bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different Biomechanical Cell Behaviors in an Epithelium Drive Collective Epithelial Cell Extrusion
Authors: Balasubramaniam Lakshmi , Jain Shreyansh , Dang Tien , Lagoutte Emilie , Marc Mège René , Chavrier Philippe , Ladoux Benoit , Rossé Carine 
Published: September 2024

AbstractIn vertebrates, many organs, such as the kidney and the mammary gland form ductal structures based on the folding of epithelial sheets. The development of these organs relies on coordinated sorting of different cell lineages in both time and space, through mechanisms that remain largely unclear. Tissues are composed of several cell types with distinct biomechanical properties, particularly at cell‐cell and cell‐substrate boundaries. One hypothesis is that adjacent epithelial layers work in a coordinated manner to shape the tissue. Using in vitro experiments on model epithelial cells, differential expression of atypical Protein Kinase C iota (aPKCi), a key junctional polarity protein, is shown to reinforce cell epithelialization and trigger sorting by tuning cell mechanical properties at the tissue level. In a broader perspective, it is shown that in a heterogeneous epithelial monolayer, in which cell sorting occurs, forces arising from epithelial cell growth under confinement by surrounding cells with different biomechanical properties are sufficient to promote collective cell extrusion and generate emerging 3D organization related to spheroids and buds. Overall, this research sheds light on the role of aPKCi and the biomechanical interplay between distinct epithelial cell lineages in shaping tissue organization, providing insights into the understanding of tissue and organ development.
Ultralow Lattice Thermal Conductivity and Large Glass‐Like Contribution in Cs3Bi2I6Cl3: Rattling Atoms and p‐Band Electrons Driven Dynamic Rotation
Authors: Wu Yu , Ji Jialin , Ding Yimin , Yang Jiong , Zhou Liujiang 
Published: September 2024

AbstractUnderstanding the origin of ultralow lattice thermal conductivity κL of halide perovskites is of great significance in the energy conversion field. The soft phonon modes and the large anharmonicity corresponding to the dynamic rotation of halogen atoms play an important role in limiting the thermal transport of halide perovskites. The dynamic rotation has long been thought to originate from the electrostatic repulsion of lone pairs around halogen atoms. Here, by studying the layered perovskite Cs3Bi2I6Cl3, it is found that the interaction between the lone pairs contributed by the s bands of halogen atoms is short‐range, and the dynamic rotation is really driven by the occupied p‐band electrons. It dominates Cs3Bi2I6Cl3 with ultralow κL, < 0.2 W mK−1 at 300 K. Moreover, soft optical phonons are presented ≈1 and 2.2 THz that constitute relatively flat and dense bands due to the rattling Cs and Cl atoms, contributing a large glass‐like component to the κL. The results have important implications for understanding the origin of the ultralow κL in halide perovskites and for designing novel perovskites to serve the energy conversion field.
Coating Dormant Collagenase‐Producing Bacteria with Metal‐Anesthetic Networks for Precision Tumor Therapy
Authors: Han Qiuju , Yang Fengmin , Chen Mian , Zhang Mengmeng , Wang Lu , Wang Hongxia , Liu Jinyao , Cao Zhenping 
Published: September 2024

AbstractTumor malignancy highly depends on the stiffness of tumor matrix, which mainly consists of collagen. Despite the destruction of tumor matrix is conducive to tumor therapy, it causes the risk of tumor metastasis. Here, metal‐anesthetic network‐coated dormant collagenase‐producing Clostridium is constructed to simultaneously destruct tumor matrix and inhibit tumor metastasis. By metal‐phenolic complexation and π–π stacking interactions, a Fe3+‐propofol network is formed on bacterial surface. Coated dormant Clostridium can selectively germinate and rapidly proliferate in tumor sites due to the ability of carried Fe3+ ions to promote bacterial multiplication. Intratumoral colonization of Clostridium produces sufficient collagenases to degrade tumor collagen mesh and the loaded propofol restrains tumor metastasis by inhibiting tumor cell migration and invasion. Meanwhile, the delivered Fe3+ ions are reduced to the Fe2+ form by intracellular glutathione, thereby inducing potent Fenton reaction to trigger lipid peroxidation and ultimate ferroptosis of tumor cells. In addition to a satisfactory safety, a single intratumoral injection of coated dormant Clostridium not only effectively retards the growth of established large primary tumors, but also significantly suppresses distal lung metastasis in two different orthotopic tumor models. This work proposes a strategy to develop advanced therapeutics for malignant tumor treatment and metastasis prevention.
Metal‐Free Wet Chemistry for the Fast Gram‐Scale Synthesis of γ‐Graphyne and its Derivatives
Authors: Song Tao , Liu Hong , Zou Haiyuan , Wang Cheng , Shu Siyan , Dai Hao , Duan Lele 
Published: September 2024

Abstractγ‐Graphyne (GY), an emerging carbon allotrope, is envisioned to offer various alluring properties and broad applicability. While significant progress has been made in the synthesis of GY over recent decades, its widespread application hinges on developing efficient, scalable, and accessible synthetic methods for the production of GY and its derivatives. Here we report a facile metal‐free nucleophilic crosslinking method using wet chemistry for fast gram‐scale production of GY and its derivatives. This synthesis method involves the aromatic nucleophilic substitution reactions between fluoro‐(hetero)arenes and alkynyl silanes in the presence of a catalytic amount of tetrabutylammonium fluoride, where the fluoride plays a crucial role in removing protective groups from alkynyl silanes and generating reactive alkynylides. Our comprehensive analysis of the as‐prepared GY reveals a layered structure, characterized by the presence of the C(sp)−C(sp2) bond. The synthetic strategy shows remarkable tolerance to various functional groups and enables the preparation of diverse F‐/N‐rich GY derivatives, using electron‐deficient fluoro‐substituted (hetero)arenes as precursors. The feasibility of producing GY and derivatives from fluorinated (hetero)arenes through the metal‐free, scalable, and cost‐effective approach paves the way for broad applications of GY and may inspire the development of new carbon materials.
Dynamic Active Sites in Electrocatalysis
Authors: Ning Minghui , Wang Sangni , Wan Jun , Xi Zichao , Chen Qiao , Sun Yuanmiao , Li Hui , Ma Tianyi , Jin Huanyu 
Published: September 2024

AbstractIn‐depth understanding of the real‐time behaviors of active sites during electrocatalysis is essential for the advancement of sustainable energy conversion. Recently, the concept of dynamic active sites has been recognized as a potent approach for creating self‐adaptive electrocatalysts that can address a variety of electrocatalytic reactions, outperforming traditional electrocatalysts with static active sites. Nonetheless, the comprehension of the underlying principles that guide the engineering of dynamic active sites is presently insufficient. In this review, we systematically analyze the fundamentals of dynamic active sites for electrocatalysis and consider important future directions for this emerging field. We reveal that dynamic behaviors and reversibility are two crucial factors that influence electrocatalytic performance. By reviewing recent advances in dynamic active sites, we conclude that implementing dynamic electrocatalysis through variable reaction environments, correlating the model of dynamic evolution with catalytic properties, and developing localized and ultrafast in situ/operando techniques are keys to designing high‐performance dynamic electrocatalysts. This review paves the way to the development of the next‐generation electrocatalyst and the universal theory for both dynamic and static active sites.
Development of biochromic paper strips from cellulose nanocrystals and anthocyanin extract from pomegranate (Punica granatum L.) for colorimetric determination of urea
Authors: Al‐Qahtani Salhah D. , Attia Yasser A. , Al‐Senani Ghadah M. 
Published: September 2024

AbstractAn anthocyanin (ACN) spectroscopic probe was extracted and immobilized into a matrix of cellulose nanocrystals (CNC)/urease enzyme to create a colorimetric nanocomposite film sensor. The ACN@CNC composite is a disposable molecular biosensor that uses urease as a catalyst, ACN as a molecular probe, and CNC as a probe carrier with a high surface area. The ACN spectroscopic probe was isolated from the pomegranate peel (Punica granatum L.). A mordant was applied to fix ACN onto CNC by forming nanoparticles (6–14 nm) of mordant/ACN (M/ACN) complexation. CNC showed diameters in the range of 11–21 nm, and crystal lengths of 55–130 nm. Under acid/base conditions, the ACN probe solution in distilled water exhibited a reversible color change, as shown by the UV–Vis absorption spectra. In order to create a biocomposite film, CNC were reinforced with a sodium alginate biopolymer. Upon exposure to urea in an aqueous solution, the ACN@CNC film biosensor changes color from purple (598 nm) to white (432 nm). The detection limit of urea was determined at 25–450 ppm. Various methods were utilized to investigate the morphological properties of CNC and ACN@CNC films.
A study of crashworthiness performance in thin‐walled multi‐cell tubes 3D‐printed from different polymers
Authors: Tunay Merve , Bardakci Alperen 
Published: September 2024

AbstractMulticellular, thin‐walled impact tubes have been intensely studied and used in various engineering fields in recent years due to their lightweight, high performance, ease of application, superior energy absorption, and stable deformation characteristics. In this study, energy absorption, crashworthiness performances, and deformation properties of thin‐walled structures manufactured from polylactic acid (PLA+) and acrylonitrile butadiene styrene (ABS) using fused deposition modeling (FDM) technology were compared under quasi‐static axial compression. Thin‐walled structures consist of multicellular tubes connected by concentric corner‐edge connections with square and hexagonal cross‐sections. Experimental testing outcomes indicate that the energy absorption capacity increases with increasing the number of corners in multicellular structures. The tubes with square wall‐to‐wall (S‐WW) and hexagonal wall‐to‐wall (H‐WW) cross‐sections exhibit superior crashworthiness performance compared to other cross‐sections. Based on the experimental results, the absorbed energy by WW patterned PLA+ square tubes are 19%, 7%, and 46% more than that of wall‐to‐corner (WC), corner‐to‐wall (CW), and corner‐to‐corner (CC) patterned tubes, respectively, while it is 11%, 19%, and 80% more in hexagonal cross‐section tubes, respectively. This study provides an informative reference for easier applicability of multicellular energy‐absorbing structures with 3D‐print and the design of corner‐edge connections of internal connections in multicellular structures.
Improving the electromechanical deformability of MWCNT/silicone composites via encapsulating MWCNT with polyphenols and multilayered structure regulation
Authors: Sun Yue , Zhang Jiale , Chen Tao , Zhang Yanting , Wu Chonggang , Gong Xinghou , Hu Tao 
Published: September 2024

AbstractSilicone rubber (SR) is an ideal dielectric elastomer substrate due to its excellent flexibility and fast response speed. However, the innate low dielectric permittivity (ε) of SR generally requires a rather high driving voltage that restricts its widespread application. Typical attempts to increase ε of SR usually deteriorate either its flexibility or electrical stability. Herein, conductive multi‐walled carbon nanotube (MWCNT) were first surface modified with polyphenols (PNs) (MWCNT@PNs), aiming to facilitate its well dispersion within SR matrix, which may maintain the softness and electrical stability of SR via suppressing concentrated physical crosslinking and local leakage current flow. Then, five‐layered MWCNT@PNs/SR composites were prepared with the outer two insulating layers of SR while middle three dielectric layers of MWCNT@PNs filled SR. The multilayered structure further hindered the formation of conductive pathways through the composites, promising a high breakdown strength of the composites. Therefore, the multilayered MWCNT@PNs/SR composites exhibited increased ε, maintained low Young's modulus and electrical breakdown strength compared with pure SR of the same five‐layered structure. Among them, the composite with uniformly distributed MWCNT@PNs (m‐1: 1: 1) showed a highest actuation strain of 11.9% (at 19.6 kV mm−1), which was 4.1 times higher than that of SR (2.9% at 19.1 kV mm−1).
Development of new pyrazoles as class I HDAC inhibitors: Synthesis, molecular modeling, and biological characterization in leukemia cells
Authors: Berluti Francesco , Baselious Fady , Hagemann Sven , Hilscher Sebastian , Schmidt Matthias , Hüttelmaier Stefan , Schutkowski Mike , Sippl Wolfgang , Ibrahim Hany S. 
Published: September 2024

AbstractClass I histone deacetylases (HDACs) are considered promising targets in current cancer research. To obtain subtype‐selective and potent HDAC inhibitors, we used the aminobenzamide scaffold as the zinc‐binding group and prepared new derivatives with a pyrazole ring as the linking group. The synthesized compounds were analyzed in vitro using an enzymatic assay against HDAC1, −2, and −3. Compounds 12b, 15b, and 15i were found to be potent HDAC1 inhibitors, also in comparison to the reference compounds entinostat and tacedinaline, with IC50 values of 0.93, 0.22, and 0.68 μM, respectively. The best compounds were measured for their cellular effect and target engagement in acute myeloid leukemia (AML) cells. In addition, we studied the interaction of the compounds with HDAC subtypes using docking and molecular dynamic simulations. In summary, we have developed a new chemotype of HDAC1 inhibitors that can be used for further structure‐based optimization.
Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review
Authors: Goodarzi Maryam Moazami , Jalalirad Reza 
Published: September 2024

AbstractIncreasing attention has been paid to the purity of therapeutic proteins imposing extensive costs and challenges to the downstream processing of biopharmaceuticals. One of the efforts, that has been exerted to overcome such limitations, was developing multimodal or mixed‐mode chromatography (MMC) resins for launching selective, orthogonal, non‐affinity purification platforms. Despite relatively extensive usage of MMC resins, their real potential and fulfillment have not been extensively reviewed yet. In this work, the explanation of practical and key aspects of downstream processing of recombinant proteins with or without MMC resins was debated, as being useful for further purification process development. This review has been written as a step‐by‐step guide to deconvolute both inherent protein purification and MMC complexities. Here, after complete elucidation of the potential of MMC resins, the effects of frequently used additives (mobile phase modifiers) and their possible interactions during the purification process, the critical characteristics of common product‐related impurities (e.g., aggregates, charge variants, fragments), host‐related impurities (e.g., host cell protein and DNA) and process related impurities (e.g., endotoxin, and viruses) with solved or unsolved challenges of traditional and MMC resins have been discussed. Such collective experiences which are reported in this study could be considered as an applied guide for developing successful downstream processing in challenging conditions by providing a clear insight into complex MMC resins and impurities.
3D bioprinting technology and equipment based on microvalve control
Authors: Kang Rihui , Wu Jiaxing , Cheng Rong , Li Meng , Sang Luxiao , Zhang Hulin , Sang Shengbo 
Published: September 2024

Abstract3D bioprinting technology is widely used in biomedical fields such as tissue regeneration and constructing pathological model. The prevailing printing technique is extrusion‐based bioprinting. In this printing method, the bioink needs to meet both printability and functionality, which are often conflicting requirements. Therefore, this study has developed an innovative microvalve‐based equipment, incorporating components such as pressure control, a three‐dimensional motion platform, and microvalve. Here, we present a droplet‐based method for constructing complex three‐dimensional structures. By leveraging the rapid switching characteristics of the microvalve, this equipment can achieve precise printing of bio‐materials with viscosities as low as 10mPa·s, significantly expanding the biofabrication window for bioinks. This technology is of great significance for 3D bioprinting in tissue engineering and lays a solid foundation for the construction of complex artificial organ tissues.
Discrepancies between guidelines, preferences and actual activities in psychotherapy supervision
Authors: Spännargård Åsa , Fagernäs Simon , Alfonsson Sven 
Published: September 2024

AbstractBackgroundClinical supervision, also known as consultation, is a key component of professional growth for psychotherapists, often involving activities like case discussions or practical exercises. However, the extent to which the content of supervision follows practice guidelines and whether supervision meets the preferences of psychotherapists is largely unclear.AimsThis study aimed to understand what psychotherapists prefer to do in clinical supervision and contrast that to what their supervision comprises. A secondary aim was to explore whether there were any differences in preferred activities between psychotherapists with different background variables, such as profession and training, and to examine associations between activities, supervision satisfaction and effectiveness.Materials & MethodsPsychotherapists (N = 243, 84% women, mean age 46.3 years, SD = 10.6) completed an online survey regarding their supervision preferences and actual supervision activities.ResultsThe psychotherapists reported that formative activities, such as concrete exercises, discussing recorded material and modelling, were significantly more preferred than they were occurring in clinical supervision. The activities of teaching, modelling and discussions on emotional reactions, which are more associated with restoration, were associated with supervision satisfaction and competence. Psychotherapists with a cognitive behavioural therapy orientation preferred more active learning activities than other psychotherapists.ConclusionsOverall, neither the preferred nor the actual activities aligned with existing guidelines for supervision practices. Still, the therapists reported a preference for more experiential learning activities rather than more verbal activities.
Accounting for informalization in Africa's extractive industry: Evidence from Ghana's small‐scale gold mining sector during the colonial period
Authors: Tuffour James , Patnaik Swetketu , Ayikoru Maureen 
Published: September 2024

AbstractIn this paper, we draw on the accountability and institutional theories to explicate the informalized state of Ghana's small‐scale gold mining sector as an exemplar of informalization of the extractive industry in Africa during the colonial period. We present a critical and historical perspective on the role of colonial subjugation that took the form of discriminatory policies and lack of accountability, in laying the foundations for the current challenges of continuous informalization of the indigenous gold mining sector and its implications on livelihoods, the economy and the environment in Ghana. We analyzed archival data on the mining industry in Ghana, consisting of official correspondences between colonial bureaucrats in Britain and Ghana, as well as colonial policies on mining, interpreted through the lenses of institutional theory. Specifically, we used records comprising letters, mineral policies, mineral output, and labor relations in the Gold Coast during the colonial period as well as minutes of meetings held by those governing mining enterprises that operated in Ghana before 1957. We also drew on periodic reports of the Ghana Chamber of Mines and their member enterprises, Government of Ghana white papers, parliamentary records among others. We found that the colonial authority, as the new institutional power with vested interest in the extractive industry, adopted discriminatory policies that banned small‐scale gold mining by indigenous population in Ghana, marginalizing the sector's operation to the advantage of foreign large‐scale gold miners. We demonstrate that the control of mineral resources by the colonial authority under new ownership structures disenfranchised the indigenous population and their right to the mineral resources of the Gold Coast. We argue that this institutionalized discriminatory policy, and the resulting lack of accountability is largely responsible for the ongoing informalization of Ghana's small‐scale gold mining sector. This paper contributes to the corpus of critical and historical scholarship that investigates the role of racialization and accountability during colonial period, but with notable distinction that departs from specific professional or disciplinary perspectives. Instead, it focuses on the colonial subjugation and exploitation of the small‐scale gold mining sector and its ongoing informalization that traces its roots to the era of British colonial rule in Ghana. It reiterates the need for accountability for these historical inequalities affecting the small‐scale mining operators, to facilitate the sector's formalization and to effectively control and manage the myriads of negative impacts associated with it.
Pulmonary complications post allogeneic haematopoietic stem cell transplant in children
Authors: Walker Hannah , Abbotsford Joanne , Haeusler Gabrielle M , Yeoh Daniel , Ramachandran Shanti , Ng Michelle , Holzmann Jonathan , Shanthikumar Shivanthan , Weerdenburg Heather , Hanna Diane , Neeland Melanie R , Cole Theresa 
Published: September 2024

AbstractObjectivesHaematopoietic stem cell transplant (HCT) is a cellular therapy that, whilst curative for a child's underlying disease, carries significant risk of mortality, including because of pulmonary complications. The aims of this study were to describe the burden of pulmonary complications post‐HCT in a cohort of Australian children and identify risk factors for the development of these complications.MethodsPatients were identified from the HCT databases at two paediatric transplant centres in Australia. Medical records were reviewed, and demographics, HCT characteristics and pulmonary complications documented. Relative risk ratio was used to identify risk factors for developing pulmonary complications prior to first transplant episode, and survival analysis performed to determine hazard ratio.ResultsIn total, 243 children underwent transplant during the study period, and pulmonary complications occurred in 48% (117/243) of children. Infectious complications were more common (55%) than non‐infective complications (18%) and 26% of patients developed both. Risk factors for the development of pulmonary complications included the following: diagnoses of MPAL (RR 2.16, P = 0.02), matched unrelated donor (RR1.34, P = 0.03), peripheral blood (RR 1.36, P = 0.028) or cord blood (RR 1.73, P = 0.012) as the stem cell source and pre‐existing lung disease (RR1.72, P < 0.0001). Children with a post‐HCT lung complication had a significantly increased risk of mortality compared with those who did not (HR 3.9, P < 0.0001).ConclusionThis study demonstrates pulmonary complications continue to occur frequently in children post‐HCT and contribute significantly to mortality. Highlighting the need for improved strategies to identify patients at risk pre‐transplant and enhanced treatments for those who develop lung disease.