bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the outcomes of full thickness Skin graft in the reconstruction of concealed penis in children aged 7 to 14 years
Authors: Hosseinpour Mehrdad , Fazeli Ali , Moznebi Davood , Seyedyousefi Sarah 
Published: September 2024

Abstract

Introduction: Concealed penis is a congenital anomaly that affects not only the appearance but also the function of the external genitalia in the male sex. Different surgical methods have been proposed to correct this disorder, including removal of the previous scar, penile shaft, penile trunk skin reconstruction with flap, penile skin fixation in penopubic and penoscrotal angles, and removal of extra pubic fat. In this study, we will discuss the results of definitive surgery in one stage using autogenous skin grafts and examine the details of this technique.
Methods: This study was conducted as a clinical trial in Hospitals related to the Isfahan University of Medical Sciences. Children over the age of 7 years old. A full-thickness graft with a length of 1.5 times the defect and a width of 1 cm was taken from the non-dominant thigh and defatted. The inguinal skin was chosen because it is hairless and next to the penis, and the resulting scar is easily hidden under clothing. Then, the graft was transferred to the operation site and fixed.
Results and conclusion: We proposed a method of treatment for concealed penis, using a full-thickness inguinal graft. Our technique showed promising results with minimal and negligible complications. To fully highlight each process's benefits and limitations and evaluate them against one another, these procedures should, however, be tested on larger populations and compared comprehensively.
Trial Registration: The study was conducted in accordance with the ethical standards of the Isfahan University of Medical Sciences Research Ethics Committee (ethical code: IR.MUI.MED.REC.1402.073) And was registered on 27/05/2023 as a clinical trial in Iranian registry of clinical trials. (IRCT code: IRCT20230513058160N1)
Electroacupuncture for insomnia in Parkinson's Disease: Study protocol for a Multicenter, Randomized Clinical Trial
Authors: Gu Sichun , Li Qiang , Peng Jing , Hao Yong , Ye Qing 
Published: September 2024

Abstract

OBJECTIVE: Insomnia, a prevalent and challenging non-motor symptom of Parkinson's disease (PD), lacks specific acupuncture efficacy data within the PD patient population. The current study is designed to evaluate the effectiveness and safety of electroacupuncture (EA) in comparison to sham electroacupuncture (SA) for PD-related insomnia treatment.
DESIGN, SETTING, AND PARTICIPANTS: We will conduct a multicenter, participant-and assessor-blinded, randomized, sham-controlled clinical trial across seven neurology sites in China. Participants diagnosed with PD and experiencing insomnia will be randomly assigned to receive either EA or SA in a 1:1 ratio.
INTERVENTIONS: The trial intervention will consist of an 8-week treatment phase for EA or SA, complemented by a subsequent 12-week follow-up period.
MAIN OUTCOMES AND MEASURES: The primary outcome will be the change in the Parkinson's Disease Sleep Scale-2 (PDSS-2) score at the end of the intervention (week 8). A range of secondary subjective sleep assessments will also be utilized, including the Insomnia Severity Index (ISI), Epworth Sleepiness Scale (ESS), and the rapid eye movement sleep behavior disorder (RBD) screening questionnaire (RBDSQ). Additionally, scores from the Movement Disorder Society Unified Parkinson’s Rating Scale parts II and III (MDS-UPDRS II and III), and the Parkinson's disease questionnaire (PDQ-39) will be evaluated. For secondary objective sleep parameters, polysomnography (PSG) will provide data on total sleep time (TST), sleep efficiency (SE), wake after sleep onset (WASO), sleep onset latency, REM latency, the proportion of each sleep stage (N1, N2, N3, and REM), the apnea-hypopnea index (AHI), and mean sleep latency (MSL).
TRIAL REGISTER: This trial was registered with the Chinese Clinical Trial Registry on February 26, 2024, https://www.chictr.org.cn/, ChiCTR2400081162.
Upregulated ATG4B Predicts Poor Prognosis and Correlates with Angiogenesis in Osteosarcoma
Authors: Khalil Elzahraa Ibrahim Mohamed , Mohamed Fatma El Zahraa Ammar , Kamal Rehab Mohamed 
Published: September 2024

Abstract

Background: Osteosarcoma (OS) is the most common primary bone cancer in children and adolescents. Patients with metastatic OS experience significantly poorer outcomes, largely due to resistance to chemotherapy. Between 35-45% of these patients do not respond to standard chemotherapeutic treatments, resulting in a very low 5-year survival rate of only 5-20%. This resistance often leads to treatment failures and unfavorable prognoses, highlighting the critical need for new therapeutic targets to improve treatment strategies. Autophagy-related gene 4 B (ATG4B) is a crucial cysteine protease for autophagosome formation. It is overexpressed and correlates with poor prognosis in various cancers. However, the relationship between ATG4B expression and angiogenesis in osteosarcoma remains unexplored. This study investigates the expression levels of ATG4B and VEGF in osteosarcoma and their correlation with clinicopathological data.
Materials and Methods: The study included 67 paraffin-embedded osteosarcoma tissue samples. ATG4B and VEGF expression levels were assessed via immunohistochemistry, and their associations with clinicopathological variables were statistically analyzed. Additionally, ATG4B gene expression in osteosarcoma was examined using GEO data sets from https://www.ncbi.nlm.nih.gov.
Results:The analysis showed that ATG4B and VEGF were expressed in 79.1% and 74.6% of the osteosarcoma samples, respectively. There was a significant positive correlation between ATG4B expression and tumor size, tumor stage, and histological response to neoadjuvant chemotherapy, with p-values of 0.013, 0.008, and 0.022, respectively. VEGF expression also significantly correlated with tumor size, tumor stage, and the presence of distant metastasis at diagnosis, with p-values of 0.022, 0.044, and 0.013, respectively. A notable positive correlation between ATG4B and VEGF expression levels was observed (p=0.002), supported by the GEO dataset analysis.
Conclusions: The results suggest that ATG4B acts as a tumor promoter in osteosarcoma, indicating its potential as a therapeutic target to inhibit tumor growth. Elevated ATG4B levels may also serve as a marker for poor prognosis. Additionally, VEGF overexpression is linked to a higher likelihood of pulmonary metastasis and worse overall prognosis. The positive correlation between ATG4B and VEGF suggests that the absence of both markers could be indicative of a better chemotherapy response, offering insights into potential new treatment approaches.
Comparison of the inhibitory effects between bepotastine and diphenhydramine on rituximab-induced infusion-related reactions: A single-center retrospective study
Authors: Hori Tomoki , Yamamoto Kazuhiro , Nakagawa Tomoaki , Nakagawa Rinako , Okayama Masami , Sudou Tamika , Hamasaki Moe , Yasuda Mai , Kobayashi Shinya , Nakamura Fumihiko , Yagi Hideo , Kitahiro Yumi , Ikushima Shigeki , Yano Ikuko 
Published: September 2024

Abstract

Purpose
Infusion-related reaction (IRR) is a common adverse event induced by rituximab. Although first-generation histamine 1 receptor antagonists (H1RAs) are commonly used for the prevention of IRR, evidence is scarce on the incidence of IRR suppression with the second-generation H1RA bepotastine. This study assessed inhibitory effects of bepotastine on rituximab-induced IRR and compared them with those of first-generation H1RA diphenhydramine.
Methods
We retrospectively evaluated IRR incidence in patients with B-cell non-Hodgkin’s lymphoma who received their first dose of rituximab.
Results
The incidence of IRR was 9.8% in the bepotastine group (n = 92), which was significantly lower than the 30.2% incidence rate in the diphenhydramine group (n = 96; p < 0.001). The median IRR onset time after rituximab administration in the bepotastine group was 60 min, which was significantly shorter than the 120 min of the diphenhydramine group (p = 0.002). Multivariable logistic regression analysis revealed that the risk of IRR incidence was higher in patients with B symptoms (odds ratio [OR] = 2.96, 95% confidence interval [CI]: 1.20–7.27) and bulky disease (OR = 9.12, 95% CI: 2.53–32.9). Bepotastine use as premedication was an independent factor reducing the risk of IRR incidence (OR = 0.19, 95% CI: 0.08–0.47).
Conclusion
Bepotastine more effectively reduced the incidence of rituximab-induced IRR than diphenhydramine, particularly delayed-type reactions.
Comparative studies on CO2 adsorption performance of different morphologies of Silicalite-1 zeolites synthesized from fly ash
Authors: Wu Xianglian , Nulahong Aisha , Tuo Changmin , Li Jian , Xu Fei , Ren Tiezhen , Abulizi Abulikemu 
Published: September 2024

Abstract

Solid waste fly ash has a great impact on the environment and pollutes water, air and soil to different degrees. Therefore, it is of great significance to realize the high-value utilization of fly ash. In this paper, high purity SiO2 was successfully extracted from fly ash by high temperature calcination, alkali fusion activation and pickling, and then different morphologies of Silicalite-1 zeolites were synthesized by using SiO2 extracted from fly ash as silicon source, TPAOH as templating agent and NaOH as alkali source. The influencing factors such as crystallization time, crystallization temperature, NaOH dosage, TPAOH dosage and different hydrosilica ratios were investigated separately. The results show that under the conditions of crystallization time of 12 h, crystallization temperature of 180 ℃ and molar ratio of water-silicon of 42, the prepared P-S (plate-like Silicalite-1) has good morphology, high relative crystallinity and adsorption capacity of CO2 of 1.89 mmol/g. The CO2 adsorption capacity of the S-S (spherical-like Silicalite-1) prepared by adjusting the amount of TPAOH is 1.34 mmol/g. The CO2 adsorption capacity of the prepared C-S (cross-type Na-Silicalite-1) is only 1.06 mmol/g. The CO2 adsorption capacities follow P-S > S-S > C-S. The results may provide a valuable reference for zeolite-based adsorbents in the adsorption removal or recovery of CO2.
The number of available sample observations modulates gambler’s fallacy in betting behaviors
Authors: Li Yutong , Wang Di 
Published: September 2024

Abstract

The gambler’s fallacy is a commonly observed cognitive bias in betting behaviors. It refers to the erroneous belief that an independent and identically distributed random process exhibits negative serial correlation. This belief is often triggered when individuals observe a sample of historical outcomes of the random process. Our study examines the impact of providing more historical information or a larger sample of recent outcomes on the propensity for individuals to commit the gambler’s fallacy in repeated betting scenarios with varying probabilities of winning. Experimentally, we find strong evidence of both the gambler’s fallacy and the hot-outcome fallacy, which predicts the opposite pattern. These fallacies correlate respectively with the frequency and length of consecutive occurrences in the observed sample. Providing a larger sample of observations increases the tendency for the gambler’s fallacy when the probability of winning is low but significantly reduces the fallacy when probabilities are moderate or high. Our results indicate that gambling fallacies are highly sensitive to contextual factors in decision-making, and that in real-life gambling situations, which often involve low chances of winning, providing more sample observations could amplify the gambler’s fallacy.
Effect of Ionizing Radiation Exposure on NOX4 Expression in-Vitro and in-Vivo Studies: A Systematic Review and Meta-analysis
Authors: Hajimirzaei Pooya , Paydar Reza , Razmgir Maryam , Rajabinasab Fatemeh , AhmadiTabatabaei Faeze , Hamblin Michael R , Janzadeh Atousa , Taherkhani Soroush 
Published: September 2024

Abstract

Introduction: Patients exposed to ionizing radiation (IR) from various sources experience several side effects. Understanding the mechanisms through which IR induces these effects could minimize their consequences. This study investigates the activation pathway of NADPH oxidase 4 (NOX4) after exposure to IR.
Methods: The analysis incorporated studies that assessed NOX4 expression as an outcome variable. The study involved searches across various databases. A total of 58 articles were included in the meta-analysis, and data extracted from these studies were analyzed using Comprehensive Meta-Analysis Software.
Results: Analysis of the impact of IR on NOX4 expression, demonstrated a notable increase in protein expression in animals (SMD=3.452; p<0.001), in normal cells in vitro (SMD=2.689; p<0.001), and in cancer cells (SMD=2.159; p<0.05). Furthermore, there was a significant increase in NOX4 mRNA expression in animals (SMD=5.070; p<0.001), in normal cells in vitro (SMD=3.563; p<0.001), and in cancer cells (SMD=3.280; p<0.001). Subgroup analysis was conducted based on the tests utilized to measure NOX4 expression, various organs, IR parameters, and follow-up time after IR.
Conclusion: NOX4 plays a crucial role in mediating radiation-induced damage in many organs. The upregulation of NOX4 expression in these organs is influenced by factors such as the radiation dose and source. Additionally, there was a further increase in NOX4 protein expression over time, highlighting its potential role in the progression of radiation-induced damage in vital organs.
Trends of impairment of the activities of daily living in Chinese elderly: An age-period-cohort analysis
Authors: Zhang Xianqi , Li Xiaoju , Zhao Li , Fan Rong , Dong Jiaxin , Shen Xiaoying , Li Yiyao 
Published: September 2024

Abstract

Background
Based on age, period, and cohort, this study aimed to understand the trend of impairment of activities of daily living (ADL) and its influencing factors in older adults in China and to provide a basis for the development of appropriate interventions.
Methods
This longitudinal follow-up study was conducted on a sample of 40748 cases aged ≥ 60 years from the China Family Panel Studies (CFPS) database of 6 periods, using the six-item ADL scale to measure impaired ability to perform daily activities in older adults. Incorporating independent variables affecting ADL impairment in older adults based on the health ecology theory, a stratified age-period-cohort mixed-effects model was used to analyze the factors influencing impaired ADL in older adults.
Results
Age, different periods, and birth cohort had an independent effect on females suffering from chronic diseases, having lower life satisfaction (dissatisfied, very dissatisfied), living in the western region, residing in rural areas, having per capita household income levels of middle-high, middle-low, lower-middle, lower-middle, and going to specialized and general hospitals were risk factors for ADL impairment in older adults. Smoking, drinking alcohol, exercising, having high life satisfaction (satisfied), being married, having higher education, and having urban/rural/rural residents’ health insurance and employee health insurance were protective factors for ADL impairment in older adults.
Conclusions
It is important to pay further attention to the current situation of ADL impairment in older adults from the perspective of the whole life cycle and the whole population and to take timely and targeted intervention strategies and preventive measures to improve the health of older adults in different dimensions from the individual to the macro level.
A Comprehensive Seroepidemiology of Dengue and Chikungunya Arboviruses in Iran, 2020-2023
Authors: Seyed-Khorami Seyed-Mahmood , Mostafavi Ehsan , Gerdooei Sepideh , Sajadi Seyed Marzieh , Asadi Laya Farhan , Jalali Tahmineh , Pouriayevali Mohammad Hassan , Nikpour Fatemeh , Mirolyaei Abdolreza , Raeisi Ahmad , Arshi Shahnam , Gouya Mohammad Mehdi , Enayati Ahmadali , Zaim Morteza , Salehi-Vaziri Mostafa 
Published: September 2024

Abstract

Aim: are the most common arthropod-borne viruses (Arboviruses) globally. So far, no autochthonous cases of DENV and CHIKV infections have been reported in Iran. Nevertheless, due to the recent identification of Aedes aegypti in the south and Aedes albopictusin the north of Iran, the country is at a serious risk of local outbreaks of these infections.
Methods: Between 2020 and 2023, a comprehensive cross-sectional study was conducted to explore the anti-DENV and CHIKV IgG antibodies in the general populations of both southern and northern regions of Iran using ELISA.
Results: A total of 11192 participants were included, of whom 2.3% (256 individuals) and 0.1% (11 cases) were DENV seropositive and CHIK seropositive, respectively. Across the eight examined provinces, Sistan and Baluchestan province displayed the highest prevalence of dengue seropositive cases (6.80%) (P<0.001). Samples collected in 2022 demonstrated a notably higher seropositive rate of 5.5% (P<0.001). Interestingly, all 11 CHIKV seropositive cases were from Sistan and Baluchestan province.
Conclusion: The seroprevalence of DENV was notably higher in southern regions, particularly in Sistan and Baluchestan province and CHIKV seropositive cases were exclusively found in Sistan and Baluchestan province. This study furnishes crucial insights into the seroepidemiology of DENV and CHIKV in Iran which, in conjunction with the presence of Ae. Aegypti in the south and Ae. Albopictus in the north of Iran, emphasizes the requirement of improving integrated surveillance and control systems in the whole of the country, especially in the south of Iran.
Detection of soft-tissue interposition in Watson-Jones type IV proximal tibial epiphysis injury by FRACTURE: A case report
Authors: Omichi Yasuyuki , Sugimine Yuto , Momota Kaori , Takai Michihiro , Sato Ryosuke , Enishi Tetsuya , Goto Tomohiro , Nakano Shunji , Nishiyama Yukako , Sairyo Koichi 
Published: September 2024

Abstract

Fast field echo resembling a CT using restricted echo-spacing (FRACTURE) is a method of magnetic resonance bone imaging that provides CT-like image contrast based on a high-resolution 3D gradient sequence. This is the first report to describe the detection of soft-tissue interposition in a patient with proximal tibial epiphysis injury by FRACTURE. We present a case of Watson-Jones type IV proximal tibial epiphysis injury in an 11-year-old boy. FRACTURE revealed soft-tissue interposition into the epiphyseal line. Intraoperatively, soft-tissue interposition to the epiphyseal line was confirmed. In addition to the benefit of radiation-free imaging, FRACTURE also allows for the detailed assessment of fracture and the prediction of soft-tissue interposition, which may be useful in determining the treatment strategy for proximal tibial epiphysis injury.
Integrating Functional Proteomics and Next Generation Sequencing Reveals Potential Therapeutic Targets for Taiwanese Breast Cancer
Authors: Ku Wei-Chi , Liu Chih-Yi , Huang Chi-Jung , Liao Chen-Chung , Huang Yen-Chun , Kong Po-Hsin , Chen-Chan Hsieh , Tseng Ling-Ming , Huang Chi-Cheng 
Published: September 2024

Abstract

Integrating functional proteomics and next-generation sequencing (NGS) offers a comprehensive approach to unraveling the molecular intricacies of breast cancer. This study investigates the functional interplay between genomic alterations and protein expression in Taiwanese breast cancer patients. By analyzing 61 breast cancer samples using tandem mass tag (TMT) labeling and mass spectrometry, coupled with whole-exome sequencing (WES) or targeted sequencing, we identified key genetic mutations and their impact on protein expression. Notably, pathogenic variants in BRCA1, BRCA2, PTEN, and PIK3CA were found to be clinically relevant, potentially guiding targeted therapy decisions. Additionally, we discovered trans correlations between specific gene alterations (FANCA, HRAS, PIK3CA, MAP2K1, JAK2) and the expression of 22 proteins, suggesting potential molecular mechanisms underlying breast cancer development and progression. These findings highlight the power of integrating proteomics and NGS to identify potential therapeutic targets and enhance personalized medicine strategies for Taiwanese breast cancer patients.
Prognostic impact of psychoeducation program completion on inpatients with schizophrenia: a pilot cohort study
Authors: Noguchi Hiroki , Tarutani Seiichiro , Takei Yoshiki , Matsumoto Koichi , Okamura Takehiko , Yoneda Hiroshi 
Published: September 2024

Abstract

Background
Psychoeducation programs can reduce the risk of recurrence and readmission in patients with schizophrenia. However, almost all previous studies of program efficacy have included only patients completing the program, which may not be possible in all cases. The objective of this pilot cohort study was to compare the prognoses of inpatients with schizophrenia who did or did not complete a well-established institutional psychoeducation program.
Methods
This study is a pilot cohort study, and the participants were 32 inpatients in the psychiatric acute care ward. Among these patients, 18 completed the institutional psychoeducation program by discharge, whereas 14 missed one or more sessions for various reasons. The primary outcome was the duration of outpatient treatment (DOT) during the 5-year follow-up period, and the secondary outcomes were comparisons of the risk of all-cause discontinuation for outpatient treatment and correlations between the program participation rate and DOT.
Results
DOT was significantly longer in the program completion group than in the noncompletion group (918.2 (174.3) days vs. 225.5 (35.7) days, p = 0.001), and Cox proportional hazards regression analysis revealed that program noncompliance was associated with a 4.089-fold (p = 0.002) greater risk of discontinuation of outpatient treatment according to univariate analysis and a 2.937-fold (p = 0.030) greater risk according to multivariate analysis. A significant weak correlation was found for DOT and rates of sessions admitted to the programme (Pearson's r = 0.384, p = 0.030).
Conclusions
Completion of a psychoeducation program dramatically enhanced the success of outpatient treatment. Because of the significant impact of outpatient treatment on prognosis, inpatient psychoeducation programs should be sufficiently flexible to provide opportunities for completion.
Single-cell transcriptomic analysis reveals impaired mitochondrial gene expression in the podocytes of a child with primary coenzyme Q10 nephropathy.
Authors: Park Peong Gang , Choi Sowon , Ahn Yo Han , Kim Seong Heon , Kim Chaeyoon , Kim Hyun Je , Kang Hee Gyung 
Published: September 2024

Abstract

Background
Coenzyme Q10 (CoQ10) nephropathy is a well-known cause of hereditary steroid-resistant nephrotic syndrome, primarily impacting podocytes. This study aimed to elucidate variations in individual cell-level gene expression in CoQ10 nephropathy using single-cell transcriptomics.
Methods
We conducted single-cell sequencing of a kidney biopsy specimen from a 5-year-old boy diagnosed with a CoQ10 nephropathy caused by a compound heterozygous COQ2 mutation. The analysis focused on the proportion of cell types, differentially expressed genes in each cell type, changes in gene expression related to mitochondrial function and oxidative phosphorylation (OXPHOS).
Results
Our findings revealed a uniform downregulation of mitochondrial gene expression across various cell types in the context of these mutations. Notably, there was a specific decrease in mitochondrial gene expression across all cell types. The study also highlighted an altered immune cell population proportion attributed to the COQ2 gene mutation. Pathway analysis indicated a downregulation in OXPHOS and an upregulation of various synthesis pathways, particularly in podocytes.
Conclusion
This study improves our understanding of CoQ10 nephropathy's pathogenesis and highlights the potential applications of single-cell sequencing in hereditary kidney diseases.