bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRELIMINARY CYTOTOXIC EVALUATION OF Andrographis paniculata IN BREAST CANCER CELL LINES
Authors: . Tarwadi , . Churiyah , Pongtuluran Olivia Bunga , Juniarti Fifit , Wijaya Fery Azis 
Published: December 2017

Sambiloto (Andrographis paniculata) banyak digunakan untuk mengobati berbagai penyakit di Indonesia dan negara-negara Asia lainnya. Dalam studi ini, ekstrak metanol dan etanol sambiloto yang diperoleh dari B2PTO Tawangmangu telah diuji terhadap sel lini kanker payudara T47D dan MCF-7 dan sel lini normal fibroblast HFL-1 menggunakan reaksi enzimatik 3-(4,5-dimethylthiazoyl-2-yl) 2,5-diphenyltetrazoliumbromide (MTT). Uji in vitro terhadap sel lini normal fibroblast HFL-1 menunjukkan bahwa 50 ppm ekstrak metanol sambiloto tidak menghambat pertumbuhan sel. Tetapi, ekstrak metanol dan etanolnya menghasilkan IC50 yang relatif rendah pada sel lini kanker payudara, yaitu 111 ppm dan 122 ppm pada sel lini MCF-7 dan 70 ppm dan 197 ppm pada sel lini T47D. Selain itu, campuran ekstrak sambiloto yang mengandung 25% ekstrak Thyponium divaricatum dan Anredera cordifolia memberikan daya hambat pertumbuhan pada sel kanker payudara MCF-7 yang lebih besar, dengan nilai IC50 masing-masing adalah 68 ppm dan 34 ppm. Kesimpulannya, total ekstrak metanol atau etanol sambiloto yang diperoleh dari Tawangmangu memiliki potensi sebagai sumber senyawa anti-kanker serta perlu kajian lebih lanjut.Kata kunci: Ekstrak Andrographis paniculata, MTT, sel lini normal, sel lini kanker, aktivitas anti kanker ABSTRACTSambiloto (Andrographis paniculata) is widely used as medicine to treat various diseases in Indonesia and other Asian countries. In this study, methanolic and ethanolic extracts of sambiloto collected from B2PTO Tawangmangu have been tested againts breast cancer cell lines of T47D and MCF-7 and normal fibroblast cell line of HFL-1 using enzymatic reaction of 3-(4,5-dimethylthiazoyl-2-yl) 2,5-diphenyltetrazoliumbromide (MTT). In vitro assay performed on normal fibroblast of HFL-1 cell line showed that 50 ppm of methanolic extract of sambiloto did not inhibit cell growth. However, methanolic and ethanolic extracts of sambiloto gave relatively low of IC50 on breast cancer cell lines which were 111 ppm and 122 ppm on the MCF-7 cell lines and 70 ppm and 197 ppm on the T47D cell lines, respectively. In addition, the mixture of sambiloto extract containing 25% of Thyponium divaricatum and Anredera cordifolia extracts confered greater growth inhibition on breast cancer cell line of MCF-7, where IC50 values were 68 ppm and 34 ppm, respectively. In conclusion, the total methanolic or ethanolic extract of sambiloto collected from Tawangmangu has potency as a source of anti-cancer compounds and needs further study.Key words: Andrographis paniculata extract, MTT, normal cell line, cancer cell lines, anti-cancer activity
OPTIMALISASI MEDIA PRODUKSI AMILOGLUKOSIDASE MENGGUNAKAN FERMENTASI MEDIA PADAT
Authors: Sunaryanto Rofiq , Marasabessy Ahmad 
Published: December 2017

Amiloglukosidase merupakan salah satu enzim yang banyak digunakan dalam industri gula cair. Dalam proses fermentasi, komposisi medium fermentasi sangat berpengaruh terhadap aktivitas amiloglukosidase. Optimalisasi media padat proses produksi amiloglukosidase secara fermentasi padat dengan menggunakan isolat Aspergillus niger telah dilakukan. Optimalisasi media padat dilakukan dengan menentukan rasio dedak:pati terbaik yang selanjutnya dikombinasikan dengan beberapa sumber nitrogen. Dalam penelitian ini sumber nitrogen yang digunakan adalah sumber nitogen organik dan sumber nitrogen anorganik. Sumber nitrogen organik yang digunakan antara lain Corn Step Liquor dan pepton, adapun sumber nitrogen anorganik yang digunakan adalah amonium nitrat, amonium sulfat, dan amonium pospat. Fermentasi dilakukan selama 120 jam pada suhu 30ºC.Hasil percobaan menunjukkan bahwa kombinasi perbandingan media sumber karbon dedak:pati 1:1 menghasilkan produktivitas amiloglukosidase tertinggi 724 Unit/ml. Amonium sulfat merupakan sumber nitrogen anorganik terbaik dan mampu menghasilkan produktivitas amiloglukosidase sebesar 823 Unit/ml, sedangkan Corn Step Liquor (CSL) yang merupakan sumber nitrogen organik mampu menghasilkan produktivitas amiloglukosidase lebih tinggi dibandingkan pepton dengan produktivitas sebesar 884 Unit/ml.Keywords: Aspergillus niger, amyloglucosidase, fermentation, carbon source, nitrogen source ABSTRAKAmiloglukosidase adalah salah satu enzim yang banyak digunakan dalam industri gula cair. Dalam proses fermentasi, komposisi medium fermentasi sangat berpengaruh terhadap aktivitas amiloglukosidase. Pada penelitian ini telah dilakukan optimalisasi media padat pada proses produksi amiloglukosidase secara fermentasi padat dengan menggunakan isolat Aspergillus niger. Optimalisasi media padat dilakukan dengan menentukan rasio dedak:pati terbaik yang selanjutnya dikombinasikan dengan beberapa sumber nitrogen. Sumber nitrogen yang digunakan adalah sumber nitrogen organik dan anorganik. Sumber nitrogen organik yang digunakan antara lain adalah Corn Step Liquor (CSL) dan pepton, adapun sumber nitrogen anorganik yang digunakan adalah amonium nitrat, amonium sulfat, dan amonium fosfat. Fermentasi dilakukan selama 120 jam pada suhu 30ºC. Hasil percobaan menunjukkan bahwa kombinasi perbandingan media sumber karbon dedak:pati 1:1 menghasilkan produktivitas amiloglukosidase tertinggi, yaitu 724 unit/mL. Amonium sulfat merupakan sumber nitrogen anorganik terbaik dan mampu menghasilkan produktivitas amiloglukosidase sebesar 823 unit/mL, sedangkan CSL menghasilkan produktivitas amiloglukosidase lebih tinggi dibandingkan pepton, yakni sebesar 884 unit/mL.Kata kunci: Aspergillus niger, amiloglukosidase, fermentasi, sumber karbon, sumber nitrogen
INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (<i>Triticum aestivum</i> L.)
Authors: Sari Laela , Purwito Agus , Soepandi Didy , Purnamaningsih Ragapadmi , Sudarmonowati Enny 
Published: December 2017

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivum L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27–35°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27–35°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus
INDEKS LABILITAS UDARA UNTUK MEMPREDIKSI KEJADIAN BADAI GUNTUR PADA PERIODE PUNCAK MUSIM HUJAN TAHUN 2016
Authors: Zahroh Nyayu Fatimah , Dewi Ni Wayan Srimani Puspa , Harsanti Dini 
Published: December 2017

IntisariPrediksi kejadian badai guntur menjadi perhatian masyarakat luas karena biasanya disertai dengan curah hujan yang tinggi, terutama untuk wilayah rawan banjir seperti provinsi DKI Jakarta. Penelitian ini bertujuan untuk analisis potensi hujan disertai badai guntur dengan menggunakan data radiosonde, analisis indeks labilitas berdasarkan parameter yang didapat dari radiometer dan analisis tren indeks labilitas 6 jam sebelum kejadian hujan. Ada beberapa tanggal yang menjadi perhatian yaitu hari dimana kejadian hujan disertai badai guntur tinggi pada tanggal 28 - 31 Januari 2016 serta tanggal 14 Februari 2016 dan kejadian hujan tanpa badai guntur pada tanggal 3 - 4 Februari 2016. Hasil penelitian menunjukan bahwa terdapat perbedaan hasil sounding sebelum hujan dan pada saat hujan. Hasil sounding sebelum kejadian hujan memiliki labilitas moderat dan sounding pada saat kejadian hujan/badai guntur memiliki labilitas kuat. Selain itu, data dari radiometer menunjukan perbedaan signifikan antara perubahan tren indeks labilitas pada 6 jam sebelum kejadian hujan yang disertai badai guntur, dan pada kejadian hujan tanpa badai guntur. AbstractPredictions of a thunderstorm event become an attention for wide society because it is usually accompanied by heavy rainfall, especially for the flood prone area like the province of Jakarta. The objective of this study is to analyze the potential of rain with thunderstorms using radiosonde data, to analyze the lability indices based on parameters that obtained from Radiometer, and to analyze the trends of instability indices in 6 hours before the storm event. There are a few dates that become attention, the day where rain events with thunderstorms is high which is on January 28th - 31st, 2016 and February 14th, 2016, and the rain event without thunderstorm on February 3rd - 4th, 2016. The results showed that there are different sounding results before rain event and when it rains. The sounding result before rain event has moderate lability and sounding result when rain event has strong lability. In addition, the data from the radiometer showed a significant difference between the predicted 6 hours prior to the event of rain with a thunderstorm and rain without thunderstorm events.
Enzim Laccase dari Edible Mushroom untuk Pemutihan Pati Sagu Ramah Lingkungan
Authors: Reksohadiwinoto Budhi Santoso , Rosmalawati Syofi , Cahyana Purwa Tri , Hariyanto Bambang 
Published: December 2017

Sagu pada umumnya berwarna kecoklatan dan bau kurang sedap. Kelemahan ini dapat diperbaiki dengan penambahan enzim Laccase dalam produksi pati sagu. Tujuan penelitian ini adalah menghasilkan enzm Laccase dari edible mushroom. Bahan yang digunakan adalah jamur Plurotus ostreatus, Agaricus bisporus dan Rhyzopus oligosporus yang diperoleh dari pasar dan bahan kimia media. Metoda yang digunakan adalah melalui fermentasi cair menggunakan skala flask. Hasil yang diperoleh bahwa Pleurotus ostreatus pada uji agar cawan petri membentuk zona ring merah kecoklatan yang tebal menunjukan kemampuan oxidase senyawa guaicol dan syringaldazine. Uji fermentasi cair skala flaks 500 mL kultur jamur Pleurotus ostreatus, Agaricus bisporus, Rhyzopus oligosporus selama 21 hari. Hasil Laccase yang diproduksi oleh Pleurotus ostreatus mempunyai aktivitas tertinggi yaitu mencapai 774 U/L pada analisa spektrofotometer menggunakan substrat ABTS. Sementara itu, Agaricus bisporus dan Rhyzopus oligosporus mencapai puncak produksi laccase masing-masing 484 U/L dan 480 U/L.Kata kunci : Pleurotus ostreatus, Agaricus bisporus, Rhyzopus oligosporus, Laccase, bioleaching, dan kualitas pati sagu
PERANCANGAN JIG MD CUTTING SEBAGAI PENGGANTI PROSES PEMOTONGAN MANUAL PADA MD KONEKTOR
Authors: Maulana Febryan 
Published: December 2017

Pada industri pembuatan konektor, produsen dituntut agar menghasilkanÂ produk yang murah dengan tidak mengurangi kualitas produk yangÂ dihasilkan. Hal ini menuntut perusahaan melakukan peningkatan kapasitasÂ produksi tanpa Â mengurangi kualitas produk yang dihasilkan, serta tanpaÂ mengabaikan faktor keselamatan kerja operator. Untuk dapat memenuhiÂ semua tuntutan itu maka diperlukan sebuah alat yang dapat menghasilkanproduk dengan kualitas yang baik, dan seragam serta waktu produksi yangÂ efisien.Jig MD cutting dirancang guna memenuhi kebutuhan produksi akanÂ alat potong batang produk MD konektor. Yang pada awalnya prosesÂ pemotongan dilakukan dengan bantuan gunting kuku, akan tetapiÂ pemotongan dengan gunting kuku hasilnya kurang baik, prosesnya memakanÂ waktu yang cukup lama dan faktor keamanan nya kurang. Dari hasilÂ pengujian, alat ini dan metode pemotongan sebelumnya. Dapat disimpulkanÂ bahwa alat ini mampu menghasilkan produk dengan kualitas pemotonganÂ yang lebih akurat dengan tingkat keseragaman yang merata, dan prosesÂ pemotongan yang lebih cepat.
Canadian Health Libraries’ Responses to the Truth and Reconciliation Commission’s Calls to Action: A Literature Review and Content Analysis
Authors: Maestro Lara , Chadwick Daniel James 
Published: December 2017

Abstract Introduction: As part of the Truth and Reconciliation Commission of Canada’s (TRC) Final Report on the history and legacy of residential schools in Canada, ninety-four (94) Calls to Action were identified. Of those, seven are health-specific. The objective of this research paper is to determine how Canadian health library websites are responding to these calls to action. Methods: The authors conducted an initial literature review to gain an understanding of the context of Indigenous health in Canada. A content analysis of Canadian health library websites was conducted to track mentions of the TRC and their responses to the need for Indigenous-focused resources. Results: The results of content analysis indicated few online responses to the TRC’s Calls to Action from Canadian health libraries. Only thirty-three per cent of Canadian health libraries had content that was Indigenous-focused, and only about fifteen per cent of health libraries had visible content related to the TRC’s Calls to Action. Academic and consumer health libraries were more likely to have both TRC- and Indigenous-focused content. Discussion: Nuances related to the research question resulted in some challenges to research design. For example, website content analysis is an imperfect indicator of real-world action. Limitations in research design notwithstanding, visibility is an important part of conveying commitment to the TRC, and the information available indicates the Canadian medical community is not living up to that commitment. Conclusion: Canadian health libraries need to do more to show a visible commitment to the TRC’s Calls to Action.
Esporte e idosos: jogar, competir e viver
Authors: Simões Regina , Castellano Simone Maria 
Published: December 2017

ResumoO objetivo é relacionar o fenômeno esportivo com o idoso a partir das possibilidades de conceitos e da vivência em esportes. A coleta de dados foi realizada com 37 idosos, integrantes de uma equipe representativa de uma cidade do interior de São Paulo nos Jogos Regionais do Idoso (JORI), os quais responderam a um questionário. A análise dos dados foi através da Técnica de Elaboração e Análise de Unidades de Significado. Os dados revelaram que os idosos estão na equipe num período entre um e oito anos e as razões de participar estão atreladas: à amizade e ao companheirismo, à saúde, ao preparo físico, a sentir-se bem, gostar da prática de esportes, trocar experiências, conhecer novas cidades. Estes resultados mostram a necessidade de a Educação Física proporcionar, na forma adequada, a prática de esportes para essa faixa etária.Palavras-chave: Esporte. Idoso. Jogo.Sport and elderly: to play, to compete and to liveAbstractThe aim is to relation the sporting phenomenon with elderly, at the beginning of the possibilities of the concepts and the sport experiencing. We applied a questionnaire in 37 elderly, part of the representative team of the country city of São Paulo state in the Regional Games of elderly people (JORI). The questionnaires were transcribed and responses were analyzed through content analysis: Technical Preparation and Analysis of Meaning Units. The data reveals that elderly are in the team in the period between one and eight years and the reasons to participate are attached to: friendship, health, physics prepare, to feel better and to like the practices of sports, to switch experience or to know new cities. This result appoint to the needs of the Physics Education to provide, in the corrected way, the practice of sports to these people included.Keywords: Sport. Elderly. Game.Deporte y ancianos: jugar, competir y vivir ResumenEl objetivo es relacionar el fenómeno deportivo con el anciano a partir de las posibilidades de conceptos y de la vivencia en deportes. La recolección de datos fue realizada con 37 ancianos, integrantes de un equipo representativo de una ciudad del interior de São Paulo en los Juegos Regionales del Anciano (JORI), los cuales respondieron a un cuestionario. El análisis de los datos fue a través de la Técnica de Elaboración y Análisis de Unidades de Significado. Los datos revelaron que los ancianos están en el equipo en un período entre uno y ocho años y las razones de participar están enganchadas: a la amistad y al compañerismo, a la salud, a la preparación física, a sentirse bien, gustar la práctica de deportes, cambiar experiencias, conocer nuevas ciudades. Estos resultados muestran la necesidad de que la Educación Física proporcione, en la forma adecuada, la práctica de deportes para este grupo de edad.Palabras clave: Deporte. Anciano. Juego.
SAVE THE BIO: O DESIGN COMO FERRAMENTA CONTRA A PERDA DA BIODIVERSIDADE
Authors: Chiaradia Liana Domeneghini , Pazmino Ana Veronica 
Published: December 2017

A sociedade atual vive em um sistema linear em crise, baseado no consumo. Isto causa uma série de problemas ambientais e sociais, entre eles a perda da biodiversidade, que compromete toda a vida no planeta Terra. Neste artigo são abordados dados que comprovam a gravidade deste fato e alternativas para reduzi-lo, por meio do Design. São realizadas análises de cases de Design Gráfico e de Design de Interação, e uma proposta de uma nova ferramenta: o aplicativo Save the Bio. ABSTRACT The current society lives in a linear system in crisis, based on consumerism. Its causes a lot of environmental and social problems, and one of them is the loss of biodiversity, that affects all the life on Earth. In this article are discussed details that prove the seriousness of this fact and alternatives to reduce it, through Design. Graphic Design and Interaction Design cases are analyzed, and a new tool is proposed: Save the Bio app.
AVALIAÇÃO ECONÔMICA E DE EMISSÕES DE CO2 DA RECICLAGEM DE RESÍDUOS DE CONSTRUÇÃO E DEMOLIÇÃO CLASSE A: ESTUDO DE CASO PARA GOIÂNIA - GO
Authors: Sacho Sara Duarte , Caldas Lucas Rosse , Pfeiffer Simone Costa , Sposto Rosa Maria 
Published: December 2017

O uso cada vez maior de resíduos sólidos da construção e demolição (RSCD), principalmente os resíduos Classe A, para a produção de agregados para pavimentação surgiu nos últimos anos como uma alternativa interessante para atingir os objetivos da sustentabilidade ambiental. No entanto, o seu custo muitas vezes ainda é elevado devido entre outros fatores, ao desenvolvimento tecnológico nacional existente para reciclagem, incluindo o uso de energia. Este trabalho trata da análise da viabilidade econômica e ambiental destes resíduos. Além dos custos, foi feita uma quantificação das emissões de dióxido de carbono (CO2) da reciclagem de resíduos Classe A, considerando-se um estudo de caso no município de Goiânia - GO. Foram coletados dados em uma usina de reciclagem existente na região. A partir da análise econômica, observou-se que o material reciclado apresentou valor de mercado superior ao do agregado convencional fornecido pelas principais empresas goianas. Em relação às emissões de CO2, observou-se que a usina de reciclagem gera 2,6 kgCO2/t, valor próximo aos encontrados na literatura. Concluiu-se que a reciclagem de resíduos Classe A para a realidade goiana é viável do ponto de vista de emissões, no entanto não vantajosa do ponto de vista econômico.
BAMBU – O AÇO VEGETAL
Authors: Carbonari Gilberto , Silva Junior Nelson , Pedrosa Nicolas Henrique , Abe Camila Hiromi , Scholtz Marcos Ferreira , Acosta Caio Cesar Veloso , Carbonari Luana Toralles 
Published: December 2017

Com os problemas gerados pelos materiais mais utilizados na construção civil, como o consumo de energia, a poluição e a inviabilidade econômica, surge a necessidade de alternativas que unam o conceito de fonte renovável ao de sustentabilidade. Nesse sentido, o bambu se mostra uma importante opção, considerando seu crescimento rápido e sua alta produção. O estudo de suas características mecânicas é fundamental para o projeto de construções em bambu. Com este objetivo, a metodologia experimental utilizada permite a determinação de alguns destes parâmetros, tais como, as resistências à compressão, à tração, à flexão, e o módulo de elasticidade longitudinal. Os testes foram realizados em 9 espécies localizados no IAPAR/Londrina, oriundas da China. Considerando as variações geométricas decorrentes de alterações naturais nos bambus, os resultados confirmaram a eficácia e confiabilidade nos métodos de ensaio usados. A partir destes resultados pode-se afirmar que o bambu apresenta elevadas propriedades mecânicas. Dividindo as resistências à tração e à compressão pela densidade de cada material, todas as espécies estudadas se mostram mais eficientes que o concreto e o aço, conferindo ao bambu a designação de “aço vegetal”. Além disso, o bambu apresenta um potencial construtivo ecologicamente menos agressivo que os materiais construtivos tradicionais e uma considerável leveza.
Modification of chlorine dioxide bleaching of Gmelina arborea (gamar) pulp
Authors: Jahan M Sarwar , Uddin MM , Kashem MA 
Published: December 2017

Elemental Chlorine Free (ECF) pulp bleaching is now the dominant pulp bleaching process in globally. In most bleachery, chlorine dioxide is over-consumed. About two thirds of the chlorine dioxide is wasted in useless side reactions. In the study, kraft pulp from Gmelina arborea (gamar wood) was bleached by ECF bleaching in modified sequences. Oxygen prebleaching was carried out to decrease ClO2 requirement, which reduced kappa number of kraft pulp by 47.6% and increased pulp brightness by 21.7 percent points. Several sequences were tested based on the application of limited charges of ClO2 during successive ClO2and extraction stage. Application of this concept allowed a 33% reduction of ClO2 to reach target brightness. The kraft pulp could not reach target brightness of 80% in DED sequences using even 30 kg ClO2/ton of pulp, while splitting of same amount of ClO2 charge into DEDED sequences reached the pulp brightness to 81.1%. But oxygen delignified kaft pulp reached 79.6% brightness using 25 kg ClO2/ton of pulp in DED sequences. In the splitting of ClO2 charge into DEDED sequences, Oxygen pulp reached to 85% brightness by using only 20 kg ClO2/ton pulp.Bangladesh J. Sci. Ind. Res. 52(4), 247-252, 2017