bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication
Authors: Real-Hohn Antonio , Provance D. William , Gonçalves Rafael Braga , Denani Caio Bidueira , de Oliveira Andréa Cheble , Salerno Verônica P. , Oliveira Gomes Andre Marco 
Published: December 2017

AbstractTogether, the three human rhinovirus (RV) species are the most frequent cause of the common cold. Because of their high similarity with other viral species of the genus Enterovirus, within the large family Picornaviridae, studies on RV infectious activities often offer a less pathogenic model for more aggressive enteroviruses, e.g. poliovirus or EV71. Picornaviruses enter via receptor mediated endocytosis and replicate in the cytosol. Most of them depend on functional F-actin, Rab proteins, and probably motor proteins. To assess the latter, we evaluated the role of myosin light chain kinase (MLCK) and two myosin V isoforms (Va and Vb) in RV-B14 infection. We report that ML-9, a very specific MLCK inhibitor, dramatically reduced RV-B14 entry. We also demonstrate that RV-B14 infection in cells expressing dominant-negative forms of myosin Va and Vb was impaired after virus entry. Using immunofluorescent localization and immunoprecipitation, we show that myosin Va co-localized with RV-B14 exclusively after viral entry (15 min post infection) and that myosin Vb was present in the clusters of newly synthesized RNA in infected cells. These clusters, observed at 180 min post infection, are reminiscent of replication sites. Taken together, these results identify myosin light chain kinase, myosin Va and myosin Vb as new players in RV-B14 infection that participate directly or indirectly in different stages of the viral cycle.
Mechanochemical mineralization of “very persistent” fluorocarbon surfactants ‒ 6:2 fluorotelomer sulfonate (6:2FTS) as an example
Authors: Lu Mengnan , Cagnetta Giovanni , Zhang Kunlun , Huang Jun , Yu Gang 
Published: December 2017

AbstractFluorinated organic chemicals have a wide variety of industrial and consumer applications. For long time perfluorooctane sulfonate and perfluorooctanoic acid have been used as precursors for manufacture of such chemicals. However, these C8 chain compounds have been demonstrated to be toxic, persistent, and bioaccumulative, thus inducing their phase-out. Currently, C6 telomer based fluorocarbon surfactants are considered better alternatives to C8 products because of their low bioaccumulability. But, their high persistency suggests that in the near future their concentrations will increase in the environment and in industrial waste. Being a solid state non-thermal technology, mechanochemical treatment is a good candidate for the destruction of emerging C6 fluorotelomers in solid waste. In the present study, 6:2 fluorotelomer sulfonate is effectively destroyed (~100%) in rapid manner (<1 h) by high energy ball milling with KOH. Stoichiometric fluoride formation confirms its entire mineralization, assuring that no toxic by-products are generated. Reaction mechanism and kinetics indicate that effective mineralization of the perfluorinated moiety is obtained thanks to a rapid CF2 “flake-off” process through radical mechanism.
Harvesting energy from low-frequency excitations through alternate contacts between water and two dielectric materials
Authors: Yu Jian , Ma Enze , Ma Tianwei 
Published: December 2017

AbstractRecent studies have demonstrated the benefits of water-dielectric interfaces in electrostatic energy harvesting. Most efforts have been focused on extracting the kinetic energy from the motions of water drops on hydrophobic surfaces, and thus, the resulting schemes inherently prefer cases where the water drops move at a high speed, or vibrate at a high frequency. Here we report a method for directly harvesting ambient mechanical energy as electric potential energy through water droplets by making alternate contacts with CYTOP and PTFE thin films. Because CYTOP and PTFE acquire significantly different surface charge densities during contact with water, such a difference can be utilized to effectively generate electricity. We demonstrate this concept using prototype devices fabricated on silicon substrates with a simple procedure. In the experiments conducted, a water drop of 400 μL alone could generate a peak open-circuit voltage of 42 V under a 0.25 Hz vibration. Under a 2.5 Hz vibration, the peak open-circuit voltage reached 115 V under an external bias of 8 V. The demonstrated efficiency is orders of magnitude higher than those of existing devices of similar dimensions.
Mismatched filter for transmit waveform with frequency notches
Authors: Liu Aifei , Lim Yee Siang , Teh Kah Chan , Gao Caicai 
Published: December 2017

In order to reduce radar interference on communication systems within shared bands, the radar transmit waveform forms frequency notches in the shared bands. However, because of the notches, the auto‐correlation of the transmit waveform with frequency notches leads to high‐range sidelobes. In order to achieve low‐range sidelobes, the authors propose a mismatched filter. The mismatched filter for the transmit waveform with frequency notches has the same range sidelobes as the auto‐correlation of the transmit waveform without frequency notches at the expense of a signal‐to‐noise ratio (SNR) loss. Simulation and analysis results demonstrate that the proposed mismatched filter always has low sidelobes at the cost of a SNR loss. In addition, simulation results show that the SNR loss is acceptable even for the deep notches (i.e. 20 dB), when the ratio of the waveform energy in the shared bands to the total waveform energy is moderate (i.e. <8%). However, the SNR loss increases rapidly with the depth of notches, when the ratio is large.
Refractory Chest Pain or Treating Cardiologist's Bane: A Case Report
Authors: Hegde Anupama , Tibdewal Abhinay , Prakash Vadagenalli , Sahoo Sarthak 
Published: December 2017

AbstractMicrovascular angina is caused by dysfunction of small-resistance coronary arteries (< 500 µm) and is of heterogenous origin. The major epicardial coronaries are normal and commonly seen in women. Prognosis is variable, with disabling angina in many patients and can be a cause of mortality, especially in those who are refractory to treatment. In this background, we present a case of 56-year-old post valve replacement with normally functioning aortic valve and recurrent episodes of microvascular angina. The patient had normal epicardial coronaries. She had recurrent episodes of angina refractory to various antianginals associated with hemodynamic instability. Microvascular angina can curtail routine activity, frequent hospitalization, and repeated noninvasive and invasive investigations. Thus, it is a high social and economical disease, especially pertinent to women.
Correlation of Treadmill Stress Test with Coronary Angiography to Predict Coronary Artery Disease in Males versus Females
Authors: Maneesh Kumar B. , Bhuvaneshwari Edavaluru 
Published: December 2017

Background Treadmill stress test (TMT) is the most commonly performed stress test for diagnosis of coronary artery disease (CAD) in outpatient setting. The present study was conducted to find correlation of TMT with coronary angiography (CAG) in males versus female patients.
Methods Total 422 patients who had undergone CAG and TMT were enrolled in present study. TMT test was done using Bruce protocol, and results were classified as inconclusive, low, intermediate, and high probability for inducible ischemia.
Results Of 422 patients enrolled in the present study, 290 (69%) were males and 132 (31%) were females. Clinical presentation was angina on exertion CCS class II–III in 302 (71.6%) patients and angina equivalent in 124 (29.4%) patients. Out of these, 211 (50.2%) were diabetic and 308 (72.9%) were hypertensive. TMT was inconclusive, low, intermediate, and high probability for inducible ischemia in 35 (8.2%), 40 (9.7%), 19 (4.5%), and 328 (77.7%) patients, respectively. CAGs were normal in 149 (35.4%) patients and abnormal in 273 (64.6%) patients. Out of 328 patients with strongly positive TMT, 104 (31.7%) had normal coronaries, 56 (17%) had mild CAD, while 75 (22.8%) had SVD. LMCA disease, DVD, and TVD were found in 5 (1.5%), 48 (14.6%), and 40 (12.1%) patients, respectively. Of 132 female patients, 70 (53%) were found to have CAD and 62 (47%) had normal coronaries on CAG. Of 290 males, 241(83.1%) were found to have CAD and 49 (16.8%) had normal coronaries on CAG. Positive predictive value (PPV) in females was 53% and in males was 83%. Subgroup analysis showed that if there was high-probability TMT along with older age (p < 0.00), male sex (p < 0.01), and diabetes (p < 0.00), chances of having CAD were more.
Conclusion PPV of positive TMT in female is 53% and in male is 83%. High-probability TMT along with the older age, male sex, and presence of diabetes mellitus (DM) makes the probability of having CAD is high.
Options in Repositioning the Asymmetric Brow from Paralysis and Trauma
Authors: Wang Lexie , Han Reintine , Mourad Moustafa , Ducic Yadranko , Lee Thomas 
Published: December 2017

AbstractBrow asymmetry is a relatively common occurrence, especially in the facial plastic surgeon's office. In this article, the authors review causes of brow asymmetry, pertinent anatomy, and a litany of treatment options including nonsurgical and surgical approaches. They offer an algorithm that summarizes the various techniques for management with the goal of tailoring treatment tactics to the individual patient's needs.
Secondary Repair of Posttraumatic Enophthalmos and Extraocular Movement Disorders
Authors: Ali Kausar , Schultz Kelly , Marx Douglas , Buchanan Edward , Hollier Larry 
Published: December 2017

AbstractEnophthalmos, or recession of the eye posteriorly and inferiorly, is a potential sequela of orbital trauma and a source of significant cosmetic and functional concern. Late enophthalmos occurs when early reconstruction of the bony orbit fails to completely restore normal orbital shape and volume, resulting in aesthetic deformity and persistent diplopia. In this article, we provide a framework for evaluation of posttraumatic enophthalmos and outline the surgical principles of secondary repair necessary to optimize globe position. With implementation of proper craniofacial exposure, osteotomy, and orbital reconstruction, surgeons may achieve significant improvement in both the aesthetic and functional sequelae of enophthalmos.
Begutachtung von Hörschäden
Authors: Thielker Jovanna , Schneider Gerlind , Guntinas-Lichius Orlando 
Published: December 2017

ZusammenfassungDie Beurteilung von Hörschäden ist eine häufige Fragestellung in HNO-Gutachten, hierzu zählt die Bewertung von Hörschäden als Unfallfolge,
z. B. durch eine Kopfverletzung oder ein akut aufgetretenes Schalltrauma. Schwerhörigkeit als Folge einer beruflich bedingten chronischen
Lärmeinwirkung gehört zu den am häufigsten bewerteten Berufskrankheiten überhaupt. Dieser Beitrag beleuchtet die Schadensbewertung und
entsprechende Rechtsgrundlagen.
Entwicklung und Evaluation eines ärztlich-psychotherapeutischen Befundberichts. Option für neue Zugangswege in die Suchtrehabilitation
Authors: Brünger Martin , Krüger Tim , Missel Peter , Feige Lothar , Spyra Karla 
Published: December 2017

Zusammenfassung
Fragestellung Trotz Leitlinien-Empfehlungen für die Rehabilitation von Abhängigkeitserkrankungen ist die Inanspruchnahme von Entwöhnungsbehandlungen niedrig und war zwischen 2010 und 2015 sogar um 20% rückläufig. Bislang ist der Zugang zur Suchtrehabilitation im Unterschied zur somatischen und psychosomatischen Rehabilitation nicht direkt aus dem ambulanten Sektor möglich, sondern erfolgt im Regelfall über Suchtberatungsstellen. Es scheinen schnittstellenbedingte und weitere Barrieren im Zugang zu Entwöhnungsbehandlungen zu bestehen. Ziel der qualitativen Studie war die Entwicklung und Evaluation eines ärztlich-psychotherapeutischen Befundberichts, der ergänzend im direkten Zugang zur Suchtrehabilitation eingesetzt werden kann.
Material und Methoden Literatur-basiert wurde eine Entwurfsversion des Befundberichts konzipiert, welche nachfolgend im Rahmen eines mehrstufigen Delphi-Verfahrens durch 15 Experten aus allen relevanten Bereichen und Professionen evaluiert wurde.
Ergebnisse Aufbau, Inhalt und Verständlichkeit des Befundberichts wurden positiv bewertet, die Schaffung eines ergänzenden Zugangswegs in die Rehabilitation Abhängigkeitskranker begrüßt. Nach mehrfacher Überarbeitung und Rückkopplung mit den Experten konnte eine Pilotversion konsentiert werden.
Diskussion und Schlussfolgerung Eine Pilotversion des Befundberichts liegt vor, welche zukünftig insbesondere durch niedergelassene Psychiater, Psychotherapeuten und Hausärzte genutzt werden kann. Auf Grundlage einer qualifizierten ärztlich-psychotherapeutischen Befundung könnten neue Zugangswege in die Suchtrehabilitation für bislang nicht hinlänglich erreichte Patientengruppen mit Rehabilitationsbedarf erschlossen werden.
A distributional semantic approach to the periodization of change in the productivity of constructions
Authors: Perek Florent , Hilpert Martin 
Published: December 2017

AbstractThis paper describes a method to automatically identify stages of language change in diachronic corpus data, combining variability-based neighbour clustering, which offers objective and reproducible criteria for periodization, and distributional semantics as a representation of lexical meaning. This method partitions the history of a grammatical construction according to qualitative stages of productivity corresponding to different semantic sets of lexical items attested in it. Two case studies are presented. The first case study on thehell-construction (“Verbthe hell out ofNP”) shows that the semantic development of a construction does not always match that of its quantitative aspects, like token or type frequency. The second case study on theway-construction compares the results of the present method with those of collostructional analysis. It is shown that the former measures semantic changes and their chronology with greater precision. In sum, this method offers a promising approach to exploring semantic variation in the lexical fillers of constructions and to modelling constructional change.
Again on the revolutionary subject: problematising class and subalternity in Gramsci on Tahrir
Authors: Roccu Roberto 
Published: December 2017

SUMMARY
Roberto Roccu’s intervention provides a detailed reading of the concepts of subalternity, common sense and passive revolution as employed in Gramsci on Tahrir. Roccu calls for a more sustained and careful reading of how ‘subaltern agency’ is invoked and performed in revolutionary upheavals.
Utilization of an intermediate of the methylerythritol phosphate pathway, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate, as the prenyl donor substrate for various prenyltransferases
Authors: Hayashi Yoshifumi , Ito Tomokazu , Yoshimura Tohru , Hemmi Hisashi 
Published: December 2017

Abstract
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate (HMBPP) is an intermediate of the methylerythritol phosphate pathway. Utilization of HMBPP by lycopene elongase from Corynebacterium glutamicum, which is a UbiA-family prenyltransferase responsible for C50 carotenoid biosynthesis, was investigated using an Escherichia coli strain that contained the exogenous mevalonate pathway as well as the carotenoid biosynthetic pathway. Inhibition of the endogenous methylerythritol phosphate pathway resulted in loss of the production of C50 carotenoid flavuxanthin, while C40 lycopene formation was retained. Overexpression of E. coli ispH gene, which encodes HMBPP reductase, also decreased the production of flavuxanthin in E. coli cells. These results indicate the preference of lycopene elongase for HMBPP instead of the previously proposed substrate, dimethylallyl diphosphate. Furthermore, several (all-E)-prenyl diphosphate synthases, which are classified in a distinct family of prenyltransferase, were demonstrated to accept HMBPP, which implies that the compound is more widely used as a prenyl donor substrate than was previously expected.
A crude sword bean (Canavalia gladiata) extract is gelated by cooling
Authors: Nishizawa Kaho , Arii Yasuhiro 
Published: December 2017

Abstract
White sword bean (Canavalia gladiata) seeds have the potential to be utilized in the manufacturing of processed foods owing to their high protein and carbohydrate content. Our previous reports explored the use of the sword bean as a source of food materials by preparing extracts in distilled water. In the present study, we found that one such extract can be gelated by cooling. The gelling substances were extracted by boiling and simultaneously stirring a suspension containing ground beans. Few proteins were present in the gelated extract. We also examined the conditions under which gelation occurred and the gel melting temperature. The extract gelated at temperatures below 10 °C, and the resulting gel melted at those above 65 °C. This is the first report that gelling substances can be extracted from sword beans in large quantities. We expect that this gelling agent can be used for the production of processed foods.
Clinical Practice Guideline for Physical Therapy Assessment and Treatment in Patients With Nonspecific Neck Pain
Authors: Bier Jasper D , Scholten-Peeters Wendy G.M , Staal J Bart , Pool Jan , van Tulder Maurits W , Beekman Emmylou , Knoop Jesper , Meerhoff Guus , Verhagen Arianne P 
Published: December 2017

Abstract
The Royal Dutch Society for Physical Therapy (KNGF) issued a clinical practice guideline for physical therapists that addresses the assessment and treatment of patients with nonspecific neck pain, including cervical radiculopathy, in Dutch primary care. Recommendations were based on a review of published systematic reviews.
During the intake, the patient is screened for serious pathologies and corresponding patterns. Patients with cervical radiculopathy can be included or excluded through corresponding signs and symptoms and possibly diagnostic tests (Spurling test, traction/distraction test, and Upper Limb Tension Test). History taking is done to gather information about patients’ limitations, course of pain, and prognostic factors (eg, coping style) and answers to health-related questions.
In case of a normal recovery (treatment profile A), management should be hands-off, and patients should receive advice from the physical therapist and possibly some simple exercises to supplement “acting as usual.”
In case of a delayed/deviant recovery (treatment profile B), the physical therapist is advised to use, in addition to the recommendations for treatment profile A, forms of mobilization and/or manipulation in combination with exercise therapy. Other interventions may also be considered. The physical therapist is advised not to use dry needling, low-level laser, electrotherapy, ultrasound, traction, and/or a cervical collar.
In case of a delayed/deviant recovery with clear and/or dominant psychosocial prognostic factors (treatment profile C), these factors should first be addressed by the physical therapist, when possible, or the patient should be referred to a specialist, when necessary.
In case of neck pain grade III (treatment profile D), the therapy resembles that for profile B, but the use of a cervical collar for pain reduction may be considered. The advice is to use it sparingly: only for a short period per day and only for a few weeks.
Estimating minimally important differences for the PROMIS pain interference scales: results from 3 randomized clinical trials
Authors: Chen Chen X. , Kroenke Kurt , Stump Timothy E. , Kean Jacob , Carpenter Janet S. , Krebs Erin E. , Bair Matthew J. , Damush Teresa M. , Monahan Patrick O. 
Published: December 2017

Abstract
Minimally important difference (MID) refers to the smallest meaningful difference that carries implications for patient care. Minimally important differences are necessary to help interpret patient-reported pain outcomes in research and clinical practice. The PROMIS pain interference scales were validated across diverse samples; however, more information about their MIDs could improve their interpretability. The purpose of this study was to estimate MIDs for 4 fixed-length PROMIS pain interference scales, including the 6-item Pain Short Form and the 4-, 6-, and 8-item pain interference scales used in the PROMIS profile instruments. Data were analyzed from 3 randomized controlled trials (N = 759). The 3 samples, respectively, consisted of patients with chronic low back pain (n = 261), chronic back pain or hip/knee osteoarthritis pain (n = 240), and a history of stroke (n = 258). For each sample, anchor- and distribution-based approaches were used to estimate MIDs. Standard error of measurement and effect sizes were used as distribution-based MID estimates. Anchor-based MID estimates were established by mapping PROMIS pain interference scores onto established anchor measures, including the Brief Pain Inventory, and retrospective and prospective global ratings of change. The distribution- and anchor-based MID estimates showed convergence. For the pain samples, MID estimates ranged from 2 to 3 T-score points. For the nonpain sample, MID estimates ranged from 3.5 to 4.5 T-score points. The MID estimates were comparable across the 4 fixed-length scales. These MIDs can be used to evaluate treatment effects in research and clinical care and to calculate estimates for powering clinical trials.
Improving the annotation of the Heterorhabditis bacteriophora genome
Authors: McLean Florence , Berger Duncan , Laetsch Dominik R. , Schwartz Hillel T. , Blaxter Mark 
Published: December 2017

AbstractGenome assembly and annotation remains an exacting task. As the tools available for these tasks improve, it is useful to return to data produced with earlier instances to assess their credibility and correctness. The entomopathogenic nematode Heterorhabditis bacteriophora is widely used to control insect pests in horticulture. The genome sequence for this species was reported to encode an unusually high proportion of unique proteins and a paucity of secreted proteins compared to other related nematodes. We revisited the H. bacteriophora genome assembly and gene predictions to ask whether these unusual characteristics were biological or methodological in origin. We mapped an independent resequencing dataset to the genome and used the blobtools pipeline to identify potential contaminants. While present (0.2% of the genome span, 0.4% of predicted proteins), assembly contamination was not significant. Re-prediction of the gene set using BRAKER1 and published transcriptome data generated a predicted proteome that was very different from the published one. The new gene set had a much reduced complement of unique proteins, better completeness values that were in line with other related species’ genomes, and an increased number of proteins predicted to be secreted. It is thus likely that methodological issues drove the apparent uniqueness of the initial H. bacteriophora genome annotation and that similar contamination and misannotation issues affect other published genome assemblies.