bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cascade upconversion: a strategy enabling four-photon lithography in week light
Authors: Gan Zongsong , Li Shishuo , Li Kai , Yi Chenqi 
Published: October 2024

Abstract

Multiphoton lithography(MPL) offers the highest resolution available in submicron scale true 3D printing, but excessive femtosecond laser intensity prevents it from leading to higher photon counts. To circumvent away this effect, we present a cascade upconversion (CUC) strategy, which is a combination of two efficient two-photon upconversion processes to achieve four-photon photopolymerization. In order to demonstrate the advantages and feasibility of this approach, we combine excited state absorption upconversion using high concentration Ho3+/Yb3+ doped upconversion nanoparticles (UCNPs) with triplet-triplet annihilation (TTA) upconversion to fabricate 3D polymer structures by low-cost continuous wave 980 nm laser at 105 W/cm2. This method overcomes the diffusivity caused by isotropy of UCNPs luminescence, and achieves two orders of magnitude improvement in resolution while maintaining the advantages of using near infrared(NIR) light. This new strategy provides a general way for designing four-photon and even six-photon MPL in week light.
Microbial analyses of infectious keloids on the anterior chest— a case-control study
Authors: Chen Qian , Hou Shen , Wu Xiao-Yan , Bu Wen-Bo , Zhou Bing-Rong , Chen Xiao-Dong 
Published: October 2024

Abstract

Backgroud:
Some studies confirmed the presence of pathogen may cause infection by bacterial cultures on the surface of infectious keloids. But further exploration of comparison with non-infectious keloids and the bacterial flora of infectious foci was lacking.
Objective
To investigate the differential flora of purulent secretions on the surface of infectious keloids compared to the surface of non-infectious keloids. And to determine the microbial composition within the infectious foci.
Methods
This case-control study of 17 patients obtained swab specimens from the surface of keloids from two groups and the infectious foci from the infectious group. Bacterial composition was analyzed by 16S ribosomal RNA sequencing.
Results
There was no statistical difference in the general condition of patients from two groups. The number of the phylum Actinobacteriota, the order Propionibacteriales, Corynebacteriales, and the genus Taibaiella was more on the keloids surface in the infectious group. The three most represented genera in infective sites were the genus Staphylococcus, Peptoniphilus, and Cutibacterium. Microbial-associated gene pathways revealed a correlation with inflammation and tumor like growth of keloids.
Conclusion
There was a link between infectious keloids and microorganisms, providing direction for the prediction and treatment of keloid infections.
A Rare Case of Adult-Onset Vanishing White Matter Leukoencephalopathy With Movement Disorder
Authors: Douden Bashar Kamal Ali , Abufara Yazan Mohammad Abdullah , Aldrabeeh Mahmood Fayez Ali , Tell Naela Ramadan Mohammad , Abudaya Ismail 
Published: October 2024

Abstract

Background:
Vanishing white matter disease (VWMD) is a rare autosomal recessive leukoencephalopathy. It is typified by a gradual loss of white matter in the brain and spinal cord, which results in impairments in vision and hearing, cerebellar ataxia, muscular weakness, stiffness, seizures, and dysarthria cogitative decline. The majority of reports involve minors. Very few instances worldwide have been reported, with adult onset of vanishing white matter considered to account for 15% of cases. Clinical evaluation, MRI results, and confirmatory genetic testing are used to diagnose VWMD.
Case presentation:
A 39-year-old male from Hebron, Palestine, presented with a 7-month history of postural instability, imbalanced gait, and progressive deterioration of his lower extremities. Additionally, the patient suffered from ocular abnormalities and sphincteric issues. The patient's sibling showed comparable symptoms but was never diagnosed, as he passed away as a result of colon cancer, as per his family history. Reduced cognitive function, spastic quadriparesis, hyperreflexia, bradykinesia, and shuffling gait were found during a neurological examination. Normal results were obtained from routine laboratory tests, including cerebrospinal fluid (CSF), blood, and urine. Periventricular white matter hyperintensities, which are indicative of vanishing white matter leukoencephalopathy (VWML), were identified during an MRI. The diagnosis of adult-onset VWML with movement disability was substantiated by genetic testing, which named a homozygous pathogenic missense mutation, EIF2B3, and a deletion in PRKN/PARK2. The patient's motor symptoms were temporarily alleviated following the administration of Levodopa/Carbidopa. Nevertheless, the long-term consequences are uncertain due to the illness's ongoing progression and the absence of a cure at this time.
Conclusion:
This instance of vanishing white matter leukoencephalopathy (VWML) is particularly remarkable in adults because of its rarity and complexity. The diagnosis is further complicated by the coexistence of Parkinsonism and VWML. Although a cure is not currently known. Early discovery is crucial to effectively manage symptoms. This example underscores the importance of more VWML research, particularly in Palestine, where studies on neurological disorders are limited. These findings underscore the importance of enhancing the region's diagnostic and therapeutic capabilities.
Tooth loss related risk factors and oral microbiota changes in older adults: a cross-sectional study
Authors: Zhang Chenze , Jiang Jiapei , Huang Lirong , Cheng Xingqun , Wu Hongkun 
Published: October 2024

Abstract

Objectives
This study aimed to analyze the risk factors of tooth loss in the elderly, and to compare the structure and composition of the salivary microbiota between the low residual tooth group and the high residual tooth group, providing theoretical basis for the development of professional and effective preventive and management strategies to improve tooth loss in the elderly.
Materials and methods
A sexual cross-sectional study was conducted in 310 elderly people over 60 years old. Through clinical examination and questionnaire survey, the risk factors of tooth loss were collected. Monomial and polynomial logistic regression models were used to estimate the data. Non-irritant saliva samples were collected from people in low residual tooth group and high residual tooth group (n = 25/each group) for 16S rRNA sequencing analysis.
Results
310 elderly people were categorized into two groups based on their residual teeth count: low residual teeth group (n = 87) and high residual teeth group (n = 223). Tooth loss in the elderly is closely related to age, root caries, frequency of eating dessert and candies, toothpick use, self-rated oral health, and frailty according to the multifactorial analysis. In terms of microbial composition, the proportion of Porphyromonas, Treponema, Asticcacaulis, Filifactor and Prevotella in the low residual tooth group were significantly lower than that in the high residual tooth group.
Conclusion
Tooth loss is closely related to the aging, poor living habits, and poor oral and physical conditions of the elderly. The proportion of pathogenic bacteria in the elderly population with more tooth is higher.
Clinical relevance
This study provides some risk factors that can be directly assessed through clinical examination and chairside questionnaires, and provides guidance for subsequent laboratory testing of the microbiota composition of patients' saliva, which is conducive to timely knowledge popularization and long-term help for elderly patients.
YTHDF2 Promotes the Metastasis of Oral Squamous Cell Carcinoma through the JAK- STAT Pathway
Authors: chen zhezheng , Zhao Dan , yuan yamin , Zeng Lu , Luo Zhengzhou , Chen Junliang , Lan Xiaorong , He Yun , Liu Lin 
Published: October 2024

Abstract

Background: RNA-binding proteins act as crucial mediators between m6A modification and RNA function, playing a significant role in the recurrence and metastasis of oral squamous cell carcinoma. YTHDF2, the first identified RNA-binding protein, is associated with the prognosis of certain cancer patients. However, the role of YTHDF2 in oral squamous cell carcinoma and its underlying molecular mechanism have not been fully explored.
Methods: First, we analysed the expression levels of YTHDF2 and itscorrelation with clinical features via the use of public databases and oral squamous cell carcinoma patient samples. Subsequently, in vitro functional experiments were conducted to assess the effects of YTHDF2 on the proliferation, migration and invasion capabilities of oral squamous cell carcinoma cells. Additionally, RNA-seq analysis was utilized to investigate the signalling pathways modulated by YTHDF2, followed by experimental validation.
Results: Our findings revealed that YTHDF2 expression was significantly elevated in oral squamous cell carcinoma tissues and cells, with levels significantly correlated with the clinical stage, pathological grade, and survival time of patients. The knockdown of YTHDF2 resulted in decreased proliferation, migration and invasion abilities in oral squamous cell carcinoma cells. Furthermore, RNA sequencing data indicated that silencing YTHDF2 suppressed the JAK-STAT signalling pathway, and the use of STAT3 activators reversed this suppressive effect in oral squamous cell carcinoma cells.
Conclusion: Our study demonstrated that YTHDF2 promotes the proliferation, metastasis and invasion of oral squamous cell carcinoma by positively regulating the JAK-STAT signalling pathway, suggesting that YTHDF2 could serve as a potential prognostic marker for oral squamous cell carcinoma.
Demographic, clinical, treatment, outcome, and comorbidities of patients with relapsing polychondritis: Experience from Brazilian tertiary center, and literature review
Authors: Ueda Patricia Pilar Lury Ortale , Komati Luiz Antonio Leandrini , Shinjo Samuel Katsuyuki 
Published: October 2024

Abstract

Background Due to the rarity of relapsing polychondritis (RP), we described the demographic, clinical, treatment, outcomes, and comorbidities of patients with RP from our tertiary service. Additionally, a literature review was conducted.Methods A total of 47 Brazilian patients with RP between 2000 and 2024 were analyzed. All patient data were collected from pre-parametrized and pre-standardized electronic medical records. A literature review using PubMed with “relapsing polychondritis” as the search term included 25 articles after applying the strict exclusion criteria.Results A total of 47 patients were evaluated. The median age was 40 (34–51) years, with a female-to-male ratio of 1.4:1, and 89.4% were of white ethnicity. The median time from symptom onset to diagnosis was 39 months and the median follow-up duration was 7 years. Ear cartilage biopsy was performed in 12.8% of cases. The clinical manifestations included auricular chondritis, arthralgia, and ocular involvement. Approximately half of the patients had hypertension and dyslipidemia, one-third had diabetes mellitus, and one-fifth had hypothyroidism. Tracheostomy and cochlear implantation were required in 12.8% and 6.4% of the patients, respectively. Disease outcomes showed that 46.8% of patients were in remission, 29.8% had active disease, and 25.5% were controlled with immunosuppressive therapy. Mortality occurred in 4.3% of the cases. In the literature review, 25 studies were analyzed, most of which originated in Asia. Studies have reported the classical manifestations of RP, such as auricular chondritis, arthritis, and ocular involvement. The median age of the patients with RP was similar across studies, averaging 46.4 years, with a predominance of female patients. A comparison with the literature showed consistency in clinical manifestations, particularly auricular chondritis and septum nasal chondritis, although few studies have explored comorbidities, disease evolution, and outcomes.Conclusions The reviewed articles described classical clinical manifestations, but few articles reported data on other manifestations and comorbidities that can occur in RP. Our study provided new insights by mapping symptom evaluations, thereby enhancing the understanding of disease evolution. Understanding and characterizing RP will allow for better assistance in its diagnosis and follow-up.
Review of Intrauterine Insemination in Polycystic Ovarian Syndromes - Cases Review and Current Approach
Authors: Suryawan Alfonsus Zeus , Susilo Artha Falentin Putri , Djuwantono Fridya Wulandari 
Published: October 2024

Abstract

Intrauterine insemination (IUI) is procedure which we introduce sperm into the fundal cavity. It’s help a lot of infertile couple to reach clinical pregnancy and currently mainly first choice of unexplained fertility. Usage in other cause of infertility was also consider with individualized approach. However IUI is not without flaw, it’s success rate rely heavily on sperm count and motility. It’s usage in polycystic ovary syndrome (PCOS) still in debates, due to high insulin resistance condition in PCOS hinders implantation and increase risk of fetal loss associated with endometrial dysfunction. This serial case report shows want to review 4 cases of IUI with PCOS on Aster Fertility Clinic from September 2023 – July 2024 and current approach of this condition which relevant to nowadays practice and knowledge.
The effect of mastoid vibration on joint angles of lower extremities during walking: implication for sensorimotor training for future spaceflight
Authors: Xie Haoyu , Wang Zhuo , Wang Chuhuai , Chien Jung Hung 
Published: October 2024

Abstract

A spaceflight in microgravity unloads the peripheral vestibular organs and disturbs the vestibular system of astronauts. This disruption of the vestibular system forces astronauts to use different strategies to walk, such as flexing more on their knee joints to increase force to propel their bodies forward on the Moon. It should be noted that this vestibular disruption observed in microgravity cannot easily be replicated on Earth. However, mastoid vibration (MV) has been shown to be an effective alternative method for disrupting vestibular function. The application of MV during walking has been demonstrated to alter gait characteristics regardless of the different terrains. Could these changes in gait characteristics induced by MV also affect joint angle patterns in a similar manner? Therefore, the purpose of this study was to fill this knowledge gap. This study involved fifteen young adults and ten older adults. Two motion capture cylinders were used to record the trajectory of 10 body land markers. Two electromechanical vibrotactile transducers were placed on bilateral mastoid processes to generate the MV at 100Hz. A reduced-light intensity goggle was used to reduce the visual capability during walking. A total of six 2-minute walking trials were randomly assigned to each participant (3 MV: bilateral/unilateral/no MV x 2 vision levels: full/reduced vision). Dependent variables included peak values, range of motion (ROM), and respective hip, knee, and ankle joint variabilities. A significant interaction between the effects of aging, vision level, and different types of MV was observed in the peak hip flexion angle (p = 0.001). Post hoc comparisons indicated that 1) applying bilateral MV significantly decreased peak hip flexion of healthy young but increased peak hip flexion of healthy young in reduced vision condition, and 2) applying bilateral MV induced a greater effect on decreasing hip flexion than applying unilateral MV in all participants regardless of whether vision was full or reduced. The findings of this study revealed the important role of hip joints in balance control during gait under vestibular and/or visual perturbation. Also, MV could be used to simulate a vestibular-disrupted environment for astronauts for future sensorimotor training.
Bionic fractionalization in the trimer model of twisted bilayer graphene
Authors: Kim Eun-Ah , Zhang Kevin , Mao Dan , Moessner Roderich 
Published: October 2024

Abstract

Motivated by the rapid experimental progress in twisted van der Waals materials, we study the triangular trimer model as a representative framework for extended Wannier orbitals in twisted bilayer graphene at 1/3-filling. This deceptively simple model exhibits a rich suite of complex phases, including unusual excitations exhibiting the physics of fractionalization and fractons. For our investigations, we carry out extensive Monte Carlo simulations using an efficient cluster algorithm. The so-obtained finite-temperature phase diagram reveals a novel polar fluid and an ordered brick-wall phase characterized by fractionally charged e/3 excitations with subdimensional lineonic dynamics. Notably, we identify a critical trimer liquid phase for the particularly simple model of hard trimers. For this, we derive a new field theory which takes the form of a U(1)×U(1) gauge theory. Its e/3 monomers are fractionalized bionic excitations: they carry a pair of emergent gauge charges, as evidenced by algebraic correlations with two distinct exponents. These field theoretical predictions offer theoretical grounds for numerical observations of critical exponents. Our study highlights the triangular trimer model as a new key platform for investigating fractionalization and fractons, where trimer liquid bionic monomers can transform into lineons or fractons in proximate phases, and calls for experimental investigations of this physics in twisted van der Waals materials and a broader class of systems with intermediate-range interactions.
Awareness towards obstetric fistula and associated factors among reproductive age group women in Bibugn district, East Gojjam, Amhara, Northwest Ethiopia; Cross sectional study
Authors: Kassahun Bezawit , Degu Genet , Talie Asmare , Chanyalew Lemlem 
Published: October 2024

Abstract

Background
Obstetric fistula is a hole which develops between female private organs and leaves a woman incontinent of urine or faeces or both. It is a devastating public health problem usually caused by obstructed labour. Its occurrence is correlated with lack of awareness. The key to eliminate obstetric fistula is raising awareness of it and the factors that contribute to it.
Objective
To assess awareness towards obstetric fistula and associated factors among reproductive age women in Bibugn district, East Gojjam zone, Amhara, Ethiopia, 2022.
Methods
A community based cross-sectional study was employed to collect data from 612 reproductive age women from May20-June20/2022. A systematic sampling technique was used to select study participants. Data were collected using a pretested structured face to face interviewer- administered questionnaire. Bivariable and multivariable analysis were used to identify factors associated with the dependant variable.
Result
From total sampled participants, of them 608(99.3%) were interviewed, from those 37.8%[95%CI (34, 42%)]study participants had good awareness towards obstetrics fistula. Primary andsecondaryeducation(AOR = 4.91,CI = 3.10,7.99), and (AOR = 6.37, CI = 3.70,10.91) respectively, being urban resident (AOR = 1.71,CI = 1.09,2.67), contraceptive use (AOR = 1.70,CI = 1.04, 2.79), and distance < 30minute on foot from health facility(AOR = 0.42,CI = 0.34,0.79) weresignificantly associated with awareness towards obstetric fistula.
Conclusion and Recommendation
This study reported that awareness of obstetrics fistula was low compared to previous studies in Ethiopia. Urban residency, education (primary and above), having a history of using contraceptives and proximity to a health facility were significantly associated with awareness towards obstetric fistula. Measures to improve educational level and contraceptive utilization of reproductive age women should be considered.
High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma
Authors: Bourdier Solenne , Fisch Anne-Sophie , Alp Keziban Merve , Das Ridhima , Mertins Philipp , Tinhofer Ingeborg 
Published: October 2024

Abstract

Human papilloma virus-negative head and neck squamous cell carcinoma (HNSCC) frequently harbors 11q13 amplifications. Among the oncogenes at this locus, CCND1 and ANO1 are linked to poor prognosis; however, their individual roles in treatment resistance remain unclear. The impact of Cyclin D1 and Ano1 overexpression on survival was analyzed using the TCGA HNSCC dataset and a Charité cohort treated with cisplatin (CDDP)-based radiochemotherapy. High Ano1 expression was primarily associated with poor overall survival in both datasets. The effects of CCND1 and ANO1 knockdown (KD) on radio- and drug sensitivity, along with changes in global protein expression, cell viability, growth, and DNA repair, were studied in an 11q13-amplified HNSCC cell line model of primary cisplatin resistance. Unique pathway alterations– VEGF in CCND1 KD and the Rho GTPase cycle in ANO1 KD– were observed, along with shared changes like DNA damage and cell cycle dysregulation. Silencing Cyclin D1 or Ano1 increased CDDP sensitivity, while only Ano1 silencing increased radiosensitivity. Copanlisib and afatinib were identified as promising candidates for combination therapy of 11q13-amplified HNSCC tumors. We demonstrated a predominant role for Ano1 in treatment resistance in Cyclin D1highAno1high HNSCC tumors and identified novel potential treatment combinations for this high-risk patient group.
Intraocular lens position stability during phacovitrectomy with air or gas tamponade
Authors: Yoshikawa Yuji , Matsushima Takashi , Takano Shunichiro , Makita Jun , Shinoda Kei 
Published: October 2024

Abstract

To evaluate the stability of different intraocular lens (IOL) after phacovitrectomy with air or gas tamponade.Twenty-eight patients who underwent phacovitrectomy with air or sulfur hexafluoride (SF6) gas tamponade and who underwent postoperative anterior segment optical coherence tomography assessments using CASIA2 (TOMEY, Inc., Japan), were included in the study.Patients who received any of DIB00V (Johnson & Johnson Surgical Vision, Inc., USA), XY-1 (HOYA Surgical Optics Co., Japan), or NX70 (Santen Pharmaceutical Co.) implantation were analyzed. The the anterior chamber depth (ACD) and IOL position values at each measurement time point (100%, 50%, 0%) and between IOLs were compared. The IOL displacement force (mN) was measured with verification experiment and recorded at each 0.1 mm anterior movement until the anterior displacement reached 0.5 mm. The overall age was 66 (59, 73) (median [quartiles])years. The ACD values increased progressively as the postoperative gas decreased for all IOLs. Compared to the preoperative ACD, the postoperative ACD was significantly greater at 50% and 0% gas for all IOLs (P < 0.01). Additionally, DIB00V had significantly larger ACD values compared to XY-1 at 100% gas or air (P=0.023) and NX70s at 0% gas or air (P=0.008). The IOL position shifted significantly posteriorly for both DIB00V and XY-1 as the gas or air dissappeared (P<0.05). However, for NX70s, the position significantly increased from 100% to 50% (P<0.01), with no significant difference from 50% to 0% (P=0.444). IOL-by-IOLs comparisons at each time point showed that DIB00V was significantly more posterior than XY-1 at 100% (P=0.046). In the verification experiment, DIB00V showed a greater IOL displacement force against a push from the retinal surface compared to XY-1 and NX70s.DIB00V exhibited excellent Z-axis stability in phacovitrectomy with air or gas tamponade.