bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of contrast‐enhanced ultrasonograpy with Gd‐EOB‐DTPA‐enhanced MRI in the diagnosis of liver metastasis from colorectal cancer
Authors: Shiozawa Kazue , Watanabe Manabu , Ikehara Takashi , Matsukiyo Yasushi , Kogame Michio , Kikuchi Yoshinori , Otsuka Yuichiro , Kaneko Hironori , Igarashi Yoshinori , Sumino Yasukiyo 
Published: November 2016

ABSTRACTPurposeTo compare contrast‐enhanced ultrasonography (CEUS) using Sonazoid with Gd‐EOB‐DTPA‐enhanced MRI (EOB‐MRI) in the diagnosis of liver metastases in patients with colorectal cancer.MethodsA total of 69 patients diagnosed with or suspected of having liver metastasis were enrolled. These hepatic lesions were diagnosed by histopathological examination after surgical resection or based on follow‐up using various imaging modalities. The diagnostic accuracies of CEUS and EOB‐MRI were compared.ResultsOne hundred thirty‐three lesions were detected. Of these lesions, 109 were diagnosed as liver metastases. Of the 133 lesions, 90.2% were detected on CEUS, and 98.5% on EOB‐MRI. One hundred nine lesions were diagnosed as liver metastasis. The areas under the receiver operating characteristic curve for diagnosis were 0.906 and 0.851 on CEUS and EOB‐MRI, respectively (p = 0.41). Sensitivity, specificity, positive predictive value (PPV), negative predictive value, and overall accuracy were 90.8%, 84.5%, 97.1%, 67.1%, and 90.2%, respectively, for CEUS, and 95.4%, 70.8%, 93.7%, 77.3%, and 91%, respectively, for EOB‐MRI.ConclusionsCEUS has a higher specificity and PPV for the diagnosis of liver metastasis than EOB‐MRI. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 45:138–144, 2017
Efficient synthesis of [18F]FPyME: A new approach for the preparation of maleimide‐containing prosthetic groups for the conjugation with thiols
Authors: Cavani Melanie , Bier Dirk , Holschbach Marcus , Coenen Heinz H. 
Published: November 2016

AbstractAn improved high yielding radiosynthesis of the known thiol‐reactive maleimide‐containing prosthetic group1‐[3‐(2‐[18F]fluoropyridine‐3‐yloxy)propyl]pyrrole‐2,5‐dione ([18F]FPyME) is described. The target compound was obtained by a two‐step one‐pot procedure starting from a maleimide‐containing nitro‐precursor that was protected as a Diels‐Alder adduct with 2,5‐dimethylfurane. Nucleophilic radiofluorination followed by heat induced deprotection through a Retro Diels Alder reaction yielded, after chromatographic isolation, [18F]FPyME with a radiochemical yield of 20% in about 60 min overall synthesis time. A variety of other [18F]fluoropyridine based maleimide‐containing prosthetic groups should be accessible via the described synthetic strategy.
Structural and molecular analysis of a protective epitope of Lyme disease antigen OspA and antibody interactions
Authors: Shandilya Shivender , Kurt Yilmaz Nese , Sadowski Andrew , Monir Ejemel , Schiller Zachary A. , Thomas William D. , Klempner Mark S. , Schiffer Celia A. , Wang Yang 
Published: November 2016

AbstractThe murine monoclonal antibody LA‐2 recognizes a clinically protective epitope on outer surface protein (OspA) of Borrelia burgdorferi, the causative agent of Lyme disease in North America. Human antibody equivalence to LA‐2 is the best serologic correlate of protective antibody responses following OspA vaccination. Understanding the structural and functional basis of the LA‐2 protective epitope is important for developing OspA‐based vaccines and discovering prophylactic antibodies against Lyme disease. Here, we present a detailed structure‐based analysis of the LA‐2/OspA interaction interface and identification of residues mediating antibody recognition. Mutations were introduced into both OspA and LA‐2 on the basis of computational predictions on the crystal structure of the complex and experimentally tested for in vitro binding and borreliacidal activity. We find that Y32 and H49 on the LA‐2 light chain, N52 on the LA‐2 heavy chain and residues A208, N228 and N251 on OspA were the key constituents of OspA/LA‐2 interface. These results reveal specific residues that may be exploited to modulate recognition of the protective epitope of OspA and have implications for developing prophylactic passive antibodies.
Intravoxel incoherent motion diffusion‐weighted MRI during chemoradiation therapy to characterize and monitor treatment response in human papillomavirus head and neck squamous cell carcinoma
Authors: Paudyal Ramesh , Oh Jung Hun , Riaz Nadeem , Venigalla Praveen , Li Jingao , Hatzoglou Vaios , Leeman Jonathan , Nunez David Aramburu , Lu Yonggang , Deasy Joseph O. , Lee Nancy , Shukla‐Dave Amita 
Published: November 2016

PurposeCharacterize and monitor treatment response in human papillomavirus (HPV) head and neck squamous cell carcinoma (HNSCC) using intra‐treatment (intra‐TX) imaging metrics derived from intravoxel incoherent motion (IVIM) diffusion‐weighted magnetic resonance imaging (DW‐MRI).Materials and MethodsThirty‐four (30 HPV positive [+] and 4 HPV negative [‐]) HNSCC patients underwent a total of 136 MRI including multi‐b value DW‐MRI (pretreatment [pre‐TX] and intra‐TX weeks 1, 2, and 3) at 3.0 Tesla. All patients were treated with chemo‐radiation therapy. Monoexponential (yielding apparent diffusion coefficient [ADC]) and bi‐exponential (yielding perfusion fraction [f], diffusion [D], and pseudo‐diffusion [D*] coefficients) fits were performed on a region of interest and voxel‐by‐voxel basis, on metastatic neck nodes. Response was assessed using RECISTv1.1. The relative percentage change in D, f, and D* between the pre‐ and intra‐TX weeks were used for hierarchical clustering. A Wilcoxon rank‐sum test was performed to assess the difference in metrics within and between the complete response (CR) and non‐CR groups.ResultsThe delta (Δ) change in volume (V)1wk‐0wk for the CR group differed significantly (P = 0.016) from the non‐CR group, while not for V2wk‐0wk and V3wk‐0wk (P > 0.05). The mean increase in ΔD3wk‐0wk for the CR group was significantly higher (P = 0.017) than the non‐CR group. ADC and D showed an increasing trend at each intra‐TX week when compared with pre‐TX in CR group (P < 0.003). Hierarchical clustering demonstrated the existence of clusters in HPV + patients.ConclusionAfter appropriate validation in a larger population, these IVIM imaging metrics may be useful for individualized treatment in HNSCC patients.Level of Evidence: 2J. Magn. Reson. Imaging 2017;45:1013–1023
Content of cadmium and lead in raw, fried and baked commercial frozen fishery products consumed in Poland
Authors: Winiarska‐Mieczan Anna , Grela Eugeniusz R 
Published: November 2016

AbstractBACKGROUNDThe study aimed to verify whether the consumption of frozen fishery products was safe in terms of Cd and Pb content. The study material was 31 frozen fishery products (15 breaded products and 16 fillets). Immediately before the analyses the products were subject to culinary treatment according to the recommendations of the producer: fried in colza oil or baked in a gas oven. The level of Cd and Pb was determined using the GF AAS method.RESULTSThe analysed frozen products contained on average 14.0 µg Cd kg−1 and 18.5 µg Pb kg−1. Compared to raw products, baked fish contained 56% more Cd and 72% more Pb, whereas fried fish contained 16% more Cd and 15% more Pb. Compared to fried products, baked fish contained 34% more Cd and 49% more Pb.CONCLUSIONThe content of Cd and Pb in the products did not exceed the acceptable standard. However, it cannot be clearly stated which method of culinary treatment of frozen fishery products is the best with regard to the level of Cd and Pb in the final products. © 2016 Society of Chemical Industry
Evaluation of polyphenolic content and antioxidant activity in two onion varieties grown under organic and conventional production systems
Authors: Ren Feiyue , Reilly Kim , Gaffney Michael , Kerry Joseph P , Hossain Mohammad , Rai Dilip K 
Published: November 2016

AbstractBACKGROUNDOnions contain a number of bioactive compounds, in particular polyphenols. They are rich sources of such compounds in the human diet and offer significant health benefits to the consumer. Demand for organic crops is steadily increasing partly based on the expected health benefits of organic food consumption. The current study examines the influence of organic and conventional crop management practices on bioactive polyphenolic content of onion.RESULTSWe examined the effect of conventional, organic, and mixed cultivation practices on the content of total phenolics, total flavonoids and antioxidant activity in two varieties of onion grown over 4 years in a split‐plot factorial systems comparison trial. Levels of total phenolics and total flavonoids showed a significant year‐on‐year variation and were significantly different between organic and conventional production systems. The levels of total phenolics, total flavonoids and antioxidant activity in general were significantly higher (P < 0.05) under fully organic compared to fully conventional management.CONCLUSIONOrganic cultivation practices resulted in significantly higher levels of potential bioactive compounds in onion. © 2016 Society of Chemical Industry
Recurrent laryngeal nerve regeneration using an oriented collagen scaffold containing Schwann cells
Authors: Chitose Shun‐ichi , Sato Kiminori , Fukahori Mioko , Sueyoshi Shintaro , Kurita Takashi , Umeno Hirohito 
Published: November 2016

Objectives/HypothesisRegeneration of the recurrent laryngeal nerve (RLN), which innervates the intrinsic laryngeal muscles such that they can perform complex functions, is particularly difficult to achieve. Synkinesis after RLN neogenesis leads to uncoordinated movement of laryngeal muscles. Recently, some basic research studies have used cultured Schwann cells (SCs) to repair peripheral nerve injuries. This study aimed to regenerate the RLN using an oriented collagen scaffold containing cultured SCs.Study DesignPreliminary animal experiment.MethodsA 10‐mm‐long autologous canine cervical ansa was harvested. The nerve tissue was scattered and subcultured on oriented collagen sheets in reduced serum medium. After verifying that the smaller cultivated cells with high nucleus‐cytoplasm ratios were SCs, collagen sheets with longitudinally oriented cells were rolled and inserted into a 20‐mm collagen conduit. The fabricated scaffolds containing SCs were autotransplanted to a 20‐mm deficient RLN, and vocal fold movements and histological characteristics were observed.ResultsScaffolds containing cultured SCs were successfully fabricated. Immunocytochemical examination revealed that these isolated and cultured cells, identified as SCs, expressed S‐100 protein and GFAP but not vimentin. The orientation of SCs matched that of the oriented collagen sheet. Two months after successful transplantation, laryngeal endoscopy revealed coordinated movement of the bilateral vocal folds by external stimulation under light general anesthesia. Hematoxylin and eosin staining showed that the regenerated RLN lacked epineurium surrounding the nerve fibers and was interspersed with collagen fibers. Myelin protein zero was expressed around many axons.ConclusionsPartial regeneration of RLN was achieved through the use of oriented collagen scaffolding.Level of EvidenceNA Laryngoscope, 127:1622–1627, 2017
Changes in electromyographic amplitudes but not latencies occur with endotracheal tube malpositioning during intraoperative monitoring for thyroid surgery: Implications for guidelines
Authors: Barber Samuel R. , Liddy Whitney , Kyriazidis Natalia , Cinquepalmi Matteo , Lin Brian M. , Modi Rahul , Patricio Stephanie , Kamani Dipti , Belotti Carlo , Mahamad Sadhana , Lawson Bradley , Randolph Gregory W. 
Published: November 2016

Objectives/HypothesisDuring intraoperative neural monitoring (IONM) in thyroid and parathyroid surgery, endotracheal (ET) tube migration can result in a decrease in vocalis electromyographic (EMG) amplitude without a concordant latency elevation during stimulation of the recurrent laryngeal nerve (RLN).Study DesignRetrospective review.MethodsData were reviewed retrospectively for thyroid and parathyroid surgery patients with IONM of the laryngeal nerves from January 2015 to December 2015. Recordings of vocalis EMG amplitudes and latencies with RLN stimulation were obtained with the neuromonitoring ET tube surface electrodes in optimal baseline position, with vertical displacement away from the vocalis, and with rotational change away from baseline.ResultsET tube surface electrode EMG recordings were obtained with stimulation of seven left and three right RLNs in a total of 10 patients. Mean vocalis EMG amplitudes were reduced with vertical displacement 1 and 2 cm both inferior and superior to baseline and with rotational change (45° and 90° clockwise and counterclockwise, 180°), although amplitude change with 45° clockwise and 180° rotation did not meet statistical significance. Mean EMG latency values did not change significantly from baseline with either rotation or vertical displacement of the ET tube.ConclusionsAn isolated decrease in EMG amplitude without concordant latency elevation should warrant re‐evaluation of ET tube position during thyroid and parathyroid surgery and is in contrast to a combined event, with both EMG amplitude decrease and concordant latency increase, which is more suggestive of a true neuropraxic injury.Level of Evidence4. Laryngoscope, 127:2182–2188, 2017
Scaffold‐free cartilage tissue engineering with a small population of human nasoseptal chondrocytes
Authors: Chiu Loraine L.Y. , To William T.H. , Lee John M. , Waldman Stephen D. 
Published: November 2016

ObjectiveCartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demonstrate the application of the bioreactor to generate large‐sized tissues from a small population of primary human nasoseptal chondrocytes.Study DesignExperimental study.MethodsChondrocytes were cultured in the bioreactor using different medium compositions, with varying amounts of serum and with or without growth factors. Resulting engineered tissues were analyzed for physical properties, biochemical composition, tissue microstructure, and protein localization.ResultsBioreactor‐cultivated constructs grown with serum and growth factors (basic fibroblast growth factor and transforming growth factor beta 2) had greater thickness, as well as DNA and glycosaminoglycan (GAG) contents, compared to low serum and no growth factor controls. These constructs also showed the most intense proteoglycan and collagen II staining.ConclusionThe combination of bioreactor conditions, serum, and growth factors allowed the generation of large, thick scaffold‐free human cartilaginous tissues that resembled the native nasoseptal cartilage. There also may be implications for patient selection in future clinical applications of these engineered tissues because their GAG content decreased with donor age.Level of EvidenceNA. Laryngoscope, 127:E91–E99, 2017
Electrophysiological identification of nonrecurrent laryngeal nerves
Authors: Kandil Emad , Anwar Muhammad A. , Bamford Jeremy , Aslam Rizwan , Randolph Gregory W. 
Published: November 2016

Objectives/HypothesisThe nonrecurrent laryngeal nerve (NRLN) is a common anatomic variation of the right recurrent laryngeal nerve (RLN), which has been associated with an increased risk of injury during thyroid and parathyroid surgery. We suggest that early successful identification using intraoperative nerve monitoring (IONM) and preservation of this variant will help the surgeon to avoid injury to this nerve. Our objective was to examine the electrophysiological parameters of the NRLN and the efficacy of IONM for successful identification of the NRLN.Study DesignRetrospective database analysis.MethodsThis is a retrospective study of a prospectively collected database of all patients who underwent thyroid and parathyroid surgeries by a single surgeon at a tertiary care center over 3 years (n = 481). Patients' demographic data and operative reports, including the IONM reports, were reviewed. Average stimulation thresholds with resulting amplitudes and latencies were compared. Preoperative and postoperative laryngoscopy were performed in all cases.ResultsWe identified 15 NRLNs (2.2%) in a total 682 laryngeal nerves. No left‐sided NRLNs were observed. The average right vagus latency in the NRLN group was shorter than that of the RLN group (2.40 ms ± 0.49 ms vs. 3.43 ms ± 1.03 ms; P < .001). No statistically significant difference was observed between the initial amplitudes of the right vagus nerves in the two groups (713.67 μV ± 208.71 μV vs. 816.22 μV ± 470.45 μV; P = .14). All NRLN cases exhibited normal functioning of vocal cords on postoperative laryngoscopy.ConclusionsIONM is highly effective in the identification of the NRLN. Right vagus nerve latency in the NRLN group was found to be significantly shorter than in the RLN group. Early identification of the NRLN allowed cautious preservation of the NRLN, resulting in excellent postoperative outcomes. The rate of NRLN identification may be improved by routine use of IONM.Level of Evidence4 Laryngoscope, 127:2189–2193, 2017
Accuracy of endoscopic and videofluoroscopic evaluations of swallowing for oropharyngeal dysphagia
Authors: Giraldo‐Cadavid Luis Fernando , Leal‐Leaño Lorena Renata , Leon‐Basantes Guillermo Alfredo , Bastidas Alirio Rodrigo , Garcia Rafael , Ovalle Sergio , Abondano‐Garavito Jorge E. 
Published: November 2016

ObjectivesA systematic review and meta‐analysis of the literature was conducted to compare the accuracy with which flexible endoscopic evaluation of swallowing (FEES) and videofluoroscopic swallowing study (VFSS) assessed oropharyngeal dysphagia in adults.Data SourcesPubMed, Embase, and the Latin American and Caribbean Health Sciences Literature (LILACS) database.MethodsA review of published studies was conducted in parallel by two groups of researchers. We evaluated the methodological quality, homogeneity, threshold effect, and publication bias. The results are presented as originally published, then with each test compared against the other as a reference and both compared against a composite reference standard, and then pooled using a random effects model. Software use consisted of Meta‐DiSc and SPSS.ResultsThe search yielded 5,697 articles. Fifty‐two articles were reviewed in full text, and six articles were included in the meta‐analysis. FEES showed greater sensitivity than VFSS for aspiration (0.88 vs. 0.77; P = .03), penetration (0.97 vs. 0.83; P = .0002), and laryngopharyngeal residues (0.97 vs. 0.80; P < .0001). Sensitivity to detect pharyngeal premature spillage was similar for both tests (VFSS: 0.80; FEES: 0.69; P = .28). The specificities of both tests were similar (range, 0.93–0.98). In the sensitivity analysis there were statistically significant differences between the tests regarding residues but only marginally significant differences regarding aspiration and penetration.ConclusionsFEES had a slight advantage over VFSS to detect aspiration, penetration, and residues. Prospective studies comparing both tests against an appropriate reference standard are needed to define which test has greater accuracy.Level of Evidence2a Laryngoscope, 127:2002–2010, 2017
Evaluation of peripheral nerve regeneration through biomaterial conduits via micro‐CT imaging
Authors: Pixley Sarah K. , Hopkins Tracy M. , Little Kevin J. , Hom David B. 
Published: November 2016

ObjectiveHollow nerve conduits made of natural or synthetic biomaterials are used clinically to aid regeneration of peripheral nerves damaged by trauma or disease. To support healing, conduit lumen patency must be maintained until recovery occurs. New methods to study conduit structural integrity would provide an important means to optimize conduits in preclinical studies. We explored a novel combined technique to examine structural integrity of two types of nerve conduits after in vivo healing.Study DesignMicro‐CT imaging with iodine contrast was combined with histological analysis to examine two different nerve conduits after in vivo nerve reconstruction in rats.Materials and MethodsSciatic nerve gaps in adult Lewis rats were reconstructed with poly(caprolactone) (PCL, 1.6 cm gap, 14‐week survival) or silicone (1 cm gap, 6‐week survival) conduits (N = 12 total). Conduits with regenerating tissues were imaged by micro‐CT with iodine contrast and compared to the histology (hematoxylin and eosin, immunostaining for axons) of regenerated tissues after iodine removal.ResultsPCL nerve conduits showed extensive breakage throughout their length, but all showed successful nerve growth through the conduits. The silicone conduits remained intact, although significant constriction was uniquely detected by micro‐CT, with 1 of 6 animals showing incomplete tissue regeneration.ConclusionsMicro‐CT with iodine contrast offers a unique and valuable means to determine 3D structural integrity of nerve conduits and nerve healing following reconstruction. Furthermore, this paper shows that even if conduit compression and degradation occur, nerve regeneration can still take place.
The Role of Qualitative Research in Current Digital Social Media: Issues and Aspects—An Introduction
Authors: Gonçalves Helena Martins , Rey‐Martí Andrea , Roig‐Tierno Norat , Miles Morgan P. 
Published: November 2016

ABSTRACTThis paper introduces current research that focuses on the role of qualitative research in social media including the new ways of marketing promotion, participation, and interaction enabled by social media. The articles included in this special issue of Psychology & Marketing were selected from papers submitted to the 6th Global Innovation and Knowledge Academy (GIKA) conference held in March 2016 in Valencia, Spain. This paper provides a brief synthesis of the contributions of these articles to understanding social media's impact on the ability of organizations to better engage with and market to customers.
Social Media Marketing: A Literature Review and Implications
Authors: Alves Helena , Fernandes Cristina , Raposo Mário 
Published: November 2016

ABSTRACTThis study carries out content analysis and systemizes articles on social media marketing in the Web of Science database. Forty‐four studies were analyzed in accordance with a variation on the systematic review approach, involving synthesis‐ and interpretation‐based assessment. The results demonstrate how most of the studies analyzed focus on the consumer perspective in terms of usage, share, and influence of social media on consumer decisions, and perceptions. The studies focusing on the firm's perspective centered not only on the usage of social media, but also on their implementation, optimization, and measurement of results. The majority of studies are quantitative and published in recent years. This study not only reached certain conclusions for both theory and practice, but also defined future lines of research according to the gaps detected by the study's results.
Social Media Marketing For Adolescents
Authors: Mas‐Tur Alicia , Tur‐Porcar Ana , Llorca Anna 
Published: November 2016

ABSTRACTThis article identifies the family traits that characterize adolescents who use social media. Firms exploit information on traits to tailor their strategies to better target their marketing messages. Firms should capitalize on the fact that active communication with consumers improves their relationships with customers. They need to be aware of possible changes in consumers’ behavior patterns to ensure that they are catering to consumers’ interests and demands. This study used qualitative comparative analysis to examine consumers’ use of social media.