE (reversible hydrogen electrode) in a 0.1 M KOH solution and high electrocatalytic activity in OER with low overpotential (0.38 V vs RHE at 10 mA cm−2). The excellent electrocatalytic performance of the electrode is rationalized by the overall 3D macroporous structure and with the firmly integrated CNTs directly grown on the foam, resulting in a large specific surface area, good electrical conductivity, as well as an efficient electrolyte transport into the whole electrode matrix concurrent with an ability to quickly dispose oxygen bubbles into the electrolyte. The eminent properties of the three-dimensional structured carbon matrix, which can be synthesized through a simple, scalable and cost effective pyrolysis process show that it has potential to be implemented in large-scale water electrolysis systems.
How cholesteryl ester transfer protein can also be a potential triglyceride transporter
Authors: Chirasani Venkat R. , Senapati Sanjib 
Published: July 2017

AbstractCETP transfers cholesteryl esters (CEs) and triglycerides (TGs) between different lipoproteins and came in limelight as a drug-target against CVD. In the search for detailed mechanism of lipid transfer through CETP, enormous effort is devoted employing crystallographic, cryo-EM, and Molecular Dynamics (MD) studies. However, these studies primarily focused on CE-bound CETP structure and CE transfer mechanism. With the reported correlation that CETP looses significant CE transfer activity upon inhibiting TG transfer, it is of tremendous importance to understand the structure and dynamics of TG-bound CETP. Our results from large-scale all-atom and coarse-grained MD simulations show that CETP can accommodate two TG molecules in parallel N-N orientation with TG oleate chains majorly attaining the tuning-fork conformation. In TG-bound form, CETP not only maintained its secondary structures but also exhibited similar bending-twisting motions as reported for CE-CETP crystal structure. Obtained structural information are further validated by correlating to available functional data of 2–8 fold slower transfer rate of TG through CETP, where we show that TGs make 20% additional contacts with CETP compared to CEs. Identified CETP residues facilitating TG binding also match very well with reported mutagenesis data. The study could accelerate the drug-designing processes to combat CETP functionality and CVD.
Improved Liver R2* Mapping by Averaging Decay Curves
Authors: Zhang Xinyuan , Peng Jie , Wang Changqing , Feng Yanqiu , Feng Qianjin , Li Xinzhong , Chen Wufan , He Taigang 
Published: July 2017

AbstractLiver R2* mapping is often degraded by the low signal-to-noise ratio (SNR) especially in the presence of severe iron. This study aims to improve liver R2* mapping at low SNRs by averaging decay curves before the process of curve-fitting. Independently filtering echo images by nonlocal means (NLM) demonstrated improved quality of R2* mapping, but may introduce new errors due to the nonlinear nature of the NLM filter, during which the averaging weights may vary with different image contents at multiple echo times. In addition, the image denoising effect of the NLM may decline when no sufficient similar patches are available. To overcome these drawbacks, we proposed to filter decay curves instead of images. In this novel scheme, decay curves were averaged in a local window, each with a weight assigned according to the curve-similarity measured by the distance between one of the neighboring curves and the targeted one. The proposed method was tested on simulated, phantom and patient data. The results demonstrate that the proposed method can provide more accurate R2* mapping compared with the NLM algorithm, and hence has the potential to improve diagnosis and therapy in patients with liver iron.
Thickness dependence of anomalous Nernst coefficient and longitudinal spin Seebeck effect in ferromagnetic NixFe100−x films
Authors: Kannan Harsha , Fan Xin , Celik Halise , Han Xiufeng , Xiao John Q. 
Published: July 2017

AbstractSpin Seebeck effect (SSE) measured for metallic ferromagnetic thin films in commonly used longitudinal configuration contains the contribution from anomalous Nernst effect (ANE). The ANE is considered to arise from the bulk of the ferromagnet (FM) and the proximity-induced FM boundary layer. We fabricate a FM alloy with zero Nernst coefficient to mitigate the ANE contamination of SSE and insert a thin layer of Cu to separate the heavy metal (HM) from the FM to avoid the proximity contribution. These modifications to the experiment should permit complete isolation of SSE from ANE in the longitudinal configuration. However, further thickness dependence studies and careful analysis of the results revealed, ANE contribution of the isolated FM alloy is twofold, surface and bulk. Both surface and bulk contributions, whose magnitudes are comparable to that of the SSE, can be modified by the neighboring layer. Hence surface contribution to the ANE in FM metals is an important effect that needs to be considered.
Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix
Authors: Bodenberger Nicholas , Kubiczek Dennis , Trösch Laura , Gawanbacht Ali , Wilhelm Susanne , Tielker Denis , Rosenau Frank 
Published: July 2017

Abstract3D cell culture is a helpful approach to study cell-cell interaction in a native-like environment, but is often limited due the challenge of retrieving cells from the material. In this study, we present the use of recombinant lectin B, a sugar-binding protein with four binding cavities, to enable reversible cell integration into a macroporous protein hydrogel matrix. By functionalizing hydrogel precursors with saccharose, lectin B can both bind to sugar moieties on the cellular surface as well as to the modified hydrogel network. Confocal microscopy and flow cytometry analysis revealed cells to be integrated into the network and to adhere and proliferate. Furthermore, the specificity and reversibility was investigated by using a recombinantly produced yellow fluorescent - lectin B fusion protein and a variety of sugars with diverging affinities for lectin B at different concentrations and elution times. Cells could be eluted within minutes by addition of L-fucose to the cell-loaded hydrogels to make cells available for further analysis.
Role of HDAC9-FoxO1 Axis in the Transcriptional Program Associated with Hepatic Gluconeogenesis
Authors: Chen Jizheng , Zhang Zhilei , Wang Ning , Guo Min , Chi Xiumei , Pan Yu , Jiang Jing , Niu Junqi , Ksimu Sulaiman , Li John Zhong , Chen Xinwen , Wang Qian 
Published: July 2017

AbstractHistone deacetylase 9 (HDAC9) regulates hepatic gluconeogenesis by deacetylating Forkhead box O 1 (FoxO1). HDAC9 upregulation is involved in hepatitis C virus (HCV)-associated exaggerated gluconeogenesis. Herein, we found in addition to FoxO1, HDAC9 also regulates other gluconeogenic transcription factors, including peroxisomeproliferator-activated receptor-γ coactivator-1α (PGC-1α), cyclic AMP-responsive element-binding protein (CREB), and glucocorticoid receptor (GR). Unlike FoxO1, which is regulated by post-translational modification responses to HDAC9, HDAC9 regulates PGC-1α, CREB and GR by altering gene expression. Similar to PGC-1α, CREB and GR were found to be novel regulatory targets of FoxO1 by examination of the FoxO1 binding site in their promoter. PGC-1α, CREB and GR were upregulated in response to HDAC9 via FoxO1 deacetylation. These findings indicate that HDAC9-FoxO1 signalling contributes to gluconeogenesis by modulating the expression of gluconeogenic transcription factors. In particular, metabolic profiling demonstrated a clear shift towards gluconeogenesis metabolism, and HDAC9-FoxO1 signalling can be strongly induced to upregulate gluconeogenic transcription factors following HCV infection. The positive correlation between HDAC9 and gluconeogenic transcription factor expression levels in the livers of both HCV-infected patients and normal individuals further emphasizes the clinical relevance of these results. Thus, HDAC9-FoxO1 signalling axis is involved in regulating gluconeogenic transcription factors, gluconeogenesis, and HCV-induced type 2 diabetes.
Partitioning Evapotranspiration into Green and Blue Water Sources in the Conterminous United States
Authors: Velpuri Naga Manohar , Senay Gabriel B. 
Published: July 2017

AbstractIn this study, we combined two 1 km actual evapotranspiration datasets (ET), one obtained from a root zone water balance model and another from an energy balance model, to partition annual ET into green (rainfall-based) and blue (surface water/groundwater) sources. Time series maps of green water ET (GWET) and blue water ET (BWET) are produced for the conterminous United States (CONUS) over 2001–2015. Our results indicate that average green and blue water for all land cover types in CONUS accounts for nearly 70% and 30% of the total ET, respectively. The ET in the eastern US arises mostly from GWET, and in the western US, it is mostly BWET. Analysis of the BWET in the 16 irrigated areas in CONUS revealed interesting results. While the magnitude of the BWET gradually showed a decline from west to east, the increase in coefficient of variation from west to east confirmed greater use of supplemental irrigation in the central and eastern US. We also established relationships between different hydro-climatology zones and their blue water requirements. This study provides insights on the relative contributions and the spatiotemporal dynamics of GWET and BWET, which could lead to improved water resources management.
CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex
Authors: Kostyukevich Yury , Shulga Aleksej A. , Kononikhin Alexey , Popov Igor , Nikolaev Eugene , Deyev Sergey 
Published: July 2017

AbstractThe barnase-barstar complex is one of the most stable protein-protein complexes and has a very wide range of possible applications. Here we report the use of top-down mass spectrometry for the investigation of the structure of this complex, its ionization via ESI, isolation and fragmentation. It was found that the asymmetry of the resulting charge state distributions of the protein monomer product ions increased as the charge state of the precursor ions increased. For the investigation of the 3D structure of the complex, the gas phase H/D exchange reaction was used. In addition, supermetallized ions of the complex with Zn were produced and investigated. It was observed that an increase in the number of metals bound to the complex results in a change in complex stability and the charge distribution between protein fragment. Analysis of the fragmentation pattern of the supermetallized complex [bn-b* + 5Zn]10+ indicated that this ion is present in different conformations with different charges and Zn distributions. Since Zn cannot migrate, such structures must be formed during ionization.
GeneXpert MTB/RIF assay in the diagnosis of urinary tuberculosis from urine specimens
Authors: Pang Yu , Shang Yuanyuan , Lu Jie , Liang Qian , Dong Lingling , Li Yunxu , Zhao Liping , Jiang Guanglu , Huang Hairong 
Published: July 2017

AbstractConventional bacteriological methods are not generally helpful in diagnosing urinary tuberculosis (UTB). GeneXpert is endorsed for the detection of pulmonary tuberculosis, whereas the data on its utility for urine specimens is limited. In this study, we aimed to evaluate its performance on urine specimens in a country with high TB incidence. A total of 163 suspected UTB patients were consecutively enrolled in the analysis, including 37 (22.7%) culture-positive and 44 (27.0%) clinically diagnosed UTB cases. Compared with conventional culture, the sensitivity of GeneXpert (94.6%) was significantly higher than that of smear microscopy (40.5%, P < 0.001). When setting clinical diagnosis as gold standard, 51 out of 81 clinically diagnosed UTB cases were detected by GeneXpert, demonstrating a sensitivity of 63.0%, which was significantly higher than that of smear microscopy (18.5%, P < 0.001) and culture (45.7%, P = 0.027), respectively. In addition, the proportion of UTB cases in the migrant population was significantly higher than that in the resident population (P = 0.019). To conclude, our data demonstrate that GeneXpert outperforms AFB smear and culture for the detection of MTB in urine samples, which provides an alternative for the diagnosis of UTB. The migrant population and previously diagnosed TB cases are high risk factors for developing UTB cases.
Efficacy of Cyclophosphamide treatment for immunoglobulin G4-related disease with addition of glucocorticoids
Authors: Yunyun Fei , Yu Chen , Panpan Zhang , Hua Chen , Di Wu , Lidan Zhao , Linyi Peng , Li Wang , Qingjun Wu , Xuan Zhang , Yan Zhao , Xiaofeng Zeng , Fengchun Zhang , Wen Zhang 
Published: July 2017

AbstractAim to evaluate the efficacy and safety of glucocorticoid monotherapy vs combination therapy of cyclophosphamide (CYC) for IgG4 related disease (IgG4-RD). 102 newly diagnosed IgG4-RD patients were enrolled and assigned to 2 groups: Group I was prednisone monotherapy (0.5–1.0 mg/kg.d, tapered gradually) and Group II was glucocorticoid and CYC (50–100 mg per day). Patients were assessed at different periods. Primary end point was relapse rate; secondary end points included response, remission rate and adverse effects. 52 patients were in Group I and 50 in Group II. At 1 month, both groups achieved obvious improvement. Accumulated relapse rate during 1 year was 38.5% in Group 1, including 12 cases with clinical relapse and 8 patients manifesting only serological relapse; whereas there was 12.0% of relapse in Group 2, only 1 with clinical relapse and other 5 patients got serological relapse. The mean flare time in Group II was significantly longer than that in Group I. All relapsing patients in Group I were sensitive to immunosuppressants. Most patients involving more than 6 organs in Group I relapsed during 1 year. IgG4 levels of relapse cases were significantly higher than non-relapsing patients at baseline. Bile duct, lacrimal glands and lymph nodes were commonly relapsed organs in Group I.
Photoluminescence associated with the site occupations of Ho3+ ions in BaTiO3
Authors: Lu Da-Yong , Guan Dong-Xue 
Published: July 2017

AbstractA nominal (Ba1−x
Ho
x
)Ti1−x/4O3 (x = 0.01) (BHTH) ceramic with a single-phase tetragonal structure was prepared at 1400 °C using the solid-state reaction method. The analysis on the defect chemistry revealed that the real formula of BHTH is (Ba1−x
Ho3x/4)(Ti1−x/4Ho
x/4)O3 with Ba vacancies via electron paramagnetic resonance (EPR). Photoluminescence (PL) was investigated on the basis of excitation with different wavelength lasers. The results indicated that under 488-nm excitation, PL and Raman scattering can occur simultaneously as two distinct optical processes for BHTH ceramic powders, and the strongest PL band at 564 nm was discovered and verified to originate from the 5G6/5F1 → 5I7 transition of Ho3+ ions on the Ti sites in the BaTiO3 lattice. Upon 532- and 638-nm excitations, three PL bands of 5F4/5S2 → 5I8, 5F5 → 5I8, and 5F4/5S2 → 5I7 transitions are attributed to the contributions from Ho3+ ions on the Ba sites. The common Raman spectrum of BaTiO3 can be observed without PL disturbance using 785-nm excitation wavelength. The PL effect may provide a probe for the site occupations of Ho3+ ions in widely-used BaTiO3 dielectric ceramics co-doped with Ho3+ and other dopants.