bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine Perfusion or Straight to Transplant? Predictive Value of Flavin Mononucleotide Levels in Flush Solution of Human Liver Allograft
Authors: Ali Khaled , Cazzaniga Beatrice , Liu Qiang , Miyazaki Yuki , Tuul Munkhbold , Raj Roma , Hussein Ahmed , Wehrle Chase , Zhang Mingyi , Calderon Esteban , Kusakabe Jiro , Shanmugarajah Kumaran , Wakam Glenn , Khalil Mazhar , Pita Alejandro , Aucejo Federico , MD Choon David Kwon , Kim Jaekun , Fujiki Masato , Miller Charles , Quintini Cristiano , Schlegel Andrea , Hashimoto Koji 
Published: October 2024

Objective:
This study examined the predictive value of Flavin Mononucleotide (FMN) levels in the flush solution used during cold storage of donor livers on outcomes post-transplantation.

Background:
Static cold storage for liver grafts induces hypoxia with subsequent impaired mitochondrial function and Flavin Mononucleotide (FMN) release upon reperfusion.

Methods:
This study enrolled 62 recipients who received whole liver grafts from donation after brain death (n=50) and circulatory death donors (n=12) between June 2022 and July 2023. FMN concentrations were measured in flush solutions on the back-table. ROC-curve analysis identified an FMN level cut-off for graft survival. Post-transplant outcomes were examined according to FMN levels.

Results:
FMN level was significantly associated with graft survival, with an area-under-the-curve (AUC) of 0.858 (95%CI: 0.754-0.963, P<0.001), outperforming the donor risk index (AUC 0.571, 95%CI: 0.227-0.915, P=0.686). The study cohort was divided into low-FMN (<37.5 ng/mL, n=40) and high-FMN groups (≥37.5 ng/mL, n=22). The low-FMN group had superior one-year graft survival compared with the high-FMN group (100% vs. 77%, P=0.003). Levels of transaminases within 7 days post-transplant were significantly higher in the high-FMN group (P=0.003). The high-FMN group developed acute rejections (41% vs. 15%, P=0.023) and early allograft dysfunction (50% vs. 20%, P=0.014) more frequently. Median comprehensive complication index in the high-FMN group was significantly higher (54 [interquartile range, 40-78] vs. 42 [interquartile range, 28-52], P=0.017).

Conclusion:
The FMN level measured in donor livers’ cold storage flush solution is a valid biomarker to predict post-transplant outcomes. Liver grafts with high FMN levels may benefit from machine perfusion to improve outcomes.
Barley is a potential trap crop for root parasitic weeds broomrapes
Authors: Inoue Maiko , Xie Xiaonan , Yoneyama Kaori 
Published: October 2024

ABSTRACTRoot parasitic weeds broomrapes (PhelipancheandOrobanchespp.) cause devastating damages to agricultural production all over the world. The seeds of broomrapes germinate when they are exposed to germination stimulants, mainly strigolactones (SLs), released from roots of any plant species, while broomrapes parasitize only dicot plants. Therefore, monocots can be trap crops for broomrapes, as they induce seed germination but are not parasitized. In this study, we examined two European and one Japanese cultivars of barley for their potentials as trap crops for broomrapes and found that the European cultivars, Sebastian and Golden promise, are good potential trap crops as they produce more SLs and exhibit higher mycorrhizal colonization rates as compared to the Japanese cultivar Shunrai.
Regulation of the Promoter for Capsular Polysaccharide Synthesis inNeisseria meningitidisSerogroup B by HTH_XRE Family Transcription Factor
Authors: Sofowora Iyinoluwa , McCarthy Pumtiwitt , Wachira James 
Published: October 2024

AbstractThe capsular polysaccharide synthesis (cps) locus ofNeisseria meningitidisis implicated in invasive meningococcal disease. The synthesis (synABCD) and transport (ctrABCD) operons are transcribed in opposite directions from a common intergenic region and expression is negatively regulated by the bacterial two-component systemmisR/misSand thermosensitive RNA folding. However, these mechanisms do not fully explain the stationary phase responses and the cis-acting elements remain to be fully characterized. Using GFP reporter gene and site-directed mutagenesis, cis-regulatory elements in the 134-bp intergenic region, NmIR, were investigated. While confirming a known RpoD promoter, an additional potential promoter element and putative binding sites for the transcription factorsfis and lexAwere identified through sequence analysis. Deletion of the putative LexA binding site led to an increase in GFP fluorescence. TheN. meningitidis genome carries only one lexA homolog, theHelix-Turn-Helix regulator XRE family member (GenBank-NMB0910, HTH_XRE). Trans-complementation of the NmIR-GFP reporter with theN. meningitidisHTH_XRE expression plasmid led to increased fluorescence. Trans-complementation with eithermisR/misSornusGdecreased reporter gene expression. Consistent with previous reports, deletion of the RpoD promoter reduced expression by 50%, suggesting a redundancy of promoter elements in the intergenic region. Thus, the results confirm the functioning of an exogenousN. meningitidisCPS synthesis promoter inE. coliand demonstrate its regulation through trans-complementation bymisR/misS,HTH_XRE, andnusG.ImportancePathogenicNeisseria meningitidis, a causal agent of bacterial meningitis, secretes capsular polysaccharides of different compositions that differentiate the serogroups. Since the capsule is an important virulence factor that determines adhesion to epithelia and ability to invade tissues, there is need to understand the underlying mechanisms for its expression. Furthermore, bacterial polysaccharides are potential sources of novel biomaterials. The expression of the capsule production genes is regulated, and this study reveals a mechanism involving a transcription factor, HTH_XRE, whose function inNeisseria meningitidisis not known. It extends the understanding of capsular expression regulation by identifying other control elements in the promoter region. The results will have applications in optimizing biomaterial production or in developing therapeutic interventions.
Network analysis of cross-income-level collaboration on non-communicable disease: the example of multiple myeloma in Sub-Saharan Africa
Authors: Yang Kaiyi , Benkwitz-Bedford Sam , Cazier Jean-Baptiste , Spill Fabian 
Published: October 2024

AbstractCross-income-level collaboration (CILC) is crucial for developing global health approaches that benefit low- and middle-income countries (LMICs). Multiple myeloma (MM) is a representative example of a complex, understudied disease in sub-Saharan Africa (SSA). Based on publications, we developed a network analysis tool to assess scientific collaborations. Here, we present findings from a systematic analysis of publications retrieved from PubMed between January 2002 and June 2022. We evaluated individual institutional contributions and collaboration patterns using undirected weighted networks. Our findings reveal that intraincome-level collaborations dominate MM research in SSA, with high-income countries (HICs) primarily engaging with a few local institutions, mainly in South Africa and Nigeria. Increasing CILC is essential to advance research in this area. Our analysis tool provides insights into the collaboration strength, highlights gaps in the field and identifies leading institutions, ultimately aiming to support the development of more effective international collaboration and research strategies in MM.
The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
Authors: Gonzalo Lucia , Gagliardi Delfina , Zlauvinen Camila , Gulanicz Tomasz , Arce Agustín L. , Fernandez Josefina , Cambiagno Damian A. , Zienkiewicz Agnieszka , Jarmolowski Artur , Manavella Pablo A. 
Published: October 2024

AbstractThe regulation of miRNA biogenesis and movement is essential for plant development and environmental responses. HASTY (HST), a karyopherin protein, has been implicated in miRNA biogenesis and movement, though its role in non-cell-autonomous miRNA movement remains unclear. Through a genetic screen, we identified that mutations in theHAWAIIAN SKIRT (HWS)gene suppress the developmental defects ofhstmutants by restoring miRNA movement. Our findings show that HWS interacts with nuclear transport factors and nuclear pore complex (NPC) components, including NUP1, positioning HWS as a regulator of miRNA nuclear export. Using microscopy and fluorescencein situhybridization, we showed that pri-miRNA transcription, and likely their co-transcriptional processing, occurs at the nuclear pore. Notably, we uncovered an antagonistic relationship between HST and HWS in regulatingMIRNAtranscription at the NPC and AGO1 loading, which could explain the observed changes in miRNA movement. HST promotes the association ofMIRNAloci with the NPC, likely facilitating co-transcriptional processing, while HWS negatively regulates this process by targeting MEDIATOR complex subunits to degradation detaching, consequently, the processing complex from the NPC. Furthermore, our data provides evidence of spatial coordination of miRNA transcription, biogenesis, and movement highlighting a novel role for the NPC in the miRNA pathway.
Application of the maturity model for collaborative scheduling for construction projects
Authors: Scala Natalie M. , Alves Thais da Costa Lago , Hawkins Dominique , Schiavone Vincent , Liu Min 
Published: October 2024

PurposeThis paper introduces the weighting, analysis and validation method used in the development of the Maturity Model for Collaborative Scheduling (MMCS). The scoring and ranking process introduced by the MMCS fills a gap in the literature by supporting the selection of collaborative scheduling (CS) practices that yield more weight toward the achievement of higher maturity levels in the development and implementation of CS. The ranking process can then be used during pre/post project execution to track collaborative scheduling in practice against the model’s weighting and provide the project team with constructive feedback and actionable steps for reaching the next highest level of collaboration.Design/methodology/approachThe MMCS, which focuses on five pillars (key areas of interest for CS) and related swim lanes (specific attributes), covers a broad range of areas in the construction industry and was coded into a survey. The relative weights of pillars and swim lanes were then established using the Delphi method with the group of subject matter experts (SMEs), analyzed using multi-objective decision analysis (MODA) and validated using 241 answers to a survey with questions drawn from the MMCS, including organizations across the industry in the United States.FindingsThe project scoring defines bounds for bronze, silver and gold levels of collaboration in scheduling. Project evaluations can then be used to identify areas for continuous improvement and enhanced collaboration. We offer recommendations and best practices for project improvement.Originality/valueTwo original contributions resulted from this work: (1) a method to elicit weights based on a combination of Delphi, MODA and survey methods was used to develop and validate a scale with three different maturity levels to support the use and continuous improvement of CS practices and (2) a validated model was used to assess the maturity level of CS in construction projects alongside specific recommendations to move upward in terms of maturity. In practice, project leaders can use this model to assess project performance, advance the project’s maturity and guide continuous improvement efforts for enhanced collaboration.
From research to resources: developing a case-based learning curriculum for navigating clinical uncertainty
Authors: Lundgren Henriette , Papanagnou Dimitrios , Morrone Casey , Vaid Urvashi , Ghei Ridhima , Bierowski Abagayle , Watkins Karen E. , Marsick Victoria J. 
Published: October 2024

Purpose
This study aimed at rethinking ways in which educators from different fields can collaborate to respond to the rapidly evolving demands of health professions education (HPE). The goal was to investigate how a research-to-resources approach can be applied to engage in knowledge translation (KT) of research findings for the benefit of introducing medical students to uncertainty in the clinical learning environment.

Design/methodology/approach
An interdisciplinary team of medical educators, human resource development (HRD) scholars and emergency medicine fellows engaged in iterative cycles of action research (AR) to develop, pilot and refine case-based learning resources on clinical uncertainty. The team leveraged prior research on physicians’ decision-making during COVID-19, experimented with generative AI tools, and collected feedback from medical students to guide resource development.

Findings
The findings of this study are twofold. On the one hand, the authors reflect on the lessons learned of developing case-based learning with the help of generative AI. While student feedback indicated that the case helped normalize and process experiences with uncertainty, key challenges included adapting research data to create relevant, sustainable learning resources and designing effective discussion prompts. On the other hand, the authors provide insights into the opportunities and challenges of our interdisciplinary collaboration. The authors show that knowledge utilization is not simple, but complex, and that more work needs to be done to effectively disseminate resources as part of the desired uncertainty curriculum.

Practical implications
This study attempts to apply a KT framework for bridging the research-practice gap in HPE through interdisciplinary collaboration and AR. It provides lessons learned for developing case-based curricula on complex topics like uncertainty. The findings highlight the need for adaptive KT processes when dealing with rapidly evolving healthcare contexts.

Originality/value
This paper offers a novel example of research-to-resource KT in medical education, integrating perspectives from HRD and leveraging emerging technologies. It contributes to understanding how interdisciplinary teams can collaborate to create timely, evidence-based educational resources for navigating uncertainty in professional practice. The study also provides insights into the challenges and opportunities of translating complex research findings into practical learning tools to tackle real-world challenges in HPE.
The contribution of public libraries in strengthening digital citizenship in Egyptian society – an exploratory study
Authors: Hassan Amr Hassan Fatouh , Shehata Ahmed 
Published: October 2024

Purpose
This study aims to assess the involvement of Egyptian public libraries in supporting digital citizenship and propose a vision to leverage their role in promoting digital citizenship culture, aligning with Egypt Vision 2030 for sustainable development.

Design/methodology/approach
A questionnaire was used to sample 21 public library managers in Egypt. The study adopted a descriptive-analytic approach, measuring library readiness infrastructure, strategic planning for digital citizenship support and participation ratings on nine dimensions of digital citizenship as defined by the Ribble model.

Findings
Results indicated a moderate level of participation overall (average score of 1.72), with high levels in dimensions like digital access and digital literacy but much lower levels in others, particularly digital law and digital health and safety. Library readiness was found to have a positive effect on the level of digital citizenship promotion.

Research limitations/implications
The small sample size may limit generalizability. Future research should explore best practices for digital citizenship programming in libraries and strategies for overcoming implementation barriers.

Practical implications
The study underscores the need for Egyptian public libraries to enhance their readiness and participation in promoting digital citizenship. This requires investment in digital resources, staff training, community outreach and collaboration with educational institutions and community partners.

Originality/value
This study provides insights into the role of public libraries in promoting digital citizenship in the context of a developing country’s national vision. It offers a framework for libraries worldwide to assess and enhance their digital citizenship initiatives.
Does empowering leadership behavior affect employee performance? The mediating role of workforce agility
Authors: Varshney Deepanjana , Varshney Nirbhay Krishna 
Published: October 2024

PurposeWorkforce agility (WFA) is an emergent research topic in volatile times. However, there is a lack of research in understanding the leadership dimension that triggers such an attribute in organizations. Our study aims to understand the impact of workforce agility on empowering leadership behavior and employee performance dimensions (task performance, contextual performance and counterproductive work behavior).Design/methodology/approachWe collected data from 236 employees using reliable, validated scales and conducted various statistical analyses.FindingsOur results demonstrated that WFA (1) partially mediated the relationship between empowering leadership and contextual performance (CP), (2) has not mediated the relationship between empowering leadership and counterproductive behavior (CWB) and (3) mediated the relationship between empowering leadership and task performance (TP).Practical implicationsOur research has practical implications for management practitioners. It suggests hiring and developing an agile workforce through appropriate training and development programs can significantly impact organizational performance. Furthermore, it provides insights into building leadership capabilities that sustain workforce agility practices, empowering leaders to make informed decisions.Originality/valueOur research fills a significant gap in the existing literature by exploring the effects of WFA on leadership and performance. This novel approach provides a fresh perspective on the dynamics of organizational behavior, making it a valuable addition to the field.
Online teaching competencies and teacher effectiveness in higher education: the mediating effect of perceived career support
Authors: Sokro Evans , Obuobisa-Darko Theresa , Okpattah Bernard 
Published: October 2024

PurposeOnline teaching competencies are critical skills required for effective teaching and learning in higher education. However, evidence regarding the impact of online teaching competencies on teacher performance and career satisfaction is minimal in the literature. This study draws on the TPACK model to understand how online teaching competencies influence teacher performance and career satisfaction.Design/methodology/approachThe study adopts a quantitative approach and collected data from 243 faculty in both public and private universities in Ghana. Structural equation modelling was used to examine the relationships among the study constructs.FindingsThe results show that the various dimensions of TPACK have a significant positive effect on teacher performance and career satisfaction. Furthermore, the mediation results reveal that neither perceived career support nor teacher attributes mediate the relationship between online teaching competencies, teaching performance and career satisfaction.Practical implicationsThis paper provides a better understanding of how context knowledge, pedagogical knowledge, pedagogical context knowledge, technology pedagogical knowledge, career support and teacher attributes affect teacher performance.Originality/valueThis study draws on the TPACK (context knowledge, pedagogical knowledge, pedagogical context knowledge and technology pedagogical knowledge) model and incorporates perceived career support and teacher attributes as essential characteristics within an emerging economy country context.
Debunking neuromyths: Pre‐service teachers' insights on autism spectrum disorder
Authors: Lacruz‐Pérez Irene , Pastor‐Cerezuela Gemma , Caurín‐Alonso Carlos , Morales‐Hernández Antonio José , Tárraga‐Mínguez Raúl 
Published: October 2024

AbstractTeachers' beliefs in certain neuromyths about neurodevelopmental disorders can negatively impact the educational inclusion of students who present them. This study aims to analyse the prevalence of neuromyths about the health and the emotional competences of people with autism spectrum disorder (ASD) in pre‐service teachers; and to assess the possible contribution of university training to constructing accurate knowledge about autism. This prevalence was compared in three groups of pre‐service teachers: 1st‐year students, 4th‐year students who will be regular teachers and 4th‐year students training to be special education specialists. Additionally, it was proposed to identify the topic with the most myths (health or emotional competences) and the most frequent myths. A validated instrument was completed by 167 pre‐service teachers. Overall, the 4th‐year specialist group had more correct answers and fewer doubts that the 4th‐year regular group, which had more correct answers than the 1st‐year group. The prevalence of errors was not statistically different among the three groups. Moreover, participants answered more questions and made more errors about emotional competences in autism than about health. Among the most frequent myths are those concerning empathy or the cause of ASD. Based on these results, some implications for pre‐service teacher education are discussed.