bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of rapid test kits to assess household coverage of iodized salt
Authors: Gorstein Jonathan , van der Haar Frits , Codling Karen , Houston Robin , Knowles Jacky , Timmer Arnold 
Published: May 2016

AbstractObjectiveThe main indicator adopted to track universal salt iodization has been the coverage of adequately iodized salt in households. Rapid test kits (RTK) have been included in household surveys to test the iodine content in salt. However, laboratory studies of their performance have concluded that RTK are reliable only to distinguish between the presence and absence of iodine in salt, but not to determine whether salt is adequately iodized. The aim of the current paper was to examine the performance of RTK under field conditions and to recommend their most appropriate use in household surveys.DesignStandard performance characteristics of the ability of RTK to detect the iodine content in salt at 0 mg/kg (salt with no iodine), 5 mg/kg (salt with any added iodine) and 15 mg/kg (‘adequately’ iodized salt) were calculated. Our analysis employed the agreement rate (AR) as a preferred metric of RTK performance.Setting/SubjectsTwenty-five data sets from eighteen population surveys which assessed household iodized salt by both the RTK and a quantitative method (i.e. titration or WYD Checker) were obtained from Asian (nineteen data sets), African (five) and European (one) countries.ResultsIn detecting iodine in salt at 0 mg/kg, the RTK had an AR>90 % in eight of twenty-three surveys, while eight surveys had an AR<80 %. When the RTK was used for detecting adequately iodized salt, the AR decreased significantly, with only one of fourteen surveys achieving an AR>90 %.ConclusionsThe RTK is not suited for assessment of adequately iodized salt coverage. Quantitative assessment, such as by titration or WYD Checker, is necessary for estimates of adequately iodized salt coverage.
The humanitarian impact and implications of nuclear test explosions in the Pacific region
Authors: Ruff Tilman A. 
Published: May 2016

AbstractThe people of the Pacific region have suffered widespread and persisting radioactive contamination, displacement and transgenerational harm from nuclear test explosions. This paper reviews radiation health effects and the global impacts of nuclear testing, as context for the health and environmental consequences of nuclear test explosions in Australia, the Marshall Islands, the central Pacific and French Polynesia. The resulting humanitarian needs include recognition, accountability, monitoring, care, compensation and remediation. Treaty architecture to comprehensively prohibit nuclear weapons and provide for their elimination is considered the most promising way to durably end nuclear testing. Evidence of the humanitarian impacts of nuclear tests, and survivor testimony, can contribute towards fulfilling the humanitarian imperative to eradicate nuclear weapons.
Hypertensive disorders during pregnancy and health outcomes in the offspring: a systematic review
Authors: Pinheiro T. V. , Brunetto S. , Ramos J. G. L. , Bernardi J. R. , Goldani M. Z. 
Published: May 2016

The hypertensive disorders of pregnancy complicate up to 10% of pregnancies worldwide and are a leading cause of maternal, foetal, and neonatal morbidity and mortality. The aim of this study was to present an overview of recent studies addressing offspring’s medium and long-term health outcomes after intrauterine exposure to maternal hypertension. A search on PubMed/MEDLINE and Bireme databases was conducted to identify observational studies that reported any offspring outcome measured after the 6th month of life. The search was limited to studies published after May 2008. Forty-five articles were included and categorized into four groups of outcomes: cardiovascular, immune, metabolic and behavioural/neurological effects. According to our findings, hypertensive disorders of pregnancy had an overall negative impact on offspring’s cardiovascular, immune and neurological health, although not all parameters analysed in each group had consistent results among studies. The most prominent and reliable associations were verified between gestational hypertension and higher offspring’s blood pressure and between preeclampsia and offspring’s lower cognitive functioning. In the metabolic outcomes, body composition had conflicting results among papers, while all studies that examined blood biomarkers showed no evidence that preeclampsia or gestational hypertension could be associated with an alteration of this metabolic outcomes. Most included studies were highly heterogeneous regarding the measure of outcomes and covariables used for adjustments. Future studies should consider using the same protocols and cut-off points already published so that results can be better compared and summarized.This review was registered in PROSPERO. Registration number: CRD42015020838
Urdu Translation and Validation of Short Muslim Practice and Belief Scale in Pakistan
Authors: Ghayas Saba , Batool Syeda Shahida 
Published: May 2016

AbstractThe present study was conducted to translate and validate the Short Muslim Practice and Belief Scale (SMPBS) (AlMarri, Oei, & Al-Adawi, 2009) to have a culturally equivalent and linguistically accurate Urdu version for use in Pakistan. Forward backward translation method was used for translation followed by tryout of the scale. Urdu translated version of the scale was completed by 500 participants of different age groups (M= 36.5,SD= 10.1). Based on a confirmatory factor analysis (CFA), results confirmed that a two-factor model with the factors practice and belief provided an excellent fit to the data with chi square 55.96 (df= 26,p> .05), CFI =.96, GFI = .97, and RMSEA = .04. Cronbach’s alpha reliability coefficient was .78. Cross-language validation determined on a sample of 60 participants was satisfactory. Convergent validity of the scale was established by finding its significant positive correlation with religious practice and belief scale (r= .64,p< .001) and significant negative correlation with depression (r= –.18,p< .001) revealed its divergent validity.
Microbial regulation of the soil carbon cycle: evidence from gene–enzyme relationships
Authors: Trivedi Pankaj , Delgado-Baquerizo Manuel , Trivedi Chanda , Hu Hangwei , Anderson Ian C , Jeffries Thomas C , Zhou Jizhong , Singh Brajesh K 
Published: May 2016

AbstractA lack of empirical evidence for the microbial regulation of ecosystem processes, including carbon (C) degradation, hinders our ability to develop a framework to directly incorporate the genetic composition of microbial communities in the enzyme-driven Earth system models. Herein we evaluated the linkage between microbial functional genes and extracellular enzyme activity in soil samples collected across three geographical regions of Australia. We found a strong relationship between different functional genes and their corresponding enzyme activities. This relationship was maintained after considering microbial community structure, total C and soil pH using structural equation modelling. Results showed that the variations in the activity of enzymes involved in C degradation were predicted by the functional gene abundance of the soil microbial community (R2&gt;0.90 in all cases). Our findings provide a strong framework for improved predictions on soil C dynamics that could be achieved by adopting a gene-centric approach incorporating the abundance of functional genes into process models.
Caloric restriction blocks neuropathology and motor deficits in Machado–Joseph disease mouse models through SIRT1 pathway
Authors: Cunha-Santos Janete , Duarte-Neves Joana , Carmona Vitor , Guarente Leonard , Pereira de Almeida Luís , Cavadas Cláudia 
Published: May 2016

AbstractMachado–Joseph disease (MJD) is a neurodegenerative disorder characterized by an abnormal expansion of the CAG triplet in the ATXN3 gene, translating into a polyglutamine tract within the ataxin-3 protein. The available treatments only ameliorate symptomatology and do not block disease progression. In this study we find that caloric restriction dramatically rescues the motor incoordination, imbalance and the associated neuropathology in transgenic MJD mice. We further show that caloric restriction rescues SIRT1 levels in transgenic MJD mice, whereas silencing SIRT1 is sufficient to prevent the beneficial effects on MJD pathology. In addition, the re-establishment of SIRT1 levels in MJD mouse model, through the gene delivery approach, significantly ameliorates neuropathology, reducing neuroinflammation and activating autophagy. Furthermore, the pharmacological activation of SIRT1 with resveratrol significantly reduces motor incoordination of MJD mice. The pharmacological SIRT1 activation could provide important benefits to treat MJD patients.
Genomics reveals historic and contemporary transmission dynamics of a bacterial disease among wildlife and livestock
Authors: Kamath Pauline L. , Foster Jeffrey T. , Drees Kevin P. , Luikart Gordon , Quance Christine , Anderson Neil J. , Clarke P. Ryan , Cole Eric K. , Drew Mark L. , Edwards William H. , Rhyan Jack C. , Treanor John J. , Wallen Rick L. , White Patrick J. , Robbe-Austerman Suelee , Cross Paul C. 
Published: May 2016

AbstractWhole-genome sequencing has provided fundamental insights into infectious disease epidemiology, but has rarely been used for examining transmission dynamics of a bacterial pathogen in wildlife. In the Greater Yellowstone Ecosystem (GYE), outbreaks of brucellosis have increased in cattle along with rising seroprevalence in elk. Here we use a genomic approach to examine Brucella abortus evolution, cross-species transmission and spatial spread in the GYE. We find that brucellosis was introduced into wildlife in this region at least five times. The diffusion rate varies among Brucella lineages (∼3 to 8 km per year) and over time. We also estimate 12 host transitions from bison to elk, and 5 from elk to bison. Our results support the notion that free-ranging elk are currently a self-sustaining brucellosis reservoir and the source of livestock infections, and that control measures in bison are unlikely to affect the dynamics of unrelated strains circulating in nearby elk populations.
Synthesis of acetic acid via methanol hydrocarboxylation with CO2 and H2
Authors: Qian Qingli , Zhang Jingjing , Cui Meng , Han Buxing 
Published: May 2016

AbstractAcetic acid is an important bulk chemical that is currently produced via methanol carbonylation using fossil based CO. Synthesis of acetic acid from the renewable and cheap CO2 is of great importance, but state of the art routes encounter difficulties, especially in reaction selectivity and activity. Here we report a route to produce acetic acid from CO2, methanol and H2. The reaction can be efficiently catalysed by Ru–Rh bimetallic catalyst using imidazole as the ligand and LiI as the promoter in 1,3-dimethyl-2-imidazolidinone (DMI) solvent. It is confirmed that methanol is hydrocarboxylated into acetic acid by CO2 and H2, which accounts for the outstanding reaction results. The reaction mechanism is proposed based on the control experiments. The strategy opens a new way for acetic acid production and CO2 transformation, and represents a significant progress in synthetic chemistry.
The preprotein translocase YidC controls respiratory metabolism in Mycobacterium tuberculosis
Authors: Thakur Preeti , Gantasala Nagavara Prasad , Choudhary Eira , Singh Nirpendra , Abdin Malik Zainul , Agarwal Nisheeth 
Published: May 2016

AbstractThe YidC–Oxa1–Alb3 preprotein translocases play a vital role in membrane insertion of proteins in eukaryotes and bacteria. In a recent study we observed that Rv3921c, which encodes putative YidC translocase in Mycobacterium tuberculosis (Mtb), is essential for in vitro growth of bacteria. However, the exact function of this particular protein remains to identify in mycobacterial pathogens. By performing a systematic study here we show that YidC of Mtb is an envelope protein, which is required for production of ATP and maintenance of cellular redox balance. Drastic effects of depletion of Rv3921c on the expression of hypoxic genes, ATP synthases, and many proteins of central metabolic and respiratory pathways shed a significant light on the function of YidC towards controlling respiratory metabolism in Mtb. Association of YidC with proteins such as succinate dehydrogenases and ubiquinol-cytochrome C reductase further confirms its role in respiration. Finally we demonstrate that YidC is required for the intracellular survival of Mtb in human macrophages.
From radioimmunoassay to mass spectrometry: a new method to quantify orexin-A (hypocretin-1) in cerebrospinal fluid
Authors: Hirtz Christophe , Vialaret Jérôme , Gabelle Audrey , Nowak Nora , Dauvilliers Yves , Lehmann Sylvain 
Published: May 2016

AbstractI125 radioimmunoassay (RIA) is currently the standard technique for quantifying cerebrospinal fluid (CSF) orexin-A/hypocretin-1, a biomarker used to diagnose narcolepsy type 1. However, orexin-A RIA is liable to undergo cross-reactions with matrix constituents generating interference, high variability between batches, low precision and accuracy, and requires special radioactivity precautions. Here we developed the first quantitative mass spectrometry assay of orexin-A based on a multiple reaction monitoring (MRM) approach. This method was tested in keeping with the Clinical and Laboratory Standards Institute (CLSI) guidelines and its clinical relevance was confirmed by comparing patients with narcolepsy type 1 versus patients with other neurological conditions. The results obtained using MRM and RIA methods were highly correlated, and Bland–Altman analysis established their interchangeability. However, the MRM values had a wider distribution and were 2.5 time lower than the RIA findings. In conclusion, this method of assay provides a useful alternative to RIA to quantify orexin-A, and may well replace it not only in narcolepsy type 1, but also in the increasing number of pathologies in which the quantification of this analyte is relevant.
Specific immunotherapy plus Clostridium butyricum alleviates ulcerative colitis in patients with food allergy
Authors: Bin Lan  , Yang Fan , Lu Dong , Lin Zhenlv 
Published: May 2016

AbstractThe aberrant T cell activation plays an important role in the pathogenesis of intestinal inflammation, such as ulcerative colitis (UC). C. butyricum (Cb) is a probiotic and has been employed in the treatment of immune diseases. This study tests a hypothesis that specific immunotherapy (SIT) plus oral Cb (an over-the-counter probiotic) alleviates the UC symptoms. In this study, we conducted a randomized, double-blind, clinical study at our hospital. A total of 80 patients with relapsing-remitting ulcerative colitis and high levels of specific IgE antibody was randomly divided into 4 groups and were treated with SIT or/and Cb, or placebo, respectively for 1 year. The results showed that a food antigen-specific Th2 polarization immune response was observed in UC patients with food allergy (FA). The frequency of regulatory B cells was significantly less in UC patients with FA as compared with healthy subjects. The UC patients with FA were treated with SIT and Cb showed significant amelioration of UC clinical symptoms, reduction of using UC-control medicines and suppression of the skewed Th2 polarization, which did not occur in those treated with either SIT alone, or Cb alone, or placebo. In conclusion, combination of SIT and Cb efficiently alleviates a fraction of UC patients.
Label-based routing for a family of small-world Farey graphs
Authors: Zhai Yinhu , Wang Yinhe 
Published: May 2016

AbstractWe introduce an informative labelling method for vertices in a family of Farey graphs and deduce a routing algorithm on all the shortest paths between any two vertices in Farey graphs. The label of a vertex is composed of the precise locating position in graphs and the exact time linking to graphs. All the shortest paths routing between any pair of vertices, which number is exactly the product of two Fibonacci numbers, are determined only by their labels and the time complexity of the algorithm is O(n). It is the first algorithm to figure out all the shortest paths between any pair of vertices in a kind of deterministic graphs. For Farey networks, the existence of an efficient routing protocol is of interest to design practical communication algorithms in relation to dynamical processes (including synchronization and structural controllability) and also to understand the underlying mechanisms that have shaped their particular structure.
The cis-acting replication element of the Hepatitis C virus genome recruits host factors that influence viral replication and translation
Authors: Ríos-Marco Pablo , Romero-López Cristina , Berzal-Herranz Alfredo 
Published: May 2016

AbstractThe cis-acting replication element (CRE) of the hepatitis C virus (HCV) RNA genome is a region of conserved sequence and structure at the 3′ end of the open reading frame. It participates in a complex and dynamic RNA-RNA interaction network involving, among others, essential functional domains of the 3′ untranslated region and the internal ribosome entry site located at the 5′ terminus of the viral genome. A proper balance between all these contacts is critical for the control of viral replication and translation, and is likely dependent on host factors. Proteomic analyses identified a collection of proteins from a hepatoma cell line as CRE-interacting candidates. A large fraction of these were RNA-binding proteins sharing highly conserved RNA recognition motifs. The vast majority of these proteins were validated by bioinformatics tools that consider RNA-protein secondary structure. Further characterization of representative proteins indicated that hnRNPA1 and HMGB1 exerted negative effects on viral replication in a subgenomic HCV replication system. Furthermore DDX5 and PARP1 knockdown reduced the HCV IRES activity, suggesting an involvement of these proteins in HCV translation. The identification of all these host factors provides new clues regarding the function of the CRE during viral cycle progression.
LRRK1 is critical in the regulation of B-cell responses and CARMA1-dependent NF-κB activation
Authors: Morimoto Keiko , Baba Yoshihiro , Shinohara Hisaaki , Kang Sujin , Nojima Satoshi , Kimura Tetsuya , Ito Daisuke , Yoshida Yuji , Maeda Yohei , Sarashina-Kida Hana , Nishide Masayuki , Hosokawa Takashi , Kato Yasuhiro , Hayama Yoshitomo , Kinehara Yuhei , Okuno Tatsusada , Takamatsu Hyota , Hirano Toru , Shima Yoshihito , Narazaki Masashi , Kurosaki Tomohiro , Toyofuku Toshihiko , Kumanogoh Atsushi 
Published: May 2016

AbstractB-cell receptor (BCR) signaling plays a critical role in B-cell activation and humoral immunity. In this study, we discovered a critical function of leucine-rich repeat kinase 1 (LRRK1) in BCR-mediated immune responses. Lrrk1−/− mice exhibited altered B1a-cell development and basal immunoglobulin production. In addition, these mice failed to produce IgG3 antibody in response to T cell–independent type 2 antigen due to defects in IgG3 class-switch recombination. Concomitantly, B cells lacking LRRK1 exhibited a profound defect in proliferation and survival upon BCR stimulation, which correlated with impaired BCR-mediated NF-κB activation and reduced expression of NF-κB target genes including Bcl-xL, cyclin D2, and NFATc1/αA. Furthermore, LRRK1 physically interacted and potently synergized with CARMA1 to enhance NF-κB activation. Our results reveal a critical role of LRRK1 in NF-κB signaling in B cells and the humoral immune response.
Association between systemic oxidative stress and visual field damage in open-angle glaucoma
Authors: Tanito Masaki , Kaidzu Sachiko , Takai Yasuyuki , Ohira Akihiro 
Published: May 2016

AbstractLocal and systemic oxidative stress in intraocular pressure (IOP) elevation and optic nerve damage may be involved in the pathogenesis of glaucoma. We reported previously that a lower level of systemic antioxidative capacity is associated with IOP elevation in open-angle glaucoma (OAG). We assessed the correlation between the visual field sensitivity value, i.e., mean deviation (MD) and systemic levels of prooxidants and antioxidants by analyzing the blood biochemistry in 202 patients with glaucoma. Serum levels of lipid peroxides, ferric-reducing activity and thiol antioxidant activity were measured using the diacron reactive oxygen metabolite (dROM), biological antioxidant potential (BAP) and sulfhydryl (SH) tests, respectively, using a free-radical analyzer. Univariate and multivariate analyses suggested a positive correlation between MD and BAP (R = 0.005 and P = 0.0442 by a multiple regression model adjusted for seven demographic parameters), but no significant associations between the MD and the dROM (R = 0.002 and P = 0.8556) and SH tests (R = −0.001 and P = 0.8280). Use of more antiglaucoma medication and primary OAG rather than normal tension glaucoma also were associated significantly with worse visual field damage. This large and comprehensive assessment of the association between systemic redox status and visual field damage in OAG suggests that lower systemic antioxidant capacity measured by ferric-reducing activity is associated with more severe visual field damage in OAG that partly explained its roles in IOP elevation.
Increased olfactory bulb acetylcholine bi-directionally modulates glomerular odor sensitivity
Authors: Bendahmane Mounir , Ogg M. Cameron , Ennis Matthew , Fletcher Max L. 
Published: May 2016

AbstractThe glomerular layer of the olfactory bulb (OB) receives heavy cholinergic input from the horizontal limb of the diagonal band of Broca (HDB) and expresses both muscarinic and nicotinic acetylcholine (ACh) receptors. However, the effects of ACh on OB glomerular odor responses remain unknown. Using calcium imaging in transgenic mice expressing the calcium indicator GCaMP2 in the mitral/tufted cells, we investigated the effect of ACh on the glomerular responses to increasing odor concentrations. Using HDB electrical stimulation and in vivo pharmacology, we find that increased OB ACh leads to dynamic, activity-dependent bi-directional modulation of glomerular odor response due to the combinatorial effects of both muscarinic and nicotinic activation. Using pharmacological manipulation to reveal the individual receptor type contributions, we find that m2 muscarinic receptor activation increases glomerular sensitivity to weak odor input whereas nicotinic receptor activation decreases sensitivity to strong input. Overall, we found that ACh in the OB increases glomerular sensitivity to odors and decreases activation thresholds. This effect, along with the decreased responses to strong odor input, reduces the response intensity range of individual glomeruli to increasing concentration making them more similar across the entire concentration range. As a result, odor representations are more similar as concentration increases.