bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scent marking in Sunda clouded leopards (Neofelis diardi): novel observations close a key gap in understanding felid communication behaviours
Authors: Allen Maximilian L. , Wittmer Heiko U. , Setiawan Endro , Jaffe Sarah , Marshall Andrew J. 
Published: October 2016

AbstractIntraspecific communication is integral to the behavioural ecology of solitary carnivores, but observing and quantifying their communication behaviours in natural environments is difficult. Our systematic literature review found that basic information on scent marking is completely lacking for 23% of all felid species, and information on 21% of other felid species comes solely from one study of captive animals. Here we present results of the first systematic investigation of the scent marking behaviours of Sunda clouded leopards in the wild. Our observations using motion-triggered video cameras in Indonesian Borneo are novel for clouded leopards, and contrary to previous descriptions of their behaviour. We found that clouded leopards displayed 10 distinct communication behaviours, with olfaction, scraping, and cheek rubbing the most frequently recorded. We also showed that males make repeated visits to areas they previously used for marking and that multiple males advertise and receive information at the same sites, potentially enhancing our ability to document and monitor clouded leopard populations. The behaviours we recorded are remarkably similar to those described in other solitary felids, despite tremendous variation in the environments they inhabit, and close a key gap in understanding and interpreting communication behaviours of clouded leopards and other solitary felids.
Electronically-Controlled Beam-Steering through Vanadium Dioxide Metasurfaces
Authors: Hashemi Mohammed Reza M. , Yang Shang-Hua , Wang Tongyu , Sepúlveda Nelson , Jarrahi Mona 
Published: October 2016

AbstractEngineered metamaterials offer unique functionalities for manipulating the spectral and spatial properties of electromagnetic waves in unconventional ways. Here, we report a novel approach for making reconfigurable metasurfaces capable of deflecting electromagnetic waves in an electronically controllable fashion. This is accomplished by tilting the phase front of waves through a two-dimensional array of resonant metasurface unit-cells with electronically-controlled phase-change materials embedded inside. Such metasurfaces can be placed at the output facet of any electromagnetic radiation source to deflect electromagnetic waves at a desired frequency, ranging from millimeter-wave to far-infrared frequencies. Our design does not use any mechanical elements, external light sources, or reflectarrays, creating, for the first time, a highly robust and fully-integrated beam-steering device solution. We demonstrate a proof-of-concept beam-steering metasurface optimized for operation at 100 GHz, offering up to 44° beam deflection in both horizontal and vertical directions. Dynamic control of electromagnetic wave propagation direction through this unique platform could be transformative for various imaging, sensing, and communication applications, among others.
Characteristics of a water-forming NADH oxidase from Methanobrevibacter smithii, an archaeon in the human gut
Authors: Yan Mingguang , Yin Weibing , Fang Xiao , Guo Jianjun , Shi Hong 
Published: October 2016

NADH oxidases (NOXs) catalysing the oxidation of NADH to yield NAD+ and H2O, H2O2, or both play an important role in protecting organisms from oxidative stress and maintaining the balance of NAD+/NADH. A gene encoding NOX was identified from Methanobrevibacter smithii (NOX-ms), the predominant archaeon in the human gut ecosystem. Subsequent analyses showed that it is an FAD-containing protein with a subunit molecular mass of 48 kDa. NOX-ms was purified to homogeneity after expression in Escherichia coli. NOX-ms catalysed the oxidization of NADH and converted O2 to H2O with an optimal pH of 7.5 and a temperature optimum of approximately 37°C. The Vmax and Km values were 42.6–44.1 unit/mg and 47.8–54.6 μM for NADH. The apparent Vmax and Km for oxygen were 189.5–196.1 unit/mg and 14.6–16.8 μM. The mutation analysis suggests that Cys42 in NOX-ms plays a key role in the four-electron reduction of O2 to H2O. Quantitative reverse transcription-PCR (RT-qPCR) revealed that transcription of NOX-ms was also up-regulated after exposing the cells to oxidative stress and glucose. Finally, the potential of NOX-ms as a target to control colonization of M. smithii and its possible applications are discussed.
A Lota lota Consumption: Trophic Dynamics of Nonnative Burbot in a Valuable Sport Fishery
Authors: Klobucar Stephen L. , Saunders W. Carl , Budy Phaedra 
Published: October 2016

AbstractUnintentional and illegal introductions of species disrupt food webs and threaten the success of managed sport fisheries. Although many populations of Burbot Lota lota are declining in the species’ native range, a nonnative population recently expanded into Flaming Gorge Reservoir (FGR), Wyoming–Utah, and threatens to disrupt predator–prey interactions within this popular sport fishery. To determine potential impacts on sport fishes, especially trophy Lake Trout Salvelinus namaycush, we assessed the relative abundance of Burbot and quantified the potential trophic or food web impacts of this population by using diet, stable isotope, and bioenergetic analyses. We did not detect a significant potential for food resource competition between Burbot and Lake Trout (Schoener's overlap index = 0.13), but overall consumption by Burbot likely affects other sport fishes, as indicated by our analyses of trophic niche space. Diet analyses suggested that crayfish were important diet items across time (89.3% of prey by weight in autumn; 49.4% in winter) and across Burbot size‐classes (small: 77.5% of prey by weight; medium: 76.6%; large: 39.7%). However, overall consumption by Burbot increases as water temperatures cool, and fish consumption by Burbot in FGR was observed to increase during winter. Specifically, large Burbot consumed more salmonids, and we estimated (bioenergetically) that up to 70% of growth occurred in late autumn and winter. Further, our population‐wide consumption estimates indicated that Burbot could consume up to double the biomass of Rainbow Trout Oncorhynchus mykiss stocked annually (>1.3 × 105 kg; >1 million individuals) into FGR. Overall, we provide some of the first information regarding Burbot trophic interactions outside of the species’ native range; these findings can help to inform the management of sport fisheries if Burbot range expansion occurs elsewhere.Received March 18, 2016; accepted August 15, 2016 Published online October 14, 2016
Allgöwer–Donati Versus Vertical Mattress Suture Technique Impact on Perfusion in Ankle Fracture Surgery: A Randomized Clinical Trial Using Intraoperative Angiography
Authors: Shannon Steven F. , Houdek Matthew T. , Wyles Cody C. , Yuan Brandon J. , Cross William W. , Cass Joseph R. , Sems Stephen A. 
Published: October 2016

Objective:
The purpose of this study was to evaluate which primary wound closure technique for ankle fractures affords the most robust perfusion as measured by laser-assisted indocyanine green angiography: Allgöwer–Donati or vertical mattress.

Design:
Prospective, randomized.

Setting:
Level 1 Academic Trauma Center.

Patients/Participants:
Thirty patients undergoing open reduction internal fixation for ankle fractures were prospectively randomized to Allgöwer–Donati (n = 15) or vertical mattress (n = 15) closure. Demographics were similar for both cohorts with respect to age, sex, body mass index, surgical timing, and OTA/AO fracture classification.

Main Outcome Measurements:
Skin perfusion (mean incision perfusion and mean perfusion impairment) was quantified in fluorescence units with laser-assisted indocyanine green angiography along the lateral incision as well as anterior and posterior to the incision at 30 separate locations. Minimum follow-up was 3 months with a mean follow-up 4.7 months.

Results:
Allgöwer–Donati enabled superior perfusion compared with the vertical mattress suture technique. Mean incision perfusion for Allgöwer–Donati was 51 (SD = 13) and for vertical mattress was 28 (SD = 10, P < 0.0001). Mean perfusion impairment was less in the Allgöwer–Donati cohort (12.8, SD = 9) compared with that in the vertical mattress cohort (23.4, SD = 14; P = 0.03). One patient in each cohort experienced a wound complication.

Conclusions:
The Allgöwer–Donati suture technique offers improved incision perfusion compared with vertical mattress closure after open reduction internal fixation of ankle fractures. Theoretically, this may enhance soft tissue healing and decrease the risk of wound complications. Surgeons may take this into consideration when deciding closure techniques for ankle fractures.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
APC Injuries With Symphyseal Fixation: What Affects Outcome?
Authors: Lybrand Kyle , Bell Anthony , Rodericks Deirdre , Templeman David , Tornetta Paul 
Published: October 2016

Objective:
To evaluate the influence of the symphyseal position at union, implant failure, and the type of posterior ring injury on validated outcome measures.

Design:
Retrospective review with prospectively collected validated outcome data.

Setting:
Two academic level 1 trauma centers.

Patients/Participants:
We evaluated 54 patients with operatively treated anterior-posterior compression (APC) type 2 and 3 injuries.

Intervention:
Thirty-five APC type 2 and 19 APC type 3 injuries were reviewed at a minimum of 2 years after surgery. Average follow-up was 7 years.

Main Outcome Measures:
Patients were evaluated with validated EuroQol five dimensions (EQ5D), EuroQol health index, Visual Analog Score (VAS) pain, Majeed pelvic scores, and change in work status. The final anterior-posterior (AP) radiograph available was reviewed for implant failure and displacement. Revision surgery was documented based on implant status and displacement at final follow-up.

Results:
There were trends toward better outcomes for APC type 2 for EQ5D and VAS pain. Patients with injury severity score (ISS) >16 had worse reported health, Majeed scores, and VAS pain. Nineteen patients had failure of fixation. There were no differences in any outcome measure; trends toward better Majeed score were found for patients with intact fixation. Displacements >15 mm anteriorly at final follow-up negatively affect outcomes with significantly worse EQ5D, reported health, and Majeed score. Two patients required revision surgery. There were no differences in final outcomes.

Conclusions:
No significant differences were found for APC type 2 versus type 3 injuries. Higher injury severity score resulted in worse outcomes and more pain. Outcomes were not effected by implant failure; however, major loss of reduction (>15 mm) anteriorly did negatively impact outcomes. Patients with failure who were revised to union did not have worse outcomes.

Level of Evidence:
Prognostic level IV. See Instructions for Authors for a complete description of levels of evidence.
Microneedling: A Comprehensive Review
Authors: Hou Angela , Cohen Brandon , Haimovic Adele , Elbuluk Nada 
Published: October 2016

BACKGROUND
Microneedling is a minimally invasive procedure that uses fine needles to puncture the epidermis. The microwounds created stimulate the release of growth factors and induce collagen production. The epidermis remains relatively intact, therefore helping to limit adverse events. The indications for microneedling therapy have grown significantly, and it is becoming a more widely used treatment in dermatology.

OBJECTIVE
A comprehensive review of microneedling in human subjects and its applications in dermatology.

METHODS AND MATERIALS
A search was performed using PubMed/MEDLINE and Science Direct databases. Search terms included “microneedling,” “needling,” and “percutaneous collagen induction.” All available studies involving human subjects were included in the discussion, with priority given to prospective, randomized trials.

RESULTS
Studies demonstrate microneedling efficacy and safety for the treatment of scars, acne, melasma, photodamage, skin rejuvenation, hyperhidrosis and alopecia and for facilitation of transdermal drug delivery. While permanent adverse events are uncommon, transient erythema and postinflammatory hyperpigmentation are more commonly reported.

CONCLUSION
Microneedling appears to be an overall effective and safe therapeutic option for numerous dermatologic conditions. Larger and more randomized controlled trials are needed to provide greater data on the use of microneedling for different dermatologic conditions in different skin types.
The Relationship Between Morphology and Function of the Meibomian Glands
Authors: Kim Hyo Myung , Eom Youngsub , Song Jong Suk 
Published: October 2016

Abstract:
Meibomian gland dysfunction (MGD) is a leading cause of dry eye disease (DED). Meibomian gland dysfunction is divided into two major categories based on meibomian gland secretion: low delivery and high delivery. The low-delivery states are further classified as either hyposecretory or obstructive subtype, and the high-delivery state is termed “hypersecretory MGD.” Two parameters are commonly used to evaluate meibomian gland function: lipid layer thickness and meibum quality and expressibility. To evaluate the morphology of meibomian glands, meibography is used to detect meibomian gland dropout. Functional and morphological changes of meibomian glands are often thought to be well correlated; in reality, many cases do not because some patients have characteristics of both hypersecretory and obstructive subtypes. We suggest that the MGD classification system should be modified to include a mixed subtype to explain such patients.
Biofilm Formation on Bandage Contact Lenses Worn by Patients with the Boston Type 1 Keratoprosthesis: A Pilot Comparison Study of Prophylactic Topical Vancomycin 15 mg/mL and Linezolid 0.2%
Authors: Farooq Asim V. , Hou Joshua H. , Jassim Sarmad , Haq Zeeshan , Tu Elmer Y. , de la Cruz Jose , Cortina Maria S. 
Published: October 2016

Objectives:
To determine the rate of biofilm formation on bandage contact lenses worn by patients with the Boston type 1 keratoprosthesis (K-Pro) while on prophylactic topical vancomycin versus linezolid.

Methods:
Patients wearing a bandage contact lens (BCL) with a K-Pro were eligible for enrollment. After irrigation of the ocular surface with 5% povidone-iodine solution, each patient was placed on either topical vancomycin 15 mg/mL or linezolid 0.2% BID for one month. At the one-month visit, the BCL was collected and stored in fixative solution. Standard photographs were taken of each lens at high magnification using scanning electron microscopy (SEM), which were subsequently analyzed for evidence of biofilm.

Results:
Nineteen contact lenses were obtained from 12 K-Pro patients at the Illinois Eye and Ear Infirmary. Zero of eight (0%; 95% CI=0 to 37%) contact lenses from patients treated with topical vancomycin, and 1 of 11 (9%; 95% CI=0 to 41%; P-value=1.00) contact lenses from patients treated with topical linezolid were found to have biofilm formation at one month as detected by SEM. None of the patients developed a clinically significant infection while on either prophylactic vancomycin or linezolid during the study period.

Conclusions:
Overall, the rate of biofilm formation as detected by SEM on the surface of bandage contact lenses was low. These results suggest that vancomycin and linezolid are both relatively effective in reducing biofilm-forming bacterial growth at one month. Accordingly, linezolid may be an effective alternative to vancomycin in patients with allergy or intolerance. However, further investigation is required to develop evidence-based antibiotic prophylaxis regimens.
Mooren Ulcer in a Child Wearing Orthokeratology Contact Lenses
Authors: Kim Jaeyoung , Kim Mee Kum , Wee Won Ryang , Oh Joo Youn 
Published: October 2016

Purpose:
To report a case of Mooren ulcer that developed in a pediatric patient wearing orthokeratology overnight contact lenses.

Methods:
Case report.

Results:
A 10-year-old boy was referred to our clinic because of progressive peripheral corneal ulcer in the right eye, despite the intensive use of fortified antibiotic eye drops. The patient had been using overnight orthokeratology lenses for 4 months before presentation of corneal ulcer. There was no other history of ocular or systemic trauma and disorders. Microbiological tests of the lesion were negative. Systemic evaluation showed no sign of rheumatologic disease. Under a diagnosis of Mooren ulcer, the patient was treated with topical and systemic corticosteroids. After four weeks of treatment, the patient's symptoms rapidly disappeared, and corneal ulcer was healed. The vision recovered to normal with the correction of with-the-rule astigmatism.

Conclusions:
Mooren ulcer can develop in pediatric patients wearing orthokeratology contact lenses. Given rapid progression of Mooren ulcer in a young population, early diagnosis and proper treatment are essential to prevent a devastating outcome.
Bayesian Analysis of High Throughput Data
Authors: Ma Eric J. , Hussein Islam T. M. , Zhong Vivian J. , Bandoro Christopher , Runstadler Jonathan A. 
Published: October 2016

AbstractDuplicate or triplicate experimental replicates are commonplace in the high throughput literature. However, it has not been tested whether this is statistically defensible or not. To address this issue, we use probabilistic programming to develop a simple hierarchical model for analyzing high throughput measurement data. With the model and simulated data, we show that a small increase in replicate experiments can quantitatively improve accuracy in measurement. We also provide posterior densities for statistical parameters used in the evaluation of HT data. Finally, we provide an extensible open source implementation that ingests data structured in a simple format and produces posterior densities of estimated measurement and assay evaluation parameters.
InterPred: A pipeline to identify and model protein-protein interactions
Authors: Mirabello Claudio , Wallner Björn 
Published: October 2016

AbstractProtein-protein interactions (PPI) are crucial for protein function. There exist many techniques to identify PPIs experimentally, but to determine the interactions in molecular detail is still difficult and very time-consuming. The fact that the number of PPIs is vastly larger than the number of individual proteins makes it practically impossible to characterize all interactions experimentally. Computational approaches that can bridge this gap and predict PPIs and model the interactions in molecular detail are greatly needed. Here we present InterPred, a fully automated pipeline that predicts and model PPIs from sequence using structural modelling combined with massive structural comparisons and molecular docking. A key component of the method is the use of a novel random forest classifier that integrate several structural features to distinguish correct from incorrect protein-protein interaction models. We show that InterPred represents a major improvement in protein-protein interaction detection with a performance comparable or better than experimental high-throughput techniques. We also show that our full-atom protein-protein complex modelling pipeline performs better than state of the art protein docking methods on a standard benchmark set. In addition, InterPred was also one of the top predictors in the latest CAPRI37 experiment.InterPred source code can be downloaded fromhttp://wallnerlab.org/InterPred
Topological features of gene regulatory networks predict patterns of natural diversity in environmental response
Authors: Des Marais David L. , Guerrero Rafael F. , Lasky Jesse R. , Scarpino Samuel V. 
Published: October 2016

AbstractMolecular interactions affect the evolution of complex traits. For instance, adaptation may be constrained by pleiotropic or epistatic effects, both of which will be reflected in the structure of molecular interaction networks. To date, empirical studies investigating the role of molecular interactions in phenotypic evolution have been idiosyncratic, offering no clear patterns. Here, we investigated the network topology of genes putatively involved in local adaptation to two abiotic stressors—drought and cold—inArabidopsis thaliana. Our findings suggest that the gene-interaction topologies for both cold and drought stress response are non-random, with genes that show genetic variation in drought response (GxE) being significantly more peripheral and cold response genes being significantly more central than genes not involved in either response. We suggest that the observed topologies reflect different constraints on the genetic pathways involved in the assayed phenotypes. The approach presented here may inform predictive models linking genetic variation in molecular signaling networks with phenotypic variation, specifically traits involved in environmental response.Significance StatementOur study focuses on genes whose transcriptional activity exhibits genetic variation in response to the environment, or “GxE.” GxE is a widely observed phenomenon of critical importance to understanding the genotype-to-phenotype map, the evolution of natural populations, medical genetics, population response to climate change, and agricultural improvement. We investigated expression GxE in plant responses to two abiotic cues: cold and drought. We found that genes showing genetically variable response to cold stress are centrally located in regulatory networks whereas genes showing genetically variable response to drought stress are peripherally located in regulatory networks. This result suggests that selection is presented with vastly different mutational landscapes for shaping evolutionary or breeding response to these two important climatic factors
Plant functional groups within a tropical forest exhibit different wood functional anatomy
Authors: Apgaua Deborah M. G. , Tng David Y. P. , Cernusak Lucas A. , Cheesman Alexander W. , Santos Rubens M. , Edwards Will J. , Laurance Susan G. W. 
Published: October 2016

Summary

Understanding the anatomical basis of plant water transport in forest ecosystems is crucial for contextualizing community‐level adaptations to drought, especially in life‐form‐rich tropical forests. To provide this context, we explored wood functional anatomy traits related to plant hydraulic architecture across different plant functional groups in a lowland tropical rain forest.

We measured wood traits in 90 species from six functional groups (mature‐phase, understorey and pioneer trees; understorey and pioneer shrubs; vines) and related these traits to intrinsic water‐use efficiency (WUEi) as a measure of physiological performance. We also examined vessel size distribution patterns across groups to determine trade‐offs in theoretical hydraulic safety vs. efficiency.

Some plant functional groups exhibited significant differences in vessel parameters and WUEi. Vessel diameters in vines and pioneer trees were two‐ to threefold greater on average than in understorey trees and shrubs. Contrastingly, vessels in understorey trees and shrubs fell within the smaller size classes, suggesting greater safety mechanisms. In addition to these trends, large vessel dimensions were important predictors of WUEi among the functional groups.

We conclude that wood functional anatomy profiles varied across plant functional groups in a tropical rain forest. These groups can therefore serve as a framework for further investigations on structure–function relationships and a sound basis for modelling species responses to drought.

A lay summary is available for this article.