bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhizobium as a biofertilizer for non-leguminous plants
Authors: Pereira Flory , Pereira Aaron , Menezes Larissa Danielle , Sawaikar Ranjita 
Published: September 2024

AbstractUtilising biofertilizers is a sustainable alternative of integrated nutrient management in plants. Commercialized biofertilizers for non-legumes include Azotobacter and Phosphate Solubilizing Bacteria (PSB), without reported adverse effects on human health, including consumption of raw salad vegetables. However, no commercial biofertilizers for non-legumes are based on rhizobia, despite proven human safety from decades of legume inoculation research. A comparative analysis investigating the yield of tomatoes (Solanum lycopersicum), and content of Lycopene, Vitamin C and antioxidants was undertaken to assess the potential commercial viability of Rhizobium as a biofertilizer for non-leguminous plants. Rhizobium amendment gave a lesser yield of tomatoes (3 ± 1) compared to Azotobacter (4 ± 1), but was better than the control (2 ± 1), and PSB (2 ± 1). It showed highest lycopene content (0.7 ± 0.02 mg/100 g) compared to other amendments. The Vitamin C (32.4 ± 0.01 mg/100 g) with Rhizobium amendment surpassed that of Azotobacter (24.9 ± 0.09 mg/100 g) and control (12.84 ± 0.03 mg/100 g). Antioxidant activity of cherry tomatoes grown in Rhizobium-amended soil, was 1.34 times higher than chemically-fertilized plant yield. This study therefore affirms the suitability of rhizobia as a biofertilizer for stimulating the growth and productivity of non-leguminous plants such as tomato (S. lycopersicum) and its potential to be commercialized for use in non-leguminous plants.
clocks replaced with : ; . [Deschooling Time in the Small Isles]
Authors: Simpson Kate Elspeth 
Published: September 2024

Abstract
The Scottish Small Isles – comprising Muck, Rùm, Eigg, Canna, and by extension, Coll – are geologically complex, with intersecting rock samples from the Archean (Lewisian Gneiss basements formed approximately 3 billion years ago), Proterozoic (Torridonian sandstone formed approximately 1 billion years ago), Mesozoic (sedimentation deposited approximately 200 million years ago) and Palaeocene (basalt formed approximately 55.8 million years ago as part of the Palaeocene–Eocene Thermal Maximum event). This practice research article – drawing on palaeontology, kinaesthetic learning and creative writing – takes the Small Isles as a case study for what geologist Marcia Bjornerud defines as a discernible “timefulness” that humans should seek to attain: “an acute consciousness of how the world is made by–indeed, made of–time” (2020, Timefulness: How Thinking Like a Geologist Can Help Save the World, 5). Through their lithic intrusions, and interruptive strata, the Small Isles offer an alternative form of pedagogy: where multiple epochs, tenses and tempos visibly converse with one another; where “polytemporality” can be witnessed and physically experienced; where the notion of linear time is destabilised.
Molecular mechanism of prolactin-releasing peptide recognition and signaling via its G protein-coupled receptor
Authors: Li Yang , Yuan Qingning , He Xinheng , Zhang Yumu , You Chongzhao , Wu Canrong , Li Jingru , Xu H. Eric , Zhao Li-Hua 
Published: September 2024

AbstractProlactin-releasing peptide (PrRP) is an RF-amide neuropeptide that binds and activates its cognate G protein-coupled receptor, prolactin-releasing peptide receptor (PrRPR), also known as GPR10. PrRP and PrRPR are highly conserved across mammals and involved in regulating a range of physiological processes, including stress response, appetite regulation, pain modulation, cardiovascular function, and potentially reproductive functions. Here we present cryo-electron microscopy structures of PrRP-bound PrRPR coupled to Gq or Gi heterotrimer, unveiling distinct molecular determinants underlying the specific recognition of the ligand’s C-terminal RF-amide motif. We identify a conserved polar pocket that accommodates the C-terminal amide shared by RF-amide peptides. Structural comparison with neuropeptide Y receptors reveals both similarities and differences in engaging the essential RF/RY-amide motifs. Our findings demonstrate the general mechanism governing RF-amide motif recognition by PrRPR and RF-amide peptide receptors, and provide a foundation for elucidating activation mechanisms and developing selective drugs targeting this important peptide–receptor system.
Subgroup disproportionality analysis of dementia-related adverse events with sacubitril/valsartan across geographical regions
Authors: Kim Seong Kyung , Kim Myeong Gyu 
Published: September 2024

AbstractThis study aimed to evaluate the association between sacubitril/valsartan and dementia-related adverse events (AEs) in geographical subpopulations using subgroup disproportionality analysis. Cases from the FDA adverse event reporting system involving patients aged 60 or older with sacubitril/valsartan or angiotensin receptor blockers (ARBs) were analyzed. The adjusted reporting odds ratios (RORs) for dementia-related AEs were calculated for each continent. A total of 61,518 AEs associated with sacubitril/valsartan or ARBs were identified. Among these, 1441 were dementia-related AEs. In Asia, Europe, and Africa, the reporting risk of dementia-related AEs associated with sacubitril/valsartan was lower compared to ARBs (adjusted ROR, 0.57 [95% CI 0.31–1.01]; adjusted ROR, 0.89 [95% CI 0.69–1.14]; adjusted ROR, 0.40 [95% CI 0.27–0.61], respectively). In Latin America and Oceania, the reporting risk of dementia-related AEs associated with sacubitril/valsartan was similar to that associated with ARBs (adjusted ROR, 1.04 [95% CI 0.75–1.44]; adjusted ROR, 1.02 [95% CI 0.31–3.37], respectively). On the contrary, in North America, the reporting risk associated with sacubitril/valsartan was higher compared to ARBs (adjusted ROR, 1.29 [95% CI 1.10–1.53]). Although the ROR value did not meet the criteria for signal detection, the significantly greater than 1 ROR observed in North America suggests that caution may be warranted regarding potential dementia-related adverse events associated with sacubitril/valsartan.
Psychometric properties of the Depression Anxiety Stress Scales (DASS-21) in women with breast cancer
Authors: Soria-Reyes Lorena M. , Alarcón Rafael , Cerezo María V. , Blanca María J. 
Published: September 2024

AbstractBreast cancer impacts the psychological well-being of women, leaving them at risk of developing depression, anxiety, and other stress-related disorders. The Depression Anxiety Stress Scales (DASS-21) is a widely used measure, although empirical evidence regarding its psychometric properties in the breast cancer population is limited. The purpose of this study was to conduct an exhaustive analysis of the psychometric properties of the DASS-21 in a sample of Spanish women diagnosed with breast cancer. Participants were 289 breast cancer patients who completed the DASS-21 and other questionnaires measuring life satisfaction, positive and negative affect, flourishing, perceived stress, and breast cancer-specific stressors. In terms of validity evidence based on the internal structure of the DASS-21, adequate fit indices were obtained for the model based on three first-order factors (depression, anxiety, stress) and one second-order factor (general psychological distress). Reliability coefficients (McDonald’s omega) ranged from .84 to .95. Validity evidence based on relationships with other variables was also provided by moderate and strong correlations with well-being indicators and stress measures. The results support the use of the DASS-21 for measuring general psychological distress in the breast cancer context, where it may provide useful information for the design of psychological interventions with patients.
Renewable synthesis of MoO3 nanosheets via low temperature phase transition for supercapacitor application
Authors: Amba Sankar K. N. , Kesavan Lokesh , Saha Bikash , Jyolsnaraj M. K. , Mohan S. , Nandakumar P. , Mohanta Kallol , Kvarnström Carita 
Published: September 2024

Abstract2D transition metal oxides have created revolution in the field of supercapacitors due to their fabulous electrochemical performance and stability. Molybdenum trioxides (MoO3) are one of the most prominent solid-state materials employed in energy storage applications. In this present work, we report a non-laborious physical vapor deposition (PVD) and ultrasonic extraction (USE) followed by vacuum assisted solvothermal treatment (VST) route (DEST), to produce 2D MoO3 nanosheets, without any complex equipment requirements. Phase transition in MoO3 is often achieved at very high temperatures by other reported works. But our well-thought-out, robust approach led to a phase transition from one phase to another phase, for e.g., hexagonal (h-MoO3) to orthorhombic (α-MoO3) structure at very low temperature (90 °C), using a green solvent (H2O) and renewable energy. This was achieved by implementing the concept of oxygen vacancy defects and solvolysis. The synthesized 2D nanomaterials were investigated for electrochemical performance as supercapacitor electrode materials. The α-MoO3 electrode material has shown supreme capacitance (256 Fg−1) than its counterpart h-MoO3 and mixed phases (h and α) of MoO3 (< 50 Fg−1). Thus, this work opens up a new possibility to synthesize electrocapacitive 2D MoO3 nanosheets in an eco-friendly and energy efficient way; hence can contribute in renewable circular economy.
An improved Coati Optimization Algorithm with multiple strategies for engineering design optimization problems
Authors: Qi Zhang , Yingjie Dong , Shan Ye , Xu Li , Dongcheng He , Guoqi Xiang 
Published: September 2024

AbstractAiming at the problems of insufficient ability of artificial COA in the late optimization search period, loss of population diversity, easy to fall into local extreme value, resulting in slow convergence and lack of exploration ability; In this paper, an improved COA algorithm based on chaotic sequence, nonlinear inertia weight, adaptive T-distribution variation strategy and alert updating strategy is proposed to enhance the performance of COA (shorted as TNTWCOA). The algorithm introduces chaotic sequence mechanism to initialize the position. The position distribution of the initial solution is more uniform, the high quality initial solution is generated, the population richness is increased, and the problem of poor quality and uneven initial solution of the Coati Optimization Algorithm is solved. In exploration phase, the nonlinear inertial weight factor is introduced to coordinate the local optimization ability and global search ability of the algorithm. In the exploitation phase, adaptive T-distribution variation is introduced to increase the diversity of individual population under low fitness value and improve the ability of the algorithm to jump out of the local optimal value. At the same time, the alert update mechanism is proposed to improve the alert ability of COA algorithm, so that it can search within the optional range. When Coati is aware of the danger, Coati on the edge of the population will quickly move to the safe area to obtain a better position, while Coati in the middle of the population will randomly move to get closer to other Coatis. IEEE CEC2017 with 29 classic test functions were used to evaluate the convergence speed, convergence accuracy and other indicators of TNTWCOA algorithm. Meanwhile, TNTWCOA was used to verify 4 engineering design optimization problems, such as pressure vessel optimization design and welding beam design. The results of IEEE CEC2017 and engineering design Optimization problems are compared with Improved Coati Optimization Algorithm (ICOA), Coati Optimization Algorithm (COA), Golden Jackal Optimization Algorithm (GJO), Osprey Optimization Algorithm (OOA), Sand Cat Swarm Optimization Algorithm (SCSO), Subtraction-Average-Based Optimizer (SABO). The experimental results show that the improved TNTWCOA algorithm significantly improves the convergence speed and optimization accuracy, and has good robustness. Three‑bar truss design problem, The Gear Train Design Problem, Speed reducer design problem shows a strong solution advantage. The superior optimization ability and engineering practicability of TNTWCOA algorithm are verified.
Differential effects of hypoxia on motility using various in vitro models of lung adenocarcinoma
Authors: Surguta Sára Eszter , Baranyi Marcell , Svajda Laura , Cserepes Mihály , Ranđelović Ivan , Tátrai Enikő , Hegedűs Balázs , Tóvári József 
Published: September 2024

AbstractLung cancer is the leading cause of cancer-related death globally. Metastasis is the most common reason of mortality in which hypoxia is suggested to have a pivotal role. However, the effect of hypoxia on the metastatic potential and migratory activity of cancer cells is largely unexplored and warrants detailed scientific investigations. Accordingly, we analyzed changes on cell proliferation and migratory activity both in single-cell migration and invasion under normoxic and hypoxic conditions in lung adenocarcinoma cell lines. Alterations in crucial genes and proteins associated with cellular response to hypoxia, epithelial-mesenchymal transition, proliferation and apoptosis were also analyzed. Generally, we observed no change in proliferation upon hypoxic conditions and no detectable induction of apoptosis. Interestingly, we observed that single-cell motility was generally reduced while invasion under confluent conditions using scratch assay was enhanced by hypoxia in most of the cell lines. Furthermore, we detected changes in the expression of EMT markers that are consistent with enhanced motility and metastasis-promoting effect of hypoxia. In summary, our study indicated cell line-, time of exposure- and migrational type-dependent effects of hypoxia in cellular proliferation, motility and gene expression. Our results contribute to better understanding and tackling cancer metastasis.
World economies’ progress in decoupling from CO2 emissions
Authors: Freire-González Jaume , Padilla Rosa Emilio , Raymond Josep Ll. 
Published: September 2024

AbstractThe relationship between economic growth and CO2 emissions has been analyzed testing the environmental Kuznets curve hypothesis, but traditional econometric methods may be flawed. An alternative method is proposed using segmented-sample regressions and implemented in 164 countries (98.34% of world population) over different periods from 1822 to 2018. Results suggest that while the association between GDP per capita and CO2 emissions per capita is weakening over time, it remains positive globally, with only some high-income countries showing a reversed association in recent years. While 49 countries have decoupled emissions from economic growth, 115 have not. Most African, American, and Asian countries have not decoupled, whereas most European and Oceanians have. These findings highlight the urgency for effective climate policies because decoupling remains unachieved on a global scale, and we are moving away from, rather than approaching, the Paris Agreement goal of limiting temperature increase to 1.5 °C above preindustrial levels.
Computer tomography-based assessment of perivascular adipose tissue in patients with abdominal aortic aneurysms
Authors: Ginzburg Daniel , Nowak Sebastian , Attenberger Ulrike , Luetkens Julian , Sprinkart Alois Martin , Kuetting Daniel 
Published: September 2024

AbstractThis retrospective study investigates perivascular adipose tissue (PVAT) alterations in CT as a marker of inflammation in patients with abdominal aortic aneurysms (AAA). 100 abdominal CT scans of patients with abdominal aortic aneurysms and 100 age and sex matched controls without underlying aortic disease were included. Artificial Intelligence (AI) assisted segmentation of the aorta and the surrounding adipose tissue was performed. Adipose tissue density was measured in Hounsfield units (HU) close (2-5mm, HUclose) and distant (10-12mm, HUdistant) to the aortic wall. To investigate alterations in adipose tissue density close to the aorta (HUclose) as a potential marker of inflammation, we calculated the difference HUΔ = HUclose-HUdistant and the fat attenuation ratio HUratio = HUclose/HUdistant as normalized attenuation measures. These two markers were compared i) inter-individually between AAA patients and controls and ii) intra-individually between the aneurysmal and non-aneurysmal segments in AAA patients. Since most AAAs are generally observed infrarenal, the aneurysmal section of the AAA patients was compared with the infrarenal section of the aorta of the control patients. In inter-individual comparisons, higher HUΔ and a lower HUratio were observed (aneurysmal: 8.9 ± 5.1 HU vs. control: 6.9 ± 4.8 HU, p-value = 0.006; aneurysmal: 89.8 ± 5.7% vs. control: 92.1 ± 5.5% p-value = 0.004). In intra-individual comparisons, higher HUΔ and lower HUratio were observed (aneurysmal: 8.9 ± 5.1 HU vs. non-aneurysmal: 5.5 ± 4.1 HU, p-value < 0.001; aneurysmal: 89.8 ± 5.7% vs. non-aneurysmal 93.3 ± 4.9%, p-value < 0.001). The results indicate PVAT density alterations in AAA patients. This motivates further research to establish non-invasive imaging markers for vascular and perivascular inflammation in AAA.
Relationship between binocular vision and Govetto’s stage in monocular idiopathic epiretinal membrane
Authors: Tsuda Kanae , Miyata Manabu , Kawai Kentaro , Nakao Shinya , Yamamoto Akinari , Suda Kenji , Nakano Eri , Tagawa Miho , Muraoka Yuki , Sakata Ryo , Tsujikawa Akitaka 
Published: September 2024

AbstractGovetto’s staging system (stages 1–4) for epiretinal membrane (ERM) based on optical coherence tomography images is a useful predictor of monocular visual function; however, an association between Govetto’s stage and binocular vision has not been reported. This study aimed to investigate the factors associated with Govetto’s stage among the monocular and binocular parameters. This retrospective study included consecutive patients with treatment-naïve eyes with unilateral ERM without pseudo-hole. We investigated Govetto’s stage, degrees of aniseikonia and metamorphopsia, foveal avascular zone area, central retinal and choroidal thickness, vertical ocular deviation, stereopsis, and binocular single vision (BSV). We compared the parameters between the BSV-present and BSV-absent groups and investigated correlations between Govetto’s stage and the monocular and binocular parameters. Twenty-eight eyes of 28 patients were examined (age, 66.6 ± 10.2 years). In multivariate correlation analyses, Govetto’s stage correlated with BSV (P = 0.04, β = − 0.36) and central retinal thickness (P < 0.001, β = 0.74). Of the eyes, 18 were assigned to the BSV-present group and 10 to the BSV-absent group. Govetto’s stage was significantly more advanced in the BSV-absent group than in the BSV-present group (3.2 ± 0.8 vs 2.5 ± 0.7, P = 0.03). Of the 28 patients, 11 (39%) showed small-angle vertical deviations (1–12Δ). In conclusion, our findings showed that Govetto’s stage correlated with binocular vision in patients with monocular ERM. Govetto’s staging is a useful parameter for predicting not only monocular but also binocular vision.
Mechanism of activation and autophosphorylation of a histidine kinase
Authors: Kansari Mayukh , Idiris Fathia , Szurmant Hendrik , Kubař Tomáš , Schug Alexander 
Published: September 2024

AbstractHistidine kinases (HK) are one of the main prokaryotic signaling systems. Two structurally conserved catalytic domains inside the HK enable autokinase, phosphotransfer, and phosphatase activities. Here, we focus on a detailed mechanistic understanding of the functional cycle of the WalK HK by a multi-scale simulation approach, consisting of classical as well as hybrid QM/MM molecular dynamics simulation. Strikingly, a conformational transition induced solely in DHp leads to the correct activated conformation in CA crucial for autophosphorylation. This finding explains how variable sensor domains induce the transition from inactive to active state. The subsequent autophosphorylation inside DHp proceeds via a penta-coordinated transition state to a protonated phosphohistidine intermediate. This intermediate is consequently deprotonated by a suitable nearby base. The reaction energetics are controlled by the final proton acceptor and presence of a magnesium cation. The slow rates of the process result from the high energy barrier of the conformational transition between inactive and active states. The phosphorylation step exhibits a lower barrier and down-the-hill energetics. Thus, our work suggests a detailed mechanistic model for HK autophosphorylation.
Controlling enzyme hydrolysis of branched polymers synthesised using transfer-dominated branching radical telomerisation via telogen and taxogen selection
Authors: Mckeating Samuel , Penrhyn-Lowe Oliver B. , Flynn Sean , Cassin Savannah R. , Lomas Sarah , Fidge Christopher , Price Paul , Wright Stephen , Chambon Pierre , Rannard Steve P. 
Published: September 2024

AbstractWith the ever-growing reliance on polymeric materials for numerous applications, new avenues to induce, design and control degradation are clearly important. Here, we describe a previously unreported approach to controlling enzymatic hydrolysis of high molecular weight branched polymers formed from the new free-radical polymer synthesis strategy transfer-dominated branching radical telomerisation (TBRT). Modifying the chemical nature of TBRT polymers may be accomplished through telogen selection and multi-vinyl taxogen (MVT) design, and we show telogen-driven control of enzyme-catalysed hydrolysis and the impact of careful placement of hydrolytically susceptible groups within readily synthesised MVTs. Our results indicate that utilising conventional free-radical chemistries and unsaturated monomers as feedstocks for highly branched polymer architectures has considerable potential for the design of future materials that degrade into very low molecular weight byproducts at variable and controllable rates.
International cooperation was key to stabilize wheat prices after the Russian Invasion of Ukraine
Authors: Kuhla Kilian , Puma Michael J. , Otto Christian 
Published: September 2024

AbstractThe Russian invasion of Ukraine in 2022 triggered a global wheat price spike and food insecurities in import-dependent countries. We combine an analysis of the global wheat supply network with an agricultural commodity price model to investigate national impaired supplies and the global annual wheat price hike, respectively, for the trade year 2022. Using a scenario analysis, we show that international cooperation manifested in the Black Sea Grain and Solidarity Lanes initiatives and the removal of export restrictions may have mitigated the 2022 price hike by 13 percentage points. In a worst case scenario – characterized by multi-breadbasket harvest failures, escalating export restrictions, and blocked Ukrainian exports – wheat price increases by 90% compared to the 2000–2020 average. Coping strategies – such as food-secure countries dispersing stocks, reducing wheat as feed, or boosting wheat production – are effective at mitigating the price spike in simplified scenarios. Our findings underscore the imperative of coordinated policy responses to avoid global food supply disruptions.