bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA sequencing comparing centenarian and middle‐aged women lymphoblastoid cell lines identifies age‐related dysregulated expression of genes encoding selenoproteins, heat shock proteins, CD99, and BID
Authors: Voinsky Irena , Goldenberg‐Bogner Ofir , Israel‐Elgali Ifat , Volkov Hadas , Puzianowska‐Kuźnicka Monika , Shomron Noam , Gurwitz David 
Published: October 2024

AbstractWomen typically live longer than men, and constitute the majority of centenarians. We applied RNA‐sequencing (RNA‐seq) of blood‐derived lymphoblastoid cell lines (LCLs) from women aged 60‐80 years and centenarians (100‐105 years), validated the RNA‐seq findings by real‐time PCR, and additionally measured the differentially expressed genes in LCLs from young women aged 20‐35 years. Top RNA‐seq genes with differential expression between the age groups included three selenoproteins (GPX1, SELENOW, SELENOH) and three heat shock proteins (HSPA6, HSPA1A, HSPA1B), with the highest expression in LCLs from young women, indicating that young women are better protected from oxidative stress. The expression of two additional genes, BID encoding BH3‐interacting domain death agonist and CD99 encoding CD99 antigen, showed unique age dependence, with similar expression levels in young and centenarian women while exhibiting higher and lower expression levels, respectively, in LCLs from women aged 60‐80 years compared with the two other age groups. This age‐related differential expression of BID and CD99 suggests elevated inflammation susceptibility in middle‐aged women compared with either young or centenarian women. Our findings, once validated with human peripheral blood mononuclear cells and further cell types, may lead to novel healthy aging diagnostics and therapeutics.
Clinical usefulness of hybrid endoscopic submucosal dissection for T1b colorectal carcinomas ≤20 mm to ensure adequate vertical margins
Authors: Takehara Yudai , Yamashita Ken , Morimoto Shin , Tanino Fumiaki , Yamamoto Noriko , Kamigaichi Yuki , Tanaka Hidenori , Takigawa Hidehiko , Urabe Yuji , Kuwai Toshio , Arihiro Koji , Oka Shiro 
Published: October 2024

AbstractObjectiveTo evaluate endoscopic resection strategies for cT1b colorectal carcinomas (CRCs) ≤20 mm to determine strategies that enable adequate vertical margins (VMs).MethodsWe enrolled 128 consecutive patients with cT1b colorectal carcinomas ≤20 mm resected by endoscopic mucosal resection or hybrid endoscopic submucosal dissection (ESD). Tumor lifting conditions after submucosal injection were classified into type A (lifting, soft dome‐like), type B (lifting, hard trapezoid‐like), and non‐lifting (positive non‐lifting sign). Predictors of positive VMs (VM 1) and adequate VMs were identified.ResultsAll non‐lifting tumors were resected by hybrid ESD and VMs were ≥500 µm. Vertical margin 1 tumors were only found in the endoscopic mucosal resection group, in which, the proportion of type B tumors with VM 1 was significantly higher than that of tumors with negative VMs (p < 0.01). Type A tumors showed no significant between‐group differences. Among type B tumors, the proportion of VMs ≥500 µm was significantly higher (p < 0.01) and the VM distance was significantly longer (p < 0.01) in the hybrid ESD group than in the endoscopic mucosal resection group.ConclusionsHybrid ESD can be selected for type B tumors to ensure adequate VMs.
CD24 regulates liver immune response and ameliorates acute hepatic injury through controlling hepatic macrophages
Authors: Zheng Jian , Xiao Jun , Fan Yatong , Zheng Honggang , Liu Hongyu , Xiang Jie , Hai Lei , Wang Yan , Zhang Xuejun 
Published: October 2024

AbstractLiver injury releases danger‐associated molecular patterns, which trigger the immune response. CD24 negatively regulates the immune response by binding with danger‐associated molecular patterns, but the specific role of CD24 in modulating macrophage‐related inflammation during liver injury remains largely unexplored. Here, we aimed to investigate the mechanisms of macrophage CD24 in the development of liver injury. Our results show that CD24 expression is upregulated primarily in hepatic macrophages (HMs) during acute liver injury. CD24‐deficient mice exhibited more severe liver injury and showed a significantly higher frequency and number of HMs, particularly Ly6Chi monocyte‐derived macrophages. Mechanistically, the CD24‐Siglec‐G interaction plays a vital role in mitigating acute liver injury. CD24‐mediated inhibitory signaling in HMs primarily limits downstream NF‐κB and p38 MAPK activation through the recruitment of SHP1. Our work unveils the critical role of macrophage CD24 in negatively regulating innate immune responses and protecting against acute liver injury, thus providing potential therapeutic targets for liver‐associated diseases.
High–Field EPR Studies on Three Binuclear μ–Oxo–Bridged Iron(III) Complexes: Na4[Fe(edta)]2O ⋅ 3H2O, [Fe(phen)2(H2O)]2O(NO3)4 ⋅ 5H2O and [Fe(salen)]2O
Authors: Ozarowski Andrew 
Published: October 2024

AbstractA High–Field, High–Frequency Electron Paramagnetic Resonance (HFEPR) study was performed on the binuclear μ–oxo bridged complexes Na4[Fe(edta)]2O ⋅ 3H2O, [Fe(phen)2(H2O)]2O(NO3)4 ⋅ 5H2O and [Fe(salen)]2O, where edtaH4 = ethylenediaminetetraacetic acid, phen=1,10–phenanthroline and salenH2 is NN′–ethylenebis(salicylideneimine). The spin Hamiltonian parameters were determined for the thermally excited spin states with spin S=1, 2 and 3. The contributions to the zero–field splitting due to the local zero field splitting on individual iron ions and to the anisotropic metal–metal interactions were evaluated. Surprisingly large magnitudes of the anisotropic exchange interactions were found.
Nickel Bis(dithiolene) Complexes and Tetrathiafulvalenes from Sterically Hindered Di‐ and Tetra‐Substituted Dithiine‐dithiolo‐thione Precursors
Authors: Mroweh Nabil , Abhervé Alexandre , Auban‐Senzier Pascale , Pop Flavia , Avarvari Narcis 
Published: October 2024

AbstractThree variants of 5,6‐dihydro‐1,4‐dithiin‐2,3‐(1’,3’‐dithiole‐2’‐thione) (dddt) bearing up to four substituents on the ethylene bridge have been obtained by hetero‐Diels‐Alder reaction. The substituted dddt derivatives have the advantage of providing modulation of the steric hindrance and stereogenic centres, and represent valuable intermediates for both tetrathiafulvalene (TTF) and metal‐dithiolene complexes. Within this work, disubstituted and tetrasubstituted dddt derivatives have been used for the synthesis of Ni(II) monoanionic and neutral complexes, as well as for tetra‐ and octa‐methylated EDT‐TTF and BEDT‐TTF (EDT=ethylenedithio, BEDT=bis(ethylenedithio)) derivatives. While the influence of the bulkiness and number of the substituents is observed in the solid state packing of both types of materials (complexes and TTF), the absence of the counterion in the case of the neutral complexes translated into increased intermolecular interactions between the donors and the formation of 1D and 2D layers. The two examples of tetrasubstituted TTF radical cation salts reported in here show semiconducting behaviour due to the formation of dimers and lateral interactions through the S atoms. Our findings highlight the importance of substitution on these type of dddt precursors and their potential for electroactive molecular materials.
Individuals From Higher Social Classes View Charitable Giving as a Solution to Societal Issues
Authors: Klebl Christoph , Chertavian Callum , Jetten Jolanda 
Published: October 2024

ABSTRACTCharitable giving is often seen as a flexible and efficient way to address societal issues. However, it has also been criticised for having the potential to undermine governmental responsibility for providing public services and leading to concentrations of power. Across three studies, we investigated whether high social class is linked to viewing charitable giving favourably and as fulfilling an important function in society. We found correlational (Studies 1 and 2; United Kingdom and Netherlands; N = 1613) and experimental (Study 3; United Kingdom; N = 400) evidence that high social class is linked to more favourable views of charitable giving. Social class, however, was not associated with preferences for policies aimed at promoting charitable giving over systemic policies when targeting specific social issues. These findings suggest that social class influences general attitudes towards philanthropy but does not consistently guide preferences for specific policies addressing social issues.
Comparison of MIB‐1‐Specific Membrane Staining in Hyalinising Trabecular Tumor Using Mainstream Automated Immunohistochemical Staining Platforms
Authors: Hong Bo , Xu Yanfei , Xiao Yufei , Yu Xiaoyan 
Published: October 2024

ABSTRACTBackgroundMIB‐1, a monoclonal antibody against Ki‐67, exhibits specific membrane staining in the immunohistochemistry of hyalinising trabecular tumor (HTT). This specific staining pattern is crucial in diagnosing HTT. Although manual immunohistochemical staining remains the established method for MIB‐1 staining, this process is complicated, inconsistent, and prone to false negatives.MethodsThis study aimed to explore whether the classical reaction pattern can be replicated by utilizing the current mainstream automated immunohistochemical staining platforms. Furthermore, we examined the effect of different conditions on staining efficiency and their value in clinical diagnosis assistance.ResultsSpecimens obtained from eight and six cases of HTT and non‐HTT, respectively, from a single center were stained using the manual staining method and the Dako Autostainer Link 48 (AS48), Dako Omnis, Ventana BenchMark ULTRA, and Leica BOND‐III automated immunohistochemical staining platforms. The Autostainer Link 48 was found to be the most stable staining platform, while the BenchMark ULTRA with primary antibody incubation at room temperature (RT) and the Omnis platform with antigen retrieval at pH 9.0 were able to reproduce membrane‐positive staining for MIB‐1 in the HTT specimens.ConclusionsOur results offer crucial reference value for clinical diagnostic assistance.
Role of hsa‐miR‐543‐KIF5C/CALM3 pathway in neuron differentiation of embryonic mesenchymal stem cells
Authors: An Dongmei , Wang Yangfan , Wang Xin 
Published: October 2024

AbstractBackgroundHuman umbilical cord mesenchymal stem cells (hUC‐MSCs) have the ability to differentiate into nerve cells, which offers promising options for treating neurodegenerative diseases.AimTo explore the important regulatory molecules of hUC‐MSCs differentiation into neurons.MethodIn this research, the neural differentiation of hUC‐MSCs was induced by a low‐serum DMSO/BHA/DMEM medium. The GEO database was used to retrieve the relevant datasets. The starBase and miEAA databases were used for bioinformatics analysis. RT‐qPCR was used to detect the hsa‐miR‐543 level and the mRNA levels of NSE, NeuN, NF‐M, KIF5C, and CALM3. The protein levels of KIF5C and CALM3 were checked by western blotting.ResultsThe expression levels of NSE, NeuN, NF‐M, KIF5C, and CALM3 were elevated, while hsa‐miR‐543 was under‐expressed in neuro‐induced hUC‐MSCs. The increase in NSE, NeuN, and NF‐M mRNA levels induced by DMSO/BHA/DMEM was partially reversed by the knockdown of KIF5C and CALM3 in hUC‐MSCs. Moreover, the transfection of hsa‐miR‐543 mimic partially countered the DMSO/BHA/DMEM‐induced elevation in NSE, NeuN, NF‐M, KIF5C, and CALM3 mRNA levels.ConclusionKIF5C and CALM3 facilitated the neuronal differentiation of hUC‐MSCs, whereas hsa‐miR‐543 exerted an opposing effect by negatively regulating KIF5C and CALM3.
Advancing Native Mass Spectrometry Toward Cellular Biology
Authors: Saikusa Kazumi 
Published: October 2024

ABSTRACTProtein structure, including various post‐translational modifications and higher‐order structures, regulates diverse biological functions. Native mass spectrometry (native MS) is a powerful analytical technique used to determine the masses of biomolecules, such as proteins and their complexes, while preserving their native folding in solution. This method provides structural information on the composition of monomers or complexes and the stoichiometry of subunits within each complex, significantly contributing to protein structural analysis. Native MS has evolved to incorporate top–down approaches, enabling the characterization of proteoforms and non‐covalent interactions between metabolites or proteins and specific targets. This perspective highlights the advancements in native MS for intracellular proteins and protein complexes, and discusses future research directions toward cellular biology.
Concurrent Inter‐Model Spread of Boreal Winter Westerly Jet Meridional Positions Between the Northern and Southern Hemispheres in CMIP6 Models
Authors: Tang Li , Lu Riyu , Lin Zhongda , Lu Jian , Chen Ziming 
Published: October 2024

ABSTRACTThis study investigates the inter‐model spread of climatological extratropical westerly jets in boreal winter, using the historical simulation of 52 Coupled Model Intercomparison Project phase 6 (CMIP6) models from 1851 to 2014. The results show that there is a substantial spread in the latitude of the upper‐tropospheric westerly jet across models, characterised by large inter‐model standard deviations to both the poleward and equatorward sides of the jet axis, although the multi‐model ensemble mean (MME) performs well in simulating meridional position of westerly jets. Furthermore, we detect the consistency of inter‐model jet position spread between the Northern and Southern Hemispheres, based on the inter‐model empirical orthogonal function (EOF) decomposition and correlation of regional‐averaged zonal winds. Specifically, the models that simulate the westerly jets poleward/equatorward relative to the MME position in one hemisphere also tend to simulate the jets poleward/equatorward in the other hemisphere. Accordingly, we define a global jet spread index to depict the concurrence of jet shift in the two hemispheres. The results of inter‐model regression analyses based on this index indicate that the models positioning the jets poleward than the MME tend to simulate a wider Hadley Cell, a poleward‐shifted Ferrel Cell in the Southern Hemisphere, enhanced precipitation in the subtropics and suppressed precipitation in the tropics, and warmer sea surface temperatures in the subtropics and mid‐latitudes. The present results suggest that improving the simulation of jet positions in climate models requires a comprehensive consideration of thermal states in the tropics and subtropics/mid latitudes.
Exploring methodological approaches used in network meta‐analysis of psychological interventions: A scoping review
Authors: Boonpattharatthiti Kansak , Ruenin Garin , Kulwong Pun , Lueawattanasakul Jitsupa , Saechao Chintra , Pitak Panitan , Caldwell Deborah M. , Chaiyakunapruk Nathorn , Dhippayom Teerapon 
Published: October 2024

AbstractPsychological interventions are complex in nature and have been shown to benefit various clinical outcomes. Gaining insight into current practices would help identify specific aspects that need improvement to enhance the quality of network meta‐analysis (NMA) in this field. This scoping review aimed to explore methodological approaches in the NMA of psychological interventions. We searched PubMed, EMBASE, and Cochrane CENTRAL in September 2023. We included NMAs of psychological interventions of randomized controlled trials that reported clinical outcomes. Three independent researchers assessed the eligibility and extracted relevant data. The findings were presented using descriptive statistics. Of the 1827 articles identified, 187 studies were included. Prior protocol registration was reported in 130 studies (69.5%). Forty‐six studies (24.6%) attempted to search for gray literature. Ninety‐four studies (50.3%) explicitly assessed transitivity. Nearly three‐quarters (143 studies, 76.5%) classified treatment nodes by the type of psychological intervention, while 13 studies (7.0%) did so by lumping different intervention types into more broader intervention classes. Seven studies (3.7%) examined active components of the intervention using component NMA. Only three studies (1.6%) classified interventions based on factors affecting intervention practices, specifically intensity, provider, and delivery platform. Meanwhile, 29 studies (15.5%) explored the influential effects of these factors using meta‐regression, subgroup analysis, or sensitivity analysis. The certainty of evidence was assessed in 80 studies (42.8%). The methodological approach in NMAs of psychological interventions should be improved, specifically in classifying psychological interventions into treatment nodes, exploring the effects of intervention‐related factors, and assessing the certainty of evidence.
Prescribing patterns amongst UK dermatologists for the treatment of alopecia areata, female pattern hair loss, and frontal fibrosing alopecia
Authors: Frewen John , Alsaadi Dalia , Asfour Leila , Belmo Sharon , Bryden Alyson , Champagne Caroline , Clayton Nicola , Cooke Nicola , Cummins Donna M. , Fairhurst David , Farrant Paul , Hale Gordan , Holmes Susan , Harries Thomas , Jones Ruth , Karanovic Sanja , Kaur Manjit R. , Meah Nekma , Mowbray Megan , Rao Archana , Rouhani Nasim , Salmon Nicola , Takwale Anita , Wade Martin , Wong Sharon , Zaheri Shirin , Al‐Nuaimi Yusur , Harries Matthew 
Published: October 2024

AbstractBackgroundTherapeutic management of hair loss is frequently complicated by a lack of high‐quality evidence and reliant on the use of unlicensed therapies. Treatment decision‐making is predominantly based on expert opinion, local availability, personal experience, and cost, which make informed choices challenging for clinicians and patients in this area.ObjectivesThe aims were to determine prescribing patterns amongst UK Dermatologists with a special interest in hair disorders, when treating mild‐moderate alopecia areata (AA), severe AA (including alopecia totalis/alopecia universalis), female pattern hair loss (FPHL) and frontal fibrosing alopecia (FFA).MethodsConsultant members of the British Hair and Nail society, a special interest group affiliated to the British Association of Dermatologists, were invited to participate from across the United Kingdom. Participants were questioned on their current prescribing patterns in both NHS and private practice, were asked to rank their first‐to‐fifth line treatments for each condition and highlight the treatment they perceive as most effective for each disorder.ResultsTwenty‐six Consultant Dermatologists completed the questionnaire, from twenty‐three institutions. For treatment of mild‐moderate AA, topical corticosteroids were used first line amongst 65% (n = 17) of respondents, and 82% (n = 23) reported that intralesional corticosteroids were the most effective treatment. For severe AA, oral corticosteroids were used first line amongst 38% (n = 10) of respondents, and 25% (n = 8) reported that oral corticosteroids were the most effective treatment. For FPHL, topical minoxidil was used first line amongst 84% (n = 25) of respondents, and 42% (n = 10) reported that oral minoxidil was the most effective treatment. For FFA, topical corticosteroids were used first line amongst 62% (n = 16) of respondents, and 37% (n = 14) reported that hydroxychloroquine was the most effective treatment.ConclusionsThis study reports real‐world prescribing practices amongst dermatologists treating common hair loss conditions. These results aim to support clinicians with decision making for managing hair loss conditions.
Snow removal cools a small dystrophic lake
Authors: Dugan H. A. , Ladwig R. , Schramm P. , Lottig N. R. 
Published: October 2024

AbstractLimnological understanding of the role snow plays in under‐ice thermal dynamics is mainly based on studies of clear‐water lakes. Very little is known about the role snow plays in the thermal dynamics of dystrophic lakes. We conducted a whole lake experiment on a small, 8 m deep dystrophic bog lake in northern Wisconsin, where we removed all snowfall over two consecutive winters. Due to weather variability, only 1 year had predominantly black ice. Under these conditions, the lake rapidly cooled in early and mid‐winter, compared to snow covered conditions that insulated the lake from heat loss. The lake also rapidly gained heat in late winter resulting in isothermal conditions well in advance of ice‐off. These results show how water clarity modulates the influence of snow on under‐ice thermal dynamics, which is relevant to futures with snow droughts.
Inverse‐Designed Broadband Ultrathin Aperiodic Metacoatings Spanning from Deep Ultraviolet to Near Infrared
Authors: Chen Zhengai , Wen Zhengji , Wu Zhixuan , Gao Yanqing , Tan Chong , Deng Zhixiang , Li Xiaowen , Zhou Dongjie , Liu Feng , Hao Jiaming , Huang Zhiming , Dai Ning , Sun Yan 
Published: October 2024

AbstractThe ability to actively control broadband light absorption and reflection ranging from deep‐ultraviolet (DUV) to near‐infrared (NIR), is crucial for various optical and optoelectronic applications. Herein, a novel type of ultrathin, lithography‐free, and broadband aperiodic thin‐film metamaterial coatings (metacoatings) is inverse‐designed from DUV to NIR (190–1700 nm), which is composed of a series of deeply subwavelength tungsten oxide layers with graded refractive index profiles (by varying the deposited Ar gas pressures from 0.1 to 3.0 Pa) above an opaque aluminum layer. Experimental results show that these proposed aperiodic metacoatings can realize a transition from broadband strong absorption (as‐deposited state) to high reflection (annealed state) from visible (VIS) to NIR (400–1700 nm), through thermal annealing techniques. The aperiodic metacoatings have an overall thickness of just ≈200 nm, with angle‐insensitive up to 70° as well as are polarization‐independent. Furthermore, vivid structural colors can be generated by rearranging the aperiodic tungsten oxide layers and finally employed in color display applications. These aperiodic metacoatings offer a very promising platform for many applications such as broadband photodetectors, thermophotovoltaics, information encryption devices, etc.
Detailed Compositional and Structure–Property Analysis of Ethylene Oxide‐Propylene Oxide Triblock Copolymers
Authors: Róth Gergő , Nagy Tibor , Kuki Ákos , Novák Levente , Nyul Dávid , Zsuga Miklós , Kéki Sándor 
Published: October 2024

AbstractThe polyethylene oxide‐polypropylene oxide (PEO‐PPO) based triblock copolymers are notable amphiphilic copolymers with a diverse range of applications. The presence of homo‐, and/or diblock impurities in copolymers with PEO‐PPO‐PEO triblock has been demonstrated. This finding suggests that the samples are blends rather than pure triblock copolymers. Furthermore, copolymers with triblock copolymer content of 0 or less than 20% by molar percentage have been identified. The hydrophilic‐lipophilic balance (HLB) is also calculated based on the exact composition of the blends. The effect of HLB values and compositional data on the initial foam height (in casein solution), the surface tension, and the contact angle are investigated. The correlation coefficients for the PPO‐PEO‐PPO copolymers versus HLB values are found to be high, while those obtained for the PEO‐PPO‐PEO copolymers versus values of HLB are significantly lower. The lower correlation coefficients for the PEO‐PPO‐PEO samples can be attributed to the presence of homo‐ and diblock (co)polymer contaminants. In addition, a linear regression model has been constructed to find a mathematical relationship between the percentage of ethylene oxide, the average number of propylene oxide units, and the properties of the copolymer.