bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomes of Candidatus Wolbachia bourtzisii wDacA and Candidatus Wolbachia pipientis wDacB from the Cochineal Insect Dactylopius coccus (Hemiptera: Dactylopiidae)
Authors: Ramírez-Puebla Shamayim T , Ormeño-Orrillo Ernesto , Vera-Ponce de León Arturo , Lozano Luis , Sanchez-Flores Alejandro , Rosenblueth Mónica , Martínez-Romero Esperanza 
Published: August 2016

Abstract
Dactylopius species, known as cochineal insects, are the source of the carminic acid dye used worldwide. The presence of two Wolbachia strains in Dactylopius coccus from Mexico was revealed by PCR amplification of wsp and sequencing of 16S rRNA genes. A metagenome analysis recovered the genome sequences of Candidatus Wolbachia bourtzisii wDacA (supergroup A) and Candidatus Wolbachia pipientis wDacB (supergroup B). Genome read coverage, as well as 16S rRNA clone sequencing, revealed that wDacB was more abundant than wDacA. The strains shared similar predicted metabolic capabilities that are common to Wolbachia, including riboflavin, ubiquinone, and heme biosynthesis, but lacked other vitamin and cofactor biosynthesis as well as glycolysis, the oxidative pentose phosphate pathway, and sugar uptake systems. A complete tricarboxylic acid cycle and gluconeogenesis were predicted as well as limited amino acid biosynthesis. Uptake and catabolism of proline were evidenced in Dactylopius Wolbachia strains. Both strains possessed WO-like phage regions and type I and type IV secretion systems. Several efflux systems found suggested the existence of metal toxicity within their host. Besides already described putative virulence factors like ankyrin domain proteins, VlrC homologs, and patatin-like proteins, putative novel virulence factors related to those found in intracellular pathogens like Legionella and Mycobacterium are highlighted for the first time in Wolbachia. Candidate genes identified in other Wolbachia that are likely involved in cytoplasmic incompatibility were found in wDacB but not in wDacA.
RNAi-Based Suppressor Screens Reveal Genetic Interactions Between the CRL2LRR-1 E3-Ligase and the DNA Replication Machinery inCaenorhabditis elegans
Authors: Ossareh-Nazari Batool , Katsiarimpa Anthi , Merlet Jorge , Pintard Lionel 
Published: August 2016

AbstractCullin-RING E3-Ligases (CRLs), the largest family of E3 ubiquitin-Ligases, regulate diverse cellular processes by promoting ubiquitination of target proteins. The evolutionarily conserved Leucine Rich Repeat protein 1 (LRR-1) is a substrate-recognition subunit of a CRL2LRR-1 E3-ligase. Here we provide genetic evidence supporting a role of this E3-enzyme in the maintenance of DNA replication integrity in Caenorhabditis elegans. Through RNAi-based suppressor screens of lrr-1(0) and cul-2(or209ts) mutants, we identified two genes encoding components of the GINS complex, which is part of the Cdc45-MCM-GINS (CMG) replicative helicase, as well as CDC-7 and MUS-101, which drives the assembly of the CMG helicase during DNA replication. In addition, we identified the core components of the ATR/ATL-1 DNA replication checkpoint pathway (MUS-101, ATL-1, CLSP-1, CHK-1). These results suggest that the CRL2LRR-1 E3-ligase acts to modify or degrade factor(s) that would otherwise misregulate the replisome, eventually leading to the activation of the DNA replication checkpoint.
Production of Small Noncoding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster
Authors: Guida Vincenzo , Cernilogar Filippo M , Filograna Angela , De Gregorio Roberto , Ishizu Hirotsugu , Siomi Mikiko C , Schotta Gunnar , Bellenchi Gian Carlo , Andrenacci Davide 
Published: August 2016

Abstract
Protective mechanisms based on RNA silencing directed against the propagation of transposable elements are highly conserved in eukaryotes. The control of transposable elements is mediated by small noncoding RNAs, which derive from transposon-rich heterochromatic regions that function as small RNA-generating loci. These clusters are transcribed and the precursor transcripts are processed to generate Piwi-interacting RNAs (piRNAs) and endogenous small interfering RNAs (endo-siRNAs), which silence transposable elements in gonads and somatic tissues. The flamenco locus is a Drosophila melanogaster small RNA cluster that controls gypsy and other transposable elements, and has played an important role in understanding how small noncoding RNAs repress transposable elements. In this study, we describe a cosuppression mechanism triggered by new euchromatic gypsy insertions in genetic backgrounds carrying flamenco alleles defective in gypsy suppression. We found that the silencing of gypsy is accompanied by the silencing of other transposons regulated by flamenco, and of specific flamenco sequences from which small RNAs against gypsy originate. This cosuppression mechanism seems to depend on a post-transcriptional regulation that involves both endo-siRNA and piRNA pathways and is associated with the occurrence of developmental defects. In conclusion, we propose that new gypsy euchromatic insertions trigger a post-transcriptional silencing of gypsy sense and antisense sequences, which modifies the flamenco activity. This cosuppression mechanism interferes with some developmental processes, presumably by influencing the expression of specific genes.
Das Geschlecht der Staatenlosen. Staatenlosigkeit in der BundesrepublikDeutschland nach 1945
Authors: Rürup Miriam 
Published: August 2016

What's the Sex of the Stateless? Statelessness in Post-war West-Germany The stateless person is a central figure of the twentieth century. It developed as a result of the confluence of nationalism, the emergence of new nation states, the dissolution of former empires and the two World Wars. Of all the major causes of statelessness, one had particularly affected women. Even long into the twentieth century – and in international law, until 1957 – a woman's nationality was based on the principle of «derivative citizenship». This meant that her citizenship depended on the person to whom she «belonged», be it her father in her country of origin or her husband of a different nationality. This article focuses on international judicial, diplomatic and humanitarian discussions in the preliminary stages of resolutions such as the 1957 UN Convention on the Nationality of Married Women. The discussions on the supranational level were echoed by the West-German claim of equality of the sexes, which was laid out in national legislation such as the Basic Law. By looking at this example, we can find out how both the nation states as well as women's rights groups acted as players in an international field of diplomatic discourse, and how they employed a human rights language in order to make themselves heard and to convey their claims. While the «right to have a nationality» was part of the 1948 Declaration of Human Rights, the question of how to deal with stateless people on an everyday basis of political and social practice within the framework of the nation state still remained unanswered. My interest is to show how women's rights groups and German national legislation discovered and defined statelessness as a humanitarian problem and tried to solve it in the political realm.
El virus del Zika, sus consecuencias en la calidad de vida de la población y en el sistema de salud y seguridad social de Colombia
Authors: Pérez Cárdenas Jorge Enrique 
Published: August 2016

Un artículo reciente de la revista New England Journal of Medicine (1), confirmó que el virus del Zika es el productor de microcefalia en niños. La asociación directa entre el virus del Zika y el sistema nervioso central era lo que faltaba para respaldar esta aseveración.
El reporte fue realizado en una mujer embarazada de origen europeo que trabajó en una zona con presencia del Zika en Brasil. La paciente presentó las manifestaciones clínicas de la enfermedad al finalizar el primer trimestre de embarazo. Posteriormente viajó a Europa, donde mediante examen ecográfico del feto encontraron retardo en el desarrollo y microcefalia. Por indicación médica y con el consentimiento de la madre se decidió practicar la terminación del embarazo.
Fenomenología del daño: el “mal aire” y los rasgos del no-mundo para los habitantes de el placer
Authors: Botero Ángela Uribe 
Published: August 2016

El propósito de este artículo es mostrar cómo ciertos procesos de victimización condenan a quienes los padecen a una suerte de destierro del mundo y de la realidad. El caso de la larga presencia paramilitar en El Placer (Putumayo, Colombia) es un ejemplo de lo anterior. Con el uso que hacen los sobrevivientes en El Placer de los términos “el mal aire” y “los aires de la muerte” se expresan nítidamente dichas formas de destierro. El propósito general del texto se cumple, en primer lugar, recurriendo al término ‘mundo’ tal como este es usado por Hannah Arendt y por Edmund Husserl y, en segundo lugar, al término ‘realidad’ propuesto por Arendt y por Somogy Varga. Para concluir, intento establecer una relación entre la pérdida de mundo, la pérdida del sentido de la realidad y lo que se conoce en fenomenología como‘despersonalización’. AbstractThe purpose of this article is to show how certain forms of victimization condemn those who suffer them to a sort of exile from the world and from reality. The case of the long paramilitary presence in the town El Placer (Putumayo, Colombia) is a good example of this. The use its survivors make of the words “bad air” and “the airs of death” neatly expresses the mentioned forms of exile. To accomplish this purpose, firstly, I appeal, to the term ‘world’ as used by Hannah Arendt and Edmund Husserl, and secondly, to the term ‘reality’ as usedby Arendt and Somogy Varga. I conclude weaving a relationship between the terms “no-world”, “loss of the sense of reality”and what in phenomenology is known as ‘depersonalization’.
Synovial Chondromatosis of the Knee in a 2-Year-Old Child
Authors: Temponi Eduardo Frois , Mortati Rafael Borghi , Mortati Giselle Mayumi Hayashi , Mortati Lucas Borghi , Sonnery-Cottet Bertrand , de Carvalho Júnior Lúcio Honório 
Published: August 2016

Case:
We describe a rare case of synovial chondromatosis of the knee of a 2-year-old child. The diagnosis was based on the history, physical examination, and complementary examinations (radiography and magnetic resonance imaging). Anterior and posterior approaches were used for total synovectomy and resection of loose bodies.

Conclusion:
Physicians should keep this condition in mind, even in young children, because early identification prevents future secondary degenerative changes in the knee joint. As far as we know, this is the youngest child with synovial chondromatosis of the knee reported in the English-language literature.
MINERALOGICAL CHARACTERIZATION AND CHEMICAL PROPERTIES OF SOILS AS A CONSIDERATION FOR ESTABLISHING SUSTAINABLE SOIL MANAGEMENT STRATEGIES
Authors: Anda Markus , Mulyani Anny , Suparto Suparto 
Published: August 2016

Problems of soil limiting factors affecting crop growth were not fully understood owing to little attention has been given to soil mineralogy and its association to soil chemical properties. The objective of the study was to assess soil mineralogical and chemical properties of seven soils derived from different parent<br />materials as an integrated strategic consideration to establish soil management. Field study was carried out in 2009. Soils were sampled from each horizon of profiles. Results showed that primary and secondary minerals had a strong effect on soil chemical properties. The sand fraction of soils derived from basalt, gabbro, mica schist and serpentinite was dominated by resistant minerals (quartz or opaques), leading to very limited, if any, nutrients released from parent materials. The clay fraction was dominated by kaolinite for soils derived from<br />basalt, mica schist and gabbaro, and by amorphous materials for the soil derived from serpentinite resulting in low soil cation exchange capacity (CEC). For other three soils derived from volcanic tuff, volcanic basaltic andesite and volcanic ash, the easily weatherable minerals (feldspar and ferromagnesian) were dominant, suggesting the high reserved nutrients. The presence of halloysite and smectite minerals in the soil derived from volcanic tuff resulted in high soil CEC, while the dominance of amorphous materials in soils derived from volcanic basaltic andesite and volcanic ash was responsible for the low CEC. For<br />soils derived from basalt, serpentinite, mica schist and gabbro, therefore, the strategic management should be directed to restore soil CEC, pH, exchangeable cations and P content. For soils derived from volcanic tuff, volcanic basaltic andesite and volcanic ash, the presence of easily weatherable minerals indicated many reserved nutrients; hence the soil management is directed for replenishment of nutrients removed by crops. <br />
EFFECTS OF LATE BLIGHT RESISTANT POTATO CONTAINING RB GENE ON THE SOIL MICROBES, PESTS AND PLANT DISEASES
Authors: Riyanti Eny Ida , Listanto Edy , Ambarwati Alberta Dinar 
Published: August 2016

Late blight caused by Phytophthora infestans is an important disease on potato. Several potato hybrids have been generated by crossing local varieties (Atlantic and Granola) with Katahdin SP951 which contains late blight resistance gene RB. Prior to release, these hybrids need to be evaluated for their environ-mental effects on non-target organisms and natural pests and diseases. The objectives of the study were to investigate the effect of LBR potato hybrids on beneficial soil microbes, pests and diseases. The trial was conducted in the confined field trial (CFT) in Lembang, West Java. The parental non-transgenic (NT) clones (Granola, Atlantic and Katahdin) and LBR hybrids (four clones of Atlantic x Katahdin SP951 hybrids; 10 clones of Granola x Katahdin SP951) were planted at a plant spacing of 30 cm x 70 cm. Fungicide applications were used as treat-ments (no spray, five and twenty times sprays). The experi-ment was arranged in a randomized completely block design with three replications. The parameters determined were popula-tions of N2 fixing and P solubilizing bacteria, soil C/N ratio as well as natural pests and diseases. The results showed that the transgenic LBR potato hybrids did not have negative effect on N fixing bacteria. The bacterial populations were around 1010-11 cells g-1 soil before planting, 1012 cells at 1.5 months after planting (MAP) and 108 cells after harvest. For P- solubilizing bacteria, their populations were 1010 cells before planting, 1012 cells at 1.5 MAP and 1011 cells g-1 soil after harvest. The soil C/N ratio of the transgenic plot was not statistically different compared to non-transgenic plot, i.e. 12-15 before planting, 10-11 at 1.5 MAP, and 10 after harvest in non-spray plot. Pests and diseases such as Alternaria solani, Liriomyza, potato tubber moth, aphid and mites on the transgenic and non-transgenic plots were statistically not different. The resistance score for A. solani was 7.2 (parental tansgenic) and 7.6 (parental non-transgenic); for Liriomyza it was 2.07 (parental transgenic) and 2.32 insect per plant (parental non-transgenic), the PTM was 0.63 (parental transgenic) and 0.73 insect per plant (parental non-transgenic), aphid and mites were 0.75 (parental transgenic) and 1.68 insects per plant (parental non-transgenic). The study indicated that LBR potato hybrids did not have any negative impacts on non-target organisms.
Quality function and the quality manager
Authors: Hani Dr.Sc. Miraç 
Published: August 2016

In many organizations, management systems are viewed in terms of the internal dynamics between marketing, design, production, distribution, and accounting. A change is required from this to a larger system which also encompasses and integrates the business interests of customers and suppliers. Management needs to develop an in-depth understanding of these relationships and how they may be used to cement the partnership concept. The quality function should be the organization’s focal point in this respect and should be equipped to gauge internal and external customers, expectations and degree of satisfaction. It should also identify quality deficiencies in all business functions and promote improvements.The role of the quality function is to make quality become an inseparable aspect of every employee’s performance and responsibility. The transition in many companies from quality departments with line functions will require careful planning, direction and monitoring. Quality professionals have developed numerous techniques and skills focused on product or service quality.
The Involvement of Albanian Ethnic Immigrants in Entrepreneurship as a New Possibility on their Integration in the International Labour Market - Their Role in Transnational Bridging For Homemade Products (Case Of The Province Of Milan, Italy)
Authors: Bejta MA. Ilir , Fetahu MSc. Elvira 
Published: August 2016

It is like a circle, using business to build social integration and using social integration to increase competitiveness in the labour market for the Albanian ethnic immigrant minorities, increasing as well their human capital capacities, especially bridging transnational on domestic products and brands in order to make it easier for those to internationalize. Consequently, it will valorise their social position in the societies they live and work.This paper addresses and analysis, as well as evaluates the role entrepreneurship has on the social development and social integration of the ethnic immigrant minorities and the increase of their attractiveness in the international labour market.The paper addresses also the reasons and factors impacting the emerging of ethnic immigrant entrepreneurship worldwide and in EU. It analyzes their role on the world entrepreneurship and economic system, the actual situation of ethnic immigrant minorities, their social networks and organizations emerged due to their role as a new force and human capital in these markets. In this framework, being a transnational bridge on the internationalization of their home country products and culture, the Albanian ethnic immigrant entrepreneurship, as the main focus group of this study, can increase its possibilities to be more competitive in the labour market.Economic integration induces immigrants to increase contacts and be part of economic networks and social networks too, as well as improve, as a necessity deriving from the economic competition, their human capital capacities and capabilities getting use of international labour market. Considering economic and human capital development we will measure their impact on the social welfare and integration of the ethnic immigrant minority groups in the modern society (Albanians in Province of Milan, Italy).
The Inevitability of Strengthening the Nation-Building Subjectivity of Ethnic and National Groups
Authors: Hadzic Dr.sc. Harun 
Published: August 2016

This paper presents a very courageous, scientifically founded prediction, concerning the imminent and inevitable process of strengthening the political and nation-building subjectivity of national minorities and ethnic groups, i.e., minority indigenous people. For, a more lasting peace and stability, for which we want to dominate in Europe and the world, can only be achieved by deflection of the discontent of these nationalities, on one hand, and strengthening of the public recognition of their international legal subjectivity, on the other hand. This implies the exercise of their right to self-determination and the creation of independent autonomies, regions or countries, which also implies reclassification and conversion of the internal, but also of the interstate borders. These new limits must be established precisely in those parts of the world where there is a political struggle, and a long-time demand of these organized groups, which would, in effect, mean that the international community should accept their demand for creation of new states, or, at least, another, lower, level of independence.
Calculation of Investments for the Distribution of GPON Technology in the village of Bishtazhin through database
Authors: Qarkaxhija MSc. Jusuf , Luarasi Dr.Sc. Tamara 
Published: August 2016

According to daily reports, the income from internet services is getting lower each year. Landline phone services are running at a loss, whereas mobile phone services are getting too mainstream and the only bright spot holding together cable operators (ISP) in positive balance is the income from broadband services (Fast internet, IPTV).Broadband technology is a term that defines multiple methods of information distribution through internet at great speed. Some of the broadband technologies are: optic fiber, coaxial cable, DSL, Wireless, mobile broadband, and satellite connection. The ultimate goal of any broadband service provider is being able to provide voice, data and the video through a single network, called triple play service.The Internet distribution remains an important issue in Kosovo and particularly in rural zones.Considering the immense development of the technologies and different alternatives that we can face, the goal of this paper is to emphasize the necessity of a forecasting of such investment and to give an experience in this aspect. Because of the fact that in this investment are involved many factors related to population, geographical factors, several technologies and the fact that these factors are in continuously change, the best way is, to store all the data in a database and to use this database for different results. This database helps us to substitute the previous manual calculations with an automatic procedure of calculations.This way of work will improve the work style, having now all the tools to take the right decision about an Internet investment considering all the aspects of this investment.
Study of Organochlorinated Pesticide Residues and Polychlorinated Biphenyls in Soil Samples
Authors: Gashi MSc. Vlora , Nuro Dr.Sc. Aurel , Pulaj MSc. Basri 
Published: August 2016

This paper presents and discusses the data obtained for organochlorinated pesticides and their residues in the soil samples of agricultural areas. Soil contamination is one of most important factors influencing the quality of agricultural products. Usage of heavy farm equipment, the land drainage, an exces­sive application of agrochemicals, emissions originating from mining, metallurgical, and chemical and coal power plants and transport, all generate a number of undesired substances (nitric and sulphur oxides, PAHs, heavy metals, pesticides), which after deposition in soil may influence crop quality. Thus, input of these contaminants into the environment should be carefully monitored. Levels of organochlorinated pesticides contamination were evaluated in agriculture areas that are in use. 10 soil samples were taken in agricultural areas Plane of Dugagjini , Kosovo. Representa­tive soil samples were collected from 0-30 cm top layer of the soil.In the analytical method we combined ultrasonic bath extraction and a Florisil column for samples clean-up. The analysis of the organochlorinated pesticides in soil samples was performed by gas chromatography technique using electron capture detector (GC/ECD). Optima-5 (low/mid polarity, 5% phenyl methyl siloxane 60 m x 0.33 mm x 0.25μm film) capillary column was used for isolation and determination of organochlorinated pesticides. Low concentrations of organochlorinated pesticide and their metabolites were found in the studied samples. The presence of organochlorinated pesticides and their residues is probably resulting of their previous uses for agricultural purposes.