Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1
Authors: Garcia Edwin , Hayden Annette , Birts Charles , Britton Edward , Cowie Andrew , Pickard Karen , Mellone Massimiliano , Choh Clarisa , Derouet Mathieu , Duriez Patrick , Noble Fergus , White Michael J. , Primrose John N. , Strefford Jonathan C. , Rose-Zerilli Matthew , Thomas Gareth J. , Ang Yeng , Sharrocks Andrew D. , Fitzgerald Rebecca C. , Underwood Timothy J. ,   OCCAMS consortium, MacRae Shona , Grehan Nicola , Abdullahi Zarah , de la Rue Rachel , Noorani Ayesha , Elliott Rachael Fels , de Silva Nadeera , Bornschein Jan , O’Donovan Maria , Contino Gianmarco , Yang Tsun-Po , Chettouh Hamza , Crawte Jason , Nutzinger Barbara , Edwards Paul A. W. , Smith Laura , Miremadi Ahmad , Malhotra Shalini , Cluroe Alison , Hardwick Richard , Davies Jim , Ford Hugo , Gilligan David , Safranek Peter , Hindmarsh Andy , Sujendran Vijayendran , Carroll Nick , Turkington Richard , Hayes Stephen J. , Ang Yeng , Preston Shaun R. , Oakes Sarah , Bagwan Izhar , Save Vicki , Skipworth Richard J. E. , Hupp Ted R. , O’Neill J. Robert , Tucker Olga , Taniere Philippe , Owsley Jack , Crichton Charles , Schusterreiter Christian , Barr Hugh , Shepherd Neil , Old Oliver , Lagergren Jesper , Gossage James , Davies Andrew , Chang Fuju , Zylstra Janine , Sanders Grant , Berrisford Richard , Harden Catherine , Bunting David , Lewis Mike , Cheong Ed , Kumar Bhaskar , Parsons Simon L. , Soomro Irshad , Kaye Philip , Saunders John , Lovat Laurence , Haidry Rehan , Eneh Victor , Igali Laszlo , Welch Ian , Scott Michael , Sothi Shamila , Suortamo Sari , Lishman Suzy , Beardsmore Duncan , Anderson Charlotte , Smith Mike L. , Secrier Maria , Eldridge Matthew D. , Bower Lawrence , Achilleos Achilleas , Lynch Andy G. , Tavare Simon 
Published: September 2016

AbstractNew biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.