bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors that Influence Session-Rating of Perceived Exertion in Elite Gaelic Football
Authors: Malone Shane , Hughes Brian , Roe Mark , Mangan Shane , Collins Kieran 
Published: August 2017

Abstract
Malone, S, Hughes, B, Roe, M, Mangan, S, and Collins, K. Factors that influence session-rating of perceived exertion in elite Gaelic football. J Strength Cond Res 34(4): 1176–1183, 2020—The current investigation examined the relationships between external training load (TL) measures and the session rating of perceived exertion (s-RPE) within Gaelic football players. Furthermore, we examined the effect that player experience, playing position, and 1-km time trial (TT) performance had on perception of TL. Physical performance (total distance, high-speed distance, very high–speed distance, max velocity, total accelerations, and maximal velocity exposures) and perceived TL (s-RPE) data were collected from 45 elite intercounty Gaelic football players (mean ± SD age of 24.2 ± 2.9 years; height: 180 ± 7 cm; mass: 81 ± 7 kg) over a 1-year period this resulted in 4,095 individual training session data being collected. There were moderate to very large associations between s-RPE and distance measures. Post hoc analysis revealed that the 0–1-year group had a higher s-RPE TL than the 2–3-year (d = 0.84 ± 0.33, small), 4–6-year (d = 0.91 ± 0.30, small), and 7+ year (d = 0.81 ± 0.30, small) groups. Midfielders reported higher s-RPE when compared with all positions (d = 1.21 ± 0.21, moderate). Half-backs (d = 0.81 ± 0.30, small), and half-forwards (d = 0.84 ± 0.31, small) had a higher s-RPE TL than the full-backs and full-forwards when external TL was accounted for (d = 1.21 ± 0.58, moderate). When aerobic fitness was considered, a 1.2% AU increase in s-RPE TL per 1 second increase in TT time (90% confidence limits: 1.07–2.64) when external TL was held constant was observed. The current investigation has shown moderate to very large associations between s-RPE and distance measures. When experience and fitness were considered as potential moderators of TL, it was observed that a given external TL may result in different internal responses between athletes. This potentially leaves individuals at risk of overtraining or failing to elicit positive adaptation. It is therefore vital that coaches and trainers give consideration to these mediators of s-RPE TL.
Halftime Rewarm-up With Intermittent Exercise Improves the Subsequent Exercise Performance of Soccer Referees
Authors: Yanaoka Takuma , Yamagami Jumpei , Kidokoro Tetsuhiro , Kashiwabara Kyoko , Miyashita Masashi 
Published: August 2017

Abstract
Yanaoka, T, Yamagami, J, Kidokoro, T, Kashiwabara, K, and Miyashita, M. Halftime rewarm-up with intermittent exercise improves the subsequent exercise performance of soccer referees. J Strength Cond Res 32(1): 211–216, 2018—This study investigated the effect of halftime rewarm-up (RW) with intermittent exercise on the subsequent exercise performance of soccer referees, determined by the Yo-Yo Intermittent Recovery Test level 1 (Yo-Yo IR1). Using a randomized cross-over design, 10 male referees were required to complete 2 trials. The trials consisted of the Loughborough Intermittent Shuttle Test, halftime, and Yo-Yo IR1 periods. During halftime, participants either rested on a chair (Control) or performed a halftime RW exercise for 15 minutes. The halftime RW protocol comprised 2.15 minutes of seated rest, followed by 2.15 minutes of running at 70% of the maximum heart rate (HRmax)—this cycle of recovery and running was repeated for a total of 13 minutes. The halftime RW protocol started at 1 minute after the commencement of the halftime period and concluded 1 minute before its end. The Yo-Yo IR1 performance, blood glucose, free fatty acids (FFAs), triglycerides (TGs), creatine kinase (CK), and lactate concentrations, the rating of perceived exertion, mean HR, and HRmax were analyzed. The Yo-Yo IR1 performance was higher in the halftime RW trial than in the control trial (3,095 ± 326 vs. 2,904 ± 421 m, P ≤ 0.05). The mean HR and HRmax, blood glucose, FFA, TG, CK, and lactate concentrations did not differ between the trials. The rating of perceived exertion during the halftime RW, but not after the Yo-Yo IR1 period, was higher than that in the control trial. In conclusion, this study showed that halftime RW with intermittent exercise improves the subsequent exercise performance.
Repulsive Guidance Molecule a (RGMa) Induces Neuropathological and Behavioral Changes That Closely Resemble Parkinson's Disease
Authors: Korecka Joanna A. , Moloney Elizabeth B. , Eggers Ruben , Hobo Barbara , Scheffer Sanny , Ras-Verloop Nienke , Pasterkamp R. Jeroen , Swaab Dick F. , Smit August B. , van Kesteren Ronald E. , Bossers Koen , Verhaagen Joost 
Published: August 2017

Repulsive guidance molecule member a (RGMa) is a membrane-associated or released guidance molecule that is involved in axon guidance, cell patterning, and cell survival. In our previous work, we showed that RGMa is significantly upregulated in the substantia nigra of patients with Parkinson's disease. Here we demonstrate the expression of RGMa in midbrain human dopaminergic (DA) neurons. To investigate whether RGMa might model aspects of the neuropathology of Parkinson's disease in mouse, we targeted RGMa to adult midbrain dopaminergic neurons using adeno-associated viral vectors. Overexpression of RGMa resulted in a progressive movement disorder, including motor coordination and imbalance, which is typical for a loss of DA release in the striatum. In line with this, RGMa induced selective degeneration of dopaminergic neurons in the substantia nigra (SN) and affected the integrity of the nigrostriatal system. The degeneration of dopaminergic neurons was accompanied by a strong microglia and astrocyte activation. The behavioral, molecular, and anatomical changes induced by RGMa in mice are remarkably similar to the clinical and neuropathological hallmarks of Parkinson's disease. Our data indicate that dysregulation of RGMa plays an important role in the pathology of Parkinson's disease, and antibody-mediated functional interference with RGMa may be a disease modifying treatment option.SIGNIFICANCE STATEMENTParkinson's disease (PD) is a neurodegenerative disease characterized by severe motor dysfunction due to progressive degeneration of mesencephalic dopaminergic (DA) neurons in the substantia nigra. To date, there is no regenerative treatment available. We previously showed that repulsive guidance molecule member a (RGMa) is upregulated in the substantia nigra of PD patients. Adeno-associated virus-mediated targeting of RGMa to mouse DA neurons showed that overexpression of this repulsive axon guidance and cell patterning cue models the behavioral and neuropathological characteristics of PD in a remarkable way. These findings have implications for therapy development as interfering with the function of this specific axon guidance cue may be beneficial to the survival of DA neurons.
Effect of Threat on Right dlPFC Activity during Behavioral Pattern Separation
Authors: Balderston Nicholas L. , Hsiung Abigail , Ernst Monique , Grillon Christian 
Published: August 2017

It has long been established that individuals with anxiety disorders tend to overgeneralize attributes of fearful stimuli to nonfearful stimuli, but there is little mechanistic understanding of the neural system that supports overgeneralization. To address this gap in our knowledge, this study examined effect of experimentally induced anxiety in humans on generalization using the behavioral pattern separation (BPS) paradigm. Healthy subjects of both sexes encoded and retrieved novel objects during periods of safety and threat of unpredictable shocks while we recorded brain activity with fMRI. During retrieval, subjects were instructed to differentiate among new, old, and altered images. We hypothesized that the hippocampus and dorsolateral prefrontal cortex (dlPFC) would play a key role in the effect of anxiety on BPS. The dlPFC, but not the hippocampus, showed increased activity for altered images compared with old images when retrieval occurred during periods of threat compared with safety. In addition, accuracy for altered items retrieved during threat was correlated with dlPFC activity. Together, these results suggest that overgeneralization in anxiety patients may be mediated by an inability to recruit the dlPFC, which mediates the cognitive control needed to overcome anxiety and differentiate between old and altered items during periods of threat.SIGNIFICANCE STATEMENTAnxiety and posttraumatic stress disorder patients generalize fear to nonfearful fear stimuli, making it difficult to regulate anxiety. Understanding how anxiety affects generalization is key to understanding the overgeneralization experienced by these patients. We examined this relationship in healthy subjects by studying how threat of shock affects neural responses to previously encountered stimuli. Although previous studies point to hippocampal involvement, we found that threat affected activity in the dorsolateral prefrontal cortex (dlPFC), rather than the hippocampus, when subjects encountered slightly altered versions of the previously encountered items. Importantly, this dlPFC activity predicted performance for these items. Together, these results suggest that the dlPFC is important for discrimination during elevated anxiety and that overgeneralization may reflect a deficit in dlPFC-mediated cognitive control.
Conditional Deletion ofPrnpRescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease
Authors: Salazar Santiago V. , Gallardo Christopher , Kaufman Adam C. , Herber Charlotte S. , Haas Laura T. , Robinson Sophie , Manson Jean C. , Lee Michael K. , Strittmatter Stephen M. 
Published: August 2017

Biochemical and genetic evidence implicate soluble oligomeric amyloid-β (Aβo) in triggering Alzheimer's disease (AD) pathophysiology. Moreover, constitutive deletion of the Aβo-binding cellular prion protein (PrPC) prevents development of memory deficits in APPswe/PS1ΔE9 mice, a model of familial AD. Here, we define the role of PrPCto rescue or halt established AD endophenotypes in a therapeutic disease-modifying time window after symptom onset. Deletion ofPrnpat either 12 or 16 months of age fully reverses hippocampal synapse loss and completely rescues preexisting behavioral deficits by 17 months. In contrast, but consistent with a neuronal function for Aβo/PrPCsignaling, plaque density, microgliosis, and astrocytosis are not altered. Degeneration of catecholaminergic neurons remains unchanged by PrPCreduction after disease onset. These results define the potential of targeting PrPCas a disease-modifying therapy for certain AD-related phenotypes after disease onset.SIGNIFICANCE STATEMENTThe study presented here further elucidates our understanding of the soluble oligomeric amyloid-β–Aβo-binding cellular prion protein (PrPC) signaling pathway in a familial form of Alzheimer's disease (AD) by implicating PrPCas a potential therapeutic target for AD. In particular, genetic deletion ofPrnprescued several familial AD (FAD)-associated phenotypes after disease onset in a mouse model of FAD. This study underscores the therapeutic potential of PrPCdeletion given that patients already present symptoms at the time of diagnosis.
Dedicated Hippocampal Inhibitory Networks for Locomotion and Immobility
Authors: Arriaga Moises , Han Edward B. 
Published: August 2017

Network activity is strongly tied to animal movement; however, hippocampal circuits selectively engaged during locomotion or immobility remain poorly characterized. Here we examined whether distinct locomotor states are encoded differentially in genetically defined classes of hippocampal interneurons. To characterize the relationship between interneuron activity and movement, we usedin vivo, two-photon calcium imaging in CA1 of male and female mice, as animals performed a virtual-reality (VR) track running task. We found that activity in most somatostatin-expressing and parvalbumin-expressing interneurons positively correlated with locomotion. Surprisingly, nearly one in five somatostatin or one in seven parvalbumin interneurons were inhibited during locomotion and activated during periods of immobility. Anatomically, the somata of somatostatin immobility-activated neurons were smaller than those of movement-activated neurons. Furthermore, immobility-activated interneurons were distributed across cell layers, with somatostatin-expressing cells predominantly in stratum oriens and parvalbumin-expressing cells mostly in stratum pyramidale. Importantly, each cell's correlation between activity and movement was stable both over time and across VR environments. Our findings suggest that hippocampal interneuronal microcircuits are preferentially active during either movement or immobility periods. These inhibitory networks may regulate information flow in “labeled lines” within the hippocampus to process information during distinct behavioral states.SIGNIFICANCE STATEMENTThe hippocampus is required for learning and memory. Movement controls network activity in the hippocampus but it's unclear how hippocampal neurons encode movement state. We investigated neural circuits active during locomotion and immobility and found interneurons were selectively active during movement or stopped periods, but not both. Each cell's response to locomotion was consistent across time and environments, suggesting there are separate dedicated circuits for processing information during locomotion and immobility. Understanding how the hippocampus switches between different network configurations may lead to therapeutic approaches to hippocampal-dependent dysfunctions, such as Alzheimer's disease or cognitive decline.
The Atypical MAP Kinase SWIP-13/ERK8 Regulates Dopamine Transporters through a Rho-Dependent Mechanism
Authors: Bermingham Daniel P. , Hardaway J. Andrew , Refai Osama , Marks Christian R. , Snider Sam L. , Sturgeon Sarah M. , Spencer William C. , Colbran Roger J. , Miller David M. , Blakely Randy D. 
Published: August 2017

The neurotransmitter dopamine (DA) regulates multiple behaviors across phylogeny, with disrupted DA signaling in humans associated with addiction, attention-deficit/ hyperactivity disorder, schizophrenia, and Parkinson's disease. The DA transporter (DAT) imposes spatial and temporal limits on DA action, and provides for presynaptic DA recycling to replenish neurotransmitter pools. Molecular mechanisms that regulate DAT expression, trafficking, and function, particularlyin vivo, remain poorly understood, though recent studies have implicated rho-linked pathways in psychostimulant action. To identify genes that dictate the ability of DAT to sustain normal levels of DA clearance, we pursued a forward genetic screen inCaenorhabditis elegansbased on the phenotype swimming-induced paralysis (Swip), a paralytic behavior observed in hermaphrodite worms with loss-of-functiondat-1mutations. Here, we report the identity ofswip-13, which encodes a highly conserved ortholog of the human atypical MAP kinase ERK8. We present evidence that SWIP-13 acts presynaptically to insure adequate levels of surface DAT expression and DA clearance. Moreover, we providein vitroandin vivoevidence supporting a conserved pathway involving SWIP-13/ERK8 activation of Rho GTPases that dictates DAT surface expression and function.SIGNIFICANCE STATEMENTSignaling by the neurotransmitter dopamine (DA) is tightly regulated by the DA transporter (DAT), insuring efficient DA clearance after release. Molecular networks that regulate DAT are poorly understood, particularlyin vivo. Using a forward genetic screen in the nematodeCaenorhabditis elegans, we implicate the atypical mitogen activated protein kinase, SWIP-13, in DAT regulation. Moreover, we providein vitroandin vivoevidence that SWIP-13, as well as its human counterpart ERK8, regulate DAT surface availability via the activation of Rho proteins. Our findings implicate a novel pathway that regulates DA synaptic availability and that may contribute to risk for disorders linked to perturbed DA signaling. Targeting this pathway may be of value in the development of therapeutics in such disorders.
Network analyses identify liver‐specific targets for treating liver diseases
Authors: Lee Sunjae , Zhang Cheng , Liu Zhengtao , Klevstig Martina , Mukhopadhyay Bani , Bergentall Mattias , Cinar Resat , Ståhlman Marcus , Sikanic Natasha , Park Joshua K , Deshmukh Sumit , Harzandi Azadeh M , Kuijpers Tim , Grøtli Morten , Elsässer Simon J , Piening Brian D , Snyder Michael , Smith Ulf , Nielsen Jens , Bäckhed Fredrik , Kunos George , Uhlen Mathias , Boren Jan , Mardinoglu Adil 
Published: August 2017

AbstractWe performed integrative network analyses to identify targets that can be used for effectively treating liver diseases with minimal side effects. We first generated co‐expression networks (CNs) for 46 human tissues and liver cancer to explore the functional relationships between genes and examined the overlap between functional and physical interactions. Since increased de novo lipogenesis is a characteristic of nonalcoholic fatty liver disease (NAFLD) and hepatocellular carcinoma (HCC), we investigated the liver‐specific genes co‐expressed with fatty acid synthase (FASN). CN analyses predicted that inhibition of these liver‐specific genes decreases FASN expression. Experiments in human cancer cell lines, mouse liver samples, and primary human hepatocytes validated our predictions by demonstrating functional relationships between these liver genes, and showing that their inhibition decreases cell growth and liver fat content. In conclusion, we identified liver‐specific genes linked to NAFLD pathogenesis, such as pyruvate kinase liver and red blood cell (PKLR), or to HCC pathogenesis, such as PKLR, patatin‐like phospholipase domain containing 3 (PNPLA3), and proprotein convertase subtilisin/kexin type 9 (PCSK9), all of which are potential targets for drug development.
IV-я СЕССИЯ МЕЖДУНАРОДНОЙ ШКОЛЫ КОРМОВ
Authors: Макаринская А. В. 
Published: August 2017

29 мая - 04 июня 2017 г. в рамках IV-ой сессии Международной школы кормов, организованной Одесской национальной академией пищевых технологий (ОНАПТ), проведено два тренинга: - Тренинг 1: «Искусство создания высокоэффективных рецептов комбикормов»; - Тренинг 2: «Внутренний аудит и оценка схемы GMP+FSA в соответствии с требованиями стандартов GMP+B1 “PRODUCTION, TRADE & SERVICES” и ISO 19011:2011».
Criteria for diagnosis of bacterial vaginosis using the test Femoflor-16
Authors: Nazarova Veronika V. , Shipitsyna Elena V. , Gerasimova Ekaterina N. , Savicheva Alevtina M. 
Published: August 2017

Background. Bacterial vaginosis is disturbance of the balance of the vaginal microflora, associated with a number of infectious diseases of the urogenital tract and adverse pregnancy outcomes. In this country, for the detection of vaginal dysbiotic conditions, the test Femoflor-16 (DNA-Technology, Moscow) is widely used, however interpretation algorithms of this test do not include the category of BV.
Aim. The study aimed to elaborate diagnostic criteria for the detection of BV using Femoflor-16 test.
Materials and methods. Women of reproductive age addressing a gynecologist with vaginal discharge were enrolled in the study. For clinical diagnosis of BV, the Amsel criteria were used, laboratory analysis for BV was performed via microscopic investigation of vaginal discharge using the Nugent score. Samples of vaginal discharge from all women were analyzed with the test Femoflor-16, intended for characterizing vaginal microbiocenosis using multiplex quantitative real-time PCR.
Results. A total of 280 women were included in the study. BV was diagnosed in 86 women (31%) using the Amsel criteria, and in 81 women (29%) using the Nugent score. All groups of anaerobic bacteria included in Femoflor-16 test were shown to be associated with BV, with the exception of bacteria of the genus Mobiluncus, which are detected together with phylogenetically related but not BV-associated bacteria of the genus Corynebacterium. A low amount of lactobacilli (< 10% of total bacterial load) coupled with an elevated amount of Gardnerella vaginalis/Prevotella bivia/Porphyromonas (> 1%) and/or Eubacterium (> 2%) and/or Sneathia/Leptotrichia/Fusobacterium (> 0.1%) and/or Megasphaera/Veillonella/Dialister (> 0.1%) and/or Lachnobacterium/Clostridium (> 0.1%) and/or Peptostreptococcus (> 0.1%) and/or Atopobium vaginae (> 0.2%) detected BV with a sensitivity of 99% and specificity of 93%.
Conclusions. Criteria for BV diagnosis using the test Femoflor-16 have been elaborated, which enable to detect BV or exclude it with a sensitivity of 99% and specificity of 93%. These criteria for BV and criteria of the test manufacturers for severe anaerobic dysbiosis determine to a large extent the same category of the vaginal microbiocenosis.
Ilio inguinal neurectomy in inguinal hernia
Authors: Mohanapriya T. , Karthikeyan T. R. , Singh K. Balaji , Arulappan T. 
Published: August 2017

Background: Inguinal hernia is one of the most common surgery done in any hospital. Chronic inguinal pain is one of the common problem these patients complain of. This study aims at evaluating the long-term outcomes of neuralgia and paraesthesia following routine ilioinguinal nerve division, compared to nerve preservation when performing Lichtenstein’s inguinal hernia repair.Methods: The present study is a randomized controlled trial of 60 cases of inguinal hernias admitted in General Surgery Department in our hospital. The patients were randomly chosen into Lichtenstein hernia repair with ilioinguinal neurectomy or Lichtenstein hernia repair with nerve preservation. After surgery, all patients were monitored carefully for pain and paraesthesia. Pain was assessed using visual analogue scale. Paraesthesia was assessed by the monofilament test and evaluated after comparison with the opposite side.Results: In the present study, the incidence of pain as well as the severity of pain is far higher in the nerve preservation study group as opposed to the neurectomy study group. Hypaesthesia is not a significant complication following ilioinguinal neurectomy and does not significantly add to the morbidity of the patient.Conclusions: Prophylactic neurectomy can be an appropriate solution in the prevention of chronic groin pain following Lichtenstein inguinal hernia repair and can be considered as an ideal inclusion into the standard hernia repair procedures. Hypoesthesia is not a significant complication following ilioinguinal neurectomy and does not significantly add to the morbidity of the patient.