bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of androgen deprivation therapy on sexual function and bother in men with prostate cancer: A controlled comparison
Authors: Donovan Kristine A. , Gonzalez Brian D. , Nelson Ashley M. , Fishman Mayer N. , Zachariah Babu , Jacobsen Paul B. 
Published: May 2017

AbstractObjectivesThe adverse sexual effects of androgen deprivation therapy (ADT) on men with prostate cancer have been well described. Less well known is the relative degree of sexual dysfunction and bother associated with ADT compared to other primary treatment modalities such as radical prostatectomy. We sought to describe the trajectory and relative magnitude of changes in sexual function and bother in men on ADT and to examine demographic and clinical predictors of ADT's adverse sexual effects.MethodsProstate cancer patients treated with ADT (n = 60) completed assessments of sexual function and sexual bother 3 times during a 1‐year period after the initiation of ADT. Prostate cancer patients treated with radical prostatectomy only and not receiving ADT (n = 85) and men with no history of cancer (n = 86) matched on age and education completed assessments at similar intervals.ResultsAndrogen deprivation therapy recipients reported worsening sexual function and increasing bother over time compared to controls. Effect sizes for the differences in sexual function were large to very large, and for bother were small to very large. Age younger than 83 years predicted relatively poorer sexual function, and age younger than 78 years predicted greater sexual bother at 12 months in men on ADT compared to men not on ADT.ConclusionsMost men on ADT for prostate cancer will never return to baseline levels of sexual function. Interventions focused on sexual bother over function and designed to help couples build and maintain satisfying relationship intimacy are likely to more positively affect men's psychological well‐being while on ADT than medical or sexual aids targeting sexual dysfunction.
Medical mishap as a cause of non‐resolving pneumonia
Authors: Toukan Yazeed , Gur Michal , Nir Vered , Bentur Lea 
Published: May 2017

AbstractA 15‐year old boy was referred for evaluation of fever, productive cough, and recurrent/persistent right lower lobe (RLL) pneumonia of 1‐month duration. Chest computed tomography scan showed RLL consolidation, with minimal pleural effusion and a liver abscess with sharp borders, containing a solid calcification in the lower portion. History revealed acute appendicitis with laparoscopic appendectomy 6 months prior to presentation, during which a fecalith dropped to the peritoneal cavity. Following laparoscopically guided drainage of the abscess the patient improved. Herein we describe a rare case of non‐resolving RLL pneumonia secondary to pyogenic liver abscess caused by dropped appendicolith.
Downregulation of androgen receptors by NaAsO2 via inhibition of AKT‐NF‐κB and HSP90 in castration resistant prostate cancer
Authors: Kim Yunlim , Park Sang Eun , Moon Jeong‐Weon , Kim Bong‐Min , Kim Ha‐Gyeong , Jeong In Gab , Yoo Sangjun , Ahn Jae Beom , You Dalsan , Pak Jhang Ho , Kim Sujong , Hwang Jung Jin , Kim Choung‐Soo 
Published: May 2017

BackgroundAndrogen and androgen receptor (AR) play essential roles in the development and maintenance of prostate cancer. The recently identified AR splice variants (AR‐Vs) have been considered as a plausible mechanism for the primary resistance against androgen deprivation therapy (ADT) in castration‐resistant prostate cancer (CRPC). Sodium meta‐arsenite (NaAsO2; KML001; Kominox), a trivalent arsenical, is an orally bioavailable and water soluble, which is currently in phase I/II clinical trials for the treatment of prostate cancer. It has a potent anti‐cancer effect on prostate cancer cells and xenografts. The aim of this study was to examine the effect of NaAsO2 on AR signaling in LNCaP and 22Rv1 CRPC cells.MethodsWe used hormone‐sensitive LNCaP cells, hormone‐insensitive 22Rv1 cells, and CRPC patient‐derived primary cells. We analyzed anti‐cancer effect of NaAsO2 using real‐time quantitative reverse transcription‐PCR, Western blotting, immunofluorescence staining and CellTiter Glo® luminescent assay. Statistical evaluation of the results was performed by one‐way ANOVA.ResultsNaAsO2 significantly reduced the translocation of AR and AR‐Vs to the nucleus as well as their level in LNCaP and 22Rv1 cells. Besides, the level of the prostate‐specific antigen (PSA), downstream target gene of AR, was also decreased. This compound was also an effective modulator of AKT‐dependent NF‐κB activation which regulates AR. NaAsO2 significantly inhibited phosphorylation of AKT and expression and nuclear translocation of NF‐κB. We then investigated the effect of NaAsO2 on AR stabilization. NaAsO2 promoted HSP90 acetylation by down‐regulating HDAC6, which reduces the stability of AR in prostate cancer cells.ConclusionsHere, we show that NaAsO2 disrupts AR signaling at multiple levels by affecting AR expression, stability, and degradation in primary tumor cell cultures from prostate cancer patients as well as CRPC cell lines. These results suggest that NaAsO2 could be a novel therapeutics for prostate cancer.
Success of capture of toads improved by manipulating acoustic characteristics of lures
Authors: Muller Benjamin J , Schwarzkopf Lin 
Published: May 2017

AbstractBACKGROUNDManagement of invasive vertebrates is a crucial component of conservation. Trapping reproductive adults is often effective for control, and modification of traps may greatly increase their attractiveness to such individuals. Cane toads (Rhinella marina) are invasive, and males use advertisement vocalisations to attract reproductive females. In amphibians, including toads, specific structural parameters of calls (e.g. dominant frequency and pulse rate) may be attractive to females. Some cane toad traps use an artificial advertisement vocalisation to attract toads. We determined whether variation of the call's parameters (volume, dominant frequency and pulse rate) could increase the capture rate of gravid females.RESULTSOverall, traps equipped with loud calls (80 dB at 1 m) caught significantly more toads, and proportionally more gravid females, than traps with quiet calls (60 dB at 1 m), and traps with low dominant frequency calls caught more gravid females than traps with median frequency calls. Traps with high pulse rate calls attracted more females than traps with low pulse rate calls. Approximately 91% of the females trapped using a low frequency and high pulse rate combination call were gravid, whereas in traps using a call with population median parameters only approximately 75% of captured females were gravid.CONCLUSIONCalls that indicated large‐bodied males (low frequency) with high energy reserves (high pulse rate) are often attractive to female anurans and were effective lures for female toads in our study. The design of future trapping regimes should account for behavioural preferences of the target sex. © 2017 Society of Chemical Industry
A qualitative and quantitative evaluation of the peptide characteristics of microwave‐ and ultrasound‐assisted digestion in discovery and targeted proteomic analyses
Authors: Guo Zhengguang , Cheng Jie , Sun Haidan , Sun Wei 
Published: May 2017

RationaleFast digestion methods can dramatically accelerate enzyme digestion and increase the throughput of proteomic analysis. However, the peptide characteristics of fast digestion methods and their performance in discovery and targeted proteomic analysis must be systematically evaluated.MethodsThree digestion methods, including overnight digestion, microwave‐assisted protein enzymatic digestion (MAPED), and high‐intensity focused ultrasonic‐assisted enzymatic digestion (HIFUSAED), in trypsin or in trypsin/Lys‐C were comprehensively compared in both discovery and targeted proteomics analysis using the HeLa cell proteome. In discovery proteomic analysis, the highest numbers of peptides and proteins were identified when the sample was digested via the MAPED method with trypsin/Lys‐C.ResultsThe fast digestion methods showed a higher mis‐cleavage rate and a lower semi‐tryptic rate than the overnight digestion method. In both label‐free quantitative analysis and targeted proteomic analysis, both fully cleaved peptides (FCPs) and mis‐cleaved peptides (MCPs) from the fast digestion methods and the overnight digestion method showed good reproducibility if they showed good abundance.ConclusionsWhen both the FCPs and MCPs were included in the analysis, the MAPED with trypsin/Lys‐C method showed the best results for both discovery proteomic analysis and relative quantitative targeted proteomic analysis. These results will be beneficial for the application of fast digestion methods to proteomics.
Collision‐induced dissociation of phenethylamides: role of ion‐neutral complexes
Authors: Nikolić Dejan , Macias Carmen , Lankin David C. , van Breemen Richard B. 
Published: May 2017

RationalePhenethylamides are a large group of naturally occurring molecules found both in the plant and animal kingdoms. In addition, they are used as intermediates for the synthesis of pharmaceutically important dihydro‐ and tetrahydroisoquinolines. To enable efficient characterization of this class of molecules, a detailed mass spectrometric fragmentation study of a broad series of analogs was carried out.MethodsThe test compounds were synthesized using standard methods for amide bond formation. Low‐energy high‐resolution tandem mass spectra were acquired on a hybrid quadrupole/time‐of‐flight mass spectrometer using positive ion electrospray ionization.ResultsA total of 26 analogs were investigated in the study. Fragmentation of phenethylamides was found to proceed via intermediate ion‐neutral complexes. The complexes can break down via multiple pathways including dissociation, proton transfer, Friedel‐Crafts acylation, and single electron transfer. The relative contribution of each of these pathways strongly depends on the structure of the coupling amine and acid.ConclusionsA general scheme for the fragmentation of phenethylamides was developed. This study further extends the knowledge base of the ion‐neutral complex by discovering Friedel‐Crafts acylation as a novel reaction. The strong influence of minor structural modifications on the fragmentation patterns highlights the importance of testing many analogs in order to fully predict a fragmentation pattern of a particular class of molecules.
Battle on the Wrong Field? Entrant Type, Dominant Designs, and Technology Exit
Authors: Chen Tianxu , Qian Lihong , Narayanan Vadake 
Published: May 2017

Research summary: Startups often compete with diversifying entrants in the technology race to define dominant designs, which can be platform technology‐based or non‐platform technology‐based. However, little research has examined the relative risk of technological exits for startups vs. diversifying entrants in such “dominance battles.” We develop a contingency framework that links a firm's technology exit to its pre‐entry experience and the characteristics of the dominance battle. With a sample of 134 technologies involved in 31 dominance battles in the information technology industry from 1979 to 2007, we show that technologies of startups were more likely than those of diversifying entrants to exit from platform technology‐based dominance battles; however, this relationship did not exist in non‐platform technology‐based dominance battles, or after the emergence of dominant designs.Managerial summary: How can a startup that tries to create a dominant design strategize to survive the fierce technology race? This study demonstrates that choosing the right battlefield is of paramount importance. Two aspects of a battlefield are shown as relevant: the type of technology and the stage of industrial evolution. Our results show that technologies sponsored by startups tend to have higher exit rates than those sponsored by diversifying entrants in dominance battles characterized by platform technologies, but this penalty is not evident in dominance battles characterized by non‐platform technologies or after the emergence of dominant designs. Furthermore, our study suggests that lack of organizational legitimacy, complementary assets, and integrative capabilities may explain why startups have a higher risk of technology exit than diversifying entrants.Copyright © 2017 John Wiley & Sons, Ltd.
Seismic retrofit of a high‐rise steel moment‐resisting frame using fluid viscous dampers
Authors: Wang Shanshan , Mahin Stephen A. 
Published: May 2017

SummaryThe Tall Building Initiative project of Pacific Earthquake Engineering Research Center has been expanded to investigate the seismic performance and possible retrofit of existing tall buildings. A candidate 35‐story steel building with representative details from the early 1970s was analyzed following several guidelines, which revealed a wide range of potential inadequacies. Thus, a two‐level retrofit approach was examined that focused on achieving the collapse prevention limit state under the major basic safety earthquake (BSE‐2E) hazard level prescribed by ASCE 41. This paper focused on a Level‐2 retrofit that used fluid viscous dampers to augment Level‐1 retrofits. For this approach, feasible damper locations and overall effective damping ratios were first evaluated through a series of preliminary studies, and then a two‐phase design method was used to refine the distribution and mechanical properties of the dampers. Thorough assessments of the refined design were carried out following several design guidelines, including ASCE 41, FEMA 351, and FEMA P‐58. The results indicated that the proposed retrofit method of using fluid viscous dampers could achieve the retrofit goal and provide a cost‐effective means of improving the structural behavior and reducing economic losses in a major seismic event.
Four‐dimensional ultrasound guidance for pelvic floor Botulinum toxin‐A injection in chronic pelvic pain: a novel technique
Authors: Nesbitt‐Hawes E. M. , Dietz H. P. , Abbott J. A. 
Published: May 2017

ABSTRACTObjectiveBotulinum toxin‐A (BoNT‐A) is used in the treatment of pelvic floor muscle overactivity associated with chronic pelvic pain (CPP) when conservative methods such as physiotherapy are not effective or appropriate. Traditional injection techniques require finger‐guided palpation of pelvic floor muscles with concurrent insertion of the needle. The aim of this study was to describe a novel technique for the injection of BoNT‐A into the pelvic floor musculature using four‐dimensional ultrasound (4D‐US) guidance.MethodsThirty‐one BoNT‐A injections were performed using the new technique between October 2013 and January 2016, on women scheduled to have BoNT‐A injection for pelvic floor muscle overactivity and CPP. The pelvic floor was assessed by 4D‐US. A test injection of saline was performed to confirm location of the needle, then BoNT‐A was injected into the muscle under ultrasound guidance, using 4D‐US to confirm that the fluid expanded and tracked along muscle fibers.ResultsThe saline test confirmed correct location of the needle following a median of 1 (range, 1–3) attempt at needle placement. In all 31 instances, satisfactory injection of BoNT‐A, with 4D‐US confirmation of fluid expansion within the muscle body, was performed.ConclusionsInjection of BoNT‐A under 4D‐US guidance is feasible and allows accurate placement into the target muscle in women with pelvic floor muscle overactivity associated with CPP. This technique may provide a safer alternative to finger‐guided methods, owing to a lower likelihood of operator needle‐stick injury. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.
Effect of increasing fruit and vegetable intake by dietary intervention on nutritional biomarkers and attitudes to dietary change: a randomised trial
Authors: Duthie Susan J. , Duthie Garry G. , Russell Wendy R. , Kyle Janet A. M. , Macdiarmid Jennie I. , Rungapamestry Vanessa , Stephen Sylvia , Megias-Baeza Cristina , Kaniewska Joanna J. , Shaw Lindsey , Milne Lesley , Bremner David , Ross Karen , Morrice Philip , Pirie Lynn P. , Horgan Graham , Bestwick Charles S. 
Published: May 2017

AbstractPurposeLow fruit and vegetable consumption is linked with an increased risk of death from vascular disease and cancer. The benefit of eating fruits and vegetables is attributed in part to antioxidants, vitamins and phytochemicals. Whether increasing intake impacts on markers of disease remains to be established. This study investigates whether increasing daily intake of fruits, vegetables and juices from low (approx. 3 portions), to high intakes (approx. 8 portions) impacts on nutritional and clinical biomarkers. Barriers to achieving the recommended fruit and vegetable intakes are also investigated.MethodIn a randomised clinical trial, the participants [19 men and 26 women (39–58 years)] with low reported fruit, juice and vegetable intake (<3 portions/day) were randomised to consume either their usual diet or a diet supplemented with an additional 480 g of fruit and vegetables and fruit juice (300 ml) daily for 12 weeks. Nutritional biomarkers (vitamin C, carotenoids, B vitamins), antioxidant capacity and genomic stability were measured pre-intervention, at 4-, 8- and 12 weeks throughout the intervention. Samples were also taken post-intervention after a 6-week washout period. Glucose, homocysteine, lipids, blood pressure, weight and arterial stiffness were also measured. Intake of fruit, fruit juice and vegetables was reassessed 12 months after conducting the study and a questionnaire was developed to identify barriers to healthy eating.ResultsIntake increased significantly in the intervention group compared to controls, achieving 8.4 portions/day after 12 weeks. Plasma vitamin C (35%), folate (15%) and certain carotenoids [α-carotene (50%) and β-carotene (70%) and lutein/zeaxanthin (70%)] were significantly increased (P < 0.05) in the intervention group. There were no significant changes in antioxidant capacity, DNA damage and markers of vascular health. Barriers to achieving recommended intakes of fruits and vegetables measured 12 months after the intervention period were amount, inconvenience and cost.ConclusionWhile increasing fruit, juice and vegetable consumption increases circulating level of beneficial nutrients in healthy subjects, a 12-week intervention was not associated with effects on antioxidant status or lymphocyte DNA damage.Trial registrationThis trial was registered at Controlled-Trials.com; registration ISRCTN71368072.
Identification and characterization of microRNAs and their targets in high-altitude stress-adaptive plant maca (Lepidium meyenii Walp)
Authors: Paul Sujay 
Published: May 2017

Abstract
MicroRNAs (miRNAs) are endogenous, short (~21-nucleotide), non-coding RNA molecules that play pivotal roles in plant growth, development, and stress response signaling. In this study using recently published draft genome sequence of a high-altitude plant maca (Lepidium meyenii Walp) and applying genome-wide computational-based approaches, a total of 62 potentially conserved miRNAs belonging to 28 families were identified and four (lme-miR160a, lme-miR164c, lme-miR 166a, and lme-miR 319a) of them further validated by RT-PCR. Deploying psRNATarget tool a total of 99 potential miRNA target transcripts were also identified in maca. Targets include a number of transcription factors like Squamosa promoter-binding, NAC, MYB, auxin response factor, APETALA, WRKY, and F-box protein. To the best of my knowledge, this is the first genome-based miRNA profiling of a high-altitude plant.
Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes
Authors: Sathya Arumugam , Vijayabharathi Rajendran , Gopalakrishnan Subramaniam 
Published: May 2017

AbstractGrain legumes are a cost-effective alternative for the animal protein in improving the diets of the poor in South-East Asia and Africa. Legumes, through symbiotic nitrogen fixation, meet a major part of their own N demand and partially benefit the following crops of the system by enriching soil. In realization of this sustainability advantage and to promote pulse production, United Nations had declared 2016 as the “International Year of pulses”. Grain legumes are frequently subjected to both abiotic and biotic stresses resulting in severe yield losses. Global yields of legumes have been stagnant for the past five decades in spite of adopting various conventional and molecular breeding approaches. Furthermore, the increasing costs and negative effects of pesticides and fertilizers for crop production necessitate the use of biological options of crop production and protection. The use of plant growth-promoting (PGP) bacteria for improving soil and plant health has become one of the attractive strategies for developing sustainable agricultural systems due to their eco-friendliness, low production cost and minimizing consumption of non-renewable resources. This review emphasizes on how the PGP actinobacteria and their metabolites can be used effectively in enhancing the yield and controlling the pests and pathogens of grain legumes.
Synergistic effect of Chryseobacterium gleum sp. SUK with ACC deaminase activity in alleviation of salt stress and plant growth promotion in Triticum aestivum L.
Authors: Bhise Komal K. , Bhagwat Prashant K. , Dandge Padma Babulal 
Published: May 2017

Abstract
Soil salinity is major abiotic stresses affecting morphological, biochemical and physiological processes of plant growth. Chryseobacterium gleum sp. SUK isolated from salt-stressed soil exhibited ACC (1-aminocyclopropane-1-carboxylate) deaminase activity with IAA (indole acetic acid), siderophore, ammonia, hydrogen cyanide production, 2% salt tolerance and fungal cell wall degrading enzyme production (cellulase, protease). The isolate also showed a poultry feather degrading activity which is the main waste material of poultry industry and opulent source of proteins, amino acids, nitrogen, phosphorous, calcium, potassium, manganese, zinc and copper. Application of feather-degraded lysate with the degrading isolate, C. gleum sp. SUK denotes triple role of bioformulation to surmount salinity stress, management of poultry waste disposal and utilization of feathers degraded products as a biostimulant for better growth of plants as well as strain SUK having multifarious plant growth promoting traits. Wheat crops exposed to salt stressor were inoculated with studied bioformulation. Results of plant analysis showed improvement in root and shoot length, fresh and dry weight, chlorophyll, proteins, amino acids, phenolics, flavonoids content and decreased level of proline. In addition, Na+ uptake was decreased and K+ uptake was increased. Therefore, application of novel bioformulation could increase the yield of crops by ameliorating growth of plants and alleviating the salinity stress.