bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between dynapenic obesity phenotypes and physical performance in middle-age and older women living in community
Authors: Arteaga-Pazmiño Cecilia , Fonseca-Pérez Diana , Balladares Mazzini Manuel , Galvez-Celi Javier , Emén Sánchez Janet , Álvarez-Córdova Ludwig 
Published: September 2024

BackgroundDynapenic obesity (DO) is the coexistence of excess adipose tissue/body weight and low muscle strength. This condition is associated with an increased risk of suffering from various chronic diseases and physical deterioration in older people.AimTo analyze the association between DO phenotypes and physical performance in middle-aged women living in the community.MethodsThis cross-sectional study was conducted on middle-aged and older women (≥50 years) residing in Guayaquil, Ecuador. Dynapenia was diagnosticated by a handgrip strength (HGS) &lt; 16 kg; obesity was determined based on body mass index (BMI) ≥ 30 kg/m2. Participants were categorized into four groups based on their dynapenia and obesity status: non-dynapenic/non-obesity (ND/NO), obesity/non-dynapenic (O/ND), dynapenic/non-obesity (D/NO) and dynapenic/obesity (D/O). Physical performance was assessed by the Short Physical Performance Battery (SPPB).ResultsA total of 171 women were assessed. The median (IQR) age of the sample was 72.0 (17.0) years. Obesity and dynapenia were 35% (n = 60) and 57.8% (n = 99) of the participants, respectively. The prevalence of ND/NO was 25.1% (n = 43), O/ND 17% (n = 29), D/NO 39.8% (n = 68) and DO 18.1% (n = 31). The mean SPPB total score was 6.5 ± 3.2. Participants of D/NO and DO groups presented significantly lower mean SPPB scores (p &lt; 0.001) compared to those of NO/ND and O/ND groups.ConclusionWomen with DO and D/NO exhibited significantly lower SPPB scores, indicating poorer physical performance. These findings emphasize the importance of incorporating a comprehensive assessment of muscle strength and obesity in middle-aged and older women.
ASPYRE-Lung: validation of a simple, fast, robust and novel method for multi-variant genomic analysis of actionable NSCLC variants in FFPE tissue
Authors: Evans Ryan T. , Gillon-Zhang Elizabeth , Brown Julia N. , Knudsen Katherine E. , King Candace , Green Amanda S. , Silva Ana-Luisa , Mordaka Justyna M. , Palmer Rebecca N. , Tomassini Alessandro , Collazos Alejandra , Xyrafaki Christina , Turner Iyelola , Ho Chau Ha , Nugent Dilyara , Jose Jinsy , Andreazza Simonetta , Potts Nicola D. , von Bargen Kristine , Gray Eleanor R. , Stolarek-Januszkiewicz Magdalena , Cooke Aishling , Reddi Honey V. , Balmforth Barnaby W. , Osborne Robert J. 
Published: September 2024

IntroductionGenomic variant testing of tumors is a critical gateway for patients to access the full potential of personalized oncology therapeutics. Current methods such as next-generation sequencing are costly and challenging to interpret, while PCR assays are limited in the number of variants they can cover. We developed ASPYRE® (Allele-Specific PYrophosphorolysis REaction) technology to address the urgent need for rapid, accessible and affordable diagnostics informing actionable genomic target variants of a given cancer. The targeted ASPYRE-Lung panel for non-small cell carcinoma covers 114 variants in 11 genes (ALK, BRAF, EGFR, ERBB2, KRAS, RET, ROS1, MET &amp; NTRK1/2/3) to robustly inform clinical management. The assay detects single nucleotide variants, insertions, deletions, and gene fusions from tissue-derived DNA and RNA simultaneously.MethodsWe tested the limit of detection, specificity, analytical accuracy and analytical precision of ASPYRE-Lung using FFPE lung tissue samples from patients with non-small cell lung carcinoma, variant-negative FFPE tissue from healthy donors, and FFPE-based contrived samples with controllable variant allele fractions.ResultsThe sensitivity of ASPYRE-Lung was determined to be ≤ 3% variant allele fraction for single nucleotide variants and insertions or deletions, 100 copies for fusions, and 200 copies for MET exon 14 skipping. The specificity was 100% with no false positive results. The analytical accuracy test yielded no discordant calls between ASPYRE-Lung and expected results for clinical samples (via orthogonal testing) or contrived samples, and results were replicable across operators, reagent lots, runs, and real-time PCR instruments with a high degree of precision.ConclusionsThe technology is simple and fast, requiring only four reagent transfer steps using standard laboratory equipment (PCR and qPCR instruments) with analysis via a cloud-based algorithm. The ASPYRE-Lung assay has the potential to be transformative in facilitating access to rapid, actionable molecular profiling of tissue for patients with non-small cell carcinoma.
Transumbilical single-site laparoscopic treatment of primary splenic cyst in child: a rare case report and review of literature
Authors: Kong Meng , Chen Shuai , Bai Yuexia , Yan Yuxi , Yi Minggang , Wang Meiyun , Liu Hongzhen , Jia Jinhua , Liu Chuanyang , Zhang Shisong 
Published: September 2024

BackgroundSplenic cysts are relatively rare benign tumors that are asymptomatic in most patients and are usually discovered incidentally by imaging. In our case, we report a splenic cyst in a child who underwent laparoscopic partial splenectomy.Case descriptionA 13-year-old boy was admitted to the hospital after an incidental finding of a splenic cyst on an abdominal ultrasound conducted 4 days prior. He was asymptomatic before admission. Upon admission, abdominal ultrasound and CT revealed a cystic lesion in the spleen, highly suspicious for a splenic cyst. Then, we used transumbilical single-site laparoscopic exploration and found a cyst measuring approximately 12 cm × 11 cm × 10 cm at the upper pole of the spleen, so we performed a partial splenectomy and diagnosed a primary epithelioid splenic cyst via postoperative pathology.ConclusionsSplenic cysts in children are very rare and can be treated conservatively in asymptomatic patients with a diameter of less than 5 cm, while surgery is required in symptomatic patients or those with a diameter greater than or equal to 5 cm. Transumbilical single-site laparoscopic partial splenectomy is a minimally invasive and effective treatment, especially for children.
Neuroprotective effects of Gastrodia elata Blume on promoting M2 microglial polarization by inhibiting JNK/TLR4/T3JAM/NF-κB signaling after transient ischemic stroke in rats
Authors: Huang Shang-Chih , Huang Hui-Chi , Liao Wen-Ling , Kao Shung-Te , Cheng Chin-Yi 
Published: September 2024

BackgroundGastrodia elata Blume, also called Tian Ma (TM), has been used to treat stroke for centuries. However, its effects on inflammation in acute cerebral ischemic injury and underlying mechanisms involved in microglial polarization remain unknown. The present study explored the effects of the TM extract on the modulation of microglial M1/M2 polarization 2 days after transient cerebral ischemia.MethodsMale Sprague Dawley rats were intracerebroventricularly administered with 1% dimethyl sulfoxide 25 min before cerebral ischemia and subsequently intraperitoneally administered 0.25 g/kg (DO + TM-0.25 g), 0.5 g/kg (DO + TM-0.5 g), or 1 g/kg (DO + TM-1 g) of the TM extract after cerebral ischemia onset.ResultsDO + TM-0.5 g and DO + TM-1 g treatments downregulated the following: phospho-c-Jun N-terminal kinase (p-JNK)/JNK, tumor necrosis factor (TNF) receptor-associated factor 3 (TRAF3), TRAF3-interacting JNK-activating modulator (T3JAM), p-nuclear factor-kappa B p65 (p-NF-κB p65)/NF-κB p65, ionized calcium-binding adapter molecule 1 (Iba1), CD86, TNF-α, interleukin (IL)-1β, and IL-6 expression and toll-like receptor 4 (TLR4)/Iba1, CD86/Iba1, and p-NF-κB p65/Iba1 coexpression. These treatments also upregulated IL-10, nerve growth factor, and vascular endothelial growth factor A expression and YM-1/2/Iba1 and IL-10/neuronal nuclei coexpression in the cortical ischemic rim. The JNK inhibitor SP600125 exerted similar treatment effects as the DO + TM-0.5 g and DO + TM-1 g treatments.ConclusionDO + TM-0.5 g and DO + TM-1 g/kg treatments attenuate cerebral infarction by inhibiting JNK-mediated signaling. TM likely exerts the neuroprotective effects of promoting M1 to M2 microglial polarization by inhibiting JNK/TLR4/T3JAM/NF-κB-mediated signaling in the cortical ischemic rim 2 days after transient cerebral ischemia.
Malva sylvestris leaf powder as a feed additive affects the performance, carcass traits, meat quality attributes, serum antioxidants, stress physiology, intestinal bacterial counts, and gut morphology of broiler chicken
Authors: Banday Mohammad T. , Wani Manzoor A. , Alqhtani Haifa A. , Bin-Jumah May , Rudayni Hassan A. , Allam Ahmed A. , Algopishi Uthman Balgith , Adil Sheikh 
Published: September 2024

This study investigated the effect of supplementation of Malva sylvestris leaf powder (MSLP) on the production performance of broiler chicken. Ven Cobb broiler chicks (240 day-old male chicks) were distributed randomly into four treatments, each replicated four times, with 15 birds per replicate. The diets formulated were T1 (control) given basal diet only, T2 (basal diet +1.0% MSLP), T3 (basal diet +1.5% MSLP), and T4 (basal diet +2.0% MSLP). The highest improvement of 3.83% in the average daily gain (ADG) was recorded in the T3 group fed 1.5% Malva powder in the diet compared to the control (P = 0.009). The average daily feed intake (ADFI) tended to decrease with an increase in the dose of MSLP in the diet, with the lowest feed intake in the T4 group fed 2% MSLP. During the overall period (7–42 days), the feed/gain (F/G) ratio reduced significantly (P = 0.048) in the T3 and T4 groups compared to the control. The dressed and breast meat yield was found to be significantly (P &lt; 0.05) higher in the T3 group, with no significant change (P &gt; 0.05) in the thigh yield. The changes in the pH and water-holding capacity (WHC) of breast meat were found to be non-significant (p &gt; 0.05) between the control and various other treatments. Thiobarbituric acid-reactive substances (TBARS) were significantly (P &lt; 0.05) decreased in the T3 and T4 groups. There was no negative effect of including MSLP in the diet on the color coordinates of breast meat among different treatments. Compared to the control, the serum immunoglobulin values increased significantly (P &lt; 0.05) in the T3 and T4 groups. Superoxide dismutase (SOD) showed no difference between various treatments; however, malondialdehyde (MDA) decreased significantly (P &lt; 0.05) according to dietary treatments. Serum cortisol increased significantly (P &lt; 0.05) in the T4 group compared to other treatments. The inclusion of Malva powder in the diet at the 2% level significantly (P &lt; 0.05) decreased the coliform count compared to the control birds. Supplementation with Malva powder resulted in a significant (P &lt; 0.05) increase in the villus height-to-crypt depth (VH:CD) ratio of broiler birds in the T3 and T4 groups. In conclusion, MSLP supplementation at 1.5% and 2% resulted in improved production performance of broiler chicken.
The effects of applying cellulase and laccase on fermentation quality and microbial community in mixed silage containing corn stover and wet brewer’s grains
Authors: Li Li , Xie Xiangxue , Zhao Guoqiang , He Jiajun , Zhang Yongliang 
Published: September 2024

ObjectiveThe purpose of this experiment was to explore the effect of adding cellulase and laccase on fermentation quality and microbial community in mixed silage of corn stover and wet brewer’s grains. Try to a new approach for the proper preservation and utilization of the agro-industrial by-products (corn stover and wet brewer’s grains).MethodsThe experiment was divided into four groups: CK (control), C (cellulase, 120 U/g fresh matter [FM]), L (laccase, 50 U/g FM), CL (cellulase 120 U/g FW and laccase 50 U/g FM), and the chemical composition, fermentation quality, microbial population and microbial community in mixed silage of corn stover and wet brewer’s grains after 30 day’s fermentation were determined.ResultsCompared to control, the addition of cellulase significantly increased crude protein (CP), water-soluble carbohydrate (WSC), lactic acid bacteria (LAB) counts, while significantly decreased the neutral detergent fiber (NDF), acid detergent fiber (ADF) content (P &lt; 0.05). Adding laccase significantly decreased the acid detergent lignin (ADL) content (P &lt; 0.05). Combined application of cellulase and laccase significantly increased the CP, WSC content and LAB counts, while significantly decreased pH value, NDF, ADF and ADL content (P &lt; 0.05), thereby improving fermentation quality. In addition, the application of cellulase and laccase increased the abundance of Firmicutes and LAB genera, and decreased microbial diversity level of the sample.ConclusionThe combined application of cellulase and laccase further improved fermentation quality and microbial community in mixed silage of corn stover and wet brewer’s grains.
How depression and ADHD relate to exercise addiction: a cross-sectional study among frequent exercisers
Authors: Baltes-Flueckiger Lavinia , Wagner Aline , Sattler Isabel , Meyer Maximilian , Tschopp Amos , Walter Marc , Colledge Flora 
Published: September 2024

BackgroundTo date, there are no official diagnostic criteria for the frequently reported phenomenon of exercise addiction. Therefore, the aim of the present study was to investigate how mental disorders, specifically depression and attention-deficit hyperactivity disorder (ADHD), are related to exercise addiction (EA).MethodsA total of 173 participants aged between 18 and 70 years, who reported exercising more than 10 h a week and continued to exercise despite injury or illness, answered questionnaires including the Exercise Dependence Scale, the Beck Depression Inventory, and the Homburger ADHD scale for adults. Multiple linear regression analyses were performed adjusting for relevant confounders (age, gender) and stepwise regression was used to identify which of the two mental disorders is the more influential predictor of EA.ResultsPearson correlation analysis showed that depressive symptoms [r (171) = 0.422, p &lt; 0.00] and ADHD symptoms [r (171) = 0.308, p &lt; 0.001] were positively correlated with EA symptoms. The relation between depressive symptoms and EA remained after adjusting for confounders in the regression model (B = 20.531; t(170) = 5.950; 95% CI [13.719, 27.343]; p &lt; 0.001). Similarly, the positive link between ADHD symptoms and EA persisted after controlling for confounders (B = 15.507; t(170) = 3.771; 95% CI [7.389, 23.625]; p &lt; 0.001). Additionally, a stepwise regression model identified that depressive symptoms are a stronger predictor for EA than ADHD symptoms.ConclusionDepressive symptoms seem to be a stronger predictor for EA compared to ADHD symptoms in frequent exercisers. Although individuals with ADHD May exercise extensively, they might be less at risk for EA than individuals with depression. These results contribute to the complex characterization of the psychiatric profile of individuals with exercise addiction, and underline the need for further research elucidating the interplay between mental disorders and EA.
Economic evaluation of acupuncture in treating patients with pain and mental health concerns: the results of the Alberta Complementary Health Integration Project
Authors: Lu Mingshan , Sharmin Sumaiya , Tao Yong , Xia Xin , Yang Gongliang , Cong Yingying , Yang Guanhu , Razavilar Negar , Aziz Riffat , Jiang Jing , Xiao Yun , Peng Laura , Xu Bentong 
Published: September 2024

BackgroundThe COVID-19 pandemic and its economic impact have heightened the risk of mental health and pain-related issues. The integration of acupuncture with conventional medicine shows promise in improving treatment outcomes for these conditions. The Alberta Complementary Health Integration Project (ABCHIP) aimed to provide acupuncture to youth (aged 24 and under) and seniors (aged 55 and above) experiencing chronic pain, pain management issues, mental health issues, and/or related conditions. The program aimed to promote integrative care, assess the effectiveness and cost-effectiveness of these therapies, and deliver patient-centered care.DesignABCHIP provided acupuncture to address pain, mental health, and addiction issues at no cost to two vulnerable populations in Alberta: youth and the older adult. A total of 606 patients aged 14–65 received 5,424 acupuncture treatments. Outcome measures included pain interference, pain severity, sleep quality, depression, anxiety, fatigue, anger, and quality of life. Short-term outcomes were assessed through questionnaires completed at the beginning and completion of the treatments, while long-term benefits were estimated using these outcome indicators and existing literature on the economic cost of illnesses.ResultThe cost-effectiveness analysis revealed the following ratios per Quality-Adjusted Life Year (QALY): CND12,171 for the overall sample, CND10,766 for patients with pain, CND9,331 for individuals with depression, and CND9,030 for those with anxiety. The cost–benefit analysis demonstrated annual cost savings ranging from CND1,487 to CND5,255, with an average of CND3,371.ConclusionThe study findings indicate that ABCHIP’s treatment for pain, depression, anxiety, and sleep issues is cost-effective, leading to substantial cost savings and improved quality of life for patients. The program’s cost per Quality-Adjusted Life Year (QALY) is significantly lower than benchmarks used in other countries, demonstrating high cost-effectiveness and value. Patients receiving 12 treatments experienced significant improvements across all measures, with estimated economic benefits surpassing treatment costs. In summary, ABCHIP offers a cost-effective and economically efficient therapy choice for individuals dealing with pain and mental health issues.
Radon exposure and potential health effects other than lung cancer: a systematic review and meta-analysis
Authors: Henyoh Afi Mawulawoe Sylvie , Laurent Olivier , Mandin Corinne , Clero Enora 
Published: September 2024

Context and objectiveTo date, lung cancer is the only well-established health effect associated with radon exposure in humans. To summarize available evidence on other potential health effects of radon exposure, we performed a comprehensive qualitative and quantitative synthesis of the available literature on radon exposure and health effects other than lung cancer, in both occupational and general populations.MethodEligible studies published from January 1990 to March 2023, in English and French languages, were identified in PubMed, ScienceDirect, Scopus, ScieLo and HAL. In the meta-analysis, we estimated average weighted standardized incidence ratios (metaSIR), standardized mortality ratios (metaSMR), and risk ratio (metaRR) per 100 unit (Bq/m3 or Working level Month) increase in radon exposure concentration by combining estimates from the eligible studies using the random-effect inverse variance method. DerSimonian &amp; Laird estimator was used to estimate the between-study variance. For each health outcome, analyses were performed separately for mine workers, children, and adults in the general population.ResultsA total of 129 studies were included in the systematic review and 40 distinct studies in the meta-analysis. For most of these health outcomes, the results of the meta-analyses showed no statistically significant association, and heterogeneity was only present among occupational studies, especially between those included in the metaSIR or metaSMR analyses. However, the estimated exposure-risk associations were positive and close to the statistical significance threshold for: lymphohematological cancer incidence in children (metaRR = 1.01; 95%CI: 1.00–1.03; p = 0.08); malignant melanoma mortality among adults in the general population (metaRR = 1.10; 95%CI: 0.99–1.21; p = 0.07); liver cancer mortality among mine workers (metaRR = 1.04; 95%CI: 1.00–1.10; p = 0.06); intestine and rectal cancer mortality combined among mine workers (metaRR = 1.02; 95%CI: 1.00–1.04; p = 0.06).ConclusionAlthough none of the exposure-risk associations estimated in the meta-analyses reached statistical significance, the hypothesis that radon may have other health effects apart from lung cancer could not be ruled-out and call for additional research. Larger and well-designed studies are needed to further investigate this question.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023474542, ID: CRD42023474542.
Developmental evaluation of the healthy futures of Texas’ puberty curriculum: On My Way
Authors: Schormann Elizabeth , Betori Anthony , Neubauer Leah C. 
Published: September 2024

BackgroundThis paper describes a developmental evaluation (DE) of a pilot of a puberty curriculum that was implemented in grades four to six in San Antonio, Texas. The pilot evaluation assessed the initial feasibility and acceptability of curricular components. The DE framework guided the questions in an ever-changing environment where new tools were created as the situation called for them (10).MethodsThe evaluation team utilized purposive sampling methods, surveys, and facilitator notes to guide the collaborative process. Both Google and Microsoft platforms were used for analysis and collection of findings.ResultsFacilitator notes and surveys revealed that while comfortable leading sessions, there were still issues in timing and student comprehension. From a student’s point of view, while many (60%) reported feeling uncomfortable during lessons, a greater majority (80%) reported learning something from the sessions.ConclusionDE was a crucial piece of the pilot sessions and revision process despite any limitations. A user-focused and adaptable evaluation generated greater opportunities for positive change within the curriculum and its delivery.