bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dioscin protects against chronic prostatitis through the TLR4/NF-κB pathway
Authors: Long Yan , Ge Xiaodong , Ma Liangliang , Guo Junhua , Zhu Yong 
Published: September 2024

Abstract
This study aimed to elucidate the effects and potential mechanisms of dioscin on chronic prostatitis (CP) in vivo and in vitro. CP models were constructed in vivo and in vitro and treated with different concentrations of dioscin. Hematoxylin and eosin staining was used to investigate the morphology of the prostate tissues. The concentration of inflammatory factors in prostate tissues was determined by enzyme-linked immunosorbent assay. The release of reactive oxygen species, malondialdehyde, superoxide dismutase, and catalase was measured using detection kits. P69 cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Furthermore, the activity of the TLR4/NF-κB signaling pathway was determined by quantitative reverse transcriptase polymerase chain reaction or Western blot assay. Histopathological data suggested that dioscin exerted protective effects against prostate morphological changes. Dioscin inhibits inflammatory cytokines and oxidative stress (OS) in prostate tissues in a concentration-dependent manner. Moreover, dioscin notably inhibited the activation of the TLR4/NF-κB signaling pathway in CP rats. In vitro, dioscin remarkably reduced lipopolysaccharide-induced P69 proliferation, inflammation, OS, and TLR4/NF-κB pathway activation in a dose-dependent manner. In conclusion, dioscin exerts a protective effect in CP by decreasing the inflammatory response and OS through the TLR4/NF-κB pathways. Our findings provide a novel latent therapy for dioscin for the treatment and prevention of CP.
Synthesis, rheology, cytotoxicity and antibacterial studies of N-acrolylglycine-acrylamide copolymer soft nano hydrogel
Authors: Yamala Anilkumar , Kurba Jai Shree , Kumar D. Sanjeev , Kanaparthi Ravi Kumar , Madikonda Ashok K. 
Published: September 2024

Abstract
Hydrogels possess excellent biological properties that make them ideal for biomedical applications. They are compatible with living cells and tissues because they can swell in the presence of water. In this study, we investigated the stability and biocompatibility of hydrogels. We synthesized and characterized N-acryl glycine (NAG) monomer and then synthesized its copolymer using the miniemulsion-polymerization technique, a soft-hydrogel method. To confirm the morphological properties of the dried hydrogel particles, we used the field emission scanning electron microscopy (FESEM) technique. We also investigated the rheological properties of the hydrogels for different concentrations to evaluate their mechanical strength and gel-like properties. Our findings indicated that the 10 % gel had superior strength and yield strain at all examined temperatures (30 °C, 37 °C, and 40 °C) compared to other concentrated gels. We systematically evaluated the biocompatibility of the hydrogel using three different cell lines: HEK 293T, RAW 264.7, and HeLa. Our cell line studies demonstrated that hydrogels are viable when exposed to a concentration of 0.5 mg/mL. Moreover, cell proliferation was observed at concentrations below 0.25 mg/mL. The MTT assay and rheology results suggest that hydrogel characteristics are more suitable for various biomedical applications, such as drug delivery and tissue engineering.
Proteomics to Study Parchment Degradation – From Bulk to Spatial Analysis
Authors: Malissa Antonia , Cappa Federica , Schreiner Manfred , Marchetti-Deschmann Martina 
Published: September 2024

Abstract
Implementing biomolecular techniques in the study of written cultural heritage has led to a steady development of biocodicology and the study of DNA and proteins in parchment in the past years. In this context, matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) has gained increasing attention as a powerful tool to study the ancient writing support. With a particular focus on the main components of parchment – different types of collagens – proteomic applications of the technique are highlighted. The review summarises advances in biocodicological studies focusing on manufacturing aspects and conservation treatments. Herein we further investigated proteomic studies on animal skin to prove the potential of MALDI-MS imaging (MALD-MSI) to broaden our knowledge and take studies on damage assessment and degradation of parchment to another level.
The effect of addition of sodium hexadecyl sulfate on the performance properties of lauramidopropyl betaine
Authors: Chavan Akshaya Ravindra , Bhagwat Sunil S. 
Published: September 2024

Abstract
Palm fatty acid distillates can be used to produce fatty alcohol sulphates. The sodium hexadecyl sulphate (SHS) of the C16 fatty acid thus formed has a high Krafft point which limits its use in applications. Here we propose the use of C16 sulphate mixed with the amphoteric lauramidopropyl betaine (LAPB). The solubility of SHS in LAPB was determined and mixtures of the two were prepared to the extent of their solubility. The interfacial properties of the surfactant mixtures were investigated. The CMC of the surfactant mixtures was determined using dye solubilisation. The performance properties of the mixtures, such as foaming and viscosity, were analysed. It was concluded that the addition of even small amounts of SHS to LAPB causes a significant change in its CMC, although the performance properties of LAPB in terms of foaming and viscosity are maintained.
Reproductive health in trans and gender diverse patients: Transgender medicine: contextual trans gynecology
Authors: Trotsenburg Mick van 
Published: September 2024

In brief
This manuscript aims to promote an understanding of the special needs of transgender and gender-diverse people by putting trans gynecology into context. By definition, medical interventions for transgender people are not different from the treatment of cisgender people but cannot be singled out as gender-affirmative treatment. Treatment is dedicated to an overarching goal beyond medicine, namely to enable and maintain social functioning under the signs of the social gender. It is therefore that this narrative overview intensively deals with the requirements of trans gynecology. Also, various gynecological disorders are touched upon, provided they are relevant to trans people. The different significance of gynecological symptoms for either trans men or trans women, and the effects of supraphysiological androgen treatment on hormone-sensitive tissue, are stressed.

Abstract
Transgender health care is not just gender-affirmative transitional care but is committed to a superior objective, often beyond a medical perspective: to create and maintain physical conditions for social functioning under the signs of the individually appropriate sex and to contribute to significantly reducing gender dysphoria. For these purposes, it is a prerequisite to have a distinct contextual understanding of the complex reality of transpeople and knowledge about the numerous facets of transgender healthcare. Gynecology for transgender and gender-diverse people does not differ greatly from gynecology for cisgender female patients except in goals and context. Relief from complaints derived from genital organs is, of course important, but for transpeople there always is an overarching gender dimension that sometimes complicates treatment and might give rise to misunderstandings. Also, minority stress caused by societal factors frequently impacts the mental and physical state of health negatively and needs to be considered. This paper focuses on the context of trans gynecology and addresses various contentual aspects for both transmale patients having left genital organs in situ and transfemale patients with gynecological demands. Gynecological topics are addressed, highlighting their relevance for transgender and gender-diverse people (TGD), including the effects of supra-physiological androgen exposure on ovaries and uterus, vaginal bleeding, pelvic pain under testosterone treatment, screening policies, and benign gynecological disorders as clinical manifestations may appear differently and treatment may be more burdensome.
Curiously interpreted heritage, or how a Catacomb saint in Tokaj-Hegyalja became a pope?
Authors: Kis Tímea N. 
Published: September 2024

AbstractThe relics of the Catacomb Saint Urban, which are preserved in Monok, Hungary, were falsely identified as the mortal remains of Pope Urban I in 1983. Spreading of the Pope's patronage of preserving the grapes from the spring frost damage led to the development of his new local cult.The study deals with this misidentification and its consequences, with the circumstances of the baroque reliquary's arrival to the village in 1771, with its procurer and the real reason of the purchase, finally with its translatio to the Holy Cross altar in the chapel located in the Andrássy Castle.
The influence of age on gambling problems worldwide: A systematic review and meta-analysis of risk among younger, middle-aged, and older adults
Authors: Dellosa Georgia , Browne Matthew 
Published: September 2024

AbstractBackground and AimsProblem gambling (PG) represents a significant public health concern with widespread effects in various cultures and regions globally, with younger individuals and males at a particularly higher risk. This disparity is attributed to a mix of cultural, developmental, and biological influences. To date, there has not been a comprehensive examination to determine whether this risk pattern holds consistently across different jurisdictions.MethodsWe performed a systematic review and meta-analysis using the PRISMA framework, identifying 21 eligible studies from 18 countries, encompassing 156,249 participants (47.6% male and 52.4% female).ResultsThe studies varied considerably by region (Asia: 19%, Europe: 52%, Oceania: 19%, North America: 10%), the diagnostic criteria for PG, and participation rates in gambling (ranging from 12% to 92%). Data on PG prevalence was categorised by gender and three age groups (young: 18–35, middle: 30–55, and older: 45–65). Using a random-effects meta-analysis, we found a global PG prevalence of 1.9%. Europe reported a significantly lower prevalence (1.3%) compared to North America (5.3%). Men were found to be 3.4 times more likely than women to engage in problem gambling, although the gap narrows in North America. The young demographic showed a 1.51 times higher likelihood of reporting PG compared to the middle-aged group, whereas older adults were 0.80 times less likely to report PG. Notably, age-related effects varied significantly across regions.ConclusionsOur findings confirm that age and gender significantly influence PG risk across cultures, with significant heterogeneity observed across jurisdictions.
STRATEGI IMPLEMENTASI PERJANJIAN KERJA LAUT BAGI AWAK KAPAL PERIKANAN: STUDI KASUS DI PELABUHAN PERIKANAN SAMUDERA NIZAM ZACHMAN JAKARTA
Authors: Nugroho Kun Budi , Suherman Agus , Mudzakir Abdul Kohar , Hernuryadin Yayan 
Published: September 2024

Perjanjian kerja laut (PKL) dibuat sebagai perlindungan atas risiko kerja bagi awak kapal perikanan. Pelabuhan Perikanan Samudera Nizam Zachman (PPSNZ) merupakan pelabuhan perikanan terbesar di Indonesia, sehingga PKL menjadi sangat penting untuk diimplementasikan. Penelitian ini bertujuan untuk menganalisis pelaksanaan PKL di PPSNZ, merumuskan alternatif strategi, dan menentukan prioritas strategi implementasi PKL di PPSNZ. Penelitian dilaksanakan pada Desember 2022 sampai April 2023. Metode deskriptif digunakan dengan pendekatan studi kasus. Wawancara dilakukan terhadap 100 responden yang ditentukan melalui purposive sampling. Analisis SWOT dan AHP digunakan dalam menganalisis data. Implementasi kebijakan mengacu pada teori George C. Edwards III (1980). Hasil penelitian menunjukan bahwa PKL bagi awak kapal perikanan telah diimplementasikan di PPSNZ. Terdapat beberapa kendala dalam implementasi PKL di PPSNZ seperti: adanya awak kapal yang kabur, keterbatasan pemahaman awak kapal, lemahnya basis data dan kurangnya peran asosiasi awak kapal. Urutan prioritas strategi implementasi PKL di PPSNZ adalah mengoptimalkan sumber daya, menciptakan sistem berbasis elektronik dan terintegrasi, pendampingan dan pemberlakuan sanksi, serta sosialisasi.Sea work agreements (PKL) are made as protection against the work risks for fishing vessel crew. Nizam Zachman Ocean Fishing Port (PPSNZ) is the largest fishing port in Indonesia, so PKL is very important to be implemented. This research aims to analyze the implementation of PKL in PPSNZ, formulate alternative strategies, and determine strategic priorities for implementing PKL in PPSNZ. The research was carried out from December 2022 to April 2023. A descriptive method was used with a case study approach. Interviews were conducted with 100 respondents determined using purposive sampling technique. SWOT and AHP analysis were used to analyze the data. Policy implementation refers to the theory of George C. Edwards III (1980). The research results show that PKL for fishing vessel crews has been implemented at PPSNZ. There are several obstacles in the implementation of PKL in PPSNZ such as: crew members who run away, limited understanding of fishing vessel crews, weak databases, and lack of role of ship crew associations. The order of priority for the PKL implementation strategy at PPSNZ is optimizing existing resources, creating an electronic and integrated system, assistance and imposition of sanctions, and socialization.
Determination of the exploitation parameters of the Bled El Hadba phosphate deposit, Algeria
Authors: Bekhouche Youcef , Nettour Djamel , Chaib Rachid 
Published: September 2024

The beneficiation of mineral resources not only bolsters a country's economy but also improves quality of life and fosters sustainable growth. The development of the phosphate mine in the Bled El Hadba region represents a pivotal move to meet increasing demand. This study aims to develop a comprehensive 3D geographic model of the deposit, estimate its phosphate reserves, and assess the parameters and characteristics for effective exploitation. Utilizing the block model method in Surpac 6.6.2, a detailed analysis is achieved that supports informed decision-making for sustainable resource management. This approach underscores the importance of technological innovation in the strategic planning and efficient utilization of mineral resources.
The results revealed total reserves of 425,304,000 m³, equivalent to 893,138,400 tons, with an average grade of 21.65 %. The stripping ratio was determined to be 3.3:1. These findings provide valuable insights into the deposit's potential and the optimal depth range for extracting the highest concentration of P2O5. For detailed extraction planning and estimating P2O5 concentration over five-year periods from 2023 to 2066, with an average annual phosphate ore production of 20.7 million tons, Minesched software was utilized. This comprehensive approach ensures efficient resource management and maximizes the economic return from the deposit. These findings have profound implications for enhancing both the efficiency and sustainability of Algeria's mining industry. By securing a consistent supply of phosphate products, particularly for agriculture, this research addresses the rising demand for phosphates. Additionally, the data can inform strategic planning, enabling optimized resource extraction and reduced environmental impact. This contributes not only to the immediate needs of the industry but also to the long-term economic and ecological sustainability of the region. Ultimately, the study supports sustainable development by balancing industrial growth with environmental stewardship, ensuring that future generations can continue to benefit from these vital resources.
Consideration of DC/DC converter for photovoltaics with soft switching with modified push-pull current source inverter circuit
Authors: Ivakhno Volodymyr , Zamaruiev Volodymyr , Plakhtiy Oleksandr , Nerubatskyi Volodymyr , Filipieva Maryna 
Published: September 2024

The object of research is the power part of the two-stage converter. The paper evaluates the parameters of the power part of the two-stage converter with transformer isolation, designed for the transfer of solar battery energy to the 400 V constant voltage network with the possibility of maintaining the maximum power point tracker (MPPT). The primary stage of the converter is made as a push-pull current source inverter topology with an additional switch, which is installed between the common point of the transformer's primary half-windings and the common point of the primary stage switches. The primary stage switches are made as a series connection of MOSFET transistors and Schottky diodes. The secondary stage has the traditional topology of a half-bridge voltage source inverter on MOSFET. A special switching algorithm of separated commutation is described, which provides non-dissipative snubber turn-on for of the current source inverter switches and their natural zero current turn-off switching (ZCS). The role of a snubber is performed by the leakage inductance of the transformer. For voltage source inverter switches, natural zero voltage switching (Zero Voltage Switching, ZVS) is provided. The role of non-dissipative capacitive switching snubbers may be performed by the MOSFET own output capacitance. The essence of this algorithm is to create a delay between the moment of forced switching on of the main key of the primary link and the moment of forced switching off of the transistor of the secondary link. There is also a small interval of energy return to the input source. Adjustment of the converter for the implementation of MPRT can be carried out by adjusting the ratio of the durations of the conduction state of the main and additional switches of the primary stage relative to the duration of the half-period of the frequency conversion. The types of switches were selected, static losses were estimated, simulation modeling was carried out in the MATLAB/Simulink environment, which confirmed the theoretical conclusions and the presence of soft switching modes.
El extracto metanólico de <i>Tillandsia recurvata</i> reduce los niveles de glucosa, triglicéridos y colesterol en sangre
Authors: Garcia-Campoy Abraham H. , Perez Gutierrez Rosa M. , Garcia Báez Efrén V. , Muñiz-Ramírez Alethia 
Published: September 2024

Background: Tillandsia recurvata collected in San Luis Potosí does not have studies focused on its use as an adjuvant in treating diabetes mellitus.
Questions and / or Hypotheses: Will Tillandsia recurvata L. (Bromeliaceae) have antidiabetic activity in vitro and in vivo?
Studied species: Tillandsia recurvata L. (Bromeliaceae)
Study site and dates: T. recurvata was collected in Guadalcázar municipality, San Luis Potosí, México, in December 2021.
Methods: The antidiabetic potential of Tillandsia recurvata methanol extract (TRM) was evaluated using in vitro and in vivo models, and its secondary metabolite content was analyzed using Gas chromatography-mass spectrometry.
Results: Results demonstrate that extract reduces blood glucose, triglyceride, and cholesterol levels in vivo. In addition, in vitro tests showed that extract diminished the formation of advanced glycation end products, methylglyoxal concentrations, and glycosylated hemoglobin levels. Gas chromatography-mass spectrometry analysis identified several compounds in the extract, including 2-methylbenzaldehyde, 4-hydroxy-2-methylacetophenone, 3',5' dimethoxyacetophenone, pentanoic acid, palmitic acid, linoleic acid, phytol, margaric acid, oleamide, cis-11-eicosenamide, stearic acid, 13-docosenamide, (Z), campesterol, and β-sitosterol.
Conclusions: These results highlight the potential of T. recurvata collected in San Luis Potosi as an adjuvant in treatment of diabetes mellitus.
Intrasubject variability of acoustic absorbance in adults – pilot study
Authors: Grzesiuk Patrycja , Kochanek Krzysztof , Jędrzejczak Wiesław W. 
Published: September 2024

WprowadzenieTympanometria szerokopasmowa (ang. <i>wideband tympanometry</i>, WBT) to relatywnie nowa metoda badania ucha środkowego cechująca się większymi możliwościami badawczymi niż klasyczna tympanometria. Jednak do chwili obecnej nie ustalono zakresów wartości pomiarów, które pozwoliłyby jednoznacznie odróżniać normę od patologii. Dlatego też aby WBT mogło być efektywnie stosowane klinicznie, wymaga wciąż badań nad czynnikami, które mogą wpływać na dokładność tej metody. Celem niniejszej pracy było sprawdzenie powtarzalności pomiarów WBT.Materiał i metodyGrupa badawcza składała się z 4 mężczyzn i 7 kobiet, w wieku 22–53 lata, niezgłaszających większych problemów ze słuchem. Wśród narzędzi badawczych znalazły się: kwestionariusz wywiadu oraz arkusz samooceny słuchu. Badanie właściwe przeprowadzono za pomocą sprzętu InteracousticTitan. U każdej osoby badanej wykonano 10 pomiarów w odstępie 7-dniowym.WynikiPrzebiegi wykresów absorbancji prezentowały dużą zmienność śród- jak i międzyosobniczą. Minimalny rozrzut wyników absorbancji wynosił około 0,06, a maksymalny sięgał około 0,16. Obserwując wyniki poszczególnych osób, można było zaobserwować, że u większości z nich najmniejszy rozrzut wyników absorbancji uzyskiwany był w zakresie niskich częstotliwości, a największy – w zakresie średnich i wysokich.WnioskiDzięki uzyskanym wynikom można stwierdzić, że rozrzut wyników absorbancji jest zmienny w zależności od częstotliwości. Można także zauważyć zróżnicowanie międzyosobnicze.
. Prevalence and prognostic mutation V600E in the BRAF gene in stage I cutaneous melanoma
Authors: Titov K. S. , Sorokina M. V. , Grekov D. N. , Lebedev S. S. 
Published: September 2024

Introduction. Melanoma is one of the most aggressive skin tumors, which occurs against the background of malignant transformation and proliferation of melanocytes. Risk factors for the development of cutaneous melanoma are solar radiation and duration of exposure, old age, individual patient characteristics (light skin, a large number of nevi, including atypical ones, family history) and others. Research in recent years shows that this disease is associated with a number of genetic changes, both congenital and acquired.Aim. To study the frequency of occurrence and prognostic significance of the V600E mutation in the BRAF gene in stage I skin melanoma.Materials and methods. The study was retrospective in nature and included 88 patients with stage I cutaneous melanoma (pT1–2aN0M0). All patients underwent a sentinel lymph node biopsy and no metastases were detected in it (pN0). All patients underwent molecular genetic analysis of the tumor to identify the V600E mutation in the BRAF gene with further assessment of the effect on the progression of early skin melanoma in cases of its detection.Results. The median follow-up time for patients was 32.5 (12–214) months. In 25 (28.4 %) patients of the total sample, the V600E mutation in the BRAF gene was detected. Melanoma progression during follow-up occurred in 23.9 % of patients: 44 % with the V600E mutation in the BRAF gene and 15.9 % without it (p = 0.012). In patients with this mutation, regional metastasis was more often observed, with a predominant localization of distant metastases in the bones. Survival rates were significantly higher in patients without a mutation in the BRAF gene: 1-year disease-free survival of patients without a mutation in this gene was 95 %, 3-year – 87%, 5-year – 65 %, in patients with this mutation – 84, 57 and 37 % respectively. According to the results of Cox regression analysis, in the presence of a mutation in the BRAF gene, there was an increase in the risk of progression to stage I cutaneous melanoma by 2.973 times (p = 0.016).Conclusion. The V600E mutation in the BRAF gene occurs in 28.4 % of patients with stage I cutaneous melanoma and is an unfavorable prognostic factor for disease progression.