bluuebunny/crossref_metadata_2025_split huggingface dataset:
Olive oil enrichment in phenolic compounds during malaxation in the presence of olive leaves or olive mill wastewater extracts
Authors: Kiritsakis Konstantinos , Rodríguez‐Pérez Celia , Gerasopoulos Dimitrios , Segura‐ Carretero Antonio 
Published: April 2017

A combined study was performed to determine the effect of olive leaf aqueous extract (OLE), olive mill wastewater (OMWW) or water addition during the malaxation step on total phenolic content (TPC), antioxidant activity, virgin olive oil composition and quality parameters of olive oil. For these purposes, the Folin‐Ciocalteu assay, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, and HPLC‐ESI‐TOF/MS were employed. The experiments were carried out at semi‐industrial scale in a cold extraction olive oil mill using olives of Koroneiki cultivar. Olive oil produced by olive paste which was malaxed without adding water, OMWW, or OLE was employed as control. The use of OMWW and OLE allowed obtaining oils with higher significant TPC compared to that malaxed with water (146.2 and 151.8 vs. 132.8 μg Eq GAE/g oil, respectively). The antioxidant activity followed the same trend. The use of OMWW and OLE enabled an oil enrichment in oleuropein derivatives, especially in 3,4‐DHPEA‐EDA. Despite the fact that acidity and oxidation (K270 and K232 values) were higher in the aforementioned oils compared to the oil extracted with water, all values were under the European Union commission parameters for extra virgin olive oil (EVOO) denomination. However, only the oil extracted using OLE presented slightly higher fruity, bitter, and pungent positive attributes compared to the control and to the oil extracted with water, thus, making OLE a promising by‐product for improving olive oils.Practical applications: This research provides new insight for the improvement of olive oil extraction process in order to obtain high‐phenol EVOO which could be useful for the olive oil, nutraceuticals, and cosmeceuticals industries. Besides, this study could serve as a starting point for improving the olive oil production process, while reducing the impact of its by‐products.The addition of Olive mil wastewater (OMWW) and olive leaf extract during malaxation, allowed obtaining enriched oils with a higher content in total phenolics, and antioxidant activity compared to those obtained adding water. These enriched oils presentes higher quantities of oleuropein derivatives and specifically of 3,4‐DHPEA‐EDA.
Motivational and behavioural models of change: A longitudinal analysis of change among men with chronic haemophilia‐related joint pain
Authors: Elander J. , Richardson C. , Morris J. , Robinson G. , Schofield M.B. 
Published: April 2017

AbstractBackgroundMotivational and behavioural models of adjustment to chronic pain make different predictions about change processes, which can be tested in longitudinal analyses.MethodsWe examined changes in motivation, coping and acceptance among 78 men with chronic haemophilia‐related joint pain. Using cross‐lagged regression analyses of changes from baseline to 6 months as predictors of changes from 6 to 12 months, with supplementary structural equation modelling, we tested two models in which motivational changes influence behavioural changes, and one in which behavioural changes influence motivational changes.ResultsChanges in motivation to self‐manage pain influenced later changes in pain coping, consistent with the motivational model of pain self‐management, and also influenced later changes in activity engagement, the behavioural component of pain acceptance. Changes in activity engagement influenced later changes in pain willingness, consistent with the behavioural model of pain acceptance.ConclusionsBased on the findings, a combined model of changes in pain self‐management and acceptance is proposed, which could guide combined interventions based on theories of motivation, coping and acceptance in chronic pain.SignificanceThis study adds longitudinal evidence about sequential change processes; a test of the motivational model of pain self‐management; and tests of behavioural versus motivational models of pain acceptance.
Neural correlates of experimental trauma memory retrieval
Authors: Gvozdanovic Geraldine A. , Stämpfli Philipp , Seifritz Erich , Rasch Björn 
Published: April 2017

AbstractObjectivesTraumatic memories such as intrusions and flashbacks play a major role in the development and maintenance of post‐traumatic stress disorder (PTSD). A thorough understanding of the neural mechanisms underlying traumatic memories is indispensable for precise diagnosis, for personalized treatment and prevention. In particular, the identification of early neural predictor variables for intrusion development shortly after trauma exposure requires detailed investigation. Experimental design: Here, we examined the neural correlates of early experimental trauma memory retrieval in a traumatic film paradigm in 42 young healthy females, using both implicit and explicit retrieval tasks. Principal observations: We show that implicit experimental trauma retrieval specifically involved the retrosplenial cortex and the anterior cingulate cortex (ACC), while both retrieval tasks resulted in trauma‐related activity in the posterior cingulate cortex (PCC) and the precuneus. Importantly, neural activity early after experimental trauma exposure predicted later intrusion development, with independent contributions from activity in the retrosplenial cortex (implicit retrieval) and the PCC (explicit retrieval). Additional analyses revealed a stronger connectivity between the bilateral amygdala and the supplementary motor area, precentral and paracentral lobule for the control group compared to the experimental trauma group. Conclusions: Our study gives new insights in the neural correlates of experimental trauma memory retrieval and their predictive value for subsequent symptom development. Our results could provide the basis for personalized early treatment and prevention of PTSD. Hum Brain Mapp, 2017. © 2017 Wiley Periodicals, Inc. Hum Brain Mapp 38:3592–3602, 2017. © 2017 Wiley Periodicals, Inc.
Surface orientation effects on bending properties of surgical mesh are independent of tensile properties
Authors: Simon David D. , Andrews Sharon M. , Robinson‐Zeigler Rebecca , Valdes Thelma , Woods Terry O. 
Published: April 2017

AbstractIntroductionCurrent mechanical testing of surgical mesh focuses primarily on tensile properties even though implanted devices are not subjected to pure tensile loads. Our objective was to determine the flexural (bending) properties of surgical mesh and determine if they correlate with mesh tensile properties.MethodsThe flexural rigidity values of 11 different surgical mesh designs were determined along three textile directions (machine, cross‐machine, and 45° to machine; n = 5 for each) using ASTM D1388‐14 while tracking surface orientation. Tensile testing was also performed on the same specimens using ASTM D882‐12. Linear regressions were performed to compare mesh flexural rigidity to mesh thickness, areal mass density, filament diameter, ultimate tensile strength, and maximum extension.ResultsOf 33 mesh specimen groups, 30 had significant differences in flexural rigidity values when comparing surface orientations (top and bottom). Flexural rigidity and mesh tensile properties also varied with textile direction (machine and cross‐machine). There was no strong correlation between the flexural and tensile properties, with mesh thickness having the best overall correlation with flexural rigidity.ConclusionsCurrently, surface orientation is not indicated on marketed surgical mesh, and a single mesh may behave differently depending on the direction of loading. The lack of correlation between flexural stiffness and tensile properties indicates the need to examine mesh bending stiffness to provide a more comprehensive understanding of surgical mesh mechanical behaviors. Further investigation is needed to determine if these flexural properties result in the surgical mesh behaving mechanically different depending on implantation direction. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 854–862, 2018.
Connexin43 and Runx2 Interact to Affect Cortical Bone Geometry, Skeletal Development, and Osteoblast and Osteoclast Function
Authors: Buo Atum M , Tomlinson Ryan E , Eidelman Eric R , Chason Max , Stains Joseph P 
Published: April 2017

ABSTRACT The coupling of osteoblasts and osteocytes by connexin43 (Cx43) gap junctions permits the sharing of second messengers that coordinate bone cell function and cortical bone acquisition. However, details of how Cx43 converts shared second messengers into signals that converge onto essential osteogenic processes are incomplete. Here, we use in vitro and in vivo methods to show that Cx43 and Runx2 functionally interact to regulate osteoblast gene expression and proliferation, ultimately affecting cortical bone properties. Using compound hemizygous mice for the Gja1 (Cx43) and Runx2 genes, we observed a skeletal phenotype not visible in wild-type or singly hemizygous animals. Cortical bone analysis by micro–computed tomography (μCT) revealed that 8-week-old male, compound Gja1+/–Runx2+/– mice have a marked increase in cross-sectional area, endosteal and periosteal bone perimeter, and an increase in porosity compared to controls. These compound Gja1+/–Runx2+/– mice closely approximate the cortical bone phenotypes seen in osteoblast-specific Gja1-conditional knockout models. Furthermore, μCT analysis of skulls revealed an altered interparietal bone geometry in compound hemizygotes. Consistent with this finding, Alizarin red/Alcian blue staining of 2-day-old Gja1+/–Runx2+/– neonates showed a hypomorphic interparietal bone, an exacerbation of the open fontanelles, and a further reduction in the hypoplastic clavicles compared to Runx2+/– neonates. Expression of osteoblast genes, including osteocalcin, osterix, periostin, and Hsp47, was markedly reduced in tibial RNA extracts from compound hemizygous mice, and osteoblasts from compound hemizygous mice exhibited increased proliferative capacity. Further, the reduced osteocalcin expression and hyperproliferative nature of osteoblasts from Cx43 deficient mice was rescued by Runx2 expression. In summary, these findings provide evidence that Cx43 and Runx2 functionally intersect in vivo to regulate cortical bone properties and affect osteoblast differentiation and proliferation, and likely contributes to aspects of the skeletal phenotype of Cx43 conditional knockout mice. © 2017 American Society for Bone and Mineral Research
Cytokinetic Abscission: Phosphoinositides and ESCRTs Direct the Final Cut
Authors: Gulluni Federico , Martini Miriam , Hirsch Emilio 
Published: April 2017

ABSTRACTCytokinetic abscission involves the fine and regulated recruitment of membrane remodeling proteins that participate in the abscission of the intracellular bridge that connects the two dividing cells. This essential process is mediated by the concomitant activity of the endosomal sorting complex required for transport (ESCRT) and the vesicular trafficking directed to the midbody. Phosphoinositides (PtdIns), produced at plasma membrane, and endosomes, act as molecular intermediates by recruiting effector proteins involved in multiple cellular processes, such as intracellular signaling, endo‐ and exo‐cytosis, and membrane remodeling events. Emerging evidences suggest that PtdIns have an active role in recruiting key elements that control the stability and the remodeling of the cytoskeleton from the furrow ingression to the abscission, at the end of cytokinesis. Accordingly, a possible concomitant and coordinated activity between PtdIns production and ESCRT machinery assembly could also exist and recent findings are pointing the attention on poorly understood ESCRT subunits potentially able to associate with PtdIns rich membranes. Although further studies are required to link PtdIns to ESCRT machinery during abscission, this might represent a promising field of study. J. Cell. Biochem. 118: 3561–3568, 2017. © 2017 Wiley Periodicals, Inc.
Enhanced Entrapment and Improved in Vitro Controlled Release of N‐Acetyl Cysteine in Hybrid PLGA/Lecithin Nanoparticles Prepared Using a Nanoprecipitation/Self‐Assembly Method
Authors: Ahmaditabar Parvin , Momtazi‐Borojeni Amir A. , Rezayan Ali H. , Mahmoodi Mahboobeh , Sahebkar Amirhossein , Mellat Mostafa 
Published: April 2017

ABSTRACTTo enhance the in vitro controlled release of N‐acetyl cysteine (NAC), hybrid nanoparticles (NPs) consisting of a poly(lactide‐co‐glycolide) (PLGA) hydrophobic core and a soybean lecithin mono‐layer coat were prepared. Hybrid NPs were synthesized using a nanoprecipitation combined with self‐assembly method. To characterize prepared NPs, zeta potential, diameter size, surface morphology, disparity, and lipid coating of hybrid NPs were detrmined using dynamic light scattering, scanning electron microscope and Fourier transform infrared spectroscopy techniques. High‐performance liquid chromatography was employed to evaluate drug loading yield and encapsulation efficiency and in vitro drug release of prepared NPs. The cytotoxicity of hybrid NPs was assayed on normal L929 alveolar epithelial cells using MTT method. Prepared NPs were found to disperse as individual NPs with a well‐defined spherical shape. The hydrodynamic diameter and surface charge of NAC‐loaded hybrid NPs were 81.8 ± 1.3 nm and −33.1 ± 2.1 mV, respectively. Drug loading yield and encapsulation efficiency of NAC‐loaded hybrid NPs were found to be 38 ± 2.1% and 67 ± 5.7%, respectively. Prepared hybrid NPs showed no significant cytotoxicity against normal alveolar cells. Our data suggest that the hybrid PLGA‐lecithin NPs may be An efficient controlled release drug delivery system for NAC. J. Cell. Biochem. 118: 4203–4209, 2017. © 2017 Wiley Periodicals, Inc.
A Self‐Determination Theory Perspective on Postpartum Depressive Symptoms and Early Parenting Behaviors
Authors: Brenning Katrijn , Soenens Bart 
Published: April 2017

ObjectiveOn the basis of self‐determination theory, this study investigates longitudinal associations between satisfaction and frustration mothers’ basic psychological needs (i.e., autonomy, relatedness, competence) and mothers’ postnatal depressive symptoms and early parenting behaviors.MethodParticipants were 214 women assessed during their pregnancy (Time 1), 4 months after delivery (Time 2), and when the child was 2 years old (Time 3).ResultsResults demonstrate effects of prenatal basic psychological needs (needs frustration more specifically) on postnatal depressive symptoms. In addition, general prenatal needs satisfaction and frustration predicted more relationship‐specific needs satisfaction and frustration, respectively (in the mother–child relationship). In turn, postnatal relationship‐specific needs satisfaction predicted more high‐quality parenting when the child is 2 years old. More specifically, needs satisfaction was related to more maternal responsiveness and autonomy support.ConclusionOverall, these findings underscore the importance of psychological needs experiences for both mothers’ personal adjustment after birth and for the early mother–child relationship.
A Comparison of Practitioner and Student Writing in Civil Engineering
Authors: Conrad Susan 
Published: April 2017

AbstractBackgroundNumerous studies have identified a gap between the writing skills of engineering program graduates and the demands of writing in the workplace; however, few studies have analyzed the writing of practitioners and students to better understand that gap and inform teaching materials.PurposeThis study sought to compare word‐level, sentence‐level, and organizational differences in writing by practitioners and students and to identify differences that are important for engineering practice. I also sought to demonstrate the untapped potential for linguistic analyses to contribute to understanding engineering writing.Design/MethodI used techniques from applied linguistics – corpus linguistics and rhetorical move analysis – supplemented with interviews of practitioners and students. The analysis investigated the interaction of language features, their functions, and writers' motivations.ResultsStudent writing had more complicated sentence structures, less accurate word choice, more errors in grammar and punctuation, and less linear organization. These characteristics decreased effectiveness in areas that practitioners considered important: accurate and unambiguous content; fast, predicable reading; liability management; and attention to detail. Underlying the student writing problems were misconceptions about effective writing, ignorance of genre expectations, weak language skills, and a failure to appreciate that written words, not just calculations, express engineering content.ConclusionsThe findings better define the gap between student and practitioner writing, and are a basis for instructional materials that target important student writing weaknesses.
Syntheses, Crystal Structures, and Spectral Characterization of Six Novel Benzimidazolyl Substituted Triaryltriazoles
Authors: Zhou Yong‐Fei , Zhang Shi‐Pei , Feng Zhe , Shen Xuan , Zhu Dun‐Ru 
Published: April 2017

Six new benzimidazolyl substituted triaryltriazoles, 3‐(2‐pyridyl)‐4‐(p‐R‐phenyl)‐5‐(2‐benzimidazolyl)‐1,2,4‐triazoles (L1: R = OCH3; L2: R = CH3; L3: R = H; L4: R = Br; L5: R = Cl; L6: R = F) were successfully synthesized. Yield of L1–6 is in the range from 61 to 76%. The compounds L1–6 were characterized by UV–vis, FTIR, 1H‐NMR, ESI‐MS spectra, and elemental analysis. Additionally, the absolute configurations of L1–5 were determined by single crystal X‐ray crystallography.
Antimicrobial Evaluation of New Quinoxaline Derivatives Synthesized by Selective Coupling with Alkyl Halides and Amino Acids Esters
Authors: Boraei Ahmed T. A. , El Tamany El Sayed H. , Ali Ibrahim A. I. , Gebriel Sara M. 
Published: April 2017

Alkylation of quinoxaline scaffold 1 in the presence of K2CO3 preferred N‐alkylation than O‐alkylation. Quinoxaline hydrazide 6 was successfully coupled with various amino acids, esters, and amines via azide‐coupling method. New heterocyclic compounds containing quinoxaline linked to 1,3,4‐oxadiazolethione or pyrazole were obtained from cyclization of 6 with CS2 and acetylacetone, respectively. A series of hydrazide Schiff's bases were formed from hydrazide 6 by condensation with a set of aldehydes and ketones. NMR spectroscopy and mass spectrometry were used for structure elucidation of new compounds. The antimicrobial activity of the synthesized compounds was investigated toward two wild‐type bacterial strains (Staphylococcus aureus and Escherichia coli) and two fungal species (Alternaria brassicicola and Fusarium oxysporum). Four compounds displayed a significant activity toward S. aureus. The ester 4 showed higher activity than the standard drugs, which make it a promising lead compound.
Prospective comparison of biphasic contrast‐enhanced CT, volume perfusion CT, and 3 Tesla MRI with diffusion‐weighted imaging for insulinoma detection
Authors: Zhu Liang , Xue Huadan , Sun Zhaoyong , Li Ping , Qian Tianyi , Xing Xiaoping , Li Naishi , Zhao Yupei , Wu Wenming , Jin Zhengyu 
Published: April 2017

PurposeTo evaluate the diagnostic performance of biphasic contrast‐enhanced CT (CECT), volume perfusion CT (VPCT) and 3 Tesla MRI with diffusion‐weighted imaging (DWI), in patients with clinically suspected insulinomas.Materials and MethodsThis prospective study was approved by the institutional review board. Sixty‐four patients with clinically suspected insulinomas underwent biphasic CECT, VPCT, and 3T MR with DWI. Two radiologists independently determined the presence/absence of tumor using a 5‐scale confidence level. Conspicuity of the lesion and clarity of tumor‐to‐pancreatic duct distance were graded. Receiver operating characteristic analysis was performed to compare diagnostic performance.ResultsForty‐seven patients were tumor positive, with 51 tumors. The differences between the areas under the curve values for tumor detection were as follows: 0.715 (CECT), 0.903 (VPCT), 0.832 (MRI without DWI) and 0.955 (MRI with DWI) for reader 1, and 0.738 (CECT), 0.895 (VPCT), 0.841 (MRI without DWI), and 0.956 (MRI with DWI) for reader 2. MRI with DWI and VPCT were significantly more accurate than CECT for insulinoma detection (P = 0.01 and 0.02 for reader 1, and P = 0.01 and 0.03 for reader 2). Lesion conspicuity was better on MRI compared with VPCT (P = 0.01), and both were better than CECT (both P < 0.01). Tumor‐to‐pancreatic duct distance was better appreciated on MRI, compared with CECT and VPCT (both P < 0.01). The weighted k values indicate good to excellent agreement between observers for determining tumor presence/absence (k = 0.64–0.84).ConclusionThe 3T MRI with DWI and VPCT are significantly more accurate than CECT for insulinoma detection. MRI demonstrates higher tumor conspicuity and is superior in depicting the tumor‐to‐duct distance.Level of Evidence: 1Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2017;46:1648–1655.
Delayed gadolinium‐enhanced MRI of cartilage (dGEMRIC) and T2 mapping of talar osteochondral lesions: Indicators of clinical outcomes
Authors: Rehnitz Christoph , Kuni Benita , Wuennemann Felix , Chloridis Dimitrios , Kirwadi Anand , Burkholder Iris , Kauczor Hans‐Ulrich , Weber Marc‐André 
Published: April 2017

PurposeTo evaluate the utility of delayed gadolinium‐enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping in evaluation of type II osteochondral lesions (OCLs) of the talus and define cutoff values for identifying patients with good/poor clinical outcomes.Materials and Methods28 patients (mean age, 42.3 years) underwent T2 mapping and dGEMRIC at least 1.5 years (mean duration, 3.5 years) after microfracture (n = 12) or conservative (n = 16) treatment for type II OCL. Clinical outcomes were considered good with an American Orthopedic Foot and Ankle Society score ≥80. The T1/T2‐values and indices of repair tissue (RT; cartilage above the OCL) were compared to those of the adjacent normal cartilage (NC) by region‐of‐interest analysis. The ability of the two methods to discriminate RT from NC was determined by area under the receiver operating characteristics curve (AUC) analysis. The Youden index was maximized for T1/T2 measures for identifying cutoff values indicative of good/poor clinical outcomes.ResultsRepair tissue exhibited lower dGEMRIC values (629.83 vs. 738.51 msec) and higher T2 values (62.07 vs. 40.69 msec) than NC (P < 0.001). T2 mapping exhibited greater AUC than dGEMRIC (0.88 vs. 0.69; P = 0.0398). All T1 measures exhibited higher maximized Youden indices than the corresponding T2 measures. The highest maximized Youden index for T1difference was observed at a cutoff value of 84 msec (sensitivity, 78%; specificity, 83%).ConclusionWhile T2 mapping is superior to dGEMRIC in discriminating RT, the latter better identifies good/poor clinical outcomes in patients with type II talar OCL.Level of Evidence: 2Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2017;46:1601–1610.