bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the emergence, genetic diversity and evolution of classical swine fever virus
Authors: Rios Liliam , Coronado Liani , Naranjo-Feliciano Dany , Martínez-Pérez Orlando , Perera Carmen L. , Hernandez-Alvarez Lilian , Díaz de Arce Heidy , Núñez José I. , Ganges Llilianne , Pérez Lester J. 
Published: December 2017

AbstractClassical swine fever (CSF) is one of the most important infectious diseases causing significant economic losses. Its causal agent, CSF virus (CSFV), is a member of the Pestivirus genus included into the Flaviviridae family. Previous molecular epidemiology studies have revealed the CSFV diversity is divided into three main genotypes and different subgenotypes. However, the classification system for CSFV has not yet been harmonized internationally. Similarly, the phylogeny and evolutionary dynamics of CSFV remain unclear. The current study provides novel and significant insights into the origin, diversification and evolutionary process of CSFV. In addition, the best phylogenetic marker for CSFV capable of reproducing the same phylogenetic and evolutionary information as the complete viral genome is characterized. Also, a reliable cut-off to accurately classify CSFV at genotype and subgenotype levels is established. Based on the time for the most recent common ancestor (tMRCA) reconstruction and cophylogenetic analysis, it was determined that CSFV emerged around 225 years ago when the Tunisian Sheep Virus jumped from its natural host to swine. CSFV emergence was followed by a genetic expansion in three main lineages, driven by the action of positive selection pressure and functional divergence, as main natural forces.
Shape analysis of the basioccipital bone in Pax7-deficient mice
Authors: Cates Joshua , Nevell Lisa , Prajapati Suresh I. , Nelon Laura D. , Chang Jerry Y. , Randolph Matthew E. , Wood Bernard , Keller Charles , Whitaker Ross T. 
Published: December 2017

AbstractWe compared the cranial base of newbornPax7-deficient and wildtype mice using a computational shape modeling technology called particle-based modeling (PBM). We found systematic differences in the morphology of the basiooccipital bone, including a broadening of the basioccipital bone and an antero-inferior inflection of its posterior edge in thePax7-deficient mice. We show that thePax7cell lineage contributes to the basioccipital bone and that the location of thePax7lineage correlates with the morphology most effected byPax7deficiency. Our results suggest that thePax7-deficient mouse may be a suitable model for investigating the genetic control of the location and orientation of the foramen magnum, and changes in the breadth of the basioccipital.
Seabird species vary in behavioural response to drone census
Authors: Brisson-Curadeau Émile , Bird David , Burke Chantelle , Fifield David A. , Pace Paul , Sherley Richard B. , Elliott Kyle H. 
Published: December 2017

AbstractUnmanned aerial vehicles (UAVs) provide an opportunity to rapidly census wildlife in remote areas while removing some of the hazards. However, wildlife may respond negatively to the UAVs, thereby skewing counts. We surveyed four species of Arctic cliff-nesting seabirds (glaucous gull Larus hyperboreus, Iceland gull Larus glaucoides, common murre Uria aalge and thick-billed murre Uria lomvia) using a UAV and compared censusing techniques to ground photography. An average of 8.5% of murres flew off in response to the UAV, but >99% of those birds were non-breeders. We were unable to detect any impact of the UAV on breeding success of murres, except at a site where aerial predators were abundant and several birds lost their eggs to predators following UAV flights. Furthermore, we found little evidence for habituation by murres to the UAV. Most gulls flew off in response to the UAV, but returned to the nest within five minutes. Counts of gull nests and adults were similar between UAV and ground photography, however the UAV detected up to 52.4% more chicks because chicks were camouflaged and invisible to ground observers. UAVs provide a less hazardous and potentially more accurate method for surveying wildlife. We provide some simple recommendations for their use.
Genome-wide association study identifies a missense variant at APOA5 for coronary artery disease in Multi-Ethnic Cohorts from Southeast Asia
Authors: Han Yi , Dorajoo Rajkumar , Chang Xuling , Wang Ling , Khor Chiea-Chuen , Sim Xueling , Cheng Ching-Yu , Shi Yuan , Tham Yih Chung , Zhao Wanting , Chee Miao Ling , Sabanayagam Charumathi , Chee Miao Li , Tan Nicholas , Wong Tien Yin , Tai E-Shyong , Liu Jianjun , Goh Daniel Y. T. , Yuan Jian-Min , Koh Woon-Puay , van Dam Rob M. , Low Adrian F. , Chan Mark Yan-Yee , Friedlander Yechiel , Heng Chew-Kiat 
Published: December 2017

AbstractRecent genome-wide association studies (GWAS) have identified multiple loci associated with coronary artery disease (CAD) among predominantly Europeans. However, their relevance to multi-ethnic populations from Southeast Asia is largely unknown. We performed a meta-analysis of four GWAS comprising three Chinese studies and one Malay study (Total N = 2,169 CAD cases and 7,376 controls). Top hits (P < 5 × 10−8) were further evaluated in 291 CAD cases and 1,848 controls of Asian Indians. Using all datasets, we validated recently identified loci associated with CAD. The involvement of known canonical pathways in CAD was tested by Ingenuity Pathway Analysis. We identified a missense SNP (rs2075291, G > T, G185C) in APOA5 for CAD that reached robust genome-wide significance (Meta P = 7.09 × 10−10, OR = 1.636). Conditional probability analysis indicated that the association at rs2075291 was independent of previously reported index SNP rs964184 in APOA5. We further replicated 10 loci previously identified among predominantly Europeans (P: 1.33 × 10−7–0.047). Seven pathways (P: 1.10 × 10−5–0.019) were identified. We identified a missense SNP, rs2075291, in APOA5 associated with CAD at a genome-wide significance level and provided new insights into pathways contributing to the susceptibility to CAD in the multi-ethnic populations from Southeast Asia.
STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes
Authors: Oh Mi Ri , Lee Keon Jin , Huang Mei , Kim Jin Ock , Kim Do Han , Cho Chung-Hyun , Lee Eun Hui 
Published: December 2017

AbstractStromal interaction molecule 1 (STIM1) along with Orai1 mediates extracellular Ca2+ entry into the cytosol through a store-operated Ca2+ entry (SOCE) mechanism in various tissues including skeletal muscle. However, the role(s) of STIM2, a homolog of STIM1, in skeletal muscle has not been well addressed. The present study, first, was focused on searching for STIM2-binding proteins from among proteins mediating skeletal muscle functions. This study used a binding assay, quadrupole time-of-flight mass spectrometry, and co-immunoprecipitation assay with bona-fide STIM2- and SERCA1a-expressing rabbit skeletal muscle. The region for amino acids from 453 to 729 of STIM2 binds to sarcoplasmic/endoplasmic reticulum Ca2+-ATPase 1a (SERCA1a). Next, oxalate-supported 45Ca2+-uptake experiments and various single-myotube Ca2+ imaging experiments using STIM2-knockdown mouse primary skeletal myotubes have suggested that STIM2 attenuates SERCA1a activity during skeletal muscle contraction, which contributes to the intracellular Ca2+ distribution between the cytosol and the SR at rest. In addition, STIM2 regulates Ca2+ movement through RyR1 during skeletal muscle contraction as well as SOCE. Therefore, via regulation of SERCA1a activity, STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal muscle, which makes it both similar to, yet different from, STIM1.
Evaluating single molecule detection methods for microarrays with high dynamic range for quantitative single cell analysis
Authors: Salehi-Reyhani Ali 
Published: December 2017

AbstractSingle molecule microarrays have been used in quantitative proteomics, in particular, single cell analysis requiring high sensitivity and ultra-low limits of detection. In this paper, several image analysis methods are evaluated for their ability to accurately enumerate single molecules bound to a microarray spot. Crucially, protein abundance in single cells can vary significantly and may span several orders of magnitude. This poses a challenge to single molecule image analysis. In order to quantitatively assess the performance of each method, synthetic image datasets are generated with known ground truth whereby the number of single molecules varies over 5 orders of magnitude with a range of signal to noise ratios. Experiments were performed on synthetic datasets whereby the number of single molecules per spot corresponds to realistic single cell distributions whose ground truth summary statistics are known. The methods of image analysis are assessed in their ability to accurately estimate the distribution parameters. It is shown that super-resolution image analysis methods can significantly improve counting accuracy and better cope with single molecule congestion. The results highlight the challenge posed by quantitative single cell analysis and the implications to performing such analyses using microarray based approaches are discussed.
Calcification Propensity of Serum is Independent of Excretory Renal Function
Authors: Bielesz Bernhard , Reiter Thomas , Marculescu Rodrig , Gleiss Andreas , Bojic Marija , Kieweg Heidi , Cejka Daniel 
Published: December 2017

AbstractVascular calcification is a component of cardiovascular disease, which is leading cause of death in patients with chronic kidney disease (CKD). A functional assay (T50-test) measuring the propensity of human serum to calcify associates with mortality and cardiovascular events in CKD patients. Calcification propensity is known to increase with CKD stage. We investigated whether the T50 readout is directly dependent on excretory kidney function (eGFR) or rather explained by deranged parameters of bone and mineral metabolism in the course of CKD. T50, along with markers implicated in calcification and mineral metabolism, were measured in a cross-sectional cohort of 118 patients with CKD stage 1–5. Associations of T50 with measured parameters were analysed and partial correlations performed to test to which extent the association of T50 with eGFR can be attributed to variation of these parameters. T50 correlates with eGFR, but serum levels of phosphate and calcium largely explain this association. Phosphate, magnesium, fetuin A, albumin, bicarbonate, and serum cross-laps but not Parathyroid Hormone or Fibroblast Growth Factor 23 are associated with T50 in multivariate adjusted models. These findings indicate that T50 values depend mainly on the concentration of promoters and inhibitors of calcification in serum, but not excretory kidney function.
Isoform-selective Hsp90 inhibition rescues model of hereditary open-angle glaucoma
Authors: Stothert Andrew R. , Suntharalingam Amirthaa , Tang Xiaolan , Crowley Vincent M. , Mishra Sanket J. , Webster Jack M. , Nordhues Bryce A. , Huard Dustin J. E. , Passaglia Christopher L. , Lieberman Raquel L. , Blagg Brian S. J. , Blair Laura J. , Koren John , Dickey Chad A. 
Published: December 2017

AbstractThe heat shock protein 90 (Hsp90) family of molecular chaperones regulates protein homeostasis, folding, and degradation. The ER-resident Hsp90 isoform, glucose-regulated protein 94 (Grp94), promotes the aggregation of mutant forms of myocilin, a protein associated with primary open-angle glaucoma. While inhibition of Grp94 promotes the degradation of mutant myocilin in vitro, to date no Grp94-selective inhibitors have been investigated in vivo. Here, a Grp94-selective inhibitor facilitated mutant myocilin degradation and rescued phenotypes in a transgenic mouse model of hereditary primary open-angle glaucoma. Ocular toxicities previously associated with pan-Hsp90 inhibitors were not evident with our Grp94-selective inhibitor, 4-Br-BnIm. Our study suggests that selective inhibition of a distinct Hsp90 family member holds translational promise for ocular and other diseases associated with cell stress and protein misfolding.
EGCG-mediated Protection of the Membrane Disruption and Cytotoxicity Caused by the ‘Active Oligomer’ of α-Synuclein
Authors: Yang Jee Eun , Rhoo Kun Yil , Lee Soonkoo , Lee Jong Tak , Park Jae Hyung , Bhak Ghibom , Paik Seung R. 
Published: December 2017

Abstract(−)-Epigallocatechin gallate (EGCG), the major component of green tea, has been re-evaluated with α-synuclein (αS), a pathological constituent of Parkinson’s disease, to elaborate its therapeutic value. EGCG has been demonstrated to not only induce the off-pathway ‘compact’ oligomers of αS as suggested previously, but also drastically enhance the amyloid fibril formation of αS. Considering that the EGCG-induced amyloid fibrils could be a product of on-pathway SDS-sensitive ‘transient’ oligomers, the polyphenol effect on the transient ‘active’ oligomers (AOs) was investigated. By facilitating the fibril formation and thus eliminating the toxic AOs, EGCG was shown to suppress the membrane disrupting radiating amyloid fibril formation on the surface of liposomal membranes and thus protect the cells which could be readily affected by AOs. Taken together, EGCG has been suggested to exhibit its protective effect against the αS-mediated cytotoxicity by not only producing the off-pathway ‘compact’ oligomers, but also facilitating the conversion of ‘active’ oligomers into amyloid fibrils.
Teaching Low-Income Spanish-Speaking Caregivers to Implement
EMT en Español
With Their Young Children With Language Impairment: A Pilot Study
Authors: Peredo Tatiana Nogueira , Zelaya Maria Isabel , Kaiser Ann P. 
Published: December 2017

Purpose

This study examined the effectiveness of teaching low-income Spanish-speaking caregivers of young children with language impairment a naturalistic language intervention,
EMT en Español
.

Method

A single-case, multiple-baseline, across-behaviors design replicated across 3 caregiver–child dyads was used to examine the effects of teaching core
EMT en Español
strategies. The training program utilized the
Teach-Model-Coach-Review
instructional approach to teach strategies to support children's language development in Spanish. All sessions were at home and in Spanish.

Results
Caregivers increased their use of matched turns, target talk, expansions, and a communication elicitation procedure following training on each specific skill. Additionally, caregivers generalized increased use of matched turns and target talk to an untrained activity during the intervention period and maintained their behavior 1 month after completing intervention. Two of 3 caregivers generalized their use of expansions, and 1 caregiver generalized her use of a communication elicitation procedure. Modest effects on the child's number of different words were observed for 2 of the 3 target children over the course of the intervention sessions. All 3 children demonstrated increases in total spontaneous words.

Conclusion
Spanish-speaking caregivers were able to implement naturalistic language teaching strategies with their young children with language impairment in a relatively short-term intervention.
Predicting Response to Treatment in a Tier 2 Supplemental Vocabulary Intervention
Authors: Kelley Elizabeth , Leary Emily , Goldstein Howard 
Published: December 2017

PurposeTo effectively implement a response to intervention approach, there is a need for timely and specific information about student learning in response to treatment to ensure that treatment decisions are appropriate. This exploratory study examined responsivity to a supplemental, Tier 2 vocabulary intervention delivered to preschool children with limited language abilities.MethodA secondary analysis of a cluster-randomized trial of a supplemental vocabulary intervention was conducted. Responsivity (e.g., adequate learning) to the intervention was examined, and learning in the 1st few weeks of intervention was evaluated as a possible predictor of response to intervention.ResultsUsing a criterion of learning of 20% of target vocabulary, nearly one third of participants were identified as poor responders. A 1st unit benchmark was identified that maximized the sensitivity to identification of children who were likely to respond to the intervention.ConclusionsEven for generally effective interventions, there is likely to be a substantial proportion of children who are not responsive. Learning in the 1st few weeks of intervention may be a useful indicator of appropriate response to treatment and could inform instructional decisions.
Smart mobility management in 5G heterogeneous networks
Authors: Omheni Nouri , Bouabidi Imen , Gharsallah Amina , Zarai Faouzi , Obaidat Mohammad S. 
Published: December 2017

Mobility management has always been a challenging and important issue for the next‐generation all‐IP mobile networks. Seamless mobility is one of the requirements to provide real‐time services in such networks. Proxy Mobile IPv6 (PMIPv6) has been developed by the Internet Engineering Task Force (IETF) as a network‐based mobility management protocol to support the mobility of IP devices. Although several designs have been presented in the literature to reduce the amount of PMIPv6 signalling and latency handover, they do not satisfy the real‐time application requirements. In this study, the authors first propose a novel vertical handover algorithm that helps to anticipate the handover process in an efficient manner. The suggested approach is based on PMIPv6 and IEEE 802.21 Media Independent Handover (MIH) standard. The authors also added a new MIH primitive in order to indicate that a handover is needed to be done very soon. The triggering of such process is done thanks to two proposed dynamic thresholds. According to the analytical evaluation and simulation results, the proposed method guarantees a significant reduction of the handover delay and the signalling overhead, handover blocking probability and packet loss.
Footpoints of the giant molecular loops in the Galactic center region
Authors: Riquelme D. , Amo-Baladrón M. A. , Martín-Pintado J. , Mauersberger R. , Martín S. , Burton M. , Cunningham M. , Jones P. A. , Menten K. M. , Bronfman L. , Güsten R. 
Published: December 2017

Aims. We aim to reveal the morphology, chemical composition, kinematics, and to establish the main processes prevalent in the gas at the footpoints of the giant molecular loops (GMLs) in the Galactic center region.
Methods. Using the 22-m Mopra telescope, we mapped the M−3.8+0.9 molecular cloud, placed at the footpoints of a GML, in 3-mm range molecular lines. To derive the molecular hydrogen column density, we also observed the 13CO(2 − 1) line at 1 mm using the 12-m APEX telescope. From the 3 mm observations 12 molecular species were detected, namely HCO+, HCN, H13CN, HNC, SiO, CS, CH3OH, N2H+, SO, HNCO, OCS, and HC3N.
Results. Maps revealing the morphology and kinematics of the M−3.8+0.9 molecular cloud in different molecules are presented. We identify six main molecular complexes. We derive fractional abundances in 11 selected positions of the different molecules assuming local thermodynamical equilibrium.
Conclusions. Most of the fractional abundances derived for the M−3.8+0.9 molecular cloud are very similar over the whole cloud. However, the fractional abundances of some molecules show significant difference with respect to those measured in the central molecular zone (CMZ). The abundances of the shock tracer SiO are very similar between the GMLs and the CMZ. The methanol emission is the most abundant species in the GMLs. This indicates that the gas is likely affected by moderate ~30 km s−1 or even high velocity (50 km s−1) shocks, consistent with the line profile observed toward one of the studied position. The origin of the shocks is likely related to the flow of the gas throughout the GMLs towards the footpoints.
Pointwise feedback stabilization of an Euler-Bernoulli beam in observations with time delay
Authors: Yang Kun-Yi , Wang Jun-Min 
Published: December 2017

This paper considers a one-dimensional Euler-Bernoulli beam equation where two collocated actuators/sensors are presented at the internal point with pointwise feedback shear force and angle velocity at the arbitrary position ξ in the bounded domain (0,1). The boundary x = 0 is simply supported and at the other boundary x = 1 there is a shear hinge end. Both of the observation signals are subjected to a given time delay τ ( >0). Well-posedness of the open-loop system is shown to illustrate availability of the observer. An observer is then designed to estimate the state at the time interval when the observation is available, while a predictor is designed to predict the state at the time interval when the observation is not available. Pointwise output feedback controllers are introduced to guarantee the closed-loop system to be exponentially stable for the smooth initial values when ξ ∈ (0, 1) is a rational number satisfying ξ ≠ 2l∕(2m − 1) for any integers l, m. Simulation results demonstrate that the proposed feedback design effectively stabilizes the performance of the pointwise control system with time delay.
Metastatic Osseous Pain Control: Radiation Therapy
Authors: Formenti Silvia , Kang Josephine 
Published: December 2017

AbstractMetastatic disease to the bone is a common manifestation of advanced cancer, and can result in pain, pathologic fractures, hypercalcemia, and overall functional compromise. External beam radiation is a proven, highly efficacious, and noninvasive therapy that can provide symptomatic relief from painful osseous lesions. When deciding upon the best treatment regimen, it is important to consider patient factors such as overall life expectancy, performance status, disease burden, and site of osseous metastatic pain. Determination of best treatment ideally requires multidisciplinary input from radiologists, medical oncologists, surgeons, pain management, and palliative care specialists together with radiation oncologists.
Symptomatic Fluid Drainage: Tunneled Peritoneal and Pleural Catheters
Authors: Ha Tony , Madoff David , Li David 
Published: December 2017

AbstractMalignant ascites and pleural effusion are significant contributors to patient symptoms such as shortness of breath, abdominal distension, and nausea in the setting of cancer. There are numerous methods employed to control such symptoms such as serial drainages, pleurodesis, and tunneled drainage catheters. Tunneled drainage catheters are a safe, effective, and a minimally invasive procedure to palliate the symptoms of malignant ascites and pleural effusion.