bluuebunny/crossref_metadata_2025_split huggingface dataset:
HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma
Authors: Tsuji Shoutaro , Washimi Kota , Kageyama Taihei , Yamashita Makiko , Yoshihara Mitsuyo , Matsuura Rieko , Yokose Tomoyuki , Kameda Yoichi , Hayashi Hiroyuki , Morohoshi Takao , Tsuura Yukio , Yusa Toshikazu , Sato Takashi , Togayachi Akira , Narimatsu Hisashi , Nagasaki Toshinori , Nakamoto Kotaro , Moriwaki Yasuhiro , Misawa Hidemi , Hiroshima Kenzo , Miyagi Yohei , Imai Kohzoh 
Published: March 2017

AbstractThe absence of highly specific markers for malignant mesothelioma (MM) has served an obstacle for its diagnosis and development of molecular-targeting therapy against MM. Here, we show that a novel mucin-like membrane protein, sialylated protein HEG homolog 1 (HEG1), is a highly specific marker for MM. A monoclonal antibody against sialylated HEG1, SKM9-2, can detect even sarcomatoid and desmoplastic MM. The specificity and sensitivity of SKM9-2 to MM reached 99% and 92%, respectively; this antibody did not react with normal tissues. This accurate discrimination by SKM9-2 was due to the recognition of a sialylated O-linked glycan with HEG1 peptide. We also found that gene silencing of HEG1 significantly suppressed the survival and proliferation of mesothelioma cells; this result suggests that HEG1 may be a worthwhile target for function-inhibition drugs. Taken together, our results indicate that sialylated HEG1 may be useful as a diagnostic and therapeutic target for MM.
Identification of two p53 isoforms from Litopenaeus vannamei and their interaction with NF-κB to induce distinct immune response
Authors: Li Haoyang , Wang Sheng , Chen Yonggui , Lǚ Kai , Yin Bin , Li Sedong , He Jianguo , Li Chaozheng 
Published: March 2017

Abstractp53 is a transcription factor with capability of regulating diverse NF-κB dependent biological progresses such as inflammation and host defense, but the actual mechanism remains unrevealed. Herein, we firstly identified two novel alternatively spliced isoforms of p53 from Litopenaeus vannamei (LvΔNp53 and the full-length of p53, LvFLp53). We then established that the two p53 isoforms exerted opposite effects on regulating NF-κB induced antimicrobial peptides (AMPs) and white spot syndrome virus (WSSV) immediate-early (IE) genes expression, suggesting there could be a crosstalk between p53 and NF-κB pathways. Of note, both of the two p53 isoforms could interact directly with LvDorsal, a shrimp homolog of NF-κB. In addition, the activation of NF-κB mediated by LvDorsal was provoked by LvΔNp53 but suppressed by LvFLp53, and the increased NF-κB activity conferred by LvΔNp53 can be attenuated by LvFLp53. Furthermore, silencing of LvFLp53 in shrimp caused higher mortalities and virus loads under WSSV infection, whereas LvΔNp53-knockdown shrimps exhibited an opposed RNAi phenotype. Taken together, these findings present here provided some novel insight into different roles of shrimp p53 isoforms in immune response, and some information for us to understand the regulatory crosstalk between p53 pathway and NF-κB pathway in invertebrates.
Herbicidal properties of antimalarial drugs
Authors: Corral Maxime G. , Leroux Julie , Stubbs Keith A. , Mylne Joshua S. 
Published: March 2017

AbstractThe evolutionary relationship between plants and the malarial parasite Plasmodium falciparum is well established and underscored by the P. falciparum apicoplast, an essential chloroplast-like organelle. As a result of this relationship, studies have demonstrated that herbicides active against plants are also active against P. falciparum and thus could act as antimalarial drug leads. Here we show the converse is also true; many antimalarial compounds developed for human use are highly herbicidal. We found that human antimalarial drugs (e.g. sulfadiazine, sulfadoxine, pyrimethamine, cycloguanil) were lethal to the model plant Arabidopsis thaliana at similar concentrations to market herbicides glufosinate and glyphosate. Furthermore, the physicochemical properties of these herbicidal antimalarial compounds were similar to commercially used herbicides. The implications of this finding that many antimalarial compounds are herbicidal proffers two novel applications: (i) using the genetically tractable A. thaliana to reveal mode-of-action for understudied antimalarial drugs, and (ii) co-opting antimalarial compounds as a new source for much needed herbicide lead molecules.
Polymorphisms of RAD51B are associated with rheumatoid arthritis and erosion in rheumatoid arthritis patients
Authors: Zhi Liqiang , Yao Shuxin , Ma Wenlong , Zhang Weijie , Chen Honggan , Li Meng , Ma Jianbing 
Published: March 2017

AbstractRheumatoid arthritis (RA) is a common, chronic autoimmune disease affecting 0.5–1.0% of adults worldwide, including approximately 4.5–5.0 million patients in China. The genetic etiology and pathogenesis of RA have not yet been fully elucidated. Recently, one new RA susceptibility gene (RAD51B) has been identified in Korean and European populations. In this study, we designed a two-stage case-control study to further assess the relationship of common variants in the RAD51B gene with increased risk of RA in a total of 965 RA patients and 2,511 unrelated healthy controls of Han Chinese ancestry. We successfully identified a common variant, rs911263, as being significantly associated with the disease status of RA (P = 4.8 × 10−5, OR = 0.64). In addition, this SNP was shown to be related to erosion, a clinical assessment of disease severity in RA (P = 2.89 × 10−5, OR = 0.52). These findings shed light on the role of RAD51B in the onset and severity of RA. More research in the future is needed to clarify the underlying functional link between rs911263 and the disease.
Hearing Loss in the Elderly: Is the Hearing Handicap Inventory for the Elderly - Screening Version Effective in Diagnosis When Compared to the Audiometric Test?
Authors: Servidoni Alexandre , Conterno Lucieni 
Published: March 2017

Introduction Hearing losses inherent to the natural process of aging represent today a major public health issue, despite the little attention that their adequate care still receives. Early recognition and proper management of these shortcomings can significantly improve hearing, as well as the patient's general quality of life, reducing the overall impact of this important and prevalent condition of the aging process.
Objective The aim of this research was to evaluate the accuracy of the Hearing Handicap Inventory for the Elderly - Screening Version (HHIE-S) in the diagnosis of hearing loss in the elderly when compared with the audiometric test.
Methods Through a cross-sectional study, our target population was composed of 138 individuals, aged over 60 and with any otorhinolaryngological complaints, recruited at the Clinic of Otorhinolaryngology and Speech Therapy of the Faculdade de Medicina de Marília (Famema), in the city of Marília, SP, Brazil. Patients already in the process of auditory rehabilitation were excluded, as well as those who did not demonstrate the minimum level of oral understanding necessary to allow the interview.
Results The prevalence of hearing loss according to the questionnaire was of 76.1%, while audiometry showed 79.7%. We found the diagnostic accuracy of the instrument to be of 86.2%, with a sensitivity of 89.1% and a specificity of 75.0%, regardless of gender.
Conclusion Thereby, we conclude that the standardized questionnaire under rating is suitable for the screening of hearing loss in the elderly, given its high accuracy and user-friendly quality.
The impact of diet liberalization on bowel preparation for colonoscopy
Authors: Walter James , Francis Gloria , Matro Rebecca , Kedika Ramalinga , Grosso Rachael , Keith Scott , Kastenberg David 
Published: March 2017

Abstract
Background and study aims Dietary restrictions are integral to colonoscopy preparation and impact patient satisfaction. Utilizing split-dose, lower-volume polyethylene glycol 3350-electrolyte solution (PEG-ELS), this study compared colon preparation adequacy of a low-residue diet to clear liquids using a validated grading scale.
Patients and methods This was a prospective, randomized, single-blinded, single-center non-inferiority study evaluating diet the day prior to outpatient colonoscopy. Subjects were randomized to a Low-Residue diet for breakfast and lunch, or Clears only. All subjects received split dose PEG-ELS. The primary endpoint was preparation adequacy using the Boston Bowel Preparation Scale (BBPS), with adequate defined as a score > 5. Secondary endpoints included mean BBPS scores for the entire colon and individual segments, satisfaction, adverse events, polyp and adenoma detection rates, and impact on sleep and daily activities.
Results Final analysis included 140 subjects, 72 assigned to Clears and 68 to Low-Residue. The Low-Residue diet was non-inferior to Clears (risk difference = – 5.08 %, P = 0.04) after adjusting for age. Mean colon cleansing scores were not significantly different overall and for individual colonic segments. Satisfaction with the Low-Residue diet was significantly greater (P = 0.01). The adenoma detection rate was not statistically significantly different between study groups, but the number of adenomas detected was significantly greater with Clears (P = 0.01). Adverse events and impact on sleep and activities did not differ significantly between diet arms.
Conclusions A low-residue diet for breakfast and lunch the day prior to colonoscopy was non-inferior to clear liquids alone for achieving adequate colon cleansing when using split dose PEG-ELS.
The role of the argument by example in legislative debates of the European Parliament
Authors: Garssen Bart 
Published: March 2017

AbstractThis paper focuses on the role of the argument by example in the argumentation put forward by Members of the European Parliament. The argumentative patterns that come into being in legislative debates in the European Parliament depend for the most part on the problem-solving argumentation that is put forward in the opening speech by the rapporteur of the parliamentary committee report. Complex problem-solving argumentation consists of a premise stating that there is a problem (the problem statement) and a premise stating that the proposed legislation will solve the problem (the causal statement). In their contributions, MEPs who are in favor of the proposal will either defend the problem statement or the causal statement. This paper examines how an argument by example is used in order to defend the problem statement. The argument by example can be used to defend the existential presupposition as well as the normative presupposition in the problem-statement.
Argumentative patterns in the European Union’s directives
Authors: Andone Corina , Coman-Kund Florin 
Published: March 2017

AbstractThis paper provides an account of the arguments advanced by the European Union (EU) legislator in the preamble of directives adopted for harmonization in the internal market, and assesses them as to their potential at convincing the Member States to implement the directive at issue. We show what directives should argue for and how they do so in practice, by focussing in particular on Directive 2011/83/EU on consumer rights. Furthermore, this contribution moves beyond a purely academic discussion by linking the theoretical-normative framework advanced to the Court of Justice of the European Union’s approach to assessing the preambles of EU directives in the context of the ‘check’ on the duty to state reasons under Article 296 Treaty for the of the Functioning of the European Union (TFEU). Our analysis unveils a legislative practice in which the obligation to give reasons is not discharged adequately from an argumentative perspective, and which remains generally unsanctioned due to the rather light and flexible test used by CJEU under Article 296 TFEU.
Some clarification of the impacts of grassland intensification on food production, nitrogen release, greenhouse gas emissions and carbon sequestration: using the example of New Zealand.
Authors: Parsons A. J. , Thornley J. H. M. , Rasmussen S. , Rowarth J. S. 
Published: March 2017

Abstract
We used an established, process-based model of the dynamics of carbon (C) and nitrogen (N) cycling between plants, soils and animals in grazed temperate pastures to clarify expectations of how some major components of intensification affect the outcomes (short-term and long-term) of alternative systems for food production and environmental impact We use the example of New Zealand, due to its clear recent history of intensification, the level of concern and hence nature of research undertaken there. A transition from low-input drystock, to dairy (lactation) systems with higher N inputs in fertilizer, and/or C and N inputs in supplements, reveals how at the same intermediate N input, food yield (per ha) can be doubled, while environmental N release halved, with minimal impact on C sequestration. We stress the sources of sustained changes in N release (e.g. nitrate/nitrous oxide) are altered inputs (fertilizer/supplements) and less so animal numbers in response to these. Much of the increased efficiency is due to 'improved' N partitioning in lactating (cf. dry) animals. A reversion to dry-stock (or 'de-stocking') therefore offers greater environmental challenge, unless N inputs decline accordingly. Responses to supplements (being a source of C) re-inforce how the driving limitation to the grazed ecosystem is C capture per ha, and we highlight the need for a renewed focus on fundamental research on plant C uptake per ha per unit of N input We offer a graphical method for visualizing the outcomes of options, and their trade-offs, with implications for policy and future research direction.
Animal agriculture as a contributor to the global challenge of antibiotic resistance.
Authors: Littier H. M. , Chambers L. R. , Knowlton K. F. 
Published: March 2017

Abstract
Antimicrobial resistance is an issue of global concern and the quantity of antibiotics used in animal agriculture make it an obvious potential contributor. Subtherapeutic use of antibiotics has likely been the largest contributor to this phenomenon, due to the selection pressure exerted over extended periods of time in the livestock microbiome. The observation of antibiotic resistance in the microbiome (gut, faeces, meat, milk, etc.) of food-producing animals is becoming increasingly common. This paper reviews the literature regarding the contribution of animal agriculture to the global challenge of antimicrobial resistance with a focus on manure, soil and water as reservoirs of resistance. The question of whether reducing or eliminating the use of antibiotics in animal agriculture would be sufficient, effective, or appropriate is addressed, and research priorities are identified.
Improving Catch Utilization in the U.S. West Coast Groundfish Bottom Trawl Fishery: an Evaluation of T90-Mesh and Diamond-Mesh Cod Ends
Authors: Lomeli Mark J. M. , Hamel Owen S. , Wakefield W. Waldo , Erickson Daniel L. 
Published: March 2017

Abstract
The limited-entry bottom trawl fishery for groundfish along the U.S. West Coast operates under a catch share program, which is implemented with the intention of improving the economic efficiency of the fishery, maximizing fishing opportunities, and minimizing bycatch. However, stocks with low harvest guidelines have limited the ability of fishermen to maximize their catch of more abundant stocks. Size-selection characteristics of 114-mm and 140-mm T90-mesh cod ends and the traditional 114-mm diamond-mesh cod end were examined by using the covered cod end method. Selection curves and mean L50 values (length at which fish had a 50% probability of being retained) were estimated for two flatfish species (Rex Sole Glyptocephalus zachirus and Dover Sole Microstomus pacificus) and two roundfish species (Shortspine Thornyhead Sebastolobus alascanus and Sablefish Anoplopoma fimbria). Mean L50 values were smaller for flatfishes but larger for roundfishes in the 114-mm T90 cod end compared to the diamond-mesh cod end. For Rex Sole, Dover Sole, and Shortspine Thornyheads, selectivities of the 140-mm T90 cod end were significantly different from those of the other cod ends; the 140-mm T90 cod end was most effective at reducing the catch of smaller-sized fishes but with a considerable loss of larger-sized marketable fishes. Findings suggest that T90 cod ends have potential to improve catch utilization in this multispecies fishery.
CCM.FF-K3.2011: Final report for the CIPM key comparison of air speed, 0.5 m/s to 40 m/s
Authors: Müller Harald , Caré Isabelle , Lucas Peter , Pachinger Dietmar , Kurihara Noboru , Lishui Cui , Su Chun-Min , Shinder Iosif , Spazzini Pier Giorgio 
Published: March 2017

The CCM.FF-K3.2011 comparison was organized for the purpose of determination of the degree of equivalence of the national standards for air speed over the range 0.5 m/s to 40 m/s. An ultrasonic anemometer and a Laser Doppler anemometer were used as transfer standards. Nine laboratories from three RMOs participated between July 2013 and July 2015—EURAMET: PTB, Germany; LNE-CETIAT, France; INRIM, Italy; VSL, The Netherlands; E+E, Austria; SIM: NIST, USA; APMP: NMIJ/AIST, Japan; NIM, China; CMS/ITRI, Chinese Taipei. The measurements were provided at ambient conditions. All results of independent participants were used in the determination of the key comparison reference value (KCRV) and the uncertainty of the KCRV. The reference value was determined at each air speed separately following `procedure A' presented by M G Cox. The degree of equivalence with the KCRV was calculated for each air speed and laboratory. Almost all reported results were consistent with the KCRV.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
International comparison CCQM-K84—carbon monoxide in synthetic air at ambient level
Authors: Lee Jeongsoon , Moon Dongmin , Lee Jinbok , Lim Jeongsik , Hall Brad , Novelli Paul , Brewer Paul J , Miller Michael , Murugun Arul , Minarro Marta Doval , Qiao Han , Shuguo Hu , Konopelko L A , Kustikov Y A , Kolobova A V , Pankratov V V , Wasserman I I , Zav'yalov S V , Efremova O V , Pavlov M V , Mitchell Gerald , Guenther Frank , Walden Jari , Aoki Nobuyuki , Shimosaka Takuya , Tatiana Mace , Lagler F , Borowiak Annette 
Published: March 2017

Carbon monoxide (CO) is reported to mainly be emitted from industries, transportation, and burnings for various usages. Its atmospheric lifetime varies from weeks to months, depending on the mixing ratio of the highly reactive hydroxyl radical. Even though the ambient level of CO varies as a function of regional sources, the mixing ratio ranges from 30 nmol/mol to 300 nmol/mol at the marine boundary layers and from 100 nmol/mol to more than 500 nmol/mol in urban areas(1). In order to study temporal trends and regional variations of the level of CO, the National Oceanic & Atmospheric Administration/Earth System Research Laboratory-Global Monitoring Division (NOAA/ESRL-GMD(2)) has played a key role as the designated Central Calibration Laboratory (CCL) within the frame of the World Meteorological Organization (WMO) Global Atmosphere Watch (GAW) program. NOAA/ESRL-GMD provides natural air standards, analyzed for CO, to WMO GAW participants. Since the structure of WMO traceability chain appears hierarchical and explicit all over the world, WMO intends to improve the CO measurement compatibility to up to 2 ppb (in case of extensive compatibility goal: 5 ppb, GAW report No. 213(3)) in order to ensure compatibility through the GAW network. Nevertheless, accurate measurement of CO at an ambient level has proven to be difficult due to the lack of stability in cylinders. For these reasons, it is necessary that measured results are compared among the values assigned by various NMIs.
This key comparison was initially proposed to be aimed at a CO/N2 standard in the 2010 CCQM meeting by KRISS. With participation of FMI, NOAA, and Empa, a modified scheme of CO/air standards was developed for the purpose of atmospheric observations and co-operative support to WMO/GAW activities. Therefore, the purpose of the comparison is to support the measurement capability of CO at an ambient level of 350 nmol/mol. Further, this key comparison is expected to contribute to the establishment of traceability to a single scale of CO between NMIs by means of harmonizing the results from different national standards. The Empa result lies in a different report.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Coronary Tortuosity: A Clue to the Diagnosis of Fibromuscular Dysplasia?
Authors: van Twist Daan J. L. , de Leeuw Peter W. , Kroon Abraham A. 
Published: March 2017

Abstract

BACKGROUND
Fibromuscular dysplasia (FMD) is a systemic, nonatherosclerotic, noninflammatory vasculopathy that is often overlooked by clinicians. Clinical clues could help in selecting patients for further evaluation for the presence of FMD. Recently, it was observed that tortuosity of the coronary arteries is often present in patients with FMD-related abnormalities of the coronary artery. Therefore, we wondered if the presence of coronary tortuosity might provide a clinical clue to the diagnosis of extracoronary FMD.

CASES
We describe 5 cases of FMD in whom diagnostic studies for FMD were initiated because of the presence of coronary tortuosity. FMD was found in all 5 patients in the renal and/or cervical arteries.

CONCLUSIONS
Our 5 cases suggest that exertional chest pain in the presence of coronary tortuosity may be helpful in selecting patients for further evaluation for the presence of FMD. Further research should focus on the prevalence of FMD among patients with coronary tortuosity and whether the presence of additional clinical clues (such as the presence of hypertension at young age or pulsatile tinnitus) next to coronary tortuosity can predict the risk for FMD in individual patients.