bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multidisciplinary approach to study the reproductive biology of wild prawns
Authors: Bolognini L. , Donato F. , Lucchetti A. , Olivotto I. , Truzzi C. , Randazzo B. , Antonucci M. , Illuminati S. , Grati F. 
Published: November 2017

AbstractThis work aims to provide deeper knowledge on reproductive biology of P. kerathurus in a multidisciplinary way. Upon 789 examined females, 285 were found inseminated. The logistic equation enabled to estimate the size at first maturity at 30.7 mm CL for female. The Gono-Somatic Index (GSI) showed a pronounced seasonality, ranged from 0.80 ± 0.34 to 11.24 ± 5.72. Histological analysis highlighted five stages of ovarian development. Gonadal fatty acids analysis performed with gas chromatograph evidenced a pronounced seasonal variation; total lipids varied from 1.7% dry weight (dw) in Winter, to 7.2% dw in Summer. For the first time, a chemometric approach (Principal Component Analysis) was applied to relate GSI with total lipid content and fatty acid composition of gonads. The first two components (PC1 and PC2) showed that seasonality explained about 84% of the variability of all data set. In particular, in the period February-May, lipids were characterized by high PUFAs content, that were probably utilized during embryogenesis as energy source and as constituent of the cell membranes. During the summer season, gonads accumulated saturated FAs, that will be used during embryogenesis and early larval stages, while in the cold season total lipids decreased drastically and the gonad reached a quiescent state.
Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells
Authors: Tabe Shirou , Hikiji Hisako , Ariyoshi Wataru , Hashidate-Yoshida Tomomi , Shindou Hideo , Shimizu Takao , Okinaga Toshinori , Seta Yuji , Tominaga Kazuhiro , Nishihara Tatsuji 
Published: November 2017

AbstractGlycerophospholipids have important structural and functional roles in cells and are the main components of cellular membranes. Glycerophospholipids are formed via the de novo pathway (Kennedy pathway) and are subsequently matured in the remodeling pathway (Lands’ cycle). Lands’ cycle consists of two steps: deacylation of phospholipids by phospholipases A2 and reacylation of lysophospholipids by lysophospholipid acyltransferases (LPLATs). LPLATs play key roles in the maturation and maintenance of the fatty acid composition of biomembranes, and cell differentiation. We examined whether LPLATs are involved in chondrogenic differentiation of ATDC5 cells, which can differentiate into chondrocytes. Lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards 18:1-, 18:2-, 20:4-, and 22:6-CoA increased in the late stage of chondrogenic differentiation, when mineralization occurred. LPCAT4 knockdown decreased mRNA and protein levels of chondrogenic markers as well as Alcian blue staining intensity and alkaline phosphatase activity in ATDC5 cells. These results suggest that LPCAT4 plays important roles during the transition of chondrocytes into hypertrophic chondrocytes and/or a mineralized phenotype.
Thermally nucleated magnetic reversal in CoFeB/MgO nanodots
Authors: Meo Andrea , Chureemart Phanwadee , Wang Shuxia , Chepulskyy Roman , Apalkov Dmytro , Chantrell Roy W. , Evans Richard F. L. 
Published: November 2017

AbstractPower consumption is the main limitation in the development of new high performance random access memory for portable electronic devices. Magnetic RAM (MRAM) with CoFeB/MgO based magnetic tunnel junctions (MTJs) is a promising candidate for reducing the power consumption given its non-volatile nature while achieving high performance. The dynamic properties and switching mechanisms of MTJs are critical to understanding device operation and to enable scaling of devices below 30 nm in diameter. Here we show that the magnetic reversal mechanism is incoherent and that the switching is thermally nucleated at device operating temperatures. Moreover, we find an intrinsic thermal switching field distribution arising on the sub-nanosecond time-scale even in the absence of size and anisotropy distributions or material defects. These features represent the characteristic signature of the dynamic properties in MTJs and give an intrinsic limit to reversal reliability in small magnetic nanodevices.
Automated VSS-based Burn Scar Assessment using Combined Texture and Color Features of Digital Images in Error-Correcting Output Coding
Authors: Pham Tuan D. , Karlsson Matilda , Andersson Caroline M. , Mirdell Robin , Sjoberg Folke 
Published: November 2017

AbstractAssessment of burn scars is an important study in both medical research and clinical settings because it can help determine response to burn treatment and plan optimal surgical procedures. Scar rating has been performed using both subjective observations and objective measuring devices. However, there is still a lack of consensus with respect to the accuracy, reproducibility, and feasibility of the current methods. Computerized scar assessment appears to have potential for meeting such requirements but has been rarely found in literature. In this paper an image analysis and pattern classification approach for automating burn scar rating based on the Vancouver Scar Scale (VSS) was developed. Using the image data of pediatric patients, a rating accuracy of 85% was obtained, while 92% and 98% were achieved for the tolerances of one VSS score and two VSS scores, respectively. The experimental results suggest that the proposed approach is very promising as a tool for clinical burn scar assessment that is reproducible and cost-effective.
Possible Tomonaga-Luttinger spin liquid state in the spin-1/2 inequilateral diamond-chain compound K3Cu3AlO2(SO4)4
Authors: Fujihala Masayoshi , Koorikawa Hiroko , Mitsuda Setsuo , Morita Katsuhiro , Tohyama Takami , Tomiyasu Keisuke , Koda Akihiro , Okabe Hirotaka , Itoh Shinichi , Yokoo Tetsuya , Ibuka Soshi , Tadokoro Makoto , Itoh Masaki , Sagayama Hajime , Kumai Reiji , Murakami Youichi 
Published: November 2017

AbstractK3Cu3AlO2(SO4)4 is a highly one-dimensional spin-1/2 inequilateral diamond-chain antiferromagnet. Spinon continuum and spin-singlet dimer excitations are observed in the inelastic neutron scattering spectra, which is in excellent agreement with a theoretical prediction: a dimer-monomer composite structure, where the dimer is caused by strong antiferromagnetic (AFM) coupling and the monomer forms an almost isolated quantum AFM chain controlling low-energy excitations. Moreover, muon spin rotation/relaxation spectroscopy shows no long-range ordering down to 90 mK, which is roughly three orders of magnitude lower than the exchange interaction of the quantum AFM chain. K3Cu3AlO2(SO4)4 is, thus, regarded as a compound that exhibits a Tomonaga-Luttinger spin liquid behavior at low temperatures close to the ground state.
Squalene epoxidase plays a critical role in determining pig meat quality by regulating adipogenesis, myogenesis, and ROS scavengers
Authors: Ha Jeongim , Kwon Seulgi , Hwang Jung Hye , Park Da Hye , Kim Tae Wan , Kang Deok Gyeong , Yu Go Eun , Park Hwa Chun , An Sang Mi , Kim Chul Wook 
Published: November 2017

AbstractIn mammals, Squalene epoxidase (SQLE) is an enzyme that converts squalene to 2,3-oxidosqualene, in the early stage of cholesterol generation. Here, we identified single nucleotide polymorphisms (SNPs) in the SQLE gene (c.2565 G > T) by RNA Sequencing from the liver tissue of Berkshire pigs. Furthermore, we found that homozygous GG pigs expressed more SQLE mRNA than GT heterozygous and TT homozygous pigs in longissimus dorsi tissue. Next, we showed that the SNP in the SQLE gene was associated with several meat quality traits including backfat thickness, carcass weight, meat colour (yellowness), fat composition, and water-holding capacity. Rates of myogenesis and adipogenesis induced in C2C12 cells and 3T3-L1 cells, respectively, were decreased by Sqle knockdown. Additionally, the expression of myogenic marker genes (Myog, Myod, and Myh4) and adipogenic marker genes (Pparg, Cebpa, and Adipoq) was substantially downregulated in cells transfected with Sqle siRNA. Moreover, mRNA expression levels of ROS scavengers, which affect meat quality by altering protein oxidation processes, were significantly downregulated by Sqle knockdown. Taken together, our results suggest the molecular mechanism by which SNPs in the SQLE gene can affect meat quality.
Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions
Authors: Wang B. , Liu W. , Xing D. , Li R. , Lv C. , Li Y. , Yan X. , Ke Y. , Xu Y. , Du Y. , Lin J. 
Published: November 2017

AbstractSubchondral bone has been identified as an attractive target for KOA. To determine whether a minimally invasive micro-scaffolds could be used to induce regeneration of knee subchondral bone lesions, and to examine the protective effect of subchondral bone regeneration on upper cartilage, a ready-to-use injectable treatment with nanohydroxyapatite-chitosan-gelatin micro-scaffolds (HaCGMs) is proposed. Human-infrapatellar-fat-pad-derived adipose stem cells (IPFP-ASCs) were used as a cellular model to examine the osteo-inductivity and biocompatibility of HaCGMs, which were feasibly obtained with potency for multi-potential differentiations. Furthermore, a subchondral bone lesion model was developed to mimic the necrotic region removing performed by surgeons before sequestrectomy. HaCGMs were injected into the model to induce regeneration of subchondral bone. HaCGMs exhibited desirable swelling ratios, porosity, stiffness, and bioactivity and allowed cellular infiltration. Eight weeks after treatment, assessment via X-ray imaging, micro-CT imaging, and histological analysis revealed that rabbits treated with HaCGMs had better subchondral bone regeneration than those not treated. Interestingly, rabbits in the HaCGM treatment group also exhibited improved reservation of upper cartilage compared to those in other groups, as shown by safranin O-fast green staining. Present study provides an in-depth demonstration of injectable HaCGM-based regenerative therapy, which may provide an attractive alternative strategy for treating KOA.
Rapid and Sensitive SERS Detection of Bisphenol A Using Self-assembled Graphitic Substrates
Authors: Lin Pei-Ying , Hsieh Chiung-Wen , Hsieh Shuchen 
Published: November 2017

AbstractWe have prepared and tested a new surface enhanced Raman scattering (SERS) substrate based on self-assembled graphitic sheets to detect bisphenol A (BPA) in plastic consumer goods. Transmission electron microscopy (TEM) and atomic-force microscopy (AFM) were used to characterize the structure of the graphitic sheets and showed a lattice spacing of 0.24 nm and layer height of 0.34 nm. These values were comparable to single monolayer graphene. The effective SERS detection limit of this method is 1 μM BPA, which is lower than the European Union specific migration limit for BPA of 0.6 mg/kg (2.6 μM). When used in salt solutions, graphitic sheets exhibited ultra-sensitivity toward BPA of 0.025 M to 2 M, which was broader than physiological ionic strength (0.14 M) and urinary NaCl (0.17 M). Our results demonstrated that this graphitic sheet based SERS detection platform can be used to determine BPA levels leached from commercial polycarbonate plastic products and for on-site rapid analysis with good results.
Medical examination powers miR-194-5p as a biomarker for postmenopausal osteoporosis
Authors: Ding Haifeng , Meng Jia , Zhang Wei , Li Zhangming , Li Wenjing , Zhang Mingming , Fan Ying , Wang Qiujun , Zhang Yina , Jiang Lihong , Zhu Wenliang 
Published: November 2017

AbstractAn important attribute of microRNAs is their potential use as disease biomarkers. However, such applications may be restricted because of unsatisfactory performance of the microRNA of interest. Owing to moderate correlation with spine T-score, miR-194-5p was identified as a potential biomarker for postmenopausal osteoporosis. Here, we determined whether medical examination could improve its characteristic as a biomarker for postmenopausal osteoporosis. We recruited 230 postmenopausal Chinese women to measure circulating levels of miR-194-5p, determine the spine bone status, and perform a 42-item medical examination. No obvious information redundancy was observed between miR-194-5p and any one item. However, on examining miR-194-5p alone, the sensitivity at fixed specificity of 0.9 (SESP=0.9) was 0.27, implying poor identification of at-risk individuals. Model integration of the microRNA and multiple medical items strengthened this property; in addition, model complexity greatly contributed to performance improvement. Using a model composed of two artificial neural networks, the ability of miR-194-5p to identify at-risk individuals significantly improved (SESP=0.9 = 0.54) when correlated with five medical items: weight, age, left ventricular end systolic diameter, alanine aminotransferase, and urine epithelial cell count. We present a feasible way to achieve a more accurate microRNA-based biomarker for a disease of interest.
Magnetic stratigraphic dating of marine hydrogenetic ferromanganese crusts
Authors: Yuan Wei , Zhou Huaiyang , Zhao Xixi , Yang Zhenyu , Yang Qunhui , Zhu Benduo 
Published: November 2017

AbstractDeep-sea hydrogenetic ferromanganese crusts are both potential polymetallic resources and records of long-term environmental changes. For palaeoceanographic studies, it is important to construct a detailed and reliable chronological framework. Here, we report the results of a detailed magnetostratigraphic and rock magnetic study of four hydrogenetic Fe-Mn crusts from the Pacific Ocean (PO-01), South China Sea (SCS-01, SCS-02) and Indian Ocean (IO-01). Two groups of characteristic remanent magnetization directions were defined with nearly antipodal normal and reversed polarities for samples PO-01, SCS-01 and SCS-02, indicating a primary record of the Earth’s magnetic field. The magnetostratigraphic framework, established via correlation with the Geomagnetic Polarity Time Scale 2012, implies growth rates of 4.82 mm/Ma, 4.95 mm/Ma, 4.48 mm/Ma and 11.28 mm/Ma for samples PO-01, SCS-01, SCS-02 and IO-01, respectively. Rock magnetic measurements revealed that the Fe-Mn crust samples from the Pacific Ocean and Indian Ocean were dominated by low coercivity, non-interacting, single-domain (SD) magnetite particles, whereas the South China Sea samples were dominated by SD/pseudo-single-domain (PSD) particles. Multidomain (MD) magnetite may also be present in all samples.
Legume crop rotation suppressed nitrifying microbial community in a sugarcane cropping soil
Authors: Paungfoo-Lonhienne Chanyarat , Wang Weijin , Yeoh Yun Kit , Halpin Neil 
Published: November 2017

AbstractNitrifying microorganisms play an important role in nitrogen (N) cycling in agricultural soils as nitrification leads to accumulation of nitrate (NO3−) that is readily lost through leaching and denitrification, particularly in high rainfall regions. Legume crop rotation in sugarcane farming systems can suppress soil pathogens and improve soil health, but its effects on soil nitrifying microorganisms are not well understood. Using shotgun metagenomic sequencing, we investigated the impact of two legume break crops, peanut (Arachis hypogaea) and soybean (Glycine max), on the nitrifying communities in a sugarcane cropping soil. Cropping with either legume substantially increased abundances of soil bacteria and archaea and altered the microbial community composition, but did not significantly alter species richness and evenness relative to a bare fallow treatment. The ammonia oxidisers were mostly archaeal rather than bacterial, and were 24–44% less abundant in the legume cropping soils compared to the bare fallow. Furthermore, abundances of the archaeal amoA gene encoding ammonia monooxygenase in the soybean and peanut cropping soils were only 30–35% of that in the bare fallow. These results warrant further investigation into the mechanisms driving responses of ammonia oxidising communities and their nitrification capacity in soil during legume cropping.
The role of oxidative stress in the crosstalk between leptin and mineralocorticoid receptor in the cardiac fibrosis associated with obesity
Authors: Gutiérrez-Tenorio Josué , Marín-Royo Gema , Martínez-Martínez Ernesto , Martín Rubén , Miana María , López-Andrés Natalia , Jurado-López Raquel , Gallardo Isabel , Luaces María , San Román José Alberto , González-Amor María , Salaices Mercedes , Nieto María Luisa , Cachofeiro Victoria 
Published: November 2017

AbstractWe have investigated whether mineralocorticoid receptor activation can participate in the profibrotic effects of leptin in cardiac myofibroblasts, as well as the potential mechanisms involved. The presence of eplerenone reduced the leptin-induced increase in protein levels of collagen I, transforming growth factor β, connective tissue growth factor and galectin-3 and the levels of both total and mitochondrial of superoxide anion (O2.−) in cardiac myofibroblasts. Likewise, the MEK/ERK inhibitor, PD98059, and the PI3/Akt inhibitor, LY294002, showed a similar pattern. Mitochondrial reactive oxygen species (ROS) scavenger (MitoTempo) attenuated the increase in body weight observed in rats fed a high fat diet (HFD). No differences were found in cardiac function or blood pressure among any group. However, the cardiac fibrosis and enhanced O2.-levels observed in HFD rats were attenuated by MitoTempo, which also prevented the increased circulating leptin and aldosterone levels in HFD fed animals. This study supports a role of mineralocorticoid receptor in the cardiac fibrosis induced by leptin in the context of obesity and highlights the role of the mitochondrial ROS in this process.
Cascading collapse of online social networks
Authors: Török János , Kertész János 
Published: November 2017

AbstractOnline social networks have increasing influence on our society, they may play decisive roles in politics and can be crucial for the fate of companies. Such services compete with each other and some may even break down rapidly. Using social network datasets we show the main factors leading to such a dramatic collapse. At early stage mostly the loosely bound users disappear, later collective effects play the main role leading to cascading failures. We present a theory based on a generalised threshold model to explain the findings and show how the collapse time can be estimated in advance using the dynamics of the churning users. Our results shed light to possible mechanisms of instabilities in other competing social processes.
Transcriptome analysis of watermelon (Citrullus lanatus) fruits in response to Cucumber green mottle mosaic virus (CGMMV) infection
Authors: Li Xiaodong , An Mengnan , Xia Zihao , Bai Xiaojiao , Wu Yuanhua 
Published: November 2017

AbstractCucumber green mottle mosaic virus(CGMMV) belongs to theTobamovirusgenus and is a major global plant virus on cucurbit plants. It causes severe disease symptoms on infected watermelon plants (Citrullus lanatus), particularly inducing fruit decay. However, little is known about the molecular mechanism of CGMMV-induced watermelon fruit decay. For this study, comparative analysis of transcriptome profiles of CGMMV-inoculated and mock-inoculated watermelon fruits were conducted via RNA-Seq. A total of 1,621 differently expressed genes (DEGs) were identified in CGMMV-inoculated watermelon, among which 1,052 were up-regulated and 569 were down-regulated. Functional annotation analysis showed that several DEGs were involved in carbohydrate metabolism, hormone biosynthesis and signaling transduction, secondary metabolites biosynthesis, and plant-pathogen interactions. We furthermore found that some DEGs were related to cell wall components and photosynthesis, which may directly be involve in the development of the symptoms associated with diseased watermelons. To confirm the RNA-Seq data, 15 DEGs were selected for gene expression analysis by qRT-PCR. The results showed a strong correlation between these two sets of data. Our study identified many candidate genes for further functional studies during CGMMV-watermelon interactions, and will furthermore help to clarify the understanding of pathogenic mechanism underlying CGMMV infection in cucurbit plants.
High-pressure versus low-pressure home non-invasive positive pressure ventilation with built-in software in patients with stable hypercapnic COPD: a pilot study
Authors: Zhou Luqian , Guan Lili , Wu Weiliang , Li Xiaoying , Chen Xin , Guo Bingpeng , Huo Yating , Xu Jiawen , Yang Yuqiong , Chen Rongchang 
Published: November 2017

AbstractHigh-pressure non-invasive positive pressure ventilation (NPPV) is a new strategy targeted at maximally reducing arterial carbon dioxide. However, high inspiratory positive airway pressure (IPAP) might cause respiratory adverse events likely to diminish the benefit of NPPV. In the setting of ventilatory support, monitoring NPPV efficacy and resolving problems promptly are critical. This study assessed the treatment effect of high and low-pressure NPPV in chronic hypercapnic COPD using home ventilator with built-in software. In this pilot study, we investigated 34 patients using NPPV for 3 months. 13 patients used high-pressure ventilation and 21 patients used low-pressure ventilation. The primary outcome was daytime partial pressure of arterial blood carbon dioxide (PaCO2). There were no between-group differences in daytime PaCO2 and FEV1, but a trend favouring high-pressure NPPV was observed. Significant between-group differences were found in the transition dyspnoea index (TDI) (high-pressure, 1.69 ± 1.75, versus low-pressure, −0.04 ± 2.71, p = 0.044). No differences were found in usage time, leakage, health-related quality of life, spirometry, or 6-minute walk test. High-pressure NPPV with built-in software monitoring in patients with chronic hypercapnic COPD is associated with improvement in TDI scores and a positive trend in favour of high-pressure NPPV for improving PaCO2 is observed.
Is There Any Reason Not to Perform Standard Laparoscopic Total Mesorectal Excision?
Authors: Lakkis Zaher , Panis Yves 
Published: November 2017

AbstractThe curative treatment of locally advanced rectal cancer is currently based on chemoradiotherapy and total mesorectal excision (TME). Laparoscopy has developed considerably because of obvious clinical benefits such as reduced pain and shorter hospital stay. Recently, several prospective randomized clinical trials with long-term follow-up have showed that laparoscopy is noninferior to laparotomy with the same oncologic outcomes in terms of survival and local control rate. However, laparoscopic TME remains a challenging procedure requiring a high level of expertise and a long learning curve to ensure an adequate and safe resection. The only relative contraindication of laparoscopic rectal surgery is T4 rectal cancer extended beyond the plane of TME. In this situation, it is reasonable to consider an open resection to avoid an uncomplete resection. In obese and elderly patients, laparoscopic TME also provides the same benefits as in nonobese and younger patients but may be more difficult to achieve. This review summarizes current knowledge on the place of laparoscopic TME in the treatment of rectal cancer.
Transanal Total Mesorectal Excision: Why, When, and How
Authors: Penna Marta , Cunningham Christopher , Hompes Roel 
Published: November 2017

AbstractTransanal total mesorectal excision (taTME) has evolved over the past decade fueled by advances in minimally invasive surgery. The technique aims to overcome the constraints posed by a narrow rigid pelvis and poor TME visualization that are encountered during “top-down” rectal surgery. A more accurate pelvic dissection should subsequently result in safer oncological resections and better preservation of pelvic autonomic nerves. taTME is an advanced complex technique that requires dedicated training and experience in TME surgery. Initial results from small cohorts are promising and confirmation by randomized controlled trials is eagerly awaited.