bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oily Fish Intake and Cardiovascular Diseases: A Mendelian Randomization Study
Authors: Liu Xin , Chen Ankun , Lou Yating , Zhang Chengcheng , YUE Zenghui 
Published: September 2024

Abstract

Studies have shown a link between oily fish intake and a lower risk of cardiovascular disease (CVD). The potential causal relationship is unclear. The purpose of this study was to investigate the association between oily fish intake and eight CVDs, including coronary heart disease (CHD), heart failure (HF), myocardial infarction (MI), atrial fibrillation (AF), essential primary hypertension (EH), stroke, deep venous thrombosis (DVT), and peripheral artery disease (PDA), through a two-step Mendelian randomization (MR). Genome-wide association study (GWAS) statistics for oily fish intake and CVD were collected from the UK Biobanks and the European Bioinformatics Institute. Single nucleotide polymorphisms (SNP) are used as instrumental variables. In this analysis, the methods for evaluating causality were the inverse-variance weighted, weighted median, and simple median. To evaluate the consistency and dependability of the findings, sensitivity analyses and heterogeneity tests are carried out. MR analysis indicated that genetically predicted oily fish intake is associated with reduced risk of CHD (OR = 0.43, 95% CI, 0.27–0.71, p = 0.0009) and HF (OR = 0.79, 95% CI, 0.65–0.97, p = 0.0245).However, there was no association observed between oily fish intake and MI (OR = 1.00, 95% CI, 0.99–1.01, p = 0.9089), AF (OR = 0.95, 95% CI, 0.79–1.14, p = 0.5475), stroke (OR = 0.99, 95% CI, 0.99–1.00, p = 0.0116), EH (OR = 1.00, 95% CI, 1.00–1.00, p = 0.0009), DVT (OR = 1.00, 95% CI, 0.99–1.01, p = 0.5407), and PAD (OR = 1.00, 95% CI, 0.99–1.00, p = 0.2819). This MR study found a causal connection between oily fish intake and a lower incidence of CHD and HF, but did not affect MI, AF, stroke, EH, DVT, or PAD.
Differential phytohormone related senescence regulation in main and lateral roots of longan (Dimocarpus longan Lour.)
Authors: Liu Lin , Liu Liqin , Shi Shengyou , Shu Bo 
Published: September 2024

Abstract

Root senescence (RS) is a common phenomenon in longan, however, the regulation of RS is poorly understood. In this study, the main root was divided into three segments from tip to crown, i.e., distal (MDR), middle (MMR) and proximal (MPR) parts, and lateral roots emerging from the corresponding segments of the main root, i.e., lateral roots from the distal (LDR), middle (LMR) and proximal (LPR) parts, were used for analysis of morphological characteristics, physiological root activity, transcriptome, proteome, and phytohormone quantification, in order to understand the regulation of RS in longan seedlings. The root hair and epidermis of the proximal parts of the main and lateral roots exhibited senescence, and the activity decreased from distal to the proximal parts, suggesting that both main and lateral roots underwent senescence. The transcriptome profile showed that 2692 and 1406 differentially expressed genes were involved in RS of main and lateral roots, respectively, and proteome data exhibited 222 and 124 differentially accumulated proteins involved in RS of main and lateral roots, respectively, based on MPR vs MMR and LPR vs LMR groups. Based on the correlation analysis of DAPs and DEGs, the KEGG pathways of carotenoid biosynthesis (abscisic acid) and steroid biosynthesis (brassinosteroid) were found to be significantly different in the main roots, and two KEGG pathways related to plant hormones, i.e., phenylalanine metabolism (salicylic acid), cysteine and methionine metabolism (ethylene) were significant in lateral roots. Further, the abscisic acid concentration of MMR was 9.47% lower than that of MPR, and it was negative related with root activity of main root. The concentration of 1-aminocyclopropane-1-carboxylic acid was increased 1.59 times from LDR to LPR, and it was negative related with activity of lateral root. Thus, this study indicates that different phytohormones are related to senescence regulation in the main and lateral roots of longan seedlings. In addition, transcriptome and proteome data furnish basic information related to RS of longan seedlings.
Early Childhood Adversity Predicts Risk of Family Caregiver Suicidal Ideation:Roles of Neuroticism and Self-Compassion
Authors: Jain Felipe A. , Gutierrez-Ramirez Paulina , Zea Miranda , Okereke Olivia I. , Orden Kimberly A. Van , Pedrelli Paola , Vranceanu Ana-Maria , Dueck Kimberly , Pederson Aderonke , Gomez Liliana A. Ramirez 
Published: September 2024

Abstract

Background: Despite high rates of family caregiver suicidal ideation (SI), little is known about its relationship with childhood adversity. Those with a history of adverse childhood experiences (ACEs) have been shown to have higher neuroticism, lower self-compassion, and higher rates of late life mental health disorders. Caregiving for a family member with dementia may pose a particular challenge for those with ACEs.
Methods: In a secondary analysis of 81 family caregivers of people living with dementia enrolled in clinical trials, we undertook a cross-sectional baseline analysis of the association between childhood adversity, measured with the ACE questionnaire, and self-reported suicidal ideation (SI). We further assessed whether the relationship between ACE and SI was mediated by neuroticism and self-compassion.
Results: 18 caregivers self-reported SI (22%). 89% of caregivers with SI reported childhood adversity (ACE > 0), versus 63% of those without SI (p=.04). The relative risk of SI was 3.6x higher in those with childhood adversity than in those without (p=.04), and for those with a specific history childhood abuse, the relative risk of SI was 3.4x higher (p=.005). Neuroticism and self-compassion mediated the relationship between ACE and SI (p<.05), with neuroticism strengthening the association and self-compassion weakening it.
Conclusions: The association of SI with history of childhood adversity is high in family caregivers. Whereas elevated neuroticism might be one mechanism linking ACEs and SI, training self-compassion is a promising target for reducing SI. The phenotypic relationship between childhood adversity and SI in family caregivers should be further explored in larger samples, and could represent a new treatment target to improve the efficacy of therapies on caregiver emotional symptoms.
Nutritional characterization of patients with oropharyngeal cancers: Impact of human papillomavirus status
Authors: Baracos Vicky , Choulli Maryam , Kubrak Catherine , Morey Francisca , Brenes Jesús , Tous Sara , Quirós Beatriz , Wang Xin , Pavón Miquel , Gomà Montserrat , Taberna Miren , Alemany Laia , Oliva Marc , Mena Marisa , Jha Naresh , Scrimger Rufus , Debenham Brock , Chua Neil , Walker John , Mesia Ricard , Arribas Lorena 
Published: September 2024

Abstract

Background
Oropharyngeal squamous cell carcinoma (OPSCC) of human papillomavirus (HPV)-positive status is increasing relative to HPV-negative disease. Nutritional features of OPSCC patients according to HPV status is unclear.
Subjects/Methods:
Canadian and Spanish patients with OPSCC were assessed for body mass index (BMI), weight loss grade (WLG), and computed tomography-defined skeletal muscle index (SMI). Chi-square, t-test, Mann-Whitney-U, Kruskal-Wallis tests were conducted to compare HPV positive and negative groups. Overall survival (OS) was assessed by univariable Kaplan-Meier and Cox proportional hazard methods.
Results
No differences in BMI, WLG, SMI and adipose tissue index between the 308 (Canada) and 134 (Spain) patients according to HPV status; hence cohorts were pooled (n = 442). HPV-positive patients (n = 317) were overweight/obese (72.8%), had WLG of 0/1 (59.6%) and high SMI (83.4%) while HPV-negative patients were normal/underweight (61.5%), had high WLG 3/4 (50.8%), and moderate/severe SMI depletion (46.9%)(p < 0.003). These overall differences notwithstanding, there was crossover i.e. 35% of HPV-positive patients had high WLG and/or moderate/severe muscle depletion and 29% of HPV-negative patients had minimal weight loss and high SMI. HPV-negative patients had a higher risk of mortality (HR 3.78, 95% CI 2.70 to 5.29, P < 0.001) and this difference was retained after multivariable adjustment for WLG, SMI, age, and disease stage (HR 3.19, 95% CI 2.12 to 4.79, P < 0.001).
Conclusion
Nutrition features of patients with OPSCC did not differ between Canada and Spain. Distinctive nutrition features exist in patients according to HPV status. The high heterogeneity of individual nutritional profiles invites an individualized approach to nutrition care.
In Vitro and In Vivo Evaluation of Diosmetin-loaded Lactoferrin-Modified Liposomes with Enhanced Brain-Targeting Effects for Intracerebral Hemorrhage
Authors: Gu Yingjiang , Luo Hanyue , Zhu Jun , Ma Hao , Zhang Yang , Xing Jinshan , Liu Yuzhou , Cai Yu , Sun Wenxia , LUO Pei 
Published: September 2024

Abstract

Intracerebral hemorrhage (ICH) is a serious cerebrovascular disease with high morbidity, mortality, and disability rates, largely due to neuroinflammation. Diosmetin, a natural flavonoid, has known neuroprotective effects in cerebral ischemia/reperfusion models but has been less studied in ICH. Our previous study developed diosmetin-loaded lactoferrin-modified long-circulating liposomes (Lf-Dios-Lcl), which penetrate the BBB and improve diosmetin bioavailability and brain distribution. In this study, we found that diosmetin reduced the levels of proinflammatory cytokines (IL-1β and TNF-α) and increased the level of the anti-inflammatory cytokine IL-10 in LPS-induced BV2 cells, promoting microglial polarization toward the anti-inflammatory M2 phenotype. In ICH model rats, Lf-Dios-Lcl (1 mg/kg) effectively reduced neuroinflammation, decreased IL-1β and TNF-α levels, increased IL-10 levels, and increased the proportion of CD206-positive microglia in brain tissues. Moreover, Lf-Dios-Lcl significantly downregulated p-p38 expression, suggesting that p38 signaling activation was inhibited. Overall, Lf-Dios-Lcl demonstrated brain-targeting properties and antineuroinflammatory effects by modulating microglial polarization via the p38 pathway.
KAISO Promotes Poor Prognosis in Hepatocellular Carcinoma Patients by Enhancing Neutrophil Infiltration via IGFBP1
Authors: Zhou Jiang , Pang Yiqiang , Wang Haojun , Wang Yatian , Li Quan , Yang Tongwang 
Published: September 2024

Abstract

Background
KAISO is a transcriptional regulator involved in gene expression, cell proliferation, and apoptosis, linked to cancer prognosis and tumor aggressiveness, making it a potential bi-omarker and therapeutic target.
Methods: We used bioinformatics analyses to evaluate KAISO expression and its effect on survival prognosis across 33 types of pan-cancer. We also examined the link between KAISO expression and immune cell infiltration. To investigate the control of down-stream proteins by KAISO, we used dual-luciferase reporter assays, electrophoretic mobility shift assays (EMSA), and chromatin immunoprecipitation (ChIP). Additionally, we validated the role of KAISO in regulating immune cell infiltration using a subcutaneous tumor model in animals and human tumor samples.
Results: Our research revealed that KAISO is crucial in regulating the growth and progression of various malignancies, including hepatocellular carcinoma (HCC). We demonstrated that high KAISO expression is associated with poor prognosis in HCC. KAISO was found to regulate the transcription of IGFBP1 and neutrophil infiltration and influence HCC pro-liferation through cell cycle-related molecular pathways. Finally, we confirmed that reducing KAISO expression can inhibit neutrophil infiltration and tumor growth.
Conclusion: Our findings suggest that KAISO could be an important biomarker and molecular target for HCC patients.
New conjunctival forceps combined with high-frequency electrocautery-assisted correction of conjunctivochalasis
Authors: Song Tingting , Liang Xianjun , Lin Yingjie , Luo Huihui 
Published: September 2024

Abstract

Purpose: To investigate the efficiency, efficacy, and safety of new conjunctival forceps combined with high-frequency electrocautery-assisted correction of conjunctivochalasis (CCh).
Methods: 19 patients with CCh, totaling 22 eyes, were included in the study. The forceps have a serrated tapered head and a latch for firm tissue grip. The conjunctiva was secured for resection using the forceps latch, grasped 3-5 mm from the limbus, and treated clockwise. Direct electrocautery was utilized for lifts ≤3 mm; for heights >3 mm, forceps were used to clamp, scissors were used for excision, and the electrode tip was used to close the wound following anesthesia. Surgical duration was recorded, and CCh severity, assessed with lip-parallel conjunctival folds (LIPCOF), along with discomfort scored by Ocular Surface Disease Index (OSDI), were compared pre- and post-operatively. Healing and complications were checked at one week and one month.
Results: Surgery averaged 6.95 minutes. Initially, 18.2% of the patients were LIPCOF Grade I, 22.3% were Grade II, and 59.1% were Grade III. Postoperatively, 59.1% reached grade 0 at one week, and 95.5% by one month. The OSDI scores significantly improved from week one to month one (P<0.05), falling from 40.06 to 17.43 in a single month. Healing was complete within one week for cauterized areas, one month for excision. Minor congestion and edema at one week were cleared by one month with no major issues.
Conclusion: The new conjunctival forceps with high-frequency electrocautery for CCh correction proved to be efficient, effective, and safe.
Assessing impacts of rainfall intensity and slope gradient on runoff process and dissolved organic carbon loss via surface flow and interflow under simulated rainfall
Authors: Xu Lu , Lu Jun , Zhang Dan 
Published: September 2024

Abstract

Dissolved organic carbon (DOC) is an indispensable component of the global carbon cycle and potentially affects aquatic ecosystems. Previous research on runoff process and DOC loss mainly focused on surface flow, with few reports of the hydrological pathway of interflow or DOC loss via interflow. To address this deficiency, a series of rainfall simulations were conducted with three rainfall intensities of 60, 90, and 120 mm h− 1 (R60, R90, and R120) and three slope gradients of 5, 15, and 25° (S5, S15, and S25) of purplish soil. The initial time of surface flow was faster under high rainfall intensity and steep slope, and the initial time of interflow increased with increased rainfall intensity under gentle slope. In general, the surface flow rates increased first, and reached a steady state within 10–35 min. The interflow curves were single-peak curves for R60-S5 and R90-S5, but exhibited a continued rising trend for other treatments. The interflow volume occupied 69.2% of the total runoff volume under R60-S5, and the percentages of interflow decreased as the rainfall intensity and slope increased. These results indicated that interflow was an important hydrological pathway in this purplish soil area. The DOC concentration of the surface flow decreased with rainfall duration, with opposite trend for DOC concentration of interflow. The DOC concentrations in the interflow were 1.35–2.34 times higher than those in the surface flow. However, the rainfall intensity and slope had little effect on DOC concentrations in both surface flow and interflow. Furthermore, the DOC loss fluxes via surface flow and interflow were 3.77–26.94 g and 0.41–13.73 g, respectively, and the ratios of interflow DOC loss fluxes to the total DOC loss fluxes gradually decreased with the increase of rainfall intensity and slope. Under R60, DOC loss via interflow was the major DOC loss pathway, accounting for 51.0%-78.4% of the total DOC loss, whereas for R90 and R120, DOC loss via surface accounted for > 90%. Moreover, runoff volume was positively linearly correlated with the corresponding DOC loss fluxes in both the surface flow (R2 = 0.93, P < 0.01) and interflow (R2 = 0.99, P < 0.01). These results contribute to our understanding of the relationship between carbon cycle and soil erosion, and provide a scientific basis to estimate the fluxes of DOC loss and controll carbon loss in the purplish soil area of China.
Multiple Infected Cardiac Myxoma In Young Female Patient complicated with multiple systemic infarctions: Case report and review of literature
Authors: Aldahmashi¹ˈ² Mohammed , Alassal¹ˈ³ Mohammed , Ibrahim Saifeddin , Sharqawi Amr , Elsaily Ayman , Alkholidy¹ Ramzy , Alsayed¹ˈ³ Anhar , Moselehi¹ˈ³ Mohammed , Zayed¹ Mahmoud , Abdelhadi¹ Maher , Fakher¹ Hussien , Shehab Mohammed , Anagera Mohammed , Farag¹ Atef , Shehab¹ Abubaker , Elamin¹ Ahmed , Altaj¹ Ibrahim ,   Khaled Yasin1
Published: September 2024

Abstract

Background: Cardiac myxomas are the most common primary heart tumors, yet their occurrence in young patients, particularly when infected and leading to multiple systemic infarctions, is exceedingly rare. This case highlights the critical need for early diagnosis and intervention.
An 18-year-old female presented with orthopnea, low-grade fever, chest tightness, and pulmonary edema. She had experienced dyspnea, weight loss, recurrent abdominal pain, and chronic anemia over the past six months. Examination revealed congested lungs, sweating, tachycardia, and laboratory findings indicative of inflammation. Echocardiography identified multiple obstructing masses in the left atrium moving into the left ventricle during diastole, causing severe mitral regurgitation. Imaging revealed old infarcts in the brain, liver, and spleen. Blood cultures were positive for Enterococcus faecalis, and empirical antibiotics were initiated.
Urgent surgery was performed, including left atrial myxoma resection, mitral valve replacement with a bioprosthetic valve, left atrial appendage closure, and closure of the interatrial septum. Postoperative echocardiography confirmed normal left ventricular function and a well-functioning bioprosthetic mitral valve. The patient tolerated the procedure well and was discharged on a regimen of oral antibiotics for six weeks.
Discussion: Infected cardiac myxomas, though rare, pose significant risks due to their potential for systemic embolization and severe complications. This case emphasizes the necessity of comprehensive diagnostic evaluation and timely surgical intervention to prevent catastrophic outcomes.
Conclusion: This case underscores the importance of a multidisciplinary approach and early surgical intervention in managing complex cases of infected cardiac myxoma with systemic embolization. Our findings reinforce the need for vigilance in diagnosing and treating such rare but life-threatening conditions.
Long-lasting behavioral, molecular and functional connectivity alterations after chronic exposure to THC in adolescent mice
Authors: Aso Ester , Gomez-Acero Laura , Varriano Federico , Sanchez-Fernandez Nuria , Ciruela Francisco , Soria Guadalupe 
Published: September 2024

Abstract

Heavy and daily use of cannabis with high contents of Δ9-tetrahydrocannabinol (THC) during adolescence is associated with an increased risk of developing psychotic disorders later in life. Here, we have generated a mouse model of THC exposure during adolescence that exhibits impairments in social interaction and increased vulnerability to develop sensorimotor gating deficiencies comparable to those previously described among heavy cannabis consumers. Importantly, we provide evidence on long-term cortico-striatal dysconnectivity induced by exposure to THC during adolescence and its correlation with impaired social interactions occurring later in adulthood. Moreover, we have observed long-lasting molecular alterations in key elements that regulate the mesolimbic dopaminergic system, namely on the balance between dopamine D2, adenosine A2A, and cannabinoid CB1 receptors in the striatum of treated mice. Together, these findings contribute to a better understanding of the neurobiological bases of the deleterious effects associated with cannabis abuse during adolescence and point to the D2R, A2AR and CB1R equilibrium as a potential target to reverse or prevent these effects.
Anesthetic management of a patient with type III spinal muscular atrophy and multinodular goiter undergoing elective thyroidectomy: A case report
Authors: Malekzadeh Shervin , Montaseri Alireza , Eslami Babak , jafari fateme , Rahimi Mojgan 
Published: September 2024

Abstract

Background
Anesthesia management in Spinal Muscular Atrophy (SMA) patients is challenging due to muscle weakness, respiratory complications, and increased sensitivity to muscle relaxants. Additionally, benign multinodular goiter can cause airway obstruction due to tracheal deviation, compression, or extension behind the sternum.
Case presentation:
Here, we report a case of a spinal muscular atrophy type III patient with a huge multinodular goiter who was a candidate for total thyroidectomy. We performed awake nasal fiberoptic intubation with the spray-as-you-go technique without using any muscle relaxant.
Conclusion
Managing anesthesia in neuromuscular patients is challenging. Awake fiberoptic intubation presents a promising technique to avoid muscle relaxants and minimize respiratory complications.
The prognostic value of the neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in colorectal cancer patients experiencing anastomotic leakage：a retrospective study
Authors: Xu Nuo , Huang Zhuo , Mao Lian-Chun , Ma Dan-Dan , Zhang Jian-Xin , Jin Wei-Dong 
Published: September 2024

Abstract

Objective
The purpose of this study was to investigate the influence and predictive value of preoperative peripheral blood NLR and PLR on the prognosis of CAL patients.
Methods.
This study retrospectively analyzed the clinical data of 1016 patients who underwent radical resection for colorectal cancer at a single center between January 1, 2007 and December 31, 2023. In this study, NLR and PLR were analyzed before surgery. Kaplan-Meier survival analysis was performed according to the postoperative survival status of the patients. nomogram and calibration curve were established by Cox proportional risk model to verify its predictive value.
Results
A total of 890 patients with colorectal cancer, 102 patients with CAL, and 788 patients with non-AL colorectal cancer were enrolled for a median follow-up of 96 months (quartile range 33–133). In this study, COX regression analysis showed that preoperative NLR and PLR could predict the prognosis of CAL patients, and the optimal cut-off values of NLR and PLR were 2.89 and 157.62, respectively. Kaplan-Meier survival curve results showed that 5-year OS and DFS in the low NLR and PLR group were significantly higher than those in the high NLR and PLR group. OS and DFS were divided into high, low NLR and PLR groups. Finally, based on COX proportional risk model, a nomogram analysis was conducted to analyze the risk factors affecting OS and DFS, and the accuracy and practicality of the model were verified by calibration curve and decision curve.
Conclusion
Preoperative NLR and PLR can predict the long-term prognosis of CRC and CAL patients, and patients with NLR ≥ 2.89 and PLR ≥ 157.62 have poor survival prognosis. nomogram and calibration curve analysis will further improve the accuracy of OS and DFS prediction.