bluuebunny/crossref_metadata_2025_split huggingface dataset:
The antiallodynic action of pregabalin in neuropathic pain is independent from the opioid system
Authors: Kremer Mélanie , Yalcin Ipek , Nexon Laurent , Wurtz Xavier , Ceredig Rhian Alice , Daniel Dorothée , Hawkes Rachael Aredhel , Salvat Eric , Barrot Michel 
Published: March 2016

Background Clinical management of neuropathic pain, which is pain arising as a consequence of a lesion or a disease affecting the somatosensory system, partly relies on the use of anticonvulsant drugs such as gabapentinoids. Therapeutic action of gabapentinoids such as gabapentin and pregabalin, which act by the inhibition of calcium currents through interaction with the α2δ-1 subunit of voltage-dependent calcium channels, is well documented. However, some aspects of the downstream mechanisms are still to be uncovered. Using behavioral, genetic, and pharmacological approaches, we tested whether opioid receptors are necessary for the antiallodynic action of acute and/or long-term pregabalin treatment in the specific context of neuropathic pain. Results Using the cuff model of neuropathic pain in mice, we show that acute pregabalin administration at high dose has a transitory antiallodynic action, while prolonged oral pregabalin treatment leads to sustained antiallodynic action, consistent with clinical observations. We show that pregabalin remains fully effective in μ-opioid receptor, in δ-opioid receptor and in κ-opioid receptor deficient mice, either female or male, and its antiallodynic action is not affected by acute naloxone. Our work also shows that long-term pregabalin treatment suppresses tumor necrosis factor-α overproduction induced by sciatic nerve constriction in the lumbar dorsal root ganglia. Conclusions We demonstrate that neither acute nor long-term antiallodynic effect of pregabalin in a context of neuropathic pain is mediated by the endogenous opioid system, which differs from opioid treatment of pain and antidepressant treatment of neuropathic pain. Our data are also supportive of an impact of gabapentinoid treatment on the neuroimmune aspect of neuropathic pain.
A tool for reproducible research: From data analysis (in R) to a typeset laboratory notebook (as .pdf) using the text editor Emacs with the 'mp' package
Authors: Dardis Christopher , Woolf Eric C , Scheck Adrienne C 
Published: March 2016

SoftwareMuch scientific research makes use of commonly available 'office' software. While numerous more fully-featured open-source alternatives exist, the integration of diverse tools and platforms which their use often entails can be challenging. The mp package for Emacs aims to bring together a number of these elements with the goal of simplifying the process of converting an .R file, as used for data analysis, to a nicely formatted .pdf which includes the complete description of the methods and interpretation. We discuss the rationale for development of the package and illustrate its applications and options with a series of experiments from our laboratory.Experimental workWe demonstrate the inhibitory effects of the ketone body \emph{beta}-hydroxybutyrate (BHB) on the growth and motility of a cancer cell line. BHB is produced endogenously; levels may be increased in certain medial conditions e.g. diabetic ketoacidosis. They may also be raised voluntarily e.g. by adopting the ketogenic diet. BHB is known to inhibit the growth of other neoplastic cell lines. However the finding that it can do so in a cell line selected for their propensity to metastasize to the brain is novel. Given the challenges in treating patients with melanoma metastatic to brain, this work strengthens the rationale for investigating the ketogenic diet as a potential adjunct to treatment in such cases.
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle
Authors: Chaponnier Christine , Gabbiani Giulio 
Published: March 2016

Higher vertebrates express six different highly conserved actin isoforms that can be classified in three subgroups: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins (CYAs), β-CYA and γ-CYA. The variations among isoactins, in each subgroup, are due to 3-4 amino acid differences located in their acetylated N-decapeptide sequence. The first monoclonal antibody (mAb) against an actin isoform (α-SMA) was produced and characterized in our laboratory in 1986 (Skalli et al., 1986). We have further obtained mAbs against the 5 other isoforms. In this report, we focus on the mAb anti-α-SKA and anti-α-CAA obtained after immunization of mice with the respective acetylated N-terminal decapeptides using the Repetitive Immunizations at Multiple Sites Strategy (RIMMS). In addition to the identification of their epitope by immunoblotting, we describe the expression of the 2 sarcomeric actins in mature skeletal muscle and during muscle repair after micro-lesions. In particular, we analyze the expression of α-CAA, α-SKA and α-SMA by co-immunostaining in a time course frame during the muscle repair process. Our results indicate that a restricted myocyte population expresses α-CAA and suggest a high capacity of self-renewal in muscle cells. These antibodies may represent a helpful tool for the follow-up of muscle regeneration and pathological changes.
Interprofessional graduate programme for nursing-paramedicine degree graduates: work outcomes and participant experience
Authors: Considine Julie , Walker Tony 
Published: March 2016

The 2011/12 interprofessional graduate programme (IPG) was Australia's first programme for graduates of double degrees in nursing and paramedicine. Aims: The aim of this study was to examine the employment outcomes and participant experience of the first cohort of 10 IPG participants. Methods: Data were collected by repeated surveys (6 months prior to, 6 months and 18 months following IPG completion) and interviews. Results: There were 10 IPG participants, nine completed all surveys and nine agreed to be interviewed. Two were males and the average age was 23.7 years (SD=1.3). At IPG completion, all participants were employed by Ambulance Victoria: five participants were working casual shifts in emergency nursing. Themes related to career choice were that casual employment was easier in nursing and paramedicine had higher levels of freedom and more regular shift patterns. Mean scores for involvement decreased (p<0.018) and manager control increased (p<0.018). The four themes related to participant experience were: i) best of emergency health care; ii) knowledge and experience; iii) understanding and respect; and iv) chopping and changing. Conclusions: Participants' experience of IPG were mostly positive. Traditional professional and industrial structures still prevent double degree graduates using their skills and knowledge to their full capability.
Credits granted for professional education of paramedics and nurses at at US colleges with paired programmes
Authors: Phelps Scott 
Published: March 2016

Aims: To compare professional education requirements for Commission on Accreditation of Allied Health Educations Programs (CAAHEP)-accredited community college paramedic programs and associate degree registered nurse programmes at the same institution. Methods: All CAAHEP-accredited community college paramedic programmes were identified from the CAAHEP website. The college website was visited to determine credits granted for paramedic professional education, followed by a search of the college website to determine if there was an associate degree registered nursing programme. If there was, the credits granted for registered nurse professional education was determined and the pair was recorded. Results: At the 259 community colleges in the United States that had both a CAAHEP-accredited paramedic and registered nursing education programme that were offered for college credit, paramedic professional education was granted more college credit than nursing professional education at the individual college level 81% of the time. At the national level, median (48 versus 40 college credits) of paramedic professional education exceeded associate degree registered nursing professional education by 20%. Conclusions: Professional education for paramedics is granted more college credit that the professional ed-ucation for associate degree registered nurses.
Extracting Electronic Health Record Data in a Practice-Based Research Network:
Lessons Learned from Collaborations with Translational Researchers
Authors: Cole Allison M. , Stephens Kari A. , Keppel Gina A. , Estiri Hossein , Baldwin Laura-Mae 
Published: March 2016

Context: The widespread adoption of electronic health records (EHRs) offers
significant opportunities to conduct research with clinical data from patients outside
traditional academic research settings. Because EHRs are designed primarily for clinical
care and billing, significant challenges are inherent in the use of EHR data for
clinical and translational research. Efficient processes are needed for translational
researchers to overcome these challenges. The Data QUEST Coordinating Center (DQCC),
which oversees Data QUEST – a primary care EHR data sharing infrastructure – created
processes that that guide EHR data extraction for clinical and translational research
across these diverse practices. We describe these processes and their application in a
case example.Case Description: The DQCC process for developing EHR data extractions not
only supports researchers access to EHR data, but supports this access for the purpose
of answering scientific questions. This process requires complex coordination across
multiple domains, including: 1) understanding the context of EHR data; 2) creating and
maintaining a governance structure to support exchange of EHR data; and 3) defining data
parameters that are used in order to extract data from the EHR.1,2,3,4 We use the
Northwest-Alaska Pharmacogenomics Research Network (NWA-PGRN) as a case example that
focuses on pharmacogenomic discovery and clinical applications to describe the DQCC
process. The NWA-PGRN collaborates with Data QUEST to explore ways to leverage primary
care EHR data to support pharmacogenomics research.Findings: Preliminary analysis on the
case example shows that initial decisions about how researchers define the study
population can influence study outcomes.Major Themes and Conclusions: The experience of
the DQCC demonstrates that Coordinating Centers provide expertise in helping researchers
understand the context of EHR data, create and maintain governance structures, and guide
the definition of parameters for data extractions. This expertise is critical to support
research with EHR data. Replication of these strategies through Coordinating Centers may
lead to more efficient translational research. Investigators must also consider the
impact of initial decisions in defining study groups that may potentially affect
outcomes.
Protective Effects of Agmatine against Chlorpromazine- Induced Toxicity in the Liver of Wistar Rats
Authors: Dejanović Bratislav , Stevanović Ivana , Ninković Milica , Stojanović Ivana , Lavrnja Irena , Radičević Tatjana 
Published: March 2016

SummaryThe metabolic pathways of chlorpromazine (CPZ) toxicity were tracked by assessing oxidative/nitrosative stress markers. The main objective of the study was to test the hypothesis that agmatine (AGM) prevents oxidative/nitrosative stress in the liver of Wistar rats 15 days after administration of CPZ. All tested substances were administered intraperitoneally (i.p.) for 15 consecutive days. The rats were divided into four groups: the control group (C, 0.9 % saline solution), the CPZ group (CPZ, 38.7 mg/kg b.w.), the CPZ+AGM group (AGM, 75 mg/kg b.w. immediately after CPZ, 38.7 mg/kg b.w. i.p.) and the AGM group (AGM, 75 mg/kg b.w.).Rats were decapitated 15 days after the appropriate treatment. In the CPZ group, CPZ concentration was significantly increased compared to C values (p<0.01), while AGM treatment induced the significant decrease in CPZ concentration in the CPZ+AGM group (p<0.05) and the AGM group (p<0.01). CPZ application to healthy rats did not lead to any changes of lipid peroxidation in the liver compared to the C group, but AGM treatment decreased that parameter compared to the CPZ group (p<0.05). In CPZ liver homogenates, nitrite and nitrate concentrations were increased compared to controls (p<0.001), and AGM treatment diminished that parameter in the CPZ group (p<0.05), as well as in the AGM group (p<0.001). In CPZ animals, glutathione level and catalase activity were decreased in comparison with C values (p<0.01 respectively), but AGM treatment increased the activity of catalase in comparison with CPZ animals (p<0.05 respectively). Western blot analysis supported biochemical findings in all groups. Our results showed that treatment with AGM significantly supressed the oxidative/nitrosative stress parameters and restored antioxidant defense in rat liver.
Foaming and emulsifying properties of pectin isolated from different plant materials
Authors: Yancheva Nikoleta , Markova Daniela , Murdzheva Dilyana , Vasileva Ivelina , Slavov Anton 
Published: March 2016

Abstract
The foaming and emulsifying properties of pectins obtained from waste rose petals, citrus pressings, grapefruit peels and celery were studied. It was found that the highest foaming capacity showed pectin derived from celery. The effect of pectin concentration on the foaming capacity of pectin solutions was investigated. For all the investigated pectins increasing the concentration led to increase of the foaming capacity. Emulsifying activity and emulsion stability of model emulsion systems (50 % oil phase) with 0.6 % pectic solutions were determined. The highest emulsifying activity and stability showed pectin isolated by dilute acid extraction from waste rose petals.
Determination and discrimination of biodiesel fuels by gas chromatographic and chemometric methods
Authors: Milina R. , Mustafa Z. , Bojilov D. , Dagnon S. , Moskovkina M. 
Published: March 2016

Abstract
Pattern recognition method (PRM) was applied to gas chromatographic (GC) data for a fatty acid methyl esters (FAME) composition of commercial and laboratory synthesized biodiesel fuels from vegetable oils including sunflower, rapeseed, corn and palm oils. Two GC quantitative methods to calculate individual fames were compared: Area % and internal standard. The both methods were applied for analysis of two certified reference materials. The statistical processing of the obtained results demonstrates the accuracy and precision of the two methods and allows them to be compared. For further chemometric investigations of biodiesel fuels by their FAME-profiles any of those methods can be used. PRM results of FAME profiles of samples from different vegetable oils show a successful recognition of biodiesels according to the feedstock. The information obtained can be used for selection of feedstock to produce biodiesels with certain properties, for assessing their interchangeability, for fuel spillage and remedial actions in the environment.
Exopolysaccharides from lactic acid bacteria as corrosion inhibitors
Authors: Ignatova-Ivanova Tsveteslava , Ivanov Radoslav 
Published: March 2016

Abstract
Bacterial EPSs (exopolysaccharides) are believed to play an important role in the environment by promoting survival strategies such as bacterial attachment to surfaces and nutrient trapping, which facilitate processes of biofilm formation and development. These microbial biofilms have been implicated in corrosion of metals, bacterial attachment to prosthetic devices, fouling of heat exchange surfaces, toxicant immobilization, and fouling of ship hulls. In this paper, data on EPS production and the effect of EPS on corrosion of steel produced by Lactobacillus sp. are presented and discussed. Lactobacillus delbrueckii K27, Lactobacillus delbrueckii B8, Lactobacillus delbrueckii KO43, Lactobacillus delbrueckii K3, Lactobacillus delbrueckii K15 and Lactobacillus delbrueckii K17 was obtained from Collection of Department of General and Applied Microbiology, Sofia University. It was tested for its ability to produce exopolysaccharides when cultivated in a media containing 10% sucrose, 10% lacose and 10% maltose. The study of the corrosive stability of steel samples was conducted on the gravimetrique method. The rate of corrosion, the degree of protection, and coefficient of protection have been calculated. The structure of layer over steel plates was analysed by SEM (scanning electron microscopy) JSM 5510. It could be underlined that 10% sucrose, 10% lactose and 10% maltose in the media stimulated the process of protection of corrosion.
Cultural heritage of Varna and its tourist recognisability in Europe
Authors: Dombrowicz M. , Gruszka P. , Jarecka I. 
Published: March 2016

Abstract
Cultural heritage of the European civilization constitutes cultural and spiritual property of the ancestors, as well as current generations of the Varna city. It represents both material and non-material value, defines the European culture. It includes all the environmental consequences arising from the interaction between the man and the surroundings over the course of history. Assessment of tourist potential of Varna performed on 9-14th September, 2014, by the members of the “European Traveler” scientific circle, students of tourist and recreation, as well as heritage and culture tourism at Kazimierz Wielki University in Bydgoszcz, made it possible to identify the main points of tourist recognizability in relation to the city and region. The staid points are predominantly based on the unique cultural assets of the city and the region - in large measure related to the origins of the European civilization - as well as current cultural events organized in the city.
On axisymmetrical boundary problem of unsteady motion of micropolar fluid in the half-space
Authors: El-Sirafy I.H. , Aboutahoun A.W. 
Published: March 2016

Abstract
The aim of this paper is developing an exact solution for the problem of axisymmetrical flow of unsteady motion of micropolar fluid in the half-space when the shear stresses are given on the boundary. The Laplace-Hankel transform technique is used to solve this problem. Some physical quantities such as velocities, pressure and microrotations are obtained and illustrated numerically.
Implementation of factitious force method for control of 5R manipulator with skid-steering platform REX
Authors: Mazur A. , Cholewiński M. 
Published: March 2016

Abstract
In the paper implementation of the factitious force concept for a controlling complex mobile manipulator has been presented. As the nonholonomic constraint only lack of longitudinal slippage of wheels has been chosen - in skid-steering platforms lateral slippage is necessary to change orientation of such a platform. From a control theory point of view such a system is dynamically underactuated. As a solution to a underactuation problem a method of factitious force has been proposed. This method assumes extension on the dynamics level, in the form of an additional control inputs uv, which values are equal to zero equivalently. For a mobile manipulator, consisting of platform REX and 5R robotic onboard arm, a cascaded control law has been proposed. A simulation study was conducted for a mathematical model of a considered object with real values of physical parameters, i.e. lengths, masses, inertia moments etc. obtained from the 3D model. Results obtained in simulations have shown a proper action of the control system and convergence of tracking errors, occurring in a platform and in joints of a manipulator, to zero.
Streamer propagation in a non-uniform electric field under lightning impulse in short gaps insulated with natural ester and mineral oil
Authors: Rozga P. 
Published: March 2016

Abstract
This article describes the comparative experimental studies on streamer propagation in natural ester and mineral oil under a high voltage lightning impulse. These studies were concentrated around the small electrode gaps and the point-plane electrode arrangement. The spatial shapes of the developing streamers, light emission and propagation velocity were analyzed and compared between the two different dielectric liquids. In both of them two streamer propagation modes were registered during the studies performed. Propagation of slow 2nd mode streamers took place below the so-called acceleration voltage while fast 3rd mode streamers developed at acceleration voltage and above. Comparing the streamer shapes corresponding to a given voltage polarity, no visible differences were observed between the liquids tested. Concerning the light emission, higher frequencies of light pulses were registered however in the case of natural ester. The significant differences between both liquids were noticed in the value of the acceleration voltage estimated. In the case of positive polarity streamers started to develop in natural ester as a 3rd mode at lower value of testing voltage than in the case of mineral oil. For negative polarity, within the applied testing voltages, 3rd mode streamers appeared only in natural ester. On this basis, the fundamental conclusion is that natural ester may have a lower ability of preventing the development of fast and energetic 3rd mode streamers, even at small electrode gaps.
Controlling continuous bioreactor via nonlinear feedback: modelling and simulations approach
Authors: Aguilar-López R. , Neria-González I. 
Published: March 2016

Abstract
The aim of this work is to present a class of nonlinear controller with an exponential-type feedback in order to regulate the sulfate mass concentration via the input flow in a continuous stirred tank bioreactor of an anaerobic sulfate-reducing process. The corresponding kinetic terms in the bioreactor’s modeling are modeled by unstructured modeling approach, which was experimentally corroborated. A sketch of proof of the closed-loop stability of the considered system is done under the framework of Lyapunov theory. Numerical experiments are conducted to show the performance of the proposed methodology in comparison with a well-tuned sigmoid controller.
The application of hypergeometric functions to computing fractional order derivatives of sinusoidal functions
Authors: Włodarczyk M. , Zawadzki A. 
Published: March 2016

Abstract
In the paper, the analytical forms of fractional order derivatives of sinusoidal function according to definitions of Riemann - Liouville and Caputo are presented. To determine the analytical form of the integrals appearing in definitions of derivatives of fractional order the Lommel functions from hypergeometrical functions family were applied. With the use of properties of the derivatives of fractional order - differential-integral there were presented the conception of generalized element of a single equation, which depending on the value of the derivative order, can be inductor, resistor, capacitor, or a hypothetical element of a fractional order differential equation.
Failure modes of coatings on steel substrate
Authors: Mróz K.P. , Kucharski S. , Doliński K. , Bigos A. , Mikułowski G. , Beltowska-Lehman E. , Nolbrzak P. 
Published: March 2016

Abstract
The critical monotonic strain of Ni-W and MoS2(Ti,W) coatings on steel substrates was studied. The idea of axisymmetric bending test (called here as coin bending test) limited to monitoring of the coating failure was used. Experiments revealed mechanism of the coating failure, as cracking initiated from coating surface defects and/or substrate was demonstrated using indentation technique. By pushing the center of the uncoated side of a circular plate, the axisymmetric stress state was generated in the coating. The stress components varied gradually from the greatest value in the center to the smallest value at the edge of the specimen. The changes of the sample surface as a result of loading were monitored step by step via optical microscopy.