bluuebunny/crossref_metadata_2025_split huggingface dataset:
Israeli neonatal herpes simplex infection: Unique epidemiology and clinical profile
Authors: Lagziel Tal Golan , Jurkowicz Menucha , Gordon Oren , Mor Merav , Megged Orli , Nasrallah Elias , Jacob Ron , Melamed Rimma , Blustein Shani Levin , Tasher Diana , Guri Alex , Regev Asaf , Linder Ilan , Brosh‐Nissimov Tal , Bamberger Ellen , Lavy Ronni Gur , Lipman‐Arens Shelly , Shehadeh Shereen , Farah Hanna , Stein Michal 
Published: September 2024

AbstractNeonatal herpes simplex virus (NHSV) infection, although rare, is related to high rates of morbidity and mortality, both can be reduced by prompt acyclovir treatment. In contrast, enterovirus is a common cause of neonatal meningitis, with relatively negligible incidence of serious illness or long‐term sequelae, and its clinical course is not affected by acyclovir treatment. Acyclovir is associated predominantly with renal adverse effects, and its widespread application in the context of neonatal fever is not always justified. To further characterized the Israeli NHSV infection epidemiology and to evaluate the distinct clinical characteristics of NHSV and neonatal enteroviral meningitis (NEM), Israeli NHSV patients, hospitalized between January 2015 and April 2022 in 22 medical centers were assessed, together with NEM patients, hospitalized at Sheba Medical Center during the same period. NHSV demographic and clinical characteristics were documented and compared to those of NEM. Eighty‐five NHSV (73% males) and 130 NEM (62% males) patients were included. The incidence of NHSV was 5.9/100 000 live births, the common phenotype and HSV type were SEM (53%) and HSV1 (91%), respectively. Horizontal transmission was suspected in 50% cases (of which 67% underwent a Jewish ritual circumcision with direct wound sucking, 33% had relatives with highly suspicious herpetic lesions). Compared with NEM, NHSV tends to present with rash (14% vs. 60%, p < 0.01) and seizures (0% vs. 6%, p = 0.02), while fever, irritability and poor feeding appear more frequently in NEM (94% vs. 18%, p < 0.01; 37% vs. 1%, p < 0.01; 25% vs. 1%, p < 0.01, respectively). Of NEM patients, 28% were treated with acyclovir. Our results mark a decrease in the incidence rate of NHSV in Israel and a prominent mode of horizontal infection acquisition. We underscore the unique localized phenotype of NHSV, in contrast to enterovirus, which tends to cause a systemic disease with constitutional symptoms. These findings should be considered when evaluating the need for comprehensive empirical treatment for HSV in the context of neonatal fever, or according to a certain clinical presentation.
Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01
Authors: Liu Hui , He Hailin , Wei Lulu , Lei Yinfeng , Liu Mengyu , Ding Yifei , Su Xiaochun , Wang Jinxuan , Yang Jian , Li Shubo 
Published: September 2024

AbstractBACKGROUNDAs a novel type of extracellular polysaccharide produced by Sphingomonas sp., welan gum has been widely applied in various fields because of its excellent properties. The study has improved the fermentation process.ResultsThe initial sucrose concentration, temperature and stirring speed were set to 20 g L−1, 33 °C and 400 rpm, respectively, and 13.3 g L−1 sucrose was added at 24, 40 and 56 h. The temperature and stirring speed were then set at 28 °C and 600 rpm from 24 to 48 h and 28 °C and 600 rpm from 48 to 72 h, respectively. As a result, welan gum production, dry cell weight, sucrose conversion rate and viscosity were correspondingly increased to 38.60 g L−1, 5.47 g L−1, 0.64 g g−1 and 3779 mPa·s, respectively. In addition, the mechanism by which fermentation strategy promotes welan gum synthesis was investigated by transcriptome analysis.CONCLUSIONImproving respiration and ATP supply, reducing unnecessary protein synthesis, and alleviating competition between cell growth and welan gum synthesis contribute to promoting the fermentation performance of Sphingomonas sp., thus providing a practical strategy for efficient welan gum production. © 2024 Society of Chemical Industry.
How Does the Green and Non‐Green Star Moderate the Effect of Hotel Environmental Strategy on Sustainable Awareness and Green Employee Behavior?
Authors: Yayla Ozgur , Keles Huseyin , Silik Cemal Ersin , Akbulut Cemile 
Published: September 2024

ABSTRACTThe objective of this research is to determine the effects of hotel environmental strategies on sustainable awareness perception and green employee behavior in the hospitality industry. The moderator role of the green star practices in the relationships between the variables has also been searched. The study data have been obtained from the employees of five‐star hotels in Antalya, Türkiye. SPSS package program and Smart PLS were preferred in examining the relationships. As a result, significant relationships have been determined, and the green star practices moderate the relationship between them. Besides, the moderator role of green star practices has been analyzed, and this is the main difference of this research from the other hospitality studies in the literature. In the light of the research results, it can be stated that hotel business managers should empower their employees on green behaviors by increasing their motivation for a sustainable hospitality sector.
Medical Tourism Attribute Competitiveness: An Importance–Performance Analysis
Authors: Jalilian Habib , Khodayari‐Zarnaq Rahim , Rahmani Hojjat , Torkzadeh Leila 
Published: September 2024

ABSTRACTThis study aimed to analyze the performance of medical tourism in Iran using importance–performance analysis (IPA). In this cross‐sectional study, five hundred ninety‐six medical tourists from West Asian countries (Iraq, Azerbaijan, Kuwait, Qatar, Bahrain, Oman, Syria, Lebanon, Oman, Saudi Arabia, and Yemen) referring to health centers to receive medical services in Iran in 2020 were recruited. A standard IPA questionnaire was used for data collection. IP gaps were negative in all three attributes (medical treatments and service attributes, destination attributes, and tourism‐specific attributes). Respondents attached the highest importance to medical treatments and service attributes. The highest IP gap was related to medical treatments and service attributes. The results highlighted that the Iranian tourism industry is not performing well in many areas. Tourism managers and stakeholders should adopt useful strategies for improving those areas with high importance and low performance.
Assessing Clinical Variables Associated With Femoral Muscle Decay as Measured by Point‐of‐Care Ultrasound in Critically Ill Children
Authors: Kim Esther J. , Wai Kitman , Pedoeim Leah , Basu Sonali 
Published: September 2024

ObjectivesSkeletal muscle wasting is a common occurrence in critical illness, often resulting in intensive care unit (ICU)‐acquired weakness. This study aims to identify clinical factors associated with muscle decay in mechanically ventilated critically ill children. Utilizing point‐of‐care ultrasound, a noninvasive and cost‐effective tool, we assess muscle decay through ultrasound of the quadriceps femoris.MethodsA prospective observational study was conducted in a single‐center quaternary‐care pediatric intensive care unit at a children's hospital. A convenience sample of 103 sedated and mechanically ventilated patients were enrolled in this study. Ultrasound measurements of quadriceps femoris muscle thickness were taken, and daily muscle decay rates were calculated. Demographic, clinical, and outcome data were analyzed for correlations with muscle decay.ResultsAmong the enrolled patients, 67 had repeat measurements. Muscle thickness change aligned with prior studies, with a mean daily change of −1.9% [IQR −0.8, −5.0]. Adequate cumulative caloric intake (>60% of goal) correlated with less muscle decay compared with inadequate intake (−1.8 vs −2.4%, P < .001). Average daily muscle change correlated with both ICU and hospital length of stay (LOS) (r = .328, P = .007 and r = .393, P = .001). No significant correlations emerged between muscle change and mortality, disease severity, fluid balance, early mobilization, steroid exposure, or sedative and paralytic use.ConclusionThis study demonstrates early and frequent muscle decay in critically ill children, as detected by point‐of‐care ultrasound. Average daily muscle decay was associated with longer ICU and hospital LOS. Adequate cumulative caloric intake is linked to reduced muscle decay. These findings contribute to understanding muscle wasting in critically ill pediatric patients.
High accuracy in lower limb alignment analysis using convolutional neural networks, with improvements needed for joint‐level metrics
Authors: Hoffmann Christof , Göksu Fatih , Klöpfer‐Krämer Isabella , Watrinet Julius , Blum Philipp , Hungerer Sven , Schröter Steffen , Stuby Fabian , Augat Peter , Fürmetz Julian 
Published: September 2024

AbstractObjectiveEvaluation of long‐leg standing radiographs (LSR) is a standardised procedure for analysis of primary or secondary deformities of the lower limbs. Deep‐learning convolutional neural networks (CNN) offer the potential to enhance radiological measurement by increasing reproducibility and accuracy. This study aims to evaluate the measurement accuracy of an automated CNN‐based planning tool (mediCAD® 7.0; mediCAD Hectec GmbH) of lower limb deformities.MethodsIn a retrospective single‐centre study, 164 pre‐ and postoperative bilateral LSRs with uni‐ or bilateral posttraumatic knee arthritis undergoing total knee arthroplasty (TKA) were enroled. Alignment parameters relevant to knee arthroplasty and deformity correction were analysed independently by two observers and a CNN. The intraclass correlation coefficient (ICC) was used to evaluate the accuracy between observers and the CNN, which was further evaluated using absolute deviations, limits of agreement (LoA) and root mean square error (RMSE).ResultsCNN evaluation demonstrated high consistency in measuring leg length (ICC > 0.99) and overall lower limb alignment measures of mechanical tibio‐femoral angle (mTFA) (ICC > 0.97; RMSE < 1.1°). The mean absolute difference between angular measurements were low for overall lower limb alignment (mTFA 0.49–0.61°) and high for specific joint angles (aMPFA 3.86–4.50°). Accuracy at specific joint angles like the mechanical proximal tibial angle (MPTA) and the mechanical lateral distal femur angle (mLDFA) varied between lower limbs with deformity, with and without TKA with greatest difference for TKA (ICC 0.22–0.85; RMSE 1.72–3.65°).ConclusionExcellent accuracy was observed between manual and automated measurements for overall alignment and leg length, but joint‐level metrics need further improvement especially in case of TKA similar to other existing algorithms. Despite the observed deviations, the time‐efficient nature of the algorithm improves the efficiency of the preoperative planning process.Level of EvidenceIV
Significance of Incidental Thyroid Findings in a Large Community‐based Lung Cancer Screening Cohort
Authors: Bertoni Dylan , Schlegel Lauren , Gillmore Kathleen , Brill‐Edwards Madeleine , Armache Maria , Cottrill Elizabeth 
Published: September 2024

ObjectivesInvestigate incidental findings of neck pathology on lung cancer screening computer tomography scans and determine clinical relevance in a population of heavy smokers.MethodsA retrospective chart review was conducted utilizing a database of patients who underwent a chest computed tomography (CT) through a robust community‐based hospital lung cancer screening program in 2019 and 2020. Demographics, medical history, imaging reports, and any pathology results were analyzed.ResultsA total of 1738 patients received a lung screening scan during the study period. The median age of the patient population was 64 (range: 50–79), 886 (51%) were male, and 1599 (92%) were Caucasian. Ninety‐four (5.4%) were found to have neck findings on their CT report, including thyroid nodules, calcifications, heterogeneity or enlargements, pathologic appearing lymph nodes, or parathyroids. Forty‐two patients had evidence of follow‐up for their thyroid findings, and three of these ultimately underwent surgical intervention.ConclusionThe results of our study demonstrate that lung cancer screening chest scans can discover clinically relevant findings in the neck. The risks and benefits of screening needs to be thoroughly evaluated. Given the relatively low incidence of findings in this population, a prospective study in conjunction with screening questionnaire for symptoms may increase the yield of incidental findings.Level of Evidence3 Laryngoscope, 135:964–968, 2025
Oat/Soybean Intercropping Reshape the Soil Bacterial Community for Enhanced Nutrient Cycling
Authors: Ma Huaiying , Zhou Jie , Ge Junyong , Zamanian Kazem , Wang Xingyu , Yang Yadong , Zeng Zhaohai , Zhao Baoping , Hu Yuegao , Zang Huadong 
Published: September 2024

ABSTRACTIntercropping, particularly within legume‐based systems, has been shown to enhance yields and optimize resource use efficiency. Yet, the potential contribution of intercropping on soil microbial communities and functions to soil nutrients cycling are not fully understood. We conducted the same field experiments at Youyu (Site1) and Zhangbei (Site2) in Northern China to evaluate the impact of oat/soybean intercropping on the rhizosphere soil bacterial community structure, composition, and co‐occurrence networks. Our results indicated that intercropping significantly modified the bacterial community structure for both oat and soybean at Site1, with changes observed only in the oat community at Site2. Specifically, intercropping led to a substantial increase in the relative abundance of Bacteroidetes and Patescibacteria in the oat rhizosphere by 48.3% and 65.4% (Site1), respectively. Conversely, in the soybean rhizosphere at Site1, there was a notable decrease in the abundance of Patescibacteria and Nitrospirae by 32.4% and 40.0%, respectively. The soil bacterial functional groups demonstrated robust positive correlations with key soil parameters such as available nitrogen (Nmin), available phosphorus (Avail‐P), and the activities of nitrogen‐ and phosphorus‐acquiring enzymes in the rhizosphere. In conclusion, intercropping is an effective agricultural practice for enhancing nitrogen and phosphorus cycling by reshaping the soil bacterial community, offering a distinct advantage over monoculture practices. This insight underscores the potential of intercropping to foster sustainable soil nutrient management, highlighting the importance of integrating such practices into modern agricultural strategies to ensure long‐term productivity and environmental sustainability.
Tunable Spectral Emission from 0D Rb3ZnBr5 Crystals for Single‐Component Multi‐Color LED Applications
Authors: Chen Xi , Lu Zehan , Pan Yuexiao , Ding Yihong , Lian Hongzhou , Lin Jun , Li Liyi 
Published: September 2024

AbstractThe development of efficient, stable, and cost‐effective luminescent materials is crucial for advancing lighting and display technologies. In this study, a series of tunable luminescent materials based on the novel 0D all‐inorganic perovskite (AIP) crystal Rb₃ZnBr₅ (RZB) is obtained. The emission of Sn2⁺ doped RZB spans the entire visible light range and extends into the near‐infrared (NIR) region, achieving a photoluminescence quantum yield (PLQY) of 75%. The distinct lifetimes combined with theoretical calculations, indicate that the broadband dual‐peak emissions at 496 and 636 nm originate from the singlet and triplet states of Sn2⁺, respectively, which is rarely observed in Sn2⁺‐doped perovskite crystals. The phosphor RZB:Sn2⁺ demonstrates the high thermal stability, along with excellent moisture and air stability. Co‐doping RZB with Sn2⁺ and Mn2⁺ broadens the excitation spectrum and allows precise control over the excitation wavelength, facilitating dynamic transitions from warm white to yellow and green light. This feature is particularly beneficial for multicolor LEDs and multi‐level anti‐counterfeiting applications.
Use of Optical Coherence Tomography to Assess Properties of Cutaneous Defects Following Radiofrequency Microneedling and Laser Treatment
Authors: Seiger Kira , Driscoll William , Messele Feben , Golbari Nicole M. , Fan Xiying , Holmes Jon , Zachary Christopher B. 
Published: September 2024

ABSTRACTObjectivesTo characterize the properties of cutaneous defects created by energy‐based devices using optical coherence tomography.Materials and MethodsRadiofrequency (RF) microneedling and non‐ablative fractional laser (NAFL) treatment were performed in vivo with various parameters. Following treatment, optical coherence tomography (OCT) was used to image and measure cutaneous defects at multiple time points over a 24 h period.ResultsChannel‐like cutaneous defects were visible with OCT following bipolar RF microneedling and NAFL treatment. Using a double pulse technique with RF microneedling yielded a greater number of defects visible with OCT, as well as defects that were deeper and more durable over time. Following treatment with 1927 nm thulium fiber laser, the average diameter of the defects was greater when the energy level was 20 mJ as compared to 10 mJ (0.33 mm vs. 0.27 mm, p < 0.01).ConclusionsCutaneous defects were observed following both RF microneedling and NAFL treatment. Properties of the cutaneous defects varied based on device, treatment setting, and technique, which may be useful in guiding further study of device‐assisted drug delivery.
Zwitterionic Amino‐Acid‐Derived Polyacrylamides with a Betaine Twist – Synthesis and Characterization
Authors: De Breuck Jonas , Jérôme Valérie , Freitag Ruth , Leiske Meike N. 
Published: September 2024

AbstractAmino‐acid‐derived polyzwitterions and polybetaines (PBs) are two promising alternatives to non‐ionic polymers, for example, to increase tumor permeability. In this study, amino‐acid‐derived polyzwitterions are synthesized and a strategy to quarternize the amine in the side chain functional group is developed to combine the advantages of both types. The functional monomer is polymerized via reversible addition−fragmentation chain‐transfer polymerization for which a kinetic study is performed. Further, the impact of the permanent positive charge on amino‐acid‐derived polyzwitterions is studied based on two zwitterionic polymers obtained via post‐polymerization modification (PPM) of Poly(N‐acryloxysuccinimide) to allow good comparison between methylated and non‐methylated polymers. Circular dichroism shows that the stereocenter remains intact during PPM. pH titration and ζ‐potential measurements show that the methylated polymer has a negative ζ‐potential over the measured pH range and, therefore, the polymer remains zwitterionic over a broader pH range than its non‐methylated equivalent. Both polymers are well tolerated by mammalian cells up to concentrations of 1 mg mL−1. The study introduces a path to a new polymer class that combines the advantages of both PBs and amino‐acid‐derived polyzwitterions and highlights the impact a permanent charge has on the physiochemical properties.
The effects of stress on gut virome: Implications on infectious disease and systemic disorders
Authors: Talarico Francesca , Tilocca Bruno , Spagnuolo Rocco , Abenavoli Ludovico , Luzza Francesco , Roncada Paola 
Published: September 2024

AbstractThe role of gut microbiota in health and disease is being thoroughly examined in various contexts, with a specific focus on the bacterial fraction due to its significant abundance. However, despite their lower abundance, viruses within the gut microbiota are gaining recognition for their crucial role in shaping the structure and function of the intestinal microbiota, with significant effects on the host as a whole, particularly the immune system. Similarly, environmental factors such as stress are key in modulating the host immune system, which in turn influences the composition of the gut virome and neurological functions through the bidirectional communication of the gut–brain axis. In this context, alterations in the host immune system due to stress and/or dysbiosis of the gut virome are critical factors in the development of both infectious and noninfectious diseases. The molecular mechanisms and correlation patterns between microbial species are not yet fully understood. This literature review seeks to explore the interconnected relationship between stress and the gut virome, with a focus on how this interaction is influenced by the host's immune system. We also discuss how disturbances in this finely balanced system can lead to the onset and/or progression of diseases.
The cutting‐edge progress in bioprinting for biomedicine: principles, applications, and future perspectives
Authors: Liu Shuge , Chen Yating , Wang Zhiyao , Liu Minggao , Zhao Yundi , Tan Yushuo , Qu Zhan , Du Liping , Wu Chunsheng 
Published: September 2024

AbstractBioprinting is a highly promising application area of additive manufacturing technology that has been widely used in various fields, including tissue engineering, drug screening, organ regeneration, and biosensing. Its primary goal is to produce biomedical products such as artificial implant scaffolds, tissues and organs, and medical assistive devices through software‐layered discrete and numerical control molding. Despite its immense potential, bioprinting technology still faces several challenges. It requires concerted efforts from researchers, engineers, regulatory bodies, and industry stakeholders are principal to overcome these challenges and unlock the full potential of bioprinting. This review systematically discusses bioprinting principles, applications, and future perspectives while also providing a topical overview of research progress in bioprinting over the past two decades. The most recent advancements in bioprinting are comprehensively reviewed here. First, printing techniques and methods are summarized along with advancements related to bioinks and supporting structures. Second, interesting and representative cases regarding the applications of bioprinting in tissue engineering, drug screening, organ regeneration, and biosensing are introduced in detail. Finally, the remaining challenges and suggestions for future directions of bioprinting technology are proposed and discussed.Bioprinting is one of the most promising application areas of additive manufacturing technology that has been widely used in various fields. It aims to produce biomedical products such as artificial implant scaffolds, tissues and organs, and medical assistive devices. This review systematically discusses bioprinting principles, applications, and future perspectives, which provides a topical description of the research progress of bioprinting.