bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and cryopreservation of adipose tissue derived stem cells from different adipose tissues in obese patients
Authors: Freitas D. , Aguiar A. , Janz F. , Milléo F. , Favero G. 
Published: September 2017

Abstract
Introduction: Adult stem cells (ASCs) are a population of tissue-resident cells that have the capacity for self-renewal and differentiation into different cell types with potential for cell therapies. New approaches for ASCs isolation, including many tissue sources and new protocols that are more effective and less expensive are under investigation. Thus this work aim is to isolate, maintain in cell culture and evaluate cryopreservation protocols for adipose derived stem cells (ADSCs) from different tissues such as subcutaneous adipose tissue, visceral mesenteric and omental visceral taken from the same individual. Material and Methods: The techniques of mechanical and enzymatic dissociation were used, in order to investigate the most appropriated method to ADSCs isolation. Dimethylsulfoxide (DMSO) and dimethylformamide (DMF) in different concentrations were tested as cryoprotectors in 24, 48 and 72 hours thawing protocols. The samples were collected from obese patients with associated diseases undergoing bariatric surgery, between 30 to 45 years old. Results: Among 10 collected samples it was possible to measure cell viability from 4 patients. The higher cell rate was obtained from the visceral tissue of omentum. Conclusion: DMSO was the more efficient cryopreservant for this condition. This adipose tissue source could be explored for ADSCs isolation and future clinical investigations.
High khat dose and long-term exposure impairs spermatogenesis: experimental study using rabbit model
Authors: Nyongesa A. , Patel N. , Wango E. , Onyango D. 
Published: September 2017

Abstract
Introduction: This study investigated short- and long-term effects of khat (Catha edulis) on hypophyseal, epididymal and testicular morphology, body weight and temperature changes and haematological parameters of rabbits. Materials and Methods: Twenty five male New Zealand White rabbits, divided into five groups were used. First four groups were administered, via intra-gastric tube, 1.5, 4.5, 13.5 and 40.5 g/kg body weight respectively of khat extract thrice a week for 8 weeks while controls received normal saline. Short-term and long-term effects were evaluated for hypophyseal, epididymal and testicular morphology, body temperature as well as body weight changes, food consumption and haematological parameters. Data on haematological parameters, body weight changes, body temperature and food consumption was done using one-way ANOVA at 95% confidence interval using SPSS version 12.0. Results: There was vacuolation in spermatogonia and spermatocytes at high doses while epididymides and hypophyses were unaffected. A significant decrease (P<0.05) in body weight of treatment groups correlated with reduced food intake with increasing doses and chronicity of exposure. Packed cell volume, red blood cell count and haemoglobin concentration decreased while white blood cell count increased with increasing doses. Conclusion: Khat extract had direct effects on spermatogenesis compounded by poor body weight gain, hyperthermia and blood volume loss.
The 9-Item Physician Documentation Quality Instrument (PDQI-9) score is not useful in evaluating EMR (scribe) note quality in Emergency Medicine
Authors: Wang Andrew , Dunlop William , Rodda Hamish , Ben-Meir Michael , Staples Margaret , Walker Katherine J. 
Published: September 2017

Summary
Background: Scribes are assisting Emergency Physicians by writing their electronic clinical notes at the bedside during consultations. They increase physician productivity and improve their working conditions. The quality of Emergency scribe notes is unevaluated and important to determine.
Objective: The primary objective of the study was to determine if the quality of Emergency Department scribe notes was equivalent to physician only notes, using the Physician Documentation Quality Instrument, Nine-item tool (PDQI-9).
Methods: This was a retrospective, observational study comparing 110 scribed to 110 non-scribed Emergency Physician notes written at Cabrini Emergency Department, Australia. Consultations during a randomised controlled trial of scribe/doctor productivity in 2016 were used. Emergency physicians and nurses rated randomly selected, blinded and de-identified notes, 2 raters per note. Comparisons were made between paired scribed and unscribed notes and between raters of each note. Characteristics of individual raters were examined. The ability of the tool to discriminate between good and poor notes was tested.
Results: The PDQI-9 tool has significant issues. Individual items had good internal consistency (Cronbach’s alpha=0.93), but there was very poor agreement between raters (Pearson’s r=0.07, p=0.270). There were substantial differences in PDQI-9 scores allocated by each rater, with some giving typically lower scores than others, F(25,206)=1.93, p=0.007. The tool was unable to distinguish good from poor notes, F(3,34)=1.15, p=0.342. There was no difference in PDQI-9 score between scribed and non-scribed notes.
Conclusions: The PDQI-9 documentation quality tool did not demonstrate reliability or validity in evaluating Emergency Medicine consultation notes. We found no evidence that scribed notes were of poorer quality than non-scribed notes, however Emergency scribe note quality has not yet been determined.Citation: Walker KJ, Wang A, Dunlop W, Rodda H, Ben-Meir M, Staples M. The 9-Item Physician Documentation Quality Instrument (PDQI-9) score is not useful in evaluating EMR (scribe) note quality in Emergency Medicine. Appl Clin Inform 2017; 8: 981–993 https://doi.org/10.4338/ACI2017052017050080
Educational Activities for Acquiring Skills in the EEES adapted Degrees
Authors: Tolosa Santiago , Hidalgo Antonio , Ojalvo Evaristo A. , Guiberteau Agustina 
Published: September 2017

<p class="Textoindependiente21"><span lang="EN-US">The main objective of this paper is to describe the performance of a serial of training activities focused on the acquisition of skills by different groups of undergraduate students related to science as a branch of knowledge. Specifically, we used those activities where students receive knowledge, such as theoretical classes (T), computational (PC) and laboratory (PL) sessions, exercises (P), ECTS tutorials and bachelor thesis (TFG). Among the different activities, it must also be highlighted the undergraduate dissertation. The activities were conducted in different courses of the Chemistry degree of the University of Extremadura, in order to obtain information from the different competences that appear in the verification report of this title. Finally, the students filled out a survey giving their opinion about the skills acquired in each of the activities.</span></p><p class="Textoindependiente21"><span lang="EN-US">The results of the experience is that students achieve most of the skills considered in the title. Likewise, the results from the activities considered show that not all skills are achieved and other activities should be considered to cover those deficiencies. The activity that most contributes to the acquisition of transversal competences are laboratory practices, while for specific skills, the activities developed with large group of students (T and P) are the most significant. TFG is the activity that best covers most of competences, especially Basic and General.</span></p><p class="Textoindependiente21"> </p>
Theoretical Biomimetics: A biological design-driven concept for creative problem-solving as applied to the optimal sequencing of active learning techniques in educational theory
Authors: Carroll La Shun L. 
Published: September 2017

<p><span lang="EN-US">The article introduces the author’s concept of applying principles of biological design to drive creative problem-solving. It provides a brief background of the field of Biomimetics, which serves as a context for the reader to appreciate how it began with an established field upon which he constructed and adapted his concept to apply to human-made intangibles. A discussion of Theoretical Biomimetics specifies the differences between it and its predecessor. The final section provides the opportunity to see Theoretical Biomimetics in detail applied to education theory to address a problem related to student learning in higher education and how best to establish optimized sequences to implement evidence-based active-learning techniques to fill a void in the literature demonstrating from nature what has worked. The originality lies in the author taking a multidisciplinary approach to synergize a sequence of existing active-learning techniques and apply them to a new area in a new way. </span></p>
Constelaciones de la memoria: sobre Nostalgia de la Luz [2010] y El Botón de Nácar [2015] de Patricio Guzmán
Authors: Zarini María Emilia 
Published: September 2017

Nostalgia de la Luz [2010] y El Botón de Nácar [2015] son parte de una trilogía a cargo del documentalista chileno Patricio Guzmán. En estas dos películas el autor se propone una reflexión sobre la memoria histórica y política de Chile. Lo singular de su propuesta son los elementos aparentemente disímiles que participan del diálogo. Es así como los pueblos originarios del sur de Chile y los desaparecidos de la dictadura de Pinochet se urden en un entramado con el desierto de Atacama, la astronomía, las mujeres buscadoras de Calama, el agua y los botones de nácar, entre otros hilos de la historia. El resultado es un tejido del tiempo, una posible relación con este, con todo aquello que implica pensarlo –historia, ciencia, ética, territorios, relaciones– o como pensamiento de lo impensable. ¿Qué elementos traman las imágenes de lo real que Guzmán propone? ¿Cuál es la potencia de estas imágenes? Si pensamos en ellas cual vórtice que interrumpe el flujo de la corriente de las imágenes-movimiento, ¿hacia dónde rota nuestro lazo con el mundo?, ¿qué nos depara creer con lo impensable?, ¿qué implica imaginar el caos? Para ensayar algunas respuestas posibles y de este modo dar cuenta de ciertos aspectos del problema de lo real en el documental contemporáneo, me propongo el análisis de estos dos films en función de tres vértices: 1) la creencia, 2) la imaginación y 3) el tiempo, considerando que estos trazan una cartografía de la memoria que los dos films de Guzmán visibilizan. Palabras clave: Documental. Memoria. Imaginación. Creencia. Tiempo
Historias cotidianas y Los rubios: dos formas de narrar el pasado
Authors: Morazzo María Virginia 
Published: September 2017

Según Gustavo Aprea (2015) hay tres modalidades de abordaje que plantean los documentales sobre la militancia que participó de los hechos ocurridos durante la Dictadura Militar Argentina (1976-1983): la primera; desde la vivencia y lo testimonial, la segunda desde la memoria y el no-olvido, y la tercera desde la posibilidad de transmisión de la experiencia de la militancia revolucionaria que construye modelos para las nuevas generaciones. Los primeros relatan la historia desde la derrota y con una lectura política, los segundos desde el trauma y con una lectura personal, y los terceros desde el reconocimiento y con una lectura profesional de los hechos. En este artículo se desarrollará el segundo grupo desde el análisis de las películas Historias cotidianas (2001) de Andrés Habegger y Los rubios (2003) de Albertina Carri, quienes reconstruyen la militancia desde lo afectivo, buscando un cierre a una etapa de sus vidas por medio de una memoria fragmentada. Si bien ambos documentales son guionizados y dirigidos por hijos de desaparecidos en la época de Terrorismo de Estado, ya desde el título se encuentra una diferencia marcada en su abordaje temático, que se visualiza también en el tratamiento estético-narrativo. Por ende, el presente artículo tiene como objetivo llevar adelante un análisis narrativo (contemplando estructura y recursos) de ambas obras y, a su vez, tratar de comprender sus mensajes, sus significados, a la luz de la teoría expuesta por múltiples estudiosos del cine documental político, con el fin último de poder establecer un punto de encuentro. Palabras clave: Documental Político. Memoria. Narración Audiovisual.
Memórias de um massacre: violência em A Guerra dos Pelados (Sylvio Back, 1971)
Authors: Kaminski Rosane 
Published: September 2017

Em 1971, no contexto dos anos de chumbo da ditadura civil-militar brasileira, o cineasta Sylvio Back lançou o seu segundo filme de longa-metragem, A Guerra dos Pelados, que versa sobre o Movimento do Contestado. A tônica do filme é pessimista e representa a opressão e o massacre sofridos pela comunidade de posseiros, tanto por parte dos fazendeiros locais, quanto pelas autoridades militares. Com tal obra, ao mesmo tempo em que edificava uma memória sobre o passado histórico brasileiro, Back posicionava-se frente às questões do seu tempo. O filme carrega uma ambiguidade bastante significativa ante seu contexto de produção, no que concerne à relação entre os grupos resistentes e a imposição do poder militar por meio do uso da violência. Partindo dessa relação metafórica entre o filme histórico e o seu tempo de produção, este artigo discute as formas de figuração da violência no filme, considerando que essas situações se distinguem por sua função dentro do enredo: algumas vezes, a violência é utilizada como instrumento de coerção, visando garantir a manutenção do poder e o predomínio das formas de dominação e exploração; noutras, a violência pode surgir como instrumento revolucionário, buscando transgredir as relações de poder que se arraigaram. Palavras-chave: BACK, Sylvio - 1937. Cinema e História. Brasil - História - Campanha do Contestado, 1912-1916. Violência.
História do Partido Comunista do Brasil (PCdoB): um balanço bibliográfico
Authors: Sales Jean Rodrigues 
Published: September 2017

O artigo apresenta um itinerário da produção histórica existente a respeito do Partido Comunista do Brasil (PCdoB). Analisa trabalhos realizados dentro e fora da universidade e aponta as principais linhas interpretativas e debates políticos que envolvem essa produção. Palavras-chave: Partido Comunista do Brasil (PCdoB). História. Historiografia
Los mapas históricos como instrumentos para la enseñanza de la historia
Authors: Parellada Cristian Abraham 
Published: September 2017

Este artículo tiene como objetivo proponer un diálogo entre las investigaciones que analizan cómo los estudiantes comprenden los acontecimientos del pasado desde la perspectiva del pensamiento histórico y las indagaciones que, desde la historia de la cartografía, han desarrollado una mirada crítica sobre los mapas. Se considera que los mapas son instrumentos de la cultura material escolar y la reflexión crítica acerca de ellos puede aportar nuevas estrategias didácticas para la comprensión historiográfica del proceso de construcción del Estado-nación. Por otra parte, al promover un diálogo con las investigaciones sobre pensamiento histórico, se busca que los estudiantes desarrollen herramientas propias del investigador para reflexionar acerca de procesos del pasado nacional y las imágenes con que se representa al territorio. Se concluye que el campo de la enseñanza de la historia escolar se beneficiaría de los desarrollos críticos en historia de la cartografía, en tanto permiten a los estudiantes comprender no sólo lo que muestra el mapa y cómo lo muestra, sino también cómo el territorio nacional era concebido y representado en otras épocas y sociedades. En síntesis, el diálogo propuesto entre ambas disciplinas intenta promover en los estudiantes la adquisición de ciertas herramientas que les permitan historiar qué muestra el mapa y cómo lo hace. Palabras clave: Mapas Históricos. Enseñanza de la Historia. Pensamiento Histórico. Representación del Territorio Nacional.
Abordagem metodológica de conservação da história e do patrimônio: o Vale do Douro
Authors: Freitas Isabel Vaz de , Cea Juan Carlos Martín , Zubizarreta Olatz Villanueva , Valdivieso Isabel del Val 
Published: September 2017

O Patrimônio em áreas rurais ou urbanas anota a passagem do tempo e da história das comunidades. Evoca memórias locais que permitem ao visitante sentir diversas emoções e passar por várias experiências. Monumentos ou construções mais simples, mais complexos ou até desaparecidos, podem ser transformados em paisagens cênicas e atrair visitantes permitindo conhecer as diferentes perspectivas com que o homem assentou nos espaços num determinado tempo. Propõe-se capturar esses momentos históricos, sobreviventes nos territórios até aos nossos dias e expor o significado estético, social, econômico e político como parte de uma construção espacial preparada para ser visitada e conhecida em comum. Retratamos um elemento de grande relevo para a Península Ibérica – o Rio Douro – e sugerimos que esta herança histórica comum, que se apresenta hoje de formas diversas, seja incluída nos itinerários de forma a revelar cenários interpretados e levar os visitantes a lugares históricos plenos de emoção. Sugerimos como metodologia a reflexão e o debate em torno da revisão da literatura, resultantes de projetos desenvolvidos sobre o tema da água desde 1996, sob a supervisão de Isabel Del Val Valdivieso e com a colaboração de numerosos pesquisadores da Península Ibérica que produziram extensa literatura sobre a temática da presença de água na história. De acordo com os resultados desses projetos, desenvolve-se uma metodologia que possa salvaguardar e comunicar o patrimônio com uma perspectiva de preservação e manutenção da memória para as futuras gerações, em particular do patrimônio desaparecido ou ainda adormecido no subsolo. Palavras-chave: Patrimônio. Significância. História. Visita.
Detection, Patterns, and Outcomes of Recurrent HPV-Positive Oropharyngeal Squamous Cell Carcinoma
Authors: Shah Rema , Wilkins Sarah G. , Safranek Conrad W. , Shah Hemali P. , Brophy Catherine , Mehra Saral 
Published: October 2024

ImportanceRecurrent human papillomavirus (HPV)–positive oropharyngeal squamous cell carcinoma (OPSCC) is a relatively rare but serious disease with little empirical data. Previous works have studied patterns of recurrence in HPV-positive OPSCC, but only one has studied truly recurrent disease as opposed to persistent disease, and no work systematically analyzed posttreatment surveillance imaging strategies and how recurrences were detected.ObjectiveTo refine the understanding of HPV-positive OPSCC recurrence and inform optimal imaging surveillance strategies.Design, Setting, and ParticipantsThis retrospective cohort study involved electronic medical record review at a tertiary care hospital. Patients treated for OPSCC from 2012 to 2019 were extracted, and patients diagnosed with HPV-positive OPSCC were identified. Data were analyzed from December 2022 to May 2023.Main Outcome MeasuresPercentage of patients with a true recurrence, location of recurrence, time of recurrence detection, and method of recurrence detection. Recurrence was demonstrated with a scan after an imaging-established disease-free state 3 to 6 months posttreatment.ResultsOf the 367 patients with HPV-positive OPSCC (mean [SD] age, 60.6 [9.2] years; 310 [84.5%] male), 37 (10.1%) experienced true disease recurrence. Median (IQR) follow-up time of the cohort was 3.6 years (8.5-88 months), defined as time from diagnosis to death or last contact. Within the true recurrence cohort, 21 patients (56.8%) experienced local, regional, or local and regional recurrence (LRR); 15 (40.5%) experienced distant metastasis (DM); and 1 (2.7%) experienced both LRR and DM. The mean (SD) time for detecting LRR was 2.46 (1.94) years and was considerably longer compared to the 1.89 (0.87) years for detecting DM (difference, 0.57 [95% CI, −0.29 to 1.02] years). The majority of patients identified their recurrence through symptom changes (31 [81.1%]) rather than through surveillance imaging (3 [8.1%]).Conclusion and RelevanceIn this cohort study, 10.1% of patients experienced true HPV-positive OPSCC disease recurrence, with most incidences of DM occurring in the lung and brain. Disease recurrence was identified primarily through symptomatic change, suggesting that further research may be needed to understand the optimal surveillance strategies after definitive treatment.
Transcriptome‐Optimized Hydrogel Design of a Stem Cell Niche for Enhanced Tendon Regeneration
Authors: Zhang Wanqi , Rao Ying , Wong Shing Hei , Wu Yeung , Zhang Yuanhao , Yang Rui , Tsui Stephen Kwok‐Wing , Ker Dai Fei Elmer , Mao Chuanbin , Frith Jessica E. , Cao Qin , Tuan Rocky S. , Wang Dan Michelle 
Published: October 2024

AbstractBioactive hydrogels have emerged as promising artificial niches for enhancing stem cell‐mediated tendon repair. However, a substantial knowledge gap remains regarding the optimal combination of niche features for targeted cellular responses, which often leads to lengthy development cycles and uncontrolled healing outcomes. To address this critical gap, an innovative, data‐driven materiomics strategy is developed. This approach is based on in‐house RNA‐seq data that integrates bioinformatics and mathematical modeling, which is a significant departure from traditional trial‐and‐error methods. It aims to provide both mechanistic insights and quantitative assessments and predictions of the tenogenic effects of adipose‐derived stem cells induced by systematically modulated features of a tendon‐mimetic hydrogel (TenoGel). The knowledge generated has enabled a rational approach for TenoGel design, addressing key considerations, such as tendon extracellular matrix concentration, uniaxial tensile loading, and in vitro pre‐conditioning duration. Remarkably, our optimized TenoGel demonstrated robust tenogenesis in vitro and facilitated tendon regeneration while preventing undesired ectopic ossification in a rat tendon injury model. These findings shed light on the importance of tailoring hydrogel features for efficient tendon repair. They also highlight the tremendous potential of the innovative materiomics strategy as a powerful predictive and assessment tool in biomaterial development for regenerative medicine.
Unlocking Intracellular Protein Delivery by Harnessing Polymersomes Synthesized at Microliter Volumes using Photo‐PISA
Authors: Thanapongpibul Chalaisorn , Rifaie‐Graham Omar , Ojansivu Miina , Najer Adrian , Kim Hyemin , Bakker Saskia E. , Chami Mohamed , Peeler David J. , Liu Chenchen , Yeow Jonathan , Stevens Molly M. 
Published: October 2024

AbstractEfficient delivery of therapeutic proteins and vaccine antigens to intracellular targets is challenging due to generally poor cell membrane permeation and endolysosomal entrapment causing degradation. Herein, these challenges are addressed by developing an oxygen‐tolerant photoinitiated polymerization‐induced self‐assembly (Photo‐PISA) process, allowing for the microliter‐scale (10 µL) synthesis of protein‐loaded polymersomes directly in 1536‐well plates. High‐resolution techniques capable of analysis at a single particle level are employed to analyze protein encapsulation and release mechanisms. Using confocal microscopy and super‐resolution stochastic optical reconstruction microscopy (STORM) imaging, their ability to deliver proteins into the cytosol following endosomal escape is subsequently visualized. Lastly, the adaptability of these polymersomes is exploited to encapsulate and deliver a prototype vaccine antigen, demonstrating its ability to activate antigen‐presenting cells and support antigen cross‐presentation for applications in subunit vaccines and cancer immunotherapy. This combination of ultralow volume synthesis and efficient intracellular delivery holds significant promise for unlocking the high throughput screening of a broad range of otherwise cost‐prohibitive or early‐stage therapeutic protein and vaccine antigen candidates that can be difficult to obtain in large quantities. The versatility of this platform for rapid screening of intracellular protein delivery can result in significant advancements across the fields of nanomedicine and biomedical engineering.