bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of drought stress tolerance in wild species germplasm of rice based on leaf and root morphology
Authors: Neelam Kumari , Sahi Gurpreet K. , Kumar Kishor , Singh Kuldeep 
Published: October 2017

AbstractDrought is the major abiotic constraint to the rice production in the rain-fed areas across Asia and sub-Saharan Africa. Wild species of Oryza offer a wide spectrum of adaptive traits and can serve as potential donors of biotic and abiotic stress tolerance. At the Punjab Agricultural University, we are maintaining an active collection of 1630 accessions of wild species germplasm (AA, CC, BBCC and CCDD) of rice. These accessions were screened to assess genetic variation for drought tolerance under field conditions. Severe water stress was imposed at the late vegetative stage by withholding water initially for 25 d and then extended further to 35 d during kharif season in the years 2013–14 and 2015–16. The tolerance score for drought stress was based on the extent of leaf rolling and leaf drying. Based on the 2 years’ data, seven accessions from Oryza rufipogon, four from Oryza longistaminata and one each from Oryza officinalis and Oryza latifolia were found tolerant to drought stress. These selected accessions were further phenotype for root morphology. The average root length among the selected accessions ranges between 36 and 80 cm and the number of primary roots vary from 30 to 87 cm. The O. rufipogon accession IRGC 106433, O. longistaminata accession IRGC 92656A, O. officinalis accession IRGC 101152 and O. latifolia accession IRGC 80769 showed approximately 2–2.5 times longer root length and number than the indica rice elite cultivar PR121. The results indicated potentiality of selected wild species germplasm for conferring drought tolerance to the elite cultivars.
Loneliness, health and mortality
Authors: Henriksen J. , Larsen E. R. , Mattisson C. , Andersson N. W. 
Published: October 2017

Aims.Literature suggests an association between loneliness and mortality for both males and females. Yet, the linkage of loneliness to mortality is not thoroughly examined, and need to be replicated with a long follow-up time. This study assessed the association between loneliness and mortality, including associations to gender, in 1363 adult swedes.Methods.This community-based prospective cohort study from the Swedish Lundby Study included 1363 individuals of whom 296 individuals (21.7%) were identified as lonely with use of semi-structured interviews in 1997. The cohort was followed until 2011 and survival analyses were used to estimate the relative risk of death.Results.Death occurred with an incidence rate of 2.63 per 100 person-years and 2.09 per 100 person-years for lonely and non-lonely individuals, respectively. In crude analysis, loneliness was associated with a significant increased mortality risk of 27% compared with non-lonely individuals [hazard ratio (HR) 1.27; 95% CI 1.01–1.60]. Unadjusted, lonely females had a significant increased risk (HR 1.76; 95% CI 1.31–2.34) and adjusted insignificant increased mortality risk of 27% (HR 1.27; 95% CI 0.92–1.74), compared with non-lonely females. Lonely males were found to have an adjusted significant decreased risk of mortality (HR 0.50; 95% CI 0.32–0.80), compared with non-lonely males.Conclusions.Findings suggest an association between loneliness and increased risk of mortality and that gender differences may exist, which have not been previously reported. If replicated, our results indicate that loneliness may have differential physical implications in some subgroups. Future studies are needed to further investigate the influence of gender on the relationship.
Gender Differences in the Influence of Parenting on Youth Antisocial Behavior through Deviant Peers
Authors: Cutrín Olalla , Gómez-Fraguela José Antonio , Sobral Jorge 
Published: October 2017

AbstractThe aim of this study was to assess gender differences in direct and indirect effects of parental knowledge, family support, family conflict, and deviant peers on violent and nonviolent antisocial behavior among youngsters. The total sample was composed of 584 young people, 274 males and 310 females, aged 14 to 20 from High Schools of Galicia (NW Spain). The variables were assessed with different scales of the protocol Valoración del Riesgo en Adolescentes Infractores [Juvenile Offender’s Risk Assessment]. Several structural equation models were conducted to clarify the relationships between these variables for males and females. The results showed a better fit for the mediated model. Significant direct effects were found for parental knowledge (β = –.35, p < .01, males; β = –.16, p < .05, females) and parental support (β = .26, p < .05, males) on nonviolent behavior. Significant direct effects were also found for parental knowledge (β = –.36, p < .05, males; β = –.42, p < .05, females) and parental support (β = .32, p < .05, males; β = .24, p < .05, females) on violent behavior. Not significant direct effects were found for family conflict. Moreover, significant indirect effects through deviant peers were found for knowledge (β = –.23, p < .01, males; β = –.21, p < .01, females), support (β = .20, p < .05, males; β = .21, p < .05, females), and conflict (β = .28, p < .01, males; β = .26, p < .05, females) on nonviolent behavior, as well as for knowledge (β = –.20, p < .01, males; β = –.10, p < .01, females), support (β = .18, p < .01, males; β = .10, p < .01, females), and conflict (β = .24, p < .05, males; β = .12, p < .01, females) on violent behavior. Thus, significant gender differences were found, specifically in the direct effects of family support on nonviolent antisocial behavior. The implications of these results for prevention of antisocial behaviors in youth based on gender differences are discussed.
Endovascular treatment of penetrating arterial trauma with stent grafts
Authors: Biagioni Rodrigo Bruno , Burihan Marcelo Calil , Nasser Felipe , Biagioni Luisa Ciucci , Ingrund José Carlos 
Published: October 2017

Abstract. Background: The endovascular management of arterial injuries has resulted in reduced operating time, blood loss, hospital mortality, lower incidence of sepsis, and decrease in mortality rates. For penetrating trauma, however, the benefits of endovascular therapy are questionable. Patients and methods: Data were obtained by retrospective analysis of electronic medical records. All patients with vascular trauma seeking care at our institution from January 2010 to December 2015 were reviewed. A total of 223 vascular trauma patients were enrolled. Of these, 18 patients (8 %) were treated with endovascular techniques. The data related to clinical presentation, patient characteristics, technical aspects of the treatment, and follow-up were analysed. Results: The mean patient age was 35.4 ± 17.8 years, 94 % were male. The mean injury severity score was 10.4 ± 2.5. The most commonly observed trauma mechanism was a gunshot in 10 cases (55 %), followed by lesions provoked by arterial catheter misplacement in five cases (27 %), and stab wounds in three cases (16.6 %). The main injury site was the subclavian artery, accounting for eight cases (44 %), followed by the superficial femoral artery and the tibiofibular trunk in two cases, respectively (18 %). The anterior tibial, fibular artery, axillary, common carotid, superior mesenteric, and profunda femoris were each affected once. Arteriovenous fistula was detected in nine cases (50 %), pseudoaneurysms in nine cases (50 %), and short occlusion in two cases (11 %). The mean follow-up duration was 753 days. The primary patency rate was 92.3 and 61.5 % after one and two years, respectively. The survival rate was 94.4 % after one and two years. Infection of the stents or limb amputations were not identified at follow-up. Conclusions: The endovascular treatment of penetrating arterial injuries with covered stents is feasible. However, the criteria used to choose the best method must be individualized. Keywords: Trauma, endovascular, stent graft, gunshot, stab wound
Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells
Authors: Toulany Mahmoud , Maier Julia , Iida Mari , Rebholz Simone , Holler Marina , Grottke Astrid , Jüker Manfred , Wheeler Deric L , Rothbauer Ulrich , Rodemann H Peter 
Published: October 2017

AbstractAkt1 through the C-terminal domain interacts with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and stimulates the repair of DNA double-strand breaks (DSBs) in K-RAS-mutated (K-RASmut) cells. We investigated the interactions of distinct domain(s) of DNA-PKcs in binding to full-length Akt1. Similarly, we analyzed potential interactions of DNA-PKcs with Akt2 and Akt3. Finally the effect of Akt isoforms in cell proliferation and tumor growth was tested. We demonstrated that Akt1 preferentially binds to the N-terminal domain of DNA-PKcs using pull-down studies with distinct eGFP-tagged DNA-PKcs fragments that were expressed by plasmids in combination with mCherry-tagged full-length Akt isoforms. These binding studies also indicated an interaction with the intermediate and C-terminal domains of DNA-PKcs. In contrast, Akt3 interacted with all four DNA-PKcs fragments without a marked preference for any specific domain. Notably, we could not see binding of Akt2 to any of the tested DNA-PKcs fragments. In subsequent studies, we demonstrated that Akt inhibition interferes with binding of Akt1 to the N-terminal domain of DNA-PKcs. This indicated a correlation between Akt1 activity and the Akt1/DNA-PKcs complex formation. Finally, knockdown studies revealed that the depletion of endogenous Akt1 and Akt3, but not Akt2, inhibit clonogenic activity and repair of ionizing radiation (IR)-induced DNA DSBs, leading to radiosensitization. Furthermore, in a xenograft study the expression of shAkt1 or shAkt3, but not shAkt2 in K-RASmut breast cancer cell line MDA-MB-231 showed major tumor growth delay. Together, these data indicate that Akt1 and Akt3, but not Akt2, physically interact with DNA-PKcs, thus stimulating the repair of DSBs and therefore protecting K-RASmut cells against IR. Likewise, interaction of Akt isoforms with DNA-PKcs could be crucial for their role in regulating tumor growth.
Ultra-low and ultra-broad-band nonlinear acoustic metamaterials
Authors: Fang Xin , Wen Jihong , Bonello Bernard , Yin Jianfei , Yu Dianlong 
Published: October 2017

AbstractLinear acoustic metamaterials (LAMs) are widely used to manipulate sound; however, it is challenging to obtain bandgaps with a generalized width (ratio of the bandgap width to its start frequency) >1 through linear mechanisms. Here we adopt both theoretical and experimental approaches to describe the nonlinear chaotic mechanism in both one-dimensional (1D) and two-dimensional (2D) nonlinear acoustic metamaterials (NAMs). This mechanism enables NAMs to reduce wave transmissions by as much as 20–40 dB in an ultra-low and ultra-broad band that consists of bandgaps and chaotic bands. With subwavelength cells, the generalized width reaches 21 in a 1D NAM and it goes up to 39 in a 2D NAM, which overcomes the bandwidth limit for wave suppression in current LAMs. This work enables further progress in elucidating the dynamics of NAMs and opens new avenues in double-ultra acoustic manipulation.
Sensory coding accuracy and perceptual performance are improved during the desynchronized cortical state
Authors: Beaman Charles B. , Eagleman Sarah L. , Dragoi Valentin 
Published: October 2017

AbstractCortical activity changes continuously during the course of the day. At a global scale, population activity varies between the ‘synchronized’ state during sleep and ‘desynchronized’ state during waking. However, whether local fluctuations in population synchrony during wakefulness modulate the accuracy of sensory encoding and behavioral performance is poorly understood. Here, we show that populations of cells in monkey visual cortex exhibit rapid fluctuations in synchrony ranging from desynchronized responses, indicative of high alertness, to highly synchronized responses. These fluctuations are local and control the trial variability in population coding accuracy and behavioral performance in a discrimination task. When local population activity is desynchronized, the correlated variability between neurons is reduced, and network and behavioral performance are enhanced. These findings demonstrate that the structure of variability in local cortical populations is not noise but rather controls how sensory information is optimally integrated with ongoing processes to guide network coding and behavior.
High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
Authors: Steube Arndt , Schenk Tino , Tretyakov Alexander , Saluz Hans Peter 
Published: October 2017

AbstractGenome-wide mapping of transcription factor binding is generally performed by chemical protein–DNA crosslinking, followed by chromatin immunoprecipitation and deep sequencing (ChIP-seq). Here we present the ChIP-seq technique based on photochemical crosslinking of protein–DNA interactions by high-intensity ultraviolet (UV) laser irradiation in living mammalian cells (UV-ChIP-seq). UV laser irradiation induces an efficient and instant formation of covalent “zero-length” crosslinks exclusively between nucleic acids and proteins that are in immediate contact, thus resulting in a “snapshot” of direct protein–DNA interactions in their natural environment. Here we show that UV-ChIP-seq, applied for genome-wide profiling of the sequence-specific transcriptional repressor B-cell lymphoma 6 (BCL6) in human diffuse large B-cell lymphoma (DLBCL) cells, produces sensitive and precise protein–DNA binding profiles, highly enriched with canonical BCL6 DNA sequence motifs. Using this technique, we also found numerous previously undetectable direct BCL6 binding sites, particularly in condensed, inaccessible areas of chromatin.
The structural basis of proton driven zinc transport by ZntB
Authors: Gati Cornelius , Stetsenko Artem , Slotboom Dirk J. , Scheres Sjors H. W. , Guskov Albert 
Published: October 2017

AbstractZinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic, therefore dedicated import, export and storage proteins for tight regulation of the zinc concentration have evolved. In Enterobacteriaceae, several membrane transporters are involved in zinc homeostasis and linked to virulence. ZntB has been proposed to play a role in the export of zinc, but the transport mechanism of ZntB is poorly understood and based only on experimental characterization of its distant homologue CorA magnesium channel. Here, we report the cryo-electron microscopy structure of full-length ZntB from Escherichia coli together with the results of isothermal titration calorimetry, and radio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes. Our results show that ZntB mediates Zn2+ uptake, stimulated by a pH gradient across the membrane, using a transport mechanism that does not resemble the one proposed for homologous CorA channels.
Dense fluidized granular media in microgravity
Authors: Born Philip , Schmitz Johannes , Sperl Matthias 
Published: October 2017

AbstractHandling and transport of granular media are inevitably governed by the settling of particles. Settling into a dense state is one of the defining characteristics of granular media, among dissipation and absence of thermal agitation. Hence, settling complicates the adaptation of microscopic theories from atomic, molecular, or colloidal media to granular media. It is desirable to provide experiments in which selectively one of the granular characteristics is tuned to test suitable adaptation of a theory. Here we show that gas fluidization of granular media in microgravity is a suitable approach to achieve steady states closer to thermally agitated systems free of settling. We use diffusing-wave spectroscopy to compare the spatial homogeneity and the microscopic dynamics of gas-fluidized granular media on the ground and in drop tower flights with increasing packing densities up to full arrest. The gas fluidization on the ground leads to inhomogeneous states as known from fluidized beds, and partial arrest occurs at packing fractions lower than the full arrested packing. The granular medium in microgravity in contrast attains a homogeneous state with complete mobilization even close to full arrest. Fluidized granular media thus can be studied in microgravity with dynamics and packing fractions not achievable on the ground.
Developing a platform system for gene delivery: amplifying virus-like particles (AVLP) as an influenza vaccine
Authors: Wei Huiling , Chen Zhenhai , Elson Andrew , Li Zhuo , Abraham Mathew , Phan Shannon , Kristhnamurthy Sateesh , McCray Paul B. , Andrews Seth , Stice Steve , Sakamoto Kaori , Jones Cheryl , Tompkins S. Mark , He Biao 
Published: October 2017

AbstractDelivery of a gene of interest to target cells is highly desirable for translational medicine, such as gene therapy, regenerative medicine, vaccine development, and studies of gene function. Parainfluenza virus 5 (PIV5), a paramyxovirus with a negative-sense RNA genome, normally infects cells without causing obvious cytopathic effect, and it can infect many cell types. To exploit these features of PIV5, we established a system generating self-amplifying, virus-like particles (AVLP). Using enhanced green fluorescent protein (EGFP) as a reporter, AVLP encoding EGFP (AVLP–EGFP) successfully delivered and expressed the EGFP gene in primary human cells, including stem cells, airway epithelial cells, monocytes, and T cells. To demonstrate the application of this system for vaccine development, we generated AVLPs to express the HA and M1 antigens from the influenza A virus strain H5N1 (AVLP–H5 and AVLP–M1H5). Immunization of mice with AVLP–H5 and AVLP–M1H5 generated robust antibody and cellular immune responses. Vaccination with a single dose of AVLP–H5 and M1H5 completely protected mice against lethal H5N1 challenge, suggesting that the AVLP-based system is a promising platform for delivery of desirable genes.
Single Crystal Growth and Spin Polarization Measurements of Diluted Magnetic Semiconductor (BaK)(ZnMn)2As2
Authors: Zhao G. Q. , Lin C. J. , Deng Z. , Gu G. X. , Yu S. , Wang X. C. , Gong Z. Z. , Uemura Yasutomo J. , Li Y. Q. , Jin C. Q. 
Published: October 2017

AbstractRecently a new diluted magnetic semiconductor, (Ba,K)(Zn,Mn)2As2 (BZA), with high Curie temperature was discovered, showing an independent spin and charge-doping mechanism. This makes BZA a promising material for spintronics devices. We report the successful growth of a BZA single crystal for the first time in this study. An Andreev reflection junction, which can be used to evaluate spin polarization, was fabricated based on the BZA single crystal. A 66% spin polarization of the BZA single crystal was obtained by Andreev reflection spectroscopy analysis.
Transcriptional responses of wheat and the cereal cyst nematode Heterodera avenae during their early contact stage
Authors: Chen Changlong , Cui Lei , Chen Yongpan , Zhang Hongjun , Liu Pei , Wu Peipei , Qiu Dan , Zou Jingwei , Yang Dan , Yang Li , Liu Hongwei , Zhou Yang , Li Hongjie 
Published: October 2017

AbstractCereal cyst nematode (Heterodera avenae) is attracted to and aggregated around wheat roots to initiate infection, but this interaction between wheat and the nematode is not fully understood. The transcriptional responses of both wheat and H. avenae were examined during their early contact stage by mRNA sequencing analysis; certain numbers of the differentially expressed genes (DEGs) were validated using quantitative real-time PCR. The immobile host wheat root only had 93 DEGs (27 up-regulated and 66 down-regulated), while the mobile plant parasitic nematode reacted much more actively with 879 DEGs (867 up-regulated and 12 down-regulated). Among them, a number of wheat DEGs (mostly down-regulated) were involved in biotic stress pathways, while several putative effector genes were up-regulated in the nematode DEGs. One putative chitinase-like effector gene of H. avenae was able to suppress BAX-triggered programmed cell death in Nicotiana benthamiana. Results of these experiments demonstrated that nematode responded more actively than wheat during the contact stage of parasitism. The parasite’s responses mainly involved up-regulation of genes including at least one anti-plant-defence effector gene, whereas the host responses mainly involved down-regulation of certain defence-related genes.
Translational utility of a hierarchical classification strategy in biomolecular data analytics
Authors: Galea Dieter , Inglese Paolo , Cammack Lidia , Strittmatter Nicole , Rebec Monica , Mirnezami Reza , Laponogov Ivan , Kinross James , Nicholson Jeremy , Takats Zoltan , Veselkov Kirill A. 
Published: October 2017

AbstractHierarchical classification (HC) stratifies and classifies data from broad classes into more specific classes. Unlike commonly used data classification strategies, this enables the probabilistic prediction of unknown classes at different levels, minimizing the burden of incomplete databases. Despite these advantages, its translational application in biomedical sciences has been limited. We describe and demonstrate the implementation of a HC approach for “omics-driven” classification of 15 bacterial species at various taxonomic levels achieving 90–100% accuracy, and 9 cancer types into morphological types and 35 subtypes with 99% and 76% accuracy, respectively. Unknown bacterial species were probabilistically assigned with 100% accuracy to their respective genus or family using mass spectra (n = 284). Cancer types were predicted by mRNA data (n = 1960) for most subtypes with 95–100% accuracy. This has high relevance in clinical practice where complete datasets are difficult to compile with the continuous evolution of diseases and emergence of new strains, yet prediction of unknown classes, such as bacterial species, at upper hierarchy levels may be sufficient to initiate antimicrobial therapy. The algorithms presented here can be directly translated into clinical-use with any quantitative data, and have broad application potential, from unlabeled sample identification, to hierarchical feature selection, and discovery of new taxonomic variants.
Freeform micropatterning of living cells into cell culture medium using direct inkjet printing
Authors: Park Ju An , Yoon Sejeong , Kwon Jimin , Now Hesung , Kim Young Kwon , Kim Woo-Jong , Yoo Joo-Yeon , Jung Sungjune 
Published: October 2017

AbstractMicrofabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is still a challenge when co-culturing multiple types of cells on the same substrate. For the first time, we present a drop-on-demand inkjet printing method to directly pattern living cells into a cell-friendly liquid environment. High-resolution control of cell location is achieved by precisely optimizing printing parameters with high-speed imaging of cell jetting and impacting behaviors. We demonstrated the capabilities of the direct cell printing method by co-printing different cells into various designs, including complex gradient arrangements. Finally, we applied this technique to investigate the influence of the heterogeneity and geometry of the cell population on the infectivity of seasonal H1N1 influenza virus (PR8) by generating A549 and HeLa cells printed in checkboard patterns of different sizes in a medium-filled culture dish. Direct inkjet cell patterning can be a powerful and versatile tool for both fundamental biology and applied biotechnology.