bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphism in ERCC1 confers susceptibility of coronary artery disease and severity of coronary artery atherosclerosis in a Chinese Han population
Authors: Zhang Shuai , Wang Xue-bin , Han Ya-di , Xiong Chen-ling , Zhou Ye , Wang Chen , Liu Ze-jin , Yang Na , Zheng Fang 
Published: July 2017

AbstractExcision repair cross-complementing 1 (ERCC1) gene encodes ERCC1 protein, which is mainly responsible for the repair of DNA damage in different diseases including coronary artery atherosclerosis by acting as a rate-limiting element in nucleotide excision repair (NER). Using a three-stage case-control study with 3037 coronary artery disease (CAD) patients and 3002 controls, we investigated associations of three single nucleotide polymorphisms (SNPs) with CAD risk and severity of coronary artery atherosclerosis in Chinese Han population. In the discovery set, the variant allele T of rs11615 was significantly associated with higher CAD risk (adjusted OR = 1.27, P = 0.006) and severity of coronary artery atherosclerosis (adjusted OR = 1.54, P = 0.003). These associations were more remarkable in the merged set (adjusted OR = 1.23, P = 8 × 10−6 for CAD risk; adjusted OR = 1.36, P = 4.3 × 10−5 for severity of coronary artery atherosclerosis). And the expression level of ERCC1 was significantly higher in CAD cases than controls. Multiplicative interactions among SNP rs11615, alcohol drinking, history of T2DM, and history of hyperlipidemia could increase 5.06-fold risk of CAD (P = 1.59 × 10−9). No significant association of rs2298881 and rs3212986 with CAD risk was identified. Taken together, SNP rs11615 in ERCC1 gene might confer susceptibility to CAD and severity of coronary atherosclerosis in a Chinese Han population.
Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus
Authors: Thomsen Mikkel T. , Wang Tobias , Milsom William K. , Bayley Mark 
Published: July 2017

AbstractFish regulate ventilation primarily by sensing O2-levels in the water and arterial blood. It is well established that this sensory process involves several steps, but the underlying mechanisms remain frustratingly elusive. Here we examine the effect of increasing lactate ions at constant pH on ventilation in a teleost; specifically the facultative air-breathing catfish Pangasianodon hypophthalmus. At lactate levels within the physiological range obtained by Na-Lactate injections (3.5 ± 0.8 to 10.9 ± 0.7 mmol L−1), gill ventilation increased in a dose-dependent manner to levels comparable to those elicited by NaCN injections (2.0 µmol kg−1), which induces a hypoxic response and higher than those observed in any level of ambient hypoxia (lowest PO2 = 20 mmHg). High lactate concentrations also stimulated air-breathing. Denervation of the first gill arch reduced the ventilatory response to lactate suggesting that part of the sensory mechanism for lactate is located at the first gill arch. However, since a residual response remained after this denervation, the other gill arches or extrabranchial locations must also be important for lactate sensing. We propose that lactate plays a role as a signalling molecule in the hypoxic ventilatory response in fish.
Covalent, Non-Covalent, Encapsulated Nanodrug Regulate the Fate of Intra- and Extracellular Trafficking: Impact on Cancer and Normal Cells
Authors: Kim Sang-Woo , Lee Yeon Kyung , Kim Sang-Hyun , Park Jun-Young , Lee Dong Un , Choi Jungil , Hong Jeong Hee , Kim Sanghyo , Khang Dongwoo 
Published: July 2017

AbstractDrugs need to be designed to access the designated intracellular organelle compartments in order to maximize anticancer efficacy. This study identified that covalently conjugated, non-covalent polyethylene glycol coated and encapsulated nanodrugs selectively influence drug uptake, the intracellular and extracellular trafficking of cancer cells. The types of nano conjugation modulated intracellular dynamics associated with differential impact on anti-cancer efficacy, but also induced differential cytotoxicity on cancer versus normal cells. In conclusion, this study demonstrated the importance of selecting the appropriate type of nano-conjugation for delivering organelle specific, active chemotherapeutic agents through controlled intracellular trafficking.
Uremia does not affect neointima formation in mice
Authors: Aarup Annemarie , Nielsen Carsten H. , Bisgaard Line S. , Bot Ilze , El-Ali Henrik H. , Kjaer Andreas , Nielsen Lars B. , Pedersen Tanja X. 
Published: July 2017

AbstractAtherosclerotic cardiovascular disease is a major complication of chronic kidney disease (CKD). CKD leads to uremia, which modulates the phenotype of aortic smooth muscle cells (SMCs). Phenotypic modulation of SMCs plays a key role in accelerating atherosclerosis. We investigated the hypothesis that uremia potentiates neointima formation in response to vascular injury in mice. Carotid wire injury was performed on C57BL/6 wt and apolipoprotein E knockout (Apoe−/−) mice two weeks after induction of uremia by 5/6 nephrectomy. Wire injury led to neointima formation and downregulation of genes encoding classical SMC markers (i.e., myocardin, α-smooth muscle actin, SM22-alpha, and smooth muscle myosin heavy chain) in both wt and Apoe−/− mice. Contrary to our expectations, uremia did not potentiate neointima formation, nor did it affect intimal lesion composition as judged from magnetic resonance imaging and histological analyses. Also, there was no effect of uremia on SMC marker gene expression in the injured carotid arteries, suggesting that there may be different effects of uremia on SMCs in different vascular beds. In conclusion, uremia does not accelerate neointima formation in response to wire injury of the carotid artery in mice.
Pupil adaptation corresponds to quantitative measures of autism traits in children
Authors: DiCriscio Antoinette Sabatino , Troiani Vanessa 
Published: July 2017

AbstractThe pupil is known to reflect a range of psychological and physiological variables, including cognitive effort, arousal, attention, and even learning. Within autism spectrum disorder (ASD), some work has used pupil physiology to successfully classify patients with or without autism. As we have come to understand the heterogeneity of ASD and other neurodevelopmental disorders, the relationship between quantitative traits and physiological markers has become increasingly more important, as this may lead us closer to the underlying biological basis for atypical responses and behaviors. We implemented a novel paradigm designed to capture patterns of pupil adaptation during sustained periods of dark and light conditions in a pediatric sample that varied in intellectual ability and clinical features. We also investigate the relationship between pupil metrics derived from this novel task and quantitative behavioral traits associated with the autism phenotype. We show that pupil metrics of constriction and dilation are distinct from baseline metrics. Pupil dilation metrics correlate with individual differences measured by the Social Responsiveness Scale (SRS), a quantitative measure of autism traits. These results suggest that using a novel, yet simple, paradigm can result in meaningful pupil metrics that correlate with individual differences in autism traits, as measured by the SRS.
3D semiconducting nanostructures via inverse lipid cubic phases
Authors: Burton M. R. , Lei C. , Staniec P. A. , Terrill N. J. , Squires A. M. , White N. M. , Nandhakumar Iris S. 
Published: July 2017

AbstractWell-ordered and highly interconnected 3D semiconducting nanostructures of bismuth sulphide were prepared from inverse cubic lipid mesophases. This route offers significant advantages in terms of mild conditions, ease of use and electrode architecture over other routes to nanomaterials synthesis for device applications. The resulting 3D bicontinous nanowire network films exhibited a single diamond topology of symmetry Fd3m (Q227) which was verified by Small angle X-ray scattering (SAXS) and Transmission electron microscopy (TEM) and holds great promise for potential applications in optoelectronics, photovoltaics and thermoelectrics.
Active Tuberculosis Incidence and Characteristics in Patients Treated with Tumor Necrosis Factor Antagonists According to Latent Tuberculosis Infection
Authors: Lee Eun Hye , Kang Young Ae , Leem Ah Young , Park Moo Suk , Kim Young Sam , Kim Se Kyu , Chang Joon , Kim Song Yee 
Published: July 2017

AbstractThis study aimed to determine the incidence and characteristics of active tuberculosis (TB) in patients treated with tumor necrosis factor (TNF) antagonists according to baseline latent tuberculosis infection (LTBI). Data were retrospectively obtained from 702 patients aged ≥20 years treated with TNF antagonists between November 2005 and June 2016 at Severance Hospital, a tertiary referral hospital in Seoul, South Korea. The interferon-gamma release assay (IGRA) with or without a tuberculin skin test (TST) was used to diagnose LTBI. Of the total of 702 patients, LTBI was diagnosed in 255 (36.3%) patients. 23.9% (168/702) had positive IGRA results, and 32.2% (165/512) had positive TST results. Five patients developed active TB after LTBI treatment, and 6 developed TB despite baseline negative LTBI results. After adjustment for age and sex, the standardized TB incidence ratio was 6.01 (95% CI 1.94–14.04) in the LTBI group and 5.14 (95% CI 1.88–11.18) in the baseline negative LTBI group. Clinicians should be aware of the risk of active TB in patients treated with TNF antagonists despite baseline negative LTBI screening results. Regular monitoring and serial tests should be considered during long-term TNF antagonist therapy, especially in intermediate to high TB burden country.
Altered visual cortical processing in a mouse model of MECP2 duplication syndrome
Authors: Zhang Dinghong , Yu Bin , Liu Jing , Jiang Weiqian , Xie Taorong , Zhang Ran , Tong Dali , Qiu Zilong , Yao Haishan 
Published: July 2017

AbstractAs an epigenetic modulator of gene expression, Methyl-CpG binding protein 2 (MeCP2) is essential for normal neurological function. Dysfunction of MeCP2 is associated with a variety of neurological disorders. MECP2 gene duplication in human causes neuropsychiatric symptoms such as mental retardation and autism. MeCP2 overexpression in mice results in neurobehavioural disorders, dendritic abnormalities, and synaptic defects. However, how gain of MeCP2 function influences cortical processing of sensory information remains unclear. In this study, we examined visual processing in a mouse model of MECP2 duplication syndrome (MECP2 Tg1 mouse) at 8 and 14 weeks, which were before and after the onset of behavioural symptoms, respectively. In vivo extracellular recordings from primary visual cortex (V1) showed that neurons in Tg1 mice at both adult ages preferred higher spatial frequencies (SFs) than those in wild-type (WT) littermate controls, and the semi-saturation contrasts of neurons were lower in Tg1 mice at 8 weeks but not at 14 weeks. Behavioural experiments showed that the performance for visual detection at high SFs and low contrasts was higher in MECP2 Tg1 mice. Thus, MeCP2 gain-of-function in mice leads to higher visual acuity and contrast sensitivity, both at the levels of cortical response and behavioural performance.
Genome-wide Analysis and Expression Divergence of the Trihelix family in Brassica Rapa: Insight into the Evolutionary Patterns in Plants
Authors: Wang Wenli , Wu Peng , Liu TongKong , Ren Haibo , Li Ying , Hou Xilin 
Published: July 2017

AbstractTrihelix gene family is an important transcription factor (TF) family involved in plants’ growth and development. This extensive study of trihelix genes from Arabidopsis thaliana to Brassica rapa could shed light on the evolution in plants and support crop breeding. In this study, a total of 52 trihelix genes were identified in B.rapa. Whole-genome annotation, molecular-evolution and gene-expression analyses of all known trihelix genes were conducted. By statistics of the number of trihelix genes in each species, we found the expansion of trihelix gene family started with angiosperm evolution. And SIP1 was more preferentially retained than other subgroups (GT-1, GT-2, GTγ, SH4), consistent with the gene dosage hypothesis. Then we investigated the evolutionary patterns, footprints and conservation of trihelix genes in selected plants. The putative trihelix proteins were highly conserved, but their expression patterns varied. Half of these genes were highly expressed in all the selected organs but some showed tissue-specific expression patterns. Furthermore, among six abiotic stresses (Cold, Heat, PEG, NaCl, ABA and GA), most trihelix genes were activated by salt and ABA treatment. In summary, the phylogenetic, evolution and expression analyses of trihelix gene family in B.rapa establish a solid foundation for future comprehensive functional analysis of BraTHs.
Autistic traits and positive psychotic experiences modulate the association of psychopathic tendencies with theory of mind in opposite directions
Authors: Gillespie Steven M. , Mitchell Ian J. , Abu-Akel Ahmad M. 
Published: July 2017

AbstractVarious clinical disorders, including psychopathy, and autism and schizophrenia spectrum disorders, have been linked with impairments in Theory of Mind (ToM). However, although these conditions can co-occur in the same individual, the effect of their inter-play on ToM abilities has not been investigated. Here we assessed ToM abilities in 55 healthy adults while performing a naturalistic ToM task, requiring participants to watch a short film and judge the actors’ mental states. The results reveal for the first time that autistic traits and positive psychotic experiences interact with psychopathic tendencies in opposite directions to predict ToM performance—the interaction of psychopathic tendencies with autism traits was associated with a decrement in performance, whereas the interaction of psychopathic tendencies and positive psychotic experiences was associated with improved performance. These effects were specific to cognitive rather than affective ToM. These results underscore the importance of the simultaneous assessment of these dimensions within clinical settings. Future research in these clinical populations may benefit by taking into account such individual differences.
Association of microRNAs with Argonaute proteins in the malaria mosquito Anopheles gambiae after blood ingestion
Authors: Fu Xiaonan , Dimopoulos George , Zhu Jinsong 
Published: July 2017

AbstractDrastic changes in gene expression occur after adult female mosquitoes take a blood meal and use the nutrients for egg maturation. A growing body of evidence indicates that microRNAs (miRNAs) contribute to this tightly controlled tissue- and stage-specific gene expression. To investigate the role of miRNAs, we monitored miRNA expression in the mosquito Anopheles gambiae during the 72-h period immediately after blood feeding. We also measured the association of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the functional status of individual miRNA species. Overall, 173 mature miRNAs were precipitated with Ago1 and Ago2, including 12 new miRNAs, the orthologs of which are found thus far only in other Anopheles species. Ago1 is the predominant carrier of miRNAs in Anopheles gambiae. The abundance and Ago loading of most of the mature miRNAs were relatively stable after blood ingestion. However, miRNAs of the miR-309/286/2944 cluster were considerably upregulated after blood feeding. Injection of the specific antagomir for miR-309 resulted in smaller developing oocytes and ultimately fewer eggs. In addition, the Ago association of some miRNAs was not proportional to their cellular abundance, suggesting that integration of miRNAs into the Ago complexes is regulated by additional mechanisms.
Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
Authors: Witek Małgorzata A. , Aufforth Rachel D. , Wang Hong , Kamande Joyce W. , Jackson Joshua M. , Pullagurla Swathi R. , Hupert Mateusz L. , Usary Jerry , Wysham Weiya Z. , Hilliard Dawud , Montgomery Stephanie , Bae-Jump Victoria , Carey Lisa A. , Gehrig Paola A. , Milowsky Matthew I. , Perou Charles M. , Soper John T. , Whang Young E. , Yeh Jen Jen , Martin George , Soper Steven A. 
Published: July 2017

AbstractCirculating tumor cells consist of phenotypically distinct subpopulations that originate from the tumor microenvironment. We report a circulating tumor cell dual selection assay that uses discrete microfluidics to select circulating tumor cell subpopulations from a single blood sample; circulating tumor cells expressing the established marker epithelial cell adhesion molecule and a new marker, fibroblast activation protein alpha, were evaluated. Both circulating tumor cell subpopulations were detected in metastatic ovarian, colorectal, prostate, breast, and pancreatic cancer patients and 90% of the isolated circulating tumor cells did not co-express both antigens. Clinical sensitivities of 100% showed substantial improvement compared to epithelial cell adhesion molecule selection alone. Owing to high purity (>80%) of the selected circulating tumor cells, molecular analysis of both circulating tumor cell subpopulations was carried out in bulk, including next generation sequencing, mutation analysis, and gene expression. Results suggested fibroblast activation protein alpha and epithelial cell adhesion molecule circulating tumor cells are distinct subpopulations and the use of these in concert can provide information needed to navigate through cancer disease management challenges.
Applying an Integrative Framework of Executive Function to Preschoolers With Specific Language Impairment
Authors: Kapa Leah L. , Plante Elena , Doubleday Kevin 
Published: July 2017

Purpose
The first goal of this research was to compare verbal and nonverbal executive function abilities between preschoolers with and without specific language impairment (SLI). The second goal was to assess the group differences on 4 executive function components in order to determine if the components may be hierarchically related as suggested within a developmental integrative framework of executive function.

Method
This study included 26 4- and 5-year-olds diagnosed with SLI and 26 typically developing age- and sex-matched peers. Participants were tested on verbal and nonverbal measures of sustained selective attention, working memory, inhibition, and shifting.

Results
The SLI group performed worse compared with typically developing children on both verbal and nonverbal measures of sustained selective attention and working memory, the verbal inhibition task, and the nonverbal shifting task. Comparisons of standardized group differences between executive function measures revealed a linear increase with the following order: working memory, inhibition, shifting, and sustained selective attention.

Conclusion
The pattern of results suggests that preschoolers with SLI have deficits in executive functioning compared with typical peers, and deficits are not limited to verbal tasks. A significant linear relationship between group differences across executive function components supports the possibility of a hierarchical relationship between executive function skills.
Tense Marking in the English Narrative Retells of Dual Language Preschoolers
Authors: Gusewski Svenja , Rojas Raúl 
Published: July 2017

Purpose
This longitudinal study investigated the emergence of English tense marking in young (Spanish–English) dual language learners (DLLs) over 4 consecutive academic semesters, addressing the need for longitudinal data on typical acquisition trajectories of English in DLL preschoolers.

Method
Language sample analysis was conducted on 139 English narrative retells elicited from 39 preschool-age (Spanish–English) DLLs (range = 39–65 months). Growth curve models captured within- and between-individual change in tense-marking accuracy over time. Tense-marking accuracy was indexed by the finite verb morphology composite and by 2 specifically developed adaptations. Individual tense markers were systematically described in terms of overall accuracy and specific error patterns.

Results
Tense-marking accuracy exhibited significant growth over time for each composite. Initially, irregular past-tense accuracy was higher than regular past-tense accuracy; over time, however, regular past-tense marking outpaced accuracy on irregular verbs.

Conclusions
These findings suggest that young DLLs can achieve high tense-marking accuracy assuming 2 years of immersive exposure to English. Monitoring the growth in tense-marking accuracy over time and considering productive tense-marking errors as partially correct more precisely captured the emergence of English tense marking in this population with highly variable expressive language skills.

Supplemental Materials

https://doi.org/10.23641/asha.5176942
Understanding evacuation and impact of a metro collision on ridership using large‐scale mobile phone data
Authors: Duan Zhengyu , Lei Zengxiang , Zhang Michael , Li Weifeng , Fang Jia , Li Jian 
Published: July 2017

As randomly occurring events, traffic accidents pose serious challenges to the collection of comprehensive data to understand how travellers respond to them and to quantify their impacts. The advent of mobile phones, with their wide spatial/temporal coverage and ubiquitous presence in metro areas, offers a new source of data to conduct such studies. In this study, the collision accident of Metro Line 10 in Shanghai, China on 27 September 2011 is carefully investigated based on data derived from anonymous mobile phone records. The evacuation process of the accident is studied, followed by an analysis of the impact of this accident on commuting in the city. After analysing 7 billion of mobile phone records for an 11‐day period, the authors find that the evacuation follows a two‐stage pattern. They then identify the commuters of Line 10 and study their commuting patterns in the day of accident and also in the subsequent days. They find that most of Line 10 commuters still preferred to use metro to complete their travels during the disruption period of Line 10, and returned to their typical commuting patterns immediately after Line 10 resumed service.
Rare Etiology of Acute Embolic Stroke in a 17-Year-Old Girl: Cardiac Papillary Fibroelastoma
Authors: Cooke Susan , Shillingford Michael , Ceithaml Eric , Chadha Tanya 
Published: July 2017

AbstractA cardiac papillary fibroelastoma is a benign, pedunculated tumor with strand-like projections arising from the endocardium. While often discovered incidentally, these tumors can embolize resulting in major neurologic events. There is a dearth of pediatric literature describing the necessity and optimal timing of surgical intervention for this rare primary cardiac tumor after a cerebrovascular event. We report an interesting case and the subsequent management dilemma in a teenage patient presenting with acute embolic strokes secondary to an aortic valve papillary fibroelastoma. Despite its rarity, a high index of suspicion should be maintained for such tumors in previously healthy children presenting with acute stroke.
Hook Plate Fixation for the Thumb Ulnar Collateral Ligament Fracture-Avulsion
Authors: Tabrizi Ali , Afshar Ahmadreza 
Published: July 2017

AbstractDuring a taekwondo competition, a 20-year-old female competitor injured her left thumb. There was pain, swelling, and tenderness at the ulnar side of the metacarpophalangeal joint. Plain radiography demonstrated an avulsion fracture with a small-size fragment at the base of the proximal phalanx. A mini hook plate was used to repair the lesion. The patient was satisfied with the results and returned to her sports activities after 2 months.