bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social nature of eating could explain missing link between food insecurity and childhood obesity
Authors: Mata Jutta , Dallacker Mattea , Hertwig Ralph 
Published: May 2017

AbstractWe suggest that social factors are key to explain the missing link between food insecurity and obesity in children. Parents and public institutions are children's nutritional gatekeepers. They protect children from food insecurity by trimming down their consumption or by institutional support. To gauge children's food insecurity, evaluations across the different nutritional gatekeepers need to be integrated.
“It's a bit more complicated than that”: A broader perspective on determinants of obesity
Authors: Mullan Barbara , Ntoumanis Nikos , Thøgersen-Ntoumani Cecilie , Lipp Ottmar V. 
Published: May 2017

AbstractThe insurance hypothesis does not address important factors known to contribute to obesity levels in all persons, not just adult women in the industrialized world. These include psychological determinants of eating behaviours, the decline in physical activity leading to a negative energy balance, the dense built environment, pervasive food marketing, and the increased availability of energy-dense, nutrient-poor food.
Using food insecurity in health prevention to promote consumer's embodied self-regulation
Authors: Petit Olivia , Spence Charles 
Published: May 2017

AbstractHealth messages designed to address obesity are typically focused on the long-term benefits of eating healthy food. However, according to the insurance hypothesis, obese people are food insecure, and this causes them to be overly concerned about short-term consumption. As such, it is necessary to rethink public health messaging and consider how to reduce short-term insecurity by eating healthy food.
Neither “Bad” nor “Dirty”: High-end Sex Work and Intimate Relationships in Urban China
Authors: Yuk-ha Tsang Eileen 
Published: May 2017

AbstractThe relationship between sex workers and their clients is generally characterized as being based entirely on the direct exchange of sexual favours for money. However, this received wisdom cannot account for a nation such as China which attaches significant value to “face,” social status and interpersonal dignity. This paper draws parallels with high-end sex workers elsewhere in Asia as well as globally. With a focus on the high-end sector, I examine how workers and their clients engage not only in pecuniary transactions but also in genuinely intimate and non-remunerative relationships. High-end sex workers make use of their earned economic capital to acquire cultural capital, and use online apps as marketing tools to target local elites and expats to forge longer-term intimate relationships. Male clients in more commercialized, post-industrial cities in China continue to seek diverse types of sexual experiences, with some clients seeking genuine intimacy. Furthermore, I explore how Chinese and foreign clients overcome social barriers to develop such relationships with sex workers. Building on this sociocultural perspective, this paper analyses ethnographically both sides of the female sex worker–client relationship in high-end karaoke lounges and bars in Dongguan, southern China.
Outbreak of diarrhoea among participants of a triathlon and a duathlon on 12 July 2015 in Utrecht, the Netherlands
Authors: PARKKALI S. , JOOSTEN R. , FANOY E. , PIJNACKER R. , VAN BEEK J. , BRANDWAGT D. , VAN PELT W. 
Published: May 2017

SUMMARYOn 12 July 2015, a triathlon competition with 900 participants took place in Utrecht, the Netherlands. An outbreak investigation was initiated after 56 participants reported health complaints. An online questionnaire was sent to 700 participants. Stool specimens from six participants and four water specimens were collected from the swimming location. A total of 239 participants completed the questionnaire (response rate: 34%), 73 (31%) of them met the case definition for acute gastrointestinal illness (AGI). A total of 67% of the respondents were male and the median age was 38 years. Almost half (42%) of swimmers reported health complaints. Consumption of energy drinks and ingesting ⩾3 mouthfuls of canal water were identified as risk factors for AGI among swimmers only (adjusted relative risks (aRR) 1·6; 95% confidence intervals (CI) 1·0–2·5 and aRR 2·6; 95% CI 1·5–4·8). The collected water specimens tested positive for norovirus genogroup I and rotavirus and stool specimens tested positive for norovirus genogroup II. Our findings indicate that the outbreak could have been caused by exposure to norovirus during swimming. Swimmers should get information about the health risks for making an informed choice about participating. For future events, the organisers decided to change the swimming location from a canal to a recreational lake.
Temporal dynamics of gene expression and histone marks at the Arabidopsis shoot meristem during flowering
Authors: You Yuan , Sawikowska Aneta , Neumann Manuela , Posé David , Capovilla Giovanna , Langenecker Tobias , Neher Richard A. , Krajewski Paweł , Schmid Markus 
Published: May 2017

AbstractPlants can produce organs throughout their entire life from pluripotent stem cells located at their growing tip, the shoot apical meristem (SAM). At the time of flowering, the SAM of Arabidopsis thaliana switches fate and starts producing flowers instead of leaves. Correct timing of flowering in part determines reproductive success, and is therefore under environmental and endogenous control. How epigenetic regulation contributes to the floral transition has eluded analysis so far, mostly because of the poor accessibility of the SAM. Here we report the temporal dynamics of the chromatin modifications H3K4me3 and H3K27me3 and their correlation with transcriptional changes at the SAM in response to photoperiod-induced flowering. Emphasizing the importance of tissue-specific epigenomic analyses we detect enrichments of chromatin states in the SAM that were not apparent in whole seedlings. Furthermore, our results suggest that regulation of translation might be involved in adjusting meristem function during the induction of flowering.
Hundreds of dual-stage antimalarial molecules discovered by a functional gametocyte screen
Authors: Miguel-Blanco Celia , Molina Irene , Bardera Ana I. , Díaz Beatriz , de las Heras Laura , Lozano Sonia , González Carolina , Rodrigues Janneth , Delves Michael J. , Ruecker Andrea , Colmenarejo Gonzalo , Viera Sara , Martínez-Martínez María S. , Fernández Esther , Baum Jake , Sinden Robert E. , Herreros Esperanza 
Published: May 2017

AbstractPlasmodium falciparum stage V gametocytes are responsible for parasite transmission, and drugs targeting this stage are needed to support malaria elimination. We here screen the Tres Cantos Antimalarial Set (TCAMS) using the previously developed P. falciparum female gametocyte activation assay (Pf FGAA), which assesses stage V female gametocyte viability and functionality using Pfs25 expression. We identify over 400 compounds with activities <2 μM, chemically classified into 57 clusters and 33 singletons. Up to 68% of the hits are chemotypes described for the first time as late-stage gametocyte-targeting molecules. In addition, the biological profile of 90 compounds representing the chemical diversity is assessed. We confirm in vitro transmission-blocking activity of four of the six selected molecules belonging to three distinct scaffold clusters. Overall, this TCAMS gametocyte screen provides 276 promising antimalarial molecules with dual asexual/sexual activity, representing starting points for target identification and candidate selection.
YAP/TAZ link cell mechanics to Notch signalling to control epidermal stem cell fate
Authors: Totaro Antonio , Castellan Martina , Battilana Giusy , Zanconato Francesca , Azzolin Luca , Giulitti Stefano , Cordenonsi Michelangelo , Piccolo Stefano 
Published: May 2017

AbstractHow the behaviour of somatic stem cells (SCs) is influenced by mechanical signals remains a black-box in cell biology. Here we show that YAP/TAZ regulation by cell shape and rigidity of the extracellular matrix (ECM) dictates a pivotal SC decision: to remain undifferentiated and grow, or to activate a terminal differentiation programme. Notably, mechano-activation of YAP/TAZ promotes epidermal stemness by inhibition of Notch signalling, a key factor for epidermal differentiation. Conversely, YAP/TAZ inhibition by low mechanical forces induces Notch signalling and loss of SC traits. As such, mechano-dependent regulation of YAP/TAZ reflects into mechano-dependent regulation of Notch signalling. Mechanistically, at least in part, this is mediated by YAP/TAZ binding to distant enhancers activating the expression of Delta-like ligands, serving as ‘in cis’ inhibitors of Notch. Thus YAP/TAZ mechanotransduction integrates with cell–cell communication pathways for fine-grained orchestration of SC decisions.
Giant tunnelling electroresistance in metal/ferroelectric/semiconductor tunnel junctions by engineering the Schottky barrier
Authors: Xi Zhongnan , Ruan Jieji , Li Chen , Zheng Chunyan , Wen Zheng , Dai Jiyan , Li Aidong , Wu Di 
Published: May 2017

AbstractRecently, ferroelectric tunnel junctions have attracted much attention due to their potential applications in non-destructive readout non-volatile memories. Using a semiconductor electrode has been proven effective to enhance the tunnelling electroresistance in ferroelectric tunnel junctions. Here we report a systematic investigation on electroresistance of Pt/BaTiO3/Nb:SrTiO3 metal/ferroelectric/semiconductor tunnel junctions by engineering the Schottky barrier on Nb:SrTiO3 surface via varying BaTiO3 thickness and Nb doping concentration. The optimum ON/OFF ratio as great as 6.0 × 106, comparable to that of commercial Flash memories, is achieved in a device with 0.1 wt% Nb concentration and a 4-unit-cell-thick BaTiO3 barrier. With this thinnest BaTiO3 barrier, which shows a negligible resistance to the tunnelling current but is still ferroelectric, the device is reduced to a polarization-modulated metal/semiconductor Schottky junction that exhibits a more efficient control on the tunnelling resistance to produce the giant electroresistance observed. These results may facilitate the design of high performance non-volatile resistive memories.
Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast
Authors: Rincon Sergio A. , Lamson Adam , Blackwell Robert , Syrovatkina Viktoriya , Fraisier Vincent , Paoletti Anne , Betterton Meredith D. , Tran Phong T. 
Published: May 2017

AbstractBipolar spindle assembly requires a balance of forces where kinesin-5 produces outward pushing forces to antagonize the inward pulling forces from kinesin-14 or dynein. Accordingly, Kinesin-5 inactivation results in force imbalance leading to monopolar spindle and chromosome segregation failure. In fission yeast, force balance is restored when both kinesin-5 Cut7 and kinesin-14 Pkl1 are deleted, restoring spindle bipolarity. Here we show that the cut7Δpkl1Δ spindle is fully competent for chromosome segregation independently of motor activity, except for kinesin-6 Klp9, which is required for anaphase spindle elongation. We demonstrate that cut7Δpkl1Δ spindle bipolarity requires the microtubule antiparallel bundler PRC1/Ase1 to recruit CLASP/Cls1 to stabilize microtubules. Brownian dynamics-kinetic Monte Carlo simulations show that Ase1 and Cls1 activity are sufficient for initial bipolar spindle formation. We conclude that pushing forces generated by microtubule polymerization are sufficient to promote spindle pole separation and the assembly of bipolar spindle in the absence of molecular motors.
Experimental quantum compressed sensing for a seven-qubit system
Authors: Riofrío C. A. , Gross D. , Flammia S. T. , Monz T. , Nigg D. , Blatt R. , Eisert J. 
Published: May 2017

AbstractWell-controlled quantum devices with their increasing system size face a new roadblock hindering further development of quantum technologies. The effort of quantum tomography—the reconstruction of states and processes of a quantum device—scales unfavourably: state-of-the-art systems can no longer be characterized. Quantum compressed sensing mitigates this problem by reconstructing states from incomplete data. Here we present an experimental implementation of compressed tomography of a seven-qubit system—a topological colour code prepared in a trapped ion architecture. We are in the highly incomplete—127 Pauli basis measurement settings—and highly noisy—100 repetitions each—regime. Originally, compressed sensing was advocated for states with few non-zero eigenvalues. We argue that low-rank estimates are appropriate in general since statistical noise enables reliable reconstruction of only the leading eigenvectors. The remaining eigenvectors behave consistently with a random-matrix model that carries no information about the true state.
Thermophoretic migration of vesicles depends on mean temperature and head group chemistry
Authors: Talbot Emma L. , Kotar Jurij , Parolini Lucia , Di Michele Lorenzo , Cicuta Pietro 
Published: May 2017

AbstractA number of colloidal systems, including polymers, proteins, micelles and hard spheres, have been studied in thermal gradients to observe and characterize their driven motion. Here we show experimentally the thermophoretic behaviour of unilamellar lipid vesicles, finding that mobility depends on the mean local temperature of the suspension and on the structure of the exposed polar lipid head groups. By tuning the temperature, vesicles can be directed towards hot or cold, forming a highly concentrated region. Binary mixtures of vesicles composed of different lipids can be segregated using thermophoresis, according to their head group. Our results demonstrate that thermophoresis enables robust and chemically specific directed motion of liposomes, which can be exploited in driven processes.
Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand
Authors: Zhang Xianjun , Zhao Fei , Wu Yiran , Yang Jun , Han Gye Won , Zhao Suwen , Ishchenko Andrii , Ye Lintao , Lin Xi , Ding Kang , Dharmarajan Venkatasubramanian , Griffin Patrick R. , Gati Cornelius , Nelson Garrett , Hunter Mark S. , Hanson Michael A. , Cherezov Vadim , Stevens Raymond C. , Tan Wenfu , Tao Houchao , Xu Fei 
Published: May 2017

AbstractThe Smoothened receptor (SMO) belongs to the Class Frizzled of the G protein-coupled receptor (GPCR) superfamily, constituting a key component of the Hedgehog signalling pathway. Here we report the crystal structure of the multi-domain human SMO, bound and stabilized by a designed tool ligand TC114, using an X-ray free-electron laser source at 2.9 Å. The structure reveals a precise arrangement of three distinct domains: a seven-transmembrane helices domain (TMD), a hinge domain (HD) and an intact extracellular cysteine-rich domain (CRD). This architecture enables allosteric interactions between the domains that are important for ligand recognition and receptor activation. By combining the structural data, molecular dynamics simulation, and hydrogen-deuterium-exchange analysis, we demonstrate that transmembrane helix VI, extracellular loop 3 and the HD play a central role in transmitting the signal employing a unique GPCR activation mechanism, distinct from other multi-domain GPCRs.
Decoding material-specific memory reprocessing during sleep in humans
Authors: Schönauer M. , Alizadeh S. , Jamalabadi H. , Abraham A. , Pawlizki A. , Gais S. 
Published: May 2017

AbstractNeuronal learning activity is reactivated during sleep but the dynamics of this reactivation in humans are still poorly understood. Here we use multivariate pattern classification to decode electrical brain activity during sleep and determine what type of images participants had viewed in a preceding learning session. We find significant patterns of learning-related processing during rapid eye movement (REM) and non-REM (NREM) sleep, which are generalizable across subjects. This processing occurs in a cyclic fashion during time windows congruous to critical periods of synaptic plasticity. Its spatial distribution over the scalp and relevant frequencies differ between NREM and REM sleep. Moreover, only the strength of reprocessing in slow-wave sleep influenced later memory performance, speaking for at least two distinct underlying mechanisms between these states. We thus show that memory reprocessing occurs in both NREM and REM sleep in humans and that it pertains to different aspects of the consolidation process.
Electricity from methane by reversing methanogenesis
Authors: McAnulty Michael J. , G. Poosarla Venkata , Kim Kyoung-Yeol , Jasso-Chávez Ricardo , Logan Bruce E. , Wood Thomas K. 
Published: May 2017

AbstractGiven our vast methane reserves and the difficulty in transporting methane without substantial leaks, the conversion of methane directly into electricity would be beneficial. Microbial fuel cells harness electrical power from a wide variety of substrates through biological means; however, the greenhouse gas methane has not been used with much success previously as a substrate in microbial fuel cells to generate electrical current. Here we construct a synthetic consortium consisting of: (i) an engineered archaeal strain to produce methyl-coenzyme M reductase from unculturable anaerobic methanotrophs for capturing methane and secreting acetate; (ii) micro-organisms from methane-acclimated sludge (including Paracoccus denitrificans) to facilitate electron transfer by providing electron shuttles (confirmed by replacing the sludge with humic acids), and (iii) Geobacter sulfurreducens to produce electrons from acetate, to create a microbial fuel cell that converts methane directly into significant electrical current. Notably, this methane microbial fuel cell operates at high Coulombic efficiency.
Neuromorphic device architectures with global connectivity through electrolyte gating
Authors: Gkoupidenis Paschalis , Koutsouras Dimitrios A. , Malliaras George G. 
Published: May 2017

AbstractInformation processing in the brain takes place in a network of neurons that are connected with each other by an immense number of synapses. At the same time, neurons are immersed in a common electrochemical environment, and global parameters such as concentrations of various hormones regulate the overall network function. This computational paradigm of global regulation, also known as homeoplasticity, has important implications in the overall behaviour of large neural ensembles and is barely addressed in neuromorphic device architectures. Here, we demonstrate the global control of an array of organic devices based on poly(3,4ethylenedioxythiophene):poly(styrene sulf) that are immersed in an electrolyte, a behaviour that resembles homeoplasticity phenomena of the neural environment. We use this effect to produce behaviour that is reminiscent of the coupling between local activity and global oscillations in the biological neural networks. We further show that the electrolyte establishes complex connections between individual devices, and leverage these connections to implement coincidence detection. These results demonstrate that electrolyte gating offers significant advantages for the realization of networks of neuromorphic devices of higher complexity and with minimal hardwired connectivity.
An integrated model for detecting significant chromatin interactions from high-resolution Hi-C data
Authors: Carty Mark , Zamparo Lee , Sahin Merve , González Alvaro , Pelossof Raphael , Elemento Olivier , Leslie Christina S. 
Published: May 2017

AbstractHere we present HiC-DC, a principled method to estimate the statistical significance (P values) of chromatin interactions from Hi-C experiments. HiC-DC uses hurdle negative binomial regression account for systematic sources of variation in Hi-C read counts—for example, distance-dependent random polymer ligation and GC content and mappability bias—and model zero inflation and overdispersion. Applied to high-resolution Hi-C data in a lymphoblastoid cell line, HiC-DC detects significant interactions at the sub-topologically associating domain level, identifying potential structural and regulatory interactions supported by CTCF binding sites, DNase accessibility, and/or active histone marks. CTCF-associated interactions are most strongly enriched in the middle genomic distance range (∼700 kb–1.5 Mb), while interactions involving actively marked DNase accessible elements are enriched both at short (<500 kb) and longer (>1.5 Mb) genomic distances. There is a striking enrichment of longer-range interactions connecting replication-dependent histone genes on chromosome 6, potentially representing the chromatin architecture at the histone locus body.
The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
Authors: Tirumalai Madhan R. , Karouia Fathi , Tran Quyen , Stepanov Victor G. , Bruce Rebekah J. , Ott C. Mark , Pierson Duane L. , Fox George E. 
Published: May 2017

AbstractMicroorganisms impact spaceflight in a variety of ways. They play a positive role in biological systems, such as waste water treatment but can be problematic through buildups of biofilms that can affect advanced life support. Of special concern is the possibility that during extended missions, the microgravity environment will provide positive selection for undesirable genomic changes. Such changes could affect microbial antibiotic sensitivity and possibly pathogenicity. To evaluate this possibility, Escherichia coli (lac plus) cells were grown for over 1000 generations on Luria Broth medium under low-shear modeled microgravity conditions in a high aspect rotating vessel. This is the first study of its kind to grow bacteria for multiple generations over an extended period under low-shear modeled microgravity. Comparisons were made to a non-adaptive control strain using growth competitions. After 1000 generations, the final low-shear modeled microgravity-adapted strain readily outcompeted the unadapted lac minus strain. A portion of this advantage was maintained when the low-shear modeled microgravity strain was first grown in a shake flask environment for 10, 20, or 30 generations of growth. Genomic sequencing of the 1000 generation strain revealed 16 mutations. Of the five changes affecting codons, none were neutral. It is not clear how significant these mutations are as individual changes or as a group. It is concluded that part of the long-term adaptation to low-shear modeled microgravity is likely genomic. The strain was monitored for acquisition of antibiotic resistance by VITEK analysis throughout the adaptation period. Despite the evidence of genomic adaptation, resistance to a variety of antibiotics was never observed.