bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined SEP and anti-PD-L1 antibody produces a synergistic antitumor effect in B16-F10 melanoma-bearing mice
Authors: Hu Zhengping , Ye Liang , Xing Yingying , Hu Jinhang , Xi Tao 
Published: January 2018

AbstractThe increased PD-L1 induces poorer prognosis in melanoma. The treatment with PD-1/PD-L1 antibodies have a low response rate. The combination immunotherapies are the encouraging drug development strategy to receive maximal therapeutic benefit. In this study, we investigated the enhanced antitumor and immunomodulatory activity of combined SEP and αPD-L1 in B16-F10 melanoma-bearing mice. The results shown that combined SEP and αPD-L1 presented significant synergistic antitumor effects, increased the frequency of CD8+ and CD4+ T cells in spleen and tumor, cytotoxic activity of CTL in spleen, and IL-2 and IFN-γ levels in splenocytes and tumor. The combination treatment also produced synergistic increase in P-ERK1/2 level in spleen. Immunohistochemistry shown that SEP induced the PD-L1 expression in melanoma tissue possibly by promoting IFN-γ excretion, which led to the synergistic anti-tumor effects of aPD-L1 and SEP. Furthermore, in the purified T lymphocyte from the naive mice, the combination of SEP and αPD-L1 had more potent than SEP or αPD-L1 in promoting T lymphocyte proliferation and cytokines secretion including IL-2 and IFN-γ, at least partially by activating MEK/ERK pathway. Our study provides the scientific basis for a clinical trial that would involve combination of anti-PD-L1 mAb and SEP for sustained melanoma control.
Determining direct binders of the Androgen Receptor using a high-throughput Cellular Thermal Shift Assay
Authors: Shaw Joseph , Leveridge Mathew , Norling Charlotta , Karén Jakob , Molina Daniel Martinez , O’Neill Daniel , Dowling James E. , Davey Paul , Cowan Suzanna , Dabrowski Michael , Main Martin , Gianni Davide 
Published: January 2018

AbstractAndrogen Receptor (AR) is a key driver in prostate cancer. Direct targeting of AR has valuable therapeutic potential. However, the lack of disease relevant cellular methodologies capable of discriminating between inhibitors that directly bind AR and those that instead act on AR co-regulators has made identification of novel antagonists challenging. The Cellular Thermal Shift Assay (CETSA) is a technology enabling confirmation of direct target engagement with label-free, endogenous protein in living cells. We report the development of the first high-throughput CETSA assay (CETSA HT) to identify direct AR binders in a prostate cancer cell line endogenously expressing AR. Using this approach, we screened a pharmacology library containing both compounds reported to directly engage AR, and compounds expected to target AR co-regulators. Our results show that CETSA HT exclusively identifies direct AR binders, differentiating them from co-regulator inhibitors where other cellular assays measuring functional responses cannot. Using this CETSA HT approach we can derive apparent binding affinities for a range of AR antagonists, which represent an intracellular measure of antagonist-receptor Ki performed for the first time in a label-free, disease-relevant context. These results highlight the potential of CETSA HT to improve the success rates for novel therapeutic interventions directly targeting AR.
Speech Prosodies of Different Emotional Categories Activate Different Brain Regions in Adult Cortex: an fNIRS Study
Authors: Zhang Dandan , Zhou Yu , Yuan Jiajin 
Published: January 2018

AbstractEmotional expressions of others embedded in speech prosodies are important for social interactions. This study used functional near-infrared spectroscopy to investigate how speech prosodies of different emotional categories are processed in the cortex. The results demonstrated several cerebral areas critical for emotional prosody processing. We confirmed that the superior temporal cortex, especially the right middle and posterior parts of superior temporal gyrus (BA 22/42), primarily works to discriminate between emotional and neutral prosodies. Furthermore, the results suggested that categorization of emotions occurs within a high-level brain region–the frontal cortex, since the brain activation patterns were distinct when positive (happy) were contrasted to negative (fearful and angry) prosody in the left middle part of inferior frontal gyrus (BA 45) and the frontal eye field (BA8), and when angry were contrasted to neutral prosody in bilateral orbital frontal regions (BA 10/11). These findings verified and extended previous fMRI findings in adult brain and also provided a “developed version” of brain activation for our following neonatal study.
Compact CMOS LiT VCO achieving 198.6 dBc/Hz FoMA
Authors: Kim S.‐J. , Seo D.‐I. , Kim J.‐S. , Song R. , Kim B.‐S. 
Published: January 2018

A compact CMOS linear transconductance (LiT) voltage‐controlled oscillator (VCO) in 65 nm CMOS process is presented. The proposed LiT technique is realised using NP core without a choke inductor to reduce die area and avoid dual resonance problem. The measured output frequency of the proposed VCO shows 11.18–11.98 GHz and phase noise is −112.62 dBc/Hz at 1 MHz offset frequency. DC power consumption of the VCO core and the buffer is 5.86 and 6 mW, respectively. It achieves a high figure of merit normalised to the area of 198.6 dBc/Hz.
Multiple‐port reader antenna with three modes for UHF RFID applications
Authors: Zheng Jingming , Yang Yang , He Xiaoxiang , Mao Chenyang , Gao Jin , Zhou Chun 
Published: January 2018

A multiple‐port reader antenna for ultra‐high frequency (UHF) radio‐frequency identification (RFID) near‐field and far‐field applications which has three modes is proposed and studied. Three fed‐ports are designed into the structure, which provide different radiation properties. Specially, far‐field radiation for two orthogonal polarisations and near‐field operation based on magnetic field coupling can be modified by feeding different corresponding port. The RFID reader antenna can work at three different operations within 820–880 MHz, including European UHF (865–868 MHz) and Chinese UHF (840–845 MHz) bands. Design details, simulation, and measured results are also presented.
High‐efficiency self‐powered oscillator for capacitive neural stimulation
Authors: Hong G. , Han G. 
Published: January 2018

Implantable neural stimulation devices have drawn attention for various clinical purposes. Capacitor‐coupled stimulation inherently maintains charge‐balance, allowing the use of less complex pulse‐generating circuits. This Letter proposes a self‐powered relaxation oscillator, which allows direct charge transfer from the energy harvester to the capacitive stimulation electrode. As the proposed circuit does not require power supply to operate the oscillator itself, it achieves high power efficiency by eliminating wasted current from the power supply to the ground. A prototype of the proposed oscillator was fabricated with a 0.35 μm standard CMOS process. The prototype chip demonstrates power efficiency of up to 94.1% at 520 Hz for 1.4 V signal swing.
Enhanced morphological stability of silver nanoparticles supported on rough substrates at high temperatures
Authors: Scherzer Ryan D. , Gasparov Lev V. , Stagon Stephen P. 
Published: January 2018

Noble metal nanostructures serve as excellent substrates for the detection of trace amounts of substances in surface enhanced Raman spectroscopy, but they have not found widespread commercial use due to their instability and short shelf lives. This work demonstrates that silver nanoparticles grown onto substrates with nanoscale roughness, using glancing angle physical vapour deposition and subsequently thermally annealed at 500°C to generate a stable lower energy configuration, exhibit Raman enhancements that are strong and remain stable with no drop over 30 days. These nanoparticle arrays may serve as time stable substrates for commercial scale Raman spectroscopy or may be employed in harsh environments.
Perfection of leakage and ferroelectric properties of Ni‐doped BiFeO3 thin films
Authors: Wang Lingxu , Yang Shiju , Zhang Fengqing , Fan Suhua 
Published: January 2018

BiFe1−x Nix O3 (x = 0%, 1, 2 and 3%) films were deposited on ITO/glass substrate using sol–gel process. The work reports the impacts of Ni doped on the crystal microstructure, leakage current, conduction mechanism and ferroelectric behaviour systematically. From the XRD analysis, all samples match well with the perovskite structure without impurity phase. Polarisation‐electric filed hysteresis loop demonstrates that the optimal Ni‐doped content of BiFeO3 films is x = 2%, of which the remnant double polarisation (2Pr) is 141.4 μC/cm2 at the test electric field of 1067 kV/cm. Leakage current density curves show that Ni doping has a great contribution in reducing leakage. The value of leakage is 4.79 × 10−7 A/cm2 at tested electric field of 300 kV/cm. In addition, the leakage conduction mechanism transforms from the Ohmic conduction under the low electric field into the space charge limited conduction under high electric field. Ni doped does not cause significant change in the conduction mechanism.
Facile preparation of Ag nanocubes at 30°C under light irradiation
Authors: Li Danli , Shan Yangyang , Liu Ruiyi , Wu Qian , Zheng Tingting , Li Kai 
Published: January 2018

Preparation of silver nanocubes (Ag NCs) with low temperature and controlled shapes is challenging in an aqueous solution because of its anticipated low embodied energy and experiment controllability. In this work, Ag NCs have been successfully synthesised under facile reaction conditions (at 30°C oil bath and under daylight illumination) by coordination effects of thermal reduction and photoinduced reduction. The obtained Ag NCs were characterised by transmission electron microscopy, UV–visible spectrophotometer and X‐ray diffractometer. It turned out that, Ag NCs were prepared by using trifluoroacetic acid silver, cetyltrimethyl ammonium chloride, ascorbic acid, ferric chloride and sodium hydrosulphide as raw materials, and their sizes around 35 nm. Besides, as‐synthesised Ag NCs with uniform size and high yield would serve as outstanding substrates for surface‐enhanced Raman scattering detection, and as sacrificial templates to fabricate Au nanocages.
A new compact young moving group around V1062 Scorpii
Authors: Röser Siegfried , Schilbach Elena , Goldman Bertrand , Henning Thomas , Moor Attila , Derekas Aliz 
Published: January 2018

Aims.We are searching for new open clusters or moving groups in the solar neighbourhood.Methods.We used theGaia-TGAS catalogue, cut it into narrow proper motion and parallax slices and searched for significant spatial over-densities of stars in each slice. We then examined stars forming over-densities in optical and near-infrared colour-magnitude diagrams to determine if they are compatible with isochrones of a cluster.Results.We detected a hitherto unknown moving group or cluster in the Upper Centaurus Lupus (UCL) section of the Scorpius-Centaurus OB association (Sco-Cen) at a distance of 175 pc from the Sun. It is a group of 63 comoving stars of less than 10 to about 25 Myr in age. For the brightest stars that are present in theGaia-TGAS catalogue, the mean difference between kinematic and trigonometric distance moduli is − 0.01 mag with a standard deviation of 0.11 mag. Fainter cluster candidates are found in the HSOY catalogue, where no trigonometric parallaxes are available. For a subset of our candidate stars, we obtained radial velocity measurements at the MPG/ESO 2.2 m telescope in La Silla. Altogether we found 12 members with confirmed radial velocities and parallaxes, 31 with parallaxes or radial velocities, and 20 candidates from the convergent point method. The isochrone masses of our 63 members range from 2.6 to 0.7M⊙.
Preoperative FIBTEM Predicts Postoperative Hemorrhage in Total Knee Arthroplasty
Authors: Kim Seong-Hyop , Oh Chung-Sik , Kim Tae-Hoon , Park Sewon , Rhee Ka Young 
Published: January 2018

AbstractFIBTEM parameters might predict the amount of postoperative hemorrhage following total knee arthroplasty (TKA), because fibrin polymerization and fibrinolysis have a central role in postoperative hemorrhage following TKA. This study retrospectively evaluated 54 patients who had undergone unilateral primary TKA. Laboratory coagulation parameters, including FIBTEM, were recorded before anesthesia induction and after admission to the postanesthetic care unit. The decrease in hemoglobin (Hb), amount of hemorrhage via closed suction drainage, fluid administration, and amount transfused were reviewed postoperatively. The preoperative FIBTEM amplitudes 10 (A10) and 20 (A20) minutes after clotting time and maximum clot firmness (MCF) had the highest correlations with the postoperative decrease in Hb (p = 0.001, p = 0.002, and p = 0.003, respectively). The preoperative FIBTEM A10 <19 mm, A20 <20 mm, and MCF < 21 mm predicted > 3.0 g/dL postoperative Hb decrement from the postanesthetic Hb value (p = 0.004, p = 0.007, and p = 0.012, respectively). Preoperative FIBTEM can predict the amount of postoperative hemorrhage following TKA.
A Statewide Medication Management System: Health Information Exchange to Support Drug Therapy Optimization by Pharmacists across the Continuum of Care
Authors: Pellegrin Karen , Chan Francis , Pagoria Natalie , Jolson-Oakes Sheena , Uyeno Reece , Levin Andrew 
Published: January 2018

Background While evidence generally supports the use of medication management technology, systems are typically implemented and evaluated piecemeal rather than as part of a comprehensive model for medication management. Systems to support drug therapy optimization, increasingly a key role of pharmacists in our healthcare system, have not yet been reported.
Objective Our objective is to describe the design, implementation, and use of health information technology to support the hospital and community pharmacists' management of medications for high-risk patients statewide in the “Pharm2Pharm” model of care. Our aims were to make it easier for the pharmacists to access information needed to identify and resolve drug therapy problems using best practices for medication management and communicate with other members of the care team.
Methods The pharmacist's roles and the medication management processes guided the design of the supporting technology, which was implemented after the Pharm2Pharm model was launched and the pharmacists' technology needs were assessed. Priorities for technology included sending care transition documents from hospital to community pharmacist securely and efficiently, access to medical records, including medications and laboratory results, documentation, and patient tracking. Implementation and use of the technology were documented.
Results Communications, medication management, and population management solutions were implemented to support the Pharm2Pharm model. The pharmacists delivering services through this model adopted and meaningfully used this technology to support their work.
Conclusion Implementing technology with value outside of the Pharm2Pharm model was a strategic approach to investment. This work emphasizes the importance of shifting the focus of technology from supporting a specific piece of the medication management process to supporting the goal of optimizing medication regimens. Health information exchange systems can provide important technology needed to integrate pharmacists into care teams as they are deployed to improve patient outcomes.
Low Vitamin D Blood Levels with Significant Differences Between the Sexes in Viennese School Children – the EDDY Study
Authors: Mehany Sarah , Pöppelmeyer Christina , Helk Oliver , Pachinger Otmar , Widhalm Kurt 
Published: January 2018

Zusammenfassung
Hintergrund Die Bestimmung des Serumspiegels von 25(OH)-Vitamin D wird für die Erfassung des Vitamin-D-Status einer Person empfohlen. Vitamin-D-Mangel ist weit verbreitet unter europäischen Jugendlichen. Die Bedeutung von Vitamin D für die Mineralisierung der Knochen und für die Risikoreduktion von Atemwegsinfekten ist gesichert.
Material und Methodik Im Rahmen der EDDY-Studie wurden 25(OH) Vitamin-D-Spiegel bei 138 Kindern (47,8 % weiblich) im Alter von 11 – 13 Jahren in 4 verschiedenen Wiener Schulen bestimmt. Drei Blutabnahmen wurden zu verschiedenen Jahreszeiten durchgeführt. 25(OH)-Vitamin D wurde im Serum mittels CLIA (Chemiluminescent Immunoassay) bestimmt. Dieselben Vitamin-D-Kategorien wie in der HELENA-Studie wurden verwendet.
Ergebnisse Im September und Oktober 2013 hatten lediglich 36,9 % der Kinder 25(OH)-Vitamin-D-Spiegel > 50 nmol/l (untere Grenze für eine adäquate Vitamin-D-Versorgung) und nur 9,4 % wiesen Werte ≥ 75 nmol/l auf. Von den Kindern mit unzureichender Versorgung wiesen 20,3 % 25(OH)-Vitamin-D-Spiegel < 27,5 nmol/l auf. Es bestand ein signifikanter Unterschied in mittleren Vitamin-D-Spiegeln zwischen Mädchen und Jungen (♀ 40,9 ± 20,2 nmol/l versus ♂ 49,9 ± 22,1 nmol/l, p = 0,015), welcher sich auch in den Ergebnissen der zweiten und dritten Blutabnahme präsentierte. In den Wintermonaten hatten nur 0,9 % der Kinder Vitamin-D-Werte > 75 nmol/l.
Schlussfolgerung Vitamin-D-Mangel scheint unter Wiener Jugendlichen im Alter zwischen 11 und 13 Jahren weit verbreitet zu sein. 9,4 % der Kinder hatten im Herbst „optimale“ 25(OH)-Vitamin-D-Spiegel ≥ 75 nmol/l, in den Wintermonaten nur noch 0,9 %.
Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release
Authors: Hoernes Thomas Philipp , Clementi Nina , Juen Michael Andreas , Shi Xinying , Faserl Klaus , Willi Jessica , Gasser Catherina , Kreutz Christoph , Joseph Simpson , Lindner Herbert , Hüttenhofer Alexander , Erlacher Matthias David 
Published: January 2018

Significance
Translation termination is a crucial process during protein synthesis. Class I release factors (RFs) are in charge of recognizing stop codons and consequently hydrolyzing the peptidyl-tRNA at the ribosomal P site. High-resolution crystal- and cryo-EM structures of RFs bound to the ribosome revealed a network of potential interactions that is formed between the mRNA and RFs; however, it remained enigmatic which interactions are critical for accurate stop codon recognition and peptide release. By using chemically modified stop codon nucleotides, the importance and the contribution of single hydrogen bonds to stop codon recognition was investigated. This approach revealed a detailed picture of chemical groups defining a stop codon and contributing to the discrimination against sense codons during prokaryotic and eukaryotic translation termination.
Identification of Polygonum orientale constituents using high-performance liquid chromatography high-resolution tandem mass spectrometry
Authors: Shin Hyeji , Park Yeeun , Jeon Young Ho , Yan Xi-Tao , Lee Ki Yong 
Published: January 2018

Abstract
Our primary focus in this research was to identify and characterize its bioactive compounds for potential therapeutic use. Twenty-seven metabolites of Polygonum orientale were identified using LC-QTOF tandem mass spectrometry. Interestingly, P. orientale extracts included several highly oxygenated flavonoids were isolated from P. orientale by column chromatography. 13C NMR data of highly oxygenated flavonoids (1–7) are reported here for the first time. In addition, nitric oxide, 1,1-diphenyl-2-picrylhydrazyl, and water-soluble tetrazolium salt assays were carried out on the isolated compounds to investigate their anti-inflammatory, anti-oxidant, and neuroprotective activities, respectively. Compounds 1, 2, 3, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells without affecting cell viability. Compounds 9–12 exhibited significant antioxidant activity, while compounds 8, 9, and 12 exhibited protective effects against glutamate-induced neurotoxicity in HT22 cells. Our results indicate that P. orientale is a promising source of natural agents for the potential treatment of inflammation and neurodegenerative diseases.
Novel tyrosinase inhibitors from liquid culture ofNeolentinus lepideus
Authors: Ishihara Atsushi , Ide Yuri , Bito Tomohiro , Ube Naoki , Endo Naoki , Sotome Kozue , Maekawa Nitaro , Ueno Kotomi , Nakagiri Akira 
Published: January 2018

AbstractTyrosinase is the key enzyme that controls melanin formation in the human skin. We performed a screening of 96 extracts of mushroom cultures and fruiting bodies for examining their inhibitory activity against mushroom tyrosinase. The ethyl acetate extracts of culture filtrate of Neolentinus lepideus exhibited the strongest inhibitory activity. The active compounds 1 and 2 were purified by repeated chromatographic separations from the extract. On the basis of spectroscopic analyses, 1 and 2 were identified to be 1,3-dihydroisobenzofuran-4,5,7-triol and 5-methoxy-1,3-dihydroisobenzofuran-4,7-diol, respectively. Lineweaver-Burk plot of the enzyme reaction in the presence of 1 indicated that 1 was a potent competitive inhibitor. The respective IC50 values of 1 and 2 were 173 and 263 μg/mL. Compound 1 at 15 μg/mL suppressed melanin accumulation stimulated by α-MSH in the murine melanoma B16 cells, as well as the induced accumulation of both tyrosinase transcript and protein without inhibiting cell proliferation.
Cash transfers for families and children in Argentina, Brazil and Chile: segmented expansion or universal benefits?
Authors: Arza Camila 
Published: January 2018

AbstractOver the past two decades, most Latin American countries have developed cash transfer programmes for poor and low income families not covered by the social security system. These programmes now benefit about 127 million people throughout Latin America and in some countries they include more children than the family allowances of the social insurance system. How have these innovations changed the structure of child benefits in Latin America? This article discusses this issue by looking at the experiences of Argentina, Brazil, and Chile. In these three countries, a wide-ranging programme of non-contributory cash transfers for low income families now operates alongside a pre-existing scheme of family allowances for formal sector workers. This has contributed to expand coverage of cash benefits for families and children, while at the same time keeping a segmented benefit structure. In contrast, a universal child benefit could overcome this segmentation and guarantee equal rights to all children.