bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration
Authors: Qi Yiying , Niu Lie , Zhao Tengfei , Shi Zhongli , Di Tuoyu , Feng Gang , Li Junhua , Huang Zhongming 
Published: December 2015

Abstract
Background
Promotion of bone regeneration is important for successful repair of bony defects. This study aimed to investigate whether combining bone marrow-derived mesenchymal stem cell (BMSC) sheets with platelet-rich plasma (PRP) gel/calcium phosphate particles could promote bone formation in the femoral bone defects of rats.

Methods
The proliferation and differentiation of BMSCs or BMSC sheets cultured with calcium phosphate particles and/or PRP were investigated in in vitro. In vivo, 36 2.5 × 5 mm bone defects were randomly divided into groups and treated with either BMSCs/PRP gel, calcium phosphate particles, PRP gel/calcium phosphate particles, a BMSC sheet/calcium phosphate particles, a BMSC sheet/PRP gel/calcium phosphate particles, or were left untreated (n = 6/group). A further 15 bone defects were treated with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil)-labelled BMSC sheet/PRP gel/calcium phosphate particles and observed using a small animal in vivo fluorescence imaging system to trace the implanted BMSCs at 1 day, 3 days, 7 days, 2 weeks, and 4 weeks after surgery.

Results
The expression of collagen type I and osteocalcin genes of BMSCs or BMSC sheets treated with PRP and calcium phosphate particles was significantly higher than that of BMSCs or BMSC sheets treated with calcium phosphate particles or the controls (P <0.05). PRP can promote gene expression of collagen III and tenomodulin by BMSCs and in BMSC sheets. The VEGF, collagen I and osteocalcin gene expression levels were higher in the BMSC sheet than in cultured BMSCs (P <0.05). Moreover, alizarin red staining quantification, ALP quantification and calcein blue fluorescence showed the osteogenic potential of BMSCs treated with PRP and calcium phosphate particles The implanted BMSCs were detectable at 1 day, 3 days, 7 days, 2 weeks and 4 weeks after surgery by a small animal in vivo fluorescence imaging system and were visualized in the defect zones by confocal microscopy. At 4 weeks after implantation, the defects treated with the BMSC sheet/PRP gel/calcium phosphate particles showed significantly more bone formation than the other five groups.

Conclusions
Incorporation of an BMSC sheet into the PRP gel/calcium phosphate particles greatly promoted bone regeneration. These BMSC sheet and tissue engineering strategies offer therapeutic opportunities for promoting bone defect repair clinically.
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint
Authors: O'Connor Aisling , Maffini Stefano , Rainey Michael D. , Kaczmarczyk Agnieszka , Gaboriau David , Musacchio Andrea , Santocanale Corrado 
Published: December 2015

ABSTRACT
During mitotic arrest induced by microtubule targeting drugs, the weakening of the spindle assembly checkpoint (SAC) allows cells to progress through the cell cycle without chromosome segregation occurring. PLK1 kinase plays a major role in mitosis and emerging evidence indicates that PLK1 is also involved in establishing the checkpoint and maintaining SAC signalling. However, mechanistically, the role of PLK1 in the SAC is not fully understood, with several recent reports indicating that it can cooperate with either one of the major checkpoint kinases, Aurora B or MPS1. In this study, we assess the role of PLK1 in SAC maintenance. We find that in nocodazole-arrested U2OS cells, PLK1 activity is continuously required for maintaining Aurora B protein localisation and activity at kinetochores. Consistent with published data we find that upon PLK1 inhibition, phosphoThr3-H3, a marker of Haspin activity, is reduced. Intriguingly, Aurora B inhibition causes PLK1 to relocalise from kinetochores into fewer and much larger foci, possibly due to incomplete recruitment of outer kinetochore proteins. Importantly, PLK1 inhibition, together with partial inhibition of Aurora B, allows efficient SAC override to occur. This phenotype is more pronounced than the phenotype observed by combining the same PLK1 inhibitors with partial MPS1 inhibition. We also find that PLK1 inhibition does not obviously cooperate with Haspin inhibition to promote SAC override. These results indicate that PLK1 is directly involved in maintaining efficient SAC signalling, possibly by cooperating in a positive feedback loop with Aurora B, and that partially redundant mechanisms exist which reinforce the SAC.
Nanostructure for Hybrid Plasmonic–Photonic Crystal Formed on Gel-Immobilized Colloidal Crystal Observed by AFM after Drying
Authors: Kawakami Sho , Mori Atsushi , Nagashima Ken , Hashimoto Shuichi , Haraguchi Masanobu 
Published: December 2015

Abstract
Aiming at fabrication of hybrid plasmonic–photonic crystals, gel-immobilized colloidal crystals made of a polystyrene colloidal suspension and an N-(hydroxymethyl)acrylamide-based gel were immersed into an aqueous dispersion of gold nanoparticles. Atomic force microscope (AFM) observations have been performed for the gel-immobilized colloidal crystals with gold nanoparticles deposited on their surfaces. In the present study, the diameter of a colloidal sphere was ca. 190 nm. The diameter of a gold nanoparticle was the same as in a preliminary study, ca. 40 nm. Various immersion times up to two hours were tested. Surface of a sample of 2 h immersion has been observed. Prior to the AFM observation, the sample was dried in a desiccator for 18 h. We have identified a face-centered cubic {111} structure of a colloidal crystal of nearly close packing. Nanoparticles isolated with one another have been observed on the surface of gel-immobilized crystal, which can be regarded as gold nanoparticles from their sizes.
Family and schizophrenia - psychoeducational group in a pilot programme
Authors: Kordas Wojciech , Kokodyńska Katarzyna , Kurtyka Agnieszka , Sikorska Iwona , Walczewski Krzysztof , Bogacz Józef 
Published: December 2015

CelCelem artykułu jest prezentacja przebiegu, wyników i wniosków z przeprowadzonego cyklu zajęć psychoedukacyjnych dla rodzin osób chorych na schizofrenię, leczących się w Szpitalu Specjalistycznym im. dr J. Babińskiego w Krakowie. Artykuł prezentuje projekt o charakterze eksploracyjnym, opierający się na jakościowym podejściu badawczym. Prezentowany program psychoedukacyjny mieści się w systemowym podejściu terapeutycznym, wykorzystującym ujęcie narracyjne.MetodaW projekcie wzięło udział 23 osoby z 16 rodzin, które uczestniczyły w 14 spotkaniach psychoedukacyjnych na przestrzeni 4 miesięcy. Zastosowano różnorodne metody edukacyjne: wykłady, studium przypadku, warsztaty interaktywne, psychorysunek, prezentacje multimedialne, symulacje sytuacji kryzysowych, zajęcia w podgrupach.WynikiPo zakończeniu cyklu spotkań u uczestników nie stwierdzono wyraźnego wzrostu poziomu wiedzy teoretycznej o schizofrenii, odnotowano natomiast spadek subiektywnie odczuwanego poczucia obciążenia chorobą w rodzinie. Mimo że wstępnie jedynie 11% uczestników oczekiwało od grupy wsparcia emocjonalnego, przez 60% badanych wsparcie zostało uznane za najważniejszą korzyść wyniesioną z programu.WnioskiZastosowanie w programie systemowego podejścia narracyjnego spotkało się z pozytywnym przyjęciem i dobrą oceną przez rodziny pacjentów chorujących na schizofrenię. Wydaje się być efektywną alternatywną dla „tradycyjnej” psychoedukacji. Wykorzystanie skutecznych narzędzi szkoleniowych połączone z jednoczesnym zostawieniem dużej przestrzeni do wymiany myśli i doświadczeń sprawdziło się jako dobra forma pomocy rodzinie w kryzysie.
Importance of integrating a cultural module in the community nursing curriculum
Authors: Al-Shdayfat Noha , Hasna Fadia , Al-Smairan Mohammad , Lewando-Hundt Gillian , Shudayfat Tamador 
Published: December 2015

Introduction:The Bedouin Health module was one of the proposed interventions funded by the European Union in 2007–2008, with a focus on health services and utilisation of these services for the Bedouin people in the north of Jordan.Aim:This study aimed to evaluate the sustainability of integrating this module into the community health nursing courses at Al al-Bayt University, Jordan.Method:Two focus group discussions were carried out with five faculty members and five students involved in the implementation of the Bedouin Health module. Leading questions were prepared for both groups.Results:Four main themes emerged across both focus groups: self-expression, sustainability, acceptance, and motivation.Conclusion:The findings of this study identify how crucial it is for nursing students and faculty members to be culturally competent when practising community health care in the Bedouin community.
Unintended Pregnancy in the Native Hawaiian Community: Key Informants’ Perspectives
Authors: Soon Reni , Elia Jennifer , Beckwith Nina , Kaneshiro Bliss , Dye Timothy 
Published: December 2015

CONTEXTNative Hawaiians experience the highest reported rate of unintended pregnancy of any ethnic group in Hawaii. Understanding the context in which they make decisions that influence pregnancy and pregnancy planning is essential to reducing this rate.METHODSA qualitative study was carried out in partnership with a community health center serving a large Native Hawaiian population to explore how Native Hawaiians conceptualize pregnancy and pregnancy planning. Between August and October 2013, semistructured interviews were conducted with 10 diverse key informants from the Hawaiian community. Content analysis was used to identify themes and patterns that emerged from the interviews.RESULTSCore Hawaiian values of children and family strongly affect how Native Hawaiians view pregnancy, pregnancy planning and unintended pregnancies. ‘Ohana (families) are large and characterized by tremendous support, which is perceived to lessen the burden of an unintended pregnancy. Pregnancies, whether planned or not, are seen as blessings because children are highly valued. Because of these concepts, there is an expectation for women to continue unplanned pregnancies. Although Hawaiians ascribe value to planning pregnancies and hope that children are born under what they identify as ideal circumstances, they acknowledge that these circumstances are not necessary and often do not occur.CONCLUSIONThe concepts of family and children serve as core values to Native Hawaiians and are linked to the ways in which they view pregnancy and pregnancy planning.
What sort of novel is The Wire? Voice, dialogue and protest
Authors: Stacy Ivan 
Published: December 2015

Abstract
This article responds to the numerous comparisons between The Wire and realist or naturalist novels. It argues that The Wire’s mimetic qualities depict many of the problems facing Baltimore and, by extension, neo-liberal America. However, it argues that the show does not just articulate complaints, but also proposes solutions, and that these solutions can be identified through two other concepts associated with the novel: polyphony and minor literature. The article uses Mikhail Bakhtin’s concept of polyphony to examine that way that different voices compete and interact in the series, but notes that, where the relationship between discourses remains conflictual, long-lasting or fundamental change remains unlikely. The article suggests that where more significant change does take place is through the creation of the collective voices associated with Gilles Deleuze and Félix Guattari’s concept of minor literature.
Reviews
Authors: Giannakopoulou Liana , Assinder Semele , Mackridge Peter , Fernández Zoa Alonso 
Published: December 2015

Abstract
K.P. KAVAFIS, TA THEATRIKA POIIMATA/THE THEATRICAL POEMS, Maria Athanasopoulou (2014) Athens: Kritiki, 154 pp, ISBN: 9789602189214, Paperback, €10.00
Greece and Britain since 1945, David Wills (ed.) (2014) Second Edition, Newcastle: Cambridge Scholars, 213 pp, ISBN: 9781443855341, Paperback, £29.99
The Ghost Behind the Arras: Transformat ions of the ‘POLITICAL Verse’ in Twentieth-century Greek Poetry, Vassilios Letsios (2013) Saarbrücken: Scholar’s Press, 399 pp, ISBN: 3639511441, Paperback, £97.00
Ludics in Surrealist Theatre and Beyond, Vassiliki Rapti (2013) Burlington: Ashgate Studies in Surrealism, xiv + 193 pp, ISBN: 9781409429067, Hardback, $99.95
Unsettling the metaphor: The failure of liberal hybridity in Fatih Akin’s Crossing The Bridge
Authors: Özselçuk Evren 
Published: December 2015

Abstract
This article examines Fatih Akin’s documentary Crossing the Bridge: The Sound of Istanbul, which focuses on the contemporary music scene in the city. It argues that through its narrative structure and narrator’s voice, the documentary latently reproduces Orientalizing assumptions and frameworks and ends up undermining the understanding of hybridity that it purportedly promotes as a boundary-blurring process of cross-fertilization. Strikingly, the documentary’s liberal-pluralist interpretation of the bridge in Istanbul as a metaphor for harmonious amalgamation and/or transculturation also collapses halfway through the film. The bridge, instead, transforms into a boundary-marking device which, as it renders Turkey Europe’s periphery, at the same time promises the European subject an imaginary voyage over space and time that enables his access to an ‘authenticity’ untouched by modernity. The article concludes with an analysis of the accounts of two particular participants in the documentary which reveal the limits of both the liberal-pluralist and nationalist appropriations of the bridge as a metaphor for Turkish identity and culture.
Dissolution behavior and cell compatibility of alkali-free MgO-CaO-SrO-TiO2-P2O5 glasses for biomedical applications
Authors: Lee Sungho , Obata Akiko , Brauer Delia S. , Kasuga Toshihiro 
Published: December 2015

AbstractOwing to their controlled solubility, phosphate invert glasses are of interest for use as temporary implant materials or tissue engineering scaffolds for controlled ion release.MgO-CaO-SrO-TiO2-P2O5 invert glasses were prepared and their dissolution behavior and cell response were examined.MgO addition to the phosphate invert glass system improved glass formation, owing to the relatively large field strength of Mg2+ ions. In osteoblastlike MC3T3-E1 cell culture tests, cell numbers on the invert glasses were significantly larger compared with the control, possibly caused by the release of Mg2+ ions promoting enhanced cell adhesion and proliferation. Alkaline phosphatase (ALP) activity varied with glass composition, with higher strontium for calcium substitution (33 to 100%) showing highest ALP activity. This effect may be caused by the release of strontium ions from the glasses.
Concise gene signature for point‐of‐care classification of tuberculosis
Authors: Maertzdorf Jeroen , McEwen Gayle , Weiner January , Tian Song , Lader Eric , Schriek Ulrich , Mayanja‐Kizza Harriet , Ota Martin , Kenneth John , Kaufmann Stefan HE 
Published: December 2015

AbstractThere is an urgent need for new tools to combat the ongoing tuberculosis (TB) pandemic. Gene expression profiles based on blood signatures have proved useful in identifying genes that enable classification of TB patients, but have thus far been complex. Using real‐time PCR analysis, we evaluated the expression profiles from a large panel of genes in TB patients and healthy individuals in an Indian cohort. Classification models were built and validated for their capacity to discriminate samples from TB patients and controls within this cohort and on external independent gene expression datasets. A combination of only four genes distinguished TB patients from healthy individuals in both cross‐validations and on separate validation datasets with very high accuracy. An external validation on two distinct cohorts using a real‐time PCR setting confirmed the predictive power of this 4‐gene tool reaching sensitivity scores of 88% with a specificity of around 75%. Moreover, this gene signature demonstrated good classification power in HIV+ populations and also between TB and several other pulmonary diseases. Here we present proof of concept that our 4‐gene signature and the top classifier genes from our models provide excellent candidates for the development of molecular point‐of‐care TB diagnosis in endemic areas.
Patient-reported outcomes of parenteral somatostatin analogue injections in 195 patients with acromegaly
Authors: Strasburger Christian J , Karavitaki Niki , Störmann Sylvère , Trainer Peter J , Kreitschmann-Andermahr Ilonka , Droste Michael , Korbonits Márta , Feldmann Berit , Zopf Kathrin , Sanderson Violet Fazal , Schwicker David , Gelbaum Dana , Haviv Asi , Bidlingmaier Martin , Biermasz Nienke R 
Published: December 2015

BackgroundLong-acting somatostatin analogues delivered parenterally are the most widely used medical treatment in acromegaly. This patient-reported outcomes survey was designed to assess the impact of chronic injections on subjects with acromegaly.MethodsThe survey was conducted in nine pituitary centres in Germany, UK and The Netherlands. The questionnaire was developed by endocrinologists and covered aspects of acromegaly symptoms, injection-related manifestations, emotional and daily life impact, treatment satisfaction and unmet medical needs.ResultsIn total, 195 patients participated, of which 112 (57%) were on octreotide (Sandostatin LAR) and 83 (43%) on lanreotide (Somatuline Depot). The majority (>70%) of patients reported acromegaly symptoms despite treatment. A total of 52% of patients reported that their symptoms worsen towards the end of the dosing interval. Administration site pain lasting up to a week following injection was the most frequently reported injection-related symptom (70% of patients). Other injection site reactions included nodules (38%), swelling (28%), bruising (16%), scar tissue (8%) and inflammation (7%). Injection burden was similar between octreotide and lanreotide. Only a minority of patients received injections at home (17%) and 5% were self-injecting. Over a third of patients indicated a feeling of loss of independence due to the injections, and 16% reported repeated work loss days. Despite the physical, emotional and daily life impact of injections, patients were satisfied with their treatment, yet reported that modifications that would offer major improvement over current care would be ‘avoiding injections’ and ‘better symptom control’.ConclusionLifelong injections of long-acting somatostatin analogues have significant burden on the functioning, well-being and daily lives of patients with acromegaly.
Quantitative Trait Locus and Genetical Genomics Analysis Identifies Putatively Causal Genes for Fecundity and Brooding in the Chicken
Authors: Johnsson Martin , Jonsson Kenneth B , Andersson Leif , Jensen Per , Wright Dominic 
Published: December 2015

Abstract
Life history traits such as fecundity are important to evolution because they make up components of lifetime fitness. Due to their polygenic architectures, such traits are difficult to investigate with genetic mapping. Therefore, little is known about their molecular basis. One possible way toward finding the underlying genes is to map intermediary molecular phenotypes, such as gene expression traits. We set out to map candidate quantitative trait genes for egg fecundity in the chicken by combining quantitative trait locus mapping in an advanced intercross of wild by domestic chickens with expression quantitative trait locus mapping in the same birds. We measured individual egg fecundity in 232 intercross chickens in two consecutive trials, the second one aimed at measuring brooding. We found 12 loci for different aspects of egg fecundity. We then combined the genomic confidence intervals of these loci with expression quantitative trait loci from bone and hypothalamus in the same intercross. Overlaps between egg loci and expression loci, and trait–gene expression correlations identify 29 candidates from bone and five from hypothalamus. The candidate quantitative trait genes include fibroblast growth factor 1, and mitochondrial ribosomal proteins L42 and L32. In summary, we found putative quantitative trait genes for egg traits in the chicken that may have been affected by regulatory variants under chicken domestication. These represent, to the best of our knowledge, some of the first candidate genes identified by genome-wide mapping for life history traits in an avian species.
Formation of Extrachromosomal Circular DNA from Long Terminal Repeats of Retrotransposons inSaccharomyces cerevisiae
Authors: Møller Henrik D , Larsen Camilla E , Parsons Lance , Hansen Anders Johannes , Regenberg Birgitte , Mourier Tobias 
Published: December 2015

AbstractExtrachromosomal circular DNA (eccDNA) derived from chromosomal Ty retrotransposons in yeast can be generated in multiple ways. Ty eccDNA can arise from the circularization of extrachromosomal linear DNA during the transpositional life cycle of retrotransposons, or from circularization of genomic Ty DNA. Circularization may happen through nonhomologous end-joining (NHEJ) of long terminal repeats (LTRs) flanking Ty elements, by Ty autointegration, or by LTR–LTR recombination. By performing an in-depth investigation of sequence reads stemming from Ty eccDNAs obtained from populations of Saccharomyces cerevisiae S288c, we find that eccDNAs predominantly correspond to full-length Ty1 elements. Analyses of sequence junctions reveal no signs of NHEJ or autointegration events. We detect recombination junctions that are consistent with yeast Ty eccDNAs being generated through recombination events within the genome. This opens the possibility that retrotransposable elements could move around in the genome without an RNA intermediate directly through DNA circularization.
Brain Based Teaching Model as Transformation of Learning Paradigm in Higher Education
Authors: Sesmiarni Zulfani 
Published: December 2015

Brain -based teaching model is a new paradigm that can facilitate students in optimizing student learning by the functioning the brain as a whole. Lessons are held today assume that all students equally so that learning provide the same services to each student in the class. With this model, the students are given different stimulation according to their abilities and needs. Base on brain learning theory -based teaching, the learning should pay attention to the five needs of the brain in general. The fifth factor is the need for a sense of comfort, the need for interaction, the need for knowledge, the need for the activity and the need for self-reflection. All these needs will be connected if the lecturers able to present emotional learning, social learning, cognitive learning, physical learning and teaching reflection. Key Word : Instrucetional, Brain Based teaching, Learning.Copyright © 2015 by Al-Ta'lim All right reserved