bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smart grid line fault detection based on deep learning image recognition algorithm
Authors: Huang Jianfeng , Wan Qiang 
Published: September 2024

Abstract
Amid the rapid evolution of smart grids, stringent demands for reliability, safety, and efficiency have escalated for transmission lines. Nevertheless, given their extensive coverage and the complexity of their environments, traditional inspection techniques struggle to meet the rigorous standards of real-time monitoring and precision. This paper introduces a novel fault detection approach for smart grid transmission lines, leveraging advanced deep-learning image recognition algorithms. This method improves the YOLOv5 series models by combining the Convolution Attention Module (CBAM), Bidirectional Feature Pyramid Network (BiFPN), and MobileNet-V3 as the feature extraction network of YOLOv5 to achieve defect detection of hardware on transmission lines. CBAM improves the model’s sensitivity to tiny defects by focusing on key areas and features. BiFPN improves detection accuracy and robustness through efficient fusion of multi-scale features. Furthermore, the integration of MobileNet-V3 enhances both the efficiency and precision of feature extraction, ultimately elevating the overall model’s performance. Experimental results show that compared with the original YOLOv5 model, the improved algorithm can achieve 92.89% accuracy and 84.10% recall, which is better than other mainstream target detection algorithms. Especially in the detection of defects in small targets and complex backgrounds, the improved model shows stronger robustness and adaptability. This provides strong technical support for the efficient operation and maintenance of smart grids.
Impact of transferable β-lactamases and intrinsic AmpC amino acid substitutions on the activity of cefiderocol against wild-type and iron uptake-deficient mutants of Pseudomonas aeruginosa
Authors: González-Pinto Lucía , Blanco-Martín Tania , Alonso-García Isaac , Rodríguez-Pallares Salud , Outeda-García Michelle , Gomis-Font María Antonia , Fraile-Ribot Pablo Arturo , Vázquez-Ucha Juan Carlos , González-Bello Concepción , Beceiro Alejandro , Oliver Antonio , Bou Germán , Arca-Suárez Jorge 
Published: September 2024

Abstract

Objectives
We aimed to analyse the interplay between impaired iron uptake and β-lactamases on cefiderocol resistance in Pseudomonas aeruginosa.

Methods
Thirty-one transferable β-lactamases and 16 intrinsic P. aeruginosa AmpC (PDC) variants were cloned and expressed in wild-type (PAO1) and iron uptake-deficient (PAO ΔpiuC) P. aeruginosa backgrounds. MICs of cefiderocol and antipseudomonal β-lactams were determined by reference broth microdilution.

Results
Relative to PAO1, deletion of piuC caused a specific 16-fold decrease in cefiderocol activity but negligible effects on the activity of other β-lactams. Among transferable β-lactamases, SHV-12, KPC Ω-loop mutants, NDMs and OXA-15 showed cefiderocol MIC values above the clinical breakpoint (2 mg/L) when expressed in PAO1. When expressed in PAO ΔpiuC, these and the transformants harbouring PER-1, VEB-1, KPC-2, KPC-3, VIM-1, CMY-2, OXA-2 and OXA-14 showed increased MIC values from 16 to &gt;256 mg/L. The PDC variants carrying the Ω-loop changes ΔP215-G222 (PDC-577), E219K (PDC-221 and PDC-558) and the H10 helix change L293P (PDC-219) had the greatest impact on cefiderocol resistance, with MICs of 2–4 mg/L in PAO1 and of up to 32–64 mg/L in PAO ΔpiuC. Widespread enzymes such as GES, CTX-M-9, CTX-M-15, VIM-2-like enzymes, IMPs, DHA-1, FOX-4, OXA-10, OXA-48 and the other PDC variants tested had weaker effects on cefiderocol resistance.

Conclusion
We add evidence about the effect of the interplay between iron uptake and β-lactamases on the acquisition of cefiderocol resistance in P. aeruginosa. These findings may help to anticipate the emergence of resistance and optimize the use of cefiderocol against P. aeruginosa infections.
Susceptibility of Gram-negative pathogens collected in Israel to ceftolozane/tazobactam, imipenem/relebactam and comparators: SMART 2018–22
Authors: Wise Mark G , DeRyke C Andrew , Alekseeva Irina , Siddiqui Fakhar , Young Katherine , Motyl Mary R , Sahm Daniel F 
Published: September 2024

Abstract

Objectives
To assess the in vitro antimicrobial activity of ceftolozane/tazobactam, imipenem/relebactam and comparator agents against clinical isolates of Gram-negative bacilli collected in Israel from 2018 to 2022.

Methods
Six clinical laboratories each collected up to 250 consecutive Gram-negative isolates per year from patients with bloodstream, intra-abdominal, lower respiratory tract and urinary tract infections. MICs were determined by CLSI broth microdilution and interpreted with 2024 EUCAST breakpoints. Acquired β-lactamase gene carriage was investigated for most ceftolozane/tazobactam- and imipenem/relebactam-resistant isolates.

Results
Among the full collection of Enterobacterales (n = 4420), 95.1% were susceptible to ceftolozane/tazobactam, including 95.3% of putative AmpC/ESBL-positive, non-carbapenem-resistant Enterobacterales (CRE) phenotype Escherichia coli and 86.6% of AmpC/ESBL-positive, non-CRE phenotype Klebsiella pneumoniae. Overall, 99.8% of non-Morganellaceae Enterobacterales (n = 3723) were imipenem/relebactam susceptible including 98% of the MDR isolates. Most Pseudomonas aeruginosa isolates (n = 1182) were inhibited by ceftolozane/tazobactam (93.9% susceptible) and imipenem/relebactam (94.7%). Imipenem/relebactam retained activity against ≥78% of cefepime-resistant, ceftazidime-resistant, and piperacillin/tazobactam-resistant P. aeruginosa, while ceftolozane/tazobactam inhibited the greatest percentage of meropenem-resistant P. aeruginosa (67.4%) among comparator β-lactam antimicrobials. Molecular characterization showed the majority of imipenem/relebactam-resistant Enterobacterales harboured a metallo-β-lactamase, while half of the ceftolozane/tazobactam-resistant Enterobacterales carried an acquired ESBL or AmpC. Most of the imipenem/relebactam- and ceftolozane/tazobactam-resistant P. aeruginosa characterized did not possess acquired β-lactamases.

Conclusions
Recent clinical isolates of Enterobacterales and P. aeruginosa collected in Israel were highly susceptible to ceftolozane/tazobactam and imipenem/relebactam.
Comparison of Roka Atlas® System Performance and Health Canada Reference Method for Listeria Detection From Plastic, Sealed Concrete, and Stainless-Steel Surface Samples
Authors: Forghani Fereidoun , Themeli Eni , Mai Tam , Samadpour Mansour 
Published: September 2024

Abstract

Background
There is a high demand in the food industry and in public health for rapid automated methods capable of high-volume sample processing.

Objective
In an unpaired study, Roka Atlas® System performance was compared with Health Canada reference method MFHPB-30 for Listeria spp. (LSP Roka assay) and Listeria monocytogenes (LmG2 Roka Assay) detection on plastic (PL), sealed concrete (SC), and stainless-steel (SS) surfaces (45 samples each per candidate or reference method).

Methods
Seeking shorter enrichment time for the candidate method, R2 medium pre-enrichment for 14, 16, and 24 h at 35°C was combined with the Roka assay. Listeria welshimeri, L. innocua, and L. monocytogenes were employed to individually inoculate each of the three surfaces, with two competing microorganisms within the 10–100-fold higher concentration range.

Results
False negative, false positive, sensitivity, and specificity were 0, 0, 100, and 100%, respectively, for the plastic, sealed concrete, and stainless-steel surfaces, regardless of inoculation level (high, low, and uninoculated) and enrichment time. Candidate method detected 10, 7, and 9 true positives, versus 10, 6, and 10 by the reference method in individually inoculated SS, PL, and SC, respectively.

Conclusions
Probability of detection for all the three surfaces for the Roka Atlas System was comparable to the reference method in this unpaired study.

Highlights
The Roka Atlas System detected targets after as little as 14 h enrichment. Surface type did not negatively affect assay sensitivity or specificity. The Roka Atlas System was comparable to the reference method.
Zearalenone triggers programmed cell death and impairs milk fat synthesis via the AKT-mTOR-PPARγ-ACSL4 pathway in bovine mammary epithelial cells
Authors: Ma Zifeng , Li Qiao , Xu Hongmei , Li Yueyue , Wang Shujin , Xiong Yan , Lan Daoliang , Li Jian , Xiong Xianrong , Fu Wei 
Published: September 2024

Abstract
Zearalenone (ZEN), a mycotoxin from Fusarium fungi, impairs fertility and milk production in female animals; however, the mechanisms remain poorly understood. Using the bovine mammary epithelial cells (MAC-T) as the model, this study investigated the impacts of ZEN on programmed cell death (PCD) and milk fat synthesis and explored the underlying mechanism. We found that 10 ng/mL prolactin (PRL) notably enhanced the differentiation of MAC-T cells, promoting the expression of genes related to the synthesis of milk fat, protein, and lactose. Next, the toxic effects of different doses of ZEN on the differentiated MAC-T with PRL treatment were determined. 10 and 20 μM ZEN significantly reduced cell viability, induced oxidative stress, and triggered PCD (e.g., apoptosis and necrosis). Notably, ZEN exposure downregulated the mRNA/protein levels of critical factors involved in milk fat synthesis by disrupting the AKT-mTOR-PPARγ-ACSL4 pathway. Interestingly, melatonin (MT), known for its antioxidant properties, protected against the above ZEN-induced effects by enhancing the binding of PPARγ to the promoter regions of ACSL4, which led to the upregulated expression of the ACSL4 gene. These results underscored the potential of MT to mitigate the adverse effects of ZEN on mammary cells, highlighting a way for potential therapeutic intervention.
Fighting a New Front on an Old Battlefield: Examining the Development of Topical Antimicrobial Care to Control Burn Wound Sepsis
Authors: Tejiram Shawn , Shupp Jeffrey W 
Published: September 2024

Abstract
Recognition of invasive burn wound sepsis as a major cause of morbidity and mortality in burn-injured patients has profoundly changed the management of burn wounds and its associated complications. The development of effective topical antimicrobial therapy is one of the last major developments of modern burn care and has been driven by major world events and scientific breakthroughs. Topical antimicrobial burn care has evolved from the use of anecdotal remedies to scientific breakthroughs such as Moyer’s successful dilution of silver nitrate solution, Fox’s described benefit of silver sulfadiazine use in animal models, and Pruitt’s dramatic improvement in post-burn mortality using topical mafenide acetate in burn wounds. The objective of this manuscript is to review the definition of burn wound sepsis and highlight the major developments and breakthroughs in topical burn wound care throughout history. This includes historical events like major wars or domestic fires that have influenced or impacted the understanding and treatment of burn wounds. Newer advances in topical antimicrobial care such as nanosilvers and dressing technologies that improve the morbidity and mortality associated with burn wound sepsis and novel approaches to management will also be discussed. To improve burn care, it is prudent to look to the past and learn from the experiences of those who contributed to the control of burn wound sepsis.
Friction Burns: Defining the Rub of Road Rash After Motorcycle Trauma
Authors: Perkins Louis A , Munter Sadie , Johnston William , Lee Jeanne G , Haines Laura N , Doucet Jay J , Smith Alan , Santorelli Jarrett E 
Published: September 2024

Abstract
There is a paucity of literature on friction burn epidemiology, injury characteristics, and management after trauma. This study aims to characterize friction burns resulting from motorcycle accidents, evaluate the role of specialized burn surgery teams, and assess the need for operative intervention. The trauma registry of a Level 1 Trauma Center was queried for all admissions after motorcycle accidents between January 2018 and December 2022. Patients were included if there was an external cause of injury code for an abrasion. Chart review was conducted to confirm a road rash injury, identify burn consultation, wound care recommendations, need for operating room (OR) procedure and skin grafting, and outcomes. Among the 810 patients meeting inclusion criteria, the cohort was 92% male and 46% Hispanic, with a median age of 33 (IQR 26-44) and a median injury severity score of 8 (IQR 5-14). The extremities were most affected by friction burn, followed by the abdomen, thorax, and face. Burn surgery was consulted in 7% (n = 57) of cases which had a median TBSA of 4% (IQR 2%-6%); 23% (n = 13) of these patients underwent excision in the OR, 16% (n = 9) required autografting, and 61% (n = 35) were referred for burn follow-up on discharge. On multivariable regression, consults to burn were more likely in female patients (OR 3.40, P = .002) and those with involvement of the upper extremity, abdomen, or thorax. Friction burns are common after motorcycle-related trauma. Local wound care is sufficient for a vast majority of these injuries and the need for specialized burn care and operative intervention is rare.
Burn Care in the Street: A Survey of the Current Landscape of Burn Care Provided by Street Medicine Teams
Authors: Ross Erin E , Coulourides Kogan Alexis , Johnson Maxwell B , Yenikomshian Haig A 
Published: September 2024

Abstract
People experiencing homelessness are at increased risk for serious burn injuries and face additional barriers to care such as inability to perform wound care and difficulty with follow-up. Although not burn-specific, street medicine (SM) programs provide direct medical care to people experiencing unsheltered homelessness in their own environment and may be well positioned to bridge this gap in burn care for this population. We conducted a cross-sectional survey to characterize the burn care experience of SM teams with providing burn care for people experiencing homelessness. The 60 respondents included 18 (30%) physicians, 15 (25%) nurse practitioners or physician assistants, 15 (25%) registered nurses, 6 (10%) medical students, and 6 (10%) other team members. The most common reported barriers to care were prior negative experiences with emergency departments, and transportation to burn centers. There was regional variability in frequency of providing burn care, which was reflected in respondent comfort with assessing and treating burns. Burns were most often dressed with daily dressings such as a non-adherent dressing over silver sulfadiazine or bacitracin. Silver-based contact dressings were rarely used. Street medicine teams in the United States are treating burn injuries among people experiencing homelessness, though management practices and experience treating burns was variable. As SM programs continue to grow, burn-related education, training, and connections to local burn centers for team members is important. Through strengthened partnerships between burn centers and street medicine teams, these programs may be well positioned to bridge the gap in burn care for people experiencing homelessness.
Differential microRNA expression patterns between TallyHo/JngJ mice and non-diabetic Swiss Webster Random/Jackson mice
Authors: Carro Vázquez David , Emini Lejla , Rauner Martina , Hofbauer Christine , Skalicky Susanna , Wagner Alisia , Grillari Johannes , Diendorfer Andreas B , Hofbauer Lorenz C , Hackl Matthias 
Published: September 2024

Abstract
Type 2 diabetes mellitus (T2DM) increases the susceptibility of bone fragility. The underlying mechanisms have, however, remained largely unknown. MicroRNAs (miRNAs) are short single-stranded non-coding RNA molecules with utility as biomarkers due to their easy accessibility and stability in bodily fluids. Here, we aimed to use an unbiased approach to identify miRNAs dysregulated in a polygenic mouse model of T2DM. Genome-wide analysis of miRNAs in serum, BM, and bone from the polygenic TallyHo/JngJ (TH) mice, which recapitulate T2DM in humans, was performed. This analysis was compared to the recommended control Swiss Webster Random/Jackson (SWR/J) and a strain-matched non-diabetic control (TH-ND). When comparing TH mice with TH-ND using an adjusted p-value false discovery rate (FDR) cut-off of 0.2 to identify differentially expressed miRNAs, mmu-miR-466i-5p and mmu-miR-1195 were found to be up-regulated in both serum and in BM. Dysregulated miRNAs were not found in bone tissue. When comparing TH-ND mice with SWR/J using the same FDR cut-off, mmu-miR-351-5p, and mmu-miR-322-3p were upregulated in both BM and serum, while mmu-miR-449a-5p and mmu-miR-6240 were downregulated in BM and serum. Dysregulated miRNAs in BM or cortical bone compared to serum between TH-ND mice and SWR/J were investigated for their cell-type enrichment to identify putative donor cells and their gene target networks. Gene target network analysis revealed genes involved in diabetes-related signaling pathways as well as in diabetic bone disease. Cell-type enrichment analysis identified hsa-miR-449a enriched in immune cells, hsa-miR-592 in hepatocytes and endothelial cells, while hsa-miR-424-3p, hsa-miR-1-3p, and hsa-miR-196b-5p were enriched in mesenchymal stem cells and their derived tissues. In conclusion, our comparative miRNA profiling sheds light on differential expression patterns between SWR/J and both subgroups of TH. No differences were observed between TH and TH-ND, suggesting the genetic background of SWR/J may be responsible for the change of dysregulated miRNA.
Nutrition affects larval survival and the development of morphological traits in male and female flour beetles, but genital size and shape remains canalised
Authors: House Clarissa M , Rapkin James , Janicot Bale Mathilda , Hunt John , Hosken David J 
Published: September 2024

Abstract
The caloric content and macronutrient ratio of diet consumed is a major source of phenotypic variation in most animal populations. While these nutritional effects have been well-documented for a variety of life-history and morphological traits, the effects of nutrition on male genitals are poorly understood but genitals are thought to be more canalised than general morphology and hence less susceptible to variation in nutrition. Even less is known about the effects of nutrition on female genital form, which to our knowledge, have never been investigated. Here we tested for effects of juvenile dietary macronutrients (protein and carbohydrate) on larval survival, adult morphology, including genital size and shape in male and female flour beetles (Tribolium castaneum). We found there was nutritionally induced plasticity in larval survival and morphology, although the latter effect was variable, with body size being most responsive to dietary macronutrients and genital size and shape being least responsive. Functionally equivalent morphological traits in the sexes responded similarly to nutrition. Previously, we showed that the genitalia of male and female T. castaneum are subject to strong stabilising sexual selection, and our current findings suggest that developmental mechanisms reduce the nutritional sensitivity of male and female genitals, possibly to ensure matching during mating.
Linking divergence in phenotypic selection on floral traits to divergence in local pollinator assemblages in a pollination-generalized plant
Authors: Torres-Vanegas Felipe , Temesvári Vanda , Hildesheim Laura S , Rodríguez-Otero Cristina , Müller Vilhelmina , Aukema Easger , Friberg Magne , Opedal Øystein H 
Published: September 2024

Abstract
Divergent patterns of phenotypic selection on floral traits can arise in response to interactions with functionally distinct pollinators. However, there are a limited number of studies that relate patterns of phenotypic selection on floral traits to variation in local pollinator assemblages in pollination-generalized plant species. We studied phenotypic selection on floral traits of Viscaria vulgaris, a plant that interacts with a broad range of diurnal and nocturnal pollinators, and related divergence in phenotypic selection on floral traits to the expected level of divergence in local pollinator assemblages. We detected phenotypic selection on floral traits involved in the attraction of pollinators and the mechanics of pollen removal and deposition, and demonstrated that floral traits are subject to spatiotemporal variation in the strength and direction of phenotypic selection. We revealed that diurnal and nocturnal pollinators, when considered in isolation, mediated divergent patterns of phenotypic selection on floral traits. Consistent with the Grant–Stebbins model, we observed that divergence in phenotypic selection on floral traits increased with the expected level of divergence in local pollinator assemblages. Thus, generalized plant–pollinator interactions can mediate phenotypic selection on floral traits, and distinct local pollinator assemblages can generate a geographic mosaic of divergent patterns of phenotypic selection. We underscore that these outcomes are not exclusive to specialized plant–pollinator interactions and can emerge at a local geographic scale.
Invasive wild boar affected feral hog skull evolution over 25 years in Brazilian Pantanal wetland
Authors: Sicuro Fernando L , de Oliveira Luiz Flamarion B , Ferreira Eduardo , Rocha-Barbosa Oscar 
Published: September 2024

Abstract
The morphological plasticity of the Sus scrofa wild–domestic species complex is evident in both free-ranging morphotypes and domestic breeds. The Brazilian Pantanal feral hog (“porco-monteiro”) evolved after a long-term feralization process and represents a significant proportion of the mammalian biomass in the region. Its coexistence with native fauna brings several ecological impacts, which are mitigated by the Brazilian Pantanal wetland vastness and resource availability. They are a local subsistence game resource but also impact crops and pastures. Around the 2000s to mid-2010s, numerous European wild boar were introduced in Brazil to fulfill a demand for gourmet meat. Wild boar was also introduced to the Brazilian Pantanal as a game species and to breed with porco-monteiro feral hogs in a commercial venture to produce an even more exotic meat. The craze for wild boar meat has since dwindled, but their populations remain widespread and uncontrolled. Moreover, the full impact of the wild boar introduction on feral hog populations is still unknown. This study analyzes the skulls of porco-monteiro feral hogs from the 1990s, 2000s, and 2010s to assess possible morphological and functional variations along with skulls of wild boar and hybrids from Argentina, Uruguay, and Southern Brazil. The results indicate a trend of broadening of feral hog skulls at the coronal plane and a progressive reduction of the semispinalis capitis muscle scars over the decades. Biomechanical analysis denoted a decrease in head-elevation force that could affect the rooting performance of the latest feral hog morphotypes. Taken together, results show that wild boars likely influenced the evolution of the Pantanal feral hog skull. The magnitude of ecomorphological implications of these morphofunctional changes is unknown, as well as its effects in the Brazilian Pantanal wetland ecology.