bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salvage of Necrotic-Appearing Limb after In Utero Endoscopic Lysis of Constriction Bands
Authors: Chon A. , Chen B. , Burkhalter W. , Chmait R.H. , Abdel-Sattar M. 
Published: April 2017

AbstractWe report a case of amniotic band syndrome complicated by constriction bands and marked distal swelling of both lower extremities. Color Doppler interrogation of the right lower extremity revealed complete lack of blood flow below the level of the constriction. Upon fetoscopic survey, the right lower extremity beyond the constriction band appeared dusky red and discolored with desquamation, consistent with a necrotic appearance. The constriction bands were cut in utero using endoshears, thereby allowing restoration of blood flow on postoperative day 1. The patient was counseled extensively regarding the possibility of limb dysfunction or amputation. However, the baby was born with functional lower extremities, and at 21 months of age, the child was cruising and jumping on his own. This case demonstrates that there is unique plasticity in fetal limb recovery after a severe ischemic injury that is not otherwise seen in postnatal life. Reperfusion of the necrotic-appearing limb resulted in restoration of appearance and function without apparent deleterious effects on the fetus. We believe the favorable outcome in this case was likely due to timeliness of the in utero lysis of amniotic bands and the plasticity of fetal healing.
Integrated Multimaneuver Dissection Technique of the Sylvian Fissure: Operative Nuances
Authors: Hafez Ahmad , Buçard João B , Tanikawa Rukoya 
Published: April 2017

Abstract

BACKGROUND
Several intracranial pathologies require opening the Sylvian fissure to be approached, and they are traditionally accessed through the frontal side of the superficial Sylvian veins. The integrated multimaneuver dissecting technique (IMMDT) interchanges the steps with the veins still secured for safe dissection, but the Sylvian fissure is approached from the optimal, safe side with integrated techniques.

OBJECTIVE
To determine whether the 1-way dissection of the Sylvian fissure converts to the more favorable and flexible IMMDT.

METHODS
The IMMDT for the Sylvian fissure was used on all our patients. The selected subjects (n = 3) had different pathologies, radiological data, and pre- and postoperative clinical data. These subjects were reviewed retrospectively.

RESULTS
The 3 patients presented with different pathologies necessitating opening the Sylvian fissure. The Sylvian fissure was not simple in all cases. In all 3 cases, the Sylvian veins and their branches were saved, as confirmed by postoperative images. The patients all had good neurological outcomes, with either stable or improved modified Rankin Scale at the last follow-up.

CONCLUSION
This study demonstrated that the IMMDT is a safe alternative to other ways of opening a Sylvian fissure composed of complex venous anatomy. The IMMDT aligned the fissure in multiple directions to the exposed trajectory, optimized its approach for a flexible, safe dissection, allowed the instruments to work into the Sylvian fissure, and facilitated exposure of the deep part of the fissure for the final dissection, all without scarifying any structure.
Trauma Collaborative Care Intervention: Effect on Surgeon Confidence in Managing Psychosocial Complications After Orthopaedic Trauma
Authors: Wegener Stephen T. , Carroll Eben A. , Gary Joshua L. , McKinley Todd O. , O'Toole Robert V. , Sietsema Debra L. , Castillo Renan C. , Frey Katherine P. , Scharfstein Daniel O. , Huang Yanjie , Collins Susan C. J. , MacKenzie Ellen J. ,   The Major Extremity Trauma Research Consortium (METRC)
Published: April 2017

Objectives:
The impact of the Trauma Collaborative Care (TCC) program on surgeon confidence in managing the psychosocial sequelae of orthopaedic trauma was evaluated as part of a larger prospective, multisite, cluster clinical trial. We compared confidence and perceived resource availability among surgeons practicing in trauma centers that implemented the TCC program with orthopaedic trauma surgeons in similar trauma centers that did not implement the TCC.

Design:
Prospective cohort design.

Setting:
Level-I trauma centers.

Participants:
Attending surgeons and fellows (N = 95 Pre and N = 82 Post).

Main Outcome Measurements:
Self-report 10-item measure of surgeon confidence in managing psychosocial issues associated with trauma and perceived availability of support resources.

Results:
Analyses, performed on the entire sample and repeated on the subset of 52 surgeons who responded to the survey at both times points, found surgeons at intervention sites experienced a significantly greater positive improvement (P < 0.05) in their (1) belief that they have strategies to help orthopaedic trauma patients change their psychosocial situation; (2) confidence in making appropriate referrals for orthopaedic trauma patients with psychosocial problems; and (3) belief that they have access to information to guide the management of psychosocial issues related to recovery.

Conclusions:
Initial data suggest that the establishment of the TCC program can improve surgeons' perceived availability of resources and their confidence in managing the psychosocial sequelae after injury. Further studies will be required to determine if this translates into beneficial patient effects.

Level of Evidence:
Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
Intertrochanteric Osteotomy for Femoral Neck Nonunion: Does “Undercorrection” Result in an Acceptable Rate of Femoral Neck Union?
Authors: Yuan Brandon J. , Shearer David W. , Barei David P. , Nork Sean E. 
Published: April 2017

Objectives:
To analyze the radiographic outcomes of intertrochanteric osteotomy for the treatment of femoral neck nonunion with “undercorrection” of the Pauwels angle and relative preservation of the proximal femoral anatomy.

Design:
Retrospective cohort study.

Setting:
Level-1 trauma center.

Patients:
Thirty-two patients with established femoral neck nonunions that had been treated with intertrochanteric osteotomy were retrospectively identified through Current Procedural Terminology codes. Seven patients were treated with 30 degree closing wedge osteotomy and 25 with a 20 degree or smaller osteotomy.

Intervention:
Valgus-producing intertrochanteric osteotomy with a blade plate.

Main Outcome Measurements:
Femoral neck and intertrochanteric osteotomy osseous union.

Results:
Thirty-one of 32 patients (97%) went on to osseous union of the femoral neck and all intertrochanteric osteotomies healed. There was no significant difference in the rate of union of the femoral neck between those patients treated with 30 versus 20 degree or less osteotomies. After osteotomy, the mean Pauwels angle decreased from 71 degrees (range 52–95 degrees) to 47 degrees (range 23–67 degrees) and the mean proximal femoral offset decreased by 11 mm (range 0–23 mm). Seven patients developed radiographic signs of avascular necrosis after osteotomy (22%). Three patients of these patients were converted to total hip arthroplasty (9%). Patients treated with a 30 degree osteotomy were more likely to develop avascular necrosis (67% vs. 12%, P-value = 0.014).

Conclusions:
Valgus-producing intertrochanteric osteotomy with a smaller degree of correction than has been traditionally described leads to an excellent rate of radiographic union while preserving more of the native proximal femoral anatomy.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
A National Survey of Neonatologists
Authors: Mirza Hussnain S. , Logsdon Gregory , Pulickal Anoop , Stephens Mark , Wadhawan Rajan 
Published: April 2017

Abstract
Point-of-care (POC) ultrasound refers to the use of portable imaging. Although POC ultrasound is widely available to the neonatologists in Australia and Europe, neonatologists in the United States report limited availability. Our objective was to seek the US neonatologists' perception of barriers and prerequisites in adopting POC ultrasound in neonatal intensive care units. An online survey link was sent via e-mail to 3000 neonatologists included in the database maintained by the American Academy of Pediatrics. Survey results (n = 574) were reported as percentage of total responses. Personal experience requiring an urgent sonography in managing cardiac tamponade or pleural effusion was reported by 78% respondents. However, emergent ultrasound (≤10 min) was not available in 80% of the neonatal intensive care units. We compared the responses based on years of clinical experience (>20 vs <20 years), with 272 (48%) neonatologist reporting more than 20 years of experience. Similarly, results from neonatal fellowship programs were compared with nonteaching/teaching hospitals, with 288 (50%) replies from neonatology fellowship programs. Compared with senior neonatologists, respondents with less than 20 years of clinical experience consider POC ultrasound enhances safety and accuracy of clinical procedures (87% vs 82%) and favor adopting POC ultrasound in clinical practice (92% vs 84%). There were no differences in opinion from neonatology fellowship programs compared with the nonteaching/teaching hospitals. Lack of training guidelines, inadequate support from local radiology department, and legal concerns were reported as the top 3 primary barriers in adopting POC ultrasound. If these barriers could be resolved, 89% respondents were inclined to adopt POC ultrasound in clinical practice.
Long-term memory in the migration movements of enucleatedAmoeba proteus
Authors: Bringas Carlos , Malaina Iker , Pérez-Samartín Alberto , Boyano María Dolores , Fedetz María , Pérez-Yarza Gorka , Cortes Jesus M. , De la Fuente Ildefonso M. 
Published: April 2017

AbstractHow motile, free unicellular organisms maximize the rate at which they encounter resources and develop optimal search strategies remains largely unknown. In fact, cell foraging is a very complex activity in which unicellular organisms integrate a diversity of external cues and develop efficient systemic movements to localize nourishment. These foraging strategies are critical when cells face conditions of scarce resources or they don’t possess information on where food is located. Here, in order to determine whether nuclear activity is directly involved in cell migration, we placed single, well-isolated, enucleated and non-enucleated starvedAmoeba proteuson nutrient-free petri dishes, and we then analyzed their trajectories of movement using non-linear dynamic tools. We found that despite being enucleated, the systemic responses of the protoplasm exhibited typical biological behaviors, moving with apparent normality, creeping along the substrate, developing pseudopodia and gobbling up prey. Our quantitative studies show that both the non-enucleated and enucleated amoebas display a similar migration structure, characterized by super-diffusivity, non-trivial long-term correlations and move-step fluctuations with scale invariant properties. In conclusion, the nuclear activity does not seem to directly control the systemic cellular movements involved in locating sparse resources.
SARNAclust: Semi-automatic detection of RNA protein binding motifs from immunoprecipitation data
Authors: Dotu Ivan , Adamson Scott , Coleman Benjamin , Fournier Cyril , Ricart-Altimiras Emma , Eyras Eduardo , Chuang Jeffrey H. 
Published: April 2017

AbstractRNA-protein binding is critical to gene regulation, controlling fundamental processes including splicing, translation, localization and stability, and aberrant RNA-protein interactions are known to play a role in a wide variety of diseases. However, molecular understanding of RNA-protein interactions remains limited, and in particular identification of the RNA motifs that bind proteins has long been a difficult problem. To address this challenge, we have developed a novel semi-automatic algorithm, SARNAclust, to computationally identify combined structure/sequence motifs from immunoprecipitation data. SARNAclust is, to our knowledge, the first unsupervised method that can identify RNA motifs at full structural resolution while also being able to simultaneously deconvolve multiple motifs. SARNAclust makes use of a graph kernel to evaluate similarity between sequence/structure objects, and provides the ability to isolate the impact of specific features through the bulge graph formalism. SARNAclust includes a key method for predicting RNA secondary structure at CLIP peaks, RNApeakFold, which we have verified to be effective on synthetic motif data. We applied SARNAclust to 30 ENCODE eCLIP datasets, identifying known motifs and novel predictions. Notably, we predicted a new motif for the protein ILF3 similar to that for the splicing factor hnRNPC, providing evidence for interaction between these two proteins. To validate our predictions, we performed a directed RNA bind-n-seq assay for two proteins: ILF3 and SLBP, in each case revealing the effectiveness of SARNAclust in predicting RNA sequence and structure elements important to protein binding. Availability:https://github.com/idotu/SARNAclust
Sex specific molecular responses of Quick-To-Court in Indian malarial vectorAnopheles culicifacies: conflict of mating and/or blood feeding?
Authors: De Tanwee Das , Sharma Punita , Rawal Charu , Kumari Seena , Tavetiya Sanjay , Yadav Jyoti , Hasija Yasha , Dixit Rajnikant 
Published: April 2017

AbstractUnderstanding the molecular basis of mosquito behavioral complexity is central to the design of novel molecular tool to fight against their vector borne diseases. Although, olfactory system play important role to guide and manage many behavioral co-ordinates including feeding, mating, breeding etc., but the sex specific regulation of olfactory responses remains unanswered. From our ongoing transcriptomic data annotation of blood fed adult female olfactory tissue ofA. culicifaciesmosquito, we identified a 383 bp long unique transcript encodingDrosophilahomolog of Quick-To-Court protein, previously shown to regulate the courtship behavior in adult maleDrosophila. A comprehensivein silicoanalysis predictsAc-qtcis 1536 bp long single copy gene encoding 511 AA long protein, having high degree of conservation with other insect homolog. Age dependent increased expression of putativeAc-qtcin the naïve mosquitoes correlates the maturation of olfactory system, necessary to meet sex specific conflicting demand of mating (mate finding) vs. host-seeking behavioral responses. Though, 16-18 hour of starvation did not alteredAc-qtcexpression in both the sexes, however blood feeding significantly modulated its response in the adult female mosquitoes, confirming that it may not be involved in sugar feeding associated behavioural regulation. Finally, a behavioural-cum-molecular assay indicated that natural dysregulation ofAc-qtcin late evening may promotes key mating event of successful insemination process. We hypothesize thatAc-qtcmay play unique role to meet and manage the sex specific conflicting demand of mosquito courtship behaviour and/or blood feeding behaviour in the adult female mosquitoes. A molecular mechanism elucidation may provide new knowledge to considerAc-qtcas a key molecular target for mosquito borne disease management.
Spatial heterogeneity and evolutionary dynamics modulate time to recurrence in continuous and adaptive cancer therapies
Authors: Gallaher Jill A. , Enriquez-Navas Pedro M. , Luddy Kimberly A. , Gatenby Robert A. , Anderson Alexander R. A. 
Published: April 2017

AbstractTreatment of advanced cancers has benefited from new agents that supplement or bypass conventional therapies. However, even effective therapies fail as cancer cells deploy a wide range of resistance strategies. We propose that evolutionary dynamics ultimately determine survival and proliferation of resistant cells, therefore evolutionary strategies should be used with conventional therapies to delay or prevent resistance. Using an agent-based framework to model spatial competition among sensitive and resistant populations, we apply anti-proliferative drug treatments to varying ratios of sensitive and resistant cells. We compare a continuous maximum tolerated dose schedule with an adaptive schedule aimed at tumor control through competition between sensitive and resistant cells. We find that continuous treatment cures mostly sensitive tumors, but with any resistant cells, recurrence is inevitable. We identify two adaptive strategies that control heterogeneous tumors: dose modulation controls most tumors with less drug, while a more vacation-oriented schedule can control more invasive tumors.
Power and shift invariant detection of dynamically coupled networks (PSIICOS) from non-invasive MEG data
Authors: Ossadtchi A. , Altukhov D. , Jerbi K. 
Published: April 2017

AbstractMEG reflects electrical activity of neuronal assemblies that coalesce and decoalesce in time providing for massively parallel and dynamic flow of information exchange in the brain. There is a growing evidence that our behavior is mediated by simultaneous activity of several interacting and simultaneously active cortical networks. Not only the location of network nodes but also the temporal profiles of such interaction are of interest. The existing methods are primarily concerned with proposing novel measures of synchrony to be applied to the cortical region activity signals extracted from the MEG data using beamforming or other spatial filtering approaches.In this work we propose to render the task of estimating network topology and temporal profiles as a source estimation problem but in the space of interacting topographies. The proposed point of view has a number of benefits as it allows to use the wealth of techniques and intuition accumulated in the community when dealing with source estimation problems. Operating in the interacting topographies space we first propose subspace projection method to significantly reduce the spatial leakage effect in the cross-spectral matrix. We then use subspace matching metrics to extract a set of networks with their synchronicity profiles that explain the variance in the sensor space cross-spectral matrix, much like regular dipoles and their activations explain the variance in the regular evoked responses.The preliminary results of application of this method to a simple self-paced voluntary finger movement paradigm comparing allowed to observe functional coupling between primary motor and supplementary motor area and establish that this coupling can be characterized with near-zero phase delay between the oscillatory activity of these two regions. This observed synchrony pattern is physiologically plausible and is in agreement with the current hypothesis about the neural mechanics of the motor acts.
Geographic range dynamics drove ancient hybridization in a lineage of angiosperms
Authors: Folk R.A. , Visger C.J. , Soltis P.S. , Soltis D.E. , Guralnick R.P. 
Published: April 2017

AbstractFactors explaining global distribution patterns have been central to biology since the 19th century, yet failure to combine dispersal-based biogeography with shifts in habitat suitability remains a present-day setback in understanding geographic distributions present and past, and time-extended trajectories of lineages. The lack of methods in a suitable integrative framework stands as a conspicuous shortcoming for reconstructing these dynamics. Here we showcase novel methods to overcome these methodological gaps, broadening the prospects for phyloclimatic modeling. We focus on a clade in the angiosperm genusHeucheraendemic to southern California that experienced ancient introgression from circumboreally distributed species ofMitella,testing hypotheses regarding biotic contact in the past between ancestral species lacking a fossil record. We obtain strong support for a past contact zone in northwestern North America, resolving this paradox of hybridization between ancestors of taxa currently separated by ∼1300 km.
Effect of main controlling factor on the corrosion behaviour of API X65 pipeline steel in the CO2/oil/water environment
Authors: Cheng Yuanpeng , Li Zili , Zhao Yalei , Xu Yazhou , Liu Qianqian , Bai Yu 
Published: April 2017

Purpose
The purpose of this paper was to investigate the corrosion behaviour of API X65 pipeline steel in the simulated CO2/oil/water emulsion using weight loss technique, potentiodynamic polarization technique and characterization of the corroded surface techniques.

Design/methodology/approach
The weight loss analysis, electrochemical study and surface investigation were carried out on API X65 pipeline steel that had been immersed in the CO2/oil/water corrosive medium to understand the corrosion behaviour of gathering pipeline steel. The weight loss tests were carried out in a 3L autoclave, and effects of temperature, CO2 partial pressure, water cut and flow velocity on the CO2 corrosion rate of API X65 pipeline steel were studied. Electrochemical studies were carried out in a three-electrode electrochemical cell with the test temperature was 60°C, and the CO2 partial pressure was 1 atm by recording open circuit potential/time and potentiodynamic polarization characteristics. The surface and cross-sectional morphologies of corrosion product scales were characterized using scanning electron microscopy. The phases of corrosion product scales were investigated using X-ray diffraction.

Findings
The results showed that water cut was the main controlling factor of API X65 steel corrosion under the conditions of CO2/oil/water multiphase flow, and it had significant impact on corrosion morphology. In the case of higher water cut or pure water phase, general corrosion occurred on the steel surface. While water cut was below 70 per cent, corrosion morphology transformed into localized corrosion, crude oil decreased corrosion rate significantly and played a role of inhibitor. Crude oil hindered the corrosion scales from being dissolved by corrosive medium and changed dimension and accumulation pattern of the crystal grain, thickness and structure of the corrosion scales; thus, it influenced the corrosion rate. The primary corrosion product of API X65 steel was ferrous carbonate, which could act as a protective film at low water cut so that the corrosion rate can be reduced.

Originality/value
The results can be helpful in selecting the suitable corrosion inhibitors and targeted anti-corrosion measures for CO2/oil/water corrosive environment.