bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Incretin-Secreting Microbe Improves Metabolic Dysfunction in High-Fat Diet Fed Rodents
Authors: Ryan Paul M. , Patterson Elaine , Kent Robert M. , Stack Helena , O’Connor Paula M. , Murphy Kiera , Peterson Veronica L. , Mandal Rupasri , Wishart David S. , Dinan Timothy G. , Cryan John F. , Seeley Randy J. , Stanton Catherine , Ross R. Paul 
Published: October 2017

AbstactThe gut hormone glucagon-like peptide (GLP)-1 and its analogues represent a new generation of anti-diabetic drugs, which have also demonstrated propensity to modulate host lipid metabolism. Despite this, drugs of this nature are currently limited to intramuscular administration routes due to intestinal degradation. The aim of this study was to design a recombinant microbial delivery vector for a GLP-1 analogue and assess the efficacy of the therapeutic in improving host glucose, lipid and cholesterol metabolism in diet induced obese rodents. Diet-induced obese animals received either Lactobacillus paracasei NFBC 338 transformed to express a long-acting analogue of GLP-1 or the isogenic control microbe which solely harbored the pNZ44 plasmid. Short-term GLP-1 microbe intervention in rats reduced serum low-density lipoprotein cholesterol, triglycerides and triglyceride-rich lipoprotein cholesterol substantially. Conversely, extended GLP-1 microbe intervention improved glucose-dependent insulin secretion, glucose metabolism and cholesterol metabolism, compared to the high-fat control group. Interestingly, the microbe significantly attenuated the adiposity associated with the model and altered the serum lipidome, independently of GLP-1 secretion. These data indicate that recombinant incretin-secreting microbes may offer a novel and safe means of managing cholesterol metabolism and diet induced dyslipidaemia, as well as insulin sensitivity in metabolic dysfunction.
MiR-200c regulates tumor growth and chemosensitivity to cisplatin in osteosarcoma by targeting AKT2
Authors: Liu Yang , Zhu Shu-Tao , Wang Xiao , Deng Jun , Li Wei-Hua , Zhang Peng , Liu Bing-Shan 
Published: October 2017

AbstractMicroRNAs (miRNAs) expression aberration has been discovered in almost all human cancers, thus offering a group of potential diagnostic markers, prognostic factors and therapeutic targets in tumorigenesis. Now our data showed that miR-200c, which is downregulated in osteosarcoma tissues, drives chemosensitivity to cisplatin in osteosarcoma. We demonstrated that AKT2 is a direct target of miR-200c, Spearman’s rank correlation analysis showed that the expression levels of AKT2 and miR-200c in 35 pairs of osteosarcoma specimens were inversely correlated. Moreover, miR-200c inhibited cell proliferation and cell migration. Taken together, for the first time, our results demonstrate that miR-200c plays a significant role in osteosarcoma tumor growth and chemosensitivity by regulating AKT2, which may provide a novel therapeutic strategy for treatment of osteosarcoma.
Vector-independent transmembrane transport of oligodeoxyribonucleotides involves p38 mitogen activated protein kinase phosphorylation
Authors: Peng Minyuan , Li Yanming , Zhang Jian , Wu Yong , Yang Xiaoyang , Lei Ye , Ye Mao , Liu Jing , Han Xu , Kuang Yijin , Zhao Xielan , Chen Fangping 
Published: October 2017

AbstractThe main roles of equilibrative nucleoside transporters (ENTs) and concentrative nucleoside transporters (CNTs) are to transfer single nucleosides and analogues for the nucleic acid salvage pathway. Oligodeoxyribonucleotides (ODNs) can be transported into the cytoplasm or nucleus of cells under certain conditions. Among ODNs composed of a single type of nucleotide, the transport efficiency differs with the length and nucleotide composition of the ODNs and varies in different types of leukaemia cells; among the 5 tested random sequence ODNs and 3 aptamers with varying sequences, the data showed that some sequences were associated with significantly higher transport efficiency than others. The transport of ODNs was sodium, energy, and pH-independent, membrane protein-dependent, substrate nonspecific for ODNs and 4-nitrobenzylthioinosine (NBMPR)-insensitive, but it showed a low sensitivity to dipyridamole (IC50 = 35.44 µmol/L), distinguishing it from ENT1-4 and CNTs. The delivery efficiency of ODNs was superior to that of Lipofection and Nucleofection, demonstrating its potential applications in research or therapeutics. Moreover, this process was associated with p38 mitogen activated protein kinase (p38MAPK) instead of c-Jun N-terminal kinase (JNK) signalling pathways. We have denoted ODN transmembrane transport as equilibrative nucleic acid transport (ENAT). Overall, these findings indicate a new approach and mechanism for transmembrane transport of ODNs.
Preconditioning with far-infrared irradiation enhances proliferation, cell survival, and migration of rat bone marrow-derived stem cells via CXCR4-ERK pathways
Authors: Jeong Yun-Mi , Cheng Xian Wu , Lee Sora , Lee Kyung Hye , Cho Haneul , Kang Jung Hee , Kim Weon 
Published: October 2017

AbstractFar-infrared radiation (FIR) has been shown to exert positive effects on the cardiovascular system. However, the biological effects of FIR on bone marrow-derived stem cells (BMSCs) are not understood. In the present study, BMSCs were isolated from rat femur bone marrow and cultured in vitro. To investigate the effects of an FIR generator with an energy flux of 0.13 mW/cm2 on rat BMSCs, survival of BMSCs was measured by crystal violet staining, and cell proliferation was additionally measured using Ez-Cytox cell viability, EdU, and Brd U assays. FIR preconditioning was found to significantly increase BMSC proliferation and survival against H2O2. The scratch and transwell migration assays showed that FIR preconditioning resulted in an increase in BMSC migration. qRT-PCR and Western blot analyses demonstrated that FIR upregulated Nanog, Sox2, c-Kit, Nkx2.5, and CXCR4 at both the mRNA and protein levels. Consistent with these observations, PD98059 (an ERK inhibitor) and AMD3100 (a CXCR4 inhibitor) prevented the activation of CXCR4/ERK and blocked the cell proliferation and migration induced by FIR. Overall, these findings provide the first evidence that FIR confers a real and significant benefit on the preconditioning of BMSCs, and might lead to novel strategies for improving BMSC therapy for cardiac ischemia.
Graphene enhanced silicon/carbon composite as anode for high performance lithium-ion batteries
Authors: Li Xiaohui , Wu Mengqiang , Feng Tingting , Xu Ziqiang , Qin Jingang , Chen Cheng , Tu Chengyang , Wang Dongxia 
Published: October 2017

Silicon-based anode materials for lithium ion batteries (LIBs) have become a hot research topic due to their remarkably high theoretical capacity (4200 mA h g−1).
Extension of the Modified Julia Olefination on Carboxylic Acid Derivatives: Scope and Applications
Authors: Gueyrard David 
Published: October 2017

This account relates our work in the field of modified Julia olefination to extend this very useful olefination method to carboxylic acid derivatives. Since our preliminary results on lactones in 2005, the reaction has been extended to a large range of derivatives (lactams, imides and anhydrides) through an intra- or intermolecular process leading to a great variety of structures (enol ethers, enamides and exo enol esters). This article will also focus on the application of this methodology for the preparation of biologically interesting compounds and/or total syntheses of natural products such as C-disaccharide, bistramide A, jaspine B and maculalactone B.1	Introduction2	Modified Julia Olefination on Lactones2.1	Methylene Enol Ether Synthesis2.2	Substituted Enol Ether Synthesis2.3	Monofluorinated Enol Ether Synthesis2.4	Difluorinated Enol Ether Synthesis3	Applications3.1	Spiroketal Synthesis3.2	Spirocompound Synthesis3.3	Pseudodisaccharide Synthesis3.4	Total Synthesis of Jaspine B4	Modified Julia Olefination on Other Carboxylic Acid Derivatives4.1	Lactam Olefination and Spiroaminal Synthesis4.2	Bicyclic Enamide Synthesis by Intramolecular Modified Julia Olefination on Imides4.3	Modified Julia Olefination on Anhydrides5	Conclusion
Investigational Medical Therapies for Endometriosis: Current Data and Future Trends
Authors: Zhang Ran , Lebovic Dan , Kavoussi Shahryar 
Published: October 2017

AbstractCurrent mainstays of medical therapy for endometriosis preclude fertility or may have unwanted side effects. Therefore, research has been underway to find alternatives to current options which may be applied to future medical treatment. The focus of this review is that of investigational hormonal and nonhormonal medical therapies based on in vitro and in vivo animal models of endometriosis as well as in human endometrial and endometriotic cells in vitro. Assessment of the safety and efficacy of such novel medical treatments have been, and continue to be, necessary prior to clinical trials.
Choosing wisely? Multiple Sclerosis and Laboratory Screening for Autoimmune Differential Diagnoses
Authors: Becker Jana , Geffken Mareike , Diehl Rolf , Berlit Peter , Krämer Markus 
Published: October 2017

Abstract
Background A variety of diseases can mimic the presentation of multiple sclerosis (MS). Diagnosing MS requires the exclusion of conditions considered in the differential diagnosis of MS. The guidelines of the German Society of Neurology (DGN) recommend analyzing 13 mandatory and 9 optional laboratory parameters. The mandatory recommendations include antinuclear antibodies (ANA) as well as antibodies against double-stranded DNA (anti-dsDNA Abs)).
Methods In a cohort of MS outpatients, those patient records were analyzed in which test results for all or at least some of the recommended laboratory parameters were available. In addition to these laboratory parameters, MRI data, family history, clinical presentation, evoked potentials, and CSF findings were analyzed. Furthermore, a questionnaire was used to prospectively collect information about rheumatological symptoms relevant to the differential diagnosis of MS.
Results In 197 of the included 554 patients, at least some of the recommended differential diagnostic laboratory parameters were available. In 59.4% of these patients, ANA titers ≥ 1:80 were detected. Neither ANA nor ANCA nor anti-dsDNA abs were able to distinguish between MS and non-MS. In 124 MS patients, 54.8% had positive ANA titers ≥1:80. MS patients with these titers suffered more frequently from autoimmune diseases and were more likely to have a family history of MS. Of the remaining 73 patients without MS, 67.1% were ANA positive. In this patient population, the ANA-positive patients were more frequently diagnosed with psychiatric diseases. In the prospectively surveyed MS patients with an ANA titer >1:160, no rheumatologic disease was diagnosed during the average follow-up period of 1.9 years.
Conclusion In line with the "Choosing Wisely" recommendations of the American College of Rheumatology, mandatory laboratory screening appears to offer no benefit to the initial diagnosis of multiple sclerosis, unless a specific differential diagnosis is to be excluded.
Facile Synthesis and Characterization of L-Aspartic Acid Coated Iron Oxide Magnetic Nanoparticles (IONPs) For Biomedical Applications
Authors: Salehiabar Marziyeh , Nosrati Hamed , Davaran Soodabeh , Danafar Hossein , Manjili Hamidreza 
Published: October 2017

AbstractNatural L-aspartic acid coated iron oxide magnetic nanoparticles (Asp@IONPs) were prepared by a one pot, in-situ and green co–precipitation method in an aqueous medium. Functionalized iron oxide magnetic nanoparticles (IONPs) were characterized by Vibrating Sample Magnetometer (VSM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of IONPs was also investigated on HEK-293 cell lines. The results showed that the zeta potential of Asp@IONPs was about −21.1 mV and the average size was 17.80±3.09 nm. Cell toxicity results show that as prepared IONPs are biocompatible. Asp@IONPs show the possibility of using these nanoparticles in the development of in vitro and in vivo biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.
Targeting autophagy inhibits melanoma growth by enhancing NK cells infiltration in a CCL5-dependent manner
Authors: Mgrditchian Takouhie , Arakelian Tsolere , Paggetti Jérôme , Noman Muhammad Zaeem , Viry Elodie , Moussay Etienne , Van Moer Kris , Kreis Stephanie , Guerin Coralie , Buart Stephanie , Robert Caroline , Borg Christophe , Vielh Philippe , Chouaib Salem , Berchem Guy , Janji Bassam 
Published: October 2017

SignificanceThe failure in achieving a durable clinical immune response against cancer cells depends on the ability of cancer cells to establish a microenvironment that prevent cytotoxic immune cells to infiltrate tumors and kill cancer cells. Therefore, the key approach to achieving successful antitumor immune response is to harness strategies allowing the reorientation of immune cells to the tumor. Herein we reveal that inhibiting autophagy induces a massive infiltration of natural killer immune cells into the tumor bed, and a subsequent dramatic decrease in the tumor volume of melanomas. These results highlight the role of targeting autophagy in breaking the immunosuppressive tumor microenvironment barrier, thus allowing the infiltration of natural killer cells into the tumor to kill cancer cells.
Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact
Authors: Ray-Soni Ananya , Mooney Rachel A. , Landick Robert 
Published: October 2017

SignificanceRNA polymerase (RNAP), like many cellular processors of information in DNA and RNA, is a complex macromolecular machine whose multiple structural modules and domains undergo poorly understood conformational changes that mediate information processing. We investigated the role of one such mobile module, the polymorphous trigger loop (TL) of RNAP, in intrinsic transcription termination by bacterial RNAP. The TL folds into a helical hairpin to promote RNA synthesis, but also is proposed to aid termination. By separating effects of the TL and of TL variants on termination from effects on RNA synthesis, we established that TL flexibility, not the helical hairpin conformation, facilitates rearrangements of RNAP leading to termination. Our results illustrate how kinetic assays can help dissect complex macromolecular machines.
PTIP chromatin regulator controls development and activation of B cell subsets to license humoral immunity in mice
Authors: Su Dan , Vanhee Stijn , Soria Rebeca , Gyllenbäck Elin Jaensson , Starnes Linda M. , Højfeldt Martina Kubec , Pedersen Gabriel K. , Yuan Joan , Daniel Jeremy A. 
Published: October 2017

Significance
To provide optimal host defense, the full spectrum of antibody-based immunity requires natural antibodies and immunization-induced antigen-specific antibodies. Here we show that the PTIP (Pax transactivation domain-interacting protein) chromatin regulator is induced by B cell activation to potentiate the establishment of steady-state and postimmune serum antibody levels. It does so by promoting activation-associated proliferation and differentiation of all the major B cell subsets, at least in part, through regulating the NF-κB pathway. With the genetic basis still unknown for a majority of patients with common variable immunodeficiency, further work investigating how PTIP controls cell signaling may generate valuable new insight for human health and disease.
Hedgehog signaling regulates ciliary localization of mouse odorant receptors
Authors: Maurya Devendra Kumar , Bohm Staffan , Alenius Mattias 
Published: October 2017

Significance
Cells communicate with each other via signaling molecules that bind to surface receptors of responding cells. One example is Hedgehog (Hh) signaling, which is known for its regulatory role of tissue development and maintenance. Here we identify a Hh function, which is to regulate the sense of smell. Hh signaling modulates odorant responsiveness by regulating the accumulation of odorant receptors (ORs) in the olfactory ciliary compartment, which is where odorant detection is initiated. Since the mechanism appears evolutionarily conserved, even though the structures of ORs are not, the results open for the interesting possibility that Hh signaling may regulate protein transport and stimuli responsiveness also in other types of neurons.
Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes
Authors: Ludwig Barbara , Ludwig Stefan , Steffen Anja , Knauf Yvonne , Zimerman Baruch , Heinke Sophie , Lehmann Susann , Schubert Undine , Schmid Janine , Bleyer Martina , Schönmann Uwe , Colton Clark K. , Bonifacio Ezio , Solimena Michele , Reichel Andreas , Schally Andrew V. , Rotem Avi , Barkai Uriel , Grinberg-Rashi Helena , Kaup Franz-Josef , Avni Yuval , Jones Peter , Bornstein Stefan R. 
Published: October 2017

Significance
Diabetes mellitus type 1 is an autoimmune disease that results in irreversible destruction of insulin-producing beta cells. Substantial advances have been made in beta cell replacement therapies during the last decades. However, lack of eligible donor organs and the need for chronic immunosuppression to prevent rejection critically limit widespread application of these strategies. In this manuscript, we present an experimental study using a bioartificial pancreas device for the transplantation of xenogeneic islet without affecting the immune system in nonhuman primates. We could demonstrate stable graft function and adequate glucose-regulated insulin secretion without the need for immunosuppressive medication. This strategy opens up new avenues for more widespread and safe application of various cell-based therapies.
Growth patterns for shape-shifting elastic bilayers
Authors: van Rees Wim M. , Vouga Etienne , Mahadevan L. 
Published: October 2017

Significance
Many biological forms, such as leaves, flowers, and faces, are shaped by complex growth patterns. How can we prescribe the rules of growth on a simple surface so that it will morph into a flower or a face? Here, we solve this inverse problem of designing the growth patterns for an anisotropically growing elastic bilayer structure and prove that it can be used to achieve any target surface shape from any reference shape. We demonstrate the applicability of this result via the computational design of growth patterns for animal, vegetable, and mineral surfaces—a face, a flower, and a canyon. Our solution provides algorithms for engineering complex functional shapes in tissues, and actuation systems in soft robotics, and elsewhere.
Descriptive and diachronic aspects of the Old Irish quotative marker ol
Authors: García Castillero Carlos 
Published: October 2017

Abstract
The most frequent strategy of direct reported speech in Old Irish (OIr) is characterized by the use of a constituent which is known in this paper as Reported Speaker, which consists of the element ol introducing the noun or the tonic pronoun referring to the person whose words are quoted. This paper offers a formal and pragmatic description of the Old Irish Reported Speaker, paying special attention to its basically nominal character and to its frequent coreferential association with first-person referents in the quote. The OIr quotative marker ol is diachronically explained as a further use of the OIr multifunctional element ol, which is essentially a preposition (‘beyond’) secondarily used as the marker of the NP standard of comparison and as a clausal connective.
Managing transcreation projects
Authors: Pedersen Daniel 
Published: October 2017

Abstract
This paper investigates the translation spaces of a very specific translation practice, namely transcreation. In a marketing context, transcreation is usually concerned with the adaptation of advertising material into several different languages or for different markets. The paper is based on an ethnographic field study carried out at a marketing implementation agency in London, during which a group of transcreation managers was followed over a period of four weeks. The study relies mainly on observations of the interactions between the employees of the above-mentioned agency and their partners as well as on the researcher’s own participation in some of the agency’s work-related activities. As an activity, transcreation often involves two or more writers. These writers are most likely to be physically separated, but as the data from this study show, a transcreation agency can serve as a case for joint, situated efforts.
Novel steroidal saponins from Dioscorea esculenta (Togedokoro)
Authors: Lee Hyoung Jae , Watanabe Bunta , Nakayasu Masaru , Onjo Michio , Sugimoto Yukihiro , Mizutani Masaharu 
Published: October 2017

Abstract
Fifteen steroidal saponins 1–15, which include 4 furostanol glycosides 1–3 and 15, and 11 spirostanol glycosides 4–14, were isolated from the tubers and leaves of lesser yam (Dioscorea esculenta, Togedokoro). Their structures were identified by nuclear magnetic resonance and liquid chromatography mass spectroscopy. Four steroidal saponins 9, 11, 14, and 15 were found to be novel compounds.
‘Being green is worthless if others are not’: the effect of descriptive norms on pro-environmental behaviour is mediated by outcome expectancy / ‘Ser verde no vale de nada si los demás no lo son’: el efecto de las normas descriptivas sobre el comportamiento proambiental está mediado por la expectativa de resultado
Authors: Moussaoui Lisa S. , Desrichard Olivier 
Published: October 2017

Descriptive norm effect has been explained in terms of either normative or informative social influence. This research investigates another possible mechanism in the case of collective goals. Because changing one’s behaviour — e.g., to protect the environment — will be effective only if other people also change their behaviour, their number will influence the outcome expectancy for that behaviour. Hence, we postulated that the effect of descriptive norm on adoption of a pro-environmental behaviour is mediated by outcome expectancy. Two studies showed that outcome expectancy mediated the effect of descriptive norm on intention to buy an energy-efficient refrigerator only in the case of a collective goal (vs. an individual goal) (Study 1, N = 787), and that the effect of descriptive norm on intention (with respect to a collective goal) can be reversed if an optimal uptake rate is mentioned, this interaction being mediated by outcome expectancy (Study 2, N = 400). These results suggest that the effect of descriptive norm, classically found in studies of pro-environmental behaviours, occurs because it gives people a sense that their action will have an impact.