bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxic peptides with insulin‐releasing activities from skin secretions of the Italian stream frog Rana italica (Ranidae)
Authors: Conlon J. Michael , Musale Vishal , Attoub Samir , Mangoni Maria Luisa , Leprince Jérôme , Coquet Laurent , Jouenne Thierry , Abdel‐Wahab Yasser H. A. , Flatt Peter R. , Rinaldi Andrea C. 
Published: July 2017

Peptidomic analysis of norepinephrine‐stimulated skin secretions from Italian stream frog Rana italica led to the purification and characterization of two host‐defense peptides differing by a single amino acid residue belonging to the brevinin‐1 family (brevinin‐1ITa and ‐1ITb), a peptide belonging to the temporin family (temporin‐ITa) and a component identified as prokineticin Bv8. The secretions contained relatively high concentrations of the methionine‐sulphoxide forms of brevinin‐1ITa and ‐1ITb suggesting that these peptides may have a role as antioxidants in the skin of this montane frog. Brevinin‐1ITa (IVPFLLGMVPKLVCLITKKC) displayed potent cytotoxicity against non‐small cell lung adenocarcinoma A549 cells (LC50 = 18 μM), breast adenocarcinoma MDA‐MB‐231 cells (LC50 = 8 μM) and colorectal adenocarcinoma HT‐29 cells (LC50 = 18 μM), but the peptide was also strongly hemolytic against mouse erythrocytes (LC50 = 7 μM). Temporin‐ITa (VFLGAIAQALTSLLGKL.NH2) was between three and fivefold less potent against these cells. Brevinin‐1ITa inhibited growth of both Gram‐positive Staphylococcus epidermidis and Gram‐negative Escherichia coli as well as a strain of the opportunist yeast pathogen Candida parapsilosis, whereas temporin‐ITa was active only against S. epidermidis and C. parapsilosis. Both peptides stimulated the release of insulin from BRIN‐BD11 clonal β‐cells at concentrations ≥1 nM, but brevinin‐1ITa was cytotoxic to the cells at concentrations ≥3 μM. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
Isotopic homogeneity throughout the skin in small cetaceans
Authors: Arregui Marina , Josa Marta , Aguilar Alex , Borrell Asunción 
Published: July 2017

RationaleIsotope ratios from skin samples have been widely used to study cetacean trophic ecology. Usually, isotopic skin uniformity has been assumed, despite the heterogeneity of this tissue. This study aims to investigate (1) regional isotopic variation within the skin in cetaceans, and (2) isotopic variation among internal tissues.MethodsStable carbon (δ13C values) and nitrogen (δ15N values) isotope ratios were measured in 11 skin positions in 10 common dolphins (Delphinus delphis) and 9 striped dolphins (Stenella coeruleoalba). In addition, the isotope ratios in the muscle, liver and kidney of both species were determined and compared with those from the skin and from all tissues combined. The signatures were determined by means of elemental analyser/isotope ratio mass spectrometry (EA/IRMS).ResultsIn both species, no differences between isotope ratios of the skin positions were found. Moreover, the isotope ratios of skin were similar to those of muscle. In contrast, liver and kidney showed higher isotope ratios than muscle and skin.ConclusionsIsotopic homogeneity within the skin suggests that the isotope ratios of a sample from a specific skin position can be considered representative of the ratios from the entire skin tissue in dolphins. This conclusion validates the results of previous stable isotope analyses in dolphins that used skin samples as representative of the whole skin tissue. Isotopic similarities or dissimilarities among tissues should be considered when analysing different tissues and comparing results from the same or different species.
Dynamic modeling and nonlinear tracking control of a novel modified quadrotor
Authors: Tofigh Mohamad Ali , Mahjoob Mohamad J. , Ayati Moosa 
Published: July 2017

SummaryIn this article, a nonlinear tracking controller is designed based on Lyapunov stability for a novel aerial robot. The proposed 6‐rotor configuration improves stability and payload lifting capacity of the robot compared with conventional quadrotors while avoiding further complexities in the robot dynamics and steering principles. The dynamical model of the robot is derived using Newton‐Euler method. The model represents a nonlinear, coupled, and underactuated system. The proposed control strategy includes 2 main parts: an attitude controller and a position controller. Both the attitude and position controls are Lyapunov‐based nonlinear tracking controllers that guarantee the asymptotic convergence of the states' tracking errors to zero. Simulation results are presented to illustrate appropriate performance of the closed‐loop system in terms of position/attitude tracking even in the presence of wind disturbance.
Driving in search of analyses
Authors: Simons‐Morton Bruce 
Published: July 2017

AbstractAlthough transportation safety has greatly improved over the past 2 decades, motor vehicle crash injuries remain a leading cause of morbidity and mortality, particularly among young drivers. Driver errors and behaviors such as speeding and distraction contribute disproportionately to crashes among inexperienced novices, who develop safe driving judgment only with substantial driving experience, commonly described as the “young driver problem.” Research on young drivers has applied a range of research methods, including analyses of national archival data (mainly from police reports), crash analyses, observation of driver behavior, surveys of driver behavior and dispositions, and experimental research on driver behavior and vehicle crash worthiness. Prominent research questions regarding young driver safety include what and how do novices learn to drive safely, what are the predictors of young driver crashes, what is the variability and overtime trajectories of young driver performance and outcomes, and to what extent is the young driver problem due mainly to average population risk or high‐risk groups? Current research on young drivers is complicated by small sample sizes, relatively rare events, high within and between group variability, missing data, the need to estimate exposure, and the lack of longitudinal and experimental designs, problems that require complex analytic methods. In this paper, we provide an overview of driving research methods, examples of research addressing the young driver problem, and examples of statistical collaboration on young driver research, focusing particularly on estimating prediction of crash risk and estimating variability in young driver performance and outcomes.
Acetylated Signal Transducer and Activator of Transcription 3 Functions as Molecular Adaptor Independent of Transcriptional Activity During Human Cardiogenesis
Authors: Mehta Ashish , Ramachandra Chrishan J. A. , Chitre Anuja , Singh Pritpal , Lua Chong Hui , Shim Winston 
Published: July 2017

Abstract
Activation of signal transducer and activator of transcription 3 (STAT3) is imperative for mammalian development, specifically cardiogenesis. STAT3 phosphorylation and acetylation are key post-translational modifications that regulate its transcriptional activity. Significance of such modifications during human cardiogenesis remains elusive. Using human pluripotent stem cells to recapitulate cardiogenesis, two independently modified STAT3α (92 kDa) isoforms (phosphorylated and acetylated), which perform divergent functions were identified during cardiomyocyte (CM) formation. Phosphorylated STAT3α functioned as the canonical transcriptional activator, while acetylated STAT3α underwent caspase-3-mediated cleavage to generate a novel STAT3ζ fragment (∼45 kDa), which acted as a molecular adaptor integral to the ErbB4-p38γ signaling cascade in driving CM formation. While STAT3α knockdown perturbed cardiogenesis by eliminating both post-translationally modified STAT3α isoforms, caspase-3 knockdown specifically abrogates the function of acetylated STAT3α, resulting in limited STAT3ζ formation thereby preventing nuclear translocation of key cardiac transcription factor Nkx2-5 that disrupted CM formation. Our findings show the coexistence of two post-translationally modified STAT3α isoforms with distinct functions and define a new role for STAT3 as a molecular adaptor that functions independently of its canonical transcriptional activity during human cardiogenesis.
The energy dispersive scheme of X‐ray fluorescence analysis with a crystal polarizer and polycapillary optics
Authors: Turyanskiy A.G. , Gizha S.S. , Senkov V.M. , Stanishevskiy Ya.M. 
Published: July 2017

The efficiency of the polarization scheme based on polycapillary optics and a diamond crystal polarizer was demonstrated. The scheme provides suppression of the background of scattered radiation in measuring X‐ray fluorescence spectra. A quasi‐parallel X‐ray beam with an angular divergence of 4.2 mrad was formed by a microfocus source with a copper anode and polycapillary half‐lens. Simultaneous polarization and monochromatization of radiation was obtained with a crystal of natural diamond, which was set at the diffraction reflection (113). The degree of polarization of CuKα1 spectral line and the maximum radiation flux were respectively equal to 99.86% and 5 · 106 photon/s. In the direction orthogonal to the plane of diffraction, the maximum attenuation of the background was up to 19 dB.
Carbohydrase enzymes improve performance of broilers fed both nutritionally adequate and marginal wheat-based diets
Authors: Wu D. , Choct M. , Wu S. B. , Liu Y. G. , Swick R. A. 
Published: July 2017

SummaryA study was conducted to examine the effects of a multi-carbohydrase enzyme complex on the nutritive value of wheat in diets differing in nutrient density. It was hypothesised that response to enzyme inclusion would be greater in diets with lower nutrient density. The study was conducted using 1008 Ross 308 male broiler chicks (four treatments with seven replicate pens of 36 chicks). A 2 × 2 factorial arrangement of treatments was employed. Factors were adequate or low nutrient density with or without enzyme supplementation. The wheat-soybean meal based positive control (PC) diet was formulated to be nutritionally adequate in energy and digestible amino acids according to local industry recommendations. A negative control (NC) was formulated to have 80 kcal/kg less ME and 1.5% less digestible amino acids as compared to the PC. A multi-carbohydrase complex containing 19 carbohydrase activities derived from Penicillium funiculosum was added in both the PC and NC diets (Rovabio® Excel LC, Adisseo Asia Pacific Pte Ltd., Singapore). Birds fed the NC had 3.7 points (P < 0.05) poorer FCR than the PC. Across the diet type, enzyme supplementation increased body weight by 3.2% (P < 0.05) and improved FCR by 5.2 points (P < 0.01). There was no nutrient density x enzyme interaction (P > 0.05), indicating that performance improvement was independent of nutrient density. Apparent ileal digestibility of crude protein followed a similar trend, showing a 4.9% enhancement (P < 0.01) with the inclusion of the enzyme product in either diet. Enzyme supplementation reduced ileal viscosity by 39.0% (P < 0.05). It was concluded that multi-carbohydrase could overcome the negative effect in broiler performance brought by nutrient reduction, however, there was no indication that nutrient density affected bird response to supplementation of multi-carbohydrase.
Oriental Metrology and the Politics of Antiquity in Nineteenth-Century Survey Sciences
Authors: Schaffer Simon 
Published: July 2017

ArgumentMetrological techniques to establish shared quantitative measures have often been seen as signs of rational modernization. The cases considered here show instead the close relation of such techniques with antiquarian and revivalist programs under imperial regimes. Enterprises in survey sciences in Egypt in the wake of the French invasion of 1798 and in India during the East India Company's revenue surveys involved the promotion of a new kind oforiental metrologydesigned to represent colonizers’ measures as restorations of ancient values to be applied to current systems of survey and measurement. Surveyors’ practice and hardware help clarify the significance of the complex historical and political functions of scientific standards. The balance of the paper discusses the survey work of later nineteenth-century indigenous Egyptian astronomers at a conjuncture of major economic and political dislocation to explore the various versions of antiquity at stake in these metrological programs.
Measuring Age-Related Differences in Using a Simple Decision Strategy
Authors: Pohl Rüdiger F. 
Published: July 2017

Abstract. According to the recognition heuristic, decision makers base their inferences on recognition alone, assuming that recognized objects have larger criterion values than unrecognized ones. Knowing that recognition is a valid cue and thus using the recognition heuristic should increase with age. This was tested in two experiments with preadolescents (N = 140), adolescents (N = 186), and adults (N = 78). The results show, as expected, a monotonic age-related trend in the improvement of domain-specific knowledge but, unexpectedly, a non-monotonic one for using the recognition heuristic. More specifically, use of the recognition heuristic increased from preadolescents to adolescents, but then dropped for adults.
LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation
Authors: Raab Monika , Lu Yuning , Kohler Karsten , Smith Xin , Strebhardt Klaus , Rudd Christopher E. 
Published: July 2017

AbstractLymphocyte function-associated antigen 1 (LFA-1) affinity and avidity changes have been assumed to mediate adhesion to intercellular adhesion molecule-1 for T-cell conjugation to dendritic cells (DC). Although the T-cell receptor (TCR) and LFA-1 can generate intracellular signals, the immune cell adaptor protein linker for the activation of T cells (LAT) couples the TCR to downstream events. Here, we show that LFA-1 can mediate both adhesion and de-adhesion, dependent on receptor clustering. Although increased affinity mediates adhesion, LFA-1 cross-linking induced the association and activation of the protein-tyrosine kinases FAK1/PYK1 that phosphorylated LAT selectively on a single Y-171 site for the binding to adaptor complex GRB-2-SKAP1. LAT-GRB2-SKAP1 complexes were distinct from canonical LAT-GADs-SLP-76 complexes. LFA-1 cross-linking increased the presence of LAT-GRB2-SKAP1 complexes relative to LAT-GADs-SLP-76 complexes. LFA-1-FAK1 decreased T-cell-dendritic cell (DC) dwell times dependent on LAT-Y171, leading to reduced DO11.10 T cell binding to DCs and proliferation to OVA peptide. Overall, our findings outline a new model for LFA-1 in which the integrin can mediate both adhesion and de-adhesion events dependent on receptor cross-linking.
Loss of mTORC1 signalling impairs β-cell homeostasis and insulin processing
Authors: Blandino-Rosano Manuel , Barbaresso Rebecca , Jimenez-Palomares Margarita , Bozadjieva Nadejda , Werneck-de-Castro Joao Pedro , Hatanaka Masayuki , Mirmira Raghavendra G. , Sonenberg Nahum , Liu Ming , Rüegg Markus A. , Hall Michael N. , Bernal-Mizrachi Ernesto 
Published: July 2017

AbstractDeregulation of mTOR complex 1 (mTORC1) signalling increases the risk for metabolic diseases, including type 2 diabetes. Here we show that β-cell-specific loss of mTORC1 causes diabetes and β-cell failure due to defects in proliferation, autophagy, apoptosis and insulin secretion by using mice with conditional (βraKO) and inducible (MIP-βraKOf/f) raptor deletion. Through genetic reconstitution of mTORC1 downstream targets, we identify mTORC1/S6K pathway as the mechanism by which mTORC1 regulates β-cell apoptosis, size and autophagy, whereas mTORC1/4E-BP2-eIF4E pathway regulates β-cell proliferation. Restoration of both pathways partially recovers β-cell mass and hyperglycaemia. This study also demonstrates a central role of mTORC1 in controlling insulin processing by regulating cap-dependent translation of carboxypeptidase E in a 4EBP2/eIF4E-dependent manner. Rapamycin treatment decreases CPE expression and insulin secretion in mice and human islets. We suggest an important role of mTORC1 in β-cells and identify downstream pathways driving β-cell mass, function and insulin processing.
A novel ADOA-associated OPA1 mutation alters the mitochondrial function, membrane potential, ROS production and apoptosis
Authors: Zhang Juanjuan , Liu Xiaoling , Liang Xiaoyang , Lu Yuanyuan , Zhu Ling , Fu Runing , Ji Yanchun , Fan Wenlu , Chen Jie , Lin Bing , Yuan Yimin , Jiang Pingping , Zhou Xiangtian , Guan Min-Xin 
Published: July 2017

AbstractAutosomal dominant optic atrophy (ADOA) is a dominantly inherited optic neuropathy, affecting the specific loss of retinal ganglion cells (RGCs). The majority of affected cases of ADOA are associated with mutations in OPA1 gene. Our previous investigation identified the c.1198C > G (p.P400A) mutation in the OPA1 in a large Han Chinese family with ADOA. In this report, we performed a functional characterization using lymphoblostoid cell lines derived from affected members of this family and control subjects. Mutant cell lines exhibited the aberrant mitochondrial morphology. A ~24.6% decrease in the mitochondrial DNA (mtDNA) copy number was observed in mutant cell lines, as compared with controls. Western blotting analysis revealed the variable reductions (~45.7%) in four mtDNA-encoded polypeptides in mutant cell lines. The impaired mitochondrial translation caused defects in respiratory capacity. Furthermore, defects in mitochondrial ATP synthesis and mitochondrial membrane potential (ΔΨm) were observed in mutant cell lines. These abnormalities resulted in the accumulation of oxidative damage and increasing of apoptosis in the mutant cell lines, as compared with controls. All those alterations may cause the primary degeneration of RGCs and subsequent visual loss. These data provided the direct evidence for c.1198C > G mutation leading to ADOA. Our findings may provide new insights into the understanding of pathophysiology of ADOA.
Nanoplasmonic fiber tip probe detects significant reduction of intracellular Alzheimer’s disease-related oligomers by curcumin
Authors: Liang Feng , Wan Yu , Schaak Diane , Ward Joseph , Shen Xunuo , Tanzi Rudolph E. , Zhang Can , Quan Qimin 
Published: July 2017

AbstractConsiderable evidence shows critical roles of intracellular pathogenic events of Alzheimer’s disease (AD). In particular, intracellular amyloid-β accumulation and oligomerization are early AD pathologic processes, which may lead to changes in inflammatory molecules and other AD-related pathological components. Curcumin and its analogs have been identified as potential drug candidates for AD. However, the effects of curcumin on intracellular AD pathologic processes remain largely unknown. Here we utilized a recently developed nanoplasmonic fiber tip probe (nFTP) technology and investigated whether curcumin leads to intracellular AD pathologic changes. We showed that our nFTP technology could robustly detect intracellular AD-related protein changes caused by a well-known inflammation inducer and a familial AD mutation. Intriguingly, curcumin remarkably reduced the level of intracellular oligomers while modestly reduced the level of an inflammatory cytokine. Thus, our results provided evidence that curcumin’s mechanism of action in attenuating AD pathology is through a major role of decreasing oligomerization.
Iron overload exacerbates age-associated cardiac hypertrophy in a mouse model of hemochromatosis
Authors: Sukumaran Abitha , Chang JuOae , Han Murui , Mintri Shrutika , Khaw Ban-An , Kim Jonghan 
Published: July 2017

AbstractCardiac damage associated with iron overload is the most common cause of morbidity and mortality in patients with hereditary hemochromatosis, but the precise mechanisms leading to disease progression are largely unexplored. Here we investigated the effects of iron overload and age on cardiac hypertrophy using 1-, 5- and 12-month old Hfe-deficient mice, an animal model of hemochromatosis in humans. Cardiac iron levels increased progressively with age, which was exacerbated in Hfe-deficient mice. The heart/body weight ratios were greater in Hfe-deficient mice at 5- and 12-month old, compared with their age-matched wild-type controls. Cardiac hypertrophy in 12-month old Hfe-deficient mice was consistent with decreased alpha myosin and increased beta myosin heavy chains, suggesting an alpha-to-beta conversion with age. This was accompanied by cardiac fibrosis and up-regulation of NFAT-c2, reflecting increased calcineurin/NFAT signaling in myocyte hypertrophy. Moreover, there was an age-dependent increase in the cardiac isoprostane levels in Hfe-deficient mice, indicating elevated oxidative stress. Also, rats fed high-iron diet demonstrated increased heart-to-body weight ratios, alpha myosin heavy chain and cardiac isoprostane levels, suggesting that iron overload promotes oxidative stress and cardiac hypertrophy. Our findings provide a molecular basis for the progression of age-dependent cardiac stress exacerbated by iron overload hemochromatosis.
Hypertonic saline infusion suppresses apoptosis of hippocampal cells in a rat model of cardiopulmonary resuscitation
Authors: Zhou Xiang , Liu Yong , Huang Yang , Zhu ShuiBo , Zhu Jian , Wang RongPing 
Published: July 2017

AbstractHypertonic saline (HS) attenuates cerebral edema, improves microcirculation perfusion and alleviates inflammation. However, whether the beneficial effect of HS on neurological function after cardiopulmonary resuscitation (CPR) in rat model of asphyxial cardiac arrest (CA) is mediated via attenuating apoptosis of neurons is not known. We studied the neuroprotective effect of HS in rats after CA and CPR, and explored the likely underlying mechanisms. Animals were randomly assigned to 4 equal groups (n = 15 each) according to the different infusions administered during resuscitation: control (C), normal saline (NS), hypertonic saline (HS), and hydroxyethyl starch (HES) groups. NDS at 12, 24, 48 and 72 h post-ROSC in the HS group were significantly higher than those in the NS and HES groups. Western blot analysis demonstrated a significant increase in Bcl-2 expression in HS, as compared to that in the NS and HES groups. However, Bax and Caspase-3 expressions in HS were significantly lower than that in the NS and HES groups. The apoptosis rate in HS was significantly lower than that in the NS and HES groups, suggesting HS treatment during resuscitation could effectively suppress neuronal cell apoptosis in hippocampal CA1 post-ROSC and improve neuronal function.
Spatio-temporal variations of typhoid and paratyphoid fevers in Zhejiang Province, China from 2005 to 2015
Authors: Gu Hua , Fan Wenjie , Liu Kui , Qin Shuwen , Li Xiuyang , Jiang Jianmin , Chen Enfu , Zhou Yibiao , Jiang Qingwu 
Published: July 2017

AbstractTyphoid and paratyphoid are two common enteric infectious diseases with serious gastrointestinal symptoms. Data was collected of the registered cases in Zhejiang Province from 2005 to 2015. The epidemiological characteristics were investigated and high-risk regions were detected with descriptive epidemiological methods and in-depth spatio-temporal statistics. A sharp decline in the incidences of both diseases was observed. The seasonal patterns were identified with typhoid and paratyphoid, one in summer from May to September was observed from 2005 to 2010 and the other lesser one in spring from January to March only observed from 2005 to 2007. The men were more susceptible and the adults aged 20 to 60 constituted the major infected population. The farmers were more likely to get infected, especially to typhoid. The Wilcoxon sum rank test proved that the incidences in the coastal counties were significantly higher than the inland. Besides, a positive autocorrelation was obtained with typhoid fever in global autocorrelation analysis but not with paratyphoid fever. Local autocorrelation analysis and spatio-temporal scan statistics revealed that high-risk clusters were located mainly in the coastal regions with typhoid fever but scattered across the province with paratyphoid fever. The spatial risks were evaluated quantitatively with hierarchical Bayesian models.