bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD20‐Specific Immunoligands Engaging NKG2D Enhance γδ T Cell‐Mediated Lysis of Lymphoma Cells
Authors: Peipp M. , Wesch D. , Oberg H.‐H. , Lutz S. , Muskulus A. , van de Winkel J. G. J. , Parren P. W. H. I. , Burger R. , Humpe A. , Kabelitz D. , Gramatzki M. , Kellner C. 
Published: July 2017

AbstractHuman γδ T cells are innate‐like T cells which are able to kill a broad range of tumour cells and thus may have potential for cancer immunotherapy. The activating receptor natural killer group 2 member D (NKG2D) plays a key role in regulating immune responses driven by γδ T cells. Here, we explored whether recombinant immunoligands consisting of a CD20 single‐chain fragment variable (scFv) linked to a NKG2D ligand, either MHC class I chain‐related protein A (MICA) or UL16 binding protein 2 (ULBP2), could be employed to engage γδ T cells for tumour cell killing. The two immunoligands, designated MICA:7D8 and ULBP2:7D8, respectively, enhanced cytotoxicity of ex vivo‐expanded γδ T cells against CD20‐positive lymphoma cells. Both Vδ1 and Vδ2 γδ T cells were triggered by MICA:7D8 or ULBP2:7D8. Killing of CD20‐negative tumour cells was not induced by the immunoligands, indicating their antigen specificity. MICA:7D8 and ULBP2:7D8 acted in a dose‐dependent manner and induced cytotoxicity at nanomolar concentrations. Importantly, chronic lymphocytic leukaemia (CLL) cells isolated from patients were sensitized by the two immunoligands for γδ T cell cytotoxicity. In a combination approach, the immunoligands were combined with bromohydrin pyrophosphate (BrHPP), an agonist for Vδ2 γδ T cells, which further enhanced the efficacy in target cell killing. Thus, employing tumour‐directed recombinant immunoligands which engage NKG2D may represent an attractive strategy to enhance antitumour cytotoxicity of γδ T cells.
Thermomechanical characterization of stress localization in glass: An experimental and numerical study
Authors: Corvec Guillaume , Robin Éric , Le Cam Jean‐Benoît , Lucas Pierre , Sangleboeuf Jean‐Christophe , Canevet Frédéric 
Published: July 2017

AbstractThermoelastic stress analysis and quantitative calorimetry are full‐field noncontact techniques widely used to study the thermomechanical behaviour of materials. The first one linearly relates the sum of the principal stresses to the temperature variation, and the second one can be used to measure the mechanical dissipation. However, brittle materials such as glass are a priori bad candidates for these techniques. Indeed, their low‐temperature variations under loading lead to very noisy infrared images, and their brittle mechanical behaviour does not allow to deform them significantly. In the present paper, the thermomechanical characterization of a holed glass sample under cyclic loading is performed. A preliminary new filtering methodology has been applied to the thermal movie to remove the noise. The stress field obtained from the thermoelastic stress analysis is well correlated to the finite element model showing that this technique is adapted to study the thermoelastic response of brittle materials. Finally, the corresponding calorimetric response has been determined by using a simplified formulation of the heat diffusion equation. This permits to quantify heat sources and to carry out energy balances.
Complete Genome Sequence of Streptococcus pneumoniae Virulent Phage MS1
Authors: Kot Witold , Sabri Mourad , Gingras Hélène , Ouellette Marc , Tremblay Denise M. , Moineau Sylvain 
Published: July 2017

ABSTRACT

The lytic
Streptococcus pneumoniae
phage MS1 was isolated from a throat swab of a patient with symptoms of upper respiratory tract infection. The genome of this siphophage has 56,075 bp, 42.3% G+C content, and 77 open reading frames, including queuosine biosynthesis genes. Phage MS1 is related to pneumococcal phage Dp-1.
Whole-Genome Sequences of Influenza A(H1N1)pdm09 Virus Isolates from Kerala, India
Authors: Jones Sara , Prasad Raji , Nair Anjana S. , Dharmaseelan Sanjai , Usha Remya , Nair Radhakrishnan R. , Pillai Radhakrishna Madhavan 
Published: July 2017

ABSTRACT
We report here the whole-genome sequence of six clinical isolates of influenza A(H1N1)pdm09, isolated from Kerala, India. Amino acid analysis of all gene segments from the A(H1N1)pdm09 isolates obtained in 2014 and 2015 identified several new mutations compared to the 2009 A(H1N1) pandemic strain.
Draft Genome Sequence of Bacillus altitudinis YNP4-TSU, Isolated from Yellowstone National Park
Authors: OHair Joshua A. , Li Hui , Thapa Santosh , Scholz Matthew , Zhou Suping 
Published: July 2017

ABSTRACT

Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulolytic thermophiles. We report here the draft genome sequence of one of these isolates,
Bacillus altitudinis
YNP4-TSU.
Which foods are displaced in the diets of adults with type 2 diabetes with the inclusion of eggs in their diets? A randomized, controlled, crossover trial
Authors: Njike Valentine Y , Annam Rachel , Costales Victoria Christina , Yarandi Niloufarsadat , Katz David L 
Published: July 2017

BackgroundThe inclusion or exclusion of specific foods from the overall diet inevitably affects other food choices, and this matter is routinely neglected in dietary guidance and nutritional epidemiology. We examined how the inclusion of eggs in the diets of type 2 diabetics affected dietary pattern.MethodsRandomized, controlled, single-blind, crossover trial of 34 adults (mean age 64.5 years; 14 women, 20 men) with type 2 diabetes assigned to one of two possible sequence permutations of two different 12-week treatments (two eggs/day or egg exclusion), with 6-week washout periods. For the egg inclusion phase, participants received advice from a dietitian on how to preserve an isocaloric condition relative to the egg exclusion phase. To assess changes in dietary pattern in the diets of our study participants, we analyzed the 12 components of the 2010 Healthy Eating Index.ResultsThe inclusion of eggs was associated with reduced consumption of refined grains nearing statistical significance (−0.7±3.4 vs 0.7±2.2; p=0.0530). The consumption of total protein foods significantly increased from baseline (0.3±0.7; p=0.0153) with the inclusion of eggs for 12 weeks, while the consumption of dairy products significantly decreased with the exclusion of eggs from their diets (−1.3±2.9; p=0.0188).ConclusionsEggs in the diets of type 2 diabetics may lead to increased consumption of some healthful foods and reduced consumption of some less healthful foods.Trial registration numberNCT02052037; Post-results.
Development of an intervention to support patients and clinicians with advanced lung cancer when considering systemic anticancer therapy: protocol for the PACT study
Authors: Anagnostou Despina , Sivell Stephanie , Noble Simon , Lester Jason , Byrne Anthony , Sampson Catherine , Longo Mirella , Nelson Annmarie 
Published: July 2017

IntroductionPatient-centred care is essential to the delivery of healthcare; however, this necessitates direct patient involvement in clinical decision-making and can be challenging for patients diagnosed with advanced non-small cell lung cancer where there may be misunderstanding of the extent of disease, prognosis and aims of treatment. In this context, decisions are complex and there is a need to balance the risks and benefits, including treatment with palliative intent. The aim of the PACT study is to identify the information and decision support needs of patients, leading to the development of an intervention to support patients with advanced lung cancer when considering treatment options.Methods and analysisPACT is a five-stage, multimethod and multicentre study.Participants: Patients and health professionals will be recruited from three health boards.Methods: Non-participant observation of multidisciplinary team meetings (n=12) will be used to determine patients’ allocation to treatment pathways (stage I). Non-participant observation of patient–clinician consultations (n=20–30) will be used to explore communication of treatment options and decision-making. Extent of participation in decision-making will be assessed using the Observing Patient Involvement in Shared Decision-Making tool. Interviews with patients (stage III) and their clinicians (stage IV) will explore the perception of treatment options and involvement in decision-making. Based on stages I–IV, an expert consensus meeting will finalise the content and format of the intervention. Cognitive interviews with patients will then determine the face validity of the intervention (stage V).Analysis: analysis will be according to data type and research question and will include mediated discourse analysis, thematic analysis, framework analysis and interpretative phenomenological analysis.Ethics and disseminationEthical approval has been granted. The study findings will contribute to and promote shared and informed decision-making in the best interest of patients and prudent healthcare. We therefore aim to disseminate results via relevant respiratory, oncology and palliative care journals and conferences.
A method to assess obstetric outcomes using the 10-Group Classification System: a quantitative descriptive study
Authors: Rossen Janne , Lucovnik Miha , Eggebø Torbjørn Moe , Tul Natasa , Murphy Martina , Vistad Ingvild , Robson Michael 
Published: July 2017

ObjectivesInternationally, the 10-Group Classification System (TGCS) has been used to report caesarean section rates, but analysis of other outcomes is also recommended. We now aim to present the TGCS as a method to assess outcomes of labour and delivery using routine collection of perinatal information.DesignThis research is a methodological study to describe the use of the TGCS.SettingStavanger University Hospital (SUH), Norway, National Maternity Hospital Dublin, Ireland and Slovenian National Perinatal Database (SLO), Slovenia.Participants9848 women from SUH, Norway, 9250 women from National Maternity Hospital Dublin, Ireland and 106 167 women, from SLO, Slovenia.Main outcome measuresAll women were classified according to the TGCS within which caesarean section, oxytocin augmentation, epidural analgesia, operative vaginal deliveries, episiotomy, sphincter rupture, postpartum haemorrhage, blood transfusion, maternal age >35 years, body mass index >30, Apgar score, umbilical cord pH, hypoxic–ischaemic encephalopathy, antepartum and perinatal deaths were incorporated.ResultsThere were significant differences in the sizes of the groups of women and the incidences of events and outcomes within the TGCS between the three perinatal databases.ConclusionsThe TGCS is a standardised objective classification system where events and outcomes of labour and delivery can be incorporated. Obstetric core events and outcomes should be agreed and defined to set standards of care. This method provides continuous and available observations from delivery wards, possibly used for further interpretation, questions and international comparisons. The definition of quality may vary in different units and can only be ascertained when all the necessary information is available and considered together.
Bone mineral density in patients with psoriatic arthritis: data from the Nord-Trøndelag Health Study 3
Authors: Gulati Agnete Malm , Hoff Mari , Salvesen Øyvind , Dhainaut Alvilde , Semb Anne Grete , Kavanaugh Arthur , Haugeberg Glenn 
Published: July 2017

BackgroundThe risk of osteoporosis in patients with psoriatic arthritis (PsA) remains unclear. The aim of this study was to compare bone mineral density (BMD) measured by dual-energy X-ray absorptiometry (DXA) in patients with PsA and controls.Patients and methodsPatients with PsA and controls were recruited from the Nord-Trøndelag Health Study (HUNT) 3.ResultsPatients with PsA (n=69) and controls (n=11 703) were comparable in terms of age (56.8 vs 55.3 years, p=0.32), gender distribution (females 65.2% vs 64.3%, p=0.87) and postmenopausal status (75.6% vs 62.8%, p=0.08). Body mass index (BMI) was higher in patients with PsA compared with controls (28.5 vs 27.2 kg/m2, p=0.01). After adjusting for potential confounding factors (including BMI), BMD was higher in patients with PsA compared with controls at lumbar spine 1–4 (1.213 vs 1.147 g/cm2, p=0.003) and femoral neck (0.960 vs 0.926 g/cm2, p=0.02), but not at total hip (1.013 vs 0.982 g/cm2, p=0.11). Controls had significantly higher odds of having osteopenia or osteoporosis based on measurements of BMD in both the femoral neck (p=0.001), total hip (p=0.033) and lumbar spine (p=0.033).ConclusionOur population-based data showed comparable BMD in patients with PsA and controls. This supports that the PsA population is not at increased risk of osteoporosis.
Effects of strict prolonged bed rest on cardiorespiratory fitness: systematic review and meta-analysis
Authors: Ried-Larsen Mathias , Aarts Hugo M. , Joyner Michael J. 
Published: July 2017

The aim of this systematic review and meta-analysis [International Prospective Register of Systematic Reviews (PROSPERO) CRD42017055619] was to assess the effects of strict prolonged bed rest (without countermeasures) on maximal oxygen uptake (V̇o2max) and to explore sources of variation therein. Since 1949, 80 studies with a total of 949 participants (>90% men) have been published with data on strict bed rest and V̇o2max. The studies were conducted mainly in young participants [median age (interquartile range) 24.5 (22.4–34.0) yr]. The duration of bed rest ranged from 1 to 90 days. V̇o2max declined linearly across bed rest duration. No statistical difference in the decline among studies reporting V̇o2max as l/min (−0.3% per day) compared with studies reporting V̇o2max normalized to body weight (ml·kg−1·min−1; −0.43% per day) was observed. Although both total body weight and lean body mass declined in response to bed rest, we did not see any associations with the decline in V̇o2max. However, 15–26% of the variation in the decline in V̇o2max was explained by the pre-bed-rest V̇o2max levels, independent of the duration of bed rest (i.e., higher pre-bed-rest V̇o2max levels were associated with larger declines in V̇o2max). Furthermore, the systematic review revealed a gap in the knowledge about the cardiovascular response to extreme physical inactivity, particularly in older subjects and women of any age group. In addition to its relevance to spaceflight, this lack of data has significant translational implications because younger women sometimes undergo prolonged periods of bed rest associated with the complications of pregnancy and the incidence of hospitalization including prolonged periods of bed rest increases with age. NEW & NOTEWORTHY Large interindividual responses of maximal oxygen uptake (V̇o2max) to aerobic exercise training exist. However, less is known about the variability in the response of V̇o2max to prolonged bed rest. This systematic review and meta-analysis showed that pre-bed-rest V̇o2max values were inversely associated with the change in V̇o2max independent of the duration of bed rest. Moreover, we identified a large knowledge gap about the causes of decline in V̇o2max, particularly in postmenopausal women, which may have clinical implications.
ПОРУШЕННЯ ЛІПІДНОГО ПРОФІЛЮ У ХВОРИХ З ХРОНІЧНИМ ПАНКРЕАТИТОМ
Authors: Opalenyk S. M. , Sirchak E. S. 
Published: July 2017

У статті описано та проведено аналіз дисліпідемічних порушень у хворих на хронічний панкреатит.Мета дослідження – виявити та дослідити зміни ліпідного спектра крові при хронічному панкреатиті (ХП) в залежності від зовнішньосекреторної недостатності підшлункової залози.Матеріал і методи. Нами було проведено 13С–змішаний тригліцеридний дихальний тест (13С–ЗТДТ) та 13С–амілазний дихальний тест (13С–АДТ) для виявлення зовнішньосекреторної недостатності підшлункової залози (ЗСН ПЗ). Зміни ліпідного профілю крові ми визначали за наявністю порушень у ліпідограмі (визначенням вмісту загального холестерину плазми крові (ЗХ), тригліцеридів (ТГ), холестерину ліпопротеїдів високої щільності (ЛПВЩ) і ліпопротеїдів низької щільності (ЛПНЩ), коефіцієнта атерогенності (КА)).Результати. У майже половини пацієнтів виявлено порушення ліпідного спектра крові у вигляді зростання ЛПНЩ, ЛПДНЩ, ЗХ, ТГ, КА та зниження рівня ЛПВЩ. Доведена залежність між зростанням ЗСН ПЗ та дисліпідемічними порушеннями у хворих на ХП, а саме – тяжчий перебіг захворювання з вираженішою ЗСН ПЗ та, відповідно, гіршим прогнозом. Встановлено, що перебіг ХП із супутніми атеросклеротичними змінами супроводжується зниженням сумарної концентрації 13СО2 в кінці дослідження до (16,3±0,7) % (р<0,05) при проведенні 13С–ЗТДТ та (6,9±1,2) % (р<0,05) при проведенні 13С–АДТ відповідно.
STRUCTURAL CONDITION OF PANCREAS AND LIVER IN PATINETS SUFFERING FROM CHRONIC BILIARY PANCREATITIS COMBINED WITH OBESITY
Authors: Kytsai K. Yu. , Babinets L. S 
Published: July 2017

Вступ. Статистика свідчить про зростання кількості огрядних людей протягом останніх років у цілому світі, незважаючи на підвищену увагу до цієї проблеми з боку наукової і практичної медицини. Ожиріння має системний характер і впливає на метаболізм і фізіологічні параметри.Мета роботи: проведення порівняльного аналізу показників жорсткості підшлункової залози та печінки у хворих на хронічний біліарний панкреатит (ХБП) в залежності від ступеня ожиріння.Матеріали і методи. Було обстежено 100 пацієнтів з ХП біліарного ґенезу.Групу контролю склали 20 молодих здорових людей. ІМТ обчислювали за формулою Кетле.Результати та їх обговорення. У пацієнтів хворих на ХБП із ІІІ ступенем ожиріння рівень жорсткості становив (11,81±0,28) кПа, що у 2,5 рази перевищувало такий групи контролю, а показник жорсткості ПЗ – у 2,0 раза. Показник жорсткості печінки і ПЗ у групі хворих із ІІ ступенем ожиріння перевищував такий групи контролю у 2,0 і 1,8 рази відповідно, а у пацієнтів з І ступенем – в 1,6 та 1,5 рази і у хворих з надмірною масою тіла - у 1,30 та 1,28.Висновки.У хворих на хронічний біліарний панкреатит в поєднанні з ожирінням було константовано, що із зростанням маси тіла збільшувались показники жорсткості як печінки, так і підшлункової залози.
ЗАСТОСУВАННЯ ВІЗУАЛЬНОГО АНАЛІЗУ АРТЕРІАЛЬНИХ ОСЦИЛОГРАМ В ПРАКТИЦІ СІМЕЙНОГО ЛІКАРЯ
Authors: Vakulenko D. V. , Vakulenko L. O. , Kutakova O. V. 
Published: July 2017

Запропоновані авторами інформаційні технології візуального аналізу артеріальних осцилограм значно підвищують інформативність процедури вимірювання артеріального тиску. Можуть бути використані для раннього виявлення донозологічних і преморбідних станів та функціональних резервів системи кровообігу, контролю ефективності лікування, які допоможуть лікарю більш ефективно спланувати профілактичний, діагностичний та терапевтичний процес.
ШЛЯХИ КОРЕКЦІЇ ОСТЕОДЕФІЦИТУ ПРИ ХРОНІЧНОМУ ПАНКРЕАТИТІ
Authors: Semenova I. V. , Babinets L. S. , Kotsaba Yu. Ya. , Kryskiv O. I. , Velyka O. B. , Zayec T. A. 
Published: July 2017

Резюме. Актуальність проблеми розвитку остеодефіцитних станів при хронічному панкреатиті зумовлена розладами процесів травлення, що виявляється синдромами мальдигестії та мальабсорбції з порушенням засвоєння мінеральних і органічних речовин. Ключовим моментом у розвитку остеодефіциту при хронічному панкреатиті, безумовно, є дефіцит кальцію та вітаміну D3. Остеодефіцит при ХП може мати компоненти як остеомаляції (внаслідок гіповітамінозу вітаміну D3), так і остеопорозу (порушення кальцієво-фосфорного, білкового обмінів вторинно, а також внаслідок первинного остеопорозу – вікового, постменопаузального). З метою оцінки стану мінеральної щільності кісткової тканини хворим проводили обстеження поперекового відділу хребта за допомогою двофотонного рентгенівського денситометра. Встановлено, що у 75 % обстежених хворих на хронічний панкреатит спостерігаються порушення мінералізації кісткової тканини.Проведені дослідження обґрунтовують доцільність спільного використання кальцієвмісного вітамінно-мінерального препарату та бісфосфонату ризендронової кислоти за запропонованою схемою у лікуванні супутнього остеопорозу у хворих на хронічний панкреатит. Доведено доцільність використання препаратів Вітрум Кальціум 600+D400 та Ризендрос в комплексному лікуванні хворих на хронічний панкреатит із супутнім остеодефіцитом, що призвело до суттєвого достовірного покращення стану мінералізації кістки - приросту мінеральної щільності кісткової тканини.
Selective Deletion of Leptin Signaling in Endothelial Cells Enhances Neointima Formation and Phenocopies the Vascular Effects of Diet-Induced Obesity in Mice
Authors: Hubert Astrid , Bochenek Magdalena L. , Schütz Eva , Gogiraju Rajinikanth , Münzel Thomas , Schäfer Katrin 
Published: July 2017

Objective—
Obesity is associated with elevated circulating leptin levels and hypothalamic leptin resistance. Leptin receptors (LepRs) are expressed on endothelial cells, and leptin promotes neointima formation in a receptor-dependent manner. Our aim was to examine the importance of endothelial LepR (End.LepR) signaling during vascular remodeling and to determine whether the cardiovascular consequences of obesity are because of hyperleptinemia or endothelial leptin resistance.

Approach and Results—
Mice with loxP-flanked LepR alleles were mated with mice expressing Cre recombinase controlled by the inducible endothelial receptor tyrosine kinase promoter. Obesity was induced with high-fat diet. Neointima formation was examined after chemical carotid artery injury. Morphometric quantification revealed significantly greater intimal hyperplasia, neointimal cellularity, and proliferation in End.LepR knockout mice, and similar findings were obtained in obese, hyperleptinemic End.LepR wild-type animals. Analysis of primary endothelial cells confirmed abrogated signal transducer and activator of transcription-3 phosphorylation in response to leptin in LepR knockout and obese LepR wild-type mice. Quantitative PCR, ELISA, and immunofluorescence analyses revealed increased expression and release of endothelin-1 in End.LepR-deficient and LepR-resistant cells, and ET receptor A/B antagonists abrogated their paracrine effects on murine aortic smooth muscle cell proliferation. Reduced expression of peroxisome proliferator-activated receptor-γ and increased nuclear activator protein-1 staining was observed in End.LepR-deficient and LepR-resistant cells, and peroxisome proliferator-activated receptor-γ antagonization increased endothelial endothelin-1 expression.

Conclusions—
Our findings suggest that intact endothelial leptin signaling limits neointima formation and that obesity represents a state of endothelial leptin resistance. These observations and the identification of endothelin-1 as soluble mediator of the cardiovascular risk factor obesity may have relevant therapeutic implications.