bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs
Authors: Cuevas Arenas Rodrigo , Danielczak Bartholomäus , Martel Anne , Porcar Lionel , Breyton Cécile , Ebel Christine , Keller Sandro 
Published: April 2017

AbstractSome styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs preserve a lipid-bilayer core, they appear to be more dynamic than other membrane mimics. We used time-resolved Förster resonance energy transfer and small-angle neutron scattering to determine the kinetics and the mechanisms of phospholipid transfer among SMALPs. In contrast with vesicles or protein-bounded nanodiscs, SMALPs exchange lipids not only by monomer diffusion but also by fast collisional transfer. Under typical experimental conditions, lipid exchange occurs within seconds in the case of SMALPs but takes minutes to days in the other bilayer particles. The diffusional and second-order collisional exchange rate constants for SMALPs at 30 °C are kdif = 0.287 s−1 and kcol = 222 M−1s−1, respectively. Together with the fast kinetics, the observed invariability of the rate constants with probe hydrophobicity and the moderate activation enthalpy of ~70 kJ mol−1 imply that lipids exchange through a “hydrocarbon continuum” enabled by the flexible nature of the SMA belt surrounding the lipid-bilayer core. Owing to their fast lipid-exchange kinetics, SMALPs represent highly dynamic equilibrium rather than kinetically trapped membrane mimics, which has important implications for studying protein/lipid interactions in polymer-bounded nanodiscs.
Experimental and theoretical studies of nonlinear dependence of the internal resistance and electrode thickness for high performance supercapacitor
Authors: Liu Xilong , Dai Xiaohang , Wei Guodong , Xi Yunlong , Pang Mingjun , Izotov Volodymyr , Klyui Nickolai , Havrykov Dmytro , Ji Yuan , Guo Qing , Han Wei 
Published: April 2017

AbstractIn this study, the internal resistance with the increasing of electrode thickness in a typical nanoporous carbon-based supercapacitor and their corresponding electrochemical performances were designed and investigated in detail. As for the carbon-based double electrode layer electrochemical system, electrochemical experiments greatly support the fact of nonlinear dependence and indicate that the curve of internal resistance vs. electrode thickness can have a minimum value when the thickness increasing from 10 to 140 μm. To explain the underlying mechanisms responsible for the nonlinear dependence, a theoretical model based on a porous electrode/electrolyte electrochemical system was proposed. As expected, the results of calculations carried out in the framework of the proposed model can be very good agreement with the experimental data. According to the calculation, the optimized electrode thickness is 53.1 μm corresponding to the minimum value of SC internal resistance. Obviously, the current research results might greatly support the nonlinear conclusion instead of linear relationship between the internal resistance and the electrode thickness and may shed some light on the fabrication and exploration of supercapacitors with high power density.
A Microfluidic Immunostaining System Enables Quality Assured and Standardized Immunohistochemical Biomarker Analysis
Authors: Kwon Seyong , Cho Chang Hyun , Kwon Youngmee , Lee Eun Sook , Park Je-Kyun 
Published: April 2017

AbstractImmunohistochemistry (IHC) plays an important role in biomarker-driven cancer therapy. Although there has been a high demand for standardized and quality assured IHC, it has rarely been achieved due to the complexity of IHC testing and the subjective validation-based process flow of IHC quality control. We present here a microfluidic immunostaining system for the standardization of IHC by creating a microfluidic linearly graded antibody (Ab)-staining device and a reference cell microarray. Unlike conventional efforts, our system deals primarily with the screening of biomarker staining conditions for quantitative quality assurance testing in IHC. We characterized the microfluidic matching of Ab staining intensity using three HER2 Abs produced by different manufacturers. The quality of HER2 Ab was also validated using tissues of breast cancer patients, demonstrating that our system is an efficient and powerful tool for the standardization and quality assurance of IHC.
SERS spectral study of HAuCl4-cysteine nanocatalytic reaction and its application for detection of heparin sodium with label-free VB4r molecular probe
Authors: Wang Xiaoliang , Jiang Caina , Qin Yanna , Peng Yutao , Wen Guiqing , Liang Aihui , Jiang Zhiliang 
Published: April 2017

AbstractIn the presence of nanocatalyst, L-cysteine reduce HAuCl4 rapidly to form gold nanoparticles (AuNP), and a quick nanocatalytic preparation procedure was established for Au/AuNP sol with highly active surface enhanced Raman scattering (SERS) effect and good stability. The nanoreaction was also studied by absorption, resonance Rayleigh scattering (RRS), transmission electron microscopy (TEM) and energy spectra. In the selected conditions, the analyte heparin sodium (HS) could react with victoria blue 4 R (VB4r) to form associated complexes which have very weak SERS effect to make the SERS signals decrease. The SERS signals at 1617 cm−1 reduced linearly with HS concentration increasing. Upon addition of FeCl3, it hydrolyzed to form stable Fe(OH)3 sol platform that carried SERS active Au/AuNPs to enhance the sensitivity. Accordingly, we established a SERS quantitative analysis method in the sol substrate of Fe(OH)3-Au/AuNPs, with a linear range of 0.5–75 ng/mL HS and a detection limit of 0.2 ng/mL. HS in real samples was determined, with a relative standard deviation of 2.65–7.63% and a recovery of 99.3–101%.
Inhibition of Pyruvate Kinase M2 Markedly Reduces Chemoresistance of Advanced Bladder Cancer to Cisplatin
Authors: Wang Xing , Zhang Fenglin , Wu Xue-Ru 
Published: April 2017

AbstractChemoresistance to cisplatin is a principal cause of treatment failure and mortality of advanced bladder cancer (BC). The underlying mechanisms remain unclear, which hinders the development of preventive strategies. Recent data indicate that pyruvate kinase M2 (PKM2), a glycolytic enzyme for Warburg effect, is strongly upregulated in BC. This study explores the role of PKM2 in chemoresistance and whether inhibiting PKM2 augments the chemosensitivity to cisplatin and reduces BC growth and progression. We found that Shikonin binds PKM2 and inhibits BC cell survival in a dose-dependent but pyruvate kinase activity-independent manner. Down-regulation of PKM2 by shRNA blunts cellular responses to shikonin but enhances the responses to cisplatin. Shikonin and cisplatin together exhibit significantly greater inhibition of proliferation and apoptosis than when used alone. Induced cisplatin-resistance is strongly associated with PKM2 overexpression, and cisplatin-resistant cells respond sensitively to shikonin. In syngeneic mice, shikonin and cisplatin together, but not as single-agents, markedly reduces BC growth and metastasis. Based on these data, we conclude that PKM2 overexpression is a key mechanism of chemoresistance of advanced BC to cisplatin. Inhibition of PKM2 via RNAi or chemical inhibitors may be a highly effective approach to overcome chemoresistance and improve the outcome of advanced BC.
Activation of perineuronal net-expressing excitatory neurons during associative memory encoding and retrieval
Authors: Morikawa Shota , Ikegaya Yuji , Narita Minoru , Tamura Hideki 
Published: April 2017

AbstractPerineuronal nets (PNNs), proteoglycan-rich extracellular matrix structures, are thought to be expressed around inhibitory neurons and contribute to critical periods of brain function and synaptic plasticity. However, in some specific brain regions such as the amygdala, PNNs were predominantly expressed around excitatory neurons. These neurons were recruited during auditory fear conditioning and memory retrieval. Indeed, the activation of PNN-expressing excitatory neurons predicted cognitive performance.
Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38
Authors: Bajpai Vivek K. , Alam Md Badrul , Quan Khong Trong , Kwon Kyoo-Ri , Ju Mi-Kyoung , Choi Hee-Jeong , Lee Jong Sung , Yoon Jung-In , Majumder Rajib , Rather Irfan A. , Kim Kangmin , Lee Sang-Han , Na MinKyun 
Published: April 2017

AbstractThe aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.
Evaluierung der postoperativen Astigmatismuskorrektur und der postoperativen Rotationsstabilität zweier torischer Intraokularlinsen
Authors: Grohlich M. , Miháltz K. , Lasta M. , Weingessel B. , Vécsei-Marlovits V. 
Published: April 2017

Zusammenfassung
Hintergrund Im Rahmen dieser retrospektiven Studie wurden 2 verschiedene torische Intraokularlinsen auf Rotationsstabilität und das refraktive Ergebnis miteinander verglichen.
Methoden Es handelt sich um eine Analyse von 41 Patientenaugen, die an der Augenabteilung im Krankenhaus Hietzing in den Jahren 2010 bis 2013 operiert wurden. Der korneale Astigmatismus lag über 1,5 Dioptrien (dpt). Bei den implantierten Linsen handelte es sich um die Alcon AcrySof IQ Toric IOL und die Abbott Tecnis Toric Aspheric IOL. Die Ermittlung der Topografie und die Messung der Aberrationen erfolgten mit dem HOYA iTrace™. Die Visusbestimmung erfolgte mit ETDRS-Tafeln. Die gemessenen Abbildungsfehler des untersuchten Auges wurden mit Zernike-Koeffizienten dargestellt.
Ergebnisse Die Rotation in der Alcon-Gruppe war 4,92 ± 4,10° (MW ± SD), in der Abbott-Gruppe 4,31 ± 4,59°. Es zeigte sich hierbei kein signifikanter Unterschied zwischen den beiden torischen Intraokularlinsen, die Rotationsstabilität ist mit anderen Studien vergleichbar. Die refraktive Astigmatismuskorrektur wurde mittels einer Power-Vektoren-Analyse veranschaulicht und zeigte ähnliche Ergebnisse mit eindeutigem Erfolg der Astigmatismuskorrektur. Bei der Alcon IOL zeigte sich ein Restastigmatismus von 0,85 ± 0,48 dpt, bei der Abbott Tecnis IOL war er 1,09 ± 0,66. Der Unterschied ist nicht signifikant. Bei den Abbildungsfehlern (Strehl-Zahl) zeigte sich im Vergleich kein signifikanter Unterschied.
Schlussfolgerung Die Implantation einer torischen Intraokularlinse ist mittlerweile bei regulärem Astigmatismus ein sinnvolles Angebot, das dem Patienten nicht vorenthalten werden sollte. Es zeigte sich hierbei kein signifikanter Unterschied zwischen den beiden torischen Intraokularlinsen.
Paraproteinämische Keratopathie als klinisches Zeichen einer monoklonalen Gammopathie
Authors: Wasielica-Poslednik J. , Gericke A. , Pfeiffer N. , Lisch W. 
Published: April 2017

ZusammenfassungPatienten mit monoklonaler Gammopathie können eine behandlungsbedürftige paraproteinämische Keratopathie (PPK) aufweisen. Die PPK muss gegenüber verschiedenen Formen von Hornhautdystrophie, einzelnen systemischen Stoffwechselstörungen mit Hornhautbeteiligung sowie gegenüber immunologischen und inflammatorischen Hornhauterkrankungen abgegrenzt werden.
A Dynamic Flow Regime Supports an Intact Great Plains Stream Fish Assemblage
Authors: Bestgen Kevin R. , Wilcox Cameron T. , Hill Angela A. , Fausch Kurt D. 
Published: April 2017

AbstractIntact native fish assemblages—those with all or most historical taxa present and a strong numerical dominance of native species—are rare in streams and other aquatic systems worldwide. Our sampling during four intervals from 1983 to 2007 showed that the Purgatoire River, a Great Plains stream in eastern Colorado, supported an assemblage that was 99.6% native taxa, and all but one rare species persisted through time. An additional rare Arkansas River native fish colonized the Purgatoire River and was widespread in 2006–2007, and only three nonnative taxa were detected. The perennial Purgatoire River supported all native fishes in the basin, while tributaries supported mainly native fishes that were tolerant of intermittent, harsh habitat. In the Purgatoire River, frequency and abundance of dominant species varied spatially during 2006–2007. Persistence of native fishes was unchanged over time in the Piñon Canyon reach of the river even though abundance varied substantially. Despite the presence of upstream Trinidad Reservoir, which reduced flood peak magnitude and frequency and increased base flows, the flow regime of the river remained flashy, with high, turbid, and unpredictable flood flows. Peak flows resulted from thunderstorms in tributaries of the Purgatoire River downstream from the reservoir or from snowmelt runoff and usually occurred in August or July but also were observed in May and June. We attribute persistence of the native fish assemblage to sustained base flows and, more importantly, to the continued (albeit lower) magnitude and frequency of peak flows and otherwise harsh habitat, which limit invasion by nonnative fishes from upstream and downstream reservoirs. Persistence of the intact native fish assemblage in the Purgatoire River requires maintenance of the dynamic flow regime and prevention of invasions by nonnative fishes, including riverine‐adapted centrarchids like Smallmouth Bass Micropterus dolomieu. Conservation of the Purgatoire River basin should be a priority for ecologists and managers alike given its value as a study system and as habitat for rare fishes.Received July 18, 2016; accepted March 20, 2017 Published online July 11, 2017
Fish Passage Through Three Types of Structures in Diked Coastal Lake Erie Wetlands
Authors: Steinhart Geoffrey B. , Bode Sierra , Thieme Jennifer , Winslow Christopher J. 
Published: April 2017

Abstract
Over 40% of Great Lakes fishes are wetland dependent, and more than 70% benefit from wetlands periodically. As such, restoration and rehabilitation of Great Lakes coastal wetlands have increased dramatically. This necessitates a balance among varied management objectives, including fish passage. The presence of water control structures commonly found on diked wetlands can limit fish passage if not open all the time. We sampled three diked Lake Erie coastal wetlands to assess how different structures affected fish passage. Water control structures included a pool-and-weir fish ladder, swing gates, and an open culvert. Fyke nets were set five to seven times at each site between April and October 2015. One net was set outside of the wetland, a short distance away from the passage structure, and the other was set inside the wetland, with the net wings enclosing the opening of the structure. Catches were compared for catch rate, three measures of community similarity, and the wetland fish index (WFI). Fish communities on either side of the fish ladder site had low community similarities that were lower than those of the culvert site and similar to those of the swing gate site, suggesting that the fish ladder did not provide fish passage as hoped. Of the three wetlands, catches from inside the wetland with the culvert had a WFI that was most similar to those of wetlands connected to Lake Erie hydrology. Overall, the culvert provided the most opportunity for fish movement into the wetland. However, unlike the other structures, it did not provide a means for precisely managing wetland water levels.
Received September 30, 3016; accepted January 25, 2017 Published online April 4, 2017
Prospects and limitations of antibody‐mediated clearing of lipoproteins from blood plasma prior to nanoparticle tracking analysis of extracellular vesicles
Authors: Mørk Morten , Handberg Aase , Pedersen Shona , Jørgensen Malene M. , Bæk Rikke , Nielsen Morten K. , Kristensen Søren R. 
Published: April 2017

ABSTRACTIntroduction: Nanoparticle tracking analysis (NTA) enables measurement of extracellular vesicles (EVs) but lacks the ability to distinct between EVs and lipoproteins which are abundantly present in blood plasma. Limitations in ultracentrifugation and size exclusion chromatography applied for EV isolation may result in inadequate EV purification and preservation. In this proof of concept study, we aimed to evaluate the potential of antibody‐mediated removal of lipoproteins from plasma prior to extracellular vesicle (EV) analysis by nanoparticle tracking analysis (NTA).Methods: Ten platelet‐free plasma (PFP) samples from healthy fasting subjects were incubated with magnetic beads coated with antibodies against apolipoprotein B‐48 and B‐100 (ApoB). Plasma samples were analysed with NTA before and after application of the bead procedure. Four fasting PFP samples were analysed with an ELISA specific for human ApoB to estimate the degree of removal of lipoproteins and EV array analysis was used for identification of possible EV loss.Results: The magnetic bead separation procedure resulted in a median reduction of the particle concentration in plasma by 62% (interquartile range 32–72%). The mean size of the remaining particles generally increased. ApoB concentration was reduced to a level close to the background signal, whereas a median reduction of the EV content by 21% (range 8–43%) was observed.Conclusion: Anti‐ApoB antibody coated magnetic beads may hold potential for removal of lipoproteins from human PFP prior to EV measurement by NTA but some artefactual effect and EV loss may have to be endured.
Minimization of Food Cost on 2000-Calorie Diabetic Diet
Authors: Urrutia J D , Mercado J , Tampis R L 
Published: April 2017

Abstract
This study focuses on minimization of food cost that satisfies the daily nutrients required based on 2000-calorie diet for a diabetic person. This paper attempts to provide a food combination that satisfies the daily nutrient requirements of a diabetic person and its lowest possible dietary food cost. A linear programming diet model is used to determine the cheapest combination of food items that satisfy the recommended daily nutritional requirements of the diabetic persons. According to the findings, a 50 year old and above diabetic male need to spend a minimum of 72.22 pesos for foods that satisfy the daily nutrients they need. In order to attain the minimum spending, the foods must consist of 60.49 grams of anchovy, 91.24 grams of carrot, 121.92 grams of durian, 121.41 grams of chicken egg, 70.82 grams of pork (lean), and 369.70 grams of rice (well-milled). For a 50 year old and above diabetic female, the minimum spending is 64.65 pesos per day and the food must consist of 75.87 grams of anchovy, 43.38 grams of carrot, 160.46 grams of durian, 69.66 grams of chicken egg, 23.16 grams of pork (lean) and 416.19 grams of rice (well-milled).
Dye adsorption into transition metal-doped zinc oxide nanoparticles supported on natural zeolites to solve wastewater issue
Authors: Pratiwi Margaretha Indra , Afifah Nur , Saleh Rosari 
Published: April 2017

Abstract
In this work, Fe-doped zinc oxide/natural zeolite (Fe:ZnO/NZ) nanocomposites were prepared using the co-precipitation method with various NZ amounts. The nanocomposites were characterized by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) surface area analysis and thermogravimetric analysis (TGA). The nanocomposites were used to remove methylene blue (MB) dye from an aqueous solution. The effect of various NZ amounts and initial MB concentration were tested. The Pseudo-first-order and pseudo-second-order kinetic models were used to explain the adsorption mechanism. The Langmuir and the Freundlich isotherm models were used to fit the adsorption isotherms of the nanocomposites.
Two-dimensional MoS2 under ion irradiation: from controlled defect production to electronic structure engineering
Authors: Ghorbani-Asl Mahdi , Kretschmer Silvan , Spearot Douglas E , Krasheninnikov Arkady V 
Published: April 2017

Abstract
Two-dimensional (2D) transition metal dichalcogenides (TMDs), like MoS2, have unique electronic and optical properties, which can further be tuned using ion bombardment and post-synthesis ion-beam mediated methods combined with exposure of the irradiated sample to precursor gases. The optimization of these techniques requires a complete understanding of the response of 2D TMDs to ion irradiation, which is affected by the reduced dimensionality of the system. By combining analytical potential molecular dynamics with first-principles calculations, we study the production of defects in free-standing MoS2 sheets under noble gas ion irradiation for a wide range of ion energies when nuclear stopping dominates, and assess the probabilities for different defects to appear. We show that depending on the incident angle, ion type and energy, sulfur atoms can be sputtered away predominantly from the top or bottom layers, creating unique opportunities for engineering mixed MoSX compounds where X are chemical elements from group V or VII. We study the electronic structure of such systems, demonstrate that they can be metals, and finally discuss how metal/semiconductor/metal junctions, which exhibit negative differential resistance, can be designed using focused ion beams combined with the exposure of the system to fluorine.