bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between cardiometabolic index and congestive heart failure among US adults: a cross-sectional study
Authors: Luo Xi , Cai Bin 
Published: September 2024

BackgroundThe risk of congestive heart failure (CHF) is significantly affected by obesity. However, data on the association between visceral obesity and the risk of CHF remain limited. We explored the relationship between CHF and cardiometabolic index (CMI).MethodsDrawing from the National Health and Nutrition Examination Survey (NHANES) for 2011–2018, we enrolled 9,008 participants in a cross-sectional study. We calculated the CMI as triglyceride (TG)/high density lipid-cholesterol (HDL-C) × weight-to-height ratio (WHtR), and CMI-age as CMI × age. Then, we analyzed CMI and CMI-age as categorical and continuous variables to assess its correlation with CHF. To assess the relationships of CMI and CMI-age with CHF, we used multiple logistic regression models and performed subgroup analysis. To examine the predictive ability of CMI and CMI-age on patients with CHF, we used receiver operating characteristic (ROC) curves.ResultsThe overall prevalence of CHF was 3.31%. The results revealed significant differences in demographic data, comorbidities, lifestyle variables, standing height, BMI, WC, WHtR, TG, and HDL-C among the four groups classified by CMI quartile and CMI-age quartile. When indicators were analyzed as continuous variables, CMI and CMI-age showed positive correlations with CHF in both the crude and adjusted models (all P &lt; 0.05). When indicators were analyzed as categorical variables, it was found that in all four models, the ORs of group Q4 was significantly different compared to Q1 (all P &lt; 0.05), suggesting the risk of CHF is significantly increased with higher CMI, and CMI-age. The associations of CMI and CMI-age with CHF were similar in all stratified populations (P for interaction &gt; 0.05). The areas under the ROC curve (AUCs) of CMI and CMI-age in predicting CHF were 0.610 (95% CI, 0.578–0.642) and 0.697 (95% CI, 0.668–0.725) separately, suggesting that CMI-age was significantly better than the CMI in predicting CHF (P &lt; 0.001).ConclusionsBoth CMI and CMI-age were independently correlated with the risk for CHF. These results suggested that the CMI-age, which provides new insights into the prevention and management of CHF. CMI-age could serve as effective tools to identify CHF during primary care examinations and in medically resource-limited areas.
Do NSAIDs affect bone healing rate, delay union, or cause non-union: an updated systematic review and meta-analysis
Authors: Chuang Po-Yao , Yang Tien-Yu , Tsai Yao-Hung , Huang Kuo-Chin 
Published: September 2024

IntroductionNonsteroidal anti-inflammatory drugs (NSAIDs) may potentially delay or cause non-union of fractures by inhibiting prostaglandin synthesis. However, studies have shown conflicting results. This systematic review and meta-analysis aim to synthesize current evidence on the potential influence of NSAIDs on bone healing.MethodsWe conducted a comprehensive search of PubMed, Embase, and Cochrane CENTRAL databases for studies published up to 25 July 2023. Specific keywords included “NSAID,” “nonsteroidal anti-inflammatory drug,” “cyclooxygenase-2 inhibitor,” “bone healing,” “non-union,” “pseudoarthrosis,” “delayed union,” and “atrophic bone.” Eligible studies included prospective, retrospective, and case-controlled studies assessing the correlation between NSAID use and bone healing outcomes. The leave-one-out approach was used to test the robustness of the meta-analysis results.ResultsA total of 20 studies with 523,240 patients were included in the analysis. The mean patient age ranged from 6.7 to 77.0 years, with follow-up durations from 3 to 67 months. The meta-analysis revealed no significant difference in non-union or delayed union between NSAID users and non-users [pooled adjusted odds ratio (OR) = 1.11; 95% confidence interval (CI): 0.99–1.23]. Initial analysis identified a significant association between NSAID usage and an increased risk of reoperation, but this association became insignificant upon sensitivity analysis (crude OR = 1.42; 95% CI: 0.88–2.28).DiscussionNSAIDs may have a minimal impact on non-union or delayed union risks. However, caution is advised due to the limited number of studies and the absence of a specific focus on NSAID types and dosages. Further research is necessary to better understand the implications of NSAID use on bone healing.
Identification of two heterogeneous subtypes of hepatocellular carcinoma with distinct pathway activities and clinical outcomes based on gene set variation analysis
Authors: Jin Zhipeng , Wang Xin , Zhang Xue , Cheng Siqi , Liu Yefu 
Published: September 2024

BackgroundHigh heterogeneity is an essential feature of malignant tumors. This study aims to reveal the drivers of hepatocellular carcinoma heterogeneity for prognostic stratification and to guide individualized treatment.MethodsOmics data and clinical data for two HCC cohorts were derived from the Cancer Genome Atlas (TCGA) and the International Cancer Genome Atlas (ICGC), respectively. CNV data and methylation data were downloaded from the GSCA database. GSVA was used to estimate the transcriptional activity of KEGG pathways, and consensus clustering was used to categorize the HCC samples. The pRRophetic package was used to predict the sensitivity of samples to anticancer drugs. TIMER, MCPcounter, quanTIseq, and TIDE algorithms were used to assess the components of TME. LASSO and COX analyses were used to establish a prognostic gene signature. The biological role played by genes in HCC cells was confirmed by in vitro experiments.ResultsWe classified HCC tissues into two categories based on the activity of prognostic pathways. Among them, the transcriptional profile of cluster A HCC is similar to that of normal tissue, dominated by cancer-suppressive metabolic pathways, and has a better prognosis. In contrast, cluster B HCC is dominated by high proliferative activity and has significant genetic heterogeneity. Meanwhile, cluster B HCC is often poorly differentiated, has a high rate of serum AFP positivity, is prone to microvascular invasion, and has shorter overall survival. In addition, we found that mutations, copy number variations, and aberrant methylation were also crucial drivers of the differences in heterogeneity between the two HCC subtypes. Meanwhile, the TME of the two HCC subtypes is also significantly different, which offers the possibility of precision immunotherapy for HCC patients. Finally, based on the prognostic value of molecular subtypes, we developed a gene signature that could accurately predict patients’ OS. The riskscore quantified by the signature could evaluate the heterogeneity of HCC and guide clinical treatment. Finally, we confirmed through in vitro experiments that RFPL4B could promote the progression of Huh7 cells.ConclusionThe molecular subtypes we identified effectively exposed the heterogeneity of HCC, which is important for discovering new effective therapeutic targets.
Characteristics and real-world medication persistence of people living with HIV treated with DTG/3TC or BIC/FTC/TAF: a hospital claims database study in Japan
Authors: Kanamori Rie , Aoki Nozomi , Kanazawa Akio , Yuda Mayumi , Makino Nao , Ohata Emi , Fukui Nobuyuki , Mori Hirotake , Yokokawa Hirohide , Naito Toshio 
Published: September 2024

BackgroundAs the life expectancy of people living with human immunodeficiency virus (HIV) (PLWH) has improved, chronic disease burden and polypharmacy have increased in PLWH. Simplification of the antiretroviral therapy (ART) regimen for PLWH has become crucial. The real-world treatment patterns and medication persistence of the 2-drug single-tablet regimen (STR), dolutegravir/lamivudine (DTG/3TC), compared to bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) prescribed were investigated.MethodsThis retrospective, database study extracted data from a hospital-based medical claims database in Japan. The changes in ART distributions by year during the identification period between January 1, 2018 and December 31, 2021 were observed. Patients with disease record of HIV-1 infection and prescribed DTG/3TC or BIC/FTC/TAF as the first prescription of STR during the identification period were divided into two cohorts; DTG/3TC cohort and BIC/FTC/TAF cohort, respectively. Patient without medication records more than 3 months and no future data more than 6 months were excluded. Patients’ characteristics were compared between the DTG/3TC cohort and the BIC/FTC/TAF cohort by Mantel–Haenszel test to adjust for age. Medication persistence was compared between the two cohorts by evaluating the continuation rates using Kaplan–Meier methods, using the log-rank test to assess the difference between the Kaplan–Meier curves. The median time-to-first prescription was compared between the two cohorts by Kaplan–Meier methods.ResultsPrescriptions of DTG/3TC and BIC/FTC/TAF increased steadily from 2019 to 2021 after the release year of each STR. There was no significant difference in the time-to-first prescription (p = 0.3). A total of 959 patients were included, with 120 patients and 839 patients on DTG/3TC and BIC/FTC/TAF, respectively. The proportion of dyslipidemia at baseline was significantly higher in the DTG/3TC cohort than in the BIC/FTC/TAF cohort after adjusting for mean age (p = 0.002). There was no significant difference in medication persistence between the two cohorts (p = 0.91).ConclusionThis study showed that DTG/3TC was likely to be selected for elderly patients and those with chronic disease in real-world clinical practice, which seems in accordance with the treatment strategy recommended by guidelines. Comparable medication persistence was observed with both regimens, aligning with findings from other countries. The 2-drug single-tablet regimen DTG/3TC may be an important ART regimen for PLWH with multiple morbidities and polypharmacy in an aging society. Due to the limitations of the database, further research to assess viral loads, emergence of resistance and adverse events will be encouraged.
Investigating the molecular mechanisms of Fuzheng Yiliu Shenji prescription in SH-SY5Y neuroblastoma cells
Authors: Zhu Xueying , Si Yinchu , Gai Cong , Li Zhong 
Published: September 2024

BackgroundNeuroblastoma is the most common extracranial solid tumor in childhood. Fuzheng Yiliu Shenji Prescription (FYSP) has shown potential in treating malignant pediatric tumors in clinical settings. This study aims to explore the molecular mechanisms behind its effects, specifically in the context of neuroblastoma cell lines.ObjectiveTo elucidate the active compounds in FYSP and their mechanisms of action in inhibiting neuroblastoma cell viability, inducing apoptosis, and affecting the cell cycle in SH-SY5Y cells through network pharmacology and empirical validation.Materials and methodsWe identified the major compounds in FYSP and their predicted targets, constructing a protein-protein interaction (PPI) network and performing GO and KEGG pathway analyses. The effects of FYSP were empirically validated through assays on cell viability, cell cycle, apoptosis, and protein expression in SH-SY5Y cells.ResultsThe study identified 172 active chemical components in FYSP, with 188 common targets related to neuroblastoma. Network analysis highlighted the PI3K-Akt pathway as a significant target. Experimental validation in SH-SY5Y cells confirmed that FYSP could inhibit cell viability, induce G2/M cell cycle arrest, and promote apoptosis through modulation of the PI3K-Akt pathway, specifically upregulating caspase-3 and downregulating Bcl-2/Bax expression.ConclusionThe study elucidates the molecular basis of FYSP’s effects on neuroblastoma cells in vitro, demonstrating its ability to modulate key pathways involved in cell cycle and apoptosis. While these findings suggest a potential therapeutic role for FYSP, they are limited to in vitro observations, and further research, including in vivo studies, is necessary to explore its clinical applicability.
Discovery of key biomarkers in tourette syndrome by network pharmacology
Authors: Zhao Jiali , Bai Xiaohong 
Published: September 2024

BackgroundYangxue Xifeng Decoction (YXD) has been utilized in clinical settings for the treatment of Tourette Syndrome (TS). However, the action mechanism of YXD needs further research.MethodsThe ingredients and targets of YXD were identified via database searches and then constructed an active ingredient-target network using Cytoscape. Pathway enrichment analysis was performed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The core genes were determined by LASSO regression and SVM algorithm. Additionally, we analyzed the immune infiltration. The signaling pathways associated with core genes were investigated through KEGG and GO. We predicted the transcription factors using “RcisTarge”.Results127 active ingredients of YXD and 255 targets were obtained. TNF and the IL-17 signaling pathway were the main pathways. OPRM1 and VIM were screened out as core genes, which were associated with the immune infiltration. The signaling pathways involved in OPRM1 and VIM were enriched. Furthermore, remarkable correlation was found between OPRM1 and VIM levels and other TS-related genes such as MAPT and MAPT.ConclusionOPRM1 and MAPT, and the signaling pathways are associated with TS. YXD exerts its therapeutic TS through multi-component and multi-targets including immune infiltration.
Revealing genetic causality between blood-based biomarkers and major depression in east Asian ancestry
Authors: Mo Xiaoxiao , Wang Chao , Pu Qiuyi , Zhang Zhengdong , Wu Dongmei 
Published: September 2024

IntroductionMajor Depression (MD) is a common mental disorder. In East Asian ancestry, the association, causality, and shared genetic basis between blood-based biomarkers and MD remain unclear.MethodsWe investigated the relationships between blood-based biomarkers and MD through a cross-sectional study and Mendelian randomization (MR) analysis. Cross-trait analysis and enrichment analyses were used to highlight the shared genetic determinants and biological pathways. We conducted summary data–based MR to identify shared genes, which were then validated using a transcriptome dataset from drug-naïve patients with MD.ResultsIn the cross-sectional study, C-Reactive Protein showed the significantly positive correlation with depressive symptoms, while hematocrit, hemoglobin, and uric acid exhibited significantly negative correlations. In MR analysis, basophil count (BASO) and low-density lipoprotein cholesterol (LDLc) had a significant causal effect on MD. The enrichment analysis indicated a significant role of inflammatory cytokines and oxidative stress. The shared genes MFN2, FAM55C, GCC2, and SCAPER were validated, with MFN2 identified as a pleiotropic gene involved in MD, BASO, and LDLc.DiscussionThis study highlighted that BASO and LDLc have a causal effect on MD in East Asian ancestry. The pathological mechanisms of MD are related not only to inflammatory cytokines and oxidative stress but also to down regulation of MFN2 expression and mitochondrial dysfunction.
Evaluation of a pre-post quasi-experimental educational intervention on breast cancer awareness among pharmacy professionals in Karachi, Pakistan
Authors: Mansoor Noor us Saba , Naveed Safila , Ali Huma , Manzoor Ammara , Zahoor Saima , Sheikh Javeria 
Published: September 2024

IntroductionCancer, particularly breast cancer, is a major contributor to mortality and a significant impediment to life expectancy. In 2020, breast cancer accounted for 11.7% of all cancer cases and caused approximately 685,000 deaths worldwide, surpassing lung cancer in prevalence. The study aims to evaluate the impact of an educational intervention on breast cancer awareness among pharmacy students by comparing their understanding before and after the program.MethodA pre-post quasi-experimental study was designed to assess knowledge and awareness of breast cancer, breast self-examination (BSE) practices, and attitudes toward breast cancer among female university students in Karachi, Pakistan. Participants completed a pre-session questionnaire, attended an awareness workshop and video tutorial, and then completed a post-session questionnaire 2 weeks later.ResultsOf 1,200 participants, 1,015 of them completed both the pre- and post-intervention questionnaires. Key demographic features included 83.9% of the participants being in the 18–24 age group, 26.8% being married, and only 14.2% having a family history of breast cancer. Before the intervention, 60.7% of the participants were not involved in regular breast self-exams due to a lack of awareness. Post-intervention results showed a significant increase in awareness, with 35.9% rising to 94.9%. The use of screening methods increased from 46.7 to 94.8%. Knowledge of breast cancer improved from 51.2 to 96.7%, and the general perception rose from 48.2 to 93.4%. Attitudes toward self-examination also shifted positively, indicating a significant change in perception.Interpretation and conclusionThe study concludes the baseline knowledge of breast cancer among female students was inadequate but improved significantly from over 40% to over 90% following the educational intervention. The intervention positively influenced the general perception and attitudes toward breast cancer. These findings highlight the need for regular educational sessions to enhance awareness, improve healthcare outcomes, and reduce mortality rates, particularly in developing countries.
Omnidirectional soft pneumatic actuators: a design and optimization framework
Authors: Moutousi Maria , Polygerinos Panagiotis 
Published: September 2024

IntroductionSoft pneumatic actuators (SPAs) play a pivotal role in soft robotics due to their unique characteristics of compliance, flexibility, and adaptability. There are plenty of approaches that examine the modeling parameters of SPAs, aiming to optimize their design and, thus, achieve the most advantageous responses. Current optimization methods applied to SPAs are usually performed individually for each design parameter without considering the simultaneous effect all parameters can have on the output performance. This modeling shortcoming is essential to be addressed since customized SPAs are used in a variety of applications, each with different output requirements.MethodsThis study provides a generalized design optimization framework for modeling the SPA performance for their motion profiles, the produced strain energy while being deformed, and their stiffness characteristics. Utilizing experimentally validated finite element methods, all geometrical and material parameters of the models are investigated in response surface methodology optimization using the central composite design approach.ResultsThe results showcase the entire design space of omnidirectional SPAs, along with their output performance, providing guidelines to the end user for design optimization.DiscussionThe offering of this modeling process for SPAs can be adapted to the demands of any potential application and ensure the best performance with respect to the required output responses.
Compreensão sobre o tratamento cirúrgico da endocardite em válvulas pulmonares Transcateter Melody
Authors: Gomes João Vitor Ferraz , Lopes Thayanne Mayara de Oliveira , Schiattarella Millan Christinne da Silva , Apolinário Marcos Vinicius Vieira , Brito Maria Eduarda Silva , Queiroz Lucas Ferraz Freire de , Cavalcanti Neto Eduardo Jorge de Souza , Soares Luiz Eduardo Vilar , Nepomuceno Tiago Leal , Torres Marcelo Florêncio , Marques David Arruda Tavares de Queiroz , Araújo Kaiky Gouveia Nóbrega Campelo , Reis Marcelo Arteiro Costa , Pragana Maria Luisa Sampaio de França , Cavalcante Nícolas Medeiros , Silva Lillian Jenne de Assis , Souza José Lucas Silva de , Texeira Thais Moura , Costa Walter Werleys Souza , Azevedo Renata Cristina Santos de , Perazzo Ana Clara Siqueira Teixeira , Marafante Maria Eduarda Osório 
Published: September 2024

Levando em consideração o aumento da dependência da substituição da válvula pulmonar transcateter (TPV) como uma alternativa minimamente invasiva para a o mal funcionamento da saída do ventrículo direito (TSVD), a válvula transcateter Melody tornou-se um exemplo no tratamento de condições cardíacas. Apesar da sua utilização ser disseminada e seus benefícios bastante significativos na redução da necessidade de cirurgias cardíacas abertas consecutivas, surgiram novas preocupações sobre complicações como a endocardite infecciosa e a trombose valvar,devido isso é necessário um manejo mais cuidadoso a esses pacientes. Este estudo revisa a literatura de forma sistemática sobre as complicações existentes, com foco na incidência e manejo da endocardite infecciosa e a durabilidade da válvula. Os achados indicam que, embora a válvula Melody seja eficaz na reabilitação da função da válvula pulmonar, os riscos de complicações, principalmente da endocardite infecciosa, são maiores em comparação com outras TPVs, a exemplo a válvula Sapien. O que nos mostra a importância do monitoramento contínuo e da criação de novas abordagens mais cuidadosas para o manejo desses pacientes. Diante disso, embora a válvula Melody seja valiosa para o manejo da disfunção do TSVD, mais pesquisas são necessárias para melhorar os resultados dos pacientes e reduzir as complicações mais graves, com foco na identificação de fatores desencadeantes precocemente e no desenvolvimento de protocolos de manejo eficazes.
PENGARUH SOSIAL MEDIA MARKETING, E-SERVICE QUALITY, DAN REVIEW CONTENT TERHADAP MINAT BELI KONSUMEN BOX OUTLET SINGARAJA
Authors: KUSUMA EKA , DEWI KOMANG AYU INTAN 
Published: September 2024

This study aims to examine the influence of Social Media Marketing, E-Service Quality, and Review Content on consumer buying interest in Singaraja Box Outlet Stores. The population in this study includes all Singaraja Box Outlet Store consumers from various walks of life, both men and women. The sample used in this study was 85 consumers selected through a questionnaire technique distributed using Google Form. This study used a quantitative approach and the data was analyzed by multiple linear regression method using IBM SPSS Statistics 25 application. The results showed that Social Media Marketing, E-Service Quality and Review Content proved to have a positive and significant influence on consumer buying interest in Singaraja Box Outlet Stores
Cuidados paliativos en oncología pediátrica: rol y desafíos de la enfermería
Authors: Villacreses-Merino Katherine Monserrate , Merino-Conforme Monserrate Cristina , Alcázar-Marcillo Angelica Adriana , Cantos-Sánchez María Monserrate 
Published: September 2024

Objetivo: Analizar los cuidados paliativos en oncología pediátrica: Rol y desafíos de la enfermería. Método: Se desarrolló desde un enfoque cuantitativo con una metodología descriptiva con diseño no experimental, la cual se apoyó en el análisis documental–bibliográfico. Se fundamentó en la exploración metódica, rigurosa y profunda de material documental de trabajos científicos. Resultados: La enfermería, como profesión, brinda cuidados mediante acciones e intervenciones basadas en fundamentos científicos y destrezas. Conclusión: La enfermería es parte esencial del equipo multidisciplinario de cuidados paliativos pediátricos, que requiere estar en constante actualización de conocimientos y su accionar bajo el sustento científico, con intervenciones farmacológicas y no farmacológicas direccionadas al uso de herramientas lúdicas y complementarias