bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics analysis of human tears from aqueous-deficient and evaporative dry eye patients
Authors: Perumal Natarajan , Funke Sebastian , Pfeiffer Norbert , Grus Franz H. 
Published: July 2016

AbstractDespite the high global prevalence of dry eye syndrome (DES), the fundamental processes underlying this pathology remain largely unexplored. Therefore, this study endeavoured to investigate in-depth the tear proteome of DES patients employing the mass spectrometry (MS)-based proteomic strategies. Eighty patients were recruited and subdivided into three major DES subgroups, which are the aqueous-deficient (DRYaq), evaporative (DRYlip) and a combination of the two (DRYaqlip), as well as healthy subjects (CTRL). Discovery proteomics strategy was employed to identify large number of significantly differentially expressed tear proteins in DRYlipvs. CTRL, DRYaqvs. CTRL and DRYaqlipvs. CTRL with 22, 58 and 67 proteins, respectively. Biological functional analysis demonstrated for the first time that various metabolic processes were highly expressed in DRYaq and DRYaqlip, which might modulate various other known processes, especially the inflammatory and immune processes. Targeted proteomics strategy verified that 13 major proteins were differentially expressed in specific DES subgroups, comprising of PRR4, ZG16B, SCGB2A1, DMBT1, PROL1, LACRT, ALDH3A1, ENO1, TF, S100A8, S100A9, PEBP1 and ORM1. In conclusion, this study had explored in-depth the pathology of DES by unravelling various new fundamental processes and the major proteins responsible for the maintenance of tear film stability.
Hypoxia-mediated impaired erythrocyte Lands’ Cycle is pathogenic for sickle cell disease
Authors: Wu Hongyu , Bogdanov Mikhail , Zhang Yujin , Sun Kaiqi , Zhao Shushan , Song Anren , Luo Renna , Parchim Nicholas F. , Liu Hong , Huang Aji , Adebiyi Morayo G. , Jin Jianping , Alexander Danny C. , Milburn Michael V. , Idowu Modupe , Juneja Harinder S. , Kellems Rodney E. , Dowhan William , Xia Yang 
Published: July 2016

AbstractAlthough Lands’ cycle was discovered in 1958, its function and cellular regulation in membrane homeostasis under physiological and pathological conditions remain largely unknown. Nonbiased high throughput metabolomic profiling revealed that Lands’ cycle was impaired leading to significantly elevated erythrocyte membrane lysophosphatidylcholine (LysoPC) content and circulating and erythrocyte arachidonic acid (AA) in mice with sickle cell disease (SCD), a prevalent hemolytic genetic disorder. Correcting imbalanced Lands’ cycle by knockdown of phospholipase 2 (cPLA2) or overexpression of lysophosphatidycholine acyltransferase 1 (LPCAT1), two key enzymes of Lands’ cycle in hematopoietic stem cells, reduced elevated erythrocyte membrane LysoPC content and circulating AA levels and attenuated sickling, inflammation and tissue damage in SCD chimeras. Human translational studies validated SCD mouse findings and further demonstrated that imbalanced Lands’ cycle induced LysoPC production directly promotes sickling in cultured mouse and human SCD erythrocytes. Mechanistically, we revealed that hypoxia-mediated ERK activation underlies imbalanced Lands’ cycle by preferentially inducing the activity of PLA2 but not LPCAT in human and mouse SCD erythrocytes. Overall, our studies have identified a pathological role of imbalanced Lands’ cycle in SCD erythrocytes, novel molecular basis regulating Lands’ cycle and therapeutic opportunities for the disease.
Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43
Authors: Li Nan , Mruk Dolores D. , Chen Haiqi , Wong Chris K. C. , Lee Will M. , Cheng C. Yan 
Published: July 2016

AbstractPerfluorooctanesulfonate (PFOS) is an environmental toxicant used in developing countries, including China, as a stain repellent for clothing, carpets and draperies, but it has been banned in the U.S. and Canada since the late 2000s. PFOS perturbed the Sertoli cell tight junction (TJ)-permeability barrier, causing disruption of actin microfilaments in cell cytosol, perturbing the localization of cell junction proteins (e.g., occluden-ZO-1, N-cadherin-ß-catenin). These changes destabilized Sertoli cell blood-testis barrier (BTB) integrity. These findings suggest that human exposure to PFOS might induce BTB dysfunction and infertility. Interestingly, PFOS-induced Sertoli cell injury associated with a down-regulation of the gap junction (GJ) protein connexin43 (Cx43). We next investigated if overexpression of Cx43 in Sertoli cells could rescue the PFOS-induced cell injury. Indeed, overexpression of Cx43 in Sertoli cells with an established TJ-barrier blocked the disruption in PFOS-induced GJ-intercellular communication, resulting in the re-organization of actin microfilaments, which rendered them similar to those in control cells. Furthermore, cell adhesion proteins that utilized F-actin for attachment became properly distributed at the cell-cell interface, resealing the disrupted TJ-barrier. In summary, Cx43 is a good target that might be used to manage PFOS-induced reproductive dysfunction.
The association between types of regular primary care and hospitalization among people with and without multimorbidity: A household survey on 25,780 Chinese
Authors: Chung Roger Y. , Mercer Stewart W. , Yip Benjamin H. K. , Chan Stephanie W. C. , Lai Francisco T. T. , Wang Harry H. X. , Wong Martin C. S. , Wong Carmen K. M. , Sit Regina W. S. , Yeoh Eng-Kiong , Wong Samuel Y. S. 
Published: July 2016

AbstractUsing data collected from 25,780 Hong Kong citizens in a household survey, this study aimed to investigate the association between having regular source of primary care and hospitalization amongst people with and without multimorbidity (two or more chronic conditions). Potential interaction effects of regular primary care with multimorbidity were also examined. Results revealed a significant association between having regular source of primary care from General Practitioners and reduced hospitalization amongst respondents with multimorbidity (RR = 0.772; 95% CI = 0.667–0.894), adjusting for other potential confounding factors (i.e., socio-demographic factors and medical insurance and benefits). In contrast, having regular Specialist care was significantly associated with increased risk of hospitalization among both people with multimorbidity (RR = 1.619; 95% CI = 1.256–2.087) and without multimorbidity (RR = 1.981; 95% CI = 1.246–3.149), adjusting for potential confounders. A dose-response relationship between the number of chronic diseases and hospitalization was also observed, regardless of whether participants had regular source of primary care or not; relative risks and predicted probabilities for hospitalization were generally greater for those without regular source of primary care. Further studies are warranted to explore the role of healthcare system, informatics, organizational and practice-related factors on healthcare and functional outcomes.
Subject-Specific Effect of Metallic Body Accessories on Path Loss of Dynamic on-Body Propagation Channels
Authors: Rahim H. A. , Abdulmalek M. , Soh P. J. , Rani K. A. , Hisham N. , Vandenbosch G. A. E. 
Published: July 2016

AbstractThis paper presents the investigation of path loss variation for subject-specific on-body radio propagation channels, considering the effect of metallic spectacles and loop like metallic accessories. Adding metallic items may affect the operability of Body Centric Wireless Communications (BCWC). Measurements were carried out in an RF-shielded room lined with microwave absorbing sheets for strategically placed bodyworn antennas covering the upper front torso and the lower limbs. The path loss of the on-body radio channel was characterized explicitly taking into account the body size of the subjects. For metallic loop-like accessories, the results indicate that for underweight subjects, there was a slightly higher influence, up to 2%, compared to normal and overweight subjects. Our findings indicate that a noticeable effect exists on on-body channels for dynamic movements where the metallic watch acts as a local scatterer that affects the non-line-of-sight (NLOS) signal path between transmitter and receiver for underweight subjects in comparison to normal and overweight subjects. The path loss decreases when the receiving terminal was positioned very close to the metallic item. If a loop-like metallic accessory is not appropriately considered when designing the radio channel on a subject, the reliability of the body-centric wireless system may degrade.
Ethylene Response Factor TERF1, Regulated by ETHYLENE-INSENSITIVE3-like Factors, Functions in Reactive Oxygen Species (ROS) Scavenging in Tobacco (Nicotiana tabacum L.)
Authors: Zhang Hongbo , Li Ang , Zhang Zhijin , Huang Zejun , Lu Pingli , Zhang Dingyu , Liu Xinmin , Zhang Zhong-Feng , Huang Rongfeng 
Published: July 2016

AbstractThe phytohormone ethylene plays a crucial role in the production and accumulation of reactive oxygen species (ROS) in plants under stress conditions. Ethylene response factors (ERFs) are important ethylene-signaling regulators functioning in plant defense responses against biotic and abiotic stresses. However, the roles of ERFs during plant adapting to ROS stress have not yet been well documented. Our studies previously reported that a tomato ERF transcription factor TERF1 functions in the regulation of plant ethylene responses and stress tolerance. Here, we report our findings regarding the roles of TERF1 in ROS scavenging. In this study, we revealed that the transcription of TERF1 is regulated by upstream EIN3-like (EIN3, ethylene-insensitive 3) regulators LeEIL3 and LeEIL4 in tomato (Solanum lycopersicum) and is also inducible by exogenous applied ROS-generating reagents. Ectopic expression of TERF1 in tobacco promoted the expression of genes involved in oxidative stress responses, including carbonic anhydrase functioning in hypersensitive defense, catalase and glutathione peroxidase catalyzing oxidative reactions and GDP-D-mannose pyrophosphorylase functioning in ascorbic acid biosynthesis, reduced the ROS content induced by ethylene treatment and enhanced stress tolerance of tobacco seedlings to hydrogen peroxide (H2O2). Cumulatively, these findings suggest that TERF1 is an ethylene inducible factor regulating ROS scavenging during stress responses.
Seismic evidence for flow in the hydrated mantle wedge of the Ryukyu subduction zone
Authors: Nagaya Takayoshi , Walker Andrew M. , Wookey James , Wallis Simon R. , Ishii Kazuhiko , Kendall J. -Michael 
Published: July 2016

AbstractIt is widely accepted that water-rich serpentinite domains are commonly present in the mantle above shallow subducting slabs and play key roles in controlling the geochemical cycling and physical properties of subduction zones. Thermal and petrological models show the dominant serpentine mineral is antigorite. However, there is no good consensus on the amount, distribution and alignment of this mineral. Seismic velocities are commonly used to identify antigorite-rich domains, but antigorite is highly-anisotropic and depending on the seismic ray path, its properties can be very difficult to distinguish from non-hydrated olivine-rich mantle. Here, we utilize this anisotropy and show how an analysis of seismic anisotropy that incorporates measured ray path geometries in the Ryukyu arc can constrain the distribution, orientation and amount of antigorite. We find more than 54% of the wedge must consist of antigorite and the alignment must change from vertically aligned to parallel to the slab. This orientation change suggests convective flow in the hydrated forearc mantle. Shear wave splitting analysis in other subduction zones indicates large-scale serpentinization and forearc mantle convection are likely to be more widespread than generally recognized. The view that the forearc mantle of cold subduction zones is dry needs to be reassessed.
Auditory Speech Perception Development in Relation to Patient's Age with Cochlear Implant
Authors: Ciscare Grace , Mantello Erika , Fortunato-Queiroz Carla , Hyppolito Miguel , Reis Ana 
Published: July 2016

Introduction A cochlear implant in adolescent patients with pre-lingual deafness is still a debatable issue.
Objective The objective of this study is to analyze and compare the development of auditory speech perception in children with pre-lingual auditory impairment submitted to cochlear implant, in different age groups in the first year after implantation.
Method This is a retrospective study, documentary research, in which we analyzed 78 reports of children with severe bilateral sensorineural hearing loss, unilateral cochlear implant users of both sexes. They were divided into three groups: G1, 22 infants aged less than 42 months; G2, 28 infants aged between 43 to 83 months; and G3, 28 older than 84 months. We collected medical record data to characterize the patients, auditory thresholds with cochlear implants, assessment of speech perception, and auditory skills.
Results There was no statistical difference in the association of the results among groups G1, G2, and G3 with sex, caregiver education level, city of residence, and speech perception level. There was a moderate correlation between age and hearing aid use time, age and cochlear implants use time. There was a strong correlation between age and the age cochlear implants was performed, hearing aid use time and age CI was performed.
Conclusion There was no statistical difference in the speech perception in relation to the patient's age when cochlear implant was performed. There were statistically significant differences for the variables of auditory deprivation time between G3 - G1 and G2 - G1 and hearing aid use time between G3 - G2 and G3 - G1.
Cell division plane orientation based on tensile stress inArabidopsis thaliana
Authors: Louveaux Marion , Julien Jean-Daniel , Mirabet Vincent , Boudaoud Arezki , Hamant Olivier 
Published: July 2016

SignificanceThe control of cell division plane orientation is crucial in biology and most particularly in plants, in which cells cannot rearrange their positions, as they are glued to each other by their cell walls. Cell geometry has long been proposed to determine cell division plane orientation. Here, using statistical analysis, modeling, and live imaging in theArabidopsisshoot apex, we show that plant cells instead divide along maximal tension.
Transcriptional control of amino acid homeostasis is disrupted in Huntington’s disease
Authors: Sbodio Juan I. , Snyder Solomon H. , Paul Bindu D. 
Published: July 2016

Significance
Aberrant amino acid metabolism has been reported in Huntington’s disease (HD), but its molecular origins are unknown. We show here that the master regulator of amino acid homeostasis, activating transcription factor 4 (ATF4), is dysfunctional in HD, reflecting oxidative stress generated by impaired cysteine biosynthesis and transport. Accordingly, antioxidant supplementation reverses the diminished ATF4 response to nutrient stress. We identify a molecular link between amino acid disposition and oxidative stress that underlies multiple degenerative processes in HD. This disruption may be relevant to cellular dysfunction in other neurodegenerative conditions involving oxidative stress. Agents that restore cysteine balance may provide therapeutic benefit.
Spatially patterned matrix elasticity directs stem cell fate
Authors: Yang Chun , DelRio Frank W. , Ma Hao , Killaars Anouk R. , Basta Lena P. , Kyburz Kyle A. , Anseth Kristi S. 
Published: July 2016

Significance
The expansion and differentiation of human mesenchymal stem cells (hMSCs), an important cell type that is frequently used in stem-cell–based therapies, are regulated by interactions with their local extracellular microenvironment. Matrix stiffness is one of the key regulators, but often varies in space at a range of length scales in the local niche. To better understand and harness cell–matrix interactions, we used an innovative phototunable hydrogel platform to provide compelling evidence that the magnitude and spatial organization of matrix mechanics influence hMSCs through systematic regulation of cytoskeletal tension and transcriptional activation. These findings may have important implications in deepening our fundamental understanding of the role of extracellular matrix organization on disease, aging, and regenerative processes.
Predictive Validity of a Quality Measure for Intensive Substance use Disorder Treatment
Authors: Schmidt Eric M. , Gupta Shalini , Bowe Thomas , Ellerbe Laura S. , Phelps Tyler E. , Finney John W. , Asch Steven M. , Humphreys Keith , Trafton Jodie , Vanneman Megan , Harris Alex H. S. 
Published: July 2016

Background Measures of substance use disorder (SUD) treatment quality are essential tools for performance improvement. The Veterans Health Administration (VHA) developed a measure of access to and engagement in intensive outpatient programs (IOPs) for SUD. However, predictive validity, or associations between this measure and treatment outcomes, has not been examined. Methods Data on veterans with SUD came from 3 samples: the Outcomes Monitoring Project ( N = 5436), a national evaluation of VHA mental health services ( N = 339,887), and patients receiving detoxification services ( N = 23,572). Propensity score–weighted mixed-effects regressions modeled associations between receiving at least 1 week of IOP treatment and patient outcomes, controlling for facility-level performance and a random effect for facility. Results Propensity score weighting reduced or eliminated observable baseline differences between patient groups. Patients who accessed IOPs versus those who did not reported significantly reduced alcohol- and drug-related symptom severity, with significantly fewer past-month days drinking alcohol ( b = 1.83, P < .001) and fewer past-month days intoxicated ( b = 1.55, P < .001). Patients who received IOP after detoxification services had higher 6-month utilization of SUD outpatient visits ( b = 2.09, P < .001), more subsequent detoxification episodes ( b = 0.25, P < .001), and lower odds of 2-year mortality (odds ratio [OR] = 0.68, 95% confidence interval [CI]: 0.61–0.75; P < .001). Conclusions Receiving at least 1 week of SUD treatment in an IOP was associated with higher follow-up utilization, improved health outcomes, and reduced mortality. These associations lend support to the predictive validity of VHA's IOP quality measure. Future research should focus on measure feasibility and validity outside of VHA, and whether predictive validity is maintained once this quality measure is tied to performance incentives.
Magnetic structure and frequency scaling of limit-cycle oscillations close to L- to H-mode transitions
Authors: Birkenmeier G. , Cavedon M. , Conway G.D. , Manz P. , Stroth U. , Fischer R. , Fuchert G. , Happel T. , Laggner F.M. , Maraschek M. , Medvedeva A. , Nikolaeva V. , Prisiazhniuk D. , Pütterich T. , Ryter F. , Shao L.M. , Willensdorfer M. , Wolfrum E. , Zohm H. 
Published: July 2016

Abstract
Limit-cycle oscillations (LCOs) close to the power threshold of L- to H-mode transitions are investigated in plasmas of ASDEX Upgrade. During this phase, referred to as I-phase, a strong magnetic activity in the poloidal magnetic field

B
⋅

θ

with an up–down asymmetry is found. In some cases, the regular LCOs during I-phase transition smoothly into a phase with intermittent bursts which have similar properties to type-III edge localised modes (ELMs). Indications of precursors during the intermittent phase as well as in the regular LCO phase point to a common nature of the I-phase and type-III ELMs. The LCO frequency measured in a set of discharges with different plasma currents and magnetic fields scales as

f
∼
(

B

t

1

/

2

I

p

3

/

2

)

/

(
n
T
)

.
Sex-Differential Non-Vaccine-Specific Immunological Effects of Diphtheria-Tetanus-Pertussis and Measles Vaccination
Authors: Noho-Konteh Fatou , Adetifa Jane U. , Cox Momodou , Hossin Safayet , Reynolds John , Le My Thanh , Sanyang Lady Chilel , Drammeh Abdoulie , Plebanski Magdalena , Forster Thorsten , Dickinson Paul , Ghazal Peter , Whittle Hilton , Rowland-Jones Sarah L. , Sutherland Jayne S. , Flanagan Katie L. 
Published: July 2016

Abstract
Vaccines can have nontargeted heterologous effects that manifest as increased protection against nonvaccine infections, as described for measles vaccine (MV), or increased susceptibility to infections and death, as described following diphtheria-tetanus-whole cell pertussis (DTP) vaccination. The mechanisms are unknown, and high-quality immunological studies are lacking. This study was designed to investigate the heterologous effects of MV and DTP in 302 Gambian infants. The results support a sex-differential immunosuppressive effect of DTP on innate proinflammatory responses and T-cell immunity. Males but not females receiving MV had enhanced proinflammatory innate responses. The results point to modified signaling via Toll-like receptor 4 (TLR4) as a possible mechanism for the effects on innate immunity. When both vaccines were administered together, purified protein derivative responses were enhanced in females but downregulated in males. Collectively, these data indicate immunological effects that could account for heterologous effects of MV and DTP, to take forward into prospective trials.
High-energy gamma-ray observations of the accreting black hole V404 Cygni during its 2015 June outburst
Authors: Loh A. , Corbel S. , Dubus G. , Rodriguez J. , Grenier I. , Hovatta T. , Pearson T. , Readhead A. , Fender R. , Mooley K. 
Published: July 2016

Abstract
We report on Fermi/Large Area Telescope observations of the accreting black hole low-mass X-ray binary V404 Cygni during its outburst in 2015 June–July. Detailed analyses reveal a possible excess of γ-ray emission on 2015 26 June, with a very soft spectrum above 100 MeV, at a position consistent with the direction of V404 Cyg (within the 95 per cent confidence region and a chance probability of 4 × 10−4). This emission cannot be associated with any previously known Fermi source. Its temporal coincidence with the brightest radio and hard X-ray flare in the light curve of V404 Cyg, at the end of the main active phase of its outburst, strengthens the association with V404 Cyg. If the γ-ray emission is associated with V404 Cyg, the simultaneous detection of 511 keV annihilation emission by INTEGRAL reqires that the high-energy γ-rays originate away from the corona, possibly in a Blandford–Znajek jet. The data give support to models involving a magnetically arrested disc where a bright γ-ray jet can re-form after the occurrence of a major transient ejection seen in the radio.