bluuebunny/crossref_metadata_2025_split huggingface dataset:
Double endoscopic bypass for gastric outlet obstruction and biliary obstruction
Authors: Brewer Gutierrez Olaya , Nieto Jose , Irani Shayan , James Theodore , Pieratti Bueno Renata , Chen Yen-I , Bukhari Majidah , Sanaei Omid , Kumbhari Vivek , Singh Vikesh , Ngamruengphong Saowanee , Baron Todd , Khashab Mouen 
Published: September 2017

Abstract
Background and study aims Double endoscopic bypass entails EUS-guided gastroenterostomy (EUS-GE) and EUS-guided biliary drainage (EUS-BD) in patients who present with gastric outlet and biliary obstruction. We report a multicenter experience with double endoscopic bypass.
Patients and methods Retrospective, multicenter series involving 3 US centers. Patients who underwent double endoscopic bypass for malignant gastric and biliary obstruction from 1/2015 to 12/2016 were included. Primary outcome was clinical success defined as tolerance of oral intake and resolution of cholestasis. Secondary outcomes included technical success, re-interventions and adverse events (AE).
Results Seven patients with pancreatic head cancer (57.1 % females; mean age 64.6 ± 12.5 years) underwent double endoscopic bypass. Four patients had EUS-GE and EUS-BD performed during the same session with a mean procedure time of 70 ± 20.4 minutes. EUS-GE and EUS-BD were technically successful in all patients, all of whom were able to tolerate oral intake with resolution of cholestasis in 6 (87.5 %). One patient had a repeat EUS-BD with normalization of bilirubin. There were no adverse events.
Conclusions Double endoscopic bypass is feasible and effective when performed by experienced operators. Studies comparing this novel concept to existing techniques are warranted.
Operative Verfahren in der Neurochirurgie und Neurotraumatologie
Authors: Röhrer Stefan , Kapapa Thomas 
Published: September 2017

ZusammenfassungAufgrund ausgeprägter und residualer neurologischer Störungen bedürfen neurochirurgische Erkrankungen oftmals einer rehabilitativen
Behandlung. Im vorliegenden Übersichtsartikel sollen die kraniellen neurochirurgischen Erkrankungen, die häufig zu einer rehabilitativen
Behandlung führen, näher erläutert werden.
Funktionelle Elektrostimulation als Therapie des neurogenen Fallfußes
Authors: Daniilidis Kiriakos , Stukenborg-Colsman Christina , Kellner Janina , Umbach Oliver , Yao Daiwei 
Published: September 2017

ZusammenfassungHäufige Folge eines Schlaganfalls ist eine Fußlähmung. Sie führt zu wesentlichen Beeinträchtigungen im Gangvorgang. Der Gang wird unsicher.
Der Betroffene ist meist gezwungen, den Kopf zu neigen, da er ein visuelles Feedback des Gehens benötigt. Neben chirurgischen und
konservativen Methoden hat sich in den letzten zehn Jahren die funktionelle Elektrostimulation als therapeutisches Therapiekonzept
bewiesen.
Same-day Routine Chest-X Ray After Thoracic Surgery is Not Necessary!
Authors: Nagy Peter , Antony Christiane , Hegedüs Balazs , Kampe Sandra , Ploenes Till , Aigner Clemens , Welter Stefan 
Published: September 2017

Abstract
Introduction Performing a routine postoperative chest X-ray (CXR) after general thoracic surgery is daily practice in many thoracic surgery departments. The quality, frequency of pathological findings and the clinical consequences have not been well evaluated. Furthermore, exposure to ionising radiation should be restricted to a minimum and therefore routine practice can be questioned.
Methods As a hospital standard, each patient was given a routine CXR after opening of the pleura and inserting a chest tube. From October 2015 to March 2016, each postoperative patient with a routine CXR was included in a prospective database, including film quality, pathological findings, clinical and laboratory results and cardiorespiratory monitoring, as well as clinical consequences.
Results 546 patients were included. Risk factors for postoperative complications were obesity in 50 patients (9.2%), emphysema in 127 patients (23.3%), coagulopathy in 34 patients (6.2%), longer operation time (more than two hours) in 242 patients (44.3%) and previous lung irradiation in 29 (5.3%) of patients. Major lung resections were performed in 191 patients (35.9%). 263 (48.2%) patients had procedures with minimally invasive access. The quality of the X-ray film was insufficient in 8.2% of patients. 90 (16.5%) of CXRs were found to show pathological findings, with a trend for more pathological findings after open surgery (55/283; 19.4%) compared to minimally invasive surgery (35/263; 13.3%) (p = 0.064). 11 (2.0%) patients needed a surgical or clinical intervention during postoperative observation; this corresponds to 12.2% of patients with a pathological finding on CXR. Nine of these 11 patients were clinically symptomatic and only two (0.37%) patients were asymptomatic with a relevant pneumothorax.
Conclusions Our study cannot support routine postoperative CXR after general thoracic procedures and we believe that restriction to clinically symptomatic cases should be a safe option.
Breeding for cuticle-associated traits in crop species: traits, targets, and strategies
Authors: Petit Johann , Bres Cécile , Mauxion Jean-Philippe , Bakan Bénédicte , Rothan Christophe 
Published: September 2017

AbstractImproving crop productivity and quality while promoting sustainable agriculture have become major goals in plant breeding. The cuticle is a natural film covering the aerial organs of plants and consists of lipid polyesters covered and embedded with wax. The cuticle protects plants against water loss and pathogens and affects traits with strong impacts on crop quality such as, for horticultural crops, fruit brightness, cracking, russeting, netting, and shelf life. Here we provide an overview of the most important cuticle-associated traits that can be targeted for crop improvement. To date, most studies on cuticle-associated traits aimed at crop breeding have been done on fleshy fruits. Less information is available for staple crops such as rice, wheat or maize. Here we present new insights into cuticle formation and properties resulting from the study of genetic resources available for the various crop species. Our review also covers the current strategies and tools aimed at exploiting available natural and artificially induced genetic diversity and the technologies used to transfer the beneficial alleles affecting cuticle-associated traits to commercial varieties.
Prevalence and Prognosis Impact of Patient–Ventilator Asynchrony in Early Phase of Weaning according to Two Detection Methods
Authors: Rolland-Debord Camille , Bureau Côme , Poitou Tymothee , Belin Lisa , Clavel Marc , Perbet Sébastien , Terzi Nicolas , Kouatchet Achille , Similowski Thomas , Demoule Alexandre 
Published: September 2017

Abstract

Background
Patient–ventilator asynchrony is associated with a poorer outcome. The prevalence and severity of asynchrony during the early phase of weaning has never been specifically described. The authors’ first aim was to evaluate the prognosis impact and the factors associated with asynchrony. Their second aim was to compare the prevalence of asynchrony according to two methods of detection: a visual inspection of signals and a computerized method integrating electromyographic activity of the diaphragm.

Methods
This was an ancillary study of a multicenter, randomized controlled trial comparing neurally adjusted ventilatory assist to pressure support ventilation. Asynchrony was quantified at 12, 24, 36, and 48 h after switching from controlled ventilation to a partial mode of ventilatory assistance according to the two methods. An asynchrony index greater than or equal to 10% defined severe asynchrony.

Results
A total of 103 patients ventilated for a median duration of 5 days (interquartile range, 3 to 9 days) were included. Whatever the method used for quantification, severe patient–ventilator asynchrony was not associated with an alteration of the outcome. No factor was associated with severe asynchrony. The prevalence of asynchrony was significantly lower when the quantification was based on flow and pressure than when it was based on the electromyographic activity of the diaphragm at 0.3 min–1 (interquartile range, 0.2 to 0.8 min–1) and 4.7 min–1 (interquartile range, 3.2 to 7.7 min–1; P < 0.0001), respectively.

Conclusions
During the early phase of weaning in patients receiving a partial ventilatory mode, severe patient–ventilator asynchrony was not associated with adverse clinical outcome, although the prevalence of patient–ventilator asynchrony varies according to the definitions and methods used for detection.
Risk of Knee Sepsis After Treatment of Open Tibia Fractures: A Multicenter Comparison of Suprapatellar and Infrapatellar Approaches
Authors: Marecek Geoffrey S. , Nicholson Luke T. , Broghammer Frances H. , Talerico Michael , Tougas Caroline , Donegan Derek J. , Scolaro John A. 
Published: September 2017

Objectives:
The suprapatellar approach for medullary nailing of the tibia is increasing. This requires intra-articular passage of instruments, which theoretically places the knee at risk of postoperative sepsis in the setting of open fracture. We hypothesized that the risk of knee sepsis is similar after suprapatellar or infrapatellar nailing of open tibia fractures.

Design:
Retrospective, multicenter.

Setting:
Three urban level 1 trauma centers.

Patients:
All patients treated with medullary nailing for open diaphyseal tibia fractures (OTA 42) from 2009 to 2015. Patients younger than 18 years of age and with less than 12 weeks of follow-up were excluded. We identified 289 fractures in 282 patients.

Intervention:
Suprapatellar (SP) or infrapatellar (IP) medullary nailing of open tibia fractures.

Main Outcome Measurement:
Occurrence of ipsilateral knee sepsis, defined as presence of a positive culture from knee aspiration or arthrotomy. Deep infection requiring operative debridement, superficial infection requiring antibiotic therapy alone, and all-cause reoperation were also recorded.

Results:
IP nailing was used for 142 fractures. There were 20 infections (14.1%), of which 14 (9.8%) were deep. Fourteen tibias (9.8%) required reoperation for noninfectious reasons for 28 total reoperations (19.7%). SP nailing was used in 147 fractures. There were 24 infections (16.2%), of which 16 (10.8%) were deep. Fourteen additional tibias (9.5%) required reoperation for noninfectious reasons for a total of 30 reoperations (20.4%). There were no differences in the rates of infection, deep infection, or reoperation between groups. There were 2 cases of knee sepsis after SP nailing (1.4%) but zero cases after IP nailing (P = 0.5).

Conclusions:
There was no significant difference in the rate of knee sepsis with SP or IP approaches. The risk of knee sepsis after SP nailing of open fractures is low, but present.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Is Contralateral Templating Reliable for Establishing Rotational Alignment During Intramedullary Stabilization of Femoral Shaft Fractures? A Study of Individual Bilateral Differences in Femoral Version
Authors: Croom William P. , Lorenzana Daniel J. , Auran Richard L. , Cavallero Matthew J. , Heckmann Nathanael , Lee Jackson , White Eric A. 
Published: September 2017

Objectives:
To determine native individual bilateral differences (IBDs) in femoral version in a diverse population.

Methods:
Computed tomography scans with complete imaging of uninjured bilateral femora were used to determine femoral version and IBDs in version. Age, sex, and ethnicity of each subject were also collected. Femoral version and IBDs in version were correlated with demographic variables using univariate and multivariate regression models.

Results:
One hundred sixty-four subjects were included in the study. The average femoral version was 9.4 degrees (±9.4 degrees). The mean IBD in femoral version was 5.4 degrees (±4.4 degrees, P < 0.001). A total of 17.7% of subjects had a difference in version ≥10 degrees, and 4.3% had a difference in version ≥15 degrees. A femur with anteversion ≥20 degrees or retroversion was associated with a greater mean difference in version from the contralateral side compared with those with midrange anteversion.

Conclusions:
Bilateral differences in femoral version are common and can result in a difference from native anatomy that may be clinically significant if only the contralateral limb is used to establish rotational alignment during intramedullary stabilization of diaphyseal femur fractures. This is also an important consideration when considering malrotation of femur fractures because most studies define malrotation as a greater than 10–15-degree difference compared with the contralateral side.

Level of Evidence:
Prognostic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Ergonomics in Surgery: A Review
Authors: Catanzarite Tatiana , Tan-Kim Jasmine , Whitcomb Emily L. , Menefee Shawn 
Published: September 2017

Objective
Work-related musculoskeletal disorders (WMSDs) are prevalent among surgeons and may result in practice modification. We aimed to perform a comprehensive review of the English-language literature regarding ergonomic risk, prevalence of WMSDs, and unique ergonomic considerations by route of surgery.

Methods
Multiple searches were performed of PubMed and University library resources to access English-language publications related to surgeon ergonomics. Combinations of keywords were used for each mode of surgery, including the following: “ergonomics,” “guidelines,” “injury,” “operating room,” “safety,” “surgeon,” and “work-related musculoskeletal disorders.” Each citation was read in detail, and references were reviewed.

Results
Surgeon WMSDs are prevalent, with rates ranging from 66% to 94% for open surgery, 73% to 100% for conventional laparoscopy, 54% to 87% for vaginal surgery, and 23% to 80% for robotic-assisted surgery. Risk factors for injury in open surgery include use of loupes, headlamps, and microscopes. Unique risks in laparoscopic surgery include table and monitor position, long-shafted instruments, and poor instrument handle design. In vaginal surgery, improper table height and twisted trunk position create injury risk. Although robotic surgery offers some advantages, it remains associated with trunk, wrist, and finger strain. Surgeon WMSDs often result in disability but are under-reported to institutions. Additionally, existing research tools face limitations in the operating room environment.

Conclusions
Work-related musculoskeletal disorders are prevalent among surgeons but have received little attention owing to under-reporting of injury and logistical constraints of studying surgical ergonomics. Future research must aim to develop objective surgical ergonomics instruments and guidelines and to correlate ergonomics assessments with pain and tissue-level damage in surgeons with WMSDs. Ergonomics training should be developed to protect surgeons from preventable, potentially career-altering injuries.
Comparison of gene expression patterns ofKappaphycus alvarezii(Rhodophyta, Solieriaceae) under different light wavelengths and CO2enrichment
Authors: Yee Thien Vun , Rodrigues Kenneth Francis , Wong Vui Ling Clemente Michael , Thau Lym Wilson Yong 
Published: September 2017

AbstractTranscriptomes associated with the process of photosynthesis and carbon fixation have offered insights into the mechanism of gene regulation in terrestrial plants, however limited information is available as far as macroalgae are concerned. Intertidal red alga,Kappaphycus alvareziiis exposed to different wavelengths of light in their lives as light quantity and quality changes at different depths in seawater. This investigation aims to study the underlying mechanisms associated with photosynthesis and carbon fixation under specific light qualities and CO2enrichment. Light regulation of gene expression has not been previously described for red algae. By using next generation sequencing, transcriptome profiling ofK. alvareziigenerated 76,871 qualified transcripts with a mean length of 979bp and a N50 length of 1,707bp and 55.83% transcripts were annotated on the basis of function. Blue, green and red light all have demonstrated roles in modulating light responses, such as changes in gene expression. Here we analysed the effects of light regulation on four selected photosynthesis aspects (light-harvesting complex, phycobilisomes, photosystems and photoreceptors). We observed that light-regulated gene expression in this species is not a single light response and different light qualities are transduced to regulate the same metabolic pattern. The carbon fixation pathway was analysed and key genes encoding enzymes involved in the carbon fixation pathway such as ppc, pepc, prk, pgk, ppdk, provided that unequivocal molecular evidence that most of the C3and C4pathway genes were actively transcribed inK. alvarezii. In addition to this the CO2induced transcriptome suggested the possibility of shifting carbon metabolism pathway after acclimation to increased level of CO2. Impact of CO2enrichment on the cultures has provided new insight into the response to rising CO2.
Systemic and vascular inflammation in an in-vitro model of central obesity
Authors: Ahluwalia A. , Misto A. , Vozzi F. , Magliaro C. , Mattei G. , Marescotti MC. , Avogaro A. , Iori E. 
Published: September 2017

AbstractMetabolic disorders due to over-nutrition are a major global health problem, often associated with obesity and related morbidities. Obesity is peculiar to humans, as it is associated with lifestyle and diet, and so difficult to reproduce in animal models.Here we describe a model of human central adiposity based on a 3-tissue system consisting of a series of interconnected fluidic modules. Given the causal link between obesity and systemic inflammation, we focused primarily on pro-inflammatory markers, examining the similarities and differences between the 3-tissue model and evidence from human studies in the literature. When challenged with high levels of adiposity, the in-vitro system manifests cardiovascular stress through expression of E-selectin and von Willebrand factor as well as systemic inflammation (expressing IL-6 and MCP-1) as observed in humans. Interestingly, most of the responses are dependent on the synergic interaction between adiposity and the presence of multiple tissue types. The set-up has the potential to reduce animal experiments in obesity research and may help unravel specific cellular mechanisms which underlie tissue response to nutritional overload.
A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits
Authors: Rouvière Jérôme O. , Bulfoni Manuel , Tuck Alex , Cosson Bertrand , Devaux Frédéric , Palancade Benoit 
Published: September 2017

SUMMARYWhile the activity of multiprotein complexes is crucial for cellular metabolism, little is known about the mechanisms that collectively control the expression of their components in response to the cellular demand. Here, we have investigated the regulations targeting the biogenesis of the nuclear pore complex (NPC), the macromolecular assembly mediating nucleocytoplasmic exchanges. Systematic analysis of RNA-binding proteins interactomes, together within vivoandin vitroassays, revealed that a subset ofNPCmRNAs are specifically bound by Hek2, a yeast hnRNP K-like protein. Hek2-dependentNPCmRNAs translational repression and protein turnover are further shown to finely tune the levels of NPC subunits. Strikingly, Hek2 binding to its target mRNAs requires prior desumoylation by the NPC-associated SUMO protease Ulp1. Mutations or physiological perturbations altering NPC integrity lead to a decrease in the levels of active Ulp1 and to the accumulation of sumoylated, inactive forms of Hek2. Our results support the existence of a quality control mechanism involving Ulp1 as a sensor of NPC integrity and Hek2 as a repressor of NPC biogenesis.
Characterizing highly dynamic conformational states: the transcription bubble in RNAP-promoter open complex as an example
Authors: Lerner Eitan , Ingargiola Antonino , Weiss Shimon 
Published: September 2017

ABSTRACTBio-macromolecules carry out complicated functions through structural changes. To understand their mechanism of action, the structure of each step has to be characterized. While classical structural biology techniques allow the characterization of a few ‘structural snapshots’ along the enzymatic cycle (usually of stable conformations), they do not cover all (and often fast interconverting) structures in the ensemble, where each may play an important functional role. Recently, several groups have demonstrated that structures of different conformations in solution could be solved by measuring multiple distances between different pairs of residues using single-molecule Förster resonance energy transfer (smFRET) and using them as constrains for hybrid/integrative structural modeling. However, this approach is limited in cases where the conformational dynamics is faster than the technique’s temporal resolution. In this study, we combine existing tools that elucidate sub-millisecond conformational dynamics together with hybrid/integrative structural modeling to study the conformational states of the transcription bubble in the bacterial RNA polymerase (RNAP)-promoter open complex (RPo). We measured microsecond alternating laser excitation μsALEX)-smFRET of differently labeled lacCONS promoter dsDNA constructs. We used a combination of burst variance analysis (BVA), photon-by-photon hidden Markov modelling (H2MM) and the FRET-restrained positioning and screening (FPS) approach to identify two conformational states for RPo. The experimentally-derived distances of one conformational state match the known crystal structure of bacterial RPo. The experimentally-derived distances of the other conformational state have characteristics of a scrunched RPo. These findings support the hypothesis that sub-millisecond dynamics in the transcription bubble are responsible for transcription start site selection.
User evaluation of a task for shortlisting papers from researcher’s reading list for citing in manuscripts
Authors: Sesagiri Raamkumar Aravind , Foo Schubert , Pang Natalie 
Published: September 2017

Purpose
Although many interventional approaches have been proposed to address the apparent gap between novices and experts for literature review (LR) search tasks, there have been very few approaches proposed for manuscript preparation (MP) related tasks. The purpose of this paper is to describe a task and an incumbent technique for shortlisting important and unique papers from the reading list (RL) of researchers, meant for citation in a manuscript.

Design/methodology/approach
A user evaluation study was conducted on the prototype system which was built for supporting the shortlisting papers (SP) task along with two other LR search tasks. A total of 119 researchers who had experience in authoring research papers participated in this study. An online questionnaire was provided to the participants for evaluating the task. Both quantitative and qualitative analyses were performed on the collected evaluation data.

Findings
Graduate research students prefer this task more than research and academic staff. The evaluation measures relevance, usefulness and certainty were identified as predictors for the output quality measure “good list”. The shortlisting feature and information cues were the preferred aspects while limited data set and rote steps in the study were ascertained as critical aspects from the qualitative feedback of the participants.

Originality/value
Findings point out that researchers are clearly interested in this novel task of SP from the final RL prepared during LR. This has implications for digital library, academic databases and reference management software where this task can be included to benefit researchers at the manuscript preparatory stage of the research lifecycle.