bluuebunny/crossref_metadata_2025_split huggingface dataset:
The first characterization of Escherichia coli isolates recovered from three wastewater treatment plants in Romania
Authors: Polianciuc Svetlana Iuliana , Colobatiu Liora , Duma Mihaela , Mihaiu Marian , Loghin Felicia 
Published: September 2024

ABSTRACT
The global growth of antimicrobial resistance (AMR) raises significant challenges to public health, necessitating comprehensive surveillance and intervention strategies. This study investigates the prevalence and resistance profiles of Escherichia coli isolated from three different wastewater treatment plants (WWTPs) in Romania during the warm season of 2023. Through systematic sampling and subsequent microbiological and molecular analyses, we identified a 50% prevalence of E. coli in wastewater samples, with a concerning 83.34% of isolates displaying resistance to multiple antibiotics. The resistance was notably high against ampicillin, ticarcillin/clavulanic acid, and cefalotin, with observed multidrug resistance suggesting a robust presence of antibiotic-resistant bacteria (ARB) within treated effluents. Molecular characterization confirmed the presence of multiple antibiotic resistance genes (ARGs), including β-lactamase producers and markers for tetracycline and sulphonamide resistance. These findings underscore the critical role of WWTPs as both reservoirs and potential dissemination points for ARB and ARGs, highlighting the need for integrated surveillance systems and enhanced wastewater treatment protocols to mitigate the spread of AMR. This study emphasizes the necessity of bridging clinical and environmental monitoring to develop effective public health strategies against the threat of antibiotic resistance.
Integrating Network Pharmacology, Bioinformatics, and Mendelian Randomization Analysis to Identify Hub Targets and Mechanisms of Kunkui Baoshen Decoction in Treating Diabetic Kidney Disease
Authors: Song Siyuan , Yu Jiangyi 
Published: September 2024

Objective:
To uncover the potential hub targets of Kunkui Baoshen Decoction (KKBS) in alleviating
Diabetic Kidney Disease (DKD).

Methods:
Targets associated with KKBS and DKD were curated from TCMSP, GeneCards, OMIM, and Dis-
GeNET databases. Common targets were identified through intersection analysis using a Venn diagram. Employing
the "Drug-component-target" approach and constructing a Protein-protein Interaction (PPI) network,
pivotal components and hub targets involved in KKBS's therapeutic action against DKD were identified.
Functional enrichment and Gene Set Enrichment Analysis (GSEA) elucidated the potential mechanisms of
these hub targets. Molecular docking simulations validated binding interactions. Subsequently, hub targets
were validated using independent cohorts and clinical datasets. Immune cell infiltration in DKD samples was
assessed using ESTIMATE, CIBERSORT, and IPS algorithms. A nomogram was developed to predict DKD
prevalence. Finally, causal relationships between hub targets and DKD were explored through Mendelian randomization
(MR) analysis at the genetic level.

Results:
Jaranol, isorhamnetin, nobiletin, calycosin, and quercetin emerged as principal effective components
in KKBS, with predicted modulation of the PI3K/Akt, MAPK, HIF-1, NF-kB, and IL-17 signaling pathways.
The hub targets in the PPI network include proteins involved in regulating podocyte autophagy and apoptosis,
managing antioxidant stress, contributing to insulin resistance, and participating in extracellular matrix deposition
in DKD. Molecular docking affirmed favorable binding interactions between principal components and
hub targets. Validation efforts across cohorts and databases underscored the potential of hub targets as DKD
biomarkers. Among 20 model algorithms, the Extra Tree model yielded the largest Area Under the Curve
(AUC) in receiver operating characteristic (ROC) analysis. MR analysis elucidated that the targets related to
antioxidant stress had a positive impact on DKD, while the target associated with renal tubular basement
membrane degradation had a negative impact.

Conclusion:
Integration of Network Pharmacology, Bioinformatics, and MR analysis unveiled the capacity of
KKBS to modulate pivotal targets in the treatment of DKD.
Metabolic Pathway of Osilodrostat in Equine Urine Established through High-resolution Mass Spectrometric Characterization for Doping Control
Authors: Ishii Hideaki , Shigematsu Ryo , Takemoto Shunsuke , Ishikawa Yuhiro , Mizobe Fumiaki , Nomura Motoi , Arima Daisuke , Kunii Hirokazu , Yuasa Reiko , Yamanaka Takashi , Tanabe Sohei , Nagata Shun-ichi , Yamada Masayuki , Leung Gary Ngai-Wa 
Published: September 2024

Objective:
Osilodrostat, used to treat Cushing's disease, exhibits an anabolic effect, leading to its
classification as a prohibited substance in horseracing and equestrian sports. This study reports the characterization
of osilodrostat metabolites in horse urine and elucidates its metabolic pathways for the first time for
doping control purposes.

Methods:
Osilodrostat was administered nasoesophageally to four thoroughbreds (one gelding and three
mares) at a dose of 50 mg each. Potential metabolites were extensively screened via our developed generic
approach employing differential analysis to identify metabolites. Specifically, high-resolution mass spectral
data were compared between pre- and post-administration samples on the basis of criteria of fold-changes of
peak areas and their P values. Potential metabolite candidates were further identified through mass spectral
interpretations using product ion scan data.

Results:
A total of 37 metabolites were identified after comprehensive analysis. Osilodrostat was predominantly
metabolized into a mono-hydroxylated form M1c (~40%) alongside osilodrostat glucuronide M2
(~17%). Given their longest detection time (2 weeks after administration) and the identification of several
conjugates of osilodrostat and M1c, including a novel conjugate of riburonic acid, we recommend monitoring
both osilodrostat and M1c after hydrolysis during the screening stage. However, only osilodrostat can be used
for confirmation because of the availability of a reference material.

Conclusion:
It is advisable to screen for both osilodrostat and its mono-hydroxylated metabolite M1c to effectively
monitor horse urine for the potential misuse or abuse of osilodrostat. For suspicious samples, confirmation
of osilodrostat using its reference material is required.
The Effect of Service Quality and Perceived Value on Customer Satisfaction and Its Impact on Customer Loyalty
Authors: Hadiputro Mohamad Fauzan Muzhaffar , Rofiaty Rofiaty 
Published: September 2024

This research aims to determine the effect of service quality and perceived value on customer satisfaction and its impact on customer loyalty at Araya Golf Course. This research’s type is causal analysis research. A sample size of 100 respondents were used. The respondents were customers at Araya Golf Course. Likert scale was utilized in the research instrument and path analysis model was employed. The instrument test in this study is the validity test and the reliability test. The test results show a positive and significant effect of Service Quality on Customer Loyalty through Customer Satisfaction. The results of this research also show that there is a positive and significant effect of Perceived Value on Customer Loyalty through Customer Satisfaction.

Abstrak
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh kualitas pelayanan dan nilai yang dirasakan terhadap kepuasan pelanggan dan pengaruhnya terhadap loyalitas pelanggan di Lapangan Golf Araya. Jenis penelitian ini adalah penelitian analisis kausal. Penelitian ini menggunakan ukuran sampel sebanyak 100 Responden. Responden yang dipilih dalam penelitian ini adalah seluruh pelanggan di Lapangan Golf Araya. Instrumen penelitian ini menggunakan skala Likert. Dalam penelitian ini digunakan model analisis jalur. Uji instrumen dalam penelitian ini adalah uji validitas dan uji reliabilitas. Hasil pengujian menunjukkan bahwa terdapat pengaruh positif dan signifikan Service Quality terhadap Loyalitas Pelanggan melalui Kepuasan Pelanggan. Hasil penelitian ini juga menunjukkan bahwa terdapat pengaruh positif dan signifikan Perceived Value terhadap Loyalitas Pelanggan melalui Kepuasan Pelanggan.
Wearable Smartphone-Based Multisensory Feedback System for Torso Posture Correction: Iterative Design and Within-Subjects Study
Authors: Pereira Amanda Polin , Machado Neto Olibario Jose , Elui Valeria Meirelles Carril , Pimentel Maria da Graca Campos 
Published: September 2024

Background
The prevalence of stroke is high in both males and females, and it rises with age. Stroke often leads to sensor and motor issues, such as hemiparesis affecting one side of the body. Poststroke patients require torso stabilization exercises, but maintaining proper posture can be challenging due to their condition.

Objective
Our goal was to develop the Postural SmartVest, an affordable wearable technology that leverages a smartphone's built-in accelerometer to monitor sagittal and frontal plane changes while providing visual, tactile, and auditory feedback to guide patients in achieving their best-at-the-time posture during rehabilitation.

Methods
To design the Postural SmartVest, we conducted brainstorming sessions, therapist interviews, gathered requirements, and developed the first prototype. We used this initial prototype in a feasibility study with individuals without hemiparesis (n=40, average age 28.4). They used the prototype during 1-hour seated sessions. Their feedback led to a second prototype, which we used in a pilot study with a poststroke patient. After adjustments and a kinematic assessment using the Vicon Gait Plug-in system, the third version became the Postural SmartVest. We assessed the Postural SmartVest in a within-subject experiment with poststroke patients (n=40, average age 57.1) and therapists (n=20, average age 31.3) during rehabilitation sessions. Participants engaged in daily activities, including walking and upper limb exercises, without and with app feedback.

Results
The Postural SmartVest comprises a modified off-the-shelf athletic lightweight compression tank top with a transparent pocket designed to hold a smartphone running a customizable Android app securely. This app continuously monitors sagittal and frontal plane changes using the built-in accelerometer sensor, providing multisensory feedback through audio, vibration, and color changes. Patients reported high ratings for weight, comfort, dimensions, effectiveness, ease of use, stability, durability, and ease of adjustment. Therapists noted a positive impact on rehabilitation sessions and expressed their willingness to recommend it. A 2-tailed t-test showed a significant difference (P<.001) between the number of the best-at-the-time posture positions patients could maintain in 2 stages, without feedback (mean 13.1, SD 7.12) and with feedback (mean 4.2, SD 3.97), demonstrating the effectiveness of the solution in improving posture awareness.

Conclusions
The Postural SmartVest aids therapists during poststroke rehabilitation sessions and assists patients in improving their posture during these sessions.
Leveraging Chatbots to Combat Health Misinformation for Older Adults: Participatory Design Study
Authors: Peng Wei , Lee Hee Rin , Lim Sue 
Published: September 2024

Background
Older adults, a population particularly susceptible to misinformation, may experience attempts at health-related scams or defrauding, and they may unknowingly spread misinformation. Previous research has investigated managing misinformation through media literacy education or supporting users by fact-checking information and cautioning for potential misinformation content, yet studies focusing on older adults are limited. Chatbots have the potential to educate and support older adults in misinformation management. However, many studies focusing on designing technology for older adults use the needs-based approach and consider aging as a deficit, leading to issues in technology adoption. Instead, we adopted the asset-based approach, inviting older adults to be active collaborators in envisioning how intelligent technologies can enhance their misinformation management practices.

Objective
This study aims to understand how older adults may use chatbots’ capabilities for misinformation management.

Methods
We conducted 5 participatory design workshops with a total of 17 older adult participants to ideate ways in which chatbots can help them manage misinformation. The workshops included 3 stages: developing scenarios reflecting older adults’ encounters with misinformation in their lives, understanding existing chatbot platforms, and envisioning how chatbots can help intervene in the scenarios from stage 1.

Results
We found that issues with older adults’ misinformation management arose more from interpersonal relationships than individuals’ ability to detect misinformation in pieces of information. This finding underscored the importance of chatbots to act as mediators that facilitate communication and help resolve conflict. In addition, participants emphasized the importance of autonomy. They desired chatbots to teach them to navigate the information landscape and come to conclusions about misinformation on their own. Finally, we found that older adults’ distrust in IT companies and governments’ ability to regulate the IT industry affected their trust in chatbots. Thus, chatbot designers should consider using well-trusted sources and practicing transparency to increase older adults’ trust in the chatbot-based tools. Overall, our results highlight the need for chatbot-based misinformation tools to go beyond fact checking.

Conclusions
This study provides insights for how chatbots can be designed as part of technological systems for misinformation management among older adults. Our study underscores the importance of inviting older adults to be active co-designers of chatbot-based interventions.
Pengaruh Brand Awareness,Trust Dan Relationship Terhadap Keputusan Membeli Pelanggan RM Putra Minang Group
Authors: Oktaviar Chairiel , Arief Harefan , Viciwati Viciwati 
Published: September 2024

Penelitian ini bertujuan untuk mengetahui dan mengukur pengaruh brand awareness, trust dan relationship terhadap keputusan membeli pelanggan RM Putra Minang Group di wilayah Tangerang Selatan dan sekitarnya dan untuk mengetahui seberapa besar pangsa pasar RM Putra Minang Group sehingga dapat membangun dan menumbuhkan brand awarenes yang cukup kuat dan lebih dekat di hati masyarakat di wilayah Tangerang Selatan dan sekitarnya dibandingkan dengan rumah makan sejenis yang lebih dahulu masuk dalam bisnis rumah makan padang tersebut, Penelitian menggunakan data kuesioner dari 98 responden,data diolah menggunakan Smart-PLS dengan hasil dari penelitian peneliti mendapat kesimpulkan bahwa brand awareness, trust dan relationship berpengaruh positif dan siqnifikan terhadap keputusan membeli pelanggan RM Putra Minang Group.
Comparison of Real Response Modification Factors and Performance of SMRFS Structure with OMRFS Structure Using Pushover Method
Authors: Damayanti Friska , Muin Resmi Bestari 
Published: September 2024

The value of the earthquake response modification factor ( ) affects the results of the story drift in the SMRSF structure, where the smaller the -value, the larger the deviation and the need for longitudinal reinforcement will be more and more. For structures that do not comply with the SMRSF provisions, in some locations the need for reinforcement is less than that of structures that use the SMRSF provisions. The performance of a structure can be analyzed using the pushover method. The research was conducted with a 4-story and 8-story configuration, with soft soil and medium soil conditions and using SMRSF and OMRSF. From research analysis results, the cross-sectional dimensions of the columns and beams of the ordinary moment building structure system with soft soil type are the largest cross-sectional dimensions compared to the others. Under the provisions of ATC-40, the structure is included in the Damage Control (DC) category level, which means the building is still able to withstand the force of the earthquake, and the risk of fatalities humans are very small. The real -value of the structure with SMRSF is not much different from the in the provisions of SNI 1726:2019.
Sistem Kendali Closed Loop Pada Mesin Extruder Straw Berbasis Logika Fuzzy
Authors: Sucipto Dany , Qomaruddin Muhammad , Marwanto Arief 
Published: September 2024

Akibat panas yang berlebihan pada heater, heater mati atau panas terlalu tinggi, tetapi motor DC pada extruder tetap berjalan dan mengeluarkan resin, sehingga hasil produksi tidak sesuai dengan desain. Sehingga diperlukan sebuah methoda untuk pengendalian mesin extruder agar produksi dapat berjalan dengan baik. Penelitian dilakukan dengan mengontrol parameter operasional seperti suhu, kecepatan ekstrusi pada mesin. implementasi sistem kendali closed-loop yang mampu beradaptasi dengan perubahan dalam proses ekstrusi. Sistem ini secara otomatis mengontrol parameter operasional berdasarkan umpan balik yang diterima dari keluaran produksi. Pengujian awal menunjukkan bahwa sistem kendali ini memiliki potensi untuk meningkatkan efisiensi produksi dan menghasilkan sedotan plastik dengan kualitas yang lebih stabil. Metode logika fuzzy digunakan untuk mengatasi variabilitas dalam bahan baku dan perubahan kondisi operasional, sehingga menghasilkan hasil produksi yang lebih konsisten dan berkualitas baik. Logika fuzzy yang digunakan dalam sistem kendali closed-loop mesin extruder straw menghasilkan dampak positif dalam mengatasi variabilitas produksi. Hasil percobaan menunjukkan bahwa methoda closed loop dan fuzzy logic yang diimplementasikan pada mesin extruder ini dapat mengatasi kerusakkan produksi dengan nilai defuzifikasi 6,8 dengan nilai sedang. Sehingga kendali closed-loop dapat bekerja didua kondisi yaitu panas sedang dan tinggi. Kata kunci : Mesin extruders, kendali close loop, Logika Fuzzy
Simbolismos étnicos afirmados através do matriarcado de mulheres negras de terreiros
Authors: De Santana Marise , Sales Oliveira Viviane 
Published: September 2024

Neste artigo, embora estejamos focadas na etnicidade como forma de organização social de mulheres negras, lideranças de terreiro, que estamos chamando de Matriarcas, nosso propósito é pensar sobre como a tradição é mantida pelos simbolismos étnicos. Entendemos simbolismos étnicos, como saberes e fazeres materiais e imateriais, ensinados/aprendidos através de narrativas míticas geradoras de símbolos tradicionais e que para nós, como estudiosas dos legados africanos, nos interessa pensar sobre os conhecimentos das tradições afro-brasileiras, em especial, das mulheres que exercem o Matriarcado em muitos terreiros. Estamos falando em Matriarcado, correndo todos os possíveis riscos que esse debate traz. Estamos tomando Matriarcado para falar de mulheres, que nos aponta narrativas negras referenciadas em seus saberes, mitos e símbolos transmitidos por meio de cantigas, danças, rezas. Portanto, as Matriarcas, no movimento feito da “porteira para dentro”, mantêm as tradições refletidas nos saberes dos simbolismos étnicos ancestrais a respeito do feminino. Da “porteira para fora”, como liderança religiosa, social e cultural, lutam contra as discriminações sexistas e raciais. Neste sentido, falar sobre o conhecimento da etnicidade que a Matriarca possui, a coloca no lugar de quem tem o conhecimento mais profundo da magia e do encantamento mobilizados pelo grupo étnico ao qual pertence.
Impact of Corporate Climate Change Performance on Information Asymmetry: International Evidence
Authors: Dey Sajal Kumar , Bose Sudipta , Luo Le , Shams Syed 
Published: September 2024

ABSTRACT
In this study, we examine the association between climate change performance and information asymmetry using 6,367 firm-year observations from 2011 to 2020 across 26 countries. We find that climate change performance is negatively associated with information asymmetry, suggesting that firms with higher climate change performance tend to have lower information asymmetry. We also find that the negative association between climate change performance and information asymmetry is stronger for firms with a higher level of institutional ownership and better corporate governance. Further analyses show a more pronounced negative association between climate change performance and information asymmetry for firms domiciled in countries with stakeholder-oriented business culture, a national emissions trading scheme, and a higher level of climate change performance. Our study’s findings have significant implications for capital market participants, managers, policymakers, researchers, and practitioners worldwide in understanding the role of corporate climate change performance in the capital market.
Data Availability: All data used in this paper are publicly available from the sources stated in the paper.
JEL Classifications: G14; M14; M41; Q51; Q54.
Adrenal Dysfunction Caused by Critical Illness and Its Correction by Controlling Body Temperature: Prospective Cohort Study
Authors: Altshuler N. E. , Kruglyakov N. M. , Kutsyi M. B. , Popugaev K. A. 
Published: September 2024

BACKGROUND. Critical condition (CC) is defined as any life-threatening condition that requires support of the functions of vital organs and systems to prevent imminent death. From the point of view of the course of CC and the survival of the patient, adequate functioning of the hypothalamic-pituitary-adrenal axis (HPA) is fundamentally important. AIM OF STUDY. To improve treatment outcomes for critically ill patients requiring temperature management through early diagnosis and timely correction of adrenal dysfunction. MATERIAL AND METHODS. Of the 59 patients, 45 (76.3 %) patients were diagnosed with adrenal dysfunction upon body temperature management (BTM) caused by critical illness: group I with vascular failure (VF+); in 14 (23.7 %) patients of group II adrenal disfunction wasn’t revealed (VF-). RESULTS. In group I (VF+) there was a high SOFA score, the highest number of days from the moment of illness to admission to the intensive care unit andinitiation of BTM, the presence of liver dysfunction, and a high level of C-reactive protein. Vascular failure in patients in group I developed earlier than other organ dysfunctions and sepsis. The high level of cortisol in the blood plasma in group II (VF-) was associated with the development of corticoresistance. CONCLUSIONS. 1. At body temperature management initiation, patients with adrenal dysfunction had a statistically significant high sofa score compared to patients without adrenal dysfunction (8 (5–9); 7 (6–8), respectively, p < 0,05). 2. The number of days from the disease onset to admission to the icu and initiation of body temperature management was statistically significantly high in the group with adrenal dysfunction compared to the group without adrenal dysfunction (20 of 45 patients (44.4 %); 4 of 14 (28.6 %) respectively, p < 0.05). 3. In the development of septic shock in patients with adrenal dysfunction, the criterion for the efficacy of the therapy was stabilization of the patient’s condition: positive hemodynamic response to the introduction of hydrocortisone with reduced doses of noradrenaline and its subsequent cancellation. 4. Despite the fact that the rate of organ dysfunction, sepsis and septic shock were statistically significantly higher (p < 0.05) in the group with adrenal dysfunction and the presence of vascular insufficiency, the performed treatment (hydrocortisone administration) allowed outcomes comparable to these in patients without adrenal dysfunction to be achieved (p > 0.05).