bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toward improved human health: Ferroptosis‐related “Hyperfoods” as the nutritional supplement in COVID‐19
Authors: Zhao Yanan , Cao Hui , Ma Linyin , Wang Zixu , Guan Shuang , Teng Hui , Chen Lei 
Published: September 2024

AbstractThe outbreak of coronavirus disease 2019 (COVID‐19) stimulates a body of studies for better investigating the essential mechanisms and clinical characteristics of this disease. Notwithstanding, the underlying mechanisms of multiple organ failure (MOF) remain unclear. Understanding the mechanism of COVID‐19‐stimulated programmed cell death (RCD) may contribute to better strategies for these patients. Based on the exploration of COVID‐19‐related basic science reports and clinical research, we speculate that ferroptosis, a recently identified RCD, might be a substantial stimulator for MOF in COVID‐19, and it may act as a novel treatment target. However, the published investigations to determine how ferroptosis works in severe acute respiratory syndrome coronavirus 2 infection are still lacking. This review reveals new crucial missing links in our prevailing understanding of the mechanisms that ferroptosis‐triggered MOF in COVID‐19 patients. We also specifically summarize several literatures evidence about the functional food components as the nutritional supplement for COVID‐19 by its characteristics of ferroptosis inhibition. We hope this hypothesis may launch a new wave of studies to unveil the relationship between ferroptosis and COVID‐19 and explore more ferroptosis‐related functional food components in daily life for preventing the COVID‐19 and/or to improve the outcome during therapy.
Photocatalyst‐Free Chiral Phosphoric Acid‐Catalyzed Enantioselective Minisci‐Type Alkylation of N‐Heteroarenes
Authors: Jin Songyang , Li Yujun , Wang Rong , Zheng Ke 
Published: September 2024

AbstractIn this study, we present an efficient radical approach for the enantioselective Minisci‐type alkylation of N‐heteroarenes, using chiral phosphoric acid (CPA) as a catalyst under mild conditions, without the need for metals, photocatalysts, and other additives. Prochiral α‐aminoalkyl radicals generated from RAEs were successfully introduced to N‐heteroarene derivatives, including pyridine, quinoline, and isoquinoline, resulting in a range of valuable chiral alkylated N‐heteroarenes with secondary amines in high yields and good to excellent enantioselectivities (up to 98 % ee). The chiral phosphoric acid catalyst not only activates the substrate and induces asymmetry but also facilitates essential electron transfer processes.
A VAMS‐based LC–MS/MS method for precise cenobamate quantification in epilepsy (patients)
Authors: Pigliasco Federica , Cafaro Alessia , Barco Sebastiano , Biondi Margherita , Stella Manuela , Mattioli Francesca , Riva Antonella , de Grazia Ugo , Molteni Linda , Micalizzi Elisa , Villani Flavio , Striano Pasquale , Bandettini Roberto , Cangemi Giuliana 
Published: September 2024

AbstractObjectiveCenobamate (CNB), a recently approved antiseizure medication by the European Medicines Agency (EMA), serves as an adjunctive therapy for focal‐onset seizures in adult patients unresponsive to at least two other treatments. Administered in polytherapy, CNB can potentially interact with co‐administered drugs in epilepsy patients, necessitating dose adjustments and the need for effective therapeutic drug monitoring (TDM).MethodsIn this study, we introduce a novel LC–MS/MS method for precise CNB quantification using Volumetric Absorptive Microsampling (VAMS), following validation according to ICH guidelines M10. VAMS samples are efficiently extracted with 200 μL of methanol, with chromatographic separation achieved using an Acquity UPLC HSS PFP column. The method's efficacy was confirmed through its application to real samples from adult CNB‐treated patients.ResultsOur results demonstrate that the method exhibits linearity within the range of 0.05–30 mg/L, with intra‐ and inter‐run precision ranging from 1% to 8% and accuracy from 1% to 10% based on 30 μL of sample. Furthermore, CNB stability in VAMS is confirmed for up to 15 days at 25°C and −20°C. Importantly, no significant difference was observed between CNB concentrations in VAMS samples and those in plasma obtained from venous blood.SignificanceThis VAMS‐based LC–MS/MS method presents a robust alternative for TDM in CNB‐treated patients. Future investigations should explore CNB concentrations in capillary blood and assess their correlation with plasma levels to further enhance its clinical utility.Plain Language SummaryCenobamate is an antiepileptic drug and used for treatment of focal‐onset seizures in adult patients (≥18 age). TDM can prevent drug interactions and minimize drug toxicity. The aim of this work is to evaluate volumetric absorptive microsampling (VAMS) from capillary blood as an alternative strategy for TDM in patients treated with the newly antiepileptic drug. Our method is suitable for TDM, and this study suggests that VAMS allows monitoring of cenobamate concentration and can offer valuable support for personalized therapy in refractory epilepsy.
Structural and in vitro anticancer properties of the kaempferol–lactoferrin complex
Authors: Xue Peiyu , Zhao Hongmei , You Xinyong , Yue Weiming 
Published: September 2024

AbstractLactoferrin and polyphenols are common natural functional compounds. Their interactions and the consequential alterations in functional activity have received widespread attention. The work aimed to investigate the interaction between lactoferrin and kaempferol, as well as evaluate the in vitro anticancer properties of the lactoferrin–kaempferol complex. The results of the spectra experiments revealed that lactoferrin and kaempferol are capable of forming complexes to quench the endogenous fluorescence of lactoferrin. Further insight into the binding mechanism was gained through molecular docking and molecular dynamics simulations. These analyses suggest that both hydrophobic interactions and hydrogen bonding are essential factors in the interaction between lactoferrin and kaempferol. Furthermore, the MTT assay and apoptosis by flow cytometry were conducted, revealing a synergistic effect of kaempferol and lactoferrin on the inhibition of HeLa cell proliferation. The findings from this investigation could improve our understanding of lactoferrin's interaction with polyphenols and the role of the lactoferrin–kaempferol complex in inhibiting cancer cell proliferation.
Adherence to the Mediterranean diet in patients with psoriasis and its relationship with the severity of the disease: A case‐control study
Authors: Aryanian Zeinab , Asghari Mohsen , Zanousi Parisa Pasha , Ghadimi Reza , Kebria Azar Shirzadian , Hatami Parvaneh 
Published: September 2024

AbstractBackground and AimPsoriasis is a prevalent chronic inflammatory skin condition, and the Mediterranean diet is often recommended for its health benefits, particularly its ability to mitigate chronic inflammation. This study sought to examine the extent to which psoriasis patients adhere to the Mediterranean diet and to explore its correlation with the severity of their conditionMethodsSeventy‐one psoriasis patients and 71 age‐ and sex‐matched healthy controls were enrolled the study and filled a standard questionnaire of adherence to the Mediterranean diet. The relationship between disease severity and adherence to the diet was also dealt with.ResultsThe Mediterranean diet adherence score in the psoriasis group (5.25 ± 1.64) was significantly lower than the control group (6.28 ± 2.10) (p = 0.004). In addition, the consumption of fruit and fish in psoriasis patients was significantly lower than the control group and the consumption of red meat was significantly higher in the patient group. No significant relationship was found between the severity of the disease and the score of adherence to the Mediterranean diet (p = 0.42).ConclusionA significant difference between the two groups of psoriasis and the control group following the Mediterranean diet might be indicative of the relationship between diet and psoriasis and the potential benefits of this type of diet due to its anti‐inflammatory properties.
The effect of an educational video on the immediate insertion of postpartum contraceptive implants: A randomized controlled trial
Authors: Boontor Nutruja , Kaewrudee Srinaree , Sothornwit Jen 
Published: September 2024

AbstractObjectiveTo assess the impact of an educational video on immediate postpartum contraceptive implant utilization.MethodsThis was a randomized controlled study conducted in a university hospital. Postpartum women aged over 18 years were recruited and divided into two groups: the intervention group, which viewed a 7‐min educational video about contraceptive implants; and the control group, which did not. We evaluated the uptake of contraceptive implants immediately and during a 12‐week period postpartum, in addition to the reasons for not selecting this method.ResultsA total of 202 participants were included in the study, 101 in each group. Viewing the educational video was associated with higher immediate postpartum contraceptive implant usage (22.77% vs 10.89%; relative risk [RR] 2.09, 95% confidence interval [CI] 1.08–4.06). However, no significant difference was observed at the postpartum follow‐up visit (29.9% vs 25.74%; RR 1.61, 95% CI 0.74–1.82). The primary reasons for not selecting contraceptive implants were concerns about potential side effects and discomfort associated with the insertion procedure.ConclusionThe inclusion of an animated educational video significantly improved immediate postpartum contraceptive implant uptake, making it a potentially viable strategy in settings with high rates of loss to follow‐up. However, further research into how to address patients' fears regarding the implant is required.
Introduction of advanced maternal age based on mortality‐specific severe morbidity
Authors: Masjedi Aaron D. , Anderson Zachary S. , Pon Fay F. , Matsuzaki Shinya , Mandelbaum Rachel S. , Ouzounian Joseph G. , Matsuo Koji 
Published: September 2024

SynopsisRe‐defining advanced maternal age based on the risk of severe maternal morbidity at delivery may be of value in obstetric medicine.
Differences in the physiology of Pichia pastoris grown on glycerol/glucose using polyurethane foam slabs as solid support
Authors: Zárate‐Bonilla Josué , Rodríguez‐Gómez Divanery , Viniegra‐González Gustavo , López‐Pérez Marcos 
Published: September 2024

AbstractBACKGROUNDGrowth physiology and metabolic flux of Pichia pastoris were analyzed using glass columns containing slabs of polyurethane foam as a solid support and culture medium with glycerol or glucose as a carbon source.RESULTSMedia with glycerol produced an average of 30.91% more biomass than media where glucose was used. However, the specific growth rate registered higher values for media with glucose than those with glycerol at low substrate concentrations: 0.46 versus 0.22 h−1. Media with glycerol produced a large amount of d‐arabitol, whereas ethanol and citrate were the predominant metabolites in media with glucose. Both types of culture systems generated biphasic curves in terms of the respiratory quotient value, whose inflection point coincided with the transition from aerobic metabolism to a mixed one.CONCLUSIONAdequate oxygen transfer occurred in both culture systems; therefore, the differences found in growth physiology can be explained by the transport mechanism that internalizes glycerol, the access point to primary metabolism, and the degree of glycerol oxidation. The arrangement of cells in the polyurethane matrix allowed an attenuation of the repression phenomenon due to excess substrate for both carbon sources. This is the first work to study metabolic flux comparisons using this system. © 2024 Society of Chemical Industry (SCI).
The application value of prenatal ultrasound in conjoined twins
Authors: Xiao Juhua , Liao Kun , Tan Zhiwen , Xie Jiping , Lai Hua , Zhang Shouhua , Luo Ye 
Published: September 2024

AbstractObjectiveTo evaluate the application and effectiveness of prenatal ultrasound in diagnosing and managing conjoined twins.MethodsA retrospective analysis was conducted on 20 cases of conjoined twins diagnosed at our hospital between January 2016 and December 2022. The types of conjoined twins, ultrasonographic characteristics, and associated anomalies were assessed.ResultsThe gestational age at diagnosis ranged from 10 to 35 weeks, with an average of 14.21 ± 5.69 weeks. Thirteen cases were detected in the first trimester, five in the early second trimester, one at 23 + 2 weeks, and one at 35 weeks. Thoracopagus was the most common type (11 cases, 55%), followed by omphalopagus (4 cases, 20%), cephalopagus (4 cases, 20%), and parapagus dicephalus (1 case, 5%). In the first trimester, the most common abnormalities observed included increased nuchal translucency (NT), cystic hygroma, hydrops fetalis, and generalized edema. Major birth defects identified in conjoined twins were omphalocele (3 cases), congenital heart malformations (3 cases), neural tube defects (2 cases), urachal cyst (1 case), and umbilical cyst (1 case). Pregnancy was terminated in 18 cases, one case resulted in spontaneous abortion during the second trimester, and one case was delivered by cesarean section at 37 weeks, with successful separation and recovery.ConclusionPrenatal ultrasound is the primary diagnostic tool for conjoined twins. It effectively assesses the extent of twin fusion, provides critical information for clinical decision‐making, and aids in the management of obstetric care.
Finite element analysis part 1 of 2: Influence of short stem implant polyethylene configuration on glenohumeral joint biomechanics
Authors: Nourissat Geoffroy , Housset Victor , Daudet Jean‐Marie , Fradet Léo , Bianco Rohan‐Jean , Srikumaran Uma 
Published: September 2024

AbstractPurposeStress shielding in short‐stem arthroplasty can cause critical metaphyseal bone loss. If the size and shape of the humeral shaft are important factors, it is unknown whether the shape of the polyethylene component in reverse shoulder arthroplasty (RSA) affects bone stress around or within the stem. We explored the impact of polyethylene shape on humeral and scapular stress distribution using a finite element model.MethodsWe developed a shoulder‐specific finite element model. A defined set of muscle forces was applied to simulate movements. An intact rotator cuff state and a superior deficient rotator cuff state were modelled. We used the FX V135 short stem in three conditions: total shoulder arthroplasty (TSA), and RSA with symmetrical and asymmetrical polyethylene (145°/135°). We measured biomechanical markers related to bone stress for different implant sizes. Joint kinematics and the mechanical behaviour of the implant were compared.ResultsRupture of the supraspinatus muscle produced a functionally limited shoulder. The placement of an anatomic TSA with an intact rotator cuff restored function similar to that of a healthy shoulder. RSA in the rotator cuff‐deficient shoulder restored function regardless of stem size and polyethylene shape. While stem size had an impact on the stress distribution in the bone and implant, it did not show significant potential for increasing or decreasing overall stress. For the same stem, stress distribution at the humerus is different between TSA and RSA. Polyethylene shape did not alter the transmission of stress to the bone in RSA. Asymmetric polyethylene produced a greater abduction range of motion.ConclusionsIn terms of bone stress distribution, smaller stems seemed more appropriate for TSA, while larger stems may be more appropriate for RSA. Polyethylene shape resulted in different ranges of motion but did not influence bone stress.Level of EvidenceDiagnostic Tests or Criteria; Level IV.
A qualitative descriptive study examining the impact of child‐raising experience on Japanese family doctors
Authors: Fujihara Manabu , Watanabe Satoko , Kodama Kuniko , Nakamura Koki , Stanyon Maham , Kanke Satoshi , Kassai Ryuki 
Published: September 2024

AbstractBackgroundThe Patient‐Centered Clinical Method (PCCM) plays an important role in family doctors' ability to communicate effectively with patients. Although the parenting experiences of healthcare providers can be utilized to communicate with patients, it is unclear how they contribute to family doctors' practice.MethodsA qualitative descriptive study focusing on the parenting experiences of Japanese family doctors and their perceptions of how these experiences interfaced with their clinical practice of the PCCM was conducted. Participants were selected using snowball sampling, and the data were collected via semi‐structured interviews while recording online, which were transcribed verbatim and analyzed using qualitative descriptive analysis, with data‐derived codes being systematically applied and reflexively interpreted. The categories were refined concurrently with the interviews. Once it was established that no additional categories were required, the recruitment and analysis process was concluded.ResultsThe eight participants were recruited, and the following themes were extracted; these were “Developing patience and new perspectives through raising children,” “Empathizing with the experiences of others,” “Building awareness of social factors and home context,” “Reflecting on one's own words and behavior,” “Forming partnerships with patients through shared parenting experiences,” and “Facilitating interpersonal growth.”ConclusionThe communication skills that have been refined through personal experiences of raising children are transferable between professional and family situations. This research shows that such skills are not mutually exclusive to professional or family settings and can be used effectively to improve clinical practice as part of the PCCM model of consulting.
d $d$‐connectivity of the random graph with restricted budget
Authors: Lichev Lyuben 
Published: September 2024

AbstractIn this short note, we consider a graph process recently introduced by Frieze, Krivelevich and Michaeli. In their model, the edges of the complete graph are ordered uniformly at random and are then revealed consecutively to a player called Builder. At every round, Builder must decide if they accept the edge proposed at this round or not. We prove that, for every , Builder can construct a spanning ‐connected graph after rounds by accepting edges with probability converging to 1 as . This settles a conjecture of Frieze, Krivelevich and Michaeli.
A Top Solvent‐Additive‐Ionization Technique Combination for Pesticides Direct Infusion MS Analysis
Authors: Anđelković Darko , Branković Milica 
Published: September 2024

ABSTRACTThe leading type of ionization technique in mass spectrometry analyses is the ionization at atmospheric pressure. The aim of this study was to assess ESI and APCI ionization efficiency of pesticides introduced to the MS source in four organic phases, non‐doped and doped with formic acid and ammonium formate. Ionization efficiency in modified ESI and APCI, applying in‐source sample heating, was also assessed. The study was primarily designed to support non‐chromatography‐based mass spectrometry pesticides analysis by the direct infusion technique. Evaluation of analysis performances such as calibration performances, detectability, and sensitivity should indicate a top solvent‐additive‐source combination, leading to the highest ionization efficiency and lowest analytes detection limits.
Cantharidin (1%), podophyllin (5%), and salicylic acid (30%) formulation in recalcitrant plantar warts: Analysis of 48 patients
Authors: Amo‐Navarrete Jéssica , García‐Oreja Sara , León‐Herce Diego , Navarro‐Pérez David , Lázaro‐Martínez José Luís , Álvaro‐Afonso Francisco Javier 
Published: September 2024

AbstractA plantar wart is a benign hyperplasia that appears on the feet due to the human papillomavirus (HPV). One method used for the treatment of recalcitrant plantar warts, those lasting over 2 years or persisting after at least two treatment attempts, is the cantharidin (1%), podophyllin (5%), and salicylic acid (30%) formulation, also known as the CPS formulation. Although this method is in use, there are few studies on it. This study's objective was to ascertain its cure rate. For this retrospective observational study, we reviewed the medical records of patients treated with the CPS formulation at a podiatric clinic specializing in plantar wart treatment. Our sample size was 48 subjects. The CPS formulation had a cure rate of 62.5%. Out of the cured patients, 86.67% (26/30) required one or two applications. There was no observable correlation (p > 0.05) between wart resolution and virus biotype, evolution time, patient's morphological and clinical attributes, location, number of warts, or preceding treatments. The CPS formulation presents a relatively high efficacy rate for treating recalcitrant HPV plantar warts. Still, additional studies are necessary to evaluate its safety and efficiency.
Metabolite variations in sulla shoots induced by drought stress
Authors: Troise Antonio Dario , De Pascale Sabrina , Piluzza Giovanna , Re Giovanni Antonio , Sanna Federico , Scaloni Andrea , Sulas Leonardo 
Published: September 2024

AbstractBACKGROUNDClimate change results in an increased occurrence of drought periods, which affect the performance of Mediterranean legumes such as sulla. To evaluate the effects of drought stress on the antioxidant capacity and the accumulation of phytochemicals in sulla shoots, rainfed and drought‐stressed plants (RAI) and watered plants with timely vegetative regeneration (STI) were cultivated in parallel in a field experiment and sampled at four different times.RESULTSDrought stress strongly and positively affected the antioxidant capacity and the accumulation of polyphenols in sulla shoots, with key differences at wintertime. These findings were supported through untargeted and targeted metabolomics of RAI and STI samples, which detailed the accumulation of about 300 secondary metabolites in RAI, mostly polyphenols and saponins.CONCLUSIONThis study provided new insights into the changes in secondary metabolites in sulla due to drought stress, which can be used to produce forages or raw plant extracts with enhanced biological effects. © 2024 Society of Chemical Industry.
Limosilactobacillus reuteri RE225 alleviates gout by modulating the TLR4/MyD88/NF‐κB inflammatory pathway and the Nrf2/HO‐1 oxidative stress pathway, and by regulating gut microbiota
Authors: Qin Yang , Zhou Yucong , Xiong Jiayi , Lu Chenyang , Zhou Jun , Su Xiurong , Han Jiaojiao 
Published: September 2024

AbstractBACKGROUNDGout poses a significant health threat. The use of Lactobacillus from the gut microbiota is one potential remedy. However, the intricate molecular mechanisms governing the impact of Lactobacillus on gout remain largely uncharted. In this study, a strain of Limosilactobacillus reuteri RE225 was separated from the gut of mice and colitis was treated with polypeptide intervention.RESULTSLimosilactobacillus reuteri RE225 reduced foot tumefaction markedly in mice with gout and extended the pain threshold time in their feet. It also improved the health of gut microbiota. Intervention with L. reuteri RE225 also suppressed the TLR4/MyD88/NF‐κB and nuclear factor erythroid 2‐related factor 2 (Nrf2)/heme oxygenase‐1 (HO‐1) pathways in the mice, reduced the levels of pro‐inflammatory cytokines – interleukin 1β (IL‐1β), interleukin 6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) – and increased the level of the anti‐inflammatory cytokine interleukin 10 (IL‐10), thereby mitigating inflammation.CONCLUSIONThis study provides a theoretical basis for the comprehensive development of Limosilactobacillus reuteri and new ideas for the non‐pharmacological treatment of gout. © 2024 Society of Chemical Industry.