bluuebunny/crossref_metadata_2025_split huggingface dataset:
BINARY VECTOR CONSTRUCTION OF KAPPA(κ)-CARRAGEENASE GENE AND TRANSFORMATION TO Agrobacterium tumefaciens AS MEDIATOR FOR SEAWEED TRANSGENIC GENERATION
Authors: Rajamuddin Muh Alias L. , Alimuddin . , Widyastuti Utut , Harris Enang , Suryati Emma 
Published: January 2018

Increasing of kappa (κ)-carrageenan content in Kappaphycus alvarezii seaweed is potentially be achieved by applying transgenesis technology. This study was performed to obtain a construction of κ-Carrageenase gene and Agrobacterium tumefaciens to carry those construction genes. The κ-Carrageenase (κ-Car) gene was involved in κ-carrageenan biosynthesis. The κ-Car gene sequence was ligated between the 35S CaMV promoter and tNos terminator sequences to generate pMSH/κ-Car expression vector. Transformation of pMSH/κ-Car plasmid to Escherichia coli was performed by heat-shock method, and to Agrobacterium tumefaciens by tri-parental mating method. The results showed that several colonies of E. coli and A. tumefaciens grew in the selective culture mediums containing antibiotic. PCR analysis using primers 35S-Forward and tNos-Reverse with DNA template from those bacterial colonies resulted DNA fragment of about 2,000 bp, the same as the total length of 35S CaMV promoter, κ-Car gene and tNos terminator sequences. Therefore, the construction of pMSH/κ-Car gene was succeeded and a colony of A. tumefaciens transformant carrying pMSH/κ-Car plasmid was successfully produced. Keywords: Agrobacterium tumefaciens, kappa(κ)-Carrageenase gene, transgenesis, vector
BIODIVERSITY AND ABUNDANCE OF DEMERSAL FISHS STOCK IN TAMBELAN WATER, NATUNA SEA
Authors: Fahmi . , Yonvitner . 
Published: January 2018

Understanding on fish diversity is essential for fish stock conservation specifically in Tambelan Island waters with a high fish diversity. This research was conducted in November 2010 at 6 sampling site around Tambelan Island. Samples were taken using trawl gear within sweeping technique. The para-meters of collected data were kind of species, number of fish, total length, and total weight. Data analyses were stock abundance (based on time trawling), diversity, richness, equity, and similarity between site sampling. The total fishes that were caugth were 1.224 individual that consisted of 105 species and 44 family of fishes. Total weight of sample was 55.3 kg, with the average stock abundance 0.27 kg/hour. Fishes catch composition were Apogonidae family 11,4%, Mulliidae family 35.7%, Nemipteridae family 16.3%, and Lutjanidae family 7,7%. Richness value index ranged of 1862 –3.121. Equitability index ranged of 0.329 – 0.576. Similarity index for station 3 and 6 was at 33.89% level; for station 1, 4, and 5 was at 20.31 % level, and for station 4 and 5 was at 45,30% level from the maximum value of 100%.
REPRODUCTIVE BIOLOGY OF POKEA CLAM Batissa violacea var. celebensis, VON MARTENS 1897 AT LASOLO ESTUARY, SOUTHEAST SULAWESI
Authors: Bahtiar . 
Published: January 2018

Pokea clam Batissa violacea var. celebensis von Martens 1897 that inhabits the substrates of some estuaries in Southeast Sulawesi has a tendency to have different reproductive pattern. The aim of this study was to determine the sex ratio, gonadal maturity stage (TKG), gonadal maturity index (IKG) and the size at first gonad maturity of pokea in Lasolo estuary, Southeast Sulawesi. Samples were randomly collected using a traditional fishing gear called tangge. Sex ratio, TKG, IKG, and the size at first gonad maturity were determined using standard methods and formulas and then analyzed using Chi Square, quantitative description and non-linear regression. The results showed that the number of males were higher than the numbers of females, ranging from 71.85-89.84% and 10.16-28.15%, respectively. Gonadal maturity development of male and female occured simultaneously. Early gonadal maturity stage began in January. Spawning occurs twice, beginning with maturity in April and spawning in May and then again maturity in July and spawning in July until October. IKG values of male and female were relatively the same ranging from 2.56-31.92. The males matured earlier than the females at size 1.95 cm and 2.15 cm, respectively. Keywords: female, gonad, male, clam, maturity, spawning
TOTAL HEMOCYTE COUNT AND HEMOLYMPH GLUCOSE CONCENRATION RESPONSE OF SPINY LOBSTER Panulirus homarus ON RATIO OF SHELTER
Authors: Djai Suhaiba , Supriyono Eddy , Nirmala Kukuh , Adiyana Kukuh 
Published: January 2018

This research was conducted to assess the physiological response of the lobster Panulirus homarus for the ratio of the shelters. The method used completely randomized design with two replicates of each treatments with shelter ratio (A) 1 : 5, (B) 3 : 5, (C) 4 : 5, (D) 5 : 5. Weight average for 184 lobsters with the stocking density of 23 lobsters for each treatment was 32.64 ± 0.58 g. The experiment was conducted for 60 days. The lobster was fed with trash fish and acclimatized for 7 days before the experiment. Observations on the physiologycal of every 10 days. The physiological responses that observed were total hemocyte count (THC) and hemolymph glucose concentration. The results showed that 4:5 was the best lobster shelter ratio because it could reduce stress levels. This is indicated by the stable values of THC and hemolymph glucose level during the experiment and supported by the growth of 57.28 ± 0.15 g and survival rate of 91.31 ± 2.60%. Keywords: lobster, Panulirus homarus, ratio, shelter, THC, glucose
CARRYING CAPACITY OF EAST BEACH OF BULUKUMBA REGENCY FOR MARINE TOURISM ACTIVITIES
Authors: Wahyuni Andi Panca , Yonvitner . , Setyobudiandi Isdradjad 
Published: January 2018

The purpose of the study was to assess the carrying capacity of East Beach of Bulukumba Regency for tourism activities. East Beach was obtained three marine tourism activities namely beach tourism of recreation, snorkeling and diving. Coastal tonamelyurism/recreation categories according to the total length area that utilized around 1.696 m can accommodate 68 people/day, snorkeling tourism around 71.605 m² can accommodate 286 people/day and diving tourism around 98.534 m² is able to accommodate 394 people/day. Thus the total tourist that can fit to the overall tourism activity around 748 people/day. Keywords : carrying capacity, marine tourism, east beach, Bulukumba regency
SUITABILITY ANALYSIS OF FLOATING CAGE CULTURE OF GROUPER FISH USING GIS IN RINGGUNG WATERS OF LAMPUNG
Authors: Hastari Indah Febry , Kurnia Rahmat , Kamal M. Mukhlis 
Published: January 2018

The study aims to determine suitability of floating cage (KJA) culture of grouper fish in Ringgung waters of Lampung. Data were analysed using GIS for spatial analysis at five stations. There were 10 water quality parameters measured, such as bathimetry, current velocity, water transparency, temperature, salinity, pH, dissolved oxygen, nitrate, ammonia, and phosphate. Three suitability criteria, i.e very suitable, suitable, and not suitable were used to determine the suitability of floating cage culture of grouper fish. The results show that from 99.168,5 ha of the total area used for floating cage culture of grouper fish, about 85,94% covering 8522,16 ha were classified as very suitable, 5,64% covering 559,69 ha classified as suitable, and 8,42% covering 835 ha classified as not suitable. Keywords : grouper culture, suitability, Ringgung waters, Lampung Province, GIS
MANAGEMENT OF MANGROVE ECOSYSTEM POTENCY IN KUALA LANGSA, ACEH
Authors: Zurba Nabil , Effendi Hefni , Yonvitner . 
Published: January 2018

The potency of mangrove ecosystems can be a provider of economic resources, preserving the ecological environment and providing environmental services. The existence of mangroves in Kuala Langsa is important to study about the economic potential, the potential of carbon sequestration in the form of biomass, coastal tourism potential and social potential of society in support of its development efforts. The result of the research shows that the potential existence of mangrove ecosystem to fishery sector is Rp. 657.563.000 / year, carbon sequestration potential in the form of biomass of 180.365 ton /year, with the economic value of carbon trade of Rp. 2.344.745.000/year, economic potential of coastal tourism reached Rp 22,921,107,253/year. Overall, the estimated total potential of existing mangrove ecosystems in Kuala Langsa is Rp. 29.923.415.253/year. To manage these potentials, a conservation strategy is required, performance improvement of customary institutions and the existence of such management institutions must be continuously strengthened. Keywords: institutional, Kuala Langsa, mangrove, potency, strategic management
SEDIMENT ACCUMULATION PROFILE IN MANGROVE RESTORATION AREA OF LEMBAR BAY-LOMBOK ISLAND
Authors: Paputungan Mohammad Sumiran , Koropitan Alan Frendy , Prartono Tri , Lubis Ali Arman 
Published: January 2018

Mangrove restoration is really needed for restoring its ecosystem functions, so that it could be able to support fisheries activity and to protect coastal by extreme weather. In addition, mangrove is able to accumulate sediment that important in protecting the coastal area from sea level rise. Therefore, the aim of this study is to investigate sediment accumulation rate in mangrove area during post restoration. Sampling location were divided into three different stations based on estimated restoration ages, such as ≥ 15 years old (Station 1), 4 - 10 years old (Station 2) and 2 - 8 years old (Station 3). Sediment cores were carried out by inserting 7.6 cm diameter and 100 cm length of polyvinyl chloride pipes. Sedimentation rate is measured by using Pb-210 radionuclide analysis. The results show that the sediment accumulation rate in the last 20th years from all station ranges from 0.17 to 0.42 g/cm2/year. The highest accumulation rate is found at oldest year old station while the lowest accumulation rate is found at younger year old station of mangrove restoration area. Restoration process is clearly able to recover the mangrove’s role in trapping sediment in coastal region. Keywords: sediment accumulation, mangrove restoration, Lembar Bay- Lombok Island
POTENTIAL FISHING GROUND MAPPING BASED ON GIS HOTSPOT MODEL AND TIME SERIES ANALYSIS: A CASE STUDY ON LIFT NET FISHERIES IN SERIBU ISLAND
Authors: Rivai Andi Alamsyah , Siregar Vincentius P. , Agus Syamsul B. , Yasuma Hiroki 
Published: January 2018

Information on the spatial and temporal of fishing activity can optimize a fisheries management and increase their economical and biological benefit. For effective management and good understanding of fishing activities, information about fishing ground is crucial. In this study, we aimed to analyze the spatio-temporal of lift net fisheries in Kepulauan Seribu by analyzing their fishing season, investigating their hotspot of fishing ground using GIS-based hotspot model, and mapping the potential fishing ground of each target species. We found that anchovy and scad could be caught throughtout the year, while sardine and squid had high fishing season in west monsoon. Hotspot of fishing ground of lift net fisheries in Kepulauan Seribu waters generally was concentrated around Lancang Island and in southern part of Kotok Island. Potential fishing ground for sardines was located in around Lancang Island on west monsoon. Squids were highly distributed around Lancang Island in December to January and around Lancang and Rambut Islands in November. Anchovy and scad had more potential fishing ground in around Kepulauan Seribu waters. Keywords: fishing ground, lift net, hotspot, fishing season
ANALISYS AND MAPPING OF ENVIRONMENTAL SENSITIVITY INDEX IN BANGGAI REGENCY AND BANGGAI ISLANDS REGENCY, CENTRAL SULAWESI
Authors: Putranto Sugeng , Zamani Neviaty P. , Sanusi Harpasis S. , Riani Etty , Fahrudin Achmad 
Published: January 2018

The Environmental Sensitivity Index (ESI) is a description of the biological, socio-economic and socio-cultural values of a particular coastal and marine area used as a priority response to oil spills, shown on a map by applying spatial analysis using geographic information system technology. The purpose of this research was to analyse mangrove ecosystem area in Banggai and Banggai Islands Regency based on its vulnerability value by analyzing mangrove community structure and socio-economic services for local community. The research was conducted in nine sub-districts, among others: South Batui, Batui, East Luwuk, Lamala, Masama, Balantak, Bualemo (Banggai) and Bulagi and Buko (Banggai Islands). The study was conducted from August to November 2016, with field observations, direct interviews with communities and local government and literature review. The results of spatial analysis of ESI in the coastal areas of Banggai and Banggai Islands are obtained from sensitivity values of medium and sensitive. Areas with moderate sensitivity are Batui, East Luwuk, Masama, Lamala, Balantak and Bualemo sub-districts with grades of 16,78 – 24,35. The value of ESI with sensitive category ranges from 38,24 – 57,54 in Bulagi, Buko and South Batui sub-districts. Keywords: mangrove ecosystem, Environmental Sensitivity Index (ESI)
LAND CHANGES AND MANAGEMENT STRATEGIES OF MANGROVE IN SAWO DISTRICT, NORTH NIAS REGENCY, NORTH SUMATERA PROVINCE
Authors: Mendrofa Septinus , Kurnia Rahmat , Pratiwi Niken T.M. 
Published: January 2018

One of the resources that has potential to utilization is a mangrove ecosystem. The aim of this study was to analyze the changes in the area of mangroves, analyze the causes of destruction of mangroves, and formulate strategies for sustainability management of mangroves in Sawo District. The changes in the area of mangroves was identified from imagery and analyzed by ArcGis 10.2.2. The causes of mangrove destruction was analyzed from the data collected from the stakeholders in Sawo District that related to management of mangroves in district level, regency level, and province. The data was analyzed by Analytical Hierarchy Process (AHP) to formulate the strategies for sustainability management of mangroves. The result of landsat imagery showed that, mangrove area has decreased about 17,405 ha within a decade. Mangrove destruction was caused by human activities, such as logging and conversion of mangrove land into agriculture. The main strategy for sustainability management of mangroves was community’s empowerment, then followed by others alternatives. Keywords: AHP, coastal, destruction, ecosystem, landsat
SELECTION OF KURAU FISHING TECHNOLOGY UNITS Eleutheronema tetradactylum WHICH COMPETITIVE AND SUSTAINABLE
Authors: Kholis Muhammad Natsir , Wahju Ronny I , Mustaruddin . 
Published: January 2018

Environmentally friendly fishing technology unit is needed in sustainable fisheries management. The purpose of this study was to determine the fishing technology unit of kurau competitive and sustainable. Data collection was carried out from July to September 2016 in the Coastal Pambang of Bengkalis District of Riau Province, by using the survey method. The analytical data method used is scoring the biological, technical and socioeconomic aspects the fishing technology unit of kurau. Results of research show that combined analysis of biological, technical and socioeconomic aspects have the value of the VA fishing line function (2.48) is higher than the other three fishing gear. Thus, the fishing line is a selected fishing technology unit of kurau competitive and sustainable in the Coastal Pambang Bengkalis District. Keywords: competitive,coastal pambang, fishing technology, kurau fish,sustainable fishing
IMPACT OF NURSERY USING DIFFERENT PHYSICAL FILTRATION SYSTEM ON HEMOLYMPH GLUCOSE LEVEL AND SURVIVAL RATE OF SPINY LOBSTER JUVENILE Panulirus homarus
Authors: Prama Ega Aditya , Supriyono Eddy , Nirmala Kukuh , Adiyana Kukuh 
Published: January 2018

The problems in culture of early juvenile phase of spiny lobster Panulirus homarus is low survival rate of the seed. One of strategies to improve production is using filtration system during nursery of spiny lobster. The aim of this study to evaluate differentation of physical filtration system on responsse stress and survival rate of juvenile P. homarus. Experimental design of this study consisted of four treatments and two replications. The treatments were using flow through system without protein skimmer and filter (K), filtration system with protein skimmer (SK), filtration system with top filter (F) and filtration system with combinations of protein skimmer and top filter (SKF). P. homarus with initial weight 0.18+0.01 g were cultured for 60 days with density 250 lobster/tank. During maintenance, P. homarus were fed trash fish with feeding rate 20% of body weight. Parameters of glucose hemolymph were evaluated to determine stress response. Stress response was determined at day 0, 6, 20 and 60. Parameters of survival rate were determined at the end of experiment. The result showed that the treatment of filtration system with combination of protein skimmer and top filter (SKF) decreased stress responsse by decreasing glucose hemolymph of juvenile P. homarus during maintenance. Survival rate also higher (P<0.05) in treatment SKF (33.2%) than other treatments. It is concluded that SKF was the best physical filtration system for juvenile period of spiny lobster P. homarus Keywords: filtration system, glucose hemolymph, spiny lobster, survival rate
CHARACTERISTICS OF BREAKING WAVES AND ANALYSIS OF SEDIMENT TRANSPORT IN TELUK KENDARI
Authors: Ondara Koko , Husrin Semeidi 
Published: January 2018

One of the problems that often occured in the bay area is a sedimentation process that occurred continuously, causing silting. The movement of sediment material in the process of sedimentation is influenced by the movement of the water flow like the tides, winds, currents and waves. The aim of this study was to determine the characteristics of the hydrodynamic conditions, particularly the distribution pattern and the capacity of the sediments in the Kendari Bay with oceanographic modeling approach. Bathymetry data measured at the site of the research conducted in August 2015. The analysis of distribution patterns of sedimentation, sedimentation capacity, wave hindcasting, wind data and tidal data using software, hindcasting results indicate the dominant wind direction coming from the east. Type tides in Kendari Bay is a mixed mainly semiurdunal tides. The concentration of suspended sediment at low tide is greater than at high tide. Keywords: sediment transport, wave characteristics, Kendari Bay, hydro - dynamics, mike21
DISTRIBUTION AND BIOACCUMULATION OF HEAVY METALS Pb AND Cd ON BIVALVES Anadara nodifera, Meretrix lyrata, AND Solen lamarckii IN COASTAL WATERS OF THE WEST MADURA STRAIT
Authors: Ahyar . , Bengen Dietriech G. , Wardiatno Yusli 
Published: January 2018

Madura strait receives waste disposal from various activites in the mainland, making it vulnerable to contamination of harmful pollutants. Some of the harmful pollutants are heavy metal Pb and Cd. The high concentration Cd and Pb give negative impacts to the biota including bivalves. This study was conducted to assess the distribution of heavy metals Pb and Cd in the waters and their accumulation in bivalves Anadara nodifera, Meretrix lyrata, and Solen lamarckii in coastal waters of the West Madura Strait. Heavy metal analysis refer to APHA 2012 using AAS. The results showed that concentration of Cd and Pb in water were below the quality standards, while in sediment were above the standard (Cd of 7.20 mg/kg and Pb of 62.06 mg/kg). The concentration of Cd in bivalve were different among the species tested A. nodifera of 6.10 mg/kg, M. lyrata of 3.65 mg/kg, and S. lamarckii of 2.74 mg/kg, as well as Pb concentration A. nodifera of 60.10 mg/kg, M. lyrata of 51.48 mg/kg, and S. lamarckii of 45.29 mg/kg). These results indicated that the three shells have exceeded the maximum limit of heavy metal contamination in food (Cd of 1.0 mg/kg and Pb of 1.5 mg/kg). Keywords: bivalve, heavy metal, Madura Strait
DIVERSITY OF BRITTLE STARS (OPHIUROIDEA) IN TALISE WATERS, NORTH SULAWESI
Authors: Nurdiansah Doni , Supono . 
Published: January 2018

Talise waters, located in North Sulawesi, is one of many coastal areas in Indonesia with sea grass cover extending along the reef flat. This ecological feature is beneficial for living habitat of associated fauna, including brittle stars. Brittle stars are group of Echinodermata reported to be abundantly found in sea grass ecosystem. However, the diversity of this fauna is less reported except as part of echinoderm studies. This research aims to investigate the diversity of brittle stars in Talise waters. Data were collected in July 2009 applying quadrant transect method. In total of 650 individuals belonging to 4 families (Ophiotrichidae, Ophiodermatidae, Ophiocomidae and Ophiuridae) and 10 species were recorded during this study. The diversity (H’=1.58), richness (D=1.69) and Evennes Index (J=0.89) was relatively higher compared to adjacent water in North Sulawesi such as Wori (H =1.64, D =1.38, J=0.85), Kema (H =1.3, D =0.38, J=0.36) but lower than East Likupang waters (H =1.95, D =2.42, J=0.97). Keywords: brittle star, diversity, richness, evennes, Talise waters
GENETIC DIVERSITY OF OLIVE RIDLEY Lepidochelys olivacea ASSOCIATED WITH CURRENT PATTERN IN CENDRAWASIH BAY, PAPUA
Authors: Bahri Samsul , Atmadipoera Agus S. , Madduppa Hawis H. 
Published: January 2018

Cendrawasih Bay is habitat for olive ridley (Lepidochelys olivacea) which is directly adjacent to the Pacific Ocean. The presence and the diversity of L. olivacea in Cendrawasih Bay has been threatened. Genetics can be the key to conservation because they play an important role in maintaining population and recovering from damage. This study aims to determine the genetic diversity of L. olivacea and its association to the current pattern in the waters of Cendrawasih Bay. Samples of L. olivacea (n=20) were collected in Kwatisore (n=8) and Yapen Island (n=12) in Cendrawasih Bay waters from August 2015 to December 2016. The molecular analysis was based on 791-bp fragment of D-Loop on the non-coding region gen. The current pattern analysis was performed through INDESO data and visualized by using Ferret software. Phylogenetic analysis showed that 2 groups of L. olivacea from a total of 2 haplotypes, whose population from Kwatisore was dispersed in two haplotypes, while the population from Yapen Island was only dispersed in one haplotype. Populations from Kwatisore showed higher variations than populations from Yapen Island. The current pattern analysis suggests that the two study sites, Kwatisore and Yapen islands are different. Both populations are only connected by the Northwest monsoon currents period that indicates a little geneflow between this populations. Thus causing differences variation between Kwatisore and Yapen Island populations genetically. Keywords: genetic diversity, current pattern, coral triangle, olive ridley
Thermodynamic description of the Cu–S–Sn system
Authors: Guo Cuiping , Zhang Xiaobo , Li Changrong , Du Zhenmin 
Published: January 2018

AbstractThe thermodynamic optimisation of the Cu–S–Sn system was carried out using the CALPHAD technique. For the liquid phase, an associated solution model, (Cu, Cu2S, S, SnS, Sn), was used by assuming the existence of two associates: Cu2S and SnS. Ternary compounds Cu5Sn2S7and Cu10Sn2S13were treated as stoichiometric compounds. Ternary compounds Cu4SnS4, Cu2SnS3, and Cu4Sn3S8were located on the Cu2S–SnS2joint and described using the model (Cu2S)p(SnS2)q. The Cu2Sn3S7compound was also located on the Cu2S–SnS2joint and was described as (Cu2S, SnS2) considering the range of its homogeneity. With the present thermodynamic parameters, the projection of the liquidus surfaces and invariant reactions of the Cu–S–Sn system were predicted. A set of self-consistent thermodynamic parameters of the Cu–S–Sn system could be obtained.