bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Y44 residue point mutation in CXCL14 reveals the significance of CXCL14 protein stability in anti-tumor activity
Authors: Kamal Shah , Ullah Najeeb , Kong Xianglin , Amanullah Amanullah , Dai Ao , Kamal Mohammad Amjad , Deng Cheng , Shanshan Lai 
Published: September 2024

Abstract

Objectives
CXCL14, a member of the CXC chemokine family, plays a significant role in tumor development, progression, and metastasis, making it a potential target for cancer diagnosis and treatment. This study aims to investigate the impact of a point mutation in the (41VSRYR45) motif of CXCL14 on its anti-tumor activity.

Methods
Phylogenetic analysis, tertiary structure prediction, homology modeling, and cell culture experiments were employed to assess the effects of CXCL14 mutations on protein stability and anti-tumor activity. Phylogenetic analysis identified conserved regions critical for function, while computational tools predicted structural changes due to mutations. Homology modeling provided structural insights, and cell culture experiments involved transfecting HeLa cells with wild-type or mutant CXCL14 plasmids, assessing stability and anti-tumor effects. Additionally, in vivo, xenograft experiments using nude mice were conducted to evaluate the anti-tumor efficacy of CXCL14 variants.

Results
The conserved Y44 site within the (41VSRYR45) motif was identified as a key ubiquitination site. Mutations Y44V and Y44F enhanced CXCL14 stability, reducing HeLa cell proliferation and migration while increasing apoptosis. In vivo, tumor xenograft experiments confirmed the stronger inhibitory effect of these CXCL14 variants on tumor growth compared to the wild-type protein.

Conclusion
The Y44 site in CXCL14 is crucial for its stability and anti-tumor activity. Mutations Y44V and Y44F enhance CXCL14 stability and anti-tumor effects, suggesting that targeting this site could be a promising therapeutic strategy for cervical cancer.
Evaluation of HER2 immunohistochemistry-positive and immunohistochemistry-negative FISH amplification breast cancers using next-generation sequencing
Authors: Baba Shogo , Koyanagi Ai , Kakumoto Akinari , Kuroda Hajime , Harada Oi , Suzuki Megumi , Kato Yasutaka , Nishihara Hiroshi , Kawami Hiroyuki 
Published: September 2024

Abstract

Objectives
In this study, we aimed to assess the concordance between HER2 positivity, defined as scores of 2+ or 3+ by immunohistochemistry (IHC), and HER2 gene amplification results obtained by fluorescence in situ hybridization (FISH) and gene panel testing (GPT).

Methods
A total of 45 breast cancer patients with sufficient biopsy, breast-conserving surgery, or mastectomy specimens collected between 2018 and 2024 at Hokuto Hospital were included in the study. HER2 status was defined as HER2+ in cases with scores of 3+ on IHC staining, HER2-FISHamp in cases with scores of 2+ on IHC staining plus FISH amplification. FISH amplification was defined as a ratio of HER2 to the amplification of the chromosome 17 centromere ≥2.0. Next-generation sequencing (NGS) for these genes was conducted using MiSeq. The criterion for ERBB2 amplification requires gene amplification of more than 2 standard deviations (SD) above the average of the panel genes and CN ≥4.

Results
HER2+ correlated with the presence of NGS amplification of ERBB2 (p<0.001). NGS amplification was significantly more detectable in HER2+ than in HER2-FISHamp. HER2-FISHamp correlated with larger tumor size (p=0.009) and the presence of lymph node metastases (p<0.001). No correlations were observed between the HER2 status and other parameters such as age, histological grade, stage histological type, recurrence, treatment response evaluation, and breast cancer mortality.

Conclusions
To achieve a comprehensive diagnosis, it is crucial to integrate GPT findings with other diagnostic approaches and to have a though understanding of the characteristics and limitations of each method.
Crafting crossroads in Zagori (north-west Greece): Ottoman-era archaeology through a workshop in vernacular architecture
Authors: Moudopoulos-Athanasiou Faidon , Sklavounos Ionas , Koutropoulos Grigoris 
Published: September 2024

AbstractReconstruction of a nineteenth-century cobbled pathway in the village of Aristi provides valuable insights into the material culture and settlement archaeology of Ottoman-era Greece. The authors argue that such small-scale pairing of restoration and archaeological practices in ‘traditional’ settlements could enhance our understanding of Ottoman archaeology without undermining the lived experience of such places.
Finansowanie szkolnictwa wyższego w Stanach Zjednoczonych
Authors: Grabińska Barbara 
Published: September 2024

The American higher education system has been recognised as the best in the world, a fact borne out by international academic university rankings. The US has one of the most market-oriented higher education systems in the world and many countries look at the country's approach when undertaking to reform the funding of their own systems. The objective of the article is to present the characteristics of the US higher education system, emphasising its structure of financing and funding mechanisms.
“NEM PARECE CAXIAS!” DESLOCAMENTOS DOS SENTIDOS DE PERIFERIA EM COLETIVOS AUDIOVISUAIS NA BAIXADA FLUMINENSE DO RIO DE JANEIRO
Authors: Souza Adriana , Takeiti Akemi Beatriz 
Published: September 2024

Este artigo debate a produção de uma representação única de “periferia” e investiga o impacto da prática coletiva artística na produção de subjetividades em sujeitos que atuam em cineclubes na Baixada Fluminense do Rio de Janeiro. Trata-se de recorte de pesquisa de mestrado da primeira autora sobre produção de subjetividades em coletivos culturais a partir do campo da Psicossociologia de Comunidades. A pesquisa, de abordagem qualitativa e caráter cartográfico, selecionou coletivos artísticos que atuam como cineclubes, em especial, o Mate com Angu e Buraco do Getúlio, ambos localizados na Baixada Fluminense do Rio de Janeiro. A partir da realização de entrevistas biográficas, foram empreendidas análises de conteúdo (Bardin, 2011), nas quais foram observados deslocamentos semânticos dos sentidos de periferia presentes nos discursos dos interlocutores. As diferenças na formação histórica nos municípios da Baixada Fluminense geraram cenários múltiplos, desde territórios extremamente periferizados, passando por elites econômicas locais até vastas reservas naturais. Apesar desta diversidade, do ponto de vista da representação midiática, vemos a preponderância de narrativas operadores de estigmatização sobre este território e seus sujeitos. Nesta pesquisa, entretanto, os interlocutores parecem ilustrar os deslocamentos semânticos dos sentidos de periferia a partir da experiência coletiva em seu território, vendo-se enquanto sujeitos ao centro de uma produção simbólica e não à margem dela.
Rozwój stosunków gospodarczych Unii Europejskiej z krajami Azji a współpraca na rzecz zrównoważonego rozwoju
Authors: Drzymała Agnieszka 
Published: September 2024

Podręcznik jest prezentacją powiązań gospodarczych i środowiskowych Unii Europejskiej z krajami azjatyckimi. Koncentruje się na wskazaniu współpracy politycznej i ekonomicznej (na przykładzie handlu i bezpośrednich inwestycji zagranicznych), a także kwestii dotyczących ochrony środowiska (wybrane aspekty zrównoważonego rozwoju).
Celem publikacji jest przybliżenie Czytelnikowi wiedzy na temat podejmowanych działań proekologicznych w krajach ASEM poprzez pryzmat wzajemnych stosunków politycznych i ekonomicznych.
Książka przeznaczona jest głównie dla studentów wyższych uczelni kierunków ekonomicznych i ekologicznych. Jest to również podręcznik wspomagający proces dydaktyczny w ramach interdyscyplinarnego programu kształcenia w zakresie ekonomii ochrony środowiska.

Publikacja została przygotowana w ramach projektu „Utworzenie nowych interdyscyplinarnych programów kształcenia w zakresie ekonomii ochrony środowiska (w języku polskim i angielskim) w Uniwersytecie Łódzkim” współfinansowanego ze środków funduszy norweskich i krajowych. (Nr umowy FSS/2014/HEI/W/0124/U/0014)
Analisis perbandingan pendidikan multikultural Indonesia dengan Negara Amerika, Kanada dan Inggris
Authors: Ervista Eries Avril Aura , Fridayanti Alyah , Fachira Niken 
Published: September 2024

ABSTRAK
Pendidikan multikultural di negara maju menginginkan pendidikan yang menjunjung tinggi harkat dan martabat manusia dengan cara menghargai dan memahami perbedaan-perbedaan yang dimiliki masing-masing individu. Perbandingan pendidikan multikultural Pertama dari masing-masing negara maju ini memiliki persamaan dalam pendidikan multikultural yaitu ingin membratas adanya tindakantindakan diskriminasi dan ingin menegakkan hak-hak asasi manusia secara adil, Kedua Perbedaan pendidikan multikultural di Indonesia memberikan peran kepada Kepala Sekolah dalam memantau keberagaman yang dimiliki masing-masing siswa, di Amerika Serikat menerapkan pendidikan multikultural dengan cara memberikan edukasi kepada siswa mengenai keberagaman, di Inggris menerapkan pendidikan multikultural dengan cara sistem penerapan Bahasa, Bahasa inggris bukan menjadi Bahasa satu-satunya. Di Kanada menerapkan pendidikan multikultural dengan cara menerapkan model pendidikan yang merekonstruksi dari keanekaanragaraman budaya.
Kata kunci : multikultural, negara maju, pendidikan
Implementasi program Kampus Mengajar dalam pembentukan karakter tanggung jawab mahasiswa Fakultas Pendidikan Ilmu Pengetahuan Sosial Universitas Pendidikan Indonesia
Authors: Andriani Dewi , Abdulkarim Aim , Insani Nisrina Nurul 
Published: September 2024

ABSTRAK
Saat ini masih banyak permasalahan di Indonesia yang berkaitan dengan krisis karakter seperti tingginya tingkat korupsi di Indonesia, pelanggaran hukum, krisis identitas bangsa, dan menurunnya moralitas. Hal tersebut terjadi salah satunya akibat rendahnya nilai-nilai tanggung jawab. Saat ini sistem pembelajaran di perguruan tinggi hanya berorientasi pada hard-skill, sedangkan yang berorientasi pada karakter belum banyak diterapkan. Maka dari itu Nadiem Anwar Makarim selaku MENDIKBUD RI telah membuat terobosan baru yakni program kampus mengajar. Mahasiswa yang mengikuti program ini diarahkan menjadi seseorang yang taat aturan dan memiliki komitmen terhadap tugas-tugas yang mereka emban selama pelaksanaan berlangsung. Pendekatan deskriptif dilaksanakan terhadap Mahasiswa FPIPS UPI sebagai salah satu fakultas yang mahasiswanya banyak berpartisipasi melaksanakan kampus mengajar. Hasil yang diperoleh menunjukan bahwa kampus mengajar memberikan dampak kepada mahasiswa khususnya dalam pembentukan karakter tanggung jawab diantaranya : (1) kemampuan menyelesaikan tugas sesuai instruksi, (2) mengatur waktu dengan baik, (3) mampu membina kedisiplinan atau ketertiban, (4) mampu berkomunikasi dengan baik, (5) mampu bekerja sama dengan baik, (6) mampu menerima konsekuensi dari pilihan yang diambil saat melakukan kesalahan, (7) mampu menghindari kecurangan. Adapun hambatan dalam pelaksanaan program ini diantaranya : (1) keadaan atau karakter mahasiswa yang berbeda-beda, (2) program studi yang belum sepenuhnya memahami kebijakan yang ada dalam program kampus mengajar. (3) kurangnya partisipasi dari beberapa pihak sekolah dan masyarakat, (4) kurangnya mendapat apresiasi dari pihak perguruan tinggi. Namun beberapa pihak mampu menyelesaikan hambatan tersebut dengan berbagai upaya dan dukungan yang ada.
Kata kunci : kampus mengajar, karakter mahasiswa, sikap tanggung jawab
The Powers of The Soul in Late Franciscan Thought: The Case of Peter of Trabibus
Authors: Silva José Filipe , Vaura Tuomas 
Published: September 2024

In the late medieval period, the issue of the composed nature of human beings and its relation to medieval faculty psychology became central. There is ample scholarship on this topic, focusing primarily on authors such as the Dominicans Albert the Great and Thomas Aquinas, and the Franciscans Alexander of Hales, Hugh of St. Cher, John of La Rochelle, and Peter John Olivi. In this paper, we want to examine the view of one of Olivi’s disciples, the Franciscan theologian Peter of Trabibus (fl. 1290s), on the unity of the human soul and the nature of its powers, especially the powers of the intellect and the will. We are particularly interested in his application of the principle of plurality of substantial forms to explain the nature of the relation between the powers of the soul and its essence.
Nicotinylation is a histone mark generated from the nicotinic acid metabolite nicotinyl-CoA
Authors: Zhang Hong-Quan , Jiang Yuhan , Li Yixiao , Zhang Lei , Yan Haoyi , Qin Ziheng , Li Qianchen , Peng Yidi , Liu Jia , Weng Yejing , Cheng Zhongyi , Lv Danyu , Zhang Jing , Tang Yan , Zhan Jun 
Published: September 2024

Abstract

Protein post-translational modifications (PTMs) are crucial for cancer growth and metastasis. Vitamin B3, a key precursor of NAD + and NADP+, however its epigenetic functions in physiology and disease remain unclear. Herein we report a nicotinic acid (NA), a component of vitamin B3, induces a histone PTM, lysine nicotinylation (Knic), and demonstrate 17 Knic site across core histones in cells. Tandem mass spectrometry and stable isotope tracing revealed that NA-derived nicotinyl-CoA, catalysed by ACSS2, enhances histone Knic in vivo and in vitro. Analysis of chromatin accessibility revealed that histone Knic downregulates chromatin accessibility and therefore inhibits gene expression, for instance, restrain the binding of transcription factor HOXB9 to the promoter of oncogene PPFIA1. PPFIA1 level is correlated with malignancy and poor prognosis of hepatocellular carcinoma. These findings suggest that vitamin B3 supplementation may affects the chromatin accessibility depending on its composition. Collectively, we propose that NA induces histone Knic, a histone mark controlling gene expression.
Antibody responses against influenza A decline with successive years of annual influenza vaccination: results from an Australian Healthcare Worker cohort
Authors: Sullivan Sheena , Khvorov Arseniy , Carolan Louise , Dowson Leslie , Hadiprodjo Jessica , Sánchez-Ovando Stephany , Liu Yi , Leung Vivian , Hodgson David , Blyth Christopher , Macnish Marion , Cheng Allen , Hagenauer Michelle , Clark Julia , Dougherty Sonia , Macartney Kristine , Koirala Archana , Khatami Ameneh , Jadhav Ajay , Marshall Helen , Riley Kathryn , Wark Peter , Delahunty Catherine , Subbarao Kanta , Kucharski Adam , Fox Annette 
Published: September 2024

Abstract

Influenza vaccine effectiveness and immunogenicity can be compromised with repeated vaccination. We assessed immunological markers in a cohort of healthcare workers (HCW) from six public hospitals around Australia during 2020–2021. Sera were collected pre-vaccination and ~ 14 and ~ 180 days post-vaccination and assessed in haemagglutination inhibition assay against egg-grown vaccine and equivalent cell-grown viruses. Responses to vaccination were compared by the number of prior vaccinations. Baseline sera were available for 595 HCW in 2020 and 1031 in 2021. 5% had not been vaccinated during five years prior to enrolment and 55% had been vaccinated every year. Post-vaccination titres for all vaccine antigens were lowest among HCW vaccinated in all 5-prior years and highest among HCW with 0 or 1 prior vaccinations, even after adjustment. This was observed for both influenza A subtypes and was dependent on pre-vaccination titre. Expanded cohorts are needed to better understand how this translates to vaccine effectiveness.
Understanding the Impact of Literacy on Knowledge Gap and Behaviour Pattern among Diabetic Patients: A Cross-Sectional Study
Authors: Kaur Sandeep , Gupta G.D. 
Published: September 2024

Abstract

Background
Diabetes mellitus is a growing health concern affecting more than 6.2% of the global population.This cross-sectional study aimed to investigate the association of knowledge, attitude, and practice (KAP) of patients diagnosed with type II diabetes mellitus (DM II) with demographic and clinical variables in Punjab, India, focusing on education to understanding knowledge gap and behavioral pattern of patients influencing prevention and control of disease.
Methods
The study was conducted from March 2022 to September 2023 at three outpatient clinics in Punjab.Data were collected through a modified questionnaire, covering demographic profiles and KAP variablesusing non-probability purposive sampling of 500 respondents with SPSS software.
Results
The study analyzed 500 patients (197 females, 303 males) aged 31–90 years. Key findings revealed that knowledge scores were higher in males (33.7%), married individuals (32.3%), degree holders (60.0%), and insured patients (40.9%). Attitude scores were better in males (50.2%), degree holders (75.3%), and insured patients (57.9%). Practice scores were higher among females (25.9%), unmarried (43.3%), and insured patients (23.2%). Significant predictors of high KAP scores included education, patient history, blood sugar levels, clinic visits, marital status, occupation, health insurance, and physical activity. Education status had a substantial impact on all KAP scores.
Conclusion
This study highlights disparities in knowledge, attitudes, and practices related to DM II management among patients in North India. Addressing these disparities through targeted interventions, community-based programs, and improved healthcare accessibility is crucial for promoting positive outcomes in diabetes mellitus.
RORα inhibits proliferation and chemoresistance through AKR1A1-induced glucose and lipid reprogramming in gastric cancer
Authors: Wang Xiaoshan , Chen Mengding , Wu Yuwei , Chen Feixu , Shen Tong , Wang Zhengguang 
Published: September 2024

Abstract

Background Abnormal glycolysis and lipid synthesis play important roles in the occurrence and development of gastric cancer (GC).Moreover, dysregulation of circadian genes was associated with metabolic reprogramming in the tumor microenvironment. This study aimed to decipher the role of retinoic acid-related orphan receptor alpha (RORα) in glucose and lipid reprogramming of GC.
Methods The effects on GC growth in vitro and in vivo were studied using gain- and loss-of-function experiments. Glycolysis and lipid metabolism of GC cells were detected using seahorse assay and test kit. Moreover, regulatory mechanisms underlying RORα deletion were explored using half-life, Co-Immunoprecipitation (Co-IP), Chromatin immunoprecipitation (Chip), luciferase reporter and immunofluorescence co-localization assays in GC cells. In addition, the relationship of RORα with E47 and AKR1A1 was analyzed using clinicopathological retrospective analysis in two cohorts.
Results RORα deletion promoted proliferation and 5-FU chemoresistance by enhancing glycolytic activity and lipid synthesis. In contrast, SR1078, a RORα activator, reversed these phenomena and had a synergistic inhibitory effect on cell proliferation through combination with 2-deoxygulose glucose or atorvastatin. Mechanistically, RORα deletion promoted aldo-keto reductase family 1 member A1 (AKR1A1)-induced glycolysis and lipid metabolism to enhance cell proliferation and 5-FU chemoresistance. In detail, the protein stability of the AKR1A1 transcription factor E47 is modulated by β-catenin through binding to form a transcription heterodimer. RORα deletion enhanced wnt3a and β-catenin expression and indirectly inhibited E47 protein stability, leading to attenuated AKR1A1 transcriptional activity. Moreover, AKR1A1 expression was associated with clinicopathological parameters, including age, tumor-node-metastasis stage and tumor grade; AKR1A1 was found to be an independent predictor of GC prognosis and was negatively correlated with RORα expression. Moreover, the disease-free survival time of GC patients with RORα-high/E47-low/AKR1A1-low expression patterns was optimal. Furthermore, RORα, E47 or AKR1A1 expression alone or in combination with each other was associated with response status, the standard uptake value and lipid synthesis.
Conclusions These findings revealed a novel mechanism by which RORα regulates glucose and lipid reprogramming and might be a promising target for GC treatment.
Rare Synovial Sarcoma in the Oral Pharynx: A Case Study and Literature Review
Authors: Qi YanGe , Li Rong , Hu LiNa , Yan Xia , Bai XueQi , Han JiaLi , Song JianBo 
Published: September 2024

Abstract

Background: Synovial sarcoma (SS) is a rare cancer that is less common in the head and neck area. Because of its low incidence in this anatomical area, early diagnosis is difficult, and optimal treatment strategies remain uncertain. Here, we present a case of SS in the oropharynx.
Case presentation: The patient, a 70-year-old male, was admitted to the hospital with intermittent pain in the left gingiva, head, and face, and experienced dysphagia. biopsy proved to be SS. The patient did not agree to the surgery because of the extent of the resection. Thus, combined treatment based on radiotherapy and chemotherapy was performed. Post-treatment evaluations revealed significant reductions in the size of the oral pharynx lesion and lymph nodes, achieving a therapeutic response assessed as partial remission (PR).
Conclusions: The intricate anatomical landscape of the head and neck region poses significant challenges for the surgical management of SS. Combined treatment based on radiotherapy and chemotherapy offers avenues to achieve tumor regression for synovial sarcoma where surgery is not available.
Application of high-frequency ventilation combined with closed-loop automatic oxygen control therapy in neonatal respiratory distress syndrome
Authors: Huang Shan-biao , Lin Jia-Jia , Lin Hong , Fu Zi-zhou , Lin Yun-feng 
Published: September 2024

Abstract

Objective In the realm of retrospective research, the researcher embarked on a comprehensive inquiry with the primary aim of assessing the effectiveness and safety of high-frequency oscillatory ventilation (HFOV) in conjunction with closed-loop oxygen control for the management of severe neonatal respiratory distress syndrome (NRDS) in the pediatric population. The study population comprised 34 premature infants who had received a diagnosis of NRDS and were under the care of the Neonatal Intensive Care Unit (NICU) at Fujian Children's Hospital during the period spanning from April 2023 to January 2024. To facilitate a rigorous investigation, these participants were systematically stratified into two distinct cohorts, each adhering to specific treatment protocols.Method 17 premature infants who only received high-frequency ventilation as the control group and 17 premature infants who received high-frequency ventilation + closed-loop automatic oxygen control as the study group. The therapeutic effects and complications of the two groups were compared.Results The 2-hour “oxygen saturation of blood” and 4-hour “oxygen saturation of blood” in the control group were lower than those in the experimental group ( P < 0.05 ). The "PO2" of the control group after 1 day of treatment was higher than that of the experimental group ( P < 0.05 ).There was no statistically significant difference in the occurrence of VAP, pulmonary air leakage, pulmonary hemorrhage, grade III-IV intracranial hemorrhage, ROP, and BPD between the two groups ( P > 0.05 ). It is noteworthy that the number of hypoxic interventions in the control group was significantly higher than that in the experimental group, with a p-value less than 0.05.Conclusion The use of high-frequency ventilation + closed-loop automatic oxygen control in Premature infants with NRDS has a positive effect on reducing the occurrence of hypoxemia during treatment, which can significantly reduce the number of interventions when hypoxemia occurs. However, there is no significant difference in the occurrence of common long-term complications such as bronchopulmonary dysplasia in premature infants.