bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aldosterone and Vascular Mineralocorticoid Receptors in Murine Endotoxic and Human Septic Shock*
Authors: Fadel Fouad , André-Grégoire Gwennan , Gravez Basile , Bauvois Brigitte , Bouchet Sandrine , Sierra-Ramos Catalina , Polito Andrea , Mansart Arnaud , Alvarez de la Rosa Diego , Annane Djillali , Jaisser Frédéric 
Published: April 2017

Objectives:
Vascular mineralocorticoid receptors play a role in vascular tone and blood pressure regulation, might participate in the pathophysiology of circulatory failure during sepsis, and represent a potential therapeutic target in this disease. We aimed to study the effects of mineralocorticoids and the involvement of vascular mineralocorticoid receptors in murine endotoxic and human septic shock.

Design:
Experimental study.

Setting:
Translational investigation including animal research and in vitro experiments using human vascular cells and plasma from septic patients.

Subjects:
Adult male C57Black 6 mice, adult patients with septic shock.

Interventions:
Mice were injected with lipopolysaccharide and/or aldosterone. Human endothelial and smooth muscle cells were treated with pro-inflammatory cytokines with or without aldosterone, nuclear factor-κB inhibitor BAY 11-7082, or plasma from septic patients.

Measurements and Main Results:
Aldosterone improved 5-day survival, invasive arterial pressure, and in vivo and ex vivo arterial response to phenylephrine at 18 hours after induction of murine endotoxic shock. Both α1-adrenoceptor and mineralocorticoid receptor expressions studied in mouse aortas were down-regulated at 6 and 18 hours in endotoxemic mice and restored in aldosterone-treated mice. Furthermore, tumor necrosis factor-α decreased both mineralocorticoid receptor and α1-adrenoceptor expressions within 5 hours in human vascular cells in a nuclear factor-κB pathway–dependent manner. Mineralocorticoid receptor expression was also blunted in human cells treated with plasma from septic patients.

Conclusion:
We found a beneficial effect of mineralocorticoids on survival, blood pressure, and vascular reactivity, associated with a restoration of α1-adrenoceptor expression in endotoxic shock. Furthermore, blunted vascular mineralocorticoid receptor expression might participate in hemodynamic failure during sepsis.
Plastic and Reconstructive Surgery in Global Health: Let’s Reconstruct Global Surgery
Authors: Chung Karen Y. 
Published: April 2017

Summary:
Since the inception of the Lancet Commission in 2013 and consequent prioritization of Global Surgery at the World Health Assembly, international surgical outreach efforts have increased and become more synergistic. Plastic surgeons have been involved in international outreach for decades, and there is now a demand to collaborate and address local need in an innovative way. The aim of this article was to summarize new developments in plastic and reconstructive surgery in global health, to unify our approach to international outreach. Specifically, 5 topics are explored: current models in international outreach, benefits and concerns, the value of research, the value of international surgical outreach education, and the value of technology. A “Let’s Reconstruct Global Surgery” network has been formed using Facebook as a platform to unite plastic and reconstructive surgeons worldwide who are interested in international outreach. The article concludes with actionable recommendations from each topic.
Amnion Membrane in Diabetic Foot Wounds: A Meta-analysis
Authors: Haugh Alexandra M. , Witt Jacqueline G. , Hauch Adam , Darden Michael , Parker Geoffrey , Ellsworth Warren A. , Buell Joseph F. 
Published: April 2017

Background:
Amniotic membrane is tissue obtained from human placenta rich in cytokines, growth factors, and stem cells that possess the ability to inhibit infection, improve healing, and stimulate regeneration.

Methods:
A meta-analysis was performed examining randomized controlled trials comparing amniotic tissue products with standard of care in nonhealing diabetic foot ulcers including PubMed, Cochrane Central Register of Controlled Trials, and the Cochrane Database of Systematic Reviews.

Results:
A search of 3 databases identified 596 potentially relevant articles. Application of selection criteria led to the selection of 5 randomized controlled trials. The 5 selected randomized controlled trials represented a total of 311 patients. The pooled relative risk of healing with amniotic products compared with control was 2.7496 (2.05725–3.66524, P < 0.001).

Conclusions:
The current meta-analysis indicates that the treatment of diabetic foot ulcers with amniotic membrane improves healing rates in diabetic foot ulcers. Further studies are needed to determine whether these products also decrease the incidence of subsequent complications, such as amputation or death, in diabetic patients.
One-Year Clinical Outcomes of a Corneal Inlay for Presbyopia
Authors: Beer Sandra M. C. , Santos Rodrigo , Nakano Eliane M. , Hirai Flavio , Nitschke Enrico J. , Francesconi Claudia , Campos Mauro 
Published: April 2017

Purpose:
To report the results of a 1-year follow-up analysis of the safety and efficacy of the Flexivue Microlens corneal inlay.

Methods:
The Flexivue Microlens corneal inlay was implanted in the nondominant eye of patients with emmetropic presbyopia (a spherical equivalent of −0.5 to 1.00 diopter) after the creation of a 300-μm deep stromal pocket, using a femtosecond laser. The patients were followed up according to a clinical protocol involving refraction, anterior segment imaging analysis (Oculyzer), and optical quality analysis (OPD-Scan).

Results:
Thirty-one patients were enrolled in this ongoing study. The mean age was 50.7 years (range 45–60 yrs), and 70% of the patients were female. The mean uncorrected near visual acuity improved to Jaeger 1 in 87.1% of the eyes treated with the inlays. All eyes improved 4 lines at all visits. The binocular uncorrected distance visual acuity was 20/20 in all patients. Ninety percent of the patients reported that their near vision was good or excellent. Some of the patients (16.1%) lost more than 3 lines of corrected distance visual acuity. At the latest follow-up, induction of a corneal spherical aberration to improve near visual acuity was statistically significant. No other intraoperative or postoperative complication was noted.

Conclusions:
The Flexivue Microlens provided patients with improved near vision, as a result of a negative spherical aberration. A significant loss of corrected distance visual acuity in the operated eyes was observed. Overall, this intracorneal inlay was an effective alternative to other procedures for the correction of presbyopia.
Increased Mortality and Length of Stay Associated With Medical Emergency Team Review in Hospitalized Pediatric Patients: A Retrospective Cohort Study*
Authors: McKelvie Brianna , McNally James Dayre , Chan Jason , Momoli Franco , Ramsay Christa , Lobos Anna-Theresa 
Published: April 2017

Objective:
Rapid response systems using medical emergency teams reduce hospital wide cardiorespiratory arrest and mortality. While rapid response systems improve hospital-wide outcomes, children receiving medical emergency team review may still be at increased risk for morbidity and mortality. The study purpose was to compare the length of stay and mortality rate in children receiving a medical emergency team review with those of other hospitalized children.

Design:
Retrospective cohort study.

Setting:
Tertiary Pediatric Hospital, Children’s Hospital of Eastern Ontario, Ottawa, Canada.

Patients:
Cohort of 42,308 pediatric admissions to the general inpatient ward.

Interventions:
Data over 7 years were obtained from a prospectively maintained rapid response systems database.

Measurements and Main Results:
From the cohort, 995 (2.35%) of the admissions had one and 276 (0.65%) had multiple medical emergency team activations. When compared with patients without, children having one or multiple medical emergency team reviews had 13.34 (95% CI, 5.33–33.2) and 50.10 (95% CI, 19.86–126.39) times the odds of death, respectively. Patients experiencing a medical emergency team review stayed in hospital 1.59 times (95% CI, 1.39–1.82) longer, whereas those with multiple medical emergency team reviews stayed 2.44 times (95% CI, 1.85–3.20) longer. The associations remained significant after controlling for important confounders and excluding elective admissions from the analyses. Most repeat medical emergency team reviews occurred within a day of the initial review or involved patients with multiple comorbidities.

Conclusions:
Our study suggests that pediatric patients reviewed by the medical emergency team are at significantly higher risk of mortality and longer length of stay than general ward inpatients. As well, patients with multiple medical emergency team reviews were at particularly high risk compared with patients with one medical emergency team review. Patients who experience medical emergency team reviews should be recognized as a high-risk group, and future studies should consider how to decrease morbidity and mortality. Based on our findings, we suggest that these patients be followed for 24–48 hours after any medical emergency team activation.
Accelerated aging in adults with knee osteoarthritis pain: consideration for frequency, intensity, time, and total pain sites
Authors: Sibille Kimberly T. , Chen Huaihou , Bartley Emily J. , Riley Joseph , Glover Toni L. , King Christopher D. , Zhang Hang , Cruz-Almeida Yenisel , Goodin Burel R. , Sotolongo Adriana , Petrov Megan E. , Herbert Matthew , Bulls Hailey W. , Edberg Jeffrey C. , Staud Roland , Redden David , Bradley Laurence A. , Fillingim Roger B. 
Published: April 2017

AbstractIntroduction:Individuals with osteoarthritis (OA) show increased morbidity and mortality. Telomere length, a measure of cellular aging, predicts increased morbidity and mortality. Telomeres shorten with persisting biological and psychosocial stress. Living with chronic OA pain is stressful. Previous research exploring telomere length in people with OA has produced inconsistent results. Considering pain severity may clarify the relationship between OA and telomeres.Objectives:We hypothesized that individuals with high OA chronic pain severity would have shorter telomeres than those with no or low chronic pain severity.Methods:One hundred thirty-six adults, ages 45 to 85 years old, with and without symptomatic knee OA were included in the analysis. Peripheral blood leukocyte telomere length was measured, and demographic, clinical, and functional data were collected. Participants were categorized into 5 pain severity groups based on an additive index of frequency, intensity, time or duration, and total number of pain sites (FITT). Covariates included age, sex, race or ethnicity, study site, and knee pain status.Results:The no or low chronic pain severity group had significantly longer telomeres compared with the high pain severity group,P= 0.025. A significant chronic pain severity dose response emerged for telomere length,P= 0.034. The FITT chronic pain severity index was highly correlated with the clinical and functional OA pain measures. However, individual clinical and functional measures were not associated with telomere length.Conclusion:Results demonstrate accelerated cellular aging with high knee OA chronic pain severity and provide evidence for the potential utility of the FITT chronic pain severity index in capturing the biological burden of chronic pain.
Tissue-specific downregulation ofEDTPsuppresses polyglutamine protein aggregates and extends lifespan inDrosophila
Authors: Xiao Chengfeng , Qiu Shuang , Robertson R Meldrum , Seroude Laurent 
Published: April 2017

ABSTRACTDrosophilaegg-derived tyrosine phosphatase (EDTP, also called JUMPY) is a lipid phosphatase essential in oogenesis and muscle function in the adult stage. Although mammalian JUMPY negatively regulates autophagy, loss-of-JUMPY causes muscle dysfunction and is associated with a rare genetic disorder called centronuclear myopathy. Here we show that tissue-specific downregulation of EDTP inDrosophilanon-muscle tissues, particularly glial cells, suppresses the expression of polyglutamine (polyQ) protein aggregates in the same cells and improves survival. Additionally, tissue-specific downregulation of EDTP in glial cells or motoneurons extends lifespan. We demonstrate an approach to fine-tune the expression of a disease-associated geneEDTPfor the removal of polyQ protein aggregate and lifespan extension inDrosophila.
Assessing species biomass contributions in microbial communities via metaproteomics
Authors: Kleiner Manuel , Thorson Erin , Sharp Christine E. , Dong Xiaoli , Liu Dan , Li Carmen , Strous Marc 
Published: April 2017

AbstractAssessment of microbial community composition is the cornerstone of microbial ecology. Microbial community composition can be analyzed by quantifying cell numbers or by quantifying biomass for individual populations. However, as cell volumes can differ by orders of magnitude, these two approaches yield vastly different results. Methods for quantifying cell numbers are already available (e.g. fluorescencein situhybridization, 16S rRNA gene amplicon sequencing), yet methods for assessing community composition in terms of biomass are lacking.We developed metaproteomics based methods for assessing microbial community composition using protein abundance as a measure for biomass contributions of individual populations. We optimized the accuracy and sensitivity of the method using artificially assembled microbial communities and found that it is less prone to some of the biases found in sequencing-based methods. We applied the method using communities from two different environments, microbial mats from two alkaline soda lakes and saliva from multiple individuals.
Identification ofcis-regulatory mutations generatingde novoedges in personalized cancer gene regulatory networks
Authors: Atak Zeynep Kalender , Imrichova Hana , Svetlichnyy Dmitry , Hulselmans Gert , Christiaens Valerie , Reumers Joke , Ceulemans Hugo , Aerts Stein 
Published: April 2017

AbstractThe identification of functional non-coding mutations is a key challenge in the field of genomics, where whole-genome re-sequencing can swiftly generate a set of all genomic variants in a sample, such as a tumor biopsy. The size of the human regulatory landscape places a challenge on finding recurrentcis-regulatory mutations across samples of the same cancer type. Therefore, powerful computational approaches are required to sift through the tens of thousands of non-coding variants, to identify potentially functional variants that have an impact on the gene expression profile of the sample. Here we introduce an integrative analysis pipeline, calledμ- cisTarget, to filter, annotate and prioritize non-coding variants based on their putative effect on the underlying 'personal' gene regulatory network. We first validateμ-cisTarget by re-analyzing three cases of oncogenic non-coding mutations, namely theTAL1andLMO1enhancer mutations in T-ALL, and theTERTpromoter mutation in melanoma. Next, we re-sequenced the full genome of ten cancer cell lines of six different cancer types, and used matched transcriptome data and motif discovery to infer master regulators for each sample. We identified candidate functional non-coding mutations that generatede novobinding sites for these master regulators, and that result in the up-regulation of nearby oncogenic drivers. We finally validated the predictions using tertiary data including matched epigenome data. Our approach is generally applicable to re-sequenced cancer genomes, or other genomes, when a disease- or sample-specific gene signature is available for network inference.μ-cisTarget is available fromhttp://mucistarget.aertslab.org.
MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation
Authors: Moreno-Fortuny Artal , Bragg Laricia , Cossu Giulio , Roostalu Urmas 
Published: April 2017

ABSTRACTCell polarity has a fundamental role in shaping the morphology of cells and growing tissues. Polarity is commonly thought to be established in response to extracellular signals. Here we used a minimalin vitroassay that enabled us to monitor the determination of cell polarity in myogenic and chondrogenic differentiation in the absence of external signalling gradients. We demonstrate that the initiation of cell polarity is regulated by melanoma cell adhesion molecule (MCAM). We found highly polarized localization of MCAM, Moesin (MSN), Scribble (SCRIB) and Van-Gogh-like 2 (VANGL2) at the distal end of elongating myotubes. Knockout of MCAM or elimination of its endocytosis motif does not impair the initiation of myogenesis or myoblast fusion, but prevents myotube elongation. MSN, SCRIB and VANGL2 remain uniformly distributed in MCAM knockout cells. We show that MCAM is also required at early stages of chondrogenic differentiation. In both myogenic and chondrogenic differentiation MCAM knockout leads to transcriptional downregulation ofScriband enhanced MAP kinase activity. Our data demonstrates the importance of cell autonomous polarity in differentiation.
Real-time 3D work-piece tracking with monocular camera based on static and dynamic model libraries
Authors: Zhu Wenjun , Wang Peng , Li Rui , Nie Xiangli 
Published: April 2017

Purpose
This paper aims to propose a novel real-time three-dimensional (3D) model-based work-piece tracking method with monocular camera for high-precision assembly. Tracking of 3D work-pieces with real-time speed is becoming more and more important for some industrial tasks, such as work-pieces grasping and assembly, especially in complex environment.

Design/methodology/approach
A three-step process method was provided, i.e. the offline static global library generation process, the online dynamic local library updating and selection process and the 3D work-piece localization process. In the offline static global library generation process, the computer-aided design models of the work-piece are used to generate a set of discrete two-dimensional (2D) hierarchical views matching libraries. In the online dynamic library updating and selection process, the previous 3D location information of the work-piece is used to predict the following location range, and a discrete matching library with a small number of 2D hierarchical views is selected from dynamic local library for localization. Then, the work-piece is localized with high-precision and real-time speed in the 3D work-piece localization process.

Findings
The method is suitable for the texture-less work-pieces in industrial applications.

Originality/value
The small range of the library enables a real-time matching. Experimental results demonstrate the high accuracy and high efficiency of the proposed method.
An assembly sensitivity analysis method based on state space model
Authors: Li Xin , Shang Jianzhong , Zhu Hong 
Published: April 2017

Purpose
This paper aims to consider a problem of assembly sensitivity in a multi-station assembly process. The authors focus on the assembly process of aircrafts, which includes cabins and inertial navigation system (INSs), and establish the assembly process state space model for their assembly sensitivity research.

Design/methodology/approach
To date, the process-related errors that cause large variations in key product characteristics remains one of the most critical research topics in assembly sensitivity analysis. This paper focuses on the unique challenges brought about by the multi-station system: a system-level model for characterizing the variation propagation in the entire process, and the necessity of describing the system response to variation inputs at both station-level and single fixture-level scales. State space representation is used to describe the propagation of variation in such a multi-station process, incorporating assembly process parameters such as fixture-locating layout at individual stations and station-to-station locating layout change.

Findings
Following the sensitivity analysis in control theory, a group of hierarchical sensitivity indices is defined and expressed in terms of the system matrices in the state space model, which are determined by the given assembly process parameters.

Originality/value
A case study of assembly sensitivity for a multi-station assembly process illustrates and validates the proposed methodology.
A hybrid Ant-Wolf Algorithm to optimize assembly sequence planning problem
Authors: Ab Rashid Mohd Fadzil Faisae 
Published: April 2017

Purpose
This paper aims to optimize the assembly sequence planning (ASP) problem using a proposed hybrid algorithm based on Ant Colony Optimization (ACO) and Gray Wolf Optimizer (GWO). The proposed Hybrid Ant-Wolf Algorithm (HAWA) is designed to overcome premature convergence in ACO.

Design/methodology/approach
The ASP problem is formulated by using task-based representation. The HAWA adopts a global pheromone-updating procedure using the leadership hierarchy concept from the GWO into the ACO to enhance the algorithm performance. In GWO, three leaders are assigned to guide the search direction, instead of a single leader in most of the metaheuristic algorithms. Three assembly case studies used to test the algorithm performance.

Findings
The proposed HAWA performed better in comparison to the Genetic Algorithm, ACO and GWO because of the balance between exploration and exploitation. The best solution guides the search direction, while the neighboring solutions from leadership hierarchy concept avoid the algorithm trapped in a local optimum.

Originality/value
The originality of this research is on the proposed HAWA. In addition to the standard pheromone-updating procedure, a global pheromone-updating procedure is introduced, which adopted leadership hierarchy concept from GWO.
A critical perspective on the measurement of performance in the empirical multinationality and performance literature
Authors: Richter Nicole Franziska , Schmidt Robert , Ladwig Tina Jessica , Wulhorst Fabian 
Published: April 2017

PurposeThis paper aims to contribute to the core research in international business (IB), namely, the relationship between multinationality and performance and is concerned with the quality of past empirical research designs.Design/methodology/approachOn the basis of 49 studies, given in a literature review, the match between performance measures used in empirical studies and the underlying theoretical streams that explain the effects on benefits and costs of multinationality is critically evaluated.FindingsFindings indicate that authors still largely rely on overall financial performance measures. Theoretical arguments, in contrast, refer to specific benefit and cost positions that might be better reflected in operational performance indicators. The idiosyncratic choice of the performance measures used might contribute to the varying results in past studies.Originality/valueSuggestions for improving future research designs are offered.