bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
Authors: Rout Bishakh , Liu Chi-Hsien , Wu Wei-Chi 
Published: August 2017

AbstractPhotosensitization-based antimicrobial therapy (PAT) is an alternative therapy aimed at achieving bacterial inactivation. Researchers use various photosensitizers to achieve bacterial inactivation. However, the most widely used approach involves the use of photosensitizers dispersed in aqueous solution, which could limit the effectiveness of photodynamic inactivation. Therefore, the approaches to encapsulate the photosensitizer in appropriate vehicles can enhance the delivery of the photosensitizer. Herein, Toluidine Blue O (TBO) was the photosensitizer, and lipid nanoparticles were used for its encapsulation. The lipid nanoparticle-based delivery system has been tailor-made for decreasing the average size and viscosity and increasing the formulation stability as well as the wettability of skin. Usage of an appropriate vehicle will also increase the cellular uptake of the photosensitizer into the bacterial cells, leading to the damage on cell membrane and genomic DNA. Evidence of effectiveness of the developed PAT on planktonic bacteria and biofilms was examined by fluorescence microscopy and scanning electron microscopy. Lipid nanoparticles protected the photosensitizer from aggregation and made the application easy on the skin as indicated in data of size distribution and contact angle. The use of lipid nanoparticles for encapsulating TBO could enhance photosensitization-based antimicrobial therapy as compared to the aqueous media for delivering photosensitizers.
Decoupling Frequencies, Amplitudes and Phases in Nonlinear Optics
Authors: Schmidt Bruno E. , Lassonde Philippe , Ernotte Guilmot , Clerici Matteo , Morandotti Roberto , Ibrahim Heide , Légaré François 
Published: August 2017

AbstractIn linear optics, light fields do not mutually interact in a medium. However, they do mix when their field strength becomes comparable to electron binding energies in the so-called nonlinear optical regime. Such high fields are typically achieved with ultra-short laser pulses containing very broad frequency spectra where their amplitudes and phases are mutually coupled in a convolution process. Here, we describe a regime of nonlinear interactions without mixing of different frequencies. We demonstrate both in theory and experiment how frequency domain nonlinear optics overcomes the shortcomings arising from the convolution in conventional time domain interactions. We generate light fields with previously inaccessible properties by avoiding these uncontrolled couplings. Consequently, arbitrary phase functions are transferred linearly to other frequencies while preserving the general shape of the input spectrum. As a powerful application, we introduce deep UV phase control at 207 nm by using a conventional NIR pulse shaper.
The buckling instability of aggregating red blood cells
Authors: Flormann Daniel , Aouane Othmane , Kaestner Lars , Ruloff Christian , Misbah Chaouqi , Podgorski Thomas , Wagner Christian 
Published: August 2017

AbstractPlasma proteins such as fibrinogen induce the aggregation of red blood cells (RBC) into rouleaux, which are responsible for the pronounced shear thinning behavior of blood, control the erythrocyte sedimentation rate (ESR) – a common hematological test – and are involved in many situations of physiological relevance such as structuration of blood in the microcirculation or clot formation in pathological situations. Confocal microscopy is used to characterize the shape of RBCs within rouleaux at equilibrium as a function of macromolecular concentration, revealing the diversity of contact zone morphology. Three different configurations that have only been partly predicted before are identified, namely parachute, male-female and sigmoid shapes, and quantitatively recovered by numerical simulations. A detailed experimental and theoretical analysis of clusters of two cells shows that the deformation increases nonlinearly with the interaction energy. Models indicate a forward bifurcation in which the contacting membrane undergoes a buckling instability from a flat to a deformed contact zone at a critical value of the interaction energy. These results are not only relevant for the understanding of the morphology and stability of RBC aggregates, but also for a whole class of interacting soft deformable objects such as vesicles, capsules or cells in tissues.
Virtual cardiac monolayers for electrical wave propagation
Authors: Kudryashova Nina , Tsvelaya Valeriya , Agladze Konstantin , Panfilov Alexander 
Published: August 2017

AbstractThe complex structure of cardiac tissue is considered to be one of the main determinants of an arrhythmogenic substrate. This study is aimed at developing the first mathematical model to describe the formation of cardiac tissue, using a joint in silico–in vitro approach. First, we performed experiments under various conditions to carefully characterise the morphology of cardiac tissue in a culture of neonatal rat ventricular cells. We considered two cell types, namely, cardiomyocytes and fibroblasts. Next, we proposed a mathematical model, based on the Glazier-Graner-Hogeweg model, which is widely used in tissue growth studies. The resultant tissue morphology was coupled to the detailed electrophysiological Korhonen-Majumder model for neonatal rat ventricular cardiomyocytes, in order to study wave propagation. The simulated waves had the same anisotropy ratio and wavefront complexity as those in the experiment. Thus, we conclude that our approach allows us to reproduce the morphological and physiological properties of cardiac tissue.
Identifying the tilt angle and correcting the orbital angular momentum spectrum dispersion of misaligned light beam
Authors: Zhao Peng , Li Shikang , Wang Yu , Feng Xue , Kaiyu Cui , Fang Liu , Zhang Wei , Huang Yidong 
Published: August 2017

AbstractThe axis tilt of light beam in optical system would introduce the dispersion of orbital angular momentum (OAM) spectrum. To deal with it, a two-step method is proposed and demonstrated. First, the tilt angle of optical axis is identified with a deduced relation between the tilt angle and the variation of OAM topological charges with different reference axes, which is obtained with the help of a charge coupled device (CCD) camera. In our experiments, the precision of measured tilt angle is about 10−4rad with topological charges of −3~3. With the measured angle value, the additional phase delay due to axis tilt can be calculated so that the dispersion of OAM spectrum can be corrected with a simple formula while the optical axis is not aligned. The experimental results indicate that the original OAM spectrum has been successfully extracted for not only the pure state but also the superposed OAM states.
Palladium nanoparticles entrapped in a self-supporting nanoporous gold wire as sensitive dopamine biosensor
Authors: Yi Xin , Wu Yuxuan , Tan Guoxin , Yu Peng , Zhou Lei , Zhou Zhengnan , Chen Junqi , Wang Zhengao , Pang Jinshan , Ning Chengyun 
Published: August 2017

AbstractTraced dopamine (DA) detection is critical for the early diagnosis and prevention of some diseases such as Parkinson’s, Alzheimer and schizophrenia. In this research, a novel self-supporting three dimensional (3D) bicontinuous nanoporous electrochemical biosensor was developed for the detection of dopamine by Differential Pulse Voltammetry (DPV). This biosensor was fabricated by electrodepositing palladium nanoparticles (Pd) onto self-supporting nanoporous gold (NPG) wire. Because of the synergistic effects of the excellent catalytic activity of Pd and novel structure of NPG wire, the palladium nanoparticles decorated NPG (Pd/NPG) biosensor possess tremendous superiority in the detection of DA. The Pd/NPG wire biosensor exhibited high sensitivity of 1.19 μA μΜ−1, broad detection range of 1–220 μM and low detection limit up to 1 μM. Besides, the proposed dopamine biosensor possessed good stability, reproducibility, reusability and selectivity. The response currents of detection in the fetal bovine serum were also close to the standard solutions. Therefore the Pd/NPG wire biosensor is promising to been used in clinic.
Explosive death induced by mean–field diffusion in identical oscillators
Authors: Verma Umesh Kumar , Sharma Amit , Kamal Neeraj Kumar , Kurths Jürgen , Shrimali Manish Dev 
Published: August 2017

AbstractWe report the occurrence of an explosive death transition for the first time in an ensemble of identical limit cycle and chaotic oscillators coupled via mean–field diffusion. In both systems, the variation of the normalized amplitude with the coupling strength exhibits an abrupt and irreversible transition to death state from an oscillatory state and this first order phase transition to death state is independent of the size of the system. This transition is quite general and has been found in all the coupled systems where in–phase oscillations co–exist with a coupling dependent homogeneous steady state. The backward transition point for this phase transition has been calculated using linear stability analysis which is in complete agreement with the numerics.
Intestinal transepithelial permeability of oxytocin into the blood is dependent on the receptor for advanced glycation end products in mice
Authors: Higashida Haruhiro , Furuhara Kazumi , Yamauchi Agnes-Mikiko , Deguchi Kisaburo , Harashima Ai , Munesue Seiichi , Lopatina Olga , Gerasimenko Maria , Salmina Alla B. , Zhang Jia-Sheng , Kodama Hikari , Kuroda Hironori , Tsuji Chiharu , Suto Satoshi , Yamamoto Hiroshi , Yamamoto Yasuhiko 
Published: August 2017

AbstractPlasma oxytocin (OT) originates from secretion from the pituitary gland into the circulation and from absorption of OT in mother’s milk into the blood via intestinal permeability. However, the molecular mechanism underlying the absorption of OT remains unclear. Here, we report that plasma OT concentrations increased within 10 min after oral delivery in postnatal day 1–7 mice. However, in Receptors for Advanced Glycation End Products (RAGE) knockout mice after postnatal day 3, an identical OT increase was not observed. In adult mice, plasma OT was also increased in a RAGE-dependent manner after oral delivery or direct administration into the intestinal tract. Mass spectrometry evaluated that OT was absorbed intact. RAGE was abundant in the intestinal epithelial cells in both suckling pups and adults. These data highlight that OT is transmitted via a receptor-mediated process with RAGE and suggest that oral OT supplementation may be advantageous in OT drug development.
Relationship between long working hours and periodontitis among the Korean workers
Authors: Lee Wanhyung , Lim Sung-Shil , Kim Byurira , Won Jong-Uk , Roh Jaehoon , Yoon Jin-Ha 
Published: August 2017

AbstractWe aimed to investigate the association between long working hours and periodontitis, and whether such an association constitutes an exposure-response relationship. Data for this study were collected from the Korea National Health and Nutrition Examination Surveys conducted from 2007 to 2014; 17,533 workers (9,483 of men and 8,050 of women) were included. The odds ratios (OR) and 95% confidence intervals (CI) for the analysis of periodontitis defined as positive of Community Periodontal Index in relation to working hours were calculated using multiple logistic regression models with various stratifications. Compared to participants who worked ≤40 hours per week, the prevalence ratio (95% CI) for the periodontitis was 1.19 (1.14–1.24) and full adjusted OR (95% CI) was 1.09 (1.02–1.18) in participants who worked over 40 hours per week. The OR (95% CI) for periodontitis were 1.09 (0.99–1.19) in working group of 40< and ≥52 and 1.10 (1.01–1.20) in working group of >52 hours per week with a significant trend (p = 0.0233) even after adjusting for age, socioeconomic status, healthy behaviour, chronic disease, and dental care status. Long working hours are associated with periodontitis among Korean workers in an exposure-response manner.
Targeting P-glycoprotein: Investigation of piperine analogs for overcoming drug resistance in cancer
Authors: Syed Safiulla Basha , Arya Hemant , Fu I-Hsuan , Yeh Teng-Kuang , Periyasamy Latha , Hsieh Hsing-Pang , Coumar Mohane Selvaraj 
Published: August 2017

AbstractP-glycoprotein (P-gp) is a drug transporter that effluxes chemotherapeutic drugs and is implicated in the development of resistance of cancer cells to chemotherapeutic drugs. To date, no drug has been approved to inhibit P-gp and restore chemotherapy efficacy. Moreover, majority of the reported inhibitors have high molecular weight and complex structures, making it difficult to understand the basic structural requirement for P-gp inhibition. In this study, two structurally simple, low molecular weight piperine analogs Pip1 and Pip2 were designed and found to better interact with P-gp than piperinein silico. A one step, acid-amine coupling reaction between piperic acid and 6,7-dimethoxytetrahydroisoquinoline or 2-(3,4-dimethoxyphenyl)ethylamine afforded Pip1 and Pip2, respectively.In vitrotesting in drug resistant P-gp overexpressing KB (cervical) and SW480 (colon) cancer cells showed that both analogs, when co-administered with vincristine, colchicine or paclitaxel were able to reverse the resistance. Moreover, accumulation of P-gp substrate (rhodamine 123) in the resistant cells, a result of alteration of the P-gp efflux, was also observed. These investigations suggest that the natural product analog – Pip1 ((2E,4E)-5-(benzo[d][1,3]dioxol-5-yl)-1-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1 H)-yl)penta-2,4-dien-1-one) – is superior to piperine and could inhibit P-gp function. Further studies are required to explore the full potential of Pip1 in treating drug resistant cancer.
The role of Snell’s law for a magnonic majority gate
Authors: Kanazawa Naoki , Goto Taichi , Sekiguchi Koji , Granovsky Alexander B. , Ross Caroline A. , Takagi Hiroyuki , Nakamura Yuichi , Uchida Hironaga , Inoue Mitsuteru 
Published: August 2017

AbstractIn the fifty years since the postulation of Moore’s Law, the increasing energy consumption in silicon electronics has motivated research into emerging devices. An attractive research direction is processing information via the phase of spin waves within magnonic-logic circuits, which function without charge transport and the accompanying heat generation. The functional completeness of magnonic logic circuits based on the majority function was recently proved. However, the performance of such logic circuits was rather poor due to the difficulty of controlling spin waves in the input junction of the waveguides. Here, we show how Snell’s law describes the propagation of spin waves in the junction of a Ψ-shaped magnonic majority gate composed of yttrium iron garnet with a partially metallized surface. Based on the analysis, we propose a magnonic counterpart of a core-cladding waveguide to control the wave propagation in the junction. This study has therefore experimentally demonstrated a fundamental building block of a magnonic logic circuit.
Comprehensive miRNA expression profiling in human T-cell acute lymphoblastic leukemia by small RNA-sequencing
Authors: Wallaert Annelynn , Van Loocke Wouter , Hernandez Lucie , Taghon Tom , Speleman Frank , Van Vlierberghe Pieter 
Published: August 2017

AbstractT-cell acute lymphoblastic leukemia (T-ALL) is a genetically heterogeneous disease that can be classified into different molecular genetic subtypes according to their mRNA gene expression profile. In this study, we applied RNA sequencing to investigate the full spectrum of miRNA expression in primary T-ALL patient samples, T-ALL leukemia cell lines and healthy donor thymocytes. Notably, this analysis revealed that genetic subtypes of human T-ALL also display unique miRNA expression signatures, which are largely conserved in human T-ALL cell lines with corresponding genetic background. Furthermore, small RNA-sequencing also unraveled the variety of isoforms that are expressed for each miRNA in T-ALL and showed that a significant number of miRNAs are actually represented by an alternative isomiR. Finally, comparison of CD34+ and CD4+CD8+ healthy donor thymocytes and T-ALL miRNA profiles allowed identifying several novel miRNAs with putative oncogenic or tumor suppressor functions in T-ALL. Altogether, this study provides a comprehensive overview of miRNA expression in normal and malignant T-cells and sets the stage for functional evaluation of novel miRNAs in T-ALL disease biology.
Relationship between higher-order wavefront aberrations and natural progression of myopia in schoolchildren
Authors: Hiraoka Takahiro , Kotsuka Junko , Kakita Tetsuhiko , Okamoto Fumiki , Oshika Tetsuro 
Published: August 2017

AbstractThis study investigated the relationship between higher-order aberrations (HOAs) and myopia progression as well as axial elongation in schoolchildren. We examined cycloplegic refraction, axial length, and wavefront aberrations prospectively in 71 myopic children. Changes in cycloplegic refraction and axial length during a 2-year study period were assessed, and their correlations with HOA components were analyzed. Sixty-four subjects ([mean ± SD] 9.2 ± 1.6 years) completed the 2-year examinations. Cycloplegic refraction was significantly changed after 2 years (P < 0.0001), and the average change (myopia progression) was −1.60 ± 1.04 D. Axial length also increased significantly (P < 0.0001), and the average increase (axial elongation) was 0.77 ± 0.40 mm. Myopia progression and axial elongation showed significant correlations with many components of corneal HOA (P < 0.0001 to P = 0.0270). Multivariate analysis showed that the total HOA of the cornea was the most relevant variable to myopia progression and axial elongation (P < 0.0001). Eyes with larger amounts of corneal HOAs showed less myopia progression and smaller axial elongation, suggesting that corneal HOAs play a role in the refractive and ocular developments in children.
Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
Authors: Karmakar Subhasis , Molla Kutubuddin A. , Das Kaushik , Sarkar Sailendra Nath , Datta Swapan K. , Datta Karabi 
Published: August 2017

AbstractSheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of transgenic rice lines. The first transgenic line overexpresses the rice chitinase gene (OsCHI11); the second contains the Arabidopsis NPR1 (AtNPR1) gene and, the third has pyramided constructs with both the genes (OsCHI11 and AtNPR1). This is a comparative study between the single-gene transgenic lines and the double gene transgenic in terms of their ability to activate the plant defense system. Rice plants of each individual construct were screened via PCR, Southern hybridization, activity assays, and expression analysis. The best transgenic lines of each construct were chosen for comparative study. The fold change in qRT-PCR and activity assays revealed that the pyramided transgenic rice plants show a significant upregulation of defense-related genes, PR genes, and antioxidant marker genes as compared to the single transgene. Simultaneous co-expression of both the genes was found to be more efficient in tolerating oxidative stress. In R. solani (RS) toxin assay, mycelial agar disc bioassay, and in vivo plant bioassay, pyramided transgenic plant lines were more competent at restricting the pathogen development and enhancing sheath blight tolerance as compared to single gene transformants.
Intrusion of the Kuroshio into the South and East China Seas
Authors: Wu Chau-Ron , Wang You-Lin , Lin Yong-Fu , Chao Shenn-Yu 
Published: August 2017

AbstractThe northward-flowing Kuroshio often intrudes westward and modulates the water masses of the South and East China Seas. These intrusions transcend multiple scales in time and space, which we demonstrate here using various independent data sets. There are two hot spots of intrusion, one in the Luzon Strait and the other off northeast Taiwan, which occur synchronously when the upstream Kuroshio weakens during winter. Beyond seasonal time scales, the two intrusions were not synchronous during 1993–2013. While intrusions into the South China Sea echoed the Pacific Decadal Oscillation, the intrusion northeast of Taiwan decreased markedly before 2002 but regularly reached the shelf thereafter. This change was due to the influence of westward impingements of cyclonic eddies from the open ocean on the Kuroshio main stream in place of anticyclonic eddies. During 1993–2001, decreasing cyclonic eddy impingements moved the Kuroshio away from northeast Taiwan, weakening the Kuroshio intrusion onto the East China Sea shelf. Thereafter, enhanced cyclonic eddy impingement during 2002–2013 weakened the Kuroshio transport, moving it closer to the shelf and enhancing its intrusion into the East China Sea.
Repetitive motor cortex stimulation reinforces the pain modulation circuits of peripheral neuropathic pain
Authors: Cha Myeounghoon , Um Sun Woo , Kwon Minjee , Nam Taick Sang , Lee Bae Hwan 
Published: August 2017

AbstractRecent evidence indicates that motor cortex stimulation (MCS) is a potentially effective treatment for chronic neuropathic pain. However, the neural mechanisms underlying the attenuated hyperalgesia after MCS are not completely understood. In this study, we investigated the neural mechanism of the effects of MCS using an animal model of neuropathic pain. After 10 daily sessions of MCS, repetitive MCS reduced mechanical allodynia and contributed to neuronal changes in the anterior cingulate cortex (ACC). Interestingly, inhibition of protein kinase M zeta (PKMζ), a regulator of synaptic plasticity, in the ACC blocked the effects of repetitive MCS. Histological and molecular studies showed a significantly increased level of glial fibrillary acidic protein (GFAP) expression in the ACC after peripheral neuropathy, and neither MCS treatment nor ZIP administration affected this increase. These results suggest that repetitive MCS can attenuate the mechanical allodynia in neuropathic pain, and that the activation of PKMζ in the ACC may play a role in the modulation of neuropathic pain via MCS.