bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selbstberichtskalen zur Erfassung motivationsbezogener Kompetenzen (MOBEKO) im Studium
Authors: Schaller Patrick , Spinath Birgit 
Published: March 2017

Zusammenfassung. Motivationale Defizite gelten als wichtige Ursache von problematischem Arbeitsverhalten und Minderleistung von Studierenden. Der vorliegende Beitrag beschreibt eine Konzeption von Motivation als Kompetenz, die zwischen motivationalen (MOKO), volitionalen (VOKO) und bewertenden (BEKO) Kompetenzen unterscheidet. Zu deren Diagnose wurden Skalen zur Erfassung motivationsbezogener Kompetenzen (MOBEKO) bei Studierenden entwickelt, welche anhand einer Stichprobe von N = 620 Studierenden validiert wurden. Konfirmatorische Faktorenanalysen lieferten Belege für die theoretisch angenommene Struktur. Es konnte gezeigt werden, dass die MOBEKO-Skalen mit gleichzeitig erhobenen anderen Konstrukten theoretisch sinnvolle Zusammenhänge aufwiesen und auch zu einem zweiten Messzeitpunkt erhobene, situationsspezifische Kriterien vorhersagten. Insgesamt liefern die Ergebnisse Belege dafür, dass mit den MOBEKO-Skalen eine objektive, reliable und valide Messung von selbst eingeschätzten motivationsbezogenen Kompetenzen möglich ist.
Assessment of the Psychometric Properties of the Drinking Motives Questionnaire – Revised Among Irish Drinkers
Authors: Regan Daniel , McCaffrey John , Walsh Chloe , MacNeela Padraig , Morrison Todd G. 
Published: March 2017

Abstract. Motives ascribed to drinking represent an important area of investigation in alcohol research. The most commonly used measure is the 20-item Drinking Motives Questionnaire – Revised (DMQ-R: Cooper, 1994 ), which assesses four motives: Enhancement, Social, Coping, and Conformity. Although researchers in Europe have begun to assess the DMQ-R, to date, no published assessment has been undertaken among English-speaking, non-American samples. The current study addressed this omission by conducting exploratory ( N = 437) and confirmatory ( Ns = 437 and 344) factor analyses with Irish participants. A three-factor solution was optimal: Coping (four items), Conformity (five items), and Positive Motives (seven items). The need to conduct culturally specific psychometric testing is discussed as are directions for future research.
When Work Takes Over
Authors: Wiese Bettina S. , Heidemeier Heike , Burk Christian L. , Freund Alexandra M. 
Published: March 2017

Abstract. This study investigates whether two specific strategies of emotional labor (surface and deep acting) shown during client interactions are associated with work-related intrusions on thoughts outside of work. N = 157 bank employees provided reports of emotional labor and of work-related rumination at home on five consecutive workdays. Converging with expectations, we found that on days when surface acting was used, participants reported higher rumination. In contrast, deep acting had no significant association with work-related ruminations at home.
Quantum correlation enhanced super-resolution localization microscopy enabled by a fibre bundle camera
Authors: Israel Yonatan , Tenne Ron , Oron Dan , Silberberg Yaron 
Published: March 2017

AbstractDespite advances in low-light-level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of subdiffraction-limited imaging utilizing quantum statistics of photons, have remained in the realm of proof-of-principle demonstrations. This is primarily due to a combination of low values of fill factors, quantum efficiencies, frame rates and signal-to-noise characteristic of most available single-photon sensitive imaging detectors. Here we describe an imaging device based on a fibre bundle coupled to single-photon avalanche detectors that combines a large fill factor, a high quantum efficiency, a low noise and scalable architecture. Our device enables localization-based super-resolution microscopy in a non-sparse non-stationary scene, utilizing information on the number of active emitters, as gathered from non-classical photon statistics.
CCR5 promoter activity correlates with HIV disease progression by regulating CCR5 cell surface expression and CD4 T cell apoptosis
Authors: Joshi Anjali , Punke Erin B. , Sedano Melina , Beauchamp Bethany , Patel Rima , Hossenlopp Cassady , Alozie Ogechika K. , Gupta Jayanta , Mukherjee Debabrata , Garg Himanshu 
Published: March 2017

AbstractCCR5 is the major co-receptor for HIV and polymorphisms in the CCR5 gene as well as promoter region that alter cell surface expression have been associated with disease progression. We determined the relationship between CCR5 promoter polymorphisms and CD4 decline and other immunopathological features like immune activation and CD4+ T cell apoptosis in HIV patients. CCR5 promoter haplotype HHC was significantly associated with higher CD4 counts in patients. The relative promoter activity (RPA) of each haplotype was determinedin vitroand combined promoter activity based on both alleles (CRPA) was assigned to each patients. Interestingly, CCR5 CRPA correlated inversely with CD4 counts and CD4:CD8 ratio specifically in viremic patients. In normal individuals, the CRPA correlated with the number of CCR5+ CD4+ T cells in the peripheral blood suggesting an effect on CCR5 expression. In a subset of high viremic patients harboring R5 tropic HIV, there was a strong correlation between CCR5 CRPA and both CD4 counts and CD4 T cell apoptosis. Our study demonstrates that, CCR5 promoter polymorphisms correlate with CD4 T cell loss possibly by regulating CD4 T cell apoptosis in HIV patients. Furthermore, assigning CRPAs to each patient is a new method of translating genotype to phenotype.
Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Manila clam Ruditapes philippinarum
Authors: Nie Hongtao , Yan Xiwu , Huo Zhongming , Jiang Liwen , Chen Peng , Liu Hui , Ding Jianfeng , Yang Feng 
Published: March 2017

AbstractGenetic linkage maps are indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density linkage maps has become achievable in marine organisms lacking sufficient genomic resources, such as mollusks. In this study, high-density linkage map was constructed for an ecologically and commercially important clam species,Ruditapes philippinarum. For the consensus linkage map, a total of 9658 markers spanning 1926.98 cM were mapped to 18 sex-averaged linkage groups, with an average marker distance of 0.42 cM. Based on the high-density linkage map, ten QTLs for growth-related traits and shell color were detected. The coverage and density of the current map are sufficient for us to effectively detect QTL for segregating traits, and two QTL positions were all coincident with the closest markers. This high-density genetic linkage map reveals basic genomic architecture and will be useful for comparative genomics research, genome assembly and genetic improvement ofR. philippinarumand other bivalve molluscan species.
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Authors: Moen Spencer O. , Edwards Thomas E. , Dranow David M. , Clifton Matthew C. , Sankaran Banumathi , Van Voorhis Wesley C. , Sharma Amit , Manoil Colin , Staker Bart L. , Myler Peter J. , Lorimer Donald D. 
Published: March 2017

AbstractAminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline. Here we investigate thirty-one aminoacyl-tRNA synthetases from infectious disease organisms by co-crystallization in the presence of their cognate amino acid, ATP, and/or inhibitors. Crystal structures were determined for a CysRS from Borrelia burgdorferi bound to AMP, GluRS from Borrelia burgdorferi and Burkholderia thailandensis bound to glutamic acid, a TrpRS from the eukaryotic pathogen Encephalitozoon cuniculi bound to tryptophan, a HisRS from Burkholderia thailandensis bound to histidine, and a LysRS from Burkholderia thailandensis bound to lysine. Thus, the presence of ligands may promote aaRS crystallization and structure determination. Comparison with homologous structures shows conformational flexibility that appears to be a recurring theme with this enzyme class.
Surface Crystallization of a MgO/Y2O3/SiO2/Al2O3/ZrO2 Glass: Growth of an Oriented β-Y2Si2O7 Layer and Epitaxial ZrO2
Authors: Wisniewski Wolfgang , Seidel Sabrina , Patzig Christian , Rüssel Christian 
Published: March 2017

AbstractThe crystallization behavior of a glass with the composition 54.7 SiO2·10.9 Al2O3·15.0 MgO·3.4 ZrO2·16.0 Y2O3 is studied using X-ray diffraction (XRD), scanning electron microscopy (SEM) including electron backscatter diffraction (EBSD) and (scanning) transmission electron microscopy [(S)TEM] including energy-dispersive X-ray spectrometry (EDXS). This glass shows the sole surface crystallization of four different yttrium silicates of the composition Y2Si2O7 (YS). The almost simultaneous but independent nucleation of α-, β-, δ-, and ε-YS at the surface is followed by growth into the bulk, where ε-YS quickly dominates a first crystallized layer. An accumulation of Mg at the growth front probably triggers a secondary nucleation of β-YS, which forms a thin compact layer before fragmenting into a highly oriented layer of fine grained crystals occupying the remaining bulk. The residual glass between the YS growth structures allows the crystallization of indialite, yttrium stabilized ZrO2 (Y-ZrO2) and very probably μ-cordierite during cooling. Hence, this glass basically shows the inverted order of crystallization observed in other magnesium yttrium alumosilicate glasses containing less Y2O3. An epitaxial relationship between Y-ZrO2 and ε-YS is proven and multiple twinning relationships occur in the YS phases.
Formulation for Oral Delivery of Lactoferrin Based on Bovine Serum Albumin and Tannic Acid Multilayer Microcapsules
Authors: Kilic Ece , Novoselova Marina V. , Lim Su Hui , Pyataev Nikolay A. , Pinyaev Sergey I. , Kulikov Oleg A. , Sindeeva Olga A. , Mayorova Oksana A. , Murney Regan , Antipina Maria N. , Haigh Brendan , Sukhorukov Gleb B. , Kiryukhin Maxim V. 
Published: March 2017

AbstractLactoferrin (Lf) has considerable potential as a functional ingredient in food, cosmetic and pharmaceutical applications. However, the bioavailability of Lf is limited as it is susceptible to digestive enzymes in gastrointestinal tract. The shells comprising alternate layers of bovine serum albumin (BSA) and tannic acid (TA) were tested as Lf encapsulation system for oral administration. Lf absorption by freshly prepared porous 3 μm CaCO3 particles followed by Layer-by-Layer assembly of the BSA-TA shells and dissolution of the CaCO3 cores was suggested as the most efficient and harmless Lf loading method. The microcapsules showed high stability in gastric conditions and effectively protected encapsulated proteins from digestion. Protective efficiency was found to be 76 ± 6% and 85 ± 2%, for (BSA-TA)4 and (BSA-TA)8 shells, respectively. The transit of Lf along the gastrointestinal tract (GIT) of mice was followed in vivo and ex vivo using NIR luminescence. We have demonstrated that microcapsules released Lf in small intestine allowing 6.5 times higher concentration than in control group dosed with the same amount of free Lf. Significant amounts of Lf released from microcapsules were then absorbed into bloodstream and accumulated in liver. Suggested encapsulation system has a great potential for functional foods providing lactoferrin.
EP2 receptor antagonism reduces peripheral and central hyperalgesia in a preclinical mouse model of endometriosis
Authors: Greaves Erin , Horne Andrew W. , Jerina Helen , Mikolajczak Marta , Hilferty Lisa , Mitchell Rory , Fleetwood-Walker Sue M. , Saunders Philippa T. K. 
Published: March 2017

AbstractEndometriosis is an incurable gynecological disorder characterized by debilitating pain and the establishment of innervated endometriosis lesions outside the uterus. In a preclinical mouse model of endometriosis we demonstrated overexpression of the PGE2-signaling pathway (including COX-2, EP2, EP4) in endometriosis lesions, dorsal root ganglia (DRG), spinal cord, thalamus and forebrain. TRPV1, a PGE2-regulated channel in nociceptive neurons was also increased in the DRG. These findings support the concept that an amplification process occurs along the pain neuroaxis in endometriosis. We then tested TRPV1, EP2, and EP4 receptor antagonists: The EP2 antagonist was the most efficient analgesic, reducing primary hyperalgesia by 80% and secondary hyperalgesia by 40%. In this study we demonstrate reversible peripheral and central hyperalgesia in mice with induced endometriosis.
Oral bisphosphonates and colorectal cancer
Authors: Vogtmann Emily , Corley Douglas A. , Almers Lucy M. , Cardwell Chris R. , Murray Liam J. , Abnet Christian C. 
Published: March 2017

AbstractUse of oral bisphosphonates has been associated with a decreased risk of colorectal cancer (CRC), but the association may be related to residual confounding by healthy lifestyle or body mass index (BMI). Therefore, we conducted a prospective nested case-control study within the Kaiser Permanente, Northern California health system cohort. In total, 12,505 CRC cases were individually matched to 599,534 controls. Odds ratios (OR) and 95% confidence intervals (CI) were calculated using conditional logistic regression models with adjustment for important covariates extracted from the database. Participants who had ever used oral bisphosphonates were less likely than non-users to be diagnosed with CRC (OR 0.82; 95% CI: 0.74, 0.89). Colon and rectum site-specific associations were similar to the overall association. A stronger inverse association for ever use of bisphosphonates was observed for men (OR 0.63; 95% CI: 0.47, 0.85), however when stratified by previous lower endoscopy, the association was only observed in the participants who did not have a previous lower endoscopy (OR 0.73 (0.64, 0.83)). In conclusion, we found that oral bisphosphonate use was associated with a decreased odds of CRC, however this association may be due to residual confounding by BMI or another confounder.
Infiltrated bunch of solitons in Bi-doped frequency-shifted feedback fibre laser operated at 1450 nm
Authors: Rissanen Joona , Korobko Dmitry A. , Zolotovsky Igor O. , Melkumov Mikhail , Khopin Vladimir F. , Gumenyuk Regina 
Published: March 2017

AbstractMode-locked fibre laser as a dissipative system is characterized by rich forms of soliton interaction, which take place via internal energy exchange through noisy background in the presence of dispersion and nonlinearity. The result of soliton interaction was either stationary-localized or chaotically-oscillated soliton complexes, which have been shown before as stand-alone in the cavity. Here we report on a new form of solitons complex observed in Bi-doped mode-locked fibre laser operated at 1450 nm. The solitons are arranged in two different group types contemporizing in the cavity: one pulse group propagates as bound solitons with fixed phase relation and interpulse position eventuated in 30 dB spectrum modulation depth; while the other pulses form a bunch with continuously and chaotically moving solitons. The article describes both experimental and theoretical considerations of this effect.
A high-density SNP genetic map consisting of a complete set of homologous groups in autohexaploid sweetpotato (Ipomoea batatas)
Authors: Shirasawa Kenta , Tanaka Masaru , Takahata Yasuhiro , Ma Daifu , Cao Qinghe , Liu Qingchang , Zhai Hong , Kwak Sang-Soo , Cheol Jeong Jae , Yoon Ung-Han , Lee Hyeong-Un , Hirakawa Hideki , Isobe Sachiko 
Published: March 2017

AbstractSweetpotato (Ipomoea batatas) is an autohexaploid species with 90 chromosomes (2n = 6x = 90) and a basic chromosome number of 15, and is therefore regarded as one of the most challenging species for high-density genetic map construction. Here, we used single nucleotide polymorphisms (SNPs) identified by double-digest restriction site-associated DNA sequencing based on next-generation sequencing technology to construct a map for sweetpotato. We then aligned the sequence reads onto the reference genome sequence of I. trifida, a likely diploid ancestor of sweetpotato, to detect SNPs. In addition, to simplify analysis of the complex genetic mode of autohexaploidy, we used an S1 mapping population derived from self-pollination of a single parent. As a result, 28,087 double-simplex SNPs showing a Mendelian segregation ratio in the S1 progeny could be mapped onto 96 linkage groups (LGs), covering a total distance of 33,020.4 cM. Based on the positions of the SNPs on the I. trifida genome, the LGs were classified into 15 groups, each with roughly six LGs and six small extra groups. The molecular genetic techniques used in this study are applicable to high-density mapping of other polyploid plant species, including important crops.
Multicolor emission from intermediate band semiconductor ZnO1−xSex
Authors: Welna M. , Baranowski M. , Linhart W. M. , Kudrawiec R. , Yu K. M. , Mayer M. , Walukiewicz W. 
Published: March 2017

AbstractPhotoluminescence and photomodulated reflectivity measurements of ZnOSe alloys are used to demonstrate a splitting of the valence band due to the band anticrossing interaction between localized Se states and the extended valence band states of the host ZnO matrix. A strong multiband emission associated with optical transitions from the conduction band to lower E− and upper E+ valence subbands has been observed at room temperature. The composition dependence of the optical transition energies is well explained by the electronic band structure calculated using the kp method combined with the band anticrossing model. The observation of the multiband emission is possible because of relatively long recombination lifetimes. Longer than 1 ns lifetimes for holes photoexcited to the lower valence subband offer a potential of using the alloy as an intermediate band semiconductor for solar power conversion applications.
Roles of vacuum tunnelling and contact mechanics in single-molecule thermopower
Authors: Tsutsui Makusu , Yokota Kazumichi , Morikawa Takanori , Taniguchi Masateru 
Published: March 2017

AbstractMolecular junction is a chemically-defined nanostructure whose discrete electronic states are expected to render enhanced thermoelectric figure of merit suitable for energy-harvesting applications. Here, we report on geometrical dependence of thermoelectricity in metal-molecule-metal structures. We performed simultaneous measurements of the electrical conductance and thermovoltage of aromatic molecules having different anchoring groups at room temperature in vacuum. We elucidated the mutual contributions of vacuum tunnelling on thermoelectricity in the short molecular bridges. We also found stretching-induced thermoelectric voltage enhancement in thiol-linked single-molecule bridges along with absence of the pulling effects in diamine counterparts, thereby suggested that the electromechanical effect would be a rather universal phenomenon in Au-S anchored molecular junctions that undergo substantial metal-molecule contact elongation upon stretching. The present results provide a novel concept for molecular design to achieve high thermopower with single-molecule junctions.
Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis
Authors: Boenisch Marike Johanne , Broz Karen Lisa , Purvine Samuel Owen , Chrisler William Byron , Nicora Carrie Diana , Connolly Lanelle Reine , Freitag Michael , Baker Scott Edward , Kistler Harold Corby 
Published: March 2017

AbstractCompartmentalization of metabolic pathways to particular organelles is a hallmark of eukaryotic cells. Knowledge of the development of organelles and attendant pathways under different metabolic states has been advanced by live cell imaging and organelle specific analysis. Nevertheless, relatively few studies have addressed the cellular localization of pathways for synthesis of fungal secondary metabolites, despite their importance as bioactive compounds with significance to medicine and agriculture. When triggered to produce sesquiterpene (trichothecene) mycotoxins, the endoplasmic reticulum (ER) of the phytopathogenic fungus Fusarium graminearum is reorganized both in vitro and in planta. Trichothecene biosynthetic enzymes accumulate in organized smooth ER with pronounced expansion at perinuclear- and peripheral positions. Fluorescence tagged trichothecene biosynthetic proteins co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. We hypothesize that changes to the fungal ER represent a conserved process in specialized eukaryotic cells such as in mammalian hepatocytes and B-cells.
NLRX1 accelerates cisplatin-induced ototoxity in HEI-OC1 cells via promoting generation of ROS and activation of JNK signaling pathway
Authors: Yin Haiyan , Sun Gaoying , Yang Qianqian , Chen Chen , Qi Qi , Wang Haibo , Li Jianfeng 
Published: March 2017

AbstractNucleotide-binding domain and leucine-rich-repeat-containing family member X1 (NLRX1), located in mitochondria, can recognize cytoplasmic pattern recognition receptors and is tightly related to reactive oxygen species (ROS) production, mitochondrial function, apoptosis and inflammation. The present study was designed to explore whether NLRX1 expresses in HEI-OC1 cells and, if so, to investigate the possible correlations between NLRX1 and cisplatin-induced ototoxity in vitro. Here, we report that NLRX1 was specifically localized to mitochondria in the cytoplasm of HEI-OC1 cells and its expression was increased concurrent with the increase of ROS production and occurrence of apoptosis in HEI-OC1 cells in response to cisplatin stimulus. NLRX1 overexpression led to a higher apoptosis in HEI-OC1 cells treated with cisplatin, whereas, NLRX silencing decreased cisplatin induced apoptosis. Mechanistic studies showed that NLRX1 activated mitochondrial apoptosis pathway as well as promoted ROS generation and JNK activation. Either inhibition of ROS generation or JNK signaling significantly prevented NLRX1-mediated mitochondrial apoptosis in HEI-OC1cells. In addition, NLRX1 expression was confirmed in cochlear explants. The findings from this work reveal that NLRX1 sensitizes HEI-OC1 cells to cisplatin-induced apoptosis via activation of ROS/JNK signaling pathway, suggesting that NLRX1 acts as an important regulator of the cisplatin-elicited ototoxity.