bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revolutionizing disease treatment through bioengineered probiotics and glucagon‐like peptide 1 (GLP‐1) based strategies: A path towards effective cures
Authors: Jain Smriti , Shukla Adarsh Kumar , Deepika  , Panwar Surbhi , Kumari Anita , Yadav Ashok Kumar , Kumar Ashwani 
Published: September 2024

AbstractHuman intestinal gut microbiota harbors complex and diverse microbes that play an important role in maintaining the homeostasis of the intestinal microenvironment in humans. The rise in mortality and morbidity rates among humans because of the increased incidence of food‐borne pathogens and the habits of individuals to eat junk food poses greater concerns and needs to be addressed. Bioengineering of probiotics has enabled the researchers to advance their research by developing probiotics with more functionalities. Moreover, GLP‐1 peptides which are incretin hormones have been shown to be more effective when combined with engineered probiotics. Various studies have shown its effectiveness in diabetic mice where human‐modified GLP‐1 produced long‐lasting benefits and research is going on to study its role in other diseases. The role of designer probiotics in treating and preventing diseases have been of much interest in recent times. However, the role of GLP‐1 peptides in treating diseases and their efficacy in combination with next‐gen biotherapeutics have received little attention. Thus, this review enlightens about the baseline knowledge as well as knowledge gaps related to conventional and genetically engineered probiotics. It also discusses the effect of GLP‐1 peptides in combination with bioengineered probiotics to prevent and treat diseases.
Doing the Right Thing? General Practitioners' Considerations in Achieving a Timely Dementia Diagnosis
Authors: Visser Fleur C. W. , van Eersel Marlise E. A. , van der Zaag‐Loonen Hester J. , Hempenius Liesbeth , Perry Marieke , van Munster Barbara C. 
Published: September 2024

ABSTRACTObjectivesTimely detection and diagnosis of dementia are beneficial for providing appropriate, anticipatory care and preventing acute situations. However, initiating diagnostic testing is a complex and dynamic process that requires general practitioners (GPs) to balance competing priorities. Previously identified barriers, such as a lack of time, knowledge, and resources, may not fully represent the challenges involved in this process. Therefore, this study aimed to examine GPs' more implicit considerations on starting the diagnostic trajectory for dementia.MethodsA qualitative study was conducted using semi‐structured interviews with 14 Dutch GPs who were purposively selected through maximum variation sampling. The interview transcripts were inductively analyzed in multiple rounds by a multidisciplinary research team using thematic analysis.ResultsGPs' considerations on starting the diagnostic trajectory for dementia can be summarized in three main themes that are interconnected: (1) ‘the presumed patient's willingness’, that is, facing a dilemma of wanting to respect patient autonomy in cases of denial or an absence of a diagnostic request, while at the same time identifying a problem and feeling the urgency to act; (2) ‘the GP's attempt not to harm’, that is, balancing between not wanting to harm the patient and/or relatives with the burdensome label of dementia and with the possible negative consequences of a late diagnosis; and (3) ‘time, trust, and interprofessional collaboration influence timeliness of diagnostic work‐up’, that is, time available for consultations, time as a diagnostic factor, GP's diagnostic confidence, and trustful physician–patient relationship.ConclusionsThis study revealed that important ethical dilemmas regarding patient autonomy and the principle of doing no harm lie behind practical GP barriers to initiating diagnostic testing for dementia. Time, trust, and interprofessional collaboration were found to facilitate GPs in determining the right decision and timing with each individual patient and their relatives. Future research could explore the value of diagnostic decision aids that explicitly involve patients and their relatives in this balancing act.
Suction‐Based Optical Coherence Elastography for the Biomechanical Characterization of Pathological Skin Conditions: A Pilot Study
Authors: van Haasterecht L. , Bartolini L. , Louter J. M. I. , González P. J. , Niessen F. B. , Iannuzzi D. , Groot M. L. , van Zuijlen P. P. M. 
Published: September 2024

ABSTRACTAccurate characterization of mechanical properties is crucial in the evaluation of therapeutic effects for problematic skin conditions. A pilot study was carried out using a novel optical coherence elastography (OCE) device, combining mechanical characterization through suction‐based deformation and imaging through optical coherence tomography. Using AI‐assisted image segmentation and a power‐law model, we were able to describe the mechanical behavior, comparing with measurements from the most commonly used commercial instrument (Cutometer) and subjective analyses of stiffness using the Patient and Observer Scar Assessment Scale. Twenty subjects were included with either keloids or hypertrophic scars. Measurements were fast and produced no discomfort. Mechanical and structural (epidermal thickness and rugosity) descriptors in pathologic skin conditions differed significantly from those in control tissue. We showed for the first time, the clinical feasibility of this novel suction‐based OCE device in evaluating mechanical and structural properties in pathological skin conditions such as scars.
45S5 Bioactive Glass‐Ointment Positively Effects on Wound Healing in Rats by Regulating TNFα, Il‐10, VEGF, and TGFβ
Authors: Kırgız Ömer , Altuğ Muhammed Enes , Özkan Hüseyin , Han Mehmet Cengiz , Akçakavak Gökhan , Özarslan Ali Can , Yücel Sevil 
Published: September 2024

ABSTRACTAimThis study aimed to investigate the effects of 45S5 bioactive glass‐ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.Materials and MethodsThirty‐two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21‐day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor‐alpha (TNFα), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor‐beta (TGFβ), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNFα, IL6, and IL10 proteins were measured by ELISA.ResultsIt was observed that the BG group showed anti‐inflammatory activity by suppressing TNFα levels and stimulating IL‐10. In addition, it was determined that BG increased fibroblast activity and vascularization.ConclusionCurrent findings showed that topical application of BG has anti‐inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing.
An incomplete picture: A scoping review of how scholars account for race and ethnicity in family homelessness research
Authors: Heaton Abigail 
Published: September 2024

AbstractFamilies of color in the United States experience homelessness at a disproportionately higher rate than White families; however, little is known about how scholars account for race and ethnicity in family homelessness research. This scoping review analyzes how researchers examine race and ethnicity in conceptual frameworks, methods, and analysis.Following PRISMA‐ScR reporting standards, I searched PubMed, PsycINFO, Scopus, and ERIC for quantitative studies including a housing outcome for homeless service‐involved families. I used Covidence to screen for inclusion and extract data, and QuADS to evaluate study quality.Fourteen studies met inclusion criteria. Researchers’ articles lacked theory and the context of racism, lacked detail on how race and ethnicity were conceptualized and operationalized, and most (71%) did not disaggregate results.Without putting data within the context of systemic racism and disaggregating outcomes, research will produce incomplete knowledge on family homelessness, leading to ineffective interventions for families of color.
Short‐form thymic stromal lymphopoietin (sfTSLP) restricts herpes simplex virus infection of human primary keratinocytes
Authors: Zeitvogel Jana , Döhner Katinka , Klug Ilona , Richardo Timmy , Sodeik Beate , Werfel Thomas 
Published: September 2024

AbstractEczema herpeticum (EH) is a disseminated severe herpes simplex virus type 1 (HSV‐1) infection that mainly occurs in a subset of patients suffering from atopic dermatitis (AD). EH is complex and multifaceted, involving immunological changes, environmental influences, and genetic aberrations. Certain genetic variants of the thymic stromal lymphopoietin (TSLP) may predispose to develop severe HSV‐1‐induced eczema. Therefore, we investigated the impact of TSLP on HSV‐1 infection. TSLP encodes for two distinct forms: a long‐form (lfTSLP), primarily associated with type 2 immunity, and a short‐form (sfTSLP) with anti‐inflammatory and antimicrobial properties. While sfTSLP reduced HSV‐1 infectibility in human primary keratinocytes (HPK), lfTSLP did not. In HPK treated with sfTSLP, HSV‐1 gene expression, and replication decreased, while virion binding to cells and targeting of incoming capsids to the nucleus were not diminished compared to untreated cells. sfTSLP caused only minor changes in the expression of innate immunity cytokines, and its inhibition of HSV‐1 infection did not require de novo protein synthesis. Time window experiments indicated a different antiviral mechanism than LL‐37. sfTSLP showed the strongest antiviral effect when administered to HPK before or after inoculation with HSV‐1, and outperformed the inhibitory potential of LL‐37 under these conditions. Our data show that sfTSLP has antiviral functions and promotes repression of the HSV‐1 infection in HPK.
Long lasting effect of intramuscular iodine injection in the treatment of goiter in an intestinal failure patient with complete entero‐colectomy
Authors: Belaid Sirine , Raghu Vikram , Alissa Feras , Rudolph Jeffrey 
Published: September 2024

AbstractIndividuals with intestinal failure are at risk of micronutrient deficiencies, including iodine, an essential trace element critical for thyroid hormone production. In patients entirely dependent on parenteral nutrition, options for replenishing and maintaining iodine levels are severely restricted as oral forms have limited absorption, and intravenous alternatives are unavailable. Ethiodized oil (Lipiodol‐TM) is an iodinated contrast agent with an unusually long half‐life that can be given orally or injected into a target organ. We report the successful use of intramuscular injection of ethiodized oil in treating goiter in a patient with total entero‐colectomy and in sustaining long lasting thyroid function for over 5 years.
Narrative reanalysis: A methodological framework for a new brand of reviews
Authors: Hall Steven , Leeder Erin 
Published: September 2024

AbstractIn response to the evolving needs of knowledge synthesis, this manuscript introduces the concept of narrative reanalysis, a method that refines data from initial reviews, such as systematic and reviews, to focus on specific sub‐phenomena. Unlike traditional narrative reviews, which lack the methodological rigor of systematic reviews and are broader in scope, our methodological framework for narrative reanalysis applies a structured, systematic framework to the interpretation of existing data. This approach enables a focused investigation of nuanced topics within a broader dataset, enhancing understanding and generating new insights. We detail a five‐stage methodological framework that guides the narrative reanalysis process: (1) retrieval of an initial review, (2) identification and justification of a sub‐phenomenon, (3) expanded search, selection, and extraction of data, (4) reanalyzing the sub‐phenomenon, and (5) writing the report. The proposed framework aims to standardize narrative reanalysis, advocating for its use in academic and research settings to foster more rigorous and insightful literature reviews. This approach bridges the methodological gap between narrative and systematic reviews, offering a valuable tool for researchers to explore detailed aspects of broader topics without the extensive resources required for systematic reviews.
Fast‐and‐frugal decision tree for the rapid critical appraisal of systematic reviews
Authors: Lorenz Robert C. , Jenny Mirjam , Jacobs Anja , Matthias Katja 
Published: September 2024

AbstractConducting high‐quality overviews of reviews (OoR) is time‐consuming. Because the quality of systematic reviews (SRs) varies, it is necessary to critically appraise SRs when conducting an OoR. A well‐established appraisal tool is A Measurement Tool to Assess Systematic Reviews (AMSTAR) 2, which takes about 15–32 min per application. To save time, we developed two fast‐and‐frugal decision trees (FFTs) for assessing the methodological quality of SR for OoR either during the full‐text screening stage (Screening FFT) or to the resulting pool of SRs (Rapid Appraisal FFT). To build a data set for developing the FFT, we identified published AMSTAR 2 appraisals. Overall confidence ratings of the AMSTAR 2 were used as a criterion and the 16 items as cues. One thousand five hundred and nineteen appraisals were obtained from 24 publications and divided into training and test data sets. The resulting Screening FFT consists of three items and correctly identifies all non‐critically low‐quality SRs (sensitivity of 100%), but has a positive predictive value of 59%. The three‐item Rapid Appraisal FFT correctly identifies 80% of the high‐quality SRs and correctly identifies 97% of the low‐quality SRs, resulting in an accuracy of 95%. The FFTs require about 10% of the 16 AMSTAR 2 items. The Screening FFT may be applied during full‐text screening to exclude SRs with critically low quality. The Rapid Appraisal FFT may be applied to the final SR pool to identify SR that might be of high methodological quality.
Development of a pre‐column derivatization ultra‐high‐performance liquid chromatography method for polysaccharide and monosaccharide quantification in Lycium barbarum
Authors: Zhao Zhilong , Bai Yunfeng , Chen Huan , Wang Weibiao , Ding Shuqin , Zhang Yue , Sa Yuping , Chen Guoning , Ma Xueqin 
Published: September 2024

Given the limited specificity and accuracy observed in the current official colorimetric quantification of polysaccharide in Lycium barbarum, our study aims to establish a novel, specific, accurate, and economic pre‐column derivatization ultra‐high‐performance liquid chromatography (UHPLC) method for determining the monosaccharide and polysaccharide content in L. barbarum. The optimization of extraction, hydrolysis, and derivatization (using 1‐phenyl‐3‐methyl‐5‐pyrazolone) processes for polysaccharide from L. barbarum was conducted initially, followed by separation of nine monosaccharides within 20 min using UHPLC with a C18 column. Subsequently, a novel method known as quantitative analysis of multiple components by single marker was developed, utilizing either additive 2‐deoxy‐D‐ribose or any monosaccharide present in the sample as a single reference standard to simultaneously detect the contents of polysaccharide and nine monosaccharides in L. barbarum. To validate the accuracy of the established method, the quantitative results of our approach were compared to both external and internal standard method methods. The minimal relative errors in the quantitative determination of monosaccharides among the three methods confirmed the dependability of the method. By analyzing 20 batches of L. barbarum samples, D‐galacturonic acid exhibited the highest content and the polysaccharide levels ranged from 3.02 to 13.04 mg/g. All data implied the specificity and accuracy of the method.
Enhancing memorable volunteer tourism experiences through mindfulness: A case of short‐term volunteer tourists
Authors: Eck Thomas , An Soyoung , Jiang Li , Choe Yunseon 
Published: September 2024

AbstractVoluntourism has been increasingly researched in tourism studies. However, the need remains to better understand what drives memorable experiences in such a context. This research applied mindfulness theory in the setting of voluntourism. The influence of mindfulness among voluntourists was studied along with dimensions of memorable tourism experiences (MTEs, i.e., hedonism, involvement, meaningfulness, knowledge, novelty, and local culture) and revisit intention among voluntourists. A structural model was employed with twelve hypotheses pertaining to data collected from 342 Chinese residents who had prior voluntourism experience before data collection in March 2020. For data analysis, confirmatory factor analysis and structural equation modeling were used to test relationships between variables. Data analysis demonstrated that mindfulness significantly influenced all six dimensions of MTEs in this study. Four of the dimensions of MTE (i.e., hedonism, involvement, meaningfulness, and local culture) significantly influenced revisit intention.
The bioinductive collagen implant yields positive histological, clinical and MRI outcomes in the management of rotator cuff tears: A systematic review
Authors: Longo Umile Giuseppe , Marino Martina , de Sire Alessandro , Ruiz‐Iban Miguel A. , D'Hooghe Pieter 
Published: September 2024

AbstractPurposeThe aim of this study is to report and discuss the outcomes of clinical, histological and animal studies exploring the application of bio‐inductive collagen implants (BCIs) to partial and full‐thickness rotator cuff tears (PT‐ and FT‐RCTs) in addition to reporting on cost‐related factors.MethodsReview of literature was performed using the PRISMA guidelines. A systematic electronic literature search was conducted using the CENTRAL, CINAHL, Cochrane Library, EBSCOhost, EMBASE and Google Scholar bibliographic databases. Microsoft Excel was used to create tables onto which extracted data were recorded. Tables were organized based on the research statement formulated using the PICO approach. No statistical analysis was performed.ResultsNine studies evaluated clinical and MRI outcomes of BCI augmentation for FT‐RCTs, seven evaluated similar outcomes when applied to PT‐RCTs, two additional studies were case reports and three studies assessed application to FT‐ and PT‐RCTs without stratification of results, one of which also reported on histological data. Two studies reported on histological data alone, and finally, two reported on healthcare costs. BCI augmentation, alone and combined with rotator cuff repair (RCR), displays generally good histological, postoperative clinical and MRI outcomes for PT‐ and FT‐RCT treatment. Recent economic analyses seem to be in favour of the use of this procedure, when selected and applied for appropriate patient populations.ConclusionSeveral studies have shown promising results of BCI application to PT‐ and FT‐RCTs, both concomitantly and independently from RCR. Investigations report promising histological characteristics, improved clinical outcomes, increased tendon thickness, reduced defect size and lower re‐tear rates.Level of EvidenceLevel IV.
A Comprehensive Study on Young Roots of Acacia mangium Willd. Species for Soil Bioengineering
Authors: Phan Trung Nghia , Kraithong Pinyapat , Likitlersuang Chanud , Likitlersuang Suched 
Published: September 2024

ABSTRACTSoil bioengineering has been leveraged for controlling shallow landslides and soil erosion. Acacia mangium Willd. species is widely used for soil bioengineering, but its root properties have not been extensively investigated. This study aimed to measure the various properties (i.e., morphological traits, biomechanical properties, chemical composition and root reinforcement) for establishing roots of A. mangium Willd. species through a series of laboratory experiments (i.e., rhizobox observation, uniaxial tensile test, direct shear and volumetric water content and suction monitoring). The results underscored the rapid growth (i.e., 0.43 ± 0.01 cm/day) and highlighted variations in “side” root area ratios of A. mangium Willd. species with depth. Root orientation also demonstrated a significant correlation with root diameter following a linear model (p < 0.05, R
2 = 0.39). At the early‐stage establishment (28 weeks), A. mangium Willd. roots exhibited the tensile strength, Young's modulus and mechanical reinforcement of 10.18 ± 0.62 MPa, 325.18 ± 21.72 MPa and 3.15 kPa, respectively. The presence of roots was found to increase the soil suction, air‐entry value and saturated water content of lateritic soil by improving the soil‐water characteristic curve. This work elucidated the root properties of A. mangium Willd. during the early‐stage establishment, raising caution for the design of slope‐stabilisation projects using soil bioengineering, especially during the initial phase of projects.
Circular RNAs in human diseases
Authors: Wang Yuanyong , Zhang Jin , Yang Yuchen , Liu Zhuofeng , Sun Sijia , Li Rui , Zhu Hui , Li Tian , Zheng Jin , Li Jie , Ma Litian 
Published: September 2024

AbstractCircular RNAs (circRNAs) are a unique class of RNA molecules formed through back‐splicing rather than linear splicing. As an emerging field in molecular biology, circRNAs have garnered significant attention due to their distinct structure and potential functional implications. A comprehensive understanding of circRNAs’ functions and potential clinical applications remains elusive despite accumulating evidence of their involvement in disease pathogenesis. Recent research highlights their significant roles in various human diseases, but comprehensive reviews on their functions and applications remain scarce. This review provides an in‐depth examination of circRNAs, focusing first on their involvement in non‐neoplastic diseases such as respiratory, endocrine, metabolic, musculoskeletal, cardiovascular, and renal disorders. We then explore their roles in tumors, with particular emphasis on exosomal circular RNAs, which are crucial for cancer initiation, progression, and resistance to treatment. By detailing their biogenesis, functions, and impact on disease mechanisms, this review underscores the potential of circRNAs as diagnostic biomarkers and therapeutic targets. The review not only enhances our understanding of circRNAs’ roles in specific diseases and tumor types but also highlights their potential as novel diagnostic and therapeutic tools, thereby paving the way for future clinical investigations and potential therapeutic interventions.
Frameshift Mutation in PAX2 Related to Focal Segmental Glomerular Sclerosis: A Case Report and Literature Review
Authors: Hu Xueling , Lin Wei , Luo Zengyuan , Zhong Yong , Xiao Xiangcheng , Tang Rong 
Published: September 2024

ABSTRACTBackgroundPaired box gene 2 (PAX2) heterozygous mutations can cause renal coloboma syndrome, but its role in patients with focal segmental glomerular sclerosis (FSGS) has been rarely reported.MethodsBased on the clinical manifestations and renal pathological characteristics of the patient, as well as familial whole exome sequencing, the diagnosis of FSGS related to PAX2 mutation was confirmed. Treatment such as lowering urinary protein and blood pressure was given, and the patient was followed up and observed.ResultsThere is a familial heterozygous case presented with chronic kidney disease secondary to FSGS, which was related to PAX2 frameshift mutation due to the deletion of G at the position 76 (c.76delG). To our knowledge, this is the first report of PAX2 c.76delG variant related to adult‐onset FSGS.ConclusionHere, we further expand the phenotypic spectrum of FSGS. Genetic screening especially PAX2 mutation is recommended in patients with adult‐onset FSGS of unknown etiology.