bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Roseosiphovirus Infecting Dinoroseobacter shibae DFL12T Represents a New Genus
Authors: Wei Nana , Lu Longfei , Ding Bo , Cai Lanlan , Yang Yunlan 
Published: October 2024

Abstract

Bacteria belonging to the Roseobacter clade are key players in marine ecosystems, contributing significantly to carbon and sulfur cycles. Marine viruses, particularly those targeting Roseobacter, play crucial roles in regulating microbial communities and biogeochemical processes. Despite their importance, phages infecting the Roseobacter clade remain poorly understood. In this study, a novel roseophage, vB_DshS-R26L (R26L), infecting Dinoroseobacter shibae DFL12T, was isolated and characterized in terms of physiological and genomic properties. R26L is a siphovirus with an elongated head and a long, non-flexible tail. The phage has a narrow host range and demonstrates a long infection cycle with a latent period of 3.5 hours and a burst size of 22 plaque-forming units (PFU cell− 1). R26L possesses a circular, double-stranded DNA genome of 79,534 bp with a G + C content of 62.59%, encoding a total of 116 open reading frames. Notably, seven auxiliary metabolic genes, including those related to phosphate metabolism and queuosine biosynthesis, were identified. Phylogenetic and taxonomic analyses revealed that R26L represents a new genus, with its highest intergenomic similarities being 54.7% to another roseophage, R5C, and 42.0% to Pan1. By elucidating the unique characteristics of R26L, this study highlights the complexity of phage infections and the genomic diversity of roseophages, offering valuable insights into the ecological significance of Roseobacter–phage interactions in marine environments.
Extracellular vesicles modulate growth and stress adaptation in Fusarium circinatum
Authors: Ratsoma Francinah , Mokoena Nthabiseng , Mpupa Sokunene , Santana Quentin , Wingfield Brenda , Steenkamp Emma , Motaung Thabiso 
Published: October 2024

Abstract

The molecular mechanisms influencing Fusarium circinatum, an important pine tree pathogen, remain partially understood. We recently reported a biofilm-mediated response in this fungus, which supports its adaptation to harsh conditions including heat stress. Herein, we report that biofilm extracellular vesicles (EVs) play a key role in this adaptive response. The EVs were purified from planktonic and biofilm cells via differential ultracentrifugation and size exclusion chromatography. Their subsequent application to fungal cells revealed the capacity of biofilm-derived EVs (bEVs) to promote conidial viability and germination. When bEV-treated conidia were cultured in the presence of carbon sources (PM1), nitrogen sources (PM3B), and chemical sensitivity agents (PM21D), a delayed nutrient utilization and adaptation to antimicrobial agents such as nystatin, was observed. Furthermore, exogenous application of bEVs on mono- and polymicrobial biofilms significantly enhanced biomass and matrix production, with EVs derived from heat-stressed biofilm (45 ºC, 1 hour) showing more effectiveness at promoting biomass production and resistance to the antifungal agent, tebuconazole. This is consistent with the biofilm’s heat resistance previously reported for F. circinatumbiofilms. Taken together, our work provides novel insights into the EV-mediated molecular interactions that modulate environmental responses in F. circinatum.
Políticas educativas interculturales en México de 1980 a 2022
Authors: Castro Porcayo David 
Published: October 2024

El artículo tiene el propósito de explorar cómo se ha abordado la dimensión de la interculturalidad en las políticas educativas, en un periodo que abarca de los años anteriores a 1980 a 2022. El propósito fue entender cómo discursivamente se han objetivado políticas educativas en relación con la interculturalidad. La investigación tiene un enfoque cualitativo, un método bibliográfico y de análisis de discurso. Se identificaron tesis que han funcionado como “puntos de capitón”, término empleado por Lacan (2009) para explicar cómo el lenguaje, que está en constante desplazamiento, necesita estos puntos para estabilizar momentáneamente el sentido del discurso. Se ubicaron tres periodos aproximados que abarcan de antes de 1980, de 1982 a 2018 y 2018 a 2022, y se caracterizan por sus circunstancias históricas que determinaron los fines de la educación intercultural.
Application of SWAT to assess peatland forestry impacts on water quality
Authors: Bhattacharjee Joy , Marttila Hannu , Molina Navarro Eugenio , Kløve Bjørn 
Published: October 2024

ABSTRACT

Peatland drainage can affect the natural state of hydrological conditions and nutrient loading but is rarely included in catchment-scale models. To understand the gap, we aimed to use the soil and water assessment tool (SWAT) to observe drained peatlands and their properties to predict nutrients and suspended solids (SS) in the peatland-dominated Simojoki catchment. We integrated drainage networks in SWAT to (i) identify drainage parameters; (ii) determine annual loads and mean concentrations of SS, organic phosphorus (Org-P), total phosphorus (TP), organic nitrogen (Org-N), and total nitrogen (TN); (iii) understand spatial variation of nutrients and SS; and (iv) investigate uncertainty ranges for the estimates. The calibrated model showed a 9.6 per cent bias between the simulated flow and the observations with low-to-medium loading variations for the water quality parameters. For Org-N and TN, the highest loading per year was at the downstream outlet, whereas for SS, Org-P, and TP, it was higher at the upstream outlet of the catchment. This approach of representing the drained peatland in SWAT indicated maximum spatial distributed load in the peat soil in the clear-cut area and can be beneficial in future hydrological modelling efforts in identifying the status of nutrients or SS.
Urban Water System of the National Capital Territory (NCT) of Delhi: A Comprehensive Study
Authors: Parveen Adeeba , Ahmad Syed Naushad 
Published: October 2024

ABSTRACT

This study examines the intricate relationship between Delhi's urban water system and environmental factors, with a specific emphasis on the challenges posed by urbanization. It investigates the present conditions of the water resources, infrastructure, and governance in the National Capital Territory (NCT) of Delhi and identifies significant difficulties, such as depletion of groundwater and inadequate access to water. Additionally, it explores innovative approaches to strengthen supply internalization, such as rainfall harvesting, wastewater recycling, and demand management strategies. Therefore, this document establishes a path for future research endeavors and policy suggestions that aim to promote a fair, effective, and environmentally sustainable future for water in Delhi.
Solar-enhanced DCMD: Dual-tank system for superior efficiency and viability
Authors: Alsehli Mishal 
Published: October 2024

ABSTRACT

This study investigates a novel solar-powered direct contact membrane distillation (DCMD) system designed to significantly enhance desalination efficiency and economic feasibility. The system features a dual-tank configuration integrated with a solar collector, which optimizes thermal energy storage and ensures consistent feedwater temperatures. Therefore, continuous operation is possible despite fluctuations in solar radiation. An assessment of the system's performance under various solar radiation conditions predicted an average daily permeate flux of 14.4 kg/m2 and a specific energy consumption of 1810 kJ/kg, indicating superior thermal efficiency. The economic analysis revealed a competitive levelized water cost of $25.75 per cubic meter, highlighting the system's potential as a viable alternative to conventional desalination technologies. These findings suggest that the proposed DCMD system is a leading technology in the pursuit of sustainable desalination. Considering its thermal management and cost-effectiveness, the system presents a transformative solution for sustainable freshwater production in regions with abundant solar resources.
Navigating the definition of urban flooding: A conceptual and systematic review of the literature
Authors: Alves Priscila B. R. , Amanguah Ebenezer , McNally Devin , Espinoza Maria , Ghaedi Hamed , Reilly Allison C. , Hendricks Marccus D. 
Published: October 2024

ABSTRACT

Urban flooding is a pervasive global risk, posing a great challenge to urban planners, policymakers, and particularly communities. This paper reviews the literature to analyze how urban flooding is defined across scientific disciplines. Our objectives are to uncover the elements used to define urban flooding and evaluate how these elements can impact future research and practice. A key difficulty is the lack of a consistent, comprehensive definition that captures both physical and social dimensions of urban flooding. Current definitions often focus solely on physical aspects (e.g., rainfall, infrastructure) or social impacts, rarely integrating both. This fragmentation hinders effective flood risk management and interdisciplinary collaboration. Our contribution is a multifaceted definition incorporating spatial and social concerns, including water origins, built environment characteristics, and local community aspects. We introduce the ‘Urban Water Transect’ concept to illustrate the continuum of flood risk across urban zones, addressing a gap in the literature. The analysis reveals that many papers discuss flooding causes without providing an explicit definition. Urban flooding is predominantly defined based on water source, imperviousness, and drainage infrastructure. Future research should adopt an interdisciplinary perspective considering both physical and social aspects, potentially transforming urban flood risk management.
Characteristics of liquid sugar from old oil palm trunk sap as affected by processing methods
Authors: Nurdjanah Siti , Hasanudin Udin , Setiyoko Febri , Yuliandari Puspita , Nawansih Otik , Utomo Tanto Pratondo 
Published: October 2024

The sap of old oil palm trunks contains appreciable sugar, but it has not been considered a potential sugar source. This study aimed to find the best method for producing oil palm liquid sugar that complies with the Indonesian National Standard (SNI) The non-factorial experiment was arranged in a Completely Randomized Block Design (CRBD) with 6 replications. The treatments were a combination of lime-rotary vacuum evaporation (P1), without lime-vacuum rotary evaporation (P2), lime-atmospheric evaporation (P3); without lime-atmospheric evaporation (P4). The parameters observed were pH, °brix, reducing sugar, color, aroma, and overall acceptance. The data were subjected to ANOVA to determine the effects of the treatments and then, continued testing using HSD at α 0,05 for means separation. The best treatment was found on liquid sugar processed using lime, and atmospheric evaporation with brix of 68.75%, pH of 5.4, moisture content of 17.74, ash content of 0.86%, reducing sugar of 44.31%, sensory score of color, aroma, and overall acceptance were f 3.42 (brownies yellow), 4.4 (like), and 4.04 (like).Keywords: lime, liquid sugar, old oil palm trunk, sensory, vacuum rotary evaporator
The Impact of Job Stress on Job Satisfaction among Nurses during Covid-19 Pandemic: The Moderating Role of Psychological Capital
Authors: Alkhraishi Mohammed Yousef , Almashayekh Maysoon Fayiz , Yesiltas Mehmet 
Published: October 2024

The COVID-19 disease is a calamity that may leave a dreadful psychological indentation on nurses. Nurses take all the necessary measures to protect themselves physically and psychologically.
This global pandemic demands a firmly secured nursing staff engagement in clinical management in pursuance of public safety. In the interest of sustaining psychological and physical well-being, nurses should be entitled to fair compensation, access to counseling for mental health care support, and provide job satisfaction, empowering these professionals to survive this crisis and beyond.
This study is proposed to provide baseline information on the impact of job stress on Al Dhafra hospitals nurses' job satisfaction during the covid-19 pandemic and the effect of psychological capital as a moderator. Furthermore, the study will open a new aspect for further research to improve and reduce its adverse effects. In addition, it can be used as a reference to create, improve, or modify guidelines and existing protocols, and it can also serve as a reasonable contribution study for further nursing management in future crises.
The study employed a quantitative correlation research design, and an online survey was conducted and participated in by 255 nurses who are in direct contact with COVID-19 patients in Al Dhafra hospitals in UAE.
The present study's findings indicate a negative relationship between job stress and job satisfaction; on the other hand, The findings also revealed a positive moderating effect of psychological capital on the relationship between job stress and satisfaction.
The current study has important implications for hospitals in developing managerial, training, and strategic policies. According to the findings of this study, job stress has a significant impact on nursing satisfaction. In light of this study, hospitals can improve their job satisfaction. Furthermore, this study can be applied to other cultural and demographic contexts to reduce employee stress.
Changes in ruminal fermentation and rumen bacteria population in feedlot cattle during a high lipid diet adaptation
Authors: Vesga Daniela A. , Granja-Salcedo Yury T. , Costa Rayanne V. , Gomes Kenia L. , Alves Carvalho , Narvaez Hector J. , Berchielli Telma T. 
Published: October 2024

Abstract
This study aimed to investigate changes in feed intake and ruminal environmental parameters during a high-lipid diet transition in cattle. Eight Nellore steers were fed a control diet composed of 30% hay and 70% concentrate for 21 days, followed by the inclusion of 60 g/kg dry matter of soybean oil for 21 days. The DM intake expressed as a percentage of BW 21 days after lipid inclusion was lower (1.75% BW) than that observed during the control diet feeding (1.81% BW) (P<0.01). Steers fed the control diet had a lower pH than the ruminal pH recorded on days 7, 14, and 21 after lipid inclusion (P=0.034). Lower total short-chain fatty acid production in the rumen and lower microbial nitrogen synthesis were observed on day 7 after lipid inclusion compared to values found when steers were fed the control diet and on days 14 and 21 after lipid inclusion (P=0.041). Lipid inclusion in the diet decreased the population of protozoa on days 7, 14, and 21 (P<0.001). The abundances of R. albus and F. succinogenes were higher when steers were fed the control diet than the abundance observed on days 7, 14, and 21 after lipid inclusion in the diet (P<0.05). The first seven days of lipid diet inclusion are considered the most critical for ruminal adaptation, involving reductions in fibrolytic bacteria and changes in fermentation parameters. After 14 days the rumen showed signs of recovery and adaptation.
Development of selected performance, dressing and meat quality traits of Pekin ducks in relation to genotype and phytogenic feed additives
Authors: Lewko Lidia , Gornowicz Ewa , Kryza Artur , Adamski Marek 
Published: October 2024

Abstract
The aim of this study was to determine the effect of the genetic origin of ducks and the use of phytogenic additives in their feeding on performance indices, slaughter value and dressing percentage, as well as meat quality. The experimental material consisted of three genetic groups of Pekin ducks: Polish Pekin P-33 (P), Dworka D-11 (D) and Star 53 H.Y. (S). A 7-week experimental rearing of 216 commercial hybrids (3 × 72 animals) was carried out. Within each genetic line, three feeding groups were established, differing in the use of plant-based additives in the feed mixture (5% herb mixture or 5% black cumin seed). To assess slaughter value and meat quality of the ducks, 18 birds from each group were selected, including 9 males and 9 females. It was shown that the basic performance and slaughter value traits of Pekin ducks depend (p≤0.05) on their genetic origin. The most favourable (p≤0.05) rearing efficiency and slaughter value (body, carcass and meat weight) were found for commercial Star 53 HY ducks. Their breast muscles were characterized by higher (p≤0.05) cooking loss and lower (p≤0.05) collagen content. The genetic origin of the ducks was shown to influence (p≤0.05) economic efficiency of rearing, dressing percentage and chemical indicators of meat (except for water content in breast muscles) and some physical characteristics (pH24, cooking loss, colour lightness of leg muscles and electrical conductivity in breast muscles). Supplementation of the standard diet of ducks with phytogenic additives (mixture of herbs, black cumin seeds) at a level of 5% had (p≤0.05) a negative effect on body, carcass and meat weight, and a positive effect on the physical characteristics of meat related to water loss (pH24 and cooking loss).
Effect of the competitive exclusion culture on the growth, blood parameters, leg health, and innate immunity of broiler chickens
Authors: Żbikowski Artur , Michalczuk Monika , Pawłowski Karol , Adamczyk Krzysztof , Szafraniec Gustaw , Witkowska-Piłaszewicz Olga , Bień Damian , Damaziak Krzysztof , Łukasiewicz-Mierzejewska Monika , Dolka Beata , Kaukonen Eija , Szeleszczuk Piotr 
Published: October 2024

Abstract
The primary aim of poultry production is to obtain a high yield and quality end product. To reduce the risk of disease, many direct-fed microbial products have been developed. That appears to be an excellent tool for disease prevention.
We evaluated the influence of the commercial, competitive exclusion (CE) product, Broilact®, on the growth rate, hematology, serum biochemistry, and innate immunity in male ROSS-308 chickens, randomly divided into two groups (Broilact® treatment and control) raised for 42 days.
The birds’ body weight was determined at 1, 7, 14, 28, 35, and 42 days, and the blood samples were collected at days 22 and 42 of life. We observed lower mortality, better gait score, and higher final body weight in the Broilact® group. At day 22, birds from the treated group presented higher white blood cells counts (WBC) and T cytotoxic lymphocyte (CD8+) counts, higher total protein (TP) (fraction globulin and albumin), and lower triglyceride (TAG) and Ca2+ plasma concentrations. No differences were found in acute phase proteins (APPs). At day 42, only the K+ and Na+ concentrations were higher, while the IL-10 was lower in treated birds’ blood serum.
Our results indicate that treatment with one dose of the Broilact® product at day one of life has a beneficial influence, which improves the chickens’ performance, leg health and some serum enzymes activity, maintains electrolyte homeostasis, and influences leukocyte count with the rise of T CD8+ subpopulations.
Emotional fuctioning of people who experienced COVID-19 infection - the moderating role of gender
Authors: Gradowska Aleksandra , Szyls Patryk , Zadka Katarzyna , Ziora-Jakutowicz Karolina , Ławiński Michał 
Published: October 2024

Abstract
The COVID-19 pandemic had a significant impact on people’s psychological functioning, altering emotional lives by increasing anxiety and lowering mood. The purpose of the present study was to analyze the impact of COVID-19 infection on anxiety and mood, and to examine the role of gender and the significance of the number of infections. The study was based on a questionnaire and involved 210 participants (n = 101 who contracted COVID-19 and n = 109 who never experienced the disease). The Anxiety and Depression Scale (HADS), a tool with good psychometric properties, was used for assessment.
Gender differences were observed in levels of anxiety and depression. Men reported higher levels of anxiety and depression compared to women. Interestingly, those who had not experienced COVID-19 reported higher levels of depression. The study indicated a moderating role of gender in the relationship between anxiety, depression, and the number of infections. Experiencing an infection may reduce tension and possibly increase feelings of better coping and greater self-confidence, which is particularly significant for men, given the persistence of stereotypical attitudes towards male and female roles in society.
The individual experience of COVID-19 infection, coupled with the unpredictability of the body’s response, may foster a sense of uncertainty, exacerbating negative emotions. However, participants who had contracted the disease learned to cope with it and developed some immunity, which might explain the observed differences. This conclusion is important in the context of potential subsequent waves of the epidemic.
Unveiling the Subterranean Heritage: A Case Study of the Underground City of Kukës, Albania
Authors: Dobjani Etleva , Kristo Saimir 
Published: October 2024

Abstract
This paper investigates the viability of conserving and repurposing Kukës’ underground city, which was built as a system of civil defence tunnels during the communist era in Albania. Beneath contemporary Kukës, the underground complex, which houses administrative offices and hospitals, is an important but underutilized historical and architectural asset. This study assesses the architectural elements, historical value, and present state of the tunnels using an extensive methodology that includes expert interviews, public consultations, on-site inspections, and archival research. Results highlight the site’s dual significance as a historical landmark and a possible center for innovation and tourism.
The research offers strategic proposals for reviving the tunnels to strengthen the local economy, maintain cultural identity, and increase tourist attraction, drawing on foreign case studies. By promoting the inclusion of Kukës’ underground city in larger regional development projects and considering both community goals and pragmatic concerns, the article adds to the current conversations in urban planning and cultural preservation.
Sustaining and repurposing Kukës’ underground city has various benefits, including enhanced cultural tourism and economic resurgence that encourage sustainable growth, the paper points out. Still, challenges like deteriorating infrastructure, security concerns, and accessibility issues must be overcome. Urban planners and politicians can apply the research’s practical implications by using it to develop a model for cultural preservation that aligns with modern social and economic goals.
A sense of shared identity and legacy among the local community’s members is fostered by the initiative, which has significant social implications as well. The study has some drawbacks, including the need for significant funding and the potential for opposition from groups who do not recognize the advantages of these kinds of initiatives. To summarise, this research highlights the potential of adaptive reuse to bolster the sustainability and resilience of urban ecosystems by offering useful guidance and strategies that may be used to comparable initiatives worldwide.