bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased serum human epididymis protein 4 is associated with disease activity and systemic involvement in pediatric-onset systemic lupus erythematosus
Authors: Liu Chenxi , Yan Lingyi , Zhang Menglan , Duan Yifei , Luo Jie , Liu Zhijun , Liu Ting , Tong Jiyu , Jiang Yongmei 
Published: September 2024

ObjectiveWe aimed to investigate human epididymis protein 4 (HE4) as a potential biomarker in patients with pediatric-onset systemic lupus erythematosus (pSLE), particularly on the association of serum HE4 levels with disease activity and other laboratory tests.MethodsWe included 137 patients with pSLE and 75 age- and sex-matched healthy controls (HCs). Serum HE4 level was measured by a chemiluminescent microparticle on an Abbott ARCHITECT i2000SR Immunoassay Analyzer. Comparisons between groups were performed using the independent Student t-test, Mann–Whitney U test, Chi-square test, or Fisher’s exact test, as appropriate. We also determined the relationships between HE4 and clinical parameters and evaluated disease activity using SLE Disease Activity Index (SLEDAI) and renal SLEDAI (rSLEDAI).ResultsSerum HE4 levels in patients with pSLE (44.6 pmol/L; IQR, 32.5–73.5) were significantly higher than those in HCs (38.9 pmol/L; IQR, 34–46.1). HE4 levels were significantly higher in moderate to severe disease activities (57.4 pmol/L, IQR 37.7–164.5) than in mild disease activities (38.8 pmol/L, IQR 30.1–48.5) or HCs (38.9 pmol/L, IQR 34.0–46.1), as well as in active renal disease activities (77.2 pmol/L, IQR 47.4–224.1) than in inactive renal disease activities (36.1 pmol/L, IQR 27.8–46.7). The ROC curve analysis showed that HE4 could discriminate pSLE with renal (AUC, 0.717; 95% CI, 0.632–0.801), hematological (AUC, 0.740; 95% CI, 0.648–0.831), and cardiovascular involvement (AUC:0.775, 95% CI 0.669–0.880). Serum HE4 levels significantly correlated with several indicators related to renal morbidity, such as creatinine, blood urea nitrogen, uric acid, cystatin C, urine protein/24 h, etc.ConclusionSerum HE4 levels in pSLE were elevated and highly associated with disease activity and systemic involvement, indicating HE4 as a potential biomarker for pSLE.
Revisiting Thorpe scale analysis and diapycnal diffusivities in Drake Passage
Authors: Park Young-Hyang , Durand Isabelle , Lee Jae-Hak , Provost Christine 
Published: September 2024

Ship heave effects in the swell-prevalent Southern Ocean induce frequent false overturns in density profiles, and conventional Thorpe scale analysis leads to unrealistically large diapycnal diffusivities. Another critical factor causing large positive biases in Thorpe scale analysis concerns the Ozmidov to Thorpe scales ratio α often considered constant and equal to 0.8. A revised Thorpe scale analysis is proposed with an innovative segment-by-segment approach to circumvent heave-induced false overturns together with best-fitting α functions to Diapycnal and Isopycnal Mixing Experiment in the Southern Ocean (DIMES) data, which yields realistic diffusivities in the Drake Passage region. The revised Thorpe scale method applied to a finely-resolved CTD section across the Drake Passage yields typical diffusivities of O (10-5-10-3 m2 s-1), with the largest values being preferentially concentrated close to the bottom at major circumpolar fronts, consistent with nearby DIMES microstructure observations. In the southern Drake Passage the method highlights a middepth (500-1500 m) mixing maximum of 10-4-10-3 m2 s-1 in the Lower Circumpolar Deep Water (LCDW) layer due to large intrusions of Warm Deep Water (a cold/fresh variety of LCDW) of Weddell Gyre origin. The inferred middepth mixing maximum in intrusive regions, which is associated with isopycnal eddy stirring rather than caused by internal waves or double diffusive mixing, supports the short-circuiting paradigm of meridional overturning circulation in the southwestern Scotia Sea region and has important climatic implications in a warming climate.
Discovering the anti-diabetic potential of pomegranate peel metabolites by examining molecular interplay with the thioredoxin-interacting protein
Authors: Aslam Tahira , Arif Amina , Arshad Shafia , Muccee Fatima , Ahmad Khalil , Iqbal Muhammad Omer , Khalil Umair , Razak Suhail , Afsar Tayyaba , Almajwal Ali , Shafique Huma , Zain Maryam 
Published: September 2024

IntroductionMedicinal plants like Punica granatum (pomegranate) have traditional uses against diabetes, inflammation and other diseases. The study was initiated to get an insight into the interaction tendency of P. granatum derived compounds with diabetes associated human thioredoxin-interacting protein (TXNIP). High glucose in diabetes induces production of TXNIP resulting in β-cells apoptosis. Its inhibition might reduce the diabetes incidence.MethodsTo elucidate the therapeutic potential of P. granatum peel against diabetes through GC-MS based identification of extracted compounds followed by application of computational algorithms. P. granatum peel extracts were screened for antioxidant, anti-inflammatory, anti-diabetic, antimicrobial and wound healing properties. Phytochemical and GC-MS based analysis were performed to identify the bioactive compounds. Molecular docking analysis was performed by Auto Dock Vina to predict the binding tendency of P. granatum derived compounds with TXNIP.Results and DiscussionThe peel exhibited antioxidant, anti-inflammatory and anti-diabetic activities, which were attributed to phytochemicals like phenols, tannins and steroids. GC-MS analysis identified 3,5-octadien-2-one, 1H-pyrrole -2,5-dione, Beta-D-lyxofuranoside, 5-O-(beta-D-lyxofuranosyl)-decyl, diethyl phthalate, 9-octadecenoic acid (Z)-, methyl ester, hexadecanoic acid, methyl ester, n-hexadecanoic acid, tetradecane, 2,6,10-trimethyl, bis (2-ethylhexyl) phthalate, decane, 3,8-dimethyl, 9-octadecenoic acid (Z)-, methyl ester and bis (2-ethylhexyl) phthalate in P. granatum peel extracts. Docking analysis revealed high binding affinities of bis (2-ethylhexyl) phthalate and 9-octadecenoic acid with TXNIP i.e., –4.5 and –5.0 kcal/mol, respectively, reflecting these compounds as potent antidiabetic agents. This study validates the traditional uses of P. granatum peel and demonstrates how computational approaches can uncover pharmacologically active phytochemicals. The results suggest P. granatum peel is a promising source of novel therapeutics against diabetes, inflammation, and oxidation. Further studies on the optimization of identified ligands are warranted.
Integrative metagenomic and lipidomic analyses reveal alterations in children with obesity and after lifestyle intervention
Authors: Yin Chunyan , Liu Lujie , Xu Dong , Li Meng , Li Min , Qin Yujie , Zhang Bei , Sun Yongfa , Liu Yuesheng , Xiao Yanfeng 
Published: September 2024

BackgroundDespite emerging evidence linking alterations in gut microbiota to childhood obesity, the metabolic mechanisms linking gut microbiota to the lipid profile during childhood obesity and weight loss remain poorly understood.MethodologyIn this study, children with obesity were treated with lifestyle weight loss therapy. Metagenomics association studies and serum untargeted lipidomics analyses were performed in children with obesity and healthy controls before and after weight loss.Main findingsWe identified alterations in gut microbiota associated with childhood obesity, as well as variations in circulating metabolite concentrations. Children with obesity showed significant decreases in the levels of s-Rothia_kristinae and s-Enterobacter_roggenkampii, alongsige elevated levels of s-Clostridiales_bacterium_Marseille-P5551. Following weight loss, the levels of s-Streptococcus_infantarius and s-Leuconostoc_citreum increased by factors of 3.354 and 1.505, respectively, in comparison to their pre-weight loss levels. Correlation analyses indicated a significant positive relationship between ChE(2:0) levels and both with s-Lachnospiraceae_bacterium_TF09-5 and fasting glucose levels. CoQ8 levels were significantly negatively correlated with s-Rothia_kristinae and HOMA-IR.ConclusionWe linked altered gut microbiota and serum lipid levels in children with obesity to clinical indicators, indicating a potential impact on glucose metabolism via lipids. This study contributes to understanding the mechanistic relationship between altered gut microbiota and childhood obesity and weight loss, suggesting gut microbiome as a promising target for intervention.Clinical trial registrationhttps://www.chictr.org.cn/showproj.html?proj=178971, ChiCTR2300072179.
Castration-resistant prostate cancer monitoring by cell-free circulating biomarkers
Authors: Chrenková Eva , Študentová Hana , Holá Kateřina , Kahounová Zuzana , Hendrychová Romana , Souček Karel , Bouchal Jan 
Published: September 2024

BackgroundProstate cancer is the second leading cause of male cancer-related deaths in Western countries, which is predominantly attributed to the metastatic castration-resistant stage of the disease (CRPC). There is an urgent need for better prognostic and predictive biomarkers, particularly for androgen receptor targeted agents and taxanes.MethodsWe have searched the PubMed database for original articles and meta-analyses providing information on blood-based markers for castration-resistant prostate cancer monitoring, risk group stratification and prediction of therapy response.ResultsThe molecular markers are discussed along with the standard clinical parameters, such as prostate specific antigen, lactate dehydrogenase or C-reactive protein. Androgen receptor (AR) alterations are commonly associated with progression to CRPC. These include amplification of AR and its enhancer, point mutations and splice variants. Among DNA methylations, a novel 5-hydroxymethylcytosine activation marker of TOP2A and EZH2 has been identified for the aggressive disease. miR-375 is currently the most promising candidate among non-coding RNAs and sphingolipid analysis has recently emerged as a novel approach.ConclusionsThe promising biomarkers have the potential to improve the care of metastatic prostate cancer patients, however, they need further validation for routine implementation.
The congruence between the preferred and actual places of death among terminal cancer patients in China
Authors: Lin Huijing , Ni Ping , Wu Bei , Liao Jing , Fu Jie 
Published: September 2024

BackgroundDying in a preferred place is important for a good death. Currently, no study has evaluated the extent to which the preferences for the place of death (PoD) are met among terminal cancer patients in China. This study examined the congruence between the preferred and actual PoD and its predictors among terminal cancer patients in China.MethodsBetween 2015 and 2023, 845 terminal cancer patients from four tertiary hospitals in Wuhan, China, were enrolled and followed till death. Face-to-face surveys at baseline and telephone-based interviews in the last month of patients’ lives were combined to learn patients’ preferred PoD. Data on patients’ actual PoD were collected from families within 1 month after patients’ death.ResultsOf the 410 patients who died, 62.7% of them died in hospitals. The agreement between patients’ preferred and actual PoD was fair (κ = 0.221). The congruence between patients’ preferred and actual PoD was 63.0, 36.6%, and 0 for hospital death, home death, and hospice facility/nursing home death separately. Patients were more likely to die in their preferred places if their preferred place and family caregivers’ belief of patients’ preferred PoD was congruent (odds ratio [OR] = 6.464, p = 0.001), or if caregivers had a medically related occupation (OR = 4.577, p = 0.003); if patients were hospitalized at least twice in the last month of life (OR = 0.422, p = 0.000), or the quality of care received by patients in the last 48 h was rated good by the families (OR = 0.373, p = 0.011), patients were less likely to die in their preferred places.ConclusionThe congruence between patients’ preferred and actual PoD was fair. Advance care planning (ACP) needs to be popularized in China, and the quality of care in hospice facilities and nursing homes should be improved. The necessary policy support for hospice care should be made to respect cancer patients’ end-of-life (EoL) care preferences in China.
Changes in facial appearance alter one’s sensitivity not only to the self but also to the outside world
Authors: Honma Motoyasu , Yoshiba Sayaka , Miyamoto Saya , Himi Nanae , Haga Shugo , Ogura Sumire , Maki Koutaro , Masaoka Yuri , Izumizaki Masahiko , Shirota Tatsuo 
Published: September 2024

IntroductionChanges in facial appearance due to orthognathic surgery are known to improve a patient’s postoperative quality of life, however, potential changes in cognitive function are unknown. This study examined the effects of changes in facial appearance due to orthognathic surgery on the sensitivity to self and to outside objects in patients with jaw deformities.MethodsPatients with jaw deformities (n = 22) and healthy controls (n = 30) were tested at 3 months preoperatively, at 1 month preoperatively, and at 1 month postoperatively to assess their impression of objects (positive, negative, and neutral pictures) and their evaluation of their own face and body.ResultsThe results showed that changes in facial appearance improved self-evaluation and increased their sensitivity to emotional objects even when the objects were identical. Furthermore, the improving rating for own face was associated with the sensitivity for objects.DiscussionThe changes in facial appearance in patients may have helped to clear the sensitivity to these emotional objects. These findings may provide a new indicator of efficacy in orthognathic surgery.
Experiences of individuals with serious mental disorders in regular employment through the Individual Placement and Support model
Authors: Melián Cartaya María Jesús , Arias Rodríguez Ángeles , Rodríguez Pérez Armando , Sánchez Suárez María , Rodríguez-Novo Natalia , Rodríguez-Novo Yurena , Rodríguez Pulido Francisco 
Published: September 2024

IntroductionThis study shows the perspective, meaning and satisfaction perceived by people with Serious Mental Disorders during their experiences in regular employment.MethodsA mixed qualitative-quantitative methodology was used, applying semi-structured interview as qualitative information collection tool and the Indiana Job Satisfaction Scale as quantitative tool. The study period was from January 2021 to December 2022. A purposive sampling was performed with a sample of 24 people with Serious Mental Disorders who had obtained a job through an Individual Placement and Support (IPS) program during the study period. Semi-structured interview and the Indiana Job Satisfaction Scale were applied to this Serious Mental Disorder workers’ sample. The Indiana Job Satisfaction Scale was also applied to a group of 24 workers without mental disorders in the same working conditions who served as control group.ResultsThe results of the analyses of the Serious Mental Disorder workers’ narratives show that perception of work experience is conditioned by individual, environmental and social predictors, as well as external factors as determining variables. Quantitative results obtained by the Indiana Job Satisfaction Scale reveal levels of job satisfaction resembling those of the rest of workers without Serious Mental Disorder.DiscussionThese findings reinforce the significance of employment in the recovery process for individuals with Serious Mental Disorders and emphasize the importance of understanding the subjective meaning individuals attribute to their work experiences.
A network approach to emotion regulation and symptom activation in depression and anxiety
Authors: Rodrigues Ana Rita , Castro Daniel , Cardoso Joana , Ferreira Filipa , Serrão Carla , Coelho Carlos M. , Meira Liliana , Ferreira Tiago B. 
Published: September 2024

BackgroundEmotions can be regulated through several regulatory strategies that are involved in the development of psychopathological symptoms. Despite the well-established association between psychopathology and emotion dysregulation, little is known about the relationship between individual symptoms of depression and anxiety and emotion regulation strategies (ERS), as well as between ERS themselves.MethodWe conducted a cross-sectional study and examined the interactions between six ERS (reappraisal, engagement, rumination, suppression, arousal control, and distraction) and assessed their distinctive association with the activation of specific symptoms of depression and anxiety in a community sample of 376 adults (80.4% female; Mage = 32.70; SDage = 11.80). The Regulation Emotion Systems Survey (RESS) was used to measure ERS. The Patient Health Questionnaire (PHQ-9) and the Generalized Anxiety Disorder (GAD-7) were used to assess psychological symptoms. An exploratory graph analysis was performed to examine the structural properties of the network of interactions between these behaviors. Additionally, to test the association of ERS with the activation of the depression symptoms network, an expected symptoms activity (ESA) was conducted.ResultsSix communities were found that correspond to the six ERS. Rumination and suppression have a significant association with symptom activation (particularly low self-esteem), whereas reappraisal reduces symptomatic activation. The effect of arousal control, engagement, and distraction appears to depend on the remaining ERS rather than having much influence on their own.ConclusionThis study provides insight into how ERS interact with each other and with individual symptoms of depression and anxiety. Understanding the effects of these interactions on symptom activation and comorbidity can improve our understanding of psychopathology.
Human–animal contact to inform zoonotic disease risk across gradients of agricultural land use change in the Central River Region (CRR) of The Gambia (ZooContact): a formative study
Authors: Ahmed Aliyu N. , Fornace Kimberly , Iwamura Takuya , Murray Kris A. 
Published: September 2024

IntroductionPilot studies are important initial steps in research, providing a preliminary assessment of the practicality, feasibility, and potential challenges of a proposed study. This study attempts to assess the feasibility, practicality, and acceptability of a study that integrates a human–animal contact (HAC) questionnaire, animal biodiversity survey using acoustic analysis, and zoonotic disease investigation in animals among rural households in the Central River Region (CRR) of The Gambia. The pilot study revealed granular insights that would otherwise go unnoticed, providing vital information that directly guided the design and implementation of the subsequent full-scale study on zoonotic disease risk.MethodsA pilot study was conducted in five villages in the CRR of The Gambia. Community sensitization was carried out together with the village leadership, followed by a familiarization tour of the study setting. Questionnaire-based interview was conducted among participants (n = 50) randomly selected to assess the acceptability and reliability of the questionnaire. The feasibility and acceptability of biodiversity surveys and animal sampling were assessed using verbal inquiries from participants and community leaders.ResultsThe recruitment rate was 96%, and most participants, 50 out of 52, were willing to participate without compensation for lost time during interviews. For animal sampling, 45 out of 50 participants were willing to allow the study team to sample blood and feces from their animals without any form of incentive. All five village heads agreed to the usage of sound recorders to be placed within their community for animal biodiversity assessment. For the survey effort, one field assistant interviewed 25 participants per week. It took a total of 1 h to complete an interview, including random household selection, consenting, and questionnaire interview.DiscussionThe pilot study confirmed the feasibility of the research and informed the design of the larger study. Key parameters, such as community access, acceptability, recruitment success, and logistical requirements, contributed to robust sample size calculations and realistic project cost estimates. Additionally, the study enabled the research team to familiarize themselves with the communities and refine the methods for the full study.
Personal characteristics and transmission dynamics associated with SARS-CoV-2 semi-quantitative PCR test results: an observational study from Belgium, 2021–2022
Authors: Braeye Toon , Proesmans Kristiaan , Van Cauteren Dieter , Brondeel Ruben , Hens Niel , Vermeiren Elias , Hammami Naïma , Rosas Angel , Taame Adrae , André Emmanuel , Cuypers Lize 
Published: September 2024

IntroductionFollowing harmonization efforts by the Belgian National Reference Center for SARS-CoV-2, semi-quantitative PCR test (SQ-PCR) results, used as a proxy for viral load, were routinely collected after performing RT-qPCR tests.MethodsWe investigated both the personal characteristics associated with SQ-PCR results and the transmission dynamics involving these results. We used person-level laboratory test data and contact tracing data collected in Belgium from March 2021 to February 2022. Personal characteristics (age, sex, vaccination, and laboratory-confirmed prior infection) and disease stage by date of symptom onset were analyzed in relation to SQ-PCR results using logistic regression. Vaccine effectiveness (VE) against a high viral load (≥107 copies/mL) was estimated from the adjusted probabilities. Contact tracing involves the mandatory testing of high-risk exposure contacts (HREC) after contact with an index case. Odds ratios for test positivity and high viral load in HREC were calculated based on the SQ-PCR result of the index case using logistic regression models adjusted for age, sex, immunity status (vaccination, laboratory-confirmed prior infection), variant (Alpha, Delta, Omicron), calendar time, and contact tracing covariates.ResultsWe included 909,157 SQ-PCR results of COVID-19 cases, 379,640 PCR results from index cases, and 72,052 SQ-PCR results of HREC. High viral load was observed more frequently among recent cases, symptomatic cases, cases over 25 years of age, and those not recently vaccinated (&gt;90 days). The vaccine effectiveness (VE) of the primary schedule in the first 30 days after vaccination was estimated at 47.3% (95%CI 40.8–53.2) during the Delta variant period. A high viral load in index cases was associated with an increased test positivity in HREC (OR 2.7, 95%CI 2.62–2.79) and, among those testing positive, an increased likelihood of a high viral load (OR 2.84, 95%CI 2.53–3.19).