bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appraising the Shelf Life of Farmed Cobia, Rachycentron canadum, by Application of a Quality Index Method
Authors: Fogaça Fabíola Helena dos Santos , Gonzaga Junior Marcondes Agostinho , Vieira Sidely Gil Alves , Araujo Thais Danyelle Santos , Farias Emanuel Airton , Ferreira‐Bravo Irani Alves , Silva Thiago Fernandes Alves , Calvet Rodrigo Maciel , Pereira Alitiene Lemos Moura , Prentice‐Hernández Carlos 
Published: July 2016

AbstractFreshness is the main concern of seafood quality, and the principal method to evaluate seafood freshness is sensory evaluation. The aim of this work was to study the quality changes of cobia, Rachycentron canadum, under ice storage through sensory and physical analysis as well as bacterial counting of specific spoilage organisms (SSOs). In particular, the utilization of a quality index method (QIM) scheme was proposed. Samples stored for 0–30 d were analyzed with the QIM. Ten panelists observed and registered the changes occurring in the fish starting on day zero and ending when the fish were spoiled. After developing the scheme, 11 sensory attributes were described in 23 points, which detailed the appearance of skin, eyes, abdomen, gills, and flesh deterioration. The volatile nitrogen compound measurements and microbiological data of the SSOs determined a shelf‐life of 19 d for the samples. Sensory analysis showed a rejection point at 15.67 d of ice storage. The natural degradation of myofibrillar proteins and collagen was observed during ice storage. In conclusion, a shelf‐life of 15 d was defined for raw cobia stored on ice.
Risk factors, clinical presentation and prognosis of mixed candidaemia: a population‐based surveillance in Spain
Authors: Ramos Antonio , Romero Yolanda , Sánchez‐Romero Isabel , Fortún Jesús , Paño José Ramón , Pemán Javier , Gurguí Mercè , Rodríguez‐Baño Jesús , Padilla Belén 
Published: July 2016

SummaryThe low incidence of mixed candidaemia (MC) may have precluded a better knowledge of its clinical presentation. The aim of the study was to analyse the risk factors, clinical presentation and prognosis of MC episodes. A comparison between MC and monomicrobial candidaemia within a prospective programme on candidaemia was performed in 29 hospitals between April 2010 and May 2011. In fifteen episodes of candidaemia corresponding to 15 patients, out of 752, two species of Candida (1.9%) were isolated. MC was more frequent in patients with HIV infection (12%, P = 0.038) and those admitted due to extensive burns (23%, P = 0.012). The Candida species most frequently identified in MC were C. albicans 12 patients (40%), C. glabrata seven patients (23.3%) and C. parapsilosis six patients (20%). Early mortality was higher (nine patients, 60%) in patients with MC than in patients with MMC (223 patients, 30.3%, P = 0.046). In conclusion, MC was was independently associated with increased mortality even after considering other prognostic factors. MC is an infrequent event that is more common in HIV infection and in patients suffering from burns, and is associated with increased mortality.
Landscape genomics reveals altered genome wide diversity within revegetated stands of Eucalyptus microcarpa (Grey Box)
Authors: Jordan Rebecca , Dillon Shannon K. , Prober Suzanne M. , Hoffmann Ary A. 
Published: July 2016

Summary

In order to contribute to evolutionary resilience and adaptive potential in highly modified landscapes, revegetated areas should ideally reflect levels of genetic diversity within and across natural stands. Landscape genomic analyses enable such diversity patterns to be characterized at genome and chromosomal levels.

Landscape‐wide patterns of genomic diversity were assessed in Eucalyptus microcarpa, a dominant tree species widely used in revegetation in Southeastern Australia. Trees from small and large patches within large remnants, small isolated remnants and revegetation sites were assessed across the now highly fragmented distribution of this species using the DArTseq genomic approach.

Genomic diversity was similar within all three types of remnant patches analysed, although often significantly but only slightly lower in revegetation sites compared with natural remnants. Differences in diversity between stand types varied across chromosomes. Genomic differentiation was higher between small, isolated remnants, and among revegetated sites compared with natural stands.

We conclude that small remnants and revegetated sites of our E. microcarpa samples largely but not completely capture patterns in genomic diversity across the landscape. Genomic approaches provide a powerful tool for assessing restoration efforts across the landscape.
The auxin response factor MONOPTEROS controls meristem function and organogenesis in both the shoot and root through the direct regulation of PIN genes
Authors: Krogan Naden T. , Marcos Danielle , Weiner Aaron I. , Berleth Thomas 
Published: July 2016

Summary

The regulatory effect auxin has on its own transport is critical in numerous self‐organizing plant patterning processes. However, our understanding of the molecular mechanisms linking auxin signal transduction and auxin transport is still fragmentary, and important regulatory genes remain to be identified.

To track a key link between auxin signaling and auxin transport in development, we established an Arabidopsis thaliana genetic background in which fundamental patterning processes in both shoot and root were essentially abolished and the expression of PIN FORMED (PIN) auxin efflux facilitators was dramatically reduced.

In this background, we demonstrate that activating a steroid‐inducible variant of the auxin response factor (ARF) MONOPTEROS (MP) is sufficient to restore patterning and PIN gene expression. Further, we show that MP binds to distinct promoter elements of multiple genetically defined PIN genes.

Our work identifies a direct regulatory link between central, well‐characterized genes involved in auxin signal transduction and auxin transport. The steroid‐inducible MP system directly demonstrates the importance of this molecular link in multiple patterning events in embryos, shoots and roots, and provides novel options for interrogating the properties of self‐regulated auxin‐based patterning in planta.
The Danish Neck Disability Index: New Insights into Factor Structure, Generalizability, and Responsiveness
Authors: Lauridsen Henrik H. , O'Neill Lotte , Kongsted Alice , Hartvigsen Jan 
Published: July 2016

AbstractObjectiveTo (1) translate and culturally adapt and (2) determine the clinimetric properties of the Danish 8‐item Neck Disability Index (NDI‐8) in primary sector patients (PSPs) and secondary sector patients (SSPs).MethodsAnalyses included 326 patients with neck pain. Validity and reliability were assessed using a cross‐validation factor analytical design, hypothesis testing, internal consistency, measurement error, the smallest detectable change (SDC), and a generalizability study. Criterion and construct responsiveness, minimal important change (MIC), and floor and ceiling effects were determined.ResultsThe original Danish version of the NDI was not unidimensional. Omitting 2 items (pain, headache) revealed a 1‐factor structure (NDI‐8). Construct validity correctly predicted 88% of the hypotheses. Internal consistency (Cronbach's α) ranged between 0.88 and 0.89, and generalizability was high (Φ = 0.90). The SDC was 6.8 in PSPs and 5.9 in SSPs. Criterion responsiveness revealed poor areas under the curve for SSPs (0.58 to 0.64), and construct responsiveness was poor for PSPs (43% correctly predicted hypothesis). A floor effect of 26.5% was found in PSPs, with a change of 4 points representing the MIC.ConclusionThe modified Danish 8‐item NDI was unidimensional, had construct validity, and was reproducible with a large but acceptable measurement error. However, responsiveness was poor and a significant floor effect was found in PSPs. A change score representing the MIC is proposed for PSPs.
Plant‐based oral vaccines against zoonotic and non‐zoonotic diseases
Authors: Shahid Naila , Daniell Henry 
Published: July 2016

SummaryThe shared diseases between animals and humans are known as zoonotic diseases and spread infectious diseases among humans. Zoonotic diseases are not only a major burden to livestock industry but also threaten humans accounting for >60% cases of human illness. About 75% of emerging infectious diseases in humans have been reported to originate from zoonotic pathogens. Because antibiotics are frequently used to protect livestock from bacterial diseases, the development of antibiotic‐resistant strains of epidemic and zoonotic pathogens is now a major concern. Live attenuated and killed vaccines are the only option to control these infectious diseases and this approach has been used since 1890. However, major problems with this approach include high cost and injectable vaccines is impractical for >20 billion poultry animals or fish in aquaculture. Plants offer an attractive and affordable platform for vaccines against animal diseases because of their low cost, and they are free of attenuated pathogens and cold chain requirement. Therefore, several plant‐based vaccines against human and animals diseases have been developed recently that undergo clinical and regulatory approval. Plant‐based vaccines serve as ideal booster vaccines that could eliminate multiple boosters of attenuated bacteria or viruses, but requirement of injectable priming with adjuvant is a current limitation. So, new approaches like oral vaccines are needed to overcome this challenge. In this review, we discuss the progress made in plant‐based vaccines against zoonotic or other animal diseases and future challenges in advancing this field.
Biological characterization of Tomato leaf curl New Delhi virus from Spain
Authors: Ruiz L. , Simon A. , Velasco L. , Janssen D. 
Published: July 2016

Tomato leaf curl New Delhi virus (ToLCNDV; family Geminiviridae, genus Begomovirus) is an emerging virus in horticulture crops in Asia, and has recently been introduced in Spain, Tunisia and Italy. No betasatellite DNA was detected in infected tomato and zucchini squash samples from Spain, and agroinoculated viral DNA‐A and DNA‐B were sufficient to reproduce symptoms in plants of both crop species. Infected tomato and zucchini squash plants also served as inoculum sources for efficient transmission either mechanically or using Bemisia tabaci whiteflies. Cucumber, melon, watermelon, zucchini squash, tomato, eggplant and pepper, but not common bean, were readily infected using viruliferous whiteflies and expressed symptoms 8–15 days post‐inoculation. New full‐length sequences from zucchini squash and tomato indicated a high genetic homogeneity (>99% sequence identity) in the ToLCNDV populations in Spain, pointing to a single recent introduction event.
The Geometry of the Distance Coefficient in Gravity Equations in International Trade
Authors: Rauch Ferdinand 
Published: July 2016

AbstractGravity equations in trade imply that trade flows are proportional to the size of a country and inversely proportional to distance. This paper develops the analogy of gravity in physics with gravity in trade and provides geometric intuition for a large class of mathematical processes in two‐dimensional space for which these relationships would be expected. This model implies that distances between countries in empirical gravity estimations should be measured as weighted harmonic means of pairwise distances of local economic activity.
Shared responsibility: school nurses' experience of collaborating in school‐based interprofessional teams
Authors: Reuterswärd Marina , Hylander Ingrid 
Published: July 2016

BackgroundThe Swedish Education Act (2011) mandated a new combination of services to boost students' physical health, their mental health and special education through interprofessional pupil health and well‐being (PH) teams. For Swedish school nurses, providing these services presents new challenges.AimTo describe how Swedish school nurses experience their work and collaboration within the interprofessional PH teams.MethodsTwenty‐five school nurses (SNs) were interviewed in five focus groups. Content analysis was used to examine the data and to explore SNs' workplace characteristics by using the components of the sense of coherence (SOC) framework.ResultsSNs' experiences of work and collaboration within PH teams can be described using three domains: the expectations of others regarding SNs' roles, SNs' contributions to pupils' health and well‐being, and collaboration among SNs within PH teams. The results indicate a discrepancy between SNs' own experiences of their contribution and their experiences of other professionals' expectations regarding those contributions. Some duties were perceived as expected, comprehensible, manageable and meaningful, while other duties – though expected – were perceived as less meaningful, taking time away from school‐related matters. Other duties that were not explicitly expected – promoting general health and creating safety zones for pupils, teachers and parents, for example – were nonetheless perceived as meaningful. Collaboration within PH teams was considered meaningful, comprehensible and manageable only if the objectives of the team meetings were clear, if other professionals were available and if professional roles on the team were clearly communicated.ConclusionThe SNs reported a lack of clarity regarding their role in PH and its implementation in schools, indicating that professionals in PH teams need to discuss collaboration so as to find their niche given the new conditions. SOC theory emerged as a useful framework for discussing concrete work‐related factors in the school environment.
Interprofessional simulation to improve patient participation in transitional care
Authors: Dyrstad Dagrunn Nåden , Storm Marianne 
Published: July 2016

BackgroundEducating and training healthcare professionals is known to improve the quality of transitional care for older adults. Arranging interprofessional meetings for healthcare professionals might be useful to improve patient participation skills in transitional care.AimTo describe the learning activities used in The Meeting Point programme, focusing on patient participation in transitional care, and assess whether they increase healthcare professionals’ awareness of and competencies relating to patient participation in the transitional care of older patients.DesignData were collected as part of an educational intervention programme, The Meeting Point, including three seminars on ‘Patient participation in the transitional care of older patients’ and four follow‐up meetings. Participants were nurses, care assistants, doctors, physiotherapists, patient coordinators and administrative personnel from hospital, nursing homes and home‐based care services.MethodThe Meeting Point was organised around four pillars: introduction, teaching session, group work activity and plenary discussion. Qualitative data included log reports, summaries of meetings, notes from group work activities, and reports from participants and from follow‐up meetings.ResultsFeedback from participants shows that they were satisfied with meeting healthcare professionals from other units of care. A film scenario was perceived relevant for group work activity and useful in focusing participants’ attention to patient participation. Follow‐up meetings show that some nursing home wards, the emergency department and one medical ward at the hospital continued with ongoing work to improve quality of care. Efforts included implementation of an observational waiting room with comfortable chairs, planning for discharge in hospital admission, a daily patient flow registration system and motivational interviewing during admission to nursing home.ConclusionsThe description of the learning activities used at The Meeting Point seminars shows that they were useful to increase awareness of and competencies on patient participation in transitional care.
Effects of extended childbirth education by midwives on the childbirth fear of first‐time mothers: an RCT
Authors: Haapio Sari , Kaunonen Marja , Arffman Martti , Åstedt‐Kurki Päivi 
Published: July 2016

ObjectiveThis study evaluates how extended childbirth education intervention affects first‐time mothers’ fear of childbirth and its manifestation during pregnancy.MethodA randomised controlled trial was conducted. A total of 659 first‐time mothers were recruited before week 14 of gestation during the first ultrasound screening at the hospital's maternity outpatient clinic. The mothers were randomly assigned into an intervention group (n = 338) or a control group (n = 321). The control group received all available regular childbirth education. In addition, the intervention group received an enhanced 2‐hour childbirth education at the maternity hospital.Outcome measurements and statistical analysisThe objects of childbirth fears (childbirth‐related fear, fear for the mother's and the child's well‐being, fear related to Caesarean section) were the primary outcomes. The manifestations of childbirth fears (everyday life, stress symptoms and the wish to have a Caesarean section) were the secondary outcomes. These outcomes were measured over 34 weeks of gestation using two parts of the instrument ‘Feelings of Fear and Security Associated with Pregnancy and Childbirth’. Logistic and ordinal linear regression models were used to model the effect of the intervention on the outcomes.FindingsThe mothers in the intervention group had less childbirth‐related fear than those in the control group [odds ratio (OR) 0.58, 95% confidence level (CL) 0.38–0.88]. Also, fear influenced the mothers in the intervention group less in everyday life than it did the mothers in the control group [OR 0.64, 95% CL 0.44–0.94].ConclusionThe effectiveness of the intervention can be considered moderate: one of three objects and one of three manifestations of fears were reduced. The intervention proved most efficient in alleviating relatively limited objects of fears.
Health beliefs about lifestyle habits differ between patients and spouses 1 year after a cardiac event – a qualitative analysis based on the Health Belief Model
Authors: Köhler Anita Kärner , Nilsson Staffan , Jaarsma Tiny , Tingström Pia 
Published: July 2016

BackgroundSpousal concordance on risk factors and lifestyle habits exists and can partly be explained by patients' and spouses’ health beliefs and underuse of cardiac rehabilitation. However, there have been very few qualitative comparisons of health beliefs between patients and spouses after a cardiac event.AimTo examine and qualitatively compare the health beliefs of patients with coronary heart disease and their spouses about lifestyle habits, 1 year after the cardiac event.DesignExplorative and descriptive.MethodSemi‐structured focus group interviews were conducted with patients (n = 14) 1 year after a cardiac event, as well as individual interviews with spouses (n = 8). The transcriptions underwent a deductive qualitative content analysis, within the framework of the Health Belief Model.FindingsPatients' and spouses’ health beliefs about lifestyle habits qualitatively differed in most predetermined main analytical categories of the Health Belief Model. The patients relied more on their own capacity and the healthcare system than on collaboration with their spouses who instead emphasised the importance of mutual activities to establish lifestyle habits. The spouses therefore experienced problems with different family preferences compared to the patients' wishes. Moreover, only patients believed supervised exercise was beneficial for risk reduction of coronary heart disease and they related barriers for medication to a self‐healing body and a meaningless life without relatives and old habits. Patients and spouses agreed that despite the severity of illness, life was captured and that normalisation to a life as usual was possible.ConclusionThe patients' and spouses’ qualitatively different health beliefs regarding health‐related behaviours imply a new approach. Nurses and associated professionals need to follow‐up patients' and spouses’ in primary health care to support them in a tailored way, for example in problem‐based sessions. Recognition and understanding of their different views and otherness could lead to compromises and goals to work with.
Clinical behavior of intraocular teratoid medulloepithelioma in two‐related Quarter Horses
Authors: Monk Caroline S. , Craft William F. , Abbott Jeffrey R. , Farina Lisa L. , Reuss Sarah M. , Czerwinski Sarah L. , Brooks Dennis E. , Plummer Caryn E. 
Published: July 2016

AbstractThe objective of this paper is to describe clinical behavior, histopathologic features, and immunohistochemical staining of two‐related horses with intraocular teratoid medulloepithelioma. Two‐related Quarter Horses with similar intraocular masses presented to the UF‐CVM Comparative Ophthalmology Service for evaluation and treatment. The first horse, a 3‐year‐old gelding, had glaucoma and a cyst‐like mass in the anterior chamber. Enucleation was performed. Histopathology revealed a teratoid medulloepithelioma. The tumor was considered to be completely excised. Fifteen months later, the gelding presented with swelling of the enucleated orbit and local lymph nodes with deformation of the skull. Cytology revealed neuroectodermal neoplastic cells. Necropsy confirmed tumor metastasis. Six weeks later, a 9‐year‐old mare, a full sibling to the gelding, presented for examination. An infiltrative mass of the iris and ciliary body was found that extended into the anterior, posterior, and vitreal chambers. Uveitis was present, but secondary glaucoma was not noted. Enucleation was performed and the histopathologic diagnosis was also teratoid medulloepithelioma. The mare has had no recurrence to date, 2 years following enucleation. Metastasis of intraocular teratoid medulloepithelioma is possible. Staging is recommended in cases where the diagnosis of teratoid medulloepithelioma is confirmed. Surveillance of full siblings is recommended until more information regarding etiology is known.
Atypical associations between DI*A and DI*B alleles and the band 3‐Memphis mutation in Amazonian inhabitants
Authors: Sanguino E. C. , Tezza L. C. , Gomes K. S. , Neto J. P. , Albuquerque S. R. L. 
Published: July 2016

Background and ObjectivesThe Diego blood group system has a great anthropological and transfusion medicine importance, being associated with immediate, delayed haemolytic reactions and haemolytic disease of new born. Its 22 antigens are located on the integral band 3 glycoprotein, which is encoded by the SLC4A1 gene. We studied the frequency of DI*A, DI*B alleles, band 3 (166A) and band 3‐Memphis (A166G) in individuals from the Brazilian amazon. To investigate atypical associations between DI*A, DI*B alleles, band 3 and band 3‐Memphis.Materials and MethodsGenetic sequencing was performed with custom primers in 302 patients divided into four groups as follows: Group A (anaemic patients with erythrocyte membrane changes), Group B (anaemic patients without erythrocyte membrane changes), Group C (patients without anaemia with erythrocyte membrane changes) and Group D (healthy individuals).ResultsA frequency of 63 (20·9%) individuals having band 3‐Memphis was identified, of which 54 (17·9%) were heterozygous and 09 (3%) homozygous. The Diego system genotypic frequency was DI*A/DI*B (9·93%), DI*A/DI*A (0·90%), DI*B/DI*B (89·0%), of which the followed atypical associations were found: 14 (43·75%) DI*A allele associated with a homozygous 166A nucleotide polymorphism and 16 (2·82%) DI*B allele associated with homozygous 166G polymorphism; interestingly, this last one was found only in the B group.ConclusionsThese findings are important to understand the genetic associations between Diego blood group system and band 3 protein as well as to comprehend these atypical associations and their anthropological and haematological significance.