bluuebunny/crossref_metadata_2025_split huggingface dataset:
Erythrosis Pigmentosa Peribuccalis in an Adolescent: Dermoscopic Description and Management
Authors: Luna Paula Carolina , Abad María Eugenia , González Virginia M. , Vigovich Felix , Casas José Gabriel , Larralde Margarita 
Published: January 2016

AbstractErythrosis pigmentosa peribuccalis is an infrequent condition, with fewer than 35 cases reported in the literature, that affects mainly women. It presents as small papules that form a hyperpigmented plaque around the mouth and nose. Little is known about the etiology of this condition, its dermoscopic characteristics have not been described, and no effective treatment has been reported. We report a 15‐year‐old girl with this rare dermatosis who was successfully managed with daily calcipotriol, weekly topical ivermectin, and strict photoprotection. We also describe for the first time the dermoscopic findings of this entity.
“EB, or Not EB?” Neonatal Desquamative Impetigo in a Degloving Pattern
Authors: Nguyen Tuyet A. , Wang Audrey S. , Eichenfield Lawrence F. , Barrio Victoria 
Published: January 2016

AbstractWe present the case of a 7‐day‐old boy with significant, rapidly spreading blistering and desquamation in a “degloving” pattern on the hands that mimicked epidermolysis bullosa but was ultimately diagnosed as bullous impetigo caused by a clinically aggressive strain of Staphylococcus aureus. Bullous impetigo is a desquamating condition caused by local release of S. aureus exfoliative toxin A and is more commonly seen in children. This case highlights the fragility of newborn skin and reviews the major diagnoses that should be considered in an infant with significant blistering.
Congenital Panfollicular Nevus in a 6‐Month‐Old Girl
Authors: Duan Linna , Antaya Richard J. , Ko Christine J. , Narayan Deepak 
Published: January 2016

AbstractA 6‐month‐old girl presented with an irregular nodule on the right lateral neck. The lesion, which superficially resembled a lymphangioma or mastocytoma, had been present since birth but had recently been growing. The patient was referred for surgical resection. Microscopic findings were compatible with a recently described rare hamartoma termed “panfollicular nevus,” which is a well‐circumscribed lesion containing aberrantly differentiated, clustered hair follicles in high density.
Successful cardiac transplantation in a patient with congenital generalized lipodystrophy
Authors: Marcos‐Alonso Sonia , Bautista‐Hernandez Víctor , Portela Torrón Francisco 
Published: January 2016

AbstractWe report a case of a 12‐yr‐old boy referred to our unit with congenital generalized lipodystrophy and dilated cardiomyopathy related to a lamin gene mutation. He progressively developed end‐stage heart failure and was referred for heart transplant evaluation. The patient's lipid profile, glucose level, and renal function were normal, and vascular retinopathy was ruled out. He underwent orthotopic bicaval HT and had an uneventful recovery. He was discharged home two wk after surgery with good graft function. During follow‐up, he developed hyperglycemia and dyslipidemia, which were controlled by increasing leptin dose and starting oral antidiabetic drugs. The patient is currently doing well two yr after transplantation.
Supporting SIDS Families: The Public Health Nurse SIDS Home Visit
Authors: Stastny Penny F. , Keens Thomas G. , Alkon Abbey 
Published: January 2016

AbstractSudden infant death syndrome (SIDS) death has a devastating effect on parents. There is no known cause, so parents experience guilt about what they might have done or not done to contribute to the death. Although some SIDS parents may receive support from family and friends, the public health nurse (PHN) has an important professional role in providing grief support, SIDS education, and offering SIDS resources and referrals. Based on years of clinical practice, we recommend the following: Perform the home visit as soon as possible. Show care and compassion. Personalize the baby by using his or her name and asking to see photographs. Reassure the parents that grief is a process which takes time. Educate about what SIDS is and what it is not. Increasingly, SIDS deaths occur in the presence of risk factors. Explain that risk factors are not causes of death. As an authority in health care, reassuring families that they did not cause their baby's death has a tremendous impact on relieving guilt. Putting newly bereaved SIDS parents in contact with other SIDS parents is one of the most helpful actions a PHN can take to help families.
Massive haemorrhage protocol: what's the best protocol?
Authors: Callum J. L. , Nascimento B. , Alam A. 
Published: January 2016

Massive transfusion protocols became common practice between 2006 and 2010. The terminology of ‘massive transfusion protocol’ was improved to ‘massive haemorrhage protocol (MHP)’ with the astute recognition that at the start of such a protocol, it is unclear which patients will meet the definition of massive transfusion (10 U/24 h). Despite the majority of the literature being reported from the trauma population, hospitals have generally adopted a single MHP for all patients. It remains unclear whether the same protocol can be used for all patients. MHPs assist with the prevention and management of the acute coagulopathy of trauma/shock (ACOTS). The goals of a MHP are to improve haemostasis, communication and patient outcomes. The protocol must be specific for an individual hospital, depending on factors such as prehospital transport times, distance from laboratory to trauma and operating rooms, patient populations served and types of tests available. The key components of a MHP are the 6Ts: triggering of the protocol, laboratory testing, tranexamic acid, temperature maintenance, transfusion support and termination of the protocol when haemostasis is achieved. The evidence and importance of each of these steps will be discussed in detail. It is also critical that a quality assurance programme supports the MHP. Poor compliance with the institutional MHP is associated with inferior survival. Each MHP activation should be followed by a formal debrief by the team. Audits should be performed to determine compliance and to inform annual update of the MHP. Formal training and/or simulation should be a core part of the policy.
Transfusion triggers in patients with postpartum haemorrhage
Authors: Prick B. W. , Duvekot J. J. , van Rhenen D. J. , Jansen A. J. G. 
Published: January 2016

Postpartum haemorrhage (PPH) is one of the top five causes of maternal mortality both in developed and developing countries. In the Netherlands, we reported an incidence of severe PPH (defined as ≥1000 ml blood loss) of 4·5% in the period 2000–2008. As the majority of women who experience PPH have no identifiable clinical or historical risk factors, all women must be considered at risk and active third‐stage management is recommended in all women to prevent PPH. Red blood cell (RBC) transfusion is often necessary in the treatment of women who suffered from PPH. However, the current awareness of transfusion‐related complications (blood‐borne infections, transfusion reactions and immunologic effects) and the cost prompted a re‐evaluation of the transfusion practice. With only small differences in physical fatigue scores and no differences in secondary outcomes, we recommend implementation of a restrictive transfusion policy for women with PPH without severe anaemic complaints. This restrictive transfusion policy saves €438 per woman compared to a liberal transfusion policy. In addition, we recommend implementation of health‐related quality of life scores for counselling and decision‐making in clinical practice regarding transfusion policy after PPH.
Intra-articular glenohumeral injections of HYADD®4-G for the treatment of painful shoulder osteoarthritis: a prospective multicenter, open-label trial
Authors: Porcellini Giuseppe , Merolla Giovanni , Giordan Nicola , Paladini Paolo , Burini Andrea , Cesari Eugenio , Castagna Alessandro 
Published: January 2016

Purpose: numerous experimental and clinical studies in osteoarthritis (OA) have demonstrated that intraarticular (IA) administration of hyaluronic acid can improve the altered rheological properties of the synovial fluid and exert protective and reparative effects on the joint structure. The objective of this study was to evaluate the safety and performance of HYADD®4-G (Hymovis®) in patients with glenohumeral joint OA.
Methods: forty-one patients with shoulder pain and limited shoulder function resulting from concentric glenohumeral joint OA were enrolled in a multicenter clinical trial. Patients received two HYADD®4-G injections administered one week apart. The main outcome measure was improvement in shoulder pain on movement at six months as assessed through a 100-mm visual analog scale (VAS), range of motion (ROM) values, and Constant-Murley Shoulder Outcome Score (CS).
Results: two IA injections of HYADD®4-G (Hymovis®) significantly decreased pain and improved shoulder function for up to six months from the first injection. The VAS score decreased (from 66.1 mm to 37.7 mm at six months) and improvements were recorded in the total CS and in the ROM values ( rotation decreased from a mean value of 54.2° at baseline to 63.2° at six months and internal rotation from a mean value of 44.0° at baseline to 45.7° at 26 weeks). No serious adverse events occurred.
Conclusions: the study results demonstrated that two IA injections of HYADD®4-G (Hymovis®) may be a safe and effective treatment option for shoulder pain associated with glenohumeral OA and that the effects of the injections are still present for up to six months after the treatment.
Level of evidence: Level IV, therapeutic case series.
Early joint degeneration and antagonism between growth factors and reactive oxygen species. Is non-surgical management possible?
Authors: Manunta Andrea , Zedde Pietro , Cudoni Sebastiano , Caggiari Gianfilippo , Pintus Gianfranco 
Published: January 2016

Purpose: in pathological conditions such as osteoarthritis (OA), overproduction of reactive oxygen species (ROS) may overwhelm the antioxidant defenses of chondrocytes, thus promoting oxidative stress and cell death. It can be hypothesized that increasing the antioxidant machinery of chondrocytes may prevent the age-associated progression of this disease. Growth factors (GFs) play an important role in promoting both the resolution of inflammatory processes and tissue repair. in view of these considerations, we set out to investigate the protective effect, against H2O2-induced oxidative cell death, potentially exerted by fluid drained from the joint postoperatively.
Methods: the present study was conducted in 20 patients diagnosed with bilateral knee osteoarthritis and treated, between January 2013 and June 2014, with prosthetic knee implantation on the side more affected by the arthritic process, together with intraoperative placement of a closed-circuit drainage aspiration system. As a result, 20 different serum samples were collected from the drained articular fluid, prepared using two different methodologies. In addition, forty blood serum samples were obtained and prepared: 20 from the surgically treated patients and 20 from healthy controls. The present work was undertaken to investigate the potential protective effect of sera obtained from articular fluid drainage against hydrogen peroxide-induced oxidative stress in cultured human chondrocytes.
Results: exposure of chondrocytes to hydrogen peroxide elicited a dose-dependent increase in oxidative stress and chondrocyte cell death, phenomena that were significantly counteracted by the pre-treatment of cell cultures with sera from articular fluid drainage.
Conclusions: oxidatively stressed chondrocytes treated with sera obtained from articular fluid drainage lived longer than those treated with blood serum samples and longer than untreated ones.
Clinical Relevance: synovial fluids are usually discarded once the drainage reservoir is full; instead they could benefit the patients from whom they are collected, as they are rich in growth factors and they may act as antagonists of ROS effects. Accordingly, they could be used to treat chondropathies, early OA, and mild OA located in other sites.
Results of meniscectomy and meniscal repair in anterior cruciate ligament reconstruction
Authors: Deledda Davide , Rosso Federica , Cottino Umberto , Bonasia Davide , Rossi Roberto 
Published: January 2016

Meniscal tears are commonly associated with anterior cruciate ligament (ACL) injuries. A deficient medial meniscus results in knee instability and could lead to higher stress forces on the ACL reconstruction.Comparison of results in meniscectomy and meniscal repairs revealed worse clinical outcomes in meniscectomy, but higher re-operation rates in meniscal repairs. Our aim was to review the results of ACL reconstruction associated with meniscectomy or meniscal repair.
Draft Genome Sequences of Nine Strains of Ralstonia solanacearum Differing in Virulence to Eggplant (
Solanum melongena
)
Authors: Guinard Jérémy , Vinatzer Boris A. , Poussier Stéphane , Lefeuvre Pierre , Wicker Emmanuel 
Published: January 2016

ABSTRACT

Ralstonia solanacearum
displays variability in its virulence to solanaceous crops. We report here the draft genome sequences of eight phylotype I strains and one phylotype III strain differing in virulence to the resistant eggplant genotype AG91-25. These data will allow the identification of virulence- and avirulence-related genes.
Draft Genome Sequences of Five Pseudomonas aeruginosa Clinical Strains Isolated from Sputum Samples from Cystic Fibrosis Patients
Authors: Couger M. B. , Wright Anna , Lutter Erika I. , Youssef Noha 
Published: January 2016

ABSTRACT

We report here the draft genome sequences of five
Pseudomonas aeruginosa
isolates obtained from sputum samples from two cystic fibrosis patients with chronic colonization. These closely related strains harbor 225 to 493 genes absent from the
P. aeruginosa
POA1 genome and contain 178 to 179 virulence factors and 29 to 31 antibiotic resistance genes.
Identification and Complete Genome Sequence Analysis of a Genotype XIV Newcastle Disease Virus from Nigeria
Authors: Shittu Ismaila , Sharma Poonam , Volkening Jeremy D. , Solomon Ponman , Sulaiman Lanre K. , Joannis Tony M. , Williams-Coplin Dawn , Miller Patti J. , Dimitrov Kiril M. , Afonso Claudio L. 
Published: January 2016

ABSTRACT
The first complete genome sequence of a strain of Newcastle disease virus (NDV) from genotype XIV is reported here. Strain duck/Nigeria/NG-695/KG.LOM.11-16/2009 was isolated from an apparently healthy domestic duck from a live bird market in Kogi State, Nigeria, in 2009. This strain is classified as a member of subgenotype XIVb of class II.
Whole-Genome Sequence of Duck Tembusu Virus Strain DTMUV/CH/2014, Isolated in China
Authors: Zhou Xinrong , Zhang Tiansheng , Song Deping , Huang Tao , Peng Qi , Chen Yanjun , Li Anqi , Zhang Fanfan , Wu Qi , Ye Yu , Tang Yuxin 
Published: January 2016

ABSTRACT
The whole-complete genome sequence of a strain of duck tembusu virus (DTMUV), DTMUV/CH/2014, affecting layer ducks in China, was determined and characterized. Compared with DTMUV sequences available in GenBank, DTMUV/CH/2014 has 3 amino acid mutations located in the capsid, prM, and NS3 genes of DTMUV/CH/2014.
Genome Sequence of Bovine Polyomavirus 1 Detected in a Salers Cow (Bos taurus) from Catalonia, Spain
Authors: Ben Salem Nicole , Pérez de Val Bernat , Martin Maite , Moens Ugo , Ehlers Bernhard 
Published: January 2016

ABSTRACT

We identified a variant of the first bovine polyomavirus (BPyV1; family
Polyomaviridae
) in a lymph node of a Salers cow. As the 2 previously published genome sequences of this virus originated from fetal bovine serum and ground beef, respectively, this is the first BPyV1 genome that could be traced back to an individual.
Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain PA01, Isolated from Sheep in Pará, Brazil
Authors: Alves Jorianne T. C. , Veras Adonney A. O. , Cavalcante Ana Lídia Q. , de Sá Pablo H. C. G. , Dias Larissa M. , Guimarães Luis C. , Morais Ezequiel , Silva André G. M. , Azevedo Vasco , Ramos Rommel T. J. , Silva Artur , Carneiro Adriana R. 
Published: January 2016

ABSTRACT

Corynebacterium pseudotuberculosis
is the etiological agent of caseous lymphadenitis disease. In this work, we present the first complete genome sequence of
Corynebacterium pseudotuberculosis
strain PA01, isolated in northern Brazil from an infected sheep. The genome length is 2,337,920 bp, and 2,003 coding sequences (CDS), 12 rRNAs, and 49 tRNAs were predicted.
Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae
Authors: Bossé Janine T. , Chaudhuri Roy R. , Li Yanwen , Leanse Leon G. , Fernandez Crespo Roberto , Coupland Paul , Holden Matthew T. G. , Bazzolli Denise M. , Maskell Duncan J. , Tucker Alexander W. , Wren Brendan W. , Rycroft Andrew N. , Langford Paul R. 
Published: January 2016

ABSTRACT

We report here the complete annotated genome sequence of a clinical serovar 8 isolate
Actinobacillus pleuropneumoniae
MIDG2331. Unlike the serovar 8 reference strain 405, MIDG2331 is amenable to genetic manipulation via natural transformation as well as conjugation, making it ideal for studies of gene function.
Identification of Proteins Bound to Dengue Viral RNA
In Vivo
Reveals New Host Proteins Important for Virus Replication
Authors: Phillips Stacia L. , Soderblom Erik J. , Bradrick Shelton S. , Garcia-Blanco Mariano A. 
Published: January 2016

ABSTRACT

Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. Host RNA-binding proteins mediate various aspects of virus replication through their physical interactions with viral RNA. Here we describe a technique designed to identify such interactions in the context of infected cells using UV cross-linking followed by antisense-mediated affinity purification and mass spectrometry. Using this approach, we identified interactions, several of them novel, between host proteins and dengue viral RNA in infected Huh7 cells. Most of these interactions were subsequently validated using RNA immunoprecipitation. Using small interfering RNA (siRNA)-mediated gene silencing, we showed that more than half of these host proteins are likely involved in regulating virus replication, demonstrating the utility of this method in identifying biologically relevant interactions that may not be identified using traditional
in vitro
approaches.

IMPORTANCE
Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Viral RNA molecules physically interact with cellular RNA-binding proteins (RBPs) throughout the course of infection; the identification of such interactions will lead to the elucidation of the molecular mechanisms of virus replication. Until now, the identification of host proteins bound to dengue viral RNA has been accomplished using
in vitro
strategies. Here, we used a method for the specific purification of dengue viral ribonucleoprotein (RNP) complexes from infected cells and subsequently identified the associated proteins by mass spectrometry. We then validated a functional role for the majority of these proteins in mediating efficient virus replication. This approach has broad relevance to virology and RNA biology, as it could theoretically be used to purify any viral RNP complex of interest.
The Dual MEK/FLT3 Inhibitor E6201 Exerts Cytotoxic Activity against Acute Myeloid Leukemia Cells Harboring Resistance-Conferring FLT3 Mutations
Authors: Zhang Weiguo , Borthakur Gautam , Gao Chen , Chen Ye , Mu Hong , Ruvolo Vivian R. , Nomoto Kenichi , Zhao Nanding , Konopleva Marina , Andreeff Michael 
Published: January 2016

Abstract
Fms-like tyrosine kinase 3 (FLT3) inhibition has elicited encouraging responses in acute myeloid leukemia (AML) therapy. Unfortunately, unless combined with a bone marrow transplant, disease relapse is frequent. In addition to the acquired point mutations in the FLT3 kinase domain that contribute to FLT3 inhibitor resistance, MEK/ERK signaling is persistently activated in AML cells even when FLT3 phosphorylation is continually suppressed. Thus, concomitant targeting of FLT3 and MAPK may potentially exert synergistic activity to counteract the resistance of AML cells to FLT3-targeted therapy. In this study, we investigated the antileukemia activity of a MEK1 and FLT3 dual inhibitor, E6201, in AML cells resistant to FLT3 inhibition. We found that E6201 exerted profound apoptogenic effects on AML cells harboring resistance-conferring FLT3 mutations. This activity appeared to be p53 dependent, and E6201-induced cytotoxicity was retained under hypoxic culture conditions and during coculture with mesenchymal stem cells that mimic the AML microenvironment. Furthermore, E6201 markedly reduced leukemia burden and improved the survival of mice in a human FLT3–mutated AML model. Collectively, our data provide a preclinical basis for the clinical evaluation of E6201 in AML patients harboring FLT3 mutations, including those who relapse following FLT3-targeted monotherapy. Cancer Res; 76(6); 1528–37. ©2016 AACR.
Small RNAs Recruit Chromatin-Modifying Enzymes MMSET and Tip60 to Reconfigure Damaged DNA upon Double-Strand Break and Facilitate Repair
Authors: Wang Qinhong , Goldstein Michael 
Published: January 2016

Abstract
Recent reports have demonstrated that DNA double-strand break (DSB)–induced small RNAs (diRNA) play an important role in the DNA damage response (DDR). However, the molecular mechanism by which diRNAs regulate the DDR remains unclear. Here, we report that Dicer- and Drosha-dependent diRNAs function as guiding molecules to promote the recruitment of the methyltransferase MMSET (WHSC1) and the acetyltransferase Tip60 (KAT5) to the DSB, where local levels of histone H4 di- and tri-methylation at lysine 20 (H4K20me2, 3) and H4 acetylation at lysine 16 (H4K16Ac) were enhanced. These histone modification events resulted in an open, flexible chromatin configuration, as indicated by the increased release of histones γH2AX, H2AX, and H3 from damaged chromatin. Furthermore, we found that diRNA-associated AGO2 interacted with MMSET and Tip60 and that the diRNA binding and catalytic activities of AGO2 were dispensable for the interaction but required for the recruitment of MMSET and Tip60 to DSBs. Consequently, diRNA-mediated chromatin remodeling promoted DSB repair by enhancing the recruitment of Rad51 and BRCA1 to the DSB site. Taken together, our findings reveal an unexpected direct role for diRNAs in regulating chromatin remodeling to facilitate DSB repair, revealing a new layer of DDR regulation involving specialized RNA molecules. Cancer Res; 76(7); 1904–15. ©2016 AACR.