bluuebunny/crossref_metadata_2025_split huggingface dataset:
The isolation and identification of 4,8,12-trimethyltridecanoic acid from butterfat
Authors: Hansen R. P. 
Published: May 2017

SummaryThe isoprenoid fatty acid 4,8,12-trimethyltridecanoic acid has been isolated from butterfat and identified. This acid was found to be a DD diastereoisomer, and was thought to have been derived from the phytol moiety of chlorophyll. It was estimated that in the sample of butterfat investigated, 4,8,12-trimethyltridecanoic acid constituted about 0·005% of the total weight of fatty acids.
Lactose, lactic acid and mineral equilibria in Cheddar cheese manufacture
Authors: Czulak J. , Conochie J. , Sutherland B. J. , Van Leeuwen H. J. M. 
Published: May 2017

SummaryIn experimental small-scale manufacture of Cheddar cheese a study was made of the movement of lactose, lactic acid, calcium and phosphorus between curd and whey, and of the effect of high acidity developed in the curd before whey separation, on the pH and physical properties of the cheese. It was found that while the curd particles remain in contact with the whey, the lactose fermented in the curd is replaced by lactose diffusing from the whey. Lactic acid produced in the curd diffuses into the whey rather slowly.When a high lactic acid level is attained rapidly the curd does not cheddar well, the pH of the cheese is low, the flavour is sour and bitter, the body is crumbly and the cheese is bleached. When a high lactic acid level is reached slowly, the curd also does not cheddar properly but the pH of the cheese may be within normal limits up to 3 weeks, at which age its body is coherent and its colour is normal. However, on maturing, the cheese becomes acid, the body crumbly and liquid separation occurs even at 2 months of age. This points to a risk in grading cheese at an early age.It is suggested that the abnormal behaviour in cheddaring of ‘high-acid’ curds is connected with an excessive loss of calcium. When high acidity is developed over an extended time in the whey, the curd retains more lactose but loses more phosphorus so that its buffering capacity is reduced. Cheese made from such curd becomes highly acid at maturity and this leads to the development of a bitter flavour.It is, therefore, concluded that both the rate of development of acidity, and the time the curd is in the whey, are critical factors in controlling the quality of Cheddar cheese.
Carotenoid and tocopherol levels in the serum of apparently healthy dairy cattle
Authors: Bayfield R. F. , Mylrea P. J. 
Published: May 2017

Total carotenoids, β-carotene and α-tocopherol concentrations were determined in serum from healthy dairy cattle of various ages, grazing good quality pasture, in different areas of New South Wales. The relationships of these components with each other and with age of the animal were examined.Mean serum β-carotene concentrations for heifers and cows were 0·68 and 1·20mg/100ml, respectively. These concentrations were somewhat higher than those found for calves (0·20mg/100ml) and weaners (0·37 mg/100 ml). Total corresponding carotenoid values were generally about 20% higher than those for β-carotene for herds.Mean serum α-tocopherol concentrations for heifers and cows were 0·54 and 0·90 mg/100ml, respectively. Again, these values were higher than those found for calves (0·22 mg/100 ml) and weaners (0·37 mg/100 ml).There was a close relationship between serum α-tocopherol and β-carotene (r=0·90), between serum α-tocopherol and total carotenoids (r=0·92), and between serum β-carotene and total carotenoids (r= 0·99).
Buellia Violaceofusca, A New Lichen From Sweden
Authors: Thor G. , Muhr L.E. 
Published: May 2017

AbstractA sterile, sorediate, corticolous, crustose lichen from Central Sweden is tentatively assigned to the genus Buellia as B. violaceofusca sp. nov. It grows on the bark of old trunks of Quercus robur in moist, dense woods dominated by that tree.
Impedance Measurement of Thallus Moisture Content in Lichens
Authors: Coxson Darwyn S. 
Published: May 2017

AbstractThe indirect determination of water content in lichen thalli by means of impedance measurement is described. This technique applies an alternating current between small clips placed at the margins of lichen thalli. Two zones of sensitivity were apparent in measurements with the foliose lichen Xanthoparmelia chlorochroa. Ready discrimination was provided between fully saturated thalli and thalli with standing surface water films. Similarly, this technique proved highly accurate as a measurement of thallus moisture content at water potentials of –2·5 MPa and less (between 0 and 40% water content by weight in X. chlorochroa). Resolution was lower at intermediate water contents (c. 60–100Δ water content by weight), although general trends were still clearly evident. This technique is ideally suited for unattended measurements of water potential in lichens using currently available data–logger technology and provides high resolution within the range of water potentials crucial to the control of physiological activity in lichens
Physiology of the aleurone layer and starchy endosperm during grain development and early seedling growth: new insights from cell and molecular biology
Authors: Ritchie Sian , Swanson Sarah J. , Gilroy Simon 
Published: May 2017

AbstractCereal grain germination and early seedling growth involve the co-ordinated action of endosperm and embryo tissues to mobilize the storage reserves of the starchy endosperm. This mobilization is accomplished by hydrolases secreted from the aleurone and scutellar tissues. The breakdown products are then transported to the growing seedling by the scutellum. This resource-harvesting system is regulated at multiple levels. One well-defined aspect of control is brought about by the hormone gibberellin (GA). Gibberellin is released from the embryo upon imbibition and activates the aleurone cells. The secretory apparatus of the aleurone then proliferates, supporting increased hydrolase synthesis and secretion to degrade the starchy endosperm. The molecules that regulate this response to GA are now being increasingly characterized. Elements such as cGMP, calcium, calmodulin and protein kinases are well known as regulators in other eukaryotic cell types and are emerging as key control factors in the aleurone hormone response. However, superimposed upon this molecular regulatory system is another level of control, the structural pattern of tissues and stored macro- molecules that was laid down during grain development. It is the interaction of these structural motifs combined with the molecular regulatory mechanisms that ensure the appropriate timing and positioning of hydrolase production and endosperm reserve mobilization. This integrated control system ensures an extended release of nutrients to fuel early seedling growth.
Putative desiccation tolerance mechanisms in orthodox and recalcitrant seeds of the genusAcer
Authors: Greggains Valerie , Finch-Savage William E. , Quick W. Paul , Atherton Neil M. 
Published: May 2017

AbstractRecalcitrant seeds are shed moist from the plant and do not survive desiccation to the low moisture contents required for prolonged storage. It has been widely hypothesised that during desiccation of these seeds a stress induced metabolic imbalance develops that leads to free radical mediated damage and viability loss. We investigated this hypothesis in a comparison of two sympatric species ofAcerduring late seed development and post-harvest desiccation:A. platanoides(Norway maple) has orthodox seeds andA. pseudoplatanus(sycamore) has recalcitrant seeds. In both species, respiration rates declined to similar levels at shedding, and the extent of defences against free radicals appears no less in sycamore than that in Norway maple. During drying there was no evidence for the accumulation of a stable free radical, increased lipid peroxidation or decline in free radical scavenging enzymes in either species. In addition, there was a very similar, large increase in total tocopherol in both species. This increase in sycamore was largely of alpha-tocopherol, whereas in Norway maple the increase was largely from its precursor, gamma-tocopherol. Arguably this suggests a similar mechanism in both species, but increased oxidative stress in sycamore. In general, the results suggest that, although damage resulting in viability loss was clearly taking place, the limitation to desiccation tolerance did not result from inadequate free radical scavenging. Soluble carbohydrates and dehydrin-like proteins were also measured during late seed development and drying in sycamore and Norway maple. The greater concentrations of sucrose, raffinose and stachyose and amounts of dehydrins in the radicles and cotyledons of Norway maple compared with those in sycamore was consistent with greater desiccation tolerance in the former. Sycamore seeds are dormant and at the tolerant end of the continuum of desiccation sensitivity among recalcitrant species, and this may account for their different response to that of the seeds of other more sensitive recalcitrant species studied.
The effects of mycofloral infection on the viability and ultrastructure of wet-stored recalcitrant seeds of Avicennia marina (Forssk.) Vierh.
Authors: Calistru Claudia , McLean Michelle , Pammenter N.W. , Berjak Patricia 
Published: May 2017

AbstractThree questions are considered in the context of the possible effects of seed-associated mycoflora, typified by Fusarium moniliforme, during hydrated storage of recalcitrant propagules of the tropical species, Avicennia marina. These pertain to storage lifespan, whether seed susceptibility to fungal attack changes and the possibility of discriminating ultrastructurally between inherent deteriorative changes and those that are fungallyinduced. The data indicate unequivocally that if fungal activity is curtailed, then the hydrated storage lifespan of A. marina seeds can be considerably extended. When inoculated immediately with F. moniliforme, newly harvested seeds were extremely susceptible to the adverse effects of the fungus, while seeds that had been wet-stored for 4 d showed a considerably heightened resilience to the effects of the fungus when inoculated at that stage. The enhanced resilience, although declining, persisted in seeds stored hydrated for up to 10 d prior to inoculation, being lost after 12 d. After 14 d of hydrated storage, seeds became more susceptible to the effects of the fungus than those in the newly harvested condition. The resilience of seeds that had been stored in the short-term was associated with ultrastructural changes indicative of enhanced metabolic activity associated with the onset of germination. However, with the sustained stress imposed by wet-storage conditions, the seeds became increasingly badly affected by the fungus. While it was not possible to discriminate with certainty among deteriorative subcellular events ascribable to inherent deterioration or the effects of the fungus, it is concluded that a comparison of the timing of the onset of degeneration may well be diagnostic of its cause.
‘Sub-imbibed’ storage is not an option for extending longevity of recalcitrant seeds of the tropical species, Trichilia dregeana Sond.
Authors: Drew Penelope J. , Pammenter N.W. , Berjak Patricia 
Published: May 2017

AbstractRecalcitrant seeds of Trichilia dregeana were stored at 16 or 25°C, either at the water content at which they were shed or partially dried. Although having been exposed to a short period (approx. 6 h) at temperatures up to 30°C prior to storage, seeds at the original water content maintained viability for several weeks at 16°C. However, storage of undried seeds at 25°C was deleterious within 8 d, indicating a chemical basis for degeneration of hydrated recalcitrant seeds. Seeds that had been mildly dehydrated to the relatively high axis level of 1.68 g H2O g—1 dry mass, while maintaining full germinability immediately after drying, exhibited only 4% viability after 8 d in storage at 16°C and had completely lost viability after the same storage period at 25°C. Ultrastructural features characterizing hydrated seeds included indications of enhanced activity associated with initial exposure to the elevated temperature as well as some signs of stress. However, over an effective 15 d storage period at 16°C, ultrastructural features showed the cells to have retained little damage and to have been in an enhanced state of activity commensurate with ongoing development towards germination. After a longer storage period, however, signs of damage, including indirect evidence for disarray of the cytoskeleton in some axis cells, became apparent in line with the declining seed viability. Immediately following dehydration from an average axis water content of 1.97 to 1.68 H2O g g—1(the sub-imbibed condition), some ultrastructural abnormalities were apparent, but the seeds remained 100% germinable. However, within the 8 d storage period in this sub-imbibed condition, a spectrum of severe ultrastructural degeneration, including indirect evidence of the collapse of the nucleoskeleton and extensive cell lysis, accompanied viability decline of the seeds to 4%.
Desiccation tolerance in relation to soluble sugar contents in seeds of ten coffee (CoffeaL.) species
Authors: Chabrillange Nathalie , Dussert Stéphane , Engelmann Florent , Doulbeau Sylvie , Hamon Serge 
Published: May 2017

AbstractLarge differences in seed desiccation sensitivity have been observed previously among ten coffee species (Coffea arabica, C. brevipes, C. canephora, C. eugenioides, C. humilis, C. liberica, C. pocsii, C. pseudo-zanguebariae, C. sessiliflora and C.stenophylla). Of these species,C. libericaandC. humiliswere the most sensitive to desiccation andC. pseudozanguebariaethe most tolerant. A study was carried out using the same seed lots to investigate if these differences in desiccation tolerance could be correlated with differences in soluble sugar content. Soluble sugars were extracted from dry seeds and analysed using high performance liquid chromatography. The seed monosaccharide (glucose and fructose) content was very low (1.5 to 2 mg g-1dry weight [dw]) in all species studied. The sucrose content ranged from 33 mg g-1dw inC. libericaseeds to 89 mg g-1dw in seeds ofC. pocsii. Raffinose was detected in the seeds of only five species (C.arabica, C.brevipes, C.humilis, C.sessiliflora, C.stenophylla), among which only three species (C.arabica, C.sessilifloraandC.brevipes) also contained stachyose. Both raffinose and stachyose were present in very low quantities (0.3–1.4 mg g-1dw and 0.1–0.7 mg g-1dw, respectively). Verbascose was never detected. No significant relationship was found between seed desiccation sensitivity and: (i) the sugar content; (ii) the presence/absence of oligosaccharides; and (iii) the oligosaccharide:sucrose ratio.
Physiological responses to divergent selection for daily food intake or lean growth rate in pigs
Authors: Cameron N.D. , McCullough E. , Troup K. , Penman J.C. 
Published: May 2017

AbstractCorrelated responses in physiological traits to divergent selection on components of efficient lean growth identified physiological traits for use as predictors of genetic merit and provided a biological explanation for the between-selection line differences in protein and lipid deposition. Responses (differences between high (H) and low (L) selection lines) in protein metabolism during ad-libitum feeding were associated with divergent selection for daily food intake (DFI) (reduced serum creatinine concentration (H = 1·30 v. L = 1·56, s.e.d. 0·08 mg/dl)) and for lean growth rate on an ad-libitum feeding regime (LGA) (increased serum urea (H = 48 v. L = 36, s.e.d. 4 mg/dl) and creatinine (H = 1·74 v. L = 1·45 mg/dl)) concentrations, but not with selection for lean growth on a restricted feeding regime (LGS). Following 24-h fasting, responses in lipid metabolism, in the form of higher serum non-esterified fatty acid concentrations, were detected with divergent selection for both LGA (399 v. 248, s.e.d. 66 µmol/l) and LGS (H = 361 v. L = 107 µmol/l). The high LGS line appeared to ‘preserve’ protein to a greater extent than the high LGA line and similarly there was greater maintenance of lipid depots by the low LGS line compared with the low LGA line. A tentative ranking of the two pairs of lean growth selection lines on the basis of ‘importance’ of protein deposition would be high LGS, high LGA, low LGA and low LGS.Coheritabilities for serum creatinine concentration with predicted lysine balance and lysine required for protein deposition (-0·17 and 0·17, s.e. 0·08) indicated that serum creatinine concentration may usefully be included in breeding value prediction for lysine requirement and protein deposition to increase the accuracy of predicted genetic merit.Fasting did not increase the coheritabilities for serum creatinine concentration, so inclusion of serum creatinine concentration in a selection criterion for dietary lysine requirement or protein deposition does not require withdrawal of food before blood sampling animals.
Diet selection by groups of pigs: effect of a trained individual on the rate of learning about novel foods differing in protein content
Authors: Morgan C.A. , Kyriazakis I. , Lawrence A.B. , Chirnside J. , Fullam H. 
Published: May 2017

AbstractChoice feeding could be more widely applied with pigs if the initial variability between individuals in diet selection was overcome and there was a better understanding of the factors affecting the learning process in groups. In six replicates, two groups of four pigs (live weight 35·5±0·95 kg) were formed containing either an individual trained to select between two foods or an untrained control animal. The groups were offered food L (130 g crude protein (CP) and 5·6 g lysine per kg) and food H (260 g CP and 15·5 g lysine per kg) in two troughs as a choice for a period of 14 days. Food intake was measured twice daily for the first 3 days and then daily. The pigs were weighed three times per week and feeding behaviour was video recorded on the 1st and 3rd days of grouping. There was no effect of the trained pig on the performance of the group over the 2-week period (daily live-weight gain: 1·19 kg and 1·21 kg for the pigs in control or trained groups; s.e.d. 0·057). Within the first 8-h period of food recording pigs in groups with a trained pig selected a diet similar to that of the trained pig, whereas those with a control pig showed initial variation in selection before adopting a pattern in favour of one food, usually after about 3 days. In the first 24 h the pattern of visits to the troughs by the naïve pigs followed that of the trained pig whereas the pigs in the control groups initially visited the troughs at random. After 3 days the difference between the groups was small and largely non-significant. In five of the six replicates the groups selected a diet comprising 0·65H: 0·35L, which was similar to that expected from their lysine requirements. The two groups in the other replicate selected more of food L and this and the other findings are discussed in relation to the influence of social learning, previous nutritional experience and food composition on diet selection by pigs.
Effects of metabolizable protein on intake and milk production of dairy cows independent of effects on ruminal digestion
Authors: Faverdin P. , M’hamed D. , Vérité R. 
Published: May 2017

AbstractThe rôle of protein in food intake regulation is complex in ruminants. Previous research has shown that a deficiency in degradable nitrogen (N) could affect microbial activity and decrease intake. On the other hand, an increase in metabolizable protein content of the diet seems to stimulate food intake in lactating dairy cows. The aim of this experiment was to determine whether metabolizable protein supply plays a direct rôle in the stimulation of food intake. Treatments comprised two infusions of soya protein isolate (800 g/day) either into the rumen (RP) or into the duodenum (DP), which were compared with two iso-energy infusions of glucose (880 g/day) either into the rumen (RG) or into the duodenum (DG). Four ruminally and duodenally cannulated cows producing 36·5 kg/day of milk were assigned to a 4 ✕ 4 Latin-square design with periods of 4 weeks. Duodenal infusions of protein (DP) significantly increased (P < 0·05) dry-matter intake (DMI) ( +1·9 kg/day), rate of intake ( + 8·2 g DMI per min), milk yield ( + 4 kg/day), protein content ( + 2·3 g/kg) and protein yield ( +191 g/day) compared with the glucose infusion in the duodenum (DG). No significant effect was observed with ruminal infusion of protein (RP) compared with the glucose infusion in the rumen (RG). The protein infusions had no effect (P > 0·05) on the apparent digestibility of dry matter, organic matter, neutral-detergent fibre or acid-detergent fibre and also no or only small effects on ruminal fermentation variables. Plasma concentrations of most of the essential amino acids increased significantly with the duodenal infusion of protein, whereas ruminal infusion of protein had no significant effect. It is concluded that direct supply of metabolizable protein stimulates intake independently of ruminal digestion effects.
Animal breeding in the (post-) genomic era
Authors: Goddardt M. E. 
Published: May 2017

AbstractOne of the benefits of the genomics revolution for animal production will be knowledge of genes that can be used to select more profitable livestock. Although it is possible to use genetic markers linked to genes of economic importance, tests for the genes themselves will be much more successful. Consequently finding genes of economic importance to livestock will be a major research aim for the future. Most traits of economic importance are quantitative traits affected by many genes. Mutations at many genes (e.g. 500) and at many positions within a gene (e.g. 1000 coding and non-coding bases) can affect a typical quantitative trait. The effect of these mutations on phenotype is usually small (e.g. 0·1 standard deviation) but occasionally large. Many mutations are lost from the population through genetic drift and selection, so that polymorphisms exist at only a subset of the relevant genes (e.g. 100 genes). Finding these genes, that have relatively small effects, is more difficult than finding genes for a classical Mendellian trait but, as the genomic tools become more powerful, it is becoming feasible and some successes have already occurred. The standard approach is to map a quantitative trait loci (QTL) to a chromosome region using linkage and linkage disequilibrium. Then test polymorphisms in positional candidate genes for an effect on the trait. Tools such as genomic sequence, EST collections and comparative maps make this approach feasible. Candidate genes can be selected based on functional data such as gene expression obtained from microarrays. At present the gain in rate of genetic improvement from use of DNA-based tests for QTL is small, because selection without them is already quite accurate, not enough QTL have been identified and genotyping is too expensive. However, in the future, with many QTL identified and inexpensive genotyping combined with decreased generation intervals, large gains are possible.