bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative study of the influence of hawthorn (Crataegus monogyna) berry ethanolic extract and butylated hydroxylanisole (BHA) on lipid peroxidation, myoglobin oxidation, consistency and firmness of minced pork during refrigeration
Authors: Papuc Camelia , Predescu Corina Nicoleta , Tudoreanu Liliana , Nicorescu Valentin , Gâjâilă Iuliana 
Published: August 2017

AbstractBACKGROUNDFollowing public concern on the use of synthetic food antioxidants, there is an increasing demand for the application of mixed or purified natural antioxidants to maintain quality of meat products quality during storage. The aim of this research was to investigate the effect of ethanolic extract of hawthorn berry, compared to butylated hydroxylanisole (BHA), on lipid peroxidation, myoglobin oxidation, protein electrophoresis pattern, consistency and firmness of minced pork during refrigeration at 4 °C, and to identify the relationship between chemical modifications and consistency variation.RESULTSAfter 6 days of refrigeration it was found that the thiobarbituric acid reactive substances value of minced pork containing 200 mg GAE kg−1 total phenolics in minced meat (200 HP) was significantly lower (0.1543 ± 0.006 mg) compared to BHA‐treated meat. The ratio of oxymyoglobin to metmyoglobin in treated minced pork was respectively 0.845 for 200 HP and 0.473 for BHA‐treated minced meat. Concentrations of 100 HP or 300 HP will generate statistically higher firmness than BHA in minced pork.CONCLUSIONHawthorn berry ethanolic extract was more effective than BHA in reducing lipid oxidation and protein degradation, for maintaining firmness and consistency of minced pork during 6 days of refrigeration at 4 °C. © 2017 Society of Chemical Industry
Sulfonated poly(styrene‐divinylbenzene) modified with amines and the application for pipette‐tip solid‐phase extraction of carbendazim in apples
Authors: Ma Yuxin , Liu Lingling , Tang Weiyang , Zhu Tao 
Published: August 2017

AbstractSulfonated poly(styrene‐divinylbenzene) modified with five kinds of amine functional groups was applied to the determination of carbendazim in apple samples with a pipette‐tip solid‐phase extraction method. The structures of the polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. Five different modifications of the solid‐phase extraction sorbent based on sulfonated poly(styrene‐divinylbenzene) were tested under static and pipette‐tip solid‐phase extraction conditions. The polymer modified with p‐methoxyaniline showed the best recognition capacity and adsorption amount for carbendazim. Under the optimum conditions, 3.00 mg of the adsorbent, 1.00 mL of ethyl acetate as washing solvent, and 1.00 mL of ammonia/acetonitrile (5:95, v/v) as elution solvent were used in the pretreatment procedure of apple samples. The calibration graphs of carbendazim in methanol were linear over 5.00–200.00 μg/mL, and the limits of detection and quantification were 0.01 and 0.03 μg/mL, respectively. The method recoveries of carbendazim were in the range of 91.31–98.13% with associated intraday relative standard deviations of 0.76–2.13% and interday relative standard deviations of 1.10–1.85%. Sulfonated poly(styrene‐divinylbenzene) modified with p‐methoxyaniline showed satisfactory results (recovery: 97.96%) and potential for the rapid purification of carbendazim in apple samples combined with the pipette‐tip solid‐phase extraction.
Sirolimus as treatment for 19 patients with refractory cervicofacial lymphatic malformation
Authors: Strychowsky Julie E. , Rahbar Reza , O'Hare Meghan J. , Irace Alexandria L. , Padua Horacio , Trenor Cameron C. 
Published: August 2017

ObjectiveLymphatic malformations (LMs) are challenging to manage, particularly those involving the cervicofacial region and airway. Traditional therapy is sclerotherapy and/or resection. We aim to establish the emerging therapeutic role of sirolimus.Study DesignInstitutional review board‐approved retrospective review.MethodsAll patients treated for cervicofacial LM with sirolimus at Boston Children's Hospital, Massachusetts, from November 2012 to October 2016 were included. Chart review included response to therapy (defined as reduction in LM bulk by clinical photographs and radiologic imaging), type of LM (microcystic, macrocystic, mixed), extent of disease, duration of therapy, patient/parent‐reported quality‐of‐life, airway status (tracheostomy dependence), and complications (opportunistic infection, hemorrhage, other). Follow‐up and clinical outcomes were included up until October 2016.ResultsNineteen patients were treated with sirolimus for cervicofacial LM from November 2012 to October 2016 at Boston Children's Hospital. Seven patients remain on uninterrupted sirolimus. Of 12 patients who stopped therapy, seven have resumed due to recurrence of symptoms. All patients demonstrated reduction in LM bulk, ranging from modest to significant. All patients with mucosal vesicles (n = 14) resolved or improved on sirolimus. Six patients developed cellulitis, and four had bleeding within the LM during treatment. No patients developed opportunistic or systemic bacterial infection.ConclusionThe use of sirolimus in the management of cervicofacial LM often is efficacious, with limited adverse events. Long‐term follow‐up, durability of response, and coordination of sirolimus prior to procedural therapies need further study.Level of Evidence4. Laryngoscope, 128:269–276, 2018
Epidermal growth factor receptor detection in serum and saliva as a diagnostic and prognostic tool in oral cancer
Authors: Zanotti Laura , Paderno Alberto , Piazza Cesare , Pagan Eleonora , Bignotti Eliana , Romani Chiara , Bandiera Elisabetta , Calza Stefano , Del Bon Francesca , Nicolai Piero , Ravaggi Antonella 
Published: August 2017

Objectives/HypothesisEpidermal growth factor receptor (EGFR) is a type I transmembrane glycoprotein that is overexpressed in a wide variety of malignancies, including oral squamous cell carcinoma (OSCC). Our objective was to assess the EGFR diagnostic and prognostic value in OSCC by investigating its expression in serum and saliva of patients in comparison with healthy subjects.Study DesignProspective case‐control study.MethodsSerum and saliva samples were collected from a cohort of 63 treatment‐naïve OSCC patients before surgery and a matched group of 60 healthy subjects. EGFR concentrations in serum and saliva were quantified by an enzyme‐linked immunosorbent assay.ResultsOSCC patients showed lower values of serum EGFR compared with controls (P = .0002). Conversely, saliva EGFR concentrations were higher in OSCC patients than in controls (P = .0014). Saliva EGFR levels were also increased in patients with higher T category (pT4 vs. pT <4, median 6.0 vs. 3.8 ng/mL, P = .02). Considering 9.2 ng/mL (fourth quartile) as the cutoff value, patients with higher levels of saliva EGFR had worse prognosis in terms of overall survival (P = .04). Conversely, no association was found between serum EGFR and clinical outcomes in OSCC patients.ConclusionsSaliva EGFR can be considered as a potential tumor marker for OSCC with both diagnostic and prognostic values. Serum EGFR levels, on the other hand, were lower in OSCC patients, but did not show any prognostic impact. Saliva EGFR levels are worthy of further investigation as a potential diagnostic and prognostic biomarker for OSCC.Level of Evidence3b. Laryngoscope, 127:E408–E414, 2017
Selective patient experience with the Blom‐Singer Dual Valve voice prosthesis
Authors: Brownlee Ben , Ahmad Saniya , Grammer Tracy , Krempl Greg 
Published: August 2017

Objectives/HypothesisThis study compares the overall durability, phonation effort, and speech quality achieved by the Blom‐Singer Dual Valve prosthesis to the same characteristics achieved by the single‐valve prosthesis used in a select population of total laryngectomy patients who have demonstrated single‐valve prosthesis failure within 3 months of placement.Study DesignProspective phase IV study.MethodsFourteen patients who had previously experienced early valve failure using a single‐valve prosthesis had a Dual Valve placed by a speech language pathologist. A total of seventeen Dual Valves were inserted during routine clinical visits for valve replacement. Time intervals from insertion to replacement of the Dual Valve, as well as the average lifespan of patients’ three previous single valves were collected. Subjective, categorical responses to speech quality and phonation effort for the Dual Valve compared to the single valve were collected on a questionnaire upon failure of the device.ResultsThe mean duration of the single valve was 60 days, with a median of 51 days, whereas the mean duration of the Dual Valve was 164 days, with a median duration of 84 days. Both sets of data were statistically significant, with a P value of .0131. Valve life was increased in 86% of patients, with 43% experiencing greater than 150 days improvement in valve duration when using the Dual Valve. Phonation effort was increased in 14% of patients, and speech quality was not significantly affected in any patients.ConclusionsIn this select population, the Dual Valve offers improved durability over the single valve without sacrificing speech quality, making it a preferred prosthesis option for patients experiencing single‐valve failure in less than 3 months.Level of Evidence4. Laryngoscope, 128:422–426, 2018
Optical coherence tomography image‐guided smart laser knife for surgery
Authors: Katta Nitesh , McElroy Austin B. , Estrada Arnold D. , Milner Thomas E. 
Published: August 2017

Background and ObjectiveSurgical oncology can benefit from specialized tools that enhance imaging and enable precise cutting and removal of tissue without damage to adjacent structures. The combination of high‐resolution, fast optical coherence tomography (OCT) co‐aligned with a nanosecond pulsed thulium (Tm) laser offers advantages over conventional surgical laser systems. Tm lasers provide superior beam quality, high volumetric tissue removal rates with minimal residual thermal footprint in tissue, enabling a reduction in unwanted damage to delicate adjacent sub‐surface structures such as nerves or micro‐vessels. We investigated such a combined Tm/OCT system with co‐aligned imaging and cutting beams—a configuration we call a “smart laser knife.”MethodsA blow‐off model that considers absorption coefficients and beam delivery systems was utilized to predict Tm cut depth, tissue removal rate and spatial distribution of residual thermal injury. Experiments were performed to verify the volumetric removal rate predicted by the model as a function of average power. A bench‐top, combined Tm/OCT system was constructed using a 15W 1940 nm nanosecond pulsed Tm fiber laser (500 μJ pulse energy, 100 ns pulse duration, 30 kHz repetition rate) for removing tissue and a swept source laser (1310 ± 70 nm, 100 kHz sweep rate) for OCT imaging. Tissue phantoms were used to demonstrate precise surgery with blood vessel avoidance. Depth imaging informed cutting/removal of targeted tissue structures by the Tm laser was performed.ResultsLaser cutting was accomplished around and above phantom blood vessels while avoiding damage to vessel walls. A tissue removal rate of 5.5 mm3/sec was achieved experimentally, in comparison to the model prediction of approximately 6 mm3/sec.ConclusionWe describe a system that combines OCT and laser tissue modification with a Tm laser. Simulation results of the tissue removal rate using a simple model, as a function of average power, are in good agreement with experimental results using tissue phantoms. Lasers Surg. Med. 50:202–212, 2018. © 2017 Wiley Periodicals, Inc.
Altered behavioral responses to gamma‐aminobutyric acid pharmacological agents in a mouse model of Huntington's disease
Authors: Hsu Yi‐Ting , Chang Ya‐Gin , Chang Ching‐Pang , Siew Jian‐Jing , Chen Hui‐Mei , Tsai Chon‐Haw , Chern Yijuang 
Published: August 2017

ABSTRACTBackground: Disruptions in gamma‐aminobutyric (GABA) acid signaling are believed to be involved in Huntington's disease pathogenesis, but the regulation of GABAergic signaling remains elusive. Here we evaluated GABAergic signaling by examining the function of GABAergic drugs in Huntington's disease and the expression of GABAergic molecules using mouse models and human brain tissues from Huntington's disease.Methods: We treated wild‐type and R6/2 mice (a transgenic Huntington's disease mouse model) acutely with vehicle, diazepam, or gaboxadol (drugs that selectively target synaptic or extrasynaptic GABAA receptors) and monitored their locomotor activity. The expression levels of GABAA receptors and a major neuron‐specific chloride extruder (potassium‐chloride cotransporter‐2) were analyzed by real‐time quantitative polymerase chain reaction, Western blot, and immunocytochemistry.Results: The R6/2 mice were less sensitive to the sedative effects of both drugs, suggesting reduced function of GABAA receptors. Consistently, the expression levels of α1/α2 and δ subunits were lower in the cortex and striatum of R6/2 mice. Similar results were also found in 2 other mouse models of Huntington's disease and in Huntington's disease patients. Moreover, the interaction and expression levels of potassium‐chloride cotransporter‐2 and its activator (brain‐type creatine kinase) were decreased in Huntington's disease neurons. These findings collectively suggest impaired chloride homeostasis, which further dampens GABAA receptor‐mediated inhibitory signaling in Huntington's disease brains.Conclusions: The dysregulated GABAergic responses and altered expression levels of GABAA receptors and potassium‐chloride cotransporter‐2 in Huntington's disease mice appear to be authentic and may contribute to the clinical manifestations of Huntington's disease patients. © 2017 International Parkinson and Movement Disorder Society
MRI assessment of coronary microvascular endothelial nitric oxide synthase function using myocardial T1 mapping
Authors: Cui Sophia X. , Epstein Frederick H. 
Published: August 2017

PurposeEndothelial nitric oxide synthase (eNOS) plays a central role in regulating vascular tone, blood flow, and microvascular permeability. Endothelial dysfunction, including eNOS dysfunction, is an early biomarker of vascular disease. This study aimed to show that myocardial T1 mapping during nitric oxide synthase (NOS) inhibition could assess coronary microvascular eNOS function.MethodsWild‐type mice, eNOS−/− mice, and wild‐type mice fed a high‐fat diet underwent T1 mapping at baseline and for 20 min after injection of NG‐nitro‐L‐arginine methyl ester (LNAME), a NOS inhibitor. First‐pass perfusion MRI was performed in wild‐type mice at baseline and 5 min after LNAME injection.ResultsT1 mapping detected an increase in myocardial T1 5 min after an injection of 4 mg/kg LNAME compared with baseline in control mice (T1 = 1515 ± 30 ms with LNAME versus T1 = 1402 ± 30 ms at baseline, P < 0.05). No change in myocardial T1 after LNAME injection was observed in eNOS−/− mice. The change in T1 after LNAME injection was less in high‐fat‐diet mice (ΔT1 = 31 ± 14 ms at 12 weeks of diet and ΔT1 = 16 ± 17 ms at 18 weeks of diet) compared with mice fed a standard diet (ΔT1 = 113 ± 15 ms), with P < 0.05. First‐pass MRI measured similar perfusion at baseline and 5 min after LNAME injection.ConclusionsNOS inhibition causes an increase in myocardial T1 in healthy mice, and this effect is mediated through eNOS. T1 mapping during NOS inhibition detects coronary microvascular eNOS dysfunction in high‐fat‐diet mice. T1 mapping during NOS inhibition may be useful in preclinical studies aiming to investigate mechanisms underlying and therapies for coronary microvascular eNOS dysfunction. Magn Reson Med 79:2246–2253, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
Improvements in Cardiovascular Risk Factors in Young Adults in a Randomized Trial of Approaches to Weight Gain Prevention
Authors: Wing Rena R. , Tate Deborah F. , Garcia Katelyn R. , Bahnson Judy , Lewis Cora E. , Espeland Mark A. ,   for the Study of Novel Approaches to Weight Gain Prevention (SNAP) Research Group
Published: August 2017

ObjectiveWeight gain occurs commonly in young adults and increases the risk of cardiovascular disease (CVD). Following on a previous report that two self‐regulation interventions reduced weight gain relative to control, this study examines whether these interventions also benefit CVD risk factors.MethodsThe Study of Novel Approaches to Weight Gain Prevention was a randomized trial in two academic settings (N = 599; 18‐35 years; BMI 21‐30 kg/m2) comparing two interventions (Self‐Regulation with Small Changes; Self‐Regulation with Large Changes) and a control group. Small Changes taught participants to make daily small changes in calorie intake (approximately 100 calories) and activity. Large Changes taught participants to initially lose 5 to 10 pounds to buffer anticipated weight gains. CVD risk factors were assessed at baseline and at 2 years in 471 participants.ResultsAlthough Large Changes was associated with more beneficial changes in glucose, insulin, and homeostatic model assessment of insulin resistance than Control, these differences were not significant after adjusting for multiple comparisons or 2‐year weight change. Comparison of participants grouped by percent weight change from baseline to 2 years showed significant differences for several CVD risk factors, with no interaction with treatment condition.ConclusionsMagnitude of weight change, rather than specific weight gain prevention interventions, was related to changes in CVD risk factors in young adults.
Specific Binding of Liposomal Nanoparticles through Inverse Electron‐Demand Diels–Alder Click Chemistry
Authors: Brand Christian , Iacono Pasquale , Pérez‐Medina Carlos , Mulder Willem J. M. , Kircher Moritz F. , Reiner Thomas 
Published: August 2017

AbstractHere, we report a method to specifically bind liposomal radiopharmaceuticals to a CoCrMo alloy, which can be used in arterial stents, via an irreversible inverse electron‐demand Diels–Alder reaction. Inspired by recent accomplishments in pre‐targeted imaging using tetrazine‐trans‐cyclooctene click chemistry, we synthesized 89Zr‐labeled trans‐cyclooctene‐functionalized liposomal nanoparticles, which were validated on a tetrazine‐appended polydopamine‐coated CoCrMo surface. In efforts to ultimately translate this new material to biomedical applications, we compared the ability of 89Zr‐TCO–liposomal nanoparticles (89Zr‐TCO‐LNP) to be immobilized on the tetrazine surface to the control suspensions of non‐TCO functionalized 89Zr‐liposomal nanoparticles. Ultimately, this platform technology could result in a systemic decrease of the radiotherapeutic dose deposited in non‐targeted tissues by specific removal of long‐circulating liposomal radiopharmaceuticals from the blood pool.
Detonation Performance Characterization of a Novel CL‐20 Cocrystal Using Microwave Interferometry
Authors: Vuppuluri Vasant S. , Samuels Philip J. , Caflin Kelley C. , Gunduz I. Emre , Son Steven F. 
Published: August 2017

AbstractDevelopment of novel energetic materials is a significant challenge. Cocrystallization has been explored as another route to development of novel materials. However, very little characterization of detonation performance has been performed for these energetic cocrystals. A major challenge for performing detonation velocity measurements with cocrystals is that typical measurement techniques require hundreds of grams to kilograms of material, an amount that exceeds the entire supply of many cocrystals. In this work, small‐scale detonation velocity measurements using about 1.2 g of material per test employing microwave interferometry are presented and discussed for a novel cocrystal of 1‐methyl‐3,5‐dinitro‐1,2,4‐triazole (MDNT) and hexanitrohexaazaisowurtzitane (CL‐20) in a 1 : 1 molar ratio and compared to a physical mixture of MDNT and CL‐20 in the same molar ratio. The results are compared with detonation velocity measurements with cyclotetramethylene tetranitramine (HMX), which provide validation of the technique and further comparison of the results. With this technique, detonation velocity differences as low as 100 m/s are resolvable. The MDNT/CL‐20 cocrystal is observed to detonate over 500 m/s faster than the physical mix and over 600 m/s faster than HMX at the same charge density which is held constant in this work. The enthalpy of formation of the MDNT/CL‐20 cocrystal was also measured. Using this, the detonation velocity of the cocrystal was calculated using thermochemistry to be 230 m/s faster than that of the physical mixture of MDNT and CL‐20 in the same molar ratio as is contained within the cocrystal at a charge density of 1.4 g/cm3. The higher detonation velocity of the cocrystal (both measured and predicted) compared to the physical mixture is likely attributable to bonding energy contained within the cocrystal and the arrangement of the coformers within the cocrystal.
Low Doses of Curcuma longa Modulates Cell Migration and Cell–Cell Adhesion
Authors: de Campos Paloma Santos , Matte Bibiana Franzen , Diel Leonardo Francisco , Jesus Luciano Henrique , Bernardi Lisiane , Alves Alessandro Menna , Rados Pantelis Varvaki , Lamers Marcelo Lazzaron 
Published: August 2017

Cell invasion and metastasis are involved in clinical failures in cancer treatment, and both events require the acquisition of a migratory behavior by tumor cells. Curcumin is a promising natural product with anti‐proliferative activity, but its effects on cell migration are still unclear. We evaluated the effects of curcumin on the proliferation, apoptosis, migration, and cell–cell adhesion of keratinocyte, oral squamous cell carcinoma (OSCC), and fibroblast cell lines, as well as in a xenograft model of OSCC. Curcumin (2 μM) decreased cell proliferation in cell lines with mesenchymal characteristics, while cell death was detected only at 50 μM. We observed that highly migratory cells showed a decrease on migration speed and directionality when treated with 2 or 5 μM of curcumin (50% and 40%, respectively, p < 0.05). Using spheroids, we observed that curcumin dose dependently decreased cell–cell adhesion, especially on tumor‐derived spheroids. Also, in a xenograft model with patient‐derived OSCC cells, the administration of curcumin decreased tumor growth and aggressiveness when compared with untreated tumors, indicating the potential antitumor effect in oral cancer. These results suggest that lower doses of curcumin can influence several steps involved in tumorigenesis, including migration properties, suggesting a possible use in cancer therapy. Copyright © 2017 John Wiley & Sons, Ltd.
The Preparation of Hyaluronic Acid Nanoparticles from Aspicilia lichens Using Bifido Bacteria for Help in the Treatment of Diabetes in Rats In Vivo
Authors: Esmaeili Akbar , Rajaee Shirin 
Published: August 2017

Many common herbs and spices are claimed to have blood sugar lowering properties that make them useful for people with or at high risk of diabetes. The main of compounds of kiwifruit (Actinidia deliciosa), rhubarb (Rheum ribes), membrane inner of egg shell, wool of sheep, human fingernail (unguis), hyaluronic acid produced by the Bifidobacterium, and usnic acid of Aspicilia lichen were extracted by different methods. All compounds of the extract were divided into five groups. We used variables such as pH, different compounds, concentration, number of injections, and blood glucose monitoring in different situations. Our study, extracts changed to nanoform. The extract compounds and nanoparticles were analyzed by gas chromatography mass spectroscopy, Fourier transform infrared spectroscopy, hydrogen‐1 nuclear magnetic resonance, and scanning electron microscope. The average size of the nanoparticles was found to be 55 nm. Five groups of nanoparticles were injected into rats, and they reduced their blood glucose levels significantly (statistical significance was declared at p < 0.05). The synthesized hyaluronic acid helped to treat diabetes in rats. Copyright © 2017 John Wiley & Sons, Ltd.