bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways
Authors: Cai Chuanchuan , Liu Si , Liu Yufeng , Huang Shaobin , Lu Shiya , Liu Fang , Luo Xiaohua , Zouboulis Christos C. , Shi Ge 
Published: October 2024

AbstractInsulin-like growth factor-1 (IGF-1) is considered as a pathogenic factor contributing to sebaceous gland dysfunction, which leads to acne vulgaris. Paeoniflorin (Pae), a bioactive monomer derived from total glycosides of paeony, has shown potential in treating various diseases. However, its anti-acne effects on human sebocytes are not well understood. In this study, we investigated the effects of Pae on acne development induced by IGF-1 in SZ95 sebocytes. Following IGF-1 stimulation, SZ95 sebocytes were exposed to Pae and then determined for proliferation, cell cycle, apoptosis, lipogenesis and pro-inflammatory cytokine secretion. We also analyzed the expression of proteins involved in the PI3K/Akt/FoxO1 and JAK2/STAT3 pathways. In vitro experiments demonstrated that Pae significantly inhibited colony formation, induced G1/S cell cycle arrest, promoted apoptosis, inhibited lipogenesis and cytokine synthesis in IGF-1-treated SZ95 sebocytes. Furthermore, Pae suppressed the phosphorylation of Akt, FoxO1, JAK2, and STAT3. Importantly, the sebo-suppressive and anti-inflammatory effects of Pae were enhanced by blocking PI3K and JAK2. In summary, our findings suggest that Pae has potent anti-proliferative and pro-apoptotic effects in SZ95 sebocytes. Additionally, Pae effectively protects against IGF-1-induced lipogenesis and inflammation by targeting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways.
Graphical Abstract
Perfusion Bioreactor Conditioning of Small-diameter Plant-based Vascular Grafts
Authors: Gorbenko Nicole , Vaccaro John C. , Fagan Ryan , Cerro Robert A. , Khorrami Jonah M. , Galindo Lucia , Merna Nick 
Published: October 2024

Abstract
Background:
Vascular grafts are mainly composed of synthetic materials, but are prone to thrombosis and intimal hyperplasia at small diameters. Decellularized plant scaffolds have emerged that provide promising alternatives for tissue engineering. We previously developed robust, endothelialized small-diameter vessels from decellularized leatherleaf viburnum. This is the first study to precondition and analyze plant-based vessels under physiological fluid flow and pressure waveforms. Using decellularized leatherleaf viburnum as tissue-engineered grafts for implantation can have profound impacts on healthcare due to their biocompatibility and cost-effective production.

Methods:
A novel perfusion bioreactor was designed, capable of accurately controlling fluid flow rate and pressure waveforms for preconditioning of small-diameter vascular grafts. A closed-loop system controlled pressure waveforms, mimicking physiological values of 50–120 mmHg at a frequency of 8.75 Hz for fluid flow reaching 5 mL/min. Plant-based vascular grafts were recellularized with endothelial and vascular smooth muscle cells and cultured for up to 3 weeks in this bioreactor. Cell density, scaffold structure and mechanics, thrombogenicity, and immunogenicity of grafts were evaluated.

Results:
Bioreactor treatment with fluid flow significantly increased luminal endothelial cell density, while pressure waveforms reduced thrombus formation and maintained viable vascular smooth muscle cells within inner layers of grafts compared to static controls. Suture retention of grafts met transplantation standards and white cell viability was suitable for vascular remodeling.

Conclusion:
Low thrombogenicity of endothelialized leatherleaf viburnum holds great potential for vascular repair. This study provides insight into benefits of conditioning plant-based materials with hemodynamic forces at higher frequencies that have not previously been investigated.
Development of Automotive and Marine Appliable Aluminium Composite by Utilizing Agro-Waste Material as Performance Enhancement Particles
Authors: Akande I. G. , Kazeem R. A. , Jen T.-C. , Daramola O. M. , Akinlabi E. T. 
Published: October 2024

Abstract
Aluminium-based materials are lightweight materials used for producing automotive and aircraft components. However, aluminium materials diminish in performance on exposure to degrading environments, which limits their areas of usage and applications. The degrading effect results in poor resistance to wear and corrosion, reduced properties and defective microstructure. In this work, 6063 aluminium alloy was reinforced with particles of agricultural waste (walnut shell) to produce six samples with five samples of reinforced and a control (unreinforced) sample. Each of the samples of the reinforced alloy was moulded into a 25 mm diameter by 130 mm height using the stir casting method using an industrial pit furnace. The samples were thereafter machined to a diameter of 20 mm and cut into a thickness of 10 mm for characterizations. The potentiodynamic polarization method was used to test for the samples’ corrosion resistance properties following the ASTM G102 standard in a 3.65% NaCl test medium. The hardness property was investigated using the Brinell hardness machine following the ASTM A-370 standard, while the microstructure and crystallographic phase studies were carried out using SEM/EDS and XRD profiles, respectively. The unreinforced 6063 Al alloy sample exhibited the highest corrosion rate (Cr) of 0.7321 mm/year and the lowest hardness of 104.94 kgf/mm2. The 10% wt. walnut shell particles (WSP) reinforced 6063 Al alloy sample exhibited the lowest corrosion rate (Cr) of 0.1336 mm/year and the highest hardness of 109.24 kgf/mm2. This indicated that the walnut shell particles enhanced the corrosion and indentation resistance of the alloy. In addition, the SEM images indicated that the agricultural waste (walnut shell particles) reinforced samples exhibited more refined microstructure, lower porosity and smoother morphology compared to the unreinforced (control) sample. Also, the XRD profile of samples revealed some high peak intensity crystallites such as Al(ZnS), Al2O3 and (FeMn)SiO2. These high peak intensity crystallites indicated that these reinforced samples possessed chemical and microstructural homogeneity, high stability and good surface texture.
Latent democratic potential: reflections on contemporary citizenship education in Singapore
Authors: Chia Yeow-Tong , Neoh Jia Ying 
Published: October 2024

AbstractDemocratic citizenship education aims to empower students for active participation in democratic deliberation and meaningful public action. However, defining 'democratic' is complex, and ideals vary. Singapore's education system has faced criticism for perceived shortcomings in preparing students for democratic engagement. Concerns include a lack of understanding of democracy, a tendency to accept hierarchy without critical assessment, and a superficial grasp of citizenship concepts. The social studies curriculum and overall school environment are often seen as limited in fostering democratic involvement. This is attributed in the literature to a lack of explicit commitment to democracy in Singapore's citizenship education. Instead, the system appears to lean towards uncritical adherence to national values and commitment to the nation. This paper challenges the focus on formal citizenship education by exploring the potential for democratic engagement within the broader Singapore curriculum. It argues that implicit democratic values emerge through initiatives like "Thinking School, Learning Nation" and the 21st-century competencies framework, fostering critical thinking, which is crucial for developing democratic dispositions. The analysis of alignment between competencies in Singapore education initiatives and those crucial for contemporary democratic participation offers a nuanced understanding of citizenship education beyond its traditional link to notions of liberal democracy. The paper contributes to debates about democracy's relevance in non-liberal democratic contexts, enriching our understanding of alternative pathways to civic engagement and possible futures for democratic citizenship.
The frequency and types of resident relocations in Dutch nursing homes: a nationwide cohort study of electronic health record data
Authors: Schreuder Miranda C. , Joling Karlijn J. , Groen Wim G. , Perry Marieke , Landeweer Elleke G. M. , Luijendijk Hendrika J. , Zuidema Sytse U. ,   the RELOCARE Consortium, Verbeek H. , Hamers J.P.H. , Schols J.M.G.A. , de Boer B. , Urlings J.H.J. , Brouwers M. , Achterberg W.P. , Caljouw M.A.A. , Landeweer E.G.M. , Luijendijk H.J. , Schreuder M.C. , Zuidema S.U. , Perry M , Koopmans R.T.C.M. , Luijkx K.G. , Stoop A. , Groen W.G. 
Published: October 2024

Abstract
Purpose
One third of Dutch nursing home residents relocated at least once during length of stay. Roughly 75 percent were individual relocations and the other 25 percent concerned group relocations. The average yearly number of individual relocations was about 3 times as high in the first 4 months after admission compared with later periods.

Methods
We performed an historical cohort study of pseudonymized registration data from a Dutch electronic health record. We selected data from nursing home residents aged 65 years and older who stayed on a ward for physical impairment or dementia and passed away between 2015 and 2019. Our study sample consisted of 26,060 long-stay nursing home residents from 67 nursing homes in the Netherlands. We examined the number of relocations, trends over time, individual versus group relocations and relocation destinations.

Results
We found that approximately one-third of long-stay nursing home residents relocated at least once with an average of 36 relocations per 100 residents per year. Roughly, 75 percent of relocations were individual relocations and 25 percent concerned group relocations. In the first 4 months after admission, the average number of individual relocations per 100 resident per year was about 3 times as often compared to later periods after admission. Most individual relocations were within the same type of care.

Conclusion
A considerable proportion of Dutch long-stay nursing home residents experienced one or more relocations. Relocations for individual reasons occurred mostly in the first months after admission. Further investigation is warranted to explore which factors lead to relocations.
Impact of levetiracetam and ethanol on memory, selected neurotransmitter levels, oxidative stress parameters, and essential elements in rats
Authors: Zwierzyńska Ewa , Klimczak Michał , Nasiadek Marzenna , Stragierowicz Joanna , Pietrzak Bogusława 
Published: October 2024

Abstract
Background
Ethanol disrupts brain activity and memory. There is evidence supporting the beneficial effect of levetiracetam on alcohol consumption. Therefore, the aim of the study was to examine whether levetiracetam has a protective activity against ethanol-induced memory impairment, alterations in selected neurotransmission activities, oxidative stress, and selected essential elements in rats.

Methods
The rats were given levetiracetam (300 mg/kg b.w. po) with ethanol for three weeks prior to behavioral tests. Spatial memory was tested using the Morris water maze, while recognition memory was evaluated using the Novel object recognition test. The GABA and glutamate concentration was determined in three rat brain regions (cerebellum, hippocampus, and cerebral cortex). Serum oxidative stress parameters and selected essential elements concentration (Cu, Mn, Zn, Fe, Mg) in the rat brain were analyzed.

Results
Levetiracetam administered with ethanol improved spatial memory, but did not affect abstinence-induced impairment. The drug also decreased ethanol-induced long-term recognition memory impairment. No alterations in glutamate levels were observed. GABA levels were elevated by levetiracetam in the cerebral cortex and by ethanol in the cerebellum. Ethanol increased catalase activity (CAT) and decreased superoxide dismutase activity (SOD) in the serum. Levetiracetam significantly increased the activity of SOD. Alcohol disrupted the levels of trace elements (Mn, Zn, Mg) in the rat brain. Additionally, levetiracetam alone increased Mg, Fe, and Cu concentrations while all animals receiving the drug also had significantly lower concentrations of Zn.

Conclusions
Levetiracetam had differential effects against ethanol-induced impairments. These findings could have important implications for future levetiracetam treatment in patients.
Diagnostic accuracy of point-of-care ultrasound (PoCUS) for the diagnosis of hip effusion in the pediatric emergency department
Authors: Katz-Dana Hadas , Stackievicz Rudica , Dana Elad , Friedman Nir , Lackner Gali , Rosenbloom Ehud , Shles Ayelet 
Published: October 2024

Abstract
Purpose
A new limp or refusal to weight-bear are common symptoms in children presenting to the pediatric emergency department (ED). This poses a diagnostic challenge, particularly among toddlers and nonverbal patients. Point-of-care ultrasound (PoCUS) used by pediatric emergency medicine physicians may detect hip effusion, which dramatically aids diagnostic workup and management. There is limited literature regarding the accuracy of hip PoCUS conducted by pediatric emergency medicine physicians. This study aims to assess the diagnostic performance of pediatric emergency medicine physician-performed PoCUS in identifying hip effusion.

Methods
This prospective study was conducted in a single-center pediatric ED. Children presenting with limb pain or new limp were evaluated by pediatric emergency medicine physicians who also performed hip PoCUS and categorized findings as either “effusion” or “no effusion” based on standard sonographic definitions. Patients also underwent radiology department ultrasound reviewed by a pediatric radiologist. Diagnostic test characteristics with corresponding 95% confidence intervals (CI) were calculated using radiology department ultrasound findings as the reference standard.

Results
A total of 95 patients were enrolled by 8 pediatric emergency medicine physicians. Excellent agreement was observed between PoCUS performed by pediatric emergency medicine physicians and radiology department ultrasound for the presence or absence of hip effusion (kappa = 0.81 [95% CI 0.70–0.93]). Hip effusion was identified by PoCUS in 44 out of 49 effusion-positive patients, with a sensitivity of 89.8% (95% CI 77.7–96.6%), specificity of 91.3% (95% CI 79.2%-97.5%), positive likelihood ratio of 10.33 (95% CI 4.03–26.47), and negative likelihood ratio of 0.11 (95% CI 0.05–0.26).

Conclusion
PoCUS performed by pediatric emergency medicine physicians has reasonably high sensitivity and specificity for diagnosing hip effusion among pediatric patients presenting to the pediatric ED with a limp or leg pain. This practice may potentially expedite both diagnosis and treatment within this patient population.
A retrospective review of LMNB1-related autosomal dominant leukodystrophy
Authors: Ortiz Judit M. Perez , Muthusamy Karthik , Tobin W. Oliver , Gavrilova Ralitza , Cousin Margot A. , Dhamija Radhika 
Published: October 2024

Abstract
Introduction
LMNB1-related autosomal dominant leukodystrophy (ADLD) is a slowly progressive neurodegenerative disorder caused by overexpression of LMNB1. We retrospectively reviewed charts of all ADLD patients seen at Mayo Clinic.

Methods
All available data from molecularly confirmed ADLD patients was reviewed.

Results
Of eight patients identified, three were male. Age at symptom onset ranged from 33 to 64 years. In males, the first symptom was erectile dysfunction (2/3) or neurogenic bladder (1/3) and, in females, weakness (3/5), bladder dysfunction (2/5), or depression (1/5). Diagnostic delay from symptom onset was a median of 6 (IQR 2.3–10) years. Other reported symptoms included cognitive difficulties (8/8), fatigue (7/8), sleep issues (4/8), mood disturbances (5/8), tremor (4/8), and migraine (4/8). Family history was positive in 6. All eight patients had LMNB1 duplication. Eighteen brain MRIs were reviewed from 7 patients. All showed symmetric confluent T2W deep cerebral and periventricular white matter hyperintensities with involvement of the posterior limb of the internal capsule, corpus callosum, corticospinal tract in brain stem, and superior and middle cerebellar peduncles. Seven spine MRIs from six patients showed moderate diffuse atrophy of the spinal cord.

Conclusion
Typical clinical symptoms and characteristic MRI changes should prompt genetic testing for ADLD.
Microneedle sensors for dermal interstitial fluid analysis
Authors: Kim Gwangmook , Ahn Hyunah , Chaj Ulloa Joshua , Gao Wei 
Published: October 2024

AbstractThe rapid advancement in personalized healthcare has driven the development of wearable biomedical devices for real-time biomarker monitoring and diagnosis. Traditional invasive blood-based diagnostics are painful and limited to sporadic health snapshots. To address these limitations, microneedle-based sensing platforms have emerged, utilizing interstitial fluid (ISF) as an alternative biofluid for continuous health monitoring in a minimally invasive and painless manner. This review aims to provide a comprehensive overview of microneedle sensor technology, covering microneedle design, fabrication methods, and sensing strategy. Additionally, it explores the integration of monitoring electronics for continuous on-body monitoring. Representative applications of microneedle sensing platforms for both monitoring and therapeutic purposes are introduced, highlighting their potential to revolutionize personalized healthcare. Finally, the review discusses the remaining challenges and future prospects of microneedle technology.
Graphical Abstract
Uncertainty quantification and sensitivity analysis of blade geometric deviation on compressor performance
Authors: Ji T.Y. , Chu W.L. , Guo Z.T. 
Published: October 2024

AbstractCarefully designing blade geometric parameters is necessary as they determine the aerodynamic performance of a rotor. However, manufacturing inaccuracies cause the blade geometric parameters to deviate randomly from the ideal design. Therefore, it is essential to quantify uncertainty and analyse the sensitivity of the blade geometric deviations on the compressor performance. This work considers a subsonic compressor rotor stage and examines samples with different geometry features using three-dimensional Reynolds-averaged Navier-Stokes simulations. A method to combine Halton sequence and non-intrusive polynomial chaos is adopted to perform the uncertainty quantitative (UQ) analysis. The Sobol’ index and Spearman correlation coefficient help analyse the sensitivity and correlation between the compressor performance and blade geometric deviations, respectively. The results show that the fluctuation amplitude of the compressor performance decreases for lower mass flow rates, and the sensitivity of the compressor performance to the blade geometrical parameters varies with the working conditions. The effects of various blade geometric deviations on the compressor performance are independent and linearly superimposed, and the combined effects of different geometric deviations on the compressor performance are small.
Invasion of superbugs: Cockroach-driven outbreak of multidrug-resistant Enterobacter in an ICU
Authors: Hanrahan Jennifer , Schouten Nicholas , Fyffe Steven H. , Jencson Annette , Stroever Stephanie 
Published: October 2024

Abstract

Objective:
To describe factors in an outbreak of multidrug-resistant Enterobacter cloacae (MRE) in an intensive care unit (ICU) over a 20-month period including the likely contribution of cockroaches to the outbreak.

Design:
This is a retrospective descriptive study.

Setting:
ICU in an urban hospital.

Patients:
All patients admitted to the ICU during the 20-month outbreak period were included in the study.

Interventions:
Infection prevention interventions included contact isolation, hand hygiene, dedicated patient equipment, environmental cultures, and pest control.

Results:
25 patients were identified as being colonized or infected with MRE. Relatedness of the outbreak strain and strains found in cockroaches was demonstrated by pulse field gel electrophoresis. Standard IP interventions did not have an impact on the outbreak until pest control was added. Once additional pest control measures were put in place, the outbreak ended.

Conclusions:
Insects have a potential role in transmission of pathogens in hospitals and their role should be considered when outbreaks are being investigated.
Relationship between defense styles and neurochemical variables of the hippocampus in patients with obsessive-compulsive disorder
Authors: Atmaca Murad , Gurok Mehmet Gurkan , Tabara Muhammed Fatih , Yildirim Hanefi 
Published: October 2024

AbstractThis study aims to assess the correlation between NAA (N-acetyl-l-aspartate), CHO (choline), and CRE (creatine) levels in the hippocampus regions of individuals suffering from obsessive-compulsive disorder (OCD) and defensive styles of the ego.The study group was composed of twenty patients with OCD and twenty healthy controls. NAA, CHO, and CRE values in the hippocampal region using proton magnetic resonance spectroscopy (1H-MRS) were measured. Participants’ defense styles were ascertained by administering the Defense Style Questionnaire-40.The patient group’s NAA levels were considerably lower than the control group’s on both sides of the hippocampus. The levels of CHO and CRE did not significantly differ between the two groups. The following statistically significant correlations were discovered: in the comparison group, there were negative correlations between the scores of mature defense styles and the right and left CHO levels, as well as between the immature defense mechanism scores and the right NAA levels in both the patient and control groups. In the patient group, there were also negative correlations between the left NAA values and the scores of mature defense styles.OCD patients have lower levels of NAA in the hippocampus. To validate and extend the current findings, more research involving a greater sample size is required.
The not-so-forbidden triad: Evaluating the assumptions of the Strength of Weak Ties
Authors: Neal Zachary P. 
Published: October 2024

AbstractThe Strength of Weak Ties is among the most influential social theories of the past 50 years. However, its prediction that weak ties are especially useful for obtaining novel information is sometimes not supported. To understand why, I investigate whether social networks typically satisfy the theory’s assumptions, and whether the theory’s prediction is robust to violations of its assumptions. First, examining a diverse corpus of 56 empirical social networks, I show that empirical social networks (nearly) satisfy some but not all of the theory’s assumptions. Second, using a simulation of information diffusion, I show that the predicted utility of weak ties is not robust to violations of these assumptions. When the assumptions of the theory are violated, as is common in social networks, access to novel information depends on bridging ties, regardless of their strength. Moreover, when they exist, strong bridges (i.e., bridges with high bandwidth) are more useful than weak bridges (i.e., bridges with low bandwidth). I conclude by recommending that research applying this theory should first consider whether its assumptions are satisfied, and that a tie’s strength and bridgeness should be measured and modeled independently.