bluuebunny/crossref_metadata_2025_split huggingface dataset:
A highly conserved sequence of the viral TAP inhibitor ICP47 is required for freezing of the peptide transport cycle
Authors: Matschulla Tony , Berry Richard , Gerke Carolin , Döring Marius , Busch Julia , Paijo Jennifer , Kalinke Ulrich , Momburg Frank , Hengel Hartmut , Halenius Anne 
Published: June 2017

AbstractThe transporter associated with antigen processing (TAP) translocates antigenic peptides into the endoplasmic reticulum (ER) lumen for loading onto MHC class I molecules. This is a key step in the control of viral infections through CD8+ T-cells. The herpes simplex virus type-1 encodes an 88 amino acid long species-specific TAP inhibitor, ICP47, that functions as a high affinity competitor for the peptide binding site on TAP. It has previously been suggested that the inhibitory function of ICP47 resides within the N-terminal region (residues 1–35). Here we show that mutation of the highly conserved50PLL52motif within the central region of ICP47 attenuates its inhibitory capacity. Taking advantage of the human cytomegalovirus-encoded TAP inhibitor US6 as a luminal sensor for conformational changes of TAP, we demonstrated that the50PLL52motif is essential for freezing of the TAP conformation. Moreover, hierarchical functional interaction sites on TAP dependent on50PLL52could be defined using a comprehensive set of human-rat TAP chimeras. This data broadens our understanding of the molecular mechanism underpinning TAP inhibition by ICP47, to include the50PLL52sequence as a stabilizer that tethers the TAP-ICP47 complex in an inward-facing conformation.
Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
Authors: Chen Hanxiang , Zhang Qishu , Qiao Lijun , Fan Xueli , Zhang Weifang , Zhao Weiming , Chen Jason J. 
Published: June 2017

AbstractThe human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for their potential role in this process. To further explore the mechanism by which E7 bypasses hypoxia-induced G1 arrest, we applied a proteomic approach and used mass spectrometry to search for proteins that are differentially expressed in E7 expressing cells under hypoxia. Among differentially expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic activities when overexpressed. We have recently demonstrated that Cdc6 was required for E7-induced re-replication. Significantly, here we showed that Cdc6 played a role in E7-mediated G1 checkpoint abrogation under hypoxic condition, and the function could possibly be independent from its role in DNA replication initiation. This study uncovered a new function of Cdc6 in regulating cell cycle progression and has important implications in HPV-associated cancers.
Dynamic Triple-Mode Sorption and Outgassing in Materials
Authors: Sharma Hom N. , Harley Stephen J. , Sun Yunwei , Glascoe Elizabeth A. 
Published: June 2017

AbstractMoisture uptake and outgassing can be detrimental to a system by altering the chemical and mechanical properties of materials within the system over time. In this work, we conducted isotherm experiments to investigate dynamic moisture sorption and desorption in markedly different materials, i.e., a polymeric material, Sylgard-184 and a ceramic aluminosilicate material, Zircar RS-1200, at different temperatures (30 °C–70 °C) by varying the water activity (0.0–0.90). Sylgard-184 showed a linear sorption and outgassing behavior with no-hysteresis over the entire temperature and water activity range considered here. Whereas, the sorption and outgassing of Zircar RS-1200 was highly non-linear with significant hysteresis, especially at higher water activities, at all temperatures considered here. The type of hysteresis suggested the presence of mesopores in Zircar RS-1200, whereas the lack of hysteresis in Sylgard-184 indicates that it has a nonporous structure. A diffusion model coupled with a dynamic, triple-mode sorption (Langmuir, Henry, and pooling modes) model employed in this study matched our experimental data very well and provides mechanistic insight into the processes. Our triple-mode sorption model was adaptive enough to (1) model these distinctly different materials and (2) predict sorption and outgassing under conditions that are distinctly different from the parameterization experiments.
Biogeography of Amazon birds: rivers limit species composition, but not areas of endemism
Authors: Oliveira Ubirajara , Vasconcelos Marcelo F. , Santos Adalberto J. 
Published: June 2017

AbstractAmazonian rivers are usually suggested as dispersal barriers, limiting biogeographic units. This is evident in a widely accepted Areas of Endemism (AoEs) hypothesis proposed for Amazonian birds. We empirically test this hypothesis based on quantitative analyses of species distribution. We compiled a database of bird species and subspecies distribution records, and used this dataset to identify AoEs through three different methods. Our results show that the currently accepted Amazonian AoEs are not consistent with areas identified, which were generally congruent among datasets and methods. Some Amazonian rivers represent limits of AoEs, but these areas are not congruent with those previously proposed. However, spatial variation in species composition is correlated with largest Amazonian rivers. Overall, the previously proposed Amazonian AoEs are not consistent with the evidence from bird distribution. However, the fact that major rivers coincide with breaks in species composition suggest they can act as dispersal barriers, though not necessarily for all bird taxa. This scenario indicates a more complex picture of the Amazonian bird distribution than previously imagined.
Paradoxical puborectalis syndrome on diffusion-weighted imaging: a retrospective study of 72 cases
Authors: Liu Guiqin , Cui Zhe , Dai Yongming , Yao Qiuying , Xu Jianrong , Wu Guangyu 
Published: June 2017

AbstractThis study aimed to evaluate the application value of diffusion-weighted imaging (DWI) for assessing paradoxical puborectalis syndrome (PPS) in patients with obstructive defecation syndrome (ODS). The medical records of 72 ODS patients who underwent magnetic resonance (MR)-DWI and MR-defecography were retrospectively reviewed. The differences in the apparent diffusion coefficients (ADCs) and the thickness of the right and left branches of the puborectalis muscles between the PPS(+) and PPS(−) groups were compared. In addition, the absolute within-patient differences between the right and left branches (ADC, thickness) were compared between the two groups. The absolute difference in ADCs (right branch - left branch) was significantly different between the two groups. Regardless of whether the ADC was acquired through single-ROI (0.10 ± 0.08 vs 0.23 ± 0.18, P = 0.000) or multi-ROI (0.16 ± 0.14 vs 0.27 ± 0.17, P = 0.009) analysis, the PPS(+) patients displayed a lower absolute ADC difference than did the PPS(−) patients. However, there was no statistically significant difference in the ADC value, thickness or the absolute difference in thickness between the two groups. These findings suggest that DWI may have value in quantitatively assessing the puborectalis muscle in ODS patients, whereas the value of puborectalis thickness in such aspect needs further study.
Low genetic diversity and potential inbreeding in an isolated population of alder buckthorn (Frangula alnus) following a founder effect
Authors: Finlay Caroline M. V. , Bradley Caroline R. , Preston S. Jane , Provan Jim 
Published: June 2017

AbstractAlder buckthorn (Frangula alnus) is one of Ireland’s rarest tree species, and in Northern Ireland the species is now restricted to a single population in Peatlands Park, Co. Armagh numberingca. 140 mature trees. Genotyping of 95% of the trees at nine nuclear microsatellite loci revealed that levels of genetic diversity within this population were generally lower than those reported from larger populations in Spain. Analysis of six chloroplast microsatellite loci revealed no variation. The level ofFISwas significantly higher than that in the Spanish populations, as well as in other populations across Europe, potentially indicating inbreeding. Spatial autocorrelation analysis indicated some evidence of fine-scale genetic structuring, most likely due to limited seed dispersal, but the overall level of differentiation between subpopulations was low, indicating high levels of gene flow, probably due to cross-pollination by bees. Our results are consistent with a gradual population expansion from a limited number of individuals. We suggest that more immediate conservation efforts might be best focused on ensuring suitable habitat for the continued recovery of this isolated population.
PH-Dependent Enantioselectivity of D-amino Acid Oxidase in Aqueous Solution
Authors: Liu Qingju , Chen Li , Zhang Zhikun , Du Bibai , Xiao Yating , Yang Kunhao , Gong Lingling , Wu Li , Li Xiangjun , He Yujian 
Published: June 2017

AbstractD-amino acid oxidases (DAAO) are stereospecific enzymes which are generally almost inactive towards L-enantiomer in neutral solution when L-, D-amino acids are supplied as substrates. In this paper, the D-amino acid oxidase can catalytic oxidize L-amino acids by modulating pH of aqueous solution. With L-Pro as substrate, the catalytic rate (kcat) and the affinity (Km) of DAAO were 6.71 s−1 and 33 mM at pH 8.0, respectively, suggesting that optimal pH condition enhanced the activity of DAAO towards L-Pro. Similar results were obtained when L-Ala (pH 9.8), L-Arg (pH 6.5), L-Phe (pH 9.0), L-Thr (pH 9.4), and L-Val (pH 8.5) were catalyzed by DAAO at various pH values. The racemization of the L-amino acids was not found by capillary electrophoresis analysis during oxidation, and quantification analysis of L-amino acids before and after catalytic reaction was performed, which confirmed that the modulation of enantioselectivity of DAAO resulted from the oxidation of L-amino acids rather than D-amino acids by changing pH. A mechanistic model was proposed to explain enhanced activity of DAAO towards L-amino acids under optimal pH condition.
Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
Authors: Amimanan Piyawan , Tavichakorntrakool Ratree , Fong-ngern Kedsarin , Sribenjalux Pipat , Lulitanond Aroonlug , Prasongwatana Vitoon , Wongkham Chaisiri , Boonsiri Patcharee , Umka Welbat Jariya , Thongboonkerd Visith 
Published: June 2017

AbstractEscherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation.
Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
Authors: Lopalco Patrizia , Stahl Julia , Annese Cosimo , Averhoff Beate , Corcelli Angela 
Published: June 2017

AbstractAcidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606﻿T﻿ and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.
The Complexity of Standing Postural Sway Associates with Future Falls in Community-Dwelling Older Adults: The MOBILIZE Boston Study
Authors: Zhou Junhong , Habtemariam Daniel , Iloputaife Ikechukwu , Lipsitz Lewis A. , Manor Brad 
Published: June 2017

AbstractStanding postural control is complex, meaning that it is dependent upon numerous inputs interacting across multiple temporal-spatial scales. Diminished physiologic complexity of postural sway has been linked to reduced ability to adapt to stressors. We hypothesized that older adults with lower postural sway complexity would experience more falls in the future. 738 adults aged ≥70 years completed the Short Physical Performance Battery test (SPPB) test and assessments of single and dual-task standing postural control. Postural sway complexity was quantified using multiscale entropy. Falls were subsequently tracked for 48 months. Negative binomial regression demonstrated that older adults with lower postural sway complexity in both single and dual-task conditions had higher future fall rate (incident rate ratio (IRR) = 0.98, p = 0.02, 95% Confidence Limits (CL) = 0.96–0.99). Notably, participants in the lowest quintile of complexity during dual-task standing suffered 48% more falls during the four-year follow-up as compared to those in the highest quintile (IRR = 1.48, p = 0.01, 95% CL = 1.09–1.99). Conversely, traditional postural sway metrics or SPPB performance did not associate with future falls. As compared to traditional metrics, the degree of multi-scale complexity contained within standing postural sway-particularly during dual task conditions- appears to be a better predictor of future falls in older adults.
Technical and economic comparison between direct current and half‐wavelength transmission systems for very long distances
Authors: Samorodov German , Kandakov Simeon , Zilberman Samuil , Krasilnikova Tatyana , Tavares Maria Cristina , Machado Camilo , Li Qingmin 
Published: June 2017

This study presents the results of technical and economic analysis of high‐voltage direct current (HVDC) transmission system and high‐voltage alternating current transmission system for bulk transmission over very long distances. The last is a point‐to‐point transmission that does not need reactive compensation and does not have intermediate substations, the half‐wavelength transmission (HWL). Special attention is paid to reliability and ways to meet the N  − 1 criteria regarding security and adequacy. Single‐circuit lines instead of double‐circuit ones are considered for economic and environmental reasons ensuring the same reliability when clearing the most frequent line‐to‐ground (or single‐pole) fault. For this, single‐circuit line with reserve phase for HWL and quadripolar single‐circuit line for HVDC are proposed. The analysed system considered Brazilian electrical system conditions: 6000 MW transmission over 2500 km. This study highlights the advantages of applying a reserve phase for HWLs and a third converter substation increase to cope with system security with minimum cost. It is shown that the single‐circuit HWL with reserve phase has an investment 30% lower than that for HVDC, resulting in a total yearly cost 15% lower.
Revision Rhinoplasty: Retrospective Chart Review Analysis of Deformities and Surgical Maneuvers in Patients with Nasal Airway Obstruction—Five Years of Experience
Authors: Daskalakis Dimitrios , Patel Kalpesh , Goudakos John 
Published: June 2017

AbstractNasal airway obstruction is one of the most frequent causes of revision rhinoplasty in patients after previous rhinoplasty procedure. Purpose of this study is to present the deformities and the surgical maneuvers conducted in revision rhinoplasty patients with functional complaint, anatomical sites at risk, and potential prophylactic maneuvers. This study is a retrospective chart review analysis of 46 consecutive revision rhinoplasty procedures in patients with nasal airway obstruction. Inclusion criteria were at least one previous rhinoplasty and nasal airway obstruction as the epicenter of patients' complaint. Thorough clinical examination to certify the obstruction was performed. Deformities noted were separated in three categories according to functional, cosmetic, and combination of functional and cosmetic implications. Surgical maneuvers conducted were reviewed. Deformities found were checked for statistically significant coexistences. The average patient age was 34.9 years. The mean number of previous septorhinoplasties was 1.33. Nasal ventilation obstruction mainly caused either by septum deviation or nasal valve dysfunction was identified in 91.3% of our patients. Surgical maneuvers conducted included placement of grafts in 89.1% of all cases, septoplasty in 76.1%, lateral wall support in 47.8%, and placement of spreader grafts in 39.1% of patients. The average preoperative Nasal Obstruction Symptom Evaluation (NOSE) score was 61 ± 15 and it improved substantially, even from month 1, postoperatively. Owing to high prevalence of nasal airway obstruction after primary or secondary rhinoplasty, we conducted the first retrospective chart review study to identify the most common deformities in revision rhinoplasty patients with nasal airway obstruction and the appropriate surgical maneuvers to address them. Septum deviation and nasal valve dysfunction were the two pillars of nasal airway obstruction in those patients.
Nontraumatic Emergency of the Spine
Authors: Belloch Elena , Gallegos Paulina , Higueras Victoria , Taberner Esther , LLopis Eva 
Published: June 2017

Nontraumatic spinal emergencies can have many different causes. Clinical symptoms may be nonspecific, and therefore radiology plays a key role in diagnosing and managing these patients. There is considerable time pressure in these situations because the development of irreversible myelopathy depends not only on the cause but also on the time elapsed between the start of compression and the decompression. To avoid structural cord damage, decompression should be done within 6 to 8 hours after the start of the compression. If patients still walk when the lesion is detected, they have a 90 to 100% chance of walking when the lesion is treated immediately.Magnetic resonance imaging is the primary method for the evaluation of spinal emergencies. An appropriate fast protocol should be used, adding some special sequences depending on the clinical scenario.In this review we use a simple anatomical approach that can be applied in an acute practical clinical setting, allowing an accurate differential diagnosis that will guide subsequent therapeutic actions. We highlight key radiologic features that will help nonspecialized radiologists make a precise diagnosis.
Decision Making: Osteoplasty, Ablation, or Combined Therapy for Spinal Metastases
Authors: Tutton Sean , Lea William 
Published: June 2017

AbstractMetastatic disease involving the spine and pelvis is common, often resulting in significant pain and disability. Several percutaneous interventions have been described, including osteoplasty, ablation, and screw fixation, that when used alone or in combination can significantly reduce pain and disability from metastatic bone disease. Although it is possible to make a significant impact in patient care with basic principles and techniques, certain advanced techniques can extend the application of percutaneous interventions while minimizing morbidity.
Neurological Involvement in Pediatric Hemolytic Uremic Syndrome: A Symptom-Oriented Analysis
Authors: Özdoğan Elif , Kaya Gülay , Özgün Nezir , Cansu Ali , Kalyoncu Mukaddes , Dilber Embiya , Şahin Sevim 
Published: June 2017

Background Neurological involvement is common in hemolytic uremic syndrome (HUS), but each neurological symptom may be due to a variety of factors.
Objective We aimed to evaluate predisposing factors to the neurological symptoms in HUS.
Materials and Methods The 10-year follow-up data on HUS patients were retrospectively analyzed. Statistical comparisons were made across subgroups based on age and neurological symptoms.
Results The neurological involvement rate was 37.5%. The female-to-male ratio increased in patients with neurological involvement (3.8 vs. 1.37). Regarding all HUS patients, hemoglobin levels were higher in patients with paresis. The rate of paresis was twofold higher in patients with a hemoglobin level above 11 g/dL (p < 0.05). In diarrhea-associated HUS patients, D-dimer and C-reactive protein levels were higher in patients with paresis, and leukocyte counts were higher in patients with seizures. Patients with altered consciousness had higher creatinine levels. The survival rate was significantly lower in patients with a reticulocyte percentage of less than 2% (50 vs. 100%).
Conclusion Results of our study indicate that neurological involvement depends on overall findings in HUS. A symptom-oriented approach, which is different from that employed in previous studies, reveals some clues to the pathogenesis and management of these patients.
Incidence of Sudden Unexpected Postnatal Collapse in the Therapeutic Hypothermia Era
Authors: Laudani Emanuela , Tubili Flavia , Calvani Maura , Bartolini Isabella , Donzelli Gianpaolo , Filippi Luca 
Published: June 2017

Objective Hypothermia is considered the treatment of choice for newborns with hypoxic-ischemic encephalopathy. Even though currently no consensus exists regarding the opportunity to extend this treatment also to newborns with sudden unexpected postnatal collapse (SUPC), this treatment is frequently adopted. The aim of the study was to evaluate whether the availability of hypothermia also for collapsed newborns in a tertiary level center may impact the estimated incidence of SUPC.
Study Design A retrospective study was performed comparing the number of newborns admitted with the diagnosis of SUPC in the tertiary referral Neonatal intensive care unit of Florence in the period before (2008–2010) and after (2011–2014) the introduction of hypothermia treatment for newborns with SUPC.
Result After introducing active cooling also to newborns with SUPC, the number of newborns with SUPC admitted to our center augmented and, consequently, SUPC incidence apparently increased from 5.3 to 15.5/100,000 live births, even though this difference did not reach statistical significance (odds ratio = 0.340; 95% confidence interval = 0.071–1.639; p = 0.179).
Conclusion The apparent increase of SUPC incidence is probably related to a more effective centralization of newborns in our referral center but suggests that the real incidence of SUPC is probably significantly higher than currently estimated.