bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
Authors: Chabert Adrien , Damien Pauline , Verhoeven Paul O. , Grattard Florence , Berthelot Philippe , Zeni Fabrice , Panicot-Dubois Laurence , Robert Stéphane , Dignat-George Françoise , Eyraud Marie-Ange , Pozzetto Bruno , Payrastre Bernard , Cognasse Fabrice , Garraud Olivier , Hamzeh-Cognasse Hind 
Published: July 2017

AbstractBeyond their hemostatic functions, platelets alter their inflammatory response according to the bacterial stimulus.Staphylococcus aureusis associated with exacerbated inflammation and thrombocytopenia, which is associated with poor prognosis during sepsis. Acetylsalicylic acid and statins prevent platelet aggregation and decrease the mortality rate during sepsis. Therefore, we assessed whether these two molecules could reducein vitroplatelet activation and the inflammatory response toS. aureus. Platelets were exposed to clinical strains ofS. aureusin the presence or absence of acetylsalicylic acid or fluvastatin. Platelet activation, aggregation, and release of soluble sCD62P, sCD40 Ligand, RANTES and GROα were assessed. Platelet cell death was evaluated by analyzing the mitochondrial membrane potential, phosphatidylserine exposure, platelet microparticle release and caspase-3 activation. AllS. aureusstrains induced platelet activation but not aggregation and decreased the platelet count, the expression of cell death markers and the release of RANTES and GROα. Acetylsalicylic acid but not fluvastatin limited platelet activation and inflammatory factor release and restored the platelet count by protecting platelets fromStaphylococcus-induced expression of cell death markers. This study demonstrates that acetylsalicylic acid limitsS. aureus-induced effects on platelets by reducing cell death, revealing new strategies to reduce the platelet contribution to bacteremia-associated inflammation.
Ferroelectric-Paraelectric Transition In A Membrane With Quenched-Induced δ-Phase Of PVDF
Authors: García-Zaldívar O. , Escamilla-Díaz T. , Ramírez-Cardona M. , Hernández-Landaverde M. A. , Ramírez-Bon R. , Yañez-Limón J. M. , Calderón-Piñar F. 
Published: July 2017

AbstractThe stabilization of δ-phase of poly(vinylidene fluoride) PVDF in a 14 µm-thickness ferroelectric membrane is achieved by a simple route based on the use of a dimethylformamide (DMF)/acetone solvent, in which the application of external electric field is not required. X-ray diffraction and calorimetric experiments on heating reveal that, at 154 °C, the original mixture between ferroelectric δ-phase and paraelectric α-phase transits to a system with only this latter phase in the crystalline fraction. A gradual and slight increment of amorphous fraction up to the melting at 161 °C is also observed. The existence of δ-phase is corroborated by the occurrence of a broad maximum around 154 °C in dielectric permittivity measurements, as well as the hysteresis loops observed at room temperature. These results suggest a wide thermal window for a stable δ-phase, between room temperature and 154 °C, a subsequent transition into α-phase and the corresponding melting at 161 °C. The broad dielectric maximum observed around 154 °C in dielectric and calorimetric measurements, can be associated with a diffuse ferroelectric-paraelectric transition.
FTY720 Induces Autophagy-Associated Apoptosis in Human Oral Squamous Carcinoma Cells, in Part, through a Reactive Oxygen Species/Mcl-1-Dependent Mechanism
Authors: Bai Li-Yuan , Chiu Chang-Fang , Chiu Shih-Jiuan , Chu Po-Chen , Weng Jing-Ru 
Published: July 2017

AbstractIn this study, we interrogated the mechanism by which the immunosuppressant FTY720 mediates anticancer effects in oral squamous cell carcinoma (OSCC) cells. FTY720 differentially suppressed the viability of the OSCC cell lines SCC4, SCC25, and SCC2095 with IC50 values of 6.1, 6.3, and 4.5 μM, respectively. This antiproliferative effect was attributable to the ability of FTY720 to induce caspase-dependent apoptosis. Mechanistic evidence suggests that FTY720-induced apoptosis was associated with its ability to inhibit Akt-NF-κB signaling, to facilitate the proteasomal degradation of the antiapoptotic protein Mcl-1, and to increase reactive oxygen species (ROS) generation. Both overexpression of Mcl-1 and inhibition of ROS partially protected cells from FTY720-induced caspase-9 activation, PARP cleavage and cytotoxicity. In addition, FTY720 induced autophagy in OSCC cells, as manifested by LC3B-II conversion, decreased p62 expression, and accumulation of autophagosomes. Inhibition of autophagy by bafilomycin A1 protected cells from FTY720-induced apoptosis. Together, these findings suggest an intricate interplay between autophagy and apoptosis in mediating the tumor-suppressive effect in OSCC cells, which underlies the translational potential of FTY720 in fostering new therapeutic strategies for OSCC.
β-caryophyllene emitted from a transgenic Arabidopsis or chemical dispenser repels Diaphorina citri, vector of Candidatus Liberibacters
Authors: Alquézar Berta , Volpe Haroldo Xavier Linhares , Magnani Rodrigo Facchini , de Miranda Marcelo Pedreira , Santos Mateus Almeida , Wulff Nelson Arno , Bento Jose Mauricio Simões , Parra José Roberto Postali , Bouwmeester Harro , Peña Leandro 
Published: July 2017

AbstractProduction of citrus, the main fruit tree crop worldwide, is severely threatened by Huanglongbing (HLB), for which as yet a cure is not available. Spread of this bacterial disease in America and Asia is intimately connected with dispersal and feeding of the insect vector Diaphorina citri, oligophagous on rutaceous host plants. Effective control of this psyllid is an important component in successful HLB management programs. Volatiles released from the non-host guava have been shown to be repellent to the psyllid and to inhibit its response to citrus odour. By analysing VOC emission from guava we identified one volatile compound, (E)-β-caryophyllene, which at certain doses exerts a repellent effect on D. citri. Non-host plant rejection mediated by (E)-β-caryophyllene is demonstrated here by using Arabidopsis over-expression and knock-out lines. For the first time, results indicate that genetically engineered Arabidopsis plants with modified emission of VOCs can alter the behaviour of D. citri. This study shows that transgenic plants with an inherent ability to release (E)-β-caryophyllene can potentially be used in new protection strategies of citrus trees against HLB.
The aspirin metabolite salicylate inhibits lysine acetyltransferases and MUC1 induced epithelial to mesenchymal transition
Authors: Fernandez Harvey R. , Lindén Sara K. 
Published: July 2017

AbstractMUC1 is a transmembrane mucin that can promote cancer progression, and its upregulation correlates with a worse prognosis in colon cancer. We examined the effects of overexpression of MUC1 in colon cancer cells, finding that it induced epithelial to mesenchymal transition (EMT), including enhanced migration and invasion, and increased Akt phosphorylation. When the clones were treated with the aspirin metabolite salicylate, Akt phosphorylation was decreased and EMT inhibited. As the salicylate motif is necessary for the activity of the lysine acetyltransferase (KAT) inhibitor anacardic acid, we hypothesized these effects were associated with the inhibition of KAT activity. This was supported by anacardic acid treatment producing the same effect on EMT. In vitro KAT assays confirmed that salicylate directly inhibited PCAF/Kat2b, Tip60/Kat5 and hMOF/Kat8, and this inhibition was likely involved in the reversal of EMT in the metastatic prostate cancer cell line PC-3. Salicylate treatment also inhibited EMT induced by cytokines, illustrating the general effect it had on this process. The inhibition of both EMT and KATs by salicylate presents a little explored activity that could explain some of the anti-cancer effects of aspirin.
A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
Authors: Trevisan-Silva Dilza , Bednaski Aline V. , Fischer Juliana S.G. , Veiga Silvio S. , Bandeira Nuno , Guthals Adrian , Marchini Fabricio K. , Leprevost Felipe V. , Barbosa Valmir C. , Senff-Ribeiro Andrea , Carvalho Paulo C. 
Published: July 2017

AbstractVenoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523.
Evaluation des spanischen Diagnostik-Tools für die Komplexität in der Palliativmedizin, IDC-Pal, durch Hausärzte in Deutschland: Eine Pilotstudie
Authors: Comino María , Garcia Victor , López Maria , Feddersen Berend , Roselló María , Sanftenberg Linda , Schelling Jörg 
Published: July 2017

Zusammenfassung
Hintergrund Die Palliativmedizin ist ein essentieller Bestandteil der medizinischen Versorgung einer Gesellschaft. Unter idealen Bedingungen erfolgt diese von der Primärversorgung (Hausärzte und Pflegedienste) mit palliativmedizinischer Basisqualifikation. Hierbei ist es oft schwierig zu identifizieren, welche Patienten von einem spezialisierten Palliativ-Care Team profitieren würden. Zur Beurteilung der Komplexitätssituation eines palliativen Patienten stellen wir das spanische Diagnostik-Tool IDC-Pal (Instrumento Diagnóstico de la Complejidad en Cuidados Paliativos) vor, welches versucht zu spezifizieren wann, warum und wohin ein/-e palliative/-r Patient/-in überwiesen werden sollte. Die Ziele dieser Studie waren daher zum einen die deutsche Übersetzung und kulturelle Anpassung des bisher in Spanien verwandten Diagnostik-Tools IDC-Pal, sowie die Überprüfung der Machbarkeit und der Augenschein Validität dieses Diagnosetools.
Methoden In der ersten Phase der Studie erfolgten die Vorwärts-Rückwärts-Übersetzung mit sprachlicher und kultureller Anpassung von IDC-Pal, sowie die Validierung des Inhalts durch ein Revisionskomitee. In der zweiten Phase wurde die vorläufige Version des Diagnostiktools von 38 Hausärzten mithilfe einer 10-stufigen Likert-Skala (1= „auf keinen Fall einverstanden“ und 10= „völlig einverstanden“) qualitativ bewertet. Entsprechend wurde abschließend eine endgültige Version des Diagnostiktools IDC-Pal entwickelt.
Ergebnisse Die Übersetzung und die Anpassung konnten ohne Probleme verwirklicht werden. Die Machbarkeit und die Augenschein Validität des Diagnostiktools IDC-Pal wurden als überdurchschnittlich gut eingestuft. So betrug der Mittelwert der globalen Antworten in der Likert-Skala 7,79 mit einer Standardabweichung (SD) von 0,36. Die Teilnehmer bestätigten ebenfalls die Augenschein Validität mit einem Durchschnitt von 7,82 und einer SD von 0,26 und eine Machbarkeit des Werkzeugs mit einem Durchschnitt von 7,79 und einer SD von 0,39.
Schlussfolgerungen Eine konzeptionelle, kulturelle und sprachlich gleichwertige Version des Diagnostiktools IDC-Pal konnte entwickelt werden. Die befragten deutschen Hausärzte waren sich einig, dass sie mit dem IDC-Pal Tool die Komplexitätssituation von palliativen Patienten einschätzen können. Diese Ergebnisse zeigen, dass die Ärzte in Bayern und eventuell in Deutschland von der vollständigen Validierung von IDC-Pal profitieren könnten.
Correction of Pelvic Tilt and Pelvic Rotation in Cup Measurement after THA – An Experimental Study
Authors: Schwarz Timo Julian , Weber Markus , Dornia Christian , Worlicek Michael , Renkawitz Tobias , Grifka Joachim , Craiovan Benjamin 
Published: July 2017

Purpose Accurate assessment of cup orientation on postoperative pelvic radiographs is essential for evaluating outcome after THA. Here, we present a novel method for correcting measurement inaccuracies due to pelvic tilt and rotation.
Method In an experimental setting, a cup was implanted into a dummy pelvis, and its final position was verified via CT. To show the effect of pelvic tilt and rotation on cup position, the dummy was fixed to a rack to achieve a tilt between + 15° anterior and -15° posterior and 0° to 20° rotation to the contralateral side. According to Murray’s definitions of anteversion and inclination, we created a novel corrective procedure to measure cup position in the pelvic reference frame (anterior pelvic plane) to compensate measurement errors due to pelvic tilt and rotation.
Results The cup anteversion measured on CT was 23.3°; on AP pelvic radiographs, however, variations in pelvic tilt (± 15°) resulted in anteversion angles between 11.0° and 36.2° (mean error 8.3°± 3.9°). The cup inclination was 34.1° on CT and ranged between 31.0° and 38.7° (m. e. 2.3°± 1.5°) on radiographs. Pelvic rotation between 0° and 20° showed high variation in radiographic anteversion (21.2°–31.2°, m. e. 6.0°± 3.1°) and inclination (34.1°–27.2°, m. e. 3.4°± 2.5°). Our novel correction algorithm for pelvic tilt reduced the mean error in anteversion measurements to 0.6°± 0.2° and in inclination measurements to 0.7° (SD± 0.2). Similarly, the mean error due to pelvic rotation was reduced to 0.4°± 0.4° for anteversion and to 1.3°± 0.8 for inclination.
Conclusion Pelvic tilt and pelvic rotation may lead to misinterpretation of cup position on anteroposterior pelvic radiographs. Mathematical correction concepts have the potential to significantly reduce these errors, and could be implemented in future radiological software tools.
Key Points
Citation Format
Modern Cartilage Imaging of the Ankle
Authors: Weber Marc-André , Wünnemann Felix , Jungmann Pia , Kuni Benita , Rehnitz Christoph 
Published: July 2017

Background Talar osteochondral lesions are an important risk factor for the development of talar osteoarthritis. Furthermore, osteochondral lesions might explain persistent ankle pain. Early diagnosis of accompanying chondral defects is important to establish the optimal therapy strategy and thereby delaying or preventing the onset of osteoarthritis. The purpose of this review is to explain modern cartilage imaging with emphasis of MR imaging as well as the discussion of more sophisticated imaging studies like CT-arthrography or functional MR imaging.
Methods Pubmed literature search concerning: osteochondral lesions, cartilage damage, ankle joint, talus, 2 D MR imaging, 3 D MR imaging, cartilage MR imaging, CT-arthrography, cartilage repair, microfracture, OATS, MACT.
Results and Conclusion Dedicated MR imaging protocols to delineate talar cartilage and the appearance of acute and chronic osteochondral lesions were discussed. Recent developments of MR imaging, such as isotropic 3 D imaging that has a higher signal-to noise ratio when compared to 2 D imaging, and specialized imaging methods such as CT-arthrography as well as functional MR imaging were introduced. Several classifications schemes and imaging findings of osteochondral lesions that influence the conservative or surgical therapy strategy were discussed. MRI enables after surgery the non-invasive assessment of the repair tissue and the success of implantation.
Key points
Citation Format
Biological annihilation via the ongoing sixth mass extinction signaled by vertebrate population losses and declines
Authors: Ceballos Gerardo , Ehrlich Paul R. , Dirzo Rodolfo 
Published: July 2017

SignificanceThe strong focus on species extinctions, a critical aspect of the contemporary pulse of biological extinction, leads to a common misimpression that Earth’s biota is not immediately threatened, just slowly entering an episode of major biodiversity loss. This view overlooks the current trends of population declines and extinctions. Using a sample of 27,600 terrestrial vertebrate species, and a more detailed analysis of 177 mammal species, we show the extremely high degree of population decay in vertebrates, even in common “species of low concern.” Dwindling population sizes and range shrinkages amount to a massive anthropogenic erosion of biodiversity and of the ecosystem services essential to civilization. This “biological annihilation” underlines the seriousness for humanity of Earth’s ongoing sixth mass extinction event.
WIsH: who is the host? Predicting prokaryotic hosts from metagenomic phage contigs
Authors: Galiez Clovis , Siebert Matthias , Enault François , Vincent Jonathan , Söding Johannes 
Published: July 2017

Abstract

Summary
WIsH predicts prokaryotic hosts of phages from their genomic sequences. It achieves 63% mean accuracy when predicting the host genus among 20 genera for 3 kbp-long phage contigs. Over the best current tool, WisH shows much improved accuracy on phage sequences of a few kbp length and runs hundreds of times faster, making it suited for metagenomics studies.

Availability and implementation
OpenMP-parallelized GPL-licensed C ++ code available at https://github.com/soedinglab/wish.

Supplementary information
Supplementary data are available at Bioinformatics online.
ScanGEO: parallel mining of high-throughput gene expression data
Authors: Koeppen Katja , Stanton Bruce A , Hampton Thomas H 
Published: July 2017

Abstract

Summary
Current options to mine publicly available gene expression data deposited in NCBI’s gene expression omnibus (GEO), such as the GEO web portal and related applications, are optimized to reanalyze a single study, or search for a single gene, and therefore require manual intervention to reanalyze multiple studies for user-specified gene sets. ScanGEO is a simple, user-friendly Shiny web application designed to identify differentially expressed genes across all GEO studies matching user-specified criteria, for a flexible set of genes, visualize results and provide summary statistics and other reports using a single command.

Availability and implementation
The ScanGEO source code is written in R and implemented as a Shiny app that can be freely accessed at http://scangeo.dartmouth.edu/ScanGEO/. For users who would like to run a local instantiation of the app, the R source code is available under a GNU GPLv3 license at https://github.com/StantonLabDartmouth/AppScanGEO.

Supplementary information
Supplementary data are available at Bioinformatics online.
Are Older Adults Perceived as A Threat to Society? Exploring Perceived Age-Based Threats in 29 Nations
Authors: Ayalon Liat 
Published: July 2017

AbstractObjectivesThe present study adds to the current body of literature by simultaneously examining the public perception of young and old people as posing realistic threats (e.g., to the group’s power, resources, and welfare) and symbolic threats (e.g., to one’s world view, belief system and values).MethodsThe fourth wave of the European Social Survey was administered to individuals from 29 countries. Analysis is based on 56,170 individuals, who had data on the four relevant indicators. The study relied on a latent profile analysis to develop a typology of perceived realistic and symbolic threats to society by younger and older adults.ResultsA three-profile solution indicated that the perception of older and younger adults as threats to society often co-occurs. Sociodemographic characteristics at the individual-level and the Gini coefficient (e.g., an inequality indicator) at the country-level had differential associations with the profiles identified.ConclusionsThe study calls for a more balanced approach which evaluates attitudes toward both younger and older adults as potential sources of threat. Attention should be paid to individual- and national-level characteristics associated with age-based threats (e.g., the perception of a group, defined by its chronological age, as threatening).
A Report of 2 Cases of Brainstem Hemorrhage After Suboccipital Craniectomy for Chiari Decompression
Authors: Polster Sean P , Dougherty Mark C , Zeineddine Hussein A , Lee Seon-Kyu , Frim David 
Published: July 2017

Abstract

BACKGROUND AND IMPORTANCE
Decompression surgery for Chiari malformation is known to have very low procedure-related complications. There has been no report of post-Chiari malformation decompression surgery development of brainstem hemorrhage. We report 2 post-Chiari decompression surgery brainstem hemorrhage cases with 2-yr follow-up.

CLINICAL PRESENTATION
Two cases were reviewed in which patients underwent uncomplicated suboccipital craniectomy with expansive autologous pericranium duraplasty for Chiari decompression. Postoperatively, both patients awoke with hemibody sensory and motor deficits. Immediate postoperative magnetic resonance imaging revealed a small hemorrhage within the dorsal medulla in both cases. Follow-up imaging shows resolution along with near complete clinical recovery of deficits.

CONCLUSION
These cases demonstrate a rare postdecompression surgery-related complication in Chiari malformation. We hypothesize that these hemorrhages may occur from the rapid drainage of cerebrospinal fluid resulting in a loss of positive pressure, allowing a low-pressure hemorrhage to occur. Given that these hemorrhages are of low pressure, recovery is excellent.
Sex Differences in Cannabis Use Disorder Diagnosis Involved Hospitalizations in the United States
Authors: Zhu He , Wu Li-Tzy 
Published: July 2017

Objectives:
The study examined sex differences in trend and clinical characteristics of cannabis use disorder (CUD) diagnosis involved hospitalizations among adult patients.

Methods:
We analyzed hospitalization data from the 2007–2011 Nationwide Inpatient Samples for patients aged 18–64 years (N = 15,114,930). Descriptive statistics were used to characterize demographic variables and to compare the proportions of CUD diagnosis and comorbid patterns between male and female hospitalizations. Logistic regressions were performed to examine the association of sex and other demographic variables with CUD diagnosis.

Results:
During the study period, 3.3% of male and 1.5% of female hospitalizations had any-listed CUD diagnoses, and both sexes presented an upward trend in the number, rate, and proportion of CUD diagnosis. Among hospitalizations for patients aged 18–25 years, about 1 in 10 males and 1 in 20 females included a CUD diagnosis, and this proportion decreased with age strata. Mental disorders accounted for the highest proportion of CUD involved inpatient hospitalizations, and female CUD involved hospitalizations included a higher proportion of mental disorders that required hospitalized care compared with male hospitalizations (41% vs 36%). In each sex group, younger age, black race, lower household income, large metropolitan residence, non-private insurance, substance use diagnosis, and mental disorders were associated with elevated odds of having CUD diagnosis.

Conclusion:
The large sample of clinical hospitalization data suggest an increased trend in CUD diagnosis and sex differences in several comorbidities with CUD-involved hospital admissions. Prevention and treatment for CUD should consider sex differences in clinical comorbidities.