bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Last In–First Out’: seasonal variations of non‐structural carbohydrates, glucose‐6‐phosphate and ATP in tubers of two Arum species
Authors: Petrussa E. , Boscutti F. , Vianello A. , Casolo V. 
Published: November 2017

Abstract

Knowledge on the metabolism of polysaccharide reserves in wild species is still scarce. In natural sites we collected tubers of Arum italicum Mill. and A. maculatum L. – two geophytes with different apparent phenological timing, ecology and chorology – during five stages of the annual cycle in order to understand patterns of reserve accumulation and degradation.

Both the entire tuber and its proximal and distal to shoot portion were utilised. Pools of non‐structural carbohydrates (glucose, sucrose and starch), glucose‐6‐phosphate and ATP were analysed as important markers of carbohydrate metabolism.

In both species, starch and glucose content of the whole tuber significantly increased from sprouting to the maturation/senescence stages, whereas sucrose showed an opposite trend; ATP and glucose‐6‐phosphate were almost stable and dropped only at the end of the annual cycle. Considering the two different portions of the tuber, both ATP and glucose‐6‐phosphate concentrations were higher in proximity to the shoot in all seasonal stages, except the flowering stage.

Our findings suggest that seasonal carbon partitioning in the underground organ is driven by phenology and occurs independently of seasonal climate conditions. Moreover, our results show that starch degradation, sustained by elevated ATP and glucose‐6‐phosphate pools, starts in the peripheral, proximal‐to‐shoot portion of the tuber, consuming starch accumulated in the previous season, as a ‘Last In–First Out’ mechanism of carbohydrate storage.
Salmonella in breeding pigs: Shedding pattern, transmission of infection and the role of environmental contamination in Irish commercial farrow‐to‐finish herds
Authors: Lynch H. , Walia K. , Leonard F. C. , Lawlor P. G. , Manzanilla E. G. , Grant J. , Duffy G. , Gardiner G. E. , Cormican M. , King J. , Markey B. K. , Fanning S. , Argüello H. 
Published: November 2017

SummaryThis study aimed to provide new insights into the epidemiology of Salmonella in pig production, focusing on potential shedding patterns in breeding pigs throughout a full production cycle and the risk of transmission of infection from the sow to her offspring. A longitudinal study was conducted on five farrow‐to‐finish commercial pig farms. In each herd, shedding of Salmonella in faeces was monitored in breeders through service, gestation and lactation. Swabs of the farrowing room floor and pools of faeces from piglets were collected on two occasions during lactation. Environmental pen swabs were also taken in the weaning and finisher houses. Salmonella isolates were serotyped, tested for antimicrobial resistance (AMR) and typed by Multiple‐Locus Variable number tandem repeat Analysis (MLVA). Shedding by breeding pigs was low in all stages of the production cycle; 5% of sows shed at service, the production stage with highest risk of shedding (p < .01), 1.6% shed during gestation and 2.5% after farrowing. Salmonella was detected in 4% of piglet faecal pools in the second week post‐farrowing and 5% in the fourth week. Serotyping and AMR profiles of Salmonella isolates revealed that strains in sows and gilts were mostly different from strains isolated in weaner and finisher facilities. MLVA typing confirmed that the source of infection in piglets was in most instances the contaminated environment rather than their dam. Based on the typing results, it appears that sows do not pose a major risk in the maintenance and transmission of Salmonella to their progeny but instead the contaminated pen environment is more significant in the perpetuation of the organism on farm.
Protein Recommendations for Weight Loss in Elite Athletes: A Focus on Body Composition and Performance
Authors: Hector Amy J. , Phillips Stuart M. 
Published: November 2017

There exists a large body of scientific evidence to support protein intakes in excess of the recommended dietary allowance (RDA) (0.8 g protein/kg/day) to promote the retention of skeletal muscle and loss of adipose tissue during dietary energy restriction. Diet-induced weight loss with as low as possible ratio of skeletal muscle to fat mass loss is a situation we refer to as high-quality weight loss. We propose that high-quality weight loss is often of importance to elite athletes in order to maintain their muscle (engine) and shed unwanted fat mass, potentially improving athletic performance. Current recommendations for protein intakes during weight loss in athletes are set at 1.6–2.4 g protein/kg/day. However, the severity of the caloric deficit and type and intensity of training performed by the athlete will influence at what end of this range athletes choose to be. Other considerations regarding protein intake that may help elite athletes achieve weight loss goals include the quality of protein consumed, and the timing and distribution of protein intake throughout the day. This review highlights the scientific evidence used to support protein recommendations for high-quality weight loss and preservation of performance in athletes. Additionally, the current knowledge surrounding the use of protein supplements, branched chain amino acids (BCAA), β-hydroxy β-methylbutyrate (HMB), and other dietary supplements with weight loss claims will be discussed.
Understanding the resistance to creating safer ice hockey: essential points for injury prevention
Authors: Todd Ryan A , Soklaridis Sophie , Treen Alice K , Bhalerao Shree U , Cusimano Michael D 
Published: November 2017

IntroductionDespite the known negative health outcomes of concussions in minor level boys’ hockey, there has been significant resistance to creating a safer game with less body checking.MethodsTo better understand cultural barriers that prevent making the sport safer for youth and adolescents, semistructured interviews, with 20 ice hockey stakeholders, were conducted and analysed using thematic analysis.ResultsThrough this analysis, two primary concepts arose from respondents. The first concept is that body checking, despite the harm it can cause, should be done in a respectful sportsmanlike fashion. The second concept is the contradiction that the game of ice hockey is both dynamic and unchangeable.DiscussionUsing structural functionalist theory, we propose an argument that the unfortunate perpetuation of violence and body checking in youth ice hockey serves to maintain the social order of the game and its culture. Any strategies aimed at modifying and promoting healthy behaviour in the game should take these concepts into account.
Unintentional injury mortality among indigenous communities of Taiwan: trends from 2002 to 2013 and evaluation of a community-based intervention
Authors: Pan Stephen W , Chong Hiu Ha , Kao Hui-Chuan 
Published: November 2017

IntroductionIndigenous communities in Taiwan shoulder a disproportionate burden of unintentional injury fatalities. We compare unintentional injury mortality rate trends among Taiwan’s indigenous communities and the general population from 2002 to 2013, and evaluate potential impact of a community-based injury prevention programme on indigenous unintentional injury death rates.MethodsStandardised and crude unintentional injury mortality rates were obtained from Taiwan government reports. Segmented linear regression was used to estimate and compare unintentional injury mortality rate trends before and after the intervention.ResultsBetween 2002 and 2013, unintentional injury mortality rates among Taiwan’s indigenous population significantly declined by about 4.5 deaths per 100 000 each year (p<0.0001). During that time, the unintentional injury mortality rate ratio between indigenous Taiwanese and the general population significantly decreased by approximately 1% each successive year (p=0.02). However, we were unable to detect evidence that the ‘Healthy and Safe Tribe’ programme was associated with a statistically significant decrease in the unintentional injury mortality rate trend among indigenous persons (p=0.81).ConclusionTaiwanese indigenous communities remain at significantly higher risk of unintentional injury death, though the gap may be slowly narrowing. We found no evidence that the ‘Healthy and Safe Tribe’ indigenous injury-prevention programme significantly contributed to the nationwide decline in unintentional injury mortality among indigenous Taiwanese communities from 2009 to 2013. Future interventions to address the disproportionate burden of unintentional injury fatalities among indigenous Taiwanese should consider interventions with wider coverage of the indigenous population, and complementing grass roots led community-based interventions with structural policy interventions as well.
Associations between objectively measured physical activity and later mental health outcomes in children: findings from the UK Millennium Cohort Study
Authors: Ahn Jane V , Sera Francesco , Cummins Steven , Flouri Eirini 
Published: November 2017

BackgroundThe beneficial effect of physical activity (PA) on mental health in adults is well established, but less is known about this relationship in children. We examine associations between objectively measured sedentary time, PA and mental health in 11-year-olds from the UK Millennium Cohort Study (MCS).MethodsLongitudinal data from MCS sweeps 4 (age 7) and 5 (age 11) were used (n=6153). Accelerometer data were collected at MCS4, and mental health was measured at MCS4 and MCS5 using subscales (peer, emotional, conduct, hyperactivity) of the Strengths and Difficulties Questionnaire (SDQ). Associations between mean daily PA minutes at different intensities (sedentary, light, moderate-to-vigorous) at MCS4 and SDQ outcomes at MCS5 (score range 0–10) were estimated using multiple linear regression models, adjusting for SDQ at MCS4 and individual and family characteristics, and stratified by gender.ResultsIn fully adjusted models, increased PA at MCS4 was associated with fewer peer problems in boys and girls at MCS5. For each additional 15 min in moderate-to-vigorous physical activity (MVPA), peer problems decreased −0.077 points (95% CI −0.133 to –0.022) in boys. For girls, light PA was associated with decreased peer problems (−0.071 points/30 min, 95% CI −0.130 to –0.013). Greater sedentary time was associated with more peer problems and fewer hyperactivity symptoms in boys and girls. Increased MVPA was associated with more conduct and hyperactivity problems in boys and more hyperactivity in girls.ConclusionsIncreased sedentary time is associated with more peer problems in children, and PA, generally, is beneficial for peer relations in children aged 11.
Association between psychosocial work conditions and latent alcohol consumption trajectories among men and women over a 16-year period in a national Canadian sample
Authors: Dobson Kathleen G , Ibrahim Selahadin , Gilbert-Ouimet Mahée , Mustard Cameron A , Smith Peter M 
Published: November 2017

BackgroundIt is unclear how psychosocial working conditions influence future alcohol consumption. Using group-based trajectory modelling, this study aimed to determine: the number of latent alcohol consumption trajectories over 16 years in a representative sample of the Canadian workforce; the association between psychosocial working conditions and longitudinal alcohol consumption; and if the association between psychosocial work factors and longitudinal alcohol consumption differed among men and women.MethodsWe included 5458 employed adults from the longitudinal Canadian National Population Health Survey. Average daily alcohol consumption was measured every 2 years from 1994 to 2010. Psychosocial work factors were measured in 1994 using the Job Content Questionnaire. Group-based trajectory modelling was used to derive the appropriate number of alcohol behaviour trajectories. The association between psychosocial work factors and alcohol trajectory membership was estimated using multinomial logistic regression. Models were stratified by sex to determine if these associations differed among men and women.ResultsThree alcohol consumption trajectories were present: non-drinkers, light drinkers (0.5–1 drinks/day) and moderate drinkers (2–3 drinks/day). Higher workplace physical exertion and lower social support levels were associated with membership in the moderate drinking trajectory. Among men, lower psychological demands and higher physical exertion levels were associated with membership in the moderate drinking trajectory. Among women, lower levels of physical exertion were associated with membership in the light drinking trajectory, and higher psychological demand levels were associated with membership in the moderate drinking trajectory.ConclusionsOur study suggests that workplace physical exertion and psychological demands may be associated with different alcohol consumption trajectories among men and women.
Total workload and recovery in relation to worktime reduction: a randomised controlled intervention study with time-use data
Authors: Schiller Helena , Lekander Mats , Rajaleid Kristiina , Hellgren Carina , Åkerstedt Torbjörn , Barck-Holst Peter , Kecklund Göran 
Published: November 2017

ObjectivesA 25% reduction of weekly work hours for full-time employees has been shown to improve sleep and alertness and reduce stress during both workdays and days off. The aim of the present study was to investigate how employees use their time during such an intervention: does total workload (paid and non-paid work) decrease, and recovery time increase, when work hours are reduced?MethodsFull-time employees within the public sector (n=636; 75% women) were randomised into intervention group and control group. The intervention group (n=370) reduced worktime to 75% with preserved salary during 18 months. Data were collected at baseline, after 9 months and 18 months. Time-use was reported every half-hour daily between 06:00 and 01:00 during 1 week at each data collection. Data were analysed with multilevel mixed modelling.ResultsCompared with the control group, the intervention group increased the time spent on domestic work and relaxing hobby activities during workdays when worktime was reduced (P≤0.001). On days off, more time was spent in free-time activities (P=0.003). Total workload decreased (−65 min) and time spent in recovery activities increased on workdays (+53 min). The pattern of findings was similar in subgroups defined by gender, family status and job situation.ConclusionsA worktime reduction of 25% for full-time workers resulted in decreased total workload and an increase of time spent in recovery activities, which is in line with the suggestion that worktime reduction may be beneficial for long-term health and stress.
Reassessing the importance of ‘lost pleasure’ associated with smoking cessation: implications for social welfare and policy
Authors: Pechacek Terry Frank , Nayak Pratibha , Slovic Paul , Weaver Scott R , Huang Jidong , Eriksen Michael P 
Published: November 2017

IntroductionBenefit–cost analyses of tobacco regulations include estimates of the informed choice of smokers to continue smoking. Few studies have focused on subjective feelings associated with continued smoking. This study estimates how smoker discontent and regret relate to risk perceptions and health concerns.MethodsWe analysed data from a 2015 nationally representative, online survey of 1284 US adult current smokers. Information was collected on regret, intention to quit, perceived addiction, risk perceptions and health concerns. Multivariate logistic regression adjusting for sociodemographics and health status was used to examine factors associated with smoker discontent.ResultsMore than 80% of current smokers report high (22.5%) or very high (59.8%) discontent due to inability to quit, perceived addiction and regret about having started to smoke. Higher levels of discontent did not vary significantly by sex, age, race/ethnicity, education or income (adjusted odds ratios (AORs) 0.5–1.2). Compared with the smokers expressing low (5.9%) or very low (3.6%) discontent, those expressing higher levels of discontent perceived their health status as fair/poor (AOR=2.3), worried most of the time about lung cancer (AOR=4.6) and felt they were more likely to develop lung cancer in the future (AOR=5.1).ConclusionThe proportion of smokers who might be characterised as having a preference to continue smoking are greatly outnumbered by addicted, discontent and concerned smokers who want to quit and regret ever having started to smoke. These discontent smokers could have a substantial net welfare gain if new regulations helped them escape their concerns about the health effects from continuing smoking.
Time will tell: temporal evolution of Martian gullies and palaeoclimatic implications
Authors: de Haas T. , Conway S. J. , Butcher F. E. G. , Levy J. , Grindrod P. M. , Goudge T. A. , Balme M. R. 
Published: November 2017

Abstract
To understand Martian palaeoclimatic conditions and the role of volatiles therein, the spatiotemporal evolution of gullies must be deciphered. While the spatial distribution of gullies has been extensively studied, their temporal evolution is poorly understood. We show that gully size is similar in very young and old craters. Gullies on the walls of very young impact craters (less than a few myr) typically cut into bedrock and are free of latitude-dependent mantle (LDM) and glacial deposits, while such deposits become increasingly evident in older craters. These observations suggest that gullies go through obliquity-driven degradation–accumulation cycles over time, controlled by: (1) LDM emplacement and degradation; and (2) glacial emplacement and removal. In glacially-influenced craters, the distribution of gullies on crater walls coincides with the extent of glacial deposits, which suggests that the melting of snow and ice played a role in the formation of these gullies. Yet, present-day activity is observed in some gullies on formerly glaciated crater walls. Moreover, in very young craters, extensive gullies have formed in the absence of LDM and glacial deposits, showing that gully formation can also be unrelated to these deposits. The Martian climate varied substantially over time, and the gully-forming mechanisms are likely to have varied accordingly.
The formation of gullies on Mars today
Authors: Dundas Colin M. , McEwen Alfred S. , Diniega Serina , Hansen Candice J. , Byrne Shane , McElwaine Jim N. 
Published: November 2017

Abstract

A decade of high-resolution monitoring has revealed extensive activity in fresh Martian gullies. Flows within the gullies are diverse: they can be relatively light, neutral or dark, colourful or bland, and range from superficial deposits to 10 m-scale topographic changes. We observed erosion and transport of material within gullies, new terraces, freshly eroded channel segments, migrating sinuous curves, channel abandonment, and lobate deposits. We also observed early stages of gully initiation, demonstrating that these processes are not merely modifying pre-existing landforms. The timing of activity closely correlates with the presence of seasonal CO
2
frost, so the current changes must be part of ongoing gully formation that is driven largely by its presence. We suggest that the cumulative effect of many flows erodes alcoves and channels, and builds lobate aprons, with no involvement of liquid water. Instead, flows may be fluidized by sublimation of entrained CO
2
ice or other mechanisms. The frequent activity is likely to have erased any features dating from high-obliquity periods, so fresh gully geomorphology at middle and high latitudes is not evidence for past liquid water. CO
2
ice-driven processes may have been important throughout Martian geological history and their deposits could exist in the rock record, perhaps resembling debris-flow sediments.
TEM8/ANTXR1-Specific CAR T Cells as a Targeted Therapy for Triple-Negative Breast Cancer
Authors: Byrd Tiara T. , Fousek Kristen , Pignata Antonella , Szot Christopher , Samaha Heba , Seaman Steven , Dobrolecki Lacey , Salsman Vita S. , Oo Htoo Zarni , Bielamowicz Kevin , Landi Daniel , Rainusso Nino , Hicks John , Powell Suzanne , Baker Matthew L. , Wels Winfried S. , Koch Joachim , Sorensen Poul H. , Deneen Benjamin , Ellis Matthew J. , Lewis Michael T. , Hegde Meenakshi , Fletcher Bradley S. , St. Croix Brad , Ahmed Nabil 
Published: November 2017

Abstract
Triple-negative breast cancer (TNBC) is an aggressive disease lacking targeted therapy. In this study, we developed a CAR T cell–based immunotherapeutic strategy to target TEM8, a marker initially defined on endothelial cells in colon tumors that was discovered recently to be upregulated in TNBC. CAR T cells were developed that upon specific recognition of TEM8 secreted immunostimulatory cytokines and killed tumor endothelial cells as well as TEM8-positive TNBC cells. Notably, the TEM8 CAR T cells targeted breast cancer stem–like cells, offsetting the formation of mammospheres relative to nontransduced T cells. Adoptive transfer of TEM8 CAR T cells induced regression of established, localized patient-derived xenograft tumors, as well as lung metastatic TNBC cell line–derived xenograft tumors, by both killing TEM8+ TNBC tumor cells and targeting the tumor endothelium to block tumor neovascularization. Our findings offer a preclinical proof of concept for immunotherapeutic targeting of TEM8 as a strategy to treat TNBC.
Significance: These findings offer a preclinical proof of concept for immunotherapeutic targeting of an endothelial antigen that is overexpressed in triple-negative breast cancer and the associated tumor vasculature. Cancer Res; 78(2); 489–500. ©2017 AACR.
Radiotherapy and CTLA-4 Blockade Shape the TCR Repertoire of Tumor-Infiltrating T Cells
Authors: Rudqvist Nils-Petter , Pilones Karsten A. , Lhuillier Claire , Wennerberg Erik , Sidhom John-William , Emerson Ryan O. , Robins Harlan S. , Schneck Jonathan , Formenti Silvia C. , Demaria Sandra 
Published: November 2017

AbstractImmune checkpoint inhibitors activate T cells to reject tumors. Unique tumor mutations are key T-cell targets, but a comprehensive understanding of the nature of a successful antitumor T-cell response is lacking. To investigate the T-cell receptor (TCR) repertoire associated with treatment success versus failure, we used a well-characterized mouse carcinoma that is rejected by CD8 T cells in mice treated with radiotherapy (RT) and anti–CTLA-4 in combination, but not as monotherapy, and comprehensively analyzed tumor-infiltrating lymphocytes (TILs) by high-throughput sequencing of the TCRΒ CDR3 region. The combined treatment increased TIL density and CD8/CD4 ratio. Assessment of the frequency of T-cell clones indicated that anti–CTLA-4 resulted in fewer clones and a more oligoclonal repertoire compared with untreated tumors. In contrast, RT increased the CD8/CD4 ratio and broadened the TCR repertoire, and when used in combination with anti–CTLA-4, these selected T-cell clones proliferated. Hierarchical clustering of CDR3 sequences showed a treatment-specific clustering of TCRs that were shared by different mice. Abundant clonotypes were commonly shared between animals and yet treatment-specific. Analysis of amino-acid sequence similarities revealed a significant increase in the number and richness of dominant CDR3 motifs in tumors treated with RT + anti–CTLA-4 compared with control. The repertoire of TCRs reactive with a single tumor antigen recognized by CD8+ T cells was heterogeneous but highly clonal, irrespective of treatment. Overall, data support a model whereby a diverse TCR repertoire is required to achieve tumor rejection and may underlie the synergy between RT and CTLA-4 blockade. Cancer Immunol Res; 6(2); 139–50. ©2017 AACR.