bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wild-Type p53 Overexpression in NPM1-Mutated AML: Potential Implications for Disease Biology and Therapy Response
Authors: Barone Paul , Dzedzik Siarhei , Kleinman Ashley , Chin Christopher , Meydan Cem , Valencia Itzel , Sugita Mayumi , Guzman Monica , Fein Joshua , Kaner Justin , Desai Pinkal , Roboz Gail , Mason Christopher , Patel Sanjay 
Published: October 2024

Abstract

Somatic mutations in nucleophosmin (NPM1) are key in defining a common subtype of acute myeloid leukemia (NPM1-AML), characterized by chromosomal stability and favorable therapeutic responses. However, some patients exhibit a suboptimal response to initial treatment, and relapses are common, highlighting the need for novel biomarkers. Notably, NPM1and TP53 mutations rarely co-occur in AML, where TP53 mutations correlate with aneuploidy and poor survival outcomes. Here, we present transcriptomic and proteomic evidence of unexpectedly high wild-type (WT) TP53/p53 expression in a subset of NPM1-AMLs at baseline. Analyses of the Beat AML cohort indicate that NPM1-AMLs generally express more TP53than NPM1/TP53-WT AMLs. Among seventy-four NPM1-AML samples, those with the lowest TP53 co-expression are enriched for downregulated signatures linked to DNA repair, apoptosis, and the cell cycle. By profiling thirty-three primary patient samples using multiplex immunofluorescence staining with single cell-based quantitative digital image analysis, we observed increased p53 expression in NPM1-mutant cells compared to WT cells. Importantly, patients with primary refractory disease showed low p53 co-expression at diagnosis. These findings suggest that WT-p53 might play a crucial role in the biological and clinical characteristics seen in NPM1-AML.
Data Analysis for Determining Causation of Regional Differences in Transportation Infrastructure on Carbon Emissions
Authors: Baglio Nolan 
Published: October 2024

Abstract

Transportation supports global connections in the world. Urban planning requires much thought about transportation to ensure connectivity and simplicity within a city. However, what should also be considered is the environmental impact of their decisions. There is variation in how each county approaches its development projects. These differences play a crucial role in their environmental impact. The research aims to understand how different types of transportation infrastructure impact carbon emissions. Each country was categorized by region, and the study looked into six different regions: North America, South America, Asia, Europe, Africa, and Oceania. The ideal features for predicting the carbon emissions per capita were determined using random forest regression, a machine-learning model that creates many decision trees to make predictions. The carbon emissions per capita with the percentage of roads paved, the feature deemed most valuable to predicting carbon emissions per capita by the random forest regressor, in each region was compared. With linear regression, a relationship was determined in each region to understand the variance from the line and the slope of the line and to know how the data progresses as the values increase. The results showed that economic and governmental factors were often at the root of environmental factors, and transportation infrastructure reflects economic strength. This was understood from both correlations between carbon emission changes and financial events, as well as from policy reviews of countries that were anomalies in the data, showing that they had differing policies regarding transportation. In this research, transportation infrastructure was proven essential to environmental factors. The study also indicates that the jobs of urban planners and road workers play a significant role in how a country approaches its carbon-neutral goals.
Clinical characteristics and outcomes of acute pancreatitis at St. Paul’s Hospital Millennium Medical College: A five-year retrospective study.
Authors: Bereded Fekadu Negash , Yirdaw Hilmineh , Temesgen Sisay 
Published: October 2024

Abstract

Background: Acute pancreatitis is characterized by excessive upper abdominal pain, nausea, vomiting, or a systemic inflammatory response syndrome characterized by fever, low blood pressure, and, in some cases progression to organ failure. It is one of the most prevalent gastrointestinal diseases requiring hospital admission. Because of the scarcity of data in Ethiopia, it is necessary to understand the clinical profile and outcome of acute pancreatitis for better management and intervention.
Methods: this was an institutional-based cross-sectional study design in which all patients admitted with a diagnosis of acute pancreatitis who fulfilled the inclusion criteria were included. The study involved a review of complete and documented records of these patients, using a structured format to collect data on various sociodemographic variables, clinical presentations, imaging findings, biochemical profile patterns, and outcomes. The data were cleaned and analyzed via SPSS version 26.
Results: The mean age at presentation was 36.89 ± 10.4 years and females accounted for 51.1% of the sample. The most common etiology was gallstones (57.8%), followed by alcoholic pancreatitis (26.7%). Only 3 patients died, and the majority of patients were discharged alive. The severity of pancreatitis and the presence of complications are associated with the outcome of acute pancreatitis.
Conclusion: Females are more affected by acute pancreatitis, in our study area, with the majority being affected by biliary stones and alcohol.
Immunohistochemical Expression of P63 and P40 in Squamous Cell Carcinoma of Head and Neck
Authors: Zahid Aima , Akhtar Raana , Hameed Samreen , Mughal Zunairah , Talat Haseeba , Muzaffar Aliya 
Published: October 2024

Background: Squamous cell carcinoma (SCC) in head and neck region has a high worldwide prevalence. Increased accuracy in diagnosis is achieved by immunohistochemical markers.
Objectives: To evaluate the immunohistochemical expression of p63 and p40 in SCC and correlate with histological grades to determine p40 as a better potential diagnostic immunohistochemical marker for SCC.
Methods: Descriptive cross-sectional study was conducted on eighty-three cases of SCC of head and neck from March 2020 to February,2022 at Pathology Department, KEMU, Lahore. Data including age, sex, risk factors and tumor site was collected. SCC grading was done on H&E stained tissue sections. Staining with p63 and p40 was performed and independently scored for percentage of tumor cells stained and intensity of nuclear staining. The results were compared using H-scores in all grades of SCC. Data was entered in Statistical Package for Social Sciences(SPSS-26). Quantitative variables like age were presented as mean ±SD. Qualitative variables as frequency and percentages. Correlation of two groups was done by applying Chi-square test. P value ≤0.05 was taken as significant.
Results: SCC was graded according to the W.H.O. grading system. All cases stained positive for p63 and p40. Strong nuclear staining by p40 was observed in 65.02% cases versus 42.17% by p63. The mean tumor area stained by p40 was greater than p63 (90.35% vs 75.5%) in all cases. A significant higher mean H-score for p40 (9.69±2.86) versus p63 (7.05±3.12) was observed as indicated by significant P-value. The mean H-score of p40 immunoreactivity was 10.11 and p63 was 6.00 in poorly differentiated SCC. Males comprised 74.70% cases whereas females 25.30% only. SCC in oral cavity was (96.39%) followed by oropharynx and larynx.
Conclusion: P40 immunohistochemical marker was better compared to P63 for the diagnosis of SCC particularly in poorly differentiated SCC.
Fotobiomodulación como coadyuvante en la reducción del dolor postoperatorio en la cirugía de terceros molares. Revisión sistemática
Authors: Hernández-López Angélica , Lorena-Olvera Mayra , Maldonado-Guereca Manuel de Jesús , Gómez Palacio-Gastélum Marcelo , Vargas-Chávez Nohé 
Published: October 2024

Introducción. La cirugía de los terceros molares se asocia normalmente a diferentes complicaciones, destacando el dolor postoperatorio como la principal. Comúnmente, la terapéutica se concentra en la prescripción de antiinflamatorios no esteroideos como coadyuvantes para minimizar este el dolor; sin embargo, los antiinflamatorios no esteroideos tienen numerosos efectos secundarios gastrointestinales, renales y hematológicos. Actualmente, en un esfuerzo por ofrecer el mejor tratamiento coadyuvante en el control del dolor postoperatorio que no genere efectos secundarios, se ha sugerido la implementación de la terapia de fotobiomodulación. Objetivo. Realizar una revisión sistemática sobre la efectividad de la implementación de la terapia de fotobiomodulación para la reducción del dolor postoperatorio después de la cirugía de los terceros molares. Materiales y métodos. Se realizó una búsqueda en 3 bases de datos electrónicas gratuitas: PubMed, ScienceDirect, Wiley Online Library. En estas, se incluyeron ensayos clínicos aleatorizados publicados del 2015 al 2021. Resultados. Se encontró un número bajo de artículos, ninguno contó con un protocolo estandarizado de TFBM, además de no presentar concordancia entre las características de la aplicación de TFBM (longitud deonda, densidad de potencia medida, j/cm2 energía por punto); sin embargo, de los estudios incluidos todos arrojaron una significancia estadística en la disminución del dolor al aplicar TFBM.Conclusión. De acuerdo a los resultados obtenidos en esta revisión, la aplicación de la terapia de fotobiomodulación es efectiva para reducir el dolor postoperatorio después de la cirugía de terceros molares.Palabras clave: Fotobiomodulación, terapia laser de baja intensidad, terceros molares, dolor.
Penguatan Kapasitas Kelembagaan Sanggar Budaya Desa Kolowa: Pendampingan Pendirian Badan Hukum dan Penyusunan Kontrak Kerja
Authors: Abdullah La Ode Dedi , Pratiwi Endang Tri , Jamil Jamil , Mela Asmaul Soepardy 
Published: October 2024

Penelitian ini bertujuan untuk mendampingi SDM Sanggar Budaya Desa Kolowa dalam proses pendirian sanggar berbadan hukum dan penyusunan kontrak kerja. Metode yang digunakan adalah pendekatan kualitatif dengan teknik pengumpulan data melalui wawancara, observasi, dan studi dokumentasi. Program pendampingan dilakukan melalui workshop, pelatihan, dan konsultasi. Hasil penelitian menunjukkan bahwa proses pendirian lembaga berbadan hukum dimulai dengan pembuatan akta pendirian di hadapan notaris, dilanjutkan dengan pengesahan oleh Menteri, pendaftaran, dan pengumuman dalam Tambahan Berita Negara. Dalam penyusunan kontrak kerja, tahapan yang perlu dipahami meliputi pengertian kontrak, unsur-unsur perjanjian, jenis kontrak, isi kontrak, serta hak dan tanggung jawab para pihak. Implikasi dari penelitian ini adalah peningkatan kapasitas kelembagaan Sanggar Budaya Desa Kolowa yang diharapkan dapat mendukung pelestarian dan pengembangan kesenian lokal.

This study aims to assist the human resources of the Kolowa Village Cultural Studio in the process of establishing a legal entity and drafting a work contract. The method used is a qualitative approach with data collection techniques through interviews, observations, and documentation studies. The mentoring program is carried out through workshops, training, and consultations. The results of the study indicate that the process of establishing a legal entity begins with the preparation of a deed of establishment before a notary, followed by ratification by the Minister, registration, and announcement in the State Gazette Supplement. In drafting a work contract, the stages that need to be understood include the definition of a contract, elements of an agreement, types of contracts, contents of the contract, and the rights and responsibilities of the parties. The implication of this study is the increase in the institutional capacity of the Kolowa Village Cultural Studio which is expected to support the preservation and development of local arts.
Comportamiento tributario: factor clave de crecimiento económico y social
Authors: Soto-Arévalo Fanny Stella , Sánchez-Pérez María Angélica , Blanco-Gentil Jeison Rafael 
Published: October 2024

Los impuestos constituyen la parte más representativa de los ingresos fiscales, por ende, es indispensable que los contribuyentes se responsabilicen del cumplimento de sus obligaciones tributarias. El buen comportamiento tributario por parte de los empresarios es un factor clave que le permite al Estado proveer bienes y servicios y el desarrollo económico y social, es así como el presente artículo se enfoca en el análisis del comportamiento tributario por parte de 54 comerciantes, a partir de una investigación cuantitativa bajo un enfoque descriptivo, aplicando como instrumento de recolección de información el cuestionario, donde los resultados del estudio permitieron evaluar la actitud tributaria de los comerciantes y la confianza de los contribuyentes con el estado.
Age of neutering contributes to risk of cruciate ligament rupture in Labrador Retrievers
Authors: DeForge Teagan L. , Momen Mehdi , Conidi Gabi , Muir Peter , Sample Susannah J. 
Published: October 2024

Abstract

OBJECTIVE
Cruciate ligament rupture (CR) in Labrador Retrievers is a complex polygenic disease with high heritability. The environmental contribution to CR risk remains poorly characterized. An accurate genetic risk test for CR in the Labrador Retriever has been developed. This enables evaluation of environmental risk with knowledge of genetic disease predisposition through study of dogs with phenotypic disease status that is discordant with their genetic risk. The objective of this study was to identify environmental factors that contribute to CR in Labrador Retrievers through evaluation of dogs with clinical phenotypes that are discordantly predicted with the use of genetic markers.

METHODS
Dogs were prospectively recruited between January 2013 and December 2022. To study discordant subjects, case dogs with a posterior risk probability value < 0.75 and control dogs with a posterior risk probability of > 0.25, determined with the use of an average of 8 statistical models, were selected. The environmental factors investigated were neuter status, age of neuter, withers height measured at the dorsal-most ridge between the scapulae, weight, body mass index, and athletic activity.

RESULTS
Ninety three dogs were discordant: 58 dogs were discordant CR cases, and 35 dogs were discordant CR controls. Neutering before 12 months of age was a significant risk factor for CR development. Sex, neuter status, or status as an athlete was not associated with CR risk.

CONCLUSIONS
Neutering before 12 months of age influences risk of CR in Labrador Retrievers.

CLINICAL RELEVANCE
This information can inform management decisions about Labrador Retrievers regarding age of neutering, body condition, and athletic activity. The primary factor influencing CR development in Labrador Retrievers is polygenic intrinsic genetic risk.
Study of Dyeing With Natural Extract of Butterfly Pea Flowers (Clitoria Ternatea) on Cotton Fabrics
Authors: Hangai Julie Namie Ono , Vila Nívea Tais , Ferreira Alexandre Jose Sousa , Silva Marcia Gomes da 
Published: October 2024

Objective: This study aims to optimize the dye extraction and dyeing variables in cotton fabrics using dried Butterfly Pea flowers (Clitoria ternatea), in order to evaluate its potential as a natural dye in the dyeing of cotton fabrics.

Theoretical Framework: This research is presented based on fundamentals related to dyeing with natural dyes, dye extraction and pre-mordanting procedures, as well as studies related to the dyeing of cotton fabrics.

Method: This research was carried out by varying the dye extraction temperature and dyeing parameters, which were documented and optimized. The pre-mordanting process was also used with 5 different biomordants, at the end the washing and friction fastness properties of the fabrics dyed with and without the pre-mordanting process were observed.

Results and Discussion: The results showed that the best dye removal temperature was 70°C and the optimized dyeing conditions were a temperature of 50°C, time of 60 minutes and pH 3. In general, the use of biommordants did not promote improvements in solidity interactions.

Research Implications: The use of natural dye made from Butterfly Pea flowers in dyeing provides a blue color to the cotton fabric, which is difficult to achieve.

Originality/Value: The use of natural dye made from Butterfly Pea flowers in dyeing provides a blue color to the cotton fabric, which is difficult to achieve.
Determination of Composition and Density of Sandwich Composites Produced by Vacuum Lamination
Authors: Velasco David Coverdale Rangel , Baltazar Thiago Freitas , Viana Lena Nunes Paes , Mothe Alice Vasconcellos , Machado Ocione José , Leal Gustavo Ayres , Lopes Felipe Perisse Duarte , Vieira Carlos Mauricio Fontes 
Published: October 2024

Purpose: This study aims to characterize the composition and density of bulkheads manufactured by vacuum lamination.
Theoretical Framework: Sandwich composites are widely used due to their high specific strength, a critical factor in reducing energy consumption associated with weight, especially when manufactured through vacuum lamination. However, the final composition and density of these materials are important factors for decision-making in the project, but they are often not known beforehand.

Method: Characterization was carried out on five samples taken from different regions of the laminated plates. The composition was determined by varying the area density, and the final composite density was obtained based on the observed composition and the density of the components.

Results and conclusion: The results indicated a density of 0.21 g/cm³ for the laminated material. The analysis allowed determining the mass and volumetric fractions of the components, demonstrating the consistency of the manufacturing parameters and their adequacy to the project's requirements.

Research Implications: This study contributes to improving the vacuum lamination process by providing a methodology for characterizing the density of sandwich composites, which can be applied in various projects, as well as providing reference values.

Originality/Value: The research offers an innovative approach by exploring the variation of area density to characterize the composition of composites, contributing to the optimization of the sandwich composite manufacturing process, such as the vessel “Gigante Vermelho” used in the Desafio Solar Brasil 2022.
Household Well-Being, Environment and Health in the Peripheries of the Metropolitan Area of Oaxaca City
Authors: Reyes-Morales Rafael Gabriel , Gijón-Cruz Alicia Sylvia , Martínez Verónica Judith Yescas 
Published: October 2024

Objective: The economy of poor urban households is analyzed using a well-being function that also considers incomes, sociodemographic characteristics (CS), housing and the environment.

Theoretical structure: The dependent variable is household well-being (BF) and the explanatory variables are all incomes obtained from the market economy, as well as government transfers (TG). The CS represent resources that facilitate obtaining income; housing provides income indicators; and the environment are the physical conditions that determine the state of health.
Methodology: A probabilistic survey was applied to a sample of households in four poor peripheral neighborhoods. The equations were constructed using ordinary least squares regression and the beta coefficients measure the contribution of the variables from the four categories above to the BF. The effects of these variables on health expenditure (GS) are also measured.

Discoveries: The main incomes that contribute to the BF were determined and these make a contribution equivalent to that of the set of variables of the CS, housing and family businesses. Likewise, the environment and diseases increase GS.

Practical and social implications: Research shows that poor peripheral urban households participate in the market economy and that an increase in average age contracts their level of BF.

Originality/value: The tested model turned out to be versatile and accurate in analyzing the economies of poor urban households and has greater explanatory power than the agricultural household models and the applied general equilibrium model.
From Thesis to Scientific Articles: Publication of Neonatal Research in Ecuador
Authors: Toapanta-Pinta Paola , Oliva-Velasco Veronica , Gavilanes-Vallejo Bianca , Caicedo-Gallardo Daniela , Morales Santiago Vasco 
Published: October 2024

Objective: To determine the proportion of scientific articles published by health professionals in Neonatology services in Ecuador in the last 20 years.

Theoretical framework: Perinatal research is a priority in public health. Writing a thesis allows students to acquire research skills. Publishing these findings in indexed journals ensures their validity and relevance, becoming references for future studies, systematic reviews, hospital protocols and clinical guidelines.

Method: Bibliometric study that examined the temporal evolution, research design, language, affiliations, keywords, journals and academic degrees of thesis writers. The search was carried out in databases and repositories from the last 20 years, using Zotero, Mendeley, Rayyan and R for the analysis.

Results and Discussion: It was identified that 13% of the theses were published in indexed journals in Ecuador. (79%) corresponded to postgraduate studies in pediatrics and neonatology. Almost half (48.97%) were published in local journals and the majority (63.69%) in Spanish. The low rate of scientific publication is a common problem in Spanish-speaking countries. Ecuador contributes only 0.11% of world publications.

Research implications: Strengthen research training for students and encourage other professionals involved in newborn care to publish their theses.

Originality/Value: The publication of theses as scientific articles contributes to the advancement of knowledge. This process broadens the scope of research, enhancing its impact and application in clinical practice and evidence-based decision-making.
ЭКСПЛУАТАЦИОННАЯ РАЗВЕДКА КАК ИНСТРУМЕНТ ПОВЫШЕНИЯ УРОВНЯ ПРОМЫШЛЕННОЙ БЕЗОПАСНОСТИ ДОБЫЧИ УГЛЯ
Authors: Шаклеин С.В. , Рогова Т.Б. 
Published: October 2024

Рассмотрено значение опережающего геологического изучения недр для снижения риска возникновения аварий и инцидентов, связанных с влиянием природных геологических факторов. Предлагается конкретизировать содержание подразделов проектной документации «Геологическая изученность» и «Эксплуатационная разведка» на основании анализа результатов применения количественных методов разведанности угольных месторождений. Ответственность недропользователя за игнорирование государственных требований по изучению недр должна учитываться при расследовании причин аварий и инцидентов. Отмечается, что эксплуатационную разведку следует рассматривать как превентивное мероприятие, направленное на решение задачи повышения уровня промышленной безопасности угледобычи.The advanced exploration of mineral resources is considered to reduce the risk of accident initiation and incidents connected with natural geological factors impact. It is proposed to specify the contents of the subsections of the design documentation «State of Geological Exploration» and «Mining Exploration» based on the analysis of the results obtained during the application of the coal deposits exploration quantitative method. The responsibility of minerals developers for ignoring the state requirements for mineral resources exploration should be taken into account when investigating the causes of accidents and incidents. The mining exploration should be considered as preventive measures aimed to increase the industrial safety level during coal mining.