bluuebunny/crossref_metadata_2025_split huggingface dataset:
GOOD FAITH IN CONTRACTS: IS THERE AN IMPLIED PROMISE TO ACT HONESTLY?
Authors: Carter J.W. , Courtney Wayne 
Published: August 2016

AbstractThis article critiques the model for implementation of good faith suggested by Leggatt J’s obiter comments in Yam Seng Pte Ltd v International Trade Corp Ltd. He considered that a general term of good faith may be implied as a matter of construction or as a factual implication under traditional implied terms rules; and that further terms might be implied as specific manifestations of the general term. These further terms would reflect “shared values and norms of behaviour”, including the “core value of honesty”. The article contends that the reasoning to support the general implication contradicts the proposition — accepted by Leggatt J — that good faith has not been recognised “as a duty implied by law”; and that, for several reasons, the analysis used to support implication on the basis of shared norms is flawed.
Methane emissions and growth performance of young Nellore bulls fed crude glycerine- v. fibre-based energy ingredients in low or high concentrate diets
Authors: LAGE J. F. , SAN VITO E. , REIS R. A. , DALLANTONIA E. E. , SIMONETTI L. R. , CARVALHO I. P. C. , BERNDT A. , CHIZZOTTI M. L. , FRIGUETTO R. T. S. , BERCHIELLI T. T. 
Published: August 2016

SUMMARYA total of 70 Nellore bulls (18 ± 3 months of age) were used to determine the effects of crude glycerine (CG) replacing starch- v. fibre-based energy ingredients in low (LC; 0·40 concentrate) or high concentrate (HC; 0·60 concentrate) – on a dry matter (DM) basis – on DM intake (DMI), methane emissions and growth. Ten bulls were slaughtered (reference group) to obtain the carcass gain (CrG). The 60 remaining bulls (374 ± 24·5 kg) were allocated to a 2 × 3 factorial arrangement (two concentrate levels, LC or HC; and three feeding regimes, FR). The FR were: CO – without CG and maize as an ingredient of concentrate; CGM – inclusion of CG (0·10 of DM) replacing maize in the concentrate; and CGSH – inclusion of CG (0·10 of DM) replacing soybean hulls (SH) in the concentrate. Bulls fed LC or HC had similar DMI (kg/d) and growth. The DMI and average daily gain (ADG) were similar among FR. Concentrate level and FR tended to interact for methane emissions (g) per kg DMI. Bulls fed CGM had a greater G : F (g CrG/kg DMI) than those fed CO or CGSH diets. Increasing dietary concentrate (0·40–0·60) did not affect intake, methane emissions, or growth. Inclusion of CG in diets to replace SH in LC diets tended to decrease methane emissions from animals. When CG replaces SH in the diets, CrG and G:F (g CrG/kg DMI) are decreased compared with bulls fed CGM.
A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells
Authors: Buo Atum M , Williams Mark S , Kerr Jaclyn P , Stains Joseph P 
Published: August 2016

AbstractWe report here a method for the use of poly-l-lysine (PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells (BMSCs) in culture and in situ, which are typically difficult to transduce. We show by fluorescence microscopy and flow cytometry that the addition of PLL to the viral-containing medium significantly increases the number of green fluorescence protein (GFP)-positive osteoblasts and BMSCs transduced with an enhanced GFP-expressing adenovirus. We also demonstrate that PLL can greatly enhance the adenoviral transduction of osteoblasts and osteocytes in situ in ex vivo tibia and calvaria, as well as in long bone fragments. In addition, we validate that PLL can improve routine adenoviral transduction studies by permitting the use of low multiplicities of infection to obtain the desired biologic effect. Ultimately, the use of PLL to facilitate adenoviral gene transfer in osteogenic cells can provide a cost-effective means of performing efficient gene transfer studies in the context of bone research.
The chromatin remodeler DDM1 promotes hybrid vigor by regulating salicylic acid metabolism
Authors: Zhang Qingzhu , Li Yanqiang , Xu Tao , Srivastava Ashish Kumar , Wang Dong , Zeng Liang , Yang Lan , He Li , Zhang Heng , Zheng Zhimin , Yang Dong-Lei , Zhao Cheng , Dong Juan , Gong Zhizhong , Liu Renyi , Zhu Jian-Kang 
Published: August 2016

AbstractIn plants, hybrid vigor is influenced by genetic and epigenetic mechanisms; however, the molecular pathways are poorly understood. We investigated the potential contributions of epigenetic regulators to heterosis in Arabidposis and found that the chromatin remodeler DECREASED DNA METHYLATION 1 (DDM1) affects early seedling growth heterosis in Col/C24 hybrids. ddm1 mutants showed impaired heterosis and increased expression of non-additively expressed genes related to salicylic acid metabolism. Interestingly, our data suggest that salicylic acid is a hormetic regulator of seedling growth heterosis, and that hybrid vigor arises from crosses that produce optimal salicylic acid levels. Although DNA methylation failed to correlate with differential non-additively expressed gene expression, we uncovered DDM1 as an epigenetic link between salicylic acid metabolism and heterosis, and propose that the endogenous salicylic acid levels of parental plants can be used to predict the heterotic outcome. Salicylic acid protects plants from pathogens and abiotic stress. Thus, our findings suggest that stress-induced hormesis, which has been associated with increased longevity in other organisms, may underlie specific hybrid vigor traits.
Ferromagnetic particles as magnetic resonance imaging temperature sensors
Authors: Hankiewicz J. H. , Celinski Z. , Stupic K. F. , Anderson N. R. , Camley R. E. 
Published: August 2016

AbstractMagnetic resonance imaging is an important technique for identifying different types of tissues in a body or spatial information about composite materials. Because temperature is a fundamental parameter reflecting the biological status of the body and individual tissues, it would be helpful to have temperature maps superimposed on spatial maps. Here we show that small ferromagnetic particles with a strong temperature-dependent magnetization, can be used to produce temperature-dependent images in magnetic resonance imaging with an accuracy of about 1 °C. This technique, when further developed, could be used to identify inflammation or tumours, or to obtain spatial maps of temperature in various medical interventional procedures such as hyperthermia and thermal ablation. This method could also be used to determine temperature profiles inside nonmetallic composite materials.
Balanced translocation linked to psychiatric disorder, glutamate, and cortical structure/function
Authors: Thomson Pippa A , Duff Barbara , Blackwood Douglas H R , Romaniuk Liana , Watson Andrew , Whalley Heather C , Li Xiang , Dauvermann Maria R , Moorhead T William J , Bois Catherine , Ryan Niamh M , Redpath Holly , Hall Lynsey , Morris Stewart W , van Beek Edwin J R , Roberts Neil , Porteous David J , St. Clair David , Whitcher Brandon , Dunlop John , Brandon Nicholas J , Hughes Zoë A , Hall Jeremy , McIntosh Andrew , Lawrie Stephen M 
Published: August 2016

AbstractRare genetic variants of large effect can help elucidate the pathophysiology of brain disorders. Here we expand the clinical and genetic analyses of a family with a (1;11)(q42;q14.3) translocation multiply affected by major psychiatric illness and test the effect of the translocation on the structure and function of prefrontal, and temporal brain regions. The translocation showed significant linkage (LOD score 6.1) with a clinical phenotype that included schizophrenia, schizoaffective disorder, bipolar disorder, and recurrent major depressive disorder. Translocation carriers showed reduced cortical thickness in the left temporal lobe, which correlated with general psychopathology and positive psychotic symptom severity. They showed reduced gyrification in prefrontal cortex, which correlated with general psychopathology severity. Translocation carriers also showed significantly increased activation in the caudate nucleus on increasing verbal working memory load, as well as statistically significant reductions in the right dorsolateral prefrontal cortex glutamate concentrations. These findings confirm that the t(1;11) translocation is associated with a significantly increased risk of major psychiatric disorder and suggest a general vulnerability to psychopathology through altered cortical structure and function, and decreased glutamate levels.
Learning strategies: a synthesis and conceptual model
Authors: Hattie John A C , Donoghue Gregory M 
Published: August 2016

AbstractThe purpose of this article is to explore a model of learning that proposes that various learning strategies are powerful at certain stages in the learning cycle. The model describes three inputs and outcomes (skill, will and thrill), success criteria, three phases of learning (surface, deep and transfer) and an acquiring and consolidation phase within each of the surface and deep phases. A synthesis of 228 meta-analyses led to the identification of the most effective strategies. The results indicate that there is a subset of strategies that are effective, but this effectiveness depends on the phase of the model in which they are implemented. Further, it is best not to run separate sessions on learning strategies but to embed the various strategies within the content of the subject, to be clearer about developing both surface and deep learning, and promoting their associated optimal strategies and to teach the skills of transfer of learning. The article concludes with a discussion of questions raised by the model that need further research.
Prostate-specific extracellular vesicles as a novel biomarker in human prostate cancer
Authors: Park Yong Hyun , Shin Hyun Woo , Jung Ae Ryang , Kwon Oh Sung , Choi Yeong-Jin , Park Jaesung , Lee Ji Youl 
Published: August 2016

AbstractExtracellular vesicles (EVs) may play an important role in cancer development and progression. We aimed to investigate the prognostic potential of prostate-specific EVs in prostate cancer (PCa) patients. Plasma and prostate tissue were collected from patients who underwent surgery for PCa (n = 82) or benign prostatic hyperplasia (BPH, n = 28). To analyze the quantity of EVs in prostate, we performed transmission electron microscopy (TEM), immuno-TEM with CD63 and prostate-specific membrane antigen (PSMA), and immunofluorescence staining. After EV isolation from plasma, CD63 and PSMA concentration was measured using ELISA kits. PSMA-positive areas in prostate differed in patients with BPH, and low-, intermediate-, and high-risk PCa (2.4, 8.2, 17.5, 26.5%, p < 0.001). Plasma PSMA-positive EV concentration differed in patients with BPH, and low-, intermediate-, and high-risk PCa (21.9, 43.4, 49.2, 59.9 ng/mL, p < 0.001), and ROC curve analysis indicated that plasma PSMA-positive EV concentration differentiated PCa from BPH (AUC 0.943). Patients with lower plasma PSMA-positive EV concentration had greater prostate volume (50.2 vs. 33.4 cc, p < 0.001) and lower pathologic Gleason score (p = 0.025). During the median follow-up of 18 months, patients with lower plasma PSMA-positive EV concentration tended to have a lower risk of biochemical failure than those with higher levels of prostate-specific EVs (p = 0.085).
No evidence for spectral jamming avoidance in echolocation behavior of foraging pipistrelle bats
Authors: Götze Simone , Koblitz Jens C. , Denzinger Annette , Schnitzler Hans-Ulrich 
Published: August 2016

AbstractFrequency shifts in signals of bats flying near conspecifics have been interpreted as a spectral jamming avoidance response (JAR). However, several prerequisites supporting a JAR hypothesis have not been controlled for in previous studies. We recorded flight and echolocation behavior of foraging Pipistrellus pipistrellus while flying alone and with a conspecific and tested whether frequency changes were due to a spectral JAR with an increased frequency difference, or whether changes could be explained by other reactions. P. pipistrellus reacted to conspecifics with a reduction of sound duration and often also pulse interval, accompanied by an increase in terminal frequency. This reaction is typical of behavioral situations where targets of interest have captured the bat’s attention and initiated a more detailed exploration. All observed frequency changes were predicted by the attention reaction alone, and do not support the JAR hypothesis of increased frequency separation. Reaction distances of 1–11 m suggest that the attention response may be elicited either by detection of the conspecific by short range active echolocation or by long range passive acoustic detection of echolocation calls.
Novel Poly(3-hydroxybutyrate-g-vinyl alcohol) Polyurethane Scaffold for Tissue Engineering
Authors: Reyes Adriana Pétriz , Martínez Torres Ataúlfo , Carreón Castro Ma. del Pilar , Rodríguez Talavera José Rogelio , Muñoz Susana Vargas , Aguilar Víctor Manuel Velázquez , Torres Maykel González 
Published: August 2016

AbstractThe design of new synthetic grafted poly(3-hydroxybutyrate) as composite 3D-scaffolds is a convenient alternative for tissue engineering applications. The chemically modified poly(3-hydroxybutyrate) is receiving increasing attention for use as biomimetic copolymers for cell growth. As of yet, these copolymers cannot be used efficiently because of the lack of good mechanical properties. Here, we address this challenge, preparing a composite-scaffold of grafted poly(3-hydroxybutyrate) polyurethane for the first time. However, it is unclear if the composite structure and morphology can also offer a biological application. We obtained the polyurethane by mixing a polyester hydroxylated resin with polyisocyanate and the modified polyhydroxyalkanoates. The results show that the poly(3-hydroxybutyrate) grafted with poly(vinyl alcohol) can be successfully used as a chain extender to form a chemically-crosslinked thermosetting polymer. Furthermore, we show a proposal for the mechanism of the polyurethane synthesis, the analysis of its morphology and the ability of the scaffolds for growing mammalian cells. We demonstrated that astrocytes isolated from mouse cerebellum and HEK293 can be cultured in the prepared material and express efficiently fluorescent proteins by adenoviral transduction. We also tested the metabolism of Ca2+ to obtain evidence of the biological activity.
A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans
Authors: Wang Yu , Wei Dongsheng , Zhu Xiangyang , Pan Jiao , Zhang Ping , Huo Liang , Zhu Xudong 
Published: August 2016

AbstractLoss-of-function mutagenesis is an important tool used to characterize gene functions and the CRISPR-Cas9 system is a powerful method for performing targeted mutagenesis in organisms that present low recombination frequencies, such as the serotype D strains of Cryptococcus neoformans. However, when the CRISPR-Cas9 system persists in the host cells, off-target effects and Cas9 cytotoxicity may occur, which might block subsequent genetic manipulation. Here, we report a method of spontaneously eliminating the CRISPR-Cas9 system without impairing its robust editing function. We successfully expressed single guide RNA under the driver of an endogenous U6 promoter and the human codon-optimized Cas9 endonuclease with an ACT1 promoter. This system can effectively generate an indel mutation and efficiently perform targeted gene disruption via homology-directed repair by electroporation in yeast. We then demonstrated the spontaneous elimination of the system via a cis arrangement of the CRISPR-Cas9 expression cassettes to the recombination construct. After a system-mediated double crossover, the CRISPR-Cas9 cassettes were cleaved and degraded, which was validated by Southern blotting. This ‘suicide’ CRISPR-Cas9 system enables the validation of gene functions by subsequent complementation and has the potential to minimize off-target effects. Thus, this technique has the potential for use in functional genomics studies of C. neoformans.
An atomic symmetry-controlled thermal switch
Authors: Manzano Daniel , Kyoseva Elica 
Published: August 2016

AbstractWe propose a simple diatomic system trapped inside an optical cavity to control the energy flow between two thermal baths. Through the action of the baths the system is driven to a non-equilibrium steady state. Using the Large Deviation theory we show that the number of photons flowing between the two baths is dramatically different depending on the symmetry of the atomic states. Here we present a deterministic scheme to prepare symmetric and antisymmetric atomic states with the use of external driving fields, thus implementing an atomic control switch for the energy flow.
A Nanoscale Interface Promoting Molecular and Functional Differentiation of Neural Cells
Authors: Posati Tamara , Pistone Assunta , Saracino Emanuela , Formaggio Francesco , Mola Maria Grazia , Troni Elisabetta , Sagnella Anna , Nocchetti Morena , Barbalinardo Marianna , Valle Francesco , Bonetti Simone , Caprini Marco , Nicchia Grazia Paola , Zamboni Roberto , Muccini Michele , Benfenati Valentina 
Published: August 2016

AbstractPotassium channels and aquaporins expressed by astrocytes are key players in the maintenance of cerebral homeostasis and in brain pathophysiologies. One major challenge in the study of astrocyte membrane channels in vitro, is that their expression pattern does not resemble the one observed in vivo. Nanostructured interfaces represent a significant resource to control the cellular behaviour and functionalities at micro and nanoscale as well as to generate novel and more reliable models to study astrocytes in vitro. However, the potential of nanotechnologies in the manipulation of astrocytes ion channels and aquaporins has never been previously reported. Hydrotalcite-like compounds (HTlc) are layered materials with increasing potential as biocompatible nanoscale interface. Here, we evaluate the effect of the interaction of HTlc nanoparticles films with primary rat neocortical astrocytes. We show that HTlc films are biocompatible and do not promote gliotic reaction, while favouring astrocytes differentiation by induction of F-actin fibre alignment and vinculin polarization. Western Blot, Immunofluorescence and patch-clamp revealed that differentiation was accompanied by molecular and functional up-regulation of both inward rectifying potassium channel Kir 4.1 and aquaporin 4, AQP4. The reported results pave the way to engineering novel in vitro models to study astrocytes in a in vivo like condition.
Band gap bowing in NixMg1−xO
Authors: Niedermeier Christian A. , Råsander Mikael , Rhode Sneha , Kachkanov Vyacheslav , Zou Bin , Alford Neil , Moram Michelle A. 
Published: August 2016

AbstractEpitaxial transparent oxide NixMg1−xO (0 ≤ x ≤ 1) thin films were grown on MgO(100) substrates by pulsed laser deposition. High-resolution synchrotron X-ray diffraction and high-resolution transmission electron microscopy analysis indicate that the thin films are compositionally and structurally homogeneous, forming a completely miscible solid solution. Nevertheless, the composition dependence of the NixMg1−xO optical band gap shows a strong non-parabolic bowing with a discontinuity at dilute NiO concentrations of x < 0.037. Density functional calculations of the NixMg1−xO band structure and the density of states demonstrate that deep Ni 3d levels are introduced into the MgO band gap, which significantly reduce the fundamental gap as confirmed by optical absorption spectra. These states broaden into a Ni 3d-derived conduction band for x > 0.074 and account for the anomalously large band gap narrowing in the NixMg1−xO solid solution system.
A Versatile and Scalable Approach toward Robust Superhydrophobic Porous Materials with Excellent Absorbency and Flame Retardancy
Authors: Ruan Changping , Shen Mengxia , Ren Xiaoyan , Ai Kelong , Lu Lehui 
Published: August 2016

AbstractThe frequent oil spillages and the industrial discharge of organic contaminants have not only created severe environmental and ecological crises, but also cause a risk of fire and explosion. These environmental and safety issues emphasize the urgent need for materials that possess superior sorption capability and less flammability and thus can effectively and safely clean up the floating oils and water-insoluble organic compounds. Here we present the successful hydrophobic modification of the flame retardant melamine sponge with a commercial fluorosilicone, by using a facile one-step solvent-free approach and demonstrate that the resultant superhydrophobic sponge not only exhibits extraordinary absorption efficiency (including high capacity, superior selectivity, good recyclability, and simple recycling routes), but also retains excellent flame retardancy and robust stability. In comparison to conventional methods, which usually utilize massive organic solvents, the present approach does not involve any complicated process or sophisticated equipment nor generates any waste liquids, and thus is a more labor-saving, environment-friendly, energy-efficient and cost-effective strategy for the hydrophobic modification. Taking into account the critical role of hydrophobic porous materials, especially in the field of environmental remediation, the approach presented herein would be highly valuable for environmental remediation and industrial applications.
Identification of IL6 as a susceptibility gene for major depressive disorder
Authors: Zhang Chen , Wu Zhiguo , Zhao Guoqing , Wang Fan , Fang Yiru 
Published: August 2016

AbstractOur previous work implied that interleukin 6 (IL6) may be a biological marker for major depressive disorder (MDD). In this study, we performed a comprehensive genetic study to determine the association between the gene encoding IL6 (IL6) and MDD in Han Chinese. There were 50 drug-naïve MDD patients and 50 healthy controls undergoing an mRNA expression study. A sample of 772 patients with MDD and 759 healthy controls were used for genetic analysis. Next, we performed an eQTL analysis to identify whether risk SNP(s) is associated with IL6 expression in brain. Our results showed that patients with MDD have higher levels of IL6 than healthy controls (P = 0.008). The SNP rs1800797 has a significant association with MDD (P = 0.01) in a dominant model. The eQTL analysis showed a marginally significant association between the rs1800797 and IL6 expression in the frontal cortex (P = 0.087). Our preliminary findings are suggestive of an association between rs1800797 and the risk of MDD. Further investigations are required to evaluate this association in larger samples to increase statistical power and to examine the correlation between rs1800797 and IL6 methylation patterns.