bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single coronary artery with a pre-pulmonic dual left anterior descending artery and a retro-aortic left circumflex artery
Authors: Shah Soham , Rajiah Prabhakar 
Published: May 2016

AbstractWe present two cases of an unusual coronary artery anomaly, namely, a single coronary artery with retro-aortic course of the left circumflex artery and pre-pulmonic course of the left anterior descending artery, and one of which showed a dual left anterior descending artery as well – a combination that has not yet been reported in the literature.
Sprachkompetenz als Prädiktor mathematischer Kompetenzentwicklung von Kindern deutscher und nicht-deutscher Familiensprache
Authors: Paetsch Jennifer , Radmann Susanne , Felbrich Anja , Lehmann Rainer , Stanat Petra 
Published: May 2016

Zusammenfassung. Das Ziel des vorliegenden Beitrages bestand darin, anhand der längsschnittlich angelegten Studie Erhebungen zum Lese- und Mathematikverständnis–Entwicklungen in den Jahrgangsstufen 4 bis 6 in Berlin (ELEMENT) den Einfluss von Lesekompetenz auf die Kompetenzentwicklung in Mathematik bei Schülerinnen und Schülern von der 4. bis zur 6. Jahrgangsstufe (N = 3 169) zu analysieren. Darüber hinaus wurde untersucht, ob Kinder nicht-deutscher Familiensprache unter Kontrolle des sozioökonomischen Status (SES) und der allgemeinen kognitiven Grundfähigkeiten geringere Lernzuwächse in Mathematik erzielen als Kinder deutscher Familiensprache. Der Entwicklungsverlauf der Mathematikkompetenz wurde dabei anhand eines Wachstumskurvenmodells analysiert. Erwartungskonform zeigte sich, dass die Lesekompetenz, auch unter Kontrolle des SES und der allgemeinen kognitiven Grundfähigkeiten, nicht nur signifikant mit der mathematischen Ausgangskompetenz zusammenhing, sondern darüber hinaus auch einen signifikanten Beitrag zur Vorhersage der mathematischen Lernzuwachsraten aller Schülerinnen und Schüler leistete. Obwohl die bestehenden Kompetenzunterschiede in Mathematik zwischen Kindern nicht-deutscher Familiensprache und deutscher Familiensprache größtenteils auf die Lesekompetenz zurückführbar waren, vergrößerten sich die Disparitäten zwischen Kindern deutscher und Kindern nicht-deutscher Familiensprache nicht.
'Mitochondrial energy imbalance and lipid peroxidation cause cell death in Friedreich’s ataxia'
Authors: Abeti R , Parkinson M H , Hargreaves I P , Angelova P R , Sandi C , Pook M A , Giunti P , Abramov A Y 
Published: May 2016

AbstractFriedreich’s ataxia (FRDA) is an inherited neurodegenerative disease. The mutation consists of a GAA repeat expansion within the FXN gene, which downregulates frataxin, leading to abnormal mitochondrial iron accumulation, which may in turn cause changes in mitochondrial function. Although, many studies of FRDA patients and mouse models have been conducted in the past two decades, the role of frataxin in mitochondrial pathophysiology remains elusive. Are the mitochondrial abnormalities only a side effect of the increased accumulation of reactive iron, generating oxidative stress? Or does the progressive lack of iron-sulphur clusters (ISCs), induced by reduced frataxin, cause an inhibition of the electron transport chain complexes (CI, II and III) leading to reactive oxygen species escaping from oxidative phosphorylation reactions? To answer these crucial questions, we have characterised the mitochondrial pathophysiology of a group of disease-relevant and readily accessible neurons, cerebellar granule cells, from a validated FRDA mouse model. By using live cell imaging and biochemical techniques we were able to demonstrate that mitochondria are deregulated in neurons from the YG8R FRDA mouse model, causing a decrease in mitochondrial membrane potential (▵Ψm) due to an inhibition of Complex I, which is partially compensated by an overactivation of Complex II. This complex activity imbalance leads to ROS generation in both mitochondrial matrix and cytosol, which results in glutathione depletion and increased lipid peroxidation. Preventing this increase in lipid peroxidation, in neurons, protects against in cell death. This work describes the pathophysiological properties of the mitochondria in neurons from a FRDA mouse model and shows that lipid peroxidation could be an important target for novel therapeutic strategies in FRDA, which still lacks a cure.
The emerging role of Toll-like receptor 4 in myocardial inflammation
Authors: Yang Y , Lv J , Jiang S , Ma Z , Wang D , Hu W , Deng C , Fan C , Di S , Sun Y , Yi W 
Published: May 2016

AbstractToll-like receptors (TLRs) are a family of pattern recognition receptors involved in cardiovascular diseases. Notably, numerous studies have demonstrated that TLR4 activates the expression of several of pro-inflammatory cytokine genes that play pivotal roles in myocardial inflammation, particularly myocarditis, myocardial infarction, ischemia-reperfusion injury, and heart failure. In addition, TLR4 is an emerging target for anti-inflammatory therapies. Given the significance of TLR4, it would be useful to summarize the current literature on the molecular mechanisms and roles of TLR4 in myocardial inflammation. Thus, in this review, we first introduce the basic knowledge of the TLR4 gene and describe the activation and signaling pathways of TLR4 in myocardial inflammation. Moreover, we highlight the recent progress of research on the involvement of TLR4 in myocardial inflammation. The information reviewed here may be useful to further experimental research and to increase the potential of TLR4 as a therapeutic target.
EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice
Authors: Thomas Geethu E. , Bandopadhyay K. , Sutradhar Sabyasachi , Renjith M. R. , Singh Puja , Gireesh K. K. , Simon Steny , Badarudeen Binshad , Gupta Hindol , Banerjee Manidipa , Paul Raja , Mitra J. , Manna Tapas K. 
Published: May 2016

AbstractKinetochore couples chromosome movement to dynamic microtubules, a process that is fundamental to mitosis in all eukaryotes but poorly understood. In vertebrates, spindle-kinetochore-associated (Ska1–3) protein complex plays an important role in this process. However, the proteins that stabilize Ska-mediated kinetochore-microtubule attachment remain unknown. Here we show that microtubule plus-end tracking protein EB1 facilitates Ska localization on microtubules in vertebrate cells. EB1 depletion results in a significant reduction of Ska1 recruitment onto microtubules and defects in mitotic chromosome alignment, which is also reflected in computational modelling. Biochemical experiments reveal that EB1 interacts with Ska1, facilitates Ska1-microtubule attachment and together stabilizes microtubules. Structural studies reveal that EB1 either with Ska1 or Ska complex forms extended structures on microtubule lattice. Results indicate that EB1 promotes Ska association with K-fibres and facilitates kinetochore-microtubule attachment. They also implicate that in vertebrates, chromosome coupling to dynamic microtubules could be mediated through EB1-Ska extended structures.
Electron–phonon coupling in hybrid lead halide perovskites
Authors: Wright Adam D. , Verdi Carla , Milot Rebecca L. , Eperon Giles E. , Pérez-Osorio Miguel A. , Snaith Henry J. , Giustino Feliciano , Johnston Michael B. , Herz Laura M. 
Published: May 2016

AbstractPhonon scattering limits charge-carrier mobilities and governs emission line broadening in hybrid metal halide perovskites. Establishing how charge carriers interact with phonons in these materials is therefore essential for the development of high-efficiency perovskite photovoltaics and low-cost lasers. Here we investigate the temperature dependence of emission line broadening in the four commonly studied formamidinium and methylammonium perovskites, HC(NH2)2PbI3, HC(NH2)2PbBr3, CH3NH3PbI3 and CH3NH3PbBr3, and discover that scattering from longitudinal optical phonons via the Fröhlich interaction is the dominant source of electron–phonon coupling near room temperature, with scattering off acoustic phonons negligible. We determine energies for the interacting longitudinal optical phonon modes to be 11.5 and 15.3 meV, and Fröhlich coupling constants of ∼40 and 60 meV for the lead iodide and bromide perovskites, respectively. Our findings correlate well with first-principles calculations based on many-body perturbation theory, which underlines the suitability of an electronic band-structure picture for describing charge carriers in hybrid perovskites.
Content and quality of websites supporting self-management of chronic breathlessness in advanced illness: a systematic review
Authors: Luckett Tim , Disler Rebecca , Hosie Annmarie , Johnson Miriam , Davidson Patricia , Currow David , Sumah Anthony , Phillips Jane 
Published: May 2016

AbstractChronic breathlessness is a common, burdensome and distressing symptom in many advanced chronic illnesses. Self-management strategies are essential to optimise treatment, daily functioning and emotional coping. People with chronic illness commonly search the internet for advice on self-management. A review was undertaken in June 2015 to describe the content and quality of online advice on breathlessness self-management, to highlight under-served areas and to identify any unsafe content. Google was searched from Sydney, Australia, using the five most common search terms for breathlessness identified by Google Trends. We also hand-searched the websites of national associations. Websites were included if they were freely available in English and provided practical advice on self-management. Website quality was assessed using the American Medical Association Benchmarks. Readability was assessed using the Flesch–Kincaid grades, with grade 8 considered the maximum acceptable for enabling access. Ninety-one web pages from 44 websites met the inclusion criteria, including 14 national association websites not returned by Google searches. Most websites were generated in the USA (n=28, 64%) and focused on breathing techniques (n=38, 86%) and chronic obstructive pulmonary disease (n=27, 61%). No websites were found to offer unsafe advice. Adherence to quality benchmarks ranged from 9% for disclosure to 77% for currency. Fifteen (54%) of 28 written websites required grade ⩾9 reading level. Future development should focus on advice and tools to support goal setting, problem solving and monitoring of breathlessness. National associations are encouraged to improve website visibility and comply with standards for quality and readability.
Assembling Bare Au Nanoparticles at Positively Charged Templates
Authors: Wang Wenjie , Zhang Honghu , Kuzmenko Ivan , Mallapragada Surya , Vaknin David 
Published: May 2016

AbstractIn-situ X-ray reflectivity (XRR) and grazing incidence X-ray small-angle scattering (GISAXS) reveal that unfunctionalized (bare) gold nanoparticles (AuNP) spontaneously adsorb to a cationic lipid template formed by a Langmuir monolayer of DPTAP (1,2-dihexadecanoyl-3-trimethylammonium-propane) at vapor/aqueous interfaces. Analysis of the XRR yields the electron density profile across the charged-interfaces along the surface normal showing the AuNPs assemble with vertical thickness comparable to the particle size. The GISAXS analysis indicates that the adsorbed mono-particle layer exhibits short-range in-plane correlations. By contrast, single-stranded DNA-functionalized AuNPs, while attracted to the positively charged surface (more efficiently with the addition of salt to the solution), display less in-plane regular packing compared to bare AuNPs.
Neuron-Like Networks Between Ribosomal Proteins Within the Ribosome
Authors: Poirot Olivier , Timsit Youri 
Published: May 2016

AbstractFrom brain to the World Wide Web, information-processing networks share common scale invariant properties. Here, we reveal the existence of neural-like networks at a molecular scale within the ribosome. We show that with their extensions, ribosomal proteins form complex assortative interaction networks through which they communicate through tiny interfaces. The analysis of the crystal structures of 50S eubacterial particles reveals that most of these interfaces involve key phylogenetically conserved residues. The systematic observation of interactions between basic and aromatic amino acids at the interfaces and along the extension provides new structural insights that may contribute to decipher the molecular mechanisms of signal transmission within or between the ribosomal proteins. Similar to neurons interacting through “molecular synapses”, ribosomal proteins form a network that suggest an analogy with a simple molecular brain in which the “sensory-proteins” innervate the functional ribosomal sites, while the “inter-proteins” interconnect them into circuits suitable to process the information flow that circulates during protein synthesis. It is likely that these circuits have evolved to coordinate both the complex macromolecular motions and the binding of the multiple factors during translation. This opens new perspectives on nanoscale information transfer and processing.
Construction of plasmid-free Escherichia coli for the production of arabitol-free xylitol from corncob hemicellulosic hydrolysate
Authors: Su Buli , Zhang Zhe , Wu Mianbin , Lin Jianping , Yang Lirong 
Published: May 2016

AbstractHigh costs and low production efficiency are a serious constraint to bio-based xylitol production. For industrial-scale production of xylitol, a plasmid-free Escherichia coli for arabitol-free xylitol production from corncob hemicellulosic hydrolysate has been constructed. Instead of being plasmid and inducer dependent, this strain relied on multiple-copy integration of xylose reductase (XR) genes into the chromosome, where their expression was controlled by the constitutive promoter P43. In addition, to minimize the flux from L-arabinose to arabitol, two strategies including low XR total activity and high selectivity of XR has been adopted. Arabitol was significantly decreased using plasmid-free strain which had lower XR total activity and an eight point-mutations of XR with a 27-fold lower enzyme activity toward L-arabinose was achieved. The plasmid-free strain in conjunction with this mutant XR can completely eliminate arabitol formation in xylitol production. In fed-batch fermentation, this plasmid-free strain produced 143.8 g L−1 xylitol at 1.84 g L−1 h−1 from corncob hemicellulosic hydrolysate. From these results, we conclude that this route by plasmid-free E. coli has potential to become a commercially viable process for xylitol production.
Structural characterization of CYP144A1 – a cytochrome P450 enzyme expressed from alternative transcripts in Mycobacterium tuberculosis
Authors: Chenge Jude , Kavanagh Madeline E. , Driscoll Max D. , McLean Kirsty J. , Young Douglas B. , Cortes Teresa , Matak-Vinkovic Dijana , Levy Colin W. , Rigby Stephen E. J. , Leys David , Abell Chris , Munro Andrew W. 
Published: May 2016

AbstractMycobacterium tuberculosis (Mtb) causes the disease tuberculosis (TB). The virulent Mtb H37Rv strain encodes 20 cytochrome P450 (CYP) enzymes, many of which are implicated in Mtb survival and pathogenicity in the human host. Bioinformatics analysis revealed that CYP144A1 is retained exclusively within the Mycobacterium genus, particularly in species causing human and animal disease. Transcriptomic annotation revealed two possible CYP144A1 start codons, leading to expression of (i) a “full-length” 434 amino acid version (CYP144A1-FLV) and (ii) a “truncated” 404 amino acid version (CYP144A1-TRV). Computational analysis predicted that the extended N-terminal region of CYP144A1-FLV is largely unstructured. CYP144A1 FLV and TRV forms were purified in heme-bound states. Mass spectrometry confirmed production of intact, His6-tagged forms of CYP144A1-FLV and -TRV, with EPR demonstrating cysteine thiolate coordination of heme iron in both cases. Hydrodynamic analysis indicated that both CYP144A1 forms are monomeric. CYP144A1-TRV was crystallized and the first structure of a CYP144 family P450 protein determined. CYP144A1-TRV has an open structure primed for substrate binding, with a large active site cavity. Our data provide the first evidence that Mtb produces two different forms of CYP144A1 from alternative transcripts, with CYP144A1-TRV generated from a leaderless transcript lacking a 5′-untranslated region and Shine-Dalgarno ribosome binding site.
Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel
Authors: Yang Jing , Guo Baocheng , Shikano Takahito , Liu Xiaolin , Merilä Juha 
Published: May 2016

AbstractHeritable phenotypic differences between populations, caused by the selective effects of distinct environmental conditions, are of commonplace occurrence in nature. However, the actual genomic targets of this kind of selection are still poorly understood. We conducted a quantitative trait locus (QTL) mapping study to identify genomic regions responsible for morphometric differentiation between genetically and phenotypically divergent marine and freshwater nine-spined stickleback (Pungitius pungitius) populations. Using a dense panel of SNP-markers obtained by restriction site associated DNA sequencing of an F2recombinant cross, we found 22 QTL that explained 3.5–12.9% of phenotypic variance in the traits under investigation. We detected one fairly large-effect (PVE = 9.6%) QTL for caudal peduncle length–a trait with a well-established adaptive function showing clear differentiation among marine and freshwater populations. We also identified two large-effect QTL for lateral plate numbers, which are different from the lateral plate QTL reported in earlier studies of this and related species. Hence, apart from identifying several large-effect QTL in shape traits showing adaptive differentiation in response to different environmental conditions, the results suggest intra- and interspecific heterogeneity in the genomic basis of lateral plate number variation.
Anomalous domain periodicity observed in ferroelectric PbTiO3 nanodots having 180° stripe domains
Authors: Son Jong Yeog , Song Seungwoo , Lee Jung-Hoon , Jang Hyun Myung 
Published: May 2016

AbstractNanometer-scale ferroelectric dots and tubes have received a great deal of attention owing to their potential applications to nonvolatile memories and multi-functional devices. As for the size effect of 180° stripe domains in ferroelectric thin films, there have been numerous reports on the thickness-dependent domain periodicity. All these studies have revealed that the domain periodicity (w) of 180° stripe domains scales with the film thickness (d) according to the classical Landau-Lifshitz-Kittel (LLK) scaling law (w ∝ d1/2) down to the thickness of ~2 nm. In the case of PbTiO3 nanodots, however, we obtained a striking correlation that for the thickness less than a certain critical value, dc (~35 nm), the domain width even increases with decreasing thickness of the nanodot, which surprisingly indicates a negative value in the LLK scaling-law exponent. On the basis of theoretical considerations of dc, we attributed this anomalous domain periodicity to the finite lateral-size effect of a ferroelectric nanodot with an additional effect possibly coming from the existence of a thin non-ferroelectric surface layer.
Large-Scale Production of Large-Size Atomically Thin Semiconducting Molybdenum Dichalcogenide Sheets in Water and Its Application for Supercapacitor
Authors: Chen Yu-Xiang , Wu Chien-Wei , Kuo Ting-Yang , Chang Yu-Lung , Jen Ming-Hsing , Chen I-Wen Peter 
Published: May 2016

AbstractTo progress from laboratory research to commercial applications, it is necessary to develop an effective method to prepare large quantities and high-quality of the large-size atomically thin molybdenum dichalcogenides (MoS2). Aqueous-phase processes provide a viable method for producing thin MoS2sheets using organolithium-assisted exfoliation; unfortunately, this method is hindered by changing pristine semiconducting 2H phase to distorted metallic 1T phase. Recovery of the intrinsic 2H phase typically involves heating of the 1T MoS2sheets on solid substrates at high temperature. This has restricted and hindered the utilization of 2H phase MoS2sheets suspensions. Here, we demonstrate that the synergistic effect of the rigid planar structure and charged nature of organic salt such as imidazole (ImH) can be successfully used to produce atomically thin 2H-MoS2sheets suspension in water. Moreover, lateral size and area of the exfoliated sheet can be up to 50 μm and 1000 μm2, respectively. According to the XPS measurements, nearly 100% of the 2H-MoS2sheets was successfully prepared. A composite paper supercapacitor using the exfoliated 2H-MoS2and carbon nanotubes delivered a superior volumetric capacitance of ~410 F/cm3. Therefore, the organic salts-assisted liquid-phase exfoliation has great potential for large-scale production of 2H-MoS2suspensions for supercapacitor application.
Experimental Demonstration of a Hybrid-Quantum-Emitter Producing Individual Entangled Photon Pairs in the Telecom Band
Authors: Chen Geng , Zou Yang , Zhang Wen-Hao , Zhang Zi-Huai , Zhou Zong-Quan , He De-Yong , Tang Jian-Shun , Liu Bi-Heng , Yu Ying , Zha Guo-Wei , Ni Hai-Qiao , Niu Zhi-Chuan , Han Yong-Jian , Li Chuan-Feng , Guo Guang-Can 
Published: May 2016

AbstractQuantum emitters generating individual entangled photon pairs (IEPP) have significant fundamental advantages over schemes that suffer from multiple photon emission, or schemes that require post-selection techniques or the use of photon-number discriminating detectors. Quantum dots embedded within nanowires (QD-NWs) represent one of the most promising candidate for quantum emitters that provide a high collection efficiency of photons. However, a quantum emitter that generates IEPP in the telecom band is still an issue demanding a prompt solution. Here, we demonstrate in principle that IEPPs in the telecom band can be created by combining a single QD-NW and a nonlinear crystal waveguide. The QD-NW system serves as the single photon source, and the emitted visible single photons are split into IEPPs at approximately 1.55 μm through the process of spontaneous parametric down conversion (SPDC) in a periodically poled lithium niobate (PPLN) waveguide. The compatibility of the QD-PPLN interface is the determinant factor in constructing this novel hybrid-quantum-emitter (HQE). Benefiting from the desirable optical properties of QD-NWs and the extremely high nonlinear conversion efficiency of PPLN waveguides, we successfully generate IEPPs in the telecom band with the polarization degree of freedom. The entanglement of the generated photon pairs is confirmed by the entanglement witness. Our experiment paves the way to producing HQEs inheriting the advantages of multiple systems.