bluuebunny/crossref_metadata_2025_split huggingface dataset:
Linolenic acid improves oocyte developmental competence and decreases apoptosis ofin vitro-produced blastocysts in goat
Authors: Veshkini Arash , Khadem Ali Akbar , Mohammadi-Sangcheshmeh Abdollah , Alamouti Ali Asadi , Soleimani Masoud , Gastal Eduardo L. 
Published: November 2015

SummaryThe effects of α-linolenic acid (ALA) on developmental competence of oocytes in goats were evaluated in this study. Initially, the level of ALA in small and large antral follicles was determined to be in a range of 0.018–0.028 mg/ml (64.6–100.6 μM, respectively).In vitromaturation was performed in the presence of various concentrations (10, 50, 100, or 200 μM) of ALA. Cumulus expansion, meiotic maturation, levels of intracellular glutathione (GSH), embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) andin vitrofertilization (IVF), number of total and apoptotic cells in blastocyst, and expression ofBax, Bcl-2, and p53 genes in blastocyst cells were determined. Compared with the control, no improvement was observed in cumulus expansion in ALA-treated groups. At 50 μM concentration, ALA increased meiotic maturation rate but had no effect on GSH level. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher rate of blastocyst formation was observed, and these embryos had a higher total cell number and a lower apoptotic cell number. Expression analyses of genes in blastocysts revealed lesser transcript abundances forBaxgene, and higher transcript abundances forBcl-2gene in 50 μM ALA group. Expression ofp53gene was also less observed in ALA-treated blastocysts. Our results show that ALA treatment at 50 μM duringin vitromaturation (IVM) had a beneficial effect on maturation of goat oocytes and this, in turn, stimulated embryonic development and regulated apoptotic gene expression.
Vitamin D deficiency prevalence and risk factors among pregnant Chinese women
Authors: Yun Chunfeng , Chen Jing , He Yuna , Mao Deqian , Wang Rui , Zhang Yu , Yang Chun , Piao Jianhua , Yang Xiaoguang 
Published: November 2015

AbstractObjectiveTo evaluate vitamin D deficiency prevalence and risk factors among pregnant Chinese women.DesignA descriptive cross-sectional analysis.SettingChina National Nutrition and Health Survey (CNNHS) 2010–2013.SubjectsA total of 1985 healthy pregnant women participated. Possible predictors of vitamin D deficiency were evaluated via multiple logistic regression analyses.ResultsThe median serum 25-hydroxyvitamin D level was 15·5 (interquartile range 11·9–20·0, range 3·0–51·5) ng/ml, with 74·9 (95 % CI 73·0, 76·7) % of participants being vitamin D deficient (25-hydroxyvitamin D <20 ng/ml). According to the multivariate logistic regression analyses, vitamin D deficiency was positively correlated with Hui ethnicity (P=0·016), lack of vitamin D supplement use (P=0·021) and low ambient UVB level (P<0·001). In the autumn months, vitamin D deficiency was related to Hui ethnicity (P=0·012) and low ambient UVB level (P<0·001). In the winter months, vitamin D deficiency was correlated with younger age (P=0·050), later gestational age (P=0·035), higher pre-pregnancy BMI (P=0·019), low ambient UVB level (P<0·001) and lack of vitamin D supplement use (P=0·007).ConclusionsVitamin D deficiency is prevalent among pregnant Chinese women. Residing in areas with low ambient UVB levels increases the risk of vitamin D deficiency, especially for women experiencing advanced stages of gestation, for younger pregnant women and for women of Hui ethnicity; therefore, vitamin D supplementation and sensible sun exposure should be encouraged, especially in the winter months. Further studies must determine optimal vitamin D intake and sun exposure levels for maintaining sufficient vitamin D levels in pregnant Chinese women.
Seasonality and household diets in Ethiopia
Authors: Hirvonen Kalle , Taffesse Alemayehu Seyoum , Worku Hassen Ibrahim 
Published: November 2015

AbstractObjectiveTo revisit seasonality by assessing how household diets vary across agricultural seasons in rural and urban Ethiopia. The role of seasonality on the sources and intake of energy (per capita) and household dietary diversity score (HDDS) was analysed.DesignThe use of nationally representative household-level data collected each month over one year to study the seasonal changes in the sources and intake of energy and HDDS.SettingEleven regions of Ethiopia, including rural and urban settings.SubjectsTotal of 27 835 households were interviewed between July 2010 and July 2011 in all eleven regions of the country. On average each month saw 2300 household interviews, yielding nationally representative data for each calendar month.ResultsFor rural households, the mean daily per capita energy intake was 10 288 kJ (2459 kcal) in February (post-harvest period) and lower in the lean season: 9703 kJ (2319 kcal) in June (P<0·05) and 9552 kJ (2283 kcal) in July (P<0·001). HDDS for rural households was highest in February (6·73) and lowest in June (5·98; P<0·001) but high again in July (6·57). Urban energy intake was also lower in the lean season but HDDS varied less by season. Considerable seasonal variation was also found in energy sources in rural areas, less so in urban areas.ConclusionsHousehold diets in Ethiopia remain subjected to significant seasonal stress. HDDS and food security measured using energy intake do not always agree. Preferably, HDDS and energy intake data should be used together to assess food security.
Trends in food and beverage television brand appearances viewed by children and adolescents from 2009 to 2014 in the USA
Authors: Elsey James WB , Harris Jennifer L 
Published: November 2015

AbstractObjectivePublic health experts raise concerns about marketing unhealthy products to young people through television (TV) product placements. Coca-Cola brand appearances (product placements) reached a substantial child and adolescent audience in 2008, but additional brands now sponsor popular programming. We aimed to quantify child and adolescent exposure to food and beverage appearances since 2008.DesignIn 2015, we purchased Nielsen data on occurrences and child/adolescent exposure to food, beverage and restaurant brand appearances on US prime-time TV from 2009 to 2014, and analysed appearances by product category, company, brand and year. We compared exposure to appearances with exposure to traditional commercials for top brands.SettingNationally representative panel of approximately 20 000 TV-viewing households.SubjectsChildren (2–11 years) and adolescents (12–17 years).ResultsExposure to food and beverage brand appearances peaked in 2012 and declined through 2014. Whereas full-calorie soda brands dominated before 2012, other sugary drink and quick-serve restaurant brands contributed over one-third of appearances viewed by children in 2013 and 2014. Nine hundred and fifty-four companies had brand appearances from 2009 to 2014, but just four were responsible for over half of exposures: The Coca-Cola Company, Dr Pepper Snapple Group, PepsiCo and Starbucks. Approximately half were viewed on reality TV programmes and one sitcom. Each year from 2009 to 2013, brand appearance exposure exceeded traditional advertising exposure for at least one brand.ConclusionsDespite recent reductions in brand appearances viewed by young people, some unhealthy branded products continue to be marketed via this method. We suggest policy options to reduce child and adolescent exposure to such appearances.
Anatomy, Histochemistry, and Antifungal Activity of Anacardium humile (Anacardiaceae) Leaf
Authors: Royo Vanessa de A. , Mercadante-Simões Maria Olívia , Ribeiro Leonardo M. , Oliveira Dario A. de , Aguiar Marcela Magda R. , Costa Ellenhise R. , Ferreira Perácio Rafael B. 
Published: November 2015

AbstractLeaves of Anacardium humile are used in Brazilian traditional medicine for the treatment of intestinal disturbances and skin lesions. This study aimed to define leaf diagnostic structural characters, to propose a new method of phytochemical analysis of secretions, prospect flavonoids and alkaloids, and to evaluate their inhibitory activity on Candida albicans. Common anatomical, phytochemical, and microbiological methods were used. Leaves of Anacardium occidentale were used as a reference for the structural analyses. The main structural characters were closed vascular system, absence of ducts at the medulla, bilateral mesophyll, absence of bundle sheath extension, and secretory idioblasts at the xylem. The ducts present schizogenous origin, and secreting activity is restricted to the initial phases of leaf blade expansion. The proposed new phytochemical method is practical and inexpensive and has potential for wide application. The abundance of tannins and flavonoids is related to medicinal use. A single peak in high-performance liquid chromatography indicated the presence of a pure substance not previously reported. The extract had a strong inhibitory effect on C. albicans. The obtained results confirm the potential of A. humile for the prospection of new bioactive compounds.
Assessing Affective and Deliberative Decision-Making: Adaptation of the Columbia Card Task to Brazilian Portuguese
Authors: Kluwe-Schiavon Bruno , Sanvicente-Vieira Breno , Viola Thiago W. , Veiga Eduardo , Bortolotto Vanessa , Grassi-Oliveira Rodrigo 
Published: November 2015

AbstractThe ability to predict reward and punishment is essential for decision-making and the ability to learn about an ever-changing environment. Therefore, efforts have been made in understanding the mechanisms underlying decision-making, especially regarding how affective and deliberative processes interact with risk behavior. Objective: To adapt to Brazilian Portuguese the Columbia Card Task (CCT) and investigate affective and deliberative processes involved in decision-making. Methods: This study had two main phases: (1) a transcultural adaptation and (2) a pilot study. Results: The feedback manipulation among the three conditions of CCT had an effect on the risk-taking level (p< .005,ES= .201). In addition, the feedback manipulation among the three conditions of CCT had an effect on the information use at both the individual and group levels. Further, a linear regression suggested that the use of information, indicated by the advantageous level of the scenarios, predict the number of cards chosenR2= .029,p< .001, accounting for 17% of the variance. Conclusions: The Brazilian CCT performs well and is a versatile method for the assessment of affective and deliberative decision-making under risk according to different feedback manipulation scenarios. This study goes further, comparing electrodermal activity during hot and warm conditions and addressing an advantageous level index analysis to asses deliberative processing.
Spanish Version of the Avoidance-Endurance Questionnaire: Factor Structure and Psychometric Properties
Authors: Ruiz-Párraga Gema T. , López-Martínez Alicia E. , Rusu Adina C. , Hasenbring Monika I. 
Published: November 2015

AbstractTo analyze the factorial structure and psychometric properties of the Spanish adaptation of the AEQ, and to validate it by reporting relevant pain-related variables, which were not investigated in the original study. One hundred and fifty Spanish patients diagnosed with chronic back and neck pain were referred by physicians from different pain clinics in Spain; all the patients filled out the questionnaires at their clinic. A series of principal components analyses (PCA) was performed to develop the Spanish version of the AEQ. Reliability and validity were also calculated. The PCAs revealed five fear-avoidance scales (Kaiser-Meyer-Olkin measures were between .60 and .88, and Bartlett’s tests were significant, p < .01): the Depression scale (DS), Anxiety scale (AS), Catastrophizing scale (CS), Helplessness/hopelessness scale (HHS), and Avoidance of Social and Physical Activities scale (ASPAS), and three endurance-related responses scales: Pain Persistence Behaviour and Distraction scale (PPDS), Ignoring Pain scale (IPS), and Humor scale (HS). All the scales showed high internal consistency (α > .73) and suitable validity (p < .05). New results associated with pain-related cognitive/affective and behavioural responses are discussed. This instrument will probably help clinicians to identify Spanish patients at a high risk of chronicity and to develop treatments tailored to the different profiles in order to improve secondary and tertiary prevention in back and neck pain.
The 15-item Systemic Clinical Outcome and Routine Evaluation (SCORE-15) Scale: Portuguese Validation Studies
Authors: Vilaça Margarida , de Sousa Bruno , Stratton Peter , Relvas Ana Paula 
Published: November 2015

AbstractThis study reports on the validity of the 15-item Portuguese version of the Systemic Clinical Outcome Routine Evaluation (SCORE-15; Vilaça, Silva, & Relvas, 2014), a brief and comprehensive measure of family functioning. Previous studies with SCORE-15 show that this version replicates the three-factor solution found for the original English version: Family strengths, Family communication and Family difficulties. In addition to reviewing previous studies, this article analyses the discriminant, convergent and predictive validity of the Portuguese SCORE-15. To do so, the SCORE-15 was administered to family members attending systemic family or couple’s therapy at the start of the first and fourth sessions and also to a group of non-clinical individuals. Overall, data are reported from 618 participants, including 136 from families attending systemic therapy and 482 community family members. Comparisons of community and clinical samples (discriminant validity) showed statistically significant differences for the total scale and subscales (p < .001), with the community participants presenting healthier family functioning than the clinical ones. Analyses using SCORE-15 and the Quality of Life – adult version, another family measure applied simultaneously (convergent validity), indicate that both scales are significantly (p < .01) and moderately (r = –.47) correlated. Mean score analysis of SCORE-15’s therapeutic sensitivity to change (predictive validity) showed that only the Family communication subscale was sensitive to statistically significant improvement (p < .05) from session 1 to session 4, whereas the SCORE-15’s reliability change index points to its ability to detect clinical improvements (RCI = 14%).
A Multifactorial Conceptualization of Impulsivity
Authors: Knezevic-Budisin Bojana , Pedden Vanessa , White Andrew , Miller Carlin J. , Hoaken Peter N. S. 
Published: November 2015

Abstract. Despite the multiple phenotypic presentations of impulsivity, the underlying factor structure of the construct has yet to be settled. The aim of this study, with two multimethod, multisource datasets, was to further explore the multifactorial nature of impulsivity and propose a measure-selection approach. Unlike previous studies that relied on a single type of statistical analysis, the current study explored the relations between personality and behavioral measures of impulsivity utilizing exploratory factor analysis (EFA) and principal component analysis (PCA). Participants comprised two samples of young adults (n(study 1) = 175 and n(study 2) = 118) from separate communities in southwestern Ontario, Canada. Various facets of impulsivity were assessed including adult ADHD symptoms, planning and organizational skills, executive dysfunction, impulsive personality traits (i.e., sensation-seeking), risk-taking behavior, disinhibition, cognitive flexibility, and delay discounting. Both statistical analyses yielded two-factor models. The Dysexecutive Control factor reflected a tendency to act without thinking or planning, and difficulty focusing for a sustained period of time. The Reward-Seeking factor reflected a general need for excitement, and a preference for novel situations despite adverse consequences. For the purposes of standardized assessment of cognitive, emotional, and behavioral manifestations of impulsivity, trans-theoretical measure selection for research and clinical purposes is discussed.
FGFR1 inhibition in lung squamous cell carcinoma: questions and controversies
Authors: Weeden CE , Solomon B , Asselin-Labat M-L 
Published: November 2015

AbstractAlthough the incidence of lung cancer has decreased due to the reduction of tobacco use, lung cancer remains the leading cause of cancer-related death. Lung squamous cell carcinoma represents 30% of lung cancers and only recently have possible drug-targetable mutations been identified in this disease, including fibroblast growth factor receptor 1 (FGFR1) gene amplification and genetic alterations in the phosphoinositide-3 kinase pathway. These discoveries have generated a great interest in the clinic and the initiation of clinical trials using FGFR tyrosine kinase inhibitors to treat FGFR-altered lung cancers. However, preliminary results from these studies have shown that not all patients respond to therapy. Here we review current unresolved questions on the selection of patients for their recruitment in FGFR tyrosine kinase inhibitor trials, how FGFR inhibitors could be combined with other targeted therapies or immunotherapies to improve patient outcome, and how the current preclinical models can help address these questions.
CYP epoxygenase metabolites of docosahexaenoic acid protect HL-1 cardiac cells against LPS-induced cytotoxicity through SIRT1
Authors: Samokhvalov V , Jamieson K L , Vriend J , Quan S , Seubert J M 
Published: November 2015

AbstractBacterial LPS is an environmental toxin capable of promoting various cardiac complications. Current evidence suggests that LPS-induced myocardial dysfunction emerges as a consequence of compromised quality of cardiac mitochondria. Docosahexaenoic acid (DHA, 22:6n3) is an n-3 polyunsaturated fatty acid (PUFA), which produces a broad spectrum of intrinsic physiological effects including regulation of cell survival and death mechanisms. Although, numerous studies revealed fundamentally beneficial effects of DHA on cardiovascular system, it remains unknown whether these effects were produced by DHA or one of its possibly more potent metabolites. Emerging evidence indicates that cytochrome P450 (CYP) epoxygenase metabolites of DHA, epoxydocosapentaenoic acids (EDPs), produce more potent biological activity compared to its precursor DHA. In this study, we investigated whether DHA and its metabolite 19,20-EDP could protect HL-1 cardiac cells against LPS-induced cytotoxicity. We provide evidence that exogenously added or DHA-derived EDPs promote mitochondrial biogenesis and function in HL-1 cardiac cells. Our results illustrate the CYP epoxygenase metabolite of DHA, 19,20-EDP, confers extensive protection to HL-1 cardiac cells against LPS-induced cytotoxicity via activation of SIRT1.
p53 Configures the G2/M arrest response of nucleostemin-deficient cells
Authors: Huang G , Meng L , Tsai RYL 
Published: November 2015

AbstractNucleostemin (NS) protects the genome from replication-induced DNA damage and has an indispensable role in maintaining the continuous proliferation of both p53-wild-type and mutant cells. Yet, some outcomes of NS-deficient cells appear to be shaped by their p53 status, which stimulates conflicting claims on the role of p53 in executing the NS function. This disparity was conveniently attributed to the usual suspect of cell-type variations. To provide a definitive resolution, we investigated the interplay between NS and p53 in two pairs of isogenic cells, that is, genetically modified mouse embryonic fibroblast (MEF) cells and HCT116 human colon cancer cells. In MEF cells, p53 deletion further compromises rather than rescues the proliferative potential of NS-depleted cells without changing their G2/M arrest fate before prophase entry. The detrimental effect of p53 loss in NS-depleted MEF cells correlates with a dramatic increase of polyploid giant cells (PGCs) (up to 24%), which indicates aberrant mitosis. To determine how p53 shapes the response of cells to NS depletion at the molecular level, we showed that p53 turns on the expression of reprimo and MDM2 in NS-deficient MEF cells. In absence of p53, NS-deficient MEF cells exhibit increased levels of phosphorylated cdc2 (Y15) protein and cyclin B1. In cancer (HCT116) cells, NS loss leads to G2/M arrest under both p53wt and p53ko conditions and increases phosphorylated cdc2 more in p53ko than in p53wt cells, as it does in MEF cells. Unlike its effect in MEF cells, NS depletion decreases tumor growth and increases the expression of reprimo and cyclin B1 in a p53-independent manner in HCT116 cells. Our data indicate that the p53 status of NS-deficient cells orchestrates how they respond to G2/M arrest in a normal versus cancer cell distinct fashion.
Novel RNA- and FMRP-binding protein TRF2-S regulates axonal mRNA transport and presynaptic plasticity
Authors: Zhang Peisu , Abdelmohsen Kotb , Liu Yong , Tominaga-Yamanaka Kumiko , Yoon Je-Hyun , Ioannis Grammatikakis , Martindale Jennifer L. , Zhang Yongqing , Becker Kevin G. , Yang In Hong , Gorospe Myriam , Mattson Mark P. 
Published: November 2015

AbstractDespite considerable evidence that RNA-binding proteins (RBPs) regulate mRNA transport and local translation in dendrites, roles for axonal RBPs are poorly understood. Here we demonstrate that a non-telomeric isoform of telomere repeat-binding factor 2 (TRF2-S) is a novel RBP that regulates axonal plasticity. TRF2-S interacts directly with target mRNAs to facilitate their axonal delivery. The process is antagonized by fragile X mental retardation protein (FMRP). Distinct from the current RNA-binding model of FMRP, we show that FMRP occupies the GAR domain of TRF2-S protein to block the assembly of TRF2-S–mRNA complexes. Overexpressing TRF2-S and silencing FMRP promotes mRNA entry to axons and enhances axonal outgrowth and neurotransmitter release from presynaptic terminals. Our findings suggest a pivotal role for TRF2-S in an axonal mRNA localization pathway that enhances axon outgrowth and neurotransmitter release.
Single-cell analysis and stochastic modelling unveil large cell-to-cell variability in influenza A virus infection
Authors: Heldt Frank S. , Kupke Sascha Y. , Dorl Sebastian , Reichl Udo , Frensing Timo 
Published: November 2015

AbstractBiochemical reactions are subject to stochastic fluctuations that can give rise to cell-to-cell variability. Yet, how this variability affects viral infections, which themselves involve noisy reactions, remains largely elusive. Here we present single-cell experiments and stochastic simulations that reveal a large heterogeneity between influenza A virus (IAV)-infected cells. In particular, experimental data show that progeny virus titres range from 1 to 970 plaque-forming units and intracellular viral RNA (vRNA) levels span three orders of magnitude. Moreover, the segmentation of IAV genomes seems to increase the susceptibility of their replication to noise, since the level of different genome segments can vary substantially within a cell. In addition, simulations suggest that the abortion of virus entry and random degradation of vRNAs can result in a large fraction of non-productive cells after single-hit infection. These results challenge current beliefs that cell population measurements and deterministic simulations are an accurate representation of viral infections.