bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and clinical impact of recreational drug consumption in people living with HIV on treatment: a cross-sectional study
Authors: Garin Noe , Zurita Beatriz , Velasco Cesar , Feliu Anna , Gutierrez Mar , Masip Montserrat , Mangues M Antonia 
Published: January 2017

ObjectivesDrug interactions, poor adherence to medication and high-risk sexual behaviour may occur in individuals with HIV using recreational drugs. Thus, we aimed to assess the prevalence of recreational drugs use and to explore its clinical impact in HIV patients on treatment.MethodsObservational, cross sectional, study conducted in a 700 bed university hospital, Barcelona, Spain. A total of 208 adults living with HIV on treatment were included. A questionnaire was administered by clinical pharmacists, including evaluation of sociodemographic variables, past 12-month drug consumption, adherence to antiretrovirals (Simplified Medication Adherence Questionnaire) and high-risk sexual behaviour (condomless sex/multiple partners). Additional data were obtained from clinical records. Recreational drug-antiretroviral interactions were checked in reference databases. Prevalence was calculated for 5% precision and 95% CI. Crude and adjusted binary logistic regressions were performed to identify associations between recreational drug use and adherence problems, and between recreational drug use and high-risk sexual behaviour.ResultsFrom the overall sample, 92 participants (44.2%) consumed recreational drugs over the past 1 year. Of these, 44 (48.8%) had used different types of recreational drugs in this period. We detected 11 recreational substances, including sildenafil and nitrites. The most consumed drugs were: cannabis (68.5%), cocaine (45.5%), nitrites (31.5%), sildenafil (28.3) and ecstasy (19.6%). Relevant interactions occurred in 46 (50%) of the individuals consuming drugs. Recreational drug consumption was found to be related to adherence problems with antiretrovirals (OR: 2.51 (95% CI 1.32 to 4.77) p=0.005) and high-risk sexual behaviour (OR: 2.81 (95% CI 1.47 to 5.39) p=0.002).ConclusionsRecreational drugs are frequently used by HIV patients on treatment. Classical drugs and new substances consumed in sexual context are usual. Recreational drug consumption interferes with several clinical outcomes, including potentially relevant interactions between drugs and antiretrovirals, adherence problems and high-risk sexual behaviour. Thus, there is the urgent need of implementing patient-centred care involving recreational drug consumption.
Clinical implications ofDNMT3Amutations in a Southeast Asian cohort of acute myeloid leukaemia patients
Authors: Tan Marcus , Ng Isaac K S , Chen Zhaojin , Ban Kenneth , Ng Christopher , Chiu Lily , Seah Elaine , Lin Mingxuan , Tai Bee Choo , Yan Benedict , Ng Chin Hin , Chng Wee-Joo 
Published: January 2017

AimsIn recent years, genomic technologies have enabled the identification of mutations in acute myeloid leukaemia (AML).DNMT3Ais a recurrently mutated epigenetic modifier gene in AML. To date, the prognostic significance ofDNMT3Amutations has not been studied in a Southeast Asian AML population. We sought to investigate the clinical implications ofDNMT3Amutations in a Southeast Asian cohort of AML patients.MethodsDNMT3Amutations were identified using a targeted next-generation sequencing panel in 157 AML patients. We studied the molecular and clinical features of patients withDNMT3Amutations and assessed the prognostic impact ofDNMT3Amutations.ResultsDNMT3Amutations were found in 33 of 157 (21.0%) AML patients. 114 patients were included for statistical analysis. Pretreatment data revealed that patients withDNMT3Amutations were older (≥60 years old), had a higher white blood cell count at diagnosis, had more adverse cytogenetic risk profiles and were more often associated withNPM1mutations compared with patients with wild-typeDNMT3A. Survival analysis showed that DNMT3A mutations were associated with poorer clinical outcomes. This was especially when associated with NPM1 and FLT3-ITD mutations (AMLNPM1/FLT3/DNMT3A), which are common. The AMLNPM1/FLT3/DNMT3Asubtype was an independent predictor for poorer overall survival (OS). Other independent risk factors for poorer OS include advanced age at diagnosis and adverse cytogenetic risk stratification.ConclusionsDNMT3Amutations are associated with an unfavourable clinical outcome in our Southeast Asian AML patient cohort. In particular, AMLNPM1/FLT3/DNMT3Apatients had the poorest prognosis.
Cost minimisation analysis of thermometry in two different hospital systems
Authors: Hayes Kenneth , Shepard Amanda , Cesarec August , Likić Robert 
Published: January 2017

ABSTRACTIntroductionTemperature monitoring can be accomplished by various methods, including oral (OT), rectal (RT), axillary (AT), tympanic membrane (TMT) and temporal artery (TAT) thermometry, with varying amounts of cost incurred by healthcare systems.MethodsThe potential thermometry cost savings in two hospital systems—University Hospital Centre Zagreb (UHCZ), which uses TMT (device Covidien Genius 2) and University of Michigan Hospitals (UMH), which relies on OT, RT and AT (device Welch Allyn suretemp plus 692)—were analysed to evaluate institution-wide TAT (device Exergen TAT-5000) implementation. Two scenarios were developed: scenario 1, comparing costs for a period of 1, 3 and 5 years; scenario 2, calculation of the number of measurements per device for TAT to be cost-effective.ResultsAt UHCZ, use of TAT would bring budget savings regardless of the number of devices per bed and the number of years observed. Savings would range from US$0.08 million (one device per bed, impact for 1 year) to US$1.8 million (one device per 10 beds, impact for 5 years). At UMH, use of TAT would lead to budget savings if one device per 10 beds were acquired, but only over a period of 3 or 5 years. Other TAT scenarios were associated with budget costs at UMH even after a period of 5 years.ConclusionsSensitivity analyses showed that the price of current consumables had the highest impact on the model in both hospital settings, with TAT up to 10 times cheaper in that regard over TMT at UHCZ, potentially leading to considerable budget savings within a year of hospital-wide implementation.
Synergy of glucagon-like peptide-2 and epidermal growth factor coadministration on intestinal adaptation in neonatal piglets with short bowel syndrome
Authors: Lim David W. , Levesque Crystal L. , Vine Donna F. , Muto Mitsuru , Koepke Jacob R. , Nation Patrick N. , Wizzard Pamela R. , Li Julang , Bigam David L. , Brubaker Patricia L. , Turner Justine M. , Wales Paul W. 
Published: January 2017

Glucagon-like peptide-2 (GLP-2) and epidermal growth factor (EGF) treatment enhance intestinal adaptation. To determine whether these growth factors exert synergistic effects on intestinal growth and function, GLP-2 and EGF-containing media (EGF-cm) were administered, alone and in combination, in neonatal piglet models of short bowel syndrome (SBS). Neonatal Landrace-Large White piglets were block randomized to 75% midintestinal [jejunoileal (JI) group] or distal intestinal [jejunocolic (JC) group] resection or sham control, with 7-day infusion of saline (control), intravenous human GLP-2 (11 nmol·kg−1·day−1) alone, enteral EGF-cm (80 μg·kg−1·day−1) alone, or GLP-2 and EGF-cm in combination. Adaptation was assessed by intestinal length, histopathology, Üssing chamber analysis, and real-time quantitative PCR of intestinal growth factors. Combined EGF-cm and GLP-2 treatment increased intestinal length in all three surgical models ( P < 0.01). EGF-cm alone selectively increased bowel weight per length and jejunal villus height in the JI group only. The JC group demonstrated increased intestinal weight and villus height ( P < 0.01) when given either GLP-2 alone or in combination with EGF-cm, with no effect of EGF-cm alone. Jejunal permeability of mannitol and polyethylene glycol decreased with combination therapy in both SBS groups ( P < 0.05). No difference was observed in fat absorption or body weight gain. IGF-1 mRNA was differentially expressed in JI vs. JC piglets with treatment. Combined treatment with GLP-2 and EGF-cm induced intestinal lengthening and decreased permeability, in addition to the trophic effects of GLP-2 alone. Our findings demonstrate the benefits of novel combination GLP-2 and EGF treatment for neonatal SBS, especially in the JC model representing most human infants with SBS. NEW & NOTEWORTHY Glucagon-like peptide-2 (GLP-2) and epidermal growth factor (EGF) are intestinotrophic, with demonstrated benefit in both animal models and human studies of short bowel syndrome (SBS). The current research shows that over and above known trophic effects, the combination of GLP-2 and EGF synergistically lengthens the bowel in neonatal piglet models of SBS. Most notable benefit occurred with resection of the terminal ileum, the common clinical anatomy seen in neonatal SBS and associated with least de novo lengthening postsurgery.
Gene networks in skeletal muscle following endurance exercise are coexpressed in blood neutrophils and linked with blood inflammation markers
Authors: Broadbent James , Sampson Dayle , Sabapathy Surendran , Haseler Luke J. , Wagner Karl-Heinz , Bulmer Andrew C. , Peake Jonathan M. , Neubauer Oliver 
Published: January 2017

It remains incompletely understood whether there is an association between the transcriptome profiles of skeletal muscle and blood leukocytes in response to exercise or other physiological stressors. We have previously analyzed the changes in the muscle and blood neutrophil transcriptome in eight trained men before and 3, 48, and 96 h after 2 h cycling and running. Because we collected muscle and blood in the same individuals and under the same conditions, we were able to directly compare gene expression between the muscle and blood neutrophils. Applying weighted gene coexpression network analysis (WGCNA) as an advanced network-driven method to these original data sets enabled us to compare the muscle and neutrophil transcriptomes in a rigorous and systematic manner. Two gene networks were identified that were preserved between skeletal muscle and blood neutrophils, functionally related to mitochondria and posttranslational processes. Strong preservation measures ( Zsummary > 10) for both muscle-neutrophil gene networks were evident within the postexercise recovery period. Muscle and neutrophil gene coexpression was strongly correlated in the mitochondria-related network ( r = 0.97; P = 3.17E-2). We also identified multiple correlations between muscular gene subnetworks and exercise-induced changes in blood leukocyte counts, inflammation, and muscle damage markers. These data reveal previously unidentified gene coexpression between skeletal muscle and blood neutrophils following exercise, showing the value of WGCNA to understand exercise physiology. Furthermore, these findings provide preliminary evidence in support of the notion that blood neutrophil gene networks may potentially help us to track physiological and pathophysiological changes in the muscle. NEW & NOTEWORTHY By using weighted gene coexpression network analysis, an advanced bioinformatics method, we have identified previously unknown, functional gene networks that are preserved between skeletal muscle and blood neutrophils during recovery from exercise. These novel preliminary data suggest that muscular gene networks are coexpressed in blood leukocytes following physiological stress. This is a step forward toward the development of blood neutrophil gene subnetworks as part of blood biomarker panels to assess muscle health and disease.
How does passive lengthening change the architecture of the human medial gastrocnemius muscle?
Authors: Bolsterlee Bart , D’Souza Arkiev , Gandevia Simon C. , Herbert Robert D. 
Published: January 2017

There are few comprehensive investigations of the changes in muscle architecture that accompany muscle contraction or change in muscle length in vivo. For this study, we measured changes in the three-dimensional architecture of the human medial gastrocnemius at the whole muscle level, the fascicle level and the fiber level using anatomical MRI and diffusion tensor imaging (DTI). Data were obtained from eight subjects under relaxed conditions at three muscle lengths. At the whole muscle level, a 5.1% increase in muscle belly length resulted in a reduction in both muscle width (mean change −2.5%) and depth (−4.8%). At the fascicle level, muscle architecture measurements obtained at 3,000 locations per muscle showed that for every millimeter increase in muscle-tendon length above the slack length, average fascicle length increased by 0.46 mm, pennation angle decreased by 0.27° (0.17° in the superficial part and 0.37° in the deep part), and fascicle curvature decreased by 0.18 m−1. There was no evidence of systematic variation in architecture along the muscle’s long axis at any muscle length. At the fiber level, analysis of the diffusion signal showed that passive lengthening of the muscle increased diffusion along fibers and decreased diffusion across fibers. Using these measurements across scales, we show that the complex shape changes that muscle fibers, whole muscles, and aponeuroses of the medial gastrocnemius undergo in vivo cannot be captured by simple geometrical models. This justifies the need for more complex models that link microstructural changes in muscle fibers to macroscopic changes in architecture. NEW & NOTEWORTHY Novel MRI and DTI techniques revealed changes in three-dimensional architecture of the human medial gastrocnemius during passive lengthening. Whole muscle belly width and depth decreased when the muscle lengthened. Fascicle length, pennation, and curvature changed uniformly or near uniformly along the muscle during passive lengthening. Diffusion of water molecules in muscle changes in the same direction as fascicle strains.
Venetoclax in Patients with Previously Treated Chronic Lymphocytic Leukemia
Authors: Roberts Andrew W. , Stilgenbauer Stephan , Seymour John F. , Huang David C.S. 
Published: January 2017

Abstract
Venetoclax is the first BCL2 inhibitor to enter routine clinical practice. It is an orally bioavailable small molecule that binds BCL2 very specifically. Acting as a pharmacologic mimic of the proteins that initiate apoptosis (a so-called BH3 mimetic), venetoclax rapidly induces apoptosis in chronic lymphocytic leukemia (CLL) cells, which express high levels of BCL2 and rely on it to maintain their survival. As a single agent, daily venetoclax treatment induced durable responses in 79% of patients with relapsed or refractory CLL or small lymphocytic lymphoma in a phase I study, including complete remissions in 20% of patients. Its use was approved by the FDA in April 2016 for patients with previously treated del(17p) CLL on the basis of a single-arm phase II trial demonstrating a 79% response rate and an estimated 1-year progression-free survival of 72% with 400 mg/day continuous therapy. This review focuses on venetoclax, its mechanism of action, pharmacology, and clinical trial data and seeks to place it in the context of rapid advances in therapy for patients with relapsed CLL, especially those with del(17p) CLL. Clin Cancer Res; 23(16); 4527–33. ©2017 AACR.
Effect of Metformin on Metabolites and Relation With Myocardial Infarct Size and Left Ventricular Ejection Fraction After Myocardial Infarction
Authors: Eppinga Ruben N. , Kofink Daniel , Dullaart Robin P.F. , Dalmeijer Geertje W. , Lipsic Erik , van Veldhuisen Dirk J. , van der Horst Iwan C.C. , Asselbergs Folkert W. , van der Harst Pim 
Published: January 2017

Background—
Left ventricular ejection fraction (LVEF) and infarct size (ISZ) are key predictors of long-term survival after myocardial infarction (MI). However, little is known about the biochemical pathways driving LV dysfunction after MI. To identify novel biomarkers predicting post-MI LVEF and ISZ, we performed metabolic profiling in the GIPS-III randomized clinical trial (Glycometabolic Intervention as Adjunct to Primary Percutaneous Intervention in ST Elevation Myocardial Infarction). We also investigated the metabolic footprint of metformin, a drug associated with improved post-MI LV function in experimental studies.

Methods and Results—

Participants were patients with ST-segment–elevated MI who were randomly assigned to receive metformin or placebo for 4 months. Blood samples were obtained on admission, 24 hours post-MI, and 4 months post-MI. A total of 233 metabolite measures were quantified using nuclear magnetic resonance spectrometry. LVEF and ISZ were assessed 4 months post-MI. Twenty-four hours post-MI measurements of high-density lipoprotein (HDL) triglycerides (HDL-TG) predicted LVEF (β=1.90 [95% confidence interval (CI), 0.82 to 2.98];
P
=6.4×10
−4
) and ISZ (β=−0.41 [95% CI, −0.60 to −0.21];
P
=3.2×10
−5
). In addition, 24 hours post-MI measurements of medium HDL-TG (β=−0.40 [95% CI, −0.60 to −0.20];
P
=6.4×2×10
−5
), small HDL-TG (β=−0.34 [95% CI, −0.53 to −0.14];
P
=7.3×10
−4
), and the triglyceride content of very large HDL (β=−0.38 [95% CI, −0.58 to −0.18];
P
=2.7×10
−4
) were associated with ISZ. After the 4-month treatment, the phospholipid content of very large HDL was lower in metformin than in placebo-treated patients (28.89% versus 38.79%;
P
=7.5×10
−5
); alanine levels were higher in the metformin group (0.46 versus 0.44 mmol/L;
P
=2.4×10
−4
).

Conclusions—
HDL triglyceride concentrations predict post-MI LVEF and ISZ. Metformin increases alanine levels and reduces the phospholipid content in very large HDL particles.

Clinical Trial Registration—

URL:
https://clinicaltrials.gov/ct2/show/NCT01217307
. Unique Identifier: NCT01217307.
Reprocessed, Bias-Corrected CMORPH Global High-Resolution Precipitation Estimates from 1998
Authors: Xie Pingping , Joyce Robert , Wu Shaorong , Yoo Soo-Hyun , Yarosh Yelena , Sun Fengying , Lin Roger 
Published: January 2017

Abstract
The Climate Prediction Center (CPC) morphing technique (CMORPH) satellite precipitation estimates are reprocessed and bias corrected on an 8 km × 8 km grid over the globe (60°S–60°N) and in a 30-min temporal resolution for an 18-yr period from January 1998 to the present to form a climate data record (CDR) of high-resolution global precipitation analysis. First, the purely satellite-based CMORPH precipitation estimates (raw CMORPH) are reprocessed. The integration algorithm is fixed and the input level 2 passive microwave (PMW) retrievals of instantaneous precipitation rates are from identical versions throughout the entire data period. Bias correction is then performed for the raw CMORPH through probability density function (PDF) matching against the CPC daily gauge analysis over land and through adjustment against the Global Precipitation Climatology Project (GPCP) pentad merged analysis of precipitation over ocean. The reprocessed, bias-corrected CMORPH exhibits improved performance in representing the magnitude, spatial distribution patterns, and temporal variations of precipitation over the global domain from 60°S to 60°N. Bias in the CMORPH satellite precipitation estimates is almost completely removed over land during warm seasons (May–September), while during cold seasons (October–April) CMORPH tends to underestimate the precipitation due to the less-than-desirable performance of the current-generation PMW retrievals in detecting and quantifying snowfall and cold season rainfall. An intercomparison study indicated that the reprocessed, bias-corrected CMORPH exhibits consistently superior performance than the widely used TRMM 3B42 (TMPA) in representing both daily and 3-hourly precipitation over the contiguous United States and other global regions.
On the Nature of the Mesoscale Variability in Denmark Strait
Authors: Appen Wilken-Jon von , Mastropole Dana , Pickart Robert S. , Valdimarsson HéÐinn , Jónsson Steingrímur , Girton James B. 
Published: January 2017

AbstractTime series data from a mooring in the center of Denmark Strait and a collection of shipboard hydrographic sections occupied across the sill are used to elucidate the mesoscale variability of the dense overflow water in the strait. Two dominant, reoccurring features are identified that are referred to as a bolus and a pulse. A bolus is a large, weakly stratified lens of overflow water associated with cyclonic rotation and a modest increase in along-stream speed of 0.1 m s−1. When a bolus passes through the strait the interface height of the overflow layer increases by 60 m, and the bottom temperature decreases by 0.4°C. By contrast, a pulse is characterized by anticyclonic rotation, a strong increase in along-stream speed of >0.25 m s−1, a decrease in interface height of 90 m, and no significant bottom temperature signal. It is estimated that, on average, boluses (pulses) pass through the strait every 3.4 (5.4) days with no seasonal signal to their frequency. Both features have the strongest along-stream signal in the overflow layer, while the strongest cross-stream velocities occur above the Denmark Strait overflow water (DSOW). In this sense neither feature can be characterized as a simple propagating eddy. Their dynamics appear to be similar to that ascribed to the mesoscale variability observed downstream in the deep western boundary current. Strong correlation of bottom temperatures between the mooring in Denmark Strait and a downstream array, together with a match in the frequency of occurrence of features at both locations, suggests a causal relationship between the mesoscale variability at the sill and that farther downstream.
Effects of Post-Detection Response Complexity on Subsequent Monitoring Behavior
Authors: Monty Richard A. 
Published: January 2017

This paper examines the effect of the complexity of the post-detection response demands on subsequent monitoring performance at two rates of target presentation. Three conditions requiring differentially complex post-detection responses were compared with a baseline condition in which no post-detection demands were made (save the simple detection-indicating response). The results indicated that two of the three complex conditions were accompanied by significantly less decrement in performance than that found in the baseline condition. The third was not significantly different from the baseline condition. “Arousal theory” could account for the results on the basis that there exists an optimal level of cortical arousal stemming from sensory variation provided by a task. If this hypothetical level is not reached or exceeded, a decrement in performance is to be expected. No clear answer was provided regarding an interaction between effects attributable to signal rate with effects attributable to response complexity.
The Effect of Rate and Direction of Display Movement upon Visual Search
Authors: Williams Leon G. , Borow Marion S. 
Published: January 2017

A study was undertaken to determine the effects of rate and direction of display movement upon the time to find alphabetical targets. Subjects searched for targets which always remained present in a display which moved but did not change. Display speeds somewhat faster than 8 degrees/sec angular velocity (2 in./sec viewed at 15 in.) were associated with decrements in performance. At higher rates of movement, search times for horizontally-moving displays were lower than those for vertically-moving displays. It is recommended that high-speed displays move horizontally when compatible with other system needs.