Differences in the Distribution of Peripheral Blood Leukocyte and Lymphocyte Subsets in Pulmonary Tuberculosis Patients
Authors: Carneiro Valdirene Leão , Donato dos Santos Rosalina Guedes , Sampaio Geraldo Pedral , Casela Marilda , Netto Eduardo Martins , Nascimento Roberto José Meyer , Freire Songeli Menezes 
Published: November 2017

<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>PT-BR</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> <m:mathPr> <m:mathFont m:val="Cambria Math" /> <m:brkBin m:val="before" /> <m:brkBinSub m:val=" " /> <m:smallFrac m:val="off" /> <m:dispDef /> <m:lMargin m:val="0" /> <m:rMargin m:val="0" /> <m:defJc m:val="centerGroup" /> <m:wrapIndent m:val="1440" /> <m:intLim m:val="subSup" /> <m:naryLim m:val="undOvr" /> </m:mathPr></w:WordDocument> </xml><![endif]--> <p class="MsoNormal" style="margin-bottom: .0001pt; text-align: justify; line-height: normal; mso-layout-grid-align: none; text-autospace: none;"><strong><em><span style="font-size: 8.0pt; font-family: Calibri-BoldItalic; mso-bidi-font-family: Calibri-BoldItalic;">Introduction: </span></em></strong><em><span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">It has been suggested that individuals exposed to Mycobacterium tuberculosis can eliminate the infection through the innate immune response without the participation of the acquired immunity. However, T helper type 1 immune response is critical to control persistent infection. </span></em><strong><em><span style="font-size: 8.0pt; font-family: Calibri-BoldItalic; mso-bidi-font-family: Calibri-BoldItalic;">Objective</span></em></strong><em><span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">: The purpose of this study was to evaluate the distribution of leukocyte and lymphocyte subsets in peripheral blood of individuals with pulmonary tuberculosis (TB) and positive Tuberculin Skin Test (TST). </span></em><strong><em><span style="font-size: 8.0pt; font-family: Calibri-BoldItalic; mso-bidi-font-family: Calibri-BoldItalic;">Methodology</span></em></strong><em><span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">: In order to achieve that goal, we have collected blood sample from thirty-four treatment-naïve TB patients, fifty TST-positive and forty-one TST-negative individuals. </span></em><strong><em><span style="font-size: 8.0pt; font-family: Calibri-BoldItalic; mso-bidi-font-family: Calibri-BoldItalic;">Results: </span></em></strong><em><span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">The evaluation has shown a significant reduction in the number of total lymphocytes, B cells, CD4+ and CD8+ T cells and percentage increase of NK cells, neutrophils and monocytes in TB patients, when compared to TSTpositive and TST-negative individuals. There was no statistical difference for leucocyte and lymphocyte subsets between TST-positive and TST-negative groups. Peripheral blood white cell counts change significantly at diagnosis. </span></em><strong><span style="font-size: 8.0pt; font-family: Calibri-Bold; mso-bidi-font-family: Calibri-Bold;">Conclusion: </span></strong><em><span style="font-size: 8.0pt; font-family: ";Calibri-Italic";,";sans-serif";; mso-bidi-font-family: Calibri-Italic;">The quantification of these cells may support the diagnosis and monitoring of patients with pulmonary tuberculosis.</span></em></p> <!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" DefUnhideWhenUsed="true" DefSemiHidden="true" DefQFormat="false" DefPriority="99" LatentStyleCount="267"> <w:LsdException Locked="false" Priority="0" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="Normal" /> <w:LsdException Locked="false" Priority="9" SemiHidden="false" UnhideWhenUsed="false" QFormat="true" Name="heading 1" /> <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 2" /> <w:LsdException Locked="false" Priority="9" 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