bluuebunny/crossref_metadata_2025_split huggingface dataset:
Object words modulate the activity of the mirror neuron system during action imitation
Authors: Wu Haiyan , Tang Honghong , Ge Yue , Yang Suyong , Mai Xiaoqin , Luo Yue‐Jia , Liu Chao 
Published: September 2017

AbstractBackgroundAlthough research has demonstrated that the mirror neuron system (MNS) plays a crucial role in both action imitation and action‐related semantic processing, whether action‐related words can inversely modulate the MNS activity remains unclear.MethodsHere, three types of task‐irrelevant words (body parts, verbs, and manufactured objects) were presented to examine the modulation effect of these words on the MNS activity during action observation and imitation. Twenty‐two participants were recruited for the fMRI scanning and remaining data from 19 subjects were reported here.ResultsBrain activity results showed that word types elicited different modulation effects over nodes of the MNS (i.e., the right inferior frontal gyrus, premotor cortex, inferior parietal lobule, and STS), especially during the imitation stage. Compared with other word conditions, action imitation following manufactured objects words induced stronger activation in these brain regions during the imitation stage. These results were consistent in both task‐dependent and ‐independent ROI analysis.ConclusionOur findings thus provide evidence for the unique effect of object words on the MNS during imitation of action, which may also confirm the key role of goal inference in action imitation.
Lentivirus‐mediated disintegrin and metalloproteinase 17 RNA interference reversed the acquired resistance to gefitinib in lung adenocarcinoma cells in vitro
Authors: Li Ya‐Qing , Liu Yuan‐Shun , Ying Xi‐Wang , Zhou Hong‐Bin , Wang Zhehua , Wu Sheng‐Chang , Yan Jian‐Ping , Jing Yu‐Ting , Yang Yang 
Published: September 2017

Objective: The aim of the study is to evaluate the effects of silencing a disintegrin and metalloproteinase 17 (ADAM17) gene expression by lentivirus‐mediated RNA interference (RNAi) in the gefitinib‐resistant lung adenocarcinoma cells, and then to explore whether the recombinant lentivirus mediated ADAM17 RNAi reversed the acquired resistance of lung adenocarcinoma to gefitinib in vitro.Methods: The gefitinib‐resistant RPC‐9 cells were established and the mutations of EGFR were detected by gene sequencing. The ADAM17 shRNA expression vectors were constructed and packaged to recombinant lentivirus. The cell proliferation viability was detected by MTT, and cellular apotosis was analyzed by flow cytometry assay. The expression levels of ADAM17, EGFR and the phosphorylated EGFR were respectively detected by reverse transcription polymerase chain reaction and western blot. TGF‐α production in the supernatant was detected by enzyme‐linked immunosorbent assay.Results: The gefitinib‐resistant RPC‐9 cells in which mutated EGFR (exon 20) carried 790T > T/M mutation were established. When the concentrations of gefitinib were less than 10μmol/L, there were no significant changes in the apoptosis and cellular proliferation of RPC‐9 with the dose‐escalation of gefitinib. The cell proliferation viability of RPC‐9 was significantly decreased by lentivirus mediated ADAM17 RNAi (P < 0.05). Gefitinib did not inhibit ADAM17 expression in both the gefitinib‐sensitive PC‐9 and gefitinib‐resistant RPC‐9 cells (P > 0.05). Gefitinib had no significant effects on TGF alpha production in the supernatants (P > 0.05). Gefitinib did not inhibit EGFR expression in gefitinib‐sensitive PC‐9 and gefitinib‐resistant RPC‐9 cells (P > 0.05). The phosphorylation of EGFR in gefitinib‐sensitive PC‐9 cells was significantly inhibited by gefitinib (P < 0.05), but that in gefitinib‐resistant RPC‐9 could not be inhibited by gefitinib (P > 0.05). Lentivirus mediated ADAM17 RNAi significantly inhibited the mRNA and protein expression of ADAM17 in gefitinib‐resistant RPC‐9 cells (P < 0.05), as well as TGF alpha production in the supernatants (P < 0.05). Also, the phosphorylation of EGFR was significantly reduced in gefitinib‐resistant RPC‐9 cells by lentivirus mediated ADAM17 RNAi (P < 0.05); however, the mRNA and protein expression of EGFR could not be inhibited.Conclusion: Lentivirus mediated ADAM17 RNAi may reverse the acquired resistance of lung adenocarcinoma to gefitinib via inhibiting the upstream of EGFR signal pathway, which may provide a new therapeutic target to solve the acquired resistance to EGFR tyrosine kinase inhibitors in lung adenocarcinoma. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 34:196–205, 2018
Catheter ablation of typical atrial flutter with the superior approach in a patient with inferior vena cava interruption
Authors: Traykov Vassil , Marchov Daniel , Kirova Galina , Gelev Valeri 
Published: September 2017

Key Clinical MessageInferior vena cava (IVC) interruption is a rare condition that might pose difficulties during typical flutter ablation. When azygos vein continuation is present ablation via the femoral route could be performed. In the absence of azygos vein continuation, typical atrial flutter ablation via a superior approach from the SVC is feasible.
The extra‐anatomical jump graft reconstruction of the right hepatic artery after resection of a biliary tract malignancy with a common hepatic artery aneurysm: a case report
Authors: Kakizaki Yuta , Miyagi Shigehito , Hara Yasuyuki , Fujio Atsushi , Miyazawa Koji , Maida Kai , Kashiwadate Toshiaki , Tokodai Kazuaki , Nakanishi Chikashi , Kamei Takashi , Unno Michiaki 
Published: September 2017

Key Clinical MessagePerforming resection of a biliary tract malignancy with a hepatic artery aneurysm is very challenging. Resection of the extrahepatic bile duct and extra‐anatomical reconstruction can be successfully performed using free radial artery autografts from the aorta to the right hepatic artery. Hepatic artery thrombosis can be prevented with intimal preservation.
Bony spicules trapped in peri‐implant soft tissue: a common unrecognized finding
Authors: Sampatanukul Teeratida , Serichetaphongse Pravej , Sampatanukul Pichet , Pimkhaokham Atiphan 
Published: September 2017

Key Clinical MessageAccording to the study, there were unexpected tiny bone spicules being inspected in peri‐implant soft tissue. These displaced autogenous bone chips were probably presented when preparing implant sites. The displaced bone spicules seemed not induced significant inflammatory reactions; on contrary, defects of specimens and dissolving bone spicules pictures were demonstrated.
Effects of duloxetine on pain and walking distance in neuropathic pain models via modulation of the spinal monoamine system
Authors: Minami K. , Tamano R. , Kasai E. , Oyama H. , Hasegawa M. , Shinohara S. , Asaki T. 
Published: September 2017

AbstractBackgroundApproximately 40% of patients with chronic low back pain have a neuropathic component. In this study, we assessed the effects of analgesics on tactile hypersensitivity and walking distance in the rat cauda equina compression (CEC) model of neuropathic low back pain.MethodsThe effects of analgesics on tactile hypersensitivity were examined using the von Frey test in CEC and partial sciatic nerve ligation (pSNL) models. Effects on walking distance were assessed using a treadmill test. Levels of α2δ1 subunit and ATF‐3 mRNA in dorsal‐root ganglion (DRG) neurons and those of α2δ1 subunit protein in the spinal cord were determined using quantitative RT‐PCR and western blotting, respectively. Histological features were assessed using immunohistological methods.ResultsHistological changes indicating nerve damage (increase in ATF‐3 mRNA, decrease in NF‐200 and an increase in CD68 immunoreactivity) were observed in the CEC model. Duloxetine had analgesic effects in both models and improved walking distance in the CEC model. Pregabalin had analgesic effects in both models; however, the effect was weaker in the CEC model than in the pSNL model. α2δ1 subunit expression in DRG neurons and in the spinal cord was unchanged in the CEC model, but significantly increased in the pSNL model. Indomethacin had no analgesic effect in either model. Intrathecal yohimbine inhibited the effects of duloxetine with significant effects on depression.ConclusionsThese findings suggest that the analgesic effects of duloxetine are mainly mediated by the spinal monoamine system, independent of the antidepressant effects of this agent.SignificanceThe findings of this study suggest that duloxetine may be an effective treatment of broad neuropathic pain states, including neuropathic low back pain. The analgesic effects of duloxetine might be mediated by alterations of the descending pain modulatory pathways in the spinal cord, independent of the antidepressant effects.
Pencil‐drawn Paper‐based Non‐invasive and Wearable Capacitive Respiration Sensor
Authors: Kanaparthi Srinivasulu 
Published: September 2017

AbstractThis paper describes a fully‐drawn pencil‐on‐paper based low‐cost capacitive sensor for non‐invasive respiration monitoring. The sensor utilizes the hygroscopic character of the paper to measure the breathing rate and pattern. The adsorption and desorption of water molecules on paper during inhalation and exhalation results in variation in its dielectric constant. This change in dielectric constant during respiration reflects the change in capacitance of the sensor. By interfacing the sensor with the microcontroller, the capacitance data was acquired and transferred to a smartphone through Bluetooth communication. Being a low cost, wearable, non‐invasive and disposable sensor, it holds tremendous potential in healthcare technology and can be commercialized into a viable product for easy‐to‐use diagnostic purpose.
Enantioseparation of nornicotine in tobacco by ultraperformance convergence chromatography with tandem mass spectrometry
Authors: Deng Huimin , Wang Ying , Bian Zhaoyang , Liu Shanshan , Fan Ziyan , Li Zhonghao , Yang Fei , Tang Gangling 
Published: September 2017

AbstractNornicotine, an alkaloid constituent of tobacco, is a precursor to the carcinogen N‐nitrosonornicotine that is produced during the curing and processing of tobacco. Accumulating evidence reveals that nornicotine enantiomers have different neurochemical and behavioral effects. In the present study, an accurate and rapid method was developed for the enantioseparation of (R)‐(+)‐nornicotine and (S)‐(−)‐nornicotine enantiomers in tobacco by ultra‐performance convergence chromatography with tandem mass spectrometry. Chromatographic conditions were investigated to achieve the optimal resolution of two enantiomers. Results indicated that (R)‐(+)‐nornicotine and (S)‐(−)‐nornicotine could be separated within 5 min when ammonium hydroxide was added into the cosolvent, and the best resolution (Rs = 4.76) was achieved on a immobilized cellulose tris‐(3,5‐dichlorophenylcarbamate) chiral stationary phase. The proposed method was validated and was finally applied to analyze the compositions of (R)‐(+)‐nornicotine and (S)‐(−)‐nornicotine in three typical types of tobaccos (flue‐cured, burley, and oriental). It was found that, enantiomer fraction of nornicotine (the proportion of (S)‐(−)‐nornicotine in the nornicotine pool) in burley tobacco samples was relatively high and constant compared with flue‐cured and oriental tobaccos. The effective and rapid enantioseparation of nornicotine may help the understanding of alkaloid metabolites in different tobacco varieties and may also benefit pharmacological studies of alkaloid enantiomers.
Weighted Gene Co‐Expression Network Analysis Identifies Gender Specific Modules and Hub Genes Related to Metabolism and Inflammation in Response to an Acute Lipid Challenge
Authors: Fatima Attia , Connaughton Ruth M. , Weiser Anna , Murphy Aoife M. , O'Grada Colm , Ryan Miriam , Brennan Lorraine , O'Gaora Peadar , Roche Helen M. 
Published: September 2017

ScopeInflammation is characteristic of diet‐related diseases including obesity and type 2 diabetes (T2D). However, biomarkers of inflammation that reflect the early stage metabolic derangements are not optimally sensitive. Lipid challenges elicit postprandial inflammatory and metabolic responses. Gender‐specific transcriptomic networks of the peripheral blood mononuclear cell (PBMC) were constructed in response to a lipid challenge.Methods and resultsEighty‐six adult males and females of comparable age, anthropometric, and biochemical profiles completed an oral lipid tolerance test (OLTT). PBMC transcriptome was profiled following OLTT. Weighted gene coexpression networks were constructed separately for males and females. Functional ontology analysis of network modules was performed and hub genes identified. Two modules of interest were identified in females–an "inflammatory" module and an "energy metabolism" module. NLRP3, which plays a central role in inflammation and STARD3 that is involved in cholesterol metabolism, were identified as hub genes for the respective modules.ConclusionThe OLTT induced some gender‐specific correlations of gene coexpression network modules. In females, biological processes relating to energy metabolism and inflammation pathways were evident. This suggests a gender specific link between inflammation and energy metabolism in response to lipids. In contrast, G‐protein coupled receptor protein signaling pathway was common to both genders.
From chaperonins to Rubisco assembly and metabolic repair
Authors: Hayer‐Hartl Manajit 
Published: September 2017

AbstractRibulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) mediates the fixation of atmospheric CO2 in photosynthesis by catalyzing the carboxylation of the 5‐carbon sugar ribulose‐1,5‐bisphosphate (RuBP). Despite its pivotal role, Rubisco is an inefficient enzyme and thus has been a key target for bioengineering. However, efforts to increase crop yields by Rubisco engineering remain unsuccessful, due in part to the complex machinery of molecular chaperones required for Rubisco biogenesis and metabolic repair. While the large subunit of Rubisco generally requires the chaperonin system for folding, the evolution of the hexadecameric Rubisco from its dimeric precursor resulted in the dependence on an array of additional factors required for assembly. Moreover, Rubisco function can be inhibited by a range of sugar‐phosphate ligands. Metabolic repair of Rubisco depends on remodeling by the ATP‐dependent Rubisco activase and hydrolysis of inhibitors by specific phosphatases. This review highlights our work toward understanding the structure and mechanism of these auxiliary machineries.
Bacterial cupredoxin azurin hijacks cellular signaling networks: Protein–protein interactions and cancer therapy
Authors: Gao Meng , Zhou Jingjing , Su Zhengding , Huang Yongqi 
Published: September 2017

AbstractAzurin secreted by Pseudomonas aeruginosa is an anticancer bacteriocin, which preferentially enters human cancer cells and induces apoptosis or growth inhibition. It turns out that azurin is a multi‐target anticancer agent interfering in the p53 signaling pathway and the non‐receptor tyrosine kinases signaling pathway. This suggests that azurin exerts its anticancer activity by interacting with multiple targets and interfering in multiple steps in disease progression. Therefore, azurin could overcome resistance to therapy. Besides azurin, putative bacteriocins that possess functional properties similar to those of azurin have been identified in more bacteria species. A systematic investigation on the anticancer mechanisms of azurin and the azurin‐like bacteriocins will provide more and better options in cancer therapy. In this review, we summarize how azurin and the derived peptides hijack key cellular regulators or cell surface receptors to remodel the cellular signaling networks. In particular, we highlight the necessity of determining the structure of azurin/p53 complex and investigating the influence of post‐translational modifications on interactions between azurin and p53. Therapeutic applications of azurin and derived peptides are also discussed.
Monitoring type‐2 censored reliability data in multistage processes
Authors: Asadzadeh Shervisn , Kiadaliry Fatemeh 
Published: September 2017

AbstractIn this paper, we propose control charts to monitor the Weibull scale parameter of type‐2 censored reliability data in multistage processes. A cumulative sum control chart and 2 exponentially weighted moving average control charts based on conditional expected values are devised to detect decreases in the mean level of reliability‐related quality characteristic. The proposed control schemes are based on standard smallest extreme value distributions derived from Weibull processes to effectively account for the cascade property, which is the main characteristic of multistage processes. Subsequently, simulation study is conducted to evaluate the performance of the control charts using average run length criterion. Extra quadratic loss, performance comparison index, and relative average run length are also used to compare the detect ability of our proposed monitoring procedures. Moreover, sensitivity analysis is done to study the impact of failure number in the sample size and to investigate the robustness of the proposed monitoring procedures against the shift in the previous stage. Finally, a real case study in a glass bottle–making company is investigated to illustrate the performance of the competing control charts. The results reveal the superiority of the cumulative sum control chart.
Reliability evaluation for wireless sensor networks with chain structures using the universal generating function
Authors: Liu Qiang , Yin Xiao , Yang Xiao , Ma Yanbo 
Published: September 2017

AbstractIn different protocols, wireless sensor networks (WSNs) form different topologies. The reliability evaluation of a WSN whose topology is a chain is studied in this paper. A bidirectional data transmission tree (BDTT) model is established to describe the process of data fusion and transmission on a WSN with a chain structure. From the characteristics of the measurement errors, the number of fusions, and measurement credibility, 3 definitions for the reliability of a BDTT are presented. Based on the universal generating function technique, an algorithm is suggested for evaluating the reliability of a BDTT. In the algorithm, a BDTT model is divided into 2 branches, and 2 new composition operators are defined according to data fusion and transmission. We use the first operator to obtain the u‐function of each branch and the second one to combine the u‐functions of 2 branches and obtain the u‐function of the BDTT. The process of calculating the reliability of a BDTT is illustrated with an example, and the effects of measurement errors, the number of fusions, and measurement credibility on the system reliability are analyzed.
Fibonacci sequence for modelling stop bands in random microstructure
Authors: Gitman Inna M. , Song Yilang 
Published: September 2017

AbstractIn this paper the mathematical concept of the Fibonacci sequence has been introduced as an accurate and reliable tool to model randomness in a heterogeneous material. It is also argued, that this randomness plays an important role and can control the response of a heterogeneous material, subjected to dynamic loading, here an elastic wave propagating through the material. A particular dynamic phenomenon, the presence of band gaps, has been analysed. It has been shown that randomness, modelled using the Fibonacci sequence, introduced into the material's structure, increases the range of stop band frequencies.
Implications of Warm Rain in Shallow Cumulus and Congestus Clouds for Large-Scale Circulations
Authors: Nuijens Louise , Emanuel Kerry , Masunaga Hirohiko , L’Ecuyer Tristan 
Published: September 2017

AbstractSpace-borne observations reveal that 20–40% of marine convective clouds below the freezing level produce rain. In this paper we speculate what the prevalence of warm rain might imply for convection and large-scale circulations over tropical oceans. We present results using a two-column radiative–convective model of hydrostatic, nonlinear flow on a non-rotating sphere, with parameterized convection and radiation, and review ongoing efforts in high-resolution modeling and observations of warm rain. The model experiments investigate the response of convection and circulation to sea surface temperature (SST) gradients between the columns and to changes in a parameter that controls the conversion of cloud condensate to rain. Convection over the cold ocean collapses to a shallow mode with tops near 850 hPa, but a congestus mode with tops near 600 hPa can develop at small SST differences when warm rain formation is more efficient. Here, interactive radiation and the response of the circulation are crucial: along with congestus a deeper moist layer develops, which leads to less low-level radiative cooling, a smaller buoyancy gradient between the columns, and therefore a weaker circulation and less subsidence over the cold ocean. The congestus mode is accompanied with more surface precipitation in the subsiding column and less surface precipitation in the deep convecting column. For the shallow mode over colder oceans, circulations also weaken with more efficient warm rain formation, but only marginally. Here, more warm rain reduces convective tops and the boundary layer depth—similar to Large-Eddy Simulation (LES) studies—which reduces the integrated buoyancy gradient. Elucidating the impact of warm rain can benefit from large-domain high-resolution simulations and observations. Parameterizations of warm rain may be constrained through collocated cloud and rain profiling from ground, and concurrent changes in convection and rain in subsiding and convecting branches of circulations may be revealed from a collocation of space-borne sensors, including the Global Precipitation Measurement (GPM) and upcoming Aeolus missions.
The Experience of Genetic Counselors Working with Patients Facing the Decision of Pregnancy Termination after 24 Weeks Gestation
Authors: Graziani Rachel N. A. , Nemzer Laurie , Kerns Jennifer 
Published: September 2017

AbstractPrenatal genetic counselors are health care professionals who counsel women making reproductive decisions which include decisions such as terminating pregnancies due to fetal anomalies. Little is known about the experiences and practices of prenatal genetic counselors working with women who have the option of termination after 24 weeks gestation. In this national survey of 168 genetic counselors who have practiced prenatal genetic counseling, we asked about their general practice patterns, including indications for which termination is offered and types of abortion care services that are coordinated by genetic counselors. We report respondents’ self‐assessments of level of understanding of federal abortion law and abortion procedures. Seventy‐six percent of respondents have offered and counseled on termination after 24 weeks and 93% of respondents believe it is the responsibility of the counselor to discuss this option with patients. However, one‐third report that they have some or no understanding of the procedures and three‐quarters report that they have some or no understanding of federal abortion law. The results of this study provide insight into knowledge and experiences of genetic counselors working with these patients, allowing for improved genetic counselor training and continuing education to provide better guidance and develop more effective means of assisting patients.