bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Human Papillomavirus-Associated Cervical Cancer Differentially Expressed Genes and Identification of Prognostic Factors using Integrated Bioinformatics Approaches
Authors: Hatefi-Shogae Saba , Emadi-Baygi Modjtaba , Ghaedi-Heydari Rasoul 
Published: September 2024

Background:
Human papillomavirus (HPV)-induced cervical cancer progresses through a series of steps. Despite our limited understanding of the mechanisms driving this progression, identifying the key genes involved could significantly improve early detection and treatment.

Materials and Methods:
Two gene expression profiles of GSE9750 and GSE6791, which included cervical cancer HPV-positive and -negative samples, were evaluated using the R limma package with established cut-off criteria of P value < 0.05 and | fold change| ≥ 1. KEGG pathway enrichment was performed to identify potential pathways. Weighted gene co-expression network analysis (WGCNA) was used to discover co-expressed gene modules and trait–module connections.

Results:
Considering the defined criteria, 115 differentially expressed genes (DEGs) were identified. The DEG’s KEGG pathway enrichment analysis revealed enrichment in highly relevant pathways to the HPV infection, including cell cycle, viral carcinogenesis, autophagy-animal, Epstein-Barr virus infection, human T-cell leukemia virus 1 infection, and microRNAs in cancer. WGCNA results in 13 co-expression modules, and the magenta module is identified with significant relations to HPV, cervical cancer stage, and metastasis traits. The survival analysis identified BEX1 and CDC45 as potential prognostic factors in HPV-associated cervical cancer.

Conclusion:
The innovation of our work lies in identifying essential genes associated with the multi-step process of cervical carcinogenesis. In fact, the current study has the potential to give a distinct viewpoint on the molecular pathways linked to cervical cancer. Considering the potential importance of the hub genes, we recommend conducting in-depth wet lab research to determine their impact on the biological mechanisms of cervical cancer.
Evaluation of MYC Oncogene Expression in Human Breast Cancer and Its Relationship with ACSL4 and Lipin-1 Expression
Authors: Dinarvand Negar , Azizi Reza , Rastaghi Sedighe , Karimi Farzaneh , Sheikhi Abdolkarim , Pourfarzam Morteza 
Published: September 2024

Background:
Disturbances in lipid metabolism are one of the hallmarks of cancer cells. Fatty acid synthesis and oxidation play a crucial role in the proliferation, growth, and survival of cancer cells. Several enzymes are involved in lipid metabolism. MYC is an oncogene and plays various regulatory roles in lipid metabolism. This study aimed to evaluate MYC expression and its association with the expressions of Lipin-1, ACSL4 (enzymes involved in lipid metabolism), in pairs of breast cancer (BC) and adjacent normal tissues to further understand the MYC influence on metabolic regulation.

Materials and Methods:
Fifty-five pairs of samples of BC and noncancerous adjacent tissues were utilized in the present study to analyze MYC, Lipin-1, and ACSL4 by quantitative real-time polymerase chain reaction. Further, the expression of Lipin-1 and ACSL4 proteins and a number of other clinicopathologically relevant variables were studied employing immunohistochemistry staining.

Results:

MYC expression was substantially higher in BC tissues than in adjacent normal tissues, according to our findings. This upregulation was positively correlated with tumor size and stage. Although MYC expression was not correlated with that of ACSL4 and Lipin-1 expression, this may result from the complex metabolic changes that occur when cells become malignant.

Conclusions:
Although further research is required to assess MYC’s impact on tumor metabolic regulation, the correlations seen here between MYC, the pathological stage, and tumor size may indicate its prognostic significance in BC. Hence, it may be considered as a potential therapeutic target for further studies.
Calprotectin Correlates with Reduced Level of LVEF and Occurrence of Cardiac Arrhythmia in STEMI Patients
Authors: Haybar Habib , Ramezani Babak , Assareh Ahmad Reza , Kardooni Ali , Azizidoost Shirin 
Published: September 2024

Background:
Calprotectin is recognized as a promising prognostic as well as a diagnostic marker of cardiac disorders. In the present study, we aimed to survey the efficiency of serum calprotectin levels in anticipating the severity of coronary artery disease (CAD) along with in-hospital major adverse cardiovascular events (MACE) in patients with ST-segment elevation (STEMI) underlying primary percutaneous coronary intervention (PCI).

Materials and Methods:
A total of 97 patients with STEMI participated and were evaluated for in-hospital MACE for possible correlation with serum calprotectin.

Results:
Increased levels of serum calprotectin showed positive and negative correlation with severity of coronary arteries and left ventricular ejection fraction (LVEF) of STEMI patients, respectively. Regarding in-hospital MACE, only arrhythmia showed a significant relationship in patients with high calprotectin levels.

Conclusion:
High calprotectin levels may be a prognostic marker for occluded artery and LVEF in STEMI patients.
Motorcycle Wheel Spoke Injury to the Ankle and Foot in Children: A Cross-sectional Observational Study
Authors: Boubkraoui Mohamed El Mahdi , Rouaghi Hajar , Cherqaoui Yassine , Bjitro Chafik , Aboumaarouf Mustapha , Cherqaoui Abdelmounim 
Published: September 2024

Abstract

Background:
In developing countries, motorcycles are a widespread transportation method, leading to a notable increase in road traffic injuries, particularly those involving the ankle and foot caused by motorcycle wheel spokes.

Aims and Objectives:
Our study sought to understand the epidemiological features and characteristics of motorcycle-induced wheel spoke injuries to the ankle and foot in children. The objectives were to comprehend injury patterns, evaluate their severity and pinpoint clinical indicators predicting injury nature and extent, aiming to guide prevention strategies.

Materials and Methods:
We conducted a cross-sectional observational study with a retrospective data collection focused on children under the age of 16 years who sustained injuries from motorcycle rear wheel spokes while riding as pillion passengers and were admitted to our department over 11 years, from 1 January 2010 to 31 December 2020, with a minimum follow-up of 2 years. Soft tissue wounds were classified using the Oestern-Tscherne classification.

Results:
Thirty patients were enrolled in this study. Before 2015, cases ranged from none to one annually, increasing to 2–7 cases per year after 2015. The average age was 6.7 ± 2.1 years. Significantly, more patients were older than 6 years (P < 0.001), injured on the right side (P < 0.001) and suffered the injuries in the afternoon (P < 0.001). All patients were pillion and were injured by the motorcycle’s rear wheel. Twenty-five patients (83%) had a Grade 3 injury. There was a tendon rupture in 22 (73%) and a bone fracture in 15 (50%) patients. Loss of soft tissue (P = 0.036) and reduced ankle mobility (P = 0.046) were linked to tendon ruptures. Lengths of wound exceeding 6 cm (P = 0.025) and loss of soft tissue (P = 0.025) were associated with a bone fracture.

Conclusion:
Children’s motorcycle wheel spoke injuries have increased in recent years. Loss of soft tissue and ankle mobility deficit proved to be reliable clinical signs of a tendon rupture. A wound length exceeding 6 cm and a loss of soft tissue were indicative of a related bone fracture.
Virilising Adrenal Adenoma in Children
Authors: Verma Anjali , Kadian Yogender Singh , Kajal Pradeep , Ali Md Mokarram , Lata Kusum , Verma Surender 
Published: September 2024

Abstract
Tumours of the adrenal cortex are most commonly seen in adults but rare in children. The clinical manifestations depend on the age and sex of patients; about two-thirds of the cases virilisation is the predominant presentation, whereas many presents with both virilisation and cushingoid features. Diagnosis is confirmed by hormonal evaluation and radiological investigations. Surgical removal is the mainstay of treatment. Usually, there is a good prognosis in paediatric patients, whereas the high mortality rate reported in older literature may have been due to big tumour size, post-operative complications and inadequate steroid replacement. Here, we are presenting a series of three cases during childhood with virilising features due to adrenocortical tumours. All of them presented predominantly with features suggestive of virilisation. All patients underwent surgery and had a good outcome despite big tumour size in one of the patients. Histology revealed a benign lesion in the form of adrenal adenoma. Most virilisation and cushingoid features disappeared in the follow-up (median – 1 year). Although these tumours are rare, a high index of suspicion should be kept in children with cushingoid features, virilisation or a combination of both of them. Even if the tumour size is big, adequate steroid replacement and supportive management postoperatively have led to good prognosis in our patients.
The inhibitory effect of type V transforming growth factor-β receptor antagonist on the proliferation of keloid fibroblasts by suppressing insulin-like growth factor-binding protein 3-interleukin-6 signaling
Authors: Zhou Boya , Lin Xunxun , Xia Lingling , Gao Zhen , Di Meihua , Wu Xiaoli , Wang Wenbo 
Published: September 2024

Abstract

Background:
Hyperplasia of fibroblasts is critical in keloid pathogenesis. Insulin-like growth factor-binding protein 3 (IGFBP3) is an important factor in the regulation of cell growth and type V transforming growth factor-β receptor (TβR-V) is a specific receptor of IGFBP3. However, the role of IGFBP3 in keloid development has not been reported.

Objectives:
This study aimed to investigate the role of IGFBP3 in keloid pathogenesis and evaluate the effects of TβR-V antagonist on keloid fibroblasts (KFs) activities.

Methods:

IGFBP3 expression in keloids and its impact on KF proliferation were examined. The effects of TβR-V antagonist on KF cell proliferation, migration, and invasion were also investigated. Differentially expressed genes (DEGs) between TβR-V antagonist treated and nontreated KFs were identified through RNA-sequencing (RNA-seq), followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses.

Results:

IGFBP3 was overexpressed in keloids and could promote KF proliferation. TβR-V antagonist suppressed KFs proliferation, migration, and invasion. GO and KEGG analyses showed that the downregulated DEGs revealed by RNA-seq were significantly enriched in terms related to cell proliferation. Interleukin-6 (IL-6) was identified as the only gene interacting with IGFBP3 in the PPI network and was associated with nine hub genes. In vitro assay confirmed the suppression of IL-6 by TβR-V antagonist in KFs.

Conclusion:
Our study demonstrated that TβR-V antagonist could inhibit keloid growth likely through suppressing IGFBP3-IL-6 signaling activation. These findings suggest that targeting TβR-V could be a potential therapeutic strategy for keloid treatment.
Treatment outcomes of root perforations repaired by calcium silicate-based cements with or without an accelerator: A randomized controlled trial
Authors: Tungputsa Kanyarat , Banomyong Danuchit , Osiri Sittichoke , Sutimuntanakul Supachai 
Published: September 2024

ABSTRACT

Aim:
To compare the clinical outcomes of root perforations repaired using two calcium silicate-based cements (CSC) with or without accelerator in a randomized controlled trial.

Methods:
The study was designed as a noninferiority, double-blind, randomized controlled trial. Forty-six teeth with root perforations were recruited following the inclusion and exclusion criteria. Teeth were randomly allocated and treated according to the root-repair material into CSC with accelerator (Bio-MA) and CSC without accelerator (ProRoot® MTA) groups. Treatment outcomes of the perforation site and periradicular area were evaluated from clinical and radiographic examination as healed, healing, or disease, where healed and healing were combined and defined as success. Chi-square, Fisher’s exact, and noninferiority analyses were used to analyze the outcomes.

Results:
The recall rate was 100% with a median period of 23.5 months. At the perforation site, healed and healing were 90.5% (19/21) and 9.5% (2/21) in the Bio-MA group and 95.7% (22/23) and 4.3% (1/23) in the ProRoot® MTA group; no disease was found in either group. At the periradicular area, the Bio-MA group had 85.7% (18/21) healed and 14.3% (3/21) healing while ProRoot® MTA group had 82.6% (19/23) healed, 13.0% (3/23) healing, and 4.4% (1/23) disease. No significant difference in treatment outcome, either at the perforation site or periradicular area, was found between the groups (P > 0.05). Bio-MA tended to be noninferior in root-perforation repair compared with ProRoot® MTA.

Conclusion:
Treatment outcomes of root perforation repair using CSC with or without accelerator demonstrated similarly high success rates (P > 0.05).
Assessing the impact of the effect of ozone, casein phosphopeptide–amorphous calcium phosphate, silver diamine fluoride, and hydroxyapatite on remineralization of early carious lesions: An investigation using polarized light microscopic study
Authors: Dhinsa Kavita , Saha Sonali , Ghosh Rangan , Srivastava Shitij , Shekhar Abhinav , Doddawad Vidya Gowdappa 
Published: September 2024

Abstract

Introduction:
A popular trend in dental offices, minimal intervention dentistry emphasizes using the least invasive treatments possible to minimize tissue loss and patient discomfort. The earliest indications of dental caries are white spot lesions that can remineralize. Thus, the effects of four different remineralizing agents on early carious lesions were assessed using polarized light microscopy.

Materials and Methods:
Teeth specimens were immersed in demineralizing agents to create subsurface demineralization. Subsequently, they were randomly assigned to various remineralization regimens, including ozonated water, casein phosphopeptide–amorphous calcium phosphate (CPP-ACP), silver diamine fluoride (SDF), and hydroxyapatite. Each specimen underwent treatment twice daily for 3 min and was then placed in artificial saliva. Mineral content was evaluated at baseline, after demineralization, and during remineralization (on the 7th and 14th day) using a polarized microscopic examination.

Results:
Group 1 demonstrated the most effective remineralization, consistently displaying the lowest lesion depths at both the 7th and 14th days. Post hoc comparisons indicate significant differences between group 1 and other groups, emphasizing the potential superiority of the remineralization process in group 1 compared to the other treatment groups.

Conclusion:
Tooth samples treated with ozonated water, followed by CPP-ACP, SDF, and hydroxyapatite demonstrated a more profound level of remineralization. These agents, namely ozonated water, CPP-ACP, SDF, and hydroxyapatite, prove to be effective in reversing the early stages of enamel caries.

The following core competencies are addressed in this article: Practice-based learning and improvement, Patient care and procedural skills, Systems-based practice, Medical knowledge.
Evaluation and Surveillance of Lateral Elbow Tendinopathy in Manual Workers: Identifying Elbows at Risk
Authors: Hung Chih-Chien , Chen Hsin-Shui , Chang Chih-Hao , Wang Ming-Wei , Hung Kun-Lin , Wang Hsing-Kuo 
Published: September 2024

Abstract

Background:
Manual workers are at risk of developing lateral elbow tendinopathy (LET). We aimed to validate the hypothesis that asymptomatic elbows of manual workers with LET are more likely to have positive physical examination results and morpho-mechanical changes on elbow ultrasonography compared to healthy individuals.

Methods:
This cross-sectional case–control study included 30 manual workers with unilateral LET (LET group) and 30 age- and sex-matched healthy volunteers (control group). Demographic data collection and functional evaluation were performed using the Disabilities of the Arm, Shoulder, and Hand; Patient-Rated Tennis Elbow Evaluation; and European Quality of Life Five-Dimensions questionnaires. Physical examination of the elbows and evaluation of the common extensor tendon (CET) with B-mode morphology ultrasonography and Young’s modulus elastography were performed. The LET-unaffected and LET-affected elbows in the LET group were compared with the elbows in the control group.

Results:
The LET-unaffected elbows in the LET group had a higher prevalence of positive chair test results and focal hypoechoic change and microcalcification on ultrasonography than those in the control group (P < 0.05, 0.005, and 0.05, respectively). In addition, LET-affected elbows showed higher focal hypoechoic change, microcalcification, erosive cortex, and neovascularity than elbows in the control group (P < 0.001, 0.001, 0.005, and 0.001, respectively). The unaffected and affected elbows showed greater CET thickness than those in the control group (P < 0.05 and 0.005).

Conclusion:
The unaffected elbows of manual workers with unilateral LET should be considered at risk of LET. We proposed a risk assessment and surveillance protocol based on chair test results and abnormal ultrasonographic findings.
Abdominal Parasitic Fibroids after Laparoscopic Surgery Using a Power Morcellation: A Novel Insight from Vietnam and a Narrative Revew of the Literature
Authors: Pham Thanh Hai , Nguyen Van Hung , Nguyen Phuc Nhon 
Published: September 2024

Abstract
Parasitic fibroid is a rare benign pathology that can be negotiated because of its rarity. The etiology remains unknown following prior intervention related to uterine fibroids. The assessment of this rare disease is usually based on clinical features and imaging modalities. In addition, appropriate management should be individualized where appropriate. Herein, we report an uncommon case at our center and raise proper recognition for all gynecologists. Particularly, in low-and middle-income countries such as Vietnam, where the health-care system is insufficient to follow-up the long-term postoperation. A 40-year-old woman (gravida 3, para 2) presented to the gynecologic examination room due the abdominal pain and increased size of abdominal circumference. The patient had undergone laparoscopy involving the power morcellator without a contained-bag system for myomectomy at our hospital 6 years prior. The imaging diagnostic tools showed multiple hypoechoic disseminated masses in the abdominal cavity. The laparotomy intervention and histopathological endpoints confirmed completely the diagnosis of parasitic fibroid. Although an extremely rare occurrence, parasitic fibroids should be informed to all patients after laparoscopic intervention for uterine fibroids. In addition, the diagnosis of parasitic fibroid should be warranted among the patients presenting with abdominal pain after a history of fibroid resection. The power morcellation in the contained bag, uterine extraction in hysterectomy, as well as relieving the surgical specimen of uterine fibroids should be gently performed to minimize fibroid tissue disruption.
Inflammatory Myocardial Disease Case Report Following COVID-19 Infection: Giant Cell Myocarditis versus Cardiac Sarcoidosis: To Be or Not to Be
Authors: Jahromi Zeinab Alsadat Fattah , Behnemoon Mahsa , Kamali Monireh , Naderi Nasim 
Published: September 2024

Abstract
COVID-19 can induce cardiac involvement, which can include myocardial infarction, various types of arrhythmia, atrioventricular block, myocarditis, acute heart failure, and sudden cardiac death, in approximately 30% of infected patients. There are some reports on the triggering of sarcoid-like reactions and inflammatory myocardial processes by various types of infections. However, little is known about the relationship between COVID-19 and inflammatory cardiomyopathies such as cardiac sarcoidosis (CS) and giant cell myocarditis (GCM). Herein, we describe the clinical course, management, and follow-up of an unmasked inflammatory cardiomyopathy in a 57-year-old Iranian woman, who presented with ventricular arrhythmia 4 months after mild COVID-19 infection. The onset of symptomatic inflammatory myocardial disorders such as CS or GCM may be triggered by COVID-19 infection. The best approach to the management of myocardial inflammatory COVID-19 should probably be individualized based on the patient’s clinical presentation and severity of cardiac involvement.
Clinical and demographic profile of children with cerebral palsy in Tanta University Hospital
Authors: Eltoukhy Abeer A. , Ismael Sally M. , Darwish Amira Hamed 
Published: September 2024

Background
Cerebral palsy (CP) is a motor disorder characterized by delayed infants and children’s motor development, and abnormal muscle tone, movement, posture, gait, and/or coordination.

Aim
This study aimed to describe clinical types, risk factors, comorbidities, and brain imaging findings in children with CP attending Tanta University Children Hospital.

Patients and methods
The present study included 200 patients with CP aged 1–18 years who attended the pediatric neurology unit at Tanta University Hospital.

Results
Spastic CP was the most common type of CP (66.5%). Perinatal hypoxia was the most common risk factor for CP (30.5%). Intellectual disability and epilepsy were the most common comorbidities reported in 85% and 60%, respectively.

Conclusion
Spastic CP due to perinatal hypoxia is still the most common type of CP. Intellectual disability and epilepsy were the most common comorbidities in children with CP.
The development of metachronous contralateral inguinal hernia after unilateral inguinal hernia repair in infants and children
Authors: Yahia Ahmed M. , Arafa Mohamed A. , Khirallah Mohammad G. , Turki Ali M. 
Published: September 2024

Background
Pediatric Inguinal hernia is the most common surgical procedure in infants and children with a rate of 1–5% in full-term infants. The male-to-female ratio is 5: 1. In Some children with unilateral inguinal hernia, a contralateral one may appear later known as a Metachronous Contralateral Inguinal Hernia (MCIH).

Aims
To determine the incidence and risk factors of MCIH development in infants and children and the necessity of routine contralateral side exploration.

Patients and methods
This was a prospective study that was carried out on 217 infants and children presented with a unilateral inguinal hernia. All children were subjected to ultrasonography of the inguino-scrotal region at the first presentation and a second ultrasonography was done with the appearance of a metachronous hernia.

Results
Increase the risk of developing a MCIH in patients with unilateral inguinal hernia with an age below 6 months, low birth weight (≤2000 gm), immaturity, patients with contralateral internal ring diameter at the primary presentation above or equal 4 mm, and the presence of an umbilical hernia. The side of the ipsilateral hernia and the sex of the patients have less effect on the development of a MCIH. Therefore, a score was built depending on these risk Factors.

Conclusions
The incidence of MCIH development after unilateral hernia repair in this study was 12%. A debate is still present regarding exploring the contralateral side after unilateral inguinal hernia repair.