bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraflagellar transport protein IFT52 recruits IFT46 to the basal body and flagella
Authors: Lv Bo , Wan Lei , Taschner Michael , Cheng Xi , Lorentzen Esben , Huang Kaiyao 
Published: March 2017

ABSTRACT
Cilia are microtubule-based organelles and perform motile, sensing and signaling functions. The assembly and maintenance of cilia depend on intraflagellar transport (IFT). Besides ciliary localization, most IFT proteins accumulate at basal bodies. However, little is known about the molecular mechanism of basal body targeting of IFT proteins. We first identified the possible basal body-targeting sequence in IFT46 by expressing IFT46 truncation constructs in an ift46-1 mutant. The C-terminal sequence between residues 246–321, termed BBTS3, was sufficient to target YFP to basal bodies in the ift46-1 strain. Interestingly, BBTS3 is also responsible for the ciliary targeting of IFT46. BBTS3::YFP moves bidirectionally in flagella and interacts with other IFT complex B (IFT-B) proteins. Using IFT and motor mutants, we show that the basal body localization of IFT46 depends on IFT52, but not on IFT81, IFT88, IFT122, FLA10 or DHC1b. IFT52 interacts with IFT46 through residues L285 and L286 of IFT46 and recruits it to basal bodies. Ectopic expression of the C-terminal domain of IFT52 in the nucleus resulted in accumulation of IFT46 in nuclei. These data suggest that IFT52 and IFT46 can preassemble as a complex in the cytoplasm, which is then targeted to basal bodies.
Ppn2, a novel Zn2+-dependent polyphosphatase in the acidocalcisome-like yeast vacuole
Authors: Gerasimaitė Rūta , Mayer Andreas 
Published: March 2017

ABSTRACT
Acidocalcisome-like organelles are found in all kingdoms of life. Many of their functions, such as the accumulation and storage of metal ions, nitrogen and phosphate, the activation of blood clotting and inflammation, depend on the controlled synthesis and turnover of polyphosphate (polyP), a polymer of inorganic phosphate linked by phosphoric anhydride bonds. The exploration of the role of acidocalcisomes in metabolism and physiology requires the manipulation of polyP turnover, yet the complete set of proteins responsible for this turnover is unknown. Here, we identify a novel type of polyphosphatase operating in the acidocalcisome-like vacuoles of the yeast Saccharomyces cerevisiae, which we called Ppn2. Ppn2 belongs to the PPP-superfamily of metallophosphatases, is activated by Zn2+ ions and exclusively shows endopolyphosphatase activity. It is sorted to vacuoles via the multivesicular body pathway. Together with Ppn1, Ppn2 is responsible for a substantial fraction of polyphosphatase activity that is necessary to mobilize polyP stores, for example in response to phosphate scarcity. This finding opens the way to manipulating polyP metabolism more profoundly and deciphering its roles in phosphate and energy homeostasis, as well as in signaling.
Pharmacological inhibition of the spliceosome subunit SF3b triggers exon junction complex-independent nonsense-mediated decay
Authors: Carvalho Teresa , Martins Sandra , Rino José , Marinho Sérgio , Carmo-Fonseca Maria 
Published: March 2017

ABSTRACT
Spliceostatin A, meayamycin, and pladienolide B are small molecules that target the SF3b subunit of the spliceosomal U2 small nuclear ribonucleoprotein (snRNP). These compounds are attracting much attention as tools to manipulate splicing and for use as potential anti-cancer drugs. We investigated the effects of these inhibitors on mRNA transport and stability in human cells. Upon splicing inhibition, unspliced pre-mRNAs accumulated in the nucleus, particularly within enlarged nuclear speckles. However, a small fraction of the pre-mRNA molecules were exported to the cytoplasm. We identified the export adaptor ALYREF as being associated with intron-containing transcripts and show its requirement for the nucleo-cytoplasmic transport of unspliced pre-mRNA. In contrast, the exon junction complex (EJC) core protein eIF4AIII failed to form a stable complex with intron-containing transcripts. Despite the absence of EJC, unspliced transcripts in the cytoplasm were degraded by nonsense-mediated decay (NMD), suggesting that unspliced transcripts are degraded by an EJC-independent NMD pathway. Collectively, our results indicate that although blocking the function of SF3b elicits a massive accumulation of unspliced pre-mRNAs in the nucleus, intron-containing transcripts can still bind the ALYREF export factor and be transported to the cytoplasm, where they trigger an alternative NMD pathway.
Reframing the science and policy of nicotine, illegal drugs and alcohol – conclusions of the ALICE RAP Project
Authors: Anderson Peter , Berridge Virginia , Conrod Patricia , Dudley Robert , Hellman Matilda , Lachenmeier Dirk , Lingford-Hughes Anne , Miller David , Rehm Jürgen , Room Robin , Schmidt Laura , Sullivan Roger , Ysa Tamyko , Gual Antoni 
Published: March 2017

In 2013, illegal drug use was responsible for 1.8% of years of life lost in the European Union, alcohol was responsible for 8.2% and tobacco for 18.2%, imposing economic burdens in excess of 2.5% of GDP. No single European country has optimal governance structures for reducing the harm done by nicotine, illegal drugs and alcohol, and existing ones are poorly designed, fragmented, and sometimes cause harm. Reporting the main science and policy conclusions of a transdisciplinary five-year analysis of the place of addictions in Europe, researchers from 67 scientific institutions addressed these problems by reframing an understanding of addictions. A new paradigm needs to account for evolutionary evidence which suggests that humans are biologically predisposed to seek out drugs, and that, today, individuals face availability of high drug doses, consequently increasing the risk of harm. New definitions need to acknowledge that the defining element of addictive drugs is ‘heavy use over time’, a concept that could replace the diagnostic artefact captured by the clinical term ‘substance use disorder’, thus opening the door for new substances to be considered such as sugar. Tools of quantitative risk assessment that recognize drugs as toxins could be further deployed to assess regulatory approaches to reducing harm. Re-designed governance of drugs requires embedding policy within a comprehensive societal well-being frame that encompasses a range of domains of well-being, including quality of life, material living conditions and sustainability over time; such a frame adds arguments to the inappropriateness of policies that criminalize individuals for using drugs and that continue to categorize certain drugs as illegal. A health footprint, modelled on the carbon footprint, and using quantitative measures such as years of life lost due to death or disability, could serve as the accountability tool that apportions responsibility for who and what causes drug-related harm.
Children who stutter find their voice onstage and off: The SAY approach to stuttering
Authors: Mareneck Ellen 
Published: March 2017

Abstract
Children who stutter (CWS) face enormous challenges: from bullying and peer rejection to embarrassment, shame and despair. Often these young people shut down and retreat into silence. The Stuttering Association for the Young (SAY), a nonprofit organization in New York City, is dedicated to improving the lives of CWS. SAY uses the performing arts, speech therapy and a sleep-away camp to create community, nurture the individual, and advocate for CWS through education and performance. This article examines SAY as a therapeutic model for Children and Adolescents Who Stutter. SAY’s three-pronged approach of empowerment, education, and support provides CWS with a much-needed community in which they find self-acceptance, communicative confidence, and the courage to achieve their goals.
BUDAYA POPULER GAME POKEMON GO SEBAGAI SOFT DIPLOMACY JEPANG
Authors: Iqbal Chadijah Isfariani 
Published: March 2017

Pokemon Go is a smartphone game that blends the real and digital worlds, tasking players with exploring their neighborhoods to find creatures and treasure for in-game use. This research describes the ways Japan uses its soft power in international coorporation relations, particulary through Pokemon Go’s game. Based on Joseph S. Nye, Jr, the soft power defined as the ability of country to achieve its goal using cultural attraction rather than coercion and violence. After the World War II, Japan has tried to change its image as war crime through popular culture, such as anime, manga and cosplay. According to Nye, Japan has more potential resources in soft power compared to the other countries. This research is focus on Popular Culture of Pokemon Go’s Game as Japan’s Soft Diplomacy. Pokemon Go is one of Japan’s cultural diplomacy activities and the other countries uses popular culture to strengthen the positive image of Japan in the international world. The development of popular culture as a soft power and soft diplomacy also a diplomacy tool of development that can be used by Japan in conducting foreign policy in relation to international cooperation.
International Tax Avoidance – Introduction
Authors: Avi-Yonah Reuven 
Published: March 2017

Abstract
Tax avoidance and evasion is a hot topic. On the evasion (illegal activity by individuals) front, the various leaks culminating in the Panama Papers have once again revealed the scope of evasion by the global elite. Gabriel Zucman conservatively estimated the annual revenue loss at $200 billion. On the tax avoidance (legal activity by corporations) front, the OECD BEPS project has estimated the scope of avoidance by multinationals at between $100 and $240 billion per year. By comparison, total US corporate tax revenues are about $400 billion per year. The articles in this volume reflect various aspects of these troubling phenomena (from the perspective of citizens who pay their tax bills and bear the burden of budget cuts by governments starved of revenues). Yuri Biondi writes from an accounting perspective about the firm as an Enterprise Entity and the tax avoidance conundrum. Matthias Thiemann and Tim Buettner discuss the political economy of the OECD BEPS project. David Quentin discusses tax avoidance in transnational supply chains of multinationals. Blazej Kuzniacki analyzes tax avoidance in EU law, which has been the focus of a lot of activity recently (e. g., the proposed Anti-Tax Avoidance Directive by the EU Commission). Amir Pichhadze and myself discuss the idea of statutory General Anti-Abuse Rule (GAAR) in the US and Canadian contexts.
Well-posedness and maximum principles for lattice reaction-diffusion equations
Authors: Slavík Antonín , Stehlík Petr , Volek Jonáš 
Published: March 2017

Abstract
Existence, uniqueness and continuous dependence results together with
maximum principles represent key tools in the analysis of lattice reaction-diffusion equations. In this paper, we study these questions in full generality by considering nonautonomous reaction functions, possibly nonsymmetric diffusion and continuous, discrete or mixed time. First, we prove the local existence and global uniqueness of bounded solutions, as well as the continuous dependence of solutions on the underlying time structure and on initial conditions. Next, we obtain the weak maximum principle which enables us to get the global existence of solutions. Finally, we provide the strong maximum principle which exhibits an interesting dependence on the time structure. Our results are illustrated by the autonomous Fisher and Nagumo lattice equations and a nonautonomous logistic population model with a variable carrying capacity.
Interior Estimates for Generalized Forchheimer Flows of Slightly Compressible Fluids
Authors: Hoang Luan T. , Kieu Thinh T. 
Published: March 2017

Abstract
The generalized Forchheimer flows are studied for slightly compressible fluids in porous media with time-dependent Dirichlet boundary data for the pressure. No restrictions are imposed on the degree of the Forchheimer polynomial. We derive, for all time, the interior

L
∞

{L^{\infty}}

-estimates for the pressure, its gradient and time derivative, and the interior

L
2

{L^{2}}

-estimates for its Hessian. The De Giorgi and Ladyzhenskaya–Uraltseva iteration techniques are used taking into account the special structures of the equations for both pressure and its gradient. These are combined with the uniform Gronwall-type bounds in establishing the asymptotic estimates when time tends to infinity.
Effect of Climatic Conditions on Differences in Thermo-Technical Properties of Organic Bedding in Laboratory Testing
Authors: Lendelová Jana , Karandušovská Ingrid , Žitňák Miroslav , Boďo Štefan , Mihina Štefan 
Published: March 2017

Abstract
The aim of this experiment was to analyse the influence of climatic factors on the thermal performance of separated liquid manure. The samples of organic bedding were collected from the lying area of dairy housing and preparatory store. The measurement of properties of recycled manure solids (RMS) was carried out in laboratory conditions. Samples were examined with determining the temperature effect on the thermal conductivity λ of ’dry‘ separated liquid manure (with a dry matter content of 60%) and ’wet‘ liquid manure (with a dry matter content of 26%), in air temperatures ranging from 0 °C to 40 °C. Subsequently, the thermal resistance of three selected types of cows lying structures was calculated for winter and summer boundary values. Based on the results of thermal conductivity and thermal resistance, it was observed that dry separated liquid manure as an alternative bedding has, in low temperature (up to 5 °C), the most suitable thermal performance if it is maintained in a dry condition (with a dry matter content of 60%). With increasing temperature (above 20 °C) and increasing humidity of bedding (below 26%), thermo-technical properties get worse; however, when comparing the thermal resistance of the cow bed structure with sandwich mattresses with a 50 mm layer of organic bedding and the deep cubicle filled with 200 mm of organic bedding, the thermal resistance of floor structure decreases by 7.6% or 18.4%, respectively. With a proper handling of the recycled organic bedding, it is possible to use its economic advantages.
Parametric Study of NTH Order Distributed Activation Energy Model for Isothermal Pyrolysis of Forest Waste Using Gaussian Distribution
Authors: Dhaundiyal Alok , Singh Suraj B. 
Published: March 2017

Abstract
This paper deals with the influence of some factors relevant to isothermal pyrolysis of residual leaves of Cedrus deodara on the asymptotic solution of the non-isothermal nth order distributed activation energy model (DAEM) using Gaussian distribution. Frequency factor, integral upper limit, the reaction order and the variance of Gaussian distribution are the parameters taken under purview of this study. In order to determine the kinetic parameters of the isothermal nth order Gaussian DAEM from thermoanalytical data of loose biomass pyrolysis, the variation of these factors has been considered. The obtained results show that the predicted results for nth order DAEM hold good at upper limit of dE, E∞ = 39 kJ mol-1.
Laying Performance, Survival Rate, Egg Quality and Shell Characteristics in Laying Pullets Offered Honey in Drinking Water during Hot Season
Authors: Abioja Monsuru Oladimeji , Adekunle Maryam Oluwakemi , Abiona John Adesanya , Sodipe Olubunmi Grace , Jegede Adebayo Vincent 
Published: March 2017

Abstract
Egg production, survival, egg quality and shell characteristics in laying pullets offered honey in drinking water during hot season were determined using hens (28-week-old, n = 120) allotted to either 0 (CONTROL), 10 (10H) or 20 ml honey/L water (20H) for 16 weeks divided into 4 phases of 4 weeks each. Data on hen-day production (EP), egg weight (EW), length (EL), breadth (EB), shape index (ESI), yolk weight (YW), yolk percentage (YP), albumen height (AH), albumen weight (AW), albumen percentage (AP), Haugh unit (HU), shell weight (SW), shell percentage (SP), shell thickness (ST) and survival (SR) were subjected to ANOVA.
EP was significantly (P < 0.001) affected by treatment during phases 1-4. 20H resulted in higher EP than CONTROL in phase 1. In phase 2, EP was similar in CONTROL and 20H, but 10H was lower than the two. Higher EPs were recorded in CONTROL than in honey groups (phases 3, 4). EW was significantly (P < 0.01) affected by honey in week 1 only. Birds on 10H laid heavier eggs than CONTROL. 10H hens laid significantly longer (P < 0.001) eggs with higher (P = 0.008) ESI than control hens while 20H birds had significantly lower values of AH (P < 0.01), AP (P = 0.05) and HU (P = 0.05). Honey had no significant (P > 0.05) effect on EB, YW, YP, AW, SW, SP and SR, but improved (P < 0.05) ST in the first 2 phases. To ensure improvement in egg production and egg shell thickness in laying pullets during hot season, honey at 20 ml/L water can be offered for 4 weeks.
Decentralized safety concept for closed-loop controlled intensive care
Authors: Kühn Jan , Brendle Christian , Stollenwerk André , Schweigler Martin , Kowalewski Stefan , Janisch Thorsten , Rossaint Rolf , Leonhardt Steffen , Walter Marian , Kopp Rüdger 
Published: March 2017

Abstract:This paper presents a decentralized safety concept for networked intensive care setups, for which a decentralized network of sensors and actuators is realized by embedded microcontroller nodes. It is evaluated for up to eleven medical devices in a setup for automated acute respiratory distress syndrome (ARDS) therapy. In this contribution we highlight a blood pump supervision as exemplary safety measure, which allows a reliable bubble detection in an extracorporeal blood circulation. The approach is validated with data of animal experiments including 35 bubbles with a size between 0.05 and 0.3 ml. All 18 bubbles with a size down to 0.15 ml are successfully detected. By using hidden Markov models (HMMs) as statistical method the number of necessary sensors can be reduced by two pressure sensors.
Identification and classification of upper limb motions using PCA
Authors: Veer Karan , Vig Renu 
Published: March 2017

Abstract:
This paper describes the utility of principal component analysis (PCA) in classifying upper limb signals. PCA is a powerful tool for analyzing data of high dimension. Here, two different input strategies were explored. The first method uses upper arm dual-position-based myoelectric signal acquisition and the other solely uses PCA for classifying surface electromyogram (SEMG) signals. SEMG data from the biceps and the triceps brachii muscles and four independent muscle activities of the upper arm were measured in seven subjects (total dataset=56). The datasets used for the analysis are rotated by class-specific principal component matrices to decorrelate the measured data prior to feature extraction.
Usage-based linguistics and the magic number four
Authors: Green Clarence 
Published: March 2017

AbstractMiller’s (1956, The magical number seven, plus or minus two: Some limits on our capacity for processing information.Psychological Review63(2). 81–97) working memory (WM) capacity of around seven items, plus or minus two, was never found by usage-based linguists to be a recurrent pattern in language. Thus, it has not figured prominently in cognitive models of grammar. Upon reflection, this is somewhat unusual, since WM has been considered a fundamental cognitive domain for information processing in psychology, so one might have reasonably expected properties such as capacity constraints to be reflected in language use and structures derived from use. This paper proposes that Miller’s (1956) number has not been particularly productive in usage-based linguistics because it turns out to have been an overestimate. A revised WM capacity has now superseded it within cognitive science, a “magic number four plus or minus one” (Cowan 2001, The magical number 4 in short-term memory: A reconsideration of mental storage capacity.Behavioral and Brain Sciences24(1). 87–185). This paper suggests, drawing on evidence from spoken language corpora and multiple languages, that a range of linguistic structures and patterns align with this revised capacity estimate, unlike Miller’s (1956), ranging from phrasal verbs, idioms, n-grams, the lengths of intonation units and some abstract grammatical properties of phrasal categories and clause structure.
Demographics, Migration and Brain Drain in The Danube Region
Authors: Savić Mirko , Dakić Stojanka 
Published: March 2017

Abstract
In the last two decades we have been witnessing the decrease of population in many countries of the Danube Region. All demographic indicators are unfavourable. Current demographic situation and labour market in the countries of the Danube Region is presented. The purpose of this paper is to evaluate the effects of demographic decline in the countries of the Danube Region on the key labour market variables and to model their behaviour. Also, the purpose of this paper is to analyse and discuss the possible consequences of demographic decline and the roles of migration and brain drain in the region. The main conclusion is that drop in the population growth is compensated with migration flows and prolongation of working life when it comes to the active labour force in the Danube Region, although population is still a main source of working force.
Knowledge and Attitudes about Green Consumption in Bulgaria
Authors: Stoimenova Borislava 
Published: March 2017

Abstract
This paper examines the environmental knowledge of the Bulgarians and its relationship with the environmental concern and the predisposition to compromise as two commonly studied attitudes towards green consumption. A quota sample (quotas for age and gender) of 2018 adults from all the regions in Bulgaria is used. The research tool for face-to-face interviews and online surveys is a structured interview. Descriptive statistics, hypothesis tests for difference of means between two independent samples, the correlation analysis (chi-square test for independence) and the cluster analysis (Kmeans cluster) are the methods applied for the analysis. The results indicate that the Bulgarians are moderately knowledgeable about the environmental problems and the depth of knowledge is moderate as well. The environmental knowledge differs between different demographic groups: the better educated, younger, those living in the cities and wealthier people have better self-reported knowledge about the environmental problems. A large proportion of the adults do not consider themselves informed about the environmental problems and among those who do, the people who find themselves generally informed are majorities. The environmental knowledge positively affects both the environmental concern and the predisposition for green consumption.
The Practice of Employees' Training in Serbia based on Cranet Research
Authors: Berber Nemanja , Slavić Agneš 
Published: March 2017

AbstractIn a contemporary environment which is characterised by global competition and radical economic changes, human capital, with its knowledge, competencies, and experiences, is becoming more and more important factor for gaining sustainable business performances. The aim of this paper is to explore the development and current state of the training practices in human resource management in Serbian organizations. The subject of the research is the employees’ training process. The methodology of the research included the analysis of available literature and the exploration of the empirical data on employees’ training in organizations from Serbia. The analysis is based on the comparison between two research periods in which the authors gathered the data. 160 organisations in 2015 and 50 in 2008 were examined during the Cranet project. The results indicate slight improvements in training practice in Serbia. Also, organisations that invest more in training in Serbia gained higher levels of productivity and service quality.