bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential response of roots growth of soybean germplasm under low pH and manganese toxicity
Authors: KUSWANTORO HERU 
Published: January 2017

Abstract. Kuswantoro H. 2016. Differential response of roots growth of soybean germplasm under low pH and manganese toxicity. Biodiversitas 18: 257-262. Most of Indonesia dry land are covered by acid soil. The growth and development of the plant in this acid soil cope with low pH and micronutrients toxicity. The objective of this research was to study the response of root growth of soybean germplams to low pH and manganese toxicity. Nine soybean germplasm were treated in aquadest with pH 7 as control, aquadest with pH 4, and 75 ppm Mn with pH 4 in Seed Laboratory of Indonesian Legume and Tuber Crops Research Institute, Malang, Indonesia. Results showed that root length, number of lateral roots, root dry weight were lower in low pH and Mn toxicity than in the control. Shoot dry weight, hypocotyl length and epicotyl length were not influenced by low pH and Mn toxicity. Some genotypes showed increasing root length in low pH, and increasing number of lateral roots and root dry weight in Mn toxicity. Based on the acid soil adaptation index (ASAI) on root length, MLGG 0493 and MLGG 0496 showed the highest value index in three comparative conditions. The genotype of MLGG 0496 also showed the highest ASAI value on numbers of lateral root in three comparative conditions. MLGG 0494 achieved the highest ASAI value of root dry weight. However, the use of root dry weight as a criterion in soybean adaptation in low pH and Mn toxicity should be studied further because the tolerance is not just in increasing root dry weight as a result of root thickening.
Keywords: ASAI, germplasm, low pH, Mn toxicity, root growth, soybean
Paper-Based MicroRNA Expression Profiling from Plasma and Circulating Tumor Cells
Authors: Leong Sai Mun , Tan Karen Mei-Ling , Chua Hui Wen , Huang Mo-Chao , Cheong Wai Chye , Li Mo-Huang , Tucker Steven , Koay Evelyn Siew-Chuan 
Published: January 2017

Abstract

BACKGROUND
Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging.

METHODS
Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood. Specifically, we devised a workflow involving the use of Flinders Technology Associates (FTA)® Elute Card with a digital PCR-inspired “partitioning” method to extract and purify miRNAs from plasma and CTCs.

RESULTS
We demonstrated the sensitivity of this method to detect miRNA expression from as few as 3 cancer cells spiked into human blood. Using this method, background miRNA expression was excluded from contaminating blood cells, and CTC-specific miRNA expression profiles were derived from breast and colorectal cancer patients. Plasma separated out during purification of CTCs could likewise be processed using the same paper-based method for miRNA detection, thereby maximizing the amount of patient-specific information that can be derived from a single blood draw.

CONCLUSIONS
Overall, this paper-based extraction method enables an efficient, cost-effective workflow for maximized recovery of small RNAs from limited biological samples for downstream molecular analyses.
Apakah Hipertensi Arteri Pulmonal Merupakan Faktor Risiko Malnutrisi pada Penyakit Jantung Bawaan Asianotik dengan Pirau Kiri ke Kanan
Authors: Handiarsa Arief , Nugroho Sasmito , Prawirohartono Endy Paryanto Prawirohartono 
Published: January 2017

Latar belakang. Penyakit jantung bawaan (PJB) asianotik pirau kiri ke kanan menyebabkan terjadinya hipertensi arteri pulmonal (HAP). Malnutrisi merupakan komplikasi penting berhubungan dengan PJB, kejadiannya semakin meningkat bila disertai HAP, PJB sianotik, dan gagal jantung kongestif.Tujuan.Mengetahui HAP sebagai faktor risiko malnutrisi pada anak dengan PJB asianotik pirau kiri ke kanan.Metode. Penelitian dengan design kasus-kontrol. Subjek penelitian adalah anak berusia 6 bulan – 18 tahun dengan PJB asianotik pirau kiri ke kanan yang berobat ke Poliklinik Kardiologi Anak RSUP Dr. Sardjito Yogyakarta periode Juni-Juli 2013 yang memenuhi kriteria inklusi. Sampel diambil secara konsekutif sampling, setelah didapatkan kelompok kasus (anak yang mengalami malnutrisi) dan kontrol (anak yang tidak mengalami malnutrisi), kemudian diidentifikasi adanya faktor risiko HAP berdasarkan data rekam medis. Analisis univariat untuk menghitung odds ratio (OR) dan interval kepercayaan 95% (IK95%) serta analisis multivariat dengan regresi logistik.Hasil. Terdapat 76 pasien diikutsertakan, sampel kelompok kasus 38 (50%), kelompok kontrol 38 (50%) anak. Analisis multivariat menunjukkan HAP tidak memiliki hubungan kuat sebagai faktor risiko terjadinya malnutrisi pada anak dengan PJB asianotik pirau kiri ke kanan dengan nilai p=0,006, OR 0,100 (IK95%: 0,020-0,512). Lama terapi >36 bulan pada PJB asianotik pirau kiri ke kanan meningkatkan risiko kejadian malnutrisi secara bermakna dibandingkan lama terapi <12 bulan dengan nilai p=0,035, OR 4,095(IK95%: 1,107-15,155).Kesimpulan. Hipertensi arteri pulmonal bukan merupakan faktor risiko terjadinya malnutrisi pada PJB asianotik pirau kiri ke kanan tetapi merupakan faktor protektif.
Kadar Interferon Gamma Kultur Sel Limfosit pada Anak yang Mendapat Vaksinasi BCG
Authors: Fitria Liza , Putra Andani Eka , Yani Finny Fitry , Basir Darfioes 
Published: January 2017

Latar belakang. Vaksinasi BCG mempunyai efek proteksi yang bervariasi, menurun dengan bertambahnya umur anak. Perkembangan pengetahuan di bidang biologi molekuler telah mengembangkan pemeriksaan IFN-γ untuk mendeteksi respon vaksinasi.Tujuan. Mengetahui lamanya efek proteksi BCG dan hubungan kadar IFN-γ dengan umur, status gizi, umur mendapatkan vaksinasi BCG dan sarana kesehatan tempat vaksinasi.Metode. Suatu studi cross sectional, stratified random sampling, pada anak yang telah mendapat vaksinasi BCG. Kadar IFN-γ diukur dari sel limfosit yang telah dikultur. Analisis statistik menggunakan uji Anova dan Independent sample t tes.Hasil. Kadar IFN-γ tertinggi pada kelompok umur 4-11 bulan. Terdapat perbedaan bermakna kadar IFN-γ antara kelompok umur 4-11 bulan dengan 1-4 tahun dan 1-4 tahun dengan 5-9 tahun. Tidak terdapat perbedaan bermakna antara status gizi, umur mendapatkan vaksinasi BCG, sarana kesehatan tempat vaksinasi BCG dengan kadar IFN-γ.Kesimpulan. Kadar IFN-γ yang tinggi menunjukkan vaksinasi BCG efektif pada anak, tetapi efektifitasnya menurun seiring dengan pertambahan umur. Sari
Hubungan antara Kortisol Saliva dan Masalah Mental Emosional pada Anak Usia 3–5 Tahun
Authors: Whisnubrata Tommy Nugrahadi , Fadlyana Eddy , Rahayuningsih Sri Endah 
Published: January 2017

Latar belakang. Masalah mental emosional pada anak usia prasekolah harus dideteksi dan diantisipasi sedini mungkin. Masalah mental emosional memengaruhi mekanisme aksis hipotalamus-pituitari-adrenal, menghasilkan produk akhir kortisol.Tujuan. Menentukan hubungan kadar kortisol saliva dan masalah mental emosional.Metode. Penelitian potong lintang ini dilakukan pada bulan Desember 2015 - Januari 2016 terhadap 82 anak usia 3–5 tahun yang dititipkan di tempat penitipan anak di Kota Bandung. Orangtua diminta untuk mengisi kuesioner strength and difficulties questionnaire (SDQ) untuk skrining masalah mental emosional subjek. Kortisol saliva subjek penelitian dianalisis menggunakan salivary cortisol kit dari Salimetrics®. Analisis data untuk menentukan hubungan antara kortisol saliva dan masalah mental emosional dilakukanmenggunakan analisis regresi logistik.Hasil. Terdapat hubungan bermakna antara kadar kortisol saliva dan masalah mental emosional (p=0,027; OR=3,431). Terdapat hubungan bermakna antara kadar kortisol abnormal dengan variabel conduct (p=0,001) dan emosi (p=0,017).Kesimpulan. Kadar kortisol saliva berhubungan dengan masalah mental emosional pada anak usia 3-5 tahun. Kadar kortisol berhubungan dengan variabel conduct dan emosi.
Korelasi Skala FLACC dengan Kadar Alfa Amilase Saliva pada Anak Palsi Serebral
Authors: Sabur Rissa Puspitari , Risan Nelly Amalia , Chairulfatah Alex 
Published: January 2017

Latar belakang. Skala face, leg, activities, cry, and consolability (FLACC) diketahui dapat menilai nyeri pada anak palsi serebral (PS). Alfa amilase saliva (AAS) yang diambil dari saliva juga meningkat pada anak palsi serebral yang dilakukan tindakan invasif pengambilan darah vena. Korelasi antara kedua jenis pemeriksaan ini belum diketahui.Tujuan. Menentukan korelasi skor FLACC dengan kadar AAS pada anak PS.Metode. Penelitian analitik observasional dengan rancangan potong lintang, yaitu mengukur derajat nyeri menggunakan skala FLACC dengan mengamati perilaku subjek dan menilai perilaku sesuai yang tertera pada formulir FLACC. Kadar AAS diperiksa sebelum dilakukan tindakan fisioterapi. Pengukuran kadar AAS dengan metode spektrofotometri pada anak yang telah didiagnosis PS yang datang berobat di Poliklinik Neurologi Anak dan Unit Rehabilitasi Medik Rumah Sakit Hasan Sadikin Bandung, berusia 6 bulan–14 tahun, periode Januari - Maret 2016. Analisis data digunakan uji korelasi Rank Spearman dengan kemaknaan p<0,05.Hasil. Didapat 42 pasien anak dengan diagnosis PS. Uji statistik menggunakan uji korelasi Rank Spearman menunjukkan terdapat korelasi yang lemah antara skor FLACC dan kadar AAS (r=0,364; p=0,034) pada kelompok usia >24 bulan.Kesimpulan. Peningkatan skor FLACC kecenderungan diikuti dengan peningkatan kadar AAS pada anak PS.
VARIAÇÃO DA CONCENTRAÇÃO DE BACTÉRIAS INDICADORAS DE CONTAMINAÇÃO FECAL NAS DIVERSAS FASES DO PROCESSO DE TRATAMENTO DE ÁGUA DE ABASTECIMENTO PÚBLICO DA CIDADE DE ARARAQUARA - SÃO PAULO
Authors: Alcântara Ilton Luiz , Simões Maria Jacira Silva , Farache Filho Adalberto 
Published: January 2017

O abastecimento de água de qualidade e em quantidade suficiente é de fundamental importância para a saúde da população. A potabilidade bacteriológica é determinada principalmente pela verificação da presença de bactérias do grupo coliforme (totais e fecais) indicadoras de poluição fecal, maior responsável pela transmissão de doenças infecciosas à população. O objetivo desta pesquisa foi verificar a eficácia dos processos de tratamento executados nas Estações de Tratamento de Água (ETA) de Araraquara, determinando as concentrações de bactérias coliformes totais e fecais, em
cada etapa do processo de tratamento: água bruta, após floculação/decantação, filtração e cloração - relacionadas com a turbidez, avaliando se a água produzida está de acordo com os padrões do Ministério da Saúde e do Estado de São Paulo. O número mais provável (NMP/100mL) de bactérias coliformes totais e coliformes fecais foi determinado pela técnica das fermentações em tubos múltiplos, provas presuntiva e confirmatória. As análises foram realizadas mensalmente por um período de doze meses. Em todas as
colheitas verificou-se que a água bruta apresentou grande quantidade de coliformes (NMP/100mL), daí a necessidade de tratamento e a importância fundamental que a fase de desinfecção tem para que a água chegue a população dentro dos padrões de qualidade. Concluíu-se que após a cloração ocorreu redução no número (NMP/100mL) de bactérias
coliformes totais e fecais paralela à redução de turbidez e que a água produzida pelas ETAs atende aos padrões determinados pelo Ministério da Saúde e do Estado de São
Paulo.
ASPECTOS POPULACIONAIS DA LAGOSTA PINTADA PANULIRUS ECHINATUS SMITH, 1869 NA RESERVA BIOLÓGICA DO ATOL DAS ROCAS – BRASIL
Authors: Silva Maurizélia De Brito , Campos Carlos Eduardo Costa , Targino Simone Godeiro , Melo Carlos Eduardo Dias da Costa Arcoverde de 
Published: January 2017

As lagostas da família Palinuridae, Panulirus argus, P. laevicauda e P. echinatus são um dos principais recursos econômicos do setor pesqueiro no Nordeste do Brasil. Dentre estas, a lagosta pintada, P. echinatus é a única das espécies capturadas comercialmente que, por não existirem dados suficientes sobre sua biologia e dinâmica, não possui regulamentação específica. Os exemplares amostrados foram capturados manualmente em piscinas recifais localizadas no platô recifal da Reserva Biológica do Atol das Rocas durante o período de julho de 2000 a março de 2001. As fêmeas
predominaram na população, representando 66,9% do total de indivíduos amostrados. A amplitude de comprimento total para fêmeas foi de 8,1 a 22,8 cm e, 5,8 a 25,7 cm para
machos.
ESPAÇOS LIVRES PÚBLICOS DA CIDADE DE ILHA SOLTEIRA, SP - BRAZIL
Authors: Freitas-Lima Elizete Aparecida Checon de , Cavalheiro Felisberto 
Published: January 2017

O presente trabalho investigou os espaços livres públicos (ELP) da cidade de Ilha Solteira, SP. Os ELPs foram identificados e classificados em várias categorias. Foi
também determinado o índice de espaços livres públicos (IELP) - relação entre o número de espaços livres públicos e o número de habitantes - para a cidade de Ilha Solteira.
Finalmente foram propostas algumas estratégias de manejo dos ELPs, no contexto do planejamento urbano.
From a Geometry of Vision to a Geometry of Light in Early-Modern Perspective
Authors: Fowler Caroline O. 
Published: January 2017

Must the architect or artist understand how the world is perceived on the convex surface of the eye to simulate the three-dimensional world on a two-dimensional plane? For many early-modern artists, optics – defined as the science of vision – was fundamental. Yet, for architects, the integration of optical theories into two-dimensional representations of buildings remained more tenuous. Architectural drawing depended on orthographic projection and the representation of built form through plan, section and elevation, which did not seek to mimic the process of vision. If anything, architectural drawing separated itself from the illusion of vision in its attempt to account for the discrepancies between the represented and the built form. Nevertheless, the shifting science of optics would come to influence the two-dimensional representation of the built world for both architects and painters.
This essay covers a broad survey of perspectival treatises from the fifteenth to the eighteenth century in order to consider how changes in the science of optics shifted the means by which artists and architects theorized the representation of space and the simulated illusion of perspective. As will be seen, the seemingly innocuously obvious geometric parts for the creation of perspectival space – the Euclidean point and line – became obsolete in the eighteenth century due to fundamental shifts in the science of optics. Whereas once optics was a study of vision through points and lines, in the seventeenth century with the works of Johannes Kepler (1571–1630) and René Descartes (1596–1650), among many others, optics transformed into a study of light. As light rather than vision became the focus of optics and its geometrical laws, the connection between a geometry of vision and a geometry of spatial representation became challenged. When light – not vision – became subject to the laws of geometry, the eye became one instrument among many (lenses, camera obscuras, microscopes and telescopes) capable of deception and fault. In turn, geometry lost its intellectual and metaphysical resonances and became a practical tool of application. The influence of the visioning technology of geometry on perspectival drawing for both the built and the figurative world lost its theoretical foundation. No longer a technology of vision, the art of geometry became reduced to non-theoretical rudimentary forms for beginning draftsmen.
Effect of Long Term Service at Elevated Temperatures on Mechanical Properties of Manaurite XM Reformer Tubes
Authors: Łabanowski J. , Jurkowski M. , Landowski M. 
Published: January 2017

Abstract
Microstructure transformations occur in the Manaurite XM cast steel tubes during long-term operation in the reformer furnace were revealed and described. The relationship between mechanical properties, an increase of internal diameter of the tube and microstructure degradation is discussed. Static tensile test was performed on two types of samples with different shapes. It has been shown differences in the results of tests and an explanation of this phenomenon.
Individual construction of freeform-fabricated polycaprolactone scaffolds for osteogenesis
Authors: Liu Lu , Shi Guixin , Cui Ying , Li Hui , Li Zhengchao , Zeng Qiangcheng , Guo Yong 
Published: January 2017

Abstract
The construction of engineered bone mostly focuses on simulating the extracellular matrix (ECM) for proper biological activity. However, the complexity of architecture and the variability of the mechanical properties of natural bones are related to individual differences in age, nutritional state, mechanical loading and disease status. Defect substitutions should be normed with the host natural bone, balancing architectural and mechanical adaption, as well as biological activity. Using a freeform fabrication (FFF) method, we prepared polycaprolactone (PCL) scaffolds with different architectures. With simulation of structural and mechanical parameters of rabbit femur cancellous bone, individual defect substitution with the characteristics of the rabbit femur was obtained with high porosity and connectivity. Biological adaption in vitro was examined and osteoid formation in vivo was assessed by implantation in situ. Simulating the femur cancellous bone, 300-μm FFF PCL scaffolds had better architectural and mechanical properties. The protocol produced an architecturally, mechanically and biologically adaptive construction of an individual model for rapid-prototype PCL scaffolds. A guide system was developed to accurately reproduce virtually individual defect substitutions of the bone.
Detecting paraprotein interference on a direct bilirubin assay by reviewing the photometric reaction data
Authors: García-González Elena , Aramendía Maite , González-Tarancón Ricardo , Romero-Sánchez Naiara , Rello Luis 
Published: January 2017

AbstractBackground:The direct bilirubin (D-Bil) assay on the AU Beckman Coulter instrumentation can be interfered by paraproteins, which may result in spurious D-Bil results. In a previous work, we took advantage of this fact to detect this interference, thus helping with the identification of patients with unsuspected monoclonal gammopathies. In this work, we investigate the possibility to detect interference based on the review of the photometric reactions, regardless of the D-Bil result.Methods:The D-Bil assay was carried out in a set of 2164 samples. It included a group of 164 samples with paraproteins (67 of which caused interference on the assay), as well as different groups of samples for which high absorbance background readings could also be expected (i.e. hemolyzed, lipemic, or icteric samples). Photometric reaction data were reviewed and receiver operating characteristics (ROC) curves were used to establish a cut-off for absorbance that best discriminates interference.Results:The best cut-off was 0.0100 for the absorbance at the first photometric point of the complementary wavelength in the blank cuvette. Once the optimal cut-off for probable interference was selected, all samples analyzed in our laboratory that provided absorbance values above this cut-off were further investigated to try to discover paraproteins. During a period of 6 months, we detected 44 samples containing paraproteins, five of which belonged to patients with non-diagnosed monoclonal gammopathies.Conclusions:Review of the photometric reaction data permits the systematic detection of paraprotein interference on the D-Bil AU assay, even for samples for which reasonable results are obtained.
Evaluation of the Greiner Bio-One serum separator BCA Fast Clot tube
Authors: Dimeski Goce , Johnston Julie , Masci Paul P. , Zhao Kong-Nan , Brown Nigel 
Published: January 2017

AbstractBackground:Current commercial tubes have difficulties in producing “true” serum from all blood samples even within the recommended clotting times. Hence, Becton Dickinson (BD) and now Greiner have produced tubes containing thrombin as the procoagulant to reduce the clotting time and increase the possibility of producing serum from anticoagulated blood samples.Methods:The Greiner BCA Fast Clot (GBBCAFC) tube was evaluated in a hospital environment using 40 participants, (30 healthy and 10 undergoing renal dialysis) for 32 analytes against the Greiner lithium heparin tube and the BD Rapid Serum Tubes (BD RST) tube measured on Beckman DxC 800 and DxI 800 analyzers. Clotting strength was also examined using thromboelastography (TEG).Results:The analytes results showed there was a very close agreement between the BD RST tube and GBBCAFC tube in comparison with lithium heparin plasma. The result comparison data showed equivalent performance with lower levels of hemolysis. The prolonged storage study also showed very similar agreement between the BD RST and the GBBCAFC tubes. Likewise, the TEG data showed there was very little difference in clotting ability between the tubes, and neither was capable of producing true serum from blood spiked with 2 U heparin/mL of blood.Conclusions:The study showed the GBBCAFC tube with the combination of the two procoagulants blood clotting activator and thrombin produced comparable performance with the lithium heparin plasma and the BD RST serum samples.
On a class of generalized Meijer–Laplace transforms of Fox function type kernels and their extension to a class of Boehmians
Authors: Al-Omari Shrideh Khalaf Qasem 
Published: January 2017

Abstract
In this paper, we investigate a Meijer–Laplace transform enfolding Fox’s H-functions on a class of Boehmians.
The extended Meijer–Laplace transform of a Boehmian is determined and executed to preserve certain properties of the classical transform.
The inverse problem and related theorems are also discussed in some details.
Titania-Loaded Coal Char as Catalyst in Oxidation of Styrene with Aqueous Hydrogen Peroxide
Authors: Nurhadi Mukhamad , Chandren Sheela , Yuan Lai Sin , Ho Chin Siong , Indra Mahlia Teuku Meurah , Nur Hadi 
Published: January 2017

Abstract
Titania-loaded coal char catalyst was successfully prepared. The preparation steps involved pyrolysis of low rank coal at different temperatures and durations, sulfonation, impregnation of titanium(IV) isopropoxide, and then heating at 110 °C. It is found that the coal chars’ surfaces were rough after sulfonation and impregnation, while large pore volume, high surface area and carbon composition were observed at low pyrolysis temperature for short duration. These properties contributed to high selectivity towards benzaldehyde (> 90 %) at 600 °C (0.5–2 h)) in styrene oxidation using aqueous hydrogen peroxide as the oxidant.