bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphorylation-driven epichaperome assembly is a regulator of cellular adaptability and proliferation
Authors: Roychowdhury Tanaya , McNutt Seth W. , Pasala Chiranjeevi , Nguyen Hieu T. , Thornton Daniel T. , Sharma Sahil , Botticelli Luke , Digwal Chander S. , Joshi Suhasini , Yang Nan , Panchal Palak , Chakrabarty Souparna , Bay Sadik , Markov Vladimir , Kwong Charlene , Lisanti Jeanine , Chung Sun Young , Ginsberg Stephen D. , Yan Pengrong , De Stanchina Elisa , Corben Adriana , Modi Shanu , Alpaugh Mary L. , Colombo Giorgio , Erdjument-Bromage Hediye , Neubert Thomas A. , Chalkley Robert J. , Baker Peter R. , Burlingame Alma L. , Rodina Anna , Chiosis Gabriela , Chu Feixia 
Published: October 2024

AbstractThe intricate network of protein-chaperone interactions is crucial for maintaining cellular function. Recent discoveries have unveiled the existence of specialized chaperone assemblies, known as epichaperomes, which serve as scaffolding platforms that orchestrate the reconfiguration of protein-protein interaction networks, thereby enhancing cellular adaptability and proliferation. This study explores the structural and regulatory aspects of epichaperomes, with a particular focus on the role of post-translational modifications (PTMs) in their formation and function. A key finding is the identification of specific PTMs on HSP90, particularly at residues Ser226 and Ser255 within an intrinsically disordered region, as critical determinants of epichaperome assembly. Our data demonstrate that phosphorylation of these serine residues enhances HSP90’s interactions with other chaperones and co-chaperones, creating a microenvironment conducive to epichaperome formation. Moreover, we establish a direct link between epichaperome function and cellular physiology, particularly in contexts where robust proliferation and adaptive behavior are essential, such as in cancer and pluripotent stem cell maintenance. These findings not only provide mechanistic insights but also hold promise for the development of novel therapeutic strategies targeting chaperone assemblies in diseases characterized by epichaperome dysregulation, thereby bridging the gap between fundamental research and precision medicine.
Female calls promote song learning in male juvenile zebra finches
Authors: Bistere Linda , Gomez-Guzman Carlos M. , Xiong Yirong , Vallentin Daniela 
Published: October 2024

AbstractSocial interactions promote vocal learning, but the impact of social feedback on this process and its neural circuitry is not well understood. We studied song imitation in juvenile male zebra finches raised either in the presence or absence of adult females. Juveniles learned songs more accurately with a female present, suggesting her presence improves imitation. When female calls correlated with practice, tutees’ songs better resembled the tutor’s, hinting toward the possibility that females provide practice-specific vocalizations. Intracellular recordings of HVC projection neurons revealed that a subset of these neurons in both juveniles and adults is sensitive to female calls during listening, suggesting a consistent neural mechanism for processing important vocalizations, regardless of age. However, call-related neural responses during singing were observed only in juveniles. These findings highlight how vocalizations, beyond those of the tutor, influence the neural circuits for vocal learning and production.
Prevalent and persistent new-onset autoantibodies in mild to severe COVID-19
Authors: Jernbom August F. , Skoglund Lovisa , Pin Elisa , Sjöberg Ronald , Tegel Hanna , Hober Sophia , Rostami Elham , Rasmusson Annica , Cunningham Janet L. , Havervall Sebastian , Thålin Charlotte , Månberg Anna , Nilsson Peter 
Published: October 2024

AbstractAutoantibodies have been shown to be implied in COVID-19 but the emerging autoantibody repertoire remains largely unexplored. We investigated the new-onset autoantibody repertoire in 525 healthcare workers and hospitalized COVID-19 patients at five time points over a 16-month period in 2020 and 2021 using proteome-wide and targeted protein and peptide arrays. Our results show that prevalent new-onset autoantibodies against a wide range of antigens emerged following SARS-CoV-2 infection in relation to pre-infectious baseline samples and remained elevated for at least 12 months. We found an increased prevalence of new-onset autoantibodies after severe COVID-19 and demonstrated associations between distinct new-onset autoantibodies and neuropsychiatric symptoms post-COVID-19. Using epitope mapping, we determined the main epitopes of selected new-onset autoantibodies, validated them in independent cohorts of neuro-COVID and pre-pandemic healthy controls, and identified sequence similarities suggestive of molecular mimicry between main epitopes and the conserved fusion peptide of the SARS-CoV-2 Spike glycoprotein. Our work describes the complexity and dynamics of the autoantibody repertoire emerging with COVID-19 and supports the need for continued analysis of the new-onset autoantibody repertoire to elucidate the mechanisms of the post-COVID-19 condition.
CAPTURE-24: A large dataset of wrist-worn activity tracker data collected in the wild for human activity recognition
Authors: Chan Shing , Hang Yuan , Tong Catherine , Acquah Aidan , Schonfeldt Abram , Gershuny Jonathan , Doherty Aiden 
Published: October 2024

AbstractExisting activity tracker datasets for human activity recognition are typically obtained by having participants perform predefined activities in an enclosed environment under supervision. This results in small datasets with a limited number of activities and heterogeneity, lacking the mixed and nuanced movements normally found in free-living scenarios. As such, models trained on laboratory-style datasets may not generalise out of sample. To address this problem, we introduce a new dataset involving wrist-worn accelerometers, wearable cameras, and sleep diaries, enabling data collection for over 24 hours in a free-living setting. The result is CAPTURE-24, a large activity tracker dataset collected in the wild from 151 participants, amounting to 3883 hours of accelerometer data, of which 2562 hours are annotated. CAPTURE-24 is two to three orders of magnitude larger than existing publicly available datasets, which is critical to developing accurate human activity recognition models.
The impact of explaining vegetarian meal requests on the affective responses and perceptions of meat eaters
Authors: Laffan Kate , Howard Emma 
Published: October 2024

AbstractSocial situations can present challenges for those looking to reduce or avoid eating meat. In an experimental vignette study with a large representative sample of 1,117 UK-based meat eaters, we explore affective responses to requests for vegetarian meals and perceptions of those making the request across four social situations: a BBQ, a pub, a restaurant dinner, and a dinner party. We examine moderators of these perceptions including whether the requester presents themselves as vegetarian or a meat restrictor and whether they cite health, environmental or no motives for their request. Overall, we find that these moderators have a greater impact on meat eaters’ perceptions of the requester than on their affective responses to the request. We also find that these outcomes are more impacted by the mention of motives than they are diet type. Standout findings include evidence that people citing environmental motives for their vegetarian meal request are perceived more negatively, while those with health motives are perceived more positively. We also identify substantial heterogeneity across the respondents’ levels of meat attachment: those who are more attached to meat have more negative perceptions of vegetarian meal requesters and experience higher negative affect and lower positive affect in response to the request.
Vietnamese people’s behavior and future intention to purchase medicines and functional foods on the internet: a cross-sectional study
Authors: Doan Dung Anh , Vu Nhung Hong , Nguyen Phuong Lan , Dinh Dai Xuan 
Published: October 2024

AbstractThis study investigated Vietnamese people’s behavior and future intention to purchase medicines and functional foods online and their associated factors. From March to May 2023, a Google Form questionnaire was distributed via social networks and online platforms. Convenience and snowballing sampling methods were employed to recruit 1,305 Vietnamese people. In the past year, 50.3% of participants purchased at least one kind of medicine and/or functional foods online (medicines: 27.6%, functional foods: 45.1%). Among 656 buyers, nearly a third bought these products more than three times and only 5.9% felt dissatisfied with their previous experiences. This purchasing behavior was more prevalent among females, those married, having higher education levels, usually shopping online, having longer time of Internet use per day, and seeking health information on the Internet (p < 0.001). In addition, 77.5% of participants intended to purchase these products online in the future and 61.2% would introduce this kind of online purchase to other people. The purchase intention was significantly associated with the participants’ previous experiences, area, contracting chronic diseases, and using the Internet for self-medication, while factors associated with the introducing intention included their education level, occupation, and previous experiences in online purchases (p < 0.001).
If the first child is breech, overall outcomes for families with two children are similar regardless of the mode of the first birth
Authors: Savchenko Julia , Pegelow Halvorsen Cecilia , Lindqvist Pelle G , Brismar Wendel Sophia 
Published: October 2024

AbstractCesarean section for breech presentation is often recommended. However, cesarean section affects future reproduction. The aim of this study was to assess the effect of mode of the first birth in breech on outcomes of the second birth and the two births together. This is a register-based nationwide cohort study including 23 062 women with a first singleton birth in breech ≥ 34 gestational weeks and a subsequent singleton birth in Sweden 2000–2019. Exposure was mode of first delivery. Main maternal outcome was a composite of fourth-degree perineal injury, postpartum hemorrhage requiring blood transfusion, hysterectomy, or death. Main infant outcome was a composite of stillbirth, extremely preterm birth (< 28 weeks), moderate to severe hypoxic ischemic encephalopathy, therapeutic hypothermia, or death. Outcomes were analyzed using multivariable logistic regression. In the first birth, the infant composite outcome affected < 1% in both groups but the risk was higher in the vaginal breech group (13/1525), compared with the breech CS group (27/21 537), aOR 7.06, 95% CI 2.91–17.1. In the second birth, the infant composite outcome affected < 1% in both groups but the risk was lower for the first vaginal breech group (3/1525) compared with the first breech CS group (152/21 537), aOR 0.26, 95% CI 0.08–0.84. There was no significant difference between the groups in risk of composite infant outcome in the two births assessed together, or in risk of composite maternal outcome. In total, the chance of a two-children family without maternal or infant severe adverse composite outcome was high and similar regardless mode of the breech first birth.
snPATHO-seq, a versatile FFPE single-nucleus RNA sequencing method to unlock pathology archives
Authors: Wang Taopeng , Roach Michael J. , Harvey Kate , Morlanes Javier Escudero , Kiedik Beata , Al-Eryani Ghamdan , Greenwald Alissa , Kalavros Nikolaos , Dezem Felipe Segato , Ma Yuling , Pita-Juarez Yered H. , Wise Kellie , Degletagne Cyril , Elz Anna , Hadadianpour Azi , Johanneson Jack , Pakiam Fiona , Ryu Heeju , Newell Evan W. , Tonon Laurie , Kohlway Andrew , Drennon Tingsheng , Abousoud Jawad , Stott Ryan , Lund Paul , Durruthy Jens , Vallejo Andres F. , Li Wenyan , Salomon Robert , Kaczorowski Dominik , Warren Joanna , Butler Lisa M. , O’Toole Sandra , Plummer Jasmine , Vlachos Ioannis S. , Lundeberg Joakim , Swarbrick Alexander , Martelotto Luciano G. 
Published: October 2024

AbstractFormalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low RNA quality. In this study, leveraging a recent advance in single-cell genomic technology, we introduce snPATHO-seq, a versatile method to derive high-quality single-nucleus transcriptomic data from FFPE samples. We benchmarked the performance of the snPATHO-seq workflow against existing 10x 3’ and Flex assays designed for frozen or fresh samples and highlighted the consistency in snRNA-seq data produced by all workflows. The snPATHO-seq workflow also demonstrated high robustness when tested across a wide range of healthy and diseased FFPE tissue samples. When combined with FFPE spatial transcriptomic technologies such as FFPE Visium, the snPATHO-seq provides a multi-modal sampling approach for FFPE samples, allowing more comprehensive transcriptomic characterization.
Seasonal biases in fluorescence-estimated chlorophyll-a derived from biogeochemical profiling floats
Authors: Long Jacqueline S. , Takeshita Yuichiro , Plant Joshua N. , Buzby Nina , Fassbender Andrea J. , Johnson Ken S. 
Published: October 2024

AbstractMarine phytoplankton biomass and chlorophyll-a concentration are often estimated from pigment fluorescence measurements, which have become routine despite known variability in the fluorescent response for a given amount of chlorophyll-a. Here, we present a near-global, monthly climatology of chlorophyll-a fluorescence measurements from profiling floats combined with ocean color satellite estimates of chlorophyll-a concentration to illuminate seasonal biases in the fluorescent response and expand upon previously observed regional patterns in this bias. Global biases span over an order of magnitude, and can vary seasonally by a factor of 10. An independent estimate of chlorophyll-a from light attenuation shows similar global patterns in the chlorophyll-fluorescence bias when compared to biases derived from satellite estimates. Without accounting for these biases, studies or models using fluorescence-estimated chlorophyll-a will inherit the seasonal and regional biases described here.
The e-MANTIS emulator: Fast and accurate predictions of the halo mass function in f(R)CDM and wCDM cosmologies
Authors: Sáez-Casares I. , Rasera Y. , Richardson T. R. G. , Corasaniti P.-S. 
Published: October 2024

Aims. In this work, we present a novel emulator of the halo mass function (HMF), which we implemented in the framework of the e-MANTIS emulator of f(R) gravity models. We also extended e-MANTIS to cover a larger cosmological parameter space and to include models of dark energy with a constant equation of state wCDM.
Methods. We used a Latin hypercube sampling of the wCDM and f(R)CDM cosmological parameter spaces, over a wide range, and carried out a large suite of more than 10 000 N-body simulations with a different volume, mass resolution, and random phase for the initial conditions. For each simulation in the suite, we generated halo catalogues using the friends-of-friends (FoF) halo finder, as well as the spherical overdensity (SO) algorithm for different overdensity thresholds (200, 500, and 1000 times the critical density). We decomposed the corresponding HMFs on a B-spline basis, while adopting a minimal set of assumptions on their shape. We used this decomposition to train an emulator based on Gaussian processes.
Results. The resulting emulator is able to predict the HMF for redshifts ≤1.5 and for halo masses Mh ≥ 1013 h−1 M⊙. The typical HMF errors for SO haloes with ∆ = 200c at ɀ = 0 in wCDM (respectively f(R)CDM) are of the order of ϵ0 ≃ 1.5% (ϵ0 ≃ 4%) up to a transition mass Mt ≃ 2 ⋅ 1014 h−1 M⊙ (Mt ≃ 6 ⋅ 1013 h−1 M⊙). For larger masses, the errors are dominated by the shot noise and scale as ϵ0 ⋅ (Mh/Mt)α with α ≃ 0.9 (α ≃ 0.4) up to Mh ~ 1015 h−1 M⊙. Independently of this general trend, the emulator is able to provide an estimation of its own error as a function of the cosmological parameters, halo mass, and redshift. We have performed an extensive comparison against analytical parametrizations and shown that e-MANTIS is able to better capture the cosmological dependence of the HMF, while being complementary to other existing emulators.
Conclusions. The e-MANTIS emulator, which is publicly available, can be used to obtain fast and accurate predictions of the HMF in the f(R)CDM and wCDM non-standard cosmological models. As such, it represents a useful theoretical tool to constrain the nature of dark energy using data from galaxy cluster surveys.
Magnetising galaxies with cold inflows
Authors: Ledos Nicolas , Ntormousi Evangelia , Takasao Shinsuke , Nagamine Kentaro 
Published: October 2024

Context. High-redshift (z ∼ 2 − 3) galaxies accrete circumgalactic gas through cold streams. Recent high-resolution magnetohydrodynamic (MHD) simulations of these streams show a significant amplification of the intergalactic magnetic field in the shear layer around them.
Aims. For this work we estimated the magnetisation of high-redshift galaxies that would result purely due to the accretion of already magnetised gas from cold streams.
Methods. We used the mass inflow rates and saturated magnetic field values from cold stream simulations as input to a simple analytic model that calculates the galactic magnetic field purely from mass accretion.
Results. Our model predicts average magnetic field strengths that exceed μG values at z ∼ 2 − 3 for inflow rates above 0.1 M⊙ yr−1. For high inflow rates, our model results are consistent with the recent detection of a strong magnetic field in z ≳ 2.6 galaxies.
Conclusions. Within the assumptions of our simple model, magnetised cold streams emerge as a viable mechanism for seeding a dynamically important galactic magnetic field.
SDSS J102915.14+172927.9: Revisiting the chemical pattern
Authors: Caffau E. , Bonifacio P. , Monaco L. , Steffen M. , Sbordone L. , Spite M. , François P. , Gallagher A. J. , Ludwig H.-G. , Molaro P. 
Published: October 2024

Context. The small- to intermediate-mass (M < 0.8 M⊙), most metal-poor stars that formed in the infancy of the Universe are still shining today in the sky. They are very rare, but their discovery and investigation brings new knowledge on the formation of the first stellar generations.
Aims. SDSS J102915.14+172927.9 is one of the most metal-poor star known to date. Since no carbon can be detected in its spectrum, a careful upper limit is important, both to classify this star and to distinguish it from the carbon-enhanced stars that represent the majority at these metallicities.
Methods. We undertook a new observational campaign to acquire high-resolution UVES spectra. The new spectra were combined with archival spectra in order to increase the signal-to-noise ratio. From the combined spectrum, we derived abundances for seven elements (Mg, Si, Ca, Ti, Fe, Ni, and a tentative Li) and five significant upper limits (C, Na, Al, Sr, and Ba).
Results. The star has a carbon abundance A(C) < 4.68 and therefore is not enhanced in carbon, at variance with the majority of the stars at this Fe regime, which typically show A(C) > 6.0. A feature compatible with the Li doublet at 670.7 nm is tentatively detected.
Conclusions. The upper limit on carbon implies Z < 1.915 × 10−6, more than 20 times lower than the most iron-poor star known. Therefore, the gas cloud out of which the star was formed did not cool via atomic lines but probably through dust. Fragmentation of the primordial cloud is another possibility for the formation of a star with a metallicity this low.
Semiconstrained Distal Radioulnar Joint Arthroplasty: Functional Outcomes and Complications
Authors: Pina Matthew J. , Yang Harmony Tan Chern , Pintado Gonzalo , Ozyurekoglu Tuna 
Published: October 2024

Abstract
Background: The semiconstrained distal radioulnar joint (DRUJ) prosthesis (Aptis Medical, Louisville, KY) is an option in the treatment of refractory DRUJ pathology that has shown promising results.
Purpose: This study aims to evaluate the short- to midterm functional outcomes, complications, and factors affecting outcomes of semiconstrained DRUJ arthroplasty.
Methods: We retrospectively reviewed 47 DRUJ arthroplasty procedures (on 46 consecutive patients) from a single surgeon between 2010 and 2022. Demographic data, range of motion, grip strength, and patient-reported outcome measures (PROMs: Quick Disability of the Arm, Shoulder, and Hand; Patient-Rated Wrist Evaluation; modified Mayo Wrist Score [mMWS]; and visual analog scale [VAS] for pain) were collected. Complications were classified as major (requiring surgery) or minor (treated conservatively).
Results: The average follow-up was 3.37 years (standard deviation, 3.51; range, 0.04–15.62 years). A total of 30% of patients (n = 14) were followed for more than 5 years. Postoperatively, supination significantly increased and VAS pain scores improved significantly. Patients also experienced improved function in the operative wrist as assessed by the mMWS. Patients with prior DRUJ and/or triangular fibrocartilage complex surgery were more likely to have additional diagnoses at the time of definitive surgery and our postoperative outcomes varied based on prior surgery and additional diagnoses, with a trend toward higher pain levels and worse PROMs. The overall complication rate was 17%, with one major complication consisting of a posttraumatic periprosthetic ulna fracture. All other complications were minor. Implant survival was 100%.
Conclusion: Semiconstrained DRUJ arthroplasty significantly improves pain levels and functional outcomes at short- to midterm follow-up. Patients who had undergone previous related surgeries were showed a trend toward higher postoperative pain levels and worse PROMs. Although complications were common, overall patient satisfaction and implant survivorship were high.
Level of Evidence: IV
Climate extreme triggers cold-water community rescue
Authors: Ebner B. C. , Lobegeiger J. , Coe J. , Balcombe S. , Latimer D. , Pickering G. , Marshall J. C. 
Published: October 2024

Context Mountain-top associated instream fauna with restricted ranges and limited dispersal capability are especially vulnerable to extinction under global warming and climate extremes. Aims Rescue and housing of multiple cold-water taxa on short timelines in reaction to extreme drought. Methods We undertook multi-species rescue (fishes: Gadopsis marmorata, n = 50; Galaxias olidus, n = 150; and a crayfish Euastacus sulcatus, n = 50) from the headwaters of the Condamine–Balonne catchment, temporarily holding animals in small (200 L) and large (1000 L) aquaria in single- and mixed-species contexts, at below 23°C. Key results Galaxias olidus was successfully kept in one of the small aquaria, but did not survive in mixed-species aquaria, partly being due to predation by other species. Euastacus sulcatus showed decreased survivorship at moulting (predation). Large Gadopsis marmorata (>100-mm total length, TL) was aggressive, whereas maintaining smaller individuals and using large aquaria served to dampen overall aggression levels. Conclusions Holding mixed taxa following field rescues requires attention to detail, including developmental-stage combinations within and across species, enclosure volume and availability of structure. Implications The simultaneous rescue of multiple co-occurring endemic taxa represents a promising aspect of research and adaptive management in the era of global climate change.