bluuebunny/crossref_metadata_2025_split huggingface dataset:
Debris‐flow fan channel avulsions: An important secondary erosional process along the Ichino‐sawa torrent, Japan
Authors: Tsunetaka Haruka , Hotta Norifumi , Imaizumi Fumitoshi , Hayakawa Yuichi S. 
Published: September 2024

AbstractSediment transported from debris‐flow initiation zones is typically stored in a topographic feature called a debris‐flow fan, the formation process of which governs secondary sediment transport further downstream. Although sediment transport from debris‐flow fans can impact sediment regimes and change landforms, the determinants of progressive fan formation are not well constrained. To identify such determinants, this study monitored debris flows and performed topographic surveys of debris‐flow fans in the Ichino‐sawa torrent (Japan) during 2016–2017. In this period, eight debris flows occurred, two of which eroded the existing fan and formed a new channel with a short recurrence interval (~40 days). Consequently, these two cases induced substantial sediment transport further downstream from the fan. The examined rainfall indices did not provide a threshold for diagnosing the occurrence of such sediment transport. Debris flows with a large flow depth and a long duration led to changes in the runout direction and subsequently formed new channels. Before these processes, the existing channel was backfilled and plugged by previous debris flows, forming a steep fan surface around the fan apex. The results suggest that increasing the magnitude and the duration of debris flows potentially triggers sediment transport from fans coupled with channel plugging. The annual sediment transport from the fan exceeded almost all sediment yields of the world rivers and was found comparable with that linked with volcanic eruptions and their aftermath. Thus, the fan‐formation process can induce substantial sediment transport, independent of volcanic perturbations and extreme climatic events, and is dependent on the sediment supply from repeated occurrence of debris flows in the initiation zones.
Tsunami propagation and flooding maps: An application for the Island of Lampedusa, Sicily Channel, Italy
Authors: Borzì Laura , Scala Pietro , Distefano Salvatore , Laksono F. X. Anjar Tri , Manno Giorgio , Innangi Sara , Gamberi Fabiano , Kovács János , Ciraolo Giuseppe , Di Stefano Agata 
Published: September 2024

AbstractThe Mediterranean coastlines are densely populated zones which host key socio‐economic and commercial activities. For this reason, coastal areas are vulnerable sites in case of natural disasters as tsunamis that can strike coasts causing widespread damage to the population and facilities. For these reasons, several studies were performed over the last decade to study the impact of tsunami waves on the coasts. This research assessed the inundation risk due to a tsunami wave which can hit the southeastern coast of Lampedusa Island. The coastal low‐lying geomorphological setting of the southeastern part of the island led to significant socio‐economic growth, but Lampedusa falls within the Mediterranean Sea, a high‐tsunamigenic area, therefore, the need to investigate tsunami propagation and coastal flooding of this sensitive site emerged. For this scope, a calculation chain model was implemented incorporating three steps: the DELFT‐3D software for earthquake effects modelling, MIKE 21 Flow Model FM for nearshore propagation and HEC‐RAS for onshore tsunami inundation modelling. The simulations illustrate the impact of three tsunami scenarios with different magnitudes (Mw 8.5, 7.5, 6.5) generated by hypothetical earthquakes in the Hellenic Arc. In the Mw 8.5 magnitude scenario, significant flooding occurs in the harbour region, with maximum water depths reaching approximately 3.5 m. The maximum water velocity in this scenario reaches about 15 m/s in the eastern portion, adjacent to cliffs impacted by the tsunami wave. In contrast, the Mw 7.5 magnitude scenario demonstrates reduced flooded areas, with the cliffs containing the waves and preventing further flooding. Water depths and velocities in the Mw 7.5 scenario remain minimal. Changes in both propagation and flooding are not significant between scenarios Mw 7.5 and Mw 6.5. This methodology can be employed for more accurate tsunami wave simulations not only in the Mediterranean region but also in various case studies.
Research of recombinant influenza A virus as a vector for Mycoplasma pneumoniae P1a and P30a
Authors: Yu Liang , Yongbo Wang , Shengjun Yang , Jia Tan , Ya Xu , Guoyang Liao , Linna Ma 
Published: September 2024

AbstractBackgroundMycoplasma pneumoniae (MP) is a common respiratory pathogen affecting the longevity of the elderly and the health of children. However, the human vaccine against MP has not been successfully developed till now due to the poor immunogenicity and side effects of MP inactivated or attenuated vaccine. Therefore, it is necessary to develop a MP genetic engineering vaccine with influenza virus strain as vector.MethodsIn this study, the major antigen genes P1a of MP adhesion factor P1(3862‐4554 bases) and P30a of P30(49‐822 bases) were inserted into the nonstructural protein (NS) gene of Influenza A virus strain A/Puerto Rio/8/34(H1N1), PR8 for short, to construct the recombinant vectors NS‐P1a or NS‐P30a. The recombinant pHW2000 plasmids containing NS‐P1a or NS‐P30a were cotransfected with the rest 7 fragments of PR8 into HEK293T cells. After inoculating chicken embryos, the recombinant influenza viruses rFLU‐P1a and rFLU‐P30a were rescued. RT‐PCR and sequencing were used to identify the recombinant viruses. The hemagglutination titers of rFLU‐P1a and rFLU‐P30a were determined after five successive generations in chicken embryos so as to indicate the genetic stability of the recombinant viruses. The morphology of recombinant influenza viruses was observed under electron microscopy.ResultsP1a or P30a was designed to be inserted into the modified NS gene sequence separately and synthesized successfully. RT‐PCR identification of the recombinant viruses rFLU‐P1a and rFLU‐P30a showed that P1a (693 bp), P30a (774 bp), NS‐P1a (1992bp) and NS‐P30a (2073 bp) bands were found, and the sequencing results were correct. After five successive generations, each virus generation has a certain hemagglutination titer (from 1:32 to 1:64), and the band of P1a or P30a can be seen in the corresponding positions. The virus particles under the electron microscope appeared as spheres or long strips connected by several particles, revealing a complete viral membrane structure composed of virus lipid bilayer, hemagglutinin, neuraminidase, and matrix proteins.ConclusionThe recombinant viruses rFLU‐P1a and rFLU‐P30a which carried the advantaged immune regions of the P1 and P30 genes in MP were successfully constructed and identified. And the genetic stability of rFLU‐P1a or rFLU‐P30a was relatively high. The typical and complete morphology of influenza virus was observed under the electron microscope. Our research provided a foundation for the further development of MP vaccines for human.
What's the story of the elephant? Evaluation of choose‐your‐own‐adventure activities on public perception of human–elephant conflict
Authors: Tan Cedric Kai Wei , Loo Yen Yi , Ahmad Husam Aina Amyrah , Hii Adeline , Wong Ee Phin 
Published: September 2024

AbstractChoose‐your‐own‐adventure (CYOA) narratives offer immersive experiences that can effectively convey complex conservation concepts, fostering empathy and critical thinking, particularly in addressing issues like human–elephant conflict. Despite their potential, there is limited research on the use of CYOA activities in conservation education. In this study, we created an interactive story centered on elephant conservation, drawing from existing research to distill scientific concepts into engaging narratives and utilizing various modes of delivery (YouTube and live performances) to reach diverse audiences. We conducted postactivity surveys to assess variations in audience perception, learning, and conservation engagement intentions in relation to sociodemographic factors, activity type, and messages encountered. We then modeled the relationships between ordinal responses and explanatory variables using cumulative ordinal mixed models (N = 398). For the YouTube version, we also considered sociodemographic factors (YouTube n = 53, non‐YouTube n = 47). Our findings indicate a preference for live performances over online YouTube activity, with participants gaining knowledge about elephant conservation from both formats. For the YouTube activity, participants strongly agreed that the activity allowed engagement with the character and topic. They also expressed a higher likelihood of participating in several conservation actions, relative to a control group. While CYOA storytelling shows promise for conservation education, challenges remain in simplifying scientific language, assessing its impact on comprehension of complex issues, standardizing outcomes, and effectively communicating knowledge. Further research is recommended to adapt this approach, making it applicable to various audiences and domains beyond conservation.
Mitigating the Prozone‐Like Effect in HLA Antibody Testing: A Case Report of Therapeutic Plasma Exchange for Antibody‐Mediated Rejection Post‐Heart Transplantation
Authors: Grewal Sarah K. , Achram Robert , Nakahara Hirotomo , Roback John D. , Morris Anna B. , Gebel Howard M. , Bray Robert A. , Sullivan H. Cliff 
Published: September 2024

ABSTRACTTherapeutic plasma exchange (TPE) is a cornerstone treatment for antibody‐mediated rejection (AMR) post‐organ transplantation, aiming to eliminate pathogenic donor‐specific HLA antibodies (DSA). However, limitations in HLA antibody interpretation due to the prozone‐like effect (PLE) can lead to inaccurate assessment of treatment efficacy. We present a case of a heart transplant recipient with suspected AMR, where an unexpected increase in DSA levels post‐TPE prompted investigation into PLE. Solid‐phase Luminex assays were employed to detect HLA antibodies. Serum was run neat as well as after treatment with ethylenediaminetetraacetic acid (EDTA). Nephelometry was used to detect complement levels. Laboratory analysis of pre‐TPE serum revealed higher DSA levels with EDTA treatment, characteristic of PLE. Complement measurements supported complement‐mediated interference in the pre‐TPE sample. This case underscores the importance of being aware that PLE can occur in HLA testing and can impact the interpretation of TPE efficacy for AMR.
Preemptive Approach to Plerixafor Use Is Optimal in Patients With Relapsed/Refractory Germ Cell Tumors Undergoing Peripheral Blood Hematopoietic Stem Cell Collection: Effect on Collection Days, Yields, and Cost
Authors: Sohutskay David O. , Tetrick Anne M. , Goebel W. Scott , Schwering Dave , Reddy Manasa S. 
Published: September 2024

ABSTRACTEvidence describing the use of plerixafor in the off‐label population of relapsed/refractory germ cell tumors (GCT) is limited. We aim to describe the effect of rescue versus preemptive plerixafor use on apheresis collection days, collection yields, and cost. We retrospectively collected data on 77 consecutive patients (at least 15 years of age) with GCT who underwent peripheral blood stem cell (PBSC) collection for autologous stem cell transplant between January 1, 2020 and May 1, 2022. Depending on insurance approval, plerixafor was given either as “rescue” (after a first apheresis collection of < 5 × 106 CD34+ cells/kg) or as “preemptive” on Day 4 of granulocyte‐colony stimulating factor (G‐CSF) prior to the first apheresis collection, if the Day 4 peripheral blood CD34+ count was < 40 cells/μL. A total of 66% of patients who received preemptive plerixafor completed collection in 1 day, similar to good mobilizers who only needed G‐CSF (71%, p = 0.366). In contrast, all poor mobilizers in the rescue group required at least 2 days of collection and had lower CD34+ cell yields than the preemptive group (7.15 vs. 9.81 × 106/kg, p = 0.0055). A cost analysis revealed that preemptive plerixafor may save approximately $7000 per patient compared with a rescue approach. Preemptive plerixafor in GCT patients undergoing PBSC collection allows relatively poor mobilizers to collect in fewer days and with lower overall cost. Fewer apheresis procedures result in less risk to the patient, increased patient satisfaction, and the ability to schedule more patients within the constraints of staffing.
Efficient acceleration of the convergence of the minimum free energy path via a path‐planning generated initial guess
Authors: Sun Yi 
Published: September 2024

AbstractWe demonstrate that combining a shifted clustering algorithm with a fast‐marching‐based algorithm can generate accurate approximations of the minimum energy path (MEP) given a free energy landscape (FEL). Using this approximation as the initial guess for the MEP, followed by further refinement with the string method (referred to as the fast marching tree (FMT)‐string combined approach), significantly reduces the number of iterations required for MEP convergence. This approach saves substantial time compared to using linear interpolation (LI) for the initial guess. Our method offers a viable solution for obtaining an effective initial guess of the MEP when an approximate or converged FEL is available. This work highlights the potential of applying FMT‐based approaches to extract the MEP in chemical reactions.
Eco‐friendly approaches for synthesis of indolyl 1H‐pyrroles using rice‐husk‐derived carbonaceous sulfonation as the green catalyst
Authors: Nguyen Linh Dieu , Nguyen Khanh Ha , Nguyen Phat Ngoc , Tran Kim Nguyen , Le Diep Dinh , Tran Phuong Hoang , Nguyen Hai Truong 
Published: September 2024

AbstractBACKGROUNDAmorphous carbon‐bearing sulfonic acid groups (AC–SO3H) are a kind of solid acid that exhibits high acidity and belongs to the new generation of solid acids. The presence of acidic functional groups, including carboxylic acid, phenolic and sulfonic acid groups, allows for several key activities such as strong Brønsted acid properties, high surface area, stability, reusability and recyclability.RESULTSIn this work, we synthesized indolyl 1H‐pyrroles from indoles, phenylglyoxal monohydrate, 1,3‐diketones and ammonium acetate using rice‐husk‐derived carbonaceous sulfonation (AC–SO3H) as a catalyst. We explored the impact of catalyst quantity, reaction time, solvent, mineral acid and temperature in order to determine the optimal reaction conditions. Additionally, indolyl 1H‐pyrrole derivatives were also synthesized based on the optimal conditions that were completely investigated, and the structure of these synthetic compounds was characterized by 1H‐ and 13C‐nuclear magnetic resonance.CONCLUSIONThe yield of indolyl 1H‐pyrroles was up to 84% in 6 h at 100 °C with the activity of 30 mg AC–SO3H. Besides, based on the optimal conditions, 21 indolyl 1H‐pyrrole derivatives were formed with the high‐yield and green method. The catalyst could be reused multiple times without a significant decrease in catalytic performance. According to the findings from the experiments, a potential mechanism that included a radical process has been suggested. This eco‐friendly approach provided a straightforward and efficient method to produce a variety of indole–pyrrole conjugates in a single step. © 2024 Society of Chemical Industry (SCI).
Chronic exertional compartment syndrome of the forearm diagnosed in a wider spectrum of patients and not seldom with a history of the same diagnosis in the lower legs
Authors: Sjöcrona Louise , Lindorsson Sophia H. , Rennerfelt Kajsa 
Published: September 2024

AbstractPurposeTo identify patient characteristics associated with forearm chronic exertional compartment syndrome (CECS) and to demonstrate the distribution of intramuscular pressure (IMP) values at 1 min postexercise in a cohort of patients with exercise‐induced forearm pain.MethodsA consecutive series of 99 patients seeking orthopaedic consultation for chronic exertional forearm pain underwent IMP measurements between 2010 and 2023. The diagnosis of CECS was confirmed (n = 34) or ruled out (n = 65) based on the patient's history, clinical examination and IMP measurements.ResultsThere were significantly more male patients in the CECS group than in the group of patients where the diagnosis was ruled out. Furthermore, a significantly higher proportion of the CECS patients had been previously treated for CECS of the lower legs. The most common occupation was office worker (21%), followed by craftsperson (18%). The most common main physical activities were strength training (21%) and cycling (15%). The median (range) 1‐min postexercise IMP values for patients with CECS were 34 (23–68) mmHg for the flexor compartment and 32 (25–67) mmHg for the extensor compartment.ConclusionThis study demonstrates a more general population of CECS patients compared to previous studies. Notably, more than a fifth of the CECS patients had previously been treated for CECS in the lower legs. Importantly, considering the 95% confidence interval for IMP values in patients without CECS, the most used IMP cutoff value for diagnosing CECS appears to be too high for the forearm compartments.Level of EvidenceLevel II.
Oxidative Challenges Do Not Impact Pheomelanin‐Dependent Coloration in Male Japanese Quails
Authors: Alejandro Vianey , Hernández América , Pérez‐Rodríguez Lorenzo , Montoya Bibiana 
Published: September 2024

ABSTRACTColorful traits play an important role in animal communication. Melanin‐based colorations are the most extended color traits in animals and are produced by two types of endogenous melanic pigments: eumelanins and pheomelanins, the last ones being the least studied in the context of communication. The production of pheomelanin requires a semi‐essential amino acid, cysteine, which is also used for the synthesis of an important endogenous antioxidant, glutathione. Hence, it has been proposed that the synthesis of pheomelanin and glutathione may compete for the cysteine available in the organism. In that case, pheomelanic colorations are predicted to be less intense when the individual is facing an oxidative challenge, and therefore, they would provide information on the oxidative status of the bearer. Here, we experimentally evaluated this hypothesis using male Japanese quails (Coturnix japonica) as a model of study, a species with pheomelanin‐based plumage in the breast and cheeks. During feather growth, individuals were exposed to one of three possible conditions: Control (saline), an endogenous oxidative challenge (Escherichia coli lipopolysaccharide injections), or an exogenous oxidative challenge (paraquat injections). Contrary to predictions, we found that: (1) Birds from the three groups exhibited less intense pheomelanic colorations in feathers after the experimental manipulation, and the magnitude of this change did not differ among groups. (2) There was no effect of the experimental treatments on the proportion reduced/oxidized glutathione, an index of oxidative status. (3) Lipid peroxidation was lower after the experimental manipulation, with birds exposed to the paraquat challenge experiencing a stronger decline than other groups. (4) Cysteine and total glutathione levels decreased after the experimental manipulation, with no differences per group in the magnitude of the decline. Taken together the results do not support the hypothesis that oxidative status plays a key role at determining the variation in the intensity of pheomelanic colorations.
The characteristics of general practitioners and geriatricians who take overall responsibility for the care of older patients with multimorbidity
Authors: Kimura Takuma , Matsumura Shinji , Hashimoto Masayoshi , Shinmura Ken 
Published: September 2024

AbstractBackgroundOlder patients with multimorbidity often seek care from multiple health care providers and visit several medical institutions. Having a primary care provider who takes overall responsibility for their care may be beneficial. We conducted a survey to identify the characteristics of general practitioners and geriatricians who frequently assume such responsibility for older patients with multimorbidity.MethodsIn June 2022, we distributed by mail an anonymous questionnaire to 3300 family medicine specialists, primary care‐certified physicians, and geriatric specialists in Japan. We used a four‐point Likert‐type scale to score items related to conditions and patient backgrounds that pose challenges in treatment, key clinical factors, and important clinical strategies. Modified Poisson regression was used to identify factors associated with frequently assuming overall responsibility for medical care.ResultsData from 746 physicians were included in the analysis. Factors associated with frequently assuming overall responsibility for medical care of older patients with multimorbidity included providing inpatient ward care (prevalence ratio [PR] 1.237, 95% CI 1.124–1.362), providing home medical care (PR 1.357, 95% CI 1.225–1.504), frequently treating patients over 90 years old (PR 2.043, 95% CI 1.258–3.318), and the overall score for clinical strategies (PR 1.021, 95% CI 1.010–1.033).ConclusionsGeneral practitioners and geriatricians who frequently assume overall responsibility for the care of older patients with multimorbidity tend to engage significantly in ward and home medical care, often treat patients above 90 years, and employ numerous clinical strategies.
Efficient Synthesis and Comprehensive Characterization of bis‐Pyrazole Derivatives: Including X‐Ray Crystallography and Hirshfeld Surface Analysis
Authors: Boraei Ahmed T. A. , Soliman Saied M. , Haukka Matti , Barakat Assem , Sarhan Ahmed A. M. 
Published: September 2024

ABSTRACTStraightforward synthetic access to
bis
‐pyrazole derivatives has presented. The titled
bis
‐pyrazole derivative: 3′,5‐diphenyl‐1′,2‐
bis
(5,6,7,8‐tetrahydrobenzo[4,5]thieno[2,3‐d]pyrimidin‐4‐yl)‐1′H,2H‐[3,4′‐
bis
pyrazol]‐5′‐ol 3 was obtained from the reaction of pyran‐2,4‐dione 1 and 4‐hydrazineyl‐5,6,7,8‐tetrahydrobenzo[4,5]thieno[2,3‐d]pyrimidine 2 in ethanol in a one‐step reaction. The other
bis‐pyrazole derivative: 5,5′‐diphenyl‐1′H,2H‐[3,4′‐bispyrazol]‐3′‐ol 5 formed from hydrazinolysis of pyran‐2,4‐dione 1 or (E)‐3‐((allylamino)(phenyl)methylene)‐6‐phenyl‐2H‐pyran‐2,4(3H)‐dione 4 in ethanol. The molecular structure of both compounds elucidated by X‐ray crystallographic identification and spectrophotometric tools. The supramolecular structure of 3 could be described as a hydrogen bonded dimer via O–H…N interactions which are further connected by π…π contacts. Hirshfeld analysis showed the significance of the N…H, O…H, C…H, C…C, N…N, C…O and H…H interactions. These contacts contributed by 14.4%, 3.2%, 16.4%, 3.9%, 1.0%, 0.4% and 42.7%, respectively from the whole interactions occurred in the crystal. The d
norm, shape index and curvedness maps revealed the importance of π–π stacking interactions in the molecular packing where the % C…C is 3.9%. In case of 5, the short N…H, C…H, and H…H contacts contributed by 15.5%–17.0%, 28.3%–36.7% and 37.8%–45%, respectively in the molecular packing.