bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflammatory Osteoclasts Prime TNFα-Producing CD4+ T Cells and Express CX3CR1
Authors: Ibáñez Lidia , Abou-Ezzi Grazia , Ciucci Thomas , Amiot Vanessa , Belaïd Nourhène , Obino Dorian , Mansour Anna , Rouleau Matthieu , Wakkach Abdelilah , Blin-Wakkach Claudine 
Published: May 2016

ABSTRACT

Bone destruction is a hallmark of chronic rheumatic diseases. Although the role of osteoclasts in bone loss is clearly established, their implication in the inflammatory response has not been investigated despite their monocytic origin. Moreover, specific markers are lacking to characterize osteoclasts generated in inflammatory conditions. Here, we have explored the phenotype of inflammatory osteoclasts and their effect on CD4+ T cell responses in the context of bone destruction associated with inflammatory bowel disease. We used the well-characterized model of colitis induced by transfer of naive CD4+ T cells into Rag1–/– mice, which is associated with severe bone destruction. We set up a novel procedure to sort pure osteoclasts generated in vitro to analyze their phenotype and specific immune responses by FACS and qPCR. We demonstrated that osteoclasts generated from colitic mice induced the emergence of TNFα-producing CD4+ T cells, whereas those generated from healthy mice induced CD4+FoxP3+ regulatory T cells, in an antigen-dependent manner. This difference is related to the osteoclast origin from monocytes or dendritic cells, to their cytokine expression pattern, and their environment. We identified CX3CR1 as a marker of inflammatory osteoclasts and we demonstrated that the differentiation of CX3CR1+ osteoclasts is controlled by IL-17 in vitro. This work is the first demonstration that, in addition to participating to bone destruction, osteoclasts also induce immunogenic CD4+ T cell responses upon inflammation. They highlight CX3CR1 as a novel dual target for antiresorptive and anti-inflammatory treatment in inflammatory chronic diseases. © 2016 American Society for Bone and Mineral Research.
TP53 Mutations in Head and Neck Squamous Cell Carcinoma and Their Impact on Disease Progression and Treatment Response
Authors: Zhou Ge , Liu Zhiyi , Myers Jeffrey N. 
Published: May 2016

ABSTRACTRecent studies describing the mutational landscape of head and neck squamous cell carcinoma (HNSCC) on a genomic scale by our group and others, including The Cancer Genome Atlas, have provided unprecedented perspective for understanding the molecular pathogenesis of HNSCC progression and response to treatment. These studies confirmed that mutations of the TP53 tumor suppressor gene were the most frequent of all somatic genomic alterations in HNSCC, alluding to the importance of the TP53 gene in suppressing the development and progression of this disease. Clinically, TP53 mutations are significantly associated with short survival time and tumor resistance to radiotherapy and chemotherapy in HNSCC patients, which makes the TP53 mutation status a potentially useful molecular factor for risk stratification and predictor of clinical response in these patients. In addition to loss of wild‐type p53 function and the dominant‐negative effect on the remaining wild‐type p53, some p53 mutants often gain oncogenic functions to promote tumorigenesis and progression. Different p53 mutants may possess different gain‐of‐function properties. Herein, we review the most up‐to‐date information about TP53 mutations available via The Cancer Genome Atlas‐based analysis of HNSCC and discuss our current understanding of the potential tumor‐suppressive role of p53, focusing on gain‐of‐function activities of p53 mutations. We also summarize our knowledge regarding the use of the TP53 mutation status as a potential evaluation or stratification biomarker for prognosis and a predictor of clinical response to radiotherapy and chemotherapy in HNSCC patients. Finally, we discuss possible strategies for targeting HNSCCs bearing TP53 mutations. J. Cell. Biochem. 117: 2682–2692, 2016. © 2016 Wiley Periodicals, Inc.
Molecular Profiling and Malignant Behavior Define Two Rat Mammary Tumor Cell Lines as a Relevant Experimental Model
Authors: Vela Elena , Escrich Eduard 
Published: May 2016

ABSTRACTCancer cell lines have become a reliable tool in genetic and biochemical studies of breast cancer. Here, we described the behavior and novel molecular characterization of two cell lines derived from DMBA‐induced rat mammary tumor, LA7 and RBA. LA7 cells have been identified as myoepithelial cells with stem cell properties, whereas the RBA cell line are epithelial cells that present mutational activated H‐Ras, but are much less known. We evaluated the proliferation rate and molecular markers, several signaling pathways status related to proliferation, survival, inflammation, and apoptosis, as well as migration capacity, global DNA methylation levels, and stem cells populations. In fact, we found the A/T transversion in the c‐Ha‐Ras codon 61 as the activator mutation origin described in RBA cells. LA7 and RBA cells showed a high proliferation rate associated with overexpression of Cyclin D1, and resistance to apoptotic signals due to lack of expression of Bad. Moreover, neither of these two cell lines expressed steroid receptors, but they showed high migration capacity, all in accordance with an aggressive phenotype. We found global DNA methylation levels in LA7 and RBA cells lower than reference tissues analyzed, in addition to the presence of different stem cells populations in RBA cell line that differed in the expression of CD44 and CD24. These results revealed a malignant behavior associated with cancer stem cell phenotype. Since this profile is similar to a human triple‐negative basal‐like tumor, their extensive characterization presented herein increases their value as a good in vitro model. J. Cell. Biochem. 117: 2825–2834, 2016. © 2016 Wiley Periodicals, Inc.
The ERK1/2 and mTORC1 Signaling Pathways Are Involved in the Muscarinic Acetylcholine Receptor‐Mediated Proliferation of SNU‐407 Colon Cancer Cells
Authors: Park Yang‐Seo , Liu Ziyu , Vasamsetti Bala Murali Krishna , Cho Nam Jeong 
Published: May 2016

ABSTRACTMuscarinic acetylcholine receptors (mAChRs) regulate diverse cellular functions, including cell growth and proliferation, via multiple signaling pathways. Previously, we showed that mAChRs stimulate the MEK1/2‐ERK1/2‐RSK pathway in SNU‐407 colon cancer cells and subsequently promote cell proliferation. In this study, we provide evidence that the PI3K‐Akt‐mTORC1‐S6K1 pathway is activated by mAChRs in SNU‐407 cells and that this pathway is associated with protein biosynthesis and cell proliferation. When the cells were treated with the cholinergic agonist carbachol, Akt was activated in a dose‐ and time‐dependent fashion. This carbachol effect was almost completely blocked by the PI3K inhibitor LY294002, implying that PI3K is responsible for the Akt activation. S6K1, a major downstream target of mTORC1, was also activated by carbachol in a temporal profile similar to that of the Akt activation. This carbachol‐stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K‐Akt‐mTORC1 pathway. We observed that global protein biosynthesis, monitored by puromycin incorporation, was strongly increased by carbachol in an atropine‐sensitive manner. Inhibition experiments indicated that the ERK1/2 and mTORC1 signaling pathways may be involved in carbachol‐stimulated global protein biosynthesis. We also found that treating SNU‐407 cells with LY294002 or rapamycin significantly suppressed carbachol‐stimulated cell proliferation. In the presence of the MEK1/2 inhibitor U0126, cell proliferation was further reduced by rapamycin treatment. Our data thus suggest that both the MEK1/2‐ERK1/2 and mTORC1 pathways play important roles in mAChR‐mediated cell proliferation in SNU‐407 colon cancer cells. J. Cell. Biochem. 117: 2854–2863, 2016. © 2016 Wiley Periodicals, Inc.
Synthesis of zeolitic imidazolate frameworks (ZIF)‐8 membrane and its process optimization study in separation ofCO2from natural gas
Authors: Lai Li Sze , Yeong Yin Fong , Lau Kok Keong , Shariff Azmi Mohd 
Published: May 2016

ABSTRACTBACKGROUNDIn this work, well‐intergrown zeolitic imidazolate frameworks‐8 (ZIF‐8) membrane was synthesized through a microwave‐assisted secondary solvothermal growth method. A solvent evaporation seeding method was applied prior to membrane growth. Subsequently, process optimization for the separation ofCO2from natural gas through theZIF‐8 membrane was studied using response surface methodology. The process variables studied includedCO2feed composition (36–83%), total pressure difference (100–700kPa) and temperature (303–343 K). Three empirical models were generated using a central composite design in order to study the interaction between the process variables and the responses, includingCO2permeance,CH4permeance andCO2/CH4separation factor. The optimum condition was then determined for maximumCO2permeance andCO2/CH4separation factor and minimumCH4permeance.RESULTSUsing this method,ZIF‐8 seeds of nano‐size were well distributed on the support due to high nucleation rate at the supersaturation condition. The presence of heavy hydrocarbons affectedCH4permeance, attributed to the molar mass and viscosity. An optimumCO2permeance of 2.76 × 10−8mol m−2s−1Pa−1andCO2/CH4separation factor of 3.72 was obtained at 312 K, 100kPaand 36%CO2feed composition. The experimental results were in agreement with the predicted values with an average error lower than ± 7%.CONCLUSIONSelectiveZIF‐8 membrane was successfully synthesized using a feasible and reproducible solvent evaporation seeding method. The experimental data for the optimum condition were in good agreement with the predicted data, showing the accuracy of the empirical models. © 2016 Society of Chemical Industry
A qualitative analysis of patients' experience with hospitalist service handovers
Authors: Wray Charlie M. , Farnan Jeanne M. , Arora Vineet M. , Meltzer David O. 
Published: May 2016

BACKGROUNDInpatient service handoffs occur when physicians who care for hospitalized patients end a period of clinical service and handover a panel of patients to an oncoming physician. Despite the large amount of research on handoffs, none has described the patient perspective when cared for by a hospitalist physician during a service handoff.OBJECTIVETo describe hospitalized patients' experiences regarding inpatient service changes, and develop a conceptual framework to inform future efforts to improve service‐level handoffs.METHODSInterview‐based qualitative analysis using in‐depth, semistructured interviews of hospitalized patients on a nonteaching hospitalist service. Patients were interviewed between October 2014 and December 2014 at an academic medical center whose inpatient stay spanned a weekly service change. We utilized an inductive approach with no a priori hypotheses and used a constant comparative method to generate emerging themes to develop a conceptual model that captured the patient experience during the transition.RESULTSOf patients who agreed to participate (40/43), most (85%) were unaware that a transition had occurred between their hospitalists. Six major themes emerged related to patients' experiences with hospitalist service handoffs: (1) importance of physician‐patient communication, (2) desire for transparency in transitions, (3) an indifference toward transitions, (4) importance of hospitalist‐specialist communication, (5) formation of new opportunities from a transition, and (6) effects of bedside manner.CONCLUSIONSHospitalized patients desire improved communication and a more formalized transition process between hospitalists during service handoffs. Hospitalists should recognize that this transition may represent an opportunity to improve the hospitalized patient's experience and satisfaction. Journal of Hospital Medicine 2016;11:675–681. © 2016 Society of Hospital Medicine
Simultaneous qualitative and quantitative analysis of flavonoids and alkaloids from the leaves of Nelumbo nucifera Gaertn. using high‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry
Authors: Guo Yujie , Chen Xi , Qi Jin , Yu Boyang 
Published: May 2016

A reliable method, combining qualitative analysis by high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry and quantitative assessment by high‐performance liquid chromatography with photodiode array detection, has been developed to simultaneously analyze flavonoids and alkaloids in lotus leaf extracts. In the qualitative analysis, a total of 30 compounds, including 12 flavonoids, 16 alkaloids, and two proanthocyanidins, were identified. The fragmentation behaviors of four types of flavone glycoside and three types of alkaloid are summarized. The mass spectra of four representative components, quercetin 3‐O‐glucuronide, norcoclaurine, nuciferine, and neferine, are shown to illustrate their fragmentation pathways. Five pairs of isomers were detected and three of them were distinguished by comparing the elution order with reference substances and the mass spectrometry data with reported data. In the quantitative analysis, 30 lotus leaf samples from different regions were analyzed to investigate the proportion of eight representative compounds. Quercetin 3‐O‐glucuronide was found to be the predominant constituent of lotus leaf extracts. For further discrimination among the samples, hierarchical cluster analysis, and principal component analysis, based on the areas of the eight quantitative peaks, were carried out.
Directional fluorescence emission from a compact plasmonic‐diamond hybrid nanostructure
Authors: Shen Hongming , Chou R. Y. , Hui Yuen Yung , He Yingbo , Cheng Yuqing , Chang Huan‐Cheng , Tong Limin , Gong Qihuang , Lu Guowei 
Published: May 2016

AbstractUltracompact directional optical nanoantennas are rapidly gaining popularity yet still challenging tasks to construct at the nanoscale. Here, we experimentally demonstrate unidirectional emission from a fluorescent nanodiamond coupled with a single gold nanorod. Different configurations of the assembled hybrid nanostructures were realized via step‐by‐step atomic force microscope nanomanipulation. The emission patterns can be controlled by adjusting the configurations, i.e., the gold nanorod orientation and separation with respect to the nanodiamond. Numerical simulation results reveal that the unidirectional emission can be ascribed to the interference between the electromagnetic fields produced by the dipole‐like source and the out‐of‐phase dipole induced in the gold nanorod. The proposed hybrid nanostructures remarkably exhibit highly unidirectional emission even when the emitter is positioned up to 50 nm away from the nanorod antenna and present a broad working spectral bandwidth of ∼200 nm. The distinct features of this hybrid structure suggest potential applications for novel nanoscale light sources, sensing, and quantum information.
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Stereocomplex Crystallization of Star‐Shaped 4‐Armed Equimolar Stereo Diblock Poly(lactide)s with Different Molecular Weights: Isothermal Crystallization from the Melt
Authors: Tsuji Hideto , Matsumura Nobutsugu 
Published: May 2016

The isothermal crystallization of star‐shaped four‐armed equimolar stereo diblock poly(lactide) (4‐LD) polymers with different molecular weights is investigated. Solely stereocomplex (SC) crystallites are formed in all equimolar 4‐LD polymers, irrespective of molecular weight and crystallization temperature (Tc). The wide‐angle X‐ray diffractometry, differential scanning calorimetry, and polarized optical microscopy results for crystalline species, crystallinity, and maximum radial growth rate of spherulites values indicate that both branching and diblock architectures disturb the SC crystallization and spherulites growth of equimolar 4‐LD polymers, and the disturbance effect is larger for branching architecture than for diblock architecture. The equilibrium melting temperature (Tm0) values are 181.9–266.0 °C, which are comparable with or lower than the value reported earlier (279 °C). The crystallite growth geometries of equimolar of 4‐LD polymers are independent of molecular weight and Tc.
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Being True to Oneself: Investigating Celebrity Brand Authenticity
Authors: Ilicic Jasmina , Webster Cynthia M. 
Published: May 2016

ABSTRACTCelebrity brand authenticity is introduced as a construct that represents consumer perceptions of celebrities being “true to oneself” in their behaviors and interactions with consumers. A scale is developed through two purification stages and the scale's predictive validity is assessed. First, the meaning of celebrity brand authenticity to consumers is explored. Second, the Authenticity Inventory from the psychology literature is adapted to develop a scale for consumer perceptions of celebrity brand authenticity. Celebrity brands are perceived as true to self when they appear genuine in their relationships with consumers and behave in accordance with their perceived held values. Evidence of the convergent and discriminant validity of the celebrity brand authenticity scale is provided, which confirms celebrity brand authenticity as distinct from celebrity attachment, despite containing relational items. Finally, the predictive power of celebrity brand authenticity is confirmed through positively influencing consumer intentions to purchase an endorsed brand. Brand managers can use celebrity brand authenticity to position or develop celebrity brands, as well as in the selection of celebrity endorsers.
Surgical readmissions: results of integrating pre‐, peri‐ and postsurgical care
Authors: Noyes Katia , Baack‐Kukreja Janet , Messing Edward M. , Schoeniger Luke , Galka Eva , Pan Wei , Xueya Cai , Fleming Fergal J. , Monson John RT , Mohile Supriya G. , Francone Todd 
Published: May 2016

AbstractAimsTo explore the feasibility of recruiting surgical oncology patients and implementing a surgical integrated discharge (SID) programme led by advanced practice providers (APP).BackgroundBurden of illness and complexity of treatment regimen makes it challenging for surgical oncology patients to participate in research. Surgical oncology nurses may have the necessary expertise to overcome this problem.DesignControlled longitudinal prospective observational study.MethodsThe SID programme included multidisciplinary care coordination, regular communication among APPs and proactive postdischarge follow‐up. Administrative databases were used to identify matching historical controls (n = 113) and evaluate programme outcomes.ResultsPatient enrolment was 84%. The main challenges for the programme implementation included incompatible health information systems among care settings, variation in care processes among hospital units and need for provider behaviour change.ConclusionsMost surgical oncology patients are willing to participate in outcomes programmes when contacted by familiar clinical personnel but programme implementation requires leadership support, communication among care teams and training and infrastructure.
Spectral study and classification of worldwide locations considering several multijunction solar cell technologies
Authors: Núñez Rubén , Jin Chen , Victoria Marta , Domínguez César , Askins Stephen , Herrero Rebeca , Antón Ignacio , Sala Gabriel 
Published: May 2016

AbstractMulti‐junction solar cells are widely used in high‐concentration photovoltaic systems (HCPV) attaining the highest efficiencies in photovoltaic energy generation. This technology is more dependent on the spectral variations of the impinging Direct Normal Irradiance (DNI) than conventional photovoltaics based on silicon solar cells and consequently demands a deeper knowledge of the solar resource characteristics. This article explores the capabilities of spectral indexes, namely, spectral matching ratios (SMR), to spectrally characterize the annual irradiation reaching a particular location on the Earth and to provide the necessary information for the spectral optimization of a MJ solar cell in that location as a starting point for CPV module spectral tuning. Additionally, the relationship between such indexes and the atmosphere parameters, such as the aerosol optical depth (AOD), precipitable water (PW), and air mass (AM), is discussed using radiative transfer models such as SMARTS to generate the spectrally resolved DNI. The network of ground‐based sun and sky‐scanning radiometers AErosol RObotic NETwork (AERONET) is exploited to obtain the atmosphere parameters for a selected bunch of 34 sites worldwide. Finally, the SMR indexes are obtained for every location, and a comparative analysis is carried out for four architectures of triple junction solar cells, covering both lattice match and metamorphic technologies. The differences found among cell technologies are much less significant than among locations. Copyright © 2016 John Wiley & Sons, Ltd.
Effect of circular permutations on transient partial unfolding in proteins
Authors: Chen Chen , Yun Jung‐Hun , Kim Jae‐Hoon , Park Chiwook 
Published: May 2016

AbstractUnder native conditions, proteins can undergo transient partial unfolding, which may cause proteins to misfold or aggregate. A change in sequence connectivity by circular permutation may affect the energetics of transient partial unfolding in proteins without altering the three‐dimensional structures. Using Escherichia coli dihydrofolate reductase (DHFR) as a model system, we investigated how circular permutation affects transient partial unfolding in proteins. We constructed three circular permutants, CP18, CP37, and CP87, with the new N‐termini at residue 18, 37, and 87, respectively, and probed transient partial unfolding by native‐state proteolysis. The new termini in CP18, CP37, and CP87 are within, near, and distal to the Met20 loop, which is known to be dynamic and also part of the region that undergoes transient unfolding in wild‐type DHFR. The stabilities of both native and partially unfolded forms of CP18 are similar to those of wild‐type DHFR, suggesting that the influence of introducing new termini in a dynamic region to the protein is minimal. CP37 has a significantly more accessible partially unfolded form than wild‐type DHFR, demonstrating that introducing new termini near a dynamic region may promote transient partial unfolding. CP87 has significantly destabilized native and partially unfolded forms, confirming that modification of the folded region in a partially unfolded form destabilizes the partially unfolded form similar to the native form. Our findings provide valuable guidelines to control transient partial unfolding in designing circular permutants in proteins.