bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS)‐based quantification of hydroxyanthracene derivatives in Aloe vera (L.) Burm. f. gel commercial beverages and preliminary safety evaluation through in vitro genotoxicity studies
Authors: Di Minno Alessandro , Morone Maria Vittoria , Ullah Hammad , Sommella Eduardo , Buccato Daniele Giuseppe , De Lellis Lorenza Francesca , Campiglia Pietro , De Filippis Anna , Galdiero Massimiliano , Daglia Maria 
Published: September 2024

AbstractEstimating the hydroxyanthracene derivative (HAD) concentrations and assessing the genotoxicity of several marketed Aloe vera (L.) Burm. f. gel beverages are the goals of this study. The results showed that five commercial samples contained aloin A at concentrations ranging from 6.05 to 337.98 ng/mL and aloin B at concentrations ranging from 8.84 to 346.89 ng/mL. Four commercial samples contained aloin A and B in concentration higher than 1 ppm, while one sample contained less than 1 ppm. Aloe‐emodin was detected in three samples with concentrations ranging from 80.30 to 109.40 ng/mL. Different strains of bacteria were used to perform the Ames test on the samples with the lowest and highest HAD concentrations, and no signs of mutagenicity were found in experiments with samples that increased in concentration (0.0016–5 μL/mL). In accordance with published data and in contrast to findings acquired using products containing A. vera latex, which is rich in HADs and whose genotoxicity is extensively proven, even the sample with the highest concentrations of aloin A and B does not show any in vitro genotoxicity, suggesting the importance to standardize the operating procedures to obtain A. vera gel to minimize the content of HADs in the commercial formulations.
Anatomo‐functional changes in neural substrates of cognitive memory in developmental amnesia: Insights from automated and manual Magnetic Resonance Imaging examinations
Authors: Chareyron Loïc J. , Chong W. K. Kling , Banks Tina , Burgess Neil , Saunders Richard C. , Vargha‐Khadem Faraneh 
Published: September 2024

AbstractDespite bilateral hippocampal damage dating to the perinatal or early childhood period and severely impaired episodic memory, patients with developmental amnesia continue to exhibit well‐developed semantic memory across the developmental trajectory. Detailed information on the extent and focality of brain damage in these patients is needed to hypothesize about the neural substrate that supports their remarkable capacity for encoding and retrieval of semantic memory. In particular, we need to assess whether the residual hippocampal tissue is involved in this preservation, or whether the surrounding cortical areas reorganize to rescue aspects of these critical cognitive memory processes after early injury. We used voxel‐based morphometry (VBM) analysis, automatic (FreeSurfer) and manual segmentation to characterize structural changes in the brain of an exceptionally large cohort of 23 patients with developmental amnesia in comparison with 32 control subjects. Both the VBM and the FreeSurfer analyses revealed severe structural alterations in the hippocampus and thalamus of patients with developmental amnesia. Milder damage was found in the amygdala, caudate, and parahippocampal gyrus. Manual segmentation demonstrated differences in the degree of atrophy of the hippocampal subregions in patients. The level of atrophy in CA‐DG subregions and subicular complex was more than 40%, while the atrophy of the uncus was moderate (−24%). Anatomo‐functional correlations were observed between the volumes of residual hippocampal subregions in patients and selective aspects of their cognitive performance, viz, intelligence, working memory, and verbal and visuospatial recall. Our findings suggest that in patients with developmental amnesia, cognitive processing is compromised as a function of the extent of atrophy in hippocampal subregions. More severe hippocampal damage may be more likely to promote structural and/or functional reorganization in areas connected to the hippocampus. In this hypothesis, different levels of hippocampal function may be rescued following this variable reorganization. Our findings document not only the extent, but also the limits of circuit reorganization occurring in the young brain after early bilateral hippocampal damage.
Bruton tyrosine kinase inhibitor monotherapy in B‐cell lymphoma and risk of infection: A systematic review and meta‐analysis of randomized controlled trials
Authors: Zuber Mohammed , Borate Samruddhi Nandkumar , Gokhale Pooja , Yerubandi Akhila , Alam Bhuiya N. M. Mahmudul , Rawal Smita , Young Henry N. , Villa Zapata Lorenzo 
Published: September 2024

AbstractBruton's tyrosine kinase (BTK) inhibitors are important therapeutic advances with promising efficacy outcomes in the treatment of patients with chronic lymphocytic leukemia and other B‐cell lymphoma subtypes. However, the utility of BTK inhibitors can be limited by adverse events such as infections. In this systematic review and meta‐analysis, we aim to determine the risk of various infections associated with BTK inhibitor monotherapy in B‐cell lymphoma patients. A comprehensive search was conducted in MEDLINE/PubMed, Embase, and Web of Science databases from their inception until October 2023. ClinicalTrials.gov, bibliographies, and relevant conference abstracts were also searched for additional records. Randomized controlled trials that included any B‐cell lymphoma patients treated with BTK inhibitor monotherapy and reported infection were included. Meta‐analysis was performed to calculate risk ratio (RR) using a random‐effects model in R Statistical Software, version 4.3.2. Of 3292 studies retrieved, we included 12 studies in this systematic review and meta‐analysis. The median age of patients across the study arms ranged between 64 and 73 years. The overall pooled RR for any grade upper respiratory tract infections (URTI) associated with BTK inhibitor treatment was 1.55 (95% Confidence Interval (CI) 1.22–1.97). The RR of grade ≥3 URTI was reported in 14 out of 1046 patients, yielding an RR of 1.46 (95% CI 0.61–3.54), which was not statistically significant. The pooled RR of any grade pneumonia was 1.20 (95% CI 0.68–2.10) and grade ≥3 pneumonia was 1.12 (95% CI 0.67–1.85), both of which were not statistically significant. Patients with B‐cell lymphoma who are undergoing BTK inhibitor monotherapy face an elevated risk of developing URTI. Clinicians prescribing BTK inhibitors should be aware of the potential infectious events that may occur. Close monitoring and the implementation of effective prophylactic measures are essential for managing these patients.
Review of triage strategies for atypical squamous cells of undetermined significance among young women
Authors: Cubaka Ntamushigo Jeremie , Motshedisi Sebitloane Hannah 
Published: September 2024

AbstractIn the present study we reviewed the existing literature regarding management approaches for ASC‐US and highlight their pros and cons. The ASC‐US entity emerged from Bethesda classification 2001. We conducted this review using search words ASC‐US triage, ASC‐US management in young women, triage tests for ASC‐US, and ASC‐US outcome from the English literature. We included different cervical cancer policies (American, European and for WHO) and research articles published on ASC‐US in young women from the year 2001. We searched in Google Scholar, PubMed, MEDLINE (NCBI) library, Embase (Elsevier), Wiley online library as well as Cochrane library. We defined young women as aged 30 years and below. We identified 52 articles which focused on management approaches of ASC‐US, seven articles focused on young women aged <30 years. Five of these articles combined ASC‐US with low‐grade squamous intraepithelial lesions (ASC‐US/LSIL) while only two addressed ASC‐US as a standalone entity. The limited number of articles restricts the evidence base supporting the adoption of triage strategies. There is yet, no consensus in the literature regarding the management of ASC‐US, more so in young women below the age of 30 years. Researchers, however, agree on a few aspects, which include the necessity for applying a conservative strategy for managing ASC‐US in young women, avoiding direct referral for colposcopy at the initial detection of ASC‐US, and avoiding the use of human papillomavirus (HPV) testing on young women (unless living with HIV). Newer techniques such as HPV E6/E7 messenger RNA (mRNA), and dual staining p16/ki‐67, may serve as better triage to identify cases of HPV persistence and integration which may subsequently lead to preinvasive or invasive lesions.
PLXDC1 serves as a potential prognostic marker and involves in malignant progression and macrophage polarization in colon cancer
Authors: Fu Yu , Zhang Xuan , Qiao Qing 
Published: September 2024

AbstractThe malignant behavior and immune escape ability of cancer cells lead to therapeutic failure and poor prognosis for patients with various cancers, including colon cancer. Plexin domain containing 1 (PLXDC1) was initially identified to exert key roles in tumor by regulating angiogenesis and has recently proved to be involved in cell proliferation and migration of glioblastoma and gastric cancer cells. However, its roles in colon cancer remain unclear. In this study, the online bioinformatics databases confirmed high expression of PLXDC1 in colon cancer specimens, which was associated with cancer stages and nodal metastasis. Similarly, the increased expression of PLXDC1 was also validated in our collected samples and colon cancer cells. Moreover, patients with high expression of PLXDC1 had shorter survival, indicating that PLXDC1 might be a potential prognostic predictor for colon cancer patients. Notably, targeting PLXDC1 inhibited cancer cell viability and invasion, and enhanced cell apoptosis. Intriguingly, Tumor Immune Estimation Resource database confirmed that PLXDC1 expression was related to various tumor‐infiltrating immune cells in colon adenocarcinoma including macrophages, and its expression was also correlated with M2‐like macrophage markers. In vitro, colon cancer cells with PLXDC1 downregulation had a reduced ability to recruit and polarize macrophage towards M2 phenotype by decreasing the percentage of CD206+ cells and M2‐like markers (CD206, CD163, arginase1, and interleukin 10 [IL‐10]). Moreover, PLXDC1 knockdown attenuated M2 macrophage‐mediated promotion in cancer cell viability and invasion. Mechanically, inhibition of PLXDC1 suppressed activation of the IL‐6/Signal transducer and activator of transcription 3 (STAT3) signaling. Reactivating the above pathway by transfection with IL‐6 plasmids reversed the suppressive effects of PLXDC1 knockdown on cancer cell malignant behaviors, macrophage recruitment and M2‐like polarization. Thus, PLXDC1 downregulation may inhibit the malignancy of colon cancer cells and their ability to recruit and polarize macrophages towards M2 phenotype by blocking the IL‐6/STAT3 pathway. Together, targeting PLXDC1 may attenuate the progression of colon cancer by direct roles in cancer cells and indirect roles in macrophage polarization, representing a promising therapeutic target for colon cancer patients.
Evaluating the Association of Anthropometric Indices With Total Cholesterol in a Large Population Using Data Mining Algorithms
Authors: Yousefabadi Sahar Arab , Ghiasi Hafezi Somayeh , Kooshki Alireza , Hosseini Marzieh , Mansoori Amin , Ghamsary Mark , Esmaily Habibollah , Ghayour‐Mobarhan Majid 
Published: September 2024

ABSTRACTBackgroundUnbalanced levels of serum total cholesterol (TC) and its subgroups are called dyslipidemia. Several anthropometric indices have been developed to provide a more accurate assessment of body shape and the health risks associated with obesity. In this study, we used the random forest model (RF), decision tree (DT), and logistic regression (LR) to predict total cholesterol based on new anthropometric indices in a sex‐stratified analysis.MethodOur sample size was 9639 people in which anthropometric parameters were measured for the participants and data regarding the demographic and laboratory data were obtained. Aiding the machine learning, DT, LR, and RF were drawn to build a measurement prediction model.ResultsAnthropometric and other related variables were compared between both TC <200 and TC ≥200 groups. In both males and females, Lipid Accumulation Product (LAP) had the greatest effect on the risk of TC increase. According to results of the RF model, LAP and Visceral Adiposity Index (VAI) were significant variables for men. VAI also had a stronger correlation with HDL‐C and triglyceride. We identified specific anthropometric thresholds based on DT analysis that could be used to classify individuals at high or low risk of elevated TC levels. The RF model determined that the most important variables for both genders were VAI and LAP.ConclusionWe tend to present a picture of the Persian population's anthropometric factors and their association with TC level and possible risk factors. Various anthropometric indices indicated different predictive power for TC levels in the Persian population.
Refining and expanding applications of Moral Foundations Theory in consumer psychology
Authors: Johnson Wayne , Ramos Guilherme A. , VanEpps Eric M. , Graham Jesse 
Published: September 2024

AbstractWe set forth an agenda for Moral Foundations Theory (MFT) research in consumer psychology, focusing in particular on four pathways: (1) factoring in multiple identities, including moral identities, to account for contextual elevation or suppression of moral foundations in predicting which decisions consumers moralize and when; (2) broadening the methodological usage of MFT to include more targeted causal research as well as expanding the utility of correlational research; (3) increasing discriminant validity between MFT and other constructs by studying moral foundations as individually manipulable and focusing on their incremental predictive validity over and above demographics and related constructs; and (4) recognizing that researcher biases regarding morality can leak into the publication process, necessitating clear distinctions between prescriptive versus descriptive research. These pathways facilitate more precise and stronger predictive validity for applying MFT in consumer psychology, yielding greater theoretical and practical utility across researcher perspectives.
Dynamics of microRNA secreted via extracellular vesicles during the maturation of embryonic stem cell‐derived retinal pigment epithelium
Authors: Pollalis Dimitrios , Nair Gopa Kumar Gopinadhan , Leung Justin , Bloemhof Clarisa Marie , Bailey Jeffrey K. , Pennington Britney O. , Kelly Kaitlin R. , Khan Amir I. , Yeh Ashley K. , Sundaram Kartik S. , Clegg Dennis O. , Peng Chen‐Ching , Xu Liya , Georgescu Constantin , Wren Jonathan D. , Lee Sun Young 
Published: September 2024

AbstractRetinal pigment epithelial (RPE) cells are exclusive to the retina, critically multifunctional in maintaining the visual functions and health of photoreceptors and the retina. Despite their vital functions throughout lifetime, RPE cells lack regenerative capacity, rendering them vulnerable which can lead to degenerative retinal diseases. With advancements in stem cell technology enabling the differentiation of functional cells from pluripotent stem cells and leveraging the robust autocrine and paracrine functions of RPE cells, extracellular vesicles (EVs) secreted by RPE cells hold significant therapeutic potential in supplementing RPE cell activity. While previous research has primarily focused on the trophic factors secreted by RPE cells, there is a lack of studies investigating miRNA, which serves as a master regulator of gene expression. Profiling and defining the functional role of miRNA contained within RPE‐secreted EVs is critical as it constitutes a necessary step in identifying the optimal phenotype of the EV‐secreting cell and understanding the biological cargo of EVs to develop EV‐based therapeutics. In this study, we present a comprehensive profile of miRNA in small extracellular vesicles (sEVs) secreted during RPE maturation following differentiation from human embryonic stem cells (hESCs); early‐stage hESC‐RPE (20–21 days in culture), mid‐stage hESC‐RPE (30–31 days in culture) and late‐stage hESC‐RPE (60–61 days in culture). This exploration is essential for ongoing efforts to develop and optimize EV‐based intraocular therapeutics utilizing RPE‐secreted EVs, which may significantly impact the function of dysfunctional RPE cells in retinal diseases.
Differential regulation of physicochemical properties and myofibrillar protein degradation of yak meat by interactions between reactive oxygen species and reactive nitrogen species during postmortem aging
Authors: Yang Xue , Bu Xinrong , Li Yiheng , Shen Ruheng , Duan Yufeng , Shi Hongmei , Kong Xiangying , Zhang Li 
Published: September 2024

AbstractBACKGROUNDThis study aimed to explore how interactions between reactive oxygen species (ROS) and reactive nitrogen species (RNS) affect oxidative properties, nitrosative properties, and myofibrillar protein degradation during postmortem aging of yak meat.RESULTSYak longissimus dorsi was incubated with saline, ROS activator (H2O2)/inhibitor N‐Acetyl‐L‐cysteine (NAC) and RNS activator S‐Nitrosoglutathione (GSNO)/inhibitor L‐NAME hydrochloride (L‐NAME) combined treatments at 4 °C for 12, 24, 72, 120, and 168 h. The results indicated that regardless of whether RNS was activated or inhibited, activated ROS played a dominant role in myofibrillar protein degradation by oxidative modification to increase carbonyl content, disulfide bonds, surface hydrophobicity, and dimerized tyrosine while decreasing sulfhydryl content, thereby degrading nebulin, titin, troponin‐t and desmin. Notably, the Warner–Bratzler shear force (WBSF) of the H2O2 + L‐NAME group was the smallest, whereas that of the NAC + GSNO group was smaller than that of the NAC + L‐NAME group.CONCLUSIONThese findings provide new insights into meat tenderization patterns through the interaction between ROS and RNS. © 2024 Society of Chemical Industry.
Correlation analysis between Radix Pseudostelariae quality and rhizosphere soil factors of Pseudostellaria heterophylla in a cultivation region, China
Authors: Zheng Jiao , Yang Xin , An Na , Yang Dan 
Published: September 2024

AbstractBACKGROUNDPseudostellaria heterophylla, known for its significant bioactive ingredients, offers potential health benefits. Amounts of bioactive compounds of the tuberous root of cultivated Pseudostellaria heterophylla are sensitive to environmental conditions. We selected 22 sampling sites in Guizhou Province, China, a primary Pseudostellaria heterophylla planting area. We analyzed polysaccharides, water‐soluble extractives, total ash and inorganic elements (Fe, Mn, Zn, Mg and Ca) in Radix Pseudostellariae, and pH, organic carbon (OC), available nitrogen (AN), available phosphorus (AP), available potassium (AK) and inorganic elements in the soil.RESULTSOur study revealed a substantial presence of polysaccharides (85.00–181.00 mg g−1), water‐soluble extractives (47.52–57.63%) and total ash (1.87–3.39%) in Radix Pseudostellariae. Polysaccharides and total ash showed no sensitivity to soil pH. Radix Pseudostellariae collected from soil with pH > 7 exhibited slightly higher levels of water‐soluble extractives, Mg and Ca than that from soil with pH < 5. Conversely, soil with a pH less than 5 had higher OC, AN, AP and AK contents. Water‐soluble extractives in Radix Pseudostellariae were negatively correlated with soil pH but positively correlated with OC and AN.CONCLUSIONThe results imply that the sequestration of soil nutrients over long‐term Pseudostellaria heterophylla cultivation could negatively impact the accumulation of some bioactive ingredients in Radix Pseudostellariae. This study has a profound implication for enhancing the quality of Radix Pseudostellariae of artificially cultivated Pseudostellaria heterophylla. © 2024 Society of Chemical Industry.
Improved encapsulation efficiency and storage stability of lutein by soy protein isolate nanocarriers with thermal and trypsin treatments
Authors: Cheng Yong , Wu Renyi , Xiao Dong , Wang Zhaojun , Chen Qiuming , Zeng Maomao , Qin Fang , Chen Jie , He Zhiyong 
Published: September 2024

AbstractBACKGROUNDEncapsulation of bioactive compounds within protein‐based nanoparticles has garnered considerable attention in the food and pharmaceutical industries because of its potential to enhance stability and delivery. Soy protein isolate (SPI) has emerged as a promising candidate, prompting the present study aiming to modify its properties through controlled thermal and trypsin treatments for improved encapsulation efficiency (EE) of lutein and its storage stability.RESULTSThe EE of lutein nanoparticles encapsulated using SPI trypsin hydrolysates (SPIT) with three varying degrees of hydrolysis (4.11%, 6.91% and 10.61% for SPIT1, SPIT2 and SPIT3, respectively) increased by 12.00%, 15.78% and 18.59%, respectively, compared to SPI. Additionally, the photostability of SPIT2 showed a remarkable increase of 38.21% compared to SPI. The superior encapsulation efficiency and photostability of SPIT2 was attributed to increased exposure of hydrophobic groups, excellent antioxidant activity and uniform particle stability, despite exhibiting lower binding affinity to lutein compared to SPI. Furthermore, in SPIT2, the protein structure unfolded, with minimal impact on overall secondary structure upon lutein addition.CONCLUSIONThe precise application of controlled thermal and trypsin treatments to SPI has been shown to effectively produce protein nanoparticles with substantially improved encapsulation efficiency for lutein and enhanced storage stability of the encapsulated lutein. These findings underscore the potential of controlled thermal and trypsin treatments to modify protein properties effectively and offer significant opportunities for expanding the applications of protein‐based formulations across diverse fields. © 2024 Society of Chemical Industry.
Additives improve the fermentation quality, anthocyanin content, and biological activity of purple Napier grass silage
Authors: Yin Xiang , Wu Juanyan , Shen Lan , He Qianqian , He Shuzhen , Yuan Muhui , Sun Jianxia , Zhang Jianguo 
Published: September 2024

AbstractBACKGROUNDPurple Napier grass (PNG), a widely used grass rich in anthocyanin, is commonly employed in the production of silage. However, there is currently limited research on the retention of anthocyanin with or without additives during ensiling. Therefore, this study aimed to investigate the effect of different additives (Lactiplantibacillus plantarum CCZZ1 (LP), glucose, acetic acid, and dried soybean curd residue) on fermentation quality, anthocyanin content, and microbial community structure of PNG silage.RESULTSEnsiling PNG without additives led to poor fermentation quality and rapid degradation of anthocyanin, resulting in a decline in antioxidant activity and the persistence of harmful microorganisms with high relative abundance. The use of additives, especially LP, effectively increased the relative abundance of L. plantarum, enhancing fermentation quality, the retention of anthocyanin (up to 166% increase rate) and antioxidant activity, while reducing the relative abundance of harmful microorganisms during ensiling for 30 days. Additionally, prolonged ensiling negatively affected the preservation of anthocyanin. Based on both fermentation quality and bioactivity, PNG should be ensiled for 30 days with LP inoculation.CONCLUSIONThe employment of additives, especially LP, improved the fermentation quality, anthocyanin retention, and microbial community structure in PNG silage. To optimize both fermentation quality and bioactivity, it is recommended that PNG be ensiled for 30 days with LP inoculation. © 2024 Society of Chemical Industry.