bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic obesity phenotypes and the risk of cancer: a prospective study of the Kailuan cohort
Authors: Zheng Xin , Wang Yiming , Chen Yue , Liu Tong , Liu Chenan , Lin Shiqi , Xie Hailun , Ma Xiangming , Wang Ziwen , Shi Jinyu , Zhang Heyang , Yang Ming , Liu Xiaoyue , Deng Li , Zhang Qingsong , Shi Hanping 
Published: October 2024

BackgroundObesity is as an important risk factor for chronic diseases. Metabolically healthy obesity (MHO) is considered a benign state. The association between metabolic health and obesity categories and cancer risk remains unclear. This study aimed to investigate the relationship between metabolic health status combined with obesity phenotypes and the risk of cancer.MethodsData from 91,834 participants in the Kailuan cohort were analyzed, excluding individuals with a body mass index (BMI) &lt; 18.5 kg/m² and those with a history of cancer. Obesity phenotypes were classified based on BMI and waist circumference (WC) combined with metabolic health status, resulting in six phenotypes. Cox proportional hazard regression models were used to assess the association between metabolic health and obesity phenotypes with cancer risk and all-cause mortality.ResultsThe prevalence of metabolically healthy obesity and metabolically unhealthy obesity defined by BMI was 6.86% and 12.18%, while that defined by WC was 20.79% and 25.76%, respectively. Compared to metabolically healthy participants, individuals with an unhealthy metabolic status had a significantly higher risk of cancer (HR, 1.09; 95% CI, 1.03–1.15; p=0.004). The hazard ratios for cancer were 1.19, 1.23, 1.20, and 1.55 for individuals with one, two, three, and four metabolic disorders, respectively. Among those classified as metabolically unhealthy, both overweight and obesity were associated with a protective effect on cancer risk (HR, 0.88; 95% CI, 0.80–0.96; p=0.006 for overweight; HR, 0.87; 95% CI, 0.78–0.97; p=0.010 for obesity). However, abdominal obesity significantly increased cancer risk in both metabolically healthy and unhealthy participants. In subgroup analysis, simple obesity showed a protective trend against cancer in those with respiratory cancers, while abdominal obesity consistently posed a risk for various cancer types.ConclusionMetabolically unhealthy status and abdominal obesity are risk factors for cancer and all-cause mortality, whereas simple obesity offers protective effects against cancer and all-cause mortality in metabolically unhealthy individuals. These findings suggest that maintaining metabolic health and reducing the metabolic risks associated with abdominal obesity should be key targets for cancer prevention.
Objectively measured levels of moderate to vigorous intensity physical activity are associated with cognitive impairment in diabetic hemodialysis patients: a cross-sectional study
Authors: Zhang Zhixin , Chen Xiaoyu , Gaowa Siqin , Liang Ruiting , Jin Zhetong , Shi Ziyi , Han Peipei , Lin Cheng , Wen Xiaoli , Lin Peng , Guo Qi 
Published: October 2024

ObjectiveThe purpose of this study was to observe the relationship between objectively measured levels of physical activity and cognitive impairment (CI) in the presence or absence of diabetes in middle-aged and elderly hemodialysis patients.MethodsIn this multicenter cross-sectional study, 339 clinically stable hemodialysis patients (210 males; mean age: 67.38 ± 8.07 years) aged ≥55 years were included from 7 dialysis units in Shanghai, China. The Chinese version of the Modified Mini-Mental State Examination (MMSE) was used to assess the CI. The duration of physical activity at different intensities, including moderate to vigorous physical activity (MVPA) as well as light physical activity (LPA), was measured using a triaxial accelerometer (ActiGraph GT3X+, Pensacola, FL, USA). Logistic regression and multiple linear regression were used for analyses.ResultsThe prevalence of CI was higher in hemodialysis patients with comorbid diabetes (24.3%). In diabetic patients, MVPA (increase per 10 min/day) was negatively associated with CI after adjusting for covariates [(OR = 0.89, 95%CI = 0.79–0.99), p = 0.042]. However, no significant association between physical activity and CI was found in non-diabetic hemodialysis patients. Further analyses revealed that MVPA was positively associated with temporal orientation, attention and calculation and recall in diabetic hemodialysis patients.ConclusionPhysical activity was associated with CI in diabetic hemodialysis patients rather than the non-diabetes group. This study is important for early differential diagnosis of CI and improvement of cognitive status in hemodialysis patients.
Identification of key genes and diagnostic model associated with circadian rhythms and Parkinson’s disease by bioinformatics analysis
Authors: Zhang Jiyuan , Ma Xiaopeng , Li Zhiguang , Liu Hu , Tian Mei , Wen Ya , Wang Shan , Wang Liang 
Published: October 2024

BackgroundCircadian rhythm disruption is typical in Parkinson’s disease (PD) early stage, and it plays an important role in the prognosis of the treatment effect in the advanced stage of PD. There is growing evidence that circadian rhythm genes can influence development of PD. Therefore, this study explored specific regulatory mechanism of circadian genes (C-genes) in PD through bioinformatic approaches.MethodsDifferentially expressed genes (DEGs) between PD and control samples were identified from GSE22491 using differential expression analysis. The key model showing the highest correlation with PD was derived through WGCNA analysis. Then, DEGs, 1,288 C-genes and genes in key module were overlapped for yielding differentially expressed C-genes (DECGs), and they were analyzed for LASSO and SVM-RFE for yielding critical genes. Meanwhile, from GSE22491 and GSE100054, receiver operating characteristic (ROC) was implemented on critical genes to identify biomarkers, and Gene Set Enrichment Analysis (GSEA) was applied for the purpose of exploring pathways involved in biomarkers. Eventually, immune infiltrative analysis was applied for understanding effect of biomarkers on immune microenvironment, and therapeutic drugs which could affect biomarkers expressions were also predicted. Finally, we verified the expression of the genes by q-PCR.ResultsTotally 634 DEGs were yielded between PD and control samples, and MEgreen module had the highest correlation with PD, thus it was defined as key model. Four critical genes (AK3, RTN3, CYP4F2, and LEPR) were identified after performing LASSO and SVM-RFE on 18 DECGs. Through ROC analysis, AK3, RTN3, and LEPR were identified as biomarkers due to their excellent ability to distinguish PD from control samples. Besides, biomarkers were associated with Parkinson’s disease and other functional pathways.ConclusionThrough bioinformatic analysis, the circadian rhythm related biomarkers were identified (AK3, RTN3 and LEPR) in PD, contributing to studies related to PD treatment.
Cinobufotalin inhibits proliferation, migration and invasion in hepatocellular carcinoma by triggering NOX4/NLRP3/GSDMD-dependent pyroptosis
Authors: Liu Chen , Wu Jianmin , Li Zhiwen , Huang Xuanyu , Xie Xianhe , Huang Yun 
Published: October 2024

IntroductionPyroptosis is an inflammatory form of programmed cell death that plays a significant role in tumorigenesis. Cinobufotalin (CB), a bufadienolide extracted from toad venom, is associated with antitumor effects in various cancers, including liver cancer. However, the role of CB in pyroptosis and its underlying mechanisms have not been well characterized.MethodsMTT, Colony formation, EdU, Wound healing and Transwell migration and invasion assays were applied to determine the effects of CB on the proliferation, migration, and invasion ability of hepatocellular carcinoma (HCC) cells in vitro. The subcutaneous xenograft mouse model and pulmonary metastasis model were used to evaluate the effect of CB on HCC cells in vivo. PCR, western blot, immunohistochemistry, immunofluorescence, and ELISA were used to verify the expression of proliferation, migration, pyroptosis, and inflammation related molecules after CB treatment. Using si-RNA and inhibitors to interfere with NOX4 and HLRP3 expression to validate the key signaling pathways of pyroptosis induced by CB treatment.ResultsIn vivo experiments using nude mice with xenografted HCC cells and in vitro experiments with HCC cell lines demonstrated that CB treatment significantly inhibited the proliferation, migration, and invasiveness of HCC cells. CB treatment also showed dose-dependent activation of the NLRP3 inflammasome complex in HCC cells, leading to gasdermin D-induced pyroptosis. However, these effects were abrogated via the pretreatment of HCC cells with VX-765, a caspase-1 inhibitor. Additionally, CB increased the production of reactive oxygen species (ROS) and H₂O₂, along with upregulating NOX4 protein expression in HCC cells. Conversely, NOX4 silencing or pretreatment with VAS2870 (an NOX4 inhibitor) or NAC (an ROS scavenger) suppressed the activation of the NLRP3 inflammasome complex and pyroptosis in CB-treated HCC cells.DiscussionOur study demonstrated that CB suppressed the proliferation, migration, and invasiveness of HCC cells by inducing pyroptosis through the activation of the NOX4/NLRP3/GSDMD signaling pathway. Therefore, our results suggest that CB is a promising therapeutic agent for HCC.
Noise suppression of infrared thermal imaging of rocket exhaust plume using SPOD
Authors: Fu Debin , Sun Ao , Niu Qinglin 
Published: October 2024

The environmental noise have a negative influence on the quality of infrared thermal imaging of the rocket exhaust plume. In this study, the noise data of the unsteady rocket exhaust plume flow field was simulated using Gaussian white noise, and the infrared thermal image of the plume was numerically calculated using the narrow band method (SNB) and the line of sight (LOS) method. The denoising of infrared thermal imaging was achieved through the spectral proper orthogonal decomposition (SPOD) inversion method. Results indicate that Gaussian white noise leads to larger infrared thermal image residuals in the intrinsic core of the plume compared to the mixed regions. The infrared thermal image in the 2.7 μm band is greatly affected by the noise with an average error of 21.1%, and the average error in the 4.3 μm band is 17.6%. After SPOD denoising, the error of the plume infrared thermal image is reduced by more than 50%.
Parents’ rearing styles and adolescents’ math achievement: the multiple mediating effect of self-control and math anxiety
Authors: Wang Yanjiao , Han Lei , Tao Yang , Ma Yanyan 
Published: October 2024

IntroductionThis cross-sectional study examined the mechanisms underlying adolescent math achievement by investigating the relationship between parents’ rearing styles (including different dimensions of rearing style) and adolescent self-control, math anxiety, and math achievement based on the ecological systems theory.MethodA total of 584 junior high school students (M age = 12.52) completed the Parenting Style Questionnaire, Self-control Scale, and Math Anxiety Rating Scale and provided their math test scores.ResultsThe rearing styles of both fathers and mothers directly predicted adolescents’ math achievement. Maternal rearing style indirectly predicted adolescents’ math achievement through their self-control and math anxiety; however, the indirect effect of paternal rearing style on adolescents’ math achievement was not significant. After distinguishing the three dimensions of rearing styles, we found that paternal emotional warmth can increase adolescents’ self-control, while maternal emotional warmth can reduce adolescents’ self-control. Further, paternal overprotectiveness can directly and positively predict adolescents’ math achievement, while maternal rejection and overprotectiveness can positively predict adolescents’ math achievement. None of the three dimensions of rearing styles can predict math achievement through adolescents’ self-control; however, they can predict math achievement indirectly through adolescents’ math anxiety and the chain-mediation of adolescents’ self-control and math anxiety.DiscussionOur results suggest both commonalities and differences in how paternal and maternal rearing styles, along with their three dimensions (emotional warmth, rejection, overprotection), predict adolescent math achievement. These findings highlight the importance of paternal and maternal rearing styles on adolescents’ math achievement and underscore the need to examine them separately to better understand their impact.
Optimising availability and geographical accessibility to emergency obstetric care within a sub-national social health insurance scheme in Nigeria
Authors: Banke-Thomas Aduragbemi , Olubodun Tope , Olaniran Abimbola A. , Wong Kerry L. M. , Shah Yash , Achugo Daniel C. , Ogunyemi Olakunmi 
Published: October 2024

IntroductionHealth insurance is a key instrument for a health system on its path to achieving universal health coverage (UHC) and protects individuals from catastrophic health expenditures, especially in health emergencies. However, there are other dimensions to care access beyond financial accessibility. In this study, we assess the geographical accessibility of comprehensive emergency obstetric care (CEmOC) within the Lagos State Health Insurance Scheme.MethodsWe geocoded functional public and private CEmOC facilities, established facilities registered on the insurance panel as of December 2022, and assembled population distribution for women of childbearing age. We used Google Maps Platform's internal directions application programming interface to obtain driving times to facilities. State- and local government area (LGA)-level median travel time (MTT) and a number of CEmOC facilities reachable within 30 min were obtained for peak travel hours.ResultsAcross Lagos State, MTT to the nearest public CEmOC was 25 min, reduced to 17 min with private facilities added to the insurance panel. MTT to the nearest public facility in LGAs ranged from 9 min (Lagos Island) to 51 min (Ojo) (median = 25 min). With private facilities added, MTT ranged from 5 min (Agege and Ajeromi-Ifelodun) to 36 min (Ibeju-Lekki) (median = 13 min). On average, no public CEmOC facility was reachable within 30 min of driving for women living in 6 of 20 LGAs. With private facilities included in the scheme, reachable facilities within 30 min remained zero in one LGA (Ibeju-Lekki).ConclusionsOur innovative approach offers policy-relevant evidence to optimise insurance coverage, support efforts in advancing UHC, ensure coverage for CEmOC, and improve health system performance.
Molecular Mechanism of Mok I Gene Overexpression in Enhancing Monacolin K Production in Monascus pilosus
Authors: Huang Zhiwei , Xiao Lishi , Mo Wenlan , Zhang Yaru , Cai Yiyang , Huang Simei , Chen Zhiting , Long Chuannan 
Published: October 2024

Monascus species are capable of producing various active metabolites, including monacolin K (MK) and pigments. Studies have shown that the overexpression of the mok I gene from the MK synthesis gene cluster in Monascus species can significantly increase MK production; however, the molecular mechanism has not yet been fully elucidated. Therefore, this study focused on the mok I gene of Monascus pilosus to construct overexpression strains of the mok I gene, resulting in high-yield MK production. Sixteen positive transformants were obtained, seven of which produced 9.63% to 41.39% more MK than the original strain, with no citrinin detected in any of the transformants. The qRT-PCR results revealed that the expression levels of mok I in the transformed strains TI-13, TI-24, and TI-25 increased by more than 50% compared to the original strain at various fermentation times, with the highest increase being 10.9-fold. Furthermore, multi-omics techniques were used to analyze the molecular mechanisms underlying enhanced MK production in transformed strains. The results indicated that mok I overexpression may enhance MK synthesis in M. pilosus by regulating the expression of key genes (such as MAO, HPD, ACX, and PLC) and the synthesis levels of key metabolites (such as delta-tocopherol and alpha-linolenic acid) in pathways linked to the biosynthesis of cofactors, the biosynthesis of unsaturated fatty acids, tyrosine metabolism, ubiquinone and other terpenoid-quinone biosynthesis, alpha-linolenic acid metabolism, and glycerophospholipid metabolism. These findings provide a theoretical basis for further study of the metabolic regulation of MK in Monascus species and for effectively enhancing their MK production.
Анализ стратегических целей консолидации организаций в государственных корпорациях
Authors: Бакулина А.А. , Кандаурова Д.Ю. 
Published: October 2024

В статье анализируется консолидация бизнеса организаций, входящих в состав государственных корпораций. Актуальность исследования обусловлена необходимостью эффективного управления и развития государственных корпораций, так как они играют важную роль в экономическом развитии страны. В данной статье авторами проанализированы открытые источники данных, полученные с официальных сайтов госкорпораций (Государственная корпорация «ВЭБ.РФ», Государственная корпорация «Ростех», Агентство по страхованию вкладов (АСВ), Государственная корпорация по атомной энергии («Росатом») и др.). Авторы приходят к выводу, в Российской Федерации крупные отечественные компании и корпорации создали собственную тенденцию консолидации бизнеса, достигая поставленных в своей деятельности стратегических целей. Несмотря на достигнутые успехи в развитии формирования стратегии консолидации организаций в составе госкорпораций, многие вопросы нуждаются в актуализации и разработке нового инструментария, что еще раз подчеркивает своевременность и необходимость проведения исследования по обозначенной теме.
The article examines the consolidation of businesses within organizations that are part of State Corporations. The relevance of the study is driven by the need for effective management and development of state corporations, as they play a significant role in the country's economic growth. In this article, the author analyzes data from public sources obtained from the official websites of state corporations (State Corporation "VEB.RF", State Corporation "Rostec," Deposit Insurance Agency (DIA), State Atomic Energy Corporation "Rosatom," and others). The authors conclude that in the Russian Federation, large domestic companies and corporations have established their own trend of business consolidation, achieving the strategic goals set in their operations. Despite the successes in developing a consolidation strategy for organizations within state corporations, many issues require further refinement and the development of new tools, which underscores the timeliness and necessity of research on this topic.
Perfis sociodemográfico e clínico de indivíduos com Atrofia Muscular Espinhal e de seus cuidadores
Authors: Guimarães Melo Natália , Balçanufo de Souza e Silva Geovane , De Ávila Cecília Rosa , Aguilera Rodrigues da Silva Francine , Ferreira Martins Ribeiro Maysa , De Araújo Morais Letícia 
Published: October 2024

Objetivo. Analisar os perfis sociodemográfico e clínico de pessoas com Atrofia Muscular Espinhal (AME) e de seus cuidadores. Método. Estudo transversal, realizado em clínica de doenças neuromusculares de um centro de reabilitação. Foi utilizado um questionário com anamnese, identificação, perfis sociodemográfico e clínico. As variáveis categóricas foram apresentadas em frequência absoluta e relativa, e as variáveis contínuas pela média e desvio padrão. Para comparação entre os tipos de AME foram utilizados o Qui-quadrado de Pearson e a ANCOVA, nível de significância de p<0,05. Resultados. Participaram do estudo 27 cuidadores e 32 pessoas com AME, sendo 17 participantes com AME do tipo III, 9 do tipo II e 6 do tipo I. A idade dos participantes com AME variou entre 1 e 59 anos, com maior prevalência do sexo masculino (53,1%). No nível de escolaridade, as prevalências foram o ensino superior (31,3%) e o fundamental (28,1%). Apenas 34,4% tinham histórico de AME na família. Com relação aos aspectos clínicos, 87,5% necessitavam de dispositivo auxiliar para locomoção. O uso de medicação específica para AME e de suporte respiratório foi mais comum entre pessoas com AME dos tipos I e II. Quanto aos cuidadores, a maioria era composta por pessoas do sexo feminino (96,3%), mães (74,1%) e que se dedicavam exclusivamente ao lar e aos cuidados do indivíduo com AME (88,9%). Conclusão. A maioria dos participantes com AME não tinha histórico da doença na família e necessitava de dispositivo auxiliar para locomoção. As mães foram as cuidadoras prevalentes.
A Novel Role for FERM Domain-Containing Protein 3 (FRMD3) in CKD
Authors: Kennedy CiarÁn , Doyle Ross , Gough Oisin , Mcevoy Caitriona , Anallen Susan MC , Hughes Maria , Sheng Xin , Crifo Bianca , Andrews Darrell , Gaffney Andrew , Rodriguez Javier , Kennedy Susan , Dillon Eugene , Crean Daniel , Zhang Weijia , Yi Zhengzi , Nair Viji , Susztak Katalin , Hirschhorn Joel , Florez Jose , Groop Per-Henrik , Sandholm Niina , Kretzler Matthias , Mckay Gareth J. , Mcknight Amy Jayne , Maxwell Alexander P. , Matallanas David , Dorman Anthony , Martin Finian , Conlon Peter J. , Sadlier Denise M. ,   Genie Consortium, Brennan Eoin , Godson Catherine 
Published: October 2024

Background:
Currently there are limited methods to link disease severity and risk of disease progression in Chronic Kidney Disease (CKD). To better understand this potential relationship, we interrogated the renal transcriptomic profile of individuals with CKD with measures of CKD severity and identified FERM-domain containing protein 3 (FRMD3) as a candidate gene for follow-up study.

Methods:
RNA-seq was used to profile the transcriptome of CKD biopsies from the North Dublin Renal BioBank the results of which were correlated with clinical parameters. The potential function of FRMD3 was explored by interrogating the FRMD3 interactome and assessing the impact of lentiviral mediated FRMD3 knock down on human renal proximal tubule epithelial cells by assessing cell viability, metabolic activity, and structural markers.

Results:
We identified a subset of 93 genes which are significantly correlated with estimated glomerular filtration rate and percentage tubulointerstitial fibrosis at time of biopsy and with CKD progression 5 years post-biopsy. These results were validated against transcriptomic data from an external cohort of 432 nephrectomy samples. One of the top-ranking genes from this subset, FRMD3, has previously been associated with the risk of developing diabetic kidney disease. Interrogating the interactome of FRMD3 in tubule epithelial cells revealed interactions with cytoskeletal components of cell-cell junctions. Knockdown of FRMD3 expression in tubule epithelial cells resulted in increased pro-apoptotic activity within the cells as well as dysregulation of E-Cadherin.

Conclusions:
We have identified a panel of kidney-specific transcripts correlated with severity and progression of kidney disease, and from this have identified a possible role for FRMD3 in tubule cell structure and health.