bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth
Authors: Xia Tian , Meng Lian , Xu Guixuan , Sun Hao , Chen Hao 
Published: October 2024

AbstractEsophageal squamous cell carcinoma (ESCC) is a common fatal malignant tumor of the digestive tract; however, its pathogenic mechanism is unknown and lacks specific molecular diagnosis and treatment. Therefore, it is particularly important to identify new tumor biomarkers to enhance the early diagnosis and molecular-targeted therapy of ESCC. Here, we found that the E3 ubiquitin ligase Tripartitemotif-containing33 (TRIM33) is highly expressed in ESCC tissues and cell lines, and is associated with adverse clinical outcomes. We determined that TRIM33 drives aerobic glycolysis to promote tumor growth in vivo and in vitro. In terms of mechanism, TRIM33 binds to p53 to inhibit its stability and promote the expression of downstream glycolysis target genes GLUT1, HK2, PKM2, and LDHA. In addition, TRIM33 promotes the polyubiquitination of P53 K48-linked and proteasome degradation. Further studies have shown that the K351 site of P53 is the key site mediating the ubiquitination of P53 K48-linked to promote aerobic glycolysis in ESCC and tumor cell growth. Our results reveal that the TRIM33-P53 signal axis regulates glycolysis during ESCC and may provide a new perspective for the diagnosis and treatment of ESCC.
A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival
Authors: Abassah-Oppong Samuel , Zoia Matteo , Mannion Brandon J. , Rouco Raquel , Tissières Virginie , Spurrell Cailyn H. , Roland Virginia , Darbellay Fabrice , Itum Anja , Gamart Julie , Festa-Daroux Tabitha A. , Sullivan Carly S. , Kosicki Michael , Rodríguez-Carballo Eddie , Fukuda-Yuzawa Yoko , Hunter Riana D. , Novak Catherine S. , Plajzer-Frick Ingrid , Tran Stella , Akiyama Jennifer A. , Dickel Diane E. , Lopez-Rios Javier , Barozzi Iros , Andrey Guillaume , Visel Axel , Pennacchio Len A. , Cobb John , Osterwalder Marco 
Published: October 2024

AbstractApproximately a quarter of the human genome consists of gene deserts, large regions devoid of genes often located adjacent to developmental genes and thought to contribute to their regulation. However, defining the regulatory functions embedded within these deserts is challenging due to their large size. Here, we explore the cis-regulatory architecture of a gene desert flanking the Shox2 gene, which encodes a transcription factor indispensable for proximal limb, craniofacial, and cardiac pacemaker development. We identify the gene desert as a regulatory hub containing more than 15 distinct enhancers recapitulating anatomical subdomains of Shox2 expression. Ablation of the gene desert leads to embryonic lethality due to Shox2 depletion in the cardiac sinus venosus, caused in part by the loss of a specific distal enhancer. The gene desert is also required for stylopod morphogenesis, mediated via distributed proximal limb enhancers. In summary, our study establishes a multi-layered role of the Shox2 gene desert in orchestrating pleiotropic developmental expression through modular arrangement and coordinated dynamics of tissue-specific enhancers.
NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
Authors: Mougios Nikolaos , Cotroneo Elena R. , Imse Nils , Setzke Jonas , Rizzoli Silvio O. , Simeth Nadja A. , Tsukanov Roman , Opazo Felipe 
Published: October 2024

AbstractFluorescence microscopy has long been a transformative technique in biological sciences. Nevertheless, most implementations are limited to a few targets, which have been revealed using primary antibodies and fluorescently conjugated secondary antibodies. Super-resolution techniques such as Exchange-PAINT and, more recently, SUM-PAINT have increased multiplexing capabilities, but they require specialized equipment, software, and knowledge. To enable multiplexing for any imaging technique in any laboratory, we developed NanoPlex, a streamlined method based on conventional antibodies revealed by engineered secondary nanobodies that allow the selective removal of fluorescence signals. We develop three complementary signal removal strategies: OptoPlex (light-induced), EnzyPlex (enzymatic), and ChemiPlex (chemical). We showcase NanoPlex reaching 21 targets for 3D confocal analyses and 5–8 targets for dSTORM and STED super-resolution imaging. NanoPlex has the potential to revolutionize multi-target fluorescent imaging methods, potentially redefining the multiplexing capabilities of antibody-based assays.
Tandem microplastic degradation and hydrogen production by hierarchical carbon nitride-supported single-atom iron catalysts
Authors: Lin Jingkai , Hu Kunsheng , Wang Yantao , Tian Wenjie , Hall Tony , Duan Xiaoguang , Sun Hongqi , Zhang Huayang , Cortés Emiliano , Wang Shaobin 
Published: October 2024

AbstractMicroplastic pollution, an emerging environmental issue, poses significant threats to aquatic ecosystems and human health. In tackling microplastic pollution and advancing green hydrogen production, this study reveals a tandem catalytic microplastic degradation-hydrogen evolution reaction (MPD-HER) process using hierarchical porous carbon nitride-supported single-atom iron catalysts (FeSA-hCN). Through hydrothermal-assisted Fenton-like reactions, we accomplish near-total ultrahigh-molecular-weight-polyethylene degradation into C3-C20 organics with 64% selectivity of carboxylic acid under neutral pH, a leap beyond current capabilities in efficiency, selectivity, eco-friendliness, and stability over six cycles. The system demonstrates versatility by degrading various daily-use plastics across different aquatic settings. The mixture of FeSA-hCN and plastic degradation products further achieves a hydrogen evolution of 42 μmol h‒1 under illumination, outperforming most existing plastic photoreforming methods. This tandem MPD-HER process not only provides a scalable and economically feasible strategy to combat plastic pollution but also contributes to the hydrogen economy, with far-reaching implications for global sustainability initiatives.
Transplacental SARS-CoV-2 protein ORF8 binds to complement C1q to trigger fetal inflammation
Authors: Azamor Tamiris , Familiar-Macedo Débora , Salem Gielenny M , Onwubueke Chineme , Melano Ivonne , Bian Lu , Vasconcelos Zilton , Nielsen-Saines Karin , Wu Xianfang , Jung Jae U , Lin Feng , Goje Oluwatosin , Chien Edward , Gordon Steve , Foster Charles B , Aly Hany , Farrell Ruth M , Chen Weiqiang , Foo Suan-Sin 
Published: October 2024

AbstractPrenatal SARS-CoV-2 infection is associated with higher rates of pregnancy and birth complications, despite that vertical transmission rates are thought to be low. Here, multi-omics analyses of human placental tissues, cord tissues/plasma, and amniotic fluid from 23 COVID-19 mother-infant pairs revealed robust inflammatory responses in both maternal and fetal compartments. Pronounced expression of complement proteins (C1q, C3, C3b, C4, C5) and inflammatory cytokines (TNF, IL-1α, and IL-17A/E) was detected in the fetal compartment of COVID-19-affected pregnancies. While ~26% of fetal tissues were positive for SARS-CoV-2 RNA, more than 60% of fetal tissues contained SARS-CoV-2 ORF8 proteins, suggesting transplacental transfer of this viral accessory protein. ORF8-positive fetal compartments exhibited increased inflammation and complement activation compared to ORF8-negative COVID-19 pregnancies. In human placental trophoblasts in vitro, exogenous ORF8 exposure resulted in complement activation and inflammatory responses. Co-immunoprecipitation analysis demonstrated that ORF8 binds to C1q specifically by interacting with a 15-peptide region on ORF8 (C37-A51) and the globular domain of C1q subunit A. In conclusion, an ORF8-C1q-dependent complement activation pathway was identified in COVID-19-affected pregnancies, likely contributing to fetal inflammation independently of fetal virus exposure.
A data‐driven transient stability‐based approach for out‐of‐step prediction in power systems
Authors: Shazdeh Sirwan , Golpîra Hêmin , Bevrani Hassan 
Published: October 2024

AbstractThis paper presents a prediction‐based algorithm designed to address out‐of‐step (OOS) conditions in power systems. The algorithm utilizes generator data obtained from phasor measurement units. The transient stability of a multi‐machine power system is evaluated using the equal‐area criterion (EAC). The proposed algorithm calculates the characteristics of the P‐δ curves within the EAC framework after a large disturbance. The critical P‐δ trace is determined by analysing the cumulative energy in the acceleration area following fault clearance. The stability margin of the rotor angle is then computed based on the actual active power and its relationship with the critical curve. The algorithm predicts the occurrence of OOS by comparing the measured active power with the corresponding value on the critical curve. Furthermore, a complementary strategy is proposed to predict the OOS condition in integrated inverter‐based power systems. The effectiveness of the proposed algorithm is validated through simulations conducted on the 73‐bus IEEE test power system.
MultiScale spectral–spatial convolutional transformer for hyperspectral image classification
Authors: Gong Zhiqiang , Zhou Xian , Yao Wen 
Published: October 2024

AbstractDue to the powerful ability in capturing the global information, transformer has become an alternative architecture of CNNs for hyperspectral image classification. However, general transformer mainly considers the global spectral information while ignores the multiscale spatial information of the hyperspectral image. In this paper, we propose a multiscale spectral–spatial convolutional transformer (MultiFormer) for hyperspectral image classification. First, the developed method utilizes multiscale spatial patches as tokens to formulate the spatial transformer and generates multiscale spatial representation of each band in each pixel. Second, the spatial representation of all the bands in a given pixel are utilized as tokens to formulate the spectral transformer and generate the multiscale spectral–spatial representation of each pixel. Besides, a modified spectral–spatial CAF module is constructed in the MultiFormer to fuse cross‐layer spectral and spatial information. Therefore, the proposed MultiFormer can capture the multiscale spectral–spatial information and provide better performance than most of other architectures for hyperspectral image classification. Experiments are conducted over commonly used real‐world datasets and the comparison results show the superiority of the proposed method.
Primary Prevention of Cancer-Associated Thrombosis: Current Perspectives
Authors: Hart Christina , van Es Nick , Voigtlaender Minna 
Published: October 2024

AbstractOver the past two decades, the incidence of cancer-associated thrombosis (CAT) has increased. It is nowadays a common and often serious complication among patients with cancer. Although medical thromboprophylaxis is recommended for most surgical and nonsurgical cancer patients, it has been infrequently used in ambulatory patients with cancer because of the burden of treatment and concerns about bleeding. However, various risk assessment scores are now available and randomized placebo-controlled trials have established the efficacy of low-molecular-weight heparin or the direct oral Xa inhibitors rivaroxaban and apixaban in ambulatory patients with cancer at high risk of venous thromboembolism (VTE). This review provides an overview of (1) primary thromboprophylaxis in the setting of hospitalized surgical and medical patients, (2) extended thromboprophylaxis after hospital discharge, (3) performance of risk assessment tools for CAT, and (4) primary thromboprophylaxis in ambulatory patients with cancer. The aim is to provide support to physicians in identifying ambulatory patients with cancer at high VTE risk who benefit most from medical thromboprophylaxis according to current recommendations from international guidelines.
Das weibliche Gehirn und Sex
Authors: Durner Verena , Rüger Alexander , Graf Heiko , Zeiss René 
Published: October 2024

ZUSAMMENFASSUNG
Gegenstand und Ziel Diese Übersichtsarbeit fasst wesentliche Befunde bildgebender Untersuchungen mit sexueller bzw. erotischer Stimulation und hormonelle Einflüsse bei Frauen zusammen.
Ergebnisse Im Vergleich zu Männern befassen sich bedeutend weniger bildgebende Studien mit Frauen und deren neurofunktioneller Verarbeitung sexueller Reize. Es zeigen sich heterogene Befunde in neurofunktionellen Korrelaten sexueller Stimulation bei Frauen, die durch unterschiedliche Paradigmen und Studiendesigns bedingt sein könnten. Bei Frauen führen eine Vielzahl erotischer Reize zu neurofunktionellen Aktivierungen in Regionen, die mit sexueller Reaktion in Verbindung gebracht werden. Nennenswert moduliert werden diese durch hormonelle Einflüsse im Rahmen des Menstruationszyklus, hormonelle Kontrazeption und Menopause, wenngleich spezifische Zuweisungen einzelner Hormone auf spezifische neurofunktionelle Aktivierungen noch offenbleiben. Es zeigten sich geschlechtsspezifische Unterschiede in der Hirnaktivität, insbesondere auf visuelle sexuelle Reize. Ein noch wenig untersuchter Prozess ist der weibliche Orgasmus in der Bildgebung.
Schlussfolgerungen und klinische Relevanz Ein vertieftes Verständnis der Sexualität mit einer breiteren Untersuchung von weiblichen Probanden ist für die Entwicklung eines umfassenden neurowissenschaftlichen Modells zur sexuellen Funktion von entscheidender Bedeutung. Dadurch wird ein Verständnis der sexuellen Funktion unabhängig vom Geschlecht oder der sexuellen Orientierung ermöglicht.
Contrast-enhanced endoscopic ultrasound for differential diagnosis of autoimmune pancreatitis: a meta-analysis
Authors: Zhu Shanshan , Cao Xinguang , Nabi Ghulam , Zhang Fangbin , Liu Ping , Zhang Jingwen , Guo Changqing 
Published: October 2024

Abstract
Background and study aims To assess the diagnostic value of contrast-enhanced endoscopic ultrasound (CE-EUS) for autoimmune pancreatitis and other solid pancreatic masses.
Patients and methods A systematic search of PubMed, Embase, and Web of Science was performed from inception to October 2022. We calculated individual and pooled sensitivities and specificities to determine the diagnostic ability of CE-EUS. In addition, we calculated I2 to test for heterogeneity and explored the source of heterogeneity by meta-regression analysis.
Results A total of 472 patients from seven eligible studies were included. The mean sensitivity and specificity of the Bivariate analysis were 0.84 (95% CI 0.71–0.92) and 0.95 (95% CI 0.84–0.99), respectively. The diagnostic advantage ratio was 107.91 (95% confidence interval [CI] 22.22–524.13), and the area under the summary receiver operating characteristics curve was 0.91 (95% CI 0.88–0.93). The overall heterogeneity of the studies is negligible (I2=0, 95% CI 0–100). However, notable heterogeneity was observed in the combined specificity (P <0.01, I2=74.82) and diagnostic odds ratio (P=0.05, I2=51.54). The heterogeneity in these aspects could be elucidated through sensitivity analysis.
Conclusions Our analysis showed that CE-EUS is useful in identifying autoimmune pancreatitis. However, further large sample size, multicenter, prospective studies are needed to demonstrate its utility.
Future frequencies of coastal floods in Australia: a seamless approach and dataset for visualising local impacts and informing adaptation
Authors: Hague Ben S. , Jakob Dörte , Kirezci Ebru , Jones David A. , Cherny Ilana L. , Stephens Scott A. 
Published: October 2024

The rise of pathways-based approaches to coastal adaptation in Australia has changed user requirements for coastal flood hazard information to support decision-making. This study identifies and addresses three aspects not considered in the existing Australia-specific scientific guidance for planning adaptation to sea-level rise. First, changes in the frequency of present-day extreme sea levels are compared between locations. Second, extreme sea levels are related to impact-based thresholds associated with past flood events. Third, the potential for chronic flooding emerging is assessed. This complements global studies that provide some Australian results on these topics. We survey these to identify the methods most suitable for our application and apply the chosen methods to the reference dataset for monitoring Australian coastal sea-level change. This yields a water-level frequency dataset covering daily to centennial water levels for 37 Australian tide gauges. We analyse the dataset to provide a national picture of how sea-level rise is expected to influence the future frequencies of coastal floods in Australia. For example, 85% of Australian locations expect present-day centennial extremes to occur 30 days per year with less than 1-m sea-level rise. The locations with the largest increases in the future frequency of these extremes have the smallest present-day sea-level extreme magnitudes relative to mean sea level, and lower flood thresholds relative to these extremes. We demonstrate three further potential applications of our dataset and methods using local case studies: impact-based forecasting, climate risk services and identifying the required sea-level rise for adaptation triggers and thresholds to be reached.
Boundary layer height above the Great Barrier Reef studied using drone and Mini-Micropulse LiDAR measurements
Authors: Ryan Robert G. , Eckert Christian , Kelaher Brendan P. , Harrison Daniel P. , Schofield Robyn 
Published: October 2024

The planetary boundary layer height (PBLH) is an important meteorological feature defining the boundary between surface processes and the free troposphere. The PBLH plays a key role in cloud formation and the vertical extent of aerosols and air pollutants. Measurements of PBLH were made by meteorological sensors mounted to a multi-copter drone over the southern Great Barrier Reef, Australia. We then compared these drone-based measurements to remote-sensed PBLH observations, using a Mini-Micropulse (MP) LiDAR system. Across the measurement campaign (1 March–2 April 2023), the mean PBLH value using the drones was 801 ± 203 m. Using the gradient method for MP LiDAR normalised relative backscatter profiles, the mean PBLH was 811 ± 260 m. Using an ideal MP LiDAR profile fitting method the mean was 912 ± 202 m and using a wavelet covariance transform method the mean was 862 ± 298 m. The boundary layer was consistently well mixed, without convective instability or a strong diurnal PBLH cycle. The three MP LiDAR methods compared well to the drone measurements overall with Pearson’s R correlation coefficients >0.60; however, estimates from the MP LiDAR were typically ~10% higher than from the drone. These results indicate congruence between the backscatter- and thermodynamically derived PBLH at One Tree Island, which is robust to variations in sampling conditions and the choice of MP LiDAR PBLH retrieval method.
Parents’ attitudes and response to their child’s pornography consumption in Australia: cross-sectional survey
Authors: Lim Megan S. C. , Johnson Eleanor R. , Raggatt Michelle H. 
Published: October 2024

Background Parents play an important role in monitoring children’s internet use, shaping their child’s understanding of sex, and are key stakeholders in the development of sexual health education policies. Therefore, we investigated parents’ awareness of and responses to children’s pornography exposure. Methods We invited parents with children aged from 8 to 20 years (n = 1029) to complete an observational cross-sectional online survey about pornography and young people. Results A total of 48% of parents thought that young people view pornography rarely or never, and 21% agreed that watching pornography could be part of a healthy relationship for young people. Some parents (27%) were aware that their child had ever viewed pornography. Most parents (56%) acted on this by talking to their child, while others (10%) took no action, or restricted access to devices (6%). Discussing pornography was found to be strongly associated (P < 0.001) with being aware of previous exposure to pornography for the child, and older child age (13 years or older). Parents were more likely to support education as the best practice to address pornography (65% agreed) than restricting access (39% agreed). Conclusions These findings provide new insights for policy responses and can guide the development of tools to support parents to respond to their children’s pornography exposure.
Measuring psychological immersion through cardiovascular response
measures in subtitled films
Authors: González César , Jankowska Anna 
Published: October 2024

Abstract
This paper presents a conceptual replication of Kruger et al.’s (2017a) study,
investigating the utility of heart rate (HR) and heart rate variability (HRV)
indicators as objective measurements of psychological immersion in subtitled
audiovisual products. The study employed a quasi-experimental design in ten
participants viewing video clips with and without subtitles, while their
physiological responses were monitored. Results suggest lower arousal levels in
both conditions compared to baseline, with statistically significant differences
observed in mean HR, mean RR, and RMSSD for the subtitles condition. While most
inferential tests did not yield significant outcomes, descriptive trends
indicate decreased arousal relative to the baseline. They also show increased
arousal throughout the clip for most indicators and opposing trends for Mean HR,
RMSSD and LF/HF between the two conditions. Methodological suggestions include
using multiple data points for HR and HRV analysis, exploring ultra short-term
measurements, selecting self-contained clips for viewing, and considering
post-task baselines for a more accurate resting state representation. Despite
limitations due to the small sample size, this study underscores the potential
of HR and HRV as measures of immersion in translated audiovisual products,
emphasising the need for larger sample sizes in future research to enhance
statistical power and generalizability.