bluuebunny/crossref_metadata_2025_split huggingface dataset:
Schlankheitskur für Stützen in einer Bonner Tiefgarage
Authors: Walz Marcus , Bach Alexander , Scherzer Jan , Loos Julian 
Published: September 2024

AbstractUnter der Bundesstraße 56 in Bonn befindet sich eine Tiefgarage, die sich über drei Untergeschosse erstreckt und 425 Parkplätze bietet. Die Zugangsmöglichkeiten sind über die Straße Annagraben und eine Zufahrtsrampe unter dem Sterntorhaus. Über der Tiefgarage verläuft eine Straßenbahntrasse. Die Tiefgarage, errichtet in den 1960er‐Jahren, trägt Lasten aus Schwerverkehr und Straßenbahn durch massive Stützen in den Untergrund ab. Aufgrund der starken Kontaminierung des Betons durch Chloride, insbesondere im Bereich der Stützen, ist eine umfangreiche Instandsetzung der Tiefgarage erforderlich. Zudem entspricht die Betondeckung nicht mehr dem Stand der Technik und ist v. a. an den Stützen deutlich zu gering. Da die Stellplatzbreiten mit 2,20 m sehr nutzerunfreundlich sind, ist eine Instandsetzung durch Erhöhung der Betondeckung nicht möglich. Aus diesem Grund hat man sich dazu entschlossen, die betreffenden Stützen, insgesamt 154 Stück, vollständig zu entfernen und anschließend wieder neu mit geringeren Abmessungen zu erstellen. Die Nachrechnung der obersten Stahlbetondecke erfolgte nach der „Richtlinie zur Nachrechnung von Straßenbrücken im Bestand“. Verkehrslast und Erdüberschüttung wurden präzise vermessen. Die ausreichende Tragfähigkeit der Decke wurde nachgewiesen. Neue Stützen werden stumpf angeschlossen und nicht monolithisch mit den bestehenden Decken verbunden, was eine Neuberechnung der Stahlbetonkonstruktion erfordert. Eine Fertigteillösung wurde wegen Einbringungsproblemen verworfen. Aufgrund der Brandschutzanforderungen wurde eine Stahlbetonlösung bevorzugt. Die Untersuchung zeigt, dass die vorgeschlagene Lösung tragfähig und brandschutzkonform ist.
Association Between Self‐Reported Infections and Seropositivity Among Pregnant Women With Gastroschisis: A Case Control Study, With Emphasis on Chlamydia trachomatis
Authors: Feldkamp M. L. , Baum‐Jones E. , Enioutina E. Y. , Krikov S. , Kamath K. 
Published: September 2024

ABSTRACTBackgroundGastroschisis is a birth defect with the greatest risk among women <20 years of age.MethodsPregnant women attending the University of Utah's Maternal‐Fetal Medicine Diagnostic Center between 2011 and 2017 for either their routine diagnostic ultrasound or referral were recruited (cases: pregnant women with fetal gastroschisis, n = 53 participated/57, 93%; controls: pregnant women without fetal abnormalities, n = 102 participated/120, 85%). A clinic coordinator consented and interviewed women and obtained a blood sample and prenatal medical records. We evaluated self‐reported maternal characteristics, risk factors, and infections. To assess pathogen seropositivity we used Serimmune's Serum Epitope Repertoire Analysis validated 35 pathogen panels and Chlamydia trachomatis and compared seropositivity to self‐report and prenatal medical record screening to assess sensitivity.ResultsCases were more likely to report a younger age at sexual debut (p = <0.01), more sexual partners (p = 0.02), being unmarried (p < 0.01), changing partners between pregnancies (p = <0.01), smoking cigarettes (<0.01), and a recent sexually transmitted infection (STI) (p = 0.02). No differences were observed for self‐report of illicit drug use or periconceptional urinary tract infections. Cases had a higher seropositivity for cytomegalovirus (p = 0.01). No differences were observed for herpes simplex I, II, or Epstein–Barr. Though based on small numbers, C. trachomatis seropositivity was highest in cases (17%) compared to controls (8.8%) with the highest proportion observed in case women <20 years of age (cases 33%; controls 0%). Any STI (self‐report or seropositivity) was also highest among cases <20 years of age (cases 47%; controls 0%). Among C. trachomatis seropositive women, self‐report and prenatal medical record sensitivity was 27.8% and 3%, respectively.ConclusionsCases were more likely to engage in behaviors that can increase their risk of exposure to sexually transmitted pathogens. Case women <20 years of age had the highest proportion of C. trachomatis seropositivity and any STI. Prenatal medical records and self‐report were inadequate to identify a recent chlamydial infection whereas, the SERA assay is a novel approach for evaluating subclinical infections that may impact the developing embryo.
A highly specific red‐emitting fluorescent probe for monitoring of Cu2+ in living cells and zebrafish
Authors: Kong Shuai , Zhang Yan , Xie Lianke , Qi Guodong , Li Yanping , Dai Haitao , Qi Qiubo , Chen Yimin 
Published: September 2024

AbstractA novel seminaphthorhodafluor‐based fluorescent probe was designed and prepared. It exhibited an outstanding off–on fluorescence response (about 45‐fold enhancement) to Cu2+ because the structure changed from a spirocyclic form to a ring‐open state. It showed satisfactory detection performances for Cu2+ with fast response speed, high selectivity and anti‐interference, and a low detection limit (22.9 nM) due to its long emission wavelength and off–on fluorescence response mode. This probe was also satisfactorily applied for the monitoring of Cu2+ in living cells and zebrafish.
Development of a multigene expression system using 2A peptides in Rhodosporidium toruloides
Authors: Guo Xiao , Bai Zhenzhen , Zhao Huimin , Shi Shuobo 
Published: September 2024

AbstractIn eukaryotes, gene expression typically requires individual promoter and terminator for each gene, making the expression of multiple genes tedious and sometimes too difficult to handle. This is especially true for underdeveloped nonmodel organisms with few genetic engineering tools and genetic elements such as Rhodosporidium toruloides. In contrast, polycistronic expression offers advantages such as smaller size and ease of cloning. Here we report the development of a multigene expression system using 2A peptides in R. toruloides. First, twenty‐two 2A peptides were evaluated for their cleavage efficiencies, which ranged from 33.65% to 93.32%. Subsequently, the 2A peptide of ERBV‐1 with the highest efficiency was selected to enable simultaneous expression of four proteins. In addition, we demonstrated the optimization of the α‐linolenic acid biosynthetic pathway using ERBV‐1 peptide mediated polycistronic expression, which increased the α‐linolenic acid production by 104.72%. These results suggest that using ERBV‐1 peptide is an efficient strategy for multigene expression in R. toruloides.
Exploring the power of informal institutions: How does social trust affect corporate's greenwashing strategy
Authors: Shuang Qi , Lu Jintao , Wang Sen , Callari Denise , Cucari Nicola 
Published: September 2024

AbstractCorporate greenwashing seriously impedes global sustainable development. Despite the discussion on the factors influencing corporate greenwashing strategy, the impact of informal institutions, particularly social trust, remains underexplored. Based on institutional theory and the attention‐based view, this study examines the effect of social trust on corporate greenwashing strategy. Additionally, it also explores the mediating roles of managers' environmental attention and firms' green open innovation, as well as the moderating roles of government environmental regulation and market competition. Using a sample of 3,143 heavily polluting companies listed on China's A‐share market, the results show that social trust negatively impacts corporate greenwashing strategy. Managers' environmental attention and firms' green open innovation are the chain mediators through which social trust constrains firms' greenwashing strategy. Moreover, government environmental regulation strengthens the effect of social trust on corporate greenwashing, while market competition weakens the effect. The obtained conclusions provide an important guidance for curbing corporate greenwashing strategy and highlight the role of social trust.
Fed‐batch strategies for intensified rVSV vector production in high cell density cultures of suspension HEK293 cells
Authors: Silva Cristina A. T. , Kamen Amine A. , Henry Olivier 
Published: September 2024

AbstractVesicular stomatitis virus (VSV) has been increasingly demonstrated as a promising viral vector platform. As the interest over this modality for vaccine and gene therapy applications increases, the need for intensified processes to produce these vectors emerge. In this study, we develop fed‐batch‐based operations to intensify the production of a recombinant VSV‐based vaccine candidate (rVSV‐SARS‐CoV‐2) in suspension cultures of HEK293 cells. A feeding strategy, in which a commercial concentrated medium was added to cultures based on cell growth through a fixed cell specific feeding rate (CSFR), was applied for the development of two different processes using Ambr250 modular bioreactors. Cultures operated in hybrid fed‐batch/perfusion (FB/P) or fed‐batch (FB) were able to sustain infections performed at 8.0 × 106 cells/mL, respectively resulting in 3.9 and 5.0‐fold increase in total yield (YT) and 1.7 and 5.6‐fold increase in volumetric productivity (VP) when compared with a batch reference. A maximum viral titer of 4.5 × 1010 TCID50/mL was reached, which is comparable or higher than other processes for VSV production in different cell lines. Overall, our study reports efficient fed‐batch options to intensify the production of a rVSV‐based vaccine candidate in suspension HEK293 cells.
MRSA Inhibitory Activity of Some New Pyrazolo[1,5‐a]pyrimidines Linked to Arene and/or Furan or Thiophene Units
Authors: Elneairy Mohamed A. A. , Youssef Emad G. N. , Ebrahim Sama A. A. , Mohammad Nour E. M. , Abd El‐Rahman Nourhan M. S. , Elhewaty Ahmed S. M. , Sanad Sherif M. H. , Mekky Ahmed E. M. 
Published: September 2024

AbstractMethicillin‐resistant Staphylococcus aureus (MRSA) is a major contributor to hospital‐acquired infections and is highly resistant to treatment. Ongoing research focuses on developing new antimicrobial medications to prevent the spread of resistance. A facile method was employed to efficiently synthesize new pyrazolo[1,5‐a]pyrimidines in 84–93 % yields by reacting 4‐benzyl‐1H‐pyrazole‐3,5‐diamine with the respective α,β‐unsaturated ketones. The reaction was carried out in ethanol containing 1.2 equivalents of potassium hydroxide at reflux for 5–6 h. The new products are attached to a para‐substituted aryl group with variable electronic properties at pyrazolopyrimidine‐C5, in addition to one of three units at C7, namely phenyl, thiophen‐2‐yl, or furan‐2‐yl units. A wide spectrum of antibacterial activity was displayed by the new pyrimidines against six different bacterial strains. In general, pyrimidines attached to furan‐2‐yl units at C7, in addition to another aryl unit at C5, attached to 4‐Me or 4‐OMe groups, demonstrate significant antibacterial activity, particularly against S. aureus strain. They had MIC/MBC of 2.5/5.1 and 2.4/4.9 μM, respectively, which exceeded that of ciprofloxacin. Moreover, they demonstrate more effective MRSA inhibitory activity than linezolid, with MIC/MBC values up to 4.9/19.7 and 2.4/19.7 μM against MRSA ATCC:33591 and ATCC:43300 strains, respectively.
Ectonucleotidase activity driven by acid ectophosphatase in luminal A MCF‐7 breast cancer cells
Authors: Lacerda‐Abreu Marco Antonio , Mendonça Bruna dos Santos , Nestal de Moraes Gabriela , Meyer‐Fernandes José Roberto 
Published: September 2024

AbstractEctophosphatases catalyse the hydrolysis of phosphorylated molecules, such as phospho‐amino acids, in the extracellular environment. Nevertheless, the hydrolysis of nucleotides in the extracellular environment is typically catalysed by ectonucleotidases. Studies have shown that acid ectophosphatase, or transmembrane‐prostatic acid phosphatase (TM‐PAP), a membrane‐bound splice variant of prostatic acid phosphatase, has ecto‐5′‐nucleotidase activity. Furthermore, it was demonstrated that ectophosphatase cannot hydrolyse ATP, ADP, or AMP in triple‐negative breast cancer cells. In contrast to previous findings in MDA‐MB‐231 cells, the ectophosphatase studied in the present work displayed a remarkable capacity to hydrolyse AMP in luminal A breast cancer cells (MCF‐7). We showed that AMP dose‐dependently inhibited p‐nitrophenylphosphate (p‐NPP) hydrolysis. The p‐NPP and AMP hydrolysis showed similar biochemical behaviours, such as increased hydrolysis under acidic conditions and comparable inhibition by NiCl2, ammonium molybdate, and sodium orthovanadate. In addition, this ectophosphatase with ectonucleotidase activity was essential for the release of adenosine and inorganic phosphate from phosphorylated molecules available in the extracellular microenvironment. This is the first study to show that prostatic acid phosphatase on the membrane surface of breast cancer cells (MCF‐7) is correlated with cell adhesion and migration.
Establishment and characterization of fibroblast lines from the northern tiger cat (Leopardus tigrinus, Schreber, 1775) during extended passage and cryopreservation
Authors: Viana João Vitor da Silva , de Oliveira Lhara Ricarliany Medeiros , Rodrigues Luanna Lorenna Vieira , Silva Yara Letícia Frutuoso e , Rodrigues Ana Lívia Rocha , Silva Alexandre Rodrigues , Alves Patrícia Vasconcelos , Silva Herlon Victor Rodrigues , Pereira Alexsandra Fernandes 
Published: September 2024

AbstractThe establishment of fibroblast lines enables several applications from the formation of biobanks for the conservation of biodiversity to the use of these cells in physiological and toxicological assays. Considered a species vulnerable to extinction, the characterization of fibroblastic lines of northern tiger cat would contribute to its conservation. Therefore, we established and characterized fibroblasts derived from northern tiger cat during extended passage (third, seventh, and eleventh passages) and cryopreservation with regard to the morphology, viability, apoptotic classification, metabolism, proliferative activity, and oxidative stress by reactive oxygen species (ROS) levels and mitochondrial membrane potential (ΔΨm). Initially, we identified four dermal fibroblast lines by morphology, immunophenotyping, and karyotyping assays. In vitro culture after the third, seventh, and eleventh passages did not affect the viability, apoptotic classification, and ROS levels. Nevertheless, cells at seventh and eleventh passages featured a reduction in metabolism and an alteration in ΔΨm when compared to third passage cells. Additionally, cells at eleventh passage showed changes in the proliferative activity and morphology when compared to other passages. Regarding cryopreservation, no effect was observed on cryopreserved cells for morphology, viability, apoptotic classification, metabolism, and proliferative activity. Nevertheless, cryopreserved cells had alteration for ROS levels and ΔΨm. In summary, fibroblasts from northern tiger cat were affected by extended passage (seventh and eleventh passages) and cryopreservation. Adjustments to the in vitro culture and cryopreservation are necessary to reduce cellular oxidative stress caused by in vitro conditions.
The m6A modification of ACSL4 mRNA sensitized esophageal squamous cell carcinoma to irradiation via accelerating ferroptosis
Authors: Jin Yingying , Pan Shupei , Wang Mincong , Huang Shan , Ke Yue , Li Dan , Luo Hen , Kou Zhanfeng , Shi Dongwen , Kou Weihua , Fu Hongxiao , Pan Jiyuan 
Published: September 2024

AbstractRadioresistance is a major obstacle for the therapy of esophageal squamous cell carcinoma (ESCC) and lead to a poor prognosis. Ferroptosis is supposed to be responsible for radioresistance. However, the ferroptosis‐induced radioresistance in ESCC and its related regulatory mechanisms are not yet fully understood. In this study, human ESCC cell line and the corresponding radioresistance cells were irradiated with 6 megavolts (MV) X‐ray. It was showed that irradiation led to less ferroptosis in radioresistant ESCC cells as compared to the parental cells, as depicted by transmission electron microscopy, intracellular Fe2+ iron contents, lipid peroxidation, and expression of COX2. The increase of ASCL4 expression levels in radioresistant cells after radiotherapy was smaller than that in the parental cells. ACSL4 overexpression significantly enhanced ferroptosis. The fold increase in ACSL4 m6A modification in the radioresistant cells was significantly smaller than that in the parental cells as detected by methylated RNA immunoprecipitation with qRT‐PCR. METTL14 overexpression accelerated ferroptosis induced by irradiation via upregulating m6A modification of ACSL4 mRNA. In conclusions, ferroptosis ablation was responsible for the radioresistant of ESCC. The METTL14‐mediated m6A modification of ACSL4 mRNA sensitized ESCC to irradiation via accelerating ferroptosis. This study sheds new light on our understanding of radioresistant in ESCC, and provides potential strategies for ESCC radiotherapy.
The impact of COVID‐19 on hay fever treatment in Japan: A retrospective cohort study based on the Japanese claims database
Authors: Akasaki Yasutsugu , Inomata Takenori , Iwagami Masao , Sung Jaemyoung , Nagino Ken , Adachi Takeya , Morita Hideaki , Tamari Mayumi , Kainuma Keigo , Kan‐o Keiko , Ogata Hiroaki , Sakashita Masafumi , Futamura Masaki , Kurashima Yosuke , Nakajima Saeko , Masaki Katsunori , Ogawa Yasushi , Sato Sakura , Miyagawa Akihiro , Midorikawa‐Inomata Akie , Fujimoto Keiichi , Okumura Yuichi , Fujio Kenta , Huang Tianxiang , Hirosawa Kunihiko , Morooka Yuki , Murakami Akira , Nakao Shintaro 
Published: September 2024

AbstractBackgroundHay fever (HF) presents with various symptoms, including allergic conjunctivitis and rhinitis, and requires cross‐organ treatment. This study assessed the impact of the coronavirus disease 2019 (COVID‐19) pandemic on HF treatment trends.MethodsThis retrospective cohort study utilized data from the JMDC database collected between January 2018 and May 2021. Patients with HF were identified based on the relevant International Classification of Diseases 10th Revision diagnosis codes and the prescription of HF‐related medications. The treatment approaches were compared during the cedar and cypress pollen allergy season (January to May in Japan) before and during the COVID‐19 pandemic (2018 and 2019, and 2020 and 2021, respectively).ResultsThis study included 2,598,178 patients with HF. The numbers of prescribed HF‐related claims in 2018, 2019, 2020, and 2021 were 3,332,854, 3,534,198, 2,774,380, and 2,786,681 times, respectively. Oral second‐generation antihistamine prescriptions decreased by >10% from 2019 to 2020, with a <10% change in the subsequent year. Anti‐allergic eye drop prescriptions also decreased by >10% from 2019 to 2020 but increased by >10% from 2020 to 2021. Compared with 2018, 2019, and 2020, the number of claims in the rhinitis symptoms dominant group was significantly decreased in 2021 (p < 0.001, all). In contrast, the number of claims in the eye symptoms dominant group and the rhinitis and eye symptoms dominant group increased in 2021 compared with that in 2018, 2019, and 2020 (p < 0.001, all).ConclusionChanges in HF treatment and related outcomes could be attributed to lifestyle modifications resulting from the COVID‐19 pandemic. Measures, such as limiting outdoor activities and adopting mask‐wearing practices may have influenced HF symptoms, preventive behaviors, and the overall approach to treating HF.
Lightweight High‐Throughput TRNG Based on Single‐Node Boolean Chaotic Structure
Authors: Zhou Hongli , Yao Liang , Feng Yongkang , Huang Zhengfeng , Lu Yingchun 
Published: September 2024

ABSTRACTWith the rise of the Internet and electronic devices, the security of network information is gaining attention, and the true random number generator (TRNG) is playing an increasingly crucial role in information security. TRNG, based on Boolean chaotic entropy source, has drawn significant interest due to its uncomplicated circuit design and minimal hardware resource usage. However, most existing structures consist of two‐input or three‐input logic devices, forming a complex multinode, geometrically symmetric Boolean chaotic network using multiple logic devices. This network configuration results in increased complexity and reduced throughput. This study introduces an entropy source based on Boolean chaos utilizing single‐node and four‐input XOR gates, which can be easily placed and routed on Xilinx Artix‐7 FPGA. It requires only 29 LUTs and 5 DFFs without any postprocessing, achieving a throughput of up to 700 Mb/s. The output of TRNG has successfully passed various tests including the autocorrelation test, NIST SP800‐22, NIST SP800‐90B, AIS‐31, and TESTU01 tests with favorable results. Furthermore, by applying a three‐stage XOR chain postprocessing on Xilinx Spartan‐6 FPGA and Xilinx Virtex‐6 FPGA, it has passed the NIST SP800‐22 and NIST SP800‐90B tests at 300 Mb/s. The structure was also tested using Xilinx Virtex‐6 FPGA under different temperature and voltage conditions, passing the NIST SP800‐90B IID test.
Effect of spray COAG mode on hemostasis in colorectal endoscopic submucosal dissection using inverse probability of treatment weight analysis
Authors: Kanazawa Jun , Ikehara Hisatomo , Horii Toshiki , Kitahara Gen , Betto Tomohiro , Yokoyama Kaoru , Kobayashi Kiyonori , Kusano Chika 
Published: September 2024

AbstractObjectiveSwift and forced COAG with an electrosurgical knife are commonly used for intraoperative hemostasis in colorectal endoscopic submucosal dissection (ESD). If bleeding cannot be stopped using an electrosurgical knife, cauterization is attempted using hemostatic forceps. Since April 2022, our hospital has started using Spray COAG for intraoperative hemostasis for colorectal ESD. This study aimed to provide evidence of the efficacy of Spray COAG for intraoperative hemostasis.MethodsColorectal ESD was performed for 320 lesions at our hospital. Of these, 307 were included; 145 and 162 lesions were operated before and after the introduction of Spray COAG, respectively. Spray COAG was used after the change. The primary endpoint was the change in the frequency of use of hemostatic forceps after the introduction of Spray COAG; the secondary endpoint was the change in the prevalence of postoperative complications after the introduction of Spray COAG. It should be noted that the Spray COAG mode was employed solely for hemostasis and not for dissection, while the Swift COAG mode was utilized for dissection in the After Spray COAG group. Statistical analysis was conducted using IPTW analysis.ResultsThe frequency of use of hemostatic forceps was significantly decreased after the introduction of Spray COAG (odds ratio = 0.12, 95% confidence interval [95%CI]: 0.06–0.23, p < 0.001). The prevalence of post‐ESD electrocoagulation syndrome significantly decreased (odds ratio = 0.43, 95%CI: 0.22–0.88, p = 0.02). No significant differences were observed between the intraoperative and postoperative perforations or rate of postoperative bleeding.ConclusionSpray COAG reduced the frequency of hemostatic forceps use in colorectal ESD.