bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA sequencing of esophageal adenocarcinomas identifies novel fusion transcripts, including NPC1‐MELK, arising from a complex chromosomal rearrangement
Authors: Wang Zhixiong , Cheng Yulan , Abraham John M. , Yan Rong , Liu Xi , Chen Wei , Ibrahim Sariat , Schroth Gary P. , Ke Xiquan , He Yulong , Meltzer Stephen J. 
Published: June 2017

BACKGROUNDStudies of chromosomal rearrangements and fusion transcripts have elucidated mechanisms of tumorigenesis and led to targeted cancer therapies. This study was aimed at identifying novel fusion transcripts in esophageal adenocarcinoma (EAC).METHODSTo identify new fusion transcripts associated with EAC, targeted RNA sequencing and polymerase chain reaction (PCR) verification were performed in 40 EACs and matched nonmalignant specimens from the same patients. Genomic PCR and Sanger sequencing were performed to find the breakpoint of fusion genes.RESULTSFive novel in‐frame fusion transcripts were identified and verified in 40 EACs and in a validation cohort of 15 additional EACs (55 patients in all): fibroblast growth factor receptor 2 (FGFR2)–GRB2‐associated binding protein 2 (GAB2) in 2 of 55 or 3.6%, Niemann‐Pick C1 (NPC1)–maternal embryonic leucine zipper kinase (MELK) in 2 of 55 or 3.6%, ubiquitin‐specific peptidase 54 (USP54)–calcium/calmodulin dependent protein kinase II γ (CAMK2G) in 2 of 55 or 3.6%, megakaryoblastic leukemia (translocation) 1 (MKL1)–fibulin 1 (FBLN1) in 1 of 55 or 1.8%, and CCR4‐NOT transcription complex subunit 2 (CNOT2)–chromosome 12 open reading frame 49 (C12orf49) in 1 of 55 or 1.8%. A genomic analysis indicated that NPC1‐MELK arose from a complex interchromosomal translocation event involving chromosomes 18, 3, and 9 with 3 rearrangement points, and this was consistent with chromoplexy.CONCLUSIONSThese data indicate that fusion transcripts occur at a stable frequency in EAC. Furthermore, our results indicate that chromoplexy is an underlying mechanism that generates fusion transcripts in EAC. These and other fusion transcripts merit further study as diagnostic markers and potential therapeutic targets in EAC. Cancer 2017;123:3916‐24. © 2017 American Cancer Society.
Change in attachment states of mind of women with binge‐eating disorder
Authors: Maxwell Hilary , Tasca Giorgio A. , Grenon Renee , Ritchie Kerri , Bissada Hany , Balfour Louise 
Published: June 2017

Insecure and unresolved/disorganized attachment states of mind may impact affect regulation and interpersonal functioning that contribute to binge eating in women with binge‐eating disorder (BED). Group psychological treatment may facilitate changes from insecure to secure and from unresolved–disorganized to non‐unresolved/disorganized attachment states of mind. This study used attachment theory to understand better the psychopathology of BED and co‐morbid overweight status and to understand better the treatment response of patients with BED who receive group psychotherapy. Women with BED attended group psychodynamic interpersonal psychotherapy and completed the Adult Attachment Interview pretreatment and 6 months posttreatment. Matched samples of overweight women without BED and normal‐weight women without BED completed the Adult Attachment Interview at 1 time point. Women with BED had significantly higher rates of preoccupied and unresolved/disorganized attachment states of mind compared to normal‐weight women without BED and had similar rates of insecure and unresolved/disorganized attachment states of mind compared to overweight women without BED. Of the women with BED who had an insecure and/or unresolved/disorganized attachment states of mind at pretreatment, about 60% demonstrated clinically relevant changes to secure and to non‐unresolved/disorganized states of mind at 6 months post group psychodynamic interpersonal psychotherapy. Results indicated that some women with BED may benefit from interventions that help them regulate hyperactivated affect and create coherent narratives. Both women with BED and overweight women without BED may benefit from treatments that help them develop more adaptive affect regulation strategies related to unresolved/disorganized attachment states of mind.
A survey on deployment techniques, localization algorithms, and research challenges for underwater acoustic sensor networks
Authors: Tuna Gurkan , Gungor V. Cagri 
Published: June 2017

SummaryIn recent years, wireless sensor networks (WSNs) have attracted the attention of both the research community and the industry, and this has eventually lead to the widespread use of WSNs in various applications. The significant advancements in WSNs and the advantages brought by WSNs have also enabled the rapid development of underwater acoustic sensor networks (UASNs). In UASNs, in addition to deployment, determining the locations of underwater sensor nodes after they have been deployed is important since it plays a critical role in many applications. Various localization techniques have been proposed for UASNs, and each one is suitable for specific scenarios and has unique challenges. In this paper, after presenting an overview of potential UASN applications, a survey of the deployment techniques and localization algorithms for UASNs has been presented based on their major advantages and disadvantages. Finally, research challenges and open research issues of UASNs have been discussed to provide an insight into future research opportunities.
A phase III randomized controlled study on the efficacy and improved bowel function of prolonged‐release (PR) oxycodone‐naloxone (up to 160/80 mg daily) vs oxycodone PR
Authors: Dupoiron D. , Stachowiak A. , Loewenstein O. , Ellery A. , Kremers W. , Bosse B. , Hopp M. 
Published: June 2017

AbstractBackgroundOxycodone/naloxone (OXN PR) is a prolonged‐release formulation containing oxycodone and naloxone in a 2:1 ratio. This study aimed to evaluate the tolerability and efficacy of doses up to OXN160/80 mg PR compared with oxycodone prolonged‐release formulation (OxyPR) in a randomised controlled trial.MethodsTwo hundred and forty‐three patients were randomised to treatment with OXN PR (n = 123) or OxyPR (n = 120) during the 5‐week double‐blind study. Measured were: opioid‐induced constipation [bowel function index score (BFI)]; analgesic efficacy (NRS 0–10); daily laxative rescue medication use; rescue medication use, and the number of complete spontaneous bowel movements (CSBMs) per week. A subanalysis was conducted in cancer patients.ResultsGreater reductions in mean BFI scores were reported for the OXN PR group compared with OxyPR from Week 1 onwards; at Week 5 the mean change from baseline was −32.5 versus −14.2. Average 24‐h pain scores were low and remained stable in the range 3–4 in both treatment groups. Analgesic rescue medication use was similar between the groups. Patients receiving OXN PR used significantly lower mean daily doses of laxative rescue medication than those receiving OxyPR (P = 0.006). The number of CSBM in the OXN PR group approximately doubled compared with a 25% decrease in the OxyPR group. Comparable results to the total study population were reported in the cancer patient subgroup.ConclusionsOXN PR in daily doses of up to 160/80 mg significantly improves bowel function compared with equivalent doses of OxyPR while still providing comparable analgesic efficacy.SignificanceEffective analgesia can be achieved using oxycodone/naloxone PR up to 160/80 mg daily without compromising bowel function. A similar outcome was reported in cancer and non‐cancer patients.
Response and survival benefit with chemoimmunotherapy in Epstein‐Barr virus‐positive diffuse large B‐cell lymphoma
Authors: Beltran Brady E. , Quiñones Pilar , Morales Domingo , Malaga Jose M. , Chavez Julio C. , Sotomayor Eduardo M. , Castillo Jorge J. 
Published: June 2017

AbstractEpstein‐Barr virus (EBV)‐positive diffuse large B‐cell lymphoma (DLBCL) is a haematologic malignancy with poor prognosis when treated with chemotherapy. We evaluated response and survival benefits of chemoimmunotherapy in EBV‐positive DLBCL patients. A total of 117 DLBCL patients were included in our retrospective analysis; 33 were EBV‐positive (17 treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone [R‐CHOP] and 16 with CHOP), and 84 were EBV‐negative (all treated with R‐CHOP). The outcomes of interest were complete response (CR) and overall survival (OS) in EBV‐positive DLBCL patients (R‐CHOP versus CHOP) and in DLBCL patients treated with R‐CHOP (EBV‐positive vs EBV‐negative). There were no differences in the clinical characteristics between EBV‐positive and EBV‐negative DLBCL patients. Among EBV‐positive DLBCL patients, R‐CHOP was associated with higher odds of CR (OR 3.14, 95% CI 0.75‐13.2;P = .10) and better OS (hazard ratio 0.30, 95% confidence interval [CI] 0.09‐0.94;P = .04). There were no differences in CR rate (OR 0.52, 95% CI 0.18‐1.56;P = .25) or OS (hazard ratio 0.93, 95% CI 0.32‐2.67;P = .89) between EBV‐positive and EBV‐negative DLBCL patients treated with R‐CHOP. Based on our study, the addition of rituximab to CHOP is associated with improved response and survival in EBV‐positive DLBCL patients. Epstein‐Barr virus status does not seem to affect response or survival in DLBCL patients treated with R‐CHOP.
Necessity and feasibility of improving mental health services in China: A systematic qualitative review
Authors: Zhao Xudong , Liu Liang , Hu Chengping , Chen Fazhan , Sun Xirong 
Published: June 2017

SummaryIt has been nearly 40 years since the reform and opening up of Mainland China. The mental health services system has developed rapidly as a part of the profound socioeconomic changes that ensued. However, its development has not been as substantial as other areas of medical care. For the current qualitative systematic review, we searched databases, including China Biology Medicine disc, Weipu, China National Knowledge Infrastructure, Wanfang digital periodical full text data, China's important newspaper full text database, China Statistical Yearbook database, etc. The content of primary research, literature, and policy papers about the evolution and development of Chinese mental health services was systemically reviewed and analysed by using thematic analysis. Two main themes relative to the necessity and feasibility of reforming the current mental health services system emerged. We discuss 5 corresponding subthemes under the umbrella of the necessity of improving the current treatment, rehabilitation, prevention, and service systems and 7 requirements for the feasibility of reforming the current system. We conclude that as the development of the Chinese economy and the spirit of humanistic care continue, the improvement and reformation of the mental health services system are both necessary and feasible.
Sedimentation of overbank floods in the confined complex channel–floodplain system of the Lower Yellow River, China
Authors: Zhang Min , Huang He Qing , Carling Paul A. , Zhang Mingwu 
Published: June 2017

AbstractThe channel boundary conditions along the Lower Yellow River (LYR) have been altered significantly since the 1950s with the continual reinforcement and construction of both main and secondary dykes and river training works. To evaluate how the confined complex channel–floodplain system of the LYR responds to floods, this study presents a detailed investigation of the relationship between the tempo‐spatial distribution of sedimentation/erosion and overbank floods occurred in the LYR. For large overbank floods, we found that when the sediment transport coefficient (ratio of sediment concentration of flow to flow discharge) is less than 0.034, the bankfull channel is subject to significant erosion, whereas the main and secondary floodplains both accumulate sediment. The amount of sediment deposited on the main and secondary floodplains is closely related to the ratio of peak discharge to bankfull discharge, volume of water flowing over the floodplains, and sediment concentration of overbank flow, whereas the degree of erosion in the bankfull channel is related to the amount of sediment deposited on the main and secondary floodplains, water volume, and sediment load in flood season. The significant increase in erosion in the bankfull channel is due to the construction of the main and secondary dykes and river training works, which are largely in a wide and narrow alternated pattern along the LYR such that the water flowing over wider floodplains returns to the channel downstream after it drops sediment. For small overbank floods, the bankfull channel is subject to erosion when the sediment transport coefficient is less than 0.028, whereas the amount of sediment deposited on the secondary floodplain is associated closely with the sediment concentration of flow. Over the entire length of the LYR, the situation of erosion in the bankfull channel and sediment deposition on the main and secondary floodplains occurred mainly in the upper reach of the LYR, in which a channel wandering in planform has been well developed.
A single‐center retrospective study of pregnancy outcomes after emergency cerclage for cervical insufficiency
Authors: Wang Sheng , Feng Ling 
Published: June 2017

AbstractObjectiveTo compare maternal and perinatal outcomes after emergency cerclage with those after elective cerclage.MethodsIn a retrospective review, data were assessed from women with a viable singleton pregnancy who underwent elective or emergency cerclage for cervical insufficiency at the Tongji Hospital, Wuhan, China, between January 2010 and July 2015. Subgroup analyses based on cervical length (CL; ≤15, 15–25, and 25–30 mm) were also conducted among women undergoing emergency cerclage.ResultsIn total, 68 women underwent elective cerclage and 53 underwent emergency cerclage. The suture‐to‐delivery interval was significantly longer in the elective group (19.17 ± 5.86 weeks) than in the emergency group (11.29 ± 7.27 weeks; P<0.001). There was no difference between the elective and emergency groups in mean pregnancy length at delivery, frequency of Apgar score below 7 at 5 minutes (live births only), or birth weight (live births only). An inverse trend in the degree of CL shortening with pregnancy outcomes was observed; women with a CL of 25–30 mm had the best outcomes.ConclusionPregnancy outcomes were similar after emergency and elective cerclage. There was an inverse trend in the degree of CL shortening with pregnancy outcomes in the emergency cerclage group, with better outcomes observed for women with longer CL.
Transferrin‐conjugated magnetic dextran‐spermine nanoparticles for targeted drug transport across blood‐brain barrier
Authors: Ghadiri Maryam , Vasheghani‐Farahani Ebrahim , Atyabi Fatemeh , Kobarfard Farzad , Mohamadyar‐Toupkanlou Farzaneh , Hosseinkhani Hossein 
Published: June 2017

AbstractApplication of many vital hydrophilic medicines have been restricted by blood–brain barrier (BBB) for treatment of brain diseases. In this study, a targeted drug delivery system based on dextran‐spermine biopolymer was developed for drug transport across BBB. Drug loaded magnetic dextran‐spermine nanoparticles (DS‐NPs) were prepared via ionic gelation followed by transferrin (Tf) conjugation as targeting moiety. The characteristics of Tf conjugated nanoparticles (TDS‐NPs) were analyzed by different methods and their cytotoxicity effects on U87MG cells were tested. The superparamagnetic characteristic of TDS‐NPs was verified by vibration simple magnetometer. Capecitabine loaded TDS‐NPs exhibited pH‐sensitive release behavior with enhanced cytotoxicity against U87MG cells, compared to DS‐NPs and free capecitabine. Prussian‐blue staining and TEM‐imaging showed the significant cellular uptake of TDS‐NPs. Furthermore, a remarkable increase of Fe concentrations in brain was observed following their biodistribution and histological studiesin vivo,after 1 and 7 days of post‐injection. Enhanced drug transport across BBB and pH‐triggered cellular uptake of TDS‐NPs indicated that these theranostic nanocarriers are promising candidate for the brain malignance treatment. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2851–2864, 2017.
Ultrasound‐assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell biowaste and study of mechanical and biological properties for orthopedic applications
Authors: Ingole Vijay H. , Hany Hussein Kamal , Kashale Anil A. , Ghule Kalyani , Vuherer Tomaž , Kokol Vanja , Chang Jia‐Yaw , Ling Yong‐Chien , Vinchurkar Aruna , Dhakal Hom N. , Ghule Anil V. 
Published: June 2017

AbstractNanostructured hydroxyapatite (HAp) is the most favorable candidate biomaterial for bone tissue engineering because of its bioactive and osteoconductive properties. Herein, we report for the first time ultrasound‐assisted facile and economic approach for the synthesis of nanocrystalline hydroxyapatite (Ca10(PO4)6(OH)2) using recycled eggshell biowaste referred as EHAp. The process involves the reaction of eggshell biowaste as a source of calcium and ammonium dihydrogen orthophosphate as a phosphate source. Ultrasound‐mediated chemical synthesis of hydroxyapatite (HAp) is also carried out using similar approach wherein commercially available calcium hydroxide and ammonium dihydrogen orthophosphate were used as calcium and phosphate precursors, respectively and referred as CHAp for better comparison. The prepared materials were characterized by X‐ray diffraction, field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and Raman spectroscopy to determine crystal structure, particle morphology, and the presence of chemical functional groups. The nanocrystalline EHAp and CHAp were observed to have spherical morphology with uniform size distribution. Furthermore, mechanical properties such as Vickers hardness, fracture toughness, and compression tests have been studied of the EHAp and CHAp samples showing promising results. Mechanical properties show the influence of calcination at 600°C EHAp and CHAp material. After calcination, in the case of EHAp material an average hardness, mechanical strength, elastic modulus, and fracture toughness were found 552 MPa, 46.6 MPa, 2824 MPa, and 3.85 MPa m1/2, respectively, while in the case of CHAp 618 MPa, 47.5 MPa, 2071 MPa, and 3.13 MPa m1/2. In vitro cell studies revealed that the EHAp and CHAp nanoparticles significantly increased the attachment and proliferation of the hFOB cells. Here, we showed that EHAp and CHAp provide promising biocompatible materials that do not affect the cell viability and proliferation with enhancing the osteogenic activity of osteoblasts. Moreover, hFOB cells are found to express Osteocalcin, Osteopontin, Collagen I, Osteonectin, BMP‐2 on the EHAp and CHAp bone graft. This study demonstrates the formation of pure nanocrystalline HAp with promising properties justifying the fact that the eggshell biowaste could be successfully used for the synthesis of HAp with good mechanical and osteogenic properties. These findings may have significant implications for designing of biomaterial for use in orthopedic tissue regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2935–2947, 2017.
Muscle mass as a target to reduce fatigue in patients with advanced cancer
Authors: Neefjes Elisabeth C.W. , van den Hurk Renske M. , Blauwhoff‐Buskermolen Susanne , van der Vorst Maurice J.D.L. , Becker‐Commissaris Annemarie , de van der Schueren Marian A.E. , Buffart Laurien M. , Verheul Henk M.W. 
Published: June 2017

AbstractBackgroundCancer‐related fatigue (CRF) reduces quality of life and the activity level of patients with cancer. Cancer related fatigue can be reduced by exercise interventions that may concurrently increase muscle mass. We hypothesized that low muscle mass is directly related to higher CRF.MethodsA total of 233 patients with advanced cancer starting palliative chemotherapy for lung, colorectal, breast, or prostate cancer were studied. The skeletal muscle index (SMI) was calculated as the patient's muscle mass on level L3 or T4 of a computed tomography scan, adjusted for height. Fatigue was assessed with the Functional Assessment of Chronic Illness Therapy‐fatigue questionnaire (cut‐off for fatigue <34). Multiple linear regression analyses were conducted to study the association between SMI and CRF adjusting for relevant confounders.ResultsIn this group of patients with advanced cancer, the median fatigue score was 36 (interquartile range 26–44). A higher SMI on level L3 was significantly associated with less CRF for men (B 0.447, P 0.004) but not for women (B − 0.401, P 0.090). No association between SMI on level T4 and the Functional Assessment of Chronic Illness Therapy‐fatigue score was found (n = 82).ConclusionsThe association between SMI and CRF may lead to the suggestion that male patients may be able to reduce fatigue by exercise interventions aiming at an increased muscle mass. In women with advanced cancer, CRF is more influenced by other causes, because it is not significantly related to muscle mass. To further reduce CRF in both men and women with cancer, multifactorial assessments need to be performed in order to develop effective treatment strategies.
Molecular interactions of dioxins and DLCs with the xenosensors (PXR and CAR): An in silico risk assessment approach
Authors: Verma Garima , Khan Mohemmed Faraz , Shaquiquzzaman Mohammad , Akhtar Wasim , Akhter Mymoona , Hasan Syed Misbahul , Alam Mohammad Mumtaz 
Published: June 2017

AbstractDioxins and dioxin‐like compounds (DLCs) are known to cause endocrine disruption in humans and animals. Being lipophilic xenobiotic chemicals, they can be easily absorbed into the biological system from the surrounding environments, thereby causing various health dysfunctions. In the present study, a total of 100 dioxins and DLCs were taken, and their binding pattern was assessed with the xenosensors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) in comparison with the corresponding known inhibitors and a well‐studied endocrine disrupting xenobiotic, bisphenol A (BPA). The nuclear receptors CAR and PXR are known to play a significant role in handling potential toxins by coordinating cellular transport and metabolic functions of the same. Among different endocrine‐disrupting chemicals used in the present study, DLCs (PCDFs and PCBs) elicited better interactions in comparison with the parent dioxin (polychlorinated dibenzodioxins) compounds. On comparing D scores of all the compounds against both the receptors, PCDF 8‐hydroxy‐3,4‐dichlorodibenzofuran (8‐OH‐DCDF) and PCB tetrachlorobenzyltoluene (TCBT) exhibited significant molecular interactions against PXR (−7.633 kcal mol−1) and CAR (−8.389 kcal mol−1), respectively. Predominant interactions were found to be H‐bonding, π‐π stacking, hydrophobic, polar, and van der Waals. By contrast, BPA and some natural ligands tested in this study showed lower binding affinities with these receptors than certain DLCs reported herein, ie, certain DLCs might be more toxic than the proven toxic agent, BPA. Such studies play a pivotal role in the risk assessment of exposure to dioxins and DLCs on human health.