bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunophenotype of Peripheral Blood Lymphocytes in Dogs with Inflammatory Bowel Disease
Authors: Galler A. , Rütgen B.C. , Haas E. , Saalmüller A. , Hirt R.A. , Gerner W. , Schwendenwein I. , Richter B. , Thalhammer J.G. , Luckschander‐Zeller N. 
Published: September 2017

BackgroundInflammatory bowel disease (IBD) is common in dogs. Despite the known importance of intestinal lymphocytes in its pathogenesis, little is known about the role of peripheral blood lymphocytes (PBLs) inIBD.ObjectivesThe aims of this study were (1) comparison ofPBLs analyzed by flow cytometry (FCM) inIBDdogs and healthy controls and (2) comparison ofPBLs inIBDdogs at the time of diagnosis and in dogs in clinical remission.AnimalsWhole blood samples of 19IBDdogs at the time of diagnosis and blood samples of 6 dogs in clinical remission were collected. Ten healthy dogs served as controls.MethodsIn this prospective observational study,PBLs were analyzed with multicolorFCMby staining with a panel of anticanine and cross‐reactive monoclonal antibodies against T‐ and B‐cell differentiation antigens, includingCD45,CD3,CD4,CD8α,CD8β,TCRαβ,TCRγδ,CD79αcy, andCD21.ResultsTheIBDpatients’PBLs had significantly decreased percentages ofTCRγδ+T lymphocytes (median: healthy dogs, 3.32;IBDdogs, 0.97;P= 0.03) andCD21+B cells (median: healthy dogs, 27.61;IBDdogs, 17.26;P= 0.04). There were no significant differences inPBLs between pretreatment and follow‐up samples.Conclusions and Clinical ImportanceThe differences betweenPBLs in healthy andIBDdogs analyzed byFCMindicate an imbalance of lymphocytes with different immunologic functions and emphasize the potential value of this technique in a larger cohort of dogs. ThePBLs did not differ betweenIBDdogs before treatment and clinically well‐controlled dogs after treatment.
Intermineral oxygen three‐isotope systematics of silicate minerals in equilibrated ordinary chondrites
Authors: McDougal David , Nakashima Daisuke , Tenner Travis J. , Kita Noriko T. , Valley John W. , Noguchi Takaaki 
Published: September 2017

AbstractHigh‐precision oxygen three‐isotope ratios were measured for four mineral phases (olivine, low‐Ca and high‐Ca pyroxene, and plagioclase) in equilibrated ordinary chondrites (EOCs) using a secondary ion mass spectrometer. Eleven EOCs were studied that cover all groups (H, L, LL) and petrologic types (4, 5, 6), including S1–S4 shock stages, as well as unbrecciated and brecciated meteorites. SIMS analyses of multiple minerals were made in close proximity (mostly <100 μm) from several areas in each meteorite thin section, to evaluate isotope exchange among minerals. Oxygen isotope ratios in each mineral become more homogenized as petrologic type increases with the notable exception of brecciated samples. In type 4 chondrites, oxygen isotope ratios of olivine and low‐Ca pyroxene are heterogeneous in both δ18O and Δ17O, showing similar systematics to those in type 3 chondrites. In type 5 and 6 chondrites, oxygen isotope ratios of the four mineral phases plot along mass‐dependent fractionation lines that are consistent with the bulk average Δ17O of each chondrite group. The δ18O of three minerals, low‐Ca and high‐Ca pyroxene and plagioclase, are consistent with equilibrium fractionation at temperatures of 700–1000 °C. In most cases the δ18O values of olivine are higher than those expected from pyroxene and plagioclase, suggesting partial retention of premetamorphic values due to slower oxygen isotope diffusion in olivine than pyroxene during thermal metamorphism in ordinary chondrite parent bodies.
The noble gas concentrations of the Martian meteorites GRV 99027 and paired NWA 7906/NWA 7907
Authors: Stephenson Peter C. , Lin Yangting , Leya Ingo 
Published: September 2017

AbstractHere we present the isotopic concentrations of He, Ne, Ar, Kr, and Xe for the three Martian meteorites, namely Grove Mountains 99027 (GRV 99027), Northwest Africa 7906 (NWA 7906), and Northwest Africa 7907 (NWA 7907). The cosmic ray exposure (CRE) age for GRV 99027 of 5.7 ± 0.4 Ma (1σ) is consistent with CRE ages for other poikilitic basaltic shergottites and suggests that all were ejected in a single event ~5.6 Ma ago. After correcting for an estimated variable sodium concentration, the CRE ages for NWA 7906 and NWA 7907 of 5.4 ± 0.4 and 4.9 ± 0.4 Ma (1σ), respectively, are in good agreement with the CRE age of ~5 Ma favored by Cartwright et al. () for NWA 7034. The data, therefore, support the conclusion that all three basaltic regolith breccias are paired. The 40Ar gas retention age for NWA 7907 of ~1.3 Ga is in accord with Cartwright et al. (). For NWA 7906, we were unable to determine a 40Ar gas retention age. The 4He gas retention ages for NWA 7906 and 7907 are in the range of 200 Ma and are much shorter than the 40Ar gas retention age of NWA 7907, indicating that about 86–88% of the radiogenic 4He has been lost. The Kr and Xe isotopic concentrations in GRV 99027 are composed almost exclusively of Martian interior (MI) gases, while for NWA 7906 and NWA 7907, they indicate gases from the MI, elementally fractionated air, and possibly Martian atmosphere.
Complete Genome Sequence of Salmonella enterica Lytic Bacteriophage LPST10, Isolated in China
Authors: Dong Xingxing , Huang Chenxi , Morsy Mohamed Khairy , Li Zhi , Zhou Yang , Willias Stephan P. , Abdelnabby Hazem Elewa , Liu Jie , Feng Liping , Wang Xiaohong , Li Jinquan 
Published: September 2017

ABSTRACT

Bacteriophage LPST10 was isolated from Wuhan, China. Lytic activity was demonstrated against multiple
Salmonella enterica
serovars, including
Salmonella enterica
serovar Typhimurium strains. This bacteriophage has a 47,657-bp double-stranded DNA genome encoding 87 putative coding sequences.
Whole-Genome Sequence of the 1,4-Dioxane-Degrading Bacterium
Mycobacterium dioxanotrophicus
PH-06
Authors: He Ya , Wei Kangfei , Si Kaiwei , Mathieu Jacques , Li Mengyan , Alvarez Pedro J. J. 
Published: September 2017

ABSTRACT

We report here the complete genome sequence of
Mycobacterium dioxanotrophicus
PH-06, which is capable of using 1,4-dioxane as a sole source of carbon and energy. The reported sequence will enable the elucidation of this novel metabolic pathway and the development of molecular biomarkers to assess bioremediation potential at contaminated sites.
Draft Genome Sequence of Photorhabdus luminescens HIM3 Isolated from an Entomopathogenic Nematode in Agricultural Soils
Authors: Salgado-Morales Rosalba , Rivera-Gómez Nancy , Martínez-Ocampo Fernando , Lozano-Aguirre Beltrán Luis Fernando , Hernández-Mendoza Armando , Dantán-González Edgar 
Published: September 2017

ABSTRACT

In this work, we report the draft genome sequence of
Photorhabdus luminescens
strain HIM3, a symbiotic bacterium associated with the entomopathogenic nematode
Heterorhabditis indica
MOR03, isolated from soil sugarcane in Yautepec, Morelos, Mexico. These bacteria have a G+C content of 42.6% and genome size of 5.47 Mb.
Whole-Genome Sequence of Streptococcus tigurinus Strain osk_001, Isolated from Postmortem Material
Authors: Yoshizawa Hidenori , Motooka Daisuke , Katada Ryuichi , Matsumoto Yuki , Nakamura Shota , Morii Eiichi , Iida Tetsuya , Matsumoto Hiroshi 
Published: September 2017

ABSTRACT

Streptococcus tigurinus
was recently described as a novel species, and some strains are highly virulent. We detected
S. tigurinus
in infected tissue sampled by necropsy. In order to characterize and confirm the virulence of this species, whole-genome sequencing of the pure cultured bacterium was performed. We found that the strain has specific and unique genetic elements contained in highly virulent strains of
S. tigurinus
.
Whole-Genome Sequences of Brucella melitensis Strain QY1, Isolated from Sheep in Gansu, China
Authors: Cao Xiaoan , Li Zhaocai , Lou Zhongzi , Fu Baoquan , Liu Yongsheng , Shang Youjun , Jing Zhizhong , Zhou Jizhang 
Published: September 2017

ABSTRACT

The facultative intracellular Gram-negative bacterium
Brucella melitensis
causes brucellosis in domestic and wild mammals, and it is a dominant pathogen responsible for human disease. This study reports the whole-genome sequencing of
B. melitensis
strain QY1, isolated from sheep suffering from abortion and arthritis in 2015 in Gansu, China.
Complete Genome Sequence of
Vibrio
sp. Strain 2521-89, a Close Relative of Vibrio cholerae Isolated from Lake Water in New Mexico, USA
Authors: Liang Kevin , Orata Fabini D. , Winkjer Nathan S. , Rowe Lori A. , Tarr Cheryl L. , Boucher Yan 
Published: September 2017

ABSTRACT

Vibrio
sp. strain 2521-89 is an environmental isolate from lake water in New Mexico, USA. Average nucleotide identity,
in silico
DNA-DNA hybridization, and core genome single-nucleotide polymorphism (SNP)-based phylogenetic analysis suggest that this may be a potentially novel species that is closely related to
Vibrio cholerae
.
Early detection of perceived risk among users of a UK travel health website compared with internet search activity and media coverage during the 2015–2016 Zika virus outbreak: an observational study
Authors: Petersen Jakob , Simons Hilary , Patel Dipti , Freedman Joanne 
Published: September 2017

ObjectivesThe Zika virus (ZIKV) outbreak in the Americas in 2015–2016 posed a novel global threat due to the association with congenital malformations and its rapid spread. Timely information about the spread of the disease was paramount to public health bodies issuing travel advisories. This paper looks at the online interaction with a national travel health website during the outbreak and compares this to trends in internet searches and news media output.MethodsTime trends were created for weekly views of ZIKV-related pages on a UK travel health website, relative search volumes for ‘Zika’ on Google UK, ZIKV-related items aggregated by Google UK News and rank of ZIKV travel advisories among all other pages between 15 November 2015 and 20 August 2016.ResultsTime trends in traffic to the travel health website corresponded with Google searches, but less so with media items due to intense coverage of the Rio Olympics. Travel advisories for pregnant women were issued from 7 December 2015 and began to increase in popularity (rank) from early January 2016, weeks before a surge in interest as measured by Google searches/news items at the end of January 2016.ConclusionsThe study showed an amplification of perceived risk among users of a national travel health website weeks before the initial surge in public interest. This suggests a potential value for tools to detect changes in online information seeking behaviours for predicting periods of high demand where the routine capability of travel health services could be exceeded.
Anterior transversalis fascia approach versus preperitoneal space approach for inguinal hernia repair in residents in northern China: study protocol for a prospective, multicentre, randomised, controlled trial
Authors: Fan Qing , Zhang De-wei , Yang Da-ye , Li Hong-wu , Wei Shi-bo , Yang Liang , Yang Fu-quan , Zhang Shao-jun , Wu Yao-qiang , An Wei-de , Dai Zhong-shu , Jiang Hui-yong , Wang Fu-rong , Qiao Shi-feng , Li Hang-yu 
Published: September 2017

IntroductionMany surgical techniques have been used to repair abdominal wall defects in the inguinal region based on the anatomic characteristics of this region and can be categorised as ‘tension’ repair or ‘tension-free’ repair. Tension-free repair is the preferred technique for inguinal hernia repair. Tension-free repair of inguinal hernia can be performed through either the anterior transversalis fascia approach or the preperitoneal space approach. There are few large sample, randomised controlled trials investigating the curative effects of the anterior transversalis fascia approach versus the preperitoneal space approach for inguinal hernia repair in patients in northern China.Methods and analysisThis will be a prospective, large sample, multicentre, randomised, controlled trial. Registration date is 1 December 2016. Actual study start date is 6 February 2017. Estimated study completion date is June 2020. A cohort of over 720 patients with inguinal hernias will be recruited from nine institutions in Liaoning Province, China. Patient randomisation will be stratified by centre to undergo inguinal hernia repair via the anterior transversalis fascia approach or the preperitoneal approach. Primary and secondary outcome assessments will be performed at baseline (prior to surgery), predischarge and at postoperative 1 week, 1 month, 3 months, 1 year and 2 years. The primary outcome is the incidence of postoperative chronic inguinal pain. The secondary outcome is postoperative complications (including rates of wound infection, haematoma, seroma and hernia recurrence).Ethics and disseminationThis trial will be conducted in accordance with the Declaration of Helsinki and supervised by the institutional review board of the Fourth Affiliated Hospital of China Medical University (approval number 2015–027). All patients will receive information about the trial in verbal and written forms and will give informed consent before enrolment. The results will be published in peer-reviewed journals or disseminated through conference presentations.Trial registration numberNCT02984917; preresults.
Risk factors for falls in older patients with cancer
Authors: Zhang Xiaotao , Sun Ming , Liu Suyu , Leung Cheuk Hong , Pang Linda , Popat Uday R , Champlin Richard , Holmes Holly M , Valero Vicente , Dinney Colin P , Tripathy Debu , Edwards Beatrice J 
Published: September 2017

ObjectivesA rising number of patients with cancer are older adults (65 years of age and older), and this proportion will increase to 70% by the year 2020. Falls are a common condition in older adults. We sought to assess the prevalence and risk factors for falls in older patients with cancer.MethodsThis is a single-site, retrospective cohort study. Patients who were receiving cancer care underwent a comprehensive geriatric assessments, including cognitive, functional, nutritional, physical, falls in the prior 6 months and comorbidity assessment. Vitamin D and bone densitometry were performed.AnalysisDescriptive statistics and multivariable logistic regression.ResultsA total of 304 patients aged 65 or above were enrolled in this study. The mean age was 78.4±6.9 years. They had haematological, gastrointestinal, urological, breast, lung and gynaecological cancers. A total of 215 patients with available information about falls within the past 6 months were included for final analysis. Seventy-seven (35.8%) patients had at least one fall in the preceding 6 months. Functional impairment (p=0.048), frailty (p<0.001), dementia (p=0.021), major depression (p=0.010) and low social support (p=0.045) were significantly associated with the fall status in the univariate analysis. Multivariate logistic regression analysis identified frailty and functional impairment to be independent risk factors for falls.ConclusionsFalls are common in older patients with cancer and lead to adverse clinical outcomes. Major depression, functional impairment, frailty, dementia and low social support were risk factors for falls. Heightened awareness and targeted interventions can prevent falls in older patients with cancer.
Assessment of Sepsis-3 criteria and quick SOFA in patients with cirrhosis and bacterial infections
Authors: Piano Salvatore , Bartoletti Michele , Tonon Marta , Baldassarre Maurizio , Chies Giada , Romano Antonietta , Viale Pierluigi , Vettore Elia , Domenicali Marco , Stanco Marialuisa , Pilutti Chiara , Frigo Anna Chiara , Brocca Alessandra , Bernardi Mauro , Caraceni Paolo , Angeli Paolo 
Published: September 2017

Introduction
Patients with cirrhosis have a high risk of sepsis, which confers a poor prognosis. The systemic inflammatory response syndrome (SIRS) criteria have several limitations in cirrhosis. Recently, new criteria for sepsis (Sepsis-3) have been suggested in the general population (increase of Sequential Organ Failure Assessment (SOFA) ≥2 points from baseline). Outside the intensive care unit (ICU), the quick SOFA (qSOFA (at least two among alteration in mental status, systolic blood pressure ≤100 mm Hg or respiratory rate ≥22/min)) was suggested to screen for sepsis. These criteria have never been evaluated in patients with cirrhosis. The aim of the study was to assess the ability of Sepsis-3 criteria in predicting in-hospital mortality in patients with cirrhosis and bacterial/fungal infections.

Methods
259 consecutive patients with cirrhosis and bacterial/fungal infections were prospectively included. Demographic, laboratory and microbiological data were collected at diagnosis of infection. Baseline SOFA was assessed using preadmission data. Patients were followed up until death, liver transplantation or discharge. Findings were externally validated (197 patients).

Results
Sepsis-3 and qSOFA had significantly greater discrimination for in-hospital mortality (area under the receiver operating characteristic (AUROC)=0.784 and 0.732, respectively) than SIRS (AUROC=0.606) (p<0.01 for both). Similar results were observed in the validation cohort. Sepsis-3 (subdistribution HR (sHR)=5.47; p=0.006), qSOFA (sHR=1.99; p=0.020), Chronic Liver Failure Consortium Acute Decompensation score (sHR=1.05; p=0.001) and C reactive protein (sHR=1.01;p=0.034) were found to be independent predictors of in-hospital mortality. Patients with Sepsis-3 had higher incidence of acute-on-chronic liver failure, septic shock and transfer to ICU than those without Sepsis-3.

Conclusions
Sepsis-3 criteria are more accurate than SIRS criteria in predicting the severity of infections in patients with cirrhosis. qSOFA is a useful bedside tool to assess risk for worse outcomes in these patients. Patients with Sepsis-3 and positive qSOFA deserve more intensive management and strict surveillance.
Small animal disease surveillance: GI disease and salmonellosis
Authors: Arsevska Elena , Singleton David , Sánchez‐Vizcaíno Fernando , Williams Nicola , Jones Philip H. , Smyth Steven , Heayns Bethaney , Wardeh Maya , Radford Alan D. , Dawson Susan , Noble Peter J. M. , Davies Robert H. 
Published: September 2017

Presentation for gastrointestinal (GI) disease comprised 2.2 per cent of cat, 3.2 per cent of dog and 2.2 per cent of rabbit consultations between April 1, 2016 and March 31, 2017
Diarrhoea and vomiting without blood were the most frequently reported GI disease clinical signs (34.4 and 38.9 per cent in cats and 42.8 and 37.3 per cent in dogs, respectively)
The mean percentage of samples testing positive for Salmonella in dogs was double that in cats (0.82 per cent and 0.41 per cent, respectively) from January 1, 2011 to December 31, 2016
In dogs, autumn was associated with a greater proportion of Salmonella‐positive sample submissions; no clear suggestion of seasonal variation in cats was observed
In both cats and dogs, isolates belonging to Salmonella enterica group B serotypes were the most common (68.9 per cent in cats and 55.0 per cent in dogs)
Measuring high-altitude adaptation
Authors: Moore Lorna G. 
Published: September 2017

High altitudes (>8,000 ft or 2,500 m) provide an experiment of nature for measuring adaptation and the physiological processes involved. Studies conducted over the past ~25 years in Andeans, Tibetans, and, less often, Ethiopians show varied but distinct O2transport traits from those of acclimatized newcomers, providing indirect evidence for genetic adaptation to high altitude. Short-term (acclimatization, developmental) and long-term (genetic) responses to high altitude exhibit a temporal gradient such that, although all influence O2content, the latter also improve O2delivery and metabolism. Much has been learned concerning the underlying physiological processes, but additional studies are needed on the regulation of blood flow and O2utilization. Direct evidence of genetic adaptation comes from single-nucleotide polymorphism (SNP)-based genome scans and whole genome sequencing studies that have identified gene regions acted upon by natural selection. Efforts have begun to understand the connections between the two with Andean studies on the genetic factors raising uterine blood flow, fetal growth, and susceptibility to Chronic Mountain Sickness and Tibetan studies on genes serving to lower hemoglobin and pulmonary arterial pressure. Critical for future studies will be the selection of phenotypes with demonstrable effects on reproductive success, the calculation of actual fitness costs, and greater inclusion of women among the subjects being studied. The well-characterized nature of the O2transport system, the presence of multiple long-resident populations, and relevance for understanding hypoxic disorders in all persons underscore the importance of understanding how evolutionary adaptation to high altitude has occurred.NEW & NOTEWORTHY Variation in O2transport characteristics among Andean, Tibetan, and, when available, Ethiopian high-altitude residents supports the existence of genetic adaptations that improve the distribution of blood flow to vital organs and the efficiency of O2utilization. Genome scans and whole genome sequencing studies implicate a broad range of gene regions. Future studies are needed using phenotypes of clear relevance for reproductive success for determining the mechanisms by which naturally selected genes are acting.
Additive effects of heating and exercise on baroreflex control of heart rate in healthy males
Authors: Peçanha Tiago , Forjaz Cláudia L. M. , Low David. A. 
Published: September 2017

This study assessed the additive effects of passive heating and exercise on cardiac baroreflex sensitivity (cBRS) and heart rate variability (HRV). Twelve healthy young men (25 ± 1 yr, 23.8 ± 0.5 kg/m2) randomly underwent two experimental sessions: heat stress (HS; whole body heat stress using a tube-lined suit to increase core temperature by ~1°C) and normothermia (NT). Each session was composed of a preintervention rest (REST1); HS or NT interventions; postintervention rest (REST2); and 14 min of cycling exercise [7 min at 40%HRreserve(EX1) and 7 min at 60%HRreserve(EX2)]. Heart rate and finger blood pressure were continuously recorded. cBRS was assessed using the sequence (cBRSSEQ) and transfer function (cBRSTF) methods. HRV was assessed using the indexes standard deviation of RR intervals (SDNN) and root mean square of successive RR intervals (RMSSD). cBRS and HRV were not different between sessions during EX1 and EX2 (i.e., matched heart rate conditions: EX1 = 116 ± 3 vs. 114 ± 3 and EX2 = 143 ± 4 vs. 142 ± 3 beats/min but different workloads: EX1 = 50 ± 9 vs. 114 ± 8 and EX2 = 106 ± 10 vs. 165 ± 8 W; for HS and NT, respectively; P < 0.01). However, when comparing EX1 of NT with EX2 of HS (i.e., matched workload conditions but with different heart rates), cBRS and HRV were significantly reduced in HS (cBRSSEQ = 1.6 ± 0.3 vs. 0.6 ± 0.1 ms/mmHg, P < 0.01; SDNN = 2.3 ± 0.1 vs. 1.3 ± 0.2 ms, P < 0.01). In conclusion, in conditions matched by HR, the addition of heat stress to exercise does not affect cBRS and HRV. Alternatively, in workload-matched conditions, the addition of heat to exercise results in reduced cBRS and HRV compared with exercise in normothermia.NEW & NOTEWORTHY The present study assessed cardiac baroreflex sensitivity during the combination of heat and exercise stresses. This is the first study to show that prior whole body passive heating reduces cardiac baroreflex sensitivity and autonomic modulation of heart rate during exercise. These findings contribute to the better understanding of the role of thermoregulation on cardiovascular regulation during exercise.