bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of the Plant Growth-Promoting Bacterium
Pseudomonas
sp. SCPG-7, Isolated from Saline Soil
Authors: Han Yajie , Dai Bin , Zhou Ying , Wu Zhansheng , Ye Bang-Ce 
Published: August 2017

ABSTRACT

Pseudomonas
sp. SCPG-7 was isolated from saline soil. The strain can increase the germination rate of cotton seeds and promote the growth of cotton seedlings under salt stress conditions. The genome is 6,256,198 bp long, containing 5,672 predicted open reading frames.
Draft Genome Sequence of
Methylocaldum
sp. SAD2, a Methanotrophic Strain That Can Convert Raw Biogas to Methanol in the Presence of Hydrogen Sulfide
Authors: Wei Xiangdong , Ge Xumen , Li Yebo , Yu Zhongtang 
Published: August 2017

ABSTRACT

The draft genome sequence of
Methylocaldum
sp. SAD2, a methanotrophic strain isolated from a hydrogen sulfide-rich anaerobic digester, is reported here. Strain SAD2 possesses genes for methane oxidation in the presence of H
2
S.
Whole-Genome Sequences of Two Carbapenem-Resistant Klebsiella quasipneumoniae Strains Isolated from a Tertiary Hospital in Johor, Malaysia
Authors: Gan Han Ming , Rajasekaram Ganeswrie , Eng Wilhelm Wei Han , Kaniappan Priyatharisni , Dhanoa Amreeta 
Published: August 2017

ABSTRACT

We report the whole-genome sequences of two carbapenem-resistant clinical isolates of
Klebsiella quasipneumoniae
subsp.
similipneumoniae
obtained from two different patients. Both strains contained three different extended-spectrum β-lactamase genes and showed strikingly high pairwise average nucleotide identity of 99.99% despite being isolated 3 years apart from the same hospital.
Why don’t serum vitamin D concentrations associate with BMD by DXA? A case of being ‘bound’ to the wrong assay? Implications for vitamin D screening
Authors: Allison Richard J , Farooq Abdulaziz , Cherif Anissa , Hamilton Bruce , Close Graeme L , Wilson Mathew G 
Published: August 2017

BackgroundThe association between bone mineral density (BMD) and serum25-hydroxyvitamin D (25(OH)D) concentration is weak, particularly in certain races (eg, BlackAfrican vs Caucasian) and in athletic populations. We aimed to examine if bioavailable vitamin D rather than serum 25(OH)D was related to markers of bone health within a racially diverse athletic population.MethodsIn 604 male athletes (Arab (n=327), Asian (n=48), Black (n=108), Caucasian (n=53) and Hispanic (n=68)), we measured total 25(OH)D, vitamin D-binding protein and BMD by DXA. Bioavailable vitamin D was calculated using the free hormone hypothesis.ResultsFrom 604 athletes, 21.5% (n=130) demonstrated severe 25(OH)D deficiency, 37.1% (n=224) deficiency, 26% (n=157) insufficiency and 15.4% (n=93) sufficiency. Serum 25(OH)D concentrations were not associated with BMD at any site. After adjusting for age and race, bioavailable vitamin D was associated with BMD (spine, neck and hip). Mean serum vitamin D binding protein concentrations were not associated with 25(OH)D concentrations (p=0.392).ConclusionRegardless of age or race, bioavailable vitamin D and not serum 25(OH)D was associated with BMD in a racially diverse athletic population. If vitamin D screening is warranted, clinicians should use appropriate assays to calculate vitamin D binding protein and bioavailable vitamin D levels concentrations than serum 25(OH)D. In turn, prophylactic vitamin D supplementation to ‘correct’ insufficient athletes should not be based on serum 25(OH)D measures.
Establishing the aims, format and function for multidisciplinary team-driven care within an inflammatory bowel disease service: a multicentre qualitative specialist-based consensus study
Authors: Morar Pritesh S , Sevdalis Nick , Warusavitarne Janindra , Hart Ailsa , Green James , Edwards Cathryn , Faiz Omar 
Published: August 2017

ObjectiveTo obtain a specialist-based consensus on the aims, format and function for MDT-driven care within an inflammatory bowel disease (IBD) service.DesignThis was a prospective, multicentre study using a Delphi formal consensus-building methodology.SettingParticipants were recruited nationally across 13 centres from July to August 2014.Participants24 participants were included into the Delphi Specialist Consensus Panel. They included six consultant colorectal surgeons, six gastroenterologists, five consultant radiologists, three consultant histopathologists and 4 IBD nurse specialists.InterventionsPanellists ranked items on a Likert scale (1=not important to 5=very important). Items with a median score >3 were considered eligible for inclusion.Main outcome measuresConsensus was defined with an IQR ≤1. Consensus on categorical responses was defined by an agreement of >60%.ResultsA consensus on items (median; IQR) that described the aims of the MDT-driven care that were consideredvery importantincluded:advance patient care(5;5-5),provide multidisciplinary input for the patient’s care plan(5;5-5),provide shared experience and expertise(5;5-5),improve patient outcome(5;5-5),deliver the best possible care for the patient(5;5-5) andto obtain consensus on management for a patient with IBD(5;4-5). A consensus for being a core MDT member was demonstrated for colorectal surgeons (24/24), radiologists (24/24), gastroenterologists (24/24), nurse specialists (24/24), dieticians (14/23), histopathologists (21/23) and coordinators (21/24).ConclusionsThis study has provided a consensus for proposed aims, overall design, format and function MDT-driven care within an IBD service. This can provide a focus for core members, and aid a contractual recognition to ensure attendance and proactive contribution.
Coronary angiography in worsening heart failure: determinants, findings and prognostic implications
Authors: Ferreira João Pedro , Rossignol Patrick , Demissei Biniyam , Sharma Abhinav , Girerd Nicolas , Anker Stefan D , Cleland John G , Dickstein Kenneth , Filippatos Gerasimos , Hillege Hans L , Lang Chim C , Metra Marco , Ng Leong L , Ponikowski Piotr , Samani Nilesh J , van Veldhuisen Dirk J , Zwinderman Aeilko H , Voors Adriaan , Zannad Faiez 
Published: August 2017

ObjectivesCoronary angiography is regularly performed in patients with worsening signs and/or symptoms of heart failure (HF). However, little is known on the determinants, findings and associated clinical outcomes of coronary angiography performed in patients with worsening HF.MethodsThe BIOSTAT-CHF (a systems BIOlogy Study to TAilored Treatment in Chronic Heart Failure) programme enrolled 2516 patients with worsening symptoms and/or signs of HF, either hospitalised or in the outpatient setting. All patients were included in the present analysis.ResultsOf the 2516 patients included, 315 (12.5%) underwent coronary angiography within the 30 days after the onset of worsening symptoms and/or signs of HF. Subjects who underwent angiography were more often observed as inpatients, had more often an overt acute coronary syndrome, had higher troponin I levels, were younger and had better renal function (all p≤0.01). Patients who underwent coronary angiography had a lower risk of the primary outcome of death and/or HF hospitalisation (adjusted HR=0.71, 95% CI 0.57 to 0.89, p=0.003) and death (adjusted HR=0.59, 95% CI 0.43 to 0.80, p=0.001). Among the patients who underwent coronary angiography, those with a coronary stenosis (39%) had a worse prognosis than those without stenosis (adjusted HR for the primary outcome=1.71, 95% CI 1.10 to 2.64, p=0.016).ConclusionsCoronary angiography was performed in <13% of patients with symptoms and/or signs of worsening HF. These patients were remarkably different from those who did not undergo coronary angiography and had a lower risk of subsequent events. The presence of coronary stenosis on coronary angiography was associated with a worse prognosis.
Thrombectomy of calcified emboli in stroke. Does histology of thrombi influence the effectiveness of thrombectomy?
Authors: Dobrocky Tomas , Piechowiak Eike , Cianfoni Alessandro , Zibold Felix , Roccatagliata Luca , Mosimann Pascal , Jung Simon , Fischer Urs , Mordasini Pasquale , Gralla Jan 
Published: August 2017

Background and purposeThrombus composition has been postulated to affect the success of endovascular therapy. Calcified clots are composed of large amounts of calcium phosphate which influences their mechanical properties and may serve as a model for testing this hypothesis. The aim of this study was to evaluate the recanalization and complication rates of calcified thromboemboli in patients with acute ischemic stroke who underwent thrombectomy.Material and methodsA retrospective analysis was performed of all calcified intracranial thromboemboli in patients suffering an acute ischemic stroke, referred for endovascular therapy at two centers between January 2013 and July 2016.ResultsEight patients with a calcified intracranial clot underwent stent retriever thrombectomy (five women; mean age 80 years). Mean clot attenuation was 305 HU (range 150–640 HU). Successful reperfusion defined, as Thrombolysis in Cerebral Infarction grade 2b–3 was achieved in only one patient (12.5%). Two periprocedural adverse events occurred: one peripheral vessel perforation which was coiled and one inadvertent stent retriever detachment due to fracture of the stent retriever wire.ConclusionStent retriever thrombectomy of calcified thromboemboli seems less effective than with other types of clots. Different mechanical properties of calcified clots may render them stiffer and less accessible for stent retrievers. When faced with a calcified intracranial thromboembolus in clinical practice, a more contained approach may be warranted in view of low recanalization rates, and the potential for periprocedural adverse events.
Peripheral ventilation heterogeneity determines the extent of bronchoconstriction in asthma
Authors: Farrow Catherine E. , Salome Cheryl M. , Harris Benjamin E. , Bailey Dale L. , Berend Norbert , King Gregory G. 
Published: August 2017

In asthma, bronchoconstriction causes topographically heterogeneous airway narrowing, as measured by three-dimensional ventilation imaging. Computation modeling suggests that peripheral airway dysfunction is a potential determinant of acute airway narrowing measured by imaging. We hypothesized that the development of low-ventilation regions measured topographically by three-dimensional imaging after bronchoconstriction is predicted by peripheral airway function. Fourteen asthmatic subjects underwent ventilation single-photon-emission computed tomography/computed tomography scan imaging before and after methacholine challenge. One-liter breaths of Technegas were inhaled from functional residual capacity in upright posture before supine scanning. The lung regions with the lowest ventilation (Ventlow) were calculated using a thresholding method and expressed as a percentage of total ventilation (Venttotal). Multiple-breath nitrogen washout was used to measure diffusion-dependent and convection-dependent ventilation heterogeneity (Sacin and Scond, respectively) and lung clearance index (LCI), before and after challenge. Forced expiratory volume in 1 s (FEV1) was 87.6 ± 15.8% predicted, and seven subjects had airway hyperresponsiveness. Ventlowat baseline was unrelated to spirometry or multiple-breath nitrogen washout indices. Methacholine challenge decreased FEV1by 23 ± 5% of baseline while Ventlowincreased from 21.5 ± 2.3%Venttotalto 26.3 ± 6.7%Venttotal( P = 0.03). The change in Ventlowwas predicted by baseline Sacin ( rs = 0.60, P = 0.03) and by LCI ( rs = 0.70, P = 0.006) but not by Scond ( rs = 0.30, P = 0.30). The development of low-ventilation lung units in three-dimensional ventilation imaging is predicted by ventilation heterogeneity in diffusion-dependent airways. This relationship suggests that acinar ventilation heterogeneity in asthma may be of mechanistic importance in terms of bronchoconstriction and airway narrowing.NEW & NOTEWORTHY Using ventilation SPECT/CT imaging in asthmatics, we show induced bronchoconstriction leads to the development of areas of low ventilation. Furthermore, the relative volume of the low-ventilation regions was predicted by ventilation heterogeneity in diffusion-dependent acinar airways. This suggests that the pattern of regional airway narrowing in asthma is determined by acinar airway function.
The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling
Authors: Jourd’heuil Frances L. , Xu Haiyan , Reilly Timothy , McKellar Keneta , El Alaoui Chaymae , Steppich Julia , Liu Yong Feng , Zhao Wen , Ginnan Roman , Conti David , Lopez-Soler Reynold , Asif Arif , Keller Rebecca K. , Schwarz John J. , Thanh Thuy Le Thi , Kawada Norifumi , Long Xiaochun , Singer Harold A. , Jourd’heuil David 
Published: August 2017

Objective—
The role of hemoglobin and myoglobin in the cardiovascular system is well established, yet other globins in this context are poorly characterized. Here, we examined the expression and function of cytoglobin (CYGB) during vascular injury.

Approach and Results—

We characterized CYGB content in intact vessels and primary vascular smooth muscle (VSM) cells and used 2 different vascular injury models to examine the functional significance of CYGB in vivo. We found that CYGB was strongly expressed in medial arterial VSM and human veins. In vitro and in vivo studies indicated that CYGB was lost after VSM cell dedifferentiation. In the rat balloon angioplasty model, site-targeted delivery of adenovirus encoding shRNA specific for CYGB prevented its reexpression and decreased neointima formation. Similarly, 4 weeks after complete ligation of the left common carotid,
Cygb
knockout mice displayed little to no evidence of neointimal hyperplasia in contrast to their wild-type littermates. Mechanistic studies in the rat indicated that this was primarily associated with increased medial cell loss, terminal uridine nick-end labeling staining, and caspase-3 activation, all indicative of prolonged apoptosis. In vitro, CYGB could be reexpressed after VSM stimulation with cytokines and hypoxia and loss of CYGB sensitized human and rat aortic VSM cells to apoptosis. This was reversed after antioxidant treatment or NOS2 (nitric oxide synthase 2) inhibition.

Conclusions—
These results indicate that CYGB is expressed in vessels primarily in differentiated medial VSM cells where it regulates neointima formation and inhibits apoptosis after injury.
Novel Genetic Triggers and Genotype–Phenotype Correlations in Patients With Left Ventricular Noncompaction
Authors: Miszalski-Jamka Karol , Jefferies John L. , Mazur Wojciech , Głowacki Jan , Hu Jianhong , Lazar Monika , Gibbs Richard A. , Liczko Jacek , Kłyś Jan , Venner Eric , Muzny Donna M. , Rycaj Jarosław , Białkowski Jacek , Kluczewska Ewa , Kalarus Zbigniew , Jhangiani Shalini , Al-Khalidi Hussein , Kukulski Tomasz , Lupski James R. , Craigen William J. , Bainbridge Matthew N. 
Published: August 2017

Background—
Left ventricular noncompaction (LVNC) is a genetically and phenotypically heterogeneous disease and, although increasingly recognized in clinical practice, there is a lack of widely accepted diagnostic criteria. We sought to identify novel genetic causes of LVNC and describe genotype–phenotype correlations.

Methods and Results—

A total of 190 patients from 174 families with left ventricular hypertrabeculation (LVHT) or LVNC were referred for cardiac magnetic resonance and whole-exome sequencing. A total of 425 control individuals were included to identify variants of interest (VOIs). We found an excess of 138 VOIs in 102 (59%) unrelated patients in 54 previously identified LVNC or other known cardiomyopathy genes. VOIs were found in 68 of 90 probands with LVNC and 34 of 84 probands with LVHT (76% and 40%, respectively;
P
<0.001). We identified 0, 1, and ≥2 VOIs in 72, 74, and 28 probands, respectively. We found increasing number of VOIs in a patient strongly correlated with several markers of disease severity, including ratio of noncompacted to compacted myocardium (
P
<0.001) and left ventricular ejection fraction (
P
=0.01). The presence of sarcomeric gene mutations was associated with increased occurrence of late gadolinium enhancement (
P
=0.004).

Conclusions—
LVHT and LVNC likely represent a continuum of genotypic disease with differences in severity and variable phenotype explained, in part, by the number of VOIs and whether mutations are present in sarcomeric or nonsarcomeric genes. Presence of VOIs is common in patients with LVHT. Our findings expand the current clinical and genetic diagnostic approaches for patients with LVHT and LVNC.
CMR Native T1 Mapping Allows Differentiation of Reversible Versus Irreversible Myocardial Damage in ST-Segment–Elevation Myocardial Infarction
Authors: Liu Dan , Borlotti Alessandra , Viliani Dafne , Jerosch-Herold Michael , Alkhalil Mohammad , De Maria Giovanni Luigi , Fahrni Gregor , Dawkins Sam , Wijesurendra Rohan , Francis Jane , Ferreira Vanessa , Piechnik Stefan , Robson Matthew D. , Banning Adrian , Choudhury Robin , Neubauer Stefan , Channon Keith , Kharbanda Rajesh , Dall’Armellina Erica 
Published: August 2017

Background—
CMR T1 mapping is a quantitative imaging technique allowing the assessment of myocardial injury early after ST-segment–elevation myocardial infarction. We sought to investigate the ability of acute native T1 mapping to differentiate reversible and irreversible myocardial injury and its predictive value for left ventricular remodeling.

Methods and Results—

Sixty ST-segment–elevation myocardial infarction patients underwent acute and 6-month 3T CMR, including cine, T2-weighted (T2W) imaging, native shortened modified look-locker inversion recovery T1 mapping, rest first pass perfusion, and late gadolinium enhancement. T1 cutoff values for oedematous versus necrotic myocardium were identified as 1251 ms and 1400 ms, respectively, with prediction accuracy of 96.7% (95% confidence interval, 82.8% to 99.9%). Using the proposed threshold of 1400 ms, the volume of irreversibly damaged tissue was in good agreement with the 6-month late gadolinium enhancement volume (
r
=0.99) and correlated strongly with the log area under the curve troponin (
r
=0.80) and strongly with 6-month ejection fraction (
r
=−0.73). Acute T1 values were a strong predictor of 6-month wall thickening compared with late gadolinium enhancement.

Conclusions—
Acute native shortened modified look-locker inversion recovery T1 mapping differentiates reversible and irreversible myocardial injury, and it is a strong predictor of left ventricular remodeling in ST-segment–elevation myocardial infarction. A single CMR acquisition of native T1 mapping could potentially represent a fast, safe, and accurate method for early stratification of acute patients in need of more aggressive treatment. Further confirmatory studies will be needed.
Stability of the Tropical Cyclone Intensity Equilibrium
Authors: Kieu Chanh , Wang Quan 
Published: August 2017

Abstract
A low-order dynamical model that demonstrates the stable property of the tropical cyclone (TC) maximum potential intensity (MPI) equilibrium under the wind-induced surface heat exchange feedback was recently presented by Kieu. In this study, an alternative TC-scale model that allows for the gradient wind imbalance in the planetary boundary layer is proposed to further examine the stability of the MPI equilibrium. Despite different balanced assumptions, it is shown that the new TC-scale model possesses a similar MPI equilibrium with the same asymptotic stability. The structurally stable property of the MPI equilibrium is held for a range of the model parameters, regardless of the model initial conditions or numerical configurations. In addition, an explicit dependence of the MPI on the environmental lapse rate is obtained from the new TC-scale model, which reveals subtle impacts of the tropospheric stratification on TC intensity beyond the traditional MPI framework. The existence and interpretation of two distinct time scales during TC development will be also discussed.
Longitudinal Effects of Personality on Physical Activity Among College Students: Examining Executive Function as a Potential Moderator
Authors: Joyner Chelsea , Loprinzi Paul D. 
Published: August 2017

Objective The purpose of this study was to examine the relationship between personality and physical activity, with a specific focus on whether executive function moderates this relationship. Methods One hundred twenty-six young adults provided complete data at baseline and the five-month follow-up assessment. Executive function was assessed via the parametric Go/No-Go computer task; outcome measures of parametric Go/No-Go were mean reaction time and percent of correct target detection across executive function tasks (simple rule and repeating rule). Personality and moderate-to-vigorous physical activity were assessed via validated questionnaires; one-week test–retest was established on a random sample of the participants, with pedometry employed in the subsample. Results Individuals with a higher baseline conscientiousness personality type had greater five-month follow-up moderate-to-vigorous physical activity (β = 18.5; 95% confidence interval: 5.3, 31.7; p = .006). There was no evidence of an interaction effect for personality trait and executive function on five-month follow-up moderate-to-vigorous physical activity. Conclusion The personality trait conscientiousness was associated with greater moderate-to-vigorous physical activity. However, there was no evidence to suggest that executive function moderates the role between personality and moderate-to-vigorous physical activity. Thus, if confirmed by future work, personality may exert its effects on moderate-to-vigorous physical activity independent of executive function level.
Does Propofol Sedation Contribute to Overall Energy Provision in Mechanically Ventilated Critically Ill Adults? A Retrospective Observational Study
Authors: Hastings Jennifer , Ridley Emma J. , Bianchet Oliver , Roodenburg Owen , Levkovich Bianca , Scheinkestel Carlos , Pilcher David , Udy Andrew 
Published: August 2017

AbstractBackground: Propofol sedation is common in critically ill patients, providing energy of 1.1 kcal/mL when administered as a 1% solution. We aimed to determine the proportion of energy administered as propofol on days 1–5 in the intensive care unit (ICU) and any association with outcomes. Methods: Retrospective observational study in a quaternary ICU from January–December 2012. Inclusion criteria were length of stay (LOS) ≥5 days, age ≥18 years, and provision of mechanical ventilation (MV) for ≥5 days. Outcome measures included proportion of total daily energy provided as propofol, overall energy balance, hospital mortality, duration of MV, and ICU LOS. Results: Data from 370 patients were analyzed, 87.8% (n = 325) of whom received propofol during days 1–5 in ICU. A median [interquartile range (IQR)] of 119 [50–730] kcal was provided as propofol per patient‐day. Proportion of energy provided by propofol as a percentage of total energy delivered was 55.4%, 15.4%, 9.3%, 7.9%, and 9.9% days 1–5, respectively. Patients administered propofol received a greater proportion of their total daily energy prescription compared with those who were not (P < .01). Proportion of energy provided as propofol was not significantly different based on hospital mortality (P = .62), duration of MV (P = .50), or ICU LOS (P = .15). Conclusion: Propofol contributes to overall energy intake on days 1–5 of ICU admission. Energy balance was higher in those receiving propofol. No association was found between the proportion of energy delivered as propofol and outcomes.