bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bridging healthcare gaps: a scoping review on the role of artificial intelligence, deep learning, and large language models in alleviating problems in medical deserts
Authors: Strika Zdeslav , Petkovic Karlo , Likic Robert , Batenburg Ronald 
Published: September 2024

Abstract
“Medical deserts” are areas with low healthcare service levels, challenging the access, quality, and sustainability of care. This qualitative narrative review examines how artificial intelligence (AI), particularly large language models (LLMs), can address these challenges by integrating with e-Health and the Internet of Medical Things to enhance services in under-resourced areas. It explores AI-driven telehealth platforms that overcome language and cultural barriers, increasing accessibility. The utility of LLMs in providing diagnostic assistance where specialist deficits exist is highlighted, demonstrating AI’s role in supplementing medical expertise and improving outcomes. Additionally, the development of AI chatbots offers preliminary medical advice, serving as initial contact points in remote areas. The review also discusses AI’s role in enhancing medical education and training, supporting the professional development of healthcare workers in these regions. It assesses AI’s strategic use in data analysis for effective resource allocation, identifying healthcare provision gaps. AI, especially LLMs, is seen as a promising solution for bridging healthcare gaps in “medical deserts,” improving service accessibility, quality, and distribution. However, continued research and development are essential to fully realize AI’s potential in addressing the challenges of medical deserts.
Vitamin D receptor alleviates lipid peroxidation in diabetic nephropathy by regulating ACLY/Nrf2/Keap1 pathway
Authors: Zhou Yueyi , Liao Qin , Li Dan , Chen Li , Zhang Hao , Yi Bin 
Published: September 2024

AbstractThe membrane lipid damage caused by reactive oxygen species(ROS) and various peroxides, namely lipid peroxidation, plays an important role in the progression of diabetic nephropathy (DN).We previously reported that vitamin D receptor(VDR) plays an active role in DN mice by modulating autophagy disorders. However, it is unclear whether the ATP‐citrate lyase (ACLY)/NF‐E2‐related factor‐2 (Nrf2)/Kelch‐like ECH‐associated protein 1 (Keap1) pathway is associated with the reduction of lipid peroxidation by VDR in the DN model. We found that in the DN mouse model, VDR knockout significantly aggravated mitochondrial morphological damage caused by DN, increased the expression of ACLY, promoted the accumulation of ROS, lipid peroxidation products Malondialdehyde(MDA) and 4‐hydroxy‐2‐nonenal (4‐HNE),consumed the Nrf2/Keap1 system, thus increasing lipid peroxidation. However, the overexpression of VDR and intervention with the VDR agonist paricalcitol (Pari) can reduce the above damage. On the other hand, cellular experiments have shown that Pari can significantly reduce the elevated expression of ACLY and ROS induced by advanced glycation end products (AGE). However, ACLY overexpression partially eliminated the positive effects of the VDR agonist. Next, we verified the transcriptional regulation of ACLY by VDR through chromatin immunoprecipitation (ChIP)‐qPCR and dual luciferase experiments. Moreover, in AGE models, knockdown of ACLY decreased lipid peroxidation and ROS production, while intervention with Nrf2 inhibitor ML385 partially weakened the protective effect of ACLY downregulation. In summary, VDR negatively regulates the expression of ACLY through transcription, thereby affecting the state of Nrf2/Keap1 system and regulating lipid peroxidation, thereby inhibiting kidney injury induced by DN.
Effect of an Enteral Formula Enriched With ω-3 Fatty Acids, Carnitine, and Vitamin D on Body Weight, Heart Weight, and Blood Biochemical Parameters in a Dahl Rat Heart Failure Model
Authors: Ryuno Yoshikazu , Kobayashi Jun-ichi , Fujimoto Yudai , Dotare Taishi , Matsue Yuya , Iwanami Yoshihito 
Published: September 2024

Abstract:
Malnutrition is known to worsen the prognosis of chronic heart failure (HF). To gain information that may be helpful in establishing appropriate nutritional interventions for chronic HF, this study was performed to investigate the efficacy of nutritional management with 2 enteral formulas, EH, with a standard nutritional composition, and ER, fortified with omega-3 fatty acids, vitamin D, and carnitine. Experiments were performed in a Dahl rat HF model. After being fed a standard rodent feed (MF) containing 8% NaCl (high salt-MF [HS-MF]) from 6 to 11 weeks of age, rats were assigned to freeze-dried EH or ER diets with an NaCl concentration of 8% (HS-ER or HS-EH) until 18 weeks of age. Serum albumin was significantly higher at 14 and 17 weeks of age in rats fed the HS-ER and HS-EH diets compared with those remaining on the HS-MF diet. Body weight was also significantly higher at 14 and 17 weeks of age in animals fed the HS-ER diet, showing that nutritional deterioration was prevented. In addition, heart weight was significantly lower at 18 weeks of age in the HS-ER group than that in the HS-MF group, suggesting that cardiac hypertrophy was prevented. This study demonstrated improved nutritional status in a HF model in Dahl rats presumably owing to differences in nutritional composition in the diets. Future studies are needed to explore optimal nutritional management with enteral formulas in patients with chronic HF.
MACULAR VOLUME SCAN AS A MARKER OF ANGIOGRAPHIC UVEITIC RETINAL VASCULITIS
Authors: Alapati Akhila , Kherani Saleema , Begaj Tedi , Janetos Timothy M. , Zhang Kevin , Koreishi Anjum , Goldstein Debra A. 
Published: September 2024

Purpose:
To determine whether optical coherence tomography thickness values from macular volume scans can be used to detect uveitic retinal vasculitis.

Methods:
Retrospective study of patients with noninfectious retinal vasculitis. Fluorescein angiogram and 61-line optical coherence tomography macular volume scans with an overlying Early Treatment Diabetic Retinopathy study grid were obtained. Correlation between 1 mm and 6 mm Early Treatment Diabetic Retinopathy study ring values and posterior pole vasculitis was analyzed. A linear longitudinal model was used to assess the relationship between global retinal vasculitis scores and optical coherence tomography macular thickness parameters.

Results:
Ninety-nine eyes of 54 patients were included. A total of 460 optical coherence tomography and fluorescein angiogram pairs were analyzed. Central (1 mm) macular thickness was weakly correlated with posterior pole vasculitis (correlation coefficient [rho] = 0.09, P = 0.06). Both the mean 6 mm ring (rho = 0.11, P = 0.018) and the average of the superior and inferior (S/I) 6 mm ring values (rho = 0.13, P = 0.006) were strongly correlated with posterior pole leakage. The longitudinal model showed that S/I 6 mm ring values are associated with vasculitis in the posterior pole, mid, and far periphery (P < 0.0001).

Conclusion:
Optical coherence tomography–based macular volume thickness can detect uveitic retinal vasculitis and aid in monitoring disease activity.
Dual Kv7.2/3-TRPV1 modulators inhibit nociceptor hyperexcitability and alleviate pain without target-related side effects
Authors: Raveh Adi , Pen Yefim , Silberman Alon , Peretz Asher , Attali Bernard , Maile Laura , Davidson Steve , Brown Alan D. , Kennedy Jeffrey D. , Belinson Haim 
Published: September 2024

Abstract
Persistent or chronic pain is the primary reason people seek medical care, yet current therapies are either limited in efficacy or cause intolerable side effects. Diverse mechanisms contribute to the basic phenomena of nociceptor hyperexcitability that initiates and maintains pain. Two prominent players in the modulation of nociceptor hyperexcitability are the transient receptor potential vanilloid type 1 (TRPV1) ligand-gated ion channel and the voltage-gated potassium channel, Kv7.2/3, that reciprocally regulate neuronal excitability. Across many drug development programs targeting either TRPV1 or Kv7.2/3, significant evidence has been accumulated to support these as highly relevant targets; however, side effects that are poorly separated from efficacy have limited the successful clinical translation of numerous Kv7.2/3 and TRPV1 drug development programs. We report here the pharmacological profile of 3 structurally related small molecule analogues that demonstrate a novel mechanism of action (MOA) of dual modulation of Kv7.2/3 and TRPV1. Specifically, these compounds simultaneously activate Kv7.2/3 and enable unexpected specific and potent inhibition of TRPV1. This in vitro potency translated to significant analgesia in vivo in several animal models of acute and chronic pain. Importantly, this specific MOA is not associated with any previously described Kv7.2/3 or TRPV1 class-specific side effects. We suggest that the therapeutic potential of this MOA is derived from the selective and specific targeting of a subpopulation of nociceptors found in rodents and humans. This efficacy and safety profile supports the advancement of dual TRPV1-Kv7.2/3 modulating compounds into preclinical and clinical development for the treatment of chronic pain.
COVID-19 Risk Assessment Among Vulnerable Small Business Owners in El Paso County, Texas
Authors: Conway Sadie H. , Vasquez Denise , Mena Kristina D. , Brown Louis D. , Kwon Soyoung , Rios Janelle 
Published: September 2024

Background
Our objective was to deliver actionable, worksite-specific COVID-19 risk assessments and mitigation strategies tailored to vulnerable workers in one of the highest-risk areas in the US.

Methods
Four trained, bilingual (English/Spanish) community health workers (CHWs) recruited small businesses (ie, ≤20 employees) across various industries and executed novel on-site infectious disease risk assessment surveys of at least one employer and one employee.

Results
Of 102 participating businesses (95% Hispanic-owned), 96% were characterized as “high risk” or “very high risk” for disease transmission. All businesses reported implementing at least one practice to reduce disease transmission; however, almost half of businesses lacked at least 13 of the 17 controls identified to mitigate risk.

Conclusions
Tailored, culturally sensitive outreach led by CHWs identified and educated businesses on critical hazards, and these methods may be transferable to similar communities.
Depiction of Homemade Infant Formula and Formula Hacks on Pinterest
Authors: McAdams Rebecca J. , Roberts Kristin J. , Keim Sarah A. , McKenzie Lara B. 
Published: September 2024

Purpose:
To examine Pinterest pins to discern the content, messaging and claims, and engagement with pins regarding formula hacking and homemade formula.

Background:
The American Academy of Pediatrics and the Centers for Disease Control and Prevention discourage formula hacking (ie, preparing formula not according to directions to extend its use) and making homemade infant formula due to its associated health risks. Yet, caregivers are using social media platforms to find information on these topics.

Methods:
This study conducted a content analysis of Pinterest pins related to formula hacking and homemade infant formula identified from the platform in January 2023 by using 14 search terms.

Results:
A total of 63 pins were included in the sample. The topic of all (100.0%) pins was homemade formula. More than one-half (56.0%) of the 25 pins that provided a recipe included an unsafe ingredient, such as raw milk. The most frequently provided reasons to use homemade infant formula were in an emergency (38.2%) and when breastfeeding was not possible (38.2%). The most common claim was that homemade infant formula was healthy (59.6%). The total number of followers for the 63 pins was 861 636.

Conclusions:
Pinterest is widely used by caregivers to find feeding information for their infants. Credible organizations should utilize Pinterest to disseminate safe infant feeding practices supported by the Centers for Disease Control and Prevention and the American Academy of Pediatrics.

Implications:
The results will help nurses advocate for safe infant feeding and help them guide caregivers away from unsafe formula practices found on social media.
The Effects of Laughter Therapy on Perceived Stress and Quality of Life in Women With Breast Cancer Receiving Chemotherapy
Authors: Karakas Hacer , Zorba Bahceli Pinar , Akyol Murat 
Published: September 2024

Background
Women with breast cancer experience various symptoms secondary to chemotherapy that reduce their quality of life and increase their stress levels.

Objective
To evaluate the effects of laughter therapy on perceived stress levels and quality of life in women with breast cancer receiving chemotherapy using a parallel-group randomized controlled pilot trial.

Methods
Forty-two women were randomly assigned to intervention and control groups. The intervention group received 40 minutes of laughter therapy twice a week for 8 weeks. The control group received routine treatment. The women in both groups completed the Perceived Stress Scale and SF-12 Health Survey at baseline, week 5, and week 9 after randomization.

Results
There was a significant decrease in the mean Perceived Stress Scale scores in the intervention group compared with those in the control group at weeks 5 and 9 compared with baseline, and the difference between groups by measurement week was significant (P < .001). Women in the intervention group had higher Physical Component Summary-12 and Mental Component Summary-12 scores in weeks 5 and 9 compared with those in the control group, and there was a significant difference between groups in terms of change in the Physical Component Summary-12 and Mental Component Summary-12 mean scores over time (P < .001).

Conclusion
Laughter therapy is possible to use as a nursing intervention to reduce the perceived stress levels and increase the quality of life of women receiving chemotherapy for breast cancer.

Implications for Practice
Nurses can apply laughter therapy in the symptom management of women receiving chemotherapy.
Does the palpebral morphology influence the tear meniscus height between Caucasian and Asian eyes?
Authors: Guinel Maëlys , Bitton Etty 
Published: September 2024

SIGNIFICANCE
The tear meniscus height (TMH) along the lid margin is a clinical measure in the evaluation of patients with dry eyes and contact lens wearers. The morphology of the eyelid may differ among ethnic groups, potentially impacting the meniscus height, which can be useful for clinicians to optimize patient care.

PURPOSE
The central lower TMH is used as a clinical measure of tear volume in the assessment of contact lens candidates and patients with dry eyes. Ethnic differences in eyelid morphology may influence the measurement of the TMH. Furthermore, with the advent of larger contact lenses, such as scleral lenses, it would be of clinical value to assess the TMH centrally and peripherally. The purpose of this study was to evaluate and compare the TMH at different positions along the palpebral margin between Caucasian and Asian eyes.

METHODS
This prospective study evaluated the lower TMH in five positions (central, temporal and nasal limbus and temporal and nasal periphery) of the right eye using the Keratograph 5M (Oculus) instrument in Caucasian and Asian participants between 10 am and 12 pm. The TMH at each position was taken three times and averaged and analyzed using a 5 × 2 repeated-measures analysis of variance.

RESULTS
Central TMH did not differ significantly (F = 0.02, p=0.88) in Caucasians (n = 20, aged 24.45 [2.30] years, TMH 0.320 [0.052] mm) and Asians (n = 20, aged 22.25 [3.43] years, TMH 0.325 [0.048] mm). A difference was noted with respect to TMH positions along the lid margin (F = 64.17, p<0.001), independent of ethnicity (F = 2.15, p=0.15). A post hoc analysis revealed a significantly higher TMH temporally when compared with centrally or nasally (p<0.001).

CONCLUSIONS
This study demonstrated the similarity of the central TMH and the differences in the peripheral TMH within Caucasian and Asian eyes. This may be clinically relevant when using the Tear Film & Ocular Surface Society Dry Eye Workshop II diagnostic algorithm for dry eyes and when fitting scleral contact lenses. Future studies need to consider that ethnic differences may exist for certain tests in order to personalize the care and management of each patient.
18F-FDG PET Metabolic Parameters for the Prediction of Histological Response to Induction Chemotherapy in Osteosarcoma and Ewing Sarcoma
Authors: Yadgarov Mikhail , Berikashvili Levan , Rakova Elena , Likar Yury 
Published: September 2024

Purpose
This study aimed to evaluate the ability of 18F-FDG PET/CT metabolic parameters to predict the histological response to neoadjuvant chemotherapy in patients with osteosarcoma and Ewing sarcoma.

Patients and Methods
This systematic review and network meta-analysis adhered to the PRISMA-NMA and Cochrane guidelines. Electronic databases were searched from January 2008 to January 2024; this search was supplemented by snowballing methods. The risk of bias was evaluated with QUADAS-2, and evidence certainty was assessed using the GRADE approach. The prognostic value of 18F-FDG PET/CT parameters, including pretreatment and posttreatment SUVs (SUV1, SUV2 and the SUV2/SUV1 ratio), metabolic tumor volume (MTV1, MTV2, ΔMTV), and total lesion glycolysis (TLG1, TLG2, ΔTLG), was examined.

Results
The meta-analysis of 18 studies (714 patients) identified the ΔTLG, ΔMTV, and SUVratio as superior predictors of histological response. The changes in metabolic activity, as indicated by these parameters, provided a robust indication of treatment effectiveness. Baseline parameters showed limited predictive value compared with posttreatment assessments. The study’s robustness was confirmed through meta-regression, which revealed that the predictive value of the SUV2 and SUVratio was consistent across various cutoff thresholds.

Conclusions

18F-FDG PET/CT metabolic parameters, particularly those measuring changes posttherapy, are effective in predicting the histological response in patients with osteosarcoma and Ewing sarcoma. These findings underscore the potential of 18F-FDG PET/CT in guiding early treatment decisions, thereby enhancing personalized therapeutic approaches.
A shift between r and K strategies in the life cycle of a unicellular microalga
Authors: Liu Lu , Tang Si , Liu Yaqing , Zhang Boya , Zhong Yanlin , Liu Dai , Zhou Jin , Cai Zhonghua 
Published: September 2024

Abstractr (high reproductivity but stress-sensitive offspring) and K (low reproductivity but high stress-resistant offspring) strategies are two classical life history theories. Contrary to the belief that one species only has a single strategy (i.e., either r or K), we present experimental evidence for the coexistence of both strategies in the isogenic microalgaHaematococcus lacustris. Under standard conditions, swimming vegetative cells (SVC) normally grew and divided via binary fission (r strategy) and gradually shifted into non-swimming cysts (NSC) over time. Intriguingly, unlike the prevailing notion that NSC cannot propagate under stress, they were found to reproduce via multiple fission at a barely detectable growth rate, resulting in stress-resistant, non-mobile daughter cells and demonstrating a complete life history (K strategy). Collectively, our findings indicate thatH. lacustrisadopts both r and K strategies throughout its life history. This research enhances the understanding of the adaptation and survival of microbial populations in changing environments.
Tau condensation on DNA and localization on centromeres: A potential link to cell division
Authors: Park Celine , Jung Jaehun , Hong Yuri , Lee-Eom Chaelin , Rah Sang-Hyun , Yang Keunsang , Shin Jaehyeon , Jeong Ayoung , Hong Seokyun , Lee Jong-Bong , Hwang Dong Soo , Shon Min Ju 
Published: September 2024

AbstractTau protein, traditionally recognized for stabilizing microtubules and forming pathological aggregates, has recently been observed to form condensates in various contexts. While its condensation with RNA has been well studied, the interaction between tau and DNA, along with its biological significance, remains less explored. Here, using single-molecule experiments, we found that tau binds stably to naked DNA at nanomolar concentrations, leading to the local co- condensation of tau and DNA. These tau condensates on DNA can also interface with microtubules, leveraging tau’s known role in promoting microtubule growth and organization. The dynamic nature of these condensates facilitates the remodeling of the DNA–microtubule assembly. Interestingly, two phosphomimetic tau mutants, T231D/S235D and S262D, retained their affinity for DNA but differed in their ability to link microtubules to DNA. Furthermore, imaging of HEK-293 and SH-SY5Y cells in early mitosis revealed that tau localizes on centromeres, poised to capture nascent mitotic spindles. Building on these observations, we speculate that tau may play a novel role in mitosis, where tau clusters facilitate the early registration of mitotic spindles to chromosomes before kinetochore-mediated attachment. We also discuss the potential implications of this model in conditions where abnormal cell cycle re-entry and tau activity may disrupt cell division.
A scalable, high-throughput neural development platform identifies shared impact of ASD genes on cell fate and differentiation
Authors: Wang Xuran , Lalli Matthew , Thopte Urvashi , Buxbaum Joseph D. 
Published: September 2024

AbstractBackgroundDeleterious mutations in hundreds of genes confer high risk for neurodevelopmental disorders (NDDs), posing significant challenges for therapeutic development. Identifying convergent pathways shared across NDD genes could reveal high-impact therapeutic targets.MethodsTo identity convergent pathways in NDD genes, we optimized Perturb-seq, a method combining CRISPR perturbation with single-cell RNA sequencing (scRNA-seq), and applied structural topic modeling (STM) to simultaneously assess impact on cell fate and developmental stage. We then studied a subset of autism spectrum disorder (ASD) genes implicated in regulation of gene expression using these improved molecular and analytical approaches.ResultsResults from targeting 60 high-confidence ASD risk genes revealed significant effects on neural development. As expected, ASD risk genes impacted both progenitor fate and/or neuronal differentiation. Using STM, we could identify latent topics jointly capturing cell types, cell fate, and differentiation stages. Repression of ASD risk genes led to changes in topic proportions and effects of four genes (DEAF1,KMT2A,MED13L, andMYT1L)were validated in an independent dataset.ConclusionsOur optimized Perturb-seq method, combined with a novel analytical approach, provides a powerful, cost-effective framework for uncovering convergent mechanisms among genes involved in complex neurodevelopmental processes. Application of these methods advanced understanding of the impact of ASD mutations on multiple dimensions of neural development, and provides a framework for a broader examination of the function of NDD risk genes.