bluuebunny/crossref_metadata_2025_split huggingface dataset:
EOMES‐positive CD4+ T cells are increased in PTPN22 (1858T) risk allele carriers
Authors: Chemin Karine , Ramsköld Daniel , Diaz‐Gallo Lina‐Marcela , Herrath Jessica , Houtman Miranda , Tandre Karolina , Rönnblom Lars , Catrina Anca , Malmström Vivianne 
Published: February 2018

AbstractThe presence of the PTPN22 risk allele (1858T) is associated with several autoimmune diseases including rheumatoid arthritis (RA). Despite a number of studies exploring the function of PTPN22 in T cells, the exact impact of the PTPN22 risk allele on T‐cell function in humans is still unclear. In this study, using RNA sequencing, we show that, upon TCR‐activation, naïve human CD4+ T cells homozygous for the PTPN22 risk allele overexpress a set of genes including CFLAR and 4‐1BB, which are important for cytotoxic T‐cell differentiation. Moreover, the protein expression of the T‐box transcription factor Eomesodermin (EOMES) was increased in T cells from healthy donors homozygous for the PTPN22 risk allele and correlated with a decreased number of naïve CD4+ T cells. There was no difference in the frequency of other CD4+ T‐cell subsets (Th1, Th17, Tfh, Treg). Finally, an accumulation of EOMES+CD4+ T cells was observed in synovial fluid of RA patients with a more pronounced production of Perforin‐1 in PTPN22 risk allele carriers. Altogether, we propose a novel mechanism of action of PTPN22 risk allele through the generation of cytotoxic CD4+ T cells and identify EOMES+CD4+ T cells as a relevant T‐cell subset in RA pathogenesis.
Effect of local wound infiltration with ketamine versus dexmedetomidine on postoperative pain and stress after abdominal hysterectomy, a randomized trial
Authors: Mohamed S.A. , Sayed D.M. , El Sherif F.A. , Abd El‐Rahman A.M. 
Published: February 2018

AbstractBackground and ObjectivesPostoperative pain and stress elicit hormonal changes. We aimed at comparing the effects of wound infiltration with ketamine versus dexmedetomidine on postoperative pain and stress response.MethodsThis double‐blinded study included ninety patients scheduled for total abdominal hysterectomy and were randomly assigned into three groups to receive local wound infiltration with 40 mL of 0.25% bupivacaine (group C), plus 2 mg/kg ketamine (group K) or 2 μg/kg dexmedetomidine (group D). Primary outcome was postoperative morphine consumption; secondary outcomes included first request of analgesia, VAS scores at rest and movement (VAS–R/M) and side effects. Serum cortisol, prolactin and glucose levels at baseline, pre‐infiltration, 6 and 24 h postoperatively were measured.ResultsRescue analgesia was less in K (6.80 ± 3.19 mg) and D (8.39 ± 3.86 mg) compared to C (13.33 ± 4.01 mg) (p < 0.05). First request of analgesia was delayed in K (7.60 ± 4.16 h) and D (6.00 ± 3.73 h) compared to C (4.20 ± 1.13 h) (p < 0.05). Both VAS and R/M were significantly lower in K (all over 24 h) and D (for 8 and 4 h, respectively) compared to C. Stress markers were significantly lower in K and D compared to C at 6 and 24 h, and in K compared to D at 24 h (p < 0.05).ConclusionsLocal wound infiltration with ketamine or dexmedetomidine added to bupivacaine had an opioid‐sparing effect, delayed first request of rescue analgesia, and attenuated postoperative stress response, especially with ketamine in patients underwent total abdominal hysterectomy.
Facile Synthesis of Cyclodextrin Functionalized Reduced Graphite Oxide with the Aid of Ionic Liquid for Simultaneous Determination of Guanine and Adenine
Authors: Wang Meiling , Cui Mingzhu , Liu Weifeng , Liu Xuguang , Xu Bingshe 
Published: February 2018

AbstractHere, a facile reduction approach for synthesis of β‐cyclodextrin functionalized reduced graphite oxide with the aid of ionic liquid (βCD/IRGO) was reported. This strategy relies on the simple mixing of pre‐pared aqueous βCD/GO with ionic liquid (IL) at high temperatures, which can bring about a well dispersed βCD/RGO in the utilized IL (βCD/IRGO) with a synchronous evaporation of water. The as‐prepared βCD/IRGO product can be stably re‐dispersed in water (up to 1.0 mg/mL) after washed and dried. And the presented βCD/IRGO composites possess high supramolecular recognition capability and good conductivity for realizing enhanced electrochemical response towards simultaneous detection of guanine and adenine compared with other βCD/carbon (RGO or carbon nanotube) based composites. In the presence of one of analysts, the linear response ranges is 0.03–10.0 μM for guanine and 0.02–7.0 μM for adenine, respectively, with detection limit of 0.01 μM for both guanine and adenine (S/N=3). This ease of making the βCD/IRGO composite will open up possibilities of its diversifier applications.
Screen‐printed Gold Electrodes Functionalized with Grafted p‐Aminobenzoic Acid for the Construction of Electrochemical Immunosensors. Determination of TGF‐β1 Cytokine in Human Plasma
Authors: Guerrero Sara , Agüí Lourdes , Yáñez‐Sedeño Paloma , Pingarrón José M. 
Published: February 2018

AbstractScreen‐printed gold electrodes were modified with grafted p‐aminobezoic acid to provide scaffolds with high loading of surface confined carboxyl groups that can be used to construct electrochemical immunosensors. As a specific application, an immunosensor for the determination of the cytokine transforming growth factor beta 1 (TGF‐β1) is reported. The capture antibody (anti‐TGF) was covalently immobilized on the surface of the prepared disposable scaffold and a sandwich‐type immunoassay was implemented with biotinylated detector antibody (Biotin‐anti‐TGF) and streptavidin labeled with alkaline phosphatase (AP‐Strept). The transduction event was monitored by differential pulse voltammetry after addition of 1‐naphthyl phosphate (1‐NPP) as the enzyme substrate. The different variables affecting the preparation and the analytical behavior of the AP‐Strept‐Biotin‐anti‐TGF‐TGF‐β1‐anti‐TGF‐SPAuE immunosensor were optimized, and the functionalized gold electrodes and the prepared immunosensor were characterized using several techniques. A linear range extending between 0.05 and 3.0 ng/mL TGF‐β1, adequate for the determination of the cytokine in human plasma, and a LOD of 10 pg/mL, better than that claimed for commercial ELISA kits, were achieved. The immunosensor exhibits also a great selectivity against other proteins and an excellent storage ability. The usefulness of the immunosensor was demonstrated by analyzing human plasma spiked with TGF‐β1.
Toxicokinetics of cadmium in Palaemon varians postlarvae under waterborne and/or dietary exposure
Authors: Pavlaki Maria D. , Morgado Rui G. , Soares Amadeu M.V.M. , Calado Ricardo , Loureiro Susana 
Published: February 2018

Abstract

The present study assessed cadmium uptake and depuration rates in the euryhaline estuarine shrimp Palaemon varians under different exposure routes. Postlarval shrimp were exposed for 4 d under different exposure routes: contaminated water, contaminated diet, and a 2-way exposure scenario where both contaminated water and diet were used. After exposure, postlarval shrimp were transferred to a clean medium and fed a noncontaminated diet for 96 h. Bioaccumulation via the different exposure routes was modeled with a standard first-order, one-compartment toxicokinetics model and one with an additional parameter reflecting an inert fraction or storage compartment. The simultaneous 2-way exposure (through water and diet) resulted in accumulation being almost twice as high as the sum of the individual exposure routes, thus indicating that accumulation from multiple routes may be more than additive. Cadmium uptake from water was faster than uptake from food maintained for 48 h at that same cadmium concentration. Shrimp were unable to eliminate cadmium from their body, showing no depuration during 96 h after exposure via different routes, thus suggesting that a longer depuration period is needed. Model comparisons did not provide a significantly better fit when the model included the presence of an inert fraction. The present study highlights the importance of assessing accumulation using multiple exposure routes compared with individual routes because the latter may underestimate bioaccumulation. Environ Toxicol Chem 2018;37:1614–1622. © 2018 SETAC
Characterization of rammed‐earth materials from the XVIth century Badii Palace in Marrakech, Morocco to ensure authentic and reliable restoration
Authors: Daoudi L. , Rocha F. , Costa C. , Arrebei N. , Fagel N. 
Published: February 2018

AbstractThirty rammed‐earth and coating materials collected from the Badii Palace wall in Marrakech were investigated for their particle size, chemical, and mineralogical characteristics. This was done in order to assess the original methods employed—and the provenance of the earth products used—during the Saadian period (XVIth century) construction of the palace. Differences in composition between the rammed‐earth and coating materials (and comparison with the local raw materials) suggest a different origin for these two materials. A high compositional similarity between the coating material and the corresponding rammed‐ earth is observed for the samples collected from well‐preserved walls. However, incompatibility between rammed earth and coatings was observed in many parts of the wall where the original coated materials have deteriorated by natural weathering and by the corrosive action of atmospheric pollution. The composition of coating material currently used for restoration shows the same incompatibility with the substrate and this is probably related to the same degradation problems. Finally, the influence of the semiarid climate in the degradation process is discussed. This study indicates constraints on the selection of the most suitable materials for sustainable restoration of these historic buildings.
Multiscale energy reallocation during low‐frequency steady‐state brain response
Authors: Wang Yifeng , Chen Wang , Ye Liangkai , Biswal Bharat B. , Yang Xuezhi , Zou Qijun , Yang Pu , Yang Qi , Wang Xinqi , Cui Qian , Duan Xujun , Liao Wei , Chen Huafu 
Published: February 2018

AbstractTraditional task‐evoked brain activations are based on detection and estimation of signal change from the mean signal. By contrast, the low‐frequency steady‐state brain response (lfSSBR) reflects frequency‐tagging activity at the fundamental frequency of the task presentation and its harmonics. Compared to the activity at these resonant frequencies, brain responses at nonresonant frequencies are largely unknown. Additionally, because the lfSSBR is defined by power change, we hypothesize using Parseval's theorem that the power change reflects brain signal variability rather than the change of mean signal. Using a face recognition task, we observed power increase at the fundamental frequency (0.05 Hz) and two harmonics (0.1 and 0.15 Hz) and power decrease within the infra‐slow frequency band (<0.1 Hz), suggesting a multifrequency energy reallocation. The consistency of power and variability was demonstrated by the high correlation (r > .955) of their spatial distribution and brain–behavior relationship at all frequency bands. Additionally, the reallocation of finite energy was observed across various brain regions and frequency bands, forming a particular spatiotemporal pattern. Overall, results from this study strongly suggest that frequency‐specific power and variability may measure the same underlying brain activity and that these results may shed light on different mechanisms between lfSSBR and brain activation, and spatiotemporal characteristics of energy reallocation induced by cognitive tasks.
Assessment of Land Subsidence and Climate Change Impacts on Inundation Hazard in Southwestern Taiwan
Authors: Wang Hsiao‐Wen , Lin Cheng‐Wei , Yang Chun‐Yao , Ding Chung‐Feng , Hwung Hwung‐Hweng , Hsiao Shih‐Chun 
Published: February 2018

AbstractExcessive extraction of groundwater resulting in serious land subsidence as well as intensified rainfall and storm surges due to climate change complicate the flooding problems in southwest Taiwan. A coupled set of different models was proposed to analyze the effect of inundation risk considering land subsidence and climate change. Three models, including the groundwater flow model, land subsidence model, and the physiographic drainage–inundation model, were used in this study, enabling simulations of different considerations. The results revealed that more severe flooding would result from land subsidence and climate change. The findings of a 21% increase in flood area with an inundation depth greater than 1.5 m for 200‐yr return period events clearly showed more severe flooding would result from land subsidence. The flooding in those severely subsiding areas would increase in a range from 3.4 to 21.5% when further considering climate change.While the simulation results revealed that the flood area could be decreased by as much as 50% taking into account the implemented policies, the coastal region would still be exposed to a high risk of being flooded. It thus suggests that policies focusing on infrastructure would be insufficient, and river basin management as well as spatial planning should be investigated further. Copyright © 2018 John Wiley & Sons, Ltd.
The miR‐203/SNAI2 axis regulates prostate tumor growth, migration, angiogenesis and stemness potentially by modulating GSK‐3β/β‐CATENIN signal pathway
Authors: Tian Xinxin , Tao Fangfang , Zhang Baotong , Dong Jin‐Tang , Zhang Zhiqian 
Published: February 2018

AbstractDysregulation of microRNA expression plays a pivotal role in the initiation and progression of a variety of human carcinomas including prostate cancer. Our previous studies have demonstrated that the silence of miR‐203 contributes to the invasiveness of malignant breast cancer cells by targeting SNAI2. However, the effects and underlying mechanisms of miR‐203/SNAI2 axis in prostate cancer have not been elucidated. The aim of this study is to explore the effects of miR‐203/SNAI2 axis on the biological characteristics of prostate carcinomas both in vitro and in vivo. We found that miR‐203 was significantly downregulated in prostate cancer cell lines compared with immortalized prostate epithelial cells using semi‐quantitative PCR and real‐time PCR, as well as in clinical prostate cancer tissues compared to normal tissues using TCGA analysis. Functionally, miR‐203 inhibited prostate cancer cell proliferation, migration, endothelial cell tube formation and cancer stemness in vitro. Meanwhile, overexpression of miR‐203 suppressed SNAI2 expression both in DU145 and PC3 cells. In addition, the in vivo study showed that miR‐203 suppressed tumorigenicity, metastasis and angiogenesis of DU145 cells. Ectopic expression of SNAI2 rescued the inhibitory effects of miR‐203 both in vitro and in vivo. Importantly, the EMT markers CDH1 and VIMENTIN were modulated by the miR‐203/SNAI2 axis. Furthermore, the GSK‐3β/β‐CATENIN signal pathway was suppressed by miR‐203 and could be reactivated by SNAI2. Taken together, this research unveiled the function of miR‐203/SNAI2 axis in tumorigenesis, angiogenesis, stemness, metastasis and GSK‐3β/β‐CATENIN signal pathway in prostate cancer and gave insights into miR‐203/SNAI2‐targeting therapy for prostate cancer patients. © 2018 IUBMB Life, 70(3):224–236, 2018
A novel anti‐cancer agent, FPDHP, induces anoikis in various human cancer cells through activation of calpain, and downregulation of anoikis‐related molecules
Authors: Kim Seon Goo , Yooun Ji Hea , Kim Dong Eun , Lee Eung‐Seok , Kwon Taeg Kyu , Kim Shin , Park Jong Wook 
Published: February 2018

AbstractResistance to anoikis and growth in anchorage‐independent conditions are hallmarks of highly metastatic cancer cells. Anoikis is a type of apoptosis induced by inadequate cell/extracellular matrix (ECM) attachment and an attractive anti‐cancer therapeutic strategy in cancer chemotherapeutic field. Therefore, the development of anoikis‐inducing agents is useful and promising to overcome cancer. When FPDHP, a novel anoikis‐inducing agent, was treated within 3 h, FPDHP induced massive cell detachment in various human cancer cells, irrespective of apoptosis. Moreover, FPDHP decreased the expression of integrins, FAK, focal adhesion signaling effectors (talin1 and talin2), tight junction proteins (ZO‐1, ZO‐2, and ZO‐3), transcriptional mediators of epithelial‐mesenchymal transition (EMT) (Snail1 and Snail2), and anoikis‐related protein, such as Mcl‐1 (L). Interestingly, Caki/ZO‐2 and Caki/α6 are significantly suppressed the FPDHP‐mediated cell detachment, and the constitutive active form of Akt and overexpression of Mcl‐1 (L) partially inhibited the cellular detachment induced by FPDHP. On the other hand, when FPDHP was treated for more than 12 h, FPDHP induced caspase‐dependent apoptosis and release of AIF and cytochrome c from mitochondria. Furthermore, FPDHP down regulated Mcl‐1 (L) at post‐transcriptional level, and overexpression of Mcl‐1 (L) partially attenuated the apoptosis induced by FPDHP. Additionally, PD150606, a calpain inhibitor, attenuated FPDHP‐mediated cell detachment and apoptosis. Taken together, these results suggest that FPDHP possesses anoikis‐inducing activity or potential making cancer cells susceptible to anoikis, and may be developed as a novel active compound for cancer treatment.
Improvement of bacterial methane elimination using porous ceramsite as biocarrier
Authors: Sun Meng‐Ting , Yang Zhi‐Man , Lu Jun , Fan Xiao‐Lei , Guo Rong‐Bo , Fu Shan‐Fei 
Published: February 2018

AbstractBACKGROUNDMethane is a greenhouse gas (GHG) which contributes to climate change. Biofiltration with immobilized methane‐oxidizing bacteria (MOB) is a promising option to eliminate methane. In order to achieve high methane removal efficiency (RE), the appropriate carrier material with favorable characteristics to perform well in MOB immobilization and methane elimination, must be selected.RESULTSA MOB consortium was enriched from landfill soil and immobilized on porous materials to eliminate methane at high (∼20% (v/v)) and low (∼1% (v/v)) concentrations. The methane elimination capacities of immobilized MOB were evaluated and the microbial immobilization abilities of materials were compared. Results showed that MOB inoculated in black ceramsite (BC) permitted the highest elimination capacity (EC) of 5.36 ± 0.29 µmol h‐1 cm‐3 at the methane concentration of ∼20% (v/v), which was almost 3.6 times that of suspended cells. At a methane concentration of ∼1% (v/v), the MOB incorporated with red ceramsite (RC) exhibited the optimal EC of 1.48 ± 0.03 µmol h‐1 cm‐3, which was 64% higher than the control. Biophosphorus tests showed that BC and RC could immobilize more MOB cells than active carbon (AC), and SEM images, MB adsorption tests, BET and FTIR indicated that their large pore and surface properties might favor MOB immobilization.CONCLUSIONCeramsite with desirable porosity and surface properties could promote MOB immobilization, and further improve the methane elimination capacity, and might be a favorable biocarrier in MOB biofilters. © 2018 Society of Chemical Industry
Innate immunity and cellular senescence: The good and the bad in the developmental and aged brain
Authors: Santoro Antonietta , Spinelli Chiara Carmela , Martucciello Stefania , Nori Stefania Lucia , Capunzo Mario , Puca Annibale Alessandro , Ciaglia Elena 
Published: February 2018

AbstractOngoing studies evidence cellular senescence in undifferentiated and specialized cells from tissues of all ages. Although it is believed that senescence plays a wider role in several stress responses in the mature age, its participation in certain physiological and pathological processes throughout life is coming to light. The “senescence machinery” has been observed in all brain cell populations, including components of innate immunity (e.g., microglia and astrocytes). As the beneficial versus detrimental implications of senescence is an open question, we aimed to analyze the contribution of immune responses in regulatory mechanisms governing its distinct functions in healthy (development, organogenesis, danger patrolling events) and diseased brain (glioma, neuroinflammation, neurodeneration), and the putative connection between cellular and molecular events governing the 2 states. Particularly this review offers new insights into the complex roles of senescence both as a chronological event as age advances, and as a molecular mechanism of brain homeostasis through the important contribution of innate immune responses and their crosstalk with neighboring cells in brain parenchyma. We also highlight the impact of the recently described glymphatic system and brain lymphatic vasculature in the interplay between peripheral and central immune surveillance and its potential implication during aging. This will open new ways to understand brain development, its deterioration during aging, and the occurrence of several oncological and neurodegenerative diseases.
CD71+ erythroid cells from neonates born to women with preterm labor regulate cytokine and cellular responses
Authors: Miller Derek , Romero Roberto , Unkel Ronald , Xu Yi , Vadillo-Ortega Felipe , Hassan Sonia S , Gomez-Lopez Nardhy 
Published: February 2018

Abstract
Neonatal CD71+ erythroid cells are thought to have immunosuppressive functions. Recently, we demonstrated that CD71+ erythroid cells from neonates born to women who underwent spontaneous preterm labor (PTL) are reduced to levels similar to those of term neonates; yet, their functional properties are unknown. Herein, we investigated the functionality of CD71+ erythroid cells from neonates born to women who underwent spontaneous preterm or term labor. CD71+ erythroid cells from neonates born to women who underwent PTL displayed a similar mRNA profile to that of those from term neonates. The direct contact between preterm or term neonatal CD71+ erythroid cells and maternal mononuclear immune cells, but not soluble products from these cells, induced the release of proinflammatory cytokines and a reduction in the release of TGF-β. Moreover, PTL-derived neonatal CD71+ erythroid cells (1) modestly altered CD8+ T cell activation; (2) inhibited conventional CD4+ and CD8+ T-cell expansion; (3) suppressed the expansion of CD8+ regulatory T cells; (4) regulated cytokine responses mounted by myeloid cells in the presence of a microbial product; and (5) indirectly modulated T-cell cytokine responses. In conclusion, neonatal CD71+ erythroid cells regulate neonatal T-cell and myeloid responses and their direct contact with maternal mononuclear cells induces a proinflammatory response. These findings provide insight into the biology of neonatal CD71+ erythroid cells during the physiologic and pathologic processes of labor.
Preparation of fish waste silage and its effect on the growth performance and meat quality of broiler chickens
Authors: Shabani Akram , Boldaji Fatollah , Dastar Behrouz , Ghoorchi Taghi , Zerehdaran Saeed 
Published: February 2018

AbstractBACKGROUNDThis experiment was conducted to evaluate the effects of replacing soybean meal with fish‐waste silage (FWS) in diets on growth performance and meat quality in broiler chickens. Fish waste (FW) was fermented with molasses and Lactobacillus plantarum and Aspergillus oryzae for 15 days. A total of 240 day‐old male Cobb 500 broiler chickens were used in a 42‐day experiment in which the birds were randomly allotted to one of five dietary treatments (containing 0, 30, 60, 90, and 120 g kg−1 FWS) in a completely randomized design.RESULTSResults indicated that the fermentation process significantly reduced pH and ether extract, but it increased crude protein content and lactic acid bacteria count in FW. The use of FWS significantly improved daily weight gain and feed conversion ratio of broilers. The amount of thiobarbiturate‐reducing substances and lipid content in chicken's meat fed diet containing FWS was significantly lower than in the group fed a control diet.CONCLUSIONIn conclusion, the microbial fermentation process is effective in improving nutritive values of FW. Feeding up to 120 g kg−1 FWS also improves the performance and meat quality of birds and can be used as a suitable protein source in broiler chickens' diet. © 2018 Society of Chemical Industry