bluuebunny/crossref_metadata_2025_split huggingface dataset:
Posterior uveal biopsy and the trans-scleral Essen forceps biopsy technique
Authors: Otti Vanessa , Thaung Caroline , Mudhar Hardeep Singh , Damato Bertil , Sagoo Mandeep S. , Quhill Hibba 
Published: October 2024

Abstract

Background
Differentiating neoplastic and non-neoplastic uveal tumours can present a diagnostic challenge; intra-ocular biopsy may be necessary. The novel trans-scleral Essen Forceps biopsy (TSEB) technique can improve diagnostic yield compared to fine needle aspiration biopsy (FNAB). We present a case demonstrating the technique and its added value. We also review the success rate of TSEB performed at two tertiary eye centres.

Methods
Retrospective case report and consecutive case series from August 2021 to March 2023. Inclusion criteria were patients who underwent TSEB of posterior uveal lesions from Moorfields Eye Hospital and Sheffield Teaching Hospitals in the United Kingdom. The outcomes were biopsy success rate and complication rate

Results
Eleven biopsies met the inclusion criteria. Eight (73%) were successful, which comprised six uveal melanomas, one melanocytoma and one extranodal marginal zone (ENMZ) lymphoma. One TSEB did not yield tissue for histological examination because of perioperative sample handling. Two (18%) biopsies were histologically inconclusive; both were treated as uveal melanoma on clinical grounds or repeat biopsy. The only complication was vitreous loss and retinal hole without retinal detachment in one eye with a very posterior, shallow choroidal lesion.

Conclusion
TSEB is an effective alternative to established biopsy techniques, yielding larger tissue samples than FNAB with intact tissue architecture. We recommend adding TSEB to the armamentarium of the ocular oncologist.
The evolution and maintenance of trioecy with cytoplasmic male sterility
Authors: Nguyen M. T. , Pannell J. R. 
Published: October 2024

AbstractTrioecy, the co-existence of females, males and hermaphrodites, is a rare sexual system in plants that may be an intermediate state in transitions between hermaphroditism and dioecy. Previous models have identified pollen limitation as a necessary condition for the evolution of trioecy from hermaphroditism. In these models, the seed-production and pollen production of females and males relative to those of hermaphrodites, respectively, are compromised by self-fertilization by hermaphrodites under pollen- limitation. Here, we investigate the evolution of trioecy via the invasion of cytoplasmic male sterility (CMS) into androdioecious populations in which hermaphrodites co-occur with males and where the male determiner is linked to a (partial) fertility restorer. We show that the presence of males in a population renders invasion by CMS more difficult. However, the presence of males also facilitates the maintenance of trioecy even in the absence of pollen limitation by negative frequency-dependent selection, because males reduce the transmission of CMS by females by siring sons (which cannot transmit CMS). We discuss our results in light of empirical observations of trioecy in plants and its potential role in the evolution of dioecy.
Accurate formation enthalpies of solids using reaction networks
Authors: Fromsejer Rasmus , Maribo-Mogensen Bjørn , Kontogeorgis Georgios M. , Liang Xiaodong 
Published: October 2024

AbstractCrystalline solids play a fundamental role in a host of materials and technologies, ranging from pharmaceuticals to renewable energy. The thermodynamic properties of these solids are crucial determinants of their stability and therefore their behavior. The advent of large density functional theory databases with properties of solids has stimulated research on predictive methods for their thermodynamic properties, especially for the enthalpy of formation ΔfH. Increasingly sophisticated artificial intelligence and machine learning (ML) models have primarily driven development in this field in recent years. However, these models can suffer from lack of generalizability and poor interpretability. In this work, we explore a different route and develop and evaluate a framework for the application of reaction network (RN) theory to the prediction of ΔfH of crystalline solids. For an experimental dataset of 1550 compounds we are able to obtain a mean absolute error w.r.t ΔfH of 29.6 meV atom−1 using the RN approach. This performance is better than existing ML-based predictive methods and close to the experimental uncertainty. Moreover, we show that the RN framework allows for straightforward estimation of the uncertainty of the predictions.
Quantum state processing through controllable synthetic temporal photonic lattices
Authors: Monika Monika , Nosrati Farzam , George Agnes , Sciara Stefania , Fazili Riza , Marques Muniz André Luiz , Bisianov Arstan , Lo Franco Rosario , Munro William J. , Chemnitz Mario , Peschel Ulf , Morandotti Roberto 
Published: October 2024

AbstractQuantum walks on photonic platforms represent a physics-rich framework for quantum measurements, simulations and universal computing. Dynamic reconfigurability of photonic circuitry is key to controlling the walk and retrieving its full operation potential. Universal quantum processing schemes based on time-bin encoding in gated fibre loops have been proposed but not demonstrated yet, mainly due to gate inefficiencies. Here we present a scalable quantum processor based on the discrete-time quantum walk of time-bin-entangled photon pairs on synthetic temporal photonic lattices implemented on a coupled fibre-loop system. We utilize this scheme to path-optimize quantum state operations, including the generation of two- and four-level time-bin entanglement and the respective two-photon interference. The design of the programmable temporal photonic lattice enabled us to control the dynamic of the walk, leading to an increase in the coincidence counts and quantum interference measurements without recurring to post-selection. Our results show how temporal synthetic dimensions can pave the way towards efficient quantum information processing, including quantum phase estimation, Boson sampling and the realization of topological phases of matter for high-dimensional quantum systems in a cost-effective, scalable and robust fibre-based setup.
Mutual gaze and movement synchrony boost observers’ enjoyment and perception of togetherness when watching dance duets
Authors: Cross Emily S. , Darda Kohinoor M. , Moffat Ryssa , Muñoz Lina , Humphries Stacey , Kirsch Louise P. 
Published: October 2024

AbstractAs social beings, we are adept at coordinating our body movements and gaze with others. Often, when coordinating with another person, we orient ourselves to face them, as mutual gaze provides valuable cues pertaining to attention and intentions. Moreover, movement synchrony and mutual gaze are associated with prosocial outcomes, yet the perceptual consequences of these forms of coordination remain poorly understood. Across two experiments, we assessed how movement synchrony and gaze direction influence observers’ perceptions of dyads. Observers’ behavioural responses indicated that dyads are perceived as more socially connected and are more enjoyable to watch when moving synchronously and facing each other. Neuroimaging results showed modulation of the Action Observation and Theory of Mind networks by movement synchrony and mutual gaze, with more robust brain activity when evaluating togetherness (i.e., active and intentional collaboration) than aesthetic value (i.e., enjoyment). A fuller understanding of the consequences of movement synchrony and mutual gaze from the observer’s viewpoint holds important implications for social perception, in terms of how observers intuit social relationships within dyads, and the aesthetic value derived from watching individuals moving in these ways.
Improving reuse during the development process for web systems
Authors: El-Halawany Ahmed M. , Elminir Hamdy K. , El-Bakry Hazem 
Published: October 2024

AbstractSoftware reuse has emerged as a crucial practice in the software industry, offering significant benefits in time-to-market, and resources management. This is particularly pertinent in web systems development, where the integration of diverse technologies and the varied backgrounds of technical teams pose substantial challenges. The rapid expansion of web systems underscores the urgent need to adopt best practices and methodologies for web system reuse to streamline the development process, reducing effort, cost, and time. This paper aims to identify the key challenges of web system reuse in the context of small and medium-sized software companies in Egypt and Saudi Arabia. Using qualitative research methods, including interviews, focus groups, and participant observations, an empirical study was conducted to examine current reuse practices and understand the root causes of common challenges. Based on the results of the empirical study, a systematic approach was developed to enhance web system reuse during the development process in the context of small and medium-sized software companies in Egypt and Saudi Arabia. The proposed approach addresses critical gaps in current practices, offering practical guidelines to improve efficiency, reduce development time, and enhance overall software quality. This research contributes to the broader discourse on software reuse by providing context-specific insights and adaptable solutions that are relevant to similar markets worldwide.
A non-invasive predictive model based on multimodality ultrasonography images to differentiate malignant from benign focal liver lesions
Authors: Shen Qianqian , Wu Wei , Wang Ruining , Zhang Jiaqi , Liu Liping 
Published: October 2024

AbstractWe have developed a non-invasive predictive nomogram model that combines image features from Sonazoid contrast-enhanced ultrasound (SCEUS) and Sound touch elastography (STE) with clinical features for accurate differentiation of malignant from benign focal liver lesions (FLLs). This study ultimately encompassed 262 patients with FLLs from the First Hospital of Shanxi Medical University, covering the period from March 2020 to April 2023, and divided them into training set (n = 183) and test set (n = 79). Logistic regression analysis was used to identify independent indicators and develop a predictive model based on image features from SCEUS, STE, and clinical features. The area under the receiver operating characteristic (AUC) curve was determined to estimate the diagnostic performance of the nomogram with CEUS LI-RADS, and STE values. The C-index, calibration curve, and decision curve analysis (DCA) were further used for validation. Multivariate and LASSO logistic regression analyses identified that age, ALT, arterial phase hyperenhancement (APHE), enhancement level in the Kupffer phase, and Emean by STE were valuable predictors to distinguish malignant from benign lesions. The nomogram achieved AUCs of 0.988 and 0.978 in the training and test sets, respectively, outperforming the CEUS LI-RADS (0.754 and 0.824) and STE (0.909 and 0.923) alone. The C-index and calibration curve demonstrated that the nomogram offers high diagnostic accuracy with predicted values consistent with actual values. DCA indicated that the nomogram could increase the net benefit for patients. The predictive nomogram innovatively combining SCEUS, STE, and clinical features can effectively improve the diagnostic performance for focal liver lesions, which may help with individualized diagnosis and treatment in clinical practice.
Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with multiple sclerosis
Authors: Ashraf Hajra , Dikarlo Plamena , Masia Aurora , Zarbo Ignazio R. , Solla Paolo , Ijaz Umer Zeeshan , Sechi Leonardo A. 
Published: October 2024

AbstractThe microbial ecology of Mycobacterium avium subspecies paratuberculosis infections (MAP) within the context of Multiple Sclerosis (MS) is largely an unexplored topic in the literature. Thus, we have characterized the compositional and predicted functional differences of the gut microbiome between MS patients with MAP (MAP+) and without (MAP−) infection. This was done in the context of exposome differences (through self-reported filled questionnaires), principally in anthropometric and sociodemographic patterns to gain an understanding of the gut microbiome dynamics. 16S rRNA microbiome profiling of faecal samples (n = 69) was performed for four groups, which differed by disease and MAP infection: healthy cohort (HC) MAP−; HC MAP+ ; MS MAP−; and MS MAP+ . Using a dynamic strategy, with MAP infection and time of sampling as occupancy models, we have recovered the core microbiome for both HC and MS individuals. Additional application of neutral modeling suggests key genera that are under selection pressure by the hosts. These include members of the phyla Actinobacteriota, Bacteroidota, and Firmicutes. As several subjects provided multiple samples, a Quasi Conditional Association Test that incorporates paired-nature of samples found major differences in Archaea. To consolidate treatment groups, confounders, microbiome, and the disease outcome parameters, a mediation analysis is performed for MS cohort. This highlighted certain genera i.e., Sutterella, Akkermansia, Bacteriodes, Gastranaerophilales, Alistipes, Balutia, Faecalibacterium, Lachnospiraceae, Anaerostipes, Ruminococcaceae, Eggerthellaceae and Clostridia-UCG-014 having mediatory effect using disease duration as an outcome and MAP infection as a treatment group. Our analyses indicate that the gut microbiome may be an important target for dietary and lifestyle intervention in MS patients with and without MAP infection.
The skimmed milk proteome of dairy cows is affected by the stage of lactation and by supplementation with polyunsaturated fatty acids
Authors: Veshkini Arash , Hammon Harald M. , Vogel Laura , Viala Didier , Delosière Mylène , Tröscher Arnulf , Déjean Sébastien , Ceciliani Fabrizio , Sauerwein Helga , Bonnet Muriel 
Published: October 2024

AbstractThe impact of nutritional modification to increase functional polyunsaturated fatty acids (PUFA), such as n-3 and n-6 fatty acids (FA) or conjugated linoleic acid (CLA), on milk proteome profile during early lactation remains largely unknown. We used an untargeted proteomics approach to investigate the impact of lactation day and PUFA supplementation on the proteome signature in skimmed milk over the course of early lactation. Sixteen Holstein dairy cows received abomasal infusion of saturated FA (CTRL) or a mixture of essential FA and CLA (EFA + CLA group) from − 63 to + 63 days relative to parturition. Using quantitative proteomics, 479 unique proteins were identified in skimmed milk at days 1, 28, and 63 postpartum. The top discriminating proteins between transition milk (day 1) and mature milk (days 28 and 63), including members of complements (i.e. C2 and C5), growth factor (TGFB2), lipoproteins (i.e. APOE and APOD), and chaperones (i.e. ST13 and CLU), are associated with calves’ immune system and gut development. The EFA + CLA supplementation moderately affected a few proteins associated with regulating mammary glands’ lipogenesis through the (re)assembly of lipoprotein particles, possibly under the PPAR signaling pathway. Collectively, skimmed milk proteome is dynamically regulated initially by cow’s metabolic and physiological changes and to a lesser extent by nutritional PUFA modifications.
A recent surge in global warming is not detectable yet
Authors: Beaulieu Claudie , Gallagher Colin , Killick Rebecca , Lund Robert , Shi Xueheng 
Published: October 2024

AbstractThe global mean surface temperature is widely studied to monitor climate change. A current debate centers around whether there has been a recent (post-1970s) surge/acceleration in the warming rate. Here we investigate whether an acceleration in the warming rate is detectable from a statistical perspective. We use changepoint models, which are statistical techniques specifically designed for identifying structural changes in time series. Four global mean surface temperature records over 1850–2023 are scrutinized within. Our results show limited evidence for a warming surge; in most surface temperature time series, no change in the warming rate beyond the 1970s is detected despite the breaking record temperatures observed in 2023. As such, we estimate the minimum changes in the warming trend required for a surge to be detectable. Across all datasets, an increase of at least 55% is needed for a warming surge to be detectable at the present time.
Complement-mediated killing of Escherichia coli by mechanical destabilization of the cell envelope
Authors: Benn Georgina , Bortolini Christian , Roberts David M , Pyne Alice L B , Holden Séamus , Hoogenboom Bart W 
Published: October 2024

AbstractComplement proteins eliminate Gram-negative bacteria in the blood via the formation of membrane attack complex (MAC) pores in the outer membrane. However, it remains unclear how outer membrane poration leads to inner membrane permeation and cell lysis. Using atomic force microscopy (AFM) on living Escherichia coli (E. coli), we probed MAC-induced changes in the cell envelope and correlated these with subsequent cell death. Initially, bacteria survived despite the formation of hundreds of MACs that were randomly distributed over the cell surface. This was followed by larger-scale disruption of the outer membrane, including propagating defects and fractures, and by an overall swelling and stiffening of the bacterial surface, which precede inner membrane permeation. We conclude that bacterial cell lysis is only an indirect effect of MAC formation; outer membrane poration leads to mechanical destabilization of the cell envelope, reducing its ability to contain the turgor pressure, leading to inner membrane permeation and cell death.
The Nedd4L ubiquitin ligase is activated by FCHO2-generated membrane curvature
Authors: Sakamoto Yasuhisa , Uezu Akiyoshi , Kikuchi Koji , Kang Jangmi , Fujii Eiko , Moroishi Toshiro , Suetsugu Shiro , Nakanishi Hiroyuki 
Published: October 2024

AbstractThe C2-WW-HECT domain ubiquitin ligase Nedd4L regulates membrane sorting during endocytosis through the ubiquitination of cargo molecules such as the epithelial sodium channel (ENaC). Nedd4L is catalytically autoinhibited by an intramolecular interaction between its C2 and HECT domains, but the protein’s activation mechanism is poorly understood. Here, we show that Nedd4L activation is linked to membrane shape by FCHO2, a Bin-Amphiphysin-Rsv (BAR) domain protein that regulates endocytosis. FCHO2 was required for the Nedd4L-mediated ubiquitination and endocytosis of ENaC, with Nedd4L co-localizing with FCHO2 at clathrin-coated pits. In cells, Nedd4L was specifically recruited to, and activated by, the FCHO2 BAR domain. Furthermore, we reconstituted FCHO2-induced recruitment and activation of Nedd4L in vitro. Both the recruitment and activation were mediated by membrane curvature rather than protein–protein interactions. The Nedd4L C2 domain recognized a specific degree of membrane curvature that was generated by the FCHO2 BAR domain, with this curvature directly activating Nedd4L by relieving its autoinhibition. Thus, we show for the first time a specific function (i.e., recruitment and activation of an enzyme regulating cargo sorting) of membrane curvature by a BAR domain protein.
Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes
Authors: Gao Yuan , Feng Jing , Zhang Yansong , Yi Mengyuan , Zhang Lebing , Yan Yan , Zhu Alan Jian , Liu Min 
Published: October 2024

AbstractWingless (Wg)/Wnt signaling plays a critical role in both development and adult tissue homeostasis. In the Drosophila larval wing disc epithelium, the orderly delivery of Wg/Wnt to the apical and basal cell surfaces is essential for wing development. Here, we identified Ehbp1 as the switch that dictates the direction of Wg/Wnt polarized intracellular transport: the Adaptor Protein complex 1 (AP-1) delivers Wg/Wnt to the basolateral cell surface, and its sequestration by Ehbp1 redirects Wg/Wnt for apical delivery. Genetic analyses showed that Ehbp1 specifically regulates the polarized distribution of Wg/Wnt, a process that depends on the dedicated Wg/Wnt cargo receptor Wntless. Mechanistically, Ehbp1 competes with Wntless for AP-1 binding, thereby preventing the unregulated basolateral Wg/Wnt transport. Reducing Ehbp1 expression, or removing the coiled-coil motifs within its bMERB domain, leads to basolateral Wg/Wnt accumulation. Importantly, the regulation of polarized Wnt delivery by EHBP1 is conserved in vertebrates. The generality of this switch mechanism for regulating intracellular transport remains to be determined in future studies.
Vestibular and Balance Considerations in Type 2 Diabetes: A Tutorial on Pertinent Areas and Issues
Authors: Enayati Zakaria , Cacace Anthony T. 
Published: October 2024

Purpose:
The purpose of this study is to describe the effects of diabetes mellitus (DM) on vestibular and balance system functions in humans. Because Type 2 diabetes (T2D) represents the majority of individuals affected by this condition, this subgroup is the main focus of this tutorial.

Method:
Evidence of dysfunction is based on epidemiological, anatomical, physiological, neuroimaging, and clinical findings. Preventative measures, therapeutic interventions, and other mitigating factors are also given consideration.

Results:
Experimental and clinical findings support the notion that T2D damages vestibular and balance systems to the extent that these effects are more prevalent in patients with higher blood glucose levels and longer duration of the disease. Evidence indicates that T2D increases the occurrence and re-occurrence rates of benign paroxysmal positional vertigo, particularly when it occurs in conjunction with hypertension, osteoarthritis, and otologic disorders like Ménière's disease. Type 2 diabetes also impairs vestibular compensation, which is exacerbated by disease duration. Investigational and clinical studies suggest that galvanic stimulation of the vestibular system can be effective in reducing blood glucose levels and improving rehabilitation outcomes.

Conclusion:
Because DM is a chronic metabolic condition affecting cochlear, vestibular, and balance system functions, lowering blood glucose levels through diet, pharmacological interventions, and exercise can be effective in mitigating dysfunction.
Twice‐input variable‐resolution single‐side switching scheme without reset energy for SAR ADC
Authors: Tang Bin , Hu Yunfeng , Huang QingMing , Hu Lexing , Chen Chaoyi , Li Bin , Wu Zhaohui 
Published: October 2024

AbstractA twice‐input variable‐resolution single‐side switching scheme without reset energy is proposed for successive approximation register (SAR) analogue‐to‐digital converters (ADCs). The proposed switching scheme is based on semi‐resting DAC technology to design a four‐array architecture capable of handling twice the swing of the signal input while reducing the capacitor array size. The technique of top‐plate sampling and monotonic shift is utilized so that no switching energy is generated for the first three comparisons. The proposed scheme utilizes full‐capacitor split, bridged switch, and C‐2C dummy capacitor technology, which reduces average switching energy consumption by 99.8% compared to conventional schemes and enables ADC variable resolution function, making the SAR ADC more suitable for IoT applications.