bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zebrafish biosensor for toxicant induced muscle hyperactivity
Authors: Shahid Maryam , Takamiya Masanari , Stegmaier Johannes , Middel Volker , Gradl Marion , Klüver Nils , Mikut Ralf , Dickmeis Thomas , Scholz Stefan , Rastegar Sepand , Yang Lixin , Strähle Uwe 
Published: March 2016

AbstractRobust and sensitive detection systems are a crucial asset for risk management of chemicals, which are produced in increasing number and diversity. To establish an in vivo biosensor system with quantitative readout for potential toxicant effects on motor function, we generated a transgenic zebrafish line TgBAC(hspb11:GFP) which expresses a GFP reporter under the control of regulatory elements of the small heat shock protein hspb11. Spatiotemporal hspb11 transgene expression in the musculature and the notochord matched closely that of endogenous hspb11 expression. Exposure to substances that interfere with motor function induced a dose-dependent increase of GFP intensity beginning at sub-micromolar concentrations, while washout of the chemicals reduced the level of hspb11 transgene expression. Simultaneously, these toxicants induced muscle hyperactivity with increased calcium spike height and frequency. The hspb11 transgene up-regulation induced by either chemicals or heat shock was eliminated after co-application of the anaesthetic MS-222. TgBAC(hspb11:GFP) zebrafish embryos provide a quantitative measure of muscle hyperactivity and represent a robust whole organism system for detecting chemicals that affect motor function.
Decoherence spectroscopy with individual two-level tunneling defects
Authors: Lisenfeld Jürgen , Bilmes Alexander , Matityahu Shlomi , Zanker Sebastian , Marthaler Michael , Schechter Moshe , Schön Gerd , Shnirman Alexander , Weiss Georg , Ustinov Alexey V. 
Published: March 2016

AbstractRecent progress with microfabricated quantum devices has revealed that an ubiquitous source of noise originates in tunneling material defects that give rise to a sparse bath of parasitic two-level systems (TLSs). For superconducting qubits, TLSs residing on electrode surfaces and in tunnel junctions account for a major part of decoherence and thus pose a serious roadblock to the realization of solid-state quantum processors. Here, we utilize a superconducting qubit to explore the quantum state evolution of coherently operated TLSs in order to shed new light on their individual properties and environmental interactions. We identify a frequency-dependence of TLS energy relaxation rates that can be explained by a coupling to phononic modes rather than by anticipated mutual TLS interactions. Most investigated TLSs are found to be free of pure dephasing at their energy degeneracy points, around which their Ramsey and spin-echo dephasing rates scale linearly and quadratically with asymmetry energy, respectively. We provide an explanation based on the standard tunneling model, and identify interaction with incoherent low-frequency (thermal) TLSs as the major mechanism of the pure dephasing in coherent high-frequency TLS.
Design, synthesis, crystallization and biological evaluation of new symmetrical biscationic compounds as selective inhibitors of human Choline Kinase α1 (ChoKα1)
Authors: Schiaffino-Ortega Santiago , Baglioni Eleonora , Mariotto Elena , Bortolozzi Roberta , Serrán-Aguilera Lucía , Ríos-Marco Pablo , Carrasco-Jimenez M. Paz , Gallo Miguel A. , Hurtado-Guerrero Ramon , Marco Carmen , Basso Giuseppe , Viola Giampietro , Entrena Antonio , López-Cara Luisa Carlota 
Published: March 2016

AbstractA novel family of compounds derivative of 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))-bispyridinium or –bisquinolinium bromide (10a-l) containing a pair of oxygen atoms in the spacer of the linker between the biscationic moieties, were synthesized and evaluated as inhibitors of choline kinase against a panel of cancer-cell lines. The most promising compounds in this series were 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))bis(4-(dimethylamino)pyridinium) bromide (10a) and 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))-bis(7-chloro-4-(pyrrolidin-1-yl)quinolinium) bromide (10l), which inhibit human choline kinase (ChoKα1) with IC50 of 1.0 and 0.92 μM, respectively, in a range similar to that of the previously reported biscationic compounds MN58b and RSM932A. Our compounds show greater antiproliferative activities than do the reference compounds, with unprecedented values of GI50 in the nanomolar range for several of the cancer-cell lines assayed, and more importantly they present low toxicity in non-tumoral cell lines, suggesting a cancer-cell-selective antiproliferative activity. Docking studies predict that the compounds interact with the choline-binding site in agreement with the binding mode of most previously reported biscationic compounds. Moreover, the crystal structure of ChoKα1 with compound 10a reveals that this compound binds to the choline-binding site and mimics HC-3 binding mode as never before.
Autophagy Negatively Regulates Transmissible Gastroenteritis Virus Replication
Authors: Guo Longjun , Yu Haidong , Gu Weihong , Luo Xiaolei , Li Ren , Zhang Jian , Xu Yunfei , Yang Lijun , Shen Nan , Feng Li , Wang Yue 
Published: March 2016

AbstractAutophagy is an evolutionarily ancient pathway that has been shown to be important in the innate immune defense against several viruses. However, little is known about the regulatory role of autophagy in transmissible gastroenteritis virus (TGEV) replication. In this study, we found that TGEV infection increased the number of autophagosome-like double- and single-membrane vesicles in the cytoplasm of host cells, a phenomenon that is known to be related to autophagy. In addition, virus replication was required for the increased amount of the autophagosome marker protein LC3-II. Autophagic flux occurred in TGEV-infected cells, suggesting that TGEV infection triggered a complete autophagic response. When autophagy was pharmacologically inhibited by wortmannin or LY294002, TGEV replication increased. The increase in virus yield via autophagy inhibition was further confirmed by the use of siRNA duplexes, through which three proteins required for autophagy were depleted. Furthermore, TGEV replication was inhibited when autophagy was activated by rapamycin. The antiviral response of autophagy was confirmed by using siRNA to reduce the expression of gene p300, which otherwise inhibits autophagy. Together, the results indicate that TGEV infection activates autophagy and that autophagy then inhibits further TGEV replication.
Two-photon excited photoconversion of cyanine-based dyes
Authors: Kwok Sheldon J. J. , Choi Myunghwan , Bhayana Brijesh , Zhang Xueli , Ran Chongzhao , Yun Seok-Hyun 
Published: March 2016

AbstractThe advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits theirin vivoapplicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.
Otolith Microchemistry Reveals Natal Origins of Walleyes in Missouri River Reservoirs
Authors: Carlson Andrew K. , Fincel Mark J. , Graeb Brian D. S. 
Published: March 2016

Abstract
Reproductive habitats are vital for sustaining fish populations, but their location and relative natal contributions are often unknown or poorly understood. We used otolith microchemistry to examine natal origins of Walleyes Sander vitreus in Missouri River reservoirs (i.e., Lake Oahe, Lake Sharpe, Lake Francis Case, and Lewis and Clark Lake) in North Dakota and South Dakota. Water Sr:Ca and Ba:Ca were spatially heterogeneous and temporally consistent in all impoundments. Otolith Sr:Ca and Ba:Ca from age-0 Walleyes permitted the reclassification of fish to known natal habitats (i.e., tributary, embayment, main stem) and individual sites with 87% and 75% accuracy, respectively. Natal contributions were highest in tributaries, particularly those in Lake Oahe, where 32% of all adults and 77% of Lake Oahe adults hatched. Embayments and main-stem environments had high natal contributions (67–78%) in Lakes Sharpe and Francis Case and Lewis and Clark Lake, where tributaries are less abundant. Our research demonstrates the utility of otolith microchemistry for measuring habitat- and site-specific natal contributions and provides further information that can be used in managing Walleyes in Missouri River reservoirs, particularly for broodstock collection, habitat protection and restoration, and harvest regulations.
Received April 17, 2015; accepted December 11, 2015 Published online March 30, 2016
Effects of Different Corn Starch Levels on Growth, Protein Input, and Feed Utilization of Juvenile Hybrid Grouper (male Epinephelus lanceolatus × female E. fuscoguttatus)
Authors: Luo Yuan , Wu Xiaoyi , Li Weifeng , Jiang Shuntian , Lu Senda , Wu Mingjuan 
Published: March 2016

Abstract
An 8-week growth trial was conducted to study the effects of different corn starch levels on growth and feed utilization of juvenile hybrid grouper (male Giant Grouper Epinephelus lanceolatus × female Brown-marbled Grouper E. fuscoguttatus). Five isonitrogenous (48% crude protein, dry weight basis) and isolipidic (10% crude lipid, dry weight basis) diets were formulated to contain 0, 7, 14, 21, or 28% corn starch, respectively. Each dietary treatment had three replicates. Groups of 22 hybrid grouper juveniles (average initial weight, 2.74 g/fish) were stocked into small floating cages (120 cm long × 70 cm wide × 50 cm high). Triplicate groups of fish were fed each dietary treatment twice daily (0800 and 1600 hours) to apparent satiation. Water quality was monitored daily. Weight gain (WG) of hybrid grouper displayed no significant differences among all experimental groups. Fish fed 0% corn starch had significantly higher daily protein intake than fish fed 7, 14, 21, and 28% corn starch. The feed conversion ratio (FCR) and daily feed intake (FI) were significantly higher in fish fed diets with 0% corn starch, but FCR and FI were not observed to vary among fish fed any of the other experimental diets. Fish fed 0% corn starch had a lower protein efficiency ratio (PER) than fish fed other experimental diets, and the differences were significant when compared with fish fed 21% or 28% corn starch. Intraperitoneal fat (IPF) ratios did not show significant variations among all experimental treatments. The hepatosomatic index (HSI) of fish fed 0% corn starch were significantly lower than that of fish fed 14, 21, and 28% corn starch. Whole-body moisture, protein, lipid, and ash contents as well as muscle moisture, protein, and lipid contents showed no significant differences among all experimental groups. Hepatic glycogen content increased as dietary corn starch level increased. Fish fed the diet with no corn starch had more protein content in the liver than fish fed 14, 21, or 28% corn starch. Generally, supplementations of 7–28% dietary corn starch could reduce dietary protein input and improve feed utilization of hybrid grouper without negatively affecting their growth.
Dorsal anterior cingulate and ventromedial prefrontal cortex have inverse roles in both foraging and economic choice
Authors: Shenhav Amitai , Straccia Mark A. , Botvinick Matthew M. , Cohen Jonathan D. 
Published: March 2016

AbstractRecent research has highlighted a distinction between sequential foraging choices and traditional economic choices between simultaneously presented options. This was partly motivated by observations in Kolling et al. (2012) [KBMR] that these choice types are subserved by different circuits, with dorsal anterior cingulate (dACC) preferentially involved in foraging and ventromedial prefrontal cortex (vmPFC) preferentially involved in economic choice. To support this account, KBMR used fMRI to scan human subjects making either a foraging choice (between exploiting a current offer or swapping for potentially better rewards) or an economic choice (between two reward-probability pairs). This study found that dACC better tracked values pertaining to foraging, while vmPFC better tracked values pertaining to economic choice. We recently showed that dACC’s role in these foraging choices is better described by the difficulty of choosing than by foraging value, when correcting for choice biases and testing a sufficiently broad set of foraging values (Shenhav et al., 2014). Here, we extend these findings in three ways. First, we replicate our original finding with a larger sample and a task modified to address remaining methodological gaps between our previous experiments and that of KBMR. Second, we show that dACC activity is best accounted for by choice difficulty alone (rather than in combination with foraging value) during both foraging and economic choices. Third, we show that patterns of vmPFC activity, inverted relative to dACC, also suggest a common function across both choice types. Overall, we conclude that both regions are similarly engaged by foraging-like and economic choice.
Exploring the potential effects of expatriate adjustment direction
Authors: Zhang Ying , Oczkowski Edward 
Published: March 2016

Purpose– The expansion of the phenomenon of two-way flow expatriation due to the accelerated process of globalization has resulted in an increasing need for a better understanding of cross-cultural transitions. Given the absence of convincing a priori theoretical explanations, as part of an inductive discovery process, the purpose of this paper is to examine the relationships between cultural intelligence (CQ), job position, and cross-cultural adjustment (CCA) for expatriates.Design/methodology/approach– Explicit consideration is given to uncovering the potential importance of cultural distance asymmetry (CDA) effects. Structural equation modelling techniques are employed to analyse survey data from a two-flow sample of expatriates between Australia and China.Findings– Results indicate that motivational CQ has a statistically significant effect on CCA. CDA is found to moderate the relationship between job positions and expatriate adjustment, such that the relationship depends on the direction of cultural flow between more and less authoritarian cultural contexts.Originality/value– These findings discover and highlight the potential importance of identifying the direction of cultural flows of expatriation in understanding successful expatriates’ CCA.
Global implications of the indigenous epistemological system from the east
Authors: Li Peter Ping 
Published: March 2016

Purpose– The author argues and explains that the indigenous Eastern epistemological system of Yin-Yang balancing should be taken as a novel system or frame of thinking, which is deeply rooted in the indigenous Eastern culture traditions, but it has significant global implications, especially in the domain of paradox management. The purpose of this paper is twofold: first, to provide a detailed elaboration of the indigenous Eastern epistemological system of Yin-Yang balancing in contrast to the Western logic systems; and second, to provide a roadmap for applying the system of Yin-Yang balancing to complex issues in the area of management, in general, and paradoxical issues, in particular.Design/methodology/approach– This is a conceptual paper with a focus on theory-building.Findings– The author elaborates on the indigenous features of Yin-Yang balancing, in contrast to Aristotle’s formal logic and Hegel’s dialectical logic in the West, to further explore the former’s global implications for the increased attention to research on paradox management. In particular, the author posits that Yin-Yang balancing appears to be better suited for paradox management than the more commonly used logics available in the Western literature. Built upon the Yin-Yang balancing, a practical tool of Duality Map for paradox management is proposed.Research limitations/implications– The system of Yin-Yang balancing proposed in this paper has the potential to embrace logical systems available in the West into a geocentric (East-meeting-West) meta-system. This paper further shows how to apply Yin-Yang balancing with the tool of Duality Map to the most salient paradoxes in the domain of management, including value-profit balance (triple bottom lines), exploration-exploitation balance (ambidexterity), cooperation-competition balance (co-opetition), globalization-localization balance (glocalization), institution-agency balance (institutional entrepreneurship), simultaneously positive and negative attitudes toward an entity (ambivalence), and etic-emic balance (geocentric) across all domains of management research.Originality/value– The primary challenge for management researchers is to find a way to achieve a geocentric integration between the West and the East at the fundamental level of philosophy. The hope is that the philosophical traditions in the East will facilitate such integration. In particular, the Eastern philosophy of wisdom has a unique capacity to reframe paradox from a negative problem (i.e. a problem of inconsistency to be resolved by dualism in terms of separating opposite elements) to a positive solution (i.e. a solution of completeness or holism to be achieved by duality in terms of partially separating and partially integrating opposite elements).
Effects of 8Ps of services marketing on student selection of self-financing sub-degree programmes in Hong Kong
Authors: Lau Melissa May Yee 
Published: March 2016

Purpose
– The purpose of this paper is to investigate how the effects of 8Ps of services marketing affect students’ selection of self-financing sub-degree programmes in Hong Kong. The factors that affect students’ selection of self-financing sub-degree programmes have not been studied in higher education market of Hong Kong. This research is to fill the gap by examining the effects of 8Ps (“Product Elements”, “Price and Other User Outlays”, “Place and Time”, “Promotion and Education”, “People”, “Process”, “Physical Environment” and “Productivity and Quality”) on self-financing sub-degree programmes in Hong Kong.

Design/methodology/approach
– The research taken was a quantitative survey of students at Community College at Lingnan University in Hong Kong.

Findings
– The results reveal that “Productivity and Quality” is the most important element of 8Ps of services marketing. Accreditation of programmes seeking recognition in Hong Kong and overseas can increase student enrolment. “Promotion and Education” element is the least important element of 8Ps of services marketing. Self-financed higher education institutions should develop strategies to build relationships with the secondary school teachers and counsellors rather than invest money on advertising.

Research limitations/implications
– The data were collected from a particular community college in Hong Kong only.

Practical implications
– Management can increase student recruitment by allocating minimum amount of limited resources to recruit maximum number of students.

Originality/value
– This research adds knowledge to the marketing of higher education in Hong Kong. The management of self-financing sub-degree programmes can use the findings of this research as a reference to develop their marketing strategies.
A comparison between flipped and lecture-based instruction in the calculus classroom
Authors: Scott Catherine Elizabeth , Green Linda E , Etheridge Debra Lynn 
Published: March 2016

Purpose– The purpose of this paper is to enhance students’ mastery of course material in a second semester calculus course and assess the effectiveness of a traditional “lecture-based” instructional model compared to a modified “flipped classroom” model using a mixed method research design.Design/methodology/approach– Evaluation of effectiveness was measured by having students in all four courses take the same pre-post exams and participate in a survey to assess their opinion of each instructional model. Undergraduate students covered the same content using two different modes of instruction.Findings– Within a comparable group of students, participants in the flipped course had similar content knowledge gains as the lecture-based course. Based on responses obtained in blinded student surveys, the authors found that the use of an online homework system (WebAssign) and in-class quizzes were critical motivating factors that likely contributed to the increase in student performance. The flipped classroom instruction was more effective at increasing interest in the subject and promoting student discourse around problem solving. Taken together, the findings support the flipped classroom model as an equally effective means in which to disseminate key calculus concepts to undergraduate students.Research limitations/implications– Findings from this study are limited to content knowledge gains in calculus over a 14-week time period, and student attitudes toward the mode of instruction they received. More research is needed to study the depth of learning that occurs in small group problem solving and interactions between faculty-and-student and student-to-student.Originality/value– While many course redesigns focus on incorporating more project-based or laboratory-based learning opportunities, the redesign used in this study was focussed on different ways to deliver the same content. Results from this study suggest that a blended type of learning would be optimal for learning calculus concepts with a strategic use of video content concepts.