bluuebunny/crossref_metadata_2025_split huggingface dataset:
LC-MS guided isolation of three pairs of enantiomeric alkaloids from Macleaya cordata and their enantioseparations, antiproliferative activity, apoptosis-inducing property
Authors: Sai Chunmei , Li Dahong , Li Shengge , Han Tong , Guo Yongzhi , Li Zhanlin , Hua Huiming 
Published: November 2017

Abstract(±)-Macleayins F–H (1–3), three pairs of new enantiomeric alkaloid dimers, along with four known alkaloids (4–7) as their plausible biogenetic precursors, were isolated from the aerial parts of Macleaya cordata. Compounds 1–3 were obtained under the guidance of LC-MS investigation, and their structures were elucidated by analysis of the 1D and 2D NMR spectroscopic data. The racemic mixtures were successfully separated by chiral HPLC, and the absolute configurations of enantiomers were determined by electronic circular dichroism (ECD) spectroscopy. Compounds 1–7 showed antiproliferative activity against HL-60 with IC50 values of 1.34–41.30 μM, especially compounds 1–2 exhibited the best inhibitory activity against HL-60 cell lines. In addition, the preliminary mechanism investigation for compound 2 using Annexin V/7-AAD double-staining assay, DAPI staining assay and JC-1 staining method, indicated that 2 inhibited cancer cell proliferation potentially through inducing apoptosis via the mitochondria-related pathway and arrested cell cycle of HL-60 cells at S phase.
A non-smooth tumor margin on preoperative imaging assesses microvascular invasion of hepatocellular carcinoma: A systematic review and meta-analysis
Authors: Hu HangTong , Zheng Qiao , Huang Yang , Huang Xiao Wen , Lai Zhi Cheng , Liu JingYa , Xie XiaoYan , Feng Shi Ting , Wang Wei , Lu Ming De 
Published: November 2017

AbstractMicrovascular invasion (MVI) is rarely diagnosed preoperatively in hepatocellular carcinoma (HCC). The aim of this meta-analysis is to assess the diagnostic power of a non-smooth tumor margin on preoperative imaging for MVI. We performed a literature search using the PubMed, Embase and Cochrane Library databases, and 11 studies were included involving 618 MVI-positive cases and 1030 MVI-negative cases. Considerable heterogeneity was found, and was indicated to be attributable to the mean patient ages in the included studies. In subgroups of studies with a mean patient age older than 60 years and studies with computed tomography (CT) as the imaging method (as opposed to magnetic resonance imaging (MRI)), heterogeneity was low, and the diagnostic odds ratio (DOR) of the single two-dimensional imaging feature for MVI was 21.30 (95% CI [12.52, 36.23]) and 28.78 (95% CI [13.92, 59.36]), respectively; this power was equivalent to or greater than that of certain multivariable-based scoring systems. In conclusion, a non-smooth tumor margin on preoperative imaging is of great value for MVI assessment and should be considered for inclusion in future scoring systems.
Validation of Self-reported Smoking with Urinary Cotinine Levels and Influence of Second-hand Smoke among Conscripts
Authors: Chiu Yu-Lung , Huang Shu-Jia , Lai Ching-Huang , Huang Chung-Chi , Jiang Shiang-Huei , Li Shan-Ru , Hwang Shu-Ling , Lin Fu-Gong , Tzeng Ya-Mei , Kao Senyeong 
Published: November 2017

AbstractAccurate identification of smoking behaviour is crucial to monitor the smoking rate. This study used urinary cotinine (UC) as a biomarker to verify the effectiveness of self-reported smoking behaviour among conscripts during recruit training. The influence of second-hand smoke (SHS) on the UC concentration was also analysed. A cross-sectional study was conducted from July 2014 to December 2014. The participants comprised a total of 621 military service and basic military training conscripts. A self-administered questionnaire survey and a urine test were performed to verify the participants’ smoking behaviour. The UC concentration of 100 ng/mL was adopted as the baseline to identify smokers. A high level of consistency was observed between the conscripts’ self-reported results and the results validated by the UC concentrations (the overall kappa coefficient was 0.918). Moreover, the overall sensitivity and specificity were 92.9% and 98.1%, respectively. The sensitivity for the military service conscripts was significantly lower than that for the basic military training conscripts (86.1% vs. 97.5%, P-value = 0.002). For the self-reported nonsmokers among the military service conscripts, SHS exposure was related to their UC concentrations. The method of self-reporting through a questionnaire survey can serve as a tool to assess conscripts’ smoking behaviour.
Absorption and Extinction Cross Sections and Photon Streamlines in the Optical Near-field
Authors: Striebel Moritz , Wrachtrup Jӧrg , Gerhardt Ilja 
Published: November 2017

AbstractThe optical interaction of light and matter is modeled as an oscillating dipole in a plane wave electromagnetic field. We analyze absorption, scattering and extinction for this system by the energy flow, visualized as streamlines of the Poynting vector. Depending on the dissipative damping of the oscillator, a part of the streamlines ends up in the dipole. Based on a graphical investigation of the streamlines, this represents the absorption cross section, and forms a far-field absorption aperture. In the near-field of the oscillator, a modification of the aperture is observed. As in the case for a linear dipole, we model the energy flow and derive the effective absorption apertures for an oscillator with a circular dipole characteristics – such as an atom in free space.
Selectivity and ligand-based molecular modeling of an odorant-binding protein from the leaf beetle Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) in relation to habitat-related volatiles
Authors: Wang Yinliang , Jin Yincan , Chen Qi , Wen Ming , Zhao Hanbo , Duan Hongxia , Ren Bingzhong 
Published: November 2017

AbstractIn this study, the most abundant and antenna-specific odorant-binding protein (OBP) of the elm pest A. quadriimpressum, AquaOBP4, was expressed and purified. The selectivity of AquaOBP4 was investigated by screening against a panel of 40 habitat-relevant compounds. Based on the obtained results, a homologous model of AquaOBP4 was established. This model indicated that AquaOBP4 is highly homologous to DmelOBP LUSH and includes two main binding sites. A docking analysis showed that four of five active ligands bound at Site 1, whereas the other ligand was situated at Site 2. Furthermore, new ligands were docked in the model, and the results of fluorescence-based binding assays of these compounds were highly consistent with the binding conformation and binding affinity predicted by our model. Additionally, three binding odorants derived from elm leaves elicited a strong electroantennogram response and exerted a significant attractive effect on adult A. quadriimpressum. All of the results showed that AquaOBP4 is likely linked to the foraging behavior of A. quadriimpressum. This study provides a new reliable tool for future large-scale compound screenings and revealed several functional chemicals that might aid the development of a better pest management approach for A. quadriimpressum.
Decreased oxygen saturation levels in neonates with transposition of great arteries: Impact on appearance of cerebral veins in susceptibility-weighted imaging
Authors: Verma Rajeev Kumar , Keller Desislava , Grunt Sebastian , Bigi Sandra , Weisstanner Christian , Wiest Roland , Gralla Jan , Hutter Damian , Wagner Bendicht 
Published: November 2017

AbstractPurpose of this study was to investigate a potential correlation between the pattern of cerebral veins (CV) on susceptibility-weighted imaging (SWI) and blood oxygen saturation, as well as preoperative brain injury, in neonates with transposition of the great arteries (TGA). Eleven neonates with TGA underwent MRI preoperatively, including SWI, T1- and T2-weighted scans. Images were retrospectively evaluated and appearance of CV was graded from 0 (normal appearance) to 3 (severe prominent appearance). White matter injuries (WMI) and strokes were analysed. Results were correlated with preductal arterial oxygen saturation. As findings one subject showed a normal CV appearance (grade 0) whereas 10 showed pathological prominent CV (grades 1–3); median 2. Mean oxygen saturation ranged between 67.5% and 89.0% (median 81.0%). CV grade and mean oxygen saturation correlated significantly (p = 0.011). WMI were absent in 5 cases, mild in 4, and moderate in 2 cases. We conclude, that SWI has the potential to be used to estimate the current hypoxic burden on brain tissue in TGA newborns by assessing the prominence of the CV.
Effects of microgravity on osteoblast mitochondria: a proteomic and metabolomics profile
Authors: Michaletti Anna , Gioia Magda , Tarantino Umberto , Zolla Lello 
Published: November 2017

AbstractThe response of human primary osteoblasts exposed to simulated microgravity has been investigated and analysis of metabolomic and proteomic profiles demonstrated a prominent dysregulation of mitochondrion homeostasis. Gravitational unloading treatment induced a decrease in mitochondrial proteins, mainly affecting efficiency of the respiratory chain. Metabolomic analysis revealed that microgravity influenced several metabolic pathways; stimulating glycolysis and the pentose phosphate pathways, while the Krebs cycle was interrupted at succinate-fumarate transformation. Interestingly, proteomic analysis revealed that Complex II of the mitochondrial respiratory chain, which catalyses the biotransformation of this step, was under-represented by 50%. Accordingly, down-regulation of quinones 9 and 10 was measured. Complex III resulted in up-regulation by 60%, while Complex IV was down-regulated by 14%, accompanied by a reduction in proton transport synthesis of ATP. Finally, microgravity treatment induced an oxidative stress response, indicated by significant decreases in oxidised glutathione and antioxidant enzymes. Decrease in malate dehydrogenase induced a reverse in the malate-aspartate shuttle, contributing to dysregulation of ATP synthesis. Beta-oxidation of fatty acids was inhibited, promoting triglyceride production along with a reduction in the glycerol shuttle. Taken together, our findings suggest that microgravity may suppress bone cell functions, impairing mitochondrial energy potential and the energy state of the cell.
Low perceptual sensitivity to altered video speed in viewing a soccer match
Authors: de’Sperati Claudio , Baud Bovy Gabriel 
Published: November 2017

AbstractWhen watching videos, our sense of reality is continuously challenged. How much can a fundamental dimension of experience such as visual flow be modified before breaking the perception of real time? Here we found a remarkable indifference to speed manipulations applied to a popular video content, a soccer match. In a condition that mimicked real-life TV watching, none of 100 naïve observers spontaneously noticed speed alterations up/down to 12%, even when asked to report motion anomalies, and showed very low sensitivity to video speed changes (Just Noticeable Difference, JND = 18%). When tested with a constant-stimuli speed discrimination task, JND was still high, though much reduced (9%). The presence of the original voice-over with compensation for pitch did not affect perceptual performance. Thus, our results document a rather broad tolerance to speed manipulations in video viewing, even under attentive scrutiny. This finding may have important implications. For example, it can validate video compression strategies based on sub-threshold temporal squeezing. This way, a soccer match can last only 80 min and still be perceived as natural. More generally, knowing the boundaries of natural speed perception may help to optimize the flow of artificial visual stimuli which increasingly surround us.
Deglacial Tropical Atlantic subsurface warming links ocean circulation variability to the West African Monsoon
Authors: Schmidt Matthew W. , Chang Ping , Parker Andrew O. , Ji Link , He Feng 
Published: November 2017

AbstractMultiple lines of evidence show that cold stadials in the North Atlantic were accompanied by both reductions in Atlantic Meridional Overturning Circulation (AMOC) and collapses of the West African Monsoon (WAM). Although records of terrestrial change identify abrupt WAM variability across the deglaciation, few studies show how ocean temperatures evolved across the deglaciation. To identify the mechanism linking AMOC to the WAM, we generated a new record of subsurface temperature variability over the last 21 kyr based on Mg/Ca ratios in a sub-thermocline dwelling planktonic foraminifera in an Eastern Equatorial Atlantic (EEA) sediment core from the Niger Delta. Our subsurface temperature record shows abrupt subsurface warming during both the Younger Dryas (YD) and Heinrich Event 1. We also conducted a new transient coupled ocean-atmosphere model simulation across the YD that better resolves the western boundary current dynamics and find a strong negative correlation between AMOC strength and EEA subsurface temperatures caused by changes in ocean circulation and rainfall responses that are consistent with the observed WAM change. Our combined proxy and modeling results provide the first evidence that an oceanic teleconnection between AMOC strength and subsurface temperature in the EEA impacted the intensity of the WAM on millennial time scales.
Blastocyst Formation Rate and Transgene Expression are Associated with Gene Insertion into Safe and Non-Safe Harbors in the Cattle Genome
Authors: Ghahfarokhi Milad Khorramian , Dormiani Kianoush , Mohammadi Ali , Jafarpour Farnoosh , Nasr-Esfahani Mohammad Hossein 
Published: November 2017

AbstractIntegration target site is the most important factor in successful production of transgenic animals. However, stable expression of transgene without disturbing the function of the host genome depends on promoter methylation, transgene copy number and transcriptional activity in integration regions. Recently, new genome-editing tools have made much progress, however little attention has been paid to the identification of genomic safe harbors. The aim of the present study was to evaluate the effect of insertion site, promoter and copy number of transgene on the production of embryos from cattle fibroblast cells following somatic cell nuclear transfer (SCNT). So, three donor vectors were constructed with EGFP gene under control of different promoters. Each vector was integrated into safe and non-safe harbors in the genome using phiC31 integrase. Transgenic clones with a single copy of each vector were isolated. Each clone was analyzed to find site and frequency of integration, expression level and promoter methylation before SCNT, as well as transgene expression level and blastocyst formation rate after SCNT. The data obtained demonstrated that BF5, as a safe harbor, not only showed a stable expression, but also the rate of in vitro-produced embryos from BF5-clones are similar to that of non-transfected cells.
Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD
Authors: Shu Jiaze , Li Defu , Ouyang Haiping , Huang Junyi , Long Zhen , Liang Zhihao , Chen Yuqin , Chen Yiguan , Zheng Qiuyu , Kuang Meidan , Tang Haiyang , Wang Jian , Lu Wenju 
Published: November 2017

AbstractAnimal model of cigarette smoke (CS) –induced chronic obstructive pulmonary disease (COPD) is the primary testing methodology for drug therapies and studies on pathogenic mechanisms of disease. However, researchers have rarely run simultaneous or side-by-side tests of whole-body and nose-only CS exposure in building their mouse models of COPD. We compared and evaluated these two different methods of CS exposure, plus airway Lipopolysaccharides (LPS) inhalation, in building our COPD mouse model. Compared with the control group, CS exposed mice showed significant increased inspiratory resistance, functional residual capacity, right ventricular hypertrophy index, and total cell count in BALF. Moreover, histological staining exhibited goblet cell hyperplasia, lung inflammation, thickening of smooth muscle layer on bronchia, and lung angiogenesis in both methods of CS exposure. Our data indicated that a viable mouse model of COPD can be established by combining the results from whole-body CS exposure, nose-only CS exposure, and airway LPS inhalation testing. However, in our study, we also found that, given the same amount of particulate intake, changes in right ventricular pressure and intimal thickening of pulmonary small artery are a little more serious in nose-only CS exposure method than changes in the whole-body CS exposure method.
Post-earthquake Zika virus surge: Disaster and public health threat amid climatic conduciveness
Authors: Reina Ortiz Miguel , Le Nicole K. , Sharma Vinita , Hoare Ismael , Quizhpe Edy , Teran Enrique , Naik Eknath , Salihu Hamisu M. , Izurieta Ricardo 
Published: November 2017

AbstractA recent major earthquake (M7.8), coupled with appropriate climatic conditions, led to significant destruction in Ecuador. Temperature variations, which may be induced by anthropogenic climate change, are often associated with changes in rainfall, humidity and pressure. Temperature and humidity are associated with ecological modifications that may favour mosquito breeding. We hypothesized that the disruptive ecological changes triggered by the earthquake, in the context of appropriate climatic conditions, led to an upsurge in Zika virus (ZIKV) infections. Here we show that, after controlling for climatic and socioeconomic conditions, earthquake severity was associated with incident ZIKV cases. Pre-earthquake mean maximum monthly temperature and post-earthquake mean monthly pressure were negatively associated with ZIKV incidence rates. These results demonstrate the dynamics of post-disaster vector-borne disease transmission, in the context of conducive/favourable climatic conditions, which are relevant in a climate change-affected world where disasters may occur in largely populated areas.
Community effect of cardiomyocytes in beating rhythms is determined by stable cells
Authors: Hayashi Tatsuya , Tokihiro Tetsuji , Kurihara Hiroki , Yasuda Kenji 
Published: November 2017

AbstractThe community effect of cardiomyocytes was investigated in silico by the change in number and features of cells, as well as configurations of networks. The theoretical model was based on experimental data and accurately reproduced recently published experimental results regarding coupled cultured cardiomyocytes. We showed that the synchronised beating of two coupled cells was tuned not to the cell with a faster beating rate, but to the cell with a more stable rhythm. In a network of cardiomyocytes, a cell with low fluctuation, but not a hight frequency, became a pacemaker and stabilised the beating rhythm. Fluctuation in beating rapidly decreased with an increase in the number of cells (N), almost irrespective of the configuration of the network, and a cell comes to have natural and stable beating rhythms, even for N of approximately 10. The universality of this community effect lies in the fluctuation-dissipation theorem in statistical mechanics.
Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene induce partial oncolysis of Canine Transmissible Venereal Tumor
Authors: Bhat Aubid Hussain , Ganguly Bhaskar , Tiwari Ashok Kumar , Das Arup Kumar 
Published: November 2017

AbstractThe oncolytic effect of Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene in naturally occurring cases of Canine Transmissible Venereal Tumor (CTVT) is being reported. Dogs suffering from CTVT (N = 18) were systematically randomized into three groups viz. A, B, and C (n = 6). Animals of the groups A, B, and C received 100 µg of the ns1 gene, vp3 gene, and ns1 + vp3 gene combination, respectively, for three weeks intratumorally at weekly intervals; results were normalized against base values before commencement of therapy and after complete remission that were taken as negative and positive controls, respectively. Initiation of oncolytic gene therapy arrested the further progression of the tumor but most of the animals in the study underwent incomplete remission, indicating incomplete activity of ns1 and vp3 genes. The oncolytic effect of the treatments was in the order ns1 > vp3 > ns1 + vp3. Oncolysis was accompanied by decreased mitotic index and AgNOR count, and increased TUNEL positive cells and CD4+ lymphocyte counts. Our findings show that Canine Parvovirus ns1 may eventually find an important role as an oncolytic agent.
Plug-and-play round-robin differential phase-shift quantum key distribution
Authors: Mao Qian-Ping , Wang Le , Zhao Sheng-Mei 
Published: November 2017

AbstractThe round-robin differential-phase-shift quantum key distribution (RRDPS-QKD) protocol could provide an effective way to estimate the leakage information without monitoring the signal disturbance. Moreover, the self-compensating property of plug-and-play (P&P) setup can eliminate the variations of phase or polarization in QKD procedure. In the paper, we introduce the P&P concept into RRDPS-QKD, and propose a QKD protocol, named P&P RRDPS-QKD protocol, to make the RRDPS-QKD scheme more practical. We analyze the security, and discuss the key generation rate with infinite-intensity decoy state method. The results show that the proposed protocol is a good solution to RRDPS-QKD protocol with untrusted sources. It has a high security and its key generation rate could be as good as the protocol with trusted sources when the average input photon number N is greater than 106. In addition, the proposed protocol has a high noise tolerance in comparison with P&P BB84-QKD protocol.
Utilization of Stainless-steel Furnace Dust as an Admixture for Synthesis of Cement-based Electromagnetic Interference Shielding Composites
Authors: Fan Yong , Zhang Ling , Volski Vladimir , Vandenbosch Guy A. E. , Blanpain Bart , Guo Muxing 
Published: November 2017

AbstractElectromagnetic interference (EMI) shielding receives attention due to the increasing abundance of electronics. The Cement based material can obtain EMI shielding properties through the use of appropriate “fillers” such as carbon, metal, and ferrite. As the most important by-product of stainless steelmaking operations, through the metal droplets and ferrite that it contains, stainless-steel dust can be considered as a potential filler for EMI shielding applications. We have therefore utilized stainless-steel dust as an admixture for the synthesis of cement-based EMI shielding composites and show that it raises the EMI shielding effectiveness. In particular, a 45 mass pct of stainless-steel dust mixture of 5 mm thickness results in the enhancement of EMI shielding effectiveness to 6–9 dB as tested in the frequency range of 500 MHz–1.5 GHz.