bluuebunny/crossref_metadata_2025_split huggingface dataset:
The T4-L1-Hip Axis: Sagittal Spinal Realignment Targets in Long-Construct Adult Spinal Deformity Surgery
Authors: Hills Jeffrey , Mundis Gregory M. , Klineberg Eric O. , Smith Justin S. , Line Breton , Gum Jeffrey L. , Protopsaltis Themistocles S. , Hamilton D. Kojo , Soroceanu Alex , Eastlack Robert , Nunley Pierce , Kebaish Khaled M. , Lenke Lawrence G. , Hostin Richard A. , Gupta Munish C. , Kim Han Jo , Ames Christopher P. , Burton Douglas C. , Shaffrey Christopher I. , Schwab Frank J. , Lafage Virginie , Lafage Renaud , Bess Shay , Kelly Michael P. ,   on behalf of the International Spine Study Group
Published: September 2024

Background:
Our understanding of the relationship between sagittal alignment and mechanical complications is evolving. In normal spines, the L1-pelvic angle (L1PA) accounts for the magnitude and distribution of lordosis and is strongly associated with pelvic incidence (PI), and the T4-pelvic angle (T4PA) is within 4° of the L1PA. We aimed to examine the clinical implications of realignment to a normal L1PA and T4-L1PA mismatch.

Methods:
A prospective multicenter adult spinal deformity registry was queried for patients who underwent fixation from the T1-T5 region to the sacrum and had 2-year radiographic follow-up. Normal sagittal alignment was defined as previously described for normal spines: L1PA = PI × 0.5 − 21°, and T4-L1PA mismatch = 0°. Mechanical failure was defined as severe proximal junctional kyphosis (PJK), displaced rod fracture, or reoperation for junctional failure, pseudarthrosis, or rod fracture within 2 years. Multivariable nonlinear logistic regression was used to define target ranges for L1PA and T4-L1PA mismatch that minimized the risk of mechanical failure. The relationship between changes in T4PA and changes in global sagittal alignment according to the C2-pelvic angle (C2PA) was determined using linear regression. Lastly, multivariable regression was used to assess associations between initial postoperative C2PA and patient-reported outcomes at 1 year, adjusting for preoperative scores and age.

Results:
The median age of the 247 included patients was 64 years (interquartile range, 57 to 69 years), and 202 (82%) were female. Deviation from a normal L1PA or T4-L1PA mismatch in either direction was associated with a significantly higher risk of mechanical failure, independent of age. Risk was minimized with an L1PA of PI × 0.5 − (19° ± 2°) and T4-L1PA mismatch between −3° and +1°. Changes in T4PA and in C2PA at the time of final follow-up were strongly associated (r2 = 0.96). Higher postoperative C2PA was independently associated with more disability, more pain, and worse self-image at 1 year.

Conclusions:
We defined sagittal alignment targets using L1PA (relative to PI) and the T4-L1PA mismatch, which are both directly modifiable during surgery. In patients undergoing long fusion to the sacrum, realignment based on these targets may lead to fewer mechanical failures.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Liposomal Bupivacaine Does Not Decrease Postoperative Pain in Patients with Intracapsular Femoral Neck Fracture Treated with Hemiarthroplasty
Authors: Kang Kevin K. , Voyvodic Lucas , Komlos Daniel , Swiggett Samuel , Ng Mitchell K. 
Published: September 2024

Background:
Liposomal bupivacaine (LB) is a long-lasting local anesthetic agent that was developed for use in the surgical setting to help manage postoperative pain. The objective of this study was to evaluate the effect of LB on postoperative pain, function, and overall hospital course in patients with intracapsular hip fractures who were treated with hip hemiarthroplasty.

Methods:
This was a single-center, randomized prospective double-blinded study of 50 patients with an isolated intracapsular femoral neck fracture who were treated with hip hemiarthroplasty from 2018 to 2022. The study group consisted of 25 patients who were treated with intraoperative LB and bupivacaine hydrochloride injections, while the control group consisted of 25 patients who were treated with intraoperative bupivacaine hydrochloride injections only. Primary outcomes were a visual analog scale (VAS) score for pain, total morphine milligram equivalents (MME), delirium, and time to ambulation with physical therapy.

Results:
No significant differences between the study and control groups were found in any of the outcomes measured. Most notably, there were no differences in the average postoperative pain score (VAS, 2.26 versus 2.7; p = 0.34), total MME used postoperatively (11.73 versus 9.98 MME; p = 0.71), and postoperative day of discharge (4.00 versus 3.88 days; p = 0.82).

Conclusions:
The results of our study suggest that use of LB is not associated with substantially improved postoperative pain or function or with a shorter hospital course following hip hemiarthroplasty for a femoral neck fracture. Given the higher cost of LB compared with standard postoperative pain modalities, it is worth questioning its use in the setting of geriatric hip fractures.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
High patient acceptance of immediately sequential bilateral cataract surgery (ISBCS) as part of a one-stop see-and-treat pathway within an innovative NHS cataract unit
Authors: Roberts Harry , Alsusa Maher , Ahmad Shakeel , Smith Zoe , Evans Sam , Wilkinson Elizabeth 
Published: September 2024

Abstract

Background
Constituting ~0.5% of all NHS cataract operations, national provision of immediately sequential bilateral cataract surgery (ISBCS) is limited. Combining offering ISBCS within a novel one-stop see-and-treat (S&T) cataract pathway would offer patients the opportunity for two cataract operations in a single hospital visit. Patient acceptance of ISBCS amongst urban populations has been investigated. However, little is understood about ISBCS acceptance rurally.
Methods
Retrospective observational study at the Nightingale Hospital, Exeter investigating patient acceptance of ISBCS within S&T; following the implementation of a S&T cataract pathway entailing a pre-operative patient-clinician telephone consultation and subsequently scheduled single date of assessment and surgery. Patient acceptance and factors potentially influencing decisions were investigated.
Results
200 patient telephone consultations between 22nd August 2023 and 9th January 2024 were evaluated. 198 (99%) patients referred were suitable for S&T cataract surgery, of whom 109 (54.5%) were deemed eligible for offering ISBCS S&T cataract surgery. Of the eligible participants, 78 (71.56%) favoured ISBCS. No significant differences in age, sex, distance from hospital or refractive data were identified between ISBCS accepting and declining participants.
Conclusions
Our results illustrate a high patient acceptance rate (71.56%) of ISBCS within our population in contrast with published national rates. Offering ISBCS within a S&T model would allow patients to benefit from having both cataracts assessed and treated within a single hospital visit.
WITHDRAWN: Amelioration of retinal injury and improvement in associated memory by hUCB-derived cells is dose-dependent
Authors: Mehra Priya , Rain Manjari , SINGH JAGTAR , Saha Pradip Kumar , ANAND AKSHAY 
Published: September 2024

Abstract

Background- The visual information transfers from retina to brain to amplify the neuronal signals resulting in visual perception. Damage in the retinal layer causes visual field defects such as cognition decline and memory loss. Although, various research investigations have attempted to reverse the damage using therapeutic interventions, however, these have not been successfully translated. In this study we aim to evaluate the efficacy of lineage negative stem cells derived from hUCB in reversing the retinal injury and visual memory by subretinal transplantation in mice model of laser injury.
Methods- Retinal injury was introduced in C57BL/6J male mice (24-28g) by using laser photocoagulation around the optic disc with defined parameters that disrupted the RPE layer of the retina. The 2 laser spots (2L) and 8 laser spots (8L) were created in each eye of the mice. The Fundus Fluorescein Angiography was used to confirm the establishment of varying degree of retinal injury. Around 50,000 stem cells were transplanted in each eye after 24 hours of laser injury. After 1 month, neurobehavioral assessments were carried out to estimate the visual-spatial memory using Morris Water Maze (MWM) and Passive Avoidance. Subsequently, the molecular assays including RT-PCR and immunohistochemistry were also undertaken.
Results- Fundus angiography confirmed presence of retinal vein leakage in the injury model in comparison to healthy control. To identify the cognitive deficits, the neurobehavioral assessments, based on Morris water maze and passive avoidance, were performed. The neurobehavioral tests show learning and cognitive improvement in the stem cell group in comparison to the injury group. Further, gene expression of neurotrophic factors, proliferative and apoptotic factors showed upregulated neuronal activity and possible neuroprotective role in rescue of retinal injury in stem cell group.
Conclusion-The Lin –ve stem cells rescued the injury and reversed the visual memory and retinal injury. The study shows that, with degree of injury, the number of lin –ve stem cells should be increased.
Unveiling the conversion mechanism of glioblastoma from the mesenchymal to the proneural subtype driven by HDAC1/p-SMAD3-TP53I11 axis
Authors: Zhao Kai , Zhao Hai , Shang Rui , Liang Hua , Wang Chao , Zhu Meng , Chen Wujun 
Published: September 2024

Abstract

Mesenchymal glioblastoma (MES GBM) is characterized by rapid proliferation, extensive invasion, and formidable treatment resistance. Addressing these characteristics in MES cells is crucial for improving patient prognosis. Here, we discovered the MES GBM subtype conversion mechanism driven by HDAC1/p-SMAD3-TP53I11 in this study. First, the impact of HDAC1 inhibitors and bevacizumab on the phenotypic characteristics of MES cells was assessed. Co-immunoprecipitation (Co-IP) and immunofluorescence techniques elucidated the epigenetic mechanism of HDAC1. Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA-seq identified downstream transcribed genes. We found Inhibition or knockdown of HDAC1 transformed MES characteristics into proneural (PN) characteristics, prolonged survival in patient-derived xenograft (PDX) models, and suppressed in vitro cell proliferation and invasion. RG2833 (an HDAC1 inhibitor) was found to enhance histone acetylation, promoting the binding of the transcription factor p-SMAD3 to the genome. Immunoprecipitation experiments revealed an interaction between p-SMAD3 and HDAC1. RNA-seq and ChIP-seq data analysis from MES cell lines before and after RG2833 treatment identified Tumor Protein P53 Inducible Protein 11 (TP53I11) as a downstream gene. The study indicates that by intervening HDAC1/p-SMAD3-TP53I11, HDAC1 can serve as a promising therapeutic target for the treatment of MES GBM.
Suitable ecological niches of invasive malaria vector under present and projected climatic conditions in South of Iran
Authors: Abbasi Madineh , Yaghoobi-Ershadi Mohammad Reza , Hanafi-Bojd Ahmad Ali 
Published: September 2024

Abstract

Background Unfortunately, the resurgence of malaria occurred in Iran after three years of free malaria conditions, from 2022. Efforts to control malaria through surveillance, diagnosis, treatment and prevention measures have shown progress, but climate change may pose challenges to these efforts, potentially increasing the epidemic potential of malaria in susceptible regions. The research look for to predict the current and future geographical range and suitability ofAnopheles stephensimosquitoes in southern Iran. This information is important for assessing the risk of disease transmission and developing successful strategies for controlling these vectors in the future. Method The study compiled a database ofAn. stephensifindings in Hormozgan province based on field studies and utilized various scientific databases to gather relevant data. Geographical coordinates and distribution data of the species were employed for mapping and forecasting its spread under current and future climate conditions. A total of 19 bioclimatic variables were used for ecological niche prediction by the Maximum Entropy Model. The MaxEnt software was employed to evaluate potential changes in the spatial distribution ofAn. stephensiin the future, with the model's performance assessed using ROC analysis and AUC values. ResultsAnopheles stephensidistribution in Hormozgan province was studied over the past three decades, with 101 locations reported. The MaxEnt model predicts changes in distribution under different climate scenarios. The model's strong performance was demonstrated by ROC analysis, with AUC values ranging from 0.81 to 0.85 for training data and 0.62 to 0.72 for test data. Five key bioclimatic variables were identified, with Isothermality being the most impactful. The study highlights the significant influence of the Mean Temperature of the Driest Quarter. The modeling outcomes indicate that roughly 19–27% of the province's territory has a significant likelihood of An. stephensi thriving and expanding. Discussion The model suggests that 19–27% of the province's land is highly conducive toAn. stephensi, with concentrated areas of suitability in the western part of Minab County. The study emphasizes the importance of taking proactive steps to tackle the effects of climate change on diseases carried by vectors, such as malaria.
TERT Promoter Mutations Combined with TP53 Mutations as Potential Biomarkers for Hepatocellular Carcinoma Recurrence and Prognosis
Authors: Li Jin , Bai Ling , Xin Zhaodan , Song Jiajia , Chen Hao , Song Xingbo , Zhou Juan 
Published: September 2024

Abstract

Hepatocellular carcinoma (HCC) is the most prevalent form of liver cancer, and ranks among the most lethal malignancies globally, primarily due to its high rates of recurrence and metastasis. Despite the urgency, no reliable biomarkers currently exist for predicting tumor recurrence in HCC. Telomerase reverse transcriptase (TERT) promoter mutations (TERTpm) and cellular tumor antigen p53 mutations (TP53m) have been frequently documented in HCC, but their combined clinical significance remains undefined. In this study, we investigated the clinical implications of TERTpm, TP53m, and their co-occurrence in 50 HCC tissue samples using the next-generation sequencing (NGS) technology. We identified TERTpm (C228T) and TP53m in 16 (32%) and 24 (48%) samples, respectively. Our findings indicate that these mutations are more prevalent in male patients (100% for TERTpm, 83.33% for TP53m), in those with solitary tumors (87.5% for both), in individuals with G2-G3 hepatitis (100% / 83.3%), and in cases of moderately differentiated tumors (75.0% / 83.3%). Furthermore, patients with both TERTpm and TP53m exhibited a significantly higher risk of tumor relapse (P < 0.05) and shorter progression-free survival (P < 0.05). Collectively, our results suggest that presence of both TERTpm and TP53m may serve as a robust predictor of tumor recurrence and a marker of poor prognosis in HCC.
Metabolic syndrome modulates risk of endometrial cancer regardless of menopause status- A UK BIOBANK Study
Authors: Karkia Rebecca , Maccarthy Gideon , Payne Annette , Karteris Emmanouil , Pazoki Raha , Chatterjee Jayanta , Karkia Rebecca 
Published: September 2024

Abstract

Background
This study investigates the association between endometrial cancer (EC) risk and features of metabolic syndrome (MetS) using the UK Biobank.
Methods
Univariate and multivariate analysis of EC risk and features of MetS including serum biochemistry were analysed. Subgroup analysis was also undertaken for pre- and post-menopausal participants.
Results
203,644 females from the UK Biobank were included in this study. 49,071 (43.8%) met the met the International Diabetes Federation (IDF) definition of MetS and in these females the risk of EC was almost threefold higher (OR = 2.67; 95%CI:2.41–2.96, P < 0.0001). Of those participants who developed EC (n = 1632), Waist circumference > 80cm, BMI > 30kg/m2, hypertension > 130/80mmHg and hyperlipidaemia or diabetes were significantly associated with increased risk of EC. BMI > 30 kg/m2 alone was associated with threefold higher risk and BMI > 40 kg/m2 a ninefold higher risk. Associations remained significant in pre and postmenopausal subgroups. Treatment for hypertension, hyperlipidaemia or diabetes was associated with EC risk in univariate analysis but did not remain significant in multivariate analysis. Having abnormal lipid profile, fasting hyperglycaemia or hypertension significantly increased the risk of EC after correction for confounding factors.
Conclusion
Features of MetS, both independently and in combination, significantly increase the risk of EC. Screening those with obesity for MetS, in pre-menopausal years may help to identify those at highest risk.
A Comparative Study of transurethral 450 nm DiLVP and 1470 nm DiLEP in the treatment of Benign Prostatic Hyperplasia
Authors: Wang Zhichao , Xing Qingfei , Huang Zhongxian , Gao Xinghua , Qiu Mengzhen , Zhang Longyang 
Published: September 2024

Abstract

Objective
To compare the clinical effects of 450 nm diode laser vaporisation of the prostate (450 nm DiLVP) and 1470 nm diode laser enucleation of the prostate (1470 nm DiLEP) in the surgical treatment of benign prostate hyperplasia (BPH).
Methods
BPH patients who were treated with 450 nm DiLVP or 1470 nm DiLEP in the Department of Urology of Jinan Central Hospital from November in 2021 to November in 2023 were selected for retrospective analysis. The assessment indexes included: (1) Preoperative general information; (2) Perioperative indicators; (3) Postoperative follow-up indicators(followed up at 1 and 3 months after surgery).
Results
A total of 128 BPH patients were included in the study, 63 in the experimental group with 450 nm DiLVP and 65 in the control group with 1470 nm DiLEP. There was no significant difference in the baseline information between the two groups (P > 0.05). Compared with the 1470 nm DiLEP group, the operation time, the laser energy consumption and bladder irrigation time were significantly reduced in the 450 nm DiLVP group (P < 0.001). At 3 months postoperatively, Qmax relief was significant in the 1470 nm DiLEP group (P = 0.023).
Conclusions
(1)Perioperatively, compared with the 1470 nm DiLEP, 450 nm DiLVP has shorter operation time, irrigation time, and laser energy consumption; (2)Postoperative follow-up: 450 nm DiLVP and 1470 nm DiLEP have similar efficacy in improving BPH symptoms; 1470 nm DiLEP may have greater impact on max urinary flow rate in long-tem follow-up, which needs further verification.
Research on Intervention Strategies and Neural Mechanisms for Competence Frustration: A Case of Fire Rescue Personnel
Authors: WANG Yarong , ZHANG Runyu , Liu Ying , Jie REN 
Published: September 2024

Abstract

Background
With the rapid development of knowledge economy society and the sudden increase of social pressure, people in all walks of life generally suffer competency need frustration. Fire rescue personnel as a typical high-load, high-pressure, high-risk occupation, are easily suffering competence need frustrated. Therefore, this study chooses fire rescue personnel as the research object, aims to accurately measure the level of competence frustration and what measures to take to alleviate the negative effects of competence frustration.
(2) Methods: 20 graduate students were selected to verify the effect of frontal lobe α asymmetry (FAA) as a judgement of the competence frustration level using an EEG experimental method. On this basis, through two stopwatch stopping experiments, 72 fire rescue personnel were selected to conduct the experiments to investigate the intervention effects of developmental feedback and compassion-focused therapy.
(3) Results: Frontal α asymmetry could be used as an EEG indicator for judging competence frustration, and the compassion-focused therapy intervention reduced the level of competence frustration of the participants, while the developmental feedback intervention improved the level of competence frustration of the participants.
(4) Conclusions: The difference in the effects of the two intervention methods indicates that when intervening in competence frustration, it is easier to reduce competence frustration by focusing on the subjects themselves than by focusing on the completion of the task.
Preventive effect of PC-SOD on oxaliplatin-induced peripheral neuropathy in rats
Authors: Qiao Zhiwei , Akimoto Shouta , Kaji Noriko , Fukuda Koichiro , Mizushima Tohru 
Published: September 2024

Abstract

Oxaliplatin is widely used as a chemotherapeutic agent for treating various cancers, including colorectal cancer. However, oxaliplatin-induced peripheral neuropathy diminishes the quality of life of patients and leads to a reduction in the chemotherapy dose or even early discontinuation. Thus, the development of preventive or therapeutic drugs for oxaliplatin-induced peripheral neuropathy is urgently needed and may enhance its therapeutic effect. Herein, we aimed to investigate whether PC-SOD, a derivative of SOD with higher stability in the plasma and higher affinity for tissues, could prevent oxaliplatin-induced peripheral neuropathy. A neurite outgrowth assay using cultured PC12 cells and a rat model of oxaliplatin-induced peripheral neuropathy were used to elucidate the neuroprotective effects of PC-SOD. PC-SOD effectively attenuated oxaliplatin-induced neurite damage in cultured PC12 cells. In a rat model, PC-SOD reduced oxaliplatin-induced mechanical allodynia, cold hyperalgesia, and morphological damage to intraepidermal nerve fibres and dorsal root ganglion. Furthermore, PC-SOD did not affect the anti-tumour effects of oxaliplatin in cultured tumour cells or in tumour cell-implanted mice. These findings indicated that PC-SOD alleviated oxaliplatin-induced mechanical allodynia, cold hyperalgesia, and neural damage without affecting the antitumor activity of oxaliplatin. Therefore, PC-SOD is a promising drug candidate for the prevention of oxaliplatin-induced peripheral neuropathy.