bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light controls cerebral blood flow in naive animals
Authors: Rungta Ravi L , Osmanski Bruno-Félix , Boido Davide , Tanter Mickael , Charpak Serge 
Published: January 2017

AbstractOptogenetics is increasingly used to map brain activation using techniques that rely on functional hyperaemia, such as opto-fMRI. Here we test whether light stimulation protocols similar to those commonly used in opto-fMRI or to study neurovascular coupling modulate blood flow in mice that do not express light sensitive proteins. Combining two-photon laser scanning microscopy and ultrafast functional ultrasound imaging, we report that in the naive mouse brain, light per se causes a calcium decrease in arteriolar smooth muscle cells, leading to pronounced vasodilation, without excitation of neurons and astrocytes. This photodilation is reversible, reproducible and energy-dependent, appearing at about 0.5 mJ. These results impose careful consideration on the use of photo-activation in studies involving blood flow regulation, as well as in studies requiring prolonged and repetitive stimulations to correct cellular defects in pathological models. They also suggest that light could be used to locally increase blood flow in a controlled fashion.
SC83288 is a clinical development candidate for the treatment of severe malaria
Authors: Pegoraro Stefano , Duffey Maëlle , Otto Thomas D , Wang Yulin , Rösemann Roman , Baumgartner Roland , Fehler Stefanie K , Lucantoni Leonardo , Avery Vicky M , Moreno-Sabater Alicia , Mazier Dominique , Vial Henri J , Strobl Stefan , Sanchez Cecilia P , Lanzer Michael 
Published: January 2017

AbstractSevere malaria is a life-threatening complication of an infection with the protozoan parasite Plasmodium falciparum, which requires immediate treatment. Safety and efficacy concerns with currently used drugs accentuate the need for new chemotherapeutic options against severe malaria. Here we describe a medicinal chemistry program starting from amicarbalide that led to two compounds with optimized pharmacological and antiparasitic properties. SC81458 and the clinical development candidate, SC83288, are fast-acting compounds that can cure a P. falciparum infection in a humanized NOD/SCID mouse model system. Detailed preclinical pharmacokinetic and toxicological studies reveal no observable drawbacks. Ultra-deep sequencing of resistant parasites identifies the sarco/endoplasmic reticulum Ca2+ transporting PfATP6 as a putative determinant of resistance to SC81458 and SC83288. Features, such as fast parasite killing, good safety margin, a potentially novel mode of action and a distinct chemotype support the clinical development of SC83288, as an intravenous application for the treatment of severe malaria.
Climate change reduces extent of temperate drylands and intensifies drought in deep soils
Authors: Schlaepfer Daniel R. , Bradford John B. , Lauenroth William K. , Munson Seth M. , Tietjen Britta , Hall Sonia A. , Wilson Scott D. , Duniway Michael C. , Jia Gensuo , Pyke David A. , Lkhagva Ariuntsetseg , Jamiyansharav Khishigbayar 
Published: January 2017

AbstractDrylands cover 40% of the global terrestrial surface and provide important ecosystem services. While drylands as a whole are expected to increase in extent and aridity in coming decades, temperature and precipitation forecasts vary by latitude and geographic region suggesting different trajectories for tropical, subtropical, and temperate drylands. Uncertainty in the future of tropical and subtropical drylands is well constrained, whereas soil moisture and ecological droughts, which drive vegetation productivity and composition, remain poorly understood in temperate drylands. Here we show that, over the twenty first century, temperate drylands may contract by a third, primarily converting to subtropical drylands, and that deep soil layers could be increasingly dry during the growing season. These changes imply major shifts in vegetation and ecosystem service delivery. Our results illustrate the importance of appropriate drought measures and, as a global study that focuses on temperate drylands, highlight a distinct fate for these highly populated areas.
Experimental evidence for circular inference in schizophrenia
Authors: Jardri Renaud , Duverne Sandrine , Litvinova Alexandra S , Denève Sophie 
Published: January 2017

AbstractSchizophrenia (SCZ) is a complex mental disorder that may result in some combination of hallucinations, delusions and disorganized thinking. Here SCZ patients and healthy controls (CTLs) report their level of confidence on a forced-choice task that manipulated the strength of sensory evidence and prior information. Neither group’s responses can be explained by simple Bayesian inference. Rather, individual responses are best captured by a model with different degrees of circular inference. Circular inference refers to a corruption of sensory data by prior information and vice versa, leading us to ‘see what we expect’ (through descending loops), to ‘expect what we see’ (through ascending loops) or both. Ascending loops are stronger for SCZ than CTLs and correlate with the severity of positive symptoms. Descending loops correlate with the severity of negative symptoms. Both loops correlate with disorganized symptoms. The findings suggest that circular inference might mediate the clinical manifestations of SCZ.
Articulation points in complex networks
Authors: Tian Liang , Bashan Amir , Shi Da-Ning , Liu Yang-Yu 
Published: January 2017

AbstractAn articulation point in a network is a node whose removal disconnects the network. Those nodes play key roles in ensuring connectivity of many real-world networks, from infrastructure networks to protein interaction networks and terrorist communication networks. Despite their fundamental importance, a general framework of studying articulation points in complex networks is lacking. Here we develop analytical tools to study key issues pertinent to articulation points, such as the expected number of them and the network vulnerability against their removal, in an arbitrary complex network. We find that a greedy articulation point removal process provides us a different perspective on the organizational principles of complex networks. Moreover, this process results in a rich phase diagram with two fundamentally different types of percolation transitions. Our results shed light on the design of more resilient infrastructure networks and the effective destruction of terrorist communication networks.
Convergent recombination suppression suggests role of sexual selection in guppy sex chromosome formation
Authors: Wright Alison E. , Darolti Iulia , Bloch Natasha I. , Oostra Vicencio , Sandkam Ben , Buechel Severine D. , Kolm Niclas , Breden Felix , Vicoso Beatriz , Mank Judith E. 
Published: January 2017

AbstractSex chromosomes evolve once recombination is halted between a homologous pair of chromosomes. The dominant model of sex chromosome evolution posits that recombination is suppressed between emerging X and Y chromosomes in order to resolve sexual conflict. Here we test this model using whole genome and transcriptome resequencing data in the guppy, a model for sexual selection with many Y-linked colour traits. We show that although the nascent Y chromosome encompasses nearly half of the linkage group, there has been no perceptible degradation of Y chromosome gene content or activity. Using replicate wild populations with differing levels of sexually antagonistic selection for colour, we also show that sexual selection leads to greater expansion of the non-recombining region and increased Y chromosome divergence. These results provide empirical support for longstanding models of sex chromosome catalysis, and suggest an important role for sexual selection and sexual conflict in genome evolution.
Deferasirox-induced iron depletion promotes BclxL downregulation and death of proximal tubular cells
Authors: Martin-Sanchez Diego , Gallegos-Villalobos Angel , Fontecha-Barriuso Miguel , Carrasco Susana , Sanchez-Niño Maria Dolores , Lopez-Hernandez Francisco J , Ruiz-Ortega Marta , Egido Jesus , Ortiz Alberto , Sanz Ana Belén 
Published: January 2017

AbstractIron deficiency has been associated with kidney injury. Deferasirox is an oral iron chelator used to treat blood transfusion-related iron overload. Nephrotoxicity is the most serious and common adverse effect of deferasirox and may present as an acute or chronic kidney disease. However, scarce data are available on the molecular mechanisms of nephrotoxicity. We explored the therapeutic modulation of deferasirox-induced proximal tubular cell death in culture. Deferasirox induced dose-dependent tubular cell death and AnexxinV/7AAD staining showed features of apoptosis and necrosis. However, despite inhibiting caspase-3 activation, the pan-caspase inhibitor zVAD-fmk failed to prevent deferasirox-induced cell death. Moreover, zVAD increased deferasirox-induced cell death, a feature sometimes found in necroptosis. Electron microscopy identified mitochondrial injury and features of necrosis. However, neither necrostatin-1 nor RIP3 knockdown prevented deferasirox-induced cell death. Deferasirox caused BclxL depletion and BclxL overexpression was protective. Preventing iron depletion protected from BclxL downregulation and deferasirox cytotoxicity. In conclusion, deferasirox promoted iron depletion-dependent cell death characterized by BclxL downregulation. BclxL overexpression was protective, suggesting a role for BclxL downregulation in iron depletion-induced cell death. This information may be used to develop novel nephroprotective strategies. Furthermore, it supports the concept that monitoring kidney tissue iron depletion may decrease the risk of deferasirox nephrotoxicity.
A portable luminescent thermometer based on green up-conversion emission of Er3+/Yb3+ co-doped tellurite glass
Authors: Manzani Danilo , Petruci João Flávio da Silveira , Nigoghossian Karina , Cardoso Arnaldo Alves , Ribeiro Sidney J. L. 
Published: January 2017

AbstractThe determination of temperature is essential in many applications in the biomedical, technological, and industrial fields. Optical thermometry appears to be an excellent alternative for conventional electric temperature sensors because it is a non-contact method that offers a fast response, electromagnetic passivity, and high temperature sensitivity. In this paper, we propose an optical thermometer probe comprising an Er3+/Yb3+ co-doped tellurite glass attached to the tip of an optical fibre and optically coupled to a laser source and a portable USB spectrometer. The ratio of the up-conversion green emission integrated peak areas when excited at 980 nm was temperature dependent, and it was used to calibrate the thermometer. The thermometer was operated in the range of 5–50 °C and 50–200 °C, and it revealed excellent linearity (r2 > 0.99), suitable accuracy, and precisions of ±0.5 and ±1.1 °C, respectively. By optimizing Er3+ concentration, we could obtain the high green emission intensity, and in turn, high thermal sensitivity for the probe. The probe fabricated in the study exhibited suitable properties for its application as a temperature sensor and superior performance compared to other Er3+ -based optical thermometers in terms of thermal sensitivity.
A Functional IL22 Polymorphism (rs2227473) Is Associated with Predisposition to Childhood Cerebral Malaria
Authors: Marquet Sandrine , Conte Ianina , Poudiougou Belco , Argiro Laurent , Dessein Hélia , Couturier Charlène , Burté Florence , Oumar Aboubacar A. , Brown Biobele J. , Traore Abdoualye , Afolabi Nathaniel K. , Barry Abdoulaye , Omokhodion Samuel , Shokunbi Wuraola A. , Sodeinde Olugbemiro , Doumbo Ogobara , Fernandez-Reyes Delmiro , Dessein Alain J. 
Published: January 2017

AbstractCerebral malaria (CM) is a severe complication ofPlasmodium falciparuminfection. This encephalopathy is characterized by coma and is thought to result from mechanical microvessel obstruction and an excessive activation of immune cells leading to pathological inflammation and blood-brain barrier alterations. IL-22 contributes to both chronic inflammatory and infectious diseases, and may have protective or pathogenic effects, depending on the tissue and disease state. We evaluated whether polymorphisms (n = 46) ofIL22andIL22RA2were associated with CM in children from Nigeria and Mali. Two SNPs ofIL22, rs1012356 (P = 0.016, OR = 2.12) and rs2227476 (P = 0.007, OR = 2.08) were independently associated with CM in a sample of 115 Nigerian children with CM and 160 controls. The association with rs2227476 (P = 0.01) was replicated in 240 nuclear families with one affected child from Mali. SNP rs2227473, in linkage disequilibrium with rs2227476, was also associated with CM in the combined cohort for these two populations, (P = 0.004, OR = 1.55). SNP rs2227473 is located within a putative binding site for the aryl hydrocarbon receptor, a master regulator of IL-22 production. Individuals carrying the aggravating T allele of rs2227473 produced significantly more IL-22 than those without this allele. Overall, these findings suggest that IL-22 is involved in the pathogenesis of CM.
Independent movement of the voltage sensors in KV2.1/KV6.4 heterotetramers
Authors: Bocksteins Elke , Snyders Dirk J. , Holmgren Miguel 
Published: January 2017

AbstractHeterotetramer voltage-gated K+ (KV) channels KV2.1/KV6.4 display a gating charge-voltage (QV) distribution composed by two separate components. We use state dependent chemical accessibility to cysteines substituted in either KV2.1 or KV6.4 to assess the voltage sensor movements of each subunit. By comparing the voltage dependences of chemical modification and gating charge displacement, here we show that each gating charge component corresponds to a specific subunit forming the heterotetramer. The voltage sensors from KV6.4 subunits move at more negative potentials than the voltage sensors belonging to KV2.1 subunits. These results indicate that the voltage sensors from the tetrameric channels move independently. In addition, our data shows that 75% of the total charge is attributed to KV2.1, while 25% to KV6.4. Thus, the most parsimonious model for KV2.1/KV6.4 channels’ stoichiometry is 3:1.
Electrostatic-free piezoresponse force microscopy
Authors: Kim Sungho , Seol Daehee , Lu Xiaoli , Alexe Marin , Kim Yunseok 
Published: January 2017

AbstractContact and non-contact based atomic force microscopy (AFM) approaches have been extensively utilized to explore various nanoscale surface properties. In most AFM-based measurements, a concurrent electrostatic effect between the AFM tip/cantilever and sample surface can occur. This electrostatic effect often hinders accurate measurements. Thus, it is very important to quantify as well as remove the impact of the electrostatic effect on AFM-based measurements. In this study, we examine the impact of the electrostatic effect on the electromechanical (EM) response in piezoresponse force microscopy as a model AFM mode. We quantitatively studied the effects of increasing the external electric field and reducing the spring constant of a cantilever. Further, we explored ways to minimize the electrostatic effect. The results provide broad guidelines for quantitatively analyzing the EM response as well as, eventually, for obtaining the electrostatic-free EM response. The conclusions can be applied to other AFM-based measurements that are subject to a strong electrostatic effect between the AFM tip/cantilever and sample surface, regardless of contact and non-contact modes.
Sounds can boost the awareness of visual events through attention without cross-modal integration
Authors: Pápai Márta Szabina , Soto-Faraco Salvador 
Published: January 2017

AbstractCross-modal interactions can lead to enhancement of visual perception, even for visual events below awareness. However, the underlying mechanism is still unclear. Can purely bottom-up cross-modal integration break through the threshold of awareness? We used a binocular rivalry paradigm to measure perceptual switches after brief flashes or sounds which, sometimes, co-occurred. When flashes at the suppressed eye coincided with sounds, perceptual switches occurred the earliest. Yet, contrary to the hypothesis of cross-modal integration, this facilitation never surpassed the assumption of probability summation of independent sensory signals. A follow-up experiment replicated the same pattern of results using silent gaps embedded in continuous noise, instead of sounds. This manipulation should weaken putative sound-flash integration, although keep them salient as bottom-up attention cues. Additional results showed that spatial congruency between flashes and sounds did not determine the effectiveness of cross-modal facilitation, which was again not better than probability summation. Thus, the present findings fail to fully support the hypothesis of bottom-up cross-modal integration, above and beyond the independent contribution of two transient signals, as an account for cross-modal enhancement of visual events below level of awareness.
Ciphertext-only attack on optical cryptosystem with spatially incoherent illumination: from the view of imaging through scattering medium
Authors: Liao Meihua , He Wenqi , Lu Dajiang , Peng Xiang 
Published: January 2017

AbstractSecurity analysis is important and necessary for a new cryptosystem. In this paper, we evaluate the security risk of the optical cryptosystem with spatially incoherent illumination from the view of imaging through scattering medium and then demonstrate that it is vulnerable to ciphertext-only attack. The proposed ciphertext-only attack method relies on the optical memory effect for speckle correlations, which reveals a fact that the ciphertext’s autocorrelation is essentially identical to the plaintext’s own autocorrelation. Furthermore, by employing of an improved dynamic hybrid input-output phase-retrieval algorithm, we show that a plaintext image can be directly reconstructed from the autocorrelation of its corresponding ciphertext without any prior knowledge about the plaintext or the phase keys. Meanwhile, the theory analysis and experiment results will also be provided to verify the validity and feasibility of our proposed ciphertext-only attack method. To the best of our knowledge, this is the first time to report optical cryptanalysis from the point of view of imaging through scattering medium and we believe this contribution will open up an avenue to deepen the investigation of optical cryptosystems.
Intravenous Formulation of HET0016 Decreased Human Glioblastoma Growth and Implicated Survival Benefit in Rat Xenograft Models
Authors: Jain Meenu , Gamage Nipuni-Dhanesha H. , Alsulami Meshal , Shankar Adarsh , Achyut Bhagelu R. , Angara Kartik , Rashid Mohammad H. , Iskander Asm , Borin Thaiz F. , Wenbo Zhi , Ara Roxan , Ali Meser M. , Lebedyeva Iryna , Chwang Wilson B. , Guo Austin , Bagher-Ebadian Hassan , Arbab Ali S. 
Published: January 2017

AbstractGlioblastoma (GBM) is a hypervascular primary brain tumor with poor prognosis. HET0016 is a selective CYP450 inhibitor, which has been shown to inhibit angiogenesis and tumor growth. Therefore, to explore novel treatments, we have generated an improved intravenous (IV) formulation of HET0016 with HPßCD and tested in animal models of human and syngeneic GBM. Administration of a single IV dose resulted in 7-fold higher levels of HET0016 in plasma and 3.6-fold higher levels in tumor at 60 min than that in IP route. IV treatment with HPßCD-HET0016 decreased tumor growth, and altered vascular kinetics in early and late treatment groups (p < 0.05). Similar growth inhibition was observed in syngeneic GL261 GBM (p < 0.05). Survival studies using patient derived xenografts of GBM811, showed prolonged survival to 26 weeks in animals treated with focal radiation, in combination with HET0016 and TMZ (p < 0.05). We observed reduced expression of markers of cell proliferation (Ki-67), decreased neovascularization (laminin and αSMA), in addition to inflammation and angiogenesis markers in the treatment group (p < 0.05). Our results indicate that HPßCD-HET0016 is effective in inhibiting tumor growth through decreasing proliferation, and neovascularization. Furthermore, HPßCD-HET0016 significantly prolonged survival in PDX GBM811 model.
Controllable Nanotribological Properties of Graphene Nanosheets
Authors: Zeng Xingzhong , Peng Yitian , Lang Haojie , Liu Lei 
Published: January 2017

AbstractGraphene as one type of well-known solid lubricants possesses different nanotribological properties, due to the varied surface and structural characteristics caused by different preparation methods or post-processes. Graphene nanosheets with controllable surface wettability and structural defects were achieved by plasma treatment and thermal reduction. The nanotribological properties of graphene nanosheets were investigated using the calibrated atomic force microscopy. The friction force increases faster and faster with plasma treatment time, which results from the increase of surface wettability and the introduction of structural defects. Short-time plasma treatment increasing friction force is due to the enhancement of surface hydrophilicity. Longer-time plasma treatment increasing friction force can attribute to the combined effects of the enhanced surface hydrophilicity and the generated structural defects. The structural defects as a single factor also increase the friction force when the surface properties are unified by thermal reduction. The surface wettability and the nanotribological properties of plasma-treated graphene nanosheets can recover to its initial level over time. An improved spring model was proposed to elaborate the effects of surface wettability and structural defects on nanotribological properties at the atomic-scale.
Outcomes of Operative Vaginal Delivery during Trial of Labor after Cesarean Delivery
Authors: Brock Clifton , Govindappagari Shravya , Gyamfi-Bannerman Cynthia 
Published: January 2017

Objective The objective of this study is to determine the maternal and neonatal morbidity associated with attempting operative vaginal delivery (OVD) compared with the alternative of a laboring repeat cesarean delivery (LRCD) in women attempting a trial of labor after cesarean delivery (TOLAC).
Methods This is a secondary analysis of a multicenter prospective study designed to assess perinatal outcomes of OVD in women with a prior uterine scar. The study includes women who attempted TOLAC and reached +2 station with a fully dilated cervix. Composites on neonatal and maternal morbidity were compared between women in whom OVD was attempted and those who underwent LRCD by fitting multivariate logistic regression models.
Results In total, 6,489 women attempting TOLAC reached 2+ station with a fully dilated cervix. Of these, 5,640 (86.9%) had a spontaneous vaginal delivery, 762 (11.7%) underwent attempted OVD, and 87 (1.3%) had an LRCD. Compared with attempting OVD, LRCD was associated with greater neonatal morbidity (odds ratio [OR]: 2.41; 95% confidence interval [CI]: 1.13–5.15) and less maternal morbidity (OR: 0.28; 95% CI: 0.14–0.55). Maternal morbidity of OVD is driven by perineal injury.
Conclusion In laboring women with a previous uterine scar, attempting OVD is associated with greater maternal and less neonatal morbidity than LRCD.