bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted content dissemination in mobile social networks taking account of resource limitation
Authors: Ravaei Bahman , Sabaei Masoud , Pedram Hossein , Valaee Shahrokh 
Published: July 2017

SummaryMobile social networks are presenting new opportunities for content dissemination. User location, mobility, and social communities can be used to deliver delay‐tolerant content. Most existing dissemination methods either fail to consider the user's interest in their protocol design or just allow exchange of contents between nodes that have a similar interest. If nodes with similar interest never encounter each other, then contents might not be exchanged with them. In this paper, we propose a method in which user location, mobility, and interest as well as certain limiting factors such as relay buffer size and communication overhead are used to select a set of relays for targeted advertisement distribution. Besides, our method does not depend on nodes with similar interest encountering one another. In the proposed method, a distribution agent exploits user location, mobility, and social networks as well as the interest of destinations to select a group of relays to carry content to the targeted destinations. Users move among social communities and carry advertisements to their peers in other communities. We have also developed an optimization problem for advertisement selection and scheduling. Since the problem is NP‐hard, we propose a heuristic solution. Evaluation of the proposed approach shows that the algorithm is not sensitive to network resource variation and readily outperforms popular methods reported in the literature in terms of delay, delivery ratio, and interest compatibility.
Maximum linear matching: Intelligent and automatic wavelength calibration method
Authors: Yang Lu , Song Qing , Wang Zhihui , Zhang Shihui 
Published: July 2017

SummaryWavelength calibration is a necessary means to ensure the normal operation of the spectrometer. In general, calibration light sources that can emit fixed wavelength (such as mercury‐argon lamp) are utilized to generate spectral line on the linear array charge‐coupled device detector. Thus, it is a prerequisite for wavelength calibration to match the pixel position of the well‐divided spectral line with light of different wavelength emitted by calibration light source correctly. In this paper, we aim to present a method to make calibration procedure intelligent and automatic by machine. We establish a pixel‐wavelength model based on bipartite graph and propose the maximum linear matching (MLM) algorithm to find the correct set of pixel‐wavelength automatically. Meanwhile, we calculate precision and recall to measure the effectiveness of MLM and analyze the practical application of MLM by comparing it with conventional artificial methods. Experiments show that the autocalibration method based on MLM can calibrate many types of grating spectrometer more accurately and more reliably. With MLM, we report 98.33% precision and 94.59% recall on 8 groups of experiments.
Genesis of a Co–Salicylaldimine Complex on Silica Followed in Situ by FTIR and XAS
Authors: Ferri Davide , Gaur Abhijeet , Grunwaldt Jan‐Dierk 
Published: July 2017

AbstractSeveral strategies have been proposed to replace soluble metallorganic complexes in organic solvents by similar molecular entities immobilized on non‐reactive solids. The characterization of these complexes at atomic and molecular level during synthesis is demanding but essential to guide rational design. In the present work, the formation of cobalt salicylaldimine complex on γ‐aminopropyl modified silica (SiO2) was monitored in ethanol on‐line by Fourier transform infrared spectroscopy (FTIR) and in situ X‐ray absorption spectroscopy (XAS) simultaneously using two independent cells. The organic ligand was monitored by FTIR to follow the stepwise synthesis of the Co–salicylaldimine complex. The oxidation state of Co, obtained by XANES, was found to be +2, while different coordination environments were observed in the presence or absence of the pendant organic ligand produced in situ on SiO2. EXAFS analysis inferred that the oxidation state and the local structure of the Co2+ ion on the modified SiO2 surface was similar to that of a salen‐complex with four Co‐O/N bonds.
Strategies for building a successful ultrasound guided FNA practice in department of pathology—Experience at a university hospital
Authors: Wu Maoxin , Shroyer Kenneth R. 
Published: July 2017

AbstractIntroductionAlthough pathology services focused on fine needle aspiration (FNA) biopsy of superficial palpable masses with on‐site cytological evaluation are available in a wide range of clinical and academic settings, the addition of ultrasound (US) guidance into FNA practice by a cytopathologist group can be challenging. An US‐FNA service provided by a cytopathologist in the department of Pathology is a relatively new practice in the field of medicine. This report summarizes our experience and strategies in achieving this objective and our successful preliminary results.Materials and MethodsThe US‐FNA service includes (1) an FNA procedure to be performed by a cytopathologist under US guidance; (2) onsite adequacy evaluation and diagnosis to be done by the same cytopathologist; and immediate patient consultation and sample triaging carried out by the same cytopathologist in an FNA suite within the department of Pathology. The FNA suite including a procedure room equipped with a portable US machine, an exam/procedure table, a mobile cabinet with FNA supplies, a counter with sink, and a reception room with waiting area is set‐up.ResultsThe establishment of the US‐FNA service is successful. There is an incremental growth of the service over the first 8 months. Among the 114 cases performed during the first 8 months, the case type distribution is shown to be 50% thyroid nodules, 33% lymph nodes, 5.5% salivary gland masses, 3.5% breast masses, and 8% soft tissue masses.ConclusionsThe authors' initial 8 months experience and strategies in setting up a new US‐FNA practice in a new institution are discussed to highlight obstacles encountered and approaches that promoted the successful establishment of a new service. A conservative approach, focusing on building partnerships with existing clinical services, can be successfully implemented in most institutions, if appropriate strategies are applied. The main strategy is to ensure that the best interests of patients remain the primary focus and that everything possible is done to improve the quality and effectiveness of patient care by providing the best possible diagnostic US‐FNA service, to enable optimal clinical management.
Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA
Authors: Stanković Dalibor M. , Švorc Ľubomir , Mariano José F. M. L. , Ortner Astrid , Kalcher Kurt 
Published: July 2017

AbstractColchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification‐free boron‐doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19 V, and second one at around 1.37 V, in Britton‐Robinson buffer solution at pH 7.5. Wide dynamic range from 1 to 100 μM was obtained with a detection limit (3σintercept/slope) of a 0.26 μM, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time‐consuming and expensive separation methods.
In vitro release and permeation kinetics of Melaleuca alternifolia (tea tree) essential oil bioactive compounds from topical formulations
Authors: Sgorbini Barbara , Cagliero Cecilia , Argenziano Monica , Cavalli Roberta , Bicchi Carlo , Rubiolo Patrizia 
Published: July 2017

AbstractMelaleuca alternifolia essential oil (tea tree oil, TTO) is well known for its antimicrobial, antifungal and anti‐inflammatory properties, which it is currently used as an active ingredient in skin care products. This study investigates and evaluates the permeation and release kinetics of TTO marker compounds from several semisolid formulations (creams, ointments, gels) containing TTO in different percentages (5–30% w/w). In vitro permeation and release tests were run in vertical Franz diffusion cells with synthetic and pig‐skin membranes for different times (from 10 minutes to 50 hours). The recovered receiving phases were analyzed by Headspace Solid‐Phase Microextraction (HS‐SPME) in combination with Gas Chromatography coupled with Mass Spectrometry (GC–MS). The method adopted is completely automatic and provides on‐line monitoring of the release and permeation kinetics, while avoiding time‐consuming solvent extraction. The study examined both the total amount of essential oil and some selected markers known to be responsible for TTO's biological activities, i.e. 1,8‐cineole, 4‐terpineol and α‐terpineol. The results of in vitro release and permeation tests demonstrated that all compounds show the same kinetics profiles, although amount released differs significantly. The markers were quantified by external calibration curves constructed through HS‐SPME‐GC–MS. Some preliminary experiments were also run to monitor the presence of the above markers within the skin.
KIAA1199: A novel regulator of MEK/ERK‐induced Schwann cell dedifferentiation
Authors: Boerboom Angélique , Reusch Céline , Pieltain Alexandra , Chariot Alain , Franzen Rachelle 
Published: July 2017

AbstractThe molecular mechanisms that regulate Schwann cell (SC) plasticity and the role of the Nrg1/ErbB‐induced MEK1/ERK1/2 signalling pathway in SC dedifferentiation or in myelination remain unclear. It is currently believed that different levels of MEK1/ERK1/2 activation define the state of SC differentiation. Thus, the identification of new regulators of MEK1/ERK1/2 signalling could help to decipher the context‐specific aspects driving the effects of this pathway on SC plasticity. In this perspective, we have investigated the potential role of KIAA1199, a protein that promotes ErbB and MEK1/ERK1/2 signalling in cancer cells, in SC plasticity. We depleted KIAA1199 in the SC‐derived MSC80 cell line with RNA‐interference‐based strategy and also generated Tamoxifen‐inducible and conditional mouse models in which KIAA1199 is inactivated through homologous recombination, using the Cre‐lox technology. We show that the invalidation of KIAA1199 in SC decreases the expression of cJun and other negative regulators of myelination and elevates Krox20, driving them towards a pro‐myelinating phenotype. We further show that in dedifferentiation conditions, SC invalidated for KIAA1199 exhibit lower myelin clearance as well as increased myelination capacity. Finally, the Nrg1‐induced activation of the MEK/ERK/1/2 pathway is severely reduced when KIAA1199 is absent, indicating that KIAA1199 promotes Nrg1‐dependent MEK1 and ERK1/2 activation in SCs. In conclusion, this work identifies KIAA1199 as a novel regulator of MEK/ERK‐induced SC dedifferentiation and contributes to a better understanding of the molecular control of SC dedifferentiation.
Standardizing bimanual vaginal examination using cognitive task analysis
Authors: Plumptre Isabella , Mulki Omar , Granados Alejandro , Gayle Claudine , Ahmed Shahla , Low‐Beer Naomi , Higham Jenny , Bello Fernando 
Published: July 2017

AbstractObjectiveTo create a standardized universal list of procedural steps for bimanual vaginal examination (BVE) for teaching, assessment, and simulator development.MethodsThis observational study, conducted from June–July 2012 and July–December 2014, collected video data of 10 expert clinicians performing BVE in a nonclinical environment. Video data were analyzed to produce a cognitive task analysis (CTA) of the examination steps performed. The CTA was further refined through structured interviews to make it suitable for teaching or assessment. It was validated through its use as a procedural examination checklist to rate expert clinician performance.ResultsBVE was deconstructed into 88 detailed steps outlining the complete examination process. These initial 88 steps were reduced to 35 by focusing on the unseen internal examination, then further refined through interviews with five experts into 30 essential procedural steps, five of which are additional steps if pathology is suspected. Using the CTA as a procedural checklist, the mean number of steps performed and/or verbalized was 21.6 ± 3.12 (72% ± 10.4%; range, 15.9–27.9, 53%–93%).ConclusionThis approach identified 30 essential steps for performing BVE, producing a new technique and standardized tool for teaching, assessment, and simulator development.
Biological responses in Balb/c mice after long‐term parenteral administration of the light subunit of mushroom tyrosinase
Authors: Ismaya Wangsa T. , Efthyani Alida , Tjandrawinata Raymond R. , Rachmawati Heni 
Published: July 2017

AbstractLight subunit of mushroom tyrosinase (LSMT) is a protein of unknown function from mushroom Agaricus bisporus that has been demonstrated to permeate through rat intestine ex vivo. Thus, it can be absorbed in the intestine, thereby holding a promise as a drug carrier for oral administration, similar to HA‐33 protein from botulinum, one of the closest structural homologs of LSMT. However, the safety of LSMT should be ensured prior to its use. Here, we described biological response of LSMT upon weekly intraperitoneal administration of 50 μg/day to the Balb/c mice for 12 weeks. Motoric and behavior profiles, as well as the index of main organs (liver, spleen, lung, heart, and kidney), and body weight, were not significantly changed as compared with the control group. Also, no IgG was detected in the serum. The results suggest that LSMT is safe for further development.
Scanning electron and light microscopy of foliar epidermal characters: A tool for plant taxonomists in the identification of grasses
Authors: Khan Raees , Ahmad Mushtaq , Zafar Muhammad , Ullah Asad 
Published: July 2017

AbstractSEM and light microscopy are of special interest for biologist to observe various features of the living bodies. In the current study we observed the foliar epidermal micro‐morphological characters of 44 grass species using SEM and Light microscopy to assess their taxonomic utility for taxonomists in the identification process. The aim of this study is to use the foliar epidermal structural variations in both upper and lower surfaces for identification of grasses. Significant diversity was observed in both qualitative and quantitative characters using SEM and Light microscopy. Variations were observed in stomatal number, size, guard cells shape, silica bodies, macro‐hairs, micro‐hairs, epidermal cell number, subsidiary cells, prickles, hooks, papillae, and short and long cells. A taxonomic key is prepared using these variations for the identification of grass species. Based on these findings, SEM and Light microscopy of foliar epidermal features can be of special interest for taxonomists in the identification of complex grass taxa.
Modeling and simulation of 2DEG density and intrinsic capacitances in AlInN/GaN MOSHEMT
Authors: Amarnath G. , Swain R. , Lenka T.R. 
Published: July 2017

AbstractIn this paper, an analytical model for intrinsic capacitance is developed by estimating 2‐dimensional electron gas density inside the triangular quantum well in AlInN/GaN metal oxide semiconductor high‐electron mobility transistors by considering gate charge and through self‐consistent solution of Poisson and Schrödinger equations. The charge separation method is applied to develop the intrinsic terminal charges and then the intrinsic capacitances accordingly. In order to validate the developed model, the results are compared with TCAD device simulation results.
Different characteristics of cell volume and intracellular calcium ion concentration dynamics between the hippocampal CA1 and lateral cerebral cortex of male mouse brain slices during exposure to hypotonic stress
Authors: Takahashi Nanae , Omi Akibumi , Uchino Hiroyuki , Kudo Yoshihisa 
Published: July 2017

AbstractThe mechanism of brain edema is complex and still remains unclear. Our aim was to investigate the regional differences of cell volume and intracellular Ca2+ concentration ([Ca2+]i) dynamics during hypotonic stress in male mouse hemi‐brain slices. Brain slices were loaded with the fluorescence Ca2+ indicator fura‐2, and cell volume and [Ca2+]i in the lateral cerebral cortex (LCC) and hippocampal CA1 (CA1) region were measured simultaneously during exposure to hypotonic stress using Ca2+ insensitive (F360) and Ca2+ sensitive fluorescence (F380), respectively. Brain cell swelling induced by hypotonic stress was followed by a regulatory volume change that coincided with an increase in [Ca2+]i. The degrees of change in cell volume and [Ca2+]i were significantly different between the LCC and CA1. The increase in cell volume and [Ca2+]i in the LCC, but not in the CA1, was decreased by the transient receptor potential channel blockers LaCl3 and GdCl3. The increase in [Ca2+]i in both the LCC and CA1, was significantly decreased by the intracellular Ca2+ modulators thapsigargin and xestospongin C. The K+ channel activator isoflurane and Cl‐ channel blocker NPPB significantly decreased [Ca2+]i in the LCC. This study demonstrated that, between cells located in the LCC and in the CA1, the characteristics of brain edema induced by hypotonic stress are different. This can be ascribed to the different contribution of volume sensitive G‐protein coupled receptor and stretch sensitive Ca2+ channels.
Calcium dependence of spontaneous neurotransmitter release
Authors: Williams Courtney L. , Smith Stephen M. 
Published: July 2017

AbstractSpontaneous release of neurotransmitters is regulated by extracellular [Ca2+] and intracellular [Ca2+]. Curiously, some of the mechanisms of Ca2+signaling at central synapses are different at excitatory and inhibitory synapses. While the stochastic activity of voltage‐activated Ca2+channels triggers a majority of spontaneous release at inhibitory synapses, this is not the case at excitatory nerve terminals. Ca2+release from intracellular stores regulates spontaneous release at excitatory and inhibitory terminals, as do agonists of the Ca2+‐sensing receptor. Molecular machinery triggering spontaneous vesicle fusion may differ from that underlying evoked release and may be one of the sources of heterogeneity in release mechanisms.
A seasonal prediction for the wet–cold spells leading to winter crop damage in northwestern Taiwan with a combined empirical–dynamical approach
Authors: Promchote Parichart , Wang S.‐Y. Simon , Shen Yuan , Johnson Paul G. , Yao Ming‐Hwi 
Published: July 2017

ABSTRACTWinter crop losses from extreme weather in Taiwan have increased in the recent decade, with those losses associated with pronounced wet‐and‐cold events (temperature < 10 °C and precipitation >5 mm day−1). The regional and global patterns of atmospheric circulation and the sea surface temperature (SST) related to the extreme cold that damages fruits, vegetables, and paddy rice in northwest Taiwan were investigated. Cool SST anomalies in the western North Pacific (WNP) and warm SST in the central‐eastern Pacific associated with the Pacific meridional mode (PMM) shared a significant role in the occurrence of wet‐and‐cold events in northwest Taiwan. The interactions of the WNP/PMM with the North Pacific Oscillation (NPO) and the Central Pacific type of El Niño led to a pronounced lead–lag relationship with the occurrence of wet‐and‐cold events. An empirical model was subsequently developed to predict the wet‐and‐cold event frequency using observed values of WNP, Niño‐3.4, and Arctic Oscillation from year‐1 and predicted indices of WNP and PMM derived from the Climate Forecast System Version 2 (CFSv2) outputs. The predictive skill of this hybrid empirical–dynamical model was statistically significant throughout the 6 months leading up to the occurrence of wet‐and‐cold events.
Dynamic effects in the didehydro‐Diels‐Alder (DDDA) reaction of enyne‐ketoenes: 50% stepwise bond formation in spite of concerted transition state
Authors: Rana Anup , Paul Indrajit , Schmittel Michael 
Published: July 2017

AbstractThe C2─C6/Diels‐Alder cyclization of enyne‐ketoene 1 was studied by experiments, theoretical calculations, and dynamic trajectory computations. The failure to trap possible intermediate(s), indicates a concerted reaction mechanism. A detailed search for stationary points revealed a concerted mechanism and surprisingly a diradical intermediate with no direct connection to the enyne‐ketoene 1. To probe the accessibility of this intermediate quasiclassical trajectories were initiated from the concerted transition state structure. Notably, 36% of the trajectories reach the product zone directly and 5% arrive at the product via the intermediate diradical. Additionally, 31% of the trajectories go to the intermediate zone and stay there within the simulation time limit.
Ductal adenocarcinoma of the prostate: Clinical and biological profiles
Authors: Vinceneux Armelle , Bruyère Franck , Haillot Olivier , Charles Thomas , de la Taille Alexandre , Salomon Laurent , Allory Yves , Ouzaid Idir , Choudat Laurence , Rouprêt Morgan , Comperat Eva , Houede Nadine , Beauval Jean‐Baptiste , Vourc'h Patrick , Fromont Gaëlle 
Published: July 2017

BackgroundDuctal adenocarcinoma (DAC) is a rare and aggressive subtype of prostate cancer (PCa). In the present study, we analyzed the clinical and biological characteristics of DAC, in comparison with high grade conventional acinar PCa.MethodsSamples and data were retrospectively collected from seven institutions and centrally reviewed. Immunohistochemistry was performed on tissue microarrays to assess the expression of candidate proteins, based on the molecular classification of PCa, including ERG, PTEN, and SPINK1. SPOP mutations were investigated from tumor DNA by Sanger sequencing. Relationships with outcome were analyzed using log‐rank analysis and multivariable Cox regression.ResultsAmong 56 reviewed prostatectomy specimens, 45 cases of DAC were finally confirmed. The pathological stage was pT3 in more than 66% of cases. ERG was expressed in 42% of DAC, SPINK1 in 9% (all ERG‐negative), and two cases (ERG‐negative) harbored a SPOP mutation. Compared to high grade conventional PCa matched for the pathological stage, cell proliferation was higher (P = 0.04) in DAC, and complete PTEN loss more frequent (P = 0.023). In multivariate analysis, SPINK1 overexpression (P = 0.017) and loss of PSA immunostaining (P = 0.02) were significantly associated with biochemical recurrence.Conclusionthese results suggest that, despite biological differences that highlighted DAC aggressiveness, the molecular classification recently proposed in conventional PCa could also be applied in DAC.