bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface charge modification decreases Pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model
Authors: Kao W. Katherine , Gagnon Patricia M. , Vogel Joseph P. , Chole Richard A. 
Published: March 2017

ObjectiveChronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. These infections are thought to be due to the establishment of microbial biofilms on implant surfaces. To address this issue, we hypothesized that surface charge modification may inhibit the formation of Pseudomonas aeruginosa biofilms on implant surfaces in vitro and in vivo.Study DesignWe evaluated the effect of surface charge modification on bacterial biofilm formation by assessing the effect of the surface charge on bacterial adhesion in vitro and bacterial persistence in vivo.MethodsTo study the effect of surface charge in vitro, the surface wells in culture plates were modified using a layer‐by‐layer polyelectrolyte assembly method. Bacterial adherence was measured at 30‐, 60‐, and 120‐minute intervals. To study the effect of surface charge modification in vivo, the surface of titanium microscrews was similarly modified and then surgically implanted into the dorsal calvaria of adult rats and inoculated with bacteria. Two weeks after implantation and inoculation, the number of bacteria remaining in vivo was evaluated.ResultsSurface charge modification results in a significant decrease in adherence of bacteria in vitro. Surface charge modification of titanium microscrew implants also resulted in a significant decrease in P. aeruginosa recovered 2 weeks after surgical implantation.ConclusionCharge modification decreases the number of bacteria adherent to a surface in vitro and decreases the risk and severity of implant infection in an in vivo rat infection model. These results have promising biomedical applications.Level of EvidenceNA. Laryngoscope, 127:1655–1661, 2017
Modified transnasal endoscopic medial maxillectomy through prelacrimal duct approach
Authors: Suzuki Motohiko , Nakamura Yoshihisa , Yokota Makoto , Ozaki Shinya , Murakami Shingo 
Published: March 2017

ObjectiveWe previously reported a modified endoscopic medial maxillectomy (modified transnasal endoscopic medial maxillectomy through prelacrimal duct approach [MTEMMPDA]) to resect inverted papilloma (IP), for which the inferior turbinate (IT) and nasolacrimal duct (ND) can be preserved. MTEMMPDA is a safe and effective method to obtain wide, straight access to the maxillary sinus (MS). However, there are few reported cases of patients who underwent MTEMMPDA, and even fewer of patients who underwent partial osteotomy of the apertura piriformis and the anterior wall of the MS. In this study, we analyzed the outcomes of 51 patients who underwent MTEMMPDA.Study DesignRetrospective review.MethodsAll patients who underwent MTEMMPDA at our hospital between January 2004 and December 2015 were included in this study.ResultsFifty‐one patients with sinonasal IP in the MS underwent MTEMMPDA. Recurrence was seen in the MS of one patient (follow‐up of 2–138 months). The IT remained unchanged in all 51 patients without atrophy. We have not observed epiphora, eye discharge, dry nose, or persistent crusting after this surgery. Although seven patients had numbness around the upper lip after surgery, this had disappeared by 1 year after surgery. Additional partial osteotomy of the apertura piriformis and the anterior wall of the MS were done in eight patients. Deformation of the external nose was not seen.ConclusionThis approach appears to be a safe and effective method to resect IP in the MS, even if there is additional partial osteotomy of the apertura piriformis and the anterior wall of the MS.Level of Evidence4.Laryngoscope, 127:2205–2209, 2017
Staging cutaneous squamous cell carcinoma metastases to the parotid gland
Authors: Czerwonka Lukasz , De Santis Robert J. , Horowitz Gilad , Hong Michael , Orsini Mario , Enepekides Danny , Goldstein David P. , Dort Joe , Higgins Kevin 
Published: March 2017

ObjectiveThe staging of cutaneous squamous cell cancers (cSCC) was revised by the American Joint Committee on Cancer in 2010 to incorporate known prognostic factors and expand the N (node) category. The purpose of this study was to validate this staging system using a North American cohort, and to compare it to the O'Brien P (Parotid) and N staging system.MethodsAn exhaustive collaborative database search was performed for all patients with cSCC metastasis to the parotid gland treated at three major Canadian tertiary referral centers from December 1999 to March 2015. The data collected for each patient included overall survival; disease‐free survival; tumor, node, and metastasis) staging; and postoperative radiation status. Post‐hoc analysis was completed to discern the strongest prognostic factors of survival as they relate to the abovementioned staging systems.ResultsOf 136 patients identified, 80% had a documented history of previously treated head and neck cSCC an average of 27 months prior to presentation. Average size of the parotid lesion at recurrence was 4.5 cm. Ninety‐six percent of patients underwent surgical resection of the parotid metastasis. Five‐year overall and disease‐free survival is 79% and 55%, respectively. Only cSCC staging and cSCC‐N category had statistically significant differences between groups. cSCC staging had the largest percentage of variation in overall survival explained.ConclusionPatients with cSCC metastasis to the parotid gland proved to have a moderate survival rate, despite presenting with advanced disease. cSCC staging in the setting of parotid metastasis, despite its limitations, currently offers the most predictive staging system available.Level of Evidence4. Laryngoscope, 127:2063–2069, 2017
Malignant glomus tumors of the head and neck in children and adults: Evaluation and management
Authors: Wolter Nikolaus E. , Adil Eelam , Irace Alexandria L. , Werger Annette , Perez‐Atayde Antonio R. , Weldon Christopher , Orbach Darren B. , Rodriguez‐Galindo Carlos , Rahbar Reza 
Published: March 2017

Objectives/HypothesisTo describe our current multidisciplinary approach to pediatric malignant glomus tumors of the head and neck and review the current literature.Study DesignRetrospective chart review at a tertiary referral children's hospital and a comprehensive literature review.MethodsA comprehensive literature search of PubMed, Embase, Web of Science, Google Scholar, and EBSCO with respect to malignant glomus tumors of the head and neck was conducted. We obtained expert input from other pertinent specialties, including oncology, pathology, and radiology. To highlight the difficulty of evaluation and management of these patients, we also present a pediatric patient with a left neck malignant glomus tumor and lung metastases.ResultsOnly two cases of pediatric malignant glomus tumor (including our own) have been reported in the English literature. Overall, 14 malignant glomus tumors have been reported in the head and neck (11 primary and three metastatic). Surgical resection is the mainstay of treatment, but local recurrence is common (five of 11, 45%).ConclusionsMalignant glomus tumor of the head and neck is an extremely rare tumor in children. Evaluation consists of imaging, and tissue biopsy is necessary for definitive diagnosis. Management options include surgical resection with or without an adjuvant chemotherapy protocol similar to those designed for sarcoma. Additional reports are necessary so that we may determine the utility, if any, of radiotherapy in the management of this tumor. Laryngoscope, 127:2873–2882, 2017
Combined XIL‐6R and urocortin‐2 treatment restores MDX diaphragm muscle force
Authors: Manning Jennifer , Buckley Maria M. , O'Halloran Ken D. , O'Malley Dervla 
Published: March 2017

ABSTRACTIntroduction: Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration leading to immobility, respiratory failure, and premature death. As chronic inflammation and stress are implicated in DMD pathology, the efficacy of an anti‐inflammatory and anti‐stress intervention strategy in ameliorating diaphragm dysfunction was investigated. Methods: Diaphragm muscle contractile function was compared in wild‐type and dystrophin‐deficient mdx mice treated with saline, anti‐interleukin‐6 receptor antibodies (xIL‐6R), the corticotrophin‐releasing factor receptor 2 (CRFR2) agonist, urocortin 2, or both xIL‐6R and urocortin 2. Results: Combined treatment with xIL‐6R and urocortin 2 rescued impaired force in mdx diaphragms. Mechanical work production and muscle shortening was also improved by combined drug treatment. Discussion: Treatment which neutralizes peripheral IL‐6 signaling and stimulates CRFR2 recovers force‐generating capacity and the ability to perform mechanical work in mdx diaphragm muscle. These findings may be important in the search for therapeutic targets in DMD. Muscle Nerve 56: E134–E140, 2017
Genome‐wide analysis identifies an african‐specific variant in SEMA4D associated with body mass index
Authors: Chen Guanjie , Doumatey Ayo P. , Zhou Jie , Lei Lin , Bentley Amy R. , Tekola-Ayele Fasil , Adebamowo Sally N. , Baker Jennifer L. , Fasanmade Olufemi , Okafor Godfrey , Eghan Benjamin , Agyenim‐Boateng Kofi , Amoah Albert , Adebamowo Clement , Acheampong Joseph , Johnson Thomas , Oli Johnnie , Shriner Daniel , Adeyemo Adebowale A. , Rotimi Charles N. 
Published: March 2017

ObjectiveThe prevalence of obesity varies between ethnic groups. No genome‐wide association study (GWAS) for body mass index (BMI) has been conducted in continental Africans.MethodsWe performed a GWAS for BMI in 1,570 West Africans (WA). Replication was conducted in independent samples of WA (n = 1,411) and African Americans (AA) (n = 9,020).ResultsWe identified a novel genome‐wide significant African‐specific locus for BMI (SEMA4D, rs80068415; minor allele frequency = 0.008, P = 2.10 × 10−8). This finding was replicated in independent samples of WA (P = 0.013) and AA (P = 0.017). Individuals with obesity had higher serum SEMA4D levels compared to those without obesity (P < 0.0001), and elevated levels of serum SEMA4D were associated with increased obesity risk (OR = 4.2, P < 1 × 10−4). The prevalence of obesity was higher in individuals with the CT versus TT genotypes (55.6% vs. 22.9%).ConclusionsA novel variant in SEMA4D was significantly associated with BMI. Carriers of the C allele were 4.6 BMI units heavier than carriers of the T allele (P = 0.0007). This variant is monomorphic in Europeans and Asians, highlighting the importance of studying diverse populations. While there is evidence for the involvement of SEMA4D in inflammatory processes, this study is the first to implicate SEMA4D in obesity pathophysiology.
Epithelial membrane protein 2 (EMP2) deficiency alters placental angiogenesis, mimicking features of human placental insufficiency
Authors: Williams Carmen J , Chu Alison , Jefferson Wendy N , Casero David , Sudhakar Deepthi , Khurana Nevil , Hogue Claire P , Aryasomayajula Chinmayi , Patel Priya , Sullivan Peggy , Padilla‐Banks Elizabeth , Mohandessi Shabnam , Janzen Carla , Wadehra Madhuri 
Published: March 2017

AbstractEpithelial membrane protein‐2 (EMP2) is a tetraspan protein predicted to regulate placental development. Highly expressed in secretory endometrium and trophectoderm cells, previous studies suggest that it may regulate implantation by orchestrating the surface expression of integrins and other membrane proteins. In order to test the role of EMP2 in pregnancy, mice lacking EMP2 (Emp2−/−) were generated. Emp2−/− females are fertile but have reduced litter sizes when carrying Emp2−/− but not Emp2+/− fetuses. Placentas of Emp2−/− fetuses exhibit dysregulation in pathways related to neoangiogenesis, coagulation, and oxidative stress, and have increased fibrin deposition and altered vasculature. Given that these findings often occur due to placental insufficiency resulting in an oxygen‐poor environment, the expression of hypoxia‐inducible factor‐1 alpha (HIF‐1α) was examined. Placentas from Emp2−/− fetuses had increased total HIF‐1α expression in large part through an increase in uterine NK (uNK) cells, demonstrating a unique interplay between uNK cells and trophoblasts modulated through EMP2. To determine if these results translated to human pregnancy, placentas from normal, term deliveries or those complicated by placental insufficiency resulting in intrauterine growth restriction (IUGR) were stained for EMP2. EMP2 was significantly reduced in both villous and extravillous trophoblast populations in IUGR placentas. Experiments in vitro using human trophoblast cells lines indicate that EMP2 modulates angiogenesis by altering HIF‐1α expression. Our results reveal a novel role for EMP2 in regulating trophoblast function and vascular development in mice and humans, and suggest that it may be a new biomarker for placental insufficiency. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The effect of dimethylsulfoxide on the dissociation process of physical complexes of polyacrylonitrile in N,N‐dimethylformamide
Authors: Eom Youngho , Kim Byoung Chul 
Published: March 2017

AbstractThe physical properties and gelation behavior of polyacrylonitrile (PAN) solutions were investigated in mixed solvents of N,N‐dimethylformamide (DMF) and dimethylsulfoxide (DMSO). For the individual solubility parameters, DMSO had the polar term closer to PAN than DMF. Small‐angle X‐ray scattering profiles and Fourier transform infrared spectra of 20 wt% PAN solutions confirmed that the internal structure and intermolecular complexes between nitrile groups were broken up by the DMSO molecules. The slope of Cole–Cole plot, a measure of the solution heterogeneity, increased with the mole fraction of DMSO (XDMSO) via three distinct zones. The homogenization was particularly noticeable in Zone 1 (0.0 < XDMSO < 0.4) and Zone 3 (0.7 < XDMSO < 1.0). The Huggins constant and UV‐visible absorption at 268 nm of dilute solutions decreased sharply above XDMSO of 0.7 in Zone 3. This indicated a marked increase of PAN solubility by dissociating the intramolecular complexes between nitrile groups. In Zone 1, the storage modulus of 20 wt% PAN solutions decreased but loss modulus remained almost constant with XDMSO at lower frequency below 1 rad s−1, indicating the weakening of the true entanglement points of the intermolecular complexes. The mechanism indicated that the intermolecular complexes between nitrile groups were dissociated by DMSO earlier than the intramolecular ones. © 2017 Society of Chemical Industry
Kinetic energy density for orbital‐free density functional calculations by axiomatic approach
Authors: Alharbi Fahhad H. , Kais Sabre 
Published: March 2017

AbstractAn axiomatic approach is herein used to determine the physically acceptable forms for general D‐dimensional kinetic energy density functionals (KEDF). The resulted expansion captures most of the known forms of one‐point KEDFs. By statistically training the KEDF forms on a model problem of noninteracting kinetic energy in 1D (six terms only), the mean relative accuracy for 1000 randomly generated potentials is found to be better than the standard KEDF by several orders of magnitudes. The accuracy improves with the number of occupied states and was found to be better than
for a system with four occupied states. Furthermore, we show that free fitting of the coefficients associated with known KEDFs approaches the exactly analytic values. The presented approach can open a new route to search for physically acceptable kinetic energy density functionals and provide an essential step toward more accurate large‐scale orbital free density functional theory calculations.
A Pyrene‐Pyrazole‐Based Rotamer Senses Hg2+ on the Nanomolar Scale
Authors: Naskar Barnali , Dhara Anamika , Modak Ritwik , Maiti Dilip K. , Prodhan Chandraday , Chaudhuri Keya , Requena Alberto , Cerón‐Carrasco José Pedro , Goswami Sanchita 
Published: March 2017

AbstractIn this experimental and theoretical study, a new pyrene‐pyrazole based chemosensor, 5‐Methyl‐1H‐pyrazole‐3‐carboxylic acid pyren‐1‐ylm ethylene hydrazide (MpcphH), is designed and prepared for selective sensing of Hg2+. Photophysical studies, including UV‐vis and fluorescence, showed that MpcphH exhibited high selectivity and sensitivity in response to Hg2+ with detection limit in the nano molar range. The 1H and 13C−NMR spectroscopic techniques are presented to elucidate the coordination modes of MpcphH towards Hg2+. The relative stability of the ketoimine/imineol tautomers and the specific rotamer responsible for Hg2+ coordination are evaluated by means of DFT calculations. Furthermore, MpcphH provides a reliable approach to monitor cellular cytoplasmic Hg2+ in HepG2 cells.
Hydrothermal Synthesis of CuFe2O4 Magnetic Nanoparticles as Active and Robust Catalyst for N‐arylation of Indole and Imidazole with Aryl Halide
Authors: Nakhate Akhil V. , Yadav Ganapati D. 
Published: March 2017

AbstractSpinel ferrite magnetic nanoparticles are superior to the conventional catalysts. Here we report the synthesis of different types of spinel ferrite nanoparticles, MFe2O4 (M= Cu, Mn, Mg, Co, Ni), by a hydrothermal method. The prepared spinel ferrite nanoparticles were used for the N‐arylation reaction of indole with iodobenzene. Among these CuFe2O4 nanoparticles exhibited the best activity and selectivity for N‐arylation under ligand free condition. The prepared spinel ferrite microspheres were well characterised by using various characterization techniques such as TEM, XRD, XPS, SEM, EDXA, FT‐IR, N2 adsorption desorption measurements and TGA. The effect of various parameters were studied such as solvent, base, speed of agitation, mole ratio, catalyst loading and temperature in the reaction of indole with iodobenzene. Best conversion and yield were achieved at 110 °C and 1×10‐3 g/cm3 catalyst loading. Notably, CuFe2O4 magnetic nanoparticles were completely recovered and could be reused six times without any noticeable loss of activity. Reaction mechanism and kinetics are also presented.
Efficient CoMoS Catalysts Supported on Bio‐Inspired Polymer Coated Alumina for Hydrotreating Reactions
Authors: Munirathinam Rajesh , Laurenti Dorothée , Pirngruber Gerhard D. , Uzio Denis 
Published: March 2017

AbstractOrganic additives are widely used in order to improve the performance of hydrotreating catalysts. Yet, the use of polymers, in particular bio‐polymers, is less widespread. We present, herein, a strategy to coat alumina support with a bio‐polymer, namely polydopamine (Pdop). The Pdop coated supports provide a higher number of adsorption sites for Mo species compared to the bare support. The hydrotreating catalyst prepared on Pdop coated support showed improved performances for various hydrotreating reactions (aromatic hydrogenation, gas‐oil HDS and HDN). The increase in activity is ascribed to a better accessibility of active sites, and the presence of carbon adlayer between the active phase and alumina limiting detrimental direct metal support interactions. Modification of the alumina surfaces by Pdop creates new adsorption sites for metallic precursors, thereby allowing an improved accessibility of active sites especially at high surface densities.
Keggin Type‐Polyoxometalate Decorated Ruthenium Nanoparticles: Highly Active and Selective Nanocatalyst for the Oxidation of Veratryl Alcohol as a Lignin Model Compound
Authors: Baguc Ismail Burak , Saglam Serif , Ertas Ilknur Efecan , Keles Muhammed Nuri , Celebi Metin , Kaya Murat , Zahmakiran Mehmet 
Published: March 2017

AbstractDescribed herein is a new nanocatalyst system that efficiently works in the aerobic oxidation of veratryl alcohol (VA), which is formed by cleavage of β‐O‐4 linkages in lignin, to veratraldehyde (VAL) under mild reaction conditions. The new nanocatalyst system comprised of ruthenium(0) nanoparticles supported on the Keggin type polyoxometalate (POM; K3[PMo12O40]) network (Ru/POM) can simply and reproducibly be prepared by the dimethylamine‐borane ((CH3)2NHBH3) reduction of ruthenium(III) chloride trihydrate (RuCl3.3H2O) in isopropanol solution of K3[P Mo12O40] at room temperature. The characterization of Ru/POM by the combination of various analytical techniques reveals that the formation of well‐dispersed ruthenium(0) nanoparticles with a mean diameter of 4.7 ± 1.2 nm on the surface of POM network structure. This new Ru/POM nanocatalyst displays remarkable activity (TOF=7.5 mol VAld/mol Ru×h) at high selectivity (> 98%) and almost complete conversion (98%) in the aerobic oxidation of VA to VAld under mild conditions.
Rh(III)‐Catalyzed Synthesis of Multisubstituted Isoquinolines from Benzylamines and Diazo Compounds
Authors: Lin Xing , Chauvin Remi , Yu Yunliang , Wang Lianhui , Xu Linhua , Cui Xiuling 
Published: March 2017

AbstractMultisubstituted isoquinolines have been prepared by Rh(III)‐catalyzed cyclization of benzylamines with diazo compounds via aryl C−H bond activation using molecular oxygen from air as an oxidant. This method features mild reaction conditions, simple catalytic system, tolerance of a broad range of substrates, high efficiency and regioselectivity.
Furfuryl Alcohol and Furfural Hydrogenation over Activated Carbon–supported Palladium Catalyst in Presence of Water and Carbon Dioxide
Authors: Nanao Hidetaka , Murakami Yuka , Sato Osamu , Yamaguchi Aritomo , Hiyoshi Norihito , Shirai Masayuki 
Published: March 2017

AbstractHydrogenation of furfuryl alcohol and furfural over an activated carbon‐supported palladium catalyst was carried out and the effects of solvent and carbon dioxide on the activity and selectivity of the reactions were studied. For the hydrogenation of furfuryl alcohol and furfural, the addition of protic solvents led to a higher initial activity compared to that under neat conditions. Among the protic solvents, water caused the highest increase in the initial activities. When carbon dioxide was added to protic solvents, the initial activities increased further. For a system containing both water and carbon dioxide, the initial activity increased when the pressure of carbon dioxide introduced was increased up to 10 MPa, after which the activity remained almost constant. The results of the hydrogenation of furfural over the supported palladium catalyst showed that tetrahydrofurfural and furfuryl alcohol were the initial products; furfuryl alcohol was subsequently reduced to tetrahydrofurfuryl alcohol.