bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Knockdown of Kv4.1 Regulates Repetitive Firing Rates and Clock Gene Expression in the Suprachiasmatic Nucleus and Daily Rhythms in Locomotor Behavior
Authors: Hermanstyne Tracey O. , Granados-Fuentes Daniel , Mellor Rebecca L. , Herzog Erik D. , Nerbonne Jeanne M. 
Published: May 2017

AbstractRapidly activating and inactivating A-type K+currents (IA) encoded by Kv4.2 and Kv4.3 pore-forming (α) subunits of the Kv4 subfamily are key regulators of neuronal excitability. Previous studies have suggested a role for Kv4.1 α-subunits in regulating the firing properties of mouse suprachiasmatic nucleus (SCN) neurons. To test this, we utilized an RNA-interference strategy to knockdown Kv4.1, acutely and selectively, in the SCN. Current-clamp recordings revealed that thein vivoknockdown of Kv4.1 significantly (p< 0.0001) increased mean ± SEM repetitive firing rates in SCN neurons during the day (6.4 ± 0.5 Hz) and at night (4.3 ± 0.6 Hz), compared with nontargeted shRNA-expressing SCN neurons (day: 3.1 ± 0.5 Hz; night: 1.6 ± 0.3 Hz). IAwas also significantly (p< 0.05) reduced in Kv4.1-targeted shRNA-expressing SCN neurons (day: 80.3 ± 11.8 pA/pF; night: 55.3 ± 7.7 pA/pF), compared with nontargeted shRNA-expressing (day: 121.7 ± 10.2 pA/pF; night: 120.6 ± 16.5 pA/pF) SCN neurons. The magnitude of the effect of Kv4.1-targeted shRNA expression on firing rates and IAwas larger at night. In addition, Kv4.1-targeted shRNA expression significantly (p< 0.001) increased mean ± SEM nighttime input resistance (Rin; 2256 ± 166 MΩ), compared to nontargeted shRNA-expressing SCN neurons (1143 ± 93 MΩ). Additional experiments revealed that acute knockdown of Kv4.1 significantly (p< 0.01) shortened, by ∼0.5 h, the circadian period of spontaneous electrical activity, clock gene expression and locomotor activity demonstrating a physiological role for Kv4.1-encoded IAchannels in regulating circadian rhythms in neuronal excitability and behavior.
Pasireotide in an insulin-requiring diabetic acromegalic patient without worsening of hyperglycemia
Authors: Gordon Murray B , Spiller Kellie L 
Published: May 2017

Summary
Long-acting pasireotide is an effective treatment option for acromegaly, but it is associated with hyperglycemia, which could impact its use in patients with diabetes. We present a case of a 53-year-old man with acromegaly and type 2 diabetes mellitus (glycated hemoglobin (HbA1c): 7.5%), who refused surgery to remove a pituitary macroadenoma and enrolled in a Phase 3 clinical trial comparing long-acting pasireotide and long-acting octreotide in acromegalic patients. The patient initially received octreotide, but insulin-like growth factor 1 (IGF-1) levels remained elevated after 12 months (383.9 ng/mL; 193.0 ng/mL; reference range: 86.5–223.8 ng/mL), indicating uncontrolled acromegaly. He switched to pasireotide 40 mg and subsequently increased to 60 mg. Within 6 months, IGF-1 levels normalized (193.0 ng/mL), and they were mostly normal for the next 62 months of treatment with pasireotide (median IGF-1: 190.7 ng/mL). Additionally, HbA1c levels remained similar to or lower than baseline levels (range, 6.7% to 7.8%) during treatment with pasireotide despite major changes to the patient’s antidiabetic regimen, which included insulin and metformin. Uncontrolled acromegaly can result in hyperglycemia due to an increase in insulin resistance. Despite having insulin-requiring type 2 diabetes, the patient presented here did not experience a long-term increase in HbA1c levels upon initiating pasireotide, likely because long-term control of acromegaly resulted in increased insulin sensitivity. This case highlights the utility of long-acting pasireotide to treat acromegaly in patients whose levels were uncontrolled after long-acting octreotide and who manage diabetes with insulin.

Learning points

Long-acting pasireotide provided adequate, long-term biochemical control of acromegaly in a patient with insulin-requiring type 2 diabetes mellitus who was unresponsive to long-acting octreotide.

Glycemic levels initially increased after starting treatment with pasireotide but quickly stabilized as acromegaly became controlled.

Long-acting pasireotide, along with an appropriate antidiabetic regimen, may be a suitable therapy for patients with acromegaly who also have insulin-requiring type 2 diabetes mellitus.
Metabolic Adaptation to Nutrients Involves Coregulation of Gene Expression by the RNA Helicase Dbp2 and the Cyc8 Corepressor in Saccharomyces cerevisiae
Authors: Wang Siwen , Xing Zheng , Pascuzzi Pete E , Tran Elizabeth J 
Published: May 2017

Abstract
Cells fine-tune their metabolic programs according to nutrient availability in order to maintain homeostasis. This is achieved largely through integrating signaling pathways and the gene expression program, allowing cells to adapt to nutritional change. Dbp2, a member of the DEAD-box RNA helicase family in Saccharomyces cerevisiae, has been proposed to integrate gene expression with cellular metabolism. Prior work from our laboratory has reported the necessity of DBP2 in proper gene expression, particularly for genes involved in glucose-dependent regulation. Here, by comparing differentially expressed genes in dbp2∆ to those of 700 other deletion strains from other studies, we find that CYC8 and TUP1, which form a complex and inhibit transcription of numerous genes, corepress a common set of genes with DBP2. Gene ontology (GO) annotations reveal that these corepressed genes are related to cellular metabolism, including respiration, gluconeogenesis, and alternative carbon-source utilization genes. Consistent with a direct role in metabolic gene regulation, loss of either DBP2 or CYC8 results in increased cellular respiration rates. Furthermore, we find that corepressed genes have a propensity to be associated with overlapping long noncoding RNAs and that upregulation of these genes in the absence of DBP2 correlates with decreased binding of Cyc8 to these gene promoters. Taken together, this suggests that Dbp2 integrates nutrient availability with energy homeostasis by maintaining repression of glucose-repressed, Cyc8-targeted genes across the genome.
Reemplazo total de rodilla en secuela de fracturas alrededor de la rodilla
Authors: Gutierrez Olivera Natalia , Salcedo Zunino Leandro Ariel , Pioli Ignacio , Bidolegui Fernando , Vindver Gabriel , Allende Bartolome Luis 
Published: May 2017

<p><strong>Introducción</strong></p><p>El reemplazo total de rodilla en secuela de trauma, secundaria a fracturas periarticulares de fémur distal o tibia proximal, implica un tratamiento complejo pero es una opción válida para el manejo de estos pacientes. El ojetivo de este trabajo es evaluar en forma retrospectiva los resultados obtenidos en RTR en pacientes con secuela de fracturas peri articulares de la rodilla.</p><p><strong>Material y Métodos</strong></p><p><strong></strong>En un estudio retrospectivo, multicéntrico donde se evaluaron 29 pacientes, con artrosis secundaria a fracturas a los que se les realizó RTR entre 2008-2015. 19 mujeres y 10 varones. Edad promedio 59 años. 23 fracturas en tibia proximal y 6 en fémur distal: 6 extraarticulares y 23 intraarticulares. Tratamiento inicial: ortopédico en 8 pacientes; reducción abierta y fijación interna (RAFI) en 21. Promedio de cirugías hasta la artroplastia 2,14 (1 a 7). Se empleó el Knee Society Score (KSS) y radiografías para la evaluación pre y postquirúrgica. Rango de movimiento (ROM) prequirúrgico: flexión 60° (45°-90°), extensión 15° (0°-30°). KSS dolor 30 (10-45) y para KSS funcional 25 (20-45). KSS total 30 (20-60)</p><p><strong>Resultados</strong></p><p><strong></strong>El seguimiento promedio 45 meses (6-104). Se restauró el eje mecánico en todos los casos. ROM en el último control: flexión 112° (60°-140°); extensión 5° (0°-30°). KSS dolor 40 (30-50) y KSS funcional 35 (30-50). KSS total 81 (50-95) Resultados buenos (KSS ≥80 puntos) 22 pacientes (75,8%), regulares 6 (20,6%) y pobres 1 caso (3,44 %).. 13% de complicaciones mayores.</p><p><strong>Discusión</strong></p><p><strong></strong>Si bien el RTR para el tratamiento de secuelas de fracturas periarticulares de la rodilla, presenta mayor porcentaje de complicaciones, siendo los resultados inferiores a los publicados en RTR en artrosis no traumaticade rodilla, el RTR es una opcion valida para el rescate postraumatico, obteniendo una mejoria significativa tanto en el KSS como en el rango de movimiento. El mismo implica un manejo de partes blandas minucioso, y una mayor complejidad técnica.</p>
Colgajo fasciocutáneo sural para la cobertura del tercio distal de pierna y pie. [Fasciocutaneous sural flap for softtissue coverage in the third distal leg, ankle and foot.]
Authors: Carabelli Guido , Barla Jorge Daniel , Taype Danilo Roger , Sancineto Carlos Federico 
Published: May 2017

<p><strong>Introducción</strong></p><p><strong></strong>Los traumatismos de alta energía en miembros inferiores se asocian con frecuencia a defectos de partes blandas, y su reconstrucción puede presentarse como una tarea desafiante, siendo en el extremo dista de la pierna y el pie los colgajo de perforantes la mejor opción de cobertura.</p><p>El motivo de este trabajo es presentar el resultado del tratamiento de lesiones de partes blandas de pierna distal, tobillo y talón utilizando exclusivamente el colgajo de perfotantes fasciocutáneo sural.</p><p><strong>Material y métodos</strong></p><p><strong></strong>Entre marzo de 2008 y febrero de 2016 se realizaron en nuestro hospital 37 colgajos fasciocutáneos surales en 35 pacientes. El criterio de inclusión fue todo paciente con defecto tegumentario en tercio distal de tibia y talón con exposición ósea, tendinosa o defecto de la almohadilla plantar.</p><p>La edad promedio fue de 49,6 años (8-90) con un seguimiento promedio de 18 meses (1-60 meses).</p><p><strong>Resultados</strong></p><p><strong></strong>De los 37 colgajos realizados 29, incluyendo a aquellos realizados en dos tiempos, no tuvieron complicaciones con buena adaptación local y sin necrosis, 3 presentaron necrosis parcial que fue mejorada con el avance del colgajo o injerto de piel parcial sin mayor necesidad de procedimientos y 5 colgajos presentaron necrosis completa. El tamaño del defecto fue de 9,6 x 6,7 (15 x 9) cm.</p><p><strong>Conclusión</strong></p><p><strong></strong>Aunque nuestra serie no es extensa, nuestros resultados son coincidentes con la literatura. Creemos que este colgajo es una alternativa viable para lesiones distales de pierna y pie con un bajo porcentaje de complicaciones y morbilidad en el miembro afectado, y con resultados satisfactorios.</p>
Sacrectomía parcial por abordaje posterior único. [Partial sacrectomy by single posterior approach.]
Authors: Bazán Pedro Luis , Terraza Sergio , Borri Álvaro Enrique , Medina Martin 
Published: May 2017

<p class="TRABAJOS"><strong>Introducción: </strong>Los tumores del sacro corresponden a menos del 7% de los tumores espinales. Prevalecen los secundarios por mieloma múltiple o carcinomas de próstata, mama, pulmón o colón. De los primarios, el cordoma es el maligno más frecuente y el tumor de células gigantes el benigno.</p><p class="TRABAJOS">Por su evolución, compromiso de estructuras extra óseas y la escasa respuesta a la coadyuvancia, la cirugía es el tratamiento más utilizado, la vía de abordaje y la necesidad de instrumentación dependerá del tumor a tratar.</p><p class="TRABAJOS"><strong>Objetivos:</strong> Los objetivos de este trabajo son: evaluar la utilización de resección parcial de sacro, analizar la técnica quirúrgica y reconocer complicaciones.</p><p class="TRABAJOS"><strong>Material y métodos:</strong> Cuatro pacientes con diagnóstico de tumor sacro ubicado por debajo de S1, que consulta por dolor y que las imágenes confirman la lesión. A todos se les realiza una resección en bloque por vía posterior preservando S1. Se describe la técnica.</p><p class="TRABAJOS"><strong>Resultados:</strong> La anatomopatológica fue: un cordoma, un tumor maligno de vaina nerviosa, un condrosarcoma y una metástasis de carcinoma prostático. Se preservo la función de S1 en todos los pacientes; uno presenta disfunción vesical permanente. Se observaron una dehiscencia de herida, una infección y fistula de líquido cefalorraquídeo. Con un seguimiento entre 6 y 24 meses todos los pacientes se encuentran libre de patología.</p><p class="TRABAJOS"><strong>Conclusiones: </strong>La resección parcial del sacro por abordaje único vía posterior puede ser indicada cuando la lesión comprometa desde S2 hacia distal y sin compromiso sacroilíaco. La preservación de raíces es de vital importancia para garantizar mejores resultados post operatorios y menor tasa de infección.</p>
Increase in resistance of pigs to oxidative stress by means of plant origin
Authors: Zhukova I. O. , Molchanov A. A. , Antipin S. L. 
Published: May 2017

Pig breeding in Ukraine is a priority and perspective branch of agriculture to solve the problem of providing the population with the food products of high quality (meat, fat). Meat production is one of the most complicated industries in the field of agriculture. The problem of providing the population with meat is almost impossible to solve without the intensive development of the industry since pigs are different from other farm animals by the premature and high output of slaughter products. The article presents the data of the research on the state of the antioxidant system of pigs when using Macleay and the additives of raw buckwheat «buckwheat green» as the source of quercetin to the feeds. As a result of the experiment it has been found out that the addition of Macleay to the feeds of pigs at the dose of 72 and 210 mg/10 kg of feeds as well as «green buckwheat» at the rate of 1.25 and 6.25 g/kg of body weight in all groups led to the increase in the weight gain of piglets on average by 10–40% and it stimulated the processes of antioxidant protection in the experimental piglets that was characterized by the inhibition of the processes of peroxidation of lipids (POL) (the decrease in the concentration of its primary and secondary TBA-active products– dien conjugates (DC) and malonic dialdehyde (MDA)) and also at first the increase and then the decrease to the normal physiological activity of antioxidant enzymes (catalase and superoxide dismutase (SOD)) and the restoration of the pool of endogenous total antioxidant activity (AOA).
Genetic polymorphism of the Landrace pig based on microsatellite markers
Authors: Lugovoy S. I. , Kramarenko S. S. , Lykhach V. Ya. 
Published: May 2017

The aim of this study was to analyze the genetic variability and population structure of the Landrace population by using 12 microsatellite markers. A total of 90 pigs representing one commercial breed (Landrace) were sampled. Twelve microsatellite loci (SW24, S0155, SW72, SW951, S0386, S0355, SW240, SW857, S0101, SW936 SW911 and S0228) were selected and belong to the list of microsatellite markers recommended by FAO/ISAG. GenAIEx software was used to calculate the allele frequencies, effective number of alleles (Ae), observed (Ho) and expected (He) heterozygosity, within-population inbreeding estimate (Fis), Shannon’s information index (ISh). Overall allele frequency values ranged from 0.006 to 0.9333 (at allele SW951120). The number of observed alleles (Na) detected ranged from 5 (S0155 and SW911) to 13 (SW72), with an overall mean of 9.00 ± 0.80 and a total of 108 alleles were observed at these loci. However, the effective number of alleles (Ae) ranged from 1.57 (SW951) to 5.49 (SW240) with a mean of 3.29 ± 0.33. Shannon’s information index (ISh) which measures the level of diversity, was sufficiently high – from 0.79 (for SW951) to 2.01 (for SW240) – with a mean of 1.43 ± 0.09. The overall means for observed (Ho) and expected (He) heterozygosities were 0.578 ± 0.009 and 0.662 ± 0.004, respectively, which ranged from 0.307 (SW951) to 0.814 (SW857) and 0.361 (SW951) to 0.818 (SW240), respectively. Of the 12 microsatellites analyzed using Fisher’s exact test, 50% were in Hardy-Weinberg equilibrium, and 6 were out of equilibrium (P < 0.05). Three mutation models namely, infinite allele model (I.A.M.), two phase model (T.P.M.), stepwise mutation model (S.M.M.) were estimated using the BOTTLENECK software. The results are indicated that the Landrace pig population is non-bottlenecked and remained at mutation-drift equilibrium. The study stands first in genetic characterization of the Ukrainian Landrace pig population through microsatellite markers. The various parameters and values used to quantify genetic variability, such as the high mean (and effective) number of alleles and the expected and observed heterozygosities, indicated high genetic variability in the Ukrainian Landrace pigs. The population has not undergone any recent and/or sudden reduction in the effective population size and remained at mutation-drift equilibrium.
Impact complexes nanokarboksylativ and drug Kvatronan-Se on hematological parameters of blood of experimental animals
Authors: Seba N. B. , Khomenko M. A. 
Published: May 2017

In this paper present the results of research of influence of the preparation «Kvatronan-Se» and carboxylates of Se, Cu, Mn, Cr, Ge in different combinations on haematological parameters of blood of experimental animals. Study was carried out on the basis of PC Haleks-Agro. It is located in the village Gul'sk of Novograd Volynskiy district of Zhytomyr region. In the farm to bred of Simmental cows. For the experiment was formed four groups. We took into account age, live weight of 650–700 kg and the average milk yield 5000–6000 kg. Animals is located in identical conditions of feeding and maintenance. Research experiment was carried out on animals couples counterparts. Preparations introduced animals three times at 10–12 days after insemination. The cows first experimental group were injected complex nanokarboksylativ Ge, Cu, Mn and Cr, second – drug Kvatronan–Se and animals third experimental group was administered a complex composed of Se, Cu, Mn, Cr. On 13 day after insemination in experimental animals were taken blood from tail vein and examined the contents of hematological parameters. Established that injection nanokarboksylativ enhance the fertility of cows in the first and third group, the figure rose to 20% in the second by 40% compared with the control. Research hematological parameters blood animals shows that after entering content drugs of blood cells had minor changes but remained within the physiological norm. The results show that in concentration monocyte the second group was at 5.53% (P > 0.01) higher compared to the control group and 2.3% compared to the first and third. The content of lymphocytes in this group decreased by 26.3% (P > 0.05), 22% and 16% relative to the control, the first and third groups. Also, it should be noted that the hemoglobin in the experimental groups decreased in group I by 7.9% in the II – 3.9% and III by 7.3% compared with the control group. Also, these groups distinguished downward trend eosinophils 1.8%, 1.5%, 2.05%. Then, as the number of segment-nuclear neutrophils contrast, in the first group increased by 18.2% in the second and third at 16.9% comparatively control. The number of monocytes in the second group doslfidniy the suspicion was higher by 66.3% and 0.3% higher than the physiological norm – it may indicate a high immune response.
The genesis of the ukrainian red dairy breed
Authors: Shevchuk N. P. 
Published: May 2017

For further improvement of livestock of the Ukrainian Red Dairy Breed it is important to study the genesis because this kind of cow is breed in many farms. Evaluation of the genesis of the breed was done step by step according to the results of crossing red steppe cattle with Angler’s bulls, Red Danish and Holstein breeds. For the research it has been used methods of retrospective analysis and variation statistics. It has been found that the breeding of the Ukrainian Red Diary Breed was done during five stages which have certain characteristics. During the first stage it was carried accumulation of breeding material using Angler breeders in herds of red steppe cattle. During the second phase of creating the breed it has been received a sufficient number of animals with different proportions of blood by Angler and Danish red breeds. According to the standard target average milk yield of cows of the Ukrainian Red dairy cattle is 4000 kg. The specialty of the third phase of breeding was the use of the gene pool of the Holstein breed as the most dairy productive caw. This helped to create intensive Holstein dairy type of the Ukrainian Red dairy breed. The average productivity of firstborn cows with different proportions of blood in Holstein red spoted breed is 5038–5638 kg of milk with fat in it up to 3.78–3.93%. The fourth step is completed with butterfat intrabreed and holshtynised types of Ukrainian dairy cattle and conducted their testing. At the fifth stage the work of heredity consolidation of newly formed breed is held and continues crossbreeding with Holstein breed bulls red spotted colour to increase milk production. Thus, animals of the Ukrainian Red Dairy Breed are characterized by a high level of productivity. At the farm «Colos 2011» in Mykolaiv region the milk yield of cows during 305 days and the third lactation is over 8565 kg of milk, the quantity of milk fat is 332 kg.
Functional outcomes of platelet rich plasma injections in plantar fascitis
Authors: Muralidharagopalan Niranjanan Raghavn , Loganathan Dinesh , Janakiram Iyer Krithika Meenakshi , Boopathikumar Kavin Kumar 
Published: May 2017

<p class="abstract"><strong>Background:</strong> The study was conducted to analyse the clinical outcomes following a single injection of platelet rich plasma, produced using autologous blood by standard double centrifugation technique in patients with plantar fasciitis who have not had relief using conservative methods.</p><p class="abstract"><strong>Methods:</strong> Between June 2013 and June 2015, 26 consecutive patients (Mean age 45.92) (11 males/15 females) who had symptoms for a minimum of 3 months and had failed conservative management options such as rest, anti-inflammatories and physiotherapy were given a single injection of PRP in the medial calcaneal tubercle.<strong></strong></p><p class="abstract"><strong>Results:</strong> The outcomes were measured using Visual analogue scale and Foot function index at pre injection, and at 1, 6 and 12 months post injection and results tabulated. All patients reported an improvement in VAS and FFI scores consistently. One superficial infection was encountered.</p><p><strong>Conclusions:</strong> Single injection of platelet rich plasma improves pain and function in the foot in patients suffering from plantar fasciitis when conservative management has failed. The synergism between various autologous growth factors may play a role in tendon healing. </p>