bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics
Authors: Hu Qingping , Wei Jianteng , Liu Yewei , Fei Xiulan , Hao Yuwei , Pei Dong , Di Duolong 
Published: December 2016

AbstractBiomarkers involved in alcohol‐induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol‐induced oxidative stress in human liver L‐02 cells was used to discover the potential biomarkers. Metabolites from L‐02 cells induced by alcohol were measured by high‐performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least‐squares discriminate analysis). Based on the retention time, UV spectrum and LC–MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol‐induced oxidative stress, namely, malic acid, oxidized glutathione, γ‐glutamyl‐cysteinyl‐glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches.
Analytical method for urinary metabolites as biomarkers for monitoring exposure to phthalates by gas chromatography/mass spectrometry
Authors: Yoshida Toshiaki 
Published: December 2016

AbstractPhthalates, widely used as plasticizers, have been detected in indoor air, but there have been few reports on methods of analyzing urinary metabolites as biomarkers to monitor exposure to di‐n‐pentyl phthalate or di‐n‐hexyl phthalate. Presented here is a cost‐effective and sensitive analytical method for the determination of urinary metabolites of phthalates containing these two compounds. Nine urinary phthalate metabolites were enzymatically hydrolyzed and extracted with toluene: monomethyl phthalate, monoethyl phthalate, monoisobutyl phthalate, mono‐n‐butyl phthalate, mono‐n‐pentyl phthalate, mono‐n‐hexyl phthalate, monocyclohexyl phthalate, monobenzyl phthalate and mono(2‐ethyl‐5‐carboxypentyl) phthalate. After transformation to their tert‐butyldimethylsilyl derivatives, they were analyzed by gas chromatography/mass spectrometry in the electron impact ionization mode. The calibration curves for the metabolites were linear at urinary concentrations of up to 30 μg/L, showing that they could be determined accurately and precisely (detection limits 0.1–0.4 μg/L, quantification limits 0.3–1.3 μg/L). The urine samples collected could be stored for up to 1 month at −20°C. The proposed analytical method was used to examine urine samples from seven healthy volunteers. This method should be useful for monitoring phthalate exposure in the general population.
Skeletal muscle atrophy is induced by Fbxw7β via atrogene upregulation
Authors: Shin Kyungshin , Ko Young‐Gyu , Jeong Jaemin , Kwon Heechung 
Published: December 2016

AbstractMuscle atrophy decreases skeletal muscle mass and is induced by inherited cachectic symptoms, genetic disorders, and sarcopenia. However, the molecular pathways associated with the onset of muscle atrophy are still unclear. In this study, we evaluated Fbxw7β, a gene associated with the development of muscle atrophy in vitro and in vivo. Among the three Fbxw7 isoforms, ectopically overexpressed Fbxw7β induced the expression of myogenin and major atrogene markers (atrogin‐1 and MuRF‐1) and reduced myoblast differentiation. In addition, endogenous expression of Fbxw7β was also upregulated by dexamethasone, which mimics muscle atrophy in vitro, accompanied by induction of myogenin and atrogene expression in primary myoblasts. Functional analysis of Fbxw7β using short hairpin RNA (shRNA) and a dominant‐negative mutant (ΔFbox) suggested that Fbxw7β regulated muscle atrophy in vitro and in vivo. In particular, ΔFbox did not reduce the sizes of muscle fibers and did not induce myogenin and atrogene expression in vivo. Therefore, our findings demonstrated, for the first time, that Fbxw7β induced muscle atrophic phenotypes via atrogenes in adult muscle precursor cells and myofibers; this mechanism could be a potential therapeutic target for skeletal muscle atrophy.
The Chemistry of a Non‐Interacting Vicinal Frustrated Phosphane/Borane Lewis Pair
Authors: Elmer Lisa‐Maria , Kehr Gerald , Daniliuc Constantin G. , Siedow Melanie , Eckert Hellmut , Tesch Matthias , Studer Armido , Williams Kamille , Warren Timothy H. , Erker Gerhard 
Published: December 2016

AbstractThe dimesitylphosphinocyclopentene/HB(C6F5)2‐derived vicinal trans‐1,2‐P/B frustrated Lewis pair (FLP) 4 shows no direct phosphane–borane interaction. Toward some reagents it behaves similar to an intermolecular FLP; it cleaves dihydrogen, deprotonates terminal alkynes, and adds to organic carbonyl compounds including CO2. It shows typical intramolecular FLP reaction modes (cooperative 1,1‐additions) to mesityl azide, to carbon monoxide, and to NO. The latter reaction yields a persistent P/B FLPNO nitroxide radical, which undergoes H‐atom abstraction reactions. The FLP 4 serves as a template for the CO reduction by [HB(C6F5)2] to generate a FLP‐η2‐formylborane. The formylborane moiety is removed from the FLP template by reaction with pyridine to yield a genuine pyridine stabilized formylborane that undergoes characteristic borane carbaldehyde reactions (Wittig olefination, imine formation). Most new products were characterized by X‐ray diffraction.
Graphene‐Oxide‐Based Enzyme Nanoarchitectonics for Substrate Channeling
Authors: Mathesh Motilal , Liu Jingquan , Barrow Colin J. , Yang Wenrong 
Published: December 2016

AbstractThe controlled spatial organization or compartmentalization of multi‐enzyme cascade reactions to transfer a substrate from one enzyme to another for substrate channeling on scaffolds has sparked increasing interest in recent years. Here, we use graphene oxides to study the dependence of the activity of cascade reactions in a closely packed, randomly immobilized enzyme system on a 2 D scaffold. We first observe that the hydrophobicity of graphene oxides and various enzyme architectures for co‐immobilized systems are important attributes for achieving high product‐conversion rates. A transient time close to 0 s can be achieved if enzymes are randomly immobilized close to one another, owing to direct molecular channeling. This contributes to overcoming complications regarding control of the spatial arrangement of the enzymes. Furthermore, a fabricated bienzyme paper can be used for glucose detection with high stability, reusability, and enhanced substrate channeling. Our findings provide new guidance for enzyme orientation on 2 D scaffolds, which may be extrapolated to other multienzyme cascade systems.
Three‐Dimensional Fibrous Network of Na0.21MnO2 for Aqueous Sodium‐Ion Hybrid Supercapacitors
Authors: Karikalan Natarajan , Karuppiah Chelladurai , Chen Shen‐Ming , Velmurugan Murugan , Gnanaprakasam Periyasami 
Published: December 2016

AbstractSodium‐ion hybrid supercapacitors are potential energy‐storage devices and have recently received enormous interest. However, the development of cathode materials and the use of nonaqueous electrolyte remain a great challenge. Hence, aqueous Na‐ion hybrid supercapacitors based on a three‐dimensional network of NaMnO2 were developed. The cathode material was synthesized by the electro‐oxidation of potassium manganese hexacyanoferrate nanocubes. The oxidized compound was confirmed to be Na0.21MnO2 by various physical characterization methods. Manganese dioxide is a well‐characterized material for aqueous asymmetric pseudocapacitors, but its usage at high operating voltages is limited due to the electrochemical stability of water. Nevertheless, high‐potential and high‐performance aqueous supercapacitors exhibiting a cell potential of 2.7 V were developed. Further, the practical applicability of an asymmetric supercapacitor based on NaMnO2 (cathode) and reduced graphene oxide (anode) was demonstrated by powering a 2.1 V red LED.
A Simple Approach To Develop Tailored Mesoporosity in Nanostructured Heteropolysalts
Authors: Alcañiz‐Monge Juan , Trautwein Guido , El Bakkali Bouchra , Reinoso Santiago 
Published: December 2016

AbstractIn this study, we describe a very simple approach to the development of tailored mesoporosity in any nanostructured heteropolysalt with control over both the mesoporous volume and the pore size. This approach, which consists in the treatment of a solid microporous precursor with a basic agent, has been tested on the ammonium salt of the Keggin‐type [PMo12O40]3− heteropolyanion and constitutes a novel procedure for the preparation of mesoporous solids with no precedents. The results obtained in this study allow two main conclusions to be drawn: 1) the micro‐ and mesoporous structures in the heteropolysalt nanoparticles are independent from each other and 2) the development of mesoporosity in the solid material must be related to a process of alkaline degradation within the core of the nanocrystals that aggregate into the particles. These results afford valuable additional information to the present model of porosity that has been established for heteropolysalts.
The Delicate Balance of Preorganisation and Adaptability in Multiply Bonded Host–Guest Complexes
Authors: von Krbek Larissa K. S. , Achazi Andreas J. , Schoder Stefan , Gaedke Marius , Biberger Tobias , Paulus Beate , Schalley Christoph A. 
Published: December 2016

AbstractRigidity and preorganisation are believed to be required for high affinity in multiply bonded supramolecular complexes as they help reduce the entropic penalty of the binding event. This comes at the price that such rigid complexes are sensitive to small geometric mismatches. In marked contrast, nature uses more flexible building blocks. Thus, one might consider putting the rigidity/high‐affinity notion to the test. Multivalent crown/ammonium complexes are ideal for this purpose as the monovalent interaction is well understood. A series of divalent complexes with different spacer lengths and rigidities has thus been analysed to correlate chelate cooperativities and spacer properties. Too long spacers reduce chelate cooperativity compared to exactly matching ones. However, in contrast to expectation, flexible guests bind with chelate cooperativities clearly exceeding those of rigid structures. Flexible spacers adapt to small geometric host–guest mismatches. Spacer–spacer interactions help overcome the entropic penalty of conformational fixation during binding and a delicate balance of preorganisation and adaptability is at play in multivalent complexes.
Total Synthesis of Mycobacterium tuberculosis Dideoxymycobactin‐838 and Stereoisomers: Diverse CD1a‐Restricted T Cells Display a Common Hierarchy of Lipopeptide Recognition
Authors: Cheng Janice M. H. , Liu Ligong , Pellicci Daniel G. , Reddiex Scott J. J. , Cotton Rachel N. , Cheng Tan‐Yun , Young David C. , Van Rhijn Ildiko , Moody D. Branch , Rossjohn Jamie , Fairlie David P. , Godfrey Dale I. , Williams Spencer J. 
Published: December 2016

AbstractMycobacterium tuberculosis produces dideoxymycobactin‐838 (DDM‐838), a lipopeptide that potently activates T cells upon binding to the MHC‐like antigen‐presenting molecule CD1a. M. tuberculosis produces DDM‐838 in only trace amounts and a previous solid‐phase synthesis provided sub‐milligram quantities. We describe a high‐yielding solution‐phase synthesis of DDM‐838 that features a Mitsunobu substitution that avoids yield‐limiting epimerization at lysine during esterification, and amidation conditions that prevent double‐bond isomerization of the Z‐C20:1 acyl chain, and provides material with equivalent antigenicity to natural DDM‐838. Isomers of DDM‐838 that varied in stereochemistry at the central lysine and the C20:1 acyl chain were compared for their ability to be recognised by CD1a‐restricted T cell receptors (TCRs). These TCRs, derived from unrelated human donors, exhibited a similar spectrum of reactivity towards the panel of DDM‐838 isomers, highlighting the exquisite sensitivity of lipopeptide‐reactive T cells for the natural DDM stereochemistry.
Chiral Primary Amine Catalyzed Asymmetric α‐Benzylation with In Situ Generated ortho‐Quinone Methides
Authors: Zhu Yunbo , Zhang Wen‐Zhao , Zhang Long , Luo Sanzhong 
Published: December 2016

AbstractA dual activation strategy integrating primary amine catalysis and Lewis base activation has been developed for an asymmetric α‐benzylation reaction. Enamines derived from β‐ketocarbonyls could react effectively with in situ generated ortho‐quinone methides under Lewis base activation in asymmetric α‐benzylation of β‐ketocarbonyls and α‐branched aldehydes. The approach enables the creation of acyclic all‐carbon quaternary stereocenters with excellent enantioselectivities and good activity.
Frustrated Lewis Pair‐Like Reactivity of Rare‐Earth Metal Complexes: 1,4‐Addition Reactions and Polymerizations of Conjugated Polar Alkenes
Authors: Xu Pengfei , Yao Yingming , Xu Xin 
Published: December 2016

AbstractThree rare‐earth aryloxide ion pairs {[L1REOAr]+/[B(C6F5)4]−; L1=CH3C(2,6‐iPr2C6H3N)CHC(CH3)(NCH2CH2PPh2); RE=Sc, Y, Lu; Ar=2,6‐tBu2C6H3} were reported that feature rare‐earth/phosphorus (RE/P) combinations exhibiting frustrated Lewis pair (FLP)‐like 1,4‐addition reactions towards conjugated carbonyl substrates (e.g., enone, ynone, and acrylic substrates). Furthermore, these RE/P complexes were found to be effective catalysts for the polymerization of conjugated polar alkene monomers. Mechanistic studies revealed that the rare‐earth metal‐catalyzed polymerizations were initiated by new FLP‐type 1,4‐additions rather than traditional and ubiquitous covalent RE−E (E=H, C, N, etc.) bond insertion or single‐electron transfer.
DaNa 2.0 – verlässliche Informationen zur Sicherheit von marktüblichen Nanomaterialien
Authors: Steinbach Christoph , Bohmer Nils , Krug Harald F. , Kühnel Dana , Nau Katja , Paul Florian , Reithel Sarah , Marquardt Clarissa 
Published: December 2016

AbstractSowohl Wissenschaftler als auch der Bürger brauchen verlässliche Informationen, wenn sie faktenorientiert über die Sicherheit von Nanomaterialien diskutieren wollen. Nicht alle Artikel zu diesem Thema eignen sich für eine Risikoabschätzung. Deshalb ist es notwendig, geeignete von ungeeigneten Artikeln zu trennen. Dies ist das Ziel des vom Bundesministerium für Bildung und Forschung geförderten Projekts DaNa 2.0. Hier werden wissenschaftliche Veröffentlichungen in einem transparenten Verfahren unter Verwendung eines Literatur‐Kriterienkatalog evaluiert und die Ergebnisse in Form von allgemeinverständlichen Artikeln auf der Projekt‐Website publiziert.
Synuclein‐γ in uterine serous carcinoma impacts survival: An NRG Oncology/Gynecologic Oncology Group study
Authors: Winder Abigail D. , Maniar Kruti P. , Wei Jian‐Jun , Liu Dachao , Scholtens Denise M. , Lurain John R. , Schink Julian C. , Buttin Barbara M. , Filiaci Virginia L. , Lankes Heather A. , Ramirez Nilsa C. , Park Kay , Singh Meenakshi , Lieberman Richard W. , Mannel Robert S. , Powell Matthew A. , Backes Floor J. , Mathews Cara A. , Pearl Michael L. , Secord Angeles Alvarez , Peace David J. , Mutch David G. , Creasman William T. , Kim J. Julie 
Published: December 2016

BACKGROUNDSynuclein‐γ (SNCG) is highly expressed in advanced solid tumors, including uterine serous carcinoma (USC). The objective of the current study was to determine whether SNCG protein was associated with survival and clinical covariates using the largest existing collection of USCs from the Gynecologic Oncology Group (GOG‐8023).METHODSHigh‐density tissue microarrays (TMAs) of tumor tissues from 313 patients with USC were stained by immunohistochemistry for SNCG, p53, p16, FOLR1, pERK, pAKT, ER, PR, and HER2/neu. Associations of SNCG and other tumor markers with overall and progression‐free survival were assessed using log‐rank tests and Cox proportional‐hazards models, which also were adjusted for age, race, and stage.RESULTSThe overall survival at 5 years was 46% for women with high SNCG expression and 62% for those with low SNCG expression (log‐rank P = .021; hazard ratio [HR], 1.31; 95% confidence interval [CI], 0.91‐1.9 in adjusted Cox model). The progression‐free survival rate at 5 years was worse for women who had high SNCG expression, at 40%, compared with 56% for those who had low SNCG expression (log‐rank P = .0081; HR, 1.36; 95% CI, 0.96‐1.92 in adjusted Cox model). High levels of both p53 and p16 were significantly associated with worse overall survival (p53: HR, 4.20 [95% CI, 1.54‐11.45]; p16: HR, 1.95 [95% CI, 1.01‐3.75]) and progression‐free survival (p53: HR, 2.16 [95% CI, 1.09‐4.27]; p16: HR, 1.53 [95% CI, 0.87‐2.69]) compared with low levels.CONCLUSIONSThis largest collection of USCs to date demonstrates that SNCG was associated with poor survival in univariate analyses. SNCG does not predict survival outcome independent of p53 and p16 in models that jointly consider multiple markers. Cancer 2017;123:1144–1155. © 2016 American Cancer Society.
Interobserver reproducibility of cytologic p16INK4a/Ki‐67 dual immunostaining in human papillomavirus‐positive women
Authors: Benevolo Maria , Allia Elena , Gustinucci Daniela , Rollo Francesca , Bulletti Simonetta , Cesarini Elena , Passamonti Basilio , Giovagnoli Maria Rosaria , Carico Elisabetta , Carozzi Francesca M. , Mongia Alessandra , Fantacci Giulia , Confortini Massimo , Rubino Teresa , Fodero Cristina , Prandi Sonia , Marchi Natalina , Farruggio Angelo , Coccia Anna , Macrì Luigia , Ghiringhello Bruno , Ronco Guglielmo , Bragantini Emma , Polla Enzo , Maccallini Vincenzo , Negri Giovanni , Giorgi Rossi Paolo ,   for the New Technologies for Cervical Cancer Screening 2 (NTCC2) Working Group
Published: December 2016

BACKGROUNDThe accumulation of cyclin‐dependent kinase inhibitor 2A (p16ink4a) protein in a cell is associated with neoplastic progression in precancerous cervical lesions. Dual staining for p16ink4a and Ki‐67 has been proposed as a triage test in cervical cancer screening for women who test positive for human papillomavirus DNA. In this study, interobserver reproducibility of the interpretation of this test was assessed.METHODSForty‐two immunostained, liquid‐based cytology slides were divided into 2 sets and were interpreted by 17 to 21 readers from 9 different laboratories, yielding a total of 816 reports. Immunostaining results were classified as positive, negative, inconclusive, or inadequate. After evaluation of the first set of slides and before circulation of the second set, the results were discussed in a plenary meeting. The 10 slides with the most discordant results were evaluated again by selected expert cytopathologists.RESULTSThe overall κ value was 0.612 (95% confidence interval [CI], 0.523‐0.701), it was higher for the positive and negative categories (κ = 0.692 and κ = 0.641, respectively), and it was almost null for the inconclusive category (κ = 0.058). Considering only readers from laboratories with documented experience, the κ value was higher (κ = 0.747; 95% CI, 0.643‐0.839) compared with nonexperienced centers (κ = 0.498; 95% CI, 0.388‐0.616). The results were similar in both sets of slides (κ = 0.505 [95% CI, 0.358‐0.642] and κ = 0.521 [95% CI, 0.240‐0.698] for the first and second sets, respectively). Reinterpretation of the slides with the most discordant results did not provide any improvement (first evaluation, κ = 0.616 [95% CI, 0.384‐0.866]; second evaluation, κ = 0.403 [95% CI, 0.182‐0.643]).CONCLUSIONSDual staining for p16 ink4a and Ki‐67 demonstrated good reproducibility, confirming its robustness, which is a necessary prerequisite for its adoption as a triage test in cervical cancer screening programs that use human papillomavirus DNA as a primary test. Cancer Cytopathol 2017;125:212–220. © 2016 American Cancer Society.
Low latency flexible FPGA implementation of point multiplication on elliptic curves over GF(p)
Authors: Javeed Khalid , Wang Xiaojun 
Published: December 2016

SummaryElliptic curve cryptography (ECC) is a branch of Public‐Key cryptography that is widely accepted for secure data exchange in many resource‐limited devices. This paper presents a novel hardware cryptographic processor for ECC over general prime field GF(p). It is optimized on circuit level by introducing new parallel modular multiplication algorithm with its efficient hardware architecture, which offers significant improvement over the previously used techniques. Subsequently, on the system level, it is optimized by exploiting available high degree of parallelism using projective coordinates by incorporating four parallel multiplier units. The proposed hardware is implemented on Xilinx Virtex‐4 and Virtex‐6 field programmable gate arrays. A 256‐bit scalar multiplication is completed in 1.43 ms and 2.96 ms in a cycle count of 207 1K on Virtex‐6 and Virtex‐4 field programmable gate array paltforms, respectively. The Virtex‐6 implementation attains a maximum frequency of 144 MHz, occupies 32 4K look‐up‐tables, whereas on Virtex‐4 it is about 70 MHz with 35 7K slices. The results show that the proposed design offers a significant improvement in computation time with a significant reduction in cycle count as compared with the other reported designs. Therefore, it is a good choice to be used in many ECC‐based schemes. Copyright © 2016 John Wiley & Sons, Ltd.
A novel clinical score (InterTAK Diagnostic Score) to differentiate takotsubo syndrome from acute coronary syndrome: results from the International Takotsubo Registry
Authors: Ghadri Jelena R. , Cammann Victoria L. , Jurisic Stjepan , Seifert Burkhardt , Napp L. Christian , Diekmann Johanna , Bataiosu Dana Roxana , D'Ascenzo Fabrizio , Ding Katharina J. , Sarcon Annahita , Kazemian Elycia , Birri Tanja , Ruschitzka Frank , Lüscher Thomas F. , Templin Christian ,   InterTAK co‐investigators
Published: December 2016

AimsClinical presentation of takotsubo syndrome (TTS) mimics acute coronary syndrome (ACS) and does not allow differentiation. We aimed to develop a clinical score to estimate the probability of TTS and to distinguish TTS from ACS in the acute stage.Methods and resultsPatients with TTS were recruited from the International Takotsubo Registry (
www.takotsubo‐registry.com) and ACS patients from the leading hospital in Zurich. A multiple logistic regression for the presence of TTS was performed in a derivation cohort (TTS, n = 218; ACS, n = 436). The best model was selected and formed a score (InterTAK Diagnostic Score) with seven variables, and each was assigned a score value: female sex 25, emotional trigger 24, physical trigger 13, absence of ST‐segment depression (except in lead aVR) 12, psychiatric disorders 11, neurologic disorders 9, and QTc prolongation 6 points. The area under the curve (AUC) for the resulting score was 0.971 [95% confidence interval (CI) 0.96–0.98] and using a cut‐off value of 40 score points, sensitivity was 89% and specificity 91%. When patients with a score of ≥50 were diagnosed as TTS, nearly 95% of TTS patients were correctly diagnosed. When patients with a score ≤31 were diagnosed as ACS, ∼95% of ACS patients were diagnosed correctly. The score was subsequently validated in an independent validation cohort (TTS, n = 173; ACS, n = 226), resulting in a score AUC of 0.901 (95% CI 0.87–0.93).ConclusionThe InterTAK Diagnostic Score estimates the probability of the presence of TTS and is able to distinguish TTS from ACS with a high sensitivity and specificity.Trial registration: NCT0194762