bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA profiling of uveal melanoma exosomes as a metastatic risk biomarker
Authors: Kilic E. , Smit K. , van Poppelen N. , Lunavat T. , Derks K. , Vaarwater J. , Verdijk R. , Mensink H. , Lötvall J. , de Klein A. 
Published: September 2017

PurposeUveal melanoma (UM) is the most common primary intraocular malignancy in adults. It is a highly aggressive cancer in which nearly 50% of patients die from liver metastasis. UM patients can be divided into 3 groups based on their genetic profile and disease free survival; the low risk, intermediate risk and high risk tumours. Recently, we have shown that high risk patients can be identified based on the expression of five miRNAs. Since tumour tissue is not always available and biopsies are not without risk, it is important to develop a method that can identify high risk patients in a non‐invasive manner. Exosomal miRNAs are an excellent candidate for this application.MethodsExosomes were isolated from the cell culture medium of a non‐metastatic and high risk metastatic UM cell line by ultracentrifugation and were characterized by western blot, electron microscopy and Nanosight Tracking Analysis (NTA). RNA was isolated from exosomes by the Qiagen RNeasy micro kit and quantified by an Agilent bioanalyzer. Subsequently, miRNA expression was measured by Taqman miRNA qPCR assays.ResultsAll exosome samples isolated from cell medium showed expression of CD81. Bioanalyzer confirmed the absence of ribosomal RNA and an abundance of small RNAs. qPCR analysis shows changes in the expression of some classifier miRNAs in exosomes extracted from cell lines. Showing that the classifier miRNA signature in UM cells partially overlaps with the miRNA signature in exosomes secreted by UM cells.ConclusionsThese exosomal miRNAs show great promise as a reliable predictor of disease free survival in UM. Next step is to detect these exosomal markers in blood of UM patients as this will enable us to provide all UM patients with a prognosis in a non‐invasive manner.
Semi‐efficient valuations and put‐call parity
Authors: Herdegen Martin , Schweizer Martin 
Published: September 2017

AbstractWe propose an approach to the valuation of payoffs in general semimartingale models of financial markets where prices are nonnegative. Each asset price can hit 0; we only exclude that this ever happens simultaneously for all assets. We start from two simple, economically motivated axioms, namely, absence of arbitrage (in the sense of NUPBR) and absence of relative arbitrage among all buy‐and‐hold strategies (called static efficiency). A valuation process for a payoff is then called semi‐efficient consistent if the financial market enlarged by that process still satisfies this combination of properties. It turns out that this approach lies in the middle between the extremes of valuing by risk‐neutral expectation and valuing by absence of arbitrage alone. We show that this always yields put‐call parity, although put and call values themselves can be nonunique, even for complete markets. We provide general formulas for put and call values in complete markets and show that these are symmetric and that both contain three terms in general. We also show that our approach recovers all the put‐call parity respecting valuation formulas in the classic theory as special cases, and we explain when and how the different terms in the put and call valuation formulas disappear or simplify. Along the way, we also define and characterize completeness for general semimartingale financial markets and connect this to the classic theory.
High-Throughput Protein Engineering Improves the Antigenicity and Stability of Soluble HIV-1 Envelope Glycoprotein SOSIP Trimers
Authors: Sullivan Jonathan T. , Sulli Chidananda , Nilo Alberto , Yasmeen Anila , Ozorowski Gabriel , Sanders Rogier W. , Ward Andrew B. , Klasse P. J. , Moore John P. , Doranz Benjamin J. 
Published: September 2017

ABSTRACT
Soluble envelope glycoprotein (Env) trimers (SOSIP.664 gp140) are attractive HIV-1 vaccine candidates, with structures that mimic the native membrane-bound Env spike (gp160). Since engineering trimers can be limited by the difficulty of rationally predicting beneficial mutations, here we used a more comprehensive mutagenesis approach with the goal of identifying trimer variants with improved antigenic and stability properties. We created 341 cysteine pairs at predicted points of stabilization throughout gp140, 149 proline residue substitutions at every residue of the gp41 ectodomain, and 362 space-filling residue substitutions at every hydrophobic and aromatic residue in gp140. The parental protein target, the clade B strain B41 SOSIP.664 gp140, does not bind the broadly neutralizing antibody PGT151 and so was used here to identify improved variants that also provide insight into the structural basis for Env antigenicity. Each of the 852 mutants was expressed in human cells and screened for antigenicity using four different monoclonal antibodies (MAbs), including PGT151. We identified 29 trimer variants with antigenic improvements derived from each of the three mutagenesis strategies. We selected four variants (Q203F, T538F, I548F, and M629P) for more comprehensive biochemical, structural, and antigenicity analyses. The T538F substitution had the most beneficial effect overall, including restoration of the PGT151 epitope. The improved B41 SOSIP.664 trimer variants identified here may be useful for vaccine and structural studies.

IMPORTANCE
Soluble Env trimers have become attractive HIV-1 vaccine candidates, but the prototype designs are capable of further improvement through protein engineering. Using a high-throughput screening technology (shotgun mutagenesis) to create and evaluate 852 variants, we were able to identify sequence changes that were beneficial to the antigenicity and stability of soluble trimers based on the clade B B41
env
gene. The strategies described here may be useful for identifying a wider range of antigenically and structurally improved soluble trimers based on multiple genotypes for use in programs intended to create a broadly protective HIV-1 vaccine.
Potent Plasmablast-Derived Antibodies Elicited by the National Institutes of Health Dengue Vaccine
Authors: Magnani Diogo M. , Silveira Cassia G. T. , Ricciardi Michael J. , Gonzalez-Nieto Lucas , Pedreño-Lopez Núria , Bailey Varian K. , Gutman Martin J. , Maxwell Helen S. , Domingues Aline , Costa Priscilla R. , Ferrari Lilian , Goulart Raphaella , Martins Mauricio A. , Martinez-Navio José M. , Fuchs Sebastian P. , Kalil Jorge , Timenetsky Maria do Carmo , Wrammert Jens , Whitehead Stephen S. , Burton Dennis R. , Desrosiers Ronald C. , Kallas Esper G. , Watkins David I. 
Published: September 2017

ABSTRACT

Exposure to dengue virus (DENV) is thought to elicit lifelong immunity, mediated by DENV-neutralizing antibodies (nAbs). However, Abs generated by primary infections confer serotype-specific protection, and immunity against other serotypes develops only after subsequent infections. Accordingly, the induction of these nAb responses acquired after serial DENV infections has been a long-sought-after goal for vaccination. Nonetheless, it is still unclear if tetravalent vaccines can elicit or recall nAbs. In this study, we have characterized the responses from a volunteer who had been previously exposed to DENV and was immunized with the live attenuated tetravalent vaccine Butantan-DV, developed by the NIH and Butantan Institute. Eleven days after vaccination, we observed an ∼70-fold expansion of the plasmablast population. We generated 21 monoclonal Abs (MAbs) from singly sorted plasmablasts. These MAbs were the result of clonal expansions and had significant levels of somatic hypermutation (SHM). Nineteen MAbs (90.5%) neutralized at least one DENV serotype at concentrations of 1 μg/ml or less; 6 of the 21 MAbs neutralized three or more serotypes. Despite the tetravalent composition of the vaccine, we observed a neutralization bias in the induced repertoire: DENV3 was targeted by 18 of the 19 neutralizing MAbs (nMAbs). Furthermore, the P3D05 nMAb neutralized DENV3 with extraordinary potency (concentration to achieve half-maximal neutralization [Neut
50
] = 0.03 μg/ml). Thus, the Butantan-DV vaccine engendered a mature, antigen-selected B cell repertoire. Our results suggest that preexisting responses elicited by a previous DENV3 infection were recalled by immunization.

IMPORTANCE
The dengue epidemic presents a global public health challenge that causes widespread economic burden and remains largely unchecked by existing control strategies. Successful control of the dengue epidemic will require effective prophylactic and therapeutic interventions. Several vaccine clinical efficacy trials are approaching completion, and the chances that one or more live attenuated tetravalent vaccines (LATVs) will be introduced worldwide is higher than ever. While it is widely accepted that dengue virus (DENV)-neutralizing antibody (nAb) titers are associated with protection, the Ab repertoire induced by LATVs remain uncharacterized. Here, we describe the isolation of potent (Neut
50
< 0.1 μg/ml) nAbs from a DENV-seropositive volunteer immunized with the tetravalent vaccine Butantan-DV, which is currently in phase III trials.
Association of admission and discharge anemia status with outcomes in patients hospitalized for acute decompensated heart failure: Differences between patients with preserved and reduced ejection fraction
Authors: Kajimoto Katsuya , Minami Yuichiro , Otsubo Shigeru , Sato Naoki 
Published: September 2017

Background: In acute decompensated heart failure patients with a preserved or reduced ejection fraction, the association of admission and discharge anemia status with outcomes remains unclear. Methods and results: Of the 4842 patients enrolled in the Acute Decompensated Heart Failure Syndromes (ATTEND) registry, 4433 patients (2017 with a preserved and 2416 with a reduced ejection fraction) were examined to investigate associations among the anemia status at admission and discharge (no anemia, developed anemia, resolved anemia, or persistent anemia), a preserved or reduced ejection fraction and the primary endpoint (all-cause death and readmission for heart failure). In the preserved ejection fraction group, adjusted analysis showed that either developed or persistent anemia was associated with a significantly higher risk of the primary endpoint relative to no anemia (hazard ratio: 1.53; 95% confidence interval (CI): 1.11–2.11; p=0.009 and hazard ratio: 1.60; 95% CI: 1.26–2.04; p<0.001, respectively), but there was no association between resolved anemia and the primary endpoint (hazard ratio: 0.98; 95% CI: 0.67–1.45; p=0.937). In the reduced ejection fraction group, either developed or resolved anemia was associated with a tendency toward higher risk of the primary endpoint relative to no anemia (hazard ratio: 1.29; 95% CI: 0.95–1.62; p=0.089, and hazard ratio: 1.31; 95% CI: 0.96–1.77; p=0.085, respectively), while persistent anemia was associated with a significantly higher risk of the primary endpoint relative to no anemia (hazard ratio: 1.36; 95% CI: 1.12–1.65; p=0.002). Conclusions: In acute decompensated heart failure patients, the association of admission and discharge anemia status with outcomes differs markedly between patients with a preserved or reduced ejection fraction.
Long-Term Economic and Employment Outcomes Among Partners of Women With Early-Stage Breast Cancer
Authors: Veenstra Christine M. , Wallner Lauren P. , Jagsi Reshma , Abrahamse Paul , Griggs Jennifer J. , Bradley Cathy J. , Hawley Sarah T. 
Published: September 2017

Purpose: Work loss is one of many personal costs for patients with cancer and their families. Many women with breast cancer face long-term job loss that stems from their diagnoses. However, little is known about the economic and employment outcomes of partners of women with breast cancer. Methods: Women with nonmetastatic breast cancer identified by the Detroit and Los Angeles SEER registries between June 2005 and February 2007 were surveyed at both 9 months and 4 years after diagnosis. Partners were surveyed 4 years after patients’ diagnoses. Nonretired partners were asked about employment and financial consequences that stemmed from the patients’ breast cancer diagnoses and treatments. Results: A total of 517 (67%) of 774 eligible partners completed the survey; 32% reported worsened financial status attributed to patients’ breast cancers. Two hundred forty nonretired partners worked during the year after the patients’ diagnoses; 90% were still working 4 years postdiagnosis. A total of 32% of partners decreased their work hours as a result of patients’ breast cancer diagnoses and treatments; 64% of partners reported that, as a result of patients’ breast cancer diagnoses and treatments, it was very/extremely important to keep working to keep health insurance. Overall, 51% of partners reported that it was very/extremely important to avoid changing jobs, because they were worried about loss of health insurance. Conclusion: Nearly one third of partners reported that their financial status was worse because of the patient’s breast cancer, although most remained working 4 years after a diagnosis. Partners may continue to work longer than desired to compensate for a loss of financial resources in the family.
Medikamentenbezogene Überzeugungen („beliefs about medicines“): Konzept und Rolle in Public Health
Authors: Brandstetter Susanne , Apfelbacher Christian 
Published: September 2017

Zusammenfassung
Non-Adhärenz bzgl. der verordneten Medikation ist ein ubiquitäres Phänomen in der Versorgung chronischer Krankheiten. Unter den Adhärenz beeinflussenden Faktoren wurden in jüngerer Zeit vermehrt sog. medikamentenbezogene Überzeugungen betrachtet. Über eine Vielzahl von Studien hinweg zeigt sich Adhärenz konsistent mit stärker ausgeprägten Überzeugungen zur Notwendigkeit der eigenen Medikation und weniger stark ausgeprägten Befürchtungen assoziiert. Die Evidenzlage zu Interventionen ist unklar.
Microstructure and corrosion properties of SiC/Al-Mg-Cu-Si-Sn composites
Authors: Shen Rujuan , Zhou Pengfei , Xiao Daihong , Song Min 
Published: September 2017

Abstract
The SiC/Al-Mg-Cu-Si-Sn composites with different quantities of SiC particles by volume were fabricated using hot-pressing sintering. The effects of SiC volume fraction on the microstructure, Vickers hardness, and corrosion behavior of the composites were studied using the optical microscope, scanning electron microscope, Vickers hardness test, and corrosion testing. The results show that adding SiC particles shortened the time required to reach the peak-aged state of the composites and increased the hardness. Corrosion potentials of the composites did not vary greatly or show any definite trends in relation to the amounts of the SiC contents.
Elternschaft aus theologisch-ethischer Perspektive vor dem Hintergrund des EKD-Familienpapiers
Authors: Schardien Stefanie 
Published: September 2017

Zusammenfassung:
Die evangelische theologisch-ethische und kirchliche Perspektive auf Elternschaft hat sich in den vergangenen Jahren deutlich verändert. Anstatt auf die jeweilige Gestalt von Familie und die damit verbundenen Formen von Elternschaft zu fokussieren, ist die Gestaltung der Beziehungen deutlicher in den Blick gerückt. Der Artikel beschreibt die Entwicklung und theologische Motivation dieser Veränderungen und skizziert die möglichen gemeindepraktischen Konsequenzen in der heutigen Elternarbeit.
Religiöse Bildung und Elternarbeit im schulischen Kontext
Authors: Domsgen Michael 
Published: September 2017

Zusammenfassung:
In jüngerer Zeit rückt der Zusammenhang zwischen schulischer Bildung und familialer Sozialisation verstärkt in das Blickfeld des Interesses. Nicht zuletzt die PISA-Studien zeigen, dass gegenwärtige Unterrichtsformen und -stile nicht für alle Schülerinnen und Schüler gleichermaßen anschlussfähig sind. Religiöse Bildung ist davon nicht ausgenommen, wie ein Blick (hier am Beispiel Sachsen-Anhalts) auf ausgewählte empirische Befunde zeigen kann. Das lässt verstärkt nach der Einbeziehung der Familienperspektive fragen. Entfaltet wird das einerseits hinsichtlich ihrer religionsdidaktischen Implikationen und andererseits mit Blick auf Anforderungen einer religionspädagogisch profilierten Elternarbeit.
PEMELIHARAAN LARVA KERAPU RAJA SUNU (Plectropomus laevis) DENGAN PERBEDAAN AWAL PEMBERIAN PAKAN BUATAN
Authors: Slamet Bejo , Aslianti Titiek , Setiawati Ketut Maha , Andriyanto Wawan , Nasukha Afifah 
Published: September 2017

Ikan kerapu raja sunu (Plectropomus laevis) merupakan komoditas ekspor yang bernilai ekonomis tinggi di pasar Asia, namun stoknya di alam sudah langka. Pemberian pakan buatan dalam pemeliharaan larvanya telah dilakukan, namun waktu respon awal larva terhadap pakan buatan secara tepat belum diketahui. Penelitian ini bertujuan untuk mengetahui waktu awal pemberian pakan buatan yang efektif pada pemeliharaan larvanya. Tiga perlakuan pemberian pakan buatan yang diuji yaitu mulai umur 7 hari (D-7) (A), D-10 (B), dan D-13 (C). Parameter yang diamati meliputi perkembangan panjang total, sintasan larva, jumlah rotifer dalam lambung dan histologi organ pencernaan larva. Penelitian berlangsung sampai larva berumur 43 hari, menggunakan rancangan acak kelompok (RAK) dengan tiga kelompok waktu untuk setiap perlakuan. Hasil penelitian menunjukkan bahwa perbedaan waktu awal pemberian pakan buatan berpengaruh terhadap sintasan dan pertumbuhan larva. Panjang total dan sintasan larva umur 43 hari yang tertinggi dicapai pada perlakuan B yaitu 29,3 mm ± 0,36 mm dan 2,61% ± 0,21%; diikuti oleh perlakuan C dengan nilai 28,0 mm ± 0,85 mm dan 1,55% ± 0,18%; dan yang terendah pada perlakuan A adalah 27,0 mm ± 0,25 mm dan 0,85% ± 0,13%. Data ini menunjukkan bahwa waktu awal pemberian pakan buatan yang terbaik adalah mulai larva berumur 10 hari.
PENGARUH MEKANISME CORPORATE GOVERNANCE DAN UMUR PERUSAHAAN TERHADAP KETEPATAN WAKTU PELAPORAN KEUANGAN (Studi Empiris Pada Perusahaan Sektor Pertambangan yang Terdaftar di Bursa Efek Indonesia Pada Tahun 2010-2012)
Authors: Bulo Randi Hermawan , Arafat M. Yasser , Anggraini Ratna 
Published: September 2017

The objective of study examines the influence of mechanism corporate governance consist of managerial ownership and institutional ownership, and then age of company on timeliness of corporate financial reporting. This research represents the empirical test which used purposive sampling techniques in data collection. Data were collected using a secondary data of 28 from mining company listed in Indonesian Stock Exchange 2010-2012. Data analysis uses multiple regression with the program SPSS 16.00 version for windows. Results of hypothesis examination indicate there is influence between institutional ownership and age of company on Timeliness of Corporate Financial Reporting, and there is no influence between Managerial ownership on Timeliness of Corporate Financial Reporting.

Keywords: Timeliness, Managerial Ownership, Institutional Ownership, Age of Company
Estimation of Thermal Instabilities in Soils around Underground Electrical Power Cables
Authors: Kroener Eva , Campbell Gaylon S. , Bittelli Marco 
Published: September 2017

Core Ideas
We studied coupled water, vapor, and heat flow around underground cables.
There is a water–vapor flow cycle in the soil around these cables.
This water–vapor cycle breaks down at the critical heat dissipation rate.
The critical heat dissipation rate depends on soil hydraulic properties.
The decentralized production of electrical energy from wind requires the installation of more and more underground power cables. From an economic standpoint it is very important to estimate the cables' lifetime, which strongly depends on the temperature. A correct estimation of cable temperature requires an accurate description of the heat balance equation around the cable. Most of the models used to assess heat dissipation from cables, however, do not consider that the soil thermal conductivity strongly depends on soil water content and hydraulic dynamics in the vicinity of the cable. The high temperature around the cable induces a water–vapor cycle in the soil: liquid water evaporates near the cable, and this vapor condensates in the more distant and colder parts of the soil. Above a critical heat dissipation rate, the soil around the cable dries out and the water–vapor cycle breaks down. In this study, we analytically estimated the critical heat dissipation rate as a function of soil type, soil temperature, and water content. We validated the analytical estimation by comparison to a numerical solution of the coupled heat–water–vapor transport for various soil types and conditions. The numerical code was validated by simulating a soil drying experiment. The proposed estimation of thermal instabilities in soils could become a powerful tool in the design of underground electrical power cable systems.
Optimización del proceso de elaboración del bollo de yuca en el municipio de San Juan Nepomuceno, Bolívar-Colombia
Authors: Granados Conde Clemente , Sarabia Caffroni Estefanía , Granados Llamas Edgar 
Published: September 2017

El objetivo de ésta investigación fue optimizar el proceso de elaboración del bollo de yuca, el cual es un producto autóctono de la costa caribe colombiana y se procesa en el municipio de San Juan de Nepomuceno, departamento de Bolívar, donde se evaluó el proceso actual y el propuesto con el fin de verificar su calidad, aceptación, estudio de tiempos y movimientos. Los resultados obtenidos establecieron que ambos bollos presentaron un nivel de calidad microbiológica de acuerdo a lo establecido en la NTC 4519; el proceso propuesto presentó un menor recuento microbiano debido a que se estableció control higiénico sanitario. En lo referente a la evaluación sensorial el proceso propuesto favoreció la aceptabilidad del bollo de yuca, con altos valores de olor, sabor y color respectivamente, la textura del bollo de yuca artesanal fue el de mayor aceptación; en el aspecto fisicoquímico el bollo de yuca propuesto presentó mejores características como un incremento en proteínas y fibras. En el estudio de tiempos y movimientos, estos se disminuyeron un 24.4% en tiempo y un 33 % en movimientos, los cuales optimizaran el proceso y disminuirán los costos.
DOSIMETRÍA IN VIVO EN TRATAMIENTOS DE CANCER DE CÉRVIX CON Ir-192
Authors: Alvarino Gustavo B , Pardo P Enrique , Quijano Parra Alfonso 
Published: September 2017

Para asegurar la calidad en los tratamientos de braquiterapia de alta tasa de dosis intracavitarios en Ca de cérvix, es necesario que los fotones de Ir – 192 entreguen la dosis prescrita, con la mayor precisión posible, en el tumor y se debe minimizar la distribución de dosis, en el tejido sano y órganos críticos que rodean al tumor, tan baja como sea razonablemente deseable. Por esta razón debemos hacer la medición directamente sobre el tejido humano. En este trabajo se realiza el proceso de medición en pacientes con cáncer de cérvix tratados con Ir –192, en los cuales utilizamos dosímetros TLD-100 de LiF: Mg; Ti, que se ubican en recto y vejiga para comprobar la dosis real recibida, utilizando la dosimetría in vivo, comparándola con la medición arrojada por el sistema de planeación Abacus 3.1, puesto que estos órganos deben recibir menos del 70%. Palabras clave: Braquiterapia HDR, Dosimetría in vivo, Termoluminiscencia