bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphometric study of adipocytes on breast cancer by means of photonic microscopy and image analysis
Authors: Suárez–Nájera Luis Eduardo , Chanona–Pérez José Jorge , Valdivia–Flores Alejandra , Marrero–Rodríguez Daniel , Salcedo–Vargas Mauricio , García–Ruiz David Israel , Castro– Reyes Marco Antonio 
Published: November 2017

AbstractWorldwide, breast cancer (BrCa) is currently the leading cause of deaths associated to malignant lesions in adult women. Given that some studies have mentioned that peritumoral adipocytes may contribute to breast carcinogenesis, present work sought to quantitative evaluate the morphometry of these cells in a group of adult women. Three thousand six hundred sixty four breast adipocytes, that came from biopsies of a group of adult females with different types of breast carcinomas (ductal, lobular, and mixed) and one with normal tissues, were evaluated through an image analysis (IA) process regarding six morphometric descriptors: area (A), perimeter (P), Feret diameter (FD), aspect ratio (AR), roundness factor (RF), and fractal dimension of cellular contour (FDC). Data showed that the adipocytes of the normal tissues group were bigger (A: 3398 ± 2331 µm2, P: 239 ± 83 µm, and FD: 79.9 ± 24.5 µm) than those from BrCa samples (A: 2860 ± 1933 µm2, P: 214 ± 66 µm, and FD: 73.2 ± 22.5 µm), and presented a more irregular contour (FDC of 1.370 ± 0.037 for normal group and of 1.335 ± 0.049 for the oncologic one). Moreover, it could be accounted that adipocytes of mixed carcinomas were largest (FD: 75.1 ± 22.4 µm) than those of lobular lesions (FD: 61.6 ± 22.6 µm), while the adipocytes of ductal carcinomas were the most oval (AR: 1.421 ± 0.524) and roughest (FDC: 1.324 ± 0.050) cells. IA results suggest that BrCa lesions can be categorized through a quantitative morphometric evaluation of peritumoral adipocytes. These findings could let the development of an analytical tool to help the Pathologist to enhance the accuracy of the oncologic diagnose.
Visualization of the native shape of bodipy‐labeled DNA inEscherichia coliby correlative microscopy
Authors: Loukanov Alexandre , Mladenova Polina , Toshev Svetlin , Udono Hibiki , Nakabayashi Seiichiro 
Published: November 2017

AbstractThe native shape and intracellular distribution of newly synthesized DNA was visualized by correlative (light and electron) microscopy in ice embedded whole cells ofEscherichia coli. For that purpose, the commercially available modified nucleoside triphosphate named BODIPY® FL‐14‐dUTP was enzymatically incorporatedin vivointo the genome ofE. colimutant K12 strain, which cannot synthesize thymine. The successful incorporation of this thymidine analogue was confirmed first by fluorescence microscope, where the cells were stained in the typical for bodipy green color. Later the preselected labeledE. coliwere observed by Hilbert Differential Transmission Electron Microscope (HDC TEM) and the distribution of elemental boron (contained in bodipy) was visualized at high‐resolution by an electron spectroscopic imaging (ESI) technique. The practical detection limit of boron was found to be around 5 ∼ 10 mmol/kg in area of 0.1 μm2, which demonstrated that ESI is a suitable approach to study the cytochemistry and location of labeled nucleic fragments within the cytoplasmic chromosomal area. In addition, the fine cellular fibrous and chromosomal ultrastructures were revealedin situby combing of phase‐plate HDC TEM and ESI. The obtained results conclude that the correlation between fluorescent microscopy with phase‐plate HDC TEM and ESI is a powerful approach to explore the structural and conformation dynamics of DNA replication machinery in frozen cells close to the living state.
Perfusion measures for symptom severity and differential outcome of revascularization in limb ischemia: Preliminary results with arterial spin labeling reactive hyperemia
Authors: Chen Hou‐Jen , Roy Trisha L. , Wright Graham A. 
Published: November 2017

BackgroundPreviously, a theoretical model based on microvascular physiology was established to facilitate the interpretation of calf perfusion dynamics recorded by arterial spin labeling (ASL).PurposeTo investigate the clinical relevance of novel perfusion indices by comparing them to the symptoms, response to revascularization, and short‐term functional outcome in patients with peripheral arterial disease (PAD).Study TypeProspective cohort study.PopulationNineteen patients with PAD.Field Strength/SequencePulsed ASL at 3T.AssessmentThe mid‐calf reactive hyperemia induced by 2 minutes of arterial occlusion was recorded in PAD patients. The perfusion responses were characterized by the peak, time‐to‐peak, and physiological model‐derived indices including the baseline perfusion fr, arterial resistance Ra, and compliance Ca, and sensitivity gATP and response time τATP of downstream microvasculature to metabolic stress. These indices were compared to the disease severity and outcome within 6 months after revascularization assessed by self‐reported symptoms and the ankle‐brachial index. Disease severity was categorized as asymptomatic, claudication, or critical limb ischemia. The outcome was categorized as symptom resolved or limited improvement.Statistical TestsSeverity and outcome groups were compared using Mann–Whitney and Kruskal–Wallis tests with Holm–Sidak adjustments.ResultsThe peak perfusion decreased and model arterial resistance increased progressively with increasing severity of limb ischemia (P = 0.0402 and 0.0413, respectively). Eleven patients had a successful endovascular procedure, including six patients who had symptoms resolved, four patients who had remaining leg pain, and one patient lost to follow‐up. The subjects with limited improvement had significantly lower preintervention microvascular sensitivity gATP than those with symptoms resolved (8.72 ± 1.46 vs. 4.93 ± 0.91, P = 0.0466).Data ConclusionASL reactive hyperemia reflects multiple aspects of the pathophysiology. Measures of macrovascular arterial disease are related to the manifested symptom severity, whereas preintervention gATP associated with microvascular dysfunction is related to prognosis following revascularization.Level of Evidence: 1Technical Efficacy: Stage 4J. Magn. Reson. Imaging 2018;47:1578–1588.
Detection of liver fibrosis using qualitative and quantitative MR elastography compared to liver surface nodularity measurement, gadoxetic acid uptake, and serum markers
Authors: Besa Cecilia , Wagner Mathilde , Lo Grace , Gordic Sonja , Chatterji Manjil , Kennedy Paul , Stueck Ashley , Thung Swan , Babb James , Smith Andrew , Taouli Bachir 
Published: November 2017

BackgroundMultiparametric magnetic resonance imaging (mpMRI) combining different techniques such as MR elastography (MRE) has emerged as a noninvasive approach to diagnose and stage liver fibrosis with high accuracy allowing for anatomical and functional information.PurposeTo assess the diagnostic performance of mpMRI including qualitative and quantitative assessment of MRE, liver surface nodularity (LSN) measurement, hepatic enhancement ratios postgadoxetic acid, and serum markers (APRI, FIB‐4) for the detection of liver fibrosis.Study TypeIRB‐approved retrospective.SubjectsEighty‐three adult patients.Field Strength/Sequence1.5T and 3.0T MR systems. MRE and T1‐weighted postgadoxetic acid sequences.AssessmentTwo independent observers analyzed qualitative color‐coded MRE maps on a scale of 0–3. Regions of interest were drawn to measure liver stiffness on MRE stiffness maps and on pre‐ and postcontrast T1‐weighted images to measure hepatic enhancement ratios. Software was used to generate LSN measurements. Histopathology was used as the reference standard for diagnosis of liver fibrosis in all patients.Statistical TestsA multivariable logistic analysis was performed to identify independent predictors of liver fibrosis. Receiver operating characteristic (ROC) analysis evaluated the performance of each imaging technique for detection of fibrosis, in comparison with serum markers.ResultsLiver stiffness measured with MRE provided the strongest correlation with histopathologic fibrosis stage (r = 0.74, P < 0.001), and the highest diagnostic performance for detection of stages F2‐F4, F3‐F4, and F4 (areas under the curve [AUCs] of 0.87, 0.91, and 0.89, respectively, P < 0.001) compared to other methods. Qualitative assessment of MRE maps showed fair to good accuracy for detection of fibrosis (AUC range 0.76–0.84). Multivariable logistic analysis identified liver stiffness and FIB‐4 as independent predictors of fibrosis with AUCs of 0.90 (F2‐F4), 0.93 (F3‐F4) and 0.92 (F4) when combined.Data ConclusionLiver stiffness measured with MRE showed the best performance for detection of liver fibrosis compared to LSN and gadoxetic acid uptake, with slight improvement when combined with FIB‐4.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2018;47:1552–1561.
DMSO as a mobile phase additive enhances detection of ubiquitination sites by nano‐LC‐ESI‐MS/MS
Authors: Doellinger Joerg , Grossegesse Marica , Nitsche Andreas , Lasch Peter 
Published: November 2017

AbstractLarge‐scale detection of ubiquitination sites in whole cell proteomes using nano–liquid chromatography coupled to tandem mass spectrometry is a well‐established technique that has deepened the understanding of protein degradation processes in eukaryotic cells. Ubiquitination sites are usually identified by detection of Lys‐ɛ‐Gly‐Gly (K‐ɛ‐GG)‐remnant peptides, which are generated by tryptic digestion of proteomes. We show in this application note that dimethyl sulfoxide addition to the liquid chromatography mobile phase enhances identification rates of K‐ɛ‐GG peptides by more than 100% due to an increase of peptide signal intensities. The gain in the number of ubiquitination site identifications exceeds by far the gain that has been published for other posttranslational modifications, namely, phosphorylation and acetylation.
Converging early responses to brain injury pave the road to epileptogenesis
Authors: Neuberger Eric J. , Gupta Akshay , Subramanian Deepak , Korgaonkar Akshata A. , Santhakumar Vijayalakshmi 
Published: November 2017

AbstractEpilepsy, characterized by recurrent seizures and abnormal electrical activity in the brain, is one of the most prevalent brain disorders. Over two million people in the United States have been diagnosed with epilepsy and 3% of the general population will be diagnosed with it at some point in their lives. While most developmental epilepsies occur due to genetic predisposition, a class of “acquired” epilepsies results from a variety of brain insults. A leading etiological factor for epilepsy that is currently on the rise is traumatic brain injury (TBI), which accounts for up to 20% of all symptomatic epilepsies. Remarkably, the presence of an identified early insult that constitutes a risk for development of epilepsy provides a therapeutic window in which the pathological processes associated with brain injury can be manipulated to limit the subsequent development of recurrent seizure activity and epilepsy. Recent studies have revealed diverse pathologies, including enhanced excitability, activated immune signaling, cell death, and enhanced neurogenesis within a week after injury, suggesting a period of heightened adaptive and maladaptive plasticity. An integrated understanding of these processes and their cellular and molecular underpinnings could lead to novel targets to arrest epileptogenesis after trauma. This review attempts to highlight and relate the diverse early changes after trauma and their role in development of epilepsy and suggests potential strategies to limit neurological complications in the injured brain.
The reciprocal relationship between job insecurity and depressive symptoms: A latent transition analysis
Authors: Vander Elst Tinne , Notelaers Guy , Skogstad Anders 
Published: November 2017

SummaryPrevious studies on the relationship between job insecurity and depressive symptoms have mainly focused on the stressor‐to‐strain effect from job insecurity to depressive symptoms, on rather secure and healthy employees, and on rank‐order relationships. This is not entirely in line with stress theories suggesting intraindividual and reciprocal relationships between high levels of stressors and strain. In reply, this study investigated whether high levels of job insecurity were related to subsequent high levels of depressive symptoms, and vice versa. Cross‐lagged dual process latent Markov model analysis with 3‐wave data (time lags of 2 and 3 years) from a representative sample of the Norwegian working force (N = 2,539) revealed 5 latent states of job insecurity and 6 latent states of depressive symptoms. As hypothesized, a reciprocal relationship between the “high job insecurity” state and the “depressed” state was found: Previously highly job‐insecure employees were more likely to be depressed at the next measurement point (OR = 42.54), and employees labeled as depressed were more likely to experience high job insecurity later on (OR = 69.92). This study contributes to stress theory by demonstrating that stressors and strain may relate differently depending on the level of stressor and strain experienced.
Chemical characterization of 21 species of marine macroalgae common in Norwegian waters: benefits of and limitations to their potential use in food and feed
Authors: Biancarosa Irene , Belghit Ikram , Bruckner Christian G , Liland Nina S , Waagbø Rune , Amlund Heidi , Heesch Svenja , Lock Erik‐Jan 
Published: November 2017

AbstractBACKGROUNDIn the past few years, much effort has been invested into developing a new blue economy based on harvesting, cultivating and processing marine macroalgae in Norway. Macroalgae have high potential for a wide range of applications, e.g. as source of pharmaceuticals, production of biofuels or as food and feed. However, data on the chemical composition of macroalgae from Norwegian waters are scant. This study was designed to characterize the chemical composition of 21 algal species. Both macro‐ and micronutrients were analysed. Concentrations of heavy metals and the metalloid arsenic in the algae were also quantified.RESULTSThe results confirm that marine macroalgae contain nutrients which are relevant for both human and animal nutrition, the concentrations whereof are highly dependent on species. Although heavy metals and arsenic were detected in the algae studied, concentrations were mostly below maximum allowed levels set by food and feed legislation in the EU.CONCLUSIONThis study provides chemical data on a wide range of algal species covering the three taxonomic groups (brown, red and green algae) and discusses both benefits of and potential limitations to their use for food and feed purposes. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
In vitro digestibility, free and bound phenolic profiles and antioxidant activity of thermally treated Eragrostis tef L
Authors: Koubová Eva , Mrázková Martina , Sumczynski Daniela , Orsavová Jana 
Published: November 2017

AbstractBACKGROUNDTotal phenolic content, phenolic profile and antioxidant activity were determined in free and bound phenolic fractions of thermally treated brown and white teff grains.RESULTSPhenolic content in raw brown and white teff (1540 and 992 mg gallic acid equivalent kg−1) as well as antioxidant activity (6.3 and 5.5 mmol trolox equivalent kg−1) were higher in free phenolic fractions. The most significant decrease in total phenolics was observed after application of the sous‐vide method (35% for brown teff and 11% for white teff). Main free phenolics of heat‐treated teff were ferulic, protocatechuic, p‐coumaric and ellagic acids, rutin and epigallocatechin. Main bound phenolics were ferulic, gallic, sinapic and ellagic acids, catechin and epigallocatechin. The detrimental effect on free and bound quercetin and bound cinnamic acid concentrations was also examined during heat treatment. Thermally treated brown teff showed a high level of in vitro organic matter digestibility if water cooking and rice cooker (both 99.5%) and sous‐vide (96.5%) methods were applied.CONCLUSIONThe sous‐vide method may be recommended as the most suitable hydrothermal treatment for grains of teff when compared with water cooking and rice cooker methods. © 2017 Society of Chemical Industry
Prediction of benzo[a]pyrene content of smoked sausage using back‐propagation artificial neural network
Authors: Chen Yan , Cai Kezhou , Tu Zehui , Nie Wen , Ji Tuo , Hu Bing , Chen Conggui , Jiang Shaotong 
Published: November 2017

AbstractBACKGROUNDBenzo[a]pyrene (BaP), a potent mutagen and carcinogen, is reported to be present in processed meat products and, in particular, in smoked meat. However, few methods exist for predictive determination of the BaP content of smoked meats such as sausage. In this study, an artificial neural network (ANN) model based on the back‐propagation (BP) algorithm was used to predict the BaP content of smoked sausage.RESULTSThe results showed that the BP network based on the Levenberg–Marquardt algorithm was the best suited for creating a nonlinear map between the input and output parameters. The optimal network structure was 3‐7‐1 and the learning rate was 0.6. This BP‐ANN model allowed for accurate predictions, with the correlation coefficients (R) for the experimentally determined training, validation, test and global data sets being 0.94, 0.96, 0.95 and 0.95 respectively. The validation performance was 0.013, suggesting that the proposed BP‐ANN may be used to predictively detect the BaP content of smoked meat products.CONCLUSIONAn effective predictive model was constructed for estimation of the BaP content of smoked sausage using ANN modeling techniques, which shows potential to predict the BaP content in smoked sausage. © 2017 Society of Chemical Industry
Elevated brain natriuretic peptide (BNP) is an early marker for patients at risk for complications after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS + HIPEC)
Authors: Fisher Sarah B. , Rafeeq Safia , Hess Ken , Grotz Travis E. , Mansfield Paul , Royal Richard , Badgwell Brian , Fleming Jason , Fournier Keith , Mann Gary N. 
Published: November 2017

BackgroundElevated BNP is associated with adverse cardiac outcomes after noncardiac surgery. We assessed BNP values as markers of perioperative fluid status and their correlation with major/cardiopulmonary (CP) complications following CRS + HIPEC.MethodsFluid balance, BNP levels, and morbidity data were collected for all patients undergoing CRS + HIPEC between 6/2014 and 2/2016.ResultsOne hundred and twenty‐nine patients underwent CRS + HIPEC for appendiceal adenocarcinoma (n = 99), mesothelioma (n = 16), and colon cancer (n = 14). Less than 10% had CP comorbidities. The median PCI was 14 (range 4‐39); 89% underwent CC0/1 resection (n = 115). Median blood loss (EBL) was 497 mL (50‐2700). Major complications (Clavien III‐V) occurred in 16 (12%), CP in 17 (13%), and major/CP in 24 (18%). Thirty‐day mortality occurred in 2 (1.5%). Elevated BNP on POD1 correlated with increased risk of major/CP complications (OR 2.2, P = 0.052). This was most pronounced in the 25 patients receiving cisplatin: for each 100 unit increase in POD1 BNP the OR for major/CP complication was 7.4 versus 1.2 for the remaining patients, P = 0.083. Multivariate analysis identified increased EBL (OR 4.1 P = 0.011) and a trend toward increased BNP on POD1 (OR for each 100 unit increase 2.0, P = 0.10) as risk factors for major/CP complications.ConclusionsPostoperative BNP measurement after CRS + HIPEC may guide postoperative fluid resuscitation and facilitate identification of patients at risk for major and/or cardiopulmonary complications.
Real‐time detection system for tumor localization during minimally invasive surgery for gastric and colon cancer removal: In vivo feasibility study in a swine model
Authors: Choi Won Jung , Moon Jin‐Hee , Min Jae Seok , Song Yong Keun , Lee Seung A , Ahn Jin Woo , Lee Sang Hun , Jung Ha Chul 
Published: November 2017

Background and ObjectivesDuring minimally invasive surgery (MIS), it is impossible to directly detect marked clips around tumors via palpation. Therefore, we developed a novel method and device using Radio Frequency IDentification (RFID) technology to detect the position of clips during minimally invasive gastrectomy or colectomy.MethodsThe feasibility of the RFID‐based detection system was evaluated in an animal experiment consisting of seven swine. The primary outcome was to successfully detect the location of RFID clips in the stomach and colon. The secondary outcome measures were to detect time (time during the intracorporeal detection of the RFID clip), and accuracy (distance between the RFID clip and the detected site).ResultsA total of 25 detection attempts (14 in the stomach and 11 in the colon) using the RFID antenna had a 100% success rate. The median detection time was 32.5 s (range, 15‐119 s) for the stomach and 28.0 s (range, 8‐87 s) for the colon. The median detection distance was 6.5 mm (range, 4‐18 mm) for the stomach and 6.0 mm (range, 3‐13 mm) for the colon.ConclusionsWe demonstrated favorable results for a RFID system that detects the position of gastric and colon tumors in real‐time during MIS.
Expression of programmed death‐1 in sentinel lymph nodes of breast cancer
Authors: Tatara Takashi , Mukohara Toru , Shimono Yohei , Yamasaki Takashi , Imamura Yoshinori , Funakoshi Yohei , Toyoda Masanori , Kiyota Naomi , Takao Shintaro , Kono Seishi , Kakeji Yoshihiro , Minami Hironobu 
Published: November 2017

Background and ObjectivesTo explore whether lymphocytes in sentinel lymph nodes (SLNs) are highly exposed to tumor neoantigens and thus express high level of programmed death 1 (PD‐1), we examined PD‐1 expression in SLNs and non‐sentinel regional lymph nodes (non‐SLNs) in breast cancer.MethodsWe performed PD‐1 immunohistochemistry in two cohorts: 40 metastasis‐negative SLNs including 10 patients for each subtype (luminal A‐like, luminal B‐like, HER2, and triple negative breast cancer [TNBC]); and 25 pairs of metastasis‐positive SLNs and non‐SLNs (10 luminal A‐like, 10 luminal B‐like, and 5 TNBC).ResultsAmong 40 metastasis‐negative SLNs, 34 and 6 samples were PD‐1 intensity grade 1 (low) and 2 (high), respectively. PD‐1 intensity correlated with PD‐1‐positive lymphocyte numbers (P = 0.005); TNBC had the highest PD‐1 lymphocyte numbers among all subtypes. The median PD‐1‐positive lymphocyte number was higher in SLNs than non‐SLNs. In most cases, more lymphocytes in SLNs expressed PD‐1 than those in non‐SLNs (P < 0.0001).ConclusionsTNBC had the greatest PD‐1 expression among all subtypes, and metastasis‐positive SLNs had more PD‐1‐positive lymphocytes than downstream non‐SLNs. These data suggested that lymphocytes in SLNs are activated following exposure to tumor neoantigens and thus tumor specific, and could be utilized as a biomarker platform.