bluuebunny/crossref_metadata_2025_split huggingface dataset:
Projections of Extreme Temperature–Related Deaths in the US
Authors: Khatana Sameed Ahmed M. , Szeto Jonathan J. , Eberly Lauren A. , Nathan Ashwin S. , Puvvula Jagadeesh , Chen Aimin 
Published: September 2024

ImportanceExtreme heat in the US is increasing due to climate change, while extreme cold is projected to decline. Understanding how extreme temperature along with demographic changes will affect population health is important for devising policies to mitigate the health outcome of climate change.ObjectiveTo assess the burden of extreme temperature–related deaths in the contiguous US currently (2008-2019) and estimate the burden in the mid–21st century (2036-2065).Design, Setting, and ParticipantsThis cross-sectional study used historical (1979-2000) daily mean temperatures to calculate monthly extreme heat (&amp;gt;97.5th percentile value) and extreme cold days (&amp;lt;2.5th percentile value) for all contiguous US counties for 2008 to 2019 (current period). Temperature projections from 20 climate models and county population projections were used to estimate extreme temperature–related deaths for 2036 to 2065 (mid–21st century period). Data were analyzed from November 2023 to July 2024.ExposureCurrent monthly frequency of extreme heat days and projected mid–21st century frequency using 2 greenhouse gas emissions scenarios: Shared Socioeconomic Pathway (SSP)2-4.5, representing socioeconomic development with a lower emissions increase, and SSP5-8.5, representing higher emissions increase.Main Outcomes and MeasuresMean annual estimated number of extreme temperature–related excess deaths. Poisson regression model with county, month, and year fixed effects was used to estimate the association between extreme temperature and monthly all-cause mortality for older adults (aged ≥65 years) and younger adults (aged 18-64 years).ResultsAcross the contiguous US, extreme temperature days were associated with 8248.6 (95% CI, 4242.6-12 254.6) deaths annually in the current period and with 19 348.7 (95% CI, 11 388.7-27 308.6) projected deaths in the SSP2-4.5 scenario and 26 574.0 (95% CI, 15 408.0-37 740.1) in the SSP5-8.5 scenario. The mortality data included 30 924 133 decedents, of whom 15 573 699 were males (50.4%), with 6.3% of Hispanic ethnicity, 11.5% of non-Hispanic Black race, and 79.3% of non-Hispanic White race. Non-Hispanic Black adults (278.2%; 95% CI, 158.9%-397.5%) and Hispanic adults (537.5%; 95% CI, 261.6%-813.4%) were projected to have greater increases in extreme temperature–related deaths from the current period to the mid–21st century period compared with non-Hispanic White adults (70.8%; 95% CI, −5.8% to 147.3%).Conclusions and RelevanceThis cross-sectional study found that extreme temperature–related deaths in the contiguous US were projected to increase substantially by mid–21st century, with certain populations, such as non-Hispanic Black and Hispanic adults, projected to disproportionately experience this increase. The results point to the need to mitigate the adverse outcome of extreme temperatures for population health.
RBPMS‐AS1 sponges miR‐19a‐3p to restrain cervical cancer cells via enhancing PLCL1‐mediated pyroptosis
Authors: Huang Lina , Shen Qinqin , Yu Kun , Yang Jie , Li Xiuxiu 
Published: September 2024

AbstractCervical cancer (CC) poses a threat to human health. Enhancing pyroptosis can prevent the proliferation and epithelial–mesenchymal transition (EMT) of tumor cells. This study aims to reveal the candidates that modulate pyroptosis in CC. Accordingly, the common microRNAs (miRNAs/miRs) that were sponged by RBPMS antisense RNA 1 (RBPMS‐AS1) and could target Phospholipase C–Like 1 (PLCL1) were intersected. The expression of PBPMS‐AS1/miR‐19a‐3p (candidate miRNA)/PLCL1 was predicted in cervical squamous cell carcinoma (CESC), by which the expression location of RBPMS‐AS1 and the binding between RBPMS‐AS1/PLCL1 and miR‐19a‐3p were analyzed. The targeting relationship between RBPMS‐AS1/PLCL1 and miR‐19a‐3p was confirmed by dual‐luciferase reporter assay. After the transfection, cell counting kit‐8 assay, colony formation assay, quantitative reverse transcription PCR, and Western blot were implemented for cell viability and proliferation analysis as well as gene and protein expression quantification analysis. Based on the results, RBPMS‐AS1 and PLCL1 were lowly expressed, yet miR‐19a‐3p was highly expressed in CESC. RBPMS‐AS1 overexpression diminished the proliferation and expressions of N‐cadherin, vimentin, and miR‐19a‐3p, yet enhanced those of E‐cadherin, PLCL1, and pyroptosis‐relevant proteins (inteleukin‐1β, caspase‐1, and gasdermin D N‐terminal). However, the above RBPMS‐AS1 overexpression–induced effects were counteracted in the presence of miR‐19a‐3p. There also existed a targeting relationship and negative interplay between PLCL1 and miR‐19a‐3p. In short, RBPMS‐AS1 sponges miR‐19a‐3p and represses the growth and EMT of CC cells via enhancing PLCL1‐mediated pyroptosis.
LipidOne 2.0: A Web Tool for Discovering Biological Meanings Hidden in Lipidomic Data
Authors: Alabed Husam B. R. , Mancini Dorotea Frongia , Buratta Sandra , Calzoni Eleonora , Giacomo Danika Di , Emiliani Carla , Martino Sabata , Urbanelli Lorena , Pellegrino Roberto Maria 
Published: September 2024

AbstractLipidOne 2.0 (https://lipidone.eu) is a new web bioinformatic tool for the analysis of lipidomic data. It facilitates the exploration of the three structural levels of lipids: classes, molecular species, and lipid building blocks (acyl, alkyl, or alkenes chains). The tool's flexibility empowers users to seamlessly include or exclude experimental groups and lipid classes at any stage of the analysis. LipidOne 2.0 offers a range of mono‐ and multivariate statistical analyses, specifically tailored to each structural level. This includes a novel lipid biomarker identification function, integrating four diverse statistical parameters. LipidOne 2.0 incorporates Lipid Pathway analysis across all three structural levels of lipids. Users can identify lipid‐involved reactions through case‐control comparisons, generating lists of genes/enzymes and their activation states based on Z scores. Accessible without the need for registration, LipidOne 2.0 provides a user‐friendly and efficient platform for exploring and analyzing lipidomic data. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Dataset preparation for LipidOne 2.0Support Protocol: Lipid nomenclature from spectrometric experimentsBasic Protocol 2: Uploading a dataset into LipidOne 2.0Basic Protocol 3: Data mining of lipidomic dataset by LipidOne 2.0
Clinical observations of weight stigma among nursing students: A descriptive approach
Authors: Kerbyson Myah , Clark Kristen D. 
Published: September 2024

AbstractBackgroundMistreatment of individuals due to their body size or weight stigma has been described as a source of stress and as a deterrent for engagement in the healthcare system. Efforts to reduce weight stigma have included curricular interventions to reduce weight bias among future healthcare professionals. However, few studies have examined students’ observations of weight stigma and subsequent socialization to healthcare environmental norms.AimsThe purpose of this study is to evaluate observed weight stigma among pre‐licensure nursing students and recent graduates during clinical rotations and its effect on the perceived ability to provide respectful care to people in larger bodies.Materials and MethodsA cross‐sectional, descriptive study was conducted from February to March 2023. Participants answered questions related to personal weight bias (Fat Phobia Scale), observations of weight stigma, and perceived ability to provide respectful care.ResultsNinety‐one participants completed the survey, of whom half (n = 48) held a high level of weight bias. A quarter of participants (n = 23) reported that they engaged in weight stigma behaviors and a quarter (n = 24) of participants observed weight stigma behaviors from two or more healthcare professional roles. Almost one‐third (n = 10) of participants who observed weight stigma behaviors perceived that it impacted their ability to provide respectful care to patients in larger bodies.ConclusionsThese findings describe that weight stigma enacted by multiple healthcare professional roles is observed by nursing students during clinical rotations and is perceived to impact their ability to provide respectful care. Efforts to improve healthcare professionals’ sensitivity to the effects of weight bias and weight stigma should include addressing the role of socialized norms on weight bias among future healthcare professionals.
Structural insights into type III polyketide synthase CylI from cylindrocyclophane biosynthesis
Authors: Wang Hua‐Qi , Xiang Zheng 
Published: September 2024

AbstractType III polyketide synthases (PKSs) catalyze the formation of a variety of polyketide natural products with remarkable structural diversity and biological activities. Despite significant progress in structural and mechanistic studies of type III PKSs in bacteria, fungi, and plants, research on type III PKSs in cyanobacteria is lacking. Here, we report structural and mechanistic insights into CylI, a type III PKS that catalyzes the formation of the alkylresorcinol intermediate in cylindrocyclophane biosynthesis. The crystal structure of apo‐CylI reveals a distinct arrangement of structural elements that are proximal to the active site. We further solved the crystal structures of CylI in complexes with two substrate analogues at resolutions of 1.9 Å. The complex structures indicate that N259 is the key residue that determines the substrate preference of CylI. We also solved the crystal structure of CylI complexed with the alkylresorcinol product at a resolution of 2.0 Å. Structural analysis and mutagenesis experiments suggested that S170 functions as a key residue that determines cyclization specificity. On the basis of this result, a double mutant was engineered to completely switch the cyclization of CylI from aldol condensation to lactonization. This work elucidates the molecular basis of type III PKS in cyanobacteria and lays the foundation for engineering CylI‐like enzymes to generate new products.
An Intelligent Hydrogel Platform with Triple‐Triggered On‐Demand Release for Accelerating Diabetic Wound Healing
Authors: Zhang Xueliang , Li Yingying , Zhao Zhenfang , Ding Jin , Shan Haojie , Ren Ruizhen , Du Chang 
Published: September 2024

AbstractThe hydrogel platform with intelligent drug delivery system possesses great potential in the treatment of diabetic wounds. Nevertheless, the intelligent elimination of reactive oxygen species (ROS) remains a formidable challenge in facilitating diabetic wound healing. Herein, a hydrogel platform with triple‐triggered on‐demand release is constructed to intelligently scavenge ROS and modulate the wound microenvironment to accelerate diabetic wound healing through the release of antioxidative factors. Specifically, the gelatin (Gel) is modified with phenylboronic acid (PBA) to obtain a glucose‐sensitive Gel derivative (Gel‐BA), which is mixed with oxidized dextran (ODex) and the strong antioxidant myricetin (MY) to swiftly generate a hydrogel platform (OGM). Significantly, the smart release of MY from the hybrid hydrogel under inflammatory conditions intelligently eliminates ROS, effectively alleviating oxidative stress and promoting angiogenic reprogramming of the wound immune microenvironments by activating the Nrf2 pathway. In summary, in vitro and in vivo studies reveal that the OGM hydrogel platform significantly promotes cell proliferation, migration, and tube formation and greatly accelerates diabetic wound healing, offering a local‐specific triple‐response drug release strategy for the treatment of diabetic wound management.
Exploring Intersectional Perspectives in Software Engineering Through Narratives
Authors: Sánchez-Gordón Mary , Colomo-Palacios Ricardo 
Published: September 2024

AbstractIn this chapter, we explore intersectional perspectives in software engineering by collecting ethnographic histories from three software practitioners from underrepresented groups (URGs). The chapter draws attention to multiple interlocking issues related to diversity and aims to help software engineering researchers and practitioners to become more aware of conscious and unconscious discrimination and to re-story interactions with members of URGs. Without diversity, majoritarian perspectives dominate the software engineering field. This chapter begins with an introduction to intersectionality followed by our research approach, and then three disruptive majoritarian narratives are presented.
Rethinking Gender Diversity and Inclusion Initiatives for CS and SE in a University Setting
Authors: Simmonds Jocelyn , Bastarrica María Cecilia , Hitschfeld Nancy , Mascaró Pablo Villar , Wistuba La Torre Diego S. , Montenegro Jo Muñoz , Cárdenas Millaray 
Published: September 2024

AbstractJocelyn Simmonds, María Cecilia Bastarrica, Nancy Hitschfeld-Kahler, Pablo Villar Mascaró, Diego S. Wistuba La Torre, Jo Muñoz Montenegro, and Millaray Cárdenas
How to Measure Diversity Actionably in Technology
Authors: Hamid Md Montaser , Chatterjee Amreeta , Guizani Mariam , Anderson Andrew , Moussaoui Fatima , Yang Sarah , Escobar Isaac , Sarma Anita , Burnett Margaret 
Published: September 2024

AbstractMd Montaser Hamid, Amreeta Chatterjee, Mariam Guizani, Andrew Anderson, Fatima Moussaoui, Sarah Yang, Isaac Tijerina Escobar, Anita Sarma, and Margaret Burnett
Elicitation Revisited for More Inclusive Requirements Engineering
Authors: Tizard James , Rietz Tim , Blincoe Kelly 
Published: September 2024

AbstractJames Tizard, Tim Rietz, and Kelly BlincoeTo create inclusive software, development teams need to consider how they identify inclusive requirements for a software product. Requirements elicitation is the first stage in the process of developing the requirements of a software system. Elicitation is about describing the functionality, reliability, efficiency, and usability of the system to be developed, so that it suits the end users’ needs [12].Recent research has found that both traditional elicitation techniques (e.g., user interviews) and newer online crowd-based approaches may have challenges in gathering the views of a diverse set of users. In particular, there are significant challenges in eliciting requirements from users with cognitive disabilities, as well as ensuring that the full demographic spectrum (e.g., by gender, age, ethnicity) of a user base is adequately represented.This chapter discusses the motivations for more inclusive requirements elicitation and the challenges that need to be overcome and finally makes recommendations for both requirements engineering practitioners and researchers.
The role of fibre bonds in permanent curl of paper and how it is affected by crosslinking
Authors: Maaß Alexander , Selinger Julian , Hirn Ulrich 
Published: September 2024

AbstractIrreversible deformation of paper is a challenge for both printer operation and product quality, particularly in inkjet printing with water-based inks. Here we are investigating permanent paper curl, which is the residual curl of paper after drying of the ink (i.e., it is not the immediate paper curl due to wetting). The key aim of this work was to test the hypothesis that permanent paper curl is created by partial opening and rearrangement of the fibre–fibre bonds in the wetted paper layer. In order to test this hypothesis, we produced paper with crosslinked fibre–fibre bonds that do not open in the presence of water. Polyamideamine epichlorohydrin (PAE) and 1,2,3,4-butanetetracarboxylic acid were used as crosslinking agents and properties of the treated paper samples were analysed. Both agents led to significantly improved wet strength of the papers, furthermore we indeed found that the permanent curl of crosslinked papers was strongly reduced. Other curl related mechanisms like differences in fibre swelling, paper hydroexpansion and liquid penetration were not able to explain the reduction in curl. The finding that the creation of fibre–fibre bonds unaffected by water prevents permanent curl of paper after wetting and redrying leads to the conclusion that the mechanism for creating permanent paper curl after wetting is related to the partial opening and rearrangement of fibre–fibre bonds in the wetted paper. Possible pathways to apply these findings to paper production are discussed, for example switchable or temporary wet strength agents.
Imaging of the skull base and orbital tumors
Authors: Sakai Masafumi , Hiyama Takashi , Kuno Hirofumi , Kobayashi Tatsushi , Nakajima Takahito 
Published: September 2024

Abstract
The skull base and orbit have complicated anatomical structures where various tumors can occur. The tumor may present with neurological symptoms; however, its diagnosis is clinically difficult owing to accessibility issues. Therefore, diagnostic imaging is crucial in assessing tumors in the skull base and orbit and guiding subsequent management. Notably, some tumors have a predilection for a specific site of origin, and identifying the site of origin on imaging can help narrow the differential diagnosis. At the skull base, chordomas typically occur in the clivus, chondrosarcomas in the paramedian areas, paragangliomas in the jugular foramen, neurogenic tumors, and perineural spread in the neural foramen. Among orbital tumors, cavernous hemangiomas usually occur in the intraconal space, and pleomorphic adenomas and adenoid cystic carcinomas occur in the lacrimal glands. Some skull base and orbital tumors exhibit distinctive imaging features. Chordomas and chondrosarcomas of the skull base show high signal intensities on T2-weighted images, with chondrosarcomas often displaying cartilaginous calcifications. Paragangliomas are characterized by their hypervascular nature. In the orbit, cavernous hemangiomas and pleomorphic adenomas present unique dynamic patterns. Immunoglobulin G4-related disease forms lesions along the nerves. Identifying the tumor origin and its imaging characteristics can help narrow the differential diagnosis of skull base and orbital tumors.
Misorientations and Subgrains in Sn-Ag and Sn-Ag-Cu Solder Balls After Solidification
Authors: Sun Sihan , Xian Jingwei , Hsieh Chen-Lin , Gourlay Christopher M. 
Published: September 2024

AbstractSubgrains and recrystallisation are common microstructural features in solder joints that have been subjected to thermal fatigue or mechanical loading. Here we study similar features in Sn-Ag and Sn-Ag-Cu solder balls after solidification. It is shown that four types of misorientation features exist to different extents in solder balls examined shortly after solidification: (i) small variations in orientation created by dendrite growth and eutectic solidification, (ii) partial polygonisation into subgrains, (iii) small grains with high angle boundaries and (iv) large anomalous grains surrounded by interlacing where the grain boundaries do not correlate with the dendrite growth pattern. The subgrains often have a boundary plane and rotation axis consistent with dislocations from a facile slip system. The recovery and misorientation features were more extensive in regions that solidified at deeper melt undercooling. The findings highlight the importance of distinguishing between the solidification and solid-state components of microstructure evolution when interpreting solder microstructures after solidification.
Can Physicochemical Properties Alter the Potency of Aeroallergens? Part 2 – Impact of Physicochemical Properties
Authors: Teixeira Carla S. S. , Carriço-Sá Bruno , Villa Caterina , Mafra Isabel , Costa Joana 
Published: September 2024

Abstract
Purpose of Review
A holistic perspective on how physicochemical properties modulate the allergenicity of proteins has recently been performed for food allergens, launching the challenge of a similar analysis for aeroallergens. After a first review on aeroallergen classification into protein families (Part 1), this second part (Part 2) will exploit the impact of physicochemical properties (abundance/biological function, protein structure/presence of post-translational modifications, ligand/cofactor/lipid-binding) on inhalant protein allergenicity.

Recent Findings
The abundance linked to biological function is correlated with increased allergenic risk for most protein families, while the loss of structural integrity with consequent destruction of conformational epitopes is well linked with decreased allergenicity. Ligand-binding effect totally depends on the ligand type being highly variable among aeroallergens.

Summary
Knowledge about the physicochemical properties of aeroallergens is still scarce, which highlights the need for research using integrated approaches (in silico and experimental) to generate and analyze new data on known/new aeroallergens.
Evaluation of the 10-item Mental Toughness Questionnaire (MTQ10): cross-cultural assessment and scrutiny of method effects
Authors: Denovan Andrew , Dagnall Neil , Artamonova Elena , Papageorgiou Kostas A. 
Published: September 2024

AbstractThe 10-item Mental Toughness Questionnaire (MTQ10) is an easy to administer, global measure of mental toughness. Previous analysis established that the MTQ10 was psychometrically superior to the preceding, widely used, 18-item version. Nevertheless, the MTQ10 is potentially undermined by a method effect arising from the inclusion of negatively phrased items. Noting this, the present paper further assessed the measurement properties of the MTQ10 via cross-cultural comparisons. This involved assessing the factor composition in samples from the UK (N = 596), Greece (N = 1230), and Italy (N = 425). Rather than a strict method effect, analyses found effects specific to pairs of negative items. The emergent model demonstrated partial invariance across countries. National variations in mental toughness scores were ascribable to societal differences. Convergent validity was demonstrated using theoretically related variables (Dark Triad and Life Satisfaction). Overall, results supported the use of the MTQ10 as a global mental toughness measure. Additionally, outcomes suggested that further cross-cultural comparison would provide useful insights into the nature of mental toughness.
The fluidity of empire: hydraulics of neo-assyrian canal systems in relation to their possible uses
Authors: Stampoultzidis A. , Morandi Bonacossi D. , Reculeau H. , Savioli A. , Van Nooijen R. R. P. , Ertsen M. W. 
Published: September 2024

AbstractThe Neo-Assyrian empire offers a clear example of the key relationship between imperial power and water management. Irrigated landscapes under the direct impulse of the Assyrian state were important during its imperial heydays of the late 9th to 7th centuries BC. In the very heartland of the empire (the “Assur-Nineveh-Arbela Triangle” this imperialization of the landscape was the most dramatic, with large canals, aqueducts, and dams redirecting water flows toward the urban areas. Our hydraulic analysis of the canal network connecting Nineveh to its northeastern hinterlands explores how large-scale imperial investments generated imperialized landscapes that marked a substantial divergence from the preexisting conditions in terms of shape and sizes of canal infrastructure. One key question is whether Sennacherib’s hydraulic accomplishments provided meaningful changes to the agricultural economy of the region surrounding Nineveh, especially whether the canals allowed shifting the focus from extensive dry farming to intensive irrigation farming. Our hydraulic modelling shows that Assyrian water controllers and users would benefit from irrigation on the fields upstream of Nineveh. However, they would not benefit automatically from more control of flows in and from the system. Our results do not provide a single, definitive answer, given the uncertainty in data and the many options to model and understand the water systems, but especially for inflows below reference levels, the importance of controlled irrigation is evident. This suggests that irrigation will have been very likely to increase yields throughout the canal area.