bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of two freezing methods and two cryopreservation media on post‐thaw quality of stallion spermatozoa
Authors: Macedo S , Bliebernicht M , Carvalheira J , Costa A , Ribeiro F , Rocha A 
Published: January 2018

ContentsGlycerol‐based extenders are widely utilized for freezing equine semen, but media combining methylformamide may better preserve sperm motility and mitochondrial function. Semen is cryopreserved utilizing either a Styrofoam box filled with liquid nitrogen or an automatic freezer. The objective of this experiment was to compare the post‐thaw characteristics of the same ejaculates cryopreserved in a Styrofoam box or in an automatic freezer, utilizing a glycerol‐based extender (Gent) and an extender that combines methylformamide and glycerol (BotuCrio®). For that, one ejaculate from 30 stallions collected in two different centres was used. For data analysis, a mixed linear model with laboratory, medium and freezing method and respective interactions as fixed effects was used. Stallion was taken into account as a random effect. There was no influence (p > .05) of laboratory, while stallion effect was marked. Semen frozen in BotuCrio® in the automatic freezer had higher (p < .001) VCL than semen cryopreserved in Gent using the Styrofoam box. VCL was also higher (p = .068) for semen frozen in BotuCrio® in the Styrofoam box than for semen cryopreserved in Gent using the same method. The difference between percentage of sperm with intact plasma membrane frozen in Gent using the Styrofoam box (44.43% ± 2.44%) compared to spermatozoa cryopreserved in BotuCrio® using the same method (40.78% ± 2.42%) approached significance (p = .0507). The percentage of sperm with intact acrosome membrane was higher (p < .05) in semen frozen in BotuCrio® (79.08% ± 1.79%) than semen frozen in Gent (75.15% ± 1.80%). A higher (p = .0125) percentage (32.24% ± 2.18%) of semen extended in Gent and cryopreserved in the Styrofoam box had high mitochondrial membrane potential than semen frozen in BotuCrio® using the same method (26.02% ± 2.15%). Fertility studies are warranted to assess whether differences found have any effect on the fertility of inseminated mares.
Effects of the supplementation with an high‐polyphenols extra‐virgin olive oil on kinetic sperm features and seminal plasma oxidative status in healthy dogs
Authors: Tufarelli V , Rizzo A , Lacalandra GM , Guaricci AC , Laudadio V , Valentini L 
Published: January 2018

ContentsThe aim of this work was to evaluate the effects of the supplementation of two extra‐virgin olive oils (EVOO) having different polyphenols content, on canine spermatozoa kinetic parameters and seminal plasma oxidative status. The study was conducted on 12 clinically healthy dogs of different breeds (2–7 years, 5–48 kg of body weight) divided into two groups: an experimental group supplemented with EVOO (Coratina cultivar) high in polyphenols (H‐P) and a control group fed EVOO (Cima di Bitonto cultivar) low in polyphenols (L‐P). The oil was daily administered per os (1 ml/3 kg BW) before meal. Semen collection was made twice at 15 days distance (D01 and D02) and then at 30 (D30), 60 (D60) and 90 (D90) days. Semen concentration and kinetic parameters were measured using computer‐assisted sperm analysis (CASA) system to evaluate: sperm total count, sperm motile (MOT%), progressive motility (PROGR%) and its fractions, straight‐line velocity (VSL, μm/s), curvilinear velocity (VCL, μm/s), average path velocity (VAP, μm/s), amplitude of lateral head displacement (ALH, μm), beat cross frequency (BCF, Hz), straightness (STR%) and linearity (LIN%). On seminal plasma, reactive oxygen species (ROS) and biological antioxidant potential (BAP) were tested. From findings, no differences were found for sperm MOT, VSL, VCL, VAP, ALH, BCF, STR, LIN and BAP. A gradual enhancement of PROGR% was observed in H‐P group (p < .01). The ROS levels were higher in dogs H‐P compared to the other group (p < .05). In conclusion, our results highlight the positive effects of EVOO polyphenols on sperm PROGR% in healthy dogs.
‘Our Hielandmen’: Scots in Court Entertainments at home and abroad 1507–1616
Authors: Mickel Lesley 
Published: January 2018

AbstractThis article pays attention to the ways in which Highland identity was coopted and constructed through Renaissance court entertainments both at home and further afield in Europe. Analysis of the Tournament of the Wild Knight and Black Lady staged by James IV (1507/8) reveals the tropes of wildness and blackness to underpin a particular representation of Highland identity that is appropriated and adapted by the Stewart Crown. These associations are shown to be enduring, and championed by the Scottish Crown to negate a long‐established negative discourse of Northerness, albeit these pejorative resonances continue to resonate and are eventually displaced onto depiction of Irish national identity in court culture. Pitscottie's description of the temporary hunting lodge constructed by the Earl of Atholl to host James V and the papal ambassador (1529), and Mary Stuart's festival at Stirling Castle (1566) to celebrate the christening of her son, show the Scottish Crown's deliberate evocation of the Highland characteristics of wildness to proclaim indigenous Stoic and martial virtues justifying Scottish imperial ambition to the North and South of the border.
Mitigating Litigating: An Examination of Psychosocial Impacts of Compensation Processes Associated with the 2010 BP Deepwater Horizon Oil Spill
Authors: Ritchie Liesel A. , Gill Duane A. , Long Michael A. 
Published: January 2018

AbstractDuring the past four decades, a number of social science scholars have conceptualized technological disasters as a social problem. More specifically, research in this arena has identified individual and collective stress as a secondary trauma of processes intended to provide compensation and economic relief from disasters in general and, more specifically, technological disasters. Based on data from a 2013 household telephone survey of 1,216 residents of coastal Alabama, this article examines the relationship between psychosocial stress and compensation processes related to the 2010 BP Deepwater Horizon oil spill. We examine involvement with claims, settlement, and litigation activities; vulnerability and exposure to the spill; ties to resources; resource loss and gain; perceptions of risk and recreancy; and intrusive stress and avoidance behaviors as measured by the impact of event scale. Regression analysis reveals that the strongest contributors to intrusive stress were being part of the compensation process, resource loss, concerns about air quality, and income. Although being involved with compensation processes was a significant predictor of avoidance behaviors, the strongest contributors to avoidance behaviors were resource loss, air quality concern, income, being male, minority status, and community attachment. Beliefs that the compensation process was as distressing as the oil spill also significantly contributed to intrusive stress and avoidance behaviors. This research represents a step toward filling a gap in empirical evidence regarding the extent to which protracted compensation processes exacerbate adverse psychosocial impacts of disasters and hinder community recovery.
A method of evaluating facial pores using optical 2D images and analysis of age‐dependent changes in facial pores in Koreans
Authors: Jang S. I. , Kim E. J. , Lee H. K. 
Published: January 2018

AbstractBackgroundEnlarged facial pores and changes in pore area are of concern for cosmetic reasons. To evaluate pores, measuring tools based on 3D methodology are used. Yet, these methods are limited by their measuring ranges. In this study, we performed pore analysis by measuring the whole face using 2D optical images. We further sought to understand how the pores of Korean women change with age.Materials and MethodsOne hundred sixteen Korean female subjects aged 20‐60 years were recruited for this study. Facial images were taken using the VISIA‐CR® adjusted light source. Images were processed using Image‐Pro Plus 9.2. Statistical significance was assumed when P < .05.ResultsThe pore area, as indicated by pixel count, gradually increased in patients through their 40s, but decreased through their 50s and 60s. Facial pores generally exhibited directionality through the patients’ 30s, but this isotropic feature was more prominent in their 50s. Pore elongation increased stepwise. The first increase occurred during the transition from patients’ 30s to their 40s and the second increase occurred during the transition from patients’ 50s to their 60s. This indicated that the pores deformed from a circular shape to a long elliptic shape over time.ConclusionA new evaluation method using 2D optical images facilitates the analysis of pore distribution and elongation throughout the entire cheek. This is an improvement over an analysis of pores over a narrow region of interest.
Erythema‐directed digital photography for the enhanced evaluation of topical treatments for acne vulgaris
Authors: Micali G. , Dall'Oglio F. , Tedeschi A. , Lacarrubba F. 
Published: January 2018

AbstractBackgroundErythema‐directed digital photography is a novel method for evaluating the efficacy and tolerability of topical acne treatments. Here, we describe three case reports in which erythema‐directed digital photography was used to evaluate acne before and after up to 12 weeks of treatment with clindamycin 1%/tretinoin 0.025% (Clin‐RA).Materials and methodsErythema‐directed digital photography was used to evaluate acne in three patients with mild‐to‐moderate facial acne, two of whom had refused to continue previous topical acne treatment (benzoyl peroxide 5% and clindamycin 1%/benzoyl peroxide 5%) due to persistent irritation. Acne lesions and erythema were evaluated using standard clinical photography and erythema‐directed digital photography (VISIA‐CR™ system) before and after 8‐12 weeks of treatment with Clin‐RA.ResultsErythema‐directed digital photography revealed background erythema from previous topical acne treatments that was not evident from standard clinical photographs and allowed a better visualization of both inflammatory and non‐inflammatory lesions. In all patients, there was a clear improvement in background erythema and a reduction in acne lesions following treatment with Clin‐RA.ConclusionThis study has demonstrated for the first time that erythema‐directed digital photography can enhance the evaluation of the efficacy and tolerability of topical acne treatments. These cases show that Clin‐RA was associated with improved efficacy and tolerability vs previous treatments with topical monotherapy (benzoyl peroxide 5%) or a topical fixed‐dose combination (clindamycin 1%/benzoyl peroxide 5%).
Unraveling lipid metabolism in maize with time‐resolved multi‐omics data
Authors: de Abreu e Lima Francisco , Li Kun , Wen Weiwei , Yan Jianbing , Nikoloski Zoran , Willmitzer Lothar , Brotman Yariv 
Published: January 2018

SummaryMaize is the cereal crop with the highest production worldwide, and its oil is a key energy resource. Improving the quantity and quality of maize oil requires a better understanding of lipid metabolism. To predict the function of maize genes involved in lipid biosynthesis, we assembled transcriptomic and lipidomic data sets from leaves of B73 and the high‐oil line By804 in two distinct time‐series experiments. The integrative analysis based on high‐dimensional regularized regression yielded lipid–transcript associations indirectly validated by Gene Ontology and promoter motif enrichment analyses. The co‐localization of lipid‐transcript associations using the genetic mapping of lipid traits in leaves and seedlings of a B73 × By804 recombinant inbred line population uncovered 323 genes involved in the metabolism of phospholipids, galactolipids, sulfolipids and glycerolipids. The resulting association network further supported the involvement of 50 gene candidates in modulating levels of representatives from multiple acyl‐lipid classes. Therefore, the proposed approach provides high‐confidence candidates for experimental testing in maize and model plant species.
The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids [Rosa L. sect. Caninae (DC.) Ser.] exhibiting non‐symmetrical meiosis
Authors: Herklotz Veit , Kovařík Aleš , Lunerová Jana , Lippitsch Susan , Groth Marco , Ritz Christiane M. 
Published: January 2018

SummaryDogroses represent an exceptional system for studying the effects of genome doubling and hybridization: their asymmetrical meiosis enables recombination in bi‐parentally inherited chromosomes but prevents it in maternally inherited ones. We employed fluorescent in situ hybridization, genome skimming, amplicon sequencing of genomic and cDNA as well as conventional cloning of nuclear ribosomal DNA in two phylogenetically distinct pentaploid (2n = 5x = 35) species, Rosa canina and Rosa inodora, and their naturally occurring reciprocal hybrids, Rosa dumalis (5x) and Rosa agrestis (5x, 6x). Both progenitor species differed in composition, meiotic behaviour and expression of rDNA loci: R. canina (five 18S and 5–8 5S loci) was dominated by the Canina ribotypes, but R. inodora (four 18S loci and 7–8 5S loci) by the Rubiginosa ribotype. The co‐localized 5S/18S loci occurred on either bivalent‐forming (R. canina) or univalent‐forming (R. inodora) chromosomes. Ribosomal DNA loci were additively inherited; however, the Canina ribotypes were dominantly expressed, even in genotypes with relatively low copy number of these genes. Moreover, we observed rDNA homogenization towards the paternally transmitted Canina ribotype in 6x R. agrestis. The here‐observed variation in arrangement and composition of rDNA types between R. canina and R. inodora suggests the involvement of different genomes in bivalent formation. This results supports the hypothesis that the asymmetrical meiosis arose at least twice by independent ancient hybridization events.
Comparison of diode laser trans‐scleral cyclophotocoagulation versus implantation of a 350‐mm2Baerveldt glaucoma drainage device for the treatment of glaucoma in dogs (a retrospective study: 2010‐2016)
Authors: Graham Kathleen L. , Hall Evelyn J.S. , Caraguel Charles , White Andrew , Billson Francis A. , Billson F. Mark 
Published: January 2018

AbstractObjectiveTo compare outcomes following trans‐scleral cyclophotocoagulation (TSCP) and 350‐mm2Baerveldt implantation in the treatment of canine refractory glaucoma.DesignRetrospective case study.Case selectionClient owned dogs undergoing surgical treatment of glaucoma within a veterinary referral hospital.ProceduresEighty‐six glaucoma surgeries were performed on 83 eyes (69 dogs) diagnosed with primary or secondary glaucoma. Medical records were retrieved, and baseline data, surgery, medications, intraocular pressures (IOPs), vision, and complications were extracted.ResultsFifty‐four eyes (44 dogs) were treated withTSCPand placement of an anterior chamber suture shunt; 28 eyes (24 dogs) were implanted with a Baerveldt glaucoma drainage device (GDD); and four eyes (4 dogs) underwentGDDimplantation after failure ofTSCPto manageIOP. FollowingTSCP,IOPcontrol (<20 mmHg) and vision retention occurred in 81.5% and 42.6%, respectively, for 16.1 ± 1.36 months. FollowingGDDimplantation, 71.4% maintainedIOP<20 mmHg and 69.6% maintained vision for 11.0 ± 0.94 months.IOPcontrol without loss of vision was more likely following Baerveldt implantation (17/28; 60.7%) thanTSCP(19/54; 35.2%) (P =0.027). One eye had functional vision restored followingGDDplacement.IOPcontrol without adjunctive medications was more likely following Baerveldt implantation (P= 0.02).ConclusionsIn this study, eyes treated with BaerveldtGDDimplantation were more likely to maintainIOPcontrol and retain vision compared to eyes treated withTSCPand placement of an anterior chamber suture shunt. Lack of formal randomization, inconsistencies in surgical techniques andTSCPprotocols, and potential unmeasured confounders must be considered when extrapolating from this retrospective study.
The Novel Fungal Cyp51 Inhibitor VT-1598 Is Efficacious in Experimental Models of Central Nervous System Coccidioidomycosis Caused by Coccidioides posadasii and Coccidioides immitis
Authors: Wiederhold Nathan P. , Shubitz Lisa F. , Najvar Laura K. , Jaramillo Rosie , Olivo Marcos , Catano Gabriel , Trinh Hien T. , Yates Christopher M. , Schotzinger Robert J. , Garvey Edward P. , Patterson Thomas F. 
Published: January 2018

ABSTRACT

Coccidioidal meningitis can cause significant morbidity, and lifelong antifungal therapy is often required. VT-1598 is a fungus-specific Cyp51 inhibitor that has potent
in vitro
activity against
Coccidioides
species. We evaluated the
in vivo
efficacy of VT-1598 in murine models of central nervous system coccidioidomycosis caused by
C. posadasii
and
C. immitis
. Infection was introduced via intracranial inoculation, and therapy began 48 h postinoculation. Oral treatments consisted of vehicle control, VT-1598, and positive controls of fluconazole in the
C. immitis
study and VT-1161 in the
C. posadasii
study. Treatment continued for 7 and 14 days in the fungal-burden and survival studies, respectively. Fungal burden was assessed in brain tissue collected 24 to 48 h posttreatment in the fungal-burden studies, on the days the mice succumbed to infection, or at prespecified endpoints in the survival studies. VT-1598 plasma concentrations were also measured in the
C. posadasii
study. VT-1598 resulted in significant improvements in survival in mice infected with either species. In addition, the fungal burden was significantly reduced in the fungal-burden studies. Plasma concentrations 48 h after dosing stopped remained above the VT-1598 MIC against the
C. posadasii
isolate, although levels were undetectable in the survival study after a 4-week washout. Whereas fungal burden remained suppressed after a 2-week washout in the
C. immitis
model, a higher fungal burden was observed in the survival arm of the
C. posadasii
model. This
in vivo
efficacy supports human studies to establish the utility of VT-1598 for the treatment of coccidioidomycosis.
Draft Genome Sequences of 11
Salmonella enterica
Serovar Typhimurium Strains Isolated from Human Systemic and Nonsystemic Sites in Brazil
Authors: Panzenhagen Pedro Henrique N. , Paul Narayan C. , Conte Junior Carlos A. , Costa Renata G. , Rodrigues Dália P. , Shah Devendra H. 
Published: January 2018

ABSTRACT

Salmonella enterica
serovar Typhimurium strains isolated from systemic sites outside sub-Saharan Africa have been rarely sequenced. Here, we report the draft genome sequences of
S
. Typhimurium sequence type 19 (ST19) (
n
= 9), ST1649 (
n
= 1), and ST313 (
n
= 1) strains isolated from human systemic (e.g., blood) and nonsystemic (e.g., stool and wounds) sites in Brazil.
Draft Genome Sequence of
Monaibacterium marinum
C7
T
, Isolated from Seawater from the Menai Straits, Wales, United Kingdom
Authors: Chernikova Tatyana N. , Kyrpides Nikos , Bargiela Rafael , Woyke Tanja , Shapiro Nicole , Whitman William B. , Golyshin Peter N. 
Published: January 2018

ABSTRACT

Here, we report the draft genome sequence of
Monaibacterium marinum
C7
T
, a strain that represents a new member of the
Roseobacter
clade of the family
Rhodobacteraceae
(
Alphaproteobacteria
). The genome size of
Monaibacterium marinum
C7
T
is 3.7 Mb (3,734,267 bp), with a G+C content of 58.86%.
Draft Genome Sequences of 42
Helicobacter pylori
Isolates from Rural Regions of South India
Authors: Shetty Vignesh , Lamichhane Binit , Chua Eng-Guan , Ballal Mamatha , Tay Chin-Yen 
Published: January 2018

ABSTRACT

Helicobacter pylori
is a successful human gastric pathogen that is associated with the development of gastric cancer. The draft genome sequences of 42
H. pylori
clinical strains isolated from South Indian rural populations will provide further insights into the evolution and genetic makeup of Indian
H. pylori
strains.
Complete Genome Sequence of Cluster J Mycobacteriophage Superphikiman
Authors: Pradhan Pratik , Nako Sprikena , Tran Trinh , Aluri Lavanya S. , Anandarajan Dharman , Betini Niteesha , Bhatt Shivangi D. , Chengalvala Swetha , Cox Nicole E. , Delvadia Bela P. , Desai Aishwary S. , Devaney Andrew M. , Doyle Brenna K. , Edgerton Arden O. , Erlich Matthew C. , Fitzpatrick Kevin C. , Gajjar Esha A. , Ganguly Anjali , Gill Ramnik S. , Good Pauline M. , Gupta Nishtha , Haddad Leila M. , Han Esther J. , Jain Shelby , Jiang Andrew , Jurgielewicz Andrew D. , Kainth Devneet K. , Karam Jawhara M. , Kodavatiganti Mallika , Kriete Sinja J. , MacDonald Catherine E. , Maret Josh P. , Mathew Ashley E. , Natrajan Maanasa , Nishu Nusrat M. , Patel Nirali , Patel Pooja D. , Patel Shivani , Patra Kaustav , Rai Karima K. , Sarkar Arghyadeep , Shah Priyanka , Tata Ravi K. , Tawfik Andrew H. , Thuremella Bhavya T. , Toma Justina , Veera Shika , Vemulapalli Vamsee K. , Vidas Trevor V. , Vieira Katy S. , Vijayakumar Gayathri , Walor Tru A. , White Clara R. , Wong Brianna M. , Zhao Shu L. , Bollivar David W. , McDonald Matthew T. , Dalia Ritu R. , Smith Kevin P. W. , Little Joy L. , Gurney Susan M. R. 
Published: January 2018

ABSTRACT
Mycobacteriophage Superphikiman is a cluster J bacteriophage which was isolated from soil collected in Philadelphia, PA. Superphikiman has a 109,799-bp genome with 239 predicted genes, including 2 tRNA genes.
Improved Draft Genome Sequence of
Microcystis aeruginosa
NIES-298, a Microcystin-Producing Cyanobacterium from Lake Kasumigaura, Japan
Authors: Yamaguchi Haruyo , Suzuki Shigekatsu , Kawachi Masanobu 
Published: January 2018

ABSTRACT

Microcystis aeruginosa
is a globally well-known bloom-forming cyanobacterium. An improved draft whole-genome sequence of
M. aeruginosa
NIES-298, which is a microcystin-producing strain isolated from Lake Kasumigaura, Japan, is published here. The genome comprises approximately 5.0 Mbp, with an average G+C content of 42.6% and 4,537 predicted protein-coding genes.
Draft Genome Sequences of Eight
Staphylococcus aureus
Strains Isolated during Foodborne Outbreaks
Authors: Abaev Igor , Skryabin Yury , Kislichkina Angelina , Bogun Alexandr , Korobova Olga , Dyatlov Ivan 
Published: January 2018

ABSTRACT

We report here the draft genome sequences of eight
Staphylococcus aureus
strains isolated during three large food poisoning outbreaks in the Russian Federation. The strains were collected from clinical specimens and various foodstuff samples.
Draft Genome Sequence of a Trimethylamine-Producing
Staphylococcus
Isolate from Blood of a Coronary Atherosclerotic Heart Disease Patient
Authors: Yu Xue , Kang Ying-Qian , Ji Fusui 
Published: January 2018

ABSTRACT

Trimethylamine (TMA), which is produced by various bacteria, is associated with heart disease, but little information about the production of TMA in blood is available. We present here the genome sequence of a multidrug-resistant strain,
Staphylococcus epidermidis
bTMA-013, with a TMA synthesis pathway, which was isolated from the blood of a coronary atherosclerotic heart disease patient.
Complete Genome Sequence of
Xanthomonas citri
pv.
anacardii
Strain IBSBF2579 from Brazil
Authors: Silva Junior Wilson José , Farias Antonio Roberto Gomes , Lima Nelson Bernardi , Benko-Iseppon Ana Maria , Aburjaile Flávia , Balbino Valdir Queiroz , Falcão Raul Maia , Leitão Paiva Júnior Sérgio de Sa , Sousa-Paula Lucas Christian , Mariano Rosa Lima Ramos , Souza Elineide Barbosa , Gama Marco Aurélio Siqueira 
Published: January 2018

ABSTRACT

The bacterium
Xanthomonas citri
pv.
anacardii
is the agent of angular leaf spot of the cashew tree (
Anacardium occidentale
L.). The complete genome sequencing of the strain IBSBF2579 was done on an Illumina HiSeq 2500 platform. The
de novo
assembly of the
X. citri
pv.
anacardii
strain IBSBF2579 genome yielded 133 contigs, with a size of 5,329,247 bp and a G+C content of 64.03%. The prediction was performed by GeneMarkS and the automatic annotation by Rapid Annotations using Subsystems Technology (RAST), with 4,406 identified genes.