bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of firocoxib on cyclooxygenase 2, microsomal prostaglandin E2 synthase 1, and cytosolic phospholipase A2 gene expression in equine mononuclear cells
Authors: Barton Michelle H. , Darden Joshua E. , Clifton Sarah , Vandenplas Michel 
Published: November 2015

Abstract
OBJECTIVE To validate primer sets for use in reverse transcription quantitative PCR assays to measure gene expression of cytosolic phospholipase A2 (cPLA2) and microsomal prostaglandin E2 synthase 1 (mPGES1) in equine mononuclear cells and determine the effects of firocoxib, a selective cyclooxygenase 2 (COX-2) inhibitor, on COX-2, cPLA2, and mPGES1 gene expression following incubation of mononuclear cells with lipopolysaccharide (LPS).
ANIMALS 8 healthy adult horses.
PROCEDURES Peripheral blood mononuclear cells were isolated by density gradient centrifugation and incubated at 37°C with medium alone, firocoxib (100 ng/mL), LPS (1 ng/mL or 1 μg/mL), or combinations of firocoxib and both LPS concentrations. After 4 hours, supernatants were collected and tested for prostaglandin E2 (PGE2) concentration with an enzyme inhibition assay, and gene expression in cell lysates was measured with PCR assays.
RESULTS Primer pairs for cPLA2 and mPGES1 yielded single products on dissociation curve analyses, with mean assay efficiencies of 102% and 100%, respectively. Incubation with firocoxib and LPS significantly decreased PGE2 supernatant concentrations and significantly reduced COX-2 and mPGES1 gene expression, compared with values following incubation with LPS alone.
CONCLUSIONS AND CLINICAL RELEVANCE Primer sets for mPGES1 and cPLA2 gene expression in equine mononuclear cells were successfully validated. Firocoxib significantly decreased LPS-induced COX-2 and mPGES1 expression, suggesting that it may be useful in the control of diseases in which expression of these genes is upregulated.
Two New Species of the Genus Calozodion Gardiner (Crustacea: Tanaidacea: Apseudomorpha) from the Adriatic Sea and the Indian Ocean, and the Reclassification of C. Dollfusi Guţu, 1989 in the Genus Julmarichardia Guţu/ Două specii noi din Genul Calozodion Gardiner (Crustacea: Tanaidacea: Apseudomorpha) din Marea Adriatică şi Oceanul Indian şi reclasificarea speciei C. Dollfusi Guţu, 1989 în Genul Julmarichardia Guţu
Authors: Guţu Modest 
Published: November 2015

RezumatSunt descrise două specii noi pentru ştiinţă, Calozodion bogoescui şi C. tanzaniense, provenind din apele sud-estice italiene ale Mării Adriatice şi, respectiv, cele tanzaniene ale Oceanului Indian. Prin descrierea speciei C. bogoescui n. sp. este menţionată pentru prima dată prezenţa genului Calozodion Gardiner, 1973 în bazinul mediteranean. Principalele trăsături morfologice caracteristice speciei mediteraneene constau în numărul mic al articulelor endopodului uropodal (numai şapte, în comparaţie cu 13-16, cât au celelalte specii ale genului) şi lungimea mică a merusului chelipedului (comparativ cu cea a carpului). Specia C. tanzaniense n. sp. se deosebeşte de celelalte cunoscute prin următoarea combinaţie de trăsături morfologice: (1) antenula cu trei apofize spiniforme pe marginea externă a primului articul peduncular, (2) antena formată din nouă aticule, (3) bazisul pereopodului II cu sete penate lungi numai pe marginea dorsală, (4) bazisul pereopodelor III şi IV cu câte o apofiză spiniformă pe marginea dorsală şi (5) merusul pereopodului VII cu cinci sete penate lungi pe marginea dorsală.Totodată specia C. dollfusi Guţu, 1989 este transferată în alt gen, devenind Julmarichardia dollfusi (Guţu, 1989), comb. nov., iar în cazul masculilor speciei C. dominiki Bochert, 2012 sunt comentate unele particularităţi morfologice neîntâlnite la alte specii ale genului. În partea finală a lucrării este prezentată cheia de identificare a celor 11 specii ale genului Calozodion.
An Investigation of Ant-Nest Corrosion caused by the Leakage of a Copper Tube – Finned Heat Exchanger of an Air Conditioning Unit
Authors: Gaedicke R. , Stein S. 
Published: November 2015

Abstract
Leakages often occur in the copper tube – finned heat exchangers of cooling and air conditioning units, the dimensions of which are on the microscopic scale thus making the location and cause very difficult to determine. In the case presented here the leakages found were located using a Helium leak detector. After removing the fins of the heat exchanger, because the points of failure were not visible to the naked eye, a dye penetrant test was carried out on the affected area (Penetrations test or PT), which led to the discovery of three very small indications on one section of the tube. Subsequent microscopic laboratory examination revealed the cause to be microscopically small holes in the wall of the tube caused by corrosion through from the internal surface of the coolant side of the tube. The corrosion mechanism was identified as Formicary Corrosion otherwise referred to as Ant-Nest Corrosion. For this type of corrosion to occur, which according to the specialist literature is often found in Cooling or Air Conditioning units, an aqueous electrolyte or electrolytic film containing Carbonic acid and Oxygen is required which can lead to damage occurring within weeks to months. In the case described in this article, the unintentional ingress of an aqueous oxygenated acidic solution of Glycol into the heat exchanger was most probably responsible for the damage.
Intermetallic Titanium Aluminides as Innovative High Temperature Lightweight Structural Materials – How Materialographic Methods Have Contributed to Their Development
Authors: Clemens H. , Mayer S. 
Published: November 2015

Abstract
The present article is considered the continuation of the two review articles “Intermetallische γ-Titanaluminid-Basislegierungen aus metallographischer Sicht” (“Intermetallic γ titanium aluminide based alloys from a metallographic point of view”) by H. Clemens and F. Jeglitsch in Pract. Metallogr. 37 (2000) 194 – 217 and “Intermetallische γ-Titanaluminid-Basislegierungen aus metallographischer Sicht – eine Fortsetzung” (“Intermetallic γ titanium aluminide based alloy from a metallographic point of view – a continuation”) by H. Clemens and S. Mayer in Pract. Metallogr. 48 (2011) 64 – 100. With reference to a γ TiAl based alloy specifically developed for aircraft engines, the so-called TNM alloy, this third and concluding part describes how materialographic methods, in combination with a targeted use of complementary investigation methods, have contributed to their development, manufacture, and processing, and, eventually, to their industrial launch. In this context, the alloy development strategy is discussed and representative microstructures and nanostructures are shown and described after different processing and heat treatment processes. Selected case studies from materialographic examinations are presented and interpreted using, for each and every of these aspects, approaches from the fields of physical metallurgy und metal physics.
The added value of SPECT/spiral CT in patients with equivocal bony metastasis from hepatocellular carcinoma
Authors: Shi H. , Li B. , Cai L. , Gu Y. , Xiu Y. , Zhang Y. 
Published: November 2015

SummaryThe aim of this study was to evaluate the incremental value provided by single-photon emission computed tomography (SPECT)/spiral computed tomography (CT) over SPECT alone for the evaluation of equivocal bone lesions in patients with hepatocellular carcinoma (HCC). Patients, methods: This was a retrospective study of 65 patients (61 men and 4 women; mean age, 53.1 ± 10.1 years; age range, 31–78 years) with HCC confirmed by pathology who underwent planar whole body scintigraphy and SPECT/CT. Each lesion was scored visually using a 5-point scale (0, negative; 1, benign; 2, likely benign; 3, likely bone metastasis; 4, bone metastasis). Lesions in which diagnostic confidence was scored as 3 or 4 were categorized into metastases.The final diagnosis of each lesion was based on pathological confirmation or follow-up by whole body scintigraphy and SPECT/spiral CT conducted two or more times. The diagnostic accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of SPECT alone and SPECT/CT were computed. Results: The diagnostic accuracy, sensitivity, specificity, PPV, and NPV of SPECT alone were 78.4, 70.9%, 94.9%, 96.8% and 59.7%, respectively. Moreover, for agreement of SPECT alone and the final results, the kappa value was 0.567 (p < 0.001), which was considered to be moderate agreement. The diagnostic accuracy, sensitivity, specificity, PPV, and NPV of SPECT/CT were 99.2%, 100%, 97.4%, 98.9% and 100 %, respectively. Furthermore, for agreement of SPECT/CT and the final results, the kappa value was 0.981 (p < 0.001), which was considered to be nearly perfect agreement. Conclusions: SPECT/spiral CT is more accurate and valuable than SPECT alone in the differential diagnosis of equivocal bone lesions in patients with HCC.
Maternal Vitamin D Deficiency and Fetal Growth
Authors: Ioannou Christos 
Published: November 2015

ABSTRACT
There is increasing incidence of vitamin D deficiency in pregnancy in developed countries. Dark skinned women who have migrated and live in higher latitudes are at greatest risk. Vitamin D supplementation is now recommended in several countries, yet its impact on fetal growth remains unclear. Observational studies suggest a possible correlation between maternal serum vitaminD and birth weight. However, differences in birth weight can be confounded by soft-tissue growth and placental function. The effect on vitamin D on bone mineral indices using dual energy X-ray absorptiometry is difficult to ascertain in the neonatal period and therefore remains unclear. Prenatal ultrasound is a safe and practical modality for assessing skeletal growth, yet very few studies have investigated fetal growth in the context of vitamin D status: one study has demonstrated no correlation with femur length, whereas two studies have shown positive correlations with femur length and femur volume respectively. The effect of vitamin D supplementation on birth weight has been investigated in seven interventional studies. However, there is considerable methodological heterogeneity and high risk of bias among some of them. Meta-analysis of well conducted randomized controlled trials (RCTs) has not demonstrated a significant effect on birth weight. The effect of vitamin D supplementation on ultrasound markers of fetal growth has not been reported to date. In summary, there is weak evidence that maternal vitamin D status may have a positive association with measures of fetal skeletal growth. However, if such an association exists, it is not clear whether it is causal or spurious. Randomized controlled trials of vitamin D supplementation are needed, where fetal ultrasound and neonatal bone mineral indices will be reported as primary outcome measures.

How to cite this article
Ioannou C. Maternal Vitamin D Deficiency and Fetal Growth. Donald School J Ultrasound Obstet Gynecol 2015;9(3):223-229.
Associations between the Grade of Placental Maturity at Third Trimester Ultrasound and Maternofetal Outcomes at the Maternity of the Yaoundé Central Hospital: A Prospective Cohort Study
Authors: Fouedjio Jeanne Hortence , Ymele Fouelifack Florent , Tsuala Fouogue Jovanny , Tetka Tetka Thierry 
Published: November 2015

ABSTRACT

Background
Placental maturity assessed by ultrasound has been classified into 3 grades by Grannum. Grade 3 before the 36th week of pregnancy is associated with adverse maternofetal morbidity. We sought to determine the prevalence of grade 3 placenta between the 34th and the 36th week of pregnancy and to assess its impact on maternofetal outcomes in Cameroon.

Materials and methods
This prospective cohort study was conducted from January 2012 to July 2013 in the maternity unit of the Yaoundé Central Hospital.

Results
One hundred and two women with singleton pregnancies between the 34th and 36th weeks were included. Grade 3 placenta accounted for 5.9% of our sample. Grade 3 was significantly associated with the following: anterior location of the placenta (RR = 5.36; 95% CI: 1.36–9.62; p = 0.03), pre-eclampsia (RR = 12; 95% CI: 3.52–20.8; p = 0,003), meconium-stained amniotic fluid (RR = 4.32; 95% CI: 1.65–11.51; p = 0.04) and intrauterine growth restriction (RR = 2.43; 95% CI: 1.33–15.6; p = 0.007).

Conclusion
Grade 3 placental maturity before the 36th week of pregnancy is significantly associated with some maternal and fetal morbidities. Therefore, it warrants close monitoring to detect and avoid complications.

How to cite this article
Hortence FJ, Florent FY, Fouogue JT, Thierry TT. Associations between the Grade of Placental Maturity at Third Trimester Ultrasound and Maternofetal outcomes at the Maternity of the Yaoundé Central Hospital: A Prospective Cohort Study. Donald School J Ultrasound Obstet Gynecol 2015;9(3):230-233.
Evaluation of Parameters that Influence Morbidity in Peripartum Hysterectomy
Authors: Antsaklis Panos , Daskalakis George , Pergialiotis Vasilios , Rodolakis Alexandros , Vlachos George , Loutradis Dimitrios , Papantoniou Nikolaos 
Published: November 2015

ABSTRACT

Objective
To evaluate which factors affect the intraoperative and postoperative morbidity in cases of peripartum hysterectomy.

Study design
A retrospective study of all cases of peripartum hysterectomy performed during a 5-year period (January 2008–June 2013) in a tertiary maternity hospital.

Results
A total of 22,437 deliveries were reviewed and 63 cases of peripartum hysterectomy (2.8/1000) were identified. The indications for peripartum hysterectomy included: uterine atony (10 cases—15.9%), placenta accreta (21 cases—33.3%), placenta previa (30 cases—47.6%) and cervical pregnancy (2 cases—3.2%). Significantly higher rates of perioperative blood transfusion were noted in the emergency cases group, compared to the elective hysterectomies. Hypogastric artery ligation did not have any significant impact on the outcome. Preoperative bilateral ureteral catheterization was associated with lower need for blood transfusion (p < 0.001), and with less complications, although this was not statistically significant.

Conclusion
Maternal morbidity is significantly higher in emergency cases of peripartum hysterectomies compared to expected-planned cases.

How to cite this article
Daskalakis G, Antsaklis P, PergialiotisV, Rodolakis A, Vlachos G, Loutradis D, Papantoniou N. Evaluation of Parameters that Influence Morbidity Peripartum Hysterectomy. Donald School J Ultrasound Obstet Gynecol 2015;9(3):234-238.
Invasive Prenatal Diagnosis: Amniocentesis
Authors: Antsaklis Panos , Antsaklis Aris , Theodora Mariana 
Published: November 2015

ABSTRACT
In this article, we will review the history and the evolution of the technique of amniocentesis and the indications of the most common invasive diagnostic and therapeutic procedure. Moreover, the most common complications of amniocentesis will be presented. Finally, we will try to establish if the use of concurrent ultrasound had any effect on the prevalence on these complications.

How to cite this article
Theodora M, Antsaklis P, Antsaklis A. Invasive Prenatal Diagnosis: Amniocentesis. Donald School J Ultrasound Obstet Gynecol 2015;9(3):307-313.
Bioimpedance Assessment of Oral Squamous Cell Carcinoma with Clinicopathological Correlation
Authors: Kulkarni Meena , Karmarkar Swarada 
Published: November 2015

ABSTRACT

Aim
Molecular alterations at membrane, cytosol and nuclear level in cancer cells/tissue show variations in bioimpedance measure. In the present study, bioimpedance assessment and comparison was investigated between oral squamous cell carcinoma (OSCC) and normal tissue. Study further involves clinicopathological correlation of bioimpedance values in OSCC.

Materials and methods
The present study is comprised of 50 OSCC cases and 50 healthy control subjects. Four electrical properties of OSCC were measured: Impedance (Z); Phase angle (è); Real part of impedance (R); and Imaginary part of impedance (X) at six frequencies: 20 Hz; 50 kHz; 1.3 MHz; 2.5 MHz; 3.7 MHz; and 5 MHz with the amplitude of the applied voltage limited to 200 mV.

Results
The bioimpedance of OSCC as well as control group decreased as the measurement frequency increased from 20 Hz to 5 MHz. The bioimpedance of OSCC was generally smaller than that of control group. The mean bioimpedance of OSCC was found to be 4493 ± 216.9 Ω and 370.0 ± 26.45 Ω and that of control group was 15490 ± 287.2 Ω and 817.1 ± 7.227 Ω at frequencies of 20 Hz and 50 MHz respectively which is statistically significant (p < 0.0001). The values of phase angle, real and imaginary part of impedance of OSCC group were found to be significantly larger than that of control group (p < 0.0001) at 20 Hz and 50 MHz frequency. Impedance values of OSCC were seen to decrease from stages I to IV. Statistically significant differences in values of impedance were observed between stage I (4881 ± 262.5 Ω) and IV (4500 ± 181.6 Ω) (p = 0.0060) and also between stage I (4881 ± 262.5 Ω) and III (4376 ± 121.3 Ω) at frequency of 20 Hz (p-value 0.0005). Statistically significant differences in values of impedance were also observed between well differentiated (4557 ± 260.8) and poorly differentiated OSCC (4347 ± 76.12) (p = 0.0004) but only at 20 Hz frequency.

Conclusion
Bioimpedance at a particular frequency showed significant alteration in OSCC tissue as compared to control. Hence, it can be potentially promising detection technique for OSCC.

Clinical significance
It is a low-cost real time method, which requires little training, and hence can be easily used in primary care centers or in developing countries where multiple challenges limit national screening programs.

How to cite this article
Sarode GS, Sarode SC, Kulkarni M, Karmarkar S, Patil S, Augustine D. Bioimpedance Assessment of Oral Squamous Cell Carcinoma with Clinicopathological Correlation. J Contemp Dent Pract 2015;16(9):715-722.
Sealing Ability of Four Calcium Containing Cements used for Repairing Furcal Perforations in Primary Molars: An in vitro study
Authors: El-Khodary Heba M , Farsi Deema J , Farsi Najat M , Zidan Ahmed Z 
Published: November 2015

ABSTRACT

Aim
The aim of this study was to compare the sealing ability of mineral trioxide aggregate (MTA), Portland cement (PC), BiodentineTM and Tech biosealer in repairing furcal perforations in primary molars using the fluid-filtration technique.

Materials and methods
Fifty freshly extracted maxillary second primary molars were sectioned horizontally at the furcation region to create dentin disks of 1.5 mm (+ 0.1 mm) thickness. Five disks were not perforated and served as negative controls. In the remaining 45 disks, furcation perforations were prepared. Five disks did not receive furcation repair and served as positive controls. The remaining 40 disks were then randomly divided into four equal groups (10 disks in each group). Perforations were repaired with: MTA, PC, BiodentineTM or Tech Biosealer. The sealing ability of the tested materials was evaluated by measuring microleakage for each disk after four different storage periods: 24-hour, 1-month, 6-month and 1-year storage using fluid-filtration. Comparisons between the four materials and the four time periods were done using the two-way analysis of variance and the Scheffe multiple comparisons test.

Results
There was no significant difference between the mean microleakage values obtained in the four tested materials after 24 hours, 1, 6 month and 1 year. However, microleakage values for each individual material were significantly higher at 24 hours than at the other time intervals.

Conclusion
Mineral trioxide aggregate, PC, BiodentineTM and Tech biosealer showed similar capabilities in sealing the furcal perforations of the primary molars, where the sealing ability improved over time for each individual material.

How to cite this article
El-Khodary HM, Farsi DJ, Farsi NM, Zidan AZ. Sealing Ability of Four Calcium Containing Cements used for Repairing Furcal Perforations in Primary Molars: An in vitro study. J Contemp Dent Pract 2015;16(9):733-739.
Orthodontic Extrusion as Treatment Option for Crown- Root Fracture: Literature Review with Systematic Criteria
Authors: Okamoto Roberta , de Faria Lorraine Perciliano , de Almeida Melyna Marques , Amaral Marina Fuzette , Mendonça Marcos Rogério 
Published: November 2015

ABSTRACTObjectiveTo review the literature searching for a consensus for the choice of orthodontic extrusion as treatment for crownroot fracture.Materials and methodsAn electronic search was performed in the databases PubMed, Cochrane Central Register of Controlled Trials and Scopus and a manual search of the Journal Dental Traumatology.ResultsForty articles were found in PubMed and 38 in Scopus and after removal of duplicate sample 51 contained articles. Of these, 48 were excluded for not having orthodontic treatment, no follow-up or follow-up less than 6 months, or not report the presence of crown-root fracture. In manual search in Dental Traumatology 20 articles were found, but none of them met the prerequisites established. So, three articles formed the basis of the study.ConclusionThe choice of how to treat orthodontic extrusion of crown-root fracture was effective and stable, without root and periodontal changes. Factors, such as root formation and presence of pulp vitality were decisive for determining the stages of treatment, however, there is no consensus based on scientific evidence about these protocols.How to cite this articlede Faria LP, de Almeida MM, Amaral MF, Pellizzer EP, Okamoto R, Mendonça MR. Orthodontic Extrusion as Treatment Option for Crown-Root Fracture: Literature Review with Systematic Criteria. J Contemp Dent Pract 2015;16(9):758-762.
Quality of Life related to Eruption Hematoma in a Twenty Months Old Infant
Authors: Maia Lucianne Cople , Marques Andréa Laudares , de Alencar Nashalie Andrade , Antonio Andréa Gonçalves 
Published: November 2015

ABSTRACT

Aim
This paper describes a case of an eruption hematoma in a 20-month-old boy and the impact of this hematoma on the quality of life (QoL) related to oral health of this infant and his family.

Background
Eruption hematoma is a soft benign cyst that contains blood and overlie a tooth that are about to erupt. Oral health conditions can affect the QoL and bring psychological impacts.

Case report
The proposed treatment was based on oral hygiene instruction, normal diet and massage on the lesion area. A weekly follow-up visits up to the spontaneous regression at the 6 weeks of the lesion was conducted. The impact on QoL was assessed though the Brazilian version of the early childhood Oral Health Impact Scale (B-ECOHIS) questionnaire before and after 2 months of the hematoma regression.

Conclusion
The presence of eruption hematoma impacted, physically and emotionally, the QoL related to oral health of the child and his family, and this impact decreased when the eruption hematoma disappeared.

Clinical significance
It is important the knowledge of the dentists about eruption cyst/hematoma to make the correct decisions to improve the QoL of their patients and families.

How to cite this article
Marques AL, de Alencar NA, Maia LC, Antonio AG. Quality of Life related to Eruption Hematoma in a Twenty Months Old Infant. J Contemp Dent Pract 2015;16(9): 763-767.