bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms and Therapeutic Strategies for Pulmonary Fibrosis Post-COVID-19 ARDS: Insights from Comprehensive Bioinformatics
Authors: Li Daixi , Zheng Naishan , Zeng Huiqing 
Published: October 2024

Abstract

Background
Coronavirus disease 2019 (COVID-19) pandemic has led to numerous cases of acute respiratory distress syndrome (ARDS), with a significant number of survivors developing pulmonary fibrosis as a chronic sequela. This condition poses severe long-term health challenges, significantly burdening public health systems. Despite significant research on the acute phase of COVID-19, the mechanisms underlying pulmonary fibrosis following COVID-19 associated ARDS remain poorly understood, and effective therapies are yet to be established. This study aims to elucidate the molecular mechanisms, identify potential biomarkers, and explore therapeutic options for pulmonary fibrosis post-COVID-19-related ARDS through comprehensive transcriptomic and bioinformatic analyses.
Methods
We collected datasets from Gene Expression Omnibus (GEO) database, including transcriptional profiles of COVID-19, ARDS, and pulmonary fibrosis. Differentially expressed genes (DEGs) common to these conditions were identified, reflecting the transcriptional landscape of pulmonary fibrosis post-COVID-19 ARDS. Functional and pathway enrichment analyses was conducted. Protein-protein interaction (PPI) network was constructed to determine the hub genes and their regulatory networks. Drugs that interact with hub genes were explored and gene-disease associations were analyzed to identify potential therapeutic strategies.
Results
We identified 116 common DEGs among COVID-19, ARDS, and pulmonary fibrosis datasets. Functional enrichment highlighted critical processes including inflammatory response, apoptosis, transcription regulation, and MAPK cascade. PPI network revealed hub genes which may play crucial roles in the pathogenesis of pulmonary fibrosis post-COVID-19-related ARDS. Notably, FCER1A, associated with immune response and inflammation, GATA2, involved in macrophage function and erythropoiesis, and CLC, indicative of eosinophil activity, emerged as central players. Regulatory network analysis highlighted significant transcription factors (TFs) and microRNAs (miRNAs) associated with hub genes. We found FDA-approved drugs that could interact with these hub genes, including omalizumab, mizolastine, desloratadine, epoetin alfa, and moxidectin. Gene-disease interaction analysis revealed that diseases caused by GATA2 deficiency and immunodeficiency were associated with hub genes.
Conclusion
Our findings provide valuable insights into the molecular underpinnings of pulmonary fibrosis post-COVID-19 ARDS and highlight potential biomarkers and therapeutic targets. The repurpose of drugs offers a promising avenue for rapid clinical application, potentially improving outcomes. This study provides ideas for improved treatment for pulmonary fibrosis post-COVID-19 ARDS.
S100A4 contributes to colorectal carcinoma aggressive behavior and to chemoradiotherapy resistance in locally advanced rectal carcinoma
Authors: Harada Yohei , Ikeda Sayoko , Kawabe Yuna , Oguri Yasuko , Hashimura Miki , Yokoi Ako , Sida Akiko , Fukagawa Naomi , Hayashi Misato , Ono Mototsugu , Kusano Chika , Takahashi Hiroyuki , Saegusa Makoto 
Published: October 2024

Abstract

To investigate the functional role of S100A4 in advanced colorectal carcinoma (Ad-CRC) and locally advanced rectal carcinoma (LAd-RC) receiving neoadjuvant chemoradiotherapy (NCRT). We analyzed histopathological and immunohistochemical sections from 150 patients with Ad-CRC and 177 LAd-RC patients treated with NCRT. S100A4 knockout (KO) HCT116 cells were also used. S100A4 expression was absent in normal mucosa but increased progressively from colorectal adenoma to carcinoma, suggesting that S100A4 regulation is in an early event in colorectal carcinogenesis. In Ad-CRC, high S100A4 expression correlated with high tumor budding and nuclear b-catenin, deep invasion, lymph-vascular involvement, and unfavorable prognosis. In NCRT-treated LAd-RC, high S100A4 expression was associated with poor treatment response and short progression-free survival. S100A4 KO decreased the proliferation of HCT116 cells through activation of the p53/p21waf1 axis, and sensitized cells to adriamycin-induced apoptosis. Levels of the apoptotic marker, cleaved poly (ADP-ribose) polymerase 1, were significantly higher in samples with low S100A4 and wild type p53. Finally, we observed a direct interaction between S100A4 and p53. In conclusion, S100A4 expression engenders aggressive behavior in Ad-CRC through association with b-catenin-driven tumor buddings. S100A4 exerts anti-apoptotic and proliferative effects via inhibition of p53 in LAd-RC patients receiving NCRT, which leads to chemoradioresistance and poor prognosis.
Early Lymphocytic Recovery After Allogeneic Transplation of Hemopoetic Stem Cells in Patients with Hematologic Malignancies – Single Center Experience
Authors: Kostadinova Kameliya , Venkov Krasen , Tonev Ivan , Lazarova Yonka , Naseva Emilia , Mihaylov Georgi 
Published: October 2024

Abstract

Allogeneic hematopoietic stem cell transplantation (AlloHSCT) is a therapeutic method for the treatment of many malignant and non-malignant hematological diseases but is still associated with significant morbidity and mortality. Disease relapse recurrence and non-relapse mortality (NRM) remain the main causes of failure from AlloHSCT. The identification of the risk factors associated with this continue to be a subject of extensive scientific research. The aim of our study was to identify the prognostic significance of the early lymphocyte recovery (ELR), presented as absolute lymphocyte count (ALC) for the outcome of AlloHSCT.
Clinical application of mobile CT combined with basic anesthesia in the preoperative localization of multiple pulmonary nodules
Authors: Siyang JIAO , SHAO Feng , ZHANG Qiang , Yungang SUN 
Published: October 2024

Abstract

Objective
To evaluate the safety and efficacy of mobile CT combined with basic anesthesia for the preoperative localization of multiple nodules.
Methods
The clinical data of 200 patients who underwent CT-guided localization before single-port thoracoscopic pulmonary lobe surgery at our hospital from July 2023 to September 2023 were retrospectively analyzed. The patients were divided into two groups according to the localization method: Group A consisted of 100 patients who were localized under local anesthesia, and Group B consisted of 100 patients who were localized under basic anesthesia combined with local anesthesia. The general clinical data and localization data of the two groups were compared and analyzed.
Results
The incidence of localization complications in Group B was significantly lower than that in Group A (4% vs. 13%). The localization success rate in Group B was significantly greater than that in Group A (98% vs. 92%). The localization time in Group B was significantly shorter than that in Group A (15.23 ± 5.96 min vs. 19.90 ± 8.66 min), and the pain score in Group B was significantly lower than that in Group A (2.01 ± 2.09 min vs. 3.29 ± 2.54 min).
Conclusion
Compared with traditional local anesthesia, mobile CT combined with basic anesthesia for preoperative puncture localization of multiple pulmonary nodules results in a shorter localization time, fewer complications, greater safety, a greater success rate, and greater patient comfort, indicating that this method is safe and effective and has significant clinical value.
Effects of Mass Ratio and Restoring Condition on the Flow-Induced Vibration of Floating Cylinders with Low Aspect Ratio
Authors: Gontarski Aline Leal de Lima , Gomes Igor Rosa Ferreira , de Medeiros Melissa Muniz , Gonçalves Rodolfo Trentin , Fujarra André Luís Condino 
Published: October 2024

Abstract

The phenomenon of flow-induced vibration (FIV) generally acts on a floating body, being decisively affected by the relationship between its structural mass and the mass of the displaced fluid, which in turn is intrinsically affected by the nature of the mooring system. In this sense, solutions in stable equilibrium on the free surface are possible according to floating bodies restored by moorings, for which the mass ratio (ratio of the vibrating mass to the displaced mass in the fluid) is approximately unitary (m^* \approx 1), or for those maintained by vertically tensioned tendons that determine considerably lower mass ratios (m^* < 1). This study seeks to understand the fundamentals of the FIV responses due to different values of the mass ratio in floating cylinders with external diameter of 75mm and aspect ratio L/D=6.8. The experiments were carried out in a circulating water channel considering 12 reduced velocities for values of the mass ratio m^*=0.30, 0.60, 0.70, 0.80, 0.85, 0.90, 1.00. The floating cylinder was connected to the bottom of the channel using a tensioned tendon. Data acquisition was performed using a motion capture system that recorded the six degrees of freedom of the floating cylinder. The FIV responses provide valuable information on dynamic behaviour with respect to the structure mass and the mooring system, assessed by analysing trajectories, displacements, inclination, rotation, and oscillation frequencies. The results have shown how the significant amplitudes increase as the reduced velocity increases, similarly to cylinders with high aspect ratio. As the tendon allows the cylinder to rotate, the yaw rotation is significant for all the cases tested and contributes to amplitudes in the cross-flow direction higher than 1.5D. Knowing that floating offshore wind turbines (FOWT) are a trend for deep water generation, custom designed according to each site, this work brings important subsidies that can contribute to the selection of mooring systems, particularly with respect to the effects of the FIV phenomenon.
Establishment and validation of a predictive model for immune reconstitution in people with HIV after antiretroviral therapy
Authors: Li Na , Li Rui , Zheng Hong-Yi , He Wen-Qiang , Duan Ru-Fei , Li Xia , Tian Ren-Rong , Li Hui-Qin , Dong Xing-Qi , Shen Zhi-Qiang , Zheng Yong-Tang 
Published: October 2024

Abstract

Background
Achieving complete immune reconstitution (CIR) in people with human immunodeficiency virus (PWH) following antiretroviral therapy (ART) is essential for preventing acquired immunodeficiency syndrome (AIDS) progression and improving survival. However, there is a paucity of robust prediction models for determining the likelihood of CIR in PWH after ART. We aimed to develop and validate a CIR prediction model utilizing baseline data.
Methods
Data including demographic information, immunological profiles, and routine laboratory test results, were collected from PWH in Yunnan, China. The participants were divided into training and validation sets (7:3 ratio). To construct the model and accompanying nomogram, univariate and multivariate Cox regression analyses were performed. The model was evaluated using the C-index, time-dependent receiver operating characteristic (ROC) curves, calibration curves, and clinical decision curves to assess discrimination, calibration, and clinical applicability.
Results
5 408 PWH were included, with a CIR of 38.52%. Cox regression analysis revealed various independent factors associated with CIR, including infection route, marital status, baseline CD4+ T cell count, and baseline CD4/CD8 ratio. A nomogram was formulated to predict the probability of achieving CIR at years 4, 5, and 6. The model demonstrated good performance, as evidenced by an AUC of 0.8 for both sets. Calibration curve analysis demonstrated a high level of agreement, and decision curve analysis revealed a significant positive yield.
Conclusions
This study successfully developed a prediction model with robust performance. This model has considerable potential to aid clinicians in tailoring treatment strategies, which could enhance outcomes and quality of life for PWH.
Feasibility and Radial Artery Occlusion Rate of Sheathless Distal Transradial Access Using Balloon Guide Catheters
Authors: Onodera Koki , Yoshimura Masataka , Azekami Kuya , Kimura Ryutaro , Yahagi Noriyuki , Kajimoto Ryuta , Kohyama Shinya 
Published: October 2024

Abstract

Objectives
Distal transradial access (dTRA), performed through an anatomical snuffbox, minimizes post-procedural burdens of endovascular treatments. However, despite the benefits of balloon-guide catheters (BGCs), their use in dTRA is limited by their small radial artery diameter. Herein, we evaluated the feasibility and radial artery occlusion (RAO) rate of 8Fr BGCs used in sheathless dTRA.
Materials and Methods
This retrospective study reviewed patients treated with sheathless dTRA using an 8Fr Optimo at a single center between July 2023 and May 2024. dTRA procedures were performed under general anesthesia in patients not requiring urgent treatment. The RAO was assessed using ultrasonography 24 h after the procedure. The demographic and procedural characteristics were compared between the radial artery patency and occlusion groups.
Results
Of 170 patients, 50 underwent dTRA, and 43 (86%) completed the procedure. RAO occurred in 12/43 (28%) patients with dTRA. Univariate and receiver operating characteristic curve analyses demonstrated that the median radial artery diameter was significantly smaller in the RAO group (P < 0.001), with an optimal cut-off value of 2.4 mm to predict RAO. Complications included minor cerebral ischemia in two patients, but no severe ischemia was observed.
Conclusions
Sheathless dTRA using an 8Fr Optimo BGC is feasible, but the risk of RAO should be noted, particularly in patients with small radial artery diameters. This study suggests a radial artery diameter cutoff value of 2.4 mm to predict RAO, aiding access decisions for large-bore BGC. Further multicenter prospective studies are warranted to confirm these findings and assess long-term outcomes.
Multiple Organ Scoring Systems for Predicting In-Hospital Mortality of Sepsis Patients in the Intensive Care Unit
Authors: Zhou Xuan , Zhang Zhenen , Wang Huiming , Chen Pengfei 
Published: October 2024

Abstract

Objective
This study aims to comprehensively evaluate the Charlson Comorbidity Index (CCI), Logistic Organ Dysfunction System (LODS) score, Acute Physiology Score III (APS III), Simplified Acute Physiology Score II (SAPS II), and SOFA (Sequential Organ Failure Assessment) in order to provide a more scientifically rigorous and effective tool for predicting mortality risk among sepsis patients in the Intensive Care Unit (ICU).
Methods
The demographic information and sepsis-related data were extracted from the MIMIC-IV database for patients admitted to the ICU with a diagnosis of sepsis. The predictive performance of CCI, LODS, APS III, SAPS II, and SOFA scoring systems in terms of ICU mortality was evaluated by comparing receiver operating characteristic (ROC) curves. Furthermore, multivariate regression analysis was conducted to identify potential predictors which were subsequently validated using a nomogram. Finally, the dataset was divided into a training set and validation set at a ratio of 7:3 to assess the clinical utility of the prediction model through ROC curves.
Results
The study enrolled a total of 17,226 patients with a median age of 67.65 (55.79, 78.82) years; among them, males accounted for 57.69%. Within this cohort, a total of 1,115 (6.47%) individuals succumbed during their admission to the ICU. The ROC curve demonstrated that both APS III and LODS score exhibited robust predictive value for ICU mortality. Multivariate regression analysis revealed that CCI, APS III score, LODS score, respiratory rate, body temperature, and race served as potential predictors. The integration of these indicators into a nomogram demonstrated significant clinical relevance, as evidenced by an area under the curve (AUC) of 0.803 in the training set and 0.797 in the validation set.
Conclusions
Organ failure scores hold significant clinical relevance in predicting mortality among sepsis patients in the ICU. Augmenting predictive accuracy can be achieved by integrating the CCI, APS III score, LODS score, mean respiratory rate, and body temperature during the initial 24 hours following ICU admission.
Severe Cellulitis Caused by Stenotrophomonas Maltophilia in a 48-Year-Old Male with Severe Aplastic Anemia During Hematopoietic Stem Cell Transplantation: A Case Report
Authors: Guo Qian , Jiang Juan , Jiang Yaping , Zhang Yu , Zhang Xiang 
Published: October 2024

Abstract

Background: Cellulitis, a potentially severe bacterial skin infection, is typified by pain, fever, and erythema, posing diagnostic and therapeutic challenges. Hematopoietic stem cell transplantation (HSCT) is a critical treatment for severe aplastic anemia but temporarily impairs immune function, increasing the risk of opportunistic infections, including cellulitis.
Case Presentation: This case report details a 48-year-old male with severe aplastic anemia who developed cellulitis caused by Stenotrophomonas maltophilia during HSCT. The infection occurred in his right arm and was admitted to Zhejiang Provincial Hospital of Traditional Chinese Medicine in March 2024. The patient's compromised immunity post-HSCT made managing the infection particularly complex.
Conclusion: Despite the critical nature of the infection, the patient was effectively treated through a multidisciplinary approach and was discharged after 128 days. His recovery underscores the significance of early diagnosis and tailored treatment in immunocompromised patients, emphasizing the role of coordinated medical care in achieving favorable outcomes post-HSCT.
SSR4 promote gastric cancer progression by regulating mitochondrial oxidative phosphorylation via NDUFB11 and ATP6AP1
Authors: Liu Lei , Li Aoshuang , Liao Baixue , Wu Kaiwen , Fan Ruiling , Zhu Binjun , Sun Xiaobin 
Published: October 2024

Abstract

Gastric cancer (GC) is one of the most common cancer worldwide. Cancer cell metastasis is a major factor leading to poor prognosis. Previous proteomic data suggested that SSR4 might be closely associated with the occurrence and development of GC. However, the role and molecular mechanism of SSR4 in GC is not yet clear. The present study found that the expression level of SSR4 was increased in GC tissue and serum from GC patients. In addition, SSR4 could promote the malignant biological behavior of GC cells in vitro and in vivo. The mechanism may be that SSR4 regulates the expression of NDUFB11 and ATP6AP1, and then enhanced the function of mitochondrial respiratory chain complex I (CI) and mitochondrial respiratory chain complex V (CV), which promoted the mitochondrial oxidative phosphorylation and thus promoted GC progression. These findings expand the understanding of the role of SSR4 and provide a new target for the treatment of GC.
An enzymatic reaction-based phenylboronic acid-modified microporous array chip for chiral differentiation and high-throughput detection of D-amino acids
Authors: Lu Feng , Li Limao , Shen Kang , Qian Yayun , Zhang Pengfei , Yang Yan , Zhu Qunshan , Huang Yong , Yan Chunxiang , Wei Wei 
Published: October 2024

Abstract

D-amino acids (D-AAs), which are potential biomarkers, are found at considerably higher levels in the saliva of individuals with early gastric cancer (GC), making the development of a rapid and sensitive assay imperative. In this paper, a Raman-active boronate modified surface-enhanced Raman scattering (SERS) microporous array chip based on enzymatic reaction was constructed for reliable, sensitive and quantitative monitoring of D-Proline (D-Pro) and D-Alanine (D-Ala) in saliva. Initially, 3-mercaptophenylboronic acid (3-MPBA) was bonded to Au-coated Si nanocrown arrays (Au/SiNCA) via Au-S bonding. Following this, H2O2 obtained from D-Amino acid oxidase (DAAO)-specific catalyzed D-AAs further reduced 3-MPBA to 3-hydroxythiophenol (3-HTP) with a new Raman peak at 882 cm-1. Meanwhile, the original characteristic peak at 998 cm-1 remained unchanged. Therefore, the I882/I998 ratio increased as the D-AAs content in the sample to be tested rose, allowing D-AAs to be quantitatively detected. Proudly, the Au/SiNCA with large-area periodic crown structure prepared in this paper could provide numerous, uniform “hot spots”, and the microporous array chip with 16 detection units was employed as the platform for SERS analysis, realizing high-throughput, high sensitivity, high specificity and high reliability quantitative detection of D-AAs (D-Pro and D-Ala). The limits of detection (LOD) were down to 10.1 μM and 13.7 μM throughout the linear range of 20-500 μM. The good results of the saliva detection suggested that this SERS sensor could rapidly differentiate between early-stage GC patients and healthy individuals.
Association between weekend catch-up sleep and osteoarthritis: NHANES 2017-2020
Authors: Liu Yunyi , Zheng Yanxu , Tang Long , Bao Jing , Wang Jin , Liu Jingyu , Qiu Yixuan , Zhu Anding , Wu Jinze 
Published: October 2024

Abstract

Background:Osteoarthritis (OA) is a leading cause of disability, affecting millions worldwide. Emerging evidence suggests that sleep behaviors, particularly weekend catch-up sleep (WCS), may influence OA risk, though research on this association is limited.Methods:Data from the National Health and Nutrition Examination Survey (NHANES) 2017-2020 were analyzed, including 7,059 adults aged 20 and older. Weighted logistic regression models adjusted for demographic and lifestyle factors were used to examine the relationship between WCS and OA.Results:Among the participants, those with short (OR = 0.85, 95% CI 0.75-0.95), moderate (OR = 0.78, 95% CI 0.69-0.89), and long (OR = 0.80, 95% CI 0.71-0.90) WCS durations demonstrated a significantly lower prevalence of OA compared to those without WCS. The protective effect of WCS was consistent across all adjusted models. Subgroup analyses showed that this inverse association was robust across various age groups, genders, races, education levels, marital statuses, poverty income ratios (PIR), and workday sleep durations.Conclusions:This study found that weekend catch-up sleep is inversely associated with the prevalence of osteoarthritis. Adequate weekend catch-up sleep may play a role in reducing the risk of OA. These findings suggest that improving sleep patterns could be a potential strategy for OA prevention.