bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulating IL-17F, but not IL-17A, is elevated in severe COVID-19 and leads to an ERK1/2 and p38 MAPK-dependent increase in ICAM-1 cell surface expression and neutrophil adhesion on endothelial cells
Authors: Bédard-Matteau Jérôme , Soulé Antoine , Liu Katelyn Yixiu , Fourcade Lyvia , Fraser Douglas D. , Emad Amin , Rousseau Simon 
Published: October 2024

BackgroundSevere COVID-19 is associated with neutrophilic inflammation and immunothrombosis. Several members of the IL-17 cytokine family have been associated with neutrophilic inflammation and activation of the endothelium. Therefore, we investigated whether these cytokines were associated with COVID-19.MethodsWe investigated the association between COVID-19 and circulating plasma levels of IL-17 cytokine family members in participants to the Biobanque québécoise de la COVID-19 (BQC19), a prospective observational cohort and an independent cohort from Western University (London, Ontario). We measured the in vitro impact of IL-17F on intercellular adhesion molecule 1 (ICAM-1) cell surface expression and neutrophil adhesion on endothelial cells in culture. The contribution of two Mitogen Activated Protein Kinase (MAPK) pathways was determined using small molecule inhibitors PD184352 (a MKK1/MKK2 inhibitor) and BIRB0796 (a p38 MAPK inhibitor).ResultsWe found increased IL-17D and IL-17F plasma levels when comparing SARS-CoV-2-positive vs negative hospitalized participants. Moreover, increased plasma levels of IL-17D, IL-17E and IL-17F were noted when comparing severe versus mild COVID-19. IL-17F, but not IL-17A, was significantly elevated in people with COVID-19 compared to healthy controls and with more severe disease. In vitro work on endothelial cells treated with IL-17F for 24h showed an increase cell surface expression of ICAM-1 accompanied by neutrophil adhesion. The introduction of two MAPK inhibitors significantly reduced the binding of neutrophils while also reducing ICAM-1 expression at the surface level of endothelial cells, but not its intracellular expression.DiscussionOverall, these results have identified an association between two cytokines of the IL-17 family (IL-17D and IL-17F) with COVID-19 and disease severity. Considering that IL-17F stimulation promotes neutrophil adhesion to the endothelium in a MAPK-dependent manner, it is attractive to speculate that this pathway may contribute to pathogenic immunothrombosis in concert with other molecular effectors.
Purtscher-like retinopathy and paracentral acute middle maculopathy associated with improper antihypertensive drug use: a case report
Authors: Meng Yang , Sawut Abdulla , Tian Miao , Li Ying , Cai Liwei , Xiao Di , Yi Zuohuizi , Chen Changzheng 
Published: October 2024

BackgroundPurtscher-like retinopathy (PLR) is a rare retinal microangiopathy with unclear pathogenesis. Paracentral acute middle maculopathy (PAMM) is an optical coherence tomography (OCT) sign proposed in recent years, which is characterized by infarction of the middle layer of the retina. This article reported a rare case of PRL and PAMM probably related to improper antihypertensive drug use in a middle-aged male.Case presentationA 49-year-old man presented with a complaint of sudden-onset vision loss and paracentral scotomas in the right eye for approximately 1 week. At presentation, the best-corrected visual acuity (BCVA) was 20/63 OD and 20/20 OS. Fundus examination showed multiple cotton-wool spots and Purtscher flecken in the posterior segment of the right eye. OCT revealed hyper-reflectivities in the inner nuclear layer (INL), consistent with PAMM. En face OCT showed PAMM’s characteristic “fern-like” perivenular changes. Fluorescein angiography demonstrated prolonged arm-to-retina time, delayed artery and venous filling, and hypofluorescence corresponding to cotton-wool spots. Examinations of the left eye were unremarkable. Many imaging and laboratory tests were performed to detect the possible cause of PLR and PAMM, but no possible explanation was found except improper antihypertensive drug use. The patient was recommended to stop his antihypertensive medication, and prescribed other systemic medicines, including oral prednisolone (40 mg q.d. with gradual tapering), oral cobalamin (0.5 mg t.i.d.), and subcutaneous injections of compound anisodine (2.0 mL q.d.) beside the superficial temporal artery. Two weeks after onset, his BCVA improved to 20/25 in the right eye. During follow-ups, his BCVA recovered to 20/20, accompanied by the regression of fundus lesions. The patient reported no treatment-related adverse effects.ConclusionThis is the first reported case of PLR and PAMM following improper antihypertensive drug use. Our report expands our understanding of the etiology and pathophysiology of PLR and PAMM. We also stress the importance of proper application of medications in clinical practice.
Bibliometric and visual analysis of diabetic keratopathy research: trends, collaborations, and future directions
Authors: Zhenyu Wang , Jing Gao , Tianhong Wu 
Published: October 2024

PurposeDiabetic keratopathy has gained increasing attention due to advancements in diagnostic and therapeutic techniques a. This article presents a visual and bibliometric analysis to illustrate the knowledge network, research hotspots, trends, and potential future directions in this field.MethodsWe retrieved articles published since 2000 from the Web of Science and analyzed the authors, institutions, countries, keywords, citations, and co-citations of these articles with VOSviewer and CiteSpace.ResultsA total of 706 highly relevant articles were identified, with the United States, China and England as major contributors; the University of Manchester, Queensland University of Technology and Weill Cornell Medical−Qatar as key institutions; and Malik Rayaz, Efron Nathan and Ferdousi Maryam as prominent authors. High-citation articles have focused mainly on corneal confocal microscopy and diabetic peripheral neuropathy. Keywords form two clusters: one around complications, diabetes and cornea sensitivity, and another around corneal confocal microscopy and peripheral neuropathy.ConclusionThe identification of diabetic peripheral neuropathy via corneal confocal microscopy has been a major focus of research in this field, but the mechanisms underlying diabetic corneal neuropathy still require further investigation and breakthroughs.
Oxidative stress and inflammation mediate the association between elevated oxidative balance scores and improved sleep quality: evidence from NHANES
Authors: Zhang Qu , Yi Jing , Wu Yemei 
Published: October 2024

BackgroundThe association between oxidative stress, as measured by the Oxidative Balance Score (OBS), and sleep quality remains unclear. The primary objective of this investigation was to clarify this relationship and to explore the potential involvement of oxidative stress and inflammation.MethodsData from 15,198 participants in the National Health and Nutrition Examination Survey 2007–2014 were analyzed. Sleep quality indicators, including sleep disorder, trouble, and duration, were assessed. The OBS, comprising information on 16 dietary nutrients and 4 lifestyle factors, was then calculated. Multivariable logistic and linear regression models were employed to investigate the correlation between OBS and sleep quality. Additionally, mediation analyses were conducted to evaluate the potential effects of oxidative stress and inflammation.ResultsWe demonstrated a correlation between an elevated OBS and reduced sleep disorders (OR, 0.72; 95% CI, 0.58–0.91; p = 0.0055), reduced sleep trouble (OR, 0.81; 95% CI, 0.69–0.96; p = 0.0174), and prolonged sleep duration (β 0.009; 95% CI, 0.0002–0.0160; p = 0.015) when comparing the highest and lowest tertiles. Dietary factors exhibited autonomous correlations with sleep duration, whereas lifestyle factors displayed independent associations with sleep trouble and sleep disorders. Moreover, the relationships between OBS and both sleep disorders and trouble were influenced by albumin, γ-glutamyl transferase, total bilirubin, and white blood cells, with combined mediation effects of 34.66 and 29.54%, respectively (both p &lt; 0.001). Sensitivity analyses revealed a significant association between OBS and sleep disorder (p &lt; 0.001).ConclusionThis study revealed a positive correlation between an elevated OBS and improved sleep quality, manifested by decreased sleep disorders, mitigated sleep trouble, and prolonged sleep duration. This is potentially mediated by oxidative stress and inflammation. Therefore, the study underscores the importance of adopting a diet rich in antioxidants and healthy lifestyle choices to address sleep-related concerns, providing a novel avenue for enhancing overall sleep quality.
Nutritional diagnoses in people with type 2 diabetes: association with metabolic, anthropometric, and dietary parameters
Authors: Landa-Anell María Victoria , Del Razo-Olvera Fabiola M. , Bodnar Irene , Cordova-Isidro Brenda , Lagunas-Valdepeña Daniela , Arias-Marroquín Ana T. , García-Ulloa Ana Cristina , Melgarejo-Hernández Marco Antonio , Hernández-Jiménez Sergio ,   for the Group of Study CAIPaDi
Published: October 2024

BackgroundNutritional diagnosis involves identifying a nutritional problem, its cause, and the signs that indicate it to guide appropriate treatment. Few studies report on the most prevalent nutritional diagnoses in people living with type 2 diabetes (T2D).ObjectiveTo define nutritional diagnoses across different domains and their association with metabolic, anthropometric, and dietary parameters in individuals with T2D.MethodsA personalized nutritional intervention was conducted using the Nutrition Care Process (NCP) model, which encompasses assessment, diagnosis, intervention, and evaluation, utilizing standardized terminology from the Nutrition Care Process Terminology (NCPT). Two dietitians, trained and standardized in applying the NCP and NCPT, performed patient assessments and established the diagnoses. Patients over 18 years old with a diagnosis of T2D for less than 5 years were included.ResultsData from 2,050 patients were analyzed, of whom 55.3% were women, and 44.7% were men, with a median age of 57 and 54 years, respectively. The most prevalent nutritional diagnosis was excessive energy and carbohydrate intake. Diagnoses were distributed across domains: Intake (55.9%), Behavioral/Environmental (32.7%), Clinical (10.2%), and 1.2% without nutritional diagnosis. Significant intergroup differences were observed in anthropometric variables such as BMI, waist circumference, and body fat percentage (p &lt; 0.05). HbA1c and glucose levels were significantly higher in the Intake and Behavioral groups (p &lt; 0.001). Albumin/creatinine ratio (ACR) was higher in the ingestion group (p = 0.007). Caloric and carbohydrate intake were higher in the ingestion group, while protein and fat intake were higher in the clinical and behavioral groups (p &lt; 0.001).ConclusionNutritional diagnoses in the intake domain, followed by behavioral/ environmental and clinical domains, are highly prevalent in people with T2D and are associated with worse metabolic control, higher BMI, and increased energy and carbohydrate intake. Timely identification of issues in these domains can support targeted nutritional therapy to improve disease management and promote a healthy lifestyle.Clinical trial registrationhttps://clinicaltrials.gov/study/NCT02836808?term=caipadi&amp;rank=2, Identifier (NCT02836808).
Proteomic and transcriptomic analyses identify apo-transcobalamin-II as a biomarker of overall survival in osteosarcoma
Authors: Lacinski Ryan A. , Dziadowicz Sebastian A. , Roth Clark A. , Ma Li , Melemai Vincent K. , Fitzpatrick Brody , Chaharbakhshi Edwin , Heim Tanya , Lohse Ines , Schoedel Karen E. , Hu Gangqing , Llosa Nicolas J. , Weiss Kurt R. , Lindsey Brock A. 
Published: October 2024

BackgroundThe large-scale proteomic platform known as the SomaScan® assay is capable of simultaneously measuring thousands of proteins in patient specimens through next-generation aptamer-based multiplexed technology. While previous studies have utilized patient peripheral blood to suggest serum biomarkers of prognostic or diagnostic value in osteosarcoma (OSA), the most common primary pediatric bone cancer, they have ultimately been limited in the robustness of their analyses. We propose utilizing this aptamer-based technology to describe the systemic proteomic milieu in patients diagnosed with this disease.MethodsTo determine novel biomarkers associated with overall survival in OSA, we deployed the SomaLogic SomaScan® 7k assay to investigate the plasma proteomic profile of naive primary, recurrent, and metastatic OSA patients. Following identification of differentially expressed proteins (DEPs) between 2-year deceased and survivor cohorts, publicly available databases including Survival Genie, TIGER, and KM Plotter Immunotherapy, among others, were utilized to investigate the significance of our proteomic findings.ResultsApo-transcobalamin-II (APO-TCN2) was identified as the most DEP between 2-year deceased and survivor cohorts (Log2 fold change = 6.8, P-value = 0.0017). Survival analysis using the Survival Genie web-based platform indicated that increased intratumoral TCN2 expression was associated with better overall survival in both OSA (TARGET-OS) and sarcoma (TCGA-SARC) datasets. Cell-cell communication analysis using the TIGER database suggested that TCN2+ Myeloid cells likely interact with marginal zone and immunoglobin-producing B lymphocytes expressing the TCN2 receptor (CD320) to promote their proliferation and survival in both non-small cell lung cancer and melanoma tumors. Analysis of publicly available OSA scRNA-sequencing datasets identified similar populations in naive primary tumors. Furthermore, circulating APO-TCN2 levels in OSA were then associated with a plasma proteomic profile likely necessary for robust B lymphocyte proliferation, infiltration, and formation of intratumoral tertiary lymphoid structures for improved anti-tumor immunity.ConclusionsOverall, APO-TCN2, a circulatory protein previously described in various lymphoproliferative disorders, was associated with 2-year survival status in patients diagnosed with OSA. The relevance of this protein and apparent immunological function (anti-tumor B lymphocyte responses) was suggested using publicly available solid tumor RNA-sequencing datasets. Further studies characterizing the biological function of APO-TCN2 and its relevance in these diseases is warranted.
Asiaticoside improves depressive-like behavior in mice with chronic unpredictable mild stress through modulation of the gut microbiota
Authors: Ren Qingyi , He Chenxi , Sun Yuhong , Gao Xiaowei , Zhou Yan , Qin Tao , Zhang Zhuo , Wang Xiaodong , Wang Jun , Wei Siping , Wang Fang 
Published: October 2024

BackgroundAsiaticoside, the main active ingredient of Centella asiatica, is a pentacyclic triterpenoid compound. Previous studies have suggested that asiaticoside possesses neuroprotective and anti-depressive properties, however, the mechanism of its anti-depressant action not fully understood. In recent years, a growing body of research on anti-depressants has focused on the microbiota-gut-brain axis, we noted that disruption of the gut microbial community structure and diversity can induce or exacerbate depression, which plays a key role in the regulation of depression.MethodsBehavioral experiments were conducted to detect depression-like behavior in mice through sucrose preference, forced swimming, and open field tests. Additionally, gut microbial composition and short-chain fatty acid (SCFA) levels in mouse feces were analyzed 16S rRNA sequencing and gas chromatography-mass spectrometry (GC-MS). Hippocampal brain-derived neurotrophic factor (BDNF) and 5-hydroxytryptamine receptor 1A (5-HT1A) expression in mice was assessed by western blotting. Changes in serum levels of inflammatory factors, neurotransmitters, and hormones were measured in mice using ELISA.ResultsThis study revealed that oral administration of asiaticoside significantly improved depression-like behavior in chronic unpredictable mild stress (CUMS) mice. It partially restored the gut microbial community structure in CUMS mice, altered SCFA metabolism, regulated the hypothalamic–pituitary–adrenal axis (HPA axis) and inflammatory factor levels, upregulated BDNF and 5-HT1A receptor protein expression, and increased serum serotonin (5-hydroxytryptamine, 5-HT) concentration. These findings reveal that asiaticoside exerts antidepressant effects via the microbiota-gut-brain axis.ConclusionsThese results suggested that asiaticoside exerts antidepressant effects through the microbiota-gut-brain axis in a CUMS mouse model.
Factors associated with perceived social support among adolescents in Gamo Zone, Southern Ethiopia: a community-based cross-sectional study
Authors: Sidamo Negussie Boti , Kerbo Amene Abebe , Wado Yohannes Dibaba , Koyira Mengistu Meskele , Gidebo Kassa Daka 
Published: October 2024

IntroductionPerceived social support plays a crucial role in adolescent development, health, well-being, and resilience. Understanding the factors associated with perceived social support among adolescents is essential for designing effective interventions. However, research in this area, particularly within specific contexts, remains limited. Therefore, this study aims to identify the factors associated with perceived social support among adolescents in the Gamo Zone, South Ethiopia Regional State.MethodA community-based cross-sectional study was conducted, involving 1172 adolescents selected through a stratified multi-stage sampling method. Structured face-to-face interviews were employed for data collection. Summary statistics were utilized for data presentation. Multiple linear regressions were then performed to identify factors associated with perceived social support. The strength and direction of associations were presented using β coefficients and 95% confidence intervals (CIs). The level of statistical significance was set at alpha 5%.ResultThe total mean for perceived social support among adolescents was 57.02 ± 12.68. Adolescents living with their biological parents reported significantly higher levels of perceived social support (β = 4.17, 95% CI: 2.00 to 6.34) compared to their counterparts. Similarly, adolescents engaged in paid work within the last 12 months reported higher perceived social support (β = 3.43, 95% CI: 1.39 to 5.46). Higher levels of parental monitoring were also associated with increased perceived social support (β = 3.03, 95% CI: 1.23 to 4.82). Additionally, adolescents who perceived risks for sexual and reproductive health (SRH) problems reported higher levels of perceived social support (β = 2.76, 95% CI: 0.84 to 4.69). Moreover, adolescents with good knowledge about SRH rights exhibited increased perceived social support (β = 2.46, 95% CI: 0.89 to 4.02). Furthermore, adolescents residing in rural areas reported higher levels of perceived social support compared to those in urban areas (β = 1.56, 95% CI: 0.16 to 3.11).ConclusionThe findings of this study reveal that factors such as living arrangements, employment status, parental monitoring, perceived risks for SRH problems, knowledge about SRH rights, and geographical context emerged as important predictors of perceived social support. Therefore, implementing interventions and initiatives to address these identified factors holds promise for nurturing resilient adolescent social support networks.
Surgical Hyperspectral imaging and Indocyanine green Near-infrared Examination (SHINE) for brain arteriovenous malformation resection: a case report on how to visualize perfusion
Authors: Wach Johannes , Weber Ferdinand , Vychopen Martin , Arlt Felix , Pfahl Annekatrin , Köhler Hannes , Melzer Andreas , Güresir Erdem 
Published: October 2024

Background and importanceArteriovenous malformations (AVMs) are complex vascular anomalies that pose significant risks, including intracranial hemorrhage and neurological deficits. Surgical resection is the preferred treatment, requiring precise intraoperative imaging to ensure complete removal while preserving critical structures. This case report presents the first combined use of hyperspectral imaging (HSI) and indocyanine green video angiography (ICG VA) to visualize perfusion during brain AVM surgery, highlighting the potential benefits of these advanced imaging techniques.Case descriptionA 66-year-old male presented with chronic headaches but no neurological deficits. MRI revealed a superficial AVM in the left frontal lobe within the superior frontal sulcus, measuring approximately 2.4 cm. The AVM was fed by feeders from the pericallosal artery, callosomarginal artery, and middle cerebral artery (MCA) branches, with drainage through a dilated cortical vein into the superior sagittal sinus. Preoperative embolization of two MCA feeding branches was performed, followed by microsurgical resection with ICG VA and HSI.ConclusionsThis case report demonstrates the successful application of HSI and ICG VA in brain AVM surgery. The combined use of these technologies provided comprehensive intraoperative assessment, enhancing surgical precision and safety. The integration of HSI offers non-invasive, contrast-agent-free imaging, potentially improving outcomes by enabling detailed perfusion mapping. Future studies should explore the broader applications of these imaging modalities in neurovascular practice.
In situ assessment of neuroinflammatory cytokines in different stages of ovine natural prion disease
Authors: Guijarro Isabel M. , Garcés Moisés , Badiola Juan J. , Monzón Marta 
Published: October 2024

IntroductionAccording to the neuroinflammatory hypothesis, a cytokine-mediated host innate immune response may be involved in the mechanisms that contribute to the process of neurodegeneration. Specifically, regarding prion diseases, some experimental murine models have evidenced an altered profile of inflammatory intermediaries. However, the local inflammatory response has rarely been assessed, and never in tissues from different natural models throughout the progression of neurodegeneration.MethodsThe aim of this study was to use immunohistochemistry (IHC) to in situ assess the temporal protein expression of several cytokines in the cerebellum of sheep suffering from various clinical stages of scrapie.Results and discussionClear changes in the expression of most of the assessed markers were observed in the affected sheep compared to the healthy control sheep, and from different stages. In summary, this preliminary IHC study focusing in the Purkinje cell layer changes demonstrate that all cytokines or respective receptors studied (IL-1, IL-1R, IL-2R, IL-6, IL-10R, and TNFαR) except for IFNγR are disease-associated signaling proteins showing an increase or decrease in relation to the progression of clinical disease. In the future, this study will be extended to other inflammatory mediators and brain regions, focusing in particular on the release of these inflammatory mediators by astroglial and microglial populations.