bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Human Papillomavirus 33 E2 DNA Binding Domain With Polyphenols: Unveiling Interactions Through Biophysical and In Silico Methods
Authors: Bharti  , Nair Maya S. 
Published: October 2024

ABSTRACTThe human papillomavirus (HPV) 33 is a high‐risk strain that causes lesions with potential cancerous outcomes. Its E2 protein regulates the viral protein transcription and life cycle maintenance. The DNA binding domain (DBD) of the E2 protein plays a crucial role in the viral life cycle. The DBD region of the E2 protein is particularly interesting for targeting and finding potential inhibitors to inhibit its function or dimerization. Given the limited research on HPV 33 and its proteins, the present work delved into the interaction of two natural polyphenolic compounds, resveratrol, and baicalein, with the E2 DBD of HPV 33 using biophysical and in silico studies. Fluorescence studies of the E2 DBD‐polyphenol complexes showed fluorescence quenching with a binding constant of the order of 106 M−1. Circular dichroism data reveal conformational changes upon binding with the polyphenols, possibly due to distinct binding sites of the E2 DBD. Differential scanning calorimetry exhibited higher melting temperatures for the two complexes than alone DBD, suggesting the complexes' stability. ITC experiment suggested favorable binding reactions with k

d
values in the micromolar range. Molecular docking and dynamic simulation studies revealed that the resveratrol binds to the helical region and baicalein near the central dimeric interface of E2 DBD with a good binding affinity, forming a stable protein‐ligand complex during the run of 100 ns simulation. Therefore, the current study identifies both polyphenolic compounds as promising candidates for potential antiviral drug development.
Human mesenchymal stem/stromal cell‐derived extracellular vesicle transport in meniscus fibrocartilage
Authors: Schwartz Gabi , Rana Samir , Jackson Alicia R. , Leñero Clarissa , Best Thomas M. , Kouroupis Dimitrios , Travascio Francesco 
Published: October 2024

AbstractExtracellular vesicles (EVs) derived from endometrial‐derived mesenchymal stem/stromal cells (eMSC) play a crucial role in tissue repair due to their immunomodulatory and reparative properties. Given these properties, eMSC EVs may offer potential benefits for meniscal repair. The meniscus, being partly vascularized, relies on diffusivity for solute trafficking. This study focuses on EVs transport properties characterization within fibrocartilage that remains unknown. Specifically, EVs were isolated from Crude and CD146+ eMSC populations. Green fluorescence‐labeled EVs transport properties were investigated in three structurally distinct layers (core, femoral, and tibial surfaces) of porcine meniscus. Diffusivity was measured via custom fluorescence recovery after photobleaching (FRAP) technique. Light spectrometry was used to determine EVs solubility. Both Crude and CD146+ eMSC EVs exhibited high purity (>90% CD63CD9 marker expression) and an average diffusivity of 10.924 (±4.065) µm²/s. Importantly, no significant difference was observed between Crude and CD146+ eMSC EV diffusivity on the meniscal layer (p > 0.05). The mean partitioning coefficient was 0.2118 (±0.1321), with Crude EVs demonstrating significantly higher solubility than CD146+ EVs (p < 0.05). In conclusion, this study underscores the potential of both Crude and CD146+ eMSC EVs to traverse all layers of the meniscus, supporting their capacity to enhance delivery of orthobiologics for cartilaginous tissue healing.
Development of a novel rabbit model for femoroacetabular impingement through surgically induced acetabular overcoverage
Authors: Zhang Siqi , Gao Guanying , Zhou Xiang , Du Cancan , Zhu Yichuan , He Tong‐Chuan , Xu Yan 
Published: October 2024

AbstractThere is a lack of validated small animal models for femoroacetabular impingement (FAI) that induce intra‐articular lesions and cause osteoarthritis (OA) progression. The gene expression profile of articular cartilage in patients with FAI has not been characterized in animal studies. The purpose of this study is to describe a novel rabbit model for FAI with validated induction of intra‐articular lesions and OA progression and to characterize the gene expression pattern in impinged cartilage using this model. Thirty 6‐month‐old New Zealand White rabbits underwent unilateral endobutton implant placement at the acetabular rim to surgically create overcoverage. Radiological assessment confirmed secure placement of endobutton at the acetabular rim for all operated hips with a mean alteration in lateral center‐edge angle (ΔLCEA) of 16.2 ± 6.6°. Gross inspection revealed secondary cartilage injuries in the anterosuperior region of the femoral head for the operated hips. Cartilage injuries were shown to exacerbate with increased impingement duration, as demonstrated by the modified Outerbridge scores and Mankin scores. Immunostaining and quantitative real‐time polymerase chain reaction revealed elevated expression of inflammatory, anabolic and catabolic genes in impinged cartilage. RNA sequencing analysis of cartilage tissue revealed a distinct transcriptome profile and identified C‐KIT, CD86, and CD68 as central markers. Our study confirmed that the novel rabbit FAI model created acetabular overcoverage and produced articular cartilage injury at the impingement zone. Cartilage from the impingement zone demonstrated a heightened metabolic state, corroborating with the gene expression pattern observed in patients with FAI.
Developing a codebook to characterize barriers, enablers, and strategies for implementing learning health systems from a multilevel perspective
Authors: Shaw Louise , Perrier Meg , Tahmasebian Kasra , Wong Kimberly , Yajnik Pranjali , Zhu Zihan , Lyons Kayley 
Published: October 2024

AbstractIntroductionLearning health systems (LHSs) play a crucial role in improving healthcare delivery and outcomes through continuous learning and data‐driven decision‐making. Implementation of LHSs spans individual, organization, and systemic levels of healthcare. This paper outlines a systematic approach for developing a comprehensive codebook to identify barriers, enablers, and strategies associated with the establishment and operation of LHS from a multilevel perspective.MethodsThe codebook development process was divided into two phases and employed a coding team. Phase 1 involved the synthesis of previous literature, which drove the development of initial codes. Phase 2 included the testing of the codebook with a pilot dataset to derive new codes or iterative refinement, ensuring robustness, and validity.ResultsThe literature search revealed 12 papers that detailed the barriers, enablers, and strategies for LHS implementation. Micro‐level codes were derived from a mixture of existing literature and our pilot dataset. Most meso‐level codes barriers and enablers were derived from the literature, with some subcodes derived from participant interviews. All strategies for implementation at the meso‐level were identified in the literature. At the macro‐level, all codes and subcodes were from the literature.ConclusionsThe codebook contributes to the advancement of implementation science in LHS. The codebook facilitates effective analysis and understanding of the key factors influencing the success of LHS implementation, offering practical insights for policymakers, healthcare practitioners and researchers engaged in the ongoing evolution of LHS.
Single‐cell transcriptome atlas revealed bronchoalveolar immune features related to disease severity in pediatric Mycoplasma pneumoniae pneumonia
Authors: Shen Xiantao , Jin Zhengjiang , Chen Xiaomin , Wang Zhenhui , Yi Lu , Ou Yangwei , Gong Lin , Zhu Chengliang , Xu Guogang , Wang Yi 
Published: October 2024

AbstractThe mechanisms underlying protective immunity in mild Mycoplasma pneumoniae pneumonia (MPP) and the pathogenesis of severe MPP, characterized by dysregulated immune responses, remain unclear. Here, we performed single‐cell RNA sequencing (scRNA‐seq) to profile bronchoalveolar lavage fluid (BALF) samples from 13 healthy donors and 24 hospitalized pediatric patients with MPP, covering both mild and severe cases. Severe MPP patients exhibited high levels of exhausted T cells and M1‐like macrophages, with the exhaustion of T cells attributed to persistent type I interferon signaling and inadequate assistance from CD4+ T cells. Significant cell‐cell interactions between exhausted T cells and programmed death‐ligand 1+ (PD‐L1+) macrophages were detected in severe patients, potentially mediated through inhibitor molecules (e.g., PD1) and their receptors (e.g., PD‐L1), as well as human leukocyte antigen class I molecules and their receptors (e.g., KLRC1/D2), resulting in the dysfunction of anti‐MP immune responses. Mild MPP patients were featured by an increased abundance of neutrophils, coupled with enhanced activation, contributing to protective immunity. Together, our study provides a detailed characterization of the BALF immune landscape in MPP patients, revealing distinct immune characteristics between mild and severe cases, which offers a valuable resource for understanding MPP immunopathogenesis and formulating effective therapeutic strategies.
Antibacterial Capabilities of Metallic Nanoparticles and Influencing Factors
Authors: Girma Abayeneh , Mebratie Gedefaw , Mekuye Bawoke , Abera Birhanu , Bekele Tigabu , Alamnie Getachew 
Published: October 2024

ABSTRACTThe increase of antibiotic resistance in bacteria has become a major concern for successful diagnosis and treatment of infectious diseases. Over the past few decades, significant progress has been achieved on the development of nanotechnology‐based medicines for combating multidrug resistance in microorganisms. Among these, metallic nanoparticles (MNPs) hold great promise in addressing this challenge due to their broad‐spectrum and robust antimicrobial properties. This review illustrates the antibacterial activities of MNPs and further elucidates how different factors including synthesis method, size, shape, surface charge, pH, dose, type of capping or stabilizing agents of MNPs, and Gram‐type of the bacteria, impact their antibacterial activities, which are expected to promote the future development of more potent MNP‐based antibacterial agents.
Prognostic Significance of Early Posttreatment Audiometry in Idiopathic Sudden Sensorineural Hearing Loss
Authors: Sofer Harel , Fisher Ran , Tarnovsky Yehuda , Vofo Gaelle , Kaufmann Michal , Stern Shavit Sagit 
Published: October 2024

AbstractObjectiveThis study aimed to assess the prognostic value of early posttreatment initiation audiometry findings in patients with idiopathic sudden sensorineural hearing loss (ISSHL).Study DesignA retrospective cohort study.SettingThe study was conducted at a single tertiary medical center.MethodsA review was conducted on 299 ISSHL patients treated between 2007 and 2023. Patients underwent audiometry on Days 2 to 3 and 5 to 7 posttreatment initiation. Data on demographics, medical history, audiometry results, and prognosis were collected. Prognosis was categorized based on hearing recovery at 1 year postdiagnosis.ResultsOlder age, worse initial speech reception threshold (SRT), ischemic heart disease, cerebrovascular accidents/transient ischemic attacks, hypertension, and diabetes were associated with a poorer prognosis. Posttreatment initiation audiometry findings on Days 2 to 3 and 5 to 7 significantly correlated with prognosis. Patients with a ≥30% improvement had substantial or complete recovery in over 80% of cases, while those with <10% had <30% recovery. Multivariate analysis identified a significant improvement on days 5 to 7 as an independent predictor of complete recovery (odds ratio = 4.25 [95% confidence interval 1.96‐9.23], P = .0002).ConclusionPosttreatment initiation audiometry findings, particularly on Days 5 to 7, hold significant prognostic value in ISSHL patients. A substantial improvement during this timeframe is strongly associated with favorable outcomes. These findings emphasize the potential of posttreatment initiation audiometry as a valuable tool for clinicians in counseling patients with ISSHL.
Rethinking Measures and Mortality Attribution in Health Care: The ENT Example
Authors: Patel Samip , Olivero Lorenzo , Sinclair De Frias Jorge , Anderson Stephanie S. , Pollock Ben D. , Lee Marian D. , Chirila Razvan M. , Murthy Hemant S. , Guru Pramod K. , Taylor Richard , Moreno Franco Pablo , Menser Terri 
Published: October 2024

AbstractNational performance metrics ultimately enhance patient decision‐making and promote meaningful improvements in health care delivery, which makes having valid and reliable measures essential. This study examined US News and World Report metrics from 2019 to 2012 and used electronic health record data, combined with detailed chart review across 3 in‐system hospitals, to assess the provision of care compared to the attribution of patients assigned to the ear, nose, and throat (ENT) mortality group. Of the initial 47 ENT‐attributed deaths, 23 of those were verified, dimensioning the mortality rate from 1.7% to just 0.8%. These results underscore the necessity of rethinking measures and mortality attribution methodologies to be more accurate. Current methods use Medicare Severity Diagnosis Related Group billing coding to map the attribution. We suggest transitioning away from specialty ranking approaches and towards a procedure and condition “rating” approach to ensure that these ranking types capture data about the provision of care within a given encounter.
High‐Valence Metals Accelerate the Reaction Kinetics for Boosting Water Oxidation
Authors: Li Lun , Qiao Lulu , Liu Di , Yu Zhichao , An Keyu , Yang Jiao , Liu Chunfa , Cao Youpeng , Pan Hui 
Published: October 2024

AbstractThe transition metal with high valence state in oxyhydroxides can accelerate the reaction kinetics, enabling highly intrinsic OER activity. However, the formation of high‐valence transition‐metal ions is thermodynamically unfavorable in most cases. Here, a novel strategy is proposed to realize the purpose and reveal the mechanism by constructing amorphous phase and incorporating of elements with the characteristic of Lewis acid or variable charge state. A model catalyst, CeO2‐NiFeOxHy, is presented to achieve the modulation of valence state of active site (Ni2+→Ni3+→Ni4+) for improved OER, leading to dominant active sites with high valence state. The CeO2‐NiFeOxHy electrode exhibits superior OER performance with overpotential of 214 and 659 mV at 10 and 500 mA cm−2, respectively (without IR correction), and high stability, which are much better than those of NiOxHy, NiFeOxHy and CeO2‐NiOxHy. These findings provide an effective strategy to achieve the active metals with high‐valence state for highly efficient OER.
Novel 2D Material of MBenes: Structures, Synthesis, Properties, and Applications in Energy Conversion and Storage
Authors: Yang Yuquan , Wang Huichao , Wang Chenjing , Liu Jiajia , Wu Hongjing , Liu Naiyan , Wang Qian , Shang Yang , Zheng Jinlong 
Published: October 2024

Abstract2D transition metal borides (MBenes) have garnered significant attention from researchers due to their exceptional electrical conductivity, strong mechanical rigidity, excellent dynamic and thermodynamic stability, which stimulates the enthusiasm of researchers for the study of MBenes. Over the past few years, extensive research efforts have been dedicated to the study of MBenes, resulting in a growing number of synthesis methods being developed. However, there remains a scarcity of comprehensive reviews on MBenes, particularly in relation to the synthesis techniques employed. To address this gap, this review aims to provide a comprehensive summary of the latest research findings on MBenes. An exhaustive exploration of the crystal structure types of MBenes is presented, highlighting the greater structural diversity compared to MXenes. Furthermore, a comprehensive review of the recent advancements in MBenes synthesis methodologies is provided. The review also delves into the physical and chemical properties of MBenes, while elucidating their applications in the realms of energy conversion and energy storage. Lastly, this review concludes by summarizing and offering insights on MBenes from three angles: synthesis, structure‐property relationships, and application prospects.
Selective Void Deposition by Continuous, Ultrathin Ag Film Enabled Stable, High‐Performance AgNWs‐Based Transparent Heaters
Authors: Thi Sanh Vo , Nguyen Thanh Tai , Patel Malkeshkumar , Bhatnagar Priyanka , Choi Chanhyuk , Lee Junghyun , Dao Vinh‐Ai , Kim Joondong 
Published: October 2024

AbstractMetallic nanowire‐based transparent conductors (MNTCs) are essential to various technologies, including displays, heat‐regulating windows, and photo‐communication. Hybrid configurations are primarily adopted to design stable, high‐functioning MNTCs. Although hybrid MNTCs enhance electrical performance, they often suffer from optical degradation due to losses associated with the hybrid layers. Highly conductive hybrid MNTCs with minimal reduction in transparency are achieved with AgNWs/Ag(O)/Al‐doped ZnO (AZO) design. The design provides a high visible light transmittance of 95.1%, representing a minimized optical loss of 3% compared to pristine AgNWs by optimizing optical interference between the AZO and Ag(O) layers. Furthermore, it allows for enhanced mobility of metallic nanowires by controlling the selective formation of conductive layers in the voids of the nanowire networks. The oxygen additive enables a continuous Ag ultrathin film of 6 nm in the macro‐voids of AgNWs system, corresponding to 25 times higher mobility for AgNWs/Ag(O)/AZO than that of sole AgNWs. The significant enhancement in the mobility of AgNWs/Ag(O)/AZO induces a reduction of sheet resistance of MNTCs by 73%. The AgNWs/Ag(O)/AZO, with an optimized sheet resistance of 24 Ω sq−1, is explored for transparent heater applications, demonstrating a fast thermal response with reliable stability, as evidenced by consistent high‐temperature profiles during prolonged operation.
Waste to Wealth: One‐Step Exfoliating of Spent Graphite to Build a Low‐Cost Cathode for Lithium–Sulfur Batteries
Authors: Zhang Qiongfang , Hu Le , Ren Yibin , Li Jianbo , Kong Yuelin , Li Zhen , Huang Yunhui 
Published: October 2024

AbstractWith the booming development of Li‐ion batteries (LIBs), the recycling and reusing of spent graphite (SG) from LIBs is becoming increasingly crucial. Meanwhile, developing low‐cost and efficient carbon hosts for lithium–sulfur (Li–S) batteries has gained widespread attention in the past decade. Nevertheless, the processing of carbon materials as sulfur hosts is often energy‐consuming and complex. Herein, a simple and environmental‐friendly strategy is proposed to reuse the SG to prepare graphene/sulfur composite cathode for Li–S batteries. Due to expanded layer spacing and defects of SG, sulfur molecules can strip it into a graphene‐type host via ball milling. By optimizing the S/SG ratio and ball milling time, the as‐prepared graphene/sulfur composite cathode with 70 wt.% sulfur content exhibits a high capacity of 1000 mAh g−1. With a high sulfur loading of 4.68 mg cm−2, the graphene/sulfur cathode can maintain 526 mAh g−1 after 400 cycles. This work provides a novel waste‐to‐wealth perspective for recycling spent graphite from LIBs to reuse in Li–S batteries.
2D Microporous Polymers/Reduced Graphene Oxide with Built‐In Lithiophilic Sites for Uniform Lithium Deposition in Lithium Metal Anode
Authors: Ghazi Zahid Ali , Yin Lichang , Sun Zhenhua , Sun Chengguo , Shi Yin , Shi Huifa , Li Feng 
Published: October 2024

AbstractLithium (Li) metal is an attractive anode material for use in high‐energy lithium‐sulfur and lithium‐air batteries. However, its practical application is severely impeded by excessive dendrite growth, huge volume changes, and severe side reactions. Herein, a novel Li metal anode composed of lithiophilic two dimensional (2D) conjugated microporous polymer (Li‐CMP) and reduced graphene oxide (rGO) sandwiches (Li‐CMP@rGO) for Li metal batteries (LiMBs) is reported. In the Li‐CMP@rGO anode, the conductive rGO facilitates the charge transfer while the functionalized‐CMP provides Li nucleation sites within the micropores, thereby preventing dendrite growth. As a result, the Li‐CMP@rGO anode can be cycled smoothly at 6 mA cm−2 of current density with a platting capacity of 2 mAh cm−2 for 1000 h. A Coulombic efficiency of 98.4% is achieved over 350 cycles with a low overpotential of 28 mV. In a full cell with LiFePO4 cathode, the Li‐CMP@rGO anode also exhibited good cycling stability compared to CMP@rGO and CMP/Super‐P. As expected, the simulation results reveal that Li‐CMP@rGO has a strong affinity for Li ions compared to CMP@rGO. The strategies adopted in this work can open new avenues for designing hybrid porous host materials for developing safe and stable Li metal anodes.
Subtraction‐based DNA Origami Cryptography by using Structural Defects for Information Encryption
Authors: Jiang Chu , Tan Ruihao , Li Weiying , Zhang Yinan , Liu Huajie 
Published: October 2024

AbstractConventional cryptographic methods rely on increased computational complexity to counteract the threat posed by growing computing power for sustainable protection. DNA cryptography circumvents this threat by leveraging complex DNA recognition to maintain information security. Specifically, DNA origami has been repurposed for cryptography, using programmable folding of the long scaffold strand carrying additional tagged strands for information encryption. Herein, a subtraction‐based cryptographic strategy is presented that uses structural defects on DNA origami to contain encrypted information. Designated staple strands are removed from the staple pool with “hook” strands to create active defect sites on DNA origami for information encryption. These defects can be filled by incubating the structures with the intact pool of biotinylated staple strands, resulting in biotin patterns that can be used for protein‐binding steganography. The yields of individual protein pixels reached over 91%, and self‐correction codes are implemented to aid the information recovery. Furthermore, the encrypted organization of defective DNA origami structures is investigated to explore the potential of this method for scalable information storage. This method uses DNA origami to encrypt information in hidden structural features, utilizing subtraction for robust cryptography while ensuring the safety and recovery of data.
Hydrogen‐Bonded Organic Framework Enhanced Antifouling Property for Efficient In Situ Electrochemical Assay of Cerebral Ascorbic Acid
Authors: Zheng Zhiyuan , Liu Ningxuan , Lu Jiajia , Zhou Xiang , Song Zibin , An Yida , Lu Ling , Zhao Peng , Tao Jia 
Published: October 2024

AbstractAccurate determination of cerebral ascorbic acid (AA) is crucial for understanding ischemic stroke (IS) related pathological events. Carbon fiber microelectrodes (CFEs) have proven to be robust tools with high sensitivity toward AA, however, they face ongoing challenges for in situ measurement due to the non‐specific adsorption of proteins in brain tissue. In this study, the hydrogen‐bonded organic framework PFC‐71 is synthesized and modified on CFEs through π‐π stacking interactions with carboxylated carbon nanotubes (CNT‐COOH). It is found that the gating effect and hydrophilicity of PFC‐71 provided the CFE with excellent antibiofouling properties. As a result, AA exhibited a low oxidation potential of −30 mV on the CFE/CNT‐COOH/PFC‐71, even in the presence of 20 mg mL−1 bovine serum albumin. Given the structural advantages of CFE/CNT‐COOH/PFC‐71, a ratiometric electrochemical strategy for AA is established, enabling the in situ assay of cerebral AA in a middle cerebral artery occlusion (MCAO) model with high accuracy and stability.