bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple poliovirus-induced organelles suggested by comparison of spatiotemporal dynamics of membranous structures and phosphoinositides
Authors: Oh Hyung S. , Banerjee Sravani , Aponte-Diaz David , Aligo Jason , Lodeiro Maria F. , Cameron Craig E. 
Published: May 2017

ABSTRACTPoliovirus (PV) infection induces membranes with elevated levels of phosphatidylinositol-4-phosphate (PI4P) and invaginated, tubular structures that appear as vesicular clusters in cross section. Here, we characterize PV mutants, termed EG and GG, which exhibit aberrant proteolytic processing of the P3 precursor that can delay the onset of genome replication and/or impair virus assembly. For WT PV, changes to the PI4P pool were observed as early as 30 min post-infection. PI4P remodeling occurred even in the presence of guanidine hydrochloride, a replication inhibitor. Vesicular clusters were not apparent until 3 h post-infection, a time too slow for these structures to be responsible for genome replication. Delays in the onset of genome replication observed for EG and GG PVs were explained completely by the reduced kinetics of virus-induced remodeling of PI4P pools, consistent with PI4P serving as a marker of the genome-replication organelle. Infectious virus produced by GG PV is known to be reduced nearly 5 logs. We show that GG PV was unable to make virus-induced vesicular clusters. Instead, GG PV-infected cells accumulated elongated tubules. Our results are consistent with the existence of distinct organelles for genome-replication and virus assembly. We suggest that the pace of formation and spatiotemporal dynamics of PV induced organelles may be set by the rate of P3 precursor processing to form 3AB and/or 3CD proteins.AUTHOR SUMMARYAll positive-strand RNA viruses replicate their genomes in association with host cell membranes. PV does not just remodel existing membranes, but induces membranes with unique structure and lipid composition. There has been some suggestion that the functions of the PV induced structures observed during infection may not be those that perform genome-replication. This study uses kinetic analysis of virus-induced membrane formation and PI4P induction by two PV mutants to provide evidence for the existence of a virus-induced genome-replication organelle distinct from a second organelle, the absence of which impairs virus assembly. In addition, our studies suggest that formation of both organelles may require participation of viral proteins, 3AB and/or 3CD. Therefore, this study provides a new perspective on the cell biology of PV infection and should inspire a fresh look at picornavirus-induced organelles, their functions and the role of P3 proteins in their formation.
Wolbachiaeffects on Rift Valley fever virus infection inCulex tarsalismosquitoes
Authors: Dodson Brittany L. , Andrews Elizabeth S. , Turell Michael J. , Rasgon Jason L. 
Published: May 2017

AbstractInnovative tools are needed to alleviate the burden of mosquito-borne diseases, and strategies that target the pathogen instead of the mosquito are being considered. A possible tactic is the use ofWolbachia, a maternally inherited, endosymbiotic bacterium that can suppress diverse pathogens when introduced to naive mosquito species. We investigated effects of somaticWolbachia(strainwAlbB) infection on Rift Valley fever virus (RVFV) inCulex tarsalismosquitoes. When compared toWolbachia-uninfected mosquitoes, there was no significant effect ofWolbachiainfection on RVFV infection, dissemination, or transmission frequencies, nor on viral body or saliva titers. WithinWolbachia-infected mosquitoes, there was a modest negative correlation between RVFV body titers andWolbachiadensity, suggesting thatWolbachiamay suppress RVFV in a density-dependent manner in this mosquito species. These results are contrary to previous work in the same mosquito species, showingWolbachia-induced enhancement of West Nile virus infection rates. Taken together, these results highlight the importance of exploring the breadth of phenotypes induced byWolbachia.Author SummaryAn integrated vector management program utilizes several practices, including pesticide application and source reduction, to reduce mosquito populations. However, mosquitoes are developing resistance to some of these methods and new control approaches are needed. A novel technique involves the bacteriumWolbachiathat lives naturally in many insects.Wolbachiacan be transferred to uninfected mosquitoes and can block pathogen transmission to humans. Additionally,Wolbachiais maternally inherited, allowing it to spread quickly through uninfected field populations of mosquitoes. We studied the impacts ofWolbachiaon Rift Valley fever virus (RVFV) in the naturally uninfected mosquito,Culex tarsalis.Wolbachiahad no effects on the ability ofCulex tarsalisto become infected with or transmit RVFV. High densities ofWolbachiawere associated with no virus infection or low levels of virus, suggesting thatWolbachiamight suppress RVFV at high densities. These results contrast with our previous study that showedWolbachiaenhances West Nile virus infection inCulex tarsalis.Together, these studies highlight the importance of studyingWolbachiaeffects on a variety of pathogens so that control methods are not impeded.
A neuropeptide signaling system that rapidly enforces paternity in theAedes aegyptimosquito
Authors: Duvall Laura B. , Basrur Nipun S. , Molina Henrik , McMeniman Conor J. , Vosshall Leslie B. 
Published: May 2017

AbstractFemale Dengue and Zika vector mosquitoes (Aedes aegypti)generally mate once, with sperm from this male fertilizing all eggs produced in her lifetime. Here we implicate HP-I, anAedes- and male-specific neuropeptide transferred to females, and its cognate receptor in the female, NPYLR1, in rapid enforcement of paternity.HP-Imutant males were ineffective in enforcing paternity when a second male was given access to the female within 1 hour.NPYLR1mutant females produced mixed paternity offspring at high frequency. Synthetic HP-I injected into wild-type virgins reduced successful matings, but had no effect onNPYLR1mutant females. Asian tiger mosquito (Ae. albopictus) HP-I potently activatedAe. aegyptiNPYLR1. InvasiveAe. albopictusmales are known to copulate with and sterilizeAe. aegyptifemales, and cross-species transfer of HP-I may contribute to this phenomenon. This neuropeptide system promotes rapid paternity enforcement withinAe. aegypti, but may promote local extinction in areas where they compete withAe. albopictus.One Sentence SummaryAedes-specific peptide rapidly enforces paternityTextAe. aegyptifemales typically mate only once with one male in their lifetime, a behavior known as “monandry” (1). This single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4-6 week lifespan in the laboratory (2). Successful mating is capable of inducing lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male (3-5). In other species, males use diverse strategies to assure the paternity of their offspring, for instance physical barriers such as mating plugs found in mice (6) andAnophelinemosquitoes (7), and anti-aphrodisiac pheromones used byDrosophila melanogastermales to tag female flies as non-virgin (8). Another widely used strategy in insects is the transfer of biologically active male seminal proteins, produced by the male accessory gland and secreted into the ejaculatory duct along with sperm during insemination, to affect the sexual receptivity of the female (3, 9-13). Perhaps the best-characterized male seminal fluid protein in insects is theDrosophilafly sex peptide (11), which acts on the sex peptide receptor in the female to suppress receptivity and trigger egg production (12).Drosophilasex peptide receptor mutant females will readily remate with multiple males, and wild-type females that mate with sex peptide mutant males remain sexually receptive.
Evaluating app bundling strategy for selling mobile apps: an ambivalent perspective
Authors: Wan Jinlin , Zhao Ling , Lu Yaobin , Gupta Sumeet 
Published: May 2017

Purpose
Mobile appstores have fast sprung up during the last few years. The large number of apps in these appstores results in increased search effort for the customer as well as fierce competition leading to poorer revenues for both appstores as well as developers. Therefore, appstores allow developers to resort to bundling apps so as to increase the revenues and improve customer loyalty. Since an app-bundle is a mix of two or more apps, it may induce both positive and negative emotions simultaneously in the consumer. The purpose of this paper is to examine the effectiveness of app-bundling strategy from consumers’ perspective, and help developers design app-bundles.

Design/methodology/approach
Based on ambivalence theories, this research investigate different antecedents and influence of positive attitude and negative attitude on purchase behavior, and derives seven key app-bundles attributes through an exploratory study. The data were collected from 930 consumers of app-bundles in China and analyzed using SEM approach.

Findings
The findings indicate that positive attitude and negative attitude are two separate concepts and the identified seven app-bundling attributes have distinct effect on shaping consumers’ positive and negative attitude.

Originality/value
This study makes three key contributions to theory and practice. First, this study identifies the specific attributes of app-bundles using exploratory study. Second, this study addresses the challenges involved in examining bundles using ambivalence theory. In doing so, it characterizes attitude as positive and negative and treats them as separate constructs. Third, as called forth by previous studies, this study establishes the co-existence of positive attitude and negative attitude.
The moderating effects of organisational culture on the relationship between absorptive capacity and software process improvement success
Authors: Lee Jung-Chieh , Chen Chung-Yang , Shiue Yih-Chearng 
Published: May 2017

PurposeThe purpose of this paper is to identify the relationships among absorptive capacity (AC), organisational culture (OC), and software process improvement (SPI) implementation success based on capability maturity model integration (CMMI). Specifically, the aim of this study is to understand the roles of potential and realised AC in SPI success.Design/methodology/approachThis study proposes a research model and examines the relations among firm AC, SPI success, and OC. The authors surveyed 56 Taiwanese firms that adopted the CMMI-based SPI programme and received official certification.FindingsThe findings indicate that potential and realised AC have significantly positive effects on SPI success. In particular, realized (AC) partially mediates the effects of potential AC on SPI success. Furthermore, the effects of potential and realised AC on SPI success are significantly moderated by the adhocracy and hierarchy OCs, respectively.Research limitations/implicationsGiven that the scope of this empirical study was limited to Taiwan, the findings might lack generalisability; however, this provides an opportunity for future research.Originality/valueThis is the first empirical study investigating the influence of AC on CMMI-based SPI success. The proposed research model also examines the moderating relationships of two types of OCs, namely adhocracy and hierarchy, on AC (potential and realized) and SPI success.
Universities’ third mission and the entrepreneurial university and the challenges they bring to higher education institutions
Authors: Rubens Arthur , Spigarelli Francesca , Cavicchi Alessio , Rinaldi Chiara 
Published: May 2017

Purpose
Over the past few decades, higher education institutions (HEIs) have become key players in regional economic development and knowledge transfer, which has led to a third mission for HEIs and the entrepreneurial university. The purpose of this paper is to assess the challenges of HEIs in fulfilling the third mission for economic development and the changing role of being an entrepreneurial university, and the changes that need to be implemented to fulfill this new mission.

Design/methodology/approach
The authors have drawn on current literature to examine academic entrepreneurism and the entrepreneurial university, and how universities are fulfilling their third mission.

Findings
The findings from our review of the literature demonstrated the varied economic and social benefit of universities conducting external third mission/entrepreneurial activities in the community, as well as how the changing role and expectations of universities to become more entrepreneurial, has not only changed the expectations and role of university administrators, faculty and staff but also the business community which they serve. The review also showed the varied challenges for universities in fulfilling the third mission of economic development.

Research limitations/implications
Although ample literature and cases about universities’ third mission of economic development and the new entrepreneurial university (especially with research universities) were available, literature or research was limited on the specific challenges and obstacles faced by administrators, faculty and departments in fulfilling this mission, and few studies recommended changes that needed to be implemented in HEIs to support this new mission.

Practical/implications
The paper supports the potential role that HEIs play in implementing economic development in their communities or region. The paper also highlights some of the necessary resources and policy changes that policymakers and university administrators need to implement to reward and recognize faculty in conducting outreach activities as part of the university’s third mission.

Originality/value
The findings from this study highlight the challenges and barriers for faculty, staff and HEIs in fulfilling the third mission and becoming an entrepreneurial university.
Content analysis within intangible assets disclosure: a structured literature review
Authors: Castilla-Polo Francisca , Ruiz-Rodríguez Consuelo 
Published: May 2017

PurposeIn this paper, the authors analyze the use of content analysis in disclosing voluntarily information on intangible assets, the intangible assets disclosures (IAD). The purpose of this paper is to conduct a structured literature review (SLR) that assesses the possibilities and limitations of content analysis.Design/methodology/approachTo that end, the authors analyze the existing literature on the topic in the main international databases. In all, 74 empirical articles utilizing content analysis as a research methodology for IAD were reviewed. Regarding the selection of sources, the authors should indicate that the SLR performed includes academic studies published in journals or presented at conferences and that are always subject to a double process of anonymous review.FindingsThe obtained results indicate that despite the frequent use of content analysis in studies on IAD, its use does not meet all expectations.Research limitations/implicationsThe study synthesizes the research on content analysis for the case of information on intangible assets, offering an updated and global framework for future researchers through the SLR.Practical implicationsAmong other problems, the authors found its excessive emphasis on the amount disclosed in the annual report, ignoring other reports in which more information regarding intangible assets is available, such as in the case of the sustainability reports. Furthermore, the use of very different coding systems and its exclusive use without being combined with other methodologies are detected. These aspects affect the quality problems of the sources used, which directly results in the utility of the evidenced findings.Social implicationsThese conclusions allow the authors to conclude on the need to open different lines of study that review the use of content analysis in this topic.Originality/valueThe work focuses on the quality of disclosures more so than on the quantity, offering a critical view that summarizes the utility of the employment of content analysis for this type of disclosure and its implications for future research on this topic. Despite previous studies, the authors highlight the new insights revealed from IAD research, especially since the seminal paper of Dumay and Cai (2014).
Representation of intellectual capital’s components amongst Italian social enterprises
Authors: Benevene Paula , Kong Eric , Barbieri Barbara , Lucchesi Massimiliano , Cortini Michela 
Published: May 2017

Purpose
The purpose of this paper is to understand the representation that senior managers of Italian social enterprises have about their organization’s intellectual capital (IC), precisely about the human capital, relational capital and organizational capital.

Design/methodology/approach
This paper used a qualitative approach. A total of 81 senior managers were interviewed individually. Interview data were analyzed using different techniques of content analysis, particularly by using the T-Lab software (analysis of word occurrence and co-word mapping, analysis of Markovian sequences).

Findings
Findings confirm the divide between theory and practice of IC. The representation of the IC dimensions is rather different from the definition that is found in the academic literature. Limited awareness about IC components and their generative power of knowledge determines a limited exploitation of the social enterprises’ organizational knowledge.

Research limitations/implications
The group reached is limited to Italy and is not statistically representative of all Italian social enterprises.

Practical implications
Social enterprises are crucial in the development and well-being of societies. However, the findings suggest that many social enterprises managers are not fully aware of the importance of IC and how it may create value for their organizations. This paper stresses that senior managers of social enterprises need to, through various methods, have a better understanding of IC management and knowledge creation if they are to fully utilise the potential of IC in their organizations for survival and growth.

Originality/value
This is the first attempt to explore the perception of IC’s components among social enterprises, which represent an important development of non-profit organizations.
Healthcare sustainability and the role of intellectual capital
Authors: Cavicchi Caterina 
Published: May 2017

Purpose
The purpose of this paper is to investigate the role of intellectual capital (IC) in promoting the sustainable development (SD) program of the Emilia-Romagna Health Service. The contributions of the following assets were investigated: leadership and competences, culture, performance measurement and incentives systems, social capital and technologies.

Design/methodology/approach
The case study was conducted following a hierarchical approach: perceptions of the regional directorate of public and social health, the general directors and healthcare professionals of the regional health system (the setting) were analyzed through interviews, focus groups and documentation in order to investigate: the emerging definition of SD within the setting; the role of IC, if any, in the achievement of the regional SD goals.

Findings
SD culture did not expand at the operative level because of the lack of involvement of healthcare professionals in a permanent dialogue for sustainability. Sustainability projects were not systematic which restricted the development of staff awareness of sustainability issues. Social capital enabled environmental projects and medical projects that increased patients’ involvement in disease management. Technology could help the shift toward sustainability, but it requires consideration of tangible and intangible costs for its successful adoption. SD performance measurement and incentives were in their infancy and cost accounting continues to dominate the healthcare sustainability debate.

Research limitations/implications
Despite the low number of healthcare professionals involved in the focus groups, the paper represents one of the first attempts to frame their perceptions on SD implementation in healthcare.

Practical implications
Regional institutions should consider new ways of enacting SD which should be more inclusive of healthcare professionals. The establishment of a permanent interdisciplinary dialogue on sustainability would develop human, social and structural capital for sustainability. Healthcare organizations should monitor the environmental and social effects of their operations to enact their primary mission: the promotion of health.

Originality/value
The paper contributes to theory development related to the role of IC for SD in the public sector context and, in particular, in the healthcare sector where evidence is currently limited.
Vulnerable young people’s experiences of child and adolescent mental health services
Authors: Davison Jo , Zamperoni Victoria , Stain Helen J. 
Published: May 2017

Purpose
The purpose of this paper is to explore the experiences of vulnerable young people in using a local child and adolescent mental health service (CAMHS).

Design/methodology/approach
A mixed methods design was employed in which participants completed the self-report Experience of Service Questionnaire (CHI ESQ) (n=34), and a subgroup completed individual semi-structured interviews (n=17). CHI ESQ satisfaction data were also compared with a national data sample provided by the Child Outcomes Research Consortium (n=621).

Findings
Many young people appeared to have a mixed or negative experience of the CAMHS service. They strongly emphasised that feeling listened to, cared for, and supported, in addition to access and continuity of care, are key methods to enhance their experience. They also reported a lack of knowledge and stigma associated with mental health as key barriers to engagement with services more widely.

Research limitations/implications
Purposive sampling was used to recruit a carefully defined group of vulnerable young people from one school using a single CAMHS service.

Practical implications
A number of recommendations were identified that could enhance the service experience of young people.

Originality/value
There is a significant paucity of knowledge regarding young people’s views and experiences of CAMHS, especially those with high vulnerability for mental health difficulties. This exploratory study offers methods for capturing the opinions of underrepresented young people to inform future service design.