bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinico-demographic profile of burns in the Middle-East and North-Africa (MENA) region: a systematic review and meta-analysis
Authors: Elshahidi Mohamed H. 
Published: October 2024

AbstractBurn injuries remain a public health issue with risk of mortality and multiple post-burn morbidities. This article aims to systematically review and meta-analyse data about burns in the Middle-East and North-Africa (MENA) region. Methods Pubmed, Web of Science, Scopus, Global Index Medicus, Cochrane Library and Google scholar data were searched from their dates of inception till April 2024. Results One hundred and one studies were included in this review, representing data from twelve countries in the region. Data was sub-grouped into two groups: pediatric group and emergency room (ER) group. The pooled random-effect estimates were male gender 58.6% [95% CI 56.9–60.2%], accidental injury 91.1% [95% CI 85.9–94.5%], scald burn 50% [95% CI 46.7–53.4%], at home injury 79.9% [95% CI 76.7–82.8%], upper limbs involvement 51.7% [95% CI 46.8–56.6%], mean body surface area burned (BSAB) 17.2% [95% CI 14.69–19.84%], second-degree burns 56.5% [95% CI 49–63.8%], length of stay 11.18 days [95% CI 9.68–12.68%] and mortality rate 9.1% [95% CI 7.5–11%]. All included studies were of good quality. Conclusion This review shows scarcity of studies from many countries in the MENA region. These data will inform policy makers and burn specialists about the extent of this public issue in the region.
Promotion of breastfeeding in Italian Maternity Hospitals: a pre-intervention study
Authors: Davanzo Riccardo , Salvatori Guglielmo , Baldassarre Mariella , Cetin Irene , Viora Elsa , Scarpato Elena ,   on behalf of the “HPB Project” Working Group
Published: October 2024

Abstract
Background
In Italy, exclusive breastfeeding (EBF) rates at hospital discharge range unsatisfactorily between 20–97%.

Methods
In 2023, the Project for Hospital Policy on Breastfeeding (HPB) has been launched to promote breastfeeding in Italian Maternity Hospitals (MHs) as a joint initiative of the Italian Scientific Societies involved in perinatal care together with the National Midwife (FNOPO) and Nurse (FNOPI) Boards and with Vivere Onlus, a family association. The HBP Project has been designed as an uncontrolled before-after study to increase EBF rate at hospital discharge in the population of healthy, term infant with a normal weight at birth following an intervention bundle comprising: 1) Establishment of a local hospital Working Group; 2) Adoption of a hospital policy; 3) Implementation of breastfeeding monitoring; 4) Training for perinatal care professionals; 5) Enhanced implementation of the practices of skin-to-skin contact (SSC) and mother-baby rooming-in; 6) Development/Improvement of perinatal care protocols.

Results
We report the pre-intervention assessment of 89 out of the 111 enrolled MHs (80.2%) at the beginning of the Project (Time 1 or T1). Almost all MHs (96.6%) have a multi-professional Breastfeeding Working Group, while a hospital policy on breastfeeding is available only in 48.2%. Moreover, only 56.2% of the 9,777 perinatal health workers have been trained in breastfeeding. Over a 1-month period, SSC has been practiced in the delivery room by 76.9% of 6,304 term healthy newborn infants and rooming-in by 83.4% of 6,735 healthy term newborns of normal weight at birth. Over a 4-month period, 69.1% of 33,367 healthy term newborns of normal birth weight were exclusively breastfed at hospital discharge. Noticeably, EBF rate of MHs ranges from 4% up to 100%, the second quartile being 73%.

Conclusion
At T1 of the HPB Project, breastfeeding rates at hospital discharge for healthy, term infants with a normal weight at birth appear to be suboptimal among Italians MHs. Particularly, the range of EBF rates among participating centers is wide, with 50% of the MHs having EBF rate lower than 73%. Therefore, the ongoing HPB Project might represent not only an opportunity to increase the initiation of breastfeeding and to improve quality of health care in the whole study group of MHs, but possibly also to level differences between centers.
pan-Draft: automated reconstruction of species-representative metabolic models from multiple genomes
Authors: De Bernardini Nicola , Zampieri Guido , Campanaro Stefano , Zimmermann Johannes , Waschina Silvio , Treu Laura 
Published: October 2024

AbstractThe accurate reconstruction of genome-scale metabolic models (GEMs) for unculturable species poses challenges due to the incomplete and fragmented genetic information typical of metagenome-assembled genomes (MAGs). While existing tools leverage sequence homology from single genomes, this study introducespan-Draft, a pan-reactome-based approach exploiting recurrent genetic evidence to determine the solid core structure of species-level GEMs. By comparing MAGs clustered at the species-level,pan-Draftaddresses the issues due to the incompleteness and contamination of individual genomes, providing high-quality draft models and an accessory reactions catalog supporting the gapfilling step. This approach will improve our comprehension of metabolic functions of uncultured species.
Whole genome sequencing of M. tuberculosis for disease control in high-burden settings: study protocol for a cluster randomized controlled trial evaluating different community-wide intervention strategies in rural Madagascar
Authors: Ametepe Emmanuelle Sandra Adjoa , Andriamanoha Noela , Andrianomanana Fanantenana Randria , Point Floriane , Mangahasimbola Reziky Tiandraza , Dyachenko Alina , Hall Michael , Gamana Theodora Mayouya , Knoblauch Astrid M. , Razafindrasoa Yemimah Yededyah , Nimalan Arianminpathy , Behr Marcel , Durand Madeleine , Johri Mira , Iqbal Zamin , Rakotoarivelo Andry Rivo , Randremanana Rindra Vatosoa , Rakotosamimanana Niaina , Lapierre Simon Grandjean 
Published: October 2024

Abstract
Background
Retrospective and descriptive molecular epidemiology studies have shown that Mycobacterium tuberculosis whole genome sequencing can identify outbreaks and disease transmission events with higher resolution than conventional epidemiological investigations. Those studies have strengthened our understanding of genomic polymorphisms correlating with person-to-person transmission and helped resolve putative transmission clusters. To date, systematic genomic surveillance programs implemented for M. tuberculosis were only implemented in low-incidence settings. The purpose of this study is to determine whether there is an impact of routine M. tuberculosis whole genome sequencing on tuberculosis case detection in a high-incidence setting.

Methods
A cluster randomized controlled trial will be performed. Forty-eight rural village groups (or Fokontany) in the Vohibato district of Madagascar will be randomized to one of three interventions arms. Arm 1 (standard of care) involves healthcare facility-based passive case detection with smear microscopy testing. Arm 2 (best practice) consists of active case finding and Xpert MTB/RIF Ultra PCR testing followed by household contact investigations. Arm 3 (novel intervention) includes the same interventions as arm 2, with addition of sputum culture and M. tuberculosis whole genome sequencing for all newly diagnosed cases. In arm 3, molecular suggested putative outbreaks are investigated, and additional TB suspects are appropriately tested. The intervention observational period will be 2 years. The primary outcome will be the number of detected cases/100,000/year in each arm after 1 year of intervention.

Discussion
This study is designed to determine whether there is an impact of prospective whole genome sequencing-based molecular typing on tuberculosis case detection in high-incidence settings. Investigating potential outbreaks and focusing active case finding in spatiotemporal settings where disease transmission is suggested by genomic typing is hypothesized to improve case detection in rural communities.

Trial registration
ClinicalTrials.gov NCT05406453. Retrospectively registered on June 6, 2022.
Spatial and temporal characterization of Aedes albopictus oviposition activity in candidate urban settings for sterile insect technique testing in La Reunion Island
Authors: Le Goff Gilbert , Damiens David , Ruttee Abdoul-Hamid , Jean Frédéric , Payet Laurent , Lebon Cyrille , Taconet Paul , Gaudillat Benjamin , Habchi-Hanriot Nausicaa , Dehecq Jean-Sébastien , Simard Frédéric , Gouagna Louis-Clément 
Published: October 2024

Abstract
Background
Understanding of mosquito spatiotemporal dynamics is central to characterize candidate field sites for the sterile insect technique (SIT) testing, and is critical to the effective implementation and evaluation of pilot sterile male release programs. Here, we present a detailed description of Aedes albopictus (Skuse) egg-laying activity over a 6-year period in urban areas identified as potential SIT testing sites on Reunion Island.

Method
Weekly entomological collections using ovitraps were carried out in residential and adjacent uninhabited habitats in two urban areas, Duparc and Bois Rouge, in the municipality of Sainte Marie, Reunion Island. Time-series data incorporating the frequency of positive ovitraps and the total number of eggs/ovitrap recorded each time at each locality during the study period from May 2013 to December 2018 were analyzed with multifaceted statistical approaches including descriptive statistics and spatiotemporal analyses incorporating the role of climatic factors on overall ovitrap productivity.

Results
During the ovitrap survey, the proportion of egg-positive ovitraps differed among study sites (χ2 = 50.21, df = 2, P < 0.001), being relatively lower in Duparc (89.5%) than in Bois-Rouges (95.3%) and the adjacent buffer zone (91.2%). Within each neighborhood, Ae. albopictus egg abundance varied by month in a roughly seasonal pattern marked by a single peak occurring more regularly February each year, a decline at the onset of the austral winter in July, followed by a period of lower ovitrap productivity in August and September. Fluctuation in both positivity rate and eggs densities per ovitraps were related to annual and seasonal variations in local temperature and rainfall (P < 0.001 in all cases). The spatial analysis also captured substantial between- and within-habitats heterogeneity, whereby the overall ovitrap productivity was higher in residential areas than in the buffer zone.

Conclusions
Collectively, these results reveal that the distribution of Ae. albopictus oviposition activity is shaped by local habitat heterogeneity and seasonal climatic factors. Overall, this study provides baseline insights into the reproductive dynamics of Ae. albopictus, which would assist in planning locally tailored SIT interventions, while addressing concerns related to focal areas of high egg-laying intensity and potential immigration of females from natural areas.
Autophagy and autophagic cell death in sepsis: friend or foe?
Authors: Iba Toshiaki , Helms Julie , Maier Cheryl L. , Ferrer Ricard , Levy Jerrold H. 
Published: October 2024

AbstractIn sepsis, inflammation, and nutrient deficiencies endanger cellular homeostasis and survival. Autophagy is primarily a mechanism of cellular survival under fasting conditions. However, autophagy-dependent cell death, known as autophagic cell death, is proinflammatory and can exacerbate sepsis. Autophagy also regulates various types of non-inflammatory and inflammatory cell deaths. Non-inflammatory apoptosis tends to suppress inflammation, however, inflammatory necroptosis, pyroptosis, ferroptosis, and autophagic cell death lead to the release of inflammatory cytokines and damage-associated molecular patterns (DAMPs) and amplify inflammation. The selection of cell death mechanisms is complex and often involves a mixture of various styles. Similarly, protective autophagy and lethal autophagy may be triggered simultaneously in cells. How cells balance the regulatory mechanisms of these processes is an area of interest that is still under investigation. Therapies aimed at modulating autophagy are considered promising. Enhancing autophagy helps clear and recycle damaged organelles and reduce the burden of inflammatory processes while inhibiting excessive autophagy, which could prevent autophagic cell death. In this review, we introduce recent advances in research and the complex regulatory system of autophagy in sepsis.
Real-time volume rendering for three-dimensional fetal ultrasound using volumetric photon mapping
Authors: Zou Jing , Qin Jing 
Published: October 2024

AbstractThree-dimensional (3D) fetal ultrasound has been widely used in prenatal examinations. Realistic and real-time volumetric ultrasound volume rendering can enhance the effectiveness of diagnoses and assist obstetricians and pregnant mothers in communicating. However, this remains a challenging task because (1) there is a large amount of speckle noise in ultrasound images and (2) ultrasound images usually have low contrasts, making it difficult to distinguish different tissues and organs. However, traditional local-illumination-based methods do not achieve satisfactory results. This real-time requirement makes the task increasingly challenging. This study presents a novel real-time volume-rendering method equipped with a global illumination model for 3D fetal ultrasound visualization. This method can render direct illumination and indirect illumination separately by calculating single scattering and multiple scattering radiances, respectively. The indirect illumination effect was simulated using volumetric photon mapping. Calculating each photon’s brightness is proposed using a novel screen-space destiny estimation to avoid complicated storage structures and accelerate computation. This study proposes a high dynamic range approach to address the issue of fetal skin with a dynamic range exceeding that of the display device. Experiments show that our technology, compared to conventional methodologies, can generate realistic rendering results with far more depth information.
Incidence of symptomatic discoid meniscus in Korea: epidemiologic big data analysis from HIRA database
Authors: Kim Jin Seong , Kim Jung Hoon , Choi Moon Young , Ha Jeong Ku , Baek Seung Hun , Chung Kyu Sung 
Published: October 2024

Abstract
Background
There is a lack of evidence of the diagnosis and treatment-related epidemiological studies of symptomatic discoid meniscus. This study analyzed the national epidemiological data for discoid meniscus in South Korea.

Methods
From 2011 to 2019, data related to the diagnosis and procedure codes of discoid meniscus were obtained from the Korean Health Insurance Review and Assessment Service database. All patients encoded as discoid meniscus were included. Data were extracted and further analyzed as follows: (1) the total number and the incidence (cases per 100,000) of discoid meniscus diagnosis per year, (2) sex distribution, (3) age distribution, (4) discoid meniscus ratio (total discoid meniscus coding per total meniscus injury coding), and (5) surgical procedures after discoid meniscus injury.

Results
The total number of discoid meniscus diagnosed was 4576 in 2011 and increased to 6639 in 2019, representing a 45.1% increase. The incidence was 9.5 in 2011 and increased to 13.0 in 2019. Concerning sex, discoid meniscus was more common in females (55%) than in males (45%) over the study period. Regarding age, the peak age of discoid meniscus in 2011 was “under 19,” whereas in 2019, the peak age was observed in the 50s. The discoid meniscus ratio range was 2.12–2.60% from 2011 to 2019. The total number of meniscectomy increased by 20% from 2000 in 2011 to 2475 in 2014. However, the total number of meniscus repairs was 318 in 2011 and increased to 502 in 2019, indicating an increase of 58%.

Conclusions
The total number and incidence of symptomatic discoid as well as the discoid meniscus ratio and the incidence of total discoid meniscus repair steadily increased from 2011 to 2019. The number of meniscus repair procedures increased more rapidly than that of meniscectomy. The current study helps understand the epidemiology of symptomatic discoid meniscus, its prevention, and cost-saving measures in South Korea.
The battle against dental caries: defeating biofilm formed by bacterial isolates using vanillin and plant essential oils: in vitro and ex vivo approaches
Authors: Ahmed Manar M. , Yousef Nehal E. , Askoura Momen , Yahya Galal , El-Ganiny Amira M. 
Published: October 2024

Abstract
Background
Infections caused by biofilm-forming bacteria have significantly linked to dental plaque and caries. The aim of this study is to assess efficacy of some natural compounds in inhibition and eradication of biofilm formed by bacterial isolates from dental infections.

Results
Bacterial isolates were recovered from dental plaque/caries and identified using standard microbiological tests and 16S rDNA sequencing. The isolated bacterial strains include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Klebsiella pneumoniae, and Escherichia coli. The antibiotic susceptibility was determined by disk diffusion method and revealed that the majority of isolates showed high antibiotic resistance, and 61% of isolates were found to be multidrug resistant. The biofilm formation capacity of isolates was investigated using microtiter plate assay. Among the 77 bacterial isolates, seventeen showed moderate biofilm formation capacity, twenty-two showed near-moderate, thirty-four had weak biofilm-forming capacity, and four were non-biofilm producers. The antibiofilm activity of tested compounds (rose and jasmine oils, propolis, vanillin, and vinegar) was evaluated against isolates with highest biofilm-forming capacity. The in vitro antibiofilm ability of tested substances were investigated alone or in combination with each other to evaluate their ability to prevent biofilm formation or destroy preformed single-/multispecies biofilms. Finally, antibiofilm ability of tested combination was evaluated ex vivo on natural teeth. Our results showed that vanillin in combination with rose or jasmine oils showed promising biofilm inhibition and biofilm eradication activities in both the in vitro and ex vivo models.

Conclusions
Dental plaque and caries can be successfully prevented using combination of vanillin with rose or jasmine oils, and these compounds can be incorporated in new anticaries dental formulations.
Should face-to-face in-person therapy be preserved for some clients with anxiety? Evaluation of Anxiety UK's psychological therapy services before and during the COVID-19 pandemic
Authors: Paton Lewis W. , Bee Penny , Bosanquet Kate , Bower Peter , Fell Jason , Gellatly Judith , Lidbetter Nicky , Lukoseviciute Beatrice , McMillan Dean , Smithson Dave , Tiffin Paul A. 
Published: October 2024

Background
The COVID-19 pandemic initiated a mass switch to psychological therapy being delivered remotely, including at Anxiety UK, a national mental health charity. Understanding the impact of this forced switch could raise implications for the provision of psychological therapies going forwards.

Aims
To understand whether the forced switch to remote therapy had any impact on outcomes, and if certain groups should continue to be routinely offered certain delivery modalities in future.

Method
Data were available for 2323 individuals who accessed Anxiety UK services between January 2019 and October 2021. Demographic data, baseline and discharge anxiety and depression symptoms, and mode of therapy delivery were available.
Regression models were built to model (a) the mode of therapy delivery received pre-pandemic using logistic regression, and (b) outcomes pre- and post-pandemic onset within demographic groups.

Results
No statistically significant changes in baseline anxiety symptoms, demographics or outcomes were observed before and after the onset of the COVID-19 pandemic.
Pre-pandemic, males were more likely to receive online video therapy than telephone therapy (Relative Risk Ratio (RRR) 1.42, [1.01, 1.99]), while older clients were less likely to receive online video therapy (RRR 0.98, [0.97, 0.99]). However, no differences in outcomes were observed post-pandemic onset within these groups, with only the number of sessions of therapy being a significant predictor of outcomes.

Conclusions
Anxiety UK services remained effective throughout the pandemic. We observed no evidence that any demographic group had worse outcomes following the forced switch to remote therapy.
Suicide among post-Arabellion refugees in Germany
Authors: Le Nensy Thu Ha , Genuneit Jon , Brennecke Gerald , von Polier Georg , White Lars , Radeloff Daniel 
Published: October 2024

Background
Although immigrants are considered to be vulnerable to mental illness, there is limited knowledge regarding their suicide mortality.

Aims
To investigate standardised mortality ratios (SMR) for suicide among the largest immigrant populations in Germany before and after the refugee movement of 2015.

Method
Data on immigrants and the general population in Germany between 2000 and 2020 were provided by the scientific section of the Federal Statistical Office. SMR with 95% confidence intervals were calculated by indirect standardisation for gender, age and calendar year for the pre-2015 and post-2015 time interval, first for all the immigrant populations studied and second for the Syrian, Afghan and Iraqi populations separately.

Results
Immigrants from the countries studied showed a lower suicide risk compared with the German reference population (SMR = 0.38, 95% CI = 0.35–0.41). No differences in SMR were found between pre- and post-2015 time intervals, in either the aggregate data for all populations or the data for Syrian, Afghan and Iraqi populations. Post-2015, Afghan immigrants (SMR = 0.68, 95% CI = 0.54–0.83) showed a higher SMR than Syrians (SMR = 0.30, 95% CI = 0.25–0.36) or Iraqis (SMR = 0.37, 95% CI = 0.26–0.48).

Conclusions
Despite the many and varied stresses associated with flight, comparison of the pre- and post-2015 time intervals showed that the suicide risk of the populations studied did not change and was considerably lower than that of the German reference population. We attribute this to lower suicide rates in the countries of origin but also to flight-related selection processes that favour more resilient individuals.