bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering Regulatory Role of Melatonin in Foliar Nastic Movements of Portulaca oleracea: Visual Insights from Histology and Anatomy
Authors: Bargunam Soundaryaa , Roy Riyan , Amisha S. H. , Shetty Devika , Babu Vidhu Sankar 
Published: October 2024

AbstractPlant nastic movements exhibit unique behavioural patterns that synchronize with external cues. Given that the foliar nastic motions of Portulaca species are solely circadian, it is intriguing to investigate whether and how melatonin governs these movements. Analysis of histological traits concurrent with anatomical traits such as stomatal behaviour provides visual data on the plant species' gnosophysiology, offering clues and validation of the influence of multiple external stimuli on hydraulic forces that in turn alter turgor pressure. The current study aims to elucidate how exogenous melatonin modulates foliar nastic movements in Portulaca oleracea. Our findings indicate that melatonin functions as an intracellular hydraulic flux controller, influencing idioblast and crystal densities, as well as stomatal behaviour. Timepoint studies at specific Zeitgebers reveal that abiotic variables such as light and temperature can influence the endogenous melatonin concentration of P. oleracea. Therefore, this hormone potentially serves as an additional internal regulator of turgor pressure, influenced by both light and temperature. Thus, melatonin plays a crucial role in shaping the characteristic foliar nastic movements observed in P. oleracea, inherently tied to the circadian rhythm. Further investigation into idioblast and crystal torques, along with their angular momentum, is warranted to calculate the hydraulic forces at work in the leaf lamina. Thus the study underscores the multifaceted role of melatonin in navigating nastic movement processes through turgor pressure alterations brought about by intracellular depositions. The study in future could unravel melatonin's pleiotropic actions and the underlying mechanisms of foliar idiosyncratic nastic motions in other plant species as well. Moreover, both histology and anatomy play vital roles in the present study as it has provided visual evidence of the underlying mechanisms of foliar nastic movements in P. oleracea.
Graphical Abstract
TRPC3-mediated NFATc1 calcium signaling promotes triple negative breast cancer migration through regulating glypican-6 and focal adhesion
Authors: Wang Yan , Zhuang Xiaosheng , Qi Yanxiang , Yiu Lung , Li Zhenping , Chan Yuk Wah , Liu Xianji , Tsang Suk Ying 
Published: October 2024

Abstract
Canonical transient receptor potential isoform 3 (TRPC3), a calcium-permeable non-selective cation channel, has been reported to be upregulated in breast cancers and a modulator of cell migration. Calcium-sensitive transcription factor NFATc1, which is important for cell migration, was shown to be frequently activated in triple negative breast cancer (TNBC) biopsy tissues. However, whether TRPC3-mediated calcium influx would activate NFATc1 and affect the migration of TNBC cells, and, if yes, the underlying mechanisms involved, remain to be investigated. By immunostaining followed by confocal microscopy, TNBC lines MDA-MB-231 and BT-549 were both found to express TRPC3 on their plasma membrane while ER+ line MCF-7 and HER2+ line SK-BR3 do not. Blockade of TRPC3 by pharmacological inhibitor Pyr3 or stable knockdown of TRPC3 by lentiviral vector both inhibited cell migration as measured by wound healing assay. Importantly, blocking TRPC3 by Pyr3 or knockdown of TRPC3 both caused the translocation of NFATc1 from the nucleus to the cytosol as revealed by confocal microscopy. Interestingly, NFATc1 was found to bind to the promoter of glypican 6 (GPC6) as determined by chromatin immunoprecipitation assay. Consistently, knockdown of TRPC3 decreased the expression of GPC6 as revealed by western blotting. Moreover, long-term knockdown of GPC6 by lentiviral vector also consistently decreased the migration of TNBC cells. Intriguingly, GPC6 proteins physically interact with vinculin in MDA-MB-231 as determined by co-immunoprecipitation. Blockade of TRPC3, knockdown of TRPC3 or knockdown of GPC6 all induced larger, stabilized actin-bound peripheral focal adhesion (FA) formations in TNBC cells as determined by co-staining of actin and vinculin followed by confocal microscopy. These large, stabilized actin-bound peripheral FAs indicated a defective FA turnover, and were reported to be responsible for impairing directed cell migration. Our results suggest that, in TNBC cells, calcium influx through TRPC3 channel positively regulates NFATc1 nuclear translocation and GPC6 expression, which maintains the dynamics of FA turnover and optimal cell migration. Our study reveals a novel TRPC3-NFATc1-GPC6-vinculin signaling cascade in maintaining the migration of TNBC cells.
Fluid and burns in children: What we know and what we do not know—a retrospective analysis of the German Burn Registry from 2015 to 2022
Authors: Vasileiadis Vasileios , Najem Safiullah , Reinshagen Konrad ,   the German Burn Registry, Aigner Annette , Koenigs Ingo 
Published: October 2024

Abstract Fluid resuscitation is of great importance in the management of major burns. Various formulae have been described for calculating fluid management, especially in severely burned patients. Although the Parkland formula is widely used, its efficacy and clinical value are discussed controversially. We investigated the impact of deviation from calculated fluid volume by Parkland formula and the maintenance i.v. fluid (Parkland*) on the outcome of burned pediatric patients. Patients aged < 16 years with thermal injuries included in the German Burn Registry between January 2016 and December 2022 with a total body surface area ≥ 15% were analyzed. Using mixed-effect negative binomial regression, the association between a deviation from Parkland* in the administered fluid volume and the primary outcome length of hospital stay was estimated—additionally adjusted for known risk factors. As a secondary outcome, we use in-hospital mortality, evaluated descriptively. In 86.5% of patients, the administered fluid volume was lower than Parkland*, with pronounced deviation in the seven patients who died in the hospital. Descriptively and based on mixed-effect negative binomial regression models, we found that a positive deviation from Parkland* increases the number of hospital days, whereas a negative deviation may decrease them. Conclusion: Very little is known about the role of administered resuscitation volumes for the outcome of pediatric severely burned patients. This study observed a tendency to a restricted resuscitation and its potential benefits in terms of length of hospital stay.

What is Known:• Fluid resuscitation is an important aspect of therapy in the acute phase of children with extensive burns.• The Parkland formula is routinely used formula for determining fluid requirements.

What is New:• 86.5 % received less volume than determined by Parkland and a tendency to restricted resuscitation and its potential benefits in terms of length of stay was observed.• There is still considerable lack of clarity, and a strictly individualized protocol with the support of formulas is of crucial importance.
Increased inter-atrial and intra-atrial conduction times in pediatric patients with non-alcoholic fatty liver disease
Authors: El Amrousy Doaa , EL Ashry Heba , Maher Sara , Elsayed Yousef , Elkashlan Karim , Abdelhai Dina , Mawlana Wegdan , Hasan Samir 
Published: October 2024

AbstractThe global incidence of pediatric non-alcoholic fatty liver disease (NAFLD) is rising, and it is linked to various potentially dangerous complications such as cardiovascular complications, particularly atrial fibrillation (AF). Atrial electromechanical conduction delay (EMD) has been reported as an early predictor for AF development. This study aimed to explore the link between NAFLD and the increased risk of AF development. This cross-sectional study was performed on 100 newly diagnosed NAFLD children (aged 14–18 years) as the patient group and 100 healthy individuals as a control group. Transthoracic echocardiography and simultaneous electrocardiography (ECG) recording were employed to estimate atrial electromechanical properties. EMD values were calculated for the inter-atrial, left intra-atrial, and right intra-atrial. Our results showed that pediatric patients with NAFLD exhibited significantly prolonged EMD values in the left and right intra-atrial as well as in inter-atrial regions compared to the control group (P = 0.03, P < 0.001, P < 0.01, respectively). Conclusion: Children with NAFLD show atrial electromechanical alterations that may presage AF in adulthood.

What is known:• The global incidence of pediatric non-alcoholic fatty liver disease (NAFLD) is rising, and it is linked to various potentially dangerous complications such as cardiovascular complications, particularly atrial fibrillation (AF).• Atrial electromechanical conduction delay (EMD) has been reported as an early predictor for AF development.What is new:• Children with NAFLD show atrial electromechanical alterations that may presage the appearance of AF in adulthood.
• These children require multidisciplinary medical care to control liver disease and cardiovascular complications.
From ABC to KPZ
Authors: Cannizzaro G. , Gonçalves P. , Misturini R. , Occelli A. 
Published: October 2024

Abstract
We study the equilibrium fluctuations of an interacting particle system evolving on the discrete ring with

$$N\in {\mathbb {N}}$$

N
∈
N

points, denoted by

$${\mathbb {T}}_N$$

T
N

, and with three species of particles that we name A, B and C, but such that at each site there is only one particle. We prove that proper choices of density fluctuation fields (that match those from nonlinear fluctuating hydrodynamics theory) associated to the (two) conserved quantities converge, in the limit

$$N\rightarrow \infty $$

N
→
∞

, to a system of stochastic partial differential equations, that can either be the Ornstein–Uhlenbeck equation or the Stochastic Burgers equation. To understand the cross interaction between the two conserved quantities, we derive a general version of the Riemann–Lebesgue lemma which is of independent interest.
Digitalisierung in Lebenswissenschaften und Medizin – das Dual-Use-Problem
Authors: Heinrichs Jan-Hendrik , Ergin Aslan Serap 
Published: October 2024

ZusammenfassungDual Use bezeichnet zunächst die Verwendbarkeit eines eigentlich für andere Zwecke intendierten Forschungsergebnisses oder -verfahrens für Zwecke, die die innere oder äußere Sicherheit einer Gesellschaft betreffen. Darunter fällt Forschung, die für militärische, geheimdienstliche, terroristische oder kriminelle Zwecke verwendet werden kann. Dual Use stellt seit über einem Jahrzehnt ein sich beständig verschärfendes Problem für viele Bereiche der Lebenswissenschaften und der Medizin dar, weil viele von deren Ergebnissen geeignet sind aufzuzeigen, wie Menschen, aber auch (Nutz‑)Tiere und Pflanzen manipuliert und attackiert werden können und welche Werkzeuge dafür besonders geeignet sind.Die Digitalisierung in den Lebenswissenschaften hat im Hinblick auf das Dual-Use-Problem zwei voneinander unterscheidbare Einflüsse, nämlich einen verschärfenden und einen erweiternden. Dadurch, dass die Digitalisierung schnellere und umfangreichere Forschungs- und Entwicklungsprozesse ermöglicht, verschärft sie das bestehende Dual-Use-Problem, weil zugleich das Tempo steigt, in dem die Ergebnisse dieser Forschung für sicherheitsrelevante Zwecke nutzbar gemacht werden können. Zudem erweitert die Digitalisierung der Lebenswissenschaften das Dual-Use-Problem, denn einige der digitalen Werkzeuge, die in den Lebenswissenschaften entwickelt und gebraucht werden, können selbst militärische oder sicherheitsrelevante Verwendung finden.Deshalb bedarf es einer breit angelegten Governance inklusive einer breiten Beteiligung der Stakeholder im Forschungsprozess und eines Informationsangebots zu Dual Use in der Ausbildung in guter wissenschaftlicher Praxis über Institutionen, Karrierestufen und Disziplinen hinweg.
Implementing the Resource Nexus approach: the contribution of internationalising German businesses
Authors: Schneider Christian P. 
Published: October 2024

Abstract
The Resource Nexus is an approach to sustainably manage environmental resources by considering the interlinkages, trade-offs and synergies between them. While a lot is known about this Resource Nexus framework and the impact this has on sustainable development and politics, far less is known about the technological, economic, and political situation required for the implementation of this approach. This paper considers the role of international business cooperation between German and Sub-Saharan African firms in facilitating this implementation. It explores the major barriers faced by German businesses looking to work in Sub-Saharan Africa and analyses the work of the German Außenhandelskammern (German Chambers of Commerce Abroad) as a replacement for the networks that are commonly seen as vital to business internationalisation. The findings have far-reaching implications for German and Sub-Saharan African politics, for business practices, and for the work of intermediary organisations looking to facilitate the internationalisation, as well as for those actors looking to implement a Resource Nexus approach across the world.
Characteristics and outcomes of inpatients aged 85 and older with thoracolumbar vertebral fractures: impact on hospital stay and mortality
Authors: Gräschke Erik , Jarvers Jan-Sven , Heyde Christoph-Eckhard , Spiegl Ulrich Albert Joseph 
Published: October 2024

Abstract
Background
There is a gap in evidence about medical outcomes in oldest-old patients (aged 85 and older) with vertebral fractures (VFs). The aim of this study was to evaluate the impact of patient and fracture characteristics on “short-term” hospital outcomes.

Methods
All patients aged ≥ 85 presenting an acute or subsequent VF at our single level I spine center between 2019 and 2021 requiring hospital treatment were included. The data collection was conducted retrospectively. The primary parameters of interest were length of stay (LOS) and in-hospital mortality. Further outcome parameters were the occurrence of general (non-operative) complications and Intensive Care Unit (ICU) admission. For statistical analysis, linear and binary logistic regression modeling were performed.

Results
A total of 153 patients with an average age of 88.5 (range 85 to 99) met the inclusion criteria. Our patients were mostly female (68.6%) and moderately comorbid according to a Charlson Comorbidity Index (CCI) of 2.9. 58.8% had diagnosed osteoporosis. Fracture morphologies represented as “Osteoporotic Fracture” (OF) classification types were of central importance for undergoing operative treatment (OP) (p < 0.001), necessity for intensive care (p = 0.023), LOS (p = 0.014), and mortality (p = 0.018). 38.6% had OP. We recorded a complication rate of 59.5%, which highly influenced (p < 0.001) both primary outcome parameters. Overall, patients stayed 14.6 days with a mortality of 11.1%.

Conclusion
VFs are a severe event in oldest-old patients with a crucial risk of poor medical outcomes during hospitalization. The fracture morphologies are of central importance. However, little is known about the hospital stay of oldest-old inpatients with VFs. Considering an aging population, further investigations would be recommended.
An optimal ansatz space for moving least squares approximation on spheres
Authors: Hielscher Ralf , Pöschl Tim 
Published: October 2024

AbstractWe revisit the moving least squares (MLS) approximation scheme on the sphere $$\mathbb S^{d-1} \subset {\mathbb R}^d$$

S

d
-
1

⊂

R

d

, where $$d>1$$

d
>
1

. It is well known that using the spherical harmonics up to degree $$L \in {\mathbb N}$$

L
∈
N

as ansatz space yields for functions in $$\mathcal {C}^{L+1}(\mathbb S^{d-1})$$

C

L
+
1

(

S

d
-
1

)

the approximation order $$\mathcal {O}\left( h^{L+1} \right) $$

O

h

L
+
1

, where h denotes the fill distance of the sampling nodes. In this paper, we show that the dimension of the ansatz space can be almost halved, by including only spherical harmonics of even or odd degrees up to L, while preserving the same order of approximation. Numerical experiments indicate that using the reduced ansatz space is essential to ensure the numerical stability of the MLS approximation scheme as $$h \rightarrow 0$$

h
→
0

. Finally, we compare our approach with an MLS approximation scheme that uses polynomials on the tangent space of the sphere as ansatz space.
Prioritization of important plant areas for conservation of frailejones (Espeletiinae, Asteraceae) in the Northern Andes
Authors: Morales-Rozo Clara , Acuña-Rodríguez Ian S. , Benavides Juan C. , Rincón-Parra Víctor J. , Diazgranados Mauricio 
Published: October 2024

AbstractThe tropical Andes region harbors areas that are highly significant for biodiversity at both global and local scales. However, despite the severe conservation threats that this region has to confront, conservation initiatives are limited, by funding and because of the need for more information to decide which areas are priorities for conservation. Identifying Important Plant Areas (IPAs) offers a valuable methodology for establishing conservation priorities, a particularly complex task in a mega-biodiverse region such as the northern Andean tropics. Due to its iconic recognition and conservation value, this study focused on the Espeletiinae subtribe (Asteraceae) as a model group; hence, the distribution data for 138 species was compiled from 5560 georeferenced records. Using the IPA methodology, we divided the study area, this is, the distribution area of the subtribe in the Andean tropics, into 220 Units of Analysis (UA) represented by 10 × 10 km plots. Refined species’ distribution areas, incorporating richness, threatened species, and ecosystem-based richness distributions, were analyzed using newly generated maps to evaluate the conservation value of each UA. Our analysis identified 176 UAs with some level of relevance using sub-criterion cA1 (with 59 species) and 51 UAs using sub-criterion cB (with 76 species). Integrating both criteria, we classified 11 UAs as high-priority, 62 as medium-priority, and 147 as low-priority, highlighting the IPAs that require focused conservation efforts. Two identified high-priority IPAs are located in Venezuela and nine in Colombia, predominantly associated with the Eastern Cordillera. These areas concentrate between 7 and 12 species. They are mainly linked to the páramo complexes of Colombia and the Sierra Nevada in Venezuela, primarily within Cool Temperate Moist Grassland ecosystems on mountains. Our results provide a spatial planning procedure and analytical tool for decision-makers to guide conservation management and actions across northern Andes.
Exploring the role of project status information in effective code smell detection
Authors: Alkharabsheh Khalid , Alawadi Sadi , Crespo Yania , Taboada José A. 
Published: October 2024

AbstractRepairing code smells detected in the code or design of the system is one of the activities contributing to increasing the software quality. In this study, we investigate the impact of non-numerical information of software, such as project status information combined with machine learning techniques, on improving code smell detection. For this purpose, we constructed a dataset consisting of 22 systems with various project statuses, 12,040 classes, and 18 features that included 1935 large classes. A set of experiments was conducted with ten different machine learning techniques by dividing the dataset into training, validation, and testing sets to detect the large class code smell. Feature selection and data balancing techniques have been applied. The classifier’s performance was evaluated using six indicators: precision, recall, F-measure, MCC, ROC area, and Kappa tests. The preliminary experimental results reveal that feature selection and data balancing have poor influence on the accuracy of machine learning classifiers. Moreover, they vary their behavior when utilized in sets with different values for the selected project status information of their classes. The average value of classifiers performance when fed with status information is better than without. The Random Forest achieved the best behavior according to all performance indicators (100%) with status information, while AdaBoostM1 and SMO achieved the worst in most of them (> 86%). According to the findings of this study, providing machine learning techniques with project status information about the classes to be analyzed can improve the results of large class detection.
Nutrient circularity of an anaerobic digestion project in a typical intensive rice cultivation village in the Vietnamese Mekong delta region: a regional material flow analysis and discussion of modeling practices
Authors: Fritze Albrecht , Rotter Vera Susanne 
Published: October 2024

AbstractRice is the third largest agricultural commodity in the world. Intensive rice production is associated with high fertilizer application, and vast quantities of rice straw are often disposed of by open burning. As emissions of N and P from fertilizer and their impacts are highly spatially disparate, it is crucial to locally assess potential solutions for nutrient circularity. A model framework was established to evaluate the potential impact of local N, P and C cycles along with suitable circularity indicators. This framework was applied for a case study in the typical intensive rice cultivation village Hậu Mỹ Bắc B in the Mekong Delta using STAN modeling software. A novel technique, based on a patented temperature-phased anaerobic digestion (TPAD) loop-reactor design was investigated as a measure to reduce rice straw open burning, generate bioenergy and recover digestate for nutrient recirculation. The case study showed that rice straw TPAD could reduce open burning by 60%, while the N recovery rate increased to 21% when 200 ± 34 Mg/a of inorganic fertilizer was replaced with digestate. For C and P, the recovery rates increased to 14% and 11%, respectively. Based on the results, we concluded that TPAD has a strong impact on local nutrient circularity in terms of recirculation and sufficiency. Furthermore, the developed framework is suitable to use for master planning of nutrient circularity and for technological impact assessments at local scale. However, versatile, dynamic, and comparable monitoring frameworks with harmonized indicators to identify options for nutrient circularity are required for the detailed modeling of regional nutrient management.