bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revealing the Molecular Signatures of miR-185-5p on Breast Cancer Cells Using Proteomic Analysis
Authors: Torun Vildan , Degerli Elif , Cansaran-Duman Demet 
Published: September 2024

Background:
Breast cancer is a heterogeneous type of disease in which genetic and environmental
factors play a crucial role. There are several types of treatment for breast cancer (BC)
patients. However, the biggest problem in the treatment of breast cancer is the resistance that occurs
during the treatment with chemotherapeutic agents. Usnic acid, a secondary metabolite of
lichen, has been identified as a drug candidate molecule in cancer treatment. The determination of
miRNA target proteins is essential for the understanding of molecular mechanisms of miRNA-related
tumorigenesis.

Objectives:
We determined that mir-185-5p has therapeutic potential at the miRNA level by applying
usnic acid to BT-474 breast cancer cells in a previous study. Herein, we aimed to investigate
the molecular mechanisms of miR-185-5p on BT-474 breast cancer cells using a proteomics approach.
We explored the changes in the protein expression level of BT-474 breast cancer cells in
response to the up-regulation of miR-185-5p after applying usnic acid as a novel candidate anti--
cancer drug molecule.

Methods:
We performed quantitative proteome analysis based on an LC-MS/MS assay, which
was validated by western blotting. The differentially expressed proteins were analyzed using the
latest data available in bioinformatics tools. The up-regulated expression of YWHAE, Cathepsin
D, and the down-regulated levels of PAK-1 were demonstrated by western blot assay.

Results:
According to the results, 86 proteins showing >2-fold change were identified as differentially
expressed between breast cancer and normal breast epithelial cells. The apoptosis pathway
was the main clade containing most of the proteins regulated by miR-185-5p. The results indicate
that miR-185-5p modulates apoptosis signaling pathways in BT-474 breast cancer cells. Breast
cancer inhibition due to increased expression of YWHAE, Cathepsin D, and decreased expression
of PAK-1 is likely to be mediated by inducing miR-185-5p mediated apoptosis.

Conclusion:
In this study, the identification of miR-185-5p protein targets demonstrated the potential
for the development of targeted therapy and the development of miRNA-based therapeutics
and presented it as a biomarker for breast cancer diagnosis, prognosis, and treatment response. In
this regard, proteome analyses provided an understanding of the molecular mechanism underlying
the effect of miR-185-5p on breast cancer.
Dengue Fever Related Reactive Thrombocytosis in Young Male: A Case Report and Review Literature
Authors: Singh Jitendra , Dinkar Anju , Kumar Nilesh , Kumar Kailash , Vikrant  
Published: September 2024

Introduction:
Dengue fever is prevalent in tropical nations, especially India. Leucopenia
and thrombocytopenia are distinctive features of acute dengue fever that revert to normal levels
after the patient's recovery. Dengue fever is associated with numerous unusual clinical manifestations
of different body systems. Additionally, the emergence of severe thrombocytosis following
thrombocytopenia is extremely rare. Based on our extensive knowledge, only three cases similar
to this have been documented in the literature.

Case Report:
Here, we present a case of a 36-year-old healthy man who had acute dengue and subsequently
developed severe reactive thrombocytosis. The patient was treated conservatively and
discharged. Subsequently, he developed thrombocytosis. Aspirin was given for a short period to
alleviate any potential repercussions.

Conclusion:
Thrombocytosis, a rare consequence of dengue infection, is usually asymptomatic.
Nevertheless, ongoing monitoring of dengue patients is required to avoid complications.
Ciências no currículo do Curso Normal Rural da Vila de Santa Izabel, Estado do Pará (1931-1936)
Authors: Nunes Joás Murilo , Barros e Barros Jônatas , De Alencar Alves José Jerônimo 
Published: September 2024

Resumo
O presente artigo teve como objetivo analisar as condições que possibilitaram a inserção das ciências nos programas de ensino do Curso Normal Rural, criado em 1931, situado na vila de Santa Izabel do Estado do Pará. Para atingi-lo, a pesquisa foi norteada pelas seguintes questões: como essas ciências e o curso que as abrigou encontraram condições de se viabilizar na cultura local? Que ciências foram essas, como foram representadas e como se situaram em relação as demais disciplinas no interior dos programas de ensino adotado? O conceito de cultura e de representação cunhados por Hall (2016) foram fundamentais para a análise das fontes historiográficas, tais como, decretos e regulamentos governamentais, programas de ensino e demais discursos que passaram a se inserir na cultura local. Entre essas representações havia as que requeriam a implantação de um ensino rural orientado para a prática de uma agricultura que seria eficiente, intensiva e, ao mesmo tempo, com profundo apreço pela terra e pela natureza, com fins de lidar com o problema do êxodo rural enfrentado na época. As disciplinas científicas que foram adotadas no programa do Curso Normal Rural de Santa Izabel, foram as denominadas: Ciências Naturais, em 1931 e Ciências Físicas e Naturais, em 1934.
Palavras-chave: Curso Normal Rural, Ciências, Cultura, Pará.
Abstract
The aim of this article was to analyze the conditions that made it possible to include the sciences in the teaching programs of the Rural Normal Course, created in 1931, located in the town of Santa Izabel in the state of Pará. To achieve this, the research was guided by the following questions: how did these sciences and the course that housed them find the conditions to become viable in the local culture? What were these sciences, how were they represented and how were they situated in relation to the other subjects within the teaching programs adopted? The concept of culture and representation coined by Hall (2016) was fundamental to the analysis of historiographical sources, such as government decrees and regulations, teaching programs and other discourses that became part of local culture. Among these representations were those that called for the implementation of a rural education oriented towards the practice of agriculture that would be efficient, intensive and, at the same time, with a deep appreciation for the land and nature, in order to deal with the problem of rural exodus faced at the time. The scientific subjects that were adopted in the program of the Santa Izabel Rural Normal Course were: Natural Sciences, in 1931 and Physical and Natural Sciences, in 1934.
Keywords: Rural Normal Course, Science, Culture, Pará.
MODELO TEÓRICO/DIDÁTICO DO GÊNERO TEXTUAL RELATO PESSOAL
Authors: Domingos Marilúcia dos Santos , Vidotti Gabriela Aparecida 
Published: September 2024

Este trabalho tem como objetivo apresentar um modelo teórico/didático do gênero textual relato pessoal. O modelo é uma ferramenta que possibilita a identificação dos elementos que caracterizam um gênero. Para tanto, tomamos como norte o Dispositivo Didático de Gêneros elaborado por Barros (2012) e realizamos pesquisa bibliográfica e exploratória. Os resultados colocaram em evidências as especificidades do gênero, em destaque: a função social de documentar e memorizar ações humanas; pertencimento a diferentes esferas socias de linguagem; o papel social de seus autores; destinatários pretendidos; estrutura flexível; os tempos verbais predominantes, assim como os aspectos que constituem a coesão textual, o tipo de linguagem e o tipo de discurso empregado.
Formulasi Sabun Gel Ekstrak Biji Manjakani Menggunakan Tragakan dan Carbopol 934 sebagai Gelling Agent
Authors: Amalia Anisa , Nursal Fith Khaira , Nining Nining , Bariroh Tahyatul , Laszairina Andra Yunita , Dinaianti Adelia 
Published: September 2024

Biji manjakani (Quercus infectoria) dapat digunakan sebagai bahan aktif pada sediaan sabun pembersih kewanitaan karena mengandung senyawa tannin yang dapat menghambat pertumbuhan jamur Candida albicans. Tragakan dan carbopol 934 merupakan gelling agent yang dapat digunakan pada pembuatan sabun gel. Tujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan jenis dan konsentrasi gelling agent terhadap stabilitas fisik sabun gel pembersih kewanitaan ekstrak etanol 96% biji manjakani. Formula 1 - 4 menggunakan tragakan pada konsentrasi 0,5% (F1), 1% (F2), 1,5% (F3), dan 2% (F4). Formula 5 - 8 menggunakan carbopol 934 pada konsentrasi 0,75% (F5), 1% (F6), 1,25% (F7), dan 1,5% (F8). Uji Stabilitas fisik dilakukan selama 6 minggu pada suhu 300C dan dilakukan pengamatan organoleptis, homogenitas, nilai pH dan viskositas yang dilanjutkan denganuji stabilitas tinggi busa dan uji cycling test. Penggunaan kedua jenis gelling agent menghasilkan sabun gel yang homogen dan stabil secara visual pada penyimpanan pada suhu kamar. Penggunaan tragakan menghasilkan sabun gel dengan viskositas yang lebih tinggi dibandingkan carbopol 934. Nilai pH sabun gel adalah 3,86 – 4,54 dan memenuhi kriteria sabun kewanitaan. Busa yang dihasilkan oleh tiap formula stabil selama masa pengamatan. F1 dan F5 mengalami pemisahan fase pada pengujian cycling test. Penggunaan tragakan pada konsentrasi 1,5% (F3) dan carbopol pada konsentrasi 1,5% (F8) mampu mempertahankan (p > 0,05) nilai pH dan viskositas sediaan selama penyimpanan. F3 dan F8 memiliki respon daya hambat pertumbuhan C. albicans yang lemah.
История становления и развития кафедры инфекционных болезней КБГУ
Authors: Ширитова Л.А. 
Published: September 2024

Статья посвящена созданию и становлению кафедры инфекционных болезней Кабардино-Балкарского государсвтенного университета им. Х.М. Бербекова.
Медицинский факультет
Authors: Захохов Р.М. , Шомахов А.О. , Ахриев Х.Р. 
Published: September 2024

Статья посвящена истории становления и развития медицинского образования в Кабардино-Балкарии, открытию медицинского факультета в Кабардино-Балкарском государственном университете, а также современному состоянию подготовки медицинских кадров в республике.
Effect of Ozone Gas on Selected Microbiological, Chemical, Electrical and Organoleptic Properties of Craft Wheat Beers Enriched Japanese Quince Fruit (Chaenomeles L.)
Authors: Gorzelany Józef , Zardzewiały Miłosz , Findura Pavol , Kapusta Ireneusz , Pentoś Katarzyna , Belcar Justyna 
Published: September 2024

Abstract
Japanese quince fruits (Chaenomeles L.) are a valuable source of bioactive compounds, including polyphenolic compounds, vitamins, macro- and microelements. By using Japanese quince fruit, you can fortify wheat beers and influence the qualitative and sensory values as well as the content of bioactive compounds in the finished product. Sensory evaluation showed that wheat beers with the addition of nonozonated Japanese quince fruits were characterized by a balanced taste and aroma (overall impression), but all wheat beers obtained were characterized by high sensory values, and the ozonation process had a positive effect on the microbiological stability of both fruits and beers with the addition of these fruit. Physico-chemical analysis of wheat beers showed that beers enriched with Japanese quince fruit were characterized by high total acidity, respectively: average values of 4.95 (fruit-free) and 6.66 (0.1 M NaOH/100 mL), higher energy value, and, especially beers enriched with ozonated Japanese quince fruit and high total polyphenol content. It was shown that wheat beers enriched with quince fruit contained mainly chlorogenic acid at an average level of 4.35 and 5.42 mg/L (for ozonated and nonozonated fruit, respectively). Electrical properties (impedance and capacity) allowed the differentiation of the analyzed wheat beers. Based on the conducted research, it appears that wheat beers enriched with Japanese quince fruit may constitute a new trend in the brewing industry.
Physicochemical Characteristics of Biscuits Produced from Gluten-Free Amaranth Seed and Tiger Nut Composite Flour
Authors: Ayeni Funmilola Ayobami , Malomo Adekunbi Adetola , Ikujenlola Abiodun Victor 
Published: September 2024

Abstract
This study was carried out to advance the use of indigenous composite flour obtained from amaranth seed and tiger nut in the production of biscuits to increase access to a healthy and sustainable diet. Biscuit was produced from amaranth seed flour substituted with tiger nut flour at 0%, 10%, 20%, 30%, 40% and 50%. The functional and pasting properties of the flour samples were determined, the proximate composition was evaluated and selected minerals contents of flour and biscuit samples were also assessed using standard methods. The results showed that the protein, fat, ash and fibre content of amaranth seed flour increased from 13.86%, 4.12%, 1.77% and 1.26% respectively to a range of 13.86 – 16.16%, 4.47 – 5.18%, 2.18 – 2.65% and 2.35 – 3.96% respectively with the addition of tiger nut flour. An increase in protein, fat, ash and fibre was also recorded in the biscuit samples with the increased concentration of tiger nut flour. Flour and biscuits produced from 100% wheat had lower protein, fat and fibre content than 100% amaranth seed flour and the blends. The bulk density and water absorption capacity of 100% amaranth seed flour and the blends were significantly higher than 100% wheat flour. Biscuits produced from amaranth seed flour substituted with tiger nut flour at 50% had the highest content of sodium (202.59 mg/100g) and potassium (299.57 mg/100g). In comparison, 100% wheat biscuit and 100% amaranth biscuit had the highest calcium (186.67 mg/1000g) and iron content (4.13 mg/100g) respectively. The study concluded that amaranth seed and tiger nut flour blends could be utilized to produce high-nutritional quality gluten-free biscuits that will increase access to an affordable healthy and sustainable diet.
The Digital Transformation of European Union Countries before and during COVID-19
Authors: Huňady Ján , Pisár Peter , Khawaja Sarwar , Qureshi Fayyaz Hussain 
Published: September 2024

Abstract

Background
The transformation of business and the economy is a key business problem, and its importance was further heightened during the COVID-19 pandemic, with the increased pressure on enterprises. Hence, the digital readiness of their business will likely determine its future competitiveness in the field. Objectives: The paper addresses the issue of digital readiness for business, with the aim of examining similarities and differences in the digital readiness of European Union countries. The main focus is on identifying the changes during the first two years of the pandemic. Methods/Approach: We conducted a factor analysis and hierarchical cluster analysis based on selected indicators. The results revealed some significant differences. Results: While the countries that lead in digital readiness remained the same, there were relatively large differences in the classification of other EU countries. Some countries, such as Latvia or Slovenia, significantly improved in many aspects of digital readiness during the pandemic. On the other hand, Bulgaria and Romania are still included in the same cluster, representing an overall low digital readiness. Conclusion: Enterprises in more developed countries also have a higher level of digital readiness. Although COVID-19 had a positive impact on digital readiness in some less developed countries, the impact is only partial, indicating a need for stronger actions to bridge the digital divide at the EU level. These findings provide a hopeful outlook, suggesting that with the right actions, the digital divide in the EU can be effectively bridged.
Impact of green finance and environmental tax on sustainable development: Evidence from Vietnam
Authors: Le Tran Ha Trang , Nguyen Thi Dieu Chi , Cao Thi Y Nhi , Dang Thi Thuy Hong 
Published: September 2024

Abstract
The worldwide need for environmental preservation and robust economic development has garnered significant interest from scholars and politicians. This study examines the influence of green finance and environmental protection tax on Vietnam’s financial sustainability and the transition towards a green economyas an emerging economy. The study obtained data from the Central Bank of Vietnam and World Bank Indicators covering 2012 to 2022. The authors used the Ordinary Least Squares approach to examine the associations between the variables. The analysis and examination of the data used Stata 19 software. The findings show a favourable correlation between green finance and environmental tax on sustainable development goalswith all control variables. These results have prompted authorities to focus on green finance and environmental issues in the progress towards the country’s green economy and sustainable development.
Collapse of underground galleries in gold-panning mines: Perception of stakeholders in the Doko Sub-Prefecture, Siguiri Prefecture, Republic of Guinea
Authors: Diallo Moussa , Konaté Ahmed Amara , Sidibé Diaka 
Published: September 2024

Abstract
Artisanal gold mining is recognized as a long-standing activity in the Siguiri prefecture in general and the Doko sub-prefecture in particular. Despite its significant contribution to community development, it has disastrous consequences for both the environment and the lives of gold miners. The collapse of underground galleries as a result of gold panning is a phenomenon that plunges families into mourning in gold panning areas in Africa. Unlike other land movements, despite their dangerous nature, there has been almost no scientific interest in limiting the consequences of collapsing underground galleries in goldmines. Working to mitigate their effects is more than a necessity for those involved in gold panning.This study aims to understand the perceptions of those involved in gold panning regarding the conditions of occurrence and the main causes of underground gallery collapses, to formulate recommendations to the authorities and gold miners to minimize the risk of these accidents occurring and their impact. The methodology used was based on semi-structured and structured interviews. The results of this survey show that the human factor is certainly the main cause of accidents (collapse of underground galleries), but the design of structures without taking into account the study of the geomechanical properties of the ground, the weakness of the safety measures put in place, and the lack of involvement of the country’s authorities are not negligible factors. This study identified the causes and consequences of these accidents, as well as the responsibilities of those involved.
Mimetite and polymineralic mimetite-pyromorphite-vanadinite single crystals from the Sowie Mts, Poland
Authors: Szełęg Eligiusz , Janeczek Janusz , Juroszek Rafał , Danila Marta 
Published: September 2024

Abstract
Millimeter-sized crystals of mimetite and pyromorphite, and polymineralic mimetite-pyromorphite-vanadinite crystals occur in quartz-baryte vein within paragneisses of the Sowie Mts, SW Poland. Three morphologically different mimetite crystals and a polymineralic crystal were examined by electron probe micro-analysis (EPMA), back-scattered electrons (BSE) imaging, Raman microspectroscopy, and X-ray composition mapping. Mimetite occurs as well-developed crystals, crystals built up of sub-parallel individuals due to autoepitaxial growth, and crystals extensively etched. All of the mimetite crystals are zoned with respect to pyromorphite molecule content with sharp increase up to 23 mol% in the outermost zones. The apparent vanadinite crystal actually consists of oscillatory-zoned pyromorphite + minor vanadinite core, intermediate zones composed of pyromorphite, two mimetite zones intercalated by a band of oscillatory pyromorphite and minor vanadinite, and vanadinite mantle. EPMA data show a limited miscibility between all three minerals in the polymineralic crystal. Most analyzes cluster around 10 mol% of ternary solid solution with the maximum value of ca. 30 mol%. X-ray elemental maps reveal sharp boundaries between compositionally contrasting zones in the crystal core. In mimetite zones, the substitution of As by P does not exceed 0.43 atoms per formula unit (apfu). In the vanadinite mantle, As + P does not exceed 0.30 apfu. The distribution of Pb is uniform throughout the crystal with the highest Ca/Pb ratio of 0.03. The observed sequence of crystallization in the polymineralic crystal can be explained by the relative changes in ions concentrations at the crystal/solution interface, i.e. within the diffusion boundary layer, in accord with the models of the autocatalytic crystal growth. The authors hypothesize that kinetically driven fast growth of the polymineralic crystals resulted in precipitation of discrete mineral phases with very limited anionic substitutions.
Phytochemical composition and antifungal effectiveness of Phoenix dactylifera L. rachis extracts
Authors: Abdelkhalek Ahmed , Abdelwahab Eman A. , Elalem Saad F. , Al-Askar Abdulaziz A. , Kowalczewski Przemysław Ł. , Behiry Said 
Published: September 2024

Abstract
The present study appraised the inhibitory role of ethanol (PDEE) and ethyl acetate (PDEAE) extracts of Phoenix dactylifera L. against three molecularly identified fungi: Fusarium oxysporum, Botrytis cinerea, and Rhizoctonia solani. HPLC analysis revealed that gallic acid was the major phenolic compound in both extracts: (PDEE: 1721.90 μg/g) and (PDEAE: 101.53 μg/g). The major flavonoids in PDEE are rutin, kaempferol, and quercetin, whereas PDEAE contains kaempferol, naringenin, and quercetin. The GC-MS showed 11-octadecenoic acid methyl ester (26.25%) is the highest compound in PDEE, while diisooctyl phthalate (18.82%) is the most important compound in PDEAE. At 50 μg/mL, the inhibition percentage of PDEAE initiated the highest growth inhibition of F. oxysporum (49.63%) and R. solani (71.43%). Meanwhile, PDEE at 200 μg/mL initiated an inhibition value of 77.78% for B. cinerea. As a result, PDEAE is considered more effective than PDEE in controlling the growth of selected isolates.
Intelligent Models for Prediction of Compressive Strength of Geopolymer Pervious Concrete Hybridized with Agro-Industrial and Construction-Demolition Wastes
Authors: Marathe Shriram , Rodrigues Anisha P 
Published: September 2024

Abstract
In modern civil engineering, precisely predicting the mechanical properties of waste-modified geopolymer concrete is a vital challenge. Machine learning (ML) offers a powerful tool for such predictive analysis. This article presents an experimental and python-based intelligent ML modeling study on a type of geopolymer (GP) pervious concretes developed using agro-industrial waste products. The slag-based composite mixes were developed with the varying dosages of agro-waste, i.e., sugarcane bagasse ash (0 to 20% by weight of slag) and construction and demolition waste in the form of recycled coarse aggregates (0 to 100% by weight of natural aggregates). The aqueous solution of liquid Na2SiO3 and NaOH pellets were used as an alkali activator solution. A total of 13 different mix proportion designs were developed, and for every individual sample mix, the results were obtained from laboratory tests. The ML analysis was carried out to compute the compressive strength by applying following models: Multiple Linear Regression, tuned Gradient Boost, AdaBoost, and XGBoost Regressions. Further, an ensemble technique that combines the predictions from multiple ML algorithms together to make more accurate predictions than any individual model was also developed for a more accurate and robust prediction through the “Voting Regressor” technique. From the analysis of the obtained results, the ML models associated with Ada Boost tuned performed better. As the ensemble voting regressor models were given higher weightage, these regressors gave the best performance metrics, with lower error rate compared to the independent models.