bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin
Authors: Akbar Fahmi , Sudrajat Agus Oman , Subaidah Siti 
Published: February 2017

ABSTRACT The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could influence the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp Litopenaeus vannamei sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of L. vannamei shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm. Keyword: PMSG, AD, sperm quality, Litopenaeus vannamei ABSTRAK Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan pregnant mare serum gonadotropin (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname Litopenaeus vannamei. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang L. vannamei. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal. Kata kunci: PMSG, AD, kualitas sperma, Litopenaeus vannamei
Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker
Authors: Widanarni . , Meha D. , Nuryati Sri , Sukenda . , Suwanto A. 
Published: February 2017

<p>Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of <em>Vibrio harveyi</em> in shrimp larvae. <em>V. harveyi</em> M. G<sub>3</sub> and G<sub>7</sub> that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate <em>V. harveyi</em> resistant to rifampicin. Two groups of shrimp post-larvae (PL<sub>5</sub>) were immersed for 30 min in 106 CFU/ml of mutants and wild type of <em>V. harveyi</em>, respectively; and then placed in a 2 liter shrimp rearing tank for five days. A control group was immersed in sterile seawater. Growth curve analysis and pathogenicity assay of <em>V. harveyi </em> showed that each of the <em>V. harveyi</em> mutant exhibited almost identical profiles to that of the wild type parental strain and did not show alteration in their pathogenicity. Sample from dead shrimp larvae showed that the dead shrimp larvae were infected by <em>V. harveyi </em>Rf<sup>R</sup>, indicated that rifampicin-resistant marker effective as a reporter to assay pathogenicity of <em>Vibrio harveyi </em>in shrimp larvae.</p> <p>Key words: shrimp larvae, <em>Vibrio harveyi</em>, rifampicin-resistant, molecular marker</p>
The Effect Hydroquinone Extracted from Sonneratia caseolaris Fruit to Control Vibrio harveyi Artificial Infection on Tiger Shrimp, Penaeus monodon Fab.
Authors: Arifuddin . , Sukenda . , Dana D. 
Published: February 2017

<p>The role of hydroquinone extracted from <em>Soneratia caseolaris </em>fruit to control <em>Vibrio harveyi </em>infection on tiger prawn was carried out. <em>In vitro </em>experiment was conducted using disc diffusion and MIC <em>{minimum inhibitory concentration) </em>methods to know the sensitivity of <em>V. harveyi </em>to hydroquinone. Two kinds of <em>in vivo </em>experiments were (1) hydroquinone was injected into shrimps muscle and a week later the shrimps were challenged with <em>V. harveyi </em>(2) the shrimps were challenged with <em>V. harveyi </em>and one day later hidroquinone was injected. Total count of live <em>V. harveyi </em>on the shrimps and survival rate were observed after challenge test. Hydroquinone showed antibacterial activity with MIC at 3000 ppm. Hydroquinone injected shrimp showed higher survival rate compared with control (100% vs 50%). Total count of <em>V. harveyi </em>from injected shrimp, either before or after challenged, decreased by 2,61xl04 cfu/g and l,61xl04 cfu/g, respectively. These findings indicated that crude hydroquinone have anti-bacterial effect to control <em>V. harveyi </em>infection.</p> <p>Key words: hydroquinone, <em>Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon </em></p> <p> </p> <p>ABSTRAK</p> <p>Telaah peran hidrokuinon yang diekstraksi dari buah <em>Soneratia caseolaris </em>untuk mengontrol infeksi <em>Vibrio harveyi </em>pada udang windu dilakukan. Percobaan <em>in vitro </em>dilakukan menggunakan metode difusi cakram dan MIC <em>{minimum inhibitory concentration) </em>untuk mengetahui sensitivitas <em>V. harveyi </em>terhadap hidrokuinon. Percobaan <em>in vivo </em>dilakukan dengan dua cara (1) hidrokuinon disuntikkan pada otot udang dan seminggu kemudian udang diuji tantang dengan <em>V. harveyi </em>(2) udang ditantang terlebih dahulu dengan <em>V. harveyi </em>dan sehari kemudian hidrokuinon disuntikkan. Jumlah total bakteri <em>V. harveyi </em>hidup pada udang dan kelangsungan hidup udang diamati setelah uji tantang. Hidrokuinon menunjukkan aktivitas antibakteri dengan MIC 3000 ppm. Udang yang diinjeksi dengan hidrokuinon mempunyai kelangsungan hidup yang lebih tinggi dibandingkan kontrol (100% vs 50%). Jumlah total <em>V. harveyi </em>pada udang yang diinfeksi, baik sebelum maupun sesudah, masing-masing turun sampai 2,61 x 104 cfu/g dan 1,61 x 104 cfu/g. Hasil ini menunjukkan bahwa hidrokuinon mempunyai efek anti-bakterial untuk mengontrol infeksi <em>V. harveyi. </em></p> <p>Kata kunci: hidrokuinon, <em>Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon</em></p>
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn.
Authors: S Nuryati , Dana D. , Malole M.B. , Pasaribu F.H. 
Published: February 2017

<p>Primary shrimp cell culture from lymphoid organ of <em>Penaeus monodon </em>was successfully developed. Minced tissues of lymphoid organ were seeded by tripsin enzyme and culture in Leibovit'z-15 supplemented with 10% fetal bovine serum and 5% NaCl. Plates were then incubated at 27 °C with 5% C02 supply. Confluent cells were infected by white spot baculovirus (WSBV). Cytopathic effect (CPE) induced by white spot baculovirus showed characteristics of detachment cells and lysis forming giant polycaryon cells. Primary shrimp cell culture could be continued by anvanced cell culture resulting cell line could be used virus media.</p> <p>Key words : cell culture, lymphoid organ, tiger shrimp, invitro, virus</p> <p> </p> <p>ABSTRAK</p> <p>Kultur sel primer dari organ limfoid udang windu <em>Penaaeus monodon </em>telah berhasil dilakukan. Potongan organ limfoid dipisahkan menjadi sel-sel tunggal dengan menggunakan enzim tripsin. Sel-sel tunggal tersebut dikuitur dalam media Leibovit'z-15 dengan tambahan 10 % fetal bovine serum dan 5 % NaCl. Biakan diinkubasi pada suhu 27 °C dengan kandungan C02 sebanyak 5 %. Biakan yang telah konfluen diinfeksi dengan virus WSBV dan menghasilkan <em>cytopathic effect </em>(CPE) dengan karakter sel lepas dari perlekatan dan mengalami lisis yang menyisakan <em>giant cells </em>dengan inti banyak <em>(polycaryons). </em>Kultur sel primer ini dapat ditindaklanjuti dengan kultur sel dengan pasase yang berulang-ulang sehingga dapat menghasilkan sel lestari <em>(cell line) </em>untuk media tumbuh virus.</p> Kata kunci: biakan sel, organ limfoid, udang windu, invitro, virus
Effect of Temperature on Reproduction and Sex Ratio of Guppy (Poecilia reticulata Peters)
Authors: Arfah H. , Mariam S. , Alimuddin . 
Published: February 2017

<p>Water temperature could affect the reproduction of broodstock and sex ratio of progeny. In this study, broodstock of guppy (<em>Poecilia reticulata</em> Peters) was reared in different temperature to determine its effect on reproduction of broodstock and sex ratio of their progeny. The result of study show that broodstock reared at 27°C produced more fry (16 males mean) than that of 30°C (10 males), while broodstock reared at 33°C produced no progeny. Percentage of male fish produced by broodstock reared at 30°C is higher than that of 27°C. Incubation time of embryo before birth is sorter in broodstock reared at 30°C (4-12 days) compared with 27°C (18-22 days). However, several progeny of broodstock reared at 30°C had abnormal vertebrae.</p> <p>Keywords: guppy, <em>Poecilia reticulata</em>, sex reversal, reproduction, monosex</p> <p> </p> <p>ABSTRAK</p> <p>Suhu air inkubasi diduga dapat mempengaruhi reproduksi induk ikan dan nisbah kelamin keturunannya. Pada penelitian ini, induk ikan gapi (<em>Poecilia reticulata</em> Peters) dipelihara pada suhu 27°C, 30°C dan suhu 33°C untuk mengetahui pengaruhnya terhadap reproduksi dan rasio kelamin keturunannya. Hasil penelitian menunjukkan bahwa induk ikan gapi yang dipelihara pada suhu 27°C menghasilkan anak lebih banyak (rata-rata 16 ekor) daripada di suhu 30°C (10 ekor), sementara induk gagal melahirkan pada suhu pemeliharaan 33°C. Proporsi anak jantan yang dihasilkan oleh induk yang dipelihara pada suhu 30°C lebih banyak dibandingkan pada suhu 27°C. Waktu inkubasi embrio sebelum dilahirkan oleh induk yang dipelihara pada suhu 30°C lebih singkat, yaitu 4-12 hari, dibandingkan pada suhu 27°C, 18-22 hari. Namun demikian beberapa anak ikan yang lahir dari induk yang dipelihara pada suhu suhu 30°C mengalami abnormalitas pada bagian tulang belakangnya. </p> <p>Kata kunci: ikan gapi, <em>Poecilia reticulata</em>, <em>sex reversal</em>, reproduksi, monoseks</p>
Effect of Orally Administrated Acriflavine to larval of Red Tilapia (Oreochromis sp.) on Their Sex Ratio
Authors: Junior M. Zairin , Nurlestiyoningrum D. , Raswin M. 
Published: February 2017

<p>Uncontrolled and excessive spawning of red tilapia (<em>Oreochromis</em> sp.) hinders fish growth to reach marketable size. Several techniques have been developed to produce monosex population to overcome the problem, such as sex reversal by hormonal administration. Androgen hormone is generally employed to meet the goal, although the use of this hormone had been restricted and its price is relatively high. Acriflavine is an alternative chemical and its effect on sex development toward male fish has been proved. In this study, acriflavine at dose of 0, 15, 25 and 35 mg/kg feed were fed to 10-day-old larvae for 6 weeks. Feeding of larvae by diet containing 17a-methyltestosterone (MT) at dose of 50 mg/kg was also observed as a comparison of acriflavin treatment. The results indicated that feeding of larvae by diet containing acriflavine 25 mg/kg (78.3%) and 35 mg/kg (79.0%) produced significantly higher percentage of male fish compared to 15 mg/kg and control. However, the result is still lower compared to that of MT treatment (99.2%). Acriflavine treatment had no effect on survival of red tilapia and survival rate was similar among treatments, ranged from 91.7% to 95.0%.</p> <p>Keywords: red tilapia, <em>Orechromis</em> sp., monosex, acriflavine</p> <p> </p> <p>ABSTRAK</p> <p>Pemijahan tak terkontrol dan berlebih pada ikan nila merah (<em>Oreochromis </em>sp.) menghambat pertumbuhan ikan untuk mencapai ukuran konsumsi. Beberapa teknik yang telah dikembangkan untuk menghasilkan ikan monosek sebagai solusi permasalahan tersebut antara lain dengan seks reversal menggunakan hormon. Secara umum, hormon golongan androgen digunakan untuk mencapai tujuan, namun penggunaannya mulai dibatasi dan harganya relatif mahal. Akriflavin merupakan bahan alternatif yang terbukti dapat mempengaruhi perkembangan kelamin ke arah jantan. Pada penelitian ini dilakukan pemberian akriflavin dengan dosis 0, 15, 25 dan 35 mg/kg pakan ke larva umur 10 hari selama 6 minggu. Sebagai pembanding juga dilakukan pemberian pakan yang mengandung hormon 17a-metiltestosteron dosis 50 mg/kg pakan. Hasil penelitian menunjukkan bahwa pemberian pakan yang mengandung akriflavin 25 mg/kg pakan (78,3%) dan 35 mg/kg pakan (79,0%) menghasilkan ikan jantan dengan persentase lebih banyak dibandingkan dengan perlakuan 15 mg/kg (72,46%) dan kontrol (58,37%). Namun demikian, hasil tersebut masih lebih rendah dibandingkan dengan perlakuan MT (99,2%). Perlakuan akriflavin tidak mempengaruhi kelangsungan hidup ikan nila merah dan tidak berbeda nyata dengan kontrol, berkisar antara 91,7-95,0%.</p> <p>Kata kunci: Nila merah, <em>Orechromis</em> sp., monoseks, akriflavin</p>
Recovery Gonad Betina dan Kontrol Produksi Sperma Secara Hormonal pada Ikan Heterobranchus longifilis
Authors: Subagja Jojo , Gustiano Rudhy 
Published: February 2017

<p>Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first experiment was to evaluate the recovery of <em>Heterobranchus longifilis</em> females' gonad after induced ovulation, under tropical condition. Eggs diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 - 35 days after ovulation. The second experiment was carried out to improve milt production by hormone treatments, volume of intra testicular sperm and the numbers of spermatozoa were observed. The results indicated that the treatmens did not prove any significant differences.</p> <p>Keywords<em>: </em>hormone, gonad, reproduction<em>, Heterobranchus longifilis</em></p> <h1> </h1> <h2>ABSTRAK</h2> <p>Dua kegiatan penelitian dilakukan di Laboratorium IRD-Gamet, Montpellier, Perancis. Penelitian pertama dilakukan untuk mengetahui recovery gonad ikan <em>Heterobranchus longifilis</em> betina setelah ovulasi, di daerah tropis. Diameter telur digunakan sebagai indikator recovery gonad, diukur pada saat sekitar 28-35 hari setelah ovulasi. Penelitian kedua dilakukan untuk meningkatkan produksi sperma dengan memberikan perlakuan hormon. Volume semen dan jumlah spermatozoa dievaluasi. Hasil penelitian menunjukkan perlakuan tidak memberikan hasil yang signifikan.</p> <p>Kata kunci<em>: </em>hormon, gonad, reproduksi, <em>Heterobranchus longifilis</em></p>
Effect of Hardness in 3 ppt of Water Salinity on growth and survival rates of Barb Fish (Barbus conhonius Hamilton-Buchanan)
Authors: Nirmala Kukuh , Wulandari R. , Djokosetiyanto D. 
Published: February 2017

<p>This study was conducted to determine the effect of hardness on growth and survival rate of barb fish (<em>Barbus conchonius</em> Hamilton-Buchanan) reared in the 3 ppt of water salinity. Fish were reared in three different hardnesses, i.e., 54, 72,<sub> </sub>and 90 mg/L CaCO<sub>3 </sub>for 30 days. The results of study showed that fish reared in water hardness of 72 mg/L CaCO<sub>3</sub> had higher daily growth rate (1.15%) compared with other treatments. Daily growth rate of fish reared in water hardness of 54 and 90 mg/L CaCO<sub>3</sub> were 0.76 and 0.37%, respectively. There was no effect of hardness on survival rate of barb fish.</p> <p>Keywords: barb fish, <em>Barbus conchonius</em>, hardness, salinity</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini bertujuan untuk mengetahui pengaruh kesadahan yang berbeda terhadap laju pertumbuhan dan tingkat kelangsungan hidup ikan barbir (<em>Barbus conchonius</em> Hamilton-Buchanan) yang dipelihara <a name="OLE_LINK2"></a><a name="OLE_LINK1">pada air bersalinitas 3 ppt. </a>Ikan dipelihara dalam 3 tingkat kesadahan yang berbeda, yaitu 54 mg/L CaCO<sub>3</sub>, 72 mg/L CaCO<sub>3</sub>,<sub> </sub>dan 90 mg/L CaCO<sub>3 </sub>selama 30 hari. Hasil penelitian menunjukkan bahwa laju perumbuhan harian tertinggi diperoleh pada ikan yang dipelihara pada kesadahan 72 mg/L CaCO<sub>3 </sub>(1,15%) dibandingkan dengan perlakuan lainnya. Laju pertumbuhan harian ikan yang dipelihara pada kesadahan 54 dan 90 mg/L CaCO<sub>3</sub> masing-masing mencapai 0,76% dan 0,37%. Kelangsungan hidup ikan barb tidak dipengaruhi oleh kesadahan.</p> <p>Kata kunci: ikan barbir, <em>Barbus conchonius</em>, kesadahan, salinitas</p>
Effect of Enriched Feed by n-3 fatty acids and 2% of n-6 fatty acid on Danio rerio Reproduction
Authors: Utomo N.B.P , Nurmalia L. , Mokoginta I. 
Published: February 2017

<p>This experiment was conducted to determine the optimum n-3 fatty acid level in the diet containing 2 % of n-6 fatty acid on the reproductive performance of zebra fish (<em>Danio rerio</em>). There experimental diets containing 0.0; 1.0; 1.5 % n-3 fatty acid with 2.0 % n-6 fatty acid was fed to the fish, three times daily, at satiation, for two months. In order to evaluate the gonadal development of the broodstock, two gonads og fish was used for histologis preparation in every 7 days. At the end of the second month, reproductive performance was evaluated through parameters of gonad somato indeks, fecundity, fertilization rate, hatching rate, yolk egg absorbtion rate, survival rate of 3 days old larvae. Sample of fish also was taken for proximate composition as the end of this experiment. Results shows that at the fifth weeks of this experiment, gonad of fish fed on 1.0 % of n-3 fatty acid and 2.0 % n-6 fatty acid already produce eggs with the some size, while others. Still produce small size of eggs. It was found also that the whole body of fish fed an diet with 1.0% n-3 fatty acid contain the highest protein level compare to two other diets. Based on the evaluation of reproduction performance parameters, it was concluded that the optimum dietary level of n-3 fatty acid with 2.0 % n-6 fatty acid for <em>Danio rerio</em> was 0.81 - 0.90 %.</p> <p><em>Keywords</em>: essential fatty, acids, reproduction, zebra fish, <em>Danio rerio</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini bertujuan untuk menentukan kadar asam lemak n-3 optimum dalam pakan yang mempunyai kadar asam lemak n-6 tetap. Tiga macam pakan dengan kadar asam lemak n-3 berbeda yaitu 0.0; 1.0; dan 2.0 % diberikan pada ikan dengan bobot rata-rata 0.12 g. Pakan diberikan secara at satiation, 4 kali sehari selama 60 hari. Setiap 7 hari sekali diambil sampel ikan untuk pembentukan preparat histologi gonad dengan tujuan untuk mengevaluasi perkembangan gonad. Pada akhir penelitian, induk dipijahkan dan dievaluasi performan reproduksi berdasarkan parameter. Gonad Somato Indeks, fekunditas, derajat pembuahan, derajat penetasan dan kelangsungan hidup larva berumur 3 hari. Sampel tubuh ikan diakhir penelitian juga dievaluasi komposisi proksimatnya. Hasil penelitian ini memperlihatkan bahwa pada minggu ke-5 setelah pemberian pakan, gonad dari ikan yang diberi pakan asam lemak n-3 1.0 % mengandung telur-telur dengan ukuran yang seragam sedangkan ukuran telur ke dua perlakuan lainnya masih bervariasi. Kadar protein tertinggi juga terdapat pada tubuh ikan yang diberi pakan 1.0 % asam lemak n-3. Berdasarkan parameter performan reproduksi secara keseluruhan dapat disimpulkan bahwa kadar asam lemak n-3 optimum dalam pakan ikan zebra, <em>Danio rerio</em> adalah 0.81 - 0.90 %.</p> <p>Kata kunci: asam lemak, reproduksi, ikan zebra, <em>Danio rerio</em></p>
Effect of Thyroxine Hormone by Oral on Growth and Survival Rate of Coral Platy Xiphophorus maculates
Authors: Junior M. Zairin , Pahlawan R.G. , Raswin M. 
Published: February 2017

<p>Thyroxine is known as a hormone that can affect growth of fish by increasing the metabolic rate, feed efficiency and protein retention. In this study, effect of thyroxine administration through feed on growth and survival of coral platy fish (<em>Xiphophorus maculatus</em>) was observed. Dosage of thyroxine used was 0.2, 2 and 20 mg/kg of feed, and were combined with duration time of treatment i.e. 1, 2 and 3 weeks. All treatments were performed triplicate. The results of study show that higher growth in length (21.3 mm) and weight (244.5 mg) was obtained by administration of 20 mg thyroxine per kilogram of feed for two weeks and this treatment had no effect on the survival rate of coral platy fish</p> <p>Keywords: coral platy, <em>Xiphophorus maculatus</em>, tyroxine, growth</p> <p> </p> <p>ABSTRAK</p> <p>Tiroksin diketahui sebagai hormon yang dapat mempengaruhi pertumbuhan dengan jalan meningkatkan laju metabolisme tubuh, efisiensi makanan dan retensi protein. Pada penelitian ini dipelajari pengaruh pemberian hormon tiroksin melalui pakan terhadap pertumbuhan dan kelangsungan hidup ikan plati koral (<em>Xiphophorus maculatus</em>). Dosis tiroksin yang digunakan yaitu 0,2; 2 dan 20 mg/kg pakan, dan dikombinasikan dengan lama waktu perlakuan satu, dua dan tiga pekan. Semua perlakuan diulang tiga kali. Hasil penelitian menunjukkan bahwa pemberian hormon tiroksin sebesar 20 mg/kg pakan selama dua pekan memberikan pertumbuhan panjang dan pertambahan berat terbaik masing-masing 21,3 mm dan 244,5 mg dan tidak berpengaruh terhadap kelangsungan hidup ikan platy koral.</p> <p>Kata kunci: ikan plati koral, <em>Xiphophorus maculatus, </em>tiroksin, pertumbuhan</p>
Effect of 17α-Methyltestosterone Hormone Manipulation in Various Water Temperatures on Sex Ratio of Guppy (Poecilia reticulata Peters)
Authors: Arfah H. , Kadriah A.K. , Carman O. 
Published: February 2017

<p>This study was conducted to determine percentage of male progeny of guppy (<em>Poecila reticulata</em> Peters) broodstock immersed in water containing various dose of 17α-Methyltestosterone (MT) and different temperature. Immersion of broodstock was carried out at 10<sup>th</sup> day after spawning. Dosage of MT used was 0, 0.5 and 1 mg per liter of water, while the water temperature was 27, 30 and 33<sup>o</sup>C, for 24 hours immersion. The result of study showed that the combination of hormone treatment 1mg/L and temperature 27<sup>o</sup>C produced higher percentage of male progeny (92.7%). Increasing water temperature in combination with dose of hormone treatment can reduce percentage of male progeny.</p> <p>Keywords: guppy, <em>Poecilia reticulata</em>, 17α-Methyltestosterone, temperature, sex reversal, monosex</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui nisbah kelamin jantan keturunan induk ikan gapi (<em>Poecila reticulata</em> Peters) yang telah direndam hormon 17α-Metiltestosteron (MT) dengan berbagai tingkatan dosis yang dikombinasikan dengan temperatur air yang berbeda-beda. Perendaman induk dilakukan pada hari kesepuluh setelah pemijahan. Dosis hormon yang digunakan adalah 0, 0,5 dan 1 mg/L air, sementara suhu air perendaman adalah 27<sup>o</sup>C, 30<sup>o</sup>C dan 33<sup>o</sup>C, dengan lama perendaman 24 jam. Hasil penelitian menunjukkan bahwa kombinasi perlakuan hormon 1 mg/L dengan temperatur 27ºC menghasilkan rataan persentase ikan gapi kelamin jantan tertinggi yaitu 92,7%. Peningkatan temperatur yang dikombinasikan dengan dosis hormon ternyata mengakibatkan penurunan persentase anak berkelamin jantan.</p> <p>Kata kunci: ikan gapi, <em>Poecilia retiulata</em>, 17α-Metiltestosteron, temperatur, seks reversal, monoseks</p>