bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hippo kinases maintain polarity during directional cell migration in Caenorhabditis elegans
Authors: Feng Guoxin , Zhu Zhiwen , Li Wen‐Jun , Lin Qirong , Chai Yongping , Dong Meng‐Qiu , Ou Guangshuo 
Published: December 2016

AbstractPrecise positioning of cells is crucial for metazoan development. Despite immense progress in the elucidation of the attractive cues of cell migration, the repulsive mechanisms that prevent the formation of secondary leading edges remain less investigated. Here, we demonstrate that Caenorhabditis elegans Hippo kinases promote cell migration along the anterior–posterior body axis via the inhibition of dorsal–ventral (DV) migration. Ectopic DV polarization was also demonstrated in gain‐of‐function mutant animals for C. elegans RhoG MIG‐2. We identified serine 139 of MIG‐2 as a novel conserved Hippo kinase phosphorylation site and demonstrated that purified Hippo kinases directly phosphorylate MIG‐2S139. Live imaging analysis of genome‐edited animals indicates that MIG‐2S139 phosphorylation impedes actin assembly in migrating cells. Intriguingly, Hippo kinases are excluded from the leading edge in wild‐type cells, while MIG‐2 loss induces uniform distribution of Hippo kinases. We provide evidence that Hippo kinases inhibit RhoG activity locally and are in turn restricted to the cell body by RhoG‐mediated polarization. Therefore, we propose that the Hippo–RhoG feedback regulation maintains cell polarity during directional cell motility.
Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw
Authors: Supriyatna Ateng , Ukit Ukit 
Published: December 2016

<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., &amp; Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 314-320. </p>
Confirmation of Tuntun Angin (Elaeocarpus floribundus) Taxonomic Status Using matK and ITS Sequences
Authors: Roslim Dewi Indriyani , Khumairoh Siti , Herman Herman 
Published: December 2016

<p>Tuntun angin is one of important floodplain plants in and around Kajuik Lake located in Riau Province, Indonesia. Morphological identification shows that the scientific name of this plant is <em>Elaeocarpus floribundus. </em>The study aimed to confirm the taxonomic status of tuntun angin using <em>matK</em> and nuclear intergenic spacer (ITS) sequences. The methods included fresh leaf DNA isolation, polymerase chain reaction, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.06 programs. The results showed that the <em>matK</em> sequence (519 bp) of tuntun angin had highest similarity to <em>E. floribundus</em> <em>matK</em> sequence that was available in GenBank. It was supported by the high max score (937), low E-value (0.0), high identity value (100%), and high query cover (100%). However, the ITS sequence of tuntun angin did not show similarity to <em>E. floribundus </em>ITS sequence because there was no database of the sequence in GenBank. This study was able to confirm the taxonomic status of tuntun angin as <em>E. floribundus</em> using <em>matK</em> sequence and also showed that morphological and molecular identification techniques were complementary to each other. Moreover, this study enriched the DNA sequence database of <em>E. floribundus </em>in GenBank which will be useful for this species’ molecular identification.</p><p><strong>How to Cite</strong></p><p>Roslim, D. I., Khumairoh, S. &amp; Herman, H. (2016). Confirmation of Tuntun Angin (<em>Elaeocarpus floribundus</em>) Taxonomic Status Using <em>matK</em> and ITS Sequences. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 393-400. </p>
UM ENFOQUE TRANSDISCIPLINAR PARA ANÁLISE DA COMPLEXIDADE DO DIREITO À SAÚDE
Authors: Szinvelski Mártin Marks , Martini Sandra Regina 
Published: December 2016

O processo de evolução social impacta na estrutura de sistemas sociais, seja porque os novos direitos complexificam o processo, seja porque a função de redução da complexidade, inerente à função de cada sistema, incrementa a própira complexidade. Sabe-se que o campo do direito à saúde é fértil para a análise do duplo jogo de redução e incremento da complexidade. Nesse artigo, essa análise se fundamenta numa visão que ultrapassa os limites de uma única ciência. Portanto, se analisará o Sistema Social da Saúde, tendo em vista o impacto da transdisciplinaridade na efetivação do direito à saúde.
PROFILE OF COGNITIVE STRUCTURE OF STUDENTS IN UNDERSTANDING THE CONCEPT OF REAL ANALYSIS
Authors: Widada Wahyu 
Published: December 2016

The purpose of this research is to describe proil cognitive structure of students in understanding the concept of real analysis. This research is part of the research development of the theory of cognitive structure of students Mathematics Education Program at the University of Bengkulu. The results of this research are: 1)there are seven models decompositions of genetic students mathematics education reviewed based on the SRP Model about the concepts of Real Analysis namely Pra-Intra Level, Level intra, Level semi-inter, Level inter, Level semi-trans, Trans Level, level and Extended-Trans (only theoretic level while empirically not found); 2) There are six models decompositions of genetic students mathematics education reviewed based on KA about the concepts of Real Analysis namely Level 0: Objects of concrete steps; Level 1: Models Semi-concrete steps; Level 2: Models Theoretic; Level 3: Language in Domain Example; Level 4: Mathematical Language; Level 5: Inferensi Model. Profile of cognitive structure of mathematics education student at the University of Bengkulu is 6.25% Students located on the Basic Level (Pra-Intra Level with concrete objects), there is 8.75% Students located at Level 0 (intra Level with concrete objects), there are 15,00% Students located at Level 1 (semi-Level inter with Semi-Concrete Model), there are 33.75 percent students located on Level 2 (Level inter with theoretical model), there are 22.50 percent students located at Level 3 (Semi-trans Level with the Bible in Domain example), there are located on the student percent during the Level 4 (Trans Level with the language of Mathematics), and there are 0 percent students located at Level 5 (Level Extended-Trans with Inferensi Model). Students Education Mathematics at the University of Bengkulu pembangunnya element is functional can achieve Trans Level, students will be able to set up activities and make the algorithm that formed the concept/principles with the right. Functional students can also perform the process of abstraction using the rules in a system of mathematics.
La Révolution tranquille: Rupture ou Tournant?
Authors: Rouillard Jacques 
Published: December 2016

La Révolution tranquille représente un événement central dans l’histoire du Québec. L’interprétation traditionnelle, qui imprègne encore la mémoire collective des Québécois et qui se retrouve dans plusieurs travaux, dépeint le début des années 1960 comme un moment de rupture pour la société francophone après une longue période de conservatisme. Elle marquerait leur entrée dans le monde moderne et l’adoption de valeurs et d’orientations mieux en accord avec une société industrielle. Cette vision du passé est remise en cause depuis un peu plus d’une vingtaine d’années par un groupe d’historiens qui interprètent cet événement moins en terme de rupture que comme un tournant de son histoire. Loin d’être une société immobile, le Québec francophone se transformerait depuis longtemps, notamment sous le coup de l’industrialisation et de l’urbanisation. Notre propos dans ce texte est de réaffirmer la pertinence de cette dernière interprétation pour comprendre le Québec contemporain à un moment où certains historiens la mettent en doutent Nous y regroupons les conclusions de nombreux travaux et présentons des données nouvelles pour mettre en relief les similitudes de développement du Québec avec les autres sociétés nord-américaines et montrer la diversité des forces qui façonnent son histoire. Nous nous attachons d’abord à démontrer dans quelle mesure l’industrialisation et l’urbanisation ont touché la société francophone. Puis, nous mettons en relief l’existence d’une bourgeoisie d’affaires dynamique et d’une classe ouvrière vigoureuse. Enfin, nous identifions les forces sociales qui diffusent la pensée libérale en milieu francophone, faisant ainsi contrepoids aux groupes clérico-conservateurs.
With an Ear to the Ground: The CCF/NDP and Aboriginal Policy in Canada, 1926-1993
Authors: Tester Frank James , McNicoll Paule , Forsyth Jessie 
Published: December 2016

The paper examines the historical role of the CCF/NDP in the making of federal Aborigina policy in Canada. It commences with an examination of the importance of the CCF/NDP rolt in influencing federal policy. This is followed by a critical examination of the prevailing attitudes and values held by the party's founders — including its first leader — J.S. Woodsworth It examines significant changes subsequently made to the Indian Act, culminating with the legislation of 1951, the party's response to the 1969 white paper, revisions to the Canada Act (1982) and subsequent attempts at amendment — the failed Meech Lake Accord (1987) and the Charlottetown Accord of 1992. The party's position on key issues affecting Aboriginal peoples has often been contradictory and, particularly in the case of deliberations about the Canada Act (1981) have created rifts within caucus. What emerges, however, is evidence that the party, despite not always adopting the most progressive stance with respect to Aboriginal aspirations, has, con sistent with its populist roots, been willing to listen and to modify its position in accord with the emerging demands of Aboriginal peoples. The details of this process reveal the roles of populist leanings and ideological principles in shaping party policy.
Northrop Frye: Liberal Humanism and the Critique of Ideology
Authors: Long Douglas 
Published: December 2016

Despite Northrop Frye's expressed desire to contribute to the discussion, within a very broad constituency of educated citizens, of fundamental socio-political issues such as societal cohesion amid cultural diversity, the validation of structures of power and the idea of progress, his reflections have received scant attention from social scientists. After briefly illustrating some of Frye's political concerns and insights, this essay locates in his taxonomy of verbal structures, deployed most importantly in Words with Power (1990), the basis for an incisive critique of the modalities of political discourse. It concentrates specifically on his distinction between the divisive and iniquitous rhetoric of ideology, expressive of the human urge for domination and advantage, and the inclusive and unifying language of myth, expressive of what Frye calls “primary concerns.”
Seeking an expanded, multidimensional conceptual approach to health literacy and health disparities research
Authors: Logan Robert A. 
Published: December 2016

This chapter compares the conceptual foundations of health literacy and health disparities. It details some of the conceptual differences between health literacy and health disparities and explains some similarities that suggest the need for increased research collaboration. The chapter is among the first to address the structural and social determinants of health together and explain that future research needs to assess their interaction. Overall, the chapter creates a conceptual foundation as well as challenges future scholars/practitioners to take more multidimensional approaches to assess health’s determinants. The chapter also attempts to demonstrate there is nothing more practical than good theory, or clear conceptual foundations. The chapter is divided into four sections that address the following topics: three conceptual frameworks about the determinants of health; opportunities in health disparities and health literacy research; seeking an expanded, multidimensional conceptual approach to health literacy and health disparities research; as well as a conclusion. The chapter suggests there are vacuums in current research knowledge that need future attention – especially regarding the integration of health literacy and health disparities research.
Research data centre services for complex software environments in the humanities
Authors: Bingert Sven , Buddenbohm Stefan 
Published: December 2016

Research data is – regardless its disciplinary provenance – very heterogeneous in terms of data formats, applied research methods and content. In contrast to publications, a much broader spectrum of data representations has to be considered by research data infrastructures. Within the Humanities Data Centre project (HDC) an initial service portfolio for research data from the humanities has been developed in its design phase (2014–2016). Not only does this service portfolio has to facilitate the re-use of research data by third parties, a service anybody would expect as conventional for a research data centre, but it also has to provide novel benefits for the providers of research data to promote their contribution of data, documentation and support. As an example for these intended benefits for research data providers we will introduce two service components: the application preservation and the referencing of complex software environments. Jointly applied they enable researchers to archive and reference to complex representations of research data such as digital editions, virtual research environments, or data visualisations.
The bi-objective workflow satisfiability problem and workflow resiliency
Authors: Crampton Jason , Gutin Gregory , Karapetyan Daniel , Watrigant Rémi 
Published: December 2016

A computerized workflow management system may enforce a security policy, specified in terms of authorized actions and constraints, thereby restricting which users can perform particular steps in a workflow. The existence of a security policy may mean that a workflow is unsatisfiable, in the sense that it is impossible to find a valid plan (an assignment of steps to authorized users such that all constraints are satisfied). Work in the literature focuses on the workflow satisfiability problem, a decision problem that outputs a valid plan if the instance is satisfiable (and a negative result otherwise). In this paper, we introduce the Bi-Objective Workflow Satisfiability Problem (BO-WSP), which enables us to solve optimization problems related to workflows and security policies. In particular, we are able to compute a “least bad” plan when some components of the security policy may be violated. In general, BO-WSP is intractable from both the classical and parameterized complexity point of view (where the parameter is the number of steps). We prove that computing a Pareto front for BO-WSP is fixed-parameter tractable (FPT) if we restrict our attention to user-independent constraints. This result has important practical consequences, since most constraints of practical interest in the literature are user-independent. Our proof is constructive and defines an algorithm, the implementation of which we describe and evaluate. We also present a second algorithm to compute a Pareto front which solves multiples instances of a related problem using mixed integer programming (MIP). We compare the performance of both our algorithms on synthetic instances, and show that the FPT algorithm outperforms the MIP-based one by several orders of magnitude on most instances. Finally, we study the important question of workflow resiliency and prove new results establishing that known decision problems are fixed-parameter tractable when restricted to user-independent constraints. We then propose a new way of modeling the availability of users and demonstrate that many questions related to resiliency in the context of this new model may be reduced to instances of BO-WSP.
Justice familiale et migration : de la diversité culturelle à la double appartenance
Authors: Wyvekens Anne 
Published: December 2016

À partir d’une enquête menée dans deux tribunaux de grande instance, l’article se propose de montrer comment la justice familiale, lorsqu’elle est confrontée à des justiciables immigrés ou d’origine immigrée, met en scène, plus qu’une différence entre « eux » et « nous », une double appartenance, produit complexe de la migration. Annulations de mariage et changements de prénom font voir des justiciables qui sont à la fois d’ici et d’ailleurs. Quant aux magistrats, ils abordent ces situations en naviguant entre un traitement pragmatique – relativement indifférent aux différences – des situations individuelles, et des représentations parfois suspicieuses de l’immigration en tant que phénomène collectif.
Introduction : génétique et théâtre
Authors: Simonet-Tenant Françoise 
Published: December 2016

Après avoir rappelé les étapes qui ont permis au domaine de la « critique génétique » de se formuler, Françoise Simonet-Tenant récapitule ici les caractéristiques spécifiques de la genèse théâtrale. Le texte et la représentation s’organisent en effet souvent ensemble. Dès lors, le nombre d’intervenants dans l’élaboration de la pièce augmente et ne se cantonne plus seulement à « l’auteur », et la chronologie préparatoire de la pièce se révèle plus difficile à tracer. Celle-ci semble être en fait toujours dans un état transitoire, à renouveler sans cesse au gré des représentations successives.
Sex, Lies et les Franco-Manitobains de Marc Prescott : de l’esclandre étudiant (1993) au classique avant-gardiste (2013)
Authors: Ladouceur Louise 
Published: December 2016

Louise Ladouceur analyse ici le cas d’une pièce de théâtre de Marc Prescott, un dramaturge bilingue de l’Ouest canadien, maîtrisant à la fois le français et l’anglais. La pièce étudiée met en scène une parole mêlant ces deux langues, ainsi qu’on peut l’entendre dans le Manitoba contemporain (1993-2013). C’est toute la relation entre les deux langues et les deux communautés dans cette région de l’Ouest du Canada qui est donc mise en question ici. L’étude des différentes révisions du texte entre les représentations de 1993, à Saint-Boniface au Manitoba, et de 2013, au Québec, permet de comprendre tous les enjeux politiques, sociaux et culturels que recouvre cette question d’un « bilinguisme » affiché mais plus ou moins bien assumé. Les différentes révisions du texte en fonction des publics concernés (le Manitoba, le Québec) montrent aussi comment dans différentes régions francophones du Canada se joue le rapport aux deux langues officielles du pays.
« Ces belles voix à la dérive » : Jules Supervielle et le désir de théâtre
Authors: Davaille Florence 
Published: December 2016

Florence Davaille étudie ici certains aspects de la genèse d’une pièce de théâtre de Jules Supervielle, Bolivar, jouée en 1936 à la Comédie-Française et reprise sous la forme d’un opéra en 1950. L’objectif est de montrer ce qui est au cœur des ré-écritures successives : incarner des voix dans des personnages, geste de création fondateur dans l’ontologie développée par Supervielle dès l’écriture de sa poésie. Au-delà du passage d’un genre à un autre, le choix d’écrire du théâtre chez Supervielle se révèle être l’étape ultime d’une « poétique de l’incarnation », ce qui rend cet aspect de son œuvre important, même s’il s’agit d’un théâtre très peu connu aujourd’hui.
Epigenetic and Transcriptional Regulation of IRAK-M Expression in Macrophages
Authors: Lyroni Konstantina , Patsalos Andreas , Daskalaki Maria G. , Doxaki Christina , Soennichsen Birte , Helms Mike , Liapis Ioannis , Zacharioudaki Vassiliki , Kampranis Sotirios C. , Tsatsanis Christos 
Published: December 2016

Abstract
During macrophage activation, expression of IL-1R–associated kinase (IRAK)-M is induced to suppress TLR-mediated responses and is a hallmark of endotoxin tolerance. Endotoxin tolerance requires tight regulation of genes occurring at the transcriptional and epigenetic levels. To identify novel regulators of IRAK-M, we used RAW 264.7 macrophages and performed a targeted RNA interference screen of genes encoding chromatin-modifying enzymes, signaling molecules, and transcription factors involved in macrophage activation. Among these, the transcription factor CCAAT/enhancer binding protein (C/EBP)β, known to be involved in macrophage inactivation, was necessary for the induction of IRAK-M expression. Chromatin immunoprecipitation showed that C/EBPβ was recruited to the IRAK-M promoter following LPS stimulation and was indispensable for IRAK-M transcriptional activation. Among histone 3–modifying enzymes, our screen showed that knockdown of the histone 3 lysine 27 (H3K27) methyltransferase and part of the polycomb recessive complex 2, enhancer of Zeste 2, resulted in IRAK-M overexpression. In contrast, knockdown of the H3K27 demethylase ubiquitously transcribed tetratricopeptide repeat X chromosome suppressed the induction of IRAK-M in response to LPS stimulation. Accordingly, we demonstrated that H3K27 on the IRAK-M promoter is trimethylated in unstimulated cells and that this silencing epigenetic mark is removed upon LPS stimulation. Our data propose a mechanism for IRAK-M transcriptional regulation according to which, in the naive state, polycomb recessive complex 2 repressed the IRAK-M promoter, allowing low levels of expression; following LPS stimulation, the IRAK-M promoter is derepressed, and transcription is induced to allow its expression.