bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Jumping on the Blood Pressure Bandwagon”: Nurse, GP, and Patient Perspectives of a General Practice Nurse‐Led Hypertension Management Intervention
Authors: Stephen Catherine , Zwar Nick , Batterham Marijka , Halcomb Elizabeth 
Published: October 2024

ABSTRACTTo explore the perceptions and experiences of general practice nurses, general practitioners, and patients who participated in a nurse‐led intervention to improve blood pressure control. Given the impact of hypertension on rates of premature death and disability, it is important that interventions be evaluated to reduce blood pressure. A key component of such evaluation is understanding the experiences of participants and clinicians. Understanding these experiences can provide insight into acceptability and feasibility that informs future research and implementation. Qualitative descriptive study within a mixed methods project. Semi‐structured interviews were conducted post‐intervention with six patients, five nurses, and three general practitioners. Interviews were audio‐recorded, transcribed, and analyzed using thematic analysis. The COREQ checklist guided reporting. Three themes around the need for change, navigating change, and sustaining change were revealed. In highlighting the need for change, participants recognized that it was time to actively work toward improved blood pressure control. In navigating change, general practice nurses were perceived as ideally placed to communicate risks around uncontrolled blood pressure and support lifestyle change. The final theme, sustaining change revealed the feasibility of the intervention in practice, however, clinician participants identified that appropriate funding is required to ensure sustainability. Nurse‐led intervention to improve blood pressure control in general practice is feasible in practice and acceptable to patients. This highlights an opportunity for nurses to play a more proactive role in hypertension management within general practice. To ensure sustainability, however, issues such as funding, teamwork, and collaboration need to be addressed.Trial Registration: Australian and New Zealand Clinical Trials Registry: ACTRN12618000169246
The itinerary of children in search of healthcare: A scoping review and proposal of an explanatory model
Authors: Cristino Joseir Saturnino , de Farias Altair Seabra , de Melo Lilian Dornelles Santana , Machado Vinícius Azevedo , Sachett Jacqueline , Monteiro Wuelton 
Published: October 2024

AbstractThis scoping review mapped the academic literature focused on the therapeutic itinerary of children who seek care in health services and proposed an explanatory model to expand the concept and classification of these health itineraries. A total of 789 articles were reviewed, of which 28 were eligible for inclusion. In these 28 it was possible to observe that the child's therapeutic itinerary is more than a physical path, but also encompasses all choices within a specific social and cultural environment in which the child is inserted. Our proposal is to expand the concept beyond the therapeutic, classifying the itinerary also according to the objective, the decision‐making agent, respect for the presence of company, the health subsystem used, according to the physical continuity of the itinerary, the perception of efficacy of the patient, the nature of the illness, the administration of healthcare, the means of transport used, the person providing information about the itinerary, the planning of the itinerary and its completeness. Knowing the child's itineraries toward healthcare allows the development of innovative discourses and practices for future public policies, through which the principles of comprehensiveness and resoluteness in children's health would be strengthened. There is still a need to deepen knowledge about the meanings and feelings regarding their interpretations of the events suffered in childhood.
Phosphate promotes Arabidopsis root skewing and circumnutation through reorganisation of the microtubule cytoskeleton
Authors: Sheng Hui , Bouwmeester Harro J. , Munnik Teun 
Published: October 2024

Summary
Phosphate (Pi) plays a key role in plant growth and development. Hence, plants display a range of adaptations to acquire it, including changes in root system architecture (RSA). Whether Pi triggers directional root growth is unknown.
We investigated whether Arabidopsis roots sense Pi and grow towards it, that is whether they exhibit phosphotropism. While roots did exhibit a clear Pi‐specific directional growth response, it was, however, always to the left, independent of the direction of the Pi gradient.
We discovered that increasing concentrations of KH2PO4, trigger a dose‐dependent skewing response, in both primary and lateral roots. This phenomenon is Pi‐specific – other nutrients do not trigger this – and involves the reorganisation of the microtubule cytoskeleton in epidermal cells of the root elongation zone. Higher Pi levels promote left‐handed cell file rotation that results in right‐handed, clockwise, root growth and leftward skewing as a result of the helical movement of roots (circumnutation).
Our results shed new light on the role of Pi in root growth, and may provide novel insights for crop breeding to optimise RSA and P‐use efficiency.
Reconciling water‐use efficiency estimates from carbon isotope discrimination of leaf biomass and tree rings: nonphotosynthetic fractionation matters
Authors: Yu Yong Zhi , Ma Wei Ting , Wang Xuming , Tcherkez Guillaume , Schnyder Hans , Gong Xiao Ying 
Published: October 2024

Summary
Carbon isotope discrimination (∆) in leaf biomass (∆BL) and tree rings (∆TR) provides important proxies for plant responses to climate change, specifically in terms of intrinsic water‐use efficiency (iWUE). However, the nonphotosynthetic 12C/13C fractionation in plant tissues has rarely been quantified and its influence on iWUE estimation remains uncertain.
We derived a comprehensive, ∆ based iWUE model (iWUEcom) which includes nonphotosynthetic fractionations (d) and characterized tissue‐specific d‐values based on global compilations of data of ∆BL, ∆TR and real‐time ∆ in leaf photosynthesis (∆online). iWUEcom was further validated with independent datasets.
∆BL was larger than ∆online by 2.53‰, while ∆BL and ∆TR showed a mean offset of 2.76‰, indicating that ∆TR is quantitatively very similar to ∆online. Applying the tissue‐specific d‐values (dBL = 2.5‰, dTR = 0‰), iWUE estimated from ∆BL aligned well with those estimated from ∆TR or gas exchange. ∆BL and ∆TR showed a consistent iWUE trend with an average CO2 sensitivity of 0.15 ppm ppm−1 during 1975–2015.
Accounting for nonphotosynthetic fractionations improves the estimation of iWUE based on isotope records in leaf biomass and tree rings, which is ultimate for inferring changes in carbon and water cycles under historical and future climate.
Providing individualized services under complex conditions: A configurational analysis of street‐level organizations
Authors: Andreassen Tone Alm , Breit Eric , Saltkjel Therese 
Published: October 2024

AbstractIndividualized services are provided under complex conditions, as a variety of factors can affect the ability of a street‐level organization to adapt its services to individual needs and circumstances. Especially challenging are tensions between the means of control and standardization following new public management (NPM) and post‐NPM ideas of holistic and coordinated services. Through a fuzzy‐set qualitative comparative analysis of Norwegian sector‐spanning street‐level organizations, we show three different configurations that can promote individualized services. These consist of variations of structural circumstances (size, service variety); organizational responses (goal coherence, cross working); and manager capacity (professional background, managerial orientation). Service individualization is not an outcome of the interaction between street‐level workers and clients alone, but an outcome of street‐level organizations and their managers' use of measures and competencies across service sectors, and of their capacity to develop a shared perception of goals and an organization that handles institutional complexity.
Maize‐Tripsacum‐Teosinte allopolyploid (MTP), a novel dwarf mutant inducer tool in maize
Authors: Zhou Yang , Li Yang , Luo Lin , Zhang Delong , Wang Xingyu , Chen Yu , Zhang Yibo , Zhang Qiyuan , Luo Hanyu , Xie Pengfei , Du Yiyang , Duan Saifei , Zhou Yong , Yang Tao , Li Xiaofeng , He Ruyu , Li Yingzheng , Cheng Mingjun , Li Yan , Ma Zhibin , He Jianmei , Rong Tingzhao , Tang Qilin 
Published: October 2024

SummaryDwarf plant architecture facilitates dense planting, and increased planting densities boost the maize yield. However, breeding applications of dwarfing materials for maize are currently limited. There is an urgent need remove the obstacles to applying dwarf resources. Here, we innovated a new method to add a novel maize dwarf germplasm through the distant hybridization of Maize–Tripsacum–Teosinte allopolyploid (MTP) with maize. We identified ten independent dwarf families with unique characteristics. Five germplasms in our library were controlled by their respective dwarf genes. However, no allele was controlled by Br2. Subsequently, d024 in the library was successfully fine mapped, revealing its linkage to indel‐4 in ZmCYP90D1. The indel‐4 polymorphism regulates the expression of ZmCYP90D1 and is controlled by an upstream transcription factor (ZmBES1/BZR1‐5). The indel‐4 of ZmCYP90D1 allele, which reduces plant height, originated from Tripsacum, a wild variety of maize. However, d024 exhibits sensitivity to brassinosteroids (BRs), with lower castasterone levels in the internodes than that in the wild type. Furthermore, ZmCYP90D1 interacted with ZmFDXs and ZmNAD(P)H to positively regulate the downstream BR synthesis pathway. Additionally, we showed that introgressing the indel‐4 of the Tripsacum allele into modern hybrids ensures yield potential and improves the harvest index under high‐density conditions. Overall, as we begin to manufacture highly engineered dwarf materials using the MTP, this approach will solve the problems faced by corn dwarfs.
Allelic Variation of BnaFTA2 and BnaFTC6 Is Associated With Flowering Time and Seasonal Crop Type in Rapeseed (Brassica napus L.)
Authors: Wan Ming , Zhao Dawei , Lin Shengzhe , Wang Pengfei , Liang Baoling , Jin Qingdong , Jiao Yushun , Song Yixian , Ge Xianhong , King Graham J. , Yang Guangsheng , Wang Jing , Hong Dengfeng 
Published: October 2024

ABSTRACTDifferent ecological types of rapeseed (Brassica napus L.), including winter, spring, and semi‐winter cultivars, exhibit varying flowering times and cannot be planted in the same cultivation areas. FLOWERING LOCUS T (FT) plays a key role in regulating flowering. In allotetraploid B. napus six copies of FT (BnaFT) have been reported. However, there is uncertainty about how the translated products of each paralog, as well as cis‐allelic variations at each locus, contribute functionally to flowering time and define specific crop types. In this study, we confirm that BnaFT exhibit distinct expression patterns in different crop types of rapeseed. Using the CRISPR/Cas9 gene editing system, we provide functional evidence that the mutants between Bnaft paralogues affects the regulation of flowering time. Furthermore, we identify a new haplotype of BnaFT.A2 that is associated with early flowering time, although this appears necessary but not sufficient to confer a spring type phenotype. Three haplotypes of BnaFT.C6 were further identified and associated with both flowering time and crop types. We speculate that variations in both BnaFT.A2 and BnaFT.C6 may have undergone diversifying selection during the divergence of seasonal crop types in rapeseed.
A Next‐Generation Combinatorial Genomic Strategy Scans Genomic Loci Governing Heat Stress Tolerance in Chickpea
Authors: Mohanty Jitendra K. , Yadav Antima , Narnoliya Laxmi , Thakro Virevol , Nayyar Harsh , Dixit Girish P. , Jha Uday Chand , Vara Prasad P. V. , Agarwal Pinky , Parida Swarup K. 
Published: October 2024

ABSTRACTIn the wake of rising earth temperature, chickpea crop production is haunted by the productivity crisis. Chickpea, a cool season legume manifests tolerance in several agro‐physiological level, which is complex quantitative in nature, and regulated by multiple genes and genetic networks. Understanding the molecular genetic basis of this tolerance and identifying key regulators can leverage chickpea breeding against heat stress. This study employed a genomics‐assisted breeding strategy utilizing multi‐locus GWAS to identify 10 key genomic regions linked to traits contributing to heat stress tolerance in chickpea. These loci subsequently delineated few key candidates and hub regulatory genes, such as RAD23b, CIPK25, AAE19, CK1 and WRKY40, through integrated genomics, transcriptomics and interactive analyses. The differential transcript accumulation of these identified candidates in contrasting chickpea accessions suggests their potential role in heat stress tolerance. Differential ROS accumulation along with their scavengers' transcript abundance aligning with the expression of identified candidates in the contrasting chickpea accessions persuade their regulatory significance. Additionally, their functional significance is ascertained by heterologous expression and subsequent heat stress screening. The high confidence genomic loci and the superior genes and natural alleles delineated here has great potential for swift genomic interventions to enhance heat resilience and yield stability in chickpea.
Factors affecting development of pseudothecia produced by Venturia effusa in vitro
Authors: Charlton Nikki D. , Bock Clive H. , Boykin Debbie , Young Carolyn A. 
Published: October 2024

AbstractPecan scab, caused by Venturia effusa, is the most destructive disease of pecan in the south‐eastern United States. The fungus was assumed to perpetuate solely through asexually produced conidia. Yet the identification of heterothallic mating type idiomorphs has led to successful crosses producing pseudothecia in vitro. To investigate factors influencing the development of pseudothecia, three isolates of each mating type (MAT1‐1 × MAT1‐2) were crossed pairwise on oatmeal agar. Pseudothecial production and maturation were assessed at 4, 8, 12, 16 and 22°C, with incubation periods of 2, 3 or 4 months, either with or without light (0 or 12 h photoperiod) during maturation. There were significant effects of temperature, month and cross on the production of pseudothecia. Similar numbers of pseudothecia were produced at 4, 8, 12 and 16°C (1.8–2.3 pseudothecia), but significantly fewer at 22°C (0.1 pseudothecia). Pseudothecia remained immature at 22°C suggesting a cold period is required for ascospore maturation. The isolate and mating type cross affected the number of pseudothecia, with both MAT1‐1 and MAT1‐2 contributing to the number produced, which interacted with time. MAT1‐1 had the greatest effect on the number of pseudothecia produced, but this was modulated by MAT2‐2. At the highest temperature there were no differences among mating type crosses. Light had no effect on maturation of pseudothecia in this study. The results indicate the likely temperatures and durations required to produce pseudothecia in the field; ascospores may play a role as primary inoculum in the disease cycle of pecan scab.
Parvovirus B19 rebound outbreak 2024 and implications for blood‐ and plasma‐product safety
Authors: Farcet Maria R. , Karbiener Michael , Aberham Claudia , Powers Nicholas , Aue Daniel , Kreil Thomas R. 
Published: October 2024

AbstractBackgroundSince the beginning of 2024, several European countries reported unusually high numbers of Human parvovirus B19 (B19V) infections. An increase in B19V incidence rate might have implications for blood products for direct transfusion, however, large data sets for analysis of this outbreak are missing.Study Design and MethodsB19V nucleic acid testing (NAT) of plasma donations collected between June 2018 and May 2024 from mainly Central European countries (n = 9.6 million) and the United States (n = 70.7 million) was done to the individual donation level.ResultsIn Central Europe, there was a marked increase in B19V incidence from November 2023 onwards, which peaked in April 2024 with a 33‐fold higher than average B19V incidence versus before the COVID‐19 pandemic. In the United States, a similar trend was seen, with a yet still 6‐fold lower increase than in Europe at the same time. The largest increase in B19V positivity was seen in the youngest plasma donor cohort.DiscussionA B19V infection gap during the COVID‐19 pandemic is likely the basis for the rebound outbreak in 2023/2024, particularly in Europe. B19V NAT of millions of plasma donations provides for large scale numbers to solidify available epidemiology insight, and to support adequate risk assessments. Based on the situation it may be prudent to consider B19V NAT for blood components specifically directed towards transfusion to higher risk recipients, or alternatively, preselecting B19V seropositive individuals or advanced age donors at higher likelihood of seropositivity and thus lower risk of virus transmission.
Ethnographic film as world‐making: Connecting visual anthropology with Science and Technology Studies
Authors: Verstappen Sanderien , Davies Sarah R. 
Published: October 2024

AbstractThis article initiates a dialogue between Science and Technology Studies (STS) and visual anthropology. It demonstrates how ethnographic filmmaking can be an avenue for STS research, teaching, and thinking. Highlighting similarities between knowledge practices in the natural sciences and the social sciences, it conceptualizes filmmaking as a world‐making project. This is a new answer to the persistent question of how to understand the use of film as a research method. Our argument is based on a collection of short films made during an interdisciplinary course which explored the potential of ethnographic filmmaking for investigating the day‐to‐day doings of science and technology.
Hamiltonian System-Based Symplectic Framework for Analytical Vibration Analysis of Microplates
Authors: Hu Zhaoyang , Li Rui 
Published: October 2024

Abstract
The classical elasticity is inadequate for the dynamic analysis of microplates due to the size effect. This study incorporates a higher-order strain gradient theory into the Hamiltonian system-based symplectic framework and derives new analytical solutions for the free vibration of microplates. The analytical solutions are obtained using rigorous mathematical techniques, including separation of variables, symplectic eigen expansion, and superposition, without relying on predetermined solution forms. Hence, they are not restricted to Lévy-type boundary conditions. Using these analytical solutions, we present comprehensive vibration results for microplates and perform detailed parametric studies to explore the impact of length scale parameters on the natural frequencies. Given the growing demand for microplates in advanced engineering applications, the obtained analytical solutions are expected to facilitate their design and performance optimization.
A Conceptual Design Method Based on Concept–Knowledge Theory and Large Language Models
Authors: Chen Liuqing , Xia Duowei , Jiang ZhaoJun , Tan Xinyang , Sun Lingyun , Zhang Lin 
Published: October 2024

Abstract
To obtain innovative concepts in the design, designers often need to retrieve and use interdisciplinary knowledge. Concept–knowledge (C–K) theory emphasizes the role of knowledge and introduces the knowledge (K) space and concept (C) space, employing operators to transform the contents between these spaces. Some studies, based on this theory, have successfully provided designers with different forms of knowledge to stimulate concept generation. However, the amount of knowledge provided in these studies is limited, and they fail to offer convenient methods for knowledge retrieval and reasoning, making it challenging to meet the needs of conceptual design across different fields. This paper proposes an enhanced C–K method leveraging large language models (LLMs) to help designers retrieve knowledge and uncover potentially new concepts. Our method redefines the C space and K space within the context of LLMs, dividing the properties of concept into function, appearance, and technology, and requiring the knowledge to correspond to these properties, thereby facilitating a structured connection between concepts and knowledge. Based on this definition, we achieved flexible knowledge retrieval and concept ideation leveraging LLMs. We also conducted a case study on wearable devices to validate our method. The results showed that our method helped designers to retrieve professional knowledge and inspired them to create feasible and innovative concepts.
The glutamate receptor antagonist ifenprodil inhibits hepatitis E virus infection
Authors: Klöhn Mara , Gömer André , He Qiyu , Brown Richard J. P. , Todt Daniel , Wang Lin , Steinmann Eike 
Published: October 2024

ABSTRACT

Infection with hepatitis E virus (HEV) represents a global problem, with over 20 million people infected annually. No specific antiviral drugs are available for treating HEV infection, necessitating the development of novel targeted therapeutics. Here, we report that the N-methyl-D-aspartate receptor (NMDAR) antagonist ifenprodil, a clinically approved drug used to treat idiopathic pulmonary fibrosis (IPF), is an HEV inhibitor in liver-derived cells.
In vitro
investigation demonstrates that ifenprodil suppresses viral protein expression in a dose-dependent manner in human hepatoma cells by inhibiting early stages of viral infection. We also found that ifenprodil modulates host cell intrinsic biological processes distinct from virus-induced innate immunity, inhibiting HEV RNA accumulation in primary human hepatocytes. Finally, the inhibitory effect of ifenprodil
in vivo
was also tested in rabbits challenged with the HEV-3ra CHN-BJ-R14 strain. Fecal virus shedding was below the limit of detection in two animals for both ribavirin-treated and ifenprodil-treated rabbits compared to vehicle-treated control animals. Our data demonstrate that ifenprodil is an effective anti-HEV compound with potential as a therapeutic candidate for the treatment of HEV infection.