bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxytocin and the emergence of individual differences in the social regulation of stress
Authors: Chen Frances S. , Heinrichs Markus , Johnson Susan C. 
Published: August 2017

AbstractThe ability to use social support to regulate stress is critical to mental and physical health. Here, we posit that the oxytocin system contributes to the variability in individual responses to social support. We first review the evidence that oxytocin is related to both social functioning and stress regulation. We focus on results from molecular genetics suggesting that individual variations in both of these functions are associated with natural variations in the oxytocin receptor gene (OXTR). We then describe research that exploits this natural variation to directly and experimentally test relationships between the oxytocin system, social support, and stress regulation in both infants and adults. On the basis of our findings, we propose a novel theoretical model of how biological processes might interact with psychological beliefs about relationships—even in infants—to affect long‐term patterns of social regulation of stress.
Assortative mating and couple similarity: Patterns, mechanisms, and consequences
Authors: Luo S. 
Published: August 2017

AbstractAssortative mating refers to the tendency of two partners' characteristics to be matched in a systematic manner, usually in the form of similarity. Mating with a similar partner has profound implications at the species, societal, and individual levels. This article provides a comprehensive review of research on couple similarity since 1980s. The review begins with the general patterns and trends observed in couple similarity on a range of domains including demographic variables, physical/physiological characteristics, abilities, mental well‐being, habitual behaviors, attitudes, values, and personality. Next the bulk of the review focuses on analyses of 4 mechanisms leading to similarity: initial active choice, mating market operation, social homogamy, and convergence. Specific future research avenues are outlined to improve understanding of these mechanisms. Finally, the review discusses genetic, social, and psychological consequences of couple similarity.
Knowledge, risk perception and mitigation measures towards Ebola virus disease by potentially exposed bushmeat handlers in north‐central Nigeria: Any critical gap?
Authors: Alhaji N. B. , Yatswako S. , Oddoh E. Y. 
Published: August 2017

SummaryThe bushmeat industry has been a topic of increasing importance among public health officials for its influence on zoonotic diseases transmission, such as Ebola virus disease (EVD), a rare and severe infectious disease of humans and non‐human primates. This survey assessed knowledge/awareness, risk perceptions and mitigation practices towards EVD among bushmeat handlers in north‐central Nigeria. These characteristics are premise to level of preparedness against appropriate risk prevention and control. A questionnaire‐based cross‐sectional study was conducted between January and December 2015 on 395 bushmeat handlers. Descriptive and analytical statistical analyses were performed using Epi‐Info 3.5.3 software, and p < 0.05 was considered statistical significance in all analyses. Mean age of respondents was 40.9 ± 10.7 years, and most (30.4%) of them were in the age group 40–49 years. Majority (82.8%) of them were males, while most (47.9%) do not possess formal education. Bushmeat hunters, vendors and consumers constituted 17.2%, 28.1% and 54.7% of the respondents, respectively. A majority (85.6%) of the participants had heard about EVD. Bushmeat vendors were more likely (OR 1.96; 95% CI: 1.05–3.65) to have satisfactory knowledge than the hunters. Handlers with tertiary education were more likely (OR 3.22; 95% CI: 1.56–6.67) to possess significant satisfactory knowledge/awareness about EVD. Also, vendors were more likely (OR 1.85; 95% CI: 1.01–3.42) to practice satisfactory mitigation measures than the hunters. Only handlers with tertiary education were more likely (OR 2.48; 95% CI: 1.26–4.89) to significantly practice satisfactory mitigation measures against EVD. Although most of the handlers possessed significant knowledge/awareness about EVD, few applied mitigation measures against its infection, which is the challenging gap. There is a need for collaborations between the public health, veterinary and wildlife authorities in the provision of health information to bushmeat handlers on better management of emerging and re‐emerging zoonotic viral diseases of wildlife origin.
Vitamin D3 Supplementation Does Not Improve Sprint Performance in Professional Rugby Players: A Randomized, Placebo-Controlled, Double-Blind Intervention Study
Authors: Fairbairn Kirsty A. , Ceelen Ingrid J.M. , Skeaff C. Murray , Cameron Claire M. , Perry Tracy L. 
Published: August 2017

Vitamin D insufficiency is common in athletes and may lower physical performance. Many cross-sectional studies associate vitamin D status with physical performance in athletes; however, there have been few prospective randomized controlled trials with adequate statistical power to test this relationship, and none in the southern hemisphere. Thus, a prospective double-blind, randomized, placebo-controlled intervention trial was conducted involving 57 professional rugby union players in New Zealand. Participants were randomized to receive 50,000 IU of cholecalciferol (equivalent to 3,570 IU/day) or placebo once every two weeks over 11–12 weeks. Serum 25(OH)D concentrations and physical performance were measured at baseline, weeks 5–6, and weeks 11–12. Mean (SD) serum 25(OH)D concentrations for all participants at baseline was 94 (18) nmol/L, with all players above 50 nmol/L. Vitamin D supplementation significantly increased serum 25(OH)D concentrations compared to placebo, with a 32 nmol/L difference between groups at 11–12 weeks (95% CI, 26–38; p < 0.001). Performance in five of the six tests at study completion, including the primary outcome variable of 30-m sprint time, did not differ between the vitamin D supplemented and placebo groups (p > 0.05). Performance on the weighted reverse-grip chin up was significantly higher in players receiving vitamin D compared with placebo, by 5.5 kg (95% CI, 2.0–8.9; p = 0.002). Despite significantly improving vitamin D status in these professional rugby union players, vitamin D supplementation had little impact on physical performance outcomes. Thus, it is unlikely that vitamin D supplementation is an ergogenic aid in this group of athletes.
Reliability of Urinary Dehydration Markers in Elite Youth Boxers
Authors: Zubac Damir , Cular Drazen , Marusic Uros 
Published: August 2017

Purpose: To determine the reliability and diagnostic accuracy of noninvasive urinary dehydration markers in field-based settings on a day-to-day basis in elite adolescent amateur boxers. Methods: Sixty-nine urine samples were collected daily from 23 athletes (17.3 ± 1.9 y) during their weight-stable phase and analyzed by field and laboratory measures of hydration status. Urine osmolality (UOSM), urine specific gravity (USG), total protein content (TPC), and body-mass stability were evaluated to determine fluid balance and hydration status. Overall macronutrient and water intake were determined using dietary records. According to their anthropometric characteristics, athletes were assigned into 2 groups: lightweight (LWB) and heavyweight (HWB) boxers. Results: Data presented on UOSM demonstrated a uniform increment by 11.2% ± 12.8% (LWB) and 19.9% ± 22.7% (HWB) (P < .001) over the course of the study, even during the weight-stable phase (body mass, ICC = .99) and ad libitum fluid intake (42 ± 4 mL · kg−1 · d−1). The intraclass correlation coefficients (ICCs) ranged from .52 to .55 for USG and .38 to .52 for UOSM, further indicating inconsistency of the urinary dehydration markers. Poor correlations were found between USG and TPC metabolites (r = .27, P = .211). Conclusions: Urinary dehydration markers (both USG and UOSM) exhibit high variability and seem to be unreliable diagnostic tools to track actual body-weight loss in real-life settings. The ad libitum fluid intake was apparently inadequate to match acute fluid loss during and after intense preparation. The applicability of a single-time-point hydration-status assessment concept may preclude accurate assessment of actual body-weight deficits in youth boxers.
The Role of the Rating-of-Perceived-Exertion Template in Pacing
Authors: Schallig Wouter , Veneman Tim , Noordhof Dionne A. , Rodríguez-Marroyo José A. , Porcari John P. , de Koning Jos J. , Foster Carl 
Published: August 2017

The rating-of-perceived-exertion (RPE) template is thought to regulate pacing and has been shown to be very robust in different circumstances. Purpose: The primary purpose was to investigate whether the RPE template can be manipulated by changing the race distance during the course of a time trial. The secondary purpose was to study how athletes cope with this manipulation, especially in terms of the RPE template. Method: Trained male subjects (N = 10) performed 3 cycling time trials: a 10-km (TT10), a 15-km (TT15), and a manipulated 15-km (TTman). During the TTman, subjects started the time trial believing that they were going to perform a 10-km time trial. However, at 7.5 km they were told that it was a 15-km time trial. Results: A significant main effect of time-trial condition on RPE scores until kilometer 7.5 was found (P = .016). Post hoc comparisons showed that the RPE values of the TT15 were lower than the RPE values of the TT10 (difference 0.60; CI95% 0.11, 1.0) and TTman (difference 0.73; CI95% 0.004, 1.5). After the 7.5 km, a transition phase occurs, in which an interaction effect is present (P = .011). After this transition phase, the RPE values of TTman and TT15 did not statistically differ (P = 1.00). Conclusions: This novel distance-endpoint manipulation demonstrates that it is possible to switch between RPE templates. A clear shift in RPE during the TTman is present between the RPE templates of the TT10 and TT15. The shift strongly supports suggestions that pacing is regulated using an RPE template.
Comparison of the Effects of Performance Level and Sex on Sprint Performance in the Biathlon World Cup
Authors: Luchsinger Harri , Kocbach Jan , Ettema Gertjan , Sandbakk Øyvind 
Published: August 2017

Biathlon is an Olympic sport combining cross-country skiing with the skating technique and rifle shooting. The sprint (7.5 km for women and 10 km for men) includes 2 shootings between 3 laps of skiing. The aims of the current study were to compare biathletes of different performance levels and sex on total race time and performance-determining factors of sprint races in the biathlon World Cup. The top-10 performers (G1-10) and results in ranks 21–30 (G21-30) in 47 sprint races during the 2011–12 to 2015–16 World Cup seasons were compared regarding total race time, course time, shooting time, range time, shooting performance (rate of hits), and penalty time. G21-30 men and women were on average 3–5% behind G1-10 in total race time, in which course time accounted for 59–65% of the overall performance difference, followed by 31–35% explained by penalty time. The remainder (ie, 4–6%) was explained by differences in shooting time and range time. The G1-10 women exhibited on average 12% slower speeds than the G1-10 men, and course time accounted for 93% of the total time difference of 13% between sexes. The average total hit rates were 92–93% among the G1-10 and 85% among the G21-30 in both sexes. In total, men shot on average 6 s faster than women. Course time is the most differentiating factor for overall biathlon performance between performance levels and sex in World Cup races. No sex difference in shooting performance was found.
Active Ankle Circumduction to Identify Mobility Deficits in Subacute Ankle Sprain Patients
Authors: Theurillat Clément , Punt Ilona , Armand Stéphane , Bonnefoy-Mazure Alice , Allet Lara 
Published: August 2017

Assessment of ankle mobility is complex and of clinical relevance after an ankle sprain. This study develops and tests a biomechanical model to assess active ankle circumduction and its reliability. The model was then applied to compare individuals’ ankle mobility between injured and noninjured ankles after a sprain episode. Twenty patients with subacute unilateral ankle sprain were assessed at 4 weeks and 10 weeks after the injury. They underwent a clinical exam and an ankle circumduction test during which the kinematics were recorded with an optoelectronic device. A biomechanical model was applied to explore ankle kinematics. Reliability of the ankle circumduction tests were good to excellent (ICC of 0.55–0.89). Comparison between noninjured and injured ankles showed a mobility deficit of the injured ankle (dorsiflexion = −27.4%, plantar flexion = −25.9%, eversion = −27.2%, and inversion = −11.6%). The model allows a graphical representation of these deficits in 4 quadrants. Active ankle circumduction movement can be reliably assessed with this model. In addition, the graphical representation allows an easy understanding of the mobility deficits which were present in all 4 quadrants in our cohort of patients with subacute ankle sprain.
Sociodemographic Moderators of Environment–Physical Activity Associations: Results From the International Prevalence Study
Authors: Perez Lilian G. , Conway Terry L. , Bauman Adrian , Kerr Jacqueline , Elder John P. , Arredondo Elva M. , Sallis James F. 
Published: August 2017

Background: Associations between the built environment and physical activity (PA) may vary by sociodemographic factors. However, such evidence from international studies is limited. This study tested the moderating effects of sociodemographic factors on associations between perceived environment and self-reported total PA among adults from the International Prevalence Study. Methods: Between 2002 and 2003, adults from 9 countries (N = 10,258) completed surveys assessing total PA (International Physical Activity Questionnaire-short), perceived environment, and sociodemographics (age, gender, and education). Total PA was dichotomized as meeting/not meeting (a) high PA levels and (b) minimum PA guidelines. Logistic models tested environment by sociodemographic interactions (24 total). Results: Education and gender moderated the association between safety from crime and meeting high PA levels (interaction P < .05), with inverse associations found only among the high education group and men. Education and gender also moderated associations of safety from crime and the presence of transit stops with meeting minimum PA guidelines (interaction P < .05), with positive associations found for safety from crime only among women and presence of transit stops only among men and the high education group. Conclusions: The limited number of moderating effects found provides support for population-wide environment–PA associations. International efforts to improve built environments are needed to promote health-enhancing PA and maintain environmental sustainability.
Immunogenicity of Nontypeable Haemophilus influenzae Outer Membrane Vesicles and Protective Ability in the Chinchilla Model of Otitis Media
Authors: Winter Linda E. , Barenkamp Stephen J. 
Published: August 2017

ABSTRACT

Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are enriched in several outer membrane components, including major and minor outer membrane proteins and lipooligosaccharide. We assessed the functional activity of nontypeable
Haemophilus influenzae
(NTHi) OMV-specific antisera and the protective ability of NTHi OMVs as vaccine antigens in the chinchilla otitis media model. OMVs were purified from three HMW1/HMW2-expressing NTHi strains, two of which were also engineered to overexpress Hia proteins. OMV-specific antisera raised in guinea pigs were assessed for their ability to mediate killing of representative NTHi in an opsonophagocytic assay. The three OMV-specific antisera mediated killing of 18 of 65, 24 of 65, and 30 of 65 unrelated HMW1/HMW2-expressing NTHi strains. Overall, they mediated killing of 39 of 65 HMW1/HMW2-expressing strains. The two Hia-expressing OMV-specific antisera mediated killing of 17 of 25 and 14 of 25 unrelated Hia-expressing NTHi strains. Overall, they mediated killing of 20 of 25 Hia-expressing strains. OMVs from prototype NTHi strain 12 were used to immunize chinchillas and the course of middle ear infection was monitored following intrabullar challenge with the homologous strain. All control animals developed culture-positive otitis media, as did two of three HMW1/HMW2-immunized animals. All OMV-immunized animals, with or without supplemental HMW1/HMW2 immunization, were completely protected against otitis media. NTHi OMVs are the first immunogens examined in this model that provided complete protection with sterile immunity after NTHi strain 12 challenge. These data suggest that NTHi OMVs hold significant potential as components of protective NTHi vaccines, possibly in combination with HMW1/HMW2 proteins.
Evaluation of a Novel Reporter Virus Neutralization Test for Serological Diagnosis of Zika and Dengue Virus Infection
Authors: Shan Chao , Ortiz Daniel A. , Yang Yujiao , Wong Susan J. , Kramer Laura D. , Shi Pei-Yong , Loeffelholz Michael J. , Ren Ping 
Published: August 2017

ABSTRACT
Currently, the laboratory diagnosis of Zika virus (ZIKV) infection is primarily through the detection of ZIKV RNA or antibodies against ZIKV proteins. The detection of viral RNA is highly sensitive and specific, but periods of viremia and viruria are brief, limiting the utility of ZIKV RNA assays. Instead, most ZIKV infections are diagnosed serologically, using an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for screening, followed by a confirmatory plaque reduction neutralization test (PRNT). Typical turnaround times vary, due to assay incubation periods and a lack of clinical laboratories performing these tests. Recently, a novel luciferase-ZIKV- and -dengue virus (DENV)-based serological assay, which considerably improves the turnaround times and throughput for ZIKV diagnosis, was described. Using the traditional PRNT as a reference method, we evaluated the performance characteristics of the reporter virus neutralization test (RVNT) with 258 clinical serum specimens. The ZIKV RVNT produced primary ZIKV screening and secondary confirmation results in 4 days, with 100% reproducibility. As a screening assay, the ZIKV RVNT displayed excellent diagnostic accuracy, sensitivity, and specificity of 98.2%, 100%, and 98.1%, respectively. As a confirmatory assay, the ZIKV RVNT titers displayed 93.1% agreement with the traditional ZIKV PRNT titers. Overall, the RVNT accurately and reliably detects neutralizing antibodies in patient serum specimens, with improved turnaround times, and can be used for the serological detection of ZIKV infections. Due to the homogeneous 96-well format, the RVNT has also significantly improved the assay throughput to allow testing of a large number of specimens in a single run.
Molecular Function Analysis of Rabies Virus RNA Polymerase L Protein by Using an L Gene-Deficient Virus
Authors: Nakagawa Kento , Kobayashi Yuki , Ito Naoto , Suzuki Yoshiyuki , Okada Kazuma , Makino Machiko , Goto Hideo , Takahashi Tatsuki , Sugiyama Makoto 
Published: August 2017

ABSTRACT

While the RNA-dependent RNA polymerase L protein of rabies virus (RABV), a member of the genus
Lyssavirus
of the family
Rhabdoviridae
, has potential to be a therapeutic target for rabies, the molecular functions of this protein have remained largely unknown. In this study, to obtain a novel experimental tool for molecular function analysis of the RABV L protein, we established by using a reverse genetics approach an L gene-deficient RABV (Nishi-ΔL/Nluc), which infects, propagates, and correspondingly produces NanoLuc luciferase in cultured neuroblastoma cells transfected to express the L protein.
trans
-Complementation with wild-type L protein, but not that with a functionally defective L protein mutant, efficiently supported luciferase production by Nishi-ΔL/Nluc, confirming its potential for function analysis of the L protein. Based on the findings obtained from comprehensive genetic analyses of L genes from various RABV and other lyssavirus species, we examined the functional importance of a highly conserved L protein region at positions 1914 to 1933 by a
trans
-complementation assay with Nishi-ΔL/Nluc and a series of L protein mutants. The results revealed that the amino acid sequence at positions 1929 to 1933 (NPYNE) is functionally important, and this was supported by other findings that this sequence is critical for binding of the L protein with its essential cofactor, P protein, and thus also for L protein's RNA polymerase activity. Our findings provide useful information for the development of an anti-RABV drug targeting the L-P protein interaction.

IMPORTANCE
To the best of our knowledge, this is the first report on the establishment of an L gene-deficient, reporter gene-expressing virus in all species of the order
Mononegavirales
, also highlighting its applicability to a
trans
-complementation assay, which is useful for molecular function analyses of their L proteins. Moreover, this study revealed for the first time that the NPYNE sequence at positions 1929 to 1933 in the RABV L protein is important for L protein's interaction with the P protein, consistent with and extending the results of a previous study showing that the P protein-binding domain in the L protein is located in its C-terminal region, at positions 1562 to 2127. This study indicates that the NPYNE sequence is a promising target for the development of an inhibitor of viral RNA synthesis, which has high potential as a therapeutic drug for rabies.