bluuebunny/crossref_metadata_2025_split huggingface dataset:
Haken spheres for genus two Heegaard splittings
Authors: CHO SANGBUM , KODA YUYA 
Published: October 2017

AbstractA manifold which admits a reducible genus-2 Heegaard splitting is one of the 3-sphere, S2 × S1, lens spaces or their connected sums. For each of those splittings, the complex of Haken spheres is defined. When the manifold is the 3-sphere, S2 × S1 or a connected sum whose summands are lens spaces or S2 × S1, the combinatorial structure of the complex has been studied by several authors. In particular, it was shown that those complexes are all contractible. In this work, we study the remaining cases, that is, when the manifolds are lens spaces. We give a precise description of each of the complexes for the genus-2 Heegaard splittings of lens spaces. A remarkable fact is that the complexes for most lens spaces are not contractible and even not connected.
Building a Modern City: Legacies of Residential Development and Architectural Adaptation in Colonial Hong Kong
Authors: LAU LEUNG KWOK PRUDENCE 
Published: October 2017

AbstractThis article focuses on the relationship between property developers and architects in colonial Hong Kong in the 1920s to 1930s, identifying a successful collaboration within the architectural development company, the Crédit Foncier d'Extrême-Orient (CFEO). Benefiting from new plots of land made available on the Kowloon Peninsula and the opportunity to plan new neighbourhoods for both Western and Chinese clientele, the directors of CFEO negotiated with their in-house architects regarding land speculation and residential typology preferences while targeting the middle-class market. The company's demolished buildings left a crucial gap in the urban history of Hong Kong and China's treaty ports only recently uncovered through archival research by the author. Drawing upon in-depth primary data research and interviews with the architects’ descendants, this article probes into early twentieth-century residential development in Hong Kong, revealing how certain Western entrepreneurs and architects experimented with unique adaptations of architectural typologies suited to the local environment in the Chinese urban landscape.
Circulation: reflections on circularity, entity, and liquidity in the language of global history
Authors: Gänger Stefanie 
Published: October 2017

Abstract‘Circulation’ is not only among the most widely used words in the language of global history; it is also among the most erratically employed. Amorphous in its usages and protean in its semantics, ‘circulation’ has come to describe any sort of movement: from circular movement and passage along the vessels of closed systems to, paradoxically, open-ended, unidirectional dissemination. This article asks how ‘circulation’ became prominent metaphorically in global history; it seeks to understand the word’s appeal and the consequences of its ascendancy. It argues that the popularity of ‘circulation’ is attributable to a merger of two of its qualities: its seeming ‘untainted-ness’ and openness, on the one hand, and on the other, how its older, medical and economic, meanings resonate in its usages, allowing it to convey a sense of entity (independent existence) for the terrain in which ‘circulation’ occurs, and a sense of directedness, self-reliance, and ‘liquidity’ for the movements it describes.
Coping and Mindfulness
Authors: Ataşalar Jale , Michou Aikaterini 
Published: October 2017

Abstract. Problematic Internet use (PIU) has been posited as the negative outcome of unmet psychological needs in real life. The present study, relying on the cognitive-behavioral model of PIU ( Brand, Young, & Laier, 2014 ; Davis, 2001 ) and self-determination theory ( Deci & Ryan, 2000 ), investigated the extent to which coping strategies in aversive situations and mindfulness during Internet use serve as mediating mechanisms in the relation between need satisfaction and generalized PIU (GPIU; dependency on multiple functions of the Internet). Path analysis on a sample of 165 Turkish early adolescents ( Mage = 12.88, SD = .83; 49.1% females) found that need satisfaction was negatively related to PIU via low avoidant coping and high mindfulness in Internet engagement. The findings support the pathways from disadvantageous social context to GPIU suggested by Brand and colleagues’ (2014) model of GPIU and additionally show that next to avoidant coping, online mindfulness, an indicator of loss of cognitive control, can be a proximal correlate of GPIU. Interventions for adolescents’ harmonious Internet use could focus, among others, on adolescents’ need satisfaction, awareness of coping strategies, and development of online mindfulness.
Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells
Authors: Orecchioni Marco , Bedognetti Davide , Newman Leon , Fuoco Claudia , Spada Filomena , Hendrickx Wouter , Marincola Francesco M. , Sgarrella Francesco , Rodrigues Artur Filipe , Ménard-Moyon Cécilia , Cesareni Gianni , Kostarelos Kostas , Bianco Alberto , Delogu Lucia G 
Published: October 2017

AbstractUnderstanding the biomolecular interactions between graphene and human immune cells is a prerequisite for its utilization as a diagnostic or therapeutic tool. To characterize the complex interactions between graphene and immune cells, we propose an integrative analytical pipeline encompassing the evaluation of molecular and cellular parameters. Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO) and GO functionalized with amino groups (GONH2) on 15 immune cell populations, interrogating 30 markers at the single-cell level. Next, the integration of single-cell mass cytometry with genome-wide transcriptome analysis shows that the amine groups reduce the perturbations caused by GO on cell metabolism and increase biocompatibility. Moreover, GONH2 polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response. This study describes an innovative approach for the analysis of the effects of nanomaterials on distinct immune cells, laying the foundation for the incorporation of single-cell mass cytometry on the experimental pipeline.
Chiral transcription in self-assembled tetrahedral Eu4L6 chiral cages displaying sizable circularly polarized luminescence
Authors: Yeung Chi-Tung , Yim King-Him , Wong Ho-Yin , Pal Robert , Lo Wai-Sum , Yan Siu-Cheong , Yee-Man Wong Melody , Yufit Dmitry , Smiles Danil E. , McCormick Laura J. , Teat Simon J. , Shuh David K. , Wong Wing-Tak , Law Ga-Lai 
Published: October 2017

AbstractPredictable stereoselective formation of supramolecular assembly is generally believed to be an important but complicated process. Here, we show that point chirality of a ligand decisively influences its supramolecular assembly behavior. We designed three closely related chiral ligands with different point chiralities, and observe their self-assembly into europium (Eu) tetrametallic tetrahedral cages. One ligand exhibits a highly diastereoselective assembly into homochiral (either ΔΔΔΔ or ΛΛΛΛ) Eu tetrahedral cages whereas the two other ligands, with two different approaches of loosened point chirality, lead to a significant breakdown of the diastereoselectivity to generate a mixture of (ΔΔΔΔ and ΛΛΛΛ) isomers. The cages are highly emissive (luminescence quantum yields of 16(1) to 18(1)%) and exhibit impressive circularly polarized luminescence properties (|glum|: up to 0.16). With in-depth studies, we present an example that correlates the nonlinear enhancement of the chiroptical response to the nonlinearity dependence on point chirality.
αv integrins on mesenchymal cells regulate skeletal and cardiac muscle fibrosis
Authors: Murray I. R. , Gonzalez Z. N. , Baily J. , Dobie R. , Wallace R. J. , Mackinnon A. C. , Smith J. R. , Greenhalgh S. N. , Thompson A. I. , Conroy K. P. , Griggs D. W. , Ruminski P. G. , Gray G. A. , Singh M. , Campbell M. A. , Kendall T. J. , Dai J. , Li Y. , Iredale J. P. , Simpson H. , Huard J. , Péault B. , Henderson N. C. 
Published: October 2017

AbstractMesenchymal cells expressing platelet-derived growth factor receptor beta (PDGFRβ) are known to be important in fibrosis of organs such as the liver and kidney. Here we show that PDGFRβ+ cells contribute to skeletal muscle and cardiac fibrosis via a mechanism that depends on αv integrins. Mice in which αv integrin is depleted in PDGFRβ+ cells are protected from cardiotoxin and laceration-induced skeletal muscle fibrosis and angiotensin II-induced cardiac fibrosis. In addition, a small-molecule inhibitor of αv integrins attenuates fibrosis, even when pre-established, in both skeletal and cardiac muscle, and improves skeletal muscle function. αv integrin blockade also reduces TGFβ activation in primary human skeletal muscle and cardiac PDGFRβ+ cells, suggesting that αv integrin inhibitors may be effective for the treatment and prevention of a broad range of muscle fibroses.
Functional reconstruction of a eukaryotic-like E1/E2/(RING) E3 ubiquitylation cascade from an uncultured archaeon
Authors: Hennell James Rory , Caceres Eva F. , Escasinas Alex , Alhasan Haya , Howard Julie A. , Deery Michael J. , Ettema Thijs J. G. , Robinson Nicholas P. 
Published: October 2017

AbstractThe covalent modification of protein substrates by ubiquitin regulates a diverse range of critical biological functions. Although it has been established that ubiquitin-like modifiers evolved from prokaryotic sulphur transfer proteins it is less clear how complex eukaryotic ubiquitylation system arose and diversified from these prokaryotic antecedents. The discovery of ubiquitin, E1-like, E2-like and small-RING finger (srfp) protein components in the Aigarchaeota and the Asgard archaea superphyla has provided a substantive step toward addressing this evolutionary question. Encoded in operons, these components are likely representative of the progenitor apparatus that founded the modern eukaryotic ubiquitin modification systems. Here we report that these proteins from the archaeon Candidatus ‘Caldiarchaeum subterraneum’ operate together as a bona fide ubiquitin modification system, mediating a sequential ubiquitylation cascade reminiscent of the eukaryotic process. Our observations support the hypothesis that complex eukaryotic ubiquitylation signalling pathways have developed from compact systems originally inherited from an archaeal ancestor.
DNA N6-methyladenine is dynamically regulated in the mouse brain following environmental stress
Authors: Yao Bing , Cheng Ying , Wang Zhiqin , Li Yujing , Chen Li , Huang Luoxiu , Zhang Wenxin , Chen Dahua , Wu Hao , Tang Beisha , Jin Peng 
Published: October 2017

AbstractChemical modifications on DNA molecules, such as 5-methylcytosine and 5-hydroxymethylcytosine, play important roles in the mammalian brain. A novel DNA adenine modification, N(6)-methyladenine (6mA), has recently been found in mammalian cells. However, the presence and function(s) of 6mA in the mammalian brain remain unclear. Here we demonstrate 6mA dynamics in the mouse brain in response to environmental stress. We find that overall 6mA levels are significantly elevated upon stress. Genome-wide 6mA and transcriptome profiling reveal an inverse association between 6mA dynamic changes and a set of upregulated neuronal genes or downregulated LINE transposon expression. Genes bearing stress-induced 6mA changes significantly overlap with loci associated with neuropsychiatric disorders. These results suggest an epigenetic role for 6mA in the mammalian brain as well as its potential involvement in neuropsychiatric disorders.
Recycled iron fuels new production in the eastern equatorial Pacific Ocean
Authors: Rafter Patrick A. , Sigman Daniel M. , Mackey Katherine R. M. 
Published: October 2017

AbstractNitrate persists in eastern equatorial Pacific surface waters because phytoplankton growth fueled by nitrate (new production) is limited by iron. Nitrate isotope measurements provide a new constraint on the controls of surface nitrate concentration in this region and allow us to quantify the degree and temporal variability of nitrate consumption. Here we show that nitrate consumption in these waters cannot be fueled solely by the external supply of iron to these waters, which occurs by upwelling and dust deposition. Rather, a substantial fraction of nitrate consumption must be supported by the recycling of iron within surface waters. Given plausible iron recycling rates, seasonal variability in nitrate concentration on and off the equator can be explained by upwelling rate, with slower upwelling allowing for more cycles of iron regeneration and uptake. The efficiency of iron recycling in the equatorial Pacific implies the evolution of ecosystem-level mechanisms for retaining iron in surface ocean settings where it limits productivity.
Fine-tuning of auxin homeostasis governs the transition from floral stem cell maintenance to gynoecium formation
Authors: Yamaguchi Nobutoshi , Huang Jiangbo , Xu Yifeng , Tanoi Keitaro , Ito Toshiro 
Published: October 2017

AbstractTo ensure successful plant reproduction and crop production, the spatial and temporal control of the termination of the floral meristem must be coordinated. In Arabidopsis, the timing of this termination is determined by AGAMOUS (AG). Following its termination, the floral meristem underdoes gynoecium formation. A direct target of AG, CRABS CLAW (CRC), is involved in both floral meristem determinacy and gynoecium development. However, how floral meristem termination is coordinated with gynoecium formation is not understood. Here, we identify a mechanistic link between floral meristem termination and gynoecium development through fine-tuning of auxin homeostasis by CRC. CRC controls auxin homeostasis in the medial region of the developing gynoecium to generate proper auxin maxima. This regulation partially occurs via direct transcriptional repression of TORNADO2 (TRN2) by CRC. Plasma membrane-localized TRN2 modulates auxin homeostasis. We propose a model describing how regulation of auxin homeostasis mediates the transition from floral meristem termination to gynoecium development.
Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs
Authors: Lyons Shawn M. , Gudanis Dorota , Coyne Steven M. , Gdaniec Zofia , Ivanov Pavel 
Published: October 2017

AbstractRNA G-quadruplex (RG4) structures are involved in multiple biological processes. Recent genome-wide analyses of human mRNA transcriptome identified thousands of putative intramolecular RG4s that readily assemble in vitro but shown to be unfolded in vivo. Previously, we have shown that mature cytoplasmic tRNAs are cleaved during stress response to produce tRNA fragments that function to repress translation in vivo. Here we report that these bioactive tRNA fragments assemble into intermolecular RG4s. We provide evidence for the formation of uniquely stable tetramolecular RG4 structures consisting of five tetrad layers formed by 5ʹ-terminal oligoguanine motifs of an individual tRNA fragment. RG4 is required for functions of tRNA fragments in the regulation of mRNA translation, a critical component of cellular stress response. RG4 disruption abrogates tRNA fragments ability to trigger the formation of Stress Granules in vivo. Collectively, our data rationalize the existence of naturally occurring RG4-assembling tRNA fragments and emphasize their regulatory roles.
Elevated CO2 degassing rates prevented the return of Snowball Earth during the Phanerozoic
Authors: Mills Benjamin J. W. , Scotese Christopher R. , Walding Nicholas G. , Shields Graham A. , Lenton Timothy M. 
Published: October 2017

AbstractThe Cryogenian period (~720–635 Ma) is marked by extensive Snowball Earth glaciations. These have previously been linked to CO2 draw-down, but the severe cold climates of the Cryogenian have never been replicated during the Phanerozoic despite similar, and sometimes more dramatic changes to carbon sinks. Here we quantify the total CO2 input rate, both by measuring the global length of subduction zones in plate tectonic reconstructions, and by sea-level inversion. Our results indicate that degassing rates were anomalously low during the Late Neoproterozoic, roughly doubled by the Early Phanerozoic, and remained comparatively high until the Cenozoic. Our carbon cycle modelling identifies the Cryogenian as a unique period during which low surface temperature was more easily achieved, and shows that the shift towards greater CO2 input rates after the Cryogenian helped prevent severe glaciation during the Phanerozoic. Such a shift appears essential for the development of complex animal life.
The making of a miscreant: tobacco smoke and the creation of pathogen-rich biofilms
Authors: Shah Samir A. , Ganesan Sukirth M. , Varadharaj Saradhadevi , Dabdoub Shareef M. , Walters John D. , Kumar Purnima S. 
Published: October 2017

AbstractWe have previously reported that oral biofilms in clinically healthy smokers are pathogen-rich, and that this enrichment occurs within 24 h of biofilm formation. The present investigation aimed to identify a mechanism by which smoking creates this altered community structure. By combining in vitro microbial–mucosal interface models of commensal (consisting of Streptococcus oralis, Streptococcus sanguis, Streptococcus mitis, Actinomyces naeslundii, Neisseria mucosa and Veillonella parvula) and pathogen-rich (comprising S.oralis, S.sanguis, S.mitis, A.naeslundii, N.mucosa and V.parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, Filifactor alocis, Dialister pneumosintes, Selenonomas sputigena, Selenominas noxia, Catonella morbi, Parvimonas micra and Tannerella forsythia) communities with metatranscriptomics, targeted proteomics and fluorescent microscopy, we demonstrate that smoke exposure significantly downregulates essential metabolic functions within commensal biofilms, while significantly increasing expression of virulence genes, notably lipopolysaccharide (LPS), flagella and capsule synthesis. By contrast, in pathogen-rich biofilms several metabolic pathways were over-expressed in response to smoke exposure. Under smoke-rich conditions, epithelial cells mounted an early and amplified pro-inflammatory and oxidative stress response to these virulence-enhanced commensal biofilms, and a muted early response to pathogen-rich biofilms. Commensal biofilms also demonstrated early and widespread cell death. Similar results were observed when smoke-free epithelial cells were challenged with smoke-conditioned biofilms, but not vice versa. In conclusion, our data suggest that smoke-induced transcriptional shifts in commensal biofilms triggers a florid pro-inflammatory response, leading to early commensal death, which may preclude niche saturation by these beneficial organisms. The cytokine-rich, pro-oxidant, anaerobic environment sustains inflammophilic bacteria, and, in the absence of commensal antagonism, may promote the creation of pathogen-rich biofilms in smokers.
Nematic topological superconducting phase in Nb-doped Bi2Se3
Authors: Shen Junying , He Wen-Yu , Yuan Noah Fan Qi , Huang Zengle , Cho Chang-woo , Lee Seng Huat , Hor Yew San , Law Kam Tuen , Lortz Rolf 
Published: October 2017

AbstractA nematic topological superconductor has an order parameter symmetry, which spontaneously breaks the crystalline symmetry in its superconducting state. This state can be observed, for example, by thermodynamic or upper critical field experiments in which a magnetic field is rotated with respect to the crystalline axes. The corresponding physical quantity then directly reflects the symmetry of the order parameter. We present a study on the superconducting upper critical field of the Nb-doped topological insulator NbxBi2Se3 for various magnetic field orientations parallel and perpendicular to the basal plane of the Bi2Se3 layers. The data were obtained by two complementary experimental techniques, magnetoresistance and DC magnetization, on three different single crystalline samples of the same batch. Both methods and all samples show with perfect agreement that the in-plane upper critical fields clearly demonstrate a two-fold symmetry that breaks the three-fold crystal symmetry. The two-fold symmetry is also found in the absolute value of the magnetization of the initial zero-field-cooled branch of the hysteresis loop and in the value of the thermodynamic contribution above the irreversibility field, but also in the irreversible properties such as the value of the characteristic irreversibility field and in the width of the hysteresis loop. This provides strong experimental evidence that Nb-doped Bi2Se3 is a nematic topological superconductor similar to the Cu- and Sr-doped Bi2Se3.
Deconstructing the traditional Japanese medicine “Kampo”: compounds, metabolites and pharmacological profile of maoto, a remedy for flu-like symptoms
Authors: Nishi Akinori , Ohbuchi Katsuya , Kushida Hirotaka , Matsumoto Takashi , Lee Keiko , Kuroki Haruo , Nabeshima Shigeki , Shimobori Chika , Komokata Nagisa , Kanno Hitomi , Tsuchiya Naoko , Zushi Makoto , Hattori Tomohisa , Yamamoto Masahiro , Kase Yoshio , Matsuoka Yukiko , Kitano Hiroaki 
Published: October 2017

AbstractPharmacological activities of the traditional Japanese herbal medicine (Kampo) are putatively mediated by complex interactions between multiple herbal compounds and host factors, which are difficult to characterize via the reductive approach of purifying major bioactive compounds and elucidating their mechanisms by conventional pharmacology. Here, we performed comprehensive compound, pharmacological and metabolomic analyses of maoto, a pharmaceutical-grade Kampo prescribed for flu-like symptoms, in normal and polyI:C-injected rats, the latter suffering from acute inflammation via Toll-like receptor 3 activation. In total, 352 chemical composition-determined compounds (CCDs) were detected in maoto extract by mass spectrometric analysis. After maoto treatment, 113 CCDs were newly detected in rat plasma. Of these CCDs, 19 were present in maoto extract, while 94 were presumed to be metabolites generated from maoto compounds or endogenous substances such as phospholipids. At the phenotypic level, maoto ameliorated the polyI:C-induced decrease in locomotor activity and body weight; however, body weight was not affected by individual maoto components in isolation. In accordance with symptom relief, maoto suppressed TNF-α and IL-1β, increased IL-10, and altered endogenous metabolites related to sympathetic activation and energy expenditure. Furthermore, maoto decreased inflammatory prostaglandins and leukotrienes, and increased anti-inflammatory eicosapentaenoic acid and hydroxyl-eicosapentaenoic acids, suggesting that it has differential effects on eicosanoid metabolic pathways involving cyclooxygenases, lipoxygenases and cytochrome P450s. Collectively, these data indicate that extensive profiling of compounds, metabolites and pharmacological phenotypes is essential for elucidating the mechanisms of herbal medicines, whose vast array of constituents induce a wide range of changes in xenobiotic and endogenous metabolism.