bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arabidopsis B‐cell lymphoma2 (Bcl‐2)‐associated athanogene 7 (BAG7)‐mediated heat tolerance requires translocation, sumoylation and binding to WRKY29
Authors: Li Yurong , Williams Brett , Dickman Martin 
Published: December 2016

Summary

To cope with stress and increased accumulation of misfolded proteins, plants and animals use a survival pathway known as the unfolded protein response (UPR) that signals between the endoplasmic reticulum (ER) and the nucleus to maintain cell homeostasis via proper folding of proteins. B‐cell lymphoma2 (Bcl‐2)‐associated athanogene (BAG) proteins are an evolutionarily conserved family of co‐chaperones that are linked to disease states in mammals and responses to environmental stimuli (biotic and abiotic) in plants.

Molecular and physiological techniques were used to functionally characterize a newly identified branch of the UPR initiated by the ER‐localized co‐chaperone from Arabidopsis thaliana, AtBAG7.

AtBAG7 has functional roles in both the ER and the nucleus. Upon heat stress, AtBAG7 is sumoylated, proteolytically processed and translocated from the ER to the nucleus, where interaction with the WRKY29 transcription factor occurs. Sumoylation and translocation are required for the AtBAG7–WRKY29 interaction and subsequent stress tolerance. In the ER, AtBAG7 interacts with the ER‐localized transcription factor, AtbZIP28, and established UPR regulator, the AtBiP2 chaperone.

The results indicate that AtBAG7 plays a central regulatory role in the heat‐induced UPR pathway.
Nitrogen Limitation Adaptation (NLA) is involved in source‐to‐sink remobilization of nitrate by mediating the degradation of NRT1.7 in Arabidopsis
Authors: Liu Wenwen , Sun Qing , Wang Kai , Du Qingguo , Li Wen‐Xue 
Published: December 2016

Summary

Recent studies on nitrate transporters (NRTs) have greatly increased our knowledge of the mechanisms regulating nitrogen (N) homeostasis in plants. However, an understanding of how these NRTs are regulated is still lacking.

The nitrogen limitation adaptation (nla) mutant is hypersensitive to N limitation. In the nla mutant, 15N‐nitrate spotted on old leaves preferentially accumulated in the youngest leaves. Analysis of leaf cross‐sections indicated that NLA expression was expressed in the sieve element and companion cell system. The results of bimolecular fluorescence complementation (BiFC), split‐ubiquitin yeast two‐hybrid and co‐immunoprecipitation (CoIP) assays demonstrated that NLA interacts with NRT1.7 in the plasma membrane.

The following findings suggest that NLA directs the ubiquitination of NRT1.7: the down‐regulation of NRT1.7 protein abundance in 35S::NLA/35S::Myc‐NRT1.7 double transgenic plants compared with 35S::Myc‐NRT1.7 transgenic plants; the up‐regulation of NRT1.7 protein abundance in the nla mutant compared with wild‐type plants; and the direct degradation of truncated NRT1.7 recombinant protein by NLA. Furthermore, analysis of NLA and NRT1.7 protein abundance in mirna827 knock‐out plants showed that N deficiency‐guided translational repression of NLA depends on miRNA827.

Our findings reveal that plants regulate source‐to‐sink remobilization of nitrate by the ubiquitin‐mediated post‐translational regulatory pathway.
Accommodation stimulus and response determinations with autorefractors
Authors: Atchison David A. , Varnas Saulius R. 
Published: December 2016

AbstractPurposeTo develop equations for accommodation stimulus and accommodation response with autorefractors when the accommodation stimulus is produced by combinations of object distances and lenses placed in front of eyes, and to give worked examples using these equations.MethodsSimple ray tracing was used to determine stimulus and response equations, taking into account the reference positions for targets, for refraction, and for autorefractor readings.ResultsSeveral examples applying equations are provided. Features of these examples include evaluating approximate calculations that have been used previously, demonstrating which equations should be used in different circumstances, how to substitute numbers into equations, how to deal with discrepancies between subjective and objective refraction, and how to deal with astigmatism. Problems associated with measuring accommodation response by placing lenses in front of the eye are discussed.ConclusionsAccurate equations for accommodation stimulus and accommodation response for a range of accommodation stimuli in different setups have been developed.
Anterior and Posterior Instrumentation with Different Debridement and Grafting Procedures for Multi‐Level Contiguous Thoracic Spinal Tuberculosis
Authors: Cui Xu , Li Li‐tao , Ma Yuan‐zheng 
Published: December 2016

ObjectiveTo evaluate the clinical outcomes of anterior and posterior instrumentation with different debridement and graft fusion methods for multi‐level contiguous thoracic spinal tuberculosis.MethodsWe retrospectively evaluated 81 patients with multi‐level contiguous thoracic spinal tuberculosis who underwent anterior or posterior instrumentation combined with different methods of debridement, decompression, and graft fusion from January 2002 to December 2012. All patients were divided into an anterior instrumentation group and a posterior instrumentation group. In the anterior instrumentation group, there were 39 patients who underwent transthoracic debridement. In the posterior instrumentation group, there were 34 patients who underwent trans‐costotransverse decompression and strut grafting with posterior instrumentation, and another 8 patients underwent combined anterior debridement and strut grafting with posterior instrumentation in a single‐stage or two‐stage procedure. The kyphotic angles were calculated from lateral spinal X‐rays using the modified Konstam method. The symptoms and signs of tuberculosis, fusion level, fusion time of the bone graft, average kyphosis angle, average correction, average loss of correction, and clinical complications were recorded. The average follow‐up period was 37 months (range, 17–72 months).ResultsThe cohort consisted of 47 males and 34 females with an average age of 38 years. The mean durations of the operations were 3.5 ± 0.4 h in the anterior group and 4.0 ± 0.3 h in the posterior group (
P
< 0.05). The mean blood loss volumes during surgery were 450 ± 42 and 560 ± 51 mL for the anterior group and the posterior group, respectively (
P
< 0.01). The kyphotic deformities were corrected from 32.1° ± 10.3° to 10.2° ± 2.1° in the anterior group and from 33.8° ± 11.7° to 12.6° ± 2.7° in the posterior group (
P
< 0.01). The neurologic statuses of the 23 patients with preoperative neurologic deficits improved in each group. Fusion was confirmed radiographically at 5.4 ± 1.2 months (range, 4–12 months) in the anterior group and 5.6 ± 1.4 months (range, 4–13 months) in the posterior group (
P
> 0.05). Postoperative relapses were noted in 1 and 3 patients in the anterior and the posterior group, respectively.ConclusionPosterior instrumentation was more effective than anterior instrumentation in the correction of kyphosis and the maintenance of the correction. However, postoperative sinus formation was more frequent in patients who underwent a single‐stage posterior procedure.
Swimming attendance during childhood and development of asthma: Meta‐analysis
Authors: Valeriani Federica , Protano Carmela , Vitali Matteo , Romano Spica Vincenzo 
Published: December 2016

AbstractBackgroundThe association between asthma and swimming pool attendance has not been demonstrated and currently there are conflicting results. In order to clarify the association between asthma diagnosis in children and swimming pool attendance, and to assess the consistency of the available epidemiological studies, we completed a literature analysis on the relationship between the exposure to disinfection by‐products in indoor swimming pools during childhood and asthma diagnosis.MethodsFollowing the Meta‐analysis of Observational Studies in Epidemiology (MOOSE) and Preferred Reporting Items for Systematic reviews and Meta‐Analyses (PRISMA) criteria, a systematic review and meta‐analysis was performed by searching MEDLINE via PubMed, TOXNET, and Scopus databases (from inception to 20 April 2015) using the key word “Asthma” together with “swimming pool”, “disinfection by‐products”, “indoor air pollution” and “children”. Inclusion criteria were: English language, a complete analytic study design involving a cohort of children (0–16 years), a well‐defined definition of exposure, and the presence of data on effect and variance. Studies on in vivo, in vitro or professional and accidental exposure were excluded.ResultsAfter a screening process, seven reports (n = 5851 subjects) were included out of a total of 2928 references. The reported OR of the association between swimming pool attendance and asthma prevalence ranged from 0.58 to 2.30. The present meta‐analysis failed to identify a significant difference in asthma development between children attending swimming pools and controls (OR, 1.084; 95% CI: 0.89–1.31).ConclusionsSwimming in childhood does not increase the likelihood of doctor‐diagnosed asthma. Based on this meta‐analysis review, the association of the disease with indoor pool attendance is still unclear.
40Ar/39Ar Geochronology of Volcanic and Intrusive Rocks in the Papandayan Metallic Prospect Area, West Java, Indonesia
Authors: Titisari Anastasia Dewi , Phillips David , Prayatna  , Setyaraharja Eko Purwanto 
Published: December 2016

AbstractThis study presents new 40Ar/39Ar ages on the volcanic and intrusive rocks from the Papandayan metallic district in West Java, Indonesia. The vein system in the Arinem area, one of the prospective areas in the district, has been considered as an epithermal gold–silver–base metal deposit, however, no published age results are available for the host volcanic rocks in the district. The ages of these rocks are critical in terms of their association with mineralization and are important to understand the evolution of volcanism in the region, which has implications for mineral exploration in the district. 40Ar/39Ar plateau ages of two typical basalt and one andesite samples of the Jampang Formation volcanic rocks yielded ages of 11.65 ± 0.52 Ma, 18.15 ± 0.46 Ma and 7.69 ± 0.05 Ma, respectively. 40Ar/39Ar ages of three intrusive rock samples from Gunung Halang diorite, Gunung Lingga diorite, and Gunung Buligir fine‐grained quartz diorite yielded ages of 12.98 ± 0.20 Ma, 10.81 ± 0.15 Ma, and 7.37 ± 0.05 Ma, respectively. The age of the youngest fine‐grained diorite (Gunung Wayang dike) is 3.95 ± 0.03 Ma. An 40Ar/39Ar age obtained from adularia in the Arinem mineralized vein (18.30 ± 0.20 Ma) is older than the age of altered basalt sample of this study (11.65 ± 0.52 Ma) and the K–Ar illite ages of the Arinem vein (9.4 ± 0.3 Ma and 8.8 ± 0.3 Ma) which resulted from a previous study. The age results suggest that the Papandayan district may have experienced multiple hydrothermal and mineralization events. This study, therefore, provides crucial age data to support future mineral exploration in the district.
Digitalization, Street‐Level Bureaucracy and Welfare Users' Experiences
Authors: Hansen Hans‐Tore , Lundberg Kjetil , Syltevik Liv Johanne 
Published: December 2016

AbstractInternet/web‐based forms of communication have increasingly been implemented by welfare agencies. However, there have been few studies of the experiences of welfare service users and the consequences of new technology for welfare service users. To what extent is the new technology adopted by the Norwegian Welfare and Labour Organization (NAV) used, and how do the users apply and experience the new possibilities? Do screen‐to‐screen encounters replace face‐to‐face encounters, and is this trend affected by age, gender, education or type of benefit? To answer these questions, we combine survey data, short‐term fieldwork in welfare reception areas and qualitative interviews with people receiving health and work‐related benefits. Our study indicates that screen‐to‐screen interaction in general does not replace face‐to‐face encounters, as many face‐to‐face encounters are related to screen communication. However, digital competence combined with life circumstances appears to be the source of a new divide between welfare service users.
The EFSUMB website, a great source for ultrasound information and education
Authors: Dietrich Christoph F. , Rudd Lynne , Saftiou Adrian , Gilja Odd Helge 
Published: December 2016

The aim of this updated EFSUMB-website guide is to introduce readers to EFSUMB’s wide ranging activities. The most recent are the guidelines on interventional ultrasound and intestinal ultrasound and updated CEUS Non-Liver and Elastography Liver Guidelines which can be freely downloaded. Hosting eBooks on our website is another new departure, most importantly the EFSUMB Course Book on Ultrasound available in a second edition as an eReader and an online Student Edition of the ECB. EFSUMB has been active with updating Guidelines; those mentioned above have all been revised or written in thelast two years. Webinars have been introduced and participation is possible online but can be reviewed later along with recent recordings of Euroson Schools. The EFSUMB Newsletter in the EJU promotes our activities and topical articles intended to reach all our members with the online version hosted on our website. The Case of the Month continues to be one of EFSUMB’s most visited sites and in the last few years has been translated into 14 different languages including Chinese. In conclusion, this article aims to provide an updated guide to the website educational sites of the European Federation of Societies for Ultrasound in Medicine and Biology (EFSUMB).
Acoustic Radiation Force Impulse (ARFI) Elastography for non‑invasive evaluation of hepatic fibrosis in chronic hepatitis B and C patients: a systematic review and meta-analysis
Authors: Hu Xiangdong , Qiu Lanyan , Liu Dong , Qian Linxue 
Published: December 2016

Aim: The purpose of the study was to assess the effect of Acoustic Radiation Force Impulse (ARFI) elastography in the diagnosis of liver fibrosis in chronic hepatitis B and C patients through Meta-analysis. Material and methods: Four databases (PubMed, the Cochrane Library, WanFang data, and CNKI) were searched. The key words were: (“ARFI” or “acoustic radiation force impulse”) combined with “liver fibrosis” and (“chronic hepatitis” or “HBV HCV”). Heterogeneity (I2) was assessed, and its source was analyzed through meta-regression. Results: 21 articles with 2,691 patients were included. The compositeSe=0.79 (95% CI: 0.76-0.83) and Sp=0.86 (95% CI: 0.85-0.88). ARFI elastography showed a better ability to evaluate higherstage liver fibrosis and liver cirrhosis (F=3 and F=4, respectively). For F≥3, Se=0.84 (95% CI: 0.80-0.88, I2=61.37), Sp=0.90 (95% CI: 0.86-0.92, I2=65.10), and AUROC=0.94 (95% CI: 0.91-0.95). Se and Sp and AUROC of F=4 were 0.86 (95% CI: 0.80-0.91, I2=70.67), 0.84 (95% CI: 0.80–0.88, I2=78.94) and 0.91 (95% CI: 0.89-0.94), respectively. Besides, the combined RFI values indicate that CHC patients had higher ARFI values especially in the F3 stage (1.87 [95% CI: 1.67-2.06] and 2.31[95% CI: 2.09-2.52] for CHB and CHC, respectively). Conclusion: ARFI elastography is accurate and reliable in the diagnosis of CHB- and CHC-induced liver fibrosis and is especially suitable for the evaluation of stages F≥3 and F=4. CHC patients manifest higher ARFI values than CHB patients especially in the F3 stage.
Objectively Measured Physical Activity During Physical Education and School Recess and Their Associations With Academic Performance in Youth: The UP&DOWN Study
Authors: Esteban-Cornejo Irene , Martinez-Gomez David , Garcia-Cervantes Laura , Ortega Francisco B. , Delgado-Alfonso Alvaro , Castro-Piñero José , Veiga Oscar L. 
Published: December 2016

Background:This study examined the associations of objectively measured physical activity in Physical Education and recess with academic performance in youth.Methods:This cross-sectional study was conducted with 1,780 participants aged 6 to 18 years (863 girls). Physical activity was objectively measured by accelerometry and was also classified according to sex- and agespecific quartiles of physical activity intensities. Academic performance was assessed through school records.Results:Physical activity in physical education (PE) and recess was not associated with academic performance (β ranging from –0.038 to –0.003; all P > .05). Youth in the lowest quartile of physical activity in PE engaged in an average of 1.40 min of moderate-to-vigorous physical activity and those in the highest quartile engaged in 21.60 min (for recess: lowest quartile, 2.20 min; highest quartile, 11.15 min). There were no differences in academic performance between quartiles of physical activity in Physical Education and recess.Conclusions:Time spent at different physical activity intensities during PE and recess does not impair academic performance in youth.
A Physical Activity Intervention Program in School is Also Accompanied by Higher Leisure-Time Physical Activity: A Prospective Controlled 3-Year Study in 194 Prepubertal Children
Authors: Cronholm Felix , Rosengren Björn E. , Karlsson Caroline , Karlsson Magnus K. 
Published: December 2016

Background:The activity-stat theory infers that total physical activity (PA) in children is constant, independent of environmental interventions.Methods:We conducted a 3-year prospective population-based controlled PA intervention study including, at baseline, 7- to 9-year-old children (66 boys, 40 girls in the intervention and 50 boys, 38 girls in the control group). PA was increased in the intervention group from 60 to 200 minutes/week, while the controls maintained 60 minutes/week. We registered weekly duration of total PA and leisure-time PA and daily duration of sedentary activities, through questionnaires at baseline and 2 and 3 years after baseline.Results:Between intervention and control groups PA was similar before intervention start. After intervention start, total PA in both genders was increased during the entire period (P-values adjusted for age and Tanner stage at follow-up between 0.001 and 0.002). Duration of sedentary activities was unchanged with no group differences. Children in the intervention group changed their behavior so that they also achieved more leisure-time PA.Conclusions:A 3-year school-based PA intervention program in prepubertal children increases the duration of total PA without increasing the duration of sedentary activities, and the program seems to initiate more PA during leisure-time. Our results refute the activity-stat theory.
Azithromycin-Ciprofloxacin-Impregnated Urinary Catheters Avert Bacterial Colonization, Biofilm Formation, and Inflammation in a Murine Model of Foreign-Body-Associated Urinary Tract Infections Caused by Pseudomonas aeruginosa
Authors: Saini Hina , Vadekeetil Anitha , Chhibber Sanjay , Harjai Kusum 
Published: December 2016

ABSTRACTPseudomonas aeruginosais a multifaceted pathogen causing a variety of biofilm-mediated infections, including catheter-associated urinary tract infections (CAUTIs). The high prevalence of CAUTIs in hospitals, their clinical manifestations, such as urethritis, cystitis, pyelonephritis, meningitis, urosepsis, and death, and the associated economic challenges underscore the need for management of these infections. Biomaterial modification of urinary catheters with two drugs seems an interesting approach to combat CAUTIs by inhibiting biofilm. Previously, we demonstrated thein vitroefficacy of urinary catheters impregnated with azithromycin (AZM) and ciprofloxacin (CIP) againstP. aeruginosa. Here, we report how these coated catheters impact the course of CAUTI induced byP. aeruginosain a murine model. CAUTI was established in female LACA mice with uncoated or AZM-CIP-coated silicone implants in the bladder, followed by transurethral inoculation of 108CFU/ml of biofilm cells ofP. aeruginosaPAO1. AZM-CIP-coated implants (i) prevented biofilm formation on the implant's surface (P≤ 0.01), (ii) restricted bacterial colonization in the bladder and kidney (P< 0.0001), (iii) averted bacteriuria (P< 0.0001), and (iv) exhibited no major histopathological changes for 28 days in comparison to uncoated implants, which showed persistent CAUTI. Antibiotic implants also overcame implant-mediated inflammation, as characterized by trivial levels of inflammatory markers such as malondialdehyde (P< 0.001), myeloperoxidase (P< 0.05), reactive oxygen species (P≤ 0.001), and reactive nitrogen intermediates (P< 0.01) in comparison to those in uncoated implants. Further, AZM-CIP-coated implants showed immunomodulation by manipulating the release of inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and IL-10 to the benefit of the host. Overall, the study demonstrates long-termin vivoeffectiveness of AZM-CIP-impregnated catheters, which may possibly be a key to success in preventing CAUTIs.
Ciprofloxacin-Mediated Mutagenesis Is Suppressed by Subinhibitory Concentrations of Amikacin in Pseudomonas aeruginosa
Authors: Valencia Estela Ynés , Esposito Fernanda , Spira Beny , Blázquez Jesús , Galhardo Rodrigo S. 
Published: December 2016

ABSTRACT

Resistance to antibiotics is a global health problem. Activation of the SOS response, and the subsequent elevation in mutagenesis, contributes to the appearance of resistance mutations. Among currently used drugs, quinolones are the most potent inducers of the SOS response. In the present study, we show that amikacin inhibits ciprofloxacin-mediated SOS induction and mutagenesis in
Pseudomonas aeruginosa
.
PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal
Authors: Tada Tatsuya , Shrestha Shovita , Shimada Kayo , Ohara Hiroshi , Sherchand Jeevan B. , Pokhrel Bharat M. , Kirikae Teruo 
Published: December 2016

ABSTRACT

A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in an
Acinetobacter baumannii
clinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. The
k
cat
/

K
m

ratio of PER-8 for aztreonam was lower than that of PER-7, while the
k
cat
/

K
m

ratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surrounding
bla
PER-8
was
intI1 bla
PSE-1
qacEDI sulI
IS
CR1-bla
PER-8
gts sulI orfX
on a 100-kb plasmid.