bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irregular breakfast eating in type 2 diabetes mellitus is associated with greater social jetlag and poorer metabolic health
Authors: Mustafa Mohamad , Healy Ultan , Kosidialwa Oratile , Wong Matt , Alsalman Shayma , Conway Orla , Kelly Rachel M. , Sreenan Seamus , Coogan Andrew N. , McDermott John H. 
Published: October 2024

SummaryCircadian disruption, arising from conflict between internal circadian time and behavioural sleep–wake and fasting–feeding rhythms, may contribute to the prevalence of type 2 diabetes mellitus and disease severity. Previous studies have demonstrated a link between irregular breakfast eating and poorer metabolic health. We aimed to further explore the relationships between breakfast habits, circadian misalignment (social jetlag), and metabolic parameters in a cohort of adult participants with type 2 diabetes mellitus. A total of 330 adult participants with type 2 diabetes mellitus attending for routine clinical review completed structured questionnaires to assess habitual sleep timing, chronotype, and social jetlag. Statistical analysis was via inferential groupwise approaches and path analysis to establish interdependencies of effects of social jetlag, chronotype, and breakfast eating regularity on HbA1c. 22.7% of the participants reported eating breakfast five times or fewer a week, and were categorised as irregular breakfast eaters. Compared with those who ate breakfast six or seven times a week, irregular breakfast eaters had significantly higher HbA1c and diastolic blood pressure, were younger and had greater social jetlag. In the path analysis, irregular breakfast eating exerted a direct effect on HbA1c, whilst social jetlag exerted only an indirect effect on HbA1c through breakfast eating regularity. Chronotype did not exert any effect on HbA1c, but did exert an indirect effect on breakfast eating regularity via social jetlag. Our results showed that adult participants with type 2 diabetes mellitus, who ate breakfast irregularly had poorer metabolic health and greater social jetlag. The relationship between social jetlag and glycaemic control appears to be mediated through breakfast eating habits.
Identification of feline erythrocyte antigen 6 and lack of alloimmunization to feline erythrocyte antigen 4 in cats
Authors: Ternisien Thomas , Azoulay Florian , Bangash Mohammad Y. , Blais Marie‐Claude 
Published: October 2024

AbstractBackgroundNew feline erythrocyte antigens (FEAs) have been described based on the presence of naturally occurring alloantibodies (NOAb), but their immunogenicity and clinical relevance are poorly understood.Hypothesis/ObjectivesDescribe the immunogenicity of FEA 4 after sensitizing FEA 4–negative cats lacking NOAb and characterize anti–FEA 4 alloantibodies produced, including their rate of appearance, agglutination titer, and immunoglobulin class.AnimalsNineteen healthy type A cats were blood typed for FEAs 1 to 5 to identify suitable donor‐recipient pairs for FEA 4 sensitization.MethodsFour FEA 4–negative cats were transfused with FEA 4–positive red blood cells. Using a gel column technique, posttransfusion samples were screened daily for a week, weekly for a month, and monthly thereafter for anti–FEA 4 alloantibodies.ResultsAlloantibodies were not detected in the first 3 recipients despite repeated transfusions (1 and 3 additional transfusions for 2 and 1 recipients, respectively). In the 4th recipient, alloantibodies against its donor red blood cells were detected 21 days postsensitization. However, they were not directed against FEA 4, but rather against a novel FEA not yet described. The alloantibodies, named anti–FEA 6, remained detectable for >4 months after sensitization and were determined to be mostly immunoglobulin M based on sulfhydryl treatment.Conclusions and Clinical ImportanceFeline erythrocyte antigen 4 does not appear to be immunogenic because repeated sensitization of 4 cats failed to produce detectable anti–FEA 4 alloantibodies. A new immunogenic antigen, named FEA 6, has been discovered, but additional studies are needed to document its clinical importance.
The chemical language of plant–microbe–microbe associations: an introduction to a Virtual Issue
Authors: Hacquard Stéphane , Martin Francis M. 
Published: October 2024

This Editorial introduces the Virtual Issue ‘Chemical language of plant–microbe–microbe associations’ that includes the following papers: Basak et al. (2024), Böttner et al. (2023), Brisson et al. (2023), Feng et al. (2023), Gfeller et al. (2024), Gómez‐Pérez et al. (2023), Hong et al. (2022, 2023), Hu et al. (2024), Jiang et al. (2024), Lee et al. (2024), Nakano (2024), Ökmen et al. (2023), Revillini et al. (2023), Rovenich & Thomma (2023), Simonin et al. (2022), Snelders et al. (2023), Walsh et al. (2024), Wen et al. (2023), Xia et al. (2023), Xie et al. (2023), Zhang et al. (2023, 2024), Zheng et al. (2023), Zhou et al. (2023, 2024). Access the Virtual Issue at www.newphytologist.com/virtualissues.
The Unintended Consequence of Stringent Immigration Enforcement on Staffing Levels in Nursing Homes: Evidence from Secure Communities
Authors: Gunadi Christian 
Published: October 2024

AbstractThe provision of healthcare in the United States is increasingly reliant on immigrant workers. In this paper, I examine the impact of Secure Communities, a major immigration enforcement program designed to check the immigration status of all individuals arrested by local police, on staffing levels in nursing homes. Using a difference‐in‐differences strategy that exploits the staggered activation of Secure Communities across US counties, I found that the program reduced directcare staff hours per resident day by 0.073, an approximately 2% decline relative to the mean of treatment counties in the baseline period. This finding suggests that stringent immigration enforcement may exacerbate the healthcare worker shortage in the United States.
Recurrence rate of the odontogenic keratocysts patient cohort in a UK regional maxillofacial surgery unit
Authors: Gilvetti Ciro , Mahendran Krishantini , Gulati Aakshay , Barrett Andrew William 
Published: October 2024

AbstractBackgroundDespite the benign nature of odontogenic keratocyst (OKC), the reported recurrence rates range from 3% to 60% depending on the treatment modality. Surgical enucleation alone is often insufficient to prevent recurrence, thus adjunctive therapies have been adopted. The aim of this study was to determine the recurrence rate of OKC, both sporadic and those associated with naevoid basal cell carcinoma syndrome based on the treatment modality.Materials and MethodsTwo hundred patients diagnosed and treated between 2012 and 2021 for OKC were identified, from which 72 were excluded because of inadequate clinicopathological data. Forty‐four (34.3%) patients were cases of syndromic and 84 (65.5%) sporadic OKC.ResultsThe most common surgical procedure was enucleation alone (45%) followed by enucleation and removal of the associated tooth (28.1%). Carnoy's solution was the most used adjunctive method (14.7%). The overall recurrence rate of sporadic OKC in our study was 18.7% which was in line with the 16.6% reported by Al‐Moraissi et al. in their systematic review.ConclusionsSyndromic cases were statistically significantly more likely to recur at the same site of the surgery than sporadic cases (p < 0.05) with a recurrence rate of 52% (23 out of 44). Overall, adjunctive treatments to enucleation alone are associated with a lower recurrence rate but no one method eliminates recurrence.Practical ImplicationsIncisional biopsy and diagnosis of OKC can better tailor definitive treatment. Syndromic OKC is associated with a higher recurrence rate, and adjunctive treatments to enucleation alone help in reducing recurrence rate.
Forecasting Population in an Uncertain World: Approaches, New Uses, and Troubling Limitations
Authors: Lee Ronald 
Published: October 2024

AbstractThe long human lifespan enables long run forecasts of population size and age distribution. New methods include biodemographic research on upper limits to life expectancy and incorporation of early experiences affecting later life mortality such as smoking, obesity, and childhood health shocks. Some fertility forecasts incorporate education and quantum‐tempo insights. Statistical time series and Bayesian methods generate probabilistic forecasts. Yet recent decades have brought surprising changes in the economy, natural environment, and vital rates. In these changing circumstances we need new methods and the increasing use of probabilistic models and Bayesian methods incorporating outside information. The increasing use of microsimulation combined with aggregate forecasting methods is a very promising development enabling more detailed and heterogeneous forecasts. Some new uses of stochastic forecasts are interesting in themselves. Probabilistic mortality forecasts are used in finance and insurance, and a new Longevity Swap industry has been built on them. Random sample paths used to generate stochastic population forecasts can stress‐test public pension designs for fiscal stability and intergenerational equity. Population forecasting a few decades ago was a dull backwater of demographic research, but now it is increasingly important and is full of intellectual and technical challenges.
Unravelling the impact of cassava mosaic disease caused by Sri Lankan cassava mosaic virus infection: Insights from proteomics analysis of photosynthesis‐ and respiration‐related proteins
Authors: Vannatim Nattachai , Chaowongdee Somruthai , Malichan Srihunsa , Roytrakul Sittiruk , Charoenlappanit Sawanya , Siriwan Wanwisa 
Published: October 2024

AbstractSri Lankan cassava mosaic virus (SLCMV) poses a significant threat to global food security by affecting the production of cassava (Manihot esculenta) in South‐east Asia. It causes mosaic patterns on the leaves, disrupts photosynthesis and respiration, and alters carbohydrate accumulation in the tubers. This study aimed to identify proteins associated with cassava mosaic disease symptoms in three cassava cultivars: resistant (TMEB419), tolerant (Kasetsart 50, KU50) and susceptible (Rayong 11, R11) to SLCMV. These cultivars were inoculated with SLCMV using top‐cleft grafting and monitored at 21, 32 and 67 days post‐inoculation (dpi). The disease severity differed among the cultivars following SLCMV inoculation. Liquid chromatography–tandem mass spectrometry (LC–MS/MS)‐based proteomics analysis identified 541 differentially expressed proteins (DEPs), including photosynthesis‐ and respiration‐associated proteins such as RuBisCO large subunit‐binding protein α (LSBP), chlorophyll a‐b binding protein, photosystem I reaction centre subunit III (PSI‐F), glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and fructose‐bisphosphate aldolase (FBA). A protein–protein interaction network revealed interactions among PSI, PSII, cytochrome complex proteins and electron transporter proteins. Chlorophyll(ide) b reductase NYC1, upregulated in R11 (susceptible) at 21 and 32 dpi, interacted with chlorophyllase‐related proteins, suggesting that it has a role in altering chlorophyll metabolism. Reverse transcription‐quantitative PCR gene expression analysis confirmed upregulation of genes related to photosynthesis and respiration in KU50 and R11, predominantly at 21 or 32 dpi. These findings provide valuable insights into cassava responses to SLCMV infection, especially at the protein level and are key for developing resistant cultivars and effective disease management strategies in future research.
Improving reliability of PCR diagnostics for Xylophilus ampelinus by metagenome‐informed circumscription of the target taxon
Authors: Carminati Gaia , Bianchi Gian Luca , De Amicis Francesca , Cannistraci Isabella , Sadallah Abderraouf , Ermacora Paolo , Torelli Emanuela , Martini Marta , Firrao Giuseppe 
Published: October 2024

AbstractXylophilus ampelinus is a xylematic bacterium causing bacterial blight of grapevine, a disease regarded as a potential threat for viticulture in several countries. Currently, PCR detection is pivotal in diagnostic protocols due to the bacterium's infrequent occurrence in the field and the technical advantages of PCR. Recent metagenomic studies have unveiled diversity in its taxonomic domain, unknown when the most widely used assays for the detection of X. ampelinus infections were developed. In particular, PCR assays relying on highly conserved sequence regions, such as those surrounding ribosomal RNA genes, may be substituted with more specific PCR assays. In this study, we first investigated the diversity of detectable grapevine endophytes related to but different from X. ampelinus and delineated the genotaxonomic boundaries of the species in relation to the known (meta)genomes of closely related bacteria. Then, by exploiting the wealth of genomes now available for bacteria classified in the Burkholderiales, we devised several sets of primers targeting only X. ampelinus and its closest relatives. These primers were employed to (i) genotaxonomically circumscribe the grapevine endophyte species related to but distinguishable from X. ampelinus and (ii) develop a robust multiplex PCR assay expected to be specific for the species X. ampelinus based on in vitro and in silico evidence. The adoption of the multiplex PCR assay presented here is expected to reduce the risk of false positives in the diagnosis of bacterial blight of grapevine.
The prominent multiplication of Japanese soil‐borne wheat mosaic virus co‐infected with barley yellow mosaic virus in barley
Authors: Zhu Hongjing , Mishina Kohei , Tagiri Akemi , Chen Gang , Han Chenjing , Chen Ling , Nakamura Shingo , Kikuchi Shinji , Sassa Hidenori , Kato Tsuneo , Komatsuda Takao , Oono Youko 
Published: October 2024

AbstractVarious members of the viral genera Furovirus and Bymovirus are damaging pathogens of a range of crop species. Infection of the soil‐borne plasmodiophorid Polymyxa graminis transmits both Japanese soil‐borne wheat mosaic virus (JSBWMV) and the barley yellow mosaic virus (BaYMV) to barley, but their interaction during an episode of their co‐infection has not been characterized to date. Here, we present an analysis of the titer of JSBWMV and BaYMV in plants of winter barley growing over a five‐month period from late fall until mid‐spring. Although JSBWMV was detectable in the plants' roots four weeks earlier than BaYMV, the translocation of both viruses from the root to the leaves occurred nearly simultaneously. Both viruses were co‐localized in the roots, leaf sheathes, and leaf blades; however, in some stripes of leaf veins where infection by JSBWMV was prominent, BaYMV was not detectable. A substantial titer of both viruses persisted until early spring, after which JSBWMV became more prominent, being in a range of 10 to 100 times abundant of BaYMV. However, JSBWMV was only able to infect a single wheat accession (cv. Norin 61), whereas all of the wheat entries assayed appeared to be immune to BaYMV infection. Overall, our findings highlight the importance of resistance mechanisms against soil‐borne viruses in cereal crops, expanding our understanding of plant‐virus interactions and potentially informing strategies for crop protection against viral pathogens.
International review of blood donation screening for anti‐HBc and occult hepatitis B virus infection
Authors: Fu Michael X. , Faddy Helen M. , Candotti Daniel , Groves Jamel , Saa Paula , Styles Claire , Adesina Opeyemi , Carrillo Jose Perez , Seltsam Axel , Weber‐Schehl Marijke , O'Brien Sheila F. , Drews Steven J. , Aidoo Nana Benyin , Pajares Ángel Luis , Perez Laura Navarro , Deng Xuelian , van de Laar Thijs , Laperche Syria , Lehtisalo Riikka , Yilmaz Soner , Tsoi Wai‐Chiu , Juhl David , Niederhauser Christoph , Chenarsabz Nahid , O'Flaherty Niamh , Goto Naoko , Satake Masahiro , Renaud Christian , Lewin Antoine , Cloutier Marc , Sawadogo Salam , Reynolds Claire , Zhiburt Eugene , Muylaert An , Van Gaever Véronique , Garcia‐Otalora Michel‐Andres , Jarvis Lisa , Vermeulen Marion , Busch Michael , Blackmore Stuart , Jones Ann , Brailsford Su , Irving William L. , Andersson Monique , Simmonds Peter , Harvala Heli ,   the Virology subgroup of the ISBT WP‐TTID
Published: October 2024

AbstractBackgroundHepatitis B core antibody (anti‐HBc) screening has been implemented in many blood establishments to help prevent transmission of hepatitis B virus (HBV), including from donors with occult HBV infection (OBI). We review HBV screening algorithms across blood establishments globally and their potential effectiveness in reducing transmission risk.Materials and MethodsA questionnaire on HBV screening and follow‐up strategies was distributed to members of the International Society of Blood Transfusion working party on transfusion‐transmitted infectious diseases. Screening data from 2022 were assimilated and analyzed.ResultsA total of 30 unique responses were received from 25 countries. Sixteen respondents screened all donations for anti‐HBc, with 14 also screening all donations for HBV DNA. Anti‐HBc prevalence was 0.42% in all blood donors and 1.19% in new donors in low‐endemic countries; however, only 44% of respondents performed additional anti‐HBc testing to exclude false reactivity. 0.68% of anti‐HBc positive, HBsAg‐negative donors had detectable HBV DNA. Ten respondents did universal HBV DNA screening without anti‐HBc, whereas four respondents did not screen for either. Deferral strategies for anti‐HBc positive donors were highly variable. One transfusion‐transmission from an anti‐HBc negative donor was reported.DiscussionAnti‐HBc screening identifies donors with OBI but also results in the unnecessary deferral of a significant number of donors with resolved HBV infection and donors with false‐reactive anti‐HBc results. Whilst confirmation of anti‐HBc results could be improved to reduce donor deferral, transmission risks associated with anti‐HBc negative OBI donors must be considered. In high‐endemic areas, highly sensitive HBV DNA testing is required to identify infectious donors.
Real cameras, irreal things: Image‐making and ethnographic insight
Authors: Georgiev Konstantin 
Published: October 2024

AbstractThis article explores the effects of image‐making on ethnographic insight. The topic is approached within the epistemological framework of recent revisions of anthropology's reflexive turn. Empirical observation, photographic experiments, and the work of anthropologists, historians, and philosophers are employed to argue that photography—and, especially, experimental techniques—brings together different modes of knowledge and make communicable an array of irreal things. By working with data gathered serendipitously outside of the author's ethnographic project, the arguments of this article extend further and recover older theoretical assertions that ethnographic knowledge production involves the whole self.
Reconstruction of deep and perforating corneal defects in dogs—A review (Part II/III): Biomaterials and keratoprosthesis
Authors: Ledbetter Eric C. , Sanchez Rick F. , Repiso Marta Leiva 
Published: October 2024

AbstractThe surgical reconstruction of severe corneal ulcers is a common and crucial component of the clinical practice of veterinary ophthalmology. Numerous surgical techniques are used in dogs for corneal reconstruction, and these techniques may be categorized by the material used to repair the corneal lesion. The first part of the present review described procedures that utilize autogenous ocular tissues, homologous donor tissues, and heterologous donor tissues. In this second part of the review, the categories of biomaterials and keratoprosthetics will be summarized. Biomaterials that are reported for use in dogs include amniotic membrane, porcine urinary bladder acellular matrix, porcine small intestinal submucosa, acellular porcine corneal stroma, and other miscellaneous soft tissue and cartilage grafts (e.g., preserved equine renal capsule, autologous omentum, autologous buccal mucosa membrane, bovine pericardium, and homologous peritoneum). Descriptions of keratoprosthesis surgery in dogs are currently limited, but the use of artificial corneal transplants hold promise for dogs with severe, vision‐compromising corneal disease that is not amenable to other reconstruction techniques. This review describes the results of experimental studies evaluating these graft materials in dogs, and it will summarize the findings and outcomes of the clinical articles published in each material category. Reporting inconsistencies and areas where additional research is required will be highlighted to help guide future studies in this area. A major aim of this review is to help identify potential subjects that could be evaluated in future investigations and that might lead to refinements in clinical practice.
Effects of a bioengineered allogeneic cellular construct on burn‐related macrophage phenotype
Authors: Hernaez‐Estrada Beatriz , Steele Lindsay A. , Spiller Kara L. 
Published: October 2024

AbstractBioengineered allogeneic cellularised constructs (BACC) exert pro‐healing effects in burn wounds and skew macrophage phenotype towards a predominately reparative phenotype. However, whether BACC can modulate the phenotype of dysregulated macrophages, like those present in burn wounds, is not known. To better understand the macrophage modulatory characteristics of the BACC, primary human macrophages were polarised to the M2b phenotype, an immunosuppressive phenotype relevant to burn wounds, by simultaneously exposing macrophages to polystyrene plate‐coated immunoglobulin G and the endotoxin lipopolysaccharide (LPS). The resulting macrophage phenotype upregulated both inflammatory and reparative genes, and increased secretion of the M2b marker CCL1 compared to five different in vitro macrophage phenotypes. M2b macrophages were cultured with the BACC in the presence or absence of LPS to mimic infection, which is a common occurrence in burn wounds. The BACC caused up‐regulation of reparative gene sets and down‐regulation of pro‐inflammatory gene sets, even when LPS was present in the cell culture media. Co‐cultures were maintained for 1, 3, or 5 days in the presence of LPS, and by day 1 both non‐activated macrophages and M2b macrophages exhibited signs of endotoxin tolerance, as demonstrated by a reduced secretion of tumour necrosis factor α (TNFα) in response to fresh LPS stimulus. The BACC was not able to prevent endotoxin tolerance, but reparative genes were upregulated in macrophages chronically exposed to LPS. These results suggest that the BACC can promote a reparative phenotype in dysregulated macrophages relevant to the pathophysiology of burns.