bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vector‐Based Adaptive Attitude Observer and Controller on Special Orthogonal Group
Authors: Lu Xuhui , Jia Yingmin , Matsuno Fumitoshi 
Published: October 2016

AbstractAn adaptive attitude observer‐controller scheme is proposed for attitude tracking of a rigid body. In the derived observer, the vector measurements are directly utilized to estimate gyro bias, and thus estimates of gyro bias are obtained independent to attitude estimates. The proposed observer is even robust towards fluctuation of gyro bias. Then an adaptive controller is proposed on the Special Orthogonal Group to track the reference trajectory subject to uncertain inertia parameters. This controller belongs to the non‐certainty‐equivalent framework, and the vector signals are also utilized into estimation of inertia parameters. It is ensured that the estimates of inertia parameters can converge towards real values to some degrees. Simulation results compared with previous observer and controller schemes verify the effectiveness of the proposed scheme.
Heterologous expression of lipases YLIP4, YLIP5, YLIP7, YLIP13, and YLIP15 fromYarrowia lipolyticaMSR80 inEscherichia coli: Substrate specificity, kinetic comparison, and enantioselectivity
Authors: Syal Poonam , Gupta Rani 
Published: October 2016

AbstractFive lipase genes,ylip4,ylip5,ylip7,ylip13, andylip15, fromYarrowia lipolyticaMSR80 were cloned and expressed in the pEZZ18‐HB101 system. The lipases shared maximum sequence identity withCandida gallilipase, whereas they shared structural similarity with YLIP2 ofY. lipolyticaCLIB122. The enzymes, purified using IgG sepharose, had specific activities in the range of 7–25 U mg−1. Biochemical characteristics of all the lipases varied with respect to thermostability, substrate specificity, and enantioselectivity. All the enzymes were most active at neutral or slightly alkaline pH and were stable in the pH range 3.0–8.0, except YLIP4, which showed 50% stability at pH 10.0. Temperature optima of all the lipases varied from 30 to 50 ºC. YLIP15 and YLIP13 were most thermostable with at1/2of 138 and 112 Min, respectively, at 60 °C. The lipases exhibited varied substrate specificity onp‐nitrophenyl esters ranging from short‐chain specificity (YLIP15), mid‐chain specificity (YLIP4, YLIP5, YLIP7), and long‐chain specificity (YLIP13). Catalytic efficiency onp‐nitrophenylcaprate was highest for YLIP13 (67 × 103mM−1min−1) and lowest for YLIP15 (6.7 × 103mM−1min−1). YLIP13 was S‐enantioselective, and YLIP15 was R‐enantioselective with enantiomeric excess of 53 and 36%, respectively. Of all five lipases, YLIP13 and YLIP15 could be considered as industrially important enzymes as they were thermostable and enantioselective.
Mangiferin: A xanthonoid with multipotent anti‐inflammatory potential
Authors: Saha Sukanya , Sadhukhan Pritam , Sil Parames C. 
Published: October 2016

AbstractOver the last era, small molecules sourced from different plants have gained attention for their varied and long‐term medicinal benefits. Their advantageous therapeutic effects in diverse pathological complications lead researchers to give an ever‐increasing emphasis on them and discover their novel therapeutic potentials. Among these, the heat stable, xanthonoid group of organic molecules has gained special importance with distinctive regards to the bioactive molecule mangiferin due to its solubility in water. Mangiferin, a yellow polyphenol having C‐glycosyl xanthone structure, is widely present in different edible sources like mango, and possesses numerous biological activities. Extensive research with this molecule shows its antioxidant, anti‐inflammatory, antidiabetic, anticancer, antimicrobial, analgesic, and immunomodulatory properties. Thus, it provides protection against a wide range of physiological disorders. The C‐glucosyl linkage and polyhydroxy groups in mangiferin's structure contribute essentially to its free radical‐scavenging activity. Moreover, its ability in regulating various transcription factors like NF‐κB, Nrf‐2, etc. and modulating the expression of different proinflammatory signaling intermediates like tumor necrosis factor‐α, COX‐2, etc. contribute to its anti‐inflammatory, anticancer, and antidiabetic potentials. In this comprehensive article, information has been provided about the sources, chemical structure, metabolism, and different biological activities of mangiferin with special emphasis on the underlying cellular signal transduction pathways. Insights into an in‐depth assessment of mangiferin's anti‐inflammatory therapeutic potential have also been discussed in detail. On an overall perspective, this review aims to stage mangiferin's diversified therapeutic applications and its emerging possibility as a promising drug in future based on its anti‐inflammatory property. © 2016 BioFactors, 42(5):459–474, 2016
The potential role of mangiferin in cancer treatment through its immunomodulatory, anti‐angiogenic, apoptopic, and gene regulatory effects
Authors: Núñez Selles Alberto J. , Daglia Maria , Rastrelli Luca 
Published: October 2016

AbstractMangiferin (1,3,6,7‐tetrahydroxyxanthone‐C2‐β‐D‐glucoside) is a natural bioactive xanthonoid that can be found in many plant species, among which the mango tree (Mangifera indica L), a plant widely used in the traditional medicinal, is one of its primary sources. The use of mangiferin for cancer treatment has attracted the attention of research groups around the World. Single administration of mangiferin or in combination with known anticancer chemicals has shown the potential benefits of this molecule in lung, brain, breast, cervix, and prostate cancers, and leukemia. Mangiferin mechanisms of action against cancer cells through in vitro, ex vivo, or in vivo models are discussed besides its antioxidant and anti‐inflammatory properties. Nevertheless, pharmaceutical development and, therefore, clinical trials on cancer targets are still lacking. © 2016 BioFactors, 42(5):475–491, 2016
Integrated process design for biocatalytic synthesis by a Leloir Glycosyltransferase: UDP‐glucose production with sucrose synthase
Authors: Schmölzer Katharina , Lemmerer Martin , Gutmann Alexander , Nidetzky Bernd 
Published: October 2016

ABSTRACTNucleotide sugar‐dependent (“Leloir”) glycosyltransferases (GTs), represent a new paradigm for the application of biocatalytic glycosylations to the production of fine chemicals. However, it remains to be shown that GT processes meet the high efficiency targets of industrial biotransformations. We demonstrate in this study of uridine‐5′‐diphosphate glucose (UDP‐glc) production by sucrose synthase (from Acidithiobacillus caldus) that a holistic process design, involving coordinated development of biocatalyst production, biotransformation, and downstream processing (DSP) was vital for target achievement at ∼100 g scale synthesis. Constitutive expression in Escherichia coli shifted the recombinant protein production mainly to the stationary phase and enhanced the specific enzyme activity to a level (∼480 U/gcell dry weight) suitable for whole‐cell biotransformation. The UDP‐glc production had excellent performance metrics of ∼100 gproduct/L, 86% yield (based on UDP), and a total turnover number of 103 gUDP‐glc/gcell dry weight at a space‐time yield of 10 g/L/h. Using efficient chromatography‐free DSP, the UDP‐glc was isolated in a single batch with ≥90% purity and in 73% isolated yield. Overall, the process would allow production of ∼0.7 kg of isolated product/L E. coli bioreactor culture, thus demonstrating how integrated process design promotes the practical use of a GT conversion. Biotechnol. Bioeng. 2017;114: 924–928. © 2016 Wiley Periodicals, Inc.
Engineering three dimensional micro nerve tissue using postnatal stem cells from human dental apical papilla
Authors: Kim Byung‐Chul , Jun Sung‐Min , Kim So Yeon , Kwon Yong‐Dae , Choe Sung Chul , Kim Eun‐Chul , Lee Jae‐Hyung , Kim Jinseok , Suh Jun‐Kyo Francis , Hwang Yu‐Shik 
Published: October 2016

ABSTRACTThe in vitro generation of cell‐based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D nerve tissue formation of pluripotent stem cells to study underlying biology in nerve development, cell‐based nerve tissues have not been developed using human postnatal stem cells with therapeutic potential. Here, we established a culture strategy for the generation of in vitro cell‐based 3D nerve tissue from postnatal stem cells from apical papilla (SCAPs) of teeth, which originate from neural crest‐derived ectomesenchyme cells. A stem cell population capable of differentiating into neural cell lineages was generated during the ex vivo expansion of SCAPs in the presence of EGF and bFGF, and SCAPs differentiated into neural cells, showing neural cell lineage‐related molecular and gene expression profiles, morphological changes and electrophysical property under neural‐inductive culture conditions. Moreover, we showed the first evidence that 3D cell‐based nerve‐like tissue with axons and myelin structures could be generated from SCAPs via 3D organotypic culture using an integrated bioprocess composed of polyethylene glycol (PEG) microwell‐mediated cell spheroid formation and subsequent dynamic culture in a high aspect ratio vessel (HARV) bioreactor. In conclusion, the culture strategy in our study provides a novel approach to develop in vitro engineered nerve tissue using SCAPs and a foundation to study biological events in the neural differentiation of postnatal stem cells. Biotechnol. Bioeng. 2017;114: 903–914. © 2016 Wiley Periodicals, Inc.
MiR‐17‐5p promotes cancer cell proliferation and tumorigenesis in nasopharyngeal carcinoma by targeting p21
Authors: Chen Chun , Lu Zenghong , Yang Jie , Hao Weichao , Qin Yujuan , Wang Huiyan , Xie Congying , Xie Raoying 
Published: October 2016

AbstractMicroRNAs (miRNAs) may act as either tumor suppressors or oncogenes in various types of cancers. Previous studies have indicated that miR‐17‐5p is involved in the initiation and development of human tumors. However, its mechanism and function in nasopharyngeal carcinoma (NPC) remain largely unclear. In this study, we evaluated the expression profiles of miR‐17‐5p and p21 in NPC cell lines and tissues by quantitative real‐time PCR (qRT‐PCR). For the analysis, we have established a stable overexpression or depletion of miR‐17‐5p NPC cell lines for analyzing the effects of cell proliferation by MTT, colony formation, and cell cycle assay. A nude mice xenograft model was used to verify the tumor growth in vivo. MiR‐17‐5p was overexpressed, whereas the expression of p21 was downregulated in NPC cell lines and tissues. The miR‐17‐5p expression level was inversely correlated with the p21 mRNA level in NPC samples. Furthermore, analysis of 2−ΔΔCt value in 81 NPC patients suggested that the elevated expression level of miR‐17‐5p or the downregulated expression level of p21 was significantly correlated with tumor size (T classification) and tumor stage, and Kaplan–Meier survival analysis revealed a correlation between miR‐17‐5p or p21 expression level and overall survival times in 81 NPC patients. MiR‐17‐5p promoted cell growth in vivo and in vitro by directly targeting p21. Our results indicate that miR‐17‐5p can promote the occurrence of NPC and it may serve as a potential novel diagnostic maker or therapeutic target for NPC in the future.
Positioning of second‐line treatment for advanced gastric and gastroesophageal junction adenocarcinoma
Authors: Pericay Carles , Rivera Fernando , Gomez‐Martin Carlos , Nuñez Inmaculada , Cassinello Alejo , Imedio Esteban Rodrigo 
Published: October 2016

AbstractTumors of the upper gastrointestinal tract are increasing in incidence; yet, approaches to the treatment of advanced gastric and/or gastroesophageal junction cancer vary widely, with no internationally agreed first‐line regimens. Recent clinical trials have shown that second‐line treatment is now possible for selected patients with advanced disease, and current data suggest that the combination of ramucirumab plus paclitaxel may become a standard of care in the second‐line setting for metastatic gastric cancer. Several prognostic factors have been identified for overall survival in the second‐line setting; this emphasizes the need for careful sequencing of all treatments to ensure that individual patients receive optimum care. This article reviews published data on the treatment of advanced gastric cancer, with a particular emphasis on second‐line chemotherapy, and suggests treatment sequences based on current understanding.
Gender mobility in the countertransference: An autoethnographic investigation
Authors: Ivey Gavin 
Published: October 2016

AbstractAimsThe primary aim of this study was to investigate a psychotherapist's countertransference experience of gender mobility in response to a patient using projective identification to maintain a rigidly defended sexual identity. A secondary aim was to explore the utility of autoethnography as a psychotherapy research method, with a specific focus on understanding the complexities of countertransference experience.MethodThe therapist's experience of gender mobility in response to the deployment and withdrawal of a female patient's projective identification is systematically tracked using thick description of session vignettes and autoethnographic self‐analysis.FindingsAutoethnography provides a viable form of practice‐led qualitative research, particularly useful in exploring countertransference experience. The contribution of the therapist's historical subjectivity and cultural positioning undercuts the asocial depiction of projective identification as largely the patient's creation. The therapist's own gender‐related conflicts are activated and contribute to the patient's projective evacuation of gendered self‐representations. The gendered complexity of the psychotherapeutic interaction defines not only defended identity, but also the therapeutic reclamation of projected self aspects in the course of treatment. Both patient and therapist experience an enlarged repertoire of sex and gender possibilities as a result of successful psychotherapy.Implications for practiceGender mobility in the countertransference experience requires considerable self‐analysis in order to determine the therapist's unconscious contribution to the projective identification process. Awareness of the social context informing the therapist's gender construction can assist in facilitating countertransference understanding. Therapists’ openness to their gendered multiplicity is critical in aiding patients who manifest some form of gender or sexual orientation conflict.
A prospective evaluation of a standardized strategy for the use of a polymeric everolimus‐eluting bioresorbable scaffold in ST‐segment elevation myocardial infarction: Rationale and design of the BVS STEMI STRATEGY‐IT study
Authors: Ielasi Alfonso , Varricchio Attilio , Campo Gianluca , Leoncini Massimo , Cortese Bernardo , Vicinelli Paolo , Brugaletta Salvatore , di Uccio Fortunato Scotto , Latib Azeem , Tespili Maurizio 
Published: October 2016

ObjectivesTo assess the feasibility and the clinical results following a prespecified bioresorbable vascular scaffold (Absorb BVS) implantation strategy in ST‐elevation myocardial infarction (STEMI) patients.BackgroundsConcerns raised about the BVS safety in STEMI setting because a not negligible thrombosis rate was reported within 30 days and 12 months after implantation. Technical procedural issues related to the structural BVS features were advocated as probable causes for the thrombotic events.MethodsThis is an investigators‐owned and ‐directed, prospective, nonrandomized, single‐arm multicenter registry intended to obtain data from 500 consecutive STEMI patients undergoing primary PCI with BVS (1.1 or GT1) following a prespecified implantation protocol. The study is recorded in ClinicalTrials.gov with the identifier: NCT02601781.ResultsThe primary endpoint is a device‐oriented composite end‐point (DOCE) of cardiac death, any myocardial infarction clearly attributable to the intervention culprit vessel and ischemic‐driven target lesion revascularization within 30 days after the index procedure. The DOCE will be assessed even at 6‐month, 1‐, 3‐, and 5‐year follow‐up.ConclusionsThis will be the first study investigating the feasibility and the early‐ and long‐term clinical impact of a prespecified BVS implantation protocol in thrombotic lesions causing STEMI. Here, we describe the rationale and the design of the study. © 2016 Wiley Periodicals, Inc.
A case of veno‐venous extracorporeal membrane oxygenation for severe respiratory failure in a superobese patient
Authors: Belliato Mirko , Cremascoli Luca , Aliberti Anna , Pagani Michele , Pellegrini Carlo , Iotti Giorgio Antonio 
Published: October 2016

Key Clinical MessageAfter risk assessment, veno‐venous extracorporeal membrane oxygenation (ECMO) has been achieved in a superobese adult patient as a bridge to recovery of respiratory failure, despite the weight‐related difficulties. Early v‐v ECMO implantation could be considered to support and to conduct weaning both from sedation and from invasive mechanical ventilation, with the goal to perform physiokinesitherapy during awake ECMO.
3 D Ordered Macroporous Alumina–Carbon Nanocomposite Supported Platinum Nanoparticles as Effective and Reusable Catalysts for Asymmetric Hydrogenation
Authors: Yang Ting , Lou Lan‐Lan , Yu Wenjun , Feng Yajun , Li Hao , Yu Kai , Liu Shuangxi 
Published: October 2016

AbstractThe three‐dimensionally ordered macroporous alumina‐carbon composite materials were firstly prepared through a surfactant‐assisted colloidal crystal template method and applied to support Pt nanoparticles. The results of SEM, XRD, TEM, and N2 sorption analyses revealed that these composite materials possessed highly ordered macroporous structures with meso‐ and micropores within the skeletons of macropores and that the Pt nanoparticles were well dispersed on these materials with a very narrow size distribution. These prepared Pt nanocatalysts were evaluated in the asymmetric hydrogenation of ethyl pyruvate after chiral modification with cinchonidine. Excellent catalytic activity and enantioselectivity were achieved. More importantly, these catalysts exhibited extraordinary stability and reusability during the reaction and could be reused at least 26 times without significant loss in catalytic activity and enantioselectivity.
Bioelectrochemical Systems as a Multipurpose Biosensing Tool: Present Perspective and Future Outlook
Authors: Grattieri Matteo , Hasan Kamrul , Minteer Shelley D. 
Published: October 2016

AbstractMicrobial bioelectrocatalysis, the process of utilizing an intact microorganism for catalyzing redox reactions, has been rapidly expanding over the last 15 years. Although microbial bioelectrocatalysis has been primarily studied for power generation and wastewater treatment, this Minireview will focus on the use of bioelectrochemical systems (BESs) for biosensing applications. This will include sensors for water quality, corrosion, and toxic shock. We will also discuss the transition of BESs to photo‐BESs and discuss their recent applications in sensing. Finally, we will discuss the future outlook for microbial bioelectrocatalysis for biosensing applications.
Aqueous Dispersions of Graphene from Electrochemically Exfoliated Graphite
Authors: Sevilla Marta , Ferrero Guillermo A. , Fuertes Antonio B. 
Published: October 2016

AbstractA facile and environmentally friendly synthetic strategy for the production of stable and easily processable dispersions of graphene in water is presented. This strategy represents an alternative to classical chemical exfoliation methods (for example the Hummers method) that are more complex, harmful, and dangerous. The process is based on the electrochemical exfoliation of graphite and includes three simple steps: 1) the anodic exfoliation of graphite in (NH4)2SO4, 2) sonication to separate the oxidized graphene sheets, and 3) reduction of oxidized graphene to graphene. The procedure makes it possible to convert around 30 wt % of the initial graphite into graphene with short processing times and high yields. The graphene sheets are well dispersed in water, have a carbon/oxygen atomic ratio of 11.7, a lateral size of about 0.5–1 μm, and contain only a few graphene layers, most of which are bilayer sheets. The processability of this type of aqueous dispersion has been demonstrated in the fabrication of macroscopic graphene structures, such as graphene aerogels and graphene films, which have been successfully employed as absorbents or as electrodes in supercapacitors, respectively.
Real‐Time NMR Studies of Oxyamine Ligations of Reducing Carbohydrates under Equilibrium Conditions
Authors: Baudendistel Oliver R. , Wieland Daniel E. , Schmidt Magnus S. , Wittmann Valentin 
Published: October 2016

AbstractLigation reactions at the anomeric center of carbohydrates have gained increasing importance in the field of glycobiology. Oxyamines are frequently used in labeling, immobilization, and bioconjugation of reducing carbohydrates. Herein, we present a systematic investigation of these ligation reactions under aqueous conditions. A series of four unprotected monosaccharides (glucose, N‐acetylglucosamine, mannose, and 2‐deoxyglucose) and one disaccharide (N,N’‐diacetylchitobiose) was reacted with three primary and one secondary oxyamine. We monitored the concentrations of the starting materials and products by 1H NMR spectroscopy and determined reaction times and equilibrium yields. Our experiments show that the outcome of the ligation reaction is not only dependent on the sugar and oxyamine used but also strongly on the reaction conditions. In the case of glucose, lowering the pH from 6 to 3 led to steadily increasing reaction rates, whereas the yields were decreasing at the same time. Variation of the temperature did not only influence the product ratio in equilibrium but can also have a strong impact on the equilibrium yield. In the case of reactions of a primary oxyamine, increased temperatures led to a higher proportion of acyclic products. Reaction of the secondary oxyamine with glucose unexpectedly led to lower yields at higher temperatures.
3‐tert‐Butyl‐Substituted Cyclohexa‐1,4‐dienes as Isobutane Equivalents in the B(C6F5)3‐Catalyzed Transfer Hydro‐tert‐Butylation of Alkenes
Authors: Keess Sebastian , Oestreich Martin 
Published: October 2016

AbstractCyclohexa‐1,4‐dienes with a tert‐butyl group at C3 are shown to function as isobutane equivalents when activated by the strong boron Lewis acid tris(pentafluorophenyl)borane. The hitherto unprecedented transfer hydro‐tert‐butylation from one unsaturated hydrocarbon to another is achieved with 1,1‐diarylalkenes as substrates, thereby presenting itself as a new way of incorporating tertiary alkyl groups into carbon frameworks. Transient carbocation intermediates give rise to competing reaction pathways that could not be fully suppressed.
Perfluoroalkylation of Alkenes by Frustrated Lewis Pairs
Authors: Behrends Ilona , Bähr Susanne , Czekelius Constantin 
Published: October 2016

AbstractThe activation of perfluoroalkyl iodides by the frustrated Lewis pair tris(pentafluorophenyl)borane and tri‐tert‐butylphosphine is described. By abstraction of both a fluorine and an iodine atom, an iodophosphonium fluoroborate salt is formed. In the presence of alkenes the corresponding iodoperfluoroalkylation products are generated regioselectively. First mechanistic investigations support a radical mechanism.