bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seasonal patterns in Arctic prasinophytes and inferred ecology of Bathycoccus unveiled in an Arctic winter metagenome
Authors: Joli Nathalie , Monier Adam , Logares Ramiro , Lovejoy Connie 
Published: March 2017

Abstract
Prasinophytes occur in all oceans but rarely dominate phytoplankton populations. In contrast, a single ecotype of the prasinophyte Micromonas is frequently the most abundant photosynthetic taxon reported in the Arctic from summer through autumn. However, seasonal dynamics of prasinophytes outside of this period are little known. To address this, we analyzed high-throughput V4 18S rRNA amplicon data collected from November to July in the Amundsen Gulf Region, Beaufort Sea, Arctic. Surprisingly during polar sunset in November and December, we found a high proportion of reads from both DNA and RNA belonging to another prasinophyte, Bathycoccus. We then analyzed a metagenome from a December sample and the resulting Bathycoccus metagenome assembled genome (MAG) covered ~90% of the Bathycoccus Ban7 reference genome. In contrast, only ~20% of a reference Micromonas genome was found in the metagenome. Our phylogenetic analysis of marker genes placed the Arctic Bathycoccus in the B1 coastal clade. In addition, substitution rates of 129 coding DNA sequences were ~1.6% divergent between the Arctic MAG and coastal Chilean upwelling MAGs and 17.3% between it and a South East Atlantic open ocean MAG in the B2 Clade. The metagenomic analysis also revealed a winter viral community highly skewed toward viruses targeting Micromonas, with a much lower diversity of viruses targeting Bathycoccus. Overall a combination of Micromonas being relatively less able to maintain activity under dark winter conditions and viral suppression of Micromonas may have contributed to the success of Bathycoccus in the Amundsen Gulf during winter.
Steering surface topographies of electrospun fibers: understanding the mechanisms
Authors: Yazgan Gökçe , Dmitriev Ruslan I. , Tyagi Vasundhara , Jenkins James , Rotaru Gelu-Marius , Rottmar Markus , Rossi René M. , Toncelli Claudio , Papkovsky Dmitri B. , Maniura-Weber Katharina , Fortunato Giuseppino 
Published: March 2017

AbstractA profound understanding of how to tailor surface topographies of electrospun fibers is of great importance for surface sensitive applications including optical sensing, catalysis, drug delivery and tissue engineering. Hereby, a novel approach to comprehend the driving forces for fiber surface topography formation is introduced through inclusion of the dynamic solvent-polymer interaction during fiber formation. Thus, the interplay between polymer solubility as well as computed fiber jet surface temperature changes in function of time during solvent evaporation and the resultant phase separation behavior are studied. The correlation of experimental and theoretical results shows that the temperature difference between the polymer solution jet surface temperature and the dew point of the controlled electrospinning environment are the main influencing factors with respect to water condensation and thus phase separation leading to the final fiber surface topography. As polymer matrices with enhanced surface area are particularly appealing for sensing applications, we further functionalized our nanoporous fibrous membranes with a phosphorescent oxygen-sensitive dye. The hybrid membranes possess high brightness, stability in aqueous medium, linear response to oxygen and hence represent a promising scaffold for cell growth, contactless monitoring of oxygen and live fluorescence imaging in 3-D cell models.
G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity
Authors: Tatsumi Kasumi , Sakashita Gyosuke , Nariai Yuko , Okazaki Kosuke , Kato Hiroaki , Obayashi Eiji , Yoshida Hisashi , Sugiyama Kanako , Park Sam-Yong , Sekine Joji , Urano Takeshi 
Published: March 2017

AbstractThe recognition specificity of monoclonal antibodies (mAbs) has made mAbs among the most frequently used tools in both basic science research and in clinical diagnosis and therapies. Precise determination of the epitope allows the development of epitope tag systems to be used with recombinant proteins for various purposes. Here we describe a new family of tag derived from the epitope recognized by a highly specific mAb G196. The minimal epitope was identified as the five amino acid sequence Asp-Leu-Val-Pro-Arg. Permutation analysis was used to characterize the binding requirements of mAb G196, and the variable regions of the mAb G196 were identified and structurally analyzed by X-ray crystallography. Isothermal titration calorimetry revealed the high affinity (Kd = 1.25 nM) of the mAb G196/G196-epitope peptide interaction, and G196-tag was used to detect several recombinant cytosolic and nuclear proteins in human and yeast cells. mAb G196 is valuable for developing a new peptide tagging system for cell biology and biochemistry research.
Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity
Authors: Raciti Gregory A. , Spinelli Rosa , Desiderio Antonella , Longo Michele , Parrillo Luca , Nigro Cecilia , D’Esposito Vittoria , Mirra Paola , Fiory Francesca , Pilone Vincenzo , Forestieri Pietro , Formisano Pietro , Pastan Ira , Miele Claudia , Beguinot Francesco 
Published: March 2017

AbstractEpigenetic modifications alter transcriptional activity and contribute to the effects of environment on the individual risk of obesity and Type 2 Diabetes (T2D). Here, we have estimated the in vivo effect of a fat-enriched diet (HFD) on the expression and the epigenetic regulation of the Ankyrin repeat domain 26 (Ankrd26) gene, which is associated with the onset of these disorders. In visceral adipose tissue (VAT), HFD exposure determined a specific hyper-methylation of Ankrd26 promoter at the −436 and −431 bp CpG sites (CpGs) and impaired its expression. Methylation of these 2 CpGs impaired binding of the histone acetyltransferase/transcriptional coactivator p300 to this same region, causing hypo-acetylation of histone H4 at the Ankrd26 promoter and loss of binding of RNA Pol II at the Ankrd26 Transcription Start Site (TSS). In addition, HFD increased binding of DNA methyl-transferases (DNMTs) 3a and 3b and methyl-CpG-binding domain protein 2 (MBD2) to the Ankrd26 promoter. More importantly, Ankrd26 down-regulation enhanced secretion of pro-inflammatory mediators by 3T3-L1 adipocytes as well as in human sera. Thus, in mice, the exposure to HFD induces epigenetic silencing of the Ankrd26 gene, which contributes to the adipose tissue inflammatory secretion profile induced by high-fat regimens.
Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos
Authors: Fini Jean-Baptiste , Mughal Bilal B. , Le Mével Sébastien , Leemans Michelle , Lettmann Mélodie , Spirhanzlova Petra , Affaticati Pierre , Jenett Arnim , Demeneix Barbara A. 
Published: March 2017

AbstractThyroid hormones are essential for normal brain development in vertebrates. In humans, abnormal maternal thyroid hormone levels during early pregnancy are associated with decreased offspring IQ and modified brain structure. As numerous environmental chemicals disrupt thyroid hormone signalling, we questioned whether exposure to ubiquitous chemicals affects thyroid hormone responses during early neurogenesis. We established a mixture of 15 common chemicals at concentrations reported in human amniotic fluid. An in vivo larval reporter (GFP) assay served to determine integrated thyroid hormone transcriptional responses. Dose-dependent effects of short-term (72 h) exposure to single chemicals and the mixture were found. qPCR on dissected brains showed significant changes in thyroid hormone-related genes including receptors, deiodinases and neural differentiation markers. Further, exposure to mixture also modified neural proliferation as well as neuron and oligodendrocyte size. Finally, exposed tadpoles showed behavioural responses with dose-dependent reductions in mobility. In conclusion, exposure to a mixture of ubiquitous chemicals at concentrations found in human amniotic fluid affect thyroid hormone-dependent transcription, gene expression, brain development and behaviour in early embryogenesis. As thyroid hormone signalling is strongly conserved across vertebrates the results suggest that ubiquitous chemical mixtures could be exerting adverse effects on foetal human brain development.
Renal systolic time intervals derived from intra-renal artery Doppler as a novel predictor of adverse cardiac outcomes
Authors: Lee Wen-Hsien , Hsu Po-Chao , Chu Chun-Yuan , Chen Szu-Chia , Lee Hung-Hao , Lee Meng-Kuang , Lee Chee-Siong , Yen Hsueh-Wei , Lin Tsung-Hsien , Voon Wen-Chol , Lai Wen-Ter , Sheu Sheng-Hsiung , Kuo Po-Lin , Su Ho-Ming 
Published: March 2017

AbstractThe aim of this study was to evaluate the use of renal systolic time intervals measured by electrocardiographic gated Doppler ultrasonography for predicting adverse cardiac events. This longitudinal observation study enrolled 205 patients. Renal systolic time intervals, including pre-ejection period (PEP) and ejection time (ET), and ratio of renal PEP to ET, were measured by electrocardiographic gated Doppler ultrasound. The 14 adverse cardiac events identified in this population included 9 cardiac deaths and 5 hospitalizations for heart failure during an average follow up of 30.9 months (25th–75th percentile: 30–33 months). Renal PEP (hazard ratio = 1.023, P = 0.001), renal ET (hazard ratio = 0.975, P = 0.001) and renal PEP/ET (per 0.01 unit increase, hazard ratio = 1.060, P < 0.001) were associated with poor cardiac outcomes. The addition of renal PEP/ET to a Cox model containing important clinical variables and renal resistive index further improved the value in predicting adverse cardiac events (Chi-square increase, 9.996; P = 0.002). This study showed that parameters of intra-renal hemodynamics were potential predictors of adverse cardiac outcomes. However, the generalizability of these indicators need to be investigated in future large-scale studies.
Confocal imaging of mouse mandibular condyle cartilage
Authors: He Y. , Zhang M. , Huang A. Y. , Cui Y. , Bai D. , Warman M. L. 
Published: March 2017

AbstractMice are commonly used to study the temporomandibular joint (TMJ) and to model human TMJ disease. However, evaluating TMJ pathology in mice using standard histologic methods is time consuming, labor intensive, and dependent upon investigators’ expertise at consistently orienting and sectioning across tiny specimens. We describe a method that uses confocal microscopy to rapidly and reliably assess indicators of mandibular condyle cartilage pathology in mice. We demonstrate the utility of this method for detecting abnormalities in chondrocyte distribution in mice lacking lubricin (Prg4), the major boundary lubricant of articular cartilage. We further show that the method can provide information about recombination sites and efficiency in mandibular cartilage for Cre-driver strains. Because specimen preparation and data acquisition with confocal microscopy are simple and fast, the method can serve as a primary screening tool for TMJ pathology, before proceeding to complicated, time consuming, secondary analyses.
Targeted antigen delivery to dendritic cells elicits robust antiviral T cell-mediated immunity in the liver
Authors: Volckmar Julia , Gereke Marcus , Ebensen Thomas , Riese Peggy , Philipsen Lars , Lienenklaus Stefan , Wohlleber Dirk , Klopfleisch Robert , Stegemann-Koniszewski Sabine , Müller Andreas J. , Gruber Achim D. , Knolle Percy , Guzman Carlos A. , Bruder Dunja 
Published: March 2017

AbstractHepatotropic viruses such as hepatitis C virus cause life-threatening chronic liver infections in millions of people worldwide. Targetedin vivoantigen-delivery to cross-presenting dendritic cells (DCs) has proven to be extraordinarily efficient in stimulating antigen-specific T cell responses. To determine whether this approach would as well be suitable to induce local antiviral effector T cells in the liver we compared different vaccine formulations based on either the targeting of DEC-205 or TLR2/6 on cross-presenting DCs or formulations not involvingin vivoDC targeting. As read-outs we usedin vivohepatotropic adenovirus challenge, histology and automated multidimensional fluorescence microscopy (MELC). We show that targetedin vivoantigen delivery to cross-presenting DCs is highly effective in inducing antiviral CTLs capable of eliminating virus-infected hepatocytes, while control vaccine formulation not involving DC targeting failed to induce immunity against hepatotropic virus. Moreover, we observed distinct patterns of CD8+T cell interaction with virus-infected and apoptotic hepatocytes in the two DC-targeting groups suggesting that the different vaccine formulations may stimulate distinct types of effector functions. Our findings represent an important step toward the future development of vaccines against hepatotropic viruses and the treatment of patients with hepatic virus infection after liver transplantation to avoid reinfection.
Tree-ring width reveals the preparation of the 1974 Mt. Etna eruption
Authors: Seiler Ruedi , Houlié Nicolas , Cherubini Paolo 
Published: March 2017

AbstractReduced near-infrared reflectance observed in September 1973 in Skylab images of the western flank of Mt. Etna has been interpreted as an eruption precursor of the January 1974 eruption. Until now, it has been unclear when this signal started, whether it was sustained and which process(es) could have caused it. By analyzing tree-ring width time-series, we show that the reduced near-infrared precursory signal cannot be linked to a reduction in annual tree growth in the area. However, comparing the tree-ring width time-series with both remote sensing observations and volcano-seismic activity enables us to discuss the starting date of the pre-eruptive period of the 1974 eruption.
Ultralong well-aligned TiO2:Ln3+ (Ln = Eu, Sm, or Er) fibres prepared by modified electrospinning and their temperature-dependent luminescence
Authors: Yu Hongquan , Li Yue , Song Yang , Wu Yanbo , Lan Xijie , Liu Shimin , Tang Yanning , Xu Shasha , Chen Baojiu 
Published: March 2017

AbstractElectrospinning has emerged as an attractive technique for the fabrication of ultrafine fibres in micro-/nano-scale fineness: however, it remains a significant technological challenge to assemble aligned fibre arrays via an conventional electrospinning method due to the inherent whipping instability of the polymeric jet. We herein have first developed a simple modified electrospinning method with which to prepare ultralong (>300 mm) well-aligned inorganic fibre arrays, i.e., using an ultrahigh molecular weight polymer to suppress or eliminate the whipping motion of the electrospun jet, has emerged as a facile approach for the continuous fabrication of well-aligned, ultralong fibres through simply using a rotating cylinder as the collector (it was not found necessary to use a very high rotating speed, extra magnetic, electrical field) in the electrospinning process. As result, the ultralong well-aligned TiO2:Ln3+ (Ln = Eu, Sm, or Er) fibre arrays can be obtained from ultrahigh molecular weight poly(ethylene oxide), tetra-n-butyl titanate (Ti(OC4H9)4) and lanthanide nitrate in the modified electrospinning approach. The grow mechanism and luminescent properties of these ultralong well-aligned TiO2:Ln3+ fibre arrays were also investigated.
Ablation of neuropsin–neuregulin 1 signaling imbalances ErbB4 inhibitory networks and disrupts hippocampal gamma oscillation
Authors: Kawata M , Morikawa S , Shiosaka S , Tamura H 
Published: March 2017

AbstractParvalbumin-expressing interneurons are pivotal for the processing of information in healthy brain, whereas the coordination of these functions is seriously disrupted in diseased brain. How these interneurons in the hippocampus participate in pathological functions remains unclear. We previously reported that neuregulin 1 (NRG1)–ErbB4 signaling, which is actuated by neuropsin, is important for coordinating brain plasticity. Neuropsin cleaves mature NRG1 (bound to extracellular glycosaminoglycans) in response to long-term potentiation or depression, liberating a soluble ligand that activates its receptor, ErbB4. Here, we show in mice that kainate-induced status epilepticus transiently elevates the proteolytic activity of neuropsin and stimulates cFos expression with a time course suggesting that activation of ErbB4- and parvalbumin-expressing interneurons follows the excitation and subsequent silencing of pyramidal neurons. In neuropsin-deficient mice, kainate administration impaired signaling and disrupted the neuronal excitation–inhibition balance (E/I balance) in hippocampal networks, by decreasing the activity of parvalbumin-positive interneurons while increasing that of pyramidal neurons, resulting in the progression of status epilepticus. Slow, but not fast, gamma oscillations in neuropsin-deficient mice showed reduced power. Intracerebroventricular infusion of the soluble NRG1 ligand moiety restored the E/I balance, status epilepticus and gamma oscillations to normal levels. These results suggest that the neuropsin–NRG1 signaling system has a role in pathological processes underlying temporal lobe epilepsy by regulating the activity of parvalbumin-expressing interneurons, and that neuropsin regulates E/I balance and gamma oscillations through NRG1–ErbB4 signaling toward parvalbumin-expressing interneurons. This neuronal system may be a useful target of pharmacological therapies against cognitive disorders.
Perspective on precision medicine in paediatric heart failure
Authors: Fridman Michael D. , Mital Seema 
Published: March 2017

In 2015, President Obama launched the Precision Medicine Initiative (PMI), which introduced new funding to a method of research with the potential to study rare and complex diseases. Paediatric heart failure, a heterogeneous syndrome affecting approximately 1 in 100000 children, is one such condition in which precision medicine techniques may be applied with great benefit. Current heart failure therapies target downstream effects of heart failure rather than the underlying cause of heart failure. As such, they are often ineffective in paediatric heart failure, which is typically of primary (e.g. genetic) rather than secondary (e.g. acquired) aetiology. It is, therefore, important to develop therapies that can target the causes of heart failure in children with greater specificity thereby decreasing morbidity, mortality and burden of illness on both patients and their families. The benefits of co-ordinated research in genomics, proteomics, metabolomics, transcriptomics and phenomics along with dietary, lifestyle and social factors have led to novel therapeutic and prognostic applications in other fields such as oncology. Applying such co-ordinated research efforts to heart failure constitutes an important step in advancing care and improving the lives of those affected.
A Cross-Language Study of Laryngeal-Oral Coordination Across Varying Prosodic and Syllable-Structure Conditions
Authors: Hoole Philip , Bombien Lasse 
Published: March 2017

Purpose
The purpose of this study is to use prosodic and syllable-structure variation to probe the underlying representation of laryngeal kinematics in languages traditionally considered to differ in voicing typology (German vs. Dutch and French).

Method
Transillumination and videofiberendoscopic filming were used to investigate the devoicing gesture in German, Dutch, and French for material that compared, first, a strong versus weak prosodic condition and, second, singletons versus clusters (stop + /r/ and /l/).

Results
The results showed strengthening of the devoicing gesture in the strong prosodic condition and in the segmental context stop + /r/ for German and French but not for Dutch. In terms of timing (duration of oral occlusion, voice onset time, timing of peak glottal opening relative to stop release), French was intermediate between German and Dutch.

Conclusions
(a) The representation of French voiceless plosives requires an active specification for glottal spreading just as in German. (b) Static features are not well suited to capturing cross-language differences in voicing typology and changes in voicing specification over time.
Severe Form of Brachydactyly Type A1 in a Child with a c.298G > A Mutation in IHH Gene
Authors: Salian Smrithi , Shukla Anju , Nishimura Gen , Girisha Katta 
Published: March 2017

AbstractBrachydactyly type A1 (BDA1) is characterized by short middle phalanges. We report the case of a child with a severe form of BDA1 with complete absence of the middle phalanges of all extremities. He had c.298G > A (p.D100N) mutation in IHH gene.
Sensorineural Hearing Loss in a Patient Affected by Congenital Cytomegalovirus Infection: Is It Useful to Identify Comorbid Pathologies?
Authors: Melis D. , D'Amico A. , Cappuccio G. , Auletta G. , Vassallo P. , Genesio R. , Nitsch L. , Buffolano W. , Fontana P. 
Published: March 2017

AbstractSensorineural hearing loss (SNHL) is a common defect with a multifactorial etiology. Congenital cytomegalovirus infection (cCMV) is the most common infectious cause, and its early detection allows a prompt pharmacological treatment that can improve hearing prognosis. In a consistent percentage of profound SNHL, genetic causes and/or inner ear malformations are involved; their prompt diagnosis might change therapeutic options. This study reports a case of a 3- year-old female patient with symptomatic cCMV infection who also exhibits developmental delay, dysmorphic facial features, bilateral hearing loss, and cochlear incomplete partition, type 2, in 7q21.3 deletion. This deletion includes the genes DLX5 and DLX6, which could be the candidate genes for the ear malformation named incomplete partition, type 2.
Design, Network Analysis and In Silico Modeling of Biologically Significant 4-(substituted benzyl)-2-Amino-6-HydroxyPyrimidine-5-Carboxamide Nanoparticles
Authors: Panneerselvam Theivendren , Sivakumar Vellaichamy , Arumugam Subramanian , Selvaraj Kunjiappan , Indhumathy Murugan 
Published: March 2017

Abstract
The synthesized 4-(4-hydroxy benzyl)-2-amino-6-hydroxy pyrimidine-5-carboxamide was chosen to perform in silico modeling with identified drug target AGT, TNF, F2 and BCL2L1. The identified human proteins are vital in the pain management and also an important target for the study of wound healing activity.
The enzymes were identified by using BioGRID, string database and network analysis through Cytoscape software. The wound healing activity was evaluated by excision wound model.
The observed results revealed that, the pyrimidine nanoparticles showed significant wound healing activity compared to standard and synthesized compound. The detailed synthesis of nanoparticles formulation spectral analysis and pharmacological screening data’s were reported.
The revealed reports of synthesized analogues and formulated nanoparticles will generate a very good impact to the chemists and research scholars for further investigations in wound healing and pain management.