bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low-Pathogenic Influenza A Viruses in North American Diving Ducks Contribute to the Emergence of a Novel Highly Pathogenic Influenza A(H7N8) Virus
Authors: Xu Yifei , Ramey Andrew M. , Bowman Andrew S. , DeLiberto Thomas J. , Killian Mary L. , Krauss Scott , Nolting Jacqueline M. , Torchetti Mia Kim , Reeves Andrew B. , Webby Richard J. , Stallknecht David E. , Wan Xiu-Feng 
Published: February 2017

ABSTRACT
Introductions of low-pathogenic avian influenza (LPAI) viruses of subtypes H5 and H7 into poultry from wild birds have the potential to mutate to highly pathogenic avian influenza (HPAI) viruses, but such viruses' origins are often unclear. In January 2016, a novel H7N8 HPAI virus caused an outbreak in turkeys in Indiana, USA. To determine the virus's origin, we sequenced the genomes of 441 wild-bird origin influenza A viruses (IAVs) from North America and subjected them to evolutionary analyses. The results showed that the H7N8 LPAI virus most likely circulated among diving ducks in the Mississippi flyway during autumn 2015 and was subsequently introduced to Indiana turkeys, in which it evolved high pathogenicity. Preceding the outbreak, an isolate with six gene segments (PB2, PB1, PA, HA, NA, and NS) sharing >99% sequence identity with those of H7N8 turkey isolates was recovered from a diving duck sampled in Kentucky, USA. H4N8 IAVs from other diving ducks possessed five H7N8-like gene segments (PB2, PB1, NA, MP, and NS; >98% sequence identity). Our findings suggest that viral gene constellations circulating among diving ducks can contribute to the emergence of IAVs that affect poultry. Therefore, diving ducks may serve an important and understudied role in the maintenance, diversification, and transmission of IAVs in the wild-bird reservoir.

IMPORTANCE
In January 2016, a novel H7N8 HPAI virus caused a disease outbreak in turkeys in Indiana, USA. To determine the origin of this virus, we sequenced and analyzed 441 wild-bird origin influenza virus strains isolated from wild birds inhabiting North America. We found that the H7N8 LPAI virus most likely circulated among diving ducks in the Mississippi flyway during autumn 2015 and was subsequently introduced to Indiana turkeys, in which it evolved high pathogenicity. Our results suggest that viral gene constellations circulating among diving ducks can contribute to the emergence of IAVs that affect poultry. Therefore, diving ducks may play an important and understudied role in the maintenance, diversification, and transmission of IAVs in the wild-bird reservoir. Our study also highlights the importance of a coordinated, systematic, and collaborative surveillance for IAVs in both poultry and wild-bird populations.
Coronary heart disease is associated with a worse clinical outcome of hand osteoarthritis: a cross-sectional and longitudinal study
Authors: Courties Alice , Sellam Jérémie , Maheu Emmanuel , Cadet Christian , Barthe Yoann , Carrat Fabrice , Berenbaum Francis 
Published: February 2017

ObjectiveTo determine whether cardiometabolic factors are associated with hand osteoarthritis (HOA) symptoms, radiographic severity and progression in a post hoc analysis of the phase III Strontium ranelate Efficacy in Knee OsteoarthrItis triAl (SEKOIA) trial, designed to determine the effect of strontium ranelate on knee osteoarthritis (OA).MethodsAmong the 1683 patients randomised in the SEKOIA study, 869 with radiographic HOA at baseline (rHOA≥2 joints with Kellgren-Lawrence grade ≥2) were included in a cross-sectional analysis. For longitudinal study, we included only the 307 patients with rHOA at baseline from the placebo group. We evaluated whether baseline symptomatic HOA, radiographic severity and clinical and rHOA progression were associated with coronary heart disease and/or metabolic diseases (obesity, diabetes and hypertension, dyslipidaemia) by multivariate regression analysis.ResultsAt baseline, 869 patients (72% women) were included in the cross-sectional analysis; 26% were symptomatic. On multivariate analysis, symptomatic HOA was associated with coronary heart disease (OR 3.59, 95% CI (1.78 to 7.26)) but not metabolic diseases. After a mean follow-up of 2.6 years, for the 307 participants in the placebo group, on multivariate analysis, worse clinical HOA outcome was associated with coronary heart disease (OR 2.91, 95% CI (1.02 to 8.26)). The slow radiographic progression did not allow for revealing any associated factors.ConclusionsSymptomatic HOA and worse HOA clinical course are associated with coronary heart disease. These results strengthen the systemic component of HOA and the association between OA pain and cardiac events.Trial registration numberISRCTN41323372.
Functional Outcomes of Antegrade Homodigital Neurovascular Island Flaps for Fingertip Amputation
Authors: Lok Lin-Wing , Chan Wing-Leung , Lau Yan-Kit 
Published: February 2017

Background: Fingertip amputations can be debilitating and challenging to treat, due to the need for both function and cosmesis. We studied the functional outcomes of patients receiving an antegrade homodigital neurovascular island flap for fingertip amputations. Methods: From 2004 to 2013, we performed 13 cases of antegrade homodigital neurovascular island flaps. Functional outcomes were studied. Four had Allen Zone II and nine had Zone III injuries. Seven were performed as secondary surgery. The mean followup period was 36 weeks. Results: All the flaps survived. Ten of 11 had less than or equal to 6 mm two-point discrimination. Five patients were noted to have mild flexion contracture at distal interphalangeal joints ranging from 5 to 10 degrees. There were three cases with nail deformity, three with fingertip hypersensitivity and four with cold intolerance. For patients injured on duty, the mean duration of sick leave taken was 18.5 weeks. Conclusions: With careful patient selection, the antegrade homodigital neurovascular island flap is a reliable means of restoring the function of the fingertip after amputation injury with significant tissue loss. Flap survival, sensibility and mobile adjacent joint can be expected, even when performed as a secondary surgery and for patients with work-related injuries.
Trigonometry-Integrated ‘Lift’ Technique (TILT) for Restoring Volar Tilt in Distal Radius Fractures: Description of Technique and Preliminary Results
Authors: Sechachalam Sreedharan , Satku Mala , Wong Jian Hao Kevin , Tan Lester Teong Jin , Yong Fok Chuan 
Published: February 2017

Background: Restoration of extra-articular and intra-articular parameters are important considerations during operative fixation of distal radius fractures. Restoration of volar tilt by using visual estimation and the ‘lift’ technique has previously been described. The aim of our study was to describe a mathematical technique for accurately restoring the volar tilt of the distal radius to acceptable anatomic values. Methods: A retrospective review of cases performed using the trigonometry-integrated ‘ lift’ technique (TILT) was performed. This technique uses the pre-operative volar tilt angle as well as the dimensions of the implant to calculate the ‘lift’ required to restore volar tilt. Intra-operative angles were measured using a marked transparency overlay on fluoroscopic images. Pre-operative and post-operative volar tilt were measured and analysed. Results: Twenty-seven fractures were included in the study, with 20 being classified as Arbeitsgemeinschaft für Osteosynthesefragen (AO) C-type. Pre-‘lift’ volar tilt ranged from 0° to -20°. Post-‘lift’ volar tilt ranged from 2° to 16°, with all but three cases ranging from 5° to 15°. The mean volar tilt achieved was 10.2°. Conclusions: The trigonometry-integrated ‘lift’ technique resulted in reliable intra-operative restoration of anatomic volar tilt in distal radius fractures.
A Biomechanical Comparison of Different Knots Tied on Fibrewire Suture
Authors: Jiang Jackson , Mat Jais Ita Suzana , Yam Andrew Kean Tuck , McGrouther Duncan Angus , Tay Shian Chao 
Published: February 2017

Background: Synthetic sutures such as Fiberwire used in flexor tendon repairs have high tensile strength. Proper application allows early mobilisation, decreasing morbidity from repair rupture and adhesions while preserving range of motion. Suture stiffness can cause poorer knot holding, contributing to gapping, peritendinous adhesions or rupture. Previous studies recommended more throws in knots tied on Fiberwire to prevent knot slippage. These larger knots are voluminous and prominent. In tendon repairs they can cause “catching”, increase friction and work of flexion. Other studies advocated certain complicated knots as being more secure. We evaluated several knots and their biomechanical properties with the aim of finding a compact knot with less potential for slippage to maximise strength potential of flexor tendon repairs using Fiberwire. Methods: A series of different knots tied on Fiberwire 4-0 sutures were pulled to failure on a mechanical tester. Mean tensile strengths, knot volumes and tensile strength to knot volume ratios were compared. Results: Tensile strengths and knot volume increased with more throws and loops. Four variations of the square knot (the 4=4=1, 2=2=2=2, 1=1=1=1=1, 2=1=1=1=1 knots) had tensile strengths greater than 35N. The specialised anti-slip knot had highest tensile strength and suture volume but lower strength-to-volume ratio. Conclusions: The anti-slip knot had highest tensile strength but it also had the highest volume. The greater strength of repair may not translate into improved clinical outcome. The 1=1=1=1=1 knot has superior knot strength-to-volume ratio with good knot strength adequate for early active mobilisation in flexor tendon repairs.
Distinct Levels of Radioresistance in Lgr5+ Colonic Epithelial Stem Cells versus Lgr5+ Small Intestinal Stem Cells
Authors: Hua Guoqiang , Wang Chu , Pan Yan , Zeng Zhaoshi , Lee Sang Gyu , Martin Maria Laura , Haimovitz-Friedman Adriana , Fuks Zvi , Paty Philip B. , Kolesnick Richard 
Published: February 2017

Abstract
Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC) markedly more radioresistant in vivo than small intestinal crypt base columnar stem cells (CBC; D0 = 6.0 ± 0.3 Gy vs. 1.3 ± 0.1, respectively; P &lt; 0.01). Accordingly, CESCs survived 30 Gy exposure, while CBCs were completely depleted after 15 Gy. EdU incorporation studies indicated that after 19 Gy, CBCs exited growth arrest at 12 hours, resuming normal mitotic activity despite 60% of this population displaying residual γH2AX foci, indicative of persistent unrepaired DNA damage. Checkpoint recovery before complete double-strand break (DSB) repair represents the sine qua non of a newly defined potentially lethal pathophysiology termed checkpoint adaptation. In the small intestinal mucosa, checkpoint adaptation resulted in CBCs succumbing to an 8-fold increase in the incidence of highly lethal chromosomal aberrations and mitotic catastrophe by 48 hours postradiation. In contrast, Lgr5+ CESCs displayed delayed checkpoint recovery at 48 hours post-19 Gy, coordinated with complete DSB repair and regeneration of colonic mucosa originating, at least in part, from surviving CESCs. The discovery that small intestinal CBCs succumb to checkpoint adaptation is the first demonstration that this aberrant cell-cycle response may drive mammalian tissue radiosensitivity. Cancer Res; 77(8); 2124–33. ©2017 AACR.
Therapeutic Potential of Bacteria against Solid Tumors
Authors: Hatzikirou Haralampos , López Alfonso Juan Carlos , Leschner Sara , Weiss Siegfried , Meyer-Hermann Michael 
Published: February 2017

Abstract
Intentional bacterial infections can produce efficacious antitumor responses in mice, rats, dogs, and humans. However, low overall success rates and intense side effects prevent such approaches from being employed clinically. In this work, we titered bacteria and/or the proinflammatory cytokine TNFα in a set of established murine models of cancer. To interpret the experiments conducted, we considered and calibrated a tumor–effector cell recruitment model under the influence of functional tumor-associated vasculature. In this model, bacterial infections and TNFα enhanced immune activity and altered vascularization in the tumor bed. Information to predict bacterial therapy outcomes was provided by pretreatment tumor size and the underlying immune recruitment dynamics. Notably, increasing bacterial loads did not necessarily produce better long-term tumor control, suggesting that tumor sizes affected optimal bacterial loads. Short-term treatment responses were favored by high concentrations of effector cells postinjection, such as induced by higher bacterial loads, but in the longer term did not correlate with an effective restoration of immune surveillance. Overall, our findings suggested that a combination of intermediate bacterial loads with low levels TNFα administration could enable more favorable outcomes elicited by bacterial infections in tumor-bearing subjects. Cancer Res; 77(7); 1553–63. ©2017 AACR.
How Truncating Are ‘Truncating Languages'? Evidence from Russian and German
Authors: Rathcke Tamara V. 
Published: February 2017

AbstractRussian and German have pr eviously been described as ‘truncating‘, or cutting off target frequencies of the phrase-final pitch trajectories when the time available for voicing is compromised. However, supporting evidence is rare and limited to only a few pitch categories. This paper reports a production study conducted to document pitch adjustments to linguistic materials, in which the amount of voicing available for the realization of a pitch pattern varies from relatively long to extremely short. Productions of nuclear H+L*, H* and L*+H pitch accents followed by a low boundary tone were investigated in the two languages. The results of the study show that speakers of both ‘truncating languages' do not utilize truncation exclusively when accommodating to different segmental environments. On the contrary, they employ several strategies - among them is truncation but also compression and temporal re-alignment - to produce the target pitch categories under increasing time pressure. Given that speakers can systematically apply all three adjustment strategies to produce some pitch patterns (H* L% in German and Russian) while not using truncation in others (H+L* L% particularly in Russian), we question the effectiveness of the typological classification of these two languages as ‘truncating'. Moreover, the phonetic detail of truncation varies considerably, both across and within the two languages, indicating that truncation cannot be easily modeled as a unified phenomenon. The results further suggest that the phrase-final pitch adjustments are sensitive to the phonological composition of the tonal string and the status of a particular tonal event (associated vs. boundary tone), and do not apply to falling vs. rising pitch contours across the board, as previously put forward for German. Implications for the intonational phonology and prosodic typology are addressed in the discussion.
Stability and Variability in Slovak Prosodic Boundaries
Authors: Benuš Štefan , Šimko Juraj 
Published: February 2017

AbstractBackground/Aim: Encoding intended meanings in the type and strength of prosodic boundaries and strategies for communicating these meanings in ambient noise use similar prosodic cues. We analyze how increasing the level of ambient noise affects the realization of Slovak prosodic boundaries. Methods: Five native speakers of Slovak read sentences, manipulating the boundary type (weak, rise, fall) and the location of pre-boundary pitch accent. Ambient noise of several levels was administered via headphones. Acoustic and articulatory data (electromagnetometry) were collected. Results: Under normal condition, boundary strength is signaled with longer pre-boundary rhymes, more frequent pauses, greater crossboundary f0 resets and jaw displacement. The strength of falls is realized in crossboundary features (pauses, f0 reset), and rises in pre-boundary features (rhyme duration, f0 range). Pitch-accented rhymes are strengthened in all features, but f0 range. In noise, the increase in boundary strength is weak, and falls strengthen more than rises. F0 targets for falls and rises are adjusted in addition to noiseinduced global f0 scaling and lengthening. Conclusion: Hyper-articulation of prosodic boundaries in ambient noise is not robust and uniform; rather, durational, f0 and jaw displacement features co-create complex prosodic patterns in a complementary and synergetic manner based on affordances in normal speech.
JAM-C maintains VEGR2 expression to promote retinal pigment epithelium cell survival under oxidative stress
Authors: Jia Xin , Zhao Chen , Chen Qishan , Du Yuxiang , Huang Lijuan , Ye Zhimin , Ren Xiangrong , Wang Shasha , Lee Chunsik , Tang Zhongshu , Li Xuri , Ju Rong 
Published: February 2017

SummaryJunctional adhesion molecule-C (JAM-C) has been shown to play critical roles during development and in immune responses. However, its role in adult eyes under oxidative stress remains poorly understood. Here, we report that JAM-C is abundantly expressed in adult mouse retinae and choroids in vivo and in cultured retinal pigment epithelium (RPE) and photoreceptor cells in vitro. Importantly, both JAM-C expression and its membrane localisation are downregulated by H2O2-induced oxidative stress. Under H2O2-induced oxidative stress, JAM-C is critically required for the survival of human RPE cells. Indeed, loss of JAM-C by siRNA knockdown decreased RPE cell survival. Mechanistically, we show that JAM-C is required to maintain VEGFR2 expression in RPE cells, and VEGFR2 plays an important role in keeping the RPE cells viable since overexpression of VEGFR2 partially restored impaired RPE survival caused by JAM-C knockdown and increased RPE survival. We further show that JAM-C regulates VEGFR2 expression and, in turn, modulates p38 phosphorylation. Together, our data demonstrate that JAM-C plays an important role in maintaining VEGR2 expression to promote RPE cell survival under oxidative stress. Given the vital importance of RPE in the eye, approaches that can modulate JAM-C expression may have therapeutic values in treating diseases with impaired RPE survival.
Small but mighty: Platelets as central effectors of host defense
Authors: Hellen Ishikawa-Ankerhold , Steffen Massberg , Stocker Thomas , Christian Schulz 
Published: February 2017

SummaryPlatelets actively participate in inflammatory processes and drive diseases such as atherosclerosis, rheumatoid arthritis and cancer metastasis. However, platelets also have anti-inflammatory and anti-infective properties, which have received less consideration in the past. In this review, we highlight recent findings on the role of platelets in host defense and describe regulatory pathways modulating immuneresponses. Furthermore, we discuss the role of platelets for the resolution of inflammation and tissue repair. These conceptual changes contribute to our understanding of platelet biology in disease.
A Comparison between the GPM Dual-Frequency Precipitation Radar and Ground-Based Radar Precipitation Rate Estimates in the Swiss Alps and Plateau
Authors: Speirs Peter , Gabella Marco , Berne Alexis 
Published: February 2017

Abstract
The Global Precipitation Measurement (GPM) mission Dual-Frequency Precipitation Radar (DPR) provides a unique set of three-dimensional radar precipitation estimates across much of the globe. Both terrain and climatic conditions can have a strong influence on the reliability of these estimates. Switzerland provides an ideal testbed to evaluate the performance of the DPR in complex terrain: it consists of a mixture of very complex terrain (the Alps) and the far flatter Swiss Plateau. It is also well instrumented, covered with a dense gauge network as well as a network of four dual-polarization C-band weather radars, with the same instrument network used in both the Plateau and the Alps. Here an evaluation of the GPM DPR rainfall rate products against the MeteoSwiss radar rainfall rate product for the first two years of the GPM DPR’s operation is presented. Errors in both detection and estimation are considered, broken down by terrain complexity, season, precipitation phase, precipitation type, and precipitation rate. Errors are considered both integrated across the entire domain and spatially, and consistent underestimation of precipitation by GPM is found. This rises to −51% in complex terrain in the winter, primarily due to the predominance of DPR measurements wholly in the solid phase, where problems are caused by lower reflectivities. The smaller vertical extent of precipitation in winter is also likely a cause. Both detection and estimation performance are found to be significantly better in summer than in winter, in liquid than in solid precipitation, and in flatter terrain than in complex terrain.
GEFS Precipitation Forecasts and the Implications of Statistical Downscaling over the Western United States
Authors: Lewis Wyndam R. , Steenburgh W. James , Alcott Trevor I. , Rutz Jonathan J. 
Published: February 2017

Abstract
Contemporary operational medium-range ensemble modeling systems produce quantitative precipitation forecasts (QPFs) that provide guidance for weather forecasters, yet lack sufficient resolution to adequately resolve orographic influences on precipitation. In this study, cool-season (October–March) Global Ensemble Forecast System (GEFS) QPFs are verified using daily (24 h) Snow Telemetry (SNOTEL) observations over the western United States, which tend to be located at upper elevations where the orographic enhancement of precipitation is pronounced. Results indicate widespread dry biases, which reflect the infrequent production of larger 24-h precipitation events (≳22.9 mm in Pacific ranges and ≳10.2 mm in the interior ranges) compared with observed. Performance metrics, such as equitable threat score (ETS), hit rate, and false alarm ratio, generally worsen from the coast toward the interior. Probabilistic QPFs exhibit low reliability, and the ensemble spread captures only ~30% of upper-quartile events at day 5. In an effort to improve QPFs without exacerbating computing demands, statistical downscaling is explored based on high-resolution climatological precipitation analyses from the Parameter-Elevation Regressions on Independent Slopes Model (PRISM), an approach frequently used by operational forecasters. Such downscaling improves model biases, ETSs, and hit rates. However, 47% of downscaled QPFs for upper-quartile events are false alarms at day 1, and the ensemble spread captures only 56% of the upper-quartile events at day 5. These results should help forecasters and hydrologists understand the capabilities and limitations of GEFS forecasts and statistical downscaling over the western United States and other regions of complex terrain.