bluuebunny/crossref_metadata_2025_split huggingface dataset:
Germline Variants in DNA Repair Genes, Diagnostic Radiation, and Risk of Thyroid Cancer
Authors: Sandler Jason E. , Huang Huang , Zhao Nan , Wu Weiwei , Liu Fangfang , Ma Shuangge , Udelsman Robert , Zhang Yawei 
Published: December 2017

Abstract
Background: Radiation exposure is a well-documented risk factor for thyroid cancer; diagnostic imaging represents an increasing source of exposure. Germline variations in DNA repair genes could increase risk of developing thyroid cancer following diagnostic radiation exposure. No studies have directly tested for interaction between germline mutations and radiation exposure.
Methods: Using data and DNA samples from a Connecticut population–based case–control study performed in 2010 to 2011, we genotyped 440 cases of incident thyroid cancer and 465 population-based controls for 296 SNPs in 52 DNA repair genes. We used multivariate unconditional logistic regression models to estimate associations between each SNP and thyroid cancer risk, as well as to directly estimate the genotype–environment interaction between each SNP and ionizing radiation.
Results: Three SNPs were associated with increased risk of thyroid cancer and with thyroid microcarcinoma: HUS rs2708896, HUS rs10951937, and MGMT rs12769288. No SNPs were associated with increased risk of larger tumor (&gt;10 mm) in the additive model. The gene–environment interaction analysis yielded 24 SNPs with Pinteraction &lt; 0.05 for all thyroid cancer, 12 SNPs with Pinteraction &lt; 0.05 for thyroid microcarcinoma, and 5 SNPs with Pinteraction &lt; 0.05 for larger tumors.
Conclusions: Germline variants in DNA repair genes are associated with thyroid cancer risk and are differentially associated with thyroid microcarcinoma and large tumor size. Our study provides the first evidence that germline genetic variations modify the association between diagnostic radiation and thyroid cancer risk.
Impact: Thyroid microcarcinoma may represent a distinct subset of thyroid cancer. The effect of diagnostic radiation on thyroid cancer risk varies by germline polymorphism. Cancer Epidemiol Biomarkers Prev; 27(3); 285–94. ©2017 AACR.
Helicobacter pylori Blood Biomarkers and Gastric Cancer Survival in China
Authors: Varga Matthew G. , Wang Tianyi , Cai Hui , Xiang Yong-Bing , Gao Yu-Tang , Ji Bu-Tian , Pawlita Michael , Waterboer Tim , Zheng Wei , Shu Xiao-Ou , Epplein Meira 
Published: December 2017

Abstract
Background: Infection with Helicobacter pylori is the leading risk factor for noncardia gastric cancer, yet its influence on prognosis of gastric cancer is largely unknown. Thus, exploring the role of Helicobacter pylori (H. pylori) in survival could lead to a greater understanding of the high mortality associated with gastric cancer.
Methods: Seropositivity to 15 H. pylori antigens was assessed using a multiplex assay in two prospective cohorts, the Shanghai Men's Health Study and the Shanghai Women's Health Study. Multivariable-adjusted Cox proportional hazards regression was used to examine the association between prediagnostic H. pylori antigen levels and gastric cancer–specific survival.
Results: Prediagnostic levels of H. pylori serum antibodies that were previously associated with gastric cancer incidence in this population were not associated with gastric cancer survival, whether assessed in a 6-antigen panel [HR = 1.29; 95% confidence interval (CI), 0.78–2.13 for men; HR = 0.93; 95% CI, 0.57–1.52 for women], focused on CagA+H. pylori (HR = 0.73; 95% CI, 0.44–1.20 forwomen; HR = 1.27; 95% CI, 0.70–2.31 for men) or on the high-risk biomarkers of dual Omp and HP 0305 seropositivity (HR = 0.97; 95% CI, 0.72–1.30 for women; HR = 1.37; 95% CI, 0.97–1.94 for men).
Conclusions: Prediagnostic H. pylori antigen levels are not associated with gastric cancer survival in East Asian populations.
Impact: Identification of additional factors associated with gastric cancer survival would further our understanding of the high mortality associated with this malignancy. Cancer Epidemiol Biomarkers Prev; 27(3); 342–4. ©2017 AACR.
Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer
Authors: Tucker Douglass W. , Getchell Christopher R. , McCarthy Eric T. , Ohman Anders W. , Sasamoto Naoko , Xu Shuyun , Ko Joo Yeon , Gupta Mamta , Shafrir Amy , Medina Jamie E. , Lee Jonathan J. , MacDonald Lauren A. , Malik Ammara , Hasselblatt Kathleen T , Li Wenjing , Zhang Hong , Kaplan Samuel J. , Murphy George F. , Hirsch Michelle S. , Liu Joyce F. , Matulonis Ursula A. , Terry Kathryn L. , Lian Christine G. , Dinulescu Daniela M. 
Published: December 2017

Abstract
Purpose: A major challenge in platinum-based cancer therapy is the clinical management of chemoresistant tumors, which have a largely unknown pathogenesis at the level of epigenetic regulation.
Experimental Design: We evaluated the potential of using global loss of 5-hydroxymethylcytosine (5-hmC) levels as a novel diagnostic and prognostic epigenetic marker to better assess platinum-based chemotherapy response and clinical outcome in high-grade serous tumors (HGSOC), the most common and deadliest subtype of ovarian cancer. Furthermore, we identified a targetable pathway to reverse these epigenetic changes, both genetically and pharmacologically.
Results: This study shows that decreased 5-hmC levels are an epigenetic hallmark for malignancy and tumor progression in HGSOC. In addition, global 5-hmC loss is associated with a decreased response to platinum-based chemotherapy, shorter time to relapse, and poor overall survival in patients newly diagnosed with HGSOC. Interestingly, the rescue of 5-hmC loss restores sensitivity to platinum chemotherapy in vitro and in vivo, decreases the percentage of tumor cells with cancer stem cell markers, and increases overall survival in an aggressive animal model of platinum-resistant disease.
Conclusions: Consequently, a global analysis of patient 5-hmC levels should be included in future clinical trials, which use pretreatment with epigenetic adjuvants to elevate 5-hmC levels and improve the efficacy of current chemotherapies. Identifying prognostic epigenetic markers and altering chemotherapeutic regimens to incorporate DNMTi pretreatment in tumors with low 5-hmC levels could have important clinical implications for newly diagnosed HGSOC disease. Clin Cancer Res; 24(6); 1389–401. ©2017 AACR.
A Transgenic Dual-Luciferase Reporter Mouse for Longitudinal and Functional Monitoring of T Cells In Vivo
Authors: Szyska Martin , Herda Stefanie , Althoff Stefanie , Heimann Andreas , Russ Josefine , D'Abundo Daniele , Dang Tra My , Durieux Isabell , Dörken Bernd , Blankenstein Thomas , Na Il-Kang 
Published: December 2017

Abstract
Adoptive T-cell therapy (ATT) efficacy is limited when targeting large solid tumors. The evaluation of ATT outcomes using accessory treatment would greatly benefit from an in vivo monitoring tool, allowing the detection of functional parameters of transferred T cells. Here, we generated transgenic bioluminescence imaging of T cells (BLITC) mice expressing an NFAT-dependent click-beetle luciferase and a constitutive Renilla luciferase, which supports concomitant in vivo analysis of migration and activation of T cells. Rapid transferability of our system to preestablished tumor models was demonstrated in the SV40-large T antigen model via both crossbreeding of BLITC mice into a T-cell receptor (TCR)-transgenic background and TCR transduction of BLITC T cells. We observed rapid tumor infiltration of BLITC CD8+ T cells followed by a burst-like activation that mirrored rejection kinetics. Using the BLITC reporter in the clinically relevant H-Y model, we performed female to male transfers and detected H-Y-specific alloreactivity (graft-versus-host disease) in vivo. In an H-Y solid tumor model, we found migration of adoptively transferred H-Y TCR-transgenic CD4+ T cells into the tumor, marked by transient activation. This suggests a rapid inactivation of infiltrating T cells by the tumor microenvironment, as confirmed by their expression of inhibitory receptors. In summary, the BLITC reporter system facilitates analysis of therapeutic parameters for ATT, is rapidly transferable to models of interest not restricted to tumor research, and is suitable for rapid screening of TCR clones for tumor rejection kinetics, as well as off-target effects. Cancer Immunol Res; 6(1); 110–20. ©2018 AACR.
Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury
Authors: Chimenti Laura , Camprubí-Rimblas Marta , Guillamat-Prats Raquel , Gomez Maria , Tijero Jessica , Blanch Lluis , Artigas Antonio 
Published: December 2017

Objective Alveolar macrophages play a key role in the development and resolution of acute respiratory distress syndrome (ARDS), modulating the inflammatory response and the coagulation cascade in lungs. Anti-coagulants may be helpful in the treatment of ARDS. This study investigated the effects of nebulized heparin on the role of alveolar macrophages in limiting lung coagulation and inflammatory response in an animal model of acute lung injury (ALI).
Methods Rats were randomized to four experimental groups. In three groups, ALI was induced by intratracheal instillation of lipopolysaccharide (LPS) and heparin was nebulized at constant oxygen flow: the LPS/Hep group received nebulized heparin 4 and 8 hours after injury; the Hep/LPS/Hep group received nebulized heparin 30 minutes before and 4 and 8 hours after LPS-induced injury; the LPS/Sal group received nebulized saline 4 and 8 hours after injury. The control group received only saline. Animals were exsanguinated 24 hours after LPS instillation. Lung tissue, bronchoalveolar lavage fluid (BALF) and alveolar macrophages isolated from BALF were analysed.
Results LPS increased protein concentration, oedema and neutrophils in BALF as well as procoagulant and proinflammatory mediators in lung tissue and alveolar macrophages. In lung tissue, nebulized heparin attenuated ALI through decreasing procoagulant (tissue factor, thrombin–anti-thrombin complexes, fibrin degradation products) and proinflammatory (interleukin 6, tumour necrosis factor alpha) pathways. In alveolar macrophages, nebulized heparin reduced expression of procoagulant genes and the effectors of transforming growth factor beta (Smad 2, Smad 3) and nuclear factor kappa B (p-selectin, CCL-2). Pre-treatment resulted in more pronounced attenuation.
Conclusion Nebulized heparin reduced pulmonary coagulopathy and inflammation without producing systemic bleeding, partly by modulating alveolar macrophages.
Clinical Decision Rules for Pulmonary Embolism in Hospitalized Patients: A Systematic Literature Review and Meta-analysis
Authors: Bass Anne , Fields Kara , Goto Rie , Turissini Gregory , Dey Shirin , Russell Linda 
Published: December 2017

Background Clinical decision rules (CDRs) for pulmonary embolism (PE) have been validated in outpatients, but their performance in hospitalized patients is not well characterized.
Objectives The goal of this systematic literature review was to assess the performance of CDRs for PE in hospitalized patients.
Methods We performed a structured literature search using Medline, EMBASE and the Cochrane library for articles published on or before January 18, 2017. Two authors reviewed all titles, abstracts and full texts. We selected prospective studies of symptomatic hospitalized patients in which a CDR was used to estimate the likelihood of PE. The diagnosis of PE had to be confirmed using an accepted reference standard. Data on hospitalized patients were solicited from authors of studies in mixed populations of outpatients and hospitalized patients. Study characteristics, PE prevalence and CDR performance were extracted. The methodological quality of the studies was assessed using the QUADAS instrument.
Results Twelve studies encompassing 3,942 hospitalized patients were included. Studies varied in methodology (randomized controlled trials and observational studies) and reference standards used. The pooled sensitivity of the modified Wells rule (cut-off ≤ 4) in hospitalized patients was 72.1% (95% confidence interval [CI], 63.7–79.2) and the pooled specificity was 62.2% (95% CI, 52.6–70.9). The modified Wells rule (cut-off ≤ 4) plus D-dimer testing had a pooled sensitivity 99.7% (95% CI, 96.7–100) and pooled specificity 10.8% (95% CI, 6.7–16.9). The efficiency (proportion of patients stratified into the ‘PE unlikely’ group) was 8.4% (95% CI, 4.1–16.5), and the failure rate (proportion of low likelihood patients who were diagnosed with PE during follow-up) was 0.1% (95% CI, 0–5.3).
Conclusion In symptomatic hospitalized patients, use of the Wells rule plus D-dimer to rule out PE is safe, but allows very few patients to forgo imaging.
Actividad reproductiva y dieta de Hemidactylus frenatus (Sauria: Gekkonidae) en el norte de Colombia
Authors: Díaz-Pérez Jorge A. , Sampedro-Marín Alcides C. , Ramírez-Pinilla Martha P. 
Published: December 2017

Despite its success as an invasive species, little is known about the ecological aspects of the gekkonid lizard Hemidactylus frenatus in Colombia. In the present study the size at maturity, sexual dimorphism, reproductive activity, and diet composition of a population of this species in an urban locality of Northern Colombia were determined. We conducted eleven samplings from September 2011 to August 2012 in buildings of the municipality of Sincelejo. A total of 264 specimens H. frenatus were captured, 112 were adult females, 133 adult males and 19 juveniles. Males reach sexual maturity at a smaller size (snout-vent length) than females (males: 35.7 mm; females: 42.7 mm), also they are larger and have proportionally larger heads and mouths than females. Males were reproductive throughout the year; although testicular volume varied significantly between samples, this variation was not associated with body size and precipitation in the study area. Reproductive adult females were found during all the sampling period. Females have an invariable clutch size of two eggs and we found no differences in the diameter and weight of eggs in each oviduct. The diet of H. frenatus is varied, with Diptera, Hemiptera and Formicidae being the prey types with the greatest relative importance values. Individuals of both sexes consume a similar volume and number of prey. Thus, the studied population of H. frenatus has continuous reproductive activity and a generalist-opportunistic feeding behavior. The climatic conditions of the study area, environmental availability of prey and intrinsic features of this species appear to be responsible for their abundance and colonizing success in this and other localities.
First record of Gryllophila skrjabini Sergiev, 1923 and Cephalobellus magalhaesi Schwenk, 1926 (Nematoda: Thelastomatidae) parasites of Neocurtilla claraziana Saussure, 1874 (Orthoptera: Gryllotalpidae) in Argentina
Authors: Rusconi José Matias , Achinelly Maria Fernanda , Camino Nora Beatriz 
Published: December 2017

Thelastomatidae is one of the largest families parasitizing insects, within the order Oxyurida. In this work we reported parasitism in nymphs and adults of Neocurtilla claraziana by two different thelastomatid species as a part of a field survey on agricultural pests. Nymphs and adults of this insect were isolated from grasslands of Buenos Aires State, Argentina using a tensio-active solution. The nematode species Gryllophila skrjabini Sergiev, 1923 and Cephalobellus magalhaesi Schwenk, 1926 are briefly described and measurements are given. Both nematodes are reported for the first time in Argentina with C. magalhaesi being the second isolation of this species in the world. Neocurtilla clarziana is a new host record for G. skrjabini.
Mobile X-Pol Radar: A New Tool for Investigating Pyroconvection and Associated Wildfire Meteorology
Authors: McCarthy Nicholas , McGowan Hamish , Guyot Adrien , Dowdy Andrew 
Published: December 2017

AbstractThe process of pyroconvection occurs when fire-released heat, moisture, and/or aerosols induce or augment convection in the atmosphere. Prediction of pyroconvection presents a set of complex problems for meteorologists and wildfire managers. In particular, the turbulent characteristics of a pyroconvective plume exert bidirectional feedback on fire behavior, often with resulting severe impacts on life and property. Here, we present the motivation, field strategy, and initial results from the Bushfire Convective Plume Experiment, which through the use of mobile radar aims to quantify the kinematics of pyroconvection and its role in fire behavior. The case studies presented include world-first observations from two wildfires and one prescribed burn using the University of Queensland’s portable, dual-polarized X-band Doppler radar (UQ-XPOL). The initial analyses of reflectivity, Doppler winds, polarimetric variables, and spectrum width data provide insights into these relatively unexplored datasets within the context of pyroconvection. Weather radar data are supported by mesonet observations, time-lapse photography, airborne multispectral imaging, and spot-fire mapping. The ability to combine ground-validated fire intensity and progression at an hourly scale with quantitative data documenting the evolution of the convective plume kinematics at the scale of hundreds of meters represents a new capability for advancing our understanding of wildfires. The results demonstrate the suitability of portable, dual-polarized X-band Doppler radar to investigate pyroconvection and associated plume dynamics.
Detecting Severe Weather Trends Using an Additive Regressive Convective Hazard Model (AR-CHaMo)
Authors: Rädler Anja T. , Groenemeijer Pieter , Faust Eberhard , Sausen Robert 
Published: December 2017

AbstractA statistical model for the occurrence of convective hazards was developed and applied to reanalysis data to detect multidecadal trends in hazard frequency. The modeling framework is based on an additive logistic regression for observed hazards that exploits predictors derived from numerical model data. The regression predicts the probability of a severe hazard, which is considered as a product of two components: the probability that a storm occurs and the probability of the severe hazard, given the presence of a storm [P(severe) = P(storm) × P(severe|storm)]. The model was developed using lightning data as an indication of thunderstorm occurrence and hazard reports across central Europe. Although it uses only two predictors per component, it is capable of reproducing the observed spatial distribution of lightning and yields realistic annual cycles of lightning, hail, and wind fairly accurately. The model was applied to ERA-Interim (1979–2016) across Europe to detect any changes in lightning, hail, and wind hazard occurrence. The frequency of conditions favoring lightning, wind, and large hail has increased across large parts of Europe, with the exception of the southwest. The resulting predicted occurrence of 6-hourly periods with lightning, wind, and large hail has increased by 16%, 29%, and 41%, respectively, across western and central Europe and by 23%, 56%, and 86% across Germany and the Alps during the period considered. It is shown that these changes are caused by increased instability in the reanalysis rather than by changes in midtropospheric moisture or wind shear.
High-Resolution Temperature Datasets in Portugal from a Geostatistical Approach: Variability and Extremes
Authors: Fonseca A. R. , Santos J. A. 
Published: December 2017

AbstractClimate research in Portugal is often constrained by the lack of homogeneous, temporally and spatially consistent, and long-term climatic series. To overcome this limitation, the authors developed new high-resolution gridded datasets (~1 km) of daily mean, minimum, and maximum air temperatures over Portugal (1950–2015, 66 yr), based on gridded daily temperatures (E-OBS) at ~25-km spatial resolution. A two-step approach was followed, under the assumption that daily temperature variability in Portugal is mainly controlled by atmospheric large-scale forcing, while local processes are mostly expressed as strong spatial gradients. First, monthly baseline (1971–2000) patterns were estimated at 1-km grid resolution by applying multivariate linear regressions (exploratory variables: elevation, latitude, and distance to coastline). A kriging of residuals from baseline normals of 36 weather stations was applied for bias corrections. Second, bilinearly interpolated daily temperature anomalies were then added to the daily baseline patterns to obtain the final datasets. The method performance was evaluated using fivefold cross-validations. The datasets were also validated using daily temperatures from 23 stations not incorporated in E-OBS. A climatological analysis based on these datasets was carried out, highlighting spatial heterogeneities, seasonality, long-term trends, interannual variability, and extremes. The spatial and temporal variability is generally coherent with previous studies at coarser resolutions. An overall warming trend is apparent for all variables and indices, but showing different strengths and spatial variability. These datasets show important advantages over preexisting data, including more detailed and accurate information on trends and interannual variability of precipitation extremes, and can thus be applied to several areas of research in Portugal, such as hydrology, ecology, agriculture, and forestry.
Unraveling Causes for the Changing Behavior of the Tropical Indian Ocean in the Past Few Decades
Authors: Zhang Lei , Han Weiqing , Sienz Frank 
Published: December 2017

AbstractObservations show that decadal (10–20 yr) to interdecadal (>20 yr) variability of the tropical Indian Ocean (TIO) sea surface temperature (SST) closely follows that of the Pacific until the 1960s. Since then, the TIO SST exhibits a persistent warming trend, whereas the Pacific SST shows large-amplitude fluctuations associated with the interdecadal Pacific oscillation (IPO), and the decadal variability of the TIO SST is out of phase with that of the Pacific after around 1980. Here causes for the changing behavior of the TIO SST are explored, by analyzing multiple observational datasets and the recently available large-ensemble simulations from two climate models. It is found that on interdecadal time scales, the persistent TIO warming trend is caused by emergence of anthropogenic warming overcoming internal variability, while the time of emergence occurs much later in the Pacific. On decadal time scales, two major tropical volcanic eruptions occurred in the 1980s and 1990s causing decadal SST cooling over the TIO during which the IPO was in warm phase, yielding the out-of-phase relation. The more evident fingerprints of external forcing in the TIO compared to the Pacific result from the much weaker TIO internal decadal–interdecadal variability, making the TIO prone to the external forcing. These results imply that the ongoing warming and natural external forcing may make the Indian Ocean more active, playing an increasingly important role in affecting regional and global climate.