bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplexed panel of precisely quantified salivary proteins for biomarker assessment
Authors: Percy Andrew J. , Hardie Darryl B. , Jardim Armando , Yang Juncong , Elliott Monica H. , Zhang Suping , Mohammed Yassene , Borchers Christoph H. 
Published: August 2016

An increasingly popular “absolute” quantitative technique involves the SRM or MRM approach with stable isotope‐labeled standards (SIS). Using this approach, many proteins in human plasma/serum have been quantified for biomarker assessment and disease stratification. Due to the complexity of plasma and the invasive nature of its collection, alternative biosamples are currently being explored. Here, we present the broadest panel of multiplexed MRM assays with SIS peptides for saliva proteins developed to date. The validated panel consists of 158 candidate human saliva protein biomarkers, inferred from 244 interference‐free peptides. The resulting concentrations were reproducibly quantified over a 6 order‐of‐magnitude concentration range (from 218 μg/mL to 88 pg/mL; average CVs of 12% over analytical triplicates). All concentrations were determined from reverse standard curves, which were generated using a constant concentration of endogenous material with varying concentrations of spiked‐in SIS peptides. The large‐scale screening of the soluble and membrane‐associated proteins contained within the 158‐plex assay could present new opportunities for biomarker assessment and clinical diagnostics.
Changes in Lymphangiogenesis and Vascular Endothelial Growth Factor Expression by Neo‐Adjuvant Hormonal Therapy in Prostate Cancer Patients
Authors: Asai Akihiro , Miyata Yasuyoshi , Matsuo Tomohiro , Shida Yohei , Hakariya Tomoaki , Ohba Kojiro , Sakai Hideki 
Published: August 2016

BACKGROUNDThe anti‐cancer mechanism of neo‐adjuvant hormonal therapy (NHT) is not well understood. Lymphangiogenesis plays an important role in cancer progression and is regulated by a complex mechanism that includes vascular endothelial growth factor (VEGF) signaling. However, there is little information regarding relationship between lymphangiogenesis and androgen deprivation. The aim of this study was to clarify changes in lymphangiogenesis and VEGF expression induced by androgen deprivation in prostate cancer in vivo and in vitro.METHODSPatients who had undergone a radical prostatectomy were enrolled in the study (NHT, n = 60 and non‐NHT, n = 64). Lymph vessels were identified by D2‐40 immunoreactivity and lymph vessel density and lymph vessel area (LVD and LVA, respectively) were measured from micrographs. The expression of VEGF‐A, ‐B, ‐C, and ‐D was evaluated by immunohistochemistry. The prognostic value of LVD and LVA for biochemical recurrence was also investigated.RESULTSMean LVD ± SD was higher in the NHT than in the non‐NHT group (11.3 ± 3.0 vs. 7.1 ± 3.4 per high power field; P < 0.001). LVA was larger in the NHT than in the non‐NHT group (512.8 ± 174.9 vs. 202.7 ± 72.8 µm2; P < 0.001). VEGF‐A expression was lower whereas VEGF‐C and ‐D levels were higher in the NHT than in the non‐NHT group. VEGF‐B expression in specimens with NHT was lower than that in biopsy specimens at diagnosis. These results were confirmed by in vitro studies used androgen‐sensitive prostate cancer cell line. LVA was found to be an independent predictor of biochemical recurrence in patients who received NHT.CONCLUSIONSOur results demonstrate that NHT stimulates lymphangiogenesis via upregulation of VEGF‐C and ‐D, which may increase LVA and affect the outcome of prostate cancer patients. This findings were supported by in vitro data of prostate cancer cell. Prostate 77:255–262, 2017. © 2016 The Authors. The Prostate Published by Wiley Periodicals, Inc.
Pathogenic nature of Syncephalastrum in Atta sexdens rubropilosa fungus gardens
Authors: Barcoto Mariana O , Pedrosa Felipe , Bueno Odair C , Rodrigues Andre 
Published: August 2016

AbstractBACKGROUNDLeaf‐cutter ants are considered to be a major herbivore and agricultural pest in the Neotropics. They are often controlled by environmentally persistent insecticides. Biological control using pathogenic fungi is regarded as an alternative for the management of these insects. Here, we assess whether the filamentous fungus Syncephalastrum sp. is a pathogenic microorganism responsible for a characteristic disease in fungus gardens. We also characterise the damage caused by this fungus by evaluating physiological and behavioural responses of Atta sexdens rubropilosa subcolonies infected with Syncephalastrum sp.RESULTS:Syncephalastrum sp. fulfils Koch's postulates characterising it as a pathogenic microorganism. Ant workers recognise the infection and remove contaminated fragments from the fungus garden. Syncephalastrum sp. infection causes an interruption of foraging activity, an increase in ant mortality, subcolony deterioration and an increase in the amount of waste generated, all resulting in subcolony death. Syncephalastrum sp. also inhibits the ant fungal cultivar in vitro. The pathogenic effect of Syncephalastrum sp. does not depend on host morbidity or stress (e.g. worker mortality caused by an entomopathogenic fungus).CONCLUSIONSyncephalastrum sp. treatment resulted in progressive damage in subcolonies. The interactions among Syncephalastrum sp., fungus garden and ants offer new opportunities in integrated pest management of leaf‐cutter ants. © 2016 Society of Chemical Industry
Positron annihilation lifetime in float‐zone n‐type silicon irradiated by fast electrons: a thermally stable vacancy defect
Authors: Arutyunov Nikolay , Emtsev Vadim , Krause‐Rehberg Reinhard , Elsayed Mohamed , Oganesyan Gagik , Kozlovskii Vitalii 
Published: August 2016

AbstractTemperature dependency of the average positron lifetime has been investigated for n‐type float‐zone silicon, n‐FZ‐Si(P), subjected to irradiation with 0.9 MeV electrons at RT. In the course of the isochronal annealing a new defect‐related temperature‐dependent pattern of the positron lifetime spectra has been revealed. Beyond the well known intervals of isochronal annealing of acceptor‐like defects such as E‐centers, divacancies and A‐centers, the positron annihilation at the vacancy defects has been observed in the course of the isochronal annealing from ∼ 320 °C up to the limit of reliable detecting of the defect‐related positron annihilation lifetime at ≥ 500 °C. These data correlate with the ones of recovery of the concentration of the charge carriers and their mobility which is found to continue in the course of annealing to ∼ 570 °C; the annealing is accomplished at ∼650 °C. A thermally stable complex consisting of the open vacancy volume and the phosphorus impurity atom, Vop‐P, is suggested as a possible candidate for interpreting the data obtained by the positron annihilation lifetime spectroscopy. An extended couple of semi‐vacancies, 2Vs‐ext, as well as a relaxed inwards a couple of vacancies, 2Vinw, are suggested as the open vacancy volume Vop to be probed with the positron. It is argued that a high thermal stability of the Vs‐ext PVs‐ext (or VinwPVinw.) configuration is contributed by the efficiency of PSi5 bonding. (© 2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular‐sized capillary
Authors: Yin Yue , Li Gongyu , Guan Yafeng , Huang Guangming 
Published: August 2016

RationaleThe flow rate match has been a great challenge when coupling capillary electrophoresis (CE) with electrospray ionization mass spectrometry (ESI‐MS). Conventional CE‐ESI‐MS interfaces used liquid sheath flow, narrowed capillary or additional pressure to meet this requirement; sacrifice of either capillary inner diameter (i.d.) or separation efficiency is often inevitable. Thus, a regular‐sized capillary‐based sheathless interface would be attractive for flow rate match in CE‐MS.MethodsThe regular‐sized capillary‐based CE‐MS interface was achieved by coupling CE with induced electrospray ionization (iESI) which was stimulated by the fact that the iESI could both achieve flow rate down to 0.2 μL/min and retain ionization efficiency. The CE‐iESI‐MS interface was completed with an intact separation capillary, outside the outlet end of which a metal electrode was attached for the application of alternating current (ac) high voltage (HV).ResultsThe feasibility of this CE‐iESI‐MS interface was demonstrated through the stable total ion chromatograms obtained by continuous CE infusion of tripropylamine with regular‐sized capillaries. Tripropylamine and atenolol were separated and detected successfully in phosphate buffer solution (PBS) by CE‐iESI‐MS using a 50 or 75 μm i.d. capillary. Furthermore, this new interface showed a better signal‐to‐noise (S/N) of 3 to 7 times enhancement compared with another sheathless CE‐ESI‐MS interface that using one high voltage for both separation and electrospray when analyzing the mixture of tripropylamine and proline in NH4OAc buffer. In addition, the reproducibility of this interface gave satisfactory results with relative standard deviation (RSD) in retention time in the range between 1% and 3%.ConclusionsThe novel sheathless CE‐MS interface introduced here could match conventional electroosmotic flow (EOF) with electrospray which could also preserve the separation efficiency and sensitivity of CE‐MS. This newly developed CE‐iESI‐MS interface was also demonstrated to be effective for different buffers, PBS and NH4OAc, without any additives such as methanol and acetic acid. Hence, we believe that this sheathless CE‐MS interface could be operated with other nonvolatile and volatile buffers. Copyright © 2016 John Wiley & Sons, Ltd.
Identification of single amino acid substitutions (SAAS) in neuraminidase from influenza a virus (H1N1) via mass spectrometry analysis coupled with de novo peptide sequencing
Authors: Peng Qisheng , Wang Zijian , Wu Donglin , Li Xiaoou , Liu Xiaofeng , Sun Wanchun , Liu Ning 
Published: August 2016

RationaleAmino acid substitutions in the neuraminidase of the influenza virus are the main cause of the emergence of resistance to zanamivir or oseltamivir during seasonal influenza treatment; they are the result of non‐synonymous mutations in the viral genome that can be successfully detected by polymer chain reaction (PCR)‐based approaches. There is always an urgent need to detect variation in amino acid sequences directly at the protein level. Mass spectrometry coupled with de novo sequencing has been explored as an alternative and straightforward strategy for detecting amino acid substitutions, as well – this approach is the primary focus of the present study.MethodsInfluenza virus (A/Puerto Rico/8/1934 H1N1) propagated in embryonated chicken eggs was purified by ultracentrifugation, followed by PNGase F treatment. The deglycosylated virion was lysed and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE). The gel band corresponding to neuraminidase was picked up and subjected to liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis.ResultsLC–MS/MS analyses, coupled with manual de novo sequencing, allowed the determination of three amino acid substitutions: R346K, S349 N, and S370I/L, in the neuraminidase from the influenza virus (A/Puerto Rico/8/1934 H1N1), which were located in three mutated peptides of the neuraminidase: YGNGVWIGK, TKNHSSR, and PNGWTETDI/LK, respectively.ConclusionsWe found that the amino acid substitutions in the proteins of RNA viruses (including influenza A virus) resulting from non‐synonymous gene mutations can indeed be directly analyzed via mass spectrometry, and that manual interpretation of the MS/MS data may be beneficial. Copyright © 2016 John Wiley & Sons, Ltd.
Linear ion trap mass selectivity with impulse power supply and sinusoidal dipolar excitation
Authors: Xu Fuxing , Konenkov Nikolai V. , Ding Hangyu , Wang Yuanyuan , Ding Chuan‐Fan 
Published: August 2016

RationaleAn ion trap mass analyzer can be operated by either a sinusoidal waveform power supply or an impulse waveform power supply. The optimal conditions for the performance of ion trap which is driven by an impulse waveform power supply with sinusoidal dipolar voltage were investigated theoretically, and further verified by experiments.MethodsThe analytical relationship between β and q values is derived theoretically for optimal performance, and the dependencies β(q) for different trapezoidal waveforms are studied. To explain the dependence of resolution with working point q, the derivative dβ/dq is also derived analytically for the case of a rectangular waveform power supply. The theoretical results are further verified by experiments.ResultsThe results from both theoretical calculations and experiments are in very good agreement. The behaviour resolution with q is controlled by the dispersion dβ/dq that was also confirmed by experiments, when the resolution increases with q.ConclusionsThe optimal conditions of β, qex values and required excitation time n are when S(q) is close to 1 for an ion trap driven by trapezoidal waveform voltage with sinusoidal dipolar voltage. It shown that with increasing impulse parameter τ the dispersion dβ/dq decreases and the mass resolution also decreases as result. In the case of applying a rectangular waveform shape voltage, the mass selectivity is the same as for the sinusoidal wave shape. Copyright © 2016 John Wiley & Sons, Ltd.
Adaptive stationary consensus protocol for a class of high‐order nonlinear multiagent systems with jointly connected topologies
Authors: Rezaee Hamed , Abdollahi Farzaneh 
Published: August 2016

SummaryThe leaderless consensus problem in a class of dynamic agents with high‐order input‐affine nonlinear models is studied. Based on communication of position information among the agents, an adaptive protocol is proposed that guarantees achieving consensus in the network in the presence of unknown parameters in the agents models. The network topology is considered undirected, which may not be connected constantly. Hence, by invoking the Cauchy convergence criterion, sufficient conditions to achieve consensus in the presence of jointly connected switching topologies are obtained. A numerical example for a team of single‐link flexible joint manipulators with forth‐order nonlinear models is provided to confirm the accuracy of the proposed consensus protocol. Copyright © 2016 John Wiley & Sons, Ltd.
Leakage‐resilient ID‐based signature scheme in the generic bilinear group model
Authors: Wu Jui‐Di , Tseng Yuh‐Min , Huang Sen‐Shan 
Published: August 2016

AbstractSecurity mechanisms in the traditional cryptography have been modeled under the assumption that secret values (keys) are completely hidden to an adversary. Indeed, a number of side‐channel attacks (e.g., timing, power, fault, etc.) have been demonstrated to obtain partial information about secret keys. Leakage‐resilient cryptography is a countermeasure to withstand side‐channel attacks. Recently, a large number of leakage‐resilient cryptographic schemes or protocols have been proposed to resist various side‐channel attacks. Up to now, no work has been done on leakage‐resilient identity‐based signature (LR‐IBS). In this article, we propose the first LR‐IBS scheme based on Galindo and Vivek's leakage‐resilient signature. In the generic bilinear group model, we formally prove that our LR‐IBS scheme possesses existential unforgeability against identity and adaptive chosen message attacks under the continual leakage model. Copyright © 2016 John Wiley & Sons, Ltd.
Cholesterol–based Bisamides on Biphenyl Backbone: A Case of Selective Visual Sensing of F− and H2PO4− through Breaking and Making of Gels
Authors: Ghosh Kumaresh , Panja Santanu 
Published: August 2016

AbstractCholesterol linked bisamides 1–3 on biphenyl spacers have been designed and synthesized. Compound 1 forms gel in CHCl3 – n‐hexane (1:6, v/v) solvent and the gel is selectively collapsed into sol in presence of F− with a color change from yellowish white to greenish yellow. Presence of pyridinium amides in 2 and 3 showed different gelation behaviours. While the chloride salt 2 displayed gelation in CHCl3 in presence of H2PO4−, compound 3 formed gel in DMSO: H2O (1:2, v/v) without having any assistance of externally added anion. The gel derived from 3 underwent rapid gel to sol transition in presence of H2PO4− and F− anions and validated their visual sensing.
Sustainable High‐Temperature Phthalonitrile Resins Derived from Resveratrol and Dihydroresveratrol
Authors: Laskoski Matthew , Clarke Jadah S. , Neal Arianna , Harvey Benjamin G. , Ricks‐Laskoski Holly L. , Hervey W. Judson , Daftary Mehana N. , Shepherd Arica R. , Keller Teddy M. 
Published: August 2016

AbstractThe renewable triphenols, resveratrol and dihydroresveratrol, were used in the preparation of two new phthalonitrile resins (PN), each containing three phthalonitrile groups. These are the first reported PN resins generated from a renewable feedstock. These amorphous PN resins were characterized thoroughly by DSC, NMR spectroscopy and rheometry. The monomers exhibited excellent rheometric viscosities suitable for resin transfer molding and resin infusion molding. After complete cure, the PN polymers exhibited thermal stability to above 500 °C and no glass transition temperature (Tg). As a result of the highly cross‐linked nature of the network, these materials exhibited a low water uptake of 1 ± 0.2 % and dielectric constant values of approximately 3.0. These results suggest that resveratrol‐derived PN resins are excellent candidates for use in maritime environments and aerospace applications.
Xanthenedione Substituted Metallophthalocyanines as an Efficient and Recyclable Catalyst for One–pot Three Component Synthesis of 3‐Substituted Indoles
Authors: Yadav Kumar Karitkey , Ahmad Sohail , Narang Uma , Bhattacharya Soumee , Chauhan S. M. S. 
Published: August 2016

AbstractAn efficient one‐pot methodology for the synthesis of 3‐amino‐alkylated indoles catalyzed by metallophthalocyanines has been developed via a three component Mannich type reaction with secondary amines, aldehydes and indoles. The newer catalysts i. e. metallophthalocyanines were synthesized by tetramerization of xanthenedione substituted phthalonitrile in anhydrous N,N‐dimethylformamide by conventional heating and microwave conditions and were characterized by different spectroscopic techniques. The aggregation behaviors of these metallophthalocyanines were studied at different concentration in dimethyl sulfoxide and it was observed that these metallophthalocyanines show monomeric behavior in DMSO. The catalyst was separated from the reaction medium, characterized and was successfully reused up to five catalytic cycles. It was observed that the catalyst is stable in the catalytic system. Based on observations, a suitable reaction pathway is proposed for the current reaction.
A Terbium‐Organic Framework Material for Highly Sensitive Sensing of Fe3+ in Aqueous and Biological Systems: Experimental Studies and Theoretical Analysis
Authors: Han Min‐Le , Xu Guo‐Wang , Li Dong‐Sheng , Azofra Luis Miguel , Zhao Jun , Chen Banglin , Sun Chenghua 
Published: August 2016

AbstractOwing to the paramount importance of iron in biological and environmental systems, advanced agents towards the detection of iron ions with high sensitivity and selectivity have been targeted for decades.Here a new super water‐stable agent, namely terbium metal‐organic framework (Tb‐MOF), [H2N(CH3)2][Tb(cppa)2(H2‐ O)2],(CTGU‐1, CTGU=China Three Gorges University, H2cppa=5‐(4‐carboxyphenyl)picolinic acid),has been successfully synthesized and demonstrated as a fluorescent turn‐off sensor for highly sensitive detection of Fe3+ with the detection limit of 10−9 M in dimethylformamide (DMF), 10−7 M in water and 10−7 M in biological environment,respectively. Such detecting capacity, to our knowledge, is the best compared to those of reported lanthanide‐baed MOF fluorescent probes so far. With the combination of first principle calculations, the detecting capacity has been analysed from the interaction between metal ions and organic ligands, particularly the effect of metal ions on the frontier molecular orbitals. This work offers a new Ln–MOF probe towards the detection of metal irons with high sensitivity and selectivity.
Electronic Structure Controlling of Assembly and Optical Properties of TiO2 Nanotube Arrays
Authors: Dai Jianhong , Song Yan 
Published: August 2016

AbstractFirst principles calculations are carried out to study the interaction between TiO2 nanotubes. Two type tubes, the (9, 0) and (0, 6) tubes, with different separations and arrangements are studied in detail. Interactions between ions from neighboring nanotubes and contact angle between tubes could greatly affect the stability of nanotube arrays. The (0, 6) nanotubes tend to contact with each other owing to the attracting interactions between Ti and O ions from different tubes, while the (9, 0) nanotubes prefer to distribute separately due to the repulsion between oxygen ions in neighboring nanotubes. The atomic configurations in contact zone and the electronic structures are further studied to clarify the controlling mechanisms of the stability of TiO2 nanotube arrays. Regulation of arrangement of TiO2 nanotube arrays bring diverse electronic and optical properties.
The HR executive effect on firm performance and survival
Authors: Chadwick Clint , Guthrie James P. , Xing Xuejing 
Published: August 2016

Research summary: This article replicates and extends Welbourne and Cyr's (1999) pioneering study of the relationship between the presence of human resource executives (HREs) and firm performance in initial public offering (IPO) firms. We employ a larger, more generalizable sample, a model better fitted to the IPO context, and alternative performance measures to execute a more robust examination of this relationship. Using Welbourne and Cyr's model specifications, our results parallel theirs: there is no significant relationship between HREs and firms' post‐IPO financial performance. Moreover, extending Welbourne and Cyr's study, we find that the presence of HREs at the time of firms' IPOs is positively related to the ultimate success measure: post‐IPO firm survival. Additionally, for firms that had executives at the time of IPO, the negative effect of firm size on firm mortality is greater and the positive effect of leverage on firm mortality is smaller.Managerial summary: Does paying attention to HRM help firms to succeed? This study examines that question in a sample of generally small, young firms that went public from 1996 to 2008. Only about 10 percent of these firms had human resource executives (HREs) at the time of their IPOs, allowing us to examine the effects of the presence of HREs on firm performance. While we find that the presence of an HRE at IPO is not related to firms' post‐IPO financial performance, firms that had HREs at IPO are significantly more likely to survive in the long run. Since approximately 20 percent of the sample firms do not survive, this is a very meaningful outcome. Indeed, survival may be the ultimate measure of firm performance. Copyright © 2016 John Wiley & Sons, Ltd.