bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early angiographic signs of acute thrombus formation following cerebral aneurysm treatment with the Pipeline embolization device
Authors: Patel Akil , Miller Timothy R , Shivashankar Ravi , Jindal Gaurav , Gandhi Dheeraj 
Published: October 2016

Background and objectiveAcute thrombus formation following aneurysm treatment with the Pipeline embolization device (PED) is a potentially devastating complication that may result in significant thromboembolic sequelae if not promptly treated. We therefore evaluated PED cases complicated by acute thrombus formation at our institution, with an emphasis on identifying early angiographic signs that may portend this event.Materials and methodsWe retrospectively identified cases of acute thrombosis following PED placement in 100 consecutive procedures performed at our institution from a prospectively maintained clinical database. Angiographic findings were analyzed for early signs of acute thrombus formation. We also evaluated the efficacy of treatment of this complication with a glycoprotein IIb/IIIa inhibitor (abciximab), as well as the results of pre-procedure platelet inhibition testing.ResultsAcute thrombus formation was encountered in five patients following PED placement (5%). Early angiographic signs were present in all cases and included progressive stagnation of blood flow in covered side branches, occlusion of covered side branches, excessive stagnation of blood flow in the target aneurysm, as well as occlusion of the target aneurysm. These sequelae completely resolved following abciximab treatment in all five cases, with no permanent neurological morbidity or mortality. Four of the five patients had a pre-procedure P2Y12 value >200 (range 201–227).ConclusionsProgressive stagnation or occlusion of covered side branches or target aneurysm are early angiographic signs of acute thrombus formation following PED placement and should prompt immediate treatment with a glycoprotein IIb/IIIa inhibitor. Platelet inhibition testing may help identify those patients who are at an increased risk for this complication.
Estrogen receptor antagonism exacerbates cardiac structural and functional remodeling in female rats
Authors: El Hajj M. C. , Ninh V. K. , El Hajj E. C. , Bradley J. M. , Gardner J. D. 
Published: October 2016

We have previously demonstrated the cardioprotective effects of ovarian hormones against adverse ventricular remodeling imposed by chronic volume overload. Here, we assess the estrogen receptor dependence of this cardioprotection. Four groups of female rats were studied: sham-operated (Sham), volume overloaded [aortocaval fistula (ACF)], Sham treated with estrogen receptor antagonist ICI 182,780 (Sham + ICI), and ACF treated with ICI. Cardiac function was assessed temporally using echocardiogram, and tissue samples were collected at 5 days and 6 wk postsurgery. All rats with volume overload had significantly increased cardiac output (96 ± 32 ml/min for ACF and 108 ± 11 ml/min for ACF + ICI vs. 31 ± 2 for Sham, P < 0.05). At 6 wk, volume overload induced significant left ventricular (LV) hypertrophy in both untreated and treated ACF groups. Both ACF groups developed significantly increased LV end-diastolic diameter (LVEDD), indicating LV dilatation, with the ACF + ICI group having the greatest increase (340%, relative to Sham). Ejection fraction was significantly reduced in the ACF + ICI group (23% reduction) at 6 wk postsurgery compared with untreated ACF ( P < 0.05). Interstitial collagen staining was significantly reduced by volume overload, with estrogen receptor antagonism causing greater collagen loss at both 5 days and 6 wk postsurgery. Furthermore, volume overload induced a significant increase in LV wall stress only in rats treated with estrogen antagonist. These data indicate that estrogen receptor signaling is essential for sex hormone-dependent cardioprotection against adverse remodeling. The maintenance of myocardial extracellular matrix collagen appears to play a key role in this cardioprotection.NEW & NOTEWORTHY We assessed the estrogen receptor (ER) dependence of female-specific cardioprotection using a rat model of chronic volume-overload stress. ER antagonism worsened ventricular wall stress, ventricular dilation, and cardiac dysfunction induced by volume overload. Further, blocking ERs resulted in cardiac remodeling and functional changes similar to that previously found in ovariectomized rats.
Exaggerated coronary vasoconstriction limits muscle metaboreflex-induced increases in ventricular performance in hypertension
Authors: Spranger Marty D. , Kaur Jasdeep , Sala-Mercado Javier A. , Krishnan Abhinav C. , Abu-Hamdah Rania , Alvarez Alberto , Machado Tiago M. , Augustyniak Robert A. , O’Leary Donal S. 
Published: October 2016

Increases in myocardial oxygen consumption during exercise mainly occur via increases in coronary blood flow (CBF) as cardiac oxygen extraction is high even at rest. However, sympathetic coronary constrictor tone can limit increases in CBF. Increased sympathetic nerve activity (SNA) during exercise likely occurs via the action of and interaction among activation of skeletal muscle afferents, central command, and resetting of the arterial baroreflex. As SNA is heightened even at rest in subjects with hypertension (HTN), we tested whether HTN causes exaggerated coronary vasoconstriction in canines during mild treadmill exercise with muscle metaboreflex activation (MMA; elicited by reducing hindlimb blood flow by ~60%) thereby limiting increases in CBF and ventricular performance. Experiments were repeated after α1-adrenergic blockade (prazosin; 75 µg/kg) and in the same animals following induction of HTN (modified Goldblatt 2K1C model). HTN increased mean arterial pressure from 97.1 ± 2.6 to 132.1 ± 5.6 mmHg at rest and MMA-induced increases in CBF, left ventricular dP/d tmax, and cardiac output were markedly reduced to only 32 ± 13, 26 ± 11, and 28 ± 12% of the changes observed in control. In HTN, α1-adrenergic blockade restored the coronary vasodilation and increased in ventricular function to the levels observed when normotensive. We conclude that exaggerated MMA-induced increases in SNA functionally vasoconstrict the coronary vasculature impairing increases in CBF, which limits oxygen delivery and ventricular performance in HTN.NEW & NOTEWORTHY We found that metaboreflex-induced increases in coronary blood flow and ventricular contractility are attenuated in hypertension. α1-Adrenergic blockade restored these parameters toward normal levels. These findings indicate that the primary mechanism mediating impaired metaboreflex-induced increases in ventricular function in hypertension is accentuated coronary vasoconstriction.Listen to this article’s corresponding podcast at http://ajpheart.podbean.com/e/metaboreflex-induced-functional-coronary-vasoconstriction/ .
Influencing the Tumor Microenvironment: A Phase II Study of Copper Depletion Using Tetrathiomolybdate in Patients with Breast Cancer at High Risk for Recurrence and in Preclinical Models of Lung Metastases
Authors: Chan Nancy , Willis Amy , Kornhauser Naomi , Ward Maureen M. , Lee Sharrell B. , Nackos Eleni , Seo Bo Ri , Chuang Ellen , Cigler Tessa , Moore Anne , Donovan Diana , Vallee Cobham Marta , Fitzpatrick Veronica , Schneider Sarah , Wiener Alysia , Guillaume-Abraham Jessica , Aljom Elnaz , Zelkowitz Richard , Warren J. David , Lane Maureen E. , Fischbach Claudia , Mittal Vivek , Vahdat Linda 
Published: October 2016

Abstract
Purpose: Bone marrow–derived progenitor cells, including VEGFR2+ endothelial progenitor cells (EPCs) and copper-dependent pathways, model the tumor microenvironment. We hypothesized that copper depletion using tetrathiomolybdate would reduce EPCs in high risk for patients with breast cancer who have relapsed. We investigated the effect of tetrathiomolybdate on the tumor microenvironment in preclinical models.
Experimental Design: Patients with stage II triple-negative breast cancer (TNBC), stage III and stage IV without any evidence of disease (NED), received oral tetrathiomolybdate to maintain ceruloplasmin (Cp) between 8 and 17 mg/dL for 2 years or until relapse. Endpoints were effect on EPCs and other biomarkers, safety, event-free (EFS), and overall survival (OS). For laboratory studies, MDA-LM2-luciferase cells were implanted into CB17-SCID mice and treated with tetrathiomolybdate or water. Tumor progression was quantified by bioluminescence imaging (BLI), copper depletion status by Cp oxidase levels, lysyl oxidase (LOX) activity by ELISA, and collagen deposition.
Results: Seventy-five patients enrolled; 51 patients completed 2 years (1,396 cycles). Most common grade 3/4 toxicity was neutropenia (3.7%). Lower Cp levels correlated with reduced EPCs (P = 0.002) and LOXL-2 (P &lt; 0.001). Two-year EFS for patients with stage II–III and stage IV NED was 91% and 67%, respectively. For patients with TNBC, EFS was 90% (adjuvant patients) and 69% (stage IV NED patients) at a median follow-up of 6.3 years, respectively. In preclinical models, tetrathiomolybdate decreased metastases to lungs (P = 0.04), LOX activity (P = 0.03), and collagen crosslinking (P = 0.012).
Conclusions: Tetrathiomolybdate is safe, well tolerated, and affects copper-dependent components of the tumor microenvironment. Biomarker-driven clinical trials in high risk for patients with recurrent breast cancer are warranted.
Human Microbiome Fusobacterium Nucleatum in Esophageal Cancer Tissue Is Associated with Prognosis
Authors: Yamamura Kensuke , Baba Yoshifumi , Nakagawa Shigeki , Mima Kosuke , Miyake Keisuke , Nakamura Kenichi , Sawayama Hiroshi , Kinoshita Koichi , Ishimoto Takatsugu , Iwatsuki Masaaki , Sakamoto Yasuo , Yamashita Yoichi , Yoshida Naoya , Watanabe Masayuki , Baba Hideo 
Published: October 2016

Abstract
Purpose: Fusobacterium nucleatum (F. nucleatum) is a component of the human microbiome that primarily inhabits the oral cavity. It causes periodontal disease and has also been implicated in the development of human cancers. Although there are several reports of the relationship between F. nucleatum and the clinical outcome in human cancers, its prognostic significance in esophageal cancer remains unclear.
Experimental Design: We quantified F. nucleatum DNA in 325 resected esophageal cancer specimens by qPCR. Significant pathways in F. nucleatum–positive esophageal cancer tissues were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis using microarray data.
Results: Esophageal cancer tissues contained significantly more F. nucleatum DNA than matched normal esophageal mucosa (P = 0.021; n = 60). F. nucleatum DNA was detected in 74 of 325 cases (23%). F. nucleatum DNA positivity was significantly associated with tumor stage, but not with sex, age, performance status, tobacco use, alcohol use, histology, tumor location, or preoperative treatment. F. nucleatum DNA positivity was also significantly associated with cancer-specific survival [log-rank P = 0.0039; univariate HR = 2.01; 95% confidence interval (CI), 1.22–3.23; P = 0.0068; multivariate HR = 1.78; 95% CI, 1.06–2.94; P = 0.031]. The top-ranked KEGG pathway in F. nucleatum–positive tissues was “cytokine–cytokine receptor interaction.” A significant relationship between F. nucleatum and the chemokine CCL20 was validated by IHC.
Conclusions: F. nucleatum in esophageal cancer tissues was associated with shorter survival, suggesting a potential role as a prognostic biomarker. F. nucleatum might also contribute to aggressive tumor behavior through activation of chemokines, such as CCL20. Clin Cancer Res; 22(22); 5574–81. ©2016 AACR.
THE CHANGES OF GLIAL FIBRILLARY ACID PROTEIN LEVEL IN THE DIFFERENT BRAIN AREAS OF GERBILS UNDER DEVELOPMENT, AGING AND ALPHA-KETO GLUTARATE EFFECT
Authors: Kovalchuk Yu. P. , Ushakovа H. O. 
Published: October 2016

There has been shown the change of level of astrocytes-specific glial fibrillary acid protein (GFAP) in different areas of the brain of laboratory gerbils at different stages of postnatal development, aging, and the effect of lphaketoglutarate.Studies have shown that the content of both soluble and filament forms of GFAP significantly increased during the first 30 days of postnatal development in all parts of the brain. After 30 days, the level of soluble forms of GFAP continued gradualy increase in all studied brain areas of gerbils during development and aging. Content of filament forms of GFAP in the gerbils brain after 90 days has a different tendency to change depending on the brain region. The long-term diet containing 2% alpha-ketoglutarate has been prevented overproduction of filament form of GFAP in the thalamus and hippocampus of older animals.
The research of physical and chemical, antimicrobial qualities of “Decamethoxin®” remedy
Authors: Paliy H. K. , Nazarchuk O. A. , Honchar O. O. , Kovalenko I. V. , Yatsula O. V. 
Published: October 2016

In the article there were presented the results of the research of medical physical and chemical qualities of domestic remedy “Decamethoxin®” (Decamethoxinum®), produced by Pilot Manufacturing of Institute of Organic Chemistry of National Academy of Sciences of Ukraine. It was found, that “Decamethoxin®” (DC M) remedy complies physical and chemical requirements (color, transmittance, specific optical rotation, entertainable amount of impurities, sulfate ash, heavy metals, residual amounts of organic substances, the loss of the weight no more than 4.5 %, while desiccation), established for patented exemplar of decamethoxin®. Kinetics of DCM® elution into water medium from medical materials was proved. There was found, that elution of DCM® proceeded long (for 15 days), it was like programmed controlled process, occurring due to diffusion and kinetic mechanisms.
Changes of lipid peroxidation and antioxidant protection in spontaneously hypertensive rats during experimental treatment by Ramipril and Candesartan
Authors: Marushchak A. V. , Shorikov Ye. I. , Shorikova D. V. , Tymofiychuk I. R. , Haydychuk V. S. 
Published: October 2016

This article talks about an investigation of the state of antioxidant-oxidation system for spontaneously hypertensive rats (SHR) using angiotensin-converting enzyme (ACE) drugs of short-term action. The study found that there is an increase of peroxidation processes while reducing the activity of antioxidant enzymes in the rats (SHR ), and there are features of regional influence of drugs above the level of lipid peroxidation and activity of antioxidant enzymes.
Functional state of kidneys after injection of fetal liver cells to the rats with water and salt drinking regime under the conditions of acute sublimate nephropathy
Authors: Hozhenko A. I. , Sirman V. M. , Filipets N. D. , Filipets О. О. 
Published: October 2016

The experiments on non linear white rats with acute sublimate nephropathy showed that in 7 days after intraperitoneal injection of fetal liver cells (FLC ) of rats plasma creatinine concentration decreased and proximal reabsorption of sodium ions increased independently of water or salt drinking regime. The increase of natremia in rats that used tap water excluded regulatory influence of FLC on the processes of maintenance of sodium balance under the conditions of experiment. Usage of 0.9 % NaCl solution leads to increase of glomerular filtration rate, activation of tubular transport of sodium ions with predominant elevation of reabsorption in the proximal part of nephron, reduction of sodium ions loss with urination. That points to substantial role of salt drinking regimen in additional beneficial effects of FLC on renal processes in rats with acute sublimate nephropathy.
THE INFLUENCE OF HEAVY METAL S, ORGANOPHOSPHATE PESTICIDES AND PEPTIDES ON THE ENZYME ACTIVITY OF GLUTATHIONE SYSTEM
Authors: Dmukhalska Ye. B. , Honsky Ya. I. 
Published: October 2016

The reduced glutathione content and the glutathione metabolism enzymes activity in organs and blood plasma of rats under conditions of lead acetate, copper sulphate and glyphosate in Roundup form were studied. The glutathioneperoxidase, the glutathionereductase activity and the concentration of glutathione decreases during action of heavy metals and glyphosate. It was found out that peptide exhibits antioxidant activity, the correction of the peptide and glutathione content.
Features of the damaging eff ect of chronic stress on rats’ heart of different sex
Authors: Mits I. R. , Denefil O. V. 
Published: October 2016

We investigated the effect of chronic prenatal, postnatal stress and their combination on changes in lipid peroxidation, Na+,K+-ATPase, AST and ALT, the morphological structure of the rats’ heart of different sex. It was revealed the increase in lipid peroxidation products, increase in the activity of sodium-potassium ATP ase in males and the imbalance of its work in females, an increase of ALT in males. Most increase of necrosis’ amount was observed at postnatal stress in the heart of the males, combined – in females.
The state of antioxidant system and bile formation function in the organism of rats affected by hexavalent chromium compounds
Authors: Burmas N. I. 
Published: October 2016

The affection of different age rats by potassium dichromate (3 mg/kg) was led to the violation of antioxidant system. It was confirmed by decrease of the catalase rate and reduced glutathione content in blood serum and liver of experimental animals. The most sensitive animals to the bile formation processes were immature and old animals, affected by K2Cr2O7.
Morphofunctional reorg anisation of intraorg an vessels of rat’s large salivary glands in the experimental diabetes mellitus
Authors: Yavorska-Skrabut I. M. 
Published: October 2016

There was studied the morphofunctional reorganization of the intraorgan vessels of parotid and submandibular glands of white rats with experimental hyperglycemia duration of 1, 2 and 3 months. There was established the development of hyperglycemia and presence of adaptive changes in early terms of observation that appears with constriction of small arteries while expanding of the larger caliber vascular lumen. With increasing duration of hyperglycemia, progressing of vasoconstriction and further development and deepening of destructive and sclerotic changes in vessels of parotid and submandibular glands were established.
The treatment of the lysozyme-containing drugs of patients with oral lichen planus together with dental pathology
Authors: Yeliseyeva O. V. 
Published: October 2016

Prevalence and severity of generalized parodontitis depend especially on the course of diseases affecting theoral mucosa and those ones which are characterized by involvement of the oral mucosa. These diseases include mainly lichen planus. The common pathogenic agent of generalized parodontitis and lichen planus as well as of combination of them is inflammation, as generalized parodontitis is inflammation of parardontium tissue characterized by destruction of periodontium and dental ligamentous apparatus of the alveolar bone and lichen planus is a chronic inflammatory disease of skin and oral mucosa. The purpose is to study processes of lipid peroxidation in the saliva of patients with lichen planus of the skin along with chronic generalized parodontitis.
The ways of free radical oxidation correction in the early posttraumatic period after the combined trauma of the chest and hips
Authors: Khudobyak M. M. 
Published: October 2016

The article adduces the results of the research performance of reactive oxygen in the blood and liver tissue of rats in early post-traumatic period after combined chest trauma and hips. It was established that the post-traumatic period after combined chest trauma and both hips characterizing by increasing of free oxygen radicals percentage in the blood leukocyte suspensions and liver tissue after 1 day with advanced dynamics within 3 days of the experiment. It was showed the effectiveness of the emoxipin and mexicor use in animals with combined trauma within 1 day of post-traumatic period. Comparing the data in groups of correction antihypoxants with metabolic action revealed more expressed effect of the mexicor within 3 days of the experiment, which due to the presence of succinic acid in its composition.
Impact of Proton Pump Inhibitor Use on the Comparative Effectiveness and Safety of Prasugrel Versus Clopidogrel: Insights From the Treatment With Adenosine Diphosphate Receptor Inhibitors: Longitudinal Assessment of Treatment Patterns and Events After Acute Coronary Syndrome (TRANSLATE‐ACS) Study
Authors: Jackson Larry R. , Peterson Eric D. , McCoy Lisa A. , Ju Christine , Zettler Marjorie , Baker Brian A. , Messenger John C. , Faries Douglas E. , Effron Mark B. , Cohen David J. , Wang Tracy Y. 
Published: October 2016

Background

Proton pump inhibitors (
PPI
s) reduce gastrointestinal bleeding events but may alter clopidogrel metabolism. We sought to understand the comparative effectiveness and safety of prasugrel versus clopidogrel in the context of proton pump inhibitor (
PPI
) use.

Methods and Results

Using data on 11 955 acute myocardial infarction (
MI
) patients treated with percutaneous coronary intervention at 233 hospitals and enrolled in the
TRANSLATE
‐
ACS
study, we compared whether discharge
PPI
use altered the association of 1‐year adjusted risks of major adverse cardiovascular events (
MACE
; death,
MI
, stroke, or unplanned revascularization) and Global Use of Strategies To Open Occluded Arteries (
GUSTO
) moderate/severe bleeding between prasugrel‐ and clopidogrel‐treated patients. Overall, 17% of prasugrel‐treated and 19% of clopidogrel‐treated patients received a
PPI
at hospital discharge. At 1 year, patients discharged on a
PPI
versus no
PPI
had higher risks of
MACE
(adjusted hazard ratio [
HR
] 1.38, 95% confidence interval [
CI
] 1.21‐1.58) and
GUSTO
moderate/severe bleeding (adjusted
HR
1.55, 95%
CI
1.15‐2.09). Risk of
MACE
was similar between prasugrel and clopidogrel regardless of
PPI
use (adjusted
HR
0.88, 95%
CI
0.62‐1.26 with
PPI
, adjusted
HR
1.07, 95%
CI
0.90‐1.28 without
PPI
, interaction
P
=0.31). Comparative bleeding risk associated with prasugrel versus clopidogrel use differed based on
PPI
use but did not reach statistical significance (adjusted
HR
0.73, 95%
CI
0.36‐1.48 with
PPI
, adjusted
HR
1.34, 95%
CI
0.79‐2.27 without
PPI
, interaction
P
=0.17).

Conclusions

PPI
s did not significantly affect the
MACE
and bleeding risk associated with prasugrel use, relative to clopidogrel.

Clinical Trial Registration

URL
:
https://www.clinicaltrials.gov
. Unique identifier:
NCT
01088503.