bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of edible film prepared with plasma‐activated water and olive leaf extract (Olea europaea L.) as a potential packaging in cooked meat product
Authors: Bilecen Şen Damla , Güleç Ali 
Published: September 2024

AbstractThis research determined the use of edible film (EF) prepared with olive leaf extract (OLE) and plasma‐activated water (PAW) on the shelf life and quality of cooked meat (M. longissimus thoracis et lumborum) product. The characterization of PAW composition and the antioxidant activity and the total phenolic content (TPC) of OLE were determined. Also, physicochemical and microbiological properties and lipid oxidation of cooked meat product coated with EF stored at +4°C for 14 d were evaluated. Cold plasma treatment increased the nitrite/nitrate concentrations and ORP value and decreased the pH value of distilled water (DW). The amount of TPC, IC50 value, and the antioxidant activity of OLE were 71.52 mg GAE/g, 0.46 mg/mL, and 77.39%, respectively. The combined use of OLE and PAW in EF during storage reduced the pH of cooked meat product. Furthermore, the addition of OLE in EF increased the b* values and reduced the a* values of cooked meat product, whereas the addition of PAW did not influence the CIE L*, a*, b* color values. The use of EF prevented lipid oxidation in cooked meat product during storage, especially the addition of OLE to the EF reduced the TBARS values. In this research, the addition of OLE to EF prepared with PAW has a promising application potential in the preservation of RTE‐cooked meat products. Also, these findings suggested that OLE, a by‐product from production of olive oil, might be applied as active packaging in biodegradable films.
RNA m6A methylation regulatory mechanism of resveratrol in premature senescence cells
Authors: Zhang Xinyu , Zhu Chenyu , Zhang Luyun , Tan Luyi , Cheng Wenli , Li Min , Zhang Xingtan , Zhang Wenjuan , Zhang Wenji 
Published: September 2024

AbstractResveratrol, a natural compound found in various plants, is known for its anti‐inflammatory, antioxidant, and senescence‐delaying properties. RNA N6‐methyladenosine (m6A) methylation plays a crucial role in oxidative stress and premature cellular senescence processes and is closely associated with age‐related disorders. However, the anti‐premature senescence via RNA m6A methylation mechanism of resveratrol is still not fully understood. In this study, based on premature senescence model of human embryonic lung fibroblasts (HEFs) induced by hydrogen peroxide (H2O2), a widely accepted model of premature senescence caused by oxidative stress, we explored the anti‐aging regulatory effects of resveratrol at the RNA m6A methylation level. Our data suggested that resveratrol significantly delayed premature senescence by increasing cell viability, reducing SA‐β‐gal blue staining rate, ROS levels, and senescence‐associated secretory phenotypes (SASP) expression in HEFs. Meanwhile, resveratrol increased the whole RNA methyltransferases activity and the overall m6A level during senescence. Furthermore, three genes CCND2, E2F1, and GADD45B have been identified as the main ones regulating premature by resveratrol. Specifically, it decreased E2F1, GADD45B RNA m6A methylation level, and increased CCND2 level in premature cells. Our study provided new clues for exploring the mechanism and application of resveratrol in the field of premature aging.
Omega‐3 supplementation attenuates doxorubicin‐induced cardiotoxicity but is not related to the ceramide pathway
Authors: Monte Marina Gaiato , Tonon Carolina Rodrigues , Fujimori Anderson Seiji , Ribeiro Ana Paula Dantas , Zanati Silmeia Garcia , Okoshi Katashi , Camacho Camila Renata Correa , Moretto Maria Regina , de Paiva Sergio Alberto Rupp , Zornoff Leonardo Antonio Mamede , Azevedo Paula Schmidt , Minicucci Marcos Ferreira , Polegato Bertha Furlan 
Published: September 2024

AbstractCardiotoxicity is the serious side effect of doxorubicin treatment. Ceramides are formed from the degradation of sphingolipids in cell membranes and play an important role in signaling and modulating biological processes. There is evidence that omega‐3 fatty acid administration can act on this pathway. To evaluate the role of the ceramide pathway in the pathophysiology of doxorubicin‐induced cardiotoxicity and the effect of omega‐3 fatty acid supplementation in the attenuation of chronic doxorubicin‐induced cardiotoxicity in rats. Sixty male Wistar rats were divided into four groups: Control (C), Doxorubicin (D), Omega‐3 fatty acids (W), and Doxorubicin + Omega‐3 fatty acids (DW). The groups received omega‐3 fatty acids (400 mg/kg/day, via gavage) or water for 6 weeks and doxorubicin (3.5 mg/kg, intraperitoneal) or saline once a week for 4 weeks. Doxorubicin‐treated animals showed increases in left atrium and left ventricle diameters, serum triglycerides and cholesterol, malondialdehyde, and protein carbonylation. We also observed a decrease in left ventricular shortening fraction and nSMase1 expression in the heart. Omega‐3 fatty acid supplementation attenuated the structural and functional alterations caused by doxorubicin and decreased protein carbonylation. In contrast to doxorubicin, omega‐3 fatty acids increased neutral nSMase activity in animals that both received and did not receive doxorubicin but with no effect on nSMase1 protein expression. Omega‐3 fatty acid supplementation attenuated the cardiotoxicity caused by doxorubicin. The ceramide pathway may be involved in the pathophysiology of cardiotoxicity, but it is not the mechanism by which omega‐3 fatty acids attenuated cardiac dysfunction.
Microencapsulation and characterization of pomegranate seed oil using gum Arabic and maltodextrin blends for functional food applications
Authors: Mangope Kutloano , Kaseke Tafadzwa , Fawole Olaniyi A. 
Published: September 2024

AbstractPomegranate seed oil (PSO) is highly valued in the functional food industry due to its rich fatty acid content and associated health benefits. However, its high degree of unsaturation makes it susceptible to rapid degradation when exposed to oxygen and light. This study investigates the encapsulation of PSO at 15% w/w using different blends of gum Arabic (GA) and maltodextrin (MD) (1:0, 0:1, 1:1, 3:1, and 1:3) to determine optimal formulations for enhanced stability and functional quality. Characterization of the encapsulated PSO powders showed distinct particle morphologies, including flake‐like shapes and textures ranging from smooth to wrinkled and porous. The Fourier transform infrared (FT‐IR) spectra indicated shifts in functional groups from 2973.70 to 408.84 cm−1, revealing the presence of aliphatic, amine, aromatic, carboxylic acid, and hydroxyl groups. Although no single formulation achieved all desired outcomes, the GA:MD ratios of 1:0 and 1:1 were superior in enhancing color properties (yellowness and chroma), techno‐functional attributes (bulk density and solubility), and in preserving essential fatty acids, including stearic, cis‐oleic, α‐linolenic, arachidic, γ‐linolenic, linoleic, and punicic acids. Additionally, GA:MD (3:1) powders exhibited superior ferric‐reducing antioxidant power (FRAP) and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activities (RSA). In conclusion, formulations using solely GA or GA:MD ratios of 1:1 and 3:1 effectively preserve bioactive content in PSO, enhancing its antioxidant capacity. These findings suggest promising applications for these encapsulated powders in developing functional foods that meet industry demands.
Are Biofuel Technologies a Revolution for Environmental Sustainability in the United States?
Authors: Karlilar Pata Selin , Pata Ugur Korkut 
Published: September 2024

ABSTRACTMany countries accelerate climate change by using fossil fuels like coal, gas, and petroleum to power their energy production systems and boost economic growth, which in turn releases large amounts of greenhouse gases (GHG). Recently, biofuels (BIO) have gained significant global attention for their potential to decarbonize the economy and reduce dependence on petroleum by replacing fossil fuels. This study examines the effect of biofuel consumption and innovation in non‐fossil fuels on the load capacity factor (LCF) in the United States spanning from 1981 to 2020. The empirical outcomes verify the validity of the load capacity curve (LCC) theory. In addition, biofuel consumption has a favourable impact on LCF. The findings further demonstrate that innovation in non‐fossil fuel technologies has no significant impact on the LCF. Based on these outcomes, the US government should increase the share of BIO as an energy component in the energy mix to replace fossil fuels. As a result, BIO have significant potential for the United States to meet its low‐carbon goal by efficiently reducing GHG.
Relationship between advanced glycation end‐products and advanced oxidation protein products in patients with type 2 diabetes with and without albuminuria: A cross‐sectional survey
Authors: Larry Mehrdad , Rabizadeh Soghra , Mohammadi Fatemeh , Yadegar Amirhossein , Jalalpour Azadeh , Mirmiranpour Hossein , Farahmand Ghasem , Esteghamati Alireza , Nakhjavani Manouchehr 
Published: September 2024

AbstractBackground and AimsLiterature suggests that oxidative stress plays a crucial role in the progression of diabetes. Since poor glycemic control enhances the formation of advanced glycation end‐products (AGEs) and advanced oxidation protein products (AOPP) in individuals with diabetes, exploring the association between glycation and oxidative states in diabetes could also shed light on potential consequences. This study evaluated the effects of albuminuria on AGEs and AOPP levels and measured their relationship in participants with type 2 diabetes (T2D) with or without albuminuria.MethodsA cross‐sectional, matched case‐control study was designed, including 38 T2D subjects with albuminuria and 38 matched T2D subjects with normoalbuminuria. Patients were matched by their body mass index (BMI), age, and duration of diabetes. The unadjusted and adjusted correlation between AGEs and AOPP in the studied groups were analyzed by multiple logistic regression. Using ggplot2, the ties between these two biochemical factors in cases and controls were plotted.ResultsThis study elucidated a significant association between AGEs and AOPP in participants with normoalbuminuria (r = 0.331, p‐value < 0.05), which continued to be significant after controlling for BMI, age, systolic blood pressure (SBP), and diastolic blood pressure (DBP) (r = 0.355, p‐value < 0.05). However, there was no significant association between AGEs and AOPP in those with albuminuria in the unadjusted model (r = 0.034, p‐value = 0.841) or after controlling for BMI, age, SBP, and DBP (r = 0.076, p‐value = 0.685).ConclusionOxidation and glycation molecular biomarkers were correlated in patients without albuminuria; however, this association was not observed in those with albuminuria.
Early prediction of gestational diabetes mellitus using first trimester maternal serum pregnancy‐associated plasma protein‐a: A cross‐sectional study
Authors: Amini Maedeh , Kazemnejad Anoshirvan , Rasekhi Aliakbar , Amirian Azam , Kariman Nourossadat 
Published: September 2024

AbstractBackground and AimsThe oral glucose tolerance test with 75 g glucose is commonly regarded as the gold standard (GS) for the detection of gestational diabetes mellitus (GDM). However, one limitation of this test is its administration in the late second trimester of pregnancy in some countries (e.g., Iran). This study aimed to evaluate the accuracy of pregnancy‐associated plasma protein‐A (PAPP‐A) for predicting GDM in the early first trimester of pregnancy using a novel statistical modeling technique.MethodsThe study population consisted of 344 pregnant women who participated in the first trimester screening program for GDM. Maternal serum PAPP‐A levels were measured between 11 and 13 gestational weeks for all participants. A Bayesian latent profile model (LPM) under the skew‐t (ST) distribution was employed to estimate the diagnostic accuracy measures of PAPP‐A in the absence of GS test outcomes.ResultsThe mean (standard deviation) age of the participants was 28.87 ± 5.20 years. The median (interquartile range (IQR)) PAPP‐A MoM was 0.91 (0.69‐1.34). Utilizing the LPM under the ST distribution while adjusting for covariates, the sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) of PAPP‐A were 92% (95% credible interval [CrI]: 0.89, 0.98), 81% (95% CrI: 0.76, 0.91), and 0.91 (95% CrI: 0.83, 0.97), respectively. Notably, the pregnant women with GDM had significantly lower PAPP‐A values ( −0.52, 95% CrI [−0.61, −0.46]).ConclusionGenerally, our findings confirmed that PAPP‐A could serve as a potential screening tool for the identification of GDM in the early stages of pregnancy.
Effects of Blood Collection Tubes on the Quantification of Catecholamines and Their O‐Methylated Metabolites by Liquid Chromatography Coupled With Tandem Mass Spectrometry
Authors: Su Rong , Wu Yanjie , Huang Qiuyi , Huang Shengchun , Li Yao , Chen Caifeng , Xie Wenchun , Li Yan 
Published: September 2024

ABSTRACTBackgroundCatecholamines (epinephrine; norepinephrine; and dopamine) and their O‐methylated metabolites (metanephrine; normetanephrine; and 3‐methoxytyramine) are biomarkers for pheochromocytoma and paraganglioma. Liquid chromatography coupled with tandem mass spectrometry (LC‐MS/MS) was recommended by Endocrine Society for detecting these compounds. The influence of blood collection tubes on the analysis of the six analytes by LC‐MS/MS was not thoroughly investigated, which we want to clarify in our study.MethodsBlood samples of healthy individuals were collected into serum, lithium heparin, and K2EDTA plasma tubes separately. Samples were subjected to solid phase extraction and then analyzed by LC‐MS/MS. The retention behavior and assay performance of the six analytes were assessed for samples from different collection containers. The impacts of potassium and sodium as the counter ions of EDTA on the retention time and matrix effect were also studied.ResultsCompared with O‐methylated metabolites, the results for catecholamines were more affected by the collection tubes, especially for norepinephrine, which displayed severely suppressed signal and very low extraction efficiency in K2EDTA plasma. Changing the counter ion of EDTA from potassium to sodium dramatically changed the retention behavior and matrix effect of norepinephrine.ConclusionsIt is necessary to evaluate blood collection tubes for catecholamines and their O‐methylated metabolites analyzed by LC‐MS/MS. In addition, attention should also be paid when the anticoagulant counter ion was changed.
Intra‐rater and inter‐rater reliability of robotic arthrometer DYNEELAX®
Authors: Mihalinec Katja , Martinez‐Cepa Carmen B. , Zuil‐Escobar Juan C. , Kejžar Nataša , Vauhnik Renata 
Published: September 2024

ABSTRACTPurposeThe purpose of our study was to test the intra‐rater and inter‐rater reliability of the DYNEELAX® arthrometer in healthy subjects. Since rotational laxity will be measured for the first time in humans, indications for normative values will also be presented.MethodsKnee anterior laxity and rotational laxity using a DYNEELAX® arthrometer were assessed in 73 subjects (39 females and 34 males). An intraclass correlation coefficient was calculated to evaluate the intra‐rater and inter‐rater reliability of the DYNEELAX®.ResultsAn intraclass correlation coefficient for intra‐rater reliability ranges from 0.631 (95% confidence interval; [CI]: 0.47–0.75) to 0.699 (95% CI: 0.56–0.80) and from 0.916 (95% CI: 0.87–0.95) to 0.94 (95% CI: 0.91–0.96) for anterior knee laxity and rotational knee laxity, respectively. An intraclass correlation coefficient for inter‐rater reliability ranges from 0.578 (95% CI: 0.40–0.71) to 0.646 (95% CI: 0.44–0.78) and from 0.822 (95% CI: 0.57–0.91) to 0.933 (95% CI: 0.89–0.96) for anterior knee laxity and rotational knee laxity, respectively.ConclusionsThe DYNEELAX® arthrometer has good to excellent intra‐rater and inter‐rater reliability for rotational knee laxity and moderate intra‐rater reliability for anterior knee laxity in healthy subjects. Future studies should investigate the clinical significance of anterior and rotational laxity measured with the DYNEELAX® arthrometer in patients with knee pathology, as both laxities are critical for assessing the integrity of the intra‐articular structures of the knee in clinical practice.Level of evidenceLevel IV.
Anterior bone tunnel position increases meniscus migration in medial meniscus posterior root repair: A cadaveric study of suture length changes
Authors: Nishino Kazuya , Hashimoto Yusuke , Kinoshita Takuya , Iida Ken , Tsumoto Shuko , Nakamura Hiroaki 
Published: September 2024

AbstractPurposeThis study investigated differences in the migration of meniscus sutured with pull‐out sutures for treating medial meniscus posterior root tears (MMPRTs) according to the bone tunnel position, using cadaveric knees.MethodsSix knees of three donors fixed using Thiel's method were included in this study. The MMPRTs were created, and a single suture was performed at the torn meniscus using an arthroscopic procedure. The suture was pulled out through the tibial bone tunnel, and the meniscus displacement was measured as the change in length during 0–120° of knee flexion. Three types of bone tunnels (anatomical, anterior and posterior) were created for each knee, and the sutures were pulled out of each tunnel three times. After completing all measurements, the proximal tibia was extracted and micro‐computed tomography was performed to evaluate the tunnel position.ResultsA significantly smaller change in suture length was observed in the posterior group compared to the other two groups (anatomical group, 5.17 ± 1.8 mm; anterior group, 7.50 ± 3.2 mm; posterior group, 1.17 ± 1.0 mm; p > 0.01). In addition, a significant correlation between the anteroposterior tunnel position and suture length change was observed (r = −0.720; p = 0.001).ConclusionsWhen pull‐out sutures were used to repair MMPRTs, the suture length change was approximately 5 mm during knee flexion and extension when the bone tunnel was located at the anatomical attachment site. This change was larger when the tunnel position was anterior, and smaller when the tunnel position was posterior.Level of EvidenceLEVEL Ⅲ case–control study
Non‐Hamiltonian Cartesian products of two even dicycles
Authors: Noguchi Kenta , Zamfirescu Carol T. 
Published: September 2024

AbstractIn this note it is proven that there exist infinitely many positive integers and such that the Cartesian product of a directed cycle of length and a directed cycle of length is non‐Hamiltonian. In particular, the Cartesian product of an 880‐dicycle and a 4368‐dicycle is non‐Hamiltonian. We also prove that there is no such graph on fewer than vertices, which is rather astonishing.
Trade‐offs between labour migration and agricultural productivity: Evidence from smallholder wheat systems in Nepal
Authors: Paudel Gokul P. , Nguyen Trung Thanh , Grote Ulrike 
Published: September 2024

AbstractRural labour out‐migration has become a major contributor to off‐farm income through remittances and plays a crucial role in supporting the livelihoods of rural households in developing economies. However, research on the simultaneous on‐farm and off‐farm impacts of labour migration is still lacking. This study assesses the impacts of household labour migration on wheat productivity, labour and total costs, profitability and off‐farm income among smallholder wheat growers in Nepal. We use endogenous switching regression and two‐stage least squares regression models to control for potential endogeneity. The findings reveal that labour migration boosts off‐farm income due to remittances but negatively affects wheat productivity and profitability due to labour shortages. In addition, heterogeneous effects are observed, with large farms, cooperative membership, use of farm mechanization and non‐marginalized castes recording positive impacts. The study suggests that social institutions, such as cooperatives, and farm mechanization can create synergies between labour migration and agricultural productivity in Nepal.
Century‐Long Variations in Surface Incident Solar Radiation Over Japan——Characterized by Observations and Reanalyses
Authors: Ma Qian , Liu Han 
Published: September 2024

ABSTRACTSurface incident solar radiation (R

s
) is of great importance in determining the energy balance in the Earth system. In this study, we use century‐long homogenized observations over Japan to constrain five 20th century reanalyses to explore their performance in reproducing R

s
variation on different time scales (high‐frequency components [HFCs], for signals with cycles less than 10 years; low‐frequency components [LFCs], for signals with cycles more than 10 years) by ensemble empirical mode decomposition (EEMD) method and to quantify the impact factors on the R

s
estimations by the sum of tree (SOT) model. It is found that ERA20C, ERA20CM and CERA20C overestimated R

s
by 0.48–1.49 W/m−2, while 20CRv2c and 20CRv3 underestimated R

s
by −0.63 and −1.18 W/m−2, respectively for 1931–2010. Poor correlation coefficient (R) was found to be 0.10 for ERA20CM and 0.22 for 20CRv2c. 20CRv2c failed for 1931–1960 but improved considerably for 1961–2010. 20CRv3 uses an upgraded model and assimilates more observations compared with its predecessor 20CRv2c; however, only the original components and HFCs in R

s
were improved, with nearly no improvement in the LFCs. In general, CERA20C R

s
, with small biases and higher R of 0.73 for original signals, 0.83 for HFCs and LFCs, is superior to other reanalyses. No obvious trend in clear sky R

s
demonstrated that reanalysed R

s
are insensitive to the aerosol forcings. Therefore, cloud cover and water vapour maybe the main factors influenced reanalysed R

s
. Most of time, R

s
is more sensitive to cloud cover than vapour pressure for all reanalyses except original signals (with contribution ratio of 0.29 for cloud cover and 0.71 for vapour pressure) and LFCs (with contribution ratio of 0.41 for cloud cover and 0.59 for vapour pressure) in CERA20C, and original signals (with contribution ratio of 0.37 for cloud cover and 0.63 for vapour pressure) in ERA20C. This work pointed out that aerosol related processes such as aerosol forcings or aerosol radiative effect in reanalyses should be improved in the future, which will ultimately improve the interaction between aerosol and cloud in R

s
simulations.
Region‐selective shift register by a single output enable signal for a multiple refresh frequency driving scheme
Authors: Chang Min Kyu , Kim Ji Hoon , Nam Hyoungsik 
Published: September 2024

AbstractMany low‐power technologies have been developed for high‐performance displays. One method is to vary the refresh rate according to the input image, where frequencies are reduced over still images and slow‐moving videos. To support the variable refresh rate scheme, the programmable gate driver circuit should be integrated. This paper proposes a thin‐film transistor (TFT) shift register of which output pulse is simply controlled by an enable signal. Because the output pulse generation is directly adjusted without the previous programming period, various output pulse sequences are possible with the high flexibility. The shift register is composed by adding six TFTs and one capacitor to a conventional circuit that provides a carry pulse to a next stage. The simulation program with integrated circuit emphasis (SPICE) simulation ensures that gate pulses for any numbers of lines in any regions can be generated within one frame only by applying the enable signal at the corresponding timing, resulting in multiple refresh frequencies in a screen. In addition, it is verified that additional TFTs and capacitor contribute to only the negligible increase in power consumption by 2.1%, compared to a conventional shift register.