bluuebunny/crossref_metadata_2025_split huggingface dataset:
Palmar Eccrine Hidradenitis Secondary to Trauma from Computer Gaming in an Adolescent After Bone Marrow Transplantation
Authors: Lee Lynette Ying , Koh Mark Jean‐Aan 
Published: August 2017

AbstractA 14‐year‐old boy who had undergone a matched sibling bone marrow transplant for acute lymphoblastic leukemia presented with painful nodules on his palms after prolonged gaming on his computer and mobile phone. Histology showed a neutrophilic inflammatory infiltrate surrounding the acrosyringium and eccrine sweat coils in the deep dermis. The lesions resolved spontaneously with conservative management.
Assessment of the inheritance of resistance and tolerance in cherry (Prunus sp.) to Blumeriella jaapii, the causal agent of cherry leaf spot
Authors: Andersen K. L. , Sebolt A. M. , Sundin G. W. , Iezzoni A. F. 
Published: August 2017

Cherry leaf spot (CLS), caused by Blumeriella jaapii, is a serious fungal disease of sour cherry (Prunus cerasus). Cultivar Montmorency, the major cultivar grown in the United States, is highly susceptible to CLS. As many as 10 fungicide sprays can be required each growing season to combat this disease; therefore, developing CLS‐resistant cultivars is a top breeding priority. Germplasm previously reported to be resistant or tolerant to CLS was acquired and incorporated into the sour cherry breeding programme at Michigan State University (MSU) and included three cherry species: sour cherry, sweet cherry (P. avium), and the wild species P. canescens. This study aimed to: (i) compare the CLS disease progression profile of the susceptible cultivar Montmorency with those of the resistant and tolerant germplasm; and (ii) gain an understanding of the inheritance of these resistance and tolerance traits by evaluating the host response of progeny individuals belonging to families derived from this germplasm. Significant differences were observed between the susceptible Montmorency and the tolerant and resistant accessions in their response to CLS and its progression during the growing season. Evaluation of the CLS host responses of progeny individuals derived from this germplasm supported a dominant two‐gene model for P. canescens‐derived resistance and a recessive gene model for sweet cherry‐derived tolerance. These insights into disease progression and trait inheritance improve the efficiency and potential success of breeding sour cherry cultivars with durable resistance to CLS.
Challenges in interprofessional collaboration: experiences of care providers and policymakers in a newly set‐up Dutch assault centre
Authors: Zijlstra Elza , Lo Fo Wong Sylvie , Teerling Anne , Hutschemaekers Giel , Lagro‐Janssen Antoine 
Published: August 2017

BackgroundSexual and family violence are problems that affect many women and men, and the negative health consequences of violence are numerous. As adequate acute interprofessional care can prevent negative health consequences and improve forensic medical examination, a Centre for Sexual and Family Violence was set up.AimWe aimed to improve our understanding of the challenges in interprofessional collaboration in a newly set‐up centre for sexual and family violence.MethodsWe conducted a qualitative study with semi‐structured interviews about the experiences with interprofessional collaboration of 16 stakeholders involved in the Centre for Sexual and Family Violence Nijmegen. Participants were selected by purposive sampling.ResultsParticipants found that the interprofessional collaboration had improved communication and competences. However, there were challenges too. Firstly, the interprofessional collaboration had brought parties closer together, but the collaboration also forced professionals to strongly define their boundaries. Mutual trust and understanding needed to be built up. Secondly, a balance had to be struck between pursuing the shared vision – which was to improve quality of care for victims – and giving space to organizations' and professionals' own interest. Thirdly, care for victims of sexual and family violence could be demanding on healthcare providers in an emotional sense, which might jeopardize professional's initial motivation for joining the Centre for Sexual and Family Violence Nijmegen.ConclusionThe interprofessional collaboration in an assault centre improves quality of care for victims, but there are also challenges. The tasks of an assault centre are to create opportunities to discuss professional roles and professional interests, to build up good interpersonal relations in which trust and understanding can grow, to formulate a strong and shared victim‐centred vision and to support care providers with training, feedback and supervision.
First‐order vs. second‐order structural validity of the Health Literacy Scale in patients with diabetes
Authors: Lee Eun‐Hyun , Lee Young Whee 
Published: August 2017

ObjectiveThe purpose of this study was to determine the structural validity of the Health Literacy Scale (HLS) in Korean patients with diabetes based on the previously reported first‐order three‐factor and two‐factor models and a newly proposed second‐order model, to identify which model best represents the structure of the Korean version of the HLS (HLS‐K).MethodsThe HLS was translated from Japanese into Korean using a translation and back‐translation technique. A secondary data analysis was used to validate the structure of the HLS. Data were obtained from a cross‐sectional survey involving 459 adults with diabetes recruited from outpatient clinics at two university hospitals in South Korea. The structural validity was examined using confirmatory factor analysis. Additionally, the known‐groups validity by education level and internal consistency validity were assessed.ResultsThe second‐order three‐factor model of the HLS‐K exhibited a good fit to the data, as indicated by χ2/df = 3.891, SRMR = 0.042, GFI = 0.924, RMSEA = 0.079 (90% CI = 0.069–0.090), and CFI = 0.962. The second‐order three‐factor model empirically demonstrated that both communicative and critical factors explained the variance in the overall health literacy better than did the functional factor. The HLS‐K mean score was significantly lower for patients with only elementary school education than for those with higher education levels, implying the presence of known‐groups validity. Cronbach's alpha for the total scale was 0.90.ConclusionsThis study found that the second‐order three‐factor model of the HLS‐K is better than that the original first‐order three‐factor and first‐order two‐factor models. Further validation studies are needed to generalise the underlying structure of the instrument in diabetes populations across various cultures.
SpectraCam®: A new polarized hyperspectral imaging system for repeatable and reproducible in vivo skin quantification of melanin, total hemoglobin, and oxygen saturation
Authors: Nkengne A. , Robic J. , Seroul P. , Gueheunneux S. , Jomier M. , Vie K. 
Published: August 2017

AbstractBackgroundAn accurate way to determine skin pigmentation is to acquire the spectral reflectance of a skin sample and to quantify chromophores by reverse calculation from physical models of light propagation. Therefore, we tested a new hyperspectral imaging device and software suite, the SpectraCam® system, and evaluated its accuracy to quantify skin chromophores.MethodsValidation of the SpectraCam® system was performed by, firstly, comparing the known and the acquired reflectance spectra of color phantoms. Repeatability and reproducibility were then evaluated by two operators who performed acquisitions at different time points and compared the acquired reflectance spectra. The specificity of the system was tested by quantitative analysis of single chromophore variation models: lentigo and pressure relief. Finally, we tested the ability of the SpectraCam® system to detect variations in chromophore in the eye region due to the daily application of a new anti‐dark circle cosmetic product.ResultsThe SpectraCam® system faithfully acquires the reflectance spectra of color phantoms (r2>0.90). The skin reflectance spectra acquired by different operators at different times are highly repeatable (r2>0.94) and reproducible (r2>0.99). The SpectraCam® system can also produce qualitative maps that reveal local variations in skin chromophore or underlying structures such as blood vessels. The system is precise enough to detect melanin variation in lentigo or total hemoglobin and oxygen saturation variations upon pressure relief. It is also sensitive enough to detect a decrease in melanin in the eye region due to the application of an anti‐dark circle cosmetic product.ConclusionThe SpectraCam® system proves to be rapid and produces high‐resolution data encompassing a large field of view. It is a robust hyperspectral imaging system that quantifies melanin, total hemoglobin, and oxygen saturation and is well adapted to cosmetic research.
In vivo measurements of blood vessels’ distribution in non‐melanoma skin cancer by dynamic optical coherence tomography — a new quantitative measure?
Authors: Sigsgaard V. , Themstrup L. , Theut Riis P. , Olsen J. , Jemec G. B. 
Published: August 2017

AbstractBackgroundSkin biopsies only provide point‐in‐time data making longitudinal studies difficult. Using Dynamic optical coherence tomography (D‐OCT) in vivo, non‐invasive imaging of the microvasculature becomes possible. The current OCT literature is almost exclusively based on qualitative information but quantitative data may offer additional advantages, for example, by reducing observer variation. The objective of this study was to evaluate the in vivo superficial distribution of blood vessels, defined as the surface‐to‐first‐vessel distance, in actinic keratosis, basal cell carcinoma, squamous cell carcinoma and normal skin.MethodsD‐OCT images of 52 histologically verified lesions and corresponding normal skin were included. The dataset consisted of 13 AK, 22 BCC and 17 SCC. No tumour sub‐classification was done.ResultsThe quantitative measurement of the surface‐to‐first‐vessel distance showed a significant difference between NMSC and corresponding normal skin. Significant differences were also seen for BCC, and it is hypothesized that the measure may also be able to distinguish between AK and SCC (borderline significant differences).ConclusionOur results could prove useful in discriminating between non‐melanoma skin cancer lesions and healthy skin and in making a specific diagnosis. It is suggested that collecting different quantitative measurements increases the utility of D–OCT and makes the method less observer‐dependent.
Macrodinychus tanduk sp. nov., an unusual new macrodinychid species from Sumatra, Indonesia (Mesostigmata Uropodina), with notes on the Macrodinychidae fam. nov.
Authors: Kontschán Jeno 
Published: August 2017

A new diagnosis and the type genus of Macrodinychidae fam. nov. are given with the description of a new and very extreme macrodinychid mite (Macrodinychus tanduk sp. nov.) from Sumatra, Indonesia. The two horn-like processes formed on the anterior region of idiosoma, a strongly sclerotized half-ring-like court situated posterior to genital opening and the large and semicircular pygidial shield are unusual character states within the family Macrodinychidae. A new, updated key to the macrodinychid species is given.
Prediction of the Maximum Number of Repetitions and Repetitions in Reserve From Barbell Velocity
Authors: García-Ramos Amador , Torrejón Alejandro , Feriche Belén , Morales-Artacho Antonio J. , Pérez-Castilla Alejandro , Padial Paulino , Haff Guy Gregory 
Published: August 2017

Purpose: To provide 2 general equations to estimate the maximum possible number of repetitions (XRM) from the mean velocity (MV) of the barbell and the MV associated with a given number of repetitions in reserve, as well as to determine the between-sessions reliability of the MV associated with each XRM. Methods: After determination of the bench-press 1-repetition maximum (1RM; 1.15 ± 0.21 kg/kg body mass), 21 men (age 23.0 ± 2.7 y, body mass 72.7 ± 8.3 kg, body height 1.77 ± 0.07 m) completed 4 sets of as many repetitions as possible against relative loads of 60%1RM, 70%1RM, 80%1RM, and 90%1RM over 2 separate sessions. The different loads were tested in a randomized order with 10 min of rest between them. All repetitions were performed at the maximum intended velocity. Results: Both the general equation to predict the XRM from the fastest MV of the set (CV = 15.8–18.5%) and the general equation to predict MV associated with a given number of repetitions in reserve (CV = 14.6–28.8%) failed to provide data with acceptable between-subjects variability. However, a strong relationship (median r2 = .984) and acceptable reliability (CV < 10% and ICC > .85) were observed between the fastest MV of the set and the XRM when considering individual data. Conclusions: These results indicate that generalized group equations are not acceptable methods for estimating the XRM–MV relationship or the number of repetitions in reserve. When attempting to estimate the XRM–MV relationship, one must use individualized relationships to objectively estimate the exact number of repetitions that can be performed in a training set.
Behavioral Effects of a Locomotor-Based Physical Activity Intervention in Preschoolers
Authors: Burkart Sarah , Roberts Jasmin , Davidson Matthew C. , Alhassan Sofiya 
Published: August 2017

Background: Poor adaptive learning behaviors (ie, distractibility, inattention, and disruption) are associated with behavior problems and underachievement in school, as well as indicating potential attention-deficit hyperactivity disorder. Strategies are needed to limit these behaviors. Physical activity (PA) has been suggested to improve behavior in school-aged children, but little is known about this relationship in preschoolers. This study examined the effects of a PA intervention on classroom behaviors in preschool-aged children. Methods:Eight preschool classrooms (n = 71 children; age = 3.8 ± 0.7 y) with children from low socioeconomic environments were randomized to a locomotor-based PA (LB-PA) or unstructured free playtime (UF-PA) group. Both interventions were implemented by classroom teachers and delivered for 30 minutes per day, 5 days per week for 6 months. Classroom behavior was measured in both groups at 3 time points, whereas PA was assessed at 2 time points over a 6-month period and analyzed with hierarchical linear modeling. Results:Linear growth models showed significant decreases in hyperactivity (LB-PA: −2.58 points, P = .001; UF-PA: 2.33 points, P = .03), aggression (LB-PA: −2.87 points, P = .01; UF-PA: 0.97 points, P = .38) and inattention (LB-PA: 1.59 points, P < .001; UF-PA: 3.91 points, P < .001). Conclusions: This research provides promising evidence for the efficacy of LB-PA as a strategy to improve classroom behavior in preschoolers.
A Combination of Recombinant Mycobacterium bovis BCG Strains Expressing Pneumococcal Proteins Induces Cellular and Humoral Immune Responses and Protects against Pneumococcal Colonization and Sepsis
Authors: Goulart Cibelly , Rodriguez Dunia , Kanno Alex I. , Converso Thiago Rojas , Lu Ying-Jie , Malley Richard , Leite Luciana C. C. 
Published: August 2017

ABSTRACT

Pneumococcal diseases remain a substantial cause of mortality in young children in developing countries. The development of potentially serotype-transcending vaccines has been extensively studied; ideally, such a vaccine should include antigens that are able to induce protection against colonization (likely mediated by interleukin-17A [IL-17A]) and invasive disease (likely mediated by antibody). The use of strong adjuvants or alternative delivery systems that are able to improve the immunological response of recombinant proteins has been proposed but poses potential safety and practical concerns in children. We have previously constructed a recombinant
Mycobacterium bovis
BCG strain expressing a pneumococcal surface protein A (PspA)-PdT fusion protein (rBCG PspA-PdT) that was able to induce an effective immune response and protection against sepsis in a prime-boost strategy. Here, we constructed two new rBCG strains expressing the pneumococcal proteins SP 0148 and SP 2108, which confer IL-17A-dependent protection against pneumococcal colonization in mouse models. Immunization of mice with rBCG 0148 or rBCG 2108 in a prime-boost strategy induced IL-17A and gamma interferon (IFN-γ) production. The combination of these rBCG strains with rBCG PspA-PdT (rBCG Mix), followed by a booster dose of the combined recombinant proteins (rMix) induced an IL-17A response against SP 0148 and SP 2108 and a humoral response characterized by increased levels of IgG2c against PspA and functional antibodies against pneumolysin. Furthermore, immunization with the rBCG Mix prime/rMix booster (rBCG Mix/rMix) provides protection against pneumococcal colonization and sepsis. These results suggest the use of combined rBCG strains as a potentially serotype-transcending pneumococcal vaccine in a prime-boost strategy, which could provide protection against pneumococcal colonization and sepsis.
HRAS
,
EGFR
,
MET
, and
RON
Genes Are Recurrently Activated by Provirus Insertion in Liver Tumors Induced by the Retrovirus Myeloblastosis-Associated Virus 2
Authors: Pecenka Vladimir , Pajer Petr , Karafiat Vít , Kasparova Petra , Dudlova Jana , Dvorak Michal 
Published: August 2017

ABSTRACT

Myeloblastosis-associated virus 2 (MAV-2) is a highly tumorigenic simple avian retrovirus. Chickens infected
in ovo
with MAV-2 develop tumors in the kidneys, lungs, and liver with a short latency, less than 8 weeks. Here we report the results of molecular analyses of MAV-2-induced liver tumors that fall into three classes: hepatic hemangiosarcomas (HHSs), intrahepatic cholangiocarcinomas (ICCs), and hepatocellular carcinomas (HCCs). Comprehensive inverse PCR-based screening of 92 chicken liver tumors revealed that in ca. 86% of these tumors, MAV-2 provirus had integrated into one of four gene loci:
HRAS
,
EGFR
,
MET
, and
RON
. Insertionally mutated genes correlated with tumor type:
HRAS
was hit in HHSs,
MET
in ICCs,
RON
mostly in ICCs, and
EGFR
mostly in HCCs. The provirus insertions led to the overexpression of the affected genes and, in the case of
EGFR
and
RON
, also to the truncation of exons encoding the extracellular ligand-binding domains of these transmembrane receptors. The structures of truncated
EGFR
and
RON
closely mimic the structures of oncogenic variants of these genes frequently found in human tumors (
EGFRvIII
and
sfRON
).

IMPORTANCE
These data describe the mechanisms of oncogenesis induced in chickens by the MAV-2 retrovirus. They also show that molecular processes converting cellular regulatory genes to cancer genes may be remarkably similar in chickens and humans. We suggest that the MAV-2 retrovirus-based model can complement experiments performed using mouse models and provide data that could translate to human medicine.
Human Metapneumovirus M2-2 Protein Acts as a Negative Regulator of Alpha Interferon Production by Plasmacytoid Dendritic Cells
Authors: Kitagawa Yoshinori , Sakai Madoka , Funayama Mariko , Itoh Masae , Gotoh Bin 
Published: August 2017

ABSTRACT

Human metapneumovirus (HMPV) has the ability to inhibit Toll-like receptor 7 (TLR7)- and TLR9-dependent alpha interferon (IFN-α) production by plasmacytoid dendritic cells (pDCs). However, the inhibition mechanism remains largely unknown. To identify viral proteins responsible for this inhibition, we performed a screening of HMPV open reading frames (ORFs) for the ability to block TLR7/9-dependent signaling reconstituted in HEK293T cells by transfection with myeloid differentiation factor 88 (MyD88), tumor necrosis factor receptor-associated factor 6 (TRAF6), IKKα, and IFN regulatory factor 7 (IRF7). This screening demonstrated that the M2-2 protein was the most potent inhibitor of TLR7/9-dependent IFN-α induction. A recombinant HMPV in which the M2-2 ORF was silenced indeed induced greater IFN-α production by human pDCs than wild-type HMPV did. Immunoprecipitation experiments showed direct physical association of the M2-2 protein with the inhibitory domain (ID) of IRF7. As a natural consequence of this, transfection of IRF7 lacking the ID, a constitutively active mutant, resulted in activation of the IFN-α promoter even in the presence of M2-2. Bioluminescence resonance energy transfer assays and split
Renilla
luciferase complementation assays revealed that M2-2 inhibited MyD88/TRAF6/IKKα-induced homodimerization of IRF7. In contrast, expression of the M2-2 protein did not result in inhibition of IPS-1-induced homodimerization and resultant activation of IRF7. This indicates that inhibition of MyD88/TRAF6/IKKα-induced IRF7 homodimerization does not result from a steric effect of M2-2 binding. Instead, it was found that M2-2 inhibited MyD88/TRAF6/IKKα-induced phosphorylation of IRF7 on Ser477. These results suggest that M2-2 blocks TLR7/9-dependent IFN-α induction by preventing IRF7 homodimerization, possibly through its effects on the phosphorylation status of IRF7.

IMPORTANCE
The family
Paramyxoviridae
is divided into two subfamilies, the
Paramyxovirinae
and the
Pneumovirinae
. Members of the subfamily
Paramyxovirinae
have the ability to inhibit TLR7/9-dependent IFN-α production, and the underlying inhibition mechanism has been intensively studied. In contrast, little is known about how members of the subfamily
Pneumovirinae
regulate IFN-α production by pDCs. We identified the M2-2 protein of HMPV, a member of the subfamily
Pneumovirinae
, as a negative regulator of IFN-α production by pDCs and uncovered the underlying mechanism. This study explains in part why the M2-2 knockout recombinant HMPV is attenuated and further suggests that M2-2 is a potential target for HMPV therapy.