bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential proteome profiling in the hippocampus of amnesic mice
Authors: Baghel Meghraj Singh , Thakur Mahendra Kumar 
Published: April 2017

AbstractAmnesia or memory loss is associated with brain aging and several neurodegenerative pathologies including Alzheimer's disease (AD). This can be induced by a cholinergic antagonist scopolamine but the underlying molecular mechanism is poorly understood. This study of proteome profiling in the hippocampus could provide conceptual insights into the molecular mechanisms involved in amnesia. To reveal this, mice were administered scopolamine to induce amnesia and memory impairment was validated by novel object recognition test. Using two‐dimensional gel electrophoresis coupled with MALDI‐MS/MS, we have analyzed the hippocampal proteome and identified 18 proteins which were differentially expressed. Out of these proteins, 11 were downregulated and 7 were upregulated in scopolamine‐treated mice as compared to control. In silico analysis showed that the majority of identified proteins are involved in metabolism, catalytic activity, and cytoskeleton architectural functions. STRING interaction network analysis revealed that majority of identified proteins exhibit common association with Actg1 cytoskeleton and Vdac1 energy transporter protein. Furthermore, interaction map analysis showed that Fascin1 and Coronin 1b individually interact with Actg1 and regulate the actin filament dynamics. Vdac1 was significantly downregulated in amnesic mice and showed interaction with other proteins in interaction network. Therefore, we silenced Vdac1 in the hippocampus of normal young mice and found similar impairment in recognition memory of Vdac1 silenced and scopolamine‐treated mice. Thus, these findings suggest that Vdac1‐mediated disruption of energy metabolism and cytoskeleton architecture might be involved in scopolamine‐induced amnesia.
C‐reactive protein variability is associated with vascular access outcome in hemodialysis patients
Authors: Kuo Wei‐Hung , Lee Yueh‐Ting , Ng Hwee‐Yeong , Wang Chun‐Yeh , Wu Chien‐Hsing , Lee Chien‐Te 
Published: April 2017

BackgroundHemodialysis (HD) vascular access failure is one of the most important causes of morbidity and contributes to the cost of dialysis care. There is paucity of data evaluating long‐term monitoring of C‐reactive protein (CRP) on outcome of HD vascular access.MethodsWe conducted a retrospective study to investigate whether variability of serum CRP level was associated with vascular access failure rate over a 7‐year period. A total of 318 HD patients were included. Their demographic data, co‐morbidities and biochemical data were reviewed and collected. Serum high‐sensitivity CRP (hs‐CRP) level was measured every 6 months. Patients were divided into three groups according to their serial hs‐CRP levels. Patients with their hs‐CRP below 2 mg/L were defined as low group (n=65, 20.4%) and those with higher than 4 mg/L were defined as high (n=39, 12.3%). The rest were classified as fluctuated hs‐CRP group (n=214, 67.3%). Treatment of vascular access failure includes angioplasty and access re‐creation.ResultsTheir body mass index, indicators of dialysis adequacy and serum albumin and hs‐CRP levels differed significantly among three groups. The annual vascular access failure rate was significantly higher in fluctuated hs‐CRP group than in high hs‐CRP group (0.41 vs 0.36, P=.037). Serum albumin was a significant associate of vascular access failure. Kaplan‐Meier survival analysis indicated patients with high or fluctuated hs‐CRP had shorter free interval of vascular access failure than low hs‐CRP group.ConclusionsHD patients with fluctuated hs‐CRP levels were associated with increased vascular access failure.
Maintaining postharvest quality of cold stored ‘Hass’ avocados by altering the fatty acids content and composition with the use of natural volatile compounds – methyl jasmonate and methyl salicylate
Authors: Glowacz Marcin , Bill Malick , Tinyane Peter P , Sivakumar Dharini 
Published: April 2017

AbstractBACKGROUNDLow temperatures are often used to reduce metabolic processes and extend the storage life of fruit; however, in the case of avocado, a temperature below 3 °C will often result in the development of physiological disorders associated with chilling injury. The objective of this study was to investigate the ability of methyl jasmonate (MeJA) and methyl salicylate (MeSA) vapours to alleviate chilling injury in ‘Hass’ avocado fruit kept at 2 °C for 21 days followed by 6–7 days of shelf‐life at 20 °C, simulating supply chain conditions.RESULTSThe incidence and severity of chilling injury were significantly reduced in MeJA‐ and MeSA‐exposed fruit, especially at 100 µmol L−1. The mechanism involved improved membrane integrity via alteration of the fatty acid content and composition, down‐regulation of LOX gene expression and reduced activity of lipoxygenase.CONCLUSIONMeJA and MeSA have the potential for being used with ‘Hass’ avocado fruit shipped at low temperature to reduce its susceptibility to chilling injury. © 2017 Society of Chemical Industry
Predicting off‐site impacts on breeding success of the marsh harrier
Authors: Senzaki Masayuki , Yamaura Yuichi , Nakamura Futoshi 
Published: April 2017

ABSTRACTConstruction of buildings, and other types of land uses by humans can exert negative impacts on wildlife that live in the areas surrounding such developments (i.e., off‐site impacts). To reduce or lessen such impacts, it is important to determine the biological metrics (e.g., abundance, breeding success) that are affected and at which spatial scales. We monitored the eastern marsh harrier (Circus spilonotus) breeding in wetland patches in northern Japan for 4 years and quantified its abundance (no. pairs) and breeding success (no. juveniles) in natural environments compared to artificial land uses in the surrounding areas. We developed a hierarchical model to simultaneously infer the effects of foraging habitats and artificial land uses on the numbers of pairs and juveniles. We found that the amount of foraging habitat within 0.5 km of wetland patches positively influenced the number of pairs per patch and that the amount of artificial land use within 2.0 km from the patches negatively influenced the number of pairs per patch and the number of juveniles per pair. The number of juveniles per patch was therefore affected by these 2 features and was most susceptible to increased land use by humans within 2.0 km from the patches. Our results can be used to predict the magnitudes of off‐site impacts on eastern marsh harriers before any additional development occurs. To effectively manage off‐site impacts, we highlight the importance of considering species abundance and the biological processes mediating breeding success that are possibly affected by different land uses. © 2017 The Wildlife Society.
In vitro comparison of renal stone laser treatment using fragmentation and popcorn technique
Authors: Klaver Paul , de Boorder Tjeerd , Rem Alex I. , Lock Tycho M. T. W. , Noordmans Herke Jan 
Published: April 2017

ObjectiveTo study the effectiveness of two laser techniques clinically used to fragment renal stones: fragmenting technique (FT) and popcorn technique (PT).MethodsPhantom stones were placed in a test tube filled with water, mimicking a renal calyx model. A Holmium:YAG laser was used for fragmentation using both techniques. Four series of experiments were performed with two parameters: the technique (FT or PT) and the number of stones in the test tube (one or four). The mass decrease of the phantom stones was measured before, during, and after the experiment to quantify the effect of both techniques.ResultsVisualization of PT showed that the main effect of PT takes place, when the stone moves in front of the laser fiber and is subject to direct radiant exposure. Both FT and PT resulted in a decrease in stone weight; the mass decrease of the stones subjected to FT exceeded that of the stones subjected to PT, even with less laser energy applied. This difference in mass decrease was evident in both the experiments with one and four stones.ConclusionsPT was less effective in decreasing stone weight compared with FT. The FT is more effective regarding the applied energy than PT, even in a shorter time period and regardless of the number of stones. This study suggests that FT is to be preferred over PT, when stones are accessible by the laser fiber. Lasers Surg. Med. 49:698–704, 2017. © 2017 Wiley Periodicals, Inc.
Psychometric properties of the Mental Health and Social Inadaptation Assessment for Adolescents (MIA) in a population‐based sample
Authors: Côté Sylvana M. , Orri Massimiliano , Brendgen Mara , Vitaro Frank , Boivin Michel , Japel Christa , Séguin Jean R. , Geoffroy Marie‐Claude , Rouquette Alexandra , Falissard Bruno , Tremblay Richard E. 
Published: April 2017

AbstractWe report on the psychometric properties of the Mental Health and Social Inadaptation Assessment for Adolescents (MIA), a self‐report instrument for quantifying the frequency of mental health and psychosocial adaptation problems using a dimensional approach and based on the DSM‐5. The instrument includes 113 questions, takes 20–25 minutes to answer, and covers the past 12 months. A population‐based cohort of adolescents (n = 1443, age = 15 years; 48% males) rated the frequency at which they experienced symptoms of Attention Deficit Hyperactivity Disorder (ADHD), Conduct Disorder, Oppositional Defiant Disorder, Depression, Generalized Anxiety, Social Phobia, Eating Disorders (i.e. DSM disorders), Self‐harm, Delinquency, Psychopathy as well as social adaptation problems (e.g. aggression). They also rated interference with functioning in four contexts (family, friends, school, daily life). Reliability analyses indicated good to excellent internal consistency for most scales (alpha = 0.70–0.97) except Psychopathy (alpha = 0.46). The hypothesized structure of the instrument showed acceptable fit according to confirmatory factor analysis (CFA) [Chi‐square (4155) = 9776.2, p = 0.000; Chi‐square/DF = 2.35; root mean square error of approximation (RMSEA) = 0.031; Comparative Fit Index (CFI) = 0.864], and good convergent and discriminant validity according to multitrait‐multimethods analysis. This initial study showed adequate internal validity and reliability of the MIA. Our findings open the way for further studies investigating other validity aspects, which are necessary before recommending the wide use of the MIA in research and clinical settings.
Malignant ovarian germ cell tumors in pediatric patients: The AIEOP (Associazione Italiana Ematologia Oncologia Pediatrica) study
Authors: Terenziani M. , Bisogno G. , Boldrini R. , Cecchetto G. , Conte M. , Boschetti L. , De Pasquale M.D. , Biasoni D. , Inserra A. , Siracusa F. , Basso M.E. , De Leonardis F. , Di Pinto D. , Barretta F. , Spreafico F. , D'Angelo P. 
Published: April 2017

AbstractObjectiveMalignant ovarian germ cell tumors (MOGCT) carry an excellent prognosis, and the treatment aims to achieve results with the least possible treatment‐related morbidity. The aim of this study was to assess the outcomes of pediatric patients with MOGCT.MethodsPatients were treated according to their stage: surgery and surveillance for stage I; a modified bleomycin–etoposide–cisplatin (BEP) regimen for stages II (three cycles), III, and IV (three cycles) with surgery on residual disease.ResultsSeventy‐seven patients were enrolled (median age 11.8 years), 26 with dysgerminoma (Dysg), 13 with immature teratoma and elevated serum alpha‐fetoprotein levels (IT + AFP), and 38 with nondysgeminoma (Non‐Dysg) staged as follows: 27 stage I, 13 stage II, 32 stage III, 5 stage IV. Among evaluable patients in stage I (5‐year event‐free survival [EFS] 72.1% [95% CI: 56.4–92.1%]; 5‐year overall survival [OS] 100%), seven relapsed (three patients with Dysg and four patients with Non‐Dysg) and were rescued with chemotherapy (plus surgery in three patients). Among the evaluable patients with stages II–IV, 48 (98%) achieved complete remission after chemotherapy ± surgery, one (IT + AFP, stage IV) had progressive disease. In the whole series (median follow‐up 80 months), the 5‐year OS and EFS were 98.5% (95% CI: 95.6–100%) and 84.5% (95% CI: 76.5–93.5%).ConclusionsWe confirm the excellent outcome for MOGCT. Robust data are lacking on surgical staging, surveillance for Non‐Dysg with stage I, the management of IT + AFP, and the most appropriate BEP regimen. As pediatric oncologists, we support the role of surveillance after proper surgical staging providing cases are managed by experts at specialized pediatric centers.
Effects of malnutrition on treatment‐related morbidity and survival of children with cancer in Nicaragua
Authors: Pribnow Allison K. , Ortiz Roberta , Báez Luis Fulgencio , Mendieta Luvy , Luna‐Fineman Sandra 
Published: April 2017

AbstractBackgroundMost children with cancer live in resource‐limited countries where malnutrition is often prevalent. We identified the relationship between malnutrition and treatment‐related morbidity (TRM), abandonment of therapy, and survival of children with cancer in Nicaragua to better inform targeted nutritional interventions.ProcedureWe conducted a retrospective review of patients aged 6 months to 18 years with newly diagnosed acute lymphoblastic leukemia, acute myeloid leukemia (AML), Wilms tumor, Hodgkin lymphoma, or Burkitt lymphoma (BL) who were treated between January 1, 2004, and December 31, 2007 at Children's Hospital Manuel de Jesus Rivera in Managua, Nicaragua. Statistical analysis examined the relations among nutritional status and cancer type, risk category, TRM, and event‐free survival (EFS).ResultsSixty‐seven percent of patients (189/282) were malnourished at diagnosis. Malnutrition was highest among patients with Wilms tumor (85.7%), BL (75%), and AML (74.3%). A total of 92.2% of patients (225/244) experienced morbidity during the first 90 days. Malnutrition was associated with severe infection (P = 0.033). Severely malnourished patients had ≥grade 3 TRM on more days (P = 0.023) and were more likely to experience severe TRM on >50% of days (P = 0.032; OR, 3.27 [95% CI, 1.05–10.16]). Malnourished patients had inferior median EFS (2.25 vs. 5.58 years; P = 0.049), and abandoned therapy more frequently (P = 0.015).ConclusionsIn Nicaragua, pediatric oncology patients with malnutrition at diagnosis experienced increased TRM, abandoned therapy more frequently, and had inferior EFS. Standardized nutritional evaluation of patients with newly diagnosed cancer and targeted provision of nutritional support are essential to decrease TRM and improve outcomes.
Hepatic expression profiles in retroviral infection: relevance to drug hypersensitivity risk
Authors: Wong Yat Yee , Johnson Brian , Friedrich Thomas C. , Trepanier Lauren A. 
Published: April 2017

AbstractHIV‐infected patients show a markedly increased risk of delayed hypersensitivity (HS) reactions to potentiated sulfonamide antibiotics (trimethoprim/sulfamethoxazole or TMP/SMX). Some studies have suggested altered SMX biotransformation in HIV infection, but hepatic biotransformation pathways have not been evaluated directly. Systemic lupus erythematosus (SLE) is another chronic inflammatory disease with a higher incidence of sulfonamide HS, but it is unclear whether retroviral infection and SLE share risk factors for drug HS. We hypothesized that retroviral infection would lead to dysregulation of hepatic pathways of SMX biotransformation, as well as pathway alterations in common with SLE that could contribute to drug HS risk. We characterized hepatic expression profiles and enzymatic activities in an SIV‐infected macaque model of retroviral infection, and found no evidence for dysregulation of sulfonamide drug biotransformation pathways. Specifically, NAT1, NAT2, CYP2C8, CYP2C9, CYB5R3, MARC1/2, and glutathione‐related genes (GCLC, GCLM, GSS, GSTM1, and GSTP1) were not differentially expressed in drug naïve SIVmac239‐infected male macaques compared to age‐matched controls, and activities for SMX N‐acetylation and SMX hydroxylamine reduction were not different. However, multiple genes that are reportedly over‐expressed in SLE patients were also up‐regulated in retroviral infection, to include enhanced immunoproteasomal processing and presentation of antigens as well as up‐regulation of gene clusters that may be permissive to autoimmunity. These findings support the hypothesis that pathways downstream from drug biotransformation may be primarily important in drug HS risk in HIV infection.
Leader‐following consensus with connectivity preservation of uncertain Euler–Lagrange multi‐agent systems
Authors: Dong Yi , Huang Jie 
Published: April 2017

SummaryThe leader‐following consensus problem for multiple Euler–Lagrange systems has been extensively studied for various scenarios. Under the assumption that the communication graph is jointly connected, one of our recent papers gave the solution for the case where the leader system can generate a combination of arbitrary step signal, arbitrary ramp signal, and arbitrary sinusoidal signals. In practice, it is desirable to enable the control law the capability of maintaining the connectivity of the communication graph, thus achieving the leader‐following consensus without assuming the connectivity of the communication graph. We call such a problem as leader‐following consensus with connectivity preservation. By combining the adaptive control technique and potential function technique, we will show that such a problem is solvable. By employing different potential functions, our approach may also lead to the solution of such problems as rendezvous, flocking and swarming. Copyright © 2017 John Wiley & Sons, Ltd.
Stabilization by forwarding design for switched feedforward systems with unstable modes
Authors: Long Lijun 
Published: April 2017

SummaryThe problem of global stabilization is investigated for a class of switched nonlinear feedforward systems in this paper where the solvability of the stabilization problem for individual subsystem is not assumed. Some sufficient condition for the stabilization problem to be solvable is derived for the first time by exploiting the multiple Lyapunov functions method and the forwarding technique. Also, we design a switching law and construct bounded state feedback controllers of subsystems explicitly by a recursive design algorithm to achieve global asymptotic stability. The provided technique permits removal of a common restriction in which all subsystems in switched nonlinear feedforward systems are globally asymptotically stable. Finally, a numerical example is provided to demonstrate the feasibility of the theoretical result. Copyright © 2017 John Wiley & Sons, Ltd.
AuRnX and XAuRn (X=F ‐ I and OH)
Authors: Li Xinying 
Published: April 2017

AbstractSystematic theoretical investigations on the AuRnX and XAuRn (X=F‐I and OH) have been performed at B3LYP, MP2 and CCSD(T) theoretical levels. The linear structures, stretching frequencies and IR intensities were predicted. Analyses on dissociation energies and frontier orbitals show a decreased trend of stability down the periodic table for halogens. Natural bond orbital analyses show the σAu‐Rnand σAu‐Xbonding occured between Auspdhybrids and Rn/Xsphybrids. The Au−Rn,Au−X and Rn−X interactions, characterized by negative energy densities and positive Laplacian values together with relative small electron densities at BCPs, can be described as interactions of moderate strength with partial covalence. Analyses on electron localization functions, electron density deformations and bonding orbital coefficients found an increased covalence trend down the periodic table for halogens.
Postoperative Prolonged Inflammatory Response as a Poor Prognostic Factor After Curative Resection for Gastric Cancer
Authors: Okumura Yasuhiro , Hiki Naoki , Kumagai Koshi , Ida Satoshi , Nunobe Souya , Ohashi Manabu , Sano Takeshi 
Published: April 2017

AbstractBackgroundPostoperative inflammatory complications are associated with poorer prognosis in gastrointestinal malignancies. The aims of this study were to clarify the impact of postoperative inflammation itself on overall survival (OS) and relapse‐free survival (RFS) in advanced gastric cancer patients.MethodsWe retrospectively analyzed 490 patients who underwent curative resection for pStage II and III gastric cancer from 2005 to 2008. We evaluated postoperative inflammation based on duration of hyperthermia (body temperature ≥38 °C) and leukocytosis (≥12,000/µL−1). OS and RFS were compared between a prolonged inflammation group and non‐prolonged inflammation group. Multivariate analysis using the Cox proportional hazard model was performed to identify independent prognostic factors.ResultsThe prolonged inflammation group comprised 57 (11.7%) patients who had hyperthermia for 4 days or longer and 42 (8.6%) patients who had leukocytosis for 7 days or longer. OS and RFS were significantly worse in the prolonged hyperthermia group (OS: hazard ratio (HR) 1.84, 95% confidence interval (CI) 1.19–2.73, P = 0.004; RFS: HR 1.66, 95% CI 1.08–2.45, P = 0.015). The prolonged leukocytosis group also showed significantly worse OS (HR 1.92, 95% CI 1.19–2.96, P = 0.004) and RFS (HR 1.90, 95% CI 1.19–2.88, P = 0.004). Multivariate analysis identified prolonged hyperthermia as an independent factor for predicting poor prognosis (OS: HR 1.77, 95% CI 1.13–2.68, P = 0.013; RFS: HR 1.60; 95% CI 1.03–2.39, P = 0.038).ConclusionsProlonged hyperthermia and leukocytosis after curative gastrectomy were associated with poorer OS and RFS in advanced gastric cancer patients.
An Exploration of Genetic Test Utilization, Genetic Counseling, and Consanguinity within the Inborn Errors of Metabolism Collaborative (IBEMC)
Authors: Stein Quinn P. , Vockley Cate Walsh , Edick Mathew J. , Zhai Shaohui , Hiner Sally J. , Loman Rebecca S. , Davis‐Keppen Laura , Zuck Taylor A. , Cameron Cynthia A. , Berry Susan A. ,   for the Inborn Errors of Metabolism Collaborative
Published: April 2017

AbstractThe Inborn Errors of Metabolism Collaborative (IBEMC) includes clinicians from 29 institutions collecting data to enhance understanding of metabolic conditions diagnosable by newborn screening. Data collected includes hospitalizations, test results, services, and long‐term outcomes. Through evaluation of this data, we sought to determine how frequently genetic counseling had been provided, how often genetic testing was performed, and also determine the consanguinity rate in this population. A data query was performed with the following elements abstracted/analyzed: current age, metabolic condition, whether genetic counseling was provided (and by whom), whether genetic testing was performed, and consanguinity. Genetic counseling was provided to families 95.8% of the time and in 68.6% of cases by a genetic counselor. Genetic testing was performed on 68.0% of subjects, with usage highest for fatty‐acid‐oxidation disorders (85.1%). The rate of consanguinity was 2.38%. Within this large national collaborative there is a high frequency of genetic counseling, though in one‐third of cases a genetic counselor has not been involved. Additionally, while metabolic conditions have historically been diagnosed biochemically, there is currently high utilization of molecular testing suggesting DNA testing is being incorporated into diagnostic assessments ‐ especially for fatty‐acid‐oxidation disorders where the underlying genotype helps predict clinical presentation.