bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generalized rapid-distortion theory on transversely sheared mean flows with physically realizable upstream boundary conditions: application to trailing-edge problem
Authors: Goldstein M. E. , Leib S. J. , Afsar M. Z. 
Published: July 2017

This paper is concerned with rapid-distortion theory on transversely sheared mean flows which (among other things) can be used to analyse the unsteady motion resulting from the interaction of a turbulent shear flow with a solid surface. It extends previous analyses of Goldsteinet al. (J. Fluid Mech., vol. 736, 2013a, pp. 532–569;NASA/TM-2013-217862, 2013b) which showed that the unsteady motion is completely determined by specifying two arbitrary convected quantities. The present paper uses a pair of previously derived conservation laws to derive upstream boundary conditions that relate these quantities to experimentally measurable flow variables. The result is dependent on the imposition of causality on an intermediate variable that appears in the conservation laws. Goldsteinet al.(2013a) related the convected quantities to the physical flow variables at the location of the interaction, but the results were not generic and hard to reconcile with experiment. That problem does not occur in the present formulation, which leads to a much simpler and more natural result than the one given in Goldsteinet al.(2013a). We also show that the present formalism yields better predictions of the sound radiation produced by the interaction of a two-dimensional jet with the downstream edge of a flat plate than the Goldsteinet al.(2013a) result. The role of causality is also discussed.
Interaction of instability waves and a three-dimensional roughness element in a boundary layer
Authors: de Paula I. B. , Würz W. , Mendonça M. T. , Medeiros M. A. F. 
Published: July 2017

The influence of a single roughness element on the evolution of two-dimensional (2-D) Tollmien–Schlichting (TS) waves is investigated experimentally. Experiments are carried out in a region of zero pressure gradient of an airfoil section. Downstream from the disturbance source, TS waves interact with a cylindrical roughness element with a slowly oscillating height. The oscillation frequency of the roughness was approximately 1500 times lower than the wave frequency and approximately 250 times slower than the characteristic time of flow passing the region of transition development. Therefore, the roughness behaved as a quasi-steady disturbance. The set-up enabled us to perform hot-wire measurements phase locked to the waves and to the roughness movement. Experimental results show a scattering of the 2-D waves into oblique ones and a relatively weak distortion of the mean flow for roughness heights as large as 0.2 times the boundary layer displacement thickness ($\unicode[STIX]{x1D6FF}^{\ast }$). Transfer functions for TS wave scattering at the roughness are obtained. Results show an unexpected coincidence in shape with acoustic receptivity functions found in Würz et al. (J. Fluid Mech., vol. 478, 2003, pp. 135–163) for the problem of excitation of TS waves by scattering of acoustic waves at surface roughness. In the present work, the ratio between the incoming 2-D wave amplitude to the amplitude of the scattered oblique waves scaled linearly with the roughness height only for very shallow roughness. For roughness elements higher than $0.08\unicode[STIX]{x1D6FF}^{\ast }$ and below $0.2\unicode[STIX]{x1D6FF}^{\ast }$, the wave scattering exhibited a quadratic variation with respect to the roughness height. In addition, this feature did not vary significantly with respect to TS wave frequency. An analysis of the weakly nonlinear interactions triggered by the roughness element is also carried out, assisted by numerical solution of nonlinear parabolized stability equations, performed for a two-dimensional Blasius boundary layer. A comparison between experiments and simulations reveals that the weakly nonlinear interactions observed are not substantially affected by mean flow distortions that could be produced in the wake of the small and medium sized roughness elements ($h<0.2\unicode[STIX]{x1D6FF}^{\ast }$). From a practical perspective, results suggest that scattering coefficients might be employed to include the effect of isolated and medium sized roughness elements in transition prediction tools developed for smooth surfaces.
Pore-filling events in single junction micro-models with corresponding lattice Boltzmann simulations
Authors: Zacharoudiou Ioannis , Chapman Emily M. , Boek Edo S. , Crawshaw John P. 
Published: July 2017

The aim of this work is to better understand fluid displacement mechanisms at the pore scale in relation to capillary-filling rules. Using specifically designed micro-models we investigate the role of pore body shape on fluid displacement during drainage and imbibition via quasi-static and spontaneous experiments at ambient conditions. The experimental results are directly compared to lattice Boltzmann (LB) simulations. The critical pore-filling pressures for the quasi-static experiments agree well with those predicted by the Young–Laplace equation and follow the expected filling events. However, the spontaneous imbibition experimental results differ from those predicted by the Young–Laplace equation; instead of entering the narrowest available downstream throat the wetting phase enters an adjacent throat first. Thus, pore geometry plays a vital role as it becomes the main deciding factor in the displacement pathways. Current pore network models used to predict displacement at the field scale may need to be revised as they currently use the filling rules proposed by Lenormandet al.(J. Fluid Mech., vol. 135, 1983, pp. 337–353). Energy balance arguments are particularly insightful in understanding the aspects affecting capillary-filling rules. Moreover, simulation results on spontaneous imbibition, in excellent agreement with theoretical predictions, reveal that the capillary number itself is not sufficient to characterise the two phase flow. The Ohnesorge number, which gives the relative importance of viscous forces over inertial and capillary forces, is required to fully describe the fluid flow, along with the viscosity ratio.
Afterword: Genre Queries, African Studies
Authors: Adejunmobi Moradewun 
Published: July 2017

AbstractThis essay on genres acknowledges the constructed nature of the fluid classifications that form around recognized and recognizable types of creative work. Some systems of classification owe their recognition to powerful cultural intermediaries. Others, often located in the realm of popular culture, emerge without input from cultural intermediaries. As constructed groupings of works, genres matter for their ability to generate distinct circuits of transmission that allow for a specific type of encounter with the work and a type of response to the work.
New parasitological findings for pre-Hispanic camelids
Authors: TAGLIORETTI V. , FUGASSA M. H. , RINDEL D. , SARDELLA N. H. 
Published: July 2017

SUMMARYPaleoparasitological examination provides information of parasite–host associations in the past, shedding light on the geographical origin of some parasites, on the possible dispersal routes and on some of the processes that modelled the parasitic communities. The aim of the present study was to examine parasite remains present in camelid coprolites collected from the archaeological site Alero Destacamento Guardaparque, Patagonia and to discuss the paleoparasitological findings in a biogeographical and paleoecological context. Coprolites were collected from different stratified layers dating from middle to late Holocene, a period covering approximately 7000 years. Paleoparasitological examination revealed the presence of eggs attributed to Lamanema chavezi or Nematodirus lamae, Nematodirus spathiger, Dictyocaulus sp., eggs of two unidentified capillariids, Strongylus-type eggs and oocysts of Eimeria macusaniensis. Enteric parasites of camelids had not changed significantly during the Holocene up to the entry of introduced livestock, although environmental conditions fluctuated greatly throughout this period, indicating the stability of these associations over time. This is the first finding of N. spathiger and Dictyocaulus sp. in paleoparasitological record and their presence are associated with the interaction of camelids with introduced livestock, which likely allowed parasite host switching. In the present study, the zoonotic importance of parasites of camelids is also discussed.
The difference between ice cream and Nazis: Moral externalization and the evolution of human cooperation
Authors: Stanford P. Kyle 
Published: July 2017

AbstractA range of empirical findings is first used to more precisely characterize our distinctive tendency to objectify or externalize moral demands and obligations, and it is then argued that this salient feature of our moral cognition represents a profound puzzle for evolutionary approaches to human moral psychology that existing proposals do not help resolve. It is then proposed that such externalization facilitated a broader shift to a vastly more cooperative form of social life by establishing and maintaining a connection between the extent to which an agent is herself motivated by a given moral norm and the extent to which she uses conformity to that same norm as a criterion in evaluating candidate partners in social interaction generally. This connection ensures the correlated interaction necessary to protect those prepared to adopt increasingly cooperative, altruistic, and other prosocial norms of interaction from exploitation, especially as such norms were applied in novel ways and/or to novel circumstances and as the rapid establishment of new norms allowed us to reap still greater rewards from hypercooperation. A wide range of empirical findings is then used to support this hypothesis, showing why the status we ascribe to moral demands and considerations exhibits the otherwise puzzling combination of objective and subjective elements that it does, as well as showing how the need to effectively advertise our externalization of particular moral commitments generates features of our social interaction so familiar that they rarely strike us as standing in need of any explanation in the first place.
Platinum pyrithione induces apoptosis in chronic myeloid leukemia cells resistant to imatinib via DUB inhibition-dependent caspase activation and Bcr-Abl downregulation
Authors: Lan Xiaoying , Zhao Chong , Chen Xin , Zhang Peiquan , Zang Dan , Wu Jinjie , Chen Jinghong , Long Huidan , Yang Li , Huang Hongbiao , Wang Xuejun , Shi Xianping , Liu Jinbao 
Published: July 2017

AbstractChronic myelogenous leukemia (CML) is characterized by the chimeric tyrosine kinase Bcr-Abl. T315I Bcr-Abl is the most notorious point mutation to elicit acquired resistance to imatinib (IM), leading to poor prognosis. Therefore, it is urgent to search for additional approaches and targeting strategies to overcome IM resistance. We recently reported that platinum pyrithione (PtPT) potently inhibits the ubiquitin–proteasome system (UPS) via targeting the 26 S proteasome-associated deubiquitinases (DUBs), without effecting on the 20 S proteasome. Here we further report that (i) PtPT induces apoptosis in Bcr-Abl wild-type and Bcr-Abl-T315I mutation cells including the primary mononuclear cells from CML patients clinically resistant to IM, as well as inhibits the growth of IM-resistant Bcr-Abl-T315I xenografts in vivo; (ii) PtPT downregulates Bcr-Abl level through restraining Bcr-Abl transcription, and decreasing Bcr-Abl protein mediated by DUBs inhibition-induced caspase activation; (iii) UPS inhibition is required for PtPT-induced caspase activation and cell apoptosis. These findings support that PtPT overcomes IM resistance through both Bcr-Abl-dependent and -independent mechanisms. We conclude that PtPT can be a lead compound for further drug development to overcome imatinib resistance in CML patients.
Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain
Authors: Song Dan-Dan , Zhang Tong-Tong , Chen Jia-Li , Xia Yun-Fei , Qin Zheng-Hong , Waeber Christian , Sheng Rui 
Published: July 2017

AbstractOur previous findings suggest that sphingosine kinase 2 (SPK2) mediates ischemic tolerance and autophagy in cerebral preconditioning. The aim of this study was to determine by which mechanism SPK2 activates autophagy in neural cells. In both primary murine cortical neurons and HT22 hippocampal neuronal cells, overexpression of SPK2 increased LC3II and enhanced the autophagy flux. SPK2 overexpression protected cortical neurons against oxygen glucose deprivation (OGD) injury, as evidenced by improvement of neuronal morphology, increased cell viability and reduced lactate dehydrogenase release. The inhibition of autophagy effectively suppressed the neuroprotective effect of SPK2. SPK2 overexpression reduced the co-immunoprecipitation of Beclin-1 and Bcl-2, while Beclin-1 knockdown inhibited SPK2-induced autophagy. Both co-immunoprecipitation and GST pull-down analysis suggest that SPK2 directly interacts with Bcl-2. SPK2 might interact to Bcl-2 in the cytoplasm. Notably, an SPK2 mutant with L219A substitution in its putative BH3 domain was not able to activate autophagy. A Tat peptide fused to an 18-amino acid peptide encompassing the native, but not the L219A mutated BH3 domain of SPK2 activated autophagy in neural cells. The Tat-SPK2 peptide also protected neurons against OGD injury through autophagy activation. These results suggest that SPK2 interacts with Bcl-2 via its BH3 domain, thereby dissociating it from Beclin-1 and activating autophagy. The observation that Tat-SPK2 peptide designed from the BH3 domain of SPK2 activates autophagy and protects neural cells against OGD injury suggest that this structure may provide the basis for a novel class of therapeutic agents against ischemic stroke.
Conservation and divergence of mitochondrial apoptosis pathway in the Pacific oyster, Crassostrea gigas
Authors: Li Yingxiang , Zhang Linlin , Qu Tao , Tang Xueying , Li Li , Zhang Guofan 
Published: July 2017

AbstractApoptosis is considered a crucial part of the host defense system in oysters according to previous reports; however, the exact process by which this occurs remains unclear. Besides, mitochondrial apoptosis is the primary method of apoptosis in vertebrate cells, but has been poorly studied in invertebrates and is quite controversial. In this study, we investigated the molecular mechanism of mitochondrial apoptosis in the Pacific oyster Crassostrea gigas. Notably, we show that most key elements involved in the vertebrate mitochondrial apoptosis pathway – including mitochondrial outer membrane permeabilization, cytochrome c release, and caspase activation – are also present in C. gigas. In contrast, the lack of Bcl-2 homology 3-only subfamily members and apoptotic protease activating factor-1 (APAF-1) protein revealed evolutionary diversity from other phyla. Our results support that mitochondrial apoptosis in animals predates the emergence of vertebrates, but suggest that an unexpectedly diverse mitochondrial apoptosis pathway may exist in invertebrates. In addition, our work provided new clues for an improved understanding of how bivalve acclimate themselves to an inconstant environment.
MIS-11 duration key to disappearance of the Greenland ice sheet
Authors: Robinson Alexander , Alvarez-Solas Jorge , Calov Reinhard , Ganopolski Andrey , Montoya Marisa 
Published: July 2017

AbstractPalaeo data suggest that Greenland must have been largely ice free during Marine Isotope Stage 11 (MIS-11). However, regional summer insolation anomalies were modest during this time compared to MIS-5e, when the Greenland ice sheet likely lost less volume. Thus it remains unclear how such conditions led to an almost complete disappearance of the ice sheet. Here we use transient climate–ice sheet simulations to simultaneously constrain estimates of regional temperature anomalies and Greenland’s contribution to the MIS-11 sea-level highstand. We find that Greenland contributed 6.1 m (3.9–7.0 m, 95% credible interval) to sea level, ∼7 kyr after the peak in regional summer temperature anomalies of 2.8 °C (2.1–3.4 °C). The moderate warming produced a mean rate of mass loss in sea-level equivalent of only around 0.4 m per kyr, which means the long duration of MIS-11 interglacial conditions around Greenland was a necessary condition for the ice sheet to disappear almost completely.
YAP determines the cell fate of injured mouse hepatocytes in vivo
Authors: Miyamura Norio , Hata Shoji , Itoh Tohru , Tanaka Minoru , Nishio Miki , Itoh Michiko , Ogawa Yoshihiro , Terai Shuji , Sakaida Isao , Suzuki Akira , Miyajima Atsushi , Nishina Hiroshi 
Published: July 2017

AbstractThe presence of senescent, transformed or damaged cells can impair tissue function or lead to tumorigenesis; therefore, organisms have evolved quality control mechanisms to eliminate them. Here, we show that YAP activation induced by inactivation of the Hippo pathway specifically in damaged hepatocytes promotes their selective elimination by using in vivo mosaic analysis in mouse liver. These damaged hepatocytes migrate into the hepatic sinusoids, undergo apoptosis and are engulfed by Kupffer cells. In contrast, YAP activation in undamaged hepatocytes leads to proliferation. Cellular stresses such as ethanol that damage both liver sinusoidal endothelial cells and hepatocytes switch cell fate from proliferation to migration/apoptosis in the presence of activated YAP. This involves the activation of CDC42 and Rac that regulate cell migration. Thus, we suggest that YAP acts as a stress sensor that induces elimination of injured cells to maintain tissue and organ homeostasis.
Ballistic superconductivity in semiconductor nanowires
Authors: Zhang Hao , Gül Önder , Conesa-Boj Sonia , Nowak Michał P. , Wimmer Michael , Zuo Kun , Mourik Vincent , de Vries Folkert K. , van Veen Jasper , de Moor Michiel W. A. , Bommer Jouri D. S. , van Woerkom David J. , Car Diana , Plissard Sébastien R , Bakkers Erik P.A.M. , Quintero-Pérez Marina , Cassidy Maja C. , Koelling Sebastian , Goswami Srijit , Watanabe Kenji , Taniguchi Takashi , Kouwenhoven Leo P. 
Published: July 2017

AbstractSemiconductor nanowires have opened new research avenues in quantum transport owing to their confined geometry and electrostatic tunability. They have offered an exceptional testbed for superconductivity, leading to the realization of hybrid systems combining the macroscopic quantum properties of superconductors with the possibility to control charges down to a single electron. These advances brought semiconductor nanowires to the forefront of efforts to realize topological superconductivity and Majorana modes. A prime challenge to benefit from the topological properties of Majoranas is to reduce the disorder in hybrid nanowire devices. Here we show ballistic superconductivity in InSb semiconductor nanowires. Our structural and chemical analyses demonstrate a high-quality interface between the nanowire and a NbTiN superconductor that enables ballistic transport. This is manifested by a quantized conductance for normal carriers, a strongly enhanced conductance for Andreev-reflecting carriers, and an induced hard gap with a significantly reduced density of states. These results pave the way for disorder-free Majorana devices.
Near-infrared autofluorescence induced by intraplaque hemorrhage and heme degradation as marker for high-risk atherosclerotic plaques
Authors: Htun Nay Min , Chen Yung Chih , Lim Bock , Schiller Tara , Maghzal Ghassan J. , Huang Alex L. , Elgass Kirstin D. , Rivera Jennifer , Schneider Hans G. , Wood Bayden R. , Stocker Roland , Peter Karlheinz 
Published: July 2017

AbstractAtherosclerosis is a major cause of mortality and morbidity, which is mainly driven by complications such as myocardial infarction and stroke. These complications are caused by thrombotic arterial occlusion localized at the site of high-risk atherosclerotic plaques, of which early detection and therapeutic stabilization are urgently needed. Here we show that near-infrared autofluorescence is associated with the presence of intraplaque hemorrhage and heme degradation products, particularly bilirubin by using our recently created mouse model, which uniquely reflects plaque instability as seen in humans, and human carotid endarterectomy samples. Fluorescence emission computed tomography detecting near-infrared autofluorescence allows in vivo monitoring of intraplaque hemorrhage, establishing a preclinical technology to assess and monitor plaque instability and thereby test potential plaque-stabilizing drugs. We suggest that near-infrared autofluorescence imaging is a novel technology that allows identification of atherosclerotic plaques with intraplaque hemorrhage and ultimately holds promise for detection of high-risk plaques in patients.
Perfluorooctane sulfonate affects intestinal immunity against bacterial infection
Authors: Suo Caixia , Fan Zhiqin , Zhou Liang , Qiu Ju 
Published: July 2017

AbstractPerfluorooctane sulfonate (PFOS) is an environmental contaminant that has been manufactured to be used as surfactants and repellents in industry. Due to long half-life for clearance and degradation, PFOS is accumulative in human body and has potential threat to human health. Previous studies have shown the development and function of immune cells can be affected by PFOS. Although PFOS has a high chance of being absorbed through the oral route, whether and how PFOS affects immune cells in the gut is unknown. Using mouse model of Citrobacter rodentium infection, we investigated the role of PFOS on intestinal immunity. We found at early phase of the infection, PFOS inhibited the expansion of the pathogen by promoting IL-22 production from the group 3 innate lymphoid cell (ILC3) in an aryl hydrocarbon receptor dependent manner. Nevertheless, persistent PFOS treatment in mice finally led to a failure to clear the pathogen completely. At late phase of infection, enhanced bacterial counts in PFOS treated mice were accompanied by increased inflammatory cytokines, reduced mucin production and dysbiosis, featured by decreased level of Lactobacillus casei, Lactobacillus johnsonii and increased E. coli. Our study reveals a deleterious consequence in intestinal bacterial infection caused by PFOS accumulation.
Identification for Differential Localization of Putative Corneal Epithelial Stem Cells in Mouse and Human
Authors: Li Jin , Xiao Yangyan , Coursey Terry G. , Chen Xin , Deng Ruzhi , Lu Fan , Pflugfelder Stephen C. , Li De-Quan 
Published: July 2017

AbstractHuman Corneal epithelial stem cells (CESCs) have been identified to reside in limbus for more than 2 decades. However, the precise location of CESCs in other mammalian remains elusive. This study was to identify differential localization of putative CESCs in mice. Through a series of murine corneal cross-sections from different directions, we identified that anatomically and morphologically the murine limbus is composed of the thinnest epithelium and the thinnest stroma without any palisades of Vogt-like niche structure. The cells expressing five of stem/progenitor cell markers are localized in basal layer of entire murine corneal epithelium. BrdU label-retaining cells, a key characteristic of epithelial stem cells, are detected in both limbal and central cornea of mouse eye. Functionally, corneal epithelium can be regenerated in cultures from central and limbal explants of murine cornea. Such a distribution of mouse CESCs is different from human cornea, where limbal stem cell concept has been well established and accepted. We are aware that some new evidence supports limbal stem cell concept in mouse recently. However, it is important to know that central cornea may provide an alternative source of stem cells when one utilizes mice as animal model for corneal research.