bluuebunny/crossref_metadata_2025_split huggingface dataset:
MDA‐MB‐231 and 8701BC breast cancer lines promote the migration and invasiveness of ECV304 cells on 2D and 3D type‐I collagen matrix
Authors: Saladino Silvia , Salamone Monica , Ghersi Giulio 
Published: July 2017

AbstractTumor angiogenesis is a multiphasic process, having the extracellular matrix remodeling as critical step. Different classes of proteolytic enzymes in matrix digestion/remodeling are involved. The role of lytic enzymes and their activation mode have not been completely elucidated. Herein, the crosstalk between endothelia and tumor cells, by realization of bi‐ and three‐dimensional endothelial and breast cancer cells co‐cultures, were studied in vitro. Particularly, the effects of two tumor conditioned media (TCM) were assessed about endothelial proliferation, migration, and invasiveness. An increase in expression of pro‐MMP9 was detected when endothelial cells were cultured in the presence of both TCM; such as an up‐regulation of MMP1 and MMP14 and a down‐regulation of MMP7. Moreover the increased MMP2 gene expression from one of them and the stimulation MMP3 synthesis from the other one were observed; an increases of β3‐integrin, VEGFA, and DPP4 molecules were detected when endothelia cells are cultured with both TCM. The selection/characterization of elements present in conditioned media from breast cancer cells differently affect endothelial cells, make them potential effectors useful in breast cancer treatment.
Evaluating the impact of the London Pathway Project
Authors: Jolliffe Darrick , Cattell Jack , Raza Annabelle , Minoudis Philip 
Published: July 2017

AbstractBackgroundThe London Pathway Project (LPP) is an innovative whole‐systems approach to addressing the needs of offenders who have severe personality disorder, with the goal of reducing their risk of harm. Previous research has evaluated the initial implementation of the LPP.AimsThis paper focused on evaluating the impact of the LPP on a number of criminogenic needs over time and its impact on the risk of reoffending and harm compared with a similar group who did not experience the pathway.MethodData for men who had been identified for the LPP were used to explore changes in key criminogenic needs an average of 11 months after commencing on the pathway. In addition, Offender Assessment System data was used to match men who had experienced the LPP for at least 12 months to a comparison group on key demographic and criminal history variables. Changes in validated risk assessment devices and changes in practitioners' perception of risk were examined.ResultsThe LPP was associated with desirable within‐individual change for most of the criminogenic needs explored. However, strong non‐desirable changes in lifestyle and associates were also identified, but this was particularly the case for those sentenced to prison. When compared with a matched group, those identified for the pathway showed a significant reduction on an objective measure of risk of reoffending but were rated as having significantly increased risk of harm on the basis of practitioner's perceptions. There was no evidence that greater progression along the pathway was associated with greater benefits.ConclusionsThis is the first impact evaluation of the LPP, and the results were generally positive in terms of its relation to criminogenic needs and risks. Much more research that clearly links project inputs to actual behavioural outcomes, such as later reoffending, is needed.ImplicationsThis initial evaluation of the impact of the LPP could be used as baseline data to examine the impact of the pathway over time, and with greater precision (e.g. matching on personality features). Copyright © 2017 John Wiley & Sons, Ltd.
S, N Dual‐Doped Graphene Fibers: A High‐Performance Electrode Material for Supercapacitors
Authors: Kan Yanfang , Ma Xinlong , Ning Guoqing , Li Yongfeng , Zhou Yasong 
Published: July 2017

AbstractSulfur and nitrogen dual‐doped graphene fibers (SNGFs) were prepared through an optimized chemical vapor deposition. The SNGFs exhibit a stable 3D fibrous structure, high surface area, abundant mesopores, uniform distribution of S and N atoms and more defects. As electrodes for supercapacitors, the specific capacitance of SNGFs reaches 311 F g−1. The SNGF//SNGF symmetric supercapacitor delivers maximum energy and power densities of 37.7 W h kg−1 and 14 kW kg−1, with excellent cycling stability (92.2 % capacitance remained after 5000 cycles). The result indicates that the SNGFs are a promising candidate as a high‐quality electrode material for supercapacitors.
Measurement of Drug‐Stabilized Topoisomerase II Cleavage Complexes by Flow Cytometry
Authors: de Campos Nebel Marcelo , Palmitelli Micaela , González‐Cid Marcela 
Published: July 2017

AbstractThe poisoning of Topoisomerase II (Top2) has been found to be useful as a therapeutic strategy for the treatment of several tumors. The mechanism of Top2 poisons involves a drug‐mediated stabilization of a Top2‐DNA complex, termed Top2 cleavage complex (Top2cc), which maintains a 5′ end of DNA covalently bound to a tyrosine from Top2 through a phosphodiester group. Drug‐stabilized Top2cc leads to Top2‐linked‐DNA breaks, which are believed to mediate their cytotoxicity. Several time‐consuming or cell type‐limiting assays have been used in the past to study drug‐stabilized Top2cc. Here, we describe a flow cytometry‐based method that allows a rapid assessment of drug‐induced Top2cc, which is suitable for high throughput analysis in almost any kind of human cell. The analyses of the drug‐induced Top2cc in the cell cycle context and the possibility to track its removal are additional benefits from this methodology. © 2017 by John Wiley & Sons, Inc.
CyTOF Mass Cytometry for Click Proliferation Assays
Authors: Tosevski Vinko , Ulashchik Egor , Trovato Andrea , Cappella Paolo 
Published: July 2017

AbstractNovel cell analyzers, including polychromatic flow cytometers and isotopical cytometry by time of flight (CyTOF) mass cytometers, enable simultaneous measurement of virtually bondless characteristics at the single‐cell level. BrdU assays for quantifying cellular proliferation are common but have several limitations, including the need for a DNA denaturation step and inability to simultaneously resolve multiple parameters and phenotypic complexity. Click chemistry reactions have become popular in the past decade, as they can resolve these issues. This protocol introduces a novel assay able to bridge flow cytometry and CyTOF analysis for active S‐phase determination in cell cycle applications, combining well‐established click chemistry with a novel iodo‐deoxyuridine (IdU) azide derivative and a cross‐reactive anti‐IdU antibody for detecting incorporated EdU during DNA synthesis. This method is preferred over traditional BrdU‐based assays for complex and multiparametric experiments. It provides a feasible cost‐effective approach for detecting ethynyl‐labeled nucleotides, with the advantage of combining flow and mass cytometry analyses. © 2017 by John Wiley & Sons, Inc.
Animal Model of Chronic Migraine‐Associated Pain
Authors: Moye Laura S. , Pradhan Amynah A. A. 
Published: July 2017

AbstractMigraine is a debilitating condition that affects hundreds of millions of people worldwide. A subset of these patients experience chronic migraine, resulting in long‐term disability and a severely lowered quality of life. The development of novel migraine therapies has been slow, partially due to the small number of predictive animal models. We have recently developed a novel model of chronic migraine‐associated pain, using the known human migraine trigger, nitroglycerin. Injection of nitroglycerin evokes an acute mechanical hyperalgesia, which is sensitive to the acute migraine therapy sumatriptan. In addition, chronic administration of nitroglycerin produces a progressive and sustained decrease in basal mechanical responses, and this hypersensitivity is blocked by migraine preventatives such as topiramate. This mouse model of chronic migraine can be used to study the mechanisms underlying progression of migraine from an episodic to a chronic disorder, and for identifying and screening novel acute and preventive migraine therapies. © 2017 by John Wiley & Sons, Inc.
Incorporating thresholds into understanding salinity tolerance: A study using salt‐tolerant plants in salt marshes
Authors: He Qiang , Silliman Brian R. , Cui Baoshan 
Published: July 2017

AbstractAlthough salinity in many ecosystems such as salt marshes can be extremely high, an asymmetry in salinity range between experimental studies (relatively narrow) and field conditions (potentially broad) has strongly affected current understanding of plant salinity tolerance. To improve understanding, it is thus important to examine plant tolerances over a broad range of salinities and identify potential tolerance thresholds. We examine tolerances of two widely distributed marsh plants, Suaeda salsa and Salicornia europaea, to salinities ranging from 0 to 100 g/kg, and determine survival, above‐ and belowground biomass after 8 weeks of salinity treatment. Both species, Sa. europaea in particular, have much broader salinity tolerances than other plants previously examined, (2) plant survival, above‐ and belowground biomass have remarkably different responses to salinity, and (3) there is a nonlinear, threshold response of S. salsa to salinity, above which S. salsa survivorship drastically decreases. These results provide multiple important insights. Our study suggests that the potential for using these halophytes to revegetate and restore salt‐affected land may be greater than previously thought, and highlights the importance of studying multiple plant responses. Importantly, our study calls for a better integration of thresholds into understanding plant salinity tolerances and their applications.
Contrast‐enhanced computed tomography with myocardial three‐dimensional printing can guide treatment in symptomatic hypertrophic obstructive cardiomyopathy
Authors: Hamatani Yasuhiro , Amaki Makoto , Kanzaki Hideaki , Yamashita Kizuku , Nakashima Yasuteru , Shibata Atsushi , Okada Atsushi , Takahama Hiroyuki , Hasegawa Takuya , Shimahara Yusuke , Sugano Yasuo , Fujita Tomoyuki , Shiraishi Isao , Yasuda Satoshi , Kobayashi Junjiro , Anzai Toshihisa 
Published: July 2017

AbstractBoth surgical myectomy and percutaneous transluminal septal myocardial ablation are effective treatments for drug‐refractory symptomatic hypertrophic obstructive cardiomyopathy (HOCM). However, in some cases, it is not easy to elucidate the abnormal structure of left ventricular outflow obstruction to adopt these treatments. Here, we presented a young female patient with drug‐refractory symptomatic HOCM. In this case, contrast‐enhanced computed tomography enabled us to assess the suitability of percutaneous transluminal septal myocardial ablation. By creating three‐dimensional printed models using computed tomography data, we could also visualize intracardiac structure and simulate the surgical procedure. A multimodality assessment strategy is useful for evaluating patients complicated with drug‐refractory symptomatic HOCM.
A Phase 2a dose‐escalation study of the safety, tolerability, pharmacokinetics and haemodynamic effects of BMS‐986231 in hospitalized patients with heart failure with reduced ejection fraction
Authors: Tita Cristina , Gilbert Edward M. , Van Bakel Adrian B. , Grzybowski Jacek , Haas Garrie J. , Jarrah Mohammad , Dunlap Stephanie H. , Gottlieb Stephen S. , Klapholz Marc , Patel Parag C. , Pfister Roman , Seidler Tim , Shah Keyur B. , Zieliński Tomasz , Venuti Robert P. , Cowart Douglas , Foo Shi Yin , Vishnevsky Alexander , Mitrovic Veselin 
Published: July 2017

AbstractAimsThis study was designed to evaluate the safety, tolerability and haemodynamic effects of BMS‐986231, a novel second‐generation nitroxyl donor with potential inotropic, lusitropic and vasodilatory effects in patients hospitalized with decompensated heart failure and reduced ejection fraction (HFrEF).Methods and resultsForty‐six patients hospitalized with decompensated HFrEF were enrolled into four sequential dose‐escalation cohorts in this double‐blind, randomized, placebo‐controlled Phase 2a study. Patients with baseline pulmonary capillary wedge pressure (PCWP) of ≥20 mmHg and a cardiac index of ≤2.5 L/min/m2 received one 6‐h i.v. infusion of BMS‐986231 (at 3, 5, 7 or 12 µg/kg/min) or placebo. BMS‐986231 produced rapid and sustained reductions in PCWP, as well as consistent reductions in time‐averaged pulmonary arterial systolic pressure, pulmonary arterial diastolic pressure and right atrial pressure. BMS‐986231 increased non‐invasively measured time‐averaged stroke volume index, cardiac index and cardiac power index values, and decreased total peripheral vascular resistance. There was no evidence of increased heart rate, drug‐related arrhythmia or symptomatic hypotension with BMS‐986231. Analyses of adverse events throughout the 30‐day follow‐up did not identify any toxicities specific to BMS‐986231, with the potential exception of infrequent mild‐to‐moderate headaches during infusion. There were no treatment‐related serious adverse events.ConclusionsBMS‐986231 demonstrated a favourable safety and haemodynamic profile in patients hospitalized with advanced heart failure. Based on preclinical data and these study's findings, it is possible that the haemodynamic benefits may be mediated by inotropic and/or lusitropic as well as vasodilatory effects. The therapeutic potential of BMS‐986231 should be further assessed in patients with heart failure.
Physicochemical characteristics of pristine and functionalized graphene
Authors: Bourdo Shawn E. , Al Faouri Radwan , Sleezer Robert , Nima Zeid A. , Lafont Andersen , Chhetri Bijay P. , Benamara Mourad , Martin Betty , Salamo Gregory J. , Biris Alexandru S. 
Published: July 2017

AbstractGraphene‐based nanomaterials have received significant attention in the last decade due to their interesting properties. Its electrical and thermal conductivity and strength make graphene well suited for a variety of applications, particularly for use as a composite material in plastics. Furthermore, much work is taking place to utilize graphene as a biomaterial for uses such as drug delivery and tissue regeneration scaffolds. Owing to the rapid progress of graphene and its potential in many marketplaces, the potential toxicity of these materials has garnered attention. Graphene, while simple in its purest form, can have many different chemical and physical properties. In this paper, we describe our toxicity evaluation of pristine graphene and a functionalized graphene sample that has been oxidized for enhanced hydrophilicity, which was synthesized from the pristine sample. The samples were characterized by X‐ray photoelectron spectroscopy, Raman spectroscopy, infrared spectroscopy, thermogravimetric analysis, zeta‐potential, atomic force microscopy and electron microscopy. We discuss the disagreement between the size of imaged samples analyzed by atomic force microscopy and by transmission electron microscopy. Furthermore, the samples each exhibit quite different surface chemistry and structure, which directly affects their interaction with aqueous environments and is important to consider when evaluating the toxicity of materials both in vitro and in vivo. Copyright © 2017 John Wiley & Sons, Ltd.
Notable Drug–Drug Interaction Between Etizolam and Itraconazole in Poor Metabolizers of Cytochrome P450 2C19
Authors: Yamamoto Takehito , Furihata Kenichi , Hisaka Akihiro , Moritoyo Takashi , Ogoe Kazuaki , Kusayama Shizuko , Motohashi Keiju , Mori Akiko , Iwatsubo Takeshi , Suzuki Hiroshi 
Published: July 2017

AbstractIn this study, impact of a polymorphism of CYP2C19 on drug–drug interaction (DDI) was examined for etizolam. The effect of itraconazole (a strong CYP3A inhibitor) on the pharmacokinetics of etizolam (a substrate of CYP2C19 and CYP3A) was assessed in both extensive metabolizers (EMs) and poor metabolizers (PMs) of CYP2C19. Sixteen participants (8 EMs and 8 PMs) received a single oral dose of etizolam (0.25 mg) on day 1. The participants ingested itraconazole (200 mg twice a day) on days 2–5. On day 5, participants received an oral dose of etizolam (0.25 mg) again. Before coadministration of itraconazole (day 1), the area under the time–plasma concentration curve from time zero to infinity (AUC∞) of etizolam was higher in PMs than in EMs (2.65‐fold, P < .01). Coadministration of itraconazole increased the AUC∞ of etizolam 1.66‐fold and 2.34‐fold in EMs and PMs, respectively (day 5). Consequently, AUC∞ was 6.18‐fold higher in PMs with itraconazole than that in EMs without itraconazole. The increase by itraconazole was larger in PMs (P < .01). In heterozygous EMs (hEMs), AUC∞ was simulated to be 2.56‐fold higher with itraconazole than that in EMs without itraconazole. We found that in vitro measurements of fraction metabolized (fm) using the liver microsome prepared from PM donors would be helpful to predict polymorphism‐dependent DDIs. These results suggest that the PMs and hEMs of a polymorphic CYP would be at higher risk of DDIs relative to EMs for drugs metabolized by both polymorphic and nonpolymorphic CYPs such as etizolam.
Temporal and spatial variability of surface‐based inversions over Europe based on ERA‐Interim reanalysis
Authors: Palarz Angelika , Celiński‐Mysław Daniel , Ustrnul Zbigniew 
Published: July 2017

ABSTRACTTropospheric temperature inversions seem to be an important feature of climate, as well as a significant factor affecting air quality and fog formation. The aim of this article is to investigate the temporal and spatial variability of surface‐based inversions (SBIs) over Europe. It is based on data derived from the ERA‐Interim reanalysis for the period 1981–2015. The study examines diurnal, seasonal and multiannual variability of temperature inversions based on their frequency, depth and strength. These three parameters are characterized by strong temporal variability – diurnal and seasonal, as well as strong spatial differentiation. It has been confirmed that the energy budget is the key factor responsible for diurnal, and partly seasonal variability of SBI occurrence. Its negative values lead to an intense cooling of active surface and initiate the formation of the radiative inversions, which are the most common type of inversion occurring over the mainland of Europe. Temperature inversions usually form shortly after dusk, increasing gradually their depth and strength until sunrise. At 0000 Universal Time Coordinated (UTC), SBI frequency attains higher values in the summer than in the winter over the predominant part of Europe. However, the inversion layers occurring then are noticeably shallower and weaker. Surface type strongly affects SBI properties. This is mostly marked in terms of the distinct differentiation between marine and land areas as well as the impact of vast glaciers located across Greenland and western part of Iceland. Moreover, the distribution of SBI depth and strength is shaped by atmospheric circulation. For instance, their higher values occur over Eastern Europe in the winter, which is associated with the influence of a seasonal high pressure system found over Russia. Taking into account multiannual variability, SBI parameters, primarily depth and strength, exhibit the most significant negative changes in the winter.
An air mass‐derived cool season climatology of synoptically forced Appalachian cold‐air damming
Authors: Ellis Andrew W. , Marston Michael L. , Nelson Daniel A. 
Published: July 2017

ABSTRACTAn air mass approach was used to identify episodes of cool season cold‐air damming (CAD) within the central Appalachian Mountains region of the eastern United States. Daily air mass type data were used to identify days on which moist polar (MP) air was regionally evident east of the mountains, while non‐MP air was in place at nearby stations west of the mountains. Over a 35‐year study period, 219 CAD days were identified (>6 per year) with the annual frequency exhibiting no trend but suggesting that El Niño (La Niña) coincides with a greater (lesser) frequency of CAD days in winter (December–February). Synoptic atmospheric composites reveal west‐to‐east migration of a parent anticyclone to a classic position along the border of the northeastern United States and southeastern Canada. This coincides with a pattern of amplifying and slowly eastward‐moving 500 hPa height anomalies characterized by positive (negative) values over eastern (western) North America that are signalled a few days in advance by the index representing the Pacific‐North American teleconnection pattern. Confinement of the CAD below the 850 hPa level is evident in the synoptic wind field, while the composite vertical profile of the atmosphere within the CAD environment further depicts the shallow nature of the surface‐based cool, moist air. Northeasterly winds at the surface veer to southeasterly within a few hundred metres above the surface, and then southwesterly at less than one km above the surface, at the 850 hPa level. The air mass approach to CAD identification appears to successfully identify regional occurrences of synoptically forced CAD, although it likely does not detect local and/or diabatically forced CAD.
The tissue residues of sodium dehydroacetate used as feed preservative in swine
Authors: Liu Hao , Han Lingling , Xie Jiayu , Wu Yingchao , Xie Yang , Zhang Yumei 
Published: July 2017

AbstractBACKGROUNDSodium dehydroacetate (Na‐DHA) is a food and feed additive with antimicrobial effects. There is little information on Na‐DHA residue levels in foods derived from animals. In this study, Na‐DHA residue levels in swine tissues were determined by HLPC, and the pharmacokinetics of Na‐DHA in tissues were determined.RESULTSThe Na‐DHA residue levels in swine tissues were <1.2 mg kg−1 at different withdrawal time after thirty‐two Duroc × Landrace × Yorkshire pigs were administered 200 mg Na‐DHA kg−1 through the feed for 30 days. In decreasing order of Na‐DHA residue levels, the tissues were kidney > liver > muscle > fat. The pharmacokinetics of Na‐DHA followed a binomial regression model, and the half‐time of Na‐DHA in swine tissues was 9.07 days for kidney, 7.19 days for liver, 6.66 days for muscle, and 5.39 days for fat tissue. The accuracy of the HPLC method for Na‐DHA determination ranged from 80.18% to 91.33% recovery, with coefficients of variation <6.4%, limit of detection of 0.08 mg kg−1, and limit of quantification of 0.2 mg kg−1.CONCLUSIONNa‐DHA included at 200 mg kg−1 in a swine diet is a safe feed additive based on residue elimination and ADI values reported. © 2017 Society of Chemical Industry
Rheology and microstructure of myofibrillar protein–olive oil composite gels: effect of different non‐meat protein as emulsifier
Authors: Wu Mangang , Fei Litian , Zhuang Tao , Lei Shumin , Ge Qingfeng , Yu Hai , Wang Jiahao , Wang Yaosong 
Published: July 2017

AbstractBACKGROUNDHeat‐induced composite gels were prepared with 20 g kg−1 (2%) myofibrillar protein (MP) sol and 100 g kg−1 (10%) olive oil pre‐emulsified by MP or non‐meat protein in 0.6 mol L−1 NaCl, at pH 6.2. The effect of different non‐meat protein (soy protein isolate, egg‐white protein isolate and sodium caseinate) pre‐emulsions on the rheological properties and microstructure of MP gel was evaluated.RESULTSAdding emulsion enhanced the gel strength of MP gel except for the soy protein isolate (SPI) as emulsifier group, but all emulsion group markedly improved (P < 0.05) the water‐holding capacity and the storage modulus (G′) of MP gels. SDS‐PAGE show that some non‐meat protein bands partially participated in the formation of MP composite gels with different kinds of emulsion added. Micrographs revealed that these emulsions made the gels become denser and more compact with subtle diverse effects.CONCLUSIONDifferent meat or non‐meat proteins as emulsifier have varied impacts on the rheology and microstructure of MP gels, indicating the potential and feasibility of these non‐meat proteins as emulsifiers to modify the textural properties in comminuted meat products. © 2017 Society of Chemical Industry