bluuebunny/crossref_metadata_2025_split huggingface dataset:
Current Advances in Highly Multiplexed Antibody‐Based Single‐Cell Proteomic Measurements
Authors: Huang Lu , Michael Sean A. , Chen Yangfan , Wu Hongkai 
Published: May 2017

AbstractSingle‐cell measurements have played a critical role in revealing the complex signaling dynamics and heterogeneity present in cells, but there is still much to learn. Measuring samples from bulk populations of cells often masks the information and dynamics present in subsets of cells. Common single‐cell protein studies rely on fluorescent microscopy and flow cytometry but are limited in multiplexing ability owing to spectral overlap. Recently, technology advancements in single‐cell proteomics have allowed highly multiplexed measurement of multiple parameters simultaneously by using barcoded microfluidic enzyme‐linked immunosorbent assays and mass cytometry techniques. In this review, we will describe recent work around multiparameter single‐cell protein measurements and critically analyze the techniques.
UHPLC‐ESI‐MS/MS determination and pharmacokinetics of pinoresinol glucoside and chlorogenic acid in rat plasma after oral administration of Eucommia ulmoides Oliv extract
Authors: Gong Xiaojian , Luan Qingxiang , Zhou Xin , Zhao Yang , Zhao Chao 
Published: May 2017

AbstractThis study aimed to develop a specific UHPLC‐ESI‐MS/MS method for simultaneous determination and pharmacokinetics of pinoresinol glucoside and chlorogenic acid in rat plasma after oral administration of Eucommia ulmoides. The chromatographic separation was achieved on a Hypersil GOLD column with gradient elution by using a mixture of 0.1% formic acid aqueous solution and acetonitrile as the mobile phase at a flow rate of 200 μL/min. A tandem mass spectrometric detection was conducted using multiple‐reaction monitoring via an electrospray ionization source in negative ionization mode. Samples were pre‐treated by a single‐step protein precipitation with acetonitrile, and bergenin was used as internal standard. After oral administration of 3 mL/kg E. ulmoides extract in rats, the maximum plasma concentrations of pinoresinol glucoside and chlorogenic acid were 57.44 and 61.04 ng/mL, respectively. The times to reach the maximum plasma concentration were 40.00 and 23.33 min for pinoresinol glucoside and chlorogenic acid, respectively. The intra‐ and inter‐day precision (RSD) values for the two analytes were <2.46 and 5.15%, respectively, and the accuracy (RE) values ranged from −12.76 to 0.00. This is the first study on pharmacokinetics of bioactive compounds in rat plasma after oral administration of E. ulmoides extract.
Predicting the optimum compositions of a transdermal nanoemulsion system containing an extract of Clinacanthus nutans leaves (L.) for skin antiaging by artificial neural network model
Authors: Che Sulaiman Intan Soraya , Basri Mahiran , Fard Masoumi Hamid Reza , Ashari Siti Efliza , Basri Hamidon , Ismail Maznah 
Published: May 2017

A nanoemulsion‐based formulation containing leaf extracts of Clinacanthus nutans Lindau (C. nutans) was prepared for therapeutic use and optimized by artificial neural network (ANN). The model was applied to optimize the particle size of the transdermal nanoemulsion system containing an extract of C. nutans leaves for skin antiaging. Five universal learning algorithms—incremental back propagation, batch back propagation, quick propagation, genetic algorithm, and Levenberg‐Marquardt—were used in the ANN to achieve the optimum topologies. The optimum topologies were selected among the learning algorithms trained with lowest root mean square values. Genetic Algorithm‐5‐13‐1 was found to be the optimum topology for the final model to predict the optimum particle size. Under these conditions, the actual particle size of the optimum nanoemulsion compared well with the predicted values with the residual standard error of less than 1.5%. The final formulation was observed to be stable against phase separation under an accelerated stability study and during storage at room temperature and 45°C for 90 days. The dermal irritation assay demonstrated that the developed nanoemulsion was found to be compatible with human skin and could be classified as nonirritant. In vivo ultrasound attributes of the skin study showed that the collagen content increased significantly with the application of the C. nutans nanoemulsion among all 21 volunteers during the 21 days of the treatment period. Hence, ANN was found to be an effective tool for optimizing the nanoemulsion containing C. nutans leaves extract with efficacy to protect collagen from breakdown.
Highly Active and Iso‐Selective Catalysts for the Ring‐Opening Polymerization of Cyclic Esters using Group 2 Metal Initiators
Authors: Bhattacharjee Jayeeta , Harinath Adimulam , Nayek Hari Pada , Sarkar Alok , Panda Tarun K. 
Published: May 2017

AbstractA series of alkali and alkaline earth (Ae) metal complexes bearing 1,2‐phenylene(bis‐diphenylphosphinothioic/selenoic amine) [{Ph2P(E)NH}2C6H4] (E=S (1‐H2); Se (2‐H2) ligands are reported. Alkali metal complexes [{Ph2P(S)N}2C6H4]Na(THF)4 (3 a) [{Ph2P(Se)N}2C6H4]Na(THF)4 (3 b), and [{Ph2P(Se)N}2C6H4]K(THF)5 (4 b) were obtained in good yield by treating protic ligands 1‐H2 or 2‐H2 with metal hexamethyldisilazides [MN(SiMe3)2] (M=Na or K) at ambient temperature. The Ae metal complexes formulated as [{Ph2P(E)N}2C6H4]M(THF)3 [E=S, M=Ca (5 a), Sr (6 a), Ba (7 a); E=Se, M=Ca (5 b), Sr (6 b), Ba (7 b)] can be synthesized by using two routes. The molecular structures of the free ligand 1‐H2 and metal complexes 5 a,b–7 a,b in their solid states were established. Complexes 3 a and 3 b are isostructural; however, in complex 4 b, an attachment different from ligand 2 was observed. The complexes 5 a,b–7 a,b are isostructural and each metal ion exhibits a distorted pentagonal bipyramidal geometry around it. All Ae metal complexes 5 a,b–7 a,b were tested for the ring‐opening polymerization (ROP) of racemic lactide (rac‐LA) and ϵ‐caprolactone (ϵ‐CL) at room temperature. Calcium complexes 5 a and 5 b show excellent iso‐selectivity, with Pi values of 0.78–0.87 at 298 K and with a high degree of polymerization control, whereas the corresponding strontium complexes 6 a and 6 b exhibit moderate iso‐selectivity, and barium complexes 7 a and 7 b yield only atactic polylactides (PLAs). In all cases, the catalyst initiates the ROP catalytic cycle in the absence of any external initiator. Kinetic studies of the polymerization reactions indicate the relative order of polymerization rate increases with increase in the size of the metal ion: Ba>Sr>Ca.
Coordination Behavior of [Cp′′2Zr(η1:1‐P4)] towards Different Lewis Acids
Authors: Seitz Andreas E. , Vogel Ulf , Eberl Miriam , Eckhardt Maria , Balázs Gábor , Peresypkina Eugenia V. , Bodensteiner Michael , Zabel Manfred , Scheer Manfred 
Published: May 2017

AbstractA detailed method for the preparation of [Cp′′2Zr(η1:1‐P4)] (1) is presented. The coordination behavior of 1 towards Lewis acidic transition metal complexes of tungsten, manganese, and iron, respectively, and main group compounds (AlMe3, AlEt3) was investigated in detail by computational and experimental studies. In doing so, a series of unprecedented complexes with different coordination modes and multiple coordination numbers of the tetraphosphabicyclo[1.1.0]butane framework were synthesized. All products, as well as the starting materials, were comprehensively characterized by NMR spectroscopy, mass spectrometry, elemental analysis, and single crystal X‐ray structural analysis.
Direct Observation of Confined I−⋅⋅⋅I2⋅⋅⋅I− Interactions in a Metal–Organic Framework: Iodine Capture and Sensing
Authors: Hu Yue‐Qiao , Li Mu‐Qing , Wang Yanyan , Zhang Tao , Liao Pei‐Qin , Zheng Zhiping , Chen Xiao‐Ming , Zheng Yan‐Zhen 
Published: May 2017

AbstractHerein a strategy is reported for capturing and sensing iodine by strong I−⋅⋅⋅I2⋅⋅⋅I− interaction, confined in a metal‐organic framework, [Tb(Cu4I4)(ina)3(DMF)] (1) (ina=isonicotinate). As revealed by single‐crystal X‐ray crystallography, the uptaken I2 molecules directly contact the {Cu4I4}n chains, virtually forming an electronically polarizable tetraiodide anion (I42−) through strong I−⋅⋅⋅I2⋅⋅⋅I− interaction. As such, a quasi‐copper‐iodide layer of {Cu4I5}n with semiconducting characteristics results, leading to a significant enhancement (Δσ=107 times) in electrical conductivity over the I2‐free 1. The effect observed is several orders of magnitude higher than those reported due to iodine⋅⋅⋅aromatic interactions (Δσ=102 times) and by interactions between I2 and a redox‐active metal centre (Δσ=104 times). The drastic enhancement in electrical conductivity was used to switch on/off an LED bulb, suggesting the possibility of electrically sensing I2.
Elektrotauchlackierung
Authors: Brock Thomas 
Published: May 2017

ZusammenfassungDie Elektrotauchlackierung ist ein modernes Lackierverfahren zur organischen Beschichtung insbesondere von komplex geformten Metallteilen mittels elektrischen Gleichstroms. Das Prinzip der Elektrotauchlackierung beruht auf der Entladung und sich anschließenden Abscheidung von Polymerionen auf einem als Elektrode dienenden metallenen Untergrund. Das nachfolgende Einbrennen mit physikalischer Filmbildung und chemischer Vernetzung führt zu einem hervorragendem Korrosionsschutz und weiteren chemischen und mechanischen Schutzeigenschaften. Die extrem gleichmäßige Schichtausbildung sogar in Hohlräumen beruht auf dem zunehmenden elektrischen Widerstand von abgeschiedenem und verdichtetem Lackmaterial auf der Metalloberfläche.
Identification of α7 Nicotinic Acetylcholine Receptor Silent Agonists Based on the Spirocyclic Quinuclidine‐Δ2‐Isoxazoline Scaffold: Synthesis and Electrophysiological Evaluation
Authors: Quadri Marta , Matera Carlo , Silnović Almin , Pismataro Maria Chiara , Horenstein Nicole A. , Stokes Clare , Papke Roger L. , Dallanoce Clelia 
Published: May 2017

AbstractCompound 11 (3‐(benzyloxy)‐1′‐methyl‐1′‐azonia‐4H‐1′‐azaspiro[isoxazole‐5,3′‐bicyclo[2.2.2]octane] iodide) was selected from a previous set of nicotinic ligands as a suitable model compound for the design of new silent agonists of α7 nicotinic acetylcholine receptors (nAChRs). Silent agonists evoke little or no channel activation but can induce the α7 desensitized Ds state, which is sensitive to a type II positive allosteric modulator, such as PNU‐120596. Introduction of meta substituents into the benzyloxy moiety of 11 led to two sets of tertiary amines and quaternary ammonium salts based on the spirocyclic quinuclidinyl‐Δ2‐isoxazoline scaffold. Electrophysiological assays performed on Xenopus laevis oocytes expressing human α7 nAChRs highlighted four compounds that are endowed with a significant silent‐agonism profile. Structure–activity relationships of this group of analogues provided evidence of the crucial role of the positive charge at the quaternary quinuclidine nitrogen atom. Moreover, the present study indicates that meta substituents, in particular halogens, on the benzyloxy substructure direct specific interactions that stabilize a desensitized conformational state of the receptor and induce silent activity.
Palmar‐plantar erythrodysesthesia syndrome following treatment with high‐dose methotrexate or high‐dose cytarabine
Authors: Karol Seth E. , Yang Wenjian , Smith Colton , Cheng Cheng , Stewart Clinton F. , Baker Sharyn D. , Sandlund John T. , Rubnitz Jeffrey E. , Bishop Michael W. , Pappo Alberto S. , Jeha Sima , Pui Ching‐Hon , Relling Mary V. 
Published: May 2017

BACKGROUNDPalmar‐plantar erythrodysesthesia syndrome (PPES) is an uncommon side effect of high‐dose cytarabine or methotrexate. Prior case reports of PPES have been limited, and the predisposing factors for the development of PPES remain unknown.METHODSA review of databases identified 22 patients (1.3%) who developed 39 episodes of PPES among 1720 patients after treatment with high‐dose cytarabine or methotrexate.RESULTSSymptoms lasted a mean of 6.4 days. Hands and feet were both involved in 68% of the initial episodes. Parenteral opioids were required for pain control by 27% of the patients. In comparison with the 1698 children treated with similar therapy, the children who developed PPES were older (mean age at diagnosis, 14.3 vs 7.7 years; P = 7.5 × 10–7). The frequency of PPES was less common in patients receiving methotrexate alone (7 of 946 or 0.7%) versus cytarabine (7 of 205 or 3.4%; P = .005) but was not different for those receiving both high‐dose methotrexate and cytarabine (8 of 569 or 1.4%; P = .32). Prolonged infusions of methotrexate were associated with less frequent PPES in comparison with rapid infusions (P = 1.5 × 10–5), as was the co‐administration of dexamethasone with cytarabine (P = 2.5 × 10–6). Self‐described race and sex were not associated with PPES. In a multivariate analysis, older age and high‐dose cytarabine administration without dexamethasone remained associated with PPES (P = 1.1 × 10–4 and P = .038, respectively). A genome‐wide association study did not identify any associations with PPES meeting the genome‐wide significance threshold, but top variants were enriched for skin expression quantitative trait loci, including rs11764092 in AUTS2 (P = 6.45 × 10–5).CONCLUSIONSThese data provide new insight into the incidence of PPES as well as its risk factors. Cancer 2017;123:3602‐8. © 2017 American Cancer Society.
Sudden gains in exposure‐focused cognitive‐behavioral group therapy for panic disorder
Authors: Nogueira‐Arjona Raquel , Santacana Martí , Montoro María , Rosado Silvia , Guillamat Roser , Vallès Vicenç , Fullana Miquel A. 
Published: May 2017

In the context of psychological treatment, a sudden gain is a large and enduring improvement in symptom severity that occurs between two single therapy sessions. The influence of sudden gains on long‐term outcomes and functional impairment in anxiety disorders is not well understood, and little is known with regard to panic disorder in particular. In addition, previous research on patients with anxiety disorders has produced inconsistent results regarding the relationship between sudden gains and cognitive change. We examined the incidence of sudden gains in a large sample (n = 116) of panic disorder patients undergoing exposure‐focused cognitive‐behavioral group therapy, and compared panic severity, functional impairment, and cognitive change in patients with and without sudden gains at posttreatment and 6‐month follow‐up. Participants who experienced sudden gains displayed lower levels of panic severity and functional impairment at posttreatment and 6‐month follow‐up than those who did not experience sudden gains. However, we observed no difference in cognitive changes between groups, either at posttreatment or at follow‐up. Our results demonstrate that the beneficial effects of sudden gains on therapeutic outcomes not only extend to long‐term and functional outcome measures but are also evident in less cognitive (i.e., exposure‐focused) forms of psychological treatment.Key Practitioner Message

Sudden gains are common in panic disorder patients undergoing exposure‐based cognitive‐behavioral group therapy.
Sudden gains during exposure‐focused therapy are linked to greater improvement in panic disorder severity and functional impairment.
The positive impact of sudden gains on panic disorder severity and functional impairment is maintained in the long term.
Nature‐Inspired Synthesis of Nanostructured Electrocatalysts through Mineralization of Calcium Carbonate
Authors: Ko Jong Wan , Son Eun Jin , Park Chan Beum 
Published: May 2017

AbstractBiomineralization is a biogenic process that produces elaborate inorganic and organic hybrid materials in nature. Inspired by the natural process, this study explored a new mineralization approach to create nanostructured CaCO3films composed of amorphous CaCO3hemispheres by using catechol‐rich polydopamine (PDA) as a biomimetic mediator. The thus synthesized biomimetic CaCO3was successfully transformed to nanostructured films of metal‐oxide minerals, such as FeOOH, CoCO3, NiCO3, and MnOOH, through a simple procedure. The CaCO3‐templated metal‐oxide minerals functioned as efficient electrocatalysts; a CaCO3‐templated cobalt phosphate (nanoCoPi) film exhibited high stability as a water‐oxidation electrocatalyst with a current density of 1.5 mA cm−2. The nanostructure of nanoCoPi, consisting of individual nanoparticles (≈70 nm) and numerous internal pores (BET surface area: 3.17 m2 g−1), facilitated an additional charge‐transfer pathway from the electrode to individual active sites of the catalyst. This work demonstrates a plausible strategy for facile and green synthesis of nanostructured electrocatalysts through biomimetic CaCO3mineralization.
Developmental ecology of annual killifish Millerichthys robustus (Cyprinodontiformes: Cynolebiidae)
Authors: Domínguez‐Castanedo Omar , Valdesalici Stefano , Rosales‐Torres Ana María 
Published: May 2017

AbstractPopulations of annual killifishes persist in temporary water bodies over the dry season through the expression of diapause in their drought‐resistant embryos. Environmental cues may influence expression of the diapause phenotype during embryonic incubation. Millerichthys robustus is the only annual killifish distributed in North America. The aim of this review is to analyze the ecology of M. robustus development and contrast this with that of annual killifishes in austral locations. The temporary water bodies inhabited by M. robustus present the following environmental conditions: flood, drought, and humidity. During the flooding period, the environment presents the lowest temperatures, shortest photoperiod, and highest precipitation, and embryos were found in diapause I. The drought period features the highest temperatures and lowest precipitation, and embryos were found in diapause II. In contrast, during the humid period at the beginning of the rainy season, embryos were found in diapause I, II, and III, associated with the longer photoperiod and high temperatures. These dynamics of the diapause phenotypes can be explained by a combination of the strategies of phenotypic plasticity during flood and drought periods, and bet‐hedging during the humid period. Moreover, the microenvironmental conditions in which embryos were buried could influence developmental trajectories. Developmental Dynamics 246:802–806, 2017. © 2017 Wiley Periodicals, Inc.
Factors restricting the range expansion of the invasive green anoleAnolis carolinensison Okinawa Island, Japan
Authors: Suzuki‐Ohno Yukari , Morita Kenjiro , Nagata Nobuaki , Mori Hideaki , Abe Shintaro , Makino Takashi , Kawata Masakado 
Published: May 2017

AbstractThe green anoleAnolis carolinensisinvaded the Ogasawara Islands in Japan, drove various native species to extinction, and its distribution expanded 14 years after initial establishment.A. carolinensisinvaded Okinawa Island, but it has not expanded its distribution in more than 25 years, although its density is extremely high in the southern region. To determine whetherA. carolinensishas the potential to expand its distribution on Okinawa Island, we performed phylogenetic analysis of mitochondrialND2DNAsequences to study the origin ofA. carolinensisthat invaded Okinawa Island. We further used a species distribution model (MaxEnt) based on the distribution of native populations in North America to identify ecologically suitable areas on Okinawa Island. Nucleotide sequence analysis shows that the invaderA. carolinensisoriginated in the western part of the Gulf Coast and inland areas of the United States and that a portion of the anoles on Okinawa was not introduced via the Ogasawara Islands. The MaxEnt predictions indicate that most areas in Okinawa Island are suitable forA. carolinensis. Therefore,A. carolinensismay have the potential to expand its distribution in Okinawa Island. The predictions indicate that habitat suitability is high in areas of high annual mean temperature and urbanized areas. The values of precipitation in summer in the northern region of Okinawa Island were higher compared with those of North America, which reduced the habitat suitability in Okinawa Island. Adaptation to low temperatures, an increase in the mean temperature through global warming, and an increase in open environments through land development will likely expand the distribution ofA. carolinensisin Okinawa Island. Therefore, we must continue to monitor the introduced populations and be alert to the possibility that city planning that increases open environments may cause their range to expand.
Volatile markers of cholesterol thermal changes
Authors: Derewiaka Dorota , Zaręba Dorota , Obiedziński Mieczysław , Matuszewska‐Janica Aleksandra 
Published: May 2017

The goal of the study was to determine profile of volatile compounds and asses the markers of cholesterol transformations during thermal processing using SPME‐GC‐MS technique. The profile of volatile compounds of cholesterol is dependent on the temperature (120, 150, 180 and 220°C) and time (30, 60, 120 and 180 min) used during processing. The highest contents of volatile compounds were observed during thermal processing of cholesterol at 180 and 220°C. The most abundant group of volatiles were carbonyl compounds, for example, aldehydes, ketones and hydrocarbons. Statistical analysis of data allowed to point out markers of cholesterol changes like degradation, oxidation, polymerisation and formation of cholestadienes. 4‐methylpentan‐2‐ol was recognized as possible marker of cholesterol oxidation, 6‐methylheptan‐2‐one was markers of cholesterol degradation and 4‐mehyldec‐2‐ene and 5‐methylhexanal as markers of cholesterol polymerization. Volatile components that can be associated with formation of cholestadienes were 2‐pentanone and 2‐methylheptane.Practical application: The method reported in this study may be used for determination of volatile compounds in thermally processed sterol standards especially cholesterol. The developed method able us to determine the profile and content of volatiles, which were used during evaluation of cholesterol changes after thermal treatment. The reported data are crucial for assessment of markers of cholesterol degradation, for example, 6‐methylheptan‐2‐one or thermal oxidation, for example, was 4‐methylpentan‐2‐ol. Markers of cholesterol dimerization were 4‐methyldec‐2‐ene and 5‐methylhexanal. Moreover 2‐pentanone and 2‐methylheptane are volatiles which presence can be associated with formation of cholestadienes. This method can be used as evaluation method for changes of sterols, without a need of determination of sterols and other compounds like oxysterol, polymers of cholesterol and cholestadienes.During heating, cholesterol can undergo few processes for example degradation, polymerisation and oxidation. The aim of this study is to select volatile compounds, which can be used as markers of mentioned above processes. Due to performed SPME‐GC‐MS analysis of aroma components and other component like oxysterols, cholestadienes and cholesterol polymers (determined in previous study) and statistical analysis, it is possible to evaluate the volatile markers of particular processes for example 4‐methylpentan‐2‐one as marker of cholesterol oxidation, 6‐methylheptan‐2‐one as marker of cholesterol degradation and 5‐methylhexanal as dimerization marker.