bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is a chlorine dioxide wiping procedure suitable for the high-level disinfection of nasendoscopes?
Authors: Henoun Loukili Noureddine , Lemaitre Nadine , Guery Benoit , Gaillot Olivier , Chevalier Dominique , Mortuaire Geoffrey 
Published: January 2017

Background: Nasendoscopes are widely used in the outpatient ENT setting. Their reprocessing requires high-level disinfection (HLD). Recently, a wiping procedure using chlorine dioxide (ClO2) has been proposed as an alternative to HLD traditional procedures. Objective: To assess the effectiveness of the HLD wiping procedure versus soaking procedure on a contaminated nasendoscope. Method: A nasendoscope was contaminated with four strains of bacteria and Bacillus subtilis spores. After HLD either with the wiping procedure or with the soaking procedure (PA), the reduction of the initial contamination was determined. Findings: The wiping procedure with ClO2 displayed more than 5 log reduction for vegetative bacteria after 30 s contact time (CT) and 4 log reduction on B. subtilis spores after 2 min CT. The soaking procedure with PA displayed similar results on planktonic bacteria after 10 min CT but the log reduction of B. subtilis remained below 4. Conclusion: The ClO2 wiping procedure showed bactericidal and sporicidal efficacy on a contaminated nasendoscope in a shorter time compared to the PA soaking procedure. Thus, ClO2 wiping procedure might be considered as an alternative to the traditional HLD procedure for nasendoscopes.
Quantitative stability of hematopoietic stem and progenitor cell clonal output in rhesus macaques receiving transplants
Authors: Koelle Samson J. , Espinoza Diego A. , Wu Chuanfeng , Xu Jason , Lu Rong , Li Brian , Donahue Robert E. , Dunbar Cynthia E. 
Published: January 2017

Key Points
Output from individual rhesus macaque hematopoietic stem and progenitor cells is stable for years, with little evidence of clonal succession. Individual clones may display stable myeloid or lymphoid bias for many years.
International, evidence-based consensus diagnostic criteria for HHV-8–negative/idiopathic multicentric Castleman disease
Authors: Fajgenbaum David C. , Uldrick Thomas S. , Bagg Adam , Frank Dale , Wu David , Srkalovic Gordan , Simpson David , Liu Amy Y. , Menke David , Chandrakasan Shanmuganathan , Lechowicz Mary Jo , Wong Raymond S. M. , Pierson Sheila , Paessler Michele , Rossi Jean-François , Ide Makoto , Ruth Jason , Croglio Michael , Suarez Alexander , Krymskaya Vera , Chadburn Amy , Colleoni Gisele , Nasta Sunita , Jayanthan Raj , Nabel Christopher S. , Casper Corey , Dispenzieri Angela , Fosså Alexander , Kelleher Dermot , Kurzrock Razelle , Voorhees Peter , Dogan Ahmet , Yoshizaki Kazuyuki , van Rhee Frits , Oksenhendler Eric , Jaffe Elaine S. , Elenitoba-Johnson Kojo S. J. , Lim Megan S. 
Published: January 2017

Key Points
An international panel established the first ever diagnostic criteria for iMCD based on review of 244 clinical cases and 88 tissue samples. The criteria require multicentric lymphadenopathy with defined histopathology, ≥2 clinical/laboratory changes, and exclusion of iMCD mimics.
Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method
Authors: Yang Shuang , Höti Naseruddin , Yang Weiming , Liu Yang , Chen Lijun , Li Shuwei , Zhang Hui 
Published: January 2017

Abstract
Background
Glycans play critical roles in a number of biological activities. Two common types of glycans, N-linked and O-linked, have been extensively analyzed in the last decades. N-glycans are typically released from glycoproteins by enzymes, while O-glycans are released from glycoproteins by chemical methods. It is important to identify and quantify both N- and O-linked glycans of glycoproteins to determine the changes of glycans.

Methods
The effort has been dedicated to study glycans from ovarian cancer cells treated with O-linked glycosylation inhibitor qualitatively and quantitatively. We used a solid-phase chemoenzymatic approach to systematically identify and quantify N-glycans and O-glycans in the ovarian cancer cells. It consists of three steps: (1) immobilization of proteins from cells and derivatization of glycans to protect sialic acids; (2) release of N-glycans by PNGase F and quantification of N-glycans by isobaric tags; (3) release and quantification of O-glycans by β-elimination in the presence of 1-phenyl-3-methyl-5-pyrazolone (PMP).

Results
We used ovarian cancer cell lines to study effect of O-linked glycosylation inhibitor on protein glycosylation. Results suggested that the inhibition of O-linked glycosylation reduced the levels of O-glycans. Interestingly, it appeared to increase N-glycan level in a lower dose of the O-linked glycosylation inhibitor. The sequential release and analyses of N-linked and O-linked glycans using chemoenzymatic approach are a platform for studying N-glycans and O-glycans in complex biological samples.

Conclusion
The solid-phase chemoenzymatic method was used to analyze both N-linked and O-linked glycans sequentially released from the ovarian cancer cells. The biological studies on O-linked glycosylation inhibition indicate the effects of O-glycosylation inhibition to glycan changes in both O-linked and N-linked glycan expression.
Diabetes does not impact the diagnostic performance of contrast-based fractional flow reserve: insights from the CONTRAST study
Authors: Gargiulo Giuseppe , Stabile Eugenio , Ferrone Marco , Barbato Emanuele , Zimmermann Frederik M. , Adjedj Julien , Hennigan Barry , Matsumura Mitsuaki , Johnson Nils P. , Fearon William F. , Jeremias Allen , Trimarco Bruno , Esposito Giovanni ,   On behalf of the CONTRST Study Investigators
Published: January 2017

Abstract
Background
Adenosine-free coronary pressure wire metrics have been proposed to test the functional significance of coronary artery lesions, but it is unexplored whether their diagnostic performance might be altered in patients with diabetes.

Methods
We performed a post-hoc analysis of the CONTRAST study, which prospectively enrolled an international cohort of patients undergoing routine fractional flow reserve (FFR) assessment for standard indications. Paired, repeated measurements of all physiology metrics (Pd/Pa, iFR, contrast-based FFR, and FFR) were made. A central core laboratory analyzed blinded pressure tracings in a standardized fashion.

Results
Of 763 subjects enrolled at 12 international centers, 219 (29%) had diabetes. The two groups were well-balanced for age, clinical presentation (stable or unstable), coronary vessel studied, volume and type of intracoronary contrast, and volume of intracoronary adenosine. A binary threshold of cFFR ≤ 0.83 produced an accuracy superior to both Pd/Pa and iFR when compared with FFR ≤ 0.80 in the absence of significant interaction with diabetes status; indeed, accuracy in subgroups of patients with or without diabetes was similar for cFFR (86.7 vs 85.4% respectively; p = 0.76), iFR (84.2 vs 80.0%, p = 0.29) and Pd/Pa (81.3 vs 78.9%, p = 0.55). There was no significant heterogeneity between patients with or without diabetes in terms of sensitivity and specificity of all metrics. The area under the receiver operating characteristic (ROC) curve was largest for cFFR compared with Pd/Pa and iFR which were equivalent (cFFR 0.961 and 0.928; Pd/Pa 0.916 and 0.870; iFR 0.911 and 0.861 in diabetic and non-diabetic patients respectively).

Conclusions
cFFR provides superior diagnostic performance compared with Pd/Pa or iFR for predicting FFR irrespective of diabetes (clinicaltrials.gov identifier NCT02184117).
Comparison of two adult mosquito sampling methods with human landing catches in south-central Ethiopia
Authors: Kenea Oljira , Balkew Meshesha , Tekie Habte , Gebre-Michael Teshome , Deressa Wakgari , Loha Eskindir , Lindtjørn Bernt , Overgaard Hans J. 
Published: January 2017

Abstract
Background
The human landing catch (HLC) is the standard reference method for measuring human exposure to mosquito bites. However, HLC is labour-intensive, exposes collectors to infectious mosquito bites and is subjected to collector bias. These necessitate local calibration and application of alternative methods. This study was undertaken to determine the relative sampling efficiency (RSE) of light traps with or without yeast-produced carbon dioxide bait vs. HLC in south-central Ethiopia.

Methods
The experiment was conducted for 39 nights in a 3 × 3 Latin square randomized design with Anopheles arabiensis as the target species in the period between July and November 2014 in Edo Kontola village, south-central Ethiopia. Center for Disease Control and Prevention light trap catches (LTC) and yeast-generated carbon dioxide-baited light trap catches (CB-LTC) were each evaluated against HLC. The total nightly mosquito catches for each Anopheles species in either method was compared with HLC by Pearson correlation and simple linear regression analysis on log-transformed [log10(x + 1)] values. To test if the RSE of each alternative method was affected by mosquito density, the ratio of the number of mosquitoes in each method to the number of mosquitoes in HLC was plotted against the average mosquito abundance.

Results
Overall, 7606 Anopheles females were collected by the three sampling methods. Among these 5228 (68.7%) were Anopheles ziemanni, 1153 (15.2%) An. arabiensis, 883 (11.6%) Anopheles funestus s.l., and 342 (4.5%) Anopheles pharoensis. HLC yielded 3392 (44.6%), CB-LTC 2150 (28.3%), and LTC 2064 (27.1%) Anopheles females. The RSEs of LTC and HLC for An. arabiensis were significantly correlated (p < 0.001) and density independent (p = 0.65). However, for outdoor collection of the same species, RSEs of LTC and CB-LTC were density dependent (p < 0.001). It was estimated that on average, indoor LTC and CB-LTC each caught 0.35 and 0.44 times that of indoor HLC for An. arabiensis respectively.

Conclusions
Results showed that HLC was the most efficient method for sampling An. arabiensis. LTC can be used for large-scale indoor An. arabiensis surveillance and monitoring when it is difficult to use HLC. CB-LTC does not substantially improve sampling of this major vector compared to LTC in this setting.
Trial registration PACTR201411000882128 (retrospectively registered 8 September, 2014)
Effects of the intradiscal implantation of stromal vascular fraction plus platelet rich plasma in patients with degenerative disc disease
Authors: Comella Kristin , Silbert Robert , Parlo Michelle 
Published: January 2017

Abstract
Background
Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF and PRP intra-discally into patients with degenerative disc disease.

Methods
A total of 15 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue. The fat was separated to isolate the SVF and the cells were delivered into the disc nucleus of patients with degenerative disc disease. The subjects were then monitored for adverse events, range of motion, visual analog scale (VAS), present pain intensity (PPI), Oswestry Disability Index (ODI), Beck Depression Inventory (BDI), Dallas Pain Questionnaire and Short Form (SF)-12 scores over a period of 6 months. Safety events were followed for 12 months.

Results
No severe adverse events (SAEs) were reported during a 12 month follow up period with no incidences of infection. Patients demonstrated statistically significant improvements in several parameters including flexion, pain ratings, VAS, PPI, and short form questionnaires. In addition, both ODI and BDI data was trending positive and a majority of patients reported improvements in their Dallas Pain Questionnaire scores.

Conclusions
Overall, patients were pleased with the treatment results. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications linked to the therapy.
Trial registration NCT02097862. Name of registry: www.clinicaltrials.gov. https://clinicaltrials.gov/ct2/show/NCT02097862?term=bioheart&rank=6. Date of registration: March 25, 2014; Date of enrollment: March 2014
Using Intervention Mapping to develop the Parents as Agents of Change (PAC©) intervention for managing pediatric obesity
Authors: Ball Geoff D. C. , Mushquash Aislin R. , Keaschuk Rachel A. , Ambler Kathryn A. , Newton Amanda S. 
Published: January 2017

Abstract
Background
Pediatric obesity has become increasingly prevalent over recent decades. In view of the psychosocial and physical health risks, and the high likelihood that children with obesity will grow to become adults with obesity, there is a clear need to develop evidence-based interventions that can be delivered in the health care system to optimize the health and well-being of children with obesity and their families. The aim of this paper is to describe the development, implementation, and planned evaluation of a parent-based weight management intervention designed for parents of 8–12 year olds with obesity.

Methods/results
The principles of Intervention Mapping (IM) were used to develop an intervention called Parents as Agents of Change (PAC©). From 2006 to 2009, an environmental scan plus qualitative (individual interviews with parents and children), quantitative (medical record reviews), and literature review data were collected to gain broad insight into family factors related to pediatric obesity and its management. Theoretical frameworks and empirical evidence guided curriculum development, which was founded primarily on the tenets of family systems theory and cognitive behavioral theory. PAC was developed as a manualized, 16-session, group-based, health care professional-led intervention for parents to address individual, family, and environmental factors related to the management of pediatric obesity. The intervention was refined based on feedback from local and international experts, and has been implemented successfully in a multi-disciplinary weight management centre in a children’s hospital.

Conclusion
IM provided a practical framework to guide the systematic development of a pediatric weight management intervention for parents of children with obesity. This logical, step-by-step process blends theory and practice and is broadly applicable in the context of obesity management intervention development and evaluation. Following intervention development, the PAC intervention was evaluated within a randomized clinical trial.
Trial registration NCT01267097; clinicaltrials.gov
Role of the phagosomal redox-sensitive TRP channel TRPM2 in regulating bactericidal activity of macrophages
Authors: Di Anke , Kiya Tomohiro , Gong Haixia , Gao Xiaopei , Malik Asrar B. 
Published: January 2017

ABSTRACT
Acidification of macrophage phagosomes serves an important bactericidal function. We show here that the redox-sensitive transient receptor potential (TRP) cation channel TRPM2 is expressed in the phagosomal membrane and regulates macrophage bactericidal activity through the activation of phagosomal acidification. Measurement of the TRPM2 current in phagosomes identified TRPM2 as a functional redox-sensitive cation channel localized in the phagosomal membrane. Simultaneous measurements of phagosomal Ca2+ changes and phagosome acidification in macrophages undergoing phagocytosis demonstrated that TRPM2 was required to mediate the efflux of cations and for phagosomal acidification during the process of phagosome maturation. Acidification in phagosomes was significantly reduced in macrophages isolated from Trpm2−/− mice as compared to wild type, and acidification was coupled to reduced bacterial clearance in Trpm2−/− mice. Trpm2+/+ macrophages treated with the vacuolar H+-ATPase inhibitor bafilomycin showed reduced bacterial clearance, similar to that in Trpm2−/− macrophages. Direct activation of TRPM2 using adenosine diphosphate ribose (ADPR) induced both phagosomal acidification and bacterial killing. These data collectively demonstrate that TRPM2 regulates phagosomal acidification, and is essential for the bacterial killing function of macrophages.
Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development
Authors: Behm Mikaela , Wahlstedt Helene , Widmark Albin , Eriksson Maria , Öhman Marie 
Published: January 2017

ABSTRACT
Adenosine to inosine (A-to-I) RNA editing is important for a functional brain, and most known sites that are subject to selective RNA editing have been found to result in diversified protein isoforms that are involved in neurotransmission. In the absence of the active editing enzymes ADAR1 or ADAR2 (also known as ADAR and ADARB1, respectively), mice fail to survive until adulthood. Nuclear A-to-I editing of neuronal transcripts is regulated during brain development, with low levels of editing in the embryo and a dramatic increase after birth. Yet, little is known about the mechanisms that regulate editing during development. Here, we demonstrate lower levels of ADAR2 in the nucleus of immature neurons than in mature neurons. We show that importin-α4 (encoded by Kpna3), which increases during neuronal maturation, interacts with ADAR2 and contributes to the editing efficiency by bringing it into the nucleus. Moreover, we detect an increased number of interactions between ADAR2 and the nuclear isomerase Pin1 as neurons mature, which contribute to ADAR2 protein stability. Together, these findings explain how the nuclear editing of substrates that are important for neuronal function can increase as the brain develops.
An ELISA DYRK1A non-radioactive assay suitable for the characterization of inhibitors
Authors: Liu Yong , Adayev Tatyana , Hwang Yu-Wen 
Published: January 2017

The DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1A) gene encodes a proline-directed Ser/Thr kinase. Elevated expression and/or altered distribution of the kinase have been implicated in the neurological impairments associated with Down syndrome (DS) and Alzheimer’s disease (AD). Consequently, DYRK1A inhibition has been of significant interest as a potential strategy for therapeutic intervention of DS and AD. Many classes of novel inhibitors have been described in the past decade. Although non-radioactive methods for analyzing DYRK1A inhibition have been developed, methods employing radioactive tracers are still commonly used for quantitative characterization of DYRK1A inhibitors. Here, we present a non-radioactive ELISA assay based on the detection of DYRK1A-phosphorylated dynamin 1a fragment using a phosphorylation site-specific antibody. The assay was verified by the use of two well-characterized DYRK1A inhibitors, epigallocatechin gallate (EGCG) and harmine. The IC50s for EGCG and harmine determined by the ELISA method were found to be comparable to those previously measured by radioactive tracing methods. Furthermore, we determined the mode of inhibition for EGCG and harmine by a modification of the ELISA assay. This assay confirms the mode of inhibition of EGCG (non-ATP-competitive) and harmine (ATP-competitive), as previously determined. We conclude that the ELISA platform demonstrated here is a viable alternative to the traditional radioactive tracer assays for analyzing DYRK1A inhibitors.
Digital Methodology to implement the ECOUTER engagement process
Authors: Wilson Rebecca C. , Butters Oliver W. , Clark Tom , Minion Joel , Turner Andrew , Murtagh Madeleine J. 
Published: January 2017

ECOUTER (Employing COnceptual schema for policy and Translation E in Research – French for ‘to listen’ – is a new stakeholder engagement method incorporating existing evidence to help participants draw upon their own knowledge of cognate issues and interact on a topic of shared concern. The results of an ECOUTER can form the basis of recommendations for research, governance, practice and/or policy. This paper describes the development of a digital methodology for the ECOUTER engagement process based on currently available mind mapping freeware software. The implementation of an ECOUTER process tailored to applications within health studies are outlined for both online and face-to-face scenarios. Limitations of the present digital methodology are discussed, highlighting the requirement of a purpose built software for ECOUTER research purposes.
A Data Assimilation Algorithm for the Subcritical Surface Quasi-Geostrophic Equation
Authors: Jolly Michael S. , Martinez Vincent R. , Titi Edriss S. 
Published: January 2017

Abstract
In this article, we prove that data assimilation by feedback nudging can be achieved for the three-dimensional quasi-geostrophic equation in a simplified scenario using only large spatial scale observables on the dynamical boundary. On this boundary, a scalar unknown (buoyancy or surface temperature of the fluid) satisfies the surface quasi-geostrophic equation. The feedback nudging is done on this two-dimensional model, yet ultimately synchronizes the streamfunction of the three-dimensional flow. The main analytical difficulties are due to the presence of a nonlocal dissipative operator in the surface quasi-geostrophic equation. This is overcome by exploiting a suitable partition of unity, the modulus of continuity characterization of Sobolev space norms, and the Littlewood–Paley decomposition to ultimately establish various boundedness and approximation-of-identity properties for the observation operators.
Structural symmetry within nonlocal integral elasticity: theoretical issues and computational strategies
Authors: Pisano Aurora Angela , Fuschi Paolo 
Published: January 2017

Abstract The structural symmetry and the appropriate definition of a reduced (symmetric) mechanical/ numerical model is discussed within a nonlocal elasticity context. In particular, reference is made to an integral model of Eringen-type. The paper highlights how the classical, i.e. local, concepts of structural symmetry have to be rephrased through the definition of an enlarged symmetric model of the analyzed structure. This enlarged model, endowed with apposite nonlocal boundary conditions enforced in an iterative fashion, is proved to be able to recover the nonlocal effects that the neglected portion of the structure exerts on the portion chosen for the analysis. It is shown how the mirrored symmetric solution exactly matches the complete one. Theoretical issues and computational strategies referred to a nonlocal version of the finite element method are discussed with reference to the analysis of a case-study.
Displacement and stress analysis of laminated composite plates using an eight-node quasi-conforming solid-shell element
Authors: Wang Yu , Shi Guangyu , Wang Xiaodan 
Published: January 2017

Abstract This paper presents the efficient modeling and analysis of laminated composite plates using an eightnode quasi-conforming solid-shell element, named as QCSS8. The present element QCSS8 is not only lockingfree, but highly computational efficiency as it possesses the explicit element stiffness matrix. All the six components of stresses can be evaluated directly by QCSS8 in terms of the 3-D constitutive equations and the appropriately assumed element strain field. Several typical numerical examples of laminated plates are solved to validate QCSS8, and the resulting values are compared with analytical solutions and the numerical results of solid/solidshell elements of commercial codes computed by the present authors in which fine meshes were used. The numerical results show that QCSS8 can give accurate displacements and stresses of laminated composite plates even with coarse meshes. Furthermore, QCSS8 yields also accurate transverse normal strain which is very important for the evaluation of interlaminar stresses in laminated plates. Since each lamina of laminated composite plates can be modeled naturally by one or a few layers of solidshell elements and a large aspect ratio of element edge to thickness is allowed in solid-shell elements, the present solid-shell element QCSS8 is extremely appropriate for the modeling of laminated composite plates.