bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obstetric Outcome and its Relationship with Mode of Delivery in Preterm Labour- A Study of 86 Patients in Combined Military Hospital, Dhaka
Authors: Asma Umme Habiba , Juty Sabikun Nahar , Snigdha Rezwana Sharmin 
Published: October 2024

Background: Caesarean section in preterm pregnancy may add further pressure to the already existing poorly developed facilities for intensive perinatal care in low resource countries. The objective of the study was to determine the feto-maternal outcome and its relationship with mode of delivery in preterm labour.
Methods: This was a descriptive type of cross-sectional study conducted at Department of Obstetrics and Gynecology, Combined Military Hospital (CMH), Dhaka, during March to August 20l8. The study population was total eighty-six patients having 28 to 36 weeks of gestation who were admitted in the above department and were in labour. Data were edited and analyzed by using computer-based software, statistical package for social sciences (SPSS) version 20.0.
Results: Majority of the patients belonged to 26-30 years age group and were from low socioeconomic family (71.4 to 72.7) % and had below average nutritional status (88.1 to 93.2) %. The mean gestational age was 31 weeks and 35 weeks. Nulliparous (77.3 to 85.7) % and patients taking irregular antenatal check-up (69.0 to 72.8) % were predominant. About (59.1 to 66.7) % patients had vaginal delivery and (33.3 to 40.9) % patients had undergone caesarean section. Maternal morbidity was more in caesarean section (37.5%) than vaginal delivery (33.3%). Live birth babies were found 100% in caesarean section and 94.4% in vaginal delivery and among them 95.7% were from group I and 97.6% were from group ll. Perinatal mortality was higher in vaginal delivery than caesarean section (7.40% vs 2.7%) and also higher in group I than group ll (8.3% vs 2.3%). But late perinatal complications were more in caesarean section (47.2%) than vaginal delivery (45.1%) baby, and majority (60%) of them were from group l.
Conclusion: Preterm pregnancy when leads to an early delivery, brings a grave consequence for both the mother and foetus. After birth, the premature newborn is at risk for complications related to incomplete development of its organ system. Caesarean section delivery in preterm labour improves foetal outcome to some extent, but not significantly, and leads the mother to more maternal morbidity. Perinatal mortality and morbidity is also inversely related to the gestational age at the time of delivery, in preterm labour - more the gestational age, less the perinatal mortality and morbidity.
Bangladesh Armed Forces Med J Vol 57 No (1) June 2024, pp 43-49
Spot Urine Sodium to Potassium Ratio as a Tool to Assess Severity and Mortality among Patients with Decompensated Cirrhosis having Ascites
Authors: Islam Safikul , Azam Mohammad Golam , Mustafa Md Abu Sayeed , Sajjad Sarker Mohammed , Indrajit Kumar Datta  , Rowshon A H M , Mahmud Arif 
Published: October 2024

Background: Decompensated cirrhosis of liver is considered as a systemic disease affecting the functions of several other organs. Renal function is an independent prognostic factor for patients with decompensated cirrhosis, but assessing renal function through glomerular filtration rate are not convenient, especially for routine use. Previous study found that spot urinary sodium to potassium ratio (UNa/K) was associated with renal dysfunction which influences the severity and outcome in decompensated cirrhosis of liver patients having ascites.
Aims and objectives: The present study was aimed to determine the relation of the ratio of sodium to potassium in randomly collected urine samples with severity of disease and mortality in decompensated cirrhosis having ascites.
Materials and Methods: This longitudinal study was conducted at the Department of Gastrointestinal, Hepatobiliary and Pancreatic Disorders, BIRDEM General Hospital, Shahbagh, Dhaka, Bangladesh, from July, 2019 to August, 202l. A total of 150 patients with a confirmed diagnosis of decompensated cirrhosis with ascites were enrolled in this study. A detailed history and thorough clinical examination were carried out in each patient, along with relevant investigations. Data collection was done through a structured questionnaire. Data were analyzed using the statistical software SPSS 23.
Results: Age of the patients was 59.0±12.91 (mean±SD) years, male predominance was observed (52%). The UNa/K ratio was 4.24±3.25 (mean±SD) with a range of 0.42 to 18.46. Diagnostic accuracy of UNa/K ratio in the detection of severity and mortality was estimated by the receiver operating characteristic (ROC) curve. The AUC of UNa/K ratio was 0.608 and 0.640 for severity and mortality respectively. Sensitivity, specificity, PPV and NPV at cut-off 2.55 were 50.0, 66.0, 42.4 and 72.5; at 2.65 were 54.0, 66.0, 44.3, and 74.2; at 2.87 were 58.0, 62.0, 43.3, and 74.7; at 3.21 were 58.0, 58.0, 40.8, and 73.4 respectively for severity score (MELD). Patients with UNa+/K+ less than 2.87 or equal, had a significantly higher MELD score category (p= 0.02). At 3 months follow-up, 24.7% mortality was observed. Sensitivity, specificity, PPV and NPV at cut-off 1.62 were 51.4, 85.8, 54.3 and 84.3; at 1.79 were 54.1, 79.7, 46.5 and 84.1; at 1.83 were 59.5, 77.0, 45.8 and 85.3; at 2.87 were 58.0, 62.0, 43.3, and 74.7 respectively for mortality. The UNa/K ratio was statistically low among the patients who didn’t survive (p<.05).
Conclusion: This study revealed that decreased ratio of spot urinary sodium to potassium was associated with the severity and mortality among decompensated cirrhosis of liver patients with ascites.
Bangladesh Crit Care J September 2024; 12 (2): 118-124
Evaluation of the Safety and Effectiveness of Ultrasound-guided Internal Jugular Vein Catheterization in Critically Ill Patients
Authors: Sultana Rumana , Khan Moffijul Haque , Morshed Md Mahabub , Asrafuzzaman Mohammad 
Published: October 2024

Background:Central venous catheterization is a frequently performed procedure in intensive care units (ICU) for diagnostic and therapeutic purpose. As an invasive procedure it carries some risk and should be performed with few attempts. Traditionally this procedure is performed blindly by considering body surface landmark, but this procedure can be done with the help of ultrasound machine as an alternative of landmark procedure.
Objectives: Evaluation of the safety and effectiveness of USG-guided internal jugular vein(IJV) catheterization in critically ill patients.
Methods: This prospective observational study was conducted in the ICU of Dhaka Medical College Hospital, from May 2017 to October 2018. Patients scheduled for central venous catheterization via the IJV were included based on selection criteria and randomly allocated into two groups of 50 patients each using card sampling. Group A received ultrasound-guided catheterization, while the landmark technique was used in Group B.
Results: The analysis revealed that in the ultrasound group 49 out of 50 (98.0%) patients were successfully catheterized while the landmark method was successful in 45 out of 50 (90.0%) patients. Successful catheterization by first attempt was possible in 29 patients of group A, where as it was 5 in group B. The average number of attempts for successful catheterization in Group A was 1.7 (SD=0.2) and in the landmark group it was 2.8 (SD=0.1). On the average, 4.9 minutes (SD=1.3) were needed for catheterization in ultrasound group. The time was significantly increased in the landmark group 11.4 (SD=5.8). Total number of complication was 2 in Group A and it was 8 in Group B. After considered all the above parameter, by using four points safety and effectiveness rating scale, safety and effectiveness mean score was 10.3 for Group A and 8.2 for Group B.
Conclusion: Two-dimensional ultrasound offers improved safety and quality when compared with an anatomical landmark technique for IJV catheterization.
Bangladesh Crit Care J September 2024; 12 (2): 96-104
Efektifitas Model Pembelajaran Problem Based Learning Dalam Meningkatkat Keterampilan Berpikir Kritis Siswa Sekolah Dasar
Authors: Komariah Iis , Mutakarikah Mutakarikah , Widati Retno , Pribadi Reksa Adya 
Published: October 2024

Abstract: This research discusses the effectiveness of the Problem Based Learning model in improving students’ critical thinking skills in elementary schools. The method used is literature study, this method is used to collect and analyze the results of the latest research on the use of Problem Based Learning in elementary schools. A thorough analysis of findings from various sources shows that Problem Based Learning can help students develop critical thinking skills through an approach that emphasizes problem solving and cooperation between students. The implication of this research provide useful insights for educators and future educational development.Keywords : Problem Based Learning, critical thinking, elementary schoolAbstrak: Penelitian ini membahas mengenai efektivitas model pembelajaran Problem Based Learning dalam meningkatkan kemampuan berpikir kritis peserta didik di sekolah dasar. Adapun metode yang digunakan adalah studi literatur, metode tersebut digunakan untuk mengumpulkan dan menganalisis hasil peelitian terbaru tentang penggunaan Problem Based Learning di sekolah dasar. Analisis menyeluruh terhadap temuan dari berbagai sumber menunjukkan bahwa Probelm Based Learning dapat membantu peserta didik mengembangkan kemampuan berpikir kritis melalui pendekatan yang menekannkan pada pemecahan masalah dan kerja sama antar peserta didik. Adapun implikasi dari penelitian ini memberikan wawasan yang berguna bagi pendidik dan pengembangan pendidikan di masa depan.Kata kunci : Probelm Based Learning, berpikir kritis, sekolah dasar
Pengaruh Game Online Mobile Legend Terhadap Kedisiplinan Anak Dalam Bersikap diDusun Serumbung Batu Mandi Desa Lembar Selatan Kec. Lembar Kab. Lombok Barat
Authors: Susilawati Hety , Suharyani Suharyani 
Published: October 2024

Abstract: Researchers found that the majority of children in the Serumbung Batu Hamlet area already have one cellphone, already know how to use it, of course the application they will access is game online which is currently trending, namely mobile legends, many of them are addicted to game online mobile legends, as a result of addiction, children become less likely to interact with friends. The aim of this research is to analyze the influence Game Online Mobile Legends Regarding Children's Discipline in Behaving in Serumbung Batu Mandi Hamlet, South Sheet Village, District. District Sheet West Lombok. This research method uses a quantitative approach with a descriptive type, the population in this study is 222 children, 25 children as samples, data collection techniques using questionnaires, observation and interviews, data analysis techniques using simple linear regression formulas processed using Excel and SPPS 26. Results of research conducted in Serumbung Batu Mandi Hamlet, South Sheet Village, District. District Sheet West Lombok, there is influence game online mobile legend towards children's discipline in behaving, it can be concluded that there is a variable influence game online mobile legend (X) of the child's discipline variable in behavior (Y) is 56% and the remaining 44% is influenced by other factors.Keywords: Mobile Legend Online Game, Discipline in BehaviorAbstrak: Peneliti menemukan bahwa sebagian besar anak-anak diwilayah Dusun Serumbung Batu, sudah memiliki handphone, sudah tahu bagaimana cara menggunakannya, tentu aplikasi yang akan mereka akses ialah game online yang saat ini sedang trend yaitu mobile legends, banyak dari mereka kecanduan dengan game online mobile legends, akibat dari kecanduan anak-anak menjadi kurang dalam berinteraksi dengan teman Tujuan dari penelitian ini untuk menganalisis Pengaruh Game Online Mobile Legends Terhadap Kedisiplinan Anak Dalam Bersikap di Dusun Serumbung Batu Mandi Desa Lembar Selatan Kec. Lembar Kab. Lombok Barat. Metode penelitian ini menggunakan pendekatan kuantitatif dengan jenis deskriftif, jumlah populasi dalam penelitian ini adalah sebanyak 222 orang anak, 25 orang anak sebagai sampel, teknik pengumpulan data menggunakan angket, obeservasi, dan wawancara, teknik analisis data menggunakan rumus regresi linier sederhana diolah menggunakan Excel dan SPPS 26. Hasil penelitian yang dilakukan di Dusun Serumbung Batu Mandi Desa Lembar Selatan Kec. Lembar Kab. Lombok Barat, terdapat pengaruh game online mobile legend terhadap kesisplinan anak dalam bersikap, dapat disimpulkan bahwa ada pengaruh variabel game online mobile legend (X) terhadap variabel kedisiplinan anak dalam bersikap (Y) sebesar 56% dan selebihnya 44% dipengaruhi oleh faktor lain.Kata Kunci: Game Online Mobile Legend, Kedisiplinan Dalam Bersikap
Accurate and Objective Lecturer Appraisal System: Implementation of the LOPCOW Method
Authors: Sumanto Sumanto , Radiyah Ummu , Supriyatna Adi , Pujiastuti Lise , Yani Ahmad , Marita Lita Sari 
Published: October 2024

This research proposes the use of the Logarithmic Precursor Chain-Driven Objective Weighting (LOPCOW) method to evaluate the best lecturers in universities. The LOPCOW method ensures that the assessment covers all aspects of lecturer quality and performance, including education, research, community services, discipline, commitment, cooperation skills, and innovation. The evaluation of lecturers using the scores and ratings provided showed that CDE lecturers were the best, with the highest score of 0.715. CDE lecturers showed high consistency in all aspects assessed, especially in education, research, and community service. This was followed by MNO lecturers (0.676), STU lecturers (0.668), XYZ lecturers (0.637), and AFI lecturers (0.627). In conclusion, highly ranked lecturers showed strong dedication to the Tridharma of higher education, with consistent performance and a positive impact on the academic community and the general public. Future research should focus on developing strategies to improve lecturers' teaching quality by applying new educational technologies and evaluating their impact on student learning.
Pembentukan Kader Kesehatan Sebagai Upaya Perbaikan Status Gizi Siswa Bagian Dari Implementasi Health Promoting Schools
Authors: Ashari Chica Riska , Linda Ony , Badzlina Fildzah 
Published: October 2024

ABSTRAK
Remaja merupakan masa peralihan menuju kedewasaan. Masa peralihan tersebut membutuhkan asupan gizi agar dapat berlangsung secara optimal. Adanya pola makan remaja yang tidak baik yang dapat memengaruhi status gizi remaja. Tujuan kegiatan ini yaitu membentuk kader muda gizi dengan memberikan pengetahuan dan pelatihan kepada remaja melalui metode penyuluhan. Hasil kegiatan ini diketahui bahwa kegiatan ini diikuti oleh 218 siswa-siswi. Uji Satistik Paired t-Test diperoleh hasil p value yaitu 0,000 (p value < 0,05) yang artinya bahwa terdapat perbedaan antara pengetahuan peserta sebelum dan sesudah diberikan penyuluhan. Pengetahuan peserta mengalami peningkatan dari sebelum diberikan penyuluhan (pretest) yaitu dengan rata-rata nilai 67 poin dengan nilai minimal yaitu 27 dan nilai maksimal yaitu 87 meningkat menjadi rata-rata 87 poin setelah diberikan penyuluhan (postest) dengan nilai minimal 60 dan nilai maksimal 100. Hal ini menunjukkan bahwa metode penyuluhan yang dilakukan dapat meningkatkan pengetahuan peserta, sehingga peserta dapat menjawab soal pre dan postest dengan baik. Peningkatan pengetahuan ini juga berarti bahwa kegiatan ini berhasil. Diharapkan dengan terbentuknya kader muda gizi ini, mereka dapat berdaya sendiri serta dapat membantu teman sebaya, keluarga mereka ataupun orang lain dalam menjaga status kesehatan dan gizi mereka agar tetap normal.
Kata kunci: Edukasi, Kader Gizi, Penyuluhan, Remaja, Status Gizi

ABSTRACT
Adolescence is a period of transition to maturity. This transition period requires nutritional intake so that it can take place optimally. There are unhealthy eating patterns in adolescents that can affect the nutritional status of adolescents. The purpose of this activity is to form young nutrition cadres by providing knowledge and training to youth through counseling methods. The results of this activity are known that this activity was attended by 218 students. The Paired t-Test statistical test obtained a p value of 0.000 (p value <0.05), which means that there is a difference between the participants' knowledge before and after being given counseling. Participants' knowledge has increased from before being given counseling (pretest), with an average score of 67 points with a minimum score of 27 and a maximum score of 87 increasing to an average of 87 points after being given counseling (posttest) with a minimum score of 60 and a maximum score of 100 This shows that the counseling method used can increase the knowledge of the participants, so that the participants can answer the pre and posttest questions well. This increase in knowledge also means that the activity is successful. It is hoped that with the formation of these young nutrition cadres, they will be self-sufficient and can help their peers, their families or other people in maintaining their health and nutritional status so that they remain normal.
Keywords: Education, Nutrition Cadres, Counseling, Adolescents, Nutritional Status
CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger
Authors: Yuan Guoliang , Deng Shuang , Czajka Jeffrey J. , Dai Ziyu , Hofstad Beth A. , Kim Joonhoon , Pomraning Kyle R. 
Published: October 2024

CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering. In filamentous fungi, both Cas9 and Cas12a have been utilized as CRISPR nucleases. In this work we first compared efficacies and types of genetic edits for CRISPR-Cas9 and -Cas12a systems at the polyketide synthase (albA) gene locus in Aspergillus niger. By employing a tRNA-based gRNA polycistronic cassette, both Cas9 and Cas12a have demonstrated equally remarkable editing efficacy. Cas12a showed potential superiority over Cas9 protein when one gRNA was used for targeting, achieving an editing efficiency of 86.5% compared to 31.7% for Cas9. Moreover, when employing two gRNAs for targeting, both systems achieved up to 100% editing efficiency for single gene editing. In addition, the CRISPR-Cas9 system has been reported to induce large genomic deletions in various species. However, its use for engineering large chromosomal segments deletions in filamentous fungi still requires optimization. Here, we engineered Cas9 and -Cas12a-induced large genomic fragment deletions by targeting various genomic regions of A. niger ranging from 3.5 kb to 40 kb. Our findings demonstrate that targeted engineering of large chromosomal segments can be achieved, with deletions of up to 69.1% efficiency. Furthermore, by targeting a secondary metabolite gene cluster, we show that fragments over 100 kb can be efficiently and specifically deleted using the CRISPR-Cas9 or -Cas12a system. Overall, in this paper, we present an efficient multi-gRNA genome editing system utilizing Cas9 or Cas12a that enables highly efficient targeted editing of genes and large chromosomal regions in A. niger.
Current advances in the development of microRNA-integrated tissue engineering strategies: a cornerstone of regenerative medicine
Authors: Castañón-Cortés Luis Germán , Bravo-Vázquez Luis Alberto , Santoyo-Valencia Grecia , Medina-Feria Sara , Sahare Padmavati , Duttaroy Asim K. , Paul Sujay 
Published: October 2024

Regenerative medicine is an innovative scientific field focused on repairing, replacing, or regenerating damaged tissues and organs to restore their normal functions. A central aspect of this research arena relies on the use of tissue-engineered scaffolds, which serve as structural supports that mimic the extracellular matrix, providing an environment that orchestrates cell growth and tissue formation. Remarkably, the therapeutic efficacy of these scaffolds can be improved by harnessing the properties of other molecules or compounds that have crucial roles in healing and regeneration pathways, such as phytochemicals, enzymes, transcription factors, and non-coding RNAs (ncRNAs). In particular, microRNAs (miRNAs) are a class of tiny (20–24 nt), highly conserved ncRNAs that play a critical role in the regulation of gene expression at the post-transcriptional level. Accordingly, miRNAs are involved in a myriad of biological processes, including cell differentiation, proliferation, and apoptosis, as well as tissue regeneration, angiogenesis, and osteogenesis. On this basis, over the past years, a number of research studies have demonstrated that miRNAs can be integrated into tissue-engineered scaffolds to create advanced therapeutic platforms that precisely modulate cellular behavior and offer a controlled and targeted release of miRNAs to optimize tissue repair and regeneration. Therefore, in this current review, we discuss the most recent advances in the development of miRNA-loaded tissue-engineered scaffolds and provide an overview of the future outlooks that should be aborded in this area of study in order to lay the groundwork for the clinical translation of these tissue engineering approaches.
Interleukin-1 receptor-dependent and -independent caspase-1 activity in retinal cells mediated by receptor interacting protein 2
Authors: Coughlin Brandon A. , Christian Barbara , Trombley Brett , Mohr Susanne 
Published: October 2024

IntroductionInflammation and cell death play an important role in the pathogenesis of diabetic retinopathy. Previously we observed sustained activation of pro-inflammatory caspase-1 in retinas of diabetic animals and patients. In this study, we aimed to look at mechanisms underlying chronic caspase-1 activation in vitro and in vivo.MethodsNon-diabetic and diabetic wild type and IL-1 receptor (IL-1R1) knockout mice were used for in vivo experiments. Diabetes was induced using STZ (streptozotocin). Human Müller cells were used for in vitro studies. Cells were treated with either 5 mM or 25 mM glucose or interleukin-1beta (IL-1β) in the presence or absence of IL-1 receptor antagonist (IL-1ra) or siRNA against RIP2 (receptor interacting protein-2) for up to 96 h. Outcome measurements to assess Müller cell functions included measurements of caspase-1 activity using a fluorescence peptide substrate, production of IL-1β by Elisa, and cell death using trypan blue exclusion assays.ResultsOur in vivo results demonstrate that caspase-1 activation progresses from an IL-1R1 independent mechanism at 10 weeks of diabetes to an IL-1R1 dependent mechanism at 20 weeks indicating that feedback through IL-1R1 is crucial for sustained caspase-1 activity in retinas of mice. A similar hyperglycemia-mediated caspase-1/IL-1β/IL-1R1 feedback signaling was detected in vitro in human Müller cells which was prevented by treatment with IL-1ra. Our data also indicate that hyperglycemia induces caspase-1 activation initially but IL-1β sustains caspase-1 activation via caspase-1/IL-1β/IL-1R1 feedback and we identified RIP2 as mediator for both hyperglycemia- and IL-1β-induced caspase-1 activation. Activation of caspase-1/IL-1β/IL-1R1 feedback signaling caused Müller cell death which was prevented by RIP2 knockdown.DiscussionWe conclude that any intervention in caspase-1/IL-1β/IL-1R1 feedback signaling presents novel therapeutic options for the treatment of diabetic retinopathy.