bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Meteorological Drought in a Changing Environment: An Example in the Upper Yangtze River
Authors: Shen Jiaju , Yang Hanbo , Liu Ziwei , Li Changming , Li Sien , Cui Yaokui , Yang Dawen 
Published: October 2024

AbstractRecent studies have suggested that drought projections using Palmer drought severity index (PDSI) and standardized evapotranspiration precipitation index (SPEI) may overestimate drought severity. This overestimation occurs because the potential evapotranspiration (PET) calculations fail to consider the interactive effects of vegetation responses such as increased leaf area index (LAI) and constrained stomatal conductance, which are influenced by elevated atmospheric CO2 concentrations ([CO2]). To address this issue, our study replaced the traditional Penman‐Monteith (PM) equation with a recently proposed PET equation that includes the effects of changing [CO2] and LAI to assess droughts at monthly scale in the Upper Yangtze River basin, which experiences the vegetation greening. The findings indicated a consistent increasing trend in drought conditions with minimal discrepancy between the two equations over the historical period (1986–2017). This consistency arises because the water‐saving effects of increased [CO2] and the greening effects of rising LAI largely counterbalance each other. However, for the future period (2018–2100), projections using PM equation predicted an intensification of drought conditions. In contrast, the improved SPEI indicated no significant drought variations, and the improved PDSI suggested a wetting trend. This divergence can be attributed to the water‐saving effects increasingly outweighing the greening effects, as PET shows a decreasing sensitivity to LAI with LAI increasing, but maintains a near‐constant sensitivity to elevated [CO2]. Consequently, the indices based on PM equation tend to overestimate future drought severity. Overall, this study demonstrates that the new PET estimation method is more capable of responding to the changing environment.
AniMAIRE‐A New Openly Available Tool for Calculating Atmospheric Ionising Radiation Dose Rates and Single Event Effects During Anisotropic Conditions
Authors: Davis C. S. W. , Baird F. , Lei F. , Ryden K. , Dyer C. 
Published: October 2024

AbstractAniMAIRE (Anisotropic Model for Atmospheric Ionising Radiation Effects) is a new model and Python toolkit for calculating radiation dose rates experienced by aircraft during anisotropic solar energetic particle events. AniMAIRE expands the physics of the MAIRE + model such that dose rate calculations can be performed for anisotropic solar energetic particle conditions by supplying a proton or alpha particle rigidity spectrum, a pitch angle distribution, and the conditions of Earth's magnetosphere. In this paper, we describe the algorithm and top‐level structure of AniMAIRE and showcase AniMAIRE's capabilities by analyzing the dose rate maps that AniMAIRE produces when the time‐dependent spectra and pitch angle distribution for Ground Level Enhancement (GLE) 71 are input. We find that the dose rates AniMAIRE produces for the event fall between the dose rates produced by the WASAVIES and CRAC:DOMO models. Dose rate maps that evolve throughout the event are also shown, and it is found that each peak in the input pitch angle distribution generates a dose rate hotspot in each of the polar regions. AniMAIRE has been made available openly online so that it can be downloaded and run freely on local machines and so that the space weather community can easily contribute to it using Github forking.
Forecasting Lakes' Chlorophyll Concentrations Using Satellite Images and Generative Adversarial Networks
Authors: Nagkoulis Nikolaos , Vasiloudis Giorgos , Moumtzidou Anastasia , Gialampoukidis Ilias , Vrochidis Stefanos , Kompatsiaris Ioannis 
Published: October 2024

AbstractSatellite data are extensively used for water quality monitoring purposes, offering a significantly reduced cost compared to in situ data sampling. Using past measurements to predict future conditions remains a challenging task, because of the complexity of the natural phenomena that are involved, with great potential in terms of water resources management. This paper proposes a model that can be used to forecast Chlorophyll‐ (Chl‐) values in water bodies, which are a common water quality indicator. The operation of the model lays on the fact that typically Chl‐ increases and decreases periodically. First, we apply C2RCC, which is a common atmospheric correction algorithm, to Sentinel‐2 images to get Chl‐ maps for 15 lakes for 12 consecutive months around Europe. Then, we use this data set (1,000 Sentinel‐2 images) to train a Generative Adversarial Network (GAN) to recognize spatiotemporal patterns. To accomplish this task, pix2pix algorithm is employed, matching consecutive past and current Chl‐ maps to future Chl‐ maps. This model has been applied to 3 water bodies around Europe that are not included in the 15‐lakes training data set and has been found to perform accurately, achieving high Pearson and Spearman correlations and low RMSE values. Overall, the model can be used to make Chl‐ maps' predictions with low computational cost and without using any in situ data and without the requirement of training for every water body.
Periostin+ myeloid cells improved long bone regeneration in a mechanosensitive manner
Authors: Wang Ziyan , Lin Minmin , Pan Yonghao , Liu Yang , Yang Chengyu , Wu Jianqun , Wang Yan , Yan Bingtong , Zhou Jingjing , Chen Rouxi , Liu Chao 
Published: October 2024

AbstractMyeloid cells are pivotal in the inflammatory and remodeling phases of fracture repair. Here, we investigate the effect of periostin expressed by myeloid cells on bone regeneration in a monocortical tibial defect (MTD) model. In this study, we show that periostin is expressed by periosteal myeloid cells, primarily the M2 macrophages during bone regeneration. Knockout of periostin in myeloid cells reduces cortical bone thickness, disrupts trabecular bone connectivity, impairs repair impairment, and hinders M2 macrophage polarization. Mechanical stimulation is a regulator of periostin in macrophages. By activating transforming growth factor-β (TGF-β), it increases periostin expression in macrophages and induces M2 polarization. This mechanosensitive effect also reverses the delayed bone repair induced by periostin deficiency in myeloid cells by strengthening the angiogenesis-osteogenesis coupling. In addition, transplantation of mechanically conditioned macrophages into the periosteum over a bone defect results in substantially enhanced repair, confirming the critical role of macrophage-secreted periostin in bone repair. In summary, our findings suggest that mechanical stimulation regulates periostin expression and promotes M2 macrophage polarization, highlighting the potential of mechanically conditioned macrophages as a therapeutic strategy for enhancing bone repair.
Versatility of 14-3-3 proteins and their roles in bone and joint-related diseases
Authors: Zhou Renpeng , Hu Weirong , Ma Peter X. , Liu Chuan-ju 
Published: October 2024

AbstractBone and joint-related diseases, including osteoarthritis (OA), rheumatoid arthritis (RA), and bone tumors, pose significant health challenges due to their debilitating effects on the musculoskeletal system. 14-3-3 proteins, a family of conserved regulatory molecules, play a critical role in the pathology of these diseases. This review discusses the intricate structure and multifunctionality of 14-3-3 proteins, their regulation of signaling pathways, and their interactions with other proteins. We underscore the significance of 14-3-3 proteins in the regulation of osteoblasts, osteoclasts, chondrocytes, and bone remodeling, all key factors in the maintenance and dysfunction of bone and joint systems. Specific focus is directed toward elucidating the contribution of 14-3-3 proteins in the pathology of OA, RA, and bone malignancies, where dysregulated 14-3-3-mediated signaling cascades have been implicated in the disease processes. This review illuminates how the perturbation of 14-3-3 protein interactions can lead to the pathological manifestations observed in these disorders, including joint destruction and osteolytic activity. We highlight cutting-edge research that positions 14-3-3 proteins as potential biomarkers for disease progression and as innovative therapeutic targets, offering new avenues for disease intervention and management.
Animal life in the shallow subseafloor crust at deep-sea hydrothermal vents
Authors: Bright Monika , Gollner Sabine , de Oliveira André Luiz , Espada-Hinojosa Salvador , Fulford Avery , Hughes Ian Vincent , Hourdez Stephane , Karthäuser Clarissa , Kolar Ingrid , Krause Nicole , Le Layec Victor , Makovec Tihomir , Messora Alessandro , Mitchell Jessica , Pröts Philipp , Rodríguez-Ramírez Ivonne , Sieler Fanny , Sievert Stefan M. , Steger Jan , Tinta Tinkara , Winter Teresa Rosa Maria , Bright Zach , Coffield Russel , Hill Carl , Ingram Kris , Paris Alex 
Published: October 2024

AbstractIt was once believed that only microbes and viruses inhabited the subseafloor crust beneath hydrothermal vents. Yet, on the seafloor, animals like the giant tubeworm Riftia pachyptila thrive. Their larvae are thought to disperse in the water column, despite never being observed there. We hypothesized that these larvae travel through the subseafloor via vent fluids. In our exploration, lifting lobate lava shelves revealed adult tubeworms and other vent animals in subseafloor cavities. The discovery of vent endemic animals below the visible seafloor shows that the seafloor and subseafloor faunal communities are connected. The presence of adult tubeworms suggests larval dispersal through the recharge zone of the hydrothermal circulation system. Given that many of these animals are host to dense bacterial communities that oxidize reduced chemicals and fix carbon, the extension of animal habitats into the subseafloor has implications for local and regional geochemical flux measurements. These findings underscore the need for protecting vents, as the extent of these habitats has yet to be fully ascertained.
Identification of Shemin pathway genes for tetrapyrrole biosynthesis in bacteriophage sequences from aquatic environments
Authors: Wegner Helen , Roitman Sheila , Kupczok Anne , Braun Vanessa , Woodhouse Jason Nicholas , Grossart Hans-Peter , Zehner Susanne , Béjà Oded , Frankenberg-Dinkel Nicole 
Published: October 2024

AbstractTetrapyrroles such as heme, chlorophyll, and vitamin B12 are essential for various metabolic pathways. They derive from 5-aminolevulinic acid (5-ALA), which can be synthesized by a single enzyme (5-ALA synthase or AlaS, Shemin pathway) or by a two-enzyme pathway. The genomes of some bacteriophages from aquatic environments carry various tetrapyrrole biosynthesis genes. Here, we analyze available metagenomic datasets and identify alaS homologs (viral alaS, or valaS) in sequences corresponding to marine and freshwater phages. The genes are found individually or as part of complete or truncated three-gene loci encoding heme-catabolizing enzymes. Amino-acid sequence alignments and three-dimensional structure prediction support that the valaS sequences likely encode functional enzymes. Indeed, we demonstrate that is the case for a freshwater phage valaS sequence, as it can complement an Escherichia coli 5-ALA auxotroph, and an E. coli strain overexpressing the gene converts the typical AlaS substrates glycine and succinyl-CoA into 5-ALA. Thus, our work identifies valaS as an auxiliary metabolic gene in phage sequences from aquatic environments, further supporting the importance of tetrapyrrole metabolism in bacteriophage biology.
Antimicrobial and antibiotic-potentiating effect of calcium peroxide nanoparticles on oral bacterial biofilms
Authors: Bankar Neha , Latta Lorenz , Loretz Brigitta , Reda Bashar , Dudek Johanna , Hähl Hendrik , Hannig Matthias , Lehr Claus-Michael 
Published: October 2024

AbstractBacterial biofilms represent a prominent biological barrier against physical and chemical attacks. Disturbing the anaerobic microenvironment within biofilms by co-delivery of oxygen appears as a promising strategy to enhance the activity of an antibiotic. Here, we report the effect of oxygen-producing calcium peroxide nanoparticles (CaO2 NP) in combination with tobramycin sulfate (Tob). On Pseudomonas aeruginosa PAO1 biofilms in vitro, the additive effect of CaO2 NP towards Tob activity enhanced biofilm eradication by 2 log compared to Tob alone. For natural biofilms grown in the oral cavity of human volunteers in situ, treatment by CaO2 NP alone slightly increased the fraction of dead bacteria from 44% in various controls, including Tob alone, to 57%. However, the combination of CaO2 NP with Tob further increased the fraction of dead bacteria to 69%. These data confirm the intrinsic antimicrobial and antibiotic-potentiating effect of CaO2 NP also in a clinically relevant setting.
Dairy cows inoculated with highly pathogenic avian influenza virus H5N1
Authors: Baker Amy L. , Arruda Bailey , Palmer Mitchell V. , Boggiatto Paola , Sarlo Davila Kaitlyn , Buckley Alexandra , Ciacci Zanella Giovana , Snyder Celeste A. , Anderson Tavis K. , Hutter Carl R. , Nguyen Thao-Quyen , Markin Alexey , Lantz Kristina , Posey Erin A. , Kim Torchetti Mia , Robbe-Austerman Suelee , Magstadt Drew R. , Gorden Patrick J. 
Published: October 2024

Abstract
Highly pathogenic avian influenza (HPAI) H5N1 haemagglutinin clade 2.3.4.4b was detected in the USA in 2021. These HPAI viruses caused mortality events in poultry, wild birds and wild mammals. On 25 March 2024, HPAI H5N1 clade 2.3.4.4b was confirmed in a dairy cow in Texas in response to a multistate investigation into milk production losses1. More than 200 positive herds were identified in 14 US states. The case description included reduced feed intake and rumen motility in lactating cows, decreased milk production and thick yellow milk2,3. The diagnostic investigation revealed viral RNA in milk and alveolar epithelial degeneration and necrosis and positive immunoreactivity of glandular epithelium in mammary tissue. A single transmission event, probably from birds, was followed by limited local transmission and onward horizontal transmission of H5N1 clade 2.3.4.4b genotype B3.13 (ref. 4). Here we sought to experimentally reproduce infection with genotype B3.13 in Holstein yearling heifers and lactating cows. Heifers were inoculated by an aerosol respiratory route and cows by an intramammary route. Clinical disease was mild in heifers, but infection was confirmed by virus detection, lesions and seroconversion. Clinical disease in lactating cows included decreased rumen motility, changes to milk appearance and production losses. Infection was confirmed by high levels of viral RNA detected in milk, virus isolation, lesions in mammary tissue and seroconversion. This study provides the foundation to investigate additional routes of infection, pathogenesis, transmission and intervention strategies.
The individual and ecological characteristics of parental COVID-19 vaccination decisions
Authors: Hörnig Lukas , Schaffner Sandra , Schmitz Hendrik 
Published: October 2024

AbstractVaccination is a highly effective method to prevent the spread of COVID-19 and mitigate severe disease. In Germany, adult vaccination rates are relatively high at 85.5%, but rates are significantly lower for adolescents (69.6%) and children (20.0%). This discrepancy indicates that not all vaccinated parents choose to vaccinate their children. Analyzing data from a January 2022 online survey of 1,819 parents with children and adolescents, we explore the socio-economic factors influencing parents’ willingness to vaccinate themselves and their children. Our results show that individuals who vote for either side of the political extremes are less likely to vaccinate their children. This pattern is particularly strong for voters on the far right. In addition, we find that better educated parents are more likely to vaccinate both themselves and their children. Parents who vaccinate both themselves and their children demonstrate greater confidence in the vaccine’s effectiveness, while those who vaccinate only themselves are often motivated by a desire to regain personal freedoms. These insights highlight the need for targeted public health strategies to address specific concerns and improve vaccination rates among children and adolescents.
Proteo-genomic analyses in relatively lean Chinese adults identify proteins and pathways that affect general and central adiposity levels
Authors: Iona Andri , Yao Pang , Pozarickij Alfred , Kartsonaki Christiana , Said Saredo , Wright Neil , Lin Kuang , Millwood Iona , Fry Hannah , Mazidi Mohsen , Wang Baihan , Chen Yiping , Du Huaidong , Yang Ling , Avery Daniel , Schmidt Dan , Sun Dianjianyi , Pei Pei , Lv Jun , Yu Canqing , Hill Michael , Chen Junshi , Bragg Fiona , Bennett Derrick , Walters Robin , Li Liming , Clarke Robert , Chen Zhengming ,   China Kadoorie Biobank Collaborative Group
Published: October 2024

AbstractAdiposity is an established risk factor for multiple diseases, but the causal relationships of different adiposity types with circulating protein biomarkers have not been systematically investigated. We examine the causal associations of general and central adiposity with 2923 plasma proteins among 3977 Chinese adults (mean BMI = 23.9 kg/m²). Genetically-predicted body mass index (BMI), body fat percentage (BF%), waist circumference (WC), and waist-to-hip ratio (WHR) are significantly (FDR < 0.05) associated with 399, 239, 436, and 283 proteins, respectively, with 80 proteins associated with all four and 275 with only one adiposity trait. WHR is associated with the most proteins (n = 90) after adjusting for other adiposity traits. These associations are largely replicated in Europeans (mean BMI = 27.4 kg/m²). Two-sample Mendelian randomisation (MR) analyses in East Asians using cis-protein quantitative trait locus (cis-pQTLs) identified in GWAS find 30/2 proteins significantly affect levels of BMI/WC, respectively, with 10 showing evidence of colocalisation, and seven (inter-alpha-trypsin inhibitor heavy chain H3, complement factor B, EGF-containing fibulin-like extracellular matrix protein 1, thioredoxin domain-containing protein 15, alpha-2-antiplasmin, fibronectin, mimecan) are replicated in separate MR using different cis-pQTLs identified in Europeans. These findings identified potential novel mechanisms and targets, to our knowledge, for improved treatment and prevention of obesity and associated diseases.
Functional MRI of imprinting memory in awake newborn domestic chicks
Authors: Behroozi Mehdi , Lorenzi Elena , Tabrik Sepideh , Tegenthoff Martin , Gozzi Alessandro , Güntürkün Onur , Vallortigara Giorgio 
Published: October 2024

AbstractFilial imprinting, a crucial ethological paradigm, provides insights into the neurobiology of early learning and its long-term impact on behaviour. To date, invasive techniques like autoradiography or lesions have been used to study it, limiting the exploration of whole brain networks. Recent advances in fMRI for avian brains now open new windows to explore bird’s brain functions at the network level. We developed an fMRI technique for awake, newly hatched chicks, capturing BOLD signal changes during imprinting experiments. While early memory acquisition phases are understood, long-term storage and retrieval remain unclear. Our findings identified potential long-term storage of imprinting memories across a neural network, including the hippocampal formation, the medial striatum, the arcopallium, and the prefrontal-like nidopallium caudolaterale. This paradigm opens up new avenues for exploring the broader landscape of learning and memory in neonatal vertebrates, enhancing our understanding of behaviour and brain networks.
Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden
Authors: Westmeijer George , van Dam Femke , Kietäväinen Riikka , González-Rosales Carolina , Bertilsson Stefan , Drake Henrik , Dopson Mark 
Published: October 2024

AbstractThe continental bedrock contains groundwater-bearing fractures that are home to microbial populations that are vital in mediating the Earth’s biogeochemical cycles. However, their diversity is poorly understood due to the difficulty of obtaining samples from this environment. Here, a groundwater-bearing fracture at 975 m depth was isolated by employing packers in order to characterize the microbial community via metagenomes combined with prokaryotic and eukaryotic marker genes (16S and 18S ribosomal RNA gene). Genome-resolved analyses revealed a community dominated by sulfate-reducing Bacillota, predominantly represented by Candidatus Desulforudis audaxviator and with Wood-Ljungdahl as the most prevalent pathway for inorganic carbon fixation. Moreover, the eukaryotic community had a considerable diversity and was comprised of mainly flatworms, chlorophytes, crustaceans, ochrophytes, and fungi. These findings support the important role of the Bacillota, with the sulfate reducer Candidatus Desulforudis audaxviator as its main representative, as primary producers in the often energy-limited groundwaters of the continental subsurface.
A semi-transparent thermoelectric glazing nanogenerator with aluminium doped zinc oxide and copper iodide thin films
Authors: Al-Fartoos Mustafa Majid Rashak , Roy Anurag , Mallick Tapas K. , Tahir Asif Ali 
Published: October 2024

AbstractTo address the pressing need for reducing building energy consumption and combating climate change, thermoelectric glazing (TEGZ) presents a promising solution. This technology harnesses waste heat from buildings and converts it into electricity, while maintaining comfortable indoor temperatures. Here, we developed a TEGZ using cost-effective materials, specifically aluminium-doped zinc oxide (AZO) and copper iodide (CuI). Both AZO and CuI exhibit a high figure of merit (ZT), a key indicator of thermoelectric efficiency, with values of 1.37 and 0.72, respectively, at 340 K, demonstrating their strong potential for efficient heat-to-electricity conversion. Additionally, we fabricated an AZO-CuI based TEGZ prototype (5 × 5 cm²), incorporating eight nanogenerators, each producing 32 nW at 340 K. Early testing of the prototype showed a notable temperature differential of 22.5 °C between the outer and inner surfaces of the window glazing. These results suggest TEGZ could advance building energy efficiency, offering a futuristic approach to sustainable build environment.
Cryogenic quantum computer control signal generation using high-electron-mobility transistors
Authors: Ferraris Alberto , Cha Eunjung , Mueller Peter , Moselund Kirsten , Zota Cezar B. 
Published: October 2024

AbstractMultiplexed local charge storage, close to quantum processors at cryogenic temperatures could generate a multitude of control signals, for electronics or qubits, in an efficient manner. Such cryogenic electronics require generating quasi-static control signals with small area footprint, low noise, high stability, low power dissipation and, ideally, in a multiplexed fashion to reduce the number of input/outputs. In this work, we integrate capacitors with cryogenic high-electron mobility transistor (HEMT) arrays and demonstrate quasi-static bias generation using gate pulses controlled in time and frequency domains. Multi-channel bias generation is also demonstrated. Operation at 4 K exhibits improved bias signal variability and greatly reduced subthreshold swing, reaching values of ~6 mV/decade. Due to the very low threshold voltage of 80 mV at 4 K and the steep subthreshold swing, these circuits can provide an advantage over the silicon-based complementary metal-oxide-semiconductor equivalents by allowing operation at significantly reduced drive bias in the low output voltage regime <1 V. Together with their high-speed operation, this makes HEMTs an attractive platform for future cryogenic signal generation electronics in quantum computers.