bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue Factor Pathway Inhibitor 2 (TFPI2) is a Serum Biomarker for Clear Cell Renal Carcinoma
Authors: Ito Hiroki , Jikuya Ryosuke , Myoba Shohei , Tatenuma Tomoyuki , Noguchi Go , Ueno Daiki , Ito Yusuke , Komeya Mitsuru , Muraoka Kentaro , Yao Masahiro , Hasumi Hisashi , Nakaigawa Noboru , Makiyama Kazuhide 
Published: October 2024

Abstract

Tissue factor pathway inhibitor 2 (TFPI2), a serine protease inhibitor, has emerged as a promising serum biomarker for ovarian clear cell carcinoma. We evaluated the efficacy of TFPI2 as a biomarker for renal cell carcinoma (RCC). This single-center study enrolled patients with RCC who underwent radical nephrectomy or tumor biopsy, and healthy volunteers. Preoperative serum samples were collected from patients, and some patients underwent postoperative sampling. Serum TFPI2 levels were measured using automated enzyme-linked immunosorbent assay. Expression of TFPI2 in each cell type was evaluated using single-cell RNA sequencing. Survival analyses according to TFPI2 expression levels were performed based on publicly available databases. Serum TFPI2 was significantly elevated in patients with RCC compared to healthy volunteers, particularly those with clear cell histology. Metastatic RCC tumors exhibited higher TFPI2 levels than localized RCCs. Moreover, higher TFPI2 levels correlated with higher Fuhrman grades in clear cell RCC. Analyses of publicly available databases further supported this finding, showing an association between TFPI2 expression and overall survival, particularly in clear cell RCC. Single-cell RNA sequencing confirmed TFPI2 expression in clear cell RCC and normal kidney tubular epithelial cells, supporting its relevance. TFPI2 has emerged as a potential serum biomarker for RCC, offering avenues for improved detection and prognostication, similar to its utility in ovarian clear cell carcinoma. However, its clinical utility warrants further exploration in routine diagnostic and monitoring practices for patients with RCC.
Nosocomial Infection in Patients Treated with Venoarterial Extracorporeal Membrane Oxygenation: Incidence, Risk factors, and Outcomes
Authors: Zhou Hao , Ding Tao , Shi Yutong , Zhu Yi , Chen Chen , Zhang Zhongman , Mei Yong , Lv Jinru , Li Wei , Chen Xufeng 
Published: October 2024

Abstract

Background
The success rate of venoarterial extracorporeal membrane oxygenation (VA-ECMO) is related to complications such as nosocomial infection (NI), with multidrug-resistant organisms (MDROs) infection posing the toughest challenge, however, the incidence, risk factors, and prognosis of NI during VA-ECMO are unclear.
Methods
We performed a single-center, retrospective analysis of 152 patients receiving VA-ECMO. Patients were categorized into NI and non-NI groups, depending on whether pathogenic microorganisms were isolated between 48h after VA-ECMO initiation and within 24h weaning from VA-ECMO. Multivariate logistic regression analysis was performed to identify the independent risk factors of NI.
Results
The incidence of NI during VA-ECMO was 38.82% (59/152), and 71 microbial strains were isolated from the cultures, with Gram-negative bacteria accounting for 73.24% (52/71) and MDROs for 63.38% (45/71). The chi-square test showed that the probability of percutaneous dilatational tracheotomy (PDT) was elevated in the NI group (25.42% vs 8.6%). The rank sum test found that the NI group had longer hospitalization (18 vs 12 days), intensive care unit (ICU) stay (16 vs 9 days), and duration of VA-ECMO (6.63 vs 5.01 days), and invasive mechanical ventilation (IMV, 11 vs 6 days). Multivariate logistic regression analysis revealed that the occurrence of NI was independently associated with ICU stay (OR 1.128; 95%CI 1.004 ~ 1.268; P = 0.043) and the need for PDT (OR 3.459; 95%CI 1.051 ~ 11.389; P = 0.041).
Conclusions
The Gram-negative bacilli was the most common pathogens for NI during ECMO, with MDROs being the predominant species. The occurrence of NI in patients caused a prolonged ICU stay and increased probability of PDT.
Trial registration
ChiCTR1900026105 (Registration Date 20190921).
Molecular epidemiological analysis of Influenza viruses in Influenza-like illness cases: a retrospective study in Chongqing Hi-Tech Zone, China (2021-2024)
Authors: Cai Wei , Deng Yuhang , Xu Pan , Xue Jianjiang 
Published: October 2024

Abstract

The seasonal epidemiology and high variability of influenza viruses result in yearly differentiation of main pandemic strains. Effective prevention and control of influenza epidemics require enhanced surveillance, timely updates of vaccine components.Therefore, it is crucial to establish a continuous surveillance system to track epidemiological trends and develop appropriate vaccination and preventive measures.A retrospective analysis was conducted on the molecular epidemiological characteristics of influenza viruses in influenza-like cases at a hospital in Chongqing Hi-Tech Zone from 2021 to 2024. Among 39,986 ILI specimens tested using the immunocolloid gold method, 6,616 influenza viruses were detected, with a detection rate of 16.54%. Infections included 4,464 influenza A viruses (67.50%), 2,033 influenza B viruses (30.73%), and 119 co-infections of influenza A and B viruses (1.77%). From November 2021 to September 2022, the peak detection of influenza A virus occurred in January 2022, and influenza B virus peaked in April 2022. From July 2022 to March 2023, the influenza A virus peaked in December 2022, while the influenza B virus peaked in January 2023. Influenza B virus detection lagged behind influenza A virus detection. In this region, H3N2 was the predominant subtype of influenza A, and Victoria was the main subtype of influenza B. The "H1" subtype of influenza A did not appear throughout 2023 but accounted for 82% in January 2024 and 16% in February 2024. The "Yamagata" subtype of influenza B did not appear from 2023 to 2024. Influenza epidemics in the winter and spring seasons in Chongqing Hi-Tech Zone were predominantly caused by influenza A, with influenza B also circulating. Influenza A strains were mainly H3N2, while influenza B strains were primarily Victoria. This study provides essential data and a scientific basis for developing and optimizing influenza prevention and control strategies.
Growth Associated Protein 43 (GAP-43) predicts brain amyloidosis in Alzheimer’s Dementia Continuum: an [18F] AV45 study
Authors: Nemati Rezvan , Sadeghi Mohammad , Saberian Parsa , Ashlaghi Ahmadreza Sohrabi , Mardani Sajjad , Abadi Sina Jafari Hossein , Yaghoobpoor Ali , Heydari Atefeh , Khoshroo Niloofar , Rahnama Yassin , Mayeli Mahsa 
Published: October 2024

Abstract

Background
Alzheimer's disease (AD) is a global health concern with a rising prevalence. Growth Associated Protein 43 (GAP-43) is a crucial protein for neuronal growth and synaptic plasticity, essential for maintaining healthy brain function. In AD, changes in GAP-43 levels have been observed, potentially indicating synaptic dysfunction and neurodegeneration. This study investigates the potential of GAP-43 as a biomarker in AD by analyzing its correlation with amyloid-beta (Aβ) pathology, a hallmark feature of the disease using [18F] AV45.
Methods
We examined 1639 participants using a dataset extracted from the Alzheimer's Disease Neuroimaging Initiative (ADNI).
Results
A total of 226 subjects meeting the eligibility criteria were recruited from the ADNI dataset for enrollment. These individuals were categorized into three groups: 77 cognitively normal (CN) individuals, 111 with mild cognitive impairment (MCI), and 38 AD. Our results reveal elevated CSF GAP-43 levels in AD, and GAP-43 exhibited a stronger association with tau pathology than with Aβ. The study establishes a robust positive correlation between GAP-43 and [18F] florbetapir PET ([18F] AV45), a marker for Aβ plaques, independent of cognitive status. Additionally, logistic regression identified GAP-43) as significant predictors of AD.
Conclusion
The diagnostic accuracy of [18F] AV45, combined with GAP-43, enhances understanding of AD pathology. This study sets the stage for future research on GAP-43's trajectory in disease progression and the molecular mechanisms linking GAP-43 and amyloid-beta. The findings suggest promising avenues for novel therapeutic targets, contributing to advancements in early detection and treatment strategies for AD.
Initial Clinical Experiences of Robotic Distal Gastrectomy for Gastric Cancer Using the Da Vinci TM SP System
Authors: Ito Ayaka , Nakauchi Masaya , Fujita Masahiro , Umeki Yusuke , Suzuki Kazumitsu , Serizawa Akiko , Akimoto Shingo , Watanabe Yusuke , Tanaka Tsuyoshi , Shibasaki Susumu , Inaba Kazuki , Uyama Ichiro , Suda Koichi 
Published: October 2024

Abstract

Purpose
Reduced-port surgery has been utilized in gastric cancer surgery but was not predominantly used due to its high technical difficulty. A new single-port surgical robot named da Vinci™ SP System (DVSP) was launched and eventually approved for clinical use in Japan in November 2022. We initiated robotic gastrectomy for gastric cancer using DVSP in March 2023. Here, we report our initial experiences and assessments of the feasibility and safety of robotic gastrectomy for gastric cancer using DVSP.
Methods
This single-center retrospective study included 20 patients with gastric cancer who underwent robotic gastrectomy with DVSP from March 2023 to April 2024. The primary endpoint was the postoperative complication rate within 30 days postoperatively. Secondary endpoints were surgical outcomes, including intraoperative adverse events, operative time, blood loss, and the number of dissected nodes.
Results
Of the 20 patients, 6 (30.0%) were male. The median age was 76.5 years. Tumors in the middle to lower stomach were observed in 20 patients (100.0%), including 18 (90.0%) and 2 (10.0%) with clinical stages I and II diseases, respectively. All patients underwent distal gastrectomy. The postoperative complications of Clavien–Dindo grade ≥ II occurred in 3 (15%) patients. Intraoperative adverse events, including conversion to other approaches, were not observed. All patients underwent R0 resection. The median operative and console times were 283.5 and 240 min, respectively. The median blood loss was 11.5 mL with 49 dissected nodes.
Conclusion
This study revealed the safe performance of robotic distal gastrectomy with standard lymphadenectomy for gastric cancer using DVSP.
The construction and validation of integrated immune and metabolic gene signatures for clinical prognostic model of lung squamous cell carcinoma
Authors: Xue Haoyuan , Li Hongwei , Han Songyan , Zhang Xiaqin , Bu Peng , Liang Hua 
Published: October 2024

Abstract

Background:
Lung squamous cell carcinoma (LUSC) has a poor prognosis due to the lack of effective targeted therapies, and its incidence has dramatically increased in recent years. Therefore, new prognostic markers are urgently needed. Since tumour immune and metabolic heterogeneity can influence LUSC prognosis, systematic combinatorial analysis of immune-related and metabolism-related genomic patterns may identify such markers. Thus, this study aimed to construct a novel predictive model based on immune-related and metabolism-related genes for prognostic stratification in LUSC.
Methods:
Transcriptomic as well as clinical data of 502 and 43 LUSC cases were downloaded from The Cancer Genome Atlas Program (TCGA) and the Gene Expression Omnibus (GEO) databases. Core LUSC subtype genes were identified using nonnegative matrix factorization (NMF). A risk model based on prognostic LUSC genes was constructed using machine learning, LASSO regression, and multivariate Cox regression. Subsequently, we defined low-risk and high-risk expression profiles comprising these markers and revealed survival differences. Gene-Set Enrichment Analysis of these marker genes revealed the active pathways in the high-risk group versus the low-risk group. Diverse clinical treatment strategies for both risk groups were also examined. Immunohistochemical validation involving 42 patients demonstrated the expression patterns of the identified genetic markers.
Results:
The constructed risk model for nine LUSC genes effectively stratified patients into low-risk and high-risk subgroups with different survival rates, tumour mutation burden, and response to clinical therapy. High expression levels of NRTN, CYP2C18, TSLP, MIOX, and RORB and low expression levels of HBEGF, SERPIND1, PTGIS, and LBP were correlated with high survival rates. The high-risk group was strongly associated with immune pathways, and the low-risk group was strongly associated with metabolism pathways. The expression of model markers was stronger in tumours than in adjacent normal tissues.
Conclusions:
Six immune-related and three metabolism-related genes were identified as prognostic markers of LUSC, with their expression levels significantly associated with the survival rate. The prognostic model constructed using these markers has a strong predictive power. Accordingly, the findings are expected to guide decisions on treatment strategies.
Measuring and characterizing the quality of child care in Brazilian primary health care: a latent class analysis
Authors: Flores-Quispe Maria del Pilar , Passos Michelle , Almeida Josemir R. , Santos Ythalo H. S. , Aquino Rosana , Vieira-Meyer Anya PGF , Luz Leandro Alves , Anjos Eduarda Ferreira , Lima Acácia M. P. , Martufi Valentina , Ortelan Naiá , Ichihara Maria Yury Travassos , Barreto Mauricio L. , Amorim Leila D.A.F. , Pinto-Junior Elzo Pereira 
Published: October 2024

Abstract

Background
Ensuring the highest quality of care for children in Primary Health Care is essential to prevent or control risks that compromise a child's growth and development. This study aimed to characterize the quality of child health care provided by Primary Health Care (PHC) teams in Brazil and explore its relationship with municipal characteristics.
Methods
Using data from the external assessment of the first cycle of the National Program for Access and Quality Improvement in Primary Care, this cross-sectional study evaluated 16,566 PHC teams from the Family Health Strategy. Nine binary indicators of the quality of child care were created based on the recommendations of the Brazilian Ministry of Health. We used latent class analysis to characterize the quality care. Through a multinomial logistic regression, we assessed the association between belonging to a given latent class of quality of care and the municipal covariates region and Brazilian Deprivation Index.
Results
Three patterns (latent classes) of quality of care were identified: high, intermediate and low adequacy. The pattern labelled "High adequacy” included 31.2% of the PHC teams and had higher conditional probabilities in all nine indicators (over 70%). The pattern “Intermediate adequacy” involved 53.3% of PHC teams, and the "Low adequacy” pattern included only 15.4% of teams. The chance for a PHC team to belong to the "High adequacy” pattern was over two times (OR = 2.34; 95%CI: 1.15–4.76) in the Northeast region compared to the Center-West region. Relating to the Brazilian Deprivation Index, for PHC teams located in municipalities with moderate and low deprivation, the chance of belonging to the "High adequacy” pattern was 2.04 (95%CI: 1.44–2.89) and 9.08 (95%CI: 4.54–18.14) times, respectively, compared to the high deprivation municipalities.
Conclusions
This study identified three patterns of quality of child care. Most of the PHC teams were included in the “Intermediate adequacy” pattern. Furthermore, the quality of care was associated with the municipality characteristics. Our findings demonstrated that the methodology used in this study allowed us to characterize the quality of care in a more consistently way than with a conventional method using a set of indicators constructed from a national dataset.
Increasing the Number of Biopsy Cores Around Prostate Cancer Targeted Lesions in the Transition Zone to Improve Biopsy Accuracy: A Combined Retrospective and Prospective Study
Authors: Chen Xin , Huang Chen , Zhou Chenchao , Li Yu , Huang Renpeng , Bao Jie , Lin Yuxin , Truß Michael C. , Hou Jianquan , Huang Yuhua , Wei Xuedong 
Published: October 2024

Abstract

Background
Prostate biopsy (PBx) plays a pivotal role in diagnosing prostate cancer (PCa). However, the prostate biopsy results for transition zone (TZ) tumors were found to be less accurate than those for peripheral zone tumors. The objective of this study was to identify potential under-detection of TZ PCa and to validate a new biopsy template that increases the number of cores around TZ targeted lesions to improve biopsy accuracy for TZ tumors.
Materials and Methods
This study comprised two components: a retrospective analysis and a randomized clinical trial. The retrospective study included 217 patients who underwent radical prostatectomy following "12 + X" template transperineal transrectal ultrasound-magnetic resonance imaging-fusion PBx between 2018 and 2021 at our institution. The clinical trial investigated biopsy efficacy for clinically significant PCa (csPCa) in 400 patients who underwent either modified "18 + X" template PBx for TZ lesion or "12 + X" template PBx following systematic sampling between 2022 and 2023 at our center.
Results
The retrospective analysis revealed that the "12 + X" template, comprising 4 cores in TZ, failed to adequately detect csPCa in the TZ. “18 + X” template was constructed based on the results of retrospective analysis. Conversely, in the prospective trial, the "18 + X" template, with 10 cores in TZ, outperformed the "12 + X" template in detecting TZ csPCa without a corresponding increase in complications. Building upon the result, "18 + X" template was improved to the "New 12" and "New 12 + X" templates, with 8 cores in TZ and 4 cores in peripheral zone, while maintaining the same csPCa detection rate for TZ patients.
Conclusions
Our study demonstrated that increasing the number of biopsy cores around targeted lesions in the TZ improves biopsy accuracy and reduces the number of biopsies in non-targeted regions without compromising overall accuracy. The new template offers a promising approach to improve biopsy for patients with TZ lesions.
Trial Registration:
This study was approved by Clinical trial registration in China Clinical Trial Registry (ChiCTR2200056386). Registered 04 February 2022, https://www.chictr.org.cn
Stent placement can achieve same prognosis as endoscopic nasobiliary drainage in treatment of bile leakage after liver transplantation
Authors: Gu Yangjun , Chen Zhitao , Li Qiyong 
Published: October 2024

Abstract

Background
Both stent placement and endoscopic nasobiliary drainage are commonly employed for treating bile leakage following liver transplantation. However, little research compared the advantages of both.
Methods
Data of patients with bile leakage after liver transplantation were retrospectively collected in our center. The efficiency and prognosis were compared between two groups.
Results
We retrospectively collected data from total 50 patients diagnosed with bile leakage within one month after liver transplantation. Among them, 40 patients underwent endoscopic nasobiliary drainage (ENBD) and 9 patients received stent placement. Baseline patient characteristics, except white blood cell count (WBC), total bilirubin (TB), and direct bilirubin (DB) levels, were comparable between the two groups. Additionally, there was no significant difference in the rates of reconveyance or post-operative biliary stricture.
Conclusion
Both stent placement and endoscopic nasobiliary drainage are recommended for treating bile leakage after liver transplantation, as they exhibit similar efficacy and prognosis.
NMO-IgG causes primary retinal damage without optic nerve injury
Authors: Chen Biyue , Zhou Huanfen , Yang Kaishu , Song Honglu , Xu Quangang , Sun Mingming , Xu Xintong , Li Yuyu , Li Qianqian , Yu Yanyan , Wei Shihui , Chen Tingjun 
Published: October 2024

Abstract

Background
Neuromyelitis Optica (NMO) is a neuroimmune disorder primarily driven by autoantibodies against aquaporin 4 (AQP4), known as NMO-IgG. Although the mechanisms underlying NMO-IgG-induced retinopathy are not fully understood, the high expression of AQP4 in retinal Müller cells suggests a direct interaction that may trigger inflammatory processes in the retina. Previous studies indicate that microglia play a critical role in mediating immune responses, leading to neuronal dysfunction.
Methods
NMO-IgG obtained from clinical patients was administered via intravitreal injection to female C57BL/6 mice. Techniques such as optical coherence tomography (OCT), electroretinography (ERG), real-time fluorescence quantitative PCR (RT-qPCR), and immunofluorescence analyses were used to assess retinal changes. The potential for reversing retinopathy was explored by depleting microglial cells using the CSF1 receptor inhibitor PLX3397. Additionally, a Transwell co-culture system of MIO-M1 (Müller cells) and BV2 (microglia) cells was established to study their interactions.
Results
Intravitreal injection of purified NMO-IgG in mouse models led to its deposition in the retina and downregulation of AQP4 in Müller cells. Vascular leakage was observed, alongside retinal dysfunction characterized by thinning of the retinal nerve fiber layer (RNFL) and loss of retinal ganglion cells (RGCs), consistent with ERG findings. By day 7, C3 expression was upregulated in Müller cells, followed by microglial activation. Significant morphological changes in microglia were noted, with increased expression of iNOS and C1q, indicating substantial activation. Ablating microglia significantly mitigated NMO-IgG-induced injury to RGCs. In vitro, NMO-IgG-treated MIO-M1 cells secreted higher levels of C3, enhancing the activation and migration of BV2 cells compared to controls.
Conclusions
The retinal dysfunction observed in NMO may primarily be linked to the activation of Müller cells by NMO-IgG, leading to increased C3 secretion, which in turn activates microglia. Therapeutic strategies targeting Müller cell-microglia interactions in NMO-IgG-induced retinopathy could be promising in addressing the underlying retinal pathology in this condition.