bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design: An assay based on single-polypeptide-chain heterodimeric A2AR/D2R and non-oligomerized fusions forin vivoanalysis of their allosteric receptor-receptor interactions
Authors: Kamiya Toshio , Masuko Takashi , Borroto-Escuela Dasiel Oscar , Okado Haruo , Nakata Hiroyasu 
Published: July 2016

AbstractBackgroundThe adenosine A2Areceptor (A2AR) heteromerizes with the dopamine D2receptor (D2R). In order to explore their functional interaction, we engineered previously stable single-polypeptide-chain (sc) A2AR/D2LR: whether the molecular entity of the striatal A2AR/D2R antagonism, i.e., scA2AR/D2Rs are just A2AR/D2R with the antagonism, remains unresolved.New MethodTo further clarify the heteromerization through the scA2AR/D2LR, we here designed supramolecularly ‘exclusive’ monomers and dimers, using the Cε2 domain of IgE-Fc or apoproteins of the bacterial light-harvesting antenna complex.ResultsA concept of the recptor protein assembly regulation, i.e., the selective monomer/non-obligate dimer formation was obtained. Although none of these new fusions were constructed or tested, we could aim at obtaining heterodimer-specific agents, using the scA2AR/D2R. Whether the resulting designs were explained feasibly and rationally was addressed. The structure and function of the non-obligate dimer were here discussed through scA2AR/D2R, focusing on the procedure of the membrane protein design and methods for transient protein-protein interactions.Summary and OutlookGiven that upon being expressed and allosteric regulation occurs regardless of specific signal to non-specific noise (S/N) ratio, the supramolecular designs, allowing us to express selectively monomer/non-obligate dimer of class A GPCR, are experimentally testable and will be used to confirmin vivothat such low S/N ratio interaction between A2AR and D2LR functions in the dopamine neurotransmission in the striatum.Abbreviations:A2ARadenosine A2Areceptor3HA-A2ARA2AR tagged with a triple HA epitopeBchlbacteriochlorophyllBRETbioluminescence resonance energy transferCcarboxy-terminalCDcluster of differentiationD2LR and D2SRthe long and short form of dopamine D2receptor, respectivelyFabantigen binding fragmentFcFc fragmentFcεRIhigh affinity receptor for IgEFRETfluorescence resonance energy transferG4San amino acid sequence consisting of a four-glycine-repeat followed by a serine residueGABAγ-aminobutyric acidGABABGABA type B receptorGPCRG protein-coupled receptorGttransducinHAhemagglutininHIVhuman immunodeficiency virusICintracellular loopsIgimmunoglobulinLHlight-harvesting antenna complexmAbmonoclonal antibodyMrmolecular weightNamino-terminalPDParkinson’s diseasePSphotosystemRCreaction centerRlucRenillaluciferasescsingle-chainTMtransmembrane3Dthree-dimensional
Comprehensive investigation of temporal and autism-associated cell type composition-dependent and independent gene expression changes in human brains
Authors: Yu Qianhui , He Zhisong 
Published: July 2016

AbstractThe functions of human brains highly depend on the precise temporal regulation of gene expression, and the temporal brain transcriptome profile across lifespan has been observed. The substantial transcriptome alteration in neural disorders like autism has also been observed and is thought to be important for the pathology. While the cell type composition is known to be variable in brains, it remains unclear how it contributes to the temporal and pathological transcriptome changes in brains. Here, we applied a transcriptome deconvolution procedure to an age series RNA-seq dataset of healthy and autism samples, to quantify the contribution of cell type composition in shaping the temporal and autism pathological transcriptome in human brains. We estimated that composition change was the primary factor of both types of transcriptome changes. On the other hand, genes with substantial composition-independent expression changes were also observed in both cases. Those temporal and autism pathological composition-independent changes, many of which are related to synaptic functions, indicate the important intracellular regulatory changes in human brains in both processes.
Emergent risks in business process change projects
Authors: Jurisch Marlen Christin , Rosenberg Zuzana , Krcmar Helmut 
Published: July 2016

Purpose– Even today still many business process change (BPC) initiatives fail and cause high overruns for organizations undergoing BPC initiatives. It is therefore important that BPC practitioners and researchers understand the risks inherent in BPC projects, and that they adapt their risk management processes to account for and mitigate these risks. Thus, the purpose of this paper is to investigate which emergent risks matter in BPC project.Design/methodology/approach– The authors adopted case survey methodology and investigated data from 130 case studies to show the nature and magnitude of relationships between organizational support risks, volatility risks, and BPC project and process performance.Findings– The results show that organizational support risks influence both the overall BPC project performance and process performance. Whereas, volatility risks influence project performance but appear to have no direct impact on the process performance. Both organizational support risks and volatility risks show influence on project management practices.Research limitations/implications– The study show several limitations that might be assigned to the case survey methodology, such as use of secondary data or publication bias.Practical implications– The authors provide considerable support which emergent risks matter in BPC projects.Originality/value– The contribution of this study takes several forms. It fills a gap in the literature concerning emergent risk factors inherent in BPC projects. The authors provided theoretical explanation of the effects of emergent risks on BPC project and process performance. And lastly, the authors have demonstrated the usefulness of case survey methodology in BPC research.
A framework to capture and reuse process knowledge in business process design and execution using social tagging
Authors: Rangiha Mohammad Ehson , Comuzzi Marco , Karakostas Bill 
Published: July 2016

Purpose
– The purpose of this paper is to present a framework for social business process management (BPM) in which social tagging is used to capture process knowledge emerging during the design and enactment of the processes. Process knowledge concerns both the type of activities chosen to fulfil a certain goal and the skills and experience of users in executing specific tasks. This knowledge is exploited by recommendation tools to support the design and enactment of current and future process instances.

Design/methodology/approach
– The literature about traditional BPM is analysed to highlight the limitations of traditional BPM regarding management of ad hoc and semi-structured processes. Having identified this gap, an innovative BPM framework based on social tagging is proposed to address these limitations. This model is exemplified in a real case scenario and evaluated through the implementation of a prototype and a case study in real world non-profit organisation.

Findings
– An overview of the social BPM framework is presented, introducing the concepts of role and task recommendation, which are supported by social tagging. The prototype shows the buildability of the social BPM framework as an extension of a Wiki platform. The case study demonstrates that the social BPM framework improves user collaborativeness in designing and executing process instances.

Research limitations/implications
– The applicability of the framework is targeted to ad hoc and possibly semi-structured business processes and it does not extend to highly procedural and codified processes. A single case study limits the generalisability of the evaluation results.

Originality/value
– The social BPM framework is the first to introduce task and role recommendation supported by social tagging to overcome the limitations of traditional BPM models.
Designing employees’ training by immersive serious games? A study of digital natives’ satisfaction, perception and expectation in corporate training practices
Authors: Lau Kung Wong , Lee Pui Yuen 
Published: July 2016

Purpose
The digital native (DN), a new type of employee, has reshaped the ways of corporate training. This DN wants meaningful play in game engagement instead of receiving a passive message from the employers. Meanwhile, the platforms of serious games had been changing from television to mobile, and currently moving to the immersive platforms. This paper aims to deepen the understanding of employees’ satisfaction, perception and expectation towards the use of serious games for training in business practices.

Design/methodology/approach
A quantitative survey with a 20-item questionnaire and a serious game have been used for collecting 57 DN’s opinions on the five significant features, and they are visualization, enjoyment, interactivity, immersion and communication.

Findings
The results found a significant gap between DN’s expectation and perception.

Originality/value
Researcher suggests that employers should consider the application of a friendly interaction design and cognitive control system in creating serious games for training design.
The professional and personal values and their revelation through professional doctorates
Authors: Gibbs Paul , Maguire Kate 
Published: July 2016

Purpose– The purpose of this paper is to discuss the relationship between individual practitioners’ personal values and their developing professional agentic values. It considers how the former might be in tension with the prescribed forms of practice held to be “professional” by professional bodies, warranting membership, and indeed, any licence to practice.Design/methodology/approach– The paper seeks an understanding of the different personal and collective ontological stances and tensions that practitioners may experience as they progress through their careers, attempting to align their own values with those of the collective values within their profession. It is a conceptual paper.Findings– The authors explore the ideas through a Heideggerian reading of transdisciplinarity which the authors find helpful.Research limitations/implications– This is a conceptual paper and my therefore may suffers from lack of empirical evidence which the authors would consider helpful as the next stage of developmentPractical implications– Through the lens of an “I” and “we” framework introduced in the paper and the use of a professional doctorate, the authors discuss how a practitioner and profession’s values may be in tension.Social implications– There may be issues of professional engagement which will impact on the development of the professions themselves.Originality/value– The authors believe this to be an original approach to understanding professional and personal values in professional doctorates
Eye centre localisation: an unsupervised modular approach
Authors: Zhang Wenhao , Smith Melvyn Lionel , Smith Lyndon Neal , Farooq Abdul Rehman 
Published: July 2016

Purpose
This paper aims to introduce an unsupervised modular approach for eye centre localisation in images and videos following a coarse-to-fine, global-to-regional scheme. The design of the algorithm aims at excellent accuracy, robustness and real-time performance for use in real-world applications.

Design/methodology/approach
A modular approach has been designed that makes use of isophote and gradient features to estimate eye centre locations. This approach embraces two main modalities that progressively reduce global facial features to local levels for more precise inspections. A novel selective oriented gradient (SOG) filter has been specifically designed to remove strong gradients from eyebrows, eye corners and self-shadows, which sabotage most eye centre localisation methods. The proposed algorithm, tested on the BioID database, has shown superior accuracy.

Findings
The eye centre localisation algorithm has been compared with 11 other methods on the BioID database and six other methods on the GI4E database. The proposed algorithm has outperformed all the other algorithms in comparison in terms of localisation accuracy while exhibiting excellent real-time performance. This method is also inherently robust against head poses, partial eye occlusions and shadows.

Originality/value
The eye centre localisation method uses two mutually complementary modalities as a novel, fast, accurate and robust approach. In addition, other than assisting eye centre localisation, the SOG filter is able to resolve general tasks regarding the detection of curved shapes. From an applied point of view, the proposed method has great potentials in benefiting a wide range of real-world human-computer interaction (HCI) applications.
Proteome analysis of Pseudomonas putida F1 genes induced in soil environments
Authors: Morimoto Hajime , Kadoya Ryosuke , Takahashi Kazuhiro , Kasahara Yasuhiro 
Published: July 2016

SummaryKnowledge of the gene expression dynamics of a single soil bacterial strain contributes to the understanding of its behaviour, physiological state and surrounding microenvironment. Genes expressed in soil environments rather than in laboratory media are considered to particularly relevant. Here, we compared genome‐wide gene expression profiles of the bacterium Pseudomonas putida F1 inoculated in three different types of nonsterile soils deduced using proteome analysis via sodium dodecyl sulfate‐polyacrylamide gel electrophoresis combined with liquid chromatography‐tandem mass spectrometry. Proteins commonly detected in all three samples and involved with bacterial growth and fundamental metabolism were excluded. Nine proteins were identified as specifically expressed in soil including an aldehyde dehydrogenase, a nitric oxide dioxygenase and five proteins encoded by a cluster of metabolism‐associated genes. Expression factor analysis revealed that the nitric oxide dioxygenase‐coding gene was induced by nitric oxide and the five clustered genes were induced under phosphate starvation. The expression of these genes can be attributed to response to soil environmental stimuli surrounding the F1 cells. These results strongly suggest that our soil metaproteome approach is useful for understanding the autecology and lifestyle of a single bacterial strain in soil environments and allows the prediction of the microenvironment surrounding the bacterial cells.
Expression of the complement system's activation factors in plasma of patients with early/late‐onset severe pre‐eclampsia
Authors: He Yingdong , Xu Bingning , Song Di , Yu Feng , Chen Qian , Zhao Minghui 
Published: July 2016

ProblemTo investigate the expression of complement activation factors in plasma of patients with early/late‐onset severe pre‐eclampsia.MethodsA case–control study was performed. The study group consisted of 30 cases of early‐onset severe pre‐eclampsia (EOSPE) and 30 cases of late‐onset severe pre‐eclampsia (LOSPE). Thirty cases were selected as the early‐onset control group (E‐control) and 30 as the late‐onset control group (L‐control). ELISA was used to test C1q, C4d, MBL, Bb, C3a, C5a, and MAC in the maternal peripheral circulation.ResultsThe complement activation factors Bb, C3a, C5a, and MAC were increased significantly in EOSPE (all P<.01) and LOSPE (P value: .027, <.001, .001, and <.001, respectively) compared with E/L‐control. C1q and C4d were increased significantly in LOSPE (P value: .003 and .014, respectively) compared with L‐control.ConclusionAbnormal activation of the complement system exists in the maternal circulation of patients with early‐ and late‐onset severe pre‐eclampsia. In patients complicated with LOSPE, the complement system was activated through both the classical and alternative pathways, while in EOSPE, the complement system was activated mainly through the alternative pathway.
Effect of uterine contractions on fetal heart rate in pregnancy: a prospective observational study
Authors: Sletten Julie , Kiserud Torvid , Kessler Jörg 
Published: July 2016

AbstractIntroductionThe new Holter monitoring technology enables long‐term electrocardiographic recording of the fetal heart rate without discomfort for the mother. The aim of the study was to assess the feasibility of a fetal Holter monitor. This technology was further used to study fetal heart rate outside the hospital setting during normal daily activities and to test the hypothesis that uterine activity during pregnancy influences fetal heart rate.Material and methodsProspective observational study including 12 healthy pregnant women at 20–40 weeks of gestation. Data were collected using the Monica AN24 system. Outcome measures were fetal heart rate, maternal heart rate, and uterine activity categorized according to the strength of the electrohysterographic signal.ResultsThe recordings had a median length of 18.8 h, and fetal heart rate and maternal heart rate were obtained with success rates of 73.1 and 99.9%, respectively. Uterine activity was found to affect fetal heart rate in all participants. Compared with the basal tone and mild levels of uterine activity, moderate and strong levels of uterine activity were associated with increases in fetal heart rate of 4.0 and 5.7 beats/min, respectively. At night, the corresponding increases were 4.9 and 7.6 beats/min. Linear correlations were found between maternal heart rate and fetal heart rate in 11 of the 12 cases, with a mean coefficient beta of 0.189. Both maternal heart rate and fetal heart rate exhibited a diurnal pattern, with lower heart rates being recorded at night.ConclusionsUterine activity during pregnancy is associated with a graded response in fetal heart rate and may represent a physiological challenge for the development and adaptation of the fetal cardiovascular system.
Impact of increased risk for fetal aneuploidy on maternal mood: a prospective longitudinal study
Authors: Nevay Dayna L. , Hippman Catriona , Inglis Angela , Albert Arianne , Austin Jehannine 
Published: July 2016

AbstractIntroductionOur goal was to prospectively compare the trajectories of depression symptoms through pregnancy and postpartum between women who received normal prenatal screening results and those whose results indicated an increased risk for fetal aneuploidy.Material and methodsWomen completed the Edinburgh Postnatal Depression Scale (EPDS) at 4‐week intervals between <26 weeks' gestation and 3 months postpartum. We categorized women into four groups: (i) negative serum screening and ultrasound results (SS−/US−, n = 103), (ii) positive serum screening/negative ultrasound results (SS+/US−, n = 42), (iii) negative serum screening/positive ultrasound results (SS−/US+, n = 19), or (iv) positive serum screening and ultrasound results (SS+/US+, n = 13), and compared EPDS scores between groups using Poisson regression.ResultsWomen who received any positive prenatal screening result had significantly higher EPDS scores during pregnancy than SS−/US− women (p = 0.002), with SS−/US+ women having the highest scores. During the postpartum, any positive screening test result was only marginally significantly associated with EPDS scores (p = 0.06), but women in the SS−/US+ group had significantly higher scores than women in the SS−/US− group (p = 0.05).ConclusionsOur data suggest that different types of prenatal screening tests may have different effects on women's moods, and that depression symptoms persist for women who have soft markers identified on ultrasound.
Endoscopic ultrasonography‐guided transmural drainage of walled‐off pancreatic necrosis: Comparison between a specially designed fully covered bi‐flanged metal stent and multiple plastic stents
Authors: Bapaye Amol , Dubale Nachiket A , Sheth Keyur A , Bapaye Jay , Ramesh Jayapal , Gadhikar Harshal , Mahajani Sheetal , Date Suhas , Pujari Rajendra , Gaadhe Ravindra 
Published: July 2016

Background and AimEndoscopic ultrasonography (EUS)‐guided drainage of walled‐off necrosis (WON) may be carried out by placement of multiple plastic stents (MPS) or specially designed fully covered bi‐flanged metal stents (BFMS). Comparative data on efficacy of these two stent types for WON drainage are limited. This retrospective study compares outcomes of WON drainage using BFMS and MPS.MethodsDuring a 10‐year period, 133 patients underwent EUS‐guided WON drainage. MPS or BFMS were placed in a WON cavity through a single puncture, and direct endoscopic necrosectomy (DEN) was carried out whenever clinically necessary. Data in the two cohorts were retrospectively compared for primary outcomes – clinical success, adverse events and mortality; and secondary outcomes – DEN requirement, mean DEN sessions, need for salvage surgery and hospital stay.ResultsMPS were placed in 61 and BFMS in 72 patients. Patients undergoing BFMS drainage required fewer DEN sessions (mean 1.46 vs 2.74, P < 0.05), had fewer adverse events (5.6% vs 36.1%, P < 0.05), needed salvage surgery less often (2.7% vs 26.2%, P < 0.05), and had significantly shorter hospital stay (4.1 vs 8 days, P < 0.05) compared to those undergoing MPS drainage. There was no difference in DEN requirement (P = 0.217) and mortality (P = 0.5) in both groups. Overall clinical success with BFMS was superior to MPS (94% vs 73.7%, P < 0.05).ConclusionBFMS appear to be superior to MPS for EUS‐guided WON drainage in terms of clinical success, number of DEN sessions, adverse events, need for salvage surgery and hospital stay.
An exploration of the relationship between police presence, crime and firms in developing countries
Authors: Islam Asif 
Published: July 2016

Economic theory predicts that a rise in police presence will reduce criminal activity. However several studies in this field have found mixed results. This study adds to the literature by exploring the relationship between the size of the police force and crime experienced by firms. Using survey data for about 12,000 firms in a cross‐section of 27 developing countries it is found that increasing the size of the police force is negatively associated with crime experienced by firms. Results are confirmed using a panel of firms for a subset of countries for which data are available. It is also found that this negative relationship is stronger under certain macroeconomic circumstances.
The societal cost of Huntington's disease: are we underestimating the burden?
Authors: Jones C. , Busse M. , Quinn L. , Dawes H. , Drew C. , Kelson M. , Hood K. , Rosser A. , Edwards R. T. 
Published: July 2016

Background and purposeApproximately 9000 people in the UK are affected by Huntington's disease (HD). People with HD require ongoing health and social care support. There is a knowledge gap about costs of health and social care use associated with HD in the UK. This paper estimates the economic cost in the UK.MethodsData on UK patients for the year 2013 were extracted from the European Huntington's Disease Network REGISTRY study, a full clinical dataset, including the full medical history and medication history for patients with HD. National unit costs for the price year 2013 were applied to health and social care services.ResultsData were available for 131 people. The mean annual cost per person with HD was £21 605. The largest proportion of this cost (65%) was due to informal care (£14 085).ConclusionsInformal care was the largest driver of costs across all stages of HD; thus there is a need to also consider the needs of carers when planning services for people with HD.