bluuebunny/crossref_metadata_2025_split huggingface dataset:
SOX12: a novel potential target for acute myeloid leukaemia
Authors: Wan Haixia , Cai Jiayi , Chen Fangyuan , Zhu Jianyi , Zhong Jihua , Zhong Hua 
Published: November 2016

SummaryThe role of SRY‐related high‐mobility‐group box (SOX) 12 in leukaemia progression and haematopoiesis remains elusive. This study aimed to examine the expression and function of SOX12 in acute myeloid leukaemia (AML) using human myeloid leukaemia samples and the acute myeloid cell line THP1. Mononuclear cells were isolated from the bone marrow of AML patients and healthy donors. SOX12 expression in haematopoietic cells was evaluated by reverse transcription polymerase chain reaction (RT‐PCR). SOX12 short hairpin RNAs (shRNAs) were transduced into THP1 cells, and gene knockdown was confirmed by quantitative RT‐PCR and Western blot analysis. SOX12 was preferentially expressed in CD34+ cells in AML patients. The THP1 cells transduced with SOX12 shRNAs exhibited significantly reduced SOX12 expression and cell proliferation. SOX12 knockdown had no effect on apoptosis, but it induced cell cycle arrest at G1 phase and reduced the number of colonies. The transduced THP1 and primary AML cells were reconstituted in non‐obese diabetic‐severe combined immunodeficient (NOD/SCID) mice, and their numbers were significantly reduced 6–12 weeks after transplantation. The mRNA and protein levels of β‐catenin were significantly diminished following SOX12 knockdown, accompanied by a decrease in TCF/Wnt activity. SOX12 may be involved in leukaemia progression by regulating the expression of β‐catenin and then interfering with TCF/Wnt pathway, which may be a target for AML.
A phase IIb trial of vorinostat in combination with lenalidomide and dexamethasone in patients with multiple myeloma refractory to previous lenalidomide‐containing regimens
Authors: Sanchez Larysa , Vesole David H. , Richter Joshua R. , Biran Noa , Bilotti Elizabeth , McBride Laura , Anand Palka , Ivanovski Kristin , Siegel David S. 
Published: November 2016

SummaryClinical trials of vorinostat, a Class I/II histone deacetylase inhibitor, in combination with proteasome inhibitors and immunomodulatory agents have shown activity in relapsed/refractory multiple myeloma. This phase IIb, open‐label, single‐institution study evaluated the efficacy of vorinostat in combination with lenalidomide and dexamethasone in lenalidomide‐refractory patients. Patients were considered lenalidomide‐refractory if they had no clinical response (<minimal response) on a previous lenalidomide‐containing regimen (lenalidomide non‐responsive) or if they had progressive disease on or within 60 days of discontinuing a previous lenalidomide‐containing regimen (lenalidomide relapsed/refractory). Patients received oral vorinostat 400 mg days 1–7 and 15–21, lenalidomide 25 mg days 1–21, and dexamethasone 40 mg days 1, 8, 15 and 22 in 28‐day cycles. Twenty‐five patients were enrolled, median age was 65 years and patients had received a median of 5 prior regimens. The overall response rate was 24% (6 partial responses) and clinical benefit rate (≥stable disease) was 80%. Median time to a partial response was 1·9 months and median duration of response was 3·3 months. Median progression‐free survival was 5·3 months. Most common grade 3/4 adverse events were neutropenia (48%), thrombocytopenia (32%), anaemia (20%) and gastrointestinal toxicities (16%). In this heavily pre‐treated population, vorinostat in combination with lenalidomide and dexamethasone was active in lenalidomide‐refractory patients.
Visualization of Biomolecular Structures: State of the Art Revisited
Authors: Kozlíková B. , Krone M. , Falk M. , Lindow N. , Baaden M. , Baum D. , Viola I. , Parulek J. , Hege H.‐C. 
Published: November 2016

AbstractStructural properties of molecules are of primary concern in many fields. This report provides a comprehensive overview on techniques that have been developed in the fields of molecular graphics and visualization with a focus on applications in structural biology. The field heavily relies on computerized geometric and visual representations of three‐dimensional, complex, large and time‐varying molecular structures. The report presents a taxonomy that demonstrates which areas of molecular visualization have already been extensively investigated and where the field is currently heading. It discusses visualizations for molecular structures, strategies for efficient display regarding image quality and frame rate, covers different aspects of level of detail and reviews visualizations illustrating the dynamic aspects of molecular simulation data. The survey concludes with an outlook on promising and important research topics to foster further success in the development of tools that help to reveal molecular secrets.
Compliance with a COPD bundle of care in an Australian emergency department: A cohort study
Authors: Gerber Alexis , Moynihan Catriona , Klim Sharon , Ritchie Peter , Kelly Anne‐Maree 
Published: November 2016

AbstractIntroductionBundles of care are gaining popularity for treating acute severe illness.ObjectiveTo describe compliance with bundle of care elements (individually and as a “bundle”) for patients treated for chronic obstructive pulmonary disease (COPD) exacerbations in the emergency department (ED).MethodsRetrospective observational study of patients presenting in the 2014 calendar year with an ED diagnosis of COPD. The primary outcomes of interest were compliance with key bundle of care elements (individually and as a “bundle”). Analysis is descriptive.Results381 patients were studied. Median age was 71 (IQR 64‐80), 60% were male and 77% arrived by ambulance. Median duration of symptoms was 3 days (IQR 2‐6 days). Compliance with the bundle elements was 90% for administration of controlled oxygen therapy (if oxygen given), 87% for administration of inhaled bronchodilators, 79% for administration of systemic corticosteroids, 75% of administration of antibiotics if evidence of infection, 77% for taking of a blood gas in non‐mild disease, 98% for taking of a chest X‐ray, and 74% for administration of NIV if pH <7.3. Compliance with all appropriate elements of the defined bundle of care was 49%. There was no difference in mean length of stay for admitted patients (P = .44), in‐hospital mortality (P = 1.00) or re‐admission within 30 days (P = .72) by bundle compliance.ConclusionCompliance with individual assessment and treatment recommendations was generally high; however, compliance with the overall recommended bundle was only 49%. This indicates that there is an opportunity to improve care in these patients.
Can Service Climate Detract from Employee Performance? The Role of Experience in Optimizing Satisfaction and Performance Outcomes
Authors: Gabler Colin B. , Rapp Adam , Richey Robert Glenn , Adams Frank G. 
Published: November 2016

ABSTRACTIn this research, we test the curvilinear relationships between service climate perceptions and two employee performance outcomes. Specifically, we propose that while service climate can be beneficial, high levels can actually be detrimental to customer satisfaction and sales performance. Additionally, we propose that a global assessment of employee experience that captures knowledge, skills, and abilities, or KSAs, moderates these curvilinear relationships by providing a means to balance outcome goals. We test our theory using data obtained from 312 employees in a service setting, which we pair with their managers’ assessments of their sales performance as well as satisfaction ratings from their customers. Our results reveal two things: (1) an inverted U‐shaped relationship between service climate and sales performance and (2) the level of experience moderates the relationship. These findings suggest that more experienced employees are better able to adjust behaviors to achieve high levels of performance than less experienced employees. Based on these results, we offer theoretical implications and applications for managerial practice.
Socio‐economic factors influencing the development of end‐stage renal disease in people with Type 1 diabetes – a longitudinal population study
Authors: Toppe C. , Möllsten A. , Schön S. , Dahlquist G. 
Published: November 2016

AbstractAimsThe development of end‐stage renal disease (ESRD) in Type 1 diabetes is multifactorial. Familial socio‐economic factors may influence adherence to and understanding of diabetes treatment, and also general health behaviour. We investigate how parental and personal education level and exposure to low economic status, indicated by the need for income support, influence the development of ERSD caused by Type 1 diabetes.MethodsParticipants were retrieved from the nationwide Swedish Childhood Diabetes Registry, which was linked to the Swedish Renal Registry, to find people with ESRD caused by Type 1 diabetes, and to Statistic Sweden to retrieve longitudinal socio‐economic data on participants and their parents. Data were analysed using Cox regression modelling.ResultsOf 9287 people with diabetes of duration longer than 14 years, 154 had developed ESRD due to diabetes. Median diabetes duration (range) for all participants was 24.2 years (14.0–36.7 years). Low maternal education (≤ 12 years) more than doubled the risk of developing ESRD, hazard ration (HR) = 2.9 [95% confidence interval (95% CI): 1.7‐4.8]. For people with a low personal level of education HR was 5.7 (3.4–9.5). In an adjusted model, the person's own education level had the highest impact on the risk of ESRD. If at least one of the parents had ever received income support the HR was 2.6 (1.9–3.6).ConclusionsSocio‐economic factors, both for the parents and the person with diabetes, have a strong influence on the development of ESRD in Type 1 diabetes. It is important for caregivers to give enough support to more vulnerable people and their families.
A comparative study of approaches to forecast the correct trading actions
Authors: Baía Luís , Torgo Luís 
Published: November 2016

AbstractThis paper addresses the problem of decision making in the context of financial markets, more specifically, the problem of forecasting the correct trading action for a certain future horizon. We study and compare two alternative ways of addressing these forecasting tasks: (a) using standard numeric prediction models to forecast the variation on the prices of the target asset and, on a second stage, transform these numeric predictions into a decision according to some predefined decision rules; and (b) use models that directly forecast the right decision thus ignoring the intermediate numeric forecasting task. The objective of our study is to determine if both strategies provide identical results or if there is any particular advantage worth being considered that may distinguish each alternative in the context of financial markets.
Shifts in Intergenerational Mobility of Indian Immigrant Entrepreneurs
Authors: Chavan Meena , Taksa Lucy 
Published: November 2016

AbstractThe objective of this article is to examine the shift in the intergenerational mobility of Indian immigrant entrepreneurs in Australia. Based on a qualitative methodology, this article reports on the differences in the entrepreneurial attitudes of push and pull and the aptitudes of social and human capital between pre 2000 and post 2000 immigrant entrepreneurs. The findings suggest that the post 2000 Indian migrant entrepreneurs in Australia are mostly pull motivated, have higher qualifications than the pre 2000 arrivals, speak better English, have professional educational qualifications relevant to their business, and operate predominantly in the service sector. They take fewer years to get into business and are less dependent on immigrant social capital resources than pre 2000 arrivals. The study proposes that, compared with social capital resources, human capital resource have a greater impact on entrepreneurial propensity in the case of second generation Indian migrant entrepreneurs in Australia.
Policy Implications

This research has implication for Australian immigration policy, labour laws and settlement services of migrants. It recommends successful implementation of policies and durable solutions for Indian immigrants in the labour market in Australia.

The Australian Government will be assisted in examining and identifying future options for the intake of temporary and permanent migrants that improve the income, wealth and living standards of Australian citizens, improve the budgets and balance sheets of Australian governments, minimize administration and compliance costs associated with immigration, and provide pathways both for Australian citizens to be altruistic towards foreigners, and for Australia's international responsibilities and obligations to foreign residents to be met. Improvements in the labour laws would promote the effective integration of Indian immigrants into society.

Further, Indians in the USA have contributed immensely to the entrepreneurial spirit due to the government support for migrant SMEs and the small business venture funds. The Australian government can replicate this policy, reduce restriction on employment opportunities and enhance entrepreneurship for all migrants.
A Comparison of Women and Men Undergoing Catheter Ablation for Sustained Monomorphic Ventricular Tachycardia
Authors: BALDINGER SAMUEL H. , KUMAR SAURABH , ROMERO JORGE , FUJII AKIRA , EPSTEIN LAURENCE M. , MICHAUD GREGORY F. , JOHN ROY , TEDROW USHA B. , STEVENSON WILLIAM G. 
Published: November 2016

Ventricular Tachycardia Ablation in Women Compared to MenIntroductionWomen are underrepresented in clinical studies on catheter ablation (CA) for ventricular tachycardia (VT). The role of gender as a predictor for VT recurrence after CA is unclear and complication rates have not been compared in a large cohort.Methods and ResultsWe analyzed data of consecutive patients undergoing CA for sustained monomorphic VT at our center between 2005 and 2015.A total of 948 patients underwent 1314 ablation procedures: 114 patients without structural heart disease (SHD) (48% female), 486 with coronary artery disease (CAD) (9% female), 301 with nonischemic cardiomyopathy (NICM) (22% female), and 46 with arrhythmogenic right ventricular cardiomyopathy (ARVC) (17% female). Women with CAD and NICM were younger than men at first ablation (63 years vs. 68 years, P = 0.05; resp. 53 years vs. 59 years, P = 0.026) with no other significant differences in baseline characteristics. Age, LVEF, NYHA‐class, and VT‐recurrence but not gender were independently associated with increased mortality in CAD and NICM. Mortality rates in patients with no SHD and ARVC are low in men (0%, 2.6%) and women (1.8%, 0%).ConclusionsAlthough heart disease tends to present later in women, our data do not suggest that women are referred later than men. Women with CAD or NICM and VT present for ablation at younger age with disease severity comparable to men. VT ablation in women can be accomplished with success‐ and complication rates comparable to male patients in both those with and without SHD.
A randomized two-way crossover bioequivalence study in healthy adult volunteers of paediatric zidovudine/lamivudine/nevirapine fast-disintegrating fixed-dose combination tablet
Authors: Joshi Anjali , Gbadero Daniel , Esseku Fredrick , Adesanya Olufikayo J , Adeyeye Moji C 
Published: November 2016

Abstract

Objective
The bioequivalence study was conducted to compare the developed paediatric fixed-dose combination (FDC) zidovudine/lamivudine/nevirapine (60/30/50 mg) tablet – the test formulation – with the combined mixture of single-entity innovator products (reference product).

Methods
A single-dose open-label randomized two-way crossover study was conducted in healthy adult African volunteers after an informed consent was obtained. The 24 volunteers, divided into two groups, were administered the products after an overnight fast on two treatment days with 14 days of washout period. Blood samples were collected for 96 h and analysed using a validated RP-HPLC-UV assay method. Pharmacokinetic (PK) parameters (non-compartmental model) were assessed with WinNonlin® software. Analysis of variance (ANOVA) and FDA bioequivalence statistical criterion of 90% CI or 80% to 125% range (set at P &lt; 0.05) of least square geometric means (LSGM) ratios of test: reference product for Cmax, AUC0–t, and AUC0–∞ were determined.

Results
ANOVA indicated that the period, sequence and formulation had no significant effect on the PK parameters (P &gt; 0.05). The 90% CIs for all the drugs were within the 80% to 125% range.

Conclusion
The developed FDC tablet is bioequivalent to the reference product.
Effect of soaking, fermentation with lactobacillus and enzyme treatment on nitrogen‐corrected apparent metabolizable energy values of wheat in cockerels and quails
Authors: Alahyari‐Shahrasb M. , Moravej H. , Kianfar R. , Kim W. K. 
Published: November 2016

SummaryAn experiment was carried out to determine nitrogen‐corrected apparent metabolizable energy (AMEn) values of processed wheat products with two levels of enzyme (0 and 0.5 g/kg) and five processing methods [control (C), water‐soaking for 12 h (SO12h), 24 h (SO24h) and 48 h (SO48h) and fermentation with lactobacillus (FL)] in two species of poultry (cockerel and Japanese quail). In this study, each processing method group had six replications with two cockerels or four quails per replication. The results showed that the calculated AMEn values of processed wheat by different methods were as follows: C‐12.61 and 12.60; SO12h‐12.75 and 12.73; SO24h‐12.80 and 12.79; SO48h‐12.97 and 12.96; and FL‐13.36 and 13.33 MJ/kg for cockerels and quails, respectively, indicating that fermentation improved AMEn values compared with the others. The mean AMEn of processed wheat with enzyme (13.12 MJ/kg) was significantly higher than that of processed wheat without enzyme (12.64 MJ/kg). However, there were no significant differences in the AMEn of processed wheat between cockerels and quails. The interaction between species and levels of enzyme in AMEn values was not significant. There was no significant difference (p > 0.05) in three‐way interactions among species, levels of enzyme and wheat processing methods for AMEn values. In conclusion, the results of the experiment suggest that the FL was more effective processing method of wheat to obtain high level of AMEn values than the others, and the combination of enzyme and processing methods can increase the AMEn values compared with processing methods alone.
Plane of nutrition affects growth rate, organ size and skeletal muscle satellite cell activity in newborn calves
Authors: MacGhee M. E. , Bradley J. S. , McCoski S. R. , Reeg A. M. , Ealy A. D. , Johnson S. E. 
Published: November 2016

SummaryPlane of nutrition effects on body, tissue and cellular growth in the neonatal calf are poorly understood. The hypothesis that a low plane of nutrition (LPN) would limit skeletal muscle size by reducing fibre growth and muscle progenitor cell activity was tested. At birth, calves were randomly assigned to either a LPN (20% CP, 20% fat; GE=1.9 Mcal/days) or a high plane of nutrition (HPN; 27% CP, 10% fat, GE = 3.8 Mcal/days) in a 2 × 3 factorial design to test the impact of diet on neonatal calf growth, organ weight and skeletal muscle morphometry with time. Groups of calves (n = 4 or 5) were euthanised at 2, 4 and 8 week of age and organ and empty carcass weights were recorded. Body composition was measured by DXA. Longissimus muscle (LM) fibre cross‐sectional area (CSA), fibre/mm2 and Pax7 were measured by immunohistology. Satellite cells were isolated at each time point and proliferation rates were measured by EdU incorporation. Calves fed a HPN had greater (p < 0.05) BW, ADG and hip height than those fed a LPN for 2, 4 or 8 weeks. HPN calves contained a greater (p < 0.05) percentage of fat tissue than LPN calves. Liver, spleen and thymus weights were less (p < 0.05) in LPN calves than HPN animals. Calves fed HPN had larger (p < 0.05) LM CSA at 8 weeks than LPN fed animals with no differences between the groups in numbers of satellite cells per fibre. Proliferation rates of satellite cells isolated from HPN fed calves were greater (p < 0.05) at 2 weeks than LPN fed animals, which exhibited greater (p < 0.05) proliferation rates at 4 weeks than HPN fed calves. We conclude a LPN diet reduces body growth and organ size and metabolically reprograms satellite cell activity.
Effect of particle size and partial replacement of alfalfa hay by soya bean hulls on nutrient intake, total tract digestibility and rumen degradability of diets by Holstein steers
Authors: Behgar M. , Valizadeh R. , Naserian A. A. , Ghasemi S. , Vahmani P. 
Published: November 2016

SummaryThis study examined the effect of alfalfa hay (AH) particle size and the replacement of soya bean hull (SH) for AH within the diet of restricted fed Holstein steers on dry matter intake (DMI), total tract digestion, ruminal digestion, ruminal pH and ammonia nitrogen content, and faecal pH. Four rumen‐cannulated Holstein steers averaging 353 ± 9.6 kg of BW were assigned to a 4 × 4 Latin square experiment with four periods and a 2 × 2 factorial arrangement of treatments. Factor A was AH particle size (fine vs. coarse) and factor B was diet SH content (0% vs. 10%; substituted for AH). Steers were fed at 1% of body weight of TMR containing 400 g/kg forage and 600 g/kg concentrate. Chopping of AH to fine particles decreased (p = 0.01) amount of dietary materials retained on the medium sieve (8 mm). The inclusion of SH significantly increased (p = 0.01) materials retained on the 1.18‐mm sieve and tended to decrease (p = 0.07) materials on 19‐mm sieves. The inclusion of SH increased (p = 0.01) ether extract (EE) intake and increased (p = 0.07) DMI. Inclusion of SH increased (p = 0.01) EE digestion and decreased (p < 0.01) faecal pH. Neither AH particle size nor SH inclusion in diets affected (p > 0.10) the in situ ruminal degradability coefficients of DM (‘a’, ‘b’, ‘c’ or ‘a+b’). No interaction of AH×SH was seen on nutrient intake, digestibility and in situ ruminal degradability of Holstein steers.
Zinc methionine and laminarin have growth‐enhancing properties in newly weaned pigs influencing both intestinal health and diarrhoea occurrence
Authors: Bouwhuis M. A. , Sweeney T. , Mukhopadhya A. , Thornton K. , McAlpine P. O. , O'Doherty J. V. 
Published: November 2016

SummaryThree experiments were conducted to investigate the interaction between zinc methionine (ZnM) and laminarin (LAM) on piglet growth performance and intestinal health post‐weaning. Experiment 1 was designed as 2 × 2 factorial with four treatments [n = 8, weaning age (WA) 24 days, live weight (LW) 7.15 kg]: (i) basal diet (BD); (ii) BD + 500 mg/kg ZnM; (iii) BD + 300 mg/kg LAM; and (iv) BD + 500 mg/kg ZnM + 300 mg/kg LAM. There was an interaction (p < 0.05) between LAM and ZnM. Pigs that were offered the LAM diet had a similar performance to the BD. However, when combining LAM with ZnM, pigs had reduced average daily gain (ADG), gain‐to‐feed ratio (G:F) and LW at slaughter at day 8 post‐weaning compared to the ZnM. Both LAM and ZnM improved the small intestinal morphology of the pigs at day 8 post‐weaning. Experiment 2 was designed as 2 × 2 factorial with four dietary treatments (n = 9, WA 24 days, LW 7.32 kg): (i) BD; (ii) BD + 500 mg/kg ZnM; (iii) BD + 175 mg/kg LAM; and (iv) BD + 500 mg/kg ZnM + 175 mg/kg LAM. The ADG and average daily feed intake were improved between day 0 and 31 PW when pigs were offered a LAM diet (p < 0.01). Faecal scores were reduced between day 0 and day 31 post‐weaning with ZnM (p < 0.001). Experiment 3 consisted of four dietary treatments (n = 10, WA 24 days, LW 7.32 kg): (i) BD; (ii) BD + 3300 mg/kg zinc oxide (ZnO); (iii) BD + 500 mg/kg ZnM; and (iv) BD + 175 mg/kg LAM. Pigs that were offered the ZnO diet had an increased ADG compared to the BD or ZnM diets (p < 0.01). Pigs that were offered the LAM diet had increased ADG compared to the ZnM diet (p < 0.05). Faecal scores were reduced between day 0 and day 31 PW with ZnM or ZnO supplementation (p < 0.001). In conclusion, the inclusion of 175 mg/kg LAM and ZnO improved ADG while both ZnO and ZnM reduced the faecal scores post‐weaning.