bluuebunny/crossref_metadata_2025_split huggingface dataset:
The comparative morphology of the oral cavity glands in captive South African painted dogs (Lycaon pictus pictus) and captive fennec foxes (Vulpes zerda) (Carnivora: Canidae)
Authors: Klećkowska-Nawrot Joanna , Stegmann Krzysztof , Dziech Arkadiusz , Jędrszczyk Gabriela , Jucenco Igor , Barszcz Karolina , Goździewska-Harłajczuk Karolina 
Published: October 2024

Abstract
Background
The African painted dog is classified as a relic canid lineage, inhabiting areas south of the Sahara. The fennec fox is the smallest member of the Canidae family, found in the Arabian Peninsula and northern Sahara.

Methods
The gross anatomy and light microscopic examination of the oral cavity glands were studied in five adult captive South African painted dogs and five adult captive fennec foxes from the Wroclaw Zoological Garden, Poland. In this research, the zygomatic gland, monostomatic sublingual gland, polystomatic sublingual gland, mandibular gland, and parotid gland were examined for their topography, morphometry, histology, and histochemistry using hematoxylin and eosin, azan trichrome, mucicarmine, PAS, AB pH 1.0, AB pH 2.5, AB pH 2.5 PAS, and HID.

Results
We found that the parotid glands were consistently the largest, followed by the mandibular and sublingual glands (both monostomatic and polystomatic). The zygomatic gland was the smallest in both South African painted dogs and fennec foxes. Interestingly, there were noticeable differences in the size, shape, and even composition of the secretory products between the two species. The zygomatic and polystomatic sublingual glands in the South African painted dog and the fennec fox were complex branched tubular. In the South African painted dog, the monostomatic gland was a branched tubular compound gland, while in the fennec fox, it was a branched tubuloalveolar compound gland. The mandibular gland in hunting dogs was a branched tubular compound gland, while in the fennec fox a branched tubuloalveolar compound gland. The parotid gland in the fennec fox was a branched acinar compound gland, whereas in the painted dog was a branched tubuloacinar.

Conclusions
The basic structure of their glands is similar to that of other terrestrial carnivores, indicating a shared evolutionary origin and function. However, differences in the composition of their secretory products can reflect adaptations to their specific diets. This research provides valuable insights for veterinary medicine and underscores the importance of further studies. By analyzing wild canid populations and including a broader range of species with diverse diets, we could gain a deeper understanding of how diet influences salivary gland morphology within the Canidae.
Mechanisms of neural infiltration-mediated tumor metabolic reprogramming impacting immunotherapy efficacy in non-small cell lung cancer
Authors: Zheng Yuanyuan , Li Lifeng , Shen Zhibo , Wang Longhao , Niu Xiaoyu , Wei Yujie , Sun Shilong , Zhao Jie 
Published: October 2024

Abstract
Background
Current evidence underlines the active role of neural infiltration and axonogenesis within the tumor microenvironment (TME), with implications for tumor progression. Infiltrating nerves stimulate tumor growth and dissemination by secreting neurotransmitters, whereas tumor cells influence nerve growth and differentiation through complex interactions, promoting tumor progression. However, the role of neural infiltration in the progression of non-small cell lung cancer (NSCLC) remains unclear.

Methods
This study employs the techniques of immunohistochemistry, immunofluorescence, RNA sequencing, molecular biology experiments, and a murine orthotopic lung cancer model to deeply analyze the specific mechanisms behind the differential efficacy of NSCLC immunotherapy from the perspectives of neuro-tumor signal transduction, tumor metabolism, and tumor immunity.

Results
This study demonstrates that nerve growth factor (NGF) drives neural infiltration in NSCLC, and 5-hydroxytryptamine (5-HT), which is secreted by nerves, is significantly elevated in tumors with extensive neural infiltration. Transcriptome sequencing revealed that 5-HT enhanced glycolysis in NSCLC cells. Pathway analysis indicated that 5-HT activated the PI3K/Akt/mTOR pathway, promoting tumor metabolic reprogramming. This reprogramming exacerbated immunosuppression in the TME. Neutralizing 5-HT-mediated metabolic reprogramming in tumor immunity enhanced the efficacy of PD-1 monoclonal antibody treatment in mice.

Conclusions
The findings of this study provide a novel perspective on the crosstalk between nerves and lung cancer cells and provide insights into further investigations into the role of nerve infiltration in NSCLC progression.
Validation of the Mirai model for predicting breast cancer risk in Mexican women
Authors: Avendano Daly , Marino Maria Adele , Bosques-Palomo Beatriz A. , Dávila-Zablah Yesika , Zapata Pedro , Avalos-Montes Pablo J. , Armengol-García Cecilio , Sofia Carmelo , Garza-Montemayor Margarita , Pinker Katja , Cardona-Huerta Servando , Tamez-Peña José 
Published: October 2024

Abstract
Objectives
To validate the performance of Mirai, a mammography-based deep learning model, in predicting breast cancer risk over a 1–5-year period in Mexican women.

Methods
This retrospective single-center study included mammograms in Mexican women who underwent screening mammography between January 2014 and December 2016. For women with consecutive mammograms during the study period, only the initial mammogram was included. Pathology and imaging follow-up served as the reference standard. Model performance in the entire dataset was evaluated, including the concordance index (C-Index) and area under the receiver operating characteristic curve (AUC). Mirai’s performance in terms of AUC was also evaluated between mammography systems (Hologic versus IMS). Clinical utility was evaluated by determining a cutoff point for Mirai’s continuous risk index based on identifying the top 10% of patients in the high-risk category.

Results
Of 3110 patients (median age 52.6 years ± 8.9), throughout the 5-year follow-up period, 3034 patients remained cancer-free, while 76 patients developed breast cancer. Mirai achieved a C-index of 0.63 (95% CI: 0.6–0.7) for the entire dataset. Mirai achieved a higher mean C-index in the Hologic subgroup (0.63 [95% CI: 0.5–0.7]) versus the IMS subgroup (0.55 [95% CI: 0.4–0.7]). With a Mirai index score > 0.029 (10% threshold) to identify high-risk individuals, the study revealed that individuals in the high-risk group had nearly three times the risk of developing breast cancer compared to those in the low-risk group.

Conclusions
Mirai has a moderate performance in predicting future breast cancer among Mexican women.

Critical relevance statement
Prospective efforts should refine and apply the Mirai model, especially to minority populations and women aged between 30 and 40 years who are currently not targeted for routine screening.

Key Points

The applicability of AI models to non-White, minority populations remains understudied.

The Mirai model is linked to future cancer events in Mexican women.

Further research is needed to enhance model performance and establish usage guidelines.

Graphical Abstract
Exploring Carolingian and Romanesque cycles: a study of the detached wall paintings from the Church of St. Johann in Müstair
Authors: Lombardo Tiziana , Caroselli Marta , Martinucci Camilla , Hildbrand Erwin , Moretti Patrizia , Cassitti Patrick 
Published: October 2024

Abstract
Twenty-six fragment of wall paintings detached from the upper register of the Carolingian and Romanesque cycles of the church of St. John in Müstair (Switzerland) have been part of the Swiss National Museum collection since the beginning of the twentieth century. As some of these objects were in a critical state of conservation, a research project was launched to assess and document their conditions, and to gain knowledge about painting materials and execution techniques, in view of planning a conservation project. In a first phase, non-destructive analyses were carried out, using handheld XRF and portable FTIR spectroscopy; followed by the targeted collection of samples for detailed analysis in the laboratory. In parallel, similar investigations were carried out on the imprints left on the walls of the church’s attic. The results allowed the identification of the pigment palette and the execution technique. Besides earth pigments such as ochres, a lime-based white, probably bianco di San Giovanni, and limited amounts of lapis lazuli were used in both painting cycles. For the Carolingian cycle, Egyptian blue and mixtures of several lead-based pigments were also identified. In several instances, the deterioration of lead-based pigments resulted in shades of grey to black severely modifying the original appearance of the wall paintings. Additionally, both cycles have suffered from pigment alteration due to fire. The obtained results, together with iconographic considerations, allowed for an extended interpretation of possible original colour palette.

Graphical Abstract
Methods of grout quality measurement in borehole exchangers for heat pumps and their rehabilitation
Authors: Nakládal Petr , Procházka Martin , Goliáš Viktor , Hrdá Jaromíra 
Published: October 2024

AbstractMethods and instrumentation for measuring grout quality in heat pump boreholes, including the measurement of groundwater flow through boreholes outside partly grouted borehole exchanger pipes, have been developed in the Czech Republic. A Semtex charge has also been developed to repair rock massifs, which reliably disconnects borehole exchanger pipes without severely harming the surrounding rock environment or buildings. The resulting hole can then be used for regrouting, thus preventing undesirable vertical water flow through the borehole.
Oropharyngeal swab sampling for PRRSV detection in large-scale pig farms: a convenient and reliable method for mass sampling
Authors: Fan Mingyu , Li Yang , Hu Zhiqiang , Bian Lujie , Wu Weisheng , Liu Wei , Li Meng , wang Xinglong , Ren Jing , Wu Lili , Li Xiaowen 
Published: October 2024

Abstract
Background
Porcine reproductive and respiratory syndrome virus (PRRSV) has significant productivity and economic impacts in swine herds. Accurately determining the PRRSV status at the herd level is crucial for producers and veterinarians to implement strategies to control and eliminate the virus from infected herds. This study collected oropharyngeal swabs (OSs), nasal swabs (NSs), oral fluid swabs (OFs), rectal swabs (RSs), and serum samples continuously from PRRSV challenged pigs under experimental conditions and growing pigs under field conditions. Additionally, OSs and serum samples were collected from individual sows from 50 large-scale breeding farms, and the collection of OSs does not require the sows to be restrained. Ct values of PRRSV were detected in all samples using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR).

Results
In PRRSV challenged pigs, OSs showed a higher PRRSV-positive rate until the end of the observation period. The Ct values of OSs were significantly lower than those of NSs, OFs, and RSs at 2, 8, 12, 14 and 20 days post-challenge (DPC) (P < 0.05). For growing pigs, the positivity rate of PRRSV in OSs was higher than that in other sample types at 30, 70, and 110 days of age. In sows, 24,718 OSs and 6259 serum samples were collected, with PRRSV-positive rate in OSs (9.4%) being significantly higher than in serum (4.1%) (P < 0.05). However, the Ct values of PRRSV RNA in serum were significantly lower than those in OSs (P < 0.001).

Conclusions
The OSs sample type yielded higher PRRSV-positive rates for longer periods compared to NSs, RSs, OFs and serum samples for PRRSV detection in infected pigs. Therefore, OSs has a good potential to be a convenient, practical, and reliable sample type for implementing mass sampling and testing of PRRSV in large-scale pig farms.
‘Resettlement is worthwhile for our children’s future’: reflections from the Stateless Rohingya refugees in Malaysia
Authors: Martuscelli Patrícia Nabuco , Ahmed Bayes , Sammonds Peter 
Published: October 2024

AbstractResettlement to a third country offers a durable solution for refugees who cannot integrate into their asylum or transit countries. While most studies focus on the experiences of refugees after their arrival in resettlement countries or on the political aspects of resettlement, fewer studies explore refugees’ perspectives prior to resettlement. This paper examines how Rohingyas in Malaysia view their resettlement opportunities. We collected qualitative micronarratives from 56 adult Rohingyas living in Malaysia between March and September 2019, to explore the complexities of their decision-making processes. The findings indicate that most Rohingyas aspire to resettle in another country to gain legal status or citizenship, providing a better future for their children, including access to education and healthcare. However, preferences among Rohingyas vary: while some are eager to resettle, others express a strong desire to return to their home country in Myanmar. Still, others wish to reunite with family in Myanmar or Bangladesh, and a significant minority are reluctant to resettle in non-Islamic countries, reflecting broader concerns about cultural and religious compatibility. This study highlights the varied resettlement preferences among Rohingyas, underscoring the need for resettlement policies to consider these diverse priorities to enhance the effectiveness of resettlement programs.
Harnessing machine learning for sustainable futures: advancements in renewable energy and climate change mitigation
Authors: Ukoba Kingsley , Onisuru Oluwatayo Racheal , Jen Tien-Chien 
Published: October 2024

Abstract
Background
Renewable energy and climate change are vital aspects of humanity. Energy is needed to sustain life on Earth. The exploration and utilisation of traditional fossil-based energy has led to global warming. The exploration and use of fossil-based energy have significantly contributed to global warming, making the shift to renewable energy crucial for mitigating climate change. Renewable energies offer a sustainable alternative that does not harm the environment. This review aims to examine the role of machine learning (ML) in optimising renewable energy systems and enhancing climate change mitigation strategies, addressing both opportunities and challenges in this evolving field. The vital significance of renewable energy and measures to circumvent climate change in modern civilisation is first contextualised in the review. It draws attention to the difficulties encountered in these fields and describes the exciting potential of ML to solve them. Important discoveries highlight how ML can improve renewable energy technology scalability, dependability and efficiency while enabling more precise climate change projections and practical mitigation strategies. Simultaneously, issues including ethical considerations, interpretability of models and data quality demand attention.

Method
This review conducted a systematic literature analysis on the application of ML in renewable energy and climate change mitigation. It involved a comprehensive search, selection, and analysis of recent studies, focusing on ML’s role in energy forecasting, predictive maintenance, and climate modelling. The review synthesised key developments, challenges, and future directions, emphasising the need for ongoing transdisciplinary research to fully realise ML’s potential in advancing sustainable energy solutions.

Result
The review found that machine learning significantly enhances renewable energy system efficiency, scalability, and climate change mitigation through improved forecasting, predictive maintenance, and climate modelling. However, challenges like ethical concerns, model interpretability, and data quality persist. Ongoing research is essential to fully leverage ML’s potential in these areas.

Short conclusion
The paper highlights how machine learning can be used to revolutionise the energy and climate change mitigation industries for sustainable futures. It promotes ongoing transdisciplinary research and innovation to fully realise ML’s synergistic potential and tackle urgent global issues. In the end, the review advances our knowledge of how to use ML to hasten the transition to a future that is more robust and sustainable.
Profiling hippocampal expression of long non-coding RNA GM12371 in a rat model of vascular dementia
Authors: Hooshmandi Etrat , Akbari Somayeh , Gharbi Negin , Ghobadi Mojtaba , Shahrokhabadi Ava , Salehi Mohammad Saied , Afshari Afsoon , Haghani Masoud , Bayat Mahnaz 
Published: October 2024

Abstract
Background
Dementia, characterized by synaptic dysfunction and memory loss, presents a significant challenge in medical research. Long non-coding RNAs (lncRNAs), known for their crucial roles in regulating gene expression, represent a gap in knowledge regarding their specific involvement in synaptic structure, transmission, and plasticity. This study aims to investigate the dynamic changes in hippocampal lncRNA GM12371 expression in response to acute and chronic hypoperfusion in the rat brain using the 2VO model.

Methods
The 2VO model was induced by permanently occluding the common carotid arteries. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate hippocampal lncRNA GM12371 expression at both acute (day 4) and chronic (day 28) stages post-2VO surgery. Comparative analysis was conducted with a sham-operated group.

Results
A notable reduction in GM12371 expression was observed in the rat hippocampus on day 4 following 2VO surgery compared to the sham group (P < 0.05). However, there was no significant difference in GM12371 expression between the sham and 2VO-28 groups.

Conclusion
These findings suggest that the downregulation of GM12371 is a response to acute hippocampal blood flow reduction but tends to normalize during the chronic phase of cerebral hypoperfusion. This dynamic regulation of GM12371 expression may be significant in the adaptive response to vascular dementia. Further clinical studies are warranted to investigate potential peripheral alterations in GM12371 expression in individuals with dementia.
Assessment of IV albumin and ringer lactate on the acute oral toxicity of acetylsalicylic acid in albino rats
Authors: Harb Inas , Medhat Engy , Samir Mai , Abdel Fattah Shereen , Badawy Hend Ahmed Abdallah , Gamal Sarah Mohamoud , Ateyya Hayam 
Published: October 2024

Abstract
Background
Despite the frequent inclusion of fluid therapy in the treatment of many conditions, there are limited studies available to provide an evidence-based specific recommendation for fluid therapy in acute drug toxicity. Salicylate toxicity is considered one of the common clinical problems. It is commonly associated with fatal complications and even can lead to death. The study was designed to investigate the effects of various IV fluid types as isotonic saline (NaCl 0.9%), Ringer lactate (RL), and albumin and their impact on acetyl salicylic acid (ASA) toxicity outcome in a rat model of acute salicylate toxicity.
Sixty male Albino rats were divided into 10 groups of 6 rats each. The first four groups were the control, saline, RL, and albumin groups. The fifth group received two doses of ASA solution orally, and the next five groups were treated with IV fluids as follows: saline-ASA, RL-ASA, albumin-ASA, RL + albumin-ASA, and saline + albumin-ASA. Upon completion of the study, spirometry, arterial blood gas analysis (ABG), and serum liver and kidney function tests were done on all groups. Furthermore, quantitative real-time polymerase chain reaction (PCR) was used to assess interleukin-6 (IL6), nuclear factor kappa beta (NF-kβ), and beta-actin mRNA gene expression of histopathology and immunohistochemistry assessments were also performed on liver and kidney tissues.

Results
The results revealed the ASA group showed marked deterioration across all the investigated parameters. The groups that received saline and RL showed improvements in the following: respiratory rates, ABG, liver and kidney function, and histopathological findings.
The RL + albumin group did not show any improvements. The albumin group and the saline + albumin group showed variable responses, ranging from mild improvement to no improvement.

Conclusions
The saline and RL groups showed positive results; however, the RL + albumin group showed the worst outcomes. The inclusion of albumin did not appear to provide any extra benefits and produced varying results.
Unnoticed freshwater leech as a hidden cause for recurring epistaxis: a case series and review of literature conducted at Tertiary Care Hospital Sikkim, North East India
Authors: Rai C. S. , Lepcha Pema Seden , Bhutia Tenzing Jigmee , Rai Sachika 
Published: October 2024

Abstract
Background
While leeches as intranasal foreign bodies are not frequently encountered, they can be one of the causes of epistaxis. The aim of the study is to raise awareness among healthcare professionals about the possibility of patients from leech endemic regions presenting with epistaxis.

Case presentation
We present a series of epistaxis cases due to intranasal leech infestation. In our series of 10 cases, there were 7 male and 3 female patients, aged between 2 and 53 years old. The duration of the complaint ranged from 1 to 5 weeks. All patients experienced with blood-stain nasal discharge or frank nasal bleeding at some point and most of them have a history of leech infestation from spring water as a local source. This article presents our approach to managing nasal leech infestation as epistaxis and outlines the precautionary measures taken for the retrieval of this living foreign body.

Conclusion
Details of the history must be taken before concluding the diagnosis. Maintain a high index of suspicion of leech infestation as a hidden cause of epistaxis especially in patients coming from endemic areas.
The quality of the electrodiagnostic referral system and the electrodiagnostic patterns in a sample of Egyptian patients
Authors: Nassif Mohamed Elsayed , Saba Emmanuel Kamal Aziz , El-Tawab Sarah Sayed , Elemary Wafaa Samir , Abdelnaby Hoda Mohamed , Khalil Naylan Fayez Wahba , Ibrahiem Aya Hanafy , Elsakka Ahmed Mosaad Mohamed Arafa , Elshatby Nehad Mohamed 
Published: October 2024

Abstract

Background
Electrodiagnosis is a localizing, diagnostic, and prognostic tool for individuals with suspected neuromuscular diseases. In addition to detailed history taking and thorough clinical examination, correct execution and interpretation of these tests can provide vital insights into the underlying neuromuscular disorders and frequently guide the need for proper management decision. The request for an electrodiagnosis examination should include brief clinical information and a provisional clinical diagnosis; this enhances the efficient easy performance of the test. The current study aims to detect the pattern of neuromuscular diseases in the Alexandria Main University Hospital, Egypt, and to evaluate the quality of the referral system to the electrodiagnosis unit.

Results
The study included 371 patients, out of which, 65% were referred from the Governmental Specialized Medical Councils and the Neurosurgery department. Although sensory complaints were the main reason for referral, yet 28.8% of patients did not show any electrodiagnosis abnormality. A compatible referral note led to shorter test times and fewer electromyography needle insertions.

Conclusion
Disorders of the peripheral nerves are the most frequent neuromuscular diseases referred to the electrodiagnosis unit with carpal tunnel syndrome being the most common disorder. A compatible referral letter is necessary when requesting an electrodiagnosis test, to save the physician time and focus on targeted procedures.