bluuebunny/crossref_metadata_2025_split huggingface dataset:
ORBIT: a new paradigm for genetic engineering of mycobacterial chromosomes
Authors: Murphy Kenan C. , Nelson Samantha J. , Nambi Subhalaxmi , Papavinasasundaram Kadamba , Baer Christina E. , Sassetti Christopher M. 
Published: January 2018

ABSTRACTCurrent methods for genome engineering in mycobacteria rely on relatively inefficient recombination systems that require the laborious construction of a long double-stranded DNA substrate for each desired modification. We combined two efficient recombination systems to produce a versatile method for high-throughput chromosomal engineering that obviates the need for the preparation of double-stranded DNA recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous recombination mediated by the phage Che9c RecT annelase. This oligo contains a site-specific recombination site for the directional Bxb1 integrase (Int), which allows the simultaneous integration of a “payload plasmid” that contains a cognate recombination site and selectable marker. The targeting oligo and payload plasmid are co-transformed into a RecT‐ and Int-expressing strain, and drug-resistant homologous recombinants are selected in a single step. A library of reusable target-independent payload plasmids is available to generate knockouts and promoter replacements, or to fuse the C-terminal-encoding regions of target genes with tags of various functionalities. This new system is called ORBIT (Oligo-mediated Recombineering followed by Bxb1 Integrase Targeting) and is ideally suited for the creation of libraries consisting of large numbers of deletions, insertions or fusions in a target bacterium. We demonstrate the utility of ORBIT by the construction of insertions or deletions in over 100 genes inM. tuberculosisandM. smegmatis. The report describes the first genetic engineering technique for making selectable chromosomal fusions and deletions that does not require the construction of target‐ or modification-specific double-stranded DNA recombination substrates.
Buffering relationship conflict consequences in teams working in real organizations
Authors: Benitez Miriam , Medina Francisco J. , Munduate Lourdes 
Published: January 2018

PurposeRelationship conflict has important negative organizational and personal consequences. However, papers analyzing how to buffer the negative effects of relationship conflict at work-unit level are lacking. This study aims to extend the literature by examining which specific conflict management styles used by work teams (avoiding, integrating and compromising) reduce or increase the link between relationship conflict and collective emotional exhaustion.Design/methodology/approachRegression analysis was conducted using 91 teams (398 employees) from 42 hotels and 42 restaurants.FindingsResults revealed that, as it was expected, relationship conflict was positively related to emotional exhaustion at a team level; this relationship depended on how team members handle relationship conflicts. That is, avoiding and integrating conflict management styles buffered the link between relationship conflict and collective emotional exhaustion, whereas compromising increased this positive link.Research limitations/implicationsOrganizations would include conflict management skills as a requirement for preventing negative consequences of conflict in teams, such as anxiety/depression and bullying.Originality/valueBy considering the unique perspective of team member’s shared perceptions of conflict management styles, important implications for the span of influence of collective perception of conflict on well-being have been indicated.
Transnational higher education
Authors: Heffernan Troy , Wilkins Stephen , Butt Muhammad Mohsin 
Published: January 2018

PurposeThe purpose of this paper is to assess the extent to which the critical relational variables of university reputation, student trust and student-university identification influence student behaviour towards transnational education partnerships.Design/methodology/approachStudents undertaking British degrees at two transnational partnership locations (Hong Kong,n=203 and Sri Lanka,n=325) completed a quantitative survey questionnaire. A conceptual model was developed and tested using structural equation modelling.FindingsUniversity reputation and student trust were found to be significant predictors of student identification with each partner institution, and student-university identification was a significant predictor of student satisfaction, loyalty and extra-role behaviours towards both the local and foreign educational organisations.Practical implicationsThe findings suggest that student relationship management strategies should focus on strengthening the higher education institution’s reputation, and increasing the students’ trust and identification with the institution. Moreover, universities should also assess potential partners for these qualities when entering into transnational education partnerships.Originality/valueDrawing on theories of social and organisational identification, this is the first study to consider student-university identification as the linchpin between the exogenous constructs of reputation and trust, and the endogenous constructs of student satisfaction, loyalty and extra-role behaviours in both the international education and international business literatures.
Entrepreneurial behaviour formation among Farmers
Authors: Yaseen Asif , Somogyi Simon , Bryceson Kim 
Published: January 2018

PurposeThe purpose of this paper is to investigate how farmers perceive and exploit business opportunities to foster entrepreneurship in developing country agriculture.Design/methodology/approachIn total, 174 milk producers completed a face-to-face survey within a posttest- pretest research design. Partial least squares structural equation modelling (PLS-SEM) was used to test the hypotheses.FindingsResults revealed that intentions, channelled through desirability, feasibility and optimism, become a strong predictor to recognise the opportunity to be entrepreneurial; however, the presence of a munificent environment and participation in apprenticeship and training programmes are the main and direct source of exploiting farming business opportunities.Research limitations/implicationsThe major limitation of the study is that cross-sectional data collected only from milk producers in Pakistan, signifying a need to include other agricultural sectors across different developing countries for further contextualising the results.Originality/valueResearch on entrepreneurial behaviour among farmers is scant. This study emphasises how cognitive heuristics guide intentions influencing the process of opportunity formation, and a munificent environment and entrepreneurial skills trainings are necessary for starting dairy farming business with modern practices.
Do seasonality, break and spillover effects explain commodity price volatility
Authors: Maitra Debashish 
Published: January 2018

PurposeThe purpose of this paper is to understand the volatility in commodity futures and spot markets. The study starts with a few questions: first, the effect of seasonality on the volatility is studied. Thereafter, the presence of structural breaks in the variance is identified. At last the seasonality, structural shifts and spillover effects are examined together to find out their effects on volatility.Design/methodology/approachThe methodology heavily employs econometric tools and techniques. The monthly seasonal dummies are incorporated to identify the effects of seasonality on volatility. Then, the presence of break in volatility is tested by cumulative sum of squares (CUSUM test), followed by generalized autoregressive conditional heteroscedastictity and EGARCH models are measured by including seasonal dummies, break dummies and the residuals of other market in the variance equation to determine spillover effects.FindingsIt is found that the effects of seasonality on volatility cannot be ignored as the effects are significant. The presence of asymmetry is detected in all the commodities. The presence of seasonality and structural breaks in the variance equation are statistically able to reduce the volatility but the magnitude is very negligible with an exception in cumin futures markets. Bi-directional volatility spillover between futures and spot markets is observed in all the commodities and the effect of spillover is more from spot markets to the futures markets.Research limitations/implicationsThis study is limited to a few agro commodities which are well traded. This study could have been extended to the other thinly traded commodities. This study has also taken only near month futures contracts as it contains more information but the same could have been studied by taking far month contracts also.Originality/valueThe present study attempted to understand the conjugated effects of seasonality, structural breaks and spillover on volatility of commodity markets which is not apparent in the previous studies. This study has also employed methodological rigor to identify the breaks in the variance equation. In addition to this it has also investigated whether Indian commodity futures markets are informationally more efficient than the spot markets.
Multi-stakeholder partnerships in value chain development
Authors: Mutebi Kalibwani Rebecca , Twebaze Jennifer , Kamugisha Rick , Kakuru Medard , Sabiiti Moses , Kugonza Irene , Tenywa Moses , Nyamwaro Sospeter 
Published: January 2018

Purpose
The purpose of this paper is to demonstrate that agricultural commodity value chain development using multi-stakeholder partnerships (MSPs) can fast-track improvement in the livelihoods of rural farming households. With the view that such partnerships can raise farmers’ incomes, the study uses the case of the organic pineapple (OP) value chain in Ntungamo, Western Uganda, to understand the governance features that hold the value chain partners together, to analyse the costs and margins to the participating farmers, to identify opportunities for demand-driven upgrading of the farmers’ skills and knowledge, and the role that partnerships play in such upgrading.

Design/methodology/approach
The study uses the qualitative tools of value chain analysis: value chain maps of stakeholders, processes and support services of the OP value chain, and a quantitative tool to analyse costs and margins to the participating farmers. Interviews were conducted with key informants from the OP innovation platform, and survey data collected for the planting season, February–July, 2014, across three farmer categories of certified organic, conventional, and farmers not participating in the innovation platform.

Findings
Careful selection of partnerships to develop the value chain is found to be critical. Partners to involve should be those that enable the upgrading of farmers’ knowledge, skills and technologies to position them for better markets. Partners should also include those that enable the improvement of margins to the farmers and efficiency of the value chain. The strategic MSPs should be bound by formal contracts, to ensure stable relationships in the value chain and hence sustainable market access for the farmers.

Research limitations/implications
Although carried out on a specific value chain in a specific local context, this is not likely to limit the applicability of the findings to commodity value chains in a range of local contexts.

Originality/value
The study fulfils the need to highlight the role that stakeholder partnerships can play in value chain development and how they can be sustained by governance and institutional arrangements.
Acting and performing: influences of manager emotional intelligence
Authors: Prati L. Melita , Karriker Joy H. 
Published: January 2018

PurposeThe purpose of this paper is to examine the predictive role of manager emotional intelligence (MEI) with regard to emotional labor efforts and outcomes relationships. Testing evaluated the influence of MEI on the effects of emotional labor activities employed by subordinates (e.g. surface and active deep acting).Design/methodology/approachRelationships were examined between the emotional labor efforts employed by subordinates and their levels of depressed mood, as well as the supervisory leaders’ evaluations of the subordinates’ emotional performance resulting indirectly and directly from the emotional labor activities. Specifically, hypotheses that MEI will influence the performance of emotional labor and the effects of such activities on depressed mood and emotional performance were tested.FindingsFindings indicate that MEI moderates the impact of depressed mood on emotional performance.Originality/valueThis research adds to the emotional intelligence literature and enhances practical understanding of how organizations dependent on relationship-oriented, successful customer care can sponsor employee actions that facilitate results.
The effect of employee ability, hospital’s ethic and leadership on job satisfaction through employee commitment
Authors: Mardiana Yusuf Ria 
Published: January 2018

PurposeThe purpose of this paper is to confirm the direct and indirect effect of employee ability, perceived ethic, and leadership and leadership to service quality through employee commitment.Design/methodology/approachA survey instrument is using to collect the data and to measure the effect of employee ability, perceived ethic, and leadership directly to the quality of inpatient health care services was developed. This study also used the self-perception, leadership evaluation and customer evaluation questionnaires. The effects among variables were also analysed by using the structural equation modelling method.FindingsThe results of the analysis show that the survey instrument was reliable and valid; the implementation of hospital ethic was more emphasised on the nurses (as a front liners); the study proved that there is a direct and indirect effect among variables of employee ability, hospital’s ethic, and leadership to the service quality through the employee commitment; the direct effect of employee commitment variables against the service quality was significantly negative.Research limitations/implicationsAnalyses are developed within the context of a government single hospital of Type A. According to the time sequence of study, this field of study research considered for purpose only (one time study), and was not conducted for experimental study.Originality/valueThis paper’s main contribution is to propose and empirically test a set of constructs that complement the effect of employee ability, hospital’s perceived ethic, leadership on the service quality of inpatient health care through the employee commitment direct and indirectly in the Type A hospital.
A case report and literature review of autism and attention deficit hyperactivity disorder in paediatric chronic pain
Authors: Wiwe Lipsker Camilla , von Heijne Margareta , Bölte Sven , Wicksell Rikard K. 
Published: January 2018

AbstractPsychiatric disorders are common in paediatric patients with chronic pain, but the overall prevalence of comorbid neurodevelopmental disorders is unclear. We report on a case of severe chronic pain in a child with undiagnosed comorbid autism spectrum disorder and attention deficit hyperactivity disorder, where significant improvements in pain and function occurred following methylphenidate medication and parental behavioural training.ConclusionThe inclusion of behavioural assessment and screening for neurodevelopmental comorbidity may be essential in addressing complex paediatric chronic pain.
Autonomous Purkinje cell axonal dystrophy causes ataxia in peroxisomal multifunctional protein‐2 deficiency
Authors: De Munter Stephanie , Bamps Dorien , Malheiro Ana Rita , Kumar Baboota Ritesh , Brites Pedro , Baes Myriam 
Published: January 2018

AbstractPeroxisomes play a crucial role in normal neurodevelopment and in the maintenance of the adult brain. This depends largely on intact peroxisomal β‐oxidation given the similarities in pathologies between peroxisome biogenesis disorders and deficiency of multifunctional protein‐2 (MFP2), the central enzyme of this pathway. Recently, adult patients diagnosed with cerebellar ataxia were shown to have mild mutations in the MFP2 gene, hydroxy‐steroid dehydrogenase (17 beta) type 4 (HSD17B4). Cerebellar atrophy also develops in MFP2 deficient mice but the cellular origin of the degeneration is unexplored. In order to investigate whether peroxisomal β‐oxidation is essential within Purkinje cells, the sole output neurons of the cerebellum, we generated and characterized a mouse model with Purkinje cell selective deletion of the MFP2 gene. We show that selective loss of MFP2 from mature cerebellar Purkinje neurons causes a late‐onset motor phenotype and progressive Purkinje cell degeneration, thereby mimicking ataxia and cerebellar deterioration in patients with mild HSD17B4 mutations. We demonstrate that swellings on Purkinje cell axons coincide with ataxic behavior and precede neurodegeneration. Loss of Purkinje cells occurs in a characteristic banded pattern, proceeds in an anterior to posterior fashion and is accompanied by progressive astro‐ and microgliosis. These data prove that the peroxisomal β‐oxidation pathway is required within Purkinje neurons to maintain their axonal integrity, independent of glial dysfunction.
Deferoxamine promotes mesenchymal stem cell homing in noise‐induced injured cochlea through PI3K/AKT pathway
Authors: Peyvandi A.A. , Abbaszadeh H.‐A. , Roozbahany N. Ahmady , Pourbakht A. , Khoshsirat S. , Niri H. Haddadzade , Peyvandi H. , Niknazar S. 
Published: January 2018

AbstractObjectiveOver 5% of the world's population suffers from disabling hearing loss. Stem cell homing in target tissue is an important aspect of cell‐based therapy, which its augmentation increases cell therapy efficiency. Deferoxamine (DFO) can induce the Akt activation, and phosphorylation status of AKT (p‐AKT) upregulates CXC chemokine receptor‐4 (CXCR4) expression. We examined whether DFO can enhance mesenchymal stem cells (MSCs) homing in noise‐induced damaged cochlea by PI3K/AKT dependent mechanism.Materials and MethodsMesenchymal stem cells were treated with DFO. AKT, p‐AKT protein and hypoxia inducible factor 1‐ α (HIF‐1α) and CXCR4 gene and protein expression was evaluated by RT‐ PCR and Western blot analysis. For in vivo assay, rats were assigned to control, sham, noise exposure groups without any treatment or receiving normal, DFO‐treated and DFO +LY294002 (The PI3K inhibitor)‐treated MSCs. Following chronic exposure to 115 dB white noise, MSCs were injected into the rat cochlea through the round window. Number of Hoechst‐ labelled cells was determined in the endolymph after 24 hours.ResultsDeferoxamine increased P‐AKT, HIF‐1α and CXCR4 expression in MSCs compared to non‐treated cells. DFO pre‐conditioning significantly increased the homing ability of MSCs into injured ear compared to normal MSCs. These effects of DFO were blocked by LY294002.ConclusionsPre‐conditioning of MSCs by DFO before transplantation can improve stem cell homing in the damaged cochlea through PI3K/AKT pathway activation.
Stiffness regulates the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells via the WNT signalling pathway
Authors: Liu Nanxin , Zhou Mi , Zhang Qi , Zhang Tao , Tian Taoran , Ma Quanquan , Xue Changyue , Lin Shiyu , Cai Xiaoxiao 
Published: January 2018

AbstractObjectivesResearches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness.Materials and methodsWe carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism.ResultsSoft substrates changed the cell morphology and inhibited the proliferation of DPSCs. Expression of markers related to osteogenic/odontogenic differentiation was significantly increased as the substrate stiffness increased, including ALP (alkaline phosphatase), OCN (osteocalcin), OPN (osteopontin), RUNX‐2 (runt‐related transcription factor‐2), BMP‐2 (bone morphogenetic protein‐2), DSPP (dentin sialophosphoprotein) and DMP‐1 (dentin matrix protein‐1). Mechanical properties promote the function of DPSCs related to the Wnt signalling pathway.ConclusionsOur results showed that mechanical factors can regulate the proliferation and differentiation of DPSCs via the WNT signalling pathway. This provides theoretical basis to optimize dental or bone tissue regeneration through increasing stiffness of extracelluar matrix.
The prescribable drugs with efficacy in experimental epilepsies (PDE3) database for drug repurposing research in epilepsy
Authors: Sivapalarajah Shayeeshan , Krishnakumar Mathangi , Bickerstaffe Harry , Chan YikYing , Clarkson Joseph , Hampden‐Martin Alistair , Mirza Ahmad , Tanti Matthew , Marson Anthony , Pirmohamed Munir , Mirza Nasir 
Published: January 2018

SummaryObjectiveCurrent antiepileptic drugs (AEDs) have several shortcomings. For example, they fail to control seizures in 30% of patients. Hence, there is a need to identify new AEDs. Drug repurposing is the discovery of new indications for approved drugs. This drug “recycling” offers the potential of significant savings in the time and cost of drug development. Many drugs licensed for other indications exhibit antiepileptic efficacy in animal models. Our aim was to create a database of “prescribable” drugs, approved for other conditions, with published evidence of efficacy in animal models of epilepsy, and to collate data that would assist in choosing the most promising candidates for drug repurposing.MethodsThe database was created by the following: (1) computational literature‐mining using novel software that identifies Medline abstracts containing the name of a prescribable drug, a rodent model of epilepsy, and a phrase indicating seizure reduction; then (2) crowdsourced manual curation of the identified abstracts.ResultsThe final database includes 173 drugs and 500 abstracts. It is made freely available at www.liverpool.ac.uk/D3RE/PDE3. The database is reliable: 94% of the included drugs have corroborative evidence of efficacy in animal models (for example, evidence from multiple independent studies). The database includes many drugs that are appealing candidates for repurposing, as they are widely accepted by prescribers and patients—the database includes half of the 20 most commonly prescribed drugs in England—and they target many proteins involved in epilepsy but not targeted by current AEDs. It is important to note that the drugs are of potential relevance to human epilepsy—the database is highly enriched with drugs that target proteins of known causal human epilepsy genes (Fisher's exact test P‐value < 3 × 10−5). We present data to help prioritize the most promising candidates for repurposing from the database.SignificanceThe PDE3 database is an important new resource for drug repurposing research in epilepsy.