bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector
Authors: Sano Masayuki , Ohtaka Manami , Iijima Minoru , Nakasu Asako , Kato Yoshio , Nakanishi Mahito 
Published: September 2017

AbstractMicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the post-transcriptional level. Different types of cells express unique sets of miRNAs that can be exploited as potential molecular markers to identify specific cell types. Among the variety of miRNA detection methods, a fluorescence-based imaging system that utilises a fluorescent-reporter gene regulated by a target miRNA offers a major advantage for long-term tracking of the miRNA in living cells. In this study, we developed a novel fluorescence-based miRNA-monitoring system using a non-integrating cytoplasmic RNA vector based on a replication-defective and persistent Sendai virus (SeVdp). Because SeVdp vectors robustly and stably express transgenes, this system enabled sensitive monitoring of miRNAs by fluorescence microscopy. By applying this system for cellular reprogramming, we found that miR-124, but not miR-9, was significantly upregulated during direct neuronal conversion. Additionally, we were able to isolate integration-free human induced pluripotent stem cells by long-term tracking of let-7 expression. Notably, this system was easily expandable to allow detection of multiple miRNAs separately and simultaneously. Our findings provide insight into a powerful tool for evaluating miRNA expression during the cellular reprogramming process and for isolating reprogrammed cells potentially useful for medical applications.
Increasing the detection distance of remote NMR using wireless inductive coupling coil
Authors: Killner Mario Henrique M. , Tosin Giancarlo , Carvalho André S. , Firme Bernardes Diego , Colnago Luiz Alberto 
Published: September 2017

AbstractUnilateral nuclear magnetic resonance (UNMR) spectrometers have been applied in a variety of fields such as petrochemistry, materials science, and process control
1
. In UNMR measurements the sample is placed outside of the UNMR sensor and the signal intensity is reduced almost exponentially as the sample-to-sensor distances increases. To expand the detection limits of remote UNMR sensors, wireless inductive coupling was proposed and tested. This strategy was proved to reduce signal attenuation due to sample detachment from sensor, resulting in an increase in detection distance by one order of magnitude (i.e., from few millimeters to few centimeters). This novel approach broadens the potential applications of UNMR sensors and opens new opportunities in several areas, from chemical to biomedical applications.
MicroRNA-134 regulates poliovirus replication by IRES targeting
Authors: Bakre Abhijeet A. , Shim Byoung-Shik , Tripp Ralph A. 
Published: September 2017

AbstractGlobal poliovirus eradication efforts include high vaccination coverage with live oral polio vaccine (OPV), surveillance for acute flaccid paralysis, and OPV “mop-up” campaigns. An important objective involves host-directed strategies to reduce PV replication to diminish viral shedding in OPV recipients. In this study, we show that microRNA-134-5p (miR-134) can regulate Sabin-1 replication but not Sabin-2 or Sabin-3 via direct interaction with the PV 5′UTR. Hypochromicity data showed miR-134 binding to Sabin-1 and 3 but not Sabin-2 IRES. Transfection of a miR-134 mimic repressed translation of Sabin-1 5′UTR driven luciferase validating the mechanism of miR-134-mediated repression of Sabin-1. Further, site directed mutagenesis of the miR-134 binding site in Sabin-1 IRES relieved miR-134-mediated repression indicating that these regulatory molecules have an important role in regulating the host gene response to PV. Binding of miR-134 to Sabin-1 IRES caused degradation of the IRES transcript in a miR-134 and sequence specific manner. The miR-134 binding site was found to be highly conserved in wild type PV-1 as well as EV71 strains indicating that miR-134 may regulate function of these IRES sequences in circulation.
Low-dose YC-1 combined with glucose and insulin selectively induces apoptosis in hypoxic gastric carcinoma cells by inhibiting anaerobic glycolysis
Authors: Wakiyama Kota , Kitajima Yoshihiko , Tanaka Tomokazu , Kaneki Masao , Yanagihara Kazuyoshi , Aishima Shinichi , Nakamura Jun , Noshiro Hirokazu 
Published: September 2017

AbstractThis study aimed to establish a therapeutic strategy targeting hypoxic cancer cells in gastric carcinoma (GC). YC-1 is a HIF-1α inhibitor, and we revealed that low-dose YC-1 (10 µM) suppressed HIF-1α expression, and induced hypoxia-dependent apoptosis in the GC cell line 58As9. This hypoxia-specific apoptosis induction by YC-1 involved excessive reactive oxygen species (ROS) generation. The apoptotic effect of 10 µM YC-1 was enhanced by additional glucose (G) and insulin (I) treatments. RT-PCR demonstrated that 10 µM YC-1 reduced hypoxia-induced expression of HIF-1α targets involved in anaerobic glycolysis. Metabolic analysis showed that YC-1 shifted glucose metabolism in hypoxic cells from anaerobic glycolysis to oxidative phosphorylation (OXPHOS). Additional GI accelerated membranous GLUT1 translocation, elevating glucose uptake, and increased acetyl-CoA levels, leading to more ROS generation in hypoxic YC-1-treated cells. Finally, we evaluated the anti-cancer effect of low-dose YC-1 (1 mg/kg) + G (2 g/kg) and I (1 unit/3 g G) treatment in xenograft models. YC-1 + GI therapy strongly inhibited tumour growth. Immunohistochemical analysis demonstrated that YC-1 + GI reduced HIF-1α expression and pimonidazole accumulation in tumours. Conversely, YC-1 + GI increased intra-tumoral 8-OHdG and levels of apoptosis markers. Low-dose YC-1 + GI is a unique therapy targeting hypoxic GC cells that generates lethal ROS via forced activation of OXPHOS.
Temporal transcriptomic profiling of the ant-feeding assassin bug Acanthaspis cincticrus reveals a biased expression of genes associated with predation in nymphs
Authors: Kou Fei , Li Hu , Li Shujuan , Xun Huaizhu , Zhang Yinqiao , Sun Ziqiang , Zhou Xuguo , Cai Wanzhi 
Published: September 2017

AbstractAcanthaspis cincticrus (Stål) is an assassin bug with a specialized camouflaging behavior to ambush ants in the nymphal stages. In this study, we comprehensively sequenced all the life stages of A. cincticrus, including the eggs, five nymph instars, female and male adults using Illumina HiSeq technology. We obtained 176 million clean sequence reads. The assembled 84,055 unigenes were annotated and classified functionally based on protein databases. Among the unigenes, 29.03% were annotated by one or more databases, suggesting their well-conserved functions. Comparison of the gene expression profiles in the egg, nymph and adult stages revealed certain bias. Functional enrichment analysis of significantly differentially expressed genes (SDEGs) showed positive correlation with specific physiological processes within each stage, including venom, aggression, olfactory recognition as well as growth and development. Relative expression of ten SDEGs involved in predation process was validated using quantitative real-time PCR (qRT-PCR).
The influence of liposomal quercetin on liver damage induced by microwave ablation
Authors: Duan Xuhua , Chen Pengfei , Han Xinwei , Ren Jianzhuang , Wang Zhaoyang , Zhao Guorui , Li Hao 
Published: September 2017

AbstractThis study aimed to observe whether liposomal quercetin (LQ) can enhance the effect of microwave ablation (MWA) on hepatic parenchyma destruction. Forty-eight rabbits were randomly divided into three groups: LQ group, MWA group and LQ + MWA group. Serum and liver samples were collected. The coagulation volume (CV) of hepatic parenchyma, histopathological changes and liver function were compared. Hepatocyte apoptosis was examined through TUNEL. The expression of heat shock protein 70 (HSP70), hypoxia-inducible factor-1α (HIF-1α) and tumor necrosis factor-α (TNF-α) were analyzed. Compared with MWA group, the CV of coagulation necrosis in liver was significantly increased in LQ + MWA group. TUNEL results showed that the hepaocyte apoptosis was higher in LQ + MWA group than MWA group on 12 h, 24 h and 3 d, respectively. HSP70 and HIF-1α expression in both MWA group and LQ + MWA group were increased at 12 and 24 hours, peaked on day3 and dropped on day7. Compared with MWA group, HSP70 and HIF-1α expression were lower in LQ + MWA group. On the contrary, TNF-α expression was decreased in MWA group and LQ + MWA group compared with LQ group. In conclusion, LQ increased hepatocyte apoptosis and MWA-induced hepatic parenchyma destruction through suppressing HSP70 and HIF-1α expression in liver surrounding ablation zone and increasing TNF-α expression.
Association of Plasma Magnesium with Prediabetes and Type 2 Diabetes Mellitus in Adults
Authors: Chen Sijing , Jin Xiaoling , Liu Jun , Sun Taoping , Xie Manling , Bao Wei , Yu Xuefeng , Yang Xuefeng , Zhang Yan , Zhang Haibo , Shan Zhilei , Liu Liegang 
Published: October 2017

AbstractOur study aimed to assess the associations of plasma magnesium with prediabetes and type 2 diabetes (T2D) among Chinese adults. We conducted a case-control analysis of 4447 participants: 867 newly diagnosed prediabetes patients, 1475 newly diagnosed T2D patients and 2105 normal glucose tolerance (NGT) individuals. In a subsample of 599 apparently healthy individuals, we measured plasma hs-CRP levels to examine their relation to plasma magnesium. Plasma magnesium and hs-CRP were measured by inductively coupled plasma mass spectrometry and an enzyme-linked immunosorbent assay, respectively. Plasma magnesium decreased from NGT to prediabetes to T2D, and was inversely associated with prediabetes and T2D. After adjustment for potential confounding factors, the ORs from the lowest to highest quartiles of plasma magnesium were 1, 0.57 (95%CI, 0.44–0.74), 0.49 (0.37–0.65) and 0.51 (0.37–0.70) for prediabetes, and 1, 0.26 (0.20–0.33), 0.15 (0.12–0.20) and 0.15 (0.11–0.20) for T2D. Consistently, plasma magnesium was inversely correlated with plasma hs-CRP in our subsample analysis; the geometric mean hs-CRP concentration for ascending quartiles of plasma magnesium were 1.29 (1.06–1.57), 1.16 (0.95–1.41), 1.00 (0.81–1.22), and 0.71 (0.58–0.88) mg/l. Plasma magnesium was independently and inversely associated with prediabetes and T2D in Chinese adults.
Reconfigurable photonic dual‐frequency phase‐coded signals generator by using optical carrier phase shifting and polarisation selection
Authors: Li Xuan , Zhao Shanghong , Zhu Zihang , Qu Kun , Lin Tao , Hu Dapeng 
Published: September 2017

A novel photonic dual‐frequency phase‐coded signals generator based on the optical carrier phase shifting and polarisation selection techniques is proposed by using an integrated dual‐polarisation quadrature phase shift keying (DP‐QPSK) modulator. The key feature of the scheme is the capability to generate dual‐frequency phase‐coded signals with reconfigurable frequency multiplication factors. In the upper QPSK modulator, an optical binary phase shift keying (BPSK) signal and a carrier‐suppressed double‐sideband optical signal are generated, while in the bottom QPSK modulator, another carrier‐suppressed double‐sideband optical signal is generated. Thanks to the dual‐polarisation property of the integrated modulator, the optical BPSK signal and different optical sidebands can be selected by using polarisation selection. As a result, two phase‐coded signals with different frequencies can be achieved simultaneously. By adjusting the bias phases of the submodulators, dual‐frequency phase‐coded signals with frequencies of one and three, or two and four times the frequency of the RF carrier can be generated. The generation of 2.5 Gbps dual‐frequency phase‐coded signals with frequencies of 20 and 40 GHz, or 10 and 30 GHz are verified. The proposed scheme is simple, compact, and promising to find applications in future multi‐band and multi‐functional radars.
Automated reliability assessment for spectroscopic redshift measurements
Authors: Jamal S. , Le Brun V. , Le Fèvre O. , Vibert D. , Schmitt A. , Surace C. , Copin Y. , Garilli B. , Moresco M. , Pozzetti L. 
Published: September 2017

Context.Future large-scale surveys, such as the ESAEuclidmission, will produce a large set of galaxy redshifts (≥106) that will require fully automated data-processing pipelines to analyze the data, extract crucial information and ensure that all requirements are met. A fundamental element in these pipelines is to associate to each galaxy redshift measurement a quality, or reliability, estimate.Aim.In this work, we introduce a new approach to automate the spectroscopic redshift reliability assessment based on machine learning (ML) and characteristics of the redshift probability density function.Methods.We propose to rephrase the spectroscopic redshift estimation into a Bayesian framework, in order to incorporate all sources of information and uncertainties related to the redshift estimation process and produce a redshift posterior probability density function (PDF). To automate the assessment of a reliability flag, we exploit key features in the redshift posterior PDF and machine learning algorithms.Results.As a working example, public data from the VIMOS VLT Deep Survey is exploited to present and test this new methodology. We first tried to reproduce the existing reliability flags using supervised classification in order to describe different types of redshift PDFs, but due to the subjective definition of these flags (classification accuracy ~58%), we soon opted for a new homogeneous partitioning of the data into distinct clusters via unsupervised classification. After assessing the accuracy of the new clusters via resubstitution and test predictions (classification accuracy ~98%), we projected unlabeled data from preliminary mock simulations for theEuclidspace mission into this mapping to predict their redshift reliability labels.Conclusions.Through the development of a methodology in which a system can build its own experience to assess the quality of a parameter, we are able to set a preliminary basis of an automated reliability assessment for spectroscopic redshift measurements. This newly-defined method is very promising for next-generation large spectroscopic surveys from the ground and in space, such asEuclidand WFIRST.
Close Examination of the Bar Removal Procedure: The Surgeons' Voice
Authors: Ardigo Laura , Bellia-Munzon Gaston , Martinez-Ferro Marcelo , Alvarez-Garcia Natalia 
Published: September 2017

Introduction Complications of the Nuss procedure are well known. However, publications about intraoperative and postoperative complications of the bar removal procedure (BRP) are scarce. Are they uncommon, unknown, or underreported? Are we ready to face them?
Objective This study aims to explore the profile of complications of the BRP, risk perception, and preventive actions undertaken by the Chest Wall International Group (CWIG) surgeons.
Materials and Methods A 14-question online survey was posted to the members of the CWIG from February 1 to 28, 2016. A qualifying question was setup to ensure respondents performed Nuss' procedure/variations. Overall, 96.5% of respondents (n = 116) qualified.
Results Despite being experts in the field of chest wall surgery 62.5% of respondents had performed ≤ 100 BRP and only 37.5% ≥ 100 BRP. Intraoperative complications: Severe bleeding from the bar tunnel was experienced at least one time by 28% of respondents and other serious complications, even death, were reported by 12%. Major surgical maneuvers to treat complications (sternotomy, thoracotomy, life-support maneuvers) were implemented by 62% of the surgeons who had experienced acute complications. Postoperative complications: Overall they were experienced at least once by 73.5% of surgeons. Wound seroma was the most cited complication (42%), followed by pneumothorax (27.5%). Surprisingly, one in every four surgeons acknowledged not requesting a routine chest X-ray before patient discharge. Complications versus experience: The report of acute complications increased with cumulative experience: Close to 50% for surgeons with < 100 BRP and as high as 100% for surgeons with > 1,000 BRP. Prevention of complications: Although 64% of surgeons defined the BRP as of intermediate/high risk, most of them (61%) do not prepare the operating room accordingly (availability of blood products, cardiac surgical backup, and perfusionist support).
Conclusion BRP complications are being underestimated and underreported. Publications do not reflect the real spectrum of complications, which includes life-threatening conditions, even death. Preoperative planning and preparation of the operating room should not be disregarded.
Trimodale Therapie des NSCLC im Stadium IIIA-B mittels neoadjuvanter Hochdosisradiochemotherapie. Stellt die Operation eine sinnvolle Option dar?
Authors: Koryllos Aris , Ludwig Corinna , Engel-Riedel Walburga , Hammer-Helmig Michaela , Stoelben Erich 
Published: September 2017

Zusammenfassung
Einführung Nicht kleinzellige Lungenkarzinome (engl. non-small cell lung cancer = NSCLC) im Stadium III und ihre mögliche multimodale Therapie stellen eine Herausforderung für die behandelnden Onkologen, Thoraxchirurgen und Strahlentherapeuten dar. Ziel dieser retrospektiven Studie war die Analyse und Evaluation des Therapiealgorithmus unserer Klinik bei Patienten mit NSCLC im Stadium III (intention to treat). Es sollte ermittelt werden, ob sich ein aggressives Therapieregime mit primärer trimodaler Therapie (Hochdosisradiochemotherapie und Resektion) unabhängig von einem „multilevel“-N2- oder „single-level“-N3-Status: Diese Resultate wurden anschließend mit einem historischen Kollektiv unserer Patienten verglichen, die eine alleinige simultane Radio-/Chemotherapie erhielten (= bimodale Therapie).
Material und Methoden 156 Patienten wurden in dem Erhebungszeitraum mit einem NSCLC Stadium III diagnostiziert und einer trimodalen Therapie zugeführt. Das mediane Alter war 71 Jahre, 103 Patienten (60%) waren männlich, 53 (34%) waren weiblich. In der Gruppe mit bimodaler Therapie konnten 102 Patienten ausgewertet werden.
Ergebnisse Nach radiologischem Restaging und Überprüfung der funktionellen Resektabilität konnten 90 Patienten (57,7%) aus der trimodalen Therapiegruppe einer sekundären Resektion zugeführt werden. Hierbei wurden 37 (41,1%) Lobektomien/Bilobektomien, 37 (41,1%) Sleeve-Lobektomien, 13 (14,4%) Pneumonektomien und 3 (3,3%) Segmentektomien (bei schwerer lungenfunktioneller Einschränkung) durchgeführt. Das mediane Überleben in der trimodalen Therapiegruppe betrug 535 Tage, in der bimodalen Therapiegruppe hingegen 388 Tage, der Unterschied war statistisch nicht signifikant (p = 0,1377). Abschließend wurde eine Analyse der 5-Jahres-Überlebensrate nach tatsächlicher Therapie durchgeführt („as-treated trimodally“ vs. „as-treated bimodally“). Hier betrug das mediane Überleben 807 Tage für Patienten, die eine trimodale Therapie erhielten, 427 Tage für Patienten die eine bimodale Therapie erhielten.
Schlussfolgerung Eine hochdosierte neoadjuvante Radiochemotherapie gefolgt von einer sekundären Resektion stellt weiterhin eine wertvolle Option für selektionierte Patienten in der Behandlung des NSCLC im Stadium III dar. Diese retrospektive Analyse konnte jedoch einen statistisch signifikanten Überlebensvorteil für die „intention-to-treat-trimodally“-Patienten nicht nachweisen.
Die Bedeutung der Chirurgie beim oligometastatischen und oligoprogressiven nicht kleinzelligen Lungenkarzinom
Authors: Schmid Severin , Passlick Bernward 
Published: September 2017

ZusammenfassungDas bessere Verständnis der Tumorbiologie sowie die neuen Therapien beim nicht kleinzelligen Lungenkarzinom haben zu einer weiteren Differenzierung der Therapie auch der metastasierten Erkrankung geführt. Der Begriff des oligometastatischen Lungenkarzinoms beschreibt vergleichsweise gutartige Verläufe mit nur wenigen, häufig auf ein Organsystem beschränkten Metastasen. Zum Erreichen eines optimalen Behandlungsergebnisses für jede charakteristische Ausprägung bzw. Tumorbiologie muss ein adäquater Therapieansatz unter Einbeziehung der verschiedenen Therapiemodalitäten gefunden werden. In diesem Rahmen gewinnt die Chirurgie auch in kurativen Konzepten zunehmend an Bedeutung. Analog zum oligometastatischen Lungenkarzinom wurde der Begriff der oligoprogressiven Erkrankung geprägt. Dieser resultierte insbesondere aus den Beobachtungen des Krankheitsverlaufs bei Lungenkarzinomen, die eine der gängigen Treibermutationen, wie die ALK-Fusion oder die EGFR-Mutation, aufweisen. In einigen Fällen findet sich hier eine auf wenige Herde begrenzte Progression der metastasierten Erkrankung bei sonst stabilem Befund. Bisherige Studien zeigen sowohl beim oligometastatischen als auch beim oligoprogressiven Lungenkarzinom bei entsprechend selektionierten Patienten ermutigende Ergebnisse, mit z. T. deutlichem Überlebensvorteil durch ein aggressives chirurgisches Vorgehen in Kombination mit der üblicherweise durchgeführten Systemtherapie. Zu klären bleibt jedoch, ob der beobachtete Überlebensvorteil lediglich aufgrund der günstigeren Tumorbiologie bei den ausgewählten Patienten oder aber aufgrund der gewählten Therapien zustande kommt. Zudem bedarf es in Zukunft exakterer Marker zur Identifikation geeigneter Patientenkollektive.
Aufklärung bei Ästhetischen Operationen – was man wissen
muss
Authors: Bichler Christian , Würfel Martin 
Published: September 2017

ZusammenfassungBesondere Bedeutung gewinnt eine ordnungsgemäße Aufklärung vor dem Hintergrund,
dass nahezu jeder ärztliche Eingriff eine strafrechtlich relevante
Körperverletzung darstellt, die der Rechtfertigung bedarf. Daher muss der
Patient zunächst darüber informiert werden, worin er überhaupt einwilligt. Bei
nicht medizinisch indizierten plastischen Eingriffe ist eine besonders intensive
Aufklärung erforderlich. Man spricht hierbei von einer „schonungslosen“
Aufklärung.
Prävalenz, Ursachen und Therapiemöglichkeiten von pathologischen Kiefergelenkbefunden im Kindes- und Erwachsenenalter – ein Update
Authors: Jung Britta , Heekeren Daniel , Tornier Alexandra 
Published: September 2017

ZusammenfassungPathologische Kiefergelenkbefunde im Sinne einer kraniomandibulären Dysfunktion (CMD) können grundsätzlich in jedem Lebensalter auftreten. Aus dem CMD-Spektrum greift der Beitrag Schwerpunkte heraus, beleuchtet Häufigkeit und mögliche Ursachen und zeigt befundabhängig verschiedene Möglichkeiten und Apparaturen zur Therapie.Der vorliegende Artikel basiert auf dem wissenschaftlichen Beitrag „Pathologische Kiefergelenksbefunde im Kindes- und Jugendalter“ aus dem Jahr 2015 1. Er hat das Ziel Prävalenzen und Ursachen nicht nur im Kindesalter, sondern auch im Erwachsenenalter sowie diagnostische und therapeutische Möglichkeiten vor dem Hintergrund aktueller Literaturdaten aufzuzeigen.