bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequences of Three Multidrug-Resistant Clinical Isolates of Streptococcus pneumoniae Serotype 19A with Different Susceptibilities to the Myxobacterial Metabolite Carolacton
Authors: Donner Jannik , Bunk Boyke , Schober Isabel , Spröer Cathrin , Bergmann Simone , Jarek Michael , Overmann Jörg , Wagner-Döbler Irene 
Published: February 2017

ABSTRACT

The full-genome sequences of three drug- and multidrug-resistant
Streptococcus pneumoniae
clinical isolates of serotype 19A were determined by PacBio single-molecule real-time sequencing, in combination with Illumina MiSeq sequencing. A comparison to the genomes of other pneumococci indicates a high nucleotide sequence identity to strains Hungary19A-6 and TCH8431/19A.
Metagenome-Assembled Draft Genome Sequence of a Novel Microbial Stenotrophomonas maltophilia Strain Isolated from
Caenorhabditis
remanei
Tissue
Authors: Fierst Janna L. , Murdock Duncan A. , Thanthiriwatte Chamali , Willis John H. , Phillips Patrick C. 
Published: February 2017

ABSTRACT

Stenotrophomonas maltophilia
is a Gram-negative aerobic bacterium and emerging nosocomial pathogen. Here, we present a draft genome sequence for an
S. maltophilia
strain assembled from a metagenomic DNA extract isolated from a laboratory stock of the nematode worm
Caenorhabditis remanei
.
Draft Genome Sequence of a Vibrio harveyi Strain Associated with Vibriosis in Pacific White Shrimp (
Litopenaeus vannamei
)
Authors: Moreno Emille , Parks Marci , Pinnell Lee J. , Tallman James J. , Turner Jeffrey W. 
Published: February 2017

ABSTRACT

Vibrio harveyi
is a Gram-negative bacterium associated with vibriosis in penaeid shrimp. Here, we report the draft genome sequence of a
V. harveyi
strain isolated from Pacific white shrimp (
Litopenaeus vannamei
) during a vibriosis outbreak. The availability of this genome will aid future studies of vibriosis in shrimp aquaculture.
Genome Sequence of Type Strain
Fonsecaea multimorphosa
CBS 980.96
T
, a Causal Agent of Feline Cerebral Phaeohyphomycosis
Authors: Leao Aniele C. Ribas , Weiss Vinicius Almir , Vicente Vania Aparecida , Costa Flavia , Bombassaro Amanda , Raittz Roberto Tadeu , Steffens Maria Berenice R. , Pedrosa Fabio Oliveira , Gomes Renata R. , Baura Valter , Faoro Helisson , Sfeir Michelle Zibetti Tadra , Balsanelli Eduardo , Moreno Leandro F. , Najafzadeh M. Javad , de Hoog Sybren , Souza Emanuel Maltempi 
Published: February 2017

ABSTRACT

A draft genome sequence of type strain
Fonsecaea multimorphosa
CBS 980.96
T
was obtained. This species was first isolated from a cat with cerebral phaeohyphomycosis in Queensland, Australia.
Do failures in non-technical skills contribute to fatal medical accidents in Japan? A review of the 2010–2013 national accident reports
Authors: Uramatsu Masashi , Fujisawa Yoshikazu , Mizuno Shinya , Souma Takahiro , Komatsubara Akinori , Miki Tamotsu 
Published: February 2017

ObjectivesWe sought to clarify how large a proportion of fatal medical accidents can be considered to be caused by poor non-technical skills, and to support development of a policy to reduce number of such accidents by making recommendations about possible training requirements.DesignSummaries of reports of fatal medical accidents, published by the Japan Medical Safety Research Organization, were reviewed individually. Three experienced clinicians and one patient safety expert conducted the reviews to determine the cause of death. Views of the patient safety expert were given additional weight in the overall determination.SettingA total of 73 summary reports of fatal medical accidents were reviewed. These reports had been submitted by healthcare organisations across Japan to the Japan Medical Safety Research Organization between April 2010 and March 2013.Primary and secondary outcome measuresThe cause of death in fatal medical accidents, categorised into technical skills, non-technical skills and inevitable progress of disease were evaluated. Non-technical skills were further subdivided into situation awareness, decision making, communication, team working, leadership, managing stress and coping with fatigue.ResultsOverall, the cause of death was identified as non-technical skills in 34 cases (46.6%), disease progression in 33 cases (45.2%) and technical skills in two cases (5.5%). In two cases, no consensual determination could be achieved. Further categorisation of cases of non-technical skills were identified as 14 cases (41.2%) of problems with situation awareness, eight (23.5%) with team working and three (8.8%) with decision making. These three subcategories, or combinations of them, were identified as the cause of death in 33 cases (97.1%).ConclusionsPoor non-technical skills were considered to be a significant cause of adverse events in nearly half of the fatal medical accidents examined. Improving non-technical skills may be effective for reducing accidents, and training in particular subcategories of non-technical skills may be especially relevant.
Quantum isometry groups of dual of finitely generated discrete groups and quantum groups
Authors: Goswami Debashish , Mandal Arnab 
Published: February 2017

We study quantum isometry groups, denoted by [Formula: see text], of spectral triples on [Formula: see text] for a finitely generated discrete group [Formula: see text] coming from the word-length metric with respect to a symmetric generating set [Formula: see text]. We first prove a few general results about [Formula: see text] including: • For a group [Formula: see text] with polynomial growth property, the dual of [Formula: see text] has polynomial growth property provided the action of [Formula: see text] on [Formula: see text] has full spectrum. •[Formula: see text] for any discrete abelian group [Formula: see text], where [Formula: see text] is a suitable metric on the dual compact abelian group [Formula: see text]. We then carry out explicit computations of [Formula: see text] for several classes of examples including free and direct product of cyclic groups, Baumslag–Solitar group, Coxeter groups etc. In particular, we have computed quantum isometry groups of all finitely generated abelian groups which do not have factors of the form [Formula: see text] or [Formula: see text] for some [Formula: see text] in the direct product decomposition into cyclic subgroups.
The Histone Methyltransferase DOT1L Promotes Neuroblastoma by Regulating Gene Transcription
Authors: Wong Matthew , Tee Andrew E.L. , Milazzo Giorgio , Bell Jessica L. , Poulos Rebecca C. , Atmadibrata Bernard , Sun Yuting , Jing Duohui , Ho Nicholas , Ling Dora , Liu Pei Yan , Zhang Xu Dong , Hüttelmaier Stefan , Wong Jason W.H. , Wang Jenny , Polly Patsie , Perini Giovanni , Scarlett Christopher J. , Liu Tao 
Published: February 2017

Abstract
Myc oncoproteins exert tumorigenic effects by regulating expression of target oncogenes. Histone H3 lysine 79 (H3K79) methylation at Myc-responsive elements of target gene promoters is a strict prerequisite for Myc-induced transcriptional activation, and DOT1L is the only known histone methyltransferase that catalyzes H3K79 methylation. Here, we show that N-Myc upregulates DOT1L mRNA and protein expression by binding to the DOT1L gene promoter. shRNA-mediated depletion of DOT1L reduced mRNA and protein expression of N-Myc target genes ODC1 and E2F2. DOT1L bound to the Myc Box II domain of N-Myc protein, and knockdown of DOT1L reduced histone H3K79 methylation and N-Myc protein binding at the ODC1 and E2F2 gene promoters and reduced neuroblastoma cell proliferation. Treatment with the small-molecule DOT1L inhibitor SGC0946 reduced H3K79 methylation and proliferation of MYCN gene–amplified neuroblastoma cells. In mice xenografts of neuroblastoma cells stably expressing doxycycline-inducible DOT1L shRNA, ablating DOT1L expression with doxycycline significantly reduced ODC1 and E2F2 expression, reduced tumor progression, and improved overall survival. In addition, high levels of DOT1L gene expression in human neuroblastoma tissues correlated with high levels of MYCN, ODC1, and E2F2 gene expression and independently correlated with poor patient survival. Taken together, our results identify DOT1L as a novel cofactor in N-Myc–mediated transcriptional activation of target genes and neuroblastoma oncogenesis. Furthermore, they characterize DOT1L inhibitors as novel anticancer agents against MYCN-amplified neuroblastoma. Cancer Res; 77(9); 2522–33. ©2017 AACR.
Loss of NDRG2 Expression Confers Oral Squamous Cell Carcinoma with Enhanced Metastatic Potential
Authors: Tamura Tomohiro , Ichikawa Tomonaga , Nakahata Shingo , Kondo Yudai , Tagawa Yuri , Yamamoto Koji , Nagai Kentaro , Baba Takashi , Yamaguchi Ryoji , Futakuchi Mitsuru , Yamashita Yoshihiro , Morishita Kazuhiro 
Published: February 2017

Abstract
Loss of the tumor suppressor NDRG2 has been implicated in the development of oral squamous cell carcinoma (OSCC), acting by modulating PI3K/AKT-mediated dephosphorylation of PTEN at S380/S382/T383 (STT). Here, we show that the majority of OSCC tumors with lymph node metastasis, a major prognostic factor, exhibit high levels of phosphorylated AKT-S473 and PTEN-STT and low levels of NDRG2 expression. In Ndrg2-deficient mice, which develop a wide range of tumors, we developed a model of OSCC by treatment with the tobacco surrogate 4-nitroquinoline-1-oxide (4-NQO). In this model, both the number and size of OSCC tumors were increased significantly by Ndrg2 deficiency, which also increased invasion of cervical lymph nodes. 4-NQO treatment of human OSCC cell lines exhibiting low NDRG2 expression induced epithelial–mesenchymal transition via activation of NF-κB signaling. Conversely, ectopic expression of NDRG2 reversed the EMT phenotype and inhibited NF-κB signaling via suppression of PTEN-STT and AKT-S473 phosphorylation. Our results show how NDRG2 expression serves as a critical determinant of the invasive and metastatic capacity of OSCC. Cancer Res; 77(9); 2363–74. ©2017 AACR.
OCTN1 Is a High-Affinity Carrier of Nucleoside Analogues
Authors: Drenberg Christina D. , Gibson Alice A. , Pounds Stanley B. , Shi Lei , Rhinehart Dena P. , Li Lie , Hu Shuiying , Du Guoqing , Nies Anne T. , Schwab Matthias , Pabla Navjotsingh , Blum William , Gruber Tanja A. , Baker Sharyn D. , Sparreboom Alex 
Published: February 2017

Abstract
Resistance to xenobiotic nucleosides used to treat acute myeloid leukemia (AML) and other cancers remains a major obstacle to clinical management. One process suggested to participate in resistance is reduced uptake into tumor cells via nucleoside transporters, although precise mechanisms are not understood. Through transcriptomic profiling, we determined that low expression of the ergothioneine transporter OCTN1 (SLC22A4; ETT) strongly predicts poor event-free survival and overall survival in multiple cohorts of AML patients receiving treatment with the cytidine nucleoside analogue cytarabine. Cell biological studies confirmed OCTN1-mediated transport of cytarabine and various structurally related cytidine analogues, such as 2′deoxycytidine and gemcitabine, occurs through a saturable process that is highly sensitive to inhibition by the classic nucleoside transporter inhibitors dipyridamole and nitrobenzylmercaptopurine ribonucleoside. Our findings have immediate clinical implications given the potential of the identified transport system to help refine strategies that could improve patient survival across multiple cancer types where nucleoside analogues are used in cancer treatment. Cancer Res; 77(8); 2102–11. ©2017 AACR.
Targeted Degradation of BET Proteins in Triple-Negative Breast Cancer
Authors: Bai Longchuan , Zhou Bing , Yang Chao-Yie , Ji Jiao , McEachern Donna , Przybranowski Sally , Jiang Hui , Hu Jiantao , Xu Fuming , Zhao Yujun , Liu Liu , Fernandez-Salas Ester , Xu Jing , Dou Yali , Wen Bo , Sun Duxin , Meagher Jennifer , Stuckey Jeanne , Hayes Daniel F. , Li Shunqiang , Ellis Matthew J. , Wang Shaomeng 
Published: February 2017

Abstract
Triple-negative breast cancers (TNBC) remain clinically challenging with a lack of options for targeted therapy. In this study, we report the development of a second-generation BET protein degrader, BETd-246, which exhibits superior selectivity, potency, and antitumor activity. In human TNBC cells, BETd-246 induced degradation of BET proteins at low nanomolar concentrations within 1 hour of exposure, resulting in robust growth inhibition and apoptosis. BETd-246 was more potent and effective in TNBC cells than its parental BET inhibitor compound BETi-211. RNA-seq analysis revealed predominant downregulation of a large number of genes involved in proliferation and apoptosis in cells treated with BETd-246, as compared with BETi-211 treatment that upregulated and downregulated a similar number of genes. Functional investigations identified the MCL1 gene as a critical downstream effector for BET degraders, which synergized with small-molecule inhibitors of BCL-xL in triggering apoptosis. In multiple murine xenograft models of human breast cancer, BETd-246 and a further optimized analogue BETd-260 effectively depleted BET proteins in tumors and exhibited strong antitumor activities at well-tolerated dosing schedules. Overall, our findings show that targeting BET proteins for degradation represents an effective therapeutic strategy for TNBC treatment. Cancer Res; 77(9); 2476–87. ©2017 AACR.
CDK4/6 Therapeutic Intervention and Viable Alternative to Taxanes in CRPC
Authors: Stice James P. , Wardell Suzanne E. , Norris John D. , Yllanes Alexander P. , Alley Holly M. , Haney Victoria O. , White Hannah S. , Safi Rachid , Winter Peter S. , Cocce Kimberly J. , Kishton Rigel J. , Lawrence Scott A. , Strum Jay C. , McDonnell Donald P. 
Published: February 2017

Abstract
Resistance to second-generation androgen receptor (AR) antagonists and CYP17 inhibitors in patients with castration-resistant prostate cancer (CRPC) develops rapidly through reactivation of the androgen signaling axis and has been attributed to AR overexpression, production of constitutively active AR splice variants, or the selection for AR mutants with altered ligand-binding specificity. It has been established that androgens induce cell-cycle progression, in part, through upregulation of cyclin D1 (CCND1) expression and subsequent activation of cyclin-dependent kinases 4 and 6 (CDK4/6). Thus, the efficacy of the newly described CDK4/6 inhibitors (G1T28 and G1T38), docetaxel and enzalutamide, was evaluated as single agents in clinically relevant in vitro and in vivo models of hormone-sensitive and treatment-resistant prostate cancer. CDK4/6 inhibition (CDK4/6i) was as effective as docetaxel in animal models of treatment-resistant CRPC but exhibited significantly less toxicity. The in vivo effects were durable and importantly were observed in prostate cancer cells expressing wild-type AR, AR mutants, and those that have lost AR expression. CDK4/6i was also effective in prostate tumor models expressing the AR-V7 variant or the AR F876L mutation, both of which are associated with treatment resistance. Furthermore, CDK4/6i was effective in prostate cancer models where AR expression was lost. It is concluded that CDK4/6 inhibitors are a viable alternative to taxanes as therapeutic interventions in endocrine therapy–refractory CRPC.
Implications: The preclinical efficacy of CDK4/6 monotherapy observed here suggests the need for near-term clinical studies of these agents in advanced prostate cancer. Mol Cancer Res; 15(6); 660–9. ©2017 AACR.
Kcnj11
Ablation Is Associated With Increased Nitro-Oxidative Stress During Ischemia-Reperfusion Injury
Authors: Zhang Bo , Novitskaya Tatiana , Wheeler Debra G. , Xu Zhaobin , Chepurko Elena , Huttinger Ryan , He Heng , Varadharaj Saradhadevi , Zweier Jay L. , Song Yanna , Xu Meng , Harrell Frank E. , Su Yan Ru , Absi Tarek , Kohr Mark J. , Ziolo Mark T. , Roden Dan M. , Shaffer Christian M. , Galindo Cristi L. , Wells Quinn S. , Gumina Richard J. 
Published: February 2017

Background—
Despite increased secondary cardiovascular events in patients with ischemic cardiomyopathy (ICM), the expression of innate cardiac protective molecules in the hearts of patients with ICM is incompletely characterized. Therefore, we used a nonbiased RNAseq approach to determine whether differences in cardiac protective molecules occur with ICM.

Methods and Results—

RNAseq analysis of human control and ICM left ventricular samples demonstrated a significant decrease in
KCNJ11
expression with ICM.
KCNJ11
encodes the Kir6.2 subunit of the cardioprotective K
ATP
channel. Using wild-type mice and
kcnj11
-deficient (
kcnj11
-null) mice, we examined the effect of
kcnj11
expression on cardiac function during ischemia-reperfusion injury. Reactive oxygen species generation increased in
kcnj11
-null hearts above that found in wild-type mice hearts after ischemia-reperfusion injury. Continuous left ventricular pressure measurement during ischemia and reperfusion demonstrated a more compromised diastolic function in
kcnj11
-null compared with wild-type mice during reperfusion. Analysis of key calcium-regulating proteins revealed significant differences in
kcnj11
-null mice. Despite impaired relaxation,
kcnj11
-null hearts increased phospholamban Ser16 phosphorylation, a modification that results in the dissociation of phospholamban from sarcoendoplasmic reticulum Ca
2+
, thereby increasing sarcoendoplasmic reticulum Ca
2+
–mediated calcium reuptake. However,
kcnj11
-null mice also had increased 3-nitrotyrosine modification of the sarcoendoplasmic reticulum Ca
2+
-ATPase, a modification that irreversibly impairs sarcoendoplasmic reticulum Ca
2+
function, thereby contributing to diastolic dysfunction.

Conclusions—

KCNJ11
expression is decreased in human ICM. Lack of
kcnj11
expression increases peroxynitrite-mediated modification of the key calcium-handling protein sarcoendoplasmic reticulum Ca
2+
-ATPase after myocardial ischemia-reperfusion injury, contributing to impaired diastolic function. These data suggest a mechanism for ischemia-induced diastolic dysfunction in patients with ICM.
Understanding the Revival of the Indian Summer Monsoon after Breaks
Authors: Govardhan Dandu , Rao Vadlamudi Brahmananda , Ashok Karumuri 
Published: February 2017

AbstractIn this paper, the authors suggest a dynamical mechanism involved in the revival of the summer monsoon after breaks. In this context, the authors carry out a diagnostic analysis using the datasets from National Centers for Environmental Prediction Reanalysis 2 for the period 1979–2007 to identify a robust mechanism that typifies breaks and subsequent revival of monsoon. The authors find during the peak of significant breaks an anomalous southward shift of the subtropical westerly jet stream, which is invariably accompanied by an anomalous northward shift of a stronger-than-normal easterly jet. These major changes during a break facilitate an instability mechanism, which apparently leads to formation of a synoptic disturbance. Formation of such a disturbance is critical to the subsequent revival of the summer monsoon in 61% of the observed break-to-active revivals.Computations of energetics and correlation analysis carried out suggest an increase in the eddy kinetic energy at the expense of the mean kinetic energy during the breaks, in agreement with the formation of the synoptic disturbance. This demonstrates that barotropic instability in the presence of a monsoon basic flow is the primary physical mechanism that controls the revival of the summer monsoon subsequent to the break events.
Nonlinear ENSO Warming Suppression (NEWS)
Authors: Kohyama Tsubasa , Hartmann Dennis L. 
Published: February 2017

Abstract
In global warming experiments, the majority of global climate models warm faster in the eastern equatorial Pacific than in the west and produce a weakening of the Walker circulation. In contrast, GFDL-ESM2M is an exception that exhibits a La Niña–like mean-state warming with a strengthening of the Walker circulation. This study explores the cause of this exceptional response and proposes a new mechanism, the nonlinear ENSO warming suppression (NEWS), where the transient heating rate difference between the atmospheric and oceanic reservoirs annihilates extreme El Niños, causing a suppression of mean-state warming in the east. Heat budget analyses of GFDL-ESM2M robustly show that nonlinear dynamical heating, which is necessary for extremely warm El Niños, becomes negligible under warming. An idealized nonlinear recharge oscillator model suggests that, if the temperature difference between the atmospheric and oceanic reservoirs becomes larger than some threshold value, the upwelling becomes too efficient for El Niño–Southern Oscillation (ENSO) to retain its nonlinearity. Therefore, extreme El Niños dissipate but La Niñas remain almost unchanged, causing a La Niña–like mean-state warming. NEWS is consistent with observations and GFDL-ESM2M but not with the majority of state-of-the-art models, which lack realistic ENSO nonlinearity. NEWS and its opposite response to atmospheric cooling, the nonlinear ENSO cooling suppression (NECS), might contribute to the Pacific multidecadal natural variability and global warming hiatuses.
Variability of Cloudiness over Mountain Terrain in the Western United States
Authors: Sumargo Edwin , Cayan Daniel R. 
Published: February 2017

Abstract
This study investigates the spatial and temporal variability of cloudiness across mountain zones in the western United States. Daily average cloud albedo is derived from a 19-yr series (1996–2014) of half-hourly Geostationary Operational Environmental Satellite (GOES) images. During springtime when incident radiation is active in driving snowmelt–runoff processes, the magnitude of daily cloud variations can exceed 50% of long-term averages. Even when aggregated over 3-month periods, cloud albedo varies by ±10% of long-term averages in many locations. Rotated empirical orthogonal functions (REOFs) of daily cloud albedo anomalies over high-elevation regions of the western conterminous United States identify distinct regional patterns, wherein the first five REOFs account for ~67% of the total variance. REOF1 is centered over Northern California and Oregon and is pronounced between November and March. REOF2 is centered over the interior northwest and is accentuated between March and July. Each of the REOF/rotated principal components (RPC) modes associates with anomalous large-scale atmospheric circulation patterns and one or more large-scale teleconnection indices (Arctic Oscillation, Niño-3.4, and Pacific–North American), which helps to explain why anomalous cloudiness patterns take on regional spatial scales and contain substantial variability over seasonal time scales.