bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural Palm Olein Polyol as a Replacement for Polyether Polyols in Viscoelastic Polyurethane Foam
Authors: Nurul ‘Ain H. , Maznee T. I. Tuan Noor , Norhayati M. N. , Noor M. A. Mohd , Adnan S. , Devi P. P. Kosheela , Norhisham S. Mohd , Yeong S. K. , Hazimah A. H. , Campara Irma , Sendijarevic Vahid , Sendijarevic Ibrahim 
Published: April 2016

AbstractThe impact of replacing three polyether polyols with different levels of a single palm olein‐based natural oil polyol (NOP) was systematically correlated with the changes in foaming reactivity, cell structure, physico‐mechanical properties, and morphology of viscoelastic (VE) foams. The data show that replacing the polyether polyols with the NOP slightly increased the rate of the foaming reactivity. Increasing the NOP content resulted in increased cell size and cells remained fully open. Increased NOP content contributed to higher load bearing properties of VE foam, which can be attributed to higher functionality of NOP compared to polyether polyols. Addition of the NOP slightly increased the resilience of the foams, however, the hysteresis which is the measure of energy absorption remained mostly unaffected. Age properties, characterized by dry and humid compression sets, were mostly unaffected by the replacement of the polyether polyol with the NOP. The addition of NOP did not impact the morphology of the VE foam polymer matrix, which appears to retain a low degree of hard and soft segment domain separation. Overall, the results demonstrate a feasibility that the NOP can be used to partially replace the polyether polyols in VE polyurethane foams without significant impact on the functional performance.
To end life or to save life: ageism moderates the effect of message framing on attitudes towards older adults’ suicide
Authors: Gamliel Eyal , Levi-Belz Yossi 
Published: April 2016

ABSTRACTBackground:Global suicide rates among older adults are very high. Public attitudes towards older adults’ suicide may affect older adults upon their contemplating such an act. Previous research has demonstrated that message framing affects persons’ judgments and decision making. Thus, message framing may have particular significance in the context of attitudes towards end-of-life phenomena, such as physician-aided suicide. This study examined the possible role of ageism in moderating the effect of message framing on attitudes towards older adults’ suicide.Methods:Two studies examined the association between ageism and attitudes towards older adults’ suicide. Study 1 assessed both variables by self-administered questionnaires; Study 2 further examined these variables, incorporating participants’ responses to a suicide-related vignette, and evaluating the possible effect of message framing, using a between-participants design.Results:High-ageism participants expressed greater acceptance for older adults’ suicide, whereas low-ageism participants expressed a less permissive approach to it (Study 1). In addition, ageism moderated the effect of message framing on attitudes towards older adults’ suicide: High-ageism participants revealed a more permissive attitude towards older adults’ suicide when the issue was presented in positive terms ofnot prolonging life, relative to a negative presentation ofending life; a similar effect was not found for low-ageism participants (Study 2).Conclusions:The moderating effect of ageism on attitudes towards older adults’ suicide has both theoretical and practical implications. We discuss these implications with respect to suicide prevention among older adults, and suggest future research.
MicroRNA-92b promotes hepatocellular carcinoma progression by targeting Smad7 and is mediated by long non-coding RNA XIST
Authors: Zhuang L K , Yang Y T , Ma X , Han B , Wang Z S , Zhao Q Y , Wu L Q , Qu Z Q 
Published: April 2016

AbstractMicroRNA (miRNA) and long non-coding RNA (lncRNA) have been demonstrated to participate in the progression of many cancers. Hepatocellular carcinoma (HCC) is one of the most common and aggressive malignant tumors worldwide, while the molecular mechanisms underlying HCC tumorigenesis are not completely clear. In this study, we showed that miR-92b was significantly upregulated in tumor tissue and plasma of HCC patients, and its expression level was highly correlated with gender and microvascular invasion. Functionally, miR-92b could promote cell proliferation and metastasis of HCC in vitro and in vivo. Mechanistic investigations suggested that Smad7, which exhibited an inverse relationship with miR-92b expression in HCC, was a direct target of miR-92b and could reverse its effects on HCC tumorigenesis. Furthermore, long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) and miR-92b could directly interact with and repress each other, and XIST could inhibit HCC cell proliferation and metastasis by targeting miR-92b. Taken together, our study not only revealed for the first time the importance of XIST/miR-92b/Smad7 signaling axis in HCC progression but also suggested the potential value of miR-92b as a biomarker in the clinical diagnosis and treatment of HCC.
A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Authors: Wang Z , Liu D , Varin A , Nicolas V , Courilleau D , Mateo P , Caubere C , Rouet P , Gomez A-M , Vandecasteele G , Fischmeister R , Brenner C 
Published: April 2016

AbstractAlthough cardiac cytosolic cyclic 3′,5′-adenosine monophosphate (cAMP) regulates multiple processes, such as beating, contractility, metabolism and apoptosis, little is known yet on the role of this second messenger within cardiac mitochondria. Using cellular and subcellular approaches, we demonstrate here the local expression of several actors of cAMP signaling within cardiac mitochondria, namely a truncated form of soluble AC (sACt) and the exchange protein directly activated by cAMP 1 (Epac1), and show a protective role for sACt against cell death, apoptosis as well as necrosis in primary cardiomyocytes. Upon stimulation with bicarbonate (HCO3−) and Ca2+, sACt produces cAMP, which in turn stimulates oxygen consumption, increases the mitochondrial membrane potential (ΔΨm) and ATP production. cAMP is rate limiting for matrix Ca2+ entry via Epac1 and the mitochondrial calcium uniporter and, as a consequence, prevents mitochondrial permeability transition (MPT). The mitochondrial cAMP effects involve neither protein kinase A, Epac2 nor the mitochondrial Na+/Ca2+ exchanger. In addition, in mitochondria isolated from failing rat hearts, stimulation of the mitochondrial cAMP pathway by HCO3− rescued the sensitization of mitochondria to Ca2+-induced MPT. Thus, our study identifies a link between mitochondrial cAMP, mitochondrial metabolism and cell death in the heart, which is independent of cytosolic cAMP signaling. Our results might have implications for therapeutic prevention of cell death in cardiac pathologies.
Cell cycle and apoptosis regulation by NFAT transcription factors: new roles for an old player
Authors: Mognol G P , Carneiro F R G , Robbs B K , Faget D V , Viola J P B 
Published: April 2016

AbstractThe NFAT (nuclear factor of activated T cells) family of transcription factors consists of four Ca2+-regulated members (NFAT1–NFAT4), which were first described in T lymphocytes. In addition to their well-documented role in T lymphocytes, where they control gene expression during cell activation and differentiation, NFAT proteins are also expressed in a wide range of cells and tissue types and regulate genes involved in cell cycle, apoptosis, angiogenesis and metastasis. The NFAT proteins share a highly conserved DNA-binding domain (DBD), which allows all NFAT members to bind to the same DNA sequence in enhancers or promoter regions. The same DNA-binding specificity suggests redundant roles for the NFAT proteins, which is true during the regulation of some genes such asIL-2andp21. However, it has become increasingly clear that different NFAT proteins and even isoforms can have unique functions. In this review, we address the possible reasons for these distinct roles, particularly regarding N- and C-terminal transactivation regions (TADs) and the partner proteins that interact with these TADs. We also discuss the genes regulated by NFAT during cell cycle regulation and apoptosis and the role of NFAT during tumorigenesis.
Untangling the brain’s neuroinflammatory and neurodegenerative transcriptional responses
Authors: Srinivasan Karpagam , Friedman Brad A. , Larson Jessica L. , Lauffer Benjamin E. , Goldstein Leonard D. , Appling Laurie L. , Borneo Jovencio , Poon Chungkee , Ho Terence , Cai Fang , Steiner Pascal , van der Brug Marcel P. , Modrusan Zora , Kaminker Joshua S. , Hansen David V. 
Published: April 2016

AbstractA common approach to understanding neurodegenerative disease is comparing gene expression in diseased versus healthy tissues. We illustrate that expression profiles derived from whole tissue RNA highly reflect the degenerating tissues’ altered cellular composition, not necessarily transcriptional regulation. To accurately understand transcriptional changes that accompany neuropathology, we acutely purify neurons, astrocytes and microglia from single adult mouse brains and analyse their transcriptomes by RNA sequencing. Using peripheral endotoxemia to establish the method, we reveal highly specific transcriptional responses and altered RNA processing in each cell type, with Tnfr1 required for the astrocytic response. Extending the method to an Alzheimer’s disease model, we confirm that transcriptomic changes observed in whole tissue are driven primarily by cell type composition, not transcriptional regulation, and identify hundreds of cell type-specific changes undetected in whole tissue RNA. Applying similar methods to additional models and patient tissues will transform our understanding of aberrant gene expression in neurological disease.
Size-dependent phase transition in methylammonium lead iodide perovskite microplate crystals
Authors: Li Dehui , Wang Gongming , Cheng Hung-Chieh , Chen Chih-Yen , Wu Hao , Liu Yuan , Huang Yu , Duan Xiangfeng 
Published: April 2016

AbstractMethylammonium lead iodide perovskite has attracted considerable recent interest for solution processable solar cells and other optoelectronic applications. The orthorhombic-to-tetragonal phase transition in perovskite can significantly alter its optical, electrical properties and impact the corresponding applications. Here, we report a systematic investigation of the size-dependent orthorhombic-to-tetragonal phase transition using a combined temperature-dependent optical, electrical transport and transmission electron microscopy study. Our studies of individual perovskite microplates with variable thicknesses demonstrate that the phase transition temperature decreases with reducing microplate thickness. The sudden decrease of mobility around phase transition temperature and the presence of hysteresis loops in the temperature-dependent mobility confirm that the orthorhombic-to-tetragonal phase transition is a first-order phase transition. Our findings offer significant fundamental insight on the temperature- and size-dependent structural, optical and charge transport properties of perovskite materials, and can greatly impact future exploration of novel electronic and optoelectronic devices from these materials.
Location-dependent synaptic plasticity rules by dendritic spine cooperativity
Authors: Weber Jens P. , Andrásfalvy Bertalan K. , Polito Marina , Magó Ádám , Ujfalussy Balázs B. , Makara Judit K. 
Published: April 2016

AbstractNonlinear interactions between coactive synapses enable neurons to discriminate between spatiotemporal patterns of inputs. Using patterned postsynaptic stimulation by two-photon glutamate uncaging, here we investigate the sensitivity of synaptic Ca2+ signalling and long-term plasticity in individual spines to coincident activity of nearby synapses. We find a proximodistally increasing gradient of nonlinear NMDA receptor (NMDAR)-mediated amplification of spine Ca2+ signals by a few neighbouring coactive synapses along individual perisomatic dendrites. This synaptic cooperativity does not require dendritic spikes, but is correlated with dendritic Na+ spike propagation strength. Furthermore, we show that repetitive synchronous subthreshold activation of small spine clusters produces input specific, NMDAR-dependent cooperative long-term potentiation at distal but not proximal dendritic locations. The sensitive synaptic cooperativity at distal dendritic compartments shown here may promote the formation of functional synaptic clusters, which in turn can facilitate active dendritic processing and storage of information encoded in spatiotemporal synaptic activity patterns.
Nanoscale electron transport at the surface of a topological insulator
Authors: Bauer Sebastian , Bobisch Christian A. 
Published: April 2016

AbstractThe use of three-dimensional topological insulators for disruptive technologies critically depends on the dissipationless transport of electrons at the surface, because of the suppression of backscattering at defects. However, in real devices, defects are unavoidable and scattering at angles other than 180° is allowed for such materials. Until now, this has been studied indirectly by bulk measurements and by the analysis of the local density of states in close vicinity to defect sites. Here, we directly measure the nanoscale voltage drop caused by the scattering at step edges, which occurs if a lateral current flows along a three-dimensional topological insulator. The experiments were performed using scanning tunnelling potentiometry for thin Bi2Se3 films. So far, the observed voltage drops are small because of large contributions of the bulk to the electronic transport. However, for the use of ideal topological insulating thin films in devices, these contributions would play a significant role.
In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins
Authors: Shu Changlong , Zhou Jianqiao , Crickmore Neil , Li Xianchun , Song Fuping , Liang Gemei , He Kanglai , Huang Dafang , Zhang Jie 
Published: April 2016

AbstractDuring evolution the creation of single crossover chimeras between duplicated paralogous genes is a known process for increasing diversity. Comparing the properties of homologously recombined chimeras with one or two crossovers is also an efficient strategy for analyzing relationships between sequence variation and function. However, no well-developed in vitro method has been established to create single-crossover libraries. Here we present an in vitro template-change polymerase change reaction that has been developed to enable the production of such libraries. We applied the method to two closely related toxin genes from B. thuringiensis and created chimeras with differing properties that can help us understand how these toxins are able to differentiate between insect species.
Diffraction-free beams in fractional Schrödinger equation
Authors: Zhang Yiqi , Zhong Hua , Belić Milivoj R. , Ahmed Noor , Zhang Yanpeng , Xiao Min 
Published: April 2016

AbstractWe investigate the propagation of one-dimensional and two-dimensional (1D, 2D) Gaussian beams in the fractional Schrödinger equation (FSE) without a potential, analytically and numerically. Without chirp, a 1D Gaussian beam splits into two nondiffracting Gaussian beams during propagation, while a 2D Gaussian beam undergoes conical diffraction. When a Gaussian beam carries linear chirp, the 1D beam deflects along the trajectories z = ±2(x − x0), which are independent of the chirp. In the case of 2D Gaussian beam, the propagation is also deflected, but the trajectories align along the diffraction cone "Equation missing" and the direction is determined by the chirp. Both 1D and 2D Gaussian beams are diffractionless and display uniform propagation. The nondiffracting property discovered in this model applies to other beams as well. Based on the nondiffracting and splitting properties, we introduce the Talbot effect of diffractionless beams in FSE.
Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet and their expression in rice
Authors: Muthamilarasan Mehanathan , Mangu Venkata R. , Zandkarimi Hana , Prasad Manoj , Baisakh Niranjan 
Published: April 2016

AbstractADP-ribosylation factors (ARFs) have been reported to function in diverse physiological and molecular activities. Recent evidences also demonstrate the involvement of ARFs in conferring tolerance to biotic and abiotic stresses in plant species. In the present study, 23 and 25 ARF proteins were identified in C3model- rice and C4model- foxtail millet, respectively. These proteins are classified into four classes (I–IV) based on phylogenetic analysis, with ARFs in classes I–III and ARF-like proteins (ARLs) in class IV. Sequence alignment and domain analysis revealed the presence of conserved and additional motifs, which may contribute to neo- and sub-functionalization of these proteins. Promoter analysis showed the presence of severalcis-regulatory elements related to stress and hormone response, indicating their role in stress regulatory network. Expression analysis of riceARFsandARLsin different tissues, stresses and abscisic acid treatment highlighted temporal and spatial diversification of gene expression. Five rice cultivars screened for allelic variations inOsARFgenes showed the presence of allelic polymorphisms in few gene loci. Altogether, the study provides insights on characteristics ofARF/ARLgenes in rice and foxtail millet, which could be deployed for further functional analysis to extrapolate their precise roles in abiotic stress responses.
HSP27 and 70 expression in thymic epithelial tumors and benign thymic alterations: diagnostic, prognostic and physiologic implications
Authors: Janik S. , Schiefer A. I. , Bekos C. , Hacker P. , Haider T. , Moser J. , Klepetko W. , Müllauer L. , Ankersmit H. J. , Moser B. 
Published: April 2016

AbstractThymic Epithelial Tumors (TETs), the most common tumors in the anterior mediastinum in adults, show a unique association with autoimmune Myasthenia Gravis (MG) and represent a multidisciplinary diagnostic and therapeutic challenge. Neither risk factors nor established biomarkers for TETs exist. Predictive and diagnostic markers are urgently needed. Heat shock proteins (HSPs) are upregulated in several malignancies promoting tumor cell survival and metastases. We performed immunohistochemical staining of HSP27 and 70 in patients with TETs (n = 101) and patients with benign thymic alterations (n = 24). Further, serum HSP27 and 70 concentrations were determined in patients with TETs (n = 46), patients with benign thymic alterations (n = 33) and volunteers (n = 49) by using ELISA. HSPs were differentially expressed in histologic types and pathological tumor stages of TETs. Weak HSP tumor expression correlated with worse freedom from recurrence. Serum HSP concentrations were elevated in TETs and MG, correlated with clinical tumor stage and histologic subtype and decreased significantly after complete tumor resection. To conclude, we found HSP expression in the vast majority of TETs, in physiologic thymus and staining intensities in patients with TETs have been associated with prognosis. However, although interesting and promising the role of HSPs in TETs as diagnostic and prognostic or even therapeutic markers need to be further evaluated.
From molecular interaction to acute promyelocytic leukemia: Calculating leukemogenesis and remission from endogenous molecular-cellular network
Authors: Yuan Ruoshi , Zhu Xiaomei , Radich Jerald P. , Ao Ping 
Published: April 2016

AbstractAcute promyelocytic leukemia (APL) remains the best example of a malignancy that can be cured clinically by differentiation therapy. We demonstrate that APL may emerge from a dynamical endogenous molecular-cellular network obtained from normal, non-cancerous molecular interactions such as signal transduction and translational regulation under physiological conditions. This unifying framework, which reproduces APL, normal progenitor, and differentiated granulocytic phenotypes as different robust states from the network dynamics, has the advantage to study transition between these states, i.e. critical drivers for leukemogenesis and targets for differentiation. The simulation results quantitatively reproduce microarray profiles of NB4 and HL60 cell lines in response to treatment and normal neutrophil differentiation, and lead to new findings such as biomarkers for APL and additional molecular targets for arsenic trioxide therapy. The modeling shows APL and normal states mutually suppress each other, both in “wiring” and in dynamical cooperation. Leukemogenesis and recovery under treatment may be a consequence of spontaneous or induced transitions between robust states, through “passes” or “dragging” by drug effects. Our approach rationalizes leukemic complexity and constructs a platform towards extending differentiation therapy by performing “dry” molecular biology experiments.
Abrupt current switching in graphene bilayer tunnel transistors enabled by van Hove singularities
Authors: Alymov Georgy , Vyurkov Vladimir , Ryzhii Victor , Svintsov Dmitry 
Published: April 2016

AbstractIn a continuous search for the energy-efficient electronic switches, a great attention is focused on tunnel field-effect transistors (TFETs) demonstrating an abrupt dependence of the source-drain current on the gate voltage. Among all TFETs, those based on one-dimensional (1D) semiconductors exhibit the steepest current switching due to the singular density of states near the band edges, though the current in 1D structures is pretty low. In this paper, we propose a TFET based on 2D graphene bilayer which demonstrates a record steep subthreshold slope enabled by van Hove singularities in the density of states near the edges of conduction and valence bands. Our simulations show the accessibility of 3.5 × 104 ON/OFF current ratio with 150 mV gate voltage swing and a maximum subthreshold slope of (20 μV/dec)−1 just above the threshold. The high ON-state current of 0.8 mA/μm is enabled by a narrow (~0.3 eV) extrinsic band gap, while the smallness of the leakage current is due to an all-electrical doping of the source and drain contacts which suppresses the band tailing and trap-assisted tunneling.
Liquid–liquid phase separation of N-isopropylpropionamide aqueous solutions above the lower critical solution temperature
Authors: Mochizuki Kenji , Sumi Tomonari , Koga Kenichiro 
Published: April 2016

AbstractWe investigate driving forces of the liquid–liquid phase separation of N-isopropylpropionamide (NiPPA) aqueous solutions above the lower critical solution temperature using molecular dynamics simulations. Spontaneous phase separations of the model aqueous solution with a modified OPLS-AA force field are observed above the experimentally determined cloud point. The destabilization toward the phase separation is confirmed by temperature dependence of the long-wavelength limit of the concentration-concentration structure factor, the dominant component of which is found to be an increasing effective attraction between NiPPA molecules. At varying temperatures, the potentials of mean force (PMFs) between a pair of NiPPA molecules at infinite dilution are obtained and decomposed into the nonpolar and Coulombic contributions. The nonpolar contribution, arising essentially from molecular volume, promotes association of NiPPA molecules with increasing temperature while the Coulombic one antagonizes the association. Thus, our analysis leads to a conclusion that the driving force of thermally induced aggregation of NiPPA molecules is the temperature dependence of the nonpolar contribution in PMF between NiPPA molecules, not the temperature dependence of the number or strength of hydrogen bonds between NiPPA and water molecules.