bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological regulation of oral saliva ion composition and flow rate are not coupled in healthy humans—Partial revision of our current knowledge required
Authors: Schwerdt Gerald , Schulz Marie-Christin , Kopf Michael , Mildenberger Sigrid , Reime Sarah , Gekle Michael 
Published: October 2024

AbstractAppropriate composition of oral saliva is essential for a healthy milieu that protects mucosa and teeth. Only few studies, with small sample numbers, investigated physiological saliva ion composition in humans. We determined saliva ion composition in a sufficiently large cohort of healthy adults and analyzed the effect of physiological stimulation. We collected saliva from 102 adults under non-stimulated and physiologically stimulated conditions (chewing). Individual flow rates, pH, osmolality, Na+, K+, Cl−, and HCO3− concentrations under both conditions as well as the individual changes due to stimulation (Δvalues) were determined. Non-stimulated saliva was hypoosmolal and acidic. Na+, Cl−, and HCO3− concentrations remained well below physiological plasma values, whereas K+ concentrations exceeded plasma values more than twofold. Stimulation resulted in a doubling of flow rates and substantial increases in pH, HCO3−, and Na+ concentrations. Overall, stimulation did not considerably affect osmolality nor K+ or Cl− concentrations of saliva. An in-depth analysis of stimulation effects, using individual Δvalues, showed no correlation of Δflow rate with Δion concentrations, indicating independent regulation of acinar volume and ductal ion transport. Stimulation-induced Δ[Na+] correlated with Δ[HCO3−] and Δ[Cl−] but not with Δ[K+], indicating common regulation of ductal Na+, Cl−, and HCO3− transport. We present a robust data set of human oral saliva ion composition in healthy adults and functional insights into physiological stimulation. Our data show (i) that flow-dependence exists for Na+ and HCO3− but not for K+ and Cl− concentrations, (ii) osmolality is flow-independent, (iii) regulation of Na+, Cl−, and HCO3− transport is coupled, (iv) regulation of flow rate and ion concentrations are independent and (v) spatially separated between acini and ducts, respectively.
Carlos Asensio and the dawn of molecular microbial ecology
Authors: de Lorenzo Víctor , Baquero Fernando , Aguilar Alfredo 
Published: October 2024

AbstractAt near 50 years of the discovery of microcins, this article highlights the pivotal—but under-recognised—influence of Spanish biochemist Carlos Asensio (1925–1982) in contemporary microbiology, featuring the epistemological, sociological, and cultural impact of his scientific achievements. At a time when the intestinal microbiome is central to current biomedical research, it is due to emphasise his role in the establishment of new scientific fields that are now considered fundamental. Despite his premature death at the peak of his conceptual and experimental creativity, many of his ideas about microbial communication in complex communities inspired a generation of researchers and opened new topics reach to this day. Asensio was also a trailblazer in Spain, advocating for fundamental research within the socio-economic context of his time. He foresaw the shift towards what is now termed the knowledge-based bioeconomy, recognised the need for multidisciplinary research teams, and advocated integration science into societal and political agendas. These facets became evident during his research on microcins, low molecular weight bioactive compounds produced by enterobacteria. These molecules were hypothesised as mediators of microbial interactions in the human gut and were considered potential new antibiotics and even antitumoral agents. His research mobilised young talent and attracted unprecedented resources in Spain during the late 1970s–early 1980s. It underscored the medical value of microbial ecology and exemplified the benefits of collaboration between academia and industry. Asensio played a pivotal role in the emergence of molecular microbial ecology as a research discipline and its foundational and applied significance in biotechnology.
Automated Neural Architecture Search for Cardiac Amyloidosis Classification from [18F]-Florbetaben PET Images
Authors: Bargagna Filippo , Zigrino Donato , De Santi Lisa Anita , Genovesi Dario , Scipioni Michele , Favilli Brunella , Vergaro Giuseppe , Emdin Michele , Giorgetti Assuero , Positano Vincenzo , Santarelli Maria Filomena 
Published: October 2024

AbstractMedical image classification using convolutional neural networks (CNNs) is promising but often requires extensive manual tuning for optimal model definition. Neural architecture search (NAS) automates this process, reducing human intervention significantly. This study applies NAS to [18F]-Florbetaben PET cardiac images for classifying cardiac amyloidosis (CA) sub-types (amyloid light chain (AL) and transthyretin amyloid (ATTR)) and controls. Following data preprocessing and augmentation, an evolutionary cell-based NAS approach with a fixed network macro-structure is employed, automatically deriving cells’ micro-structure. The algorithm is executed five times, evaluating 100 mutating architectures per run on an augmented dataset of 4048 images (originally 597), totaling 5000 architectures evaluated. The best network (NAS-Net) achieves 76.95% overall accuracy. K-fold analysis yields mean ± SD percentages of sensitivity, specificity, and accuracy on the test dataset: AL subjects (98.7 ± 2.9, 99.3 ± 1.1, 99.7 ± 0.7), ATTR-CA subjects (93.3 ± 7.8, 78.0 ± 2.9, 70.9 ± 3.7), and controls (35.8 ± 14.6, 77.1 ± 2.0, 96.7 ± 4.4). NAS-derived network performance rivals manually determined networks in the literature while using fewer parameters, validating its automatic approach’s efficacy.
The Social Implication of a “mixed” Ceramic Assemblage: Understanding the Predynastic Community of Nag el-Qarmila (First Nile Cataract, Egypt) Through Pottery Technology
Authors: Bajeot Jade , Ownby Mary , Gatto Maria Carmela 
Published: October 2024

AbstractThis article investigates the social landscape of the First Nile Cataract region in the 4th mill. BCE through the application of petrographic and technological approaches to pottery analysis. The study focuses on the ceramic assemblages from the settlement WK15 and the necropolis WK14 at Nag el-Qarmila and identifies for the first time three technical traditions along with their synchronic and diachronic interaction. It provides new data on the more ancient phases of the so-called Naqadan productions and their relationship with the Shale Ware and the Nubian Black-Mouthed Ware. The results allow us to better frame the mixed assemblage that characterizes a site far from the elite centers and where relations between mobile and sedentary groups were, therefore, more fluid. The data obtained also constitute a comparative nucleus for the growth of technological and petrographic studies at other sites and periods, and the numerous insights that emerged will be useful for directing future research.
A Robotic Clamped-Kinematic System to Study Knee Ligament Injury
Authors: Herve Ophelie M. , Flanagan Will , Kanetis Jake , Mooney Bailey , Kremen Thomas J. , McAllister David R. , Clites Tyler R. 
Published: October 2024

Abstract
Knee ligament injury is among the most common sports injuries and is associated with long recovery periods and low return-to-sport rates. Unfortunately, the mechanics of ligament injury are difficult to study in vivo, and computational studies provide limited insight. The objective of this study was to implement and validate a robotic system capable of reproducing natural six degree-of-freedom clamped-kinematic trajectories on human cadaver knees (meaning that positions and orientations are rigidly controlled and resultant loads are measured). To accomplish this, we leveraged the field’s recent access to high-fidelity bone kinematics from dynamic biplanar radiography (DBR), and implemented these kinematics in a coordinate frame built around the knee’s natural flexion–extension axis. We assessed our system’s capabilities in the context of ACL injury, by moving seven cadaveric knee specimens through kinematics derived from walking, running, drop jump, and ACL injury. We then used robotically simulated clinical stability tests to evaluate the hypothesis that knee stability would be only reduced by the motions intended to injure the knee. Our results show that the structural integrity of the knee was not compromised by non-injurious motions, while the injury motion produced a clinically relevant ACL injury with characteristic anterior and valgus instability. We also demonstrated that our robotic system can provide direct measurements of reaction loads during a variety of motions, and facilitate gross evaluation of ligament failure mechanisms. Clamped-kinematic robotic evaluation of cadaver knees has the potential to deepen understanding of the mechanics of knee ligament injury.
Ozone-loaded bacterial cellulose hydrogel: a sustainable antimicrobial solution for stone cleaning
Authors: Sonaglia Erica , Schifano Emily , Augello Simone , Sharbaf Mohammad , Marra Fabrizio , Montanari Arianna , Dini Luciana , Sarto Maria Sabrina , Uccelletti Daniela , Santarelli Maria Laura 
Published: October 2024

AbstractThe use of biocide-loaded hydrogels has recently been exploited for cleaning the biological attacks of cultural heritage and architectural stone materials. However, considering the drawbacks of traditional biocides, and the high costs of synthetic polymers, growing research for innovative and sustainable solutions are taking place. The aim of this work is to explore a bacterial cellulose (BC) hydrogel functionalized with ozone as a renewable, biodegradable, and easy-to-use antimicrobial remedy for stone biodeterioration. The BC microstructure was characterized by Field Emission-Scanning Electron Microscopy observation and high crystallinity was detected by X-ray diffraction analysis. Ozonated BC (OBC) hydrogels were tested against selected biodeteriogenic microorganisms in water suspension abolishing their viability, with its complete suppression after a 10-min and a 24-h treatment with OBC, for bacterial and fungal spores, respectively. Furthermore, the OBC was assessed on contaminated marble, brick, and biocalcarenitic stone specimens for simulating in situ conditions. A 100% reduction of microbial viability after a 24-h treatment was obtained. Successively, the shelf-life of the hydrogel and the antimicrobial activity were also evaluated after 30 days, demonstrating a subsequent cleaning efficiency along time. This research highlights the potential of the new ozonated BC hydrogel as a green and highly effective antimicrobial treatment, with advantages in sustainability.
Intentional identity, mental files, and coordination: a DRT account of anaphora in attitude contexts
Authors: Fujikawa Naoya 
Published: October 2024

AbstractThis paper proposes a semantics of anaphora in attitude contexts within the framework of Discourse Representation Theory (DRT). The paper first focuses on intentional identity, a special kind of cross-attitudinal anaphora. Based on the DRT semantics of attitude reports summarized by Kamp et al. (in: D. Gabbay and F. Guenthner (Eds.), Handbook of philosophical logic, 2011), the author proposes a semantics of intentional identity that implements the following two ideas: (1) indefinites and pronouns appearing in attitude contexts introduce metadiscourse referents, which represent one’s mental files and record appearances of discourse referents in attitude contexts; and (2) what underlies the relevant kind of anaphoric links between indefinites and pronouns across attitude contexts is the coordination relation between mental files, which is represented by using metadiscourse referents. Next, the paper expands the semantics to cover de re anaphora, in which an anaphoric pronoun in an attitude context takes as its antecedent an expression appearing outside any attitude context.
Assimilation and morpheme boundaries in Mgira
Authors: Brown Jason , Meyer Johanna 
Published: October 2024

AbstractPhonological assimilation phenomena are often restricted by morphological boundaries. This paper presents data from Mgira in order to argue that both root boundaries and affix boundaries serve to block assimilation processes such as nasal place assimilation and postnasal voicing. The result is that assimilation may obtain, but only within morphemes, not across them. This is analysed as confirmation of the power of the CrispEdge constraints proposed by Itô and Mester (1999) to take precedence over general markedness constraints, blocking feature spreading across morphological boundaries. The analysis is claimed to yield the same effect as a Morpheme Structure Constraint, limiting the force of a constraint to within the boundaries of morphemes.
Highly divergent satellitomes of two barley species of agronomic importance, Hordeum chilense and H. vulgare
Authors: Gálvez-Galván Ana , Barea Lorena , Garrido-Ramos Manuel A. , Prieto Pilar 
Published: October 2024

AbstractIn this paper, we have performed an in-depth study of the complete set of the satellite DNA (satDNA) families (i.e. the satellitomes) in the genome of two barley species of agronomic value in a breeding framework, H. chilense (H1 and H7 accessions) and H. vulgare (H106 accession), which can be useful tools for studying chromosome associations during meiosis. The study has led to the analysis of a total of 18 satDNA families in H. vulgare, 25 satDNA families in H. chilense (accession H1) and 27 satDNA families in H. chilense (accession H7) that constitute 46 different satDNA families forming 36 homology groups. Our study highlights different important contributions of evolutionary and applied interests. Thus, both barley species show very divergent satDNA profiles, which could be partly explained by the differential effects of domestication versus wildlife. Divergence derives from the differential amplification of different common ancestral satellites and the emergence of new satellites in H. chilense, usually from pre-existing ones but also random sequences. There are also differences between the two H. chilense accessions, which support genetically distinct groups. The fluorescence in situ hybridization (FISH) patterns of some satDNAs yield distinctive genetic markers for the identification of specific H. chilense or H. vulgare chromosomes. Some of the satellites have peculiar structures or are related to transposable elements which provide information about their origin and expansion. Among these, we discuss the existence of different (peri)centromeric satellites that supply this region with some plasticity important for centromere evolution. These peri(centromeric) satDNAs and the set of subtelomeric satDNAs (a total of 38 different families) are analyzed in the framework of breeding as the high diversity found in the subtelomeric regions might support their putative implication in chromosome recognition and pairing during meiosis, a key point in the production of addition/substitution lines and hybrids.
Sharp Trace and Korn Inequalities for Differential Operators
Authors: Diening Lars , Gmeineder Franz 
Published: October 2024

AbstractWe establish sharp trace and Korn-type inequalities that involve vectorial differential operators, the focus being on situations where global singular integral estimates are not available. Starting from a novel approach to sharp Besov boundary traces by Riesz potentials and oscillations that equally applies to $$p=1$$

p
=
1

, a case difficult to be handled by harmonic analysis techniques, we then classify boundary trace- and Korn-type inequalities. For $$p=1$$

p
=
1

and so despite the failure of the Calderón-Zygmund theory, we prove that sharp trace estimates can be systematically reduced to full k-th order gradient estimates. Moreover, for $$1<p<\infty $$

1
<
p
<
∞

, where sharp trace estimates yield Korn-type inequalities on smooth domains, we show for the basically optimal class of John domains that Korn-type inequalities persist – even though the reduction to global Calderón-Zygmund estimates by extension operators might not be possible.
The perils of cybersecurity regulation
Authors: Hodgins Mark W. 
Published: October 2024

AbstractCybersecurity is a major U.S. policy issue with a sizable coalition advocating for greater government intervention due to perceived information challenges. Most proposals, however, do not assess whether the prescription actually improves the transmission of relevant knowledge and appropriately channels motivations towards producing socially optimal cybersecurity. In fact, knowledge and incentive problems associated with regulatory regimes may imperil the market’s ability to innovate, which is critical due to the dynamics of cyberspace. I employ Israel Kirzner’s (University of Chicago Press, 1985) market process framework to investigate these regulatory perils, highlighting analogous policies that have resulted in worse information security outcomes. Incorrect policy prescriptions, regime uncertainty, procedural rigidity, increased barriers to entry, and perverse incentives are among the leading threats to cybersecurity market entrepreneurialism from state intervention.
The role of causal manipulability in the manifestation of time biases
Authors: Hodroj Batoul , Latham Andrew J. , Miller Kristie , Pedersen Rasmus , Wang Danqi 
Published: October 2024

AbstractWe investigate the causal manipulability hypothesis, according to which what partly explains (a) why people tend to prefer negative events to be in their further future rather than their nearer future and positive events to be in their nearer future rather than their further future and (b) why people tend to prefer that negative events be located in their past not their future and that positive events be located in their future not their past, is that people tend to discount the value of events they are less able causally to manipulate. If people discount the value of events they are less able to causally manipulate, then since often temporally nearer future events are more causally manipulable than further ones, and since future events are manipulable whereas past ones aren’t, this could explain both (a) and (b). In turn, if the causal manipulability hypothesis is correct, this might suggest that insofar as people’s preferences are explained in this manner, those preferences are rationally permissible, since relative causal manipulability is normatively relevant. Thus, ascertaining whether the causal manipulability hypothesis is true may shed light on the normative status of such preferences. We investigate the cognitively mediated version of the causal manipulability hypothesis, according to which people’s consciously held beliefs about the relative causal manipulability of events explains (a) and (b). Contrary to expectations, we found no evidence in favour of this view. We suggest that either relative causal manipulability plays an explanatory role, but one that is not cognitively mediated, or that it does not play any role, even if it is sometimes associated with other factors, such as probability, that may play a role in explaining some time biased preferences.
Influence of tailing dust contamination in drinking water on the health status of goats in subtropical Regions
Authors: Serakalala William Ntshete , Mdletshe Zwelethu Mfanafuthi 
Published: October 2024

AbstractThe contamination of river systems by tailing dust remains a constraint to goat productivity in communal farming systems. A cross-sectional study was conducted to investigate how resource-limited households in subtropical regions assessed the effects of tailing dust contamination in drinking water on the health status of goats. In a study conducted in the Ba-Phalaborwa Local Municipality, 200 households from two villages were randomly selected and interviewed using a questionnaire. Forty-eight percent (n = 96) of the households were located in contaminated areas, while 52% (n = 104) were from uncontaminated areas. The study found that poor water quality, caused by tailing dust contamination, as well as a high incidence of diseases, were key factors affecting goat productivity. Water contamination was most severe during the hot and cool dry seasons. It was also noted that goats rely on freshwater as their primary source of supplemental water during dry seasons. Urine colour, oedema of the eyelids, and kid survival were indicators for assessing the health status of goats. Nominal binary logistic regression revealed that water contamination was 2.96 more likely to be reported by youth compared to elderly members. Farmers who received informal education were 37 times more likely to report contamination than those who received formal education. High kid mortality as a health status indicator was 50 times less likely to be reported in uncontaminated areas. Intervention strategies for reducing the adverse health effects of tailing dust in contaminated areas should focus primarily on the health of goats during dry seasons.
Review of Innovative Cavity Designs in Metal–Insulator-Metal Waveguide-Based Plasmonic Sensors
Authors: Butt Muhammad Ali 
Published: October 2024

AbstractPlasmonic sensors utilizing metal–insulator-metal (MIM) waveguides represent a significant advancement in sensing technology due to their high sensitivity and versatility. These sensors leverage surface plasmon polaritons to detect minute changes in the surrounding environment, making them highly effective for a range of applications. For instance, they can precisely measure variations in the Refractive Index, which is crucial for monitoring chemical concentrations and biological interactions. Additionally, MIM waveguides can be adapted to sense temperature fluctuations, pressure changes, and the presence of specific gases, providing valuable insights in fields such as environmental surveillance, medical diagnostics, and industrial processes. In recent years, a variety of sensor cavity shapes have been proposed to enhance sensor performance. This review examines how these innovative geometries optimize sensor cavities to achieve unprecedented levels of resolution and sensitivity, underscoring their transformative potential across a broad spectrum of scientific and practical applications.