bluuebunny/crossref_metadata_2025_split huggingface dataset:
ID:2038 THE ROLE OF THE CASPASE FAMILY in PF4-INDUCED APOPTOSIS IN BREAST CANCER IN VIVO STUDY
Authors: Din Tengku Ahmad Damitri Al Astani Tengku 
Published: September 2017

Background: Apoptosis is critical to the development and homeostasis of metazoans. Apoptotic dysregulation leads to various human pathologies including cancer, autoimmune and neurodegenerative disorders. Caspases, sub-members of cysteine proteases, are the central components of apoptotic response. The ELR-negative chemokine platelet factor 4 (PF4) was initially identified as an antiangiogenic agent. It inhibits endothelial cell proliferation, migration and angiogenesis in in vitro and in vivo and was further proving in the in vivo models of human colon carcinoma and murine melanoma xenograft mouse. However, there are no studies (either in-vivo or ex-vivo) which have conclusively demonstrated and characterized the PF4 effects on inducing apoptosis in breast cancer setting and therefore, this becomes the study’s central objective.

Aim: This study aimed to investigate PF4's effects on the apoptosis regulation mediated by caspase -3, -6, -7, -8 and -9 in 1-methyl-1-nitrosourea-induced (MNU) invasive murine breast carcinoma model.
Methods: Breast carcinoma were induced in 60, 21-day-old female Sprague Dawley Rat (SDR) using 1-methyl-1-nitrosourea until its size reached 14.5±0.5 mm. The tumors were divided into two groups: Group 1 (control pre-treated, n=20 and post treated, n=20), and Group 2 (PF4, n=20). PF4 were administered through focal intralesional injections at 20 µg/lesion dosage. After 5 days of treatment, the SDRs were sacrificed. Subsequently the tumor specimens were prepared for haematoxylin and eosin staining. The expression of caspases family members (caspase-3,-6,-7,-8 and-9) were detected using immunohistochemistry. The specimens were morphologically examined and the cell counts with positive caspases expressions were recorded.
Results: There was a significant reduction of tumor size after PF4 treatment compared to the controls. PF4 demonstrated differential effects on downstream effector caspases; both upregulated caspase-3 and -6, and downregulated caspase-7 expression was observed compared to controls. PF4 exhibited significant effects on the three caspases-3, -6 and -7 (p <0.001, p=0.004 and p=0.024, respectively). PF4 had no effect on caspase-8 (p=0.067) and caspase-9 (p=0.061) expressions.
Conclusion: This study found that PF4 significantly reduced the tumor sizes in a short time (less than 3 days) and acts differentially on the caspase-mediated apoptosis pathways and on the downstream levels of the apoptotic machinery.
ID: 1006 Allogeneic adipose -derived mesenchymal stem cell transplantion enhanced the expression of angiogenic factors on mouse acute hindlimb ischemia model
Authors: Vu Ngoc Bich , Le Ha Thi-Ngan , Dao Thuy Thi-Thanh , Phi Lan Thi , Pham Phuc Van , Phan Ngoc Kim , Ta Van Thanh 
Published: September 2017

The cell migration and/or molecular mechanism in healing of damaged vascular or muscle tissue are emerging field with strong research interest worldwide. This study focuses on evaluating the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) in restoring damaged tissue through the migration of cells and the expression of genes related to myocyte regeneration and angiogenesis on hindlimb ischemic mouse model. ADMSCs were labeled with GFP (ADMSC-GFP). The proximal end of the femoral blood vessel of mice (over 6 months old) was ligated at 2 positions then cut between the two ties. Hindlimb ischemic mice were randomly divided to 2 groups. Group 1 (n=36) was injected PBS (100 μl), group 2 (n=36) was transplanted ADMSC-GFP (106 cell/100 μl PBS) near the cutting position. The migration of ADMSC-GFP in hindlimb was analyzed by UVVis system. The expression of genes related to angiogenesis and muscle tissue repairing was quantified by real time RT-PCR. Muscle tissue structure was histological analyzed by H&E staining. The result showed that ADMSC existed in grafted hindlimb during 7 days. Grafted cells migrated to other damaged areas such as thighs and heels. Ischemic hindlimb were stimulated to increasing expression of several angiogeneic genes including Flt-1, Flk-1 Ang-2 in both group. Especially, the expression of Ang-2 and myogenic-related gene MyoD were significantly increased in ADMSC-treated group compared to non-treated group; and increased at 28th day compared to 3rd day. The other factors such as HGF, CD31, Myf5, TGF-β also expressed much more in ADMSC treated group than the another one. Muscle histological structure in ADMSC group were siminar the normal mouse after 28 day. Thus, grafted-ADMSC was able to migrate to other area in injured hindlimb, existed for about 7 days and have significant positive effects on stimulating the expression of myogenic and angiogenesis- related genes.
ID: 1019 Efficiency of the combination of modified acupuncture and motor relearning method in post-stroke patients
Authors: Man Bui Pham Minh , Dieu Thuong Trinh Thi 
Published: September 2017

Background and Objectives: The combination of modern medicine and traditional medicine in the rehabilitation of motor deficit after stroke has shown interesting results. Many studies on modified acupuncture, a combination of modern and traditional techniques, have proven its effectiveness in motor rehabilitation in post-stroke patients. Furthermore, many studies have elucidated the effectiveness of the motor relearning method in the treatment of post-stroke paralysis. Therefore, this study aims to determine whether the combination of modified acupuncture and motor relearning method can improve the treatment results. Method: Multi-centered randomized controlled trial (Traditional Medicine Hospital of Ho Chi Minh City, General Hospital of Soc Trang Province, and People Military Hospital of Soc Trang Province) from July 2014 to July 2015. 66 post-stroke patients were divided into two groups: a control group received modified acupuncture combined with Bobath method and a trial group received modified acupuncture combined with motor relearning method. After six weeks, patients were evaluated according to the Barthel score, 10-hole test, and 10-meter-walk test. Results: The trial group showed better results than the control group did. There was a significant difference between two groups in Barthel score and 10-meter-walk test, but not in the 10-hole test. After treatment, 77.42% patients of the trial group showed improvement while only there are 51.61% in the other group (P < 0.05). Conclusion: The combination of modified acupuncture and motor relearning method is more effective than the combination of modified acupuncture and Bobath method in the rehabilitation of motor deficit after stroke.
ID: 1020 Direct reprogramming of fibroblasts into endothelial progenitor cells by defined factors
Authors: Truong Mai Thi-Hoang , Huynh Oanh Thuy , Phan Liem Hieu , Pham Phuc Van 
Published: September 2017

Background: Endothelial progenitor cells (EPCs) are important progenitor cells in vasculogenesis as well as in tissue engineering. However, few EPCs can be isolated from bone marrow, peripheral blood and umbilical cord blood. Moreover, their in vitro proliferation potential is also limited. Therefore, this study aimed to produce EPCs from direct reprogramming of fibroblasts by transduction with certain specific factors.
Methods: Human fibroblasts were collected from human skin by published protocols. The cells were transduced with 2 viral vectors containing 5 factors, including Oct3/4, Sox2, Klf4, c-Myc (plasmid 1), and VEGFR2 (plasmid 2). Transduced cells were treated with endothelial cell medium for 21 days. The cells were analyzed for expression of Oct3/4, Sox3, Klf4, c-Myc and VEGFR2 at day 5, and for EPC phenotype at day 21.
Results: The results showed that after 5 days of transduction, fibroblasts acquired partial pluripotency. After 21 days of transduction and culture in endothelial cell medium, the cells exhibited endothelial markers (e.g. CD31 and VEGFR2) and formed blood vessel-like capillaries.
Conclusion: Our findings suggest another strategy for direct reprogramming of fibroblasts into EPCs.
ID: 1021 Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector
Authors: Huynh Oanh Thuy , Truong Mai Thi-Hoang , Pham Phuc Van 
Published: September 2017

Background: Embryonic stem cells are pluripotent, thus capable of differentiating into all types of cells derived from the three germ layers. However, the application of embryonic stem cells (ESCs) for preclinical and clinical studies is difficult due to ethical concerns. Induced pluripotent stem cells (iPSCs) are derived from differentiation and have many ESC characteristics. The study herein examines the production of iPSCs from reprogramming of mouse embryonic fibroblasts (MEFs) via transduction with defined factors.
Methods: MEFs were collected from mouse embryos via a previously published protocol. The cells were transduced with a single polycistronic viral vector encoding mouse cDNAs of Oct3/4, Sox2, Klf4 and c-Myc. Transduced cells were treated and sub- cultured with ESC medium. The cells were evaluated as iPSCs with specific morphology, and expression SSEA-1, Oct3/4, Sox2 and Nanog. In addition, they were also evaluated for pluripotency by assessing alkaline phosphatase (AP) activity and in vivo teratoma formation.
Results: Under the reprogrammed conditions, the transduced cells displayed a change in morphology, forming ESC-like clusters. These cell clusters strongly expressed pluripotent markers as well as ESC-specific genes. Furthermore, the colonies exhibited higher AP activity and formed teratomas when injected into the murine testis.
Conclusion: The study herein suggests that MEFs can be reprogrammed into iPSCs using a polycistronic viral vector encoding mouse cDNAs for Oct3/4, Sox2, Klf4 and c- Myc
ID: 1028 Comparison of HER2 status in gastric adenocarcinoma patients between gastrectomy specimens and matched endoscopic specimens
Authors: Hung Cuong Pham , Nu Hoang Yen Tran 
Published: September 2017

Background: To evaluate the concordance between HER2 status in both surgical specimens and matched endoscopic specimens of gastric adenocarcinoma patients.
Methods: Records of 52 patients with gastric adenocarcinoma diagnosed by upper GI endoscopy, treated by gastrectomy and Her2 status assessed (by immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH)) from June 2014 through July 2016 in HCMC Oncology Hospital were studied and presented in case series. Her2-positive status was defined by a score 3+ in IHC, or 2+ with a positive FISH test.
Results: + Her2-positive status was identified in 9.6%.
+ The concordance of Her2 status between endoscopic biopsy and surgical specimens was 98% (Kappa=0.879).
Conclusion: + There was a very high concordance between the Her2 status results performed on surgical specimens and matched endoscopic specimens.
+ Her2 status assessed on endoscopic biopsy specimens could be reliable for treatment decisions using anti-Her2 agents in patients with advanced gastric carcinoma
ID: 1030 Observing an expression of TNF-α, IL10 and HBEGF on peripheral blood mononuclear cells (CD14+) of a guttate psoriatic patient at Ho Chi Minh City
Authors: Viet Nguyen Hong , Hoa Pham Cuc 
Published: September 2017

Psoriasis is a chronic autoimmune disease in dermatology. The experiment collected peripheral blood samples from cohorts. These samples were treated and sorted mononuclear leukocytes with LPS receptors or CD14. Then, they were stimulated by a lysis of Streptococcus pneumonia in 24 hours. These cells were harvested in 0, 1, 24, 48, 72,120 (day 5) and 168 (day 7) hours and were isolated total mRNA. All mRNA samples were converted into the cDNA samples which were utilised to indirectly evaluate the cytokine expression of TNF-α, IL10 and Heparin-binding EGF-like growth factor (HBEGF) on the mononuclear cells (CD14+) in the period shown. Regarding result of the psoriatic patient, three cytokines abnormally prolonged after the 24 hours induction. The signals of TNF-α, IL10 and HBEGF expanded throughout 48 hours,5 days and 7 days, respectively. In addition, a band (627bp) was obtained in from PCR products of IL10. Furthermore, sequencing the 627bp band shows that it was homologous to a fragment on chromosome 2. Meanwhile, the results of those cytokines on a cohort without non-psoriasis were slightly exposed in around 48 hours. In conclusion, the mononuclear cells (CD14+) in the cohort with guttate psoriasis is highly more sensitive with a lysis of Streptococcus pneumonia than the non-psoriatic cohort in TNF-α expression, and IL10 and HBEGF of the psoriatic one are also simultaneous and extended during the inflammation and wound healing. Furthermore, the 620bp band might relate to edit progressing pre-mRNA IL10 in the guttate psoriasis.
ID: 1043 Study value of 12-lead ECG for the left free wall of accessory pathways diagnosing in patients with typical Wolff-Parkinson-White Syndrome
Authors: Si Chu Dung , Khanh Pham Quoc , Dong Tran Van 
Published: September 2017

Objectives: This study was designed characteristics of 12-lead Electrocardiogram (ECG) for the left free wall lateral of accessory pathway (AP) localization in the typical WPW syndrome to develop a new algorithm ECG for localizing APs, and to test the accuracy of the algorithm prospectively.
Methods: We studied 129 patients, in 84 patients with typical WPW syndrome have a single anterograde with the localization of APs identified by successful radiofrequency catheter ablation (RCFA) to develop a new ECG algorithm for the left free wall of localizing APs. Then this algorithm was tested prospectively in 45 patients were compared with the location of AP’s successful ablation by RCFA.
Results: We found that the 12 lead ECG parameters in typical WPW syndrome such as delta wave polarity in V1, R/S ratio in V1, the transition of the QRS complex, delta wave polarity in inferior leads in diagnosis for the localization of APs by with high accuracy predicted from 74,5% to 100%, and for development of a new ECG algorithm. Then the following 45 patients were prospectively evaluated by the new derived algorithm for the left free wall pathways with high sensitivity and specificity from 75% to 100%.
Conclusion: 12-lead ECG parameters in typical WPW syndrome closely related to left free wall of APs localization, in order to develop the new ECG algorithm by parameters as above; and can be used to a new ECG algorithm in predicting the location APs with high accuracy.
ID: 1067 The Effect of Oocyte-Cumuls Cell Complexes on Maturation Rates and Pronuclear Formation After Pathenogenetic Activation
Authors: Nhan Bui Thanh , Tram Nguyen Dang Bich , Doan Phung Ngoc Minh , Thuy Bui Hong , Thuan Nguyen Van 
Published: September 2017

Cumulus cells are integral in oocyte growth, maturation, pronuclear formation as well as preimplantation development after fertilization. In this study, we investigated the effect of different cumulus morphology on maturation rates and pronuclear formation after parthenogenetic activation. Oocyte-cumulus cell complexes (OCCs) were classified into three grades: (A) over 3 layers, (B) 2–3 layers, (C) less than 1 layer or incomplete. Our results showed that group A and B achieved high maturation rates (84.4% and 82.5%) whereas group C were 45.7%. In a similar pattern, after parthenogenetic activation, the rate of pronuclear formation of group A and B were namely 65.5% and 52%, while that of group C was only 36.4%. Moreover, we also examined the impact of duration of in vitro maturation on the rate of nuclear formation after activation. We found that 25-hour would result in the highest rate (55.5%) while only 4.44% in group of 30-hour, which was possibly caused by the aging of oocytes. In conclusion, this study found that OCCs with more than 2 layers of cumulus cells could reach the high maturation rates, finally oocytes activated at 25 hours resulted in high rate of pronuclear formation in bovine.
ID: 1071 Establishing an early-stage femoral head necrosis model of rabbit using methylprednisolone and Complete Freund's Adjuvant
Authors: Phi Lan Thi , Dao Thuy Thi-Thanh , Nguyen Khanh Dinh-Van , Pham Phuc Van , Bui Khanh Hong-Thien , Vu Ngoc Bich 
Published: September 2017

Background: Pathological animal models provide the foundation for developing new methods for treating. This research aims to establish a rabbit model of femoral head necrosis. Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylpresnisolone combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. Group A (n=10) was given an intramuscular injection of 0.5 mg/kg (CFA) and 40mg/kg methylprednisolone (MPS). Group B (n=6) was received normal saline at the same location and same volume as those in Group A. The efficiency of ONFH rabbit model was assessed at 7 weeks after the last injection. Body weight was weighed. The histological structure of head femoral and bone were deteded by H&E staining. The empty lacuna was counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. The results showed that there was no difference in body weight change of rabbits between two groups. However, the bone morphology and cartilage surface of femoral head were abnormalities at group A. The percentage of empty osteocyte lacunae were significantly higher in Group A than Group B. Chondrocyte degeneration and fibrocartilage expression were observed at Group A. Compare to group B, Group A had less ink-stained blood vessels. Moreover, the fraction of bone marrow-derived mononuclear at S phase and G2/M phase of the cell cycle was significantly decreased in group A. Thus, CFA combined with MPS can be used to establishing an early-stage femoral head necrosis model of rabbit.
ID: 1075 Concentration and κ/λ ratio of serum free light chain in multiple myeloma patients at Cho Ray Hospital
Authors: Phan Hoang Cong , Phan Thang Thanh , Ho Toan Trong , Tran Thu Bich , MCB Thanh Thanh , Nguyen Son Truong 
Published: September 2017

Introduction: multiple myeloma (MM) is a B cell malignancy which characterized by accumulation of plasmocyte in bone marrow, immunoglobulins and free light chain in serum (sFLC). According to IMMWG (International multiple myeloma working group), sFLC is a criterion in diagnosis of MM. In this study, we investigate the concentration and κ/λ ratio of sFLC in newly diagnosed MM patients at Cho Ray hospital. Methods: cross-sectional study. Serum samples of 36 newly diagnosed MM patients were analyzed by Binding Site Freelite method to measure the amount of κ and λ sFLC.

Results: the median age of 36 MM patients was 61 yrs (41 – 88 yrs), with the male/female ratio was 1.2/1. MM stage III, stage II and stage I accounting for 63.9% (23/36), 27.8% (10/36), and 8.3% (3/36), respectively. Clonal IgG, IgA and IgE MM accounting for 61.1% (22/36), 30.6% (11/36), and 8.3% (3/36), respectively. We reported the median of plasmocyte in 36 cases was 35.4% (95%CI: 27.6 – 43.3). The median of concentration of κ sFLC, λ sFLC, and involved/uninvolved sFLC ratio were 1601.8 mg/L (95%CI: 104.9 – 3098.7), 900.1 mg/L (95%CI: 10.7 – 1789.5), and 191.0 (95%CI: 87.6 – 294.4), respectively. The abnormally high in sFLC concentration was reported in 97.2% MM cases (35/36), in which 55.6% cases (20/36) had increased κ sFLC, 19.4% cases (7/36) had increased λ sFLC, and 22.2% cases (8/36) had increased both κ+λ sFLC concentration. Increased involved/uninvolved sFLC ratio was found in 94.4% MM cases (34/36), in which 36.1% cases (13/36) had sFLC ratio > 100, with all involved sFLC concentration > 100 mg/L. In 3 cases of MM with kidney failure, 2 cases had increased both κ+λ sFLC, 1 case had increased κ sFLC concentration; and all of 3 cases had involved sFLC level > 5300 mg/L with sFLC ratio > 100. No significant difference of sFLC concentration and sFLC ratio among gender, stage of disease, Ig clone, or plasmocyte percent in bone marrow of MM patients. Conclusions: due to the abnormally high of sFLC concentration and sFLC ratio in MM, it is necessary to monitor frequently these parameters during treatment to prevent the risk of kidney failure for patients.
ID: 1083 Targeting the production of oncogenic miRNAs using synthetic small molecules
Authors: Tran Anh Thi-Phuong , Vo Duc Huy , Giorgio Audrey Di , Duca Maria 
Published: September 2017

MicroRNAs (miRNAs or miRs) are a class of evolutionary conserved small non-coding RNAs that act as post-transcriptional regulators of gene expression. A wide number of studies has shown that the aberrant expression of miRNAs could be responsible for initiation and development of human cancers. Most of these deregulated miRNAs are overexpressed, thus being oncogenic. For these reasons, the inhibition of oncogenic miRNAs function or production would be a very promising approach for the development of new anticancer therapies [1].
The purpose of this work is the discovery of small-molecule drugs targeting the precursors of specific oncogenic miRNAs thus modulating their production. We have focused our attention on miRNA-372 and miRNA- 373 that are implicated in various cancers. For example, these two oncogenics (pre-miRNAs 372 and pre-miRNAs 373): two stem-loop structured RNAs that lead to mature miRNAs after cleavage by the enzyme Dicer. In the aim of inhibiting this biogenesis step and based on our previous works [2], we synthesized a novel series of RNA ligands composed of two different domains: (i) 2-deoxystreptamine (2-DOS) known as RNA-interacting group due to its role as a central scaffold of any known aminoglycosides [3] and (ii) aromatic or heteroaromatic groups that should improve affinity and selectivity of these ligands for the targeted RNAs. These two domains have been conjugated through carbamate, triazole and ether bonds in order to develop a simple and straightforward synthetic methodology and obtain a large number of diversified compounds.

We obtained a library of 16 compounds which will be evaluated in vitro in order to identify the strongest RNA ligands that could be able to inhibit the production of the targeted oncogenic miRNAs and eventually lead to the inhibition of cancer cells proliferation.