bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of ferroptosis-triggered pyroptotic signaling in heart failure
Authors: Bi Xukun , Wu Xiaotian , Chen Jiaqi , Li Xiaoting , Lin Yangjun , Yu Yingying , Fang Xuexian , Cheng Xihao , Cai Zhaoxian , Jin Tingting , Han Shuxian , Wang Meihui , Han Peidong , Min Junxia , Fu Guosheng , Wang Fudi 
Published: September 2024

AbstractPressure overload–induced cardiac hypertrophy is a common cause of heart failure (HF), and emerging evidence suggests that excessive oxidized lipids have a detrimental effect on cardiomyocytes. However, the key regulator of lipid toxicity in cardiomyocytes during this pathological process remains unknown. Here, we used lipidomics profiling and RNA-seq analysis and found that phosphatidylethanolamines (PEs) and Acsl4 expression are significantly increased in mice with transverse aortic constriction (TAC)–induced HF compared to sham-operated mice. In addition, we found that overexpressing Acsl4 in cardiomyocytes exacerbates pressure overload‒induced cardiac dysfunction via ferroptosis. Notably, both pharmacological inhibition and genetic deletion of Acsl4 significantly reduced left ventricular chamber size and improved cardiac function in mice with TAC-induced HF. Moreover, silencing Acsl4 expression in cultured neonatal rat ventricular myocytes was sufficient to inhibit hypertrophic stimulus‒induced cell growth. Mechanistically, we found that Acsl4-dependent ferroptosis activates the pyroptotic signaling pathway, which leads to increased production of the proinflammatory cytokine IL-1β, and neutralizing IL-1β improved cardiac function in Acsl4 transgenic mice following TAC. These results indicate that ACSL4 plays an essential role in the heart during pressure overload‒induced cardiac remodeling via ferroptosis-induced pyroptotic signaling. Together, these findings provide compelling evidence that targeting the ACSL4-ferroptosis-pyroptotic signaling cascade may provide a promising therapeutic strategy for preventing heart failure.
Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system
Authors: Chang Yanli , Zhu Jiangli , Li Xiaopeng , Deng Yi , Lai Birou , Ma Yidan , Tong Jia , Liu Huicong , Li Juanjuan , Yang Chenyu , Chen Qiao , Lu Chengbiao , Liang Yinming , Qi Shiqian , Wang Xiaoning , Kong Eryan 
Published: September 2024

AbstractThe downregulation of Cadm4 (Cell adhesion molecular 4) is a prominent feature in demyelination diseases, yet, the underlying molecular mechanism remains elusive. Here, we reveal that Cadm4 undergoes specific palmitoylation at cysteine-347 (C347), which is crucial for its stable localization on the plasma membrane (PM). Mutation of C347 to alanine (C347A), blocking palmitoylation, causes Cadm4 internalization from the PM and subsequent degradation. In vivo experiments introducing the C347A mutation (Cadm4-KI) lead to severe myelin abnormalities in the central nervous system (CNS), characterized by loss, demyelination, and hypermyelination. We further identify ZDHHC3 (Zinc finger DHHC-type palmitoyltransferase 3) as the enzyme responsible for catalyzing Cadm4 palmitoylation. Depletion of ZDHHC3 reduces Cadm4 palmitoylation and diminishes its PM localization. Remarkably, genetic deletion of ZDHHC3 results in decreased Cadm4 palmitoylation and defects in CNS myelination, phenocopying the Cadm4-KI mouse model. Consequently, altered Cadm4 palmitoylation impairs neuronal transmission and cognitive behaviors in both Cadm4-KI and ZDHHC3 knockout mice. Importantly, attenuated ZDHHC3-Cadm4 signaling significantly influences neuroinflammation in diverse demyelination diseases. Mechanistically, we demonstrate the predominant expression of Cadm4 in the oligodendrocyte lineage and its potential role in modulating cell differentiation via the WNT-β-Catenin pathway. Together, our findings propose that dysregulated ZDHHC3-Cadm4 signaling contributes to myelin abnormalities, suggesting a common pathological mechanism underlying demyelination diseases associated with neuroinflammation.
The role(s) of NF-Y in development and differentiation
Authors: Dolfini Diletta , Imbriano Carol , Mantovani Roberto 
Published: September 2024

Abstract
NF-Y is a conserved sequence-specific trimeric Transcription Factor -TF- binding to the CCAAT element. We review here the role(s) in development, from pre-implantation embryo to terminally differentiated tissues, by rationalizing and commenting on genetic, genomic, epigenetic and biochemical studies. This effort brings to light the impact of NF-YA isoforms on stemness and differentiation, as well as binding to distal vs promoter proximal sites and connections with selected TFs.
Mental illness and COVID-19 vaccination: a multinational investigation of observational & register-based data
Authors: Barker Mary M. , Kõiv Kadri , Magnúsdóttir Ingibjörg , Milbourn Hannah , Wang Bin , Du Xinkai , Murphy Gillian , Herweijer Eva , Gísladóttir Elísabet U. , Li Huiqi , Lovik Anikó , Kähler Anna K. , Campbell Archie , Feychting Maria , Hauksdóttir Arna , Joyce Emily E. , Thordardottir Edda Bjork , Frans Emma M. , Hoffart Asle , Mägi Reedik , Tómasson Gunnar , Ásbjörnsdóttir Kristjana , Jakobsdóttir Jóhanna , Andreassen Ole A. , Sullivan Patrick F. , Johnson Sverre Urnes , Aspelund Thor , Brandlistuen Ragnhild Eek , Ask Helga , McCartney Daniel L. , Ebrahimi Omid V. , Lehto Kelli , Valdimarsdóttir Unnur A. , Nyberg Fredrik , Fang Fang 
Published: September 2024

AbstractIndividuals with mental illness are at higher risk of severe COVID-19 outcomes. However, previous studies on the uptake of COVID-19 vaccination in this population have reported conflicting results. Using data from seven cohort studies (N = 325,298) included in the multinational COVIDMENT consortium, and the Swedish registers (N = 8,080,234), this study investigates the association between mental illness (defined using self-report measures, clinical diagnosis and prescription data) and COVID-19 vaccination uptake. Results from the COVIDMENT cohort studies were pooled using meta-analyses, the majority of which showed no significant association between mental illness and vaccination uptake. In the Swedish register study population, we observed a very small reduction in the uptake of both the first and second dose of a COVID-19 vaccine among individuals with vs. without mental illness; the reduction was however greater among those not using psychiatric medication. Here we show that uptake of the COVID-19 vaccine is generally high among individuals both with and without mental illness, however the lower levels of vaccination uptake observed among subgroups of individuals with unmedicated mental illness warrants further attention.
Reconciling remote-sensing estimates of Ryugu’s albedo with laboratory measurements
Authors: Potiszil Christian , Ota Tsutomu , Nakamura Eizo 
Published: September 2024

AbstractThe Japanese Hayabusa2 spacecraft returned samples from the asteroid Ryugu in December 2020, and several results on the properties of the particles have been published since. A detailed geochemical analysis determined that the total organic carbon abundance of the sample was on average 2.92 wt%. This value is much less than the 14.6–59.3 vol% estimated from remote-sensing data for Ryugu coupled with the albedos of irradiated meteorites and organic matter. Understanding the reasons for this discrepancy is paramount to accurately predict the composition of C-complex asteroids. Here we explore several factors that may affect the estimates of organic matter abundance made using remote-sensing data. Such factors include the distribution of organic matter, the porosity and roughness of the surfaces of asteroids, and inconsistencies between the observation geometries used by remote-sensing and laboratory investigations. We posit that discussing such factors may fuel further experimental investigations of the effects of irradiation on asteroid surfaces and, thus, improve predictions of their organic composition.
Adherence to long-term telemonitoring-supported physical activity in patients with chronic heart failure
Authors: Lödding Pauline , Beyer Sebastian , Pökel Christoph , Kück Momme , Leps Christian , Radziwolek Lukas , Kerling Arno , Haufe Sven , Schulze Antina , Kwast Stefan , Voß Johannes , Kubaile Christian , Tegtbur Uwe , Busse Martin 
Published: September 2024

AbstractChronic heart failure (CHF) is one of the most common diseases with a prevalence of 1–2% in adults, disproportionately affecting the elderly. Despite consistent drug therapy, physical activity (PA) is an integral part of current guidelines. Yet adherence to regular PA and exercise interventions is poor and potential predictors and barriers to PA remain elusive. We examined the effects of a telemonitoring-based exercise intervention in 699 CHF patients in a prospective, randomized-controlled (1:1), multicenter trial. The study was registered in the German Clinical Trials Register under DRKS00019022 on 28.05.2020. For both, the exercise and control group, self-reported PA (MET*h/week) increased and sedentary behavior declined during the 12-month intervention period. In the exercise group, daily step count as analyzed via activity trackers remained stable (pre: 6459 [4016] steps/day, post: 6532 [3858] steps/day; p = 0.621). The average number of completed exercise instruction videos provided via an online application was 1.50 [1.44] videos/week at the beginning and gradually decreased to 1.00 [1.50] videos/week; p < 0.001). Multivariate regression model revealed that exercise-related PA (MET*h/week) and exercise capacity (Wmax) at baseline, CHF severity, atrial fibrillation and age predicted changes in self-reported exercise-related PA (R2 = 0.396). Furthermore, the BMI and the average number of completed videos per week at baseline were associated with the change in completed videos over the course of the study (R2 = 0.251). Our results show the influence of certain baseline characteristics as barriers and predictors of PA progression. Therefore, exercise programs should pay attention to patients’ individual conditions to set achievable goals, and eventually affect the adherence and sustainability of exercise-focused interventions.
Development and Evaluation of a Rural Longitudinal Neonatal Resuscitation Program Telesimulation Program (MOOSE: Maine Ongoing Outreach Simulation Education)
Authors: Melendi Misty , Zanno Allison E. , Holmes Jeffrey A. , Chipman Micheline , Cutler Anya , Stoddard Henry , Seften Leah M. , Gilbert Anna , Ottolini Mary , Craig Alexa , Mallory Leah A. 
Published: September 2024

Objective Neonatal resuscitation is a high-acuity, low-occurrence event and many rural pediatricians report feeling underprepared for these events. We piloted a longitudinal telesimulation (TS) program with a rural hospital's interprofessional delivery room teams aimed at improving adherence to Neonatal Resuscitation Program (NRP) guidelines and teamwork.
Study Design A TS study was conducted monthly in one rural hospital over a 10-month period from November 2020 to August 2021. TS sessions were remotely viewed and debriefed by experts, a neonatologist and a simulation educator. Sessions were video recorded and assessed using a scoring tool with validity evidence for NRP adherence. Teamwork was assessed using both TeamSTEPPS 2.0 Team Performance Observation Tool and Mayo High-Performance Teamwork Scale.
Results We conducted 10 TS sessions in one rural hospital. There were 24 total participants, who rotated through monthly sessions, ensuring interdisciplinary team composition was reflective of realistic staffing. NRP adherence rate for full code scenarios improved from a baseline of 39 to 95%. Compared with baseline data for efficiency, multiple NRP skills improved (e.g., cardiac lead placement occurred 12× faster, 0:31 seconds vs. 6:21 minutes). Teamwork scores showed improvement in all domains.
Conclusion Our results demonstrate that a TS program aimed at improving NRP and team performance is possible to implement in a rural setting. Our pilot study showed a trend toward improved NRP adherence, increased skill efficiency, and higher-quality teamwork and communication in one rural hospital. Additional research is needed to analyze program efficacy on a larger scale and to understand the impact of training on patient outcomes.
Key Points
Prevalence, Mortality, and Associated Anomalies in Esophageal Atresia: A Retrospective Study of Finnish Population Data (2004–2017)
Authors: Alikärri Suvi , Helenius Ilkka , Heiskanen Susanna , Syvänen Johanna , Kemppainen Teemu , Löyttyniemi Eliisa , Gissler Mika , Raitio Arimatias 
Published: September 2024

Abstract
Introduction The aim of the study was to investigate the prevalence of esophageal atresia (EA), frequency of associated anomalies, and mortality from 2004 to 2017 in the Finnish population. We hypothesized the Spitz classification and the presence of other congenital malformations would predict mortality in patients with EA as well as assumed the survival to be high among patients with EA.
Materials and Methods This retrospective, population-based study was based on the registries maintained by THL Finnish Institute for Health and Welfare and Statistics Finland. The cases were identified and classified according to the World Health Organization's International Classification of Diseases and Health Related Problems (ICD) revisions 9 and 10 (ICD-9 and ICD-10) codes and accompanying written diagnoses. Associated anomalies were classified based on the EUROCAT criteria, and minor anomalies were excluded. All statistical tests were performed as a two-sided significance level set at p < 0.05. The chi-square or Fisher's exact test was utilized for categorical variables. The change in prevalence rates during the study period was evaluated with linear regression.
Results In total, 337 cases with EA were identified including 295 (87.5%) live births, 17 (5.0%) stillbirths, and 25 (7.4%) terminations of pregnancy. The total prevalence for EA in Finland was 4.17/10,000 births with no significant change during the study period, p = 0.35. Neonatal mortality was 5% (n = 15) and 1-year survival was 91.5%. Mortality was associated with syndromic cases (p = 0.002). The Spitz classification predicted neonatal mortality better than cardiac anomalies alone (p < 0.001 and p = 0.6, respectively). Type C was the most common atresia type (65.9%) followed by type A (14.8%) and B (6.8%). The most common group of associated malformations were heart defects (35.0%) followed by other gastrointestinal tract malformations (15.3%) and limb anomalies (12.2%). Syndromic cases (12.2%) were associated with type A and B atresias (p = 0.001). VACTERL association was observed in 16.6% of the cases.
Conclusion The overall prevalence of EA remains stable and relatively high in Finland. Despite the high prevalence of co-occurring malformations, the overall survival rate is high. Spitz classification predicted neonatal survival well.
Telemedizinische Fränkische Adipositas NachsorgeTherapie (Tele-FANT)
– Ergebnisse der formativen Evaluation
Authors: Ahnert Jutta , Hart-Kederer Janina , Tischendorf Rainer , Vogel Heiner 
Published: September 2024

Zusammenfassung
Hintergrund Den Patient*innen gelingt es im Anschluss an eine
Rehabilitation oft nicht, die dort erlernten Verhaltensänderungen langfristig
weiterzuführen. Daher ist es wichtig, Nachsorgemaßnahmen anzubieten, um das
Selbstmanagement und den Transfer in den Alltag zu verbessern. Durch flexible
Einsatzmöglichkeiten und geringe Kosten bieten internetbasierte und telefonische
Nachsorgekonzepte die Chance, die Inanspruchnahme von Nachsorgemaßnahmen
deutlich zu erhöhen. Im Projekt „Tele-FANT“ wurden adipöse Patient*innen im
Anschluss an ihren stationären Reha-Aufenthalt über 12 Monate von einer
Nachsorgetherapeutin mittels regelmäßiger Telefon-/Video-Sprechstunde weiter
begleitet.
Methode Für die formative Evaluation wurde ein Eingruppen-Prä-Post-Design
genutzt. Es fand eine schriftliche Befragung der Teilnehmenden zu Reha-Beginn,
Reha-Ende sowie 4, 8 und 12 Monate nach der Rehabilitation statt. Als zentrale
Variable wurde ergänzend zu allen Messzeitpunkten das Körpergewicht
(Fremdmessung durch den*die Hausarzt*ärztin) zur Berechnung der prozentualen
Gewichtsabnahme von Reha-Beginn erhoben. Es wurden deskriptive Kennwerte, sowie
Mittelwertsdifferenzen und Standardized Effect Size (SES) zu den
Post-Messzeitpunkten berechnet.
Ergebnisse Es konnten 47 adipöse Rehabilitand*innen (mittlerer BMI=44,56,
SD=7,23) der Frankenlandklinik Bad Windsheim für die Teilnahme am
Nachsorgeprogramm gewonnen werden. Das komplette Programm bis zur
12-Monat-Katamnese durchliefen 30 Teilnehmende (Drop-Out: 36,2%). Für die
formalen Abläufe als auch die Inhalte des Nachsorgeprogramms zeigte sich eine
hohe Akzeptanz bei den verbleibenden Teilnehmenden. Eine Gewichtsabnahme um
mind. 10% wurde zur 12-Monats-Katamnese von 13 Teilnehmenden (50%) erreicht,
weitere 4 Teilnehmende (15,4%) haben 5 bis 9,99% abgenommen. Die Teilnehmenden
konnten den BMI seit Reha-Beginn durchschnittlich um insgesamt 4,45 BMI-Punkte
von 43,69 (SD=7,25) auf 39,24 (SD=7,25) reduzieren (mittlere Effektstärke:
SES=0,61).
Schlussfolgerung Das Nachsorgeprogramm „Tele-FANT“ zeigt in der formativen
Evaluation eine gute Machbarkeit und vielversprechende Ergebnisse bezüglich des
Ziels der Gewichtsreduktion/-stabilisierung. Die hohe Drop-Out-Rate weist
allerdings darauf hin, dass ein Teil der Teilnehmenden mit dem Programm nicht
zufrieden war oder nicht den gewünschten Erfolg erzielen konnte. Nächster
Schritt wäre, die Wirksamkeit des Nachsorgekonzepts „Tele-FANT“ in einer
randomisierten Kontrollgruppenstudie zu überprüfen.
In vitro Examination of the Bond Strength of PEEK and PEKK Materials Used as Substructure Materials with the Superstructure Composite before and after Artificial Aging and Examination of Scanning Electron Microscope Images
Authors: Kocer Merve Ustek , Apak Ayşe 
Published: September 2024

Abstract
Objective This study aims to evaluate the bond strength of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) materials when used as substructures with composite superstructures, before and after artificial aging. Surface treatments, including sanding and sandblasting, were examined to determine their impact on bond strength and surface topography.
Materials and Methods Sixty samples, divided equally between PEEK and PEKK, were prepared. Each group was further divided into three subgroups (n = 10): control, 10,000 thermal cycles, and 30,000 thermal cycles. The samples were subjected to surface treatments using 600-, 800-, and 1,200-grit silicon carbide wet sandpaper, followed by air abrasion with 110 µm alumina particles. Bond strength tests were conducted using an INSTRON-3345 universal testing machine, and failure types were analyzed under a stereomicroscope.
Results Sandblasting with 110 µm aluminum oxide (Al2O3) significantly increased the bond strength of both PEEK and PEKK materials compared with sanding. PEEK samples treated with sandblasting showed the highest bond strength (mean = 1.296 MPa), while PEKK samples treated with sanding had the lowest (mean = 0.056 MPa). Thermal cycling reduced bond strength in both materials, with a more pronounced decrease observed in the 30,000 cycle groups. analysis of variance results indicated significant differences in bond strength based on the material, surface treatment, and the interaction between these factors.
Conclusion Sandblasting with 110 µm Al2O3 is an effective method for enhancing the bond strength of PEEK and PEKK substructures with composite materials. These findings support the continued use of PEEK and PEKK polymers in dental applications, suggesting that appropriate surface treatments can significantly improve clinical outcomes.
Impact of Tonsillectomy and Associated Factors on Voice
Authors: Araki Naoto , Godo Shino , Hirano Yusuke , Fukase Ryo , Kurakami Kazuya , Sakurai Shinichi 
Published: September 2024

AbstractTonsillectomy is commonly performed to treat repetitive tonsillitis. Tonsillectomy changes the shape and characteristics of the pharynx. It could also alter the resonating characteristics of the pharynx and affect the voice of the patient. We examined voice changes in patients undergoing tonsillectomy. A total of 33 adult patients who underwent tonsillectomy at our institution between July 2020 and December 2021 were included in the study. The participants consisted of 16 men and 17 women, with a mean ± standard deviation age of 33.7 ± 10.7 years. We measured the maximum phonation time, acoustic analysis values (jitter%, shimmer%, and noise-to-harmonic ratio [NHR]), speaking fundamental frequency and vocal range, first four formants (F1–F4) in the sustained vowel /a/, and Voice Handicap Index (VHI). We examined whether any changes occurring 3 months after the surgery compared with the findings before the surgery. The shimmer% and NHR decreased significantly after surgery compared with the observations prior to surgery. Among women, the speaking fundamental frequency tended to increase, and the F2 significantly increased after the surgery compared with that prior to surgery. Removal of the swollen tonsils reduced the resistance of the supraglottic space and resulted in a lower laryngeal adduction force required for phonation. The decreases in the shimmer% and NHR were attributed to a reduction in the turbulence in the supraglottic space. The change in the shape of the pharynx caused changes in the speaking fundamental frequency and formants. The changes were thought to be influenced by the size of the patient's pharyngeal cavity, volume of the tonsils, and scarring of the pharyngeal mucosa. Patients should be informed before surgery of the possibility of voice changes.
Head of state immunity, order, justice and the international criminal court: limits of international criminal justice in international society
Authors: Killingsworth Matt 
Published: September 2024

AbstractThe post-Cold War international justice project is underpinned by the goal of overcoming impunity, an important part of which involves setting aside the historically evolved norm that rank or station will protect individuals from criminal responsibility. One of the primary forums where this plays out is the international criminal court. International relations theory is generally absent from discussions about the degree to which tensions between the legally evolved immunity for heads of state and the emerging idea that gross violations of human rights should trump claims of immunity, inform the relationship between order and justice. This paper proposes that the English School of International Relations provides the ideal framework to overcome this theoretical absence, and that in turn it is best suited to explain competing explanations of this tension, as it relates to order and justice, and broader limitations of the international criminal justice project.