bluuebunny/crossref_metadata_2025_split huggingface dataset:
N6-methyladenosine modification and the YTHDF2 reader protein play cell type specific roles in lytic viral gene expression during Kaposi’s sarcoma-associated herpesvirus infection
Authors: Hesser Charles , Karijolich John , Dominissini Dan , He Chuan , Glaunsinger Britt 
Published: October 2017

AbstractMethylation at theN6position of adenosine (m6A) is a highly prevalent and reversible modification within eukaryotic mRNAs that has been linked to many stages of RNA processing and fate. Recent studies suggest that m6A deposition and proteins involved in the m6A pathway play a diverse set of roles in either restricting or modulating the lifecycles of select viruses. Here, we report that m6A levels are significantly increased in cells infected with the oncogenic human DNA virus Kaposi’s sarcoma-associated herpesvirus (KSHV). Transcriptome-wide m6A-sequencing of the KSHV-positive renal carcinoma cell line iSLK.219 during lytic reactivation revealed the presence of m6A across multiple kinetic classes of viral transcripts, and a concomitant decrease in m6A levels across much of the host transcriptome. However, we found that depletion of the m6A machinery had differential pro- and anti-viral impacts on viral gene expression depending on the cell-type analyzed. In iSLK.219 and iSLK.BAC16 cells the pathway functioned in a pro-viral manner, as depletion of the m6A writer METTL3 and the reader YTHDF2 significantly impaired virion production. In iSLK.219 cells the defect was linked to their roles in the post-transcriptional accumulation of the major viral lytic transactivator ORF50, which is m6A modified. In contrast, although the ORF50 mRNA was also m6A modified in KSHV infected B cells, ORF50 protein expression was instead increased upon depletion of METTL3, or, to a lesser extent, YTHDF2. These results highlight that the m6A pathway is centrally involved in regulating KSHV gene expression, and underscore how the outcome of this dynamically regulated modification can vary significantly between cell types.Author SummaryIn addition to its roles in regulating cellular RNA fate, methylation at theN6position of adenosine (m6A) of mRNA has recently emerged as a mechanism for regulating viral infection. While it has been known for over 40 years that the mRNA of nuclear replicating DNA viruses contain m6A, only recently have studies began to examine the distribution of this modification across viral transcripts, as well as characterize its functional impact upon viral lifecycles. Here, we apply m6A-sequencing to map the location of m6A modifications throughout the transcriptome of the oncogenic human DNA virus Kaposi’s sarcoma-associated herpesvirus (KSHV). We show that the m6A machinery functions in a cell type specific manner to either promote or inhibit KSHV gene expression. Thus, the KSHV lifecycle is impacted by the m6A pathway, but the functional outcome may depend on cell lineage specific differences in m6A-based regulation.
An automated barcode tracking system for behavioural studies in birds
Authors: Gustavo’  , Graving Jacob M. , Klarevas-Irby James A. , Maldonado-Chaparro Adriana A. , Mueller Inger , Farine Damien R. 
Published: October 2017

AbstractRecent advances in technology allow researchers to automate the measurement of animal behaviour. These methods have multiple advantages over direct observations and manual data input as they reduce bias related to human perception and fatigue, and deliver more extensive and complete data sets that enhance statistical power. One major challenge that automation can overcome is the observation of many individuals at once, enabling whole-group or whole-population tracking.We provide a detailed description for implementing an automated system for tracking birds. Our system uses printed, machine-readable codes mounted on backpacks. This simple, yet robust, tagging system can be used simultaneously on multiple individuals to provide data on bird identity, position and directionality. Further, because our codes and backpacks are printed on paper, they are very lightweight.We describe the implementation of this automated system on two flocks of zebra finches. We test different camera options, and describe their advantages and disadvantages. We show that our method is reliable, relatively easy to implement and monitor, and with proper handling, has proved to be safe for the birds over long periods of time. Further, we highlight how using single-board computers to control the frequency and duration of image capture makes this system affordable, flexible, and adaptable to a range of study systems.The ability to automate the measurement of individual positions has the potential to significantly increase the power of both observational and experimental studies. The system can capture both detailed interactions (using video recordings) and repeated observations (e.g. once per second for the entire day) of individuals over long timescales (months or potentially years). This approach opens the door to tracking life-long relationships among individuals, while also capturing fine-scale differences in behaviour.
Extracting orthogonal subject- and behavior-specific signatures from fMRI data using whole-brain effective connectivity
Authors: Pallarés Vicente , Insabato Andrea , Sanjuán Ana , Kühn Simone , Mantini Dante , Deco Gustavo , Gilson Matthieu 
Published: October 2017

AbstractThe study of brain communication based on fMRI data is often limited because such measurements are a mixture of session-to-session variability with subject- and condition-related information. Disentangling these contributions is crucial for real-life applications, in particular when only a few recording sessions are available. The present study aims to define a reliable standard for the extraction of multiple signatures from fMRI data, while verifying that they do not mix information about the different modalities. In particular, condition-specific signature should not be contaminated by subject-related information. Practically, signatures correspond to subnetworks of directed interactions between brain regions (typically 100 covering the whole brain) supporting the subject and condition identification for single fMRI sessions. The key for robust prediction is using effective connectivity instead of functional connectivity. Our method demonstrates excellent generalization capabilities for subject identification in two datasets, using only a few sessions per subject as reference. Using another dataset with resting state and movie viewing, we show that the two signatures related to subjects and tasks correspond to distinct subnetworks, which are thus topologically orthogonal. Our results set solid foundations for applications tailored to individual subjects, such as clinical diagnostic.
Asymmetric migration decreases stability but increases resilience in a heterogeneous metacommunity
Authors: Limdi Anurag , Pérez-Escudero Alfonso , Li Aming , Gore Jeff 
Published: October 2017

AbstractMany natural communities are spatially distributed, forming a network of subcommunities linked by migration. Migration patterns are often asymmetric and heterogeneous, with important consequences on the ecology and evolution of the species. Here we investigated experimentally how asymmetric migration and heterogeneous structure affect a simple metacommunity of budding yeast, formed by one strain that produces a public good and a non-producer strain that benefits from it. We find that asymmetric migration increases the fraction of producers in all subpopulations of the metacommunity. Furthermore, asymmetric migration decreases the metacommunity’s tolerance to challenging environments, but increases its resilience to transient perturbations. This apparent paradox occurs because tolerance to a constant challenge depends on the weakest subpopulations of the network, while resilience to a transient perturbation depends on the strongest ones.One Sentence SummaryAsymmetric migration decreases the stability of experimental yeast metacommunities but increases their resilience to transient shocks.
Tonic and transient oscillatory brain activity during acute exercise
Authors: Ciria Luis F. , Luque-Casado Antonio , Sanabria Daniel , Holgado Darias , Ivanov Plamen Ch. , Perakakis Pandelis 
Published: October 2017

AbstractThe physiological changes that occur in the main body systems and organs during physical exercise are well described in the literature. Despite the key role of brain in processing afferent and efferent information from organ systems to coordinate and optimize their functioning, little is known about how the brain works during exercise. The present study investigated tonic and transient oscillatory brain activity during a single bout of aerobic exercise. Twenty young males (19-32 years old) were recruited for two experimental sessions on separate days. Electroencephalographic (EEG) activity was recorded during a session of cycling at 80% (moderate-to-high intensity) of VO2max(maximum aerobic capacity) while performing an oddball task where participants had to detect infrequent targets presented among frequent non-targets. This was compared to a (baseline) light intensity session (30% VO2max). The light intensity session was included to control for any potential effect of dual-tasking (i.e., pedaling and performing the oddball task). A warm-up and cool down periods were completed before and after exercise, respectively. A cluster-based nonparametric permutations test showed an increase in power across the entire frequency spectrum during the moderate-to-high intensity exercise, with respect to light intensity. Further, we found that the more salient target lead to lower increase in (stimulus-evoked) theta power in the 80% VO2maxwith respect to the light intensity condition. On the contrary, higher decrease alpha and lower beta power was found for standard trials in the moderate-to-high exercise condition than in the light exercise condition. The present study unveils, for the first time, a complex brain activity pattern during acute exercise (at 80% of maximum aerobic capacity). These findings might help to elucidate the nature of changes that occur in the brain during physical exertion.
Allocation of responsibility among pork supply chain players
Authors: Wu Linhai , Qiu Guangqian , Lu Jiao , Zhang Minghua , Wen Xiaowei 
Published: October 2017

Purpose
The purpose of this paper is to investigate the responsibility that should be taken by different pork supply chain participants to ensure pork quality and safety, with the aim of providing some guidance for strengthening the supervision of pork quality and safety.

Design/methodology/approach
The pig farmer survey and the pork consumer survey were conducted in Funing County, Jiangsu Province, using the best-worst scaling (BWS) and a mixed logit model.

Findings
The results showed that the designation of responsibility for ensuring pork quality and safety was of, in descending order, feed producers and suppliers, backyard farmers and farms of designated size, pork processing workshops and companies of and above designated size, slaughterhouses, supermarkets, farmer’s markets, pig transporters, and consumers. Both pig farmers and pork consumers believed that those involved in the initial pork supply chain should take greater responsibility for pork quality and safety.

Originality/value
Allocation of responsibilities across the entire pork industry chain was investigated from the perspective of pig farmers and pork consumers using the BWS and a mixed logit model. The results of this study might explain the unique problems that occur in pork supply chain management in large developing countries like China.
Food professionals’ opinions of the Food Studies curriculum in Australia
Authors: Nanayakkara Janandani , Margerison Claire , Worsley Anthony 
Published: October 2017

Purpose
The purpose of this paper is to investigate the food system professionals’ opinions of a new senior secondary school food literacy curriculum named Victorian Certificate of Education Food Studies in Victoria, Australia.

Design/methodology/approach
A purposive sample of 34 food system professionals from different sub-sectors within the Australian food system was interviewed individually in late 2015 and early 2016. Interviews were analysed using the template analysis technique.

Findings
Most participants appreciated the extensive coverage of food literacy aspects in this new curriculum. However, many suggested amendments to the curriculum including pay less emphasis on food history-related topics and pay more focus on primary food production, nutrition awareness and promotion, and food security, food sovereignty, social justice, and food politics.

Practical implications
A well-structured, comprehensive secondary school food literacy curriculum could play a crucial role in providing food literacy education for adolescents. This will help them to establish healthy food patterns and become responsible food citizens. The findings of this study can be used to modify the new curriculum to make it a more comprehensive, logical, and feasible curriculum. Moreover, these findings could be used to inform the design of new secondary school food literacy curricula in Australia and other countries.

Originality/value
The exploration of perspectives of professionals from a broad range of food- and nutrition-related areas about school food literacy education makes this study unique. This study highlights the importance of food professionals’ opinions in secondary school food-related curricula development.
Quality parameters of freeze-dried peach snack
Authors: Pieniazek Facundo , Messina Valeria 
Published: October 2017

Purpose
The causes of food waste or loss are numerous and occur at the stages of production, processing, retailing and consumption. Peaches must be processed quickly to maintain their quality because they are seasonal and their shelf life is short. The purpose of this paper is to evaluate the effect of freeze drying on quality parameters of stored peaches in order to increase shelf life, consumption and to decrease waste of fruit.

Design/methodology/approach
Samples were ripped on maturity time, stored for 16 days and sampled out and freeze-dried at every four-day interval. Microstructure was carried out by applying scanning electron microscopy, texture and colour parameters by image analysis techniques and physicochemical parameters by conventional analysis.

Findings
Statistical differences (p<0.05) were obtained of colour and texture (stored and freeze-dried rehydrated samples) and for water activity and porosity (freeze-dried samples). Results revealed that after 12 days of storage/freeze, dried peach snacks showed lower pore size with higher amounts of pore, which affected rehydration process leading to a harder sample. Colour was also affected leading to a darkener fruit.

Originality/value
This research incorporates a quick approach to quality applying image analysis techniques and it will benefit to increase shelf life and consumption of fat-free snack peach and to decrease waste of fruit.
A profile of older green tea consumers in the USA
Authors: Berhaupt-Glickstein Amanda , Hallman William 
Published: October 2017

Purpose
The purpose of this paper is to identify the demographic and psychographic characteristics of older green tea consumers in the USA. By understanding this segment’s background, perceptions, and behaviors, health and marketing professionals can tailor messages to reach clients and consumers.

Design/methodology/approach
An online survey was completed in January 2014 with 1,335 older adult consumers (=55 years old). Data were analyzed using descriptive statistics and binomial logistic regression.

Findings
More than half (n=682, 51.2 percent) of respondents drank green tea. Most green tea consumers in this sample are college-educated and employed female home owners. The odds for green tea consumption are greater if a respondent is in good health, was informed about diet and health, or made a health-related dietary change in the past year. There are greater odds of consumption if the respondent is familiar with the relationship between drinking green tea and the reduced risk of cancer however, the importance of health statements on product labels are not predictive of consumption.

Research limitations/implications
This study was conducted in the USA and with older adults. Future research should explore characteristics of younger consumers, i.e. 18-54 years old.

Practical implications
Health educators, regulators, and marketing professionals may use this profile to tailor messages that speak to consumers and client’s values and motivations.

Originality/value
To the authors’ knowledge, this is the first profile of older adult green tea consumers in the USA.
What is the pattern of self-harm and prison rule-breaking behaviour in personality disordered offenders in a high secure prison?
Authors: de Motte Claire , Bailey Di , Hunter Melanie , Bennett Alice L. 
Published: October 2017

Purpose
The purpose of this paper is to describe the pattern of self-harm (SH) and proven prison rule-breaking (PRB) behaviour in prisoners receiving treatment for personality disorders (PDs) within a high security prison.

Design/methodology/approach
A comparative quantitative case study design supported the understanding of the frequency and pattern of SH and PRB behaviour across two stages of a PD treatment programme for 74 male prisoners. Data obtained from the prison’s records were analysed using dependent t-tests, χ2 test of independence and time-frequency analyses.

Findings
Inferential statistics showed that the frequency of SH and PRB behaviour statistically increased across two phases of the PD treatment programme; however, the method of SH or type of PRB behaviour engaged in did not change. Mapping the frequencies of incidents using a time-frequency analysis shows the patterns of both behaviours to be erratic, peaking in the latter phase of treatment, yet the frequency of incidents tended to decline over time.

Originality/value
This is the first study to explore SH and PRB behaviours in men across two phases of a PD treatment programme. This study highlights the need for continued psychological support alongside the PD treatment programme with a focus on supporting men in treatment to effectively manage their SH and PRB behaviour.
Researchers’ attitudes towards the use of social networking sites
Authors: Greifeneder Elke , Pontis Sheila , Blandford Ann , Attalla Hesham , Neal David , Schlebbe Kirsten 
Published: October 2017

Purpose
The purpose of this paper is to better understand why many researchers do not have a profile on social networking sites (SNS), and whether this is the result of conscious decisions.

Design/methodology/approach
Thematic analysis was conducted on a large qualitative data set from researchers across three levels of seniority, four countries and four disciplines to explore their attitudes toward and experiences with SNS.

Findings
The study found much greater scepticism toward adopting SNS than previously reported. Reasons behind researchers’ scepticism range from SNS being unimportant for their work to not belonging to their culture or habits. Some even felt that a profile presented people negatively and might harm their career. These concerns were mostly expressed by junior and midlevel researchers, showing that the largest opponents to SNS may unexpectedly be younger researchers.

Research limitations/implications
A limitation of this study was that the authors did not conduct the interviews, and therefore reframing or adding questions to specifically unpack comments related to attitudes, feelings or the use of SNS in academia was not possible.

Originality/value
By studying implicit attitudes and experiences, this study shows that instead of being ignorant of SNS profiles, some researchers actively opt for a non-use of profiles on SNS.
Multilayer document model for semantic document management services
Authors: Sladić Goran , Cverdelj-Fogaraši Igor , Gostojić Stevan , Savić Goran , Segedinac Milan , Zarić Miroslav 
Published: October 2017

PurposeThe purpose of this paper is to identify the benefits of an approach in which document management systems (DMSs) are based on a formal and explicit document model, primarily in terms of facilitating domain-specific customization.Design/methodology/approachWithin this paper, a generic document model is proposed. The model consists of two layers. A general purpose layer, which represents common features of the documents, and a domain-specific layer, modeling properties particular to application domain. The general purpose layer is based on ISO 82045, providing high degree of interoperability with other systems developed with respect to this set of standard.FindingsSplitting document model into the layers enables DMSs to be tailored for each particular domain of application, depending on the general purpose layer. The existence of domain-specific layer allows documents to be interpreted differently in different domains of application.Practical implicationsIn order to enable customization of DMS for a particular domain, the implementation of domain-specific document layer is required. Also, the proposed model does not explicitly deal with document dynamics.Originality/valueThe proposed document ontology is general enough to provide the representation of documents not depending on a specific scope of application, yet flexible enough to enable extensions through which domain-specific document features can be expressed. The separation of document model enables development of core DMS offering services relying explicitly on the general purpose layer on one hand, as well as domain-specific customization of DMS on the other.
Exploring task-service fit and usefulness on branded applications continuance
Authors: Fang Yu-Hui 
Published: October 2017

Purpose
Providing that branded applications (apps) became a new trend in mobile marketing, the purpose of this study, thus, is to explore how to promote app users’ continuance intention and purchase intention (i.e. “app continuance”) toward a specific branded app.

Design/methodology/approach
By integrating both goods-dominant logic (GDL) and service-dominant logic (SDL), this study uses a unifying model to examine whether perceived usefulness and task-service fit (TSF) have different effects on the two parts of app continuance. This study identifies task characteristic and four service characteristics (interactivity, presence, localization and ubiquity) as antecedents of TSF. Furthermore, psychological barriers are examined as mediators of TSF and purchase intention within SDL. Data collected from 631 users of the targeted branded apps support all of the proposed hypotheses.

Findings
The findings show that besides perceived usefulness, TSF is an essential determinant of both app continuance in the context of branded apps and a partial mediator of psychological barriers between TSF and purchase intention.

Originality/value
Unlike prior studies, which have focused on traditional GDL to examine continuance intention, this study incorporates SDL and the notion of psychological barriers to explore such matters. The evidence concerning the significantly higher explanatory power of the full model suggests that a deeper understanding of the antecedents of app continuance is possible when the alternative view is taken into consideration, thus providing a promising avenue for future research.