bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐pressure minerals in shocked meteorites
Authors: Tomioka Naotaka , Miyahara Masaaki 
Published: July 2017

AbstractHeavily shocked meteorites contain various types of high‐pressure polymorphs of major minerals (olivine, pyroxene, feldspar, and quartz) and accessory minerals (chromite and Ca phosphate). These high‐pressure minerals are micron to submicron sized and occur within and in the vicinity of shock‐induced melt veins and melt pockets in chondrites and lunar, howardite–eucrite–diogenite (HED), and Martian meteorites. Their occurrence suggests two types of formation mechanisms (1) solid‐state high‐pressure transformation of the host‐rock minerals into monomineralic polycrystalline aggregates, and (2) crystallization of chondritic or monomineralic melts under high pressure. Based on experimentally determined phase relations, their formation pressures are limited to the pressure range up to ~25 GPa. Textural, crystallographic, and chemical characteristics of high‐pressure minerals provide clues about the impact events of meteorite parent bodies, including their size and mutual collision velocities and about the mineralogy of deep planetary interiors. The aim of this article is to review and summarize the findings on natural high‐pressure minerals in shocked meteorites that have been reported over the past 50 years.
Comparative landscape genetics of two frugivorous bats in a biological corridor undergoing agricultural intensification
Authors: Cleary Katherine A. , Waits Lisette P. , Finegan Bryan 
Published: July 2017

AbstractAgricultural intensification in tropical landscapes poses a new threat to the ability of biological corridors to maintain functional connectivity for native species. We use a landscape genetics approach to evaluate impacts of expanding pineapple plantations on two widespread and abundant frugivorous bats in a biological corridor in Costa Rica. We hypothesize that the larger, more mobile Artibeus jamaicensis will be less impacted by pineapple than the smaller Carollia castanea. In 2012 and 2013, we sampled 735 bats in 26 remnant forest patches surrounded by different proportions of forest, pasture, crops and pineapple. We used 10 microsatellite loci for A. jamaicensis and 16 microsatellite loci for C. castanea to estimate genetic diversity and gene flow. Canonical correspondence analyses indicate that land cover type surrounding patches has no impact on genetic diversity of A. jamaicensis. However, for C. castanea, both percentage forest and pineapple surrounding patches explained a significant proportion of the variation in genetic diversity. Least‐cost transect analyses (LCTA) and pairwise G″st suggest that for A. jamaicensis, pineapple is more permeable to gene flow than expected, while as expected, forest is the most permeable land cover for gene flow of C. castanea. For both species, LCTA indicate that development may play a role in inhibiting gene flow. The current study answers the call for landscape genetic research focused on tropical and agricultural landscapes, highlights the value of comparative landscape genetics in biological corridor design and management and is one of the few studies of biological corridors in any ecosystem to implement a genetic approach to test corridor efficacy.
Structural characterization of the NAP; the major adhesion complex of the human pathogen Mycoplasma genitalium
Authors: Scheffer Margot P. , Gonzalez‐Gonzalez Luis , Seybert Anja , Ratera Mercè , Kunz Michael , Valpuesta José M. , Fita Ignacio , Querol Enrique , Piñol Jaume , Martín‐Benito Jaime , Frangakis Achilleas S. 
Published: July 2017

SummaryMycoplasma genitalium, the causative agent of non‐gonococcal urethritis and pelvic inflammatory disease in humans, is a small eubacterium that lacks a peptidoglycan cell wall. On the surface of its plasma membrane is the major surface adhesion complex, known as NAP that is essential for adhesion and gliding motility of the organism. Here, we have performed cryo‐electron tomography of intact cells and detergent permeabilized M. genitalium cell aggregates, providing sub‐tomogram averages of free and cell‐attached NAPs respectively, revealing a tetrameric complex with two‐fold rotational (C2) symmetry. Each NAP has two pairs of globular lobes (named α and β lobes), arranged as a dimer of heterodimers with each lobe connected by a stalk to the cell membrane. The β lobes are larger than the α lobes by 20%. Classification of NAPs showed that the complex can tilt with respect to the cell membrane. A protein complex containing exclusively the proteins P140 and P110, was purified from M. genitalium and was structurally characterized by negative‐stain single particle EM reconstruction. The close structural similarity found between intact NAPs and the isolated P140/P110 complexes, shows that dimers of P140/P110 heterodimers are the only components of the extracellular region of intact NAPs in M. genitalium.
Lithium enhances alveolar bone formation during orthodontic retention in rats
Authors: Pan J. , He S. , Yin X. , Li Y. , Zhou C. , Zou S. 
Published: July 2017

Structured AbstractObjectiveTo investigate the effect of lithium on alveolar bone formation during orthodontic retention in rats.Materials and methodsAfter 2 weeks of orthodontic tooth movement (OTM), 42 8‐week‐old male Wistar rats were randomly divided into two orthodontic retention groups: one without (control) and the other with LiCl treatment (LiCl group). Samples were collected on days 0, 3, 7 and 14 during the retention period. We evaluated the bone volume/total volume (BV/TV) ratio and new bone formation in the region of interests (ie, the root, the periodontal ligament and the adjacent alveolar bone around the distal buccal surface of the distal root of the maxillary first molar). We performed quantitative analyses, including histology, histomorphometry and immunohistochemistry to identify Runx2 and Osterix expression.ResultsThe density of trabecular bone, the quantity of osteoblasts and the expression of osteogenic markers, Runx2 and Osterix, were significantly higher in the LiCl group than in the control group during the orthodontic retention period.ConclusionLiCl enhances alveolar bone formation during orthodontic retention in rats.
Experienced stigma and its impacts in psychosis: The role of social rank and external shame
Authors: Wood Lisa , Irons Chris 
Published: July 2017

ObjectivesExperienced stigma is detrimental to those who experience psychosis and can cause emotional distress and hinder recovery. This study aimed to explore the relationship between experienced stigma with emotional distress and recovery in people with psychosis. It explored the role of external shame and social rank as mediators in these relationships.DesignA cross‐sectional design was implemented.MethodsFifty‐two service users were administered a battery of questionnaires examining experienced stigma, external shame, social rank, personal recovery, positive symptoms, depression, and anxiety. Correlation and multiple regression analysis were conducted on the data. Where appropriate, mediation analysis was employed to explore social rank and external shame as mediatory variables.ResultsExperienced stigma was significantly related to shame (social rank and external shame), positive symptoms, emotional distress (depression and anxiety), and personal recovery. The impact of experienced stigma on depression was mediated by external shame. Social rank was a mediator between experienced stigma and personal recovery only.ConclusionPeople with psychosis who have experienced stigma are likely to experience emotional distress and be inhibited in their recovery. This was found to be partly mediated by external shame and low social rank. Clinical approaches to stigma need to target these as potential maintenance factors.Practitioner points

Experienced stigma is significantly related to shame (social rank and external shame) emotional distress, and reduced personal recovery.

External shame mediated the relationship between experienced stigma and depression in psychosis. Social rank mediated the relationship between experienced stigma and personal recovery.

Clinical approaches to stigma should include the assessment of external shame and low social rank.
Medication adherence among adolescent solid‐organ transplant recipients: A survey of healthcare providers
Authors: Mehta Pooja , Steinberg Elizabeth A. , Kelly Sarah L. , Buchanan Cindy , Rawlinson Alana Resmini 
Published: July 2017

AbstractThe aim of this study was to assess healthcare provider perspectives on barriers to medication adherence and to discover recommendations for interventions among providers of pediatric solid‐organ transplant patients. An anonymous online survey was administered to a multidisciplinary pool of pediatric transplant providers from February 2015 to March 2016. It consisted of 15 questions regarding transplant providers’ attitudes, clinical practice, and beliefs pertaining to medication adherence among teenage solid‐organ transplant recipients. Data were analyzed using descriptive statistics. Responses to open‐ended questions were coded and categorized into themes. One hundred ten surveys were completed by providers specializing in pediatric heart, kidney, liver, lung, and/or intestinal transplantation. Commonly cited reasons for poor adherence were forgetting/poor planning (94%), the desire to be normal (86%), lack of support (86%), and poor parental monitoring (79%). Suggestions to improve adherence included increasing peer and family support, providing education, and incorporating technology into adherence regimens. Barriers to adherence in transplant patients are recognized by providers and are both similar to and disparate from patient and family identified barriers published in the literature. Providers recognize the importance of education, social support, and technologically driven interventions on improving outcomes in the transplant population.
Vindicating the Absent Qualia Objection
Authors: Conee Earl 
Published: July 2017

AbstractMetaphysical functionalism holds that the nature of the mental is its functional role. Proponents of the absent qualia objection to functionalism assert that mental states with essential phenomenal qualities might have had functional duplicates without qualia. Michael Tye has argued that this purported possibility is incoherent. Robert van Gulick has criticized Tye's argument. It is contended here that although van Gulick's criticism does not refute the argument, Tye's argument is unsuccessful. It is also contended that our evidence very strongly supports the possibility of absent qualia.
Alternate bar dynamics in response to increases and decreases of sediment supply
Authors: Bankert Andrew R. , Nelson Peter A. 
Published: July 2017

AbstractGravel‐bed rivers can accommodate changes in sediment supply by adjusting their bed topography and grain size in both the downstream and cross‐stream directions. Under high supply aggradational conditions, this can result in spatially non‐uniform stratigraphic patterns, and the morphodynamic influence of heterogeneous stratigraphy during subsequent degradational periods is poorly understood and has not been studied through physical modelling. A flume experiment was conducted to analyse channel response where alternate bars were developed in a gravel–sand mixture under constant discharge and sediment supply before two supply increases led to the development of heterogeneous stratigraphy beneath alternate bars. The supply was then reduced back to the initial supply rate, causing degradation through that self‐formed stratigraphy. Stratigraphic samples were collected, and the bed topography and flow depth were measured frequently, which were used with a two‐dimensional hydrodynamic model to characterize flow conditions. Migrating alternate bars stabilized during the first equilibrium phase, creating bed surface sorting patterns of coarse bar tops and fine pools. During the first supply increase, the bars remained stable as the pools aggraded. During the second supply increase, the pools aggraded further, causing the boundary shear stress over the bar tops to increase until the bars gained the capacity to migrate and eventually stabilize in new locations. As aggradation occurred, the original sediment sorting patterns were preserved in the subsurface. During the degradational phase, the pools experienced incision and the bars eroded laterally, but this lateral erosion ceased when coarse sediment previously deposited during the bar‐building phase became exposed. The results suggest that if a sediment supply increase is capable of filling the pools, it can cause stable bars to migrate and the bed to be reworked. These findings also show that heterogeneous stratigraphy can play an important role in determining whether bars persist or disappear after a sediment supply reduction.
Development of preferences for differently aged faces of different races
Authors: Heron‐Delaney Michelle , Quinn Paul C. , Damon Fabrice , Lee Kang , Pascalis Olivier 
Published: July 2017

AbstractChildren's experiences with differently aged faces changes in the course of development. During infancy, most faces encountered are adult, however as children mature, exposure to child faces becomes more extensive. Does this change in experience influence preference for differently aged faces? The preferences of children for adult vs. child, and adult vs. infant faces were investigated. Caucasian 3‐ to 6‐year‐olds and adults were presented with adult/child and adult/infant face pairs which were either Caucasian or Asian (race consistent within pairs). Younger children (3–4 years) preferred adults over children whereas older children (5–6 years) preferred children over adults. This preference was only detected for Caucasian faces. These data support a ‘here and now’ model of the development of face age processing from infancy to childhood. In particular, the findings suggest that growing experience with peers influences age preferences and that race impacts on these preferences. In contrast, adults preferred infants and children over adults when the faces were Caucasian or Asian, suggesting an increasing influence of a baby schema, and a decreasing influence of race. The different preferences of younger children, older children, and adults also suggest discontinuity and the possibility of different mechanisms at work during different developmental periods.
Structural, mutagenic and in silico studies of xyloglucan fucosylation in Arabidopsis thaliana suggest a water‐mediated mechanism
Authors: Urbanowicz Breeanna R. , Bharadwaj Vivek S. , Alahuhta Markus , Peña Maria J. , Lunin Vladimir V. , Bomble Yannick J. , Wang Shuo , Yang Jeong‐Yeh , Tuomivaara Sami T. , Himmel Michael E. , Moremen Kelley W. , York William S. , Crowley Michael F. 
Published: July 2017

SummaryThe mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely because of the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2‐fucosyl residues to xyloglucan side chains – a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X‐ray crystallography, mutagenesis experiments and molecular simulations. Mammalian cell culture expressions enable the sufficient production of the enzyme for X‐ray crystallography, which reveals the structural architecture of AtFUT1 in complex with bound donor and acceptor substrate analogs. The lack of an appropriately positioned active site residue as a catalytic base leads us to propose an atypical water‐mediated fucosylation mechanism facilitated by an H‐bonded network, which is corroborated by mutagenesis experiments as well as detailed atomistic simulations.
Production of low‐Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR‐Cas system
Authors: Nieves‐Cordones Manuel , Mohamed Sonia , Tanoi Keitaro , Kobayashi Natsuko I. , Takagi Keiko , Vernet Aurore , Guiderdoni Emmanuel , Périn Christophe , Sentenac Hervé , Véry Anne‐Aliénor 
Published: July 2017

SummaryThe occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side‐effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+‐permeable K+ transporter OsHAK1 with the CRISPR‐Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents.
Inactivation of Zika virus in platelet components using amotosalen and ultraviolet A illumination
Authors: Santa Maria Felicia , Laughhunn Andrew , Lanteri Marion C. , Aubry Maite , Musso Didier , Stassinopoulos Adonis 
Published: July 2017

BACKGROUNDConcerned over the risk of Zika virus (ZIKV) transfusion transmission, public health agencies recommended the implementation of mitigation strategies for its prevention. Those strategies included the use of pathogen inactivation for the treatment of plasma and platelets. The efficacy of amotosalen/ultraviolet A to inactivate ZIKV in plasma had been previously demonstrated, and the efficacy of inactivation in platelets with the same technology was assumed. These studies quantify ZIKV inactivation in platelet components using amotosalen/ultraviolet A.STUDY DESIGN AND METHODSPlatelet components were spiked with ZIKV, and ZIKV infectious titers and RNA loads were measured by cell culture‐based assays and real‐time polymerase chain reaction in spiked platelet components before and after photochemical treatment using amotosalen/ultraviolet A.RESULTSThe mean ZIKV infectivity titers and RNA loads in platelet components before inactivation were either 4.9 log10 plaque forming units per milliliter, or 4.4 log10 50% tissue culture infective dose per milliliter and 7.5 log10 genome equivalents per milliliter, respectively. No infectivity was detected immediately after amotosalen/ultraviolet A treatment. No replicative virus remained after treatment, as demonstrated by multiple passages on Vero cell cultures; and ZIKV RNA was not detected from the first passage after inactivation. Additional experiments in this study demonstrated efficient inactivation to the limit of detection in platelets manufactured in 65% platelet additive solution, 35% plasma, or 100% plasma.CONCLUSIONAs previously demonstrated for plasma, robust levels of ZIKV inactivation were achieved in platelet components. With inactivation of higher levels of ZIKV than those reported in asymptomatic, RNA‐reactive blood donors, the pathogen‐inactivation system using amotosalen/ultraviolet A offers the potential to mitigate the risk of ZIKV transmission by plasma and platelet transfusion.
A de novo germline mutation of DLX3 in a Brown Swiss calf with tricho‐dento‐osseus‐like syndrome
Authors: Hofstetter Sonja , Welle Monika , Gorgas Daniela , Balmer Pierre , Roosje Petra , Mock Thomas , Meylan Mireille , Jagannathan Vidhya , Drögemüller Cord 
Published: July 2017

ObjectiveA novel congenital disorder affecting a calf was observed, and its phenotype and genetic mutation identified.AnimalA six‐month‐old female Brown Swiss calf.MethodsDiagnostic investigation and whole genome sequencing of a case parent trio was performed.ResultsThe calf had a dull kinky coat with mild hypotrichosis, and teeth with brown staining and enamel defects. Histological examination of skin biopsies was compatible with a follicular dysplasia. Radiography and computed tomography revealed thickening of the skull bones and large pulp cavities with a marked thinning of enamel affecting all teeth. A de novo germline mutation affecting the distal‐less homeobox gene (DLX3) was identified. The 10 bp frameshift mutation in exon 3 of the bovine DLX3 gene is predicted to replace the second C‐terminal transactivation domain of the wild‐type protein by a recoded peptide of 99 amino acids without any sequence similarity.Conclusion and clinical importanceA causative mutation for a sporadic phenotype resembling human tricho‐dento‐osseous syndrome was identified after detection of a de novo germline mutation in the DLX3 gene.
Serological evidence of hepatitis E virus infection in pigs and jaundice among pig handlers in Bangladesh
Authors: Haider N. , Khan M. S. U. , Hossain M. B. , Sazzad H. M. S. , Rahman M. Z. , Ahmed F. , Zeidner N. S. 
Published: July 2017

SummaryHepatitis E virus (HEV) is the most common cause of viral hepatitis in humans. Pigs may act as a reservoir of HEV, and pig handlers were frequently identified with a higher prevalence of antibodies to HEV. The objectives of this study were to identify evidence of HEV infection in pigs and compare the history of jaundice between pig handlers and people not exposed to pigs and pork. Blood and faecal samples were collected from 100 pigs derived from three slaughterhouses in the Gazipur district of Bangladesh from January to June, 2011. We also interviewed 200 pig handlers and 250 non‐exposed people who did not eat pork or handled pigs in the past 2 years. We tested the pig sera for HEV‐specific antibodies using a competitive ELISA and pig faecal samples for HEV RNA using real‐time RT‐PCR. Of 100 pig sera, 82% (n = 82) had detectable antibody against HEV. Of the 200 pig handlers, 28% (56/200) demonstrated jaundice within the past 2 years, whereas only 17% (43/250) of controls had a history of jaundice (p < .05). Compared to non‐exposed people, those who slaughtered pigs (31% versus 15%, p < .001), reared pigs (37% versus 20%, p < .001), butchered pigs (35% versus 19%, p < .001) or involved in pork transportation (28% versus 13%, p < .001) were more likely to be affected with jaundice in the preceding 2 years. In multivariate logistic regression analysis, exposure to pigs (odds ratio [OR]: 2.2, 95% CI: 1.2–3.9) and age (OR: 0.97, 95% CI: 0.95–0.99) was significantly associated with jaundice in the past 2 years. Pigs in Bangladesh demonstrated evidence of HEV infection, and a history of jaundice was significantly more frequent in pig handlers. Identifying and genotyping HEV in pigs and pig handlers may provide further evidence of the pig's role in zoonotic HEV transmission in Bangladesh.