bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGENDALIAN KROMIUM (CR) YANG TERDAPAT DI LIMBAH BATIK DENGAN METODE FITOREMEDIASI
Authors: Setiyono Andik , Gustaman Rian Arie 
Published: September 2017

Motif dan warna batik dihasilkan dengan pewarna yang mengandung logam berat kromium (Cr). Industri batik secara umum tergolong usaha kecil dan menengah sehingga sebagian besar tidak mengolah limbah batik yang mengandung logam berat Cr. Tujuan penelitian ini adalah memberikan alternatif solusi dari dampak pencemaran Cr dengan memanfaatkan berbagai tumbuhan untuk menyerap Cr pada limbah batik. Penelitian ini adalah kuasi eksperimen dengan rancangan desain pre and post test. Tanaman air yang dipilih adalah enceng gondok (Eichornia crassipes), kayu apu (Pistia stratiotes), dan kayambang (Salvinia cucullata) yang ditanam pada wadah yang berisi air limbah selama 5 hari. Konsentrasi Cr pada air limbah kelompok 1 menurun dari 0,0546 mg/l menjadi 0,0378 mg/l setelah diberi perlakuan dengan enceng gondok. Konsentrasi Cr pada air limbah kelompok 2 menurun dari 0,0488 mg/l menjadi 0,0315 mg/l setelah diberi perlakuan dengan kayambang. Konsentrasi Cr pada air limbah kelompok 1 menurun dari 0,0464 mg/l menjadi 0,0240 mg/l setelah diberi perlakuan dengan kayu apu. Hasil uji statistik dengan uji Kruskal Wallis menunjukkan nilai p= 0,280 sehingga disimpulkan tidak ada perbedaan penyerapan Cr yang terdapat pada limbah batik pada jenis tanaman enceng gondok, kayu apu, dan kayambang.

Kata Kunci : Cr, Limbah batik, Fitoremediasi

Abstract
Batik motifs and colors are produced with dyes containing heavy metal chromium (Cr). Batik industries is generally classified as small and medium enterprises, so most of them do not process batik waste containing heavy metal Cr. The purpose of this research was to provide alternative solution from Cr pollution impact by utilizing various plants to absorb Cr in batik waste. This research was a quasi experiment with pre and post test design. The selected aquatic plants were water hyacinth or “enceng gondok” (Eichornia crassipes), “kayu apu” (Pistia stratiotes), and “kayambang” (Salvinia cucullata). They were grown in containers containing waste water for 5 days. The Cr concentration in wastewater of group 1 decreased from 0.0546 mg/l to 0.0378 mg/l after being treated with water hyacinth. The Cr concentration in wastewater of group 2 decreased from group 2 from 0.0488 mg/l to 0.0315 mg/l after being treated with “kayambang”. The Cr concentration in wastewater of group 3 decreased from 0.0464 mg/l to 0.0240 mg/l after being treated with “kayu apu”. Statistical test with Kruskal Wallis test showed that p = 0,280. It was concluded that there were no difference in Cr absorbtion among water hyacinth, kayambang and kayu apu.
KORELASI PENGETAHUAN, KEPUASAN, MOTIVASI DENGAN KONSISTENSI PEMAKAIAN KONDOM PADA PELANGGAN WPS DI SUNAN KUNING
Authors: Wirastri Dwi , Deliana Sri Maryati , Mukaromah Siti Baitul 
Published: September 2017

Data profil kesehatan kota Semarang tahun 2014 menunjukkan kasus IMS mengalami peningkatan selama 5 tahun terakhir, tahun 2010 sejumlah 2493 dan tahun 2014 sebanyak 5749 kasus. Penggunaan kondom yang rendah mengakibatkan tingginya prevalensi IMS. Tujuan penelitian untuk menganalisis pengetahuan, kepuasan, motivasi dengan konsistensi pemakaian kondom untuk mencegah penularan IMS pada Pelanggan. Metode penelitian ini adalah kuantitatif dengan desain cross sectional. Sampel berjumlah 30 dengan teknik insidental sampling. Hasil uji Chi Square nilai p=0,593 yaitu tidak ada hubungan antara pengetahuan dengan konsistensi pemakain kondom. hasil uji Chi Square nilai p=0,166 yaitu tidak ada hubungan antara kepuasan dengan konsistensi pemakain kondom. uji Chi Square nilai p=0,015 yaitu ada hubungan antara motivasi dengan konsistensi pemakain kondom. multivariat menunjukan bahwa Motivasi memiliki p< 0,05. Saran yang peneliti rekomendasikan adalah sosialisasi pemberian informasi pentingnya penggunaan kondom pada pelanggan WPS untuk menekan angka kejadian IMS.

Kata kunci : pengetahuan, kepuasan, motivasi, konsistensi pemakaian kondom

ABSTRACT
Health profile in Semarang city data in 2014 showed cases of STIs have increased over the last 5 years, in 2010 a number of 2493 and 2014 as many as 5749 cases. Low condom use resulted in a high prevalence of STIs. The aim of research to analyze knowledge, satisfaction, motivation with the consistency of the use of condoms to prevent the transmission of STIs on customers. This research method is quantitative with cross sectional design. Samples numbered 30 with incidental sampling technique. The result of Chi Square test p= 0.593 there is no correlation between knowledge and consistency of condom usage. the results of Chi Square test p-value = 0.166 there is no relationship between satisfaction with the consistency of condom usage. Chi square test p = 0.015 there is a relationship between motivation and consistency of condom usage. Multivariate showed that motivation has a p <0.05. Suggestions researchers recommend is the provision of information dissemination on the importance of condom use WPS customers to suppress the incidence of STIs.
Keywords : knowledge , satisfaction , motivation , use condoms consistency
ID:4005 Injectable Cell Delivery Constructs for Myocardial Tissue Engineering
Authors: Sung Hsing-Wen , Huang Chieh-Cheng , Chen Ding-Yuan , Hsiao Chun-Wen , Lee Wen-Yu , Chang Yen 
Published: September 2017

Cell transplantation via direct intramuscular injection is a promising therapy for patients with ischemic diseases. However, following injections, retention of transplanted cells in engrafted areas remains problematic, and can be deleterious to cell-transplantation therapy. In this presentation, a thermo-responsive hydrogel system composed of aqueous methylcellulose (MC) blended with phosphate-buffered saline is constructed to grow cell sheet fragments and cell bodies for the treatment of ischemic diseases. The as-prepared MC hydrogel system undergoes a sol–gel reversible transition upon heating or cooling at approximately 32 °C. Via this unique property, the grown cell sheet fragments (cell bodies) can be harvested without using proteolytic enzymes; consequently, their inherent extracellular matrices (ECMs) and integrative adhesive agents remain well preserved. In animal studies using rats and pigs with experimentally created myocardial infarction, the injected cell sheet fragments (cell bodies) become entrapped in the interstices of muscular tissues and adhere to engraftment sites, while a minimal number of cells exist in the group receiving dissociated cells. Moreover, transplantation of cell sheet fragments (cell bodies) significantly increases vascular density, thereby improving the function of an infarcted heart.
These experimental results demonstrate that cell sheet fragments (cell bodies) function as a cell-delivery construct by providing a favorable ECM environment to retain transplanted cells locally and consequently, improving the efficacy of therapeutic cell transplantation
ID:4007 Immune-cell base for cancer therapy
Authors: Ta Van Thanh , Tran Thinh Huy , Nguyen Binh Thanh , Nguyen Linh Quy , Nguyen Hoai Quy , Tran Khanh Van 
Published: September 2017

The development of immune cell-based approaches for treatment of cancer has been actively investigated for many years. One strategy that has been demonstrated as an effective method for cancer treatment is adoptive T cell therapy. The principle of this method is using Cytotoxic T lymphocytes (CTL), a crucial component of the adaptive immune system that aids in the control of intracellular pathogens. Effector CTL have the capacity to promote the apoptotic death of specifically targeted cells, using a combination of granule (perforin/granzyme)-and receptor (Fas/tumor necrosis factor)-mediated mechanisms. CTL recognize specific antigen on target cells using an unique T-cell receptor (TCR) when they are presented by class I major histocompatibility (MHC) molecules. In this study, we demonstrated that T lymphocytes were activated and dramatically expanded by stimulation with anti-CD3/CD28 antibodies and culture in the present of IL-2, IL-15 and IL-21 cytokines. These T cells exhibited a predominantly activated phenotype as manifested by an increase in the percentage of cells expressing CD8 and generation of various cytokines such as IL-2, INFγ and TNFa. These findings indicate that stimulation by anti- CD3/CD28 generated effector CTL in adoptive T-cell therapy for cancer.
ID:3001 3D bone grafting techniques in reconstruction of atrophic alveolar ridge in combination with implant placement
Authors: Vo Nhan Van , Lam Phong Dai , Truong Bao Chi , Trinh Tri Quang , Ta Quan Dong 
Published: September 2017

So far, atrophic alveolar ridge defect has been remained a big challenge for implant surgeons. Correct technique indication and proper material selection plays an important role for the long term success and further complications prevention. Numerous donor site for bone reconstruction prior and during implant placement have been proposed such as autogenous, homogenous allogenous or synthetic marterial. Among them, autologous graft is considered the “gold standard” as it meet three factors for the bone augmentation’s success due to its osteogenic, osteoinductive, and osteoconductive properties that others donor site could not obtain.
In this report, we would like to present the clinical results, the advantages and disadvantages, as well as the complications of different 3D bone grafting techniques and materials in reconstruction of severely atrophic maxilla and mandible in implant rehabilitation.
ID:3003 Increase in CD34 cells in peripheral blood following HLA randomized, ABO-matched cord blood transfusion in patients with advanced malignancy and anemia: Prognostic implications
Authors: Bhattacharya Niranjan 
Published: September 2017

Cord blood is a safe alternative of adult blood for transfusion given its content of fetal and adult haemoglobin, platelet and WBC counts, and a plasma filled with inflammatory and non-inflammatory cytokines and growth factors. It also has a hypoantigenic nature and altered metabolic profile and is rich in haematopoietic stem cells (CD34) . It has potential for treatment of anemia in diseases of different aetiologies including malignancy.
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STUDY DESIGN:
In the present study, 46 sex and HLA-randomized patients suffering from advanced malignancy and anemia (Hb 8 Gm percent or less) were treated with placental cord blood transfusion. CD34 cells were collected from peripheral blood 72 hours after transfusion and variations were analyzed for prognostic implication. The study period was between 16 August 1999 and 16 May 2006.
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RESULTS AND ANALYSIS:
Among the forty-six cases included in this study , Case no 2 (breast sarcoma) received the lowest amount of card blood (6 units), while Case no 46 (breast cancer) received the largest amount (32 units). The youngest patient, suffering from non-Hodgkin's lymphoma (Case 3), was a 16-year-old boy who received eight units of cord blood to combat anemia. Other patients received amounts varying from 7-15 units: Case 14 received 15 units (metachronous lymph node metastatsis), Case 19 received 14 units (breast cancer), and Case 25 received seven units (lung cancer).
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There was no transfusion-related visible immunological or nonimmunological reaction in any patient. Studies of CD34 levels after 72 hours of transfusion showed an initial rise followed by a fall in twenty-two cases, two cases registered very little effect on the CD34 level, i.e., no change from the baseline, and the rest of the cases demonstrated a sustained rise from the baseline, i.e, 56% to 99% of peripheral mononuclear cells and a sustained high CD34 level.
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CONCLUSION:
This preliminary study indicates that freshly collected cord blood transfusion may cause a transient transplant impact of transfused cord blood CD34 stem cells on the host without provoking clinical graft vs host disease perhaps due to a possible background structural or functional immune suppression in advanced malignancy.
From the cord blood transfusion impact we could clinically divide the enrolled advanced cancer patients to a relatively good (Group A) and bad prognostic(Group B) variety of cancer. The survival rate of patients where we detected a persistant rise of CD34 in peripheral blood after 3 units or more of transfusion of HLA randomized ABO matched and screened cord blood was over 3 years and the cases where there are no rise of peripheral blood CD34 level in spite of repeated transfusion of 5- 32 units of cord blood died within 3 months or earlier (p value less than .01)
ID3012 Evaluation of HLA-identical sibling allogenic peripheral blood stem cell transplantation for acute myelogenous leukemia at BTH, Vietnam
Authors: Huynh Man Van 
Published: September 2017

Background: the purposes of this study were to assess the efficacy of HLA-identical sibling allogenic peripheral blood stem cell transplantation in the treatment for acute myelogenous leukemia (AML) at Blood transfusion and hematology hospital at Ho Chi Minh city.

Methods: retrospective study, case series.

Results: from June 2005 to September 2016, there were 47 AML patients included 24 males and 23 females, with median age was 36 years old. Engraftment was achieved in 47 patients (100%). The median number of transfused CD34+ cells was 7.5 x 106/kg. The median time for neutrophil engraftment and platelet engraftment were 11 ± 2.8 days and 12.5 ± 10.1 days respectively. Oral mucositis occurred in 38 patients (80.9%), febrile neutropenia in 42 patients (89.4%), acute graft-versus-host disease (aGvHD) in 14 patients (29.8%), chronic graft-versus-host disease (cGvHD) in 10 patients (21.3%), and VOD in 6 patients (12.8%). Transplant-related mortality (TRM) was 4 patients (8.5%). Median follow-up time was 52.26 months. Patients remained alive were 27 (57.4%). The 5-year overall survival (OS) and disease-free survival (DFS) were 55% and 54% respectively.

Conclusion: HLA-identical sibling allogeneic blood stem cell transplantation can reconstruct hematopoiesis quickly and is a favorable therapeutic method for AML patients. Further development and improvement of hematopoietic stem cell transplantation techniques along with graduated and individualized therapy are expected to provide better outcomes and improved quality of life for AML patients.
ID3015 Mesenchymal stem cells for treatment of chronic wounds: A Study with autologous transplantation of adipose-derived stem cells
Authors: Dinh Han Van 
Published: September 2017

Objective: This study was to determine the effects of adipose-derived stem cells (ADSCs) on dermal fibroblasts responses to injury including migration and proliferation in vitro. We also evaluated the autologous transplantation of ADSCs on treatment of human chronic wounds.
Subjects and methods: The proliferation and migration of fibroblast was evaluated by co-culture ADSCs with allogenic dermal fibroblast and by the scratch assay. In clinical study, autologous ADSCs were transplanted on to chronic wounds of 25 patients, who were hospitalized into the Wound Healing Department of the National Institute of Burns from April, 2015 to June, 2016. The mean age was 56.88 ± 16.81, male/female ratio was 2.12. The autologous adipose-derived stem cells at passages 5 were transplanted on surface of wound every 3÷5 days. The wound biopsies for H&E staining and for Transmission Electron Microscope were taken before transplantation and at day 7, day15 and day 20 of studied progress.
Results: ADSCs stimulated fibroblast proliferation and migration in wound healing assay. In clinical study, before transplantation, extracellular matrix (ECM) was destroyed. After transplantation, ADSCs strongly stimulated fibroblast proliferation and fibroblasts to produce collagen. ADSCs also promoted proliferations of epithelial cells and neovascularization at the chronic wound site.
Conclusion: Autologous ADSCs promoted the wound healing process by cell proliferation and improvement of ECM in chronic wound site.
ID3021 Acquired resistance to with EGFR tyrosine kinase inhibitors by the EGFR T790M mutation in non-small cell lung cancer
Authors: Ta Van Thanh 
Published: September 2017

Mutations in the tyrosine kinase (TK) domain of the epidermal growth factor receptor (EGFR) gene respond well to treatment with EGFR tyrosine kinase inhibitors (EGFR-TKIs) including gefitinib and erlotinib in non-small cell lung cancer (NSCLC). Detection of these mutations has an important role for therapeutic decision-making in NSCLC treatment. However, all patients who experienced marked improvements with these drugs eventually developed disease progression after 10-20 months of treatment due to the acquisition of drug resistance. Approximately half of the cases with acquired resistance to EGFR-TKIs can be accounted for by a second-site mutation in exon 20 of the EGFR kinase domain T790M. In this study, 40 patients with advanced NSCLC who developed acquired resistance to EGFR-TKIs were selected. The diagnosis was defined based on the Jackman criteria for acquired resistance to EGFR-TKIs in lung cancers. Re-biopsy were performed at National cancer hospital and Oncology hospitals at Ha Noi and Ho Chi Minh City. Scorpion ARMS method was used to detect EGFR mutation status. In all, 40% (16/40) of the patients carried T790M mutation after the failure of EGFR-TKIs. The study demonstrated a critical role of molecular diagnostics for TKI acquired resistance through re-biopsies at the time of disease progression.
ID2006 Crosstalk between effector cells of adaptive immunity and mesenchymal stromal cells
Authors: Gornostaeva Aleksandra 
Published: September 2017

Multipotent mesenchymal stromal cells (MSCs) are a perspective tool for regenerative medicine due low immunogenicity and immunomodulation. The "feedback loop" exists in MSC/immune cells relationships, when "inflammatory" stimulation switches immunoregulaton by MSCs. Currently, the most studied effect of allogeneic MSCs on adaptive immunity cells, mainly on T lymphocytes. Studies of the interaction of MSCs and innate immunity cells are much less. "Reverse effects" (the effect of immune cells on MSCs) are virtually not investigated. Initiation of the inflammation occurs with activation of innate immunity cells, that "turns on" immunomodulatory properties. In this regard, the study of the interaction of MSCs and monocytes is particularly relevant. MSCs from human adipose tissue and CD14+monocytes (MNs) from peripheral blood of healthy volunteers were used. To stimulate monocytes conditioned medium (CM) after 72 hours of mixed lymphocyte reaction (MLR) was applied. This CM was enriched with IL-8, INF-gamma and TNF-a. Optimization of MN activation procedure was performed prior to experiments. CD14+MNs were incubated with different concentration of MLR-CM for a different time. The activation and viability of MNs was evaluated every 24 hours. The overnight exposure of MNs to 3-day 50% CM-MLR was found to be optimum regime. We studied the of MSC/monocyte interaction paying special attention to "feedback loop". In the presence of activated MNs, MSCs possessed unchanged viability (96%), transmembrane mitochondria potential, ROS level and twice reduced lysosome activity. The cytokine profile in coculture medium was changed significantly. IL-6 and MCP-1 were increased vs monocultures of both cell types. IL-8 was similar to MN monoculture. TNF alpha, MIG, IL-10 were detected as tracers. Elevation of IL-6 and MIG indicates on acquisition of anti-inflammatory phenotype by MSCs. After interaction with MSC, the share of CD69+ MNs (nonspecific marker of early activation) decreased, HLA-DR (MHC class II receptor) increased slightly. A threefold increase in both CD163+ MN’s share and MFI was detected, whereas CD86 antigen was not expressed. The changes in the cytokine profile and the expression of surface markers described above are characteristic of the anti-inflammatory phenotype of monocytes. Thus, upon interaction MSC exhibited pronounced immunomodulatory properties and shifted the phenotype of monocytes towards the anti-inflammatory. These data indicate on the MSC potential to modulate early stages of inflammation, while retaining their functional state.
ID2009 H. Odorata methanol extract inhibits hepatocellular carcinoma HepG2 cells line via induction of caspase-dependent apoptosis
Authors: Nguyen Sinh Truong 
Published: September 2017

Background: Globally cancer is a disease which is major burden to human health nowadays. The demand for new therapies to treat and to prevent cancer disease frequently exists. Since the toxic side effect of current treatment such as chemotherapy, the research interest is paying attention toward naturally derived-compounds because of their selective toxicity to cancer cell. This study aims to test the anticancer activity of a crude extract of Hopea odorata on HepG2 cancer cell line.

Method: Methanol extract were prepared from the bark of H. odorata plant. In vitro cytotoxicity Hopea odorata extract on human hepatocellular carcinoma cell line HepG2, compared to normal human cell fibroblast (HF), was investigated by Alamar Blue assay. Caspase-3/7 was detected using the reagent that consists of DEVD peptide conjugated to a nucleic acid-binding dye. The apoptosis induction of plant extract on HepG2 was recognized by Annexin V/7AAD using flow cytometry. Disintergrated nuclei of plant-treated cell was observed under fluorescent microscope using Hoechst/PI staining. With the same technique of staining, the ratio of dead/total cells was determined by distingusing Hoechst and PI fluorescent signal.

Results. We found that the IC50 value of Hopea odorata extract on HepG2 is at 12.67± 5µg/ml, this value is at 44.2± 3µg/ml on HF. Vice versa, the IC50 value of Doxo on HepG2 153.3±15ng/ml while that is 6.3 ±0.6083ng/ml on HF. The selectivity index of H. Odorata extract (SI) toward HepG2 cells is approximately 3.48, while the SI of Doxorubicin toward HepG2 cells is approximately 0.04. The ratio of dead/total cells increased in dose-dependent manner when observed under fluorescent microscope, while the ratio of dead/total cells barely changed on HF cells. The plant extract inhibited HepG2 through the activation of caspase-3/7. At the concentration 250µg/ml of plant extract, 35% HepG2 cells was induced into apoptosis, and HepG2 cells appeared with disintegrated nuclei under fluorescent microscope.
Conclusion. These finding showed methanolic extract of Hopea odorata plant induced apoptosis and selectively cytotoxic toward HepG2 rather than human fibroblast cells. Purification of compounds from Hopea odorata extract need to be performed for further research the anticancer properties of Hopea odorata.