bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetrical Deterministic Lateral Displacement Gaps for Dual Functions of Enhanced Separation and Throughput of Red Blood Cells
Authors: Zeming Kerwin Kwek , Salafi Thoriq , Chen Chia-Hung , Zhang Yong 
Published: March 2016

AbstractDeterministic lateral displacement (DLD) method for particle separation in microfluidic devices has been extensively used for particle separation in recent years due to its high resolution and robust separation. DLD has shown versatility for a wide spectrum of applications for sorting of micro particles such as parasites, blood cells to bacteria and DNA. DLD model is designed for spherical particles and efficient separation of blood cells is challenging due to non-uniform shape and size. Moreover, separation in sub-micron regime requires the gap size of DLD systems to be reduced which exponentially increases the device resistance, resulting in greatly reduced throughput. This paper shows how simple application of asymmetrical DLD gap-size by changing the ratio of lateral-gap (GL) to downstream-gap (GD) enables efficient separation of RBCs without greatly restricting throughput. This method reduces the need for challenging fabrication of DLD pillars and provides new insight to the current DLD model. The separation shows an increase in DLD critical diameter resolution (separate smaller particles) and increase selectivity for non-spherical RBCs. The RBCs separate better as compared to standard DLD model with symmetrical gap sizes. This method can be applied to separate non-spherical bacteria or sub-micron particles to enhance throughput and DLD resolution.
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus
Authors: Wang Kun , Lu Wenxin , Tu Qichao , Ge Yichen , He Jinzhi , Zhou Yu , Gou Yaping , Van Nostrand Joy D , Qin Yujia , Li Jiyao , Zhou Jizhong , Li Yan , Xiao Liying , Zhou Xuedong 
Published: March 2016

AbstractSeveral studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated with OLP from saliva samples of two subtypes (reticular and erosive) of OLP patients and healthy controls. Our analyses indicated that the overall structure of the salivary microbiome was not significantly affected by disease status. However, we did observe evident variations in abundance for several taxonomic groups in OLP. Porphyromonas and Solobacterium showed significantly higher relative abundances, whereas Haemophilus, Corynebacterium, Cellulosimicrobium and Campylobacter showed lower abundances in OLP patients, as compared with healthy controls. In addition, we explored specific microbial co-occurrence patterns in OLP and revealed significantly fewer linkers of Streptococcus comprising species in erosive OLP. Furthermore, the disease severity and immune dysregulation were also genus-associated, including with Porphyromonas that correlated to disease scores and salivary levels of interleukin (IL)-17 and IL-23. Overall, this study provides a general description of oral microbiome in OLP and it will be useful for further investigation of their potential roles in the initiation and immune modulation of OLP.
Large-scale production of functional human lysozyme from marker-free transgenic cloned cows
Authors: Lu Dan , Liu Shen , Ding Fangrong , Wang Haiping , Li Jing , Li Ling , Dai Yunping , Li Ning 
Published: March 2016

AbstractHuman lysozyme is an important natural non-specific immune protein that is highly expressed in breast milk and participates in the immune response of infants against bacterial and viral infections. Considering the medicinal value and market demand for human lysozyme, an animal model for large-scale production of recombinant human lysozyme (rhLZ) is needed. In this study, we generated transgenic cloned cows with the marker-free vector pBAC-hLF-hLZ, which was shown to efficiently express rhLZ in cow milk. Seven transgenic cloned cows, identified by polymerase chain reaction, Southern blot and western blot analyses, produced rhLZ in milk at concentrations of up to 3149.19 ± 24.80 mg/L. The purified rhLZ had a similar molecular weight and enzymatic activity as wild-type human lysozyme possessed the same C-terminal and N-terminal amino acid sequences. The preliminary results from the milk yield and milk compositions from a naturally lactating transgenic cloned cow 0906 were also tested. These results provide a solid foundation for the large-scale production of rhLZ in the future.
Brain mechanisms associated with internally directed attention and self-generated thought
Authors: Benedek Mathias , Jauk Emanuel , Beaty Roger E. , Fink Andreas , Koschutnig Karl , Neubauer Aljoscha C. 
Published: March 2016

AbstractInternal cognition like imagination and prospection require sustained internally directed attention and involve self-generated thought. This fMRI study aimed to disentangle the brain mechanisms associated with attention-specific and task-specific processes during internally directed cognition. The direction of attention was manipulated by either keeping a relevant stimulus visible throughout the task, or by masking it, so that the task had to be performed “in the mind’s eye”. The level of self-directed thought was additionally varied between a convergent and a divergent thinking task. Internally directed attention was associated with increased activation in the right anterior inferior parietal lobe (aIPL), bilateral lingual gyrus and the cuneus, as well as with extended deactivations of superior parietal and occipital regions representing parts of the dorsal attention network. The right aIPL further showed increased connectivity with occipital regions suggesting an active top-down mechanism for shielding ongoing internal processes from potentially distracting sensory stimulation in terms of perceptual decoupling. Activation of the default network was not related to internally directed attention per se, but rather to a higher level of self-generated thought. The findings hence shed further light on the roles of inferior and superior parietal cortex for internally directed cognition.
Endoscopic ultrasound-guided fine needle core biopsy for the diagnosis of pancreatic malignant lesions: a systematic review and Meta-Analysis
Authors: Yang Yongtao , Li Lianyong , Qu Changmin , Liang Shuwen , Zeng Bolun , Luo Zhiwen 
Published: March 2016

AbstractEndoscopic ultrasound-guided fine needle core biopsy (EUS-FNB) has been used as an effective method of diagnosing pancreatic malignant lesions. It has the advantage of providing well preserved tissue for histologic grading and subsequent molecular biological analysis. In order to estimate the diagnostic accuracy of EUS-FNB for pancreatic malignant lesions, studies assessing EUS-FNB to diagnose solid pancreatic masses were selected via Medline. Sixteen articles published between 2005 and 2015, covering 828 patients, met the inclusion criteria. The summary estimates for EUS-FNB differentiating malignant from benign solid pancreatic masses were: sensitivity 0.84 (95% confidence interval (CI), 0.82–0.87); specificity 0.98 (95% CI, 0.93–1.00); positive likelihood ratio 8.0 (95% CI 4.5–14.4); negative likelihood ratio 0.17 (95% CI 0.10–0.26); and DOR 64 (95% CI 30.4–134.8). The area under the sROC curve was 0.96. Subgroup analysis did not identify other factors that could substantially affect the diagnostic accuracy, such as the study design, location of study, number of centers, location of lesion, whether or not a cytopathologist was present and so on. EUS-FNB is a reliable diagnostic tool for solid pancreatic masses and should be especially considered for pathology where histologic morphology is preferred for diagnosis.
Human activity accelerating the rapid desertification of the Mu Us Sandy Lands, North China
Authors: Miao Yunfa , Jin Heling , Cui Jianxin 
Published: March 2016

AbstractOver the past several thousand years, arid and semiarid China has experienced a series of asynchronous desertification events in its semiarid sandy and desert regions, but the precise identification of the driving forces of such events has remained elusive. In this paper we identify two rapid desertification events (RDEs) at ~4.6 ± 0.2 ka BP and ~3.3 ± 0.2 ka BP from the JJ Profile, located in the eastern Mu Us Sandy Lands. These RDEs appear to have occurred immediately following periods marked by persistently frequent and intense fires. We argue that such fire patterns, directly linked to an uncontrolled human use of vegetation as fuel, played a key role in accelerating RDEs by ensuring that the land surface was degraded beyond the threshold required for rapid desertification. This would suggest that the future use of a massive and sustained ecological program of vegetation rehabilitation should reduce the risk of destructive fire.
Phylogenetic evidence for intratypic recombinant events in a novel human adenovirus C that causes severe acute respiratory infection in children
Authors: Wang Yanqun , Li Yamin , Lu Roujian , Zhao Yanjie , Xie Zhengde , Shen Jun , Tan Wenjie 
Published: March 2016

AbstractHuman adenoviruses (HAdVs) are prevalent in hospitalized children with severe acute respiratory infection (SARI). Here, we report a unique recombinant HAdV strain (CBJ113) isolated from a HAdV-positive child with SARI. The whole-genome sequence was determined using Sanger sequencing and high-throughput sequencing. A phylogenetic analysis of the complete genome indicated that the CBJ113 strain shares a common origin with HAdV-C2, HAdV-C6, HAdV-C1, HAdV-C5, and HAdV-C57 and formed a novel subclade on the same branch as other HAdV-C subtypes. BootScan and single nucleotide polymorphism analyses showed that the CBJ113 genome has an intra-subtype recombinant structure and comprises gene regions mainly originating from two circulating viral strains: HAdV-1 and HAdV-2. The parental penton base, pVI, and DBP genes of the recombinant strain clustered with the HAdV-1 prototype strain, and the E1B, hexon, fiber, and 100 K genes of the recombinant clustered within the HAdV-2 subtype, meanwhile the E4orf1 and DNA polymerase genes of the recombinant shared the greatest similarity with those of HAdV-5 and HAdV-6, respectively. All of these findings provide insight into our understanding of the dynamics of the complexity of the HAdV-C epidemic. More extensive studies should address the pathogenicity and clinical characteristics of the novel recombinant.
Hilfreiche Arme
Authors: Mau Jens 
Published: March 2016

In der Chirurgie ist die Robotik noch nicht weit gekommen. Roboter scheiterten am
klobigen Industriedesign oder am klinischen Workfl ow. Viele schaffen es vom Forschungsinstitut
gar nicht erst zum marktfähigen Produkt. Zurzeit gibt es auf dem Markt nur einen
echten Verkaufsschlager – doch der scheint die Branche jetzt wachzurütteln.
Knapper Faktor Mensch
Authors: Mau Jens 
Published: March 2016

Roboter können perfekt Autos zusammenschweißen. Aber sie können keine Kranken pflegen und heilen. Ärzte und Pflegekräfte werden unersetzlich bleiben, doch sie werden weniger. Antworten auf diese Herausforderung geben Experten auf dem Gesundheitskongress des Westens, der am 11. und 12. März 2009 in Essen stattfindet.
Sein Generalthema: Personal finden, Personal binden – der knappe Faktor Mensch.
Furchtlos in der Krise
Authors: Gaede Kirsten , Mau Jens , Zehnder Adalbert 
Published: March 2016

Während andere Branchen Umsatzeinbrüche im zweistelligen Prozentbereich verbuchen, blickt die Gesundheitswirtschaft gelassen in die Zukunft. Das hat vor allem damit zu tun, dass eine Diabetikerin zwar auf Sportwagen und Gucci-Tasche verzichten kann, nicht aber auf ihr Insulin. Das ist einfach formuliert, vielleicht zu einfach. Ein Blick in die
verschiedenen Branchen sorgt für Differenzierung.
Rebellion der Chefärzte
Authors: Pabst Inga 
Published: March 2016

Eklat im UKE: Drei Monate nach der Eröffnung des Klinikneubaus haben 15 Chefärzte beim Vorstand erheb­liche Mängel moniert. Offenbar ist das UKE dem um fast 17 Prozent gestiegenen Patientenzustrom nicht gewachsen.
Novel Highly Pathogenic Avian H5 Influenza A Viruses in Live Poultry Markets, Wuxi City, China, 2013−2014
Authors: Ma Mai-Juan , Chen Shan-Hui , Wang Guo-Lin , Zhao Teng , Qian Yan-Hua , Wu Meng-Na , Liu Ying , Gray Gregory C. , Lu Bing , Cao Wu-Chun 
Published: March 2016

Abstract
During 12 recent months of periodic influenza virus surveillance at 9 live poultry markets in Wuxi City China, we identified multiple highly pathogenic H5N6, H5N8, H5N2, and H5N1 avian influenza viruses. The variety of potentially pandemic viruses in this low-risk area is disconcerting and portends an increased pandemic threat.
Helping motivation and well-being of chronic pain couples: a daily diary study
Authors: Kindt Sara , Vansteenkiste Maarten , Loeys Tom , Goubert Liesbet 
Published: March 2016

Abstract
Receiving support from a romantic partner may yield benefits for individuals with chronic pain (ICPs), but may also carry unintended side effects. The conditions under which partner support provision yields (mal)adaptive effects deserve greater attention. Grounded in Self-determination theory, partners may provide help for autonomous or volitional (eg, enjoyment, full commitment) or rather controlled or pressured (eg, avoiding guilt and criticism) motives. This study examined associations between day-to-day fluctuations in partners' type of helping motivation and several outcomes, among partners and ICPs. Seventy couples, with 1 partner having chronic pain (75.7% female), completed a diary for 14 consecutive days. Daily helping motivation was assessed together with daily affect, relational conflict, and relationship-based need satisfaction. Partners (Mage = 55.14) additionally reported on daily helping exhaustion, whereas ICPs (Mage = 54.71) reported on daily pain intensity, disability, satisfaction with received help, and amount of received help. Providing autonomous help related to improvements in partners' affective (eg, positive affect), relational (eg, conflict), and help-specific (eg, exhaustion) functioning, which were accounted for by improvements in daily relationship-based psychological need satisfaction. Similarly, daily autonomously motivated help yielded a direct (ie, relational conflict; perceived amount of help) or indirect (ie, positive and negative affects; relational conflict; satisfaction with help, disability) contribution in explaining ICP outcomes—through improvements in ICPs' relationship-based psychological need satisfaction. Findings highlight the importance of a motivational and dynamic perspective on help provision within chronic pain couples. Considering reasons why a partner provides help is important to understand when partners and ICPs may benefit from daily support.
A randomized controlled trial of gabapentin for chronic low back pain with and without a radiating component
Authors: Atkinson J. Hampton , Slater Mark A. , Capparelli Edmund V. , Patel Shetal M. , Wolfson Tanya , Gamst Anthony , Abramson Ian S. , Wallace Mark S. , Funk Stephen D. , Rutledge Thomas R. , Wetherell Julie L. , Matthews Scott C. , Zisook Sidney , Garfin Steven R. 
Published: March 2016

Abstract
Gabapentin is prescribed for analgesia in chronic low back pain, yet there are no controlled trials supporting this practice. This randomized, 2-arm, 12-week, parallel group study compared gabapentin (forced titration up to 3600 mg daily) with inert placebo. The primary efficacy measure was change in pain intensity from baseline to the last week on treatment measured by the Descriptor Differential Scale; the secondary outcome was disability (Oswestry Disability Index). The intention-to-treat analysis comprised 108 randomized patients with chronic back pain (daily pain for ≥6 months) whose pain did (43%) or did not radiate into the lower extremity. Random effects regression models which did not impute missing scores were used to analyze outcome data. Pain intensity decreased significantly over time (P < 0.0001) with subjects on gabapentin or placebo, reporting reductions of about 30% from baseline, but did not differ significantly between groups (P = 0.423). The same results pertained for disability scores. In responder analyses of those who completed 12 weeks (N = 72), the proportion reporting at least 30% or 50% reduction in pain intensity, or at least “Minimal Improvement” on the Physician Clinical Global Impression of Change did not differ significantly between groups. There were no significant differences in analgesia between participants with radiating (n = 46) and nonradiating (n = 62) pain either within or between treatment arms. There was no significant correlation between gabapentin plasma concentration and pain intensity. Gabapentin appears to be ineffective for analgesia in chronic low back pain with or without a radiating component.
Agonist-dependence of functional properties for common nonsynonymous variants of human transient receptor potential vanilloid 1
Authors: Wang Sen , Joseph John , Diatchenko Luda , Ro Jin Y. , Chung Man-Kyo 
Published: March 2016

Abstract
Transient receptor potential vanilloid 1 (TRPV1) is a polymodal receptor activated by capsaicin, heat, and acid, which plays critical roles in thermosensation and pain. In addition, TRPV1 also contributes to multiple pathophysiological states in respiratory, cardiovascular, metabolic, and renal systems. These contributions are further supported by evidence that variations in the human TRPV1 (hTRPV1) gene are associated with various physiological and pathological phenotypes. However, it is not well understood how the variations in hTRPV1 affect channel functions. In this study, we examined functional consequences of amino acid variations of hTRPV1 induced by 5 nonsynonymous single-nucleotide polymorphisms (SNPs) that most commonly exist in the human population. Using electrophysiological assays in HEK293 cells, we examined 9 parameters: activation, Ca2+ permeation, and desensitization after activation by capsaicin, acid, and heat. Our results demonstrated that the 5 SNPs differentially affected functional properties of hTRPV1 in an agonist-dependent manner. Based upon the directionality of change of each phenotype and cumulative changes in each SNP, we classified the 5 SNPs into 3 presumptive functional categories: gain of function (hTRPV1 Q85R, P91S, and T469I), loss of function (I585V), and mixed (M315I). These results reveal a spectrum of functional variation among common hTRPV1 polymorphisms in humans and may aid mechanistic interpretation of phenotypes associated with nonsynonymous hTRPV1 SNPs under pathophysiological conditions.
DIRC3and close toNABP1Genetics Polymorphisms correlated with Prognostic Survival in Patients with Laryngeal Squamous Cell Carcinoma
Authors: Shen Zhen , Ren Wanli , Bai Yanxia , Chen Zhengshuai , Li Jingjie , Li Bin , Jin Tianbo , Cao Peilong , Yuan Shao 
Published: March 2016

AbstractLaryngeal squamous cell carcinoma (LSCC) is one of the most common and aggressive malignancies in the upper digestive tract that has a high mortality rate and a poor prognosis. Prognostic factors were determined through multivariate Cox regression analysis. The overall survival rates were calculated by the Kaplan-Meier method. The SPSS statistical software package version 17.0 (SPSS Inc., Chicago, IL, USA) was used for all analyses. Median follow-up was 38 (range 3-122) months and the median survival time was 48 months. We adjusted to confounding factors (total laryngectomy, poor differentiation, T3-T4 stage, N1-N2 stage, III-IV TNM stage) into multivariate Cox proportional hazards model, we confirmed rs11903757 GT genotype (HR = 2.036; 95% CI, 1.071 - 3.872; p = 0.030) and rs966423 TT genotype (HR = 11.677; 95% CI, 3.901 - 34.950; p = 0.000) were significantly correlated with prognostic survival of patients with LSCC compared with rs11903757 TT genotype and rs966423 CC genotype, respectively. Our research provided new evidence for patients with LSCC, it seemed to be the first that demonstrated rs11903757 GT genotype on chromosome 2q32.3 close toNABP1and rs966423 TT genotype in the intron region ofDIRC3on chromosome 2q35 predict poor prognostic survival in patients with LSCC.
Critical influence of relational governance on relationship value in strategic supply management
Authors: Yeh Ying-Pin 
Published: March 2016

Purpose
This paper aims to explore the enhancement of value-added characteristics in strategic supply management by considering manufacturers’ willingness, opinions on relational governance and the effect of relational governance on relationship value.

Design/methodology/approach
After a literature review, this study identified the antecedents of relational governance affecting manufacturers, and explored the effect of relational governance on relationship value, taking relationship quality and interfirm learning as the intervening constructs. Data collected from 259 valid questionnaires completed by purchasing managers for the top 2,000 Taiwanese manufacturers were assessed using a structural equation model.

Findings
The results indicated that relational governance is directly and positively correlated with relationship quality and interfirm learning; relational quality and interfirm learning are directly and positively correlated with relationship value; and consumer orientation and management innovation are directly and positively correlated with relational governance.

Research limitations/implications
The high explanatory power of the results of the deduced model in this research helps to explain the relational governance of manufacturers toward the suppliers. However, the factors affecting the sustainability of cooperative relationships in service contexts might differ.

Practical implications
Relational governance complements the adaptive limits of contracts by fostering the continuance of exchange and entrusting both parties with mutually agreeable outcomes. Relational governance affects manufacturers’ ability to flexibly adapt and overcome uncertainty in the supply chain relationship.

Originality/value
This study investigated the relationship among governance features that support interorganizational relations and developed precise measures of relational governance. The effect of relational governance on the evaluation of relationship value was examined.
Is innovation research contingent on competitive context?
Authors: Pehrsson Tobias 
Published: March 2016

Purpose
The purpose of this paper is to extend the understanding of innovation research and its contextual boundaries.

Design/methodology/approach
The paper opts for a systematic review of literature on innovation. Based on research in the agricultural and forest industries, it analyzes differences between research conducted in a competitive context of strong rivalry and research in a context characterized by strong buyer power. In particular, the review compares types of innovation under investigation and the level at which the innovation occurs.

Findings
It was found that competitive context significantly separates the type of innovation under investigation and innovation at different levels. Thus, the findings provide insights on the importance of competitive context to innovation research.

Research limitations/implications
The findings have implications for the understanding of the sources and directions of innovation, and the formation of innovation at the firm and industry levels. The review also provides a relevant foundation for further research.

Practical implications
The review provides a ground for managerial decision-making regarding innovation. A manager wishing to innovate is advised to evaluate the competitive context. The evaluation is crucial, as the context facilitates different types and levels of innovation.

Originality/value
The review is unique in its emphasis that reviewing studies of innovation requires the consideration of competitive context.