bluuebunny/crossref_metadata_2025_split huggingface dataset:
<p align="center">The radical scavenging activity of 2-2’ diphenyl -1- picrylhydrazil (DPPH) on the methanol extracts and Ethyl acetate fractions of red dragon fruit peel (<em>Hylocereus polyrhizus</em> (F.A.C.Weber) Britton dan Rose)<strong></strong></p>
Authors: Wahdaningsih Sri , Wahyuono Subagus , Riyanto Sugeng , Murwanti Retno 
Published: August 2017

<p> </p><p>Red dragon fruit (<em>H. Polyrhizus</em>) is one of the the plants that has a great potential as natural antioxidant. This study tested the activity of radical scavenging of 2-2' diphenyl -1- pikril hidrazil (DPPH) in the methanol extract, as well as in the soluble and insoluble fractions of ethyl acetate of red dragon fruit peel. This research is carried out through various stages, such as: extraction and fractionation to obtain both insoluble fraction and soluble fractions of ethyl acetate. Antioxidant activity test is conducted by the method of thin layer chromatography and spectrophotometry.<strong> </strong>Antioxidant activity test, IC<sub>50 </sub>values of methanol extract, ethyl acetate soluble fraction, and insoluble fraction of ethyl acetate had been obtained consecutively as much as 241.19 µg /mL, 8.34 µg/mL, 46.84 µg/mL. The soluble fraction of ethyl acetate had greater antioxidant activity compared to the methanol extract and the insoluble fractions of ethyl acetate.</p>
Protective role of Bacopa monnieri against Rotenone- induced Parkinson’s disease in PC 12 cell lines
Authors: Swathi Gunduluru , Ramaiah Chintha Venkata , Rajendra Wudayagiri 
Published: August 2017

<p> Parkinson disease (PD) is a chronic neurodegenerative disorder characterized by the loss of dopaminergic (DA) neurons in the substantia nigra, decreased striatal dopamine (DA) levels, and consequent extrapyramidal motor dysfunction. PC 12 cells originate from pheochromocytoma cells of rat adrenal medulla and share many common characteristics with substantia nigra cells. They produce DA, several growth factors, such as nerve growth factor, fibroblast growth factor and transforming growth factor, and express DA receptors. Hence<em>, </em>these cells are being used to study the alterations in dopaminergic neurons, in vitro, that occur during Parkinson’s disease. Since long term usage of antiparkinsonian drugs cause high incidence of pharmacoresistence and untoward side effects, attention has been paid in recent years to screen bioactive compounds from natural medicinal plants for treatment of several neurological disorders including Parkinson’s disease. Keeping in view of relative importance of natural medicinal plants, the present study is mainly focused to characterize the anti-parkinsonian effect of <em>Bacopa monnieri </em>(BM), an Indian herb which is being extensively used in Ayurveda treatments related to neurological complications. The present study was designed to assess the neurotoxicity of rotenone on DA-producing PC12 cells and explore the possible antiparkinsonian effect of BM in comparing with Levodopa (LD) (Reference control). The survivability studies of PC 12 cell-lines were analysed using MTT assay. Pre-treatment with BM extract significantly ameliorated morphological damage, cell viability, and apoptosis of PC12 cells exposed to RT. Hence BM extract can be effectively used in the treatment of PD and other related neurological disorders.</p>
In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of <em>Onopordum acanthium</em>
Authors: Habibatni Sofiane , Zohra Abdessalam Fatma , Khalida Hani , Anwar Sirajudheen , Mansi Iman , Awadh Ali Nasser A 
Published: August 2017

<p><em>Onopordum acanthium</em> (Scotch thistle) belong to Asteraceae (Compositae). <em>O. acanthium</em> is a flowering biennial plant native to Europe and Western Asia with coarse spiny leaves 20-50 cm in width with conspicuous and spiny-winged stems. We have previously reported pro-apoptotic and cytotoxic effect of <em>Onopordum acanthium</em> crude extract against glioblastoma U-373 cells. The present study was designed to evaluate the cytotoxicity, antioxidant, xanthine oxidase inhibition, anti-inflammatory, analgesic, antipyretic activity of butanolic extract of <em>Onopordum acanthium</em>. Cytotoxicity of different solvent (methanolic, butanol, chloroform and petroleum ether) extract studied by brine shrimp lethality bioassay, total flavonoid and phenolic, antioxidant, xanthine oxidase inhibition activity was studied by <em>in-vitro</em> whereas anti- inflammation studied by carrageenan-induced paw edema model, antipyretic with 20 % brew yeast injection induced pyretic model, analgesic with 1 % acetic acid induced analgesic model investigated in <em>in-vivo </em><em>in</em> wistar rats. Good antioxidant activity was found with IC50 = 134.4 µg/ml with considerable amount of total phenolic and flavonoid content. Xanthine oxidase inhibition effect was weak with IC50 = 572.9 µg/ml. Oral administration of <em>O. Acanthium</em> butanolic extract (OA) showed minimum lethality of brine shrimp nauplii henceforth OA butanolic phases was selected for further <em>in-vivo</em> studies. OA 200 and 400 mg/kg body weight decreased the oedema by 37.78 % and 40.52 %, respectively; standard aspirin 100 mg/kg decreased 42.62 % at 5th hour of Carrageenan injection. OA 200 and 400 mg/kg significantly decreased acetic acid-induced abdominal writhes when compared to standard aspirin. OA have shown dose and time dependent decrease in body temperature in yeast induced pyrexia, comparable to standard, aspirin. The present results demonstrate that OA has notable anti-inflammatory, antipyretic, analgesic activity related to presence of phenolic compounds as from literature it has been demonstrated that isolated compounds from aerial parts of <em>Onopordum acanthium </em>had strong activity in <em>in-vitro</em> assay. </p>
Role of protective Pelargonium Sidoides root extract and Curcumin on Mushroom poisoning: An Experimental study in rats
Authors: Unsal Velid , EB Kurutaş , M Güngör , Emrah Aksan M 
Published: August 2017

<p>Phalloidin is a cyclic heptapeptide containing cysteine amino acids. The toxicity of phallodin is attributed to the sulfur atom of the sulfur in the indole ring of the molecule and it is responsible for acute gastroenteritis occurring during the initial period of poisoning. Pelargonium sidoides, reinforcing the immune system, antiviral, has antibacterial properties as well as antioxidant properties.The aim of this study is to investigate the protective role of Pelargonium sidoides and Curcumin against mushroom poisoning.</p><p>28 Wistar albino rats were divided into four groups. Group I; Along the study, 0.2 ml saline was administered intraperitoneally to the rats .Group II (phalloidin, 0.5 mg / kg) were administered for 5 days of study. Group III : From the beginning to the end of the study, 0.5 mg / kg phalloidin i.p.,0,2 ml/kg pelargonium sidoides were administered orally. Group IV : From the beginning to the end of the study, 0.5 mg / kg phalloidin intraperitoneally,0.2 ml/kg Curcumin were administered orally.At the end of the study, the rats were sacrificed. The blood of the rats was taken. İn serum Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px), Paraxonase (PON), Arylesterase (ARE) ,Adenosine Deaminase (ADA) , Xhanthine oxidase (XO),Protein Karbonyl (PC), Malondialdehyde(MDA), Nitric oxide (NO), were measured by spectrophotometry.</p><p>In Group II, MDA, , PC levels and XO activity increased significantly compared to Group I. (p&lt;0.05).PS and Curcumin treatment ameliorated some enzyme levels (SOD, GSH-px, PON, ADA ) in serum with phalloidin induced rats. Values of Group III approached Group I.</p><p>Phalloidin increases toxic ROS. Pelargonium sidoides and Curcumin are antioxidant, antitoxic. And They such as silibin can protective against mushroom poisoning.</p><p> </p>
Anti-leukemic activity of phosphoproteins from Sesamin via induction of nuclear antigen H731and CLIP-associating protein 2 isoform X25 mediated apoptosis
Authors: Wannapruk Pattharin , Paemanee Atchara , Roytrakul Sittiruk , Tanyong Dalina I 
Published: August 2017

<p>Leukemia is an uncontrolled proliferation hematopoietic cancer cells that commonly treats with conventional therapies such as chemotherapy. However, many side effects were reported. Alternative medicines have been developed by using natural or herb compounds as therapeutic drug. Sesamin, a class of phytoestrogen isolated from sesame seed displaying potent anticancer activity in <em>vitro</em> and in <em>vivo</em>.However, the mechanism by which <em>sesamin</em> mediates <em>anticancer</em> effects on leukemic cells are notfully understood. In thisstudy, the effects of sesamin on cell viability, cell apoptosis and expression of phosphoproteins in Molt-4 and NB4 leukemic cell lines were investigated using MTT assay, flow cytometry and immobilized metal affinity chromatography (IMAC) phosphoprotein enrichment and LC-MS, respectively. The results showed that sesamin reduced viability and induced apoptosis in leukemic cells. In addition, 79 phosphoproteins were identified from LC-MS within three main clusters including biological regulation, cellular processand metabolic process. Interestingly, nuclear antigen H731 (PDCD4) and CLIP-associating protein 2 isoform X25 (CLASP2) showed increased in sesamin treated cells and associated protein-ligand interaction network with allicin, capsaicin, cucurbitacin B, and rapamycin which are known to activate <em>apoptosis</em> in <em>cancer cells</em>.Then, sesamin could be developed as candidate of alternative leukemia treatment in the future.</p>
Mapeando o Pouso Frio: dinâmicas de construção de um bairro na cidade de Toledo/PR
Authors: Pereira Maria Cristina de Castro 
Published: August 2017

<p align="justify"> </p><p class="Standard">Este artigo descreve e problematiza o processo histórico de constituição de um bairro – o Pouso Frio – na cidade de Toledo, Oeste do Paraná, a partir da década de 1940. Inicialmente uma área de ocupação, esse espaço foi construído por diferentes sujeitos, em sua maioria, vindos do Norte do Paraná, São Paulo, Minas Gerais e Paraguai, ocultados na História Oficial elaborada para o município. Nas primeiras décadas em que viveram no bairro, enfrentaram o medo sempre presente de perder seus parcos investimentos em terrenos sem escrituração. Ainda conviveram, por exemplo, com a ausência de energia elétrica, da água encanada, do transporte público e da rede de esgoto. Os diálogos estabelecidos com moradores do Pouso Frio permitiram delinear um conjunto de trajetórias e expectativas que os aproximaram e os uniram na luta por melhores condições de moradia e acesso à cidade.</p><p align="justify"><span style="font-family: 'Times New Roman', serif;"><span><br /></span></span></p>
Simulated bacterial infection disrupts the circadian fluctuation of immune cells in wrinkle-lipped bats (Chaerephon plicatus)
Authors: Weise Philipp , Czirják Gábor A. , Lindecke Oliver , Bumrungsri Sara , Voigt Christian C. 
Published: August 2017

BackgroundLeukocyte concentrations follow a circadian pattern in mammals, with elevated values at times of potential contact with pathogens and parasites. We hypothesized that this pattern is disturbed after an immune challenge.MethodsIn Thailand, we captured wrinkle-lipped bats (Chaerephon plicatus), when they returned to their colony at dawn. We challenged half of the animals (experimental group) with bacterial lipopolysaccharides and treated the others only with the carrier liquid (control group). We then compared body mass changes and differences in circulating immune cell counts at 8 h post-treatment.ResultsIn experimental animals, we observed an increase in total leukocyte and neutrophil numbers of 17% and 95%, respectively. In control animals, concentrations of leukocytes decreased by 44% and those of neutrophils remained constant. Experimental treatment had no effect on lymphocytes, yet changes in eosinophil numbers were explained by sex. Eosinophils decreased by 66% in females and by 62% in males. Basophils and monocytes were rarest among all observed cell types and analysis was either impossible because of low numbers or yielded no significant effects, respectively.DiscussionOur findings show that a simulated bacterial infection triggered a neutrophil-associated immune response in wrinkle-lipped bats, indicating a disruption of the diurnal fluctuation of immune cells. Our study suggests that bats exhibit circadian rhythms in immune cell counts. The magnitude of these fluctuations may vary across species according to specific-specific infection risks associated with colony sizes or specific roosting habits.
Studying primate cognition in a social setting to improve validity and welfare: a literature review highlighting successful approaches
Authors: Cronin Katherine A. , Jacobson Sarah L. , Bonnie Kristin E. , Hopper Lydia M. 
Published: August 2017

BackgroundStudying animal cognition in a social setting is associated with practical and statistical challenges. However, conducting cognitive research without disturbing species-typical social groups can increase ecological validity, minimize distress, and improve animal welfare. Here, we review the existing literature on cognitive research run with primates in a social setting in order to determine how widespread such testing is and highlight approaches that may guide future research planning.Survey MethodologyUsing Google Scholar to search the terms “primate” “cognition” “experiment” and “social group,” we conducted a systematic literature search covering 16 years (2000–2015 inclusive). We then conducted two supplemental searches within each journal that contained a publication meeting our criteria in the original search, using the terms “primate” and “playback” in one search and the terms “primate” “cognition” and “social group” in the second. The results were used to assess how frequently nonhuman primate cognition has been studied in a social setting (>3 individuals), to gain perspective on the species and topics that have been studied, and to extract successful approaches for social testing.ResultsOur search revealed 248 unique publications in 43 journals encompassing 71 species. The absolute number of publications has increased over years, suggesting viable strategies for studying cognition in social settings. While a wide range of species were studied they were not equally represented, with 19% of the publications reporting data for chimpanzees. Field sites were the most common environment for experiments run in social groups of primates, accounting for more than half of the results. Approaches to mitigating the practical and statistical challenges were identified.DiscussionThis analysis has revealed that the study of primate cognition in a social setting is increasing and taking place across a range of environments. This literature review calls attention to examples that may provide valuable models for researchers wishing to overcome potential practical and statistical challenges to studying cognition in a social setting, ultimately increasing validity and improving the welfare of the primates we study.
VLF modal interference distance and nighttime D region VLF reflection height for west‐east and east‐west propagation paths to Fiji
Authors: Chand Atishnal Elvin , Kumar Sushil 
Published: August 2017

AbstractVery low frequency (VLF) signals from navigational transmitters propagate through the Earth‐ionosphere waveguide formed by the Earth and the lower conducting ionosphere and show the pronounced minima during solar terminator transition between transmitter and receiver. Pronounced amplitude minima observed on 19.8 kHz (NWC transmitter) and 24.8 kHz (NLK transmitter) signals recorded at Suva (18.149°S, 178.446°E), Fiji, during 2013–2014, have been used to estimate the VLF modal interference distance (DMS) and nighttime D region VLF reflection height (hN). The NWC transmitter signal propagates mostly in west‐east direction, and the NLK transmitter follows a transequatorial path propagating significantly in the east‐west direction. The values of DMS calculated using midpath terminator speed are 2103 ± 172 km and 2507 ± 373 km for these paths having west‐east and east‐west components of VLF subionospheric propagation, respectively, which agree with previously published results and within 10% with theoretical values. We have also compared the DMS estimated using a terminator time method with that calculated using terminator speed for a particular day and found both the values to be consistent. The hN values were found to be maximum during winter of Southern Hemisphere for NWC signal and winter of Northern Hemisphere for NLK signal VLF propagation paths to Suva. The hN also shows significant day‐to‐day and seasonal variabilities with a maximum of about 10 km and 23 km for NWC and NLK signal propagation paths, respectively, which could be due to the atmospheric gravity waves associated with solar terminator transition, as well as meteorological factors such as strong lightnings.
Going electric: Incorporating marine electromagnetism into ocean assimilation models
Authors: Schnepf N. R. 
Published: August 2017

AbstractHaving a clear multidimensional picture of Earth's oceans is crucial for understanding ocean processes and how they are evolving with climate change. Ocean knowledge is currently limited by relying on in situ data. A possible source of remote data is using the electromagnetic fields produced by the ocean and detected by geomagnetic satellites. Marine electromagnetic signals largely depend on three factors: oceanic transport, the local main magnetic field, and the electrical conductivity produced by the local salinity and temperature. Thus, how can marine electromagnetic signals be utilized to enhance the multidimensional picture of Earth's ocean circulation and state?
Enrichment of dissolved silica in the deep equatorial Pacific during the Eocene‐Oligocene
Authors: Fontorbe Guillaume , Frings Patrick J. , De La Rocha Christina L. , Hendry Katharine R. , Carstensen Jacob , Conley Daniel J. 
Published: August 2017

AbstractSilicon isotope ratios (expressed as δ30Si) in marine microfossils can provide insights into silica cycling over geologic time. Here we used δ30Si of sponge spicules and radiolarian tests from the Paleogene Equatorial Transect (Ocean Drilling Program Leg 199) spanning the Eocene and Oligocene (~50–23 Ma) to reconstruct dissolved silica (DSi) concentrations in deep waters and to examine upper ocean δ30Si. The δ30Si values range from −3.16 to +0.18‰ and from −0.07 to +1.42‰ for the sponge and radiolarian records, respectively. Both records show a transition toward lower δ30Si values around 37 Ma. The shift in radiolarian δ30Si is interpreted as a consequence of changes in the δ30Si of source DSi to the region. The decrease in sponge δ30Si is interpreted as a transition from low DSi concentrations to higher DSi concentrations, most likely related to the shift toward a solely Southern Ocean source of deep water in the Pacific during the Paleogene that has been suggested by results from paleoceanographic tracers such as neodymium and carbon isotopes. Sponge δ30Si provides relatively direct information about the nutrient content of deep water and is a useful complement to other tracers of deep water circulation in the oceans of the past.
Revisiting the horizontal redistribution of water in soils: Experiments and numerical modeling
Authors: Zhuang L. , Hassanizadeh S. M. , Kleingeld P. J. , van Genuchten M.Th. 
Published: August 2017

AbstractA series of experiments and related numerical simulations were carried out to study one‐dimensional water redistribution processes in an unsaturated soil. A long horizontal Plexiglas box was packed as homogenously as possible with sand. The sandbox was divided into two sections using a very thin metal plate, with one section initially fully saturated and the other section only partially saturated. Initial saturation in the dry section was set to 0.2, 0.4, or 0.6 in three different experiments. Redistribution between the wet and dry sections started as soon as the metal plate was removed. Changes in water saturation at various locations along the sandbox were measured as a function of time using a dual‐energy gamma system. Also, air and water pressures were measured using two different kinds of tensiometers at various locations as a function of time. The saturation discontinuity was found to persist during the entire experiments, while observed water pressures were found to become continuous immediately after the experiments started. Two models, the standard Richards equation and an interfacial area model, were used to simulate the experiments. Both models showed some deviations between the simulated water pressures and the measured data at early times during redistribution. The standard model could only simulate the observed saturation distributions reasonably well for the experiment with the lowest initial water saturation in the dry section. The interfacial area model could reproduce observed saturation distributions of all three experiments, albeit by fitting one of the parameters in the surface area production term.
Spectral model for long‐term computation of thermodynamics and potential evaporation in shallow wetlands
Authors: de la Fuente Alberto , Meruane Carolina 
Published: August 2017

AbstractAltiplanic wetlands are unique ecosystems located in the elevated plateaus of Chile, Argentina, Peru, and Bolivia. These ecosystems are under threat due to changes in land use, groundwater extractions, and climate change that will modify the water balance through changes in precipitation and evaporation rates. Long‐term prediction of the fate of aquatic ecosystems imposes computational constraints that make finding a solution impossible in some cases. In this article, we present a spectral model for long‐term simulations of the thermodynamics of shallow wetlands in the limit case when the water depth tends to zero. This spectral model solves for water and sediment temperature, as well as heat, momentum, and mass exchanged with the atmosphere. The parameters of the model (water depth, thermal properties of the sediments, and surface albedo) and the atmospheric downscaling were calibrated using the MODIS product of the land surface temperature. Moreover, the performance of the daily evaporation rates predicted by the model was evaluated against daily pan evaporation data measured between 1964 and 2012. The spectral model was able to correctly represent both seasonal fluctuation and climatic trends observed in daily evaporation rates. It is concluded that the spectral model presented in this article is a suitable tool for assessing the global climate change effects on shallow wetlands whose thermodynamics is forced by heat exchanges with the atmosphere and modulated by the heat‐reservoir role of the sediments.