bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells
Authors: Mazeres Serge , Fereidouni Farzad , Joly Etienne 
Published: June 2017

Background:We wanted to investigate the physical state of biological membranes in live cells under the most physiological conditions possible.Methods:For this we have been using laurdan, C-laurdan or M-laurdan to label a variety of cells, and a biphoton microscope equipped with both a thermostatic chamber and a spectral analyser. We also used a flow cytometer to quantify the 450/530 nm ratio of fluorescence emissions by whole cells.Results:We find that using all the information provided by spectral analysis to perform spectral decomposition dramatically improves the imaging resolution compared to using just two channels, as commonly used to calculate generalized polarisation (GP). Coupled to a new plugin called Fraction Mapper, developed to represent the fraction of light intensity in the first component in a stack of two images, we obtain very clear pictures of both the intra-cellular distribution of the probes, and the polarity of the cellular environments where the lipid probes are localised.Our results lead us to conclude that, in live cells kept at 37°C, laurdan, and M-laurdan to a lesser extent, have a strong tendency to accumulate in the very apolar environment of intra-cytoplasmic lipid droplets, but label the plasma membrane (PM) of mammalian cells ineffectively. On the other hand, C-laurdan labels the PM very quickly and effectively, and does not detectably accumulate in lipid droplets.Conclusions:From using these probes on a variety of mammalian cell lines, as well as on cells fromDrosophilaandDictyostelium discoideum, we conclude that, apart from the lipid droplets, which are very apolar, probes in intracellular membranes reveal a relatively polar and hydrated environment, suggesting a very marked dominance of liquid disordered states. PMs, on the other hand, are much more apolar, suggesting a strong dominance of liquid ordered state, which fits with their high sterol contents.
Reconstruction of the stadium “Ermak” and Jubilee Square in the city of Novocherkassk
Authors: Кибалова Татьяна , Kibalova Tatyana , Тумасов Александр , Tumasov Aleksandr , Петренко Яна , Petrenko Yana 
Published: June 2017

Reconstruction of the stadium “Ermak” in the city of Novocherkassk associated with the preparation for the World Cup in 2018. Analysis of existing buildings detects the variety (diversity) and disharmony in the artistic and compositional (art and composite) decision of the area. The structure of the objects forming the area included buildings of meliorative academy, cinema “Kosmos”, hotel “Novocherkassk” and a 14-storeyed house. The southern part of the square is finished now by the entrance group of premises of stadium which isn’t meeting the requirements of further development of the sporting object.
To complete the ensemble, its connection with the natural environment, selected the architectural and stylistic concept, expressed in the new object. The multifunctional (multipurpose) complex of the ‘Ermak’ stadium including premises of hotel, administration and trade can become such object. This object is a program in the preparation of all stadium for the forthcoming World Cup.
Optimization of Warehouse Processing Technology on the Basis of Economic Criteria
Authors: Пересветов  , Peresvetov Yu. , Вдовин  , Vdovin D. , Качалин  , Kachalin I. 
Published: June 2017

Th e article considers the organization of the technology of warehouse cargo handling, the ABC (Activity based costing) method is researched, which is used to estimate the unit cost of technological operations, the analysis of existing
options for technological operations of processing the cargo fl ow of the warehouse with the allocation of cost objects is given.
Digital manufacturing, industry 4.0, clould computing and thing internet: Brazilian contextualization and reality
Authors: Cardoso Wagner , Azzolini Junior Walther , Bertosse Jéssica Fernanda , Bassi Edson , Ponciano Emanuel Soares 
Published: June 2017

The digital era represents significant changes in the design of IT projects with an emphasis on digital infrastructure, especially in terms of investment and professional qualification, which requires, in Brazil, the creation of specific lines of financing by government development agencies. The creation of demonstration platforms could be an effective initiative to stimulate the dissemination of the concept and the establishment of partnerships between customers and suppliers of new technologies. On the other hand, and particularly for the consumer market, corporations can create new business models and modify their relationships with their consumers, users and even competitors. In fact, today, "Thing Internet" has come to significantly modify the paradigms of perception, production and distribution of the capitalist world. This article discusses, covering and understanding the main reasons for the existence of this gap between theory and practice regarding digital manufacturing and adjacencies, the perspectives of technological innovations in the digital era specifically in Brazil. Its content is the result of a bibliographical review carried out from April to June 2016.
Functional Performance and Balance in the Oldest-Old
Authors: Kafri Michal , Hutzler Yeshayahu , Korsensky Olga , Laufer Yocheved 
Published: June 2017

Background and Purpose:The group of individuals 85 years and over (termed oldest-old) is the fastest-growing population in the Western world. Although daily functional abilities and balance capabilities are known to decrease as an individual grows older, little is known about the balance and functional characteristics of the oldest-old population. The aims of this study were to characterize balance control, functional abilities, and balance self-efficacy in the oldest-old, to test the correlations between these constructs, and to explore differences between fallers and nonfallers in this age group.Methods:Forty-five individuals living in an assisted living facility who ambulated independently participated in the study. The mean age was 90.3 (3.7) years. Function was tested using the Late-Life Function and Disability Instrument (LLFDI). Balance was tested with the mini-Balance Evaluation System Test (mini-BESTest) and the Timed Up and Go (TUG) test. Balance self-efficacy was tested with the Activities-Specific Balance Confidence (ABC) scale.Results:The mean total function LLFDI score was 63.2 (11.4). The mean mini-BESTest score was 69.8% (18.6%) and the mean TUG time was 12.6 (6.9) seconds. The mean ABC score was 80.2% (14.2%). Good correlation (r> 0.7) was observed between the ABC and the function component of the LLFDI, as well as with the lower extremity domains. Correlations between the mini-BESTest scores and the LLFDI were fair to moderate (r's range: 0.38-0.62). Age and ABC scores were significant independent explanators of LLFDI score (P= .0141 andP= .0009, respectively). Fallers and nonfallers differed significantly across all outcome measures scores, except for TUG and for the “Reactive Postural Control” and “Sensory Orientation” domains of the mini-BESTest.Discussion and Conclusions:The results of this study provide normative data regarding the balance and functional abilities of the oldest-old, and indicate a strong association between self-efficacy and function. These results emphasize the importance of incorporating strategies that maintain and improve balance self-efficacy in interventions aimed at enhancing the functional level of this cohort.
Effects of a 6-Week Upper Extremity Low-Volume, High-Intensity Interval Training Program on Oxygen Uptake, Peak Power Output, and Total Exercise Time
Authors: Pinto Nicole , Salassi James W. , Donlin Ayla , Schroeder Jan , Rozenek Ralph 
Published: June 2017

Abstract
Pinto, N, Salassi III, JW, Donlin, A, Schroeder, J, and Rozenek, R. Effects of a 6-week upper extremity low-volume, high-intensity interval training program on oxygen uptake, peak power output, and total exercise time. J Strength Cond Res 33(5): 1295–1304, 2019—The purpose of this study was to compare the effects of upper extremity (UE) high-intensity interval training (HIIT) to UE continuous training (CT) when training at a similar intensity. Twenty participants (mean age = 23 ± 3 years) were randomly assigned to either a HIIT (n = 10) or CT (n = 10) group. Participants completed a graded exercise test (GXT) utilizing arm cranking before and after 6 weeks (2 sessions per week) of UE training. During sessions, HIIT performed 10 repetitions of 60 seconds of work at 92.3 ± 1.0% of the arm HRpeak (%aHRpeak) and 60 seconds of passive recovery (%aHRpeak = 73.0 ± 4.0%) yielding an average training intensity of 82.6 ± 1.5 %aHRpeak. CT exercised for 20 minutes at an average intensity of 81.9 ± 2.2 %aHRpeak. After training, HIIT showed greater improvement in VO2peak compared with CT (Δ = 4.1 ml·min−1·kg−1, 95% confidence interval [CI]: 1.3–6.9 m·min−1·kg−1, p = 0.007). Total exercise time during the posttest GXT was also improved as a result of HIIT (Δ = 1.4 minutes, 95% CI: 0.4–2.3 minutes, p = 0.008). Both groups improved peak power output, but no difference was observed between them (Δ = 3.3 W, 95% CI: −3.3 to 9.9 W, p = 0.305). For a similar time investment, HIIT seemed to improve measures of cardiopulmonary capacity and exercise time to a greater extent than CT and may be a time-efficient alternative for those who incorporate UE aerobic activity into a training program.
CDK4 phosphorylation status and a linked gene expression profile predict sensitivity to palbociclib
Authors: Raspé Eric , Coulonval Katia , Pita Jaime M , Paternot Sabine , Rothé Françoise , Twyffels Laure , Brohée Sylvain , Craciun Ligia , Larsimont Denis , Kruys Véronique , Sandras Flavienne , Salmon Isabelle , Van Laere Steven , Piccart Martine , Ignatiadis Michail , Sotiriou Christos , Roger Pierre P 
Published: June 2017

AbstractCyclin D‐CDK4/6 are the first CDK complexes to be activated in the G1 phase in response to oncogenic pathways. The specific CDK4/6 inhibitor PD0332991 (palbociclib) was recently approved by the FDA and EMA for treatment of advanced ER‐positive breast tumors. Unfortunately, no reliable predictive tools are available for identifying potentially responsive or insensitive tumors. We had shown that the activating T172 phosphorylation of CDK4 is the central rate‐limiting event that initiates the cell cycle decision and signals the presence of active CDK4. Here, we report that the profile of post‐translational modification including T172 phosphorylation of CDK4 differs among breast tumors and associates with their subtypes and risk. A gene expression signature faithfully predicted CDK4 modification profiles in tumors and cell lines. Moreover, in breast cancer cell lines, the CDK4 T172 phosphorylation best correlated with sensitivity to PD0332991. This gene expression signature identifies tumors that are unlikely to respond to CDK4/6 inhibitors and could help to select a subset of patients with HER2‐positive and basal‐like tumors for clinical studies on this class of drugs.
Long-acting PEGylated recombinant human growth hormone (Jintrolong) for children with growth hormone deficiency: phase II and phase III multicenter, randomized studies
Authors: Luo Xiaoping , Hou Ling , Liang Li , Dong Guanping , Shen Shuixian , Zhao Zhuhui , Gong Chun Xiu , Li Yuchuan , Du Min-lian , Su Zhe , Du Hongwei , Yan Chaoying 
Published: June 2017

Objective
We assessed the efficacy and safety of a weekly pegylated human growth hormone (PEG-rhGH) (Jintrolong) vs daily rhGH for children with growth hormone deficiency (GHD).

Design
Phase II and III, multicenter, open-label, randomized controlled trials.

Methods
108 and 343 children with treatment-naive GHD from 6 hospitals in China were enrolled in the phase II and III studies respectively. Patients in the phase II study were randomized 1:1:1 to weekly Jintrolong (0.1 mg/kg/week PEG-rhGH complex), weekly Jintrolong (0.2 mg/kg/week PEG-rhGH complex) or daily rhGH (0.25 mg/kg/week) for 25 weeks. Patients in the phase III study were randomized in a 2:1 ratio to weekly Jintrolong (0.2 mg/kg/week) or daily rhGH (0.25 mg/kg/week) for 25 weeks. The primary endpoint for both studies was height velocity (HV) increase at the end of treatment. Other growth-related parameters, safety and compliance were also monitored.

Results
The phase II study established the preliminary efficacy, safety and recommended dose of Jintrolong PEG-rhGH. In the phase III study, we demonstrated significantly greater HV increases in patients receiving Jintrolong treatment (from 2.26 ± 0.87 cm/year to 13.41 ± 3.72 cm/year) vs daily rhGH (from 2.25 ± 0.82 cm/year to 12.55 ± 2.99 cm/year) at the end of treatment (P < 0.05). Additionally, significantly greater improvement in the height standard deviation scores was associated with Jintrolong throughout the treatment (P < 0.05). Adverse event rates and treatment compliance were comparable between the two groups.

Conclusion
Jintrolong PEG-rhGH at a dose of 0.2 mg/kg/week for 25 weeks is effective and safe for GHD treatment and is non-inferior to daily rhGH.
Enhanced self-efficacy after a self-management programme in pituitary disease: a randomized controlled trial
Authors: Andela Cornelie D , Repping-Wuts Han , Stikkelbroeck Nike M M L , Pronk Mathilde C , Tiemensma Jitske , Hermus Ad R , Kaptein Adrian A , Pereira Alberto M , Kamminga Noelle G A , Biermasz Nienke R 
Published: June 2017

ContextPatients with pituitary disease report impairments in Quality of Life (QoL) despite optimal biomedical care. Until now, the effects of a self-management intervention (SMI) addressing psychological and social issues for these patients and their partners have not been studied.ObjectiveTo examine the effects of a SMI i.e. Patient and Partner Education Programme for Pituitary disease (PPEP-Pituitary).Design and subjectsA multicentre randomized controlled trial included 174 patients with pituitary disease, and 63 partners were allocated to either PPEP-Pituitary or a control group. PPEP-Pituitary included eight weekly sessions (90 min). Self-efficacy, bother and needs for support, illness perceptions, coping and QoL were assessed before the intervention (T0), directly after (T1) and after six months (T2). Mood was assessed before and after each session.ResultsPatients in PPEP-Pituitary reported improved mood after each session (except for session 1). In partners, mood only improved after the last three sessions. Patients reported higher self-efficacy at T1 (P = 0.016) which persisted up to T2 (P = 0.033), and less bother by mood problems directly after PPEP-Pituitary (P = 0.01), but more bother after six months (P = 0.001), although this increase was not different from baseline (P = 0.346). Partners in PPEP-Pituitary reported more vitality (P = 0.008) which persisted up to T2 (P = 0.034). At T2, partners also reported less anxiety and depressive symptoms (P ≤ 0.014).ConclusionThis first study evaluating the effects of a SMI targeting psychosocial issues in patients with pituitary disease and their partners demonstrated promising positive results. Future research should focus on the refinement and implementation of this SMI into clinical practice.
Receiving Providers’ Perceptions on Information Transmission During Interfacility Transfers to General Pediatric Floors
Authors: Rosenthal Jennifer L. , Romano Patrick S. , Kokroko Jolene , Gu Wendi , Okumura Megumi J. 
Published: June 2017

BACKGROUND:Pediatric patients can present to a medical facility and subsequently be transferred to a different hospital for definitive care. Interfacility transfers require a provider handoff across facilities, posing risks that may affect patient outcomes.OBJECTIVES:The goal of this study was to describe the thoroughness of information transmission between providers during interfacility transfers, to describe perceived errors in care at the posttransfer facility, and to identify potential associations between thoroughness of information transmission and perceived errors in care.METHODS:We performed an exploratory prospective cohort study on communication practices and patient outcomes during interfacility transfers to general pediatric floors. Data were collected from provider surveys and chart review. Descriptive statistics were used to summarize survey responses. Logistic regression was used to analyze the association of communication deficits with odds of having a perceived error in care.RESULTS:A total of 633 patient transfers were reviewed; 218 transport command physician surveys and 217 frontline provider surveys were completed. Transport command physicians reported higher proportions of key elements being included in the verbal handoff compared with frontline providers. The written key element transmitted with the lowest frequency was a summary document (65.2%), and 13% of transfers had at least 1 perceived error in care. Transfers with many deficits were associated with higher odds of having a perceived error in care.CONCLUSIONS:Information transmission during pediatric transfers is perceived to be inconsistently complete. Deficits in the verbal and written information transmission are associated with odds of having a perceived error in care.
Assessing the Fragile X Syndrome Newborn Screening Landscape
Authors: Riley Catharine , Wheeler Anne 
Published: June 2017

BACKGROUND:
Fragile X syndrome (FXS) is the most common known inherited form of intellectual disability. Early identification is an important step in linking FXS individuals with appropriate and timely medical and social services. Newborn screening (NBS) is 1 approach that has been used for other conditions to facilitate early identification.

METHODS:
A literature review was conducted to identify issues, barriers, challenges, and approaches to addressing challenges related to NBS for FXS. Search terms included: fragile X syndrome, FMR1, newborn screening, screening, and genetic testing. To supplement the literature review, 9 key informant interviews were conducted. Information gathered through these interviews supplemented what was identified in the literature. Information from both the literature review and supplemental interviews was reviewed by 3 researchers who discussed and came to consensus on thematic areas and categorization of issues.

RESULTS:
The barriers and challenges related to NBS for FXS identified in the literature and by experts and stakeholders are categorized into 5 thematic areas: public health burden, treatment, timing, screening/testing methodologies, and translating results. Summaries of these issues and barriers are provided, along with potential approaches to addressing them.

CONCLUSIONS:
The issues and barriers described in this article highlight limited areas of knowledge that need be addressed to improve our understanding of FXS and the potential benefit of NBS. The landscape of NBS for FXS could be influenced by a series of research findings over time or a larger breakthrough that demonstrates an effective targeted treatment that has to be implemented early in life.
Routine careful histopathological examination should be performed in sleeve gastrectomy specimens
Authors: Vardar Enver , Öztürk Ahmet Mücteba , Comut Erdem , Ersoz Didem , Erkul Zehra , Yıldırım Mehmet 
Published: June 2017

Introduction: The presence of increased ratio of obesity caused a rapid increase of bariatric surgery practice. In this study, our purpose is to clarify the histopathologic findings of the patients who have experienced this type of bariatric surgery and to learn more about the distinguishable features of laparoscopic sleeve gastrectomy (LSG) specimens and to contribute to the related literature.
Methods: A retrospective study was designed with the histopathologic findings from pathology specimens of 109 patients who experienced LSG between April 2014 and May 2016. To collect essential data, we used the database system of our institution that contains all of the selected patients for our study.
Results: Overall, the average age was 36.2 years, and 85 were female while 24 were male. The principal histopathologic features were active chronic gastritis in 9, chronic gastritis in 68, atrophy in 5, and intestinal metaplasia in 8 patients. In two patients, gastrointestinal stromal tumor was found and in another patient, neuroendocrine cell hyperplasia was found. In addition to the dominant histopathologic features including chronic and active chronic gastritis, a small percent of patients had clinically significant pathologic findings in the sleeve gastrectomy specimens and this may have an effect on postoperative management and morbidity.
Conclusion: Considering these results, having histopathologic examination of the sleeve gastrectomy specimens as a standard procedure is strongly recommended.
ATIVIDADE INSETICIDA DE Nicotiana tabacum E Azadirachta indica SOBRE Helicoverpa armigera Hübner,1908 (Lepidoptera:Noctuidae)
Authors: Durán Aguirre Cristhian Eliseo , De Araujo Junior Luis Moreira , Fragoso Débora Ferreira Melo , Ataíde Julielson de Oliveira , Acevedo Ana Clara Thezolin , Buqueroni Sabrina , Pratissoli Dirceu 
Published: June 2017

A mariposa Helicoverpa armigera Hübner,1908 (Lepidoptera:Noctuidae), mais conhecida como lagarta-do-algodoeiro, quando atingi o nível de controle passa a ser uma praga agressiva e de rápido crescimento populacional. O principal método de controle tem sido por meio de inseticidas químicos sintéticos, cujo efeito pode ser comprometido pelo desenvolvimento de populações resistentes da praga. No intuito de propiciar uma alternativa no manejo da praga em questão, objetivou-se avaliar a atividade inseticida de extratos vegetais em lagartas de primeiro instar de H. armigera. Com isto, avaliou-se a toxicidade aguda dos extratos vegetais, pela contagem de lagartas mortas após 72 horas de tratadas. Os extratos vegetais de Nicotiana tabacum e Azadirachta indica apresentaram mortalidade de 11,31 e 16,80% respectivamente, diferindo estatisticamente. Logo, o produto que apresentou maior valor de mortalidade foi o extrato de A. indica. Palavras-chaves: Controle biológico, inseticidas botânicos, mortalidade
VARIABILIDADE GENÉTICA DE CARACTERES DO CRESCIMENTO INICIAL DE FAMÍLIA DE MEIOS-IRMÃOS DO MAMOEIRO
Authors: Da Silva Dayane Gonçalves , Moitinho Alyce Carla Rodrigues , Kuhlcamp Karin Tesch , Moreira Sarah Ola , De Souza Barros Fabíola Lacerda 
Published: June 2017

A cultura de mamoeiro (Carica papaya) necessita de novas cultivares, mais produtivas, adaptadas e que atendam às exigências dos mercados externo e interno. Nesse trabalho foram avaliados caracteres morfoagronômicos em famílias de meios-irmãos da cultivar Rubi Incaper 511, durante a fase vegetativa. O experimento foi conduzido em delineamento de blocos ao acaso, com cinco repetições e nove plantas por parcela. Foram avaliados dezoito famílias de meios-irmãos provenientes da cultivar Rubi Incaper 511 e a própria cultivar, como testemunha. Foi medida a altura de planta, a altura de inserção dos primeiros frutos e diâmetro de caule. Foi calculada a análise de variância, o coeficiente de variação e o coeficiente de determinação genotípico. As médias foram agrupadas pelo método Scott-Knott. Houve diferenças significativas entre os genótipos para todas as variáveis analisadas. Foi possível identificar famílias com menor AP e AF e maior DF, características que atendam ao interesse de produtores de mamoeiro.