bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor for advanced glycation end-products and ARDS prediction: a multicentre observational study
Authors: Jabaudon Matthieu , Berthelin Pauline , Pranal Thibaut , Roszyk Laurence , Godet Thomas , Faure Jean-Sébastien , Chabanne Russell , Eisenmann Nathanael , Lautrette Alexandre , Belville Corinne , Blondonnet Raiko , Cayot Sophie , Gillart Thierry , Pascal Julien , Skrzypczak Yvan , Souweine Bertrand , Blanchon Loic , Sapin Vincent , Pereira Bruno , Constantin Jean-Michel 
Published: February 2018

AbstractAcute respiratory distress syndrome (ARDS) prediction remains challenging despite available clinical scores. To assess soluble receptor for advanced glycation end-products (sRAGE), a marker of lung epithelial injury, as a predictor of ARDS in a high-risk population, adult patients with at least one ARDS risk factor upon admission to participating intensive care units (ICUs) were enrolled in a multicentre, prospective study between June 2014 and January 2015. Plasma sRAGE and endogenous secretory RAGE (esRAGE) were measured at baseline (ICU admission) and 24 hours later (day one). Four AGER candidate single nucleotide polymorphisms (SNPs) were also assayed because of previous reports of functionality (rs1800625, rs1800624, rs3134940, and rs2070600). The primary outcome was ARDS development within seven days. Of 500 patients enrolled, 464 patients were analysed, and 59 developed ARDS by day seven. Higher baseline and day one plasma sRAGE, but not esRAGE, were independently associated with increased ARDS risk. AGER SNP rs2070600 (Ser/Ser) was associated with increased ARDS risk and higher plasma sRAGE in this cohort, although confirmatory studies are needed to assess the role of AGER SNPs in ARDS prediction. These findings suggest that among at-risk ICU patients, higher plasma sRAGE may identify those who are more likely to develop ARDS.
Coordinated activation of a cluster of MMP genes in response to UVB radiation
Authors: Ujfaludi Zsuzsanna , Tuzesi Agota , Majoros Hajnalka , Rothler Balint , Pankotai Tibor , Boros Imre M. 
Published: February 2018

AbstractUltraviolet (UV) B radiation is a dangerous environmental stressor, which can lead to photoaging, inflammation, immune suppression and tumour formation. A recent report has shown the transcriptional activation of several skin-specific genes including matrix metalloproteases (MMPs) in response to UV irradiation. Here, we use a novel human keratinocyte model, HKerE6SFM, to demonstrate that UVB activates the transcription of most members of the 11q22.3 MMP gene cluster including MMP13, MMP12, MMP3, MMP1 and MMP10. Curiously, the expression of the well-characterized UVB-inducible MMP9, which is located outside of the cluster, remains unchanged. In accordance with the increased expression of the MMP gene cluster upon UVB irradiation, RNA polymerase II showed increased occupancy at their promoters following UVB irradiation. The results also demonstrate increased acetylated histone H3K9 levels at the promoters of the MMP13, MMP12, MMP3, MMP1 and MMP10 genes. These findings suggest a coordinated transcriptional activation of genes in the MMP cluster at 11q22.3 and that acetylation of histone H3 at lysine 9 has an important role in the UVB-dependent enhancement of transcription of MMP genes in this region.
Synthesis of light needles with tunable length and nearly constant irradiance
Authors: Martínez-Herrero Rosario , Maluenda David , Juvells Ignasi , Carnicer Artur 
Published: February 2018

AbstractWe introduce a new method for producing optical needles with tunable length and almost constant irradiance based on the evaluation of the on-axis power content of the light distribution at the focal area. According to theoretical considerations, we propose an adaptive modulating continuous function that presents a large derivative and a zero value jump at the entrance pupil of the focusing system. This distribution is displayed on liquid crystal devices using holographic techniques. In this way, a polarized input beam is shaped and subsequently focused using a high numerical aperture (NA) objective lens. As a result, needles with variable length and nearly constant irradiance are produced using conventional optics components. This procedure is experimentally demonstrated obtaining a 53λ-long and 0.8λ-wide needle.
Contemporary and historical oceanographic processes explain genetic connectivity in a Southwestern Atlantic coral
Authors: Peluso L. , Tascheri V. , Nunes F. L. D. , Castro C. B. , Pires D. O. , Zilberberg C. 
Published: February 2018

AbstractUnderstanding connectivity patterns has implications for evolutionary and ecological processes, as well as for proper conservation strategies. This study examined population genetic structure and migration patterns of the coralMussismilia hispida, one of the main reef builders in the Southwestern Atlantic Ocean. For this, 15 sites were sampled along its entire distributional range employing 10 microsatelliteloci.M.hispidawas divided into five genetically differentiated populations by Structure analysis. Population structure and migration estimates are consistent with present-day oceanographic current patterns, zones of upwelling and historical sea-level changes. The Central Region and Oceanic Islands populations had the highest genetic diversity, were possibly the main sources of migrants for other populations and presented mutual migrant exchange. This mutual exchange and the high diversity of Oceanic Islands, a peripherical population, is highly interesting and unexpected, but can be explained if these sites acted as refugia in past low sea-level stance. This is the first connectivity study in the region using hyper-variable markers and a fine sampling scale along 3,500 km. These results enlighten the population dynamics of an important reef building species and shows how oceanographic processes may act as barriers to dispersal for marine species, providing valuable information for management strategies.
Dose and slice thickness evaluation with nMAG gel dosimeters in computed tomography
Authors: Chuang Chun-Chao , Wu Jay 
Published: February 2018

AbstractComputed tomography (CT) has been widely used in clinical diagnosis. It is important to estimate radiation dose and perform image quality assurance procedures for CT scans. In this study, nMAG gel dosimeters were used to simultaneously measure the 300-mm weighted CT dose index (CTDI) and slice sensitivity profile (SSP) for multiple detector CT (MDCT). Magnetic resonance imaging (MRI) was performed on the irradiated gel to create R2‒dose response curves for the tube voltages of 120 and 140 kVp. The gel dosimeters were loaded in three home-made cylindrical phantoms to obtain CTDI100 and CTDI300. The full width at half maximum (FWHM) for 2, 5, 10, 14.4, and 38.4-mm slice thicknesses was measured and compared with the result obtained by radiochromic films. The difference in weighted CTDI100 obtained by the gel dosimeter and ionization chamber was less than 1%. The CTDI efficiency at 120 and 140 kVp was in the range of 80.1%–82.5%. The FWHM of SSP measured by the gel dosimeter matched very well with the nominal slice thickness. The use of nMAG gel dosimeters combined with the home-made cylindrical phantoms can provide 300-mm weighted CTDI and slice thickness information, showing potential for quality assurance and clinical applications in MDCT.
Evidence of thermophilisation and elevation-dependent warming during the Last Interglacial in the Italian Alps
Authors: Johnston V. E. , Borsato A. , Frisia S. , Spötl C. , Dublyansky Y. , Töchterle P. , Hellstrom J. C. , Bajo P. , Edwards R. L. , Cheng H. 
Published: February 2018

AbstractThermophilisation is the response of plants communities in mountainous areas to increasing temperatures, causing an upward migration of warm-adapted (thermophilic) species and consequently, the timberline. This greening, associated with warming, causes enhanced evapotranspiration that leads to intensification of the hydrological cycle, which is recorded by hydroclimate-sensitive archives, such as stalagmites and flowstones formed in caves. Understanding how hydroclimate manifests at high altitudes is important for predicting future water resources of many regions of Europe that rely on glaciers and snow accumulation. Using proxy data from three coeval speleothems (stalagmites and flowstone) from the Italian Alps, we reconstructed both the ecosystem and hydrological setting during the Last Interglacial (LIG); a warm period that may provide an analogue to a near-future climate scenario. Our speleothem proxy data, including calcite fabrics and the stable isotopes of calcite and fluid inclusions, indicate a +4.3 ± 1.6 °C temperature anomaly at ~2000 m a.s.l. for the peak LIG, with respect to present-day values (1961–1990). This anomaly is significantly higher than any low-altitude reconstructions for the LIG in Europe, implying elevation-dependent warming during the LIG. The enhanced warming at high altitudes must be accounted for when considering future climate adaption strategies in sensitive mountainous regions.
LC–MS/MS-based quantitative study of the acyl group- and site-selectivity of human sirtuins to acylated nucleosomes
Authors: Tanabe Kana , Liu Jiaan , Kato Daiki , Kurumizaka Hitoshi , Yamatsugu Kenzo , Kanai Motomu , Kawashima Shigehiro A. 
Published: February 2018

AbstractChromatin structure and gene expression are dynamically regulated by posttranslational modifications of histones. Recent advance in mass spectrometry has identified novel types of lysine acylations, such as butyrylation and malonylation, whose functions and regulations are likely different from those of acetylation. Sirtuins, nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases, catalyze various deacylations. However, it is poorly understood how distinct sirtuins regulate the histone acylation states of nucleosomes that have many lysine residues. Here, we provide mass spectrometry-based quantitative information about the acyl group- and site-selectivity of all human sirtuins on acylated nucleosomes. The acyl group- and site-selectivity of each sirtuin is unique to its subtype. Sirt5 exclusively removes negatively-charged acyl groups, while Sirt1/2/3/6/7 preferentially remove hydrophobic acyl groups; Sirt1 and Sirt3 selectively remove acetyl group more than butyryl group, whereas Sirt2 and Sirt6 showed the opposite selectivity. Investigating site-selectivity for active sirtuins revealed acylated lysines on H4 tails to be poor substrates and acylated H3K18 to be a good substrate. Furthermore, we found Sirt7 to be a robust deacylase of H3K36/37, and its activity reliant on nucleosome-binding at its C-terminal basic region. All together, our quantitative dataset provides a useful resource in understanding chromatin regulations by histone acylations.
Effects of water flow on submerged macrophyte-biofilm systems in constructed wetlands
Authors: Han Bing , Zhang Songhe , Wang Peifang , Wang Chao 
Published: February 2018

AbstractThe effects of water flow on the leaf-biofilm interface ofVallisneria natansandHydrilla verticillatawere investigated using artificial plants as the control. Water flow inhibited the growth of two species of submerged macrophytes, reduced oxygen concentrations in plant leaves and changed oxygen profiles at the leaf-biofilm interface. The results from confocal laser scanning microscopy and multifractal analysis showed that water flow reduced biofilm thickness, changed biofilm topographic characterization and increased the percentages of single colony-like biofilm patches. A cluster analysis revealed that the bacterial compositions in biofilms were determined mainly by substrate types and were different from those in sediments. However, water flow increased the bacterial diversity in biofilms in terms of operational taxonomic unit numbers and Shannon Indices. Our results indicated that water flow can be used to regulate the biomass, distribution and bacterial diversities of epiphytic biofilms in constructed wetlands dominated by submerged macrophytes.
Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery
Authors: Chandan Rajeet , Banerjee Rinti 
Published: February 2018

AbstractRecently, liposomes-microbubble conjugates have emerged as a promising ultrasound (US)-responsive platform for cancer therapeutics. However, these are limited by their size in terms of tumor penetration. Additionally, there have been no attempts to enhance the smartness of such conjugates which have been used only as passive carriers. The present study explores submicron sized (756 ± 180.0 nm), US-responsive, phosphatidylserine (PS)-based paclitaxel-liposomes-nanobubble conjugates (PSPLBC) with an additional pro-apoptotic effect towards enhanced anti-cancer efficacy and image-guidance. The developed PSPLBC underwent cavitation in response to US-trigger, exhibiting in vitro pulsatile release with a 10-fold increase in cellular internalization as compared to control. The PS-containing formulations were found to be pro-apoptotic and exhibited strong synergism between PS and paclitaxel (Combination Index, CI < 0.1). This resulted in significantly high anti-tumor efficacy both in vitro and in vivo conditions (98.3 ± 0.8% tumor growth inhibition, TGI). Significant reduction in tumor proliferation index and MVD, as well as significant increase in apoptosis, were observed for the treated tumor sections. Further, the intravenous (i.v.) administration of PSPLBC enhanced the tumor US-contrast by 2-fold as compared to SonoVue. These results, show the potential of PSPLBC as a promising non-invasive, pro-apoptotic, smart DDS for US-responsive, image-guided cancer therapeutics.
In Silico Non-Homologous End Joining Following Ion Induced DNA Double Strand Breaks Predicts That Repair Fidelity Depends on Break Density
Authors: Henthorn N. T. , Warmenhoven J. W. , Sotiropoulos M. , Mackay R. I. , Kirkby N. F. , Kirkby K. J. , Merchant M. J. 
Published: February 2018

AbstractThis work uses Monte Carlo simulations to investigate the dependence of residual and misrepaired double strand breaks (DSBs) at 24 hours on the initial damage pattern created during ion therapy. We present results from a nanometric DNA damage simulation coupled to a mechanistic model of Non-Homologous End Joining, capable of predicting the position, complexity, and repair of DSBs. The initial damage pattern is scored by calculating the average number of DSBs within 70 nm from every DSB. We show that this local DSB density, referred to as the cluster density, can linearly predict misrepair regardless of ion species. The models predict that the fraction of residual DSBs is constant, with 7.3% of DSBs left unrepaired following 24 hours of repair. Through simulation over a range of doses and linear energy transfer (LET) we derive simple correlations capable of predicting residual and misrepaired DSBs. These equations are applicable to ion therapy treatment planning where both dose and LET are scored. This is demonstrated by applying the correlations to an example of a clinical proton spread out Bragg peak. Here we see a considerable biological effect past the distal edge, dominated by residual DSBs.
Circulating miRNAs as diagnostic biomarkers for adolescent idiopathic scoliosis
Authors: García-Giménez José Luis , Rubio-Belmar Pedro Antonio , Peiró-Chova Lorena , Hervás David , González-Rodríguez Daymé , Ibañez-Cabellos José Santiago , Bas-Hermida Paloma , Mena-Mollá Salvador , García-López Eva María , Pallardó Federico V. , Bas Teresa 
Published: February 2018

AbstractThe aetiology of adolescent idiopathic scoliosis (AIS) has been linked to many factors, such as asymmetric growth, neuromuscular condition, bone strength and genetic background. Recently, epigenetic factors have been proposed as contributors of AIS physiopathology, but information about the molecular mechanisms and pathways involved is scarce. Regarding epigenetic factors, microRNAs (miRNAs) are molecules that contribute to gene expression modulation by regulating important cellular pathways. We herein used Next-Generation Sequencing to discover a series of circulating miRNAs detected in the blood samples of AIS patients, which yielded a unique miRNA biomarker signature that diagnoses AIS with high sensitivity and specificity. We propose that these miRNAs participate in the epigenetic control of signalling pathways by regulating osteoblast and osteoclast differentiation, thus modulating the genetic background of AIS patients. Our study yielded two relevant results: 1) evidence for the deregulated miRNAs that participate in osteoblast/osteoclast differentiation mechanisms in AIS; 2) this miRNA-signature can be potentially used as a clinical tool for molecular AIS diagnosis. Using miRNAs as biomarkers for AIS diagnostics is especially relevant since miRNAs can serve for early diagnoses and for evaluating the positive effects of applied therapies to therefore reduce the need of high-risk surgical interventions.
RAGE-aptamer attenuates deoxycorticosterone acetate/salt-induced renal injury in mice
Authors: Taguchi Kensei , Yamagishi Sho-ichi , Yokoro Miyuki , Ito Sakuya , Kodama Goh , Kaida Yusuke , Nakayama Yosuke , Ando Ryotaro , Yamada-Obara Nana , Asanuma Katsuhiko , Matsui Takanori , Higashimoto Yuichiro , Brooks Craig R. , Ueda Seiji , Okuda Seiya , Fukami Kei 
Published: February 2018

AbstractThe mineralocorticoid receptor (MR) and its downstream signaling play an important role in hypertensive renal injury. The interaction of advanced glycation end products (AGE) with their receptor (RAGE) is involved in the progression of renal disease. However, the pathological crosstalk between AGE–RAGE axis and MR system in kidney derangement remains unclear. We screened DNA-aptamer directed against RAGE (RAGE-apt) in vitro and examined its effects on renal injury in uninephrectomized deoxycorticosterone acetate (DOCA)/salt-induced hypertensive mice. RAGE, GTP-bound Rac-1 (Rac1), and MR were co-localized in the podocytes of DOCA mice. The deletion of RAGE gene significantly inhibited mesangial matrix expansion and tubulointerstitial fibrosis in DOCA mice, which was associated with the reduction of glomerular oxidative stress, MR, Rac1, and urinary albumin excretion (UAE) levels. RAGE-apt attenuated the increase in carboxymethyllysine (CML), RAGE, nitrotyrosine, Rac1, and MR levels in the kidneys and reduced UAE in DOCA mice. Aldosterone (Aldo) increased nitrotyrosine, CML, and RAGE gene expression in murine podocytes, whereas CML stimulated MR and Rac1 levels, which were blocked by RAGE-apt. The present study indicates the crosstalk between the AGE–RAGE axis and Aldo–MR system, suggesting that RAGE-apt may be a novel therapeutic tool for the treatment of MR-associated renal diseases.
Lingual Pressure as a Clinical Indicator of Swallowing Function in Parkinson's Disease
Authors: Pitts Laura L. , Morales Sarah , Stierwalt Julie A. G. 
Published: February 2018

PurposeSwallowing impairment, or dysphagia, is a known contributor to reduced quality of life, pneumonia, and mortality in Parkinson's disease (PD). However, the contribution of tongue dysfunction, specifically inadequate pressure generation, to dysphagia in PD remains unclear. Our purpose was to determine whether lingual pressures in PD are (a) reduced, (b) reflect medication state, or are (c) consistent with self-reported diet and swallowing function.MethodTwenty-eight persons with idiopathic PD (PwPD) and 28 age- and sex-matched controls completed lingual pressure tasks with the Iowa Oral Performance Instrument. PwPD were tested during practically defined ON and OFF dopaminergic medication states. Participants were also stratified into three sex- and age-matched cohorts (7 men, 5 women): (a) controls, (b) PwPD without self-reported dysphagia symptoms or diet restrictions, and (c) PwPD with self-reported dysphagia symptoms with or without diet restrictions.ResultsPwPD exhibited reduced tongue strength and used elevated proportions of tongue strength during swallowing compared with controls (p< .05) without an effect of medication state (p> .05). Reduced tongue strength distinguished PwPD with self-reported dysphagia symptoms from PwPD without reported symptoms or diet restrictions (p= .045) and controls (p= .002).ConclusionTongue strength was significantly reduced in PwPD and did not differ by medication state. Tongue strength differentiated between PwPD with and without self-reported swallowing symptoms. Therefore, measures of tongue strength and swallowing pressures may serve as clinical indicators for further dysphagia evaluation and may promote early diagnosis and management of dysphagia in PD.
Q‐switched and mode‐locked ytterbium‐doped fibre lasers with Sb2 Te3 topological insulator saturable absorber
Authors: Al‐Masoodi Ahmed H.H. , Ahmad Fauzan , Ahmed Mahmoud H.M. , Al‐Masoodi Abtisam H.H. , Alani Ibrahim A.M. , Arof Hamzah , Harun Sulaiman Wadi 
Published: February 2018

Q‐switched and mode‐locked fibre lasers have been successfully demonstrated in ytterbium‐doped fibre laser cavity by taking an advantage of the optical absorption of antimony telluride (Sb2 Te3) material. The Sb2 Te3 was embedded into polyvinyl alcohol to function as a saturable absorber (SA). A Q‐switching pulses train was obtained by incorporating the SA into the laser ring cavity configured with 3 dB coupler. The Q‐switching pulse repetition rate increases from 24.4 to 55 kHz as the pump power is increased from the threshold of 75.4–96.2 mW. The maximum pulse energy of 252.6 nJ is obtained at 82.3 mW pump power. On the other hand, by changing the output coupler to 10 dB coupler, a stable self‐started mode‐locking operation was then generated at pump power range from 47.8 to 89.4 mW with a fixed repetition rate of 24.2 MHz. At 89.4 mW pump power, the maximum output power and pulse energy are obtained to be around 18.6 mW and 0.8 nJ, respectively. The authors results display that the Sb2 Te3 material could also be developed as an effective SA for both Q‐switched and mode‐locked fibre lasers.
Towards an improvement in the spectral description of central stars of planetary nebulae
Authors: Weidmann W. , Gamen R. , Mast D. , Fariña C. , Gimeno G. , Schmidt E. O. , Ashley R. P. , Peralta de Arriba L. , Sowicka P. , Ordonez-Etxeberria I. 
Published: February 2018

Context. There are more than 3000 known Galactic planetary nebulae, but only 492 central stars of Galactic planetary nebulae (CSPN) have known spectral types. It is vital to increase this number in order to have reliable statistics, which will lead to an increase of our understanding of these amazing objects.
Aims. We aim to contribute to the knowledge of central stars of planetary nebulae and stellar evolution.
Methods. This observational study is based on Gemini Multi-Object Spectrographs (GMOS) and with the Intermediate Dispersion Spectrograph (IDS) at the Isaac Newton Telescope spectra of 78 CSPN. The objects were selected because they did not have any previous classification, or the present classification is ambiguous. These new high quality spectra allowed us to identify the key stellar lines for determining spectral classification in the Morgan-Keenan (MK) system.
Results. We have acquired optical spectra of a large sample of CSPN. From the observed targets, 50 are classified here for the first time while for 28 the existing classifications have been improved. In seven objects we have identified a P-Cygni profile at the He I lines. Six of these CSPN are late O-type. The vast majority of the stars in the sample exhibit an absorption-type spectrum, and in one case we have found wide emission lines typical of [WR] stars. We give a complementary, and preliminary, classification criterion to obtain the sub-type of the O(H)-type CSPN. Finally, we give a more realistic value of the proportion of CSPN that are rich or poor in hydrogen.