bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does resource availability affect host manipulation? – an experimental test with Schistocephalus solidus
Authors: HAFER NINA , BENESH DANIEL P. 
Published: January 2016

SUMMARYHost manipulation is a common strategy of parasites employed to increase their fitness by changing the phenotype of their hosts. Whether host manipulation might be affected by environmental factors such as resource availability, has received little attention. We experimentally infected laboratory-bred copepods with the cestodeSchistocephalus solidus, submitted infected and uninfected copepods to either a high or a low food treatment, and measured their behaviour. Infection reduced host activity and speed in both feeding treatments. However, the difference between the infected and uninfected copepods was smaller under low food conditions, because uninfected, but not infected, copepods moved slower under these conditions. We suggest that these differences are mediated by the physical condition of copepods rather than changes in how strongly the parasite manipulated host behaviour. Additionally, we measured three fitness-relevant traits (growth, development and infection rate in the next host) of the parasite to identify potential trade-offs with host manipulation. The largest parasites in copepods appeared the least manipulative, i.e. their hosts showed the smallest behavioural alterations, but this may again reflect variation in copepod condition, rather than life history trade-offs between parasite growth and host manipulation. Our results point to the possibility that parasite transmission depends on environmental conditions.
“Nor Mind Nor Body of Me Can Be Touched”: The Politics of Passivity in Moyshe Kulbak’sMontogand Samuel Beckett’sMurphy
Authors: Caplan Marc 
Published: January 2016

AbstractThis article will compare two novels: Moyshe Kulbak’sMontog(“Monday”) and Samuel Beckett’sMurphy. Each novel ends with the death of its protagonist, figured as both a senseless act and the apotheosis of its hero’s self-reflexive, ironic rejection of community, faith, and purpose. Drawing on theories of Hannah Arendt, this comparison proposes to read the two narratives and their preoccupation with incarceration, institutionalization, revolutionary activity, religion, and the family as profound yet oblique parables on the nature of privation, resistance, and commitment in the multiple senses. Indeed, by arguing on behalf of a “politics of failure,” this comparison proposes a methodology for reading Beckett and Kulbak postcolonially that in turn invites further consideration of the postcolonial status of expatriate Irish and early-Soviet Jewish cultures, respectively. This essay creates for the two narratives a community of elective affinity that neither author would have envisioned for himself, and thus demonstrates that their respective critiques of ideological progress—via their shared strategies of parody, linguistic marginality, and exile—fulfill an explicitly political function.
From butterboxes to wooden shoes: the shift in English popular sentiment from anti-Dutch to anti-French in the 1670s
Authors: Pincus Steven C. A. 
Published: January 2016

ABSTRACTWhile Restoration historians have traditionally assumed that there was little public interest in foreign affairs, and that English attitudes towards Europe were determined either by religious or domestic concerns, this essay argues that there was a lively and sophisticated English debate about Europe which turned on the proper identification of the universal monarch rather than religion. In the later 1660s the English political nation was deeply divided in its understanding of European politics. Enthusiastic supporters of the restored monarchy thought that the republican United Provinces sought universal dominion, while the monarchy's radical critics identified absolutist France as an aspirant to universal monarchy. French success in the early phases of the third Anglo-Dutch war, the failure of the French navy to support the English fleet at sea, and the overthrow of the Dutch republican regime in favour of William III, Prince of Orange, convinced the vast majority of the English that France represented the greater threat. Ultimately popular pressure compelled Charles II to abandon the French alliance. In addition, the popular conviction that Louis XIV had succeeded in corrupting the English court resulted in a new-found desire for popular accountability in foreign affairs, and a consequent diminution of the royal prerogative in that sphere.
SOCIO-ECONOMIC RIGHTS DURING ECONOMIC CRISES: A CHANGED APPROACH TO NON-RETROGRESSION
Authors: Warwick Ben TC 
Published: January 2016

AbstractWhen the UN Committee on Economic, Social and Cultural Rights (CESCR) released a letter in early 2012 addressing the financial and economic crises, it was long overdue. Finally, and around four and a half years after the crises began, the body responsible for monitoring those rights that had been most severely impacted had spoken. But what had been said? This article examines the alterations to the doctrine of non-retrogression that the 2012 Letter instigated. It does so by reference to the ‘Business as Usual’ and ‘accommodation’ theories of emergency response. The Letter to States is argued to have taken the Committee away from an approach to non-retrogression that treated times of normality and emergency in a similar way, and towards an approach that allows derogation-style deviations from the Covenant. This, it is argued, could have detrimental effects for the protection of economic and social rights. The difficulties in applying such an approach are considered.
On Active and Passive Testing
Authors: ALON NOGA , HOD RANI , WEINSTEIN AMIT 
Published: January 2016

Given a property of Boolean functions, what is the minimum number of queries required to determine with high probability if an input function satisfies this property or is ‘far’ from satisfying it? This is a fundamental question in property testing, where traditionally the testing algorithm is allowed to pick its queries among the entire set of inputs. Balcan, Blais, Blum and Yang have recently suggested restricting the tester to take its queries from a smaller random subset of polynomial size of the inputs. This model is called active testing, and in the extreme case when the size of the set we can query from is exactly the number of queries performed, it is known as passive testing.We prove that passive or active testing of k-linear functions (that is, sums of k variables among n over $\mathbb{Z}$2) requires Θ(k log n) queries, assuming k is not too large. This extends the case k = 1, (that is, dictator functions), analysed by Balcan, Blais, Blum and Yang.We also consider other classes of functions including low-degree polynomials, juntas, and partially symmetric functions. Our methods combine algebraic, combinatorial, and probabilistic techniques, including the Talagrand concentration inequality and the Erdős–Rado theorem on Δ-systems.
Out-of-Hospital Deliveries: A Five-Year Experience
Authors: Verdile Vincent P. , Tutsock Gregory , Paris Paul M. , Kennedy Robert A. 
Published: January 2016

AbstractIntroduction:Prehospital providers regularly encounter patients with obstetrical emergencies. This study determined the frequency and outcome of out-of-hospital deliveries in an urban, all advanced life support (ALS) emergency medical services (EMS) system.Methods:Retrospective review of all out-of-hospital records that involved women delivering babies in the care of prehospital providers from 1984–1988. The EMS system answered an average of 62,000 calls during the study period. The records of these patients were identified through a computer database.Results:A total of 81 out-of-hospital deliveries (1.4/month) occurred during the study years. The average age of the mothers was 24 years, and the average gestation period was 30 weeks. The women had an average of three previous pregnancies and two previous deliveries, and 10 were primagravida. Seventy-two (89%) of the deliveries occurred in the home. The paramedia encountered a variety of obstetrical and neonatal complications in 34% of the patient encounters. Nine neonates were delivered prior to the arrival of the paramedic team. Twenty-four neonates had Apgar scores calculated, and the one- and five-minute scores averaged eight and nine respectively. Five of the mothers had no prenatal care. Maternal complications included four patients noted to be hypertensive with the delivery, nine patients had some degree of vaginal bleeding, and in 33 patients, the prehospital providers did not deliver the placenta in the field. An EMS physician was in attendance for only two of the out-of-hospital deliveries.Discussion:In this urban EMS system, out-of-hospital deliveries, especially pre-term deliveries, are a common event. There appears to be a significant number of neonatal complications that confront paramedics. Generally, the paramedics were deficient in their documentation of the neonatal assessment. Continuing educational programs for paramedics should include reviewing normal and complicated vaginal deliveries as well as ALS measures for neonates. Protocols for obstetrical emergencies need to be developed and subjected to quality improvement measures.Conclusions:Paramedics, especially those in urban settings, are likely to encounter obstetrical and neonatal emergencies and a significant number of associated complications. Emergency medical services systems and medical directors should have in place continuing educational programs, patient-care protocols, and continuous quality improvement measures to evaluate the care rendered to patients having out-of-hospital deliveries.
The Link Between Glutamate and Migraine
Authors: Ramadan Nabih M. 
Published: January 2016

ABSTRACTMigraine pain-relay centers, including the trigeminal ganglion, trigeminal nucleus caudalis, and thalamus, contain glutamate-positive neurons, and glutamate activates the trigeminal nucleus caudalis. Glutamate is implicated in cortical spreading depression, trigeminovascular activation, and central sensitization. Glutamate receptor-subtype antagonists are effective in preclinical models of migraine, and in the clinic. These preclinical and clinical observations argue for a strong link between migraine and the glutamatergic system, a link that is important to further characterize in an effort to better understand migraine mechanisms and deliver effective therapies.
Bio-recognitive photonics of a DNA-guided organic semiconductor
Authors: Back Seung Hyuk , Park Jin Hyuk , Cui Chunzhi , Ahn Dong June 
Published: January 2016

AbstractIncorporation of duplex DNA with higher molecular weights has attracted attention for a new opportunity towards a better organic light-emitting diode (OLED) capability. However, biological recognition by OLED materials is yet to be addressed. In this study, specific oligomeric DNA–DNA recognition is successfully achieved by tri (8-hydroxyquinoline) aluminium (Alq3), an organic semiconductor. Alq3 rods crystallized with guidance from single-strand DNA molecules show, strikingly, a unique distribution of the DNA molecules with a shape of an ‘inverted’ hourglass. The crystal’s luminescent intensity is enhanced by 1.6-fold upon recognition of the perfect-matched target DNA sequence, but not in the case of a single-base mismatched one. The DNA–DNA recognition forming double-helix structure is identified to occur only in the rod’s outer periphery. This study opens up new opportunities of Alq3, one of the most widely used OLED materials, enabling biological recognition.
Quasi one-dimensional band dispersion and surface metallization in long-range ordered polymeric wires
Authors: Vasseur Guillaume , Fagot-Revurat Yannick , Sicot Muriel , Kierren Bertrand , Moreau Luc , Malterre Daniel , Cardenas Luis , Galeotti Gianluca , Lipton-Duffin Josh , Rosei Federico , Di Giovannantonio Marco , Contini Giorgio , Le Fèvre Patrick , Bertran François , Liang Liangbo , Meunier Vincent , Perepichka Dmitrii F. 
Published: January 2016

AbstractOn-surface covalent self-assembly of organic molecules is a very promising bottom–up approach for producing atomically controlled nanostructures. Due to their highly tuneable properties, these structures may be used as building blocks in electronic carbon-based molecular devices. Following this idea, here we report on the electronic structure of an ordered array of poly(para-phenylene) nanowires produced by surface-catalysed dehalogenative reaction. By scanning tunnelling spectroscopy we follow the quantization of unoccupied molecular states as a function of oligomer length, with Fermi level crossing observed for long chains. Angle-resolved photoelectron spectroscopy reveals a quasi-1D valence band as well as a direct gap of 1.15 eV, as the conduction band is partially filled through adsorption on the surface. Tight-binding modelling and ab initio density functional theory calculations lead to a full description of the band structure, including the gap size and charge transfer mechanisms, highlighting a strong substrate–molecule interaction that drives the system into a metallic behaviour.
Integrative analysis of breast cancer reveals prognostic haematopoietic activity and patient-specific immune response profiles
Authors: Varn Frederick S. , Andrews Erik H. , Mullins David W. , Cheng Chao 
Published: January 2016

AbstractTranscriptional programmes active in haematopoietic cells enable a variety of functions including dedifferentiation, innate immunity and adaptive immunity. Understanding how these programmes function in the context of cancer can provide valuable insights into host immune response, cancer severity and potential therapy response. Here we present a method that uses the transcriptomes of over 200 murine haematopoietic cells, to infer the lineage-specific haematopoietic activity present in human breast tumours. Correlating this activity with patient survival and tumour purity reveals that the transcriptional programmes of many cell types influence patient prognosis and are found in environments of high lymphocytic infiltration. Collectively, these results allow for a detailed and personalized assessment of the patient immune response to a tumour. When combined with routinely collected patient biopsy genomic data, this method can enable a richer understanding of the complex interplay between the host immune system and cancer.
F-actin mechanics control spindle centring in the mouse zygote
Authors: Chaigne Agathe , Campillo Clément , Voituriez Raphaël , Gov Nir S. , Sykes Cécile , Verlhac Marie-Hélène , Terret Marie-Emilie 
Published: January 2016

AbstractMitotic spindle position relies on interactions between astral microtubules nucleated by centrosomes and a rigid cortex. Some cells, such as mouse oocytes, do not possess centrosomes and astral microtubules. These cells rely only on actin and on a soft cortex to position their spindle off-centre and undergo asymmetric divisions. While the first mouse embryonic division also occurs in the absence of centrosomes, it is symmetric and not much is known on how the spindle is positioned at the exact cell centre. Using interdisciplinary approaches, we demonstrate that zygotic spindle positioning follows a three-step process: (1) coarse centring of pronuclei relying on the dynamics of an F-actin/Myosin-Vb meshwork; (2) fine centring of the metaphase plate depending on a high cortical tension; (3) passive maintenance at the cell centre. Altogether, we show that F-actin-dependent mechanics operate the switch between asymmetric to symmetric division required at the oocyte to embryo transition.
Mechanism of FGF receptor dimerization and activation
Authors: Sarabipour Sarvenaz , Hristova Kalina 
Published: January 2016

AbstractFibroblast growth factors (fgfs) are widely believed to activate their receptors by mediating receptor dimerization. Here we show, however, that the FGF receptors form dimers in the absence of ligand, and that these unliganded dimers are phosphorylated. We further show that ligand binding triggers structural changes in the FGFR dimers, which increase FGFR phosphorylation. The observed effects due to the ligands fgf1 and fgf2 are very different. The fgf2-bound dimer structure ensures the smallest separation between the transmembrane (TM) domains and the highest possible phosphorylation, a conclusion that is supported by a strong correlation between TM helix separation in the dimer and kinase phosphorylation. The pathogenic A391E mutation in FGFR3 TM domain emulates the action of fgf2, trapping the FGFR3 dimer in its most active state. This study establishes the existence of multiple active ligand-bound states, and uncovers a novel molecular mechanism through which FGFR-linked pathologies can arise.
Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-through
Authors: Batista Luciana , Bourachot Brigitte , Mateescu Bogdan , Reyal Fabien , Mechta-Grigoriou Fatima 
Published: January 2016

AbstractThe miR-200 family members have been implicated in stress responses and ovarian tumorigenesis. Here, we find that miR-200c/141 transcription is intimately linked to the transcription of the proximal upstream gene PTPN6 (SHP1) in all physiological conditions tested. PTPN6 and miR-200c/141 are transcriptionally co-regulated by two complementary mechanisms. First, a bypass of the regular PTPN6 polyadenylation signal allows the transcription of the downstream miR-200c/141. Second, the promoters of the PTPN6 and miR-200c/141 transcription units physically interact through a 3-dimensional DNA loop and exhibit similar epigenetic regulation. Our findings highlight that transcription of intergenic miRNAs is a novel outcome of transcriptional read-through and reveal a yet unexplored type of DNA loop associating two closely located promoters. These mechanisms have significant relevance in ovarian cancers and stress response, pathophysiological conditions in which miR-200c/141 exert key functions.
Impact of multiple bird partners on the seed dispersal effectiveness of China’s relic trees
Authors: Li Ning , Li Xin-hai , An Shu-qing , Lu Chang-hu 
Published: January 2016

AbstractFrugivorous birds generally exhibit an unequal contribution to dispersal effectiveness of plant species as a function of their habitat adaptation and body size. In our study, we compared the effectiveness of multiple bird species that contribute to the dispersal of the endangered relic Chinese yew, Taxus chinensis. Seven bird species dispersed T. chinensis seeds, with Picus canus, Turdus hortulorum and Urocissa erythrorhyncha being the main dispersers. The quantity part of dispersal effectiveness was strongly influenced by two inherent characteristics of disperser species: body size and habitat adaptation. However, the quality part of dispersal effectiveness was only influenced by disperser type. For instance, small generalist birds and large specialist birds removed more seeds than other type dispersers. Moreover, small birds and specialist birds contributed slightly more to the dispersal quality of T. chinensis than large birds and generalist birds respectively; however, these differences were not significant. Our results suggest that dispersal effectiveness is affected by variety in the body size and habitat adaptation of different dispersers. Therefore, such variation should be incorporated into spatial and temporal management actions of relic plant species in patchy, human-disturbed habitats.
Evaluation of force generation mechanisms in natural, passive hydraulic actuators
Authors: Le Duigou A. , Castro M. 
Published: January 2016

AbstractPine cones are well known natural actuators that can move their scales upon humidity gradient. The mechanism manifests itself through a displacement easily observable by the naked eye, but coupled with stress generation. In ancient Egypt, wooden wedges were used to break soft blocks of stone by the generated swelling stress. The purpose of the present study is to evaluate the ability of pine cone scales to generate forces while being wetted. In our experiments, a blocking force of around 3N is measured depending on the position on the pine cone where the scales are extracted. A fairly good agreement is obtained when theoretical results based on bimetallic strip systems are compared with experimental data, even if overestimation is observed arising from the input data considered for dry tissues. Inspired by a simplified pine cone microstructure, a biocomposite analogue is manufactured and tested. Although an adequate blocking force can be generated, it has a lower value compared to natural pine cones which benefit from optimized swelling tissue content and interfacial bond strength between them. This study provides new insights to understand the generation of force by pine cones as well as to develop novel biocomposite functionalities.
Dissecting the structural basis of MEIG1 interaction with PACRG
Authors: Li Wei , Walavalkar Ninad M. , Buchwald William A. , Teves Maria E. , Zhang Ling , Liu Hong , Bilinovich Stephanie , Peterson Darrell L. , Strauss III Jerome F. , Williams Jr David C. , Zhang Zhibing 
Published: January 2016

AbstractThe product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail.
Energy efficient reduced graphene oxide additives: Mechanism of effective lubrication and antiwear properties
Authors: Gupta Bhavana , Kumar N. , Panda Kalpataru , Dash S. , Tyagi A. K. 
Published: January 2016

AbstractOptimized concentration of reduced graphene oxide (rGO) in the lube is one of the important factors for effective lubrication of solid body contacts. At sufficiently lower concentration, the lubrication is ineffective and friction/wear is dominated by base oil. In contrast, at sufficiently higher concentration, the rGO sheets aggregates in the oil and weak interlayer sliding characteristic of graphene sheets is no more active for providing lubrication. However, at optimized concentration, friction coefficient and wear is remarkably reduced to 70% and 50%, respectively, as compared to neat oil. Traditionally, such lubrication is described by graphene/graphite particle deposited in contact surfaces that provides lower shear strength of boundary tribofilm. In the present investigation, graphene/graphite tribofilm was absent and existing traditional lubrication mechanism for the reduction of friction and wear is ruled out. It is demonstrated that effective lubrication is possible, if rGO is chemically linked with PEG molecules through hydrogen bonding and PEG intercalated graphene sheets provide sufficiently lower shear strength of freely suspended composite tribofilm under the contact pressure. The work revealed that physical deposition and adsorption of the graphene sheets in the metallic contacts is not necessary for the lubrication.