bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer
Authors: Zhou Kening , He Yingying , Lin Xi , Zhou Huihao , Xu Xiaomin , Xu Jingui 
Published: September 2024

AbstractEndometrial cancer (EC), as one of the most common cancers, severely threatens female reproductive health. Our previous study has shown that Kinesin family member C1 (KIFC1) played crucial roles in the progression of EC. In addition, abnormal centrosome amplification, which was reported to be partially regulated by KIFC1, usually occurred in different cancers. However, whether KIFC1 promoted EC through centrosome amplification and the potential mechanism remain to be revealed. The present study demonstrated that overexpressed KIFC1, which exhibited a worse prognosis, had a positive correlation with an increased number of centrosomes in human EC samples. In addition, KIFC1 overexpression in EC cells prompted centrosome amplification, chromosomal instability, and cell cycle progression. Moreover, we demonstrated that KIFC1 inhibited E3 ubiquitin-protein ligase TRIM37 to maintain the stability of PLK4 by reducing its ubiquitination degradation, and finally promoting centrosome amplification and EC progression in vitro. Finally, the contributing role of KIFC1 and the inhibitory effect of TRIM37 on EC development and metastasis was verified in a nude mouse xenograft model. Our study elucidated that KIFC1 depends on TRIM37-mediated reduced ubiquitination degradation of PLK4 to promote centrosome amplification and EC progression, thus providing a potential prognostic marker and promising therapeutic target for EC in the future.
Effect of high-dose N-acetylcysteine on exacerbations and lung function in patients with mild-to-moderate COPD: a double-blind, parallel group, multicentre randomised clinical trial
Authors: Zhou Yumin , Wu Fan , Shi Zhe , Cao Jie , Tian Jia , Yao Weimin , Wei Liping , Li Fenglei , Cai Shan , Shen Yao , Wang Zanfeng , Zhang Huilan , Chen Yanfan , Fu Yingyun , He Zhiyi , Chang Chun , Jiang Yongliang , Chen Shujing , Yang Changli , Yu Shuqing , Tian Heshen , Cheng Qijian , Zhao Ziwen , Ying Yinghua , Zhou Yong , Liu Shengming , Deng Zhishan , Huang Peiyu , Zhang Yunzhen , Luo Xiangwen , Zhao Haiyan , Gui Jianping , Lai Weiguang , Hu Guoping , Liu Cong , Su Ling , Liu Zhiguang , Huang Jianhui , Zhao Dongxing , Zhong Nanshan , Ran Pixin ,   On behalf of China N-acetylcysteine in Mild-to-moderate COPD Study Group, Zhao Zhuxiang , Liu Sha , Wang Zihui , Tang Longhui , Zheng Jinzhen , Zhou Hailin , Cai Yuliang , Zhang Yu , Li Haiqing , Chen Ping , Jiang Fen , Cai Suizheng , Zhou Jian , Xia Hong , Wang Meijia , Chu Jiaojiao , Cai Xueding , Li Yazhen , Bin Yanfei , Zeng Lingshan , Zhang Haiqin , He Hua , He Yichen , Zhou Wenxia , Chen Li 
Published: September 2024

AbstractEvidence for the treatment of patients with mild-to-moderate chronic obstructive pulmonary disease (COPD) is limited. The efficacy of N-acetylcysteine (an antioxidant and mucolytic agent) for patients with mild-to-moderate COPD is uncertain. In this multicentre, randomised, double-blind, placebo-controlled trial, we randomly assigned 968 patients with mild-to-moderate COPD to treatment with N-acetylcysteine (600 mg, twice daily) or matched placebo for two years. Eligible participants were 40-80 years of age and had mild-to-moderate COPD (forced expiratory volume in 1 second [FEV1] to forced vital capacity ratio <0.70 and an FEV1 ≥ 50% predicted value after bronchodilator use). The coprimary outcomes were the annual rate of total exacerbations and the between-group difference in the change from baseline to 24 months in FEV1 before bronchodilator use. COPD exacerbation was defined as the appearance or worsening of at least two major symptoms (cough, expectoration, purulent sputum, wheezing, or dyspnoea) persisting for at least 48 hours. Assessment of exacerbations was conducted every three months, and lung function was performed annually after enrolment. The difference between the N-acetylcysteine group and the placebo group in the annual rate of total exacerbation were not significant (0.65 vs. 0.72 per patient-year; relative risk [RR], 0.90; 95% confidence interval [CI], 0.80–1.02; P = 0.10). There was no significant difference in FEV1 before bronchodilator use at 24 months. Long-term treatment with high-dose N-acetylcysteine neither significantly reduced the annual rate of total exacerbations nor improved lung function in patients with mild-to-moderate COPD. Chinese Clinical Trial Registration: ChiCTR-IIR-17012604.
Inverse ceria-nickel catalyst for enhanced C–O bond hydrogenolysis of biomass and polyether
Authors: Zhao Zelun , Gao Guang , Xi Yongjie , Wang Jia , Sun Peng , Liu Qi , Li Chengyang , Huang Zhiwei , Li Fuwei 
Published: September 2024

AbstractRegulating interfacial electronic structure of oxide-metal composite catalyst for the selective transformation of biomass or plastic waste into high-value chemicals through specific C–O bond scission is still challenging due to the presence of multiple reducible bonds and low catalytic activity. Herein, we find that the inverse catalyst of 4CeOx/Ni can efficiently transform various lignocellulose derivatives and polyether into the corresponding value-added hydroxyl-containing chemicals with activity enhancement (up to 36.5-fold increase in rate) compared to the conventional metal/oxide supported catalyst. In situ experiments and theoretical calculations reveal the electron-rich interfacial Ce and Ni species are responsible for the selective adsorption of C–O bond and efficient generation of Hδ− species, respectively, which synergistic facilitate cleavage of C–O bond and subsequent hydrogenation. This work advances the fundamental understanding of interfacial electronic interaction over inverse catalyst and provides a promising catalyst design strategy for efficient transformation of C–O bond.
Development of a novel elastomer with unique properties as fire and radiation resistance
Authors: Mohamed Tarek Mansour , Mahmoud Ghada A. 
Published: September 2024

AbstractThis study aims to create a novel, distinct form of elastomer with superior ability to resist fire, high resistance to radiation, and resistance to environmental conditions such as temperature and solvents. This type of natural-based elastomer was prepared using carboxymethyl cellulose CMC, polyacrylic acid PAA, crosslinked with tannic acid TA. Most techniques in elastomer manufacture technologies are unfriendly and participate in increasing carbon emissions. Gamma radiation was used as a clean tool for copolymerization and crosslinking the elastomer. The irradiation dose of 5 kGy with a rate of 3.32 kGy/h was enough to produce CMC/PAA/TA elastomer. The properties of the produced elastomer were investigated by Fourier-transformed infrared spectroscopy (FT-IR), X-ray diffraction, thermal gravimetric analysis (TGA), and limiting oxygen index (LOI). CMC/PAA/TA has high resistance to solvents such as acetone, benzene, HCl, and HNO3. The tensile strength is 3.376 MPa, the elongation percent is 501.689%, and the LOI value is 30%. The produced elastomer possessed excellent gamma radiation resistance. The elastomer was exposed later to 1864 kGy of gamma radiation without showing degradation and retained its properties, as confirmed by FTIR, TGA, and mechanical properties. After investigation, it can be inferred that the produced CMC/PAA/TA elastomer exhibited outstanding properties.
The effects of threat on complex decision-making: evidence from a virtual environment
Authors: Laycock Aaron , Schofield Guy , McCall Cade 
Published: September 2024

AbstractIndividuals living and working in dangerous settings (e.g., first responders and military personnel) make complex decisions amidst serious threats. However, controlled studies on decision-making under threat are limited given obvious ethical concerns. Here, we embed a complex decision-making task within a threatening, immersive virtual environment. Based on the Iowa Gambling Task (IGT), a paradigm widely used to study complex decision-making, the task requires participants to make a series of choices to escape a collapsing building. In Study 1 we demonstrate that, as with the traditional IGT, participants learn to make advantageous decisions over time and that their behavioural data can be described by reinforcement-learning based computational models. In Study 2 we created threatening and neutral versions of the environment. In the threat condition, participants performed worse, taking longer to improve from baseline and scoring lower through the final trials. Computational modelling further revealed that participants in the threat condition were more responsive to short term rewards and less likely to perseverate on a given choice. These findings suggest that when threat is integral to decision-making, individuals make more erratic choices and focus on short term gains. They furthermore demonstrate the utility of virtual environments for making threat integral to cognitive tasks.
Design and dynamic emulation of hybrid solar-wind-wave energy converter (SWWEC) for efficient power generation
Authors: Jariwala Aryan Manan , Dash Santanu Kumar , Sahu Umesh Kumar , Chudjuarjeen Saichol 
Published: September 2024

AbstractAs research into wave energy converters progresses and new developers enter the field, there arises a growing requirement for a standardized modelling approach. This article presents a novel design and dynamic emulation for a hybrid solar-wind-wave energy converter (SWWEC) which is the combination of three very well-known renewable energies: solar, wind and wave energy. Photovoltaic (PV) panels and vertical axis wind turbine (VAWT) are installed on top of the floating WEC that harness the energies from the sun and wind respectively. The SWWEC is designed with a point absorber capture system. An electrical motor is used to dynamically emulate the performance of the SWWEC under real world conditions to drive the DC generator. The present paper shows the importance and necessity of the required control schemes for the proper control of generator side converters which is present in the offshore marine substation and the most required grid connected onshore converters. The better switching signal generation for the converter control and generated harmonics elimination techniques are also presented in the paper. Outcomes of the present study are discussed and verified.
Uncovering individualised treatment effects for educational trials
Authors: Xiao ZhiMin , Hauser Oliver , Kirkwood Charlie , Li Daniel Z. , Ford Tamsin , Higgins Steve 
Published: September 2024

AbstractLarge-scale Randomised Controlled Trials (RCTs) are widely regarded as “the gold standard” for testing the causal effects of school-based interventions. RCTs typically present the statistical significance of the average treatment effect (ATE), which captures the effect an intervention has had on average for a given population. However, key decisions in child health and education are often about individuals who may be very different from those averages. One way to identify heterogeneous treatment effects across different individuals, not captured by the ATE, is to conduct subgroup analyses. For example, free school meal (FSM) pupils as required for projects funded by the Education Endowment Foundation (EEF) in England. These subgroup analyses, as we demonstrate in 48 EEF-funded RCTs involving over 200,000 students, are usually not standardised across studies and offer flexible degrees of freedom to researchers, potentially leading to mixed, if not misleading, results. Here, we develop and deploy an alternative to ATE and subgroup analysis, a machine-learning and regression-based framework to predict individualised treatment effects (ITEs). ITEs could show where an intervention worked, for which individuals, and to what extent. Our findings have implications for decision-makers in fields like education, healthcare, law, and clinical practices concerning children and adolescents.
Multi-year La Niña frequency tied to southward tropical Pacific wind shift
Authors: Wang Guojian , Santoso Agus 
Published: September 2024

AbstractMulti-year La Niña events cause prolonged climate disruptions worldwide, but a systematic understanding of the underlying mechanisms is not yet established. Here we show using observations and models from the sixth phase of Coupled Model Intercomparison Project that a greater frequency of consecutive La Niña events is tied to the upper equatorial Pacific Ocean when it favors more rapid heat discharge. The propensity for heat discharge is underscored by negative skewness in upper-ocean heat content, underpinned by southward tropical Pacific wind shift during austral summer. Models with stronger westerly anomalies south of the equator simulate steeper east-to-west upward tilt of the thermocline that is favorable for a greater discharge rate. This highlights the crucial role of the southward wind shift in the nonlinear system of the El Niño-Southern Oscillation. The large inter-model spread in multi-year La Niña processes underscores the need in constraining models for reliable climate prediction and projection.
Satellite observations indicate that chia uses less water than other crops in warm climates
Authors: Kirsch Brian , Fisher Joshua B. , Piechota Thomas , Hassani Mohammad , Suardiaz Diego C. , Puri Radhika , Cahill Joseph , Atamian Hagop S. 
Published: September 2024

AbstractMany parts of the world face severe and prolonged drought conditions, stressing the sustainability of water resources and agriculture. Transitioning to water-efficient crops is one strategy that can help adapt to water scarcity. An emerging drought-tolerant crop of interest is chia (Salvia hispanica). Yet, no study has compared its large-scale water use dynamics to those of widely established crops across the globe. Here, we use satellite data over multiple years to assess the water use efficiency of chia, alfalfa, corn, and soybean globally. Results show that chia consumed 13-38% less water than alfalfa, corn, and soy and assimilated 14-20% more carbon per amount of water used. Substituting 10% of Southwest United States alfalfa cultivation with chia would save 184 million liters of water per growing season, equivalent to the annual water consumption of 1,300 households. Future research shall explore the economic, societal, and environmental ramifications of substituting alfalfa with chia in dry areas worldwide. These insights can guide decision-makers in promoting sustainable agriculture and water resource management.
Poor compliance with germline testing recommendations in colorectal cancer patients undergoing molecular residual disease testing
Authors: Schrock-Kelley Suzanne , Souter Vivienne , Hall Michael J. , Sha Youbao , Sengupta Urmi , ElNaggar Adam C. , Liu Minetta C. , Weitzel Jeffrey N. 
Published: September 2024

Abstract
Background
Approximately 15% of colorectal cancers (CRCs) are associated with germline mutations. There is increasing adoption of DNA-based assays for molecular residual disease (MRD) and growing evidence supporting its clinical utility, particularly for CRC by oncologists in the U.S. We assessed the uptake of germline multi-gene panel testing (MGPT) for hereditary cancer in CRC patients receiving MRD analyses in community oncology settings.

Methods
This retrospective study included 80 patients receiving care for CRC through community oncology practices who were referred for MRD testing at a commercial laboratory (January–March 2022). Clinical data, including test requisition forms, pathology reports, and clinical notes were reviewed. Documentation of tumor microsatellite instability and/or immunohistochemical (IHC) testing for mismatch repair (MMR) deficiency, age of CRC diagnosis, family history of cancer, and any order or recommendation for MGPT were assessed.

Results
Overall, 5/80 (6.3%) patients in the study have documented germline MGPT; 65/80 (81.3%) patients have documented MMR testing of their colorectal tumor. Among the 5 cases with abnormal MMR IHC, 2 have MGPT. Of the 33 patients meeting the 2021 National Comprehensive Cancer Network (NCCN) criteria for genetic/familial high-risk assessment, only 2 have MGPT.

Conclusions
Our real-world data suggest that many CRC patients receiving MRD testing and meeting NCCN (v. 2021) criteria for germline MGPT may not be receiving evaluation beyond routine MMR status. Process and educational improvements are needed in community health settings to increase access and uptake of germline testing among CRC patients regardless of age at diagnosis or MMR status.
A review of marine genetic resource valuations
Authors: Krusberg Tilde , Schildt Lova , Jouffray Jean-Baptiste , Zhivkoplias Erik , Blasiak Robert 
Published: September 2024

AbstractMarine biotechnology is a rapidly growing sector of the ocean economy, but efforts to assess its value, and that of the genetic resources that enable it, remain contentious. Through a comprehensive literature review, we identified 67 distinct valuations of marine genetic resources. We analysed these using the IPBES Values Assessment typology and found a predominance of behaviour-based valuations using economic value indicators to assess monetary benefits from marine drug discovery.
Transition metal homoeostasis is key to metabolism and drug tolerance of Mycobacterium abscessus
Authors: Liu Yi , Murphy Katy , Fernandes Nadia , Moore Rebekah E. T. , Pennisi Ivana , Williams Richard , Rehkämper Mark , Larrouy-Maumus Gerald 
Published: September 2024

AbstractAntimicrobial resistance (AMR) is one of the major challenges humans are facing this century. Understanding the mechanisms behind the rise of AMR is therefore crucial to tackling this global threat. The presence of transition metals is one of the growth-limiting factors for both environmental and pathogenic bacteria, and the mechanisms that bacteria use to adapt to and survive under transition metal toxicity resemble those correlated with the rise of AMR. A deeper understanding of transition metal toxicity and its potential as an antimicrobial agent will expand our knowledge of AMR and assist the development of therapeutic strategies. In this study, we investigate the antimicrobial effect of two transition metal ions, namely cobalt (Co2+) and nickel (Ni2+), on the non-tuberculous environmental mycobacterium and the opportunistic human pathogen Mycobacterium abscessus. The minimum inhibitory concentrations of Co2+ and Ni2+ on M. abscessus were first quantified and their impact on the bacterial intracellular metallome was investigated. A multi-omics strategy that combines transcriptomics, bioenergetics, metabolomics, and phenotypic assays was designed to further investigate the mechanisms behind the effects of transition metals. We show that transition metals induced growth defect and changes in transcriptome and carbon metabolism in M. abscessus, while the induction of the glyoxylate shunt and the WhiB7 regulon in response to metal stresses could be the key response that led to higher AMR levels. Meanwhile, transition metal treatment alters the bacterial response to clinically relevant antibiotics and enhances the uptake of clarithromycin into bacterial cells, leading to increased efficacy. This work provides insights into the tolerance mechanisms of M. abscessus to transition metal toxicity and demonstrates the possibility of using transition metals to adjuvant the efficacy of currently using antimicrobials against M. abscessus infections.
First detection of Jingmen tick virus in Corsica with a new generic RTqPCR system
Authors: Cicculli Vincent , Colmant Agathe M. G. , Piorkowski Géraldine , Amaral Rayane , Maitre Apolline , Decarreaux Dorine , Thirion Laurence , Moureau Gregory , Falchi Alessandra , de Lamballerie Xavier , Charrel Remi N. , Ayhan Nazli 
Published: September 2024

AbstractJingmen tick virus (JMTV) is a recently discovered segmented RNA virus, closely related to flaviviruses. It was identified for the first time in 2014, in China and subsequently in Brazil. Following this discovery, JMTV-related sequences have been identified in arthropods, vertebrates (including humans), plants, fungus, and environmental samples from Asia, America, Africa, Europe, and Oceania. Several studies suggest an association between these segmented flavi-like viruses, termed jingmenviruses, and febrile illness in humans. The development of rapid diagnostic assays for these viruses is therefore crucial to be prepared for a potential epidemic, for the early detection of these viruses via vector surveillance or hospital diagnosis. In this study, we designed a RT-qPCR assay to detect tick-associated jingmenviruses, validated it and tested its range and limit of detection with six tick-associated jingmenviruses using in vitro transcripts. Then, we screened ticks collected in Corsica (France) from different livestock species, in order to determine the distribution of these viruses on the island. In total, 6269 ticks from eight species were collected from 763 cattle, 538 horses, 106 sheep, and 218 wild boars and grouped in 1715 pools. We report the first detection of JMTV in Corsica, in Rhipicephalus bursa, Hyalomma marginatum and R. sanguineus ticks collected from cattle and sheep. The highest prevalence was found in the Rhipicephalus genus. The complete genome of a Corsican JMTV was obtained from a pool of Rhipicephalus bursa ticks and shares between 94.7% and 95.1% nucleotide identity with a JMTV sequence corresponding to a human patient in Kosovo and groups phylogenetically with European JMTV strains. These results show that a Mediterranean island such as Corsica could act as a sentinel zone for future epidemics.
Aspartyl proteases target host actin nucleator complex protein to limit epithelial innate immunity
Authors: Patra Sandip , Kaur Rupinder 
Published: September 2024

AbstractEpithelial-immune cell communication is pivotal to control microbial infections. We show that glycosylphosphatidylinositol-linked aspartyl proteases (Yapsins) of the human opportunistic pathogenic yeast Candida glabrata (Cg) thwart epithelial cell (EC)-neutrophil signalling by targeting the EC protein, Arpc1B (actin nucleator Arp2/3 complex subunit), which leads to actin disassembly and impeded IL-8 secretion by ECs. Further, the diminished IL-8 secretion inhibits neutrophil migration, and protects Cg from the neutrophil-mediated killing. CgYapsin-dependent Arpc1B degradation requires Arginine-142 in Arpc1B, and leads to reduced Arpc1B-p38 MAPK interaction and downregulated p38 signalling. Consistently, Arpc1B or p38 deletion promotes survival of the Cg aspartyl protease-deficient mutant in ECs. Importantly, kidneys of the protease-deficient mutant-infected mice display elevated immune cell infiltration and cytokine secretion, implicating CgYapsins in immune response suppression in vivo. Besides delineating Cg-EC interplay, our results uncover a novel target, Arpc1B, that pathogens attack to constrain the host signalling networks, and link Arpc1B mechanistically with p38 activation.