bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mayfly Neocloeon triangulifer senses decreasing oxygen availability (PO2) and responds by reducing ion uptake and altering gene expression
Authors: Cochran Jamie K. , Buchwalter David B. 
Published: October 2024

ABSTRACT
Oxygen availability is central to the energetic budget of aquatic animals and may vary naturally and/or in response to anthropogenic activities. Yet, we know little about how oxygen availability is linked to fundamental processes such as ion transport in aquatic insects. We hypothesized and observed that ion (22Na and 35SO4) uptake would be significantly decreased at O2 partial pressures below the mean critical level (Pcrit, 5.4 kPa) where metabolic rate (ṀO2) is compromised and ATP production is limited. However, we were surprised to observe marked reductions in ion uptake at oxygen partial pressures well above Pcrit, where ṀO2 was stable. For example, SO4 uptake decreased by 51% at 11.7 kPa and 82% at Pcrit (5.4 kPa) while Na uptake decreased by 19% at 11.7 kPa and 60% at Pcrit. Nymphs held for longer time periods at reduced PO2 exhibited stronger reductions in ion uptake rates. Fluids from whole-body homogenates exhibited a 29% decrease in osmolality in the most hypoxic condition. The differential expression of atypical guanylate cyclase (gcy-88e) in response to changing PO2 conditions provides evidence for its potential role as an oxygen sensor. Several ion transport genes (e.g. chloride channel and sodium-potassium ATPase) and hypoxia-associated genes (e.g. ldh and egl-9) were also impacted by decreased oxygen availability. Together, the results of our work suggest that N. triangulifer can sense decreased oxygen availability and perhaps conserves energy accordingly, even when ṀO2 is not impacted.
Identification of genetic risk variants for Type-2 Diabetes mellitus in Pakistani Pashtun population: A case-control association study
Authors: Jan Asif , Mothana Ramzi A. , Jun-Ya Kaimori  , Tahir Muhammad  , Mehtab Khan  , Naveed Rahman  , Abdullah R. Alanzi  
Published: October 2024

Background and Objective: Pakistan, a South Asian developing country, is experiencing a rapid increase in number of diabetes cases. High prevalence ratio of diabetes in Pakistani population and lack of genetic research studies prompted us to design this study. This present study investigated Pakistani Pashtun population for (known and novel SNPs) and its possible correlation with Type-2 Diabetes Mellitus (T2DM).
Methods: This two stage (discovery & validation stage), case-control association study included one thousand individuals (Patients with T2DM=500 & controls=500) from eight districts of Khyber Pakhtunkhwa Pakistan. The study duration/period was from March 2018 to January 2020. In the first stage (the discovery stage) the target population was screened for known and novel T2DM-associated genetic markers. In the validation stage, identified variants were confirmed for T2DM association using MassARRAY genotyping and association analysis.
Results: Exome sequencing detected eleven known and four novel/new genetic markers in the study population. Novel variants were preferred over the known for follow-up analysis/validation. Among the identified variants strong associations were confirmed for the following variants; rs1781133/ANKRD65 (OR=2.10, 95%Cl=1.06–3.08, P=0.003) rs2274791/TTLL10 (OR=1.97, 95%Cl=1.36-2.62, P=0.025), rs71628928/RNF223 (OR=1.82, 95%Cl=0.97-1.92, P=0.041), and rs609805/SCNN1D (OR=2.21, 95%Cl=1.92-3.09, P=0.001) with T2DM; other reported variants showed no noticeable association (having P>0.05) with T2DM.
Conclusion: This study reports new genetic risk variants for T2DM in Pashtun population providing valuable insights into the genetic basis of T2DM in this group.
doi: https://doi.org/10.12669/pjms.40.10.10292
How to cite this: Jan A, Mothana RA, Kaimori JY, Muhammad T, Khan M, Ali SS, et al. Identification of genetic risk variants for Type-2 Diabetes mellitus in Pakistani Pashtun population: A case-control association study. Pak J Med Sci. 2024;40(10):2336-2343. doi: https://doi.org/10.12669/pjms.40.10.10292
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Construction of a disulfidptosis-associated lncRNAs risk model to predict prognosis and immuno-infiltration analysis of lung adenocarcinoma
Authors: Liu Jiaming , Hao Nie , Du Wenji , Song Wei 
Published: October 2024

Objective: To develop a risk model based on LncRNAs associated with disulfidptosis to forecast the prognosis and assess immune infiltration of Lung adenocarcinoma (LUAD).
Methods: This study employed a bioinformatics approach. The study was conducted from March 29, 2023 and concluded on July 1, 2023 at Guangzhou University of Chinese Medicine, Guangzhou, China. Transcriptomic data specific to LUAD were collected from TCGA database. Disulfidptosis-related LncRNAs were preliminarily screened using co-expression analysis, followed by screening using Lasso regression and Cox regression to identify LncRNAs. Subsequently, prognostic prediction models were constructed. To assess the model, survival analysis, subject operating characteristic curves, and calibration curves were employed. To evaluate the tumor microenvironment, the “estimate” package was used, while the “ggpubr” package was utilized to visualize the variations. Additionally, we employed CIBERSORT to examine immune cell infiltration abundance.
Results: A prognostic prediction model was constructed using five LncRNAs. The high-risk group displayed a shorter overall survival and progression-free survival (P<0.05). The concordance index was calculated as 0.704 (95% CI: 0.654-0.754). GSEA analysis reveals that high risk group is associated with the cell cycle pathway and steroid hormone biosynthesis pathway, while the low-risk group is associated with hematopoietic cell pathway and allograft rejection pathway. Immune cell infiltration analysis indicated associations between the prognostic model and activated T cells CD4 memory, T cells CD8, etc.
Conclusions: The risk model of Disulfidptosis-related LncRNAs can predict the prognosis of LUAD and evaluate the immune infiltration, providing a new direction for the treatment of LUAD.
doi: https://doi.org/10.12669/pjms.40.10.9025
How to cite this: Liu J, Nie H, Du W, Song W. Construction of a disulfidptosis-associated lncRNAs risk model to predict prognosis and immuno-infiltration analysis of lung adenocarcinoma. Pak J Med Sci. 2024;40(10):2368-2372. doi: https://doi.org/10.12669/pjms.40.10.9025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Efficacy of probiotics combined with enteral nutrition therapy on intestinal flora, digestive tract symptoms and endogenous environment in patients with gastric cancer undergoing chemotherapy
Authors: Yang Lan , Liang Yuzhu , Li Riheng , Chen Yuan , Zhang Xia 
Published: October 2024

Objective: To investigate the effects of probiotics combined with enteral nutrition therapy on intestinal flora, digestive tract symptoms and endogenous environment in patients with gastric cancer undergoing chemotherapy.
Methods: In this retrospective study, eighty patients with gastric cancer undergoing chemotherapy admitted to Affiliated Hospital of Hebei University from January 2021 to September 2023 were included and divided into two groups by random number table method: the enteral nutrition group and the probiotics combined enteral nutrition group (n=40 each group). The differences in the number of intestinal flora, nutritional indicators, immune function, endotoxin, D-lactic acid levels between the two groups, and the digestive tract symptoms of the two groups before and after treatment were compared and recorded.
Results: The levels of serum total protein (TP), prealbumin (PAB), albumin (ALB) and transferrin (TF) in both groups were higher than those before treatment, and those in the probiotics combined with enteral nutrition group were higher than those in the enteral nutrition group (p< 0.05). The levels of immunoglobulin A (lgA), immunoglobulin M (lgM) and immunoglobulin G (lgG) in the two groups were lower than before treatment, and those in the probiotics combined with the enteral nutrition group were lower than those in the enteral nutrition group (p< 0.05).
Conclusion: Probiotics combined with enteral nutrition results in various benefits in the treatment of patients with gastric cancer undergoing chemotherapy, improving immunity, and reducing gastrointestinal symptoms.
doi: https://doi.org/10.12669/pjms.40.10.9032
How to cite this: Yang L, Liang Y, Li R, Chen Y, Zhang X. Efficacy of probiotics combined with enteral nutrition therapy on intestinal flora, digestive tract symptoms and endogenous environment in patients with gastric cancer undergoing chemotherapy. Pak J Med Sci. 2024;40(10):2344-2349. doi: https://doi.org/10.12669/pjms.40.10.9032
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Signaling Quality: How Refund Bonuses Can Overcome Information Asymmetries in Crowdfunding
Authors: Cason Timothy N. , Tabarrok Alex , Zubrickas Robertas 
Published: October 2024

Crowdfunding can suffer from information asymmetry, leaving some investors disappointed with low-quality projects, whereas other high-quality projects remain unfunded. We show that refund bonuses, which provide investors a payment if a fundraising campaign is unsuccessful, can signal project quality and help overcome the market failure in crowdfunding. Because strong projects have a lower risk of bonus payout, entrepreneurs with strong projects are more likely to offer bonuses. This signals high quality to investors, and due to their updated beliefs, this drives investment toward such projects. An experiment provides supporting empirical evidence for the benefits of this signaling solution to the problems of information asymmetry in crowdfunding. This paper was accepted by Joshua Gans, business strategy. Funding: This work was supported by Schmidt Sciences. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2023.03923 .
Working with carer grief in community palliative care nursing
Authors: Iskov Leonie , Andersson-Noorgard Kurt , Fairbrother Greg 
Published: October 2024

Background: Community nurses (CN), both generalist and palliative care-specific, are key to supporting the carer with their experience of grief. Aim: To identify the grief-related needs of carers and the capacity of CNs to provide for those needs. Methods: A convergent parallel mixed-methods study which employed i) a needs questionnaire among carers of palliative care patients who had recently died; ii) a qualitative interview study among carers and iii) a questionnaire among CNs which canvassed their confidence and skills in working with carer grief. Findings: In the carer survey, just one-third of carers reported engagement with CNs regarding prolonged and anticipatory grief. In the CN survey, low confidence and skill regarding supporting carers with their grief was reported. CNs with palliative care training were more confident and knowledgeable than generalist CNs. Conclusions: Despite offering high ratings for CN compassion and overall quality of support during the dying process, grief support was reported by carers to be missing from their experience of CN care. The role of CNs in providing for the grief-related needs of carers is yet to be established.
Unlocking the mentally ill in Indonesia: An empirical study of the effectiveness of a “Bebas Pasung” program in Central Java
Authors: Tyas Tri Hayuning , Good Mary-Jo D. , Pratikno Bambang , Subandi M. A. , Marchira Carla R. , Good Byron J. 
Published: October 2024

Background
Locking or confinement of persons with severe mental illness has been common in Indonesia. In 2010, the Ministry of Health declared a policy that persons who were locked (pasung) should be unlocked or freed (bebas) from confinement and provided mental health services. This study is an empirical evaluation of the effectiveness of one Bebas Pasung program in Indonesia at two-year follow-up.

Methods
From medical records in Soerojo Mental Hospital, Magelang, Central Java, 114 persons with severe mental illness who had been unlocked, treated, and returned to the community from four districts served by the hospital were identified. At two-year follow-up, 62 caregivers were able to be contacted and willing to participate in a study. Data were collected from hospital records about condition of the patient at time of “unlocking” and at discharge, and primary caregivers were interviewed about the previous locking and care of the patient since return, as well as experiences of caregiving.

Results
We provide descriptive data concerning history of illness, reasons for locking, type of confinement, and care of the individual since return. 58% of those unlocked were men, 80% had diagnoses of schizophrenia, and mean age was 35. At follow-up, 24% of this sample had been re-locked; only 44% took medications regularly, including 33% of those re-locked and 47% of those not relocked. A majority cared for themselves, half were partially or fully productive, and the quality of life of family caregivers improved significantly since their family member was unlocked, treated, and returned home.

Conclusions
This program successfully unlocked, treated, and returned to their homes persons with severe mental illness living in pasung or restraints. Findings suggest such unlocking programs need to be linked more closely to community-based mental health and rehabilitation services, maintain care of the patient, and provide a path toward recovery.
Trend prediction and influencing factors of the production comparative advantage of China’s main apple-producing provinces
Authors: Ning Wenping , Zhang Fuhong , Zhang Meiling 
Published: October 2024

Introduction
The apple industry is an essential industry to assist in rural revitalization. However, in recent years, the urbanization, industrialization, globalization and climate change have brought various challenges to the apple industry in China’s main apple-producing provinces. Given this, effectively identifying, enhancing on apple production comparative advantage (APCA) is imperative to safeguard the long-term sustainable development of China’s apple industry. This study aims to explore the evolutionary trends and influencing factors of APCA, and to provide quantitative support for the formulation of scientific and effective apple production policies.

Methods
In this paper, the APCA of China’s eight main apple-producing provinces from 2013 to 2022 was measured by using a aggregate comparative advantage index. The spatio-temporal dynamic evolution characteristics of APCA were revealed by adopted Arc GIS and kernel density estimation method. Second, the transfer probabilities of different types of APCA were predicted by empolyed traditional and spatial Markov chains. Finally, the driving mechanism of APCA is explored with the panel quantile model.

Results
1) The average value of APCA of the main producing provinces increased from 1.330 in 2013 to 1.419 in 2022. 2) The probabilities of provinces with low, primary and middle level of advantage jumping to the next level are 31.58%, 16.67% and 11.76%, respectively. When the spatial lag type is high-level advantage, the probability of stabilization of the low-level advantage decreases from 68.42% to 0.00%. 3) Nonfarm payrolls have the largest dampening effect at the 40% quantile.

Conclusions
1) Temporally, APCA shows a trend of slow growth, ups and downs. Spatially, APCA shows a distribution pattern of “west high, east low”. 2) APCA mainly shifted sequentially between neighbouring ranks. Besides, the change of APCA had significant spatial spillover effect, and highly advantage provinces featured more prominent proactive spillovers. 3) There is significant heterogeneity among the influencing factors.
The impact of emotion regulation on the establishment of the therapeutic alliance in post-divorce group intervention: A multilevel approach
Authors: Alvarez Irati , Herrero Marta , Martínez-Pampliega Ana 
Published: October 2024

Objective
The present study focuses on the impact of emotion regulation on the establishment of the therapeutic alliance in a context of post-divorce group intervention.

Method
The study involved 177 divorced parents and 60 therapists and was developed through multilevel path analysis.

Results
The data showed an association between emotion regulation and therapeutic alliance across the difficulties of adaptation to divorce, although the results were different from the perspective of the participant and of the therapist and between the individual and the group level.

Conclusion
The study highlights the relevant role of emotion regulation in post-divorce adaptation and in the success of the establishment of the therapeutic alliance, while clarifying the formation of the alliance from the viewpoints of the individual and the therapist. The study also highlights the need to understand the alliance at both the individual and the group level, in order to design therapeutic interventions.
Unusual Confluence: Exploring the Association of Biliary Atresia, Wilson Disease, and Iron Overload
Authors: Duggal Shivangini , Meza-Rodriguez Samantha , Shahid Saqib , Zuckerman Marc , Borges Jorge Chiquie 
Published: October 2024

ABSTRACT
The case involves a 33-year-old man with biliary atresia, Wilson disease (WD), and iron overload. Biliary atresia, a cholangiodestructive disease, leads to cirrhosis if untreated. WD, caused by ATP7B gene mutations, results in copper accumulation affecting the liver and brain. Iron overload can be seen in cases of WD and with hereditary hemochromatosis gene mutations. The patient's concurrent presentation of these conditions poses a unique clinical challenge. Elevated iron levels may worsen WD outcomes. A detailed history and physical examination, genetic testing, and close follow-up are crucial. The case highlights the need for increased awareness and vigilant monitoring of patients with overlapping liver diseases.
Quality control analyses of selected honey samples from Serbia based on their mineral and flavonoid profiles, and the invertase activity
Authors: Arsić Biljana , Kostić Danijela , Mrmošanin Jelena , Zlatanović Ivana , Marković Snežana , Petrović Stefan , Stankov-Jovanović Vesna , Janković Sonja , Mišić Ivana Rašić , Mitić Milan , Pavlović Aleksandra , Tošić Snežana , Georgijev Aneta , Stojanović Gordana 
Published: October 2024

Abstract
Nowadays, climate changes induce deviations in the composition of honey varieties characteristic of a specific geographic region. Therefore, according to Codex Alimentarius for honey, the slight variations in values of determined parameters cannot be strictly regarded as a sign of its adulteration. Here, modified methods are presented for preparing honey samples for inductively coupled plasma-optical emission spectroscopy (ICP-OES) analysis and the high-performance liquid chromatography (HPLC) method to use them as fast and reliable methods in the food industry. ICP-OES analysis suggested that all analysed honey samples are natural, and HPLC-DAD (diode-arrray detection) analysis on the flavonoids showed that one sample is probably not natural. Invertase activity showed an overlap with this HPLC-DAD finding but suggested more samples as possibly adulterated, which can be neglected due to possible thermal treatment of those natural samples, causing a decrease in invertase activity. Therefore, results obtained from the analyses of investigated honey samples based on the mineral content, number, and quantity of flavonoids, together with the invertase activity, indicated that the combination of analyses could be a reliable tool for determining the quality of honey samples.
Intraoperative changes in electrophysiological monitoring can be used to predict clinical outcomes in patients with spinal cavernous malformation
Authors: Li Xiaoyu , Zhang Hongqi , Ren Jian 
Published: October 2024

Abstract

Aim
The study aimed to evaluate the sensitivity and specificity of these monitoring parameters in predicting postoperative neurological dysfunction.

Methods
In this study, a total of 85 patients with spinal cavernous malformations (SCMs) treated at Xuanwu Hospital, Capital Medical University, from November 2012 to August 2017 were included. During the surgical procedures, all patients underwent monitoring of motor evoked potentials (MEP) and somatosensory evoked potentials (SEP). The criteria for warning included a reduction of ≥80% in MEP amplitude and ≥50% in SEP amplitude.

Results
Among 85 patients, 40 (47.1%) had SCMs located in the thoracic segment, 35 (41.2%) in the cervical segment, 6 (7.1%) in the cervical thoracic segment, and 4 (4.7%) in the lumbar segment. MEP recordings were obtained from 81 patients, and the preoperative McCormick score was 1.53 ± 0.69. The sensitivity of multimodal monitoring combined with the criteria of 80% reduction in MEP amplitude and SEP was 83.9%, with a specificity of 69%, a positive predictive value of 69%, and a negative predictive value of 90.4%.

Conclusion
This study emphasizes the crucial role of electrophysiological monitoring, particularly MEP and SEP, during the surgical resection of SCMs. The findings demonstrate that this approach is effective in predicting and preventing postoperative neurological dysfunction, thereby improving patient outcomes.
Thinking from the Home: Emanuele Coccia on Domesticity and Happiness
Authors: Edwards Evan 
Published: October 2024

Abstract
This article traces the idea of domesticity through the work of Emanuele Coccia to explain his conception of happiness. Coccia argues that it is only by taking domestic concerns seriously that philosophy can begin to theorize the possibility of happiness today, claiming that rather than beginning with the polis, “philosophy must think the city from the home, and the home from the kitchen.” The article begins by situating Coccia's work in the tradition of Giorgio Agamben's critical project and then explores the idea of the home from his 2016’s The Life of Plants through 2020’s Metamorphoses to understand the reason for devoting an entire book to the topic in 2021’s Philosophy of the Home. The final sections of this article turn to Philosophy of the Home itself to explain his conception of happiness more concretely, focusing on his conceptions of love and interdependency, as well as the fundamental importance of nourishment and the kitchen.