bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of long non-coding RNA-RNCR3 in atherosclerosis-related vascular dysfunction
Authors: Shan K , Jiang Q , Wang X -Q , Wang Y -N -Z , Yang H , Yao M -D , Liu C , Li X -M , Yao J , Liu B , Zhang Y -Y , J Yong , Yan B 
Published: June 2016

AbstractAtherosclerosis is one of the most common vascular disorders. Endothelial cell (EC) dysfunction and vascular smooth muscle cell (VSMC) proliferation contributes to the development of atherosclerosis. Long non-coding RNAs (lncRNAs) have been implicated in several biological processes and human diseases. Here we show that lncRNA-RNCR3 is expressed in ECs and VSMCs. RNCR3 expression is significantly upregulated in mouse and human aortic atherosclerotic lesions, and cultured ECs and VSMCs upon ox-LDL treatment in vitro. RNCR3 knockdown accelerates the development of atherosclerosis, aggravates hypercholesterolemia and inflammatory factor releases, and decreases EC and VSMC proliferation in vivo. RNCR3 knockdown also reduces the proliferation and migration, and accelerates apoptosis development of EC and VSMC in vitro. RNCR3 acts as a ceRNA, and forms a feedback loop with Kruppel-like factor 2 and miR-185-5p to regulate cell function. This study reveals that RNCR3 has an atheroprotective role in atherosclerosis, and its intervention is a promising strategy for treating atherosclerosis-related vascular dysfunction.
Proteolytic degradation and potential role of onconeural protein cdr2 in neurodegeneration
Authors: Hwang J-Y , Lee J , Oh C-K , Kang H W , Hwang I-Y , Um J W , Park H C , Kim S , Shin J-H , Park W-Y , Darnell R B , Um H-D , Chung K C , Kim K , Oh Y J 
Published: June 2016

AbstractCerebellar degeneration-related protein 2 (cdr2) is expressed in the central nervous system, and its ectopic expression in tumor cells of patients with gynecological malignancies elicits immune responses by cdr2-specific autoantibodies and T lymphocytes, leading to neurological symptoms. However, little is known about the regulation and function of cdr2 in neurodegenerative diseases. Because we found that cdr2 is highly expressed in the midbrain, we investigated the role of cdr2 in experimental models of Parkinson’s disease (PD). We found that cdr2 levels were significantly reduced after stereotaxic injection of 1-methyl-4-phenylpyridinium (MPP+) into the striatum. cdr2 levels were also decreased in the brains of post-mortem PD patients. Using primary cultures of mesencephalic neurons and MN9D cells, we confirmed that MPP+ reduces cdr2 in tyrosine hydroxylase-positive dopaminergic neuronal cells. The MPP+-induced decrease of cdr2 was primarily caused by calpain- and ubiquitin proteasome system-mediated degradation, and cotreatment with pharmacological inhibitors of these enzymes or overexpression of calcium-binding protein rendered cells less vulnerable to MPP+-mediated cytotoxicity. Consequently, overexpression of cdr2 rescued cells from MPP+-induced cytotoxicity, whereas knockdown of cdr2 accelerated toxicity. Collectively, our findings provide insights into the novel regulatory mechanism and potentially protective role of onconeural protein during dopaminergic neurodegeneration.
Endoplasmic reticulum stress eIF2α–ATF4 pathway-mediated cyclooxygenase-2 induction regulates cadmium-induced autophagy in kidney
Authors: Luo B , Lin Y , Jiang S , Huang L , Yao H , Zhuang Q , Zhao R , Liu H , He C , Lin Z 
Published: June 2016

AbstractThe heavy metal cadmium (Cd) is nephrotoxic. Recent studies show that autophagy plays an essential role in Cd-induced kidney injury. However, the mechanisms of Cd-induced kidney injury accompanied by autophagy are still obscure. In the present study, we first confirmed that Cd induced kidney damage and dysfunction, along with autophagy, both in vivo and in vitro. Then, we observed that cyclooxygenase-2 (COX-2) and the eIF2α–ATF4 pathway of endoplasmic reticulum (ER) stress were induced by Cd in both kidney tissues and cultured cells. Further studies showed that inhibition of COX-2 with celecoxib or RNA interference (RNAi) inhibited the Cd-induced autophagy in kidney cells. In addition, blocking ER stress with 4-phenylbutyrate or RNAi partially counteracted COX-2 overexpression and autophagy induced by Cd, which suggested that ER stress was required for Cd-induced kidney autophagy. Significantly, our results showed that Cd activated ATF4 and induced its translocation to the nucleus. Knockdown of ATF4 inhibited Cd-induced COX-2 overexpression. While COX-2 overexpression is involved in renal dysfunction, there is no prior report on the role of COX-2 in autophagy regulation. The results of the current study suggest a novel molecular mechanism that the ER stress eIF2α–ATF4 pathway-mediated COX-2 overexpression contributes to Cd-induced kidney autophagy and injury. The present study implies that COX-2 may be a potential target for therapy against Cd-induced nephrotoxicity.
Fast, greener and scalable direct coupling of organolithium compounds with no additional solvents
Authors: Pinxterhuis Erik B. , Giannerini Massimo , Hornillos Valentín , Feringa Ben L. 
Published: June 2016

AbstractAlthough the use of catalytic rather than stoichiometric amounts of metal mediator in cross-coupling reactions between organic halides and organometallic counterparts improves significantly the atom economy and waste production, the use of solvents and stoichiometric generation of main-group byproducts (B, Sn and Zn) hamper the ‘greenness’ and industrial efficiency of these processes. Here we present a highly selective and green Pd-catalysed cross-coupling between organic halides and organolithium reagents proceeding without additional solvents and with short reaction times (10 min). This method bypasses a number of challenges previously encountered in Pd-catalysed cross-coupling with organolithium compounds such as strict exclusion of moisture, dilution and slow addition. Operational ease of this protocol combines the use of industrially viable catalysts loadings (down to 0.1 mol%), scalability of the process (tested up to 120 mmol) and exceptionally favourable environmental impact (E factors in several cases as low as 1).
Shifting El Niño inhibits summer Arctic warming and Arctic sea-ice melting over the Canada Basin
Authors: Hu Chundi , Yang Song , Wu Qigang , Li Zhenning , Chen Junwen , Deng Kaiqiang , Zhang Tuantuan , Zhang Chengyang 
Published: June 2016

AbstractArctic climate changes include not only changes in trends and mean states but also strong interannual variations in various fields. Although it is known that tropical-extratropical teleconnection is sensitive to changes in flavours of El Niño, whether Arctic climate variability is linked to El Niño, in particular on interannual timescale, remains unclear. Here we demonstrate for the first time a long-range linkage between central Pacific (CP) El Niño and summer Arctic climate. Observations show that the CP warming related to CP El Niño events deepens the tropospheric Arctic polar vortex and strengthens the circumpolar westerly wind, thereby contributing to inhibiting summer Arctic warming and sea-ice melting. Atmospheric model experiments can generally capture the observed responses of Arctic circulation and robust surface cooling to CP El Niño forcing. We suggest that identification of the equator-Arctic teleconnection, via the ‘atmospheric bridge’, can potentially contribute to improving the skill of predicting Arctic climate.
ZBTB7A mutations in acute myeloid leukaemia with t(8;21) translocation
Authors: Hartmann Luise , Dutta Sayantanee , Opatz Sabrina , Vosberg Sebastian , Reiter Katrin , Leubolt Georg , Metzeler Klaus H. , Herold Tobias , Bamopoulos Stefanos A. , Bräundl Kathrin , Zellmeier Evelyn , Ksienzyk Bianka , Konstandin Nikola P. , Schneider Stephanie , Hopfner Karl-Peter , Graf Alexander , Krebs Stefan , Blum Helmut , Middeke Jan Moritz , Stölzel Friedrich , Thiede Christian , Wolf Stephan , Bohlander Stefan K. , Preiss Caroline , Chen-Wichmann Linping , Wichmann Christian , Sauerland Maria Cristina , Büchner Thomas , Berdel Wolfgang E. , Wörmann Bernhard J. , Braess Jan , Hiddemann Wolfgang , Spiekermann Karsten , Greif Philipp A. 
Published: June 2016

AbstractThe t(8;21) translocation is one of the most frequent cytogenetic abnormalities in acute myeloid leukaemia (AML) and results in the RUNX1/RUNX1T1 rearrangement. Despite the causative role of the RUNX1/RUNX1T1 fusion gene in leukaemia initiation, additional genetic lesions are required for disease development. Here we identify recurring ZBTB7A mutations in 23% (13/56) of AML t(8;21) patients, including missense and truncating mutations resulting in alteration or loss of the C-terminal zinc-finger domain of ZBTB7A. The transcription factor ZBTB7A is important for haematopoietic lineage fate decisions and for regulation of glycolysis. On a functional level, we show that ZBTB7A mutations disrupt the transcriptional repressor potential and the anti-proliferative effect of ZBTB7A. The specific association of ZBTB7A mutations with t(8;21) rearranged AML points towards leukaemogenic cooperativity between mutant ZBTB7A and the RUNX1/RUNX1T1 fusion.
The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts
Authors: Liu Zhanjiang , Liu Shikai , Yao Jun , Bao Lisui , Zhang Jiaren , Li Yun , Jiang Chen , Sun Luyang , Wang Ruijia , Zhang Yu , Zhou Tao , Zeng Qifan , Fu Qiang , Gao Sen , Li Ning , Koren Sergey , Jiang Yanliang , Zimin Aleksey , Xu Peng , Phillippy Adam M. , Geng Xin , Song Lin , Sun Fanyue , Li Chao , Wang Xiaozhu , Chen Ailu , Jin Yulin , Yuan Zihao , Yang Yujia , Tan Suxu , Peatman Eric , Lu Jianguo , Qin Zhenkui , Dunham Rex , Li Zhaoxia , Sonstegard Tad , Feng Jianbin , Danzmann Roy G. , Schroeder Steven , Scheffler Brian , Duke Mary V. , Ballard Linda , Kucuktas Huseyin , Kaltenboeck Ludmilla , Liu Haixia , Armbruster Jonathan , Xie Yangjie , Kirby Mona L. , Tian Yi , Flanagan Mary Elizabeth , Mu Weijie , Waldbieser Geoffrey C. 
Published: June 2016

AbstractCatfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance.
Giant Seebeck effect in Ge-doped SnSe
Authors: Gharsallah M. , Serrano-Sánchez F. , Nemes N. M. , Mompeán F. J. , Martínez J. L. , Fernández-Díaz M. T. , Elhalouani F. , Alonso J. A. 
Published: June 2016

AbstractThermoelectric materials may contribute in the near future as new alternative sources of sustainable energy. Unprecedented thermoelectric properties in p-type SnSe single crystals have been recently reported, accompanied by extremely low thermal conductivity in polycrystalline samples. In order to enhance thermoelectric efficiency through proper tuning of this material we report a full structural characterization and evaluation of the thermoelectric properties of novel Ge-doped SnSe prepared by a straightforward arc-melting method, which yields nanostructured polycrystalline samples. Ge does not dope the system in the sense of donating carriers, yet the electrical properties show a semiconductor behavior with resistivity values higher than that of the parent compound, as a consequence of nanostructuration, whereas the Seebeck coefficient is higher and thermal conductivity lower, favorable to a better ZT figure of merit.
Wide angle and narrow-band asymmetric absorption in visible and near-infrared regime through lossy Bragg stacks
Authors: Shu Shiwei , Zhan Yawen , Lee Chris , Lu Jian , Li Yang Yang 
Published: June 2016

AbstractAbsorber is an important component in various optical devices. Here we report a novel type of asymmetric absorber in the visible and near-infrared spectrum which is based on lossy Bragg stacks. The lossy Bragg stacks can achieve near-perfect absorption at one side and high reflection at the other within the narrow bands (several nm) of resonance wavelengths, whereas display almost identical absorption/reflection responses for the rest of the spectrum. Meanwhile, this interesting wavelength-selective asymmetric absorption behavior persists for wide angles, does not depend on polarization and can be ascribed to the lossy characteristics of the Bragg stacks. Moreover, interesting Fano resonance with easily tailorable peak profiles can be realized using the lossy Bragg stacks.
miR-146a is essential for lipopolysaccharide (LPS)-induced cross-tolerance against kidney ischemia/reperfusion injury in mice
Authors: Dai Yan , Jia Ping , Fang Yi , Liu Hong , Jiao Xiaoyan , He John C. , Ding Xiaoqiang 
Published: June 2016

AbstractMicroRNA-146a is one of most important microRNAs involved in development of endotoxin tolerance via (toll-like receptors) TLRs/ NF-κB pathway. In this study, we sought to identify the mechanistic role of miR-146a in mediating the protective effect of lipopolysaccharide (LPS) pretreatment on kidney ischemia/reperfusion injury. A locked nucleic acid–modified anti-miR-146a given before LPS treatment knocked down miR-146a expression and completely negated LPS-mediated protection against kidney ischemia/reperfusion injury. Knockdown of miR-146a resulted in significantly higher histopathological scores for tubular damage, expression of proinflammatory cytokines and chemokines and neutrophil and macrophage infiltration. Furthermore, knockdown of miR-146a greatly up-regulated the protein levels of IL-1 receptor-associated kinase (IRAK-1) and tumor-necrosis factor (TNF) receptor-associated factor 6 (TRAF6), which are known target genes of miR-146a, leading to activation of NF-κB. Finally, elevation of nuclear translocation of NF-κB p65/p50 and caspase-3 expression, degradation of cytosolic IkBα and BcL-xL and substantially exacerbation of tubular cell apoptosis were inversely correlated with miR-146a expression. Taken together, our results identify that miR146a exerts a kidney protective effect through negative regulation of acute inflammatory response by suppressing NF-κB activation and proinflammatory genes expression.
Proteomic Response and Quality Maintenance in Postharvest Fruit of Strawberry (Fragaria × ananassa) to Exogenous Cytokinin
Authors: Li Li , Li Dongdong , Luo Zisheng , Huang Xinhong , Li Xihong 
Published: June 2016

AbstractThe limitations in current understanding of the molecular mechanisms underlying fruit response to the application of plant growth regulators have increasingly become major challenges in improvement of crop quality. This study aimed to evaluate the response of strawberry to the preharvest application of exogenous cytokinin known as forchlorfenuron (CPPU). Postharvest internal and physiological quality attributes were characterized following storage under different conditions. Hierarchical clustering analysis via a label-free proteomic quantitative approach identified a total of 124 proteins in strawberries across all treatments. The expression profiles of both proteins and genes spanned the ranged role of cytokinin involved in primary and secondary metabolism, stress response and so on. Eighty-eight proteins and fifty-six proteins were significantly regulated immediately at harvest and after storage, respectively. In general, the glycolysis in strawberry was only regulated by CPPU before storage; in addition to the accelerated photosynthesis and acid metabolism, CPPU application maintained higher capacity of resistance in strawberry to stress stimuli after storage, in comparison to control. Nevertheless, the volatile biosynthesis in strawberry has been suppressed by exogenous CPPU. Novel cytokinin response proteins and processes were identified in addition to the main transcriptomic expression to gain insights into the phytohormone control of fruit postharvest quality.
Tunable ultraviolet and blue light generation from Nd:YAB random laser bolstered by second-order nonlinear processes
Authors: Moura André L. , Carreño Sandra J. M. , Pincheira Pablo I. R. , Fabris Zanine V. , Maia Lauro J. Q. , Gomes Anderson S. L. , de Araújo Cid B. 
Published: June 2016

AbstractUltraviolet and blue light were obtained by nonlinear frequency conversion in a random laser (RL) based on Nd0.10Y0.90Al3(BO3)4 nanocrystalline powder. RL operation at 1062 nm, due to the 4F3/2 → 4I11/2 transition of neodymium ions (Nd3+), was achieved by exciting the Nd3+ with a tunable beam from 680 to 920 nm covering the ground state absorption transitions to the 4F9/2, (4F7/2,4S3/2), (4F5/2,2H9/2) and 4F3/2 states. Light from 340 to 460 nm was obtained via the second-harmonic generation of the excitation beam while tunable blue light, from 417 to 486 nm, was generated by self-sum-frequency mixing between the excitation beam and the RL emission.
Concurrent enhancement of percolation and synchronization in adaptive networks
Authors: Eom Young-Ho , Boccaletti Stefano , Caldarelli Guido 
Published: June 2016

AbstractCo-evolutionary adaptive mechanisms are not only ubiquitous in nature, but also beneficial for the functioning of a variety of systems. We here consider an adaptive network of oscillators with a stochastic, fitness-based, rule of connectivity and show that it self-organizes from fragmented and incoherent states to connected and synchronized ones. The synchronization and percolation are associated to abrupt transitions and they are concurrently (and significantly) enhanced as compared to the non-adaptive case. Finally we provide evidence that only partial adaptation is sufficient to determine these enhancements. Our study, therefore, indicates that inclusion of simple adaptive mechanisms can efficiently describe some emergent features of networked systems’ collective behaviors and suggests also self-organized ways to control synchronization and percolation in natural and social systems.
Altered resting-state functional activity in posttraumatic stress disorder: A quantitative meta-analysis
Authors: Wang Ting , Liu Jia , Zhang Junran , Zhan Wang , Li Lei , Wu Min , Huang Hua , Zhu Hongyan , Kemp Graham J. , Gong Qiyong 
Published: June 2016

AbstractMany functional neuroimaging studies have reported differential patterns of spontaneous brain activity in posttraumatic stress disorder (PTSD), but the findings are inconsistent and have not so far been quantitatively reviewed. The present study set out to determine consistent, specific regional brain activity alterations in PTSD, using the Effect Size Signed Differential Mapping technique to conduct a quantitative meta-analysis of resting-state functional neuroimaging studies of PTSD that used either a non-trauma (NTC) or a trauma-exposed (TEC) comparison control group. Fifteen functional neuroimaging studies were included, comparing 286 PTSDs, 203 TECs and 155 NTCs. Compared with NTC, PTSD patients showed hyperactivity in the right anterior insula and bilateral cerebellum, and hypoactivity in the dorsal medial prefrontal cortex (mPFC); compared with TEC, PTSD showed hyperactivity in the ventral mPFC. The pooled meta-analysis showed hypoactivity in the posterior insula, superior temporal, and Heschl’s gyrus in PTSD. Additionally, subgroup meta-analysis (non-medicated subjects vs. NTC) identified abnormal activation in the prefrontal-limbic system. In meta-regression analyses, mean illness duration was positively associated with activity in the right cerebellum (PTSD vs. NTC), and illness severity was negatively associated with activity in the right lingual gyrus (PTSD vs. TEC).
Energy transfer from an individual silica nanoparticle to graphene quantum dots and resulting enhancement of photodetector responsivity
Authors: Kim Sung , Shin Dong Hee , Kim Jungkil , Jang Chan Wook , Kang Soo Seok , Kim Jong Min , Kim Ju Hwan , Lee Dae Hun , Kim Jung Hyun , Choi Suk-Ho , Hwang Sung Won 
Published: June 2016

AbstractFörster resonance energy transfer (FRET), referred to as the transfer of the photon energy absorbed in donor to acceptor, has received much attention as an important physical phenomenon for its potential applications in optoelectronic devices as well as for the understanding of some biological systems. If one-atom-thick graphene is used for donor or acceptor, it can minimize the separation between donor and acceptor, thereby maximizing the FRET efficiency (EFRET). Here, we report first fabrication of a FRET system composed of silica nanoparticles (SNPs) and graphene quantum dots (GQDs) as donors and acceptors, respectively. The FRET from SNPs to GQDs with an EFRET of ∼78% is demonstrated from excitation-dependent photoluminescence spectra and decay curves. The photodetector (PD) responsivity (R) of the FRET system at 532 nm is enhanced by 100∼101/102∼103 times under forward/reverse biases, respectively, compared to the PD containing solely GQDs. This remarkable enhancement is understood by network-like current paths formed by the GQDs on the SNPs and easy transfer of the carriers generated from the SNPs into the GQDs due to their close attachment. The R is 2∼3 times further enhanced at 325 nm by the FRET effect.
Thiazolides Elicit Anti-Viral Innate Immunity and Reduce HIV Replication
Authors: Trabattoni Daria , Gnudi Federica , Ibba Salomè V. , Saulle Irma , Agostini Simone , Masetti Michela , Biasin Mara , Rossignol Jean-Francois , Clerici Mario 
Published: June 2016

AbstractNitazoxanide (Alinia®, NTZ) and tizoxanide (TIZ), its active circulating metabolite, belong to a class of agents known as thiazolides (TZD) endowed with broad anti-infective activities. TIZ and RM-4848, the active metabolite of RM-5038, were shown to stimulate innate immunity in vitro. Because natural resistance to HIV-1 infection in HIV-exposed seronegative (HESN) individuals is suggested to be associated with strong innate immune responses, we verified whether TIZ and RM-4848 could reduce the in vitro infectiousness of HIV-1. Peripheral blood mononuclear cells (PBMCs) from 20 healthy donors were infected in vitro with HIV-1BaL in the presence/absence of TIZ or RM4848. HIV-1 p24 were measured at different timepoints. The immunomodulatory abilities of TZD were evaluated by the expression of type I IFN pathway genes and the production of cytokines and chemokines. TZD drastically inhibited in vitro HIV-1 replication (>87%). This was associated with the activation of innate immune responses and with the up-regulation of several interferon-stimulated genes (ISGs), including those involved in cholesterol pathway, particularly the cholesterol-25 hydroxylase (CH25H). TZD inhibition of HIV-1 replication in vitro could be due to their ability to stimulate potent and multifaceted antiviral immune responses. These data warrant the exploration of TZD as preventive/therapeutic agent in HIV infection.