bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of a Hypermucoviscous Extended-Spectrum-β-Lactamase-Producing
Klebsiella quasipneumoniae
subsp.
similipneumoniae
Clinical Isolate
Authors: Garza-Ramos U. , Silva-Sánchez J. , Catalán-Nájera J. , Barrios H. , Rodríguez-Medina N. , Garza-González E. , Cevallos M. A. , Lozano L. 
Published: July 2016

ABSTRACT

A clinical isolate of extended-spectrum-β-lactamase-producing
Klebsiella quasipneumoniae
subsp.
similipneumoniae
06-219 with hypermucoviscosity phenotypes obtained from a urine culture of an adult patient was used for whole-genome sequencing. Here, we report the draft genome sequences of this strain, consisting of 53 contigs with an ~5.6-Mb genome size and an average G+C content of 57.36%. The annotation revealed 6,622 coding DNA sequences and 77 tRNA genes.
Genome Sequence of
Gordonia
Phage Yvonnetastic
Authors: Pope Welkin H. , Bandyopadhyay Anshika , Carlton Meghan L. , Kane Meghan T. , Panchal Niyati J. , Pham Yvonne C. , Reynolds Zachary J. , Sapienza Michael S. , German Brian A. , McDonnell Jill E. , Schafer Claire E. , Yu Victor J. , Furbee Emily C. , Grubb Sarah R. , Warner Marcie H. , Montgomery Matthew T. , Garlena Rebecca A. , Russell Daniel A. , Jacobs-Sera Deborah , Hatfull Graham F. 
Published: July 2016

ABSTRACT

Gordonia
bacteriophage Yvonnetastic was isolated from soil in Pittsburgh, PA, using
Gordonia terrae
3612 as a host. Yvonnetastic has siphoviral morphology and a genome of 98,136 bp, with 198 predicted protein-coding genes and five tRNA genes. Yvonnetastic does not share substantial sequence similarity with other sequenced bacteriophage genomes.
Draft Genome Sequence of the Endophytic Strain
Rhodococcus kyotonensis
KB10, a Potential Biodegrading and Antibacterial Bacterium Isolated from
Arabidopsis thaliana
Authors: Hong Chi Eun , Jo Sung Hee , Jeong Haeyoung , Park Jeong Mee 
Published: July 2016

ABSTRACT

Rhodococcus kyotonensis
KB10 is an endophytic bacterium isolated from
Arabidopsis thaliana
. The organism showed mild antibacterial activity against the phytopathogen
Pseudomonas syringae
pv. tomato DC3000. This study reports the genome sequence of
R. kyotonensis
KB10. This bacterium contains an ectoine biosynthesis gene cluster and has the potential to degrade nitroaromatic compounds. The identified bacterium may be a suitable biocontrol agent and degrader of environmental pollutants.
Draft Genome Sequence of the Causative Antigen of Summer-Type Hypersensitivity Pneumonitis,
Trichosporon domesticum
JCM 9580
Authors: Cho Otomi , Ichikawa Tomoe , Kurakado Sanae , Takashima Masako , Manabe Ri-ichiroh , Ohkuma Moriya , Sugita Takashi 
Published: July 2016

ABSTRACT

Here, we report the draft genome sequence of
Trichosporon domesticum
JCM 9580, isolated from the house of a patient with summer-type hypersensitivity pneumonitis (SHP) in Japan. This genomic information will help elucidate the mechanisms of the development of SHP.
Draft Genome Sequence of “
Candidatus
Mycoplasma haemobos,” a Hemotropic Mycoplasma Identified in Cattle in Mexico
Authors: Martínez-Ocampo Fernando , Rodríguez-Camarillo Sergio D. , Amaro-Estrada Itzel , Quiroz-Castañeda Rosa Estela 
Published: July 2016

ABSTRACT

We present here the draft genome sequence of the first “
Candidatus
Mycoplasma haemobos” strain found in cattle in Mexico. This hemotropic mycoplasma causes acute and chronic disease in animals. This genome is a starting point for studying the role of this mycoplasma in coinfections and synergistic mechanisms associated with the disease.
Whole-Genome Sequencing of Two
Bartonella bacilliformis
Strains
Authors: Guillen Yolanda , Casadellà Maria , García-de-la-Guarda Ruth , Espinoza-Culupú Abraham , Paredes Roger , Ruiz Joaquim , Noguera-Julian Marc 
Published: July 2016

ABSTRACT

Bartonella bacilliformis
is the causative agent of Carrion's disease, a highly endemic human bartonellosis in Peru. We performed a whole-genome assembly of two
B. bacilliformis
strains isolated from the blood of infected patients in the acute phase of Carrion's disease from the Cusco and Piura regions in Peru.
Acupuncture for Chronic Central Serous Chorioretinopathy: A Prospective Case Series
Authors: Liang Zhu , Tian Chenbin 
Published: July 2016

Objective The aim of this prospective observational study was to evaluate the effects of acupuncture at Erjian on chronic central serous chorioretinopathy (CSC). Study Design Prospective case series. Methods Nine patients with chronic CSC were recruited and each patient received 12 sessions of acupuncture treatment. Subjective symptom measures included complications, best-corrected visual acuity (BCVA), fluorescein leakage (via fluorescein fundus angiography), and central subfield foveal subretinal fluid (on optical coherence tomography (OCT)) at 3 months. Successful treatment was defined as the complete resolution of subretinal fluid observed by the OCT following completion of treatment. Results After 2 months of treatment, two patients showed improvements in visual acuity, a significant reduction in subretinal fluid and an improvement in BCVA to 0.5±0.10 (mean±SD). By contrast, four patients experienced no significant changes. After 3 months of treatment, four patients showed improvements in visual acuity and complete resolution of the subretinal fluid (mean BCVA=0.6), while parameters in two patients remained unchanged. Compared to baseline (before treatment), the proportions of patients experiencing visual improvement at 2 and 3 months were 33% (n=2) and 67% (n=4), respectively. No major complications were observed during the treatment course. Conclusions Acupuncture might be a promising supplementary therapy for patients with CSC. However, this is ultimately only an exploratory study with a small sample size and no untreated comparator group to control for the natural history of the condition. Randomised controlled trials will be needed to demonstrate the efficacy and effectiveness of this approach.
Peripheral sensory neuropathy is associated with altered postocclusive reactive hyperemia in the diabetic foot
Authors: Barwick Alex L , Tessier John W , Janse de Jonge Xanne , Ivers James R , Chuter Vivienne H 
Published: July 2016

ObjectiveThis study examined whether the presence of peripheral sensory neuropathy or cardiac autonomic deficits is associated with postocclusive reactive hyperemia (reflective of microvascular function) in the diabetic foot.Research design and methods99 participants with type 2 diabetes were recruited into this cross-sectional study. The presence of peripheral sensory neuropathy was determined with standard clinical tests and cardiac autonomic function was assessed with heart rate variation testing. Postocclusive reactive hyperemia was measured with laser Doppler in the hallux. Multiple hierarchical regression was performed to examine relationships between neuropathy and the peak perfusion following occlusion and the time to reach this peak.ResultsPeripheral sensory neuropathy predicted 22% of the variance in time to peak following occlusion (p<0.05), being associated with a slower time to peak but was not associated with the magnitude of the peak. Heart rate variation was not associated with the postocclusive reactive hyperemia response.ConclusionsThis study found an association between the presence of peripheral sensory neuropathy in people with diabetes and altered microvascular reactivity in the lower limb.
Up-skilling associate clinicians in Malawi in emergency obstetric, neonatal care and clinical leadership: the ETATMBA cluster randomised controlled trial
Authors: Ellard David R , Chimwaza Wanangwa , Davies David , Simkiss Doug , Kamwendo Francis , Mhango Chisale , Quenby Siobhan , Kandala Ngianga-bakwin , O'Hare Joseph Paul 
Published: July 2016

BackgroundThe ETATMBA (Enhancing Training And Technology for Mothers and Babies in Africa) project-trained associate clinicians (ACs/clinical officers) as advanced clinical leaders in emergency obstetric and neonatal care. This trial aimed to evaluate the impact of training on obstetric health outcomes in Malawi.MethodA cluster randomised controlled trial with 14 districts of Malawi (8 intervention, 6 control) as units of randomisation. Intervention districts housed the 46 ACs who received the training programme. The primary outcome was district (health facility-based) perinatal mortality rates. Secondary outcomes included maternal mortality ratios, neonatal mortality rate, obstetric and birth variables. The study period was 2011–2013. Mortality rates/ratios were examined using an interrupted time series (ITS) to identify trends over time.ResultsThe ITS reveals an improving trend in perinatal mortality across both groups, but better in the control group (intervention, effect −3.58, SE 2.65, CI (−9.85 to 2.69), p=0.20; control, effect −17.79, SE 6.83, CI (−33.95 to −1.64), p=0.03). Maternal mortality ratios are seen to have improved in intervention districts while worsening in the control districts (intervention, effect −38.11, SE 50.30, CI (−157.06 to 80.84), p=0.47; control, effect 11.55, SE 87.72, CI (−195.87 to 218.98), p=0.90). There was a 31% drop in neonatal mortality rate in intervention districts while in control districts, the rate rises by 2%. There are no significant differences in the other secondary outcomes.ConclusionsThis is one of the first randomised studies looking at the effect of structured training on health outcomes in this setting. Notwithstanding a number of limitations, this study suggests that up-skilling this cadre is possible, and could impact positively on health outcomes.Trial registration numberISRCTN63294155; Results.
Use of extensively hydrolysed formula for refeeding neonates postnecrotising enterocolitis: a nationwide survey-based, cross-sectional study
Authors: Lapillonne Alexandre , Matar Maroun , Adleff Ariane , Chbihi Marwa , Kermorvant-Duchemin Elsa , Campeotto Florence 
Published: July 2016

ObjectiveTo evaluate the prevalence of and reasons for using extensively hydrolysed formulas (EHFs) of cow's milk proteins in the French neonatal units as well as the modality of their prescription for refeeding infants recovering from necrotising enterocolitis (NEC).MethodsA multicentre nationwide cross-sectional study using a questionnaire to address the prevalence of use and the reasons for prescribing EHF in hospitalised neonates and to examine the protocols and the actual reasons for their use for refeeding infants in recovery from NEC. The questionnaire was sent to only 1 senior neonatologist in each neonatal unit included in the study.ResultsMore than half of the French neonatal units participated in the survey. 91% of the surveyed units used EHF. Of 1969 infants hospitalised on the day the survey was run, 12% were fed on an EHF. 11% of the EHF prescriptions were due to previous NEC. The main reasons for using an EHF to feed infants post-NEC were the absence of human milk (75%) and surgical management of NEC (17%). When given, EHF was mainly prescribed for a period varying between 15 days and 3 months. None of the involved units continued using the EHF after 6 months of age. More than half of the surveyed units acknowledged hospitalising infants for the initiation of weaning EHF but only 21% of them tested these infants for cow's milk allergy.ConclusionsThe prevalence of EHF use in the French neonatal units is high. Refeeding infants post-NEC is one of the main reasons for such a high prevalence. The main incentive for using an EHF is the absence of human breast milk, either maternal or donor.
Multicountry survey of emergency and critical care medicine physicians’ fluid resuscitation practices for adult patients with early septic shock
Authors: McIntyre Lauralyn , Rowe Brian H , Walsh Timothy S , Gray Alasdair , Arabi Yaseen , Perner Anders , Gordon Anthony , Marshall John , Cook Deborah , Fox-Robichaud Alison , Bagshaw Sean M , Green Robert , Schweitzer Irwin , Turgeon Alexis , Zarychanski Ryan , English Shane , Chassé Michaël , Stiell Ian , Fergusson Dean 
Published: July 2016

ObjectivesEvidence to guide fluid resuscitation evidence in sepsis continues to evolve. We conducted a multicountry survey of emergency and critical care physicians to describe current stated practice and practice variation related to the quantity, rapidity and type of resuscitation fluid administered in early septic shock to inform the design of future septic shock fluid resuscitation trials.MethodsUsing a web-based survey tool, we invited critical care and emergency physicians in Canada, the UK, Scandinavia and Saudi Arabia to complete a self-administered electronic survey.ResultsA total of 1097 physicians’ responses were included. 1 L was the most frequent quantity of resuscitation fluid physicians indicated they would administer at a time (46.9%, n=499). Most (63.0%, n=671) stated that they would administer the fluid challenges as quickly as possible. Overall, normal saline and Ringer's solutions were the preferred crystalloid fluids used ‘often’ or ‘always’ in 53.1% (n=556) and 60.5% (n=632) of instances, respectively. However, emergency physicians indicated that they would use normal saline ‘often’ or ‘always’ in 83.9% (n=376) of instances, while critical care physicians said that they would use saline ‘often’ or ‘always’ in 27.9% (n=150) of instances. Only 1.0% (n=10) of respondents indicated that they would use hydroxyethyl starch ‘often’ or ‘always’; use of 5% (5.6% (n=59)) or 20–25% albumin (1.3% (n=14)) was also infrequent. The majority (88.4%, n=896) of respondents indicated that a large randomised controlled trial comparing 5% albumin to a crystalloid fluid in early septic shock was important to conduct.ConclusionsCritical care and emergency physicians stated that they rapidly infuse volumes of 500–1000 mL of resuscitation fluid in early septic shock. Colloid use, specifically the use of albumin, was infrequently reported. Our survey identifies the need to conduct a trial on the efficacy of albumin and crystalloids on 90-day mortality in patients with early septic shock.
Bacteraemia predictive factors among general medical inpatients: a retrospective cross-sectional survey in a Japanese university hospital
Authors: Fukui Sayato , Uehara Yuki , Fujibayashi Kazutoshi , Takahashi Osamu , Hisaoka Teruhiko , Naito Toshio 
Published: July 2016

ObjectivesThe precise criteria for obtaining blood cultures have not been established; they depend on the physician's judgement. We examined clinical parameters to determine predictive factors of bacteraemia and the need for blood cultures among general medical inpatients.DesignA retrospective cross-sectional survey.SettingA Japanese university hospital.ParticipantsAll general inpatients who had blood cultures taken from 1 January 2011 to 31 December 2012.Main MeasuresClinical information at or just before blood culture sampling was extracted from medical charts. Factors potentially predictive of bacteraemia were analysed using Fisher's exact test, followed by multivariable logistic regression model analysis.Main ResultsA total of 200 patients (male: female=119:81, 64.3±19.1 years old) comprised this study; 57 (28.5%) had positive blood culture results. Multivariable logistic regression analysis revealed that age >60 years (OR=2.75, 95% CI 1.23 to 6.48, p=0.015), female sex (OR=2.21, 95% CI 1.07 to 4.67, p=0.038), pulse rate >90 bpm (OR=5.18, 95% CI 2.25 to 12.48, p<0.001) and neutrophil percentage >80% (OR=3.61, 95% CI 1.71 to 8.00, p=0.001) were independent risk factors for positive blood culture results. The area under the receiver operating characteristic curve analysis of this model was 0.796.ConclusionsOur results emphasise the importance of taking blood cultures if the pulse rate is >90 bpm, in elderly patients and in women, and for ordering a differential white cell count.
Impact of maternal BMI and sampling strategy on the concentration of leptin, insulin, ghrelin and resistin in breast milk across a single feed: a longitudinal cohort study
Authors: Andreas Nicholas J , Hyde Matthew J , Herbert Bronwen R , Jeffries Suzan , Santhakumaran Shalini , Mandalia Sundhiya , Holmes Elaine , Modi Neena 
Published: July 2016

ObjectivesWe tested the hypothesis that there is a positive association between maternal body mass index (BMI) and the concentration of appetite-regulating hormones leptin, insulin, ghrelin and resistin in breast milk. We also aimed to describe the change in breast milk hormone concentration within each feed, and over time.SettingMothers were recruited from the postpartum ward at a university hospital in London. Breast milk samples were collected at the participants’ homes.ParticipantsWe recruited 120 healthy, primiparous, breastfeeding mothers, aged over 18 years. Mothers who smoked, had multiple births or had diabetes were excluded. Foremilk and hindmilk samples were collected from 105 women at 1 week postpartum and 92 women at 3 months postpartum.Primary and secondary outcome measuresWe recorded maternal and infant anthropometric measurements at each sample collection and measured hormone concentrations using a multiplex assay.ResultsThe concentration of leptin in foremilk correlated with maternal BMI at the time of sample collection, at 7 days (r=0.31, p=0.02) and 3 months postpartum (r=0.30, p=<0.00). Foremilk insulin correlated with maternal BMI at 3 months postpartum (r=0.22, p=0.04). Breast milk ghrelin and resistin were not correlated with maternal BMI. Ghrelin concentrations at 3 months postpartum were increased in foremilk compared with hindmilk (p=0.01). Concentrations of ghrelin were increased in hindmilk collected at 1  week postpartum compared with samples collected at 3 months postpartum (p=0.03). A trend towards decreased insulin concentrations in hindmilk was noted. Concentrations of leptin and resistin were not seen to alter over a feed.ConclusionsA positive correlation between maternal BMI and foremilk leptin concentration at both time points studied, and foremilk insulin at 3 months postpartum was observed. This may have implications for infant appetite regulation and obesity risk.
Migration and head penetration of Vitamin-E diffused cemented polyethylene cup compared to standard cemented cup in total hip arthroplasty: study protocol for a randomised, double-blind, controlled trial (E1 HIP)
Authors: Sköldenberg Olof , Rysinska Agata , Chammout Ghazi , Salemyr Mats , Muren Olle , Bodén Henrik , Eisler Thomas 
Published: July 2016

IntroductionIn vitro, Vitamin-E-diffused, highly cross-linked polyethylene (PE) has been shown to have superior wear resistance and improved mechanical properties when compared to those of standard highly cross-linked PE liners used in total hip arthroplasty (THA). The aim of the study is to evaluate the safety of a new cemented acetabular cup with Vitamin-E-doped PE regarding migration, head penetration and clinical results.Methods and analysisIn this single-centre, double-blinded, randomised controlled trial, we will include 50 patients with primary hip osteoarthritis scheduled for THA and randomise them in a 1:1 ratio to a cemented cup with either argon gas-sterilised PE (control group) or Vitamin-E-diffused PE (vitamin-e group). All patients and the assessor of the primary outcome will be blinded and the same uncemented stem will be used for all participants. The primary end point will be proximal migration of the cup at 2 years after surgery measured with radiostereometry. Secondary end points include proximal migration at other follow-ups, total migration, femoral head penetration, clinical outcome scores and hip-related complications. Patients will be followed up at 3 months and at 1, 2, 5 and 10 years postoperatively.ResultsResults will be analysed using 95% CIs for the effect size. A regression model will also be used to adjust for stratification factors.Ethics and disseminationThe ethical committee at Karolinska Institutet has approved the study. The first results from the study will be disseminated to the medical community via presentations and publications in relevant medical journals when the last patient included has been followed up for 2 years.Trial registration numberNCT02254980.
Understanding recruitment and retention in the NHS community pharmacy stop smoking service: perceptions of smoking cessation advisers
Authors: Sohanpal Ratna , Rivas Carol , Steed Liz , MacNeill Virginia , Kuan Valerie , Edwards Elizabeth , Griffiths Chris , Eldridge Sandra , Taylor Stephanie , Walton Robert 
Published: July 2016

ObjectivesTo understand views of pharmacy advisers about smoker recruitment and retention in the National Health Service community pharmacy stop smoking programme.DesignThematic framework analysis of semistructured, in-depth interviews applying the Theoretical Domains Framework and COM-B behaviour change model. We aimed to identify aspects of adviser behaviour that might be modified to increase numbers joining and completing the programme.Participants25 stop smoking advisers (13 pharmacists and 12 support staff).Setting29 community pharmacies in 3 inner east London boroughs.ResultsAdvisers had preconceived ideas about smokers likely to join or drop out and made judgements about smokers' readiness to quit. Actively recruiting smokers was accorded low priority due in part to perceived insufficient remuneration to the pharmacy and anticipated challenging interactions with smokers. Suggestions to improve smoker recruitment and retention included developing a more holistic and supportive approach using patient-centred communication. Training counter assistants were seen to be important as was flexibility to extend the programme duration to fit better with smokers’ needs.ConclusionsCessation advisers feel they lack the interpersonal skills necessary to engage well with smokers and help them to quit. Addressing advisers' behaviours about active engagement and follow-up of clients, together with regular skills training including staff not formally trained as cessation advisers, could potentially boost numbers recruited and retained in the stop smoking programme. Adjustments to the pharmacy remuneration structure to incentivise recruitment and to allow personalisation of the programme for individual smokers should also be considered.
Rationale and design of Diabetes Prevention with active Vitamin D (DPVD): a randomised, double-blind, placebo-controlled study
Authors: Kawahara Tetsuya , Suzuki Gen , Inazu Tetsuya , Mizuno Shoichi , Kasagi Fumiyoshi , Okada Yosuke , Tanaka Yoshiya 
Published: July 2016

IntroductionRecent research suggests that vitamin D deficiency may cause both bone diseases and a range of non-skeletal diseases. However, most of these data come from observational studies, and clinical trial data on the effects of vitamin D supplementation on individuals with pre-diabetes are scarce and inconsistent. The aim of the Diabetes Prevention with active Vitamin D (DPVD) study is to assess the effect of eldecalcitol, active vitamin D analogue, on the incidence of type 2 diabetes among individuals with pre-diabetes.Methods and analysisDPVD is an ongoing, prospective, multicentre, randomised, double-blind and placebo-controlled outcome study in individuals with impaired glucose tolerance. Participants, men and women aged ≥30 years, will be randomised to receive eldecalcitol or placebo. They will also be given a brief (5–10 min long) talk about appropriate calorie intake from diet and exercise at each 12-week visit. The primary end point is the cumulative incidence of type 2 diabetes. Secondary endpoint is the number of participants who achieve normoglycaemia at 48, 96 and 144 weeks. Follow-up is estimated to span 144 weeks.Ethics and disseminationAll protocols and an informed consent form comply with the Ethics Guideline for Clinical Research (Japan Ministry of Health, Labour and Welfare). The study protocol has been approved by the Institutional Review Board at Kokura Medical Association and University of Occupational and Environmental Health. The study will be implemented in line with the CONSORT statement.Trial registration numberUMIN000010758; Pre-results.