bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alternative Oxidase Transcription Factors AOD2 and AOD5 ofNeurospora crassaControl the Expression of Genes Involved in Energy Production and Metabolism
Authors: Qi Zhigang , Smith Kristina M , Bredeweg Erin L , Bosnjak Natasa , Freitag Michael , Nargang Frank E 
Published: February 2017

AbstractIn Neurospora crassa, blocking the function of the standard mitochondrial electron transport chain results in the induction of an alternative oxidase (AOX). AOX transfers electrons directly from ubiquinol to molecular oxygen. AOX serves as a model of retrograde regulation since it is encoded by a nuclear gene that is regulated in response to signals from mitochondria. The N. crassa transcription factors AOD2 and AOD5 are necessary for the expression of the AOX gene. To gain insight into the mechanism by which these factors function, and to determine if they have roles in the expression of additional genes in N. crassa, we constructed strains expressing only tagged versions of the proteins. Cell fractionation experiments showed that both proteins are localized to the nucleus under both AOX inducing and noninducing conditions. Furthermore, chromatin immunoprecipitation and high throughput sequencing (ChIP-seq) analysis revealed that the proteins are bound to the promoter region of the AOX gene under both conditions. ChIP-seq also showed that the transcription factors bind to the upstream regions of a number of genes that are involved in energy production and metabolism. Dependence on AOD2 and AOD5 for the expression of several of these genes was verified by quantitative PCR. The majority of ChIP-seq peaks observed were enriched for both AOD2 and AOD5. However, we also observed occasional sites where one factor appeared to bind preferentially. The most striking of these was a conserved sequence that bound large amounts of AOD2 but little AOD5. This sequence was found within a 310 bp repeat unit that occurs at several locations in the genome.
Variants of the Sir4 Coiled-Coil Domain Improve Binding to Sir3 for Heterochromatin Formation in Saccharomyces cerevisiae
Authors: Samel Anke , Rudner Adam , Ehrenhofer-Murray Ann E 
Published: February 2017

Abstract
Heterochromatin formation in the yeast Saccharomyces cerevisiae is characterized by the assembly of the Silent Information Regulator (SIR) complex, which consists of the histone deacetylase Sir2 and the structural components Sir3 and Sir4, and binds to unmodified nucleosomes to provide gene silencing. Sir3 contains an AAA+ ATPase-like domain, and mutations in an exposed loop on the surface of this domain abrogate Sir3 silencing function in vivo, as well in vitro binding to the Sir2/Sir4 subcomplex. Here, we found that the removal of a single methyl group in the C-terminal coiled-coil domain (mutation T1314S) of Sir4 was sufficient to restore silencing at the silent mating-type loci HMR and HML to a Sir3 version with a mutation in this loop. Restoration of telomeric silencing required further mutations of Sir4 (E1310V and K1325R). Significantly, these mutations in Sir4 restored in vitro complex formation between Sir3 and the Sir4 coiled-coil, indicating that the improved affinity between Sir3 and Sir4 is responsible for the restoration of silencing. Altogether, these observations highlight remarkable properties of selected amino-acid changes at the Sir3-Sir4 interface that modulate the affinity of the two proteins.
Rheumatoid arthritises biobankkal szerzett tapasztalataink: 204 beteg biológiai mintáinak és klinikai adatainak összevetése
Authors: Pál Ildikó , Pusztai Anita , Csomor Péter , Szekanecz Zoltán 
Published: February 2017

Abstract: Introduction: A biobank is a registry, which is suitable for the storage of biological samples (e.g. tissues, DNA, protein), genetical abnormalities and clinical data. Several biobanks have been created worldwide, which contribute to research and the better understanding of disease pathogenesis, genetical polymorphisms. Biobanking also helps to improve the efficacy of therapies. Aim: Our purpose was to create an internet-based biobank, in which laboratory test results, genetic alterations and related disorders of rheumatoid arthritis (RA) patients can be registered. This biobank would be able to make the research easier and it can help to improve our knowledge about diseases and it can inhibit loss of data. Patients and method: We have biological samples from 204 RA patients and we have entered their data in the biobank which can be found on the website http://rheuma.biobank.eu . Statistical analysis was performed by SPSS20 statistical programme. Results: By the creation of biobank that contains clinical data and biological samples of 204 RA patients, we have a database which can help to improve our knowledge about the disease and help to develop new treatment strategies. Conclusion: Biobanking is suitable to analyze blood samples and clinical data together. Orv. Hetil., 2017, 158(7), 270–277.
DINAMIKA KONDISI OSEANOGRAFI MUSIMAN PERAIRAN SELAT SUNDA DARI ANALISIS DATA MULTITEMPORAL
Authors: Amri Khairul , Manurung Djisman , Siregar Vincentius P. 
Published: February 2017

Tujuan riset ini adalah untuk mengkaji kondisi oseanografi musiman (sebaran suhu permukaan laut, konsentrasi klorofil-a, pola arus, dan salinitas) perairan Selat Sunda secara musiman. Kajian ini menggunakan data hasil pengukuran in situ dan data penginderaan jauh multitemporal tahun 2000, 2001, 2002, dan 2004. Analisis dilakukan secara visual dan digital untuk mendapatkan gambaran dinamik mengenai kondisi oseanografi musiman perairan Selat Sunda. Hasil yang diperoleh menunjukkan bahwa nilai sebaran suhu permukaan laut Selat Sunda sepanjang tahun bervariasi, di mana nilai berkisar antara 27,0 sampai dengan 30,5°C, lebih tinggi dibanding dengan nilai sebaran hasil pengukuran in situ. Salinitas berkisar antara 31,0 sampai dengan 33,7‰ dengan nilai terendah (31,0‰) pada musim barat, sementara salinitas tertinggi (32,7 sampai dengan 33,7‰) ditemukan pada musim peralihan 2. Sebaran klorofil-a berkisar antara 0,1 sampai dengan 2,0 mg m-3. Musim barat merupakan musim dengan kandungan klorofil-a terendah 0,1 mg m-3 dan musim timur merupakan musim dengan tingkat kesuburan perairan tertinggi (1,5 sampai dengan 2,0 mg m-3). Diduga peningkatan produktivitas primer yang sangat tinggi pada musim timur selain akibat aliran massa air yang kaya nutrien dari Laut Jawa, juga akibat dari proses upwelling pada mulut selat bagian selatan. The aim of this research is to study the dynamic of the seasonal oceanography condition (sea surface temperature, chlorophyll-a concentration, sea surface height anomaly, and salinity), of the Sunda Straits waters using in situ data and satellite multitemporal images until 2000, 2001, 2002, and 2004. The oceanographic data were analyzed using visual and digital analyze to find the dynamic features. Results show that sea surface temperature was fluctuated with seasons. The values ranging from 27.0 to 30.5°C were higher than in situ measurement. The Surface salinity varied with the value of 31.0 to 33.7‰. The Lowest salinity (31.0‰) was found of the west monsoon, the highest salinity (33.7‰) at the inter monsoon 2. The Concentration of chlorophyll-a (0.1 to 2.0 mg m-3) with the highest abundance at east monsoon. The high est concentration of chlorophyll a in east monsoon may be occurred by impact of nutrient transport from Java Sea and also by contribution of upwelling process in southern mouth of Sunda Strait.
ASPEK BIOLOGI IKAN KENYAR Sarda orientalis FAMILI: SCOMBRIDAE DARI PERAIRAN SAMUDERA HINDIA
Authors: Pralampita Wiwiet An , Mardlijah Siti , Chodriyah Umi 
Published: February 2017

Ikan kenyar (Sarda orientalis), merupakan komoditi perikanan yang tergolong ekonomis penting, selain sebagai komoditi ekspor juga untuk memenuhi permintaan pasar domestik, namun data dan informasi mengenai ikan tersebut sangat kurang, baik data statistik, maupun data biologi serta potensi dan distribusi kelimpahan. Penelitian tentang aspek biologi kenyar hasil tangkapan dari perairan Samudera Hindia telah dilakukan. Selama ini penelitian khusus mengenai ikan kenyar secara mendalam belum pernah dilakukan di Indonesia. Menurut hasil penghitungan nisbah kelamin, perbandingan kelamin ikan kenyar jantan dan betina berada dalam keadaan tidak seimbang, dan didapatkan betina lebih banyak. Kisaran ukuran panjang (FL) ikan kenyar dari Samudera Hindia 17,0 sampai dengan 69,0 cm. Hubungan panjang bobot ikan kenyar bersifat isometrik, dengan demikian pertambahan panjang ikan kenyar, seimbang dengan pertambahan bobot. Komposisi kenyar betina selama bulan pengamatan didominasi oleh kenyar dengan tingkat kematangan gonad III dan IV, yaitu lebih dari 50% jumlah, hampir di setiap bulan pengamatan. Striped bonito (Sarda orientalis) is fishery commodity which has important economic value. However, there is still lack of data and information about the fish, not only in statistic data but also biology data, potent, and distribution data. Research on biology aspect of striped bonito Sarda orientalis from Indian Ocean waters was conducted. Intensif research in striped bonito (Sarda orientalis) has not been done in Indonesia. Based on calculation of sex rate, ratio of male and female kenyar is very unbalance where the female is much more then the male. The length rate of striped bonito which is landed in Binuangeun, Pelabuhan Ratu, Cilacap, and Kedonganan vary from 17.0 until 69.0 cm. The length weight corelation is isometric, indicate the more length the fish get, the more weight the fish get. The composition of in female striped bonito during the observation is dominated by the fish with gonate mature III and IV, which is more than 50% from all the fish that observed.
Lexical Features of Editorial Comments of Tell Magazine in Nigeria
Authors: Ariyo Kayode Samuel 
Published: February 2017

The Editorial Comment of news print is highly significant for the negotiation of meaning. This is made possible through linguistic features that characterise the comments, one of which is the lexical cohesive ties that provide a distinct linguistic connection in the text. The analysis of selected editorial comments is based on Halliday’s and Hasan’s (1976) Lexical Cohesion Theory. The study therefore investigates the social functions of the observed lexical devices in the editorial comments in order to demonstrate how meanings are being foregrounded in the editorial comments of the news print.
Strengthening and Repair of Parabolic RC Beam Using Carbon Fiber – Reinforced Polymer Under Repeated Load
Authors: Sulaivani Bayar , Amin Tara 
Published: February 2017

The aim of this study is to evaluate the effect of (CFRP) on parabolic beams under repeated load. In this study nine parabolic reinforced concrete beams were prepared having (150 x 200mm) dimensions , two unstrengthened as control beams and others were divided in to two groups (A and B) .
Group (A) consisted of four beams wrapped with (CFRP) in different ways , group (B) included three damaged beams loaded to about (55%) of the maximum load of control beams and then repaired by (CFRP) . All beams tested under repeated load by (10kN) in one cycle.
The test results showed the (CFRP) increased the strength of the parabolic RC beams based on the shape and type of the strengthening.
Transient Tooth Discoloration After Periodontal Instrumentation of Aggressive Periodontitis: A Case Report
Authors: Rincon Julio C. , Oakley Zahida , Abbott Paul 
Published: February 2017

Introduction: Transient tooth discoloration (TTD) is an endodontic condition associated with dental traumatic events. To the best of the authors’ knowledge, this is the first case report of transient coronal discoloration of the two maxillary permanent central incisors after periodontal instrumentation of root surfaces affected by aggressive periodontitis (AgP). The initial case presentation and multidisciplinary periodontal, endodontic, and orthodontic management are described.Case Presentation: A 17‐year‐old male was referred from the orthodontics department to the periodontics department of the Oral Health Center of Western Australia, Perth, Western Australia, Australia. Localized AgP was diagnosed. After meticulous periodontal debridement of the mid‐root and apical root portions of the two maxillary central incisors, TTD was noted 5 months later. The diagnosis, case management, and biologic explanations for the unique presentation of these conditions are discussed.Conclusions: TTD may be caused by trauma from periodontal instrumentation. Proper diagnosis and management of this particular condition is important to protect the integrity of the pulp in affected teeth. This case report opens the possibility of TTD originating from causes other than traumatic luxation or intra‐alveolar root fractures of teeth.
Growth and molting of mud crab administered by different doses of vitomolt
Authors: Fujaya Yushinta 
Published: February 2017

<p>Vitomolt is a molting stimulant made from spinach (<em>Amaranthus</em> spp.) extracts. The objective of this study was to optimize the dose of vitomolt injection on the growth and molting of mud crab (<em>Scylla </em>spp). The research was conducted in April to June 2010. There were three doses of vitomolt tested, i.e. 9 mg/g crab, 15 mg/g, and 21 mg/g crab. The results showed that the dose of vitomolt injection had great influence on the growth and molting of mud crab. Higher dose of vitomolt gave higher growth but its molting percentage was different. Vitomolt dose of 15 mg/g crab was the optimal dose to induce molting of mud crab while the dose of 21 mg/g crab gave the highest growth which reached 53.6%. However, in terms of productivity, dose of 15 mg/g crab gave the highest production of soft crab.</p> <p>Key words: dose, vitomolt, growth, molting, mud crab</p> <p> </p> <p>ABSTRAK</p> <p><em>Vitomolt</em> adalah stimulan <em>molting</em> yang terbuat dari ekstrak bayam (<em>Amaranthus</em> spp.). Tujuan penelitian ini adalah optimalisasi dosis penyuntikan <em>vitomolt</em> terhadap pertumbuhan dan <em>molting</em> kepiting bakau (<em>Scylla </em>spp). Penelitian dilakukan pada bulan April hingga Juni 2010. Ada tiga dosis <em>vitomolt</em> yang diuji, yakni: 9 mg/g, 15 mg/g, dan 21 mg/g kepiting. Hasilnya menunjukkan bahwa penyuntikan <em>vitomolt</em> sangat berpengaruh terhadap pertumbuhan dan <em>molting</em> kepiting bakau. Semakin tinggi dosis <em>vitomolt</em> memberikan pertumbuhan yang semakin tinggi pula namun tidak demikian terhadap persentase <em>molting</em>. Dosis <em>vitomolt</em> 15 mg/g kepiting adalah dosis optimal menginduksi <em>molting</em> kepiting bakau, sedangkan dosis 21 mg/g kepiting memberikan pertumbuhan tertinggi hingga 53,6%. Bila ditinjau dari produktivitas, dosis 15 mg/g kepiting memberikan produksi kepiting lunak tertinggi.</p> <p>Kata kunci: dosis, <em>vitomolt</em>, pertumbuhan, <em>molting</em>, kepiting bakau</p>
Profiles of traditional farms: soil texture, total inorganic N and bacteria-producing estate
Authors: Hastuti Yuni Puji , Rusmana Iman , Widiyanto T. 
Published: February 2017

<p>Pond traditional system is the pond in still activity with a symple management system. This activity indicated by low technology and relatively low production level. Aquaculture activities in traditional pond not loss from nitrification and denitrification prosess, however this process is more low production rather than semiintensive and intensive system. This study aims to observe abundance of bacteria nitrification along with changes soil texture, and N-organic in the soil of traditional pond. Chemical and biological analyses were done using spectroscopy and Most Probable Number methods to determine the amount of nitrite and ammonium production of bacteria. Based of the result, each stratum traditional ponds have relatively similar abundance in nitrite producing bacteria of 7.08-7.47 Log CFU/g. Increasing abundance in ammonium producing bacteria was found in all stratum, range from 5.63 Log cfu/g to 8.12 Log cfu/g. From the first day of preparation, traditional ponds have a lot of nitrite and ammonium producing bacteria.<br /><br />Keywords: traditional, pond, nitrification, abundance of bacteri.</p><p> </p><p><strong>ABSTRAK</strong><br /><br />Tambak sistem tradisional merupakan tambak yang dalam kegiatannya masih menggunakan sistem manajemen sederhana. Hal ini ditandai dengan penerapan teknologi sederhana, dan tingkat produksi relatif rendah. Kegiatan budidaya di tambak tradisional tidak akan terlepas dari proses nitrifikasi dan denitrifikasi, namun demikian proses ini relatif lebih rendah aktivitasnya daripada tambak sistem semiintensif dan intensif. Tujuan dari penelitian ini adalah mempelajari kelimpahan bakteri penghasil senyawa nitrit, amonium seiring dengan perubahan tekstur tanah, dan N-organik pada tanah tambak tradisional. Media pertumbuhan bakteri dikondisikan bebas oksigen (oxygen free nitrogen/OFN method) , sedangkan kelimpahan bakteri dianalisis dengan rumus most porbable number (MPN). Berdasarkan hasil, setiap strata tanah tambak tradisional memiliki jumlah bakteri penghasil nitrit yang relatif sama, yaitu antara 7,08-7,47 Log cfu/g. Peningkatan kelimpahan bakteri penghasil amonium terjadi pada semua strata, yaitu berkisar antara 5,63 Log cfu/g sampai dengan 8,12 Log cfu/g. Dari hari pertama persiapan, tambak tradisional telah memiliki kelimpahan bakteri penghasil nitrit dan amonium yang berlimpah.<br /><br />Kata kunci: tradisional, tambak, nitritifikasi, kelimpahan bakteri.<br /></p>
Study of gonadotropin (GtH) stimulating hormone on gonad maturation of climbing perch Anabas testudineus Bloch
Authors: Suriansyah . , Junior M. Zairin , Sudrajat Agus Oman 
Published: February 2017

<p>Gonadotropin can quicken the process of 17α-hidroxyprogesterone hormone synthesis becoming 17α, 20β-di hidroxyprogesterone as the maturation inducing steroids (MIS) and quicken the process of egg nucleus integration to germinal vesicle breakdown (GVBD) position. This research aimed to know the efficacy of gonadotropin hormone in the form of ovaprim on gonad maturation of climbing perch (<em>Anabas testudineus</em> Bloch). Stimulation of GtH with a dose of 0.5 ml/kg of fish body weight could improve the fish gonado somato index (GSI) to 2.72 %, improve the the final egg diameter to 0.70 mm (71.50 %), and shorten ovulation time which down to 4.30 hours.</p> <p>Key words: Gonadotropin hormone, gonad maturation, <em>Anabas testudineus</em></p> <p><em> </em></p> <p>ABSTRAK</p> <p>Hormon gonadotropin dapat mempercepat proses sintesa hormon 17α-hidroksiprogesteron menjadi 17α, 20β-dihidroksiprogesteron yang berfungsi sebagai steroid yang merangsang pematangan gonad dan mempercepat proses integrasi inti sel telur menuju posisi <em>germinal vesicle breakdowan</em> (GVBD). Penelitian ini bertujuan untuk mengetahui efektivitas hormon gonadotropin yang terdapat dalam ovaprim terhadap pematangan gonad ikan betook <em>Anabas testudineus</em> Bloch. Pemberian hormon GtH dalam bentuk ovaprim dengan dosis 0,5 ml/kg bobot ikan dapat memperbaiki perkembangan gonad yang ditunjukkan dengan peningkatan nilai gonado somatik indeks (GSI) sebanyak 2,72%, peningkatan diameter telur menjadi 0,77 mm (71,50%) dan mempercepat waktu ovulasi menjadi 4,3 jam.</p> <p>Kata-kata kunci: Hormon gonadotropin, pematangan gonad, ikan betok</p>
The Effect of Problem-Based Learning and Level Intelligence of Students’ Critical Thinking on Kalamm Science
Authors: Astuti Mardiah 
Published: February 2017

This research aim was to figure out the effect of PBL and Level Intelligence of Student’s Critical Thinking. The subject matter in this research was Ilmu Kalam. This research was experimental design and used factorial design 2 x 2. The result of this research was: 1) There were differences in average critical thinking skills in the subject of Kalam Science of the group given, the model PBM to Conventional Methods, 2) there was evidence that the PBL model could improve critical thinking skills for students who had high intelligence, 3) The ability of students in critical thinking in that they had low intelligence by using higher PBM model with critical thinking skills of students with lower intelligence levels using conventional models, 4) the interaction effect between PBL and intelligence influential on the level of critical thinking skills of students.Tujuan penelitian ini adalah untuk mengetahui pengaruh PBL dan Tingkat Kecerdasan Berpikir Kritis Mahasiswa. Subyek dalam penelitian ini adalah Ilmu Kalam. Penelitian ini adalah desain eksperimental dan digunakan desain faktorial 2 x 2. Hasil penelitian ini adalah: 1) Ada perbedaan keterampilan berpikir kritis rata-rata di subjek Kalam Ilmu kelompok tertentu, model PBM ke Metode Konvensional, 2) ada bukti bahwa model PBL dapat meningkatkan kemampuan berpikir kritis bagi siswa yang memiliki kecerdasan yang tinggi, 3) kemampuan siswa dalam berpikir kritis dalam bahwa mereka memiliki kecerdasan yang rendah dengan menggunakan model PBM lebih tinggi dengan kemampuan berpikir kritis siswa dengan tingkat kecerdasan yang lebih rendah menggunakan model konvensional, 4) efek interaksi antara PBL dan kecerdasan berpengaruh pada tingkat kemampuan berpikir kritis siswa.
Analisis Kesulitan Belajar Mahasiswa Dalam Memahami Konsep Bilangan Real
Authors: Sucipto Lalu , Mauliddin Mauliddin 
Published: February 2017

[Bahasa]: Penelitian ini bertujuan untuk mengetahui jenis-jenis kesulitan dan faktor-faktor penyebab kesulitan yang dihadapi oleh mahasiswa dalam mengerjakan soal bilangan real. Penelitian ini merupakan penelitian kualitatif dengan menggunakan analisis deskriptif. Data dikumpulkan melalui observasi, tes dan wawancara. Hasil tes dikelompokkan berdasarkan tujuh kategori kesalahan Watson kemudian dilakukan wawancara untuk mengklarifikasi kesalahan tersebut.Hasil penelitian ini menunjukkan bahwa jenis kesalahan yang dominan terjadi pada jawaban mahasiswa berupa jenis kesalahan data tidak tepat, prosedur yang tidak tepat, konflik level respon, dan masalah hirarkhi keterampilan. Kesalahan mahasiswa dalam mengerjakan soal memberikan gambaran kesulitan yang dialami dalam memahami konsep bilangan real. Faktor yang mempengaruhi kesulitan mahasiswa yaitu: materi yang sulit dipahami dan diaplikasikan dalam pemecahan masalah; dan faktor pribadi yang mencakup pola belajar yang tidak baik, sarana pendukung, dan faktor dosen.
[English]: This research aims to identify undergraduate students’ difficulties and the factors which caused them in solving real analysis problems. This qualitative research applies descriptive analysis. Data is collected through observation, test, and interview. Students’ answers on test are categorized into Watson’s seven categories of errors then the students are interviewed to clarify the errors. This research reveals that dominant errors encounterd by the students are inappropriate data, inappropriate procedure, response level conclict, and skill hierarchy problem. The errors portrait the difficulties the students encountered. The factors which cause the difficulties are the material is hard to understand and apply to problem solving; and personal factor such as bad learning habits, supporting tools, and the lecturers.