bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENINGKATAN SINTASAN DAN KETAHANAN LARVA UDANG WINDU (Penaeus nxonodon) MELALUI PENAMBAHAN BAKTERI Vibrio harveyi KE DALAM PAKAN MIKRO
Authors: Zafran Zafran , Roza Des , Suwirya Ketut 
Published: April 2017

Vibriosis merupakan penyakit bakteria yang dihadapi para pengelola hatcheri dalam memproduksi benih udang. Vaksinasi diyakini merupakan suatu metode yang efektif untuk meningkatan ketahanan udang terhadap infeksi bakteri. Di Loka Penelitian Perikanan Pantai Gondol.
Evidencia genética revela oportunidades de mejora para preparación de tejidos en análisis forenses
Authors: Romero Rosa Elena , Sandoval Alejandro , Arango Juliana , Camargo Martha Lucia 
Published: April 2017

Introducción: los tejidos embebidos en parafina constituyen una excelente alternativa para obtener adn, especialmente cuando no es posible contar con muestras frescas o el almacenamiento y conservación del tejido no es viable, de manera que esta muestra es el único elemento disponible para realizar un cotejo. El éxito en cualquier análisis genético es contar con métodos de fijación de tejidos y de extracción de adn adecuados que permitan obtener moléculas de buena calidad y cantidad, libres de contaminantes biológicos, químicos y microbiológicos que pueden actuar como inhibidores en los procesos de amplificación por pcr. Métodos y materiales: en el laboratorio de genética del Instituto Nacional de Medicina Legal y Ciencias Forenses, Regional Suroccidente, Colombia, se realizó una prueba preliminar para la validación de la extracción de adn en tejidos embebidos en parafina, utilizando el método orgánico validado y empleado de rutina por ellaboratorio de genética de Bogotá, Colombia. Resultados y discusión: se corroboró la eficacia del método a partir del análisis de reproducibilidad, respetabilidad y concordancia en cuanto a concentración de adn, y obtención de perfiles genéticos a partir de los extractos de adn de cuatro réplicas de tres diferentes tipos de tejidos obtenidos a partir de un feto, con respecto a una muestra de sangre en soporte fta. Conclusiones: con los resultados preliminares obtenidos, se encontraron algunas oportunidades de mejora para los laboratorios que intervienen en el proceso.
Role of conservative treatment of chronic venous insufficiency for regression of the features of desadaptive cardiac remodeling in patients with comorbid obesity
Authors: Leukhnenko I N , Teplyakova O V , Grishina I F 
Published: April 2017

Aim. To evaluate the influence of conservative treatment tactics for chronic venous insufficiency on the processes of cardiac remodeling in patients with comorbid obesity.
Methods. 32 males with obesity class 1 (BMI 32.57±2.25 kg/m2) at the age of 40.7±11.5 years and with clinical and instrumental signs of chronic venous insufficiency were divided into 2 groups: patients from group 1 (n=14) followed the recomendations to wear compression stockings and to use hesperidin + diosmin 500 mg twice a day, and the patients from group 2 (n=18) did not follow those recommendations. All patients had echocardiography performed before the treatment and 2 months after the first visit. For the evaluation of echocardiographic dynamics paired Student’s t-test was used.
Results. After 2 months statistically significant dynamics of the following echocardiographic findings was revealed: in group 1 index of eccentric left ventricular shape increased from 0.94±0.06 to 1.01±0.03, in group 2 - from 0.96±0.08 to 0.97±0.07 (р=0.023). In group 1 tricuspid annular plane systolic excursion decreased from 30.07±5.31 to 26.64±4.14 mm, in group 2 - from 29.6±4.72 to 29.88±6.04 mm (р=0.008), and the right atrial volume in group 1 decreased from 53.57±11.48 to 46.64±13.18 ml, in group 2 - from 52.77±11.27 to 51.38±8.54 ml (р=0.044). In group 1 statistically significant trend to decreased ratio of maximal velocity of RV early diastolic filling to maximum diastolic tricuspid valve lateral annular velocity was revealed (from 4.75±1.35 to 4.25±1.23 cm/sec) compared to group 2 (from 4.79±1.62 to 4.75±1.58 cm/sec; р=0.076).
Conclusion. Conservative treatment of chronic venous insufficiency in patients with obesity is associated with indirect indicators of decreasing volume overload of the heart and can influence positively on heart geometry leading to regression of spheric LV remodeling and decrease of RA volume.
Features of platelet reaction in acute pancreatitis
Authors: Chikaev V F , Aydarov A R , Zinkevich O D , Petukhov D M , Galyautdinov F S , Safina N A 
Published: April 2017

Aim. To identify the features of platelet reaction in peripheral blood in patients with acute pancreatitis.
Methods. We conducted a retrospective analysis of changes of platelets concentration in 19 patients with acute pancreatitis admitted to Kazan City clinical hospital №7. The age of patients ranged from 35 to 76 years, 45±12 years in average. All patients received complex treatment. The main method of surgical treatment was navigation drainage of fluid collections, peripancreatic mass and retroperitoneal space under ultrasound control performed in 11 patients. Subsequently in 4 patients due to progressive pyoinflammatory process midline laparotomy, sanitation, drainage of omental sac and purulent cavities were performed. In 4 cases aseptic pancreonecrosis resolved after conservative treatment. All patients dynamically had erythrocytes and platelets count and mean platelet volume checked.
Results. Analysis of platelets concentration in peripheral blood in patients with acute pancreatitis showed multidirectional character depending on the severity of pathological process. In mild acute pancreatitis the platelet count remains within normal limits. In severe pancreatitis its number increases. The highest numbers of platelets were observed on days 10-20. On discharge platelet count remained elevated. Severe pervasive pancreonecrosis (n=4) with fatal outcome was characterized by thrombocytopenia on admission with further progression.
Conclusion. The nature of the changes in platelet reaction in patients with acute pancreatitis depends on the severity of the pathological process and can be used as a criterion for evaluation of treatment effectiveness and prognosis of the outcome of the disease.
Clinical and pathogenetic features of congenital heart disease in newborns with intrauterine infection
Authors: Beniova S N , Figol’ S Yu , Kornilova O A , Stolina M L , Blokhina N P 
Published: April 2017

Aim. Investigation of the features of diagnosis and treatment of congenital heart disease in newborns with intrauterine herpes and cytomegalovirus infections.
Methods. A comparative analysis of risk factors, clinical signs and dynamics of serum levels of matrix metalloproteinases in 91 newborns with congenital heart disease during the early neonatal period was performed. All patients were divided into 2 groups: group 1 (study group) - 51 patients with congenital heart disease and intrauterine Herpes or Cytomegalovirus infection, group 2 (comparison group) - 40 children with heart disease without the signs of intrauterine infection. The control group consisted of 20 relatively healthy full-term newborns.
Results. Mothers of children with congenital heart disease and intrauterine infection more often compared to mothers of children from group 2 had inflammatory disease of reproductive system (49.1 and 22.5% respectively, р ≤0.05), medical abortions in past medical history (84.3 and 27.5% respectively, р ≤0.01), acute respiratory viral infections during pregnancy (21.6 and 7.5% respectively, р ≤0.05). Levels of matrix metalloproteinase 9 in umbilical blood plasm were statistically significantly increased in all newborns with congenital heart disease compared to control levels. The highest concentration of enzyme was registered in patients with congenital heart disease and nervous system disorders associated with intrauterine infection and in newborns with severe heart disease. Inflammatory extracardial changes in patients with congenital heart disease associated with intrauterine infection caused by Herpes Simplex virus and Cytomegalovirus were accompanied by significant increase of matrix metalloproteinase 8.
Conclusion. The association between matrix metalloproteinase 9 in blood serum of newborns and clinical signs of a congenital heart disease during early neonatal period was revealed; high levels of metalloproteinase 8 can indicate intrauterine infection in newborns with congenital heart disease during early neonatal period.
Improvement of dental health in professional athletes engaged in power sports
Authors: Safaraliev F R , Safarov A M , Mamedov F Yu 
Published: April 2017

Aim. Evaluation of dental diseases prevalence in professional athletes engaged in power sports and of the influence of biologically neutral products based on propolis on the level of their dental health.
Methods. The examination of organs and tissues of the oral cavity of professional athletes engaged for a long time in power sports was carried out. With the use of the developed specialized questionnaire dental status of the athletes was determined. Based on the analysis of the results of clinical and laboratory studies anti-inflammatory and anti-bacterial effectiveness of products based on propolis were evaluated.
Results. The results of the survey showed that not all athletes are informed about the possibility of aggravation of pathological processes in soft and hard tissues of oral cavity during active training, mainly, in power sports. Lack of awareness of surveyed athletes about the need for timely individual oral hygiene procedures and regular dentist visits was revealed, especially during intense training before and after competitions. On evaluation of dental status of professional combat athletes high prevalence and intensity of inflammatory periodontal disease and caries were found. High anti-inflammatory efficiency of the product «Pomegranate Balm» for correction of dental status of professional athletes was proven.
Conclusion. High incidence of inflammatory periodontal diseases of mild to moderate severity was revealed in athletes during prolonged and intense physical training; efficacy of biologically neutral products based on propolis was established.
Characteristics of dental status of patients with inflammatory periodontal diseases
Authors: Vasil’eva N A , Bulgakova A I , Soldatova E S 
Published: April 2017

Aim. Evaluation of dental status in patients with inflammatory periodontal diseases.
Methods. The study of dental status was performed with the use of clinical and historical data from 269 patients with inflammatory periodontal diseases at the age of 18-65 years. Among examined patients there were 75 (27.9%) people with gingivitis, 54 (20.1%) with mild periodontitis, 66 (24.5%) with moderate and 74 (27.5%) with severe periodontitis. Control group consisted of practically healthy donors at the age of 18-52 years (40 people) with sanitized oral cavity.
Results. From history and interviewing it was found that 72% of the surveyed patients brush their teeth twice a day, 24% - once a day and 4% of patients do not brush their teeth. Family history of periodontal diseases among parents was recorded by 86% of participants. Dental status was characterized by the increase of the values of all dental indices with increasing disease severity regardless of gender. Need for correction of the depth of the vestibule, lips, cords, and bridles was identified in 51.7% of cases in patients with gingivitis and in 96.6% of cases of periodontal diseases. With increasing severity of periodontitis index (sum) of teeth with decay and fillings and removed teeth was increased by 1.5 times in gingivitis, by 2.2 times in mild periodontitis, by 2.6 times in moderate and by 2.9 times in severe periodontitis compared to control group. Partial adentia in gingivitis is 3 times more prevalent in males than in females and in severe periodontitis is 1.6 times more prevalent in females than in males.
Conclusion. Dental status of the patients with inflammatory periodontal diseases was characterized by increased values of all dental indices compared to control group that demonstrates typical course of inflammatory periodontal diseases and decrease of local immunologic resistance of oral cavity and the organism in total; timely correction of local factors (local trauma), anatomical and topographical features of dentofacial system and status of oral soft tissues allows prevention of pathologic processes in periodontal tissues.
Evaluation of the possibility to use calcium channel blockers for prevention of intra-abdominal adhesions formation
Authors: Sokolova T F , Skal’skiy S V , Turok N E 
Published: April 2017

Aim. To study the possibility of the use of known medications used in cardiology - calcium channel blockers - derivatives of phenylalkilamine (verapamil), benzodiazepine (diltiazem), dihydropyridine (nifedipine) - for prevention of peritoneal adhesion formation.
Methods. The study of the effect of different concentrations of medications on nuclear translocation of NF-κB, proliferative and collagen-synthetic activity of fibroblasts was conducted in the primary culture of rats peritoneal fibroblasts with hemoperitoneum. Aseptic injury of peritoneum was caused by modeling of autohemoperitoneum according to original method.
Results. It was found that verapamil and diltiazem normalized nuclear translocation of NF-κB, processes of fibroblasts proliferation and their collagen synthesis by optimizing fibroblast functional activity during adhesion formation induction. Verapamil and diltiazem affected fibroblasts similarly but with different intensity. Verapamil in concentrations 0.05 and 0.1 mg/ml and diltiazem in concentration 0.1 mg/ml suppressed the excessive fibroblast activity by inhibiting their proliferative and collagen-synthetic activity, nuclear translocation of NF-κB. Verapamil demonstrated the most prominent pharmacological effect. Nifedepine did not reveal such effect. At the molecular level inhibiting effect of calcium channel blockers verapamil and diltiazem on the activation of apoptosis blocking nuclear transcription factor NF-κB was demonstrated.
Conclusion. Verapamil and diltiazem can be recommended for further detailed experimental and clinical studies focused on widening of biological activity spectrum of known medications and area of their clinical use.
Impact of resource provision for the regional infection services on public infectious morbidity
Authors: Galieva A M , Vafin A Y , Kravchenko I I 
Published: April 2017

Aim. To study the impact of resource provision for the regional infection services on public infectious morbidity rate and determine priority ways of the service development.
Methods. The analysis of infectious diseases statistics by the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being office in the Republic of Tatarstan as well as 2005-2015 annual reports of infection service of the Ministry of Health Care of the Republic of Tatarstan was carried out using statistical methods.
Results. Public infectious morbidity in the Republic of Tatarstan increased by 11.7% during the period of 2005-2015, which indicates low growth rate. The average level of infectious morbidity variation was revealed (the coefficient of variation - 11.24%). During the analyzed period (2005-2015) in order to optimize infection service of the Republic of Tatarstan resource provision of the infection service (number of infectiologists, bed capacity, offices of infectious diseases) was cut. A significant correlation between infectious morbidity and resource provision for the service at the level of municipal districts was found. Priority ways of Tatarstan service development are creation of the Center for Infectious Pathology and introduction of new forms of examination and treatment. Low growth rate and average level of infectious morbidity variation in the Republic of Tatrstan during the period of 2005-2015 were revealed. A significant correlation between infectious morbidity and resource provision of the service was found. The highest infectious morbidity rate was registered in large cities of the Republic with high availability of human resources, bed capacity and specialized laboratories, while low morbidity rate was registered at municipal level with lack of infectiologists, subdivisions of infection service and specialized laboratories.
Conclusion. Reduction of material and technical resources and human resources of infection service of the Republic of Tatarstan had an impact on infectious morbidity rate in the region associated with decreased level of diagnosis of infectious diseases at certain territories.
Epidemiological analysis of neonatal infectious morbidity associated with health care
Authors: Zaripova A Z , Badamshina G G , Ziatdinov V B , Isaeva G Sh 
Published: April 2017

Aim. Investigation of the features of epidemiological characteristics of infectious morbidity associated with medical care registered in newborns in maternity care facilities of the Republic of Tatarstan.
Methods. Retrospective epidemiological study of the morbidity of infections associated with medical care registered in newborns in the Republic of Tatarstan was performed. Epidemiological analysis was carried out using the forms of Federal statistical observation №2 of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing «The information about the infectious and parasitic diseases» (section «Hospital-acquired infections»).
Results. Based on the received materials epidemiological analysis of the dynamics of hospital-acquired infectious morbidity in newborns for the period from 2009 to 2015 was performed. Unidirectional trend of infectious morbidity associated with medical care and of various nosological forms was revealed. Downward trend with a negative morbidity growth rate was identified for neonatal pneumonia, osteomyelitis, pyoderma, impetigo, mastitis, panaritium and paronychia of newborns, omphalitis, phlebitis of the umbilical vein and neonatal conjunctivitis. Neonatal morbidity of hospital-acquired otitis media did not changed from the baseline. Prevalent nosological forms of infections associated with medical care among newborns were pyoderma, impetigo, mastitis, panaritium, paronychia (33.6%) and pneumonia (29.5%).
Conclusion. The average morbidity of infections associated with medical care in newborns in the Republic of Tatarstan for the period from 2009 to 2015 was 13.0 per 1000 newborns; negative dynamics of the morbidity with negative morbidity growth rate in newborns during this period was revealed (-42.3%).
Alcohol intoxication: evaluation of the population appealability and emergency medical care in Kazan
Authors: Paykov V L , Zamaleeva E I , Zhukov D A , Chernova O L 
Published: April 2017

Aim. To study population appealability for emergency medical care with alcohol intoxication as well as the features of medical care service for them in Kazan at modern stage.
Methods. The data from emergency call cards from 2015 with the diagnosis «alcohol intoxication» (form No.11/u) were studied. A survey of 271 responders (medical personnel of mobile teams of emergency care and admission departments of the hospitals) of medical care service for people with alcohol intoxication in the streets was performed.
Results. In the structure of performed calls for adult popultion the ratio of patients who called an ambulance because of alcohol intoxication was 2.1% and because of the need for urgent care - 5.7%. Males were more prevalent than females: 82.1 and 17.9% respectively. Predominantly people younger than 60 years appealed: among males 82.7%, among females - 79%. Maximum appealability was registered in July (7.4 calls per 10 000 adults); during the week - on Saturday (11.9 per 10 000 adults), and during the day - during the period from 5 to 6 pm. The survey of the teams of ambulances and admission departments demonstrated the need for re-establishment of medical sobering-up stations (83.5 and 80% respectively) and more rarely the responders suggested development of specialized medical departments and active delivery of people with alcohol intoxication to specialized institutions involving law enforcement officials and personnel of specialized sobering-up stations (13 and 14.3% respectively).
Conclusion. In the structure of the calls performed by emergency care stations the ratio of patients who called an ambulance because of alcohol intoxication among adults was 2.1% and because of the need for urgent care - 5.7%; the appealability was affected by sex, age and calendar time; analysis of the survey results demonstrated the need for re-establishment of recently closed medical sobering-up stations and for development of specialized medical departments.
Technology of liquid chromatographic serum spectral images fixation in the diagnosis of colonic mucosal dysplasia
Authors: Alekseeva O P , Kolodey E N 
Published: April 2017

Aim. To demonstrate the possibility of use of technology of liquid chromatographic serum spectral images fixing for the diagnosis of colonic epithelial dysplasia in patients with ulcerative colitis.
Methods. 49 patients with ulcerative colitis were examined. Colonoscopy with Mayo index evaluation and biopsy of the intestinal mucosa were carried out using OLYMPUS CV-170 device (Japan). Biopsies were taken from the most affected areas of the colonic mucosa (from 3 to 10 biopsies). Morphological study included an assessment of histological activity of ulcerative colitis and colonic mucosal dysplasia. The results of histological studies were evaluated by two experts. High performance liquid chromatography of serum was performed by a standard technique in the chromatograph «Milichrom A-02» (CJSC «EcoNova», Novosibirsk). Statistical analysis of serum chromatograms was performed with the use of chromatograph combined with a PC, the end result was construction of spectral images of the disease.
Results. In 13 patients with ulcerative colitis the results of histological examination revealed various degrees of dysplasia. The set of spectral images of different patients, obtained in the processing of chromatograms, composed the diagnostic cloud of a pathological condition. The study revealed complete differentiation of liquid-chromatographic spectral images of serum of patients with ulcerative colitis with and without colonic mucosal dysplasia according to the results of multivariate cluster analysis. The diagnostic accuracy of the method was 88%.
Conclusion. The possibility of using the technology of construction and analysis of liquid-chromatographic spectral images of serum for the diagnosis of colonic epithelial dysplasia in patients with ulcerative colitis was demonstrated.