bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between recall of recently versus remotely encoded famous faces and amyloidosis in clinically normal older adults
Authors: Orlovsky Irina , Huijbers Willem , Hanseeuw Bernard J. , Mormino Elizabeth C. , Hedden Trey , Buckley Rachel F. , LaPoint Molly , Rabin Jennifer S. , Rentz Dorene M. , Johnson Keith A. , Sperling Reisa A. , Papp Kathryn V. 
Published: November 2017

AbstractIntroductionAlzheimer's disease (AD) patients exhibit temporally graded memory loss with remote memories remaining more intact than recent memories. It is unclear whether this temporal pattern is observable in clinically normal adults with amyloid pathology (i.e. preclinical AD).MethodsParticipants were asked to recall the names of famous figures most prominent recently (famous after 1990) and remotely (famous from 1960–1980) and were provided with a phonemic cue to ensure that memory failure was not purely due to verbal retrieval weaknesses. In addition, participants identified line drawings of objects. Clinically normal older adults (n = 125) were identified as amyloid β positive or negative (Aβ+/−) using Pittsburgh compound B positron emission tomography. The relationship between Aβ+/− and recall of remote and recent famous face‐names and objects was examined using repeated measures analyses and general linear models controlling for demographics and media usage.ResultsWhen provided with a phonemic cue, Aβ+ participants recalled the names of fewer recent famous faces compared with Aβ− participants. However, recall of remote famous face‐names and objects did not differ by Aβ group.DiscussionRelative sparing of remotely learned information compared with recently learned information is (1) detectable in the preclinical stages of AD and (2) related to amyloid pathology. Both this temporal gradient and assessment of person‐centered rather than object‐centered semantic information may be particularly meaningful for tracking early memory changes in the AD trajectory.
Development of an Intervention Programme for Selective Eating in Children with Autism Spectrum Disorder
Authors: Miyajima Ayumi , Tateyama Kiyomi , Fuji Shiori , Nakaoka Kazuyo , Hirao Kazuhisa , Higaki Kazuo 
Published: November 2017

Objective/Background Most parents of children with autism spectrum disorder (ASD) have difficulties with the selective eating behaviour of their children. This study aimed to develop a newly designed intervention programme on improving selective eating behaviour for parents of children with ASD and evaluate its effectiveness. Methods The participants were 23 parents of children (aged 3–6 years) with ASD. The education programme included a session that addressed approaches to improve selective eating and attitudes at meal times, with a discussion. The intervention aimed to identify the underlying factors and approaches to improve selective eating in children and the self-efficacy of parents. Results Significant differences were observed before and after the intervention in the degree of difficulty perceived by parents, their degree of self-efficacy, the number of recommendations conducted by them, their subjective view of the degree of dietary imbalance, and the number of food items consumed by their children. Conclusion We developed an interventional programme for parents of children with ASD and this programme was found to be useful. It is important for occupational therapists to consider the factors and approaches for selective eating in children with ASD in order to provide early intervention for their parents.
Aboutness Topics in Old and Middle French: A corpus-based study on the fate of V2
Authors: de Andrade Aroldo 
Published: November 2017

AbstractThis article investigates the loss of aboutness topics in preverbal position in the history of French, using a corpus-based research on preverbal accusative objects. A comparison of Old and Middle French with Modern French reveals that new-information focalization had disappeared by the 14thcentury, whereas aboutness topicalization had in turn vanished by the end of the 16thcentury, along with other marked constructions. Combined with the generative premise that independent pragmatic factors should not trigger syntactic change, the results of this study suggest the reanalysis of the grammar as V-to-I in Renaissance French is responsible for blocking the derivation of aboutness topicalization. An alternative proposal based on phase extension and on Relativized Minimality, in a version affecting some types of A′-movement, relates those two diachronic shifts. The article concludes with the idea that the study of marked constructions may be recast as offering diagnostics on broader syntactic changes.
Accommodation or political identity: Scottish members of the UK Parliament
Authors: Hall-Lew Lauren , Friskney Ruth , Scobbie James M. 
Published: November 2017

AbstractPhonetic variation among Scottish members of the UK Parliament may be influenced by convergence to Southern English norms (Carr & Brulard, 2006) or political identity (e.g., Hall-Lew, Coppock, & Starr, 2010). Drawing on a year's worth of political speeches (2011–2012) from 10 Scottish members of the UK Parliament (MPs), we find no acoustic evidence for the adoption of a Southern English low vowel system; rather, we find that vowel height is significantly correlated with political party: Scottish Labour Party MPs produce a highercatvowel (Johnston, 1997) than do Scottish National Party MPs. The results contradict claims that Scottish MPs acquire Anglo-English features while serving in the UK Parliament. Rather, we suggest that the variation indexes political meaning, with a subset of individuals drawing on that indexicality in production.
Scalar effects of social networks on language variation
Authors: Sharma Devyani 
Published: November 2017

AbstractThe role of social networks in language variation has been studied using a wide range of metrics. This study critically examines the effect of different dimensions of networks on different aspects of language variation. Three dimensions of personal network (ethnicity, nationality, diversity) are evaluated in relation to three levels of language structure (phonetic form, accent range, language choice) over three generations of British Asians. The results indicate a scaling of network influences. The two metrics relating to qualities of an individual's ties are more historically and culturally specific, whereas the network metric that relates to the structure of an individual's social world appears to exert a more general effect on accent repertoires across generations. This two-tier typology—network qualities (more culturally contingent) and network structures (more general)—facilitates an integrated understanding of previous studies and a more structured methodology for studying the effect of social networks on language.
Graphene-edge dielectrophoretic tweezers for trapping of biomolecules
Authors: Barik Avijit , Zhang Yao , Grassi Roberto , Nadappuram Binoy Paulose , Edel Joshua B. , Low Tony , Koester Steven J. , Oh Sang-Hyun 
Published: November 2017

AbstractThe many unique properties of graphene, such as the tunable optical, electrical, and plasmonic response make it ideally suited for applications such as biosensing. As with other surface-based biosensors, however, the performance is limited by the diffusive transport of target molecules to the surface. Here we show that atomically sharp edges of monolayer graphene can generate singular electrical field gradients for trapping biomolecules via dielectrophoresis. Graphene-edge dielectrophoresis pushes the physical limit of gradient-force-based trapping by creating atomically sharp tweezers. We have fabricated locally backgated devices with an 8-nm-thick HfO2dielectric layer and chemical-vapor-deposited graphene to generate 10× higher gradient forces as compared to metal electrodes. We further demonstrate near-100% position-controlled particle trapping at voltages as low as 0.45 V with nanodiamonds, nanobeads, and DNA from bulk solution within seconds. This trapping scheme can be seamlessly integrated with sensors utilizing graphene as well as other two-dimensional materials.
O-GlcNAcylation is required for B cell homeostasis and antibody responses
Authors: Wu Jung-Lin , Chiang Ming-Feng , Hsu Pan-Hung , Tsai Dong-Yen , Hung Kuo-Hsuan , Wang Ying-Hsiu , Angata Takashi , Lin Kuo-I 
Published: November 2017

AbstractO-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) catalyzes O-GlcNAc modification. O-GlcNAcylation is increased after cross-linking of the B-cell receptor (BCR), but the physiological function of this reaction is unknown. Here we show that lack of Ogt in B-cell development not only causes severe defects in the activation of BCR signaling, but also perturbs B-cell homeostasis by enhancing apoptosis of mature B cells, partly as a result of impaired response to B-cell activating factor. O-GlcNAcylation of Lyn at serine 19 is crucial for efficient Lyn activation and Syk interaction in BCR-mediated B-cell activation and expansion. Ogt deficiency in germinal center (GC) B cells also results in enhanced apoptosis of GC B cells and memory B cells in an immune response, consequently causing a reduction of antibody levels. Together, these results demonstrate that B cells rely on O-GlcNAcylation to maintain homeostasis, transduce BCR-mediated activation signals and activate humoral immunity.
Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1
Authors: Fujita Kyota , Mao Ying , Uchida Shigenori , Chen Xigui , Shiwaku Hiroki , Tamura Takuya , Ito Hikaru , Watase Kei , Homma Hidenori , Tagawa Kazuhiko , Sudol Marius , Okazawa Hitoshi 
Published: November 2017

AbstractYAP and its neuronal isoform YAPdeltaC are implicated in various cellular functions. We found that expression of YAPdeltaC during development, but not adulthood, rescued neurodegeneration phenotypes of mutant ataxin-1 knock-in (Atxn1-KI) mice. YAP/YAPdeltaC interacted with RORα via the second WW domain and served as co-activators of its transcriptional activity. YAP/YAPdeltaC formed a transcriptional complex with RORα on cis-elements of target genes and regulated their expression. Both normal and mutant Atxn1 interacted with YAP/YAPdeltaC, but only mutant Atxn1 depleted YAP/YAPdeltaC from the RORα complex to suppress transcription on short timescales. Over longer periods, mutant Atxn1 also decreased RORα in vivo. Genetic supplementation of YAPdeltaC restored the RORα and YAP/YAPdeltaC levels, recovered YAP/YAPdeltaC in the RORα complex and normalized target gene transcription in Atxn1-KI mice in vivo. Collectively, our data suggest that functional impairment of YAP/YAPdeltaC by mutant Atxn1 during development determines the adult pathology of SCA1 by suppressing RORα-mediated transcription.
In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure
Authors: Sittadjody Sivanandane , Saul Justin M. , McQuilling John P. , Joo Sunyoung , Register Thomas C. , Yoo James J. , Atala Anthony , Opara Emmanuel C. 
Published: November 2017

AbstractSafe clinical hormone replacement (HR) will likely become increasingly important in the growing populations of aged women and cancer patients undergoing treatments that ablate the ovaries. Cell-based HRT (cHRT) is an alternative approach that may allow certain physiological outcomes to be achieved with lower circulating hormone levels than pharmacological means due to participation of cells in the hypothalamus-pituitary-ovary feedback control loop. Here we describe the in vivo performance of 3D bioengineered ovarian constructs that recapitulate native cell–cell interactions between ovarian granulosa and theca cells as an approach to cHRT. The constructs are fabricated using either Ca++ or Sr++ to crosslink alginate. Following implantation in ovariectomized (ovx) rats, the Sr++-cross-linked constructs achieve stable secretion of hormones during 90 days of study. Further, we show these constructs with isogeneic cells to be effective in ameliorating adverse effects of hormone deficiency, including bone health, uterine health, and body composition in this rat model.
Inference of differentiation time for single cell transcriptomes using cell population reference data
Authors: Sun Na , Yu Xiaoming , Li Fang , Liu Denghui , Suo Shengbao , Chen Weiyang , Chen Shirui , Song Lu , Green Christopher D. , McDermott Joseph , Shen Qin , Jing Naihe , Han Jing-Dong J. 
Published: November 2017

AbstractSingle-cell RNA sequencing (scRNA-seq) is a powerful method for dissecting intercellular heterogeneity during development. Conventional trajectory analysis provides only a pseudotime of development, and often discards cell-cycle events as confounding factors. Here using matched cell population RNA-seq (cpRNA-seq) as a reference, we developed an “iCpSc” package for integrative analysis of cpRNA-seq and scRNA-seq data. By generating a computational model for reference “biological differentiation time” using cell population data and applying it to single-cell data, we unbiasedly associated cell-cycle checkpoints to the internal molecular timer of single cells. Through inferring a network flow from cpRNA-seq to scRNA-seq data, we predicted a role of M phase in controlling the speed of neural differentiation of mouse embryonic stem cells, and validated it through gene knockout (KO) experiments. By linking temporally matched cpRNA-seq and scRNA-seq data, our approach provides an effective and unbiased approach for identifying developmental trajectory and timing-related regulatory events.
Discovery of naturally occurring ESR1 mutations in breast cancer cell lines modelling endocrine resistance
Authors: Martin Lesley-Ann , Ribas Ricardo , Simigdala Nikiana , Schuster Eugene , Pancholi Sunil , Tenev Tencho , Gellert Pascal , Buluwela Laki , Harrod Alison , Thornhill Allan , Nikitorowicz-Buniak Joanna , Bhamra Amandeep , Turgeon Marc-Olivier , Poulogiannis George , Gao Qiong , Martins Vera , Hills Margaret , Garcia-Murillas Isaac , Fribbens Charlotte , Patani Neill , Li Zheqi , Sikora Matthew J. , Turner Nicholas , Zwart Wilbert , Oesterreich Steffi , Carroll Jason , Ali Simak , Dowsett Mitch 
Published: November 2017

AbstractResistance to endocrine therapy remains a major clinical problem in breast cancer. Genetic studies highlight the potential role of estrogen receptor-α (ESR1) mutations, which show increased prevalence in the metastatic, endocrine-resistant setting. No naturally occurring ESR1 mutations have been reported in in vitro models of BC either before or after the acquisition of endocrine resistance making functional consequences difficult to study. We report the first discovery of naturally occurring ESR1
Y537C
and ESR1
Y537S
mutations in MCF7 and SUM44 ESR1-positive cell lines after acquisition of resistance to long-term-estrogen-deprivation (LTED) and subsequent resistance to fulvestrant (ICIR). Mutations were enriched with time, impacted on ESR1 binding to the genome and altered the ESR1 interactome. The results highlight the importance and functional consequence of these mutations and provide an important resource for studying endocrine resistance.
Hepatic Crtc2 controls whole body energy metabolism via a miR-34a-Fgf21 axis
Authors: Han Hye-Sook , Choi Byeong Hun , Kim Jun Seok , Kang Geon , Koo Seung-Hoi 
Published: November 2017

AbstractLiver plays a crucial role in controlling energy homeostasis in mammals, although the exact mechanism by which it influences other peripheral tissues has yet to be addressed. Here we show that Creb regulates transcriptional co-activator (Crtc) 2 is a major regulator of whole-body energy metabolism. Crtc2 liver-specific knockout lowers blood glucose levels with improved glucose and insulin tolerance. Liver-specific knockout mice display increased energy expenditure with smaller lipid droplets in adipose depots. Both plasma and hepatic Fgf21 levels are increased in Crtc2 liver-specific knockout mice, as a result of the reduced miR-34a expression regulated by Creb/Crtc2 and the induction of Sirt1 and Pparα. Ectopic expression of miR-34a reverses the metabolic changes in knockout liver. We suggest that Creb/Crtc2 negatively regulates the Sirt1/Pparα/Fgf21 axis via the induction of miR-34a under diet-induced obesity and insulin-resistant conditions.
Inhibiting Rho kinase promotes goal-directed decision making and blocks habitual responding for cocaine
Authors: Swanson Andrew M. , DePoy Lauren M. , Gourley Shannon L. 
Published: November 2017

AbstractThe prelimbic prefrontal cortex is necessary for associating actions with their consequences, enabling goal-directed decision making. We find that the strength of action–outcome conditioning correlates with dendritic spine density in prelimbic cortex, suggesting that new action–outcome learning involves dendritic spine plasticity. To test this, we inhibited the cytoskeletal regulatory factor Rho kinase. We find that the inhibitor fasudil enhances action–outcome memory, resulting in goal-directed behavior in mice that would otherwise express stimulus-response habits. Fasudil transiently reduces prelimbic cortical dendritic spine densities during a period of presumed memory consolidation, but only when paired with new learning. Fasudil also blocks habitual responding for cocaine, an effect that persists over time, across multiple contexts, and depends on actin polymerization. We suggest that Rho kinase inhibition promotes goal-oriented action selection by augmenting the plasticity of prelimbic cortical dendritic spines during the formation of new action–outcome memories.
BEX1 is an RNA-dependent mediator of cardiomyopathy
Authors: Accornero Federica , Schips Tobias G. , Petrosino Jennifer M. , Gu Shan-Qing , Kanisicak Onur , van Berlo Jop H. , Molkentin Jeffery D. 
Published: November 2017

AbstractRegulation of mRNA splicing, processing and stability is increasingly recognized as a critical control point in dynamically altering gene expression during stress or disease. Very little is understood of this process in heart failure. Here, we show that BEX1 is a heart failure-induced gene functioning as an mRNA-associated protein that enhances expression of a subset of cardiac disease-promoting genes. Modeling the increase in BEX1 that occurs in disease, cardiac-specific BEX1 transgenic mice show worse cardiac disease with stress stimulation, whereas Bex1 gene-deleted mice are protected from heart failure-promoting insults. Proteomic and interactive screening assays show that BEX1 is part of a large ribonucleoprotein processing complex involved in regulating proinflammatory mRNA expression in the heart. Specifically, induction of BEX1 augments the stability and expression of AU-rich element containing mRNAs typically found within proinflammatory genes. Thus, BEX1 functions as an mRNA-dependent effector that augments pathology-promoting gene expression during heart failure.
Contributions of Zea mays subspecies mexicana haplotypes to modern maize
Authors: Yang Ning , Xu Xi-Wen , Wang Rui-Ru , Peng Wen-Lei , Cai Lichun , Song Jia-Ming , Li Wenqiang , Luo Xin , Niu Luyao , Wang Yuebin , Jin Min , Chen Lu , Luo Jingyun , Deng Min , Wang Long , Pan Qingchun , Liu Feng , Jackson David , Yang Xiaohong , Chen Ling-Ling , Yan Jianbing 
Published: November 2017

AbstractMaize was domesticated from lowland teosinte (Zea mays ssp. parviglumis), but the contribution of highland teosinte (Zea mays ssp. mexicana, hereafter mexicana) to modern maize is not clear. Here, two genomes for Mo17 (a modern maize inbred) and mexicana are assembled using a meta-assembly strategy after sequencing of 10 lines derived from a maize-teosinte cross. Comparative analyses reveal a high level of diversity between Mo17, B73, and mexicana, including three Mb-size structural rearrangements. The maize spontaneous mutation rate is estimated to be 2.17 × 10−8 ~3.87 × 10−8 per site per generation with a nonrandom distribution across the genome. A higher deleterious mutation rate is observed in the pericentromeric regions, and might be caused by differences in recombination frequency. Over 10% of the maize genome shows evidence of introgression from the mexicana genome, suggesting that mexicana contributed to maize adaptation and improvement. Our data offer a rich resource for constructing the pan-genome of Zea mays and genetic improvement of modern maize varieties.
The gibberellin GID1-DELLA signalling module exists in evolutionarily ancient conifers
Authors: Du Ran , Niu Shihui , Liu Yang , Sun Xinrui , Porth Ilga , El-Kassaby Yousry A. , Li Wei 
Published: November 2017

AbstractGibberellins (GAs) participate in controlling various aspects of basic plant growth responses. With the exception of bryophytes, GA signalling in land plants, such as lycophytes, ferns and angiosperms, is mediated via GIBBERELLIN-INSENSITIVE DWARF1 (GID1) and DELLA proteins. To explore whether this GID1-DELLA mechanism is present in pines, we cloned an orthologue (PtGID1) of Arabidopsis AtGID1a and two putative DELLA proteins (PtDPL; PtRGA) from Pinus tabuliformis, a widespread indigenous conifer species in China, and studied their recombinant proteins. PtGID1 shares with AtGID1a the conserved HSL motifs for GA binding and an N-terminal feature that are essential for interaction with DELLA proteins. Indeed, A. thaliana 35S:PtGID1 overexpressors showed a strong GA-hypersensitive phenotype compared to the wild type. Interactions between PtGID1 and PtDELLAs, but also interactions between the conifer-angiosperm counterparts (i.e. between AtGID1 and PtDELLAs and between PtGID1 and AtDELLA), were detected in vivo. This demonstrates that pine has functional GID1-DELLA components. The Δ17-domains within PtDPL and PtRGA were identified as potential interaction sites within PtDELLAs. Our results show that PtGID1 has the ability to interact with DELLA and functions as a GA receptor. Thus, a GA-GID1-DELLA signalling module also operates in evolutionarily ancient conifers.
Mechanism in External Field-mediated Trapping of Bacteria Sensitive to Nanoscale Surface Chemical Structure
Authors: Tokonami Shiho , Shimizu Emi , Tamura Mamoru , Iida Takuya 
Published: November 2017

AbstractMolecular imprinting technique enables the selective binding of nanoscale target molecules to a polymer film, within which their chemical structure is transcribed. Here, we report the successful production of mixed bacterial imprinted film (BIF) from several food poisoning bacteria by the simultaneous imprinting of their nanoscale surface chemical structures (SCS), and provide highly selective trapping of original micron-scale bacteria used in the production process of mixed BIF even for multiple kinds of bacteria in real samples. Particularly, we reveal the rapid specific identification of E. coli group serotypes (O157:H7 and O26:H11) using an alternating electric field and a quartz crystal microbalance. Furthermore, we have performed the detailed physicochemical analysis of the specific binding of SCS and molecular recognition sites (MRS) based on the dynamic Monte Carlo method under taking into account the electromagnetic interaction. The dielectrophoretic selective trapping greatly depends on change in SCS of bacteria damaged by thermal treatment, ultraviolet irradiation, or antibiotic drugs, which can be well explained by the simulation results. Our results open the avenue for an innovative means of specific and rapid detection of unknown bacteria for food safety and medicine from a nanoscale viewpoint.