bluuebunny/crossref_metadata_2025_split huggingface dataset:
Training-Intensity Distribution in Road Cyclists: Objective Versus Subjective Measures
Authors: Sanders Dajo , Myers Tony , Akubat Ibrahim 
Published: March 2017

Purpose:To evaluate training-intensity distribution using different intensity measures based on rating of perceived exertion (RPE), heart rate (HR), and power output (PO) in well-trained cyclists.
Methods:Fifteen road cyclists participated in the study. Training data were collected during a 10-wk training period. Training-intensity distribution was quantified using RPE, HR, and PO categorized in a 3-zone training-intensity model. Three zones for HR and PO were based around a 1st and 2nd lactate threshold. The 3 RPE zones were defined using a 10-point scale: zone 1, RPE scores 1–4; zone 2, RPE scores 5–6; zone 3, RPE scores 7–10.
Results:Training-intensity distributions as percentages of time spent in zones 1, 2, and 3 were moderate to very largely different for RPE (44.9%, 29.9%, 25.2%) compared with HR (86.8%, 8.8%, 4.4%) and PO (79.5%, 9.0%, 11.5%). Time in zone 1 quantified using RPE was largely to very largely lower for RPE than PO (P < .001) and HR (P < .001). Time in zones 2 and 3 was moderately to very largely higher when quantified using RPE compared with intensity quantified using HR (P < .001) and PO (P < .001).
Conclusions:Training-intensity distribution quantified using RPE demonstrates moderate to very large differences compared with intensity distributions quantified based on HR and PO. The choice of intensity measure affects intensity distribution and has implications for training-load quantification, training prescription, and the evaluation of training characteristics.
Long-Sprint Abilities in Soccer: Ball Versus Running Drills
Authors: Castagna Carlo , Francini Lorenzo , Póvoas Susana C.A. , D’Ottavio Stefano 
Published: March 2017

Purpose:To examine the acute effects of generic drills (running drills [RDs]) and specific (small-sided-games [SSGs]) long-sprint-ability (LSA) drills on internal and external load of male soccer players.
Methods:Fourteen academy-level soccer players (mean ± SD age 17.6 ± 0.61 y, height 1.81 ± 0.63 m, body mass 69.53 ± 4.65 kg) performed four 30-s LSA bouts for maintenance (work:rest 1:2) and production (1:5) with RDs and SSGs. Players’ external load was tracked with GPS technology (20-Hz), and heart rate (HR), blood lactate concentration (BLc), and rating of perceived exertion (RPE) were used to characterize players’ internal load. Individual peak BLc was assessed with a 30-s all-out test on a nonmotorized treadmill (NMT).
Results:Compared with SSGs, the RDs had a greater effect on external load and BLc (large and small, respectively). During SSGs players covered more distance with high-intensity decelerations (moderate to small). Muscular RPE was higher (small to large) in RDs than in SSGs. The production mode exerted a moderate effect on BLc while the maintenance condition elicited higher cardiovascular effects (small to large).
Conclusion:The results of this study showed the superiority of generic over specific drills in inducing LSA-related physiological responses. In this regard production RDs showed the higher postexercise BLc. Individual peak blood lactate responses were found after the NMT 30-s all-out test, suggesting this drill as a valid option to RDs. The practical physiological diversity among the generic and specific LSA drills here considered enable fitness trainers to modulate prescription of RD and SSG drills for LSA according to training schedule.
The Influence of a Cognitive Dual Task on the Gait Parameters of Healthy Older Adults: A Systematic Review and Meta-Analysis
Authors: Smith Erin , Cusack Tara , Cunningham Caitriona , Blake Catherine 
Published: March 2017

This review examines the effect of a dual task on the gait parameters of older adults with a mean gait speed of 1.0 m/s or greater, and the effect of type and complexity of task. A systematic review of Web of Science, PubMed, SCOPUS, Embase, and PsycINFO was performed in July 2016. Twenty-three studies (28 data sets) were reviewed and pooled for meta-analysis. The effect size on seven gait parameters was measured as the raw mean difference between single- and dual-task performance. Gait speed significantly reduced with the addition of a dual task, with increasing complexity showing greater decrements. Cadence, stride time, and measures of gait variability were all negatively affected under the dual-task condition. In older adults, the addition of a dual task significantly reduces gait speed and cadence, with possible implications for the assessment of older people, as the addition of a dual task may expose deficits not observed under single-task assessment.
Effect of Moderate-Intensity Exercise on Inflammatory Markers Among Postmenopausal Women
Authors: Chagas Eduardo Federighi Baisi , Bonfim Mariana Rotta , Turi Bruna Camilo , Brondino Nair Cristina Margarida , Monteiro Henrique Luiz 
Published: March 2017

Background:Declines in ovarian function in postmenopausal women may contribute to increase inflammatory cytokines, which can lead to chronic diseases. However, studies have shown that exercise interventions are important to manage inflammatory conditions. Thus, the objective of this study was to analyze the effect of exercise intervention on inflammatory markers among obese and postmenopausal women.Methods:70 women composed the sample (Exercise group [EG; n = 35] and nonexercise group [nEG; n = 35]). IL-6, TNF-α, and IL-10 were the inflammatory markers analyzed. Exercise program was 20 weeks long and consisted of aerobic and neuromuscular training. Data about chronic diseases, medication use, dietary intake, body composition and biochemical variables were collected.Results:EG showed significant reductions in body mass index, waist circumference and body fat percentage, as well as increased lean body mass. EG showed significant reductions in TNF-α and significant interaction between group and intervention time. Reductions in IL-10 were identified only in nEG. Substantial effect of exercise intervention was observed with increased ratio of IL-10/IL-6 and IL-10/TNF-α.Conclusions:Combination of aerobic exercise and resistance training was effective in reducing inflammation. Thus, implementation and maintenance of similar exercise programs can contribute to reduce chronic inflammation among obese postmenopausal women.
A Comparative Study of Sleep and Mood Between Young Elite Athletes and Age-Matched Controls
Authors: Harris Anette , Gundersen Hilde , Andreassen Pia Mørk , Thun Eirunn , Bjorvatn Bjørn , Pallesen Ståle 
Published: March 2017

Background:Sleep and mood have seldom been compared between elite athletes and nonelite athletes, although potential differences suggest that physical activity may affect these parameters. This study aims to explore whether adolescent elite athletes differ from controls in terms of sleep, positive affect (PA) and negative affect (NA).Methods:Forty-eight elite athletes and 26 controls participating in organized and nonorganized sport completed a questionnaire, and a 7-day sleep diary.Results:On school days, the athletes and the controls who participated in organized and nonorganized sport differed in bedtime (22:46, 23:14, 23:42, P < .01), sleep onset (23:03, 23:27, 00:12, P < .01), and total sleep time (7:52, 8:00, 6:50, P < 01). During weekend, the athletes, the controls who participated in organized and nonorganized sport differed in bedtime (23:30, 00:04, 00:49, P < .01), sleep onset (23.42, 00:18, 01:13, P < .01), rise time (9:15, 9:47, 10:55, P < .01), sleep efficiency (95.0%, 94.2%, 90.0%, P < 05), and sleep onset latency (11.8, 18.0, 28.0 minutes, P < .01). Furthermore, the athletes reported less social jetlag (0:53) and higher score for PA (34.3) compared with the controls who participated in nonorganized sport (jetlag: 1:25, P < .05, PA: 29.8, P < .05).Conclusions:An almost dose-response association was found between weekly training hours, sleep, social jetlag and mood in adolescents.
Tuberculous Lymphadenitis Is Associated with Enhanced Baseline and Antigen-Specific Induction of Type 1 and Type 17 Cytokines and Reduced Interleukin-1β (IL-1β) and IL-18 at the Site of Infection
Authors: Kathamuthu Gokul Raj , Moideen Kadar , Baskaran Dhanaraj , Banurekha Vaithilingam V. , Nair Dina , Sekar Gomathi , Sridhar Rathinam , Vidyajayanthi Bharathi , Gajendraraj Ganeshan , Parandhaman Dinesh Kumar , Srinivasan Alena , Babu Subash 
Published: March 2017

ABSTRACT

Tuberculous lymphadenitis (TBL) is characterized by an expansion of Th1 and Th17 cells with altered serum levels of proinflammatory cytokines. However, the cytokine profile at the site of infection, i.e., the affected lymph nodes, has not been examined in detail. To estimate the baseline and mycobacterial antigen-stimulated concentrations of type 1, type 17, and other proinflammatory cytokines in patients with TBL (
n
= 14), we examined both the baseline and the antigen-specific concentrations of these cytokines before and after chemotherapy and compared them with those in individuals with pulmonary tuberculosis (PTB) (
n
= 14). In addition, we also compared the cytokine responses in whole blood and those in the lymph nodes of TBL individuals. We observed significantly enhanced baseline and antigen-specific levels of type 1 cytokines (gamma interferon [IFN-γ] and tumor necrosis factor alpha [TNF-α]) and a type 17 cytokine (interleukin-17 [IL-17]) and significantly diminished baseline and antigen-specific levels of proinflammatory cytokines (IL-1β and IL-18) in the whole blood of TBL individuals compared to those in the whole blood of PTB individuals. Moreover, we also observed a pattern of baseline and antigen-specific cytokine production at the site of infection (lymph node) similar to that in the whole blood of TBL individuals. Following standard antituberculosis (anti-TB) treatment, we observed alterations in the baseline and/or antigen-specific levels of IFN-γ, TNF-α, IL-1β, and IL-18. TBL is therefore characterized by enhanced baseline and antigen-specific production of type 1 and type 17 cytokines and reduced baseline and antigen-specific production of IL-1β and IL-18 at the site of infection.
Whole-Genome Sequencing in Epidemiology of Campylobacter jejuni Infections
Authors: Llarena Ann-Katrin , Taboada Eduardo , Rossi Mirko 
Published: March 2017

ABSTRACT

This review describes the current state of knowledge regarding the application of whole-genome sequencing (WGS) in the epidemiology of
Campylobacter jejuni
, the leading cause of bacterial gastroenteritis worldwide. We describe how WGS has increased our understanding of the evolutionary and epidemiological dynamics of this pathogen and how WGS has the potential to improve surveillance and outbreak detection. We have identified hurdles to the full implementation of WGS in public health settings. Despite these challenges, we think that ample evidence is available to support the benefits of integrating WGS into the routine monitoring of
C. jejuni
infections and outbreak investigations.
Assessment of Rapid-Blood-Culture-Identification Result Interpretation and Antibiotic Prescribing Practices
Authors: Donner Linsey M. , Campbell W. Scott , Lyden Elizabeth , Van Schooneveld Trevor C. 
Published: March 2017

ABSTRACT

Rapid pathogen identification can alter antibiotic prescribing practices if interpreted correctly. Microbiology reporting can be difficult to understand, and new technology has made it more challenging. Nebraska Medicine recently implemented the BioFire FilmArray blood culture identification panel (BCID) coupled with stewardship-based education on interpretation. Physician BCID result interpretation and prescribing were assessed via an electronic survey, with a response rate of 40.8% (156/382 surveys). Seven questions required respondents to interpret BCID results, identify the most likely pathogen, and then choose therapy based on the results. The tallied correct responses resulted in a knowledge score. General linear models evaluated the effect of role, specialty, and utilization of the BCID interpretation guide on the mean knowledge score. The specialties of the respondents included 55.7% internal medicine, 19.7% family medicine, and 24.6% other. Roles included 41.1% residents, 5.0% fellows, and 53.9% faculty. Most reported that they reviewed antimicrobial susceptibility results (89.4%) and adjusted therapy accordingly (81.6%), while only 60% stated that they adjusted therapy based on BCID results. The correct response rates ranged from 52 to 86% for the interpretation questions. The most common errors included misinterpretation of
Enterobacteriaceae
and
Staphylococcus
genus results. Neither role nor specialty was associated with total knowledge score in multivariate analysis (
P
= 0.13 and 0.47, respectively). In conclusion, physician interpretation of BCID results is suboptimal and can result in ineffective treatment or missed opportunity to narrow therapy. With the implementation of new technology, improved reporting practices of BCID results with clinical decision support tools providing interpretation guidance available at the point of care is recommended.
Imaging HIV-1 Genomic DNA from Entry through Productive Infection
Authors: Stultz Ryan D. , Cenker Jennifer J. , McDonald David 
Published: March 2017

ABSTRACT

In order to track the fate of HIV-1 particles from early entry events through productive infection, we developed a method to visualize HIV-1 DNA reverse transcription complexes by the incorporation and fluorescent labeling of the thymidine analog 5-ethynyl-2′-deoxyuridine (EdU) into nascent viral DNA during cellular entry. Monocyte-derived macrophages were chosen as natural targets of HIV-1, as they do not divide and therefore do not incorporate EdU into chromosomal DNA, which would obscure the detection of intranuclear HIV-1 genomes. Using this approach, we observed distinct EdU puncta in the cytoplasm of infected cells within 12 h postinfection and subsequent accumulation of puncta in the nucleus, which remained stable through 5 days. The depletion of the restriction factor SAMHD1 resulted in a markedly increased number of EdU puncta, allowing efficient quantification of HIV-1 reverse transcription events. Analysis of HIV-1 isolates bearing defined mutations in the capsid protein revealed differences in their cytoplasmic and nuclear accumulation, and data from quantitative PCR analysis closely recapitulated the EdU results. RNA fluorescence
in situ
hybridization identified actively transcribing, EdU-labeled HIV-1 genomes in productively infected cells, and immunofluorescence analysis confirmed that CDK9, phosphorylated at serine 175, was recruited to RNA-positive HIV-1 DNA, providing a means to directly observe transcriptionally active HIV-1 genomes in productively infected cells. Overall, this system allows stable labeling and monitoring of HIV genomic DNA within infected cells during cytoplasmic transit, nuclear import, and mRNA synthesis.

IMPORTANCE
The fates of HIV-1 reverse transcription products within infected cells are not well understood. Although previous imaging approaches identified HIV-1 intermediates during early stages of infection, few have connected these events with the later stages that ultimately lead to proviral transcription and the production of progeny virus. Here we developed a technique to label HIV-1 genomes using modified nucleosides, allowing subsequent imaging of cytoplasmic and nuclear HIV-1 DNA in infected monocyte-derived macrophages. We used this technique to track the efficiency of nuclear entry as well as the fates of HIV-1 genomes in productively and nonproductively infected macrophages. We visualized transcriptionally active HIV-1 DNA, revealing that transcription occurs in a subset of HIV-1 genomes in productively infected cells. Collectively, this approach provides new insights into the nature of transcribing HIV-1 genomes and allows us to track the entire course of infection in macrophages, a key target of HIV-1 in infected individuals.
Functional Evidence of the Involvement of the Dynein Light Chain DYNLRB2 in Murine Leukemia Virus Infection
Authors: Opazo Tatiana , Garcés Andrea , Tapia Diego , Barraza Felipe , Bravo Angélica , Schwenke Tomás , Cancino Jorge , Arriagada Gloria 
Published: March 2017

ABSTRACT
How murine leukemia virus (MLV) travels from the cell membrane to the nucleus and the mechanism for nuclear entry of MLV DNA in dividing cells still remain unclear. It seems likely that the MLV preintegration complex (PIC) interacts with cellular proteins to perform these tasks. We recently published that the microtubule motor cytoplasmic dynein complex and its regulator proteins interact with the MLV PIC at early times of infection, suggesting a functional interaction between the incoming viral particles, the dynein complex, and dynein regulators. To better understand the role of the dynein complex in MLV infection, we performed short hairpin RNA (shRNA) screening of the dynein light chains on MLV infection. We found that silencing of a specific light chain of the cytoplasmic dynein complex, DYNLRB2, reduced the efficiency of infection by MLV reporter viruses without affecting HIV-1 infection. Furthermore, the overexpression of DYNLRB2 increased infection by MLV. We conclude that the DYNLRB2 light chain of the cytoplasmic dynein complex is an important and specific piece of the host machinery needed for MLV infection.

IMPORTANCE
Retroviruses must reach the chromatin of their host to integrate their viral DNA, but first they must get into the nucleus. The cytoplasm is a crowded environment in which simple diffusion is slow, and thus viruses utilize retrograde transport along the microtubule network, mediated by the dynein complex. Different viruses use different components of this multisubunit complex. We have found that murine leukemia virus (MLV) associates functionally and specifically with the dynein light chain DYNLRB2, which is required for infection. Our study provides more insight into the molecular requirements for retrograde transport of the MLV preintegration complex and demonstrates, for the first time, a role for DYNLRB2 in viral infection.
New Connections: Cell-to-Cell HIV-1 Transmission, Resistance to Broadly Neutralizing Antibodies, and an Envelope Sorting Motif
Authors: Smith S. Abigail , Derdeyn Cynthia A. 
Published: March 2017

ABSTRACT

HIV-1 infection from cell-to-cell may provide an efficient mode of viral spread
in vivo
and could therefore present a significant challenge for preventative or therapeutic strategies based on broadly neutralizing antibodies. Indeed, Li et al. (H. Li, C. Zony, P. Chen, and B. K. Chen, J. Virol. 91:e02425-16, 2017,
https://doi.org/10.1128/JVI.02425-16
) showed that the potency and magnitude of multiple HIV-1 broadly neutralizing antibody classes are decreased during cell-to-cell infection in a context-dependent manner. A functional motif in gp41 appears to contribute to this differential susceptibility by modulating exposure of neutralization epitopes.
Conformational States of a Soluble, Uncleaved HIV-1 Envelope Trimer
Authors: Liu Yuhang , Pan Junhua , Cai Yongfei , Grigorieff Nikolaus , Harrison Stephen C. , Chen Bing 
Published: March 2017

ABSTRACT

The HIV-1 envelope spike [Env; trimeric (gp160)
3
cleaved to (gp120/gp41)
3
] induces membrane fusion, leading to viral entry. It is also the viral component targeted by neutralizing antibodies. Vaccine development requires production, in quantities suitable for clinical studies, of a recombinant form that resembles functional Env. HIV-1 gp140 trimers—the uncleaved ectodomains of (gp160)
3
—from a few selected viral isolates adopt a compact conformation with many antigenic properties of native Env spikes. One is currently being evaluated in a clinical trial. We report here low-resolution (20 Å) electron cryomicroscopy (cryoEM) structures of this gp140 trimer, which adopts two principal conformations, one closed and the other slightly open. The former is indistinguishable at this resolution from those adopted by a stabilized, cleaved trimer (SOSIP) or by a membrane-bound Env trimer with a truncated cytoplasmic tail (EnvΔCT). The latter conformation is closer to a partially open Env trimer than to the fully open conformation induced by CD4. These results show that a stable, uncleaved HIV-1 gp140 trimer has a compact structure close to that of native Env.

IMPORTANCE
Development of any HIV vaccine with a protein component (for either priming or boosting) requires production of a recombinant form to mimic the trimeric, functional HIV-1 envelope spike in quantities suitable for clinical studies. Our understanding of the envelope structure has depended in part on a cleaved, soluble trimer, known as SOSIP.664, stabilized by several modifications, including an engineered disulfide. This construct, which is difficult to produce in large quantities, has yet to induce better antibody responses than those to other envelope-based immunogens, even in animal models. The uncleaved ectodomain of the envelope protein, called gp140, has also been made as a soluble form to mimic the native Env present on the virion surface. Most HIV-1 gp140 preparations are not stable, however, and have an inhomogeneous conformation. The results presented here show that gp140 preparations from suitable isolates can adopt a compact, native-like structure, supporting its use as a vaccine candidate.
Cytoplasmic Motifs in the Nipah Virus Fusion Protein Modulate Virus Particle Assembly and Egress
Authors: Johnston Gunner P. , Contreras Erik M. , Dabundo Jeffrey , Henderson Bryce A. , Matz Keesha M. , Ortega Victoria , Ramirez Alfredo , Park Arnold , Aguilar Hector C. 
Published: March 2017

ABSTRACT

Nipah virus (NiV), a paramyxovirus in the genus
Henipavirus
, has a mortality rate in humans of approximately 75%. While several studies have begun our understanding of NiV particle formation, the mechanism of this process remains to be fully elucidated. For many paramyxoviruses, M proteins drive viral assembly and egress; however, some paramyxoviral glycoproteins have been reported as important or essential in budding. For NiV the matrix protein (M), the fusion glycoprotein (F) and, to a much lesser extent, the attachment glycoprotein (G) autonomously induce the formation of virus-like particles (VLPs). However, functional interactions between these proteins during assembly and egress remain to be fully understood. Moreover, if the F-driven formation of VLPs occurs through interactions with host cell machinery, the cytoplasmic tail (CT) of F is a likely interactive domain. Therefore, we analyzed NiV F CT deletion and alanine mutants and report that several but not all regions of the F CT are necessary for efficient VLP formation. Two of these regions contain YXXØ or dityrosine motifs previously shown to interact with cellular machinery involved in F endocytosis and transport. Importantly, our results showed that F-driven, M-driven, and M/F-driven viral particle formation enhanced the recruitment of G into VLPs. By identifying key motifs, specific residues, and functional viral protein interactions important for VLP formation, we improve our understanding of the viral assembly/egress process and point to potential interactions with host cell machinery.

IMPORTANCE
Henipaviruses can cause deadly infections of medical, veterinary, and agricultural importance. With recent discoveries of new henipa-like viruses, understanding the mechanisms by which these viruses reproduce is paramount. We have focused this study on identifying the functional interactions of three Nipah virus proteins during viral assembly and particularly on the role of one of these proteins, the fusion glycoprotein, in the incorporation of other viral proteins into viral particles. By identifying several regions in the fusion glycoprotein that drive viral assembly, we further our understanding of how these viruses assemble and egress from infected cells. The results presented will likely be useful toward designing treatments targeting this aspect of the viral life cycle and for the production of new viral particle-based vaccines.