bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are commercial sweet cherry rootstocks adapted to climate change? Short‐term waterlogging and CO2 effects on sweet cherry cv. ‘Burlat’
Authors: Pérez‐Jiménez Margarita , Hernández‐Munuera María , Piñero M. Carmen , López‐Ortega Gregorio , del Amor Francisco M. 
Published: January 2017

AbstractHigh CO2 is able to ameliorate some negative effects due to climate change and intensify others. This study involves the sweet cherry (Prunus avium) cultivar ‘Burlat’ grafted on the ‘Mariana 2624’, ‘Adara’ and ‘LC 52’ rootstocks. In a climate chamber at two CO2 concentrations, ambient (400 µmol mol−1) and elevated (800 µmol mol−1), the plants were submitted to waterlogging for 7 d, followed by 7 d of recovery after drainage.Waterlogging drastically decreased the rate of photosynthesis, significantly endangering plant survival, particularly for the ‘LC 52’ and ‘Adara’ rootstocks. ‘Mariana 2624’ was also clearly affected by waterlogging that increased lipid peroxidation and the Cl− and SO42− concentrations in all the studied plants. Nevertheless, CO2 was able to overcome this reduction in photosynthesis, augmenting growth, increasing soluble sugars and starch, raising turgor and regulating the concentrations of Cl− and SO42−, while lowering the NO3− concentration in leaves of all the studied rootstocks. In concordance with these results, the proline levels indicated a more intense stress at control CO2 than at high CO2 for waterlogged plants. ‘Mariana 2624’ was more resistant to waterlogging than ‘Adara’, and both were more resistant than ‘LC 52’ in control CO2 conditions; this clearly enhanced the chance of survival under hypoxia.
23‐Hydroxytormentic acid protects human dermal fibroblasts by attenuating UVA‐induced oxidative stress
Authors: Youn Hae Jeong , Kim Ki Bbeum , Han Hyo‐Sun , An In‐Sook , Ahn Kyu Joong 
Published: January 2017

SummaryBackgroundUltraviolet A (UVA), one of the major components of sunlight, can penetrate the dermal layer of the skin and generate reactive oxygen species (ROS). It causes alterations in the dermal connective tissue and gene expression, inflammation, photoaging, and DNA damage.AimsTherefore, the harmful effects of UVA and strategies to reduce it have been consistently investigated. 23‐Hydroxytormentic acid (23‐HTA) has been demonstrated to improve drug‐induced nephrotoxicity and exhibit several free radical scavenging effects with other molecules. Therefore, the aim of this study was to investigate the anti‐inflammatory effects and extracellular matrix (ECM) reconstructive activity of 23‐HTA in UVA‐irradiated normal human dermal fibroblasts (NHDFs).Materials and MethodsThe antioxidant capacity of 23‐HTA was determined by examining its scavenging activities against hydrogen peroxide, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid), and diphenylpicrylhydrazyl in vitro. Its effect on cell viability was evaluated using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tertazolium bromide, and 2,7‐dichlorofluorescin diacetate was used to investigate intracellular ROS scavenging activity. The mRNA levels of antioxidant enzymes and pro‐inflammatory cytokines were detected using quantitative real‐time polymerase chain reaction. A senescence‐associated β‐galactosidase (SA‐β‐gal) staining kit was used to assess senescent cells.Results23‐HTA showed antioxidant capacity mediated by ROS scavenging and regulation of antioxidant‐related gene expression. Further, the SA‐β‐gal analysis and mRNA expression of matrix metalloproteinases and type I procollagen suggested that 23‐HTA regulates the gene expression of ECM proteins and cellular senescence under UVA‐irradiated conditions.ConclusionIn conclusion, 23‐HTA protects against and attenuates UVA‐induced oxidative stress in NHDFs likely via the nuclear factor erythroid‐derived 2‐like 2 signaling pathway.
Microbiomes in respiratory health and disease: An Asia‐Pacific perspective
Authors: Chotirmall Sanjay H. , Gellatly Shaan L. , Budden Kurtis F. , Mac Aogáin Micheál , Shukla Shakti D. , Wood David L.A. , Hugenholtz Philip , Pethe Kevin , Hansbro Philip M. 
Published: January 2017

ABSTRACTThere is currently enormous interest in studying the role of the microbiome in health and disease. Microbiome's role is increasingly being applied to respiratory diseases, in particular COPD, asthma, cystic fibrosis and bronchiectasis. The changes in respiratory microbiomes that occur in these diseases and how they are modified by environmental challenges such as cigarette smoke, air pollution and infection are being elucidated. There is also emerging evidence that gut microbiomes play a role in lung diseases through the modulation of systemic immune responses and can be modified by diet and antibiotic treatment. There are issues that are particular to the Asia‐Pacific region involving diet and prevalence of specific respiratory diseases. Each of these issues is further complicated by the effects of ageing. The challenges now are to elucidate the cause and effect relationships between changes in microbiomes and respiratory diseases and how to translate these into new treatments and clinical care. Here we review the current understanding and progression in these areas.
Spatiotemporal Trends in Teen Birth Rates in the USA, 2003–2012
Authors: Khan Diba , Rossen Lauren M. , Hamilton Brady , Dienes Erin , He Yulei , Wei Rong 
Published: January 2017

Summary
The objective of this analysis was to explore temporal and spatial variation in teen birth rates TBRs across counties in the USA, from 2003 to 2012, by using hierarchical Bayesian models. Prior examination of spatiotemporal variation in TBRs has been limited by the reliance on large-scale geographies such as states, because of the potential instability in TBRs at smaller geographical scales such as counties. We implemented hierarchical Bayesian models with space–time interaction terms and spatially structured and unstructured random effects to produce smoothed county level TBR estimates, allowing for examination of spatiotemporal patterns and trends in TBRs at a smaller geographic scale across the USA. The results may help to highlight US counties where TBRs are higher or lower and to inform efforts to reduce birth rates to adolescents in the USA further.
Upstream control of river anastomosis by sediment overloading, upper Columbia River, British Columbia, Canada
Authors: Makaske Bart , Lavooi Eva , de Haas Tjalling , Kleinhans Maarten G. , Smith Derald G. 
Published: January 2017

AbstractAnastomosing rivers, systems of multiple interconnected channels that enclose floodbasins, constitute a major category of rivers for which various sedimentary facies models have been developed. While the sedimentary products of anastomosing rivers are relatively well‐known, their genesis is still debated. A rapidly growing number of ancient alluvial successions being interpreted as of anastomosing river origin, including important hydrocarbon reservoirs, urge the development of robust models for the genesis of anastomosis, to facilitate better interpretation of ancient depositional settings and controls. The upper Columbia River, British Columbia, Canada, is the most‐studied anastomosing river and has played a key role in the development of an anastomosing river facies model. Two hypotheses for the origin of upper Columbia River anastomosis include the following: (i) downstream control by aggrading cross‐valley alluvial fans; and (ii) upstream control by excessive bedload input from tributaries. Both upstream and downstream control may force aggradation and avulsions in the upper Columbia River. In order to test both hypotheses, long‐term (millennia‐scale) floodplain sedimentation rates and avulsion frequencies are calculated using 14C‐dated deeply buried organic floodplain material from cross‐valley borehole transects. The results indicate a downstream decrease in floodplain sedimentation rate and avulsion frequency along the anastomosed reach, which is consistent with dominant upstream control by sediment overloading. The data here link recent avulsion activity to increased sediment supply during the Little Ice Age (ca 1100 to 1950 ad). This link is supported by data showing that sediment supply to the upper Columbia study reach fluctuated in response to Holocene glacial advances and retreats in the hinterland. Upstream control of anastomosis has considerable implications for the reconstruction of the setting of interpreted ancient anastomosing systems. The present research underscores that anastomosing systems typically occur in relatively proximal settings with abundant sediment supplied to low‐gradient floodplains, a situation commonly found in intermontane and foreland basins.
Zea mays Fe deficiency‐related 4 (ZmFDR4) functions as an iron transporter in the plastids of monocots
Authors: Zhang Xiu‐Yue , Zhang Xi , Zhang Qi , Pan Xiao‐Xi , Yan Luo‐Chen , Ma Xiao‐Juan , Zhao Wei‐Zhong , Qi Xiao‐Ting , Yin Li‐Ping 
Published: January 2017

SummaryIron (Fe)‐homeostasis in the plastids is closely associated with Fe transport proteins that prevent Fe from occurring in its toxic free ionic forms. However, the number of known protein families related to Fe transport in the plastids (about five) and the function of iron in non‐green plastids is limited. In the present study, we report the functional characterization of Zea mays Fe deficiency‐related 4 (ZmFDR4), which was isolated from a differentially expressed clone of a cDNA library of Fe deficiency‐induced maize roots. ZmFDR4 is homologous to the bacterial FliP superfamily, coexisted in both algae and terrestrial plants, and capable of restoring the normal growth of the yeast mutant fet3fet4, which possesses defective Fe uptake systems. ZmFDR4 mRNA is ubiquitous in maize and is inducible by iron deficiency in wheat. Transient expression of the 35S:ZmFDR4–eGFP fusion protein in rice protoplasts indicated that ZmFDR4 maybe localizes to the plastids envelope and thylakoid. In 35S:c‐Myc‐ZmFDR4 transgenic tobacco, immunohistochemistry and immunoblotting confirmed that ZmFDR4 is targeted to both the chloroplast envelope and thylakoid. Meanwhile, ultrastructure analysis indicates that ZmFDR4 promotes the density of plastids and accumulation of starch grains. Moreover, Bathophenanthroline disulfonate (BPDS) colorimetry and inductively coupled plasma mass spectrometry (ICP‐MS) indicate that ZmFDR4 is related to Fe uptake by plastids and increases seed Fe content. Finally, 35S:c‐Myc‐ZmFDR4 transgenic tobacco show enhanced photosynthetic efficiency. Therefore, the results of the present study demonstrate that ZmFDR4 functions as an iron transporter in monocot plastids and provide insight into the process of Fe uptake by plastids.
Implementation of improved wetland systems as a ‘zero‐discharge‐technology’ in Poland
Authors: Halicki W. , Kita K. 
Published: January 2017

AbstractThis paper discusses results from the implementation of a ‘zero‐discharge‐ technology’ primarily for single households, as well as hotels and small residential urban and rural areas. During a 15‐year span, 5600 improved wetland systems (IWS) were built, and approximately 50% of treatment wetlands in Europe are located in Poland. The first section of this paper discusses water and wastewater management and its development trends. Particular attention is paid to creating sustainable water management in rural areas due to increased necessity in these regions. The second section presents the principle of the IWS, which consists of two options. The third section discusses the current requirements regarding the quality of treated wastewater in Poland. The fourth section presents the effectiveness of the implementation of this system by the Institute for Applied Ecology and the further prospects of ‘zero‐discharge‐technology’ development. The last section discusses the obtained results in the reduction of pollution.
Visceral and somatic pain modalities reveal NaV1.7‐independent visceral nociceptive pathways
Authors: Hockley James R. F. , González‐Cano Rafael , McMurray Sheridan , Tejada‐Giraldez Miguel A. , McGuire Cian , Torres Antonio , Wilbrey Anna L. , Cibert‐Goton Vincent , Nieto Francisco R. , Pitcher Thomas , Knowles Charles H. , Baeyens José Manuel , Wood John N. , Winchester Wendy J. , Bulmer David C. , Cendán Cruz Miguel , McMurray Gordon 
Published: January 2017

Key points
Voltage‐gated sodium channels play a fundamental role in determining neuronal excitability.
Specifically, voltage‐gated sodium channel subtype NaV1.7 is required for sensing acute and inflammatory somatic pain in mice and humans but its significance in pain originating from the viscera is unknown.
Using comparative behavioural models evoking somatic and visceral pain pathways, we identify the requirement for NaV1.7 in regulating somatic (noxious heat pain threshold) but not in visceral pain signalling.
These results enable us to better understand the mechanisms underlying the transduction of noxious stimuli from the viscera, suggest that the investigation of pain pathways should be undertaken in a modality‐specific manner and help to direct drug discovery efforts towards novel visceral analgesics.
AbstractVoltage‐gated sodium channel NaV1.7 is required for acute and inflammatory pain in mice and humans but its significance for visceral pain is unknown. Here we examine the role of NaV1.7 in visceral pain processing and the development of referred hyperalgesia using a conditional nociceptor‐specific NaV1.7 knockout mouse (NaV1.7Nav1.8) and selective small‐molecule NaV1.7 antagonist PF‐5198007. NaV1.7Nav1.8 mice showed normal nociceptive behaviours in response to intracolonic application of either capsaicin or mustard oil, stimuli known to evoke sustained nociceptor activity and sensitization following tissue damage, respectively. Normal responses following induction of cystitis by cyclophosphamide were also observed in both NaV1.7Nav1.8 and littermate controls. Loss, or blockade, of NaV1.7 did not affect afferent responses to noxious mechanical and chemical stimuli in nerve–gut preparations in mouse, or following antagonism of NaV1.7 in resected human appendix stimulated by noxious distending pressures. However, expression analysis of voltage‐gated sodium channel α subunits revealed NaV1.7 mRNA transcripts in nearly all retrogradely labelled colonic neurons, suggesting redundancy in function. By contrast, using comparative somatic behavioural models we identify that genetic deletion of NaV1.7 (in NaV1.8‐expressing neurons) regulates noxious heat pain threshold and that this can be recapitulated by the selective NaV1.7 antagonist PF‐5198007. Our data demonstrate that NaV1.7 (in NaV1.8‐expressing neurons) contributes to defined pain pathways in a modality‐dependent manner, modulating somatic noxious heat pain, but is not required for visceral pain processing, and advocate that pharmacological block of NaV1.7 alone in the viscera may be insufficient in targeting chronic visceral pain.
Draft Genome Sequence of Hydrogenibacillus schlegelii MA48, a Deep-Branching Member of the
Bacilli
Class of
Firmicutes
Authors: Maker Allison , Hemp James , Pace Laura A. , Ward Lewis M. , Fischer Woodward W. 
Published: January 2017

ABSTRACT

We report here the draft genome sequence of
Hydrogenibacillus schlegelii
MA48, a thermophilic facultative anaerobe that can oxidize hydrogen aerobically.
H. schlegelii
MA48 belongs to a deep-branching clade of the
Bacilli
class and provides important insight into the acquisition of aerobic respiration within the
Firmicutes
phylum.
Draft Genome Sequence of an Enterococcus faecalis ATCC 19433 Siphovirus Isolated from Raw Domestic Sewage
Authors: Santiago-Rodriguez Tasha M. , Ly Melissa , Pride David T. , Toranzos Gary A. 
Published: January 2017

ABSTRACT

We previously isolated and characterized an
Enterococcus faecalis
ATCC 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. Here, we report the draft genome sequence of this bacteriophage.
Genome Sequences of Diverse Human Cytomegalovirus Strains with Utility in Drug Screening and Vaccine Evaluation
Authors: Corcoran Kathleen , Sherrod Carly J. , Perkowski Ellen F. , Texier Jordan , Li Fengsheng , Wang I-Ming , McVoy Michael , Fu Tong-Ming , Dittmer Dirk P. 
Published: January 2017

ABSTRACT
Cytomegalovirus displays genetic heterogeneity, which has implications for antiviral and vaccine development. Many studies have focused on laboratory isolates that have been extensively adapted for growth on fibroblasts. Here, we report whole-genome sequences for 10 human cytomegalovirus (HCMV) strains that readily grow on ARPE-19 human retinal pigment epithelial cells.
Draft Genome Sequence of the Oleaginous Green Alga
Tetradesmus obliquus
UTEX 393
Authors: Carreres B. M. , de Jaeger L. , Springer J. , Barbosa M. J. , Breuer G. , van den End E. J. , Kleinegris D. M. M. , Schäffers I. , Wolbert E. J. H. , Zhang H. , Lamers P. P. , Draaisma R. B. , Martins dos Santos V. A. P. , Wijffels R. H. , Eggink G. , Schaap P. J. , Martens D. E. 
Published: January 2017

ABSTRACT

The microalgae
Tetradesmus obliquus
is able to maintain a high photosynthetic efficiency under nitrogen limitation and is considered a promising green microalgae for sustainable production of diverse compounds, including biofuels. Here, we report the first draft whole-genome shotgun sequencing of
T. obliquus
. The final assembly comprises 108,715,903 bp with over 1,368 scaffolds.
First Complete Providencia rettgeri Genome Sequence, the NDM-1-Producing Clinical Strain RB151
Authors: Marquez-Ortiz R. Alejandro , Haggerty Leanne , Sim Eby M. , Duarte Carolina , Castro-Cardozo Betsy E. , Beltran Mauricio , Saavedra Sandra , Vanegas Natasha , Escobar-Perez Javier , Petty Nicola K. 
Published: January 2017

ABSTRACT

Providencia rettgeri
is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. Here, we report the first complete genome sequence of
P. rettgeri
. The genome of strain RB151 consists of a 4.8-Mbp chromosome and a 108-kbp
bla
NDM-1
-positive plasmid.
Draft Genome Sequences of Four Alkaliphilic Bacteria Belonging to the
Anaerobacillus
Genus
Authors: Bassil Naji M. , Lloyd Jonathan R. 
Published: January 2017

ABSTRACT

The draft genomes of the alkaliphilic, anaerobic bacteria,
Anaerobacillus arseniciselenatis
,
A. alkalidiazotrophicus
, and
A. alkalilacustris
, and a novel closely related isolate of the
Anaerobacillus
genus are reported here. These assembled genomes will help identify, at the molecular level, the phenotypic differences between the species of this poorly characterized genus.
Natural course of chronic fatigue syndrome/myalgic encephalomyelitis in adolescents
Authors: Norris Tom , Collin Simon M , Tilling Kate , Nuevo Roberto , Stansfeld Stephen A , Sterne Jonathan AC , Heron Jon , Crawley Esther 
Published: January 2017

ObjectiveLittle is known about persistence of or recovery from chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) in adolescents. Previous studies have small sample sizes, short follow-up or have focused on fatigue rather than CFS/ME or, equivalently, chronic fatigue, which is disabling. This work aimed to describe the epidemiology and natural course of CFS/ME in adolescents aged 13–18 years.DesignLongitudinal follow-up of adolescents enrolled in the Avon Longitudinal Study of Parents and Children.SettingAvon, UK.ParticipantsWe identified adolescents who had disabling fatigue of >6 months duration without a known cause at ages 13, 16 and 18 years. We use the term ‘chronic disabling fatigue’ (CDF) because CFS/ME was not verified by clinical diagnosis. We used multiple imputation to obtain unbiased estimates of prevalence and persistence.ResultsThe estimated prevalence of CDF was 1.47% (95% CI 1.05% to 1.89%) at age 13, 2.22% (1.67% to 2.78%) at age 16 and 2.99% (2.24% to 3.75%) at age 18. Among adolescents with CDF of 6 months duration at 13 years 75.3% (64.0% to 86.6%) were not classified as such at age 16. Similar change was observed between 16 and 18 years (75.0% (62.8% to 87.2%)). Of those with CDF at age 13, 8.02% (0.61% to 15.4%) presented with CDF throughout the duration of adolescence.ConclusionsThe prevalence of CDF lasting 6 months or longer (a proxy for clinically diagnosed CFS/ME) increases from 13 to 18 years. However, persistent CDF is rare in adolescents, with approximately 75% recovering after 2–3 years.
Prevalence and determinants of current and secondhand smoking in Greece: results from the Global Adult Tobacco Survey (GATS) study
Authors: Rachiotis George , Barbouni Anastasia , Katsioulis Antonis , Antoniadou Eleni , Kostikas Konstantinos , Merakou Kyriakoula , Kourea Kallirrhoe , Khoury Rula N , Tsouros Agis , Kremastinou Jenny , Hadjichristodoulou Christos 
Published: January 2017

ObjectivesGreece is one of the leading tobacco-producing countries in European Union, and every year over 19 000 Greeks die from tobacco-attributable diseases. The aim of the present study was to provide nationally representative estimates on current and secondhand smoking prevalence in Greece and their determinants.DesignCross-sectional.SettingGreece.ParticipantsA total of 4359 individuals participated in the Global Adult Tobacco Survey (GATS), a household survey of adults ≥15 years old (overall response rate 69%). They were selected through a multistage geographically clustered sampling design with face-to-face interview.Primary and secondary outcome measuresIn 2013, we investigated the prevalence of current and secondhand smoking and their determinants. Univariate and logistic regression analysis was used in order to identify possible risk factors associated with the prevalence of current and secondhand smoking.ResultsThe prevalence of current smoking was 38.2% (95% CI 35.7% to 40.8%), and the mean number of cigarettes smoked per day was 19.8. Multivariate analysis confirmed that male gender (OR=3.24; 95% CI 2.62 to 4.00), age groups (25–39, OR=4.49; 95% CI 3.09 to 8.46 and 40–54, OR=3.51; 95% CI 1.88 to 5.87) and high school education (OR=1.97; 95% CI 1.41 to 2.74) were independently associated with the current smoking. Remarkably, responders with primary or less education had the lowest prevalence of current smoking (p<0.001). The prevalence of exposure to secondhand smoke at work, home and restaurants, was 52.3%, 65.7% and 72.2%. In total, 90.0% (95% CI 87.8% to 91.9%) of Greek population is exposed to tobacco smoke (current smoking and secondhand smoke).ConclusionsOur results revealed an extremely high prevalence of current smoking and exposure to secondhand smoke among the adult population and a positive gradient between education and current smoking. These findings are alarming and implementation of comprehensive tobacco control and prevention strategies could be impactful in fighting the tobacco epidemic in Greece.