bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling Doxorubicin-Induced Cardiotoxicity in Human Pluripotent Stem Cell Derived-Cardiomyocytes
Authors: Maillet Agnes , Tan Kim , Chai Xiaoran , Sadananda Singh N. , Mehta Ashish , Ooi Jolene , Hayden Michael R. , Pouladi Mahmoud A. , Ghosh Sujoy , Shim Winston , Brunham Liam R. 
Published: May 2016

AbstractDoxorubicin is a highly efficacious anti-cancer drug but causes cardiotoxicity in many patients. The mechanisms of doxorubicin-induced cardiotoxicity (DIC) remain incompletely understood. We investigated the characteristics and molecular mechanisms of DIC in human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). We found that doxorubicin causes dose-dependent increases in apoptotic and necrotic cell death, reactive oxygen species production, mitochondrial dysfunction and increased intracellular calcium concentration. We characterized genome-wide changes in gene expression caused by doxorubicin using RNA-seq, as well as electrophysiological abnormalities caused by doxorubicin with multi-electrode array technology. Finally, we show that CRISPR-Cas9-mediated disruption of TOP2B, a gene implicated in DIC in mouse studies, significantly reduces the sensitivity of hPSC-CMs to doxorubicin-induced double stranded DNA breaks and cell death. These data establish a human cellular model of DIC that recapitulates many of the cardinal features of this adverse drug reaction and could enable screening for protective agents against DIC as well as assessment of genetic variants involved in doxorubicin response.
Sorting quantum systems efficiently
Authors: Ionicioiu Radu 
Published: May 2016

AbstractMeasuring the state of a quantum system is a fundamental process in quantum mechanics and plays an essential role in quantum information and quantum technologies. One method to measure a quantum observable is to sort the system in different spatial modes according to the measured value, followed by single-particle detectors on each mode. Examples of quantum sorters are polarizing beam-splitters (PBS) – which direct photons according to their polarization – and Stern-Gerlach devices. Here we propose a general scheme to sort a quantum system according to the value of any d-dimensional degree of freedom, such as spin, orbital angular momentum (OAM), wavelength etc. Our scheme is universal, works at the single-particle level and has a theoretical efficiency of 100%. As an application we design an efficient OAM sorter consisting of a single multi-path interferometer which is suitable for a photonic chip implementation.
Improved Drain Current Saturation and Voltage Gain in Graphene–on–Silicon Field Effect Transistors
Authors: Song Seung Min , Bong Jae Hoon , Hwang Wan Sik , Cho Byung Jin 
Published: May 2016

AbstractGraphene devices for radio frequency (RF) applications are of great interest due to their excellent carrier mobility and saturation velocity. However, the insufficient current saturation in graphene field effect transistors (FETs) is a barrier preventing enhancements of the maximum oscillation frequency and voltage gain, both of which should be improved for RF transistors. Achieving a high output resistance is therefore a crucial step for graphene to be utilized in RF applications. In the present study, we report high output resistances and voltage gains in graphene-on-silicon (GoS) FETs. This is achieved by utilizing bare silicon as a supporting substrate without an insulating layer under the graphene. The GoSFETs exhibit a maximum output resistance of 2.5 MΩ∙μm, maximum intrinsic voltage gain of 28 dB, and maximum voltage gain of 9 dB. This method opens a new route to overcome the limitations of conventional graphene-on-insulator (GoI) FETs and subsequently brings graphene electronics closer to practical usage.
Matrix stiffness promotes cartilage endplate chondrocyte calcification in disc degeneration via miR-20a targeting ANKH expression
Authors: Liu Ming-Han , Sun Chao , Yao Yuan , Fan Xin , Liu Huan , Cui You-Hong , Bian Xiu-Wu , Huang Bo , Zhou Yue 
Published: May 2016

AbstractThe mechanical environment is crucial for intervertebral disc degeneration (IDD). However, the mechanisms underlying the regulation of cartilage endplate (CEP) calcification by altered matrix stiffness remain unclear. In this study, we found that matrix stiffness of CEP was positively correlated with the degree of IDD and stiff matrix, which mimicked the severe degeneration of CEP, promoted inorganic phosphate-induced calcification in CEP chondrocytes. Co-expression analysis of the miRNA and mRNA profiles showed that increasing stiffness resulted in up-regulation of miR-20a and down-regulation of decreased ankylosis protein homolog (ANKH) during inorganic phosphate-induced calcification in CEP chondrocytes. Through a dual luciferase reporter assay, we confirmed that miR-20a directly targets 3′-untranslated regions of ANKH. The inhibition of miR-20a attenuated the calcium deposition and calcification-related gene expression, whereas the overexpression of miR-20a enhanced calcification in CEP chondrocytes on stiff matrix. The rescue of ANKH expression restored the decreased pyrophosphate efflux and inhibited calcification. In clinical samples, the levels of ANKH expression were inversely associated with the degeneration degree of CEP. Thus, our findings demonstrate that the miR-20a/ANKH axis mediates the stiff matrix- promoted CEP calcification, suggesting that miR-20a and ANKH are potential targets in restraining the progression of IDD.
Transcription factors YY1, Sp1 and Sp3 modulate dystrophin Dp71 gene expression in hepatic cells
Authors: Peñuelas-Urquides Katia , Becerril-Esquivel Carolina , Mendoza-de-León Laura C. , Silva-Ramírez Beatriz , Dávila-Velderrain José , Cisneros Bulmaro , de León Mario Bermúdez 
Published: May 2016

Dystrophin Dp71, the smallest product encoded by the Duchenne muscular dystrophy gene, is ubiquitously expressed in all non-muscle cells. Although Dp71 is involved in various cellular processes, the mechanisms underlying its expression have been little studied. In hepatic cells, Dp71 expression is down-regulated by the xenobiotic β-naphthoflavone. However, the effectors of this regulation remain unknown. In the present study we aimed at identifying DNA elements and transcription factors involved in Dp71 expression in hepatic cells. Relevant DNA elements on the Dp71 promoter were identified by comparing Dp71 5′-end flanking regions between species. The functionality of these elements was demonstrated by site-directed mutagenesis. Using EMSAs and ChIP, we showed that the Sp1 (specificity protein 1), Sp3 (specificity protein 3) and YY1 (Yin and Yang 1) transcription factors bind to the Dp71 promoter region. Knockdown of Sp1, Sp3 and YY1 in hepatic cells increased endogenous Dp71 expression, but reduced Dp71 promoter activity. In summary, Dp71 expression in hepatic cells is carried out, in part, by YY1-, Sp1- and Sp3-mediated transcription from the Dp71 promoter.
Clinical and Quality of Life Outcomes of Speech Treatment for Parkinson's Disease Delivered to the Home Via Telerehabilitation: A Noninferiority Randomized Controlled Trial
Authors: Theodoros Deborah G. , Hill Anne J. , Russell Trevor G. 
Published: May 2016

PurposeThis study investigated the noninferiority and validity of an intensive speech treatment for people with Parkinson's disease (PD) delivered via telerehabilitation to the home. The effect of location on online delivery was also investigated.MethodIn this single-blinded, randomized controlled noninferiority trial, 31 participants with dysarthria associated with PD from a metropolitan area were randomly assigned to either face-to-face or online Lee Silverman Voice Treatment (LSVT LOUD). A cohort of 21 participants from nonmetropolitan areas was also recruited and treated online. Outcomes were assessed using acoustic, perceptual, and quality of life measures.ResultsNoninferiority of online treatment was confirmed through comparable clinical and quality of life outcomes for the metropolitan online and face-to-face treatment groups. Significant improvements posttreatment were achieved for several acoustic, perceptual, and quality of life measures across the groups. No significant effect of online treatment location was identified.ConclusionsClinical and quality of life outcomes supported the noninferiority and validity of online delivery of intensive speech treatment to people with PD in the home. Future research should address the implementation of online treatment in a clinical service, cost analyses, and potentially technology-enabled clinical pathways for people with PD in order to maintain optimal communication and quality of life.
MicroRNA biosensing through spectral interferometry‐based surface plasmon resonance using telecommunication wavelength
Authors: Hsu S.‐H. , Lin C.‐Y. , Tuan C.‐T. , Chang C.‐C. 
Published: May 2016

The polarisation maintaining (PM) fibre constructed the spectral interferometry‐based surface plasmon resonance to detect the very small molecule micro ribonucleic acid (miRNA) with a big biological impact on cancer diagnosis. Other than the linear polariser and birefringence crystal in the input of the traditional spectral interferometry, the polarisation controller was simply placed on the injected light to control the transverse electric and transverse magnetic polarised magnitudes through the stable polarisation state of PM fibres for distinct dynamic‐range fringes on the optical spectrum analyser (OSA). Owing to the high precision and stability control of operating wavelengths in optical fibre communications, the low‐coherence telecommunication source was utilised to detect 21‐mer deoxyribonucleic acid which changed U to T according the miRNA‐21 sequence. The sensitivity with probing was showing 0.065 nm/(μg/ml), which implied the resolution as 0.3 μg/ml under the 0.02 nm wavelength accuracy on OSA.
B-Type Cardiac Natriuretic Peptides in the Neonatal and Pediatric Intensive Care Units
Authors: Cantinotti Massimiliano 
Published: May 2016

AbstractDuring the last decade, interest in the brain natriuretic peptide (BNP) and N-terminal probrain natriuretic peptide (NT-proBNP) in the pediatric population has progressively increased. The aim of this article is to provide an up to date review of evidences regarding the use of BNP/NT-proBNP in pediatrics, with a particular focus on neonatal intensive care and congenital heart disease. The potentialities of the BNP have been demonstrated in multiple settings, particularly: the screening of congenital/acquired heart disease (CHD) versus pulmonary disease; the evaluation of CHD severity (grade of heart failure, degree of left-to-right shunts); the management of children undergoing cardiac surgery; and monitoring premature infants with patent arterial duct. BNP/NT-proBNP values may be considered an easy and relatively low cost additional diagnostic and prognostic tool. Interpretation of BNP values in children requires attention to important factors, including: laboratory methods, the type of cardiac defect, its severity, and the presence of extracardiac conditions. Of these, the hemodynamic characteristic of CHD and physiologic variations of BNP values occurring during the first weeks of life play a major role. The current evidences in favor of BNP use are mainly derived from single-center, nonrandomized studies, and cost-effectiveness analysis are still lacking. As such, despite sufficient evidences supporting the diagnostic and prognostic potentialities of BNP, these findings should be reinforced by multicenter, randomized studies specifically designed to evaluate outcomes and cost-effectiveness. In addition, standard consensus documents/guidelines, that are currently lacking, are warranted for a more systematic use of BNP in the pediatric age.
Glycolipid GD3 and GD3 synthase are key drivers for glioblastoma stem cells and tumorigenicity
Authors: Yeh Shih-Chi , Wang Pao-Yuan , Lou Yi-Wei , Khoo Kay-Hooi , Hsiao Michael , Hsu Tsui-Ling , Wong Chi-Huey 
Published: May 2016

Significance
Glioblastoma multiforme (GBM) is the most malignant brain tumor. The recurrence or chemoresistance of GBM is attributed to the presence of cancer stem cells (CSCs). Although many studies indicate that CD133 protein is a biomarker for GBM CSC (GSC) enrichment, CD133 is not specific for GSCs and is also present on cancer cells. In this study, we report that ganglioside D3 (GD3) is not only an alternative marker but also an additional marker to CD133 for the identification and enrichment of GSCs. We further prove that the properties of CSCs in GBM are suppressed when GD3 synthase is inhibited, supporting GD3 as a GBM stem cell marker and a promising therapeutic target for GBM treatment.
Measuring Feelings of Staff Members Towards Their Most Complex Female and Male Forensic Psychiatric Patients: A Pilot Study into Gender Differences
Authors: de Vogel Vivienne , Louppen Marijke 
Published: May 2016

It has been suggested that female forensic psychiatric patients evoke different or stronger feelings in their treatment staff compared to male forensic patients. More specifically, it has been stated that it can be more difficult and emotionally draining to work with women as they are seen as more manipulative and demanding than men (Lewis, 2006 ). However, there is not much empirical research to support this suggestion. In this pilot study, 146 staff members working in a gender-mixed forensic psychiatric hospital filled out the Feeling Word Checklist 30-item version for their most complex, demanding, female and male patient. Overall, it was found that staff members felt more helpful, accepting, strong, relaxed, affectionate, sympathetic, and receptive towards their most complex female forensic patient and more anxious, threatened and overwhelmed by their most complex male forensic patient. Differences were found between more experienced and less experienced staff members as well as between female and male staff members. Staff members working over 5 years in this hospital experienced much fewer differences in feelings towards female and male patients. Less experienced staff had significantly more positive feelings towards female patients and more negative feelings towards male patients. Female staff members usually felt more strong and in control with female patients and more overwhelmed by male patients. Male staff members felt more angry with male patients and more receptive towards female patients. Compared to female staff members, male staff members felt more cautious and embarrassed with female patients. It was concluded that there are substantial differences in feelings of staff members towards female and male forensic patients, with staff reporting predominantly more positive feelings towards women. More training, intervision, and support with respect to working in forensic gender-mixed settings would be valuable, especially for mental health professionals who are early in their career. Finally, suggestions are provided for future research into this topic.
EMC3-EIRENE modelling of edge impurity transport in the stochastic layer of the large helical device compared with extreme ultraviolet emission measurements
Authors: Dai Shuyu , Kobayashi M. , Kawamura G. , Morita S. , Zhang H.M. , Oishi T. , Feng Y. , Wang D.Z. , Suzuki Y. 
Published: May 2016

Abstract
The transport properties and line emissions of carbon impurity in the stochastic layer of the Large Helical Device have been investigated with the 3D edge transport code EMC3-EIRENE. A parameter study has been performed to examine the sensitivity of the simulation results on each transport term in the impurity transport model and the impurity source characteristics, i.e. the source amount and the location. The modelling has revealed that in order to reproduce the experimental results of the emission distribution, the impurity perpendicular transport coefficient (D
imp) and the first wall source play important roles, while changes to the ion thermal and the friction forces are rather irrelevant. The detailed study of flux tube tracing and magnetic field structure in the edge stochastic layer, in relation to impurity transport, has shown that the deeper penetration of impurity into the higher plasma density region due to the enhanced D
imp and the first wall source is responsible for the change of emission pattern as well as the intensity. The analysis indicates that D
imp might be larger than that of background plasma by a few factors and also that there probably exists a substantial amount of first wall impurity source.
A 6% measurement of the Hubble parameter at z∼0.45: direct evidence of the epoch of cosmic re-acceleration
Authors: Moresco Michele , Pozzetti Lucia , Cimatti Andrea , Jimenez Raul , Maraston Claudia , Verde Licia , Thomas Daniel , Citro Annalisa , Tojeiro Rita , Wilkinson David 
Published: May 2016

Deriving the expansion history of the Universe is a major goal of modern cosmology. To date, the most accurate measurements have been obtained with Type Ia Supernovae (SNe) and Baryon Acoustic Oscillations (BAO), providing evidence for the existence of a transition epoch at which the expansion rate changes from decelerated to accelerated. However, these results have been obtained within the framework of specific cosmological models that must be implicitly or explicitly assumed in the measurement. It is therefore crucial to obtain measurements of the accelerated expansion of the Universe independently of assumptions on cosmological models. Here we exploit the unprecedented statistics provided by the Baryon Oscillation Spectroscopic Survey (BOSS, [1-3]) Data Release 9 to provide new constraints on the Hubble parameterH(z) using thecosmic chronometersapproach. We extract a sample of more than 130000 of the most massive and passively evolving galaxies, obtaining five new cosmology-independentH(z) measurements in the redshift range 0.3 < z < 0.5, with an accuracy of ∼11–16% incorporating both statistical and systematic errors. Once combined, these measurements yield a 6% accuracy constraint ofH(z = 0.4293) = 91.8 ± 5.3 km/s/Mpc. The new data are crucial to provide the first cosmology-independent determination of the transition redshift at high statistical significance, measuring zt = 0.4 ± 0.1, and to significantly disfavor the null hypothesis of no transition between decelerated and accelerated expansion at 99.9% confidence level. This analysis highlights the wide potential of the cosmic chronometers approach: it permits to derive constraints on the expansion history of the Universe with results competitive with standard probes, and most importantly, being the estimates independent of the cosmological model, it can constrain cosmologies beyond—and including—the ΛCDM model.
BAM-matcher: a tool for rapid NGS sample matching
Authors: Wang Paul P.S. , Parker Wendy T. , Branford Susan , Schreiber Andreas W. 
Published: May 2016

Abstract
The standard method used by high-throughput genome sequencing facilities for detecting mislabelled samples is to use independently generated high-density SNP data to determine sample identity. However, as it has now become commonplace to have multiple samples sequenced from the same source, such as for analysis of somatic variants using matched tumour and normal samples, we can directly use the genotype information inherent in the sequence data to match samples and thus bypass the need for additional laboratory testing. Here we present BAM-matcher, a tool that can rapidly determine whether two BAM files represent samples from the same biological source by comparing their genotypes. BAM-matcher is designed to be simple to use, provides easily interpretable results, and is suitable for deployment at early stages of data processing pipelines.
Availability and implementation: BAM-matcher is licensed under the Creative Commons by Attribution license, and is available from: https://bitbucket.org/sacgf/bam-matcher .
Supplementary information: Supplementary data are available at Bioinformatics online.
Contact: paul.wang@sa.gov.au
A Career Exploration Program: An Effective Alternative to the Traditional Use of Faculty Advisors
Authors: Olive Kenneth E. , Kwasigroch Thomas E. , Wooten Daniel J. , Lybrand Cynthia , Peeples Catherine R. 
Published: May 2016

Problem
Providing medical students with resources to make effective career choices is challenging for medical schools as career options outnumber the formal clinical rotations students can experience during their undergraduate education.

Approach
In 2009, the authors introduced the Career Exploration (CE) courses into the required curriculum at the Quillen College of Medicine. This three-course sequence includes large-group sessions addressing broad issues related to career choices, small-group specialty interest groups, individual student self-assessments, assignments through which students receive individualized feedback, and individual student advising sessions. The overall objective of the course sequence is to involve all students in career planning from the beginning of medical school so as to help them make more informed career decisions.

Outcomes
The authors used improvement in student satisfaction with career planning activities as a surrogate measure for the outcome of helping students make more informed career choices. Students evaluated the CE courses positively, and overall satisfaction scores averaged 4 (1 = poor to 5 = excellent). Scores on Association of American Medical Colleges Graduation Questionnaire items related to career planning showed improved student satisfaction from 2010 to 2015.

Next Steps
Succession planning for the first- and second-year career advisor is vital—as is faculty development for all clinical advisors to ensure that they have current information regarding both the curriculum and Match process, especially as residency selection becomes increasingly competitive. Enhancing the role of fourth-year students who serve as CE III mentors has the potential to prepare these students to be better teachers as residents.