bluuebunny/crossref_metadata_2025_split huggingface dataset:
Finger Trauma Due to Surfing; A Case Series and Analysis of Fracture Patterns
Authors: Ruijs A.C.J. , Langenberg L.C. , Rezzouk J. 
Published: February 2017

Background: The purpose of this study is to research the type of lesions to the hand and fingers in the sport of surfing.Methods: All surfing related hand injuries reported to our hospital between January 2008 and September 2015 were analyzed.Results: Thirty-seven patient files held a clear description of a trauma related to the surf sport. We found three finger sprains, five wounds needing suture, including one digital nerve lesion and one ring finger with flexor tendon injury, three fingertip amputations and twenty-six digital fractures. The fracture type was similar in twenty-one patients: an open extra-articular transverse fracture of the distal phalanx. Thirty-one injuries were caused by the surf leash.Conclusions: From this case series we can conclude that surfing may lead to significant trauma to the fingers, mainly due to the leash. This can be caused by grasping the leash while it snaps to tension, which may lead to hyper flexion of the distal phalanx, resulting in a typical trans-phalangeal fracture. Also, when the leash is wrapped around a finger or grasped near the attachment of the leash to the board, ring avulsion-like trauma may occur, leading to open fractures or (partial) amputations. Recognizing that surf leash trauma causes a particular type of fracture to the distal phalanx, may lead to better education of surfers and the development of safer surfing equipment.
Vascularized Medial Femoral Condyle Bone Graft for Resistant Nonunion of the Distal Radius
Authors: Henry Mark 
Published: February 2017

Background: Nonunion involving the metaphyseal region of the distal radius is exceedingly rare, usually involving co-morbidity. Patients that have failed multiple prior conventional surgical interventions represent an even more difficult subset to treat; this investigation examined the utility of a specially designed free vascularized medial femoral condyle flap consisting of a central structural block graft with an extended corticoperiosteal sleeve to wrap around the junctions. Methods: Six patients (5 males, 1 female) with a mean age of 52 years had failed to achieve union involving the distal radius metaphysis after a mean of 3.7 prior surgeries occurring over a mean period of 24 months. Comorbidities included smoking, alcoholism, chronic nutritional deficiency, and prior osteomyelitis. The unique descending genicular artery medial femoral condyle flap designed to address these patients consisted of a central structural block graft in continuity with an extended corticoperiosteal sleeve. The structural block filled the bone defect, and the corticoperiosteal sleeve wrapped around the bone junctions and the neighboring bone margins. The mean flap size was 5.3 (+/- 1.3) cm long by 4.5 (+/- 0.9) cm wide. Pre-operative to post-operative DASH scores were compared using the paired student’ s t-test, with p < 0.05. Results: All flaps achieved union at a mean of 6.8 (+/- 2.1) weeks following surgery, using the criteria of bridging trabeculae on all 3 radiographs: coronal, sagittal, and oblique. The mean pre-operative DASH score of 63 (+/- 10) was statistically significantly different compared to the mean post-operative DASH score of 18 (+/- 8). Conclusions: With few alternative solutions able to address this unique and difficult problem, the structural block of vascularized bone with the extended corticoperiosteal sleeve proved able to achieve a union that had failed multiple previous attempts and able to resist reactivation of infection, in a challenging group of patients with comorbidities.
A New Classification Scheme for Closed Avulsion Injuries of the Flexor Digitorum Profundus Tendon
Authors: Azeem Mokhtar Abdul , Marwan Yousef , Morshidy Ahmed El , Esmaeel Ali , Zakaria Yehia 
Published: February 2017

Background: Closed avulsion injury of the flexor digitorum profundus (FDP) tendon is a relatively common condition of the hand. Its present classification system seems to be deficient in including all possible patterns of injury, leading to improper selection of the best treatment method for the injury. We aim to provide a new classification scheme for this injury.Methods: We developed a new classification scheme based on the possible pattern of FDP tendon injury. It consisted of three main types, and 10 sub-types. This was used to classify the injury of 34 patients, and help in choosing the best management approach. All patients underwent surgical treatment of their injury. The distal interphalangeal (DIP) joint extension deficit and total active motion (TAM) of the proximal interphalangeal (PIP) and DIP joints were considered as the main outcomes. Five orthopaedic surgeons used the new classification system for our cases, and the inter-rater reproducibility was tested with Fleiss' kappa.Results: The multi-rater kappa for the classification was excellent. At the final follow-up visit 24 months following surgery, the mean loss of extension of the DIP joint was 13.76° ± 13.53° (range 0° to 45°), while the mean TAM was 148.88° ± 22.64° (range 94° to 172°). Based on the TAM score, 21 (61.76%), 8 (23.53%) and 5 (14.71%) patients had excellent, good and fair results, respectively. None of our patients had poor results.Conclusions: Our new classification scheme of FDP tendon avulsion appeared to be comprehensive and useful in guiding the surgeon for the best treatment option. Nevertheless, this should be confirmed by using it for larger number of patients with different patterns of injury.
An Experimental Survey on the Effect of Using the CTS-6 Tool on the Diagnosis of Carpal Tunnel Syndrome by Hand Surgeons
Authors: Hutchison Richard L. , Hutchison Alan L. , Hirthler Maureen A. 
Published: February 2017

Background: This controlled randomized experiment tested the research hypothesis that providing the CTS-6 quantitative diagnostic information to hand surgeons affects the diagnosis of carpal tunnel syndrome.Methods: Surgeon members of American Association for Hand Surgery participated in an online survey. Demographic and practice pattern information was collected. Few surgeons routinely use diagnostic questionnaires or algorithms. Each member was given four clinical scenarios. The respondents were randomized, The experimental group was given the same scenarios as the control group plus the quantitative results of the CTS-6 diagnostic tool.Results: There were statistically significant differences between the groups in the diagnostic decisions. Using the CTS-6 quantitative diagnostic tool affected the diagnosis of carpal tunnel syndrome, especially for patients with the lowest number of findings associated with carpal tunnel syndrome.Conclusions: While accurate diagnostic decisions are dependent on the incorporation of all of the pertinent information gathered during the history and physical exams, the results of the CTS-6 may help the clinician focus their thinking and revise their diagnostic probabilities.
The influence of rosuvastatin on the gastrointestinal microbiota and host gene expression profiles
Authors: Nolan J. A. , Skuse P. , Govindarajan K. , Patterson E. , Konstantinidou N. , Casey P. G. , MacSharry J. , Shanahan F. , Stanton C. , Hill C. , Cotter P. D. , Joyce S. A. , Gahan C. G. M. 
Published: February 2017

Statins are the most widely prescribed medications worldwide for the treatment of hypercholesterolemia. They inhibit the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-R), an enzyme involved in cholesterol synthesis in higher organisms and in isoprenoid biosynthesis in some bacteria. We hypothesized that statins may influence the microbial community in the gut through either direct inhibition or indirect mechanisms involving alterations to host responses. We therefore examined the impact of rosuvastatin (RSV) on the community structure of the murine gastrointestinal microbiota. RSV was orally administered to mice and the effects on the gut microbiota, host bile acid profiles, and markers of inflammation were analyzed. RSV significantly influenced the microbial community in both the cecum and feces, causing a significant decrease in α-diversity in the cecum and resulting in a reduction of several physiologically relevant bacterial groups. RSV treatment of mice significantly affected bile acid metabolism and impacted expression of inflammatory markers known to influence microbial community structure (including RegIIIγ and Camp) in the gut. This study suggests that a commonly used statin (RSV) leads to an altered gut microbial composition in normal mice with attendant impacts on local gene expression profiles, a finding that should prompt further studies to investigate the implications of statins for gut microbiota stability and health in humans. NEW & NOTEWORTHY This work demonstrates that rosuvastatin administration in mice affects the gastrointestinal microbiota, influences bile acid metabolism, and alters transcription of genes encoding factors involved in gut homeostasis and immunity in the gastrointestinal tract.
Cardiorespiratory fitness modulates the acute flow-mediated dilation response following high-intensity but not moderate-intensity exercise in elderly men
Authors: Bailey Tom G. , Perissiou Maria , Windsor Mark , Russell Fraser , Golledge Jonathan , Green Daniel J. , Askew Christopher D. 
Published: February 2017

Impaired endothelial function is observed with aging and in those with low cardiorespiratory fitness (V̇o2peak). Improvements in endothelial function with exercise training are somewhat dependent on the intensity of exercise. While the acute stimulus for this improvement is not completely understood, it may, in part, be due to the flow-mediated dilation (FMD) response to acute exercise. We examined the hypothesis that exercise intensity alters the brachial (systemic) FMD response in elderly men and is modulated by V̇o2peak. Forty-seven elderly men were stratified into lower (V̇o2peak = 24.3 ± 2.9 ml·kg−1·min−1; n = 27) and higher fit groups (V̇o2peak = 35.4 ± 5.5 ml·kg−1·min−1; n = 20) after a test of cycling peak power output (PPO). In randomized order, participants undertook moderate-intensity continuous exercise (MICE; 40% PPO) or high-intensity interval cycling exercise (HIIE; 70% PPO) or no-exercise control. Brachial FMD was assessed at rest and 10 and 60 min after exercise. FMD increased after MICE in both groups {increase of 0.86% [95% confidence interval (CI), 0.17–1.56], P = 0.01} and normalized after 60 min. In the lower fit group, FMD was reduced after HIIE [reduction of 0.85% (95% CI, 0.12–1.58), P = 0.02] and remained decreased at 60 min. In the higher fit group, FMD was unchanged immediately after HIIE and increased after 60 min [increase of 1.52% (95% CI, 0.41–2.62), P < 0.01, which was correlated with V̇o2peak, r = 0.41; P < 0.01]. In the no-exercise control, FMD was reduced in both groups after 60 min ( P = 0.05). Exercise intensity alters the acute FMD response in elderly men and V̇o2peak modulates the FMD response following HIIE but not MICE. The sustained decrease in FMD in the lower fit group following HIIE may represent a signal for vascular adaptation or endothelial fatigue. NEW & NOTEWORTHY This study is the first to show that moderate-intensity continuous cycling exercise increased flow-mediated dilation (FMD) transiently before normalization of FMD after 1 h, irrespective of cardiorespiratory fitness level in elderly men. Interestingly, we show increased FMD after high-intensity cycling exercise in higher fit men, with a sustained reduction in FMD in lower fit men. The prolonged reduction in FMD after high-intensity cycling exercise may be associated with future vascular adaptation but may also reflect a period of increased cardiovascular risk in lower fit elderly men.
AAV-mediated delivery of optogenetic constructs to the macaque brain triggers humoral immune responses
Authors: Mendoza Skyler D. , El-Shamayleh Yasmine , Horwitz Gregory D. 
Published: February 2017

Gene delivery to the primate central nervous system via recombinant adeno-associated viral vectors (AAV) allows neurophysiologists to control and observe neural activity precisely. A current limitation of this approach is variability in vector transduction efficiency. Low levels of transduction can foil experimental manipulations, prompting vector readministration. The ability to make multiple vector injections into the same animal, even in cases where successful vector transduction has already been achieved, is also desirable. However, vector readministration has consequences for humoral immunity and gene delivery that depend on vector dosage and route of administration in complex ways. As part of optogenetic experiments in rhesus monkeys, we analyzed blood sera collected before and after AAV injections into the brain and quantified neutralizing antibodies to AAV using an in vitro assay. We found that injections of AAV1 and AAV9 vectors elevated neutralizing antibody titers consistently. These immune responses were specific to the serotype injected and were long lasting. These results demonstrate that optogenetic manipulations in monkeys trigger immune responses to AAV capsids, suggesting that vector readministration may have a higher likelihood of success by avoiding serotypes injected previously.NEW & NOTEWORTHY Adeno-associated viral vector (AAV)-mediated gene delivery is a valuable tool for neurophysiology, but variability in transduction efficiency remains a bottleneck for experimental success. Repeated vector injections can help overcome this limitation but affect humoral immune state and transgene expression in ways that are poorly understood. We show that AAV vector injections into the primate central nervous system trigger long-lasting and serotype-specific immune responses, raising the possibility that switching serotypes may promote successful vector readministration.
Prolactin Receptor–Mediated Internalization of Imaging Agents Detects Epithelial Ovarian Cancer with Enhanced Sensitivity and Specificity
Authors: Sundaram Karthik M. , Zhang Yilin , Mitra Anirban K. , Kouadio Jean-Louis K. , Gwin Katja , Kossiakoff Anthony A. , Roman Brian B. , Lengyel Ernst , Piccirilli Joseph A. 
Published: February 2017

Abstract
Poor prognosis of ovarian cancer, the deadliest of the gynecologic malignancies, reflects major limitations associated with detection and diagnosis. Current methods lack high sensitivity to detect small tumors and high specificity to distinguish malignant from benign tissue, both impeding diagnosis of early and metastatic cancer stages and leading to costly and invasive surgeries. Tissue microarray analysis revealed that &gt;98% of ovarian cancers express the prolactin receptor (PRLR), forming the basis of a new molecular imaging strategy. We fused human placental lactogen (hPL), a specific and tight binding PRLR ligand, to magnetic resonance imaging (gadolinium) and near-infrared fluorescence imaging agents. Both in tissue culture and in mouse models, these imaging bioconjugates underwent selective internalization into ovarian cancer cells via PRLR-mediated endocytosis. Compared with current clinical MRI techniques, this targeted approach yielded both enhanced signal-to-noise ratio from accumulation of signal via selective internalization and improved specificity conferred by PRLR upregulation in malignant ovarian cancer. These features endow PRLR-targeted imaging with the potential to transform ovarian cancer detection. Cancer Res; 77(7); 1684–96. ©2017 AACR.
Myeloid Cells That Impair Immunotherapy Are Restored in Melanomas with Acquired Resistance to BRAF Inhibitors
Authors: Steinberg Shannon M. , Shabaneh Tamer B. , Zhang Peisheng , Martyanov Viktor , Li Zhenghui , Malik Brian T. , Wood Tamara A. , Boni Andrea , Molodtsov Aleksey , Angeles Christina V. , Curiel Tyler J. , Whitfield Michael L. , Turk Mary Jo 
Published: February 2017

Abstract
Acquired resistance to BRAFV600E inhibitors (BRAFi) in melanoma remains a common clinical obstacle, as is the case for any targeted drug therapy that can be developed given the plastic nature of cancers. Although there has been significant focus on the cancer cell-intrinsic properties of BRAFi resistance, the impact of BRAFi resistance on host immunity has not been explored. Here we provide preclinical evidence that resistance to BRAFi in an autochthonous mouse model of melanoma is associated with restoration of myeloid-derived suppressor cells (MDSC) in the tumor microenvironment, initially reduced by BRAFi treatment. In contrast to restoration of MDSCs, levels of T regulatory cells remained reduced in BRAFi-resistant tumors. Accordingly, tumor gene expression signatures specific for myeloid cell chemotaxis and homeostasis reappeared in BRAFi-resistant tumors. Notably, MDSC restoration relied upon MAPK pathway reactivation and downstream production of the myeloid attractant CCL2 in BRAFi-resistant melanoma cells. Strikingly, although combination checkpoint blockade (anti-CTLA-4 + anti-PD-1) was ineffective against BRAFi-resistant melanomas, the addition of MDSC depletion/blockade (anti-Gr-1 + CCR2 antagonist) prevented outgrowth of BRAFi-resistant tumors. Our results illustrate how extrinsic pathways of immunosuppression elaborated by melanoma cells dominate the tumor microenvironment and highlight the need to target extrinsic as well as intrinsic mechanisms of drug resistance. Cancer Res; 77(7); 1599–610. ©2017 AACR.
Evolution of Cancer Stem-like Cells in Endocrine-Resistant Metastatic Breast Cancers Is Mediated by Stromal Microvesicles
Authors: Sansone Pasquale , Berishaj Marjan , Rajasekhar Vinagolu K. , Ceccarelli Claudio , Chang Qing , Strillacci Antonio , Savini Claudia , Shapiro Lauren , Bowman Robert L. , Mastroleo Chiara , De Carolis Sabrina , Daly Laura , Benito-Martin Alberto , Perna Fabiana , Fabbri Nicola , Healey John H. , Spisni Enzo , Cricca Monica , Lyden David , Bonafé Massimiliano , Bromberg Jacqueline 
Published: February 2017

Abstract
The hypothesis that microvesicle-mediated miRNA transfer converts noncancer stem cells into cancer stem cells (CSC) leading to therapy resistance remains poorly investigated. Here we provide direct evidence supporting this hypothesis, by demonstrating how microvesicles derived from cancer-associated fibroblasts (CAF) transfer miR-221 to promote hormonal therapy resistance (HTR) in models of luminal breast cancer. We determined that CAF-derived microvesicles horizontally transferred miR-221 to tumor cells and, in combination with hormone therapy, activated an ERlo/Notchhi feed-forward loop responsible for the generation of CD133hi CSCs. Importantly, microvesicles from patients with HTR metastatic disease expressed high levels of miR-221. We further determined that the IL6–pStat3 pathway promoted the biogenesis of onco-miR-221hi CAF microvesicles and established stromal CSC niches in experimental and patient-derived breast cancer models. Coinjection of patient-derived CAFs from bone metastases led to de novo HTR tumors, which was reversed with IL6R blockade. Finally, we generated patient-derived xenograft (PDX) models from patient-derived HTR bone metastases and analyzed tumor cells, stroma, and microvesicles. Murine and human CAFs were enriched in HTR tumors expressing high levels of CD133hi cells. Depletion of murine CAFs from PDX restored sensitivity to HT, with a concurrent reduction of CD133hi CSCs. Conversely, in models of CD133neg, HT-sensitive cancer cells, both murine and human CAFs promoted de novo HT resistance via the generation of CD133hi CSCs that expressed low levels of estrogen receptor alpha. Overall, our results illuminate how microvesicle-mediated horizontal transfer of genetic material from host stromal cells to cancer cells triggers the evolution of therapy-resistant metastases, with potentially broad implications for their control. Cancer Res; 77(8); 1927–41. ©2017 AACR.
MYC Mediates Large Oncosome-Induced Fibroblast Reprogramming in Prostate Cancer
Authors: Minciacchi Valentina R. , Spinelli Cristiana , Reis-Sobreiro Mariana , Cavallini Lorenzo , You Sungyong , Zandian Mandana , Li Xiaohong , Mishra Rajeev , Chiarugi Paola , Adam Rosalyn M. , Posadas Edwin M. , Viglietto Giuseppe , Freeman Michael R. , Cocucci Emanuele , Bhowmick Neil A. , Di Vizio Dolores 
Published: February 2017

Abstract
Communication between cancer cells and the tumor microenvironment results in the modulation of complex signaling networks that facilitate tumor progression. Here, we describe a new mechanism of intercellular communication originating from large oncosomes (LO), which are cancer cell–derived, atypically large (1–10 μm) extracellular vesicles (EV). We demonstrate that, in the context of prostate cancer, LO harbor sustained AKT1 kinase activity, nominating them as active signaling platforms. Active AKT1 was detected in circulating EV from the plasma of metastatic prostate cancer patients and was LO specific. LO internalization induced reprogramming of human normal prostate fibroblasts as reflected by high levels of α-SMA, IL6, and MMP9. In turn, LO-reprogrammed normal prostate fibroblasts stimulated endothelial tube formation in vitro and promoted tumor growth in mice. Activation of stromal MYC was critical for this reprogramming and for the sustained cellular responses elicited by LO, both in vitro and in vivo in an AKT1-dependent manner. Inhibition of LO internalization prevented activation of MYC and impaired the tumor-supporting properties of fibroblasts. Overall, our data show that prostate cancer–derived LO powerfully promote establishment of a tumor-supportive environment by inducing a novel reprogramming of the stroma. This mechanism offers potential alternative options for patient treatment. Cancer Res; 77(9); 2306–17. ©2017 AACR.
Local and Global Cues in the Prosodic Realization of Broad and Narrow Focus in Bulgarian
Authors: Andreeva Bistra , Barry William John , Koreman Jacques 
Published: February 2017

AbstractIn this study, local and global prosodic cues for information structure are examined in the elicited production of six Bulgarian sentences. The sentences were produced in response to different questions, devised to prompt different focus realizations (broad focus and non-contrastive and contrastive narrow focus). Results show that speakers consistently differentiate broad and narrow focus by means of both local and global acoustic cues, by producing different pitch accent types on the nuclear syllable and reducing the ‘phonetic strength' of the default pre-nuclear accent in the narrow focus condition. Thus, the difference between the acoustic properties of the nuclear and the pre-nuclear accented syllables is smaller in the broad focus condition and greater in the narrow focus condition. Contrastive and non-contrastive narrow-focus accents are differentiated by local cues, i.e., by longer duration when the focus is early in the sentence and by global cues, i.e., by enhancing the tonal contrast between the nuclear prominence of CW2 and the pre-nuclear prominence of CW1 when the focus is late in the sentence.
Teresa Wennberg, Pioneer Video and Computer Artist: Early Experiences in Video and Computer Art
Authors: Wennberg Teresa 
Published: February 2017

Abstract
The author, a Swedish artist, arrived in Paris in the 1970s as a painter. She then began working with electronic images and was one of the first artists to produce video in France. Following the evolution of the technology very closely, she continued to develop 2D and 3D computer art, often presented as multimedia installations. Eventually, she began working with virtual reality. In this article, she delivers a personal recollection of the beginning of her artistic meandering, current through the time of writing.