bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends in Food and Beverage Consumption Among Infants and Toddlers: 2005–2012
Authors: Miles Gandarvaka , Siega-Riz Anna Maria 
Published: May 2017

BACKGROUND:
Nutritional guidance for infants and toddlers is lacking, and the diets of American children in the first 2 years of life are not well characterized.

METHODS:
Cross-sectional data from the NHANES were used to describe the diets of 0- to 23-month-olds in the United States. Participants with complete dietary data were eligible for the analysis (N = 2359). Linear regression models were constructed to identify changes from 2005 to 2008 and from 2009 to 2012 in food and beverage consumption, both overall and within sociodemographic groups.

RESULTS:
We observed several trends toward meeting early-feeding recommendations, such as a decline in the prevalence of complementary feeding among 0- to 5-month-olds. However, the prevalence of vegetable consumption was consistently lower than desired (∼25% of 6- to 11-month-olds and 20% of 12- to 23-month-olds had no reported vegetable consumption on dietary recall days in the 2009–2012 set). Subgroup analyses revealed that some trends were limited to certain populations (eg, a decline in juice consumption was observed among 6- to 11-month-old non-Hispanic whites and non-Hispanic blacks but not among Mexican Americans), and additional trends emerged within groups (eg, the prevalence of breast milk consumption declined among 0- to 5-month-old Mexican Americans).

CONCLUSIONS:
Although there have been some improvements in the diets of 0- to 23-month-olds in recent years, there are areas in which this population continues to fall short of current recommendations. This underscores the need for additional policy guidance for providers and education for parents and caretakers on helping infants and toddlers achieve healthy diets.
A comparison of clinical outcomes of LVEF ≤35% versus LVEF>35% in off-pump coronary artery bypass graft surgery
Authors: Gupta Manju , Mishra Pankaj Kumar , Shoeb Mohd , Agarwal Amit , Prasad Jagdish 
Published: May 2017

Background: Management of patients with coronary artery disease (CAD) and low ejection fraction (EF) is challenging. The objective was to compare the effect of low EF to normal EF on clinical outcomes after off-pump coronary artery bypass graft (OPCABG) surgery.Methods: All patients who underwent CABG at VMMC and Safdarjung hospital, from June 2014 till December 2016 with LVEF≤35% (low EF) as well as LVEF>35% (high EF) were studied and their surgical results analyzed by collecting data retrospectively. Out of 203 total patients the LVEF was less than or equal to 35% in 41 (20.19 %) patients, more than 35% in162 (79.81 %) patients.Results: In LVEF≤35% there were 9 females and the mean age was 60.39±8.76 years, The mean ICU stay in hospital was of 8.73 days, mean ventilator support was 4.01 days, ionotropic support was 4.34 days, mean stay in hospital was of 28.54 days, hospital mortality was 8 (19.51%) in LVEF≤35 versus mean ICU stay in hospital was of 8.73 days, ventilator support was 4.01 days, ionotropic support 4.34 days, mean stay in hospital was of 20.33 days, hospital mortality was 13 (8.02%) in LVEF>35.These data make a survival rate of 80.49% for EF in LVEF≤35 versus 91.98% for EF in LVEF>35.Conclusions: CABG can be safely performed in low-EF patients with minimal postoperative morbidity and mortality in addition to encouraging home discharge rates.
Biofilm Formation of Pseudomonas geniculata (Wright, 1895) Chester, 1901 on Three Fungals Species: Relationship with Incubation Time and Fungal Diameter Size
Authors: Siradje Andi Lindhemuthianingrum , D. Prijambada Irfan , Retnaningrum Endah 
Published: May 2017

<p><em>Pseudomonas geniculata</em> has been isolated from uncontaminated vertisol in Kulon Progo district. The isolate is hydrocarbonoclastic bacterium capable of forming biofilm on the fungal hyphae. Sinergy of both microbe in the form fungal-bacteria biofilm produce high ability to degrading hydrocarbon and survive in its pollution environment. The purpose of this research was to evaluate ability of <em>Pseudomonas</em><em> geniculata</em> (Wright, 1895) Chester, 1901 to form biofilm and its attachment on three fungals species such as <em>Penicillium</em> sp., <em>Penicillium funiculosum</em> and <em>Penicillium crustosum</em>. The diameter size of fungal hyphae was of 1.3 µm, 1.9 µm and 2.4 µm, respectively. <em>P. geniculata</em> required at least 48 h to form biofilms on <em>Penicillium </em>sp. hyphae when incubated in mineral Bushnell Haas Medium suplemented with 2 % glucose at room temperature, with maximal biofilm formation being evident at 360 h. Biofilm attachment on <em>Penicillium </em>sp. hyphae was disrupted by the vortex power of 5 rpm for 20 s. Interaction of <em>P. geniculata</em> and <em>Penicillium </em>sp. that has a smallest diameter size of hypha were more successful on biofilm formation and attachment which could contribute to bacterial survival in environmental stresses. </p><div><p class="Els-keywords"><strong>Keyword:</strong> Biofilm, fungal hyphae<em>, Pseudomonas geniculata</em><em> </em>(Wright, 1895) Chester, 1901, <em>Penicillium</em>. </p></div>
Isolation of Flower Development Regulator Gene SEPALLATA 1 in Phalaenopsis amabilis (L.) Blume
Authors: Febriani Ayu Linda , Anggraini Riska , Naila Mazieda Muthia , Saptasari Murni , Listyorini Dwi , Fatma Wardani Fitri 
Published: May 2017

<p class="Els-Abstract-text"><em>Phalaenopsis amabilis </em>(L.) Blume<em> </em>is an indigenous orchid species in Indonesia. This orchid has a white large flower. The large flower is caused by the existence of gene that has an important role in flower development. One of the genes is <em>SEPALLATA 1. </em>This gene is a member of superfamily MADS-Box gene. <em>SEPALLATA 1 </em>gene is a marker of primordial flower organ. This study aimed to isolate <em>SEPALLATA1</em><em> </em>gene from <em>Phalaenopsis amabilis </em>(L.) Blume by PCR using forward primer 5’-GCT-GGA-GCG-GAT-CGA-GAA-CA-3’and reverse primer 5’-TCA-TGC-AAG-CCA-ACC-AGG-TG-3’<em>. </em>This study successfully amplified 691 bp lengths of <em>SEPPALATA1</em> fragment, lacking 20 bp upstream which consist its start codon.</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong> Flower development regulation, <em>Phalaenopsis amabilis</em> (L.) Blume, SEPALLATA 1 gene.</p></div>
The Role of Ficus crassiramea (Miq.) Miq. for Hornbill Conservation in Borneo Fragmented Tropical Rainforest
Authors: . Budiman , Wijayanti Arini , Lumaby Rusman 
Published: May 2017

<p class="Els-Abstract-text">Fig plants (<em>Ficus</em> spp.) are considered as a key candidate to wildlife conservation in tropical rainforest for its potency on their microhabitat and food supply throughout the years. In this research, we focused only on the <em>Ficus crassiramea</em> (Miq.) Miq. To shows its role for hornbill conservation, we observed the different species of hornbill which visiting the trees and also we observed time, duration, frequency and activity of visits of the hornbill. For the purpose, four trees of <em>F.</em> <em>crassiramea</em> had been observed continuously for 6 d from 06.00 am to 05.00 pm in two fragmented tropical rainforest located in Penajam Paser Utara Regency, Borneo, Indonesia. The result showed, there were three species of hornbill which are visiting the plants in fruiting phase, namely <em>Anthrococeros malayanus</em> (Raffles, 1822), <em>Anthrococeros albi</em><em>r</em><em>ostris</em> (Shaw &amp; Nodder, 1807), and <em>Buceros rhinoceros</em> (Linnaeus 1758) while fig tree in non-fruiting phase is only visiting by <em>A</em><em>.</em><em> malayanus</em> and <em>A. albi</em><em>r</em><em>ostris</em>. There is difference between <em>A</em><em>.</em><em> malayanus</em>, <em>A. albi</em><em>r</em><em>ostris</em> and <em>B</em><em>.</em><em> rhinoceros</em> in time, duration and frequency of visits on <em>F. crassiramea</em>. The most dominant activity of the third hornbill on <em>F.</em> <em>crassiramea</em> in fruiting phase are foraging rather than perched like on non-fruting phase tree. The result convinced the important of <em>F.</em> <em>crassiramea </em>as food source for hornbill conservation.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> <em>F</em><em>icus </em><em>crassiramea</em><em> </em>(Miq.) Miq.; food source; fragmented tropical rainforest; hornbill conservation</p></div>
Traditional Anti Malaria Plants Species of Balikpapan Botanic Garden, East Kalimantan-Indonesia
Authors: Kuswantoro Farid 
Published: May 2017

<p class="Els-Abstract-text">Numerous Kalimantan plants species were traditionaly utilized for its medicinal properties. Ex situ conservation of these species in Balikpapan Botanic Garden (BBG), one of the mostly recent launched botanic garden in Indonesia however, should be appreciated and encouraged to preserved the unique traditional plant-based medicine knowledge of Kalimatan people. An etnobotany literature study was conducted to documented traditional medicine plant species collected in BBG used by indigenous people of Kalimantan to treat malaria. This study found nine plant species collected in BBG namely <em>Aleurites mollucanus </em>(L.) Willd<em>., Alstonia angustiloba </em>Miq.<em>, Alstonia scholaris </em>L. R. Br<em>., Areca catechu </em>L.<em>, Eurycoma longifolia </em>Jack<em>, Lansium parasiticum </em>(Osbeck) K. C. Sahni &amp; Bennet, <em>Morinda</em> <em>citrifolia</em> L., <em>Peronema</em> <em>canescens</em> Jack and<em> Vitex</em> <em>pinnata</em> L. were traditionally used to treat malaria. Further literature study showed that some of these species exhibits biological activity toward <em>Plasmodium berghei </em>and<em> P. falciparum.</em> Three species of this list were classified as rare medical plants in Indonesia. However, further study will still be needed to fully confirm anti malaria activity of these plant species and its potential to become new anti-malaria medicine.</p><div><p class="Els-keywords"><em> </em></p></div><strong>Keywords:</strong> Balikpapan Botanic Garden; ex situ conservation; Malaria; traditional medicine
Skeletal Malformation on Balb-C Foetal Mice (Mus musculus) Administered by Immature Pineapple Fruits Extract
Authors: Prasetyawati Fila , Gofur Abdul , Lestari Umie 
Published: May 2017

<p class="Els-Abstract-text">Nutrients for pregnant woman affect the development of foetus in the womb. Pineapple has bromelain enzyme which benefit in medicinal uses, but it can be considered as teratogenic agents due to its proteolytic activities. The period of organogenesis is the critical period in foetal development. Examination of foetal anatomy is still a fundamental part of teratological studies despites of modern molecular techniques. Molecular techniques can be done by conducting the skeletal proteome analysis to evaluate the teratogenic effects on the foetal skeleton. This research was performed to examine the effect of immature pineapple fruits extract on the occurrence of skeletal malformation and identify the protein profiles of skeleton on Balb-C foetal mice. Pregnant mice were administered by immature pineapple fruits extract orally by gavage with doses 0 %, 20 %, 40 %, 60 % and 80 % at day 6 d to 15 d of gestation. Skeletal malformation observed after Alizarin red-Alcian blue staining, and SDS-PAGE was conducted to identify the protein profiles of foetal skeleton. Immature pineapple fruits extract caused the decreasing of weight and length of litter, causing hemorrhage, ossification retardation on sternebra, metacarpal, metatarsal, caudal vertebrae, undulated costae and asymmetric sternebra. Protein profiling analysis showed that skeletal proteins at molecular weight of 32 kDa, 35 kDa, 42 kDa and 49 kDa did not found in foetal mice administered by immature pineapple fruits extract.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> bromelain; immature pineapple fruits; skeletal malformation; skeletal proteome analysis.</p></div>
Shelf Life of Artocarpus lowii King’s Seeds And Its Viability
Authors: Damayanti Frisca , Fatma Wardani Fitri 
Published: May 2017

<p class="Els-Abstract-text"><em>Artocarpus lowii</em> King is a species of Moraceae family members. This species is widely utilized by people as food, medicine, and timber. <em>A. lowii</em> is propagated by seed. <em>A. lowii</em> seeds belong to the recalcitrant seeds, which have a short shelf life. Therefore, it is necessary to find the right storage method for storing <em>A. lowii</em> seeds, in order that <em>A. lowii</em> seed has longer shelf life. The purpose of this study was to determine the viability <em>A. lowii</em> with storage time treatments and find the efficient storage method. <em>A. lowii</em> seeds were stored by covering the seeds with mosses in a jar. It was taken 40 seeds every week for viability test. The 30 seeds have planted in the sand media and 10 seeds for grain moisture content calculations. Results of this study were water content of <em>A. lowii</em> seed on 0 wk to 7 wk increased, and the viability of <em>A. lowii</em> seeds have still high enough to 9 wk around 60 %. Therefore, seed storage methods using mosses can be used as an alternative to extend the shelf life of <em>A. lowii</em> seed.</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong><em> Artocarpus lowii</em> King; recalcitrant seed; storage media</p></div>
Geometry Morphometry and Health Status of Hawksbill Turtle (Eretmochelys imbricata Linnaeus, 1766) in Maratua Island, East Kalimantan-Indonesia
Authors: Maulida Firly Fathiya , Hadia Susilo , Imron Muhammad Ali , Reischig Thomas 
Published: May 2017

<p class="Els-Abstract-text">Research on hawksbill turtles in the Maratua Island is still very few. Meanwhile, population of hawksbills was believed to decline due to human exploitation. The aim of this research was to study the morphology of hawksbill through geometric morphometric approach and health status which includes the existence of epibionts, mechanical damages and Body Condition Index. The survey was conducted from 5 to 18 September 2014. The method used was the hand˗catching by snorkeling and diving for catching turtles, morphometric measurements and documentation of the turtle body parts using the camera. Morphological aspects were analyzed with linear regression and geometry approach with Microsoft Excel and ImageJ software. Health status was analyzed with observation of epibionts and mechanical damages through photos and calculation of Body Condition Index. There were 11 turtles that have been caught. The results indicated the growth of straight carapace length notch to tip affect the growth of the other body parts of hawksbill turtle and based approach to geometry, shape growth of hawksbill turtles that are found in Maratua not changed but only have added size. Based on the health status through observation epibionts, mechanical damages and analysis of Body Condition Index, hawksbill in Maratua still relatively healthy compared with studies elsewhere.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> <em>Eretmochelys imbricata</em> (Linnaeus, 1766); geometry; Maratua; morphometry</p></div>
Potential Lutein Extract of Broccoli (Brassica oleracea L. var. italica) as Antiradical Abts (2,2 - Azinobis Acid, 3-Ethyl Benzothiazoline-6-Sulfonic Acid)
Authors: . Kusmiati , Sri Agustini Ni Wayan 
Published: May 2017

<p class="Els-Abstract-text">Broccoli (<em>Brassica oleracea</em> L. var. italica) is one vegetable that is often consumed by people with high nutrient content i.e protein, fat, vitamin C, fiber, potassium and calcium. Carotenoids and glutathione are contained in broccoli that are antioxidants. Lutein is a carotenoid group contained in broccoli is capable of reducing the accumulated products of free radicals in the body. The purpose of this research is to extract and identify compounds lutein from flowers of broccoli and test its potential as antiradical to the compound ABTS (2,2-Azinobis acid, 3-ethyl benzatiazolin-6-Sulfonat). Identification of lutein compounds qualitatively by TLC showed Rf value of 0.72 cm. Analysis of Lutein by HPLC showed a peak at the retention time of 3:03 min, approaching retention time of lutein standard 3:01 min. Test of potency of antiradical ABTS showed that lutein extract from flower of broccoli has antioxidant activity with IC<sub>50</sub> value of 157.527 8 µg · mL<sup>–1</sup>. Fractionation of lutein extract using column chromatography with Silica gel 60 as stationary phase and mixture of methanol : chloroform : n-hexane (4:20:8) as mobile phase resulted in 4 fractions with antioxidant activity increased. Fraction 3 has the highest antiradical ABTS activity with IC<sub>50</sub> 39.584 2 µg · mL<sup>–1</sup>. Vitamin E is used as a standard had antiradical ABTS activity IC<sub>50</sub> 5.303 6 µg · mL<sup>–1</sup>.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> ABTS ; anti-radical; Broccoli (<em>Brassica oleracea </em>L. var<em>.</em><em> </em>italica); lutein.</p></div>
Isolation 3’-end Fragment of Pun1 gene from Capsicum frutescens L. cultivar Cakra Hijau
Authors: Juliandri Ria Reinnata , Zairina Shelly , Khasna Elhah Nailul , . Dahlia , Listyorini Dwi 
Published: May 2017

<p><em>Pun1 </em>gene <a href="file:///C:/Users/Mohamad%20Mostafa/Desktop/Knowledge%20E/In%20Press%20Conferences/ICBS-2015/Source%20files/ICBS-2015/Soure%20papers-ICBS-2015/25.%20Ria%20Reinnata-080117_Roy.doc#_msocom_1">[A1]</a> is the one of candidate gene that responsible to determine pungency in Capsicum. In previous researches, 1 310 bp fragment of 1 671 bp <em>Pun1 </em>gene from<em> Capsicum frutescens </em>L. cv. Cakra Hijau had been isolated. The purpose of this research was to isolate of 3’-<em>end</em> fragment and get full length of <em>Pun1</em> gene from <em>C. frutescens</em> L. cv. Cakra Hijau. DNA isolation was done using modified procedure. The method used to isolate the gene was PCR with a pair of primer, <em>forward primer </em>5’-GAA-GGT-GGC-AGA-AGA-ATC-AG-3’ and <em>reverse primer</em> 5’-TTG- TTG ACC-GTA-AAC-TTC-CG- 3’. The result successfully to get 715 bp length DNA fragment. The assembly of this fragment into previous research produced a full length of 1 671 bp <em>Pun1</em> gene from <em>C. frutescens</em> L. cv. Cakra Hijau consist of 738 bp first exon fragment, 348 bp intron fragment, and 585 bp second exon fragment.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> C. frustescens L. cv. Cakra Hijau; gene isolation; <em>Pun1</em> gene.</p></div>
The Phylogenetic Study of the White-Bellied Sea Eagle [Haliaeetus leucogaster (Gmelin, 1788)] Based on DNA Barcoding Cytochrome-c Oxidase Subunit I (COI)
Authors: Retnaningtyas Riri Wiyanti , Hermadhiyanti Windri , Listyorini Dwi 
Published: May 2017

<p class="Els-Abstract-text">Even though not yet considered as endangered, White-bellied Sea Eagle’s global population is decreasing due to illegal hunting, bird trading, and deforestation. So far, there hasn’t been any report regarding the phylogenetic study of the White-bellied Sea Eagle inhabiting the coastal regions of Java. Moreover, there hasn’t been any report on the genetic data, especially COI gene, of the White-bellied Sea Eagle living in coastal area of Java. Thus, in this research, two individuals of <em>Heliaeetus leucogaster</em><em> </em>(<a title="Johann Friedrich Gmelin" href="https://en.wikipedia.org/wiki/Johann_Friedrich_Gmelin">Gmelin</a>, 1788); <em> </em>are compared based on its COI gene sequence to the member of genus <em>Haliaeetu</em>s to determine their position in the phylogenetic tree of genus Haliaeetus. COI gene amplification is performed using <em>Forward</em> primer BirdF1 5’- TTC TCC AAC CAC AAA GAC ATT GGC AC-3’ and <em>Reverse </em>primer BirdR2 5’ ACT ACA TGT GAG ATG ATT CCG AAT-3’. The phylogenetic analysis using MEGA6 with <em>Maximum Likelihood </em>method shows that <em>Haliaeetus leucogaster</em> in this study is related to <em>Haliaeetus leucocephalus</em> (Linnaeus, 1766), <em>Haliaeetus pelagicus</em> (Pallas, 1811), and <em>Haliaeetus albicilla</em> (Linnaeus, 1758).</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong> phylogenetic study; <em>Heliaeetus leucogaster</em><em> </em>(<a title="Johann Friedrich Gmelin" href="https://en.wikipedia.org/wiki/Johann_Friedrich_Gmelin">Gmelin</a>, 1788); DNA barcoding, <em>C</em><em>ytochrome-c </em><em>O</em><em>xidase </em><em>S</em><em>ubunit</em> I (COI).</p></div>
Salinity Stress Alters Nutrient Uptake and Causes the Damage of Root and Leaf Anatomy in Maize
Authors: Hasana Rusdi , Miyake Hiroshi 
Published: May 2017

<p class="Els-Abstract-text">Salinity is one of major problems in agriculture especially in arid and semiarid area due to causes the damage of many aspects in plant growth and development. This study observed root and leaf anatomy and nutrient uptake in maize plants exposed to salinity stress. Maize seedlings were placed in the plantation room under same temperature, humidity and light intensity conditions and were treated with 0 %, 1 %, 2 % and 3 % NaCl for 5 d. Anatomy of root and leaf were observed using scanning electron microscopy (SEM). Nutrient uptake was estimated by the content of trace elements of leaves. Trace element were quantified using inductively coupled plasma-mass spectrometry (ICP-MS), but chlorine was determined by an atomic absorption flame spectrometer. The results showed that salinity slightly damaged roots anatomy. Epidermis cells and parenchyma cells of cortex and pith were shrinkage in 2 % and 3 % NaCl-treated plants. Leaf anatomy showed mesophyll and bundle sheath cells which slightly suppressed. Meanwhile, chloroplasts content inside those cells were dramatically decreased. Anatomical damage of roots and leaves was accompanied by altering uptake of some trace elements. The contents of aluminum, calcium, iron, magnesium, sodium, chlorine, in NaCl-treated plants were higher than control. Otherwise, boron, potassium and phosphor were lower in NaCl-reated plants. The rest of trace elements were in comparable concentration.</p><p> </p><p><strong>Keywords:</strong> maize; leaf; nutrient; root; salinity</p><div><p class="Els-keywords"> </p></div>
Biological Network Analysis of Genes Involve in Embryogenesis of Oil Palm using ClueGO
Authors: Sudania Widyartini Made , Achmad Tanjung Zulfikar , Toruan-Mathius Nurita , Liwang Tony 
Published: May 2017

<p class="Els-Abstract-text">The application of DNA sequencing technologies has a major impact on molecular biology, especially in understanding genes interaction in a certain condition. Due to a large number of genes produced by this high-throughput technology, a proper analysis tool is needed for data interpretation. ClueGO is a bioinformatics tool, an easy to use Cytoscape plug-in that strongly improves biological function interpretation of genes. It analyzes a cluster or comparing two clusters and comprehensively visualizes their group functions. This tool is applied to identify biological networks of genes involved in embryogenesis of oil palm, the most critical phase in oil palm tissue culture process. Two ESTs sequencing data from the GenBank database under accession number EY396120-EY413718 and DW247764-DW248770 were used in this study. Fifty-two and one hundred eight groups of genes were identified using biological process in Gene Ontology setting from the database of EY396120-EY413718 and DW247764-DW248770, respectively. Thirty-one groups of genes were consistently occurred in both ESTs. According to the literature, these genes play an important role in cell formations and developments, stresses and stimulus responses, photosynthesis and metabolic processes that indicate the involvement of these groups of genes in oil palm embryogenesis processes. ClueGO is the appropriate tool to analyze a large data set of genes in a specific condition, such as embryogenesis of oil palm.</p><div><p class="Els-keywords"><em> </em></p></div><strong>Keywords:</strong> callus embryogenesis; Cytoscape plug-in; DNA sequencing; expressed sequence tag; KEGG pathways