bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endogenous peripheral hydrogen sulfide is propyretic: its permissive role in brown adipose tissue thermogenesis in rats
Authors: Soriano Renato N. , Braga Sara P. , Breder Jéssica S. C. , Batalhao Marcelo E. , Oliveira‐Pelegrin Gabriela R. , Ferreira Luiz Fernando R. , Rocha Maria José A. , Carnio Evelin C. , Branco Luiz G. S. 
Published: December 2017

New Findings
What is the central question of this study?
In fever, the most striking response in the acute phase
reaction of systemic inflammation, plasma H2S concentration increases. However, the role of endogenous peripheral H2S in fever is unknown.

What is the main finding and its importance?
Endogenous peripheral H2S is permissive for increased brown adipose tissue thermogenesis to maintain thermal homeostasis in cold environments as well as to mount fever. This finding expands the physiological role of the gaseous modulator as a key regulator of thermal control in health (thermal homeostasis) and disease (fever in systemic inflammation).

AbstractIn recent years, hydrogen sulfide (H2S) has been reported as a gaseous modulator acting in several tissues in health and disease. In animal models of systemic inflammation, the plasma H2S concentration increases in response to endotoxin (bacterial lipopolysaccharide, LPS). The most striking response in the acute phase reaction of systemic inflammation is fever, but we found no reports of the peripheral action of H2S on this thermoregulatory response. We aimed at investigating whether endogenous systemic H2S modulates LPS‐induced fever. A temperature datalogger capsule was inserted in the abdominal cavity of male Wistar rats (220–270 g) to record body core temperature. These animals received an i.p. injection of a systemic H2S inhibitor (propargylglycine; 50 or 75 mg kg−1), immediately followed by an i.p. injection of LPS (50 or 2500 μg kg−1), and were exposed to different ambient temperatures (16, 22 or 27°C). At 22°C, but not at 27°C, propargylglycine at 75 mg kg−1 significantly attenuated (P < 0.0001) the fever induced by LPS (50 μg kg−1), indicating a modulatory (permissive) action of endogenous peripheral H2S on brown adipose tissue (BAT) thermogenesis. Evidence on the modulatory role of peripheral H2S in BAT thermogenesis was strengthened when we discarded (i) the possible influence of the gas on febrigenic signalling (when measuring plasma cytokines), and (ii) its interaction with the nitric oxide pathway, and mainly when (iii) we carried out physiological and pharmacological activations of BAT. Endogenous peripheral H2S modulates (permits) BAT activity not only in fever but also during maintenance of thermal homeostasis in cold environments.
Update from a 12-Year Nationwide Fungemia Surveillance: Increasing Intrinsic and Acquired Resistance Causes Concern
Authors: Astvad K. M. T. , Johansen H. K. , Røder B. L. , Rosenvinge F. S. , Knudsen J. D. , Lemming L. , Schønheyder H. C. , Hare R. K. , Kristensen L. , Nielsen L. , Gertsen J. B. , Dzajic E. , Pedersen M. , Østergård C. , Olesen B. , Søndergaard T. S. , Arendrup M. C. 
Published: December 2017

ABSTRACTNew data from the years 2012 to 2015 from the Danish National Fungemia Surveillance are reported, and epidemiological trends are investigated in a 12-year perspective (2004 to 2015). During 2012 to 2015, 1,900 of 1,939 (98%) fungal bloodstream isolates were included. The average incidence was 8.4/100,000 inhabitants, and this appears to represent a stabilizing trend after the increase to 10.1/100,000 in 2011. The incidence was higher in males than females (10.0 versus 6.8) and in patients above 50 years, and those changes were mainly driven by an increasing incidence among 80-to-89-year-old males (65.3/100,000 in 2014 to 2015). The proportion ofCandida albicansisolates decreased from 2004 to 2015 (64.4% to 42.4%) in parallel with a doubling of the proportion ofCandida glabrataisolates (16.5% to 34.6%,P< 0.0001).C. glabratawas more common among females (34.0% versus 30.4% in males). Following an increase in 2004 to 2011, the annual drug use stabilized during the last 2 to 3 years of that time period but remained higher than in other Nordic countries. This was particularly true for the fluconazole and itraconazole use in the primary health care sector, which exceeded the combined national levels of use of these compounds in each of the other Nordic countries. Fluconazole susceptibility decreased (68.5%, 65.2%, and 60.6% in 2004 to 2007, 2008 to 2011, and 2012 to 2015, respectively,P< 0.0001), and echinocandin resistance emerged inCandida(0%, 0.6%, and 1.7%, respectively,P< 0.001). Amphotericin B susceptibility remained high (98.7%). Among 16 (2.7%) echinocandin-resistantC. glabrataisolates (2012 to 2015), 13 harbored FKS mutations and 5 (31%) were multidrug resistant. The epidemiological changes and the increased incidence of intrinsic and acquired resistance emphasize the importance of continued surveillance and of strengthened focus on antifungal stewardship.
Subcellular Localization and Functional Characterization of GII.4 Norovirus-Encoded NTPase
Authors: Yen Ju-Bei , Wei Ling-Huei , Chen Lee-Wen , Chen Li-Yu , Hung Chien-Hui , Wang Shie-Shan , Chang Pey-Jium 
Published: December 2017

ABSTRACT
The genotype II.4 (GII.4) variants of human noroviruses (HuNVs) are recognized as the major agent of global gastroenteritis outbreaks. Due to the lack of an efficient cell culture system for HuNV propagation, the exact roles of HuNV-encoded nonstructural proteins (including Nterm, NTPase, P22, VPg, Pro, and RdRp) in viral replication or pathogenesis have not yet been fully understood. Here, we report the molecular characterization of the GII.4 HuNV-encoded NTPase (designated GII-NTPase). Results from our studies showed that GII-NTPase forms vesicular or nonvesicular textures in the cell cytoplasm, and the nonvesicular fraction of GII-NTPase significantly localizes to the endoplasmic reticulum (ER) or mitochondria. Deletion analysis revealed that the N-terminal 179-amino-acid (aa) region of GII-NTPase is required for vesicle formation and for ER colocalization, whereas the C-terminal region is involved in mitochondrial colocalization. In particular, two mitochondrion-targeting domains were identified in the C-terminal region of GII-NTPase which perfectly colocalized with mitochondria when the N-terminal region of GII-NTPase was deleted. However, the corresponding C-terminal portions of NTPase derived from the GI HuNV did not show mitochondrial colocalization. We also found that GII-NTPase physically interacts with itself as well as with Nterm and P22, but not VPg, Pro, and RdRp, in cells. The Nterm- and P22-interacting region was mapped to the N-terminal 179-aa region of GII-NTPase, whereas the self-assembly of GII-NTPase could be achieved via a head-to-head, tail-to-tail, or head-to-tail configuration. More importantly, we demonstrate that GII-NTPase possesses a proapoptotic activity, which can be further enhanced by coexpression with Nterm or P22.

IMPORTANCE
Despite the importance of human norovirus GII.4 variants in global gastroenteritis outbreaks, the basic biological functions of the viral nonstructural proteins in cells remain rarely investigated. In this report, we focus our studies on characteristics of the GII.4 norovirus-encoded NTPase (GII-NTPase). We unexpectedly find that GII-NTPase can perfectly colocalize with mitochondria after its N-terminal region is deleted. However, such a phenomenon is not observed for NTPase encoded by a GI strain. We further reveal that the N-terminal 179-aa region of GII-NTPase is sufficient to mediate (i) vesicle formation, (ii) ER colocalization, (iii) the interaction with two other nonstructural proteins, including Nterm and P22, (iv) the formation of homodimers or homo-oligomers, and (v) the induction of cell apoptosis. Taken together, our findings emphasize that the virus-encoded NTPase must have multiple activities during viral replication or pathogenesis; however, these activities may vary somewhat among different genogroups.
Controlled Activity of the
Salmonella
Invasion-Associated Injectisome Reveals Its Intracellular Role in the Cytosolic Population
Authors: Klein Jessica A. , Grenz Jesse R. , Slauch James M. , Knodler Leigh A. 
Published: December 2017

ABSTRACT

The
Salmonella
invasion-associated type III secretion system (T3SS1) is an essential virulence factor required for entry into nonphagocytic cells and consequent uptake into a
Salmonella
-containing vacuole (SCV). While
Salmonella
is typically regarded as a vacuolar pathogen, a subset of bacteria escape from the SCV in epithelial cells and eventually hyperreplicate in the cytosol. T3SS1 is downregulated following bacterial entry into mammalian cells, but cytosolic
Salmonella
cells are T3SS1 induced, suggesting prolonged or resurgent activity of T3SS1 in this population. In order to investigate the postinternalization contributions of T3SS1 to the
Salmonella
infectious cycle in epithelial cells, we bypassed its requirement for bacterial entry by tagging the T3SS1-energizing ATPase InvC at the C terminus with peptides that are recognized by bacterial tail-specific proteases. This caused a dramatic increase in InvC turnover which rendered even assembled injectisomes inactive. Bacterial strains conditionally expressing these unstable InvC variants were proficient for invasion but underwent rapid and sustained intracellular inactivation of T3SS1 activity when InvC expression ceased. This allowed us to directly implicate T3SS1 activity in cytosolic colonization and bacterial egress. We subsequently identified two T3SS1-delivered effectors, SopB and SipA, that are required for efficient colonization of the epithelial cell cytosol. Overall, our findings support a multifaceted, postinvasion role for T3SS1 and its effectors in defining the cytosolic population of intracellular
Salmonella
.

IMPORTANCE
A needle-like apparatus, the type III secretion system (T3SS) injectisome, is absolutely required for
Salmonella enterica
to enter epithelial cells; this requirement has hampered the analysis of its postentry contributions. To identify T3SS1-dependent intracellular activities, in this study we overcame this limitation by developing a conditional inactivation in the T3SS whereby T3SS activity is chemically induced during culture in liquid broth, permitting bacterial entry into epithelial cells, but is quickly and perpetually inactivated in the absence of inducer. In this sense, the mutant acts like wild-type bacteria when extracellular and as a T3SS mutant once it enters a host cell. This “conditional” mutant allowed us to directly link activity of this T3SS with nascent vacuole lysis, cytosolic proliferation, and cellular egress, demonstrating that the invasion-associated T3SS also contributes to essential intracellular stages of the
S. enterica
infectious cycle.
Trabeculectomy bleb needling and antimetabolite administration practices in the UK: a glaucoma specialist national survey
Authors: Mercieca Karl , Drury Brett , Bhargava Archana , Fenerty Cecilia 
Published: December 2017

AimsTo evaluate, describe and quantify the diversity in postoperative antimetabolite administration and bleb needling practices among glaucoma specialists performing trabeculectomy surgery within the UK and Ireland.MethodsA cross-sectional online survey was distributed to all consultant glaucoma specialists who are on the United Kingdom and Eire Glaucoma Society (UKEGS) contact list. Participants were asked specific questions about their current practices for post-trabeculectomy antimetabolite administration followed by questions directly related to bleb needling procedures.Results60 (83%) of UKEGS glaucoma subspecialty consultants completed the survey. 70% of respondents administered 5-fluorouracil (5-FU) in their clinic room while 30% used a separate treatment room. Doses of 5-FU varied considerably but 70% used 5 mg as standard. Techniques used to reduce corneal toxicity included precipitation with amethocaine (44%) or benoxinate (14%), saline wash (14%) and modified injection technique (8%). Topical antibiotics and/or betadine were used to prevent infection following 5-FU injection in just over 50%. Bleb needling was exclusively performed in operating theatre by 56% of respondents and solely at the slit lamp in the clinic room by 12%. A further 30% used a combination of both theatre and outpatient clinic rooms. Anti-metabolites used were 5-FU (72%) and mitomycin C (22%) with 12% using either of the two substances.ConclusionsThere is a significantly wide variety of current practices for antimetabolite administration and bleb needling within the UK and Ireland. This may be influenced by a glaucoma surgeon’s specific experience and audit results as well as particular clinical set-up, availability of antimetabolite and clinic room space.
‘We lost because of his drunkenness’: the social processes linking alcohol use to self-harm in the context of daily life stress in marriages and intimate relationships in rural Sri Lanka
Authors: Sørensen Jane Brandt , Agampodi Thilini , Sørensen Birgitte Refslund , Siribaddana Sisira , Konradsen Flemming , Rheinländer Thilde 
Published: December 2017

IntroductionHarmful alcohol use has been found to cause detriment to the consumers and those around them. Research carried out in Sri Lanka has described the socioeconomic consequences to families owing to alcohol consumption. However, the social processes around alcohol use and how it could result in behaviour such as self-harm was unclear. With an outset in daily life stressors in marriages and intimate relationships we explored alcohol use in families with a recent case of self-harm.MethodsQualitative data were collected for 11 months in 2014 and 2015 in the North Central and North Western provinces of Sri Lanka. Narrative life story interviews with 19 individuals who had self-harmed where alcohol was involved and 25 of their relatives were conducted. Ten focus group discussions were carried out in gender and age segregated groups. An inductive content analysis was carried out.ResultsParticipants experienced two types of daily life stressors: non-alcohol-related stressors, such as violence and financial difficulties, and alcohol-related stressors. The alcohol-related stressors aggravated the non-alcohol-related daily life stressors within marriages and intimate relationships, which resulted in conflict between partners and subsequent self-harm. Women were disproportionately influenced by daily life stressors and were challenged in their ability to live up to gendered norms of marriage. Further, women were left responsible for their ownandtheir husband’s inappropriate behaviour. Self-harm appeared to be a possible avenue of expressing distress. Gendered alcohol and marriage norms provided men with acceptable excuses for their behaviour, whether it was alcohol consumption, conflicts or self-harm.ConclusionsThis study found that participants experienced both alcohol-related and non-alcohol-related daily life stressors. These two categories of daily life stressors, gender inequalities and alcohol norms should be considered when planning alcohol and self-harm prevention in this setting. Life situations also reflected larger community and structural issues.
Misleading signs in acute vertigo
Authors: Lance Sean , Mossman Stuart Scott 
Published: December 2017

The acute vestibular syndrome is common and usually has a benign cause. Sometimes, however, even experienced neurologists can find it difficult to determine the cause clinically. Furthermore, neuroimaging is known to be insensitive.We describe two cases of acute vestibular syndrome where conflicting clinical findings contributed to a delay in making the correct diagnosis. The first patient with symptomatic vertigo had signs consistent with horizontal benign paroxysmal positional vertigo but also had an abnormal horizontal head impulse test, superficially suggesting acute vestibular neuritis but later accounted for by the finding of a vestibular schwannoma (acoustic neuroma). The second patient also had an abnormal horizontal head impulse test, with skew deviation suggesting stroke as the cause. However, later assessment identified that a long-standing fourth nerve palsy was the true cause for her apparent skew. We discuss potential errors that can arise when assessing such patients and highlight ways to avoid them.
Flows in non-equilibrium quantum systems and quantum photosynthesis
Authors: Kozyrev S. V. , Mironov A. A. , Teretenkov A. E. , Volovich I. V. 
Published: December 2017

A three-level quantum system interacting with non-equilibrium environment is investigated. The stationary state of the system is found (both for non-coherent and coherent environment) and relaxation and decoherence to the stationary state is described. The stationary state of the system will be non-equilibrium and will generate flows. We describe the dependence of the flows on the state of the environment. We also discuss application of this model to the problem of quantum photosynthesis, in particular, to the description of flows of excitons and generation of excitonic coherences.
Editor's Note/Chairs' Welcome
Authors: Karahalios Karrie , Fitzpatrick Geraldine , Monroy-Hernández Andrés 
Published: December 2017

Welcome to this issue of the Proceedings of the ACM on Human-Computer Interaction, which will focus on contributions from the research community Computer-Supported Cooperative Work and Social Computing (CSCW). This diverse research community explores how different types of social groups affect, and are affected by, information and communication technology. The topics explored by this community can include social media use, crowdsourcing and micro-work, societal effects of computing, and much more. Like many other HCI communities, CSCW approaches these topics with a broad range of scientific techniques, theoretical perspectives and technology platforms.
The call for papers for this issue on CSCW attracted 385 submissions, from Asia, Canada, Australia, Europe, Africa, and the United States. After the first round of reviewing, 207 (54%) papers were invited to the Revise and Resubmit phase. The editorial committee worked hard over August 2017 to arrive at final decisions, with a Virtual Committee meeting held to discuss those papers that needed collective deliberation. In the end, 105 papers (27%) were accepted.
This issue exists because of the dedicated volunteer effort of 101 senior editors who served as Associate Chairs (ACs), and 885 expert reviewers to ensure high quality and insightful reviews for all papers in both rounds. Reviewers and committee members were kept constant for papers that submitted to both rounds. Senior members of the editorial group also helped shepherd some papers, reflecting the deep commitment of this research community.
We are excited by the compelling and thought-provoking work that resulted in this PACMHCI CSCW issue and look forward to equally high quality submissions for the next submission cycle from this research community in the Spring of 2018. For those interested in this area, this group holds their next annual conference November 3-7, 2018 in New York City's Hudson River (Jersey City). That conference will provide many opportunities to share ideas with other researchers and practitioners from institutions around the world.
Customization of biliopancreatic limb length to modulate and sustain antidiabetic effect of gastric bypass surgery
Authors: Pal A. , Rhoads D. B. , Tavakkoli A. 
Published: December 2017

Although Roux-en-Y Gastric Bypass (RYGB) remains the most effective treatment for obesity and type 2 diabetes (T2D), many patients fail to achieve remission, or relapse. Increasing intestinal limb lengths of RYGB may improve outcomes, but the mechanistic basis for this remains unclear. We hypothesize biliopancreatic (BP) limb length modulates the antidiabetic effect of RYGB. Rats underwent RYGB with a 20-cm (RYGB-20cm) or 40-cm (RYGB-40cm) BP limb and were compared with control animals. After 2 and 4 wk, portal and systemic blood was sampled during intestinal glucose infusion. Portosystemic gradient was used to calculate intestinal glucose utilization (Gutil), absorption (Gabsorp), and hormone secretion. Intestinal morphology and gene expression were assessed. At 2 wk, Gabsorp progressively decreased with increasing BP limb length; this pattern persisted at 4 wk. Gutil increased ≈70% in both RYGB-20cm and -40cm groups at 2 wk. At 4 wk, Gutil progressively increased with limb length. Furthermore, Roux limb weight, and expression of hexokinase and preproglucagon, exhibited a similar progressive increase. At 4 wk, glucagon-like peptide-1 and -2 levels were higher after RYGB-40cm, with associated increased secretion. We conclude that BP limb length modulates multiple antidiabetic mechanisms, analogous to the dose-response relationship of a drug. Early postoperatively, a longer BP limb reduces Gabsorp. Later, Gutil, Roux limb hypertrophy, hormone secretion, and hormone levels are increased with longer BP limb. Sustained high incretin levels may prevent weight regain and T2D relapse. These data provide the basis for customizing BP limb length according to patient characteristics and desired metabolic effect. NEW & NOTEWORTHY Biliopancreatic limb length in gastric bypass modulates multiple antidiabetic mechanisms, analogous to the dose-response relationship of a drug. With a longer biliopancreatic limb, Roux limb hypertrophy, increased glucose utilization, reduced glucose absorption, and sustained high incretin levels may prevent weight regain and diabetes relapse.
VEGF nanoparticles repair the heart after myocardial infarction
Authors: Oduk Yasin , Zhu Wuqiang , Kannappan Ramaswamy , Zhao Meng , Borovjagin Anton V. , Oparil Suzanne , Zhang Jianyi (Jay) 
Published: December 2017

Vascular endothelial growth factor (VEGF) is a well-characterized proangiogenic cytokine that has been shown to promote neovascularization in hearts of patients with ischemic heart disease but can also lead to adverse effects depending on the dose and mode of delivery. We investigated whether prolonged exposure to a low dose of VEGF could be achieved by encapsulating VEGF in polylactic coglycolic acid nanoparticles and whether treatment with VEGF-containing nanoparticles improved cardiac function and protected against left ventricular remodeling in the hearts of mice with experimentally induced myocardial infarction. Polylactic coglycolic acid nanoparticles with a mean diameter of ~113 nm were generated via double emulsion and loaded with VEGF; the encapsulation efficiency was 53.5 ± 1.7% (107.1 ± 3.3 ng VEGF/mg nanoparticles). In culture, VEGF nanoparticles released VEGF continuously for at least 31 days, and in a murine myocardial infarction model, VEGF nanoparticle administration was associated with significantly greater vascular density in the peri-infarct region, reductions in infarct size, and improvements in left ventricular contractile function 4 wk after treatment. Thus, our study provides proof of principle that nanoparticle-mediated delivery increases the angiogenic and therapeutic potency of VEGF for the treatment of ischemic heart disease. NEW & NOTEWORTHY Vascular endothelial growth factor (VEGF) is a well-characterized proangiogenic cytokine but has a short half-life and a rapid clearance rate. When encapsulated in nanoparticles, VEGF was released for 31 days and improved left ventricular function in infarcted mouse hearts. These observations indicate that our new platform increases the therapeutic potency of VEGF.