bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing best practice guidelines for designing living environments for people with dementia and sight loss
Authors: BOWES ALISON , DAWSON ALISON , GREASLEY-ADAMS CORINNE , MCCABE LOUISE 
Published: January 2017

ABSTRACTThe paper considers a process of developing evidence-based design guidelines to be used in environments where people with dementia and sight loss are living. The research involved a systematically conducted literature review and a series of consultations with people affected by dementia and/or sight loss who lived or worked in care homes or in domestic settings. Findings from the literature and the consultations were used in an iterative process to develop the guidelines. The process is outlined, providing examples from the guidelines about lighting and colour and contrast. In discussing the research findings and the development process, the authors consider implications of the work including the weakness of the evidence base, the challenges of improving this and the need for innovative approaches to understanding the complexities of design for people with dementia and sight loss. They highlight the emphasis in the literature on independence for people with sight loss and the focus on control of people with dementia, arguing that this falls short of a genuinely person-centred approach, which recognises the active participation of people with dementia and sight loss.
Living with Parkinson's disease – perceptions of invisibility in a photovoice study
Authors: ROGER KERSTIN , WETZEL MONIKA , PENNER LESLIE 
Published: January 2017

ABSTRACTBased on previous community research, we prioritise the need to understand better how experiences and feelings of invisibility could be shaped by relational dynamics, interfacing with service provision, and social forces, with the overarching view of understanding better the experiences of participants living with Parkinson's disease. A photovoice methodology was employed. A discussion of one case study of Bindy and Volta leads to a better understanding of how strong spousal support can significantly alter how one individual experiences and defines living with Parkinson's.
The SESAME materials science beamline for XRD applications
Authors: Abdellatief M. , Rebuffi L. , Khosroabadi H. , Najdawi M. , Abu-Hanieh T. , Attal M. , Paolucci G. 
Published: January 2017

We present a detailed description of the SESAME Materials Science (MS) beamline for X-ray diffraction (XRD) applications, presently under construction in Allan, Jordan. The beamline is based on components previously installed at the Swiss Light Source, but modifications in the beamline design have been introduced to match the characteristics of the SESAME storage ring. The SESAME MS beamline will accommodate XRD experiments in the energy range between 5 and 25 keV. The beamline ray tracing analysis at 10 keV estimates the flux at the sample to be of the order of 1013 (photons s−1), the energy resolution is about 2 eV and the effective beam size at the sample of 300 × 2800 µm2. Investigations of microstruture will be possible as the instrumental broadening, resulted from simulating the diffraction pattern for a standard material, is of the order of 0.01° at 15 keV. A wide range of applications will be possible at the beamline, such as powder diffraction studies, single crystals and in situ XRD. The commisioning of the beamline is expected to be in the second half of 2017.
Challenges in the industrial implementation of generative design systems: An exploratory study
Authors: Nordin Axel 
Published: January 2017

AbstractThe aim of this paper is to investigate the challenges associated with the industrial implementation of generative design systems. Though many studies have been aimed at validating either the technical feasibility or the usefulness of generative design systems, there is, however, a lack of research on the practical implementation and adaptation in industry. To that end, this paper presents two case studies conducted while developing design systems for industrial uses. The first case study focuses on an engineering design application and the other on an industrial design application. In both cases, the focus is on detail-oriented performance-driven generative design systems based on currently available computer-assisted design tools. The development time and communications with the companies were analyzed to identify challenges in the two projects. Overall, the results show that the challenges are not related to whether the design tools are intended for artistic or technical problems, but rather in how to make the design process systematic. The challenges include aspects such as how to fully utilize the potential of generative design tools in a traditional product development process, how to enable designers not familiar with programming to provide design generation logic, and what should be automated and what is better left as a manual task. The paper suggests several strategies for dealing with the identified challenges.
Characterizing innovative processes in design spaces through measuring the information entropy of empirical data from protocol studies
Authors: Kan Jeff W.T. , Gero John S. 
Published: January 2017

AbstractThis paper reports on a study characterizing design processes and the potential of design spaces through measuring the information entropy of empirical data derived from protocol studies. The sequential segments in a protocol analysis can be related to each other by examining their semantic content producing a design session's linkograph, which defines the design space for a design session. From a linkograph, it is possible to compute the probabilities of the connectivity of each segment for its forelinks and its backlinks, together with the probabilities of distance among links. A linkograph's entropy is a measure of the information in the design session. It is claimed that the entropy of the linkograph measures the potential of the design space being generated as the design proceeds chronologically. We present an approach to the automated construction of linkographs by connecting segments using the lexical database WordNet and measure its entropy. A case study of two design sessions with different characteristics was conducted, one considered more productive and creative, the other more pragmatic. Those segments with high entropy and those associated with high rates of change of entropy are analyzed. The creative session has a higher linkograph entropy. This result indicates the potential of using entropy to characterize a design process.
Assessing the impact of mowing on Common RedshanksTringa totanusbreeding on saltmarshes: lessons for conservation management
Authors: EXO KLAUS-MICHAEL , WELLBROCK ARNDT H. J. , SONDERMANN JULIA , MAIER MARTIN 
Published: January 2017

SummaryInformed application of habitat management measures is crucial, especially in saltmarshes that function as last refuges for breeding waders in Europe. Despite a reduction in agricultural use of saltmarshes since the establishment of the Wadden Sea National Parks at the end of the 1980s, there remains controversy regarding management measures such as the timing of mowing. We modelled the proportion of nests and chicks that would be jeopardised by mowing at different dates, using long-term breeding data of the Common RedshankTringa totanus– an endangered and widespread indicator species of saltmarshes – from four study sites in the German Wadden Sea. At two study sites in the western Jadebusen, the proportion of broods that were at risk of being killed when mowing began on 1 July ranged between 78% in early, to 96% in late, breeding years, averaging 87%. Although Common Redshanks in the eastern Jadebusen started breeding one week earlier, the model still predicted a loss of 73% of chicks; while 97% of broods were at risk on the island of Wangerooge. Postponement of mowing to 1 August reduced these proportions to 21%, 11% and 32%, respectively. This study is the first to model the positive effects of delayed mowing of saltmarshes on ground-nesting birds. By implementing adjusted mowing dates in addition to previously suggested reductions in artificial drainage, direct and indirect adverse effects caused by mowing and drainage, such as an increased predation risk, are likely to be reduced, such that a ’favourable conservation status’ according to the EC Habitats Directive may be achieved.
The Use of Emergency Lights and Sirens by Ambulances and Their Effect on Patient Outcomes and Public Safety: A Comprehensive Review of the Literature
Authors: Murray Brett , Kue Ricky 
Published: January 2017

AbstractThe benefits of emergency lights and sirens (L&S) use as warning devices by ambulances continue to be a debated topic in Emergency Medical Services (EMS). While the most widely studied aspect of L&S use has been related to their effect on ambulance response and transport times, the literature suggests minimal time savings with more questionable impact on actual patient outcomes. As L&S use has been shown to increase the risk for vehicle crashes, the secondary concern of ambulance design and safety also becomes an important aspect on potential design recommendations that could mitigate the effects of a crash on patients, EMS providers, and the general public. The least studied aspect of L&S use (and probably the most important) is their effect on patient outcomes and quality of medical care during transport. The current evidence suggests no significant improvement on patient outcomes and potential worsening to certain aspects of patient care during transport. The purpose of this review was to examine the current literature regarding ambulance L&S use and the risks they pose to EMS providers, patients, and the general public. In doing so, it will provide sound background for EMS leaders to better develop policies governing the use of L&S by ambulances and promote better research in the patient outcomes effect associated with their use. This review offers some strategies in mitigating the risks associated with L&S use, such as ways to reduce their overall use and modifying other related factors to emergency medical vehicle collisions (EMVCs).
MurrayB, KueR. The use of emergency lights and sirens by ambulances and their effect on patient outcomes and public safety: a comprehensive review of the literature. Prehosp Disaster Med. 2017;32(2):209–216.
Laser-Assisted Atom Probe Tomography of Deformed Minerals: A Zircon Case Study
Authors: La Fontaine Alexandre , Piazolo Sandra , Trimby Patrick , Yang Limei , Cairney Julie M. 
Published: January 2017

AbstractThe application of atom probe tomography to the study of minerals is a rapidly growing area. Picosecond-pulsed, ultraviolet laser (UV-355 nm) assisted atom probe tomography has been used to analyze trace element mobility within dislocations and low-angle boundaries in plastically deformed specimens of the nonconductive mineral zircon (ZrSiO4), a key material to date the earth’s geological events. Here we discuss important experimental aspects inherent in the atom probe tomography investigation of this important mineral, providing insights into the challenges in atom probe tomography characterization of minerals as a whole. We studied the influence of atom probe tomography analysis parameters on features of the mass spectra, such as the thermal tail, as well as the overall data quality. Three zircon samples with different uranium and lead content were analyzed, and particular attention was paid to ion identification in the mass spectra and detection limits of the key trace elements, lead and uranium. We also discuss the correlative use of electron backscattered diffraction in a scanning electron microscope to map the deformation in the zircon grains, and the combined use of transmission Kikuchi diffraction and focused ion beam sample preparation to assist preparation of the final atom probe tip.
A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance
Authors: Hammerling Babette C. , Najor Rita H. , Cortez Melissa Q. , Shires Sarah E. , Leon Leonardo J. , Gonzalez Eileen R. , Boassa Daniela , Phan Sébastien , Thor Andrea , Jimenez Rebecca E. , Li Hong , Kitsis Richard N. , Dorn Gerald W. , Sadoshima Junichi , Ellisman Mark H. , Gustafsson Åsa B. 
Published: January 2017

AbstractDamaged mitochondria pose a lethal threat to cells that necessitates their prompt removal. The currently recognized mechanism for disposal of mitochondria is autophagy, where damaged organelles are marked for disposal via ubiquitylation by Parkin. Here we report a novel pathway for mitochondrial elimination, in which these organelles undergo Parkin-dependent sequestration into Rab5-positive early endosomes via the ESCRT machinery. Following maturation, these endosomes deliver mitochondria to lysosomes for degradation. Although this endosomal pathway is activated by stressors that also activate mitochondrial autophagy, endosomal-mediated mitochondrial clearance is initiated before autophagy. The autophagy protein Beclin1 regulates activation of Rab5 and endosomal-mediated degradation of mitochondria, suggesting cross-talk between these two pathways. Abrogation of Rab5 function and the endosomal pathway results in the accumulation of stressed mitochondria and increases susceptibility to cell death in embryonic fibroblasts and cardiac myocytes. These data reveal a new mechanism for mitochondrial quality control mediated by Rab5 and early endosomes.
Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription
Authors: Qi Shiqian , Li Zichong , Schulze-Gahmen Ursula , Stjepanovic Goran , Zhou Qiang , Hurley James H. 
Published: January 2017

AbstractThe intrinsically disordered scaffold proteins AFF1/4 and the transcription elongation factors ELL1/2 are core components of the super elongation complex required for HIV-1 proviral transcription. Here we report the 2.0-Å resolution crystal structure of the human ELL2 C-terminal domain bound to its 50-residue binding site on AFF4, the ELLBow. The ELL2 domain has the same arch-shaped fold as the tight junction protein occludin. The ELLBow consists of an N-terminal helix followed by an extended hairpin that we refer to as the elbow joint, and occupies most of the concave surface of ELL2. This surface is important for the ability of ELL2 to promote HIV-1 Tat-mediated proviral transcription. The AFF4–ELL2 interface is imperfectly packed, leaving a cavity suggestive of a potential binding site for transcription-promoting small molecules.
KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling
Authors: Schou Kenneth B. , Mogensen Johanne B. , Morthorst Stine K. , Nielsen Brian S. , Aleliunaite Aiste , Serra-Marques Andrea , Fürstenberg Nicoline , Saunier Sophie , Bizet Albane A. , Veland Iben R. , Akhmanova Anna , Christensen Søren T. , Pedersen Lotte B. 
Published: January 2017

AbstractCiliary membrane composition is controlled by transition zone (TZ) proteins such as RPGRIP1, RPGRIPL and NPHP4, which are vital for balanced coordination of diverse signalling systems like the Sonic hedgehog (Shh) pathway. Activation of this pathway involves Shh-induced ciliary accumulation of Smoothened (SMO), which is disrupted by disease-causing mutations in TZ components. Here we identify kinesin-3 motor protein KIF13B as a novel member of the RPGRIP1N-C2 domain-containing protein family and show that KIF13B regulates TZ membrane composition and ciliary SMO accumulation. KIF13B is upregulated during ciliogenesis and is recruited to the ciliary base by NPHP4, which binds to two distinct sites in the KIF13B tail region, including an RPGRIP1N-C2 domain. KIF13B and NPHP4 are both essential for establishment of a CAV1 membrane microdomain at the TZ, which in turn is required for Shh-induced ciliary SMO accumulation. Thus KIF13B is a novel regulator of ciliary TZ configuration, membrane composition and Shh signalling.
Single-step ambient-air synthesis of graphene from renewable precursors as electrochemical genosensor
Authors: Seo Dong Han , Pineda Shafique , Fang Jinghua , Gozukara Yesim , Yick Samuel , Bendavid Avi , Lam Simon Kwai Hung , Murdock Adrian T. , Murphy Anthony B. , Han Zhao Jun , Ostrikov Kostya 
Published: January 2017

AbstractThermal chemical vapour deposition techniques for graphene fabrication, while promising, are thus far limited by resource-consuming and energy-intensive principles. In particular, purified gases and extensive vacuum processing are necessary for creating a highly controlled environment, isolated from ambient air, to enable the growth of graphene films. Here we exploit the ambient-air environment to enable the growth of graphene films, without the need for compressed gases. A renewable natural precursor, soybean oil, is transformed into continuous graphene films, composed of single-to-few layers, in a single step. The enabling parameters for controlled synthesis and tailored properties of the graphene film are discussed, and a mechanism for the ambient-air growth is proposed. Furthermore, the functionality of the graphene is demonstrated through direct utilization as an electrode to realize an effective electrochemical genosensor. Our method is applicable to other types of renewable precursors and may open a new avenue for low-cost synthesis of graphene films.
The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein
Authors: Miyakawa Kei , Nishi Mayuko , Matsunaga Satoko , Okayama Akiko , Anraku Masaki , Kudoh Ayumi , Hirano Hisashi , Kimura Hirokazu , Morikawa Yuko , Yamamoto Naoki , Ono Akira , Ryo Akihide 
Published: January 2017

AbstractDiverse cellular proteins and RNAs are tightly regulated in their subcellular localization to exert their local function. Here we report that the tumour suppressor adenomatous polyposis coli protein (APC) directs the localization and assembly of human immunodeficiency virus (HIV)-1 Gag polyprotein at distinct membrane components to enable the efficient production and spread of infectious viral particles. A proteomic analysis and subsequent biomolecular interaction assay reveals that the carboxyl terminus of APC interacts with the matrix region of Gag. Ectopic expression of APC, but not its familial adenomatous polyposis-related truncation mutant, prominently enhances HIV-1 production. Conversely, the depletion of APC leads to a significant decrease in membrane targeting of viral components, resulting in the severe loss of production of infectious virions. Furthermore, APC promotes the directional assembly of viral components at virological synapses, thereby facilitating cell-to-cell viral transmission. These findings reveal an unexpected role of APC in the directional spread of HIV-1.
Preserving π-conjugation in covalently functionalized carbon nanotubes for optoelectronic applications
Authors: Setaro Antonio , Adeli Mohsen , Glaeske Mareen , Przyrembel Daniel , Bisswanger Timo , Gordeev Georgy , Maschietto Federica , Faghani Abbas , Paulus Beate , Weinelt Martin , Arenal Raul , Haag Rainer , Reich Stephanie 
Published: January 2017

AbstractCovalent functionalization tailors carbon nanotubes for a wide range of applications in varying environments. Its strength and stability of attachment come at the price of degrading the carbon nanotubes sp2 network and destroying the tubes electronic and optoelectronic features. Here we present a non-destructive, covalent, gram-scale functionalization of single-walled carbon nanotubes by a new [2+1] cycloaddition. The reaction rebuilds the extended π-network, thereby retaining the outstanding quantum optoelectronic properties of carbon nanotubes, including bright light emission at high degree of functionalization (1 group per 25 carbon atoms). The conjugation method described here opens the way for advanced tailoring nanotubes as demonstrated for light-triggered reversible doping through photochromic molecular switches and nanoplasmonic gold-nanotube hybrids with enhanced infrared light emission.
Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes
Authors: Liu Xianpeng , Zhao Bo , Sun Limin , Bhuripanyo Karan , Wang Yiyang , Bi Yingtao , Davuluri Ramana V. , Duong Duc M. , Nanavati Dhaval , Yin Jun , Kiyokawa Hiroaki 
Published: January 2017

AbstractProtein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2 and ubiquitin ligase E3. Uba1 was thought to be the only E1 until the recent identification of Uba6. To differentiate the biological functions of Uba1 and Uba6, we applied an orthogonal ubiquitin transfer (OUT) technology to profile their ubiquitination targets in mammalian cells. By expressing pairs of an engineered ubiquitin and engineered Uba1 or Uba6 that were generated for exclusive interactions, we identified 697 potential Uba6 targets and 527 potential Uba1 targets with 258 overlaps. Bioinformatics analysis reveals substantial differences in pathways involving Uba1- and Uba6-specific targets. We demonstrate that polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1, and that Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. These data suggest that distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6.
THZ1 targeting CDK7 suppresses STAT transcriptional activity and sensitizes T-cell lymphomas to BCL2 inhibitors
Authors: Cayrol Florencia , Praditsuktavorn Pannee , Fernando Tharu M. , Kwiatkowski Nicholas , Marullo Rosella , Calvo-Vidal M. Nieves , Phillip Jude , Pera Benet , Yang Shao Ning , Takpradit Kaipol , Roman Lidia , Gaudiano Marcello , Crescenzo Ramona , Ruan Jia , Inghirami Giorgio , Zhang Tinghu , Cremaschi Graciela , Gray Nathanael S. , Cerchietti Leandro 
Published: January 2017

AbstractPeripheral T-cell lymphomas (PTCL) are aggressive diseases with poor response to chemotherapy and dismal survival. Identification of effective strategies to target PTCL biology represents an urgent need. Here we report that PTCL are sensitive to transcription-targeting drugs, and, in particular, to THZ1, a covalent inhibitor of cyclin-dependent kinase 7 (CDK7). The STAT-signalling pathway is highly vulnerable to THZ1 even in PTCL cells that carry the activating STAT3 mutation Y640F. In mutant cells, CDK7 inhibition decreases STAT3 chromatin binding and expression of highly transcribed target genes like MYC, PIM1, MCL1, CD30, IL2RA, CDC25A and IL4R. In surviving cells, THZ1 decreases the expression of STAT-regulated anti-apoptotic BH3 family members MCL1 and BCL-XL sensitizing PTCL cells to BH3 mimetic drugs. Accordingly, the combination of THZ1 and the BH3 mimetic obatoclax improves lymphoma growth control in a primary PTCL ex vivo culture and in two STAT3-mutant PTCL xenografts, delineating a potential targeted agent-based therapeutic option for these patients.