bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amine‐modified poly(vinyl alcohol) as a novel surfactant to modulate size and surface charge of poly(lactide‐co‐glycolide) nanoparticles
Authors: Cella Claudia , Martello Federico , Ghisletti Serena , Lenardi Cristina , Milani Paolo , Argentiere Simona 
Published: April 2016

AbstractPoly(lactide‐co‐glycolide) (PLGA) nanoparticles (NPs) represent a promising tool for effective delivery of biomacromolecules, thanks to their biodegradability and biocompatibility properties. PLGA NPs are often synthesized by the emulsion‐solvent evaporation method and poly(vinyl alcohol) (PVA) represents one of the most commonly used surfactants. Although PVA‐mediated synthesis of PLGA NPs is effective in tailoring NP size and stability, the resulting negative surface charge can prevent both endosomal escape and biomacromolecule release in cell cytosol. To overcome this limit, a novel amino‐modified PVA (amino‐PVA) surfactant with a cationic charge was synthesized and its potential for the formulation of PLGA NPs was investigated. In either single (oil‐in‐water) or double (water‐in‐oil‐in‐water) emulsion synthesis, different mixtures of PVA and amino‐PVA were studied, by monitoring their effects on NP size and surface charge. Optimized properties were obtained with a combination of 0.975% (w/v) of PVA with 0.025% (w/v) of amino‐PVA. This formulation was further investigated for degradation properties and cytocompatibility. High stability and low cytotoxicity make the system promising for the encapsulation and release of hydrophilic drugs and biomacromolecules. © 2016 Society of Chemical Industry
Disorder transitions and conformational diversity cooperatively modulate biological function in proteins
Authors: Zea Diego Javier , Monzon Alexander Miguel , Gonzalez Claudia , Fornasari María Silvina , Tosatto Silvio C. E. , Parisi Gustavo 
Published: April 2016

AbstractStructural differences between conformers sustain protein biological function. Here, we studied in a large dataset of 745 intrinsically disordered proteins, how ordered‐disordered transitions modulate structural differences between conformers as derived from crystallographic data. We found that almost 50% of the proteins studied show no transitions and have low conformational diversity while the rest show transitions and a higher conformational diversity. In this last subset, 60% of the proteins become more ordered after ligand binding, while 40% more disordered. As protein conformational diversity is inherently connected with protein function our analysis suggests differences in structure‐function relationships related to order‐disorder transitions.
Surface shapes and surrounding environment analysis of single‐ and double‐stranded DNA‐binding proteins in protein‐DNA interface
Authors: Wang Wei , Liu Juan , Sun Lin 
Published: April 2016

ABSTRACTProtein‐DNA bindings are critical to many biological processes. However, the structural mechanisms underlying these interactions are not fully understood. Here, we analyzed the residues shape (peak, flat, or valley) and the surrounding environment of double‐stranded DNA‐binding proteins (DSBs) and single‐stranded DNA‐binding proteins (SSBs) in protein‐DNA interfaces. In the results, we found that the interface shapes, hydrogen bonds, and the surrounding environment present significant differences between the two kinds of proteins. Built on the investigation results, we constructed a random forest (RF) classifier to distinguish DSBs and SSBs with satisfying performance. In conclusion, we present a novel methodology to characterize protein interfaces, which will deepen our understanding of the specificity of proteins binding to ssDNA (single‐stranded DNA) or dsDNA (double‐stranded DNA). Proteins 2016; 84:979–989. © 2016 Wiley Periodicals, Inc.
Mobility of a supported lipid bilayer on dispersed single‐walled carbon nanotubes
Authors: Posseckardt Juliane , Zhang Jiawei , Mertig Michael 
Published: April 2016

The mobility of lipid bilayers supported on single‐walled carbon nanotube (SWCNT) networks on mica has been investigated by fluorescence recovery after photobleaching (FRAP). The carbon nanotubes have been dispersed by two different bile salt derivatives (sodium taurodeoxycholate (TDOC) and sodium cholate (SC)) as well as by single‐stranded deoxyribonucleic acid (ssDNA) oligonucleotides. Whereas the diffusion coefficient of the supported lipid bilayer (SLB) is not influenced by TDOC‐dispersed SWCNTs, for SC‐ and ssDNA‐dispersed SWCNTs, it is reduced and becomes SWCNT concentration dependent. Scanning force microscopy (SFM) measurements reveal that the lipid bilayer is not affected, when spanning over TDOC‐dispersed SWCNTs, whereas SC‐ and ssDNA‐dispersed SWCNTs are only bridged by a lipid monolayer acting as diffusion barrier. The effect is more pronounced for ssDNA‐dispersed SWCNTs.Schematic viewgraph of lipid bilayer supported on a SWCNT network on mica.
The efficiency of diabatic heating and tropical cyclone intensification
Authors: Smith Roger K. , Montgomery Michael T. 
Published: April 2016

Widely held arguments attributing the increasingly rapid intensification of tropical cyclones to the increasing ‘efficiency’ of diabatic heating in the cyclone's inner core region associated with deep convection are examined. The efficiency, in essence the amount of temperature warming compared with the amount of latent heat released, is argued to increase as the vortex strengthens on account of the strengthening inertial stability. Another aspect of the efficiency ideas concerns the location of the heating in relation to the radius of maximum tangential wind speed, with heating inside this radius seen to be more efficient in rapidly developing a warm‐core thermal structure and, presumably, a rapid increase in the tangential wind.A more direct interpretation of the increased spin‐up rate is offered when the diabatic heating is located inside the radius of maximum tangential wind speed. Further, we draw attention to the limitations of assuming a fixed diabatic heating rate as the vortex intensifies and offer reasons, on these grounds alone, as to why it is questionable to apply the efficiency argument to interpret the results of observations or numerical model simulations of tropical cyclones. Moreover, since the spin‐up of the maximum tangential winds in a tropical cyclone takes place in the boundary layer and the spin‐up of the eyewall is a result of the vertical advection of high angular momentum from the boundary layer, it is questionable also whether deductions about efficiency in theories that neglect the boundary‐layer dynamics and thermodynamics are relevant to reality.
The two‐way relationship between the Madden–Julian oscillation and anticyclonic wave breaking
Authors: MacRitchie K. , Roundy P. E. 
Published: April 2016

The Madden– Julian oscillation (MJO) is the primary mode of intraseasonal variability in the Tropics. The MJO often couples with convection as it moves over the Indian Ocean and decouples as it leaves the west Pacific Ocean. The association between convection coupled to the MJO and the midlatitude flow pattern has been the primary focus of numerous studies. In particular, it has been shown that there is a statistical relationship between the position and strength of the MJO and anticyclonic wave breaking (AWB). This study expands on this statistical relationship by examining the two‐way relationship between the MJO and AWB.Using a climatology of AWB during an extended boreal cold season, we demonstrate that there is enhanced AWB over the central North Pacific when active MJO convection is over the eastern Indian Ocean and Maritime Continent. Convection associated with the MJO can induce AWB when active MJO convection is over the Indian Ocean. Once the wave breaks over the central North Pacific, the residual cut‐off low phases with the MJO circulation to enhance it as it propagates eastward, resulting in a higher amplitude MJO than when no AWB occurs.
A Novel Approach to Simultaneous Robust Design of Product Parameters and Tolerances Using Quality Loss and Multivariate ANOVA Concepts
Authors: Hazrati‐Marangaloo Hossein , Shahriari Hamid 
Published: April 2016

AbstractDesign and development of high quality products are of utmost importance to any production plant. Product design consists of parameter design and tolerance design, which affect the product performances and the manufacturing costs, respectively. Most products involve more than one quality feature. Design and development of such products raise multi‐response surface problems in which it is necessary to determine the optimal values of parameters and the tolerances for all responses simultaneously. In this research, an approach for simultaneous robust parameter and tolerance design is proposed to deal with multi‐response problems. The proposed method employs quality loss concept and one‐way multivariate analysis of variance. Two simulation studies are performed to validate the applicability of the proposed method. Research findings show that the proposed method performs better in quality improvement as well as in cost reduction than the existing methods. The variances of the responses are also lower than those of the other methods, that is, the proposed method results in a more robust approach to product design. Copyright © 2016 John Wiley & Sons, Ltd.
Bronchial‐pulmonary arterial fistula with primary racemose hemangioma
Authors: Kato Miharu , Morio Yoshiteru , Matsunaga Takeshi , Shiraishi Akihiko , Uekusa Toshimasa , Takahashi Kazuhisa 
Published: April 2016

AbstractBronchial‐pulmonary arterial fistula (BPAF) is a rare vascular malformation complicated with racemose hemangioma. We report a case of a 65‐year‐old male with BPAF with primary racemose hemangioma. Bronchial arteriography demonstrated convolution, dilation, and aneurysm connected with pulmonary artery, suggesting the presence of BPAF, in the left upper lobe. Since a 20‐mm sized aneurysm of bronchial artery and BPAF coexisted, he underwent ligation of bronchial arterial aneurysm and left upper lobectomy to prevent hemoptysis. As neither history of prior illness nor histopathologic findings of vascular inflammation was seen, the present case demonstrated BPAF with primary racemose hemangioma. Establishment of non‐invasive treatment strategy for BPAF is urgently required.
Primary ciliary dyskinesia with complex abnormalities including cleavage of B‐subfibers
Authors: Orimo Keisuke , Kondo Mitsuko , Arimura Ken , Takeyama Kiyoshi , Takeuchi Kazuhiko , Tamaoki Jun 
Published: April 2016

AbstractA 25‐year‐old Japanese woman suffered from repeated respiratory tract infections. Because of her characteristic medical history and imaging findings, we suspected primary ciliary dyskinesia (PCD) and performed a transbronchial biopsy. The biopsy revealed complex abnormalities of the ciliary structure including cleavage of the B‐subfibers observed by transmission electron microscopy analysis and the complete loss of ciliary motion by video analysis. Genetic examinations to diagnose PCD have progressed in recent years. However, in this case, the well‐known genetic mutations in causal genes of PCD were not detected via whole‐exome sequencing of the blood. Cleavage of the B‐subfibers in patients with PCD has never been reported. This case appears to be the first report of this PCD subtype in humans.
Bevacizumab‐induced chronic interstitial pneumonia during maintenance therapy in non‐small cell lung cancer
Authors: Sekimoto Yasuhito , Kato Motoyasu , Shukuya Takehiko , Koyama Ryo , Nagaoka Tetsutaro , Takahashi Kazuhisa 
Published: April 2016

AbstractBevacizumab is a monoclonal antibody targeting the vascular endothelial growth factor receptor and a key drug for advanced non‐small cell lung cancer. There are few reports describing bevacizumab‐induced chronic interstitial pneumonia.A 62‐year‐old man with advanced non‐small cell lung cancer was admitted to our hospital with dyspnea. He previously received four courses of carboplatin plus paclitaxel with bevacizumab combination therapy and thereafter received four courses of maintenance bevacizumab monotherapy. A chest‐computed tomography scan on admission revealed diffuse ground glass opacity. He had not received any other drugs and did not have pneumonia. Thus, he was diagnosed with bevacizumab‐induced chronic interstitial pneumonia and was treated with a high dose of corticosteroids. After steroid treatment, his dyspnea and radiological findings improved.This case report is the first description of bevacizumab‐induced chronic interstitial pneumonia during maintenance therapy in a patient with non‐small cell lung cancer.
Drivers of Plant Invasion at Broad and Fine Scale in Short Temperate Streams
Authors: Liendo D. , García‐Mijangos I. , Campos J. A. , López‐Muniain U. , Biurrun I. 
Published: April 2016

AbstractRiparian ecosystems have been described as highly prone to alien plant invasions; thus, disentangling the contributing factors of the invasion process is of utmost importance to conserving and managing these valuable ecosystems. In this study we examined the drivers of riparian plant invasion in 16 Cantabrian river basins (northern Spain) ranging from 100 to ca. 1050 km2. A complete flora was determined for five randomly selected sites within those basins. One hundred and thirty alien plant species were found across the 80 sampling sites, representing 21% of the recorded total flora. At site scale, the level of plant invasion, measured as alien richness (AR) and relative alien richness (RAR), was assessed in relation to a set of explanatory variables by means of Generalised Linear Mixed Models. This level of invasion was influenced by environmental variables such as the thermicity index, the average riverbed width and the number of plant communities and by human‐related variables such as the distance to the nearest town and the proportion of surrounding urban land. At basin scale, industrialised river basins were more heavily invaded than non‐industrialised basins, and they both differed in their alien plant composition. Given that some of the alien species occurring in Cantabrian streams are specially abundant (Crocosmia x crocosmiiflora) and/or form very dense stands (Fallopia japonica, Paspalum distichum), future research should focus on the drivers that influence the presence and distribution of these species of special concern. Copyright © 2016 John Wiley & Sons, Ltd.
The Influence of User Behaviour on Energy Use in European Shopping Centres
Authors: Woods Ruth , Skeie Kristian S. , Haase Matthias 
Published: April 2016

AbstractEuropean society spends increasing amounts of time in shopping centres. There is an urgent need to reduce energy use and to provide stakeholders with the tools to develop the next generation of sustainable shopping centres. The intention here is to identify how user behaviour, in particular owners, managers, tenants and customers, influences energy performance in European shopping centres. The causes of energy use are often primarily technical; but user behaviour influences whether actions to reduce energy use are implemented and how effective the actions are. Customers are not interested in energy use or sustainability issues when doing their shopping, and this influences how shopping centres act with regard to energy use reductions. This has implications for their sustainability and suggests a design potential in shopping centres, one which lies in considering social and behavioural issues alongside the implementation of more technical aspects associated with energy use in retail environments. Copyright © 2016 John Wiley & Sons, Ltd and ERP Environment
The Economic–Social Performance Relationships of Ports: Roles of Stakeholders and Organizational Tension
Authors: Cheon SangHyun 
Published: April 2016

AbstractThis study examines the mechanisms that shape relationships between port economic and social performance. Based on the concepts of organizational paradox and stakeholder heterogeneity, the study develops a paradox–stakeholder combination matrix to explain context‐ and issue‐specific economic–social performance relationships. Differing economic–social performance links may vary with different stakeholders' roles in relation to ports' sustainable development issues. The empirical evidence from US ports provides crucial implications for quasi‐public organizations, which face complex organizational identities. Copyright © 2016 John Wiley & Sons, Ltd and ERP Environment
The Kerala Tourism Model – An Indian State on the Road to Sustainable Development
Authors: Thimm Tatjana 
Published: April 2016

AbstractSustainable tourism in Kerala is on the rise. Therefore, this South Indian state is assessed according to the sustainable tourism criteria of the Strasdas et al. (2007) framework. Kerala as a state does not qualify as a sustainable tourism destination, although individual success stories at the NGO and government level exist. This conceptual paper delivers a detailed analysis of the three dimensions of sustainability, i.e. ecology, economy and socio‐cultural aspects, of the ‘Kerala tourism model’ and discusses the question of whether this model can be transferred to other developing countries. Copyright © 2016 John Wiley & Sons, Ltd and ERP Environment
PSO‐based optimal peer selection approach for highly secure and trusted P2P system
Authors: Nallakannu Senthil Madasamy , Thiagarajan Revathi 
Published: April 2016

AbstractThere is a significant growth of the peer‐to‐peer (P2P) systems over the last few years because of their high potential for sharing file among the peers. Selection of the optimal peer ensures high data transmission rate and reduced network cost. However, it is highly difficult to select the optimal peer because of the variation in the heterogeneous capacity and dynamic capacity of the peers. Efficient peer selection approach is highly required to overcome this difficulty. This paper proposes a particle swarm optimization (PSO)‐based optimal peer selection approach for a highly secure and trusted P2P system, based on the bandwidth and trust level of the peers. File replication is performed to effectively tackle the overloading of the hot files. Replacement of the replicated file is performed, based on their demand rates. The success rate and bandwidth rate of the peer are computed. Bandwidth and trust‐based PSO is applied to select the optimal peer. This improves the success rate for the peer transmission. After selecting the optimal peer, the replicated file is encrypted and transmitted to the selected peer. Finally, the replicated file is decrypted. Our proposed approach achieves minimum average query delay and ensures a highly secure and trusted environment for the P2P systems. The performance of the proposed approach is evaluated by comparing the proposed approach with the existing techniques. From the comparison results, it is clearly evident that the proposed approach achieves higher success rate and lower link stress, delay, communication overhead, finished time and network cost. Copyright © 2016 John Wiley & Sons, Ltd.
Purinergic and Store-Operated Ca2+ Signaling Mechanisms in Mesenchymal Stem Cells and Their Roles in ATP-Induced Stimulation of Cell Migration
Authors: Peng Hongsen , Hao Yunjie , Mousawi Fatema , Roger Sebastien , Li Jing , Sim Joan A. , Ponnambalam Sreenivasan , Yang Xuebin , Jiang Lin-Hua 
Published: April 2016

Abstract
ATP is an extrinsic signal that can induce an increase in the cytosolic Ca2+ level ([Ca2+]c) in mesenchymal stem cells (MSCs). However, the cognate intrinsic mechanisms underlying ATP-induced Ca2+ signaling in MSCs is still contentious, and their importance in MSC migration remains unknown. In this study, we investigated the molecular mechanisms underlying ATP-induced Ca2+ signaling and their roles in the regulation of cell migration in human dental pulp MSCs (hDP-MSCs). RT-PCR analysis of mRNA transcripts and interrogation of agonist-induced increases in the [Ca2+]c support that P2X7, P2Y1, and P2Y11 receptors participate in ATP-induced Ca2+ signaling. In addition, following P2Y receptor activation, Ca2+ release-activated Ca2+ Orai1/Stim1 channel as a downstream mechanism also plays a significant role in ATP-induced Ca2+ signaling. ATP concentration-dependently stimulates hDP-MSC migration. Pharmacological and genetic interventions of the expression or function of the P2X7, P2Y1 and P2Y11 receptors, and Orai1/Stim1 channel support critical involvement of these Ca2+ signaling mechanisms in ATP-induced stimulation of hDP-MSC migration. Taken together, this study provide evidence to show that purinergic P2X7, P2Y1, and P2Y11 receptors and store-operated Orai1/Stim1 channel represent important molecular mechanisms responsible for ATP-induced Ca2+ signaling in hDP-MSCs and activation of these mechanisms stimulates hDP-MSC migration. Such information is useful in building a mechanistic understanding of MSC homing in tissue homeostasis and developing more efficient MSC-based therapeutic applications.
Hispolon suppresses migration and invasion of human nasopharyngeal carcinoma cells by inhibiting the urokinase‐plasminogen activator through modulation of the Akt signaling pathway
Authors: Ho Hsin‐Yu , Ho Yung‐Chuan , Hsieh Ming‐Ju , Yang Shun‐Fa , Chuang Chun‐Yi , Lin Chiao‐Wen , Hsin Chung‐Han 
Published: April 2016

ABSTRACTHispolon has been reported to possess antioxidant, antiinflammatory, and antitumor activities. However, the effect of hispolon on the metastasis of nasopharyngeal carcinoma (NPC) remains unclear. In this study, we investigated how the antimetastatic activity and relevant signaling pathways of hispolon affected three NPC cell lines. The results revealed that hispolon significantly reduced the migration and invasion of three NPC cells in a dose‐dependent manner from 0 to 50 µM. Hispolon also significantly inhibited the activity and expression of urokinase‐plasminogen activator (uPA) as well as the phosphorylation of Akt. Moreover, blocking the Akt pathway also enhanced the antimetastatic ability of hispolon in the NPC cells. In conclusion, hispolon inhibited uPA expression and NPC cell metastasis by downregulating Akt signal pathways; therefore, hispolon exerts beneficial effects in chemoprevention. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 645–655, 2017.
What is new in nanoparticle‐based photoacoustic imaging?
Authors: Lemaster Jeanne E. , Jokerst Jesse V. 
Published: April 2016

Photoacoustic imaging combines the high temporal and spatial resolution of ultrasound with the good contrast and spectral tuning of optical imaging. Contrast agents are used in photoacoustic imaging to further increase the contrast and specificity of imaging or to image a specific molecular process, e.g., cell‐surface proteins or small molecule biomarkers. Nanoparticle‐based contrast agents are important tools in photoacoustic imaging because they offer intense and stable signal and can be targeted to specific molecular processes. In this review, we describe some of the most interesting and recent advances in nanoparticle‐based photoacoustic imaging including inorganic nanoparticles, organic/polymeric nanoparticles, nanoparticle coatings, multimodality imaging, as well as emerging topics in the field. WIREs Nanomed Nanobiotechnol 2017, 9:e1404. doi: 10.1002/wnan.1404This article is categorized under:

Diagnostic Tools > In Vivo Nanodiagnostics and Imaging