bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene
Authors: Pink Matthias , Ratsch Boris A. , Mardahl Maibritt , Durek Pawel , Polansky Julia K. , Karl Martin , Baumgrass Ria , Wallner Stefan , Cadenas Cristina , Gianmoena Kathrin , Floess Stefan , Chen Wei , Nordstroem Karl , Tierling Sascha , Olek Sven , Walter Jörn , Hamann Alf , Syrbe Uta 
Published: September 2016

Abstract
E- and P-selectin ligands (E- and P-ligs) guide effector memory T cells into skin and inflamed regions, mediate the inflammatory recruitment of leukocytes, and contribute to the localization of hematopoietic precursor cells. A better understanding of their molecular regulation is therefore of significant interest with regard to therapeutic approaches targeting these pathways. In this study, we examined the transcriptional regulation of fucosyltransferase 7 (FUT7), an enzyme crucial for generation of the glycosylated E- and P-ligs. We found that high expression of the coding gene fut7 in murine CD4+ T cells correlates with DNA demethylation within a minimal promoter in skin/inflammation-seeking effector memory T cells. Retinoic acid, a known inducer of the gut-homing phenotype, abrogated the activation-induced demethylation of this region, which contains a cAMP responsive element. Methylation of the promoter or mutation of the cAMP responsive element abolished promoter activity and the binding of CREB, confirming the importance of this region and of its demethylation for fut7 transcription in T cells. Furthermore, studies on human CD4+ effector memory T cells confirmed demethylation within FUT7 corresponding to high FUT7 expression. Monocytes showed an even more extensive demethylation of the FUT7 gene whereas hepatocytes, which lack selectin ligand expression, exhibited extensive methylation. In conclusion, we show that DNA demethylation within the fut7 gene controls selectin ligand expression in mice and humans, including the inducible topographic commitment of T cells for skin and inflamed sites.
Circulating Memory CD4+ T Cells Target Conserved Epitopes of Rhinovirus Capsid Proteins and Respond Rapidly to Experimental Infection in Humans
Authors: Muehling Lyndsey M. , Mai Duy T. , Kwok William W. , Heymann Peter W. , Pomés Anna , Woodfolk Judith A. 
Published: September 2016

Abstract
Rhinovirus (RV) is a major cause of common cold and an important trigger of acute episodes of chronic lung diseases. Antigenic variation across the numerous RV strains results in frequent infections and a lack of durable cross-protection. Because the nature of human CD4+ T cells that target RV is largely unknown, T cell epitopes of RV capsid proteins were analyzed, and cognate T cells were characterized in healthy subjects and those infected by intranasal challenge. Peptide epitopes of the RV-A16 capsid proteins VP1 and VP2 were identified by peptide/MHC class II tetramer-guided epitope mapping, validated by direct ex vivo enumeration, and interrogated using a variety of in silico methods. Among noninfected subjects, those circulating RV-A16–specific CD4+ T cells detected at the highest frequencies targeted 10 unique epitopes that bound to diverse HLA-DR molecules. T cell epitopes localized to conserved molecular regions of biological significance to the virus were enriched for HLA class I and II binding motifs, and constituted both species-specific (RV-A) and pan-species (RV-A, -B, and -C) varieties. Circulating epitope-specific T cells comprised both memory Th1 and T follicular helper cells, and were rapidly expanded and activated after intranasal challenge with RV-A16. Cross-reactivity was evidenced by identification of a common *0401-restricted epitope for RV-A16 and RV-A39 by tetramer-guided epitope mapping and the ability for RV-A16–specific Th1 cells to proliferate in response to their RV-A39 peptide counterpart. The preferential persistence of high-frequency RV-specific memory Th1 cells that recognize a limited set of conserved epitopes likely arises from iterative priming by previous exposures to different RV strains.
Boosting of ALVAC-SIV Vaccine-Primed Macaques with the CD4-SIVgp120 Fusion Protein Elicits Antibodies to V2 Associated with a Decreased Risk of SIVmac251 Acquisition
Authors: Gordon Shari N. , Liyanage Namal P. M. , Doster Melvin N. , Vaccari Monica , Vargas-Inchaustegui Diego A. , Pegu Poonam , Schifanella Luca , Shen Xiaoying , Tomaras Georgia D. , Rao Mangala , Billings Erik A. , Schwartz Jennifer , Prado Ilia , Bobb Kathryn , Zhang Wenlei , Montefiori David C. , Foulds Kathryn E. , Ferrari Guido , Robert-Guroff Marjorie , Roederer Mario , Phan Tran B. , Forthal Donald N. , Stablein Donald M. , Phogat Sanjay , Venzon David J. , Fouts Timothy , Franchini Genoveffa 
Published: September 2016

Abstract
The recombinant ALVAC vaccine coupled with the monomeric gp120/alum protein have decreased the risk of HIV and SIV acquisition. Ab responses to the V1/V2 regions have correlated with a decreased risk of virus acquisition in both humans and macaques. We hypothesized that the breadth and functional profile of Abs induced by an ALVAC/envelope protein regimen could be improved by substituting the monomeric gp120 boost, with the full-length single-chain (FLSC) protein. FLSC is a CD4-gp120 fusion immunogen that exposes cryptic gp120 epitopes to the immune system. We compared the immunogenicity and relative efficiency of an ALVAC-SIV vaccine boosted either with bivalent FLSC proteins or with monomeric gp120 in alum. FLSC was superior to monomeric gp120 in directing Abs to the C3 α2 helix, the V5 loop, and the V3 region that contains the putative CCR5 binding site. In addition, FLSC boosting elicited significantly higher binding Abs to V2 and increased both the Ab-dependent cellular cytotoxicity activity and the breadth of neutralizing Abs. However, the FLSC vaccine regimen demonstrated only a trend in vaccine efficacy, whereas the monomeric gp120 regimen significantly decreased the risk of SIVmac251 acquisition. In both vaccine regimens, anti-V2 Abs correlated with a decreased risk of virus acquisition but differed with regard to systemic or mucosal origin. In the FLSC regimen, serum Abs to V2 correlated, whereas in the monomeric gp120 regimen, V2 Abs in rectal secretions, the site of viral challenge, were associated with efficacy.
The Development and Validity of a Chinese Version of the Compound Remote Associates Test
Authors: Shen Wangbing , Yuan Yuan , Liu Chang , Yi Baoshu , Dou Kai 
Published: September 2016

Abstract
Numerous recent studies have used neuroscientific methods such as event-related potentials and functional magnetic resonance imaging to demystify insight and creativity. To do so, 1 key prerequisite is the creation of a large enough number of homogeneous problems that can reliably produce insight-like experiences within a short time window. The Remote Associates Test (RAT) and its variant, the compound remote associate (CRA) problems developed by Bowden and Jung-Beeman (2003), are 2 of the most popular and important instruments for unraveling the behavioral and cognitive, especially electrophysiological and neural, mechanisms of creative thinking and insight. However, little research has examined cognitive, neural, or even behavioral correlates of remote association and creative insight in the Chinese context because of the absence of a Chinese RAT/CRA. The present work, based on Mednick’s (1962) associative theory, developed a Chinese version of the CRA test with enough items. The reliability, criterion-related validity, and underlying structure of this test were then further assessed. The results revealed that the test possesses satisfactory psychometric properties and is an appropriate psychometric instrument for uncovering neural correlates of creative thinking, creative insight, and associative thought.
The Effect of Collective Transitions on the Organization and Contents of Autobiographical Memory: A Transition Theory Perspective
Authors: Brown Norman R. , Schweickart Oliver , Svob Connie 
Published: September 2016

Abstract
In this article, we first outline a minimalist approach to the organization of autobiographical memory called transition theory. This theory assumes that the content and organization of autobiographical memory mirror the structure of experience and reflect the operation of basic memory processes. Thus, this approach rests on an analysis of the environment that emphasizes repetition, co-occurrence, change, and distinctiveness. We then report a study that tested a set of predictions derived from transition theory. The predictions concerned both the temporal distribution of memorable personal events and the use of public events and historical periods to date those events. To test these predictions, we collected word-cued memories, event-dating protocols, and historical relatedness ratings from 2 groups of Bosnians; on average, people in the younger group were in their early 40s at the outset of the Siege of Sarajevo (1992); those in the older group were in their mid-50s when they experienced this collective transition. As predicted, participants in both groups produced a robust living-in-history effect, often (∼25%) referring to the civil war or the Siege of Sarajevo when dating event memories. They also displayed an upheaval bump, recalling more events from the war years than from prewar and postwar years, and a reminiscence bump, recalling more events from late adolescence and early adulthood than from earlier or later periods. Finally, this study demonstrated, for the first time, the existence of a before/after effect. Specifically, participants often mentioned the war when dating historically unrelated events for the prewar and postwar years. We conclude by considering extensions of transition theory and the significance of our findings for existing models of autobiographical memory.
Adolescent Aggression as Predicted from Parent–Child Relationships and Executive Functions
Authors: Fatima Shameem , Sheikh Hamid 
Published: September 2016

Abstract
Previous research has emphasized the role of parent–child relationships (PCRs) in child and adolescent development. The present study extends the previous findings by examining the direct and mediated relationship between PCRs, executive functioning (EF), and adolescent aggression. Five hundred twelve adolescents of South Asian ethnic background, enrolled at the secondary and higher secondary levels (aged 13–19 years; 50% boys), participated in the study. The Parent–Child Relationship Scale (Rao, 2000), Aggression Scale (Mathur & Bhatnagar, 2004), and four tests from the Delis–Kaplan Executive Function System (Delis, Kaplan, & Kramer, 2001) were administered to measure the perceived quality of PCR, level of aggression, and EF, respectively, in participants. Pearson correlation coefficients revealed that perceived PCRs were related to EF and adolescent aggression among South Asian youth. Furthermore, multiple regression analyses using Baron and Kenny’s (1986) guidelines showed that the influence of PCRs on aggression was partially mediated by EF. The findings suggest that PCRs and EF can be important factors to focus on in interventions aimed at preventing adolescent aggression in society.
Child and Ancient Man: How to Define Their Commonalities and Differences
Authors: Oesterdiekhoff Georg W. 
Published: September 2016

Abstract
Developmental psychology is not only a psychology of development from childhood to old age but a psychology of human development in world history. Eighty years of cross-cultural empirical research findings indicate that the adolescent stage of formal operations evolved late in history and is not a universal development of adult humans across cultures and history. Correspondingly, preoperational or concrete operational stages describe adult psychological stages in past or premodern cultures, as Jean Piaget and some of his followers have mentioned. Developmental psychology is likewise a historical or anthropological psychology capable of describing humans in premodern cultures. The article develops a general anthropological or psychological theory answering the many questions that arise from the correspondences between (modern) children and ancient adults. On this psychological basis, the new structural genetic theory program is capable of explaining, better than previous approaches, the history of humankind from prehistory through ancient to modern societies, the history of economy, society, culture, religion, philosophy, sciences, morals, and everyday life. The accomplishment of this task was once demanded of some classical founders of psychology, sociology, history, and ethnology but was largely avoided by the postwar generations of authors for political and ideological reasons.
Composition and Improvisation in Instrumental Methods Courses: Instrumental Music Teacher Educators’ Perspectives
Authors: Stringham David A. , Thornton Linda C. , Shevock Daniel J. 
Published: September 2016

Abstract
Many musical traditions value creative music making in the form of composition and improvisation. However, research indicates American public school teachers consider improvisation and composition among the least important and most difficult skills to teach. Because instrumental methods courses serve as one source for preparing future instrumental teachers, this mixed methods study elicited experiences, values, and decisions from a national population (N = 321) of instrumental methods instructors. The results of the national survey and interviews with selected participants (n = 8) served as the data sources. Results indicated general support for improvisation and composition in teacher preparation, but low levels of prioritization in instrumental methods courses. Instructors’ comfort with those skills, curricular space, and preparation for existing jobs were among reported barriers to prioritizing composition and improvisation in coursework. Recommendations include greater intentionality in planning for composition and improvisation by teacher educators, professional development opportunities for in-service teachers, and encouraging future music teachers to seek musical experiences beyond typical requirements.
Measuring Music Teacher Identity: Self-Efficacy and Commitment Among Music Teachers
Authors: Wagoner Cynthia L. 
Published: September 2016

Abstract
The purpose of the study was to design an instrument to measure constructs of music teacher identity. The Music Teacher Identity Scale (MTIS), a researcher-constructed data gathering instrument, was developed to measure two constructs of music teacher identity: Music Teacher Self-Efficacy and Music Teacher Commitment. Participants in the study were selected using a stratified sampling technique, based on music teaching level, from the National Association for Music Education’s division membership roster. Descriptive statistics, Cronbach’s coefficient alpha, item-total corrected correlation, and inter-item correlation were used to evaluate the degree of reliability of the survey instrument. An exploratory factor analysis using a direct oblimin rotation was calculated to allow for correlated factors to be considered as subdimensions of the common theme of professional identity and provide evidence of construct validity for the measurement instrument. The MTIS had an overall reliability of α = .81, with the individual constructs of Music Teacher Self-Efficacy reliability of α = .87 and Music Teacher Commitment of α = .67. The two constructs were found to contribute 42.17% of the variance through the exploratory factor analysis. The MTIS has initially been found to be a reliable and valid instrument for measuring Music Teacher Self-Efficacy and Music Teacher Commitment, explaining nearly half of the total variance of contributors to music teacher identity construction. Recommendations are suggested for future research regarding music teacher identity construction utilizing the findings in this study.
“It’s Much More Collaborative”: Democratic Action in Contemporary Collegiate A Cappella
Authors: Berglin Jacob 
Published: September 2016

Abstract
The purpose of this case study was to explore the democratic practices of an all-female contemporary collegiate a cappella ensemble. Due to advances in the Internet and portrayals in popular media, contemporary a cappella participation on collegiate campuses has seen a period of extreme growth. There is interest within music education research to understand the role of democracy in a variety of musical settings, but empirical research connecting democratic action to the everyday practice of music ensembles is relatively sparse. This case study involved documenting and examining 8 hours of observation and 10 hours of group and individual interviews over the course of a 10-week rehearsal and performance sequence. Tan’s (2014) 5 qualities of a democratically run instrumental music ensemble (people, participation, equality, cooperation, and conflict) served as a framework for analysis. Emergent themes included “stepping up,” vulnerability, and transmission. Findings indicate that Tan’s qualities may need reconceptualization in order to apply to a peer-led ensemble working democratically, with equality of opportunity as a primary characteristic. Suggestions for further research and implications for teaching are provided.
KEI en wat er (niet) verandert in het procesrecht in het civiele hoger beroep
Authors: Lock F.J.P. 
Published: September 2016

KEI en wat er (niet) verandert in het procesrecht in het civiele hoger beroep
Dit artikel beoogt in kaart te brengen wat er met de invoering van het programma Kwaliteit en Innovatie rechtspraak (KEI) en de daarbij horende wijzigingen in het Wetboek van Burgerlijke Rechtsvorderingen al dan niet verandert in het appelprocesrecht.
Vindt de consumentenbescherming haar grens in het appelprocesrecht?
Authors: Nieuwenhuijs M. , Möhring M.E.A. 
Published: September 2016

Vindt de consumentenbescherming haar grens in het appelprocesrecht? Gedachten naar aanleiding van HR 26 februari 2016
De Hoge Raad heeft op 26 februari 2016 nader uitgewerkt in welke gevallen de appelrechter ambtshalve moet toetsen. In dit artikel wordt ingegaan op de begrippen “het door de grieven ontsloten gebied” en “de grenzen van de rechtsstrijd”. Daarnaast wordt nagegaan of de lijn van de Hoge Raad strookt met het EU-recht en of verbeteringen in het Nederlandse systeem mogelijk zijn.
Comparative Study of PVC-Free All-Solid-State, PVC Membrane, and Carbon Paste Ion-Selective Electrodes for the Determination of Dapoxetine Hydrochloride in Pharmaceutical Formulation
Authors: Aziz Azza , Khamees Nesrin , Mohamed Tagreed Abdel-Fattah , Derar Abeer Rashad 
Published: September 2016

Abstract
The potentiometric response characteristics and analytical applications of a poly(vinyl chloride) (PVC)-free all-solid-state ion-selective electrode for dapoxetine hydrochloride (DAP) are examined. The Nernstian response of the electrode was evaluated by comparison with PVC-based liquid membrane and carbon paste electrodes. The PVC-free electrode is prepared by direct incorporation of dapoxetine-tetraphenyl borate (DAP–TPB) as a sensing element into a commercial nail varnish containing cellulose acetate propionate. The composite was applied onto a 3 mm diameter graphite disk electrode. The electrode exhibited a Nernstian slope of 56.0 mV/decade in the concentration range of 1 × 10−4 to 1 × 10−2 mol/L with an LOD of 2 × 10−5 mol/L. The electrode is independent of pH in the range of 2 to 6 and showed good selectivity for DAP with respect to a large number of inorganic cations and amino acids. Comparable Nernstian slope, sensitivity, pH range, and selectivity pattern were obtained with a PVC membrane and a carbon paste incorporating DAP–TPB as a sensing element and dioctylphthalate as a solvent mediator. The electrodes were used for the determination of DAP in pure solution and in tablets without extraction with high accuracy and precision (RSD ≤ 2%). The nail varnish solid-state electrode is simple, economical, and rapid when compared with PVC membrane and carbon paste electrodes.
Determination of Fructans in Infant, Adult, and Pediatric Nutritional Formulas: Single-Laboratory Validation, First Action 2016.06
Authors: Haselberger Philip , Jacobs Wesley A 
Published: September 2016

Abstract
A method for fructan analysis designed to comply with AOAC Standard Method Performance Requirements (SMPR®) 2014.002 is described. It is closely related to existing methods for fructan analysis, including AOAC 997.08 and 999.03, as well as a method previously published by Cuany et al. This new method achieves LOQ of 0.03% fructan on a ready-to-feed (RTF) basis with mean recoveries ranging from 93 to 108% in the presence of up to 9% sucrose (even at the 0.03% level of fructan). Repeatability ranged from 1.09 to 3.67%. Intermediate precision ranged from 2.46 to 6.79%. Sample preparation for quantitative analysis is simplified compared to some of the existing methodologies. The method incorporates a qualitative profile analysis to determine fructan size category. This allows assignment of appropriate correction factors without independent knowledge of fructan type.
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes
Authors: Lin Yongshun , Linask Kaari L. , Mallon Barbara , Johnson Kory , Klein Michael , Beers Jeanette , Xie Wen , Du Yubin , Liu Chengyu , Lai Yinzhi , Zou Jizhong , Haigney Mark , Yang Hushan , Rao Mahendra , Chen Guokai 
Published: September 2016

AbstractCardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment with heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined Dulbecco's modified Eagle's medium/F-12-based medium on either Matrigel or defined matrices like vitronectin and Synthemax. One of heparin's main functions was to act as a Wnt modulator that helped promote robust and consistent cardiomyocyte production. Our study provides an efficient, reliable, and cost-effective method for cardiomyocyte derivation from hPSCs that can be used for potential large-scale drug screening, disease modeling, and future cellular therapies.