bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Silk Fibroin Bio-Transient Solution Processable Memristor
Authors: Yong Jason , Hassan Basem , Liang You , Ganesan Kumaravelu , Rajasekharan Ranjith , Evans Robin , Egan Gary , Kavehei Omid , Li Jingliang , Chana Gursharan , Nasr Babak , Skafidas Efstratios 
Published: November 2017

AbstractToday’s electronic devices are fabricated using highly toxic materials and processes which limits their applications in environmental sensing applications and mandates complex encapsulation methods in biological and medical applications. This paper proposes a fully resorbable high density bio-compatible and environmentally friendly solution processable memristive crossbar arrays using silk fibroin protein which demonstrated bipolar resistive switching ratio of 104 and possesses programmable device lifetime characteristics before the device gracefully bio-degrades, minimizing impact to environment or to the implanted host. Lactate dehydrogenase assays revealed no cytotoxicity on direct exposure to the fabricated device and support their environmentally friendly and biocompatible claims. Moreover, the correlation between the oxidation state of the cations and their tendency in forming conductive filaments with respect to different active electrode materials has been investigated. The experimental results and the numerical model based on electro-thermal effect shows a tight correspondence in predicting the memristive switching process with various combinations of electrodes which provides insight into the morphological changes of conductive filaments in the silk fibroin films.
Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy
Authors: Sellés Julien , Penrad-Mobayed May , Guillaume Cyndélia , Fuger Alica , Auvray Loïc , Faklaris Orestis , Montel Fabien 
Published: November 2017

AbstractNuclear Pore Complex (NPC) is of paramount importance for cellular processes since it is the unique gateway for molecular exchange through the nucleus. Unraveling the modifications of the NPC structure in response to physiological cues, also called nuclear pore plasticity, is key to the understanding of the selectivity of this molecular machinery. As a step towards this goal, we use the optical super-resolution microscopy method called direct Stochastic Optical Reconstruction Microscopy (dSTORM), to analyze oocyte development impact on the internal structure and large-scale organization of the NPC. Staining of the FG-Nups proteins and the gp210 proteins allowed us to pinpoint a decrease of the global diameter by measuring the mean diameter of the central channel and the luminal ring of the NPC via autocorrelation image processing. Moreover, by using an angular and radial density function we show that development of the Xenopus laevis oocyte is correlated with a progressive decrease of the density of NPC and an ordering on a square lattice.
Protective effect of HTK solution on postoperative pulmonary function in infants with CHD and PAH
Authors: Li Jindong , Wu Yanhong , Tian Xudong , Wang Jiantang , Dong Mingfeng , Wang Anbiao , Ma Shengjun 
Published: November 2017

Objective: In the present study, we aimed to investigate the effect of pulmonary arterial perfusion (PAP) with Histidine–tryptophan–ketoglutarate (HTK) on lung protection in infants with congenital heart disease (CHD) and pulmonary arterial hypertension (PAH) after cardiopulmonary bypass (CPB).
Methods: Fifty infant patients with CHD and PAH at our hospital from January, 2016 to February, 2017 were randomly divided into control group and HTK group. The levels of interleukin-6 (IL-6), malondialdehyde (MDA), and endothelin-1 (ET-1) in serum were detected using ELISA Kit. Oxygen index (OI) and respiratory index (RI) were calculated at each time point. The time of postoperative mechanical ventilation and ICU stay was counted, and the right lower lung tissues in patients were taken for pathological examination.
Results: Compared with preanesthesia, the levels of IL-6, MDA, and ET-1 in the two groups were significantly increased after CPB, and their levels in HTK group were significantly lower than that in control group. Moreover, OI in control group decreased markedly and RI in control group increased significantly after CPB. Compared with control group, the postoperative mechanical ventilation time, postoperative ICU stay, and total hospital stay in HTK group were markedly short. In addition, inflammatory cells infiltration decreased and pulmonary interstitial showed mild edema in HTK group.
Conclusion: PAP with HTK could effectively reduce CPB-induced lung injury and improve lung function.
The immunopeptidomic landscape of ovarian carcinomas
Authors: Schuster Heiko , Peper Janet K. , Bösmüller Hans-Christian , Röhle Kevin , Backert Linus , Bilich Tatjana , Ney Britta , Löffler Markus W. , Kowalewski Daniel J. , Trautwein Nico , Rabsteyn Armin , Engler Tobias , Braun Sabine , Haen Sebastian P. , Walz Juliane S. , Schmid-Horch Barbara , Brucker Sara Y. , Wallwiener Diethelm , Kohlbacher Oliver , Fend Falko , Rammensee Hans-Georg , Stevanović Stefan , Staebler Annette , Wagner Philipp 
Published: November 2017

SignificanceDespite the revolution in cancer therapy initiated by checkpoint inhibitors, durable clinical responses remain sporadic in many types of cancer, including ovarian cancer. Understanding which antigens are essentially presented by tumor cells and further able to be recognized by T cells provides a major step toward novel effective targeted immunotherapies. In this study, we comprehensively analyzed the immunopeptidomic landscape of ovarian carcinoma and compared it to variety of benign sources to identify antigens exclusively presented on tumor cells. With personalized therapies moving into the focus of clinical cancer therapy, we further present insights on how gene-expression analysis and immunohistochemistry can support antigen selection for individualized immunotherapy.
Effect of gaseous carbon dioxide on grain refinement in Mg-8Al alloy
Authors: Liu Yan , You Guoqiang , Gao Fan , Long Siyuan , Liu Qing , Shao Jun , Ao Sihai , Li Xiangguang 
Published: November 2017

A Mg-Al alloy was treated with gaseous carbon dioxide (CO2) and a refining process, and the grain refinement at each stage was investigated. The results indicated that CO2 and the subsequent refining process affect the grain refinement in the Mg-Al alloy. Moreover, the average grain size decreased from 254 to 89 µm, and the microhardness increased from 59.7 to 66.0 HV upon the addition of 9 litres of CO2. Increasing the time of the subsequent refining process to 30 min further decreased the grain size and increased the microhardness. Based on scanning electron microscopy, energy-dispersive spectroscopy and X-ray diffraction analyses, the observed Al4C3 particles, which are attributed to the inoculation of CO2, are responsible for the grain refinement. This paper is part of a thematic issue on Light Alloys.
Application of Bacillus sp. TAT105 to reduce ammonia emissions during pilot-scale composting of swine manure
Authors: Kuroda Kazutaka , Tanaka Akihiro , Furuhashi Kenich , Nakasaki Kiyohiko 
Published: November 2017

Abstract
Thermophilic ammonium-tolerant bacterium Bacillus sp. TAT105 grows and reduces ammonia (NH3) emissions by assimilating ammonium nitrogen during composting of swine feces. To evaluate the efficacy of a biological additive containing TAT105 at reducing NH3 emissions, composting tests of swine manure on a pilot scale (1.8 m3) were conducted. In the TAT105-added treatment, NH3 emissions and nitrogen loss were lower than those in the control treatment without TAT105. No significant difference was detected in losses in the weight and volatile solids between the treatments. Concentration of thermophilic ammonium-tolerant bacteria in the compost increased in both treatments at the initial stage of composting. In the TAT105-added treatment, bacterial concentration reached ~109 colony-forming units per gram of dry matter, several-fold higher than that in the control and stayed at the same level until the end. These results suggest that TAT105 grows during composting and reduces NH3 emissions in TAT105-added treatment.
Preparation and biological activities of anti-HER2 monoclonal antibodies with fully core-fucosylated homogeneous bi-antennary complex-type glycans
Authors: Tsukimura Wataru , Kurogochi Masaki , Mori Masako , Osumi Kenji , Matsuda Akio , Takegawa Kaoru , Furukawa Kiyoshi , Shirai Takashi 
Published: November 2017

Abstract
Recently, the absence of a core-fucose residue in the N-glycan has been implicated to be important for enhancing antibody-dependent cellular cytotoxicity (ADCC) activity of immunoglobulin G monoclonal antibodies (mAbs). Here, we first prepared anti-HER2 mAbs having two core-fucosylated N-glycan chains with the single G2F, G1aF, G1bF, or G0F structure, together with those having two N-glycan chains with a single non-core-fucosylated corresponding structure for comparison, and determined their biological activities. Dissociation constants of mAbs with core-fucosylated N-glycans bound to recombinant Fcγ-receptor type IIIa variant were 10 times higher than those with the non-core-fucosylated N-glycans, regardless of core glycan structures. mAbs with the core-fucosylated N-glycans had markedly reduced ADCC activities, while those with the non-core-fucosylated N-glycans had high activities. These results indicate that the presence of a core-fucose residue in the N-glycan suppresses the binding to the Fc-receptor and the induction of ADCC of anti-HER2 mAbs.
Insulin-Like Growth Factor-1 and Receptor Contribute Genetic Susceptibility to Hypertension in a Han Chinese Population
Authors: Yao Yingshui , Zhu Lijun , Fang Zhengmei , Yang Song , Chen Yanchun , Jin Yuelong , Zhao Xianghai , Zhao Hailong , Zhuang Qian , Chen Xiaotian , Shen Chong 
Published: November 2017

Abstract

BACKGROUND
Insulin-like growth factor 1 (IGF-1) and IGF-1 receptor (IGF-1R) have been suggested to mediate the pathophysiological response to elevated blood pressure. This study aims to evaluate the association of IGF-1 and IGF-1R with hypertension.

METHODS
Overall, 2,012 hypertensive cases and 2,210 controls were included in a case–control study, and 10 tagging single nucleotide polymorphisms (tagSNPs) were selected. The association of these SNPs with hypertension was further evaluated in a follow-up analysis and in an adolescent population.

RESULTS
A case–control study indicated that rs1815009 and rs2654981 in IGF-1R were significantly associated with hypertension, with odds ratios of 0.89 (P = 0.009) and 1.19 (P = 0.034), respectively, after adjusting for covariates. Stratification analyses revealed significant associations with hypertension (P &lt; 0.05) for rs35767 in normal weight and obese populations; for rs2229765 in individuals &lt;55 years of age and in overweight and nondrinking populations; and for rs2002880 in overweight and drinking populations. In a follow-up study, rs13379905 in IGF-1R was associated with hypertension incidence (hazard ratio, HR = 1.24, P = 0.042). This association was more significant in individuals with a hypertensive family history (HR = 2.10, P = 0.001). The association of rs13379905 with prehypertension and hypertension was further replicated in adolescent males (P = 0.005). Significant associations with hypertension incidence (P &lt; 0.05) were observed for rs6219 in individuals &lt;55 years of age and among those with obesity and a hypertensive family history as well as rs2002880 in obese individuals.

CONCLUSIONS
Our findings suggest that IGF-1R may contribute to the genetic susceptibility to hypertension, with BMI, age, and family history of hypertension all potentially modulating the genetic effects of IGF-1 on hypertension.
The heart regulates the endocrine response to heart failure: cardiac contribution to circulating neprilysin
Authors: Arrigo Mattia , Vodovar Nicolas , Nougué Hélène , Sadoune Malha , Pemberton Chris J , Ballan Pamela , Ludes Pierre-Olivier , Gendron Nicolas , Carpentier Alain , Cholley Bernard , Bizouarn Philippe , Cohen-Solal Alain , Singh Jagmeet P , Szymonifka Jackie , Latremouille Christian , Samuel Jane-Lise , Launay Jean-Marie , Pottecher Julien , Richards A Mark , Truong Quynh A , Smadja David M , Mebazaa Alexandre 
Published: November 2017

Abstract

Aims
Heart failure (HF) is accompanied by major neuroendocrine changes including the activation of the natriuretic peptide (NP) pathway. Using the unique model of patients undergoing implantation of the CARMAT total artificial heart and investigating regional differences in soluble neprilysin (sNEP) in patients with reduced or preserved systolic function, we studied the regulation of the NP pathway in HF.

Methods and results
Venous blood samples from two patients undergoing replacement of the failing ventricles with a total artificial heart were collected before implantation and weekly thereafter until post-operative week 6. The ventricular removal was associated with an immediate drop in circulating NPs, a nearly total disappearance of circulating glycosylated proBNP and furin activity and a marked decrease in sNEP. From post-operative week 1 onwards, NP concentrations remained overall unchanged. In contrast, partial recoveries in glycosylated proBNP, furin activity, and sNEP were observed. Furthermore, while in patients with preserved systolic function (n = 6), sNEP concentrations in the coronary sinus and systemic vessels were similar (all P > 0.05), in patients with reduced left-ventricular systolic function, sNEP concentration, and activity were ∼three-fold higher in coronary sinus compared to systemic vessels (n = 21, all P < 0.0001), while the trans-pulmonary gradient was neutral (n = 5, P = 1.0).

Conclusion
The heart plays a pivotal role as a regulator of the endocrine response in systolic dysfunction, not only by directly releasing NPs but also by contributing to circulating sNEP, which in turn determines the bioavailability of other numerous vasoactive peptides.
Outcomes of Operative Treatment for Adult Cervical Deformity: A Prospective Multicenter Assessment With 1-Year Follow-up
Authors: Ailon Tamir , Smith Justin S , Shaffrey Christopher I , Kim Han Jo , Mundis Gregory , Gupta Munish , Klineberg Eric , Schwab Frank , Lafage Virginie , Lafage Renaud , Passias Peter , Protopsaltis Themistocles , Neuman Brian , Daniels Alan , Scheer Justin K , Soroceanu Alex , Hart Robert , Hostin Rick , Burton Douglas , Deviren Vedat , Albert Todd J , Riew K Daniel , Bess Shay , Ames Christopher P ,   International Spine Study Group
Published: November 2017

Abstract

BACKGROUND
Despite the potential for profound impact of adult cervical deformity (ACD) on function and health-related quality of life (HRQOL), there are few high-quality studies that assess outcomes of surgical treatment for these patients.

OBJECTIVE
To determine the impact of surgical treatment for ACD on HRQOL.

METHODS
We conducted a prospective cohort study of surgically treated ACD patients eligible for 1-yr follow-up. Baseline deformity characteristics, surgical parameters, and 1-yr HRQOL outcomes were assessed.

RESULTS
Of 77 ACD patients, 55 (71%) had 1-yr follow-up (64% women, mean age of 62 yr, mean Charlson Comorbidity Index of 0.6, previous cervical surgery in 47%). Diagnoses included cervical sagittal imbalance (56%), cervical kyphosis (55%), proximal junctional kyphosis (7%) and coronal deformity (9%). Posterior fusion was performed in 85% (mean levels = 10), and anterior fusion was performed in 53% (mean levels = 5). Three-column osteotomy was performed in 24% of patients. One year following surgery, ACD patients had significant improvement in Neck Disability Index (50.5 to 38.0, P < .001), neck pain numeric rating scale score (6.9 to 4.3, P < .001), EuroQol 5 dimension (EQ-5D) index (0.51 to 0.66, P < .001), and EQ-5D subscores: mobility (1.9 to 1.7, P = .019), usual activities (2.2 to 1.9, P = .007), pain/discomfort (2.4 to 2.1, P < .001), anxiety/depression (1.8 to 1.5, P = .014).

CONCLUSION
Based on a prospective multicenter series of ACD patients, surgical treatment provided significant improvement in multiple measures of pain and function, including Neck Disability Index, neck pain numeric rating scale score, and EQ-5D. Further follow-up will be necessary to assess the long-term durability of these improved outcomes.
Human Brucellosis in Rural Uganda: Clinical Manifestations, Diagnosis, and Comorbidities at Kabale Regional Referral Hospital, Kabale, Uganda
Authors: Dieckhaus Kevin D , Kyebambe Peterson S 
Published: November 2017

Abstract

Background
Brucellosis is a zoonotic infection transmitted to humans through direct contact with infected animals, their products, or excreta such as urine or dung. Brucellosis is associated with significant morbidity in Southwestern Uganda, where cattle and goat rearing are a major economic industry. As in many settings in sub-Saharan Africa, diagnosis and management of brucellosis remain a challenge due to the presence of comorbidities and limitations in resources for diagnostic testing and therapy.

Methods
A chart review was conducted to characterize the clinical manifestations, diagnosis, comorbidities, and management of 101 patients treated for brucellosis at the Kabale Regional Referral Hospital from September 2002 to May 2010.

Results
Patients presented with substantial comorbidities. The most common manifestation of illness was osteoarticular, but disease manifestations were quite varied. A high rate of focal illness in this cohort (77%) was observed.

Conclusions
Clinicians in this setting should be cognizant of the varied presentations, comorbidities, and treatment options for this disease.
“The Questions Shape the Answers”: Assessing the Quality of Published Survey Instruments in Health Professions Education Research
Authors: Artino Anthony R. , Phillips Andrew W. , Utrankar Amol , Ta Andrew Q. , Durning Steven J. 
Published: November 2017

Purpose
Surveys are widely used in health professions education (HPE) research, yet little is known about the quality of the instruments employed. Poorly designed survey tools containing unclear or poorly formatted items can be difficult for respondents to interpret and answer, yielding low-quality data. This study assessed the quality of published survey instruments in HPE.

Method
In 2017, the authors performed an analysis of HPE research articles published in three high-impact journals in 2013. They included articles that employed at least one self-administered survey. They designed a coding rubric addressing five violations of established best practices for survey item design and used it to collect descriptive data on the validity and reliability evidence reported and to assess the quality of available survey items.

Results
Thirty-six articles met inclusion criteria and included the instrument for coding, with one article using 2 surveys, yielding 37 unique surveys. Authors reported validity and reliability evidence for 13 (35.1%) and 8 (21.6%) surveys, respectively. Results of the item-quality assessment revealed that a substantial proportion of published survey instruments violated established best practices in the design and visual layout of Likert-type rating items. Overall, 35 (94.6%) of the 37 survey instruments analyzed contained at least one violation of best practices.

Conclusions
The majority of articles failed to report validity and reliability evidence, and a substantial proportion of the survey instruments violated established best practices in survey design. The authors suggest areas of future inquiry and provide several improvement recommendations for HPE researchers, reviewers, and journal editors.
What Every Graduating Resident Needs to Know About Quality Improvement and Patient Safety: A Content Analysis of 26 Sets of ACGME Milestones
Authors: Lane-Fall Meghan B. , Davis Joshua J. , Clapp Justin T. , Myers Jennifer S. , Riesenberg Lee Ann 
Published: November 2017

Purpose
Quality improvement (QI) and patient safety (PS) are broadly relevant to the practice of medicine, but specialty-specific milestones demonstrate variable expectations for trainee competency in QI/PS. The purpose of this study was to develop a unifying portrait of QI/PS expectations for graduating residents irrespective of specialty.

Method
Milestones from 26 residency programs representing the 24 member boards of the American Board of Medical Specialties were downloaded from the Accreditation Council for Graduate Medical Education (ACGME) Web site in 2015. A codebook was generated by in-depth reading of all milestone sets by two authors. Using a content analytic approach, milestones were then coded by a single author, with a 25% sample double coded by another author. Descriptive statistics were used to characterize frequency counts.

Results
Of 612 total milestones, 249 (40.7%) made mention of QI/PS. A median 10 milestones per specialty (interquartile range, 5.25–11.75) mentioned QI/PS. There were 446 individual references to QI, 423 references to PS, and another 1,065 references to QI/PS-related concepts, including patient-centered care, cost-effective practice, documentation, equity, handoffs and care transitions, and teamwork. QI/PS references reflected expectations about both individual-level practice (531/869; 61.1%) and practice within a health care system (338/869; 38.9%). QI and PS references were linked to all six ACGME core competencies.

Conclusions
Although there is variability in the emphasis placed on QI/PS across specialties, overall, QI/PS is reflected in more than 40% of residency milestones. Graduating residents in all specialties are expected to demonstrate competence in QI, PS, and multiple related concepts.
Diagnostic Accuracy of Zero-Echo Time MRI for the Evaluation of Cervical Neural Foraminal Stenosis
Authors: Argentieri Erin C. , Koff Matthew F. , Breighner Ryan E. , Endo Yoshimi , Shah Parina H. , Sneag Darryl B. 
Published: November 2017

Study Design.
A cohort study.

Objective.
The aim of this study was to evaluate the clinical utility of Zero-Echo-Time (ZTE) magnetic resonance imaging (MRI) for the assessment of cervical neural foraminal stenosis (CNFS) through the comparison of inter-modality [computed tomography (CT) and ZTE-MRI] CNFS grade severity agreements.

Summary of Background Data.
Conventional MRI limited in its ability to provide direct visualization of cortical bone. The highly organized tissue structure of cortical bone results in very short T2 values that preclude acquisition of sufficient signal intensity and positive image contrast. ZTE imaging permits visualization of tissues with very short transverse relaxation times, and is capable of displaying images with CT-like contrast.

Methods.
Thirty-four subjects were recruited from a clinical cohort of patients undergoing standard of care MRI and CT imaging for evaluation of CNFS. Standard of care CT imaging studies were obtained on all subjects within 6 months of their ZTE-MRI acquisition (mean time interval: 25.3 ± 54.1 days; median: 0 days). ZTE-MRI and CT imaging studies were evaluated and severity of CNFS was graded on a scale from 0 to 5 (0 = none; 5 = severe). Weighted-kappa statistics were used to assess agreement between ZTE and CT grades of CNFS on both sides (right and left) of each motion segment. Ordinal logistic mixed-effects regression analyses evaluated the effects of inter-modality position differences (flexion-extension curvature) on inter-modality differences in CNFS grade.

Results.
Substantial agreement (κ = 0.72) was found between ZTE- and CT-based grades of CNFS. Significant inter-modality differences in cervical spine curvature were found for all motion segments, except C2–3 and C3–4 (P < 0.05). However, no significant relationship was found between inter-modality differences in curvature, and inter-modality differences in CNFS grade for any motion segment (P = 0.28).

Conclusion.
Results of the current study suggest that ZTE-MRI is well-suited for the evaluation of CNFS and may have the potential to obviate the need for concurrent CT scans in some cases.

Level of Evidence: 2