bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progressing “Positive Epidemiology”: A Cross-national Analysis of Adolescents’ Positive Mental Health and Outcomes During the COVID-19 Pandemic
Authors: O’Connor Meredith , Olsson Craig A. , Lange Katherine , Downes Marnie , Moreno-Betancur Margarita , Mundy Lisa , Viner Russell M. , Goldfeld Sharon , Patton George , Sawyer Susan M. , Hope Steven 
Published: October 2024

Purpose:
“Positive epidemiology” emphasizes strengths and assets that protect the health of populations. Positive mental health refers to a range of social and emotional capabilities that may support adaptation to challenging circumstances. We examine the role of positive mental health in promoting adolescent health during the crisis phase of the COVID-19 pandemic.

Methods:
We used four long-running Australian and UK longitudinal cohorts: Childhood to Adolescence Transition Study (CATS; analyzed N = 809; Australia); Longitudinal Study of Australian Children (LSAC) – Baby (analyzed N =1,534) and Kindergarten (analyzed N = 1,300) cohorts; Millennium Cohort Study (MCS; analyzed N = 2,490; United Kingdom). Measures included prepandemic exposure: positive mental health (parent reported, 13–15 years) including regulating emotions, interacting well with peers, and caring for others; and pandemic outcomes: psychological distress, life satisfaction, and sleep and alcohol use outside of recommendations (16–21 years; 2020). We used a two-stage meta-analysis to estimate associations between positive mental health and outcomes across cohorts, accounting for potential confounders.

Results:
Estimates suggest meaningful effects of positive mental health on psychosocial outcomes during the pandemic, including lower risk of psychological distress (risk ratio [RR] = 0.83, 95% confidence interval [CI] = 0.71, 0.97) and higher life satisfaction (RR = 1.1, 95% CI = 1.0, 1.2). The estimated effects for health behaviors were smaller in magnitude (sleep: RR = 0.95, 95% CI = 0.86, 1.1; alcohol use: RR = 0.97, 95% CI = 0.85, 1.1).

Conclusions:
Our results are consistent with the hypothesis that adolescents’ positive mental health supports better psychosocial outcomes during challenges such as the COVID-19 pandemic, but the relevance for health behaviors is less clear. These findings reinforce the value of extending evidence to include positive health states and assets.
Deubiquitinase USP47 Ameliorates Cardiac Hypertrophy Through Reducing Protein O-GlcNAcylation
Authors: Jiang Yu , Cai Wenyao , Lei Guangtao , Cai Guorong , Wu Qinghua , Lu Peng 
Published: October 2024

Abstract:
Cardiac hypertrophy is a crucial risk factor for heart failure when the heart is confronted with physiologic or pathologic stimuli. The ubiquitin–proteasome system plays a critical role in the pathogenesis of cardiac hypertrophy. However, as a key component of the ubiquitin–proteasome system, the role of deubiquitinating enzymes in cardiac hypertrophy is not well understood. In this study, we observed that the expression level of deubiquitinase USP47 was increased in hypertrophic hearts and angiotensin II (Ang II)-stimulated neonatal rat cardiomyocytes. Adenovirus-mediated gain- and loss-of-function approaches indicated that USP47 overexpression significantly attenuated Ang II-induced cardiac hypertrophy in vitro and in vivo, whereas endogenous USP47 deficiency promoted the prohypertrophic effect of Ang II. Further investigation demonstrated that USP47 inhibited O-GlcNAcylation in cardiomyocytes by controlling the expression of O-GlcNAcase. Mechanistically, USP47 bound, deubiquitinated, and stabilized protein arginine methyltransferase 5 (PRMT5), thus upregulating O-GlcNAcase expression. We found that the restoration of PRMT5 abolished the prohypertrophic effects of USP47 silence in vitro. Therefore, our results provide the first evidence of the involvement of USP47 in cardiac hypertrophy and identify USP47 as a potential target for hypertrophic therapy.
Liquid Chromatography–Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Dalbavancin in Plasma of Pediatric and Young Adult Patients
Authors: Cafaro Alessia , Mariani Marcello , Pigliasco Federica , Baiardi Giammarco , Barco Sebastiano , Biondi Margherita , Mesini Alessio , Russo Chiara , Saffioti Carolina , Mattioli Francesca , Castagnola Elio , Cangemi Giuliana 
Published: October 2024

Background:
Dalbavancin, an antimicrobial lipoglycopeptide, is authorized in Europe for treating acute bacterial infections of the skin and skin structures in adults and pediatric patients aged 3 months and older. However, off-label dosing regimens have been proposed for various indications beyond acute bacterial infections of the skin and skin structures. This study presents a novel bioanalytical method using liquid chromatography–tandem mass spectrometry to quantify dalbavancin in low-volume plasma samples (50 μL).

Methods:
The method underwent validation in accordance with international guidelines for bioanalytical method validation and was applied to 9 clinical samples obtained from pediatric and young adult patients undergoing dalbavancin therapy. Liquid chromatography–tandem mass spectrometry analyses were conducted at the G. Gaslini Institute in Genoa, Italy, utilizing an Ultimate 3000 ultra high performance liquid chromatography system coupled to a TSQ Quantiva Triple Quadrupole system (Thermo Fisher Scientific, Milan, Italy). The analytical procedure involved the addition of deuterated dalbavancin as internal standard and a rapid extraction from 50 µL of human plasma, followed by chromatographic separation on a Thermo Scientific Accucore Polar Premium column. Accurate quantification of the analyte was achieved through multiple reaction monitoring detection.

Results:
The assay exhibited linearity within the concentration range of 0.66–400 mcg/mL in plasma, demonstrating accuracy and reproducibility in the absence of matrix effects. Stability testing was conducted on both quality controls and real samples to establish a robust protocol under real-life conditions.

Conclusions:
This fast and reliable dalbavancin quantitation method could improve current pediatric clinical practice by enabling data collection for future dose recommendations in special patient populations.
Pre-sacral glomangioma: a rare localization of glomus tumors: case report
Authors: Mseddi Mohamed Ali , Siala Rakia , Yaakoubi Chaima , Saad Sarra , Zeheni Kassar Alia , Nouri Takwa , Guizeni Rami , Sassi Karim , Ben Slima Mohamed 
Published: October 2024

Introduction and importance:
Deep-located glomangiomas are rarely reported. Because of their scarcity, treatment strategy is hard to establish. Herein, the authors report the first case to our knowledge of pre-sacral glomangioma.

Case presentation:
A 34-year-old female patient, with no previous medical history, consulted for 2-month-old pelvic abdominal pain, vomiting and delayed menstruation. Her physical and biological parameters were with no abnormalities. MRI of the pelvis demonstrated a 14 cm mixed heterogeneous pre-sacral lesion pushing the rectum anteriorly. She was operated on via a laparoscopic approach. Division of Douglas’ pouch and pelvic peritoneum laterally to the bladder showcased a cystic lesion of 13×8 cm occupying the pelvis while deviating the rectum anteriorly. Its content was aspirated and left membrane was extracted in a sac. The postoperative course was uneventful.

Discussion:
Pre-sacral masses are hard to treat because of their large heterogeneity. Surgical resection should be tempted to retrieve the definitive histological diagnosis and relieve the patient. However, the surgical route is controversial as each approach has its advantages. Thus, the surgical route should take into consideration the lesion’s size, height and surrounding contacts, the patient’s functional state and surgeon’s expertise.

Conclusion:
Pre-sacral glomangiomas carries a low malignant pattern but should be resected to offer histological diagnosis. The surgical route remains at the surgeon’s decision, with the main objective to totally resect the encountered lesion without causing functional and sexual complications or harm to surrounding viscera.
Hodgkin lymphoma in pre-existing thymoma: a case report
Authors: AbuKhalaf Rahaf M. , Zeer Zahraa M. M. , Abu Harb Zahra Hosam , Kamal Nazmi , Alseedat Yasmin , Sughayer Maher A. 
Published: October 2024

Introduction:
Thymus is a primary lymphoid organ which has an important role in humoral and cellular immunity. It can be a site for various neoplasms including thymomas.

Case presentation:
The authors report a case of a 55-year-old patient presenting with weight loss, night sweats and sensation of heat. After thorough evaluation his histopathology analysis revealed the rare presence of Hodgkin lymphoma in a background of type B2 thymoma.

Discussion:
Thymoma is one of the well-known thymic tumors that is encountered in clinical practice. It has diverse associations with other autoimmune diseases and malignancies. The concurrent diagnosis of thymoma with thymic Hodgkin lymphoma is extremely rare.

Conclusion:
Although this association is very rare, it is crucial to distinguish between thymic Hodgkin lymphoma and thymoma and manage them appropriately.
Identification of potential immune-related genes and infiltrations in temporomandibular joint osteoarthritis
Authors: Zhu Mengjiao , Xing Min , Sun Ruinan , Li Minhui , Qian Wenhao , Fan Mingyue 
Published: October 2024

Objective:
The aim of this study was to investigate the potential inflammatory cytokines and chemokines markers for temporomandibular joint osteoarthritis (TMJOA) diagnosis using a bioinformatics analysis.

Methods:
The differentially expressed genes of mRNA (DEGs) and transcripts of lncRNA (DETs) were identified between TMJOA samples and normal controls curated from GSE205389 by the “DESeq. 2” R package. KEGG and GO were conducted using the R package “ggplot2” and “clusterProfiler”. A PPI network was constructed to identify hub genes by using the STRING and Cytoscape. The co-expression network was constructed between mRNA and lncRNA to check the potential regulation and function of lncRNA on protein-coding genes. Finally, the immune cell infiltration analysis was conducted with CIBERSORTx and confirmed with xCells.

Results:
The authors identified 171 DEGs and DETs, of which the DEGs were closely related to immune response, T-cell activation, cytokine-cytokine-receptor interaction, and the muscle system process. PPI network of the DEGs screened the top 10 hub genes, including IL6, IL1B, IL10, CCL2, CCL5, CXCL1, CXCL10, ICAM1, CSF1 and MMP1. Additionally, the immune cell infiltration analysis showed that CD8+ T cells, M1 macrophage and B cells infiltration were increased in TMJOA samples. Finally, the authors demonstrated that the co-expression between mRNA and lncRNA was mainly enriched in inflammatory and muscle-related pathways.

Conclusions:
The authors found that immune and muscle system-related pathways as well as the immune infiltration played a significant role in the TMJOA development. Additionally, inflammatory cytokines and chemokines could be crucial markers for early-stage TMJOA diagnosis and personalized treatment strategies.
Significance of Image Reconstruction Parameters for Future Lung Cancer Risk Prediction Using Low-Dose Chest Computed Tomography and the Open-Access Sybil Algorithm
Authors: Simon Judit , Mikhael Peter , Graur Alexander , Chang Allison E.B. , Skates Steven J. , Osarogiagbon Raymond U. , Sequist Lecia V. , Fintelmann Florian J. 
Published: October 2024

Purpose
Sybil is a validated publicly available deep learning–based algorithm that can accurately predict lung cancer risk from a single low-dose computed tomography (LDCT) scan. We aimed to study the effect of image reconstruction parameters and CT scanner manufacturer on Sybil's performance.

Materials and Methods
Using LDCTs of a subset of the National Lung Screening Trial participants, which we previously used for internal validation of the Sybil algorithm (test set), we ran the Sybil algorithm on LDCT series pairs matched on kilovoltage peak, milliampere-seconds, reconstruction interval, reconstruction diameter, and either reconstruction filter or axial slice thickness. We also evaluated the cumulative effect of these parameters by combining the best- and the worst-performing parameters. A subanalysis compared Sybil's performance by CT manufacturer. We considered any LDCT positive if future lung cancer was subsequently confirmed by biopsy or surgical resection. The areas under the curve (AUCs) for each series pair were compared using DeLong's test.

Results
There was no difference in Sybil's performance between 1049 pairs of standard versus bone reconstruction filter (AUC at 1 year 0.84 [95% confidence interval (CI): 0.70–0.99] vs 0.86 [95% CI: 0.75–0.98], P = 0.87) and 1961 pairs of standard versus lung reconstruction filter (AUC at 1 year 0.98 [95% CI: 0.97–0.99] vs 0.98 [95% CI: 0.96–0.99], P = 0.81). Similarly, there was no difference in 1288 pairs comparing 2-mm versus 5-mm axial slice thickness (AUC at 1 year 0.98 [95% CI: 0.94–1.00] vs 0.99 [95% CI: 0.97–0.99], P = 0.68). The best-case scenario combining a lung reconstruction filter with 2-mm slice thickness compared with the worst-case scenario combining a bone reconstruction filter with 2.5-mm slice thickness uncovered a significantly different performance at years 2–4 (P = 0.03). Subanalysis showed no significant difference in performance between Siemens and Toshiba scanners.

Conclusions
Sybil's predictive performance for future lung cancer risk is robust across different reconstruction filters and axial slice thicknesses, demonstrating its versatility in various imaging settings. Combining favorable reconstruction parameters can significantly enhance predictive ability at years 2–4. The absence of significant differences between Siemens and Toshiba scanners further supports Sybil's versatility.
Prospective Multicenter Comprehensive Survey on Male Sexual Dysfunction following Laparoscopic, Robotic, and Transanal Approaches for Rectal Cancer (the LANDMARC Study)
Authors: Numata Masakatsu , Yamaguchi Tomohiro , Shiomi Akio , Inada Ryo , Shiozawa Manabu , Kazama Keisuke , Hotchi Masanori , Yamamoto Daisuke , Hasegawa Suguru , Miguchi Masashi , Ohnuma Shinobu , Uehara Kay , Munakata Koji , Kinugasa Yusuke , Horie Hisanaga , Yamaguchi Shigeki , Takeshima Teppei , Hida Koya , Akagi Tomonori , Kagawa Hiroyasu , Oyamada Shunsuke , Rino Yasushi , Sakai Yoshiharu , Watanabe Masahiko , Naitoh Takeshi ,   LANDMARC Collaborative Study Group of the Japan Society of Laparoscopic Colorectal Surgery
Published: October 2024

Objective:
To investigate the incidence of male sexual dysfunction (SD) including erectile dysfunction (ErD) and ejaculatory dysfunction (EjD) after minimally invasive rectal cancer surgery.

Background:
Male SD significantly affects post-rectal cancer surgery quality of life (QOL). Current assessments using the International Index of Erectile Function-5 are unsuitable for patients with reduced postoperative sexual activity, because it assumes sexual intercourse. This study addresses this gap using the Erection Hardness Score (EHS) and custom ejaculatory questionnaires.

Methods:
This prospective multicenter open-label phase II trial enrolled 399 patients who underwent laparoscopic (Lap), robotic (Ro), or transanal (Ta) rectal cancer surgery. EHS and custom ejaculatory questionnaires assessed ErD, EjD, and potency impairment at 3, 6, and 12 months postoperatively. The rates were assessed in the full analysis set and compared between the Lap and Ro groups after propensity score matching.

Results:
At 12 months, the overall incidences of ErD and EjD were 34.7% and 29.8%, respectively. The Ro group showed a significantly lower EjD rate (25.0%) than the Lap group (40.9%), with no significant difference in ErD. Potency impairment was lower in the Ro group at 6 months (32.7% vs. 22.3%) and 12 months (29.0% vs. 17.8%) postoperatively. The Ta group showed relatively high ErD and EjD at 3 months, with some recovery at 12 months.

Conclusions:
Minimally invasive rectal cancer surgery commonly results in ErD, EjD, and potency impairment. Robotic surgery provides lower EjD rates and less potency impairment. Comprehensive sexual function assessments are essential to inform patients and improve QOL outcomes.
Single-Trial Characterisation of Frontal Theta and Parietal Alpha Oscillatory Episodes during Spatial Navigation in Humans
Authors: Cuello Mireia Torralba , Marti-Marca Angela , Pápai Márta Szabina , Soto-Faraco Salvador 
Published: October 2024

ABSTRACTTheoretical proposals and empirical findings both highlight the relevance of theta brain oscillations in human Spatial Navigation. However, whilst the general assumption is that the relevant theta band activity is purely oscillatory, most empirical studies fail to disentangle oscillatory episodes from wide band activity. In addition, experimental approaches often rely on averaged activity across trials and subjects, disregarding moment-to-moment fluctuations in Theta activity, contingent on key aspects of the task. Here, we used novel oscillation detection approaches to investigate the dynamics of theta and alpha episodes in human subjects performing a spatial navigation task in a Virtual Reality environment, resolved at single-trial level. The results suggest that bouts of theta oscillatory activity are related to task difficulty and access to previously encoded information, across different timescales. alpha episodes, instead, seem to anticipate successful navigational decisions and could be related to shifts in internal attention.
Mu and Delta Opioid Receptors Modulate Inhibition within the Prefrontal Cortex Through Dissociable Cellular and Molecular Mechanisms
Authors: Cole Rebecca H. , Joffe Max E. 
Published: October 2024

AbstractAberrant signaling within cortical inhibitory microcircuits has been identified as a common signature of neuropsychiatric disorders. Interneuron (IN) activity is precisely regulated by neuromodulatory systems that evoke widespread changes in synaptic transmission and principal cell output. Cortical interneurons express high levels of Mu and Delta opioid receptors (MOR and DOR), positioning opioid signaling as a critical regulator of inhibitory transmission. However, we lack a complete understanding of how MOR and DOR regulate prefrontal cortex (PFC) microcircuitry. Here, we combine whole-cell patch-clamp electrophysiology, optogenetics, and viral tools to provide an extensive characterization MOR and DOR regulation of inhibitory transmission. We show that DOR activation is more effective at suppressing spontaneous inhibitory transmission in the prelimbic PFC, while MOR causes a greater acute suppression of electrically-evoked GABA release. Cell type-specific optogenetics revealed that MOR and DOR differentially regulate inhibitory transmission from parvalbumin, somatostatin, cholecystokinin, and vasoactive intestinal peptide-expressing INs. Finally, we demonstrate that DOR regulates inhibitory transmission through pre- and postsynaptic modifications to IN physiology, whereas MOR function is predominantly observed in somato-dendritic or presynaptic compartments depending on cell type.Significance StatementThe endogenous opioid system regulates behaviors that rely on prefrontal cortex (PFC) function. Previous studies have described Mu and Delta opioid receptor expression within cortical GABAergic interneurons, but a detailed understanding of how opioids regulate different interneuron subtypes and cortical microcircuits has not been reported. We use whole-cell patch-clamp electrophysiology, genetically engineered mice, and optogenetics to assess MOR and DOR regulation of PFC inhibitory transmission, demonstrating that MOR and DOR inhibition of interneurons display qualitative and quantitative variation across GABAergic circuits within mouse prelimbic PFC.
Cancer-associated adipocytes mediate CD8+T cell dysfunction via FGF21-driven lipolysis
Authors: Dalangood Sumiya , Hu Cegui , Yuan Chenwei , Li Xiang , Qiao Wen , Li Hanjun , Zhang Rongyu , Li Luying , Li Peng , Yu Xiang , Yin Wenjin , Lu Jinsong , Gui Jun 
Published: October 2024

AbstractCancer-associated adipocytes (CAAs) reprogram metabolic status of tumor microenvironment (TME). The metabolic crosstalk between CAAs and CD8+T cells in TME remains unclear. Here we report that CAAs undergo lipolysis, releasing free fatty acids that promote lipid peroxidation and disturb mitochondrial homeostasis in CD8+T cells, leading to their functional exhaustion. Importantly, we uncover that fibroblast growth factor 21 (FGF21) autocrinally drives CAA lipolysis through upregulating adipose triglyceride lipase (ATGL) via FGFR1/KLB-p38 signaling. FGF21 deletion in adipose tissue or ATGL inhibition impedes CAA lipolysis, mitigates lipid peroxidation, normalizes mitochondrial dynamics of CD8+T cells, and restores their effector function, consequently blunting tumor growth. Moreover, combining ATGL inhibitor with anti-PD-1 therapy synergistically enhances the antitumor activity of CD8+T cells, yielding greater therapeutic efficacy. Our findings highlight the pivotal role of CAA lipol ysis in CD8+T cell dysfunction within TME, suggesting that targeting CAA lipolysis represents a valuable avenue for improving cancer immunotherapy.
Basal EFNA3 Facilitates Luminal-Driving Mammary Epithelial Migration and Cancer Metastasis by Promoting OXPHOS
Authors: Chen Jing , ma Rongze , Feng Deyi , Deng Zhixuan , Lu Yunzhe , Xia Kun , Klein Ophir D. , Lu Pengfei 
Published: October 2024

ABSTRACTCollective migration is essential for tissue development, repair, and cancer metastasis. However, the mechanisms by which different cell types coordinate to facilitate cohesive movement remain poorly understood. Here, we report distinct migratory behaviors in luminal and basal cells during collective migration of the mammary epithelium. We show that luminal cells are leaders of directional migration. However, basal cells also play a central role in promoting collective migration by producing EFNA3, which targets luminal cells and enhances oxidative phosphorylation and energy production. This promotion of epithelial migration and branching morphogenesis is confirmed in vivo. Importantly, EFNA3 is a prognostic marker for breast cancer metastasis, with high expression correlating with increased metastasis. Conversely, inhibition of EFNA3 significantly slows metastasis. These findings reveal a novel mechanism of epithelial migration that is critical for organogenesis and identify EFNA3 as a potential therapeutic target for cancer metastasis.
p-ClustVal: A Novelp-adic Approach for Enhanced Clustering of High-Dimensional Single Cell RNASeq Data
Authors: Sharma Parichit , Mishra Sarthak , Kurban Hasan , Dalkilic Mehmet 
Published: October 2024

AbstractThis paper introducesp-ClustVal, a novel data transformation technique inspired byp-adic number theory that significantly enhances cluster discernibility in genomics data, specifically Single Cell RNA Sequencing (scRNASeq). By leveragingp-adic-valuation,p-ClustVal integrates with and augments widely used clustering algorithms and dimension reduction techniques, amplifying their effectiveness in discovering meaningful structure from data. The transformation uses a data-centric heuristic to determine optimal parameters, without relying on ground truth labels, making it more user-friendly.p-ClustVal reduces overlap between clusters by employing alternate metric spaces inspired byp-adic-valuation, a significant shift from conventional methods. Our comprehensive evaluation spanning 30 experiments and over 1400 observations, shows thatp-ClustVal improves performance in 91% of cases, and boosts the performance of classical and state of the art (SOTA) methods. This work contributes to data analytics and genomics by introducing a unique data transformation approach, enhancing downstream clustering algorithms, and providing empirical evidence ofp-ClustVal’s efficacy. The study concludes with insights into the limitations ofp-ClustVal and future research directions.