bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Therapeutic Effects of Lycopene Against Experimental Giardiasis in Mice
Authors: Parvaneh Jahan , Namazi Fatemeh , Razavi Seyed Mostafa , Nazifi Saeed , Nayebzadeh Hassan 
Published: October 2024

ABSTRACTGiardia duodenalis is a protozoan parasite that infects approximately one billion people worldwide. In this study, the effects of lycopene on experimental giardiasis in mice were investigated by evaluating cyst shedding rate, weight changes, duodenal antioxidant status, and histopathological alteration. Ninety‐five male mice aged four to six weeks were divided into six groups of 15 and one group of 5 as the negative control. All mice were infected with 2 × 105 Giardia duodenalis (B genotype) cysts except for the negative control. The treatment groups were treated with metronidazole, mixed lycopene‐metronidazole, and 5, 10, and 20 mg/kg lycopene for 7 days. The mice treated with lycopene 20 and mixed lycopene‐metronidazole had no cyst shedding from the 7th day of treatment. The mildest lesions were observed in the mixed lycopene‐metronidazole group. Results showed that treatment with mixed lycopene‐metronidazole significantly increased TAC and decreased MDA levels as compared with the positive control. It seems that the antigiardial effect of lycopene is related to its antioxidant properties. Nevertheless, direct effect of lycopene on Giardia and its probable mechanism remain to be investigated.
Gastroprotective Efficacy of North African Medicinal Plants: A Review on Their Therapeutic Potential for Peptic Ulcers
Authors: Cherrada Nezar , Chemsa Ahmed Elkhalifa , Gheraissa Noura , Laib Ibtissam , Gueboudji Zakia , EL‐Shazly Mohamed , Zaater Abdelmalek , Abid Asma , Sweilam Sherouk Hussein , Emran Talha Bin , Nani Sadok , Benamor Bilal , Ghemam Amara Djilani , Atoki Ayomide Victor , Messaoudi Mohammed 
Published: October 2024

ABSTRACTPeptic ulcer disease remains a prevalent gastrointestinal disorder worldwide. Current treatments often have limitations, sparking interest in alternative therapies from medicinal plants. This review examines the gastroprotective potential of 54 North African medicinal plants against peptic ulcers. An extensive literature search was conducted, focusing on plants with preclinical and clinical evidence of anti‐ulcer efficacy and documented use in North African traditional medicine. The review identified several promising plant species, such as licorice (Glycyrrhiza glabra), chamomile (Matricaria chamomilla), olive (Olea europaea), pomegranate (Punica granatum), Aloe vera, and black seed (Nigella sativa), along with their bioactive constituents, including flavonoids, tannins, and terpenoids. These compounds exhibit gastroprotective properties through multiple mechanisms, such as enhancing the gastric mucosal barrier, inhibiting acid secretion, displaying antioxidant and anti‐inflammatory effects, promoting ulcer healing, and combating Helicobacter pylori infection. The evidence presented includes in vitro assays, animal models, and some clinical studies. While many of the 53 plants reviewed demonstrated significant anti‐ulcer effects compared to standard drugs, further clinical research is needed to establish efficacy and safety in humans. The synergistic actions of phytochemical mixtures in medicinal plant extracts likely contribute to their therapeutic potential. This review highlights the role these North African medicinal plants may play in the prevention and treatment of peptic ulcers and identifies promising candidates for further research and development of evidence‐based botanical therapies.
Late Ordovician Bentonites From the Southern Ordos Basin: Response to the Subduction of the Proto‐Tethys Ocean
Authors: Li Zuochen , Pei Xianzhi , Pei Lei , Chen Youxin , Wang Hao , Lin Hao , Qin Li , Yang Yajie , Ji Shang , Chen Liang'e , Hou Weiyi , Hussain Irshad , Zhang Gang 
Published: October 2024

ABSTRACTThe connection between the Ordovician bentonites on the southern margin of the Ordos Basin and the Early Palaeozoic volcanic rocks of the North Qinling Orogenic Belt is crucial for understanding the subduction and collisional closure of the Shangdan Ocean during the Early Palaeozoic. This paper investigates zircon U–Pb ages, geochemistry and Lu–Hf isotopic compositions of zircons in the Upper Ordovician Zhaolaoyu Formation bentonites located on the southern margin of the Ordos Basin. U–Pb dating of zircon indicates a coeval age of 453.3 ± 1.4 Ma (MSWD = 0.99), which represents the crystallisation age during the Late Ordovician Katian stage. The bentonites exhibit higher SiO2 (57.94–77.95 wt.%) and Al2O3 (9.21–14.33 wt.%), classifying them within the low‐potassium alkali basalt to medium‐potassium calc‐alkaline series. The parent rock of the bentonites is likely intermediate to felsic volcanic rocks. The rare earth element partitioning curves of the bentonites are right‐dipping, with a more pronounced negative Eu anomaly (δEu = 0.48–0.67). The zircons in the bentonites yield two‐stage model ages ranging from 546 to 956 Ma, along with ε
Hf(t) values between 5.56 and 13.55. These results indicate that the bentonites are products of volcanic arc magma formed in a subduction–collision environment. The interbedded bentonites in the Upper Ordovician limestones of the southern margin of the Ordos Basin may be associated with the northward subduction of the Shangdan Oceanic crust, reflecting the subduction and consumption of the Proto‐Tethys Ocean along the southern margin of the North China Block.
Multimodal Connectivity‐Guided Glioma Segmentation From Magnetic Resonance Images via Cascaded 3D Residual U‐Net
Authors: Sun Xiaoyan , Hu Chuhan , He Wenhan , Yuan Zhenming , Zhang Jian 
Published: October 2024

ABSTRACTGlioma is a type of brain tumor with a high mortality rate. Magnetic resonance imaging (MRI) is commonly used for examination, and the accurate segmentation of tumor regions from MR images is essential to computer‐aided diagnosis. However, due to the intrinsic heterogeneity of brain glioma, precise segmentation is very challenging, especially for tumor subregions. This article proposed a two‐stage cascaded method for brain tumor segmentation that considers the hierarchical structure of the target tumor subregions. The first stage aims to identify the whole tumor (WT) from the background area; and the second stage aims to achieve fine‐grained segmentation of the subregions, including enhanced tumor (ET) region and tumor core (TC) region. Both stages apply a deep neural network structure combining modified 3D U‐Net with a residual connection scheme to tumor region and subregion segmentation. Moreover, in the training phase, the 3D masks generation of subregions with potential incomplete connectivity are guided by the completely connected regions. Experiments were performed to evaluate the performance of the methods on both area and boundary accuracy. The average dice score of the WT, TC, and ET regions on BraTS 2020 dataset is 0.9168, 0.0.8992, 0.8489, and the Hausdorff distance is 6.021, 9.203, 12.171, respectively. The proposed method outperforms current works, especially in segmenting fine‐grained tumor subregions.
Oral acute graft‐versus‐host disease
Authors: Teymouri Farzad , Sebastian Gale , Gem Hakan , Minot Samuel S. , Rashidi Armin 
Published: October 2024

AbstractOral acute graft‐versus‐host disease (aGVHD) is rare and with no diagnostic criteria. We report a case of oral aGVHD with three clinical phases. A self‐limited prodrome of largely subjective oral symptoms was followed by concurrent oral and upper gastrointestinal aGVHD. Six months after transplantation, the patient was diagnosed with severe oral and upper gastrointestinal chronic GVHD. We compared the salivary microbiota of our patient at the time of diagnosis of aGVHD with 50 contemporaneous transplant recipients and found no evidence for oral microbiota involvement in pathogenesis. This in‐depth N‐of‐1 analysis reveals novel aspects of oral aGVHD pathogenesis.
Restoring historical moose densities results in fewer wolves killed for woodland caribou conservation
Authors: McLellan Michelle L. , Ford Adam T. , Hervieux Dave , Lamb Clayton T. , Hessami Mateen , Bridger Michael C. , Serrouya Robert 
Published: October 2024

AbstractWoodland caribou (Rangifer tarandus caribou) are declining across much of their distribution in Canada in response to habitat alteration, leading to unsustainable predation, particularly by wolves (Canis lupus). Habitat alteration can benefit the primary prey species of wolves (moose [Alces alces] and deer [Odocoileus spp.]) by creating early seral conditions that contain more of their preferred food types. This increase in primary prey populations results in elevated wolf abundance and heightened predation pressure on caribou. In response to the elevated wolf populations and the risks to caribou, managers have reduced wolf abundance in key areas. Ecological theory suggests that reducing wolf abundance would release moose from the top‐down effects of wolf predation, potentially allowing moose populations to grow. Elevated moose abundance thus has the potential to cause wolf populations to rebound quickly each year following reductions, suggesting a possible link between moose abundance and the number of wolves killed for caribou conservation. To test this idea we used a unique management situation in British Columbia and Alberta, Canada, where lethal wolf removals were annually conducted across specific southern mountain caribou population ranges and, in some places, moose populations were concurrently reduced via liberalized hunting. We used indices of moose abundance and wolf removal data to test the hypothesis that reducing moose populations to a historical abundance target by hunting leads to fewer wolves killed for caribou conservation. After controlling for habitat quality, wolves removed per km2 was 3.2 times lower in areas with reduced moose density ( = 1.55 wolves/1,000 km2 ± 0.33 [SE]) than in those without reduced moose density ( = 5.02 wolves/1,000 km2 ± 0.52). However, the average number of wolves removed per year decreased under both conditions. After 9 years, there was a 35% reduction in the predicted difference in the annual removal between areas with and without moose reduction. Our results suggest that policies that do not reduce or stabilize moose abundance will result in the removal of more wolves to increase caribou abundance. Like wolf reductions, moose reductions can also be controversial and affect local harvesters. Thus, understanding the consequences of actions that support caribou recovery is essential to supporting evidence‐based policy discussions.
Tunable Blended Collagen I/II and Collagen I/III Hydrogels as Tissue Mimics
Authors: Babiak Paulina M. , Battistoni Carly M. , Cahya Leonard , Athreya Rithika , Minnich Jason , Panitch Alyssa , Liu Julie C. 
Published: October 2024

AbstractCollagen (Col) is commonly used as a natural biomaterial for biomedical applications. Although Col I is the most prevalent col type employed, many collagen types work together in vivo to confer function and biological activity. Thus, blending collagen types can better recapitulate many native environments. This work investigates how hydrogel properties can be tuned through blending collagen types (col I/II and col I/III) and by varying polymerization temperatures. Col I/II results in poorly developed fibril networks, which softened the gels, especially at lower polymerization temperatures. Conversely, col I/III hydrogels exhibit well‐connected fibril networks with localized areas of fine fibrils and result in stiffer hydrogels. A decreased molecular mass recovery rate is observed in blended hydrogels. The altered fibril morphologies, mechanical properties, and biological signals of the blended gels can be leveraged to alter cell responses and can be used as models for different tissue types (e.g., healthy vs fibrotic tissue). Furthermore, the biomimetic hydrogel properties are a tool that can be used to modulate the transport of drugs, nutrients, and wastes in tissue engineering applications.
Riboflavin‐Catalyzed Photoinduced Atom Transfer Radical Polymerization
Authors: Coskun Halil Ibrahim , Votruba‐Drzal Thomas , Wu Hanshu , Jockusch Steffen , Yilmaz Gorkem , Matyjaszewski Krzysztof 
Published: October 2024

AbstractThe photoATRP of methyl acrylate (MA) is investigated using riboflavin (RF) and CuBr2/Me6TREN as a dual catalyst system under green LED irradiation (λ ≈ 525 nm). Both RF and CuBr2/Me6TREN enhanced oxygen tolerance, enabling effective ATRP in the presence of residual oxygen. High molar mass polymers (up to Mn ≈ 129 000 g·mol−1) with low dispersity (Đ ≤ 1.16) are prepared, and chain‐end fidelity is confirmed through successful chain extension. The molecular masses of the obtained polymer increased linearly with conversion and showed high initiation efficiency. Mechanistic studies by laser flash photolysis reveal that the predominant activator generation mechanism is reductive quenching of RF by Me6TREN (83%, under [CuBr2]/[Me6TREN] = 1/3 condition), supported by polymerization kinetics and thermodynamic calculations.
Metal‐organic frameworks‐based nanomedicines to promote cancer immunotherapy: Recent advances and future directions
Authors: Feng Chenqian , Liang Xiaoyan , Fan Rangrang , Mu Min , Zhou Liangxue , Guo Gang 
Published: October 2024

AbstractCancer immunotherapy uses the body's immune system to fight tumors by restoring natural antitumor responses. Metal‐organic frameworks (MOFs), characterized by their unique crystalline porous structures formed from metal ions linked by organic ligands, offer a promising solution. Recent studies have unveiled the potential of MOFs in cancer immunotherapy. The exceptional porosity and surface area, coupled with their extraordinary thermal and chemical stability, bring significant advantages for efficient drug loading and delivery of immunotherapeutic agents. The adaptability of MOFs further enhances the controlled release of immunotherapeutic drugs within target cells and increases tumor sensitivity to other therapies such as photodynamic, photothermal, and radiotherapy. This multifunctional carrier contributes to modulating the tumor microenvironment and reactivating antitumor immunity, providing a comprehensive strategy for cancer treatment. In this review, we summarize the applications of MOFs in immune checkpoint blockade, immunomodulator delivery, and cancer vaccine delivery, and discuss existing challenges in their use for immunotherapy. This discussion aims to offer insights for developing better treatments and enhancing the efficacy of immunotherapy.
Feedback loop LINC00511–YTHDF2–SOX2 regulatory network drives cholangiocarcinoma progression and stemness
Authors: Guan Canghai , Zou Xinlei , Gao Xin , Liu Sidi , Gao Jianjun , Shi Wujiang , Dong Qingfu , Jiang Xingming , Zhong Xiangyu 
Published: October 2024

AbstractCholangiocarcinoma (CCA) was identified as a malignant tumor with rising incidence and mortality rates, and the roles of long noncoding RNA (lncRNA) in CCA remained not entirely clear. In this study, LINC00511 had high expression in CCA, which was closely related to poor prognosis. Knockdown of LINC00511 significantly inhibited cell malignant biological behaviors. It also affected the stemness of CCA, evidenced by decreased SOX2 protein expression. Moreover, the study revealed the interaction of LINC00511, YTHDF2, and SOX2 in CCA. Specifically, LINC00511 facilitated the formation of a complex with YTHDF2 on SOX2 mRNA, which uniquely enhances the mRNA's stability through m6A methylation sites. This stabilization appears crucial for maintaining malignant behaviors in CCA cells. Additionally, LINC00511 modulated SOX2 expression via the PI3K/AKT signaling pathway. Meanwhile, SOX2 can also promote LINC00511 expression as an upstream transcription factor, thereby confirming a positive feedback loop formed by LINC00511, YTHDF2, and SOX2, which plays a significant role in the occurrence and development of CCA. Finally, the study successfully constructed two patient‐derived xenograft models, revealing the vital role of LINC00511 in CCA development. In summary, this research provides a comprehensive understanding of the role of LINC00511 in the pathogenesis of CCA.
RNA modification in normal hematopoiesis and hematologic malignancies
Authors: Chen Xi , Yuan Yixiao , Zhou Fan , Li Lihua , Pu Jun , Jiang Xiulin 
Published: October 2024

AbstractN6‐methyladenosine (m6A) is the most abundant RNA modification in eukaryotic cells. Previous studies have shown that m6A plays a critical role under both normal physiological and pathological conditions. Hematopoiesis and differentiation are highly regulated processes, and recent studies on m6A mRNA methylation have revealed how this modification controls cell fate in both normal and malignant hematopoietic states. However, despite these insights, a comprehensive understanding of its complex roles between normal hematopoietic development and malignant hematopoietic diseases remains elusive. This review first provides an overview of the components and biological functions of m6A modification regulators. Additionally, it highlights the origin, differentiation process, biological characteristics, and regulatory mechanisms of hematopoietic stem cells, as well as the features, immune properties, and self‐renewal pathways of leukemia stem cells. Last, the article systematically reviews the latest research advancements on the roles and mechanisms of m6A regulatory factors in normal hematopoiesis and related malignant diseases. More importantly, this review explores how targeting m6A regulators and various signaling pathways could effectively intervene in the development of leukemia, providing new insights and potential therapeutic targets. Targeting m6A modification may hold promise for achieving more precise and effective leukemia treatments.
Tall tower observations of a northward surging gust front in central Amazon and its role in the mesoscale transport of carbon dioxide
Authors: Reis Luciane I. , Oliveira Maurício I. , Dias‐Júnior Cléo Q. , van Asperen Hella , Mortarini Luca , Acevedo Otávio C. , Pöhlker Christopher , Kremper Leslie A. , Takeshi Bruno , Quesada Carlos A. , Brondani Daiane V. 
Published: October 2024

AbstractHigh‐frequency measurements obtained at two micrometeorological towers are investigated for a rare northward surging gust front that impacted the Amazon Tall Tower Observatory (ATTO), in central Amazon. The gust front originated from a decaying mesoscale convective system (MCS) during the morning hours of 27 December 2021 near Manaus, Amazonas state, northern Brazil, and surged north‐eastward towards the ATTO site. Large temperature drops and vigorous, persistent winds were observed at the towers which lasted for over 4 h despite the gust front being detached from its parent, decaying MCS. More importantly, the gust front was responsible for drastic increases of CO2 concentrations throughout the tower depths, which suggests that the gust front winds horizontally advected CO2‐rich air from a source upstream from the ATTO site. The CO2‐rich outflow is hypothesized to originate from downward transport and/or biomass burning from forest fires in southeastern Amazon, both ideas that are supported by large increases of aerosol concentrations measured at ATTO following the gust front passage. Our results stress the need for further investigations addressing the role played by mesoscale convective circulations in the redistribution of trace gases and aerosols in the Amazon.
Malnutrition in patients with obesity: An overview perspective
Authors: Cristancho Cagney , Mogensen Kris M. , Robinson Malcolm K. 
Published: October 2024

AbstractMalnutrition in patients with obesity presents a complex and often overlooked clinical challenge. Although obesity is traditionally associated with overnutrition and excessive caloric intake, it can also coincide with varying degrees of malnutrition. The etiopathogenesis of obesity is multifaceted and may arise from several factors such as poor diet quality, nutrient deficiencies despite excess calorie consumption, genetics, and metabolic abnormalities affecting nutrient absorption and utilization. Moreover, a chronic low‐grade inflammatory state resulting from excess adipose tissue, commonly observed in obesity, can further exacerbate malnutrition by altering nutrient metabolism and increasing metabolic demands. The dual burden of obesity and malnutrition poses significant risks, including immune dysfunction, delayed wound healing, anemia, metabolic disturbances, and deficiencies in micronutrients such as vitamin D, iron, magnesium, and zinc, among others. Malnutrition is often neglected or not given enough attention in individuals with obesity undergoing rapid weight loss through aggressive caloric restriction, pharmacological therapies, and/or surgical interventions. These factors often exacerbate vulnerability to nutrition deficiencies. We advocate for healthcare practitioners to prioritize nutrition assessment and initiate medical intervention strategies tailored to address both excessive caloric intake and insufficient consumption of essential nutrients. Raising awareness among healthcare professionals and the general population about the critical role of adequate nutrition in caring for patients with obesity is vital for mitigating the adverse health effects associated with malnutrition in this population.
Optimization of Electrodialysis for Ammonium Removal From NH4Cl‐Doped Groundwater Samples Using the Response Surface Method
Authors: Hazra Mohamed , Addar Fatima Zahra , Tahaikt Mustapha , Elmidaoui Azzedine , Taky Mohamed , Belhamidi Sakina 
Published: October 2024

AbstractThis study aims to optimize ammonium removal from NH4Cl‐enriched groundwater at different concentrations using an electrodialysis (ED) process. A customized design (CD) based on response surface methodology (RSM) was employed to develop predictive models and improve the performance of the demineralization system. Ion removal efficiency was evaluated in 32 unique experimental configurations, taking into account variations in three input parameters: voltage (A), initial ammonium concentration (B) and demineralization rate (C). These parameters were selected for their impact on two response variables: electric conductivity (Y1) and final ammonium concentration (Y2). An in‐depth analysis of variance (ANOVA) was performed to examine the variables and their interactions. The results indicated that Y1 was significantly influenced by C, while Y2 was influenced by B. In addition, the predictive models demonstrated strong correlations, with a coefficient of determination (R2) greater than 0.88 for both response variables. The RSM approach applied to optimize the parameters studied identified the following optimum values: 14.17 V for A, 1 mg/L for B and 70 % for C, giving Y1 of 215.377 μS/cm and Y2 of 0.279 mg/L.
The hydroxycarboxylic acid receptor HCA2 is required for the protective effect of ketogenic diet in epilepsy
Authors: Richardson Jill C. , Higgins Guy A. , Upton Neil , Massey Peter , Cunningham Mark , Wilson Steve , Holenz Joerg , Taylor Colleen , Lavrov Arseniy , Lin Hong , Matsuoka Yasuji , Brown Andrew J. 
Published: October 2024

AbstractOne third of epilepsy patients are resistant to treatment with current anti‐seizure medications. The ketogenic diet is used to treat some forms of refractory epilepsy, but the mechanism of its action has not yet been elucidated. In this study, we aimed to investigate whether the hydroxycarboxylic acid receptor 2 (HCA2), a known immunomodulatory receptor, plays a role in mediating the protective effect of this diet. We demonstrate for the first time that selective agonists at this receptor can directly reduce seizures in animal models. Agonists also reduce network activity in rodent and human brain slices. Ketogenic diet is known to increase circulating levels of endogenous HCA2 agonists, and we show that the effect of ketogenic diet in reducing seizures in the 6 Hz seizure model is negated in HCA2‐deficient mice. Our data support the potential of HCA2 as a target for the treatment of epilepsy and potentially for neurodegenerative diseases.