bluuebunny/crossref_metadata_2025_split huggingface dataset:
RELATIONSHIP BETWEEN TRPM4 RS8104571 GENOTYPE, CIRCULATING TRPM4 AND SUR1, AND CLINICAL OUTCOME FOLLOWING TRAUMATIC BRAIN INJURY
Authors: Krocker Joseph D. , Cotton Madeline E. , Ashley Janet R. , Schriner Jacob B. , Osborn Baron K. , Wang Yao-Wei Willa , Cox Charles S. , Wade Charles E. 
Published: September 2024

ABSTRACT
The variant single nucleotide polymorphism rs8104571 has been associated with poor outcomes following traumatic brain injury (TBI) and is most prevalent in those of African ancestry. This single nucleotide polymorphism (SNP) resides within a gene coding for the TRPM4 protein, which complexes with SUR1 protein to create a transmembrane ion channel and is believed to contribute to cellular swelling and cell death in neurological tissue. Our study evaluates the relationship between circulating TRPM4 and SUR1, rs8104571 genotype, and clinical outcome in TBI patients. Trauma patients with moderate to severe TBI were included in this retrospective study. rs8104571 genotyping and admission plasma TRPM4 and SUR1 quantification were performed with real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Adequate plasma for TRPM4 and SUR1 ELISA quantification was available for 289 patients, 54 of whom were African American (AA). Plasma TRPM4 concentration was increased in those with a variant rs8104571 allele compared with wild type when controlling for demographics and injury characteristics in the overall cohort (P = 0.04) and within the AA subgroup (P = 0.01). There was no significant association between plasma TRPM4 or SUR1 and clinical outcome (each P > 0.05). Plasma TRPM4 abundance increased with acute kidney injury severity (P = 0.02). The association between increased plasma TRPM4 and variant rs810457 supports an underlying mechanism involving increased neuroinflammation with a subsequent increase in the leakage of TRPM4 from the central nervous system into circulation. Alternative sources of plasma TRPM4 including the kidney cannot be excluded and may play a significant role in the pathophysiology of trauma as well.
Impact of Chronic Kidney Disease on Outcomes following Vascular Procedure in the Vascular Quality Initiative
Authors: Caron  Elisa , Yadavalli Sai Divya , Manchella Mohit , Jabbour Gabriel , Gomez-Mayorga Jorge L. , Davis Roger B. , Patel Virendra I. , Stone David H. , Conrad Mark F. , Schermerhorn Marc L. 
Published: September 2024

Background:
Chronic kidney disease (CKD) increases morbidity and mortality in most vascular procedures. However, a binary classification of estimated glomerular filtration rate (eGFR) <60 mL/min/1.73 m2, which is often used in both modeling and clinical trials, may not be optimal for predicting clinical outcomes.

Objective:
Determine the optimal eGFR cutoff for use in risk stratification and prediction models.

Methods:
Vascular Quality Initiative (VQI) data for non-emergent, first-time OAR, EVAR, TEVAR, CEA, CAS, PVI, Supra- and infra-inguinal bypass were analyzed from to 2013-2023 and divided into cohorts based on eGFR (≥60, 45-59, 30-44, <30, and preoperative dialysis). χ2 and logistic regression were used to evaluate perioperative outcomes.

Results:
Compared to patients with eGFR ≥60, those with eGFR 45-59 had similar odds of mortality following all procedures, except TEVAR. Driven by this group, the combined cohort showed a slight increase in the odds of mortality for eGFR 45-59 (0.6% vs. 0.7%, aOR 1.16, P=0.002). Those in the 30-44 group demonstrated increased odds of mortality both overall and in the individual procedure groups (0.6% vs. 1.2%, aOR 1.78, P<0.001). The odds of mortality continued to increase with worsening eGFR. The overall rate of new permanent dialysis was low for all eGFR cohorts, with a 0.02% difference between those with eGFR >60 and those in the 45-59 cohort (0.04% vs. 0.06%; a OR 1.65, P<0.001). The odds of permanent dialysis likewise continued to increase with decreasing eGFR.

Conclusions
Rather than a binary eGFR cutoff of ≥60 and <60 to stratify patient risk, better risk stratification may be achieved by using five groups of ≥60, 45-59, 30-44, <30, and preoperative dialysis.
Are trauma centers penalized for improved prehospital resuscitation?: The effect of prehospital transfusion on arrival vitals and predicted mortality
Authors: Clements Thomas W. , Van Gent Jan-Michael , Kaminski Carter , Wandling Michael W. , Moore Laura J. , Cotton Bryan A. 
Published: September 2024

BACKGROUNDPrediction models for survival in trauma rely on arrival vital signs to generate survival probabilities. Hospitals are benchmarked on expected and observed outcomes. Prehospital blood (PB) transfusion has been shown to improve mortality, which may affect survival prediction modeling. We hypothesize that the use of PB increases the predicted survival derived from probability models compared with non–blood-based resuscitation.METHODSAll adult trauma patients presenting to a level 1 trauma center requiring emergency release blood transfusion from January 2017 to December 2021 were reviewed. Patients were grouped into those receiving PB or those who did not (no PB). Prehospital Trauma and Injury Severity Score (TRISS) and shock index were compared with those at presentation to hospital. Univariate and multivariate regressions were performed to identify factors associated with changes in survival probability at presentation.RESULTSIn total, 2117 patients were reviewed (PB, 1,011; no PB, 1,106). Patients receiving PB were younger (35 vs. 40 years,p< 0.001), more likely to have blunt mechanism (71% vs. 65%,p= 0.002), and more severely injured (Injury Severity Score, 27 vs. 25;p< 0.001) and had higher rates of prehospital hypotension (44% vs. 19%,p< 0.001) and shock index (1.10 vs. 0.87,p< 0.001). Upon arrival, PB patients had lower rates of ED hypotension (34% vs. 39%,p= 0.01), and significant improvements in arrival TRISS scores (+0.09 vs. −0.02,p< 0.001) and shock index (+0.10 vs. −0.07,p< 0.001) compared with prehospital. On multivariate analysis, PB was associated with a threefold increase in unexpected survivors (odds ratio, 3.28; 95% confidence interval, 2.23–4.60).CONCLUSIONThe use of PB was associated with improved probability of survival and an increase in unexpected survivors. Applying TRISS and shock index at hospital arrival does not account for en route hemostatic resuscitation, causing patients to arrive with improved vitals despite severity of injury. Caution should be used when implementing survival probability calculations using arrival vitals in centers with prehospital transfusion capability.LEVEL OF EVIDENCETherapeutic/Care Management; Level IV.
Etiologies and Outcomes of Granulomatosis With Polyangiitis-Associated Optic Neuropathy: A Case Series and Review of the Literature
Authors: Salman Abdul-Rahman , Hur Minjun , Warrington Kenneth J. , Garrity James A. , Koster Matthew J. , Chodnicki Kevin D. , Tajfirouz Deena A. , Chen John J. 
Published: September 2024

Background:
Granulomatosis with polyangiitis (GPA), formerly known as Wegener granulomatosis, is a rare autoimmune disease characterized by inflammation of small- to medium-sized blood vessels (vasculitis). We described the 3 causes of GPA-associated optic neuropathy (compressive, inflammatory, or ischemic) and analyzed initial and final visual acuities (VAs) in each group, which could potentially help prognosticate visual outcomes depending on the etiology of optic neuropathy.

Methods:
This was a retrospective chart review of patients who were diagnosed with GPA-associated optic neuropathy and were seen in the Department of Ophthalmology at Mayo Clinic in Rochester, Minnesota. Only patients who met the 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology GPA classification criteria were included.

Results:
A total of 12 patients with GPA-associated optic neuropathy were identified. The etiology of the optic neuropathy was compressive in 6 patients, inflammatory in 4 patients, and ischemic in 3 patients. One patient had compressive optic neuropathy initially, then presented with GPA-related optic neuritis years later. Four patients in the compressive optic neuropathy group had orbital masses requiring orbitotomy for debulking, and the remaining 2 patients had compression from pachymeningitis. Average logarithm of the minimum angle of resolution (logMAR) VA at optic neuropathy onset was 1.50, 1.50, and 0.67 (Snellen equivalent 20/600, 20/600, and 20/100, respectively). At the last follow-up, average logMAR VA was 0.91, 1.73, and 1.10 (Snellen equivalent 20/160, 20/1,000, and 20/250, respectively) for each group.

Conclusion:
Visual outcomes were variable, with compressive and inflammatory optic neuropathies showing improvement in 4 eyes and worsening in 3 eyes in total. Patients with ischemic optic neuropathy from GPA were either stable or worsened over the course of the disease.
Structure-guided secretome analysis of gall-forming microbes offers insights into effector diversity and evolution
Authors: Mukhopadhyay Soham , Javed Muhammad Asim , Wu Jiaxu , Pérez-López Edel 
Published: September 2024

ABSTRACTPhytopathogens secrete effector molecules to manipulate host immunity and metabolism. Recent advances in structural genomics have identified fungal effector families whose members adopt similar folds despite sequence divergence, highlighting their importance in virulence and immune evasion. To extend the scope of comparative structure-guided analysis to more evolutionarily distant phytopathogens with similar lifestyles, we used AlphaFold2 to predict the 3D structures of the secretome from selected plasmodiophorid, oomycete, and fungal gall-forming pathogens. Clustering protein folds based on structural homology revealed species-specific expansions and a low abundance of known orphan effector families. We identified novel sequence- unrelated but structurally similar (SUSS) effector clusters, rich in conserved motifs such as ’CCG’ and ’RAYH’. We demonstrate that these motifs likely play a central role in maintaining the overall fold. We also identified a SUSS cluster adopting a nucleoside hydrolase-like fold conserved among various gall-forming microbes. Notably, ankyrin proteins were significantly expanded in gall-forming plasmodiophorids, with most being highly expressed during clubroot disease, suggesting a role in pathogenicity. Altogether, this study advances our understanding of secretome landscapes in gall-forming microbes and provides a valuable resource for broadening structural phylogenomic studies across diverse phytopathogens.
CRISPR-based dissection of miRNA binding sites using isogenic cell lines is hampered by pervasive noise
Authors: Prajapat Mahendra K. , Vidigal Joana A. 
Published: September 2024

ABSTRACTNon-coding regulatory sequences play essential roles in adjusting gene output to cellular needs and are thus critical to animal development and health. Numerous such sequences have been identified in mammalian genomes ranging from transcription factors binding motifs to recognition sites for RNA-binding proteins and non-coding RNAs. The advent of CRISPR has raised the possibility of assigning functionality to individual endogenous regulatory sites by facilitating the generation of isogenic cell lines that differ by a defined set of genetic modifications. Here we investigate the usefulness of this approach to assign function to individual miRNA binding sites. We find that the process of generating isogenic pairs of mammalian cell lines with CRISPR-mediated mutations introduces extensive molecular and phenotypic variability between biological replicates making any attempt of assigning function to the binding site essentially impossible. Our work highlights an important consideration when employing CRISPR editing to characterize non-coding regulatory sequences in cell lines and calls for the development and adoption of alternative strategies to address this question in the future.
Effect of the spatial curvature on light bending and time delay in a curved Einstein-Straus–de Sitter spacetime
Authors: Guenouche Mourad 
Published: September 2024

A method of general applicability has been developed, whereby the null geodesic equations of the Einstein-Straus–de Sitter metric can be integrated simultaneously in terms of the curvature constant k. The purpose is to generalize the computation of light deflection and time delay by a spherical mass distribution. Assuming a flat Universe with most recent measurements of the Hubble constant H0 and the cosmological constant Λ, five time delays between the four bright images of the lensed quasar SDSS J1004+4112 have been forecasted and compared to others in the field, ΔtDC=(3250±64)  days (8.90 yr), ΔtDA=2049−58+59  days (5.61 yr), ΔtAC=(1269±77)  days (3.47 yr), ΔtBC=1176−77+78  days (3.22 yr), and ΔtAB=(93±70)  days. This set of time delays constrains the galaxy cluster mass to be M=(2.447±0.73)×1013M⊙. In addition, we have reviewed the question of the possible contribution of a positive Λ to reduce the light bending, and concluded that the changes are seemingly too small to be appreciable on cosmological scales. The same conclusion has been reached regarding the time delay. Having addressed the question of the effect of the spatial curvature in both closed and open Universe, we have found that the strong lensing is slightly affected by the expected small curvature density Ωk0 of the current Universe within its error bar |Ωk0|⪅0.001, in such a way that it may safely be neglected. However, it is only if Ωk0 gets quite larger that the effect being noticeable. While it is only theoretically possible for Ωk0 to be higher, it is worthwhile to stress that this should impact the light bending and time delay, causing them to decrease or increase depending upon whether the spatial curvature is positive or negative. Furthermore, one can infer that the observed light deflection and time delay independently, which are found to be significantly deviated from those of the flat Universe, may serve as a useful means to provide constraints on Ωk0, thus making the approach employed in this work more promising than others.

Published by the American Physical Society
2024
Examination of personality types as predictors of safety attitudes/behaviours, in support of enhancing safety in healthcare: a scoping review
Authors: Kil Yeojin , Graham Margaret , Chatzi Anna V. 
Published: September 2024

PurposeProvisions for the minimisation of human error are essential through governance structures such as recruitment, human resource allocation and education/training. As predictors of safety attitudes/behaviours, employees’ personality traits (e.g. conscientiousness, sensation-seeking, agreeableness, etc.) have been examined in relation to human error and safety education.Design/methodology/approachThis review aimed to explore research activity on the safety attitudes of healthcare staff and their relationship with the different types of personalities, compared to other complex and highly regulated industries. A scoping review was conducted on five electronic databases on all industrial/work areas from 2001 to July 2023. A total of 60 studies were included in this review.FindingsStudies were categorised as driving/traffic and industrial to draw useful comparisons between healthcare. Certain employees’ personality traits were matched to positive and negative relationships with safety attitudes/behaviours. Results are proposed to be used as a baseline when conducting further relevant research in healthcare.Research limitations/implicationsOnly two studies were identified in the healthcare sector.Originality/valueThe necessity for additional research in healthcare and for comparisons to other complex and highly regulated industries has been established. Safety will be enhanced through healthcare governance through personality-based recruitment, human resource allocation and education/training.
Revisiting Indonesia halal tourism policy in light of GATS
Authors: Ruhaeni Neni , Yusdiansyah Efik , Aqimuddin Eka An 
Published: September 2024

Purpose
As a growth industry in the international tourism segment market, halal tourism domestic policy should align with General Agreement on Trade in Services (GATS) as an international trade in services regulation. This paper aims to examine Indonesia’s halal tourism policy and its intersection with obligations as a member state of the World Trade Organization (WTO). Particularly in balancing Indonesia’s international obligation and the right to regulate.

Design/methodology/approach
The present study uses normative legal research by analysing legal materials, primarily GATS and Indonesia’s halal tourism policy. By using this methodology, this paper seeks the normative ideal domestic approach that aligns with Indonesia’s international obligations within GATS.

Findings
Regional regulations primarily govern the halal tourism policy in Indonesia. The critical substance of the regulation is the mandatory halal certification for the implementation of halal tourism. This obligation may be incompatible with Indonesia’s commitment to liberalise the tourism sector under the GATS. The current legal framework gives rise to a lack of consistency in its application despite its adherence to the halal tourism standards established by the MUI. At the same time, the provincial and regent authorities lacked the authority to do so. The authors argue that halal tourism policy shall be promulgated in the national-level policy to settle this issue. This measure is necessary to mitigate conflicts between prevailing norms and Indonesia’s international commitments within GATS. Therefore, Indonesia can uphold both its international obligations and national interests.

Originality/value
This paper presents a novelty contribution by highlighting the absence of prior research examining Indonesia’s adherence to its international commitments under the GATS in formulating domestic legislation on halal tourism. To close this gap, this study suggests that national legislation governing halal tourism should consider international obligations in the tourism sector under the GATS.
A meta‐analysis of factors influencing the inactivation of Shiga toxin‐producing Escherichia coli O157:H7 in leafy greens
Authors: Owade Joshua Ombaka , Bergholz Teresa M. , Mitchell Jade 
Published: September 2024

AbstractRecent advancements in modeling suggest that microbial inactivation in leafy greens follows a nonlinear pattern, rather than the simple first‐order kinetics. In this study, we evaluated 17 inactivation models commonly used to describe microbial decline and established the conditions that govern microbial survival on leafy greens. Through a systematic review of 65 articles, we extracted 530 datasets to model the fate of Shiga toxin‐producing Escherichia coli O157:H7 on leafy greens. Various factor analysis methods were employed to evaluate the impact of identified conditions on survival metrics. A two‐parameter model (jm2) provided the best fit to most of both natural and antimicrobial‐induced persistence datasets, whereas the one‐parameter exponential model provided the best fit to less than 20% of the datasets. The jm2 model (adjusted R2 = .89) also outperformed the exponential model (adjusted R2 = .58) in fitting the pooled microbial survival data. In the context of survival metrics, the model averaging approach generated higher values than the exponential model for >4 log reduction times (LRTs), suggesting that the exponential model may be overpredicting inactivation at later time points. The random forest technique revealed that temperature and inoculum size were common factors determining inactivation in both natural and antimicrobial‐induced die‐offs.. The findings show the limitations of relying on the first‐order survival metric of 1 LRT and considering nonlinear inactivation in produce safety decision‐making.
Minimalist economic management, deferred revenue regime and aid dependency: Explaining contradictory post‐war statebuilding aims
Authors: Rafi Kambaiz 
Published: September 2024

AbstractThe paper analyses a contradiction in the liberal approach to post‐war statebuilding. The form of the state is seen to aim for the establishment of a centralised maximalist administration when the state's de jure economic policy makes its revenue dependant on market‐generated private sector taxes that are either inadequate or its institutions are part of the reconstruction process. This conflation de facto leads to dependency on official development assistance (ODA), mainly administered through exogenous‐to‐state agencies that undermine the nascent state's bureaucratic development. The paper introduces the concept of deferred revenue regime and argues that dependency on ODA is one empirical symptom of the contradiction in the liberal approach to statebuilding. Using a high‐profile recent example in an instrumental case study, Afghanistan, from 2002 to 2021, the paper develops a diachronic sequencing of significant policy decisions to suggest temporal causality between economic management and ODA dependency, relying on primary data and stylised statistics. The findings contribute to post‐war statebuilding, institutionalism and the political economy of aid.
Incarceration and food insecurity: Challenges and opportunities for museum interpretation
Authors: Fuggle Sophie , McAtackney Laura 
Published: September 2024

AbstractThis article focuses on the role of food interpretation in prison museums and penal heritage sites, which has been underexplored in recent critical analyses of penal tourism and heritage. The authors argue that food is a fundamental part of the lived experience of confinement and detention and lends itself to multiple forms of interpretation and programming activities. Following an overview of existing literature on food insecurity and the different research methods available in recounting stories about food and food insecurity, the article is divided into three main sections. These explore the connections between the built heritage of prisons and the wider landscape, personal and political experiences of hunger, and the potential of art and creativity in negotiating food insecurity. The article concludes with reflections on how food narratives can be further used by prison museums to engage with contemporary issues of social justice, sustainability, decoloniality, and abolition.