bluuebunny/crossref_metadata_2025_split huggingface dataset:
Streptavidin as a Scaffold for Light‐Induced Long‐Lived Charge Separation
Authors: Keller Sascha G. , Pannwitz Andrea , Mallin Hendrik , Wenger Oliver S. , Ward Thomas R. 
Published: October 2017

AbstractLong‐lived photo‐driven charge separation is demonstrated by assembling a triad on a protein scaffold. For this purpose, a biotinylated triarylamine was added to a RuII–streptavidin conjugate bearing a methyl viologen electron acceptor covalently linked to the N‐terminus of streptavidin. To improve the rate and lifetime of the electron transfer, a negative patch consisting of up to three additional negatively charged amino acids was engineered through mutagenesis close to the biotin‐binding pocket of streptavidin. Time‐resolved laser spectroscopy revealed that the covalent attachment and the negative patch were beneficial for charge separation within the streptavidin hosted triad; the charge separated state was generated within the duration of the excitation laser pulse, and lifetimes up to 3120 ns could be achieved with the optimized supramolecular triad.
Improving Interoperability of Engineering Tools – Data Exchange in Plant Design
Authors: Fillinger Sandra , Bonart Henning , Welscher Wolfgang , Esche Erik , Repke Jens‐Uwe 
Published: October 2017

AbstractSoftware interoperability plays a key role regarding digitalization in process industries. In this contribution, an overview of current developments in the field of data exchange and standardization is given. A prototypical framework for the heterogeneous data exchange between the piping and instrumentation diagram (P&ID) software X‐Visual PlantEngineer, the integrated engineering tool MOSAIC PlantDesign, and the 3D CAD software AVEVA® PDMS is presented for distillation columns. Data exchange between P&ID tools of different vendors, developed by the DEXPI initiative, is considered as an instance of homogeneous data transfer.
Cover Image, Volume 74, Issue 10
Authors: Piasecki Brian P. , Sasani Thomas A. , Lessenger Alexander T. , Huth Nicholas , Farrell Shane 
Published: October 2017

The cover image, by Brian P. Piasecki et al., is based on the Research Article MAPK‐15 is a ciliary protein required for PKD‐2 localization and male mating behavior in Caenorhabditis elegans, DOI: 10.1002/cm.21387.Photo Credit: Alexander T. Lessenger and Brian P. Piasecki.
image
Breast cancer risk associated with atypical hyperplasia and lobular carcinoma in situ initially diagnosed on core‐needle biopsy
Authors: Donaldson Alana R. , McCarthy Caitlin , Goraya Shazia , Pederson Holly J. , Sturgis Charles D. , Grobmyer Stephen R. , Calhoun Benjamin C. 
Published: October 2017

BACKGROUNDBreast cancer risk estimates for atypical lesions are based primarily on case‐control studies of patients with open biopsies. The authors report the cumulative breast cancer incidence after a core biopsy diagnosis of atypical hyperplasia (ductal or lobular) or lobular carcinoma in situ.METHODSA cohort study with central pathology review was conducted on 393 patients who had core biopsy diagnoses of atypical hyperplasia and lobular carcinoma in situ from 1995 through 2010. Follow‐up was available for 255 of 264 patients (97%) at a median of 87 months (range, 3‐236 months).RESULTSThere were 212 patients (54%) who were not upgraded on excision and had no personal history of breast cancer. Of these, 21 of 212 (9.9%) developed breast cancer, including 15 invasive carcinomas, 4 ductal carcinomas in situ, 1 pleomorphic lobular carcinoma in situ, and 1 unknown type. The prior core biopsy diagnoses were atypical ductal hyperplasia for 11 patients (52%) and atypical lobular hyperplasia/lobular carcinoma in situ in the remaining 10 patients (48%). The number of atypical foci in the core biopsy was not significantly associated with the subsequent development of breast cancer (P = .42). Of the 15 invasive carcinomas, 11 (73%) were ipsilateral, 11 (73%) were pathologic T1 tumors, 5 (33%) were pathologic N1 tumors, 13 (87%) were estrogen receptor‐positive, and 1 (7%) was amplified for human epidermal growth factor receptor 2.CONCLUSIONSIn patients who had an initial diagnosis of atypical hyperplasia or lobular carcinoma in situ on core biopsy, the 7‐year cumulative breast cancer incidence was 9.9%. Most tumors were ipsilateral, stage I, estrogen receptor‐positive, invasive carcinomas. The current data support close clinical and radiologic follow‐up for more than 5 years in this patient population. Cancer 2018;124:459‐65. © 2017 American Cancer Society.
Ionic Self‐Assembled Derivative of Tetraphenylethylene: Synthesis, Enhanced Solid‐State Emission, Liquid‐Crystalline Structure, and Cu2+ Detection Ability
Authors: Lu Lin , Ren Xiang‐Kui , Liu Rui , Jiang Xu‐Qiang , Geng Lai‐Yao , Zheng Jun‐Feng , Feng Yakai , Chen Er‐Qiang 
Published: October 2017

AbstractA novel tetraphenylethylene complex composed of 4′,4′′,4′′′,4′′′′‐(ethene‐1,1,2,2‐tetrayl)tetrabiphenyl‐4‐carboxylic acid (H4ETTC) and dimethyldioctadecylammonium bromide (DOAB) with enhanced solid‐state emission is designed and synthesized through an ionic self‐assembly (ISA) strategy. The aggregation‐induced emission property, phase behavior, and supramolecular structure of the complex are characterized by a combination of experimental measurements. The experimental results reveal that the ISA complex can self‐assemble into an ordered helical supramolecular structure with enhanced luminescent properties, although the ETTC cores possess extensive conjugation and high rigidity. Due to the prolonged conjugation length, the fluorescence quantum yield of ETTC–DOAB is boosted to 66 %. Moreover, it is demonstrated that assemblies of the ISA complex are an effective sensor for Cu2+. Owing to the disassembly modulation of ETTC–DOAB aggregations, the fluorescence emission of the assemblies can be selectively and sensitively quenched by Cu2+, with a detection limit as low as 12.6 nm. The enhanced emission efficiency, in combination with the liquid crystallinity and superior sensing performance to Cu2+, make the ETTC–DOAB complex a potential candidate for the fabrication of a luminescent device and chemosensor for Cu2+ detection.
Photocatalytic Synthesis of CdS(core)–CdSe(shell) Quantum Dots with a Heteroepitaxial Junction on TiO2: Photoelectrochemical Hydrogen Generation from Water
Authors: Kitazono Kaoru , Akashi Ryo , Fujiwara Keigo , Akita Atsunobu , Naya Shin‐ichi , Fujishima Musashi , Tada Hiroaki 
Published: October 2017

AbstractThe front cover artwork is provided by the group of Prof. Hiroaki Tada (Kindai University, Japan). The image shows solar‐to‐hydrogen conversion by a photoelectrochemical cell using a CdS(core)–CdSe(shell) hybrid quantum dot with epitaxial junction‐sensitized TiO2 photoanode. Read the full text of the article at 10.1002/cphc.201700708.
Amperometric Detection of dsDNA using an Acridine‐Orange‐Modified Glucose Oxidase
Authors: Jambrec Daliborka , Lammers Klaus , Bobrowski Tim , Pöller Sascha , Schuhmann Wolfgang , Ruff Adrian 
Published: October 2017

AbstractInvited for this month's cover is the group of Prof. Dr. Wolfgang Schuhmann, Dr. Daliborka Jambrec and Dr. Adrian Ruff at Ruhr‐Universität in Bochum, Germany. The cover picture shows a novel procedure for the preferential post‐hybridization labeling of double‐stranded DNA based on the intercalating compound acridine orange, which was covalently bound to glucose oxidase. Labeling with a highly active biocatalyst allows for a simple and sequence‐independent amplification of the signal proportional to the amount of hybridized DNA that may be coupled with other amplification strategies. Read the full text of the article at 10.1002/cplu.201700279.
Mesoporous Copper Nanoparticle Networks Decorated by Graphite Layers for Surface‐Enhanced Raman Scattering Detection of Trace Analytes
Authors: Velegraki Georgia , Xie Jian , Zhang Qichun , Armatas Gerasimos S. 
Published: October 2017

AbstractThe assembly of 3D nanoscale structures of plasmonic nanoparticles (NPs) holds great promise for achieving enhanced optical and electronic properties. This type of materials exhibits a large number of surface hot spots, while offering the possibility for synergetic effects to be observed. Herein, a facile, yet powerful, strategy to fabricate 3D mesoporous networks of copper NPs decorated with graphite layers (denoted as Cu/G) is demonstrated by using a polymer‐assisted self‐assembly method. After thermal processing, the resulting Cu/G‐linked networks retain an open and interconnected porosity with a large surface area (up to 90 m2 g−1) and narrow pore size distribution (ca. 4.3 nm in size). Owing to these characteristics, Cu/G assemblies behave as high‐performance surface‐enhanced Raman scattering (SERS) probes for the detection of analytes in very low concentrations. The substrates comprise low‐cost, environmentally benign materials and show promise for chemical and biological sensing applications.
Ligand Substituents Govern the Efficiency and Mechanistic Path of Hydrogen Production with [Cp*Rh] Catalysts
Authors: Henke Wade C. , Lionetti Davide , Moore William N. G. , Hopkins Julie A. , Day Victor W. , Blakemore James D. 
Published: October 2017

AbstractWe demonstrate that [Cp*Rh] complexes bearing substituted 2,2'‐bipyridyl ligands are effective hydrogen evolution catalysts (Cp*=η5‐pentamethylcyclopentadienyl). Disubstitution (at the 4 and 4' positions) of the bipyridyl ligand (namely −tBu, −H, and −CF3) modulates the catalytic overpotential, in part due to involvement of the reduced ligand character in formally rhodium(I) intermediates. These reduced species are synthesized and isolated here; protonation results in formation of complexes bearing the unusual η4‐pentamethylcyclopentadiene ligand, and the properties of these protonated intermediates further govern the catalytic performance. Electrochemical studies suggest that multiple mechanistic pathways are accessible, and that the operative pathway depends on the applied potential and solution conditions. Taken together, these results suggest synergy in metal–ligand cooperation that modulates the mechanisms of fuel‐forming catalysis with organometallic compounds bearing multiple non‐innocent ligands.
Inflammatory cells in liquid‐based cytology smears classified as bacterial vaginosis
Authors: Eleutério José , Eleutério Renata Mírian Nunes , Martins Leonardo Arruda , Giraldo Paulo César , Gonçalves Ana Katherine Silveira 
Published: October 2017

BackgroundBacterial vaginosis (BV) remains an enigmatic question. The term “osis” instead of “itis” is used because commonly, there are no inflammatory process associated with BV. However, leukocytes are often observed in it.MethodsIn a transversal study, we evaluated 1178 cases with diagnosis of BV by liquid‐based cytology (more than 20% of clue cells), attended in general gynecologic private clinic. Depending of the presence of more than five leukocytes on average per field in immersion objective (1000×), the cases were divided in two groups: few or no leukocytes (< 5 leukocytes per field) (BV) and with leukocytes (≥ 5 leukocytes per field) (BV‐L). The Fisher exact and Student t tests was applied to a confidence interval of 95%. The project was approved by the Ethic Committee of Federal University of Ceará, Brazil.ResultsThe age between the groups was the only different socio‐demographic variable. The assessment of vaginal discharge aspect had no characteristic aspect. Colposcopy findings suggesting colpitis and ectopy were more frequent in the group of BV‐L, 7.1% and 6.9%, respectively. The study of the microbiology demonstrated in the BV‐L group, more frequently co‐occurrence of Candida sp (15.1%) than in BV group (1.5%) (P < .0001). The cellular atypia was present more frequently in the BV‐L (9.6%) than in BV (5.7%) (P = .0116).ConclusionThe presence of leukocytes in BV (or BV‐L) may suggest a mixed infection, ectopy influencing the vaginal milieu and even epithelial atypia.
Loss of BRCA1‐associated protein 1 (BAP1) expression is useful in diagnostic cytopathology of malignant mesothelioma in effusions
Authors: Cozzi Ilaria , Oprescu Florina Anca , Rullo Emma , Ascoli Valeria 
Published: October 2017

BackgroundThe important diagnostic challenge facing the cytopathologist is whether a mesothelial proliferation on effusions represents a malignant mesothelioma (MM) or a benign mesothelial hyperplasia (MH). Here, we evaluated the diagnostic utility of BAP1 immunohistochemistry (IHC) in distinguishing between reactive and neoplastic mesothelial cells.MethodsIn pleural and peritoneal effusions from 147 patients with diagnosed MM or with a differential diagnosis of MM and MH, the expression of BAP1 was examined by IHC on paraffin‐embedded cell blocks (n = 121) and biopsies (n = 44). Included were also synchronous and methacronous cytology/biopsy pair samples. BAP1 IHC was evaluated for nuclear staining as positive or negative on target mesothelial cells, with appropriate internal control.ResultsIn MM cases, loss of BAP1 nuclear staining was observed in 76.5% of the cell blocks and 47.5% of the biopsies. All BAP1‐negative cases with a differential diagnosis of benign and malignant mesothelial proliferations were MM at follow‐up. All MH cases, the 29% of epithelial MM and the 90% of nonepithelial MM, retained BAP1 expression. Synchronous and methacronous biopsy/cytology pairs showed matching BAP1 results.ConclusionIn effusions with mesotheliomatous cells or atypical mesothelial cells of uncertain significance, negative BAP1 IHC strongly supports a diagnosis of MM. With prudence in interpreting immunostaining, BAP1 may be included in IHC panels for MM cytodiagnosis, given its high specificity and sensitivity.
A novel and innovative hair test to determine glucocorticoid levels in racing camels for use in assessment of doping, health, and disease
Authors: Shah Iltaf , Haddow Jody D. , Ibrahim Hiba A. , Sheikh Maryam V. A. , Alhemeiri Fatima S.A. 
Published: October 2017

AbstractThe aim of this project was to develop and validate a new test for the analysis of glucocorticoids in camel hair and to use the new test to analyse hair samples from a variety of camel breeds in sports and racing applications. These findings could be of importance when evaluating racing camels for suspected doping offenses or for injury and disease control. Camel hair samples were collected from 30 non‐racing dromedary camels along with 3 racing camels in Al Ain, UAE and were decontaminated, pulverised, sonicated, and extracted prior to analysis. A liquid chromatographic–mass spectrometric method was employed to determine the levels of glucocorticoids in the hair samples. The 4 drugs of interest, namely hydrocortisone, dexamethasone, flumethasone and methylprednisolone, and an internal standard were quantified in camel hair samples. All 4 of the glucocorticoids were detected in camel hair samples with concentrations ranging between 31 and 935 pg/mg for hydrocortisone, 8–59 pg/mg for dexamethasone, 0.7–1034 pg/mg for flumethasone and 5–66 pg/mg for methylprednisolone in non‐racing camels. One of the racing camels displayed high concentrations of hydrocortisone (1130 pg/mg), flumethasone (2576 pg/mg), methylprednisone (1156 pg/mg) and dexamethasone (29 pg/mg). The authors believe this is the first report of a test for corticosteroids in camel hair. The new test has been validated according to Food and Drug Administration (FDA) guidelines. This new hair test could be useful for further studies in doping control, toxicological studies, pharmacological studies and other clinical applications in camel health, injury, and disease.
Modeling plant composition as community continua in a forest landscape with LiDAR and hyperspectral remote sensing
Authors: Hakkenberg C. R. , Peet R. K. , Urban D. L. , Song C. 
Published: October 2017

AbstractIn light of the need to operationalize the mapping of forest composition at landscape scales, this study uses multi‐scale nested vegetation sampling in conjunction with LiDAR‐hyperspectral remotely sensed data from the G‐LiHT airborne sensor to map vascular plant compositional turnover in a compositionally and structurally complex North Carolina Piedmont forest. Reflecting a shift in emphasis from remotely sensing individual crowns to detecting aggregate optical‐structural properties of forest stands, predictive maps reflect the composition of entire vascular plant communities, inclusive of those species smaller than the resolution of the remotely sensed imagery, intertwined with proximate taxa, or otherwise obscured from optical sensors by dense upper canopies. Stand‐scale vascular plant composition is modeled as community continua: where discrete community‐unit classes at different compositional resolutions provide interpretable context for continuous gradient maps that depict n‐dimensional compositional complexity as a single, consistent RGB color combination. In total, derived remotely sensed predictors explain 71%, 54%, and 48% of the variation in the first three components of vascular plant composition, respectively. Among all remotely sensed environmental gradients, topography derived from LiDAR ground returns, forest structure estimated from LiDAR all returns, and morphological‐biochemical traits determined from hyperspectral imagery each significantly correspond to the three primary axes of floristic composition in the study site. Results confirm the complementarity of LiDAR and hyperspectral sensors for modeling the environmental gradients constraining landscape turnover in vascular plant composition and hold promise for predictive mapping applications spanning local land management to global ecosystem modeling.
Population genomics of the eastern cottonwood (Populus deltoides)
Authors: Fahrenkrog Annette M. , Neves Leandro G. , Resende Márcio F. R. , Dervinis Christopher , Davenport Ruth , Barbazuk W. Brad , Kirst Matias 
Published: October 2017

AbstractDespite its economic importance as a bioenergy crop and key role in riparian ecosystems, little is known about genetic diversity and adaptation of the eastern cottonwood (Populus deltoides). Here, we report the first population genomics study for this species, conducted on a sample of 425 unrelated individuals collected in 13 states of the southeastern United States. The trees were genotyped by targeted resequencing of 18,153 genes and 23,835 intergenic regions, followed by the identification of single nucleotide polymorphisms (SNPs). This natural P. deltoides population showed low levels of subpopulation differentiation (FST = 0.022–0.106), high genetic diversity (θW = 0.00100, π = 0.00170), a large effective population size (Ne ≈ 32,900), and low to moderate levels of linkage disequilibrium. Additionally, genomewide scans for selection (Tajima's D), subpopulation differentiation (XTX), and environmental association analyses with eleven climate variables carried out with two different methods (LFMM and BAYENV2) identified genes putatively involved in local adaptation. Interestingly, many of these genes were also identified as adaptation candidates in another poplar species, Populus trichocarpa, indicating possible convergent evolution. This study constitutes the first assessment of genetic diversity and local adaptation in P. deltoides throughout the southern part of its range, information we expect to be of use to guide management and breeding strategies for this species in future, especially in the face of climate change.
A Behavioral Paradigm to Study Rats Dual‐Task Performance under Head‐Direction and Body‐Location Tracking
Authors: MATSUO KOHEI , KAIJU TARO , NAKAZONO TOMOAKI , WATANABE KEI , SUZUKI TAKAFUMI 
Published: October 2017

SUMMARYDual‐task paradigm is a behavioral procedure in which subjects are required to perform two independent tasks in parallel, each of which involves a distinct stimulus–response association that leads to a unique goal. Although dual tasks are widely used in human studies, they are seldom used in animals. One such rare case was a rat simultaneous temporal processing (STP) task, in which rats were asked to time two different intervals simultaneously . However, there are a few limitations in this paradigm, such that, for example, each of two component tasks was not clearly associated with a unique stimulus–response association. In this report, in order to better characterize temporal dual‐task performance in rats, we developed a modified version of the STP task that comprised two clearly divided component tasks, and equipped our experimental system with a novel video‐based motion tracking system. We show that even under this more rigidly controlled dual‐task setting, rats are able to time two different intervals virtually without interference, and that our tracking system can simultaneously detect head‐direction and body‐location of a rat with high accuracy. These results suggest that the present experimental paradigm should be useful for investigating cognitive processes that underlie dual‐task performance at both the behavioral and neuronal levels.
Development of Automatic Parameter Tuning for Train Automatic Stop Control Device
Authors: ODA ATSUSHI , NIIMI IKUO , MAKI KENTARO 
Published: October 2017

SUMMARYThe adoption of train automatic stop control (TASC) device is expected to increase according to high demand of platform door system. The challenges of TASC are (1) reduction of adjustment man‐hour at TASC introduction and (2) maintenance of stopping position accuracy. These challenges are caused by the difficulty of obtaining vehicle characteristics accurately in real time. To solve the difficulty, a function for automatic parameter tuning for TASC devices has been developed. The function learns vehicle characteristics from real‐time driving data and controls vehicle deceleration based on the learned vehicle characteristics. The developed function was evaluated on a test vehicle, and the results show that it acquired vehicle characteristics accurately and stopped the test vehicle within a stopping accuracy of +/– 35 cm. In addition, it was confirmed that the calculation time was within 70 s. This was less than the target time of 80 s, which is comparable to the average driving time between two stations on a metro line.
The Novel Reconstruction Method for Laser Interferometer with Local Measurement
Authors: KADOWAKI KAZUTAKE , ISHIDA SHUNSUKE , ONO YASUSHI 
Published: October 2017

SUMMARYWe have developed a novel reconstruction method for a laser interferometer to determine the radial electron density profile in a cylindrical plasma vessel under an axial symmetrical assumption with spline interpolation. This method can extend the reconstructible area out of the line‐integrated density measurement area by using the additional local measurement data. Although both the ordinary Abel inversion and the basis function expansion method cannot reconstruct well out of the measurement area, the new method can reconstruct distributions even with nonzero electron density on the wall. In order to confirm the validity of the method, the error sensitivity is evaluated by Monte Carlo simulation.
A Survey and Technology Trends of 3D Features for Object Recognition
Authors: HASHIMOTO MANABU , AKIZUKI SHUICHI , TAKEI SHOICHI 
Published: October 2017

SUMMARYRecently, various kinks of three‐dimensional (3D) imaging devices such as laser range finder or time‐of‐flight sensor become popular, so a lot of practical algorithms for recognizing 3D objects using “point cloud data” have been proposed. In this paper, we will introduce typical object recognition approaches and survey various kinds of 3D features proposed by many researchers. As for some important techniques, their principle and characteristics are explained in detail. Also we will mention about the local reference frame that is very important factor to realize stable feature description and accurate pose estimation in practical use.