bluuebunny/crossref_metadata_2025_split huggingface dataset:
Snake venom VEGF Vammin induces a highly efficient angiogenic response in skeletal muscle via VEGFR-2/NRP specific signaling
Authors: Toivanen Pyry I. , Nieminen Tiina , Laakkonen Johanna P. , Heikura Tommi , Kaikkonen Minna U. , Ylä-Herttuala Seppo 
Published: July 2017

AbstractVascular Endothelial Growth Factors (VEGFs) are promising molecules for the treatment of ischemic diseases by pro-angiogenic therapy. Snake venom VEGFs are a novel subgroup with unique receptor binding profiles and as such are potential new therapeutic agents. We determined the ligand-receptor interactions, gene regulation and angiogenic properties of Vipera ammodytes venom VEGF, Vammin, and compared it to the canonical angiogenic factor VEGF-A to evaluate the use of Vammin for therapeutic angiogenesis. Vammin efficiently induced VEGFR-2 mediated proliferation and expression of genes associated with proliferation, migration and angiogenesis. VEGF-A165 and especially VEGF-A109 induced less pronounced effects. Vammin regulates a number of signaling pathways by inducing the expression of NR4A family nuclear receptors and regulators of calcium signaling and MAP kinase pathways. Interestingly, MARC1, which encodes an enzyme discovered to catalyze reduction of nitrate to NO, was identified as a novel VEGFR-2 regulated gene. In rabbit skeletal muscle adenoviral delivery of Vammin induced prominent angiogenic responses. Both the vector dose and the co-receptor binding of the ligand were critical parameters controlling the type of angiogenic response from sprouting angiogenesis to vessel enlargement. Vammin induced VEGFR-2/NRP-1 mediated signaling more effectively than VEGF-A, consequently it is a promising candidate for development of pro-angiogenic therapies.
Emergence of polysaccharide membrane walls through macro-space partitioning via interfacial instability
Authors: Okeyoshi Kosuke , Okajima Maiko K. , Kaneko Tatsuo 
Published: July 2017

AbstractLiving organisms in drying environments build anisotropic structures and exhibit directionality through self-organization of biopolymers. However, the process of macro-scale assembly is still unknown. Here, we introduce a dissipative structure through a non-equilibrium process between hydration and deposition in the drying of a polysaccharide liquid crystalline solution. By controlling the geometries of the evaporation front in a limited space, multiple nuclei emerge to grow vertical membrane walls with macroscopic orientation. Notably, the membranes are formed through rational orientation of rod-like microassemblies along the dynamic three-phase contact line. Additionally, in the non-equilibrium state, a dissipative structure is ultimately immobilized as a macroscopically partitioned space by multiple vertical membranes. We foresee that such oriented membranes will be applicable to soft biomaterials with direction controllability, and the macroscopic space partitionings will aid in the understanding of the space recognition ability of natural products under drying environments.
Stability of the PHF10 subunit of PBAF signature module is regulated by phosphorylation: role of β-TrCP
Authors: Tatarskiy Victor V. , Simonov Yuriy P. , Shcherbinin Dmitrii S. , Brechalov Alexander V. , Georgieva Sofia G. , Soshnikova Nataliya V. 
Published: July 2017

AbstractThe PBAF chromatin-remodeling complexes are multi-protein machines, regulating expression of genes involved in proliferation and differentiation. PHF10 is a subunit of the PBAF essential for its association with chromatin. Mammalian PHF10 is expressed as four ubiquitous isoforms, which are alternatively incorporated in the complex and differ by their influence on transcription of target genes. PHF10 have different domain structure and two of them (PHF10-S isoforms) lack C-terminal PHD domains, which enables their phosphorylation by CK-1. Here we have found that PBAF subunits have low turnover rate, except for PHF10 which has much lower half-life, and is degraded by β-TrCP. The β-TrCP knockdown stabilizes PBAF core subunits - BRG1 and BAF155 and specific subunits - PHF10, BAF200, BAF180 and BRD7. PHF10 isoforms contain two non-canonical β-TrCP degrons and are degraded by β-TrCP in a phospho-dependent manner. But phosphorylation of PHF10-S degrons by CK-1, contrary to previously described degrons, prevents their degradation. Targeted molecular docking demonstrated that phosphorylated forms of PHF10 bind to β-TrCP with much lower affinity than non-phosphorylated ones, contrary to previously described degrons. This unorthodox mechanism proposes that phosphorylation of β-TrCP degrons by CK-1 could not only degrade a set of proteins, but also stabilize a different set of targets.
Fabricating fast triggered electro-active shape memory graphite/silver nanowires/epoxy resin composite from polymer template
Authors: Zhou Jie , Li Hua , Tian Ran , Dugnani Roberto , Lu Huiyuan , Chen Yujie , Guo Yiping , Duan Huanan , Liu Hezhou 
Published: July 2017

AbstractIn recent years shape-memory polymers have been under intense investigation due to their unique mechanical, thermal, and electrical properties that could potentially make them extremely valuable in numerous engineering applications. In this manuscript, we report a polymer-template-assisted assembly manufacturing strategy used to fabricate graphite/silver nanowires/epoxy resin (PGSE) composite. In the proposed method, the porous polymer foams work as the skeleton by forming three-dimensional graphite structure, whereas the silver nanowires act as the continuous conductive network. Preliminary testing on hybrid foams after vacuum infusion showed high electrical conductivity and excellent thermal stability. Furthermore, the composites were found to recover their original shape within 60 seconds from the application of a 0.8 V mm−1 electric field. Notably, the reported shape-memory polymer composites are manufactured with readily-available raw materials, they are fast to manufacture, and are shape-controlled.
NMR and DFT investigations of structure of colchicine in various solvents including density functional theory calculations
Authors: Pierens Gregory K. , Venkatachalam T. K. , Reutens David C. 
Published: July 2017

AbstractA detailed NMR investigation of the chemical shifts of hydrogen and carbon atoms associated with the structure of the naturally occurring alkaloid colchicine was conducted using high field NMR. Initially, the experimental chemical shifts for colchicine in chloroform and DMSO were compared to the values calculated using density functional theory (DFT). There were significant deviations observed for the chloroform solvent, but these were only slight in the DMSO solution. Dilution of the chloroform solution changed the experimental chemical shifts and improved agreement with the DFT calculations, suggesting self-aggregation at higher concentrations. A dimeric model was proposed for which agreement with the DFT calculated chemical shifts was better than for corresponding monomeric structures. Three further solvents were studied to evaluate changes in chemical shift values at different dilutions. Chloroform, benzene and water showed significant chemical shift changes implying self-aggregation, whereas DMSO and acetone did not show significant change upon dilution.
Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta
Authors: Natale Bryony V. , Schweitzer Christina , Hughes Martha , Globisch Maria A. , Kotadia Ramie , Tremblay Emilie , Vu Priscilla , Cross James C. , Natale David R. C. 
Published: July 2017

AbstractTrophoblast stem (TS) cells in the mouse derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5) to support placental development. Further development and growth is proposed to rely on layer-restricted progenitor cells. Stem cell antigen (Sca) -1 is a member of the Ly6 gene family and a known marker of stem cells in both hematopoietic and non-hematopoietic mouse tissues. Having identified that Sca-1 mRNA was highly expressed in mouse TS cells in culture, we found that it was also expressed in a subset of trophoblast within the chorion and labyrinth layer of the mouse placenta. Isolation and in vitro culture of Sca-1+ trophoblast cells from both differentiated TS cell cultures and dissected mouse placentae resulted in proliferating colonies that expressed known markers of TS cells. Furthermore, these cells could be stimulated to differentiate and expressed markers of both junctional zone and labyrinth trophoblast subtypes in a manner comparable to established mouse TS cell lines. Our results suggest that we have identified a subpopulation of TS cell-like cells that persist in the mid- to late- gestation mouse placenta as well as a cell surface protein that can be used to identify and isolate these cells.
Insulin upregulates betatrophin expression via PI3K/Akt pathway
Authors: Lu Puhan , Chen Xi , Zhang Zeqing , Zhang Jianhua , Yang Yan , Liu Zhelong , Xie Junhui , Shao Shiying , Zhou Xinrong , Hu Shuhong , He Wentao , Zhao Jiajun , Yu Xuefeng 
Published: July 2017

AbstractBetatrophin is regarded as a liver-produced hormone induced by insulin resistance (IR). However, it remains largely unknown how IR regulates betatrophin expression. To study whether IR could regulate betatrophin expression and the corresponding molecular mechanisms, betatrophin levels were examined in 6 in vitro IR models which were established using human hepatocytes L02 with different agents, including tumor necrosis factor-α, interleukin-1β, dexamethasone, palmitate, high glucose and insulin and betatrophin levels were elevated only in the insulin group. These results suggest that it is insulin, not IR that promotes betatrophin expression. In the meantime, PI3K/Akt pathway was activated by insulin and suppressed by above agents that caused IR. Insulin-upregulated betatrophin expression was suppressed by PI3K/Akt inhibitors and IR, suggesting that insulin upregulates and IR decreases betatrophin production through PI3K/Akt pathway. Consistently, the treatment of insulin in mice dose-dependently upregulated betatrophin levels, and the administration of metformin in IR mice also stimulated betatrophin production since published study showed metformin improved PI3K/Akt pathway and IR. In humans, compared with those without insulin treatment, serum betatrophin levels were increased in type 2 diabetic patients with insulin treatment. In conclusion, insulin stimulates betatrophin secretion through PI3K/Akt pathway and IR may play an opposite role.
Liquid-phase sequence capture and targeted re-sequencing revealed novel polymorphisms in tomato genes belonging to the MEP carotenoid pathway
Authors: Terracciano Irma , Cantarella Concita , Fasano Carlo , Cardi Teodoro , Mennella Giuseppe , D’Agostino Nunzio 
Published: July 2017

AbstractTomato (Solanum lycopersicum L.) plants are characterized by having a variety of fruit colours that reflect the composition and accumulation of diverse carotenoids in the berries. Carotenoids are extensively studied for their health-promoting effects and this explains the great attention these pigments received by breeders and researchers worldwide. In this work we applied Agilent’s SureSelect liquid-phase sequence capture and Illumina targeted re-sequencing of 34 tomato genes belonging to the methylerythritol phosphate (MEP) carotenoid pathway on a panel of 48 genotypes which differ for carotenoid content calculated as the sum of β-carotene, cis- and trans-lycopene. We targeted 230 kb of genomic regions including all exons and regulatory regions and observed ~40% of on-target capture. We found ample genetic variation among all the genotypes under study and generated an extensive catalog of SNPs/InDels located in both genic and regulatory regions. SNPs/InDels were also classified based on genomic location and putative biological effect. With our work we contributed to the identification of allelic variations possibly underpinning a key agronomic trait in tomato. Results from this study can be exploited for the promotion of novel studies on tomato bio-fortification as well as of breeding programs related to carotenoid accumulation in fruits.
A Fluctuation Equation of State for Prediction of High-Pressure Densities of Ionic Liquids
Authors: Chorążewski Mirosław , Postnikov Eugene B. , Jasiok Bernadeta , Nedyalkov Yuriy V. , Jacquemin Johan 
Published: July 2017

AbstractDuring this work, we demonstrate, for the first time, that the volumetric properties of pure ionic liquids could be truly predicted as a function of temperature from 219 K to 473 K and pressure up to 300 MPa. This has been achieved by using only density and isothermal compressibility data at atmospheric pressure through the Fluctuation Theory-based Tait-like Equation of State (FT-EoS). The experimental density data of 80 different ionic liquids, described in the literature by several research groups as a function of temperature and pressure, was then used to provide comparisons. Excellent predictive capability of FT-EoS was observed with an overall relative absolute average deviation close to 0.14% for the 15,298 data points examined during this work.
A global multiproxy database for temperature reconstructions of the Common Era
Authors:   PAGES2k Consortium, Emile-Geay Julien , McKay Nicholas P. , Kaufman Darrell S. , von Gunten Lucien , Wang Jianghao , Anchukaitis Kevin J. , Abram Nerilie J. , Addison Jason A. , Curran Mark A.J. , Evans Michael N. , Henley Benjamin J. , Hao Zhixin , Martrat Belen , McGregor Helen V. , Neukom Raphael , Pederson Gregory T. , Stenni Barbara , Thirumalai Kaustubh , Werner Johannes P. , Xu Chenxi , Divine Dmitry V. , Dixon Bronwyn C. , Gergis Joelle , Mundo Ignacio A. , Nakatsuka Takeshi , Phipps Steven J. , Routson Cody C. , Steig Eric J. , Tierney Jessica E. , Tyler Jonathan J. , Allen Kathryn J. , Bertler Nancy A.N. , Björklund Jesper , Chase Brian M. , Chen Min-Te , Cook Ed , de Jong Rixt , DeLong Kristine L. , Dixon Daniel A. , Ekaykin Alexey A. , Ersek Vasile , Filipsson Helena L. , Francus Pierre , Freund Mandy B. , Frezzotti Massimo , Gaire Narayan P. , Gajewski Konrad , Ge Quansheng , Goosse Hugues , Gornostaeva Anastasia , Grosjean Martin , Horiuchi Kazuho , Hormes Anne , Husum Katrine , Isaksson Elisabeth , Kandasamy Selvaraj , Kawamura Kenji , Kilbourne K. Halimeda , Koç Nalan , Leduc Guillaume , Linderholm Hans W. , Lorrey Andrew M. , Mikhalenko Vladimir , Mortyn P. Graham , Motoyama Hideaki , Moy Andrew D. , Mulvaney Robert , Munz Philipp M. , Nash David J. , Oerter Hans , Opel Thomas , Orsi Anais J. , Ovchinnikov Dmitriy V. , Porter Trevor J. , Roop Heidi A. , Saenger Casey , Sano Masaki , Sauchyn David , Saunders Krystyna M. , Seidenkrantz Marit-Solveig , Severi Mirko , Shao Xuemei , Sicre Marie-Alexandrine , Sigl Michael , Sinclair Kate , St. George Scott , St. Jacques Jeannine-Marie , Thamban Meloth , Kuwar Thapa Udya , Thomas Elizabeth R. , Turney Chris , Uemura Ryu , Viau Andre E. , Vladimirova Diana O. , Wahl Eugene R. , White James W.C. , Yu Zicheng , Zinke Jens 
Published: July 2017

AbstractReproducible climate reconstructions of the Common Era (1 CE to present) are key to placing industrial-era warming into the context of natural climatic variability. Here we present a community-sourced database of temperature-sensitive proxy records from the PAGES2k initiative. The database gathers 692 records from 648 locations, including all continental regions and major ocean basins. The records are from trees, ice, sediment, corals, speleothems, documentary evidence, and other archives. They range in length from 50 to 2000 years, with a median of 547 years, while temporal resolution ranges from biweekly to centennial. Nearly half of the proxy time series are significantly correlated with HadCRUT4.2 surface temperature over the period 1850–2014. Global temperature composites show a remarkable degree of coherence between high- and low-resolution archives, with broadly similar patterns across archive types, terrestrial versus marine locations, and screening criteria. The database is suited to investigations of global and regional temperature variability over the Common Era, and is shared in the Linked Paleo Data (LiPD) format, including serializations in Matlab, R and Python.
Non‐isolated high step‐up DC/DC converter based on a high degrees of freedom voltage gain cell
Authors: Zhu Binxin , Liu Song , Huang Yuehua , Tan Chao 
Published: July 2017

This study presents a non‐isolated high step‐up DC/DC converter based on a high degree of freedom voltage gain cell (HDF‐VGC). Both voltage stress of components and conversion ratio of the proposed converter could be adjusted by the number of the HDF‐VGC, the current stress of the semiconductor devices can also be adjusted by the input phase numbers of the converter. Moreover, the input current of each phase are automatically balanced. These characteristics make the proposed converter very suitable for high‐power and high‐voltage applications, such as offshore wind power high‐voltage DC transmission system. In this study, the topology construction of the proposed converter has been discussed. The operation principle and performance characteristics of the converter are analysed with four input phases and five HDF‐VGCs, then the converter is simulated and a 400 W experimental prototype has been built to verify the correctness of the theoretical analysis.
Wie kam die Ätiologie in die Homöopathie?
Authors: Gnaiger-Rathmanner Jutta 
Published: July 2017

ZusammenfassungDem Inhalt nach ist die Ätiologie schon im § 5 des Organon von Hahnemann begründet. Darin wird der Anfang einer Krankheit im Gegensatz zu ihrer tiefer liegenden Ursache behandelt. Der Begriff der Ätiologie selbst entwickelt sich über den Beitrag von Bönninghausens, über Hering und Kent stufenweise, bis Schmidt ihn deutlich formuliert und in seinem Alterswerk an die erste Stelle des Hierarchisierens stellt. Dorcsi formt die Ätiologie als wichtigen Baustein in seinem Lehrgebäude der Wiener Schule aus.In den neuen Repertorien finden sich ganz selbstverständlich die wertvollen Rubriken „ailments from“, „Beschwerden durch“.
Entwicklung von Maßnahmen zur Überwindung von Barrieren bezüglich einer palliativmedizinischen Versorgung von Patienten mit chronischer Herzinsuffizienz: Eine Delphi-Studie
Authors: Ziehm Jeanette , Farin Erik , Becker Gerhild , Köberich Stefan 
Published: July 2017

ZusammenfassungObwohl Patienten mit chronischer Herzinsuffizienz (CHI) unter ähnlicher Symptomlast leiden wie Patienten mit malignen Erkrankungen, werden sie weitaus seltener einer palliativmedizinischen Versorgung zugeführt. Studien begründen dies mit dem zyklischen Verlauf der Erkrankung, der den Zeitpunkt nicht eindeutig bestimmbar macht, Unkenntnis um Inhalte und Strukturen der Palliativmedizin (PM) sowie mangelnder interprofessioneller Kommunikation. Gleichwohl legen Studienergebnisse nahe, dass Patienten mit CHI von einer palliativmedizinischen Versorgung profitieren. In der vorliegenden Delphi-Befragung haben akademische Experten und klinisch erfahrene Praktiker Maßnahmen entwickelt, um zuvor identifizierte Barrieren zu überwinden und diese nach Relevanz und Umsetzbarkeit bewertet. Maßnahmen, die als relevant und umsetzbar bewertet wurden, wurden zusätzlich hinsichtlich ihrer Wichtigkeit in eine Rangfolge gebracht. Die Maßnahmen beziehen sich hauptsächlich auf bessere Fort- und Weiterbildung der Behandler sowie auf eine engere Kooperation der an der Behandlung beteiligten Akteure.
Combined epigenetic and differentiation-based treatment inhibits neuroblastoma tumor growth and links HIF2α to tumor suppression
Authors: Westerlund Isabelle , Shi Yao , Toskas Konstantinos , Fell Stuart M. , Li Shuijie , Surova Olga , Södersten Erik , Kogner Per , Nyman Ulrika , Schlisio Susanne , Holmberg Johan 
Published: July 2017

SignificanceHigh-risk neuroblastoma remains a therapeutic challenge, and adjuvant retinoic acid (RA) treatment shows poor efficacy. We demonstrate that combined treatment with 5-Aza-deoxycytidine (AZA) and RA impedes neuroblastoma growth and induces a transcriptional response characterized by high levels of the HIF2α transcription factor. This approach targets high-risk neuroblastoma that responds poorly to RA. In addition, genome-wide analysis of treated tumors and patient data links HIF2α to tumor suppression, which is supported by a HIF2α-specific small molecule inhibitor-mediated block of the tumor response to AZA+RA treatment.
Interannual cycles of Hantaan virus outbreaks at the human–animal interface in Central China are controlled by temperature and rainfall
Authors: Tian Huaiyu , Yu Pengbo , Cazelles Bernard , Xu Lei , Tan Hua , Yang Jing , Huang Shanqian , Xu Bo , Cai Jun , Ma Chaofeng , Wei Jing , Li Shen , Qu Jianhui , Laine Marko , Wang Jingjun , Tong Shilu , Stenseth Nils Chr. , Xu Bing 
Published: July 2017

Significance
Interannual cycles of many zoonotic diseases are considered to be driven by climate variability. However, the role of climate forcing in the modulation of zoonotic dynamics has been highly controversial, chiefly due to the difficulty in quantifying the links between climate forcing, animal population dynamics, and disease dynamics. Here, we address this issue by using a unique field surveillance dataset from Central China, covering one-half century. Our results shed light on the drivers behind interannual variability and the dynamic patterns of disease ecology, and the links between interannual climate variability and the human–animal interface, adding up to 3-mo lead time over outbreak warnings.
Inhibition of the integrated stress response reverses cognitive deficits after traumatic brain injury
Authors: Chou Austin , Krukowski Karen , Jopson Timothy , Zhu Ping Jun , Costa-Mattioli Mauro , Walter Peter , Rosi Susanna 
Published: July 2017

Significance
Traumatic brain injury (TBI) is a leading cause of long-term neurological disability and affects an ever-growing population. Currently, there are no effective treatments for patients suffering from chronic TBI-induced cognitive impairments. Here, we found that suppression of the integrated stress response (ISR) with a drug-like small-molecule inhibitor, ISRIB, rescued cognition in two TBI mouse models, even when administered weeks after injury. Consistent with the behavioral results, ISRIB restored long-term potentiation deficits observed in TBI mice. Our data suggest that targeting ISR activation could serve as a promising approach for the treatment of chronic cognitive dysfunction after TBI.
Influence of Temperature on the Efficacy of Homologous and Heterologous DNA Vaccines against Viral Hemorrhagic Septicemia in Pacific Herring
Authors: Hart Lucas M. , Lorenzen Niels , Einer-Jensen Katja , Purcell Maureen K. , Hershberger Paul K. 
Published: July 2017

Abstract
Homologous and heterologous (genogroup Ia) DNA vaccines against viral hemorrhagic septicemia virus (genogroup IVa) conferred partial protection in Pacific Herring Clupea pallasii. Early protection at 2 weeks postvaccination (PV) was low and occurred only at an elevated temperature (12.6°C, 189 degree days), where the relative percent survival following viral exposure was similar for the two vaccines (IVa and Ia) and higher than that of negative controls at the same temperature. Late protection at 10 weeks PV was induced by both vaccines but was higher with the homologous vaccine at both 9.0°C and 12.6°C. Virus neutralization titers were detected among 55% of all vaccinated fish at 10 weeks PV. The results suggest that the immune response profile triggered by DNA vaccination of herring was similar to that reported for Rainbow Trout Oncorhynchus mykiss by Lorenzen and LaPatra in 2005, who found interferon responses in the early days PV and the transition to adaptive response later. However, the protective effect was far less prominent in herring, possibly reflecting different physiologies or adaptations of the two fish species.
Received August 1, 2016; accepted March 10, 2017 Published online July 11, 2017