bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactiplantibacillus plantarum YY‐112 ameliorates mouse immunosuppression by enhancing B/T‐cell activation and maintaining Th1/Th2 homeostasis
Authors: Yang Ying , Luo Mengfan , Zhou Wanyi , Tao Wenyang , Li Jingrui , Xing Jianrong , Tan Quanqin , Guo Yuxing 
Published: October 2024

AbstractIn recent years, the development of immunosuppression has become common, and probiotics are a vital immunomodulatory strategy. This study evaluated the alleviating effect of Lactiplantibacillus plantarum (L. plantarum) YY‐112 on cyclophosphamide‐induced immune imbalance and resolved the possible mechanism of action. L. plantarum YY‐112 at 109 CFU/mL lowered loss of body weight and alleviated splenic injury, intestinal injury, and inflammation, according to the hematoxylin and eosin staining. Biochemical results showed that 109 CFU/mL L. plantarum YY‐112 increased interleukin‐10 and immunoglobulin M levels and decreased interferon‐γ levels. Additionally, the immunoregulatory effect at the molecular levels of L. plantarum YY‐112 was analyzed by transcriptome sequencing. L. plantarum YY‐112 significantly upregulated Cr2 and downregulated C5aR1 to regulate the complement system; downregulated S100a8, S100a9, and Mmp9 to inhibit neutrophil aggregation; upregulated Cd19, Cd72, Cd3e, Cd28, Cd80, Cd8a, and histocompatibility‐2‐related genes to regulate B‐ and T‐cell activation; and upregulated Xiap, Malt1, Ikbkb, Mapk11, Mef2c, Mapk12, Ras, and Myc to activate immune signaling pathways. Furthermore, L. plantarum YY‐112 improved intestinal microbial structure and reduced enrichment of Lachnospiraceae_NK4A136_group, norank_f_Lachnospiraceae, Desulfovibrio, Colidextribacter, and Helicobacter. In conclusion, these findings offer valuable insights into the potential mechanisms through which L. plantarum YY‐112 regulates immune imbalance.
Bacterial volatile organic compounds supplemented with light‐emitting diode lighting enhance antioxidant capacity and delay senescence in broccoli
Authors: Huang Taishan , Shi Junyan , Yue Xiaozhen , Yuan Shuzhi , Xu Xiangbin , Zuo Jinhua , Wang Qing 
Published: October 2024

AbstractBacterial volatile organic compounds (BVOCs) are biological volatiles that can have a positive effect on the postharvest preservation of fruits and vegetables. In the present study, fumigation of broccoli with BVOCs released by Lysinibacillus fusiformis supplemented with light‐emitting diode (LED) white light irradiation reduced the accumulation of reactive oxygen species in broccoli during senescence, enhanced the synthesis and accumulation of antioxidant substances, and increased antioxidant enzyme activity in stored broccoli. Compared to the control group (CK), transcriptomic and metabolic analyses of the composite treatment group (LED‐BVOCs) on the 4 days of storage identified 3/1, 7/0, 1/1, 20/6, and 6/1 differentially expressed genes (DEGs)/differentially abundant metabolites in metabolic pathways related to ascorbic acid, glutathione, glucosinolate, polyphenols, and methionine, respectively, and there were 4 DEGs involved in the regulation of senescence during ethylene signal transduction. It is evident that LED‐BVOCs treatment can effectively regulate the metabolic processes of antioxidants in broccoli during storage. Overall, the LED‐BVOCs treatment improved the antioxidant capacity and reduced ethylene production in stored broccoli, thus delaying senescence. Our study provides new insights on the ability of using BVOCs as biopreservatives to improve the antioxidant capacity and delay senescence of broccoli during storage.
Plasma signature metabolites of dietary fat intake characterize associations with prevalent metabolic syndrome
Authors: Wan Xuzhi , Shi Hongbo , Jia Wei , Zhu Li , Tian Yimei , Meng Denghui , Wang Anli , Li Yaoran , Liu Xiaohui , Li Haoyu , Zhang Lange , Zhuang Pan , Zhang Yu , Jiao Jingjing 
Published: October 2024

AbstractThe metabolic response to dietary fat intake and its relation with metabolic syndrome (MetS) remains unclear. Here, we identified dietary fat related signature metabolites and characterized their associations with MetS prevalence. We enrolled 236 participants from the Precision Nutrition and Food Safety for Dietary Prevention of Chronic Disease study in China. Nontargeted metabolomics was conducted to investigate plasma metabolome. The signature metabolites in relation to dietary fat intake were assessed using elastic net regression with a 10‐fold cross‐validation. We identified multi‐metabolite profiles comprising of 28, 19, 23, 31, and 25 metabolites, which were robustly correlated with dietary intake of saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids (PUFAs), n − 3 PUFAs, and n − 6 PUFAs, respectively (r = .47–.54; p < .001). After adjustment for potential risk factors, metabolic signatures of PUFAs and n − 3 PUFAs were inversely associated with 31% and 48% lower MetS prevalence, respectively. Dietary intake of n − 3 and n − 6 PUFAs ameliorates key metabolites involved in the glyoxylate and dicarboxylate metabolism, arginine biosynthesis, and tricarboxylic acid cycle. Our findings revealed plasma metabolic signatures characterizing dietary fat intake and supported the beneficial role of PUFAs especially n − 3 PUFAs in MetS prevention.
The Prognostication of Surgically Resected Head and Neck Squamous Cell Carcinoma Using Pre‐Therapy Neutrophil and Lymphocyte Counts
Authors: Sunkara Pranit R. , Chow Emily , Waitzman Jacob , Sukari Ammar , Cramer John D. 
Published: October 2024

ABSTRACTBackgroundHematological markers, such as neutrophils (ANC), lymphocyte (ALC), and neutrophil‐lymphocyte ratio (NLR), may serve as indicators of systemic inflammation and immune response in head and neck squamous cell carcinoma (HNSCC). However, their prognostic significance across HNSCC subtypes remains to be fully elucidated.MethodsWe conducted a secondary analysis of a randomized clinical trial involving patients with surgically resected HNSCC with either positive margins or extranodal extension. These patients received either adjuvant chemoradiation with or without lapatinib. We explored the correlation between pre‐therapy ANC, ALC, and NLR levels and overall survival (OS) as well as disease‐free survival (DFS). A sub‐group analysis examined potential links between these markers, primary tumor location, and HPV status.ResultsOf the 688 patients in the trial, we included 681 patients with documented pre‐therapy ANC and ALC values. High pre‐therapy ANC and ALC were significantly associated with reduced OS (HR, 1.56; 95% CI: 1.19–2.05) and (HR, 1.34; 95% CI: 1.01–1.79), respectively. High NLR did not significantly affect OS (HR, 1.09; 95% CI: 0.81–1.47). Subgroup analysis indicated significantly reduced OS in patients with high ANC across oropharyngeal, non‐oropharyngeal, and HPV‐negative subtypes. High ANC, ALC, and NLR did not impact DFS notably.ConclusionElevated pre‐therapy ANC is strongly associated with decreased survival across all patients and subgroups, ALC was only significant in the general patient analysis. NLR's association with reduced OS was not statistically significant. These biomarkers may provide greater prognostic value in patients with oropharyngeal cancer and seemed to be more strongly associated with OS than DFS.Trial RegistrationClinicaltrials.gov identifier: NCT00424255; URL: https://clinicaltrials.gov/ct2/show/study/NCT00424255
Ninjurin1 deficiency differentially mitigates colorectal cancer induced by azoxymethane and dextran sulfate sodium in male and female mice
Authors: Song Chin‐Hee , Kim Nayoung , Nam Ryoung Hee , Choi Soo In , Jang Jae Young , Kim Eun Hye , Ha Sungchan , Shin Eun , Choi Hoon , Kim Kyu‐Won , Jeon Sejin , Oh Goo Taeg , Seok Yeong‐Jae 
Published: October 2024

AbstractThis study investigated the role of Ninjurin1 (Ninj1), encoding a small transmembrane protein, in colitis‐associated colon tumorigenesis in relation to sex hormones. Male and female wild‐type (WT) and Ninj1 knockout (KO) mice were treated with azoxymethane (AOM) and dextran sulfate sodium (DSS), with or without testosterone propionate (TP). At week 2 (acute colitis stage), Ninj1 KO exhibited an alleviation in the colitis symptoms in both male and female mice. The M2 macrophage population increased and CD8+ T cell population decreased only in the female Ninj1 KO than in the female WT AOM/DSS group. In the female AOM/DSS group, TP treatment exacerbated colon shortening in the Ninj1 KO than in the WT. At week 13 (tumorigenesis stage), male Ninj1 KO mice had fewer tumors, but females showed similar tumors. In the WT AOM/DSS group, females had more M2 macrophages and fewer M1 macrophages than males, but this difference was absent in Ninj1 KO mice. In the Ninj1 KO versus WT group, the expression of pro‐inflammatory mediators and Ho‐1 and CD8+ T cell populations decreased in both female and male Ninj1 KO mice. In the WT group, M2 macrophage populations were increased by AOM/DSS treatment and decreased by TP treatment. However, neither treatment changed the cell populations in the Ninj1 KO group. These results suggest that Ninj1 is involved in colorectal cancer development in a testosterone‐dependent manner, which was different in male and female. This highlights the importance of considering sex disparities in understanding Ninj1's role in cancer pathogenesis.
A Study of Data Processing Methods for Non‐Contact Multispectral Method of Blood Oxygen Saturation
Authors: Yizhuo Zhao , Yu Ren , Hongxing Cai , Tingting Wang , Dongliang Li , Yu Wang , Jianguo Liu , Teng Li , Yangyang Hua 
Published: October 2024

ABSTRACTRegular monitoring of blood oxygenation is important for disease prevention and treatment. Image photoplethysmography (IPPG) technology is a non‐contact physiological parameter detection technology, which has been widely used in blood oxygenation detection. However, traditional imaging devices still have issues such as low detection accuracy, narrower receiving spectral range. In this paper, we proposed two improved detection methods based on the dual‐wavelength measurement principle, that is, dual‐band IPPG signal ratio method and dual‐band IPPG signal AC/DC method. To verify the effectiveness of the two methods, we used different heartbeat period IPPG signals as sample data sets, and combined PLS and RF algorithms for model training, thus obtaining the best data processing method. The experimental results showed that the dual‐band IPPG signal AC/DC method can effectively reduce the model training time. This method meets the strong demand for non‐contact blood oxygen measurement and provides a new measurement idea.
The Role of Propofol in Alcohol Withdrawal Syndrome: A Systematic Review
Authors: Shirk Logan , Reinert Justin P. 
Published: October 2024

AbstractThe objective of this review was to evaluate the efficacy and safety of propofol in the treatment of critically ill patients diagnosed with alcohol withdrawal syndrome (AWS). A review was conducted in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses) criteria, and Embase, MEDLINE (PubMed), Cochrane CENTRAL, and Web of Science were queried for results through June 2024. Studies providing efficacy or safety data associated with propofol with a reported diagnosis of AWS in critically ill patients were included. Studies evaluating pediatric patients, those without quantitative and qualitative outcome data, and those not readily translatable to English were excluded. Five retrospective cohort analyses of 218 patients were included in this systematic review. Patients were found to have both significant and non‐significant increases in time to resolution of AWS symptoms when treated with propofol versus the AWS standard of care. Adjunct treatment with propofol was generally associated with reductions in total benzodiazepine use and increases in both ICU length of stay and duration of mechanical ventilation. The results of this systematic review provide the evidence necessary to support the use of propofol as an efficacious and safe medication in the management of severe and refractory AWS. Further investigation is required to determine optimal dosing strategies and durations of therapy. The results of this systematic review demonstrate the clinical utility of propofol as part of the management strategy for severe and refractory AWS.
Pharmacokinetics and Safety of Intravenous Candidate Biosimilar CT‐P47 and Reference Tocilizumab: A Randomized, Double‐Blind, Phase 1 Study
Authors: Haranaka Miwa , Eto Takashi , Tanaka Takanori , Yazawa Rie , Burmester Gerd , Keystone Edward , Kim SungHyun , Bae YunJu , Suh JeeHye , Yang GoEun , Kim YunAh , Lee JaeYong , Smolen Josef S. 
Published: October 2024

AbstractCT‐P47 is a candidate biosimilar of tocilizumab. This 12‐week, randomized, double‐blind, parallel‐design, phase 1 study aimed to demonstrate pharmacokinetic (PK) equivalence of CT‐P47 and reference tocilizumab. Participants were healthy Japanese adults aged 18‐55 years. Participants were randomized (1:1:1) to receive a single intravenous dose (8 mg/kg) of CT‐P47, EU‐approved tocilizumab (EU‐tocilizumab), or US‐licensed tocilizumab (US‐tocilizumab). Primary PK endpoints were area under the concentration–time curve (AUC) from time zero to infinity, AUC from time zero to the last quantifiable concentration, and maximum serum concentration. Additional PK variables, safety, and immunogenicity were evaluated. The study was conducted from January 20 to May 26, 2023, in three centers in Japan. In total, 133 male participants were randomized (n = 45 to CT‐P47, n = 44 to EU‐tocilizumab, and n = 44 to US‐tocilizumab). For all primary PK variables, 90% confidence intervals of the ratio of geometric least squares means were within the predefined equivalence margin of 0.80‐1.25. Secondary PK variables were similar across groups. The most common treatment‐emergent adverse event (TEAE) was neutrophil count decreased, occurring in 15 (33.3%), 13 (30.2%), and 12 (27.3%) participants in the CT‐P47, EU‐tocilizumab, and US‐tocilizumab groups, respectively. There were no serious TEAEs, deaths, or study drug discontinuations due to TEAEs. Few participants were anti‐drug antibody (ADA)‐ or neutralizing antibody (NAb)‐positive. At the end of study, four (8.9%), one (2.3%), and two (4.5%) participants in the CT‐P47, EU‐tocilizumab, and US‐tocilizumab groups, respectively, were ADA‐positive; two (4.4%), zero (0%), and one (2.3%) in the respective groups were NAb‐positive. CT‐P47 demonstrated PK equivalence and comparable safety to EU‐ and US‐tocilizumab.
Correlation Between Anxiety, Depression and Hemodynamic Changes in Office‐Based Laryngeal Surgery
Authors: Hamdan Abdul‐Latif , Ghzayel Lana , Abou Raji Feghali Patrick , Abi Zeid Daou Christophe , Semaan Zeina Maria , Mourad Marc 
Published: October 2024

ObjectiveTo investigate the correlation between anxiety, depression, and hemodynamic changes during office‐based laryngeal surgery (OBLS).MethodsAll patients undergoing OBLS between February 2024 until July 2024 were invited to participate in the study. Participants had their vital signs recorded throughout the procedure at a 5‐min interval. They also had to fill the Generalized Anxiety Disorder scale‐7 (GAD‐7) to assess anxiety severity and the Patient Health Questionnaire‐9 (PHQ‐9) to assess depression severity. Demographic data included age, gender, history of smoking, history of reflux disease, history of cardiovascular diseases, type and duration of procedure.ResultsA total of 45 patients were recruited in the study. During OBLS, 35.5% of patients developed hypertension and 28.9% developed tachycardia. There was a significant increase in mean systolic blood pressure (SBP) by 30.16 mmHg (p < 0.001), in mean diastolic blood pressure (DBP) by 31.44 mmHg (p < 0.001), and in mean heart rate (HR) by 14.2 beats per minute (p < 0.001). There was also a significant decrease in the mean O2 saturation by 0.4% (p = 0.001). There was no correlation between anxiety and SBP, DBP, HR, and O2 (r < 0.1). There was also no correlation between depression levels and SBP, DBP, HR, O2 (r < 0.1).ConclusionThere was a significant increase in the mean SBP, DP, and HR and a significant decrease in the O2 saturation in patients undergoing OBLS. However, there was no correlation between anxiety, depression and the changes in these vital signs. Future investigations are needed to understand the causes of hemodynamic instability in OBLS.Level of Evidence2 Laryngoscope, 135:818–822, 2025
Deciphering the Conformations of Glutathione Oxidized Peptide: A Comparative NMR Study in Solution and Solid‐State Environments
Authors: Senapati Dillip K. , Yarava Jayasubba Reddy , Ramanathan K. V. , Raghothama S. 
Published: October 2024

ABSTRACTGlutathione (GSH) and its oxidized dimer (GSSG) play an important role in living systems as an antioxidant, balancing the presence of reactive oxygen species (ROS). The central thiol (‐S‐S‐) bond in GSSG can undergo free rotation, providing multiple conformations with respect to the S‐S bridge. The six titratable sites of GSSG, which are influenced by pH variations, affect these conformations in solution, whereas in solids, additionally crystal packing effects come into play. In view of differing reports about the structure of GSSG in literature, we have here conducted an extensive reexamination of its conformations using NMR, and contrasting results have been obtained for solution and solid state. In solution, the existence of more than one antiparallel orientation of the monomer unit with different hydrogen bonding schemes has been indicated by NOE and amide temperature coefficient results. On the other hand, in the solid‐state, a 1H‐1H double‐quantum (DQ) to 13C single‐quantum (SQ) correlation study has confirmed a parallel orientation, consistent with the reported X‐ray crystal structure. Experimentally assigned solid‐state NMR resonances have been validated using GIPAW calculations incorporated in the Quantum ESPRESSO package.
Understanding the Interactions of Nanoparticles and Dissolved Organic Matter at the Molecular Level by 1H 2D Multi‐Exponential Transverse Relaxation NMR Spectroscopy
Authors: Dia Malak , Farjon Jonathan , Raveleau Clotilde , Simpson André , Peyneau Pierre‐Emmanuel , Béchet Béatrice , Courtier‐Murias Denis 
Published: October 2024

ABSTRACTThe interaction between humic acid (HA) and engineered nanoparticles (NPs) is critical in environmental sciences, especially for understanding the behavior of NPs in natural waters. This study employs 1H 2D Multi‐Exponential Transverse Relaxation (METR) NMR spectroscopy to examine the molecular‐level interactions between Pahokee Peat humic acid (HA) and carboxyl‐functionalized iron oxide nanoparticles (NPCOs). First, 1H 2D METR NMR spectroscopy allowed not only the identification of HA in terms of its chemical composition but also the separation of molecules with the same chemical shift values but different rates of molecular tumbling. Then, using solutions with varying NPCO concentrations (0, 10, 40, and 100 μM), we observed significant changes in the T2 relaxation times of HA components, indicating interactions between HA and NPCO. Analysis showed the biggest effect on two chemical shift regions, corresponding to lipids and carbohydrates, revealing that smaller molecules within these regions exhibit the most significant changes in T2 values upon the addition of NPCO. This suggests that these molecules are the initial sites of interaction, with the entire HA system being affected at higher NPCO concentrations. These findings highlight the utility of METR NMR spectroscopy in studying complex environmental mixtures and provide insights into the behavior of HA and NPs, essential for understanding the fate of NPs in the environment.
First Principles Study of Electronic and Optical Properties of Al‐P Co‐Doped ZnO in the Presence of Zn Vacancies
Authors: Guo Zhengguang , Yao Yonghong , Liu Jin 
Published: October 2024

AbstractThe BP neural network optimized by the Adam algorithm was used to predict the defect formation energy of Al−P co‐doped ZnO systems with different concentrations of P replacing O under the presence of different concentrations of VZn. It was found that the easily formed AlZnPo‐1VZn, AlZnPO‐2VZn, and AlZn2PO‐1VZn systems. The first principles of density function were used to study the geometric, electronic, and optical properties of each system. The simulation results show that the bandgap values of the three systems have decreased relative to the intrinsic ZnO, among which AlZnPO‐1VZn and AlZnPO‐2VZn is still a p‐type conductive system, AlZnPO‐2VZn has the highest conductivity. From the analysis of reflectivity, absorption rate, and light transmittance, AlZn2PO‐1VZn has the most relatively excellent optical properties, followed by AlznPo‐2VZn.
Investigating Predictors of Decannulation Through Endoscopic Approach in Patients With Tracheostomy and Peristomal Subglottic Stenosis
Authors: Wehbi Nader , Ahmadian David , Gleadhill Claire , Yip Helena T. 
Published: October 2024

AbstractObjectivePeristomal subglottic stenosis (SGS) is a common sequela after tracheostomy, with severe cases precluding decannulation. Predictors of decannulation success in these patients following endoscopic intervention are not well studied. The aim of this study is to investigate predictors of successful decannulation and inform treatment decisions.Study DesignThis study is a retrospective case series of 22 adult patients presenting to the senior author with a tracheostomy and peristomal SGS precluding decannulation between 2018 and 2023.SettingDepartment of Otolaryngology–Head & Neck Surgery, University of Arizona College of Medicine–Tucson.MethodsPatient demographics, relevant clinical factors, stenosis characteristics, and the number of endoscopic procedures performed were analyzed to identify predictors of successful decannulation. Endoscopic interventions were generally performed 3 months apart with CO2 laser debridement, balloon dilation, and intralesional injection of steroid, all done with a laser‐safe endotracheal tube in place through the stoma.ResultsOut of the 22 patients in the study, 9 (40.9%) achieved decannulation, all through an endoscopic approach. Body mass index (BMI) and age were identified as significant negative predictors of decannulation success (P = .02; P = .05, respectively). Stenosis characteristics, such as the presence of tracheomalacia, excessive dynamic airway collapse, multilevel stenosis, posterior glottic stenosis, and anterior granulation tissue shelf did not significantly impact decannulation success.ConclusionA 40.9% decannulation rate was achieved in our cohort. BMI and age were identified as negative predictors of decannulation success. Stenosis characteristics did not significantly affect decannulation outcomes. Further investigation is warranted to establish reliable predictors of decannulation.
Pediatric patients with von Hippel–Lindau and hemangioblastomas treated successfully with belzutifan
Authors: Duan Emily , Robinson Michael , Davis Charles , Pruthi Sumit , Shin Christina , Lewis Marisa , Martinez‐Agosto Julian A. , Gorin Michael B. , Shuch Brian M. , Friedman Debra L. , Chang Vivian Y. 
Published: October 2024

AbstractHemangioblastoma is the most common tumor associated with von Hippel–Lindau (VHL), and are a leading cause of mortality. We present five pediatric patients with VHL‐associated hemangioblastomas treated with belzutifan, a hypoxia‐inducible factor 2a (HIF2a) inhibitor. Three patients were started on belzutifan due to vision loss from progressive retinal hemangioblastomas. Within one year of treatment, all three patients had improvement in hemangioblastoma size and visual acuity. For patients with intracranial lesions, belzutifan resulted in an improvement in neurologic symptoms and hemangioblastoma size. Four patients experienced grade 1–2 anemia and two patients required a dose reduction. Our report suggests that belzutifan can be an effective therapy for pediatric, adolescent, and young adult patients with VHL‐associated hemangioblastomas.