bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a novel variant lacking group A soyasaponin in a Chinese wild soybean (Glycine soja Sieb. & Zucc.): implications for breeding significance
Authors: Takahashi Yuya , Li Xiang‐Hua , Tsukamoto Chigen , Wang Ke‐Jing 
Published: August 2016

AbstractGroup A saponins are the principal factors underlying the undesirable bitter and astringent tastes in soybean food products. Therefore, the genetic reduction or elimination of the undesirable tastes is of great significance in soybean taste breeding. Using thin‐layer chromatography and liquid chromatography–mass spectrometry, we identified a novel mutant lacking group A saponins in a wild soybean (Glycine soja Sieb. & Zucc.), reflecting the failed arabinose binding of arabinosyltransferase to the C‐22 hydroxyl group, consequently leading to a lack of group A saponins and the concomitance expression of two new components A‐αg and KA‐αg in seeds. The C‐3 glycosylated soyasapogenol A (SS‐A), A‐αg, is a steady precursor compound in biosynthesis of Aa and Ab types in the seeds of soybean plants and exhibits stable inheritance. In this study, we observed a critical step necessary for arabinose binding to the C‐22 hydroxyl group in the biosynthetic pathway of group A saponins and identified an important germplasm accession for the genetic improvement in the tastes of soybean milk and processed soybean foods.
Bromodomain and extra‐terminal protein mimic JQ1 decreases inflammation in human vascular endothelial cells: Implications for pulmonary arterial hypertension
Authors: Mumby Sharon , Gambaryan Natalia , Meng Chao , Perros Frederic , Humbert Marc , Wort S. John , Adcock Ian M. 
Published: August 2016

ABSTRACT
Background and objectiveNuclear factor kappa B (NF‐kB)‐mediated inflammatory gene expression and vascular endothelial cell proliferation/remodelling are implicated in the pathophysiology of the fatal disease, pulmonary arterial hypertension (PAH). Bromodomain and extra‐terminal (BET) proteins are essential for the expression of a subset of NF‐kB‐induced inflammatory genes. BET mimics including JQ1+ prevent binding of BETs to acetylated histones and down‐regulate the expression of selected genes.MethodsThe effects of JQ1+ on the proliferation of primary human pulmonary microvascular endothelial cells (HPMECs) from healthy subjects were measured by bromodeoxyuridine (BrdU) incorporation. Cell cycle progression was assessed by flow cytometry; mRNA and protein levels of cyclin‐dependent kinases (CDKs), inhibitors and cytokines were determined by reverse transcription‐quantitative PCR (RT‐qPCR), Western blotting or ELISA. Histone acetyltransferase (HAT) and deacetylase (HDAC) activities were determined in nuclear extracts from whole lung of PAH and control patients.Results
JQ1+ significantly inhibited IL6 and IL8 (IL6 and CXCL8) mRNA and protein in HPMECs compared with its inactive enantiomer JQ1−. JQ1+ decreased NF‐kB p65 recruitment to native
IL6
and
IL8
promoters. JQ1+ showed a concentration‐dependent decrease in HPMEC proliferation compared with JQ1−‐treated cells. JQ1+ induced G1 cell cycle arrest by increasing the expression of the CDK inhibitors (CDKN) 1A (p21cip) and CDKN2D (p19INK4D
) and decreasing that of CDK2, CDK4 and CDK6. JQ1+ also inhibited serum‐stimulated migration of HPMECs. Finally, HAT activity was significantly increased in the lung of PAH patients.ConclusionInhibition of BETs in primary HPMECs decreases inflammation and remodelling. BET proteins could be a target for future therapies for PAH.
Too little sleep and an unhealthy diet could increase the risk of sustaining a new injury in adolescent elite athletes
Authors: von Rosen P. , Frohm A. , Kottorp A. , Fridén C. , Heijne A. 
Published: August 2016

Little is known about health variables and if these variables could increase the risk of injuries among adolescent elite athletes. The primary aim was to present overall data on self‐perceived stress, nutrition intake, self‐esteem, and sleep, as well as gender and age differences, on two occasions among adolescent elite athletes. A secondary aim was to study these health variables as potential risk factors on injury incidence. A questionnaire was e‐mailed to 340 adolescent elite athletes on two occasions during a single school year: autumn semester and spring semester. The results show that during autumn semester, the recommended intake of fruits, vegetables, and fish was not met for 20%, 39%, and 43% of the adolescent elite athletes, respectively. The recommended amount of sleep during weekdays was not obtained by 19%. Multiple logistic regression showed that athletes sleeping more than 8 h of sleep during weekdays reduced the odds of injury with 61% (OR, 0.39; 95% CI, 0.16–0.99) and athletes reaching the recommended nutrition intake reduced the odds with 64% (OR, 0.36; 95% CI, 0.14–0.91). Our findings suggest that nutrition intake and sleep volume are of importance in understanding injury incidence.
Economic evaluation of exercise‐based cardiac rehabilitation in patients with a recent acute coronary syndrome
Authors: Hautala A. J. , Kiviniemi A. M. , Mäkikallio T. , Koistinen P. , Ryynänen O.‐P. , Martikainen J. A. , Seppänen T. , Huikuri H. V. , Tulppo M. P. 
Published: August 2016

Health care decision‐making requires evidence of the cost‐effectiveness of medical therapies. We evaluated the cost‐effectiveness of exercise‐based cardiac rehabilitation (ECR) implemented according to guidelines. All the patients (n = 204) had experienced a recent acute coronary syndrome and were randomized to a 1‐year ECR (n = 109) or usual care (UC) group (n = 95). The patients’ health‐related quality of life was followed using the 15D instrument and health care costs were collected from electronic health registries. The cost‐effectiveness of ECR was estimated based on intervention and health care costs and quality‐adjusted life years (QALYs) gained. The total average cost per patient was lower in ECR than in UC. The incremental cost was divided by the baseline‐adjusted incremental QALYs (0.045), yielding an incremental cost‐effectiveness ratio of −€24511/QALYs. A combined endpoint of mortality, recurrent coronary event, or hospitalization for a heart failure occurred for five patients in ECR and 16 patients in UC (HR 3.9, 95% CI 1.4–10.6, P = 0.004, relative risk reduction 73%, number needed to treat eight). ECR is a dominant treatment option and decreases the occurrence of adverse cardiac events. These results are useful for decision‐making when planning optimal utilization of resources in Finnish health care.
The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability
Authors: Shen Yun , Ruan Qingxia , Chai Haoxi , Yuan Yongze , Yang Wannian , Chen Junping , Xin Zhanguo , Shi Huazhong 
Published: August 2016

SummaryPolyamines involve in gene regulation by interacting with and modulating the functions of various anionic macromolecules such as DNA, RNA and proteins. In this study, we identified an important function of the polyamine transporter LHR1 (LOWER EXPRESSION OF HEAT RESPONSIVE GENE1) in heat‐inducible gene expression in Arabidopsis thaliana. The lhr1 mutant was isolated through a forward genetic screening for altered expression of the luciferase reporter gene driven by the promoter from the heat‐inducible gene AtHSP18.2. The lhr1 mutant showed reduced induction of the luciferase gene in response to heat stress and was more sensitive to high temperature than the wild type. Map‐based cloning identified that the LHR1 gene encodes the polyamine transporter PUT3 (POLYAMINE UPTAKE TRANSPORTER 3) localized in the plasma membrane. The LHR1/PUT3 is required for the uptake of extracellular polyamines and plays an important role in stabilizing the mRNAs of several crucial heat stress responsive genes under high temperature. Genome‐wide gene expression analysis using RNA‐seq identified an array of differentially expressed genes, among which the transcript levels of some of the heat shock protein genes significantly reduced in response to prolonged heat stress in the lhr1 mutant. Our findings revealed an important heat stress response and tolerance mechanism involving polyamine influx which modulates mRNA stability of heat‐inducible genes under heat stress conditions.
Financial Development, Financial Structure and Income Inequality in China
Authors: Liu Guanchun , Liu Yuanyuan , Zhang Chengsi 
Published: August 2016

AbstractUsing panel data of 23 provinces during the period 1996–2012, this paper investigates the impacts of financial development and structure on income inequality in different administrative districts (urban, rural and overall) in China. The results produced by the dynamic generalised method of moments estimator provide some evidence for a linear and inverse ‘U‐shape’ relationship between financial development and income inequality, and increasing the relative importance of financial markets to banks helps to reduce income inequality. Furthermore, the results of panel threshold regressions show that the benefits of financial development only occur if the stock market activity has reached a threshold level and disappear if the stock market capitalisation has reached a certain scale. Meanwhile, the impact of the financial structure weakens as the financial development expands and the proportion of financial markets increases. We also find that the impacts of financial development and structure on rural income inequality are stronger than those on urban income inequality.
How do people make a decision on bottled or tap water? Preference elicitation with nonparametric bootstrap simulations
Authors: Güngör‐Demirci Gamze , Lee Juneseok , Mirzaei Majid , Younos Tamim 
Published: August 2016

AbstractPeople in the United States drink almost four times the amount of bottled water than they did 20 years ago, even though tap water supplies in the United States are considered to be among the safest in the world. To understand ‘how do people make a decision on tap or bottled water’, a consumer preferences survey was administered to the Civil and Environmental Engineering students attending a US university. The survey elicited information on participants’ preferences and real life attitude/preferences and included a multi‐criteria pairwise comparison. The pairwise comparison preferences were further analysed by integrating nonparametric bootstrap simulations to determine the underlying uncertainty. The results revealed that although safety issues were deemed most important, participants were also subconsciously aware of other crucial issues related to drinking water. These findings provide useful information for drinking water policy experts and water utilities about consumer perceptions of the relative virtues of tap and bottled water.
Selenium bioaccumulation in eutrophic lake – Dąbie Małe Lake, Poland
Authors: Tomza‐Marciniak Agnieszka , Marciniak Andrzej M. , Pilarczyk Bogumiła , Molenda Marika 
Published: August 2016

AbstractWhile the world literature presents many studies on the occurrence of selenium in aquatic ecosystems, the risks associated with overexposure of hydrobionts to this element and the consequences of its magnification in the food chain (areas rich in Se), there are few studies on Se concentration in Poland's water bodies and watercourses (Se deficient area). The objective of the study was to assess the Se bioaccumulation in Dąbie Małe Lake, based on Se concentration in the water, bottom sediments, and organs of Rutilus rutilus and Perca fluviatilis. The accumulation and biomagnification factor averaged 231 ± 69 and 1.2, respectively. Bioconcentration factor, calculated as Se in muscle divided by Se in water, ranged 35–104. Although Se concentrations in bottom sediments and fish were generally lower than the values reported in the literature, Se concentration in the water exceeded 2 µg/L, recommended value as a probable safe water concentration for wildlife.
Increased activity of TRPV3 in keratinocytes in hypertrophic burn scars with postburn pruritus
Authors: Kim Hye One , Cho Yong Se , Park Sook Young , Kwak In Suk , Choi Min Gyu , Chung Bo Young , Park Chun Wook , Lee Jun Young 
Published: August 2016

AbstractPostburn pruritus is a common distressing sequela of burn wounds. Empirical antipruritic treatment often fails to have a satisfactory outcome, as the mechanism of it has not been fully elucidated. The aim of this study was to evaluate the manifestation of transient receptor potential vanilloid 3 (TRPV3), transient receptor potential ankyrin 1 (TRPA1), and other related receptors in postburn pruritus. Sixty‐five burn patients with (n = 40) or without (n = 25) pruritus were investigated, including skin biopsies. Keratinocytes and fibroblasts from skin biopsy samples were separated. Real time‐PCR showed that mRNA of TRPV3 was significantly increased in keratinocytes from pruritic burn scars than in keratinocytes from nonpruritic burn scars. With TRPV3 activation, intracellular Ca2+ concentrations were more significantly increased in keratinocytes from pruritic burn scars than in those from nonpruritic ones. Additionally, mRNA and protein levels of protease‐activated receptor 2 (PAR2) and neurokinin‐1 receptor (NK1R) were also significantly increased in pruritic burn scars. In conclusion, it was confirmed that TRPV3, PAR2, and NK1R were highly expressed in pruritic burn scars. These results may help determine a novel mechanism for postburn pruritus.
The Effect of Vestibular Stimulation on Motor Functions of Children With Cerebral Palsy
Authors: Tramontano Marco , Medici Alessandra , Iosa Marco , Chiariotti Alessia , Fusillo Giulia , Manzari Leonardo , Morelli Daniela 
Published: August 2016

Background:Cerebral palsy (CP) has been defined as a nonprogressive disease of movement and posture development. Physical therapy techniques use different forms of sensory stimulation to improve neuromotor development.Aim:The aim of this study was to assess the efficacy of a vestibular stimulation training in improving motor functions in cerebral palsy.Population:Fourteen children with CP were randomly separated into two different groups in a cross-over trial.Methods:Over a period of 10 weeks, each group performed 10 sessions of 50 min of neurodevelopmental treatment (NDT) and 10 sessions of vestibular training (VR). Children were evaluated with the Gross Motor Function Measurement-88 scale, the Goal Attainment Scale and the root mean square of head accelerations.Results:A significant improvement in the GAS-score (p = .003) was noted after NDT+VR.Conclusions:Vestibular stimulation integrated with NDT proved to be an effective complementary strategy for facilitating motor functioning.
The 1,500-m Rowing Performance is Highly Dependent on Modified Wingate Anaerobic Test Performance in National-Level Adolescent Rowers
Authors: Maciejewski Hugo , Rahmani Abderrahmane , Chorin Frédéric , Lardy Julien , Giroux Caroline , Ratel Sébastien 
Published: August 2016

Purpose:The aim of the current study was to determine whether the anaerobic performance assessed by a modified Wingate test could account for the 1,500-m rowing ergometer performance in young competitive rowers.Methods:Fourteen national-level adolescent rowers performed on a rowing ergometer i) a 30-s allout exercise (i.e., modified Wingate test) and ii) a 1,500-m all-out exercise (i.e., rowing performance). For each of these two exercises, the mean power output was considered as the main performance criterion (PWIN and P1500, respectively).Results:PWIN was correlated to P1500 (r2 = .83, p < .0001, β = 100%). Furthermore, the estimated total muscle mass was correlated to PWIN and P1500 (r2 = .72 and r2 = .83, p < .0001, β = 100%, respectively). The allometric scaling also indicated that total muscle mass accounts 62% for the relationship between PWIN and P1500.Conclusion:Our results highlight the importance of considering the contribution of total muscle mass and anaerobic energy pathways to 1,500-m rowing performance in competitive adolescent rowers. Therefore, the modified Wingate test could be used by rowing coaches to potentially identify talented young rowers.
Complete Genome Sequence of a
Bohle iridovirus
Isolate from Ornate Burrowing Frogs (
Limnodynastes ornatus
) in Australia
Authors: Hick Paul M. , Subramaniam Kuttichantran , Thompson Patrick , Whittington Richard J. , Waltzek Thomas B. 
Published: August 2016

ABSTRACT

Bohle iridovirus
(BIV) is a species within the genus
Ranavirus
, family
Iridoviridae
, first isolated from the ornate burrowing frog
Limnodynastes ornatus
in Australia. The BIV genome confirms it is closely related to isolates from boreal toad
Anaxyrus boreas
and leaf-tailed gecko
Uroplatus fimbriatus
within the United States and Germany, respectively.
First New World Primate Papillomavirus Identification in the Atlantic Forest, Brazil:
Alouatta guariba papillomavirus 1
Authors: Silvestre Rodrigo Vellasco Duarte , de Souza Alex Junior Souza , Júnior Edivaldo Costa Sousa , Silva Allan Kaio , de Mello Wyller Alencar , Nunes Marcio Roberto T. , Júnior João Lídio S. G. V. , Cardoso Jedson Ferreira , de Vasconcelos Janaina Mota , de Oliveira Layanna Freitas , da Silva Sandro Patroca , da Silva Adriana Marques J. , Fries Brigida Gomes , Summa Maria Eugênia L. , de Sá Lilian Rose M. 
Published: August 2016

ABSTRACT

We report here the complete genome sequence of the first papillomavirus detected in a New World primate, howler monkey,
Alouatta guariba clamitans papillomavirus 1
(AgPV1), from the Atlantic Forest in São Paulo State, Brazil.
Complete Genome Sequence of Influenza Virus H9N2 Associated with a Fatal Outbreak among Chickens in Dubai
Authors: Lau Siu-Ying , Joseph Sunitha , Chan Kwok-Hung , Chen Honglin , Patteril Nissy Annie Gerogy , Elizabeth Shyna K. , Muhammed Rubeena , Baskar Vijay , Lau Susanna K. P. , Kinne Joerg , Wernery Ulrich , Woo Patrick C. Y. 
Published: August 2016

ABSTRACT
We report the complete genome sequence of influenza virus H9N2 associated with a fatal outbreak among chickens in Dubai. All segments are clustered with avian H9N2 viruses circulating in the Middle East but distinct from those in southeast Asia. It is not a reassortant virus or transmitted from other regions.
Draft Genome Sequence of a Multidrug-Resistant
Klebsiella pneumoniae
Carbapenemase-Producing
Acinetobacter baumannii
Sequence Type 2 Isolate from Puerto Rico
Authors: Martínez Teresa , Ropelewski Alexander J. , González-Mendez Ricardo , Vázquez Guillermo J. , Robledo Iraida E. 
Published: August 2016

ABSTRACT

We report here the draft genome sequence of
Acinetobacter baumannii
strain M3AC14-8, sequence type 2 (ST2), carrying a chromosomally carried
bla
KPC-2
gene. The draft genome consists of a total length of 4.11 Mbp and a G+C content of 39.25%.
Draft Genome Sequences of Nine Clinical Isolates of Vancomycin-Resistant Enterococci
Authors: Higgins Paul G. , Koehler Daniela , Chan Jacqueline Z. M. , Cornely Oliver A. , Fätkenheuer Gerd , Gillis Meyke , Pallen Mark J. , Tien Johanna , Seifert Harald , Vehreschild Maria J. G. T. , Millard Andrew D. 
Published: August 2016

ABSTRACT
In 2012, there was an increase in vancomycin-resistant enterococci (VRE) isolated from the intensive care unit at the University Hospital of Cologne. Using whole-genome sequencing it was possible to establish that bloodstream infections with VRE were not the result of an outbreak or cross infections.
Complete Genome Sequence of Genotype VI Newcastle Disease Viruses Isolated from Pigeons in Pakistan
Authors: Wajid Abdul , Rehmani Shafqat Fatima , Sharma Poonam , Goraichuk Iryna V. , Dimitrov Kiril M. , Afonso Claudio L. 
Published: August 2016

ABSTRACT
Two complete genome sequences of Newcastle disease virus (NDV) are described here. Virulent isolates pigeon/Pakistan/Lahore/21A/2015 and pigeon/Pakistan/Lahore/25A/2015 were obtained from racing pigeons sampled in the Pakistani province of Punjab during 2015. Phylogenetic analysis of the fusion protein genes and complete genomes classified the isolates as members of NDV class II, genotype VI.
Understanding the relative valuation of research impact: a best–worst scaling experiment of the general public and biomedical and health researchers
Authors: Pollitt Alexandra , Potoglou Dimitris , Patil Sunil , Burge Peter , Guthrie Susan , King Suzanne , Wooding Steven , Grant Jonathan 
Published: August 2016

Objectives(1) To test the use of best–worst scaling (BWS) experiments in valuing different types of biomedical and health research impact, and (2) to explore how different types of research impact are valued by different stakeholder groups.DesignSurvey-based BWS experiment and discrete choice modelling.SettingThe UK.ParticipantsCurrent and recent UK Medical Research Council grant holders and a representative sample of the general public recruited from an online panel.ResultsIn relation to the study's 2 objectives: (1) we demonstrate the application of BWS methodology in the quantitative assessment and valuation of research impact. (2) The general public and researchers provided similar valuations for research impacts such as improved life expectancy, job creation and reduced health costs, but there was less agreement between the groups on other impacts, including commercial capacity development, training and dissemination.ConclusionsThis is the second time that a discrete choice experiment has been used to assess how the general public and researchers value different types of research impact, and the first time that BWS has been used to elicit these choices. While the 2 groups value different research impacts in different ways, we note that where they agree, this is generally about matters that are seemingly more important and associated with wider social benefit, rather than impacts occurring within the research system. These findings are a first step in exploring how the beneficiaries and producers of research value different kinds of impact, an important consideration given the growing emphasis on funding and assessing research on the basis of (potential) impact. Future research should refine and replicate both the current study and that of Milleret alin other countries and disciplines.