bluuebunny/crossref_metadata_2025_split huggingface dataset:
Twin pregnancy in a Fontan-palliated patient
Authors: Nair Anupama , Radhakrishnan Sitaraman , Iyer Krishna S. 
Published: April 2016

AbstractThe Fontan connection, originally described in 1971, is used to provide palliation for patients with many forms of CHDs that cannot support a biventricular circulation. An increasing number of females who have undergone these connections in childhood are now surviving into adulthood and some are becoming pregnant. We report a case of a 29-year-old woman who presented with a twin pregnancy at 33 weeks of gestation. She had significant deterioration of her cardiovascular status before the twin babies were delivered by emergency caesarean section owing to associated obstetric complications. This report also highlights the various maternal and fetal complications occurring in pregnancy of Fontan-palliated patients and suggests the need for meticulous pre-conception counselling and strict perinatal care.
Marriage, Family and Politics: The Ilkhanid-Oirat Connection
Authors: BROADBRIDGE ANNE F. 
Published: April 2016

AbstractThe Chinggisids clearly favoured specific in-law clans through policies of repeated marriage over generations. This paper charts the fortunes of one such clan, the Oirats, who first joined the Chinggisids when Chinggis Khan and Börte's daughter Chechiyegen wedded an Oirat prince. Thereafter Chechiyegen's own daughters married back to the Toluid, Jochid and Chagatayid families. This paper follows those Oirats who intermarried with the Ilkhanids, beginning with Chechiyegen's daughter Güyük and ending with the Toluid-Oirat Ilkhan, Abū Sa‘īd (r. 1317-1335).
Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint
Authors: Petsalaki Eleni , Zachos George 
Published: April 2016

AbstractWhen chromatin is trapped at the intercellular bridge, cells delay completion of cytokinesis (abscission) to prevent chromosome breakage. Here we show that inhibition of Cdc-like kinases (Clks) 1, 2 or 4 accelerates midbody resolution in normally segregating cells and correlates with premature abscission, chromatin breakage and generation of DNA damage in cytokinesis with trapped chromatin. Clk1, Clk2 and Clk4 localize to the midbody in an interdependent manner, associate with Aurora B kinase and are required for Aurora B–serine 331 (S331) phosphorylation and complete Aurora B activation in late cytokinesis. Phosphorylated Aurora B–S331 localizes to the midbody centre and is required for phosphorylation and optimal localization of the abscission protein Chmp4c. In addition, expression of phosphomimetic mutants Aurora B–S331E or Chmp4c-S210D delays midbody disassembly and prevents chromatin breakage in Clk-deficient cells. We propose that Clks 1, 2 and 4 impose the abscission checkpoint by phosphorylating Aurora B–S331 at the midbody.
Potential pathways by which maternal second-hand smoke exposure during pregnancy causes full-term low birth weight
Authors: Niu Zhongzheng , Xie Chuanbo , Wen Xiaozhong , Tian Fuying , Yuan Shixin , Jia Deqin , Chen Wei-Qing 
Published: April 2016

AbstractIt is well documented that maternal exposure to second-hand smoke (SHS) during pregnancy causes low birth weight (LBW), but its mechanism remains unknown. This study explored the potential pathways. We enrolled 195 pregnant women who delivered full-term LBW newborns and 195 who delivered full-term normal birth weight newborns as the controls. After controlling for maternal age, education level, family income, pre-pregnant body mass index, newborn gender and gestational age, logistic regression analysis revealed that LBW was significantly and positively associated with maternal exposure to SHS during pregnancy, lower placental weight, TNF-α and IL-1β and that SHS exposure was significantly associated with lower placental weight, TNF-α and IL-1β. Structural equation modelling identified two plausible pathways by which maternal exposure to SHS during pregnancy might cause LBW. First, SHS exposure induced the elevation of TNF-α, which might directly increase the risk of LBW by transmission across the placenta. Second, SHS exposure first increased maternal secretion of IL-1β and TNF-α, which then triggered the secretion of VCAM-1; both TNF-α and VCAM-1 were significantly associated with lower placental weight, thus increasing the risk of LBW. In conclusion, maternal exposure to SHS during pregnancy may lead to LBW through the potential pathways of maternal inflammation and lower placental weight.
Sequence diversity of NanA manifests in distinct enzyme kinetics and inhibitor susceptibility
Authors: Xu Zhongli , von Grafenstein Susanne , Walther Elisabeth , Fuchs Julian E. , Liedl Klaus R. , Sauerbrei Andreas , Schmidtke Michaela 
Published: April 2016

AbstractStreptococcus pneumoniae is the leading pathogen causing bacterial pneumonia and meningitis. Its surface-associated virulence factor neuraminidase A (NanA) promotes the bacterial colonization by removing the terminal sialyl residues from glycoconjugates on eukaryotic cell surface. The predominant role of NanA in the pathogenesis of pneumococci renders it an attractive target for therapeutic intervention. Despite the highly conserved activity of NanA, our alignment of the 11 NanAs revealed the evolutionary diversity of this enzyme. The amino acid substitutions we identified, particularly those in the lectin domain and in the insertion domain next to the catalytic centre triggered our special interest. We synthesised the representative NanAs and the mutagenized derivatives from E. coli for enzyme kinetics study and neuraminidase inhibitor susceptibility test. Via molecular docking we got a deeper insight into the differences between the two major variants of NanA and their influence on the ligand-target interactions. In addition, our molecular dynamics simulations revealed a prominent intrinsic flexibility of the linker between the active site and the insertion domain, which influences the inhibitor binding. Our findings for the first time associated the primary sequence diversity of NanA with the biochemical properties of the enzyme and with the inhibitory efficiency of neuraminidase inhibitors.
Reversal of the Detrimental Effects of Post-Stroke Social Isolation by Pair-Housing is Mediated by Activation of BDNF-MAPK/ERK in Aged Mice
Authors: Verma Rajkumar , Harris Nia M. , Friedler Brett D. , Crapser Joshua , Patel Anita R. , Venna Venugopal , McCullough Louise D. 
Published: April 2016

AbstractSocial isolation (SI) increases stroke-related mortality and morbidity in clinical populations. The detrimental effects of SI have been successfully modeled in the laboratory using young animals. Mechanistically, the negative effects of SI in young animals are primarily mediated by an enhanced inflammatory response to injury and a reduction in neurotrophic factors. However, the response to brain injury differs considerably in the aged. Given that SI is more prevalent in aged populations, we hypothesized that isolation, even when initiated after stroke, would delay recovery in aged mice. We found that aged isolated male mice had significantly increased infarct volume, neurological deficits and serum IL-6 levels three days after stroke compared to pair housed (PH) mice. Using RT2 Profiler PCR Array and real-time quantitative PCR we found several important synaptic plasticity genes were differentially expressed in post-stroke SI mice. Furthermore, paired mice showed improved memory and neurobehavioral recovery four weeks after injury. Mechanistic and histological studies showed that the beneficial effects of pair housing are partially mediated by BDNF via downstream MAPK/ERK signaling and restoration of axonal basic myelin protein levels.
Crystal growth and optical characteristics of beryllium-free polyphosphate, KLa(PO3)4, a possible deep-ultraviolet nonlinear optical crystal
Authors: Shan Pai , Sun Tongqing , Chen Hong , Liu Hongde , Chen Shaolin , Liu Xuanwen , Kong Yongfa , Xu Jingjun 
Published: April 2016

AbstractDeep-ultraviolet nonlinear optical crystals are of great importance as key materials in generating coherent light with wavelength below 200 nm through cascaded frequency conversion of solid-state lasers. However, the solely usable crystal in practice, KBe2BO3F2 (KBBF), is still commercially unavailable because of the high toxicity of beryllium-containing and the extreme difficulty of crystal growth. Here, we report the crystal growth and characteristics of an beryllium-free polyphosphate, KLa(PO3)4. Centimeter-sized single crystals have been easily obtained by the flux method and slow-cooling technique. The second-harmonic generation efficiency of KLa(PO3)4 powder is 0.7 times that of KH2PO4; moreover, the KLa(PO3)4 crystal is phase-matchable. Remarkably, the KLa(PO3)4 crystal exhibits an absorption edge of 162 nm, which is the shortest among phase-matchable phosphates so far. These attributes make KLa(PO3)4 a possible deep-ultraviolet nonlinear optical crystal. An analysis of the dipole moments of the polyhedra and theoretical calculations by density functional theory were made to elucidate the structure-properties relationships of KLa(PO3)4.
Needle-array to Plate DBD Plasma Using Sine AC and Nanosecond Pulse Excitations for Purpose of Improving Indoor Air Quality
Authors: Zhang Li , Yang Dezheng , Wang Wenchun , Wang Sen , Yuan Hao , Zhao Zilu , Sang Chaofeng , Jia Li 
Published: April 2016

AbstractIn this study, needle-array to plate electrode configuration was employed to generate an atmospheric air diffuse discharge using both nanosecond pulse and sine AC voltage as excitation voltage for the purpose of improving indoor air quality. Different types of voltage sources and electrode configurations are employed to optimize electrical field distribution and improve discharge stability. Discharge images, electrical characteristics, optical emission spectra and plasma gas temperatures in both sine AC discharge and nanosecond pulse discharge were compared and the discharge stability during long operating time were discussed. Compared with the discharge excited by sine AC voltage, the nanosecond pulsed discharge is more homogenous and stable, besides, the plasma gas temperature of nanosecond pulse discharge is much lower. Using packed-bed structure, where γ- Al2O3 pellets are filled in the electrode gap, has obvious efficacy in the production of homogenous discharge. Furthermore, both sine AC discharge and nanosecond pulse discharge were used for removing formaldehyde from flowing air. It shows that nanosecond pulse discharge has a significant advantage in energy cost. And the main physiochemical processes for the generation of active species and the degradation of formaldehyde were discussed.
Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring
Authors: Jiang Zhou , Li Ping , Van Nostrand Joy D. , Zhang Ping , Zhou Jizhong , Wang Yanhong , Dai Xinyue , Zhang Rui , Jiang Dawei , Wang Yanxin 
Published: April 2016

AbstractAlkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study arsenic geochemistry and microbial community using Illumina MiSeq sequencing. Over 0.26 million 16S rRNA sequence reads were obtained from 5-paired parallel water and sediment samples along the hot spring’s outflow channel. High ratios of As(V)/AsSum (total combined arsenate and arsenite concentrations) (0.59–0.78), coupled with high sulfide (up to 5.87 mg/L), were present in the hot spring’s pools, which suggested As(III) oxidation occurred. Along the outflow channel, AsSum increased from 5.45 to 13.86 μmol/L, and the combined sulfide and sulfate concentrations increased from 292.02 to 364.28 μmol/L. These increases were primarily attributed to thioarsenic transformation. Temperature, sulfide, As and dissolved oxygen significantly shaped the microbial communities between not only the pools and downstream samples, but also water and sediment samples. Results implied that the upstream Thermocrinis was responsible for the transformation of thioarsenic to As(III) and the downstream Thermus contributed to derived As(III) oxidation. This study improves our understanding of microbially-mediated As transformation in alkaline sulfide-rich hot springs.
In Vivo Mesoscopic Voltage-Sensitive Dye Imaging of Brain Activation
Authors: Tang Qinggong , Tsytsarev Vassiliy , Frank Aaron , Wu Yalun , Chen Chao-wei , Erzurumlu Reha S. , Chen Yu 
Published: April 2016

AbstractFunctional mapping of brain activity is important in elucidating how neural networks operate in the living brain. The whisker sensory system of rodents is an excellent model to study peripherally evoked neural activity in the central nervous system. Each facial whisker is represented by discrete modules of neurons all along the pathway leading to the neocortex. These modules are called “barrels” in layer 4 of the primary somatosensory cortex. Their location (approximately 300–500 μm below cortical surface) allows for convenient imaging of whisker-evoked neural activity in vivo. Fluorescence laminar optical tomography (FLOT) provides depth-resolved fluorescence molecular information with an imaging depth of a few millimeters. Angled illumination and detection configurations can improve both resolution and penetration depth. We applied angled FLOT (aFLOT) to record 3D neural activities evoked in the whisker system of mice by deflection of a single whisker in vivo. A 100 μm capillary and a pair of microelectrodes were inserted to the mouse brain to test the capability of the imaging system. The results show that it is possible to obtain 3D functional maps of the sensory periphery in the brain. This approach can be broadly applicable to functional imaging of other brain structures.
Role of Fusobacteria in the serrated pathway of colorectal carcinogenesis
Authors: Park Chan Hyuk , Han Dong Soo , Oh Young-Ha , Lee A-reum , Lee Yu-ra , Eun Chang Soo 
Published: April 2016

AbstractFusobacteria are associated with colorectal cancer (CRC) and are amplified during colorectal carcinogenesis. Compared to the adenoma-carcinoma sequence of carcinogenesis, serrated neoplasm has distinct clinical features and a different molecular background. We aimed to compare the gut microbiome between tubular adenoma (TA) and sessile serrated adenoma/polyp (SSA/P). Patients with TA, SSA/P, or CRC were recruited. Three pieces of colorectal mucosal tissue were obtained from each patient by endoscopic biopsy. 16S rRNA gene pyrosequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were performed. Among 26 enrolled patients, 8, 10, and 8 had TA, SSA/P, and CRC, respectively. The relative abundance of Fusobacteria did not differ significantly between the TA and SSA/P groups (4.3% and 1.9%, P = 0.739) but was higher in the CRC group (33.8%) than in the TA or SSA/P group, respectively (TA vs. CRC, P = 0.002, false discovery rate [FDR] = 0.023; SSA/P vs. CRC, P < 0.001, FDR = 0.001). PICRUSt revealed that most functions in the TA metagenome were similar to those in the SSA/P metagenome. The gut microbiome, including relative abundance of Fusobacteria, did not differ between TA and SSA/P, suggesting that Fusobacteria may contribute to both the serrated pathway and the adenoma-carcinoma sequence.
Transformation seismology: composite soil lenses for steering surface elastic Rayleigh waves
Authors: Colombi Andrea , Guenneau Sebastien , Roux Philippe , Craster Richard V. 
Published: April 2016

AbstractMetamaterials are artificially structured media that exibit properties beyond those usually encountered in nature. Typically they are developed for electromagnetic waves at millimetric down to nanometric scales, or for acoustics, at centimeter scales. By applying ideas from transformation optics we can steer Rayleigh-surface waves that are solutions of the vector Navier equations of elastodynamics. As a paradigm of the conformal geophysics that we are creating, we design a square arrangement of Luneburg lenses to reroute Rayleigh waves around a building with the dual aim of protection and minimizing the effect on the wavefront (cloaking). To show that this is practically realisable we deliberately choose to use material parameters readily available and this metalens consists of a composite soil structured with buried pillars made of softer material. The regular lattice of inclusions is homogenized to give an effective material with a radially varying velocity profile and hence varying the refractive index of the lens. We develop the theory and then use full 3D numerical simulations to conclusively demonstrate, at frequencies of seismological relevance 3–10 Hz, and for low-speed sedimentary soil (vs: 300–500 m/s), that the vibration of a structure is reduced by up to 6 dB at its resonance frequency.
A computational model of a human single sinoatrial node cell
Authors: Pohl A , Wachter A , Hatam N , Leonhardt S 
Published: April 2016

Abstract
For the investigation of the spontaneous rhythmical activity response in the application of cardiac neuromodulation, we formulated a human sinoatrial node (SAN) cell model. With the aim of decreasing elevated heart rate (HR), we want to establish a hardware-in-the-loop system including this model for the analysis of optimal stimulation patterns of the neurostimulation system. Base model structures are adopted from rabbit SAN cell models available in literature and conveyed with Hodgkin–Huxley-type model equations describing the complex time and voltage dependent activation and deactivation processes of individual ion channels. The resulting model consists of 15 currents which are currently known to be responsible for the generation of the membrane action potential (AP). The model reproduces AP frequencies equivalent to those measured in isolated human SAN cells with a resulting HR of 71.8 bpm. Model validation via simulation of the inhibitory effect of ivabradine showed accordance with experimental results obtained in human studies. Furthermore, we could validate the model in regard to its HR effects upon parasympathetic stimulation with results obtained in a human trial study.
Anthropomorphized Helpers Undermine Autonomy and Enjoyment in Computer Games
Authors: Kim Sara , Chen Rocky Peng , Zhang Ke 
Published: April 2016

AbstractAlthough digital assistants with humanlike features have become prevalent in computer games, few marketing studies have demonstrated the psychological mechanisms underlying consumers’ reactions to digital assistants and their subsequent influence on consumers’ game enjoyment. To fill this gap, the current study examined the effect of anthropomorphic representations of computerized helpers in computer games on game enjoyment. In the current research, consumers enjoyed a computer game less when they received assistance from a computerized helper imbued with humanlike features than from a helper construed as a mindless entity. We offer a novel mechanism that the presence of an anthropomorphized helper can undermine individuals’ perceived autonomy during a computer game. Across six experiments, we show that the presence of an anthropomorphized helper reduced game enjoyment across three different games. By measuring participants’ perceived autonomy (study 1) and employing moderators such as importance of autonomy (studies 2, 3, and 4), we also provide evidence that the reduced feeling of autonomy serves as the mechanism underlying the backfiring effect. Finally, we demonstrate that the effect of anthropomorphism on game enjoyment can be extended to other game-related outcomes, such as individuals’ motivation to persist in the game (studies 4 and 5).
Evidence that widespread star formation may be underway in G0.253+0.016, ‘The Brick’
Authors: Marsh K. A. , Ragan S. E. , Whitworth A. P. , Clark P. C. 
Published: April 2016

Abstract
Image cubes of differential column density as a function of dust temperature are constructed for Galactic Centre molecular cloud G0.253+0.016 (‘The Brick’) using the recently described PPMAP procedure. The input data consist of continuum images from the HerschelSpace Telescope in the wavelength range 70–500 μm, supplemented by previously published interferometric data at 1.3 mm wavelength. While the bulk of the dust in the molecular cloud is consistent with being heated externally by the local interstellar radiation field, our image cube shows the presence, near one edge of the cloud, of a filamentary structure whose temperature profile suggests internal heating. The structure appears as a cool (∼14 K) tadpole-like feature, ∼6 pc in length, in which is embedded a thin spine of much hotter (∼40–50 K) material. We interpret these findings in terms of a cool filament whose hot central region is undergoing gravitational collapse and fragmentation to form a line of protostars. If confirmed, this would represent the first evidence of widespread star formation having started within this cloud.
Genetic and epigenetic determinants of B-cell lymphoma evolution
Authors: Izzo Franco , Landau Dan A. 
Published: April 2016

Purpose of reviewThe success of targeted therapies fostered the development of increasingly specific and effective therapeutics for B-cell malignancies. However, cancer plasticity facilitates disease relapse, whereby intratumoral heterogeneity fuels tumor evolution into a more aggressive and resistant form. Understanding cancer heterogeneity and the evolutionary processes underlying disease relapse is key for overcoming this limitation of current treatment strategies. In the present review, we delineate the current understanding of cancer evolution and the advances in both genetic and epigenetic fields, with a focus on non-Hodgkin B-cell lymphomas.Recent findingsThe use of massively parallel sequencing has provided insights into tumor heterogeneity, allowing determination of intratumoral genetic and epigenetic variability and identification of cancer driver mutations and (epi-)mutations. Increased heterogeneity prior to treatment results in faster disease relapse, and in many cases studying pretreatment clonal admixtures predicts the future evolutionary trajectory of relapsed disease.SummaryUnderstanding the mechanisms underlying tumor heterogeneity and evolution provides valuable tools for the design of therapy within an evolutionary framework. This framework will ultimately aid in accurately predicting the evolutionary paths of B-cell malignancies, thereby guiding therapeutic strategies geared at directly anticipating and addressing cancer evolution.