bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant effect of leaf extracts from Cressa cretica against oxidation process in soybean oil
Authors: Afshari Afsaneh , Sayyed‐Alangi S. Zahra 
Published: June 2016

AbstractPhenolic compounds from Cressa cretica leaves were extracted using different solvents (water and ethanol 70%) during 3–24 h by immersion method and were determined according to the Folin–Ciocalteu method. The antioxidant activities of the extracts were investigated by 1,1‐diphenyl‐2‐picryl hydrazyl (DPPH) radical scavenging, ferric reduction activity potential and total antioxidant capacity assays compared with synthetic antioxidant namely BHT. The results were shown that the most total phenol content was outcome of the ethanolic extract after 18 h (68.512 ± 0.36 mg gallic acid/g dry extract) extraction. The results of different assays for determination of antioxidant potential of the extracts as well as their EC50 values were indicated antioxidant activities in order: BHT>ethanolic extract>aqueous extract. Also, the antioxidant activities were enhanced with increasing of the extracts and BHT concentrations. The results of Peroxide value (PV) and Thiobarbituric acid (TBA) tests were revealed that the ethanolic extract with various concentrations (200–1000 ppm) were suppressed the oxidation of soybean oil. The highest oxidation inhibitor was belonged to 1000 ppm concentration of the ethanolic extract so that can be good alternative for BHT.
BioActivities of Coturnix japonica (quail) egg yolk and albumen against physiological stress
Authors: Oladipo Gideon O. , Ibukun Emmanuel O. 
Published: June 2016

AbstractCold and immobilization stressors can generate oxidative stress as well as skeletal muscle fatigue. Free radicals cause oxidative degradation of lipids, proteins, nucleic acids, and carbohydrates molecules thereby compromising cell integrity and function. Coturnix japonica (quail) egg had been described as being very functional biochemically, due to the essential biomolecules it contains in very regulated quantity. This study was designed to evaluate the in vitro antioxidant activity of extracts of quail egg yolk and the albumen. The assessment of the antioxidant potentials was typified using the total antioxidant capacity, and ABTS, DPPH (1‐diphenyl‐2‐picrylhydrazyl) and hydroxyl radicals scavenging activities. Others are reducing power, metal chelating and lipid peroxidation inhibition activities. The antistress activities of quail egg yolk and albumen were evaluated on hepatopathic enzymes as well as endogenous antioxidant enzymes. The total antioxidant activities of the yolk extract (YE) and the albumen extracts (AE) were, respectively, 186.57 ± 6.441 mg/g and 172 ± 10.690 mg/g AAE (Ascorbic Acid Equivalent). The YE exhibited significant, potent and appreciable antioxidant activities than AE in a concentration‐dependent manner. The study confirmed that quail egg yolk contained highly antioxidative bioactive compounds not present in albumen, contributing to its (yolk) overall antioxidant and anti‐inflammatory (antistress) properties, thus necessitating their (albumen and yolk) beneficial effects in the management of oxidative and inflammatory conditions.
Excess River Sedimentation at Bridges in the Raya Graben (Northern Ethiopia)
Authors: Demissie Biadgilgn , Billi Paolo , Frankl Amaury , Haile Mitiku , Nyssen Jan 
Published: June 2016

AbstractThis study investigates the temporal variability and effects of bio‐climatic factors and bridges on local hydro‐geomorphic conditions that cause excess sedimentation and flood hazard in ephemeral rivers of the Raya graben (northern Ethiopia). Normalized difference vegetation index (NDVI) was analyzed using Landsat imageries of 1972, 1986, 2000, 2005, 2010, and 2014. Daily rainfall data of three meteorological stations were considered to analyze the temporal trends and return time intervals of rainfall intensity in 24 h. Stream‐bed gradient and bed material grain size were measured in 20 river reaches. Results show that the maximum NDVI values were recorded in the time interval from 2000 to 2014. Nevertheless, during this decade, the study bridges experienced the largest excess sedimentation, in some bridges up to 1·6 m. Different factors to account for it are discussed as no evidence of rainfall pattern, accountable for an increase in sediment delivery from the headwaters, is found. The field data show that channel narrowing at bridges is the main cause of thick upstream sedimentation and of increasing frequency of overbank flows during the last 15 years. A simple hydraulic approach is presented to explain the variability of sediment deposition at bridges and change in bed material grain size between the main stem and the reach right upstream of the bridge. Copyright © 2016 John Wiley & Sons, Ltd.
FOXJ2 controls meiosis during spermatogenesis in male mice
Authors: Miao Hui , Miao Cong‐Xiu , Li Na , Han Jing 
Published: June 2016

SUMMARYSpermatogenesis is a highly complex cell differentiation process necessary for production of haploid spermatozoa. Central to this unique process is spermatocyte meiosis. FOXJ2 (Forkhead box J2), a FOX transcription factor, is specifically expressed in meiotic spermatocytes in adult mouse testes, so we used a germ cell specific conditional knockout model (Foxj2flox/flox, Mvh‐Cre) to explore its role in spermatogenesis. Loss of FOXJ2 in the male germ line led to meiotic arrest and complete infertility. Although, DNA double‐strand breaks (DSBs) were initiated, Foxj2‐deficient spermatocytes failed to form chromosomal synapses and perform DSB repair. Furthermore, Foxj2‐deficient spermatocytes contained significantly less mRNA encoding DSB repair‐associated factors (Rad18, Rad51, Brca1, Brca2, and Tex15) and meiotic arrest‐related proteins (Fzr1, Hsp70‐2, Spata22, Eif4g3, and Zpac); in contrast, no change was observed in the expression of spermatogonia markers (Gfra1, Zbtb16, and c‐Kit) and germ cell markers (Dazl, Mvh, and Tra98). Taken together, FOXJ2 appears to promote meiotic progression in male mice by a mechanism that needs further investigation. Mol. Reprod. Dev. 83: 684–691, 2016 © 2016 Wiley Periodicals, Inc.
Ante Mortem Cranial Trepanation in the Late Bronze Age in Western Siberia
Authors: Slepchenko S. M. , Vybornov A. V. , Slavinsky V. S. , Tsybankov A. A. , Matveev V. E. 
Published: June 2016

AbstractWe report a case study of cranial trepanation in a male subject 30 to 40 years of age from the Nefteprovod II burial ground in the Anzhevsk archeological site. This burial dates back to the Late Bronze Age, in particular the Karasuk culture located in the Minusinsk Basin on the Yenisei River and on the upper reaches of the Ob River. The left parietal bone had an opening with evident signs of bone healing, as well as signs of inflammatory reaction from both bone plates of the calvarium. The strongest signs of inflammation were located around the trepanation opening at the exocranium, suggesting that it occurred after, rather than before, the operation.Although trepanation was the main cause for the development of the changes noted in the preceding texts, there are no reasons to believe that the subject died from complications arising from infection after trepanation. The patient survived and later died for reasons that may never be determined. Medical necessity was the most likely justification for trepanation. Immersion in altered states of consciousness may also have been a necessary part of the trepanation process as a mode of sedation, along with other shamanic practices, such as consumption of psychotropic substances or ecstatic dance. These data, together with reports of other ante mortem burials, raised questions about the application of anaesthesia and possible techniques of cranial trepanation. These issues and possible postoperative complications are discussed in the following text. Copyright © 2016 John Wiley & Sons, Ltd.
Synthesis of novel tri‐benzoxazine and effect of phenolic nucleophiles on its ring‐opening polymerization
Authors: Nalakathu Kolanadiyil Sini , Azechi Motohisa , Endo Takeshi 
Published: June 2016

ABSTRACTA trifunctional benzoxazine, 1,3,5‐tris(3‐phenyl‐3,4‐dihydro‐2H‐benzo[1,3]oxazin‐6‐yl)benzene (T‐Bz) was synthesized and in an effort to reduce its curing temperature (curing maxima at 238 °C), it was mixed with various phenolic nucleophiles such as phenol (PH), p‐methoxy phenol (MPH), 2‐methyl resorcinol (MR), hydroquinone (HQ), pyrogallol (PG), 2‐naphthol (NPH), 2,7‐dihydroxy naphthalene (DHN), and 1,1'‐bi‐2‐naphthol (BINOL). The influence of these phenolic nucleophiles on ring‐opening polymerization temperature of T‐Bz was examined by DSC and FTIR analysis. T‐Bz undergoes a complete ring‐opening addition reaction in the presence of bi‐ and trifunctional phenolic nucleophiles (MR/HQ/PG/DHN) at 140 °C (heated for 3 h) and forms a networked polybenzoxazine (NPBz). The NPBzs showed a high thermal stability with Td20 of 350–465 °C and char yield of 67–78% at 500 °C; however, a diminutive weight loss (6.9–9.8%) was observed at 150–250 °C (Td5: 215–235 °C) due to degradation of phenolic end groups. This article also gives an insight on how the traces of phenolic impurities can alter the thermal properties of pure benzoxazine monomer as well as its corresponding polymer. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2811–2819
Preparation of ureido group bearing polymers and their upper critical solution temperature in water
Authors: Fujihara Ami , Itsuki Kohei , Shimada Naohiko , Maruyama Atsushi , Sagawa Naoya , Shikata Toshiyuki , Yusa Shin‐Ichi 
Published: June 2016

ABSTRACTPoly(2‐ureidoethylmethacrylate) (PUEMn) was synthesized via reversible addition‐fragmentation chain transfer (RAFT) radical polymerization and following polymer reaction. We prepared two PUEMn samples with different degrees of polymerization (n = 100 and 49). The polymers exhibited upper critical solution temperature (UCST) in phosphate‐buffered saline (PBS) solution. The phase separation temperature (Tp) in PBS can be controlled ranging from 17 to 55 °C by changing molecular weight of the polymer, polymer concentration, and adding NaCl concentration. The polymers in PBS formed coacervate drops by liquid–liquid phase separations below Tp. Results of the dielectric relaxation measurement, the hydration number per monomeric unit was 5 above Tp. Based on a fluorescence study, the polymer formed slightly hydrophobic environments below Tp. The liquid–liquid phase separation was occurred presumably because of weak hydrophobic interactions and intermolecularly hydrogen bonding interactions between the pendant ureido groups. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2845–2854
One‐pot, solvent‐free, metal‐free synthesis and UCST‐based purification of poly(ethylene oxide)/poly‐ε‐caprolactone block copolymers
Authors: Ianiro Alessandro , Jiménez‐Pardo Isabel , Esteves A. Catarina C. , Tuinier Remco 
Published: June 2016

ABSTRACTA fast, one pot, solvent‐free and metal‐free synthesis of poly‐ε‐caprolactone and poly(ethylene oxide) block copolymers is reported. Copolymers with different molar mass, different hydrophilic to lipophilic balance, high degree of conversion and narrow molar mass dispersity have been obtained by organocatalyzed ring opening polymerization of ε‐caprolactone in presence of mono‐ or diol‐poly(ethylene oxide) as initiator and fumaric acid as catalyst. A new biocompatible and environmental friendly purification method is presented, exploiting the upper critical solution temperature of these class of copolymers in ethanol. The phase diagrams of the synthesized copolymers in ethanol are also reported. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem.2016,54, 2992–2999
Peptide‐poly(tert‐butyl methacrylate) conjugate into composite micelles in organic solvents versus peptide‐poly(methacrylic acid) conjugate into spherical and worm‐like micelles in water: Synthesis and self‐assembly
Authors: Saha Anupam , Jana Somdeb , Mandal Tarun K. 
Published: June 2016

ABSTRACTPeptide–polymer conjugates are versatile class of biomaterials composed of a peptide block covalently linked with a synthetic polymer block. This report demonstrates the synthesis of peptide‐poly(tert‐butyl methacrylate) (Peptide‐PtBMA) conjugates of varying molecular weights via a “grafting from” atom transfer radical polymerization (ATRP) technique using as‐synthesized peptide‐based initiator in toluene. Peptide‐PtBMA conjugate is soluble in many organic solvents and undergoes self‐assembly into micro/nanospheres in DMF/THF as observed from both FESEM and DLS results. The conjugate micro/nanospheres are nothing but the composite micelles formed by the secondary aggregation of primary micelles generated initially in these organic solvents. The hydrolysis of tert‐butyl groups of Peptide‐PtBMA conjugate leads to the formation of peptide‐poly(methacrylic acid) (Peptide‐PMA) conjugate. The circular dichroism (CD) analysis exhibits the presence of β‐sheet conformation of peptide moiety in synthesized conjugates. The formed Peptide‐PMA conjugate is soluble in water and owing to its amphiphilic character, the conjugate molecules self‐assemble into spherical micelles as well as worm‐like micelles upon increasing the concentration of conjugate in water. However, the sodium salt of Peptide‐PMA conjugates (Peptide‐PMAS) self‐assembles into only spherical swollen micelles in water at higher (pH ∼10). The critical aggregation concentrations (CACs) of both Peptide‐PMA and Peptide‐PMAS micelles are measured by fluorescence spectroscopy. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 3019–3031
Cancer beliefs and patient activation in a diverse, multilingual primary care sample
Authors: Hay Jennifer L. , Zabor Emily C. , Kumar Julie , Brennessel Debra , Kemeny Margaret M. , Lubetkin Erica I. 
Published: June 2016

AbstractObjectivesGreater patient activation, defined as having the knowledge, skills, and confidence to manage one's health, is associated with cancer control behaviors. Cancer risk beliefs may be associated with patient activation, and delineating this relationship could inform cancer control interventions across diverse patient subgroups. This study examines associations between cancer risk beliefs, language preference, and patient activation within a multilingual urban primary care setting.DesignPatients 18 years and older within a New York City public hospital serving a large proportion of non–native‐born Americans were surveyed regarding their cancer risk beliefs and patient activation in Haitian Creole, Spanish, or English based on language preference during a health care visit.ResultsThe sample (N = 460) included 150 Haitian Creole speakers, 159 Spanish speakers, and 151 English speakers and was primarily non‐White (92%). Most participants (84%) had not been born in the United States. Cancer risk beliefs differed across language preference. Beliefs that cancer could be avoided by minimizing thoughts about cancer risk were significantly higher in Haitian Creole speakers than in others; reported negative emotion when thinking about cancer risk was higher in Spanish and English than in Haitian Creole speakers. These cancer risk beliefs were positively related to patient activation, even when controlling for language preference.ConclusionCancer risk beliefs differ across language preference and are related to patient activation, making them potentially important in cancer control. Consideration of language represents important demographic stratification for understanding the frequency and relevance of different beliefs about cancer and patient activation.
Potential DNA binding and nuclease functions of ComEC domains characterized in silico
Authors: Baker James A. , Simkovic Felix , Taylor Helen M.C. , Rigden Daniel J. 
Published: June 2016

ABSTRACTBacterial competence, which can be natural or induced, allows the uptake of exogenous double stranded DNA (dsDNA) into a competent bacterium. This process is known as transformation. A multiprotein assembly binds and processes the dsDNA to import one strand and degrade another yet the underlying molecular mechanisms are relatively poorly understood. Here distant relationships of domains in Competence protein EC (ComEC) of Bacillus subtilis (Uniprot: P39695) were characterized. DNA‐protein interactions were investigated in silico by analyzing models for structural conservation, surface electrostatics and structure‐based DNA binding propensity; and by data‐driven macromolecular docking of DNA to models. Our findings suggest that the DUF4131 domain contains a cryptic DNA‐binding OB fold domain and that the β‐lactamase‐like domain is the hitherto cryptic competence nuclease. Proteins 2016; 84:1431–1442. © 2016 The Authors Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.
The part of a long beta hairpin from the scrapie form of the human prion protein is reconstructed in the synthetic CC36 protein
Authors: Khrustalev Vladislav Victorovich , Khrustaleva Tatyana Aleksandrovna , Szpotkowski Kamil , Poboinev Victor Vitoldovich , Kakhanouskaya Katsiaryna Yurieuna 
Published: June 2016

ABSTRACTMechanisms of beta sheet formation by the human prion protein are not clear yet. In this work, we clarified the role of the region containing C‐half of the second helix and N‐half of the third helix of that protein in the process of alpha helix to beta sheet transition. Solid phase automatic synthesis of the original peptide (CC36: Cys179–Cys214) failed because of the beta hairpin formation in the region 206‐MERVVEQMC‐214 with a high beta strand potential. Using Met206Arg and Val210Arg substitutions, we increased the probability of alpha helix formation by that sequence. After that modification, the complete CC36 peptide with disulfide bond has been synthesized. Modified peptide has been studied by circular dichroism (CD) and fluorescence spectrography. According to the CD spectra analysis, the CC36 peptide contains 37% of residues in beta sheet and just 15% in helix. Thermal analysis under the control of CD shows that the secondary structure content of the peptide is stable from 5°C to 80°C. Dissociation of oligomers of the CC36 peptide finishes at 37°C according to the fluorescence analysis. The CC36 peptide is able to bind Mn2+ cations, which causes small temperature‐associated structural shifts at concentrations of 2 – 10·10−6 M. Predicted beta hairpin of the CC36 peptide (two beta strands are: 184‐IKQHTVT‐190 and 197‐TETDVKM‐205) should be the part of a longer beta hairpin from the scrapie form of the prion protein (PrPSc). Analogs of the CC36 peptide may be considered as antigens for the future development of a vaccine against PrPSc. Proteins 2016; 84:1462–1479. © 2016 Wiley Periodicals, Inc.
Accelerated molecular dynamics simulations of the octopamine receptor using GPUs: discovery of an alternate agonist‐binding position
Authors: Kastner Kevin W. , Izaguirre Jesús A. 
Published: June 2016

ABSTRACTOctopamine receptors (OARs) perform key biological functions in invertebrates, making this class of G‐protein coupled receptors (GPCRs) worth considering for insecticide development. However, no crystal structures and very little research exists for OARs. Furthermore, GPCRs are large proteins, are suspended in a lipid bilayer, and are activated on the millisecond timescale, all of which make conventional molecular dynamics (MD) simulations infeasible, even if run on large supercomputers. However, accelerated Molecular Dynamics (aMD) simulations can reduce this timescale to even hundreds of nanoseconds, while running the simulations on graphics processing units (GPUs) would enable even small clusters of GPUs to have processing power equivalent to hundreds of CPUs. Our results show that aMD simulations run on GPUs can successfully obtain the active and inactive state conformations of a GPCR on this reduced timescale. Furthermore, we discovered a potential alternate active‐state agonist‐binding position in the octopamine receptor which has yet to be observed and may be a novel GPCR agonist‐binding position. These results demonstrate that a complex biological system with an activation process on the millisecond timescale can be successfully simulated on the nanosecond timescale using a simple computing system consisting of a small number of GPUs. Proteins 2016; 84:1480–1489. © 2016 Wiley Periodicals, Inc.
Windswept hip deformity in children with cerebral palsy: A population-based prospective follow-up
Authors: Hägglund Gunnar , Lauge-Pedersen Henrik , Persson Bunke Måns , Rodby-Bousquet Elisabet 
Published: June 2016

Purpose To analyze the development of windswept hip deformity (WS) in a total population of children with cerebral palsy (CP) up to 20 years of age, the association between WS and hip dislocation, and femoral varus osteotomy and scoliosis, and the impact of a hip surveillance program on the subsequent incidence of WS. Methods This is a prospective study on children with CP in southern Sweden included in the Swedish follow-up programme and registry for CP (CPUP). All children born between 1990 and 1995 with CP were included; those born between 1990 and 1991 did not partake in the hip surveillance program until they were older (3–5 years of age) and served as a historic control group. Children born between 1992 and 1995 were included in the hip surveillance program from about 2 years of age and constituted the study group. Results In the control group, 12 of 68 children (18 %) developed WS. In the study group of 139 children, 13 (9 %) developed WS (p = 0.071). Of all 25 children with WS, 21 also developed scoliosis and 5 developed a hip dislocation. The number of children with WS starting in the lower extremities was significantly lower in the study group ( p = 0.028). No difference between the two groups was seen regarding WS that started in combination with scoliosis. Conclusion With early inclusion in a hip surveillance program and early treatment of contractures, it appears possible to reduce the frequency of WS starting in the lower extremities.
Distribution systems in omni-channel retailing
Authors: Hübner Alexander , Holzapfel Andreas , Kuhn Heinrich 
Published: June 2016

AbstractThe growing importance of online sales means that traditional bricks-and-mortar retailers need to create new distribution systems to serve customers through multiple channels. Building an effective and efficient omni-channel (OC) distribution system, however, leads to multiple challenges. The questions arise for example, from where online orders should be fulfilled, how delivery and return processes can be organized, and which context-specific OC distribution systems exist. Answering these questions retail research and practice require an overall view of the distribution concepts for direct-to-customer and store deliveries in OC retailing, including the associated return processes. This overall picture is still missing in the literature. We conducted an exploratory study to close this observable gap in the literature. This exploratory study is based on semi-structured interviews with major OC retailers in German-speaking countries and was complemented by market data research and discussions with further experts in the field of OC retailing. Based on the results of the study, the forward distribution system in OC retailing is characterized by the sources (supplier DCs, retailer DCs, stores) and destinations (home, store) which describe the options for store delivery, home delivery, and store pickup. Return processes are likewise characterized by the sources (store, home) and destinations (store, DC, return center). This framework forms the foundation for analyzing contextual criteria, identifying when the different conceptual designs are applied, determining industry-specific characteristics, and illustrating ways to further advance OC retailing. The present paper, therefore, contributes to the literature in three main areas: (1) it identifies and systematizes the forward and backward concepts in OC retailing, (2) it reveals application and development areas for achieving excellence in OC fulfillment and logistics, and (3) shows the need for developing sector- and context-specific OC distribution systems.