bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mass spectrometry analysis of adipose-derived stem cells reveals a significant effect of hypoxia on pathways regulating extracellular matrix
Authors: Riis Simone , Stensballe Allan , Emmersen Jeppe , Pennisi Cristian Pablo , Birkelund Svend , Zachar Vladimir , Fink Trine 
Published: April 2016

Abstract
Background
Adipose-derived stem cells (ASCs) are being increasingly recognized for their potential to promote tissue regeneration and wound healing. These effects appear to be partly mediated by paracrine signaling pathways, and are enhanced during hypoxia. Mass spectrometry (MS) is a valuable tool for proteomic profiling of cultured ASCs, which may help to reveal the identity of the factors secreted by the cells under different conditions. However, serum starvation which is essentially required to obtain samples compatible with secretome analysis by MS can have a significant influence on ASCs. Here, we present a novel and optimized culturing approach based on the use of a clinically relevant serum-free formulation, which was used to assess the effects of hypoxia on the ASC proteomic profile.

Methods
Human ASCs from three human donors were expanded in StemPro® MSC SFM XenoFree medium. Cells were cultured for 24 h in serum- and albumin-free supplements in either normoxic (20 %) or hypoxic (1 %) atmospheres, after which the cells and conditioned medium were collected, subfractionated, and analyzed using MS. Prior to analysis, the secreted proteins were further subdivided into a secretome (>30 kDa) and a peptidome (3–30 kDa) fraction.

Results
MS analysis revealed the presence of 342, 98, and 3228 proteins in the normoxic ASC secretome, peptidome, and proteome, respectively. A relatively small fraction of the proteome (9.6 %) was significantly affected by hypoxia, and the most regulated proteins were those involved in extracellular matrix (ECM) synthesis and cell metabolism. No proteins were found to be significantly modulated by hypoxic treatment across all cultures for the secretome and peptidome samples.

Conclusions
This study highlights ECM remodeling as a significant mechanism contributing to the ASC regenerative effect after hypoxic preconditioning, and further underscores considerable inter-individual differences in ASC response to hypoxia. The novel culture paradigm provides a basis for future proteomic studies under conditions that do not induce a stress response, so that the best responders can be accurately identified for prospective therapeutic use.
Data are available via ProteomeXchange with identifier PXD003550.
European social citizenship and the privatisation of welfare state
Authors: Katrugalos Georges 
Published: April 2016

<p>The paper tries to outline the recent trends of privatization of public pension schemes in Europe and then to proceed to a critical appraisal of them. Market principles, such as competitiveness and the maximisation of cost efficiency, are becoming more prominent across Europe, contradicting those principles that have traditionally associated with social policy, like equity, solidarity, social justice. However, private and public sectors are not functionally equivalent. There are some roles, as, for instance, redistribution, that only the State can fulfil. Besides that, public values, such as citizenship, in the Marshallean sense, are not easily transferable in privatized environment</p>
Η ευρωπαϊκή κοινωνική έρευνα: Οργανωτική δομή και μεθοδολογικό πλαίσιο
Authors: Υφαντόπουλος Γιάννης Νικ. 
Published: April 2016

<p>Over the last three to four decades, the European Commission and Eurostat have developed harmonized methodologies aiming at the collection and statistical analysis of European data. This process has contributed enormously to<br />the shaping and evaluation of social and economic policies as well as to the assessment of the Lisbon Strategy. The European Social Survey (ESS) constitutes a “New Scientific Instrument” for the investigation of beliefs, attitudes, ideas and social values of the Europeans. Currently, the total number of participating countries amounts to 32 and the data set includes more than 30,000 face to face interviews. The ESS data base is explored by more than 10,000 registered users, producing books, scientific articles, papers and policy documents. In Greece the first round (2002-2003) was conducted by face to face interviews in a random sample of 3.227 households and a response rate of 80%. The second round (2004-2003) was launched to 3.002 households and the response rate accounted to 74,2%. Greece, in comparison with the rest of the participating countries in the ESS study, fulfilled all the strict scientific criteria and achieved the highest response rates.</p>
Εκπαίδευση και συνδικαλιστικές οργανώσεις: όψεις της ευρωπαϊκής εμπειρίας και σύγχρονη ελληνική πραγματικότητα
Authors: Φωτόπουλος Nίκος 
Published: April 2016

<p>Τrade union education is a developed scientific field in most European countries. The need for a systematic performance of trade union duties was one of the main reasons for the creation of specific trade union education providers in order to strengthen the role of trade unions in social dialogue. This article aims to make reference to the European experience, taking into account the establishment of the Greek Labour Academy, in order to further the debate on the role of trade union education in an era of change for the trade union movement in Greece.</p>
Review: Climate-induced hydrological changes and the ecology of tropical freshwater biota
Authors: SUNARDI SUNARDI , WIEGLEB GERHARD 
Published: April 2016

Sunardi, Wiegleb G. 2016. Climate-induced hydrological changes and the ecology of tropical freshwater biota. BiodiversitasÂ 17: 322-331. Climate change is believed to pose adverse effects to biodiversity of aquatic systems, with no exception of those in tropicalÂ areas. However, next few decades species extinction is suggested as dark future as we lack researches uncovering how climate changeÂ threats the aquatic biota. Unluckily, the tropical freshwater systems are expected to suffer more severe impacts of climate change, fromÂ heavy floods or extended drought than do the boreal areas. A comprehensive understanding of biotaâ€™ performance in face of climaticÂ pressures, will guide the further necessary researches. This paper presents a review on the available researches addressing ecologicalÂ effects of the most influential climatic parameter in tropic area, the hydrological regime, on freshwater biota. The research reveal thatÂ the extreme water fluctuations induced by climate change have negatively affected the performance of freshwater biota. In the next fewÂ decades, climate change seems to remain as one of the main threats for freshwater ecosystems, and is responsible for the lost of itsbiodiversity.
Immune responses in rats and sheep induced by a DNA vaccine containing the phosphoglycerate kinase gene of Fasciola hepatica and liver fluke infection
Authors: Wesołowska Agnieszka , Zawistowska-Deniziak Anna , Norbury Luke J. , Wilkowski Przemysław , Januszkiewicz Kamil , Pyziel Anna M. , Zygner Wojciech , Wędrychowicz Halina 
Published: April 2016

AbstractImmune responses of rats and sheep following vaccination with cDNA encoding phosphoglycerate kinase of
Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil
Authors: Fabres Laura Fuhrich , Rosa dos Santos Sayonara Peixoto , Benitez Lisianne Brittes , Rott Marilise Brittes 
Published: April 2016

AbstractFree-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease:
A new species of Spauligodon (Nematoda; Oxyuroidea; Pharyngodonide) and other Helminths in Ptychozoon Kuhli (Squamata: Gekkonidae) from East Malaysia
Authors: Bursey Charles R. , Goldberg Stephen R. , Lee Grismer L. 
Published: April 2016

Abstractsp. nov. from the intestines of
Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia
Authors: Ilić Tamara , Becskei Zsolt , Petrović Tamaš , Polaček Vladimir , Ristić Bojan , Milić Siniša , Stepanović Predrag , Radisavljević Katarina , Dimitrijević Sanda 
Published: April 2016

AbstractWild canides have a high epizootiological – epidemiological significance, considering that they are hosts for some parasites which spread vector born diseases. Increased frequency of certain interactions between domestic and wild canides increases the risk of occurrence, spreading and maintaining the infection of parasitic etiology in domestic canides. The research was conducted in 232 wild canides (172 red foxes and 60 golden jackals). The examined material was sampled from foxes and jackals, which were hunted down between 2010 and 2014, from 8 epizootiological areas of Serbia (North-Bačka, West-Bačka, Southern-Banat, Moravički, Zlatiborski, Raški, Rasinski and Zaječarski district). On completing the parasitological dissection and the coprological diagnostics, in wild canides protozoa from the genus
Seroprevalence of Toxoplasma gondii among turkeys on family farms in the state of Northeastern Brazil
Authors: Sá Silvio G. , Lima Débora C.V. , Silva Luana T.R. , Pinheiro Júnior José W. , Dubey Jitender P. , Silva Jean C.R. , Mota Rinaldo A. 
Published: April 2016

AbstractThe aim of the present study was to investigate the prevalence of antibodies against
Determination of PCT on admission is a useful tool for the assessment of disease severity in travelers with imported Plasmodium falciparum malaria
Authors: Righi Elda , Merelli Maria , Arzese Alessandra , Siega Paola Della , Scarparo Claudio , Bassetti Matteo 
Published: April 2016

AbstractProcalcitonin (PCT) and C-reactive protein (CRP) may be useful to predict complicated forms of malaria. A total of 30 consecutive travelers diagnosed with
Mass spectrometry analysis of the excretory-secretory (E-S) products of the model cestode Hymenolepis diminut a reveals their immunogenic properties and the presence of new E-S proteins in cestodes
Authors: Bień Justyna , Sałamatin Rusłan , Sulima Anna , Savijoki Kirsi , Bruce Conn David , Näreaho Anu , Młocicki Daniel 
Published: April 2016

Abstractis an important model species in studies of therapeutics, biochemical processes, immune responses and other aspects of cestodiasis. The parasite produces numerous excretory-secretory (E-S) proteins and a glycocalyx covering its body. Our study focused on the mass spectrometry analysis of the E-S material with an objective to determine if E-S contains any new proteins, in particular those that can be identified as: antigens, vaccine candidates and drug targets. These proteins might engage directly in host-parasite interactions. Adult parasites collected from experimentally infected rats were cultured
The intact Kunitz domain protects the amyloid precursor protein from being processed by matriptase-2
Authors: Beckmann Anna-Madeleine , Glebov Konstantin , Walter Jochen , Merkel Olaf , Mangold Martin , Schmidt Frederike , Becker-Pauly Christoph , Gütschow Michael , Stirnberg Marit 
Published: April 2016

AbstractProteolytic processing of the amyloid precursor protein (APP) leads to amyloid-β (Aβ) peptides. So far, the mechanism of APP processing is insufficiently characterized at the molecular level. Whereas the knowledge of Aβ generation by several proteases has been expanded, the contribution of the Kunitz-type protease inhibitor domain (KPI) present in two major APP isoforms to the complex proteolytic processing of APP is poorly understood. In this study, we have identified KPI-containing APP as a very potent, slow-binding inhibitor for the membrane-bound proteolytic regulator of iron homeostasis matriptase-2 by forming stable complexes with its target protease in HEK cells. Inhibition and complex formation depend on the intact KPI domain. By inhibiting matriptase-2, KPI-containing APP is protected from matriptase-2-mediated proteolysis within the Aβ region, thus preventing the generation of N-terminally truncated Aβ.
Does step length adjustment determine take-off accuracy and approach run velocity in long and triple jumps?
Authors: Makaruk Hubert , Starzak Marcin , Sadowski Jerzy 
Published: April 2016

PurposeWhile take-off accuracy and approach run velocity are known determinants of long and triple jump performance, the interaction of these factors with step length adjustment (SLA) is not as clear.MethodsThe study involved 39 male and 31 female national-level long and triple jumpers. The Optojump Next device was used to analyse jump attempts. Three groups were identified according to maximum variability of footfall placement (HVF - high, MVF - medium, LVF - Low) as well as three groups regarding the onset of step length adjustment (ESLA - early, MSLA - mid, LSLA - late).ResultsTake-off accuracy in the LVF and MVF groups was greater compared with the HVF group among females. Among males, the LVF group made significantly (<i>p</i> < 0.05) fewer foul attempts than the HVF group. The ESLA group achieved significantly (<i>p</i> < 0.05) higher velocity during the last five steps of the approach run than the LSLA group in men.ConclusionsCoaches should implement exercises targeting SLA in long and triple jump training exercises to improve performance.
The efficiency of integrated and segregated physical education classes for secondary school students with physical and mental disabilities and poor fitness
Authors: Bodnar Ivanna , Prystupa Evhen 
Published: April 2016

PurposeThe aim of our investigation was to establish the efficiency of the integrated physical education classes for schoolchildren with minor deviations in health.MethodsSociological (questionnaire), biomedical, pedagogical (testing), psycho-diagnostic, and mathematical statistics. Pupils (<i>N</i> = 1417) of secondary school (both sexes, 5<sup>th</sup>−9<sup>th</sup> forms, i.e. aged 10−15) were examined.ResultsThe characteristics of physical and mental development in the process of integrated physical education proved to be significantly better for different sex and age groups. Analysis of physical fitness characteristics shows that integrated physical education classes turned out to be more effective for schoolchildren with minor health deviations than for apparently healthy students. Integrated physical education classes are more effective for students’ dexterity than for their power endurance. Integrated physical education classes are more effective for female students than for male ones; for schoolchildren with minor health deviations in older age groups (7<sup>th</sup>−9<sup>th</sup> forms) than for younger schoolchildren. Those schoolchildren who attended integrated physical education classes expressed significantly better attitudes to physical education than their peers who studied in a segregated environment.ConclusionsIntegrated physical education classes are a more progressive and effective form of physical education in terms of their beneficial effect upon the physical and mental development of secondary school children with minor health distortions.
Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae
Authors: Jain Suvi , Sugawara Neal , Mehta Anuja , Ryu Taehyun , Haber James E 
Published: April 2016

Abstract
We have previously shown that a recombination execution checkpoint (REC) regulates the choice of the homologous recombination pathway used to repair a given DNA double-strand break (DSB) based on the homology status of the DSB ends. If the two DSB ends are synapsed with closely-positioned and correctly-oriented homologous donors, repair proceeds rapidly by the gene conversion (GC) pathway. If, however, homology to only one of the ends is present, or if homologies to the two ends are situated far away from each other or in the wrong orientation, REC blocks the rapid initiation of new DNA synthesis from the synapsed end(s) and repair is carried out by the break-induced replication (BIR) machinery after a long pause. Here we report that the simultaneous deletion of two 3′→5′ helicases, Sgs1 and Mph1, largely abolishes the REC-mediated lag normally observed during the repair of large gaps and BIR substrates, which now get repaired nearly as rapidly and efficiently as GC substrates. Deletion of SGS1 and MPH1 also produces a nearly additive increase in the efficiency of both BIR and long gap repair; this increase is epistatic to that seen upon Rad51 overexpression. However, Rad51 overexpression fails to mimic the acceleration in repair kinetics that is produced by sgs1Δ mph1Δ double deletion.
Relationship between the nutritional status of banana plants and black sigatoka severity in the Magdalena region of Colombia
Authors: Aguirre Sonia Esperanza , Piraneque Nelson Virgilio , Rodríguez Barrios Javier 
Published: April 2016

The association between the severity (average percentage of infection-API) by Mycosphaerella fijiensisMorelet and the plant nutrient content in the banana growing zone of the department of Magdalena (Colombia) was established. Between 2011 and 2012, the foliar contents of N, P, K, Ca, Mg, Na, S, Cu, Fe, B, Zn, and Mn were determined in sectors with high, medium, and low incidences in order to establish their relationships with the API. Severity was determined with the Stover and Dickson methodology, modified by Gauhl for bananas, in order to obtain sanitary information for the zone. With the obtained data, a correlation analysis was completed and the ordination technique was utilized to establish the relationships between farms and variables using an Euclidean distance. The differences between the farms and years were estimated with a two way analysis of variance with permutations and a canonical discrimination analysis in order to differentiate the farms using the measured foliar variables. The results highlighted the importance of the appropriate and balanced management of site-specific nutritional plans for the management of black sigatoka.
Structural, hardness and tribological behavior of TiAlNO coatings prepared by sputtering
Authors: García González L. , Vásquez García S. R. , Zamora Peredo L. , López Velázquez A. , Domratcheva Lvova L. , Flores Ramírez N. , Garnica Romo M. G. , Hernández Quiroz T. , Hernández Torres J. 
Published: April 2016

ABSTRACTAluminum titanium oxynitride (TiAlNO) coatings were deposited on 316 steel substrates by the sputtering technique, varying the nitrogen flow from 2.5, 5, 7.5 to 10 sccm, and maintaining constant at 12 sccm the flow argon gas. We used targets of titanium and alumina with 99.995% purity. The hardness and tribological analyses were determined by Vickers microhardness and tribology (tribometer pin-disc), respectively. The results show that the coating with a nitrogen flow of 10 sccm had the lowest volumetric wear (2.047738693 mm3) and the maximum value of hardness (11.2 GPa). Analysis of X-ray diffraction evidenced the presence of three crystalline phases: Ti2N, Al2O3 and TiO2. It can be observed that by increasing the nitrogen flow, the portion of semi-Ti2N phase increases, Al2O3 decreases and TiO2 remains almost constant, and also producing a change in crystallographic orientation with reference to the Ti2N phase. Crystal grain sizes were estimated by X-ray diffraction Fourier line profile analysis using Warren–Averbach method. This analysis showed a grain size between 5 and 15 nm. Raman spectroscopy results show the presence of the TiO2 phase which corroborated the X-ray diffraction results.
Inquiries into commissions of inquiry into police
Authors: Van der Spuy Elrena 
Published: April 2016

Commissions of inquiry into police have a long and chequered history - both internationally and locally. In this special edition, our focus falls on the two most recent commissions of inquiry into police in South Africa - parochially known as the Marikana and Khayelitsha commissions. The deliberations of the two commissions have attracted much attention. Media coverage has at times opted for the sensational. However, a steady stream of more thoughtful commentaries, crafted by investigative journalists and policing experts, has also seen the light. The archival footprints of both commissions have been enormous. Thousands of pages of information on a wide range of administrative, operational and policy matters of relevance to police and policing have been produced. The online storage of expert testimonies, witness statements, and transcriptions of cross-examinations has further enhanced access to both primary and secondary material. Amid this abundance of data it would seem that those interested in the trials and tribulations of policing the post-colony can do no better than to capitalise on the opportunity for critical reflection and substantive analysis. In this special edition of the South African Crime Quarterly we take up the challenge for reflection.
PEMBELAJARAN KONTRUKTIFISTIK DENGAN PENDEKATAN CTL PADA TEORI BELAJAR BERMAIN DIENES
Authors: Rahman Hairur 
Published: April 2016

<p>In order to improve the students’ mastering at elementary schools, it is important to note that the teaching strategies should not be only focused on “how to teach”, however the teaching should be also focused on “how to stimulate” and “how to learn”. In this context, the contextual teaching and learning approach (CTL) is considered as the new approach, particularly for math subject, which has been applied in Indonesia. For this purpose, this article offers the concept teaching model by Dienes theory using CTL approach.</p><p>Kata Kunci: CTL, Konstruktivisme, Teori Belajar Bermain Dienes. <br /><br /></p>
URGENSI LINGKUNGAN DALAM PROSES PENDIDIKAN ANAK
Authors: Yaqien Nurul 
Published: April 2016

In the Islamic school process, environment-role is affecting to the education accomplishment, either in Islamic school or not. Education field out of Islamic school such as; home-schooling and society-schooling. If these could work each other propitiously, so the result would be filled the bill by government, people, and religion. Otherwise, if Islamic education is only focused on classroom-teaching and paying no regard to society education. Thus, very hard to attain intended outcome. Furthermore, if Islamic education does not care about family setting so will occur teaching learning process-failure especially Islamic religion subject. Therefore, in the carrying out of education in Islamic school, the importance of environment setting in education needs serious consideration to figure ideal human by government, people, and religion. In order that Islamic education is not gone over. <br /> Keyword: Enviroment, Islamic-Education <br /><br />
TREND REFORMASI PENDIDIKAN DI AUSTRALIA
Authors: Esha Muhammad In’am 
Published: April 2016

Change is an important term in this era. Change is a phenomenon that able to seen in various aspects such as politic, economic, social, and education. Political reform or education reform that make a trend in the world, actually speak about change. This paper examines education reform in Australia. This study shows several aspect on that phenomenon. First, education reform in Australia influenced by spreading of neoliberalism trend in the world with a famous term: new public management paradigm. As member of OECD, Australia must develop and modernize its education as well as in New Zealand, England, Japan, and USA. Second, education reform needed for competing in globalization era. Relation of education with increasing human resources is an important factor that encourage education reform. Third, education reform in Australia include two aspect: managerial aspect and content of education (curriculum). <br />Keyword: Reform, Education, New Public Management.<br /><br />