bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns
Authors: Williamson Deborah A. , Carter Glen P. , Howden Benjamin P. 
Published: June 2017

SUMMARYBacterial skin infections represent some of the most common infectious diseases globally. Prevention and treatment of skin infections can involve application of a topical antimicrobial, which may be an antibiotic (such as mupirocin or fusidic acid) or an antiseptic (such as chlorhexidine or alcohol). However, there is limited evidence to support the widespread prophylactic or therapeutic use of topical agents. Challenges involved in the use of topical antimicrobials include increasing rates of bacterial resistance, local hypersensitivity reactions (particularly to older agents, such as bacitracin), and concerns about the indiscriminate use of antiseptics potentially coselecting for antibiotic resistance. We review the evidence for the major clinical uses of topical antibiotics and antiseptics. In addition, we review the mechanisms of action of common topical agents and define the clinical and molecular epidemiology of antimicrobial resistance in these agents. Moreover, we review the potential use of newer and emerging agents, such as retapamulin and ebselen, and discuss the role of antiseptic agents in preventing bacterial skin infections. A comprehensive understanding of the clinical efficacy and drivers of resistance to topical agents will inform the optimal use of these agents to preserve their activity in the future.
Validation and Implementation of Clinical Laboratory Improvements Act-Compliant Whole-Genome Sequencing in the Public Health Microbiology Laboratory
Authors: Kozyreva Varvara K. , Truong Chau-Linda , Greninger Alexander L. , Crandall John , Mukhopadhyay Rituparna , Chaturvedi Vishnu 
Published: June 2017

ABSTRACT

Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the validation of WGS on the Illumina platform for routine use in PHLs according to Clinical Laboratory Improvements Act (CLIA) guidelines for laboratory-developed tests (LDTs). We developed a validation panel comprising 10
Enterobacteriaceae
isolates, 5 Gram-positive cocci, 5 Gram-negative nonfermenting species, 9
Mycobacterium tuberculosis
isolates, and 5 miscellaneous bacteria. The genome coverage range was 15.71× to 216.4× (average, 79.72×; median, 71.55×); the limit of detection (LOD) for single nucleotide polymorphisms (SNPs) was 60×. The accuracy, reproducibility, and repeatability of base calling were >99.9%. The accuracy of phylogenetic analysis was 100%. The specificity and sensitivity inferred from multilocus sequence typing (MLST) and genome-wide SNP-based phylogenetic assays were 100%. The following objectives were accomplished: (i) the establishment of the performance specifications for WGS applications in PHLs according to CLIA guidelines, (ii) the development of quality assurance and quality control measures, (iii) the development of a reporting format for end users with or without WGS expertise, (iv) the availability of a validation set of microorganisms, and (v) the creation of a modular template for the validation of WGS processes in PHLs. The validation panel, sequencing analytics, and raw sequences could facilitate multilaboratory comparisons of WGS data. Additionally, the WGS performance specifications and modular template are adaptable for the validation of other platforms and reagent kits.
A case of a chronic salmonella infection following Roux-en-Y gastric bypass surgery, treated successfully by a laparoscopic cholecystectomy
Authors: Siddiqui Zohaib , Siddiqui Zain Ahmed , Husain Fahd , Siddiqui Midhat 
Published: June 2017

We report a case of chronic infection caused bySalmonellaand cured by a laparoscopic cholecystectomy after Roux-en-Y gastric bypass (RYGB) surgery for obesity. This patient presented with a 2-year history of chronic abdominal pain, loose stools and excessive weight loss. Her stool and urine cultures were positive forSalmonella. Despite multiple courses of antibiotics, she remained positive.After undergoing a laparoscopic cholecystectomy, the patient became asymptomatic and stools remained negative. In chronic carriers forSalmonella, the gall bladder is the common reservoir for the bacteria and removing it is usually curative.The possibility that the source of the may be in the biliary limb of her bariatric procedure and not in the gall bladder remained a concern.In patients who have had a RYGB, cholecystectomy is an effective treatment.All patients presenting with abdominal symptoms following RYGB should have stool and urine cultures taken as part of their work up.
Rome Foundation-Asian working team report: Asian functional gastrointestinal disorder symptom clusters
Authors: Siah Kewin Tien Ho , Gong Xiaorong , Yang Xi Jessie , Whitehead William E , Chen Minhu , Hou Xiaohua , Pratap Nitesh , Ghoshal Uday C , Syam Ari F , Abdullah Murdani , Choi Myung-Gyu , Bak Young-Tae , Lu Ching-Liang , Gonlachanvit Sutep , Boon Chua Seng , Fang Fan , Cheong Pui Kuan , Wu Justin C Y , Gwee Kok-Ann 
Published: June 2017

ObjectiveFunctional gastrointestinal disorders (FGIDs) are diagnosed by the presence of a characteristic set of symptoms. However, the current criteria-based diagnostic approach is to some extent subjective and largely derived from observations in English-speaking Western patients. We aimed to identify latent symptom clusters in Asian patients with FGID.Design1805 consecutive unselected patients with FGID who presented for primary or secondary care to 11 centres across Asia completed a cultural and linguistic adaptation of the Rome III Diagnostic Questionnaire that was translated to the local languages. Principal components factor analysis with varimax rotation was used to identify symptom clusters.ResultsNine symptom clusters were identified, consisting of two oesophageal factors (F6: globus, odynophagia and dysphagia; F9: chest pain and heartburn), two gastroduodenal factors (F5: bloating, fullness, belching and flatulence; F8 regurgitation, nausea and vomiting), three bowel factors (F2: abdominal pain and diarrhoea; F3: meal-related bowel symptoms; F7: upper abdominal pain and constipation) and two anorectal factors (F1: anorectal pain and constipation; F4: diarrhoea, urgency and incontinence).ConclusionWe found that the broad categorisation used both in clinical practice and in the Rome system, that is, broad anatomical divisions, and certain diagnoses with long historical records, that is, IBS with diarrhoea, and chronic constipation, are still valid in our Asian societies. In addition, we found a bowel symptom cluster with meal trigger and a gas cluster that suggests a different emphasis in our populations. Future studies to compare a non-Asian cohort and to match to putative pathophysiology will help to verify our findings.
Characteristics research of pressure sensor based on nanopolysilicon thin films resistors
Authors: Zhao Xiaofeng , Li Dandan , Wen Dianzhong 
Published: June 2017

To further improve the sensitivity temperature characteristics of pressure sensor, a kind of pressure sensor taking nanopolysilicon thin films as piezoresistors is proposed in this paper. On the basis of the microstructure analysis by X-ray diffraction (XRD) and scanning electron microscope (SEM) tests, the preparing process of nanopolysilicon thin films is optimized. The effects of film thickness and annealing temperature on the micro-structure of nanopolysilicon thin films were studied, respectively. In order to realize the measurement of external pressure, four nanopolysilicon thin films resistors were arranged at the edges of square silicon diaphragm connected to a Wheatstone bridge, and the chip of the sensor was designed and fabricated on a [Formula: see text] orientation silicon wafer by microelectromechanical system (MEMS) technology. Experimental result shows that when [Formula: see text] = 6.80 mA, the sensitivity of the sensor PS-1 is 0.308 mV/kPa, and the temperature coefficient of sensitivity (TCS) is about −1742 ppm/[Formula: see text]C in the range of −40–140[Formula: see text]C. It is possible to obviously improve the sensitivity temperature characteristics of pressure sensor by the proposed sensors.
Application of Euclidean distance mapping for assessment of basement membrane thickness distribution in asthma
Authors: Mostaco-Guidolin Leila , Hajimohammadi Soheil , Vasilescu Dragoş M. , Hackett Tillie-Louise 
Published: June 2017

Abnormal thickening of the airway basement membrane is one of the hallmarks of airway remodeling in asthma. The present protocols for measuring the basement membrane involve the use of stained tissue sections and measurements of the basement membrane thickness at certain intervals, followed by the calculation of the geometric mean thickness for each airway. This report describes an automated, unbiased approach which uses color segmentation to identify structures of interest on stained sections and Euclidean distance mapping to measure the thickness distribution of airway structures. This method was applied to study the thickness distribution of the basement membrane and airway epithelium in lungs donated for research from seven nonasthmatic and eight asthmatic age- and sex-matched donors. A total of 60 airways were assessed. We report that the thickness and thickness distribution of the basement membrane and airway epithelium are increased in large and small airways of asthmatics compared with nonasthmatics. Using this method we were able to demonstrate the heterogeneity in the thickness of the basement membrane and airway epithelium within individual airways of asthmatic subjects. This new computational method enables comprehensive and objective quantification of airway structures, which can be used to quantify heterogeneity of airway remodeling in obstructive lung diseases such as asthma and chronic obstructive pulmonary disease. NEW & NOTEWORTHY The described application of Euclidean distance mapping provides an unbiased approach to study the extent and thickness distribution of changes in tissue structures. This approach will enable researchers to use computer-aided analysis of structural changes within lung tissue to understand the heterogeneity of airway remodeling in lung diseases.
Timing of postexercise carbohydrate-protein supplementation: roles of gastrointestinal blood flow and mucosal cell damage on gastric emptying in humans
Authors: Kashima Hideaki , Harada Nao , Miyamoto Kanae , Fujimoto Masaki , Fujita Chiaki , Endo Masako Yamaoka , Kobayashi Toshio , Miura Akira , Fukuba Yoshiyuki 
Published: June 2017

It is well known that protein ingestion immediately after exercise greatly stimulates muscle protein synthesis during the postexercise recovery phase. However, immediately after strenuous exercise, the gastrointestinal (GI) mucosa is frequently injured by hypoperfusion in the organ/tissue, possibly resulting in impaired GI function (e.g., gastric emptying; GE). The aim of this study was to examine the effect of GI blood flow on the GE rate. Eight healthy young subjects performed an intermittent supramaximal cycling exercise for 30 min, which consisted of a 120% V̇o2peak for 20 s, followed by 20 W for 40 s. The subjects ingested 300 ml of a nutrient drink containing carbohydrate-protein at either 5 min postexercise in one trial (PE-5) or 30 min postexercise in another trial (PE-30). In the control trial (Con), the subjects ingested the same drink without exercise. The celiac artery blood flow (CABF) and superior mesenteric artery blood flow (SMABF) and GE rate were assessed by ultrasonography. Before drink ingestion in PE-5, CABF significantly decreased from baseline, whereas in PE-30, it returned to baseline. Following drink ingestion in PE-5, CABF did not change from baseline, but it significantly increased in PE-30 and Con. SMABF increased significantly later in PE-5 than in PE-30 and Con. The GE rate was consistently slower in PE-5 than in PE-30 and Con. In conclusion, the CABF response after exercise seems to modulate the subsequent GE rate and SMABF response. NEW & NOTEWORTHY A carbohydrate-protein drink was ingested at either 5 min (i.e., profoundly decreased celiac artery blood flow; CABF) or 30 min (i.e., already recovered CABF) postexercise. In the 5-min postexercise trial, the gastric emptying (GE) rate and superior mesenteric artery blood flow (SMABF) response were slower than those in the 30-min postexercise trial. The GE rate and SMABF response may be altered depending on the postexercise CABF response.
Venous thromboembolism: Past, present and future
Authors: Schulman Sam , Ageno Walter , Konstantinides Stavros V. 
Published: June 2017

SummaryVenous thromboembolism (VTE), the third most frequent acute cardiovascular syndrome, is associated with a considerable disease burden which continues to grow along with the longer life expectancy of the population worldwide. In the past century, parenteral heparin prophylaxis was established for hospitalised patients at elevated risk of VTE. More recently, non-vitamin K antagonist oral anticoagulants (NOACs) with a direct inhibiting effect on factor Xa or thrombin, underwent extensive testing in clinical trials and have been approved for patients undergoing hip or knee replacement. Clinical investigation is ongoing in further areas of thromboprophylaxis, including medical prophylaxis in patients and high-risk situations in the outpatient setting. The diagnostic approach to suspected VTE is now based on advanced imaging techniques and robust diagnostic algorithms which ensure high sensitivity and specificity. Nevertheless, the role of clinical, or pre-test, probability assessment remains crucial to avoid overdiagnosis and treatment errors. Advances in reperfusion strategies, along progressive establishment of the NOACs as the new standard of anticoagulation treatment, have simplified the management of VTE, improving outcomes and particularly safety. While new molecular targets for anticoagulation are being investigated in the quest to further reduce bleeding risk, adjusting the initial regimen to the patient’s risk and finding the optimal duration of anticoagulation after an index VTE event will be some of the top priorities in the years to come. Importantly, and in parallel to new drugs and technical advances in imaging, incentives such as hospital accreditation and funding based on evidence-based practice need to be implemented to increase guideline adherence.
Ten‐Year (2001–2011) Trends in the Incidence Rates and Short‐Term Outcomes of Early Versus Late Onset Cardiogenic Shock After Hospitalization for Acute Myocardial Infarction
Authors: Nguyen Hoa L. , Yarzebski Jorge , Lessard Darleen , Gore Joel M. , McManus David D. , Goldberg Robert J. 
Published: June 2017

Background

Cardiogenic shock (
CS
) is a serious complication of acute myocardial infarction, and the time of onset of
CS
has a potential role in influencing its prognosis. Limited contemporary data exist on this complication, however, especially from a population‐based perspective. Our study objectives were to describe decade‐long trends in the incidence, in‐hospital mortality, and factors associated with the development of
CS
in 3 temporal contexts: (1) before hospital arrival for acute myocardial infarction (prehospital
CS
); (2) within 24 hours of hospitalization (early
CS
); and (3) ≥24 hours after hospitalization (late
CS
).

Methods and Results

The study population consisted of 5782 patients with an acute myocardial infarction who were admitted to all 11 hospitals in central Massachusetts on a biennial basis between 2001 and 2011. The overall proportion of patients who developed
CS
was 5.2%. The proportion of patients with prehospital
CS
(1.6%) and late
CS
(1.5%) remained stable over time, whereas the proportion of patients with early
CS
declined from 2.2% in 2001–2003 to 1.2% in 2009–2011. In‐hospital mortality for prehospital
CS
increased from 38.9% in 2001–2003 to 53.6% in 2009–2011, whereas in‐hospital mortality for early and late
CS
decreased over time (35.9% and 64.7% in 2001–2003 to 15.8% and 39.1% in 2009–2011, respectively).

Conclusions

Development of prehospital and in‐hospital
CS
was associated with poor short‐term survival and the in‐hospital death rates among those with prehospital
CS
increased over time. Interventions focused on preventing or treating prehospital and late
CS
are needed to improve in‐hospital survival after acute myocardial infarction.
What Determines the Distribution of Shallow Convective Mass Flux through a Cloud Base?
Authors: Sakradzija Mirjana , Hohenegger Cathy 
Published: June 2017

Abstract
The distribution of cloud-base mass flux is studied using large-eddy simulations (LESs) of two reference cases: one representing conditions over the tropical ocean and another one representing midlatitude conditions over land. To examine what sets the difference between the two distributions, nine additional LES cases are set up as variations of the two reference cases. It is found that the total surface heat flux and its changes over the diurnal cycle do not influence the distribution shape. The latter is also not determined by the level of organization in the cloud field. It is instead determined by the ratio of the surface sensible heat flux to the latent heat flux, that is, the Bowen ratio B. This ratio sets the thermodynamic efficiency of the moist convective heat cycle, which determines the portion of the total surface heat flux that can be transformed into mechanical work of convection against mechanical dissipation. The thermodynamic moist heat cycle sets the average mass flux per cloud 〈m〉, and through 〈m〉 it also controls the shape of the distribution. An expression for 〈m〉 is derived based on the moist convective heat cycle and is evaluated against LES. This expression can be used in shallow cumulus parameterizations as a physical constraint on the mass flux distribution. The similarity between the mass flux and the cloud area distributions indicates that B also has a role in shaping the cloud area distribution, which could explain its different shapes and slopes observed in previous studies.
The Subtropical Stratocumulus-Topped Planetary Boundary Layer: A Climatology and the Lagrangian Evolution
Authors: Eastman Ryan , Wood Robert , Ting O Kuan 
Published: June 2017

Abstract
Prior work has shown that deeper planetary boundary layers (PBLs) are associated with cloud breakup and reduced droplet concentration in subtropical stratocumulus cloud decks, motivating a need for a thorough understanding of PBL mechanics. Here, 169 000 boundary layer trajectories are calculated in four eastern subtropical ocean basins following reanalysis winds at 925 mb (1 mb = 1 hPa). These trajectories combined with a twice-daily cloud-top-height-inferred PBL depth product allow for a comprehensive Lagrangian analysis of the stratocumulus (Sc)-topped PBL as the cloud deck transitions from Sc to trade cumulus (Cu). Month-to-month variations of this PBL product are strongly positively correlated with an independent PBL product derived from GPS radio occultation.
A climatology shows the PBL deepening offshore in every region. The yearly cycle of PBL depth varies in opposition to the yearly cycle of lower-tropospheric stability (LTS), but high-frequency variation between LTS and PBL depth is more complex. Observed geographical patterns of Lagrangian PBL deepening rates appear nonuniform between and within study regions, with smaller regions of maximum deepening rates. A Lagrangian analysis suggests that many variables act to alter the PBL: increased sea surface temperature and droplet concentration act to deepen the PBL, while increases in upper-level humidity, LTS, precipitation, upper-level temperature, and subsidence lead to PBL shallowing.
A Sensitivity Study of an Effective Aerodynamic Parameter Scheme in Simulating Land–Atmosphere Interaction for a Sea–Land Breeze Case Around the Bohai Gulf of China
Authors: Zhong Zhong , Sun Yuan , Yang Xiu-Qun , Guo Weidong , Chen Haishan 
Published: June 2017

Abstract
Numerical simulations of the atmospheric boundary layer require careful representation of the surface heterogeneity, which involves the upscaling parameterization scheme for the heterogeneous surface parameters. In this study, the sensitivity comparisons of an effective aerodynamic parameter scheme against the area-weighted average scheme in simulating the land–atmosphere interaction over heterogeneous terrain were carried out by conducting multinested simulations with the Weather Research and Forecasting (WRF) Model at coarse and fine resolutions, for a typical sea–land breeze case in the Bohai Gulf of China. The results show that the limited-area model is sensitive to the aerodynamic parameter scheme and the effective aerodynamic parameter scheme exhibits a better performance in simulating the variables and parameters in the land–atmosphere interaction process, such as surface wind speed, sensible heat flux, latent heat flux, friction velocity, and surface air temperature, among others, for short-term simulations. Particularly, the underestimation of sensible heat flux and overestimation of latent heat flux over heterogeneous terrain with area-weighted average scheme for aerodynamic parameters can be improved with the effective parameter scheme in the coastal regions, where the mean simulation error with the effective parameter scheme is about one-half of that with the average scheme for sensible heat flux and one-third for latent heat flux.
Measurements of the Directional Spectrum across the Equilibrium Saturation Ranges of Wind-Generated Surface Waves
Authors: Lenain Luc , Melville W. Kendall 
Published: June 2017

AbstractIt is now well accepted that to better understand the coupling between the atmosphere and the ocean, and improve coupled ocean–atmosphere models, surface wave processes need to be taken into account. Here, properties of the directional distributions of the surface wave field across the equilibrium and saturation ranges are investigated from airborne lidar data collected during the ONR Southern California 2013 (SoCal2013) experiment, conducted off the coast of Southern California in November 2013. During the field effort, detailed characterization of the marine atmospheric boundary layer was performed from Research Platform (R/P) Floating Instrument Platform (FLIP), moored at the center of the aircraft operational domain. The wind speed ranged from approximately 1–2 to up to 11 m s−1, while the significant wave height varied from 0.8 to 2.5 m during the 10 days of data collection considered in the analysis. The directional wavenumber spectrum exhibits a clear, bimodal distribution that extends well beyond what was reported in previous studies, with the azimuthal separation between the lobes reaching ≈π for the highest wavenumbers that could be resolved: approximately 10–12 rad m−1. The results demonstrate that opposing wave components can be found in one storm system rather than requiring waves from opposing storms, with implications for ocean acoustics. With the broad wavenumber range of the directional spectra obtained from the lidar, the transition from the equilibrium to saturation ranges over a range of wind forcing conditions is found to occur for ≈ 1–2 × 10−3, where kn is the wavenumber at the upper limit of the equilibrium range, u* the friction velocity, and g the gravitational acceleration. The results are discussed in the context of Phillips’ model of the equilibrium range of wind-generated gravity waves.