bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Identification of Five Classes of Carbapenem Resistance Genes Directly from Rectal Swabs by Use of the Xpert Carba-R Assay
Authors: Moore Nicholas M. , Cantón Rafael , Carretto Edoardo , Peterson Lance R. , Sautter Robert L. , Traczewski Maria M. 
Published: May 2017

ABSTRACT

Carbapenemase-producing organisms (CPO) have been identified by global health leaders as an urgent threat. Detection of patients with gastrointestinal carriage of CPO is necessary to interrupt their spread within health care facilities. In this multisite study, we assessed the performance of the Xpert Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects five families of carbapenemase genes (
bla
IMP
,
bla
KPC
,
bla
NDM
,
bla
OXA-48
, and
bla
VIM
) directly from rectal swab specimens. Using dual swabs, specimens from 755 patients were collected and tested prospectively. An additional 432 contrived specimens were prepared by seeding well-characterized carbapenem-susceptible and -nonsusceptible strains into a rectal swab matrix and inoculating them onto swabs prior to testing. Antimicrobial susceptibility testing, broth enriched culture, and DNA sequencing were performed by a central laboratory blind to the Xpert Carba-R results. The Xpert Carba-R assay demonstrated a positive percentage of agreement (PPA) between 60 and 100% for four targets (
bla
KPC
,
bla
NDM
,
bla
VIM
, and
bla
OXA-48
) and a negative percentage of agreement (NPA) ranging between 98.9 and 99.9% relative to the reference method (culture and sequencing of any carbapenem-nonsusceptible isolate). There were no prospective
bla
IMP
-positive samples. Contrived specimens demonstrated a PPA between 95 and 100% and an NPA of 100% for all targets. Testing of rectal swabs directly using the Xpert Carba-R assay is effective for rapid detection and identification of CPO from hospitalized patients.
Analytical and Clinical Evaluation of the PathoNostics AsperGenius Assay for Detection of Invasive Aspergillosis and Resistance to Azole Antifungal Drugs Directly from Plasma Samples
Authors: White P. Lewis , Posso Raquel B. , Barnes Rosemary A. 
Published: May 2017

ABSTRACT

With the proposal to include
Aspergillus
PCR in the revised European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) definitions for fungal disease, commercially manufactured assays may be required to provide standardization and accessibility. The PathoNostics AsperGenius assay represents one such test that has the ability to detect a range of
Aspergillus
species as well as azole resistance in
Aspergillus fumigatus
. Its performance has been validated on bronchoalveolar lavage (BAL) fluid and serum specimens, but recent evidence suggests that testing of plasma may have enhanced sensitivity over that with serum. We decided to evaluate the analytical and clinical performances of the PathoNostics AsperGenius assay for testing of plasma. For the analytical evaluations, plasma was spiked with various concentrations of
Aspergillus
genomic DNA before extraction following international recommendations, using two automated platforms. For the clinical study, 211 samples from 10 proven/probable invasive aspergillosis (IA) and 2 possible IA cases and 27 controls were tested. The limits of detection for testing of DNA extracted using the bioMérieux EasyMag and Qiagen EZ1 extractors were 5 and 10 genomes/0.5-ml sample, respectively. In the clinical study, true positivity was significantly greater than false positivity (
P
< 0.0001). The sensitivity and specificity obtained using a single positive result as significant were 80% and 77.8%, respectively. If multiple samples were required to be positive, specificity was increased to 100%, albeit sensitivity was reduced to 50%. The AsperGenius assay provided good clinical performance, but the predicted improvement of testing with plasma was not seen, possibly as a result of target degradation attributed to sample storage. Prospective testing is required to determine the clinical utility of this assay, particularly for the diagnosis of azole-resistant disease.
Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1
Authors: Franzoso Francesca D. , Seyffert Michael , Vogel Rebecca , Yakimovich Artur , de Andrade Pereira Bruna , Meier Anita F. , Sutter Sereina O. , Tobler Kurt , Vogt Bernd , Greber Urs F. , Büning Hildegard , Ackermann Mathias , Fraefel Cornel 
Published: May 2017

ABSTRACT

Adeno-associated virus 2 (AAV2) depends on the simultaneous presence of a helper virus such as herpes simplex virus 1 (HSV-1) for productive replication. At the same time, AAV2 efficiently blocks the replication of HSV-1, which would eventually limit its own replication by diminishing the helper virus reservoir. This discrepancy begs the question of how AAV2 and HSV-1 can coexist in a cell population. Here we show that in coinfected cultures, AAV2 DNA replication takes place almost exclusively in S/G
2
-phase cells, while HSV-1 DNA replication is restricted to G
1
phase. Live microscopy revealed that not only wild-type AAV2 (wtAAV2) replication but also reporter gene expression from both single-stranded and double-stranded (self-complementary) recombinant AAV2 vectors preferentially occurs in S/G
2
-phase cells, suggesting that the preference for S/G
2
phase is independent of the nature of the viral genome. Interestingly, however, a substantial proportion of S/G
2
-phase cells transduced by the double-stranded but not the single-stranded recombinant AAV2 vectors progressed through mitosis in the absence of the helper virus. We conclude that cell cycle-dependent AAV2
rep
expression facilitates cell cycle-dependent AAV2 DNA replication and inhibits HSV-1 DNA replication. This may limit competition for cellular and viral helper factors and, hence, creates a biological niche for either virus to replicate.

IMPORTANCE
Adeno-associated virus 2 (AAV2) differs from most other viruses, as it requires not only a host cell for replication but also a helper virus such as an adenovirus or a herpesvirus. This situation inevitably leads to competition for cellular resources. AAV2 has been shown to efficiently inhibit the replication of helper viruses. Here we present a new facet of the interaction between AAV2 and one of its helper viruses, herpes simplex virus 1 (HSV-1). We observed that AAV2
rep
gene expression is cell cycle dependent and gives rise to distinct time-controlled windows for HSV-1 replication. High Rep protein levels in S/G
2
phase support AAV2 replication and inhibit HSV-1 replication. Conversely, low Rep protein levels in G
1
phase permit HSV-1 replication but are insufficient for AAV2 replication. This allows both viruses to productively replicate in distinct sets of dividing cells.
Design and Implementation of a Trauma Care Bundle at a Community Hospital
Authors: Andres Ryan , Hahn Elan , de Kok Steffen , Setrak Rafi , Doyle Jeffrey , Brown Allison 
Published: May 2017

The Niagara Health System (NHS) in Ontario, Canada is comprised of three non-designated trauma center (NTC) hospitals which provide primary care to approximately 100 trauma patients annually. NTCs often lack standardized resources such as trauma surgeons, trauma-trained emergency room physicians, Advanced Trauma Life Support certified staff, trauma protocols, and other resources commonly found at designated trauma centers. Studies indicate that these differences contribute to poorer outcomes for trauma patients treated at community hospitals in Ontario, including the NTC hospitals of the NHS. In other settings healthcare checklists and bundles have proven effective in streamlining processes to ensure effective, efficient and timely patient care.Quality Improvement (QI) tools and methods were used to design, implement, and evaluate a trauma care bundle at one of the NHS's community hospitals. We assessed outcome and process measures through a chart audit of all trauma care patients in the NHS from July 2015 - November 2015. A Safety Attitudes Questionnaire (SAQ) was administered to health system staff who were involved in the pilot to assess balancing measures. Between July-November 2015, 39 patients were treated at the St. Catharines Hospital that were identified as either Canadian Triage and Acuity Scale (CTAS) I or CTAS II trauma patients. Of those 39 major trauma patients, 15 received care using the trauma care bundle, representing a 38% uptake. Patients who received care with the trauma bundle had an average Emergency Department (ED) length of stay (LOS) of 1.7 hours, compared with those patients in whom the bundle was not used, whose average ED LOS was 3.4 hours. The SAQ administered to ED physicians who used the bundle (n=10) highlighted the impact on ED patient safety. These early findings suggest that the bundle provides a substantial improvement to the current trauma care process within the Niagara Health System.
Increased T-cell Infiltration Elicited by Erk5 Deletion in a Pten-Deficient Mouse Model of Prostate Carcinogenesis
Authors: Loveridge Carolyn J. , Mui Ernest J. , Patel Rachana , Tan Ee Hong , Ahmad Imran , Welsh Michelle , Galbraith Julie , Hedley Ann , Nixon Colin , Blyth Karen , Sansom Owen , Leung Hing Y. 
Published: May 2017

Abstract
Prostate cancer does not appear to respond to immune checkpoint therapies where T-cell infiltration may be a key limiting factor. Here, we report evidence that ablating the growth regulatory kinase Erk5 can increase T-cell infiltration in an established Pten-deficient mouse model of human prostate cancer. Mice that were doubly mutant in prostate tissue for Pten and Erk5 (prostate DKO) exhibited a markedly increased median survival with reduced tumor size and proliferation compared with control Pten-mutant mice, the latter of which exhibited increased Erk5 mRNA expression. A comparative transcriptomic analysis revealed upregulation in prostate DKO mice of the chemokines Ccl5 and Cxcl10, two potent chemoattractants for T lymphocytes. Consistent with this effect, we observed a relative increase in a predominantly CD4+ T-cell infiltrate in the prostate epithelial and stroma of tumors from DKO mice. Collectively, our results offer a preclinical proof of concept for ERK5 as a target to enhance T-cell infiltrates in prostate cancer, with possible implications for leveraging immune therapy in this disease. Cancer Res; 77(12); 3158–68. ©2017 AACR.
Pulse Wave Velocity Predicts Response to Renal Denervation in Isolated Systolic Hypertension
Authors: Fengler Karl , Rommel Karl‐Philipp , Hoellriegel Robert , Blazek Stephan , Besler Christian , Desch Steffen , Schuler Gerhard , Linke Axel , Lurz Philipp 
Published: May 2017

Background
Renal sympathetic denervation seems to be less effective as a treatment for hypertension in patients with isolated systolic hypertension, a condition associated with elevated central arterial stiffness. Because isolated systolic hypertension can also be caused by wave reflection or increased cardiac output, a more differentiated approach might improve patient preselection for renal sympathetic denervation. We sought to evaluate the additional predictive value of invasive pulse wave velocity for response to renal sympathetic denervation in patients with combined versus isolated systolic hypertension.

Methods and Results

Patients scheduled for renal sympathetic denervation underwent additional invasive measurement of pulse wave velocity and pulse pressure before denervation. Blood pressure was assessed via ambulatory measurement at baseline and after 3 months. In total 109 patients (40 patients with isolated systolic hypertension) were included in our analysis. After 3 months, blood pressure reduction was more pronounced among patients with combined hypertension compared with patients with isolated systolic hypertension (systolic 24‐hour average 9.3±10.5 versus 5.0±11.5 mm Hg,
P
=0.046). However, when stratifying patients with isolated systolic hypertension by invasive pulse wave velocity, patients in the lowest tertile of pulse wave velocity had comparable blood pressure reduction (12.1±12.6 mm Hg,
P
=0.006) despite lower baseline blood pressure than patients with combined hypertension (systolic 24‐hour average 154.8±12.5 mm Hg in combined hypertension versus 141.2±8.1, 148.4±10.9, and 150.5±12.7 mm Hg, respectively, by tertiles of pulse wave velocity,
P
=0.002).

Conclusions
Extended assessment of arterial stiffness can help improve patient preselection for renal sympathetic denervation and identify a subgroup of isolated systolic hypertension patients who benefit from sympathetic modulation.
Theodor W. Adorno, Gershom Scholem, and the ‘German-Jewish Dialogue’
Authors: Fischer Lars 
Published: May 2017

Abstract
The publication of the correspondence between Theodor W. Adorno and Gershom Scholem in 2015 is a major landmark, offering fresh insights into their personalities and the remarkable intellectual relationship and growing personal friendship between them. In this short piece, some of the evidence for the intensity of the relationship between Adorno and Scholem is presented, followed by a discussion of their shared emphatic negation of the notion that any such thing as a ‘German-Jewish Dialogue’ had existed prior to 1933. Henceforth, anyone who wants to continue dismissing Scholem’s remarks about the non-existence of a ‘German-Jewish dialogue’ prior to 1933 out of hand in the cavalier fashion in which it has become common-place to do so will need to reckon not only with Scholem but also with Adorno.
Clinical Outcome After Arthroscopic Debridement and Microfracture for Osteochondritis Dissecans of the Capitellum
Authors: Bexkens Rens , van den Ende Kim I.M. , Ogink Paul T. , van Bergen Christiaan J.A. , van den Bekerom Michel P.J. , Eygendaal Denise 
Published: May 2017

Background: Various surgical treatment techniques have been developed to treat capitellar osteochondritis dissecans; however, the optimal technique remains the subject of ongoing debate. Purpose: To evaluate clinical outcomes after arthroscopic debridement and microfracture for advanced capitellar osteochondritis dissecans. Study Design: Case series; Level of evidence, 4. Methods: Between 2008 and 2015, the authors followed 77 consecutive patients (81 elbows) who underwent arthroscopic debridement and microfracture, and loose body removal if needed, for advanced capitellar osteochondritis dissecans. Seventy-one patients (75 elbows) with a minimum follow-up of 1 year were included. The mean age was 16 years (SD, ±3.3 years; range, 11-26 years) and the mean follow-up length was 3.5 years (SD, ±1.9 years; range, 1-8.2 years). Based on CT and/or MRI results, 71 lesions were classified as unstable and 4 as stable. Clinical elbow outcome (pain, function, and social-psychological effect) was assessed using the Oxford Elbow Score (OES) at final follow-up (OES range, 0-48). Range of motion and return to sports were recorded. Multivariable linear regression analysis was performed to determine predictors of postoperative OES. Results: Intraoperatively, there were 3 grade 1 lesions, 2 grade 2 lesions, 10 grade 3 lesions, 1 grade 4 lesion, and 59 grade 5 lesions. The mean postoperative OES was 40.8 (SD, ±8.0). An open capitellar physis was a predictor of better elbow outcome (5.8-point increase; P = .025), as well as loose body removal/grade 4-5 lesions (6.9-point increase; P = .0020) and shorter duration of preoperative symptoms (1.4-point increase per year; P = .029). Flexion slightly improved from 134° to 139° ( P < .001); extension deficit slightly improved from 8° to 3° ( P < .001). Pronation ( P = .47) and supination did not improve ( P = .065). Thirty-seven patients (55%) returned to their primary sport at the same level, and 5 patients (7%) returned to a lower level. Seventeen patients (25%) did not return to sport due to elbow-related symptoms, and 10 patients (13%) did not return due to non–elbow-related reasons. No complications were recorded. Conclusion: Arthroscopic debridement and microfracture for advanced capitellar osteochondritis dissecans provide good clinical results, especially in patients with open growth plate, loose body removal, and shorter duration of symptoms. However, only 62% of patients in this study returned to sports.
Anchored Transosseous-Equivalent Versus Anchorless Transosseous Rotator Cuff Repair: A Biomechanical Analysis in a Cadaveric Model
Authors: Kilcoyne Kelly G. , Guillaume Stanley G. , Hannan Catherine V. , Langdale Evan R. , Belkoff Stephen M. , Srikumaran Uma 
Published: May 2017

Background: The original approach for the repair of torn rotator cuffs involved an open technique with sutures passing through the greater tuberosity and tendon. The development of suture anchors allowed for an all-arthroscopic approach with anchor configurations attempting to re-create a transosseous fixation pattern. Presently, an arthroscopic approach can be combined with a transosseous suture configuration without using anchors. Purpose: To evaluate cyclic loading, ultimate load to failure, and the failure mechanisms of transosseous-equivalent (TOE) repair with anchors and anchorless transosseous (AT) repair of rotator cuff tears. Study Design: Controlled laboratory study. Methods: Supraspinatus tears (25 mm) were created in 20 fresh-frozen, human cadaveric shoulders, which were randomized to TOE or AT repair (10 in each group, paired experimental design). Biomechanical testing was performed with an initial preload, cyclic loading, and load to failure. Optical markers were used to monitor gap formation in 3 planes, and the failure mode was recorded. Paired t tests were used to make comparisons of biomechanical parameters between the groups. Multinomial logistic regression was used to compare failure modes between the groups. Significance was set to .05. Results: The TOE group had a significantly higher mean (±SD) ultimate failure load (578.5 ± 123.8 N) than the AT group (468.7 ± 150.9 N) ( P = .034). The TOE group also had a significantly less mean first-cycle excursion (2.97 ± 1.97 mm) than the AT group (4.70 ± 2.04 mm) ( P = .046). There were no significant differences between the groups in cyclic elongation or linear stiffness during cyclic loading. Primary modes of failure were a type 2 tendon tear with medial tendon disruption in the TOE group (7/10) and a type 1 tendon tear with lateral tendon disruption in the AT group (6/10). Conclusion: TOE repair resulted in a significantly higher mean failure load compared with AT repair in a cadaveric model. The most common modes of failure were a type 2 tendon tear in the TOE group and a type 1 tendon tear in the AT group. Clinical Relevance: A higher mean failure load in TOE versus AT constructs may come at the cost of a less favorable failure mode adjacent to medial anchors at the musculotendinous junction, potentially making revision difficult.
Fabrication of micropatterns on polypropylene films via plasma pretreatment combined with UV-initiated graft polymerization
Authors: Jiang Liu , Qian Huaming , Chen Gang , Li Chunyan , Yan Guangjun , Luo Yanling , Liu Peng , Chen Yashao 
Published: May 2017

In this study, micropatterns on polypropylene films were fabricated via plasma pretreatment and UV-initiated graft polymerization. Firstly, radio-frequency plasma, which does not significantly influence bulk attributes of substrates due to limited penetration depth, was utilized to activate polypropylene films. Then, different sizes of micropatterns of poly(hydroxyethyl methacrylate) (PHEMA) were fabricated on the polypropylene films via UV-initiated graft polymerization of hydroxyethyl methacrylate by using photo-masks. Scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle (CA) were employed to characterize changes of pristine polypropylene films and modified ones in surface morphology, roughness, hydrophilicity, free energy and the surface chemical composition. All of these confirmed the successful grafting of different sizes of PHEMA micropatterns on the polypropylene surface. Furthermore, the influence of PHEMA micropatterns on cell proliferation and cytotoxicity was evaluated in vitro. Analysis of cell behaviour indicated that PHEMA micropatterns of the appropriate size can promote cellular adhesion and proliferation, and the PHEMA-micropatterned polypropylene films had good biocompatibility. The approach presented here provides an alternative to synthesize on the surface of polypropylene films’ micropatterns with the aim of using them in a diverse array of applications.
Biventricular Repair of Double Outlet Right Ventricle: Preoperative Echocardiography and Surgical Outcomes
Authors: Meng Hong , Pang Kun-Jing , Li Shou-Jun , Hsi David , Yan Jun , Hu Sheng-Shou , Hua Zhong-Dong , Wang Hao 
Published: May 2017

Objective: To discuss the key anatomic features of double outlet right ventricle (DORV) assessed by preoperative echocardiography among patients treated with different types of biventricular repair. Methods: Surgical and echocardiographic databases were queried to identify patients who had undergone biventricular repair for DORV and had adequate preoperative echocardiographic imaging. All patients underwent pre- and postoperative echocardiography and clinical evaluation following discharge. Results: Two hundred sixty-two patients with DORV met the inclusion criteria of the study. The patients were divided into two groups—intraventricular tunnel repair (IVR) to the aorta (194 [74%] patients) or to the pulmonary artery with either concomitant arterial switch operation or double-root translocation (68 [26%] patients). Among 68 patients undergoing IVR to the pulmonary artery, 50 patients with transposition of the great arteries (TGA) type of DORV and 7 patients with remote ventricular septal defect (VSD) type underwent IVR plus arterial switch operation and 6 patients with TGA type and 5 patients with remote VSD type underwent IVR plus double-root translocation. There were three hospital deaths and one late death (overall operative mortality: 1.5%). Conclusion: Preoperative echocardiography provided crucial data to estimate the feasibility of intraventricular tunnel creation to either the aorta or the pulmonary artery and to guide the selection of either arterial switch or double-root translocation. Biventricular repair could be achieved with favorable outcomes in most patients with DORV.