bluuebunny/crossref_metadata_2025_split huggingface dataset:
CREATION OF A GENETIC CONSTRUCTION FOR EFFECTIVE EXPRESSION OF α-HARPININ Sm-AMP-X GENE FROM STELLARIA MEDIA L. IN TOMATO PLANTS
Authors: Михель И.М. 
Published: September 2024

Томат является ценной овощной культурой, важным сырьем для пищевой промышленности и популярным салатным овощем. В 2019 г. мировой урожай томата составил более 180 млн т по данным FAO. Являясь важной культурой, томат уязвим для широкого спектра вирусных, грибных и бактериальных патогенов вследствие снижения генетического разнообразия культуры в процессе доместикации. Для томата описаны более 200 заболеваний по всему миру [1], что делает актуальной задачу защиты томата от фитопатогенов, в том числе, с применением генно-инженерных стратегий. Среди естественных стратегий, применяемых растениями для защиты от биологических патогенов, одной из наиболее распространенных является биосинтез веществ с антимикробной активностью.The tomato is a valuable vegetable crop, an important raw material for the food industry and a popular salad vegetable. In 2019, the world tomato crop amounted to more than 180 million tons according to the FAO. As an important crop, the tomato is vulnerable to a wide range of viral, fungal and bacterial pathogens due to the reduction in crop genetic diversity during domestication. More than 200 diseases have been described for tomato worldwide [1], which makes the task of protecting tomato from phytopathogens relevant, including the use of genetic engineering strategies. Among the natural strategies used by plants to protect against biological pathogens, one of the most common is the biosynthesis of substances with antimicrobial activity.
ARTEMISININ BIOSYNTHESIS GENES FOR TRANSFORMATION OF UNRELATED WORMWOOD PLANTS
Authors: Рахманов Б.К. , Имамходжаева А.С. , Усманов Д.Э. , Мирзахмедов М.Х. , Убайдуллаева Х.А. , Шерматов Ш.Э. , Буриев З.Т. , Абдурахмонов И.Ю. 
Published: September 2024

Артемизинин представляет собой сесквитерпеновый лактон, а полынь однолетняя (Artemisia annua L.) является его единственным источником. Артемизинин обладает терапевтическими свойствами против малярии, ревматоидного артрита, системной красной волчанки, аллергического контактного дерматита, рака и других заболеваний. В научных исследованиях коронавирусной инфекции COVID-19, ставшей источником всемирной пандемии, также был выявлен положительный потенциал артемизинина [1, 2, 3, 4]. В медицине артемизинин используется широко и учитывая тот факт, что его невозможно получить из подост в достаточно большом количестве, ожидается, что спрос на артемизинин значительно возрастет в ближайшем будущем.
Artemisinin is a sesquiterpene lactone, and mugwort (Artemisia annua L.) is its only source. Artemisinin has therapeutic properties against malaria, rheumatoid arthritis, systemic lupus erythematosus, allergic contact dermatitis, cancer and other diseases. In scientific studies of the coronavirus infection COVID-19, which became the source of the worldwide pandemic, the positive potential of artemisinin was also revealed [1, 2, 3, 4]. In medicine, artemisinin is widely used and given the fact that it cannot be obtained from the podost in sufficient quantities, the demand for artemisinin is expected to increase significantly in the near future.
Promising Microsatellite Loci of the Sugarbeet (BETA VULGARIS) Genome for Genetic Analysis of Lines and Hybrids
Authors: Шалаева Т.В. , Шилов И.А. 
Published: September 2024

Сахарная свёкла является важной технической культурой, на долю которой приходится примерно 40% мирового производства сахара. В настоящее время к числу важнейших хозяйственно-ценных требований к сорту, помимо выхода сахара с единицы сырья и площади посева, относятся повышение технологической пригодности сырья, получение семян с высокими посевными и физическими свойствами, толерантность к гербицидам, устойчивость к болезням, вредителям, факторам внешней среды, а главное – рентабельность производства в области семеноводства и возделывания коммерческих посевов сахарной свёклы.
Sugar beet is an important industrial crop, accounting for approximately 40% of the world's sugar production. At present, the most important economically valuable requirements for a variety, in addition to the yield of sugar per unit of raw material and planting area, include increasing the technological suitability of raw materials, obtaining seeds with high sowing and physical properties, tolerance to herbicides, resistance to diseases, pests, and environmental factors. environment, and most importantly - the profitability of production in the field of seed production and cultivation of commercial sugar beet crops.
APPLICATION OF THE METHOD OF ELECTROPHORETIC SEPARATION OF GLUTEN STORAGE PROTEINS FOR ASSESSMENT OF THE GENETIC STRUCTURE OF THE POPULATION OF COMMON WHEAT (T. AESTIVUM) BY GLU LOCUS
Authors: Стрембовский И.В. , Дивашук М.Г. 
Published: September 2024

Традиционным прямым, и как следствие более точным, методом оценки качественных показателей клейковины у хлебопекарных сортов пшеницы является пробная лабораторная выпечка [1]. Как и многие прямые методы оценки данный метод сопряжён с уничтожением большого объёма семян, а значит его проведение возможно только на поздних этапах селекционного процесса, когда какие-либо мероприятия по улучшению сортообразца провести уже невозможно. Альтернативой прямым выступают косвенные методы оценки (при оценке клейковины - метод седиментации, определение ИДК и тд [2]), которые не требуют большого расхода семян на проведение, но и существенно уступают в точности прямым методам [3].
The traditional direct, and as a result more accurate, method for assessing the quality indicators of gluten in baking wheat varieties is trial laboratory baking [1]. Like many direct assessment methods, this method is associated with the destruction of a large volume of seeds, which means that it can only be carried out at the later stages of the breeding process, when it is no longer possible to carry out any measures to improve the variety sample. An alternative to direct methods are indirect methods of assessment (in the assessment of gluten - the sedimentation method, the determination of IDK, etc. [2]), which do not require a large consumption of seeds for carrying out, but are significantly inferior in accuracy to direct methods [3].
CELL SELECTION OF WINTER GARLIC FOR RESISTANCE TO INCREASED SOIL ACIDITY
Authors: Азопкова М.А. 
Published: September 2024

Чеснок (Allium sativum L.) является ценной овощной и лекарственной культурой. Это вегетативно размножаемое растение требовательно к плодородию почвы и уровню рН 6,5-7 [1]. В настоящее время общая площадь кислых почв в России достигла 50 млн га. По результатам агрохимического обследования на 2016 г. кислые почвы (рН ≤5,5) занимали более 34,7 млн. га пашни (33%) [2,3]. При снижении рН ниже 5,5 урожайность сельскохозяйственных культур снижается до 30 %. При использовании современных биотехнологических методов можно получить в культуре in vitro генотипы, являющиеся ценным исходным материалом для селекции.
Garlic (Allium sativum L.) is a valuable vegetable and medicinal crop. This vegetatively propagated plant is demanding on soil fertility and pH level of 6.5-7 [1]. At present, the total area of acidic soils in Russia has reached 50 million hectares. According to the results of an agrochemical survey for 2016, acidic soils (pH ≤5.5) occupied more than 34.7 million hectares of arable land (33%) [2,3]. With a decrease in pH below 5.5, crop yields are reduced to 30%. With the use of modern biotechnological methods, it is possible to obtain genotypes in culture in vitro, which are a valuable source material for selection.
INTRODUCTION TO THE IN VITRO CULTURE OF BAIKAL SCULPTURE
Authors: Броноских Е.Д. , Пивоварова Н.С. 
Published: September 2024

Шлемник байкальский – многолетнее растение семейства Яснотковых, которое содержит широкий спектр биологически активных веществ: флавоноиды, кумарины, пирокатехины и другие. Ареал распространения растения в России ограничен [2]. Учитывая это, его заготовка приводит к истощению природных популяций, поэтому актуальным становится разработка протоколов микроклонального размножения. Для асептического введения в культуру in vitro использовали семена Шлемника байкальского, которые проращивали на питательной среде Мурасиге — Скуга с добавлением НУК 6 мг/л, кинетина 2 мг/л, тиамина 1 мг/л. Проращивание проводили в темноте при температуре +25°С и относительной влажности воздуха 60-70%.Baikal skullcap is a perennial plant of the Lamiaceae family, which contains a wide range of biologically active substances: flavonoids, coumarins, pyrocatechins and others. The distribution area of the plant in Russia is limited [2]. Given this, its harvesting leads to the depletion of natural populations, so the development of micropropagation protocols becomes relevant. For aseptic introduction into culture in vitro, we used the seeds of Baikal skullcap, which were germinated on a Murashige-Skoog nutrient medium with the addition of NAA 6 mg/l, kinetin 2 mg/l, thiamine 1 mg/l. Germination was carried out in the dark at a temperature of +25°C and a relative humidity of 60-70%.
CYTOKININS AS POSSIBLE DETERMINANTS OF PROGRAMMED CELL DEATH OF POLLEN TUBES IN IN VITRO AND IN VIVO SYSTEMS (ON MODEL OBJECTS OF TOMATOES S. HABROCHAITES, S. PENNELLII, S. CHILENSE)
Authors: Ханина Т.П. , Голиванов Я.Ю. , Захарова Е.В. 
Published: September 2024

Половое размножение покрытосеменных – очень избирательный процесс. Материнские ткани пестика способны различать собственные и чужие пыльцевые зерна, самосовместимые и самонесовместимые. Эта избирательность сопровождается огромным разнообразием клеточных поверхностей мужских и женских половых органов, и их структур. Пестик хорошо организован не только для принятия пыльцевых зерен и роста пыльцевых трубок, но и имеет механизмы, которые блокируют их рост на разных стадиях. Одну из ведущих ролей в этих процессах играют фитогормоны. Ранее в нашей группе получены убедительные доказательства того, что цитокинины (ЦК) участвуют в развитии мужского гаметофита и в функционировании механизма гаметофитной самонесовместимости S-РНКазного типа у петунии (P. hybrida L.) (Zakharova et al., 2021).
Sexual reproduction in angiosperms is a highly selective process. The maternal tissues of the pistil are able to distinguish between self and foreign pollen grains, self-compatible and self-incompatible. This selectivity is accompanied by a huge variety of cell surfaces of male and female genital organs, and their structures. The pistil is well organized not only to accept pollen grains and grow pollen tubes, but also has mechanisms that block their growth at different stages. Phytohormones play one of the leading roles in these processes. Previously, our group obtained convincing evidence that cytokinins (CK) are involved in the development of the male gametophyte and in the functioning of the mechanism of gametophytic self-incompatibility of the S-RNase type in petunia (P. hybrida L.) (Zakharova et al., 2021).
DIGITAL PHENOTYPING IN THE FIELDS AND IN THE LABORATORY
Authors: Литвинов Д.Ю. 
Published: September 2024

Высокопроизводительное цифровое фенотипирование (ВЦФ) - новое быстро развивающееся направление фенотипирования, которое должно преодолеть отставание в производительности фенотипирования растений от производительности современных высокопроизводительных методов генотипирования. Для проведения ВЦП созданы и продолжают совершенствоваться платформы с масштабом областей фенотипирования от отдельного растения до промышленных сельскохозяйственных полей и значительной части сухопутной поверхности земли.
High-throughput digital phenotyping (HDP) is a new rapidly developing area of phenotyping, which should overcome the lag in plant phenotyping productivity from the productivity of modern high-throughput genotyping methods. Platforms with the scale of phenotyping areas from a single plant to industrial agricultural fields and a significant part of the land surface of the earth have been created and continue to be improved for the WDC.
CYTOLOGICAL EVALUATION IN BIOTECHNOLOGY OF BETA VULGARIS L.
Authors: Колесникова Е.О. , Пономарева С.В. , Донских Е.И. , Музалевская А.А. , Бердников Р.В. 
Published: September 2024

Сахарная свекла в Российской Федерации является основным источником получения одного из важнейших продуктов питания – сахара. В условиях интенсификации сельского хозяйства главной задачей селекции Beta vulgaris L. является создание отечественных высокопродуктивных гибридов. При этом существует необходимость использования биотехнологических приемов для получения новых форм либо выравнивания и клонирования компонентов имеющихся селекционных достижений. Для создания диплоидных гибридов в настоящее время используют F1-гибрид между инбредной МС линией и неродственным О-типом как материнский компонент и открыто пылящую линию в качестве отцовского компонента-опылителя.
Sugar beet in the Russian Federation is the main source of one of the most important food products - sugar. Under the conditions of agricultural intensification, the main task of breeding Beta vulgaris L. is the creation of domestic highly productive hybrids. At the same time, there is a need to use biotechnological methods to obtain new forms or to equalize and clone the components of existing breeding achievements. To create diploid hybrids, an F1 hybrid between an inbred MC line and an unrelated O-type is currently used as a maternal component and an open dusting line as a pollinating paternal component.
THE USE OF THE STUDY STICK AS AN INITIAL EXPLANT IN CLONAL MICROPROPAGATION OF STERILE GRAINS
Authors: Алкубеси М. , Блинков А.О. , Черноок А.Г. 
Published: September 2024

Отдалённая гибридизация, получение удвоенных гаплоидов и амфидиплоидов являются широко распространёнными методами создания исходного материала в селекции злаков. Однако, некоторый ценный генетический материал у отдалённых гибридов мягкой и твёрдой пшеницы, а также других злаков, может теряться из-за проблемы стерильности данных растений, вызванной наличием негомологичных субгеномов и трудностей полиплоидизации. При невозможности получения семян от самоопыления, использование клонального микроразмножения может помочь сохранить и размножить эти растения.
Distant hybridization, obtaining doubled haploids and amphidiploids are widely used methods for creating starting material in cereal breeding. However, some valuable genetic material in distant hybrids of soft and durum wheat, as well as other cereals, may be lost due to the problem of sterility of these plants caused by the presence of non-homologous subgenomes and difficulties in polyploidization. If it is not possible to obtain seeds from self-pollination, the use of clonal micropropagation can help to conserve and propagate these plants.
AI Based Smart Supervision System
Authors: Ms. Harshada Vijay Mandlik  , Ms. Vaishnavi Tukaram Dorik  
Published: September 2024

Abstract: The integrity and fairness of examinations are continually compromised by deceptive practices such as whispering, head movements, and unauthorized hand contacts. These unethical activities pose a serious threat to the credibility of examinations, necessitating the development of a robust model for real-time supervision and control. This research aims to introduce an innovative model designed to detect and prevent such unethical behaviour during examinations, there by upholding the principles of fairness and impartiality. The suggested monitoring system can be used in colleges, universities, and schools to identify and observe students engaging in suspicious activities. By implementing this monitoring system, we aim to stop and address cheating issues since it goes against ethical standards. The proposed invigilation model can be implemented in colleges, universities, and schools to detect and monitor student suspicious activities.
Hopefully, through the implementation of the proposed invigilation system, we can prevent and solve the problem of cheating because it is unethical. Each exam room needs a head invigilator to make sure the exams are honest and address any issues that may arise. That’s why we implement the AI Base Smart Supervision System.
Precursors and pathways: dynamically informed extreme event forecasting demonstrated on the historic Emilia-Romagna 2023 flood
Authors: Dorrington Joshua , Wenta Marta , Grazzini Federico , Magnusson Linus , Vitart Frederic , Grams Christian M. 
Published: September 2024

Abstract. The ever-increasing complexity and data volumes of numerical weather prediction demand innovations in the analysis and synthesis of operational forecast data. Here we show how dynamical thinking can offer directly applicable forecast information, taking as a case study the extreme northern Italy flooding of May 2023. We compare this event with long-lasting historical northern Italy rainfall events in order to determine (a) why it was so extreme, (b) how well it was predicted, and (c) how we may improve our predictions of such extremes. Lagrangian analysis shows, in line with previous work, that 48-hourly extreme rainfall in Italy can be caused by moist air masses originating from the North Atlantic; North Africa; and, to a lesser extent, eastern Europe, with compounding moisture contributions from all three regions driving the May 2023 event. We identify the large-scale precursors of typical northern Italy rainfall extremes based on geopotential height and integrated vapour transport fields. We show in European Centre for Medium-Range Weather Forecasts (ECMWF) operational forecasts that a precursor perspective was able to identify the growing possibility of the Emilia-Romagna extreme event 8 d beforehand – 4 d earlier than the direct precipitation forecast. Such dynamical precursors prove to be well suited for identifying and interpreting predictability barriers and could help build forecasters' understanding of unfolding extreme scenarios in the medium range. We conclude by discussing the broader implications and operational potential of dynamically rooted metrics for understanding and predicting extreme events, both in retrospect and in real time.
33. CHẤT LƯỢNG CUỘC SỐNG VÀ MỘT SỐ YẾU TỐ LIÊN QUAN Ở NGƯỜI BỆNH MẮC BỆNH PHỔI TẮC NGHẼN MẠN TÍNH TẠI BỆNH VIỆN ĐA KHOA XANH PÔN
Authors: Tân Phạm Văn , Huyền Nguyễn Thị Hoa , My Hạc Huyền , Thu Nguyễn Thị Hoàng , Ngọc Nguyễn Bích 
Published: September 2024

Mục tiêu: Mô tả chất lượng cuộc sống và xác định một số yếu tố liên quan tới chất lượng cuộc sống của người bệnh mắc bệnh phổi tắc nghẽn mạn tính.
Đối tượng và phương pháp: Nghiên cứu mô tả cắt ngang được thực hiện trên 121 người bệnh điều trị tại Khoa Nội tổng hợp 1, Bệnh viện Đa khoa Xanh Pôn từ tháng 8/2022 đến tháng 12/2022, sử dụng bộ công cụ CAT để đánh giá chất lượng cuộc sống người bệnh. Sau khi làm sạch, loại bỏ các phiếu không đạt yêu cầu, 90 phiếu được đưa vào phân tích.
Kết quả: Nghiên cứu cho thấy, điểm trung bình chất lượng cuộc sống chung là 22,22 ± 4,49 điểm, 54,4% người bệnh có chất lượng cuộc sống bị ảnh hưởng nặng bởi bệnh phổi tắc nghẽn mạn tính. Có sự khác biệt có ý nghĩa thống kê giữa điểm trung bình chất lượng cuộc sống theo thang điểm CAT với thể trạng theo BMI và thời gian mắc bệnh (p < 0,05).
Kết luận: Chất lượng cuộc sống của người bệnh mắc bệnh phổi tắc nghẽn mạn tính bị ảnh hưởng rõ rệt từ mức trung bình đến rất nặng. Hoạt động chăm sóc điều trị cần chú ý đến người bệnh có BMI < 18,5 kg/m2 và/hoặc thời gian mắc bệnh trên 5 năm nhằm hỗ trợ người bệnh tăng cường sức khỏe tổng thể và nâng cao chất lượng cuộc sống.
O PAPEL DO COORDENADOR PEDAGÓGICO NA FORMAÇÃO EM SERVIÇO
Authors: De Souza Maria Wanilsa Silva , Fernandes Leonete Cunha , Carneiro Daniel Santos dos , Costa Aline Cristina Nascimento da Silva 
Published: September 2024

Este trabalho, que aborda o papel do coordenador pedagógico em uma das suas variadas atribuições que é a formação em serviço, foi apresentado como trabalho final do curso de especialização em Gestão, Coordenação e Supervisão escolar. Tendo como um de seus objetivos discutir os desafios e estratégias enfrentados por esse profissional no seu papel de formador em serviço nas unidades escolares da rede municipal de ensino de Fortaleza. Para isso foi realizada uma pesquisa bibliográfica, exploratória e documental, considerando a escassez de referências sobre a temática ora pretendida. Foi então escolhido como contexto para fundamentar a discussão, a formação continuada oferecida pela rede municipal de educação de Fortaleza aos seus docentes. A partir dos resultados da pesquisa foi possível constatar a importância e o valor do coordenador pedagógico nas unidades escolares para a implementação das políticas públicas bem como no processo contínuo de busca por uma qualidade na educação oferecida nas instituições públicas da educação básica.
Combustion instabilities in single and multi-flame systems
Authors: Worth Nicholas 
Published: September 2024

Gas turbine engines are an integral part of the global energy production and infrastructure. The ability to rapidly scale their power output makes them an attractive partner to intermittent renewable energy sources, meaning they are likely to retain their importance throughout the energy transition. They are also widely used for propulsion, with their high power to weight ratio, and their fuels’ energy to weight ratio making them difficult to replace for long distance transportation. There has long been a focus to reduce the emission of pollutants such as nitrous oxides (NOx) from gas turbines, but the impending climate crisis means there is a growing need to also eliminate the emission of carbon dioxide (CO2). One promising method of achieving this is to switch from hydrocarbon-based fuels such as natural gas (mainly composed of methane – CH4), to fuel blends composed of increasing proportions of carbon-free fuels such as hydrogen (H2) and ammonia (NH3). However, switching to these fuels is challenging due to substantially different fuel properties. Designing future gas turbine systems that can operate in a fuel-flexible manner, accepting a wide variety of alternative carbon-free fuels is not straightforward, and hindered by our incomplete understanding of these fuels and some important phenomena which occur during their use, including flame stabilization, combustion instabilities, and harmful emissions. In this talk recent experimental work on thermoacoustic instabilities in single flames and annular combustors will be presented, with an emphasis on the careful excitation of modes of interest, and the effects of realistic boundary conditions. Work will also be presented from a simple axially staged combustor, in which the concept of flexible fueling is briefly explored.