bluuebunny/crossref_metadata_2025_split huggingface dataset:
ID: 1084 Proliferative and differentiative effects of the Panax vietnamensis ethanol fraction on murine neural stem cells
Authors: Do Huy Quang , Truong Nhung Hai , Lam Thanh Thai , Nguyen Linh Thuy , Le Dung Minh , Dinh Nhung Thi-Hong , Tran Luan Cong , Phan Ngoc Kim , Pham Phuc Van 
Published: September 2017

Background:Ginseng has been known since ancient time for its unique pharmaceutical effects on human health. Modern studies indicated that extracts of ginseng could improve a broad range of pathological conditions including neurodegenerative diseases such as Alzheimer’s or Parkinson’s disease. Panax vietnamensis, a new ginseng species recently discovered in Vietnam, has received much interest due to its high composition of both known and new saponins whose therapeutic effects have not yet fully evaluated. In this study, the proliferative and differentiative effects of different fractions of the extract of Panax vietnamensis on cultured neural stem cells would be assessed.
Materials and methods:
Fractions from the Panax vietnamensis were prepared following a successive extraction of the raw material using ethanol, n-butanol, ether ethylic, and water, respectively. Potential toxicity and proliferative effects of each fraction were assessed based on the increase in the sphere diameter in before further analyses. The percentage of cells in active phases, cell cycle- or differentiation-associated genes were analyzed using flow cytometry and real-time RT-PCR.
Results:Our results revealed that supplementing media with 200 µg/ml of ethanol Panax vietnamensis fraction significantly increased diameters of neurospheres, also maintained the growth rate of the neurospheres compared with the control within the five-day period. Moreover, the results also demonstrated that the ginseng ethanol fraction and a known neurotrophic protein, NGF, affected the differentiation of the neural stem cells.

Conclusion:200 µg/ml ethanol fraction from the extract of Panax vietnamensis most significantly induced the proliferation of cultured neural stem cells. However, the higher concentration of the fraction seemed not to maintain the growth of neurospheres. Regarding differentiation, the ethanol fraction of Vietnamese ginseng possibly shared similar mechanisms with the neurotrophic growth factor.
DO PRIVATE SECURITY OFFICERS NEED ADDITIONAL LEGAL POWERS TO HELP POLICE REDUCE CRIME IN GAUTENG, SOUTH AFRICA?
Authors: Kole Olaotse John 
Published: September 2017

There is a need for different stakeholders to work together to help the police combat crime in Gauteng, South Africa. Private security officers are usually well positioned to help combat crime because they can witness crime in areas where they are posted or are patrolling as response officers. Private security officers protect organisations (public and private) or individuals who are their paying clients. But they can only perform their duty as ordinary citizens, not as the police would. In looking at how these officers can be more effective, this article establishes to what extent private security officers need additional legal powers, and what powers would be suitable for them in helping the police combat crime. Mixed methods were used in this study of 20 police stations and 20 private security companies in Gauteng. From top management levels in the South African Police Service (SAPS), 37 high-ranking police officers participated in the study and 30 high-ranking security officers from top management level of the private security industry (PSI). At the operational levels, 173 SAPS officers completed the questionnaires and 163 PSI officers. The findings show that the majority of respondents see no need for private security officers to be given additional powers to help the police combat crime. However, the few respondents who supported the idea think that the power to arrest, and stop and search the public, should be given to private security personnel. At the operational level, all respondents agreed that private security officers should be given additional legal powers to help the police combat crime. :
Проект структуры культурологического знания
Authors: Флиер Андрей Яковлевич 
Published: September 2017

<p>В статье анализируется многообразие взглядов ученых на место культурологии в отечественной науке и образовании и выстраивается вариант ее структуры, складывающийся, по мнению автора, фактически в практике научных исследований в России.</p><p>Рассматривается разнообразие существующих точек зрения на познавательные задачи культурологии, на разные сегменты социально важного знания, которое она добывает или должна добывать. Анализируются разные взгляды на то, с какими науками культурология имеет наибольшую общность.</p><p>Выстраивается модельная схема содержательной структуры культурологического знания, не вполне соответствующая традиционной «академической» структуре этой области знания, но, по мнению автора, больше соответствующая фактической научной практике исследований. По авторской схеме культурологическое знание членится на три уровня: первый — отрасли знания, различающиеся главным образом методологически; второй — проблемные направления знания, различающиеся социальными проблемами, решаемыми в данном сегменте; и третий — предметные области знания, различающиеся сущностью изучаемых артефактов. По этому принципу рассматриваются семь основных сегментов современного культурологического знания и их связи со смежными отраслями современной науки.</p><p>Актуальность предлагаемой модели заключается в том, что таким образом мы можем углубить свои представления об основаниях социальной интеграции человеческого общества, о причинах культурного многообразия человечества и о закономерностях исторической динамики культуры. </p>
Fine localization of the sym31 locus in pea linkage group III
Authors: Tsyganov Viktor E , Rozov Sergey M , Knox Maggie , Borisov Aleksey U , Ellis Tomas N , Tikhonovich Igor A 
Published: September 2017

Analysis of joint inheritance of symbiotic locus sym31 and 12 molecular and morphological markers of pea linkage group III was performed. The linkage between symbiotic locus sym31 and 11 analyzed markers was observed. Using theAntMap software,adetailed genetic map of the sym31 locus was constructed and its fine position in linkage group III was determined.
Linked symbiotic populations: analysis of genetic diversity of rhizobial component
Authors: Muntyan Aleksey N , Andronov Evgeniy E , Belova Viktoriya S , Roumiantseva Marina L , Simarov Boris V 
Published: September 2017

Understanding the selective constraints of partner specificity in mutually beneficial symbiosis is a significant, yet largely unexplored, prospect of evolutionary biology. These selective constraints can be explored through the study of nucleotide polymorphism at loci controlling specificity. We used a model legume-rhizobium mutualism to test for evidence that contextdependent selection may maintain variation in partner quality. We analyze the taxonomic structure, the heterogeneity and linkage disequilibrium of the rhizobial population between symbiotic (nif-, fix- and nod-, nodL-noeA genes) and chromosomal (leu- and IGS loci) genetic markers.
THE PEA GENE Crt CONTROLING THE MORPHOGENETIC RESPONSE OF THE ROOT IS INVOLVED IN REGULATION OF ACC-OXIDASE ACTIVITY
Authors: Zhernakov Alexander I , Tsyganov Viktor E , Borisov Aleksey U , Tikhonovich Igor A 
Published: September 2017

The ethylene status of the pea mutant SGEcrt with altered morphology of the root system, whose development is dramatically dependent on the mechanical conditions of the environment, was studied. The role of ethylene in phenotypic manifestation of mutant root system was confirmed. It was shown that the mutant is characterized by increased production of and increased sensitivity to ethylene and by changes in regulation of ethylene biosynthesis leading to increased activity of the ethylene-producing enzyme ACC-oxidase.
ENVIRONMENTAL STRESS AND MUTAGENESIS IN ENTERIC AND NON-ENTERIC BACTERIA
Authors: Babudri Nora , Lancioni Hovirag , Achilli Alessandro 
Published: September 2017

Mutations are fundamental for evolution. For many years it has been thought that mutagenesis occurs only in dividing cells. Now it is clear that mutations arise in non-dividing or slowly dividing microorganisms. Natural populations spend most of the time in stressful environments where their growth rate is highly reduced. Thus, the existence of a mutagenesis process, independent of multiplication (stress-induced mutagenesis, SIM), might have a profound evolutionary role. In the presented paper we review the stateof-the-art in enteric and non-enteric bacteria. We describe different experimental systems as well as the mechanisms and models presented to explain the huge amount of data obtained in more than twenty years of research.
STUDYING THE STRUCTURE OF SOIL MICROBIAL COMMUNITY IN SALINE SOILS BY HIGH-THROUGHPUT PYROSEQUENCING
Authors: Pershina Elisaveta V , Tamazyan Gaik S , Dolnik Alexandr S , Pinaev Alexander G , Sergaliev Nurlan H , Andronov Evgeniy E 
Published: September 2017

The taxonomic structure of soil microbial community was studied in six samples taken from a salt marsh along the salinity gradient and in two samples of non-saline soils using pyrosequencing method (454 Roche). The analysis allowed to identify three main ecological groups of microorganisms depending on the degree of the soil salinity. Halophylic microorganisms were mainly represented by bacteria of three phyla Firmicutes, Proteobacteria and Bacteroidetes and included much less of archaea (the Halobacteriaceae family). Within the distance of 150–200 m from the point with the highest levels of salinity, the microbial community tends to have a considerable similarity with control samples of non-saline soils.
Phylogenetic analysis of perfect repeats in the genomes of unicellular cyanobacteria
Authors: Mikheeva Lidia E , Orlov Yuri L , Kolchanov Nikolay A , Shestakov Sergey V 
Published: September 2017

We have fulfilled in silico research of number, structure, distribution and location of direct and inverted repeated sequences in eight complete genomes of unicellular cyanobacteria. Analysis of whole genome repeats has shown utility of this approach for purposes of molecular phylogeny and ecological genomics. Comparative analysis of nonrandom repeats patterns has allowed: 1) to confirm the close genetic relationship of two Prochlorococcus marinus strains (MED4 and SS120) that have reduced genomes and inhabit the econiches with different light intensities; 2) to suggest the close phylogenetic relationship of genomes Prochlorococcus marinus MIT9313 and Synechococcus WH8102 that significantly differ by sets of lightharvesting photosystem; 3) to reveal specific differences in genome organization between marine and freshwater cyanobacteria.
Increase of efficiency of QSAR — the analysis of carcinogenic activity of halogeneted hydrocarbons
Authors: Tarasov Aleksey V , Abilev Serikbay K , Velibekov Ramis M , Tarasov Valentin A 
Published: September 2017

The new principle of analysis of structureactivity of carcinogenic compounds is offered. It is basis on use a compound structural descriptors representing a combination untied among themselves of structural fragments of a molecule. The computer program is developed, allowing to generate every possible structural fragments of molecules of chemical compounds and their combinations and to carry out selection of the compound descriptors statistically significantly influencing their biological activity. In work it is used the samples consisting of 89 halogeneted hydrocarbons for which their carcinogenic activity on rodents is investigated.
VARIABILITY OF PRONOTUM PATTERNS IN LADYBIRD BEETLE HARMONIA AXYRIDIS PALLAS (COLEOPTERA, COCCINELLIDAE)
Authors: Blehman Alla V 
Published: September 2017

The polymorphism of pronotum patterns in ladybird beetle Harmonia axyridis was studied, 14 phenotypes of this character were identified for the first time. The contingency of pronotum variability with elytral patterns were investigated. For the first time the sexual dimorphism for pronotum patterns was revealed, and its peculiarity was analyzed. The analysis of geographic variability of pronotum patterns confirms the hypothesis for existence of two subspecies in H. axyridis.
Melatonin influence on Drosophila melanogaster life span at different light regimes
Authors: Moskalev Aleksey A , Krementsova Anna V , Malysheva Olga A 
Published: September 2017

It was investigated the combined effects of different light regimes (round-the-clock lighting or darkening) and antioxidant melatonin on Drosophila melanogaster life span of wild type strain Canton-S,mutant strain with defect of red-ox system (Sod) and mu tant strain with disturbance of excision DNA repair (mus210). It was revealed, that maintenance of Drosophila imago at round-the-clock lighting leads to decrease of mean and/or maximum life span with compare to being at darkening. Adding of melatonin to Drosophila meal induces the geroprotector effects, mainly, in dark conditions. The effects mentioned above were most expressed in strain with mutation of Sod. All effects have revealed in both sexes. The data analysis has shown that mechanisms of different light regimes and melatonin influ ence on life span are rather different.
Hyper-recombinogenity of the chimeric protein RecAX53 (Esherichia coli/Pseudomonas aeruginosa) is caused by its increased dynamics
Authors: Chervyakova Daria B , Lanzov Vladislav A 
Published: September 2017

RecAX53 is the most recombinogenic protein among the chimeric RecA proteins composed ofEsherichia coli RecA (RecAEc) and Pseudomonas aeruginosa RecA (RecAPa) protein fragments. We found out that RecAX53 protein is more rapid in ATP hydrolysis, dissociation from single-stranded DNA (ssDNA), SSB protein displacement from ssDNA and in association with doublestranded DNA (dsDNA), as compared with RecAEc and RecAPa proteins. These results indicate that the RecAX53 hyper-recombinogenity is caused by high dynamics of this protein - by its rapid association with and dissociation from ssDNA. The ability of RecAX53 to bind actively with dsDNA accounts for the SOS-independent mechanism of hyper-recombination used by this protein.
The analysis of association of the minisatellite UPS29 with Parkinson's disease
Authors: Suchkova Irina O , Shubina Daria M , Yakimovsky Andrey F , Borisova Elena V , Eliseeva Nadezhda G , Sasina Ludmila K , Baranova Tatyana V , Baranov Vladislav S , Patkin Eugene L 
Published: September 2017

The aim of this work was to identify new genetic markers associated with different forms of Parkinson's disease. A frequency of occurrence of different allele variants of minisatellite UPS29 localized in intron of centaurin p5 gene (CENTB5) was evaluated for patients with this pathology. The increase of frequency of UPS29 short alleles was observed for Parkinson's disease patients. This value depended on patient sex and age of pathology debut. Statistically significant difference with control was found only for females with early (30-50 years old) and late ( 60 years old) onset of Parkinson's disease. We suppose that UPS29 might be used as new genetic markers forearly (presymptomatic) diagnostics of some forms of Parkinson's disease.
Diludine and cerebrocrast as bioprotectors in the model test-systems in vivo
Authors: Savina Natalia V , Nikitchenko Natalia V , Dalivelya Olga V , Kuzhir Tatyana D , Bisenieks Egils , Duburs Gunars , Goncharova Roza I 
Published: September 2017

An influence of two 1,4-dihydropyridine derivatives (diludine and cerebrocrast) on Drosophiladevelopment and germ cell mutability was studied. It was revealed the concentration range, within which the compounds manifest their bio-stimulating effects increasing individual survival by 50-80 % as well as the protective action against the alkylating agent ethyl methanesulfonate reducing the level of induced mutations by 30-50 %. The pattern and presumable mechanisms of the bioprotective action of these compounds are considered.
Cytogenetic anomalies in gastric epithelial cells of fundic stomach region of the patients with chronic gastritis
Authors: Kitaeva Ludmila V 
Published: September 2017

The stomach is one of the microecological systems of human organism. Helicobacter pylori (HP) plays especial role in gastral ecosystem. The micronucleus (MN) test for investigation of cytogenetical anomalies in fundic stomach cells in chronic gastritis patients of different age was applied. Some morphological features — protrusions for example and presence of two nuclei in mucosa cells were registrated. Marked increase of cells with MN in HP-positive patients in comparison with non-positive ones in all age groups was found. The quantity differences of dinuclear parietal cells in HP-positive patients were revealed.
Double Mutant Analysis of Sequential Functioning of Pea (Pisum sativum L.) Genes: Sym13, Sym33 and Sym40 During Symbiotic Nodule Development
Authors: Tsyganov Viktor E , Seliverstova Elena V , Voroshilova Vera A , Tsyganova Anna V , Pavlova Zlata B , Lebsky Vladimir K , Borisov Aleksey U , Tikhonovich Igor A , Brewin Nicholas 
Published: September 2017

Two double mutants carrying pea symbiotic gene pairs sym13, sym40 and sym33, sym40, respectively, were constructed using single mutants blocked at different nodule developmental stages: E135f (sym13), SGEFix–‑1 (sym40) and SGEFix–‑2 (sym33). The epistasis of the mutant allele sym40 over the mutant allele sym13 and sym33 over sym40 was shown with respect to nodule histological and ultrastructural organisation. Thus, the proposed earlier sequential functioning of genes during infection process: Sym33→Sym40→Sym13 has been confirmed.
LTS3 gene controls light-independent chlorophyll biosynthesis in green algae Chlamydomonas reinhardtii
Authors: Chekunova Elena M , Savelieva Natalya V 
Published: September 2017

The genetic control of light-independent chlorophyll biosynthesis in plant cells has been investigated using Chlamydomonas reinhardtii Lts3-mutants defective in dark chlorophyll biosynthesis on the stage before protochlorophyllide to chlorophyllide conversion. In heterotrophic conditions the mutants are unable to synthesize chlorophyll and accumulate protoporphyrins, after illumination they are greening. The mutants were tested for pigment contents, activity of enzymes and expression of the genes, encoding these enzymes. The LTS3 gene has been identified by positional cloning, and the predicted LTS3 protein appeared to be a GATA transcription factor, which activate the expression of genes encoded chlorophyll biosynthesis enzymes: Mg-chelatase and glutamate 1-semialdehyde aminotransferase in the dark, and possibly, important for adaptation of plant cells for autotrophic conditions.
METABOLISM OF OSTEOTROPIC TOXICAL SUBSTANCES: HEREDITARY DETERMINATION
Authors: Starichenko Vera I 
Published: September 2017

The hereditary component of the variability (intrafamily correlation) of the metabolism of osteotropic toxical substances — sup90/supSr (a single injection) and stable fluorine (a chronic entering) were evaluated in laboratory linear mice in comparison with correlation of the morphological signs, hereditary of development of which is known. The evaluation was made with the control of the effects of sex, age and linear membership of animals and also taking into account the conditions of their development and the size of offspring. Significant intrafamily correlation of the concentration of sup90/supSr and of fluorine in bone tissuewas revealed (r = 0,4–0,5; p 0,0001). It can be compared with the correlation of morphological signs, which is the evidence of hereditary determinacy of the metabolism of osteotropic toxical substances.
Characterization of variability of the gene Nad1 intron in representatives of genus Vicia L. (Fabaceae Lindl.)
Authors: Ryzhova Natalia N , Dyachenko Elena A , Vishnyakova Margarita A , Kochieva Elena Z 
Published: September 2017

For the first time the sequences of b/c intron of mitochondrial gene Nad1 have been determined in representatives of 13 Vicia species. It is shown that the sequences of the intron of the gene in these species is highly conservative. In total 23 variable sites and 11 indels have been determined with the intron length 1421–1447 b. p. On the basis of the results of pre-m-RNA intron folding the main elements of its secondary structure have been determined as well as the sites of interdomain interactions. The peculiarities of primary and secondary structure of intron Nad1 in Vicia have been characterized.
Genetical structure of populations of modelling species of terrestrial mollusks in conditions of the urbanized landscape on example Сhondrula tridens Müll (Gastropoda, Pulmonata)
Authors: Snegin Eduard A 
Published: September 2017

On the basis of the analysis of the morphological and genetical variability revealed by a method of gel-electrophoresis of proteins in PAAG, the state of gene pools of populations of modeling species Сhondrula tridens Müll (threedens snail) in conditions of the urbanized forest-steppe landscape of the south of Mid-Russia Upland is investigated. In the majority of the investigated bunches authentic decrease of a level of heterozygosity and the decrease of an allelic diversification caused, both is fixed by natural historical factors, and factors of an anthropogenic parentage. The genetic-automatic processes in populations are considered and vectors of natural selection are defined. Calculation of effective number of researched bunches is spent.
Gene polymorphism, ecogenetic diseases and predictive personalized medicine
Authors: Baranov Vladislav S 
Published: September 2017

The problems concerned with identification of genes involved in the origin of complex diseases, analysis of their epistatic (gene to gene) interactions and adequate interpretation of genetic testing results in Predictive Personalized Medicine (PPM) are reviewed. The practical meaning of already available PPM data, the options and volume of their feasible clinical implications are discussed.
Insights into How HIAs are Characterized in the Press: Findings from a Media Analysis of Widely Circulated United States Newspapers
Authors: Gakh Maxim , Coughenour Courtney , Pharr Jennifer , Goodie Aaliyah , To Samantha 
Published: September 2017

<p class="Normal1"><strong>Background: </strong>Health impact assessments<strong> </strong>(HIAs) are burgeoning tools in the policy process, where the media plays a critical role by focusing attention on issues, informing consumers, and influencing positions. Examining how media portrays HIAs is critical to understanding HIAs in the policy context.</p><p class="Normal1"><strong>Methods: </strong>This study considered how widely circulated, U.S. newspapers represent HIAs. After searching newspaper databases, we used a qualitative document analysis method consisting of open and axial coding to examine specific phrases of HIA depictions.<strong> </strong></p><p class="Normal1"><strong>Results: </strong>In coding over 1,000 unique phrases from the 62 documents generated in our search, we found an uptick in HIA-related publications since 2010. Coding these documents identified 46 distinct codes across 10 different themes. The two most prominent HIA-centered themes focused on HIA engagement and the HIA setting. While themes of policy and science, health determinants, and explanations of HIAs were also frequently featured, specific mentions of projected impacts, HIA processes, HIA values, and health outcomes were less prevalent.</p><p class="Normal1"><strong>Conclusions: </strong>HIA media portrayals warrant further inquiry from researchers and practitioners. Focusing on how media portrays HIAs is consistent with several HIA steps. It is also important for a broader strategy to educate stakeholders about HIAs and to understand HIAs’ utility. HIA practitioners should develop and implement guidelines for media interaction and tracking that encourage practitioners to seek additional media attention and to focus such attention on health impacts and outcomes, HIA recommendations, and HIA values. Building on our work, researchers should examine HIA media portrayals beyond the context of this study.</p>