bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak
Authors: Fu Yuxin , Yan Jiuru , Lan Lan , Zhang Huizhu , Wang Peng , Wang Yaying , Xiong Xianrong , Li Jian , He Honghong 
Published: October 2024

IntroductionThe CDO1 gene is an important gene in the taurine synthesis pathway and has been observed to have high expression in ovaries of female mammals. This study aims to explore the conservation of CDO1 gene in domestic yaks, as well as to examine the fundamental characteristics of CDO1 gene and its expression in female yaks.MethodsOvarian samples were collected from yaks in the follicular phase, luteal phase and gestation period in this experiment, and their total RNA and protein were extracted. Then Polymerase Chain Reaction (PCR) and bioinformatics online software were used to clone and analyze the CDO1 gene. The relative expression of CDO1 in yak ovaries was detected by Quantitative Real-time PCR (RT-qPCR) and Western blotting. The distribution and localization of CDO1 protein in ovary were detected by immunohistochemistry.ResultsWe have successfully cloned the coding region of CDO1 gene in yak. The results showed that the CDS region of CDO1 gene was 603 bp, encoding 200 amino acids, and was a relatively stable hydrophilic protein. CDO1 is relatively conservative in species evolution. The protein encoded by CDO1 gene does not have a signaling peptide or a transmembrane structure. It is a protein that is not involved in transmembrane transport and is mainly located in the cytoplasm. The secondary structure of the protein is dominated by the random coil. CDO1 is estimated to interact with 10 proteins. The results of RT-qPCR and Western blotting showed that the CDO1 gene exhibited the highest expression in the ovary during the luteal phase and the lowest expression in the ovary during the follicular phase (P &lt; 0.01). The results of immunohistochemistry showed that CDO1 was mainly expressed in granular cells, theca cells and lutein cells of ovarian tissue.ConclusionThese results suggest that the CDO1 gene has undergone minimal evolutionary changes during the course of animal evolution. The results provide a reference for further investigation of the function of CDO1 gene in reproduction and production in yaks.
Enhancing Bone Formation Through bFGF-Loaded Mesenchymal Stromal Cell Spheroids During Fracture Healing in Mice
Authors: Takeda Kugo , Saito Hiroki , Shoji Shintaro , Sekiguchi Hiroyuki , Matsumoto Mitsuyoshi , Ujihira Masanobu , Miyagi Masayuki , Inoue Gen , Takaso Masashi , Uchida Kentaro 
Published: October 2024

This study aimed to evaluate the osteogenic potential of mesenchymal stromal cell (MSC) spheroids combined with the basic fibroblast growth factor (bFGF) in a mouse femur fracture model. To begin, MSC spheroids were generated, and the expression of key trophic factors (bFGF Bmp2, and Vegfa) was assessed using quantitative PCR (qPCR). A binding assay confirmed the interaction between the bFGF and the spheroids’ extracellular matrix. The spheroid cultures significantly upregulated bFGF, Bmp2, and Vegfa expression compared to the monolayers (p < 0.001), and the binding assay demonstrated effective bFGF binding to the MSC spheroids. Following these in vitro assessments, the mice were divided into five groups for the in vivo study: (1) no treatment (control), (2) spheroids alone, (3) bFGF alone, (4) bFGF-loaded spheroids (bFGF-spheroids), and (5) non-viable (frozen) bFGF-loaded spheroids (bFGF-dSpheroids). Bone formation was analyzed by a micro-CT, measuring the bone volume (BV) and bone mineral content (BMC) of the mice four weeks post-fracture. A high dose of the bFGF (10 µg) significantly promoted bone formation regardless of the presence of spheroids, as evidenced by the increases in BV (bFGF, p = 0.010; bFGF-spheroids, p = 0.006; bFGF-dSpheroids, p = 0.032) and BMC (bFGF, p = 0.023; bFGF-spheroids, p = 0.004; bFGF-dSpheroids, p = 0.014), compared to the controls. In contrast, a low dose of the bFGF (1 µg) combined with the MSC spheroids significantly increased BV and BMC compared to the control (BV, p = 0.012; BMC, p = 0.015), bFGF alone (BV, p = 0.012; BMC, p = 0.008), and spheroid (BV, p < 0.001; BMC, p < 0.001) groups. A low dose of the bFGF alone did not significantly promote bone formation (p > 0.05). The non-viable (frozen) spheroids loaded with a low dose of the bFGF resulted in a higher BV and BMC compared to the spheroids alone (BV, p = 0.003; BMC, p = 0.017), though the effect was less pronounced than in the viable spheroids. These findings demonstrate the synergistic effect of the bFGF and MSC spheroids on bone regeneration. The increased expression of the BMP-2 and VEGF observed in the initial experiments, coupled with the enhanced bone formation in vivo, highlight the therapeutic potential of this combination. Future studies will aim to elucidate the underlying molecular mechanisms and assess the long-term outcomes for bone repair strategies.
PHÂN LẬP VÀ ĐÁNH GIÁ TIỀM NĂNG PROBIOTIC CÁC CHỦNG Bacillus subtilis TẠI CÁC TỈNH, THÀNH ĐỒNG BẰNG SÔNG CỬU LONG
Authors: Lý Thị Thu Lan  , Nguyễn Thị Anh Thư  , Nguyễn Thị Mỹ Hằng  , Võ Nguyễn Nghĩa  
Published: October 2024

ISOLATION AND EVALUATION OF POTENTIAL PROBIOTIC OF Bacillus subtilis IN THE MEKONG DELTA PROVINCES, VIETNAM
Tóm tắt: Thu thập 60 mẫu ruột gà từ các trại gà ở thành phố Cần Thơ, tỉnh Trà Vinh, và tỉnh An Giang, nghiên cứu ghi nhận được 22 chủng có khả năng là vi khuẩn Bacillus spp. với các đặc điểm như vi khuẩn hình que, khuẩn lạc trắng đục, tròn lồi, và là vi khuẩn gram dương. Các chủng vi khuẩn được tìm thấy (22 chủng) tiếp tục được khảo sát về khả năng di động và xin bào tử. Kết quả lựa chọn được 5/22 chủng để tiến hành chạy PCR và giải trình tự gen. Các vi khuẩn nghi ngờ được xác định bằng kĩ thuật giải mã trình tự gen 16S rRNA. Hai khuẩn tìm được là Bacillus subtilis, đặc tính probiotic của hai chủng TV12 và AG10 được khảo sát các đặc điểm bao gồm: khả năng bám dính, sức chịu đựng được trong môi trường pH và muối mật của hệ tiêu hoá, mức độ tự bám dính. Tại pH 2 và 3, hai vi khuẩn có tỉ lệ sống khá cao là 56,07% (TV12), 55,06% (AG10) và duy trì tỉ lệ sống trong suốt 180 phút khảo sát ở nồng độ muối mật 0,3%. Về mức độ tự bám dính, khả năng liên kết của hai chủng tăng gấp đôi sau 5 giờ thử thách ở nhiệt độ tiêu chuẩn.
Abstract: The study was conducted on 60 chicken intestine samples collected from chicken farms in Can Tho City, Tra Vinh Province, and An Giang Province. The results obtained a total of 22 potential Bacillus spp. with cultural characteristics such as circular colonies, fuzzy white, round, rod-shaped bacteria, and gram-positive. These bacterial strains (22 strains) were further examined for motility and spore formation capabilities. Five out of the 22 strains were selected for PCR and gene sequencing. Suspected bacteria were identified using 16S rRNA gene sequencing. After collecting the Bacillus subtilis, the probiotic properties of the two strains TV12 and AG10 were evaluated with criteria including the tolerance of acid bile salts (pH and bile salts), and aggregation ability. The study recorded that the two bacterial strains had quite high survival rates of 56.07% (TV12) and 55.06% (AG10), respectively at pH 2 and 3, and maintained survival rates during 180 minutes at 0.3% bile salt concentration. Regarding aggregation ability and high temperature doubled after 5 hours of incubation at standard temperature.
NGHIÊN CỨU TỔNG HỢP NANO BẠC MANG ĐẶC TÍNH KHÁNG KHUẨN VÀ TIỀM NĂNG ỨNG DỤNG TRONG NÔNG NGHIỆP
Authors: Nguyễn Như Khải  , Đặng Thị Oanh Yến  , Nguyễn Ngọc Thanh  , Nguyễn Nhật Nam  
Published: October 2024

FABRICATION OF SILVER NANOPARTICLES AND THEIR ANTIBACTERIAL POTENTIAL FOR AGRICULTURAL APPLICATION
Tóm tắt: Vật liệu nano ra đời và phát triển với nhiều ứng dụng trong nông nghiệp bền vững như phòng trị bệnh, xử lí môi trường canh tác, và chuẩn đoán bệnh trên cây trồng. Hệ nano bạc (AgNPs) được triển khai thiết kế và chế tạo bằng phương pháp mới mang đặc tính kháng khuẩn đem lại tiềm năng ứng dụng cao trong nông nghiệp. Trong nghiên cứu này, AgNPs được tổng hợp thông qua sự kết hợp các chất hoạt động bề mặt sinh học không ion với chất hoạt động bề mặt ion ở điều kiện 65℃ trong 1 giờ. Các đặc tính quang học và hình thái AgNPs được xác định bằng quang phổ hấp thụ UV-Vis và kính hiển vi điện tử quét (SEM). AgNPs tổng hợp được có sự phân bố đồng nhất đạt kích thước 62±2,8 nm và phổ hấp thụ UV-Vis thu nhận với đỉnh thấp thu ở ~420 nm. Đồng thời, các hạt AgNPs biểu hiện đặc tính kháng khuẩn đối với Staphylococcus aureus ở các nồng độ khảo sát 80, 8, và 0,8 ppm với vòng tròn đường kính kháng khuẩn tương ứng là 12, 10, và 8 mm. Các thử nghiệm ban đầu cho thấy hệ AgNPs tổng hợp được không gây độc cho cây, có khả năng ứng dụng trong phòng và trị bệnh cho cây trồng.
Abstract: The development of nanomaterials has led to numerous applications in sustainable agriculture including disease prevention and treatment, cultivation environment management, and crop disease diagnostics. As-prepared silver nanoparticles have been designed and synthesized using a novel method, showcasing antibacterial properties that hold significant potential for agricultural use. In this study, silver nanoparticleswere synthesized through the combination of non-ionic biological surfactants with ionic surfactants at 65℃ for 1 hour. The optical properties and morphology of silver nanoparticleswere characterized by UV-Vis absorbance and scanning electron microscope (SEM), respectively. The results show that AgNPs had a high-homogeneity of size distribution of 62±2.8 nm and a single UV-vis absorbance band was obtained at ~420 nm. Moreover, as-prepared silver nanoparticles showed antibacterial activity with Staphylococcus aureus with diameters zones of 12, 10, and 8 mm in silver nanoparticles concentrations range of 80, 8, and 0.8 ppm, respectively. Initial tests indicate that the synthesized silver nanoparticles are non-toxic to plants and have potential applications in the prevention and treatment of plant diseases.
NGHIÊN CỨU THỰC NGHIỆM CHỌN LỰA GIỐNG DƯA LÊ THÍCH HỢP CANH TÁC ĐIỀU KIỆN NGOÀI ĐỒNG Ở VỤ XUÂN HÈ TẠI TỈNH TRÀ VINH
Authors: Phan Chí Hiếu  , Sơn Thị Thanh Nga  , Phan Quốc Nam  
Published: October 2024

EXPERIMENTAL STUDY ON SELECTION OF MUSKMELON VARIETIES ADAPTED TO FIELD CULTIVATION CONDITIONS IN THE SPRING-SUMMER SEASON IN TRA VINH PROVINCE, VIETNAM
Tóm tắt: Mục tiêu nghiên cứu là lựa chọn giống dưa lê (Cucumis melo L.) thích nghi với điều kiện bất lợi ở ngoài đồng tại vùng đất phù sa phủ trên nền cát biển tại Trại Thực nghiệm Trồng trọt của Trường Đại học Trà Vinh trong vụ Xuân Hè 2022. Phương pháp thiết kế thí nghiệm theo kiểu lô sọc, 06 nghiệm thức là tổ hợp 03 giống dưa lê nhập nội F1 (Kim Hoàng Hậu, Bạch Ngọc Đường, Kim Ngọc Đường) kết hợp bón với 02 liều lượng K2O khác nhau là ̣94 K2O (kg/ha) 139 K2O (kg/ha), 03 lần lặp lại. Kết quả cho thấy cả 03 giống dưa lê thí nghiệm đều có sai khác về chiều dài lá, độ rộng lá, chiều dài thân sau 20 ngày trồng và chiều dài trái, chu vi trái, khối lượng trái. Trong đó, giống dưa lê Kim Hoàng Hậu có khối lượng quả cao nhất 1,325 kg/quả và năng suất lí thuyết 26,50 tấn/ ha. Độ Brix cao nhất 14,13% ở giống dưa lê Bạch Ngọc Đường bón công thức phân 139 K2O (kg/ha).
Abstract: The research objective is to select muskmelon varieties adapted to adverse conditions in the field in alluvial soils covered with sea sand at the Experimental Cultivation Camp of Tra Vinh University in the Spring-Summer season 2022. The experimental design method is in the style of striped batches, six treatments are a combination of three varieties of imported melon F1 (Kim Hoang Hau, Bach Ngoc Duong, Kim Ngoc Duong) combined with two different doses of K2O: 94 K2O (kg/ha) and 139 K2O (kg/ha), three repetitions. The results showed that all three experimental muskmelon varieties had differences in leaf length, leaf width, stem length after 20 days of planting and fruit length, fruit circumference, and fruit mass. Among them, Kim Hoang Hau muskmelon variety has the highest fruit weight of 1.325 kg/fruit and a theoretical yield of 26.50 tons/ha. The highest Brix level was 14.13% in Bach Ngoc Duong melon variety fertilized with 139 K2O fertilizer formula (kg/ha).
The Role of The Head of Madrasah in Increasing The Quality of Education at MIN 3 Jembrana Bali
Authors: Supriyadi Supriyadi 
Published: October 2024

The head of the Madrasah as the top management has the authority and responsibility to the school he leads. To improve the quality and quality of the Madrasah in achieving maximum results, the head of the Madrasah must be competent, especially in related fields. The urgency of this study is to improve the quality of education, researchers use a qualitative descriptive approach. Informants taken in this study are the head of the madrasah, teachers, and students. Data collection techniques using observation, interviews, and documentation. While data analysis is done systematically starting from data reduction, data presentation, and verification or closing. The final results obtained from this study include: First, the role of the head of the Madrasah in MIN 3 Jembrana includes: educators, managers, administrators, mentors, leaders, innovators, and motivators commonly abbreviated as EMASLIM. Second, the quality of education in MIN 3 Jembrana is very potential, proven MIN 3 Jembrana is very potential evidenced by the number of academic and non-academic achievements both at the provincial and national level so that it can get the title of pilot project Min achievements in Jembrana Regency, and Third, The Role of the head of Madrasah in improving the quality of, harmonious and balanced.
Исследование пределов огнестойкости противопожарных клапанов системы вентиляции
Authors: Райзер Ю.С. , Гатауллина А.Р. , Абдуллин З.З. 
Published: October 2024

Рассмотрены результаты испытаний противопожарных клапанов торговой марки «Vertro»: KZО-2-60-800×800-O-S220-T-KR, типа KZО-2-60-O, KZО-2-90-800×800-O-S220-T-МC-KR, типа KZО-2-90-O и KZО-2-120-800×800-O-М220-T-KR, типа KZО-2-120-O на соответствие заявленному пределу огнестойкости. Противопожарные (огнезадерживающие) клапаны предназначены для перекрытия вентиляционных каналов с целью прекращения дальнейшего распространения огня, притока свежего воздуха к очагам возгорания, а также удаления дыма и других продуктов горения. Все три клапана нормально открытые, однокассетные с одной заслонкой, канальные, с приводом, расположенным снаружи. Корпус клапана изготавливается из оцинкованной стали. По периметру заслонки расположен термоактивный уплотнитель, который расширяется под действием высоких температур, обеспечивая герметичность клапана. Заслонка выполнена из огнестойкого стекломагнезита. В зависимости от предела огнестойкости применяется заслонка разной толщины. По результатам испытаний подтверждено, что клапаны соответствуют заявленному производителем пределу огнестойкости.
The results of testing fire dampers of the Vertro trademark: KZО-2-60-800×800-O-S220-T-KR, type KZО-2-60-O, KZО-2-90-800×800-O-S220-T-МC-KR, type KZО-2-90-O and KZО-2-120-800×800-O-М220-T-KR, type KZО-2-120-O against the declared fire resistance rating are considered. Fire dampers (fire-stopping) are designed to block the ventilation ducts in order to stop further fire spreading, supply fresh air to the fire sources, and provide for the smoke and other combustion products exhaust. The three valves are normally open, single-cassette with one flap, channel, with an outside drive. The valve body is made of galvanized steel. Along the flap perimeter there is a thermoactive seal that expands under the action of high temperatures and ensures the valve tightness. The flap is made of fire-resistant glass-magnesite. Depending on the fire resistance rating, a flap of different thickness is used. According to the test results, it was confirmed that the valves meet the fire resistance rating declared by the manufacturer.
Sifat pro-oksidan perasan jeruk lemon (Citrus Limun L.) untuk meningkatkan aktivitas antioksidan teh hitam
Authors: Anggraeni Frida Dwi , Suprihana  
Published: October 2024

Black tea was a drink made through fermentation by the enzyme polyphenol oxidase which could enzymatically oxidize catechins in fresh leaves. Lemon juice was added to the tea to determine the effect of adding lemon juice to the tea. This research aimed to determine the effect of brewing temperature and the pro-oxidant properties of adding lemon juice on changes in polyphenols and antioxidant activity of black tea. This research used a factorial design with 2 treatment factors, namely black tea brewed at 80oC and 100oC with the addition of 0%, 1% and 2% lemon juice with 3 repetitions. Green tea was used as a control. Analysis was carried out on total phenol content and IC50. Data analysis used the ANOVA test with a confidence level of 0.05. The research results showed that the total phenol of steeping black tea at a temperature of 80oC were lower than those steeping black tea at a temperature of 100oC, namely 20.41% and 27.08% respectively, but lower than the total phenol of green tea. Likewise, the higher level of added lemon juice, the higher total phenol content, but lower than total phenol content of green tea. For antioxidant activity was measured by IC50 inhibition, it showed that IC50 value at a black tea steeping temperature of 100oC was lower than IC50 value at 80oC black tea steeping. Likewise, with the addition of lemon juice, IC50 value decreased, namely 65.75 µg/ml to 35.84 µg/ml for steeping black tea at 80oC and 22.88 µg/ml to 15.94 µg/ml for steeping black tea at 100oC. The smaller IC50 value, the higher antioxidant activity.
Coherence of Multidimensional Pair Production Discharges in Polar Caps of Pulsars
Authors: Chernoglazov Alexander , Philippov Alexander , Timokhin Andrey 
Published: October 2024

Abstract
We report on the first self-consistent multidimensional particle-in-cell numerical simulations of nonhomogeneous pair discharges in polar caps of rotation-powered pulsars. By introducing strong inhomogeneities in the initial plasma distribution in our simulations, we analyze the degree of self-consistently emerging coherence of discharges across magnetic field lines. In 2D, we study discharge evolution for a wide range of physical parameters and boundary conditions corresponding to both the absent and free escape of charged particles from the surface of a neutron star. We also present the results of the first 3D simulations of discharges in a polar cap with a distribution of the global magnetospheric current appropriate for a pulsar with 60° inclination angle. For all parameters, we find the coherence scale of pair discharges across magnetic field lines to be of the order of the gap height. We also demonstrate that the popular “spark” model of pair discharges is incompatible with the universally adopted force-free magnetosphere model: intermittent discharges fill the entire zone of the polar cap that allows pair cascades, leaving no space for discharge-free regions. Our findings disprove the key assumption of the spark model about the existence of isolated distinct discharge columns.
The Production Population of Impact Craters in the Chang’E-6 Landing Mare
Authors: Luo Fanglu , Xiao Zhiyong , Wang Yichen , Ma Yizhen , Xu Rui , Wang Senmiao , Xie Minggang , Wu Yunhua , Deng Qingyun , Ma Pei 
Published: October 2024

Abstract
The Chang’E-6 mission accomplished the first sample return from the lunar farside. Earlier crater population measurements estimated the model age of the landing mare to range from the Eratosthenian to Late Imbrian, both of which are underrepresented by earlier returned samples. Establishing a new calibration point for lunar impact flux based on isotopic ages of the samples is promising, but the representative crater density for the landing mare (i.e., spatial density of craters with
D ≥ 1 km;
N
(1)) is equally important for this purpose, which lacks good constraints. After excluding the effects of background secondaries, crater equilibrium, and observational uncertainties on crater statistics, this work extracts production populations in different diameter ranges (∼200 m–2 km) from multiple subareas of the landing mare. Cross-validation of the production populations verifies that
N
(1) derived from direct measurements of craters with
D ≥ 1 km in sketched areas are reliable, which is (2.01 ± 0.90) × 10−3 and (6.05 ± 2.71) × 10−3 km2 for the western and eastern mare, respectively.
Candidate Distant Trans-Neptunian Objects Detected by the New Horizons Subaru TNO Survey
Authors: Fraser Wesley C. , Porter Simon B. , Peltier Lowell , Kavelaars JJ , Verbiscer Anne J. , Buie Marc W. , Stern S. Alan , Spencer John R. , Benecchi Susan D. , Terai Tsuyoshi , Ito Takashi , Yoshida Fumi , Gerdes David W. , Napier Kevin J. , Lin Hsing Wen , Gwyn Stephen D. J. , Smotherman Hayden , Fabbro Sebastien , Singer Kelsi N. , Alexander Amanda M. , Arimatsu Ko , Banks Maria E. , Bray Veronica J. , Ramy El-Maarry Mohamed , Ferrell Chelsea L. , Fuse Tetsuharu , Glass Florian , Holt Timothy R. , Hong Peng , Ishimaru Ryo , Johnson Perianne E. , Lauer Tod R. , Leiva Rodrigo , S. Lykawka Patryk , Marschall Raphael , Núñez Jorge I. , Postman Marc , Quirico Eric , Rhoden Alyssa R. , Simpson Anna M. , Schenk Paul , Skrutskie Michael F. , Steffl Andrew J. , Throop Henry 
Published: October 2024

Abstract
We report the detection of 239 trans-Neptunian objects discovered through the ongoing New Horizons survey for distant minor bodies being performed with the Hyper Suprime-Cam mosaic imager on the Subaru Telescope. These objects were discovered in images acquired with either the r2 or the recently commissioned EB-gri filter using shift and stack routines. Due to the extremely high stellar density of the search region downstream of the spacecraft, new machine learning techniques had to be developed to manage the extremely high false-positive rate of bogus candidates produced from the shift and stack routines. We report discoveries as faint as r2 ∼ 26.5. We highlight an overabundance of objects found at heliocentric distances R ≳ 70 au compared to expectations from modeling of the known outer solar system. If confirmed, these objects betray the presence of a heretofore-unrecognized abundance of distant objects that can help explain a number of other observations that otherwise remain at odds with the known Kuiper Belt, including detections of serendipitous stellar occultations, and recent results from the Student Dust Counter on board the New Horizons spacecraft.
Le Piyyout « Ehad Mi Yodea » en (judéo-)arabe
Authors: Sibony Jonas 
Published: October 2024

« Ehad Mi Yodea » (אֶחָד מִי יוֹדֵעַ) est un chant qui termine de la Haggada, le texte rituel du Seder de Pessah. Il s’agit d’un chant cumulatif composé de treize strophes, chacune liant les chiffres de un à treize à un concept ou à un principe du judaïsme. Le chant a été traduit de l’hébreu vers les langues vernaculaires des différentes communautés juives. L’objectif de cet article est de retracer le parcours de ce texte et d’en présenter différentes traductions judéo-arabes.