bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaempferol Attenuates Cardiac Hypertrophy via Regulation of ASK1/MAPK Signaling Pathway and Oxidative Stress
Authors: Feng Hong , Cao Jianlei , Zhang Guangyu , Wang Yanggan 
Published: February 2017

AbstractKaempferol has been demonstrated to provide benefits for the treatment of atherosclerosis, coronary heart disease, hyperlipidemia, and diabetes through its antioxidant and anti-inflammatory properties. However, its role in cardiac hypertrophy remains to be elucidated. The aim of our study was to investigate the effects of kaempferol on cardiac hypertrophy and the underlying mechanism. Mice subjected to aorta banding were treated with or without kaempferol (100 mg/kg/d, p. o.) for 6 weeks. Echocardiography was performed to evaluate cardiac function. Mice hearts were collected for pathological observation and molecular mechanism investigation. H9c2 cardiomyocytes were stimulated with or without phenylephrine for in vitro study. Kaempferol significantly attenuated cardiac hypertrophy induced by aorta banding as evidenced by decreased cardiomyocyte areas and interstitial fibrosis, accompanied with improved cardiac functions and decreased apoptosis. The ASK1/MAPK signaling pathways (JNK1/2 and p38) were markedly activated in the aorta banding mouse heart but inhibited by kaempferol treatment. In in vitro experiments, kaempferol also inhibited the activity of ASK1/JNK1/2/p38 signaling pathway and the enlargement of H9c2 cardiomyocytes. Furthermore, our study revealed that kaempferol could protect the mouse heart and H9c2 cells from pathological oxidative stress. Our investigation indicated that treatment with kaempferol protects against cardiac hypertrophy, and its cardioprotection may be partially explained by the inhibition of the ASK1/MAPK signaling pathway and the regulation of oxidative stress.
Reflexivity in the transdisciplinary field of critical discourse studies
Authors: Zienkowski Jan 
Published: February 2017

AbstractThis article outlines an agenda for critical discourse studies that reserves a place of honour for the notion of reflexivity. It draws on four concepts of reflexivity developed in the social sciences: reflexivity as a general feature of interaction and subjectivity; as a methodological praxis; as a property of discursive and non-discursive systems; and as a key feature of late modernity. Reflexivity is considered in terms of acts of interpretive movement. Social actors, organizations and systems throw reflexive loops around themselves, around others, as well as around spatial, temporal, linguistic, cognitive, social and historical dimensions of contextual reality. All social entities engage in the Sisyphean task of fixing social reality, trying to grasp it with a porous and amorphous semiotic net, shaping interpretive reality in the process. Reflexive loops can leave ripples on the surface of language and communication for others to pick up and engage with. Through metadiscursive acts, interlocutors can then engage with the meanings that fix who and what they are in an unequal and power-infused world whose boundaries can only be imagined through interpretive and critical praxis. Reflexivity is a precondition for the articulation of critique and should be considered as a key concept in the field of discourse studies. This paper is published as part of a collection on discourse studies.
Election-related talk and agenda-setting effects on Twitter
Authors: McGregor Shannon C. , Vargo Chris J. 
Published: February 2017

Abstract
This study explores frequency of election-related chatter as an antecedent to agenda setting. In this study, we conducted a longitudinal analysis of 38 million tweets from the 2012 election. Users who participate more in election talk align more with partisan media than less active users. Users who participate less align less with partisan media and more with mainstream media. Overall, agenda-setting relationships differ by participation in election-related talk, with more active users exhibiting a greater agenda-setting effect across all media types. This study provides evidence that as Twitter users talk more about the election, they appear to do so in more homophilous information environments. These environments can alter their perceived importance of issues to match more partisan media. This study echoes previous research that has shown large conversations on Twitter to be more akin to partisan information.
Population Characteristics of Adult Atlantic Sturgeon Captured by the Commercial Fishery in the Saint John River Estuary, New Brunswick
Authors: Dadswell Michael J. , Ceapa Cornel , Spares Aaron D. , Stewart Nathan D. , Curry R. Allen , Bradford Rodney G. , Stokesbury Michael J. W. 
Published: February 2017

AbstractThe commercial fishery for Atlantic Sturgeon Acipenser oxyrinchus in the Saint John River, New Brunswick, began in 1880. The early fishery was unregulated, and the adult stock was depleted by 1886 after landings of 712 metric tons. After a 10‐year closure the fishery was reopened in 1897 with management regulations, and landings varied from 6 to 20 metric tons/year until 2010. In 2011 an annual quota of 350 adults was established and landings are now stable at 11.3 ± 1.7 (mean ± SD) metric tons/year. Since 2009, fishers have collected biological statistics from adults taken in the fishery and 14–60% of captured individuals have been marked and released each year. During 2009–2015, annual mean values of total length and dressed weight of landed adults were stable, the male : female sex ratio was 1.2:1.0, and mean age of males and females was 27.2 and 34.0 years, respectively. Estimates of instantaneous total mortality ranged from 0.08 to 0.11, and mean annual survival was 90.9%. Of 1,396 marked adults released, 147 were recaptured in the estuary in subsequent years. Tag returns indicated that the modal spawning periodicity of males was 2 years and that of females was 4 years. Valid, modified Schnabel and Jolly–Seber mean annual population estimates for 2013–2015 were 18,179 and 20,798 adults, respectively. The quota in relation to these estimated adult populations represented annual exploitation rates of 1.9% and 1.7%, which are below F50 and would maintain present stock size. The virgin adult population was determined using 1880–1886 total landings and a mean weight range for adults of 50–30 kg. Estimated range of the 1880 virgin population size was 14,240–23,733 adults. These data suggest that the fishery is sustainable at its current annual yield and that the population is near the carrying capacity of the Saint John River.Received July 12, 2016; accepted November 17, 2016 Published online February 21, 2017
Effects of alginate oligomer on the expression of cell cycle- and stress-related genes in Chlamydomonas reinhardtii
Authors: Ueno Mikinori , Nishiguchi Tomoki , Takeshita Satoshi , Yamaguchi Kenichi , Oda Tatsuya 
Published: February 2017

Abstract
Enzymatically prepared alginate oligomer (AO) promoted the growth of Chlamydomonas reinhardtii in a concentration-dependent manner. AO at 2.5 mg/mL induced increase in expression levels of cyclin A, cyclin B, and cyclin D in C. reinhardtii. CuSO4 at 100 μM suppressed the growth of C. reinhardtiin, and AO at 2.5 mg/mL significantly alleviated the toxicity of CuSO4. Increased intracellular reactive oxygen species level in C. reinhardtii induced by CuSO4 was reduced by AO. After cultivation with CuSO4 at 100 μM, expression levels of ascorbate peroxidase and superoxide dismutase in C. reinhardtii were increased, and AO reduced the increased levels of these enzymes. These results suggest that AO exhibits beneficial effects on C. reinhardtii through influencing the expression of various genes not only at normal growth condition but also under CuSO4 stress.
Salinity Has Limited Influence on Sedation of Sunshine Bass with AQUI-S 20E (10% Eugenol)
Authors: Barry Kelli J , Trushenski Jesse T , Bowker James D 
Published: February 2017

Abstract
We evaluated sedation and recovery of juvenile sunshine bass (male Striped Bass Morone saxatilis × female White Bass M. chrysops) exposed to AQUI-S 20E (10% eugenol; 60 mg/L) in freshwater or increasingly brackish salinities (nominal salinities of 0, 5, 10, 15, 20, and 25 g/L). Induction and recovery times were significantly affected by salinity, but the magnitude of the effects was relatively small. Induction was fastest (0.90 min) among fish tested at 25 g/L salinity and slowest (1.07 min) among those tested at 0 g/L salinity, but this was the only significant difference observed in induction times between treatments. Recovery was also faster among fish that were tested at higher salinities and slower among fish that were tested at lower salinities (range = 2.63–3.91 min), but significant differences were not noted for each pairwise comparison. Most fish (65%) exhibited slight agitation and gasping or piping at the surface during sedation, but these behaviors were less common among fish held at the highest salinities. Almost all fish (93%) behaved normally during recovery. Minor differences in timing aside, our results demonstrate that salinities ranging from 0 to 25 g/L have little practical influence on the effectiveness of AQUI-S 20E as a sedative for sunshine bass.
Risk Factors for Ineffectiveness of Posterior Decompression and Dekyphotic Corrective Fusion with Instrumentation for Beak-Type Thoracic Ossification of the Posterior Longitudinal Ligament: A Single Institute Study
Authors: Imagama Shiro , Ando Kei , Ito Zenya , Kobayashi Kazuyoshi , Hida Tetsuro , Ito Kenyu , Tsushima Mikito , Ishikawa Yoshimoto , Matsumoto Akiyuki , Morozumi Masayoshi , Tanaka Satoshi , Machino Masaaki , Ota Kyotaro , Nakashima Hiroaki , Wakao Norimitsu , Nishida Yoshihiro , Matsuyama Yukihiro , Ishiguro Naoki 
Published: February 2017

Abstract

BACKGROUND: Thoracic ossification of the posterior longitudinal ligament (T-OPLL) is treated surgically with instrumented posterior decompression and fusion. However, the factors determining the outcome of this approach and the efficacy of additional resection of T-OPLL are unknown.

OBJECTIVE: To identify these factors in a prospective study at a single institution.

METHODS: The subjects were 70 consecutive patients with beak-type T-OPLL who underwent posterior decompression and dekyphotic fusion and had an average of 4.8 years of follow-up (minimum of 2 years). Of these patients, 4 (6%; group R) had no improvement or aggravation, were not ambulatory for 3 weeks postoperatively, and required additional T-OPLL resection; while 66 (group N) required no further T-OPLL resection. Clinical records, gait status, intraoperative ultrasonography, intraoperative neurophysiological monitoring (IONM), plain radiography, computed tomography and magnetic resonance imaging findings, and Japanese Orthopaedic Association (JOA) score were compared between the groups.

RESULTS: Preoperatively, patients in group R had significantly higher rates of severe motor paralysis, nonambulatory status, positive prone and supine position test, no spinal cord floating in intraoperative ultrasonography, and deterioration of IONM at the end of surgery (P < .05). In preoperative radiography, the OPLL spinal cord kyphotic angle difference in fused area, OPLL length, and OPLL canal stenosis were significantly higher in group R (P < .05). At final follow-up, JOA scores improved similarly in both groups.

CONCLUSION: Preoperative severe motor paralysis, nonambulatory status, positive prone and supine position test, radiographic spinal cord compression due to beak-type T-OPLL, and intraoperative residual spinal cord compression and deterioration of IONM were associated with ineffectiveness of posterior decompression and fusion with instrumentation. Our 2-stage strategy may be appropriate for beak-type T-OPLL surgery.
Stroke-associated pattern of gene expression previously identified by machine-learning is diagnostically robust in an independent patient population
Authors: O’Connell Grant C. , Chantler Paul D. , Barr Taura L. 
Published: February 2017

AbstractPurposeOur group recently identified a ten gene pattern of differential expression in peripheral blood which may have utility for detection of stroke. The objective of this study was to assess the diagnostic capacity and temporal stability of this stroke-associated transcriptional signature in an independent patient population.MethodsPublically available whole blood microarray data generated from 23 ischemic stroke patients at 3, 5, and 24 hours post-symptom onset, along with 23 cardiovascular disease controls were obtained via the National Center for Biotechnology Information Gene Expression Omnibus. Expression levels of the ten candidate genes were extracted, compared between groups, and evaluated for their discriminatory ability at each time point.ResultsWe observed a largely identical pattern of differential expression between stroke patients and controls across the ten candidate genes as reported in our prior work. Furthermore, the coordinate expression levels of the ten candidate genes were able to discriminate between stroke patients and controls with levels of sensitivity and specificity upwards of 90% across all three time points.ConclusionsThese findings confirm the diagnostic robustness of the previously identified pattern of differential expression in independent patient population, and further suggest that it is temporally stable over the first 24 hours of stroke pathology.
Niche construction in evolutionary theory: the construction of an academic niche?
Authors: Gupta Manan , Prasad N. G. , Dey Sutirth , Joshi Amitabh , Vidya T. N. C. 
Published: February 2017

AbstractIn recent years, fairly far-reaching claims have been repeatedly made about how niche construction, the modification by organisms of their environment, and that of other organisms, represents a vastly neglected phenomenon in ecological and evolutionary thought. The proponents of this view claim that the niche construction perspective greatly expands the scope of standard evolutionary theory and that niche construction deserves to be treated as a significant evolutionary process in its own right, almost at par with natural selection. Claims have also been advanced about how niche construction theory represents a substantial extension to, and re-orientation of, standard evolutionary theory, which is criticized as being narrowly gene-centric and ignoring the rich complexity and reciprocity of organism-environment interactions. We examine these claims in some detail and show that they do not stand up to scrutiny. We suggest that the manner in which niche construction theory is sought to be pushed in the literature is better viewed as an exercise in academic niche construction whereby, through incessant repetition of largely untenable claims, and the deployment of rhetorically appealing but logically dubious analogies, a receptive climate for a certain sub-discipline is sought to be manufactured within the scientific community. We see this as an unfortunate, but perhaps inevitable, nascent post-truth tendency within science.
NIMA-related kinase 1 (NEK1) regulates the localization and phosphorylation of α-Adducin (ADD1) and Myosin X (MYO10) during meiosis
Authors: Brieño-Enríquez Miguel A. , Moak Stefannie L. , Holloway J. Kim , Cohen Paula E. 
Published: February 2017

Summary statement:NEK1 kinase regulates the assembly and function of the meiosis I spindle by phosphorylating α-adducin (ADD1) and thereby facilitating its interaction with Myosin X (MYO10)Abstract:NIMA-related kinase 1 (NEK1) is a serine/threonine and tyrosine kinase that is highly expressed in mammalian germ cells. Mutations inNek1induce anemia, polycystic kidney and infertility. In this study we evaluated the role of NEK1 in meiotic spindle formation in both male and female gametes. Our results show that the lack of NEK1 provokes an abnormal organization of the meiosis I spindle characterized by elongated and/or multipolar spindles, and abnormal chromosome congression. The aberrant spindle structure is concomitant with the disruption in localization and protein levels of myosin X (MYO10) and α-adducin (ADD1), both of which are implicated in the regulation of spindle formation during mitosis. Interaction of ADD1 with MYO10 is dependent on phosphorylation, whereby phosphorylation of ADD1 enables its binding to MYO10 on mitotic spindles. Reduction in ADD1 protein in NEK1 mutant mice is associated with hyperphosphorylation of ADD1, thereby preventing the interaction with MYO10 during meiotic spindle formation. Our results reveal a novel regulatory role for NEK1 in the regulation of spindle architecture and function during meiosis.
Cas9-Assisted Targeting of CHromosome segments (CATCH) for targeted nanopore sequencing and optical genome mapping
Authors: Gabrieli Tslil , Sharim Hila , Michaeli Yael , Ebenstein Yuval 
Published: February 2017

ABSTRACTVariations in the genetic code, from single point mutations to large structural or copy number alterations, influence susceptibility, onset, and progression of genetic diseases and tumor transformation. Next-generation sequencing analysis is unable to reliably capture aberrations larger than the typical sequencing read length of several hundred bases. Long-read, single-molecule sequencing methods such as SMRT and nanopore sequencing can address larger variations, but require costly whole genome analysis. Here we describe a method for isolation and enrichment of a large genomic region of interest for targeted analysis based on Cas9 excision of two sites flanking the target region and isolation of the excised DNA segment by pulsed field gel electrophoresis. The isolated target remains intact and is ideally suited for optical genome mapping and long-read sequencing at high coverage. In addition, analysis is performed directly on native genomic DNA that retains genetic and epigenetic composition without amplification bias. This method enables detection of mutations and structural variants as well as detailed analysis by generation of hybrid scaffolds composed of optical maps and sequencing data at a fraction of the cost of whole genome sequencing.
Family business and value-added distribution: a socioemotional wealth approach
Authors: Gallizo Jose Luis , Mar-Molinero Cecilio , Moreno Jordi , Salvador Manuel 
Published: February 2017

PurposeResearch has demonstrated that family businesses limit the goal of maximizing profits in exchange for maintaining control of the company and passing control to future generations. However, these decisions are not always shared by the stakeholders who are outside the family context, making tensions arise within the company that may affect profitability and the share prices of the family business. The purpose of this paper is to analyse the internal tensions in family businesses in the value-added (VA) distribution, and whether these tensions harm their performance as a result of the restrictions under which these companies operate.Design/methodology/approachA factor analysis has been used to measure the tension that results from VA distribution of a sample of 105 Spanish listed firms for the 2005-2012 period. A regression analysis has been used to study the impact of this tension on their share prices.FindingsResults show that being a family business has a positive effect on the business tension factor and that returns and share prices are inversely related to tension factors. Thus, the authors conclude that the decision to maintain control over the family business threatens profitability and share prices.Social implicationsAn analysis of distribution of VA in family businesses sheds light on whether or not the management in its decisions preserves its socioemotional wealth (SEW) generating tensions among its economic agents, affecting its profitability and continuity. This knowledge is important for company stakeholders and future investors.Originality/valueThis is the first study in which the value-added statement is used to analyse how the management style of firms, and especially family businesses, are seeking to preserve their SEW and internal tensions generated by them.
Internal marketing and customer-contact employees’ attitudinal outcomes
Authors: Hernández-Díaz Arleen , Calderon-Abreu Theany , Amador-Dumois Maria , Córdova-Claudio Mario 
Published: February 2017

PurposeHigher education institutions, particularly in the case of public universities, face the challenge of creating more value in an environment of increasing requirements and limitations. The purpose of this paper is to explore the relation of internal marketing (IM) with employees’ attitudinal outcomes, which aim to stimulate and retain motivated and customer-conscious contact employees.Design/methodology/approachSurvey data of 94 customer-contact employees in a public higher education institution were analysed using partial least squares path modelling to explore and predict key target constructs.FindingsThe results demonstrate the relationship between customer-contact employees’ service empathy (SE), institutional IM initiatives and employees’ attitudinal outcomes in the public sector, specifically higher education institutions.Research limitations/implicationsThe current study relies on self-reported data and a small sample of customer-contact employees working only on enrolment-related areas. Further studies should be designed for theory confirmation and generalizability of the results.Practical implicationsTop managers in public higher education institutions must encourage organizational identification through IM initiatives, such as well-established internal communication procedures. Academic management should continuously train and retain customer-contact employees that have developed SE and a sense of belonging to the organization. SE must be incorporated in the job descriptions and training of customer-contact employees.Originality/valueThe study contributes to the limited literature on the use of IM and SE in the public sector, specifically higher education institutions.
The mediating role of supply chain collaboration on the relationship between technology, trust and operational performance
Authors: Salam Mohammad Asif 
Published: February 2017

Purpose
The purpose of this paper is to understand the link between trust, technology and supply chain collaboration and their impact on firms’ operational performances.

Design/methodology/approach
Based on extant literature, a hypothesized model was developed and tested using structural equation modelling (i.e. AMOS). A survey was conducted to collect data from the supply chain managers of fast-moving consumer goods (FMCG) companies (more precisely in the food and beverage sector) in Thailand.

Findings
The study findings suggest that through an ongoing relationship, trust evolves and is shaped over time and can form a competitive capability that may not be easy for competitors to replicate. Both trust and technology are found to have significant impact on supply chain collaboration and on firms’ operational performances.

Research limitations/implications
One of the major limitations of the study is that the data were obtained from one single economy, which restricts its generalizability across other economies. The study was a cross-sectional and descriptive sample of the FMCG industry at a given point in time. A more stringent test of the relationships between trust, technology, supply chain collaboration and operational performance requires an in-depth case study or longitudinal study.

Practical implications
The findings will enable supply chain managers in the FMCG industry to understand the strategic role of trust and technology in building supply chain collaboration that leads to superior operational performance.

Originality/value
The paper clearly confirms that trust and technological capabilities are significantly associated with supply chain collaboration and operational performance. Most of the earlier supply chain collaboration research streams are based on developed economies where supply chain collaboration challenges are predictable, while the current study conducted is based on a developing economy setting. This study facilitates the author’s understanding of the challenges in managing supply chain collaboration within a developing economy.