bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Somatization Rates, Frequent Spontaneous Recovery, and a Lack of Organic Biomarkers in Post‐Covid‐19 Condition
Authors: Tröscher Anna , Gebetsroither Patrick , Rindler Marc , Böhm Vincent , Dormann Rainer , von Oertzen Tim , Heidbreder Anna , Helbok Raimund , Wagner Judith 
Published: October 2024

ABSTRACTIntroductionMany patients report neuropsychiatric symptoms after SARS‐CoV‐2 infection. Data on prevalence of post‐COVID‐19 condition (PCC) vary due to the lack of specific diagnostic criteria, the report of unspecific symptoms, and reliable biomarkers.MethodsPCC patients seen in a neurological outpatient department were followed for up to 18 months. Neurological examination, SARS‐CoV‐2 antibodies, Epstein–Barr virus antibodies, and cortisol levels as possible biomarkers, questionnaires to evaluate neuropsychiatric symptoms and somatization (Patient Health Questionnaires D [PHQ‐D]), cognition deficits (Montreal Cognitive Assessment [MoCA]), sleep disorders (ISS, Epworth Sleepiness Scale [ESS]), and fatigue (FSS) were included.ResultsA total of 175 consecutive patients (78% females, median age 42 years) were seen between May 2021 and February 2023. Fatigue, subjective stress intolerance, and subjective cognitive deficits were the most common symptoms. Specific scores were positive for fatigue, insomnia, and sleepiness and were present in 95%, 62.1%, and 44.0%, respectively. Cognitive deficits were found in 2.3%. Signs of somatization were identified in 61%, who also had an average of two symptoms more than patients without somatization. Overall, 28% had a psychiatric disorder, including depression and anxiety. At the second visit (n = 92), fatigue (67.3%) and insomnia (45.5%) had decreased. At visit three (n = 43), symptom load had decreased in 76.8%; overall, 51.2% of patients were symptom‐free. Biomarker testing did not confirm an anti‐EBV response. SARS‐CoV‐2‐specific immune reactions increased over time, and cortisol levels were within the physiological range.ConclusionDespite high initial symptom load, 76.8% improved over time. The prevalence of somatization and psychiatric disorders was high. Our data do not confirm the role of previously suggested biomarkers in PCC patients.
Evaluating the Effects of Different Cognitive Tasks on Autonomic Nervous System Responses: Implementation of a High‐Precision, Low‐Cost Complementary Method
Authors: Ahmadi Nazli Karimi , Ozgur Sezgi Firat , Kiziltan Erhan 
Published: October 2024

ABSTRACTIntroductionWe developed a low‐cost, user‐friendly complementary research tool to evaluate autonomic nervous system (ANS) activity at varying levels of cognitive workload. This was achieved using visual stimuli as cognitive tasks, administered through a specially designed computer‐based test battery.MethodsTo assess sympathetic stress responses, skin conductance response (SCR) was measured, and electrocardiograms (ECG) were recorded to evaluate heart rate variability (HRV), an indicator of cardiac vagal tone. Twenty‐five healthy adults participated in the study. SCR and ECG recordings were made during both tonic and phasic phases using a computer‐based system designed for visual stimuli. Participants performed a button‐pressing task upon seeing the target stimulus, and the relationship between reaction time (RT) and cognitive load was evaluated.ResultsAnalysis of the data showed higher skin conductance levels (SCLs) during tasks compared to baseline, indicating successful elicitation of sympathetic responses. RTs differed significantly between simple and cognitive tasks, increasing with mental load. Additionally, significant changes in vagally mediated HRV parameters during tasks compared to baseline highlighted the impact of cognitive load on the parasympathetic branch of the ANS, thereby influencing the brain–heart connection.ConclusionOur findings indicate that the developed research tool can successfully induce cognitive load, significantly affecting SCL, RTs, and HRV. This validates the tool's effectiveness in evaluating ANS responses to cognitive tasks.
Navigating disturbances in developing countries: Resilience strategies for cross‐sector partnerships
Authors: Oukes Tamara , Beninger Stefanie , Derks Milou , Nijhof André 
Published: October 2024

AbstractThe world faces significant challenges, particularly in low‐income countries, where cross‐sector partnerships strive to create positive social change. Operating under severe uncertainty, these partnerships encounter various disturbances threatening their progress. Despite these challenges, our study explores the resilience strategies that these partnerships use to foster positive social change, an area previously underexplored. Our study uses a qualitative multiple‐case study of four cross‐sector partnerships in sub‐Saharan Africa. Specifically, we collected data via interviews, workshops, and archival sources and used thematic analysis to uncover key resilience strategies. Our findings reveal a cyclical process where, when facing disturbances, cross‐sector partnerships respond with resilience strategies that help them mitigate disruptions, adapt to changing conditions, and continue expanding their positive impact. In doing so, they drive positive social change through core activities within their socio‐ecological system. This research expands existing theories of organizational resilience by highlighting how cross‐sector partnerships in low‐income contexts can not only survive, but also expand their impact of positive social change, through resilience.
ISO 14001 and corporate financial performance: A Systematic Literature Review
Authors: Benzidia Smaïl , Rahoui Siham , Ouiakoub Mohamed , Rostan Louise 
Published: October 2024

AbstractThe impact of the certification on financial performance is still a debated issue. While a number of research studies have highlighted the importance of the environmental management system in achieving environmental performance, there is a theoretical gap concerning the way in which the environmental management system affects the financial performance of companies. This paper aims to fill the identified gap on how ISO 14001 adoption affects companies' financial performance, and the moderating factors likely to influence this link. To address the research question, a systematic literature review was carried out over the last decade of the main studies on this issue published in peer‐reviewed journals between 2004 and 2024. The analysis of the 57 articles in our sample provides a comprehensive picture of the impact of ISO 14001 on financial performance. The results of this research highlight the factors that influence the relationship between financial performance and ISO 14001 certification and suggest that endogenous and exogenous factors should be taken into account in order to understand this complex link.
Symptom Network Analysis and Unsupervised Clustering of Oncology Patients Identifies Drivers of Symptom Burden and Patient Subgroups With Distinct Symptom Patterns
Authors: Bergsneider Brandon H. , Armstrong Terri S. , Conley Yvette P. , Cooper Bruce , Hammer Marilyn , Levine Jon D. , Paul Steven , Miaskowski Christine , Celiku Orieta 
Published: October 2024

ABSTRACTBackgroundInterindividual variability in oncology patients' symptom experiences poses significant challenges in prioritizing symptoms for targeted intervention(s). In this study, computational approaches were used to unbiasedly characterize the heterogeneity of the symptom experience of oncology patients to elucidate symptom patterns and drivers of symptom burden.MethodsSeverity ratings for 32 symptoms on the Memorial Symptom Assessment Scale from 3088 oncology patients were analyzed. Gaussian Graphical Model symptom networks were constructed for the entire cohort and patient subgroups identified through unsupervised clustering of symptom co‐severity patterns. Network characteristics were analyzed and compared using permutation‐based statistical tests. Differences in demographic and clinical characteristics between subgroups were assessed using multinomial logistic regression.ResultsNetwork analysis of the entire cohort revealed three symptom clusters: constitutional, gastrointestinal‐epithelial, and psychological. Lack of energy was identified as central to the network which suggests that it plays a pivotal role in patients' overall symptom experience. Unsupervised clustering of patients based on shared symptom co‐severity patterns identified six patient subgroups with distinct symptom patterns and demographic and clinical characteristics. The centrality of individual symptoms across the subgroup networks differed which suggests that different symptoms need to be prioritized for treatment within each subgroup. Age, treatment status, and performance status were the strongest determinants of subgroup membership.ConclusionsComputational approaches that combine unbiased stratification of patients and in‐depth modeling of symptom relationships can capture the heterogeneity in patients' symptom experiences. When validated, the core symptoms for each of the subgroups and the associated clinical determinants may inform precision‐based symptom management.
N‐Acetyl‐L‐Cysteine (NAC) Blunts Axitinib‐Related Adverse Effects in Preclinical Models of Glioblastoma
Authors: Formato Alessia , Salbini Maria , Orecchini Elisa , Pellegrini Manuela , Buccarelli Mariachiara , Vitiani Lucia Ricci , Giannetti Stefano , Pallini Roberto , D'Alessandris Quintino Giorgio , Lauretti Liverana , Martini Maurizio , De Falco Valentina , Levi Andrea , Falchetti Maria Laura , Mongiardi Maria Patrizia 
Published: October 2024

ABSTRACTObjectiveAxitinib is a tyrosine kinase inhibitor characterized by a strong affinity for Vascular Endothelial Growth Factor Receptors (VEGFRs). It was approved in 2012 by Food and Drug Administration and European Medicines Agency as a second line treatment for advanced renal cell carcinoma and is currently under evaluation in clinical trial for the treatment of other cancers. Glioblastoma IDH‐wild type (GBM) is a highly malignant brain tumor characterized by diffusely infiltrative growth pattern and by a prominent neo‐angiogenesis. In GBM, axitinib has demonstrated a limited effectiveness as a monotherapy, while it was recently shown to significantly improve its efficacy in combination treatments. In preclinical models, axitinib has been reported to trigger cellular senescence both in tumor as well as in normal cells, through a mechanism involving intracellular reactive oxygen species (ROS) accumulation and activation of Ataxia Telangiectasia Mutated kinase (ATM). Limiting axitinib‐dependent ROS increase by antioxidants prevents senescence specifically in normal cells, without affecting tumor cells.MethodsWe used brain tumor xenografts obtained by engrafting Glioma Stem Cells (GSCs) into the brain of immunocompromised mice, to investigate the hypothesis that the antioxidant molecule N‐Acetyl‐L‐Cysteine (NAC) might be used to reduce senescence‐associated adverse effects of axitinib treatment without altering its anti‐tumor activity.ResultsWe demonstrate that the use of the antioxidant molecule N‐Acetyl‐Cysteine (NAC) in combination with axitinib stabilizes tumor microvessels in GBM tumor orthotopic xenografts, eventually resulting in vessel normalization, and protects liver vasculature from axitinib‐dependent toxicity.ConclusionOverall, we found that NAC co‐treatment allows vessel normalization in brain tumor vessels and exerts a protective effect on liver vasculature, therefore minimizing axitinib‐dependent toxicity.
Renal Protection of HWL‐088and ZLY‐032, Two Dual GPR40/PPARδ Agonists, in Adenine‐Induced Renal Fibrosis Model
Authors: Geng Xinqian , Huang Wanqiu , Deng Liming , Xiong Yuxin , Zhao Yunli , Yao Huixin , Zhou Zongtao , Xu Bo , Xu Fan , Wang Feiying , Wang Xiaoling , Li Yiping , Tao Wenyu , Li Zheng , Yang Ying 
Published: October 2024

AbstractThis research examined the potential of novel GPR40/PPARδ dual agonists, HWL‐088 and ZLY‐032, to protect the kidneys in a mouse model of adenine‐induced renal fibrosis. Mice were given a diet containing 0.25 % adenine to develop renal fibrosis and then received different dosages of HWL‐088 or ZLY‐032. After being euthanized, tissue and serum samples were collected for morphological, histological, and molecular examination. Compared to the control group, mice fed adenine showed an increase in kidney‐to‐body weight ratio, serum creatinine, and urea levels. Hematoxylin and eosin staining revealed alleviated glomerulosclerosis, tubular dilation, and inflammatory cell infiltration in mice treated with HWL‐088 or ZLY‐032. Furthermore, Masson staining and immunohistochemistry demonstrated that these dual agonists protected against renal interstitial fibrosis and inflammation, corroborated by decreased expression levels of fibrosis‐related proteins (TGF‐β, Collα1, TIMP‐1) and pro‐inflammatory cytokines (TNF‐α, IL‐1β, IL‐6). Accordingly, it can be inferred that GPR40/PPARδ dual agonists HWL‐088 and ZLY‐032 could yield significant renoprotective effects by inhibiting inflammation and fibrosis. Overall, these results may contribute to the development of novel therapeutic strategies for renal fibrosis.
Biodiversity and Bioprospecting of Fungal Endophytes from Houttuynia cordata Thunb. as a Potential Antibacterial and Anticancer Agent
Authors: Anshory Tamhid Hady , Sukmarini Linda , Fitria Annisa , Davi Setiawan Iwang , Wahyu Wardana Andika , Ilyas Muhammad , Pramisandi Amila , Triastuti Asih 
Published: October 2024

AbstractEndophytic fungi are considered a new source of bioactive compounds that have important applications in agriculture and medicine. This study aims to investigate the biodiversity and potential of endophytic fungi isolated from Houttuynia cordata Thunb. as antimicrobials and anticancer agents. Out of ten isolated endophytes, four species have never been reported to be associated with H. cordata: Ceratobasidium sp., Cladosporium sp., Phomopsis sp., and Fusarium sp. The antibacterial activity assay revealed that the ethyl acetate extract of Ceratobasidium sp. HCS‐3 possessed most potent antibacterial activity against Escherichia coli and Staphylococcus aureus. In addition, its cytotoxic activity test showed the promising anticancer activity on lung cancer A549, osteosarcoma MG‐63, and cervical cancer HeLa cells with IC50 of 4.55±1.16, 32.14±2.78, and 1.54±0.66 ppm, respectively. Furthermore, metabolite profiling identified 66 compounds suggesting that benzoic acid, farnesol, and cyclopeptides may contribute to the antibacterial activity, while 4‐methoxycinnamic acid may have anticancer potential.
Antifungal Activity of Novel Isoindoline‐2‐Yl Putrescines as Potential Autophagy‐Activated Fungicide
Authors: Zhu Lisong , Huang Fengcheng , Wang Hongye , Zhao Yanjun , Luan Shaorong , Xiao Ciying , Huang Qingchun 
Published: October 2024

AbstractThe exacerbation of plant fungal diseases necessitates the development of new fungicides to prevent outbreaks. In this study, five novel isoindoline‐2‐yl putrescines (ISPs) were synthesized, and their synthetic procedures and gram‐scale preparation were explored. When tested at 50 μg mL−1, ISPs did not significantly inhibit mycelial growth on agar plates. However, at 100 μg mL−1, they demonstrated remarkable in vivo efficacy in mitigating Botrytis cinerea infection, especially ISP3 showed curative and protective activities of 91.9 % and 92.6 %, respectively. Moreover, ISP3 also effectively halted lesion expansion of gray mold, Sclerotic rot, and Fusarium scabs, while inducing excessive malformed top mycelial branches of B. cinerea and Sclerotinia sclerotiorum, suppressing sclerotia formation in S. sclerotiorum, and triggering autophagic vacuolization with numerous autophagosomes in the mycelia of these fungi. Molecular docking revealed that ISP3 effectively bound to the active site of BcAtg3, forming hydrogen bonds with Ser279, Gly343, Asp370, and Asp13, along with establishing a stable salt bridge with Asp13. Furthermore, ISP3 possessed favorable ADMET properties. These findings highlight ISP3 as a promising antifungal candidate through autophagy activation.
Three New Spirostan Glycosides from Dracaena Cochinchinensis with NO Production Inhibitory and Antimicrobial Activity
Authors: Hai Yen Pham , Thi Hai Yen Duong , Huu Tai Bui , Huy Hoang Nguyen , Thi Thanh Huong Phan , Anh Bang Ngo , Thi Dung Duong , Thi Trang Do , Anh Le Tuan , Van Kiem Phan 
Published: October 2024

AbstractThree new spirostan glycosides, dracochinosides A–C (1–3), and four known steroidal glycosides (4–7) were isolated from the aerial parts of Dracaena cochinchinensis (Lour.) S.C.Chen. Their chemical structures were determined by the IR, HR‐ESI‐MS, 1D‐, and 2D‐NMR spectra. Compounds 1–3, 6 and 7 inhibited nitric oxide production in LPS activated RAW 264.7 cells with IC50 values ranging from 57.5 to 92.8 μM. In addition, all the isolated compounds exhibited at least one of seven tested microbial strains with the MIC values ranging from 0.016 to 0.128 mg/mL. This is the first report of compounds 5–7 from the genus Dracaena.
Convenient Partial Reduction of CO2 to a Useful C1 Building Block: Efficient Access to 13C‐Labelled N‐Heterocyclic Carbenes
Authors: Phillips Nicholas A. , Sapsford Joshua S. , Csókás Dániel , Kótai Bianka , Perez‐Tabarnero Ines , Díez‐González Silvia , Scott Daniel J. , Pápai Imre , Ashley Andrew E. 
Published: October 2024

AbstractThe selective, transition metal‐free hydrosilylation of CO2 to CH2(OSiEt3)2 has been achieved under mild conditions and in high isolated yields (up to 90%) by using Et3SiH and the simple, easily prepared borohydride catalyst Li+[HB(C6F5)3]−. The resulting CO2‐derived bis(silyl)acetal product—whose mechanism of formation has been interrogated through detailed computational and experimental studies—can be rapidly valorized through the facile synthesis of N‐heterocyclic carbenes, via their corresponding imidazolium salts. By using relatively inexpensive, isotopically enriched 13CO2 this protocol can be exploited to prepare NHC isotopologues that are selectively 13C labelled at the key, ligating C2 position. This provides an electronically responsive 13C NMR spectroscopic handle with dramatically enhanced sensitivity, which can directly benefit reactivity studies in both organo‐ and organometallic catalysis, where NHC use is ubiquitous.
Persistent Renal Dysfunction After Acute Kidney Injury Among STEMI Patients Undergoing Primary Coronary Intervention: Prevalence and Predictors
Authors: Frydman Shir , Freund Ophir , katash Haytham Abu , Rimbrot Daniel , Banai Shmuel , Shacham Yacov 
Published: October 2024

ABSTRACTBackgroundAcute kidney injury (AKI) is a common and serious complication of ST‐elevation myocardial infarction (STEMI). AKI and chronic kidney disease (CKD) are highly heterogeneous, leaving a wide gap between them. Therefore, the term acute kidney disease (AKD) was implemented, describing prolonged renal injury between 7 and 90 days. We aimed to evaluate the prevalence and predictors of AKD among STEMI patients.MethodsThis retrospective observational study included 2940 consecutive patients admitted with STEMI between 2008 and 2022. Renal function was assessed upon admission and routinely thereafter. Renal outcomes were evaluated according to KDIGO criteria, with AKD defined as persistent renal injury of between 7 and 90 days.ResultsTwo hundred and fifty‐two subjects with STEMI and AKI were included; of them, 117 (46%) developed AKD. Among baseline CKD patients, higher rates of AKD were observed (60% vs. 46%). KDIGO index ≥ 2 was an independent predictor for AKD in in subjects without baseline CKD (AOR 2.63, 95% CI 1.07−6.53). In subjects with baseline CKD, older age and higher creatinine were independent predictors for AKD. Subjects with AKD had a higher 1‐year mortality rate (HR 3.39, 95% CI 1.71−6.72, p < 0.01). This trend was mainly driven by the CKD subpopulation where higher mortality rates for AKD on CKD were observed (HR 5.26, 95% CI 1.83−15.1, p < 0.01).ConclusionAKD is common among STEMI patients with AKI. The presence of CKD and higher KDIGO stage should prompt strict monitoring for early diagnosis, treatment, and prevention of renal function deterioration.
Effect of asymptomatic intervertebral flexion patterns on lumbar disc pressure: A finite element analysis study
Authors: Nematimoez Mehdi , Haddas Ram , Breen Alexander 
Published: October 2024

AbstractMovement patterns may be a factor for manipulating the lumbar load, although little information is yet available in the literature about the relationship between this variable and intervertebral disc pressure (IDP). A finite element model of the lumbar spine (49‐year‐old asymptomatic female) was used to simulate intervertebral movements (L2–L5) of 127 asymptomatic participants. The data from participants that at least completed a simulation of lumbar vertebral movement during the first 53% of a movement cycle (flexion phase) were used for further analyses. Then, for each vertebral angular motion curve with constant spatial peaks, different temporal patterns were simulated in two stages: (1) in lumbar pattern exchange (LPE), each vertebral angle was simulated by the corresponding vertebrae of other participants data; (2) in vertebral pattern exchange (VPE), vertebral angles were simulated by each other. The k‐mean algorithm was used to cluster two groups of variables; peak and cumulative IDP, in both stages of simulations (i.e., LPE and VPE). In the second stage of the simulation (VPE), Kendall's tau was utilized to consider the relationship between different temporal patterns and IDPs for each individual lumbar level. Cluster analyses showed that the temporal movement pattern did not exhibit any effect on the peak IDP while the cumulative IDP changed significantly for some patterns. Earlier involvement in lumbar motion at any level led to higher IDP in the majority of simulations. There is therefore a possibility of manipulating lumbar IDP by changing the temporal pattern with the same ROM, in which optimal distribution of the loads among lumbar levels may be applied as preventive or treatment interventions. Evaluating load benefits, such as load, on biomechanically relevant lumbar levels, dynamically measured by quantitative fluoroscopy, may help inform interventional exercises.