bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microwear–mesowear congruence and mortality bias in rhinoceros mass-death assemblages
Authors: Mihlbachler Matthew C. , Campbell Daniel , Chen Charlotte , Ayoub Michael , Kaur Pawandeep 
Published: November 2017

AbstractAlthough we do not know the cause of death of most fossil animals, mortality is often associated with ecological stress due to seasonality and other stochastic events (droughts, storms, volcanism) that may have caused shifts in feeding ecology preceding death. In these instances, dental microwear, which reflects feeding ecology in a narrow window of time, may provide a biased view of diet. Mesowear, another dental-wear proxy based on the morphology of worn cusps, requires macroscopic amounts of dental wear and reflects diet for a longer interval and may be less prone to bias from near-death ecological stress. We compared congruence between microwear and mesowear of North American, fossil rhinocerotid mass-death assemblages and collections of hunted modern rhinocerotids to test the hypothesis that fossil assemblages yield more incongruous microwear and mesowear data as a result of near-death ecological disturbances. In extant rhinos, both mesowear and microwear are associated with diet and height of the feeding environment. Mesowear and microwear in the modern rhinocerotid collections are statistically correlated, with strong relationships between average mesowear scores and labially distributed dental microwear. In contrast, a relationship between mesowear and microwear was not observed among the fossil rhinocerotid assemblages. Mesowear suggests that the fossil rhinos had low-abrasion diets, suggesting that they fed from clean, possibly tall vegetation. Some, but not all, mass-death assemblages produce microwear data with excessive scratches and/or pits compared with expectations based on mesowear results, suggesting that dental microwear was altered shortly before death in some but not all of the fossil assemblages. The dental-wear proxies available to paleoecologists provide a mosaic of dietary evidence reflecting diet over long (mesowear) and more abbreviated (microwear) periods of time that, together, provide a richer understanding of feeding ecology and its relationship to environment, seasonal change, and other ecological disturbances.
POLITICAL BOUNDARY VERSUS SOCIAL CONTEXT: DYNAMICS OF SOCIOECONOMIC DIFFERENTIALS IN FERTILITY IN INDIAN STATES
Authors: Arunachalam Dharma , Navaneetham Kannan , Forrest Walter 
Published: November 2017

SummaryUsing data from India’s first (1992–93) and third (2005–06) National Family Health Surveys (NFHS-I and NFHS-III) this study examined the fertility differentials between major social groups and the extent to which these varied between states and over time. The analysis was based on a sample of 54,030 and 55,369 currently married women aged 15–34 in the NFHS-I and NFHS-III respectively. Reported parity and desired family size were used to assess variations in fertility behaviour. The results show that interstate variation in childbearing patterns within social groups was at least as high as, if not higher than, variation between states (net of other influences) in both periods, 1992–93 and 2005–06. The variations among Hindus, the poor and Muslims were more noticeable than for other groups. These variations did not decline between 1992–93 and 2005–06 and may have even increased slightly for some groups. Further, there was no consistent north–south divide in either fertility behaviour or desired family size. Together, these results may point to the gradual disappearance of the influences that were once unique to southern or northern India, and the simultaneous emergence of social, political, economic and cultural forces that are pan-Indian in their reach.
Effects of a parent training programme for the treatment of young children with separation anxiety disorder
Authors: Mayer-Brien Sandra , Turgeon Lyse , Lanovaz Marc J. 
Published: November 2017

AbstractCognitive behavioural therapy (CBT) has been shown to be an effective treatment for anxiety disorders in children. However, the majority of studies conducted to date have included heterogeneous samples of children combining social anxiety disorder, generalized anxiety disorder and separation anxiety disorder (SAD) together. Few studies have examined the efficacy of CBT to specifically treat SAD. Moreover, research on the impact of CBT for SAD in children younger than 7 years old has been very limited. To address these issues, we examined the effects of a CBT parent training programme with six children with SAD aged between 4 and 7 years using a non-concurrent multiple baseline design. Parents completed semi-structured interviews, questionnaires and daily diaries to assess their child's anxiety symptoms, parental practices and parental stress. Results indicated that five of six children no longer met the criteria for SAD diagnosis after treatment and at 3-month follow-up. Moreover, the daily diaries showed that four of six children presented a systematic favourable change of the total weekly frequency of SAD symptoms. Finally, four mothers reported a decrease in overprotection, but we observed no changes in parental stress.
Dihydroartemisinin selectively inhibits PDGFRα-positive ovarian cancer growth and metastasis through inducing degradation of PDGFRα protein
Authors: Li Xiaoguang , Ba Qian , Liu Yanling , Yue Qingxi , Chen Peizhan , Li Jingquan , Zhang Haibing , Ying Hao , Ding Qiurong , Song Haiyun , Liu Hong , Zhang Ruiwen , Wang Hui 
Published: November 2017

AbstractTo develop traditional medicines as modern pharmacotherapies, understanding their molecular mechanisms of action can be very helpful. We have recently reported that Artemisinin and its derivatives, which are clinically used anti-malarial drugs, have significant effects against ovarian cancer, but the direct molecular targets and related combination therapy have been unclear. Herein, we report that dihydroartemisinin, one of the most active derivatives of Artemisinin, directly targets platelet-derived growth factor receptor-alpha (PDGFRα) to inhibit ovarian cancer cell growth and metastasis. Dihydroartemisinin directly binds to the intercellular domain of PDGFRα, reducing its protein stability by accelerating its ubiquitin-mediated degradation, which further inactivates downstream phosphoinositide 3-Kinase and mitogen-activated protein kinase pathways and subsequently represses epithelial–mesenchymal transition, inhibiting cell growth and metastasis of PDGFRα-positive ovarian cancer in vitro and in vivo. A combinational treatment reveals that dihydroartemisinin sensitizes ovarian cancer cells to PDGFR inhibitors. Our clinical study also finds that PDGFRα is overexpressed and positively correlated with high grade and metastasis in human ovarian cancer. Considering that Artemisinin compounds are currently clinically used drugs with favorable safety profiles, the results from this study will potentiate their use in combination with clinically used PDGFRα inhibitors, leading to maximal therapeutic efficacy with minimal adverse effects in PDGFRα-positive cancer patients. These findings also shed high light on future development of novel Artemisinin-based targeted therapy.
New design model for high efficiency cylindrical diffractive microlenses
Authors: Li Ye , Zhao Huan , Feng Sheng-Fei , Ye Jia-Sheng , Wang Xin-Ke , Sun Wen-Feng , Han Peng , Zhang Yan 
Published: November 2017

AbstractA new model, i.e., the decreasing thickness model (DTM) is proposed and employed for designing the cylindrical diffractive microlenses (CDMs). Focal performances of the designed CDMs are theoretically investigated by solving Maxwell’s equations with the boundary element method. For comparison, the CDMs designed by the traditional equal thickness model (ETM) are also studied. Theoretical simulations demonstrate that focal performances of the designed CDMs are improved a lot via replacing the traditional ETM with the proposed DTM. Concretely, the focal efficiency is heightened and the focal spot size is shrunk. Experimental measurements verify the theoretical simulations well. Especially, the above-mentioned improvements become more prominent for the CDM with a higher numerical aperture.
Calf Circumference as a Novel Tool for Risk of Disability of the Elderly Population
Authors: Sun Yu-Shan , Kao Tung-Wei , Chang Yaw-Wen , Fang Wen-Hui , Wang Chung-Ching , Wu Li-Wei , Yang Hui-Fang , Liaw Fang-Yih , Chen Wei-Liang 
Published: November 2017

AbstractDisability became increasingly common with age, and crude rates of disability were rising around the globe. The aim of this study was to investigate the association between calf circumference (CC) and disability in the U.S. elderly population. From the 1999–2006 National Health and Nutrition Examination Survey, a total of 4,245 participants with an age range of 60–84 years were included. Disability was defined as the total number of difficulties within the following 5 major domains of disability, such as activities of daily living (ADL), instrumental ADL, general physical activities, lower extremity mobility, and leisure and social activities. The association between CC and disability was investigated through the regression model adjusted for multiple covariates. According to the fully adjusted model regarding disability, the β coefficients for each quartile of increasing CC were −0.041 for quartile 2 (P = 0.096), −0.060 for quartile 3 (P = 0.027), and −0.073 for quartile 4 (P = 0.026) respectively, compared with lowest quartile. There was a negative association between CC and disability among the elderly population. Calf circumference may be a novel risk assessment for disability of elderly people.
Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands
Authors: Fraioli Roberta , Tsimbouri Penelope M. , Fisher Leanne E. , Nobbs Angela H. , Su Bo , Neubauer Stefanie , Rechenmacher Florian , Kessler Horst , Ginebra Maria-Pau , Dalby Matthew J. , Manero José M. , Mas-Moruno Carlos 
Published: November 2017

AbstractEngineering the interface between biomaterials and tissues is important to increase implant lifetime and avoid failures and revision surgeries. Permanent devices should enhance attachment and differentiation of stem cells, responsible for injured tissue repair, and simultaneously discourage bacterial colonization; this represents a major challenge. To take first steps towards such a multifunctional surface we propose merging topographical and biochemical cues on the surface of a clinically relevant material such as titanium. In detail, our strategy combines antibacterial nanotopographical features with integrin selective synthetic ligands that can rescue the adhesive capacity of the surfaces and instruct mesenchymal stem cell (MSC) response. To this end, a smooth substrate and two different high aspect ratio topographies have been produced and coated either with an αvβ3-selective peptidomimetic, an α5β1-selective peptidomimetic, or an RGD/PHSRN peptidic molecule. Results showed that antibacterial effects of the substrates could be maintained when tested on pathogenic Pseudomonas aeruginosa. Further, functionalization increased MSC adhesion to the surfaces and the αvβ3-selective peptidomimetic-coated nanotopographies promoted osteogenesis. Such a dual physicochemical approach to achieve multifunctional surfaces represents a first step in the design of novel cell-instructive biomaterial surfaces.
Identification of novel genetic loci GAL3ST4 and CHGB involved in susceptibility to leprosy
Authors: Yuan Youhua , You Yuangang , Wen Yan , Liu Jian , Li Huanying , Zhang Yumeng , Wu Nan , Liu Shuang , Zhang Shanshan , Chen Jiazhen , Ai Jingwen , Zhang Wenhong , Zhang Ying 
Published: November 2017

AbstractLeprosy has long been thought to have a strong genetic component, and so far, only positional cloning and genomewide association studies have been used to study the genetic susceptibility to leprosy,while whole exome sequencing (WES) approach has not yet been applied. In this study, we used WES approach on four leprosy patients and four healthy control relatives from two leprosy families. We found three new susceptible loci of leprosy, one in GAL3ST4 and two in CHGB. We went on to validate the findings of WES using 151 leprosy cases and 226 healthy controls by Sanger sequencing. Stratified by gender, GAL3ST4 was found to be the susceptible gene only for the female population, and CHGB48 and CHGB23 were susceptibile to leprosy for the male population, respectively). Moreover, the gene expression levels of the three susceptible loci were measured by real-time PCR after the stimulation by M. leprae antigens in the PBMC (peripheral blood mononuclear cells) of 69 healthy people. The results showed that the female subjects with high frequent genotype in GAL3ST4 had a fivefold elevated expression. We suggest the polymorphisms in GAL3ST4 in different population are associated with increased risk of leprosy.
Ellipsoidal mirror for two-dimensional 100-nm focusing in hard X-ray region
Authors: Yumoto Hirokatsu , Koyama Takahisa , Matsuyama Satoshi , Kohmura Yoshiki , Yamauchi Kazuto , Ishikawa Tetsuya , Ohashi Haruhiko 
Published: November 2017

AbstractCutting-edge hard X-ray microscopy strongly depends on sophisticated focusing optics and ultrabright X-ray sources at synchrotron-radiation and X-ray free-electron laser (XFEL) facilities. These facilities typically provide two-dimensional nanofocusing X-ray beams by combining one-dimensional focusing mirrors. However, single-reflecting two-dimensional focusing mirrors with an ellipsoidal surface, which are well-known to possess high efficiency, have limited microfocusing applications. In this paper, we present an ultrahigh-precision ellipsoidal mirror for two-dimensional X-ray nanofocusing by overcoming the difficulties faced in the manufacturing process of its aspherical surface, including the surface-processing methods and surface metrology. The developed mirror has nanoscale accuracy, and it achieves focus size of 85 nm × 125 nm (full width at half maximum) using 7-keV X-rays. Two-dimensional focus was demonstrated in the same focal plane by resolving 50-nm test structures by scanning X-ray microscopy using a focusing beam. These achievements represent an important first step toward realizing two-dimensional aspherical mirrors with complex designs, in addition to ultralow loss and unprecedented small focusing property for extensive optical applications in synchrotron-radiation and XFEL facilities as well as in other scientific fields that require ultraprecision optical surfaces.
Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins
Authors: Sethupathy Sivasamy , Ananthi Sivagnanam , Selvaraj Anthonymuthu , Shanmuganathan Balakrishnan , Vigneshwari Loganathan , Balamurugan Krishnaswamy , Mahalingam Sundarasamy , Pandian Shunmugiah Karutha 
Published: November 2017

AbstractSerratia marcescensis one of the important nosocomial pathogens which rely on quorum sensing (QS) to regulate the production of biofilm and several virulence factors. Hence, blocking of QS has become a promising approach to quench the virulence ofS. marcescens. For the first time, QS inhibitory (QSI) and antibiofilm potential ofActinidia deliciosahave been explored againstS. marcescensclinical isolate (CI).A. deliciosapulp extract significantly inhibited the virulence and biofilm production without any deleterious effect on the growth. Vanillic acid was identified as an active lead responsible for the QSI activity. Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode inS. marcescensCI, ATCC 14756 and MG1. Furthermore vanillic acid increased the survival ofCaenorhabditis elegansuponS. marcescensinfection. Proteomic analysis and mass spectrometric identification of differentially expressed proteins revealed the ability of vanillic acid to modulate the expression of proteins involved in S-layers, histidine, flagellin and fatty acid production. QSI potential of the vanillic acid observed in the current study paves the way for exploring it as a potential therapeutic candidate to treatS. marcescensinfections.
Developing a Machine Learning System for Identification of Severe Hand, Foot, and Mouth Disease from Electronic Medical Record Data
Authors: Liu Guangjian , Xu Yi , Wang Xinming , Zhuang Xutian , Liang Huiying , Xi Yun , Lin Fangqin , Pan Liyan , Zeng Taishan , Li Huixian , Cao Xiaojun , Zhao Gansen , Xia Huimin 
Published: November 2017

AbstractChildren of severe hand, foot, and mouth disease (HFMD) often present with same clinical features as those of mild HFMD during the early stage, yet later deteriorate rapidly with a fulminant disease course. Our goal was to: (1) develop a machine learning system to automatically identify cases with high risk of severe HFMD at the time of admission; (2) compare the effectiveness of the new system with the existing risk scoring system. Data on 2,532 HFMD children admitted between March 2012 and July 2015, were collected retrospectively from a medical center in China. By applying a holdout strategy and a 10-fold cross validation method, we developed four models with the random forest algorithm using different variable sets. The prediction system HFMD-RF based on the model of 16 variables from both the structured and unstructured data, achieved 0.824 sensitivity, 0.931 specificity, 0.916 accuracy, and 0.916 area under the curve in the independent test set. Most remarkably, HFMD-RF offers significant gains with respect to the commonly used pediatric critical illness score in clinical practice. As all the selected risk factors can be easily obtained, HFMD-RF might prove to be useful for reductions in mortality and complications of severe HFMD.
Twitter-derived neighborhood characteristics associated with obesity and diabetes
Authors: Nguyen Quynh C. , Brunisholz Kimberly D. , Yu Weijun , McCullough Matt , Hanson Heidi A. , Litchman Michelle L. , Li Feifei , Wan Yuan , VanDerslice James A. , Wen Ming , Smith Ken R. 
Published: November 2017

AbstractNeighborhood characteristics are increasingly connected with health outcomes. Social processes affect health through the maintenance of social norms, stimulation of new interests, and dispersal of knowledge. We created zip code level indicators of happiness, food, and physical activity culture from geolocated Twitter data to examine the relationship between these neighborhood characteristics and obesity and diabetes diagnoses (Type 1 and Type 2). We collected 422,094 tweets sent from Utah between April 2015 and March 2016. We leveraged administrative and clinical records on 1.86 million individuals aged 20 years and older in Utah in 2015. Individuals living in zip codes with the greatest percentage of happy and physically-active tweets had lower obesity prevalence—accounting for individual age, sex, nonwhite race, Hispanic ethnicity, education, and marital status, as well as zip code population characteristics. More happy tweets and lower caloric density of food tweets in a zip code were associated with lower individual prevalence of diabetes. Results were robust in sibling random effects models that account for family background characteristics shared between siblings. Findings suggest the possible influence of sociocultural factors on individual health. The study demonstrates the utility and cost-effectiveness of utilizing existing big data sources to conduct population health studies.
Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
Authors: Mamer Spencer B. , Chen Si , Weddell Jared C. , Palasz Alexandra , Wittenkeller Ashley , Kumar Manu , Imoukhuede P. I. 
Published: November 2017

AbstractNearly all studies of angiogenesis have focused on uni-family ligand-receptor binding, e.g., VEGFs bind to VEGF receptors, PDGFs bind to PDGF receptors, etc. The discovery of VEGF-PDGFRs binding challenges this paradigm and calls for investigation of other ligand-receptor binding possibilities. We utilized surface plasmon resonance to identify and measure PDGF-to-VEGFR binding rates, establishing cut-offs for binding and non-binding interactions. We quantified the kinetics of the recent VEGF-A:PDGFRβ interaction for the first time with KD = 340 pM. We discovered new PDGF:VEGFR2 interactions with PDGF-AA:R2 KD = 530 nM, PDGF-AB:R2 KD = 110 pM, PDGF-BB:R2 KD = 40 nM, and PDGF-CC:R2 KD = 70 pM. We computationally predict that cross-family PDGF binding could contribute up to 96% of VEGFR2 ligation in healthy conditions and in cancer. Together the identification, quantification, and simulation of these novel cross-family interactions posits new mechanisms for understanding anti-angiogenic drug resistance and presents an expanded role of growth factor signaling with significance in health and disease.
Involvement of cecropin B in the formation of the Aedes aegypti mosquito cuticle
Authors: Liu Wei-Ting , Tu Wu-Chun , Lin Chao-Hsiung , Yang Ueng-Cheng , Chen Cheng-Chen 
Published: November 2017

AbstractIn this study, we found a mosquito antimicrobial peptide (AMP), Aedes aegypti cecropin B (Aacec B), was expressed constitutively in pupae. Knockdown in the pupae of Aacec B using double-stranded RNA (dsRNA) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. Simultaneous injection of Aacec B dsRNA and Aacec B peptide into pupae significantly reduced this mortality and no deformed adults then emerged. The expression levels of Ae. aegypti prophenoloxidase (AaPPO) 3 and AaPPO 4 were significantly reduced in the Aacec B knockdown pupae. Exogenous Aacec B peptide significantly enhanced the transcription of AaPPO 3 in pupae. Knockdown of AaPPO 3 in pupae caused effects similar to Aacec B-knockdown. The Aacec B peptide could be detected in both the cytoplasm and nuclei of pupal cells and was able to bind to the TTGG(A/C)A motif in AaPPO 3 DNA both in vitro and in vivo. These findings suggest that Aacec B plays a crucial role in pharate adult cuticle formation via the regulation of AaPPO 3 gene expression in pupae.
Unraveling the molecular mechanism of photosynthetic toxicity of highly fluorescent silver nanoclusters to Scenedesmus obliquus
Authors: Zhang Li , Goswami Nirmal , Xie Jianping , Zhang Bo , He Yiliang 
Published: November 2017

AbstractWhile the discovery of numerous attractive properties of silver at the nanoscale has increased their demand in many sectors including medicine, optics, sensing, painting and cosmetics, it has also raised wide public concerns about their effect on living organisms in aquatic environment. Despite the continuous effort to understand the various aspects of the toxicity of silver nanomaterials, the molecular level understanding on their cytotoxicity mechanism to biological organisms has remained unclear. Herein, we demonstrated the underlying mechanism of the photosynthetic toxicity against green algae namely, Scenedesmus obliquus by using an emerging silver nanomaterial, called silver nanoclusters (defined as r-Ag NCs). By exploiting the unique fluorescence properties of r-Ag NCs along with various other analytical/biological tools, we proposed that the photosynthetic toxicity of r-Ag NCs was largely attributed to the “joint-toxicity” effect of particulate form of r-Ag NCs and its released Ag+, which resulted in the disruption of the electron transport chain of light reaction and affected the content of key enzymes (RuBP carboxylase/ oxygenase) of Calvin cycle of algae cells. We believe that the present study can also be applied to the assessment of the ecological risk derived from other metal nanoparticles.