bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of an End-to-End-Simulator to Analyze CYGNSS
Authors: Hoover Kacie E. , Mecikalski John R. , Lang Timothy J. , Li Xuanli , Castillo Tyler J. , Chronis Themis 
Published: October 2017

AbstractTropical convection during the onset of two Madden–Julian oscillation (MJO) events, in October and December of 2011, was simulated using the Weather Research and Forecasting (WRF) Model. Observations from the Dynamics of the MJO (DYNAMO) field campaign were assimilated into the WRF Model for an improved simulation of the mesoscale features of tropical convection. The WRF simulations with the assimilation of DYNAMO data produced realistic representations of mesoscale convection related to westerly wind bursts (WWBs) as well as downdraft-induced gust fronts. An end-to-end simulator (E2ES) for the Cyclone Global Navigation Satellite System (CYGNSS) mission was then applied to the WRF dataset, producing simulated CYGNSS near-surface wind speed data. The results indicated that CYGNSS could detect mesoscale wind features such as WWBs and gust fronts even in the presence of simulated heavy precipitation. This study has two primary conclusions as a consequence: 1) satellite simulators could be used to examine a mission’s capabilities for accomplishing secondary tasks and 2) CYGNSS likely will provide benefits to future tropical oceanic field campaigns that should be considered during their planning processes.
Short‐term Subjective and Objective Outcomes of Patients Receiving Endoscopic Transcanal Myringoplasty for Repairing Tympanic Perforations
Authors: Tseng Chih‐Chieh , Lai Ming‐Tang , Wu Chia‐Che , Yuan Sheng‐Po , Ding Yi‐Fang 
Published: October 2017

ObjectiveEndoscopic transcanal myringoplasty (ETM) has been an emerging technique for repairing tympanic perforations since the late 1990s. Objective outcomes (ie, graft success rates and hearing results) of patients who received ETM are well documented; however, subjective outcomes of these patients have rarely been reported. Hence, this study evaluated subjective and objective outcomes of patients who received ETM for repairing tympanic perforations.Study DesignProspective study.SettingTertiary care university hospital.Patients and MethodsPatients who underwent ETM for perforations of the tympanic membrane were included. We evaluated subjective variables of patients receiving ETM as the primary outcome and objective variables as the secondary outcome.ResultsIn total, 91 ears that underwent ETM were included. The mean pain scale score was 0.1 (range, 0‐2) on postoperative day 3. The mean duration of pain medication was 2.0 (range, 0‐3) days. The mean number of days required to resume routine activities was 1.0 (range, 1‐2) days. The overall graft success was determined postoperatively at 3 months in 80 of 91 ears (87.9%). Closure of the air‐bone gap to within 20 dB was achieved in 79 (86.8%) ears.ConclusionIn this study, patients who received ETM had mild postoperative pain and resumed routine activities early. These patients also exhibited favorable graft success rates and hearing results at 3 months postoperatively. On the basis of our results, we conclude that patients who receive ETM for the repair of tympanic perforations have favorable short‐term subjective and objective outcomes.
Impact of Resident Participation on Operative Time and Outcomes in Otologic Surgery
Authors: Muelleman Thomas , Shew Matthew , Muelleman Robert J. , Villwock Mark , Sykes Kevin J. , Staecker Hinrich , Lin James 
Published: October 2017

ObjectivesTo describe the impact of resident involvement in tympanoplasty on operative time and surgical complication rates.Study DesignCase series with chart review.SettingTertiary medical center.Subjects and MethodsCurrent Procedural Terminology codes were used to identify patients in the 2011‐2014 public use files of the American College of Surgeons National Surgical Quality Improvement Program who underwent a tympanoplasty or tympanomastoidectomy. Cases were included if the database indicated whether the operating room was staffed with an attending alone or an attending with residents. Categorical and continuous variables were compared with chi‐square, Fisher’s exact, and Mann‐Whitney U tests. Generalized linear models with a log‐link and gamma distribution were used to examine the factors affecting operative time.ResultsOverall, 1045 cases met our study criteria (tympanoplasty, n = 797; tympanomastoidectomy, n = 248). Resident involvement increased mean operative time for tympanoplasties by 46% (107 vs 73 minutes, P <. 001) and tympanomastoidectomies by 49% (175 vs 117 minutes, P <. 001). While controlling for confounding factors, the variable with the largest impact on operative time was resident involvement. There were no significant differences observed in the rate of surgical complications between attending‐alone and attending‐resident cases.ConclusionResident involvement in tympanoplasty and tympanomastoidectomy did not affect the surgical complication rate. Resident involvement increased operative time for tympanoplasties and tympanomastoidectomies; however, the specific reasons for the increase are not explained by the available data.
Pre-season dietary intake of professional soccer players
Authors: Raizel Raquel , da Mata Godois Allan , Coqueiro Audrey Yule , Voltarelli Fabrício Azevedo , Fett Carlos Alexandre , Tirapegui Julio , de Paula Ravagnani Fabricio Cesar , de Faria Coelho-Ravagnani Christianne 
Published: October 2017

Background: Despite the well-documented importance of nutrition in optimizing performance and health, the dietary intake of soccer players has attracted little attention. Aim: We aimed to assess the pre-season dietary intake of professional soccer players and its adequacy in macro and micronutrients. Methods: The pre-season dietary intake of 19 male athletes was assessed using a semi-structured 3-day food record. To determine dietary adequacy and excess, energy and macronutrient intake were compared with the Brazilian dietary reference values for athletes, and micronutrients were compared with the Estimated Average Requirement – EAR (minimum recommendation) and Tolerable Upper Intake Level – UL (maximum recommendation). Results: Mean daily energy intake (40.74±12.81 kcal/kg) was adequate. However, there was a low carbohydrate intake (5.44±1.86 g/kg/day) and a high amount of protein and fat (1.91±0.75 and 1.27±0.50 g/kg/day, respectively). Sodium intake (3141.77±939.76 mg/day) was higher than UL (2300 mg/day), while the majority of players showed daily intake of vitamin A (74%), vitamin D (100%), folate (58%), calcium and magnesium (68%) below the EAR (625, 10 and 320 µg/day, 800 and 330 mg/day, respectively). Conclusion: The dietary intake of professional soccer players was adequate in energy, but inadequate in macro and micronutrients, which suggests the need to improve nutritional practices to sustain the physical demands of soccer during pre-season.
The antidepressant efficacy of subanesthetic-dose ketamine does not correlate with baseline subcortical volumes in a replication sample with major depressive disorder
Authors: Niciu Mark J , Iadarola Nicolas D , Banerjee Dipavo , Luckenbaugh David A , Park Minkyung , Lener Marc , Park Lawrence , Ionescu Dawn F , Ballard Elizabeth D , Brutsche Nancy E , Akula Nirmala , McMahon Francis J , Machado-Vieira Rodrigo , Nugent Allison C , Zarate Carlos A 
Published: October 2017

Background: This study sought to reproduce, in a larger sample, previous findings of a correlation between smaller raw 3-Tesla (3T) hippocampal volumes and improved antidepressant efficacy of ketamine in individuals with major depressive disorder (MDD). A secondary analysis stratified subjects according to functional BDNF rs6265 (val66met) genotype. Methods: Unmedicated subjects with treatment-resistant MDD ( n=55) underwent baseline structural 3T MRI. Data processing was conducted with FSL/FIRST and Freesurfer software. The amygdala, hippocampus, and thalamus were selected a priori for analysis. All subjects received a single 0.5mg/kg × 40-minute ketamine infusion. Pearson correlations were performed with subcortical volumes and percent change in MADRS score (from baseline to 230 minutes, 1 day, and 1 week post-infusion). Results: Raw and corrected subcortical volumes did not correlate with antidepressant response at any timepoint. In val/val subjects ( n=23), corrected left and right thalamic volume positively correlated with antidepressant response to ketamine at 230 minutes post-infusion but did not reach statistical significance. In met carriers ( n=14), corrected left and right thalamic volume negatively correlated with antidepressant response to ketamine. Conclusion: Baseline subcortical volumes implicated in MDD did not correlate with ketamine’s antidepressant efficacy. Baseline thalamic volume and BDNF genotype may be a combinatorial rapid antidepressant response biomarker.
First-in-Man Evaluation of 124I-PGN650: A PET Tracer for Detecting Phosphatidylserine as a Biomarker of the Solid Tumor Microenvironment
Authors: Laforest Richard , Dehdashti Farrokh , Liu Yongjian , Frye Jennifer , Frye Sarah , Luehmann Hannah , Sultan Deborah , Shan Joseph S. , Freimark Bruce D. , Siegel Barry A. 
Published: October 2017

Purpose: PGN650 is a F(ab′)2 antibody fragment that targets phosphatidylserine (PS), a marker normally absent that becomes exposed on tumor cells and tumor vasculature in response to oxidative stress and increases in response to therapy. PGN650 was labeled with 124I to create a positron emission tomography (PET) agent as an in vivo biomarker for tumor microenvironment and response to therapy. In this phase 0 study, we evaluated the pharmacokinetics, safety, radiation dosimetry, and tumor targeting of this tracer in a cohort of patients with cancer. Methods: Eleven patients with known solid tumors received approximately 140 MBq (3.8 mCi) 124I-PGN650 intravenously and underwent positron emission tomography–computed tomography (PET/CT) approximately 1 hour, 3 hours, and either 24 hours or 48 hours later to establish tracer kinetics for the purpose of calculating radiation dosimetry (from integration of the organ time-activity curves and OLINDA/EXM using the adult male and female models). Results: Known tumor foci demonstrated mildly increased uptake, with the highest activity at the latest imaging time. There were no unexpected adverse events. The liver was the organ receiving the highest radiation dose (0.77 mGy/MBq); the effective dose was 0.41 mSv/MBq. Conclusion: Although 124I-PGN650 is safe for human PET imaging, the tumor targeting with this agent in patients was less than previously observed in animal studies.
A systematic review of Federal Drug Administration Docket for community consultation and public disclosure in exception from informed consent trials
Authors: Haggins Adrianne N , Harney Deneil , Scott Sara , Silbergleit Robert 
Published: October 2017

Background: Exception from informed consent imposes community consultation and public disclosure requirements on clinical investigation in critically ill and injured patients. In 2011, the Food and Drug Administration instructed sponsors to submit publically disclosed information to the Food and Drug Administration Docket, but to date there has been no comprehensive analysis of available data. We summarized the community consultation and public disclosure practices of exception from informed consent trials published on the Food and Drug Administration Docket in order to better understand the breadth of common practices that exists among acute care clinical research. Methods: We performed quantitative and qualitative analysis of Docket FDA-1995-S-0036 from its initiation until June 2017 in order to summarize existing practices. We developed a 4-point scoring system to categorize public disclosure and community consultation based on inclusion of key components such as a detailed plan, schedule of events conducted, results, and materials uploaded. Results: The 177 docket submissions represented 34 trials. Material related to public disclosure accounted for 49% of pages, community consultation 45%, and 6% other. The median Docket Review Content Score for public disclosure was 3 (mean: 2.5, range: 0–4) and 2 (mean: 2.1, range: 0–4) for community consultation materials. Conclusion: The public information contained in the Docket varies broadly by trial and content. Additionally, as evidenced by the wide range of the Docket Review Content Score, submission guidelines are not followed uniformly. Given the apparent uncertainty about what should be submitted, and the need for best practice recommendations, it is valuable to categorize and summarize existing community consultation and public disclosure content.
Preventing disability in inflammatory bowel disease
Authors: Allen Patrick B. , Gower-Rousseau Corinne , Danese Silvio , Peyrin-Biroulet Laurent 
Published: October 2017

Disability is a common worldwide health challenge and it has been increasing over the past 3 decades. The treatment paradigm has changed dramatically in inflammatory bowel diseases (IBDs) from control of symptoms towards full control of disease (clinical and endoscopic remission) with the goal of preventing organ damage and disability. These aims are broadly similar to rheumatoid arthritis and multiple sclerosis. Since the 1990s, our attention has focused on quality of life in IBD, which is a subjective measure. However, as an objective end-point in clinical trials and population studies, measures of disability in IBD have been proposed. Disability is defined as ‘…any restriction or lack (resulting from an impairment) of ability to perform an activity in the manner or within the range considered normal for a human being.’ Recently, after 10 years of an international collaborative effort with the World Health Organization (WHO), a disability index was developed and validated. This index ideally would assist with the assessment of disease progression in IBD. In this review, we will provide the evidence to support the use of disability in IBD patients, including experience from rheumatoid arthritis and multiple sclerosis. New treatment strategies, and validation studies that have underpinned the interest and quantification of disability in IBD, will be discussed.
Sport-Related Concussions: Symptom Recurrence After Return to Exercise
Authors: O’Brien Michael J. , Howell David R. , Pepin Michael J. , Meehan William P. 
Published: October 2017

Background: Current guidelines dictate a gradual exercise progression after a concussion; however, it is unclear what proportion of athletes experience a recurrence of symptoms once they are symptom free at rest. Estimating the proportion of athletes and predictors of symptom recurrence would help shape return-to-play protocols. Purpose: To determine the proportion and associated risk factors of athletes who have a recurrence of concussion symptoms with exercise after being symptom free at rest. Study Design: Case-control study; Level of evidence, 3. Methods: Between October 1, 2009 and July 31, 2011, we studied patients from a sport concussion clinic located within a tertiary care regional children’s hospital. Patients were queried at every visit using a standardized questionnaire. Our main outcome variable was recurrence of symptoms with exercise after being symptom free at rest at some point in their recovery. Cofactors included age, sex, loss of consciousness with injury, prior concussion (diagnosed and undiagnosed), Post-Concussion Symptom Scale (PCSS) score, time until clinical presentation, and duration of symptoms. Results: Of the 217 patients included, 25 (12%) experienced a return of symptoms. Losing consciousness at the time of injury and a longer duration between injury and clinical presentation were associated with a decreased risk of symptoms recurring with exercise. Conversely, athletes who had sustained previously undiagnosed concussions and had suffered a longer duration of symptoms at rest were at an increased risk of symptom recurrence with exercise. Conclusion: Relatively few athletes who are symptom free at rest after a concussion will have a recurrence of symptoms when they resume exercise. The risk of symptoms recurring with exercise may be greater among those athletes who sustained previously undiagnosed concussions and had a longer period of symptoms at rest. The early identification of athletes who may be at risk of symptom recurrence will help mold treatment guidelines and exercise progression protocols.
A Biomechanical Comparison of Limited Open Versus Krackow Repair for Achilles Tendon Rupture
Authors: Dekker Robert G. , Qin Charles , Lawton Cort , Muriuki Muturi G. , Havey Robert M. , Alshouli Mohammed , Patwardhan Avinash G. , Kadakia Anish 
Published: October 2017

Background: Soft tissue complications after Achilles tendon repair has led to increased interest in less invasive techniques. Various limited open techniques have gained popularity as an alternative to open operative repair. The purpose of this study was to biomechanically compare an open Krackow and limited open repair for Achilles tendon rupture. We hypothesized that there would be no statistical difference in load to failure, work to failure, and initial linear stiffness. Methods: A simulated Achilles tendon rupture was created 4 cm proximal to its insertion in 18 fresh-frozen cadaveric below-knee lower limbs. Specimens were randomized to open or limited open PARS Achilles Jig System repair. Repairs were loaded to failure at a rate of 25.4 mm/s to reflect loading during normal ankle range of motion. Load to failure, work to failure, and initial linear stiffness were compared between the 2 repair types. Results: The average load to failure (353.8 ± 88.8 N vs 313.3 ± 99.9 N; P = .38) and work to failure (6.4 ± 2.3 J vs 6.3 ± 3.5 J; P = .904) were not statistically different for Krackow and PARS repair, respectively. Mean initial linear stiffness of the Krackow repair (17.8 ± 5.4 N/mm) was significantly greater than PARS repair (11.8 ± 2.5 N/mm) ( P = .011). Conclusion: No significant difference in repair strength was seen, but higher initial linear stiffness for Krackow repair suggests superior resistance to gap formation, which may occur during postoperative rehabilitation. With equal repair strength, but less soft tissue devitalization, the PARS may be a favorable option for patients with risk factors for soft tissue complications.
Safety and efficacy of long-term treatment of chronic/persistent ITP with eltrombopag: final results of the EXTEND study
Authors: Wong Raymond S. M. , Saleh Mansoor N. , Khelif Abderrahim , Salama Abdulgabar , Portella Maria Socorro O. , Burgess Paul , Bussel James B. 
Published: October 2017

Key Points
Median platelet counts increased to 50 × 109/L or more by week 2 in patients with ITP and were maintained for ≥2 years. Lower platelet counts, more previous therapies, and/or splenectomy resulted in good but somewhat lower responses to eltrombopag.
Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors
Authors: Meliani Amine , Boisgerault Florence , Fitzpatrick Zachary , Marmier Solenne , Leborgne Christian , Collaud Fanny , Simon Sola Marcelo , Charles Severine , Ronzitti Giuseppe , Vignaud Alban , van Wittenberghe Laetitia , Marolleau Beatrice , Jouen Fabienne , Tan Sisareuth , Boyer Olivier , Christophe Olivier , Brisson Alain R. , Maguire Casey A. , Mingozzi Federico 
Published: October 2017

Key Points
Enveloped AAV vectors are able to transduce the liver highly efficiently, driving superior correction of hemophilia B in mice. Enveloped AAVs are less susceptible to antibody-mediated neutralization, allowing for liver transduction in preimmunized animals.
Effect of alginate concentration on chondrogenesis of co-cultured human adipose-derived stem cells and nasal chondrocytes: a biological study
Authors: Ewa-Choy Y. W. , Pingguan-Murphy B. , Abdul-Ghani N. A. , Jahendran J. , Chua K. H. 
Published: October 2017

Abstract

Background
The three-dimensional (3D) system is one of the important factors to engineer a biocompatible and functional scaffold for the applications of cell-based therapies for cartilage repair. The 3D alginate hydrogels system has previously been shown to potentially promote chondrogenesis. The chondrocytic differentiation of co-cultured adipose-derived stem cells (ADSCs) and nasal chondrocytes (NCs) within alginate constructs are hypothesized to be influenced by concentration of alginate hydrogel. In this study, we evaluated the effects of alginate concentration on chondrogenic differentiation of ADSCs and NCs co-cultured in a biological approach.

Method
The co-cultured cells of 2:1 ADSCs-to-NCs ratio were encapsulated in alginate constructs in one of three concentrations (1.0%, 1.2% and 1.5%) and cultured under serum free conditions for 7 days. Cell viability, cell proliferation, immunohistochemical, gycosaminogylycans (GAG) synthesis, and gene expression were examined.

Results
Overall, the 1.2% alginate concentration group was relatively effective in chondrocytic differentiation in comparable to other groups. The cell morphology, cell viability, and cell proliferation revealed initial chondrogenic differentiation by the formation of cell clusters as well as the high permeability for exchange of solutes. The formation of newly synthesis cartilage-specific extracellular matrix in 1.2% group was demonstrated by positive immunohistochemical staining of collagen type II. The co-cultured cells in 1.2% group highly expressed COL II, ACP and SOX-9, compared to 1.0% and 1.5% groups, denote the retention of cartilaginous-specific phenotype by suppressing the undifferentiation stem cell markers of SOX-2 and OCT-4. The study showed 1.2% group was less likely to differentiate towards osteogenesis by downregulating hyperthrophy chondrocytic gene of COL X and osseous marker genes of OSC and OSP.

Conclusion
This study suggests that variations in the alginate concentration of co-cultured ADSCs and NCs influenced the chondrogenesis. The remarkable biological performance on chondrogenic differentiation in regulating the concentration of alginate 3D culture provides new insights into the cell cross-talk and demonstrates the effectiveness in regenerative therapies of cartilage defects in tissue engineering.
Oligochitosan-stabilized photoluminescent gold nanoconstructs for optical bioimaging
Authors: Yoo Donghyuck , Lee Dongwon 
Published: October 2017

Abstract

Background

Gold nanoclusters (AuNCs) are typically composed of several to tens of gold atoms which are stabilized with biomacromolecules such as bovine serum albumin (BSA). Au NCs fluoresces in the visible to near infrared region, in a size-dependent manner. AuNCs solutions have potential as fluorophore in a wide range of biomedical applications such as biodetection, biosensing and bioimaging in vitro and in vivo
.
However, their stability and harsh condition of preparation limit their biomedical application.

Methods

BSA stabilized AuNCs (BSA-AuNCs) were prepared by mixing HAuCl
4
solution with BSA solution for 24 h at 37°C under basic condition. BSA-AuNCs were then mixed with oliogochitosan (OCS) to generate BSA-Au-OCS nanocomplexes. The physicochemical and optical properties of BSA-Au-OCS nanocomplexes were studied using a fluorospectrometer. Their potential as a bioimaging agent in vivo and in vitro was evaluated using a fluorescent imaging instrument.

Results
BSA-stabilized AuNCs solutions were mixed with oligochitosan (OCS) to develop BSA-Au-OCS nanocomplexes of a mean diameter of ~250 nm. BSA-Au-OCS nanocomplexes could emit light at 620 nm and the complexation with OCS did not affect the photophysical properties of BSA-AuNCs. BSA-Au-OCS nanocomplexes showed less cytotoxicity than BSA-AuNCs and was readily taken up by cells. BSA-Au-OCS nanocomplexes showed strong fluorescence in tissues.

Conclusions
We developed stable BSA-Au-OCS nanocomplexes which fluoresce in the near infrared region. BSA-Au-OCS nanocomplexes exhibited significantly less cytotoxicity and strong fluorescence emission, suggesting potential for biomedical applications.