bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experiences of two multidisciplinary team members of systemic consultations in a community learning disability service
Authors: Johnson Clair , Viljoen Nina 
Published: June 2017

Accessible Summary

People with learning disabilities may have help from their families, paid carers and professionals such as nurses, psychologists etc. Professionals (like psychologists) can help people with learning disabilities and their families, carers and other professionals by meeting to talk about solutions together. This is called a systemic consultation.

We asked two people who came to the systemic consultations what they thought was helpful and unhelpful about the systemic consultations. The two people we asked were two professionals who worked with people with learning disabilities.

The helpful things were that the systemic consultations might have helped people to feel better about themselves and speak about their worries. People may feel that others care about them too. People with learning disabilities started to find their own answers to the difficulties they had. We found that the people who were responsible for the systemic consultations asked some helpful questions. For example, they spoke in a positive way and tried to talk about the things that people were good at. Another helpful thing was that coming to the systemic consultations helped people to get on better with each other.

The unhelpful things were that people coming to the systemic consultations thought that something else would happen at the consultations. They also thought that sometimes there were too many people at the systemic consultations. They said that it was not helpful if the people who help in the systemic consultations change.

After looking at the answers the people gave, we talked about the things that can be done to make sure that systemic consultations become more helpful for everyone.

AbstractBackgroundSystemic approaches can be useful in working with people with learning disabilities and their network. The evidence base for these approaches within the field of learning disabilities, however, is currently limited.Materials and MethodsThis article presents part of a service evaluation of systemic consultations in a Community Learning Disability Service (CLDS). Two multidisciplinary team professionals were interviewed about what they found helpful and unhelpful in their experiences of attending systemic consultations. Interview data were analysed via applying thematic analysis principles.ResultsFive main themes were elicited: differing expectations, enabling for the client, positive techniques, uncertainty regarding “forum” (“forum” meaning the collective of all of the individuals meet, which could include the professionals, lead therapist, reflecting team, etc.) composition and strengthening the network.ConclusionsSystemic consultations provided some positive and valuable experiences along with important caveats. Considerations for clinical practice are therefore discussed. Continued evaluation of systemic consultations within CLDSs is recommended.
Identification of lateralized compensatory neural activities within the social brain due to autism spectrum disorder in adolescent males
Authors: Subbaraju Vigneshwaran , Sundaram Suresh , Narasimhan Sundararajan 
Published: June 2017

AbstractSocio‐behavioral impairments are important characteristics of autism spectrum disorders (ASD) and MRI‐based studies are pursued to identify a neurobiological basis behind these conditions. This paper presents an MRI‐based study undertaken to (i) identify the differences in brain activities due to ASD, (ii) verify whether such differences exist within the ‘social brain’ circuit which is hypothesized to be responsible for social functions, and (iii) uncover potential compensatory mechanisms within the identified differences in brain activities. In this study, a whole‐brain voxel‐wise analysis is performed using resting‐state fMRI data from 598 adolescent males, that is openly available from the ABIDE consortium. A new method is developed, which can (i) extract the discriminative brain activities, that provide high separability between the blood oxygenation time‐series signals from ASD and neurotypical populations, (ii) select the activities that are relevant to ASD by evaluating the correlation between the separability and traditional severity scores, and (iii) map the spatial pattern of regions responsible for generating the discriminative activities. The results show that the most discriminative brain activities occur within a subset of the social brain that is involved with affective aspects of social processing, thereby supporting the idea of the social brain and also its fractionalization in ASD. Further, it has also been found that the diminished activities in the posterior cingulate area are potentially compensated by enhanced activities in the ventromedial prefrontal and anterior temporal areas within the social brain. Hemispherical lateralization is also observed on such compensatory activities.
Climate change vulnerability for species—Assessing the assessments
Authors: Wheatley Christopher J. , Beale Colin M. , Bradbury Richard B. , Pearce‐Higgins James W. , Critchlow Rob , Thomas Chris D. 
Published: June 2017

AbstractClimate change vulnerability assessments are commonly used to identify species at risk from global climate change, but the wide range of methodologies available makes it difficult for end users, such as conservation practitioners or policymakers, to decide which method to use as a basis for decision‐making. In this study, we evaluate whether different assessments consistently assign species to the same risk categories and whether any of the existing methodologies perform well at identifying climate‐threatened species. We compare the outputs of 12 climate change vulnerability assessment methodologies, using both real and simulated species, and validate the methods using historic data for British birds and butterflies (i.e. using historical data to assign risks and more recent data for validation). Our results show that the different vulnerability assessment methods are not consistent with one another; different risk categories are assigned for both the real and simulated sets of species. Validation of the different vulnerability assessments suggests that methods incorporating historic trend data into the assessment perform best at predicting distribution trends in subsequent time periods. This study demonstrates that climate change vulnerability assessments should not be used interchangeably due to the poor overall agreement between methods when considering the same species. The results of our validation provide more support for the use of trend‐based rather than purely trait‐based approaches, although further validation will be required as data become available.
Hepatocellular malignant neoplasm, NOS: a clinicopathological study of 11 cases from a single institution
Authors: Zhou Shengmei , Venkatramani Rajkumar , Gupta Shveta , Wang Kasper , Stein James E , Wang Larry , Mascarenhas Leo 
Published: June 2017

AimsThe primary aim of this study is to characterize hepatocellular malignant neoplasm, NOS (HEMNOS), a new provisional entity describing a subset of paediatric hepatocellular tumours, which have histological features of neither typical hepatoblastoma (HB) nor hepatocellular carcinoma (HCC).Methods and resultsThe clinicopathological features of 11 patients with HEMNOS were analysed retrospectively. The median age and serum alpha‐fetoprotein level at diagnosis was 7 years and 182 000 ng/ml, respectively. Ten patients presented with pretreatment extent of disease (PRETEXT) stages III/IV multifocal tumours, eight with major vascular involvement, three with lung metastases and three with extrahepatic extension. The original pathology diagnoses were: HB in seven patients, HCC in two and HEMNOS in two. Our pathology review of pre‐chemotherapy specimens showed that six tumours had equivocal/overlapping histological features of HB and HCC, four had predominant HB histology along with focal HCC‐like histology and one had HB histology. Seven of nine post‐chemotherapy resection specimens showed predominant HCC‐like histology. Beta‐catenin, glypican 3 and spalt‐like transcription factor 4 immunostaining showed that all the tumours had a mixed HB/HCC immunophenotype. Telomerase reverse transcriptase immunostaining showed nuclear staining in nine of the 11 tumours. All patients received chemotherapy and achieved gross total primary tumour resection. Nine of the 11 patients were treated with established HB chemotherapy regimens. After a median follow‐up of 6.1 years (range: 1.2–11.8 years), all patients were in remission.ConclusionsHEMNOS is a subtype of HB with focal HCC‐like histology, a high‐risk clinical profile but favourable outcome following chemotherapy and complete tumour resection.
From intention to STI prevention: An online questionnaire on barriers and facilitators for discussing sexual risk behaviour among HIV nurses
Authors: de Munnik Suzanne , Vervoort Sigrid C.J.M. , Ammerlaan Heidi S.M. , Kok Gerjo , den Daas Chantal 
Published: June 2017

AbstractAimsWe aimed to elucidate facilitators and barriers that HIV nurses experience in discussing sexual risk behaviour with HIV‐positive men who have sex with men, using variables from a previous qualitative study and the theory of planned behaviour.BackgroundHIV‐positive men who have sex with men are frequently diagnosed with sexually transmitted infections, which can be reduced if HIV nurses discuss sexual risk behaviour.DesignAn online questionnaire was disseminated in April 2015 among all HIV nurses in the Netherlands.MethodsWe assessed variables, such as attitudes, shame, ability, knowledge and time concerns. A regression analysis was conducted with “intention to discuss sexual risk behaviour” as an outcome variable.ResultsThe questionnaire was completed by 60 of 79 HIV nurses. Overall, participants reported high intentions to discuss sexual risk behaviour, and 38% of the variance was explained by attitude, sexual preference, knowing ways to introduce the topic and experiencing enough time or viewing it as a priority. In addition, high intenders significantly differed from low intenders in “experienced shame,” “relation with patients,” “non‐verbal communication,” “subjective norm” and “knowledge.”ConclusionImproving sexual health in HIV care translates into improving opportunities and the facilitating factors in initiating the discussion of sexual risk behaviour rather than removing barriers HIV nurses experience. Interventions should mainly focus on improving the HIV nurses’ perceived ability to initiate the topic of sexual risk behaviour and to utilize the jargon and terminology that is commonly used among men who have sex with men.
Regulatory mode and willingness to increase retirement savings contributions
Authors: Kim Hyungsoo , Shin Serah , Heath Claudia J. , Zhang Qun , Higgins E. Tory 
Published: June 2017

AbstractThis study examined how two self‐regulatory modes, locomotion and assessment, relate to the willingness to increase retirement savings. Locomotion is concerned with making things happen (“just do it”). Assessment is concerned with critical evaluation (“do the right thing”). We hypothesized that individuals who score high (vs. low) in locomotion, but not those who score high (vs. low) in assessment, would be more willing to increase their savings for retirement. In addition, because high (vs. low) assessment can lead to doing the right thing in terms of seeking to maximize economic returns, we hypothesized that combining high locomotion with high assessment would especially motivate willingness to increase retirement savings. We found support for both hypotheses from a survey of university employees.
Rho‐kinase inhibitor Y‐27632 facilitates the proliferation, migration and pluripotency of human periodontal ligament stem cells
Authors: Wang Ting , Kang Wenyan , Du Lingqian , Ge Shaohua 
Published: June 2017

AbstractThe selective in vitro expansion and differentiation of multipotent stem cells are critical steps in cell‐based regenerative therapies, while technical challenges have limited cell yield and thus affected the success of these potential treatments. The Rho GTPases and downstream Rho kinases are central regulators of cytoskeletal dynamics during cell cycle and determine the balance between stem cells self‐renewal, lineage commitment and apoptosis. Trans‐4‐[(1R)‐aminoethyl]‐N‐(4‐pyridinyl)cylohexanecarboxamidedihydrochloride (Y‐27632), Rho‐associated kinase (ROCK) inhibitor, involves various cellular functions that include actin cytoskeleton organization, cell adhesion, cell motility and anti‐apoptosis. Here, human periodontal ligament stem cells (PDLSCs) were isolated by limiting dilution method. Cell counting kit‐8 (CCK8), 5‐ethynyl‐2′‐deoxyuridine (EdU) labelling assay, cell apoptosis assay, cell migration assay, wound‐healing assay, alkaline phosphatase (ALP) activity assay, Alizarin Red S staining, Oil Red O staining, quantitative real‐time polymerase chain reaction (qRT‐PCR) were used to determine the effects of Y‐27632 on the proliferation, apoptosis, migration, stemness, osteogenic and adipogenic differentiation of PDLSCs. Afterwards, Western blot analysis was performed to elucidate the mechanism of cell proliferation. The results indicated that Y‐27632 significantly promoted cell proliferation, chemotaxis, wound healing, fat droplets formation and pluripotency, while inhibited ALP activity and mineral deposition. Furthermore, Y‐27632 induced PDLSCs proliferation through extracellular‐signal‐regulated kinase (ERK) signalling cascade. Therefore, control of Rho‐kinase activity may enhance the efficiency of stem cell‐based treatments for periodontal diseases and the strategy may have the potential to promote periodontal tissue regeneration by facilitating the chemotaxis of PDLSCs to the injured site, and then enhancing the proliferation of these cells and maintaining their pluripotency.
Up‐regulated basigin‐2 in microglia induced by hypoxia promotes retinal angiogenesis
Authors: Yin Jie , Xu Wen‐Qin , Ye Ming‐Xiang , Zhang Yong , Wang Hai‐Yan , Zhang Jian , Li Yu , Wang Yu‐Sheng 
Published: June 2017

AbstractRetinal microglia cells contribute to vascular angiogenesis and vasculopathy induced by relative hypoxia. However, its concrete molecular mechanisms in shaping retinal angiogenesis have not been elucidated. Basigin, being involved in tumour neovasculogenesis, is explored to exert positive effects on retinal angiogenesis induced by microglia. Therefore, we set out to investigate the expression of basigin using a well‐characterized mouse model of oxygen‐induced retinopathy, which recapitulated hypoxia‐induced aberrant neovessel growth. Our results elucidate that basigin is overexpressed in microglia, which accumulating in retinal angiogenic sprouts. In vitro, conditioned media from microglia BV2 under hypoxia treatment increase migration and tube formation of retinal capillary endothelia cells, compared with media from normoxic condition. The angiogenic capacity of BV2 is inhibited after basigin knockdown by small interfering RNAs. A new molecular mechanism for high angiogenic capacity, whereby microglia cells release basigin via up‐regulation of PI3K‐AKT and IGF‐1 pathway to induce angiogenesis is unveiled. Collectively, our results demonstrate that basigin from hypoxic microglia plays a pivotal pro‐angiogenic role, providing new insights into microglia‐promoting retinal angiogenesis.
AEG‐1 induces gastric cancer metastasis by upregulation of eIF4E expression
Authors: Wu Shengjie , Yang Li , Wu Dandan , Gao Zhongyuan , Li Ping , Huang Wenbin , Wang Xuerong 
Published: June 2017

AbstractGastric cancer is the third leading cause of cancer‐related deaths worldwide, and patients with lymph node, peritoneal and distant metastasis have a poor prognosis. Overexpression of Astrocyte‐elevated gene‐1 (AEG‐1) has been reported to be correlated with the progression and metastasis of gastric cancer. However, its mechanisms are quite unclear. In this study, we found that elevated expression of AEG‐1 was correlated with metastasis in human gastric cancer tissues. Moreover, gain‐ or loss‐of‐function of AEG‐1, respectively, promoted or suppressed epithelial–mesenchymal transition (EMT), migration and invasion of gastric cancer cells. AEG‐1 positively regulated eIF4E, MMP‐9 and Twist expression. Manipulating eIF4E expression by transfection of overexpression constructs or siRNAs partially eliminated AEG‐1‐regulated EMT, cell migration and invasion. In addition, overexpression or knockdown of eIF4E promoted or suppressed EMT, cell migration and invasion in parallel with upregulation of MMP‐9 and Twist expression, while manipulating eIF4E expression partially abrogated AEG‐1‐induced MMP‐9 and Twist. Finally, silencing of AEG‐1 expression not only inhibited tumour growth in parallel with downregulation of eIF4E, MMP‐9 and Twist expression in a xenograft nude mouse model, but also suppressed lymph node and peritoneal metastasis of gastric cancer in an orthotopic nude mouse model. These findings suggest that AEG‐1 promotes gastric cancer metastasis through upregulation of eIF4E‐mediated MMP‐9 and Twist, which provides new diagnostic markers and therapeutic targets for cancer metastasis.
Gene‐based interaction analysis shows GABAergic genes interacting with parenting in adolescent depressive symptoms
Authors: Van Assche Evelien , Moons Tim , Cinar Ozan , Viechtbauer Wolfgang , Oldehinkel Albertine J. , Van Leeuwen Karla , Verschueren Karine , Colpin Hilde , Lambrechts Diether , Van den Noortgate Wim , Goossens Luc , Claes Stephan , van Winkel Ruud 
Published: June 2017

BackgroundMost gene‐environment interaction studies (G × E) have focused on single candidate genes. This approach is criticized for its expectations of large effect sizes and occurrence of spurious results. We describe an approach that accounts for the polygenic nature of most psychiatric phenotypes and reduces the risk of false‐positive findings. We apply this method focusing on the role of perceived parental support, psychological control, and harsh punishment in depressive symptoms in adolescence.MethodsAnalyses were conducted on 982 adolescents of Caucasian origin (Mage (SD) = 13.78 (.94) years) genotyped for 4,947 SNPs in 263 genes, selected based on a literature survey. The Leuven Adolescent Perceived Parenting Scale (LAPPS) and the Parental Behavior Scale (PBS) were used to assess perceived parental psychological control, harsh punishment, and support. The Center for Epidemiologic Studies Depression Scale (CES‐D) was the outcome. We used gene‐based testing taking into account linkage disequilibrium to identify genes containing SNPs exhibiting an interaction with environmental factors yielding a p‐value per single gene. Significant results at the corrected p‐value of p < 1.90 × 10−4 were examined in an independent replication sample of Dutch adolescents (N = 1354).ResultsTwo genes showed evidence for interaction with perceived support: GABRR1 (p = 4.62 × 10−5) and GABRR2 (p = 9.05 × 10−6). No genes interacted significantly with psychological control or harsh punishment. Gene‐based analysis was unable to confirm the interaction of GABRR1 or GABRR2 with support in the replication sample. However, for GABRR2, but not GABRR1, the correlation of the estimates between the two datasets was significant (r (46) = .32; p = .027) and a gene‐based analysis of the combined datasets supported GABRR2 × support interaction (p = 1.63 × 10−4).ConclusionsWe present a gene‐based method for gene–environment interactions in a polygenic context and show that genes interact differently with particular aspects of parenting. This accentuates the importance of polygenic approaches and the need to accurately assess environmental exposure in G × E.
Advanced film‐forming gel formula vs spring thermal water and white petrolatum as primary dressings after full‐face ablative fractional CO2 laser resurfacing: a comparative split‐face pilot study
Authors: Marini L. 
Published: June 2017

AbstractBackgroundAesthetically pleasing results and fast, uneventful recovery are highly desirable after rejuvenating ablative laser procedures. Wound dressings following ablative laser procedures should ideally improve and optimize the wound healing environment.ObjectiveThe purpose of this comparative split‐face, single‐blinded, prospective observational study was to assess the efficacy and acceptability of two primary wound dressings immediately after a full‐face fractional CO2 laser resurfacing procedure.MethodsThe assessments of an innovative film‐forming dressing called Stratacel (SC) vs spring thermal water + Vaseline (V+) were conducted after a standardized, single‐pass, full‐face ablative fractional CO2 laser skin resurfacing procedure. Clinical parameters, such as haemoglobin – HB; surface temperature – ST; micro‐textural modifications – MT; superficial melanin – M; intrafollicular porphyrins – P, were assessed at different phases of the healing process using standardized, non‐invasive technologies.ResultsFive female volunteers were enrolled in this inpatient, controlled pilot study. Most of the clinical parameters considered, including 3D surface texture analysis, revealed a better performance of SC vs. V+ during the early, more delicate phases of the healing process.ConclusionsThis preliminary study, even if performed on a small number of volunteers, confirmed a definite advantage of the tested semipermeable film‐forming formula (SC) over a more conventional postoperative skin care regime (V+). Clinical results could be explained by a better uniformity of distribution of SC over the micro‐irregularities induced by ablative fractional CO2 laser resurfacing. Its thin, semipermeable film might, in fact, act as an efficient, perfectly biocompatible, full contact, temporary skin barrier, able to protect extremely delicate healing surfaces from potential environmental irritations.
Clinical and genetic diversities of Charcot‐Marie‐Tooth disease with MFN2 mutations in a large case study
Authors: Ando Masahiro , Hashiguchi Akihiro , Okamoto Yuji , Yoshimura Akiko , Hiramatsu Yu , Yuan Junhui , Higuchi Yujiro , Mitsui Jun , Ishiura Hiroyuki , Umemura Ayako , Maruyama Koichi , Matsushige Takeshi , Morishita Shinichi , Nakagawa Masanori , Tsuji Shoji , Takashima Hiroshi 
Published: June 2017

AbstractCharcot‐Marie‐Tooth disease (CMT) constitutes a heterogeneous group affecting motor and sensory neurons in the peripheral nervous system. MFN2 mutations are the most common cause of axonal CMT. We describe the clinical and mutational spectra of CMT patients harboring MFN2 mutations in Japan. We analyzed 1,334 unrelated patients with clinically suspected CMT referred by neurological and neuropediatric departments throughout Japan. We conducted mutation screening using a DNA microarray, targeted resequencing, and whole‐exome sequencing. We identified pathogenic or likely pathogenic MFN2 variants from 79 CMT patients, comprising 44 heterozygous and 1 compound heterozygous variants. A total of 15 novel variants were detected. An autosomal dominant family history was determined in 43 cases, and the remaining 36 cases were reported as sporadic with no family history. The mean onset age of CMT in these patients was 12 ± 14 (range 0–59) years. We observed neuropathic symptoms in all patients. Some had optic atrophy, vocal cord paralysis, or spasticity. We detected a compound heterozygous MFN2 mutation in a patient with a severe phenotype and the co‐occurrence of MFN2 and PMP22 mutations in a patient with an uncommon phenotype. MFN2 is the most frequent causative gene of CMT2 in Japan. We present 15 novel variants and broad clinical and mutational spectra of Japanese MFN2‐related CMT patients. Regardless of the onset age and inheritance pattern, MFN2 gene analysis should be performed. Combinations of causative genes should be considered to explain the phenotypic diversity.