bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coat Protein Mutations That Alter the Flux of Morphogenetic Intermediates through the ϕX174 Early Assembly Pathway
Authors: Blackburn Brody J. , Li Shuaizhi , Roznowski Aaron P. , Perez Alexis R. , Villarreal Rodrigo H. , Johnson Curtis J. , Hardy Margaret , Tuckerman Edward C. , Burch April D. , Fane Bentley A. 
Published: October 2017

ABSTRACT
Two scaffolding proteins orchestrate ϕX174 morphogenesis. The internal scaffolding protein B mediates the formation of pentameric assembly intermediates, whereas the external scaffolding protein D organizes 12 of these intermediates into procapsids. Aromatic amino acid side chains mediate most coat-internal scaffolding protein interactions. One residue in the internal scaffolding protein and three in the coat protein constitute the core of the B protein binding cleft. The three coat gene codons were randomized separately to ascertain the chemical requirements of the encoded amino acids and the morphogenetic consequences of mutation. The resulting mutants exhibited a wide range of recessive phenotypes, which could generally be explained within a structural context. Mutants with phenylalanine, tyrosine, and methionine substitutions were phenotypically indistinguishable from the wild type. However, tryptophan substitutions were detrimental at two sites. Charged residues were poorly tolerated, conferring extreme temperature-sensitive and lethal phenotypes. Eighteen lethal and conditional lethal mutants were genetically and biochemically characterized. The primary defect associated with the missense substitutions ranged from inefficient internal scaffolding protein B binding to faulty procapsid elongation reactions mediated by external scaffolding protein D. Elevating B protein concentrations above wild-type levels via exogenous, cloned-gene expression compensated for inefficient B protein binding, as did suppressing mutations within gene B. Similarly, elevating D protein concentrations above wild-type levels or compensatory mutations within gene D suppressed faulty elongation. Some of the parental mutations were pleiotropic, affecting multiple morphogenetic reactions. This progressively reduced the flux of intermediates through the pathway. Accordingly, multiple mechanisms, which may be unrelated, could restore viability.

IMPORTANCE
Genetic analyses have been instrumental in deciphering the temporal events of many biochemical pathways. However, pleiotropic effects can complicate analyses. Vis-à-vis virion morphogenesis, an improper protein-protein interaction within an early assembly intermediate can influence the efficiency of all subsequent reactions. Consequently, the flux of assembly intermediates cumulatively decreases as the pathway progresses. During morphogenesis, ϕX174 coat protein participates in at least four well-defined reactions, each one characterized by an interaction with a scaffolding or structural protein. In this study, genetic analyses, biochemical characterizations, and physiological assays, i.e., elevating the protein levels with which the coat protein interacts, were used to elucidate pleiotropic effects that may alter the flux of intermediates through a morphogenetic pathway.
Validity of triage systems for paediatric emergency care: a systematic review
Authors: de Magalhães-Barbosa Maria Clara , Robaina Jaqueline Rodrigues , Prata-Barbosa Arnaldo , Lopes Claudia de Souza 
Published: October 2017

AimTo present a systematic review on the validity of triage systems for paediatric emergency care.MethodsSearch in MEDLINE, Cochrane Library, Latin American and Caribbean Health Sciences Literature (LILACS), Scientific Electronic Library Online (SciELO), Nursing Database Index (BDENF) and Spanish Health Sciences Bibliographic Index (IBECS) for articles in English, French, Portuguese or Spanish with no time limit. Validity studies of five-level triage systems for patients 0–18 years old were included. Two reviewers performed data extraction and quality assessment as recommended by PRISMA statement.ResultsWe found 25 studies on seven triage systems: Manchester Triage System (MTS); paediatric version of Canadian Triage and Acuity Scale (PedCTAS) and its adaptation for Taiwan (paediatric version of the Taiwan Triage and Acuity System); Emergency Severity Index version 4 (ESI v.4); Soterion Rapid Triage System and South African Triage Scale and its adaptation for Bostwana (Princess Marina Triage Scale). Only studies on the MTS used a reference standard for urgency, while all systems were evaluated using a proxy outcome for urgency such as admission. Over half of all studies were low quality. The MTS, PedCTAS and ESI v.4 presented the largest number of moderate and high quality studies. The three tools performed better in their countries or near them, showing a consistent association with hospitalisation and resource utilisation. Studies of all three tools found that patients at the lowest urgency levels were hospitalised, reflecting undertriage.ConclusionsThere is some evidence to corroborate the validity of the MTS, PedCTAS and ESI v.4 for paediatric emergency care in their own countries or near them. Efforts to improve the sensitivity and to minimise the undertriage rates should continue. Cross-cultural adaptation is necessary when adopting these triage systems in other countries.
‘Nothing can be done until everything is done’: the use of complexity arguments by food, beverage, alcohol and gambling industries
Authors: Petticrew Mark , Katikireddi Srinivasa Vittal , Knai Cécile , Cassidy Rebecca , Maani Hessari Nason , Thomas James , Weishaar Heide 
Published: October 2017

BackgroundCorporations use a range of strategies to dispute their role in causing public health harms and to limit the scope of effective public health interventions. This is well documented in relation to the activities of the tobacco industry, but research on other industries is less well developed. We therefore analysed public statements and documents from four unhealthy commodity industries to investigate whether and how they used arguments about complexity in this way.MethodsWe analysed alcohol, food, soda and gambling industry documents and websites and minutes of reports of relevant health select committees, using standard document analysis methods.ResultsTwo main framings were identified: (i) these industries argue that aetiology is complex, so individual products cannot be blamed; and (ii) they argue that population health measures are ‘too simple’ to address complex public health problems. However, in this second framing, there are inherent contradictions in how industry used ‘complexity’, as their alternative solutions are generally not, in themselves, complex.ConclusionThe concept of complexity, as commonly used in public health, is also widely employed by unhealthy commodity industries to influence how the public and policymakers understand health issues. It is frequently used in response to policy announcements and in response to new scientific evidence (particularly evidence on obesity and alcohol harms). The arguments and language may reflect the existence of a cross-industry ‘playbook’, whose use results in the undermining of effective public health policies – in particular the undermining of effective regulation of profitable industry activities that are harmful to the public’s health.
Role of bronchodilation and pattern of breathing in increasing tidal expiratory flow with progressive induced hypercapnia in chronic obstructive pulmonary disease
Authors: Finucane Kevin E. , Singh Bhajan 
Published: October 2017

Hypercapnia (HC) in vitro relaxes airway smooth muscle; in vivo, it increases respiratory effort, tidal expiratory flows (V̇exp), and, by decreasing inspiratory duration (Ti), increases elastic recoil pressure (Pel) via lung viscoelasticity; however, its effect on airway resistance is uncertain. We examined the contributions of bronchodilation, Ti, and expiratory effort to increasing V̇exp with progressive HC in 10 subjects with chronic obstructive pulmonary disease (COPD): mean forced expiratory volume in 1 s (FEV1) 53% predicted. Lung volumes (Vl), V̇exp, esophageal pressure (Pes), Ti, and end-tidal Pco2 ([Formula: see text]) were measured during six tidal breaths followed by an inspiratory capacity (IC), breathing air, and at three levels of HC. V̇exp and V̇ with submaximal forced vital capacities breathing air (V̇sFVC) were compared. Pulmonary resistance ( Rl) was measured from the Pes-V̇ relationship. V̇exp and Pes at end-expiratory lung volume (EELV) + 0.3 tidal volume [V̇(0.3Vt) and Pes(0.3Vt), respectively], Ti, and Rl correlated with [Formula: see text] ( P < 0.001 for all) and were independent of tiotropium. [Formula: see text], Ti, and Pes(0.3Vt) predicted the increasing V̇(0.3Vt)/V̇sFVC(0.3Vt) [multiple regression analysis (MRA): P = 0.001, 0.004, and 0.025, respectively]. At [Formula: see text] ≥ 50 Torr, V̇(0.3Vt)/V̇sFVC(0.3Vt) exceeded unity in 30 of 36 measurements and was predicted by [Formula: see text] and Pes(0.3Vt) (MRA: P = 0.02 and 0.025, respectively). Rl decreased at [Formula: see text] 45 Torr ( P < 0.05) and did not change with further HC. IC and Vl(0.3Vt) did not change with HC. We conclude that in COPD HC increases V̇exp due to bronchodilation, increased Pel secondary to decreasing Ti, and increased expiratory effort, all promoting lung emptying and a stable EELV. NEW & NOTEWORTHY The response of airways to intrapulmonary hypercapnia (HC) is uncertain. In chronic obstructive pulmonary disease (COPD), progressive HC increases tidal expiratory flows by inducing bronchodilation and via an increased rate of inspiration and lung viscoelasticity, a probable increase in lung elastic recoil pressure, both changes increasing expiratory flows, promoting lung emptying and a stable end-expiratory volume. Bronchodilation with HC occurred despite optimal standard bronchodilator therapy, suggesting that in COPD further bronchodilation is possible.
Gamma Secretase Inhibition by BMS-906024 Enhances Efficacy of Paclitaxel in Lung Adenocarcinoma
Authors: Morgan Katherine M. , Fischer Bruce S. , Lee Francis Y. , Shah Jamie J. , Bertino Joseph R. , Rosenfeld Jeffrey , Singh Amartya , Khiabanian Hossein , Pine Sharon R. 
Published: October 2017

Abstract
Notch signaling is aberrantly activated in approximately one third of non–small cell lung cancers (NSCLC). We characterized the interaction between BMS-906024, a clinically relevant Notch gamma secretase inhibitor, and front-line chemotherapy in preclinical models of NSCLC. Chemosensitivity assays were performed on 14 human NSCLC cell lines. There was significantly greater synergy between BMS-906024 and paclitaxel than BMS-906024 and cisplatin [mean combination index (CI) value, 0.54 and 0.85, respectively, P = 0.01]. On an extended panel of 31 NSCLC cell lines, 25 of which were adenocarcinoma, the synergy between BMS-906024 and paclitaxel was significantly greater in KRAS- and BRAF-wildtype than KRAS- or BRAF-mutant cells (mean CI, 0.43 vs. 0.90, respectively; P = 0.003). Paclitaxel-induced Notch1 activation was associated with synergy between BMS-906024 and paclitaxel in the KRAS- or BRAF-mutant group. Knockdown of mutant KRAS increased the synergy between BMS-906024 and paclitaxel in heterozygous KRAS-mutant cell lines. Among KRAS- or BRAF-mutant NSCLC, there was a significant correlation between synergy and mutant or null TP53 status, as well as between synergy and a low H2O2 pathway signature. Exogenous overexpression of activated Notch1 or Notch3 had no effect on the enhanced sensitivity of NSCLC to paclitaxel by BMS-906024. In vivo studies with cell line– and patient-derived lung adenocarcinoma xenografts confirmed enhanced antitumor activity for BMS-906024 plus paclitaxel versus either drug alone via decreased cell proliferation and increased apoptosis. These results show that BMS-906024 sensitizes NSCLC to paclitaxel and that wild-type KRAS and BRAF status may predict better patient response to the combination therapy. Mol Cancer Ther; 16(12); 2759–69. ©2017 AACR.
Pharmacological Dual Inhibition of Tumor and Tumor-Induced Functional Limitations in a Transgenic Model of Breast Cancer
Authors: Wang Ruizhong , Bhat-Nakshatri Poornima , Padua Maria B. , Prasad Mayuri S , Anjanappa Manjushree , Jacobson Max , Finnearty Courtney , Sefcsik Victoria , McElyea Kyle , Redmond Rachael , Sandusky George , Penthala Narsimha , Crooks Peter A , Liu Jianguo , Zimmers Teresa , Nakshatri Harikrishna 
Published: October 2017

Abstract
Breast cancer progression is associated with systemic effects, including functional limitations and sarcopenia without the appearance of overt cachexia. Autocrine/paracrine actions of cytokines/chemokines produced by cancer cells mediate cancer progression and functional limitations. The cytokine-inducible transcription factor NF-κB could be central to this process, as it displays oncogenic functions and is integral to the Pax7:MyoD:Pgc-1β:miR-486 myogenesis axis. We tested this possibility using the MMTV-PyMT transgenic mammary tumor model and the NF-κB inhibitor dimethylaminoparthenolide (DMAPT). We observed deteriorating physical and functional conditions in PyMT+ mice with disease progression. Compared with wild-type mice, tumor-bearing PyMT+ mice showed decreased fat mass, impaired rotarod performance, and reduced grip strength as well as increased extracellular matrix (ECM) deposition in muscle. Contrary to acute cachexia models described in the literature, mammary tumor progression was associated with reduction in skeletal muscle stem/satellite-specific transcription factor Pax7. Additionally, we observed tumor-induced reduction in Pgc-1β in muscle, which controls mitochondrial biogenesis. DMAPT treatment starting at 6 to 8 weeks age prior to mammary tumor occurrence delayed mammary tumor onset and tumor growth rates without affecting metastasis. DMAPT overcame cancer-induced functional limitations and improved survival, which was accompanied with restoration of Pax7, Pgc-1β, and mitochondria levels and reduced ECM levels in skeletal muscles. In addition, DMAPT restored circulating levels of 6 out of 13 cancer-associated cytokines/chemokines changes to levels seen in healthy animals. These results reveal a pharmacological approach for overcoming cancer-induced functional limitations, and the above-noted cancer/drug-induced changes in muscle gene expression could be utilized as biomarkers of functional limitations. Mol Cancer Ther; 16(12); 2747–58. ©2017 AACR.
NRG Oncology/National Surgical Adjuvant Breast and Bowel Project Decision-Making Project-1 Results: Decision Making in Breast Cancer Risk Reduction
Authors: Holmberg Christine , Bandos Hanna , Fagerlin Angela , Bevers Therese B. , Battaglia Tracy A. , Wickerham D. Lawrence , McCaskill-Stevens Worta J. 
Published: October 2017

AbstractSelective estrogen receptor modulators (SERMs) reduce breast cancer risk. Adoption of SERMs as prevention medication remains low. This is the first study to quantify social, cultural, and psychologic factors driving decision making regarding SERM use in women counseled on breast cancer prevention options. A survey study was conducted with women counseled by a health care provider (HCP) about SERMs. A statistical comparison of responses was performed between those who decided to use and those who decided not to use SERMs. Independent factors associated with the decision were determined using logistic regression. Of 1,023 participants, 726 made a decision: 324 (44.6%) decided to take a SERM and 402 (55.4%) decided not to. The most important factor for deciding on SERM use was the HCP recommendation. Other characteristics associated with the decision included attitudes and perceptions regarding medication intake, breast cancer worry, trust in HCP, family members with blood clots, and others' experiences with SERMs. The odds of SERM intake when HCP recommended were higher for participants with a positive attitude toward taking medications than for those with a negative attitude (Pinteraction = 0.01). This study highlights the importance of social and cultural aspects for SERM decision making, most importantly personal beliefs and experiences. HCPs' recommendations play a statistically significant role in decision making and are more likely to be followed if in line with patients' attitudes. Results indicate the need for developing interventions for HCPs that not only focus on the presentation of medical information but, equally as important, on addressing patients' beliefs and experiences. Cancer Prev Res; 10(11); 625–34. ©2017 AACR.See related editorial by Crew, p. 609
Improving Efficiency of Power Systems by Demand Side Management Method
Authors: Symon V.A. Tibbie Pon , Ragland I. Jacob 
Published: October 2017

In the smart grid infrastructure based power systems, it is necessary to consider the demand side management to enhance the energy reduction and system control. In many countries the resources are very less so the available resources have to be used in an efficient manner without any loss. The total loss cannot be avoided but it can be reduced. In the proposed system, the Particle Swarm Optimization (PSO) technique is used to distribute the power in the smart grid. Here, the grids are arranged in such a way that the losses in it are reduced. The load connected to the grid is rearranged according to their use. It uses a new and stochastic scheduling technique to handle the uncertainties in the power system. Solar and wind power are taken in account for twenty four hours and the values are given to the PSO algorithm. The experiment was conducted by MATLAB and the results show that the efficiency level of wind and solar power systems was increased by an appreciable level. The proposed technique is compared with the normal system without using Demand Side Management (DSM) and it shows that the proposed system gives better results than the existing systems.
Nonlinear Compensation Empyoing Matrix Converter with DTC Controller
Authors: Gaied Khalaf S. , Salih Ziad H. , Ajel Ahmed R. , Marie Mehdi J. 
Published: October 2017

This paper describes a nonlinear harmful speed and torque controller for fourth order induction motor model. The investigation of optimality and cost function for that base on estimation of Hammerstein-Wiener model with the compensated mathematical model. The matrix converter with direct torque control combination is efficient way to get better performance specifications in the industry.The MC and the DTC advantages are combined together.The reduction of complexity and cost of DC link in the DTC since it has no capacitors in the circuit. However, the controlling torque is a big problem it in DTC because of high ripple torque production which results in vibrations response in the operation of the IM as it has no PID to control the torque directly. The combination of MC with DTC is applied to reduce the fluctuation in the output torque and minimize the steady state error. This paper presents the simulation analysis of induction machine drives using Maltlab/Simulink toolbox R2012a. Design of constant switching frequency MCDTC drive,stability investigation and fault protection as well as controllability and observability with minimum steady state error has been carried out which proved the effectiveness of the proposed technique.
PC-APC Schemes in Multipath Diversity System to Get Higher Throughput
Authors: Ningthoujam Sanjit , Dutta Manash Pratim , Banerjee Subhasish , Bhunia Chandan T. , Chakraborty Swarnendu K. 
Published: October 2017

This paper is studied about a new protocol of Packet combining (PC) and Aggressive Packet Combining Scheme (APC) in multipath diversity system to get higher throughput. In the proposed protocol of PC and APC schemes, two and three copies of a packet are sent in two and three paths. If either of the copies is received without any erroneous then select the correct one and discard all other copies. Again if all copies are found as erroneous then combine the erroneous copies and perform XOR operation in case of PC and bit by bit majority logic in case of APC in order to get the original copy. The paper is implemented using Mat lab and found that new proposed protocols are getting higher throughput and probability of receiving successful packet at the receiver side. Thus, performance of error prone wireless network can be improved by applying either PC or APC in multipath diversity system.
Streaming Big Data Analysis for Real-Time Sentiment based Targeted Advertising
Authors: Nair Lekha R. , Shetty Sujala D. , Deepak Shetty Siddhant 
Published: October 2017

Big Data constituting from the information shared in the various social network sites have great relevance for research to be applied in diverse fields like marketing, politics, health or disaster management. Social network sites like Facebook and Twitter are now extensively used for conducting business, marketing products and services and collecting opinions and feedbacks regarding the same. Since data gathered from these sites regarding a product/brand are up-to-date and are mostly supplied voluntarily, it tends to be more realistic, massive and reflects the general public opinion. Its analysis on real time can lead to accurate insights and responding to the results sooner is undoubtedly advantageous than responding later. In this paper, a cloud based system for real time targeted advertising based on tweet sentiment analysis is designed and implemented using the big data processing engine Apache Spark, utilizing its streaming library. Application is meant to promote cross selling and provide better customer support.
Prioritizing Power demand response for Hydrogen PEMFC-Electric Vehicles using Hybrid Energy Storage
Authors: Samir Jawadi , Sami Ben Slama , Adnane Cherif 
Published: October 2017

PEMFC powered Hybrid vehicle system is one of an interesting issue for the industry due to its high performances. The PEMFC cannot certainly ensure a sustained required energy in some scenarios. To solve this problem related to PEMFC transient response, a Hybrid Electrical Storage System (HES) is a potential candidate for a solution. The proposed Hybrid Storage system is comprised of the battery (BT) and a Super-Capacitor (SC) components. These components are included to control the hydrogen variations and the fast peak powers scenarios respectively. The SC is used to control PEMFC and the BT slow dynamics at the same times. An accurate Multi-Ways Energy Management System (MW-EMS) is proposed which aims to cooperate with the system components through SC/BT state of charge and a flux calculation. The simulation results are discussed and assessed using MATLAB/ Simulink.
Indian Monuments Classification using Support Vector Machine
Authors: S. Bhatt Malay , P. Patalia Tejas 
Published: October 2017

Recently, Content-Based Image Retrieval is a widely popular and efficient searching and indexing approach used by knowledge seekers. Use of images by e-commerce sites, by product and by service industries is not new nowadays. Travel and tourism are the largest service industries in India. Every year people visit tourist places and upload pictures of their visit on social networking sites or share via the mobile device with friends and relatives. Classification of the monuments is helpful to hoteliers for the development of a new hotel with state of the art amenities, to travel service providers, to restaurant owners, to government agencies for security, etc.. The proposed system had extracted features and classified the Indian monuments visited by the tourists based on the linear Support Vector Machine (SVM). The proposed system was divided into 3 main phases: preprocessing, feature vector creation and classification. The extracted features are based on Local Binary Pattern, Histogram, Co-occurrence Matrix and Canny Edge Detection methods. Once the feature vector had been constructed, classification was performed using Linear SVM. The Database of 10 popular Indian monuments was generated with 50 images for each class. The proposed system is implemented in MATLAB and achieves very high accuracy. The proposed system was also tested on other popular benchmark databases.