bluuebunny/crossref_metadata_2025_split huggingface dataset:
Properties of NeoMTA Plus and MTA Plus cements for endodontics
Authors: Siboni F. , Taddei P. , Prati C. , Gandolfi M. G. 
Published: April 2017

AbstractAimTo test a novel calcium silicate cement mixed with a water‐based gel (NeoMTA Plus) with regard to chemical–physical properties and apatite‐forming ability.MethodologyNeoMTA Plus (Avalon Biomed Inc. Bradenton, FL, USA; lot. 2014090301) and a commercial MTA‐based material with similar properties (MTA Plus, Prevest Denpro Limited, Jammu, India; lot. 41001) were tested for ion‐releasing ability, initial and final setting times, radiopacity, open and impervious porosity and apparent porosity, water sorption, weight loss, solubility, ability to nucleate calcium phosphates (CaP) after immersion in HBSS (Hank's Balanced Salt Solution) by ESEM‐EDX and micro‐Raman spectroscopy. The results were analysed statistically with the anova test (P  <  0.05).ResultsNeoMTA Plus had a prolonged setting time (315 min) and a satisfactory radiopacity (3.76 mm Al). Calcium and hydroxyl ion release was significantly greater and more prolonged in comparison with MTA Plus (P < 0.05). Both NeoMTA Plus and MTA Plus had high values of open porosity and solubility. ESEM‐EDX and micro‐Raman confirmed the ability to nucleate calcium phosphates on their surface after immersion in HBSS.ConclusionNeoMTA Plus is a new calcium silicate‐based cement for root filling with an adequate radiopacity and prolonged setting time. The ion release and CaP‐forming ability could increase stability of the root filling and promote endodontic and periodontal tissue regeneration, enhancing the bioactivity and biocompatibility of the material.
Wine authentication with Fourier Transform Infrared Spectroscopy: a feasibility study on variety, type of barrel wood and ageing time classification
Authors: Basalekou Marianthi , Pappas Christos , Tarantilis Petros , Kotseridis Yorgos , Kallithraka Stamatina 
Published: April 2017

AbstractWine aging in wooden barrels improves the final products’ sensory profile and increases the price. Among the different types of woods, the International Enological Codex of the International Organisation of Vine and Wine (OIV) approves only the use of Oak and Chestnut, thus producing a need for fast and cost‐effective authentication methods. In this study Fourier Transform (FT)‐mid‐infrared (IR) spectroscopy combined with chemometrics was employed to analyze and discriminate wines aged in barrels made from different type of wood species and in stainless steel tanks, over a period of 12 months. The wines used were made from four indigenous Greek grape varieties, two white and two red. A complete differentiation of the samples was achieved according to grape variety, the container type and the aging time based on two spectral regions (from 1800 to 1500 cm−1 and from 1300 to 900 cm−1) of their FT‐IR spectra.
Human adipose stromal cell therapy improves survival and reduces renal inflammation and capillary rarefaction in acute kidney injury
Authors: Collett Jason A. , Traktuev Dmitry O. , Mehrotra Purvi , Crone Allison , Merfeld‐Clauss Stephanie , March Keith L. , Basile David P. 
Published: April 2017

AbstractDamage to endothelial cells contributes to acute kidney injury (AKI) by causing impaired perfusion, while the permanent loss of the capillary network following AKI has been suggested to promote chronic kidney disease. Therefore, strategies to protect renal vasculature may impact both short‐term recovery and long‐term functional preservation post‐AKI. Human adipose stromal cells (hASCs) possess pro‐angiogenic and anti‐inflammatory properties and therefore have been tested as a therapeutic agent to treat ischaemic conditions. This study evaluated hASC potential to facilitate recovery from AKI with specific attention to capillary preservation and inflammation. Male Sprague Dawley rats were subjected to bilateral ischaemia/reperfusion and allowed to recover for either two or seven days. At the time of reperfusion, hASCs or vehicle was injected into the suprarenal abdominal aorta. hASC‐treated rats had significantly greater survival compared to vehicle‐treated rats (88.7% versus 69.3%). hASC treatment showed hastened recovery as demonstrated by lower creatinine levels at 48 hrs, while tubular damage was significantly reduced at 48 hrs. hASC treatment resulted in a significant decrease in total T cell and Th17 cell infiltration into injured kidneys at 2 days post‐AKI, but an increase in accumulation of regulatory T cells. By day 7, hASC‐treated rats showed significantly attenuated capillary rarefaction in the cortex (15% versus 5%) and outer medulla (36% versus 18%) compared to vehicle‐treated rats as well as reduced accumulation of interstitial alpha‐smooth muscle actin‐positive myofibroblasts. These results suggest for the first time that hASCs improve recovery from I/R‐induced injury by mechanisms that contribute to decrease in inflammation and preservation of peritubular capillaries.
Titanium implant functionalization with phosphate‐containing polymers may favour in vivo osseointegration
Authors: Cardoso Marcio V. , de Rycker Judith , Chaudhari Amol , Coutinho Eduardo , Yoshida Yasuhiro , Van Meerbeek Bart , Mesquita Marcelo F. , da Silva Wander J. , Yoshihara Kumiko , Vandamme Katleen , Duyck Joke 
Published: April 2017

AbstractAimOsseointegration of titanium implants is predictable, but can be improved via surface functionalization.Materials and MethodsOne hundred and twenty implants were installed in parietal bone of 12 domestic pigs and left to heal for 1 or 3 months. Five groups were defined according surface treatments: immersion in water (H2O), 10% polyphosphoric acid (PPA10), 1% phosphorylated pullulan (PPL1), 10% phosphorylated pullulan (PPL10) or 10% phosphorylated pullulan + 1 μg bone morphogenetic protein‐2 (PPL10 BMP). As primary outcome, implant osseointegration was evaluated by quantitative histology, namely peri‐implant bone formation (B/T in %) and bone‐to‐implant contact (BIC in %) for each healing period. The Wilcoxon signed‐rank test and Mann–Whitney U‐test with α = 0.05 were performed.ResultsPPL10 and PPA10 groups showed significantly higher B/T and BIC results than the control (H2O) group at 1‐month (p < .05). No significant difference was found between PPL1 and H2O or between PPL10 BMP and H2O, irrespective of healing time (1 or 3 months) or investigated parameter (B/T and BIC; p > .05). After 3 months, no experimental group showed a significant difference compared to the control group (H2O) for both investigated parameters (B/T and BIC; p > .05).ConclusionFunctionalizing titanium implants with inorganic or organic phosphate‐containing polymers at 10 wt% concentration may stimulate peri‐implant bone formation and implant osseointegration at early healing times.
Peri‐implant bone density around implants of different lengths: A 3‐year follow‐up of a randomized clinical trial
Authors: Sahrmann Philipp , Schoen Patrizia , Naenni Nadja , Jung Ronald , Attin Thomas , Schmidlin Patrick R. 
Published: April 2017

Abstract
ObjectivesShort dental implants are frequently placed, however, little is known about the effect of the loading force regarding an enhanced crown‐to‐implant ratio. The aim of this study was therefore to assess bone density changes after a 3‐year period, on radiographs acquired from a randomized, controlled two‐centre clinical study comparing implants of 6 and 10 mm of length.Materials and MethodsThree predefined areas were chosen on standardized X‐rays in order to assess grey‐scale values of the peri‐implant bone: One at the tip of the apex and two at half‐length on the mesial and distal sides of the implant. Radiographs at all follow‐up appointments had previously been calibrated using control fields in areas of constant density.ResultsAround short implants, peri‐implant bone displayed significantly higher differences in grey‐scale values (p = .031) after 3 years, indicating a higher degree of mineralization. This phenomenon was not observed around long implants.ConclusionsA higher degree of mineralization around short implants was recorded. Whether this finding goes along with hampered bone adaptability, and accordingly, higher failure rates of short implants must be studied further in long‐term clinical trials.
Repair of large saddle defects of the mandibular ridge using dual growth factor release—An experimental pilot study in minipigs
Authors: Tröltzsch Markus , Klenke Alexander , Santander Petra , Kauffmann Philipp , Tröltzsch Matthias , Rau Anna , Brockmeyer Phillip , Schliephake Henning 
Published: April 2017

AbstractAimTo test the hypothesis that the addition of small amounts of rhVEGFto rhBMP2 in a polymer carrier can accomplish equivalent repair effect as a reduced dosage of rhBMP2 compared to rhBMP2 alone.Materials and MethodsDefects were created bilaterally in the mandibles of 18 minipigs. In 12 test animals, defects were filled with 0.5 g particulatePDLLA/CaCO3 composite loaded with 400 μg rhBMP2/50 μg rhVEGF165 on one side and 800 μg rhBMP2 on the other. After 4 and 13 weeks, the animals were evaluated each for area of new bone formation (mm²) and bone density (area %).ResultsArea of newly formed bone was higher in defects with carriers loaded with 400 μg rhBMP2 50 µg VEGF165 than in defects with 800 μg rhBMP2 after 4 weeks (11.97 versus 7.97 mm²;p = 0.043) and 13 weeks (72.48 versus 62.2 mm²;p = .028). Defects filled with blank carrier exhibited less bone after 13 weeks (42.75 mm²;p = .039 and .020 respectively).ConclusionsDelivery of rhBMP2 from a polymer carrier can improve repair of large saddle defects of the mandibular ridge. Addition of small amounts of rhVEGFcan increase bone formation and at the same time reduce the dosage of rhBMP2.
Low‐Income Childless Young Adults' Marriage and Fertility Frameworks
Authors: Rackin Heather M. , Gibson‐Davis Christina M. 
Published: April 2017

AbstractThe authors investigated how low‐income young adults without children understand marriage and fertility. Data come from the Becoming Partners and Parents Study (N = 69), a qualitative study of African American adults aged 18 to 22 in a mid‐size southern city. This is the first study to analyze young, low‐income, childless, and unmarried Black respondents' frameworks (i.e., internal understandings of the world) of marriage and fertility. In contrast to research conducted on parents, this research on childless adults indicated a narrative in which there were close connections between marriage and fertility and an economic bar adhered to both marriage and childbearing. Respondents also believed that childbearing was meaningful and provided purpose, but that it was morally questionable if the parent was not financially stable. The results suggest that prior findings related to meanings of family formation and childbearing for low‐income parents may not extend to those without children.
Outcome of three screening questions for temporomandibular disorders (3Q/TMD) on clinical decision‐making
Authors: Lövgren A. , Marklund S. , Visscher C. M. , Lobbezoo F. , Häggman‐Henrikson B. , Wänman A. 
Published: April 2017

SummaryPatients with temporomandibular disorders (TMD) seem to go undetected and not adequately managed within dentistry. To identify these patients, three screening questions (3Q/TMD) have been introduced within dentistry in parts of Sweden. It is not known whether 3Q/TMD affects the clinical decision‐making for these patients. The aim of this study was to evaluate the outcome of 3Q/TMD on the clinical decision‐making and to analyse whether gender, age and the fee system the individual was assigned to were related to prescribed TMD treatment. This cohort study was carried out within the Public Dental Health service in Västerbotten, Sweden. As part of the routine dental check‐up, a health declaration including 3Q/TMD was completed. The study population was randomly selected based on their 3Q/TMD answers. In total, 300 individuals with an affirmative answer to any of the 3Q/TMD, and 500 individuals with all negative answers were selected. The 3Q/TMD includes questions on weekly jaw‐face‐temple pain (Q1), pain on function (Q2) and catching/locking of the jaw (Q3). The 3Q/TMD was analysed in relation to prescribed treatment assessed from dental records. There was significantly more treatment performed or recommended for 3Q‐positives (21·5%), compared to 3Q‐negatives (2·2%) (P < 0·001). The odds ratio for TMD‐related treatment for 3Q‐positives versus 3Q‐negatives was 12·1 (95% CI: 6·3–23·4). Although affirmative answers to the 3Q/TMD was related to TMD treatment, the majority of individuals with a screen positive still did not, according to dental records, receive assessment or treatment. Further studies are needed to better understand the clinical decision‐making process for patients with TMD.
Host range, mating type and population structure of Magnaporthe sp. of a single barley field in São Paulo state, Brazil
Authors: Urashima Alfredo Seiiti , Alves Augusto Freitas , Silva Flávio Nunes , Oliveira Danielle , Gazaffi Rodrigo 
Published: April 2017

AbstractBrazil is blast disease hot spot because severe epidemics have occurred among wheat, triticale, rye, barley and oat crops. Although the first outbreak of barley blast appeared in 1998, little information is available. Therefore, this study aimed to examine host range, mating type composition and population structure of Magnaporthe sp. from a single barley field in São Paulo, Brazil. To examine pathogenicity, 25 Magnaporthe isolates were inoculated on five, three, two and two cultivars of barley, wheat, oat and rice, respectively, and one cultivar each of rye, corn, sorghum, triticale and certain weeds (Cenchrus echinatus, Setaria geniculata, Brachiaria plantaginea and Eleusine indica). Mating type distribution of 33 isolates was investigated by molecular tools. The genotypic divergence of 41 barley and five wheat isolates was investigated by 15 random amplified polymorphic DNA primers and unweighted pair group method with arithmetic mean. The host range of the barley blast pathogen included wheat, oat, rye and triticale but not rice and weeds. Sexual reproduction appeared to not be involved in the high genotypic diversity because only a single isolate, MAT1‐2, was identified. The majority of barley isolates clustered together with wheat blast, except for four, suggesting a different origin.
A comparison of UVb compact lamps in enabling cutaneous vitamin D synthesis in growing bearded dragons
Authors: Diehl J. J. E. , Baines F. M. , Heijboer A. C. , van Leeuwen J. P. , Kik M. , Hendriks W. H. , Oonincx D. G. A. B. 
Published: April 2017

SummaryThe effect of exposure to different UVb compact lamps on the vitamin D status of growing bearded dragons (Pogona vitticeps) was studied. Forty‐two newly hatched bearded dragons (<24 h old) were allocated to six treatment groups (n = 7 per group). Five groups were exposed to different UVb compact lamps for two hours per day, with a control group not exposed to UVb radiation. At 120 days of age, blood samples were obtained and concentrations of 25(OH)D3, Ca, P and uric acid were determined. In addition, plasma 25(OH)D3 concentration was determined in free‐living adult bearded dragons to provide a reference level. Only one treatment resulted in elevated levels of 25(OH)D3 compared to the control group (41.0 ± 12.85 vs. 2.0 ± 0.0 nmol/L). All UVb‐exposed groups had low 25(OH)D3 plasma levels compared to earlier studies on captive bearded dragons as well as in comparison with the free‐living adult bearded dragons (409 ± 56 nmol/L). Spectral analysis indicated that all treatment lamps emitted UVb wavelengths effective for some cutaneous vitamin D synthesis. None of these lamps, under this regime, appeared to have provided a sufficient UVb dose to enable synthesis of plasma 25(OH)D3 levels similar to those of free‐living bearded dragons in their native habitat.
Pharmacokinetics of firocoxib after intravenous administration of multiple consecutive doses in neonatal foals
Authors: Wilson K. E. , Davis J. L. , Crisman M. V. , Kvaternick V. , Zarabadipour C. , Cheramie H. , Hodgson D. R. 
Published: April 2017

The purpose of this study was to determine the pharmacokinetic profile of intravenous firocoxib in neonatal foals. Six healthy foals were administered 0.09 mg/kg firocoxib intravenously once a day for 7 days. Blood was collected for plasma firocoxib analysis using high‐performance liquid chromatography with fluorescence detection at times 0 (day 1 of study only) and 0.08, 0.25, 1, 2, 4, 6, 8, 16 and 24 hr on dose numbers 1, 5 and 7. Blood was also collected immediately prior to doses 3, 4, 5 and 7. Final samples were collected at 36, 48, 72 and 96 hr following the final dose. Noncompartmental analysis using the trapezoidal method with linear interpolation revealed a moderate half‐life (15.9 ± 9.1 hr) with a large volume of distribution at steady state (1.79 ± 0.57 L/kg) and a clearance (96.0 ± 59.2 ml h−1 kg−1) that was more rapid than that observed in adult horses.
Stomatal function, density and pattern, and CO2 assimilation in Arabidopsis thaliana tmm1 and sdd1‐1 mutants
Authors: Vráblová M. , Vrábl D. , Hronková M. , Kubásek J. , Šantrůček J. 
Published: April 2017

Abstract

Stomata modulate the exchange of water and CO2 between plant and atmosphere. Although stomatal density is known to affect CO2 diffusion into the leaf and thus photosynthetic rate, the effect of stomatal density and patterning on CO2 assimilation is not fully understood.

We used wild types Col‐0 and C24 and stomatal mutants sdd1‐1 and tmm1 of Arabidopsis thaliana, differing in stomatal density and pattern, to study the effects of these variations on both stomatal and mesophyll conductance and CO2 assimilation rate. Anatomical parameters of stomata, leaf temperature and carbon isotope discrimination were also assessed.

Our results indicate that increased stomatal density enhanced stomatal conductance in sdd1‐1 plants, with no effect on photosynthesis, due to both unchanged photosynthetic capacity and decreased mesophyll conductance. Clustering (abnormal patterning formed by clusters of two or more stomata) and a highly unequal distribution of stomata between the adaxial and abaxial leaf sides in tmm1 mutants also had no effect on photosynthesis.

Except at very high stomatal densities, stomatal conductance and water loss were proportional to stomatal density. Stomatal formation in clusters reduced stomatal dynamics and their operational range as well as the efficiency of CO2 transport.
The influence of delipidation on triglyceride and LIPIN1 of porcine embryos derived from parthenogenetic activation
Authors: Zeng Y , Wang C , Niu Y , Chi D , Xu M , Si L , Qu X , Li J 
Published: April 2017

ContentsProteins in the LIPIN family play key roles in lipid synthesis mainly on triacylglycerol (TAG) biosynthesis, and they also act as transcriptional coactivators to regulate the expression of genes involved in lipid metabolism with other nuclear factors. Hence, this study was designed to investigate LIPIN1 in pig oocytes and embryos by the delipidation. After delipidation, the content of lipids (LDs) and TAG in MII oocyte was significantly reduced; however, a similar increasing tendency of TAG was shown during embryos development. Subsequently, the expression of genes related to TAG biosynthesis including GPAT1, AGPAT1, AGPAT2, LIPIN1, DGAT and the nuclear factors interacted with LIPIN1 including PPARα and PPARγ was investigated. It is obvious that DGAT and GPAT1, and LIPIN1 increased significantly after delipidation at 1‐cell and 4‐cell stage, and the expression of PPARα and PPARγ also increased at 4‐cell stage. By immunofluorescence staining and Western blots, LIPIN1 was found to exhibit a dynamic localization pattern and gradually increase with the development of delipated embryo. In the early developmental stages (1‐, 2‐ and 4‐cell stages), it was distributed over the cortical layer. But at the blastocyst stage, a homogeneous distribution of LIPIN1 was observed in cytoplasm. At 2‐cell stage, the expression of PPARα decreased when LIPIN1 was interfered by small interfering RNA, but PPARγ has no significant difference. Therefore, in this study, we find after delipidation, the content of TAG and LIPIN1 will gradually increase during embryo development and nuclear factor PPARα and PPARγ can also be affected by delipidation. The interaction of LIPIN1 and PPARα exists in porcine embryo.
Coccidioidomycosis among persons undergoing lung transplantation in the coccidioidal endemic region
Authors: Chaudhary Sachin , Meinke Laura , Ateeli Huthayfa , Knox Kenneth S. , Raz Yuval , Ampel Neil M. 
Published: April 2017

AbstractBackgroundCoccidioidomycosis, an endemic fungal infection, is more likely to be symptomatic and severe among those receiving allogeneic transplants. While several case series have been published for various transplanted organs, none has described the incidence and outcomes in those receiving lung transplants within the coccidioidal endemic region.MethodsPatients receiving a heart‐lung, single‐lung, or bilateral‐lung transplantation at the University of Arizona between 1985 and 2009 were retrospectively reviewed.ResultsCoccidioidomycosis occurred post transplantation in 11 (5.8%) of 189 patients. All but one patient was diagnosed with pulmonary coccidioidomycosis and only one had a history of prior coccidioidomycosis. Two patients received transplants from donors found to have coccidioidomycosis at the time of transplantation and one death was directly attributed to coccidioidomycosis. The risk of developing active coccidioidomycosis was significantly higher if the patient did not receive some type of antifungal therapy post transplantation (P<.001).ConclusionWithin the coccidioidal endemic region, post‐transplantation coccidioidomycosis was a definable risk among lung transplant recipients. Use of antifungals appeared to reduce this incidence of disease. Almost all cases resulted in pulmonary disease, suggesting that the lung is the primary site of infection.