bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sowing a winter catch crop can reduce nitrate leaching losses from winter‐applied urine under simulated forage grazing: a lysimeter study
Authors: Carey P. L. , Cameron K. C. , Di H. J. , Edwards G. R. , Chapman D. F. 
Published: July 2016

AbstractGrazing of winter forage crops is a common management option used in the dairy industry of New Zealand, particularly in the South Island, where they are used to feed nonlactating, pregnant dairy cows prior to calving. However, there is concern that the large crop yields per hectare grazed, combined with a high stocking density of cows, lead to large amounts of urinary nitrogen (N) deposited on bare, wet soil that, in turn, could lead to large nitrate leaching losses. We report the results of a simulated winter forage grazing event using field lysimeters planted with a kale (Brassica oleracea L.) crop. The effect of sowing a ‘catch crop’ of oat (Avena sativa L.) following the simulated winter forage grazing on nitrate leaching losses from urine applied at different times throughout the winter was measured. A catch crop sown between 1 and 63 days after the urine deposition in early winter reduced N leaching losses from urine patches by ~34% on average (range: 19–49%) over the winter–spring period compared with no catch crop. Generally, the sooner the catch crop was sown following the crop harvest, the greater the uptake of N by the catch crop and the greater the reduction in nitrate leaching losses. The results indicate that sowing of a catch crop following winter crop grazing could be an effective management strategy to reduce nitrate leaching as well as increase the N‐use efficiency of dairy winter forage grazing systems.
Weed suppressiveness of paving joint filling materials under diverging water regimes
Authors: De Cauwer B , Faes J , Biesemans N , Claerhout S , Reheul D 
Published: July 2016

SummarySince 2015, chemical weed control on public pavements in Flanders has been banned. This necessitates alternative weed control strategies. In this study, growth chamber experiments evaluated the weed suppressive ability of different joint filling materials under various water regimes. The tested materials comprised five unbound standard fillers (white quartz sand, sea sand, limestone 0/2 mm, limestone 2/6.3 mm and porphyry 2/6.3 mm) and two innovative materials (Dansand® and Eco Fugensand®). Their weed suppressiveness was tested in pure and organically polluted states. Germination and biomass accumulation of two weed species that are abundantly found on public pavements (Lolium perenne and Taraxacum officinale) were investigated. Germination and biomass accumulation were lowest in both innovative materials, irrespective of organic contamination level, plant species and water regime. Weed growth in the standard materials was affected by plant species and water regime. Monthly biomass accumulation increased with increasing monthly water supply and number of irrigation days. Furthermore, the materials best capable of reducing weed growth, under all water regimes, even when organically polluted, were the innovative materials and sea sand. The results of this study show that the implemented water regime can influence weed suppressiveness (absolute as well as relative) of a joint filler. Hence, to fully assess weed suppressive ability, commercially launched joint fillers should be tested under diverging water regimes.
Potential of Using Design-Build in Public Procurement
Authors: Blaťák Ing. Jakub 
Published: July 2016

Risk is an omnipresent effect in every area of human activity. Ignoring it can cause serious problems. This article is about eliminating risk in public contracts using the design-build method as a possible solution to reduce risk. First, we need to compare the standard method of design-bid-build with its alternative, design-build. Two methods are used in this article to analyse risk – the main method is RIPRAN. The evaluated results provided by the RIPRAN method are processed by research data using a scoring method with a risk map. The analysis is going to detect the difference between the DBB and DB methods, primarily in appearance and its impact on the realization of the whole construction. The comparison identifies numerous contractual topics and risks included in both and gives deeper insight into risk management, both for the contracting party and also for public procurement. Applying risk analysis strategies and tools to the process will help decision-makers evaluate and select the most suitable delivery method consistently and defensibly. This paper gives generic risk factors related to both project types.
Verification and Validation in Fluids Engineering: Some Current
Issues
Authors: Roache Patrick J. 
Published: July 2016

Abstract
The following current issues in verification and validation (V&V) are discussed: probability bounds analysis (PBA) versus the traditional probability distribution function (PDF) approach as used in the ANSI Standard document ASME V&V 20-2009; strict frequentist versus Bayesian approaches; model form uncertainty (and confusion) in prediction versus validation; Popper's philosophical falsificationism versus common sense; climate model trend validations versus climate change deniers; and V&V state of the art versus practice. Also, the many fundamental contributions to modern V&V practice in the history of the ASME Fluids Engineering Division and the Journal of Fluids Engineering (JFE) are described.
Draft Genome Sequence of
Halobacillus
sp. Strain KGW1, a Moderately Halophilic and Alkaline Protease-Producing Bacterium Isolated from the Rhizospheric Region of
Phragmites karka
from Chilika Lake, Odisha, India
Authors: Panda Ananta Narayan , Mishra Samir R. , Ray Lopamudra , Sahu Neha , Acharya Ankita , Jadhao Sudhir , Suar Mrutyunjay , Adhya Tapan Kumar , Rastogi Gurdeep , Pattnaik Ajit Kumar , Raina Vishakha 
Published: July 2016

ABSTRACT

Halobacillus
sp. strain KGW1 is a moderately halophilic, rod shaped, Gram-positive, yellow pigmented, alkaline protease-producing bacterium isolated from a water sample from Chilika Lake, Odisha, India. Sequencing of bacterial DNA assembled a 3.68-Mb draft genome. The genome annotation analysis showed various gene clusters for tolerance to stress, such as elevated pH, salt concentration, and toxic metals.
Draft Genome Sequence of a New Zealand Rickettsia-Like Organism Isolated from Farmed Chinook Salmon
Authors: Gias Edna , Draper Jenny , Brosnahan Cara L. , Orr Della , McFadden Andrew , Jones Brian 
Published: July 2016

ABSTRACT
We report here the draft genome sequence of a rickettsia-like organism, isolated from a New Zealand Chinook salmon farm experiencing high mortality. The genome is approximately 3 Mb in size, has a G+C content of approximately 39.2%, and is predicted to contain 2,870 coding sequences.
Genome Sequences of Gordonia terrae Phages Attis and SoilAssassin
Authors: Pope Welkin H. , Biery Daniel N. , Huff Zachary T. , Huynh Amy B. , McFadden William M. , Mouat Julia S. , Schneiderman Scott E. , Song Hannah , Szpak Leah E. , Umbaugh Melanie S. , German Brian A. , McDonnell Jill E. , Mezghani Nadia , Schafer Claire E. , Thompson Paige K. , Ulbrich Megan C. , Yu Victor J. , Furbee Emily C. , Grubb Sarah R. , Warner Marcie H. , Montgomery Matthew T. , Garlena Rebecca A. , Russell Daniel A. , Jacobs-Sera Deborah , Hatfull Graham F. 
Published: July 2016

ABSTRACT

Attis and SoilAssassin are two closely related bacteriophages isolated on
Gordonia terrae
3612 from separate soil samples in Pittsburgh, PA. The Attis and SoilAssassin genomes are 47,881 bp and 47,880 bp, respectively, and have 74 predicted protein-coding genes, including toxin-antitoxin systems, but no tRNAs.
Complete Genome Sequence of Aurantimicrobium minutum Type Strain KNC
T
, a Planktonic Ultramicrobacterium Isolated from River Water
Authors: Nakai Ryosuke , Fujisawa Takatomo , Nakamura Yasukazu , Nishide Hiroyo , Uchiyama Ikuo , Baba Tomoya , Toyoda Atsushi , Fujiyama Asao , Naganuma Takeshi , Niki Hironori 
Published: July 2016

ABSTRACT

Aurantimicrobium minutum
type strain KNC
T
is a planktonic ultramicrobacterium isolated from river water in western Japan. Strain KNC
T
has an extremely small, streamlined genome of 1,622,386 bp comprising 1,575 protein-coding sequences. The genome annotation suggests that strain KNC
T
has an actinorhodopsin-based photometabolism.
Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater
Authors: Teramoto Maki , Zhai Zhenyu , Komatsu Ayumi , Shibayama Keigo , Suzuki Masato 
Published: July 2016

ABSTRACT

Some bacterial species of the genus
Tenacibaculum
, including
Tenacibaculum ovolyticum
, have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for
Tenacibaculum dicentrarchi
, which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of
T. ovolyticum
DSM 18103
T
, was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus
Tenacibaculum
.
Low prevalence of fetal-type posterior cerebral artery in patients with basilar tip aneurysms
Authors: Diogo Mariana C , Fragata Isabel , Dias Sara P , Nunes Joana , Pamplona Jaime , Reis João 
Published: July 2016

BackgroundBasilar tip aneurysms (BTA) are multifactorial in origin, with luminal forces playing a major role in their formation. Considering the reduced hemodynamic stress on the basilar apex in the fetal-type posterior cerebral artery (fPCA), we hypothesize that BTA should be less common in patients with this variant.ObjectiveTo investigate, in a retrospective case–control study, the frequency of fPCA in patients with and without BTA.Materials and methodsWe collected clinical and imaging data from consecutive patients with BTA undergoing catheter angiography between July 2010 and July 2015, and from a randomly selected, age- and sex-matched non-BTA control population from our prospective database. Anatomical variants of the distal basilar artery region were assessed in the two groups and compared using parametric and non-parametric tests.ResultsFifty-nine BTA cases and 337 controls were included. fPCA was present in 3% of patients with BTA and 23% in the control group (p<0.001; OR=0.11, 95% CI 0.03 to 0.48). Basilar tip disposition was cranial in 49% of BTA and 63% of non-BTA cases (p=0.04; OR=0.57, 95% CI 0.33 to 0.99); a caudal disposition was found in 24% and 6% of cases, respectively (p<0.001; OR=4.65, 95% CI 2.21 to 9.80).ConclusionsWe found a statistically significant association between the absence of fPCA and BTA. Our findings underline the importance of hemodynamic stress in the formation of intracranial aneurysms, and suggest that fPCA is a protective variant for formation of BTA.
BAFF and APRIL from Activin A–Treated Dendritic Cells Upregulate the Antitumor Efficacy of Dendritic Cells In Vivo
Authors: Shurin Michael R. , Ma Yang , Keskinov Anton A. , Zhao Ruijing , Lokshin Anna , Agassandian Marianna , Shurin Galina V. 
Published: July 2016

Abstract
The members of the TGFβ superfamily play a key role in regulating developmental and homeostasis programs by controlling differentiation, proliferation, polarization, and survival of different cell types. Although the role of TGFβ1 in inflammation and immunity is well evident, the contribution of other TGFβ family cytokines in the modulation of the antitumor immune response remains less documented. Here we show that activin A triggers SMAD2 and ERK1/2 pathways in dendritic cells (DC) expressing type I and II activin receptors, and upregulates production of the TNFα family cytokines BAFF (TALL-1, TNFSF13B) and APRIL (TALL-2, TNFSF13A), which is blocked by SMAD2 and ERK1/2 inhibitors, respectively. BAFF and APRIL derived from activin A–treated DCs upregulate proliferation and survival of T cells expressing the corresponding receptors, BAFF-R and TACI. In vivo, activin A–stimulated DCs demonstrate a significantly increased ability to induce tumor-specific CTLs and inhibit the growth of melanoma and lung carcinoma, which relies on DC-derived BAFF and APRIL, as knockdown of the BAFF and APRIL gene expression in activin A–treated DCs blocks augmentation of their antitumor potential. Although systemic administration of activin A, BAFF, or APRIL for the therapeutic purposes is not likely due to the pluripotent effects on malignant and nonmalignant cells, our data open a novel opportunity for improving the efficacy of DC vaccines. In fact, a significant augmentation of the antitumor activity of DC pretreated with activin A and the proven role of DC-derived BAFF and APRIL in the induction of antitumor immunity in vivo support this direction. Cancer Res; 76(17); 4959–69. ©2016 AACR.
Metabolic Stress-Induced Phosphorylation of KAP1 Ser473 Blocks Mitochondrial Fusion in Breast Cancer Cells
Authors: Cheng Chun-Ting , Kuo Ching-Ying , Ouyang Ching , Li Chien-Feng , Chung Yiyin , Chan David C. , Kung Hsing-Jien , Ann David K. 
Published: July 2016

Abstract
Mitochondrial dynamics during nutrient starvation of cancer cells likely exert profound effects on their capability for metastatic progression. Here, we report that KAP1 (TRIM28), a transcriptional coadaptor protein implicated in metastatic progression in breast cancer, is a pivotal regulator of mitochondrial fusion in glucose-starved cancer cells. Diverse metabolic stresses induced Ser473 phosphorylation of KAP1 (pS473-KAP1) in a ROS- and p38-dependent manner. Results from live-cell imaging and molecular studies revealed that during the first 6 to 8 hours of glucose starvation, mitochondria initially underwent extensive fusion, but then subsequently fragmented in a pS473-KAP1-dependent manner. Mechanistic investigations using phosphorylation-defective mutants revealed that KAP1 Ser473 phosphorylation limited mitochondrial hyperfusion in glucose-starved breast cancer cells, as driven by downregulation of the mitofusin protein MFN2, leading to reduced oxidative phosphorylation and ROS production. In clinical specimens of breast cancer, reduced expression of MFN2 corresponded to poor prognosis in patients. In a mouse xenograft model of human breast cancer, there was an association in the core region of tumors between MFN2 downregulation and the presence of highly fragmented mitochondria. Collectively, our results suggest that KAP1 Ser473 phosphorylation acts through MFN2 reduction to restrict mitochondrial hyperfusion, thereby contributing to cancer cell survival under conditions of sustained metabolic stress. Cancer Res; 76(17); 5006–18. ©2016 AACR.
Systemic DC Activation Modulates the Tumor Microenvironment and Shapes the Long-Lived Tumor-Specific Memory Mediated by CD8+ T Cells
Authors: Shimizu Kanako , Yamasaki Satoru , Shinga Jun , Sato Yusuke , Watanabe Takashi , Ohara Osamu , Kuzushima Kiyotaka , Yagita Hideo , Komuro Yoshiko , Asakura Miki , Fujii Shin-ichiro 
Published: July 2016

Abstract
Strategies to reprogram the tumor microenvironment are being explored to improve cancer immunotherapy. In one approach, we have targeted dendritic cells (DC) to improve their function with adjuvant vector cells (aAVC) that are engineered from NKT ligand-loaded CD1d+ allogeneic cells transfected with tumor antigen mRNAs. Here, we report the finding that this approach also programs local immune responses by establishing tertiary lymphoid structures (TLS), which include expanded antigen-specific CD8+ T-cell clones, mobilized DCs, and normalized tumor vasculature. aAVC therapy also expanded specific Vβ-expressing antitumor T-cell clones, leading to the formation of long-term memory T cells. When combined with PD-1 blockade, aAVC infusion triggered regression of poorly immunogenic tumor cells that did not respond to PD-1 blockade alone, as well as expansion of antigen-specific CD8+ T-cell clones in the tumor. The findings of this study help to inform a next-generation platform for the generation of efficacious cancer vaccines. Cancer Res; 76(13); 3756–66. ©2016 AACR.
Tyrosine Phosphatase PTPRJ/DEP-1 Is an Essential Promoter of Vascular Permeability, Angiogenesis, and Tumor Progression
Authors: Fournier Patrick , Dussault Sylvie , Fusco Alfredo , Rivard Alain , Royal Isabelle 
Published: July 2016

Abstract
The protein tyrosine phosphatase PTPRJ/DEP-1 has been implicated in negative growth regulation in endothelial cells, where its expression varies at transitions between proliferation and contact inhibition. However, in the same cells, DEP-1 has also been implicated in VEGF-dependent Src activation, permeability, and capillary formation, suggesting a positive role in regulating these functions. To resolve this dichotomy in vivo, we investigated postnatal angiogenesis and vascular permeability in a DEP-1–deficient mouse. In this study, we report that DEP-1 is required for Src activation and phosphorylation of its endothelial cell–specific substrate, VE-cadherin, after systemic injection of VEGF. Accordingly, VEGF-induced vascular leakage was abrogated in the DEP-1–deficient mice. Furthermore, capillary formation was impaired in murine aortic tissue rings or Matrigel plugs infused with VEGF. In the absence of DEP-1, angiogenesis triggered by ischemia or during tumor formation was defective, which in the latter case was associated with reduced tumor cell proliferation and increased apoptosis. Macrophage infiltration was also impaired, reflecting reduced vascular permeability in the tumors or a possible cell autonomous effect of DEP-1. Consequently, the formation of spontaneous and experimental lung metastases was strongly decreased in DEP-1–deficient mice. In clinical specimens of cancer, less vascularized tumors exhibited lower microvascular expression of DEP-1. Altogether, our results established DEP-1 as an essential driver of VEGF-dependent permeability, angiogenesis, and metastasis, suggesting a novel therapeutic route to cancer treatment. Cancer Res; 76(17); 5080–91. ©2016 AACR.
CDK4/6 Inhibitors: Promising Opportunities beyond Breast Cancer
Authors: Lim Joline S.J. , Turner Nicholas C. , Yap Timothy A. 
Published: July 2016

Abstract
Summary: Patnaik and colleagues report on the safety, pharmacokinetics, pharmacodynamics, and preliminary efficacy of abemaciclib for the treatment of advanced solid cancers, demonstrating antitumor activity in advanced breast cancers as well as glioblastoma, melanoma, non–small cell lung cancer, colorectal cancer, and ovarian cancer. The development of abemaciclib and other CDK4/6 inhibitors should now be fully optimized through the use of novel predictive biomarkers of response and rational combinations. Cancer Discov; 6(7); 697–9. ©2016 AACR.
See related article by Patnaik et al., p. 740.
Fuzzy Logic Controlled Harmonic Suppressor in Cascaded Multilevel Inverter
Authors: Kameswari Y. Lalitha , Sekhar O. Chandra 
Published: July 2016

<p>This paper presents an investigation of seven level cascaded H-bridge (CHB) inverter in power system for compensation of harmonics.For power quality control a Fuzzy Logic Control (FLC) giving comparatively better harmonic reduction than the conventional controllers. Harmonic distortion is the most important power quality problem stirring in multilevel inverter, the harmonics can be eliminated by an optimal selection of switching angles. A hybrid evaluation technique evaluates the obtained optimal switching angles that are attained from the fuzzy inference system as well as neural network. The proposed method will be implemented in MATLAB working platform and the harmonic elimination performance will be evaluated.</p>
O DIREITO FUNDAMENTAL DE ACESSO A MEDICAMENTOS E A FUNÇÃO SOCIAL DA PROPRIEDADE IMATERIAL NO BRASIL
Authors: Batista Cláudia Karina Ladeia , Calil Mário Lúcio Garcez 
Published: July 2016

O objetivo do presente trabalho é estudar as relações entre o direito de acesso a medicamentos e o direito de exclusividade na exploração de inventos farmacêuticos, a partir do direito fundamental à saúde em suas várias dimensões. Este objetivo será buscado por intermédio de pesquisa bibliográfica e documental. O presente estudo é justificado tendo em vista que o acesso a medicamentos constitui, atualmente, parcela indispensável do direito fundamental à saúde, devendo ser equilibrado com o direito de patente, considerando-se que toda propriedade, inclusive a imaterial, tem uma função social. Conclui-se que é necessário harmonizar o direito à preservação da propriedade imaterial – e, consequentemente, à exclusividade na exploração econômica do produto desenvolvido e patenteado – com o direito de acesso aos medicamentos, sob pena de se esvaziarem ambas as garantias constitucionais.
O PODER NORMATIVO DAS COMISSÕES INTERGESTORES BIPARTITE E A EFETIVIDADE DE SUAS NORMAS
Authors: Leão Thiago Marques , Dallari Sueli Gandolfi 
Published: July 2016

O objetivo deste artigo é discutir o poder normativo da Comissão Intergestores Bipartite (CIB) e a efetividade de suas normas, no bojo do Direito Sanitário brasileiro. Metodologicamente, adotou-se a análise bibliográfica e legislativa, articulada à teoria discursiva do direito e democracia de Habermas. A CIB foi instituída pela Norma Operacional Básica 01/1993 e responde pelos aspectos operacionais do Sistema Único de Saúde (SUS). É um espaço de negociação e pactuação entre gestores municipais e estaduais, promovendo a integração dos diferentes sistemas municipais de saúde sob coordenação do governo estadual. As normas expedidas pela CIB, na forma de resoluções, têm natureza jurídica de direitos-meio, isto é, estabelecem os procedimentos específicos para realização do direito à saúde, permitindo que todos os potenciais destinatários dessas normas possam conhecer, aderir, criticar ou mesmo judicializá-las. O poder normativo da CIB decorre da previsão legislativa expressa para regular os aspectos operacionais do SUS e da legitimidade democrática, exercida diretamente pelo cidadão ou através dos Conselhos Estaduais de Saúde (CES). Caracterizado como fórum de negociação e operacionalização das políticas de saúde, com potencial para abrir e aprofundar canais de participação e controle social, é um espaço de gênese democrática de direitos, e suas normas devem ser, portanto, respeitadas por integrarem o ordenamento jurídico e cumprirem sua função de instrumentalização normativa do SUS.