bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radon, Secondhand Smoke, and Children in the Home: Creating a Teachable Moment for Lung Cancer Prevention
Authors: Huntington‐Moskos Luz , Rayens Mary Kay , Wiggins Amanda , Hahn Ellen J. 
Published: July 2016

AbstractObjectiveThis study determined whether having minor children in the home was associated with the teachable moment (TM) constructs of lung cancer worry, perceived risk, health‐related self‐concept, and the novel construct of synergistic risk.Design and SampleSecondary data analysis of baseline data from a randomized controlled trial of an intervention to reduce home exposure to radon and secondhand smoke (SHS). Quota sample of adults recruited at a Central Kentucky academic medical center (N = 556).MeasuresSurvey items assessed lung cancer worry, perceived risk, synergistic risk perception, and health‐related self‐concept.ResultsThe presence of children in the home was not a significant predictor of any construct needed to create a TM for lung cancer prevention. Individuals with children living in the home were more likely to be younger, a racial/ethnic minority, a current smoker, and live with a smoker compared to those without children in the home.ConclusionsThere is a critical need to raise parental awareness on child health inequities related to the home exposure to radon and SHS. Public health nurses can create TMs for lung cancer prevention through greater awareness of the risks posed by radon and SHS along with promoting home testing and low‐cost resources to reduce risk.
Public Health Nursing Practice in the Affordable Care Act Era: A National Survey
Authors: Edmonds Joyce K. , Campbell Lisa A. , Gilder Richard E. 
Published: July 2016

AbstractObjectivesTo explore public health nurses’ knowledge, perceptions, and practices under the Affordable Care Act (ACA).Design and SampleA cross‐sectional, web‐based survey was completed by a sample of 1,143 public health nurses (PHNs) in the United States.MeasuresDescriptive statistics were analyzed for variables related to general knowledge and perception of the ACA and for the extent of involvement in activities related to the implementation of the ACA. Qualitative analysis was conducted on free text comments to two open‐ended questions about current and future PHNs involvement in the ACA.ResultsApproximately 45% of PHNs reported changes in their daily work due to the ACA. PHNs reported being very or somewhat involved in these activities of the ACA: integration of primary care and public health (62%), provision of clinical preventive services (60.3%), care coordination (55.4%), patient navigation (55.3%), establishment of private‐public partnerships (55.3%), population health strategies (53.6%), population health data assessment and analysis (53.8%), community health assessments (49%), involvement in medical homes (37.8%), provision of maternal and child health home visiting services (32.1%), and involvement in Accountable Care Organizations (29.2%).ConclusionPHNs are making substantial contributions to implementation of the ACA.
Adaptive Isogeometric Digital Height Correlation: Application to Stretchable Electronics
Authors: Kleinendorst S. M. , Hoefnagels J. P. M. , Fleerakkers R. C. , van Maris M. P. F. H. L. , Cattarinuzzi E. , Verhoosel C. V. , Geers M. G. D. 
Published: July 2016

AbstractA novel adaptive isogeometric digital height correlation (DHC) technique has been developed in which the set of shape functions, needed for discretization of the ill‐posed DHC problem, is autonomously optimized for each specific set of profilometric height images, without a priori knowledge of the kinematics of the experiment. To this end, an adaptive refinement scheme is implemented, which refines the shape functions in a hierarchical manner. This technique ensures local refinement, only in the areas where needed, which is beneficial for the noise robustness of the DHC problem. The main advantage of the method is that it can be applied in experiments where the deformation mechanisms are unknown in advance, thereby complicating the choice of suitable shape functions. The method is applied to a virtual experiment in order to provide a proof of concept. A second virtual experiment is executed with stretchable electronics interconnects, which entail localized buckles upon deformation with complex kinematics. In both cases, accurate results were obtained, demonstrating the beneficial aspects of the proposed method. Moreover, the technique performance on profilometric images of a real experiment with stretchable interconnects was demonstrated.
The Vigna unguiculata Gene Expression Atlas (VuGEA) from de novo assembly and quantification of RNA‐seq data provides insights into seed maturation mechanisms
Authors: Yao Shaolun , Jiang Chuan , Huang Ziyue , Torres‐Jerez Ivone , Chang Junil , Zhang Heng , Udvardi Michael , Liu Renyi , Verdier Jerome 
Published: July 2016

SummaryLegume research and cultivar development are important for sustainable food production, especially of high‐protein seed. Thanks to the development of deep‐sequencing technologies, crop species have been taken to the front line, even without completion of their genome sequences. Black‐eyed pea (Vigna unguiculata) is a legume species widely grown in semi‐arid regions, which has high potential to provide stable seed protein production in a broad range of environments, including drought conditions. The black‐eyed pea reference genotype has been used to generate a gene expression atlas of the major plant tissues (i.e. leaf, root, stem, flower, pod and seed), with a developmental time series for pods and seeds. From these various organs, 27 cDNA libraries were generated and sequenced, resulting in more than one billion reads. Following filtering, these reads were de novo assembled into 36 529 transcript sequences that were annotated and quantified across the different tissues. A set of 24 866 unique transcript sequences, called Unigenes, was identified. All the information related to transcript identification, annotation and quantification were stored into a gene expression atlas webserver (http://vugea.noble.org), providing a user‐friendly interface and necessary tools to analyse transcript expression in black‐eyed pea organs and to compare data with other legume species. Using this gene expression atlas, we inferred details of molecular processes that are active during seed development, and identified key putative regulators of seed maturation. Additionally, we found evidence for conservation of regulatory mechanisms involving miRNA in plant tissues subjected to drought and seeds undergoing desiccation.
The effect of storage on ammonia, cytokine, and chemokine concentrations in feline whole blood
Authors: Cummings Katherine A. , Abelson Amanda L. , Rozanski Elizabeth A. , Sharp Claire R. 
Published: July 2016

AbstractObjectiveTo determine if the concentrations of ammonia and inflammatory mediators in feline stored whole blood (SWB) increase with duration of storage.DesignProspective ex vivo study.SettingUniversity Teaching Hospital.AnimalsThirteen cats, recruited from the hospital feline donor pool, deemed healthy based on the predonation donor screening process.InterventionsOne unit (30 mL) of whole blood was collected from 13 unique blood donor cats, anticoagulated with citrate‐phosphate‐dextrose, and stored at 4°C. Concentrations of ammonia, interleukin (IL) 6, and IL‐10 were measured in 5 units weekly for 4 weeks. Presence of chemokine ligand (CXCL) 8 was measured weekly in 8 other units in the same manner.Measurements and Main ResultsThe ammonia concentration increased nonlinearly with duration of storage, from a median of 48 μmol/L (range 25–74 μmol/L) on day 0 and 417 μmol/L (324–457 μmol/L) on day 28. IL‐6 and IL‐10 concentrations were below the lower limits of detection of the assay used (IL‐6 < 31.2 pg/mL and IL‐10 < 125 pg/mL). CXCL‐8 was detected in 4 of 8 SWB units at all time points.Conclusions and Clinical ImportanceAmmonia concentration increases with storage time in feline SWB. The clinical significance of this finding is yet to be determined. The presence of the proinflammatory chemokine CXCL‐8 in feline SWB warrants further research to determine whether it can incite an inflammatory response in the recipient. Further research evaluating the epidemiology of transfusion reactions in cats should evaluate the effect of unit age, and should include the possible impact of the presence of CXCL‐8.
The Anatolio‐Balkan phylogeographic fault: a snapshot from the genus Isophya (Orthoptera, Tettigoniidae)
Authors: Chobanov Dragan P. , Kaya Sarp , Grzywacz Beata , Warchałowska‐Śliwa Elżbieta , Çıplak Battal 
Published: July 2016

Due to active tectonic evolution of the Aegean Area during Miocene and Pleistocene, the Balkans and Anatolia have repeatedly connected and disconnected causing isolation and secondary contact of populations along the present Dardanelles – Sea of Marmara – Bosphorus waterway. This has led to an outstandingly rich fauna and a reticulate biogeography in the area. A typical example is Orthoptera having here possibly highest diversity within the Western Palaearctic. With the present study, we concentrate on the bush‐cricket genus Isophya, which is characteristic with a large share of endemics in the Balkans and Anatolia. We aim to understand when and how the isolation of marine or other barriers in the region of the Turkish Straits System influenced the evolution of the morpho‐acoustic groups of species found on both sides of the strait. For this purpose, sequences of two mitochondrial (COI and ND2) and two nuclear (ITS1 and ITS2) markers were obtained and used for phylogenetic reconstructions, time estimations for lineage divergence and automatic species delineation (statistical parsimony, GMYC, ABGD) tests. The phylogenetic analyses did not support all the existing morphogroups and was in favour of a geographical subdivision for the young lineages. Automatic species delineation tests confirmed most of the present morpho‐acoustic species and suggested further cryptic species, at the same time unifying some phenetic species. Time estimation analyses suggested time to most recent common ancestor of the genus as 8.28 Ma corresponding to the Late Tortonian. As a result of the study, we reached to the following conclusions: (i) most of the studied phenetic species are monophyletic, but some earlier suggested morpho‐species groups are not, (ii) three main episodes dominate the evolutionary history of Isophya (7.32–5.84 Myr in the Messinian, 2.2–2.5 Myr in the beginning of the Pleistocene and at around 0.75 Ma corresponding to the end of the Mid‐Pleistocene transition), all of which well correlate with episodes of existing terrestrial connections between Anatolia and the Balkans, and (iii) there are several faunal exchanges in both directions between Anatolia and the Balkans.
Complete Nucleotide Sequence of a French Isolate of
Maize rough dwarf virus
, a
Fijivirus
Member in the Family
Reoviridae
Authors: Svanella-Dumas L. , Marais A. , Faure C. , Theil S. , Thibord J. B. , Candresse T. 
Published: July 2016

ABSTRACT

The complete nucleotide sequence of a French isolate of
Maize rough dwarf virus
(MRDV) was determined by next-generation sequencing and compared with the single available complete sequence and with the partial sequences of two additional isolates available in online databases.
Draft Genome Sequence of a Tetrabromobisphenol A–Degrading Strain,
Ochrobactrum
sp. T, Isolated from an Electronic Waste Recycling Site
Authors: Liang Zhishu , Li Guiying , An Taicheng , Zhang Guoxia , Das Ranjit 
Published: July 2016

ABSTRACT

Ochrobactrum
sp. T was previously isolated from a sludge sample collected from an electronic waste recycling site and characterized as a unique tetrabromobisphenol A (TBBPA)–degrading bacterium. Here, the draft genome sequence (3.9 Mb) of
Ochrobactrum
sp. T is reported to provide insights into its diversity and its TBBPA biodegradation mechanism in polluted environments.
Genome Sequence of
Streptomyces
sp. Strain RTd22, an Endophyte of the Mexican Sunflower
Authors: Chagas Fernanda O. , Ruzzini Antonio C. , Bacha Larissa V. , Samborskyy Markyian , Conti Raphael , Pessotti Rita C. , de Oliveira Luciana G. , Clardy Jon , Pupo Monica T. 
Published: July 2016

ABSTRACT

We report here the complete genome sequence of
Streptomyces
sp. strain RTd22, an endophytic actinobacterium that was isolated from the roots of the Mexican sunflower
Tithonia diversifolia
. The bacterium's 11.1-Mb linear chromosome is predicted to encode a large number of unknown natural products.
Near-Complete Genome Sequence of Grapevine Fabavirus, a Novel Putative Member of the Genus
Fabavirus
Authors: Al Rwahnih Maher , Alabi Olufemi J. , Westrick Nathaniel M. , Golino Deborah , Rowhani Adib 
Published: July 2016

ABSTRACT

A novel virus-like sequence from grapevine was identified by Illumina sequencing. The genomic organization was most similar to that of members of the genus
Fabavirus
. Polyproteins RNA-1 and RNA-2 of the virus tentatively named grapevine fabavirus (GFabV) shared 34 to 23% sequence identities with
Broad bean wilt virus 2
(BBWV2), respectively. GFabV was successfully graft transmitted to
Vitis vinifera
cv. Cabernet Franc.
Complete Genome Sequence of
Psychrobacter alimentarius
PAMC 27889, a Psychrophile Isolated from an Antarctic Rock Sample
Authors: Lee Jaejin , Kwon Miye , Yang Jae Young , Woo Jusun , Lee Hong Kum , Hong Soon Gyu , Kim Ok-Sun 
Published: July 2016

ABSTRACT

Psychrobacter alimentarius
PAMC 27889, a Gram-negative, psychrophilic bacterium, was isolated from an Antarctic rock sample. Here, we report the complete genome of
P. alimentarius
PAMC 27889, which has the nonmevalonate methylerythritol phosphate pathway of terpenoid biosynthesis and a complete gene cluster for benzoate degradation.
Draft Genome Sequence of
Clostridium difficile
Strain IT1118, an Epidemic Isolate Belonging to the Emerging PCR Ribotype 018
Authors: Wasels François , Barbanti Fabrizio , Spigaglia Patrizia 
Published: July 2016

ABSTRACT

Clostridium difficile
PCR ribotype 018 has emerged in Italy, South Korea, and Japan, causing severe infections and outbreaks. In this study, we sequenced the genome of IT1118, an Italian clinical isolate, to clarify the molecular features contributing to the success of this epidemic type.
BCG vaccination at birth and early childhood hospitalisation: a randomised clinical multicentre trial
Authors: Stensballe Lone Graff , Sørup Signe , Aaby Peter , Benn Christine Stabell , Greisen Gorm , Jeppesen Dorthe Lisbeth , Birk Nina Marie , Kjærgaard Jesper , Nissen Thomas Nørrelykke , Pihl Gitte Thybo , Thøstesen Lisbeth Marianne , Kofoed Poul-Erik , Pryds Ole , Ravn Henrik 
Published: July 2016

BackgroundThe BCG vaccine is administered to protect against tuberculosis, but studies suggest there may also be non-specific beneficial effects upon the infant immune system, reducing early non-targeted infections and atopic diseases. The present randomised trial tested the hypothesis that BCG vaccination at birth would reduce early childhood hospitalisation in Denmark, a high-income setting.MethodsPregnant women planning to give birth at three Danish hospitals were invited to participate. After parental consent, newborn children were allocated to BCG or no intervention within 7 days of age. Randomisation was stratified by prematurity. The primary study outcome was number of all-cause hospitalisations analysed as repeated events. Hospitalisations were identified using The Danish National Patient Register. Data were analysed by Cox proportional hazards models in intention-to-treat and per-protocol analyses.Results4184 pregnant women were randomised and their 4262 children allocated to BCG or no intervention. There was no difference in risk of hospitalisation up to 15 months of age; 2129 children randomised to BCG experienced 1047 hospitalisations with a mean of 0.49 hospitalisation per child compared with 1003 hospitalisations among 2133 control children (mean 0.47), resulting in a HR comparing BCG versus no BCG of 1.05 (95% CI 0.93 to 1.18) (intention-to-treat analysis). The effect of BCG was the same in children born at term (1.05 (0.92 to 1.18)) and prematurely (1.07 (0.63 to 1.81), p=0.94). The effect was also similar in the two sexes and across study sites. The results were essentially identical in the per-protocol analysis and after adjustment for baseline characteristics.ConclusionsBCG vaccination at birth did not reduce the risk of hospitalisation for somatic acquired disease until 15 months of age in this Danish study population.Trial registration numberNCT01694108, results.
Effect of exercise training on C reactive protein: a systematic review and meta-analysis of randomised and non-randomised controlled trials
Authors: Fedewa Michael V , Hathaway Elizabeth D , Ward-Ritacco Christie L 
Published: July 2016

PurposeC-reactive protein (CRP) is a marker of chronic systemic inflammation frequently used in cardiovascular disease risk assessment. The purpose of this meta-analysis was to provide a quantitative estimate of the magnitude of change in CRP following participation in physical exercise interventions.MethodsAll studies included in the meta-analysis were peer reviewed and published in English. Human participants were assigned to a non-exercise comparison group or exercise training group, with the intervention lasting ≥2 weeks. CRP levels were measured at baseline, during and/or after completion of the exercise training programme. Random-effects models were used to aggregate a mean effect size (ES), 95% CIs and potential moderators.Results83 randomised and non-randomised controlled trials met the inclusion criteria and resulted in 143 effects (n=3769). The mean ES of 0.26 (95% CI 0.18 to 0.34, p<0.001) indicated a decrease in CRP following exercise training. A decrease in body mass index (BMI; β=1.20, SE=0.25, p<0.0001) and %Fat (β=0.76, SE=0.21, p=0.0002) were associated with a decrease in CRP, independently accounting for 11.1% and 6.6% of the variation in response, respectively. Exercise training led to a greater reduction in CRP when accompanied by a decrease in BMI (ES=0.38, 95% CI 0.26 to 0.50); however, a significant improvement in CRP occurred in the absence of weight loss (ES=0.19, 95% CI 0.10 to 0.28; both p<0.001).ConclusionsThese results suggest that engaging in exercise training is associated with a decrease in CRP levels regardless of the age or sex of the individual; however, greater improvements in CRP level occur with a decrease in BMI or %Fat.
Distinguishing the associations between daily mortality and hospital admissions and nitrogen dioxide from those of particulate matter: a systematic review and meta-analysis
Authors: Mills I C , Atkinson R W , Anderson H R , Maynard R L , Strachan D P 
Published: July 2016

ObjectivesTo quantitatively assess time-series studies of daily nitrogen dioxide (NO2) and mortality and hospital admissions which also controlled for particulate matter (PM) to determine whether or to what extent the NO2associations are independent of PM.DesignA systematic review and meta-analysis.MethodsTime-series studies—published in peer-reviewed journals worldwide, up to May 2011—that reported both single-pollutant and two-pollutant model estimates for NO2and PM were ascertained from bibliographic databases (PubMed, EMBASE and Web of Science) and reviews. Random-effects summary estimates were calculated globally and stratified by different geographical regions, and effect modification was investigated.Outcome measuresMortality and hospital admissions for various cardiovascular or respiratory diseases in different age groups in the general population.Results60 eligible studies were identified, and meta-analysis was conducted on 23 outcomes. Two-pollutant model study estimates generally showed that the NO2associations were independent of PM mass. For all-cause mortality, a 10 µg/m3increase in 24-hour NO2was associated with a 0.78% (95% CI 0.47% to 1.09%) increase in the risk of death, which reduced to 0.60% (0.33% to 0.87%) after control for PM. Heterogeneity between geographical region-specific estimates was removed by control for PM (I2from 66.9% to 0%). Estimates of PM and daily mortality assembled from the same studies were greatly attenuated after control for NO2: from 0.51% (0.29% to 0.74%) to 0.18% (−0.11% to 0.47%) per 10 µg/m3PM10and 0.74% (0.34% to 1.14%) to 0.54% (−0.25% to 1.34%) for PM2.5.ConclusionsThe association between short-term exposure to NO2and adverse health outcomes is largely independent of PM mass. Further studies should attempt to investigate whether this is a generic PM effect or whether it is modified by the source and physicochemical characteristics of PM. This finding strengthens the argument for NO2having a causal role in health effects.
Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis
Authors: Aarup Annemarie , Pedersen Tanja X. , Junker Nanna , Christoffersen Christina , Bartels Emil D. , Madsen Marie , Nielsen Carsten H. , Nielsen Lars B. 
Published: July 2016

Objective—
Atherosclerotic lesions contain hypoxic areas, but the pathophysiological importance of hypoxia is unknown. Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor in cellular responses to hypoxia. We investigated the hypothesis that HIF-1α has effects on macrophage biology that promotes atherogenesis in mice.

Approach and Results—

Studies with molecular probes, immunostaining, and laser microdissection of aortas revealed abundant hypoxic, HIF-1α–expressing macrophages in murine atherosclerotic lesions. To investigate the significance of macrophage HIF-1α,
Ldlr
−/−
mice were transplanted with bone marrow from mice with HIF-1α deficiency in the myeloid cells or control bone marrow. The HIF-1α deficiency in myeloid cells reduced atherosclerosis in aorta of the
Ldlr
−/−
recipient mice by ≈72% (
P
=0.006).

In vitro, HIF-1α–deficient macrophages displayed decreased differentiation to proinflammatory M1 macrophages and reduced expression of inflammatory genes. HIF-1α deficiency also affected glucose uptake, apoptosis, and migratory abilities of the macrophages.

Conclusions—
HIF-1α expression in macrophages affects their intrinsic inflammatory profile and promotes development of atherosclerosis.