bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Ketorolac After Outpatient Urogynecologic Surgery: A Randomized Control Trial
Authors: Trowbridge Elisa R. , Kim Dahea , Caldwell Lauren , Franko Denise , Jackson Jessica Nicole , Shilling Ashley , Hullfish Kathie L. 
Published: September 2017

Objective
Patient surveys highlight a prevalence of moderate to severe pain in the postanesthesia care unit. Multimodal analgesia has been promoted to improve this with fewer opioid-induced adverse effects. The aim of this study was to evaluate the opioid sparing and analgesic effect of postoperative intravenous (IV) ketorolac after outpatient transvaginal surgery.

Methods
Forty patients were enrolled in this institutional review board–approved, randomized, double-blind, placebo-controlled study, to receive either 30 mg of IV ketorolac or IV saline placebo postoperatively. Pain was assessed by visual analog scale at timed intervals. Narcotic pain medication was provided upon request. Narcotic use was reassessed by telephone 5 to 7 days postoperatively. Categorical characteristics were compared by χ2. Continuous variables were evaluated by Mann-Whitney U test.

Results
Twenty patients were randomized to each group. Groups were similar in age, health, and operative factors. There was no significant difference in mean pain scores at any interval. The ketorolac group had a total morphine equivalent consumption median of 7.5 mg versus 4.0 mg for placebo, which was not significant (P = 0.17). Total use of narcotic pills postoperatively was equivalent (median, 5). There was no difference in postoperative nausea. One Dindo grade II complication was reported in the ketorolac group of a postoperative pelvic hematoma requiring transfusion.

Discussion
Intravenous ketorolac administered after outpatient transvaginal surgery did not result in a reduction of pain scores or total morphine consumption. There was one Dindo grade II complication in the ketorolac group. Larger randomized control trials are needed to validate these findings.
Length of Catheter Use After Hysterectomy as a Risk Factor for Urinary Tract Infection
Authors: Karp Natalie E. , Kobernik Emily K. , Kamdar Neil S. , Fore Amanda M. , Morgan Daniel M. 
Published: September 2017

Objectives
The aims of this study were to determine the effect of length of postoperative catheterization on risk of urinary tract infection (UTI) and to identify risk factors for postoperative UTI.

Methods
This was a retrospective case-control study. Demographic and perioperative data, including duration of indwelling catheter use and postoperative occurrence of UTI within 30 days of surgery, were analyzed for hysterectomies using the Michigan Surgical Quality Collaborative database. Catheter exposure was categorized as low—no catheter placed/catheter removed the day of surgery, intermediate—catheter removed postoperative day 1, high—catheter removal on postoperative day 2 or later, or highest—patient discharged home with catheter. A multivariable logistic regression model was developed to identify factors associated with UTI. An interaction term was included in the final model.

Results
Overall, UTI prevalence was 2.3% and increased with duration of catheter exposure (low: 1.3% vs intermediate: 2.1% vs high: 4.1% vs highest: 6.5%, P < 0.0001). High (odds ratio [OR] = 2.54 [1.51–4.27]) and highest (OR = 3.39 [1.86–6.17]) catheter exposure, operative time (OR = 1.15 [1.03–1.29]), and dependent functional status (OR = 4.62 [1.90–11.20]) were independently associated with UTI. Women who had a vaginal hysterectomy with sling/pelvic organ prolapse repair were more likely to have a UTI than those who had a vaginal hysterectomy alone (OR = 2.58 [1.10–6.07]) and more likely to have a UTI than women having an abdominal or laparoscopic hysterectomy with a sling/pelvic organ prolapse repair (OR = 2.13 [1.12–4.04]).

Conclusions
Length of catheterization and operative time are modifiable risk factors for UTI after hysterectomy. An interaction between vaginal hysterectomy and concomitant pelvic reconstruction increases the odds of UTI.
Discordant association of theCREBRFrs373863828 minor allele with increased body mass index and protection from type 2 diabetes in Māori and Pacific (Polynesian) people living in Aotearoa New Zealand
Authors: Krishnan Mohanraj , Major Tanya J , Topless Ruth K , Dewes Ofa , Yu Lennex , Thompson John MD , McCowan Lesley , de Zoysa Janak , Stamp Lisa K , Dalbeth Nicola , Hindmarsh Jennie Harré , Rapana Nuku , Deka Ranjan , Eng Winston W H , Weeks Daniel E , Minster Ryan L , McGarvey Stephen T , Viali Satupa’itea , Naseri Take , Reupena Muagututi’a Sefuiva , Wilcox Phillip , Grattan David , Shepherd Peter R , Shelling Andrew N , Murphy Rinki , Merriman Tony R 
Published: September 2017

AbstractAim/HypothesesThe minor allele ofCREBRFrs373863828 associates with increased body mass index (BMI) and reduced risk of type 2 diabetes (T2D) in the Samoan population of Samoa and American Samoa. Our aim was to testrs373863828for association with BMI and odds of T2D, gout and chronic kidney disease (CKD) in Māori and Pacific (Polynesian) people living in Aotearoa New Zealand in 2,286 adults.MethodsAssociation analyses were performed by linear and logistic regression with BMI, log-transformed BMI, waist circumference, T2D, gout and CKD. Analyses were adjusted for age, sex, the first four genome-wide principal components, and (when appropriate) BMI, waist circumference and T2D.ResultsFor the minor allele of rs373863828 the effect size for log-transformed BMI was 0.038 (95% CI [0.022-0.055],P=4.8x10−6) and for T2D was OR=0.59 (95% CI [0.47-0.73],P=1.9x10−6). There was no evidence for association of genotype with variance in BMI (P=0.13). Nor was there evidence for association with serum urate (β=0.012 mmol/L,Pc=0.10), gout (OR=1.00,P=0.98) or CKD (OR=0.91,P=0.59).Conclusions/interpretationOur results replicated, with very similar effect sizes, association of the minor allele of rs373863828 with higher BMI but lower odds of T2D among New Zealand Polynesian adults, as in Samoan adults living in Samoa and American Samoa.
Structure of BrlR reveals a potential pyocyanin binding site
Authors: Raju Harikiran , Sundararajan Rukmini , Sharma Rohan 
Published: September 2017

AbstractThe transcriptional regulator BrlR from Pseudomonas aeruginosa is a member of the MerR family of multidrug transport activators. Studies have shown BrlR plays an important role in high level drug tolerance of P. aeruginosa in biofilm. Its drug tolerance ability can be enhanced by 3′,5′-cyclic diguanylic acid (c-di-GMP). Here, we show the apo structure of BrlR and the direct binding between GyrI-like domain of BrlR and P. aeruginosa toxin pyocyanin. Furthermore, pyocyanin can enhance the binding between BrlR and DNA in vitro. These findings suggest BrlR can serve as the binding partner for both c-di-GMP and pyocyanin.
Endogenous α-SYN protein analysis from human brain tissues using single-molecule pull-down assay
Authors: Je Goun , Croop Benjamin , Basu Sambuddha , Tang Jialei , Han Kyu Young , Kim Yoon-Seong 
Published: September 2017

AbstractAlpha-synuclein (α-SYN) is a central molecule in Parkinson’s disease pathogenesis. Despite several studies, the molecular nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of bio-specimens. Here, we introduce α-SYN single-molecule pull-down (α-SYN SiMPull) assay combined with in vivo protein crosslinking to count individual α-SYN protein and assess its native oligomerization states from biological samples including human postmortem brains. This powerful single-molecule assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer’s disease and Parkinson’s disease.
Comparison of GWAS models to identify non-additive genetic control of flowering time in sunflower hybrids
Authors: Bonnafous Fanny , Fievet Ghislain , Blanchet Nicolas , Boniface Marie-Claude , Carrère Sébastien , Gouzy Jérôme , Legrand Ludovic , Marage Gwenola , Bret-Mestries Emmanuelle , Munos Stéphane , Pouilly Nicolas , Vincourt Patrick , Langlade Nicolas , Mangin Brigitte 
Published: September 2017

AbstractGenome-wide association studies are a powerful and widely used tool to decipher the genetic control of complex traits. One of the main challenges for hybrid crops, such as maize or sunflower, is to model the hybrid vigor in the linear mixed models, considering the relatedness between individuals. Here, we compared two additive and three non-additive association models for their ability to identify genomic regions associated with flowering time in sunflower hybrids. A panel of 452 sunflower hybrids, corresponding to incomplete crossing between 36 male lines and 36 female lines, was phenotyped in five environments and genotyped for 2,204,423 SNPs. Intra-locus effects were estimated in multi-locus models to detect genomic regions associated with flowering time using the different models. Thirteen quantitative trait loci were identified in total, two with both model categories and one with only non-additive models. A quantitative trait loci on LG09, detected by both the additive and non-additive models, is located near a GAI homolog and is presented in detail. Overall, this study shows the added value of non-additive modeling of allelic effects for identifying genomic regions that control traits of interest and that could participate in the heterosis observed in hybrids.
Genome-wide analysis of genetic risk factors for rheumatic heart disease in Aboriginal Australians provides support for pathogenic molecular mimicry
Authors: Gray Lesley-Ann , D’Antoine Heather A , Tong Steven Y. C. , McKinnon Melita , Bessarab Dawn , Brown Ngiare , Reményi Bo , Steer Andrew , Syn Genevieve , Blackwell Jenefer M , Inouye Michael , Carapetis Jonathan R 
Published: September 2017

AbstractBackgroundRheumatic heart disease (RHD) following Group A Streptococcus (GAS) infections is heritable and prevalent in Indigenous populations. Molecular mimicry between human and GAS proteins triggers pro-inflammatory cardiac valve-reactive T-cells.MethodsGenome-wide genetic analysis was undertaken in 1263 Aboriginal Australians (398 RHD cases; 865 controls). Single nucleotide polymorphisms (SNPs) were genotyped using Illumina HumanCoreExome BeadChips. Direct typing and imputation was used to fine-map the human leukocyte antigen (HLA) region. Epitope binding affinities were mapped for human cross-reactive GAS proteins, including M5 and M6.ResultsThe strongest genetic association was intronic to HLA-DQA1 (rs9272622; P=1.86x10−7). Conditional analyses showed rs9272622 and/or DQA1*AA16 account for the HLA signal. HLA-DQA1*0101_DQB1*0503 (OR 1.44, 95%CI 1.09-1.90, P=9.56x10−3) and HLA-DQA1*0103_DQB1*0601 (OR 1.27, 95%CI 1.07-1.52, P=7.15x10−3) were risk haplotypes; HLA_DQA1*0301-DQB1*0402 (OR 0.30, 95%CI 0.14-0.65, P=2.36x10−3) was protective. Human myosin cross-reactive N-terminal and B repeat epitopes of GAS M5/M6 bind with higher affinity to DQA1/DQB1 alpha/beta dimers for the two risk haplotypes than the protective haplotype.ConclusionsVariation at HLA_DQA1-DQB1 is the major genetic risk factor for RHD in Aboriginal Australians studied here. Cross-reactive epitopes bind with higher affinity to alpha/beta dimers formed by risk haplotypes, supporting molecular mimicry as the key mechanism of RHD pathogenesis.
Evidence for a long-term protection of wheel-running exercise against cocaine psychomotor sensitization in adolescent but not in young adult mice
Authors: Lespine Louis-Ferdinand , Tirelli Ezio 
Published: September 2017

AbstractRodents housed with a running wheel can exhibit attenuated cocaine seeking and cocaine-induced psychomotor activation. However, the longevity of the exercise anti-drug protection and the influence of the developmental stage during which exercise is displayed received little attention. Here, females and males C57BL/6J mice, aged 28 (adolescents) or 77 (young adults) days were housed with (n=56) or without (n=28) a running wheel. After 3 weeks in these conditions, half of the exercised mice were deprived of their wheel (n=28) whereas the other half and the sedentary mice (no wheel) were kept in their respective environments throughout experimentation. After 3 additional weeks, mice were tested for initiation of psychomotor sensitization to 9 once-daily intraperitoneal injections of 8 mg/kg cocaine (following 2 drug-free test sessions). The expression of sensitization was assessed on a single test session 30 days after the last sensitizing cocaine injection. Continuously exercised mice (wheel throughout experimentation) were less responsive to the initiation and the expression of cocaine effects, regardless of the gender and the developmental period during which exercise was introduced. Wheel-running during adolescence attenuated in later life the initiation and the expression of sensitization in females and only its expression in males. In adult females and males, previously-exercised and sedentary mice exhibited indiscernible levels of initiation and expression of sensitization. Thus, the likelihood of the long-term protection of exercise against cocaine vulnerability may depend not only on the gender but also and especially on the period of life in which exercise took place.
clusterSeq: methods for identifying co-expression in high-throughput sequencing data
Authors: Hardcastle Thomas J. , Papatheodorou Irene 
Published: September 2017

ABSTRACTSummary:Identifying gene co-expression is a significant step in understanding functional relationships between genes. Existing methods primarily depend on analyses of correlation between pairs of genes; however, this neglects structural elements between experimental conditions. We present a novel approach to identifying clusters of co-expressed genes that incorporates these structures.Availability:The methods are released on Bioconductor as the clusterSeq package (https://bioconductor.org/packages/release/bioc/html/clusterSeq.html).Contact:tjh48@cam.ac.uk
AFFIRM employees to reduce cynicism in the workplace
Authors: Schraeder Mike , Schraeder Hannah 
Published: September 2017

Purpose
The purpose of this paper is to describe the potential value of affirmation as a way that leaders might be able to reduce employee cynicism in organizations.

Design/methodology/approach
A brief overview of cynicism and possible value of affirmation as a method to reduce cynicism is followed by suggestions for ways that leaders may be able to reduce cynicism through affirmation. Six strategies for reducing cynicism were summarized. These strategies were organized using AFFIRM as an acrostic.

Findings
Leaders may be able to reduce employee cynicism through actions intended to affirm employees. Specifically, it is suggested that leaders can affirm employees through acknowledgement and appreciation, feedback, focus, involvement, respect, and maintaining employee well-being.

Originality/value
This paper framed the strategies for reducing cynicism using AFFIRM as an acrostic for the six strategies. Organizing the recommended strategies of acknowledgement/appreciation, feedback, focus, involvement, respect, and maintaining employee well-being using this methodology may be a beneficial tool for leaders looking for ways to reduce employee cynicism in organizations.
A study for steady nanofluid flow between parallel plates
Authors: Mohyud-din Syed Tauseef , Asad Iqbal Muhammad , Shakeel Muhammad 
Published: September 2017

Purpose
In this paper, the authors study the behavior of heat and mass transfer between parallel plates of a steady nanofluid flow in the presence of a uniform magnetic field. In the model of nanofluids, the essential effect of thermophoresis and Brownian motion has been encompassed.

Design/methodology/approach
The variation of parameters method has been exploited to solve the differential equations of nanofluid model. The legitimacy of the variation of parameters method has been corroborated by a comparison of foregoing works by many authors on viscous fluid.

Findings
An analysis of the model is performed for different parameters, namely, viscosity parameter, Brownian parameter, thermophoretic parameter and magnetic parameter.

Originality/value
The variation of parameters method proves to be very effective in solving nonlinear system of ordinary differential equations which frequently arise in fluid mechanics.
Reflexive convention: civil partnership, marriage and family
Authors: Heaphy Brian 
Published: September 2017

AbstractDrawing on an analysis of qualitative interview data from a study of formalized same‐sex relationships (civil partnerships) this paper examines the enduring significance of marriage and family as social institutions. In doing so, it intervenes in current debates in the sociology of family and personal life about how such institutions are undermined by reflexivity or bolstered by convention. Against the backdrop of dominating sociological frames for understanding the links between the changing nature of marriage and family and same‐sex relationship recognition, the paper analyses the diverse and overlapping ways (including the simple, relational, strategic, ambivalent and critical ways) in which same‐sex partners reflexively constructed and engaged with marriage and family conventions. My analysis suggests that instead of viewing reflexivity and convention as mutually undermining, as some sociologists of family and personal life do, it is insightful to explore how diverse forms of reflexivity and convention interact in everyday life to reconfigure the social institutions of marriage and family, but do not undermine them as such. I argue the case for recognizing the ways in which ‘reflexive convention’, or reflexive investment in convention, contributes to the continuing significance of marriage and family as social institutions.
Nursing Care Disparities in Neonatal Intensive Care Units
Authors: Lake Eileen T. , Staiger Douglas , Edwards Erika Miles , Smith Jessica G. , Rogowski Jeannette A. 
Published: September 2017

ObjectivesTo describe the variation across neonatal intensive care units (NICUs) in missed nursing care in disproportionately black and non‐black‐serving hospitals. To analyze the nursing factors associated with missing nursing care.Data Sources/Study SettingSurvey of random samples of licensed nurses in four large U.S. states.Study DesignThis was a retrospective, secondary analysis of 1,037 staff nurses in 134 NICUs classified into three groups based on their percent of infants of black race. Measures included the average patient load, individual nurses’ patient loads, professional nursing characteristics, nurse work environment, and nursing care missed on the last shift.Data CollectionSurvey data from a Multi‐State Nursing Care and Patient Safety Study were analyzed (39 percent response rate).Principal FindingsThe patient‐to‐nurse ratio was significantly higher in high‐black hospitals. Nurses in high‐black NICUs missed nearly 50 percent more nursing care than in low‐black NICUs. Lower nurse staffing (an additional patient per nurse) significantly increased the odds of missed care, while better practice environments decreased the odds.ConclusionsNurses in high‐black NICUs face inadequate staffing. They are more likely to miss required nursing care. Improving staffing and workloads may improve the quality of care for the infants born in high‐black hospitals.
Assessment of futility in out‐of‐hospital cardiac arrest
Authors: Setälä P. , Hoppu S. , Virkkunen I. , Yli‐Hankala A. , Kämäräinen A. 
Published: September 2017

BackgroundOur aim was to evaluate the impact of futile resuscitation attempts to the outcome calculations of attempted resuscitation in out‐of‐hospital cardiac arrest (OHCA). Defined as partial resuscitations, we focused on a subgroup of patients in whom cardiopulmonary resuscitation (CPR) was initiated, but further efforts were soon abandoned due to evidence of futility.MethodsWe conducted this study using the Utstein template during a 12‐month study period. We compared the event characteristics between full and partial resuscitation attempts and determined the incidence, survival and neurological outcome.ResultsEmergency Medical Services (EMS) attended a total of 314 OHCA cases. In 34 cases, resuscitation was not attempted due to futility. Seventy‐four cases were partial resuscitation attempts where resuscitation was soon discontinued due to dismal prognostic factors. Partial attempts were associated with an unwitnessed OHCA, prolonged downtime, end‐stage malignant disease, multiple trauma, asystole or pulseless electrical activity as the initial rhythm, and a first responding unit being the first unit on the scene (P < 0.05, respectively). The calculation of survival to hospital discharge rate was 14% and increased 5% when partial resuscitation attempts were excluded from the analysis. Seventy‐four percentage had a Cerebral Performance Category 1–2 at hospital discharge. Shockable initial rhythm, public location and bystander CPR had a positive impact on survival.ConclusionsResuscitative efforts were considered futile in 11% of cases and resuscitation was discontinued due to evidence of futility in additional 24% cases based on additional information. Terminating resuscitation should be identified as a separate subgroup of OHCA cases to better reflect the outcome.
Understanding nomadic collaborative learning groups
Authors: Ryberg Thomas , Davidsen Jacob , Hodgson Vivien 
Published: September 2017

AbstractThe paper builds on the work of Rossitto et al. on collaborative nomadic work to develop three categories of practice of nomadic collaborative learning groups. Our study is based on interviews, workshops and observations of two undergraduate student's group practices engaged in self‐organised, long‐term collaborations within the frame of Problem and Project Based Learning. By analysing the patterns of nomadic collaborative learning we identify and discuss how the two groups of students incorporate mobile and digital technologies as well as physical and/or non‐digital technologies into their group work. Specifically, we identify the following categories of nomadic collaborative learning practices: “orchestration of work phases, spaces and activities,” “the orchestration of multiple technologies” and “orchestration of togetherness.” We found that for both groups of students there was a fluidity, situatedness and improvisational aspect to how they negotiate the orchestration of their work. Their ways of utilising space, places, technologies and activities over time was a complex interweaving of the digital and physical. We conclude by suggesting that the three categories of practice identified are important for deepening our understanding of nomadic collaborative learning groups.