bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Light Treatment and Media Combination on Luminescence Endurance of Bioluminescent Bacteria Isolated from Squid [Loligo duvacelli ( D'Orbigny, 1835)]
Authors: Werdani Ika Diana , Choirunnisa Hesti Nur , Sholikah Nur Hidayatus , Prabaningtyas Sitoresmi 
Published: May 2017

<p class="Els-Abstract-text">Some bacteria emits light in the dark. The aim of this research is to find lighting duration and medium composition which produce the most enduring luminescence. The results showed that one of the bacteria isolated from light organ of <em>Loligo duvacelli </em>(D'Orbigny, 1835)<em> </em>squid are able to emit light. The isolation use trisalt solution and cultured into nutrient agar with addition of NaCl emits the longest blue-green light duration under a 1:1 dark-light shift incubation 8.3 d. Meanwhile under a total light, total dark, and in the oven incubator it illuminate for 4.2 d, 3.6 d and 2.6 d, respectively. The combination of beef extract + peptone + a commercial agar + NaCl generate the longest duration of luminescence when incubate in the oven incubator (3,2 d). Meanwhile it illuminated for 2.6 d and 1.7 d on NA + NaCl and NC + NaCl media, respectively, but it failed to illuminate on PCA, NA, and green beans extract + commercial agar + NaCl media. Based on this research we conclude that a commercial agar is potential to replace a technical bacterial agar function.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> bioluminescence; commercial agar; light treatments; <em>Loligo duvaceli</em> (D'Orbigny, 1835); medium treatment.</p></div>
Potency of Endo-Exopolysaccharide from Porphyridium cruentum (S.F.Gray) Nägeli as Antioxidant (DPPH) and Biological Toxicity (BSLT)
Authors: Agustini Ni Wayan Sri , . Kusmiati 
Published: May 2017

<p>Sources of antioxidants are abundant in nature, one of which is derived from microalgae. Microalgae species that have potentially to be developed as a producer of antioxidant compounds is <em>Porphyridium cruentum</em><em> </em>(S.F.Gray) Nägeli. <em>P. cruentum</em> is a red microalga (Rhodophyceae) and has been known as polysaccharides producer (exopolysaccharide and endopolysaccharide) and as an antioxidant. This study aims to determine antioxidant activity of the extracts and biological toxicity of endo- and exopolysaccharide <em>P. cruentum</em>. The antioxidant activity use reduction method of free radicals (DPPH), whereas toxicity uses Brine Shrimp Lethality Test. (BSLT). The result shows that there is an antioxidant activity with IC<sub>50</sub> value of 50.586 5 mg · kg<sup>–1</sup> (exopolysaccharide) and 145.998 8 mg · kg<sup>–1</sup> (endopolysaccharide). In addition, the result indicates the toxic nature of the <em>Artemia salina</em> Leach. with LC<sub>50</sub> values of 513.175 1 mg · kg<sup>–1</sup> (exopolysaccharide) and 521.823 3 mg · kg<sup>–1</sup> (endopolysaccharide). Therefore, endo- and exopolysaccharide produced by <em>P. cruentum</em> suitable to be used as an alternative source of natural antioxidants and potential for further developed as antitumor drugs.</p><div><p class="Els-keywords"><strong>Keywords:</strong><em> </em>antioxidant, biological toxixity, endo-exopolysaccharide, <em>Porphyridium cruentum</em> (S.F.Gray) Nägeli.</p></div>
The Growth and Antioxidative Responses of Sonchus oleraceus (Linnaeus, 1753) Under Cu(II), Pb(II), Cd(II) and Cr(VI) Stress Condition
Authors: Hidayaturrohmah Nur , Kasmiyati Sri , . Sucahyo , Vincēviča-Gaile Zane 
Published: May 2017

<p class="Els-Abstract-text">Plants growing in soil containing heavy metal polutan such as chromium (Cr), lead (Pb), cadmium (Cd) and copper (Cu) will be stunted, and increase production of Reactive Oxygen Species (ROS). In dealing with the excess amount of ROS, plants have an enzymatic defense system, using superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). The aim of this study was to determine and analyze the <em>Sonchus oleraceus</em> (Linnaeus, 1753) plant response to heavy metals stress, seen from the growth and antioxidative defense enzymatically. Research carried out using a Completely Randomized Design (CRD) with four treatments and five replicates. The metal treatment was 10 mg · L<sup>–1</sup>. The presence of heavy metals in the growing medium significantly decreased the plant height and leaf area, so the impact is on the weight of wet and dry weight. The metal treatments of Cr, Cd, Pb and Cu increase the activity of SOD and APX enzymes but decrease the activity of the CAT enzyme. Chromium is a metal that has a significant influence on the growth and activity of SOD, APX, and CAT enzymes in <em>S. oleraceus</em>.</p><div><p class="Els-keywords"><strong>Keywords:</strong> antioxidative responses; growth; heavy metal; <em>Sonchus oleraceus; </em>toxicity.</p></div>
Production and Characterization of Extracellular Protease from Bacillus sp. 140-B Isolated from Pineapple Plantation in Lampung, Indonesia
Authors: Laili Nur , Antonius Sarjiya 
Published: May 2017

<p class="Els-Abstract-text"><em>Bacillus </em>sp<em>.</em> being industrially important organisms produces a wide variety of extracellular enzymes including protease. <em>Bacillus </em>sp. 140-B isolated from rhizosphere area of pineapple plantation in Lampung Province, Indonesia was tested for production of protease on skim milk agar medium. The aims of this study were to characterize and investigate some properties of protease activity from <em>Bacillus </em>sp<em>. </em>140-B. <em>Bacillus </em>sp<em>. </em>140-B showed protease activity qualitatively by clear zone diameter of 15.0 mm and the highest protease activity was 35.02 Unit · mg<strong><sup>–1</sup></strong> protein at 14 h after incubation. Some properties of protease activities from <em>Bacillus </em>sp<em>. </em>140-B including effects of temperature, pH and several metals were observed in this experiment. The protease activity from <em>Bacillus </em>sp<em>. </em>140-B had optimum pH of 7,0 and the optimum temperature was 60 °C. Several metals which were evaluated on protease activity showed that Mn could increase protease activity, while other metals (Ca, Co, and Hg) showed decreasing protease activity of <em>Bacillus </em>sp<em>. </em>140-B.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords: </strong><em>Bacillus sp.</em> 140-B; enzyme activity; enzyme properties; protease</p></div>
Gene Encoding Nitrilase from Soil Sample of Lombok Gold Mine Industry using Metagenomics Approach
Authors: Riffiani Rini , Sulistinah Nunik , Sunarko Bambang 
Published: May 2017

<p class="Els-body-text">This paper describes a efficient screening gene nitrilase from contaminated soil from Lombok gold mine industry. DNA was extracted directly from soil using the soil DNA isolation kit based on enzymatic, chemical and mechanical lysis. The existence of nitrilase gene in soil sample can be identified by nitrilase gene amplification using H1F˗H1R primer. BLASTN analysis result revealed that the nitrilase gene fragment which was amplified by H1F˗H1R primer has a high homology with <em>Rhodococcus rhodochrous </em>strain<em> tg1˗A6 nitrilase gene</em>. These amplification and DNA fragment sequencing results indicated that nitrilase gene existence on soil sample can be identified by metagenomic approach</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> Metagenomics; nitrilase gene; <em>Rhodococcus rhodochrous</em>; soil.</p></div>
Synthesis of cDNA Pun1 gene from Capsicum frutescens L. cv. Cakra Hijau
Authors: Zairina Shelly , Khasna Elhah Nailul , Juliandari Ria Reinnata , Sulasmi Eko Sri , Listyorini Dwi 
Published: May 2017

<p class="Els-Abstract-text"><em>Capsicum frutescens</em> L. cv. Cakra Hijau is a local cultivar that has been widely cultivated in Indonesia due to the pungency. Pungent on <em>Capsicum</em> is generated by capsaicin compound encoded by <em>Pun1</em> gene. The sequences of <em>Pun1 </em>gene containing with two exons that located on the upstream and downstream, which are separated by introns in the middle. This study aimed to synthesis of cDNA of <em>Pun1</em> gene from isolated total mRNA using two primers: F1/R1 (<strong><em>F1 </em></strong>5’- ATG GCT TTT GCA TTA CCA TCA -3’; and <strong><em>R1 </em></strong>5’-CTT AGC TCG AAG TGC ATC TA-3’) to synthesis the exon-1 sequences and F2/R2 (<strong><em>F2 </em></strong>5’-GAA GGT GGC AGA AGA ATC AG-3’; and <strong><em>R2 </em></strong>5’-TTA GGC AAT GAA CTC AAG GA-3’) to synthesis the exon-2 sequences. The cDNAs resulted from RT-PCR were visualized on 1.5 % agarose gel electrophoresis. From this study we obtained a 1323 bp fragment cDNA.</p><p> </p><p><strong>Keywords: </strong><em>Capsicum frutescens</em><em> </em>L. cv<em>.</em>Cakra Hijau; <em>Pun1 </em>gene; synthesis of cDNA.</p>
The Processing Model in Making Tempeh Benguk [Mucuna pruriens (L.) DC] Containing High L-Dopa
Authors: Winarni Sri , Dharmawan Yudhy 
Published: May 2017

<p>“<em>Koro benguk</em>” [<em>Mucuna pruriens</em><em> </em>(<a title="Carolus Linnaeus" href="https://id.wikipedia.org/wiki/Carolus_Linnaeus">L.</a>) <a title="A. P. de Candolle (halaman belum tersedia)" href="https://id.wikipedia.org/w/index.php?title=A._P._de_Candolle&amp;action=edit&amp;redlink=1">DC</a>] seed contains L-dopa which is able to repair spermatozoa quality. The aim of this experiment is to analyze L-Dopa using HPLC in engineering manufacture of tempeh benguk. There are four engineering model’s variants of making tempeh benguk. The engineering process begins with selection of <em>koro benguk </em>seed, tools required, immersion, boiling, humidity, temperature and pH. The result shows that the process in making tempeh benguk which contains the highest concentration of L-Dopa (11 346.85 mg · kg<sup>–1</sup>) is white-colored and intact <em>koro benguk</em> seed; one time immersion with replacing the water for 4 to 5 times/day (two days and two nights); one time boiling (&lt; 1 h until <em>koro benguk</em> seed can be opened); mixed with 10 mL lime juice/1.5 kg, with concentration of 0.83 % until pH 5, added with mashed teak leaves, and ended with the process of making tempeh benguk at room temperature (30 ºC), relative humidity room (65 %). Tempeh <em>benguk </em>which is produced is cleaner, whiter, nonperishable, and more tender.</p><div><p class="Els-keywords"><strong>Keywords:</strong> HPLC; L-dopa; modified process of making; seeds benguk [<em>Mucuna pruriens</em><em> </em>(<a title="Carolus Linnaeus" href="https://id.wikipedia.org/wiki/Carolus_Linnaeus">L.</a>) <a title="A. P. de Candolle (halaman belum tersedia)" href="https://id.wikipedia.org/w/index.php?title=A._P._de_Candolle&amp;action=edit&amp;redlink=1">DC</a>]; tempeh.</p></div>
Molecular Identification of Indigenous Lactic Acid Bacteria, Partial Purification and Characterization of β-Galactosidase Produced
Authors: Khusniati Tatik , . Sulistiania , Pamela Fury Bellen Nastitie , Falah Syamsul , Choliq Abdul 
Published: May 2017

<p>β-Galactosidase is enzyme which hydrolyze lactose to glucose and galactose, as lactose hydrolyzer. To know indigenous lactic acid bacteria (LAB) characteristics, indigenous LAB identification, partial purification and characterization of β-galactosidase produced was researched. LAB was molecularly identified, β-galactosidase partial purification was conducted by precipitation followed dialysis. β-Galactosidase characterization was based on optimum activities of pH and temperature. The results show that LAB was identified as <em>Lactobacillus plantarum</em> B123. Optimum activity of precipited β-galactosidase was reached at 50 % ammonium sulphate. Activity and specific activity of 50 % precipited β-galactosidase were 95.675 U · mg<sup>–1</sup> and 32.268 U · mg<sup>–1</sup>, respectively. Precipited β-galactosidase resulted a purification level of 3.99 fold, a yield of 38.73 %, and a specific activity of 32.27 U · mg<sup>–1</sup> protein, while dialyzed β-galactosidase resulted 7.61 fold, 10.67 %, and 61.53 U· mg<sup>–1</sup> protein. Optimum temperature and pH for crude β-galactosidase were found at 55 °C and 7.0, respectively, while that dialyzed β-galactosidase were optimized at 50 °C and 7.0. Based on partial purification and characterization, <em>Lactobacillus plantarum</em> B123 is indigenous LAB which good for production of β-galactosidase.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> characterization; dialysis; lactic acid bacteria; <em>Lactobacillus plantarum</em> B 123; β-galactosidase.</p></div>
Secondary Metabolites Production by Actinomycetes and their Antifungal Activity
Authors: Dewi Tirta Kumala , Agustiani Dwi , Antonius Sarjiya 
Published: May 2017

<p class="Els-Abstract-text">Wilt desease of banana caused by <em>Fusarium oxysporum</em> f.sp. <em>cubense</em> (FOC) is one of the most destructive deseases of banana in the tropics. Actinomycetes are the most economically and biotechnologically valuable prokaryotes able to produce wide range of bioactive secondary metabolites. The aims of the present study are to isolate and screen the actinomycetes with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, <em>Fusarium oxysporum</em> f. sp. <em>cubense</em>. Two isolates from Lampung and Cianjur showed activity against fungi. The isolates designed as L.3.1 and CiIA5b. The metabolites from potent stain was produced by extraction of culture filtrate with ethyl acetate : methanol (4:1), it was tested for their antifungal activity by well diffusion method. Evidence for in vitro antibiosis of L.3.1 and CiIA5b isolates was demonstrated by the zone of fungal-growth inhibition. Production of secondary metabolites was analysis by thin layer chromatography (TLC) and bioautography assays. In this study, the metabolites from L.3.1 and CiIA5b have showed good antifungal activity.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> Actinomycetes; antifungal activity; bioautography; secondary metabolites; thin layer chromatography.</p></div>
The Effect of Papaya (Carica papaya L.) Leaf Extract to the Number of Trypanosoma evansi Steel in Liver and Kidney of Mice (Mus musculus L. 1758)
Authors: Yahya Yudi , Nurliani Anni , Santoso Heri Budi 
Published: May 2017

<p class="Els-Abstract-text">The purpose of this study proved the activity of the ethanolic extract of papaya leaf as anti-trypanosomiasis agent, determined the dose of extract which having an effect in inhibiting growth of <em>T. evansi</em>, and evaluated the histopathology of liver and kidney infected by <em>T</em><em>rypanosoma evansi</em> Steel. Male Balb-c mice were infected by 3 × 10<sup>7</sup> <em>T. evansi</em> isolates. The native method was used to determine the level of parasitemia.The preparation was made using cytological smear method and paraffin method. The results showed that ethanolic extract of papaya leaf decreased the number of <em>T. evansi</em>. Ethanolic extract of papaya leaf at a dose of 300 mg · kg<sup>–</sup><sup>1</sup> BW is the most influential dose to reduce the number of <em>T. evansi</em> in liver and kidney of mice. Liver reveals degenerative change varying such as congestion, lipid degeneration, vacuolar degeneration, necrosis, and cellular infiltration. Kidney reveals tubular degeneration, necrosis, cellular infiltration, and glomerulonephritis.</p><p class="Els-Abstract-text"> </p><p><strong>Keywords:</strong> <em>Carica papaya</em> L.; kidney; liver; mice; <em>Trypanosoma evansi</em> Steel</p>
Mus musculus (Linnaeus, 1758) Immune Responses Caused by Escherichia coli (Migula, 1895) Infection
Authors: Mitasari Zuni , Gofur Abdul , Listyorini Dwi 
Published: May 2017

<p><em>Escherichia coli</em> (<a title="Walter Migula" href="https://en.wikipedia.org/wiki/Walter_Migula">Migula</a>, 1895) is a negative gram bacteria which have an ability to cause some deseases, such as meningitis, urinary tract infection and digestive tract infection. Lipopolysaccharide (LPS) in its cell wall induces immune responses in many ways. The objective of this study was to investigate the effect of <em>E. coli</em> infection on lymphocyte numbers and spleen weight in mice [<em>Mus musculus</em> (Linnaeus, 1758)]. Twenty five male mice were grouped into negative control, positive control group treated with PBS, and three experimental groups injected intraperitonially with <em>E. coli</em> 1.5 × 10<sup>3 </sup>cfu/mL,1.5 × 10<sup>5 </sup>cfu/mL or 1.5 × 10<sup>7 </sup>cfu/mL respectively. About 5 d after the injection, the spleen were collected. Spleen were weighed using digital balance, number of lymphocyte were counted using hemocytometer. <em>E. coli</em> infection significantly increase both weight of the spleen and number lymphocyte. In conclusion, mice responded to <em>E. coli</em> infection by increasing the number of its lymphocyte.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong><em> Escerechia coli </em>(<a title="Walter Migula" href="https://en.wikipedia.org/wiki/Walter_Migula">Migula</a>, 1895);<em> </em>immune respone; infection; <em>Mus musculus </em>(<a title="Carl Linnaeus" href="https://en.wikipedia.org/wiki/Carl_Linnaeus">Linnaeus</a>, <a title="10th edition of Systema Naturae" href="https://en.wikipedia.org/wiki/10th_edition_of_Systema_Naturae">1758</a>).</p></div>
DIREITOS HUMANOS SOB AMEAÇA: ORGANIZAÇÕES PATOGÊNICAS, TRABALHO E SUBJETIVIDADE
Authors: Silva Eduardo Pinto e , Heloani Roberto , Piolli Evaldo 
Published: May 2017

Objetiva-se, neste artigo, discutir os Direitos Humanos no processo civilizatório atual, e em que medida ele estaria fragilizado, no campo do trabalho, e caracterizado por quantofrenia e desqualificação dos que resistem ou não alcançam metas desumanas de produtividade. Consideramos a universalização dos Direitos Humanos um sinal evidente do processo civilizatório e condição para a democracia e concretização de uma ética balizada na tolerância e prática dialógica. No entanto, ao analisarmos as relações entre direitos humanos, ética e trabalho, nas organizações públicas e educacionais inclusas, e suas influências sobre a subjetividade, apontamos que a barbárie se inscreve no processo civilizatório. De forma contraditória aos princípios éticos e democráticos, são produzidas situações de assédio moral e formas de solidariedade mórbidas no trabalho, calcadas na humilhação e estigmatização.
Palavras-chave: Direitos Humanos. Assédio moral. Ttrabalho. Ssubjetividade. Gerencialismo.
SISTEMA DE ENSINO APOSTILADO: UM “CAVALO DE TROIA”?
Authors: Giacomini Raquel de Melo , Otto Clarícia 
Published: May 2017

Neste artigo, o objetivo é compreender a política utilizada para a implantação do Sistema Educacional Família e Escola (SEFE), em escolas da rede municipal de ensino de Florianópolis (SC), entre 2009 e 2012. O método utilizado foi observação e participação nos cursos de formação continuada de professores, ministrados pelo SEFE, aplicação de 82 questionários e realização de oito entrevistas com professores de escolas que utilizam o SEFE. Como aportes teóricos, tomamos o conceito de estratégias, de acordo com Michel de Certeau; a produção de subjetividades, na acepção de Michel Foucault; e, a governamentalidade neoliberal, conforme indicado por Karla Saraiva e Alfredo Veiga-Neto. Dentre os resultados, inferimos que o SEFE adota estratégias de governamentalidade com características neoliberais, tais como: estímulo à competição, busca pela eficiência e qualidade, cumprimento de metas e práticas de meritocracia. Além disso, é possível identificar que a utilização do material didático do SEFE propicia a emergência de um profissional que atua de forma mecânica, o que pode conduzir a um paulatino processo de perda da autonomia docente.
Palavras-chave: Sistema Educacional Família e Escola. Estratégias. Governamentalidade neoliberal.
O USO DA (AUTO)BIOGRAFIA EM PESQUISAS BRASILEIRAS (2001 – 2010): A CONSOLIDAÇÃO DE UMA TENDÊNCIA METODOLÓGICA
Authors: Da Silva Marilda , Sgobbi Isabela Vicenzo , Carlindo Eva Poliana 
Published: May 2017

O objetivo desta pesquisa é apresentar um mapeamento/balanço quantitativo que possibilita dar visibilidade ao uso que foi feito do recurso teórico-metodológico (auto)biografia por pesquisadores brasileiros no período de 2001 - 2010. Busca-se, assim, mostrar o movimento que consolidou esse recurso como tendência metodológica no referido período no âmbito da história epistemológica produzida em tal tempo no Brasil, nas Ciências Humanas e no Campo Educacional. Trata-se de uma pesquisa documental. As fontes foram 742 pesquisas: 514 mestrados e 228 doutorados. Usou-se o Banco de dados da CAPES. Os dados apontam que o uso do referido recurso metodológico consolidou uma tendência metodológica no âmbito da história epistemológica produzida no Brasil em tal tempo, abarcando as Ciências Humanas de modo geral e o Campo Educacional em particular. Nesse sentido, na década em questão, a média anual de produção foi de 74 pesquisas. Outrossim, os dados evidenciaram os nichos institucionais nucleares da consolidação da tendência.
Palavras-chave: (Auto)biografia. Produção. Ciências Humanas. Campo Educacional.
ASSOCIAÇÃO ENTRE AFETOS E REPRESENTAÇÕES ENVOLVIDAS NO AMBIENTE DE APRENDIZAGEM ESCOLAR
Authors: Osti Andreia , Noronha Ana Paula Porto 
Published: May 2017

O presente estudo parte da premissa de que as dimensões afetivas envolvem o processo de planejamento e desenvolvimento das práticas pedagógicas e constituem fator importante na determinação da natureza das relações estabelecidas entre os estudantes e os diversos objetos de conhecimento. Nesse sentido, objetivou-se analisar como os alunos representam os aspectos afetivos do processo de ensino e aprendizagem e quais suas percepções sobre o ambiente da aprendizagem. Participaram da pesquisa 120 estudantes do 5° ano do ensino fundamental de escolas públicas municipais da Região Metropolitana de Campinas, sendo 60 alunos com satisfatório desempenho acadêmico e 60 com dificuldades de aprendizagem. Metodologicamente foram utilizados três instrumentos: Prova Psicopedagógica Par Educativo, Escala de Afetos Zanon e Escala de Expectativas do Professor. Os resultados revelaram, que alunos com dificuldade de aprendizagem se diferenciam significativamente daqueles com adequado desempenho. Alunos com dificuldades estabelecem menor vínculo com a aprendizagem escolar formal e com seus professores e por isso retratam situações não escolares, enquanto que alunos com desempenho satisfatório têm uma melhor percepção das expectativas de seus professores e demonstram ter uma relação mais afetiva com o ambiente escolar. Acredita-se que essa pesquisa contribui para o entendimento sobre a relação entre os sentimentos vivenciados por alunos no contexto da sala de aula e suas implicações para o desempenho acadêmico dos mesmos.
Palavras-chave: Psicologia positiva. Relações interpessoais. Experiências de aprendizagem.