bluuebunny/crossref_metadata_2025_split huggingface dataset:
Familial Crisis and Marriage
Authors: Srinivasan* Rama 
Published: October 2024

Abstract
This chapter builds on Arjun Appadurai’s works to contextualize the lives of women from rural North India, who dwell in ‘disjointed ethnoscapes’ and have found the navigational capacity of aspiration. In Haryana and Punjab, which have seen a Green Revolution, land ownership was once a source of family pride and prosperity for male members but cannot ensure steady income and local dominance today. Building upon her previous work, the author argues that the young rural women’s aspirations for education and employment stems from this agrarian crisis where women’s unrecognized labour in family-held land for the enrichment of their male relatives is no longer mandated. This is also reflected in the emerging possibilities of non-normative and even intercaste marriages. This chapter thus highlights how the navigational capacity of women and a corresponding crisis in masculinity is both the source and product of a family crisis in post-agrarian India.
Spilt Blood
Authors: Haripriya Soibam 
Published: October 2024

Abstract
This chapter argues that sites of violence reveal different calibrations of intimate relationships—whether familial or that of friendship. Contexts of protracted violence inflect kinship and friendship in ways that make negotiation with trust and scepticism foundational to such relationships. Such relationships are unable to escape the state/non-state scrutiny and the anxious impulse of categorizing people as collaborators, state/non-state agents, or sympathizers of a particular group. The chapter explores family relationships and friendship in the militarized context of Manipur to enquire how care and affection practices are negotiated in the face of social dismemberment. Taking recourse to fiction and anecdotes, the author attempts to construct the field by drawing attention to the predicament of living in violent places that are under the gaze of both the state and the non-state armed groups.
Combined programmed cell death protein 1 and cytotoxic T-lymphocyte associated protein 4 blockade in an international cohort of patients with acral lentiginous melanoma
Authors: McGillivray Erin , Ashouri Karam , Chatziioannou Eftychia , Gallegos Jesús Antonio Ocejo , Zarka Jabra , Kechter Jacob , Hwang Angelina S , Zhang Kevin , Barros Milton , Yeh Justin , Okazaki Ian , Crocker Andrew B , Maeda Takuya , Park Soo J , Choi Jacob , Andreoli Mia , Darwish Tarneem , Savage David J , Kim Kevin B , Gupta Jayant , Shen James , Shirai Keisuke , Choi April , Pai Lori , Vazquez Vinicius de Lima , Moser Justin , Amaral Teresa , Hernandez Aya Leonel F , Lutzky Jose , Najjar Yana G , Costello Collin M , Mangold Aaron R , Bhatia Shailender , Gibney Geoffrey T , Farma Jeffrey M , Daniels Gregory A , Sosman Jeffrey , Chandra Sunandana , Mangla Ankit , Bollin Kathryn , Possik Patrícia Abrão , Robles-Espinoza Carla Daniela , Ito Fumito , In Gino K 
Published: October 2024

Abstract

Background
Combination immune checkpoint blockade targeting programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte associated protein 4 (CTLA-4) leads to high response rates and improved survival in patients with advanced cutaneous melanoma (CM). Less is known about the efficacy of this combination in acral lentiginous melanoma (ALM).

Objectives
To determine the efficacy of combination immune checkpoint blockade targeting PD-1 and CTLA-4 in a diverse, real-world population of patients with ALM.

Methods
This multi-institutional retrospective study analysed patients with histologically confirmed ALM treated with a combination of PD-1 and CTLA-4 inhibitors between 2010 and 2022. The primary objective of the study was the objective response rate (ORR) as per the RECIST criteria. The secondary objectives were progression-free survival (PFS) and overall survival (OS).

Results
In total, 109 patients with advanced ALM treated with combined PD-1 and CTLA-4 blockade in any line of treatment were included. The majority of patients had stage IV disease (n = 81; 74.3%). The ORR for the entire cohort was 18.3% [95% confidence interval (CI) 11.6–26.9], with 9 (8.3%) complete and 11 (10.1%) partial responses. A further 22 patients (20.2%) had stable disease, and the disease control rate was 38.5%. Median PFS was 4.2 months (95% CI 3.25–5.62), while median OS was 17 months (95% CI 12.4–23.1). Ninety-five patients (87.2%) had a treatment-related adverse event, with 40.4% (n = 44/109) experiencing at least one grade 3 or 4 toxicity. Elevated lactate dehydrogenase (P = 0.04), ≥ 2 lines of prior treatment (P = 0.03) and Asian ethnicity (P = 0.04) were associated with worse OS, while Hispanic/Latino ethnicity was associated with better OS (P = 0.02).

Conclusions
Combination PD-1 and CTLA-4 blockade is less effective for ALM than for CM, despite similar toxicity. In particular, Asian patients appear to derive less benefit from this regimen. Novel treatment approaches are needed for this rare melanoma subtype.
Efficacy and safety of the ustekinumab biosimilar ABP 654 in patients with moderate-to-severe plaque psoriasis: a randomized, double-blinded, active-controlled, comparative clinical study over 52 weeks
Authors: Blauvelt Andrew , Papp Kim , Trivedi Mona , Barragan Carolina , Chow Vincent , Mytych Daniel T , Yamauchi Paul , Crowley Jeff , Franklin Janet 
Published: October 2024

Abstract

Background
ABP 654 is a biosimilar to ustekinumab reference product (RP). ABP 654 has been shown to have an amino acid sequence identical to ustekinumab RP and they are similar in structure, purity, and potency as well as clinical pharmacokinetics and safety in healthy volunteers.

Objectives
This randomized, double-blinded, active-controlled, single-transition, comparative clinical study (ClinicalTrials.gov ID NCT04607980) was conducted to compare the efficacy, safety, and immunogenicity of ABP 654 and ustekinumab RP in patients with moderate-to-severe plaque psoriasis.

Methods
Patients were randomized in a 1:1 ratio to receive ABP 654 or ustekinumab RP at a weight-based dose of 45 mg or 90 mg administered subcutaneously on day 1 (week 0), week 4, and week 16. At week 28, patients with a ≥75% improvement in Psoriasis Area and Severity Index (PASI) were re-randomized such that those initially randomized to ABP 654 continued to receive ABP 654 and those initially randomized to ustekinumab RP were re-randomized to either continue on ustekinumab RP or transition to ABP 654. The primary efficacy endpoint was PASI percent improvement from baseline to week 12. Secondary endpoints included additional efficacy measurements as well as an assessment of adverse events and antidrug antibodies.

Results
At week 12, the observed mean (SD) PASI percent improvement from baseline was 81.9 (19.9) and 81.9 (19.6) for the ABP 654 and ustekinumab RP treatment groups, respectively. The point estimate of the mean difference in PASI percent improvement from baseline to week 12 between the treatment groups was 0.14 with a 2-sided 90% confidence interval (CI) of (-2.6, 2.9), well within the prespecified similarity margin of (-10, +10). In addition, throughout the study, secondary efficacy analyses and safety and immunogenicity profiles were similar across the treatment groups.

Conclusions
These results indicate that ABP 654 and ustekinumab RP are clinically similar in efficacy, safety, and immunogenicity in patients with moderate-to-severe plaque psoriasis. Further, a single transition from ustekinumab RP to ABP 654 at week 28 had no impact on the efficacy, safety, or immunogenicity results for the remainder of the 52-week study, supporting a conclusion of no clinically meaningful differences between ABP 654 and ustekinumab RP.
Subject-based assessment of large-scale integration dynamics in epileptic brain networks: insights from the intrinsic ignition framework
Authors: Donaire Antonio , Padilla Nelly , Escrichs Anira , Khawja Mariam , Setoain Xavier , Rumia Jordi , Roldan Pedro , Bargallo Nuria , Boget Teresa , Pintor Luis , Centeno María , Conde Estefanía , Vernet Oriol , Buendía Javier , Manzanares Isabel , Ådén Ulrika , Carreño Mar , Kringelbach Morten , Deco Gustavo 
Published: October 2024

Abstract
This study examined the dynamic properties of brain regions involved in the genesis and spread of seizures in 10 individuals diagnosed with pharmacoresistant focal epilepsy. The patients and 30 healthy controls underwent resting-state functional magnetic resonance imaging scans and the brain’s functional network dynamics were analyzed using the intrinsic ignition framework. Comparative statistical analyses examined the differences in the integration and metastability measures in both groups in the whole brain and specific local brain regions. Invasive electroencephalography evaluations validated the findings of significant global and regional changes in the patient’s brain network dynamics. There was a marked increase in global integration and metastability across the brain, reflecting substantial alterations in the overall connectivity and flexibility of the functional networks. Specific brain regions exhibited paradoxical dynamics within the seizure onset zone, with decreased intrinsic ignition and increased metastability. Increased intrinsic ignition was observed in remote brain regions, suggesting a reorganization of the brain network hubs and potential pathways for seizure propagation. Using the intrinsic ignition framework provided insights into dynamic alterations in the brain networks of patients with epilepsy. These have increased our understanding of the mechanisms underlying epileptic seizures and may guide the development of diagnostic biomarkers and targeted therapeutic interventions.
Simultaneous versus staged approach in transcatheter aortic valve implantation for severe stenosis and endovascular aortic repair for thoracic and abdominal aortic aneurysm
Authors: Gallitto Enrico , Spath Paolo , Faggioli Gian Luca , Saia Francesco , Palmerini Tullio , Piazza Michele , D’Oria Mario , Simonte Gioele , Cappiello Antonio , Isernia Giacomo , Gelpi Guido , Rizza Antonio , Piffaretti Gabriele , Gargiulo Mauro ,   the Italian Multicenter T/EVAR + TAVI Study’s Group, Antonello M , Bellosta R , Berti S , Bramucci A , Cappiello A , Cecere F , Di Marzo L , D’Oria M , Faggioli G L , Freyrie A , Gallitto E , Gargiulo M , Gelpi G , Gennai S , Isernia G , Lepidi S , Lodato M , Marrozzini C , Palmerini T , Pratesi G , Piazza M , Mansour W , Mezzetto L , Piffaretti G , Rizza A , Saia F , Silingardi R , Simonte G , Squizzato F , Spath P , Tinelli G , Tozzi M , Trimarchi S , Veraldi G F 
Published: October 2024

Abstract

OBJECTIVES
Thoracic/abdominal aortic aneurysms and aortic stenosis may be concomitant diseases requiring both transcatheter aortic valve implantation (TAVI) and endovascular aneurysm repair (T/EVAR) in high-risk patients for surgical approaches, but temporal management is not clearly defined yet. The aim of the study was to analyse outcomes of simultaneous versus staged TAVI and T/EVAR.

METHODS
Retrospective observational multicentre study was performed on patients requiring TAVI and T/EVAR from 2016 to 2022. Patients were divided into 2 groups: ‘Simultaneous group’ if T/EVAR + TAVI were performed in the same procedure and ‘Staged group’ if T/EVAR and TAVI were performed in 2 steps, but within 3 months. Primary outcomes were technical success, 30-day mortality/major adverse events and follow-up survival. Secondary outcomes were procedural metrics and length of stay.

RESULTS
Forty-four cases were collected; 8 (18%) had T/EVAR and 36 (82%) had EVAR, respectively. Upon temporal determination, 25 (57%) and 19 (43%) were clustered in Simultaneous and Staged groups, respectively. In Staged group, median time between procedures was 72 (interquartile range—IQR: 57–87) days. Preoperative and intraoperative figures were similar. There was no difference in 30-day mortality (Simultaneous: 0/25 versus Staged: 1/19; P = 0.43). Pulmonary events (Simultaneous: 0/25 versus Staged: 5/19; P = 0.01) and need of postoperative cardiac pacemaker (Simultaneous: 2/25 versus Staged: 7/19; P = 0.02) were more frequent in Staged patients. The overall length of stay was lower in the Simultaneous group [Simultaneous: 7 (IQR: 6–8) versus Staged: 19 (IQR: 15–23) days; P = 0.001]. The median follow-up was 25 (IQR: 8–42) months and estimated 3-year survival was 73% with no difference between groups (Simultaneous: 82% versus Staged: 74%; P = 0.90).

CONCLUSIONS
Both Simultaneous or Staged T/EVAR and TAVI procedures are effective with satisfactory outcomes. Despite the small numbers, simultaneous repair seems to reduce length of stay and pulmonary complications, maintaining similar follow-up survival.
Whole-genome-based taxonomy as the most accurate approach to identify Flavobacterium species
Authors: Gélinas Vincent , Paquet Valérie E , Paquet Maude F , Vincent Antony T , Charette Steve J 
Published: October 2024

Abstract
The genus Flavobacterium comprises a diversity of species, including fish pathogens. Multiple techniques have been used to identify isolates of this genus, such as phenotyping, polymerase chain reaction genotyping, and in silico whole-genome taxonomy. In this study, we demonstrate that whole-genome-based taxonomy, using average nucleotide identity and molecular phylogeny, is the most accurate approach for Flavobacterium species. We obtained various isolated strains from official collections; these strains had been previously characterized by a third party using various identification methodologies. We analyzed isolates by PCR genotyping using previously published primers targeting gyrB and gyrA genes, which are supposedly specific to the genus Flavobacterium and Flavobacterium psychrophilum, respectively. After genomic analysis, nearly half of the isolates had their identities re-evaluated: around a quarter of them were re-assigned to other genera and two isolates are new species of flavobacteria. In retrospect, the phenotyping method was the least accurate. While gyrB genotyping was accurate with the isolates included in this study, bioinformatics analysis suggests that only 70% of the Flavobacterium species could be appropriately identified using this approach. We propose that whole-genome taxonomy should be used for accurate Flavobacterium identification, and we encourage bacterial collections to review the identification of isolates identified by phenotyping.
Enhancing CRISPR-Cas-based gene targeting in tomato using a dominant-negative ku80
Authors: Vu Tien Van , Nguyen Ngan Thi , Kim Jihae , Vu Minh Huy , Song Young Jong , Tran Mil Thi , Sung Yeon Woo , Kim Jae-Yean 
Published: October 2024

Abstract
The CRISPR-Cas-based gene targeting (GT) method has enabled precise modifications of genomic DNA ranging from single base to several kilobase scales through homologous recombination (HR). In plant somatic cells, canonical non-homologous end-joining (cNHEJ) is the predominant mechanism for repairing double-stranded breaks (DSBs), thus limiting the HR-mediated GT. In this study, we implemented an approach to shift the repair pathway preference toward HR by using a dominant-negative ku80 mutant protein (KUDN) to disrupt the initiation of cNHEJ. The employment of KUDN conferred a 1.71- to 3.55-fold improvement in GT efficiency at the callus stage. When we screened transformants, there was a more remarkable increase in GT efficiency, ranging from 1.62- to 9.84-fold, at two specific tomato loci, SlHKT1;2 and SlEPSPS1. With practical levels of efficiency, this enhanced KUDN-based GT tool successfully facilitated a 9-bp addition at an additional locus, SlCAB13. These findings provide another promising method for more efficient and precise plant breeding.
Preparation of Material for Compiling an English–Slovene Dictionary of Criminal Justice and Security Collocations
Authors: Lukančič Mojca Kompara 
Published: October 2024

Abstract
This article presents the preparation of material for compiling an English–Slovene dictionary of criminal justice and security collocations. It presents the process of collecting and preparing material; specifically, software selection, corpus preparation, collocation extraction, and translation into Slovene. These activities were performed by students at the University of Maribor’s Faculty of Criminal Justice and Security as part of the course English Terminology. It defines the concepts of language for specific purposes (LSP) and presents the field and subfields of criminal justice and security, and gives an overview of the definitions of collocations. The article outlines the need to prepare a collocation dictionary by exploring the inclusion of collocations in dictionaries. The characteristics of possible dictionary entries are outlined based on material collected and prepared by the students. This is followed by the presentation of some examples of collocation entries from dictionaries.
Review: Current Laboratory and Point-of-Care Pharyngitis Diagnostic Testing and Knowledge Gaps
Authors: Boyanton Bobby L , Caldwell Jane M , Ledeboer Nathan A 
Published: October 2024

Abstract
Pharyngitis is an inflammatory condition of the pharynx and/or tonsils commonly seen in both children and adults. Viruses and bacteria represent the most common encountered etiologic agents—yeast/fungi and parasites are infrequently implicated. Some of these are predominantly observed in unique populations (eg, immunocompromised or unvaccinated individuals). This manuscript (part 2 of 3) summarizes the current state of laboratory and point-of-care diagnostic testing and highlights the expanding role of nucleic acid amplification in the expedited diagnosis and management of patients with acute pharyngitis. It discusses preanalytical, analytical, and postanalytical variables that impact the performance of culture, rapid antigen, and nucleic acid amplification testing. Finally, it sets the stage for part 3, which discusses the emerging role of biomarkers in the management of individuals with acute pharyngitis.
An Expression Quantitative Trait Locus of Fc Gamma Receptor Genes Is Associated With Antimalarial IgG Responses and Infection Levels in Burkinabe Families
Authors: Dieppois Christelle , Adjemout Mathieu , Cretin Jules , Gallardo Frederic , Torres Magali , Picard Christophe , Sawadogo Serge Aimé , Rihet Pascal , Paul Pascale 
Published: October 2024

Abstract

Background
The interaction between antibodies and Fcγ receptors (FcγRs) plays a critical role in regulating immune responses to Plasmodium falciparum. Polymorphisms in genes encoding FcγRs influence the host's capacity to control parasite infection. This study investigates whether noncoding variants influencing FcγR expression are associated with antimalarial immunization and infection traits.

Methods
We utilized eQTL databases and functional annotations to identify noncoding variants, specifically rs1771575, rs2099684, and rs6700241, within the FCGR gene cluster. In addition, we examined the coding variants rs1801274 (p.His167Arg) and rs1050501 (p.Ile231Thr), which affect the affinity of FcγRIIa and FcγRIIb for IgG. These variants were genotyped in 163 individuals from Burkinabe families. Family-based linear mixed regression and Quantitative Transmission Disequilibrium Tests (QTDT) analyses were performed to assess associations with IgG levels and malaria infection, accounting for relevant covariates.

Results
Linear mixed models identified rs1771575 as associated with total IgG levels, while both rs1771575 and rs1801274 were linked to IgG2, and rs1050501 to IgG1 levels. A haplotype combining rs2099684 and rs6700241 was positively associated with IgG1. The rs1771575-CC and rs1050501-TT genotypes correlated with higher infection levels in children. QTDT models confirmed the association of rs1771575 with IgG2 and infection in children.

Conclusions
Our findings suggest that the intergenic variant rs1771575 serves as an independent marker for IgG levels and blood infection in children. This highlights the interplay between regulatory variants and coding mutations in FCGR, which may influence immune function and antibody production. These results underscore the potential for personalized strategies to monitor humoral responses in malaria-endemic regions.
Whole-genome assembly and annotation of the firecracker penstemon (Penstemon eatonii)
Authors: Jarvis David E , Stevens Mikel R , Carter Payton , Lin Ying Fei , Jaggi Kate E , Jijon Gabriela , Kalt Teal , Calixto Jouber , Standring Samantha , Torres Kaitlin , Stephensen Kayla B , Mangelson Hayley , Williams Noah H , Wessinger Carolyn A , Maughan Peter J , Frandsen Paul B 
Published: October 2024

Abstract
The penstemons are ornamental annual flowering plants native to the Intermountain West and Rocky Mountains and commonly used for urban landscaping. Elite commercial penstemons are generally susceptible to abiotic stresses, including drought, root rot, cold, and high salinity. Firecracker penstemon (Penstemon eatonii), however, is much more tolerant to these stresses than most elite cultivars. Importantly, firecracker penstemon has been reported to hybridize with many other penstemons and therefore provides the opportunity to develop more tolerant elite cultivars through strategic crossing. To facilitate the study and utilization of firecracker penstemon, we sequenced and annotated the genome of a P. eatonii accession collected from Utah, United States. We also performed low-coverage whole-genome sequencing of 26 additional accessions from three different varieties of P. eatonii. This chromosome-scale genome assembly is the most contiguous and complete Penstemon genome sequenced to date.