bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of Inflammatory Central Nervous System Diseases After Tumor Necrosis Factor–Inhibitor Treatment for Autoimmune Diseases
Authors: Xie Wenhui , Sun Yunchuang , Zhang Wei , Zhu Nanbo , Xiao Shiyu 
Published: October 2024

ImportanceTumor necrosis factor (TNF) inhibitors have been used extensively to treat various autoimmune diseases. However, there are ongoing debates about the risk of inflammatory central nervous system (CNS) disease events following TNF inhibitor therapy, as well as uncertainty about how this risk varies across different autoimmune diseases or TNF-blocking agents.ObjectiveTo evaluate the risk of inflammatory CNS diseases after anti-TNF initiation and assess the difference in risk among different types of underlying autoimmune diseases or TNF inhibitors.Data SourcesSeparate searches were conducted across PubMed, Embase, and the Cochrane Library from inception until March 1, 2024.Study SelectionObservational studies assessing the association between anti-TNF therapy and inflammatory CNS diseases relative to a comparator group.Data Extraction and SynthesisStudy eligibility assessment and data extraction were independently conducted by 2 investigators following PRISMA guidelines. The risk ratio (RR) was used as the effect measure of the pooled analysis.Main Outcomes and MeasuresThe primary outcome was the risk of incident inflammatory CNS events after anti-TNF therapy for autoimmune diseases. Secondary analyses were performed based on different types of underlying autoimmune diseases and TNF inhibitors.ResultsEighteen studies involving 1 118 428 patients with autoimmune diseases contributing more than 5 698 532 person-years of follow-up were analyzed. The incidence rates of new-onset inflammatory CNS events after initiating TNF inhibitors ranged from 2.0 to 13.4 per 10 000 person-years. Overall, exposure to TNF inhibitors was associated with a 36% increased risk of any inflammatory CNS disease compared to conventional therapies (RR, 1.36; 95% CI, 1.01-1.84; I2, 49%), mainly attributed to demyelinating diseases (RR, 1.38; 95% CI, 1.04-1.81; I2, 31%). Secondary analyses revealed a similar risk of inflammatory CNS diseases across different types of underlying autoimmune diseases (rheumatic diseases: RR, 1.36; 95% CI, 0.84-2.21; inflammatory bowel disease 1.49; 95% CI, 0.93-2.40; P for subgroup = .74) and TNF inhibitors (anti-TNF monoclonal antibodies vs etanercept: RR, 1.04; 95% CI, 0.93-1.15; I2, 0%).Conclusions and RelevanceCompared to conventional therapies, exposure to TNF inhibitors was associated with a 36% increased risk of inflammatory CNS diseases, irrespective of background autoimmune disease or TNF inhibitor type.
Platycodin D reduces PD‐L1 levels by inhibiting LXR‐β activity and combines with nintedanib to enhance the tumor‐killing effect of T cells
Authors: Lei Jin , Cao Xue‐Wei , Li Peng‐Fei , Zhao Jian , Wang Fu‐Jun 
Published: October 2024

Most tumors are resistant to programmed cell death protein 1 (PD‐1)/programmed death‐ligand 1 (PD‐L1) checkpoint inhibitors, which may be due to impaired antigen presentation resulting from the downregulation of major histocompatibility complex class I (MHC‐I) expression on tumor cells. We observed that platycodin D (PD), polygalacin D, and platycodin D2, which are plant‐derived triterpenoid saponins, significantly reduced PD‐L1 levels. RNA sequencing and the PharmMapper database analysis identified liver X receptor β (LXR‐β) as a potential PD target. Further studies showed that PD reduces PD‐L1 levels by binding to LXR‐β and inhibiting LXR‐β activity. Coadministration of PD and nintedanib, known to upregulate MHC‐I expression, enhanced tumor recognition and killing by T cells. This study provides new insights into PD applications and mechanisms.
An Update on Theoretical and Metrological Aspects of the Surface Hydrophobicity of Virus and Virus‐Like Particles
Authors: Sautrey Guillaume 
Published: October 2024

AbstractViruses are biological entities embodied in protein‐based nanoparticles devoid of metabolic activity. Hence, the colloidal, interfacial, and chemical reactivity of virus particles (VPs) profoundly affects the fate of natural and artificial viruses in biotic or abiotic aqueous systems. These rely on the physical chemistry at the outer surface of VPs. In other words, whether wild or synthetic VPs and regardless of the scientific fields involved, taming viruses implies thus managing the physical chemistry at the VP external surface. The surface hydrophobicity (SH) of VPs is a critical feature that must be looked at. Still, the literature dealing with nanoscale hydrophobic domains at the proteinaceous surface of VPs underlying their global SH is like a fragmented puzzle. This article provides an overview of the topic from the perspective of modern protein biophysics for updating the classic physicochemical picture of outer VP/water interfaces hitherto accepted. Patterns of non‐polar and “false‐polar” patches, expressing variable hydrophobic degrees according to neighboring polar patches, are now drawn. The extensive discussion of reviewed data generates such fresh ideas to explore in the coming years for better modeling the SH of wild virions or engineered virus‐based nanoparticles, paving the way for new directions in fundamental virology and virus‐based chemistry.
High Safety Fuel Cells Based on Gas Diffusion Layer Suppressed Uneven Current and Thermal Runaway
Authors: Ning Fandi , Zhu Xingyu , Liu Yiyang , Hammad Ali , Huang Jiahao , Chai Zhi , Shen Min , He Can , Wen Qinglin , Dan Xiong , Li Wei , Tian Bin , He Lei , Zhou Xiaochun 
Published: October 2024

AbstractThermal runaway poses a critical safety concern for fuel cells, significantly impairing their performance and durability. Uneven current often leads to uneven temperature, exacerbating the risk of thermal runaway. The inadequate electrical and thermal conductivity of the gas diffusion layer (GDL) is identified as the primary cause of the safety issues. To address this, a multilayer composite gas diffusion layer (MC‐GDL) is proposed and designed to enhance both electrical and thermal conductivity. The in‐plane electrical conductivity of the MC‐GDL reaches an impressive 9.1 × 104 S m−1. Under extreme uneven current, the fuel cell's performance with MC‐GDL only declines by 3.7%, compared to a substantial 58.1% decline observed with commercial GDL. Additionally, the in‐plane thermal conductivity of the MC‐GDL is notably high at 337.0 W m−1 K−1. Under extreme uneven temperature, the maximum temperature of Nafion membrane in fuel cell equipped with MC‐GDL is only 84.2 °C significantly lower than the 180.7 °C observed with commercial GDL. Consequently, the MC‐GDL effectively prevents the melting, thinning, and perforation of the Nafion membrane, thereby mitigating thermal runaway. Furthermore, the superior electrical and thermal conductivity of MC‐GDL can enhance the safety of other electrochemical devices by minimizing uneven current and thermal runaway.
Plasma‐Enhanced Magnetron Sputtering: A Novel Approach for Biofunctional Metal Nanoparticle Coatings on Reverse Osmosis Composite Membranes
Authors: Michler Nicole , Hirsch Ulrike M. , Steinert Carolin , Fritzsche Gregor , Schmelzer Christian E. H. 
Published: October 2024

AbstractReverse osmosis (RO) is the most common method for treating salt and brackish water. As a membrane‐driven process, a key challenge for RO systems is their susceptibility to scaling and biofouling. To address these issues, functional coatings utilizing metal nanoparticles (MNPs) are developed. In this study, silver, gold, and copper nanoparticles are applied onto thin‐film composite (TFC) membranes using plasma‐enhanced magnetron sputtering. The elemental composition, surface morphology, and hydrophilicity of the coatings are analyzed using X‐ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and contact angle measurements. The antimicrobial properties and the filtration efficiency of the coated membranes are assessed through application‐specific experimental setups. Silver and copper nanoparticles exhibit superior antimicrobial properties, reducing microorganism adhesion by a factor of 103 compared to uncoated membranes. Under appropriate coating conditions, no deterioration in filtration performance is observed. Enhancing the adhesion of MNPs is necessary for achieving sustained release of metal ions.
Control of Orientation and Periodicity of Laser‐Induced Surface Structures on Metals
Authors: Shvedov Vladlen G. , Izdebskaya Yana V. , Shadrivov Ilya V. 
Published: October 2024

AbstractLaser‐induced periodic surface structures (LIPSS) can be created on various materials, and they hold an exceptional potential for surface nanopatterning, enabling new industrial applications in medicine, biology, optics and other fields. However, LIPSS formation is typically restricted to a specific orientation and periodicity. In this work, a novel approach is demonstrated for full control of the LIPSS periodicity and orientation on metallic surfaces using a 1064 nm nanosecond laser. Analytical expressions and experimental verification are presented to show that by simultaneously manipulating three parameters of the laser beam, such as the polarization, angle of incidence, and direction of the laser scan along the surface, LIPSS can be formed with the desired geometrical configuration. This enhanced control opens vast possibilities for laser processing technologies as a flexible and highly competitive solution for advanced applications relying on surface modifications in the fields of anisotropic surface wettability, thermal and electrical conductivity, structured colors, diffraction gratings, and many others.
Full‐Color Tunability Room Temperature Phosphorescence from Inorganic Crystal Frameworks
Authors: Huang Yunze , Zhang Weibo , Yan Siyu , Xin Hongpu , Li Peng 
Published: October 2024

AbstractRoom temperature afterglow (RTA) materials have aroused prodigious research interests owing to their unique long‐life luminescent properties. However, it is rare for RTA materials to be reported with full‐color afterglow emission. Herein, a universal strategy is designed to activate full‐color tunability RTA based on phenylboronic acid derivatives (BOH) and boric acid (BA) via an ingenious solvent‐free solid‐phase heating. As the conjugation degrees of aromatic groups of the guest molecule expanded, the BOH/BA composites showed tunable afterglow colors ranging from blue to red after ultraviolet (UV) irradiation. This strategy is to firmly anchor the guest molecule in a rigid framework of the inorganic metaboric acid matrix by abundant hydrogen‐bonding interactions between the host and guest, suppressing molecular motion and promoting the intersystem crossing (ISC) rate between the singlet and triplet excited states for enhanced afterglow emission. In addition, through mixing in multiple guest molecules into BA simultaneously, the obtained composite can be modulated in a wide excitation range from blue to red. This fascinating study provides a simple and universal method to fabricate full‐color tunability RTA composites, making them a promising candidate for applications in advanced information security and multi‐level encryption.
Ruthenium(II)‐Catalyzed Pyridyl‐Directed Tryptophan C−H Acylmethylation with α‐Chloro Ketones
Authors: Hu Shulei , Wang Yong , Xie Xiong , Liang Renwen , Liu Hong , Wang Jiang 
Published: October 2024

AbstractA ruthenium(II)‐catalyzed C−H acylmethylation of Trp‐containing peptides with α‐chloro ketones is reported here. This reaction features good C‐2 selectivity and chemoselectivity, making it suitable for late‐stage modification of Trp‐containing peptides. Low‐cost metal ruthenium as a catalyst enables the reaction to be conducted on a gram scale. This report also discusses the synthetic applications and presents a method to remove the pyridine directing group. In addition, a plausible mechanism of the C(2)−H acylmethylation reaction is proposed in this article.
Investigation of AMA Synthase from Pyrenophora teres f. teres 0–1 for the Synthesis of Aspergillomarasmine A Analogues and Non‐Canonical Amino Acids
Authors: Zhu Zhixi , Ma Yue , Xie Xiaoying , Wang Zhiyuan , Han Lu , Song Jingwu , Liang Yuzong , Poelarends Gerrit J. , Chen Yijun , Lu Meiling , Zhang Jielin 
Published: October 2024

AbstractThe fungal natural product aspergillomarasmine A (AMA) is an inhibitor of metallo‐β‐lactamases and a promising co‐drug candidate for reversing bacterial β‐lactam resistance. The biosynthesis of AMA from L‐Asp and O‐phospho‐L‐serine is catalyzed by AMA synthase, which possesses high synthetic potential for the biocatalytic preparation of AMA and related aminopolycarboxylic acids. Here, we identified and structurally characterized an AMA synthase from Pyrenophora teres f. teres 0–1 (PteAMAS). Crystallographic analysis revealed a unique homotetrameric fold and a highly hydrophilic active site, which supports the PLP‐dependent β‐replacement catalytic mechanism. Furthermore, we demonstrated the utility of PteAMAS for the biocatalytic synthesis of AMA and its analogues, achieving structural diversification of the APA1 moiety through a modular two‐enzyme system involving PteAMAS and a selective C−N lyase, namely EDDS lyase or 3‐methylaspartate ammonia lyase variant MAL−Q73 A. In addition, we exhibited the usefulness of PteAMAS for convenient synthesis of various S‐alkyl, ‐aryl, and ‐arylalkyl substituted L‐cysteines, which are valuable non‐canonical amino acids with broad pharmaceutical applications. The present study has not only provided the structural basis for catalysis and substrate recognition by PteAMAS, but also paved the way for biocatalytic preparation of structurally complex AMA analogues and various non‐canonical amino acids.
Chronic Exposure to Bioaerosols in PM2.5 from Garbage Stations Accelerates Vascular Aging via the NF‐κB/NLRP3 Pathway
Authors: Chen Peier , Ning Xiaodong , Feng Weijing , Li Yajing , Chen Guoqin , Shi Xu , Pan YuXuan , Shi Xueqin , Xiao Yafang , Liu Yuhua , Zhang Guoxia , Zhou Feiran , Ou Caiwen 
Published: October 2024

AbstractThe fine particulate matter (PM2.5) in air pollution is a critical risk factor influencing human health. Our study included 8144 participants and showed that the risk of major adverse cardiovascular events increases by 35% (HR, 1.35; 95% CI, 1.14–1.60) for participants with the highest quartile to PM2.5 exposure as compared to those with lowest quartile. Bioaerosols, as an important environmental exposure in PM2.5, can induce systemic chronic inflammation leading to vascular aging. Thus, the effects of bioaerosols are investigated from household garbage stations in PM2.5 on vascular aging, and the underlying mechanisms are explored. In vivo, chronic exposure to bioaerosols upregulated senescence marker expression levels while causing vascular dysfunction and remodeling. In vitro, bioaerosol exposure induced decreased proliferation, G0/G1 arrest, and impaired migration of human umbilical vein endothelial cells (HUVECs). Furthermore, a single bacterium (AS22a) from the bioaerosol community was isolated and demonstrated that it upregulated inflammatory factors and accelerated cell senescence and vascular aging by activating the NF‐κB/NLRP3 signaling pathway, which may serve as a primary mechanism underlying vascular aging induced by bioaerosols in PM2.5. These findings suggest that high levels of bioaerosols in household garbage stations may adversely affect cardiovascular health.
Parkinson Disease ‐Targeted Nanocapsules for Synergistic Treatment: Combining Dopamine Replacement and Neuroinflammation Mitigation
Authors: Liu Ziyao , Xiang Shijun , Chen Bei , Li Jian , Zhu Dingcheng , Xu Hongjuan , Hu Shuo 
Published: October 2024

AbstractParkinson's disease (PD) is characterized by dopamine (DA) neuron loss and neuroinflammation. This study develops carrier‐free nanocapsules (NCs) for targeted delivery of DA and catalase (CAT) to the PD brain, addressing both DA depletion and neuroinflammation simultaneously. The NCs are engineered by DA and 4‐formylphenylboronic acid co‐loading with cRGD‐modified CAT (CAT‐cRGD) and surface‐modifying with Angiopep‐2 (Ang). Ang targets the blood‐brain barrier (BBB), enhancing brain delivery, while cRGD targets upregulated integrin receptors in the PD‐affected BBB. The NCs showed a 1.4‐fold increase in parkinsonian brain targeting efficiency compared to normal mice. In PD mice models, NCs demonstrated a stable increase in learning and memory, enhanced locomotor activity, and improved motor coordination. DA supplementation significantly enhanced dopaminergic signaling, increasing DA levels 1.8‐ and 3.5‐fold in the striatum and substantia nigra, respectively. Additionally, delivered CAT effectively reduced neuroinflammation by mitigating endoplasmic reticulum stress, slowing disease progression, and protecting DA from oxidation. This innovative approach using PD‐targeted NCs represents a synergistic strategy for PD treatment, combining symptomatic relief with disease progression intervention.
A Multi‐Responsive Hydrogel Combined With Mild Heat Stimulation Promotes Diabetic Wound Healing by Regulating Inflammatory and Enhancing Angiogenesis
Authors: Dai Fanjia , Zhang Jiaying , Chen Fengjiao , Chen Xianwu , Lee Celine Jessica , Liang Hongze , Zhao Lingling , Tan Hui 
Published: October 2024

AbstractThe repair of diabetic wound still encounters huge challenges, such as disordered inflammatory regulation and impaired neovascularization. Here, a pH/ROS/glucose responsive and photothermal hydrogel is developed for diabetic wound healing. The hydrogel is formed through cross‐linkage between phenylboronic acid‐modified carboxymethyl chitosan (CMCS‐PBA) and oxide dextran (OXD), utilizing Schiff base and phenylboronate ester bonds. Additionally, insulin‐like growth factor 1 C domain (IGF‐1C) and deferoxamine‐loaded polydopamine nanoparticles (D@P) are incorporated into the hydrogel. The hydrogel demonstrates sustained drug release, excellent photo thermal effect, prominent antioxidant, antibacterial and anti‐inflammatory activities, desirable mechanical and tissue adhesive properties, enhanced tube formation, and cell migration. Furthermore, the hydrogel combined with mild heat treatment can regulate chronic inflammation by promoting the transformation of macrophages from M1 phenotype to M2 phenotype and enhance angiogenesis by up‐regulating the expression levels of angiogenesis‐related factors such as hypoxia‐inducible factor‐1 alpha (HIF‐1α), vascular endothelial growth factor (VEGF), CD31, and α‐SMA, thus greatly accelerates the wound healing in streptozotocin (STZ)‐induced diabetic mice. Therefore, this multi‐responsive and multifunctional hydrogel holds potential as a therapeutic strategy for diabetic wounds.
Bionic Fluorine‐Free Multifunctional Photothermal Surface for Anti/de/Driving‐Icing and Droplet Manipulation
Authors: Wang Lin , Zhang Chengchun , Zhang Yasong , Shen Chun , Xin Zhentao , Wei Zhenjiang 
Published: October 2024

AbstractDue to safety concerns associated with ice accumulation, there is a need to develop a durable surface with anti/de‐icing properties. Inspired by Gecko skin and Nepenthes, a superhydrophobic photothermal surface (SH‐PS) with micro‐nano hierarchical structure based on carbon nanotube composites is prepared by one‐step laser method. It can be switchable to a slippery photothermal surface (S‐PS) by injecting silicone oil. S‐PS can effectively delay icing at −20 °C/−30 °C, and maintain excellent dynamic anti‐icing capabilities following 28 times supercooled droplet impact (−30 °C). Under near‐infrared (NIR) irradiation, S‐PS exhibits high‐efficiency photothermal deicing and excellent ice droplet control capabilities. Furthermore, S‐PS also exhibits remarkable droplet manipulation capabilities under NIR irradiation, even anti‐gravity transport (8°) and programmable track manipulation. Notably, after 20 m abrasion, the S‐PS still maintains good performances of anti/de‐icing and droplet manipulation after infusing lubricant. All these excellent performances can promote its application in a wider range of fields.
Designing High‐Sensitivity Mechanochromic Luminescent Materials Through Friction‐Induced Crystallization Strategy
Authors: An Zhihang , Dai Zhenhao , Liu Jiaping , Chen Si , Wang Xu , Liu Heyang , Sheng Zhongyi , Shan Tianyu 
Published: October 2024

AbstractDespite recent significant breakthroughs in novel mechanochromic luminescent (ML) materials, developing a high‐sensitivity ML material is still challengeable. Herein, a “friction‐induced crystallization” strategy is proposed to realize highly sensitive transformations of luminescent signal, through an integration of polymeric chains and an aggregation‐sensitive luminescent core, which act as mechanical sensors and fluochromic actuators, respectively. The coupling of these two components enables the material to crystallize in response to shear friction, thereby exhibiting blue‐shift fluorescence due to a more restricted relaxation pathway. This study underscores a high‐sensitivity ML material based on the precise regulation of molecular‐scale motions, and also expands the scope and potential of ML materials toward user‐friendly, interactive wearable devices.
Structures of a DNA Polymerase Caught while Incorporating Responsive Dual‐Functional Nucleotide Probes
Authors: Ghosh Pulak , Betz Karin , Gutfreund Cédric , Pal Arindam , Marx Andreas , Srivatsan Seergazhi G. 
Published: October 2024

AbstractFunctionalizing nucleic acids using DNA polymerases is essential in biophysical and biotechnology applications. This study focuses on understanding how DNA polymerases recognize and incorporate nucleotides with diverse chemical modifications, aiming to develop advanced nucleotide probes. We present the crystal structures of ternary complexes of Thermus aquaticus DNA polymerase (KlenTaq) with C5‐heterocycle‐modified environment‐sensitive 2′‐deoxyuridine‐5′‐triphosphate (dUTP) probes. These nucleotides include SedUTP, BFdUTP and FBFdUTP, which bear selenophene, benzofuran and fluorobenzofuran, respectively, at the C5 position of uracil, and exhibit high conformational sensitivity. SedUTP and FBFdUTP serve as dual‐app probes, combining a fluorophore with X‐ray anomalous scattering Se or 19F NMR labels. Our study reveals that the size of the heterocycle influences how DNA polymerase families A and B incorporate these modified nucleotides during single nucleotide incorporation and primer extension reactions. Remarkably, the responsiveness of FBFdUTP enabled real‐time monitoring of the binary complex formation and polymerase activity through fluorescence and 19F NMR spectroscopy. Comparative analysis of incorporation profiles, fluorescence, 19F NMR data, and crystal structures of ternary complexes highlights the plasticity of the enzyme. Key insight is provided into the role of gatekeeper amino acids (Arg660 and Arg587) in accommodating and processing these modified substrates, offering a structural basis for next‐generation nucleotide probe development.