bluuebunny/crossref_metadata_2025_split huggingface dataset:
Schlafqualität und deren Beziehung zu attentional-exekutiven Funktionsleistungen bei unipolar depressiven Patienten
Authors: Kundermann Bernd , Fockenberg Stanislava , Cabanel Nicole , Müller Matthias J. 
Published: January 2017

Zusammenfassung. Die Beziehung zwischen kognitiven Defiziten und Schlafstörungen depressiver Patienten wurde bisher wenig untersucht. Stationär behandelte depressive Patienten beantworteten Fragebögen zur Depressivität und Schlafqualität (Pittsburgh Sleep Quality Inventory, PSQI), gefolgt von neuropsychologischen Untersuchungen zu attentional-exekutiven Funktionsleistungen (Trail Making Test: TMT-A, TMT-B) an Tag 1 (abends) und Tag 2 (morgens). Patienten mit schweren Schlafstörungen (PSQI > 10, n = 8) erbrachten gegenüber Patienten mit maximal moderat ausgeprägten Schlafstörungen (PSQI ≤ 10, n = 8) signifikant geringere Leistungen im Rahmen des TMT-A und TMT-B. Signifikante positive Korrelationen zwischen dem Globalwert des PSQI und der TMT-B-Bearbeitungszeit blieben auch unter statistischer Berücksichtigung von Kovariaten erhalten. Diese Ergebnisse unterstreichen die enge Beziehung zwischen kognitiven Dysfunktionen und Schlafstörungen bei depressiven Patienten. Mögliche therapeutische Implikationen werden diskutiert.
[Au]/[Ag]-catalysed expedient synthesis of branched heneicosafuranosyl arabinogalactan motif of Mycobacterium tuberculosis cell wall
Authors: Thadke Shivaji A. , Mishra Bijoyananda , Islam Maidul , Pasari Sandip , Manmode Sujit , Rao Boddu Venkateswara , Neralkar Mahesh , Shinde Ganesh P. , Walke Gulab , Hotha Srinivas 
Published: January 2017

AbstractEmergence of multidrug-resistant and extreme-drug-resistant strains of Mycobacterium tuberculosis (MTb) can cause serious socioeconomic burdens. Arabinogalactan present on the cellular envelope of MTb is unique and is required for its survival; access to arabinogalactan is essential for understanding the biosynthetic machinery that assembles it. Isolation from Nature is a herculean task and, as a result, chemical synthesis is the most sought after technique. Here we report a convergent synthesis of branched heneicosafuranosyl arabinogalactan (HAG) of MTb. Key furanosylations are performed using [Au]/[Ag] catalysts. The synthesis of HAG is achieved by the repetitive use of three reactions namely 1,2-trans furanoside synthesis by propargyl 1,2-orthoester donors, unmasking of silyl ether, and conversion of n-pentenyl furanosides into 1,2-orthoesters. Synthesis of HAG is achieved in 47 steps (with an overall yield of 0.09%) of which 21 are installation of furanosidic linkages in a stereoselective manner.
Deep eutectic-solvothermal synthesis of nanostructured ceria
Authors: Hammond Oliver S. , Edler Karen J. , Bowron Daniel T. , Torrente-Murciano Laura 
Published: January 2017

AbstractCeria is a technologically important material with applications in catalysis, emissions control and solid-oxide fuel cells. Nanostructured ceria becomes profoundly more active due to its enhanced surface area to volume ratio, reactive surface oxygen vacancy concentration and superior oxygen storage capacity. Here we report the synthesis of nanostructured ceria using the green Deep Eutectic Solvent reline, which allows morphology and porosity control in one of the less energy-intensive routes reported to date. Using wide Q-range liquid-phase neutron diffraction, we elucidate the mechanism of reaction at a molecular scale at considerably milder conditions than the conventional hydrothermal synthetic routes. The reline solvent plays the role of a latent supramolecular catalyst where the increase in reaction rate from solvent-driven pre-organization of the reactants is most significant. This fundamental understanding of deep eutectic-solvothermal methodology will enable future developments in low-temperature synthesis of nanostructured ceria, facilitating its large-scale manufacturing using green, economic, non-toxic solvents.
A polychromatic ‘greenbeard’ locus determines patterns of cooperation in a social amoeba
Authors: Gruenheit Nicole , Parkinson Katie , Stewart Balint , Howie Jennifer A. , Wolf Jason B. , Thompson Christopher R. L. 
Published: January 2017

AbstractCheaters disrupt cooperation by reaping the benefits without paying their fair share of associated costs. Cheater impact can be diminished if cooperators display a tag (‘greenbeard’) and recognise and preferentially direct cooperation towards other tag carriers. Despite its popular appeal, the feasibility of such greenbeards has been questioned because the complex patterns of partner-specific cooperative behaviours seen in nature require greenbeards to come in different colours. Here we show that a locus (‘Tgr’) of a social amoeba represents a polychromatic greenbeard. Patterns of natural Tgr locus sequence polymorphisms predict partner-specific patterns of cooperation by underlying variation in partner-specific protein–protein binding strength and recognition specificity. Finally, Tgr locus polymorphisms increase fitness because they help avoid potential costs of cooperating with incompatible partners. These results suggest that a polychromatic greenbeard can provide a key mechanism for the evolutionary maintenance of cooperation.
Identical folds used for distinct mechanical functions of the bacterial flagellar rod and hook
Authors: Fujii Takashi , Kato Takayuki , Hiraoka Koichi D. , Miyata Tomoko , Minamino Tohru , Chevance Fabienne F. V. , Hughes Kelly T. , Namba Keiichi 
Published: January 2017

AbstractThe bacterial flagellum is a motile organelle driven by a rotary motor, and its axial portions function as a drive shaft (rod), a universal joint (hook) and a helical propeller (filament). The rod and hook are directly connected to each other, with their subunit proteins FlgG and FlgE having 39% sequence identity, but show distinct mechanical properties; the rod is straight and rigid as a drive shaft whereas the hook is flexible in bending as a universal joint. Here we report the structure of the rod and comparison with that of the hook. While these two structures have the same helical symmetry and repeat distance and nearly identical folds of corresponding domains, the domain orientations differ by ∼7°, resulting in tight and loose axial subunit packing in the rod and hook, respectively, conferring the rigidity on the rod and flexibility on the hook. This provides a good example of versatile use of a protein structure in biological organisms.
Tricolor R/G/B Laser Diode Based Eye-Safe White Lighting Communication Beyond 8 Gbit/s
Authors: Wu Tsai-Chen , Chi Yu-Chieh , Wang Huai-Yung , Tsai Cheng-Ting , Huang Yu-Fang , Lin Gong-Ru 
Published: January 2017

AbstractWhite light generation by mixing red, green, and blue laser diodes (RGB LDs) was demonstrated with Commission International de l’Eclairage coordinates of (0.2928, 0.2981), a correlated color temperature of 8382 K, and a color rendering index of 54.4 to provide a maximal illuminance of 7540 lux. All the white lights generated using RGB LDs were set within the risk group-1 criterion to avoid the blue-light hazard to human eyes. In addition, the RGB-LD mixed white light was diffused using a frosted glass to avoid optical aberration and to improve the performance of the lighting source. In addition, visible light communication (VLC) by using RGB-LD mixed white-light carriers and a point-to-point scheme over 1 m was performed in the directly modulated 16-QAM OFDM data format. In back-to-back transmission, the maximal allowable data rate at 10.8, 10.4, and 8 Gbps was determined for R, G, and B LDs, respectively. Moreover, the RGB-LD mixed white light-based indoor wavelength-division multiplexing (WDM)-VLC system yielded a total allowable transmission data rate of 8.8 Gbps over 0.5 m in free space. Such a high-speed RGB-LD mixed WDM-VLC system without any channel interference can be used to simultaneously provide data transmission and white lighting in an indoor environment.
Soft Nanocomposite Based Multi-point, Multi-directional Strain Mapping Sensor Using Anisotropic Electrical Impedance Tomography
Authors: Lee Hyosang , Kwon Donguk , Cho Haedo , Park Inkyu , Kim Jung 
Published: January 2017

AbstractThe practical utilization of soft nanocomposites as a strain mapping sensor in tactile sensors and artificial skins requires robustness for various contact conditions as well as low-cost fabrication process for large three dimensional surfaces. In this work, we propose a multi-point and multi-directional strain mapping sensor based on multiwall carbon nanotube (MWCNT)-silicone elastomer nanocomposites and anisotropic electrical impedance tomography (aEIT). Based on the anisotropic resistivity of the sensor, aEIT technique can reconstruct anisotropic resistivity distributions using electrodes around the sensor boundary. This strain mapping sensor successfully estimated stretch displacements (error of 0.54 ± 0.53 mm), surface normal forces (error of 0.61 ± 0.62 N), and multi-point contact locations (error of 1.88 ± 0.95 mm in 30 mm × 30 mm area for a planar shaped sensor and error of 4.80 ± 3.05 mm in 40 mm × 110 mm area for a three dimensional contoured sensor). In addition, the direction of lateral stretch was also identified by reconstructing anisotropic distributions of electrical resistivity. Finally, a soft human-machine interface device was demonstrated as a practical application of the developed sensor.
High Quality Bioreplication of Intricate Nanostructures from a Fragile Gecko Skin Surface with Bactericidal Properties
Authors: Green David William , Lee Kenneth Ka-Ho , Watson Jolanta Anna , Kim Hyun-Yi , Yoon Kyung-Sik , Kim Eun-Jung , Lee Jong-Min , Watson Gregory Shaun , Jung Han-Sung 
Published: January 2017

AbstractThe external epithelial surfaces of plants and animals are frequently carpeted with small micro- and nanostructures, which broadens their adaptive capabilities in challenging physical habitats. Hairs and other shaped protuberances manage with excessive water, light contaminants, predators or parasites in innovative ways. We are interested in transferring these intricate architectures onto biomedical devices and daily-life surfaces. Such a project requires a very rapid and accurate small-scale fabrication process not involving lithography. In this study, we describe a simple benchtop biotemplating method using shed gecko lizard skin that generates duplicates that closely replicate the small nanotipped hairs (spinules) that cover the original skin. Synthetic replication of the spinule arrays in popular biomaterials closely matched the natural spinules in length. More significantly, the shape, curvature and nanotips of the synthetic arrays are virtually identical to the natural ones. Despite some small differences, the synthetic gecko skin surface resisted wetting and bacterial contamination at the same level as natural shed skin templates. Such synthetic gecko skin surfaces are excellent platforms to test for bacterial control in clinical settings. We envision testing the biocidal properties of the well-matched templates for fungal spores and viral resistance in biomedicine as well as co/multi-cultures.
New determinants of olfactory habituation
Authors: Sinding Charlotte , Valadier François , Al-Hassani Viviana , Feron Gilles , Tromelin Anne , Kontaris Ioannis , Hummel Thomas 
Published: January 2017

AbstractHabituation is a filter that optimizes the processing of information by our brain in all sensory modalities. It results in an unconscious reduced responsiveness to continuous or repetitive stimulation. In olfaction, the main question is whether habituation works the same way for any odorant or whether we habituate differently to each odorant? In particular, whether chemical, physical or perceptual cues can limit or increase habituation. To test this, the odour intensity of 32 odorants differing in physicochemical characteristics was rated by 58 participants continuously during 120s. Each odorant was delivered at a constant concentration. Results showed odorants differed significantly in habituation, highlighting the multifactoriality of habituation. Additionally habituation was predicted from 15 physico-chemical and perceptual characteristics of the odorants. The analysis highlighted the importance of trigeminality which is highly correlated to intensity and pleasantness. The vapour pressure, the molecular weight, the Odor Activity Value (OAV) and the number of double bonds mostly contributed to the modulation of habituation. Moreover, length of the carbon chain, number of conformers and hydrophobicity contributed to a lesser extent to the modulation of habituation. These results highlight new principles involved in the fundamental process of habituation, notably trigeminality and the physicochemical characteristics associated.
IL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
Authors: Shen Jieliang , Xu Shengxi , Zhou Hao , Liu Huzhe , Jiang Wei , Hao Jie , Hu Zhenming 
Published: January 2017

AbstractIL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cytochrome C released from mitochondria to cytosol, which proved mitochondria-meidated apoptosis was induced. Subsequently, mitochondria damage was detected under IL-1β stimualtion. In addition, IL-1β-mediated injuried mitochondria contributes to activate autophagy. However, pretreatment with the autophagy inhibitor 3-methyladenine showed the potential in further elevating the apoptosis rate induced by IL-1β in NP cells. Our results indicated that the mitochondrial pathway was involved in IL-1β-induced apoptosis of NP cells. Meanwhile, the damaged mitochondria-induced autophagy played a protective role against apoptosis, suggesting a postive feedback mechanism under inflammatory stress.
Kernel-based Joint Feature Selection and Max-Margin Classification for Early Diagnosis of Parkinson’s Disease
Authors: Adeli Ehsan , Wu Guorong , Saghafi Behrouz , An Le , Shi Feng , Shen Dinggang 
Published: January 2017

AbstractFeature selection methods usually select the most compact and relevant set of features based on their contribution to a linear regression model. Thus, these features might not be the best for a non-linear classifier. This is especially crucial for the tasks, in which the performance is heavily dependent on the feature selection techniques, like the diagnosis of neurodegenerative diseases. Parkinson’s disease (PD) is one of the most common neurodegenerative disorders, which progresses slowly while affects the quality of life dramatically. In this paper, we use the data acquired from multi-modal neuroimaging data to diagnose PD by investigating the brain regions, known to be affected at the early stages. We propose a joint kernel-based feature selection and classification framework. Unlike conventional feature selection techniques that select features based on their performance in the original input feature space, we select features that best benefit the classification scheme in the kernel space. We further propose kernel functions, specifically designed for our non-negative feature types. We use MRI and SPECT data of 538 subjects from the PPMI database, and obtain a diagnosis accuracy of 97.5%, which outperforms all baseline and state-of-the-art methods.
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Authors: Haft Daniel H. , Pierce Phillip G. , Mayclin Stephen J. , Sullivan Amy , Gardberg Anna S. , Abendroth Jan , Begley Darren W. , Phan Isabelle Q. , Staker Bart L. , Myler Peter J. , Marathias Vasilios M. , Lorimer Donald D. , Edwards Thomas E. 
Published: January 2017

AbstractDuring human infection, Mycobacterium tuberculosis (Mtb) survives the normally bacteriocidal phagosome of macrophages. Mtb and related species may be able to combat this harsh acidic environment which contains reactive oxygen species due to the mycobacterial genomes encoding a large number of dehydrogenases. Typically, dehydrogenase cofactor binding sites are open to solvent, which allows NAD/NADH exchange to support multiple turnover. Interestingly, mycobacterial short chain dehydrogenases/reductases (SDRs) within family TIGR03971 contain an insertion at the NAD binding site. Here we present crystal structures of 9 mycobacterial SDRs in which the insertion buries the NAD cofactor except for a small portion of the nicotinamide ring. Line broadening and STD-NMR experiments did not show NAD or NADH exchange on the NMR timescale. STD-NMR demonstrated binding of the potential substrate carveol, the potential product carvone, the inhibitor tricyclazol, and an external redox partner 2,6-dichloroindophenol (DCIP). Therefore, these SDRs appear to contain a non-exchangeable NAD cofactor and may rely on an external redox partner, rather than cofactor exchange, for multiple turnover. Incidentally, these genes always appear in conjunction with the mftA gene, which encodes the short peptide MftA, and with other genes proposed to convert MftA into the external redox partner mycofactocin.
Genome-wide association study identifies four SNPs associated with response to platinum-based neoadjuvant chemotherapy for cervical cancer
Authors: Li Xiong , Huang Kecheng , Zhang Qinghua , Zhou Jin , Sun Haiying , Tang Fangxu , Zhou Hang , Hu Ting , Wang Shaoshuai , Jia Yao , Yang Ru , Chen Yile , Cheng Xiaodong , Lv Weiguo , Wu Li , Xing Hui , Wang Lin , Zhou Shasha , Yao Yuan , Wang Xiaoli , Suolang Quzhen , Shen Jian , Xi Ling , Hu Junbo , Wang Hui , Chen Gang , Gao Qinglei , Xie Xing , Wang Shixuan , Li Shuang , Ma Ding 
Published: January 2017

AbstractTo identify genomic markers associated with the response to neoadjuvant chemotherapy (NACT) in patients with cervical cancer, we performed a three-stage genome-wide association study (GWAS) in the Han Chinese population. A total of 596 patients with stage IA2-IIIB cervical cancer were enrolled in this study. One single nucleotide polymorphism (SNP) (rs6812281, per allele OR = 2.37, P = 9.0 × 10−9) located at 4q34.3 reached GWAS significance (P < 5.0 × 10−8). Another three SNPs, rs4590782 (10q26.2, P = 1.59 × 10−5, per allele OR = 0.48), rs1742101 (14q32.11, P = 7.11 × 10−6, per allele OR = 0.52), and rs1364121 (16q23.3, P = 3.15 × 10−6, per allele OR = 1.98), exhibited strong evidence of associations with response to neoadjuvant chemotherapy. Patients with a C allele (CT + CC) of rs4590782 had better 5-year overall survival rates (82.9% vs. 75.8%, P = 0.083) and 5-year disease-free survival rate (80.8% vs. 72.7%, P = 0.021) than those without a C allele. Our findings help to characterize the genetic etiology of the response to neoadjuvant chemotherapy in patients with cervical cancer.
Physical Activity, TV Watching Time, Sleeping, and Risk of Obesity and Hyperglycemia in the Offspring of Mothers with Gestational Diabetes Mellitus
Authors: Zhang Tao , Wang Peng , Liu Huikun , Wang Leishen , Li Weiqin , Leng Junhong , Li Nan , Zhang Shuang , Qi Lu , Tuomilehto Jaakko , Yu Zhijie , Yang Xilin , Hu Gang 
Published: January 2017

AbstractWe investigated the association of physical activity, TV watching time, sleeping time with the risks of obesity and hyperglycemia among 1263 offspring aged 1–5 years of mothers with gestational diabetes (GDM) in a cross-sectional study. Logistic regression models were used to obtain the odd ratios (ORs) (95% confidence intervals [CI]) of childhood obesity and hyperglycemia associated with different levels of indoor activity, outdoor activity, TV watching, and sleeping time. The multivariable-adjusted ORs of obesity based on different levels of TV watching time (0, <1.0, and ≥1.0 hour/day) were 1.00, 1.21 (95% CI 0.72–2.05), and 2.20 (95% CI 1.33–3.63) (Ptrend = 0.003), respectively. The multivariable-adjusted ORs of hyperglycemia based on different levels of indoor activity (<5.0, 5.0–6.9, and ≥7.0 hours/day) were 1.00, 0.74 (95% CI 0.45–1.21), and 0.49 (95% CI 0.28–0.84) (Ptrend = 0.034), respectively. The multivariable-adjusted ORs of hyperglycemia associated with different levels of sleeping time (<11.0, 11.0–11.9, and ≥12.0 hours/day) were 1.00, 0.67 (95% CI 0.42–1.05), and 0.39 (95% CI 0.23–0.67) (Ptrend = 0.003), respectively. The present study indicated a positive association of TV watching with the risk of obesity, and an inverse association of either indoor activity or sleeping time with the risk of hyperglycemia among offspring born to GDM mothers in Tianjin, China.
Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.)
Authors: Mori Kazuki , Shirasawa Kenta , Nogata Hitoshi , Hirata Chiharu , Tashiro Kosuke , Habu Tsuyoshi , Kim Sangwan , Himeno Shuichi , Kuhara Satoru , Ikegami Hidetoshi 
Published: January 2017

AbstractWith the aim of identifying sex determinants of fig, we generated the first draft genome sequence of fig and conducted the subsequent analyses. Linkage analysis with a high-density genetic map established by a restriction-site associated sequencing technique, and genome-wide association study followed by whole-genome resequencing analysis identified two missense mutations in RESPONSIVE-TO-ANTAGONIST1 (RAN1) orthologue encoding copper-transporting ATPase completely associated with sex phenotypes of investigated figs. This result suggests that RAN1 is a possible sex determinant candidate in the fig genome. The genomic resources and genetic findings obtained in this study can contribute to general understanding of Ficus species and provide an insight into fig’s and plant’s sex determination system.
Parathyroid Hormone-Like Hormone is a Poor Prognosis Marker of Head and Neck Cancer and Promotes Cell Growth via RUNX2 Regulation
Authors: Chang Wei-Min , Lin Yuan-Feng , Su Chia-Yi , Peng Hsuan-Yu , Chang Yu-Chan , Hsiao Jenn-Ren , Chen Chi-Long , Chang Jang-Yang , Shieh Yi-Shing , Hsiao Michael , Shiah Shine-Gwo 
Published: January 2017

AbstractParathyroid Hormone-Like Hormone (PTHLH) is an autocrine/paracrine ligand that is up-regulated in head and neck squamous cell carcinoma (HNSCC). However, the cellular function and regulatory mechanism in HNSCC remains obscure. We investigated the clinical significance of PTHLH in HNSCC patients, and verified the role of RUNX2/PTHLH axis, which is stimulated HNSCC cell growth. In patients, PTHLH is a poor prognosis marker. PTHLH expression lead to increasing the cell proliferation potential through an autocrine/paracrine role and elevating blood calcium level in Nod-SCID mice. In public HNSCC microarray cohorts, PTHLH is found to be co-expressed with RUNX2. Physiologically, PTHLH is regulated by RUNX2 and also acting as key calcium regulator. However, elevations of calcium concentration also increased the RUNX2 expression. PTHLH, calcium, and RUNX2 form a positive feedback loop in HNSCC. Furthermore, ectopic RUNX2 expression also increased PTHLH expression and promoted proliferation potential through PTHLH expression. Using cDNA microarray analysis, we found PTHLH also stimulated expression of cell cycle regulators, namely CCNA2, CCNE2, and CDC25A in HNSCC cells, and these genes are also up-regulated in HNSCC patients. In summary, our results reveal that PTHLH expression is a poor prognosis marker in HNSCC patients, and RUNX2-PTHLH axis contributes to HNSCC tumor growth.