bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of strain relaxation in Pr0.67Sr0.33MnO3 films probed by polarization dependent X-ray absorption near edge structure
Authors: Zhang Bangmin , Chen Jingsheng , Yang Ping , Chi Xiao , Lin Weinan , Venkatesan T. , Sun Cheng-Jun , Heald Steve M. , Chow Gan Moog 
Published: January 2016

AbstractThe Mn K edge X-ray absorption near edge structure (XANES) of Pr0.67Sr0.33MnO3 films with different thicknesses on (001) LaAlO3 substrate was measured and the effects of strain relaxation on film properties were investigated. The films showed in-plane compressive and out-of-plane tensile strains. Strain relaxation occurred with increasing film thickness, affecting both lattice constant and MnO6 octahedral rotation. In polarization dependent XANES measurements using in-plane (parallel) and out-of-plane (perpendicular) geometries, the different values of absorption resonance energy Er confirmed the film anisotropy. The values of Er along these two directions shifted towards each other with increasing film thickness. Correlating with X-ray diffraction (XRD) results it is suggested that the strain relaxation decreased the local anisotropy and corresponding probability of electronic charge transfer between Mn 3d and O 2p orbitals along the in-plane and out-of-plane directions. The XANES results were used to explain the film-thickness dependent magnetic and transport properties.
Ultrafast strong-field photoelectron emission from biased metal surfaces: exact solution to time-dependent Schrödinger Equation
Authors: Zhang Peng , Lau Y. Y. 
Published: January 2016

AbstractLaser-driven ultrafast electron emission offers the possibility of manipulation and control of coherent electron motion in ultrashort spatiotemporal scales. Here, an analytical solution is constructed for the highly nonlinear electron emission from a dc biased metal surface illuminated by a single frequency laser, by solving the time-dependent Schrödinger equation exactly. The solution is valid for arbitrary combinations of dc electric field, laser electric field, laser frequency, metal work function and Fermi level. Various emission mechanisms, such as multiphoton absorption or emission, optical or dc field emission, are all included in this single formulation. The transition between different emission processes is analyzed in detail. The time-dependent emission current reveals that intense current modulation may be possible even with a low intensity laser, by merely increasing the applied dc bias. The results provide insights into the electron pulse generation and manipulation for many novel applications based on ultrafast laser-induced electron emission.
Continuous particle separation using pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE)
Authors: Jeon Hyungkook , Kim Youngkyu , Lim Geunbae 
Published: January 2016

AbstractIn this paper, we introduce pressure-driven flow-induced miniaturizing free-flow electrophoresis (PDF-induced μ-FFE), a novel continuous separation method. In our separation system, the external flow and electric field are applied to particles, such that particle movement is affected by pressure-driven flow, electroosmosis and electrophoresis. We then analyzed the hydrodynamic drag force and electrophoretic force applied to the particles in opposite directions. Based on this analysis, micro- and nano-sized particles were separated according to their electrophoretic mobilities with high separation efficiency. Because the separation can be achieved in a simple T-shaped microchannel, without the use of internal electrodes, it offers the advantages of low-cost, simple device fabrication and bubble-free operation, compared with conventional μ-FFE methods. Therefore, we expect the proposed separation method to have a wide range of filtering/separation applications in biochemical analysis.
Wind-blown Sand Electrification Inspired Triboelectric Energy Harvesting Based on Homogeneous Inorganic Materials Contact: A Theoretical Study and Prediction
Authors: Hu Wenwen , Wu Weiwei , Zhou Hao-miao 
Published: January 2016

AbstractTriboelectric nanogenerator (TENG) based on contact electrification between heterogeneous materials has been widely studied. Inspired from wind-blown sand electrification, we design a novel kind of TENG based on size dependent electrification using homogeneous inorganic materials. Based on the asymmetric contact theory between homogeneous material surfaces, a calculation of surface charge density has been carried out. Furthermore, the theoretical output of homogeneous material based TENG has been simulated. Therefore, this work may pave the way of fabricating TENG without the limitation of static sequence.
Cross-protection induced by Japanese encephalitis vaccines against different genotypes of Dengue viruses in mice
Authors: Li Jieqiong , Gao Na , Fan Dongying , Chen Hui , Sheng Ziyang , Fu Shihong , Liang Guodong , An Jing 
Published: January 2016

AbstractDengue viruses (DENVs) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses that cause very high global disease burdens. Although cross-reactivity and cross-protection within flaviviruses have been demonstrated, the effect of JEV vaccination on susceptibility to DENV infection has not been well elucidated. In this study, we found that vaccination with the JEV inactivated vaccine (INV) and live attenuated vaccine (LAV) could induce cross-immune responses and cross-protection against DENV1-4 in mice. Despite the theoretical risk of immune enhancement, no increased mortality was observed in our mouse model. Additionally, low but consistently detectable cross-neutralizing antibodies against DENV2 and DENV3 were also observed in the sera of JEV vaccine-immunized human donors. The results suggested that both JEV-LAV and JEV-INV could elicit strong cross-immunity and protection against DENVs, indicating that inoculation with JEV vaccines may influence the distribution of DENVs in co-circulated areas and that the cross-protection induced by JEV vaccines against DENVs might provide important information in terms of DENV prevention.
Eco-friendly streamlined process for sporopollenin exine capsule extraction
Authors: Mundargi Raghavendra C. , Potroz Michael G. , Park Jae Hyeon , Seo Jeongeun , Tan Ee-Lin , Lee Jae Ho , Cho Nam-Joon 
Published: January 2016

AbstractSporopollenin exine capsules (SECs) extracted from Lycopodium clavatum spores are an attractive biomaterial possessing a highly robust structure suitable for microencapsulation strategies. Despite several decades of research into SEC extraction methods, the protocols commonly used for L. clavatum still entail processing with both alkaline and acidolysis steps at temperatures up to 180 °C and lasting up to 7 days. Herein, we demonstrate a significantly streamlined processing regimen, which indicates that much lower temperatures and processing durations can be used without alkaline lysis. By employing CHN elemental analysis, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and dynamic image particle analysis (DIPA), the optimum conditions for L. clavatum SEC processing were determined to include 30 hours acidolysis at 70 °C without alkaline lysis. Extending these findings to proof-of-concept encapsulation studies, we further demonstrate that our SECs are able to achieve a loading of 0.170 ± 0.01 g BSA per 1 g SECs by vacuum-assisted loading. Taken together, our streamlined processing method and corresponding characterization of SECs provides important insights for the development of applications including drug delivery, cosmetics, personal care products and foods.
High efficiency integration of three-dimensional functional microdevices inside a microfluidic chip by using femtosecond laser multifoci parallel microfabrication
Authors: Xu Bing , Du Wen-Qiang , Li Jia-Wen , Hu Yan-Lei , Yang Liang , Zhang Chen-Chu , Li Guo-Qiang , Lao Zhao-Xin , Ni Jin-Cheng , Chu Jia-Ru , Wu Dong , Liu Su-Ling , Sugioka Koji 
Published: January 2016

AbstractHigh efficiency fabrication and integration of three-dimension (3D) functional devices in Lab-on-a-chip systems are crucial for microfluidic applications. Here, a spatial light modulator (SLM)-based multifoci parallel femtosecond laser scanning technology was proposed to integrate microstructures inside a given ‘Y’ shape microchannel. The key novelty of our approach lies on rapidly integrating 3D microdevices inside a microchip for the first time, which significantly reduces the fabrication time. The high quality integration of various 2D-3D microstructures was ensured by quantitatively optimizing the experimental conditions including prebaking time, laser power and developing time. To verify the designable and versatile capability of this method for integrating functional 3D microdevices in microchannel, a series of microfilters with adjustable pore sizes from 12.2 μm to 6.7 μm were fabricated to demonstrate selective filtering of the polystyrene (PS) particles and cancer cells with different sizes. The filter can be cleaned by reversing the flow and reused for many times. This technology will advance the fabrication technique of 3D integrated microfluidic and optofluidic chips.
Air-mode photonic crystal ring resonator on silicon-on-insulator
Authors: Gao Ge , Zhang Yong , Zhang He , Wang Yi , Huang Qingzhong , Xia Jinsong 
Published: January 2016

AbstractIn this report, we propose and demonstrate an air-mode photonic crystal ring resonator (PhCRR) on silicon-on-insulator platform. Air mode is utilized to confine the optical field into photonic crystal (PhC) air holes, which is confirmed by the three-dimensional finite-difference time-domain simulation. PhCRR structure is employed to enhance the light-matter interaction through combining the whispering-gallery mode resonance of ring resonator with the slow-light effect in PhC waveguide. In the simulated and measured transmission spectra of air-mode PhCRR, nonuniform free spectral ranges are observed near the Brillouin zone edge of PhC, indicating the presence of the slow-light effect. A maximum group index of 27.3 and a highest quality factor of 14600 are experimentally obtained near the band edge. Benefiting from the strong optical confinement in the PhC holes and enhanced light-matter interaction in the resonator, the demonstrated air-mode PhCRR is expected to have potential applications in refractive index sensing, on-chip light emitting and nonlinear optics by integration with functional materials.
Bio-inspired Plasmonic Nanoarchitectured Hybrid System Towards Enhanced Far Red-to-Near Infrared Solar Photocatalysis
Authors: Yan Runyu , Chen Min , Zhou Han , Liu Tian , Tang Xingwei , Zhang Ke , Zhu Hanxing , Ye Jinhua , Zhang Di , Fan Tongxiang 
Published: January 2016

AbstractSolar conversion to fuels or to electricity in semiconductors using far red-to-near infrared (NIR) light, which accounts for about 40% of solar energy, is highly significant. One main challenge is the development of novel strategies for activity promotion and new basic mechanisms for NIR response. Mother Nature has evolved to smartly capture far red-to-NIR light via their intelligent systems due to unique micro/nanoarchitectures, thus motivating us for biomimetic design. Here we report the first demonstration of a new strategy, based on adopting nature’s far red-to-NIR responsive architectures for an efficient bio-inspired photocatalytic system. The system is constructed by controlled assembly of light-harvesting plasmonic nanoantennas onto a typical photocatalytic unit with butterfly wings’ 3D micro/nanoarchitectures. Experiments and finite-difference time-domain (FDTD) simulations demonstrate the structural effects on obvious far red-to-NIR photocatalysis enhancement, which originates from (1) Enhancing far red-to-NIR (700~1200 nm) harvesting, up to 25%. (2) Enhancing electric-field amplitude of localized surface plasmon (LSPs) to more than 3.5 times than that of the non-structured one, which promotes the rate of electron-hole pair formation, thus substantially reinforcing photocatalysis. This proof-of-concept study provides a new methodology for NIR photocatalysis and would potentially guide future conceptually new NIR responsive system designs.
Alterations of the cytoskeleton in human cells in space proved by life-cell imaging
Authors: Corydon Thomas J. , Kopp Sascha , Wehland Markus , Braun Markus , Schütte Andreas , Mayer Tobias , Hülsing Thomas , Oltmann Hergen , Schmitz Burkhard , Hemmersbach Ruth , Grimm Daniela 
Published: January 2016

AbstractMicrogravity induces changes in the cytoskeleton. This might have an impact on cells and organs of humans in space. Unfortunately, studies of cytoskeletal changes in microgravity reported so far are obligatorily based on the analysis of fixed cells exposed to microgravity during a parabolic flight campaign (PFC). This study focuses on the development of a compact fluorescence microscope (FLUMIAS) for fast live-cell imaging under real microgravity. It demonstrates the application of the instrument for on-board analysis of cytoskeletal changes in FTC-133 cancer cells expressing the Lifeact-GFP marker protein for the visualization of F-actin during the 24th DLR PFC and TEXUS 52 rocket mission. Although vibration is an inevitable part of parabolic flight maneuvers, we successfully for the first time report life-cell cytoskeleton imaging during microgravity and gene expression analysis after the 31st parabola showing a clear up-regulation of cytoskeletal genes. Notably, during the rocket flight the FLUMIAS microscope reveals significant alterations of the cytoskeleton related to microgravity. Our findings clearly demonstrate the applicability of the FLUMIAS microscope for life-cell imaging during microgravity, rendering it an important technological advance in live-cell imaging when dissecting protein localization.
Multiphoton photochemical crosslinking-based fabrication of protein micropatterns with controllable mechanical properties for single cell traction force measurements
Authors: Tong Ming Hui , Huang Nan , Zhang Wei , Zhou Zhuo Long , Ngan Alfonso Hing Wan , Du Yanan , Chan Barbara Pui 
Published: January 2016

AbstractEngineering 3D microstructures with predetermined properties is critical for stem cell niche studies. We have developed a multiphoton femtosecond laser-based 3D printing platform, which generates complex protein microstructures in minutes. Here, we used the platform to test a series of fabrication and reagent parameters in precisely controlling the mechanical properties of protein micropillars. Atomic force microscopy was utilized to measure the reduced elastic modulus of the micropillars and transmission electron microscopy was used to visualize the porosity of the structures. The reduced elastic modulus of the micropillars associated positively and linearly with the scanning power. On the other hand, the porosity and pore size of the micropillars associated inversely and linearly with the scanning power and reagent concentrations. While keeping the elastic modulus constant, the stiffness of the micropillars was controlled by varying their height. Subsequently, the single cell traction forces of rabbit chondrocytes, human dermal fibroblasts, human mesenchymal stem cells and bovine nucleus pulposus cells (bNPCs) were successfully measured by culturing the cells on micropillar arrays of different stiffness. Our results showed that the traction forces of all groups showed positive relationship with stiffness and that the chondrocytes and bNPCs generated the highest and lowest traction forces, respectively.
Interictal spikes during sleep are an early defect in the Tg2576 mouse model of β-amyloid neuropathology
Authors: Kam Korey , Duffy Áine M. , Moretto Jillian , LaFrancois John J. , Scharfman Helen E. 
Published: January 2016

AbstractIt has been suggested that neuronal hyperexcitability contributes to Alzheimer’s disease (AD), so we asked how hyperexcitability develops in a common mouse model of β-amyloid neuropathology - Tg2576 mice. Using video-EEG recordings, we found synchronized, large amplitude potentials resembling interictal spikes (IIS) in epilepsy at just 5 weeks of age, long before memory impairments or β-amyloid deposition. Seizures were not detected, but they did occur later in life, suggesting that IIS are possibly the earliest stage of hyperexcitability. Interestingly, IIS primarily occurred during rapid-eye movement (REM) sleep, which is notable because REM is associated with increased cholinergic tone and cholinergic impairments are implicated in AD. Although previous studies suggest that cholinergic antagonists would worsen pathophysiology, the muscarinic antagonist atropine reduced IIS frequency. In addition, we found IIS occurred in APP51 mice which overexpress wild type (WT)-APP, although not as uniformly or as early in life as Tg2576 mice. Taken together with results from prior studies, the data suggest that surprising and multiple mechanisms contribute to hyperexcitability. The data also suggest that IIS may be a biomarker for early detection of AD.
Force-dependent switch in protein unfolding pathways and transition-state movements
Authors: Zhuravlev Pavel I. , Hinczewski Michael , Chakrabarti Shaon , Marqusee Susan , Thirumalai D. 
Published: January 2016

Significance
Single-domain proteins with symmetrical arrangement of secondary structural elements in the native state are expected to fold by diverse pathways. However, understanding the origins of pathway diversity, and the experimental signatures for identifying it, are major challenges, especially for small proteins with no obvious symmetry in the folded states. We show rigorously that upward curvature in the logarithm of unfolding rates as a function of force (or denaturants) implies that the folding occurs by diverse pathways. The theoretical concepts are illustrated using simulations of src SH3 domain, which explain the emergence of parallel pathways in single-molecule pulling experiments and provide structural description of the routes to the unfolded state. We make testable predictions illustrating the generality of the theory.
Quantifying the metallurgical response of a nuclear steel to welding thermal cycles
Authors: O'Meara N. , Abdolvand H. , Francis J. A. , Smith S. D. , Withers P. J. 
Published: January 2016

Solid-state phase transformations can drastically influence the evolution of stress in welds due to the strains associated with the transformations and related changes in mechanical properties. As such, finite-element predictions of welding residual stresses need reliable materials data including, where applicable, information on phase transformation kinetics and phase- and temperature-dependent material properties. Owing to a scarcity of such data, many authors have used uncalibrated empirical modelling approaches for the prediction of welding residual stresses. This paper addresses this critical shortage for an important nuclear pressure vessel (SA508) steel. Austenite formation, grain growth and decomposition data are presented and subsequently used to calibrate transformation models. These models are shown to accurately predict microstructure and residual stresses for experimental test cases. This paper is part of a Themed Issue on Measurement, modelling and mitigation of residual stress.
Age and Growth of Atlantic Sturgeon in the New York Bight
Authors: Dunton Keith J. , Jordaan Adrian , Secor David H. , Martinez Christopher M. , Kehler Thomas , Hattala Kathy A. , Van Eenennaam Joel P. , T. Fisher Matthew , McKown Kim A. , Conover David O. , Frisk Michael G. 
Published: January 2016

Abstract
Accurate estimates of age and growth of fishes are important in the management and conservation of species and for the development of modeling approaches. Assessments of endangered or rare species typically are limited by poor or inadequate data owing to low abundance, unrepresentative sampling, and/or restrictions on sampling. Atlantic Sturgeon Acipenser oxyrinchus oxyrinchus, which occurs along the east coast of North America, has five distinct population segments (DPSs) listed under the U.S. Endangered Species Act. The New York Bight (NYB) DPS is listed as endangered and represents the largest DPS in the United States. Coastal trawl surveys from 2005 to 2012 were used to evaluate the current age structure of the NYB DPS. A total of 21 year-classes (mean age = 8.89 years, n = 742 fish) were observed. Age data for the NYB DPS were combined with other available age estimates from multiple research laboratories and sources (n = 2,774) in the Hudson River and Delaware River as well from the coastal regions of New York, New Jersey, and Delaware from 1975 to 2012. Collectively, the combined data set captured much of the age range of the species, minimizing age biases and resulting in improved von Bertalanffy parameter estimates (L∞ = 278.87, K = 0.057, t0 = −1.27) with high overall model fit (r2 = 0.87). We assessed the effects of individual data sets through a series of leave-one-out bootstrap routines that evaluated the influence of each data set on growth parameter estimates. The parameter estimates of the von Bertalanffy growth function were influenced by sampling location and/or researcher effects. Despite these differences, the combined data set approach used here represents the most comprehensive study on the age-and-growth relationship of Atlantic Sturgeon and provides parameter estimates for the development of population dynamics models and valuable information for future management.
Received February 23, 2015; accepted September 24, 2015
Predation on Chinook Salmon Parr by Hatchery Salmonids and Fallfish in the Salmon River, New York
Authors: Johnson James H. , Nack Christopher C. , Chalupnicki Marc A. , Abbett Ross , McKenna J. E. 
Published: January 2016

Abstract
Naturally reproduced Chinook Salmon Oncorhynchus tshawytscha contribute substantially to the fishery in Lake Ontario. The Salmon River, a Lake Ontario tributary in New York, produces the largest numbers of naturally spawned Chinook Salmon, with parr abundance in the river often exceeding 10 million. In the spring of each year, large numbers of hatchery salmonid yearlings—potential predators of Chinook Salmon parr—are released into the Salmon River by the New York State Department of Environmental Conservation. We sought to examine predation on Chinook Salmon parr in the Salmon River during May and June prior to out-migration. Over the 4 years examined (2009–2012), annual consumption of Chinook Salmon parr by hatchery-released yearling steelhead O. mykiss and Coho Salmon O. kisutch ranged from 1.5 to 3.3 million and from 0.4 to 2.1 million, respectively. In 2009, Fallfish Semotilus corporalis were estimated to consume 2.9 million Chinook Salmon parr. Predation was higher in May, when the average TL of Chinook Salmon parr was 44.5 mm, than in June. Fallfish were also important predators of naturally reproduced steelhead subyearlings, consuming an estimated 800,000 steelhead in 2009. Hatchery-released yearling salmonids consumed 13.8–15.3% of the Chinook Salmon parr that were estimated to be present in the Salmon River during 2010–2012. Earlier releases of hatchery salmonid yearlings could reduce the riverine consumption of Chinook Salmon parr by facilitating the out-migration of yearlings prior to Chinook Salmon emergence.
Received February 23, 2015; September 29, 2015