bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dog Breed and Starch Gelatinisation Correlation in Food Digestibility and Faecal Traits
Authors: Pescuma Mariana Gilbert , Ximenes Thais Oliveira , Garcia Caroline Alves , de Ramos Eloise Cristina , de Oliveira Carolina Cristina , Henríquez Lucas Ben Fiuza , de Souza Theodoro Stephanie , Loureiro Bruna Agy , Carciofi Aulus Cavalieri 
Published: October 2024

ABSTRACTLarge dog breeds commonly produce unformed faeces. The present study hypothesised that foods for large dog breeds require higher starch gelatinisation (SG) to reduce organic matter flow to colon. Fifteen Rottweilers (Ro; 49.4 ± 6.12 kg), 18 Beagles (Be; 12.13 ± 1.75 kg) and 20 Shih‐Tzus (ST; 4.62 ± 1.15 kg) were fed one of three diets, all based on the same sorghum formulation, processed to obtain three levels of SG: SG90 (91.8% SG), SG50 (50.7% SG) and SG30 (27.4% SG). Foods were provided for 23 days, and the coefficient of total tract apparent digestibility (CTTAD) of nutrients, faecal production and fermentation products, Na, K and Cl apparent absorption and gastrointestinal transit time (GTT) were evaluated. Results were submitted to analysis of variance considering SG, breed and their interactions, and means compared by the Tukey test (p < 0.05). All dogs promptly ate the foods. Faeces scores were lower for Ro than for Be and ST, and lower for SG30 than SG90 (p < 0.05). Faeces pH was higher, and total short‐chain fatty acids were lower for SG90 than for SG50 and SG30 (p < 0.01), regardless of breed. No diet effect was observed for GTT (p > 0.05), but it was lower for Ro (41.7 ± 6.2 h) than for ST (48.7 ± 8.6 h). The CTTAD of nutrients was lower for SG30, intermediary for SG50 and higher for SG90 (p < 0.05), and among breeds CTTAD was higher for Be than ST and Ro (p < 0.05), which did not differ from each other, except for crude protein CTTAD which was lower for Ro (p < 0.05). The apparent absorption of Na and K was higher for the SG90 treatment, and for Be in comparison with Ro and ST (p < 0.05). Food SG had a remarkable influence on Ro faeces formation and on the CTTAD in all breeds. Greater fermentation in the colon and lower protein CTTAD may be involved in Ro unformed faeces formation.
Bigger genomes provide environment‐dependent growth benefits in grasses
Authors: Simpson Kimberley J. , Mian Sahr , Forrestel Elisabeth J. , Hackel Jan , Morton Joseph A. , Leitch Andrew R. , Leitch Ilia J. 
Published: October 2024

Summary

Increasing genome size (GS) has been associated with slower rates of DNA replication and greater cellular nitrogen (N) and phosphorus demands. Despite most plant species having small genomes, the existence of larger GS species suggests that such costs may be negligible or represent benefits under certain conditions.

Focussing on the widespread and diverse grass family (Poaceae), we used data on species' climatic niches and growth rates under different environmental conditions to test for growth costs or benefits associated with GS. The influence of photosynthetic pathway, life history and evolutionary history on grass GS was also explored.

We found that evolutionary history, photosynthetic pathway and life history all influence the distribution of grass species' GS. Genomes were smaller in annual and C4 species, the latter allowing for small cells necessary for C4 leaf anatomy. We found larger GS were associated with high N availability and, for perennial species, low growth‐season temperature.

Our findings reveal that GS is a globally important predictor of grass performance dependent on environmental conditions. The benefits for species with larger GS are likely due to associated larger cell sizes, allowing rapid biomass production where soil fertility meets N demands and/or when growth occurs via temperature‐independent cell expansion.
Alignment, Anticipation, Adaptation, or Lagging Behind? Age‐Based Regulations in Assisted Reproduction and Late Fertility
Authors: Compans Marie‐Caroline 
Published: October 2024

AbstractThis paper focuses on age restrictions on access to infertility treatments and eligibility for their public reimbursement, exploring their relevancy in contexts of rising late birth rates (40+). I explore how age‐based reimbursement policies for in vitro fertilization treatments have responded to these fertility trends in 27 high‐income countries and in which regulatory frameworks for medically assisted reproduction (MAR) very late births (45+) have particularly increased. First, I show that while age limits for treatment reimbursement are well aligned with the prevalence of late fertility in some national contexts, in most countries, strict age restrictions are lagging behind the rise in late births. In others, pronatalist policies have prompted permissive age criteria or law revisions, anticipating or adapting to rising trends in late births. Second, the rise in very late births has been limited in some contexts with strict age‐based rules. However, the analysis suggests that the impact of MAR on very late births may also be influenced by contextual factors other than regulations.
Stable Isotope Labelling Reveals Water and Carbon Fluxes in Temperate Tree Saplings Before Budbreak
Authors: Walde Manuel G. , Lehmann Marco M. , Gessler Arthur , Vitasse Yann , Diao Haoyu 
Published: October 2024

ABSTRACTDespite considerable experimental effort, the physiological mechanisms governing temperate tree species' water and carbon dynamics before the onset of the growing period remain poorly understood. We applied 2H‐enriched water during winter dormancy to the soil of four potted European tree species. After 8 weeks of chilling, hydrogen isotopes in stem, twig and bud water were measured six times during 2 consecutive weeks of forcing conditions (Experiment 1). Additionally, we pulse‐labelled above‐ground plant tissues using 2H‐enriched water vapour and 13C‐enriched CO2 7 days after exposure to forcing conditions to trace atmospheric water and carbon uptake (Experiment 2). Experiment 1 revealed soil water incorporation into the above‐ground organs of all species during the chilling phase and significant species‐specific differences in water allocation during the forcing conditions, which we attributed to differences in structural traits. Experiment 2 illustrated water vapour incorporation into all above‐ground tissue of all species. However, the incorporation of carbon was found for evergreen saplings only. Our results suggest that temperate trees take up and reallocate soil water and absorb atmospheric water to maintain sufficient above‐ground tissue hydration during winter. Therefore, our findings provide new insights into the water allocation dynamics of temperate trees during early spring.
Jasmonates Regulate Auxin‐Mediated Root Growth Inhibition in Response to Rhizospheric pH in Arabidopsis thaliana
Authors: Singh Ajit Pal , Kanwar Rajni , Pandey Ajay K. 
Published: October 2024

ABSTRACTRhizospheric pH, an important environmental cue, severely impacts plant growth and fitness, therefore, has emerged as a major determinant of crop productivity. Despite numerous attempts, the key questions related to plants response against rhizospheric pH remains largely elusive. The present study provides a mechanistic framework for rhizospheric pH‐mediated root growth inhibition (RGI). Utilizing various genetic resources combined with pharmacological agents and high‐resolution confocal microscopy, the study provides direct evidence for the involvement of jasmonates and auxin in rhizospheric pH‐mediated RGI. We show that auxin maxima at root tip is tightly regulated by the rhizospheric pH. In contrast, jasmonates (JAs) abundance inversely correlates with rhizospheric pH. Furthermore, JA‐mediated regulation of auxin maxima through GRETCHEN HAGEN 3 (GH3) family genes explains the pattern of RGI observed over the range of rhizospheric pH. Our findings revealed auxin as the key regulator of RGI during severe pH conditions, while JAs antagonistically regulate auxin response against rhizospheric pH.
Perinatal mortality among term births: Informing decisions about singleton early term births in Western Australia
Authors: Berman Ye’elah E. , Newnham John P. , Nathan Elizabeth A. , Doherty Dorota A. , Brown Kiarna , Ward Sarah V. 
Published: October 2024

AbstractBackgroundTo minimise the risk of perinatal mortality, clinicians and expectant mothers must understand the risks and benefits associated with continuing the pregnancy.ObjectivesReport the gestation‐specific risk of perinatal mortality at term.MethodsPopulation‐based cohort study using linked health data to identify all singleton births at gestations 37–41 weeks, in Western Australia (WA) from 2009 to 2019. Lifetable analysis was used to combine the risk of each type of perinatal mortality and calculate the cumulative risk of perinatal mortality, termed the perinatal risk index (PRI). Rates of antepartum and intrapartum stillbirth and neonatal death, as well as the PRI, were examined for each gestational week at term by non‐Aboriginal and Aboriginal ethnicity. For non‐Aboriginal women, rates were also examined by time‐period (pre‐ vs. post‐WA Preterm Birth Prevention Initiative (the Initiative) rollout), primiparity, and obstetric risk.ResultsThere were 332,084 singleton term births, including 60 perinatal deaths to Aboriginal mothers (3.2 deaths per 1000 births to Aboriginal mothers) and 399 perinatal deaths to non‐Aboriginal mothers (1.3 deaths per 1000 births to non‐Aboriginal mothers). For non‐Aboriginal women, the PRI was at its lowest (PRI 0.80, 95% CI 0.61, 1.00) at 39 weeks gestation. For Aboriginal women, it was at its lowest at 38 weeks (PRI 2.43, 95% CI 0.48, 4.39) with similar risk at 39 weeks (PRI 2.68, 95% CI 1.22, 4.14). The PRI increased steadily after 39 weeks gestation. The risk of perinatal mortality was higher among Aboriginal women. The gestation‐specific perinatal mortality rates were similar by the time‐period, primiparity and obstetric risk.ConclusionsThe gestational ages at term associated with the lowest risk of perinatal mortality reinforce that the recommendation not to deliver before 39 weeks without medical indication is applicable to both Aboriginal and non‐Aboriginal women giving birth in WA. There was no increase in the perinatal mortality rate associated with the introduction of the Initiative.
Asymptotically faster estimation of high‐dimensional additive models using subspace learning
Authors: He Kejun , He Shiyuan , Huang Jianhua Z. 
Published: October 2024

AbstractAs a commonly used nonparametric model to overcome the curse of dimensionality, the additive model continually attracts attentions of researchers. Our recent work (He et al., 2022) proposed to reduce the number of unknown functions to be estimated through learning an adaptive subspace shared by the additive component functions. Equipped with an efficient algorithm, our proposed reduced additive model outperforms the state‐of‐the‐art alternatives in numerical studies. However, the asymptotic properties of the proposed estimators have not been explored and the empirical findings are short of theoretical explanation. In this work, we fill in the theoretical gap by showing the resulting estimator has faster convergence rate than the estimation without subspace learning; and this is true even when the reduced additive model is only an approximation, provided that the subspace approximation error is small. Moreover, the proposed method is able to consistently identify the relevant predictors. The developed theoretical results back up the earlier empirical findings.
The Rate of Leg Fat Oxidation Is Not Attenuated During Incremental Intensity One‐Leg Knee Extensor Exercise
Authors: Helge J. W. , Shannon C. E. , Stallknecht B. , Stephens F. B. , Greenhaff P. L. , Dela F. 
Published: October 2024

ABSTRACTIt is not clear if fat oxidation is attenuated at higher exercise intensities, when exercising with a small muscle mass, and therefore, we studied leg fat oxidation during graded one‐leg exercise. Ten males (age: 27 ± 2 years, body mass: 82 ± 3 kg, BMI: 24 ± 1 kg m−2, V̇O2max: 49 ± 2 mL min−1 kg−1) performed one‐leg exercise at 25% of maximal workload (Wmax) for 30 min, followed by 120‐min exercise at 55% Wmax with the contralateral leg, and finally 30‐min exercise at 85% Wmax with the first leg. Blood was sampled from an artery and both femoral veins, and blood flow was determined using Doppler ultrasound. Muscle biopsies were obtained before and after 30 min at each workload. One‐way RM ANOVA was applied to determine the impact of exercise intensity. Data are expressed as mean ± SEM. From rest through exercise average blood flow (0.4 ± 0.1, 2.1 ± 0.1, 2.6 ± 0.2, 3.7 ± 0.2 L min−1) and oxygen uptake across the leg (0.03 ± 0.01, 0.23 ± 0.02, 0.35 ± 0.03, 0.53 ± 0.04 L min−1) increased with exercise intensity (p < 0.001). Leg RQ (0.76 ± 0.04, 0.86 ± 0.02,0.87 ± 0.01, 0.92 ± 0.01, p < 0.001), leg plasma FA uptake (2 ± 2, 46 ± 8,83 ± 9, 114 ± 16 μmol min−1; p < 0.001) and rate of leg fat oxidation (0.016 ± 0.005, 0.062 ± 0.012, 0.075 ± 0.011, 0.084 ± 0.018 g min−1, p < 0.007) increased with exercise intensity. Muscle‐free carnitine content was unchanged from rest at 25% Wmax and decreased after 30 min exercise at 55% and 85% Wmax (17.4 ± 1.6, 16.6 ± 0.7, 14.5 ± 1.2, 10.5 ± 1.0 mmol/kg dry muscle, respectively; p < 0.006). During incremental one‐leg exercise, the rate of leg fat oxidation was not attenuated with increasing exercise intensity, probably due to an insufficient muscle metabolic stress response.
Current–Voltage–Friction Characteristics of Grease in Electromechanically Loaded Sliding Contacts
Authors: Balakrishna G. V. , Gnanamoorthy R. 
Published: October 2024

Abstract
Electromechanically loaded contacts, which have relative motion between the contacting parts, experience severe damage compared to mechanically loaded contacts. The electromechanical environment occurs when different types of current flow through the bearings of traction motors due to the usage of electronic speed control devices. The current passage through the contact depends on the voltage potential developed across the contact. Grease is commonly used as a lubricant, and degradation and evaporation of lubricant due to the joule heating effect are concerns in electromechanical contacts. This study reports the current–voltage–friction characteristics of lithium mineral oil grease using a ball-on-disk configuration under combined electrical and mechanical loading. The characteristics indicated a transition of the lubricated contact from a non-conducting state to a conducting state with increased applied voltage. Two critical voltages are identified: one where the friction is observed to rise and the other where the current flow rapidly increases, leading to accelerated damage to the lubricant by inducing a significantly high temperature. The study helps in identifying permissible voltage levels for operating bearings safely from the perspective of grease lubricant using simplified ball-on-disk experiments.
Heat Transfer Characteristics of Methane–Air-Premixed Jet Flames With Flat/Hemispherical Walls
Authors: Zeng Chun , Pan Jianfeng , Hua Jinpeng , Yang Wenming , Li Zhongjia , Nauman Muhammad 
Published: October 2024

Abstract
The in-depth study of the mutual coupling between the flame and the wall can significantly enhance the efficiency of actual combustion devices. A two-dimensional numerical model was established for the heat transfer characteristics of methane–air-premixed jet flames on flat and hemispherical walls, respectively. An examination of the effects of wall shape on the heat transfer characteristics of methane/air flames was conducted as a function of the equivalence ratio (ϕ = 0.9–1.5), the mixture Reynolds number (Re = 300–800), and the burner-to-plate distance (H/d = 1–6). As the equivalence ratio and Reynolds number increase, the flame temperature increases on the surface near the wall, and the temperature near the flame centerline is higher under the influence of a hemispherical wall than it is under the influence of a plate. In addition, the wall's heat flux increases as both the equivalence ratio and the Reynolds number increase. It is observed that the heat flux of the hemispherical wall is greater than that of the flat plate near the stagnation point, whereas it is smaller at a distance from the stagnation point. Due to the burner-to-plate distance, thermal efficiency is maximized when the flame-premixed cone contacts the impact surface, which is the desired condition for optimal performance. Due to different operating conditions, the efficiency of heat transfer is always higher under the action of a flat plate than under the action of a hemispherical wall.
Offline and Online Exergy-Based Strategies for Hybrid Electric Vehicles
Authors: Acquarone Matteo , Pozzato Gabriele , James Corey , Onori Simona 
Published: October 2024

Abstract
Exergy-based control strategies for ground hybrid electric vehicles (HEVs) enable to pursue unconventional optimization goals that are inaccessible when standard energy-based modeling frameworks based on fuel consumption minimization are used. In this work, we formulate and solve offline and online exergy-based optimization strategies for military HEVs aimed at the minimization of genset exergy destruction and thermal emissions to increase vehicle efficiency and minimize the risk of thermal imaging detection, respectively. We refer to the offline version of these strategies as exergy minimization strategies (ExMSs). Adaptive ExMSs (A-ExMSs) are then formulated for online implementation. Moreover, charge increasing (CI) ExMSs and A-ExMSs are developed to charge the battery as much as possible during a driving mission that is followed by a silent watch phase. To assess the performance of the proposed strategies, the results obtained by the ExMSs and A-ExMSs are compared to the benchmark solutions obtained by Dynamic Programming.
MVP: a modular viromics pipeline to identify, filter, cluster, annotate, and bin viruses from metagenomes
Authors: Coclet Clément , Camargo Antonio Pedro , Roux Simon 
Published: October 2024

ABSTRACT

While numerous computational frameworks and workflows are available for recovering prokaryote and eukaryote genomes from metagenome data, only a limited number of pipelines are designed specifically for viromics analysis. With many viromics tools developed in the last few years alone, it can be challenging for scientists with limited bioinformatics experience to easily recover, evaluate quality, annotate genes, dereplicate, assign taxonomy, and calculate relative abundance and coverage of viral genomes using state-of-the-art methods and standards. Here, we describe Modular Viromics Pipeline (MVP) v.1.0, a user-friendly pipeline written in Python and providing a simple framework to perform standard viromics analyses. MVP combines multiple tools to enable viral genome identification, characterization of genome quality, filtering, clustering, taxonomic and functional annotation, genome binning, and comprehensive summaries of results that can be used for downstream ecological analyses. Overall, MVP provides a standardized and reproducible pipeline for both extensive and robust characterization of viruses from large-scale sequencing data including metagenomes, metatranscriptomes, viromes, and isolate genomes. As a typical use case, we show how the entire MVP pipeline can be applied to a set of 20 metagenomes from wetland sediments using only 10 modules executed via command lines, leading to the identification of 11,656 viral contigs and 8,145 viral operational taxonomic units (vOTUs) displaying a clear beta-diversity pattern. Further, acting as a dynamic wrapper, MVP is designed to continuously incorporate updates and integrate new tools, ensuring its ongoing relevance in the rapidly evolving field of viromics. MVP is available at
https://gitlab.com/ccoclet/mvp
and as versioned packages in PyPi and Conda.

IMPORTANCE
The significance of our work lies in the development of Modular Viromics Pipeline (MVP), an integrated and user-friendly pipeline tailored exclusively for viromics analyses. MVP stands out due to its modular design, which ensures easy installation, execution, and integration of new tools and databases. By combining state-of-the-art tools such as geNomad and CheckV, MVP provides high-quality viral genome recovery and taxonomy and host assignment, and functional annotation, addressing the limitations of existing pipelines. MVP’s ability to handle diverse sample types, including environmental, human microbiome, and plant-associated samples, makes it a versatile tool for the broader microbiome research community. By standardizing the analysis process and providing easily interpretable results, MVP enables researchers to perform comprehensive studies of viral communities, significantly advancing our understanding of viral ecology and its impact on various ecosystems.
Applying the theoretical domains framework to identify determinants to mental healthcare use among older African Americans with type 2 diabetes: a qualitative study
Authors: Hawkins Jaclynn , Sengupta Srijani , Kloss Katherine A , Schwenzer Claudia , Smith Fonda N , Jones Lenette M , Mitchell Jamie , Nwankwo Robin , Piatt Gretchen A 
Published: October 2024

ObjectivesThere is a paucity of research focused on enhancing access to mental healthcare for older African Americans with type 2 diabetes (T2D), who may be at risk for or living with comorbid depression. This study aims to identify barriers and facilitators to mental healthcare utilisation among this population, guided by the theoretical domains framework (TDF).DesignThis qualitative study involved 30 interviews with older African American adults diagnosed with T2D. The interview questions were aligned with TDF domains to capture participant perspectives on barriers and facilitators to mental healthcare use.SettingInterviews were conducted via telephone by a licensed clinician trained in social work. Each session lasted 60–90 min and was transcribed and analysed.ParticipantsThe study included 30 African American adults (15 males and 15 females), aged 60 and above, living in an urban area in the Midwest.Primary and secondary outcomesThe primary outcome was the identification of themes from participant responses, analysed using thematic content techniques and categorised into TDF constructs. Demographic data served as the secondary outcome.ResultsNine key themes were identified, categorised under major TDF domains and constructs. Significant barriers included (1) systemic racism (‘knowledge’), (2) normalisation of depressive symptoms (‘beliefs about consequences’), (3) perceived stigma (‘beliefs about consequences’) and 4) costs of medications and healthcare (‘environmental context and resources’). Facilitators to seeking mental healthcare included (1) empowerment (‘beliefs about capabilities’), (2) perceived benefits of mental health exams (‘beliefs about consequences’), (3) positive provider experiences (‘reinforcement’), (4) recognition of depressive symptoms as a motivator (‘goals’) and (5) support networks (‘social influences’).Conclusion and implicationsKey findings highlight that fostering positive patient–provider relationships and enhancing self-recognition of depressive symptoms can significantly encourage mental healthcare utilisation among older African Americans with T2D. These findings suggest that future interventions should focus on strengthening these relationships and improving self-awareness to better mental health outcomes.