bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiomyocyte-derived circulating extracellular vesicles allow a non-invasive liquid biopsy of myocardium in health and disease
Authors: Spanos Michail , Gokulnath Priyanka , Li Guoping , Hutchins Elizabeth , Meechoovet Bessie , Sheng Quanhu , Chatterjee Emeli , Sharma Ritin , Carnel-Amar Natacha , Lin Claire , Azzam Christopher , Ghaeli Ima , Amancherla Kaushik V , Victorino José Fabian , Garcia-Mansfield Krystine , Pfeffer Ryan , Sahu Parul , Lindman Brian R. , Elmariah Sammy , Gamazon Eric R. , Betti Michael J. , Bledsoe Xavier , Lance Michelle L. , Absi Tarek , Su Yan Ru , Do Ngoc , Contreras Marta Garcia , Varrias Dimitrios , Kladas Michail , Radulovic Miroslav , Tsiachris Dimitris , Spanos Anastasios , Tsioufis Konstantinos , Ellinor Patrick T. , Tucker Nathan R. , Januzzi James L. , Pirrotte Patrick , Talisman Tijana Jovanovic- , Keuren-Jensen Kendall Van , Shah Ravi , Das Saumya 
Published: September 2024

SUMMARYThe ability to track disease without tissue biopsy in patients is a major goal in biology and medicine. Here, we identify and characterize cardiomyocyte-derived extracellular vesicles in circulation (EVs; “cardiovesicles”) through comprehensive studies of induced pluripotent stem cell-derived cardiomyocytes, genetic mouse models, and state-of-the-art mass spectrometry and low-input transcriptomics. These studies identified two markers (POPDC2,CHRNE) enriched on cardiovesicles for biotinylated antibody-based immunocapture. Captured cardiovesicles were enriched in canonical cardiomyocyte transcripts/pathways with distinct profiles based on human disease type (heart failure, myocardial infarction). In paired myocardial tissue-plasma from patients, highly expressed genes in cardiovesicles were largely cardiac-enriched (vs. “bulk” EVs, which were more organ non-specific) with high expression in myocardial tissue by single nuclear RNA-seq, largely in cardiomyocytes. These results demonstrate the first “liquid” biopsy discovery platform to interrogate cardiomyocyte states non-invasively in model systems and in human disease, allowing non-invasive characterization of cardiomyocyte biology for discovery and therapeutic applications.
Correspondence: Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma
Authors: Lim Jing Quan , Huang Dachuan , Ong Choon Kiat 
Published: September 2024

AbstractRecurrent somatic mutation in theECSITgene encoding p.V140A was reported in 19.3% (17/88) extranodal natural-killer/T cell lymphoma (ENKTL) and was associated with hemophagocytic syndrome (HPS). However, another cohort of similar geographical descent also had the ECSITV140Amutation as germline in 20.0% (5/25) of ENKTL and none were somatic. The reanalysis of published data revealed that the reported somatic ECSITV140Acould be germline as well. First-digit analysis on the IDs of sequencing read also found irregularity among the data that initially reported the somatic ECSITV140Amutation. As such, this study questions the only somatic genetic link of HPS in ENKTL and also introduces a simple well-known algorithm to detect data irregularity in voluminous genomic sequencing data.
DNA methylation age acceleration is associated with incident cognitive impairment in the Health and Retirement Study
Authors: Blostein Freida , Bakulski Kelly M. , Fu Mingzhou , Wang Herong , Zawistowski Matthew , Ware Erin B. 
Published: September 2024

AbstractBackgroundDNA methylation clocks have emerged as promising biomarkers for cognitive impairment and dementia. Longitudinal studies exploring the link between DNA methylation clocks and cognitive decline have been constrained by limited sample sizes and a lack of diversity.ObjectiveOur study aimed to investigate the longitudinal associations between DNA methylation clocks and incident cognitive impairment using a larger sample size encompassing a US nationally representative sample from the Health and Retirement Study.MethodsWe measured DNA methylation age acceleration in 2016 by comparing the residuals of DNA methylation clocks, including GrimAge, against chronological age. Cognitive decline was determined by the change in Langa-Weir cognition status from 2016 to 2018. Using multivariable logistic regression, we evaluated the link between DNA methylation age acceleration and cognitive decline, adjusting for cell-type proportions, demographic, and health factors. We also conducted an inverse probability weighting analysis to address potential selection bias from varying loss-to-follow-up rates.ResultsThe analytic sample (N=2,713) at baseline had an average of 68 years old, and during the two years of follow-up, 12% experienced cognitive decline. Participants who experienced cognitive decline during follow-up had higher baseline GrimAge (mean = 1.2 years) acceleration compared to those who maintained normal cognitive function (mean = -0.8 years, p < 0.001). A one-year increase in GrimAge acceleration was associated with 1.05 times higher adjusted and survey-weighted odds of cognitive decline during follow-up (95% CI: 1.01-1.10). This association was consistent after accounting for loss-to-follow-up (OR = 1.07, 95% CI: 1.04-1.11).ConclusionOur study offers insights into DNA methylation age acceleration associated with cognitive decline, suggesting avenues for improved prevention, diagnosis, and treatment.
Severe Mitral Regurgitation in Paradoxical Low-Flow Low-Gradient Severe Aortic Stenosis
Authors: Dahan Shani , Dal-Bianco Jacob , Plakht Ygal , Namasivayam Mayooran , Capoulade Romain , Zeng Xin , Passeri Jonathan J , Yucel Evin , Picard Michael H , Levine Robert A , Hung Judy 
Published: September 2024

AbstractBackgroundPatients with paradoxical low-flow, low-gradient severe aortic stenosis (LFLGAS) exhibit low transvalvular flow rate (Q), while maintaining preserved left ventricular ejection fraction (LVEF). Concomitant severe mitral regurgitation (MR) contributes to the low flow state, adding complexity to diagnosis and management. This study aimed to examine the impact of severe MR on outcomes in paradoxical LFLGAS.MethodsData from an institutional echo database identified 1,189 patients with adjudicated severe aortic stenosis (AVA≤1.0 cm2), low transaortic gradients (mean gradient<40 mmHg), preserved LVEF (≥50%), and low flow rate (Q≤210 ml/sec), to confirm paradoxical LFLGAS. Subgroups were based on MR severity (severe and non-severe). Clinical outcomes included all-cause mortality, aortic valve replacement (AVR), heart failure hospitalizations, and a composite outcome.ResultsIn the severe MR group (n=80), patients had lower flow rates, increased LV dimensions and a more eccentric hypertrophy pattern compared to non-severe MR (n=1,109). Over a median 5-year follow-up, severe MR correlated with higher all-cause mortality (p=0.02) and AVR rates (p=0.012). After adjustment, severe MR was independently associated with increased all-cause mortality risk (HR=1.43, p=0.011) and composite outcome (HR=1.64, p<0.001). AVR significantly reduced mortality at every MR degree, with the most substantial impact in severe MR (HR=0.18, p<0.001). Propensity-adjusted models demonstrated a stronger AVR impact with increasing MR degree (p-for-interaction=0.044).ConclusionsSevere MR in paradoxical LFLGAS is associated with adverse outcomes and distinctive LV remodeling. Aortic valve replacement improves survival across all MR grades, with greater impact in severe MR.
Study Protocol. The CASPAR study protocol. Can cervical stiffness predict successful vaginal delivery after induction of labour? A feasibility, cohort study
Authors: Medford Elizabeth , Lane Steven , Merriel Abi , Sharp Andrew , Care Angharad 
Published: September 2024

AbstractBackgroundInduction of labour (IOL) is a common obstetric intervention in the UK, affecting up to 33% of deliveries. IOL aims to achieve a vaginal delivery prior to spontaneous onset of labour to prevent harm from ongoing pregnancy complications and is known to prevent stillbirths and reduce neonatal intensive care unit admissions. However, IOL doesn’t come without risk and overall, 20% of mothers having an induction will still require a caesarean section birth and in primiparous mothers this rate is even higher.There is no reliable predictive bedside tool available in clinical practice to predict which patient’s undergoing the IOL process will result in a vaginal birth; the fundamental aim of the IOL process. The Bishop’s Score (BS) remains in routine clinical practice as the examination tool to assess the cervix prior to IOL, despite it being proven to be ineffective as a predictive tool and largely subjective. This study will assess the use of the Pregnolia System, a new objective antenatal test of cervical stiffness. This study will explore its’ potential for pre-induction cervical assessment and indication of delivery outcome following IOL.MethodsCASPAR is a feasibility study of term, primiparous women with singleton pregnancies undergoing IOL. Cervical stiffness will be assessed using the Pregnolia System; a novel, non-invasive, licensed, CE-marked, aspiration-based device proven to provide objective, quantitative cervical stiffness measurements represented as the Cervical Stiffness Index (CSI, in mbar). A measurement is obtained by applying the sterile single-use Pregnolia Probe directly to the anterior lip of the cervix, visualised via placement of a speculum.Following informed consent, CASPAR study participants will undergo the Pregnolia System cervical stiffness assessment prior to their IOL process commencing. Participant questionnaires will evaluate the acceptability of this assessment tool in this population. This study will directly compare this novel antenatal test to the current BS for both patient experience of the different cervical assessment tools and for IOL outcome prediction.DiscussionThis feasibility study will explore the use of this novel device in clinical practice for pre-induction cervical assessment and delivery outcome prediction. Our findings will provide novel data that could be instrumental in transforming clinical practice surrounding IOL. Determining recruitment rates and acceptability of this new assessment tool in this population will inform design of a further powered study using the Pregnolia System as the point-of-care, bedside cervical assessment tool within an IOL prediction model.Study registrationThis study is sponsored by The University of Liverpool and registered at ClinicalTrials.gov, identifierNCT05981469, date of registration 7thJuly 2023.
Effect of non-drug Intervention on Cognitive Function and Blood Sugar Control of Type 2 Diabetes patients with Mild Cognitive Impairment: A Meta-analysis Protocol
Authors: Wenhao Su , Hairong Jia , Yanru Wang , Luo Yang , Xueling Li , Jiaqi Zhang , Zhaoyang Wei , Pepertual Tsikwa 
Published: September 2024

AbstractThis study aims to assess non-drug therapies’ safety and effectiveness on cognitive function and blood glucose control of type 2 diabetes with mild cognitive impairment (T2DM-MCI) patients in randomized controlled trials by meta-analysis, providing constructive evidence for non-drug treatment decision-making. The system review plan will strictly follow the Systematic Review and Meta-Analysis Protocols entry for reporting. PubMed, EMBASE, Cochrane Library, CINAHL, Web of Science, CNKI, WANGFANG Database, and SinoMed will be systematically searched with Chinese and English language restrictions, and all randomized controlled trials comparing non-drug treatment with usual care or no intervention or placebo to study cognitive impairment in T2DM-MCI patients will be included. We will also manually search for cited literature. Our primary outcomes are cognitive function and blood sugar control. The risk of bias will be assessed for all studies using the Cochrane risk-of-bias tool (RoB 2) for Systematic Review of Intervention-version 5.1.0. Where possible, meta-analysis using random-effects models will be performed, otherwise, a qualitative summary will be provided. We will adhere to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA-P) guidelines. This meta-analysis compares the efficacy of non-drug interventions for mild cognitive impairment in type 2 diabetes mellitus. It will provide reliable evidence for patients, clinicians, and researchers.The methodological protocol was in registered in the PROSPERO (CRD 42024496248).
Construction of the index system for screening and assessment of dysphagia in Chinese elderly people based on Delphi method
Authors: Gaoqiang Li , Yue Chen , Qinge Yong 
Published: September 2024

AbstractBackgroundDysphagia is an important factor affecting aspiration pneumonia in the elderly, which will greatly increase the risk of poor prognosis and even death. Early detection, diagnosis and effective prevention are the key to improve the prognosis of patients. However, there is currently no systematic tool for screening and evaluating swallowing disorders in the elderly.ObjectiveThis study aimed to establish an index system for the screening and evaluation of dysphagia in the elderly, and to provide evidence for the screening and evaluation of dysphagia in the community and clinic.MethodsThe draft of the index system was determined based on the combination of literature review and clinical practice. The Delphi method was applied to conduct expert correspondence consultation, and the index system for screening and evaluation of dysphagia in the elderly was established. The weight of each index was determined by analytic hierarchy process.ResultsA total of 19 experts in related fields were consulted for 3 rounds. The questionnaire recovery rates were 94.7%, 100% and 100%, respectively. 17 (89.5%), 14 (73.7%) and 5 (26.3%) experts put forward modification opinions, respectively. The expert authority coefficient was 0.920, and the Kendall harmony coefficient was 0.219, 0.261 and 0.306, respectively, with statistical significance (P < 0.001). Finally, the index system for the screening and evaluation of dysphagia in the elderly includes 3 first-level indicators, 10 second-level indicators and 26 third-level indicators.ConclusionThe experts in this study are highly motivated and authoritative, and the established index system for the screening and evaluation of dysphagia in the elderly is scientific, reasonable and targeted, which can provide reference for the screening and evaluation of dysphagia in the community and clinical elderly patients.
Epidemiological pattern of trauma among children 0 - 9 years in Cameroon
Authors: Kibu Odette , Maqungo Sithombo , Nguefack-Tsague Georges , Maqungo Sithombo , Nsagha Dickson Shey , Dissak Delon Fanny N. , Touko Darwin , Tanue Elvis Asangbeng , Oke Rasheedat , McCoy Sandra I. , Christie Sabrinah Ariane , Juillard Catherine , Mefire Alain Chichom 
Published: September 2024

AbstractBackgroundIn low- and middle-income countries, trauma is the leading cause of death among youth and it is also a major cause of disability. Globally, more than 1,600 children and adolescents below the age of 19-years die every day from preventable injuries. Traffic-related injuries, falls, sports-related injuries, assaults, burns, and drownings are the most commonly reported causes of traumatic mortality among children. The mechanism of injury is always diverse in different contexts due to the differences in social determinants of health.ObjectivesTo determine the epidemiological pattern of trauma among children 0 – 9 years in Cameroon.MethodsThis is a retrospective analysis of prospectively collected data from the Cameroon Trauma Registry currently running in 10 pilot sites across seven of the ten regions of Cameroon. We retrieved data for all children aged 0 - 9 years from June 2022 to August 2023. Data was analyzed with respect to the demographics, injury characteristics and outcomes.ResultsOf the 5,439 patients captured in the trauma registry, 267 (4.9%) were children aged 0-9 years. Over 50% (152/267) of the patients were males with 35% (93/267) from rural settings. The top injury mechanism was road traffic injuries (RTI) [137(52.1%)]. These injuries occurred on the streets [142(53.4%)] during leisure activities [205(78.5%)]. Majority of children [104(39%)] involved in RTI injuries were pedestrians and no prehospital care offered to 216(82.8%) of injured children. A total of 39 (16.9%) were discharged with major disability, 111 (48.1%) had limited ability to move and 5 (2.2%) demised or succumbed to their injuries. There was a significant association between injury activity and gender (P = 0.006). Unlike the females, majority of the males were discharged with major disability [21 (53.8%)].ConclusionThis preliminary analysis highlights the burden of trauma among children aged 0 – 9 years and its contribution to the proportion of disabled persons in Cameroon. Leisure activities on the streets increased the number of pediatric injuries especially among males. It is therefore imperative to put in place or reinforce environmental interventions to reduce the burden of pediatric injuries.
Telehealth during and beyond the COVID-19 Pandemic: Evidence from Licensed Dietitians in an Emerging Economy
Authors: Assaad Maya , Chamma Nour , Mateev Miroslav , Rizk Rana 
Published: September 2024

ABSTRACTBackgroundThe SARS-Cov-2 pandemic (COVID-19) sudden onset disrupted the direct access to face-to-face nutrition consultation fostering the rapid adoption of Telehealth by dietitians.ObjectiveThis study investigates Telehealth use among Lebanese Licensed Dietitians (LDs) amid COVID-19, in the absence of national Telehealth practical guidelines (TPG), and offers insights on Telehealth application under uncertainties of pandemic event, economic crisis, and destructed infrastructure occurring simultaneously in Lebanon.DesignA cross-sectional study during March 2023, using an anonymous online survey (44 questions), diffused through the Lebanese Order of Dietitians and social media platforms.ParticipantsThe sample (n=94) consisted mostly of females (98.9%) and young dietitians (mean(SD) age: 30.54(6.41) years) having a mean(SD) of 7.89(5.7) years of experience. Most respondents identified clinical nutrition as their primary practice area (87.2%), mostly in weight management (84%).Main outcome measuresTelehealth experience amid COVID-19 including tools utilized in remote consultations, barriers, facilitators, and perspectives of Telehealth use.Statistical analysesDescriptive analysis (counts, frequencies) using SPSS version 28.ResultsAlthough 48.4% of LDs reported using Telehealth prior COVID-19, this proportion increased to 97.8% during the pandemic. The most common tools used were WhatsApp (90.3%), Zoom (72.0%), and E-mails (41.9%). Reported barriers included bad internet connection (74.2%), patients preferring face-to-face consultation (61.3%), and patients lacking technical literacy (33.3%); benefits included scheduling and time flexibility (83.9%), decrease in practice-related costs (77.4%), and compliance with social distancing measures (53.8%). The majority agreed that Telehealth is needed (78.5%) and applicable in the Lebanese context (64.6%) and expressed the need for Telehealth trainings (78.5%) and TPG for nutrition care (74.2%).ConclusionsThis study recognizes increasing use of Telehealth in Lebanon, warranting the development of Telehealth nutrition care infrastructure comprising national regulations and evidence-based practical guidelines to respond to the innovation in the healthcare industry, and to assure Telehealth sustainability in LDs’ routine practice.
COVID-19 Vaccine effectiveness among Healthcare Workers during the Omicron Period in the country of Georgia, January – June 2022
Authors: Ward Caleb L. , Rojas Castro Madelyn Yiseth , Chakhunashvili Giorgi , Chitaze Nazibrola , Finci Iris , Pebody Richard , Kissling Esther , Katz Mark A. , Sanodze Lia 
Published: September 2024

AbstractIntroductionUnderstanding COVID-19 vaccine effectiveness (VE) in healthcare workers (HCWs) is critical to inform vaccination policies. We measured COVID-19 VE against laboratory-confirmed symptomatic infection in HCWs in the country of Georgia from January – June 2022, during a period of Omicron circulation.MethodsWe conducted a cohort study of HCWs in six hospitals in Georgia. HCWs were enrolled in early 2021. Participants completed weekly symptom questionnaires. Symptomatic HCWs were tested by RT-PCR and/or rapid antigen test (RAT). Participants were also routinely tested, at varying frequencies during the study period, for SARS-CoV-2 by RT-PCR or RAT, regardless of symptoms. Serology was collected quarterly throughout the study and tested by electrochemiluminescence immunoassay for SARS-CoV-2 antibodies. We estimated absolute and relative VE of a first booster dose compared to a primary vaccine series as (1-hazard ratio)*100 using Cox proportional hazards models.ResultsAmong 1253 HCWs, 141 (11%) received a primary vaccine series (PVS) and a first booster, 855 (68%) received PVS only, and 248 (20%) were unvaccinated. Most boosters were BNT162b2 (Comirnaty original monovalent) vaccine (90%) and BIBP-CorV vaccine (Sinopharm) (9%). Most PVS were BNT162b2 vaccine (68%) and BIBP-CorV vaccine (24%). Absolute VE for a first booster was 40% (95% Confidence Interval (CI) -56 – 77) at 7– 29 days following vaccination, -9% (95% CI -104 – 42) at 30 – 59 days, and - 46% (95% CI -156 – 17) at ≥ 60 days. Relative VE of first booster dose compared to PVS was 58% (95% CI 1 – 82) at 7– 29 days following vaccination, 21% (95% CI -33 – 54) at 30 – 59 days, and -9% (95% CI -82 – 34) at ≥ 60 days.ConclusionIn Georgia, first booster dose VE against symptomatic SARS-CoV-2 infection among HCWs was moderately effective but waned very quickly during Omicron. Increased efforts to vaccinate priority groups in Georgia, such as healthcare workers, prior to periods of anticipated high COVID-19 incidence are essential.