bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arhgap17, a RhoGTPase activating protein, regulates mucosal and epithelial barrier function in the mouse colon
Authors: Lee So-young , Kim Hwain , Kim Kyoungmi , Lee Hyunji , Lee Seungbok , Lee Daekee 
Published: May 2016

AbstractCoordinated regulation of the actin cytoskeleton by the Rho GTPase family is required for the maintenance of polarity in epithelial cells as well as for their proliferation and migration. A RhoGTPase-activating protein 17 (Arhgap17) is known to be involved in multiple cellular processes in vitro, including the maintenance of tight junctions and vesicle trafficking. However, the function of Arhgap17 has not been studied in the physiological context. Here, we generated Arhgap17-deficient mice and examined the effect in the epithelial and mucosal barriers of the intestine. Reporter staining revealed that Arhgap17 expression is limited to the luminal epithelium of intestine. Arhgap17-deficient mice show an increased paracellular permeability and aberrant localization of the apical junction complex in the luminal epithelium, but do not develop spontaneous colitis. The inner mucus layer is impervious to the enteric bacteria irrespective of Tff3 downregulation in the Arhgap17-deficient mice. Interestingly however, treatment with dextran sulfate sodium (DSS) causes an increased accumulation of DSS and TNF production in intraluminal cells and rapid destruction of the inner mucus layer, resulting in increased severity of colitis in mutant mice. Overall, these data reveal that Arhgap17 has a novel function in regulating transcellular transport and maintaining integrity of intestinal barriers.
Nicotinamide Riboside Opposes Type 2 Diabetes and Neuropathy in Mice
Authors: Trammell Samuel A.J. , Weidemann Benjamin J. , Chadda Ankita , Yorek Matthew S. , Holmes Amey , Coppey Lawrence J. , Obrosov Alexander , Kardon Randy H. , Yorek Mark A. , Brenner Charles 
Published: May 2016

AbstractMale C57BL/6J mice raised on high fat diet (HFD) become prediabetic and develop insulin resistance and sensory neuropathy. The same mice given low doses of streptozotocin are a model of type 2 diabetes (T2D), developing hyperglycemia, severe insulin resistance and diabetic peripheral neuropathy involving sensory and motor neurons. Because of suggestions that increased NAD+ metabolism might address glycemic control and be neuroprotective, we treated prediabetic and T2D mice with nicotinamide riboside (NR) added to HFD. NR improved glucose tolerance, reduced weight gain, liver damage and the development of hepatic steatosis in prediabetic mice while protecting against sensory neuropathy. In T2D mice, NR greatly reduced non-fasting and fasting blood glucose, weight gain and hepatic steatosis while protecting against diabetic neuropathy. The neuroprotective effect of NR could not be explained by glycemic control alone. Corneal confocal microscopy was the most sensitive measure of neurodegeneration. This assay allowed detection of the protective effect of NR on small nerve structures in living mice. Quantitative metabolomics established that hepatic NADP+ and NADPH levels were significantly degraded in prediabetes and T2D but were largely protected when mice were supplemented with NR. The data justify testing of NR in human models of obesity, T2D and associated neuropathies.
Mechanism of impaired microtubule-dependent peroxisome trafficking and oxidative stress in SPAST-mutated cells from patients with Hereditary Spastic Paraplegia
Authors: Wali Gautam , Sutharsan Ratneswary , Fan Yongjun , Stewart Romal , Tello Velasquez Johana , Sue Carolyn M , Crane Denis I. , Mackay-Sim Alan 
Published: May 2016

AbstractHereditary spastic paraplegia (HSP) is an inherited neurological condition that leads to progressive spasticity and gait abnormalities. Adult-onset HSP is most commonly caused by mutations in SPAST, which encodes spastin a microtubule severing protein. In olfactory stem cell lines derived from patients carrying different SPAST mutations, we investigated microtubule-dependent peroxisome movement with time-lapse imaging and automated image analysis. The average speed of peroxisomes in patient-cells was slower, with fewer fast moving peroxisomes than in cells from healthy controls. This was not because of impairment of peroxisome-microtubule interactions because the time-dependent saltatory dynamics of movement of individual peroxisomes was unaffected in patient-cells. Our observations indicate that average peroxisome speeds are less in patient-cells because of the lower probability of individual peroxisome interactions with the reduced numbers of stable microtubules: peroxisome speeds in patient cells are restored by epothilone D, a tubulin-binding drug that increases the number of stable microtubules to control levels. Patient-cells were under increased oxidative stress and were more sensitive than control-cells to hydrogen peroxide, which is primarily metabolised by peroxisomal catalase. Epothilone D also ameliorated patient-cell sensitivity to hydrogen-peroxide. Our findings suggest a mechanism for neurodegeneration whereby SPAST mutations indirectly lead to impaired peroxisome transport and oxidative stress.
Isolation of the Fathead Minnow Nidovirus from Muskellunge Experiencing Lingering Mortality
Authors: Faisal Mohamed , Baird Ashley , Winters Andrew D. , Millard Elena V. , Marcquenski Sue , Hsu Hui-Min , Hennings Ann , Bochsler Phil , Standish Isaac , Loch Thomas P. , Gunn Michelle R. , Warg Janet 
Published: May 2016

Abstract
In 2011, the Fathead Minnow nidovirus (FHMNV; Genus Bafinivirus, Family Coronaviridae, Order Nidovirales) was isolated from pond-raised juvenile Muskellunge Esox masquinongy suffering from lingering mortality at the Wild Rose Hatchery in Wild Rose, Wisconsin. Moribund Muskellunge exhibited tubular necrosis in the kidneys as well as multifocal coalescing necrotizing hepatitis. The FHMNV was also isolated from apparently healthy juvenile Muskellunge at the Wolf Lake State Fish Hatchery in Mattawan, Michigan. The identity of the two syncytia-forming viruses (designated MUS-WR and MUS-WL from Wild Rose Hatchery and Wolf Lake State Fish Hatchery, respectively) as strains of FHMNV was determined based on multiple-gene sequencing and phylogenetic analyses. The pathogenicity of the MUS-WL FHMNV strain was determined by experimentally infecting naive juvenile Muskellunge through intraperitoneal injection with two viral concentrations (63 and 6.3 × 103 TCID50/fish). Both doses resulted in 100% mortality in experimentally infected fish, which exhibited severely pale gills and petechial hemorrhaging in eyes, fins, and skin. Histopathological alterations in experimentally infected fish were observed mainly in the hematopoietic tissues in the form of focal areas of necrosis. Phylogenetic analysis of concatenated partial spike glycoprotein and helicase gene sequences revealed differences between the MUS-WL FHMNV, MUS-WR FHMNV, and two other FHMNV originally isolated from moribund Fathead Minnows Pimephales promelas including the index FHMNV strain (GU002364). Based on a partial helicase gene sequence, a reverse transcriptase PCR assay was developed that is specific to FHMNV. These results give evidence that the risks posed to Muskellunge by FHMNV should be taken seriously.
Received May 1, 2015; accepted February 8, 2016
‘Powerful knowledge’ curriculum theories and the case of physics
Authors: Yates Lyn , Millar Victoria 
Published: May 2016

ABSTRACTA stream of debate (including a previous special issue of this journal (25(1) 2014)) has made claims not just for ‘bringing knowledge back in’ as the framing underpinning of the school curriculum, but that subjects associated with disciplinary and disciplined knowledge forms have a particular power and that these characteristics are important to preserve in curriculum frameworks. This paper draws on a major Australian research project studying school and university physics in the context of these arguments to revisit the issue of the ‘discipline’ of physics and the curriculum logics for physics. Given that disciplines are social in origin and changing and expanding over time, can school curriculum be logically derived from the discipline to which they relate? Are questions about student engagement only questions about pedagogy and not curriculum? Does a focus on disciplinary knowledge mean that the role of school in forming identities and values is avoidable as a significant feature of what the curriculum does? The findings from the project are used both to illustrate and test these questions, and to challenge some over‐simple assumptions about the verticality of this form of knowledge for education purposes.
‘I assume they don't think!’: teachers' perceptions of Normal Technical students in Singapore
Authors: Heng Mary Anne , Atencio Matthew 
Published: May 2016

ABSTRACTTeachers' attitudes and beliefs about students in a particular grouping hierarchy are shaped by their ideological views. Within education systems that have high international profiles, such as Singapore, what has been less explored is how teachers think about and interact with students who are seemingly positioned as being ‘low‐ability’ or ‘low achieving’ within disadvantaged education contexts. This paper accordingly examines teachers' perceptions of ‘Normal Technical’ students in Singapore. The findings underscore the need to understand teachers' interpretations and thinking about teaching and learning processes that influence classroom practice. This is a necessary starting point for pedagogical innovation and change in schools.
Habitable worlds with JWST: transit spectroscopy of the TRAPPIST-1 system?
Authors: Barstow J. K. , Irwin P. G. J. 
Published: May 2016

Abstract
The recent discovery of three Earth-sized, potentially habitable planets around a nearby cool star, TRAPPIST-1, has provided three key targets for the upcoming James Webb Space Telescope (JWST). Depending on their atmospheric characteristics and precise orbit configurations, it is possible that any of the three planets may be in the liquid water habitable zone, meaning that they may be capable of supporting life. We find that present-day Earth levels of ozone, if present, would be detectable if JWST observes 60 transits for innermost planet 1b and 30 transits for 1c and 1d.
Clozapine Rechallenge After Neutropenia or Leucopenia
Authors: Prokopez Cintia R. , Armesto Arnaldo R. , Gil Aguer María F. , Balda María V. , Papale Rosa M. , Bignone Inés M. , Daray Federico M. 
Published: May 2016

Abstract
To rechallenge with clozapine for a patient who previously has experienced neutropenia or leucopenia or during clozapine treatment is a difficult clinical decision. Herein, we analyzed the results of such a rechallenge in 19 patients. We analyzed all the reports, from the database of the pharmacovigilance department of the Argentine National Administration of Drugs, Foods, and Medical Devices, of patients who were rechallenged with clozapine after a leucopenia or a neutropenia. Nineteen cases of rechallenge after leucopenia or neutropenia were reported between 1996 and 2014. One third of the patients re-exposed to clozapine developed a new hematologic adverse reaction. The second blood dyscrasia was less severe in 83% of the cases and had a shorter median latency as compared with the first (8 weeks vs 182 weeks, P = 0.0045). There were no significant differences for demographic and clinical characteristics of patients who developed a second dyscrasia as compared with those who did not. The present study shows that almost 70% of the patients rechallenged with clozapine after a leucopenia or a neutropenia did not develop a new hematological adverse effect, whereas the remaining 30% had a faster but less serious neutropenia.
Essentials of Advocacy in Case Management: Part 1
Authors: Tahan Hussein M. 
Published: May 2016

Purpose/Objectives:
This article describes the meaning and underpinnings of advocacy in the field of case management and shares essential principles and concepts for effective client advocacy.

Primary Practice Setting(s):
All practice settings across the continuum of health and human services and case managers of diverse professional backgrounds.

Findings and Conclusion:
Advocacy is vital to case management practice and a primary role of the professional case manager. It is rooted in ethical theory and principles. Successful case managers apply advocacy at every step of the case management process and in every action they take. Part I of this 2-part article explores the ethical theories and principles of advocacy, the perception of case management-related professional organizations of advocacy, and types of advocacy. Part II then presents a client advocacy model for case managers to apply in their practice, describes the role of advocacy in client engagement, and identifies important strategies and a set of essential competencies for effective case management advocacy.

Implications for Case Management:
Acquiring foundational knowledge, skills, and competencies in what advocacy is equips case managers with the ability and confidence to enact advocacy-related behaviors in the provision of care to achieve desired outcomes for both the clients and health care agencies/providers alike. Case management leaders may use the knowledge shared in this article to develop advocacy training and competency programs for their case managers.
Contrast-Enhanced Ultrasound
Authors: Denham Stephanie LeeAnn Wilson , Alexander Lauren F. , Robbin Michelle L. 
Published: May 2016

Abstract
The use of microbubble contrast greatly enhances the ability of ultrasound to delineate structures and therefore aid in diagnosis. Ultrasound microbubble contrast agents are composed of low-solubility gas encapsulated in a biomaterial shell. These agents use the physics of ultrasound imaging to effectively identify and characterize focal hepatic and renal lesions. Not only can contrast agents be used to evaluate multiple phases of lesion contrast enhancement, but ultrasound also allows for real-time study of enhancement patterns. The short half-life and intravascular location of the microbubbles allows for multiple, sequential administrations of contrast to observe enhancement of lesions in different sites. Furthermore, the ability to perform imaging without ionizing radiation and the lack of nephrotoxicity make contrast-enhanced ultrasound an ideal evaluation method for patients who need serial surveillance or in whom imaging options are severely limited because of renal insufficiency. These techniques are widely used in many countries for diagnostic radiological purposes; however, the lack of both Food and Drug Administration approval and reimbursement for noncardiac hospital-based imaging has delayed widespread use in the United States. Despite these limitations, continued research and innovations in ultrasound contrast make it essential to have a working knowledge of the typical enhancement patterns of frequently seen hepatic and renal lesions as these techniques offer an alternative option for contrast imaging.
Knowledge.Bio: A Web Application for Exploring, Building and Sharing Webs of Biomedical Relationships Mined from PubMed
Authors: Bruskiewich Richard , Huellas-Bruskiewicz Kenneth , Ahmed Farzin , Kaliyaperumal Rajaram , Thompson Mark , Schultes Erik , Hettne Kristina M. , Su Andrew I. , Good Benjamin M. 
Published: May 2016

AbstractKnowledge.Bio is a web platform that enhances access and interpretation of knowledge networks extracted from biomedical research literature. The interaction is mediated through a collaborative graphical user interface for building and evaluating maps of concepts and their relationships, alongside associated evidence. In the first release of this platform, conceptual relations are drawn from the Semantic Medline Database and the Implicitome, two compleme ntary resources derived from text mining of PubMed abstracts.Availability— Knowledge.Bio is hosted athttp://knowledge.bio/and the open source code is available athttp://bitbucket.org/sulab/kb1/.Contact—asu@scripps.edu;bgood@scripps.edu
First Lean, then modularization: improving the maintenance of offshore wind turbines
Authors: Petersen Kristian R. , Madsen Erik Skov , Bilberg Arne 
Published: May 2016

PurposeThis paper aims to explore how maintenance tasks can be planned and executed in a smarter way and, consequently, how the operations and maintenance of offshore wind power installations can be improved through modularisation.Design/methodology/approachThis is a case study of one of Europe’s leading offshore wind power operators with more than 1,000 wind turbine generators in operation. By focusing on this company, in-depth insights into its operations and maintenance processes are investigated.FindingsLean is identified to constitute an important first step before the modularisation of maintenance tasks. The modularisation of the maintenance of offshore wind farms is identified to reduce preventive maintenance times.Practical implicationsThe paper develops a process to identify the resources needed for maintenance before the modularisation of maintenance tasks and resources can take place. The authors also establish a foundation for the development of a software tool to support the development of the modularisation of maintenance tasks.Originality/valueThe present study contributes to the rather immature field of research on the operations and maintenance of offshore wind power. Furthermore, it adds to the emerging research area of service modularity.
Measurement and analysis of cutting forces on single point cutting tool in turning of MS bar with dry condition and rust-X cutting fluid
Authors: Patil Pradeep Kumar , Khandwawala A I 
Published: May 2016

Purpose
– The purpose of this paper is to measure the effect of rake angle on cutting forces on the rake face of single point cutting tool with two cutting conditions. The experimental setup has been developed to measure the cutting forces. The study aims to put forward the optimum cutting condition, which improves the product quality, surface finish, productivity and tool life.

Design/methodology/approach
– The load cell-based tool dynamometer has been developed to measure the cutting forces. The experiments have performed on the mild steel bar of hardness 60 BHN. The friction and the normal forces have measured in dry cutting condition and with rust-X cutting fluids. The cutting forces for these two cutting conditions have calculated with constant depth of cut, speed and feed with different rake angles in the range of degrees 6, 7, 8, 9, 10, 11, 12, 15 and 20.

Findings
– The experimental observations shows the variations of friction and normal forces with different cutting conditions and parameters. It shows the friction force on rake face increase and the normal force on the rake face decreases with increase the rake angle.

Research limitations/implications
– The observations has done only for mild steel of hardness 60 BHN. It can also be perform on different materials and for different cutting conditions.

Practical implications
– The experimental setup developed in this research can be used in the manufacturing industry. It can help to decide and maintain the optimum cutting conditions.

Originality/value
– The observations have been made on an experimental setup, which fulfills the actual working/cutting conditions as per the use in industries.
Process and product-performance modeling for mechanical fastening by flow drilling screws
Authors: Grujicic Mica , Snipes Jennifer , Ramaswami S 
Published: May 2016

Purpose– The purpose of this paper is to propose a computational approach to establish the effect of various flow drilling screw (FS) process and material parameters on the quality and the mechanical performance of the resulting FS joints.Design/methodology/approach– Toward that end, a sequence of three distinct computational analyses is developed. These analyses include: (a) finite-element modeling and simulations of the FS process; (b) determination of the mechanical properties of the resulting FS joints through the use of three-dimensional, continuum finite-element-based numerical simulations of various mechanical tests performed on the FS joints; and (c) determination, parameterization and validation of the constitutive relations for the simplified FS connectors, using the results obtained in (b) and the available experimental results. The availability of such connectors is mandatory in large-scale computational analyses of whole-vehicle crash or even in simulations of vehicle component manufacturing, e.g. car-body electro-coat paint-baking process. In such simulations, explicit three-dimensional representation of all FS joints is associated with a prohibitive computational cost.Findings– Virtual testing of the shell components fastened using the joint connectors validated the ability of these line elements to realistically account for the strength, ductility and toughness of the three-dimensional FS joints.Originality/value– The approach developed in the present work can be used, within an engineering-optimization procedure, to adjust the FS process and material parameters (design variables) in order to obtain a desired combination of the FS-joint mechanical properties (objective function).
The equity premium puzzle: new evidence on the optimal holding period and optimal asset allocation
Authors: Zervoudi Evanthia , Spyrou Spyros 
Published: May 2016

Purpose
– The purpose of this paper is to report new original evidence on optimal holding periods and optimal asset allocations (Benartzi and Thaler, 1995).

Design/methodology/approach
– The authors employ a number of different value functions, a recent dataset, different markets, and varying investment horizons.

Findings
– The authors report original evidence across markets and over-time, employing different value functions and varying investment horizons. The results results indicate that, during the past decades, the optimal holding period (seven months during the whole period and four/five months during crises) is not affected by the value function employed, is in accordance with the Myopic Loss Aversion hypothesis, is consistent across markets, but is sensitive to economic crises and shorter to that reported in Benartzi and Thaler (12 months). The optimal asset allocation is also different to that of Benartzi and Thaler during crises periods and/or assuming value functions with probability distortion.

Originality/value
– The paper employs a number of different value functions, with and without probability distortion; it compares investor behavior in three important international markets (USA, UK, Germany); as a further robustness test the authors use various investment horizons.
Predicting financial distress: revisiting the option-based model
Authors: Agrawal Khushbu , Maheshwari Yogesh 
Published: May 2016

Purpose
– The purpose of this paper is to assess the significance of the Merton distance-to-default (DD) in predicting defaults for a sample of listed Indian firms.

Design/methodology/approach
– The study uses a matched pair sample of defaulting and non-defaulting listed Indian firms. It employs two alternative statistical techniques, namely, logistic regression and multiple discriminant analysis.

Findings
– The option-based DD is found to be statistically significant in predicting defaults and has a significantly negative relationship with the probability of default. The DD retains its significance even after the addition of Altman’s Z-score. This further establishes its robustness as a significant predictor of default.

Originality/value
– The study re-establishes the utility of the Merton model in India using a simplified version of the Merton model that can be easily operationalized by practitioners, reasonably larger sample size and is done in a more recent period covering the post global financial crisis period. The findings could be valuable to banks, financial institutions, investors and managers.