bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of LOC285758, a potential long non-coding biomarker, is methylation-dependent and correlates with glioma malignancy grade
Authors: Matjasic Alenka , Popovic Mara , Matos Bostjan , Glavac Damjan 
Published: January 2017

AbstractBackgroundIdentifying the early genetic drivers can help diagnose glioma tumours in their early stages, before becoming malignant. However, there is emerging evidence that disturbance of epigenetic mechanisms also contributes to cell’s malignant transformation and cancer progression. Long non-coding RNAs are one of key epigenetic modulators of signalling pathways, since gene expression regulation is one of their canonical mechanisms. The aim of our study was to search new gliomagenesis-specific candidate lncRNAs involved in epigenetic regulation.Patients and methodsWe used a microarray approach to detect expression profiles of epigenetically involved lncRNAs on a set of 12 glioma samples, and selectedLOC285758for further qPCR expression validation on 157 glioma samples of different subtypes. To establish if change in expression is a consequence of epigenetic alterations we determined methylation status of lncRNA’s promoter using MS-HRM. Additionally, we used the MLPA analysis for determining the status of known glioma biomarkers and used them for association analyses.ResultsIn all glioma subtypes levels of LOC285758 were significantly higher in comparison to normal brain reference RNA, and expression was inversely associated with promoter methylation. Expression substantially differs between astrocytoma and oligodendroglioma, and is elevated in higher WHO grades, which also showed loss of methylation.ConclusionsOur study revealed that lncRNALOC285758changed expression in glioma is methylation-dependent and methylation correlates with WHO malignancy grade. Methylation is also distinctive between astrocytoma I-III and other glioma subtypes and may thus serve as an additional biomarker in glioma diagnosis.
Optimal planning strategy among various arc arrangements for prostate stereotactic body radiotherapy with volumetric modulated arc therapy technique
Authors: Kang Sang Won , Chung Jin Beom , Kim Jae Sung , Kim In Ah , Eom Keun Yong , Song Changhoon , Lee Jeong Woo , Kim Jin Young , Suh Tae Suk 
Published: January 2017

Abstract

Background
The aim of this study was to determine the optimal strategy among various arc arrangements in prostate plans of stereotactic body radiotherapy with volumetric modulated arc therapy (SBRT-VMAT).

Patients and methods
To investigate how arc arrangements affect dosimetric and biological metrics, SBRT-VMAT plans for eighteen patients were generated with arrangements of single-full arc (1FA), single-partial arc (1PA), double-full arc (2FA), and double-partial arc (2PA). All plans were calculated by the Acuros XB calculation algorithm. Dosimetric and radiobiological metrics for target volumes and organs at risk (OARs) were evaluated from dosevolume histograms.

Results
All plans were highly conformal (CI<1.05, CN=0.91) and homogeneous (HI=0.09-0.12) for target volumes. For OARs, there was no difference in the bladder dose, while there was a significant difference in the rectum and both femoral head doses. Plans using 1PA and 2PA showed a strong reduction to the mean rectum dose compared to plans using 1FA and 2FA. Contrastively, the D2% and mean dose in both femoral heads were always lower in plans using 1FA and 2FA. The average tumor control probability and normal tissue complication probability were comparable in plans using all arc arrangements.

Conclusions
The use of 1PA had a more effective delivery time and produced equivalent target coverage with better rectal sparing, although all plans using four arc arrangements showed generally similar for dosimetric and biological metrics. However, the D2% and mean dose in femoral heads increased slightly and remained within the tolerance. Therefore, this study suggests that the use of 1PA is an attractive choice for delivering prostate SBRT-VMAT.
Circadian Kinetics of Cell Cycle Progression in Adult Neurogenic Niches of a Diurnal Vertebrate
Authors: Akle Veronica , Stankiewicz Alexander J. , Kharchenko Vasili , Yu Lili , Kharchenko Peter V. , Zhdanova Irina V. 
Published: January 2017

The circadian system may regulate adult neurogenesis via intracellular molecular clock mechanisms or by modifying the environment of neurogenic niches, with daily variation in growth factors or nutrients depending on the animal's diurnal or nocturnal lifestyle. In a diurnal vertebrate, zebrafish, we studied circadian distribution of immunohistochemical markers of the cell division cycle (CDC) in 5 of the 16 neurogenic niches of adult brain, the dorsal telencephalon, habenula, preoptic area, hypothalamus, and cerebellum. We find that common to all niches is the morning initiation of G1/S transition and daytime S-phase progression, overnight increase in G2/M, and cycle completion by late night. This is supported by the timing of gene expression for critical cell cycle regulators cyclins D, A2, and B2 and cyclin-dependent kinase inhibitor p20 in brain tissue. The early-night peak inp20, limiting G1/S transition, and its phase angle with the expression of core clock genes,Clock1andPer1, are preserved in constant darkness, suggesting intrinsic circadian patterns of cell cycle progression. The statistical modeling of CDC kinetics reveals the significant circadian variation in cell proliferation rates across all of the examined niches, but interniche differences in the magnitude of circadian variation in CDC, S-phase length, phase angle of entrainment to light or clock, and its dispersion. We conclude that, in neurogenic niches of an adult diurnal vertebrate, the circadian modulation of cell cycle progression involves both systemic and niche-specific factors.SIGNIFICANCE STATEMENTThis study establishes that in neurogenic niches of an adult diurnal vertebrate, the cell cycle progression displays a robust circadian pattern. Common to neurogenic niches located in diverse brain regions is daytime progression of DNA replication and nighttime mitosis, suggesting systemic regulation. Differences between neurogenic niches in the phase and degree of S-phase entrainment to the clock suggest additional roles for niche-specific regulatory mechanisms. Understanding the circadian regulation of adult neurogenesis can help optimize the timing of therapeutic approaches in patients with brain traumas or neurodegenerative disorders and preserve neural stem cells during cytostatic cancer therapies.
Accumulation of Polyribosomes in Dendritic Spine Heads, But Not Bases and Necks, during Memory Consolidation Depends on Cap-Dependent Translation Initiation
Authors: Ostroff Linnaea E. , Botsford Benjamin , Gindina Sofya , Cowansage Kiriana K. , LeDoux Joseph E. , Klann Eric , Hoeffer Charles 
Published: January 2017

Translation in dendrites is believed to support synaptic changes during memory consolidation. Although translational control mechanisms are fundamental mediators of memory, little is known about their role in local translation. We previously found that polyribosomes accumulate in dendritic spines of the adult rat lateral amygdala (LA) during consolidation of aversive pavlovian conditioning and that this memory requires cap-dependent initiation, a primary point of translational control in eukaryotic cells. Here we used serial electron microscopy reconstructions to quantify polyribosomes in LA dendrites when consolidation was blocked by the cap-dependent initiation inhibitor 4EGI-1. We found that 4EGI-1 depleted polyribosomes in dendritic shafts and selectively prevented their upregulation in spine heads, but not bases and necks, during consolidation. Cap-independent upregulation was specific to spines with small, astrocyte-associated synapses. Our results reveal that cap-dependent initiation is involved in local translation during learning and that local translational control varies with synapse type.SIGNIFICANCE STATEMENTTranslation initiation is a central regulator of long-term memory formation. Local translation in dendrites supports memory by providing necessary proteins at synaptic sites, but it is unknown whether this requires initiation or bypasses it. We used serial electron microscopy reconstructions to examine polyribosomes in dendrites when memory formation was blocked by an inhibitor of translation initiation. This revealed two major pools of polyribosomes that were upregulated during memory formation: one pool in dendritic spine heads that was initiation dependent and another pool in the bases and necks of small spines that was initiation independent. Thus, translation regulation differs between spine types and locations, and translation that occurs closest to individual synapses during memory formation is initiation dependent.
Economía urbana y economia social. Un reconocimiento pendiente
Authors: Muñoz Ruth 
Published: January 2017

La economía urbana (EU) surgió de la mano de propuestas espacialistas y economicismos y continúa siendo tallada por estas A pesar de una evidente institucionalización de la economía social y solidaria (ESS) en América Latina, que da cuenta de un creciente pluralismo económico, en la EU perdura la hegemonía del enfoque del sector informal urbano y sus presiones por transformar en capitalistas cada práctica de economía popular. Buscando el reconocimiento de la economía social (ES) en este campo, el artículo ilustra brevemente la institucionalización de la ESS y plantea una provocativa exploración por poner en diálogo a la ES con la teoría de los dos circuitos. Un diálogo como este constituye una deuda pendiente que puede aportar a la disputa del campo así como a encontrar mejores comprensiones y, también, a transformar las economías de nuestros territorios en un sentido progresivo.Palabras-clave: economía urbana; dos circuitos de la economía urbana; economía social; economía social y solidaria; economía popular. Abstract: The urban economy has been hardly influenced by spatial proposals and economicism. In Latin America, while the institutionalization of social and solidarity economy of the last few years shows a growing economic pluralism, in the urban economy field persists the hegemony of urban informal sector perspective, and its pressures of transforming in capitalist every popular economy practice. Seeking for recognition of social economy in this field, the article succinctly illustrates social and solidarity institutionalization in the region and suggests a provocative exploration to put in dialogue social economy with two circuits of urban economy theory. Such a dialogue is considered as a debt, which we understand could contribute to dispute the field, to get better explanations and also to transform in a progressive sense the economy of our territories. Keywords: urban: economy; two circuits of urban economy; social economy; social and solidarity economy; popular economy.
O Programa Minha Casa Minha Vida na metrópole paulistana: atendimento habitacional e padrões de segregação
Authors: Marques Eduardo , Rodrigues Leandro 
Published: January 2017

Este artigo analisa a produção do programa Minha Casa Minha Vida na região metropolitana de São Paulo. O trabalho parte de uma revisão das principais características e críticas ao programa para investigar o volume de produção, sua adequação à demanda habitacional para cada faixa de renda na região, assim como a localização dos empreendimentos. Utilizando técnicas de geoprocessamento, são levantados os padrões de segregação dos empreendimentos com relação a distâncias a centralidades, a equipamentos públicos e aos conjuntos produzidos pelas políticas habitacionais prévias. Os dados confirmam análises anteriores com relação à localização periférica dos empreendimentos, em especial para a primeira faixa de renda. Entretanto, considerando os padrões de localização dos conjuntos existentes e faixas de renda comparáveis, os resultados sugerem que o programa tem produzido conjuntos menos isolados do que as políticas prévias, não sendo possível afirmar que o programa apresente os mesmos resultados territoriais que as políticas precedentes. Palavras-chave: política habitacional; Minha Casa Minha Vida; segregação; periferia; São Paulo. Abstract: This article analyzes the production of the Minha Casa Minha Vida program at the metropolitan region of São Paulo. Departing from a review of the main features of the program and the criticisms that have been raised against it, the study investigates its production, its adequacy to the housing demand for each income band at the region, as well as the location of the projects. Using GIS techniques, we study the segregation patterns of the projects considering their distances to centralities, to public equipment and to the housing projects produced by previous policies. The data confirm previous analyses which state the peripheral location of the projects, in special for the first income band. However, considering the location of the existing projects for comparable income bands, the results suggest that the program produced less isolated projects than previous policies, not being accurate to state that the program presents the same territorial results than the preceding policies. Keywords: housing policy; Minha Casa Minha Vida; residential segregation; urban periphery; São Paulo.
Políticas sociais e políticas de cultura: territórios e privatizações cruzadas
Authors: Rizek Cibele Saliba 
Published: January 2017

O artigo se baseia em pesquisa que apontou para novas formas de captação de recursos por meio da promoção de práticas culturais que se interligam à gestão de serviços públicos de saúde na Zona Leste da Cidade de São Paulo, sob a direção de organizações sociais privadas. O cruzamento entre modos de captação, gestão terceirizada da cultura e equipamentos de saúde aponta para uma intersetorialidade inédita dessas práticas, o que configura o que poderia ser identificado como um planejamento social privado, redesenhando formas de atuação e margens do Estado por meio de um conjunto de relações entre os programas sociais e a população em condições de pobreza na maior cidade brasileira. Os bairros da última periferia Leste da cidade de São Paulo conformam assim um terreno de experimentações dessas práticas cruzadas para além das caracterizações clássicas das zonas periféricas das grandes metrópoles brasileiras que apontavam para a precariedade das condições de vida bem como para o nascedouro de movimentos sociais, suas demandas, sujeitos e linguagens de direitos, tal como foram percebidos e enunciados a partir do final dos anos oitenta do século XX. Palavras-chave: políticas sociais; políticas públicas; privatização; periferias urbanas. Abstract: This article is based on research that pointed to new ways of raising funds through the promotion of cultural practices that are interconnected to the management of public health services in the Eastern Zone of the City of São Paulo, under the direction of private social organizations. The cross between fundraising, outsourced management of culture and health equipment points to an unprecedented relationship between the sectors of these practices, which sets up what can be identified as a private social planning. This process redrew the margins of the State redefining the relationship between social programs and policies and poor population in the largest Brazilian city. The peripheral neighborhoods of the Eastern outskirts São Paulo became an experimental field of these practices, more than classical character of precarious forms of life or the place of origin of social movements, demands, subjects, right languages, as they were known specially from the 80´s Brazilian sociological literature. Keywords: social policy; public policies; privatization; urban peripheries.
A produção associada autogestionária na construção civil como estratégia para a integração do sistema da economia social na autogestão territorial
Authors: Bickel Cristiano Gurgel 
Published: January 2017

Neste trabalho problematiza-se o segmento autogestionário da construção civil como um lugar teórico-prático de transformação socioeconômica para a superação da produção capitalista do espaço. Para tanto, formulam-se críticas à cultura produtiva, em suas formas heterogestionária e autogestionária, estabelecendo-se proposições teórico-práticas para um amplo funcionamento da economia social no setor da construção civil. Essas proposições estão sintetizadas nos conceitos de canteiro-escola para autogestão e redes de construção autogestionária, enfocando-se respectivamente a reprodução sociopolítica do trabalho associado na construção civil e a reprodução socioeconômica da produção associada em autogestão no território. Dessa forma, por meio da atuação integrada de cooperativas de trabalho, produção e consumo nos segmentos imobiliário, infraestrutura e serviços de construção civil, concebe-se um sistema socioprodutivo em economia social como estratégia para a autogestão territorial. Palavras-chave: autogestão; construção civil; cultura produtiva; economia social; produção do espaço. Abstract: This paper presents a synthesis of the propositions of the author’s dissertation entitled: “A construção civil na economia social: proposições à cultura produtiva autogestionária.” In this paper, civil construction is problematized as a theoretical and practical location of socioeconomic transformation in order to overcome the capitalist production of space. Thus, one formulates critiques to the productive culture in heterogestionary and autogestionary manners, establishing theoretical and practical propositions to a social economy wide operating in civil construction. These propositions are summarized in the concepts of construction site-school for autogestion and autogestionary construction networks focusing respectively sociopolitical reproduction of the associated work in civil construction and socioeconomic reproduction of associated production in self-managementin territory. Therefore, through the integrated action of work, production and consumption cooperatives in segments such as real estate, infrastructure and building services, it is conceived a socioproductive system in social economy as a strategy for territorial autogestion. Keywords: autogestion; civil construction; productive culture; social economy; production of space.
A nova fábrica é o bairro? O trabalho político e territorial de duas organizações de cooperativas na periferia de Buenos Aires
Authors: Ghibaudi Javier Walter 
Published: January 2017

O artigo discute as mudanças e permanências na ação coletiva de setores dominados na periferia de Buenos Aires na década de 2000, em termos de processos de territorialização e na sua relação com o trabalho. Concentra-se em dois estudos de caso de organizações que se apresentavam como autônomas dos partidos e sindicatos tradicionais, defendiam a criação de relações de trabalhos cooperativas e propunham uma política a partir do bairro. Destaca-se como as suas propostas e ações de trabalho territorial recriam tradições de classe, questionam a divisão entre lutas sociais fora e dentro da fábrica e quebram o senso comum sobre a incapacidade de ação coletiva de pressupostos excluídos sociais. Palavras-chave: cooperativismo; ação coletiva; periferia; Buenos Aires; organizações de trabalhadores desempregados; economia popular e solidária. Abstract: The article discusses the patterns of the collective action and their changes undertaken by the dominated ones in relation to territoriality and labor in the outskirts of Buenos Aires during the first decade of the 21st century. The research focuses on two case studies of organizations that presented themselves as: autonomous from traditional parties and unions, advocated the creation of cooperative work relations and proposed a policy from the “neighborhood”. The article highlights the ways in which these organizations maintain and recreate a working class tradition in contrast with the theories that emphasize the end of social classes and the traditional division, in urban studies, between social struggles outside and inside the factory. Keywords: cooperativism; collective action; periphery; Buenos Aires; unemployed workers; popular and solidarity economy.
Circuitos da economia urbana e economia dos setores populares na fronteira amazônica: o cenário atual no sudeste do Pará
Authors: Silva Harley , Diniz Sibelle , Ferreira Vanessa 
Published: January 2017

Embora a expansão do mercado trabalho formal seja uma tendência sustentada no Brasil nos últimos anos, a persistência de formas de ocupação ligadas ao circuito inferior da economia é uma realidade ampla e mal compreendida. Isso é particularmente verdade para as diversas fronteiras da Amazônia brasileira, onde a economia informal, popular, solidária e familiar persiste, a despeito dos grandes investimentos destinados ao circuito superior. Esse trabalho investiga a situação vigente no sudeste paraense, onde a economia formal de alguns municípios tem crescido a “taxas chinesas” e ao mesmo tempo há a presença massiva de formas alternativas de inserção econômica da população, particularmente a de baixa renda.Palavras-chave: economia dos setores populares; economia informal; circuito inferior; fronteira amazônica; sudeste paraense. Abstract: Although the expansion of formal labor market is a sustained trend in Brazil, occupation in the lower circuit of the economy is persistent and poorly understood. In Brazilian Amazon frontiers, informal, family and solidarity economy persists, in spite of large investments destinated to the upper circuit. This work investigates the current context of the southeast of Pará, where the formal economy of some municipalities has grown at “Chinese rates”, while alternative forms of economic integration incorporate the majority of the population. Keywords: popular economy; informal economy; lower circuit; Amazon frontier; southeast of Pará.
Empresas recuperadas por trabalhadores no Brasil e na Argentina
Authors: Henriques Flávio Chedid , Thiollent Michel Jean-Marie 
Published: January 2017

Este artigo é resultado de uma tese de doutorado que teve como objetivo identificar inovações no campo da organização do trabalho produzidas pelas experiências de empresas recuperadas por trabalhadores no Brasil e na Argentina. A tese central defendida é a de que as limitações impostas pela hegemonia do modo de produção capitalista não encerram a possibilidade de construção de novas relações sociais de produção. Os cinco estudos de caso realizados e a experiência de levantamentos da totalidade das experiências de empresas recuperadas nos dois países forneceram elementos que permitiram problematizar em vários aspectos a organização capitalista do trabalho e, por meio de uma crítica prática, como sugere Rebón (2007), propiciaram a reflexão sobre a possibilidade de superação do modelo hegemônico, que não passa apenas pela inovação no interior das organizações, mas também da relação dessas empresas com seus territórios.Palavras-chave: empresas recuperadas por trabalhadores; organização do trabalho; autogestão; estudos organizacionais críticos. Abstract: This article is the result of a doctoral thesis which aims to identify innovations in the field of labour organization produced by the experiences of companies recovered by workers in Brazil and Argentina. The central thesis defended is that the limitations imposed by the hegemony of the capitalist mode of production do not dismiss the possibility of building new social relations of production. The five case studies and the experience with surveys of all experiences recuperated enterprises in the two countries provided information that allowed questioning in several respects the capitalist organization of work and, through a critical practice, as suggested Rebón (2007), propitiated reflection on the possibility of overcoming the hegemonic model, it is not only about innovation within organizations, but also the relationship of these companies with their territories. Keywords: companies recovered by workers; work organization; workers self-management; critical management studies.