bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Exposure to Tris(1,3-dichloro-2-propyl) Phosphate (TDCIPP) Causes Hepatic Inflammation and Leads to Hepatotoxicity in Zebrafish
Authors: Liu Chunsheng , Su Guanyong , Giesy John P. , Letcher Robert J. , Li Guangyu , Agrawal Ira , Li Jing , Yu Liqin , Wang Jianghua , Gong Zhiyuan 
Published: January 2016

AbstractTris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been frequently detected in environmental media and has adverse health effect on wildlife and humans. It has been implicated to have hepatotoxicity, but its molecular mechanisms remain unclear. In the present study, adult male zebrafish were exposed to TDCIPP and global hepatic gene expression was examined by RNA-Seq and RT-qPCR in order to understand the molecular mechanisms of TDCIPP-induced hepatotoxicity. Our results indicated that TDCIPP exposure significantly up-regulated the expression of genes involved in endoplasmic reticulum stress and Toll-like receptor (TLR) pathway, implying an inflammatory response, which was supported by up-regulation of inflammation-related biomaker genes. Hepatic inflammation was further confirmed by histological observation of increase of infiltrated neutrophils and direct observation of liver recruitment of neutrophils labeled with Ds-Red fluorescent protein of Tg(lysC:DsRed) zebrafish upon TDCIPP exposure. To further characterize the hepatotoxicity of TDCIPP, the expression of hepatotoxicity biomarker genes, liver histopathology and morphology were examined. The exposure to TDCIPP significantly up-regulated the expression of several biomarker genes for hepatotoxicity (gck, gsr and nqo1) and caused hepatic vacuolization and apoptosis as well as increase of the liver size. Collectively, our results suggest that exposure to TDCIPP induces hepatic inflammation and leads to hepatotoxicity in zebrafish.
Dihydroartemisinin prevents breast cancer-induced osteolysis via inhibiting both breast caner cells and osteoclasts
Authors: Feng Ming-Xuan , Hong Jian-Xin , Wang Qiang , Fan Yong-Yong , Yuan Chi-Ting , Lei Xin-Huan , Zhu Min , Qin An , Chen Hai-Xiao , Hong Dun 
Published: January 2016

AbstractBone is the most common site of distant relapse in breast cancer, leading to severe complications which dramatically affect the patients’ quality of life. It is believed that the crosstalk between metastatic breast cancer cells and osteoclasts is critical for breast cancer-induced osteolysis. In this study, the effects of dihydroartemisinin (DHA) on osteoclast formation, bone resorption, osteoblast differentiation and mineralization were initially assessed in vitro, followed by further investigation in a titanium-particle-induced osteolysis model in vivo. Based on the proved inhibitory effect of DHA on osteolysis, DHA was further applied to MDA-MB-231 breast cancer-induced mouse osteolysis model, with the underlying molecular mechanisms further investigated. Here, we verified for the first time that DHA suppressed osteoclast differentiation, F-actin ring formation and bone resorption through suppressing AKT/SRC pathways, leading to the preventive effect of DHA on titanium-particle-induced osteolysis without affecting osteoblast function. More importantly, we demonstrated that DHA inhibited breast tumor-induced osteolysis through inhibiting the proliferation, migration and invasion of MDA-MB-231 cells via modulating AKT signaling pathway. In conclusion, DHA effectively inhibited osteoclastogenesis and prevented breast cancer-induced osteolysis.
Biochemical Characterization of Nonamer Binding Domain of RAG1 Reveals its Thymine Preference with Respect to Length and Position
Authors: Raveendran Deepthi , Raghavan Sathees C. 
Published: January 2016

AbstractRAG complex consisting of RAG1 and RAG2 is a site-specific endonuclease responsible for the generation of antigen receptor diversity. It cleaves recombination signal sequence (RSS), comprising of conserved heptamer and nonamer. Nonamer binding domain (NBD) of RAG1 plays a central role in the recognition of RSS. To investigate the DNA binding properties of the domain, NBD of murine RAG1 was cloned, expressed and purified. Electrophoretic mobility shift assays showed that NBD binds with high affinity to nonamer in the context of 12/23 RSS or heteroduplex DNA. NBD binding was specific to thymines when single stranded DNA containing poly A, C, G or T were used. Biolayer interferometry studies showed that poly T binding to NBD was robust and comparable to that of 12RSS. More than 23 nt was essential for NBD binding at homothymidine stretches. On a double-stranded DNA, NBD could bind to A:T stretches, but not G:C or random sequences. Although NBD is indispensable for sequence specific activity of RAGs, external supplementation of purified nonamer binding domain to NBD deleted cRAG1/cRAG2 did not restore its activity, suggesting that the overall domain architecture of RAG1 is important. Therefore, we define the sequence requirements of NBD binding to DNA.
Precise Analysis of Polymer Rotational Dynamics
Authors: Kim Jun Mo , Baig Chunggi 
Published: January 2016

AbstractThrough the analysis of individual chain dynamics alongside the corresponding molecular structures under shear via nonequilibrium molecular dynamics simulations of C178H358 linear and short-chain branched polyethylene melts under shear flow, we observed that the conventional method based on the chain end-to-end vector (and/or the gyration tensor of chain) is susceptible to quantitatively inaccurate measurements and often misleading information in describing the rotational dynamics of polymers. Identifying the flaw as attributed to strong irregular Brownian fluctuations inherent to the chain ends associated with their large free volume and strong molecular collisions, we propose a simple, robust way based on the chain center-to-center vector connecting the two centers of mass of the bisected chain, which is shown to adequately describe polymer rotational dynamics without such shortcomings. We present further consideration that the proposed method can be useful in accurately measuring the overall chain structure and dynamics of polymeric materials with various molecular architectures, including branched and ring polymers.
Altered functional connectivity in persistent developmental stuttering
Authors: Yang Yang , Jia Fanlu , Siok Wai Ting , Tan Li Hai 
Published: January 2016

AbstractPersistent developmental stuttering (PDS) is a speech disorder that impairs communication skills. Despite extensive research, the core causes of PDS are elusive. Converging evidence from task-induced neuroimaging methods has demonstrated the contributions of the basal ganglia and the cerebellum to PDS, but such task-state neuroimaging findings are often confounded by behavioral performance differences between subjects who stutter and normal controls. Here, using resting-state functional magnetic resonance imaging, we investigated functional connectivity within cerebellar-cortical and basal ganglia-thalamocortical networks in 16 adults who stutter and 18 age-matched fluent speakers. Seed-to-voxel analysis demonstrated that, compared to controls, adults who stutter showed alternations in functional connectivity of cerebellum to motor cortex as well as connectivity among different locals within cerebellum. Additionally, we found that functional connectivity within cerebellar circuits was significantly correlated with severity of stuttering. The alternations of functional connectivity within basal ganglia-thalamocortical networks were identified as the reduced connectivity of the putamen to the superior temporal gyrus and inferior parietal lobules in adults who stutter. The abnormalities of resting state functional connectivity are assumed to affect language planning and motor execution critical for speaking fluently. Our findings may yield neurobiological cues to the biomarkers of PDS.
Selenium Supplementation Affects Insulin Resistance and Serum hs-CRP in Patients with Type 2 Diabetes and Coronary Heart Disease
Authors: Farrokhian A. , Bahmani F. , Taghizadeh M. , Mirhashemi S. M. , Aarabi MH , Raygan F. , Aghadavod E. , Asemi Z. 
Published: January 2016

AbstractTo our knowledge, this study is the first indicating the effects of selenium supplementation on metabolic status of patients with type 2 diabetes mellitus (T2DM) and coronary heart disease (CHD). This study was conducted to evaluate the effects of selenium supplementation on metabolic profiles, biomarkers of inflammation, and oxidative stress of patients with T2DM and CHD. This randomized, double-blind, placebo-controlled trial was performed among 60 patients with T2DM and CHD aged 40–85 years. Participants were randomly divided into 2 groups. Group A received 200 μg selenium supplements (n=30) and group B received placebo per day (n=30) for 8 weeks. Fasting blood samples were taken at the beginning of the study and after 8-week intervention to quantify metabolic profiles. After 8 weeks, compared with the placebo, selenium supplementation resulted in a significant decrease in serum insulin levels (− 2.2±4.6 vs. + 3.6±8.4 μIU/ml, p=0.001), homeostasis model of assessment-insulin resistance (HOMA-IR) (− 0.7±1.3 vs. + 0.9±2.4, p=0.004), homeostatic model assessment-beta cell function (HOMA-B) (− 7.5±17.2 vs. + 15.1±34.5, p=0.002) and a significant increase in quantitative insulin sensitivity check index (QUICKI) (+0.01±0.03 vs. − 0.01±0.03, p=0.02). In addition, patients who received selenium supplements had a significant reduction in serum high-sensitivity C-reactive protein (hs-CRP) (− 1 372.3±2 318.8 vs. − 99.8±1 453.6 ng/ml, p=0.01) and a significant rise in plasma total antioxidant capacity (TAC) concentrations (+ 301.3±400.6 vs. − 127.2±428.0 mmol/l, p<0.001) compared with the placebo. A 200 μg/day selenium supplementation among patients with T2DM and CHD resulted in a significant decrease in insulin, HOMA-IR, HOMA-B, serum hs-CRP, and a significant increase in QUICKI score and TAC concentrations.
Winter Diets of Brown Trout Populations in Southeastern Minnesota and the Significance of Winter‐Emerging Invertebrates
Authors: Anderson A. M. , Mittag E. , Middleton B. , Vondracek B. , Ferrington L. C. 
Published: January 2016

AbstractWinter is a critical period for stream‐dwelling trout, and the need to extend investigations into this period has long been recognized. The intent of this research was to investigate the winter diet of Brown TroutSalmo trutta, as only a limited number of studies have focused on winter dynamics and how the winter macroinvertebrate community affects trout during winter. Our specific objectives were to assess variability in winter diet of different size‐classes of trout, determine whether fish exhibit size‐selective predation, quantify the extent that trout differentially exhibit drift or benthic feeding, and assess the importance of winter‐emerging insects in the winter diet. Stomach contents from 30 Brown Trout were collected from three streams on six occasions during the winter of 2010 and compared with macroinvertebrates collected in the drift and from the benthos. Trout in each stream exhibited distinct diets, and diets of larger fish differed from those of smaller fish; larger trout consumed greater amounts of Trichoptera andPhysellaand smaller fish ingested moreGammarusand Chironomidae larvae. Stomach contents of both sizes of fish were more similar to the benthos than drift, indicating fish had a greater reliance on benthic feeding in winter. When considered across all streams, trout preferentially selected Trichoptera and Chironomidae and maintained size‐selective predation throughout the winter, selecting largerGammarusand Chironomidae larvae than typically found in the environment. However, winter‐emerging Chironomidae appeared to enhance diets in all three streams, and chironomid larvae were particularly dominant in the diet of one trout population. Variations in abundance of winter‐emerging insects between streams may impact the winter condition of Brown Trout, particularly in smaller fish, which were found to feed more heavily on these organisms. These findings may have important implications for fisheries management.
Trunk Muscle Training Augmented With Neuromuscular Electrical Stimulation Appears to Improve Function in Older Adults With Chronic Low Back Pain
Authors: Hicks Gregory E. , Sions Jaclyn M. , Velasco Teonette O. , Manal Tara J. 
Published: January 2016

Objectives:
To assess the feasibility of a trial to evaluate a trunk muscle training program augmented with neuromuscular electrical stimulation (TMT+NMES) for the rehabilitation of older adults with chronic low back pain (LBP) and to preliminarily investigate whether TMT+NMES could improve physical function and pain compared with a passive control intervention.

Materials and Methods:
We conducted a single-blind, randomized feasibility trial. Patients aged 60 to 85 years were allocated to TMT+NMES (n=31) or a passive control intervention (n=33), consisting of passive treatments, that is, heat, ultrasound, and massage. Outcomes assessed 3- and 6-month postrandomization included Timed Up and Go Test, gait speed, pain, and LBP-related functional limitation.

Results:
Feasibility was established by acceptable adherence (≥80%) and attrition (<20%) rates for both interventions. Both groups had similar, clinically important reductions in pain of >2 points on a numeric pain rating scale during the course of the trial. But, only the TMT+NMES group had clinically important improvements in both performance-based and self-reported measures of function. In terms of the participants’ global rating of functional improvement at 6 months, the TMT+NMES group improved by 73.9% and the passive control group improved by 56.7% compared with baseline. The between-group difference was 17.2% (95% confidence interval, 5.87-28.60) in favor of TMT+NMES.

Discussion:
It seems that a larger randomized trial investigating the efficacy of TMT+NMES for the purpose of improving physical function in older adults with chronic LBP is warranted.
Monothermal Caloric Screening to Improve Healthcare Value
Authors: Thatcher Aaron L. , Beckerman Margot L. , Telian Steven A. , King William Michael 
Published: January 2016

Objectives:To evaluate whether monothermal caloric screening can reduce the number of caloric irrigations required in the vestibular testing battery while maintaining diagnostic accuracy.Design:Prospective controlled cohort study. Three hundred and ninety patients referred for vestibular testing at this tertiary referral health system over a 1-year period were evaluated; 24 patients met exclusion or failure criteria and 366 patients were included in the study. Population was 35.6% male; average age was 50.4 years old. Each patient underwent caloric testing using either warm or cool water irrigation initially and this data was used for monothermal screening data. All patients then completed bithermal binaural caloric testing to obtain the “gold standard” bithermal data for comparison. The sensitivity and specificity of monothermal cool or monothermal warm caloric tests were calculated using a receiver operating characteristic curve analysis.Results:Using a monothermal interear difference threshold of 25%, warm monothermal screening had sensitivity of 98.0%, specificity of 91.3%, false negative rate of 2%, and false positive rate of 8.7%. Cool monothermal screening also had excellent sensitivity (92.3%) and specificity (95.3)%, with a false negative rate of 7.7%, and a false positive rate of 4.7%. The diagnosis associated with the single false negative warm monothermal caloric test was compensated vestibular paresis. In the study population, 71.9% had a negative monothermal screen; if the monothermal data were accepted, 2 fewer irrigations would have been performed resulting in an average saving of $264 (typical Medicare reimbursement for 2 irrigations) billed per patient screened as well as shortening the average testing battery by about 15 min.Conclusions:Warm monothermal caloric screening can reduce time and cost of vestibular testing while nearly matching the diagnostic accuracy of bithermal testing.
A study on collection development and its organizational pattern of university libraries in Uttar Pradesh (India)
Authors: Khan Abdul Mannan 
Published: January 2016

Purpose
– The purpose of the study is to know the collection development and its organizational pattern of university libraries in India.

Design/methodology/approach
– For the purpose of the study, three sets of questionnaire were prepared by the researcher and furnished to librarians and acquisition librarians (collection development in charge) of these referred libraries.

Findings
– The survey reveals that the old centralized universities are better placed in terms of all the parameters of collection development. It is also being revealed that there is a difference between the two old centralized and newly centralized universities especially in Babasaheb Bhimrao Ambedkar University, the de-novo centralized university. It has to do a lot in the sphere of information technology (IT) application and IT-enabled services to the users. Furthermore, there is a conspicuous difference between the two categories that the newly centralized universities are highly lagging behind the old centralized universities in organizational patter of collection development.

Originality/value
– There are a number of studies on collection development in the world, but this is the first of its kind within Indian Universities.
Citation analysis for core journals in educational leadership
Authors: Griffin Karin L 
Published: January 2016

Purpose– The purpose of this paper is to establish a core serials list for “Single doctoral”, Carnegie classification universities offering Doctor of Education (EdD) programs in educational leadership based on a sample of six institutions over a six-year period.Design/methodology/approach– A citation analysis was used using a descriptive research design. The population was all dissertations accepted for the award of the EdD degree in Educational Leadership from 2005 to 2010 at six peer institutions. From this population, 20 dissertations from each institution were randomly selected during the period under study. Data were extracted from the title pages and reference lists. Extracted data were analyzed using descriptive statistics. Serial titles were ranked according to a frequency-weighted index formula, which recorded the serial titles cited by the greatest percentage of students.Findings– It was found that the serials with the highest frequency weight depended, in part, on the period studied. The findings also showed that 84.2 per cent of the serial citations were represented by four Library of Congress classification areas: education (L); social sciences (H); medicine (R) and philosophy, psychology and religion (B). In addition, it was found that EdD students cited serials aged 10 years or less more than other sources of information materials.Originality/value– While other citation analyses of dissertations have been done, including some that compare dissertations at more than one institution, the author could find no other study of EdD dissertations, specifically, across a nationwide group of peer institutions. Further, the author could find no other citation analysis covering as many as six continuous years, which made a difference in the most frequently cited serial titles.
E-resources: use and search strategies adopted by users of Dr Y.S. Parmar University of Horticulture and Forestry
Authors: Bhat Nazir Ahmad , Ganaie Shabir  A 
Published: January 2016

Purpose
– The purpose of this paper is to identify the most popular places, gadgets, searching tools and techniques adopted by users of Dr Y.S. Parmar University of Horticulture and Forestry (DYSPUH
&
F), Nauni, Solan, Himachal Pradesh, while searching electronic information resources (EIRs).

Design/methodology/approach
– A survey was conducted with the help of a structured questionnaire used as a data collection tool.

Findings
– It is found that department/office chamber (42 per cent), hostel (29 per cent) and home (26 per cent) prove to be the most popular places of access. Majority of users use laptops to access and read EIRs. Among all popular platforms, users of DYSPUH
&
F library prefer to use “search engines”, and “Google” proves to be the number one search engine. Majority of users search the information through “title” followed by “keywords/subject terms”. The users are not yet well-versed with most of the advanced search techniques, as less than half of them are able to use only Boolean operators, and less than 10 per cent of them claim to know other search techniques. Majority of users have learnt to use information search and retrieval skills through self-study.

Research limitations/implications
– Only one agricultural university of northern India was surveyed; hence, the results cannot be generalised for the entire northern region of the country.

Practical implications
– The findings will help the library authorities to see what are the prime areas of concern so far as the search discourse and accessibility of e-resources is concerned.

Originality/value
– No such survey has been conducted in the University till date.
Reciprocal benefits, legacy and risk
Authors: Campbell Paul , Evans Peter 
Published: January 2016

Purpose– The purpose of this paper is to explore the beliefs held by managers about their roles as facilitators of learning with their employees in a public utilities organisation.Design/methodology/approach– The research was based on Ellinger and Bostrom’s (2002) study on managers’ beliefs on their role as facilitators of learning in learning-orientated firms. Abductive research logic was used in a small sample in depth qualitative study using critical incident interviews.Findings– Managers in the study conveyed strong self-efficacy and outcome beliefs confirming the central role in workplace learning of line managers who adopt a coaching approach. Key new insights were also found in managers’ beliefs on acting as role models within the organisation and their beliefs on the need to manage skills-related organisational risk.Research limitations/implications– A key limitation of the research is inherent in the use of critical incident technique, as it provides information on the nature of “atypical events” as opposed to more gradual, tacit and typically ongoing learning at work.Practical implications– The managers’ belief map derived from the data provides a context-specific “target of change” with which to challenge the wider organisation regarding learning facilitation. The research also shows how industry-specific contexts may provide specific pathways for developing managers in their role as facilitators of learning.Originality/value– The value of the research is twofold: first, providing further validation of the findings from Ellinger and Bostrom’s (2002) research on managers’ beliefs on the effective facilitation of workplace learning; second, additional insights on managerial beliefs regarding role modelling and succession planning are identified, and the implications for management development are discussed.