bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crema dental a base de las propiedades medicinales y terapéuticas del aceite esencial del geranio (Pelargonium x bortorum)
Authors: Laime Soto Susan 
Published: January 2018

Dada la importancia del geranio (Pelargonim x hortorum) en nuestra región, por ser una planta que crece abundantemente todo el año y su cultivo no necesita mayores cuidados, y presentar propiedades terapéuticas y farmacológicas excepcionales, se realizó su estudio fitoquímico para comprobar y determinar sus principios activos, los mismos que tienen efectos terapéuticos sobre la salud.El estudio comprende la purificación de los metabolitos secundarios de la planta, dentro de ellos los aceites esenciales, a través de marchas fitoquímicas para evidenciar y detectar los mismos mediante pruebas fisicoquímicas y farmacológicas. El aceite esencial del geranio, una vez aislado y purificado, se usó en la preparación de una crema dental, extractos y colutorios, los cuales tendrían la propiedad de evitar las caries dental y las enfermedadesperiodontales, sobre todo en las personas de menores recursos, por ser un producto muy económico.Se realizaron bioensayos para determinar la actividad de sus principios activos. También se realizaron análisis microbiológicos para evidenciar el efecto antibacteriano del geraniol (aceite esencial del geranio).Se comprobó que el extracto oleoso tiene un poder de inhibición considerable sobre el crecimiento bacteriano, especialmente sobre los gérmenes que causan la placa bacteriana y la caries dental. En el experimento realizado de las diez placas en estudio, nueve inhibieron por completo el crecimiento bacteriano en las zonas tratadas, y solo una no lo hizo.El control de calidad analítico se realizó partiendo de la muestra, y comprendió todas las etapas de los análisis fisicoquímicos y bacteriológicos.Palabras clave: metabolitos, aceites esenciales, geraniol.DOI: http://dx.doi.org/10.21503/CienciayDesarrollo.2008.v9.09
Edelfosine: An Antitumor Drug Prototype
Authors: Teixeira Sarah F. , Rodrigues Cecilia P. , Costa Cícero J.S. , Pettinati Thais N. , de Azevedo Ricardo A. , Mambelli Lisley I. , Jorge Salomão D. , Ramos Rodrigo N. , Ferro Emer S. , Barbuto José A.M. , Ferreira Adilson K. 
Published: January 2018

Background:
Lung cancer is the most prevalent cancer and a high fatality disease. Despite of all
available therapeutic approaches, drug resistance of chemotherapy agents for patients remain as an obstacle. New
drugs integrating immunotherapeutic and conventional cytotoxic effects is a powerful strategy for the treatment of
cancer to overcome this limitation. Antineoplastic phospholipids combine both of these activities by affecting lipid
metabolism and signaling through lipid rafts. Therefore, they emerge as interesting scaffolds for designing new drugs.

Objective:
We aimed to evaluate antineoplastic phospholipids as scaffolds for designing new drugs for lung
cancer treatment.

Methods:
The initial screening in A549 cells was performed by MTT assay. Others cytotoxic effects were
evaluated in A549 cells by clonogenic assay, Matrigel 3D culture and flow cytometry analyses of cell cycle,
apoptosis, mitochondrial membrane electronic potential and superoxide production. Immunological effects of
ED were accessed on dendritic cells (DCs) and the expression of some markers were evaluated by flow cytometry.
In vivo lung colonization analysis was performed after intravenously injection of A549 cells and daily
treatment with ED.

Results:
Herein, ED showed to be the most efficient compound concerning cytotoxic, thereby, ED was selected for
following tests. ED showed a cytotoxic profile in both monolayer and 3D culture and also in vivo models using
A549 cells. This profile is due to G0/G1 phase cellular arrest and apoptosis drove by mitochondrial membrane
depolarization and superoxide overproduction. Moreover, ED modulated DCs toward an activated pattern by the
increased expression of CD83 and a remarkable decreased expression of PD-L1/CD274 on DCs membrane.

Conclusions:
Thus, ED is an interesting antitumor drug prototype due to not only its direct cellular cytotoxicity
but also given its immunological features.
The Clinical and Experimental Research on the Treatment of Endometriosis with Thiostrepton
Authors: Jin Ping , Chen Xiaofei , Yu Guiyuan , Li Ziyang , Zhang Qingqing , Zhang Jian V. 
Published: January 2018

Background/Objective:Forkhead Box M1 (FOXM1) is frequently activated in tumors. We studied the expression and the possible mechanism of FOXM1 and evaluated the effects of thiostrepton in an endometriotic rat model.Methods and Material:This was a randomized study in a rat model of endometriosis. Fifty female Wistar rats were surgically induced with endometriosis. After 4 weeks of observation, twenty and thirty rats were randomly allocated to an ovariectomized (OVX) group and a treatment group, respectively. The OVX group was ovariectomized and randomly divided into an OVX-estrogen group and a control (OVX -oil) group. All rats were allowed a resting period of 3 days prior to any operation. The rats in the estrogen group were given estradiol (20 µg/kg, 0.1 ml /d), while the control group was treated with an equivalent amount of sesame oil. Every group was injected with subcutaneous injection for 7 days. The treatment group was randomly divided into three groups to receive the following: TST at 150 mg/kg, ip.; TST at 250 mg/kg, ip.; or sterile normal saline, ip. The groups received these dosages every 2 days for 2 weeks. Lesion growth, histological examination, and protein expression were subsequently analyzed using caliper measurement, histology, immunostaining, and Western blot after each rat received an injection in its own group.Results:Our results showed that FOXM1 is enriched in nucleus of an ectopic endometrium when compared with a eutopic uterus. Furthermore, we found that an ERK/FOXM1/matrix metalloproteinase-9 (MMP9) signaling pathway might result in the establishment and development of endometriosis. Finally, a thiostrepton concentration dependently reduced the expression of FOXM1, MMP9 and Bcl-2 in endometriotic lesions of the treated rats. Statistical significance was accepted for a value of P < 0.05.Conclusion:We postulate that thiostrepton could inhibit the endometriotic lesions, at least in part, by decreasing the FOXM1 expression and exerting a pro-apoptotic effect. We reported for the first time that FOXM1 expresses in experimental endometriosis rat and thiostrepton may also be suitable for the administration of endometriosis by inhibiting the growth of endometriotic implants. More studies are needed to further evaluate thiostrepton’s effect.
Morphological and Molecular Observation to Confirm the Taxonomic of Coptocercus biguttatus (Coleoptera: Cerambycidae) on Cloves in Ambon and Part of Ceram Island
Authors: Setyolaksono Mohamad Pamuji , Suputa Suputa , Putra Nugroho Susetya 
Published: January 2018

This research was conducted to confirm the species of longhorn beetle (Coptocercus biguttatus) drilling clove stems in Ambon and part of Ceram Island, Moluccas, which has been noted as an important pest. Aim of this investigation was to characterize the species morphologically, and more detailed with molecular technique via mtCO1 gene analysis. The longhorn beetle was taken in Ambon and part of Ceram Island, Moluccas and then was etablished in laboratory with host rearing method on pieces of clove stem. The results showed that C. biguttatus attacking clove stems in Ambon and part of Ceram Island, Maluku was closely related and grouped into same cluster with C. rubripes and P. semipunctata in of New Zealand with 85% homology value. C. biguttatus distributed evenly in all clove planting areas in Ambon and part of Ceram Island. IntisariPenelitian ini dilakukan untuk mengonfirmasi spesies kumbang sungut panjang (Coptocercus biguttatus) yang menggerek batang tanaman cengkih di Pulau Ambon dan sebagian dari Pulau Seram, Maluku menggunakan karakter morfologi dan molekuler berbasis gen mtCO1 untuk sidik kekerabatan. Pengambilan sampel kumbang sungut panjang dilakukan di Pulau Ambon dan sebagian dari Pulau Seram, Maluku dengan cara host rearing pada potongan batang cengkih. Hasil penelitian menunjukkan bahwa C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram, Maluku berkerabat dekat dan berada dalam satu kelompok dengan C. rubripes dan P. semipunctata di New Zealand dengan nilai homologi 85%. Penyebaran C. biguttatus di Pulau Ambon dan sebagian dari Pulau Seram merata di seluruh area pertanaman cengkih.
Characterization of Pantoea ananatis Isolated from Garlic and Shallot
Authors: Nurjanah Nanik , Joko Tri , Subandiyah Siti 
Published: January 2018

The new disease on garlic (Allium sativum) and shallot (A. cepa L. aggregatum group) have been found in several production centers of garlic and shallot in Tawangmangu and Temanggung, Central Java. The infected plants showed symptoms of leaf blight accompanied by chlorosis. The objective of this study was to determine the pathogen that causes leaf blight and chlorosis based on the phenotypic characterization and gyrB gene sequences analysis. The research started from the isolation of pathogen, physiological and biochemical test, DNA extraction, and sequence analysis of gyrB using gyrB 01-F and gyrB 02-R primer. The results showed that the isolated bacterial pathogen have a yellow pigment, slimy colonies with regular borders, convex, gram-negative, non-spore, facultative anaerobic, motile, catalase production, indole production, and acid production from D-glucose, D-mannitol, sucrose, and lactose. From the pathogenicity test, it was found that the bacteria produced the typical symptom of leaf blight. Characterization of pathogens based on gyrB gene sequence revealed that the pathogen was placed in the group of Pantoea ananatis. IntisariPenyakit baru pada bawang putih (Allium sativum) dan bawang merah (A. cepa L. aggregatum group) telah ditemukan di beberapa sentra produksi bawang putih dan bawang merah di Tawangmangu dan Temanggung, Jawa Tengah. Tanaman yang terinfeksi menunjukkan gejala hawar daun disertai klorosis. Tujuan penelitian untuk mengetahui karakter patogen berdasarkan fenotipik dan sekuen gen gyrB. Penelitian dimulai dengan isolasi bagian tanaman yang sakit, uji fisiologi dan biokimia, ekstraksi DNA dengan metode CTAB/NaCl dan amplifikasi gen gyrB menggunakan primer gyrB 01-F and gyrB 02-R. Hasil uji menunjukkan koloni berlendir, cembung, pigmen berwarna kuning, gram negative, tidak berspora, aerob fakultatif, motil, produksi katalase, indol, membentuk asam dari D-glukosa, D-monnitol, sukrosa dan laktosa, dan patogenesitas positif. Karakterisasi patogen berdasarkan sekuen gen gyrB, menunjukkan patogen hawar daun berkerabat dekat dengan Pantoea ananatis.
Interaksi Meloidogyne incognita dan Fusarium solani pada Penyakit Kuning Lada
Authors: Suryanti Suryanti , Hadisutrisno Bambang , Mulyadi Mulyadi , Widada Jaka 
Published: January 2018

Pepper yellowing disease is one of the most important disease of pepper causing the decrease of pepper production. This research was conducted in the screen house and laboratory to determine the major causal agent of leaf yellowing disease of pepper. Meloidogyne incognita and Fusarium solani were isolated from pepper plantation in West Kalimantan. Pepper seedlings Natar 1 cultivars were planted in sterilized soil collected from pepper plantation in Bengkayang, West Kalimantan. Five months-old seedling were inoculated with M. incognita (1000 larvae of 2nd stadium/plant) and F. solani (50 ml of spore suspension with spore density of 106 spore/ml) in several combinations of time inoculation, i.e., F. solani and then M. incognita, M. incognita and then F. solani, M. incognita together with F. solani, M. incognita only, and F. solani only. The parameters observed were the development of leaf yellowing disease every weeks for five months. The number of gall, and population M. incognita were observed at the end of the observation. The result showed that when M. incognita was inoculated to the roots followed by F. solani, the disease severity and the percentage of plant diseases were higher than those which were infected with F. solani or M. incognita alone. The higher population densities of M. incognita and a number of root gall, had observed on plants inoculated by M. incognita combined with F. solani than plants inoculated by M. incognita and F. solani alone. Interaction between M. incognita and F. solani as caused of leaf yellowing disease of pepper was synergistic reaction. IntisariPenyakit kuning lada merupakan salah satu penyakit penting pada lada yang mengakibatkan terjadinya penurunan produksi lada di Indonesia. Penelitian ini bertujuan untuk mengetahui peran Meloidogyne incognita dan Fusarium solani sebagai penyebab penyakit kuning lada. Penelitian yang dilakukan rumah kasa dan laboratorium. Meloidogyne incognita dan Fusarium solani diisolasi dari pertanaman lada di Kalimantan Barat. Penelitian dilakukan dengan menggunakan bibit lada kultivar Natar 1 berumur 5 bulan, dan diinokulasi dengan M. incognita sebanyak 1000 larva stadium 2 dan 50 ml suspensi mikrokonidium F. solani dengan kerapatan 106/ml. Perkembangan gejala penyakit diamati setiap minggu selama 5 bulan, dan pada akhir pengamatan dilakukan penghitungan jumlah puru dan populasi M. incognita. Hasil penelitian menunjukkan bahwa apabila M. incognita menginfeksi akar dan selanjutnya diikuti dengan infeksi oleh F. solani, tingkat keparahan penyakit dan persentase tanaman sakit lebih tinggi dibandingkan dengan infeksi oleh F. solani atau M. incognita secara terpisah. Populasi M. incognita dan jumlah puru akar pada tanaman yang diinokulasi dengan M. incognita bersama-sama dengan F. solani lebih tinggi dibandingkan pada tanaman yang diinokulasi dengan M. incognita atau F. solani secara terpisah. Interaksi antara M. incognita dan F. solani dalam menyebabkan penyakit kuning lada adalah bersifat sinergis.
BIBLIOMETRIC ANALYSIS OF CARBON DIRECTION IN NANOTECHNOLOGY: 2000–2015
Authors: Terekhov A. I. 
Published: January 2018

Carbon nanostructures are the important component of nanotechnology (NT) become as the first global scientific and technological initiative of the 21st century. The article presents a bibliometric analysis of development of the carbon direction of NT in the period of 2000–2015, including an international dimension. The source of information was the Science Citation Index Expanded database. The shift of the world research center to the Asian region is shown, both in terms of volume and quality indicators; the dynamics of scientific influence of two groups of countries – conditionally «incumbents» and «newcomers» – has been studied. Due to the strong skewness of citation distributions, preference in the analysis is given to the percentile-based indicators, such as: the contribution of a country to the world top-10% (top-1%) segment of the most highly cited publications, the share of such publications in the country’s total output, the highly cited papers index, etc. Relying on them, the scientific «offensive» of the «newcomer» countries on the «incumbents» (eg, of China on the USA, of South Korea on Germany, of Iran on Russia) as well as phenomenon of Singapore as an effective producer of highly cited publications on the carbon nanostructures were presented more fully. The positions of Russia have been studied in more detail, the main domestic research participants have been determined, and on the basis of bibliometric criteria the center of scientific excellence in the field of graphene has been identified.
Stability Studies of Immobilized Saccharomyces Cerevisiae in Calcium Alginate and Carrageenan Beads
Authors: Abdulla Rahmath , Ajak Warda Abdul , Hajar Siti , Derman Eryati 
Published: January 2018

Currently the resources for fossil fuels are depleting together with increase in fuel prices. This has urged the need for cheaper alternative fuels especially biofuels. The production of the most common liquid biofuel which is bioethanol using immobilized yeast cells is an approach taken to increase its demand in the world’s market. There are various methods for the immobilization of yeast cells; however before they can be applied in the industry the stability of the immobilization technology must be investigated. This research aims to study the stabilities of immobilized S. cerevisiae in calcium alginate and carrageenan beads for bioethanol production. The S. cerevisiae was immobilized in calcium alginate and carrageenan beads using entrapment method. Next, screening for the optimal concentration of sodium alginate and semi refined carrageenan matrices were determined by employing fermentation and bioethanol quantification using GC-MS. Concentrations of 2% (w/v) calcium alginate and 2% (w/v) semi refined carrageenan beads were identified to produce the highest bioethanol yield which were 0.286 g/mL and 0.065 g/mL respectively. The two beads were then chosen to be tested in various stability studies with respect to bioethanol production such as storage stability, reusability, pH, thermaland permeability test. It was found out that a concentration of 2% (w/v) calcium alginate beads were more stable as immobilization matrix for S. cerevisiae as compared to 2% (w/v) semi refined carrageenan.
Aristóteles y la democracia
Authors: Solana Dueso José 
Published: January 2018

El objeto de este texto es abordar algunos aspectos de las ideas políticas de Aristóteles y, en particular, de su actitud y de sus argumentos sobre la democracia. A diferencia de Jaeger, el trabajo se desarrolla desde el supuesto de que el pensamiento de Aristóteles se configura en un proceso de tensión constante, y no necesariamente lineal, entre los dos grandes pensadores que le precedieron: Protágoras y Platón. Esta doble tensión hace emerger todo un entramado teórico, relacionado sobre todo con la filosofía práctica, pero con profundas raíces en la filosofía teórica, en el que Aristóteles se alinea con las tesis protagóricas. Una de ellas es la defensa del sistema democrático. El artículo muestra, no solo que Aristóteles es partidario de la democracia como mejor forma de constitución, sino que solo la democracia satisface las condiciones de una autoridad política, por el hecho de que es la justicia, virtud moral por antonomasia, la que exige ejercer el mando por turno y que todos los ciudadanos manden en un momento y obedezcan en otro. TITLE: Aristotle and Democracy ABSTRACTThe purpose of this paper is to address some aspects of Aristotle's political ideas and, in particular, his attitude and his arguments about democracy. Unlike Jaeger's approach, the paper starts from the assumption that Aristotle's thinking is shaped by a process of constant, and not necessarily linear, tension between the two great thinkers who preceded him: not only Plato but also Protagoras. This dual tension brings to the surface a theoretical framework, related above all to practical philosophy, but with deep roots in theoretical philosophy, in which Aristotle is aligned with theses of Protagoras. One of them is the defense of the democratic system. The article shows, not only that Aristotle favors democracy as the best form of constitution, but that only democracy satisfies the conditions of a political authority, because it is justice, the moral virtue par excellence, which demands to exercise the command by turn and that all the citizens command in one moment and obey in another.
РУХОВА РЕАБІЛІТАЦІЯ ХВОРИХ ПІСЛЯ ОПЕРАЦІЇ З ПРИВОДУ ПІСЛЯТРАВМАТИЧНОЇ КОНТРАКТУРИ ЛІКТЬОВОГО СУГЛОБА
Authors: Кurinniy I. M. , Strafun О. S. , Dolgopolov О. V. , Gerasymenko А. S. 
Published: January 2018

Вступ. Перелом в ділянці ліктьового суглоба (ЛС) становить 5 - 6% в структурі всіх ушкоджень скелета, проте, частота незадовільних результатів лікування та утворення контрактури цієї зони сягає 20 - 25%.
Матеріали і методи. Порівнювали ефективність розробки рухів під час реабілітації хворих, оперованих з приводу післятравматичної контрактури ЛС. Ретроспективний аналіз проведений у 69 пацієнтів з згинально-розгинальною контрактурою ЛС, яким виконували операцію артролізу. У пацієнтів 1-ї групи застосовували активну реабілітаційну програму без використання фіксуючих пристроїв; 2-ї групи – під час розробки рухів застосовували дві гіпсові шини у положенні максимального згинання та розгинання мобілізованого ЛС.
Результати. Після оперативного втручання та рухової реабілітації кінцевий обсяг згинально-розгинальних рухів у пацієнтів 1-ї групи збільшився на 59,58º, 2-ї групи - на 70,7º. За шкалою Mayo Elbow Performance Score (MEPS), через 6 міс після операції стан пацієнтів 1-ї групи оцінений у середньому (75 ± 7,3) бала, 2-ї групи (84,6 ± 10,5) бала.
Висновок. Лікування післятравматичної контрактури ЛС є вкрай складною, актуальною проблемою, потребує індивідуального підходу.
При порівнянні ефективності реабілітації пацієнтів відзначене достовірне збільшення обсягу рухів та кращий функціональний результат за MEPS при використанні двох змінних гіпсових шин.
Ключові слова: ліктьовий суглоб; післятравматична контрактура; рухова реабілітація.
ЕКСПЕРИМЕНТАЛЬНА МОДЕЛЬ ЛІКУВАННЯ ХРОНІЧНОЇ КРИТИЧНОЇ ІШЕМІЇ ТКАНИН НИЖНЬОЇ КІНЦІВКИ З ВИКОРИСТАННЯМ КУЛЬТУРИ СТОВБУРОВИХ КЛІТИН, ОДЕРЖАНИХ З ЖИРОВОЇ ТКАНИНИ
Authors: Litvinova N. Yu. , Міshalov V. G. , Chernyak V. А. , Dubenko D. E. , Likhodiyevskyi V. V. , Коrsak А. V. , Zubov D. О. , Vasilyev R. G. 
Published: January 2018

Вступ. Захворювання периферійних артерій (ЗПА) є важливою медико-соціальною проблемою, що потребує розробки нових видів терапії, зокрема, використання аутологічних стовбурових клітин.
Мета. Вивчити морфофункціональний стан нижніх кінцівок кролів, у яких моделювали хронічну критичну ішемію тканин нижніх кінцівок (ХКІНК) та застосовували культуру аутологічних стовбурових клітин, одержаних з їх жирової тканини (ADSCs).
Матеріали і методи. У дослідження включені 18 кролів-самиць, розподілених на 3 групи: псевдооперованих (І), оперованих, яким вводили ізотонічний розчин NaCl (ІІ), оперованих, яким вводили ADSCs (ІІІ). Морфофункціональний стан тазових кінцвок оцінювали за даними дуплексної флоуметрії, функціональних тестів та морфологічного дослідження.
Результати. У тварин ІІІ групи спостерігали часткове відновлення функції тазової кінцівки та покращення стану м’язової тканини, чого не було у тварин ІІ групи. В ІІІ групі відзначене припинення прогресування некротичних процесів та часткова епітелізація трофічних виразок.
Висновки. Використання ADSCs при моделюванні ХКІНК у кролів сприяло відновленню функції тазової кінцівки, що проявлялося якісними й кількісними змінами морфофункціональних показників через 5 тиж після введення стовбурових клітин.
Ключові слова: хронічна критична ішемія тканин нижніх кінцівок; захворювання периферійних артерій; ADSCs; експеримент.