bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of SSF on Diagnostic Performance of Coronary Computed Tomography Angiography Within 1 Heart Beat in Patients With High Heart Rate Using a 256-Row Detector Computed Tomography
Authors: Liang Junfu , Wang Hui , Xu Lei , Dong Li , Fan Zhanming , Wang Rui , Sun Zhonghua 
Published: July 2017

Objective
The aim of this study was to investigate the impact of a motion-correction algorithm on diagnostic accuracy of coronary computed tomography angiography (CCTA) within 1 heart beat in patients with high heart rate (HR) using a 256-row detector CT.

Method
Sixty-four consecutive patients with known or suspected coronary artery disease (symptomatic) and with HR of 75 beats per minute or greater (mean [SD] HR, 82.6 [7.3] beats per minute) undergoing CCTA and invasive coronary angiography within 4 weeks were prospectively enrolled. Coronary computed tomography angiography was performed with a 256-row detector CT (Revolution CT, GE Healthcare) using prospectively electrocardiography-triggered volume scan in 1 heart beat. All images were reconstructed using standard (STD) algorithm and a motion-correction algorithm reconstruction (Snapshot Freeze SSF; GE Healthcare) technique. The image quality of coronary artery segments was evaluated by 2 experienced radiologists using a 4-point scale based on the 18-segment model. Diagnostic accuracy was compared between STD and SSF for significant lumen stenosis (≥50%) of each segment with invasive coronary angiography as the reference standard for determining significant stenosis.

Results
The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value with STD and SSF were 93.7%, 85.1%, 50.2%, and 98.8% versus 91.9%, 95.8%, 77.9%, and 98.7% on per-segment assessment; 98.7%, 74.0%, 62.9%, and 99.2% versus 96.2%, 94.4%, 77.9%, and 98.7% on per-artery assessment; and 100%, 14.3%, 70.5%, and 100% versus 100%, 85.7%, 93.5%, and 100% on per-patient assessment, respectively. There was a significant difference in accuracy between STD and SFF on per-patient level 71.9% versus 95.3%, on per-artery level 81.6% versus 94.9%, and per-segment level 86.3% versus 95.3%, respectively. The area under receiver operating characteristics curve analysis also showed a significant improvement on diagnostic performance with the SSF technique versus with the STD algorithm on per-patient level (P < 0.001), with corresponding area under the curve being 0.91 (95% confidence interval, 0.79–1.00) and 0.60 (95% confidence interval, 0.44–0.75). The mean effective dose was 2.0 mSv.

Conclusions
Coronary computed tomography angiography can be performed in patients with high HR within 1 heart beat yielding low radiation dose. The use of SSF technique reconstruction for 1 heart beat CCTA achieves significant improvements in image quality and diagnostic value.
Predicting Neoadjuvant Chemotherapy in Nonconcentric Shrinkage Pattern of Breast Cancer Using 1H-Magnetic Resonance Spectroscopic Imaging
Authors: Zhou Juan , Qiao Peng-Gang , Zhang Hong-Tao , Li Gong-Jie , Jiang Ze-Fei 
Published: July 2017

ObjectiveThe aim of this study was to explore the response to neoadjuvant chemotherapy (NAC) in nonconcentric shrinkage pattern of breast cancer (BC) patients using1H–magnetic resonance spectroscopy.MethodsTwenty-five BC patients were the study cohort. All patients received AT-based regimen as first-line treatment. Tumor response to chemotherapy was evaluated after the second and fourth cycles using magnetic resonance imaging and magnetic resonance spectroscopy. Final histopathology following surgery after 4 to 8 cycles of NAC served as a reference. Changes in total choline integral* (tCho) and tumor size in response versus nonresponse groups were compared using the 2-way Mann-Whitney nonparametric test. Receiver operating characteristic (ROC) analyses were undertaken, and the area under the ROC curve compared among them.Results1H–magnetic resonance spectroscopy revealed a negative tCho integral* in 6 cases at the first follow-up and 14 cases at the second follow-up. Based on pathology (Miller-Payne system), there were 16 cases of response, and 9 cases of nonresponse. The tCho integral* was significantly different between the response and nonresponse groups at the second follow-up (P= 0.027). The tumor size changes were not significantly different in the response and nonresponse groups at the second follow-up study (P> 0.05). The comparison of ROC curves among the change in tCho integral* and tumor size at baseline and both follow-ups revealed the maximum area under the ROC curve of the change in tCho integral* to be 0.747 at the second follow-up, sensitivity to be 93.75%, and positive predictive value to be 78.9%.ConclusionsIn nonconcentric shrinkage pattern after NAC of BC, when tumor size is difficult to reflect the response, tCho integral* reduction may be a predictive marker.
The rumen microbiome: an underexplored resource for novel antimicrobial discovery
Authors: Oyama Linda B , Girdwood Susan E , Cookson Alan R , Fernandez-Fuentes Narcis , Privé Florence , Vallin Hannah E , Wilkinson Toby J , Golyshin Peter N , Golyshina Olga V , Mikut Ralf , Hilpert Kai , Richards Jennifer , Wootton Mandy , Edwards Joan E , Maresca Marc , Perrier Josette , Lundy Fionnuala T , Luo Yu , Zhou Mei , Hess Matthias , Mantovani Hilario C , Creevey Christopher J , Huws Sharon A 
Published: July 2017

AbstractAntimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, 2 and 3) were effective against numerous bacterial pathogens, including methicillin resistantStaphylococcus aureus(MRSA). No decrease in MRSA susceptibility was observed after 25 days of sub-lethal exposure to these AMPs. The AMPs bound preferentially to bacterial membrane lipids and induced membrane permeability leading to cytoplasmic leakage. Topical administration of Lynronne-1 (10% w/v) to a mouse model of MRSA wound infection elicited a significant reduction in bacterial counts, which was comparable to treatment with 2% mupirocin ointment. Our findings indicate that the rumen microbiome may provide viable alternative antimicrobials for future therapeutic application.
CRISPR/Cas9-mediated Knock-in of an Optimized TetO Repeat for Live Cell Imaging of Endogenous Loci
Authors: Tasan Ipek , Sustackova Gabriela , Zhang Liguo , Kim Jiah , Sivaguru Mayandi , HamediRad Mohammad , Wang Yuchuan , Genova Justin , Ma Jian , Belmont Andrew S. , Zhao Huimin 
Published: July 2017

AbstractNuclear organization has an important role in determining genome function; however, it is not clear how spatiotemporal organization of the genome relates to functionality. To elucidate this relationship, a high-throughput method for tracking any locus of interest is desirable. Here, we report an efficient and scalable method named SHACKTeR (Short Homology and CRISPR/Cas9-mediated Knock-in of a TetO Repeat) for live cell imaging of specific chromosomal regions. Compared to alternatives, our method does not require a nearby repetitive sequence and it requires only two modifications to the genome: CRISPR/Cas9-mediated knock-in of an optimized TetO repeat and its visualization by TetR-EGFP expression. Our simplified knock-in protocol, utilizing short homology arms integrated by PCR, was successful at labeling 9 different loci in HCT116 cells with up to 20% efficiency. These loci included both nuclear speckle-associated, euchromatin regions and nuclear lamina-associated, heterochromatin regions. We anticipate the general applicability and scalability of our method will enhance causative analyses between gene function and compartmentalization in a high-throughput manner.
Integration of learning organization ideas and Islamic core values principle at university
Authors: Ahmad Aini , Sulan Norrihan , Abdul Rani Anita 
Published: July 2017

Purpose
The concept of learning organization has been inspiring both public and private sectors since the early 90s. This paper discusses the implementation of learning organization ideas along with the organization’s core values at one of the public universities in Malaysia. The purpose of this paper is to identify the components of core values that support the development and maintenance of learning organization ideas. It also aims to examine the potential integration of the learning organization idea with organizational core values.

Design/methodology/approach
The study analyses the application of learning organization principles based on Örtenblad’s (2002) four understandings of learning organization, which are organizational learning, learning at work, learning climate and learning structure, along with the five core values initiatives at the university.

Findings
This study suggests that the university core values and the learning organization idea work harmoniously with each other. This paper provides examples of the application of the learning organization idea and the core values attribute and how both fit into each other in one of the Malaysian public universities. A learning organization–core values matrix is proposed at the end of the paper.

Research limitations/implications
This research uses the specific case of organizational core values that are established based on Islamic principles. Opportunities for further research can be a study of learning organizations in other core values environment and also from a different belief system.

Practical implications
Learning organization is relevant and in harmony with Islamic environment.

Originality/value
The paper provides real examples of learning organization practices within the core values agenda in a Malaysian university.
Bibliotherapeutic book club intervention to promote reading skills and social–emotional competencies in low SES community‐based high schools: A randomised controlled trial
Authors: Tijms Jurgen , Stoop Mirthe A. , Polleck Jody N. 
Published: July 2017

Reading skills and social–emotional competencies are two important skills for both academic achievement and long‐term quality of life. The present study evaluated the effects of a school‐based intervention to promote reading skills and social–emotional competencies in young adolescents from urban, low socioeconomic status (SES) communities in their first year of secondary education. A small group intervention was conducted, using book clubs in which students read and discussed literature with the objective to enhance reading attitude, reading comprehension and social–emotional competencies. Participants were randomly allocated to either the book club intervention condition (n = 50) or a business‐as‐usual control condition (n = 40). Results revealed that the intervention group accrued significant greater gains than the control group in (recreational) reading attitude, reading comprehension and social emotional competences.HighlightsWhat is already known about this topic

The transition from primary to secondary education is accompanied by a sharp decline in reading attitude and a rise in demands on young adolescents' social–emotional competencies.
Qualitative research suggests book clubs can be an educational tool to enhance both reading behaviour and social–emotional skills.
What this paper adds

A bibliotherapeutic book club intervention can be an effective tool to improve reading attitudes, reading comprehension and to strengthen social–emotional competencies in young adolescents from low SES areas.
Implications for theory, policy or practice

This study supports the inclusion of bibliotherapeutic book clubs in the secondary education curriculum as a mean to address both reading behaviour and social–emotional competencies, two skills that are important for adolescents' academic success.
Perceptions of HR practices, person–organisation fit, and affective commitment: The moderating role of career stage
Authors: Kooij Dorien T.A.M. , Boon Corine 
Published: July 2017

AbstractIn a three‐wave survey study among 487 Dutch university employees, we examined how and when employees' perceptions of high‐performance work practices (HPWP) affect employee affective commitment. We proposed that perceived person–organisation (PO) fit mediates this relationship and that the relationships among perceptions of HPWP, perceived PO fit, and affective commitment differ across career stages. Our results confirm that perceptions of HPWP enhance PO fit perceptions and, in turn, strengthen affective commitment. Moreover, the relationship between perceptions of HPWP and perceived PO fit is only significant among employees in the advancement stage of their careers; however, direct comparison across different career stages reveals no significant differences in the HPWP–PO fit relationship. Furthermore, career stage partly moderates the relationship between PO fit and affective commitment, which is only significant among employees in the maintenance career stage. These findings have important implications for the literature on strategic Human Resource Management and PO fit.
Information disclosure quality: correlation versus precision
Authors: Cheung Adrian (Wai‐Kong) , Hu Wei 
Published: July 2017

AbstractWe investigate how a multidimensional disclosure quality (i.e., correlation and precision) determines an optimal information disclosure strategy. We find that, for an infinitely lived, unlevered firm with market perfection, a truth‐telling disclosure is optimal at increasing the expected firm value. However, for a finitely lived, levered firm in the presence of market imperfections (e.g., bankruptcy cost), the optimal disclosure quality depends negatively on the level of imperfections. Once we consider the agency problem, such dependence can become positive, thereby highlighting the importance of a proper managerial‐incentive scheme to align the information disclosure interests of managers and shareholders.
The OregonHeart Total Artificial Heart: Design and Performance on a Mock Circulatory Loop
Authors: Glynn Jeremy , Song Howard , Hull Bryan , Withers Stanley , Gelow Jill , Mudd James , Starr Albert , Wampler Richard 
Published: July 2017

AbstractWidespread use of heart transplantation is limited by the scarcity of donor organs. Total artificial heart (TAH) development has been pursued to address this shortage, especially to treat patients who require biventricular support. We have developed a novel TAH that utilizes a continuously spinning rotor that shuttles between two positions to provide pulsatile, alternating blood flow to the systemic and pulmonary circulations without artificial valves. Flow rates and pressures generated by the TAH are controlled by adjusting rotor speed, cycle frequency, and the proportion of each cycle spent pumping to either circulation. To validate the design, a TAH prototype was placed in a mock circulatory loop that simulates vascular resistance, pressure, and compliance in normal and pathophysiologic conditions. At a systemic blood pressure of 120/80 mm Hg, nominal TAH output was 7.4 L/min with instantaneous flows reaching 17 L/min. Pulmonary artery, and left and right atrial pressures were all maintained within normal ranges. To simulate implant into a patient with severe pulmonary hypertension, the pulmonary vascular resistance of the mock loop was increased to 7.5 Wood units. By increasing pump speed to the pulmonary circulation, cardiac output could be maintained at 7.4 L/min as mean pulmonary artery pressure increased to 56 mm Hg while systemic blood pressures remained normal. This in vitro testing of a novel, shuttling TAH demonstrated that cardiac output could be maintained across a range of pathophysiologic conditions including pulmonary hypertension. These experiments serve as a proof‐of‐concept for the design, which has proceeded to in vivo testing.
Laparoendoscopic single‐site surgery in inguinal hernia repair
Authors: Buenafe Alfred Allen E. , Lee‐Ong Alembert C. 
Published: July 2017

AbstractLaparoendoscopic single‐site surgery (LESS) for laparoscopic inguinal hernia repair (IHR) offers the potential for excellent cosmetic outcomes and maximization of the inherent advantages of minimally invasive surgery. LESS IHR is associated with a steep learning curve, which is attributable to both the IHR technique itself and the single‐site technique. The technical obstacles in the single‐site technique may be mitigated by employing certain maneuvers and strategies that minimize clashing between instruments and improve freedom of movement. The current literature consistently points out the inherent challenges in LESS IHR, with its longer operative time compared to that of conventional laparoscopic IHR. LESS IHR performed by capable operators has comparable complication rates, duration of hospital stay, and incidence of recurrence as conventional laparoscopic IHR. LESS IHR is both feasible and safe. Given its excellent cosmetic outcome, it is likely to be sought by younger patients who are concerned with scar formation. The use of robotics may bypass the technical challenges in LESS, but cost considerations in their usage will likely persist.
Identification of unique proteomic signatures in allergic and non‐allergic skin disease
Authors: Wang J. , Suárez‐Fariñas M. , Estrada Y. , Parker M. L. , Greenlees L. , Stephens G. , Krueger J. , Guttman‐Yassky E. , Howell M. D. 
Published: July 2017

SummaryBackgroundAtopic dermatitis (AD), psoriasis (PS), and contact dermatitis (CD) are common skin diseases, characterized by barrier disruption and systemic inflammation, with unique epidermal signatures and common inflammatory pathways identified by transcriptomic profiling. This study profiled proteomic signatures in serum from subjects with AD, PS, and CD compared with healthy controls (HC).ObjectiveIdentify unique proteomic signatures to distinguish between inflammatory diseases with similar epidermal disruption and overlapping epithelial inflammation.MethodsSera from 20 subjects with moderate to severe AD, 10 subjects with CD, 12 subjects with moderate to severe PS, 10 subjects with both AD and CD, and 10 HC with no history of skin disease was analysed using high‐throughput proteomic analysis that detects expression of 1129 protein targets. Protein expression was compared between disease and HC, and across diseases for statistical significance (fold change≥1.5 and false discovery rate≤0.05), to identify unique proteomic signatures for each disease.ResultsComplement C5A anaphylatoxin (C5A), lipopolysaccharide binding protein (LBP), C‐reactive protein (CRP), ILT‐4, C‐C motif ligand 18 (PARC), and sialic acid‐binding Ig‐like lectin 14 (SIG14) were significantly modulated in all three diseases compared with HC. We identified unique signatures for AD (Immunoglobulin E (IgE), thymus‐ and activation‐regulated chemokine (TARC) and macrophage‐derived chemokine (MDC)), CD (10 proteins), and PS (kynureninase (KYNU)). Proteomic profiling in subjects with both AD and CD identified additional dysregulated proteins compared with subjects with either condition alone, indicating an exacerbated inflammation reaction.Conclusions and Clinical RelevanceUnique sera proteomic signatures may distinguish between inflammatory skin diseases despite similar epidermal barrier disruption and epithelial inflammation. This may provide insight into disease pathogenesis, diagnosis, and therapeutic intervention in difficult‐to‐treat subjects.
Tissue dynamics and regenerative outcome in two resorbable non‐cross‐linked collagen membranes for guided bone regeneration: A preclinical molecular and histological study in vivo
Authors: Omar Omar , Dahlin Anna , Gasser Angelines , Dahlin Christer 
Published: July 2017

AbstractObjectivesTo investigate the molecular and structural patterns of bone healing during guided bone regeneration (GBR), comparing two resorbable non‐cross‐linked collagen membranes.Materials and methodsTrabecular bone defects in rat femurs were filled with deproteinized bovine bone (DBB) and covered with either a membrane comprising collagen and elastin (CXP) or collagen (BG). Samples were harvested after 3 and 21 days for histology/histomorphometry and gene expression analysis. Gene expression analysis was performed on the membrane (at 3 days) and the underlying defect compartment (at 3 and 21 days).ResultsAt the total defect level, no differences in bone area percentage were found between the CXP and BG. When evaluating the central area of the defect, a higher percentage of de novo bone formation was seen for the CXP membrane (34.9%) compared to BG (15.5%) at 21 days (p = .01). Gene expression analysis revealed higher expression of bone morphogenetic protein‐2 (Bmp2) in the membrane compartment at 3 days in the BG group. By contrast, higher Bmp2 expression was found in the defect compartment treated with the CXP membrane, both at 3 and 21 days. A significant temporal increase (from 3 to 21 days) in the remodeling activity, cathepsin K (Catk) and calcitonin receptor (Calcr), was found in the CXP group. Molecular analysis demonstrated expression of several growth factors and cytokines in the membrane compartment irrespective of the membrane type. Bmp2 expression in the membrane correlated positively with Bmp2 expression in the defect, whereas fibroblast growth factor‐2 (Fgf2) expression in the membrane correlated positively with inflammatory cytokines, tumor necrosis factor‐alpha (Tnfa) and interleukin‐6 (Il6) in the defect.ConclusionsThe results provide histological and molecular evidence that different resorbable collagen membranes contribute differently to the GBR healing process. In the BG group, bone formation was primarily localized to the peripheral part of the defect. By contrast, the CXP group demonstrated significantly higher de novo bone formation in the central portion of the defect. This increase in bone formation was reflected by triggered expression of potent osteogenic growth factor, Bmp2, in the defect. These findings suggest that the CXP membrane may have a more active role in regulating the bone healing dynamics.
Effectiveness of protected areas for vertebrates based on taxonomic and phylogenetic diversity
Authors: Quan Qing , Che Xianli , Wu Yongjie , Wu Yuchun , Zhang Qiang , Zhang Min , Zou Fasheng 
Published: July 2017

AbstractEstablishing protected areas is the primary goal and tool for preventing irreversible biodiversity loss. However, the effectiveness of protected areas that target specific species has been questioned for some time because targeting key species for conservation may impair the integral regional pool of species diversity and phylogenetic and functional diversity are seldom considered. We assessed the efficacy of protected areas in China for the conservation of phylogenetic diversity based on the ranges and phylogenies of 2279 terrestrial vertebrates. Phylogenetic and taxonomic diversity were strongly and positively correlated, and only 12.1–43.8% of priority conservation areas are currently protected. However, the patterns and coverage of phylogenetic diversity were affected when weighted by species richness. These results indicated that in China, protected areas targeting high species richness protected phylogenetic diversity well overall but failed to do so in some regions with more unique or threatened communities (e.g., coastal areas of eastern China, where severely threatened avian communities were less protected). Our results suggest that the current distribution of protected areas could be improved, although most protected areas protect both taxonomic and phylogenetic diversity.