bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the TNF‐α receptor type 1 on prostate carcinogenesis in knockout mice
Authors: Galheigo Maria Raquel Unterkircher , Cruz Amanda Rodrigues , Cabral Ágata Silva , Faria Paulo Rogério , Cordeiro Renato Simões , Silva Marcelo José Barbosa , Tomiosso Tatiana Carla , Gonçalves Bianca Fachim , Pinto‐Fochi Maria Etelvina , Taboga Sebastião Roberto , Góes Rejane Maira , Ribeiro Daniele Lisboa 
Published: March 2016

BACKGROUNDTNF‐α is a key cytokine involved in prostate carcinogenesis and is mediated by the TNF‐α receptor type 1 (TNFR‐1). This receptor triggers two opposite pathways: cell death or cell survival and presents a protective or stimulator role in cancer. Thus, the purpose of this study was to evaluate the role of TNF signaling in chemically induced prostate carcinogenesis in mice.METHODSC57bl/6 wild type (WT) and p55 TNFR‐1 knockout mice (KO) were treated with mineral oil (control) or N‐methyl N‐nitrosurea (MNU) in association with testosterone (MNU+T, single injection of 40 mg/kg and weekly injection 2 mg/kg, respectively) over the course of 6 months. After this induction period, prostate samples were processed for histological and biochemical analysis.RESULTSMNU+T treatment led to the development of prostate intraepithelial neoplasia (PIN) and adenocarcinoma (PCa) in both WT and KO animals; however, the incidence of PCa was lower in KO group than in WT. Cell proliferation analysis showed that PCNA levels were significantly lower in the KO group, even after carcinogenesis induction. Furthermore, the prostate of KO animals had lower levels of p65 and p‐mTOR after treatment with MNU+T than WT. There was also a decrease in prostate androgen receptor levels after induction of carcinogenesis in both KO and WT mice. Regarding the extracellular matrix in the prostate, KO mice had higher levels of fibronectin and lower levels of matrix metalloproteinase 2 (MMP2) after carcinogenesis. Finally, there was a similar increase in apoptosis in both groups after carcinogenesis, indicating that the TNAFr1 pathway in prostate carcinogenesis presented proliferative, and not apoptotic, stimuli.CONCLUSIONSTNF‐α, through its receptor TNFR‐1, promoted cell proliferation and cell survival in prostate by activation of the AKT/mTOR and NFKB pathway, which stimulated prostate carcinogenesis in chemically induced mice. Prostate 76: 917–926, 2016. © 2016 Wiley Periodicals, Inc.
Interplay between binding affinity and kinetics in protein–protein interactions
Authors: Cao Huaiqing , Huang Yongqi , Liu Zhirong 
Published: March 2016

ABSTRACTTo clarify the interplay between the binding affinity and kinetics of protein–protein interactions, and the possible role of intrinsically disordered proteins in such interactions, molecular simulations were carried out on 20 protein complexes. With bias potential and reweighting techniques, the free energy profiles were obtained under physiological affinities, which showed that the bound‐state valley is deep with a barrier height of 12 − 33 RT. From the dependence of the affinity on interface interactions, the entropic contribution to the binding affinity is approximated to be proportional to the interface area. The extracted dissociation rates based on the Arrhenius law correlate reasonably well with the experimental values (Pearson correlation coefficient R = 0.79). For each protein complex, a linear free energy relationship between binding affinity and the dissociation rate was confirmed, but the distribution of the slopes for intrinsically disordered proteins showed no essential difference with that observed for ordered proteins. A comparison with protein folding was also performed. Proteins 2016; 84:920–933. © 2016 Wiley Periodicals, Inc.
Moderating Role of Acculturation in a Mediation Model of Work–Family Conflict among Chinese Immigrants in New Zealand
Authors: Shang Sudong , O'Driscoll Michael P. , Roche Maree 
Published: March 2016

AbstractThis study examined the antecedents of work–family conflict (WFC) and the mediation effects of WFC on well‐being consequences among Chinese immigrants to New Zealand, along with the moderating role of acculturation. Four types of WFC were explored: time‐based and strain‐based work interference with family, and time‐based and strain‐based family interference with work. Data were collected from 577 Chinese immigrants in New Zealand, who had full‐time or part‐time work and lived with family members in New Zealand. The four types of WFC were differentially related to the antecedents and well‐being consequences, providing some evidence that both Chinese and New Zealand cultures may exert influences on Chinese immigrants' experiences of WFC. Both directions of WFC (work interference with family, and family interference with work) were related to job satisfaction and family satisfaction, and strain‐based WFC influenced their well‐being more than time‐based WFC. Most importantly, we found immigrants who were proficient in English perceived greater WFC and psychological strain. Copyright © 2016 John Wiley & Sons, Ltd.
The politics of deer farming in North Carolina—Lessons learned
Authors: Brown Robert D. 
Published: March 2016

ABSTRACTPrior to 2015, white‐tailed deer (Odocoileus virginianus) were allowed to be raised and sold in‐state and out‐of‐state, but not hunted behind high fences in North Carolina, USA. As a result of the proliferation of Chronic Wasting Disease (CWD) in other states, the North Carolina Wildlife Resources Commission (WRC) delayed a decision, requested by the state's deer farmers, to approve increasing the size of current deer farms, permitting new deer farms, or allowing importation of deer from other states. This led to a conflict among the deer farmer, hunting and conservation groups, the WRC, and the state legislature. Despite the appointment of a WRC task force to study the potential impact of such rules on the proliferation of CWD and substantial public and legislative opposition to such changes, the legislature approved the changes in the last hours of its 2015 session. In addition, regulatory authority over deer farms was transferred from the WRC to the North Carolina Department of Agriculture and Consumer Services. The history of this recent conflict, the implications of these changes, and lessons learned should be of interest to all wildlifers. © 2016 The Wildlife Society.
Exploring perceptions about chronic wasting disease risks among wildlife and agriculture professionals and stakeholders
Authors: Schuler Krysten L. , Wetterau Alyssa M. , Bunting Elizabeth M. , Mohammed Hussni O. 
Published: March 2016

ABSTRACTChronic wasting disease (CWD) is a fatal disease of North American Cervidae. New York State (NYS, USA) successfully managed an outbreak of CWD in 2005 in both captive and wild white‐tailed deer (Odocoileus virginianus) with no reoccurrence of the disease as of 2015. To attain maximum compliance and efficacy of management actions for prevention of CWD entry, understanding the varied risk perceptions will allow for targeted, proactive communication efforts to address divergences between expert‐derived risk assessments and stakeholder risk perceptions. We examined perceived risks associated with CWD introduction and exposure among agricultural and wildlife agency professionals within and outside of NYS, as well as stakeholder groups (e.g., hunters and captive cervid owners). We measured perceived risk using a risk assessment questionnaire online via Qualtrics survey software and evaluated similarities within, as well as differences in, perception among participant groups. New York State biologists employed by the Department of Environmental Conservation and independent non‐NYS wildlife and agricultural professionals thought CWD risks associated with captive cervids were high; captive cervid owners thought risks for wild and captive cervids were low. Agriculture and wildlife professional groups agreed on general risk perceptions. We ranked 15 individual risk hazards into high and low–medium categories based on all responses. Differences between groups were most evident in hypothetical disease pathways. Any pathway involving inter‐state import of live cervids received high ranking for all groups except captive cervid owners. Comparatively low‐risk perceptions by captive cervid operators may stem from misinformation, lack of understanding of testing programs, and indemnity payments for animal depopulation. Communication and education directed at areas of disagreement may facilitate effective disease prevention and management. © 2016 The Wildlife Society.
Captive white‐tailed deer industry—Current status and growing threat
Authors: Adams Kip P. , Murphy Brian P. , Ross Matthew D. 
Published: March 2016

ABSTRACTIn 2012, 10 states in the United States debated legislation to introduce or expand captive white‐tailed deer (Odocoileus virginianus) breeding operations. Because this was far more than any prior year, we surveyed 37 state wildlife agencies in the eastern contiguous United States to determine the scope and intensity of captive operations and state‐specific requirements. Thirty‐seven states provided some data. Twenty of 32 states reported 5–1,332 breeding facilities holding >140,000 white‐tailed deer; 20 of 29 states reported 1–150 shooting preserves holding >25,000 white‐tailed deer. Captive white‐tailed deer were classified as wildlife in 12 of 22 states (55%), livestock in 8 states (36%), and game animals in 2 states (9%). Only 5 of 21 states (24%) had minimum acreage requirements for breeding facilities, but 13 of 20 states (65%) did for shooting preserves. Four of 16 states (25%) had a minimum release time before deer could be shot in a preserve. Six of 21 states (29%) had stocking density requirements, with 6 of 25 states (24%) listing habitat requirements for captive white‐tailed deer facilities. Seventeen of 23 states (74%) required external tagging, and 24 of 27 states (89%) allowed consumption of white‐tailed deer killed in shooting preserves. We believe the captive white‐tailed deer industry undermines the North American Model of Wildlife Conservation, threatens the health of wild deer, and undermines the public's perception of hunting. A better understanding of this growing industry will help managers safeguard free‐ranging white‐tailed deer populations and the future of deer hunting. © 2016 The Wildlife Society.
Diseases associated with translocation of captive cervids in North America
Authors: Gerhold Richard , Hickling Graham 
Published: March 2016

ABSTRACTThe privatization of captive cervids, with associated interstate movement of cervids, poses a substantial health risk to native free‐ranging wildlife and domestic animals in North America. Captive cervid operations provide an avenue for transmission of diseases such as chronic wasting disease that could have significant impact on wild cervid populations. In addition, other infectious parasites and pathogens that are potentially associated with captive cervid operations and translocation include, but are not limited to the agents of brucellosis (Brucella abortus), bovine tuberculosis (Mycobacterium bovis), hemorrhagic disease (Orbivirus spp.), bovine viral diarrhea (Pestivirus spp.), deer meningeal worm (Parelaphostrongylus tenuis), Johne's disease (Mycobacterium avium paratuberculosis), and various arthropod‐borne diseases. Transmission of disease agents into previously uninfected states and provinces through translocation of live animals can have adverse consequences. Monitoring and treatment to combat disease outbreaks associated with livestock–wildlife transmission costs states and provinces hundreds of millions of dollars. Inconsistency in jurisdiction, financial responsibility, and indemnity can lead to distrust and poor working relationships between state or provincial wildlife and agriculture departments. Although infectious disease transmission to humans has not been historically associated with wild deer, the translocation of cervids in conjunction with captivity can potentially lead to transmission of zoonotic disease agents including brucellosis and tuberculosis. We summarize the various disease agents associated with captive cervid operations and activities, potential for disease transmission to native wildlife and livestock, and associated costs. Proactive, rather than reactive, actions to prevent disease transmission from captive to wild animals should be of high priority for animal managers and regulatory agencies. © 2016 The Wildlife Society.
Effects of Minimally Invasive Esophagectomy and Open Esophagectomy on Circulating Tumor Cell Level in Elderly Patients with Esophageal Cancer
Authors: Wang Hai‐Bo , Guo Qiang , Li Yong‐Hui , Sun Zhen‐Qing , Li Ting‐Ting , Zhang Wen‐Xue , Xiang Sha‐Sha , Li He‐Fei 
Published: March 2016

AbstractObjective
To investigate the effects of minimally invasive esophagectomy (MIE) and open esophagectomy (OE) on circulating tumor cell (CTC) level of elderly patients with esophageal cancer (EC).MethodsA total of 78 elderly EC patients who aged over 64 years were divided into the MIE group (n = 40) and the OE group (n = 38). CTC enrichment was performed through CD326 (EpCAM) immunomagnetic beads positive sorting, and then labeled by CK‐PE and CD45. The quantity of CTCs was measured by multiparameter flow cytometry. Double antibody sandwich enzyme‐linked immuno sorbent assay ELISA (DAS‐ELISA) was used for detecting the levels of IL‐6, IL‐10, and IFN‐γ.ResultsAmong the 78 elderly EC patients, CTC level after the surgery was higher than that during the surgery, and CTC level during surgery was higher than that before the surgery (both P < 0.05). Postoperative CTC level in the MIE group was lower than that in the OE group, and the variation of CTC level from pre‐operation to intra‐operation in the MIE group was also lower than that in the OE group (both P < 0.05). Furthermore, there was significant difference in the incidences of intra‐operative and postoperative complications between the MIE group and the OE group (17 cases vs. 31 cases, P < 0.05), and the CTC levels of the patients with complications in either group were significantly higher than the patients without complications (both P < 0.05). IL‐6 and IL‐10 levels significantly increased, while IFN‐γ level decreased in both groups during the surgery and 3 days after the surgery compared to those before the surgery; 2 weeks after the surgery, IL‐6 and IL‐10 levels in the MIE group recovered to the pre‐operative levels (all P < 0.05). However, in the OE group, IL‐6 and IL‐10 levels 2 weeks after the surgery were still significantly higher than those before the surgery (all P < 0.05); IFN‐γ levels in both groups recovered to the pre‐operative levels, with higher level in the MIE group than that in the OE group (P < 0.05).ConclusionMIE helped to reduce the survival rate of tumor cells in peripheral blood at the early period of postoperation, and dynamic monitoring CTC level could be used to evaluate the prognosis of EC patients.
Proteomic analysis to elucidate degeneration of Clostridium beijerinckii NCIMB 8052 and role of Ca2+ in strain recovery from degeneration
Authors: Lv Jia , Jiao Shengyin , Du Renjia , Zhang Ruijuan , Zhang Yan , Han Bei 
Published: March 2016

Abstract
Degeneration of solventogenic Clostridium strains is one of the major barriers in bio-butanol production. A degenerated Clostridium beijerinckii NCIMB 8052 strain (DG-8052) was obtained without any genetic manipulation. Supplementation of CaCO3 to fermentation medium could partially recover metabolism of DG-8052 by more than 50 % increase of cell growth and solvent production. This study investigated the protein expression profile of DG-8052 and its response to CaCO3 treatment. Compared with WT-8052, the lower expressed proteins were responsible for disruption of RNA secondary structures and DNA repair, sporulation, signal transduction, transcription regulation, and membrane transport in DG-8052. Interestingly, accompanied with the decreased glucose utilization and lower solvent production, there was a decreased level of sigma-54 modulation protein which may indicate that the level of sigma-54 activity may be associated with the observed strain degeneration. For the addition of CaCO3, proteomic and biochemical study results revealed that besides buffer capacity, Ca2+ could stabilize heat shock proteins, increase DNA synthesis and replication, and enhance expression of solventogenic enzymes in DG-8052, which has a similar contribution in WT-8052.
Effect of salpingectomy, ovarian cystectomy and unilateral salpingo-oopherectomy on ovarian reserve
Authors: Rustamov Oybek , Krishnan Monica , Roberts Stephen A , Fitzgerald Cheryl T 
Published: March 2016

AbstractPelvic surgery can affect ovarian reserve, but estimates of the potential effect of different surgical procedures are lacking. This study examines the markers of ovarian reserve after different procedures in order to help the provision of informed consent before surgery. Anti-Müllerian hormone (AMH), antral follicle count (AFC) and follicle-stimulating hormone (FSH) of women with a history of salpingectomy, ovarian cystectomy or unilateral salpingo-oophorectomy were compared to those without history of surgery using cross-sectional data adjusting for patient and clinical factors in multivariable regression model. There were 138 women who had had salpingectomy, 36 unilateral salpingo-oopherectomy, 41 cystectomy for ovarian cysts that are other than endometrioma and 40 women had had excision of endometrioma. There was no significant difference in AMH (9 %; p = 0.33), AFC (−2 %; p = 0.59) or FSH (−14 %; p = 0.21) in women with a history of salpingectomy compared to women without surgery. Women with a history of unilateral salpingo-oophorectomy were found to have significantly lower AMH (−54 %; p = 0.001). These women also had lower AFC (−28 %; p = 0.34) and higher FSH (14 %; p = 0.06), the effect of which did not reach statistical significance. The study did not find any significant associations between a history of cystectomy, for disease other than endometrioma and AMH (7 %; p = 0.62), AFC (13 %; p = 0.18) or FSH. (11 %; p = 0.16). Women with a history of cystectomy for ovarian endometrioma had 66 % lower AMH (p = 0.002). Surgery for endometrioma did not significantly affect AFC (14 %; p = 0.22) or FSH (10 %; p = 0.28). Salpingo-oopherectomy and cystectomy for endometrioma cause a significant reduction in AMH levels. Neither salpingectomy nor cystectomy for cysts other than endometrioma has appreciable effects on ovarian reserve.
Cardiac dynamics: Alternans and arrhythmogenesis
Authors: Tse Gary , Wong Sheung Ting , Tse Vivian , Lee Yee Ting , Lin Hiu Yu , Yeo Jie Ming 
Published: March 2016

AbstractPre‐existing heterogeneities present in cardiac tissue are essential for maintaining the normal electrical and mechanical functions of the heart. Exacerbation of such heterogeneities or the emergence of dynamic factors can produce repolarization alternans, which are beat‐to‐beat alternations in the action potential time course. Traditionally, this was explained by restitution, but additional factors, such as cardiac memory, calcium handling dynamics, refractory period restitution, and mechano‐electric feedback, are increasingly recognized as the underlying causes. The aim of this article is to review the mechanisms that generate cardiac repolarization alternans and convert spatially concordant alternans to the more arrhythmogenic spatially discordant alternans. This is followed by a discussion on how alternans generate arrhythmias in a number of clinical scenarios, and concluded by an outline of future therapeutic targets for anti‐arrhythmic therapy.
Examining pathways to safety and financial performance in hospitals: A study of lean in professional service operations
Authors: Dobrzykowski David D. , McFadden Kathleen L. , Vonderembse Mark A. 
Published: March 2016

AbstractHealthcare operates in a complex professional service environment that faces challenges in delivering high quality and affordable care – a set of goals that some healthcare professionals believe are incongruous. To consider opportunities to achieve these important outcomes, this study examines the relationships among comprehensive lean orientation, internal integration, patient safety, and financial performance. This study draws on the professional services operations (PSOs) literature stream to explain and offer a means of addressing the complexity of healthcare delivery. Grounded in dynamic capabilities, this study develops a model for improving financial performance and patient safety in hospitals. Using structural equation modeling, the model is tested with survey data from 211 acute care hospitals in the USA that are matched with data from two other sources – objective patient safety data from the Centers for Medicare and Medicaid Services and objective net income data from the American Hospital Directory. Results indicate that although a comprehensive lean orientation has a direct and positive impact on patient safety, it impacts financial performance indirectly through internal integration. The results have major implications for enhancing patient safety and financial performance in healthcare service organizations, and improving operations in PSOs more broadly.
The impact of computerized provider order entry on emergency department flow
Authors: Gray Andrew , Fernandes Christopher M.B. , Van Aarsen Kristine , Columbus Melanie 
Published: March 2016

AbstractObjectivesComputerized provider order entry (CPOE) has been established as a method to improve patient safety by avoiding medication errors; however, its effect on emergency department (ED) flow remains undefined. We examined the impact of CPOE implementation on three measures of ED throughput: wait time (WT), length of stay (LOS), and the proportion of patients that left without being seen (LWBS).MethodsWe conducted a retrospective cohort study of all ED patients of 18 years and older presenting to London Health Sciences Centre during July and August 2013 and 2014, before and after implementation of a CPOE system. The three primary variables were compared between time periods. Subgroup analyses were also conducted within each Canadian Triage and Acuity Scale (CTAS) level (1–5) individually, as well as for admitted patients only.ResultsA significant increase in WT of 5 minutes (p=0.036) and LOS of 10 minutes (p=0.001), and an increase in LWBS from 7.2% to 8.1% (p=0.002) was seen after CPOE implementation. Admitted patients’ LOS increased by 63 minutes (p<0.001), the WT of CTAS 3 and 5 patients increased by 6 minutes (p=0.001) and 39 minutes (p=0.005), and LWBS proportion increased significantly for CTAS 3–5 patients, from 24.3% to 42.0% (p<0.001) for CTAS 5 patients specifically.ConclusionsCPOE implementation detrimentally impacted all patient flow throughput measures that we examined. The most striking clinically relevant result was the increase in LOS of 63 minutes for admitted patients. This raises the question as to whether the potential detrimental effects to patient safety of CPOE implementation outweigh its benefits.