bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kallikrein-related peptidase 4 contributes to the tumor metastasis of oral squamous cell carcinoma
Authors: Cui Zhi , Cui Ye , Luo Gan , Yang Shuting , Ling Xinlian , Lou Yixin , Sun Xinhua 
Published: July 2017

Abstract
Oral squamous cell carcinoma (OSCC) is a disfiguring malignancy and significantly impacts the quality of patient’s life. Kallikrein-related peptidase 4 (KLK4), which is closely related to cancers, is highly expressed in OSCC. To explore the biological function of KLK4 in OSCC, a KLK4-specific shRNA was used to silence its endogenous expression, and then the migration and invasion of OSCC cells were explored. Results of our study showed that silencing KLK4 inhibited the migration and invasion of OSCC cells. The protein levels of epithelial mesenchymal transition-associated markers and proteases were also altered by KLK4 silencing. Further study showed that the phosphatidylinositol 3-kinase (PI3 K)/protein kinase B (AKT) signaling pathway was involved in the function of KLK4. Treatment with a PI3 K/AKT activator reversed the migration-inhibitory effect of KLK4 shRNA. Our study suggests that KLK4 may contribute to the metastasis of OSCC through the PI3 K/AKT signaling pathway.
A rigorous method to enrich for exosomes from brain tissue
Authors: Vella Laura J. , Scicluna Benjamin J. , Cheng Lesley , Bawden Emma G. , Masters Colin L. , Ang Ching‐Seng , Willamson Nicholas , McLean Catriona , Barnham Kevin J. , Hill Andrew F. 
Published: July 2017

ABSTRACTExtracellular vesicles, including exosomes, are released by all cells, including those of the nervous system. Capable of delivering lipid, protein and nucleic acids to both nearby and distal cells, exosomes have been hypothesized to play a role in progression of many diseases of the nervous system. To date, most analyses on the role of these vesicles in the healthy and diseased state have relied on studying vesicles from in vitro sources, such as conditioned cell culture media, or body fluids. Here we have taken a critical approach to the enrichment and characterization of exosomes from human frontal cortex. This method maintains the integrity of the vesicles and their cargo, and comprehensive proteomic and genomic characterization confirms the legitimacy of the resulting extracellular vesicles as endosome‐derived exosomes. This method will enable neuroscientists to acquire more detailed information about exosomes in the brain and explore the role(s) this form of intercellular communication and unique source of lipid, protein and RNA has in healthy brain function and pathogenic conditions. Furthermore, this method may have important utility in the isolation of exosomes from other tissues.
Sequence2Vec: a novel embedding approach for modeling transcription factor binding affinity landscape
Authors: Dai Hanjun , Umarov Ramzan , Kuwahara Hiroyuki , Li Yu , Song Le , Gao Xin 
Published: July 2017

Abstract

Motivation
An accurate characterization of transcription factor (TF)-DNA affinity landscape is crucial to a quantitative understanding of the molecular mechanisms underpinning endogenous gene regulation. While recent advances in biotechnology have brought the opportunity for building binding affinity prediction methods, the accurate characterization of TF-DNA binding affinity landscape still remains a challenging problem.

Results
Here we propose a novel sequence embedding approach for modeling the transcription factor binding affinity landscape. Our method represents DNA binding sequences as a hidden Markov model which captures both position specific information and long-range dependency in the sequence. A cornerstone of our method is a novel message passing-like embedding algorithm, called Sequence2Vec, which maps these hidden Markov models into a common nonlinear feature space and uses these embedded features to build a predictive model. Our method is a novel combination of the strength of probabilistic graphical models, feature space embedding and deep learning. We conducted comprehensive experiments on over 90 large-scale TF-DNA datasets which were measured by different high-throughput experimental technologies. Sequence2Vec outperforms alternative machine learning methods as well as the state-of-the-art binding affinity prediction methods.

Availability and implementation
Our program is freely available at https://github.com/ramzan1990/sequence2vec.

Supplementary information
Supplementary data are available at Bioinformatics online.
Twelve easy steps to embrace or avoid scientific petrification: lessons learned from a career in otolith research†
Authors: Campana Steven E 
Published: July 2017

Abstract
Prior experience is as much an eye opener in science as it is in life, and often results in increased efficiency, greater productivity and reduced stress. While some actions and behaviours must be experienced first-hand to be appreciated, there are some behavioural patterns that can be readily absorbed from others, allowing the learning curve to be shortened and the professional career enhanced. After nearly 40 years of scientific research in otolith and shark science, it is clear that some strategies and tactics worked well at advancing my career, while others were ineffective or even counterproductive. Targeted mainly at graduate students and early-to-mid-career scientists, this somewhat philosophical essay identifies 12 easily adopted scientific behaviour patterns that would have had a hugely positive effect on my career, if only I had known about them early on. My hope is that early-career scientists can take advantage of some of the hard lessons that I have learned along the way, thus avoiding needless mistakes in the process of becoming the best scientist that they can be.
Love and Economics
Authors: Marino Patricia 
Published: July 2017

Abstract
This chapter is a philosophical discussion of the relationship between love and economics. Because problems like the gender wage gap are analyzed through economic reasoning but also involve relations like love, and because economic methods are increasingly used to address a wide range of domains including family life, this relationship is important to understand. On the face of it, there is a tension between love, which is often thought to essentially involve caring and other-regarding preferences and attitudes, and the self-interestedness characteristic of neoclassical models of human behavior. The article analyzes various attempts to finesse the difficulty, such as Gary Becker’s characterization of altruism in terms of interdependent utility functions and Ann Cudd’s distinction between self-interestedness and selfishness, then draws on philosophical literature to examine implications of various approaches.
Consolidation and Challenge: 1990—Present
Authors: Best Thomas F. 
Published: July 2017

Abstract
The ecumenical movement exists to make the unity of Christ’s church more visible and effective in the churches’ own lives, and in their common witness and service to the world. Since 1990 it has made remarkable progress in such areas as ecclesiology, worship, mission, prophetic witness, and service in global, regional, national, and local contexts. Not least, it has broadened its scope as new partners have come to the table. Churches, councils of churches, bilateral discussions, united and uniting churches, and church aid and development agencies have all contributed to this success. But while ecumenism is now embedded in the lives of many churches, it faces challenges posed by the churches’ declining resources, their growing focus on internal issues, and the general societal distrust of organizations beyond the local level. Notions such as mutual accountability may help the churches live out their ecumenical vocation.
Perioperative Behavioral Therapy and Pelvic Muscle Strengthening Do Not Enhance Quality of Life After Pelvic Surgery: Secondary Report of a Randomized Controlled Trial
Authors: Weidner Alison C. , Barber Matthew D. , Markland Alayne , Rahn David D. , Hsu Yvonne , Mueller Elizabeth R. , Jakus-Waldman Sharon , Dyer Keisha Y. , Warren Lauren Klein , Gantz Marie G. , Meikle Susie 
Published: July 2017

Abstract

Background
There is significant need for trials evaluating the long-term effectiveness of a rigorous program of perioperative behavioral therapy with pelvic floor muscle training (BPMT) in women undergoing transvaginal reconstructive surgery for prolapse.

Objective
The purpose of this study was to evaluate the effect of perioperative BPMT on health-related quality of life (HRQOL) and sexual function following vaginal surgery for pelvic organ prolapse (POP) and stress urinary incontinence (SUI).

Design
This study is a secondary report of a 2 × 2 factorial randomized controlled trial.

Setting
This study was a multicenter trial.

Participants
Participants were adult women with stage 2–4 POP and SUI.

Intervention
Perioperative BPMT versus usual care and sacrospinous ligament fixation (SSLF) versus uterosacral ligament suspension (ULS) were provided.

Measurements
Participants undergoing transvaginal surgery (SSLF or ULS for POP and a midurethral sling for SUI) received usual care or five perioperative BPMT visits. The primary outcome was change in body image and in Pelvic Floor Impact Questionnaire (PFIQ) short-form subscale, 36-item Short-Form Health Survey (SF-36), Pelvic Organ Prolapse-Urinary Incontinence Sexual Questionnaire short form (PISQ-12), Patient Global Impression of Improvement (PGII), and Brink scores.

Results
The 374 participants were randomized to BPMT (n = 186) and usual care (n = 188). Outcomes were available for 137 (74%) of BPMT participants and 146 (78%) of the usual care participants at 24 months. There were no statistically significant differences between groups in PFIQ, SF-36, PGII, PISQ-12, or body image scale measures.

Limitations
The clinicians providing BPMT had variable expertise. Findings might not apply to vaginal prolapse procedures without slings or abdominal apical prolapse procedures.

Conclusions
Perioperative BPMT performed as an adjunct to vaginal surgery for POP and SUI provided no additional improvement in QOL or sexual function compared with usual care.
Transience, Class and Gender in Interwar Sexual Health Policy: The Case of the Liverpool VD Scheme
Authors: Caslin Samantha 
Published: July 2017

Abstract
Focusing on the implementation of the Liverpool VD Scheme, this article reasserts the importance of morality to interwar medical understandings about the spread of venereal disease. Despite claiming to offer impartial, practical solutions to the spread of venereal disease, the Liverpool VD Scheme, created in 1916, reflected and promoted the notion that the transient lifestyles of many of the working class presented physical and moral threats to the city. This article therefore counters suggestions that the interwar control of venereal disease was shaped by practicalities rather than moralities. Evidence is provided for the persistence of a medico-moralising that continued to place working-class sexual practices at the heart of discussions about the spread of venereal disease. However, presumptions about men’s biological need for sex combined with the local importance of the port, meaning that working-class seamen with VD were judged less harshly than working-class women with the same infections.
An unusual case of acute parotitis in a young adult
Authors: Sehic Azra , Haenig Caitlin , Spear Francis 
Published: July 2017

ABSTRACT
Acute bacterial parotitis is uncommon in young adults. Infection with Serratia marcescens is even rarer and usually found in hospitalized patients. This case report focuses on a young woman with acute bacterial parotitis caused by S. marcescens that required a longer-than-normal course of antibiotics.
Impact of Mindfulness-Based Parenting on Women in Treatment for Opioid Use Disorder
Authors: Gannon Meghan , Mackenzie Michael , Kaltenbach Karol , Abatemarco Diane 
Published: July 2017

Objectives:
Mothers with opioid use disorder are at high risk for maladaptive parenting. The present observational study aimed to measure the impact of a trauma-informed mindfulness-based parenting (MBP) intervention on quality of parenting behaviors of mothers primarily with opioid use disorders as well as examine associations between exposure to adverse childhood experiences and self-reported mindful parenting.

Methods:
A pretest posttest design was used with repeated measures. A total of 160 women were recruited from a substance use treatment program into the 12-week-long group-based intervention comprised didactic and experiential mindfulness activities. The Keys to Interactive Parenting Scale (KIPS) measured quality of parenting behavior, the Adverse Childhood Experiences Tool captured history of exposure to childhood trauma, and the Interpersonal Mindfulness in Parenting (IM-P) scale measured the degree of mindful parenting. Analyses were conducted using multilevel modeling.

Results:
The MBP intervention resulted in clinically significant improvements in KIPS total and all subscale scores and an IM-P total score. Data showed higher baseline Adverse Childhood Experiences and higher program attendance significantly predicted improved overall quality of parenting behaviors at a greater rate over time. Higher IM-P scores were associated with greater rate of improvement in KIPS total and all subscale scores.

Conclusions:
Study findings suggest a trauma-informed MBP intervention for parenting women with opioid use disorders is associated with significant clinical improvements in quality of parenting behavior. Results of this model show promise in supporting parenting of mothers receiving treatment for opioid use disorders to enhance bonding and parenting.
Large Eyelid Defect Repair Using a Free Full-Thickness Eyelid Graft
Authors: Memarzadeh Khashayar , Engelsberg Karl , Sheikh Rafi , Malmsjö Malin 
Published: July 2017

Summary:
Large eyelid full-thickness defects are traditionally repaired using flaps with a blood-supplying pedicle, for the reconstruction of the anterior or posterior lamella or both. This is a 2-stage procedure involving occlusion of vision in the affected eye for 4–8 weeks, as the flap pedicle is not divided until vascularization is deemed adequate. However, the importance of using a flap with a pedicle to ensure adequate perfusion of the graft has recently been questioned.
Autologous Latissimus Dorsi Breast Reconstruction Flap Salvage: Microvascular Anastomosis with Serratus Branch
Authors: Kuta Victoria , LeBlanc Martin 
Published: July 2017

Summary:
Autologous breast reconstruction has become a standard option during the recovery of breast cancer survivors. Although pedicle damage is a rare complication of this procedure, extensive torsion or tension can lead to partial or total flap failure. We report a case of partial flap salvage after accidental transection of the pedicled blood supply within the intramuscular course of a latissimus dorsi musculocutaneous flap. This salvage technique involved microvascular anastomosis between the remaining vasculature of the latissimus dorsi pedicle and the serratus branch of the thoracodorsal artery and vein.
Comparative Study of Prepectoral and Subpectoral Expander-Based Breast Reconstruction and Clavien IIIb Score Outcomes
Authors: Bettinger Lynne N. , Waters Linda M. , Reese Stephen W. , Kutner Susan E. , Jacobs Daniel I. 
Published: July 2017

Background:
Prepectoral breast reconstruction is increasingly popular. This study compares complications between 2 subpectoral and 1 prepectoral breast reconstruction technique.

Methods:
Between 2008 and 2015, 294 two-staged expander breast reconstructions in 213 patients were performed with 1 of 3 surgical techniques: (1) Prepectoral, (2) subpectoral with acellular dermal matrix (ADM) sling (“Classic”), or (3) subpectoral/subserratus expander placement without ADM (“No ADM”). Demographics, comorbidities, radiation therapy, and chemotherapy were assessed for correlation with Clavien IIIb score outcomes. Follow-up was a minimum of 6 months.

Results:
Surgical cohorts (n = 165 Prepectoral; n = 77 Classic; n = 52 No ADM) had comparable demographics except Classic had more cardiac disease (P = 0.03), No ADM had higher body mass index (BMI) (P = 0.01), and the Prepectoral group had more nipple-sparing mastectomies (P < 0.001). Univariate analysis showed higher expander complications with BMI ≥ 40 (P = 0.05), stage 4 breast cancer (P = 0.01), and contralateral prophylactic mastectomy (P = 0.1), whereas implant complications were associated with prior history of radiation (P < 0.01). There was more skin necrosis (P = 0.05) and overall expander complications (P = 0.01) in the Classic cohort, whereas the No ADM group trended toward the lowest expander complications among the 3. Multivariate analysis showed no difference in overall expander complication rates between the 3 groups matching demographics, mastectomy surgery, risks, and surgical technique.

Conclusions:
Prepectoral and subpectoral Classic and No ADM breast reconstructions demonstrated comparable grade IIIb Clavien score complications. BMI > 40, stage 4 cancer, and contralateral prophylactic mastectomy were associated with adverse expander outcomes and a prior history of radiation therapy adversely impacted implant outcomes. Ninety-day follow-up for expander and implant complications may be a better National Surgical Quality Improvement Program measure.
Percutaneous endoscopic gastrostomy: confirming the clinical benefits far beyond anthropometry
Authors: Cúrdia Gonçalves T. , Marinho Carla , Magalhães Joana , Barbosa Mara , Monteiro Sara , Dias de Castro Francisca , Boal Carvalho Pedro , Rosa Bruno , Figueiredo Lília , Cotter José 
Published: July 2017

Objectives
The real benefit of gastrostomy is still a matter of debate. We aimed to prospectively evaluate the global impact of percutaneous endoscopic gastrostomy (PEG) in patients followed at a specialized multidisciplinary clinic, namely, the impact on the need for healthcare resources, anthropometric measures, pressure ulcers prevention and healing, and nutritional and hydration status.

Patients and methods
From the 201 patients who underwent PEG between May 2011 and September 2014, 60 were included in a prospective study. Anthropometric, clinical, and laboratorial variables were collected and compared before and after PEG. Follow-up duration, mortality, and number of emergency department visits or hospital admissions were also assessed.

Results
Thirty-three (55.0%) patients were women and the median age was 79 years. The main indications for PEG were dementia (43.3%) and poststroke dysphagia (30.0%). Four months following PEG, significant decreases in the tricipital skinfold (P=0.002) and brachial perimeter (P=0.003) were found. A decrease in the mean number of hospitalizations (1.4 vs. 0.3; P<0.001) and visits to emergency department (2.2 vs. 1.1; P=0.003) was noted in the next 6 months after PEG compared with the previous semester. In 53.8% of patients with pressure ulcers, complete healing was observed after PEG. PEG was associated with increases in hemoglobin (P=0.024), lymphocytes (P=0.041), cholesterol (P=0.008), transferrin (P<0.001), albumin (P<0.001), and total proteins (P<0.001), and a decrease in serum sodium (P=0.001).

Conclusion
Anthropometric values may not translate the early benefits of a gastrostomy. PEG decreases the need for hospital health care, facilitates healing of pressure ulcers, and induces biochemical changes that may reflect better nutrition and hydration.
Development of a prognostic nomogram for cirrhotic patients with upper gastrointestinal bleeding
Authors: Zhou Yu-Jie , Zheng Ji-Na , Zhou Yi-Fan , Han Yi-Jing , Zou Tian-Tian , Liu Wen-Yue , Braddock Martin , Shi Ke-Qing , Wang Xiao-Dong , Zheng Ming-Hua 
Published: July 2017

Background and aim
Upper gastrointestinal bleeding (UGIB) is a complication with a high mortality rate in critically ill patients presenting with cirrhosis. Today, there exist few accurate scoring models specifically designed for mortality risk assessment in critically ill cirrhotic patients with upper gastrointestinal bleeding (CICGIB). Our aim was to develop and evaluate a novel nomogram-based model specific for CICGIB.

Patients and methods
Overall, 540 consecutive CICGIB patients were enrolled. On the basis of Cox regression analyses, the nomogram was constructed to estimate the probability of 30-day, 90-day, 270-day, and 1-year survival. An upper gastrointestinal bleeding–chronic liver failure–sequential organ failure assessment (UGIB–CLIF–SOFA) score was derived from the nomogram. Performance assessment and internal validation of the model were performed using Harrell’s concordance index (C-index), calibration plot, and bootstrap sample procedures. UGIB–CLIF–SOFA was also compared with other prognostic models, such as CLIF–SOFA and model for end-stage liver disease, using C-indices.

Results
Eight independent factors derived from Cox analysis (including bilirubin, creatinine, international normalized ratio, sodium, albumin, mean artery pressure, vasopressin used, and hematocrit decrease>10%) were assembled into the nomogram and the UGIB–CLIF–SOFA score. The calibration plots showed optimal agreement between nomogram prediction and actual observation. The C-index of the nomogram using bootstrap (0.729; 95% confidence interval: 0.689–0.766) was higher than that of the other models for predicting survival of CICGIB.

Conclusion
We have developed and internally validated a novel nomogram and an easy-to-use scoring system that accurately predicts the mortality probability of CICGIB on the basis of eight easy-to-obtain parameters. External validation is now warranted in future clinical studies.
Characteristics of Patients Involved in Physical Assault in an Acute Inpatient Psychiatric Setting
Authors: SANGHANI SOHAG N. , MARSH AKEEM N. , JOHN MAJNU , SOMAN ARYA , LOPEZ LEONARDO V. , YOUNG YOLANA A. , RUSS MARK J. 
Published: July 2017

Background:
Although aggressive behavior in psychiatric settings is a major concern, very few studies have focused exclusively on physical assault in a general inpatient psychiatric population.

Objectives:
This study had 3 main goals: (1) to evaluate the prevalence of assaultive behavior in an acute psychiatric hospital; (2) to identify the clinical and socio-demographic factors associated with assaultive behavior during hospitalization; and (3) to explore whether a diagnosis of schizophrenia spectrum disorder increases the risk of assaultive behavior.

Methods:
We conducted a retrospective chart review of patients admitted to acute units in a psychiatric hospital between 2009 and 2012. A subset of occurrence reports identified by a multidisciplinary team as “physical assault” was included in the analysis. Using logistic multivariate regression analysis, these patients were compared with a randomly selected nonassaultive control group, matched for length of stay to identify factors associated with assaultive behavior.

Results:
Of 757 occurrence reports, 613 met criteria for significant assault committed by 356 patients over 309,552 patient days. The assault incident density was 1.98 per 1000 patient days. In the logistic regression model of best fit, the factors significantly associated with assaultive behavior were age, legal status, and substance use. A diagnosis of schizophrenia spectrum disorder was not significantly associated with assaultive behavior.

Conclusions:
Clinicians should take extra precautions for involuntarily admitted young patients with a history of substance use, as they are more likely to exhibit assaultive behavior. A diagnosis of schizophrenia spectrum disorder in itself is not significantly associated with assaultive behavior. Screening instruments such as the Dynamic Appraisal of Situational Aggression may be useful in assessing risk of assault.