bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heme Biosynthetic Pathway is Functionally Linked to Adipogenesis via Mitochondrial Respiratory Activity
Authors: Moreno‐Navarrete José María , Rodríguez Amaia , Ortega Francisco , Becerril Sara , Girones Jordi , Sabater‐Masdeu Mònica , Latorre Jéssica , Ricart Wifredo , Frühbeck Gema , Fernández‐Real José Manuel 
Published: August 2017

ObjectiveTo investigate key enzymes of heme biosynthesis in human adipocytes and adipose tissue (AT).MethodsHeme biosynthesis‐related gene expression (ALAS1, ALAD, HMBS) was investigated in whole AT from humans (n = 178 and n = 75) and rats according to obesity status and during adipogenesis of human preadipocytes. The effects of aminotriazole (an ALAD inhibitor) and of ALAD knockdown were also studied.ResultsConsistent heme biosynthesis‐related gene expression was detected in both subcutaneous AT (SAT) and visceral AT (VAT) and was significantly increased in SAT. ALAS1, ALAD, and HMBS mRNAs were positively associated with adipogenic gene expression in human AT and significantly decreased in subjects with obesity. These results were replicated in an independent cohort. Both SAT and VAT heme levels were positively correlated with ALAS1, ALAD, and HMBS mRNAs. ALAD and HMBS were mainly expressed in adipocytes and increased during differentiation of human adipocytes in parallel to adipogenic genes. In rats, high‐fat diet‐induced weight gain resulted in decreased Alad and Hmbs mRNAs in a similar way to what was observed with Adipoq. Aminotriazole administration or ALAD knockdown attenuated adipogenesis in parallel with decreased glucose uptake and impaired mitochondrial respiratory function during human adipocyte differentiation.ConclusionsCurrent data suggest a possible role of heme biosynthesis in human adipogenesis.
Complexation of bis‐crown stilbene with alkali and alkaline‐earth metal cations. Ultrafast excited state dynamics of the stilbene‐viologen analogue charge transfer complex
Authors: Volchkov Valery V. , Rusalov Mikhail V. , Gostev Fedor E. , Shelaev Ivan V. , Nadtochenko Viktor A. , Vedernikov Artem I. , Efremova Asya A. , Kuz'mina Lyudmila G. , Gromov Sergey P. , Alfimov Michael V. , Ya. Melnikov Mikhail 
Published: August 2017

AbstractThe complex formation of bis(18‐crown‐6)stilbene (1) and its supramolecular donor‐acceptor complex with N,N′‐bis(ammonioethyl) 1,2‐di(4‐pyridyl)ethylene derivative (2) with alkali and alkaline‐earth metal perchlorates has been studied using absorption, steady‐state fluorescence, and femtosecond transient absorption spectroscopy. The formation of 1∙Mn+ and 1∙(Mn+)2 complexes in acetonitrile was demonstrated. The weak long‐wavelength charge‐transfer absorption band of 1·2 completely vanishes upon complexation with metal cations because of disruption of the pseudocyclic structure. The spectroscopic and luminescence parameters, stability constants, and 2‐stage dissociation constants were calculated. The initial stage of a recoordination process was found in the excited complexes 1∙M+ and 1∙(M+)2 (M = Li, Na). The pronounced fluorescence quenching of 1·2 is explained by very fast back electron transfer (τet = 0.397 ps). The structure of complex 1·2 was studied by X‐ray diffraction; stacked (1·2)m polymer in which the components were connected by hydrogen bonding and stacking was found in the crystal. These compounds can be considered as novel optical molecular sensors for alkali and alkaline‐earth metal cations.
Rigid diol bearing 6‐6‐6 fused ring system derived from naturally occurring myo‐inositol and its polyaddition with diisocyanates
Authors: Yoshida Arata , Sudo Atsushi 
Published: August 2017

ABSTRACTNaturally occurring myo‐inositol was developed into a highly rigid diol by converting its 3,4‐ and 1,6‐vicinal diols in trans configuration into the corresponding butane‐2,3‐diacetals. The resulting diol bearing 6‐6‐6 fused ring system, in which conformational change is strictly suppressed, was combined with diisocyanates to perform polyadditions. The resulting polyurethanes were analyzed by differential scanning calorimetry, and it was found that their glass transition temperatures were much higher than those of the previously reported myo‐inositol‐derived polyurethanes, which were synthesized from a myo‐inositol‐derived diol bearing 5‐6‐5 fused ring system. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 3798–3803
Effect of Sandpaper and Grain Size on Non‐Shock Initiated Reactions in HMX
Authors: Reynolds John G. , Hsu Peter C. , Hust Gary A. , Strout Stephen A. , Hoffman D. Mark , Springer H. Keo 
Published: August 2017

AbstractThree grades of HMX samples, coarse (A), coarse with a small amount of fines (B), and ultra‐fine (F), were tested for impact sensitivity by ERL drop hammer. The Type 12A test used sandpapers of three different compositions, 120‐, 180‐grit Si/C and 180‐grit garnet. Reaction was accounted for by operator observation and microphone. The results showed different sensitivity for each of the HMX types. The F‐HMX was the smallest in average particle size and was the least sensitive to impact. The A‐HMX was the next most stable and the B‐HMX was the most sensitive to impact. The spent samples from the drop hammer testing of B‐HMX and F‐HMX were further evaluated with optical imaging. Conditions were selected that were near the DH50 values of the specific HMX. Previous literature has cited localized hot spot formation to be the probable cause of non‐shock initiated reactions leading to impact sensitivity. This study yielded a plethora of samples exhibiting hot spots for HMX materials, both without and with proximity to grit particles. Hot spots in the proximity of grit particles gave the most dramatic hot spots, but both types of hot spots were exhibited in samples considered reacted and non‐reacted. The F‐HMX, even though the most stable to impact sensitivity, exhibited the most and best resolved hot spots of all the samples and conditions. Foreign objects were also observed in some of the samples. Previous work has shown metal particles coming from wear of the anvils used in the drop hammer experiment can also form hot spots. However, none of the samples here with metal particles exhibited, in proximity, hot spot formation.
Capturing the photo‐signaling state of a photoreceptor in a steady‐state fashion by binding a transition metal complex
Authors: Yu Pengyun , Song Lei , Qin Jun , Wang Jianping 
Published: August 2017

AbstractBinding a small molecule to proteins causes conformational changes, but often to a limited extent. Here, we demonstrate that the interaction of a CO‐releasing molecule (CORM3) with a photoreceptor photoactive yellow protein (PYP) drives large structural changes in the latter. The interaction of CORM3 and a mutant of PYP, Met100Ala, not only trigger the isomerization of its chromophore, p‐coumaric acid, from its anionic trans configuration to a protonated cis configuration, but also increases the content of β‐sheet at the cost of α‐helix and random coil in the secondary structure of the protein. The CORM3 derived Met100Ala is found to highly resemble the signaling state, which is one of the key photo‐intermediates of this photoactive protein, in both protein local conformation and chromophore configuration. The organometallic reagents hold promise as protein engineering tools. This work highlights a novel approach to structurally accessing short lived intermediates of proteins in a steady‐state fashion.
Identification of heteromolecular binding sites in transcription factors Sp1 and TAF4 using high‐resolution nuclear magnetic resonance spectroscopy
Authors: Hibino Emi , Inoue Rintaro , Sugiyama Masaaki , Kuwahara Jun , Matsuzaki Katsumi , Hoshino Masaru 
Published: August 2017

AbstractThe expression of eukaryotic genes is precisely controlled by interactions between general transcriptional factors and promoter‐specific transcriptional activators. The fourth element of TATA‐box binding protein‐associated factor (TAF4), an essential subunit of the general transcription factor TFIID, serves as a coactivator for various promoter‐specific transcriptional regulators. Interactions between TAF4 and site‐specific transcriptional activators, such as Sp1, are important for regulating the expression levels of genes of interest. However, only limited information is available on the molecular mechanisms underlying the interactions between these transcriptional regulatory proteins. We herein analyzed the interaction between the transcriptional factors Sp1 and TAF4 using high‐resolution solution nuclear magnetic resonance spectroscopy. We found that four glutamine‐rich (Q‐rich) regions in TAF4 were largely disordered under nearly physiological conditions. Among them, the first Q‐rich region in TAF4 was essential for the interaction with another Q‐rich region in the Sp1 molecule, most of which was largely disordered. The residues responsible for this interaction were specific and highly localized in a defined region within a range of 20–30 residues. Nevertheless, a detailed analysis of 13C‐chemical shift values suggested that no significant conformational change occurred upon binding. These results indicate a prominent and exceptional binding mode for intrinsically disordered proteins other than the well‐accepted concept of “coupled folding and binding.”
Monitoring simple linear profiles in the presence of generalized autoregressive conditional heteroscedasticity
Authors: Hadizadeh Reza , Soleimani Paria 
Published: August 2017

AbstractIn this paper, monitoring of simple linear profiles is investigated in the presence of nonequality of variances or heteroscedasticity, ie, generalized autoregressive conditional heteroscedasticity. In this condition, using of the common methods regardless of the heteroscedasticity leads to the fault interpretations. We consider a simple linear profile and assume that there is a generalized autoregressive conditional heteroscedasticity (GARCH) (1,1) model within the profiles. Here, we particularly focus on Phase II monitoring of simple linear regression. We studied the generalized autoregressive conditional heteroscedasticity effect, briefly GARCH effect, on the average run length criterion. As the remedial measures, the weighted least squares method to estimate the regression parameters and the heteroscedasticity‐consistent approaches to estimate the covariance matrix of regression parameters, are used to extract the GARCH effect. Two control chart methods namely T2 and exponentially weighted moving average 3 are discussed to monitor the simple linear profiles. Their performances are evaluated by using the average run length criterion. Finally, a real case from an industry field is studied.
Ion suppression effect in desorption electrospray ionization and electrospray ionization mass spectrometry
Authors: Wang Zheng , Zhu Haijing , Huang Guangming 
Published: August 2017

RationaleAlthough it is claimed that desorption electrospray ionization (DESI) causes less ion suppression effects than electrospray ionization (ESI), a related investigation with quantification measurement of ion suppression effects is absent. Herein, a comparative analysis of ion suppression effects between DESI and ESI was conducted, with the aiming of quantitatively studying the ion suppression effect.MethodsCTVA mixtures, a constant concentration of tioconazole with varied concentrations of atenolol, were analyzed by ESI and DESI. The ion suppression effect was characterized by the signal loss of tioconazole in the mixture compared to the signal intensity of tioconazole without interference (denoted as (Isingle − Imixture)/Isingle). According to the variations in the experimental conditions (such as flow rate, solvent composition, substrate material, capillary inner diameter, sheath gas velocity), ion suppression effects in DESI and ESI were compared.ResultsWith the increasing flow rate, the ion suppression effect in DESI became weaker, while the opposite trend was obtained for ESI. As for capillary inner diameter, a smaller inner diameter resulted in weaker ion suppression effects in DESI and ESI. The solvent composition affected the ion suppression effect, and the PTFE substrate presented the weakest ion suppression effect among the five substrate materials.ConclusionsThough the ion suppression effect in DESI and ESI was shown to relate to experimental conditions, DESI had less effect than ESI under the same experimental conditions in most cases. Moreover, DESI displayed stronger matrix‐tolerant ability than ESI which is also attributed to the weaker ion suppression effect.
Electrochemical Detection of Ni2+ Ions Using Synthesized (E)‐N’‐Chlorobenzylidene‐4‐methylbenzenesulfonohydrazide Derivatives Modified with a Nafion Matrix
Authors: Hussain Mohammad Musarraf , Rahman Mohammed M. , Arshad Muhammad Nadeem , Asiri Abdullah M. 
Published: August 2017

Abstract(E)‐N’‐Chlorobenzylidene‐4‐methylbenzenesulfonohydrazide (CBMBSH) compounds were synthesized using a simple condensation process from the derivatives of chlorobenzaldehyde and 4‐methylbenzenesulfonylhydrazine in a good yield which crystallized in ethanol. The CBMBSH molecules were totally characterized using conventional spectroscopic techniques such as 1H‐NMR, 13C‐NMR, FTIR, and UV‐Vis spectroscopy. The molecular structures of the CBMBSH molecules were confirmed using a single crystal X‐ray diffraction (SCXRD) method and used for the potential detection of heavy metal ion, nickel (Ni2+) using a reliable I−V technique. A thin layer of CBMBSH was deposited onto a flat glassy carbon electrode (GCE) with 5 % nafion coating binder in order to modify a sensitive and selective Ni2+ ionic sensor. Sensitivity, limit of detection (LOD), and limit of quantification (LOQ) of the modified electrode towards Ni2+ were calculated from the slope of calibration curve as 949.0 pAμM−1cm−2, 98.0±2.0 pM, and 326.67 mM respectively. The approaching CBMBSH/GCE sensor was applied to the selective determination of Ni2+ in spiked real samples, and found satisfactory and rational results.
Gallium‐Induced Perturbation of Zinc Selenide Quantum Dots Electronics
Authors: Ndangili Peter M. , Masikini Milua , Feleni Usisipho , Douman Samantha , Tovide Oluwakemi , Williams Avril , Baker Priscilla , Iwuoha Emmanuel I. 
Published: August 2017

AbstractA rare cationic chemistry of GaIII has been used to modulate the optical and electrochemical properties of selenide quantum dots. Three different types of 3‐mercaptopropionic acid (3MPA)‐capped quantum dots (ZnSe‐3MPA, Ga2Se3‐3MPA and Ga‐doped ZnSe‐3MPA) were synthesized in highly basic aqueous media (pH = 12.12) at room temperature. Three‐dimensional emission‐excitation matrix spectra (3D EEM), as well as, the ultraviolet visible spectroscopic bands of the Ga‐doped ZnSe‐3MPA were similar to the average values obtained for ZnSe‐3MPA and Ga2Se3‐3MPA. Electrochemical studies revealed that gallium‐induced vacancies caused a significant enhancement in the conductivity of the Ga‐doped ZnSe‐3MPA compared to the conductivity of a mixture of ZnSe‐3MPA and Ga2Se3‐3MPA.
Highly Sensitive Surface‐Enhanced Raman Scattering (SERS)‐ Based Multi Gas Sensor : Au Nanoparticles Decorated on Partially Embedded 2D Colloidal Crystals into Elastomer
Authors: Sharma Satinder K. , Kumar Pawan , Barthwal Sumit , Sharma Seema , Sharma Ashutosh 
Published: August 2017

AbstractAdvent of surface‐enhanced Raman spectroscopy (SERS) has engendered, the attention to exploit a facile, cost effective, 2D self‐assembly based architecture for sensing. Here, Au nanoparticles are decorated on controlled morphology of 2D polystyrene colloidal crystals (PSCCs), partially embedded into flexible polydimethylsiloxane elastomer. Self‐assembled flexible hexagonally close‐packed PSCCs form the 75 and 25 nm voids in the 500 and 200 nm, PSCCs monolayers and act as an effective plasmonic propagator for SERS sensors signal. Optical and sensing response investigates with UV‐Vis and Raman spectroscopy after the exposure of Au NP/2D PS CC/PDMS to NH3, H2O2, N2O and H2S gases environment. The increase in the SERS intensity at 491 cm−1 for the 200 nm, CCs based sensors as compared to 500 nm, indicates that the sensing response, strongly dependent on the CCs size. The red‐shift in the UV‐Vis spectra and enhancement in intensity of SERS designate that H2S forms the maximum Au NPs aggregates over the all tested gases. The chemisorptions on the phenyl ring, due to B2 symmetric modes of the PSCCs with orthogonal polarizability of Au NPs, act as a hot spot for higher sensing response for the detection of gaseous precursors with higher SERS sensitivity and reliability.
Enhancing the Activity of a Dietzia sp. D5 Baeyer‐Villiger Monooxygenase towards Cyclohexanone by Saturation Mutagenesis
Authors: Bisagni Serena , Abolhalaj Milad , de Brevern Alexandre G. , Rebehmed Joseph , Hatti‐Kaul Rajni , Mamo Gashaw 
Published: August 2017

AbstractA recombinant Baeyer‐Villiger monooxygenase, BVMO4 from Dietzia sp. D5 has been previously reported. The enzyme exhibited good thermostability and was active with a wide range of cyclic ketone substrates but catalysed poorly the conversion of cyclohexanone to caprolactone. The present work focuses on protein engineering of BVMO4 to improve the conversion of cyclohexanone. Thus, a structural model of BVMO4 was generated and used in combination with literature information on substrate specificity of BVMOs to identify ‘hotspots’ whose mutation would influence substrate specificity. Site saturation mutagenesis was performed on 12 selected sites and 528 mutants were screened with expected coverage of about 98 %. About one‐fourth of the mutants screened exhibited more than 50 % increase in cyclohexanone oxidation activity. Compared to the wild type BVMO, the best mutants, Y499I, Y499F and Y499 L have shown about 12‐fold increase for caprolactone production.
Synthesis of Novel 5‐Substituted 6‐Phenylpyrrolo[2, 3‐d]pyrimidine Derivatives via One‐pot Three‐Component Reactions Under Catalyst‐Free Condition
Authors: Saroha Mohit , Khanna Garima , Khurana Jitender M. 
Published: August 2017

AbstractAn efficient synthesis of some novel 5‐subsituted 6‐phenyl pyrrolo[2, 3‐d]pyrimidine derivatives has been reported. The 5‐substitution has been reported with indoles, 1H‐pyrazol‐5(4H)‐one, and various heterocyclic/carbocyclic active methylene compounds. The synthesis has been achieved by one‐pot three‐component condensation of phenylglyoxal, 6‐amino‐1,3‐dimethyluracil and substituted indoles/1H‐pyrazol‐5(4H)‐one/ active methylene compounds in ethanol at reflux temperature under catalyst‐free condition.
Bio‐Inspired Rose‐Like Bi@Nitrogen‐Enriched Carbon towards High‐Performance Lithium‐Ion Batteries
Authors: Lan Jin‐Le , Jin Yuqiang , Qin Chengjie , Yu Yunhua , Yang Xiaoping 
Published: August 2017

AbstractRose‐like Bi@N‐doped carbon nanocomposite was fabricate by a facile hydrothermal method, followed by polydopamine coating for different time and subsequent carbonization for the first time. Structural and morphological characterization shows that Bi@N‐doped carbon nanocomposite have a homogeneous rose‐like structure, with crystallized Bi nanoparticles embedded homogeneously in the carbon matrix. The optimal Bi@N‐doped carbon nanocomposite used as anode for rechargeable lithium‐ion batteries (LIBs) presents a reversible capacity of 712 mAh g−1 for initial cycle at a current density of 200 mA g−1 and exhibits outstanding cyclability with a capacity of 535 mAh g−1 after 450 cycles at 200 mA g−1. The outstanding electrochemical performance of the Bi@N‐doped carbon nanocomposite can be ascribed to the synergetic effects of various factors including the unique rose‐like structure, nano‐sized Bi particles and nitrogen doping carbon matrices.
Phosphorylated κ‐Carrageenan‐Facilitated Chitosan Nanovehicle for Sustainable Anti‐Tuberculosis Multi Drug Delivery
Authors: Amarnath Praphakar Rajendran , Alarfaj Abdulla A. , Munusamy Murugan A. , Dusthackeer Vijayan N. Azger , Kumar Subbiah Suresh , Rajan Mariappan 
Published: August 2017

AbstractPhosphorylated κ‐carrageenan has the potential to exhibit higher antioxidant activity than other polysaccharides. Low‐molecular‐weight κ‐carrageenan polysaccharides (LMW κ‐CAR) were prepared through oxidative degradation of high‐molecular‐weight κ‐carrageenan, and LMW κ‐CAR subjected into phosphorylation. Phosphorylated κ‐carrageenan (PCAR)‐chitosan (CS) nanoparticles was prepared by emulsification technique, followed by ionic gelation technique. Rifampicin (RF) and isoniazid (INH) were used as model drugs and encapsulated in CS‐PCAR nanocarriers. The encapsulation of drugs (RF and INH) in the CS‐PCAR polymeric carrier was verified by Fourier transform infrared spectroscopy (FT‐IR). The analysis of X‐ray diffraction (XRD) data demonstrated both RF and INH were loaded in the CS‐PCAR nanocarrier in an amorphous form. As observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), CS‐PCAR nanoparticles shows spherical structure with soft surface and exhibited good aggregation, whereas INH and RF loaded CS‐PCAR nanoparticles had varying shapes like hexagonal, that indicates their different compositions and structures. Encapsulation efficiency was determined by UV‐Vis, which was interrelated to the initial concentration of INH and RF. Cumulative release of RF and INH from CS‐PCAR nanoparticles was observed in in‐vitro studies. An initial, tremendous release was observed from both RF and INH drug formulated carriers, which was followed a regular prolonged releases. The outcome of the drug release was controlled by their drug quantity, indicating that the discharge could be suffered by concentration of drug. The results suggest that CS‐PCAR nanoparticles are a pretty system for simultaneous release of RF and INH in the treatment of tuberculosis.
Fluorescent N‐Heteroacene‐Based π‐Conjugated Liquid Responsive to HCl Vapor
Authors: Isoda Kyosuke , Sato Yuika , Matsukuma Daisuke 
Published: August 2017

AbstractIn this paper, we describe here the first room temperature π‐conjugated liquid based on N‐heteroacene framework showing a fluorescent property. This π‐conjugated liquid N‐heteroacene molecule is composed of branched‐long alkoxy chains and thiophene moieties. Also, N‐heteroacene‐based liquid itself can function as a stimuli‐responsive photofunctional material unlike room temperature π‐conjugated liquids reported previously. When a coated film prepared from this liquid was exposed to HCl vapor, an adsorption of HCl molecules occurred accompanied to the change in color of film from yellow to deep yellow. Moreover, emission colors of a coated film markedly changed from blue to yellow. This result indicates that this π‐conjugated liquid N‐heteroacene can function as stimuli‐responsive functional material.
Cobalt(II) Phthalocyanine Catalyzed One‐Pot Synthesis of 2‐Substituted Benzimidazoles, Benzothiazoles and Benzoxazoles from Substituted Benzyl Alcohols
Authors: Narang Uma , Yadav Kumar Karitkey , Bhattacharya Soumee , Chauhan Shive M. S. 
Published: August 2017

AbstractCobalt(II) phthalocyanine catalyzed one‐pot efficient synthetic procedure for the synthesis of 2‐substituted benzimidazoles, benzothiazoles and benzoxazoles has been developed by using molecular oxygen as an oxidant. Varieties of o‐substituted anilines were successfully used as the substrates for the current catalytic reaction and were transformed in good to excellent yields of products. Different electron rich and electron deficient benzyl alcohols are used as the substrates to give the excellent yield of products.