bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs
Authors: Meader Ellie , Barta Tomas , Melguizo-Sanchis Dario , Tilgner Katarzyna , Montaner David , El-Harouni Ashraf A. , Armstrong Lyle , Lako Majlinda 
Published: October 2017

Abstract
Hematopoietic stem cells derived from pluripotent stem cells could be used as an alternative to bone marrow transplants. Deriving these has been a long-term goal for researchers. However, the success of these efforts has been limited with the cells produced able to engraft in the bone marrow of recipient animals only in very low numbers. There is evidence that defects in the migratory and homing capacity of the cells are due to mis-regulation of miRNA expression and are responsible for their failure to engraft. We compared the miRNA expression profile of hematopoietic progenitors derived from pluripotent stem cells to those derived from bone marrow and found that numerous miRNAs are too highly expressed in hematopoietic progenitors derived from pluripotent stem cells, and that most of these are inhibitors of epithelial-mesenchymal transition or metastasis (including miR-200b, miR-200c, miR-205, miR-148a, and miR-424). We hypothesize that the high expression of these factors, which promote an adherent phenotype, may be causing the defect in hematopoietic differentiation. However, inhibiting these miRNAs, individually or in multiplex, was insufficient to improve hematopoietic differentiation in vitro, suggesting that other miRNAs and/or genes may be involved in this process.
Study protocol of the Intense Physical Activity and Cognition study: The effect of high‐intensity exercise training on cognitive function in older adults
Authors: Brown Belinda M. , Rainey‐Smith Stephanie R. , Castalanelli Natalie , Gordon Nicole , Markovic Shaun , Sohrabi Hamid R. , Weinborn Michael , Laws Simon M. , Doecke James , Shen Kaikai , Martins Ralph N. , Peiffer Jeremiah J. 
Published: October 2017

AbstractIntroductionInconsistent results from previous studies of exercise and cognitive function suggest that rigorously designed randomized controlled trials are urgently needed. Here, we describe the design of the Intense Physical Activity and Cognition (IPAC) study, which will assess the impact of a 6‐month high‐intensity exercise intervention on cognitive function and biomarkers of dementia risk, compared with a 6‐month moderate‐intensity exercise intervention and control group (no study‐related exercise).MethodsOne‐hundred and five cognitively healthy men and women aged between 60 and 80 years are randomized into a high‐intensity exercise, moderate‐intensity exercise, or control group. Individuals randomized to an exercise intervention undertake 6 months of cycle‐based exercise twice a week, at 50 minutes per session. All participants undergo comprehensive neuropsychological testing, blood sampling, brain magnetic resonance imaging, fitness testing, and a body composition scan at baseline, 6 months (immediately after intervention), and 18 months (12 months after intervention).DiscussionThe IPAC study takes a multidisciplinary approach to investigating the role of exercise in maintaining a healthy brain throughout aging. Rigorous monitoring of exertion and adherence throughout the intervention, combined with repeated measures of fitness, is vital in ensuring an optimum exercise dose is reached. Results from the IPAC study will be used to inform a large‐scale multicentre randomized controlled trial, with the ultimate aim of pinpointing the frequency, duration, and intensity of exercise that provides the most benefit to the brain, in terms of enhancing cognitive function and reducing dementia risk in older adults.
HISTORY IN TWENTIETH-CENTURY ETHIOPIA: THE ‘GREAT TRADITION’ AND THE COUNTER-HISTORIES OF NATIONAL FAILURE
Authors: MARZAGORA SARA 
Published: October 2017

AbstractDrawing from both fictional and non-fictional sources, this article traces the way history was conceptualised in twentieth-century Ethiopia by secular educated elites, charting the changing power relations between Ethiopia's hegemonic historiographical paradigm and the alternative historical visions that challenged this ‘Great Tradition’ over the course of the century. While the Great Tradition extols Ethiopia's past and future glories, the counter-histories focused instead on the country's failure to develop and democratise. Against the interpretation that the counter-histories supplanted the Great Tradition in the late 1960s, the article examines them in terms of complementarity. The intellectual interventions of young student radicals in the late 1960s constitute a break, but not a drastic paradigm shift from the past. The Great Tradition had already been called into question by older generations of intellectuals, even if they proved unable or unwilling to translate their disillusionment into political action.
THE COLONIAL PASTS OF MEDIEVAL TEXTS IN NORTHERN AFRICA: USEFUL KNOWLEDGE, PUBLICATION HISTORY, AND POLITICAL VIOLENCE IN COLONIAL AND POST-INDEPENDENCE ALGERIA
Authors: LOVE PAUL M. 
Published: October 2017

AbstractThis article argues that medieval Arabic texts that were published in colonial northern Africa constitute as much a part of the history of colonialism and its legacy as that of the medieval centuries in which they were written. Using the publication history of a medieval Ibadi text and its French translations, I demonstrate how texts like it were edited, translated, and published not only for academic purposes, but also as contributions to the production of ‘useful’ colonial knowledge in Algeria. I begin with the first translation, published in 1878 alongside other ethnographic and historical studies funded by the colonial state. I then turn to the second translation, serially published between 1960–2 as its editors abandoned the country at the violent end of the colonial period. Finally, I address the Arabic editions published after independence, which recast it within a nationalist framework. Overall, I argue for the importance of addressing the colonial pasts of medieval texts in northern Africa.
Edmund Burke on the Question of Commercial Intercourse in the Eighteenth Century
Authors: Collins Gregory M. 
Published: October 2017

AbstractScholars have noticed that Edmund Burke's impassioned economic tract in favor of market liberty,Thoughts and Details on Scarcity, appears to be at odds with his political philosophy and rhetorical temperament of prudence and restraint. This essay challenges this interpretation. I contend that Burke's emotional statements in the writing reflect strong continuities with his earlier reflections and political activities regarding economic issues. From his earliest days in Parliament to his final years, Burke was a firm supporter of commercial liberty, both domestic and foreign. I conclude by arguing that we cannot properly understand Burke's belief in gradual reform unless we grasp his idea of incremental commercial improvement.
Phosphorylable tyrosine residue 162 in the double-stranded RNA-dependent kinase PKR modulates its interaction with SUMO
Authors: de la Cruz-Herrera Carlos F. , Baz-Martínez Maite , Motiam Ahmed El , Vidal Santiago , Collado Manuel , Vidal Anxo , Rodríguez Manuel S. , Esteban Mariano , Rivas Carmen 
Published: October 2017

AbstractActivated dsRNA-dependent serine/threonine kinase PKR phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), resulting in a shut-off of general translation, induction of apoptosis, and inhibition of virus replication. PKR can be activated by binding to dsRNA or cellular proteins such as PACT/RAX, or by its conjugation to ISG15 or SUMO. Here, we demonstrate that PKR also interacts with SUMO in a non-covalent manner. We identify the phosphorylable tyrosine residue 162 in PKR (Y162) as a modulator of the PKR-SUMO non-covalent interaction as well as of the PKR SUMOylation. Finally, we show that the efficient SUMO-mediated eIF2α phosphorylation and inhibition of protein synthesis induced by PKR in response to dsRNA depend on this residue. In summary, our data identify a new mechanism of regulation of PKR activity and reinforce the relevance of both, tyrosine phosphorylation and SUMO interaction in controlling the activity of PKR.
Compressive Volumetric Light-Field Excitation
Authors: Schedl David C. , Bimber Oliver 
Published: October 2017

AbstractWe explain how volumetric light-field excitation can be converted to a process that entirely avoids 3D reconstruction, deconvolution, and calibration of optical elements while taking scattering in the probe better into account. For spatially static probes, this is achieved by an efficient (one-time) light-transport sampling and light-field factorization. Individual probe particles (and arbitrary combinations thereof) can subsequently be excited in a dynamically controlled way while still supporting volumetric reconstruction of the entire probe in real-time based on a single light-field recording.
Transcriptomic response to thermal and salinity stress in introduced and native sympatric Palaemon caridean shrimps
Authors: Marie Amandine D. , Smith Steve , Green Andy J. , Rico Ciro , Lejeusne Christophe 
Published: October 2017

AbstractOrganisms develop local adaptations to cope with spatially and temporally variable environments such as estuarine habitats, where abiotic parameters such as salinity and temperature fluctuate continuously. Studying the regulation of gene expression in a variable environment allows us to understand the underlying molecular mechanisms of these adaptations and the relative roles of the genetic and plastic response. The transcriptomes of the European native Palaemon longirostris (PL) and the introduced P. macrodactylus (PM) shrimps are described and compared after an experiment simulating summer conditions in the Guadalquivir Estuary, Spain. Specimens, collected in the Guadalquivir Estuary, were maintained at a temperature and salinity of 20 °C and 5 ppt for the control, and 30 °C and 15 ppt for the stress treatment. A large amount of differential gene expression was observed: 16,013 and 2,594 for PL and PM respectively. Functionally annotated unigenes revealed some differences, with PL seemingly having to face stronger physiological stress than PM. Thus, PM seems to have greater resistance than PL under conditions of high temperature and salinity. These results constitute a step forward in the understanding of the underlying molecular mechanisms of genetic adaptation of native invertebrates, and alien taxa that have successfully invaded estuaries in temperate regions around the world.
Cellular splicing factor UAP56 stimulates trimeric NP formation for assembly of functional influenza viral ribonucleoprotein complexes
Authors: Hu Yifan , Gor Vishal , Morikawa Kazuya , Nagata Kyosuke , Kawaguchi Atsushi 
Published: October 2017

AbstractThe influenza virus RNA genome exists as a ribonucleoprotein (RNP) complex by interacting with NP, one of virus-encoded RNA binding proteins. It is proposed that trimeric NP is a functional form, but it is not clear how trimeric NP is formed and transferred to RNA. UAP56, a cellular splicing factor, functions as a molecular chaperone for NP and is required for the replication-coupled RNP formation of newly synthesized viral genome, but the details of NP transfer to viral RNA by UAP56 is unclear. Here we found that UAP56 is complexed with trimeric NP, but not monomeric NP. Gel filtration analysis and atomic force microscopy analysis indicated that the complex consists of two trimeric NP connected by UAP56. We also found that UAP56 stimulates trimeric NP formation from monomeric NP even at physiological salt concentrations. Thus, UAP56 facilitates the transfer of NP to viral RNA since trimeric NP has higher RNA binding activity than monomeric NP. Further, UAP56 represses the binding of excess amount of NP to RNA possibly by transferring trimeric NP. Collectively, we propose that UAP56 stimulates viral RNP formation through promotion of the assembly of trimeric NP and is important for the structural integrity of NP-RNA complex.
Tuning methane decomposition on stepped Ni surface: The role of subsurface atoms in catalyst design
Authors: Arevalo Ryan Lacdao , Aspera Susan Meñez , Escaño Mary Clare Sison , Nakanishi Hiroshi , Kasai Hideaki 
Published: October 2017

AbstractThe decomposition of methane (CH4) is a catalytically important reaction in the production of syngas that is used to make a wide spectrum of hydrocarbons and alcohols, and a principal carbon deposition pathway in methane reforming. Literatures suggest that stepped Ni surface is uniquely selective toward methane decomposition to atomic C, contrary to other catalysts that favor the CH fragment. In this paper, we used dispersion-corrected density functional theory-based first principles calculations to identify the electronic factors that govern this interesting property of stepped Ni surface. We found that the adsorption of atomic C on this surface is uniquely characterized by a 5–coordinated bonding of C with Ni atoms from both the surface and subsurface layers. Comparison with Ru surface indicates the importance of the subsurface atoms of stepped Ni surface on its selectivity toward methane decomposition to atomic C. Interestingly, we found that substituting these subsurface atoms with other elements can dramatically change the reaction mechanism of methane decomposition, suggesting a new approach to catalyst design for hydrocarbon reforming applications.
PARP1-produced poly-ADP-ribose causes the PARP12 translocation to stress granules and impairment of Golgi complex functions
Authors: Catara Giuliana , Grimaldi Giovanna , Schembri Laura , Spano Daniela , Turacchio Gabriele , Lo Monte Matteo , Beccari Andrea Rosario , Valente Carmen , Corda Daniela 
Published: October 2017

AbstractPoly-ADP-ribose-polymerases (PARPs) 1 and 2 are nuclear enzymes that catalyze the poly-ADP-ribosylation of nuclear proteins transferring poly-ADP-ribose (PAR) polymers to specific residues. PARPs and PAR intervene in diverse functions, including DNA repair in the nucleus and stress granule assembly in the cytoplasm. Stress granules contribute to the regulation of translation by clustering and stabilizing mRNAs as well as several cytosolic PARPs and signaling proteins to modulate cell metabolism and survival. Our study is focused on one of these PARPs, PARP12, a Golgi-localized mono-ADP-ribosyltransferase that under stress challenge reversibly translocates from the Golgi complex to stress granules. PARP1 activation and release of nuclear PAR drive this translocation by direct PAR binding to the PARP12-WWE domain. Thus, PAR formation functionally links the activity of the nuclear and cytosolic PARPs during stress response, determining the release of PARP12 from the Golgi complex and the disassembly of the Golgi membranes, followed by a block in anterograde-membrane traffic. Notably, these functions can be rescued by reverting the stress condition (by drug wash-out). Altogether these data point at a novel, reversible nuclear signaling that senses stress to then act on cytosolic PARP12, which in turn converts the stress response into a reversible block in intracellular-membrane traffic.
Telmisartan Activates PPARδ to Improve Symptoms of Unpredictable Chronic Mild Stress-Induced Depression in Mice
Authors: Li Yingxiao , Cheng Kai-Chun , Liu Keng-Fan , Peng Wen-Huang , Cheng Juei-Tang , Niu Ho-Shan 
Published: October 2017

AbstractMajor depression is a common mental disorder that has been established to be associated with a decrease in serotonin and/or serotonin transporters in the brain. Peroxisome proliferator-activated receptor δ (PPARδ) has been introduced as a potential target for depression treatment. Telmisartan was recently shown to activate PPARδ expression; therefore, the effectiveness of telmisartan in treating depression was investigated. In unpredictable chronic mild stress (UCMS) model, treatment with telmisartan for five weeks notably decrease in the time spent in the central and the reduced frequency of grooming and rearing in open filed test (OFT) and the decreased sucrose consumption in sucrose preference test (SPT) compared with the paradigms. Telmisartan also reversed the decrease in PPARδ and 5-HTT levels in the hippocampus of depression-like mice. Administration of PPARδ antagonist GSK0660 and direct infusion of sh-PPARδ into the brain blocked the effects of telmisartan on the improvement of depression-like behavior in these mice. Moreover, telmisartan enhanced the expression of PPARδ and 5HTT in H19-7 cells. In conclusion, the obtained results suggest that telmisartan improves symptoms of stress-induced depression in animals under chronic stress through activation of PPARδ. Therefore, telmisartan may be developed as a potential anti-depressant in the future.
Observed drought indices show increasing divergence across Europe
Authors: Stagge James H. , Kingston Daniel G. , Tallaksen Lena M. , Hannah David M. 
Published: October 2017

AbstractRecent severe European droughts raise the vital question: are we already experiencing measurable changes in drought likelihood that agree with climate change projections? The plethora of drought definitions compounds this question, requiring instead that we ask: how have various types of drought changed, how do these changes compare with climate projections, and what are the causes of observed differences? To our knowledge, this study is the first to reveal a regional divergence in drought likelihood as measured by the two most prominent meteorological drought indices: the Standardized Precipitation Index (SPI) and the Standardized Precipitation-Evapotranspiration Index (SPEI) across Europe over the period 1958–2014. This divergence is driven primarily by an increase in temperature from 1970–2014, which in turn increased reference evapotranspiration (ET0) and thereby drought area measured by the SPEI. For both indices, Europe-wide analysis shows increasing drought frequencies in southern Europe and decreasing frequencies in northern Europe. Notably, increases in temperature and ET0 have enhanced droughts in southern Europe while counteracting increased precipitation in northern Europe. This is consistent with projections under climate change, indicating that climate change impacts on European drought may already be observable and highlighting the potential for discrepancies among standardized drought indices in a non-stationary climate.
Unveiling the physical mechanism behind pistol shrimp cavitation
Authors: Koukouvinis Phoevos , Bruecker Christoph , Gavaises Manolis 
Published: October 2017

AbstractSnapping shrimps use a special shaped claw to generate a cavitating high speed water jet. Cavitation formed in this way, may be used for hunting/stunning prey and communication. The present work is a novel computational effort to provide insight on the mechanisms of cavitation formation during the claw closure. The geometry of the claw used here is a simplified claw model, based on prior experimental work. Techniques, such as Immersed Boundary and Homogenous Equilibrium Model (HEM), are employed to describe the claw motion and cavitating flow field respectively. The simulation methodology has been validated against prior experimental work and is applied here for claw closure at realistic conditions. Simulations show that during claw closure, a high velocity jet forms, inducing vortex roll-up around it. If the closure speed is high enough, the intensity of the swirling motion is enough to produce strong depressurization in the vortex core, leading to the formation of a cavitation ring. The cavitation ring moves along the jet axis and, soon after its formation, collapses and rebounds, producing high pressure pulses.
Yucasin DF, a potent and persistent inhibitor of auxin biosynthesis in plants
Authors: Tsugafune Shinichi , Mashiguchi Kiyoshi , Fukui Kosuke , Takebayashi Yumiko , Nishimura Takeshi , Sakai Tatsuya , Shimada Yukihisa , Kasahara Hiroyuki , Koshiba Tomokazu , Hayashi Ken-ichiro 
Published: October 2017

AbstractThe plant hormone auxin plays a crucial role in plant growth and development. Indole-3-acetic acid (IAA), a natural auxin, is mainly biosynthesized by two sequential enzyme reactions catalyzed by TAA1 and YUCCA (YUC). TAA1 is involved in the conversion of tryptophan to IPA, and YUC catalyzes the conversion of IPA to IAA. We previously demonstrated that yucasin inhibits AtYUC1 enzyme activity and suppress high-auxin phenotype of YUC overexpression plants, although yucasin displayed weak effects on the auxin-related phenotype of wild-type plants. To develop more potent YUC inhibitors, various derivatives of yucasin were synthesized, and their structure–activity relationships were investigated. Yucasin difluorinated analog (YDF) (5-[2,6-difluorophenyl]-2,4-dihydro-[1,2,4]-triazole-3-thione) was identified to be a more potent YUC inhibitor than the original yucasin. YDF caused an auxin-deficient phenotype in Arabidopsis wild-type plants that was restored with auxin application. YDF was found to be highly stable regarding metabolic conversion in vivo, accounting for the potent activity of the inhibition of IAA biosynthesis in planta. Photoaffinity labeling experiments demonstrated that yucasin-type inhibitors bind to the active site of AtYUC1. YDF is a promising auxin biosynthesis inhibitor and is a useful chemical tool for plant biology and agrochemical studies.
A simple surface plasmon resonance biosensor for detection of PML/RARα based on heterogeneous fusion gene-triggered nonlinear hybridization chain reaction
Authors: Guo Bin , Cheng Wei , Xu Yongjie , Zhou Xiaoyan , Li Xinmin , Ding Xiaojuan , Ding Shijia 
Published: October 2017

AbstractIn this work, a simple and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of two major PML/RARα (promyelocytic leukemia, retinoic acid receptor alpha) subtypes based on the heterogeneous fusion gene-triggered nonlinear hybridization chain reaction (HCR). On the gold chip surface, the cascade self-assembly process is triggered after the introduction of PML/RARα. The different fragments of PML/RARα can specifically hybridize with capture probes (Cp) immobilized on the chip and the hybridization DNA1 (H1). Then, the nonlinear HCR is initiated by the complex of Cp-PML/RARα-H1 with the introduction of two hybridization DNA chains (H2 and H3). As a result, a dendritic nanostructure is achieved on the surface of chip, leading to a significant SPR amplification signal owing to its high molecular weight. The developed method shows good specificity and high sensitivity with detection limit of 0.72 pM for “L” subtype and 0.65 pM for “S” subtype. Moreover, this method has been successfully applied for efficient identification of clinical positive and negative PCR samples of the PML/RARα subtype. Thus, this developed biosensing strategy presents a potential platform for analysis of fusion gene and early diagnosis of clinical disease.
New Routes for Refinery of Biogenic Platform Chemicals Catalyzed by Cerium Oxide-supported Ruthenium Nanoparticles in Water
Authors: Mizugaki Tomoo , Togo Keito , Maeno Zen , Mitsudome Takato , Jitsukawa Koichiro , Kaneda Kiyotomi 
Published: October 2017

AbstractHighly selective hydrogenative carbon–carbon bond scission of biomass-derived platform oxygenates was achieved with a cerium oxide-supported ruthenium nanoparticle catalyst in water. The present catalyst enabled the selective cleavage of carbon–carbon σ bonds adjacent to carboxyl, ester, and hydroxymethyl groups, opening new eight synthetic routes to valuable chemicals from biomass derivatives. The high selectivity for such carbon-carbon bond scission over carbon-oxygen bonds was attributed to the multiple catalytic roles of the Ru nanoparticles assisted by the in situ formed Ce(OH)3.