bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential lipid signaling from CD4+ and CD8+ T cells contributes to type 1 diabetes development
Authors: White Tayleur D. , Almutairi Abdulaziz , Gai-Tusing Ying , Stephenson Daniel J. , Stephenson Benjamin D. , Chalfant Charles E. , Lei Xiaoyong , Lu Brian , Hammock Bruce D. , DiLorenzo Teresa P. , Ramanadham Sasanka 
Published: September 2024

IntroductionWe reported that Ca2+-independent phospholipase A2β (iPLA2β)–derived lipids (iDLs) contribute to type 1 diabetes (T1D) onset. As CD4+ and CD8+ T cells are critical in promoting β-cell death, we tested the hypothesis that iDL signaling from these cells participates in T1D development. MethodsCD4+ and CD8+ T cells from wild-type non-obese diabetic (NOD) and NOD.iPLA2β+/- (NOD.HET) mice were administered in different combinations to immunodeficient NOD.scid. ResultsIn mice receiving only NOD T cells, T1D onset was rapid (5 weeks), incidence 100% by 20 weeks, and islets absent. In contrast, onset was delayed 1 week and incidence reduced 40%–50% in mice receiving combinations that included NOD.HET T cells. Consistently, islets from these non-diabetic mice were devoid of infiltrate and contained insulin-positive β-cells. Reduced iPLA2β led to decreased production of proinflammatory lipids from CD4+ T cells including prostaglandins and dihydroxyeicosatrienoic acids (DHETs), products of soluble epoxide hydrolase (sEH), and inhibition of their signaling decreased (by 82%) IFNγ+CD4+ cells abundance. However, only DHETs production was reduced from CD8+ T cells and was accompanied by decreases in sEH and granzyme B. DiscussionThese findings suggest that differential select iDL signaling in CD4+ and CD8+ T cells contributes to T1D development, and that therapeutics targeting such signaling might be considered to counter T1D.
Identification of telomere-related lncRNAs and immunological analysis in ovarian cancer
Authors: Xu Weina , Sang Shuliu , Wang Jun , Guo Shanshan , Zhang Xiao , Zhou Hailun , Chen Yijia 
Published: September 2024

BackgroundOvarian cancer (OC) is a global malignancy characterized by metastatic invasiveness and recurrence. Long non-coding RNAs (lncRNAs) and Telomeres are closely connected with several cancers, but their potential as practical prognostic markers in OC is less well-defined.MethodsRelevant mRNA and clinical data for OC were sourced from The Cancer Genome Atlas (TCGA) database. The telomere-related lncRNAs (TRLs) prognostic model was established by univariate/LASSO/multivariate regression analyses. The effectiveness of the TRLs model was evaluated and measured via the nomogram. Additionally, immune infiltration, tumor mutational load (TMB), and drug sensitivity were evaluated. We validated the expression levels of prognostic genes. Subsequently, PTPRD-AS1 knockdown was utilized to perform the CCK8 assay, colony formation assay, transwell assay, and wound healing assay of CAOV3 cells.ResultsA six-TRLs prognostic model (PTPRD-AS1, SPAG5-AS1, CHRM3-AS2, AC074286.1, FAM27E3, and AC018647.3) was established, which can effectively predict patient survival rates and was successfully validated using external datasets. According to the nomogram, the model could effectively predict prognosis. Furthermore, we detected the levels of regulatory T cells and M2 macrophages were comparatively higher in the high-risk TRLs group, but the levels of activated CD8 T cells and monocytes were the opposite. Finally, the low-risk group was more sensitive to anti-cancer drugs. The mRNA levels of PTPRD-AS1, SPAG5-AS1, FAM27E3, and AC018647.3 were significantly over-expressed in OC cell lines (SKOV3, A2780, CAOV3) in comparison to normal IOSE-80 cells. AC074286.1 were over-expressed in A2780 and CAOV3 cells and CHRM3-AS2 only in A2780 cells. PTPRD-AS1 knockdown decreased the proliferation, cloning, and migration of CAOV3 cells.ConclusionOur study identified potential biomarkers for the six-TRLs model related to the prognosis of OC.
Host heterogeneity in humoral bactericidal activity can be complement independent
Authors: Abe Ryuichiro , Ram-Mohan Nikhil , Zudock Elizabeth Jordan , Lewis Shawna , Carroll Karen C. , Yang Samuel 
Published: September 2024

BackgroundHumoral bactericidal activity was first recognized nearly a century ago. However, the extent of inter-individual heterogeneity and the mechanisms underlying such heterogeneity beyond antibody or complement systems have not been well studied.MethodsThe plasma bactericidal activity of five healthy volunteers were tested against 30 strains of Gram-negative uropathogens, Klebsiella pneumoniae and Escherichia coli, associated with bloodstream infections. IgG and IgM titers specific to K. pneumoniae strains KP13883 and KPB1 were measured by ELISA, and complement inhibitor was used to measure the contribution of complement-induced killing. Furthermore, MALDI-TOF mass spectrometry was conducted to determine the metabolomic components of plasma with bactericidal properties in 25 healthy individuals using Bayesian inference of Pearson correlation between peak intensity and colony counts of surviving bacteria.ResultsPlasma bactericidal activity varied widely between individuals against various bacterial strains. While individual plasma with higher IgM titers specific to K. pneumoniae strain KP13883 showed more efficient killing of the strain, both IgM and IgG titers for K. pneumoniae strain KPB1 did not correlate well with the killing activity. Complement inhibition assays elucidated that the complement-mediated killing was not responsible for the inter-individual heterogeneity in either isolate. Subsequently, using MALDI-TOF mass spectrometry on plasmas of 25 healthy individuals, we identified several small molecules including gangliosides, pediocins, or saponins as candidates that showed negative correlation between peak intensities and colony forming units of the test bacteria.ConclusionThis is the first study to demonstrate the inter-individual heterogeneity of constitutive innate humoral bactericidal function quantitatively and that the heterogeneity can be independent of antibody or the complement system.
Mid-infrared optical coherence tomography and machine learning for inspection of 3D-printed ceramics at the micron scale
Authors: Heise Bettina , Zorin Ivan , Duswald Kristina , Karl Verena , Brouczek Dominik , Eichelseder Julia , Schwentenwein Martin 
Published: September 2024

IntroductionIn this paper, recent developments in non-destructive testing of 3D-printed ceramics and monitoring of additive manufacturing of ceramics are presented.MethodsIn particular, we present the design and use of an inline mid-infrared optical coherence tomography (MIR-OCT) system to evaluate printed and micro-structured specimens in lithography-based ceramic manufacturing (LCM).ResultsThe proposed system helps with the detection of microdefects (e.g., voids, inclusions, deformations) that are already present in green ceramic components, thereby reducing the energy and costs incurred.DiscussionThe challenges during integration are discussed. Especially, the prospects for MIR-OCT imaging combined with machine learning are illustrated with regard to inline inspection during LCM of printed ceramics.
Causal relationship between non-alcoholic fatty liver disease and sarcopenia: a bidirectional Mendelian randomization study
Authors: Chen Meng , Liu Jili , Xia Xin , Wang Yarong , Zheng Hongying 
Published: September 2024

IntroductionA correlation between non-alcoholic fatty liver disease and sarcopenia is demonstrated, but the causality remains unclear. Our study aims to clarify the point of genetics between non-alcoholic fatty liver disease (NAFLD) and sarcopenia at the level of gene prediction through two-sample Mendelian randomization (MR) analysis.MethodsThe study employed the two-sample MR approach to investigate the bi-directional causality between NAFLD and sarcopenia. Published summary statistics were used to obtain instrumental variables (IVs) at the genome-wide significance level.ResultsIVW analysis showed that the risk of NAFLD was reduced when walking pace was increased (OR = 0.435, 95%CI 0.240–0.789, p = 0.006); Increasing appendicular lean mass (ALM) decreased the risk of NAFLD (OR = 0.906, 95%CI 0.838–0.980, p = 0.014); Those older than 60 were more likely to suffer from NAFLD if they had low grip strength (OR = 1.411, 95%CI 1.087–1.830, p = 0.0012). In the reverse MR study, weight median analysis showed that NAFLD caused a decrease in ALM (OR = 0.953, 95%CI 0.957–0.994, p = 0.001); whereas NAFLD showed no correlation with usual walking pace or grip strength (all with p &gt; 0.05). MR-Egger regression analysis showed that there was no horizontal pleiotropy in the SNPs (all with p &gt; 0.05).ConclusionThe characteristics related to sarcopenia (usual walking pace, appendicular lean mass and low hand grip strength) may play a causal role in the development of nonalcoholic fatty liver disease, although the underlying mechanisms need to be further investigated. The presence of specific single nucleotide polymorphisms (SNPs) such as rs3747207, rs429358, and rs73001065 has been identified in the PNPLA3, APOE, and MAU2 proteins. These genetic markers represent potential targets for future interventions aimed at addressing, managing, or mitigating the risk of NAFLD.
Application of autologous platelet-rich gel formed by calcium gluconate combined with hormone therapy for endometrial repair after hysteroscopic transcervical resection of adhesion surgery and successful pregnancy: case report and literature review
Authors: Li Yunying , Han Yingxue , Su Xiaojuan , Cao Junjuan , Liu Junxia , Zhang Wenjuan 
Published: September 2024

IntroductionIntrauterine adhesion (IUA), a common gynecological disease, is mainly caused by traumatic or infectious factors that lead to basal endometrial layer physiological repair disorders. IUA is mostly treated via hysteroscopic transcervical resection of adhesion and although it can restore uterine cavity shape, its endometrial repair effectiveness is limited. The figures showed that after surgery, patients with IUA have a high recurrence rate. Therefore, quick endometrial damage repair is key to successful treatment.Case presentationA 34-year-old patient visited our hospital after experiencing amenorrhea for 4 months following an induced abortion and had a fertility requirement. Based on the American Fertility Society intrauterine scores, the patient was diagnosed with moderate IUA. She underwent transcervical resection of adhesion, followed by autologous platelet-rich gel intrauterine perfusion and periodic estrogen–progesterone treatment for three menstrual cycles. No complications developed during treatment and the patient’s endometrium was significantly repaired, with successful pregnancy being achieved.ConclusionAutologous platelet-rich gel promoted endometrial repair and acted as a mechanical barrier to prevent intrauterine adhesion. This approach May offer new insights into IUA treatment.
Comparative bibliometric analysis of artificial intelligence-assisted polyp diagnosis and AI-assisted digestive endoscopy: trends and growth in AI gastroenterology (2003–2023)
Authors: Peng Ziye , Wang Xiangyu , Li Jiaxin , Sun Jiayi , Wang Yuwei , Li Yanru , Li Wen , Zhang Shuyi , Wang Ximo , Pei Zhengcun 
Published: September 2024

IntroductionArtificial intelligence is already widely utilized in gastroenterology. This study aims to comprehensively evaluate the research hotspots and development trends within the field of AI in gastroenterology by employing bibliometric techniques to scrutinize geographical distribution, authorship, affiliated institutions, keyword usage, references, and other pertinent data contained within relevant publications.MethodsThis investigation compiled all pertinent publications related to artificial intelligence in the context of gastrointestinal polyps and digestive endoscopy from 2003 to 2023 within the Web of Science Core Collection database. Furthermore, the study harnessed the tools CiteSpace, VOSviewer, GraphPad Prism and Scimago Graphica for visual data analysis. The study retrieved a total of 2,394 documents in the field of AI in digestive endoscopy and 628 documents specifically related to AI in digestive tract polyps.ResultsThe United States and China are the primary contributors to research in both fields. Since 2019, studies on AI for digestive tract polyps have constituted approximately 25% of the total AI digestive endoscopy studies annually. Six of the top 10 most-cited studies in AI digestive endoscopy also rank among the top 10 most-cited studies in AI for gastrointestinal polyps. Additionally, the number of studies on AI-assisted polyp segmentation is growing the fastest, with significant increases in AI-assisted polyp diagnosis and real-time systems beginning after 2020.DiscussionThe application of AI in gastroenterology has garnered increasing attention. As theoretical advancements in AI for gastroenterology have progressed, real-time diagnosis and detection of gastrointestinal diseases have become feasible in recent years, highlighting the promising potential of AI in this field.
Association between ABO blood groups and hematological myeloid neoplasms in adolescents and adults
Authors: Zhou Miao , Li Tongyu , Sun Yongcheng , Ouyang Guifang , Zhuang Wanchuan , Zhang Ping 
Published: September 2024

BackgroundPrior research suggests a potential link between ABO blood types and susceptibility to various malignancies. The correlation between ABO blood types and hematological myeloid neoplasms, however, remains inadequately explored.ObjectiveThis study investigates the association between ABO blood groups and the incidence of hematological myeloid neoplasms in adolescents and adults.MethodsIn this retrospective clinical study, 1,022 adolescent and adult cases of myeloid neoplasms diagnosed at our institution were initially considered. After excluding conditions potentially linked to ABO blood types from prior studies, 792 eligible cases were analyzed. These cases were categorized based on disease subtypes and compared with a control group for blood type distribution.ResultsOur findings reveal a significantly higher prevalence of blood type A in patients with myeloid neoplasms compared to the control group, except for chronic myelocytic leukemia and myeloproliferative neoplasms. Conversely, the prevalence of blood type AB in myeloid neoplasms was notably lower than in the control group.ConclusionThe study suggests a potential association between ABO blood types and the risk of developing hematological myeloid neoplasms in adolescents and adults. Further research is warranted to elucidate the underlying mechanisms of this relationship.
Association between systemic immune-inflammation index and cataract among outpatient US adults
Authors: Huang Jin , Wu Hongjiang , Yu Fang , Wu Fangkun , Hang Chen , Zhang Xiaoya , Hao Yiting , Fu Hao , Xu Hongting , Li Rong , Chen Ding 
Published: September 2024

BackgroundWhile several studies have noted a higher SII correlates with multiple diseases, research on the association between SII and cataract remains limited. Our cross-sectional study seeks to examine the association between SII and cataract among outpatient US adults.MethodsThis compensatory cross-sectional study utilized NHANES data from 1999 to 2008 cycles, conducting sample-weighted multivariate logistic regression and stratified analysis of subgroups.ResultsAmong 11,205 adults included in this study (5,571 [46.2%] male; 5,634 [53.8%] female), 2,131 (15.2%) had cataract and 9,074 (84.8%) did not have cataract. A fully adjusted model showed that SII higher than 500 × 109/L was positively correlated with an increased risk of cataracts among women (OR, 1.27; 95% CI, 1.02–1.59) (p = 0.036). However, no difference was found in the men subgroup, and there was no significant interaction between SII and sex.ConclusionOur results indicated that a SII higher than 500 × 109/L was positively correlated with an increased risk of cataracts in women. This study is the first to specifically investigate the impact of a high SII on cataract risk in outpatient adults in the United States. By effectively addressing inflammation, it is possible to mitigate cataract progression and significantly enhance patient outcomes.
Combination of in silico and molecular techniques for discrimination and virulence characterization of marine Brucella ceti and Brucella pinnipedialis
Authors: Girault Guillaume , Freddi Luca , Jay Maryne , Perrot Ludivine , Dremeau Alexandre , Drapeau Antoine , Delannoy Sabine , Fach Patrick , Ferreira Vicente Acacia , Mick Virginie , Ponsart Claire , Djokic Vitomir 
Published: September 2024

IntroductionMammals are the main hosts for Brucella sp., agents of worldwide zoonosis. Marine cetaceans and pinnipeds can be infected by Brucella ceti and B. pinnipedialis, respectively. Besides classical bacteriological typing, molecular approaches such as MLVA, MLSA, and whole-genome sequencing (WGS) can differentiate these species but are cumbersome to perform.MethodsWe compared the DNA and genome sequences of 12 strains isolated from nine marine mammals, with highly zoonotic B. melitensis, B. abortus, and B. suis, and the publicly available genomes of B. ceti and B. pinnipedialis. In silico pipelines were used to detect the antimicrobial resistance (AMR), plasmid, and virulence genes (VGs) by screening six open-source and one home-made library.Results and discussionOur results show that easier-to-use HRM-PCR, Bruce-ladder, and Suis-ladder can separate marine Brucella sp., and the results are fully concordant with other molecular methods, such as WGS. However, the restriction fragment length polymorphism (RFLP) method cannot discriminate between B. pinnipedialis and B. ceti B1-94-like isolates. MLVA-16 results divided the investigated strains into three clades according to their preferred host, which was confirmed in WGS. In silico analysis did not find any AMR and plasmid genes, suggesting antimicrobial susceptibility of marine Brucella, while the presence of the VGs btpA gene was variable dependent on the clade.ConclusionThe HRM-PCR and Suis-ladder are quick, easy, and cost-effective methods to identify marine Brucella sp. Moreover, in silico genome analyses can give useful insights into the genetic virulence and pathogenicity potential of marine Brucella strains.
Impact of weight variation on the microbiome of yak dams and calves
Authors: Wang Hongzhuang , Basang Wangdui , Pingcuo Zhandui , Jiang Nan , Sun Guangming , Nawaz Shah , Cidan Yangji , Liu Yang , Zhu Yanbin , Luosang Dunzhu 
Published: September 2024

IntroductionLimited information exists regarding the microbiome composition of yak calves of varying weights. Therefore, this study aimed to investigate the microbiomes of mother-calf pairs with different weight profiles.MethodsFecal and blood samples were collected from both lower-weight (CB) and higher-weight (HB) yak calves, along with their corresponding female yaks (CA, HA).ResultsThe results revealed significantly higher levels of T-AOC (total antioxidant capacity) and GSH-Px (glutathione peroxidase) in HB animals (p &lt; 0.001). Sequencing yielded 652,181 and 643,369 filtered reads in female and calf yaks, respectively. Alpha diversity analysis indicated that Chao1, Faith_pd, and Observed species were significantly higher in CA compared to HA (p &lt; 0.01). Furthermore, nine genera were notably different between HA and CA yaks, including Avispirillum, Fimenecus, CAG-1031, Odoribacter 865974, and Jeotgalicoccus A 310962. Compared to CB yaks, CA animals exhibited significant differences in one phylum and six genera, including CAG-485 (p &lt; 0.05), CAG-83 (p &lt; 0.01), Copromorpha (p &lt; 0.01), Phocaeicola A 858004 (p &lt; 0.05), and UBA2253 (p &lt; 0.05).ConclusionIn summary, higher-weight yak calves demonstrated increased oxidative resistance, and weight profiles were linked to the microbiomes of both female yaks and their calves. These findings offer valuable insights for optimizing yak breeding practices in high-altitude regions.
Combining early lower eyelid surgery with neuromuscular retraining for synkinesis prevention after facial palsy: the role of the eye in aberrant facial nerve regeneration
Authors: Di Stadio Arianna , Ralli Massimo , De Luca Pietro , Sossamon Jake , Frohman Teresa C. , Altieri Marta , La Mantia Ignazio , Ferlito Salvatore , Frohman Elliot M. , Brenner Michael J. 
Published: September 2024

BackgroundFacial synkinesis (FS) is a distressing sequela of facial palsy (FP) characterized by involuntary, simultaneous movements of facial muscles occurring during voluntary facial expressions. Treatment of synkinesis is challenging, and preventive methods are needed.AimThis study evaluated the efficacy of physical facial nerve rehabilitation (PFNR) therapy alone vs. PNFR with eyelid surgery to correct lagophthalmos and prevent the onset of synkinesis.MethodsTwenty five outpatients were randomized to receive either PFNR alone (neuromuscular retraining and Kabat proprioceptive neuromuscular facilitation) or PNFR and early (90 days after FP onset) eyelid surgery (involving a conservative oculoplastic correction for lagophthalmos with epiphora or ectropion). Comprehensive otolaryngological assessments and Magnetic Resonance Imaging (MRI) were conducted. Synkinesis progression was measured using Another Disease Scale (ADS) at baseline, 3-, 6-, 12-, and 24-months post-treatment. The data were analyzed with ANOVA, τ-test, Chi-Square analyses.ResultsPatients undergoing eyelid surgery with PFNR showed faster (p &lt; 0.001) and better recovery of facial movements (p &lt; 0.05) than patients receiving PFNR alone comparing T0 and T12 (p &lt; 0.0001). No synkinesis were observed in the PFNR plus surgery group while 37% of patients in PFNR alone had synkinesis (p = 0.03). At 24 months, none of the patients in the surgery group presented synkinesis.ConclusionCombining early surgical treatment of paralytic lagophthalmos or epiphora with PFNR accelerated functional recovery and reduced synkinesis in patients with FP compared to facial rehabilitation alone. Further investigations in larger populations with long-term follow-up are needed.Clinical trial registrationhttps://clinicaltrials.gov/study/NCT06538103, NCT06538103.
Theta burst stimulation on the fronto-cerebellar connective network promotes cognitive processing speed in the simple cognitive task
Authors: Zhao Ning , Tao Jing , Wong Clive , Wu Jing-song , Liu Jiao , Chen Li-dian , Lee Tatia M. C. , Xu Yanwen , Chan Chetwyn C. H. 
Published: September 2024

BackgroundThe fronto-cerebellar functional network has been proposed to subserve cognitive processing speed. This study aims to elucidate how the long-range frontal-to-cerebellar effective connectivity contributes to faster speed.MethodsIn total, 60 healthy participants were randomly allocated to three five-daily sessions of transcranial magnetic stimulation conditions, namely intermittent theta-burst stimulation (iTBS, excitatory), continuous theta-burst stimulation (CTBS, inhibitory), or a sham condition. The sites of the stimulations were the right pre-supplementary motor area (RpSMA), medial cerebellar vermis VI (MCV6), and vertex, respectively. Performances in two reaction time tasks were recorded at different time points.ResultsPost-stimulation speeds revealed marginal decreases in the simple but not complex task. Nevertheless, participants in the excitatory RpSMA and inhibitory MCV6 conditions showed direct and negative path effects on faster speeds compared to the sham condition in the simple reaction time (SRT) task (β = −0.320, p = 0.045 and β = −0.414, p = 0.007, respectively). These path effects were not observed in the SDMT task.DiscussionRpSMA and MCV6 were involved in promoting the path effects of faster reaction times on simple cognitive task. This study offers further evidence to support their roles within the long-range frontal-to-cerebellar connectivity subserving cognitive processing speed. The enhancement effects, however, are likely limited to simple rather than complex mental operations.