bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a patient experience questionnaire to improve lifestyle services in primary care
Authors: Brauer Paula , Royall Dawna , Kaethler Anneli , Mayhew Alexandra , Israeloff-Smith Maya 
Published: January 2018

AimWe developed a self-report tool to assess patients’ experiences with lifestyle services in team-based primary care and pilot tested the questionnaire as part of a lifestyle intervention study to reverse metabolic syndrome.BackgroundOlder client satisfaction questionnaires have been generally inadequate for quality improvement purposes, as they have been focused mainly on interpersonal skills of providers and/or in the context of one disease. New approaches to assessing the patient experience of lifestyle programs in primary care are needed and could inform quality improvement efforts over time.MethodsThe first phase in developing the questionnaire involved a group-administered questionnaire distributed to 38 healthcare providers in five groups to prioritize variables to include in the survey. Concepts were taken from a previous review of available questionnaires assessing primary care services. The draft questionnaire was reviewed by 11 participants from a lifestyle program using think-aloud cognitive interviewing techniques. The modified self-administered questionnaire (paper and online versions) was then pilot tested with 164 recipients of a nutrition and physical activity intervention program.FindingsProviders ranked the top variables to include in the questionnaire as: ‘trust,’ ‘general communication,’ ‘first-contact accessibility,’ ‘whole-person care,’ and ‘respectfulness.’ After cognitive interviewing and revisions, 21 multiple choice and two open-ended questions were used for pilot testing. Pilot testing identified additional minor wording changes that were needed for clarity, a decreased number of questions for redundant concepts, and decreased options for ceiling effects, resulting in 20 multiple choice and one open-ended question.ConclusionsThe modified self-administered patient experience questionnaire to assess lifestyle services in primary care has undergone rigorous development. Further validation is needed. The assessment of patient experience of lifestyle programs can be used to supplement other data to assess the overall effectiveness of such programs.
Have You Voted? Teaching OSCOLA with Mentimeter
Authors: Skoyles Alison , Bloxsidge Erin 
Published: January 2018

AbstractThis article details a small project undertaken by Information Skills Advisers at London South Bank University to increase student engagement in lecture theatre settings through the use of a web-based audience response system (ARS), Mentimeter. Supporting the School of Law, librarians delivered an interactive OSCOLA referencing session to a large capacity lecture theatre. A previous session was redesigned to increase student engagement and active learning utilising interactive quiz questions which students answered using mobile devices. The majority of feedback from students and staff who participated in this pilot project was positive and showed an increase in student engagement. This article is based on the presentation, ‘Have You Voted?: Teaching OSCOLA with Mentimeter’ delivered at the BIALL Annual Conference 2017. It will examine the process of adapting a lecture to include interactive content using an ARS, and will discuss the outcomes of the pilot session and explore benefits and challenges inherent in using technology of this type in a large classroom.
Biting midges (Diptera: Ceratopogonidae) as indicators of biostratigraphy, ecological reconstructions and identification of amber deposits
Authors: Szadziewski Ryszard 
Published: January 2018

ABSTRACTBiting midges (Diptera: Ceratopogonidae) are a large family of flies that commononly appear in Lower Cretaceous to Miocene strata, with over 280 fossil species (4.3 % of the family), belonging to 49 genera (26 extant; 23 extinct). Morphological characters used in the identification of fossil genera and species are identical to those used in studies of extant Ceratopogonidae and, as a result, their potential indicative value is reliable. Two relictual extant genera,Leptoconopsand Austroconops, reported from Lower Cretaceous Lebanese amber, are at least 125 million years old. Certain ceratopogonid genera are indicative for the Lower Cretaceous, Upper Cretaceous, Eocene or Miocene. A morphological character indicative for the Upper Cretaceous and Cenozoic is macrotrichia on the wing membrane of adults. Indicator species and genera are reviewed for all amber deposits. Eocene Baltic amber contains the best known fauna of biting midges, with 109 named species. Some genera are indicative of aquatic and semiaquatic habitats (predaceous genera, subfamily Ceratopogoninae), forests with rotting trees (Forcipomyia), sandy sea shore habitats (Leptoconops), a cold boreal climate (Ceratopogon) or warm climates (Nannohelea,Austroconops,Leptoconops,Meunierohelea,Metahelea). Females require a protein-rich meal and are well known for feeding on the blood of vertebrates, but many feed on other things, so this information can help with the interpretation of palaeoenvironments.Washingtonhelea taimyricaSzadziewski, 1996, described from Siberian amber, is transferred to the fossil genusPalaeobrachypogon:P.taimyricus(Szadziewski, 1996), comb. nov. ForSerromyia alphea,mistakenly redescribed and illustrated from Eocene Bitterfeld amber (= Baltic amber) by Szadziewski (1993), a new name –SerromyiaerrataSzadziewski, nom. nov. – is proposed.
New palaeodictyopterans from the Late Carboniferous of the UK (Insecta: Palaeodictyopterida)
Authors: Prokop Jakub , Pecharová Martina , Jarzembowski Edmund A. , Ross Andrew J. 
Published: January 2018

ABSTRACTNew palaeodictyopterans,Vernooijia sassoonaegen.etsp. nov. (Breyeriidae) andMazonopterumcooperisp. nov. (Homoiopteridae) are described from the Middle Pennsylvanian (Westphalian D/Late Asturian) of Writhlington, near Radstock (UK). Based on the re-examination of venation inBreyeriaharlemensis, we propose the transfer of this species to the genusVernooijiaasV.harlemensis(Brauckmann & Gröning, 1996) comb. nov. We report the first record ofHomaloneurasp. (Spilapteridae) from the Langsettian to Duckmantian of Coseley, Staffordshire. Additionally, we report a fragmentary wing from the Middle Pennsylvanian (late Westphalian D/early Cantabrian) of the Forest of Dean, Gloucestershire, tentatively belonging to the Lycocercidae. Our re-examination of a putative blattodean nymph described by Rolfe (1967) allows re-assignment to Palaeodictyoptera, as it has well-developed wing pads with a corrugated pattern of probably original tracheation and lacunal channels, identified as presumably nymphal exuvia ofIdoptilussp. Surprisingly, our study reveals the presence of three triangular caudal appendages bearing prominent lateral lamellae emerging from the terminal abdominal segment, previously unknown in other nymphs of Palaeodictyoptera. We assume that these lamellae were originally covered with dense setae and possibly represent modified caudal appendages in the form of tracheal gills, as known in the nymphs of damselflies (Odonata: Zygoptera). Thus, the scenario of a possible aquatic lifestyle for nymphs of at least some members of Palaeodictyoptera, as considered by Brongniart (1885, 1893) and Handlirsch (1906), cannot be definitely excluded.
Development of Coping Strategies From Childhood to	Adolescence
Authors: Eschenbeck Heike , Schmid Steffen , Schröder Ines , Wasserfall Nicola , Kohlmann Carl-Walter 
Published: January 2018

Abstract. Extensive research exists on coping in children and adolescents. However, developmental issues have only recently started to receive more attention. The present study examined age differences and developmental changes in six coping strategies (social support seeking, problem solving, avoidant coping, palliative emotion regulation, anger-related emotion regulation, and media use) assessed by a coping questionnaire (German Stress and Coping Questionnaire for Children and Adolescents, SSKJ 3–8; Lohaus, Eschenbeck, Kohlmann, & Klein-Heßling, 2006 ) in middle/late childhood and early adolescence. At the initial assessment, 917 children from grades 3 to 7 (age range 8–15 years) were included (cross-sectional sample). Three cohorts (grades 3–5 at baseline) were traced longitudinally over 1½ years with four assessments (longitudinal sample: n = 388). The cross-sectional coping data showed significant effects for grade level in four coping strategies. Older children scored higher in problem solving and media use, and lower in avoidant coping. Seventh graders scored lower than fourth and fifth graders in social support seeking. Longitudinal data confirmed time effects and cohort effects indicating developmental changes. Increases over time were found for problem solving and media use; decreases were found for avoidant coping and anger-related emotion regulation. For social support seeking, an increase within the youngest cohort (grades 3–5) was found. Developmental trends (in cross-sectional and longitudinal data), with especially strong increases for problem solving or declines in avoidant coping in the youngest cohort, differed for the two studied stressful situations (social, academic) but were independent of the child’s gender. To conclude, particularly in the age range of 9–11 years relevant developmental changes toward a more active coping seem to appear.
Low-cost and cleanroom-free fabrication of microneedles
Authors: Nejad Hojatollah Rezaei , Sadeqi Aydin , Kiaee Gita , Sonkusale Sameer 
Published: January 2018

AbstractWe present a facile, low-cost and cleanroom-free technique for the fabrication of microneedles using molds created by laser ablation. Microneedle mold with high aspect ratios is achieved on acrylic sheet by engraving a specific pattern of crossover lines (COL) using CO2 laser cutter. Ablating COL pattern on the acrylic sheet creates a sharp conical shape in the center of the design. We have shown that a variety of microneedle shapes with different heights and tip angles can be easily achieved by changing the number and the length of the COL. Polydimethylsiloxane (PDMS) microneedles were fabricated by casting the PDMS on the mold. The resulted PDMS microneedles are oxygen plasma treated and then silanized. Another PDMS layer is casted on PDMS microneedles and detached after curing. The silanization prevents those two layers of PDMS from bonding to each other and makes them easily detachable. After detachment of the PDMS mold of microneedles, the mold is used to fabricate degradable polyvinyl alcohol microneedle patch suitable for transdermal drug delivery. The release kinetics of the needles are also shown and discussed in order to prove the applicability of the needles.
Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo
Authors: Furuya Masayuki , Kikuta Junichi , Fujimori Sayumi , Seno Shigeto , Maeda Hiroki , Shirazaki Mai , Uenaka Maki , Mizuno Hiroki , Iwamoto Yoriko , Morimoto Akito , Hashimoto Kunihiko , Ito Takeshi , Isogai Yukihiro , Kashii Masafumi , Kaito Takashi , Ohba Shinsuke , Chung Ung-il , Lichtler Alexander C. , Kikuchi Kazuya , Matsuda Hideo , Yoshikawa Hideki , Ishii Masaru 
Published: January 2018

AbstractBone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial–temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell–cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell–cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell–cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo.
Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA
Authors: Rehage Nina , Davydova Elena , Conrad Christine , Behrens Gesine , Maiser Andreas , Stehklein Jenny E. , Brenner Sven , Klein Juliane , Jeridi Aicha , Hoffmann Anne , Lee Eunhae , Dianzani Umberto , Willemsen Rob , Feederle Regina , Reiche Kristin , Hackermüller Jörg , Leonhardt Heinrich , Sharma Sonia , Niessing Dierk , Heissmeyer Vigo 
Published: January 2018

AbstractThe ubiquitously expressed RNA-binding proteins Roquin-1 and Roquin-2 are essential for appropriate immune cell function and postnatal survival of mice. Roquin proteins repress target mRNAs by recognizing secondary structures in their 3′-UTRs and by inducing mRNA decay. However, it is unknown if other cellular proteins contribute to target control. To identify cofactors of Roquin, we used RNA interference to screen ~1500 genes involved in RNA-binding or mRNA degradation, and identified NUFIP2 as a cofactor of Roquin-induced mRNA decay. NUFIP2 binds directly and with high affinity to Roquin, which stabilizes NUFIP2 in cells. Post-transcriptional repression of human ICOS by endogenous Roquin proteins requires two neighboring non-canonical stem-loops in the ICOS 3′-UTR. This unconventional cis-element as well as another tandem loop known to confer Roquin-mediated regulation of the Ox40 3′-UTR, are bound cooperatively by Roquin and NUFIP2. NUFIP2 therefore emerges as a cofactor that contributes to mRNA target recognition by Roquin.
The Chemical Fluctuation Theorem governing gene expression
Authors: Park Seong Jun , Song Sanggeun , Yang Gil-Suk , Kim Philip M. , Yoon Sangwoon , Kim Ji-Hyun , Sung Jaeyoung 
Published: January 2018

AbstractGene expression is a complex stochastic process composed of numerous enzymatic reactions with rates coupled to hidden cell-state variables. Despite advances in single-cell technologies, the lack of a theory accurately describing the gene expression process has restricted a robust, quantitative understanding of gene expression variability among cells. Here we present the Chemical Fluctuation Theorem (CFT), providing an accurate relationship between the environment-coupled chemical dynamics of gene expression and gene expression variability. Combined with a general, accurate model of environment-coupled transcription processes, the CFT provides a unified explanation of mRNA variability for various experimental systems. From this analysis, we construct a quantitative model of transcription dynamics enabling analytic predictions for the dependence of mRNA noise on the mRNA lifetime distribution, confirmed against stochastic simulation. This work suggests promising new directions for quantitative investigation into cellular control over biological functions by making complex dynamics of intracellular reactions accessible to rigorous mathematical deductions.
Environmental enrichment increases transcriptional and epigenetic differentiation between mouse dorsal and ventral dentate gyrus
Authors: Zhang Tie-Yuan , Keown Christopher L. , Wen Xianglan , Li Junhao , Vousden Dulcie A. , Anacker Christoph , Bhattacharyya Urvashi , Ryan Richard , Diorio Josie , O’Toole Nicholas , Lerch Jason P. , Mukamel Eran A. , Meaney Michael J. 
Published: January 2018

AbstractEarly life experience influences stress reactivity and mental health through effects on cognitive-emotional functions that are, in part, linked to gene expression in the dorsal and ventral hippocampus. The hippocampal dentate gyrus (DG) is a major site for experience-dependent plasticity associated with sustained transcriptional alterations, potentially mediated by epigenetic modifications. Here, we report comprehensive DNA methylome, hydroxymethylome and transcriptome data sets from mouse dorsal and ventral DG. We find genome-wide transcriptional and methylation differences between dorsal and ventral DG, including at key developmental transcriptional factors. Peripubertal environmental enrichment increases hippocampal volume and enhances dorsal DG-specific differences in gene expression. Enrichment also enhances dorsal-ventral differences in DNA methylation, including at binding sites of the transcription factor NeuroD1, a regulator of adult neurogenesis. These results indicate a dorsal-ventral asymmetry in transcription and methylation that parallels well-known functional and anatomical differences, and that may be enhanced by environmental enrichment.
Adaptive design of an X-ray magnetic circular dichroism spectroscopy experiment with Gaussian process modelling
Authors: Ueno Tetsuro , Hino Hideitsu , Hashimoto Ai , Takeichi Yasuo , Sawada Masahiro , Ono Kanta 
Published: January 2018

AbstractSpectroscopy is a widely used experimental technique, and enhancing its efficiency can have a strong impact on materials research. We propose an adaptive design for spectroscopy experiments that uses a machine learning technique to improve efficiency. We examined X-ray magnetic circular dichroism (XMCD) spectroscopy for the applicability of a machine learning technique to spectroscopy. An XMCD spectrum was predicted by Gaussian process modelling with learning of an experimental spectrum using a limited number of observed data points. Adaptive sampling of data points with maximum variance of the predicted spectrum successfully reduced the total data points for the evaluation of magnetic moments while providing the required accuracy. The present method reduces the time and cost for XMCD spectroscopy and has potential applicability to various spectroscopies.
Effects of genetic variants on platelet reactivity and one-year clinical outcomes after percutaneous coronary intervention: A prospective multicentre registry study
Authors: Joo Hyung Joon , Ahn Sung Gyun , Park Jae Hyoung , Park Ji Young , Hong Soon Jun , Kim Seok-Yeon , Choi WoongGil , Gwon HyeonCheol , Lim Young-Hyo , Kim Weon , Kang Woong Chol , Cho Yun-Hyeong , Kim Yong Hoon , Yoon JungHan , Shin WonYong , Hong Myeong-Ki , Garg Scot , Jang Yangsoo , Lim Do-Sun 
Published: January 2018

AbstractClopidogrel is the mainstay for antiplatelet treatment after percutaneous coronary intervention (PCI). The relationship of platelet reactivity and genetic polymorphism with clinical outcomes with newer-generation drug-eluting stents is unclear. We analysed 4,587 patients for the most powerful single-nucleotide polymorphisms (CYP2C19, CYP2C9, ABCB1, PON1, and P2Y12) related to on-treatment platelet reactivity (OPR). The optimal cut-off value of high OPR for major adverse thrombotic events was 266. CYP2C19 was significantly associated with high OPR and the number of CYP2C19*R (*2 or *3) alleles was proportional to the increased risk of high OPR. Death, myocardial infarction (MI), stroke, stent thrombosis, and bleeding events were assessed during a 1-year follow-up period. Primary endpoints were death and non-fatal MI. The cumulative 1-year incidence of death and stent thrombosis was significantly higher in patients with CYP2C19*2/*2, CYP2C19*2/*3, and CYP2C19*3/*3 (Group 3) than in patients with CYP2C19*1/*1 (Group 1). Multivariate Cox proportional hazard model showed that cardiac death risk was significantly higher in Group 3 than in Group 1 (hazard ratio 2.69, 95% confidence interval 1.154–6.263, p = 0.022). No association was reported between bleeding and OPR. Thus, CYP2C19 may exert a significant impact on the prognosis of PCI patients even in the era of newer-generation drug-eluting stents.
Tumor Tissue Detection using Blood-Oxygen-Level-Dependent Functional MRI based on Independent Component Analysis
Authors: Huang Huiyuan , Lu Junfeng , Wu Jinsong , Ding Zhongxiang , Chen Shuda , Duan Lisha , Cui Jianling , Chen Fuyong , Kang Dezhi , Qi Le , Qiu Wusi , Lee Seong-Whan , Qiu ShiJun , Shen Dinggang , Zang Yu-Feng , Zhang Han 
Published: January 2018

AbstractAccurate delineation of gliomas from the surrounding normal brain areas helps maximize tumor resection and improves outcome. Blood-oxygen-level-dependent (BOLD) functional MRI (fMRI) has been routinely adopted for presurgical mapping of the surrounding functional areas. For completely utilizing such imaging data, here we show the feasibility of using presurgical fMRI for tumor delineation. In particular, we introduce a novel method dedicated to tumor detection based on independent component analysis (ICA) of resting-state fMRI (rs-fMRI) with automatic tumor component identification. Multi-center rs-fMRI data of 32 glioma patients from three centers, plus the additional proof-of-concept data of 28 patients from the fourth center with non-brain musculoskeletal tumors, are fed into individual ICA with different total number of components (TNCs). The best-fitted tumor-related components derived from the optimized TNCs setting are automatically determined based on a new template-matching algorithm. The success rates are 100%, 100% and 93.75% for glioma tissue detection for the three centers, respectively, and 85.19% for musculoskeletal tumor detection. We propose that the high success rate could come from the previously overlooked ability of BOLD rs-fMRI in characterizing the abnormal vascularization, vasomotion and perfusion caused by tumors. Our findings suggest an additional usage of the rs-fMRI for comprehensive presurgical assessment.
Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing
Authors: Akiyama Tomohiko , Sato Saeko , Chikazawa-Nohtomi Nana , Soma Atsumi , Kimura Hiromi , Wakabayashi Shunichi , Ko Shigeru B. H. , Ko Minoru S. H. 
Published: January 2018

AbstractDirect generation of skeletal muscle cells from human pluripotent stem cells (hPSCs) would be beneficial for drug testing, drug discovery, and disease modelling in vitro. Here we show a rapid and robust method to induce myogenic differentiation of hPSCs by introducing mRNA encoding MYOD1 together with siRNA-mediated knockdown of POU5F1 (also known as OCT4 or OCT3/4). This integration-free approach generates functional skeletal myotubes with sarcomere-like structure and a fusion capacity in several days. The POU5F1 silencing facilitates MYOD1 recruitment to the target promoters, which results in the significant activation of myogenic genes in hPSCs. Furthermore, deep sequencing transcriptome analyses demonstrated that POU5F1-knockdown upregulates the genes associated with IGF- and FGF-signaling and extracellular matrix that may also support myogenic differentiation. This rapid and direct differentiation method may have potential applications in regenerative medicine and disease therapeutics for muscle disorders such as muscular dystrophy.
Parturition in baboons (PAPIO SPP.)
Authors: Schlabritz-Loutsevitch N. , Maher J. , Sullivan R. , Mari G. , Schenone M. , Cohen H. L. , Word R. A. , Hubbard G. B. , Dick E. J. 
Published: January 2018

AbstractThe Old World non-human primates (NHP) - baboons (Papio spp.) share similarities with humans regarding fetal and placental development and some pregnancy-related complications. Information about the mechanism of birth and complications arising during parturition in these species is relatively sparse. In this manuscript, we add information from a series of pathological and observational cases to highlight insights and selected complications of birth in Papio spp, based on video-recording of the delivery process, X-ray, MRI, and ultrasound evaluations in pregnant baboons. Additionally, we abstracted pathology records obtained from perinatal loss in a large baboon colony during a 17 year period. The presented cases provide important information for the management of pregnancy and delivery in Papio spp.