bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Screening Identifies EUrd as a Novel Inhibitor Against Temozolomide-Resistant Glioblastoma-Initiating Cells
Authors: Tsukamoto Yoshihiro , Ohtsu Naoki , Echizenya Smile , Otsuguro Satoko , Ogura Ryosuke , Natsumeda Manabu , Isogawa Mizuho , Aoki Hiroshi , Ichikawa Satoshi , Sakaitani Masahiro , Matsuda Akira , Maenaka Katsumi , Fujii Yukihiko , Kondo Toru 
Published: April 2016

Abstract
Glioblastoma (GBM), one of the most malignant human cancers, frequently recurs despite multimodal treatment with surgery and chemo/radiotherapies. GBM-initiating cells (GICs) are the likely cell-of-origin in recurrences, as they proliferate indefinitely, form tumors in vivo, and are resistant to chemo/radiotherapies. It is therefore crucial to find chemicals that specifically kill GICs. We established temozolomide (the standard medicine for GBM)-resistant GICs (GICRs) and used the cells for chemical screening. Here, we identified 1-(3-C-ethynyl-β-d-ribopentofuranosyl) uracil (EUrd) as a selective drug for targeting GICRs. EUrd induced the death in GICRs more effectively than their parental GICs, while it was less toxic to normal neural stem cells. We demonstrate that the cytotoxic effect of EUrd on GICRs partly depended on the increased expression of uridine-cytidine kinase-like 1 (UCKL1) and the decreased one of 5′-nucleotidase cytosolic III (NT5C3), which regulate uridine-monophosphate synthesis positively and negatively respectively. Together, these findings suggest that EUrd can be used as a new therapeutic drug for GBM with the expression of surrogate markers UCKL1 and NT5C3.
Brief Report: Alternative Splicing of Extra Domain A (EIIIA) of Fibronectin Plays a Tissue-Specific Role in Hematopoietic Homeostasis
Authors: Malara Alessandro , Gruppi Cristian , Celesti Giuseppe , Romano Bina , Laghi Luigi , Marco Luigi , Muro Andrés F. , Balduini Alessandra 
Published: April 2016

Abstract
Fibronectin (FN) is a major extracellular matrix protein implicated in cell adhesion and differentiation in the bone marrow (BM) environment. Alternative splicing of FN gene results in the generation of protein variants containing an additional EIIIA domain that sustains cell proliferation or differentiation during physiological or pathological tissue remodeling. To date its expression and role in adult hematopoiesis has not been explored. In our research, we demonstrate that during physiological hematopoiesis a small fraction of BM derived FN contains the EIIIA domain and that mice constitutively including (EIIIA+/+) or excluding (EIIIA−/−) the EIIIA exon present comparable levels of hematopoietic stem cells, myeloid and lymphoid progenitors within BM. Moreover, only minor alterations were detected in blood parameters and in hematopoietic frequencies of BM granulocytes/monocytes and B cells. As opposed to other tissues, unique compensatory mechanisms, such as increased FN accumulation and variable expression of the EIIIA receptors, Toll like receptor-4 and alpha9 integrin subunit, characterized the BM of these mice. Our data demonstrate that FN is a fundamental component of the hematopoietic tissue and that the EIIIA exon may play a key role in modulating hematopiesis in conditions of BM stress or diseases.
Neuropeptide Y Induces Hematopoietic Stem/Progenitor Cell Mobilization by Regulating Matrix Metalloproteinase-9 Activity Through Y1 Receptor in Osteoblasts
Authors: Park Min Hee , Lee Jong Kil , Kim Namoh , Min Woo-Kie , Lee Jeong Eun , Kim Kyoung-Tae , Akiyama Haruhiko , Herzog Herbert , Schuchman Edward H. , Jin Hee Kyung , Bae Jae-sung 
Published: April 2016

AbstractHematopoietic stem/progenitor cell (HSPC) mobilization is an essential homeostatic process regulated by the interaction of cellular and molecular components in bone marrow niches. It has been shown by others that neurotransmitters released from the sympathetic nervous system regulate HSPC egress from bone marrow to peripheral blood. In this study, we investigate the functional role of neuropeptide Y (NPY) on this process. NPY deficient mice had significantly impaired HSPC mobilization due to increased expression of HSPC maintenance factors by reduction of matrix metalloproteinase-9 (MMP-9) activity in bone marrow. Pharmacological or endogenous elevation of NPY led to decrease of HSPC maintenance factors expression by activating MMP-9 in osteoblasts, resulting in HSPC mobilization. Mice in which the Y1 receptor was deleted in osteoblasts did not exhibit HSPC mobilization by NPY. Furthermore, NPY treatment in ovariectomized mice caused reduction of bone loss due to HSPC mobilization. These results suggest a new role of NPY on HSPC mobilization, as well as the potential therapeutic application of this neuropeptide for stem cell-based therapy.
Perinatal and long‐term outcomes in fetuses diagnosed with isolated unilateral ventriculomegaly: systematic review and meta‐analysis
Authors: Scala C. , Familiari A. , Pinas A. , Papageorghiou A. T. , Bhide A. , Thilaganathan B. , Khalil A. 
Published: April 2016

ABSTRACTObjectivesThe majority of studies on fetal ventriculomegaly have focused on the perinatal and long‐term outcomes in fetuses with an antenatal diagnosis of bilateral ventriculomegaly. The aim of this study was to undertake a systematic review and meta‐analysis to quantify the perinatal and long‐term outcomes in fetuses diagnosed in the second or third trimester of pregnancy with isolated unilateral ventriculomegaly.MethodsMEDLINE, EMBASE and The Cochrane Library were searched electronically. Outcomes investigated included incidence of aneuploidy, congenital infection, progression of ventriculomegaly, associated brain and extracerebral abnormalities in the apparently isolated cases and neurodevelopmental delay in both apparently and truly isolated cases. Sensitivity analysis was performed according to whether the ventriculomegaly was mild/moderate (atrial width < 15 mm) or severe (atrial width ≥ 15 mm). Reference lists within relevant articles and reviews were hand‐searched for additional reports. Cohort and case–control studies were included. Meta‐analysis of proportions was used, and between‐study heterogeneity was assessed using the I2 test.ResultsThe search yielded 2053 citations. The full text was retrieved for 202, and 11 studies were included in the systematic review. In fetuses with apparently isolated unilateral ventriculomegaly, no chromosomal abnormalities were identified and the pooled prevalence of congenital infection was 8.2% (95% CI, 3.6–14.5%). The pooled prevalence of additional brain abnormalities detected prenatally and postnatally by magnetic resonance imaging was 5.1% (95% CI, 0.2–16.1%) and 6.4% (95% CI, 0.3–19.4%), respectively. The pooled prevalence of abnormal neurodevelopment was 5.9% (95% CI, 2.2–11.2%) in apparently isolated cases with an atrial width of < 15 mm, and it was 7.0% (95% CI, 3.2–12.2%) in fetuses with truly isolated unilateral ventriculomegaly. Most cases with apparently isolated ventriculomegaly were classified as mild/moderate (93.5%) and therefore the outcomes in this group were similar to those in the whole cohort of apparently isolated ventriculomegaly.ConclusionsThe prevalence of aneuploidy, congenital infection and neurodevelopmental delay in fetuses with a prenatal diagnosis of isolated unilateral ventriculomegaly is likely to be low. Copyright © 2016 ISUOG. Published by John Wiley & Sons Ltd.
Laparoscopy‐Assisted Proximal Gastrectomy with the Hinged Double Flap Method
Authors: Muraoka Atsushi , Kobayashi Masahiko , Kokudo Yasutaka 
Published: April 2016

AbstractBackgroundNo standard proximal gastrectomy method for gastric cancer of the upper third of the stomach (UGC) has been established because few of the current methods prevent gastro‐esophageal reflux and allow easy postoperative endoscopic surveillance. In the present study, we describe laparoscopic proximal gastrectomy with the hinged double flap method (Kamikawa’s method) in detail and examine the short‐ to medium‐term outcomes of this approach, which resulted in excellent postoperative function.MethodsBetween 2011 and 2015, 24 patients with early‐stage primary UGC underwent laparoscopic proximal gastrectomy with the above‐mentioned method. The celiac and hepatic nerves were preserved without pyloroplasty. A hand‐sewn esophagogastric anastomosis was produced intracorporeally.ResultsThere were no in‐hospital deaths, and none of the patients were converted to open surgery. Complications occurred in two patients in the early part of this study (minor anastomotic leakage in one case and an abdominal abscess in the other). None of the patients exhibited symptoms of reflux. Regarding the patients’ postoperative endoscopic classifications, 17, 2, and 1 patient were considered to have grade N or M, grade A, and grade B esophagitis, respectively. All of the patients remain alive, and no cases of postoperative bowel obstruction or recurrent cancer have been observed.ConclusionsThis method can be performed safely and achieves excellent outcomes in terms of preventing gastro‐esophageal reflux. It deserves further evaluation in a multi‐center clinical study.
Experience of Norwegian Female BRCA1 and BRCA2 Mutation‐Carrying Participants in Educational Support Groups: a Qualitative Study
Authors: Myklebust Marion , Gjengedal Eva , Strømsvik Nina 
Published: April 2016

AbstractWomen identified as BRCA1 and BRCA2 mutation carriers are advised to manage their cancer risk through intensive screening programs and/or by undergoing risk‐reducing surgery. The aim of this study was to explore and describe the experiences of female BRCA1/2 mutation carriers living with cancer risk and their experiences with participation in educational support groups (ESG). This qualitative study enrolled 17 (10 + 7) ESG participants in two different ESGs. The focus group interviews were performed immediately prior to and following two ESGs. The data were analyzed using John Knodel's (1993) practical approach. Three main themes were identified; the women's expectation and experience with ESG, the feeling of loneliness and isolation, and the feelings of living with “something else.” In this paper we have focused on one of the main themes‐ the women's expectation and experience with ESG. This main theme presents four subthemes: the women's need for unambiguous, clear and unified information from health professionals, the need for social support, the important role of the patient representative, and increased knowledge potentially raising concern. Participation in an ESG for women with BRCA1 and BRCA2 mutations can provide relevant information and support in the decision‐making process related to risk‐reducing surgery.
HIV Positive People’s Perspectives on Canadian Criminal Law and Non-Disclosure
Authors: Adam Barry D. , Globerman Jason , Elliott Richard , Corriveau Patrice , English Ken , Rourke Sean 
Published: April 2016

Abstract
The largest survey to date of people living with HIV regarding attitudes toward criminalization of HIV non-disclosure, this study investigates: sources of legal information available to HIV-positive people; perceptions of how criminal prosecutions and media coverage affect understanding of rights and responsibilities of self and others; and where HIV-positive people themselves stand on the role the criminal justice system should play. While mainstream media constructions of criminal iconography do affect PHA views, those who have higher levels of formal education, are active in the dating scene, and have been living longer with HIV hold less punitive views than those who do not. While the overall pattern of agreement on where to draw the line in criminal prosecution holds regardless of demographic characteristics, there is some statistically significant variation in degree of punitiveness according to sexual orientation and gender as well.
Experimental observation of hairpin auto-generation events in a turbulent boundary layer
Authors: Jodai Y. , Elsinga G. E. 
Published: April 2016

Time-resolved tomographic particle image velocimetry experiments show that new hairpin vortices are generated within a fully developed and unperturbed turbulent boundary layer. The measurements are taken at a Reynolds number based on the momentum thickness of 2038, and cover the near-wall region below $y^{+}=140$, where $y^{+}$ is the wall-normal distance in wall units. Instantaneous visualizations of the flow reveal near-wall low-speed streaks with associated quasi-streamwise vortices, retrograde inverted arch vortices, hairpin vortices and hairpin packets. The hairpin heads are observed as close to the wall as $y^{+}=30$. Examples of hairpin packet evolution reveal the development of new hairpin vortices, which are created upstream and close to the wall in a manner consistent with the auto-generation model (Zhou et al., J. Fluid Mech., vol. 387, 1999, pp. 353–396). The development of the new hairpin appears to be initiated by an approaching sweep event, which perturbs the shear layer associated with the initial packet. The shear layer rolls up, thereby forming the new hairpin head. The head subsequently connects to existing streamwise vortices and develops into a hairpin. The time scale associated with the hairpin auto-generation is 20–30 wall units of time. This demonstrates that hairpins can be created over short distances within a developed turbulent boundary layer, implying that they are not simply remnants of the laminar-to-turbulent transition process far upstream.
Vortex dynamics and new lift enhancement mechanism of wing–body interaction in insect forward flight
Authors: Liu Geng , Dong Haibo , Li Chengyu 
Published: April 2016

The effects of wing–body interaction (WBI) on aerodynamic performance and vortex dynamics have been numerically investigated in the forward flight of cicadas. Flapping wing kinematics was reconstructed based on the output of a high-speed camera system. Following the reconstruction of cicada flight, three models, wing–body (WB), body-only (BD) and wings-only (WN), were then developed and evaluated using an immersed-boundary-method-based incompressible Navier–Stokes equations solver. Results have shown that due to WBIs, the WB model had a 18.7 % increase in total lift production compared with the lift generated in both the BD and WN models, and about 65 % of this enhancement was attributed to the body. This resulted from a dramatic improvement of body lift production from 2 % to 11.6 % of the total lift produced by the wing–body system. Further analysis of the associated near-field and far-field vortex structures has shown that this lift enhancement was attributed to the formation of two distinct vortices shed from the thorax and the posterior of the insect, respectively, and their interactions with the flapping wings. Simulations are also used to examine the new lift enhancement mechanism over a range of minimum wing–body distances, reduced frequencies and body inclination angles. This work provides a new physical insight into the understanding of the body-involved lift-enhancement mechanism in insect forward flight.
Extension to nonlinear stability theory of the circular Couette flow
Authors: Yau Pun Wong , Wang Shixiao , Rusak Zvi 
Published: April 2016

A nonlinear stability analysis of the viscous circular Couette flow to axisymmetric finite-amplitude perturbations under axial periodic boundary conditions is developed. The analysis is based on investigating the properties of a reduced Arnol’d energy-Casimir function $\mathscr{A}_{rd}$ of Wang (Phys. Fluids, vol. 2, 2009, 084104). A weighted kinetic energy of the perturbation, which has a form of ${\rm\Delta}\mathscr{A}_{rd}$, the difference between the reduced Arnol’d function and its base flow value, is used as a Lyapunov function. We show that all the inviscid flow effects as well as all the viscous-dependent terms that are related to the flow boundaries vanish. The evolution of ${\rm\Delta}\mathscr{A}_{rd}$ depends only on the viscous effects of the perturbation’s dynamics inside the flow domain. The requirement for the temporal decay of ${\rm\Delta}\mathscr{A}_{rd}$ leads to two novel sufficient conditions for the nonlinear stability of the circular Couette flow in response to axisymmetric perturbations. The linearized version of these conditions for infinitesimally small perturbations recovers the recent linear stability results by Kloosterziel (J. Fluid Mech., vol. 652, 2010, pp. 171–193). By examining the nonlinear stability conditions, we establish a definite operational region of the viscous circular Couette flow that is independent of the fluid viscosity. In this region of operation, the flow is nonlinearly stable in response to perturbations of any size, provided that the initial total circulation function is above a minimum level determined by the operational conditions of the base flow. Comparisons with historical studies show that our results shed light on the experimental measurements of Wendt (Ing.-Arch., vol. 4, 1933, pp. 577–595) and extend the classical nonlinear stability results of Serrin (Arch. Rat. Mech. Anal., vol. 3, 1959, pp. 1–13) and Joseph & Hung (Arch. Rat. Mech. Anal., vol. 44, 1971, pp. 1–22). When the flow is nonlinearly stable and evolves axisymmetrically for all time, then it always decays asymptotically in time to the circular Couette flow determined uniquely by the set-up of the rotating cylinders. Finally, we derive upper-bound estimates on the decay rate of finite-amplitude perturbations for the solid-body rotation flow between two coaxial rotating cylinders and for the circular Couette flow. We demonstrate via numerical simulations that the theoretical upper bound is relevant to the dynamics of various axisymmetric perturbations tested, where it is strictly obeyed. This present study provides new physical insights into a classical flow problem that was studied for many decades.
The Reception of E.P. Thompson in Japan: The New Left, The Making, and “Moral Economy”
Authors: Ichihashi Hideo 
Published: April 2016

AbstractThis article traces the chequered history of the reception of E.P. Thompson in postwar Japan and tries to assess what kind of impact his thoughts and ideas had on the Japanese intellectual world. In so doing, this article will draw on interviews with several academics in Japan from various generations as well as written documents. The article begins with a survey of postwar left-wing politics in Japan, against which background Thompson was introduced as a New Left thinker. It also considers the National History Movement, whose problematic legacy seemed to condition the reception of The Making of the English Working Class in Japan in the 1960s. After exploring the limited reception of The Making among Japanese historians, we witness the more favourable reception of the concept of “moral economy”. The article demonstrates that the rather awkward history of the reception of E.P. Thompson in Japan cannot be understood without referring to the postwar concerns of Japanese intellectuals, concerns that changed fairly dramatically in the course of time.
From Structuralism to Culturalism: The Protracted German Reception ofThe Making of the English Working Classand its Actuality Reassessed from a Post-Cold War Perspective
Authors: Lindenberger Thomas 
Published: April 2016

AbstractBased on the author’s experience as one of the German translators ofThe Making, this article lays out its protracted and contradictory reception in Germany. When E.P. Thompson’smagnum opuswas published fifty years ago, German scholars on both sides of the Iron Curtain failed to take note of it for several years. The relatively muted reception in West Germany during the 1970s was marked by its dismissal as theory-lacking and “subjectivist”. Examining the contrasting contexts of postwar Britain, with its popular anti-fascist experience, and post-fascist West Germany helps to understand why Thompson’s “empirical idiom” of class history failed to strike a chord at the time with leading representatives of the new generation of “progressive” social historians in Germany and a broader reading public. It was only with the arrival ofAlltagsgeschichte, feminist history, and, more generally, the cultural turn in humanities thatThe Makingand its German translation became a canonical point of reference both in working-class history and the wider humanities. A brief epilogue discusses its lasting potential for a historical understanding of today’s processes of post-Cold War class formation and human rights struggles.
South African History and Subaltern Historiography: Ideas for a Radical History of White Folk
Authors: Roos Neil 
Published: April 2016

AbstractIn considering how “radical” histories of ordinary whites under apartheid might be written, this essay engages with several traditions of historical scholarship “from” and “of” below. For three decades, Marxist-inspired social history dominated radical historiography in South Africa. It has, however, proved little able to nurture historiography of whites that is politically engaged and acknowledges post-Marxist currents in the discipline. I advocate a return to theory and suggest that new sources may be drawn from the academy and beyond. Historiographies “of” below need not necessarily be historiographies “from” below and this article proposes the idea of a “racial state” as an alternative starting point for a history of apartheid-era whites. It goes on to argue that Subaltern Studies, as a dissident, theoretically eclectic and interdisciplinary current in historiography offers useful perspectives for exploring the everyday lives of whites in South Africa. After suggesting a research agenda stemming from these theoretical and comparative insights, I conclude by reflecting on the ethics of writing histories of apartheid-era whites.
The timescales of global surface-ocean connectivity
Authors: Jönsson Bror F. , Watson James R. 
Published: April 2016

AbstractPlanktonic communities are shaped through a balance of local evolutionary adaptation and ecological succession driven in large part by migration. The timescales over which these processes operate are still largely unresolved. Here we use Lagrangian particle tracking and network theory to quantify the timescale over which surface currents connect different regions of the global ocean. We find that the fastest path between two patches—each randomly located anywhere in the surface ocean—is, on average, less than a decade. These results suggest that marine planktonic communities may keep pace with climate change—increasing temperatures, ocean acidification and changes in stratification over decadal timescales—through the advection of resilient types.
Supramolecular macrocycles reversibly assembled by Te…O chalcogen bonding
Authors: Ho Peter C. , Szydlowski Patrick , Sinclair Jocelyn , Elder Philip J. W. , Kübel Joachim , Gendy Chris , Lee Lucia Myongwon , Jenkins Hilary , Britten James F. , Morim Derek R. , Vargas-Baca Ignacio 
Published: April 2016

AbstractOrganic molecules with heavy main-group elements frequently form supramolecular links to electron-rich centres. One particular case of such interactions is halogen bonding. Most studies of this phenomenon have been concerned with either dimers or infinitely extended structures (polymers and lattices) but well-defined cyclic structures remain elusive. Here we present oligomeric aggregates of heterocycles that are linked by chalcogen-centered interactions and behave as genuine macrocyclic species. The molecules of 3-methyl-5-phenyl-1,2-tellurazole 2-oxide assemble a variety of supramolecular aggregates that includes cyclic tetramers and hexamers, as well as a helical polymer. In all these aggregates, the building blocks are connected by Te…O–N bridges. Nuclear magnetic resonance spectroscopic experiments demonstrate that the two types of annular aggregates are persistent in solution. These self-assembled structures form coordination complexes with transition-metal ions, act as fullerene receptors and host small molecules in a crystal.