bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vibrational biospectroscopy characterizes biochemical differences between cell types used for toxicological investigations and identifies alterations induced by environmental contaminants
Authors: Heys Kelly A. , Shore Richard F. , Pereira M. Glória , Martin Francis L. 
Published: June 2017

Abstract

The use of cell-based assays is essential in reducing the number of vertebrates used in the investigation of chemical toxicities and in regulatory toxicology assessment. An important factor in obtaining meaningful results that can be accurately extrapolated is the use of biologically appropriate cell lines. In the present preliminary study, attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopy with multivariate analysis was used to assess the fundamental biomolecular differences between a commonly used cell line, MCF-7 cells, and an environmentally relevant cell line derived from mallard (Anas platyrhynchos) dermal fibroblasts. To better understand differences in basic cell biochemistry, the cells were analyzed in the untreated state or post exposure to polychlorinated biphenyl (PCB) and polybrominated diphenyl ester (PBDE) congeners. The main spectral peaks in spectra from both cell types were associated with cellular macromolecules, particularly proteins and lipids, but the spectra also revealed some cell-specific differences. Spectra from untreated mallard fibroblasts spectra contained a large peak associated with lipids. The cell-related differences in lipids and deoxyribonucleic acid (DNA) were also identified as regions of spectral alteration induced by PBDE and PCB exposure. Although lipid alterations were observed in post treatment spectra from both cell types, these may be of more significance to mallard fibroblasts, which may be the result of increased intracellular lipid as determined by Nile red staining. Untreated MCF-7 cell spectra contained unique peaks related to DNA and nucleic acids. The DNA-associated spectral regions were also identified as areas of considerable alteration in MCF-7 cells exposed to some congeners including PBDE 47 and PCB 153. The findings indicate that in their native state, MCF-7 and mallard cells have unique biochemical differences, which can be identified using ATR–FTIR spectroscopy. Such differences in biochemical composition may influence cell susceptibility to environmental contaminants and therefore influence the choice of cell type used in toxicology experiments. To our knowledge, the present study is the first study to analyze the biochemistry of a mallard dermal fibroblast cell line and to use ATR–FTIR spectroscopy for this purpose. Thus ATR–FTIR spectroscopy is demonstrated to be a useful tool for exploration of biomolecular variation at the cellular level and with further development, it could be used as part of a panel of cell-based assays to indicate when different results might be seen in environmental species compared with currently used cell lines. Environ Toxicol Chem 2017;36:3127–3137. © 2017 SETAC
Spatial and temporal trends of poly- and perfluoroalkyl substances in fish fillets and water collected from pool 2 of the Upper Mississippi River
Authors: Newsted John L. , Holem Ryan , Hohenstein Gary , Lange Cleston , Ellefson Mark , Reagen William , Wolf Susan 
Published: June 2017

Abstract

In 2011, poly- and perfluoroalkyl substances (PFASs) were analyzed in surface water and fish fillet samples taken from Pool 2 of the Upper Mississippi River, a 33-mile stretch inclusive of the Minneapolis/St. Paul, Minnesota (USA) metropolitan area. Approximately 100 each of bluegill, freshwater drum, smallmouth bass, and white bass were sampled within the study area. Surface water samples were also collected from each of the 10 sampling reaches established for the study. Water and fillet samples were analyzed for perfluorinated carboxylic acids (C4–C12), perfluorinated sulfonic acids (C4, C6, and C8), and perfluorooctane sulfonamide. Perfluorooctane sulfonate (PFOS) was observed with the greatest frequency in fish fillets and ranged from 3.0 to 760 ng/g wet weight. Mean (geometric) PFOS concentrations in bluegill, freshwater drum, smallmouth bass, and white bass were 20, 28, 29, and 58 ng/g wet weight, respectively. When compared with fish data collected in 2009, a significant reduction (p &lt; 0.05) in PFOS concentrations was noted. This finding was confirmed based on data from studies conducted in 2012 and 2013. Overall, between 2009 and 2013, PFOS concentrations decreased by 65, 76, and 50% for bluegill, freshwater drum, and white bass, respectively (44% decrease for smallmouth bass from 2009 to 2012). These declines in fish PFOS concentrations are consistent with ongoing efforts to effectively control sources of PFASs to the Mississippi River. Environ Toxicol Chem 2017;36:3138–3147. © 2017 SETAC
Geology, geochemistry, and genesis of the Dashui Carlin‐type gold deposit in the West Qinling orogenic belt, Gansu Province, China
Authors: Peng Xiuhong , Yang Hai , Zhang Jiangsu 
Published: June 2017

The Dashui gold deposit occurs in the south of West Qinling orogenic belt where is belonged to a famous gold triangle area named “Shan‐Gan‐Chuan” in Northwest China. The presence of native Au associated with hematite alteration is unique in China. The ore bodies at Dashui mainly occur in the limestone and dolomitic limestone units within the Lower Triassic Maresongduo Formation. The granodioritic dykes that intrude the Triassic sedimentary rocks are locally mineralized because of later carbonate alteration. The ore bodies are vein, lenticular, cystic, and dendritic in shape and are controlled by NW‐, NNE‐ (near N‐S‐), and E‐W‐trending faults. Gold mineralization occurs commonly at fault intersections and contact zones between the carbonate and magmatic rocks. The NNE‐ (near N‐S‐) trending faults and associated pinnate fractures are most favorable loci for gold deposition. The metallic minerals include native Au, pyrite, cinnabar, and marcasite, which are mostly overprinted by an oxidized mineral assemblage of hematite and limonite. Gold mineralization is closely associated with decalcification, silicification, and carbonate alterations. Gold content in altered samples correlates with Si enrichment and Ca and Mg depletions. Rare earth elements (REE) increase with Al2O3 and Fe2O3 in altered carbonate rocks, indicating that an enrichment of REE may be closely related to argillic alteration and supergene weathering. The REE patterns of altered carbonate rocks are more likely consistent with granodiorite instead of the ones of fresh carbonate rocks. Moreover, δ34S values of Dashui deposit ranging from −1.8‰ to 4.1‰ indicate that the deep‐sourced magmatic hydrothermal fluids may have worked during the early stage mineralization. Altered carbonate rocks show higher δ13C and lower δ18O values than those of the original host rock. Therefore, the REE, S, C, and O data suggest that the ore‐forming fluids may be magmatic derived and later interact and exchange with host rock and meteoric water. The Dashui gold deposit is characterized by structural and stratigraphic controls; Au–As–Sb–Hg geochemical association; alteration of decalcification, silicification, and argillization; low base‐metals; high Au/Ag ratio; and low δ18O values. These features are consistent with Carlin‐type Au deposits in Western USA, suggesting that the Dashui deposit is a Carlin‐type deposit that formed in the Middle Jurassic to Early Cretaceous (170 to 135 Ma).
Natural gas characteristics, fluid evolution, and gas charging time of the Ordovician reservoirs in the Shuntuoguole region, Tarim Basin, NW China
Authors: Ni Zhi‐Yong , Wang Tie‐Guan , Li Mei‐Jun , Chen Zhong‐Hong , Ou Guang‐Xi , Cao Zi‐Cheng 
Published: June 2017

The Shuntuoguole region is situated at the center of the Tarim Basin, in NW China. It is bordered by the Tazhong Uplift to the west and the Manjiaer Depression to the east. Data obtained on samples collected from exploration wells highlight the large potential for gas discoveries in this region. Component and stable carbon isotopic analyses have been performed on gas samples from 5 wells in the Shuntuoguole region. The dryness coefficients of gas samples vary from 0.95 to 1.00, indicating that the gas samples belong to overmatured dry gas. The carbon isotopic values (δ13C) of all gas samples are heavier than −40‰, compared to the typical gas samples with different sources in the Tarim Basin, revealing that the gas in the Shuntuoguole region originated from source rocks in the Cambrian. The SN‐1 exploitation well was selected to undertake fluid inclusion studies revealing the mechanisms of natural gas charging and fluid evolution. Data from the SN‐1 well drill core indicate a 1,700‐ to 7,050‐m‐thick sedimentary sequence containing Ordovician to Tertiary strata. The Middle Ordovician Yijianfang Formation (Fm) constitutes one of the most important gas reservoirs. The Yijianfang Fm consists principally of limestone containing widely distributed sparry calcite. Numerous fluid inclusions are observed in calcite, and 4 types of fluid inclusions are recognized based on the observed phases and components. These are pure CH4(P‐type), CH4‐bearing (C‐type), aqueous (W‐type), and solid‐bearing (S‐type) inclusions. The trapping temperatures and pressures, determined through the intersection of isochores from coeval fluid inclusions, reach 183 °C to 216 °C and 1,914 bars to 2,196 bars, respectively. When taking into account the regional burial history, we find the natural gas charged during the Neogene interval, from 20 Ma to 3 Ma.
A service recovery method to enhance customer satisfaction with a case study of motion sport game
Authors: Chiu Ming‐Chuan , Chang Yi‐Jie 
Published: June 2017

AbstractService recovery is a concept that involves regaining customer satisfaction and loyalty. Although existing studies have addressed its importance, few service recovery methodologies have been developed. Thus, there is a need to develop a systematic framework that can first detect customer status and then provide appropriate recovery recommendations to ensure customer satisfaction. This study develops such a framework in relation to an individual health maintenance practice. While regular moderate exercise is known to promote good health and help prevent disease, the lack of exercise remains a crucial health issue. This study presents a logistic regression model that uses both physiological data (heart rate and blood pressure) and psychological data (ratings of perceived exertion) to detect the effectiveness of an individual's exercise and to provide sports‐related service recovery suggestions as needed. To implement and assess this service recovery model, the use of a motion gaming system is proposed.
Precise spike timing dynamics of hippocampal place cell activity sensitive to cholinergic disruption
Authors: Newman Ehren L. , Venditto Sarah Jo C. , Climer Jason R. , Petter Elijah A. , Gillet Shea N. , Levy Sam 
Published: June 2017

AbstractNew memory formation depends on both the hippocampus and modulatory effects of acetylcholine. The mechanism by which acetylcholine levels in the hippocampus enable new encoding remains poorly understood. Here, we tested the hypothesis that cholinergic modulation supports memory formation by leading to structured spike timing in the hippocampus. Specifically, we tested if phase precession in dorsal CA1 was reduced under the influence of a systemic cholinergic antagonist. Unit and field potential were recorded from the dorsal CA1 of rats as they completed laps on a circular track for food rewards before and during the influence of the systemically administered acetylcholine muscarinic receptor antagonist scopolamine. We found that scopolamine significantly reduced phase precession of spiking relative to the field theta, and that this was due to a decrease in the frequency of the spiking rhythmicity. We also found that the correlation between position and theta phase was significantly reduced. This effect was not due to changes in spatial tuning as tuning remained stable for those cells analyzed. Similarly, it was not due to changes in lap‐to‐lap reliability of spiking onset or offset relative to either position or phase as the reliability did not decrease following scopolamine administration. These findings support the hypothesis that memory impairments that follow muscarinic blockade are the result of degraded spike timing in the hippocampus.
Pharmacokinetic study of bortezomib administered intravenously in Taiwanese patients with multiple myeloma
Authors: Huang Shang‐Yi , Chang Cheng‐Shyong , Liu Ta‐Chih , Wang Po‐Nan , Yeh Su‐Peng , Ho Ching‐Liang , Kuo Ming‐Chung , Lin Hsuan‐Yu , de Jong Jan , Chen Jia‐Yi , Yang Ya‐Wen 
Published: June 2017

AbstractThis phase 4, single‐arm, non‐randomized, open‐label, post approval commitment study evaluated the pharmacokinetics and safety of bortezomib in Taiwanese patients with multiple myeloma. Patients (≥20 years) with measurable secretory multiple myeloma (serum monoclonal IgG ≥10, IgA/IgE ≥5, IgD ≥0.5 g/L, IgM present [regardless of level], and urine M protein of ≥200 mg/24 h) received intravenous bortezomib 1.3 mg/m2, twice weekly for 2 weeks, followed by a 10‐day resting phase (days 12 to 21). Pharmacokinetics and safety were assessed at pre‐specified time points. All enrolled patients (n = 18, men: 11; women: 7) completed the study. Mean (SD) Cmax (maximum observed plasma concentration) on day 11 was 266 (77.5) ng/mL, approximately 60% higher compared with non‐Asian patients receiving a similar bortezomib regimen but with overlapping ranges. Because of the protracted terminal phase, half‐life (t1/2), area under the plasma concentration‐time curve from time 0 to infinity (AUC∞), volume of distribution (Vz), and systemic clearance were not assessable. All patients experienced treatment‐emergent adverse events (TEAEs); 78% were drug‐related. Most commonly reported TEAEs were thrombocytopenia (n = 11 [61%]), neutropenia (n = 9 [50%]), leukopenia (n = 6 [33%]), and diarrhoea (n = 6 [33%]); the most common serious adverse event was pneumonia (n = 2 [11%]). One patient had a dose reduction due to a TEAE of thrombocytopenia. Overall, bortezomib exposure (AUC) in Taiwanese patients (AUClast [SD]: 230 [147] ng·h/mL) with twice weekly intravenous administration was comparable with non‐Asian population (AUClast [SD]: 241 [82] ng·h/mL). Bortezomib treatment was associated with manageable toxicity profile and did not limit the continuity of therapy.
The contextual‐level effects of social trust on health in transitional countries: Instrumental variable analysis of 26 countries
Authors: Habibov Nazim , Cheung Alex 
Published: June 2017

SummaryWe analyse the effect of contextual‐level social capital on health status in a sample of 26 transitional countries of Central and South Europe, Mongolia, and the former Soviet Union for 2006‐2010 (N = 51 911). Contextual‐level social capital is conceptualized as country‐level social trust, while health status is conceptualized as self‐rated health. We use ordinary least squares and instrumental variable regressions to address endogeneity and especially to rule out reverse causality. Both instrumental variable and ordinary least squares regressions suggest a strong positive effect of country‐level trust on health. This finding is consistent for the whole sample as well as separate regional estimations.
Hyperthermic intraperitoneal chemoperfusion as a component of multimodality therapy for ovarian and primary peritoneal cancer
Authors: Magge Deepa , Ramalingam Lekshmi , Shuai Yongli , Edwards Robert P. , Pingpank James F. , Ahrendt Steven S. , Holtzman Matthew P. , Zeh Herbert J. , Bartlett David L. , Choudry Haroon A. 
Published: June 2017

Background and ObjectivesThe role of hyperthermic intraperitoneal chemoperfusion (HIPEC) in the multimodality treatment of ovarian peritoneal metastases (OPM) and primary peritoneal cancer (PPC) remains controversial. We hypothesized that cytoreductive surgery (CRS) and HIPEC would provide meaningful survival benefit without excessive morbidity.MethodsWe reviewed clinicopathologic and perioperative data following 96 CRS‐HIPEC procedures for primary or recurrent OPM and PPC. Kaplan‐Meier survival curves and multivariate Cox‐regression models identified prognostic factors affecting oncologic outcomes.ResultsCRS‐HIPEC was mostly performed for recurrent disease (56.3%) and high‐grade serous carcinoma (72.9%). Platinum‐based systemic chemotherapy was administered to 89.5% of patients, with 75.5% having platinum‐sensitive disease at CRS‐HIPEC. Complete macroscopic resection was achieved in 70.8% of patients. Clavien‐Dindo grade 3/4 morbidity occurred in 23.4% of patients; three patients died within 60‐days postoperatively. Median overall survival from diagnosis of peritoneal metastases and CRS‐HIPEC was 78 and 38 months, respectively. Completeness of cytoreduction, pathologic subtype, and 30‐day morbidity were independent predictors of survival in multiple regression analysis.ConclusionsOur study demonstrates promising survival data and supports the role of HIPEC in the multimodality treatment algorithm for primary or recurrent OPM and PPC. However definite indications and timing of HIPEC need to be clarified by prospective studies.
Bone resection for facial cutaneous malignancies
Authors: Brunner Markus , Ch'ng Sydney , Shannon Kerwin , Clifford Anthony , Ashford Bruce , Elliott Michael , Clark Jonathan R. 
Published: June 2017

Background and ObjectivesThe aim of this study is to analyze the clinical outcomes of patients who underwent bone resection for cutaneous malignancy of the face and scalp.MethodsWe retrospectively collected patient data from 62 patients who underwent bone resection for craniofacial cutaneous malignancy of the face and scalp over the last 10 years. We investigated risk factors for disease progression and assessed the utility of pre‐operative imaging to predict bone, dura, and brain infiltration.ResultsOut of all factors analyzed, brain invasion, surgical margin involvement, and dural margin involvement were found to significantly reduce survival. CT and MRI correctly predicted bone infiltration in 88% and 89% of cases. MRI correctly predicted dura invasion in 89% but grossly underestimated the amount of dural invasion in 23% of reports.ConclusionsOur data indicate that the resection of bone is a reasonable surgical option in the treatment of patients with advanced cutaneous malignancies of the face and scalp. Brain invasion and positive margins reduced the probability of survival.
Effect of abdominopelvic sepsis on cancer outcome in patients undergoing sphincter saving surgery for rectal cancer
Authors: Van de putte Dirk , Van Daele Elke , Willaert Wouter , Pattyn Piet , Ceelen Wim , Van Nieuwenhove Yves ,   on behalf of PROCARE
Published: June 2017

BackgroundIn rectal cancer, the significance of abdominopelvic sepsis (APS) on metastatic tumor growth remains uncertain. We aimed to analyze the effect of abdominopelvic sepsis on long‐term survival in patients undergoing restorative rectal cancer surgery.MethodsData were used from the Belgian PROCARE rectal cancer registry. The effect of abdominopelvic infection on survival was assessed in uni‐ and multivariable Cox regression models. The effect of clinical and pathological covariates was controlled by propensity score‐based matching of cases with controls. The effect of abdominopelvic sepsis on the rate of local and metastatic recurrence was evaluated using crosstabulation and the Pearson χ2 test.ResultsIn univariable analysis, the presence of APS was associated with significantly worse overall survival (HR 1.3, P = 0.025). After propensity score matching including age, BMI, tumor level, pTstage, pN stage, CRM, tumor grade, number of lymph nodes, and presence of lymphovascular invasion, the association of APS with OS was no longer significant (HR 1.26, 95%CI 0.92‐1.74, P = 0.15). No differences were observed in the risk of local or metastatic recurrence (3.6% vs 2.9% and 13% vs 16.5%).ConclusionsIn this analysis APS after rectal cancer resection was not significantly associated with OS, metastatic, or local recurrence.
Advanced real‐time multi‐display educational system (ARMES): An innovative real‐time audiovisual mentoring tool for complex robotic surgery
Authors: Lee Joong Ho , Tanaka Eiji , Woo Yanghee , Ali Güner , Son Taeil , Kim Hyoung‐Il , Hyung Woo Jin 
Published: June 2017

IntroductionThe recent scientific and technologic advances have profoundly affected the training of surgeons worldwide. We describe a novel intraoperative real‐time training module, the Advanced Robotic Multi‐display Educational System (ARMES).MethodsWe created a real‐time training module, which can provide a standardized step by step guidance to robotic distal subtotal gastrectomy with D2 lymphadenectomy procedures, ARMES. The short video clips of 20 key steps in the standardized procedure for robotic gastrectomy were created and integrated with TilePro™ software to delivery on da Vinci Surgical Systems (Intuitive Surgical, Sunnyvale, CA).ResultsWe successfully performed the robotic distal subtotal gastrectomy with D2 lymphadenectomy for patient with gastric cancer employing this new teaching method without any transfer errors or system failures. Using this technique, the total operative time was 197 min and blood loss was 50 mL and there were no intra‐ or post‐operative complications.ConclusionsOur innovative real‐time mentoring module, ARMES, enables standardized, systematic guidance during surgical procedures.
Microscopic lymphovascular invasion is an independent predictor of survival in resected pancreatic ductal adenocarcinoma
Authors: Epstein Jeffrey D. , Kozak Geoffrey , Fong Zhi Ven , He Jin , Javed Ammar A. , Joneja Upasana , Jiang Wei , Ferrone Cristina R. , Lillemoe Keith D. , Cameron John L. , Weiss Matthew J. , Lavu Harish , Yeo Charles J. , Fernandez‐del Castillo Carlos , Wolfgang Christopher L. , Winter Jordan M. 
Published: June 2017

Background and Objectives Despite routine inclusion of lymphovascular invasion (LVI) status in pathologic reports of resected pancreatic ductal adenocarcinomas (PDA), the clinical implications of LVI have not been well characterized.Methods This study is a retrospective review of 2640 patients who underwent a pancreatectomy for PDA at Thomas Jefferson University Hospital, Massachusetts General Hospital, or Johns Hopkins Hospital (2003‐2014). Clinical and pathologic records were extracted from institutional databases.Results The median post‐resection survival for the total cohort was 19.2 months with a 5‐year survival rate of 15.2%. In a multivariate Cox proportional hazards model including conventional pathologic features, LVI was an independent predictor of survival (HR = 1.14, P = 0.017). In a stratified Kaplan‐Meier survival analysis, patients with N0, LVI‐ PDA had a significantly improved overall survival compared to those with N0, LVI+ PDA (median 31 vs 24 mo, P = 0.020). Similarly, patients with N1, LVI‐ PDA had superior survival to patients with N1, LVI+ disease (18.6 vs 16.5 mo, P = 0.001).Conclusions As the first large scale study focused on the clinical impact of LVI status in PDA, these data indicate that this routinely reported pathologic feature is a bona fide and independent adverse prognostic factor.