bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of type 2 diabetes in patients with insomnia: A population‐based historical cohort study
Authors: Lin Chia‐Ling , Chien Wu‐Chien , Chung Chi‐Hsiang , Wu Fei‐Ling 
Published: August 2017

AbstractBackgroundWe investigated the risk of type 2 diabetes mellitus (T2DM) in patients with and without insomnia.MethodsIn this historical cohort study, we performed a secondary analysis of data from 2001 to 2010, which was obtained from Taiwan's National Health Insurance Database. We developed a Cox proportional hazard regression model to estimate the effects of insomnia on T2DM risk. Kaplan‐Meier survival analysis was applied to compare the differences in the cumulative incidence of T2DM between the groups with and without insomnia.ResultsDuring the follow‐up period, the T2DM incidence rate of patients with insomnia was significantly higher than that of patients without insomnia (34.7 vs 24.3 per 1000 person‐years). Overall, patients with insomnia had a higher risk of T2DM than did patients without insomnia (adjusted hazard ratio, 1.16; 95% confidence interval [CI], 1.10‐1.19). Among patients aged younger than 40 years, those with insomnia had a higher risk of T2DM than did the comparison cohort (adjusted hazard ratio, 1.31; 95% CI, 1.14‐1.55). Compared with patients without insomnia, the risk tended to increase with the duration of follow‐up in patients with insomnia; when the insomnia duration was <4 years, 4 to 8 years, and >8 years, the risk of T2DM increased by 1.14, 1.38, and 1.51 times (95% CI, 1.03‐1.17, 1.15‐1.49, and 1.20‐1.86), respectively. Patients with insomnia had a higher risk of T2DM, and this risk was particularly pronounced among the younger (≤40 years) population.ConclusionChronic insomnia could be an important risk factor for T2DM.
Fins into limbs: Recent insights from sarcopterygian fish
Authors: Amaral Danielson B. , Schneider Igor 
Published: August 2017

SummaryLimbs with digits evolved as sarcopterygian fish transitioned to a terrestrial life, giving rise to modern tetrapods. Since the Devonian, most of the sarcopterygian fish diversity became extinct, with the only surviving representatives being two coelacanth and six lungfish species. As the sister group of tetrapods, sarcopterygian fish constitute the ideal models to address questions regarding the transition of vertebrates from water to land. However, distantly related yet experimentally amenable teleost fish species have instead become the organisms of choice for geneticists and developmental biologists. Comparative studies using teleosts, such as zebrafish, have greatly enriched our knowledge on the genetic and regulatory mechanisms underlying fin development. However, their highly derived fin anatomy provides limited insights on the origin of limbs and the teleost‐specific whole‐genome duplication represents a further complication to comparisons of gene function and regulation. In recent years, refined morphological and behavioral studies and access to lungfish embryos and availability of genetic resources have underscored the value of nontetrapod sarcopterygians as model organisms. Here we review recent studies using lungfishes and coelacanths that shed light on various aspects of the evolution of the tetrapod limb, including the origin of the tetrapod pelvis, limb musculature, digits, locomotion, and regenerative capacity.
Alterations in anterior–posterior patterning and its accompanying changes along the proximal‐distal axis during the fin‐to‐limb transition
Authors: Tanaka Mikiko 
Published: August 2017

SummaryThe evolution from fins to limbs was one of the most successful innovations for vertebrates, allowing them to vastly expand their behaviors and habitats. Fossil records suggest that morphological changes occurred not only along the proximal‐distal axis included appearance of the autopod, but also occurred along the anterior‐posterior axis included reductions in the size and number of basal bones and digits. This review focuses on recent progress in developmental and genetic studies aimed at elucidating the mechanisms underlying alteration of anterior‐posterior patterning and its accompanying changes along the proximal‐distal axis during the fin‐to‐limb transition.
Using genetic tools to estimate the prevalence of non‐native red deer (Cervus elaphus) in a Western European population
Authors: Frantz Alain C. , Zachos Frank E. , Bertouille Sabine , Eloy Marie‐Christine , Colyn Marc , Flamand Marie‐Christine 
Published: August 2017

AbstractGame species like the red deer have been subjected to anthropogenic impacts for centuries. Translocations are often carried out—sometimes illegally—not only for sporting purposes, but also to increase trophy quality, reduce inbreeding, or mitigate bottlenecks after excessive persecution. Apart from the blurring of large‐scale genetic structure, translocations without adequate quarantine measure risk introducing pathogens into potentially immunologically naïve populations. It is therefore important to understand the frequency of clandestine translocations. Identification of non‐autochthonous animals and their potential origin is often difficult and, in red deer, has been hampered by the lack of large‐scale genotypic datasets for comparison. In the present study, we make use of a recently published European‐wide microsatellite dataset to detect and quantify the presence of non‐autochthonous red deer in a large population sample (n = 1,780) from Central Europe (Belgium). Using factorial correspondence analysis, assignment tests and Bayesian clustering algorithms we arrive at an estimate of 3.7% non‐autochthonous animals (or their descendants). Some of these animals were assigned to a nearby French population and may have immigrated into Belgium naturally, but the large majority must have been introduced by humans. Our analysis pointed to the British Isles and Germany/Poland as the potential origin of many introduced deer, regions known to have been source populations for translocations in Europe and beyond. We found evidence for recreational hunters using carcasses from farmed deer to fulfill mandatory hunting quotas. Our study is the first to quantify the extent of human‐mediated introductions in a European game species at such a large scale with large and representative sample sizes.
Inotropic myocardial reserve deficiency is the predominant feature of exercise haemodynamics in cardiac amyloidosis
Authors: Clemmensen Tor Skibsted , Mølgaard Henning , Sörensen Jens , Eiskjaer Hans , Andersen Niels Frost , Mellemkjaer Søren , Andersen Mads Jønsson , Tolbod Lars Poulsen , Harms Hendrik J. , Poulsen Steen Hvitfeldt 
Published: August 2017

AbstractAimsThis study aimed to characterize invasive haemodynamics during exercise in subjects with cardiac amyloidosis (CA).Methods and resultsThe study population numbered 44 subjects. Group A (CA‐positive, n = 24) comprised wild‐type transthyretin patients (n = 10), familial transthyretin amyloidosis mutation carriers (ATTRm) with cardiac involvement (n = 5), and light‐chain amyloidosis patients with cardiac involvement (n = 9). Group B (CA‐negative, n = 20) comprised four healthy ATTRm subjects without cardiac involvement documented by 11C‐PIB positron emission tomography and 16 healthy controls. All subjects underwent a symptom‐limited, semi‐supine exercise test with expired gas analysis and simultaneous right heart catheterization. CA patients had lower peak oxygen consumption [15 ± 6 mL/min/kg bodyweight (bwt) vs. 33 ± 7 mL/min/kg bwt; P < 0.0001] than controls. Myocardial reserve during exercise was significantly reduced in CA patients as reflected in a small increase in stroke volume index (SVI) and cardiac index (CI) compared with controls [ΔSVI: 4 mL/m2 (range: −1 to 8) vs. 14 mL/m2 (range: 5–25); P < 0.0001; ΔCI: 2 ± 2 L/min vs. 7 ± 2 L/min; P < 0.0001]. During exercise, CA patients had significantly higher left and right ventricular filling pressures than controls. Furthermore, CA patients had severely impaired pulmonary arterial compliance (PAC) compared with controls [2.9 mL/mmHg (range: 2.1–4.5) vs. 7.5 mL/mmHg (range: 5.7–10.4); P < 0.0001].ConclusionsCardiac amyloid deposits are associated with severely reduced inotropic myocardial reserve and increased left and right ventricular filling pressures during exercise. Furthermore, CA subjects have severely reduced PAC, which may contribute to right heart failure and reduced exercise capacity.
The acute toxicity of major ion salts to Ceriodaphnia dubia. III. Mathematical models for mixture toxicity
Authors: Erickson Russell J. , Mount David R. , Highland Terry L. , Hockett J. Russell , Hoff Dale J. , Jenson Correne T. , Norberg-King Teresa J. , Peterson Kira N. 
Published: August 2017

Abstract

Based on previous research on the acute toxicity of major ions (Na+, K+, Ca2+, Mg2+, Cl–, SO42–, and HCO3–/CO32–) to Ceriodaphnia dubia, a mathematical model was developed for predicting the median lethal concentration (LC50) for any ion mixture, excepting those dominated by K-specific toxicity. One component of the model describes a mechanism of general ion toxicity to which all ions contribute and predicts LC50s as a function of osmolarity and Ca activity. The other component describes Mg/Ca-specific toxicity to apply when such toxicity exceeds the general ion toxicity and predicts LC50s as a function of Mg and Ca activities. This model not only tracks well the observed LC50s from past research used for model development but also successfully predicts LC50s from new toxicity tests on synthetic mixtures of ions emulating chemistries of various ion-enriched effluents and receiving waters. It also performs better than a previously published model for major ion toxicity. Because of the complexities of estimating chemical activities and osmolarity, a simplified model based directly on ion concentrations was also developed and found to provide useful predictions. Environ Toxicol Chem 2018;37:247–259. Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
Influence of physicochemical parameters on storage stability: Microbiological quality of fresh unpasteurized fruit juices
Authors: Kaddumukasa Phoebe P. , Imathiu Samuel M. , Mathara Julius M. , Nakavuma Jesca L. 
Published: August 2017

AbstractFresh juices rich in health and nutritional benefits are valued for their fresh flavor, taste, and aroma. These juices' quality however is affected by factors like temperature, light, and microbiological contamination significantly changing physicochemical parameters and storage stability. Physicochemical and microbiological analyses of passion fruit, pineapple, and mango juices in dark and light bottles at 24°C and 4°C were conducted in Kampala, Uganda for 12 days. Physicochemical parameters significantly reduced (p < .05) storage stability of fresh juices, while no significant changes (p > .05) were observed for the microbiological analyses. pH values ranged from 3.0 to 4.2 (dark) bottles and 2.9 to 4.0 (light) bottles for juices at 24°C and 4°C. °Brix values were from 1.0 to 5.5 for dark and clear bottles at 24°C and 4°C. TTA (%) values ranged from 1.1 to 7.2 (dark) bottles and 1.1 to 7.4 for (light) bottles at 24°C and 4°C. Ascorbic acid content ranged from 3.5 to 61.0 mg/ 100 ml and 5.5 to 56.7 mg/100 ml for juices in dark and clear bottles, respectively. total plate counts ranged from 1.3 × 10___ to 3.3 × 107 CFU/ml (dark bottles at 24°C) to 3.5 × 10³ to 3.3 × 108 CFU/ml (dark bottles at 4°C). For juices in light bottles, total plate counts ranged from 1.8 × 10___ to 8.0 × 107 CFU/ml (24°C) and 2.7 × 10___ to 1.5 × 108 CFU/ml (4°C). High microbial loads suggest the use of poor processing techniques and lack of good hygiene which lower quality and reduce storage stability of juices. Storage temperature greatly reduces physicochemical parameters both at ambient and refrigeration temperatures. This implies that temperature control for unpasteurized juices is critical in order to inhibit microorganism metabolic activities which accelerate biodeterioration leading to spoilage and short shelf life. Fresh unpasteurized juices stored at 24°C and 4°C may safely be consumed within 1 and 2 days, respectively.
Effect of processing methods on the nutritional content of three traditional vegetables leaves: Amaranth, black nightshade and jute mallow
Authors: Traoré Korotimi , Parkouda Charles , Savadogo Aly , Ba/Hama Fatoumata , Kamga Regine , Traoré Yves 
Published: August 2017

AbstractThe study assessed changes in nutritional content of some commonly consumed traditional vegetables subjected to postharvest processes. Amaranth (Amaranthus cruentus L.), black nightshade (Solanum scabrum Mill.) and jute mallow (Corchorus olitorius L.) leaves used as vegetables were subjected to blanching, boiling and drying. The proximate composition and β‐carotene content of fresh and processed leaves were determined. Amaranth, black nightshade and jute mallow leaves had 25.21%, 39.74% and 29.18% of protein, respectively. The β‐carotene levels were 16.40, 25.25 and 27.74 mg/100 g for black nightshade amaranth and jute mallow leaves, respectively. The ash content was 10.57% for black nightshade, 12.40% for jute mallow and 16.33% for amaranth. Processing methods caused decreases of β‐carotene and crude lipid content. Boiling for 30 min or more resulted in large loss of β‐carotene. Drying under shade resulted in less loss of β‐carotene than drying in cabinet at 50 and 60°C.
Effect of soy protein isolate on the functional, pasting, and sensory acceptability of cassava starch‐based custard
Authors: Akinwale Toyin E. , Shittu Taofik A. , Adebowale Abdul‐razaq A. , Adewuyi Sheriff , Abass Adebayo B. 
Published: August 2017

AbstractFortification of custard powder (CP) with protein from cheap sources such as soybean could potentially improve its nutritive value but may alter its functional and sensory properties. This study was therefore conducted to determine the effect of soy protein isolate (SPI) inclusion (0%–20%) on some functional and sensory properties of cassava starch‐based CP. Functional, pasting, and sensory acceptability were determined using standard methods. Increase in soy protein isolate significantly (p < .05) decreased dispersibility, packed bulk density, swelling power, peak, trough, breakdown, final, and setback viscosities, but increased least gelation concentration, water absorption capacity, and solubility index. This study further showed that despite increasing addition of SPI up to 20%, sensory acceptability of the cassava starch‐based CP formulations did not differ significantly, and most of them had very similar acceptability when compared to that of corn starch‐based CP.
Effects of cooking techniques on fatty acid and oxylipin content of farmed rainbow trout (Oncorhynchus mykiss)
Authors: Flaskerud Katrina , Bukowski Michael , Golovko Mikhail , Johnson LuAnn , Brose Stephen , Ali Ashrifa , Cleveland Beth , Picklo Matthew , Raatz Susan 
Published: August 2017

AbstractThe aim of this study was to investigate the effect of various cooking techniques on the fatty acid and oxylipin content of farmed rainbow trout. Rainbow trout is an excellent source of long‐chain omega‐3 (n‐3) polyunsaturated fatty acids (PUFA) which have beneficial health effects. Fillets of 2‐year‐old farmed rainbow trout were baked, broiled, microwaved, or pan‐fried in corn (CO), canola (CaO), peanut (PO), or high oleic sunflower oil (HOSO). Fatty acids and oxidized lipids were extracted from these samples and their respective raw fillet samples. Fatty acid content was determined using gas chromatography and oxylipin content by mass spectroscopy. The values obtained from each cooking method were compared to those obtained from the respective raw fillets using paired t tests. PUFA content was not altered when samples were baked, broiled, microwaved, or pan‐fried in CO or CaO. Pan‐frying in PO reduced α‐linolenic acid (18:3n‐3), eicosadienoic acid (20:2n‐6), and dihomo‐γ‐linolenic acid (20:3n‐6), while pan‐frying in HOSO reduced 18:3n‐3, eicosapentaenoic acid (20:5n‐3), docosapentaenoic acid (22:5n‐3), docosahexaenoic acid (22:6n‐3), linoleic acid (18:2n‐6), 18:3n‐6, 20:2n‐6, 20:3n‐6, docosatrienoic acid (22:2n‐6), and adrenic acid (22:4n‐6) compared to raw fish. Cooking decreased the omega‐6 (n‐6) PUFA‐derived oxylipins, but caused no change in 20:5n‐3 or 22:6n‐3‐derived oxylipins of the fillets. In conclusion, pan‐frying was the only cooking method to alter the fatty acid content of the fillets, while observed changes in oxylipin content varied by cooking method. As the physiological impact of oxylipins is currently unknown, these results suggest that the cooking methods which optimize the consumption of n‐3 PUFA from rainbow trout are baking, broiling, microwaving, or pan‐frying in CO, CaO, or PO.
The Genius Dilemma: Fortune 1000 CEO Personality and Firm Innovation
Authors: Gal David 
Published: August 2017

AbstractInnovation is important to organizations’ long‐lasting success, yet many organizations fail to successfully promote innovation. One factor likely to bear an important influence on whether an organization successfully innovates, but that has received relatively little research attention, is the personality of the CEO. The present research examines the link between the personality of the CEO and product and service innovation in the firm using a unique sample of Fortune 1000 CEO's. Results show that conscientiousness in the CEO negatively predicts the level of innovation in the firm. Moreover, this relationship is evident among CEO's who have filled the CEO role for at least 3 years, but not among CEO's who have filled the CEO role for less than 3 years.
GC–MS/MS method for the determination and pharmacokinetic analysis of borneol and muscone in rat after the intravenous administration of Xingnaojing injection
Authors: Song Yunfei , Chu Yang , Ma Xiaohui , Zheng Haoran , Bai Xiaolin , Zhou Shuiping , Yu Boyang 
Published: August 2017

AbstractA simple and sensitive gas chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of borneol and muscone in rat plasma. The analytes and internal standard, naphthalene, were extracted using a convenient one‐step liquid–liquid extraction method with ethyl acetate. The chromatographic separation was realized on a HP‐5MS capillary column and detected in multiple reaction monitoring mode. Excellent linearity (R2 ≥ 0.996) was shown over 10.0–5000 ng/mL for borneol and 2.5–250 ng/mL for muscone. The lower limit of quantitation was 10 and 2.5 ng/mL for borneol and muscone, respectively. The intra‐ and interday precisions were less than 7.52%, and the accuracy values were between −8.03 and 14.52%. The extraction recovery, matrix effect, and stability were sufficient to meet the Food and Drug Administration criteria. Meanwhile, the assay was successfully applied to the preclinical pharmacokinetic study of borneol and muscone following intravenous administration of Xingnaojing injection, a modern Chinese herbal medicine preparation.
Selective swelling blends of block copolymers for nanoporous membranes with enhanced permeability and robustness
Authors: Wang Zhaogen , Liu Rui , Lan Qianqian , Wang Yong 
Published: August 2017

ABSTRACTBlock copolymers (BCPs) are important precursors to produce membranes with well‐defined porosities. However, it remains challenging to prepare robust and affordable BCP‐based membranes. In this work, cheap commodity styrene‐butadiene‐styrene (SBS) elastic triblock copolymers are mixed with polystyrene‐block‐poly (2‐vinylpyridine) (SV) block copolymers in solutions, leading to macroscopically stable blend films upon casting because of the compatibilizer effect of PS existing in both copolymers. By soaking the blend films in ethanol, the microdomains of poly(2‐vinylpyridine) are selectively swollen and cavitated upon drying, resulting in a hierarchical structure with perforated SV phases interwoven with the SBS phases. The blend membranes with 30% SBS exhibit improved water permeability and mechanical robustness due to the presence of elastic SBS compared to neat SV membranes; meanwhile, the rejections of the blend membranes remain largely unchanged. Moreover, the blend membranes exhibit a pH‐responsive function, and homoporous SV regions are obtained by pre‐aligning the SV phases. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2017, 55, 1617–1625
Water‐soluble top coats for orientation control of liquid crystal‐containing block copolymer films
Authors: Xie He‐Lou , Li Xiao , Suh Hyo Seon , Ren Jia‐Xing , Wan Ling‐Shu , Craig Gordon S. W. , Arges Christopher G. , Nealey Paul F. 
Published: August 2017

ABSTRACTAn easily removable, water‐soluble top coat of polyvinylpyrrolidone (PVP) is used to control the orientation of microdomains in a liquid crystalline block copolymer (LC BCP, poly(ethylene oxide)‐block‐poly(6‐(4‐methoxy‐azobenzene‐4′‐butyl) hexyl methacrylate)). The corresponding LC homopolymer is also investigated for comparison. Atomic force microscopy is used to determine the orientation of the cylindrical microdomains of the LC BCP. UV–vis spectroscopy and grazing incidence wide‐angle X‐ray scattering are used to determine the orientation of the LC mesogens in the LC homopolymer and the LC BCP films annealed both with and without a top coat. Once the LC BCP morphology is self‐assembled, the PVP top coat layer can be easily removed with water or alcohol. The facile removal of the top coat improves the processability of BCPs in technological applications, and enables direct investigation of the BCP morphology in scientific studies. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2017, 55, 1569–1574
A large‐scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand‐binding domains of taste receptor
Authors: Yamashita Atsuko , Nango Eriko , Ashikawa Yuji 
Published: August 2017

AbstractMany of the extracellular proteins or extracellular domains of plasma membrane proteins exist or function as homo‐ or heteromeric multimer protein complexes. Successful recombinant production of such proteins is often achieved by co‐expression of the components using eukaryotic cells via the secretory pathway. Here we report a strategy addressing large‐scale expression of hetero‐multimeric extracellular domains of plasma membrane proteins and its application to the extracellular domains of a taste receptor. The target receptor consists of a heterodimer of T1r2 and T1r3 proteins, and their extracellular ligand binding domains (LBDs) are responsible for the perception of major taste substances. However, despite the functional importance, recombinant production of the heterodimeric proteins has so far been unsuccessful. We achieved the successful preparation of the heterodimeric LBD by use of Drosophila S2 cells, which have a high secretory capacity, and by the establishment of a stable high‐expression clone producing both subunits at a comparable level. The method overcame the problems encountered in the conventional transient expression of the receptor protein in insect cells using baculovirus or vector lipofection, which failed in the proper heterodimer production because of the biased expression of T1r3LBD over T1r2LBD. The large‐scale expression methodology reported here may serve as one of the considerable strategies for the preparation of multimeric extracellular protein complexes.