bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors Associated With Levels of Physical Activity in Chronic Kidney Disease Patients Undergoing Hemodialysis: The Role of Dialysis Versus Nondialysis Day
Authors: da Costa Rosa Clara Suemi , Nishimoto Danilo Yuzo , Júnior Ismael Forte Freitas , Ciolac Emmanuel Gomes , Monteiro Henrique Luiz 
Published: May 2017

Background:Patients on hemodialysis (HD) report lower physical activity (PA) levels. We analyzed factors associated with low levels of PA in patients with chronic kidney disease (CKD) and compared PA on HD day and non-HD.Methods:79 patients wore an accelerometer and were classified according to time spent on moderate-to-vigorous PA (MVPA). Demographic data, BMI, comorbidities, clinical status, and health-related quality of life (HRQoL) were checked for association with PA. In addition, PA level was compared between days of HD and non-HD.Results:Accelerometer compliance was 78.5% [33 men and 29 women (53.96 ± 15.71 yrs) were included in analysis]. 35.5% of sample achieved ≥150min/week on MVPA. Lower MVPA was associated with older age (OR = 5.80, 95% CI = 1.11 to 30.19, P = .04), and lower score of physical function HRQoL (OR = 4.33, 95% CI = 1.23 to 15.23, P = .02). In addition, patients spent 9.73% more time on sedentary behavior, 38.9% less on light PA and 74.9% less on MVPA on HD day versus non-HD day.Conclusion:Age and physical function HRQoL were the main factors associated to lower PA levels. In addition, lower time spent on PA during HD day suggest that strategies for increasing physical activity levels during HD day such exercising during HD session could help CKD patients to reach current PA recommendations.
Neighborhood Built Environment and Socioeconomic Status in Relation to Active Commuting to School in Children
Authors: Molina-García Javier , Queralt Ana 
Published: May 2017

Background:The role of neighborhood type in active commuting to school (ACS) has not been extensively studied in children. The purpose of this study was to analyze the association between neighborhood built environment (walkability) and neighborhood socioeconomic status (SES) with ACS among children.Methods:A cross-sectional study of 310 Spanish children (aged 10–12 y; 51% male) was conducted in 2015. Walkability was defined as an index of 3 built environment characteristics (ie, residential density, land-use mix, and street connectivity) based on geographical information system data. Children’s home and school neighborhoods were evaluated. ACS was evaluated by questionnaire. Mixed model regression analyses evaluated ACS in relation to neighborhood walkability and SES.Results:There were no significant SES–walkability interactions for ACS. Children living in more walkable neighborhoods reported 2.5 more trips per week compared with those living in less walkable neighborhoods (P < .001). Children attending schools located in lower SES neighborhoods reported more ACS trips per week than those attending schools in higher SES neighborhoods (P < .05).Conclusions:Home-neighborhood walkability and school-neighborhood SES were associated with ACS. This study highlights the importance of assessing children’s home environment and school environment when ACS behavior is analyzed.
Relationship Between Lower-Extremity Strength and Subjective Function in Individuals With Patellofemoral Pain
Authors: Glaviano Neal R. , Saliba Susan 
Published: May 2017

Objective: Evaluate the relationship between subjective knee function and lower-extremity strength in individuals with patellofemoral pain (PFP). Design: Cohort. Setting: Laboratory. Participants: Participants were 30 individuals with PFP (20 females and 10 males; 76.02 [17.88] kg, 173.04 [7.58] cm, and 24.9 [7] y). Main Outcome Measures: Subjects completed the Activities of Daily Living Scale (ADLS) and had lower-extremity hip and knee isometric strength assessed. Strength was compared between low and high subjective functioning ADLS groups. Correlations for strength and subjective function were assessed, with a linear regression utilized to determine if strength predicted subjective function. Results: Quadriceps strength was significantly greater in the high subjective function group (38.5 [13.9] percent body mass) than in the low subjective function group (27.88 [8.96] percent body mass, P = .02). Significant correlations were seen between the ADLS and all 5 lower-extremity strength measures (r = .376–.535). Quadriceps strength was a strong predictor of subjective function in those with PFP, explaining 28.6% of the total variance in the ADLS. Conclusions: Quadriceps strength was a strong predictor of subjective function when assessed by the ADLS in patients with PFP and significantly greater in those with higher subjective function. A strong relationship exists between self-reported function and lower-extremity strength, suggesting the need to evaluate and treat lower-extremity weakness.
The Effects of Blood Flow Restricted Electrostimulation on Strength and Hypertrophy
Authors: Slysz Joshua T. , Burr Jamie F. 
Published: May 2017

Context: The combined effect of neuromuscular electrical stimulation (NMES) and blood flow restriction (BFR) on muscle mass and strength has not been thoroughly investigated. Objective: To examine the effects of combined and independent BFR and a low-intensity NMES on skeletal muscle adaptation. Design: Exploratory study. Setting: Laboratory. Participants: Twenty recreationally active subjects. Main Outcome Measures: Subjects had each leg randomly allocated to 1 of 4 possible intervention groups: (1) cyclic BFR alone, (2) NMES alone, (3) BFR + NMES, or (4) control. Each leg was stimulated in its respective intervention group for 32 minutes, 4 days per week for 6 weeks. Mean differences in size (in grams) and isometric strength (in kilograms), between week 0 and week 6, were calculated for each group. Results: Leg strength increased 32 (19) kg in the BFR + NMES group, which differed from the 3 (11) kg change in the control group (P = .03). The isolated NMES and BFR groups revealed increases of 16 (28) kg and 18 (17) kg, respectively, but these did not statistically differ from the control, or one another. No alterations were statistically significant for leg size. Conclusion: Compared with a control that received no treatment, the novel combination of BFR and NMES led to increasing muscular strength of the knee extensors, but not muscle mass which had a large interindividual variability in response.
Population Genomic Analysis of 1,777 Extended-Spectrum Beta-Lactamase-Producing
Klebsiella pneumoniae
Isolates, Houston, Texas: Unexpected Abundance of Clonal Group 307
Authors: Long S. Wesley , Olsen Randall J. , Eagar Todd N. , Beres Stephen B. , Zhao Picheng , Davis James J. , Brettin Thomas , Xia Fangfang , Musser James M. 
Published: May 2017

ABSTRACT

Klebsiella pneumoniae
is a major human pathogen responsible for high morbidity and mortality rates. The emergence and spread of strains resistant to multiple antimicrobial agents and documented large nosocomial outbreaks are especially concerning. To develop new therapeutic strategies for
K. pneumoniae
, it is imperative to understand the population genomic structure of strains causing human infections. To address this knowledge gap, we sequenced the genomes of 1,777 extended-spectrum beta-lactamase-producing
K. pneumoniae
strains cultured from patients in the 2,000-bed Houston Methodist Hospital system between September 2011 and May 2015, representing a comprehensive, population-based strain sample. Strains of largely uncharacterized clonal group 307 (CG307) caused more infections than those of well-studied epidemic CG258. Strains varied markedly in gene content and had an extensive array of small and very large plasmids, often containing antimicrobial resistance genes. Some patients with multiple strains cultured over time were infected with genetically distinct clones. We identified 15 strains expressing the New Delhi metallo-beta-lactamase 1 (NDM-1) enzyme that confers broad resistance to nearly all beta-lactam antibiotics. Transcriptome sequencing analysis of 10 phylogenetically diverse strains showed that the global transcriptome of each strain was unique and highly variable. Experimental mouse infection provided new information about immunological parameters of host-pathogen interaction. We exploited the large data set to develop whole-genome sequence-based classifiers that accurately predict clinical antimicrobial resistance for 12 of the 16 antibiotics tested. We conclude that analysis of large, comprehensive, population-based strain samples can assist understanding of the molecular diversity of these organisms and contribute to enhanced translational research.

IMPORTANCE
Klebsiella pneumoniae
causes human infections that are increasingly difficult to treat because many strains are resistant to multiple antibiotics. Clonal group 258 (CG258) organisms have caused outbreaks in health care settings worldwide. Using a comprehensive population-based sample of extended-spectrum beta-lactamase (ESBL)-producing
K. pneumoniae
strains, we show that a relatively uncommon clonal type, CG307, caused the plurality of ESBL-producing
K. pneumoniae
infections in our patients. We discovered that CG307 strains have been abundant in Houston for many years. As assessed by experimental mouse infection, CG307 strains were as virulent as pandemic CG258 strains. Our results may portend the emergence of an especially successful clonal group of antibiotic-resistant
K. pneumoniae
.
The systolic paradox in hypertrophic cardiomyopathy
Authors: Haland Trine F , Hasselberg Nina E , Almaas Vibeke Marie , Dejgaard Lars A , Saberniak Jørg , Leren Ida S , Berge Knut Erik , Haugaa Kristina H , Edvardsen Thor 
Published: May 2017

ObjectiveWe explored cardiac volumes and the effects on systolic function in hypertrophic cardiomyopathy (HCM) patients with left ventricular hypertrophy (HCM LVH+) and genotype-positive patients without left ventricular hypertrophy (HCM LVH−).MethodsWe included 180 HCM LVH+, 100 HCM LVH− patients and 80 healthy individuals. End-Diastolic Volume Index (EDVI), End-Systolic Volume Index (ESVI) and ejection fraction (EF) were assessed by echocardiography. Left ventricular (LV) global longitudinal strain (GLS) was measured by speckle tracking echocardiography.ResultsEDVI and ESVI were significantly smaller in HCM LVH+ compared with HCM LVH− patients (41±14 mL/m2vs 49±13 mL/m2and 16±7 mL/m2vs 19±6 mL/m2, respectively, both p<0.001) and in healthy individuals (41±14 mL/m2vs 57±14 mL/m2and 16±7 mL/m2vs 23±9 mL/m2, respectively, both p<0.001). HCM LVH− patients had significantly lower EDVI and ESVI compared with healthy individuals (49±13 mL/m2vs 57±14 mL/m2and 19±6 mL/m2vs 23±9 mL/m2, both p<0.001). EF was similar (61%±7% vs 60%±8% vs 61%±6%, p=0.43) in the HCM LVH+, HCM LVH– and healthy individuals, despite significantly worse GLS in the HCM LVH+ (−16.4%±3.7% vs −21.3%±2.4% vs −22.3%±3.7%, p<0.001). GLS was worse in the HCM LVH− compared with healthy individuals in pairwise comparison (p=0.001). Decrease in ESVI was closely related to EF in HCM LVH+ and HCM LVH− (R=0.45, p<0.001 and R=0.43, p<0.001) as expected, but there was no relationship with GLS (R=0.02, p=0.77 and R=0.11, p=0.31). Increased maximal wall thickness (MWT) correlated significantly with worse GLS (R=0.58, p<0.001), but not with EF (R=0.018, p=0.30) in the HCM LVH+ patients.ConclusionHCM LVH+ had smaller cardiac volumes that could explain the preserved EF, despite worse GLS that was closely related to MWT. HCM LVH− had reduced cardiac volumes and subtle changes in GLS compared with healthy individuals, indicating a continuum of both volumetric and systolic changes present before increased MWT.
Clinical and cost-effectiveness of adapted cognitive behaviour therapy for non-cardiac chest pain: a multicentre, randomised controlled trial
Authors: Tyrer Peter , Tyrer Helen , Morriss Richard , Crawford Michael , Cooper Sylvia , Yang Min , Guo Boliang , Mulder Roger T , Kemp Samuel , Barrett Barbara 
Published: May 2017

ObjectiveTo investigate the cost-effectiveness of a modified form of cognitive behavioural therapy (CBT) for recurrent non-cardiac chest pain.MethodsWe tested the effectiveness and cost-effectiveness of a modified form of CBT for chest pain (CBT-CP)(4–10 sessions) in patients who attended cardiology clinics or emergency medical services repeatedly. Patients were randomised using a remote web-based system to CBT-CP or to standard care in the clinic. Assessments were made at baseline and at 6 months and 12 months. The primary outcome was the change in the Health Anxiety Inventory Score at 6 months. Other clinical measures, social functioning, quality of life and costs of services were also recorded.ResultsSixty-eight patients were randomised with low attrition rates at 6 months and 12 months with 81% of all possible assessments completed at 6 months and 12 months. Although there were no significant group differences between any of the outcome measures at either 6 months or 12 months, patients receiving CBT-CP had between two and three times fewer hospital bed days, outpatient appointments, and A&E attendances than those allocated to standard care and total costs per patient were £1496.49 lower, though the differences in costs were not significant. There was a small non-significant gain in quality adjusted life years in those allocated to CBT-CP compared with standard care (0.76 vs 0.74).ConclusionsIt is concluded that CBT-CP in the context of current hospital structures is not a viable treatment, but is worthy of further research as a potentially cost-effective treatment for non-cardiac chest pain.Trial registration numberISRCTN 14711101.
Effect of message congruency on attention and recall in pictorial health warning labels
Authors: Lochbuehler Kirsten , Mercincavage Melissa , Tang Kathy Z , Dana Tomlin C , Cappella Joseph N , Strasser Andrew A 
Published: May 2017

ObjectiveThe nine pictorial health warning labels (PWLs) proposed by the US Food and Drug Administration vary in format and feature of visual and textual information. Congruency is the degree to which visual and textual features reflect a common theme. This characteristic can affect attention and recall of label content. This study investigates the effect of congruency in PWLs on smoker’s attention and recall of label content.Methods120 daily smokers were randomly assigned to view either congruent or incongruent PWLs, while having their eye movements recorded. Participants were asked to recall label content immediately after exposure and 5 days later.ResultsOverall, the image was viewed more and recalled better than the text. Smokers in the incongruent condition spent more time focusing on the text than smokers in the congruent condition (p=0.03), but dwell time of the image did not differ. Despite lower dwell time on the text, smokers in the congruent condition were more likely to correctly recall it on day 1 (p=0.02) and the risk message of the PWLs on both day 1 (p=0.01) and day 5 (p=0.006) than smokers in the incongruent condition.ConclusionsThis study identifies an important design feature of PWLs and demonstrates objective differences in how smokers process PWLs. Our results suggest that message congruency between visual and textual information is beneficial to recall of label content. Moreover, images captured and held smokers’ attention better than the text.
Anode-supported BaZr0.8Y0.2O3−δ membranes by tape casting and suspension spraying
Authors: Xiao Jin , Yuan Hongchun , Chen Lei , Xiong Chao , Ma Jinxiang , Zhao Yu , Chai Jimin , Du Wenhan , Zhu Xifang 
Published: May 2017

Dense BaZr[Formula: see text]Y[Formula: see text]O[Formula: see text] (BZY) proton-conducting electrolyte membranes are successfully fabricated on NiO–BaZr[Formula: see text]Ce[Formula: see text]Y[Formula: see text]O[Formula: see text](NiO–BZCY) using tape casting combined with suspension spraying technique. The NiO–BZCY anode substrates were prepared by the tape-casting method and the BZY electrolyte membranes were prepared on the NiO-BZCY anodes by suspension spraying. The thermal decomposition of the anode green tape prepared by tape casting was investigated by thermogravimetry-differential thermal analysis while the phase structure and the morphology of the anode/electrolyte bi-layers were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). No obvious reaction was found between NiO and BZCY in anode substrates and gas-tight BZY membranes were achieved after co-sintering at 1450[Formula: see text]C. With Sm[Formula: see text]Sr[Formula: see text]CoO[Formula: see text]–Ce[Formula: see text]Sm[Formula: see text]O[Formula: see text](SSC–SDC) as the composite cathode, a maximum power density of 121 mW cm[Formula: see text] was obtained at 650[Formula: see text]C for the single cell with 23 [Formula: see text]m thick BZY electrolyte. Resistances of the tested cell were investigated under open circuit conditions at different operating temperatures by impedance spectroscopy.
Solution of certain Pell equations
Authors: Raza Zahid , Malik Hafsa Masood 
Published: May 2017

Let [Formula: see text] be any positive integers such that [Formula: see text] and [Formula: see text] is a square free positive integer of the form [Formula: see text] where [Formula: see text] and [Formula: see text] The main focus of this paper is to find the fundamental solution of the equation [Formula: see text] with the help of the continued fraction of [Formula: see text] We also obtain all the positive solutions of the equations [Formula: see text] and [Formula: see text] by means of the Fibonacci and Lucas sequences.Furthermore, in this work, we derive some algebraic relations on the Pell form [Formula: see text] including cycle, proper cycle, reduction and proper automorphism of it. We also determine the integer solutions of the Pell equation [Formula: see text] in terms of [Formula: see text] We extend all the results of the papers [3, 10, 27, 37].
Assessment of Breast Cancer Risk Factors Reveals Subtype Heterogeneity
Authors: Holm Johanna , Eriksson Louise , Ploner Alexander , Eriksson Mikael , Rantalainen Mattias , Li Jingmei , Hall Per , Czene Kamila 
Published: May 2017

Abstract
Subtype heterogeneity for breast cancer risk factors has been suspected, potentially reflecting etiologic differences and implicating risk prediction. However, reports are conflicting regarding the presence of heterogeneity for many exposures. To examine subtype heterogeneity across known breast cancer risk factors, we conducted a case–control analysis of 2,632 breast cancers and 15,945 controls in Sweden. Molecular subtype was predicted from pathology record–derived IHC markers by a classifier trained on PAM50 subtyping. Multinomial logistic regression estimated separate ORs for each subtype by the exposures parity, age at first birth, breastfeeding, menarche, hormone replacement therapy use, somatotype at age 18, benign breast disease, mammographic density, polygenic risk score, family history of breast cancer, and BRCA mutations. We found clear subtype heterogeneity for genetic factors and breastfeeding. Polygenic risk score was associated with all subtypes except for the basal-like (Pheterogeneity &lt; 0.0001). “Never breastfeeding” was associated with increased risk of basal-like subtype [OR 4.17; 95% confidence interval (CI) 1.89–9.21] compared with both nulliparity (reference) and breastfeeding. Breastfeeding was not associated with risk of HER2-overexpressing type, but protective for all other subtypes. The observed heterogeneity in risk of distinct breast cancer subtypes for germline variants supports heterogeneity in etiology and has implications for their use in risk prediction. The association between basal-like subtype and breastfeeding merits more research into potential causal mechanisms and confounders. Cancer Res; 77(13); 3708–17. ©2017 AACR.
Engineering and Functional Characterization of Fusion Genes Identifies Novel Oncogenic Drivers of Cancer
Authors: Lu Hengyu , Villafane Nicole , Dogruluk Turgut , Grzeskowiak Caitlin L. , Kong Kathleen , Tsang Yiu Huen , Zagorodna Oksana , Pantazi Angeliki , Yang Lixing , Neill Nicholas J. , Kim Young Won , Creighton Chad J. , Verhaak Roel G. , Mills Gordon B. , Park Peter J. , Kucherlapati Raju , Scott Kenneth L. 
Published: May 2017

Abstract
Oncogenic gene fusions drive many human cancers, but tools to more quickly unravel their functional contributions are needed. Here we describe methodology permitting fusion gene construction for functional evaluation. Using this strategy, we engineered the known fusion oncogenes, BCR-ABL1, EML4-ALK, and ETV6-NTRK3, as well as 20 previously uncharacterized fusion genes identified in The Cancer Genome Atlas datasets. In addition to confirming oncogenic activity of the known fusion oncogenes engineered by our construction strategy, we validated five novel fusion genes involving MET, NTRK2, and BRAF kinases that exhibited potent transforming activity and conferred sensitivity to FDA-approved kinase inhibitors. Our fusion construction strategy also enabled domain-function studies of BRAF fusion genes. Our results confirmed other reports that the transforming activity of BRAF fusions results from truncation-mediated loss of inhibitory domains within the N-terminus of the BRAF protein. BRAF mutations residing within this inhibitory region may provide a means for BRAF activation in cancer, therefore we leveraged the modular design of our fusion gene construction methodology to screen N-terminal domain mutations discovered in tumors that are wild-type at the BRAF mutation hotspot, V600. We identified an oncogenic mutation, F247L, whose expression robustly activated the MAPK pathway and sensitized cells to BRAF and MEK inhibitors. When applied broadly, these tools will facilitate rapid fusion gene construction for subsequent functional characterization and translation into personalized treatment strategies. Cancer Res; 77(13); 3502–12. ©2017 AACR.
Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response
Authors: Li Zhiguo , Li Jie , Kong Yifan , Yan Shan , Ahmad Nihal , Liu Xiaoqi 
Published: May 2017

Abstract
The mitotic kinase Plk1 contributes to the DNA damage response (DDR) by targeting multiple factors downstream of the core responder kinase ATM/ATR. In this study, we show that Polo-like kinase 1 (Plk1) also phosphorylates key factors upstream of ATM/ATR and regulates their DDR-related functions. Plk1 phosphorylated Mre11, a component of the Mre11/Rad50/Nbs1 (MRN) complex, at serine 649 (S649) during DDR. Phosphorylation of Mre11-S649 by Plk1 primed subsequent CK2-mediated phosphorylation at Mre11-serine 688 (S688). Phosphorylation of Mre11 at S649/S688 inhibited loading of the MRN complex to damaged DNA, leading to both premature DNA damage checkpoint termination and inhibition of DNA repair. Tumors expressing phosphomimetic Mre11 were more sensitive to the PARP inhibitor olaparib, compared with those expressing unphosphorylatable Mre11, suggesting that patients with elevated Plk1 expression might benefit from olaparib treatment. Cancer Res; 77(12); 3169–80. ©2017 AACR.