bluuebunny/crossref_metadata_2025_split huggingface dataset:
The brown and brite adipocyte marker Cox7a1 is not required for non-shivering thermogenesis in mice
Authors: Maurer Stefanie F. , Fromme Tobias , Grossman Lawrence I. , Hüttemann Maik , Klingenspor Martin 
Published: December 2015

AbstractThe cytochrome c oxidase subunit isoform Cox7a1 is highly abundant in skeletal muscle and heart and influences enzyme activity in these tissues characterised by high oxidative capacity. We identified Cox7a1, well-known as brown adipocyte marker gene, as a cold-responsive protein of brown adipose tissue. We hypothesised a mechanistic relationship between cytochrome c oxidase activity and Cox7a1 protein levels affecting the oxidative capacity of brown adipose tissue and thus non-shivering thermogenesis. We subjected wildtype and Cox7a1 knockout mice to different temperature regimens and tested characteristics of brown adipose tissue activation. Cytochrome c oxidase activity, uncoupling protein 1 expression and maximal norepinephrine-induced heat production were gradually increased during cold-acclimation, but unaffected by Cox7a1 knockout. Moreover, the abundance of uncoupling protein 1 competent brite cells in white adipose tissue was not influenced by presence or absence of Cox7a1. Skin temperature in the interscapular region of neonates was lower in uncoupling protein 1 knockout pups employed as a positive control, but not in Cox7a1 knockout pups. Body mass gain and glucose tolerance did not differ between wildtype and Cox7a1 knockout mice fed with high fat or control diet. We conclude that brown adipose tissue function in mice does not require the presence of Cox7a1.
The Assembling of Poly (3-Octyl-Thiophene) on CVD Grown Single Layer Graphene
Authors: Jiang Yanqiu , Yang Ling , Guo Zongxia , Lei Shengbin 
Published: December 2015

AbstractThe interface between organic semiconductor and graphene electrode, especially the structure of the first few molecular layers at the interface, is crucial for the device properties such as the charge transport in organic field effect transistors. In this work, we have used scanning tunneling microscopy to investigate the poly (3-octyl-thiophene) (P3OT)-graphene interface. Our results reveal the dynamic assembling of P3OT on single layer graphene. As on other substrates the epitaxial effect plays a role in determining the orientation of the P3OT assembling, however, the inter-thiophene distance along the backbone is consistent with that optimized in vaccum, no compression was observed. Adsorption of P3OT on ripples is weaker due to local curvature, which has been verified both by scanning tunneling microscopy and density functional theory simulation. Scanning tunneling microscopy also reveals that P3OT tends to form hairpin folds when meets a ripple.
Enzyme-guided DNA Sewing Architecture
Authors: Song In Hyun , Shin Seung Won , Park Kyung Soo , Lansac Yves , Jang Yun Hee , Um Soong Ho 
Published: December 2015

AbstractWith the advent of nanotechnology, a variety of nanoarchitectures with varied physicochemical properties have been designed. Owing to the unique characteristics, DNAs have been used as a functional building block for novel nanoarchitecture. In particular, a self-assembly of long DNA molecules via a piece DNA staple has been utilized to attain such constructs. However, it needs many talented prerequisites (e.g., complicated computer program) with fewer yields of products. In addition, it has many limitations to overcome: for instance, (i) thermal instability under moderate environments and (ii) restraint in size caused by the restricted length of scaffold strands. Alternatively, the enzymatic sewing linkage of short DNA blocks is simply designed into long DNA assemblies but it is more error-prone due to the undeveloped sequence data. Here, we present, for the first time, a comprehensive study for directly combining DNA structures into higher DNA sewing constructs through the 5′-end cohesive ligation of T4 enzyme. Inspired by these achievements, the synthesized DNA nanomaterials were also utilized for effective detection and real-time diagnosis of cancer-specific and cytosolic RNA markers. This generalized protocol for generic DNA sewing is expected to be useful in several DNA nanotechnology as well as any nucleic acid-related fields.
Tunable lasing in cholesteric liquid crystal elastomers with accurate measurements of strain
Authors: Varanytsia Andrii , Nagai Hama , Urayama Kenji , Palffy-Muhoray Peter 
Published: December 2015

AbstractWe report wide range and reversible tuning of the selective reflection band of a single crystal cholesteric liquid crystal elastomer (CLCE). The tuning is the result of mechanical shortening of the helical pitch achieved by imposing a uniform uniaxial strain along the helical axis. On doping the CLCE sample with a laser dye, we observe lasing from the CLCE in both glassy and rubbery states. By changing the cholesteric pitch, mechanical compression provides tuning of the laser emission from the dye doped CLCE over a significant part of the fluorescence band of the laser dye. In this work we demonstrate for the first time that both the CLCE pitch and the lasing wavelength are linearly dependent on the strain imposed on the CLCE film.
Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation
Authors: Tan Ya-Jing , Zhang Xue-Ying , Ding Guo-Lian , Li Rong , Wang Li , Jin Li , Lin Xian-Hua , Gao Ling , Sheng Jian-Zhong , Huang He-Feng 
Published: December 2015

AbstractHyperosmotic stress may induce apoptosis of different cells. However, oocytes show tolerance to osmotic stress during cryopreservation by vitrification, which is an assisted reproductive technique. The underlying mechanism is still not understood. Here, we demonstrated that hyperosmosis produced by high concentrations of cryoprotectants, including DMSO, ethylene glycol and sucrose, significantly upregulated the protein levels of aquaporin (AQP) 7, but not AQP3 and AQP9, in mouse oocytes. Knockdown of AQP7 expression by siRNA-injection significantly reduced the survival of oocytes after vitrification. In oocytes, AQP7 was shown to bind with F-actin, a protein involved in almost all biological events. Moreover, we found that hyperosmosis could upregulate the phosphorylation levels of CPE-binding protein (CPEB) and Aurora A. Inhibition of the PI3K and PKC pathways blocked the hyperosmosis-induced upregulation of AQP7 and the phosphorylation of CPEB and Aurora A in oocytes. In conclusion, hyperosmosis could upregulate the expression of AQP7 via Aurora A/CPEB phosphorylation mediated by the PI3K and PKC pathways and upregulation of AQP7 plays an important role in improving of tolerance to hyperosmotic stress and survival of oocytes during cryopreservation by vitrification.
CB1 cannabinoid receptor enrichment in the ependymal region of the adult human spinal cord
Authors: Paniagua-Torija Beatriz , Arevalo-Martin Angel , Ferrer Isidro , Molina-Holgado Eduardo , Garcia-Ovejero Daniel 
Published: December 2015

AbstractCannabinoids are involved in the regulation of neural stem cell biology and their receptors are expressed in the neurogenic niches of adult rodents. In the spinal cord of rats and mice, neural stem cells can be found in the ependymal region, surrounding the central canal, but there is evidence that this region is largely different in adult humans: lacks a patent canal and presents perivascular pseudorosettes, typically found in low grade ependymomas. Using Laser Capture Microdissection, Taqman gene expression assays and immunohistochemistry, we have studied the expression of endocannabinoid system components (receptors and enzymes) at the human spinal cord ependymal region. We observe that ependymal region is enriched in CB1 cannabinoid receptor, due to high CB1 expression in GFAP+ astrocytic domains. However, in human spinal cord levels that retain central canal patency we found ependymal cells with high CB1 expression, equivalent to the CB1HIGH cell subpopulation described in rodents. Our results support the existence of ependymal CB1HIGH cells across species and may encourage further studies on this subpopulation, although only in cases when central canal is patent. In the adult human ependyma, which usually shows central canal absence, CB1 may play a different role by modulating astrocyte functions.
Electrolithography- A New and Versatile Process for Nano Patterning
Authors: Talukder Santanu , Kumar Praveen , Pratap Rudra 
Published: December 2015

AbstractWe report a new lithography technique based on electromigration driven material transport for drawing patterns at nanometer scales in ambient conditions. We use a thin metal film as a masking layer and a polymer layer beneath it as a pattern transfer layer. The desired pattern is drawn in the metal layer by etching the metal with a conducting scanning probe assisted by liquid electromigration. The pattern drawn on the metal layer is transferred to the polymer layer by etching the polymer with an appropriate solvent. Subsequently, the pattern is transferred to the desired material layer using a film deposition technique followed by conventional lift-off process. Using this simple technique, we have achieved pattern resolutions of 9 nm on the polymer and 40 nm on transferring the pattern to another material. Based on the ease of use and process costs, this technique promises to be competitive to e-beam lithography that employs high energy and ultra-high vacuum, or the industrial standard ultra-violet light photolithography that employs extremely expensive implements to reach nano-scale resolutions. We also demonstrate direct mask writing using this technique and explain the fundamentals behind the workings of the developed method.
Surgical Ventricular Entry is a Key Risk Factor for Leptomeningeal Metastasis of High Grade Gliomas
Authors: Roelz Roland , Reinacher Peter , Jabbarli Ramazan , Kraeutle Rainer , Hippchen Beate , Egger Karl , Weyerbrock Astrid , Machein Marcia 
Published: December 2015

AbstractLeptomeningeal metastasis (LM) of high grade gliomas (HGG) can lead to devastating disease courses. Understanding of risk factors for LM is important to identify patients at risk. We reviewed patient records and magnetic resonance imaging (MRI) of all patients with a first diagnosis of HGG who underwent surgery in our institution between 2008 and 2012. To assess the influence of potential risk factors for LM and the impact of LM on survival multivariate statistics were performed. 239 patients with a diagnosis of HGG and at least 6 months of MRI and clinical follow-up were included. LM occurred in 27 (11%) patients and was symptomatic in 17 (65%). A strong correlation of surgical entry to the ventricle and LM was found (HR: 8.1). Ventricular entry was documented in 137 patients (57%) and LM ensued in 25 (18%) of these. Only two (2%) of 102 patients without ventricular entry developed LM. Median overall survival of patients after diagnosis of LM (239 days) was significantly shorter compared to patients without LM (626 days). LM is a frequent complication in the course of disease of HGG and is associated with poor survival. Surgical entry to the ventricle is a key risk factor for LM.
Defining the Schistosoma haematobium kinome enables the prediction of essential kinases as anti-schistosome drug targets
Authors: Stroehlein Andreas J. , Young Neil D. , Jex Aaron R. , Sternberg Paul W. , Tan Patrick , Boag Peter R. , Hofmann Andreas , Gasser Robin B. 
Published: December 2015

AbstractThe blood fluke Schistosoma haematobium causes urogenital schistosomiasis, a neglected tropical disease (NTD) that affects more than 110 million people. Treating this disease by targeted or mass administration with a single chemical, praziquantel, carries the risk that drug resistance will develop in this pathogen. Therefore, there is an imperative to search for new drug targets in S. haematobium and other schistosomes. In this regard, protein kinases have potential, given their essential roles in biological processes and as targets for drugs already approved by the US Food and Drug Administration (FDA) for use in humans. In this context, we defined here the kinome of S. haematobium using a refined bioinformatic pipeline. We classified, curated and annotated predicted kinases and assessed the developmental transcription profiles of kinase genes. Then, we prioritised a panel of kinases as potential drug targets and inferred chemicals that bind to them using an integrated bioinformatic pipeline. Most kinases of S. haematobium are very similar to those of its congener, S. mansoni, offering the prospect of designing chemicals that kill both species. Overall, this study provides a global insight into the kinome of S. haematobium and should assist the repurposing or discovery of drugs against schistosomiasis.
Distant Influence of Kuroshio Eddies on North Pacific Weather Patterns?
Authors: Ma Xiaohui , Chang Ping , Saravanan R. , Montuoro Raffaele , Hsieh Jen-Shan , Wu Dexing , Lin Xiaopei , Wu Lixin , Jing Zhao 
Published: December 2015

AbstractHigh-resolution satellite measurements of surface winds and sea-surface temperature (SST) reveal strong coupling between meso-scale ocean eddies and near-surface atmospheric flow over eddy-rich oceanic regions, such as the Kuroshio and Gulf Stream, highlighting the importance of meso-scale oceanic features in forcing the atmospheric planetary boundary layer (PBL). Here, we present high-resolution regional climate modeling results, supported by observational analyses, demonstrating that meso-scale SST variability, largely confined in the Kuroshio-Oyashio confluence region (KOCR), can further exert a significant distant influence on winter rainfall variability along the U.S. Northern Pacific coast. The presence of meso-scale SST anomalies enhances the diabatic conversion of latent heat energy to transient eddy energy, intensifying winter cyclogenesis via moist baroclinic instability, which in turn leads to an equivalent barotropic downstream anticyclone anomaly with reduced rainfall. The finding points to the potential of improving forecasts of extratropical winter cyclones and storm systems and projections of their response to future climate change, which are known to have major social and economic impacts, by improving the representation of ocean eddy–atmosphere interaction in forecast and climate models.
Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis
Authors: Wang Qian , Du Jianjun , Yu Pengfei , Bai Bin , Zhao Zhanwei , Wang Shiqi , Zhu Junjie , Feng Quanxin , Gao Yun , Zhao Qingchuan , Liu Chaoxu 
Published: December 2015

AbstractHepatic steatosis (HS) can exacerbate acute pancreatitis (AP). This study aimed to investigate the relation between α1-antitrypsin (AAT) and acute pancreatitis when patients have HS. Using proteomic profiling, we identified 18 differently expressed proteins pots in the serum of rats with or without HS after surgical establishment of AP. AAT was found to be one of the significantly down-regulated proteins. AAT levels were significantly lower in hepatic steatosis acute pancreatitis (HSAP) than in non-HSAP (NHSAP) (P < 0.001). To explore the clinical significance of these observations, we measured the levels of AAT in the serum of 240 patients with HSAP, NHSAP, fatty liver disease (FLD), or no disease. Compared with healthy controls, serum AAT levels in patients with NHSAP were significantly higher (P < 0.01), while in patients with HSAP serum AAT levels were significantly lower (P < 0.01). Further studies showed that acute physiology and chronic health evaluation (APACHE-II) scores were negatively correlated with serum AAT levels (r = −0.85, P < 0.01). In conclusion, low serum levels of AAT in patients with HSAP are correlated with disease severity and AAT may represent a potential target for therapies aiming to improve pancreatitis.
Protein kinase Cζ exhibits constitutive phosphorylation and phosphatidylinositol-3,4,5-triphosphate-independent regulation
Authors: Tobias Irene S. , Kaulich Manuel , Kim Peter K. , Simon Nitya , Jacinto Estela , Dowdy Steven F. , King Charles C. , Newton Alexandra C. 
Published: December 2015

Atypical protein kinase C (aPKC) isoenzymes are key modulators of insulin signalling, and their dysfunction correlates with insulin-resistant states in both mice and humans. Despite the engaged interest in the importance of aPKCs to type 2 diabetes, much less is known about the molecular mechanisms that govern their cellular functions than for the conventional and novel PKC isoenzymes and the functionally-related protein kinase B (Akt) family of kinases. Here we show that aPKC is constitutively phosphorylated and, using a genetically-encoded reporter for PKC activity, basally active in cells. Specifically, we show that phosphorylation at two key regulatory sites, the activation loop and turn motif, of the aPKC PKCζ in multiple cultured cell types is constitutive and independently regulated by separate kinases: ribosome-associated mammalian target of rapamycin complex 2 (mTORC2) mediates co-translational phosphorylation of the turn motif, followed by phosphorylation at the activation loop by phosphoinositide-dependent kinase-1 (PDK1). Live cell imaging reveals that global aPKC activity is constitutive and insulin unresponsive, in marked contrast to the insulin-dependent activation of Akt monitored by an Akt-specific reporter. Nor does forced recruitment to phosphoinositides by fusing the pleckstrin homology (PH) domain of Akt to the kinase domain of PKCζ alter either the phosphorylation or activity of PKCζ. Thus, insulin stimulation does not activate PKCζ through the canonical phosphatidylinositol-3,4,5-triphosphate-mediated pathway that activates Akt, contrasting with previous literature on PKCζ activation. These studies support a model wherein an alternative mechanism regulates PKCζ-mediated insulin signalling that does not utilize conventional activation via agonist-evoked phosphorylation at the activation loop. Rather, we propose that scaffolding near substrates drives the function of PKCζ.
Circulating FGF21 proteolytic processing mediated by fibroblast activation protein
Authors: Zhen Eugene Y. , Jin Zhaoyan , Ackermann Bradley L. , Thomas Melissa K. , Gutierrez Jesus A. 
Published: December 2015

Fibroblast growth factor 21 (FGF21), a hormone implicated in the regulation of glucose homoeostasis, insulin sensitivity, lipid metabolism and body weight, is considered to be a promising therapeutic target for the treatment of metabolic disorders. Despite observations that FGF21 is rapidly proteolysed in circulation rending it potentially inactive, little is known regarding mechanisms by which FGF21 protein levels are regulated. We systematically investigated human FGF21 protein processing using mass spectrometry. In agreement with previous reports, circulating human FGF21 was found to be cleaved primarily after three proline residues at positions 2, 4 and 171. The extent of FGF21 processing was quantified in a small cohort of healthy human volunteers. Relative abundance of FGF21 proteins cleaved after Pro-2, Pro-4 and Pro-171 ranged from 16 to 30%, 10 to 25% and 10 to 34%, respectively. Dipeptidyl peptidase IV (DPP-IV) was found to be the primary protease responsible for N-terminal cleavages after residues Pro-2 and Pro-4. Importantly, fibroblast activation protein (FAP) was implicated as the protease responsible for C-terminal cleavage after Pro-171, rendering the protein inactive. The requirement of FAP for FGF21 proteolysis at the C-terminus was independently demonstrated by in vitro digestion, immunodepletion of FAP in human plasma, administration of an FAP-specific inhibitor and by human FGF21 protein processing patterns in FAP knockout mouse plasma. The discovery that FAP is responsible for FGF21 inactivation extends the FGF21 signalling pathway and may enable novel approaches to augment FGF21 actions for therapeutic applications.
Millian Liberalism and Colonial Oppression
Authors: Brown D.G. 
Published: December 2015

In nineteenth-century Europe …. [w]ith rare exceptions liberals approved of colonialism and provided it with a legitimizing ideology …. Liberalism became missionary, ethnocentric, and narrow, dismissing non-liberal ways of life and thought as primitive and in need of the liberal civilizing mission.This is the judgement passed by Professor Bhikhu Parekh in his 1994 essay “Decolonizing Liberalism.” His deference to John Stuart Mill is shown in his making Mill not one of the exceptions, but rather the central object of attack. It would seem indeed that if the charges can be made good against Mill they will hold against nineteenth-century liberalism in general, and perhaps in some degree against twenty-first-century liberalism.Simple piety moves me to offer some defence of Mill's own good judgement, particularly in relation to India. But, in dealing with a phenomenon like liberalism, we need always to maintain a distinction which tends to blur. Doctrines and assertions are one thing. Historical movements and trends in a society are another.
Justice Theory and Oppression
Authors: Harvey J. 
Published: December 2015

Contending views of social justice often confront each other over claims about basic rights. In this paper I will begin with a familiar dispute of this kind, the crux of which proves to be the distribution of economic benefits and burdens. I will argue that it is is indeed illuminating to consider basic rights, and important in particular to examine an especially fundamental set of rights, namely, those that attach to the moral relationships that define the moral community. The blocking of these rights is a moral concern in itself and also often underlies more obvious social and economic inequalities. By considering this set of rights, we can uncover an unsuspected kind of justice which makes moral demands independently of a concern for distributive justice.There is a longstanding and influential tradition of claiming that the duties of justice are solely “negative” duties. On this view the concerns of justice are concerns about “negative rights” of an especially basic kind, usually subsumed under the heading of “individual freedom.” Justice consists in our “not interfering with” these basic negative rights.
Epistemic Oppression and Epistemic Privilege
Authors: Fricker Miranda 
Published: December 2015

[T]he dominated live in a world structured by others for their purposes — purposes that at the very least are not our own and that are in various degrees inimical to our development and even existence.We are perhaps used to the idea that there are various species of oppression: political, economic, or sexual, for instance. But where there is the phenomenon that Nancy Hartsock picks out in saying that the world is “structured” by the powerful to the detriment of the powerless, there is another species of oppression at work, one that has not been registered in mainstream epistemology: epistemic oppression. The word ‘structured’ may be read materially, so as to imply that social institutions and practices favour the powerful, or ontologically, so as to imply that the powerful somehow constitute the world. But for present purposes I am interested only in an epistemological reading, which implies that the powerful have some sort of unfair advantage in “structuring” our understandings of the social world. I will try to present an account of what this initially vague idea involves. I hope thereby to explain an exact sense in which the powerful can have a kind of epistemic advantage that means the powerless are epistemically oppressed.