bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antidepressants and Driving in Older Adults: A Systematic Review
Authors: Cameron Duncan H. , Rapoport Mark J. 
Published: April 2016

RÉSUMÉAvec le nombre croissant de conducteurs âgés pour lesquelles on prescrit des antidépresseurs, les conséquences potentielles de l’utilisation des antidépresseurs sur les compétences de conduite dans une population vieillissante deviennent un problème urgent. Nous avons effectué une analyse systématique en utilisant MEDLINE, ciblant des articles qui se rapportent spécifiquement aux antidépresseurs et à la conduite dans une population ou sous-groupe de personnes âgées (≥ 55 ans d’âge). La recherche a retourné 267 références, dont neuf portaient sur les effets des antidépresseurs sur la conduite chez les personnes âgées. L’étude expérimentale unique a trouvé que imipramine exerce des effets néfastes sur la conduite sur autoroute, alors que la néfazodone n’a pas fait. Sept des huit études de population ont rapporté une augmentation significative du risque d’être impliqué dans une collision associé à l’utilisation des antidépresseurs. Bien que les études ont indiqué un effet négatif des antidépresseurs sur la conduite, les conceptions épidémiologiques ne peuvent pas exclure la possibilité que la maladie sous-jacente, qui est généralement la dépression majeure, est la coupable.
Genetic encoding of DNA nanostructures and their self-assembly in living bacteria
Authors: Elbaz Johann , Yin Peng , Voigt Christopher A. 
Published: April 2016

AbstractThe field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32–205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 3′-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 μm2) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells.
Dynamic and flexible H3K9me3 bridging via HP1β dimerization establishes a plastic state of condensed chromatin
Authors: Hiragami-Hamada Kyoko , Soeroes Szabolcs , Nikolov Miroslav , Wilkins Bryan , Kreuz Sarah , Chen Carol , De La Rosa-Velázquez Inti A. , Zenn Hans Michael , Kost Nils , Pohl Wiebke , Chernev Aleksandar , Schwarzer Dirk , Jenuwein Thomas , Lorincz Matthew , Zimmermann Bastian , Walla Peter Jomo , Neumann Heinz , Baubec Tuncay , Urlaub Henning , Fischle Wolfgang 
Published: April 2016

AbstractHistone H3 trimethylation of lysine 9 (H3K9me3) and proteins of the heterochromatin protein 1 (HP1) family are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1 contribute to chromatin condensation have been speculative and controversial. Here we demonstrate that human HP1β is a prototypic HP1 protein exemplifying most basal chromatin binding and effects. These are caused by dimeric and dynamic interaction with highly enriched H3K9me3 and are modulated by various electrostatic interfaces. HP1β bridges condensed chromatin, which we postulate stabilizes the compacted state. In agreement, HP1β genome-wide localization follows H3K9me3-enrichment and artificial bridging of chromatin fibres is sufficient for maintaining cellular heterochromatic conformation. Overall, our findings define a fundamental mechanism for chromatin higher order structural changes caused by HP1 proteins, which might contribute to the plastic nature of condensed chromatin.
Dispersion and shape engineered plasmonic nanosensors
Authors: Jeong Hyeon-Ho , Mark Andrew G. , Alarcón-Correa Mariana , Kim Insook , Oswald Peter , Lee Tung-Chun , Fischer Peer 
Published: April 2016

AbstractBiosensors based on the localized surface plasmon resonance (LSPR) of individual metallic nanoparticles promise to deliver modular, low-cost sensing with high-detection thresholds. However, they continue to suffer from relatively low sensitivity and figures of merit (FOMs). Herein we introduce the idea of sensitivity enhancement of LSPR sensors through engineering of the material dispersion function. Employing dispersion and shape engineering of chiral nanoparticles leads to remarkable refractive index sensitivities (1,091 nm RIU−1 at λ=921 nm) and FOMs (>2,800 RIU−1). A key feature is that the polarization-dependent extinction of the nanoparticles is now characterized by rich spectral features, including bipolar peaks and nulls, suitable for tracking refractive index changes. This sensing modality offers strong optical contrast even in the presence of highly absorbing media, an important consideration for use in complex biological media with limited transmission. The technique is sensitive to surface-specific binding events which we demonstrate through biotin–avidin surface coupling.
Calorimetry of a Bose–Einstein-condensed photon gas
Authors: Damm Tobias , Schmitt Julian , Liang Qi , Dung David , Vewinger Frank , Weitz Martin , Klaers Jan 
Published: April 2016

AbstractPhase transitions, as the condensation of a gas to a liquid, are often revealed by a discontinuous behaviour of thermodynamic quantities. For liquid helium, for example, a divergence of the specific heat signals the transition from the normal fluid to the superfluid state. Apart from liquid helium, determining the specific heat of a Bose gas has proven to be a challenging task, for example, for ultracold atomic Bose gases. Here we examine the thermodynamic behaviour of a trapped two-dimensional photon gas, a system that allows us to spectroscopically determine the specific heat and the entropy of a nearly ideal Bose gas from the classical high temperature to the Bose-condensed quantum regime. The critical behaviour at the phase transition is clearly revealed by a cusp singularity of the specific heat. Regarded as a test of quantum statistical mechanics, our results demonstrate a quantitative agreement with its predictions at the microscopic level.
Dynamic and quantitative assessment of blood coagulation using optical coherence elastography
Authors: Xu Xiangqun , Zhu Jiang , Chen Zhongping 
Published: April 2016

AbstractReliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator and blood spiked with fibrinogen.
Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4
Authors: Yang Jinju , Qin Nannan , Zhang Hongwei , Yang Rui , Xiang Benqiong , Wei Qun 
Published: April 2016

AbstractOur previous research showed that recombinant calcineurin B (rhCnB) stimulates cytokine secretion by immune cells, probably through TLR4. Exogenous CnB can be incorporated into many different tumour cellsin vitro, but the mode of uptake and receptors required remain unknown. Here, we report that exogenous CnB is taken up by cells in a time- and concentration-dependent manner via clathrin-dependent receptor-mediated internalization. Our findings further confirm that uptake is mediated by the TLR4/MD2 complex together with the co-receptor CD14. The MST results revealed a high affinity between CnB and the TLR4 receptor complex. No binding was detected between CnB and LPS. CnB inhibited the uptake of LPS, and LPS also inhibited the uptake of CnB. These results indicate that the uptake of exogenous CnB did not occur through LPS and that CnB was not a chaperone of LPS. Thus, we conclude that TLR4 receptor complexes were required for the recognition and internalization of exogenous CnB. CnB could be a potential endogenous ligand of TLR4 and function as an agonist of TLR4. These properties of CnB support its potential for development as an anti-cancer drug.
A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells
Authors: Kim Kyoung-Jin , Kim Hee-Jin , Park Han-Gyu , Hwang Cheol-Hwan , Sung Changmin , Jang Kyoung-Soon , Park Sung-Hee , Kim Byung-Gee , Lee Yoo-Kyung , Yang Yung-Hun , Jeong Jae Hyun , Kim Yun-Gon 
Published: April 2016

AbstractThe level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based quantitative platform to identify the absolute quantities of endogenous estrones in a variety of clinical specimens. The one-step modification of endogenous estrone provided good linearity (R2 > 0.99) and significantly increased the sensitivity of the platform (limit of quantitation: 11 fmol). In addition, we could identify the absolute amount of endogenous estrones in cells of the breast cancer cell line MCF-7 (34 fmol/106 cells) by using a deuterated estrone as an internal standard. Finally, by applying the MALDI-MS-based quantitative method to endogenous estrones, we successfully monitored changes in the metabolic expression level of estrones (17.7 fmol/106 letrozole-treated cells) in MCF-7 cells resulting from treatment with an aromatase inhibitor. Taken together, these results suggest that this MALDI-MS-based quantitative approach may be a general method for the targeted metabolomics of ketone-containing metabolites, which can reflect clinical conditions and pathogenic mechanisms.
Three-dimensional multiway power dividers based on transformation optics
Authors: Wu Yong-Le , Zhuang Zheng , Deng Li , Liu Yuan-An 
Published: April 2016

AbstractThe two-dimensional (2D) or three-dimensional (3D) multiway power dividers based on transformation optical theory are proposed in this paper. It comprises of several nonisotropic mediums and one isotropic medium without any lumped and distributed elements. By using finite embedded coordinate transformations, the incident beam can be split and bent arbitrarily in order to achieve effective power division and transmission. In addition, the location of the split point can be employed to obtain unequal power dividers. Finally, several typical examples of the generalized power divider without limitation in 3D space are performed, which shows that the proposed power divider can implement required functions with arbitrary power division and arbitrary transmission paths. The excellent simulated results verify the novel design method for power dividers.
N7-(carboxymethyl)guanine-Lithium Crystalline Complex: A Bioinspired Solid Electrolyte
Authors: Dutta Dipak , Nagapradeep N. , Zhu Haijin , Forsyth Maria , Verma Sandeep , Bhattacharyya Aninda J. 
Published: April 2016

AbstractElectrochemical device with components having direct significance to biological life processes is a potent futuristic strategy for the realization of all-round green and sustainable development. We present here synthesis design, structural analysis and ion transport of a novel solid organic electrolyte (G7Li), a compound reminiscent of ion channels, derived from regioisomeric N7-guanine-carboxylate conjugate and Li-ions. G7Li, with it’s in-built supply of Li+-ions, exhibited remarkably high lithium-ion transference number (= 0.75) and tunable room temperature ionic conductivity spanning three decades (≈10−7to 10−3 Ω−1 cm−1) as a function of moisture content. The ionic conductivity show a distinct reversible transition around 80–100 °C, from a dual Li+and H+(<100 °C) to a pure Li+conductor (>100 °C). Systematic studies reveal a transition from water-assisted Li-ion transport to Li hopping-like mechanism involving guanine-Li coordination. While as-synthesized G7Li has potential in humidity sensors, the anhydrous G7Li is attractive for rechargeable batteries.
Isoform switching and exon skipping induced by the DNA methylation inhibitor 5-Aza-2′-deoxycytidine
Authors: Ding Xiao-Lei , Yang Xiaojing , Liang Gangning , Wang Kai 
Published: April 2016

AbstractDNA methylation in gene promoters leads to gene silencing and is the therapeutic target of methylation inhibitors such as 5-Aza-2′-deoxycytidine (5-Aza-CdR). By analyzing the time series RNA-seq data (days 5, 9, 13, 17) obtained from human bladder cells exposed to 5-Aza-CdR with 0.1 uM concentration, we showed that 5-Aza-CdR can affect isoform switching and differential exon usage (i.e., exon-skipping), in addition to its effects on gene expression. We identified more than 2,000 genes with significant expression changes after 5-Aza-CdR treatment. Interestingly, 29 exon-skipping events induced by treatment were identified and validated experimentally. Particularly, exon-skipping event in Enhancer of Zeste Homologue 2 (EZH2) along with expression changes showed significant down regulation on Day 5 and Day 9 but returned to normal level on Day 13 and Day 17. EZH2 is a component of the multi-subunit polycomb repressive complex PRC2, and the down-regulation of exon-skipping event may lead to the regain of functional EZH2 which was consistent with our previous finding that demethylation may cause regain of PRC2 in demethylated regions. In summary, our study identified pervasive transcriptome changes of bladder cancer cells after treatment with 5-Aza-CdR, and provided valuable insights into the therapeutic effects of 5-Aza-CdR in current clinical trials.
Health Effects of PCBs in Residences and Schools (HESPERUS): PCB – health Cohort Profile
Authors: Bräuner Elvira Vaclavik , Andersen Zorana Jovanovic , Frederiksen Marie , Specht Ina Olmer , Hougaard Karin Sørig , Ebbehøj Niels , Bailey Janice , Giwercman Aleksander , Steenland Kyle , Longnecker Matthew Paul , Bonde Jens Peter 
Published: April 2016

AbstractPolychlorinated-biphenyls (PCBs) were introduced in the late 1920s and used until the 1970s when they were banned in most countries due to evidence of environmental build-up and possible adverse health effects. However they still persist in the environment, indoors and in humans. Indoor air in contaminated buildings may confer airborne exposure markedly above background regional PCB levels. To date, no epidemiological studies have assessed the health effects from exposure to semi-volatile PCBs in the indoor environment. Indoor air PCBs are generally less chlorinated than PCBs that are absorbed via the diet, or via past occupational exposure; therefore their health effects require separate risk assessment. Two separate cohorts of individuals who have either attended schools (n = 66,769; 26% exposed) or lived in apartment buildings (n = 37,185; 19% exposed), where indoor air PCB concentrations have been measured were created. An individual estimate of long-term airborne PCB exposure was assigned based on measurements. The cohorts will be linked to eight different national data sources on mortality, school records, residential history, socioeconomic status, and chronic disease and reproductive outcomes. The linking of indoor air exposures with health outcomes provides a dataset unprecedented worldwide. We describe a project, called HESPERUS (Health Effects of PCBs in Residences and Schools), which will be the first study of the long term health effects of the lower-chlorinated, semi-volatile PCBs in the indoor environment.
Refinement and growth enhancement of Al2Cu phase during magnetic field assisting directional solidification of hypereutectic Al-Cu alloy
Authors: Wang Jiang , Yue Sheng , Fautrelle Yves , Lee Peter D. , Li Xi , Zhong Yunbo , Ren Zhongming 
Published: April 2016

AbstractUnderstanding how the magnetic fields affect the formation of reinforced phase during solidification is crucial to tailor the structure and therefor the performance of metal matrix in situ composites. In this study, a hypereutectic Al-40 wt.%Cu alloy has been directionally solidified under various axial magnetic fields and the morphology of Al2Cu phase was quantified in 3D by means of high resolution synchrotron X-ray tomography. With rising magnetic fields, both increase of Al2Cu phase’s total volume and decrease of each column’s transverse section area were found. These results respectively indicate the growth enhancement and refinement of the primary Al2Cu phase in the magnetic field assisting directional solidification. The thermoelectric magnetic forces (TEMF) causing torque and dislocation multiplication in the faceted primary phases were thought dedicate to respectively the refinement and growth enhancement. To verify this, a real structure based 3D simulation of TEMF in Al2Cu column was carried out and the dislocations in the Al2Cu phase obtained without and with a 10T high magnetic field were analysed by the transmission electron microscope.
Universal lineshapes at the crossover between weak and strong critical coupling in Fano-resonant coupled oscillators
Authors: Zanotto Simone , Tredicucci Alessandro 
Published: April 2016

AbstractIn this article we discuss a model describing key features concerning the lineshapes and the coherent absorption conditions in Fano-resonant dissipative coupled oscillators. The model treats on the same footing the weak and strong coupling regimes, and includes the critical coupling concept, which is of great relevance in numerous applications; in addition, the role of asymmetry is thoroughly analyzed. Due to the wide generality of the model, which can be adapted to various frameworks like nanophotonics, plasmonics, and optomechanics, we envisage that the analytical formulas presented here will be crucial to effectively design devices and to interpret experimental results.
Hospitalization risk factors for children’s lower respiratory tract infection: A population-based, cross-sectional study in Mongolia
Authors: Dagvadorj Amarjargal , Ota Erika , Shahrook Sadequa , Baljinnyam Olkhanud Purevdorj , Takehara Kenji , Hikita Naoko , Bavuusuren Bayasgalantai , Mori Rintaro , Nakayama Takeo 
Published: April 2016

AbstractThis study aimed to assess the potential risk factors for lower respiratory tract infection (LRTI)-related hospital admissions in Mongolian children. A population-based cross-sectional study was conducted in rural Mongolia in 2013, and 1,013 mother–child pairs were included. Of the participating children, 38.9% were admitted to hospital with LRTIs. Home smoking, low birthweight, being a male child, exclusive breastfeeding and healthcare-seeking behaviour showed substantial association with LRTI-related hospital admissions. Number of cigarettes smoked by family members showed a dose-response relationship and increased hospital admissions. Strategies to prevent second-hand-smoke exposure from adult smokers, especially inside the home, are crucial to preventing LRTI-related hospital admissions for children in Mongolia. Improving rates of exclusive breastfeeding and increasing birthweight have great potential to decrease the likelihood of children acquiring a LRTI. Educational initiatives are also necessary for women who are less likely to seek out care for their children’s symptoms.
Ordered Monolayer Gold Nano-urchin Structures and Their Size Induced Control for High Gas Sensing Performance
Authors: Sabri Ylias M. , Kandjani Ahmad Esmaielzadeh , Ippolito Samuel J. , Bhargava Suresh K. 
Published: April 2016

AbstractThe synthesis of ordered monolayers of gold nano-urchin (Au-NU) nanostructures with controlled size, directly on thin films using a simple electrochemical method is reported in this study. In order to demonstrate one of the vast potential applications, the developed Au-NUs were formed on the electrodes of transducers (QCM) to selectively detect low concentrations of elemental mercury (Hg0) vapor. It was found that the sensitivity and selectivity of the sensor device is enhanced by increasing the size of the nanospikes on the Au-NUs. The Au-NU-12 min QCM (Au-NUs with nanospikes grown on it for a period of 12 min) had the best performance in terms of transducer based Hg0 vapor detection. The sensor had 98% accuracy, 92% recovery, 96% precision (repeatability) and significantly, showed the highest sensitivity reported to date, resulting in a limit of detection (LoD) of only 32 μg/m3 at 75 °C. When compared to the control counterpart, the accuracy and sensitivity of the Au-NU-12 min was enhanced by ~2 and ~5 times, respectively. The results demonstrate the excellent activity of the developed materials which can be applied to a range of applications due to their long range order, tunable size and ability to form directly on thin-films.
The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns
Authors: Hadj-Moussa Hanane , Moggridge Jason A. , Luu Bryan E. , Quintero-Galvis Julian F. , Gaitán-Espitia Juan Diego , Nespolo Roberto F. , Storey Kenneth B. 
Published: April 2016

AbstractWhen faced with adverse environmental conditions, the marsupial Dromiciops gliroides uses either daily or seasonal torpor to support survival and is the only known hibernating mammal in South America. As the sole living representative of the ancient Order Microbiotheria, this species can provide crucial information about the evolutionary origins and biochemical mechanisms of hibernation. Hibernation is a complex energy-saving strategy that involves changes in gene expression that are elicited in part by microRNAs. To better elucidate the role of microRNAs in orchestrating hypometabolism, a modified stem-loop technique and quantitative PCR were used to characterize the relative expression levels of 85 microRNAs in liver and skeletal muscle of control and torpid D. gliroides. Thirty-nine microRNAs were differentially regulated during torpor; of these, 35 were downregulated in liver and 11 were differentially expressed in skeletal muscle. Bioinformatic analysis predicted that the downregulated liver microRNAs were associated with activation of MAPK, PI3K-Akt and mTOR pathways, suggesting their importance in facilitating marsupial torpor. In skeletal muscle, hibernation-responsive microRNAs were predicted to regulate focal adhesion, ErbB, and mTOR pathways, indicating a promotion of muscle maintenance mechanisms. These tissue-specific responses suggest that microRNAs regulate key molecular pathways that facilitate hibernation, thermoregulation, and prevention of muscle disuse atrophy.