bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality and nutritional attributes of whole einkorn flour and its suitability for fresh noodle production compared to whole wheat flour
Authors: Kim Jeongeon , Moon Yujin , Kim Hyungseop , Gőksen Gűlden , Kweon Meera 
Published: September 2024

AbstractBackground and ObjectivesThis study investigated the physicochemical properties, particle size, pasting properties, phenolic acids, carotenoid content, and fresh noodle quality characteristics of whole einkorn flour (WEF) in comparison with whole wheat flour (WWF). Three WEF samples from different cultivation regions in Turkey were analyzed.FindingsThe WEFs exhibited significant variations in all quality characteristics compared to WWF, influenced by different einkorn growing environments. Phenolic acid and carotenoid contents, along with antioxidant activity, were highest in WEF1 and WEF2, highlighting their potential nutritional benefits. Noodles made from WEF3 demonstrated quality equivalent to that of WWF, although their protein content and nutritional value were relatively lower than those of WEF1 and WEF2.ConclusionsThis research demonstrates the potential of WEF for fresh noodle production with quality comparable to WWF. The nutritional value varies by cultivation area, emphasizing the importance of selecting appropriate cultivars and growing conditions for optimal results.Significance and NoveltyThis study highlights, for the first time, the nutritional value and quality characteristics of WEF influenced by growing areas, impacting its suitability, equivalent to WWF, for producing fresh noodles.
Lactic acid bacteria‐fermented Chlamydomonas reinhardtii as an innovative ingredient to produce microalgae‐enriched steamed bread
Authors: Jin Zhiqiang , Gu Yaoguang , Zhang Wen 
Published: September 2024

AbstractBackground and ObjectivesChlamydomonas reinhardtii (C. reinhardtii) is recognized as a sustainable source of high‐quality protein. This study aimed to assess the potential application of C. reinhardtii for producing microalgae‐fortified steamed bread. To reduce the inherent green color and volatile compounds of the microalgae, lactic acid bacteria (LAB) fermentation was applied as a pretreatment method for C. reinhardtii. Subsequently, both unfermented (unFC) and fermented C. reinhardtii (FC) were added to steamed bread formulations (4%, 8%, 12%, and 16% wheat flour substitution).FindingsThe results showed that the incorporation of unFC into steamed bread had a dose‐dependent negative impact on the dough fermentation performance and the quality of steamed bread, including volume, texture, and sensory attributes, rendering the steamed bread unacceptable. Fermentation altered the amino acid profile of C. reinhardtii, caused the color to shift from green to red (a*, −14.7 vs. 7.0), and reduced unpleasant volatile components. Further, fermentation reduced the negative impact of microalgae incorporation, especially at substitution levels of 4% and 8%, which manifested as an increased dough fermentation volume and improved bread volume with reduced crumb firmness of the steamed bread. However, in sensory analysis, the steamed bread that contained 4% FC had an overall acceptability similar to the control sample.ConclusionsMicroalgae‐enriched steamed bread could be produced by incorporating up to 4% FC to improve its nutritional profiles while maintaining overall sensory acceptability by the consumers.Significance and NoveltyLAB fermentation of C. reinhardtii is a feasible strategy to address the sensory and structural challenges that hinder the incorporation of algae into food products.
Pd Nanoparticle Supported on N‐Doped Carbon Derived from Sodium Alginate/Melamine Blends as Efficient Heterogeneous Catalyst for Heck Reactions
Authors: Zhou Jie , Wu Yuanyuan , Liu Yonghong , Li Na , Yang Zhen , Ying Jiadi , Ren Xiaorong , Zhang Tao , Xu Wei , Chen Jinyang , Cao Xingzhong , Yu Runsheng , Zeng Minfeng 
Published: September 2024

AbstractN‐doped porous carbon shows great potential in the field of heterogenous supports due to its high porosity, large surface area, rich active sites, and strong chelation with catalytic active metals species. Herein, we successfully prepared a novel N‐doped porous carbon derived from sodium alginate/melamine blends (SAMNC) with different mass ratio through a simple carbonization and activation process. Hierarchical porous structure of the derived N‐doped carbon has been confirmed with the SEM, TEM, and N2 adsorption characterization. After Na2PdCl4 solution impregnation and further reduction process, Pd0 nanoparticles have been uniformly immobilized on the SAMNC support to produce novel Pd@SAMNC catalyst. The optimal Pd@SAMNC‐0.6 possesses a high N content (5.13%), Pd content (5.90%), large BET specific surface area of 1884.5 m2·g−1. Positron annihilation lifetime spectroscopy (PALS) investigation of the porous carbon materials provided the sub‐nano level microporous information proofs of Pd@SAMNC‐0.6 had the capability to provide more active sites for reactions than commercial Pd supported on activated carbon (Pd@AC). Pd@SAMNC‐0.6 catalyst showed superior catalytic efficiency in Heck coupling reaction between aromatic halides and alkenes and can be recycled for 17 runs without significant decrease in catalytic efficiency.
Leveraging Natural Product‐Inspired Antifungals to Investigate the Mechanism of Action of Peniciaculin A
Authors: Wilt Ingrid K. , Demeritte Adrian R. , Kim Alexander T. , Wang Weiwei , Wuest William M. 
Published: September 2024

AbstractUbiquinone mimics known as quinone outside inhibitors (QoIs) are one of the most prominent fungicides used to protect crops in the agricultural industry. Due to chemotype similarities with known QoIs, peniciaculin A, a triaryl natural product, was proposed to exhibit similar broad spectrum antifungal activity against phytopathogens. Instability of the tertiary alcohol and phenol motif, however, prompted exploration of the antifungal properties of simplified analogues to probe possible overlap in mechanism of action between the natural product and QoIs. Peniciaculin A inspired analogues mimicking known QoI scaffolds displayed broad spectrum antifungal activity while those containing scaffolds dissimilar to QoIs possessed negligible bioactivity. These activity profiles suggest peniciaculin A is likely acting as a QoI.
Programmed death‐ligand 1 expression in patients with primary or secondary myelofibrosis
Authors: Ibrahim Moayed , Murphree Catherine , Patel Kirtesh , Mastrodomenico Matthew , Saba Nakhle S. , Safah Hana , Schmid Janet , Socola Francisco 
Published: September 2024

AbstractBackgroundIt has been described in mice models that myeloproliferative neoplasm (MPN) with JAK2‐V617F mutation has an increased expression of programmed death‐ligand 1 (PD‐L1) in megakaryocytes leading to cancer immune evasion by inhibiting the T‐lymphocytes.AimsTo quantify and compare the PD‐L1 expression on bone marrow (BM) of patients with MPN JAK2 positive, negative, and normal controls.MethodsWe collected BM of patients with MPN JAK2 positive, negative and normal controls from 1990 to 2019. We also created a scoring system to quantify PD‐L1 expression in megakaryocytes.ResultsWe obtained 14 BM with JAK2 positive PMF, 5 JAK2 negative PMF, and 10 patients with normal BM biopsies. PD‐L1 expression was higher in the JAK2 positive group compared with the control group with a score of 212.6 versus 121.1 (t‐value 2.05, p‐value 0.025). In addition, the score was higher in the PMF group regardless of JAK2 mutational status when compared with the control group with score of 205.9 versus 121.1 (t‐value 2.12, p‐value 0.021). There was no difference in the PD‐L1 score between the JAK2 negative versus the control group 187.2 versus 121.1 (t‐value 1.02, p‐value 0.162).ConclusionThese findings suggest that PMF patients with a JAK2 mutation have a higher PD‐L1 expression in megakaryocytes compared with the control group. We postulate that the combination of checkpoint and JAK2 inhibitors may be an active treatment option in JAK2 mutated PMF given the higher PD‐L1 expression.
Experiences Using Nonpharmacological Interventions for Chronic Fatigue: A Focus Group Study of Long‐Term Survivors of Young Adult Cancers With Fatigue
Authors: Stub Trine , Mathisen Marleen , Thorsen Lene , Kiserud Cecilie E. , Lie Hanne C. 
Published: September 2024

ABSTRACTBackgroundCancer‐related fatigue is a common and distressing late effect of cancer that can persist for decades after treatment completion. Although negatively affecting survivors' quality of life, few, if any, efficacious interventions for persistent, or chronic, fatigue exist.AimsTo inform future interventions, we explored how long‐term, young adult cancer survivors (YACSs) with chronic fatigue live with, and manage their fatigue over time, including their experiences with nonpharmacological interventions (NPIs) for chronic fatigue.Methods and ResultsWe conducted a qualitative focus group study with 15 YACSs (13 women) with chronic fatigue, on average 7.3 years post‐diagnosis. The YACS were identified and recruited through a nationwide health survey of cancer survivors (the NOR‐CAYACS study). Systematic content analysis was used to identify recurrent themes. Analysis revealed five themes: (1) manifestation of fatigue, detailing chronic fatigue experiences; (2) impact on daily life, highlighting the necessity to balance rest and activity, affecting relationships; (3) NPIs, where walks in nature were notably beneficial; (4) barriers to fatigue management, including energy deficits, treatment‐related bodily changes, and self‐care prioritization challenges; (5) facilitators to fatigue management, emphasizing the need for regular breaks, self‐care practices, and the importance of fatigue management education.ConclusionThis study offers novel insights into the lived experiences of YACSs with chronic fatigue, a subject scarcely examined in prior research. Our findings highlight the significant impact of chronic fatigue and the individualized strategies YACSs use to cope. The research emphasizes the need for personalized interventions to support chronic fatigue management, marking a critical step forward in addressing this often‐overlooked issue in survivorship care. Future research should focus on tailored approaches to improve YACSs' quality of life.
The Unified Protocol or Dialectical Behaviour Therapy? Considerations for Choosing Between Two Evidence‐Based Transdiagnostic Psychotherapies for Complex Patients
Authors: Cassiello‐Robbins Clair , Ritschel Lorie A. , Mochrie Kirk D. , Edwards‐Powell Becca , Byars Kathryn 
Published: September 2024

ABSTRACTThe advent of multiple transdiagnostic treatments in recent decades has advanced the field of clinical psychology while also raising questions for clinicians and patients about how to decide between treatments and how to best deliver a chosen treatment. The purpose of this paper is to review two prominent transdiagnostic treatments that target emotion dysregulation: dialectical behaviour therapy and the unified protocol for transdiagnostic treatment for emotional disorders. First, we review the theoretical underpinnings, research support and proposed mechanisms of action for these treatments. Next, we discuss patient and therapist variables that might indicate which treatment is more appropriate for a given patient and discuss decision‐making guidelines to help make this determination with an emphasis on complex patients who may present with risk and/or clinical comorbidities. Finally, we discuss areas for future research that can help further ensure we work to match patients to the treatment that is most likely to benefit them.
A comprehensive study on in situ stress field characteristics and changes in rock mechanical properties in deep mines in northeastern Yunnan, China
Authors: Wang Hui , Sun Bangtao , Cao Cong , Yu Shibo , Wang He , Yuan Ye , Zhong Hua 
Published: September 2024

AbstractThe Maoping lead–zinc mining area is a significant metal mine site in northeastern Yunnan. In this study, both hydraulic fracturing in situ stress testing and ultrasonic imaging logging were first carried out in the mining area. Second, 930 focal mechanism solutions and 231 sets of stress data near the mining area were collected. Then, the variations in the type of in situ stress field, the magnitude of in situ stress, the direction of horizontal principal stress, and the ratio of lateral pressure were analyzed to characterize the distribution of the in situ stress field. On this basis, a new method using borehole breakouts and drilling‐induced fractures was proposed to determine the stress direction. Finally, the evolution of the mechanical properties of dolomite with burial depth was analyzed and the influence of rock mechanical properties on the distributions of the in situ stress field was explored. The results show that the in situ stress in the mining area is σH > σV > σh, indicating a strike–slip stress state. The in situ stress is high in magnitude, and its value increases with burial depth. The maximum and minimum horizontal lateral stress coefficients are stabilized at approximately 1.22 and 0.73, respectively. The direction of the maximum horizontal principal stress is NW, mainly ranging from N58.44° W to N59.70° W. The stress field inferred from the focal mechanism solution is in good agreement with the test results. The proportion of structural planes with dip angles between 30° and 75° exceeds 80%, and the dip direction of the structural planes is mainly NW to NWW. The line density of structural planes shows high density in shallow areas and low density in deep areas. More energy tends to be accumulated in rocks with higher elastic modulus and strength, leading to higher in situ stress levels. These findings are of significant reference for mine tunnel layout, support design optimization, and disaster prevention.
Climatic niche shifts and ecological sky‐island dynamics in Mesoamerican montane birds
Authors: Rocha‐Méndez Alberto , Prieto‐Torres David A. , Sánchez‐González Luis A. , Navarro‐Sigüenza Adolfo G. 
Published: September 2024

AbstractAn ongoing challenge in evolutionary and ecological research focuses on testing biogeographic hypotheses for the understanding of both species' distributional patterns and of the factors influencing range limits. In this study, we described the climatic niches of Neotropical humid montane forest birds through the analysis of factors driving their evolution at inter‐ and intraspecific levels; and tested for differences among allopatric lineages within Aulacorhynchus, Chlorospingus, Cardellina, and Eupherusa. We employed ecological niche models (ENMs) along with an ordination approach with kernel smoothing to perform niche overlap analyses and test hypotheses of niche equivalence/similarity among lineages. In addition, we described the potential distributions of each lineage during the Late Pleistocene climate fluctuations, identifying historical range expansions, connectivity, and stability. Overall, we observed differences in environmental variables influencing climatic requirements and distributional patterns for our selected species. We detected the highest values of niche overlap mainly between Eupherusa and some Chlorospingus lineages. At both interspecific and intraspecific levels, sister lineages showed non‐identical environmental niches. Our results offer weak support to a moist forest model, in which populations followed the expansion and contraction cycles of montane forests, leading to a lack of niche conservatism among lineages (they tend to occupy not identical climatic environments) throughout Mesoamerica. Therefore, historical climatic conditions may act as ecological barriers determining the distributional ranges of these species.
Macroinvertebrates but Not Diatoms Are Affected by Streamflow Alteration Below Hydropower Diversions
Authors: Spitale Daniel , Fezzi Marco , Zorzi Nadia , Slomp Elisabeth , Rigotti Sandro , Borrini Alex , Bilous Olena , Cantonati Marco 
Published: September 2024

ABSTRACTRiver regulation due to dams and other intake structures has impacted the hydrology, water quality and biology of rivers worldwide. The release of minimum flows still represents the strategy aimed at maintaining certain aspects of the original flow patterns. However, there remains a limited understanding of the consequences of artificially induced flows on water quality and aquatic life across various types of rivers. This study was conducted in Trentino (south‐eastern Alps, Italy) in 60 perennial river reaches (400–2005 m a.s.l.), all located downstream of water abstractions for different hydropower plants. The main goal of this research was to compare the effect of different residual flows on macroinvertebrates and diatoms. The overall effect of discharge was low but still significant only for macroinvertebrates, whereas diatoms were affected mostly by the water chemistry. Both groups responded to the substrate composition and to the hydromorphology, which in turn resulted to be strongly correlated. The biotic indices, originally developed to respond to pollution, were unaffected by the flow reduction, raising concerns about the potential consequences that assessment based on this approach may have on the evaluation of ecological flow. This study emphasizes the importance of considering the broader ecological context in which flow alterations occur, particularly the interaction of hydromorphology and substrate, in understanding their impact on aquatic biota.
Testing food web theory in a large lake: The role of body size in habitat coupling in Lake Michigan
Authors: Maitland Bryan M. , Bootsma Harvey A. , Bronte Charles R. , Bunnell David B. , Feiner Zachary S. , Fenske Kari H. , Fetzer William W. , Foley Carolyn J. , Gerig Brandon S. , Happel Austin , Höök Tomas O. , Keppeler Friedrich W. , Kornis Matthew S. , Lepak Ryan F. , McNaught A. Scott , Roth Brian M. , Turschak Benjamin A. , Hoffman Joel C. , Jensen Olaf P. 
Published: September 2024

AbstractThe landscape theory of food web architecture (LTFWA) describes relationships among body size, trophic position, mobility, and energy channels that serve to couple heterogenous habitats, which in turn promotes long‐term system stability. However, empirical tests of the LTFWA are rare and support differs among terrestrial, freshwater, and marine systems. Further, it is unclear whether the theory applies in highly altered ecosystems dominated by introduced species such as the Laurentian Great Lakes. Here, we provide an empirical test of the LTFWA by relating body size, trophic position, and the coupling of different energy channels using stable isotope data from species throughout the Lake Michigan food web. We found that body size was positively related to trophic position, but for a given trophic position, organisms predominately supported by pelagic energy had smaller body sizes than organisms predominately supported by nearshore benthic energy. We also found a hump‐shaped trophic relationship in the food web where there is a gradual increase in the coupling of pelagic and nearshore energy channels with larger body sizes as well as higher trophic positions. This highlights the important role of body size and connectivity among habitats in structuring food webs. However, important deviations from expectations are suggestive of how species introductions and other anthropogenic impacts can affect food web structure in large lakes. First, native top predators appear to be flexible couplers that may provide food web resilience, whereas introduced top predators may confer less stability when they specialize on a single energy pathway. Second, some smaller bodied prey fish and invertebrates, in addition to mobile predators, coupled energy from pelagic and nearshore energy channels, which suggests that some prey species may also be important integrators of energy pathways in the system. We conclude that patterns predicted by the LTFWA are present in the face of species introductions and other anthropogenic stressors to a degree, but time‐series evaluations are needed to fully understand the mechanisms that promote stability.
Integrin beta‐like 1 is regulated by DNA methylation and increased in heart failure patients
Authors: Kerrigan Lauren , Edgar Kevin , Russell‐Hallinan Adam , Cappa Oisin , Glezeva Nadezhda , Galan‐Arriola Carlos , Oliver Eduardo , Ibanez Borja , Baugh John , Collier Patrick , Ledwidge Mark , McDonald Ken , Simpson David , Das Sudipto , Grieve David J. , Watson Chris J. 
Published: September 2024

AbstractAimsDynamic alterations in cardiac DNA methylation have been implicated in the development of heart failure (HF) with evidence of ischaemic heart disease (IHD); however, there is limited research into cell specific, DNA methylation sensitive genes that are affected by dysregulated DNA methylation patterns. In this study, we aimed to identify DNA methylation sensitive genes in the ischaemic heart and elucidate their role in cardiac fibrosis.MethodsA multi‐omics integrative analysis was carried out on RNA sequencing and methylation sequencing on HF with IHD (n = 9) versus non‐failing (n = 9) left ventricular tissue, which identified Integrin beta‐like 1 (ITGBL1) as a gene of interest. Expression of Itgbl1 was assessed in three animal models of HF; an ischaemia‐reperfusion pig model, a myocardial infarction mouse model and an angiotensin‐II infused mouse model. Single nuclei RNA sequencing was carried out on heart tissue from angiotensin‐II infused mice to establish the expression profile of Itgbl1 across cardiac cell populations. Subsequent in vitro analyses were conducted to elucidate a role for ITGBL1 in human cardiac fibroblasts. DNA pyrosequencing was applied to assess ITGBL1 CpG methylation status in genomic DNA from human cardiac tissue and stimulated cardiac fibroblasts.ResultsITGBL1 was >2‐fold up‐regulated (FDR adj P = 0.03) and >10‐fold hypomethylated (FDR adj P = 0.01) in human HF with IHD left ventricular tissue compared with non‐failing controls. Expression of Itgbl1 was up‐regulated in three isolated animal models of HF and showed conserved correlation between increased Itgbl1 and diastolic dysfunction. Single nuclei RNA sequencing highlighted that Itgbl1 is primarily expressed in cardiac fibroblasts, while functional studies elucidated a role for ITGBL1 in cardiac fibroblast migration, evident in 50% reduced 24 h fibroblast wound closure occurring subsequent to siRNA‐targeted ITGBL1 knockdown. Lastly, evidence provided from DNA pyrosequencing supports the theory that differential expression of ITGBL1 is caused by DNA hypomethylation.ConclusionsITGBL1 is a gene that is mainly expressed in fibroblasts, plays an important role in cardiac fibroblast migration, and whose expression is significantly increased in the failing heart. The mechanism by which increased ITGBL1 occurs is through DNA hypomethylation.