bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid method for quantifying cytoplasmic versus nuclear localization in endogenous peripheral blood leukocytes by conventional flow cytometry
Authors: Brittain George C. , Gulnik Sergei 
Published: March 2017

AbstractA biochemical system and method have been developed to enable the quantitative measurement of cytoplasmic versus nuclear localization within cells in whole blood. Compared with the analyses of nuclear localization by western blot or fluorescence microscopy, this system saves a lot of time and resources by eliminating the necessity of purification and culturing steps, and generates data that are free from the errors and artifacts associated with using tumor cell lines or calculating nuclear signals from 2D images. This user‐friendly system enables the analysis of cell signaling within peripheral blood cells in their endogenous environment, including measuring the kinetics of nuclear translocation for transcription factors without requiring protein modifications. We first demonstrated the efficiency and specificity of this system for targeting nuclear epitopes, and verified the results by fluorescence microscopy. Next, the power of the technique to analyze LPS‐induced signaling in peripheral blood monocytes was demonstrated. Finally, both FoxP3 localization and IL‐2‐induced STAT5 signaling in regulatory T cells were analyzed. We conclude that this system can be a useful tool for enabling multidimensional molecular‐biological analyses of cell signaling within endogenous peripheral blood cells by conventional flow cytometry. © 2017 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.
Plasticity results in delayed breeding in a long‐distant migrant seabird
Authors: Dobson F. Stephen , Becker Peter H. , Arnaud Coline M. , Bouwhuis Sandra , Charmantier Anne 
Published: March 2017

AbstractA major question for conservationists and evolutionary biologists is whether natural populations can adapt to rapid environmental change through micro‐evolution or phenotypic plasticity. Making use of 17 years of data from a colony of a long‐distant migratory seabird, the common tern (Sterna hirundo), we examined phenotypic plasticity and the evolutionary potential of breeding phenology, a key reproductive trait. We found that laying date was strongly heritable (0.27 ± 0.09) and under significant fecundity selection for earlier laying. Paradoxically, and in contrast to patterns observed in most songbird populations, laying date became delayed over the study period, by about 5 days. The discrepancy between the observed changes and those predicted from selection on laying date was explained by substantial phenotypic plasticity. The plastic response in laying date did not vary significantly among individuals. Exploration of climatic factors showed individual responses to the mean sea surface temperature in Senegal in December prior to breeding: Common terns laid later following warmer winters in Senegal. For each 1°C of warming of the sea surface in Senegal, common terns delayed their laying date in northern Germany by 6.7 days. This suggests that warmer waters provide poorer wintering resources. We therefore found that substantial plastic response to wintering conditions can oppose natural selection, perhaps constraining adaptation.
Testing the role of ancient and contemporary landscapes on structuring genetic variation in a specialist grasshopper
Authors: Noguerales Víctor , Cordero Pedro J. , Ortego Joaquín 
Published: March 2017

AbstractUnderstanding the processes underlying spatial patterns of genetic diversity and structure of natural populations is a central topic in evolutionary biogeography. In this study, we combine data on ancient and contemporary landscape composition to get a comprehensive view of the factors shaping genetic variation across the populations of the scrub‐legume grasshopper (Chorthippus binotatus binotatus) from the biogeographically complex region of southeast Iberia. First, we examined geographical patterns of genetic structure and employed an approximate Bayesian computation (ABC) approach to compare different plausible scenarios of population divergence. Second, we used a landscape genetic framework to test for the effects of (1) Late Miocene paleogeography, (2) Pleistocene climate fluctuations, and (3) contemporary topographic complexity on the spatial patterns of population genetic differentiation. Genetic structure and ABC analyses supported the presence of three genetic clusters and a sequential west‐to‐east splitting model that predated the last glacial maximum (LGM, c. 21 Kya). Landscape genetic analyses revealed that population genetic differentiation was primarily shaped by contemporary topographic complexity, but was not explained by any paleogeographic scenario or resistance distances based on climate suitability in the present or during the LGM. Overall, this study emphasizes the need of integrating information on ancient and contemporary landscape composition to get a comprehensive view of their relative importance to explain spatial patterns of genetic variation in organisms inhabiting regions with complex biogeographical histories.
Median floor acceleration spectra of linear structures with uncertain properties
Authors: Lucchini Andrea , Franchin Paolo , Mollaioli Fabrizio 
Published: March 2017

SummaryResponse parameters used to estimate nonstructural damage differ depending on whether deformation‐sensitive or acceleration‐sensitive components are considered. In the latter case, seismic demand is usually represented through floor spectra, that is response spectra in terms of pseudo‐acceleration, which are calculated at the floor levels of the structure where the nonstructural components are attached to. Objective of this paper is to present a new spectrum‐to‐spectrum method for calculating floor acceleration spectra, which is able to explicitly account for epistemic uncertainties in the modal properties of the supporting structure. By using this method, effects on the spectra of possible variations from nominal values of the periods of vibration of the structure can be estimated. The method derives from the extension of closed‐form equations recently proposed by the authors to predict uniform hazard floor acceleration spectra. These equations are built to rigorously account for the input ground motion uncertainty, that is the record‐to‐record variability of the nonstructural response. In order to evaluate the proposed method, comparisons with exact spectra obtained from a standard probabilistic seismic demand analysis, as well as spectra calculated using the Eurocode 8 equation, are finally shown. Copyright © 2017 John Wiley & Sons, Ltd.
Conceptualizing the Use of Translanguaging in Initial Content Assessments for Newly Arrived Emergent Bilingual Students
Authors: Lopez Alexis A. , Turkan Sultan , Guzman‐Orth Danielle 
Published: March 2017

AbstractTranslanguaging refers to the flexible use of the bilingual repertoire. In this report, we provide a theoretical framework to support the use of translanguaging to assess the academic content knowledge of newly arrived emergent bilingual students. In this report, we argue that translanguaging offers newly arrived emergent bilingual students the opportunity to demonstrate what they know and can do even if their English language and literacy skills are not fully developed. We propose two principles for incorporating translanguaging into initial content assessments for late‐arriving emergent bilingual students, and we theorize how translanguaging can be used within an assessment context by discussing test design applications and considerations. We also discuss some challenges when incorporating translanguaging within an assessment context.
Higher serum cholesterol is associated with intensified age‐related neural network decoupling and cognitive decline in early‐ to mid‐life
Authors: Spielberg Jeffrey M. , Sadeh Naomi , Leritz Elizabeth C. , McGlinchey Regina E. , Milberg William P. , Hayes Jasmeet P. , Salat David H. 
Published: March 2017

AbstractMounting evidence indicates that serum cholesterol and other risk factors for cardiovascular disease intensify normative trajectories of age‐related cognitive decline. However, the neural mechanisms by which this occurs remain largely unknown. To understand the impact of cholesterol on brain networks, we applied graph theory to resting‐state fMRI in a large sample of early‐ to mid‐life Veterans (N = 206, Meanage = 32). A network emerged (centered on the banks of the superior temporal sulcus) that evidenced age‐related decoupling (i.e., decreased network connectivity with age), but only in participants with clinically‐elevated total cholesterol (≥180 mg/dL). Crucially, decoupling in this network corresponded to greater day‐to‐day disability and mediated age‐related declines in psychomotor speed. Finally, examination of network organization revealed a pattern of age‐related dedifferentiation for the banks of the superior temporal sulcus, again present only with higher cholesterol. More specifically, age was related to decreasing within‐module communication (indexed by Within‐Module Degree Z‐Score) and increasing between‐module communication (indexed by Participation Coefficient), but only in participants with clinically‐elevated cholesterol. Follow‐up analyses indicated that all findings were driven by low‐density lipoprotein (LDL) levels, rather than high‐density lipoprotein (HDL) or triglycerides, which is interesting as LDL levels have been linked to increased risk for cardiovascular disease, whereas HDL levels appear inversely related to such disease. These findings provide novel insight into the deleterious effects of cholesterol on brain health and suggest that cholesterol accelerates the impact of age on neural trajectories by disrupting connectivity in circuits implicated in integrative processes and behavioral control. Hum Brain Mapp 38:3249–3261, 2017. © 2017 Wiley Periodicals, Inc.
European hydroclimate response to volcanic eruptions over the past nine centuries
Authors: Gao Yujuan , Gao Chaochao 
Published: March 2017

ABSTRACTThe climatic aftermath of the 1815 Tambora eruption in Europe suggests that large volcanic eruptions can introduce environmental and societal consequences in this region. Here, we analyse the European summer hydrological response to 31 tropical and 44 Northern Hemisphere mid‐to‐high latitude eruptions over the past nine centuries, using a newly published reconstruction of global volcanism and a proxy record of hydrological conditions (Old World Drought Atlas) together with a superposed epoch analysis. Our results show a significant wetting response (at the 95% confidence level) for year 0 and year +1 after tropical eruptions, followed by a significant drying in year +2. Spatially, wetting occurs in northeast and southern Europe, while a drying response develops in central and northwest Europe. Both the wetting and drying responses are more significant for the group of eruptions with higher sulphate injection magnitudes than the eruptions with low magnitudes. Large high latitude eruptions tend to cause a drying response in western‐central Europe in year +2, which shifts south‐eastwards in years +3 and +4. Correcting for the effects of El Nino does not noticeably change the response patterns. The response to eruptions counteracts the hydroclimate response to global warming in some regions, e.g. the Mediterranean, but exaggerates is in others, such as central Europe. Our results verify previous modelling studies from a longer term proxy perspective, and illuminate potential effects of stratospheric geoengineering in Europe.
Prediction of viral loads for diagnosis of Hepatitis C infection in human plasma samples using Raman spectroscopy coupled with partial least squares regression analysis
Authors: Nawaz H. , Rashid N. , Saleem M. , Asif Hanif M. , Irfan Majeed M. , Amin I. , Iqbal M. , Rahman M. , Ibrahim Ola , Baig S. M. , Ahmed M. , Bonnier F. , Byrne H. J. 
Published: March 2017

Raman spectroscopy has been used to identify the biochemical changes associated with the presence of the Hepatitis C virus (HCV) in infected human blood plasma samples as compared with healthy samples, as control. The aim of the study was to establish the Raman spectral markers of hepatitis infection, which could be used for diagnostic purposes. Moreover, multivariate data analysis techniques, including Principal Component Analysis (PCA), coupled with Linear Discriminant Analysis (LDA), and Partial Least Square Regression (PLSR) are employed to further demonstrate the diagnostic capability of the technique. The PLSR model is developed to predict the viral loads of the HCV infected plasma on the basis of the biochemical changes caused by the viral infection.Specific Raman spectral features are observed in the mean spectra of HCV plasma samples which are not observed in the control mean spectra. PCA differentiated the ‘normal’ and ‘HCV’ groups of the Raman spectra and PCA‐LDA was employed to increase the efficiency of prediction of the presence of HCV infection, resulting in a sensitivity and specificity 98.8% and 98.6%, with corresponding Positive Predictive Value of 99.2%, and Negative Predictive Value of 98%. PLSR modelling was found to be 99% accurate in predicting the actual viral loads of the HCV samples, as determined clinically using the Polymerase Chain Reaction (PCR) technique, on the basis of the Raman spectral changes caused by the virus during the process of the development of Hepatitis C. Copyright © 2017 John Wiley & Sons, Ltd.
Sequence‐Defined Oligoamide Drug Conjugates of Pretubulysin and Methotrexate for Folate Receptor Targeted Cancer Therapy
Authors: Truebenbach Ines , Gorges Jan , Kuhn Jasmin , Kern Sarah , Baratti Emanuele , Kazmaier Uli , Wagner Ernst , Lächelt Ulrich 
Published: March 2017

AbstractThe conjugation of small molecule drugs to ligand containing carrier systems facilitates receptor targeted delivery. The folate receptor (FR) constitutes an ideal target for tumor selective therapy, being overexpressed on several tumor types. It can be targeted using the vitamin folic acid (FolA) or the structurally related drug methotrexate (MTX). Several sequence‐defined oligoamides with mono‐ and multivalent FolA or MTX ligands and an additional thiol conjugation site are synthesized via solid‐phase assisted synthesis. Their structure activity relationships are assessed in respect to dihydrofolate reductase inhibition, receptor mediated endocytosis, and cytotoxicity. Then, the tubulin‐binding agent pretubulysin (PT), a highly potent drug exhibiting antitumoral, antiangiogenic, and antimetastatic properties, is conjugated via an activated mercaptane derivative to the set of FR‐targeting oligoamides. In a combined PT/MTX cytotoxicity study in FR‐overexpressing KB and L1210 cells, a 2‐arm MTX‐PT construct or the 4‐arm analog displays the highest potency in the respective cell lines.
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GABA editing with macromolecule suppression using an improved MEGA‐SPECIAL sequence
Authors: Gu Meng , Hurd Ralph , Noeske Ralph , Baltusis Laima , Hancock Roeland , Sacchet Matthew D. , Gotlib Ian H. , Chin Frederick T. , Spielman Daniel M. 
Published: March 2017

PurposeThe most common γ‐aminobutyric‐acid (GABA) editing approach, MEGA‐PRESS, uses J‐editing to measure GABA distinct from larger overlapping metabolites, but suffers contamination from coedited macromolecules (MMs) comprising 40 to 60% of the observed signal. MEGA‐SPECIAL is an alternative method with better MM suppression, but is not widely used primarily because of its relatively poor spatial localization. Our goal was to develop an improved MM‐suppressed GABA editing sequence at 3 Tesla.MethodsWe modified a single‐voxel MEGA‐SPECIAL sequence with an oscillating readout gradient for improved spatial localization, and used very selective 30‐ms editing pulses for improved suppression of coedited MMs.ResultsSimulation and in vivo experiments confirmed excellent MM suppression, insensitive to the range of B0 frequency drifts typically encountered in vivo. Both intersubject and intrasubject studies showed that MMs, when suppressed by the improved MEGA‐SPECIAL method, contributed approximately 40% to the corresponding MEGA‐PRESS measurements. From the intersubject study, the coefficient of variation for GABA+/Cre (MEGA‐PRESS) was 11.2% versus 7% for GABA/Cre (improved MEGA‐SPECIAL), demonstrating significantly reduced variance (P = 0.005), likely coming from coedited MMs.ConclusionsThis improved MEGA‐SPECIAL sequence provides unbiased GABA measurements with reduced variance as compared with conventional MEGA‐PRESS. This approach is also relatively insensitive to the range of B0 drifts typically observed in in vivo human studies. Magn Reson Med 79:41–47, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
Gastrointestinal toxicity among patients taking selective COX‐2 inhibitors or conventional NSAIDs, alone or combined with proton pump inhibitors: a case–control study
Authors: Bakhriansyah Mohammad , Souverein Patrick C. , de Boer Anthonius , Klungel Olaf H. 
Published: March 2017

AbstractPurposeTo assess the risk of gastrointestinal perforation, ulcers, or bleeding (PUB) associated with the use of conventional nonsteroidal anti‐inflammatory drugs (NSAIDs) with proton pump inhibitors (PPIs) and selective COX‐2 inhibitors, with or without PPIs compared with conventional NSAIDs.MethodsA case–control study was performed within conventional NSAIDs and/or selective COX‐2 inhibitors users identified from the Dutch PHARMO Record Linkage System in the period 1998–2012. Cases were patients aged ≥18 years with a first hospital admission for PUB. For each case, up to four controls were matched for age and sex at the date a case was hospitalized (index date). Logistic regression analysis was used to calculate odds ratios (ORs).ResultsAt the index date, 2634 cases and 5074 controls were current users of conventional NSAIDs or selective COX‐2 inhibitors. Compared with conventional NSAIDs, selective COX‐2 inhibitors with PPIs had the lowest risk of PUB (adjusted OR 0.51, 95% confidence interval [CI]: 0.35–0.73) followed by selective COX‐2 inhibitors (adjusted OR 0.66, 95%CI: 0.48–0.89) and conventional NSAIDs with PPIs (adjusted OR 0.79, 95%CI: 0.68–0.92). Compared with conventional NSAIDs, the risk of PUB was lower for those aged ≥75 years taking conventional NSAIDs with PPIs compared with younger patients (adjusted interaction OR 0.79, 95%CI: 0.64–0.99). However, those aged ≥75 years taking selective COX‐2 inhibitors, the risk was higher compared with younger patients (adjusted interaction OR 1.22, 95%CI: 1.01–1.47).ConclusionsSelective COX‐2 inhibitors with PPIs, selective COX‐2 inhibitors, and conventional NSAIDs with PPIs were associated with lower risks of PUB compared with conventional NSAIDs. These effects were modified by age. © 2017 The Authors. Pharmacoepidemiology & Drug Safety Published by John Wiley & Sons Ltd.
Invariant digital image watermarking based on Defragmented Delaunay Triangulation for optimal watermark synchronization
Authors: Essaidani Dhekra , Seddik Hassene , Ben Braiek Ezzedine 
Published: March 2017

AbstractDramatic technology progress in data manipulation induced several attempts of baleful and illegal processing. In this regard, several protection techniques, including cryptography, steganography, and watermarking have been used to avoid the illegal production and distribution of data online. On the other side, the attacks are still a major problem that limits the effectiveness of watermarking techniques. The geometric attacks involve displacement of pixels. Therefore, they induce synchronization errors between the original and attacked image that complicates the watermark recovery process. So in order to solve the de‐synchronization problem, we propose to use a robust meshing technique between different geometrical data of the image to correct the geometric distortion. In this work, we propose a novel geometrically invariant digital image watermarking approach based on the geometrical feature of the cover image and the Defragmented Delaunay Triangulation on respecting the three constraints of watermarking approaches (imperceptibility of embedded watermark, embedding capacity, and robustness). The aim idea of this work is to propose a blind, imperceptible, and robust digital image watermarking scheme based on Defragmented Delaunay Tessellation and Weber's law. The defragmented triangulation provides a best synchronization of the embedded data after attacks application. The Weber's law is used to propose an auto‐thresholding algorithm to compute the optimal embedding gain factor for each watermark's bit in order to ensure the imperceptibility of the hidden data. Firstly, the invariant features in the host gray scale image are extracted by using the canny edge detector. Then, the Delaunay tessellation of the saved keys points set is generated and defragmented to select the optimal robust triangles for watermark embedding. Simulation results illustrate the imperceptibility of the embedded data and the robustness of the proposed approach against intentional and unintentional geometrical attacks are presented in the finally section. Copyright © 2017 John Wiley & Sons, Ltd.
Cost‐effectiveness of diagnostic testing strategies including cervical‐length measurement and fibronectin testing in women with symptoms of preterm labor
Authors: van Baaren G.‐J. , Vis J. Y. , Wilms F. F. , Oudijk M. A. , Kwee A. , Porath M. M. , Scheepers H. C. J. , Spaanderman M. E. A. , Bloemenkamp K. W. M. , Haak M. C. , Bax C. J. , Cornette J. M. J. , Duvekot J. J. , Nij Bijvanck B. W. A. , van Eyck J. , Franssen M. T. M. , Sollie K. M. , Vandenbussche F. P. H. A. , Woiski M. , Bolte A. C. , van der Post J. A. M. , Bossuyt P. M. M. , Opmeer B. C. , Mol B. W. J. 
Published: March 2017

ABSTRACTObjectiveTo evaluate the cost‐effectiveness of combining cervical‐length (CL) measurement and fetal fibronectin (fFN) testing in women with symptoms of preterm labor between 24 and 34 weeks' gestation.MethodsThis was a model‐based cost‐effectiveness analysis evaluating seven test–treatment strategies based on CL measurement and/or fFN testing in women with symptoms of preterm labor from a societal perspective, in which neonatal outcomes and costs were weighted. Estimates of disease prevalence, test accuracy and costs were based on two recently performed nationwide cohort studies in The Netherlands.ResultsStrategies using fFN testing and CL measurement separately to predict preterm delivery are associated with higher costs and incidence of adverse neonatal outcomes compared with strategies that combine both tests. Additional fFN testing when CL is 15–30 mm was considered cost effective, leading to a cost saving of €3919 per woman when compared with a treat‐all strategy, with a small deterioration in neonatal health outcomes, namely one additional perinatal death and 21 adverse outcomes per 10 000 women with signs of preterm labor (incremental cost‐effectiveness ratios €39 million and €1.9 million, respectively). Implementing this strategy in The Netherlands, a country with about 180 000 deliveries annually, could lead to an annual cost saving of between €2.4 million and €7.6 million, with only a small deterioration in neonatal health outcomes.ConclusionIn women with symptoms of preterm labor at 24–34 weeks' gestation, performing additional fFN testing when CL is between 15 and 30 mm is a viable and cost‐saving strategy. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.