bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting the miR-200c/LIN28B axis in acquired EGFR-TKI resistance non-small cell lung cancer cells harboring EMT features
Authors: Sato Hiroki , Shien Kazuhiko , Tomida Shuta , Okayasu Kazuhiro , Suzawa Ken , Hashida Shinsuke , Torigoe Hidejiro , Watanabe Mototsugu , Yamamoto Hiromasa , Soh Junichi , Asano Hiroaki , Tsukuda Kazunori , Miyoshi Shinichiro , Toyooka Shinichi 
Published: January 2017

AbstractMicroRNA (miR)-200 family members (miR-200s) are frequently silenced in advanced cancer and have been implicated in the process of epithelial-to-mesenchymal transition (EMT). We previously reported that miR-200s were silenced through promoter methylation in acquired EGFR-tyrosine kinase inhibitor (TKI) resistant non-small cell lung cancer (NSCLC) cells harboring EMT features. In this study, we examined the functional role of miR-200s in NSCLC cells and investigated a novel approach to overcoming acquired EGFR-TKI resistance. In the analysis of NSCLC cell lines, each of the miR-200s expression-silenced cell lines showed promoter methylation. Significant correlations between miR-200c silencing and several oncogenic pathway alterations, including EMT-changes and LIN28B overexpression, were observed in the database analysis. In addition, EGFR-wild type cell lines had lower miR-200s expression levels than EGFR-mutant cell lines. The introduction of miR-200c using pre-miR-200c caused LIN28B suppression in cells with acquired EGFR-TKI resistance that harbored EMT features. Interestingly, both the introduction of miR-200c and the knockdown of LIN28B produced an antitumor effect in acquired EGFR-TKI resistance cells, whereas these manipulations were not effective in parental cells. The miR-200c/LIN28B axis plays an important role in cells with acquired resistance to EGFR-TKI that harbor EMT features and might be a useful therapeutic target for overcoming resistance.
Atmospheric footprint of the recent warming slowdown
Authors: Liu Bo , Zhou Tianjun 
Published: January 2017

AbstractGrowing body of literature has developed to detect the role of ocean heat uptake and transport in the recent warming slowdown between 1998–2013; however, the atmospheric footprint of the slowdown in dynamical and physical processes remains unclear. Here, we divided recent decades into the recent hiatus period and the preceding warming period (1983–1998) to investigate the atmospheric footprint. We use a process-resolving analysis method to quantify the contributions of different processes to the total temperature changes. We show that the increasing rate of global mean tropospheric temperature was also reduced during the hiatus period. The decomposed trends due to physical processes, including surface albedo, water vapour, cloud, surface turbulent fluxes and atmospheric dynamics, reversed the patterns between the two periods. The changes in atmospheric heat transport are coupled with changes in the surface latent heat flux across the lower troposphere (below approximately 800 hPa) and with cloud-related processes in the upper troposphere (above approximately 600 hPa) and were underpinned by strengthening/weakening Hadley Circulation and Walker Circulation during the warming/hiatus period. This dynamical coupling experienced a phase transition between the two periods, reminding us of the importance of understanding the atmospheric footprint, which constitutes an essential part of internal climate variability.
Simulated Critical Differences for Speech Reception Thresholds
Authors: Pedersen Ellen Raben , Juhl Peter Møller 
Published: January 2017

Purpose
Critical differences state by how much 2 test results have to differ in order to be significantly different. Critical differences for discrimination scores have been available for several decades, but they do not exist for speech reception thresholds (SRTs). This study presents and discusses how critical differences for SRTs can be estimated by Monte Carlo simulations. As an application of this method, critical differences are proposed for a 5-word sentences test (a matrix test) using 2 widely implemented adaptive test procedures.

Method

For each procedure, simulations were performed for different parameters: the number of test sentences, the
j
factor, the distribution of the subjects' true SRTs, and the slope of the discrimination function. For 1 procedure and 1 parameter setting, simulation data are compared with results found by listening tests (experimental data).

Results
The critical differences were found to depend on the parameters tested, including interactive effects. The critical differences found by simulation agree with data found experimentally.

Conclusions
As the critical differences for SRTs rely on multiple parameters, they must be determined for each parameter setting individually. However, with knowledge of the test setup, rules of thumb can be derived.
Integrated optical reflective amplified modulator for indoor millimetre wave radio‐over‐fibre applications
Authors: Mekonnen K.A. , van Zantvoort J.H.C. , Tessema N.M. , Cao Z. , Tangdiongga E. , Koonen A.M.J. 
Published: January 2017

The use of a 35 GHz reflective electroabsorption modulator (REAM) monolithically integrated with a semiconductor optical amplifier (SOA) for indoor wideband analogue applications is demonstrated. Unlike REAMs, good performances over a wide range of input optical power and wavelength are observed, which mitigates the need for accurate control of the input signal, especially at remote sites where simplicity is strictly required. In addition, it provides amplification functions to compensate for the modulator insertion losses. The device exhibits an RF gain in excess of −22 dB and spurious‐free dynamic range of >103 dB Hz4/5. Implementing the rate‐adaptive discrete‐multitone modulation, 35 Gbit/s aggregate transmission speed is demonstrated for a short‐range wideband subcarrier multiplexed radio‐over‐fibre system.
Abundances of the light elements from UV (HST) and red (ESO) spectra in the very old star HD 84937
Authors: Spite M. , Peterson R. C. , Gallagher A. J. , Barbuy B. , Spite F. 
Published: January 2017

Aims. In order to provide a better basis for the study of mechanisms of nucleosynthesis of the light elements beyond hydrogen and helium in the oldest stars, the abundances of C, O, Mg, Si, P, S, K, and Ca have been derived from UV-HST and visible-ESO high resolution spectra in the old, very metal-poor star HD 84937, at a metallicity that is 1/200 that of the Sun’s. For this halo main-sequence turnoff star, the abundance determination of P and S are the first published determinations.
Methods. The LTE profiles of the lines were computed and fitted to the observed spectra. Wherever possible, we compared the abundances derived from the UV spectrum to abundances derived from the visible or near-infrared spectra, and also corrected the derived abundances for non-LTE effects. Three-dimensional (3D) CO5BOLD model atmospheres have been used to determine the abundances of C and O from molecular CH and OH bands.
Results. The abundances of these light elements relative to iron in HD 84937 are found to agree well with the abundances of these elements in classical metal-poor stars. Our HD 84937 carbon abundance determination points toward a solar (or mildly enhanced above solar) value of [C/Fe]. The modest overabundance of the α elements of even atomic number Z, typical of halo turnoff stars, is confirmed in this example. The odd-Z element P is found to be somewhat deficient in HD 84937, at [P/Fe] = −0.32, which is again consistent with the handful of existing determinations for turnoff stars of such low metallicity. We show that the abundance of oxygen, deduced from the OH band from 3D computations, is not compatible with the abundance deduced from the red oxygen triplet. This incompatibility is explained by the existence of a chromosphere heating the shallow layers of the atmosphere where the OH band, in 3D computations, is mainly formed.
Conclusions. The abundance ratios are compared to the predictions of models of galactic nucleosynthesis and evolution.
Approach to Total Knee Replacement: A Randomized Double Blind Study between Medial Parapatellar and Midvastus Approach in the Early Postoperative Period in Asian Population
Authors: Aslam Mohammed , Sabir Aamir , Abbas Sohail , Tiwari Anurag , Singh Pritish , Tiwari Vivek 
Published: January 2017

AbstractThe purpose of this randomized study was to compare clinical and surgical outcomes of total knee replacements (TKRs) in the early postoperative period using midvastus approach versus medial parapatellar approach in Asian population in a double blind manner. Forty-two knees each were operated using midvastus approach and the medial parapatellar approach. Clinical parameters that were evaluated included Knee Society score (KSS); knee pain using visual analogue scale (VAS) on day 1, 1 week, and 1 month; time required to straight leg raise (SLR); patellar tracking; mean extensor lag at 1 week and 1 month; and time of discharge from the hospital. Surgical parameters that were evaluated included tourniquet time, incidence of lateral retinacular release, estimated blood loss, and any complications during the surgery. KSS at 1 week and 1 month postoperatively were significantly higher in the midvastus group as compared with medial parapatellar group; though similar at 3 months, 6 months, and 1 year. The patients in midvastus group required fewer number of lateral retinacular releases; achieved SLR earlier; had less mean extensor lag at 1 week; had less mean VAS score at day 1, 1 week, and 1 month; and had shorter hospital stay. There was no significant difference in the mean tourniquet time and estimated blood loss. One patient had patellar maltracking in the medial parapatellar group as compared with none in midvastus group. Midvastus approach to TKR results in quicker functional recovery with early discharge and rehabilitation in the Asian population as compared with medial parapatellar approach.
Buckelchirurgie: Old Fashion oder Cutting Edge?
Authors: Feltgen N. , Hoerauf H. 
Published: January 2017

ZusammenfassungEindellende Operationen waren über Jahrzehnte die einzige Behandlungsmöglichkeit bei der Versorgung der Ablatio retinae. In den vergangenen Jahren werden sie aber immer mehr von der Vitrektomie verdrängt, obwohl auch die zahlreichen vergleichenden klinischen Studien keine Evidenz für diesen Trend erkennen lassen. In diesem Beitrag sollen die relevanten Daten vorgestellt und die Indikationen für eine eindellende Operation dargestellt werden.
RETINAL PIGMENT EPITHELIAL ATROPHY AFTER ANTI–VASCULAR ENDOTHELIAL GROWTH FACTOR INJECTIONS FOR RETINAL ANGIOMATOUS PROLIFERATION
Authors: Hata Masayuki , Yamashiro Kenji , Oishi Akio , Ooto Sotaro , Tamura Hiroshi , Miyata Manabu , Ueda-Arakawa Naoko , Kuroda Yoshimasa , Takahashi Ayako , Tsujikawa Akitaka , Yoshimura Nagahisa 
Published: January 2017

Purpose:
To investigate the incidence rate and risk factors for development of retinal pigment epithelial (RPE) atrophy during anti–vascular endothelial growth factor (anti-VEGF) treatment for retinal angiomatous proliferation.

Methods:
This study included 46 eyes with treatment-naive retinal angiomatous proliferation. All patients were treated with ranibizumab or aflibercept injections. Color fundus photography, spectral-domain optical coherence tomography, and fundus autofluorescence were evaluated for RPE atrophy diagnosis. Baseline characteristics and gene polymorphisms of ARMS2 A69S, and CFH I62V were analyzed for association with development and progression of RPE atrophy.

Results:
Among 21 eyes treated with ranibizumab without preexisting RPE atrophy at baseline, 5 eyes (23.8%) developed RPE atrophy at 12 months. Among 20 eyes treated with aflibercept without preexisting RPE atrophy at baseline, 10 eyes (50.0%) developed RPE atrophy at 12 months. Refractile drusen at baseline was associated with RPE atrophy development at 12 months (P = 0.014), and the progression rate of RPE atrophy area was negatively correlated with subfoveal choroidal thickness at baseline (R = −0.595, P = 0.019). Gene polymorphisms were not associated with RPE atrophy.

Conclusion:
Retinal pigment epithelial atrophy developed in 36.6% during 12 months after anti-VEGF treatment for retinal angiomatous proliferation. The presence of refractile drusen at baseline was identified as a novel significant risk factor for RPE atrophy development.
CHORIOCAPILLARIS SIGNAL VOIDS IN MATERNALLY INHERITED DIABETES AND DEAFNESS AND IN PSEUDOXANTHOMA ELASTICUM
Authors: Spaide Richard F. 
Published: January 2017

Purpose:
To evaluate the pattern of choriocapillaris signal voids in maternally inherited diabetes and deafness and in pseudoxanthoma elasticum in eyes before the development of any geographic atrophy.

Methods:
The choriocapillaris under the central macula was imaged with the Optovue RTVue XR Avanti using a 10 μm slab thickness. Automatic local thresholding of the resultant raw data extracted areas of absent flow signal, called signal voids, and these were counted and logarithmically binned. The signal void patterns were analyzed in four eyes of two patients with maternally inherited diabetes and deafness and four eyes of three patients with pseudoxanthoma elasticum. None of the patients had geographic atrophy. These data were compared with 55 eyes of 38 healthy control subjects and analyzed with generalized estimating equations.

Results:
The choriocapillaris images in maternally inherited diabetes and deafness and pseudoxanthoma elasticum show that the model of signal voids followed a power law distribution, but with a slope and offset much lower than the normal control group, adjusted for age (P < 0.001). The eyes in the disease group were much more likely to have signal voids greater than 40,000 μm2.

Conclusion:
Before the development of any overt geographic atrophy, patients with maternally inherited diabetes and deafness and pseudoxanthoma elasticum show pronounced abnormalities of choriocapillaris flow. Current clinical measures of retinal pigment epithelial health only look for areas of cell death, as in geographic atrophy. It is not possible to determine from current imaging if the choriocapillaris loss precedes potential loss of function of the retinal pigment epithelium, such as secretion of vascular endothelial growth factor.
SURGICAL MANAGEMENT OF RETINAL DETACHMENT IN OSTEOGENESIS IMPERFECTA: CASE REPORT AND REVIEW OF THE LITERATURE
Authors: Fleissig Efrat , Barak Adiel 
Published: January 2017

Purpose:
To describe a case of scleral buckling in a patient with severe (Type III) osteogenesis imperfecta.

Methods:
Single interventional case report of a 37-year-old woman, with clinically diagnosed osteogenesis imperfecta Type III, presenting with an inferior retinal detachment of the right eye. The patient was promptly treated with scleral buckling.

Results:
Long-term follow-up has shown a good clinical outcome, with no recurrence of the retinal detachment. Eighteen months postsurgery, an ischemic branch retinal vein obstruction has developed in the right eye, complicated by macular edema. The patient was treated successfully with bevacizumab injections, with restoration of visual acuity to 20/25.

Conclusion:
Scleral buckle may provide a good surgical option in selected patients with osteogenesis imperfecta, yielding excellent anatomical and functional results.
SURGICAL MANAGEMENT OF TRACTIONAL RETINOSCHISIS ASSOCIATED WITH VITREOUS HEMORRHAGE IN RETINOPATHY OF PREMATURITY
Authors: Todorich Bozho , Thanos Aristomenis , Yonekawa Yoshihiro , Korot Edward , Trese Michael T. , Drenser Kimberly A. , Capone Antonio 
Published: January 2017

Purpose:
The tractional retinoschisis is a poorly understood, rare, and likely underappreciated entity in retinopathy of prematurity. The purpose of this article is to describe clinical findings and surgical management of tractional retinoschisis in retinopathy of prematurity, masquerading as Stage 4 retinopathy of prematurity retinal detachment.

Methods:
A retrospective review of a single case with literature review and case discussion.

Results:
In this report, we describe a child with retinopathy of prematurity and tractional schisis, who initially presented with vitreous hemorrhage and was effectively managed by vitrectomy and inner wall retinectomy. At 5 months after vitrectomy, the child demonstrated complete collapse of the retinoschisis with intact posterior pole and brisk light perception.

Conclusion:
Vitrectomy with or without inner wall retinectomy is effective in the management of tractional retinoschisis.
CHOROIDAL BLOOD VESSELS IN RETINAL PIGMENT EPITHELIAL ATROPHY USING OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY
Authors: Maruko Ichiro , Koizumi Hideki , Sawaguchi Shota , Hasegawa Taiji , Arakawa Hisaya , Iida Tomohiro 
Published: January 2017

Purpose:
To observe choroidal blood vessels in cases sharply demarcated with retinal pigment epithelium (RPE) atrophy at the macular area using en-face optical coherence tomography angiography (OCTA) and standard en-face optical coherence tomography (OCT).

Methods:
The retrospective study included 12 eyes (8 patients; 5 men, 3 women; average age, 64.3 years) with RPE atrophy seen by ophthalmoscopy. The macular areas with normal and atrophic RPE sections were examined using en-face OCTA with an 840-nm light source (RTVue XR Avanti; Optovue Inc), which system can obtain standard en-face OCT images at identical areas. Images on en-face OCTA and standard en-face OCT were compared with each other when the segmentation range was being set as the full thickness of the choroid by the attached software.

Results:
Choroidal blood vessels were seen as black on standard en-face OCT images in all cases. However, the choroidal vessels on OCTA were depicted as white in the RPE atrophic area and black in the normal areas.

Conclusion:
Choroidal blood vessels in the normal sections of RPE were visualized in black on standard en-face OCT and OCTA with an 840-nm light source; however, those in the atrophic parts were observed as white, or with bloodstream, on OCTA images. Choroidal blood vessels cannot be evaluated accurately in commercially available OCTA systems because of blockage of light by the RPE.
Anxiety and the Quality of Life of Children Living With Parental Cancer
Authors: Hauken May Aasebø , Senneseth Mette , Dyregrov Atle , Dyregrov Kari 
Published: January 2017

Background:
Previous research on children living with parental cancer has mainly focused on the psychosocial challenges, but few studies have explored their health-related quality of life (HRQOL). This is important to promote well-being and discover areas of distress, as well as positive aspects of the children’s life.

Objective:
The aim of this study was to study how children’s HRQOL is influenced by anxiety and whether age and gender act as moderators for this relationship.

Methods:
This study used a survey with a cross-sectional design, including 35 children between 8 and 18 years old (mean, 13.3 years old) living with parental cancer. Questionnaires of HRQOL (Kinder Lebensqualität) and anxiety (Revised Child Manifest Anxiety Scale) were used.

Results:
The children reported higher anxiety and lower HRQOL than the controls. The children’s physiological (P = .03), emotional (P = .04), and school (P = .00) functions were significantly impaired, whereas they scored in line with the controls on self-esteem, family, friends, and overall HRQOL. A negative correlation (r = −0.707, P < .01) between anxiety and HRQOL was found. Neither age nor gender acted as a moderator between anxiety and HRQOL.

Conclusions:
A one-dimensional focus on anxiety may not capture these children’s multidimensional challenges. In contrast, a focus on HRQOL may give important knowledge of the children’s challenges, as well as areas where they function well.

Implications for Practice:
Healthcare professionals need to work collaboratively across disciplines and have a multidimensional focus in caring for patients with cancer who have children. They must provide both the parents and children with adequate information and tools to handle their family health situation to promote the children’s HRQOL.
Increasing the time resolution of single-molecule experiments with Bayesian inference
Authors: Kinz-Thompson Colin D. , Gonzalez Ruben L. 
Published: January 2017

AbstractMany time-resolved, single-molecule biophysics experiments seek to characterize the kinetics of biomolecular systems exhibiting dynamics that challenge the time resolution of the given technique. Here we present a general, computational approach to this problem that employs Bayesian inference to learn the underlying dynamics of such systems, even when they are much faster than the time resolution of the experimental technique being used. By accurately and precisely inferring rate constants, our Bayesian Inference for the Analysis of Sub-temporal-resolution Data (BIASD) approach effectively enables the experimenter to super-resolve the poorly resolved dynamics that are present in their data.
Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system
Authors: Li Jun , Zhang Chi , Huang Poyi , Kuru Erkin , Forster-Benson Eliot T. C. , Li Taibo , Church George M. 
Published: January 2017

ABSTRACTReconstituted cell-free protein synthesis systems such as the Protein synthesis Using Recombinant Elements (PURE) system give high-throughput and controlled access toin vitroprotein synthesis. Here we show that compared to the commercial S30 crude extract based RTS 100E. coliHY system, the PURE system has less mRNA degradation and produces ~4-fold more total protein. However the majority of these polypeptides are partially translated or inactive since the signal from firefly luciferase (Fluc) translated in PURE is only ~2/3rdof that measured using the S30 crude extract system. Both of the two systems suffer from low ribosome recycling efficiency when translating proteins from 90 kD to 220 kD. A systematic fed-batch analysis of PURE shows replenishment of 6 small molecule substrates individually or in combination prior to energy depletion increased Fluc protein yield by ~1.5 to ~2-fold, while accumulation of inorganic phosphate contributes to reaction termination. Additionally, while adding EF-P to PURE reduced total protein translated, it also increased the fraction of active product and reduced partial translated product probably by slowing down the translation process. Finally, ArfA, rather than YaeJ or PrfH, helped reduce ribosome stalling when translating Fluc and improved system productivity in a template-dependent fashion.
Gene expression profiling reveals U1 snRNA regulates cancer gene expression
Authors: Cheng Zhi , Sun Yu , Niu Xiaoran , Shang YingChun , Wu Zhenfeng , Shi Jinsong , Gao Shan , Zhang Tao 
Published: January 2017

AbstractU1 small nuclear RNA (U1 snRNA), as one of the most abundant noncoding RNA in eukaryotic cells plays an important role in splicing of pre-mRNAs. Compared to other studies which have focused on the primary function of U1 snRNA and the neurodegenerative diseases caused by the abnormalities of U1 snRNA, this study is to investigate how the U1 snRNA over-expression affects the expression of genes on a genome-wide scale. In this study, we built a model of U1 snRNA over-expression in a rat cell line. By comparing the gene expression profiles of U1 snRNA over-expressed cells with those of their controls using the microarray experiments, 916 genes or loci were identified significantly differentially expressed. These 595 up-regulated genes and 321 down-regulated genes were further analyzed using the annotations from the GO terms and the KEGG database. As a result, three of 12 enriched pathways are well-known cancer pathways, while nine of them were associated to cancers in previous studies. The further analysis of 73 genes involved in 12 pathways suggests that U1 snRNA regulates cancer gene expression. The microarray data with ID GSE84304 is available in the NCBI GEO database.