bluuebunny/crossref_metadata_2025_split huggingface dataset:
Living microorganisms change the information (Shannon) content of a geophysical system
Authors: Tang Fiona H. M. , Maggi Federico 
Published: June 2017

AbstractThe detection of microbial colonization in geophysical systems is becoming of interest in various disciplines of Earth and planetary sciences, including microbial ecology, biogeochemistry, geomicrobiology, and astrobiology. Microorganisms are often observed to colonize mineral surfaces, modify the reactivity of minerals either through the attachment of their own biomass or the glueing of mineral particles with their mucilaginous metabolites, and alter both the physical and chemical components of a geophysical system. Here, we hypothesise that microorganisms engineer their habitat, causing a substantial change to the information content embedded in geophysical measures (e.g., particle size and space-filling capacity). After proving this hypothesis, we introduce and test a systematic method that exploits this change in information content to detect microbial colonization in geophysical systems. Effectiveness and robustness of this method are tested using a mineral sediment suspension as a model geophysical system; tests are carried out against 105 experiments conducted with different suspension types (i.e., pure mineral and microbially-colonized) subject to different abiotic conditions, including various nutrient and mineral concentrations, and different background entropy production rates. Results reveal that this method can systematically detect microbial colonization with less than 10% error in geophysical systems with low-entropy background production rate.
Identification of novel dysregulated key genes in Breast cancer through high throughput ChIP-Seq data analysis
Authors: Raj Utkarsh , Aier Imlimaong , Semwal Rahul , Varadwaj Pritish Kumar 
Published: June 2017

AbstractBreast cancer is the most common cancer in women both in the developed and less developed countries, and it imposes a considerable threat to human health. Therefore, in order to develop effective targeted therapies against Breast cancer, a deep understanding of its underlying molecular mechanisms is required. The application of deep transcriptional sequencing has been found to be reported to provide an efficient genomic assay to delve into the insights of the diseases and may prove to be useful in the study of Breast cancer. In this study, ChIP-Seq data for normal samples and Breast cancer were compared, and differential peaks identified, based upon fold enrichment (with P-values obtained via t-tests). The Protein–protein interaction (PPI) network analysis was carried out, following which the highly connected genes were screened and studied, and the most promising ones were selected. Biological pathway involved in the process were then identified. Our findings regarding potential Breast cancer-related genes enhances the understanding of the disease and provides prognostic information in addition to standard tumor prognostic factors for future research.
Morphology controls how hippocampal CA1 pyramidal neuron responds to uniform electric fields: a biophysical modeling study
Authors: Yi Guo-Sheng , Wang Jiang , Deng Bin , Wei Xi-Le 
Published: June 2017

AbstractResponses of different neurons to electric field (EF) are highly variable, which depends on intrinsic properties of cell type. Here we use multi-compartmental biophysical models to investigate how morphologic features affect EF-induced responses in hippocampal CA1 pyramidal neurons. We find that the basic morphologies of neuronal elements, including diameter, length, bend, branch, and axon terminals, are all correlated with somatic depolarization through altering the current sources or sinks created by applied field. Varying them alters the EF threshold for triggering action potentials (APs), and then determines cell sensitivity to suprathreshold field. Introducing excitatory postsynaptic potential increases cell excitability and reduces morphology-dependent EF firing threshold. It is also shown that applying identical subthreshold EF results in distinct polarizations on cell membrane with different realistic morphologies. These findings shed light on the crucial role of morphologies in determining field-induced neural response from the point of view of biophysical models. The predictions are conducive to better understanding the variability in modulatory effects of EF stimulation at the cellular level, which could also aid the interpretations of how applied fields activate central nervous system neurons and affect relevant circuits.
Zinc finger gene 217 (ZNF217) Promoted Ovarian Hyperstimulation Syndrome (OHSS) through Regulating E2 Synthesis and Inhibiting Thrombospondin-1 (TSP-1)
Authors: Zhai Junyu , Liu Jiansheng , Cheng Xiaoyue , Li Shang , Hong Yan , Sun Kang , Chen Zi-Jiang , Du Yanzhi , Li Weiping 
Published: June 2017

AbstractZinc finger gene 217 (ZNF217) is a candidate gene of polycystic ovary syndrome (PCOS) which is vulnerable to ovarian hyperstimulation syndrome (OHSS). However, the relationship between ZNF217 and OHSS is largely unknown. Our study demonstrated that ZNF217 was mainly distributed in the granulosa cells of rat ovary. Significantly higher expression of ovarian ZNF217 was detected in OHSS rats, being consistent with serum 17β-estradiol concentration and ovarian aromatase. Moreover, OHSS rats also showed decreased ovarian TSP-1 mRNA, an acknowledged VEGF signaling suppressor. The same changes were detected in human granulosa cells and follicular fluid. Thus, the increased ZNF217 and decreased TSP-1 may participate in OHSS onset. In vitro experiment revealed that ZNF217 positively regulated E2 synthesis through promoting cAMP response element binding protein (CREB) and thereby CYP19A1 in KGN cells. Furthermore, ZNF217 negatively regulated TSP-1 in KGN cells while TSP-1 promoted claudin1 and inhibited nitric oxide (NO) in HUVECs and HAECs. Both of claudin1 and NO are responsible for the regulation of vascular permeability (VP). Therefore, we demonstrated that ZNF217 contributed to OHSS onset through promoting E2 synthesis and the increase of VP. Moreover, the increased ZNF217 and decreased TSP-1 provided new targets for the prevention or treatment of OHSS in the future.
Methylation of DACT2 promotes breast cancer development by activating Wnt signaling
Authors: Li Jingyi , Zhang Meiying , He Tao , Li Hongxia , Cao Tingting , Zheng Lili , Guo Mingzhou 
Published: June 2017

AbstractBreast cancer is the most common malignant tumor in women worldwide. To explore the role of DACT2 in breast cancer, 5 cell lines and 153 cases of primary cancer were studied. The expression of DACT2 was detected in BT474, MDA-MB-231 and BT549 cells, while no expression was found in MDA-MB-468 and HBL100 cells. Complete methylation of DACT2 was found in MDA-MB-468 and HBL100 cells, partial methylation was observed in BT474 and BT549 cells, and no methylation was detected in MDA-MB-231 cells. Restoration of DACT2 expression was induced by 5-Aza in MDA-MB-468 and HBL100 cells. DACT2 was methylated in 49.7% (76/153) of primary breast cancer samples. Methylation of DACT2 was significantly associated with tumor size (P < 0.05). Reduced DACT2 expression was significantly associated with promoter region methylation in primary breast cancer (P < 0.05). DACT2 suppressed breast cancer cell growth and induced G1/S phase arrest in breast cancer cells. DACT2 inhibited Wnt/β-catenin signaling in human breast cancer cells and suppressed breast cancer cell tumor growth in xenograft mice. In conclusion, our results demonstrate that DACT2 is frequently methylated in human breast cancer, methylation of DACT2 activates Wnt signaling, and DACT2 suppresses breast cancer cell growth both in vitro and in vivo.
GABAA receptor subunit gene polymorphisms predict symptom-based and developmental deficits in Chinese Han children and adolescents with autistic spectrum disorders
Authors: Yang Shuhan , Guo Xuan , Dong Xiaopeng , Han Yu , Gao Lei , Su Yuanyuan , Dai Wei , Zhang Xin 
Published: June 2017

AbstractGABAA receptor subunit genes GABRB3, GABRA5, and GABRG3 located on chromosome 15q11-q13 have been implicated in the etiology of autistic spectrum disorders (ASD). This study intended to investigate the possible role of single-nucleotide polymorphisms (SNPs) present in GABRB3 (rs2081648 and rs1426217), GABRA5 (rs35586628), and GABRG3 (rs208129) genes in ASD susceptibility and symptom-based and developmental phenotypes of ASD in Chinese Han children and adolescents. 99 ASD patients and 231 age- and gender- frequency-matched typical developing (TD) controls were tested by TaqMan® genotyping assay. Symptom-based phenotypes were evaluated by Childhood Autism Rating Scale (CARS) and Autism Behavior Checklist (ABC), and developmental phenotypes were assessed by Early Childhood Development Questionnaire (ECDQ) in ASD patients. Three haplotypes and global χ
2
test of all SNPs demonstrated significant associations between ASD and TD groups. Besides, GABRB3 rs2081648, GABRA5 rs35586628, and GABRG3 rs208129 polymorphisms were associated with symptom-based deficits in social interaction, sensorimotor and somatosensory coordination, visual response, imitation, activity level, language expression and adaptability. Developmental abnormalities in late emergences of social interaction and fine motor were detected in GABRB3 rs2081648 polymorphism. Overall results indicated that gene synergy may participate in ASD pathogenesis, and GABAA receptor gene polymorphisms can predict symptom-based and developmental deficits in ASD individuals.
Noncomprehension Signaling in Males and Females With Fragile X Syndrome
Authors: Thurman Angela John , Kover Sara T. , Ted Brown W. , Harvey Danielle J. , Abbeduto Leonard 
Published: June 2017

Purpose
This study used a prospective longitudinal design to evaluate the trajectory and predictors of noncomprehension signaling in male and female youth with fragile X syndrome (FXS).

Method
A direction-following task in which some of the directions were inadequate was administered. Participants were 52 youth (36 boys, 16 girls) with FXS. Upon study entry, participants ranged from 10 to 16 years. The average number of annual assessments per participant was 3.65 (range = 1–4), providing 198 data points for analysis.

Results
Participants with FXS were less likely to signal noncomprehension than younger, typically developing, cognitively matched children. The average rate of change in noncomprehension signaling was not significantly different from 0 for either boys or girls, suggesting a plateau. Both FMRP and nonverbal IQ were significant independent predictors of noncomprehension signaling for boys. Variability in noncomprehension signaling among girls was not explained by any of the predictors, but trends similar to those observed for boys were observed.

Conclusions
Noncomprehension signaling appears to be an area of weakness for individuals with FXS. Because the failure to signal noncomprehension can have negative, cumulative effects on comprehension, the results suggest a need for interventions targeting the requisite cognitive skills.
A Case of Pituitary Carcinoma Initially Diagnosed as an Ectopic Growth Hormone Producing Pituitary Adenoma with a High Ki-67 Labeling Index
Authors: Endo Toshiki , Watanabe Mika , Tominaga Teiji , Ogawa Yoshikazu 
Published: June 2017

AbstractWe report a case of a 66-year-old woman with a malignant transformation of a growth hormone (GH)-producing pituitary adenoma that was mainly located in the clivus. The patient originally presented with left oculomotor and abducens nerve palsies. Magnetic resonance imaging revealed a clival tumor separated from the normal pituitary gland, and the patient subsequently underwent transsphenoidal surgery. Analysis of the resected clival tissue revealed proliferation of tumor cells with slightly irregular nuclei but no mitosis. Although the Ki-67 labeling index was as high as 8.7%, p53 was negative. Histologic analysis confirmed the diagnosis of a GH-producing pituitary adenoma. Two months after the first operation, the tumor acutely enlarged and caused a subarachnoid hemorrhage. Pathologic findings of the second surgical specimen showed significant nuclear atypia. The Ki-67 labeling index increased to 27.7% and the p53 was positive; there was no GH immunoreactivity. Following the second surgical intervention, the patient was diagnosed with an atypical pituitary adenoma and underwent postoperative local radiotherapy (50 Gy in 25 fractions). Twenty months after the first surgery, multiple bone metastases were detected that led to the diagnosis of pituitary carcinoma. This is the first report of a clival pituitary adenoma undergoing malignant transformation. Because neuroimaging may underestimate local invasiveness in ectopic pituitary tumors, it is essential to conduct a histologic examination to evaluate it. Further analysis of similar cases is necessary to improve clinical management for this confused diagnostic criterion.
Completely Thrombosed Aneurysms of the Posterior Cerebral Artery: A Comprehensive Review
Authors: Cuartero Pérez Beatriz , Ros de San Pedro Javier 
Published: June 2017

Background Completely thrombosed aneurysms of the posterior cerebral artery (CTPCAAs) are rare lesions with different characteristics and clinical behavior when compared with other intracranial aneurysms.
Objective To describe and analyze the main features of CTPCAAs.
Material and Methods Fifteen CTPCAAs were studied (14 literature cases plus one illustrative case). Clinical, radiologic, pathologic, and therapeutic data were obtained and analyzed.
Results CTPCAAs are large (mean: 2.43 cm), located proximally (80% at P1–P2 segments), and show a slight predominance of a fusiform appearance. Posterior cerebral artery (PCA) occlusion ensued after aneurysm complete thrombosis in 73% of patients (11/15). However, only 33% of patients developed a PCA territory stroke, due to a rich distal arterial collateral network. Three mutually exclusive clinical presentations were observed: subarachnoid hemorrhage (SAH) (n = 3), “stroke-like” syndrome (n = 7), and “tumor-like” syndrome (n = 4). One case was incidental. Three treatments were performed: conservative (n = 7), endovascular (n = 1), and surgery (n = 7). CTPCAA exclusion was the goal of surgery. Neural structures decompression was also sought in tumor-like cases. CTPCAA elimination as a potential bleeding source was the target in stroke-like cases. General outcome among CTPCAAs was good (73% of cases scored Glasgow Outcome Scale 4–5/Modified Rankin Scale 0–2).
Conclusions CTPCAAs are characterized by young age at presentation, male predominance, proximal location on PCA, and tendency to cause PCA occlusion. The PCAA clinical presentation depends on the mechanism of complete thrombosis. SAH and stroke-like syndrome CTPCAAs harbor a less organized thrombus than tumor-like CTPCAAs, which makes the aneurysm more prone to hemorrhagic/ischemic complications. Thus clinical presentation may indicate the clinical evolution in CTPCAAs. The management of CTPCAAs varied according to the patient's clinical condition and the risk evaluation for future complications derived from the aneurysm. Clinical surveillance and surgical removal were the most frequent treatment options performed. Surgery was focused on neural decompression and/or CTPCAA elimination as a potential source of bleeding.
Elternschaft, Partnerschaft und familiäre Konstellationen bei Methamphetaminkonsumierenden – Eine Analyse der Beratungsdokumentation in der ambulanten Suchthilfe
Authors: Dyba Janina , Klein Michael , Wetzel Wolfgang 
Published: June 2017

Zusammenfassung
Ziel der Studie Die Studie beschreibt die familiäre Situation Methamphetaminkonsumierender in der ambulanten Suchtberatung mit speziellem Fokus auf Konstellationen von Elternschaft und Partnerschaft.
Methoden Es wurde ein Dokumentenanalysebogen entwickelt, anhand dessen die Fallakten der Jahre 2013 und 2014 von Methamphetamin konsumierenden Eltern in der ambulanten Suchtberatungsstelle in Zwickau anonymisiert analysiert wurden. Fokussiert wurden dabei neben soziodemografischen und konsumbezogenen Angaben, besonders Informationen zu Partnerschaft und Kindern.
Ergebnisse In den Familien finden sich häufig junge Kinder, welche in der Regel (zumindest zeitweise) nicht beim konsumierenden Elternteil aufwachsen. Partnerschaften zeigen sich fragil und geprägt vom Substanzgebrauch beider Partner.
Schlussfolgerung Die Familiensysteme offenbaren multiple Problemlagen und sind vielmals geprägt von Diskontinuität und Instabilität, wobei sich erhebliche Entwicklungsrisiken für die Kinder abzeichnen.
Osteopathische Behandlungen für das neurovegetative
System
Authors: Vogt Ralf 
Published: June 2017

ZusammenfassungDas vegetative Nervensystem stellt als übergeordnetes System der Körpersteuerung
eine besondere Herausforderung für die osteopathische Behandlung dar. Es sprengt
den Rahmen der üblichen Betrachtungsweise, bei der oft in parietal, viszeral und
kraniosakral unterteilt wird. Wir können den Körper nur in seiner Ganzheit
behandeln, indem wir versuchen, seine neurovegetative Autoregulation über
Strukturen des Nervensystems, des endokrinen Systems und der alles begleitenden
Fasziensysteme zu aktivieren. Dies erfordert auch bei den verwendeten Techniken
einen übergreifenden Ansatz.
Leave it alone, and then ...?
Authors: Stark Jane 
Published: June 2017

ZusammenfassungFinde die Läsion, passe sie an und überlasse den Rest der Physiologie des Körpers
– so könnte man den bekannten, Andrew Taylor Still zugeschriebenen Leitspruch
„Find it, fix it and leave it alone“ der Osteopathie ins Deutsche übersetzen.
Doch stammt diese Aussage tatsächlich von Still, und wie zeitgemäß ist sie?
Moderne Interpretationen zeigen: Im Zentrum von Stills Aussage standen das
physiologische System des Menschen und die Selbstorganisationskräfte der
Natur.
Knieschmerz aus viszeralosteopathischer Sicht
Authors: Bonacker Michael 
Published: June 2017

ZusammenfassungEin 14-jähriges Mädchen kommt wegen Knieschmerzen zu mir in die Praxis. Interessanterweise lösten bisherige orthopädische und
physiotherapeutische Maßnahmen nicht ansatzweise ihr Problem. Dies hatte aber für mich den Vorteil, dass ich möglicherweise eine parietale
Kausalität ausschließen konnte. Eine ausführliche Anamnese und körperliche Untersuchung in Verbindung mit dem Background der neuralen
Vernetzung der Becken-Bauch-Region zur unteren Extremität brachten mich auf eine außergewöhnliche Idee.
Keratin, Kollagen oder doch Spendergewebe – wo liegt die Zukunft in der Entwicklung neuer Biomaterialien zur Hornhautrekonstruktion?
Authors: Bachmann Björn , Schrader Stefan 
Published: June 2017

ZusammenfassungFür den Ersatz von Hornhautgewebe werden seit Langem standardmäßig Hornhauttransplantate bzw. Amnionmembran verwendet. Da es sich hierbei um biologisches Gewebe handelt, besteht nur eine eingeschränkte Standardisierung, was die Herstellung, Beschaffenheit und die Eigenschaften nach Transplantation betrifft. Darüber hinaus gibt es ein Risiko der Krankheitsübertragung vom Spender und die Verfügbarkeit sowohl von menschlichen Hornhäuten als auch von Amnionmembran ist in vielen Regionen der Erde ungenügend. Aus diesem Grund werden seit vielen Jahren alternative Biomaterialien für den Hornhautersatz beforscht. Unter den natürlichen Biomaterialien bieten Materialien auf Kollagen- oder Keratinbasis Eigenschaften, die sie zu aussichtsreichen Kandidaten für den Hornhautstromaersatz machen. Aktuell bestehen aber noch viele ungelöste Probleme, insbesondere was die Degradation nach Implantation und die Nahtfestigkeit der Materialien angeht. Erste klinische Untersuchungen mit unterschiedlichen Biomaterialien auf Kollagenbasis belegen jedoch ihre insgesamt recht gute Biokompatibilität hinsichtlich ihrer Integrationsfähigkeit bzw. hinsichtlich ihrer geringen Immunogenität. Aktuell gibt es kein Biomaterial, das den Anforderungen in jeder Situation gerecht wird. Es ist zu vermuten, dass zukünftig unterschiedliche Biomaterialen zur Verfügung stehen, die in Abhängigkeit von der zugrunde liegenden Hornhauterkrankung unterschiedliche Funktionen erfüllen und so eine patienten- und krankheitsindividuelle Versorgung möglich machen.
Erstellung eines Handlungsleitfadens für einen gesundheitsförderlichen Umgang mit Informations- und Kommunikationstechnologien, Flexibilisierung und Erreichbarkeit in kleinen und mittleren Unternehmen
Authors: Weilnhammer Veronika , Heinze Stefanie , Heiden Barbara , Palm Esther , Herbig Britta , Lüke Gabriele , Nowak Dennis , Glaser Jürgen , Herr Caroline 
Published: June 2017

Zusammenfassung
Hintergrund Innerhalb der letzten Jahrzehnte hat sich die Arbeitswelt stark gewandelt. Neue Informations- und Kommunikationstechnologien (IKT) machen es vielfach möglich, zu jeder Zeit an jedem Ort zu arbeiten. Flexibilisierung, erweiterte Erreichbarkeit und Entgrenzung von Arbeit und Privatleben können möglicherweise daraus resultieren. Die Verbreitung von IKT und mögliche Auswirkungen dieser Entwicklung auf die Gesundheit und das Wohlbefinden betroffener Mitarbeiter wurden bisher v. a. in großen Unternehmen untersucht. Kleine und mittlere Unternehmen (KMU) sind in diesem Zusammenhang selten Gegenstand wissenschaftlicher Studien.
Ziel Ziel dieser Arbeit war es daher, anhand der Erfahrungen und Ergebnissen einer Studie zu Flexibilisierung in der Arbeitswelt (FlexA) sowie einer systematischen Literaturrecherche einen Handlungsleitfaden (HLF) zu entwickeln. Dieser richtet sich an KMU mit dem Ziel, einen gesundheitsförderlichen Umgang mit IKT zu erarbeiten und in die Unternehmenskultur zu integrieren.
Methodik Um den Stand der Forschung zu IKT-bezogenen Interventionen abzubilden, wurde eine systematische Literaturrecherche in verschiedenen Datenbanken, z. B. PsycINFO, EconLit und Medline durchgeführt. Dazu wurden u. a. die Begriffe „Arbeit“, „Intervention“, „IKT“ verwendet und in eine Suchstrategie überführt. Zusätzlich wurden graue Literatur und die Ergebnisse des FlexA Projektes zur Erstellung des HLF einbezogen.
Ergebnisse Von den 30 Treffern der systematischen Literaturrecherche konnte, nach Screening von Titel und Abstract anhand festgelegter Ausschlusskriterien, keine Arbeit eingeschlossen werden. Aus grauer Literatur konnten 4 Veröffentlichungen als relevant identifiziert werden. Die in den Unternehmensworkshops des FlexA Projektes erarbeiteten Maßnahmen, wie z. B. das Einführen von kollektiven Regelungen in den Betrieben, wurden in den HLF aufgenommen.
Schlussfolgerung Aufgrund der permanenten Weiterentwicklung von IKT und den damit verbundenen Veränderungen in Bezug auf die Flexibilisierung in der Arbeitswelt, wird es immer wichtiger werden, diese Entwicklung zu begleiten, aktiv und gesundheitsförderlich zu gestalten und innerhalb des jeweiligen Unternehmens in geregelten Bahnen stattfinden zulassen. Der vorliegende Leitfaden soll Unternehmer bei dieser Aufgabe unterstützen und ist ein wichtiges Instrument für den Umgang mit IKT hinsichtlich Flexibilisierung und Erreichbarkeit im Arbeitsalltag.