bluuebunny/crossref_metadata_2025_split huggingface dataset:
VGLUT2 may improve cognitive function in depressed rats by protecting prefrontal cortex neurons
Authors: Liu Longfei , Hu Yongxue , Shan Qing , Li Peifan , Ma Tianpei , Wang Yiming 
Published: September 2024

ObjectiveDepression may be accompanied by cognitive impairment, but its pathogenesis remains unclear. This study aims to investigate the protective effects of fluoxetine on behavioral performance and prefrontal cortex neuronal damage in rats with depression-associated cognitive impairment, based on the observation of VGLUT2 protein expression.MethodsForty-five SPF-grade male SD rats were randomly divided into three groups (n = 15): normal control group (CON), depression group (DD), and fluoxetine group (DD + F). The CON group was reared normally, while the DD and DD + F groups underwent chronic unpredictable mild stress (CUMS) combined with social isolation to induce a depression-related cognitive dysfunction model. After modeling, the DD + F group was treated with fluoxetine (10 mg/kg, ig) for 14 days. Behavioral tests were performed to assess changes in mood, cognition, learning, and social abilities. Histopathological observations were made to examine pathological changes, neuronal apoptosis, ultrastructure, and dendritic spine density in the prefrontal cortex. The concentration, relative expression level, and mRNA expression of VGLUT2 protein were also measured. Finally, a correlation analysis was performed between the relative expression level and mRNA expression of VGLUT2 protein and the pathological changes in neurons.ResultsCompared to the CON group, the DD group exhibited decreased body weight, anhedonia, increased behavioral despair, reduced locomotor activity and spontaneous exploratory behavior, impaired spatial learning and memory, and decreased social interaction and social cognitive ability. Pathological damage was observed in the prefrontal cortex, with neuronal apoptosis, ultrastructural damage, and reduced neuroplasticity. The concentration, relative expression, and mRNA expression levels of VGLUT2 protein were decreased. Following fluoxetine intervention, the above behavioral phenotypes improved; pathological damage showed varying degrees of recovery; and the concentration, relative expression, and mRNA expression levels of VGLUT2 protein increased. Finally, there was a significant correlation between VGLUT2 protein expression and pathological changes in the prefrontal cortex.ConclusionAfter 28 days of CUMS combined with isolation rearing, rats exhibited impairments in mood, cognition, learning, and social abilities, with neuronal damage and decreased VGLUT2 protein levels in the prefrontal cortex. Following fluoxetine intervention, VGLUT2 protein expression increased, neuronal repair in the prefrontal cortex occurred, depressive-like behavior improved, and cognitive learning and social abilities were restored.
The frontal association area: exercise-induced brain plasticity in children and adolescents and implications for cognitive intervention practice
Authors: Zhang Ziyun , Shi Peng , Zhang Kai , Li Chenyang , Feng Xiaosu 
Published: September 2024

ObjectiveExplore the plasticity of the frontal associative areas in children and adolescents induced by exercise and potential moderating variables.MethodsComputer searches of CNKI, WOS, PubMed and EBSCO databases were conducted, and statistical analyses were performed based on SPSS 25.0, Stata 12.0 and Ginger ALE 2.3 software after literature screening, data extraction and quality assessment were performed independently by two researchers.ResultsA total of 13 articles, including 425 participants aged 8.9∼16.8 years, were included. Frequency analysis revealed that exercise induced enhanced activation in frontal, parietal, occipital, limbic system and cerebellum (P &lt; 0.01). Activation Likelihood Estimation (ALE) meta-analysis revealed that exercise altered the activation status of the frontal association (medial frontal gyrus, middle frontal gyrus, inferior frontal gyrus and precentral gyrus), cuneus, lingual gyrus, cingulate gyrus, parahippocampal gyrus, caudate nucleus and cerebellar apex, with the volume of activation in the frontal association accounting for 61.81% of the total activation cluster volume and an enhanced activation effect. Additionally, the study design, age, gender, nationality, cognitive tasks, as well as exercise intensity, intervention time, and type of exercise may be potential moderating variables. Particularly, sustained exercise induced a decrease in activation in the left parahippocampal gyrus, culmen, and lingual gyrus, while variable exercise induced an increase in activation in the left middle frontal gyrus.ConclusionExercise-induced activation increase in the frontal associative areas of children and adolescents is dominant, especially longer periods of moderate-intensity variable exercise can induce more brain region activation. However, some of the included studies are cross-sectional, and the accuracy of the results still requires further verification.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier PROSPERO, CRD42022348781.
PCAF acetylates AIB1 to form a transcriptional coactivator complex to promote glycolysis in endometrial cancer
Authors: Wu Di , Li Mingxia , Wang Mingyang , Yan Zhifeng , Meng Yuanguang 
Published: September 2024

IntroductionDespite rapid advances in molecular biology, personalized molecular therapy remains a clinical challenge for endometrial cancer due to its complex and heterogeneous tumor microenvironment.Based on clinical findings, AIB1 is a marker molecule for poor prognosis in endometrial cancer and may serve as a potential therapeutic target. Moreover, it is well known that aerobic glycolysis plays an important role in tumour energy metabolism. It has been previously reported in various hormone-related tumour studies that AIB1 affects glycolysis and promotes tumour development. However, the link between AIB1 and aerobic glycolysis in estrogen-dependent endometrial cancer remains unclear.MethodsWe used two endometrial cancer cell lines to validate the high expression of target genes and the effect on the proliferative and invasive capacity of the tumours and verified the pattern of interactions and epigenetic modifications by CHIP and CO-IP techniques. Finally, the conclusions were validated on homozygous miceResultsIn this study, we investigated the transcriptional co-activation functions of AIB1, including its acetylation by PCAF, binding to the c-myc transcription factor, and recruitment of glycolysis-related gene promoters.DiscussionOur findings provide new clues that perturbation of normal homeostatic levels of AIB1 is linked with endometrial cancer. These findings suggest that targeting AIB1-mediated regulation of aerobic glycolysis may offer a novel therapeutic approach for endometrial cancer with high AIB1 expression, opening new avenues for personalized diagnostics and treatment strategies in this disease.
Case report: Two cases of prostate adenocarcinoma progressing to rare sarcomatoid carcinoma with normal PSA levels following endocrine therapy
Authors: Dai Zhicheng , Wang Weikang , Guan Haifang , Wang Xiaohui , Ren Yongheng , Qiu Ying , Liu Jie 
Published: September 2024

BackgroundPatients with prostate adenocarcinoma undergoing regular endocrine therapy may maintain normal PSA levels during follow-up, yet still progress to the highly malignant and rare prostatic sarcomatoid carcinoma, which is seldom reported. This article presents two case studies of prostatic sarcomatoid carcinoma. To date, only a few publications have described prostatic sarcomatoid carcinoma, and the clinical, morphological, and molecular dimensions of prostate adenocarcinoma warrant further investigation.Case descriptionPatient A was admitted two years ago due to difficulty urinating, with a PSA level of 6.35 ng/ml. A prostate needle biopsy was performed, and the postoperative pathology diagnosed prostate adenocarcinoma with a Gleason score of 9 (5 + 4, grade group 5). Citing personal reasons, the patient declined a radical prostatectomy and instead received ongoing androgen deprivation therapy (ADT), comprising goserelin, abiraterone, and prednisone. During follow-up, regular PSA tests showed no abnormalities. One year ago, the patient was admitted again due to difficulty urinating and hematuria, choosing to address only the urethral obstruction. Transurethral resection of the prostate was performed, and the postoperative pathology diagnosed sarcomatoid carcinoma of the prostate. Patient B was admitted three years ago due to difficulty urinating accompanied by hematuria. A prostate MRI and a whole-body radionuclide bone scan suggested prostate cancer with bone metastasis. Prostate needle biopsy confirmed the diagnosis. The patient was then regularly treated with androgen deprivation therapy, using goserelin. Throughout the follow-up period, the PSA levels consistently remained within normal limits. One year ago, the patient was admitted due to rectal bleeding. It was speculated that the symptoms of rectal bleeding might have been caused by the prostate cancer invading the rectal wall. A prostate needle biopsy was performed, and the pathology diagnosed sarcomatoid carcinoma of the prostate.ConclusionsThis case underscores the inadequacy of relying solely on PSA levels to monitor high-grade prostate adenocarcinoma during endocrine therapy, as patients may progress to highly malignant atypical variants despite normal PSA levels. We propose that for high-grade prostate cancer patients who are unable to undergo radical prostatectomy, regular and frequent MRI screenings or repeat biopsies should be integral during endocrine therapy and follow-up. Furthermore, a detailed review of the patient’s treatment history and clinical data, including immunohistochemical findings, might offer deeper clinical insights into prostatic sarcomatoid carcinoma.
Does practice make perfect? Functional connectivity of the salience network and somatosensory network predicts response to mind–body treatments for fibromyalgia
Authors: Medina Sonia , O’Daly Owen , Howard Matthew A. , Feliu-Soler Albert , Luciano Juan V. 
Published: September 2024

BackgroundMind–body treatments can improve coping mechanisms to deal with pain, improve the quality of life of patients with fibromyalgia syndrome (FMS), and reduce perceived pain in some cases. However, responses to these treatments are highly variable, the mechanisms underpinning them remain unclear, and reliable predictors of treatment response are lacking. We employed resting-state blood oxygen level-dependent (rsBOLD) functional magnetic resonance imaging (fMRI) to examine changes in brain functional connectivity (FC) following mind–body treatment that may relate to and predict pain relief.MethodsWe recruited patients with FMS who underwent either mindfulness-based stress reduction (MBSR; n = 18) or a psychoeducational program (FibroQoL; n = 22) and a treatment-as-usual FMS group (TAU; n = 18). We collected rsBOLD data, alongside subjective pain, anxiety, depression, and catastrophizing measures prior to and following treatments. We examined behavioral changes and FC changes in the salience network (SN) and sensorimotor network (SMN) and performed regression analyses to identify predictors for treatment response.ResultsThe MBSR and FibroQoL groups experienced significant reductions in pain catastrophizing. After treatment, the FC of the sensorimotor cortex with the rest of the SMN became significantly reduced in the MBSR group compared to the TAU group. The FC between the SN and the SMN at baseline was negatively correlated with pain reductions following MBSR but positively correlated with pain reductions in the FibroQoL group. These results yielded large to very large effect sizes. Following MBSR, only for those patients with lower baseline SMN-SN FC, minutes of mindfulness practice were positively associated with clinical improvement (small to medium effect size).ConclusionsDifferent mind–body treatments are underpinned by discrete brain networks. Measures of the functional interplay between SN and SMN have the potential as predictors of mind–body treatment response in patients with FMS.
Anal canal duplication with heterotopic gastric mucosa and anal stenosis: first case report and literature review
Authors: Liu Chen , Xu Chuanzhen , Xu Xiaoliang , Zhang Yan , Geng Lei , Mei Yanhui , Ji Hong , Fu Tingliang , Ding Guojian 
Published: September 2024

IntroductionAnal canal duplication (ACD) is a rare entity of gastrointestinal duplication that may be asymptomatic or present complications, such as abscess, fistulae, or malignant changes. The diagnosis and rational management of ACD still need to be clarified.Case presentationWe present a case of an 18-month-old girl with intractable perianal erosion and painful bowel movements for one year, and chronic constipation for six months. Fistulography revealed a tubular structure (3 cm in length), located posterior to the native anal canal. Mucosectomy was performed through a perineal approach combined with a coccigeal approach, and the postoperative course was uneventful. The pathological findings confirmed the diagnosis of ACD with heterotopic gastric mucosa, a rare combination that has not been described in the literature before. A literature search was conducted on the Medline database for studies reporting ACD in children. The study pool consisted of 77 cases of ACD from 32 studies, including the present case. According to our case report and in line with the literature, 43 cases (55.84%) were incidentally found; the most frequent symptom was constipation (14.29%), followed by painful anal mass or sacral pain (10.39%), and recurrent fistula (7.79%). Coexisting diseases were observed in 31 patients (40.26%), including 19 (24.68%) cases associated with presacral masses. Surgical management was employed in 73 patients (94.81%). ACD excision was performed in 47 patients (64.38%), combined with presacral mass resection or coccygectomy in 19 cases (26.03%).ConclusionPreoperative imaging, including fistulography, ultrasonography, and magnetic resonance imaging, can provide useful information, especially for screening its associated anomalies. To prevent potential complications, surgical removal of ACD and associated anomalies is recommended. Mucosectomy may be one of the most effective surgical options for ACD due to its excellent functional outcome.
Analysis of motor behavior in piano performance from the mixed methods approach
Authors: Santisteban Isabel E. , Anguera M. Teresa , Granda-Vera Juan , Pastrana-Brincones José Luis 
Published: September 2024

IntroductionThe focus of this study centers on the extraction, analysis, and interpretation of the motor behavior of advanced-level pianists using observational methodology, itself framed within the field of mixed methods, paying particular attention to those aspects that characterize the pressed and struck touch. The aim of this research was to analyze the motor interactions of activation or inhibition associated with the production of a type of touch in the movements of the right upper limb of the participating pianists.MethodsAn ad hoc observational instrument was built that was incorporated into the software Lince Plus for data recording and coding. Data reliability was guaranteed applying Cohen’s Kappa coefficient, and an analysis of polar coordinates was carried out to identify the motor interactions involved in piano playing.ResultsThe study provided significant information about the interaction of motor functions linked to two types of touch, such as those that occur in the sliding finger movement over the key in the pressed touch or the lifting finger movement above the key in the struck touch, obtaining clearly identified patterns of piano touch motor behavior.DiscussionThis research represents an innovative perspective of the study of piano-playing movement via the direct and perceptible observation of the pianist’s motor behavior in an everyday context. Observational methodology is distinguished by its low degree of internal control, which makes it possible to scientifically study the spontaneous behavior of pianists in their natural environment. This model allows us to describe and analyze piano touch for its application in the field of piano performance and teaching, emphasizing the practical implications of motor interactions in piano touch.
Evaluating the protective effects of mouthguards with neutralizing agents against chlorinated water-induced enamel erosion
Authors: Kitsahawong Kemporn , Pitiphat Waranuch , Thongpaiboon Pawin , Thongpaiboon Sasitorn , Saengsuwannarot Sutthiphon 
Published: September 2024

IntroductionDental erosion is a common problem among swimmers. This study evaluated the effects of mouthguard use with or without neutralizing agents, compared to no mouthguard use, on the microhardness of dental enamel after a swimming simulation.MethodsNinety-six human premolars were randomly allocated into six groups of 16 each: Group A (no mouthguard), Group B (mouthguard only), Group C (mouthguard with fluoride toothpaste), Group D (mouthguard with fluoride-free toothpaste), Group E (mouthguard with CPP-ACP), and Group F (mouthguard with arginine-fluoride toothpaste). Enamel slabs were fixed in a wax model (Typodont Articulator) and used to fabricate mouthguards for all groups except Group A. Each specimen underwent cyclic immersion: 2 h in acidic chlorinated water (pH 3.1) followed by 22 h in artificial saliva, for 28 days, to simulate swimming exposure. The change in enamel surface hardness was measured using a Vickers hardness tester. All groups underwent microhardness testing, scanning electron microscopy, and polarized light microscopy.ResultsThe enamel hardness significantly decreased in all groups after the swimming simulation (paired t-test, P-values &lt; 0.001), except for Group F, which used a mouthguard with arginine-fluoride toothpaste [mean reduction: 17.9 kg/mm2, 95% confidence interval (CI): −1.9, 37.7, P-value = 0.07]. Group A, without a mouthguard, exhibited the highest reduction in enamel surface hardness (mean: 190.6 kg/mm2; 95%CI: 177.4, 203.9), significantly differing from all other groups with mouthguards (P-values &lt; 0.001). However, no statistically significant differences were observed in enamel hardness reduction among the mouthguard groups. SEM micrographs illustrated rough, irregular erosion patterns and several deep porous areas on enamel surfaces of Group A. In contrast, all mouthguard groups showed enamel surfaces similar to sound tooth surfaces. A polarized light microscopic study revealed the deepest dark areas on the enamel surface of Group A.ConclusionsMouthguards significantly reduced enamel microhardness loss compared to no mouthguard use. While no significant differences were found among mouthguard groups with or without neutralizing agents, those lined with arginine-fluoride toothpaste showed the least enamel loss, suggesting its potential protective effect. Within the limitations of this in vitro study, further clinical trials are needed to validate these results.
Clinical values of oblique lumbar interbody fusion on the treatment of single-level degenerative lumbar diseases
Authors: Yu Yu 
Published: September 2024

ObjectivesMinimally invasive transforaminal lumbar interbody fusion (Mis-TLIF) and oblique lumbar interbody fusion (OLIF) are increasingly replacing traditional approaches. This study aimed to compare the clinical outcomes of OLIF and Mis-TLIF in treating single-level degenerative lumbar diseases.MethodsPatients with single-level degenerative lumbar diseases underwent either OLIF (30 patients) or Mis-TLIF (30 patients). Surgical data, including operation time, blood loss, postoperative drainage, and postoperative bed rest duration, were collected. Clinical outcomes were assessed using the Oswestry disability index, the visual analog scale scores for low back pain and leg pain, and Japanese Orthopaedic Association scores for daily ability, along with monitoring of complications.ResultsThe OLIF group showed significantly shorter operative times, less blood loss, reduced postoperative drainage, and shorter bed rest durations than the Mis-TLIF group. At the 1-month follow-up, OLIF patients also demonstrated significantly better clinical outcome scores than Mis-TLIF patients. No significant differences were observed between OLIF and Mis-TLIF patients before surgery and after 3 months. Furthermore, lumbar lordosis and disc height were significantly greater in the OLIF group at the final follow-up.ConclusionsBoth OLIF and Mis-TLIF achieved satisfactory and effective long-term clinical outcomes for single-level lumbar degenerative diseases. However, OLIF resulted in less tissue damage, reduced bleeding, better short-term clinical outcomes, and improved recovery of segmental lordosis compared to Mis-TLIF. Therefore, OLIF appears to be the preferable option over Mis-TLIF.
Influence of the fusion state of particles during slat formation on an oxidized substrate
Authors: Hadjeri Amel , Zirari Mounir , Tahar Souad , Rebhi Redha , Kezrane Mouhamed , Boukraa Moustafa 
Published: September 2024

Achieving a good coating through thermal spray process involves understanding the mechanisms connected to the slat formation. The main objective of this paper is to analyze how the substrate is affected by the thickness of the oxide layer. The next thing to think about is how melting aluminum particles will react with an oxidized substrate. To this end, the fluid structure interaction technique (IFS), the finite element method and the volume of fluid method (VOF) are used to simulate the deposition of particles (molten or semi-molten) onto the part to be covered. The findings reveal that the percentage of the fusion state present in the particle acts directly on the morphology of the splat.
The Productivity Spectrum: An Individual-Level Examination of Servers’ Reactions to Workload and Overwork
Authors: Ashkanani Ahmad M. 
Published: September 2024

Purpose: This study explores the diverse reactions of human servers to variations in workload and overwork, focusing on how these stressors influence service time in a call center setting.Study design/methodology/approach: OLS regressions with fixed effects and clustered standard errors were used to analyze the impact of workload and overwork on service time at both aggregate and individual levels.Sample and data: This research utilizes archival data from a call center at a large Kuwaiti private hospital, covering 58,460 calls handled by 23 agents during October 2016.Results: At the aggregate level, workload has a negative effect on service time, indicating a general speedup response, while overwork shows no significant effect. At the individual level, responses vary significantly among agents, with some agents speeding up, others slowing down, and some demonstrating non-linear responses to workload. Overwork responses also vary, with some agents slowing down, others speeding up, and some showing flat responses.Originality/value: This study contributes to the behavioral queuing literature by emphasizing the importance of individual-level analysis in understanding server responses to workload and overwork. The findings reveal that aggregate analyses can mask significant variations in individual behaviors, crucial for optimizing call center performance. By exposing the spectrum of individual reactions, the study provides deeper theoretical and practical insights into the complex dynamics of service time under different work stressors.Research limitations/implications: The findings provide insights for optimizing call center performance, but further research is needed to validate and generalize these findings across different settings and call center environments.