bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracorporeal membrane oxygenation (ECMO) for critically ill adults in the emergency department: history, current applications, and future directions
Authors: Mosier Jarrod M. , Kelsey Melissa , Raz Yuval , Gunnerson Kyle J. , Meyer Robyn , Hypes Cameron D. , Malo Josh , Whitmore Sage P. , Spaite Daniel W. 
Published: December 2015

AbstractExtracorporeal membrane oxygenation (ECMO) is a mode of extracorporeal life support that augments oxygenation, ventilation and/or cardiac output via cannulae connected to a circuit that pumps blood through an oxygenator and back into the patient. ECMO has been used for decades to support cardiopulmonary disease refractory to conventional therapy. While not robust, there are promising data for the use of ECMO in acute hypoxemic respiratory failure, cardiac arrest, and cardiogenic shock and the potential indications for ECMO continue to increase. This review discusses the existing literature on the potential use of ECMO in critically ill patients within the emergency department.
Genetic modification of human mesenchymal stem cells helps to reduce adiposity and improve glucose tolerance in an obese diabetic mouse model
Authors: Sen Sabyasachi , Domingues Cleyton C. , Rouphael Carol , Chou Cyril , Kim Chul , Yadava Nagendra 
Published: December 2015

Abstract
Introduction
Human mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into fat, muscle, bone and cartilage cells. Exposure of subcutaneous abdominal adipose tissue derived AD-MSCs to high glucose (HG) leads to superoxide accumulation and up-regulation of inflammatory molecules. Our aim was to inquire how HG exposure affects MSCs differentiation and whether the mechanism is reversible.

Methods
We exposed human adipose tissue derived MSCs to HG (25 mM) and compared it to normal glucose (NG, 5.5 mM) exposed cells at 7, 10 and 14 days. We examined mitochondrial superoxide accumulation (Mitosox-Red), cellular oxygen consumption rate (OCR, Seahorse) and gene expression.

Results
HG increased reactive superoxide (ROS) accumulation noted by day 7 both in cytosol and mitochondria. The OCR between the NG and HG exposed groups however did not change until 10 days at which point OCR of HG exposed cells were reduced significantly. We noted that HG exposure upregulated mRNA expression of adipogenic (PPARG, FABP-4, CREBP alpha and beta), inflammatory (IL-6 and TNF alpha) and antioxidant (SOD2 and Catalase) genes. Next, we used AdSOD2 to upregulate SOD2 prior to HG exposure and thereby noted reduction in superoxide generation. SOD2 upregulation helped reduce mRNA over-expression of PPARG, FABP-4, IL-6 and TNFα. In a series of separate experiments, we delivered the eGFP and SOD2 upregulated MSCs (5 days post ex-vivo transduction) and saline intra-peritoneally (IP) to obese diabetic (db/db) mice. We confirmed homing-in of eGFP labeled MSCs, delivered IP, to different inflamed fat pockets, particularly omental fat. Mice receiving SOD2-MSCs showed progressive reduction in body weight and improved glucose tolerance (GTT) at 4 weeks, post MSCs transplantation compared to the GFP-MSC group (control).

Conclusions
High glucose evokes superoxide generation, OCR reduction and adipogenic differentiation. Mitochondrial superoxide dismutase upregulation quenches excess superoxide and reduces adipocyte inflammation. Delivery of superoxide dismutase (SOD2) using MSCs as a gene delivery vehicle reduces inflammation and improves glucose tolerance in vivo. Suppression of superoxide production and adipocyte inflammation using mitochondrial superoxide dismutase may be a novel and safe therapeutic tool to combat hyperglycemia mediated effects.
Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice
Authors: Rodriguez Jonathan , Boucher Fabien , Lequeux Charlotte , Josset-Lamaugarny Audrey , Rouyer Ondine , Ardisson Orianne , Rutschi Héléna , Sigaudo-Roussel Dominique , Damour Odile , Mojallal Ali 
Published: December 2015

AbstractBackgroundThe use of stem cells from adipose tissue or adipose-derived stem cells (ASCs) in regenerative medicine could be an interesting alternative to bone marrow stem cells because they are easily accessible and available in large quantities. The aim of this study was to evaluate the potential effect of ASCs on the healing of 12 mm diameter-excisional wounds (around 110 mm2) in nude mice.MethodsThirty nude mice underwent surgery to create one 12-mm excisional wound per mouse (spontaneous healing, n = 6; Cytocare® 532, n = 12; ASCs, n = 12). The Galiano wound model was chosen to avoid shrinkage and thus slow the spontaneous healing (SH) of mouse skin, making it closer to the physiology of human skin healing. Transparent dressings were used to enable daily healing time measurements to be taken. Immunohistochemistry, histological and blood perfusion analysis were carried out on the healed skin.ResultsThe in vivo results showed the effectiveness of using ASCs on reducing the time needed for complete healing to 21.2 days for SH, 17.4 days for vehicle alone (Cytocare® 532) and 14.6 days with the addition of ASCs (p < 0.001). Moreover, cutaneous perfusion of the healed wound was significantly improved in ASC-treated mice compared to SH group, as shown by laser Doppler flowmetry and the quantitation of blood vessels using immunohistochemistry of αsmooth muscle actin.ConclusionsThe tolerance and efficacy of cryopreserved ASCs to accelerate the complete closure of the wound by increasing the maturation of the skin and its blood perfusion,shows their therapeutic benefit in the wound healing context.
Genome sequence of the clover symbiont Rhizobium leguminosarum bv. trifolii strain CC275e
Authors: Delestre Clément , Laugraud Aurélie , Ridgway Hayley , Ronson Clive , O’Callaghan Maureen , Barrett Brent , Ballard Ross , Griffiths Andrew , Young Sandra , Blond Celine , Gerard Emily , Wakelin Steve 
Published: December 2015

AbstractRhizobium leguminosarum bv. trifolii strain CC275e is a highly effective, N2-fixing microsymbiont of white clover (Trifolium repens L.). The bacterium has been widely used in both Australia and New Zealand as a clover seed inoculant and, as such, has delivered the equivalent of millions of dollars of nitrogen into these pastoral systems. R. leguminosarum strain CC275e is a rod-shaped, motile, Gram-negative, non-spore forming bacterium. The genome was sequenced on an Illumina MiSeq instrument using a 2 × 150 bp paired end library and assembled into 29 scaffolds. The genome size is 7,077,367 nucleotides, with a GC content of 60.9 %. The final, high-quality draft genome contains 6693 protein coding genes, close to 85 % of which were assigned to COG categories. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRXL00000000. The sequencing of this genome will enable identification of genetic traits associated with host compatibility and high N2 fixation characteristics in Rhizobium leguminosarum. The sequence will also be useful for development of strain-specific markers to assess factors associated with environmental fitness, competiveness for host nodule occupancy, and survival on legume seeds (New Zealand Ministry of Business, Innovation and Employment program, ‘Improving forage legume-rhizobia performance’ contract C10X1308 and DairyNZ Ltd.).
Ultrasonic velocity and attenuation anisotropy of shales, Whitby, United Kingdom
Authors: Zhubayev Alimzhan , Houben Maartje E. , Smeulders David M. J. , Barnhoorn Auke 
Published: December 2015

We have conducted ultrasonic experiments, between 0.3 and 1 MHz, to measure velocity and attenuation ([Formula: see text]) anisotropy of P- and S-waves in dry Whitby Mudstone samples as a function of stress. We found the degree of anisotropy to be as large as 70% for velocity and attenuation. The sensitivity of P-wave anisotropy change with applied stress is more conspicuous than for S-waves. The closure of large aspect-ratio pores (and/or micro cracks) seems to be a dominant mechanism controlling the change of anisotropy. Generally, the highest attenuation is perceived for samples that have their bed layering perpendicular ([Formula: see text]) to the wave path. The observed attenuation in the samples is partly due to the scattering on the different layers, and it is partly due to the intrinsic attenuation. Changes in attenuation due to crack closure during the loading stage of the experiment are an indication of the intrinsic attenuation. The remaining attenuation can then be attributed to the layer scattering. Finally, the changes in attenuation anisotropy with applied stress are more dynamic with respect to changes in velocity anisotropy, supporting the validity of a higher sensitivity of attenuation to rock property changes.
High-Fat Diet and Palmitate Alter the Rhythmic Secretion of Glucagon-Like Peptide-1 by the Rodent L-cell
Authors: Gil-Lozano Manuel , Wu W. Kelly , Martchenko Alexandre , Brubaker Patricia L. 
Published: December 2015

Abstract
Secretion of the incretin hormone, glucagon-like peptide-1 (GLP-1), by the intestinal L-cell is rhythmically regulated by an independent molecular clock. However, the impact of factors known to affect the activity of similar cell-autonomous clocks, such as circulating glucocorticoids and high-fat feeding, on GLP-1 secretory patterns remains to be elucidated. Herein the role of the endogenous corticosterone rhythm on the pattern of GLP-1 and insulin nutrient-induced responses was examined in corticosterone pellet-implanted rats. Moreover, the impact of nutrient excess on the time-dependent secretion of both hormones was assessed in rats fed a high-fat, high-sucrose diet. Finally, the effects of the saturated fatty acid, palmitate, on the L-cell molecular clock and GLP-1 secretion were investigated in vitro using murine GLUTag L-cells. Diurnal variations in GLP-1 and insulin nutrient-induced responses were maintained in animals lacking an endogenous corticosterone rhythm, suggesting that glucocorticoids are not the predominant entrainment factor for L-cell rhythmic activity. In addition to hyperglycemia, hyperinsulinemia, insulin resistance, and disorganization of feeding behavior, high-fat high-sucrose-fed rats showed a total abrogation of the diurnal variation in GLP-1 and insulin nutrient-induced responses, with comparable levels of both hormones at the normal peak (5:00 pm) and trough (5:00 am) of their daily pattern. Finally, palmitate incubation induced profound derangements in the rhythmic expression of circadian oscillators in GLUTag L-cells and severely impaired the secretory activity of these cells. Collectively our findings demonstrate that obesogenic diets disrupt the rhythmic activity of the L-cell, partially through a direct effect of specific nutritional components.
DEVELOPMENT OF VERY LOW-LEVEL RADIOACTIVE WASTE SEQUESTRATION PROCESS CRITERIA
Authors: Chan Nicholas , Wong Pierre 
Published: December 2015

Segregating radioactive waste at the source and reclassifying radioactive waste to lower waste classes are the key activities to reduce the environmental footprint and long-term liability. In the Canadian Standards Association’s radioactive waste classification system, there are 2 sub-classes within low-level radioactive waste: very short-lived radioactive waste and very low-level radioactive waste (VLLW). VLLW has a low hazard potential but is above the Canadian unconditional clearance criteria as set out in Schedule 2 of Nuclear Substances and Devices Regulations. Long-term waste management facilities for VLLW do not require a high degree of containment and isolation. In general, a relatively low-cost near-surface facility with limited regulatory control is suitable for VLLW. At Canadian Nuclear Laboratories’ Chalk River Laboratories site an initiative, VLLW Sequestration, was implemented in 2013 to set aside potential VLLW for temporary storage and to be later dispositioned in the planned VLLW facility. As of May 2015, a total of 236 m3 resulting in approximately $1.1 million in total savings have been sequestered. One of the main hurdles in implementing VLLW Sequestration is the development of process criteria. Waste Acceptance Criteria (WAC) are used as a guide or as requirements for determining whether waste is accepted by the waste management facility. Establishment of the process criteria ensures that segregated waste materials have a high likelihood to meet the VLLW WAC and be accepted into the planned VLLW facility. This paper outlines the challenges and various factors which were considered in the development of interim process criteria.
Perceived changes to swallowing in people with Parkinson's disease
Authors: Noble Emma , Jones Diana , Miller Nick 
Published: December 2015

Aims: To describe the relationship between perceived swallowing difficulties in people with Parkinson's disease with disease-specific measures, carers' perceptions of swallowing problems and control participant responses. Method: In total, 119 people with Parkinson's disease, their carers and matched controls answered a specifically prepared functional swallowing changes questionnaire. People with Parkinson's completed a water-swallowing test. Motor status, cognition and mood were also assessed. Results: People with Parkinson's disease scored poorer than controls (p < 0.001) on the questionnaire. Those reporting swallowing problems scored poorer than those who did not (p < 0.01). However, of 74 people with Parkinson's who perceived no swallowing problem, 32 fell below the 50th percentile on the water-swallowing test, while 41 fell below cut-off scores on the questionnaire. There was a weak correlation between drinking speeds and questionnaire totals. Carers' evaluations correlated with their partners' (r = 0.796) but they perceived changes as less severe (p < 0.03). Conclusions: Despite reduced swallowing efficiency, some people with Parkinson's can successfully compensate. A sizeable proportion, however, remain unaware of swallowing problems when asked simply ‘Do you have a problem?’, but indicate difficulties with more specific questions. The present questionnaire provides a guide for gaining such insights for diagnosis, education and outcomes.
EDUCAÇÃO PARA O MUNDO DO TRABALHO: ALIENAÇÃO DA FUNÇÃO SOCIAL DA ATIVIDADE PRODUTIVA NA FORMAÇÃO DA JUVENTUDE
Authors: Marino Filho Armando 
Published: December 2015

O objetivo desse texto é analisar a formação da consciência na atividade educativa para o trabalho, segundo observações feitas em projetos de pesquisa que foram realizados no ano de 2014. Tem como foco a formação do pensamento sobre a realidade social e sua produção pelos homens. Fundamenta-se, na concepção materialista, histórica e dialética e toma como eixo a formação da consciência e da alienação no processo educativo, compreendendo a juventude na unidade que vai da infância à vida adulta e analisando implicações da prática educativa para o pensamento na atividade produtiva. As análises giram em torno de como se forma uma concepção sobre o trabalho no jovem, e como essa forma de pensar permanece no sujeito trabalhador adulto. Considera-se, ao final, que um tipo específico de pensamento fundamentado na crença tem orientado esses sujeitos na compreensão da realidade sobre a vida social. http://dx.doi.org/10.14572/nuances.v26i1.3820
On the probability of coincidence of cycle lengths for independent random permutations with given number of cycles
Authors: Timashev Aleksandr N. 
Published: December 2015

AbstractFrom the set of all permutations of the degree n with a given number N ≤ n of cycles two permutations are choosed randomly, uniformly and independently. The cycles of each permutation are numbered in some of N! possible ways. We study the coincidence probability of the cycle lengths of permutations for a given numbering. This probability up to a suitably selected renumbering of cycles of the first permutation equals to the probability of similarity of these permutations. The asymptotic estimates of the coincidence probability of the cycle lengths are obtained for five types of relations between N, n → ∞.
Pythagorean-hodograph cycloidal curves
Authors: Kozak Jernej , Krajnc Marjeta , Rogina Mladen , Vitrih Vito 
Published: December 2015

AbstractIn the paper, Pythagorean-hodograph cycloidal curves as an extension of PH cubics are introduced. Their properties are examined and a constructive geometric characterization is established. Further, PHC curves are applied in the Hermite interpolation, with closed form solutions been determined. The asymptotic approximation order analysis carried out indicates clearly which interpolatory curve solution should be selected in practice. This makes the curves introduced here a useful practical tool, in particular in algorithms that guide CNC machines.
Diagnostics and importance of hepatitis E virus infections
Authors: Osterman Andreas , Nitschko Hans , Eberle Josef , Campe Hartmut 
Published: December 2015

Abstract
The diagnosis of hepatitis E virus (HEV) infections has been recently substantially facilitated by the introduction of a whole range of new different virological assays. The increasing appearance of sporadic cases of acute hepatitis E in Germany directed the focus toward the zoonotic transmission route of the virus. The recognition of HEV genotypes differing in virulence and in pathogenic potential is not only relevant for epidemiology and the course of the disease, but also for the development and choice of diagnostic tools. A broad variety of enzymatic and protein-based assay formats detecting anti-HEV IgG or IgM antibodies directed against the different genotype variants of HEV is available (ELISA, LIA, Western blot); however, sensitivity and specificity of these assays differ notably. Today’s state-of-the art technology that permits fast and reliable assay-based confirmation of HEV infections is PCR. The newly developed commercially available PCR kits will detect all four human pathogenic HEV genotypes. Further subdivision and discrimination can be achieved by sequencing, although this approach is only reasonable in the setting of specific epidemiological demands. Detection of viral antigens, cell culture, and T-cell assays are of no practical importance in a routine diagnostic setting. New insight into the pathogenesis and its clinical relevance for defined groups of patients (immunosuppressed) as well as the implementation of specific antiviral and prophylactic therapies (vaccination) will further challenge the performance of existing assay formats and increase the technical demands for the diagnostic laboratory.
Choosing to Intervene: US Domestic Politics and Moral Imperatives
Authors: Haar Roberta , Krebs Lutz F. 
Published: December 2015

AbstractThe end of the Cold War meant fewer constraints on humanitarian intervention, and the third pillar of the nascent R2P norm suggests at least a moral imperative to intervene when another country’s population is threatened. Yet US leaders continue to shy away from protecting innocents outside of the United States from harm — despite the fact that presidential candidates often campaign on restoring America’s moral lead in the world and, in particular, on US responsibilities to avert mass atrocities. This paper investigates the extent to which US military intervention abroad is driven by domestic considerations. Using logistic regression analysis, we aim to explain decisions by Presidents Bush Sr., Clinton and Bush Jr. to send troops into harms way.
In-mould graining of 3D-shaped parts with micro-structured surfaces
Authors: Hopmann Christian , Martens Jonathan 
Published: December 2015

Abstract
To make functional surface effects on plastic components usable on an industrial scale, new processes have to be developed. The in-mould graining (IMG) process is a variant of the thermoforming enabling the manufacture of micro-structures. One advantage of the IMG process is that the surface structure is formed to the final shape of the part. Recent investigations show that the forming accuracy of a flat mould can be extended by applying a variothermal mould heating in the IMG process. As high mould temperatures lead to high forming accuracies but also increase the cycle time of the process, a conflict of interests follows. In this paper, it is investigated to what extent high moulding accuracies can be achieved to form three-dimensional parts by using the IMG process extended by a variothermal mould heating. The results show that forming accuracies of micro-scaled structures of >90% can be reached on the bottom and the side walls of a cup-shaped mould. To address the conflict of interests between high moulding accuracy and long cycle times, the influence of the mould temperature is investigated in detail. To analyse the micro-forming behaviour of the processed material, analyses of the rheological behaviour with a rotational rheometer are carried out.
The difference between electrical microstimulation and direct electrical stimulation – towards new opportunities for innovative functional brain mapping?
Authors: Vincent Marion , Rossel Olivier , Hayashibe Mitsuhiro , Herbet Guillaume , Duffau Hugues , Guiraud David , Bonnetblanc François 
Published: December 2015

AbstractBoth electrical microstimulation (EMS) and direct electrical stimulation (DES) of the brain are used to perform functional brain mapping. EMS is applied to animal fundamental neuroscience experiments, whereas DES is performed in the operating theatre on neurosurgery patients. The objective of the present review was to shed new light on electrical stimulation techniques in brain mapping by comparing EMS and DES. There is much controversy as to whether the use of DES during wide-awake surgery is the ‘gold standard’ for studying the brain function. As part of this debate, it is sometimes wrongly assumed that EMS and DES induce similar effects in the nervous tissues and have comparable behavioural consequences. In fact, the respective stimulation parameters in EMS and DES are clearly different. More surprisingly, there is no solid biophysical rationale for setting the stimulation parameters in EMS and DES; this may be due to historical, methodological and technical constraints that have limited the experimental protocols and prompted the use of empirical methods. In contrast, the gap between EMS and DES highlights the potential for new experimental paradigms in electrical stimulation for functional brain mapping. In view of this gap and recent technical developments in stimulator design, it may now be time to move towards alternative, innovative protocols based on the functional stimulation of peripheral nerves (for which a more solid theoretical grounding exists).
A semi-Lagrangian method on dynamically adapted octree meshes
Authors: Terekhov Kirill M. , Nikitin Kirill D. , Olshanskii Maxim A. , Vassilevski Yuri V. 
Published: December 2015

AbstractThe paper develops a semi-Lagrangian method for the numerical integration of the transport equation discretized on adaptive Cartesian cubic meshes. We use dynamically adaptive graded Cartesian grids. They allow for a fast grid reconstruction in the course of numerical integration. The suggested semi- Lagrangian method uses a higher order interpolation with a limiting strategy and a back-and-forth correction of the numerical solution. The interpolation operators have compact nodal stencils. In a series of experiments with dynamically adapted meshes, we demonstrate that the method has at least the second-order convergence and acceptable conservation and monotonicity properties.
1,3,5-Tris[(trimethylstannyl)ethynyl]- 1,3,5-trimethyl-1,3,5-trisilacyclohexane
Authors: Weisheim Eugen , Stammler Hans-Georg , Mitzel Norbert W. 
Published: December 2015

Abstract
The reaction of 1,3,5-triethynyl-1,3,5-trimethyl- 1,3,5-trisilacyclohexane with (dimethylamino)trimethylstannane afforded 1,3,5-tris[(trimethylstannyl)ethynyl]- 1,3,5-trimethyl-1,3,5-trisilacyclohexane with tin-functionalised ethynyl groups. The compound was characterized by single-crystal X-ray diffraction, elemental analysis, mass spectrometry, NMR and FT-IR spectroscopy.