bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing the understanding of safety and quality of patient care among medical and health sciences students in an interprofessional climate: An interventional study
Authors: Guraya Salman Yousuf , Sulaiman Nabil , Hasswan Ahmed , Salmanpour Vida Abdolhamid , Jirjees Feras Jassim , Taha Mohamed Hassan , Alamara Jennat , Kawas Sausan AL , Awad Manal , Dias Jacqueline Maria , David Leena R , Haider Mohamed Aly , Guraya Shaista Salman , Dash Nihar Ranjan , Al-Qallaf Amal , Shorbagi Sarra , Mahmoud Ibrahim 
Published: October 2024

Abstract

Background
Interprofessional education (IPE) plays an elementary role in improving healthcare outcomes by shared understanding and by reducing errors and adverse events. Unfortunately, most healthcare professionals have not received training for patient safety (PS) in an interprofessional setting, which can meet the needs of societal medical needs. This study aimed to foster the understanding of senior medical and health sciences students about PS and quality of care at the University of Sharjah in UAE.
Methods
Using a stratified random sampling technique, we recruited students from College of Medicine (CoM), College of Pharmacy (CoP), College of Dental Medicine (CDM), and College of Health Sciences (CHS) of UoS. The online synchronous real-time workshop included interactive resource sessions, breakout room discussions using real clinical cases, and pre-post surveys using a questionnaire with three domains of PS; medical professionalism, leadership in healthcare, and precision medicine.
Results
A total of 248 students and 20 facilitators participated in the workshop. CoM had the highest representation (47.5%), followed by CoP (36.5%), CHS (13.3%), and CDM (2.7%). There was a statistically improved understanding in all domains of PS across all colleges (p < 0.001). Notably, students in years four and five from CoM and CoP showed significant improvement in their perceptions than other colleges (p < 0.001). The participants encountered challenges of weak internet connections, software issues, technical failures, and power outages during the workshop.
Conclusion
This study highlights a positive impact of an IPE-based interventional workshop on the students' understanding and insights of PS and quality of care. This underscores the paramount role of IPE in improving perceptions and approaches towards PS in the context of healthcare education.
HPN: A Multimodal Neural Network Model for Non-invasive HER2 Status Assessment in Breast Cancer Patients
Authors: Liu Yuhan , Ye Jinlin , He Zecheng , Wang Mingyue , Wang Changjun , Zhou Yidong , Zhang Wei 
Published: October 2024

Abstract

Background
HER2-positive breast cancer is known for its aggressive behavior and poorer prognosis in the absence of anti-HER2 therapy. Current assessments of HER2+ highlight the need for non-invasive diagnostic tools. This study introduces a multimodal approach called the HER2 Prediction Network (HPN) to noninvasively predict HER2 status, thereby supporting the precise administration of HER2-targeted therapies.
Methods
A cohort of 482 breast cancer patients were enrolled from Peking Union Medical College Hospital. HPN was developed by ResNet and Transformer, utilizing clinicopathological and ultrasound data collected from breast cancer patients. After training, this model could differentiate HER2-zero, HER2-low and HER2-positive breast cancer patient and detect HER2 status in different peritumoral regions.
Findings
The HPN demonstrated robust performance in HER2 expression identification of breast cancer patients. It achieved an Accuracy of 0.76 and an Area Under the Curve(AUC) of 0.86. Detections for different peritumoral regions have all shown favorable results(AUC1.2x =0.85, AUC1.4x =0.85 AUC1.6x =0.86).
Conclusion
The HPN provided a non-invasive method for assessing HER2 expression, thereby facilitating decision-making regarding the intervention of HER2-targeted therapy.
Replacing inorganic trace minerals with advanced chelate technology based organic trace minerals on reproductive efficiency, blood metabolites, hormonal profile, antioxidant status, and enzymes in Ghezel ewes
Authors: Vatankhah Sedigheh , Ebrahimi Marziyeh , Taghizadeh Akbar , Asadpour Reza , Nazaran Mohammad Hassan 
Published: October 2024

Abstract

The bioavailability of trace minerals (TM) in the diet are important for improving reproductive performance and fertility parameters. This trial was conducted to compare the effect of replacing inorganic trace minerals (ITM) with organic trace minerals (OTM, based on advanced chelate technology) on physiological status and reproductive performance of ewes. Accordingly, forty ewes were treated for five weeks based on four dietary groups included: 1- OTM: flushed with organic trace minerals (Bonzaplex-7), 2- ITM flushed with inorganic TM, 3- NTM flushed without TM, 4-CON: only pasture grazed. Estrus synchronization was performed based on two doses of 75 µg cloprostenol with 12 days' interval + 400 IU eCG injection. The number of follicles and pregnancy were determined by ultrasound. Blood samples were also collected during the experiment. The results of the present experiment showed higher pregnancy, lambing and twinning rates in OTM and ITM groups as compared with other groups, in which OTM was more effective in the indexes. However, follicle number were not affected by the treatments. Also, T3 and T4 as well as triglyceride were higher in OTM group as compared with other groups (P < 0.05). Serum estradiol, progesterone, and antioxidant enzymes were not affected by treatments (p > 0.05). In overall, supplementing advanced chelate technology based OTM in the flushing diet improved reproductive performance of ewes.
HSP27/IL-6 axis promotes OSCC chemoresistance of cisplatin, migration and invasion by orchestrating macrophages via a positive feedback loop
Authors: Qi Ying , Cao Juan , Jiang Mingjing , Lin Ying , Li Weibo , Li Bo 
Published: October 2024

Abstract

The mechanisms of interaction and crosstalk between tumor cells and tumor-associated macrophages (TAMs) have provided novel options for intervening in tumor progression. However, the molecular mechanisms of the interaction between the tumor cells and TAMs underlying oral squamous cell carcinoma (OSCC) invasio, migration and chemoresistance remain unclear. This study sought to specifically investigate the role of the tumor-cell-derived paracrine heat shock protein 27 (HSP27) in OSCC invasion, migration and chemoresistance and the potential molecular mechanisms of the interaction between the tumor cells and TAMs. In this study, bioinformatic analysis and IHC results demonstrated that the expression level of HSP27 was higher in the tissues of patients with advanced lymph node metastasis of OSCC than that in early stage non-metastatic patients, and that its expression level was positively correlated with the levels of multidrug resistance-associated proteins and macrophage infiltration. In vivo, Survival of low-expressing HSP27 xenograft model mice was inferior to that of controls. In vitro, TAMs-CM significantly up-regulated the expression level of HSP27 in the two types of OSCC tumor cells including CAL27 and SCC9 cells. The OSCC tumor cell-derived HSP27 regulated TAMs through paracrine mode and reduced the level of apoptosis induced by the chemotherapeutic drug cisplatin in tumor cells, thus promoting chemoresistance in OSCC. HSP27 promoted the secretion of cytokine IL-6 from TAMs, whereas TAMs-derived IL-6 up-regulated the expression of HSP27 and enhanced the chemoresistance, migration and invasion of OSCC in tumor cells through an autocrine mode and activates the β-catenin pathway during this process, additionally up-regulated the stem cell properties of tumor cells through an autocrine manner. Tumor-cell-derived paracrine HSP27 promoted OSCC invasion and migration through enhancing the epithelial–mesenchymal transition (EMT) of tumor cells via binding to toll-like receptor 4 (TLR4) on the surface of the TAMs. HSP27/TLR4 induced polarization of the TAMs to an M2-like phenotype and the secretion of IL-6 in the TAMs. Respectively, TAMs-derived IL-6 enhanced OSCC invasion and migration via autocrine HSP27/TLR4 signaling in tumor cells while IL-6 promoted the EMT of tumor cells via autocrine HSP27. Collectively, tumor-cell-derived paracrine HSP27 promoted OSCC migration, invasion and chemoresistance by orchestrating macrophage M2 polarization and IL-6 secretion from macrophages via a positive feedback loop. TAM-derived IL-6 enhanced these progressions via autocrine HSP27/IL-6 signaling in tumor cells. Targeting HSP27/IL-6 may be an effective treatment strategy for OSCC patients, and it is expected to control OSCC progression and improve its prognosis and recurrence in patients.
Diagnostic value of neutrophil-to-lymphocyte ratio, fibrinogen-to-albumin ratio and red blood cell distribution width in tuberculosis combined with bacterial infection
Authors: Fu HaiYang , Zhang Haiyun , Wang QiuBo , Zhu HuiMing 
Published: October 2024

Abstract

Objective: To investigate the clinical significance of neutrophil-to-lymphocyte ratio (NLR), fibrinogen-to-albumin ratio (FAR), and red blood cell distribution width (RDW) in pulmonary tuberculosis (PTB) combined with bacterial infection of the lung.
Metheds: 74 patients with pulmonary tuberculosis combined with bacterial infection of the lung attending the Sixth People's Hospital of Nantong City from January 2021 to December 2024 were selected as the infected group, and 96 patients with simple pulmonary tuberculosis admitted during the same period were selected as non-infected group, and the levels of NLR, FAR, and RDW in peripheral blood of the patients of the two groups were collected for determination, and NLR, FAR and RDW levels of the patients of the two groups were compared.The receiver operating characteristic (ROC) curve was used to assess the performance of the above indicators for early diagnosis of PTB combined with bacterial infection.
Results: The levels of NLR, FAR and RDW were significantly higher in the infected group compared with the non-infected group, and the differences were statistically significant (P < 0.05).The levels of NLR were positively correlated with the levels of leukocytes, C-reactive protein and D-dimer.ROC curve analysis showed that the area under the curve (AUC) for the diagnosis of pulmonary tuberculosis combined with bacterial infection by blood NLR, FAR and RDW were 0.861, 0.818, 0.799; the AUC value after the combination of the three was 0.982. The validation results showed that the diagnostic sensitivity (98.6%) and specificity (89.58%) of NLR and FAR combined with RDW were higher than those of NLR, FAR and RDW alone.
Conclusion: Combined detection of blood NLR, FAR and RDW levels has high clinical diagnostic value for diagnosing pulmonary tuberculosis combined with bacterial infection.
Prognostic significance of angiogenesis-associated molecules and immunologic characteristic in elderly patients with acute myeloid leukemia
Authors: Chen Can , Huang Yongfen , Wang Lingling , Zhang Linlin , Lu Jinbo , Cheng Yuexin , Miao Yuqing 
Published: October 2024

Abstract

Background
Neovascularization mechanisms are hyperactivated in tumors, leading to vascular dysfunction and contributing to tumor metastasis and growth. This study aims to comprehensively analyze angiogenesis-associated genes in relation to the prognosis of elderly patients with acute myeloid leukemia (AML). Methods: Leukemia gene expression data were obtained from the GSE37642 (training set) and TCGA_LAML (validation set) datasets. Angiogenesis-associated genes were identified using the GeneCards database. Univariate Cox regression and LASSO analyses were employed to identify angiogenesis-associated genes linked to AML prognosis. A prognostic signature was constructed based on the selected genes, and its biological functions were analyzed. Finally, we predicted AML drug sensitivity and evaluated differences in drug activity based on the prognostic signature. Results: Five angiogenesis-related genes associated with AML prognosis were identified: ECM1, EGLN1, FKBP5, FOXP1, and SIRT2. Kaplan-Meier analyses confirmed their prognostic value. A prognostic signature based on these genes demonstrated commendable efficacy in predicting patient outcomes. This signature was found to be an independent risk factor for AML and revealed distinct immune profiles. Furthermore, the signature was implicated in the tumor immune microenvironment, with high-risk patients exhibiting elevated levels of immune cell infiltration. Drug sensitivity analysis revealed negative correlations between FOXP1 and Daporinad, ABT737, and BI.2536, while SIRT2 showed positive correlations with ABT737, BI.2536, and ULK1_4989. Conclusion: We have constructed an angiogenesis-related gene prognostic signature that enriches the prognostic assessment system for AML and provides novel therapeutic directions for this disease.
Prevalence of latent tuberculosis infection determined by interferon-gamma release assays in patients with immune-mediated diseases
Authors: Iglesias Marisol Apaez , Navarro Juan Pablo Sánchez , Madinaveitia Diana Edith Fernández , Marín José Antonio Mata , Angulo Gustavo Barriga , González Salma Triana , Díaz Ana Luz Cano , Martínez Jesús Enrique Gaytán 
Published: October 2024

Abstract

Background Patients with Immune-mediated inflammatory diseases (IMIDs) are at high risk of reactivation of tuberculosis, the risk is determined on the type of drug disease-modifying antirheumatic drugs (DMARDs). The objective of this study was to determine the prevalence of latent tuberculosis infection (LTBI) and associated factors in people who started or who were on DMARDs treatment.Methods A cross-sectional study was conducted in Mexico City from January 2021 to June 2024. People ≥ 18 years old, in the clinic of IMIDs, on mainly biological DMARDs treatment or before it was started. The prevalence of LTBI was determined by gamma interferon release assay (QuantiFERON-TB Gold Plus). Categorical data were expressed as frequencies and percentages, quantitative data in median and interquartile ranges, prevalence of LTBI was presented as percentage. A bivariate analysis was performed with x2 test to identify associated factors. A multivariate analysis was performed using a logistic regression model.Results A total of 304 patients were analyzed, 154 (50.7%) were men, with median age of 53 (IQR 39–61) years. Prevalence of LTBI was 34.2% (95% CI 29.1%-39.7%). The most frequent IMIDs were psoriasis in 123 (40.4%) and, rheumatoid arthritis in 47 (15.4%). The most prescribed biological DMARDs were adalimumab in 132 (43.4%) and secukinumab in 46 (15.1%). After a logistic regression model, prior exposure to contacts diagnosed with tuberculosis OR 4.20 (95% CI 1.74–10.12, p = 0.001) and TST ≥ 5 mm OR 99.3 (95%CI 12.7-773.2) remain statistical significance.Conclusions A high prevalence of LTBI was found in patients with IMIDs treated with biological DMARDs. tumor necrosis factor alpha inhibitors were widely prescribed in these patients. The history of exposure to contacts diagnosed with tuberculosis and TST ≥ 5 mm were associated with positive IGRA for LTBI.
PD-1 Blockade-activated Neoantigen Specific Cellular Therapy For Advanced Relapsed Non Small Cell Lung Cancer
Authors: Qiao Yun , Hui Kaiyuan , Hu Chenxi , Wang Mei , Sun Wen , Liu Liang , Tang Jingjiao , Jiang Xiaodong 
Published: October 2024

Abstract

Background
Due to its strong immunogenicity and tumor specificity, neoplastic antigen has emerged as an immunotherapy target with wide therapeutic prospect and clinical application value. Anti-programmed death-1 (PD-1) antibodies reinvigorate T cell-mediated antitumor immunity .So we conducted single-arm trial to assess the safety and efficacy of PD-1 blockade(Camrelizumab)-activated Neoantigen Specific Cellular Therapy (aNASCT) on advanced relapsed non-small lung cancer( NSCLC)(ClinicalTrials.gov NCT03205930).
Methods
Neoantigenic peptides were designed and manufactured according to the whole-exome sequencing and RNA sequencing of fresh biopsy tissues and peripheral blood as well as bioinformatics analysis.All participants received subcutaneous injection of mature DCS (mDCS) loaded with neoantigens on day 8 and subcutaneous injection of mDC-induced autologous somatic toxic T lymphocytes (CTLS) on day 27 for a period defined as 28 days (4 weeks). Enrolled patients received at least 3 cycles of therapy.The safety and efficacy of the treatment were evaluated by evaluating adverse reactions, progression-free survival (PFS), overall survival (OS).
Results
A total of 13 patients with advanced replased NSCLC were enrolled in this study. All 13 patients received at least 3 cycles of aNASCT treatment, of which 2 patients received at most 12 cycles of treatment. Treatment-related adverse events (AEs) occurred in 4/13 (30.8%)patients with transient fever below 38℃.The objective response rate (ORR) across the 13 enrolled patients was 7 of 13 (53.85%).The disease control rate (DCR) was 8 of 13 (61.54%). The median PFS was 11 months (95% CI 6.1–15.9), and the median OS was 15 months(95% CI 11.5–18.5).
Conclusions
Our findings indicate that aMASCT therapy was safety and immunogenicity of patients with advanced relapsed NSCLC, suggesting its promising potential in cancer immunotherapy.
Risk factors associated with lymph node metastasis in papillary thyroid cancer: a retrospective analysis based on 2428 cases
Authors: Yu Kejie , Wu Xianjiang , Dai Lei , Le Qi , Xie Yue , Wang Yingchun , Zhang Weidong 
Published: October 2024

Abstract

Background
Papillary thyroid cancer (PTC) with lymph node metastasis (LNM) is associated with an increased risk of postoperative recurrence. Understanding the clinical characteristics of PTC patients can help surgeons assess the likelihood of LNM. This study aims to identify risk factors for LNM in PTC patients.
Methods
We retrospectively analyzed clinical data from 2,428 patients diagnosed with PTC who underwent initial thyroid surgery in our single centre. Risk factors related to central lymph node metastasis (CLNM), lateral lymph node metastasis (LLNM), and skip metastasis were investigated by univariate and multivariate binary logistic regression analyses.
Results
Univariate analysis revealed that the following factors were associated with an increased likelihood of CLNM (P < 0.05): male gender, age < 45 years, maximal axial diameter (MAD) > 1 cm, body mass index (BMI) ≥ 28 kg/m² and multifocality. Univariate analysis also revealed that patients with age < 45 years, MAD > 1 cm, BMI ≥ 28 kg/m², multifocality, and CLNM demonstrated a higher propensity for LLNM (P < 0.05). Lower pole tumors were more likely to have CLNM than upper pole tumors, while upper pole tumors were more prone to present LLNM (P < 0.05) and skip metastasis (P < 0.05). Multivariable binary logistic regression analysis identified that gender (odds ratio [OR], 1.732; 95% CI, 2.113–2.577; P < 0.001), age (OR, 1.905; 95% CI, 1.596–2.273; P < 0.001), MAD (OR, 4.639; 95% CI, 3.639–5.913; P < 0.001), and multifocality (OR, 1.860; 95% CI, 1.453–2.381; P < 0.001) were independent risk factors for CLNM and MAD (OR, 5.289; 95% CI, 3.777–7.404; P < 0.001), multifocality (OR, 1.858; 95% CI, 1.248–2.766; P = 0.002), and CLNM (OR, 5.030; 95% CI, 3.347–7.561; P < 0.001) for LLNM.
Conclusion
Despite the overall postoperative recurrence rate in PTC patients is low, identifying risk factors such as male gender, age < 45 years, MAD > 1 cm, multifocality, and CLNM can help predict LNM. In specific cases, selective lymphadenectomy in the central or lateral neck area may be warranted.
Mechanisms of Influence of Migration Processes on the Sustainable Development of Enterprise Economics (Case of Azerbaijan)
Authors: Adli Maryam , Ahmadov Elshan 
Published: October 2024

Abstract

This paper summarises the theoretical foundations and practical provisions on the mechanisms of influence of migration processes on the sustainable development of the enterprise economics (on the case of Azerbaijan). The role of migration processes in the sustainable development of the enterprise economy is emphasised by correlation with both migration and the development of the enterprise economy. The positive role of migrant workers in the economic development of countries is confirmed by determining the benefits of migration and supporting the results obtained with statistical indicators. We made the correlation and regression analysis, to define the link between migration and economic development indicators in Azerbaijan. As a result of these and statistical analysis, recommendations were developed for further expanding opportunities for immigrants to strengthen their impact on the enterprise economy in Azerbaijan.
JEL classification: Q01, M21, J61, O31
Adenosine deaminase family acting on RNA 1 may predict efficacy for intrauterine infection
Authors: Nakamura Keiichiro , Shigeyasu Kunitoshi , Vu Thuy Ha , Maki Jota , Okamoto Kazuhiro , Masuyama Hisashi 
Published: October 2024

Abstract

Intrauterine infection (IUI) is mainly an ascending infection in which vaginal and cervical pathogens ascend to the uterus and can affect the fetus. Until now, there is still no effective diagnostic biomarker for IUI, such as chorioamnionitis (CAM) and funisitis (FUN). Deoxyribonucleic acid (DNA)/Ribonucleic acid (RNA) editing molecules such as apolipoprotein-B mRNA-editing complex (APOBEC) 3 families and Adenosine deaminase family acting on RNA (ADAR)1 were examined in chorioamniotic membranes and umbilical cord of 83 patient samples. Furthermore, the biological functions of ADAR1 induced by ureaplasma parvum were investigated in the human HTR-8/SVneo EVT cell line. ADAR1 had a significantly higher area under the curve (AUC) (0.721 and 0.745) than other APOBEC3s or cytokines in CAM and FUN patients. In vitro, ureaplasma parvum was demonstrated to activate ADAR1 (p = 0.025) and reduce RIG-I, IRF3, IFN-α, and IFN-β expression in EVT cell line (p = 0.005, p = 0.010, p < 0.001, and p = 0.018, respectively). High expression of ADAR1 was strongly associated with CAM and FUN patients (multivariate analyses; p = 0.035 and p = 0.002). ADAR1 could be a potential diagnostic target for IUI, such as CAM and FUN patients.