bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex disposition of an enigmatic pathogen: rare electron microscopic manifestations in nasal rhinosporidiosis
Authors: Dhaulakhandi Dhara B. , Ravi Alok K. , Garg Lalit C. , Ahluwalia Karvita B. 
Published: August 2017

Abstract
Rhinosporidiosis is a polypoidal disease of the nose and mucocutaneous tissues, the diagnosis of which is based on the presence of round bodies believed to be causative agents of the disease. Historically, the round body has been considered to be a sporangium of a fungus Rhinosporidium seeberi but without any convincing evidence. Round bodies contain numerous daughter cells, which are likely in the infective stage and are shed through a rupture in the wall of the round body. The released single-celled organisms eventually develop into round bodies on availability of suitable transformative trigger and favourable environment. Surgical excision of the polyp by electrocautery is the only effective treatment; however, recurrence may occur due to spillage of infective endospores in the surrounding mucosa during removal. There are many enigmatic features of the causative agent of this disease, which have been baffling researchers for more than a century. Here we present some rare electron microscopic and previously unreported features of the coat of the round body and single-celled organism in nasal rhinosporidiosis.
Genetic and Hormonal Determinations in a Pair of Identical Twins with Early Onset Psoriasis Vulgaris: Case Report and a Brief Review of the Literature
Authors: Roman Iulia Ioana , Orasan Meda Sandra , Coneac Andrei , Marina Elena Mihaela , Pop Alexandra Dana , Orasan Remus Ioan 
Published: August 2017

Abstract
Psoriasis vulgaris is a chronic inflammatory dermatosis with major impact on patients’ life quality. The etiopathogenesis is multifactorial, depending on complex interactions between genetic and environmental factors. We present the case of two female patients, identical twins of 33 years old, suffering from psoriasis vulgaris since childhood. Patient A developed specific lesions of psoriasis at the age of 7 and patient B started to develop psoriasis lesions on the scalp two years later. At the age of 31, patient A was diagnosed with psoriatic arthritis. Laboratory test results were within the normal ranges for both patients. Hormonal and immunological determinations revealed the presence of a high level of antithyroidperoxidase antibody in patient A and increased level of prolactin in patient B. Ultrasonographic assessment of the thyroid detected the presence of bilateral micronodules in the first subject. Knowing that early onset psoriasis is associated with the presence of Human Leukocyte Antigen Cw6(HLA-Cw6), we aimed to confirm this hypothesis for our subjects. Although HLA-Cw6 is the most frequent mutation in psoriasis patients and it is present in about two-thirds of the tested subjects,the genetic results for both patients were negative, strengthening the fact that other factors, the environmental one and the hormonal disorders had an important role in their psoriasis pathogenesis. Under these conditions, we emphasize the importance of including a hormonal evaluation approach of psoriasis patients in order to diagnose and treat pathologies that may be related with disease exacerbations
Gene exchange across a postglacial contact zone in Fraxinus excelsior L.
Authors: Gömöry D. , Paule L. , Krajmerová D. , Romšáková I. , Piecka J. 
Published: August 2017

AbstractHybridization between divergent lineages of common ash Fraxinus excelsior L. was studied in Slovakia and adjacent regions of Hungary, the Czech Republic and Poland in the contact zone between two postglacial colonization routes originating from different glacial refugia. Thirty-eight common ash populations were studied using a combination of 6 maternally inherited chloroplast microsatellite markers and 7 biparentally inherited nuclear microsatellites (nSSR). Two predominant chloroplast lineages were identified, between which the boundary was very sharp. Populations containing a mixture of different haplotypes were found only in the immediate proximity of the boundary. Bayesian analysis of population structure based on nSSR loci revealed the existence of two clusters, which largely coincided with chloroplast lineages. Both haplotype frequencies and proportions of clusters identified by the Bayesian analysis exhibited a clinal transition over the hybrid zone, with cline widths of 36 km for chloroplast haplotype frequencies (reflecting gene flow by seeds) and 275 km for Bayesian clusters based on nSSR (reflecting gene flow by pollen and seeds). Chloroplast haplotype diversity increased along the boundary between lineages not only because of admixture, but also due to the presence of rare haplotypes. In contrast, diversity at nuclear loci did not exhibit any geographical trend.
Pollination methods, stigma receptivity and pollen tube growth in Eucalyptus argophloia
Authors: Randall Bruce W. , Walton David A. , Lee David J. , Wallace Helen M. 
Published: August 2017

Abstract
Eucalyptus argophloia Blakely (Western white gum) has shown potential as a commercial forestry timber species in marginal environments of north-eastern Australia. We measured early pollination success in Eucalyptus argophloia to compare pollination methods, determine the timing of stigma receptivity and compare fresh and stored pollen. Early pollination success was measured by counting pollen tubes in the style of E. argophloia 12 days after pollination. We compared the early pollination success of 1) Artificially Induced Protogyny (AIP), one-stop and three-stop methods of pollination; 2) flowers pollinated at 2 day intervals between 2 days before and 6 days after anthesis and 3) fresh pollen and pollen that had been stored for 9 months. Our results show significantly more pollen tubes from unpollinated AIP and AIP treatments than either the one-stop pollination or three-stop pollination treatments. This indicates that self-pollination occurs in the unpollinated AIP treatment. There was very little pollen tube growth in the one-stop method indicating that the three-stop method is the most suitable for this species. Stigma receptivity in E. argophloia commenced six days after anthesis and no pollen tube growth was observed prior to this. Fresh pollen resulted in pollen tube growth in the style whereas the stored pollen resulted in a total absence of pollen tube growth. We recommend that breeding programs incorporating E. argophloia as a female parent use the three-stop pollination method, and controlled pollination be carried out at least six days after anthesis using fresh pollen.
Microarray Analysis of Gene Expression in Triploid Black Poplar
Authors: Hu Baoquan , Wang Bin , Wang Chunguo , Song Wenqin , Chen Chengbin 
Published: August 2017

Abstract
Triploidy is a widespread phenomenon in cultivated and natural breeding plants and it can confer some growth advantages. Here, we analyzed genome-wide gene expression in triploid Populus euramericana (black poplar) using the Affymetrix poplar microarray to detect any possible correlation between triploid vigor and a unique gene expression profile. Among the 38,400 transcripts that were detected in triploid poplar, 1,564 and 2,015 genes were up- or downregulated, respectively, compared with the diploid. The majority of the upregulated genes in the triploid were associated with carbon and nitrogen metabolism, especially lignin and secondary metabolism. Other genes upregulated in the triploid included genes involved in sugar transport, and brassinosteroid (BR) and auxin metabolism. Downregulated genes were mostly related to the assembly and biosynthesis of ribosomes and the nucleosome macromolecular complex. The results suggested that BR and auxin levels were crucial in controlling sugar transport, photosynthesis and cell wall biosynthesis. Downregulated genes were associated with chromatin regulation in the triploid. The information from this analysis could provide an insight into the vigor of triploid poplar.
Genetic Control of Growth Traits and Inheritance of Resistance to Bacterial Leaf Scorch in American Sycamore
Authors: Adams J. P. , Rousseau R. J. , Leininger T. D. 
Published: August 2017

AbstractOpen-pollinated progeny tests of American sycamore (Platanus occidentalis L.), which included 55 open-pollinated families selected from several prior Westva - co progeny tests and seed orchards and six control-pollinated families were established in 2002 and 2003. The half-sibling families were planted at two sites in western Kentucky and southeastern Missouri. The six full-sibling families, generated from selections based on exhibition of parental disease resistance and susceptibility to a variety of diseases, were also planted near Stoneville, MS at the US Forest Service Center for Bottomland Hardwoods Research. All full-sibling families planted at the Stoneville site were inoculated in the fall 2002 with the leaf-scorch-causing bacterium, Xylella fastidiosa. Diameter and height data for trees of both half- and full-sibling families were recorded at ages three, five, seven, and nine at the various sites. Bacterial leaf scorch disease presence was also recorded as symptomatic or asymptomatic/ mildly symptomatic. Average family heritability across ages was 0.58, 0.50, and 0.55 for height, diameter, and volume, respectively, indicating that increased growth traits can be gained from family selection. In conjunction with derived age-age correlations, selection based on age-five data results in the greatest gain per unit time for age nine gains for half-sibling families. Breeding for bacterial leaf scorch resistance can also be successfully undertaken if proper selection and breeding of two resistant parents are undertaken which can result in a 4.5 fold decrease in the probability in symptoms of offspring by age nine. Results indicate a large potential for increased growth and disease resistance in American sycamore through traditional breeding.
Statistical associations between morphology, physiology and AFLP DNA markers enable selection of a putative eucalypt hybrid able to tolerate salt affected floodplains
Authors: Koerber G. R. , Hancock T. 
Published: August 2017

AbstractA naturally occurring putative hybrid between Eucalyptus largiflorens F. Muell and Eucalyptus gracilis F. Muell called Green Box tolerates saline conditions of the River Murray floodplains better than E. largiflorens. Revegetation strategies utilizing seedlings of Green Box have had limited success because only a few are Green Box and the majority are throw backs to E. gracilis and E. largiflorens. Therefore, the purpose of this study was to identify traits characteristic of Green Box and AFLP markers associated with the traits enabling selection at the seedling stage. This was done by non-linear canonical correlation analysis (OVERALS) to test for statistically significant associations between morphological and physiological traits with 232 AFLP markers from 9 primer combinations. OVERALS with all markers produced 1stand 2nddimensions accounting for 80 and 74% of variation respectively. Green Box plants were placed intermediate between E. gracillis and E. largiflorens according to leaf colour, gloss and nitrogen with component loadings (lc) of 0.340, 0.615 and 0.294 respectively. A second approach of simple linear regression of morphological and physiological traits against all 232 AFLP markers singled out 17 with significance P<0.05. Thirteen of these were also identified by OVERALS. Four occurred with high frequency in Green Box and E. largiflorens distinguishing them from E. gracilis. In order to separate Green Box and E. largiflorens, the segregation of a further three markers can be used to align Green Box with E. gracilis. Therefore, the segregation of 7 markers can be utilized to select Green Box.
Evaluating Interior Spruce Seed Deployment with GIS-Based Modeling Using British Columbia’s Prince George Seed Planning Zone as a Model
Authors: Ding C. , Mcauley L. , Meitner M. J. , El-Kassaby Y. A. 
Published: August 2017

Abstract
The maintenance, protection, and conservation of forest genetic resources for economic, ecological and social benefits are daunting tasks. Understanding how reforestation materials are spatially and temporally deployed across the landscape is an integral component of forest genetic resources management. To improve the current understanding of how reforestation materials are deployed in British Columbia (BC), we developed a geographical information systems (GIS) method to track seed deployment across silviculture openings. Generally, reforestation materials can originate from either natural stand (wild seed collections) or orchards’ seed sources (improved seed); the latter are produced within the framework of specific tree improvement program designed for a particular species within a well-defined seed deployment area, commonly known as Seed Planning Zone (SPZ). In this paper, we present a GIS-based method for evaluating seed deployment patterns for interior spruce (Picea glauca and Picea engelmannii and their natural hybrids) within the Prince George SPZ. The evaluation period (1970-2004) is associated with wild stands and improved seed availability and the dynamic of each seed source proportionate contribution followed three distinct phases; namely, developing (1970-1987), immature (1988-1994), and mature (1995-2004) with a progressive increase of orchards’ seed use over time. The developed method is scalable across SPZs of the same species or multiple species, thus providing the means to: 1) temporally and spatially monitor improved and natural stands seed deployment over the landscape; and 2) identify areas of concerns where a particular seed source is over-represented which might pose an increased genetic vulnerability. The present study revealed that the current interior spruce orchard’s seed use within the Prince George SPZ is expected to exceed the provincial goal of performance target of 75% by 2014. Additionally, areas of excessive use of one seed orchard seed were identified.
Analysis of Energy Consumption of Crushing Processes – Comparison of One-Stage and Two-Stage Processes
Authors: Ciężkowski Paweł , Maciejewski Jan , Bąk Sebastian 
Published: August 2017

Abstract
This paper presents experimental comparison of two machine crushing technologies: one-stage and two-stage. The study was carried on a model double-toggle jaw crusher which allows crushing forces, energy and toggle displacement to be measured. The main aim of the work was to determine the energy consumption of crushing process assuming a given level of fragmentation. Studies were performed on three rocks: granite “Strzegom”, limestone “Morawica” and sandstone “Mucharz”. The material tested had a cubic shape and average dimension of 90 mm. One-stage crushing was carried out for outlet slot er = 11 mm, and two-stage crushing for er = 24 mm and 11 mm. In the tests special design of variable profile moving jaw was used and fixed jaw was flat. The analysis of the results shows that taking into account energy consumption, it is better to use two-stage crushing process. For given materials energy consumption in the two-stage crushing process was reduced by 30%.
Clinical development of CAR T cells—challenges and opportunities in translating innovative treatment concepts
Authors: Hartmann Jessica , Schüßler‐Lenz Martina , Bondanza Attilio , Buchholz Christian J 
Published: August 2017

AbstractChimeric antigen receptor (CAR) T cell therapy, together with checkpoint inhibition, has been celebrated as a breakthrough technology due to the substantial benefit observed in clinical trials with patients suffering from relapsed or refractory B‐cell malignancies. In this review, we provide a comprehensive overview of the clinical trials performed so far worldwide and analyze parameters such as targeted antigen and indication, CAR molecular design, CAR T cell manufacturing, anti‐tumor activities, and related toxicities. More than 200 CAR T cell clinical trials have been initiated so far, most of which aim to treat lymphoma or leukemia patients using CD19‐specific CARs. An increasing number of studies address solid tumors as well. Notably, not all clinical trials conducted so far have shown promising results. Indeed, in a few patients CAR T cell therapy resulted in severe adverse events with fatal outcome. Of note, less than 10% of the ongoing CAR T cell clinical trials are performed in Europe. Taking lead from our analysis, we discuss the problems and general hurdles preventing efficient clinical development of CAR T cells as well as opportunities, with a special focus on the European stage.
Attitudes of Consumers Towards Islamic and Conventional Credit Cards in Indonesia
Authors: Rusmita Sylva Alif , Ajija Shochrul Rohmatul 
Published: August 2017

This study aims to analyze the attitudes of consumers towards Islamic and conventional credit cards. Using online questionnaire survey data from 51 respondents in Surabaya, East Java, the study revealed that most consumers possessed credit cards because of their convenience factor, relationship with their existing bank, and card salesmen. Therefore, the sale is the most powerful way to invite the community to have an Islamic credit card. Many customers do not care whether their credit cards are Islamic based or not, as long as the salesman promoted cards to them and the cards are able to meet their personal needs, especially for sales and purchase transactions online, they will utilize the cards. The large number of Muslims in Surabaya should be a share of the lucrative market for Islamic credit cards. Therefore, the education about the Islamic manner of consumption and the dangers of usury should be promoted in Surabaya.DOI: 10.15408/etk.v16i2.5519
Dinámica de la epidemia de malaria en Colombia: Predicción probabilística temporal
Authors: Rodríguez Javier , Prieto-Bohórquez Signed E. , Correa-Herrera Sandra C. , Pérez-Díaz Carlos E. , Soracipa-Muñoz María Y. 
Published: August 2017

Objetivo Realizar una predicción de la dinámica de la epidemia de malaria para el 2007 en Colombia con base en el análisis de la dinámica geométrica de 1960-2006 como una caminata al azar probabilista.Materiales y Métodos Basados en la caminata al azar probabilística se estudió la dinámica geométrica del número de casos anuales de malaria registrados en Colombia durante los años 1960-2006, analizando el comportamiento probabilístico de aumentos y disminuciones consecutivos, y el comportamiento probabilístico de casos durante rangos de años consecutivos, para así realizar una predicción temporal de los casos.Resultados Se desarrolló una metodología sencilla y acausal que predice los valores extremos 81 003 y 104 098 para el número de infectados en el año 2007, predicción que fue refinada con el análisis de las variaciones anuales obteniendo un valor de 104 098 para el número de infectados en el año 2007. Esta predicción fue posteriormente corroborada con los datos del Instituto Nacional de Salud de Colombia, correspondiendo al 95,6 % respecto al número de casos reportados.Conclusión La comprensión del fenómeno acausal a partir de la caminata al azar probabilística permite realizar predicciones temporales, simples y prácticas, directamente comprobables y aplicables, economizando tiempo y recursos.
Cumprimento de metas dos Centros de Especialidades Odontológicas da Região Sul do Brasil.
Authors: Moura Flavio Renato Reis de , Tovo Maximiliano Ferreira , Celeste Roger Keller 
Published: August 2017

Objetivo El objetivo del estudio fue verificar: el cumplimento de las metas de tratamientos en las clínicas dentales especializadas (CDEs) en la Región Sur de Brasil y, los factores asociados a los procesos de trabajo.Método El estudio fue exploratorio transversal. Para la recolección de datos fueron consultadas las bases del datos provenientes de DATA/SUS, CNES, SIOSP, IBGE y fue enviado un cuestionario semiestructurado para los gestores de las CDEs. Para comprobar la asociación de los factores con el cumplimento de las metas de las CDEs se realizó el test de Kruskal Wallis.Resultados Fueron analizados 936 meses de tratamiento ambulatorio distribuidos en las 78 CDEs y la tasa de respuestas de los cuestionarios fue de 68%. Las variables: estado de Paraná, municipios con número de ciudadanos de 0-50 mil/hab, el cuarto quintil de los gastos totales en salud y PIB per cápita fueron asociados con el cumplimento de las metas de los procedimientos de periodoncia y, el tipo II de CDE presentó asociación con el cumplimento de las metas de los procedimientos básicos.Discusión El análisis de las variables que pueden estar asociadas al tratamiento ambulatorio en las CDEs es fundamental para comprobar tanto los problemas de acceso de los habitantes como la aplicabilidad de los recursos públicos en el área de la salud.Conclusiones El estudio indica un bajo cumplimento de las metas de tratamiento de las CDEs, entretanto apunta que pueden haber factores importantes asociados al mayor número de meses en las metas cumplidas.
Teoria de Utilidade Epistêmica e o Argumento da Acurácia a favor do Probabilismo
Authors: Neiva André 
Published: August 2017

Diferente dos argumentos tradicionais na literatura em epistemologia formal, o argumento da acurácia gradacional de James Joyce fornece uma justificação epistêmica do probabilismo. O seu argumento pretende mostrar que funções probabilísticas de graus de crença têm maior grau de acurácia, ou menor grau de inacurácia, do que qualquer outra função não probabilística de crença gradual. Neste artigo, reconstruímos e examinamos a sua proposta, que é definida como uma estratégia de utilidade epistêmica. Também fornecemos uma aplicação de uma determinada medida de inacurácia (Brier score) sobre diferentes funções de crença gradual. Por fim, discutimos alguns problemas que foram levantados contra a versão original do argumento de Joyce e concluímos com algumas sugestões de resposta a esses problemas.
A Review of Von Hippel-Lindau Syndrome
Authors: Varshney Neha , Kebede Amanuel A. , Owusu-Dapaah Harry , Lather Jason , Kaushik Manu , Bhullar Jasneet S. 
Published: August 2017

Von Hippel-Lindau syndrome (VHL) is a familial neoplastic condition seen in approximately 1 in 36,000 live births. It is caused by germline mutations of the tumor suppressor gene VHL, located on the short arm of chromosome 3. While the majority of the affected individuals have a positive family history, up to 20% of cases arise from de novo mutations. VHL syndrome is characterized by the presence of benign and malignant tumors affecting the central nervous system, kidneys, adrenals, pancreas, and reproductive organs. Common manifestations include hemangioblastomas of the brain, spinal cord, and retina; pheochromocytoma and paraganglioma; renal cell carcinoma; pancreatic cysts and neuroendocrine tumors; and endolymphatic sac tumors. Diagnosis of VHL is prompted by clinical suspicion and confirmed by molecular testing. Management of VHL patients is complex and multidisciplinary. Routine genetic testing and surveillance using various diagnostic techniques are used to help monitor disease progression and implement treatment options. Despite recent advances in clinical diagnosis and management, life expectancy for VHL patients remains low at 40–52 years. This article provides an overview of the major clinical, histological, and radiological findings, as well as treatment modalities.