bluuebunny/crossref_metadata_2025_split huggingface dataset:
OPTIMISASI PRODUKSI ENZIM LAKASE PADA FERMENTASI KULTUR PADAT MENGGUNAKAN JAMUR PELAPUK PUTIH<i> Marasmius sp.</i> : PENGARUH UKURAN PARTIKEL, KELEMBAPAN, DAN KONSENTRASI Cu
Authors: Hanung Cornelius Damar , Osmond Ronald , Risdianto Hendro , Suhardi Sri Harjati , Setiadi Tjandra 
Published: August 2017

White rot fungi of Marasmius sp. is a fungus which produce laccase in high activity. Laccase is one of the ligninolityc enzymes that capable to degrade lignin. This ability can be used for the pretreatment of lignocellulosic materials in the bioethanol production. Laccase was produced in flask by batch process using Solid State Fermentation (SSF). The optimisation was conducted by statistically of full factorial design. The particle size, moisture content, and Cu concentration were investigated in this study. Rice straw was used as solid substrate and the glycerol was used as the carbon sources in modified Kirk medium. The results showed that particle size of rice straw did not affect significantly to the enzyme activity. The highest laccase activity of 4.45 IU/g dry weight was obtained at the moisture content of 61% and Cu concentration of 0.1 mM.Keywords: laccase, Marasmius sp., optimisation, rice straw, solid state fermentation ABSTRAKJamur pelapuk putih, Marasmius sp. merupakan jamur yang menghasilkan enzim lakase dengan aktivitas tinggi. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin. Kemampuan ini dapat digunakan untuk proses pengolahan awal bahan lignoselulosa pada pembuatan bioetanol. Produksi lakase dilakukan dalam labu dengan modus batch menggunakan fermentasi kultur padat. Optimisasi produksi enzim lakase dengan metode fermentasi padat dilakukan dengan rancangan percobaan faktorial penuh. Pengaruh ukuran partikel, kelembapan, dan konsentrasi Cu diuji dengan medium penyangga jerami dengan menambahkan gliserol dalam medium Kirk termodifikasi sebagai sumber karbon. Penelitian ini menunjukkan bahwa ukuran jerami tidak berpengaruh signifikan terhadap aktivitas enzim. Aktivitas enzim lakase maksimum terjadi pada saat kelembapan 61% dan konsentrasi Cu 0,1 mM dengan aktivitas enzim lakase/berat kering tertinggi mencapai 4,45 IU/g.Kata kunci: lakase, Marasmius sp., optimisasi, jerami, fermentasi kultur padat
Authors: Pacífico Juracy Machado , Müller Maria Cândida 
Published: August 2017

A Revista EDUCA - Revista Multidisciplinar em Educação inicia sua publicação em 2014, com duas edições e tem como missão promover a disseminação dos conhecimentos na área das Ciências da Educação. A EDUCA é um periódico científico vinculado ao Grupo de Pesquisa Multidisciplinar em Educação (Grupo EDUCA) e ao Programa de Pós-Graduação em Educação Escolar - Mestrado Profissional (PPGEE/MEPE), ambos vinculados ao Departamento de Ciências da Educação (DED) do Núcleo de Ciências Humanas (NCH) da Universidade Federal de Rondônia (UNIR).
Authors: Prates Claudia Aparecida , Müller Maria Cândida 
Published: August 2017

Este artigo é parte do estudo realizado durante o desenvolvimento da pesquisa de mestrado da primeira autora sobre formação continuada de docentes em grupos colaborativos de estudo e aprendizagem do professor. Apresenta os resultados preliminares da pesquisa exploratória sobre a formação acadêmica e complementar dos professores do Instituto Federal de Rondônia -IFRO - Câmpus Vilhena, participantes do grupo colaborativo de estudo sobre a temática avaliação da aprendizagem e que foram colaboradores da pesquisa. Distribui-se em três seções: a primeira traz algumas reflexões sobre formação continuada após a promulgação da Lei 9394/96. A segunda seção aborda a constituição de grupos colaborativos como possibilidade de formação continuada no contexto do IFRO - Câmpus Vilhena e a terceira apresenta o perfil dos participantes do grupo colaborativo sobre avaliação da aprendizagem.
Authors: Melo Márcia Maria Dantas Cabral de , Souza Fábio Barbosa de , Pires Iris Bruneia Fernandes , Cardoso Luis Henrique Gonçalves 
Published: August 2017

Objetivou-se analisar a visão dos formandos sobre o Projeto Pedagógico do curso de Odontologia da Universidade Federal de Pernambuco baseado nas Diretrizes Curriculares Nacionais e iniciado em 2010. Optou-se pelo estudo descritivo de delineamento transversal e abordagem quantitativa. A população estudada foi o universo dos concluintes (96) nos semestres letivos 2014.1 e 2014.2. A coleta de dados utilizou questionário estruturado, submetido a validação de face por especialistas. Dois pesquisadores treinados aplicaram o instrumento de coleta de dados junto aos alunos. Participaram 76 alunos, 72,4% eram mulheres e 59,2% tinham de 24 a 35 anos de idade. Houve aprovação da maioria das disciplinas dos três eixos formativos com valores de importância acima dos 50%. Obtiveram menores aprovações algumas disciplinas do eixo humanístico/social (Psicologia, Saúde Educação e Sociedade) e uma do eixo biológico (biofísica). Prevaleceram estratégias de ensino/aprendizagem conservadoras, nos eixos 1 e 2: aulas expositivas/discursivas (96,1%); no eixo 3: atividades clínicas (98,7%). Estágios em clínica básica e de especialidades tiveram importância de 90,8%, e na atenção básica 81,6%. A maioria demonstrou sentir-se capacitada para o exercício profissional. O projeto pedagógico obteve avaliação discente positiva e as deficiências formativas e os problemas apontados em disciplinas específicas merecem revisão.
Remineralization Potential of Three Tooth Pastes on Enamel Caries
Authors: Singhal Rajnish K. , Rai Balwant 
Published: August 2017

BACKGROUND: Different formulations of dentifrices exist in the market. Usually, single toothpaste is used by all family members including children. There is a big concern of fluoride ingestion with the toothpaste containing high fluoride content in children. Recently, new toothpaste (including toothpaste) with remineralization potential without fluoride content has been formulated.AIM: There is an urgent need to compare remineralization potential of this new formulation with the exiting dentifrices. Therefore, the present study has been undertaken to assess and compare the remineralization potential of three dentifrices with different compositions on artificially induced carious lesions in vitro by using scanning electron microscopy and polarised light microscopy.MATERIALS AND METHODS: The present in vitro study was conducted on 21 healthy extracted primary central incisor teeth surfaces, which were divided into three groups and were treated by three different dentifrices. Artificial demineralization was followed by remineralization using dentifrice slurry as per the group distribution. All the samples were studied for remineralization by using scanning electron microscopy and polarised light microscopy. Data were analysed using SPSS version 11 software.RESULTS: A significant difference was found between the remineralization potential of incudent toothpaste and other toothpaste groups based on the analysis of polarised light microscopy and stereomicroscope. The remineralizing ability of incudent toothpaste for artificial enamel lesions was found to be significantly higher than that of Colgate® and Crest toothpaste.CONCLUSIONS: The limitations of this study include, being a short term study, low sample size and in vitro experiment. incudent toothpaste has exhibited a higher remineralizing potential as compared to fluoride based toothpaste in our study.
The Effect of the Crystalline Lens on Central Vault After Implantable Collamer Lens Implantation
Authors: Qi Meng-ying , Chen Qian , Zeng Qing-yan 
Published: August 2017

PURPOSE:
To identify associations between crystalline lens–related factors and central vault after Implantable Collamer Lens (ICL) (Staar Surgical, Monrovia, CA) implantation.

METHODS:
This retrospective clinical study included 320 eyes from 186 patients who underwent ICL implantation surgery. At 1 year after surgery, the central vault was measured using anterior segment optical coherence tomography. Preoperative anterior chamber depth, lens thickness, lens position (lens position = anterior chamber depth + 1/2 lens thickness), and vault were analyzed to investigate the effects of lens-related factors on postoperative vault.

RESULTS:

The mean vault was 513 ± 215
µ
m at 1 year after surgery. Vault was positively correlated with preoperative anterior chamber depth (
r
= 0.495,
P
< .001) and lens position (
r
= 0.371,
P
< .001), but negatively correlated with lens thickness (
r
= −0.262,
P
< .001). Eyes with vaults of less than 250
µ
m had shallower anterior chambers, thicker lenses, and smaller lens position than eyes in the other two vault groups (which had vaults ≥ 250
µ
m) (
P
< .001). Eyes with both anterior chamber depth less than 3.1 mm and lens position less than 5.1 mm had greatly reduced vaults (
P
< .001).

CONCLUSIONS:
The crystalline lens could have an important influence on postoperative vault. Eyes with a shallower anterior chamber and a forward lens position will have lower vaults.

[
J Refract Surg.
2017;33(8):519–523.]
Capsule-Fixated Intraocular Lens Implantation in Small Pupil Cases
Authors: Schojai Merita , Schultz Tim , Burkhard Dick H. 
Published: August 2017

PURPOSE:
To describe a new technique for implantation of capsule-fixated intraocular lenses (IOLs) (FEMTIS; Oculentis, Berlin, Germany) in patients with small pupils.

METHODS:
In 4 eyes with small pupils, an anterior capsule–fixated IOL was implanted into the capsular bag after femtosecond laser treatment. The two large and two small flaps of the IOL were elevated to the front of the iris and the anterior capsule. Finally, the iris was flipped over the flaps to ensure a fixation of the capsule inside of the capsulotomy.

RESULTS:
In all cases, the implantation of anterior capsule–fixated IOLs was possible. No complications occurred during surgery or within the first months after surgery.

CONCLUSIONS:
With the described technique, capsulefixated IOLs can be implanted in eyes with small pupil easily and safely. This type of IOL has great potential to improve the refractive outcome by better prediction of the postoperative IOL position and eliminating IOL rotation after cataract surgery.

[
J Refract Surg.
2017;33(8):568–570.]
IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection
Authors: Garth Jaleesa M. , Reeder Kristen M. , Godwin Matthew S. , Mackel Joseph J. , Dunaway Chad W. , Blackburn Jonathan P. , Steele Chad 
Published: August 2017

Abstract
Members of the IL-1 family play protective and regulatory roles in immune defense against the opportunistic mold Aspergillus fumigatus. In this study, we investigated the IL-1 family member IL-33 in lung defense against A. fumigatus. IL-33 was detected in the naive lung, which further increased after exposure to A. fumigatus in a dectin-1–independent manner. Mice deficient in the receptor for IL-33 (Il1rl1−/−) unexpectedly demonstrated enhanced lung clearance of A. fumigatus. IL-33 functioned as a negative regulator of multiple inflammatory cytokines, as IL-1α, IL-1β, IL-6, IL-17A, and IL-22 were significantly elevated in fungal-exposed Il1rl1−/− mice. Subsequently, IL-33 administration to normal mice attenuated fungal-induced IL-17A and IL-22, but not IL-1α, IL-1β, or IL-6, production. IL-33–mediated regulation of IL-17A and IL-22 did not involve the modulation of IL-23 but rather PGE2; PGE2 was significantly increased in fungal-exposed Il1rl1−/− mice, and normal mice produced less PGE2 after fungal exposure when administered IL-33, suggesting that IL-33–mediated regulation of IL-17A and IL-22 occurred at the level of PGE2. This was confirmed by in vivo cyclooxygenase 2 inhibition, which attenuated fungal-induced IL-17A and IL-22, as well as IL-1α, IL-1β, and IL-6, production in Il1rl1−/− mice, resulting in impaired fungal clearance. We also show that a PGE2 receptor agonist increased, whereas a PGE2 synthase inhibitor decreased, the levels of IL-17A and IL-22 but not IL-1α, IL-1β, or IL-6. This study establishes novel mechanisms of innate IL-17A/IL-22 production via PGE2 and regulation of the PGE2/IL-17A/IL-22 axis via IL-33 signaling during lung fungal exposure.
Integrin-Linked Kinase Expression in Myeloid Cells Promotes Inflammatory Signaling during Experimental Colitis
Authors: Ahmed Afsar U. , Yim Howard C. H. , Alorro Mariah , Ernst Matthias , Williams Bryan R. G. 
Published: August 2017

Abstract
The pathology of inflammatory bowel diseases is driven by the inflammatory signaling pathways associated with mucosal epithelial damage. Myeloid cells are known to play an essential role in mediating epithelial inflammatory responses during injury. However, the precise role of these cells in stimulating intestinal inflammation and the subsequent tissue damage is unclear. In this article, we show that expression of integrin-linked kinase (ILK) in myeloid cells is critical for the epithelial inflammatory signaling during colitis induced by dextran sodium sulfate. Myeloid ILK (M-ILK) deficiency significantly ameliorates the pathology of experimental colitis. In response to dextran sodium sulfate, colonic infiltration of neutrophils and inflammatory cytokine production are impaired in M-ILK–deficient mice, and activation of epithelial NF-κB and PI3K signaling pathways are restricted by the M-ILK deficiency. In contrast, reduced epithelial damage in M-ILK–deficient mice is correlated with elevated levels of epithelial Stat3 activation and proliferation. Moreover, M-ILK–dependent inflammatory signaling in the mucosal epithelium can be therapeutically targeted by the pharmacological inhibition of ILK during experimental colitis. Collectively, these findings identify M-ILK as a critical regulator of epithelial inflammatory signaling pathways during colitis and, as a consequence, targeting M-ILK could provide therapeutic benefit.
Cutting Edge: IL-2–Induced Expression of the Amino Acid Transporters SLC1A5 and CD98 Is a Prerequisite for NKG2D-Mediated Activation of Human NK Cells
Authors: Jensen Helle , Potempa Marc , Gotthardt Dagmar , Lanier Lewis L. 
Published: August 2017

Abstract
Priming of human NK cells with IL-2 is necessary to render them functionally competent upon NKG2D engagement. We examined the underlying mechanisms that control NKG2D responsiveness in NK cells and found that IL-2 upregulates expression of the amino acid transporters SLC1A5 and CD98. Using specific inhibitors to block SLC1A5 and CD98 function, we found that production of IFN-γ and degranulation by CD56bright and CD56dim NK cells following NKG2D stimulation were dependent on both transporters. IL-2 priming increased the activity of mTORC1, and inhibition of mTORC1 abrogated the ability of the IL-2–primed NK cells to produce IFN-γ in response to NKG2D-mediated stimulation. This study identifies a series of IL-2–induced cellular changes that regulates the NKG2D responsiveness in human NK cells.
Enumerating Unlabelled Embeddings of Digraphs
Authors: Chen Yichao , Gao Xiaojian , Huang Yuanqiu 
Published: August 2017

AbstractA 2-cell embedding of an Eulerian digraph D into a closed surface is said to be directed if the boundary of each face is a directed closed walk in D. In this paper, a method is developed with the purpose of enumerating unlabelled embeddings for an Eulerian digraph. As an application, we obtain explicit formulas for the number of unlabelled embeddings of directed bouquets of cycles Bn, directed dipoles OD2n and for a class of regular tournaments T2n+1.
Possible climatic implications of high-altitude black carbon emissions
Authors: Govardhan Gaurav , Satheesh Sreedharan Krishnakumari , Nanjundiah Ravi , Moorthy Krishnaswamy Krishna , Babu Surendran Suresh 
Published: August 2017

Abstract. On account of its strong absorption of solar and terrestrial radiation, black carbon (BC) aerosol is known to impact large-scale systems, such as the Asian monsoon and the Himalayan glaciers, in addition to affecting the thermal structure of the lower atmosphere. While most studies focus on the near-surface abundance and impacts of BC, our study examines the implications of sharp and confined layers of high BC concentration (called elevated BC layers) at altitudes more than 4 km over the Indian region using the online regional chemistry transport model (WRF-Chem) simulations. These elevated BC layers were revealed in the recent in situ measurements using high-altitude balloons carried out on 17 March 2010, 8 January 2011 and 25 April 2011. Our study demonstrates that high-flying aircraft (with emissions from the regionally fine-tuned MACCity inventory) are the most likely cause of these elevated BC layers. Furthermore, we show that such aircraft-emitted BC can be transported to upper tropospheric or lower stratospheric heights ( ∼  17 km) aided by the strong monsoonal convection occurring over the region, which is known to overshoot the tropical tropopause, leading to the injection of tropospheric air mass (along with its constituent aerosols) into the stratosphere. We show observational evidence for such an intrusion of tropospheric BC into the stratosphere over the Indian region using extinction coefficient and particle depolarisation ratio data from CALIOP Lidar on-board the CALIPSO satellite. We hypothesise that such intrusions of BC into the lower stratosphere and its consequent longer residence time in the stratosphere have significant implications for stratospheric ozone, especially considering the already reported ozone-depleting potential of BC.
10Be systematics in the Tsangpo-Brahmaputra catchment: the cosmogenic nuclide legacy of the eastern Himalayan syntaxis
Authors: Lupker Maarten , Lavé Jérôme , France-Lanord Christian , Christl Marcus , Bourlès Didier , Carcaillet Julien , Maden Colin , Wieler Rainer , Rahman Mustafizur , Bezbaruah Devojit , Xiaohan Liu 
Published: August 2017

Abstract. The Tsangpo-Brahmaputra River drains the eastern part of the Himalayan range and flows from the Tibetan Plateau through the eastern Himalayan syntaxis downstream to the Indo-Gangetic floodplain and the Bay of Bengal. As such, it is a unique natural laboratory to study how denudation and sediment production processes are transferred to river detrital signals. In this study, we present a new 10Be data set to constrain denudation rates across the catchment and to quantify the impact of rapid erosion within the syntaxis region on cosmogenic nuclide budgets and signals. The measured 10Be denudation rates span around 2 orders of magnitude across individual catchments (ranging from 0.03 to > 4 mm yr−1) and sharply increase as the Tsangpo-Brahmaputra flows across the eastern Himalaya. The increase in denudation rates, however, occurs  ∼  150 km downstream of the Namche Barwa–Gyala Peri massif (NBGPm), an area which has been previously characterized by extremely high erosion and exhumation rates. We suggest that this downstream lag is mainly due to the physical abrasion of coarse-grained, low 10Be concentration, landslide material produced within the syntaxis that dilutes the upstream high-concentration 10Be flux from the Tibetan Plateau only after abrasion has transferred sediment to the studied sand fraction. A simple abrasion model produces typical lag distances of 50 to 150 km compatible with our observations. Abrasion effects reduce the spatial resolution over which denudation can be constrained in the eastern Himalayan syntaxis. In addition, we also highlight that denudation rate estimates are dependent on the sediment connectivity, storage, and quartz content of the upstream Tibetan Plateau part of the catchment, which tends to lead to an overestimation of downstream denudation rates. While no direct 10Be denudation measurements were made in the syntaxis, the dilution of the upstream 10Be signal, measured in Tsangpo-Brahmaputra sediments, provides constraints on the denudation rates in that region. These denudation estimates range from ca. 2 to 5 mm yr−1 for the entire syntaxis and ca. 4 to 28 mm yr−1 for the NBGPm, which is significantly higher than other large catchments. Overall, 10Be concentrations measured at the outlet of the Tsangpo-Brahmaputra in Bangladesh suggest a sediment flux between 780 and 1430 Mt yr−1 equivalent to a denudation rate between 0.7 and 1.2 mm yr−1 for the entire catchment.
Enviro-HIRLAM online integrated meteorology–chemistry modelling system: strategy, methodology, developments and applications (v7.2)
Authors: Baklanov Alexander , Smith Korsholm Ulrik , Nuterman Roman , Mahura Alexander , Nielsen Kristian Pagh , Sass Bent Hansen , Rasmussen Alix , Zakey Ashraf , Kaas Eigil , Kurganskiy Alexander , Sørensen Brian , González-Aparicio Iratxe 
Published: August 2017

Abstract. The Environment – High Resolution Limited Area Model (Enviro-HIRLAM) is developed as a fully online integrated numerical weather prediction (NWP) and atmospheric chemical transport (ACT) model for research and forecasting of joint meteorological, chemical and biological weather. The integrated modelling system is developed by the Danish Meteorological Institute (DMI) in collaboration with several European universities. It is the baseline system in the HIRLAM Chemical Branch and used in several countries and different applications. The development was initiated at DMI more than 15 years ago. The model is based on the HIRLAM NWP model with online integrated pollutant transport and dispersion, chemistry, aerosol dynamics, deposition and atmospheric composition feedbacks. To make the model suitable for chemical weather forecasting in urban areas, the meteorological part was improved by implementation of urban parameterisations. The dynamical core was improved by implementing a locally mass-conserving semi-Lagrangian numerical advection scheme, which improves forecast accuracy and model performance. The current version (7.2), in comparison with previous versions, has a more advanced and cost-efficient chemistry, aerosol multi-compound approach, aerosol feedbacks (direct and semi-direct) on radiation and (first and second indirect effects) on cloud microphysics. Since 2004, the Enviro-HIRLAM has been used for different studies, including operational pollen forecasting for Denmark since 2009 and operational forecasting atmospheric composition with downscaling for China since 2017. Following the main research and development strategy, further model developments will be extended towards the new NWP platform – HARMONIE. Different aspects of online coupling methodology, research strategy and possible applications of the modelling system, and fit-for-purpose model configurations for the meteorological and air quality communities are discussed.
Encantando com a poesia: uma experiência criativa na Escola Daniel Batista
Authors: De Queiroz Souza Kênia Paulino , Pinho Maria José De 
Published: August 2017

As práticas pedagógicas contemporâneas no cenário educativo com uma visão ampliada e transformadora possibilitam ressignificações em suas vivências educacionais. Com esse olhar global, o presente relato de experiências objetiva polinizar ações que caracterizem práticas pedagógicas
criativas, a partir dos projetos que norteiam o funcionamento dos anos iniciais do Ensino Fundamental. Este artigo é um recorte da pesquisa de mestrado em educação ‘Práticas pedagógicas criativas: uma perspectiva transdisciplinar na escola do século XXI’ desenvolvida na Escola Municipal de Tempo Integral Daniel Batista. A qual apresenta metodologicamente uma abordagem qualitativa exploratória, direcionada pelo estudo de caso. Na coleta de dados usou o questionário VADECRIE (Instrumento de valoração das práticas criativas da Rede Internacional de Escolas Criativas - RIEC) e as técnicas de entrevistas semiestruturadas, observação direta e análise de documentos (Projeto Político Pedagógico, Plano de Ação e projetos pedagógicos). De acordo com a análise parcial dos dados, a Escola Daniel Batista desenvolve uma variedade de ações diferenciadas e integradoras. A sua prática pedagógica a partir dos projetos e, principalmente, do ‘projeto Declamadores de poesia’ é realizada em equipe com espontaneidade, determinação,
criatividade e com o reconhecimento da importância de seus objetivos educacionais estarem conectados com a vida.