bluuebunny/crossref_metadata_2025_split huggingface dataset:
spacedeconv: deconvolution of tissue architecture from spatial transcriptomics
Authors: Zackl Constantin , Zopoglou Maria , Stauffer Reto , Ausserhofer Markus , Ijsselsteijn Marieke E. , Sturm Gregor , de Miranda Noel Filipe da Cunha Carvalho , Finotello Francesca 
Published: October 2024

Abstract

Investigating tissue architecture is key to understanding tissue function in health and disease. While spatial omics technologies enable the study of cell transcriptomes within their native context, they often lack single-cell resolution. Deconvolution methods can computationally infer tissue composition from spatial transcriptomics data, but differences in their workflows complicate their use and comparison. We developed spacedeconv, a unified interface to different deconvolution methods that additionally supports data preprocessing, visualization, and analysis of cell communication and multimodal data. Here, we demonstrate how spacedeconv streamlines the investigation of the cellular and molecular underpinnings of tissue architecture in different organisms and tissue contexts.
Dissection of core promoter syntax through single nucleotide resolution modeling of transcription initiation
Authors: Danko Charles , He Adam 
Published: October 2024

Abstract

How the DNA sequence of cis-regulatory elements encode transcription initiation patterns remains poorly understood. Here we introduce CLIPNET, a deep learning model trained on population-scale PRO-cap data that predicts the position and quantity of transcription initiation with single nucleotide resolution from DNA sequence more accurately than existing approaches. Interpretation of CLIPNET revealed a complex regulatory syntax consisting of DNA-protein interactions in five major positions between -200 and +50 bp relative to the transcription start site, as well as more subtle positional preferences among transcriptional activators. Transcriptional activator and core promoter motifs work non-additively to encode distinct aspects of initiation, with the former driving initiation quantity and the latter initiation position. We identified core promoter motifs that explain initiation patterns in the majority of promoters and enhancers, including DPR motifs and AT-rich TBP binding sequences in TATA-less promoters. Our results provide insights into the sequence architecture governing transcription initiation.
A New Deterioration Mechanism for Icebergs
Authors: Cenedese Claudia , Ward Nash , McElwaine Jim , Condron Alan 
Published: October 2024

Abstract

Icebergs are a primary mechanism for the long-distance transport of terrestrial sediment in the marine environment. During the last ice age, icebergs transported sediment more than 2000 km across the North Atlantic. In addition, sediment plays a key role in marine nutrient fertilization, phytoplankton growth, and CO2 drawdown. However, the effect sediment has on the deterioration of icebergs, as well as rates of sediment loss to the marine environment, are poorly understood. Here we present a completely new mechanism for iceberg deterioration which has previously gone undiscovered. We conducted the first laboratory investigation into the effects of sediment on iceberg deterioration and found that it increases the melt rate in three ways. First, as expected, there is less ice to melt. Second, a sediment plume may form which enhances the heat flux towards the ice. Third, sediment layers induce an iceberg to break into multiple pieces, dramatically increasing the overall rate of ice melt. The latter mechanism has never been reported previously. Our results provide a parametrization that includes the effects of sediment on iceberg deterioration. Our findings have significant implications for more accurately modeling iceberg break-up and for the potential of icebergs melt water to trigger abrupt climate change.
High-performance Nano-PTFE reinforced nickel mold for defect-free micro injection molding of surface micro structures
Authors: Guan Tianyu , Su Quanliang , Song Rijian , Chen Yixin , Fang Fengzhou , Zhang Nan 
Published: October 2024

Abstract

Interest in electroformed nickel (Ni) molds has continued to increase due to their high precision, low cost and high surface finish. Nevertheless, pure Ni molds still rely on extra surface treatments employing release agents to achieve defects-free demolding and meanwhile, mitigate the residual contamination. To address these issues, lubricants-retaining Ni mold was achieved by doping low surface tension polytetrafluoroethylene (PTFE) nano-fillers into the Ni matrix via electrodeposition. The introduction of surfactant mixtures facilitated the successful incorporation of PTFE into the Ni matrix, causing them to perfectly integrate and form as a whole. Such mold exhibited excellent mechanical performance with the enhanced hardness of 452 Hv (2.3-fold increase), low surface roughness of 23 nm in Sa and low surface energy of 28.1 mJ/m2 (33.6% decrease), resulting in a maximum reduction of 28.6% in demolding force. This Ni-PTFE mold can withstand more than 1500 demolding cycles without the need for additional demolding agents or the removal of residual contaminants. Importantly, no PTFE nanoparticles were detected on the produced COC chips, as confirmed by energy dispersive X-ray spectroscopy analysis and Raman spectroscopy, confirming no contamination to the polymer and no lubrication degradation of such mold. Polymer chips produced from such mold displayed well-defined structures and excellent biocompatibility, rendering them suitable for microfluidic applications. Finally, this facile and cost-effective method enables creating a reusable, high-resolution mold with low surface energy, ensuring defects-free demolding for the mass production of polymer parts.
DIAGNÓSTICO DOS SERVIÇOS PRESTADOS PELA MECÂNICA CANAÃ
Authors: Oliveira Patrícia Fonseca , Santos Marcos Ferreira 
Published: October 2024

O ambiente de serviços está cada vez mais tomando espaço na economia. O setor automobilístico em especial tem sido enfatizado em estudos de aprimoramento do processo de serviços, uma vez que os consumidores tornaram-se exigentes, devido às influências do meio de comunicação e a conscientização da necessidade de melhoria dos serviços. Conquistar e manter clientes têm sido o foco das empresas que desejam manter-se no mercado, almejando cada vez mais aumentar o índice de satisfação de seus consumidores, com o intuito de torná-los fiéis e assim garantir a sustentabilidade da empresa, gerada pelo índice de vendas. Com a concorrência isso torna-se mais difícil, é necessário buscar alternativas para se diferenciar e conquistar o poder de compra do consumidor. Em virtude disso, realizou-se um estudo para diagnosticar os serviços prestados pela Mecânica Canaã. Primeiramente descreve-se o problema de pesquisa, que busca diagnosticar os serviços prestados pela Mecânica Canaã, objeto do estudo. Posteriormente, são definidos os objetivos do presente estudo que contribuíram para responder ao problema de pesquisa. O embasamento teórico explica diversos aspectos do marketing de serviços, e como o mesmo contribui para melhoria na prestação do serviço. Logo após são analisados os meios pelos quais foi realizada a pesquisa em campo, os métodos utilizados, seguindo a análise dos resultados, onde são demonstrados os resultados colhidos dos questionários aplicados aos clientes, bem como a entrevista feita aos administradores do estabelecimento. As propostas de melhoria para os gargalos encontrados no diagnóstico são apresentadas nas considerações finais, com alternativas para minimizar o impacto dos pontos negativos no processo de serviço.
O EXÉRCITO NA HISTÓRIA POLÍTICA DO BRASIL: CONSIDERAÇÕES ACERCA DA RACIONALIDADE MILITAR COMO ELEMENTO MOTIVADOR DO GOLPE DE 1964
Authors: Oliveira Ueber José de 
Published: October 2024

Analisa-se, neste artigo, o papel do exército na história política do Brasil, tendo como base a racionalidade militar como elemento motivador do golpe de 1964, este período é um dos mais importantes episódios da história política brasileira enquanto um país independente. E o que mais chama a atenção é o fato de neles despontarem como protagonistas do processo as Forças Armas, evidenciando um componente estrutural decisivo da política brasileira em sua trajetória, qual seja: o poder militar Segundo Maranhão (2004), no Brasil, "[...] o Exército [em especial], principalmente nos seus escalões de chefia, é um importante centro de ação e decisão política interna [..]".
Integrated Exploration of Pyranocoumarin Derivatives as Synergistic Inhibitors of Dual-target for Mpro and PLpro Proteins of SARS-CoV-2 through Molecular Docking, ADMET Analysis, and Molecular Dynamics Simulation
Authors: Yamari Imane , Bouamri Lamiae El , Abchir Oussama , Bouachrine Mohammed , Kouali Mhammed El , Samadi Abdelouahid , Chtita Samir 
Published: October 2024

Aims:
This study aimed to explore the potential of natural anticoagulant compounds
as synergistic inhibitors of the main protease (Mpro) and papain-like protease
(PLpro) of SARS-CoV-2 and find effective therapies against SARS-CoV-2 by investigating
the inhibitory effects of natural anticoagulant compounds on key viral proteases.

Objective:
The objectives of this study were to conduct rigorous virtual screening and
molecular docking analyses to evaluate the binding affinities and interactions of selected
anticoagulant compounds with Mpro and PLpro, to assess the pharmacokinetic and pharmacodynamic
profiles of the compounds to determine their viability for therapeutic use,
and to employ molecular dynamics simulations to understand the stability of the identified
compounds over time.

Methods:
In this study, a curated collection of natural anticoagulant compounds was conducted.
Virtual screening and molecular docking analyses were performed to assess binding
affinities and interactions with Mpro and PLpro. Furthermore, pharmacokinetic and
pharmacodynamic analyses were carried out to evaluate absorption, distribution,
metabolism, and excretion profiles. Molecular dynamics simulations were performed to
elucidate compound stability.

Results:
Natural compounds exhibiting significant inhibitory activity against Mpro and
PLpro were identified. A dual-target approach was established as a promising strategy
for attenuating viral replication and addressing coagulopathic complications associated
with SARS-CoV-2 infection.

Conclusion:
The study lays a solid foundation for experimental validation and optimization
of identified compounds, potentially leading to the development of precise treatments
for SARS-CoV-2.
Quality by Design Approach for the Development of Cariprazine Hydrochloride Loaded Lipid-Based Formulation for Brain Delivery via Intranasal Route
Authors: Chiprikar Pallavi , Mastiholimath Vinayak , Biradar Prakash , Shirkoli Nisha 
Published: October 2024

Background:
Cariprazine (CPZ) is a third-generation antipsychotic medication that has been approved
for treating schizophrenia. This study aimed to develop a cariprazine-loaded nanostructured lipid carrier
(CPZ-NLCs) to prevent first-pass metabolism and improve bioavailability and site-specific delivery from
nose to the brain.

Methods:
The CPZ-NLCs were prepared using melt emulsification. The formulation was optimized using the
Box–Behnken design (BBD); where the influence of independent variables on critical quality attributes, such
as particle size and entrapment efficiency was studied.

Results:
The optimized batch (F6) had a particle size of 173.3 ± 0.6 nm and an entrapment efficiency of 96.1
± 0.57%, respectively. The in vitro release showed >96% release of CPZ from NLC within 30 min. The optimized
formulation's ex vivo studies revealed significantly increased CPZ permeability (>75%) in sheep nasal
mucosa compared to the CPZ suspension (~26%). The ciliotoxicity study of the nasal mucosa revealed that
the CPZ-NLC formulation did not affect the nasal epithelium. The intranasal administration of the formulation
achieved 76.14±6.23 μg/ml concentration in the brain which was significantly higher than the oral CPZ suspension
administration (30.46±7.24 μg/ml). The developed formulation was stable for 3 months.

Conclusion:
The study concluded that the developed CPZ-NLC could significantly improve the bioavailability
with quick delivery to the brain.
Assessing the Effectiveness of an mHealth Intervention to Support Men Who Have Sex With Men Engaging in Chemsex (Budd): Single-Case and Pre-Post Experimental Design Study
Authors: Herrijgers Corinne , Verboon Peter , Florence Eric , Vandebosch Heidi , Poels Karolien , Platteau Tom 
Published: October 2024

Background
This study focuses on the Budd app, a mobile health intervention designed for gay, bisexual, and other men who have sex with men who participate in chemsex. Chemsex, the use of psychoactive drugs in a sexual context, presents substantial health risks including increased HIV transmission and mental health issues. Addressing these risks requires innovative interventions tailored to the unique needs of this population.

Objective
This study aims to evaluate the effectiveness of the Budd app in promoting drug harm reduction practices among its users, focusing on knowledge, behavioral intention, risk behavior awareness, and self-efficacy.

Methods
The study used a mixed methods approach, combining a single-case experimental design and a pre-post study. A total of 10 participants from an outpatient clinic were recruited, and each attended the clinic 3 times. During the first visit, participants installed a restricted version of the Budd app, which allowed them to report daily mood and risk behavior after chemsex sessions. Phase A (baseline) lasted at least 2 weeks depending on chemsex participation. In the second visit, participants gained full access to the Budd app, initiating phase B (intervention). Phase B lasted at least 6 weeks, depending on chemsex participation, with identical data input as phase A. Participants completed pre- and postintervention surveys assessing behavioral determinants during the first and third visit.

Results
The study observed an increased knowledge about chemsex substances postintervention, with a mean percentage improvement in knowledge scores of 20.59% (SD 13.3%) among participants. Behavioral intention and self-efficacy showed mixed results, with some participants improving while others experienced a decrease. There was also a variable impact on awareness of risk behavior, with half of the participants reporting a decrease postintervention. Despite these mixed results, the app was generally well-received, with participants engaging with the app’s features an average of 50 times during the study.

Conclusions
The Budd app showed effectiveness in enhancing knowledge about chemsex substances among gay, bisexual, and other men who have sex with men. However, its impact on safe dosing behavior, behavioral intention, self-efficacy, and risk behavior awareness was inconsistent. These findings suggest that while educational interventions can increase knowledge, translating this into behavioral change is more complex and may require more participants, a longer follow-up period, and additional strategies and support mechanisms.
Integrating Patient-Generated Digital Data Into Mental Health Therapy: Mixed Methods Analysis of User Experience
Authors: Southwick Lauren , Sharma Meghana , Rai Sunny , Beidas Rinad S , Mandell David S , Asch David A , Curtis Brenda , Guntuku Sharath Chandra , Merchant Raina M 
Published: October 2024

Abstract

Background
Therapists and their patients increasingly discuss digital data from social media, smartphone sensors, and other online engagements within the context of psychotherapy.

Objective
We examined patients’ and mental health therapists’ experiences and perceptions following a randomized controlled trial in which they both received regular summaries of patients’ digital data (eg, dashboard) to review and discuss in session. The dashboard included data that patients consented to share from their social media posts, phone usage, and online searches.

Methods
Following the randomized controlled trial, patient (n=56) and therapist (n=44) participants completed a debriefing survey after their study completion (from December 2021 to January 2022). Participants were asked about their experience receiving a digital data dashboard in psychotherapy via closed- and open-ended questions. We calculated descriptive statistics for closed-ended questions and conducted qualitative coding via NVivo (version 10; Lumivero) and natural language processing using the machine learning tool latent Dirichlet allocation to analyze open-ended questions.

Results
Of 100 participants, nearly half (n=48, 49%) described their experience with the dashboard as “positive,” while the other half noted a “neutral” experience. Responses to the open-ended questions resulted in three thematic areas (nine subcategories): (1) dashboard experience (positive, neutral or negative, and comfortable); (2) perception of the dashboard’s impact on enhancing therapy (accountability, increased awareness over time, and objectivity); and (3) dashboard refinements (additional sources, tailored content, and privacy).

Conclusions
Patients reported that receiving their digital data helped them stay “accountable,” while therapists indicated that the dashboard helped “tailor treatment plans.” Patient and therapist surveys provided important feedback on their experience regularly discussing dashboards in psychotherapy.
Pocket de Medicina Familiar: diseño y desarrollo
Authors: Rivero-López Carlos A. , Núñez-Galván Lizbeth A. , López-Ortiz Geovani 
Published: October 2024

La práctica de la medicina familiar requiere un acceso eficiente a información médica actualizada y fiable. El libro Pocket de Medicina Familiar fue desarrollado como un recurso integral para abordar esta necesidad, proporciona a los médicos familiares y otros profesionales de la salud, pautas sobre el manejo de diversas condiciones clínicas de mayor prevalencia en el primer nivel de atención. El diseño y desarrollo de la información se desarrolló a través de 7 pasos: 1. Relevancia, 2. Accesibilidad, 3. Contenido, 4. Dinamismo, 5. Trabajo en equipo, 6. Seguimiento y 7. Evaluación. Cada capítulo del libro fue estructurado y diseñado para ser conciso y accesible en entornos clínicos, e incluyó la definición de la enfermedad, epidemiología, prevención, diagnóstico, manejo, así como aspectos biopsicosociales de relevancia en el ámbito de la medicina familiar.
Обоснование необходимости и направлений совершенствования стратегического планирования инновационного развития предприятий инвестиционно-строительной сферы
Authors: Власенко Вячеслав Александрович 
Published: October 2024

В современных условиях развития инвестиционно-строительной сферы, характеризующихся необходимостью реализации политики технологического суверенитета, импортозамещения и создания собственных конкурентоспособных производств предприятиями, возникает необходимость совершенствования концептуальных и методологических основ активизации инновационного развития строительных предприятий. В статье выявлены особенности инновационной деятельности предприятий инвестиционно-строительной сферы, систематизированы научные подходы к построению методологии стратегического планирования инновационного развития, предложена методика анализа динамики характеристик и выявления трендов инновационного развития в строительстве. На основе проведенного анализа определены основные проблемы и тренды развития пространства инновационной деятельности в отрасли, предложены методы решения проблем и соответствующий инструментарий и направления их научного обоснования. При определении стратегических ориентиров развития строительных организаций, равно как и при определении стратегий развития мезоуровня, соответственно, должен учитываться прежде всего ценностно-ориентированный подход в проекции инновации ценности как устойчивого конкурентного преимущества предприятий, отраслей и регионов, а также стейкхолдерский подход в проекции формирования инновационной сети. Предложен новый подход к планированию и реализации стратегии инновационного развития строительного предприятия, включающий изменение инновационной парадигмы на основе инновации ценности и форсайт-прогноза строительной отрасли. Применение авторского подхода позволит повысить уровень инновационной активности в строительстве и обеспечить устойчивый рост инновационных конкурентных преимуществ предприятия в соответствии с целевыми ориентирами научно-технического и отраслевого развития. Показана целесо­образность совершенствования методологии формирования стратегии инновационного развития предприятий инвестиционно-строительной сферы на основании новой парадигмы инновации ценности, разработана логическая схема совершенствования методологии, позволяющая обеспечить опережающее развитие отечественных строительных предприятий на инновационной основе.
Содержание и итоги производственной практики студентов НИУ МГСУ в республике Вьетнам в 2024 году
Authors: Янковский Александр Владимирович , Хромых Виктория Денисовна , Пономарева Анастасия Александровна , Шокина Яна Ильинична , Чернышова Ангелина Станиславовна 
Published: October 2024

В статье представлена информация о том, как кафедрой Организации строительства и управления недвижимостью (ОСУН) НИУ МГСУ была организована международная практика во Вьетнаме в контексте развития международного взаимодействия. Партнером выступили Хошиминский архитектурный университет и строительная компания Coteccons. НИУ МГСУ и Хошиминский архитектурный университет активно участвуют в совместных научно-образовательных мероприятиях и конференциях, которые проводятся как в России, так и во Вьетнаме. Coteccons является одной из самых уважаемых частных строительных компаний во Вьетнаме, которая была основана в 2004 году. Цель компании: стать инженерно-строительной группой мирового класса, ведущей корпоративной группой во Вьетнаме в XXI веке. Итоги прошедшей производственной практики подвели в Хошиминском архитектурном университете с участием и.о. генконсула РФ консула-советника Ю. Немцова и атташе российского посольства В. Скосырева. Участие в международной практике позволяет студентам получить реальный опыт работы в международной среде, познакомиться с современными технологиями и методами строительства. Работа во Вьетнаме позволила погрузиться в уникальную культуру и традиции страны, а также улучшить профессиональные навыки студентов и сделать их более конкурентоспособными на рынке труда.