bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic myelomonocytic leukemia: 2016 update on diagnosis, risk stratification, and management
Authors: Patnaik Mrinal M. , Tefferi Ayalew 
Published: May 2016

Chronic myelomonocytic leukemia (CMML) is a clonal hematopoietic stem cell disorder characterized by overlapping features of myelodysplastic syndromes and myeloproliferative neoplasms. Diagnosis is based on the presence of persistent (>3 months) peripheral blood monocytosis (>1 × 109/L), along with bone marrow dysplasia. Clonal cytogenetic abnormalities occur in ∼20–30% of patients, while >90% have gene mutations. Mutations involving TET2 (∼60%), SRSF2 (∼50%), ASXL1 (∼40%), and RAS (∼30%) are frequent; with only ASXL1 mutations negatively impacting overall survival. Two molecularly integrated, CMML‐specific prognostic models include; the Groupe Français des Myélodysplasies (GFM) and the Molecular Mayo Model (MMM). The GFM model segregates patients into 3 groups based on: age >65 years, WBC >15 × 109/L, anemia, platelets <100 × 109/L, and ASXL1 mutation status, with respective median survivals of 56 (low), 27.4 (intermediate), and 9.2 (high) months. The MMM is based on ASXL1 mutational status, absolute monocyte count >10 × 109/L, hemoglobin <10 g/dL, platelets <100 × 109/L and circulating immature myeloid cells. This model stratifies patients into four groups; high (≥3 risk factors), intermediate‐2 (2 risk factors), intermediate‐1 (1 risk factor) and low (no risk factors), with median survivals of 16, 31, 59, and 97 months, respectively. Hypomethylating agents such as 5‐azacitidine and decitabine are commonly used, with overall response rates of ∼30–40% and complete remission rates of ∼7–17%. Allogeneic stem cell transplant is the only potentially curative option, but is associated with significant morbidity and mortality. Individualized therapy, including epigenetic modifiers and small molecule inhibitors, are exciting prospects. Am. J. Hematol. 91:632–642, 2016. © 2016 Wiley Periodicals, Inc.
Workplace psychosocial and organizational factors for neck pain in workers in the United States
Authors: Yang Haiou , Hitchcock Edward , Haldeman Scott , Swanson Naomi , Lu Ming‐Lun , Choi BongKyoo , Nakata Akinori , Baker Dean 
Published: May 2016

BackgroundNeck pain is a prevalent musculoskeletal condition among workers in the United States. This study explores a set of workplace psychosocial and organization‐related factors for neck pain.MethodsData used for this study come from the 2010 National Health Interview Survey which provides a representative sample of the US population. To account for the complex sampling design, the Taylor linearized variance estimation method was used. Logistic regression models were constructed to measure the associations.ResultsThis study demonstrated significant associations between neck pain and a set of workplace risk factors, including work‐family imbalance, exposure to a hostile work environment and job insecurity, non‐standard work arrangements, multiple jobs, and long work hours.ConclusionWorkers with neck pain may benefit from intervention programs that address issues related to these workplace risk factors. Future studies exploring both psychosocial risk factors and physical risk factors with a longitudinal design will be important. Am. J. Ind. Med. 59:549–560, 2016. © 2016 Wiley Periodicals, Inc.
Highly Enantioselective Iridium‐Catalyzed Hydrogenation of Cyclic Enamides
Authors: Salomó Ernest , Orgué Sílvia , Riera Antoni , Verdaguer Xavier 
Published: May 2016

AbstractThe MaxPHOX–Ir catalyst system provided the highest selectivity ever reported for the reduction of cyclic enamides derived from α‐ and β‐tetralones. This result indicates that iridium catalysts are also proficient in reducing alkenes bearing metal‐coordinating groups. In the present system, selectivity was pressure‐dependent: In most cases, a decrease in the H2 pressure to 3 bar resulted in an increase in enantioselectivity. Moreover, the process can be carried out in environmentally friendly solvents, such as methanol and ethyl acetate, with no loss of selectivity.
A Disposable Planar Paper‐Based Potentiometric Ion‐Sensing Platform
Authors: Hu Jinbo , Stein Andreas , Bühlmann Philippe 
Published: May 2016

AbstractIon‐selective electrodes (ISEs) are widely used tools for fast and accurate ion sensing. Herein their design is simplified by embedding a potentiometric cell into paper, complete with an ISE, a reference electrode, and a paper‐based microfluidic sample zone that offer the full function of a conventional ISE setup. The disposable planar paper‐based ion‐sensing platform is suitable for low‐cost point‐of‐care and in‐field testing applications. The design is symmetrical and each interfacial potential within the cell is well defined and reproducible, so that the response of the device can be theoretically predicted. For a demonstration of clinical applications, paper‐based Cl− and K+ sensors are fabricated with highly reproducible and linear responses towards different concentrations of analyte ions in aqueous and biological samples. The single‐use devices can be fabricated by a scalable method, do not need any pretreatment prior to use, and only require a sample volume of 20 μL.
A morphological study on the migration and selective localization of graphene in the PLA/PMMA blends
Authors: Paydayesh Azin , Arefazar Ahmad , Jalaliarani Azam 
Published: May 2016

ABSTRACTNano‐filled polymer blends offer the opportunity to obtain materials with fine‐tuned properties. In this work, the dispersion and localization behavior of graphene nanoplate (GNP) and graphene oxide (GO) in solution mixed blends of polylactic acid (PLA) and polymethyl methacrylate (PMMA) were investigated. The blends were prepared by using different mixing sequences to investigate the effect of kinetics parameters and surface chemistry of filler as well as thermodynamics affinity on the localization of fillers. Field Emission Scanning Electron Microscopy (FESEM) and Rheometric Mechanical Spectroscopy (RMS) were employed. In addition, graphene materials were compared by Fourier transform infrared and Raman spectroscopy as well as elemental analysis characterization. Results showed that depending on the mixing sequence, the GNPs were localized in the both phases and interface through migration to reach thermodynamics equilibrium. However, GO localization was significantly affected by the mixing sequence due to better interaction with the polymer phases. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43799.
Investigation of acetone, butanol and carbon dioxide as new breath biomarkers for convenient and noninvasive diagnosis of obstructive sleep apnea syndrome
Authors: Bayrakli Ismail , Öztürk Önder , Akman Hatice 
Published: May 2016

AbstractThe objective of the present study was to investigate whether analysis of carbon dioxide, acetone and/or butanol present in human breath can be used as a simple and noninvasive diagnosis method for obstructive sleep apnea syndrome (OSAS). For this purpose, overnight changes in the concentrations of these breath molecules were measured before and after sleep in 10 patients who underwent polysomnography and were diagnosed with OSAS, and were compared with the levels of these biomarkers determined after sleep in 10 healthy subjects. The concentrations of exhaled carbon dioxide were measured using external cavity laser‐based off‐axis cavity enhanced absorption spectroscopy, whereas the levels of exhaled acetone and butanol were determined using thermal desorption gas chromatography mass spectrometry. We observed no significant changes in the levels of exhaled acetone and carbon dioxide in OSAS patients after sleep compared with pre‐sleep values and compared with those in healthy control subjects. However, for the first time, to our knowledge, analyses of expired air showed an increased concentration of butanol after sleep compared with that before sleep and compared with that in healthy subjects. These results suggest that butanol can be established as a potential biomarker to enable the convenient and noninvasive diagnosis of OSAS in the future.
Authentication of Gentiana straminea Maxim. and its substitutes based on chemical profiling of iridoids using liquid chromatography with mass spectrometry
Authors: Wu Jinrong , Zhao Zhili , Wu Lihong , Wang Zhengtao 
Published: May 2016

AbstractA liquid chromatography–electrospray ionization–ion‐trap mass spectrometry (MSn) method was established and applied for authentication of Gentiana straminea from the four substitutes, G. tibetica, G. lhassica, G. waltonii and G. robusta, based on chemical profiling of the principal iridoid glucosides aided by a quadrupole time‐of‐flight mass spectrometry. The fragmentation pathways of the three representative iridoid glucosides, loganic acid, gentiopicroside and sweroside, were investigated by MSn analysis in negative ion mode, which assisted the characterization of analogs detected in the chromatographic profiling of the tested Gentiana species. In total, 25 iridoids were identified or tentatively characterized from G. straminea and four substitutes, in which 7‐O‐(4′′‐O‐glucosyl)coumaroyl‐loganic acid and 7‐O‐coumaroyl‐loganic acid are diagnostic in G. straminea and can serve as the proposed chemical markers to discriminate it from morphologically similar substitutes.
Cortical folding of the preterm brain: a longitudinal analysis of extremely preterm born neonates using spectral matching
Authors: Orasanu Eliza , Melbourne Andrew , Cardoso Manuel Jorge , Lomabert Herve , Kendall Giles S. , Robertson Nicola J. , Marlow Neil , Ourselin Sebastien 
Published: May 2016

AbstractIntroductionInfants born extremely preterm (<28 weeks of gestation) are at risk of significant neurodevelopmental sequelae. In these infants birth coincides with a period of rapid brain growth and development, when the brain is also vulnerable to a range of insults. Mapping these changes is crucial for identifying potential biomarkers to predict early impairment.MethodsIn this study we use surface‐based spectral matching techniques to find an intrasubject longitudinal surface correspondence between the white–grey matter boundary at 30 and 40 weeks equivalent gestational age in nine extremely preterm born infants.ResultsUsing the resulting surface correspondence, we identified regions that undergo more cortical folding of the white–grey matter boundary during the preterm period by looking at changes in well‐known curvature measures. We performed Hotelling T2 statistics to evaluate the significance of our findings.DiscussionThe prefrontal and temporal lobes exhibit most development during the preterm period, especially in the left hemisphere. Such correspondences are a promising result as longitudinal measurements of change in cortical folding could provide insightful information about the mechanical properties of the underlying tissue and may be useful in inferring changes during growth and development in this vulnerable period.
From Nano to Macro through Hierarchical Self‐Assembly: The DNA Paradigm
Authors: Pfeifer Wolfgang , Saccà Barbara 
Published: May 2016

AbstractFrom atoms to molecules and bio‐macromolecules, from organelles to cells, tissues, to the whole living system, nature shows us that the formation of complex systems with emergent properties originates from the hierarchical self‐assembly of single components in guided bottom‐up processes. By using DNA as a fundamental building block with well‐known self‐recognition properties, scientists have developed design rules and physical‐chemical approaches for the fully programmable construction of highly organized structures with nanosized features. This review highlights the basic principles of hierarchical self‐assembly in terms of type and number of distinguishable components and their interaction energies. Such general concepts are then applied to DNA‐based systems. After a brief overview of the strategies used until now for the construction of individual DNA units, such as DNA tile motifs and origami structures, their self‐association into assemblies of higher order is discussed. Particular emphasis is given to the forces involved in the self‐assembly process, understanding and rational combination of which might help to coordinate the single elements of hierarchical structures both in space and time, thus advancing our efforts towards the creation of devices that mimic the complexity and functionality of natural systems.
DOCK8 deficiency in six Iranian patients
Authors: Saghafi Shiva , Pourpak Zahra , Nussbaumer Franziska , Fazlollahi Mohammad Reza , Houshmand Massoud , Hamidieh Amir Ali , Bemanian Mohammad Hassan , Nabavi Mohammad , Parvaneh Nima , Grimbacher Bodo , Moin Mostafa , Glocker Cristina 
Published: May 2016

Key Clinical MessageDOCK8 deficiency is a rare autosomal recessive combined immunodeficiency with high IgE level, eosinophilia, severe eczema, extensive cutaneous viral, and respiratory bacterial infections, mostly in populations with higher prevalence of consanguinity. Molecular diagnosis of this gene is a useful approach for early diagnosis and timely HSCT due to deleterious consequences.
Giant splenic artery aneurysm rupture
Authors: Robaldo Alessandro , Gramondo Fabrizio , Beccaria Fabio , Colotto Patrizio 
Published: May 2016

Key Clinical MessageLittle exists on treatment for SAAs rupture which may require a fast bleeding control because of the hemodynamic instability and a large perisplenic/peritoneal hematoma. This case shows the use of endovascular clamping and midline laparotomy to perform the splenectomy because of the severe hypotension and the dimension of the lesion.
Green Regio‐ and Enantioselective Aminolysis Catalyzed by Graphite and Graphene Oxide under Solvent‐Free Conditions
Authors: Acocella Maria Rosaria , D'Urso Luciana , Maggio Mario , Guerra Gaetano 
Published: May 2016

AbstractThe ring‐opening reactions of epoxides with amines were efficiently and regioselectively catalyzed by high‐surface‐area graphite and graphene oxide under metal‐free and solvent‐free conditions. For epoxides without aryl groups, catalytic activity was observed only for graphene oxide, and hence, the activity must have been due to its acidic groups. For styrene oxide, instead, graphite and graphene oxide exhibited rather similar catalytic activities, and hence, the activity was mainly due to activation of the electrophilic epoxide by π‐stacking interactions with the graphitic π system. The described aminolysis procedure is green and cheap because the catalyst can be recovered and recycled without loss of efficiency. Moreover, these heterogeneous catalysts exert high stereoselective control in the presence of nonracemic epoxides and provide chiral β‐amino alcohols with enantiomeric excess values up to 99 %.
ChemInform Abstract: Oscillations and Bistability in the Cu(II)‐Catalyzed Reaction Between H2O2 and KSCN.
Authors: ORBAN M. 
Published: May 2016

AbstractThe reaction between H2O2 and KSCN catalyzed by CuSO4 exhibits three different types of bistability dependent on the flow rate in a continuous flow stirred tank reactor at 25 °C. Two steady states and one oscillatory state, which also appears in batch configuration, are involved.
Simulation of high‐temperature steam‐only gasification of woody biomass with dry‐sorption CO2 capture
Authors: Mostafavi Ehsan , Pauls Jennifer H. , Lim C. Jim , Mahinpey Nader 
Published: May 2016

AbstractOver the last few decades, research on the abatement of carbon dioxide (CO2) gas has gained momentum, due to its increasing atmospheric levels. This study investigated high‐temperature steam‐only gasification of woody biomass for the production of high‐purity hydrogen integrated with CO2 capture in a moving‐bed gasifier. Extensive process modelling and simulation were performed using the superior solid handling features of the Aspen Plus process simulator software. After validating the model with experimental data from a demonstration plant available in the open literature, a reversible carbonation‐calcination reaction of calcium oxide (CaO) with CO2 was added to the system. Sensitivity analyses were conducted to verify the predictive accuracy of the model. The effects of steam‐to‐carbon (S/C) ratio on the resulting gas composition were thoroughly studied to delineate the complex process of gasification. Beyond the mitigation of CO2 emissions, the introduction of a CaO‐based sorbent in the process simulation significantly enhanced hydrogen production by simultaneously promoting the forward water‐gas shift reaction and reducing tars through increased tar‐cracking reactions. The results show that hydrogen of a higher purity was produced with the inclusion of dry‐sorption CO2 capture in the gasification process. Moreover, the addition of the sorbent increased the higher heating values (HHV) by 3 times and improved the cold gas efficiency by 34 %.
Play in juvenile mink: litter effects, stability over time, and motivational heterogeneity
Authors: Ahloy Dallaire Jamie , Mason Georgia J. 
Published: May 2016

AbstractMink are potentially ideal for investigating the functions of play: deleterious effects of early social isolation suggest a crucial developmental role for play; and huge numbers of highly playful juvenile subjects can be studied on farms. We collected descriptive data on 186 pairs from 93 litters, half provided with play‐eliciting environmental enrichment objects in their home cages, to test three hypotheses: (1) play frequency is subject to litter effects; (2) relative playfulness is stable over time; (3) play sub‐types share a single, common motivational basis. We found weak litter effects that were driven by stronger litter effects on general activity, and weakly stable individual differences in both total and rough‐and‐tumble play. Experimentally increasing object play did not inhibit rough‐and‐tumble play, showing these sub‐types are not motivational substitutes. Frequencies of these sub‐types were also uncorrelated, and changed differently with time of day and age, further supporting this conclusion.
Large‐for‐gestational‐age (LGA) neonate predicts a 2.5‐fold increased odds of neonatal hypoglycaemia in women with type 1 diabetes
Authors: Yamamoto Jennifer M. , Kallas‐Koeman Melissa M. , Butalia Sonia , Lodha Abhay K. , Donovan Lois E. 
Published: May 2016

AbstractObjectiveThe objective of the study is to assess the impact of maternal glycaemic control and large‐for‐gestational‐age (LGA) infant size on the risk of developing neonatal hypoglycaemia in offspring of women with type 1 diabetes and to determine possible predictors of neonatal hypoglycaemia and LGA.Research methods and designThis retrospective cohort study evaluated pregnancies in 161 women with type 1 diabetes mellitus at a large urban centre between 2006 and 2010. Mean trimester A1c values were categorized into five groups. Multiple logistic regression analyses were used to examine predictors of neonatal hypoglycaemia and large‐for‐gestational‐age (LGA).ResultsHypoglycaemia occurred in 36.6% of neonates. There was not a linear association between trimester specific A1c and LGA. After adjusting for maternal age, body mass index (BMI), smoking and premature delivery, neonatal hypoglycaemia was not linearly associated with A1c in the first, second or third trimesters. LGA was the only significant predictor for neonatal hypoglycaemia (OR, 95% CI 2.51 [1.10, 5.70]) in logistic regression analysis that adjusted for glycaemic control, maternal age, smoking, prematurity and BMI. An elevated third trimester A1c increased the odds of LGA (1.81 [1.03, 3.18]) after adjustment for smoking, parity and maternal BMI.ConclusionsLarge‐for‐gestational‐age imparts a 2.5‐fold increased odds of hypoglycaemia in neonates of women with type 1 diabetes and may be a better predictor of neonatal hypoglycaemia than maternal glycaemic control. Our data suggest that LGA neonates of women with type 1 diabetes should prompt increased surveillance for neonatal hypoglycaemia and that the presence of optimum maternal glycaemic control should not reduce this surveillance. Copyright © 2016 John Wiley & Sons, Ltd.
Intraspecific diversity buffers the inhibitory effects of soil biota
Authors: Luo Wenbo , Callaway Ragan M. , Atwater Daniel Z. 
Published: May 2016

AbstractPlant community productivity can increase with increasing intraspecific genotypic diversity. Previous studies have attributed the genetic diversity–productivity pattern to differential resource use among genotypes, as many studies have found for species. But here we ask whether suppression of productivity at low intraspecific diversity by soil biota might also drive a positive diversity–productivity relationship. In a previous study, we manipulated genetic diversity by varying the number of Pseudoroegneria accessions growing together in common garden plots, and used soil from that experiment to evaluate soil feedbacks. The total biomass of P. spicata plants grown in unsterilized soil increased with accession richness, specifically when comparing soil that had contained plants from 3 accessions to soil that had contained plants from either 8 or 12 population accessions. Furthermore, soil from high‐richness (8 or 12‐accession) plots drove neutral feedbacks, whereas soil in the 3‐accession plots (3) drove negative feedbacks. However, within each level of richness, there was no relationship between relative yield and feedback. Our results suggest that soil biota might play an integral role in the emerging understanding of the relationship between intraspecific diversity and ecosystem productivity.
SEM and ECIS Investigation of Cells Cultured on Nanopillar Modified Interdigitated Impedance Electrodes for Analysis of Cell Growth and Cytotoxicity of Potential Anticancer Drugs
Authors: Messina Walter , Fitzgerald Michelle , Moore Eric 
Published: May 2016

AbstractCell‐based biosensors treat living cells as sensing elements and are able to detect the functional information of biologically active analytes. Monitoring cytotoxicity with high sensitivity, rapidity and at low cost is of great interest in the fields of clinical diagnostics, environmental monitoring, food safety and security. This research investigates the behaviour of different cell types on nanostructured architectures. The development of cell‐based assays using bioimpedance devices has the potential of screening anti‐cancer drugs; these have a potential impact for developing new techniques and tools for the analysis of cells in the bio‐pharma industry. Gold impedance electrodes have been successfully fabricated for impedance measurement on cells cultured on the electrode surface which was modified with gold nanopillars with a height of 60 nm and 150 nm diameter in a highly ordered layout thanks to the e‐beam lithography technique. This article investigates the effects on the sensitivity achieved with the ECIS (Electric Cell‐substrate Impedance Spectroscopy) measurements while monitoring the cytotoxicity of two different drugs (Antrodia Camphorata extract and Nicotine) on different cell lines (HeLa, A549 and BALBc 3T3) cultured on the nanostructured devices. The change of morphology of cells growing on the nanostructured electrodes was also investigated through SEM imaging.
Electrochemical Biosensor for Naphthalene Acetic Acid in Fruits and Vegetables Based on Lipid Films with Incorporated Auxin‐binding Protein Receptor Using Graphene Electrodes
Authors: Bratakou Spyridoula , Nikoleli Georgia‐Paraskevi , Siontorou Christina G. , Karapetis Stephanos , Nikolelis Dimitrios P. , Tzamtzis Nikolaos 
Published: May 2016

AbstractThe present work describes a miniaturized potentiometric naphthalene acetic acid (NAA) sensor on graphene nanosheets with incorporated lipid films. Auxin‐binding protein 1 receptor immobilized on the stabilized lipid films provided adequate selectivity for detection over a wide range of hormone concentrations, fast response time of ca. 5 min, and detection limit of 10 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 56 mV/decade of hormone concentration. The reliability of the biosensor was successfully evaluated using a wide range of NAA‐spiked fruits and vegetables.