bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-terminal sumoylation of centromeric histone H3 variant Cse4 regulates its proteolysis to prevent mislocalization to non-centromeric chromatin
Authors: Ohkuni Kentaro , Levy-Myers Reuben , Warren Jack , Au Wei-Chun , Takahashi Yoshimitsu , Baker Richard E. , Basrai Munira A. 
Published: August 2017

AbstractStringent regulation of cellular levels of evolutionarily conserved centromeric histone H3 variant (CENP-A in humans, CID in flies, Cse4 in yeast) prevents its mislocalization to non-centromeric chromatin. Overexpression and mislocalization of CENP-A has been observed in cancers and leads to aneuploidy in yeast, flies, and human cells. Ubiquitin-mediated proteolysis of Cse4 by E3 ligases such as Psh1 and Sumo-Targeted Ubiquitin Ligase (STUbL) Slx5 prevent mislocalization of Cse4. Previously, we identified Siz1 and Siz2 as the major E3 ligases for sumoylation of Cse4. In this study, we identify lysine 65 (K65) in Cse4 as a SUMO site and show that sumoylation of Cse4 K65 regulates its ubiquitin-mediated proteolysis by Slx5. Strains expressingcse4 K65Rexhibit reduced levels of sumoylated and ubiquitinated Cse4in vivo. Furthermore, co-immunoprecipitation experiments reveal reduced interaction of cse4 K65R with Slx5. Defects in sumoylation of cse4 K65R contribute to increased stability and mislocalization of cse4 K65R under normal physiological conditions. Based on the increased stability of cse4 K65R inpsh1∆strains but not inslx5∆strains, we conclude that Slx5 targets sumoylated Cse4 K65 for ubiquitination-mediated proteolysis independent of Psh1. In summary, we have identified and characterized the physiological role of Cse4 sumoylation and determined that sumoylation of Cse4 K65 regulates ubiquitin-mediated proteolysis and prevents mislocalization of Cse4 which is required for genome stability.
Network inference reveals novel connections in pathways regulating growth and defense in the yeast salt response
Authors: MacGilvray Matthew E. , Shishkova Evgenia , Chasman Deborah , Place Michael , Gitter Anthony , Coon Joshua J. , Gasch Audrey P. 
Published: August 2017

ABSTRACTCells respond to stressful conditions by coordinating a complex, multi-faceted response that spans many levels of physiology. Much of the response is coordinated by changes in protein phosphorylation. Although the regulators of transcriptome changes during stress are well characterized inSaccharomyces cerevisiae, the upstream regulatory network controlling protein phosphorylation is less well dissected. Here, we developed a computational approach to infer the signaling network that regulates phosphorylation changes in response to salt stress. The method uses integer linear programming (ILP) to integrate stress-responsive phospho-proteome responses in wild-type and mutant strains, predicted phosphorylation motifs on groups of coregulated peptides, and published protein interaction data. A key advance is that by grouping peptides into submodules before inference, the method can overcome missing protein interactions in published datasets to predict novel, stress-dependent protein interactions and phosphorylation events. The network we inferred predicted new regulatory connections between stress-activated and growth-regulating pathways and suggested mechanisms coordinating metabolism, cell-cycle progression, and growth during stress. We confirmed several network predictions with co-immunoprecipitations coupled with mass-spectrometry protein identification and mutant phospho-proteomic analysis. Results show that the cAMP-phosphodiesterase Pde2 physically interacts with many stress-regulated transcription factors targeted by PKA, and that reduced phosphorylation of those factors during stress requires the Rck2 kinase that we show physically interacts with Pde2. Together, our work shows how a high-quality computational network model can facilitate discovery of new pathway interactions during osmotic stress.
Population-based mechanistic modeling allows for quantitative predictions of drug responses across cell types
Authors: Gong Jingqi QX , Sobie Eric A 
Published: August 2017

ABSTRACTQuantitative mismatches between human physiology and experimental models can present serious limitations for the development of effective therapeutics. We addressed this issue, in the context of cardiac electrophysiology, through mechanistic mathematical modeling combined with statistical analyses. Physiological metrics were simulated in heterogeneous populations describing cardiac myocytes from adult ventricles and those derived from induced pluripotent stem cells (iPSC-CMs). These simulated measures were used to construct a cross-cell type regression model that predicts adult myocyte drug responses from iPSC-CM behaviors. We found that quantitatively accurate predictions of responses to selective or non-selective drugs could be generated based on iPSC-CM responses and that the method can be extended to predict drug responses in diseased as well as healthy cells. This cross-cell type model can be of great value in drug development, and the approach, which can be applied to other fields, represents an important strategy for overcoming experimental model limitations.
Dlic1deletion impaired cerebellar development in mouse
Authors: Kong Shanshan , Tao Wufan 
Published: August 2017

AbstractThe cerebellum is an important model system to study Central Nervous System, it has a striking morphology consisting of folia separated by fissures of different lengths, and the molecules controlling the process of cerebellar development have been unclear. In this study, we report an unrecognized contribution ofDlic1to the postnatal development of murine cerebellum.Dlic1encodes a light intermediate chain of cytoplasmic dynein 1, it is a subunit unique to the cytoplasmic form of dynein, and how it contributes to dynein function is not fully understood. Our data show thatDlic1deletion leads to impaired cerebellar development, foliation and fissuration, the volume of the cerebellum was evidently reduced. Consistent with the histological defects,Dlic1knockout mice show defective motor coordination, this strongly indicates the fundamental role ofDlic1in cerebellar development and function. Purkinje cells are located within the cerebellum and form synapses with granule cells. InDlic1-/-mice, Purkinje cells display reduced dendritic complexity and the distribution of dynein in Purkinje cell layer is changed. We also found thatDlic1deletion reduced the protein levels of dynein subunits and leads to the defective assembling of dynein subunits. These data indicate thatDlic1may affect cerebellar development through affecting the normal function of dynein.AbbreviationsDlic1cytoplasmic dynein 1 light intermediate chainCNScentral nervous systemMEFmouse embryo fibroblast
Rebel with a cause
Authors: Wong Wilson , Chu May 
Published: August 2017

Purpose
The purpose of this paper is to examine the cause and nature of the 2014 Umbrella Movement in Hong Kong and the role of the youth in the movement.

Design/methodology/approach
This paper analyzes the relationship between the serious social and economic problems in Hong Kong, of which the youth often feel the greatest impact, and the demands for a real democratic system made by the Umbrella Movement. It examines the structural roots of the Movement and the role of youths in it.

Findings
The Umbrella Movement is not simply a movement of anger and frustration, but also a movement reflecting some of the serious and legitimate concerns of the people of Hong Kong, especially the youth, who have a high and growing sense of local identity and citizenship. The movement links the major policy and social problems of the post-Handover era to its root cause, which is an undemocratic political system combined with crony capitalism. Unfortunately, the approach taken by the government toward the Movement has been to emphasize its illegal nature and to attempt to divide the opposition by adopting “united front” strategies that pay no serious attention to the problems the Movement has raised. Eventually, this approach will only lead to an outcome of “strong government, weak society” where strong government action in opposition to reformers will weaken the cohesion of society and expose the actual weakness of the state that has no popular legitimacy, going on to create more chaos as a result of its weak governance.

Originality/value
This paper will help both scholars and policymakers to understand the structural and root causes of the Umbrella Movement in Hong Kong and explain why Hong Kong is suffering from a serious problem of weak governance.
Dynamic interactions between sustainability and competitiveness in construction firms
Authors: Chang Rui-Dong , Zuo Jian , Soebarto Veronica , Zhao Zhen-Yu , Zillante George 
Published: August 2017

Purpose
Sustainability and competitiveness have received extensive attentions. Despite a large number of studies on sustainability and competitiveness in the construction industry, little research has been conducted to holistically explore the interactions between these two concepts. From a dynamic transition perspective, the purpose of this paper is to link sustainability and competitiveness of construction firms by developing a Sustainability-Competitiveness Dynamic Interaction Framework (SCDIF).

Design/methodology/approach
Conceptual theory-building approach was adopted to develop the conceptual framework. It is an iterative analysis and synthesis process, which involves reading literature, identifying commonalities and differences, synthesizing, proposing an initial framework, collecting additional literature, and revisiting and revising the framework.

Findings
There are complex interactions between sustainability and competitiveness of construction firms. This leads to uncertain relationships between sustainability and competitiveness, which is context dependent. Under evolving economic and socio-political environments, sustainability and competitiveness of construction firms could transition from mutually exclusive to mutually supportive, and finally merge into “sustainable competitiveness.”

Research limitations/implications
A SCDIF proposed in this study demonstrates that the interactions between sustainability and competitiveness evolves according to the evolving economic and socio-political environments and firms’ strategies, and thus the relationships and interactions between sustainability and competitiveness are context dependent. This framework helps corporate managers to understand how corporate sustainability and competitiveness interact with each other, thereby informing their decision-making of sustainability strategy. Similarly, the framework provides useful references for policymakers to understand the mechanisms of transitioning industries toward sustainable competitiveness.

Originality/value
The proposed framework offers a new perspective for understanding sustainability and competitiveness. From the dynamic transition perspective, this study effectively illustrates that the interactions between sustainability and competitiveness evolves according to the evolving economic and socio-political environments and firms’ strategies. Compared to existing approaches, the dynamic and holistic approach proposed in this paper provides the capacity to capture the complexity of sustainability and competitiveness.
Stakeholder competency in evaluating the environmental impacts of infrastructure projects using BIM
Authors: Murphy Martina Elizabeth , Nahod Maja-Marija 
Published: August 2017

Purpose
Building information modelling (BIM) literature reveals a growing interest in the development of a competency-based approach to manage the long-term goals of BIM implementation in infrastructure projects. One long-term goal is mitigation of environmental impacts (EIs). It is proposed that by integrating environmental systems within the BIM model, the technology can act as an early warning indicator to assist stakeholders identify and evaluate EIs before they become critical to delivery. The purpose of this paper is to assess the effectiveness of BIM in identifying EIs on infrastructure projects and investigate the correlation between stakeholder competency and evaluation of EIs.

Design/methodology/approach
In all, 71 informants that have relevant experience in infrastructure projects were investigated using a two-stage methodology comprising a questionnaire to determine the BIM indicators used to identify EIs and the stakeholder competencies required to assess and evaluate EIs and Behavioural Event Interviews (BEIs) to validate the competencies identified.

Findings
The findings showed that risk assessments are the most critical early warning indicator in identifying EIs specifically when implemented within the cost management process. The key stakeholder competencies required to successfully evaluate EIs were identified as project organisation and building equitable relationships. BEIs showed these stakeholders to also have high levels of behavioural and contextual awareness. This suggests that, contrary to perceived perception, successful management of EIs is more dependent on collaborative working than the acquisition of technical skills. Findings also indicated that Croatian BIM stakeholders are less experienced than UK BIM stakeholders in project implementation and delivery and that less experienced BIM stakeholders require more emphasis on technical knowledge whilst the importance of “soft skills” is more apparent in experienced stakeholders, notably amongst the UK participants.

Originality/value
The implications for infrastructure projects show that effective management of EIs can be achieved through alignment of the BIM model with the cost management plan implemented by stakeholders working collaboratively. Hence, the strategic focus for AEC companies working on infrastructure projects should be the development of staff interpersonal competencies rather than solely on project goals and/or an over-emphasis on technical skills.
Managing the expectations of external stakeholders in construction projects
Authors: Chan Albert P.C. , Oppong Goodenough D. 
Published: August 2017

Purpose
The consideration of external stakeholders has proven to be more critical than internal stakeholders in construction projects. The purpose of this paper is to present the diverse expectations of external stakeholder groups, i.e. governmental authorities, general public, and affected local communities, in construction projects. The practical steps to manage the expectations are also outlined.

Design/methodology/approach
A three-stage methodology was adopted for the review. The primary terms “stakeholder,” “project participants,” or “project environment” were first searched in four popularly search engines and eight top journals that publish construction research to retrieve publications. After a second-stage filtering process, the selected data were then analyzed and reviewed in line with the objectives.

Findings
In total, 49 common expectations were identified and classified. The results indicate that each stakeholder group pursues expectations in line with the social, environmental, and economic sustainability objectives. For effective management, project managers (PMs) must know stakeholder opportunities and threats, fulfill social responsibilities, establish common goals, apply appropriate strategies, and enhance stakeholder satisfaction.

Research limitations/implications
The identified expectations are only based on the selected publications. Even though the expectations have been categorized in line with the triple bottom line model, the relative importance of the expectations cannot be ascertained since there is no empirical support.

Practical implications
PMs can play safe by acknowledging the stakeholder expectations and employ such strategies to curtail resulting impacts and maximize mutual benefits. The list of expectations could also be used to promote equitable value optimization in projects, enhance needs fulfillment, and facilitate the evaluation of external stakeholder satisfaction.

Originality/value
This study provides a comprehensive checklist of construction stakeholder expectations which hitherto, is lacked in the literature. Moreover, practical steps to manage the expectations of external stakeholders have been discussed.
Disruption causes and disruption management in supply chains with Chinese suppliers
Authors: Durach Christian F. , Glasen Patrick C. , Straube Frank 
Published: August 2017

PurposeThe purpose of this paper is to identify and rank supply chain disruption causes for Western buying firms in the Chinese market; to identify supplier-relationship-specific mitigation strategies to avoid and resist such disruptions; and to develop and propose a framework of relational supply chain disruption management with Chinese suppliers.Design/methodology/approachTwo group exercises with 42 representatives from Western manufacturing buying firms and nine in-depth interviews were conducted. The group exercises applied the nominal group technique.FindingsThe authors identified and ranked 22 disruption causes in China for Western buying firms. Evaluating the five most urgent causes, 43 mitigation strategies could be identified that build on implementing strategic relationships with Chinese suppliers. A framework of relational supply chain disruption management for Western buying firms was developed with six propositions on primary constructs, mediators, and moderators, highlighting guanxi as a fundamental construct of relations within the Chinese culture.Research limitations/implicationsThe findings contribute to theory development at the intersection of risk management and culture. Quantitative testing of the proposed relationships in the framework is needed to derive more reliable conclusions.Practical implicationsThe study depicts how cultural differences between Chinese suppliers and Western buyers influence relational supply chain disruption management strategies. Using the study findings, managers of Western buying firms are informed regarding the most pressing disruption causes in the Chinese market and the value and strategic use of Chinese-supplier relationships.Originality/valueThe study provides a valuable contribution to the scant body of literature on disruption management in supply chains with Chinese suppliers. It contributes to our understanding of a successful risk management in the presence of cultural differences.
Developing SCM framework associated with IT-enabled SC network capabilities
Authors: Chen Shyh-Shiuh , Ou-Yang Chao , Chou Tzu-Chuan 
Published: August 2017

PurposeThe purpose of this paper is to conceptualize how information technology (IT) enables supply chain (SC) network capabilities, which is to capitalize on SC’s existing set of resources and, at the same time, manage new combinations of SC resources to meet future market needs. The paper also develops SCM framework associated with IT-enabled SC network capabilities.Design/methodology/approachThrough a case study of a leading Taiwanese petrochemical corporation, qualitative data were gathered on the IT-related SC management practices, in terms of network resource mobilizing and adaptive co-management arrangements to enable SC network capability. This research is based primarily on the interviews of the case company, supplemented by archived documents, published books, and in-depth observations.FindingsBased on the evidence from the case, this study inductively develops a model that includes the operating processes with IT-enabled activities to achieve ambidextrous SC network capability, and the relevant framework functions in network resources and co-management activities include information co-governance, information interoperability, community engagement strategy, cyber-physical dexterity, and control enactment, which lead the SC alliances improvements for dynamic environmental changes.Practical implicationsPractitioners may derive strategies and tactics from the current findings to help them implement innovative information technologies and setup SC framework, during SC network capability development, to achieve SC’s sustainable competence in a dynamic market.Originality/valueResearchers and practitioners may obtain a more complete view of IT-enabled SC network capability development. The proposed model reveals that developing IT-enabled SC network capabilities is a dynamic process whereby an organization’s major SC managerial activities are divided into specific network resource mobilizing and adaptive co-management arrangements.
Dependence, trust, and 3PL integration: an empirical study in China
Authors: Huo Baofeng , Liu Chen , Chen Haozhe , Zhao Xiande 
Published: August 2017

PurposeThe purpose of this paper is to investigate relationships among dependence, trust, and integration in the Chinese 3PL context. 3PL integration is manifested in two key dimensions: information sharing and process coordination.Design/methodology/approachThis study develops a dependence-trust-3PL integration-performance model and tests it using structural equation modeling with survey data collected from 361 companies in the Greater China area (i.e. mainland China, Hong Kong, and Taiwan).FindingsThe results show that switch dependence is indirectly related to information sharing and process coordination through goodwill trust, while goal dependence has direct links with both integrative behaviors. The authors also found that only goodwill trust mediates the relationship between dependence and integrative behaviors, while ability trust does not mediate any relationships. Finally, the analysis validated the direct link between process coordination and financial performance, but did not find a significant link between information sharing and financial performance.Originality/valueDifferent from most previous studies on similar topics, this study examines the impacts of different types of dependence and trust on different 3PL integration dimensions. As a result, the findings are more specific and have direct relevance to effective 3PL relationship management in China.
Public health expenditure, GDP and the elderly population: a comparative study
Authors: Pascual-Saez Marta , Cantarero-Prieto David , Castañeda Daniela 
Published: August 2017

Purpose
The correlation between health care expenditure, gross domestic product (GDP) and population over 65 years (understood as share of the elderly) is a key question for health economics and demographic impact. The purpose of this paper is to study the role of ageing society to curb rising health care expenditures along the Spanish regions over the period 2002-2013, identifying their geographic differences and explain them based on GDP differences.

Design/methodology/approach
Cointegration technique is used in order to test if there is a statistically significant connection between variables.

Findings
They are similar to some obtained when using unit root test. In particular, the authors find how the elderly positively affects health care expenditure per capita.

Practical implications
The findings suggest that any cooperation policies should aim at improving the access of people to health care services based on public health care expenditures.

Originality/value
To the best of the knowledge this is one of the first studies which suggest different results by Spanish regions due to mature decentralized system in recent years.