bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurocognitive subtypes in patients with bipolar disorder and their unaffected siblings
Authors: Russo M. , Van Rheenen T. E. , Shanahan M. , Mahon K. , Perez-Rodriguez M. M. , Cuesta-Diaz A. , Larsen E. , Malhotra A. K. , Burdick K. E 
Published: June 2017

BackgroundOur previous work revealed substantial heterogeneity in the cognitive profile of bipolar disorder (BD) due to the presence of three underlying cognitive subgroups characterized as: globally impaired, selectively impaired, or cognitively intact. In an effort to determine whether these subgroups are differentially related to genetic risk for the illness, we investigated whether cognitive deficits were more pronounced in unaffected siblings (UAS) of BD probands within identified clusters.MethodsCluster analysis was used to identify cognitive clusters in BD (N = 60). UAS (N = 49) were classified into groups according to their proband sibling's cluster assignment; comparisons were made across all clusters and healthy controls (HCs; N = 71).ResultsThree cognitive clusters in BD emerged: a globally impaired (36.7%), a selectively impaired (30%), and a cognitively intact cluster (33.3%). UAS showed a qualitatively similar pattern to their BD siblings; UAS of the globally impaired BD cluster showed verbal memory and general cognitive impairments relative to HCs. In contrast, UAS of the other two clusters did not differ from HCs.ConclusionsThis study corroborates findings from prior work regarding the presence of cognitive heterogeneity in BD. UAS of subjects in the globally impaired BD cluster presented with a qualitatively similar cognitive profile to their siblings and performed worse than all other BD clusters and UAS groups. This suggests that inherited risk factors may be contributing to cognitive deficits more notably in one subgroup of patients with BD, pointing toward differential causes of cognitive deficits in discrete subgroups of patients with the disorder.
A material turn in International Relations: the 4x4, intervention and resistance
Authors: Mac Ginty Roger 
Published: June 2017

AbstractThis article explores how analysis of material objects offers insights into international intervention and reactions to that intervention. Building on studies that examine the 4x4 as emblematic of intervention, the article argues that the 4x4 can also be seen as an object of resistance and agency. To do so, it uses the case study of 4x4 usage in Darfur and draws on primary data including interviews and a UN security incident database. The article is mindful of the limitations of a ‘material turn’ in the study of International Relations, especially in relation to how it might encourage us to overlook agency and structural power. While finding new materialism arguments largely convincing, the case study encourages a note of caution and proposes the notion of ‘materialism+’, which allows for the further investigation of the human/non-human interface, but is circumspect about tendencies towards neophilia, dematerialism, and posthumanism.
ppGpp and cytotoxicity diversity in Shiga toxin-producingEscherichia coli(STEC) isolates
Authors: STELLA A. E. , LUZ D. , PIAZZA R. M. F. , SPIRA B. 
Published: June 2017

SUMMARYShiga toxin-producingEscherichia coli(STEC) is a known food pathogen, which main reservoir is the intestine of ruminants. The abundance of different STEC lineages in nature reflect a heterogeneity that is characterised by the differential expression of certain genotypic characteristics, which in turn are influenced by the environmental conditions to which the microorganism is exposed. Bacterial homeostasis and stress response are under the control of the alarmone guanosine tetraphosphate (ppGpp), which intrinsic levels varies across theE. colispecies. In the present study, 50 STEC isolates from healthy sheep were evaluated regarding their ppGpp content, cytotoxicity and other relevant genetic and phenotypic characteristics. We found that the level of ppGpp and cytotoxicity varied considerably among the examined strains. Isolates that harboured thestx2 gene were the least cytotoxic and presented the highest levels of ppGpp. Allstx2 isolates belonged to phylogroup A, while strains that carriedstx1 or bothstx1 andstx2 genes pertained to phylogroup B1. All but twostx2 isolates belonged to thestx2b subtype. Strains that belonged to phylogroup B1 displayed on average low levels of ppGpp and high cytotoxicity. Overall, there was a negative correlation between cytotoxicity and ppGpp.
Folate status in women of childbearing age with obesity: a review
Authors: Maffoni Silvia , De Giuseppe Rachele , Stanford Fatima Cody , Cena Hellas 
Published: June 2017

AbstractSeveral studies have described a positive association between elevated BMI and birth defects risk. Data on plasma concentration of folate in pregnant women with obesity have shown values far below those recommended, regardless of diet, while folate levels should increase before pregnancy to reduce neural tube defects. We report a descriptive review of the most recent studies (from 2005 to 2015) to evaluate folate status through a population of women of childbearing age affected by obesity. The literature contains few studies, which present conflicting results regarding folate status in non-pregnant women of childbearing age affected by obesity, and it appears that there is a modification in folate metabolism, with a reduction in plasma folate levels and an increase in erythrocyte folate uptake. In conclusion, the folate status in women of childbearing age should be assessed by both plasma and erythrocyte levels to start a personalised and more adequate supplementation before conception. Further studies need to be conducted in a larger population, which take into account variables that can affect folate metabolism, such as dietary intake, lifestyle and genetic factors, oral contraceptives or other drug use, previous weight-loss programmes, or a history of bariatric surgery.
Accurate diagnosis of CHD by Paediatricians with Expertise in Cardiology
Authors: Jacob Hannah C. , Massey Hannah , Yates Robert W. M. , Kelsall A. Wilfred 
Published: June 2017

AbstractIntroductionPaediatricians with Expertise in Cardiology assess children with a full history, examination, and often perform an echocardiogram. A minority are then referred to an outreach clinic run jointly with a visiting paediatric cardiologist. The accuracy of the echocardiography diagnosis made by the Paediatrician with Expertise in Cardiology is unknown.Materials and methodsWe conducted a retrospective review of clinic letters for children seen in the outreach clinic for the first time between March, 2004 and March, 2011. Children with CHD diagnosed antenatally or elsewhere were excluded. We recorded the echocardiography diagnosis made by the paediatric cardiologist and previously by the Paediatrician with Expertise in Cardiology.ResultsThe Paediatrician with Expertise in Cardiology referred 317/3145 (10%) children seen in the local cardiac clinics to the outreach clinic over this period, and among them 296 were eligible for inclusion. Their median age was 1.5 years (range 1 month–15.1 years). For 244 (82%) children, there was complete diagnostic agreement between the Paediatrician with Expertise in Cardiology and the paediatric cardiologist. For 29 (10%) children, the main diagnosis was identical with additional findings made by the paediatric cardiologist. The abnormality had resolved in 17 (6%) cases by the time of clinic attendance. In six (2%) patients, the paediatric cardiologist made a different diagnosis. In total, 138 (47%) patients underwent a surgical or catheter intervention.DiscussionPaediatricians with Expertise in Cardiology can make accurate diagnoses of CHD in children referred to their clinics. This can allow effective triage of children attending the outreach clinic, making best use of limited specialist resources.
‘So very unequal to the place’? The Legal Apprenticeship of John Williams, Lord Keeper, c. 1605–1621
Authors: Nicholls Andrew D. 
Published: June 2017

AbstractIn the spring of 1621, James I sent the Great Seal of England to his newly appointed Lord Keeper, John Williams, the Dean of Westminster. Naming an ecclesiastic to this position shocked contemporary legal and political commentators, and subsequent historians have generally shared this negative appraisal. Even more positive analyses have held that Williams’ primary attraction for the king lay in his intellect and learning, and an expectation that he would do James’ bidding on the Court of Chancery. Williams actually possessed both stronger legal qualifications than have traditionally been recognized, and a politico-legal philosophy that had helped to modify James’ own views of the role of his prerogative courts and powers. An examination of Williams’ career prior to 1621 reveals the development of a candidate uniquely placed to fill this particular role at that specific moment in James’ reign.
The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple
Authors: An Jian-Ping , Qu Feng-Jia , Yao Ji-Fang , Wang Xiao-Na , You Chun-Xiang , Wang Xiao-Fei , Hao Yu-Jin 
Published: June 2017

Abstract
The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.
Contrasting mechanisms of growth in two model rod-shaped bacteria
Authors: Billaudeau Cyrille , Chastanet Arnaud , Yao Zhizhong , Cornilleau Charlène , Mirouze Nicolas , Fromion Vincent , Carballido-López Rut 
Published: June 2017

AbstractHow cells control their shape and size is a long-standing question in cell biology. Many rod-shaped bacteria elongate their sidewalls by the action of cell wall synthesizing machineries that are associated to actin-like MreB cortical patches. However, little is known about how elongation is regulated to enable varied growth rates and sizes. Here we use total internal reflection fluorescence microscopy and single-particle tracking to visualize MreB isoforms, as a proxy for cell wall synthesis, in Bacillus subtilis and Escherichia coli cells growing in different media and during nutrient upshift. We find that these two model organisms appear to use orthogonal strategies to adapt to growth regime variations: B. subtilis regulates MreB patch speed, while E. coli may mainly regulate the production capacity of MreB-associated cell wall machineries. We present numerical models that link MreB-mediated sidewall synthesis and cell elongation, and argue that the distinct regulatory mechanism employed might reflect the different cell wall integrity constraints in Gram-positive and Gram-negative bacteria.
River plastic emissions to the world’s oceans
Authors: Lebreton Laurent C. M. , van der Zwet Joost , Damsteeg Jan-Willem , Slat Boyan , Andrady Anthony , Reisser Julia 
Published: June 2017

AbstractPlastics in the marine environment have become a major concern because of their persistence at sea, and adverse consequences to marine life and potentially human health. Implementing mitigation strategies requires an understanding and quantification of marine plastic sources, taking spatial and temporal variability into account. Here we present a global model of plastic inputs from rivers into oceans based on waste management, population density and hydrological information. Our model is calibrated against measurements available in the literature. We estimate that between 1.15 and 2.41 million tonnes of plastic waste currently enters the ocean every year from rivers, with over 74% of emissions occurring between May and October. The top 20 polluting rivers, mostly located in Asia, account for 67% of the global total. The findings of this study provide baseline data for ocean plastic mass balance exercises, and assist in prioritizing future plastic debris monitoring and mitigation strategies.
Dendro-dendritic cholinergic excitation controls dendritic spike initiation in retinal ganglion cells
Authors: Brombas A. , Kalita-de Croft S. , Cooper-Williams E. J. , Williams S. R. 
Published: June 2017

AbstractThe retina processes visual images to compute features such as the direction of image motion. Starburst amacrine cells (SACs), axonless feed-forward interneurons, are essential components of the retinal direction-selective circuitry. Recent work has highlighted that SAC-mediated dendro-dendritic inhibition controls the action potential output of direction-selective ganglion cells (DSGCs) by vetoing dendritic spike initiation. However, SACs co-release GABA and the excitatory neurotransmitter acetylcholine at dendritic sites. Here we use direct dendritic recordings to show that preferred direction light stimuli evoke SAC-mediated acetylcholine release, which powerfully controls the stimulus sensitivity, receptive field size and action potential output of ON-DSGCs by acting as an excitatory drive for the initiation of dendritic spikes. Consistent with this, paired recordings reveal that the activation of single ON-SACs drove dendritic spike generation, because of predominate cholinergic excitation received on the preferred side of ON-DSGCs. Thus, dendro-dendritic release of neurotransmitters from SACs bi-directionally gate dendritic spike initiation to control the directionally selective action potential output of retinal ganglion cells.
WASH maintains NKp46+ ILC3 cells by promoting AHR expression
Authors: Xia Pengyan , Liu Jing , Wang Shuo , Ye Buqing , Du Ying , Xiong Zhen , Han Ze-Guang , Tong Liang , Fan Zusen 
Published: June 2017

AbstractInnate lymphoid cells (ILCs) communicate with other haematopoietic and non-haematopoietic cells to regulate immunity, inflammation and tissue homeostasis. How these ILC lineages develop and are maintained is not clear. Here we show that WASH is highly expressed in the nucleus of group 3 ILCs (ILC3s). WASH deletion impairs the cell pool of NKp46+ ILC3s. In NKp46+ ILC3s, WASH recruits Arid1a to the Ahr promoter thus activating AHR expression. WASH deletion in ILC3s decreases the number of NKp46+ ILC3s. Moreover, Arid1a deletion impedes AHR expression and impairs the maintenance of NKp46+ ILC3s. Therefore, WASH-mediated AHR expression has a critical function in the maintenance of NKp46+ ILC3s.
The CaMKII holoenzyme structure in activation-competent conformations
Authors: Myers Janette B. , Zaegel Vincent , Coultrap Steven J. , Miller Adam P. , Bayer K. Ulrich , Reichow Steve L. 
Published: June 2017

AbstractThe Ca2+/calmodulin-dependent protein kinase II (CaMKII) assembles into large 12-meric holoenzymes, which is thought to enable regulatory processes required for synaptic plasticity underlying learning, memory and cognition. Here we used single particle electron microscopy (EM) to determine a pseudoatomic model of the CaMKIIα holoenzyme in an extended and activation-competent conformation. The holoenzyme is organized by a rigid central hub complex, while positioning of the kinase domains is highly flexible, revealing dynamic holoenzymes ranging from 15–35 nm in diameter. While most kinase domains are ordered independently, ∼20% appear to form dimers and <3% are consistent with a compact conformation. An additional level of plasticity is revealed by a small fraction of bona-fide 14-mers (<4%) that may enable subunit exchange. Biochemical and cellular FRET studies confirm that the extended state of CaMKIIα resolved by EM is the predominant form of the holoenzyme, even under molecular crowding conditions.
Type-2 innate lymphoid cells control the development of atherosclerosis in mice
Authors: Newland Stephen A. , Mohanta Sarajo , Clément Marc , Taleb Soraya , Walker Jennifer A. , Nus Meritxell , Sage Andrew P. , Yin Changjun , Hu Desheng , Kitt Lauren L. , Finigan Alison J. , Rodewald Hans-Reimer , Binder Christoph J. , McKenzie Andrew N. J. , Habenicht Andreas J. , Mallat Ziad 
Published: June 2017

AbstractType-2 innate lymphoid cells (ILC2) are a prominent source of type II cytokines and are found constitutively at mucosal surfaces and in visceral adipose tissue. Despite their role in limiting obesity, how ILC2s respond to high fat feeding is poorly understood, and their direct influence on the development of atherosclerosis has not been explored. Here, we show that ILC2 are present in para-aortic adipose tissue and lymph nodes and display an inflammatory-like phenotype atypical of adipose resident ILC2. High fat feeding alters both the number of ILC2 and their type II cytokine production. Selective genetic ablation of ILC2 in Ldlr−/− mice accelerates the development of atherosclerosis, which is prevented by reconstitution with wild type but not Il5−/− or Il13−/− ILC2. We conclude that ILC2 represent a major innate cell source of IL-5 and IL-13 required for mounting atheroprotective immunity, which can be altered by high fat diet.
Chloroplast Genomic Resource of Paris for Species Discrimination
Authors: Song Yun , Wang Shaojun , Ding Yuanming , Xu Jin , Li Ming Fu , Zhu Shuifang , Chen Naizhong 
Published: June 2017

AbstractParis is famous in China for its medicinal value and has been included in the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, and domestication. Due to the plasticity of the morphological characteristics, correct identification among Paris species remains problematic. In this regard, we report the complete chloroplast genome of P. thibetica and P. rugosa to develop highly variable molecular markers. Comparing three chloroplast genomes, we sought out the most variable regions to develop the best cpDNA barcodes for Paris. The size of Paris chloroplast genome ranged from 162,708 to 163,200 bp. A total of 134 genes comprising 81 protein coding genes, 45 tRNA genes and 8 rRNA genes were observed in all three chloroplast genomes. Eight rapidly evolving regions were detected, as well as the difference of simple sequence repeats (SSR) and repeat sequence. Two regions of the coding gene ycf1, ycf1a and ycf1b, evolved the quickest and were proposed as core barcodes for Paris. The complete chloroplast genome sequences provide more integrated and adequate information for better understanding the phylogenetic pattern and improving efficient discrimination during species identification.
FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts
Authors: Smith Edward R. , Tan Sven-Jean , Holt Stephen G. , Hewitson Tim D. 
Published: June 2017

AbstractIn kidney disease, higher circulating levels of the mineral-regulating hormone fibroblast growth factor (FGF)-23 are predictive of disease progression but direct pathogenic effects on the kidney are unknown. We sought evidence of local renal synthesis in response to unilateral ureteric obstruction in the mouse, and pro-fibrotic actions of FGF23 on the fibroblast in vitro. Acute tubulointerstitial injury due to unilateral ureteric obstruction stimulated renal FGF23 synthesis by tubules, and downregulated inactivating proprotein convertases, without effects on systemic mineral metabolism. In vitro, FGF23 had divergent effects on fibroblast activation in cells derived from normal and obstructed kidneys. While FGF23 failed to stimulate fibrogenesis in normal fibroblasts, in those primed by injury, FGF23 induced pro-fibrotic signalling cascades via activation of TGF-β pathways. Effects were independent of α-klotho. Tubule-derived FGF23 may amplify myofibroblast activation in acute renal injury, and might provide a novel therapeutic target in renal fibrosis.
The importance of naming cryptic species and the conservation of endemic subterranean amphipods
Authors: Delić Teo , Trontelj Peter , Rendoš Michal , Fišer Cene 
Published: June 2017

AbstractMolecular taxonomy often uncovers cryptic species, reminding us that taxonomic incompleteness is even more severe than previous thought. The importance of cryptic species for conservation is poorly understood. Although some cryptic species may be seriously threatened or otherwise important, they are rarely included in conservation programs as most of them remain undescribed. We analysed the importance of cryptic species in conservation by scrutinizing the South European cryptic complex of the subterranean amphipod Niphargus stygius sensu lato. Using uni- and multilocus delineation methods we show that it consists of 15 parapatric and sympatric species, which we describe using molecular diagnoses. The new species are not mere “taxonomic inflation” as they originate from several distinct branches within the genus and coexist with no evidence of lineage sharing. They are as evolutionarily distinct as average nominal species of the same genus. Ignoring these cryptic species will underestimate the number of subterranean endemics in Slovenia by 12 and in Croatia by four species, although alpha diversity of single caves remains unchanged. The new taxonomy renders national Red Lists largely obsolete, as they list mostly large-ranged species but omit critically endangered single-site endemics. Formal naming of cryptic species is critical for them to be included in conservation policies and faunal listings.
Politeness and Compassion Differentially Predict Adherence to Fairness Norms and Interventions to Norm Violations in Economic Games
Authors: Zhao Kun , Ferguson Eamonn , Smillie Luke D. 
Published: June 2017

AbstractAdherence to norms and interventions to norm violations are two important forms of social behaviour modelled in economic games. While both appear to serve a prosocial function, they may represent separate mechanisms corresponding with distinct emotional and psychological antecedents, and thus may be predicted by different personality traits. In this study, we compared adherence to fairness norms in the dictator game with responses to violations of the same norms in third-party punishment and recompensation games with respect to prosocial traits from the Big Five and HEXACO models of personality. The results revealed a pattern of differential relations between prosocial traits and game behaviours. While norm adherence in the dictator game was driven by traits reflecting good manners and non-aggression (the politeness aspect of Big Five agreeableness and HEXACO honesty-humility), third-party recompensation of victims—and to a lesser extent, punishment of offenders—was uniquely driven by traits reflecting emotional concern for others (the compassion aspect of Big Five agreeableness). These findings demonstrate the discriminant validity between similar prosocial constructs and highlight the different prosocial motivations underlying economic game behaviours.