bluuebunny/crossref_metadata_2025_split huggingface dataset:
Missing-in-metastasis protein downregulates CXCR4 by promoting ubiquitylation and interaction with small Rab GTPases
Authors: Li Lushen , Baxter Shaneen S. , Gu Ning , Ji Min , Zhan Xi 
Published: March 2017

ABSTRACT
Surface expression of chemokine receptor CXCR4 is downregulated by missing-in-metastasis protein (MIM; also known as MTSS1), a member of the inverse BAR (I-BAR)-domain protein family that recognizes and generates membranes with negative curvature. Yet, the mechanism for the regulation is unknown. Here, we show that MIM forms a complex with CXCR4 by binding to E3 ubiquitin ligase AIP4 (also known as ITCH) in response to stromal cell-derived factor 1 (SDF-1; also known as CXCL12). Overexpression of MIM promoted CXCR4 ubiquitylation, inhibited cellular response to SDF-1, caused accumulation and aggregation of multivesicular bodies (MVBs) in the cytoplasm, and promoted CXCR4 sorting into MVBs in a manner depending on binding to AIP4. In response to SDF-1, MIM also bound transiently to the small GTPase Rab5 at 5 min and to Rab7 at 30 min. Binding to Rab7 requires an N-terminal coiled-coil motif, deletion of which abolished MIM-mediated MVB formation and CXCR4 internalization. Our results unveil a previously unknown property of MIM that establishes the linkage of protein ubiquitylation with Rab-guided trafficking of CXCR4 in endocytic vesicles.
‘Why are there always three?’: The Gothic occult in Dario Argento’s Three Mothers Trilogy
Authors: Hallam Lindsay 
Published: March 2017

Abstract
This article examines the occult and esoteric sources of Dario Argento’s Three Mothers Trilogy, comprising the films Suspiria (1977), Inferno (1980) and La terza madre (The Mother of Tears) (2007). The unique mythology created for these films does not rely on traditional Christian images of evil but instead draws from the practices and history of western esotericism, primarily that of alchemy. The Three Mothers and the powers they wield are expressed through the fundamental elements of the cinematic form, such as light, colour, sound and celluloid, rather than through the surface narrative. Although the films follow Gothic conventions, wherein witchcraft and the occult become synonymous with evil, I will argue that it is within the mise-en-scéne of the films themselves that one can discover the connections to alternative occult traditions.
The cultural capital of the gothic horror adaptation: The case of Dario Argento’s The Phantom of the Opera and Dracula 3D
Authors: Aldana Reyes Xavier 
Published: March 2017

Abstract
Dario Argento is the best-known living Italian horror director, but despite this his career is perceived to be at an all-time low. I propose that the nadir of Argento’s filmography coincides, in part, with his embrace of the gothic adaptation and that at least two of his late films, The Phantom of the Opera (1998) and Dracula 3D (2012), are born out of the tensions between his desire to achieve auteur status by choosing respectable and literary sources as his primary material and the bloody and excessive nature of the product that he has come to be known for. My contention is that to understand the role that these films play within the director’s oeuvre, as well as their negative reception among critics, it is crucial to consider how they negotiate the dichotomy between the positive critical discourse currently surrounding gothic cinema and the negative one applied to visceral horror.
Tauberian theorems for statistically (C,1,1) summable double sequences of fuzzy numbers
Authors: Önder Zerrin , Çanak İbrahim , Totur Ümit 
Published: March 2017

Abstract
In this paper, we prove that a bounded double sequence of fuzzy numbers which is statistically convergent is also statistically (C, 1, 1) summable to the same number. We construct an example that the converse of this statement is not true in general. We obtain that the statistically (C, 1, 1) summable double sequence of fuzzy numbers is convergent and statistically convergent to the same number under the slowly oscillating and statistically slowly oscillating conditions in certain senses, respectively.
A Novel Focal Seizure Pattern Generated in Superficial Layers of the Olfactory Cortex
Authors: Uva Laura , Saccucci Stefania , Chikhladze Maia , Tassi Laura , Gnatkovsky Vadym , Milesi Gloria , Morbin Michela , de Curtis Marco 
Published: March 2017

Seizure patterns identified in focal epilepsies caused by diverse etiologies are likely due to different pathogenic mechanisms. We describe here a novel, region-specific focal seizure pattern that mimics seizure activity observed in a subpopulation of patients submitted to presurgical monitoring with intracerebral electrodes. Distinctive seizure-like events (SLEs) are induced in the olfactory regions by acute treatment of both tangential brain slices and the isolated guinea pig brain with the potassium channel blocker 4-aminopyridine. Analysis of field potentials, intracellular activities, and extracellular potassium changes demonstrates that SLEs in the piriform cortex initiate in the superficial layer 1 lacking principal neurons with an activity-dependent increase of extracellular potassium. SLE progression (but not onset) does not require the participation of synaptic transmission and is mediated by diffusion of potassium to deep cortical layers. The novel seizure pattern here described is not observed in other cortical regions; it is proposed to rely on the peculiar organization of the superficial piriform cortex layers, which are characterized by unmyelinated axons and perisynaptic astroglial envelopes. This study reveals a sequence of ictogenic events in the olfactory cortex that were never described before in other cortical structures and supports the notion that altered potassium homeostasis and unmyelinated fibers may represent a potential vehicle for focal ictogenesis.SIGNIFICANCE STATEMENTWe describe a novel seizure pattern peculiar of the olfactory cortex that resembles focal seizures with low-voltage fast activity at onset observed in humans. The findings suggest that network mechanisms responsible for seizure onset can be region specific.
Deletion of the Fractalkine Receptor, CX3CR1, Improves Endogenous Repair, Axon Sprouting, and Synaptogenesis after Spinal Cord Injury in Mice
Authors: Freria Camila M. , Hall Jodie C.E. , Wei Ping , Guan Zhen , McTigue Dana M. , Popovich Phillip G. 
Published: March 2017

Impaired signaling via CX3CR1, the fractalkine receptor, promotes recovery after traumatic spinal contusion injury in mice, a benefit achieved in part by reducing macrophage-mediated injury at the lesion epicenter. Here, we tested the hypothesis that CX3CR1-dependent changes in microglia and macrophage functions also will enhance neuroplasticity, at and several segments below the injury epicenter. New data show that in the presence of inflammatory stimuli, CX3CR1-deficient (CX3CR1−/−) microglia and macrophages adopt a reparative phenotype and increase expression of genes that encode neurotrophic and gliogenic proteins. At the lesion epicenter (mid-thoracic spinal cord), the microenvironment created by CX3CR1−/−microglia/macrophages enhances NG2 cell responses, axon sparing, and sprouting of serotonergic axons. In lumbar spinal cord, inflammatory signaling is reduced in CX3CR1−/−microglia. This is associated with reduced dendritic pathology and improved axonal and synaptic plasticity on ventral horn motor neurons. Together, these data indicate that CX3CR1, a microglia-specific chemokine receptor, is a novel therapeutic target for enhancing neuroplasticity and recovery after SCI. Interventions that specifically target CX3CR1 could reduce the adverse effects of inflammation and augment activity-dependent plasticity and restoration of function. Indeed, limiting CX3CR1-dependent signaling could improve rehabilitation and spinal learning.SIGNIFICANCE STATEMENTPublished data show that genetic deletion of CX3CR1, a microglia-specific chemokine receptor, promotes recovery after traumatic spinal cord injury in mice, a benefit achieved in part by reducing macrophage-mediated injury at the lesion epicenter. Data in the current manuscript indicate that CX3CR1 deletion changes microglia and macrophage function, creating a tissue microenvironment that enhances endogenous repair and indices of neuroplasticity, at and several segments below the injury epicenter. Interventions that specifically target CX3CR1 might be used in the future to reduce the adverse effects of intraspinal inflammation and augment activity-dependent plasticity (e.g., rehabilitation) and restoration of function.
Molecular Analysis of Vascular Endothelial Growth Factor (VEGF) Receptors in EUS-guided Samples Obtained from Patients with Pancreatic Adenocarcinoma*
Authors: Costache Mădălin Ionuț , Iordache Sevastița , Costache Cornelia Alexandra , Dragos Ene , Dragos Alexandru , Săftoiu Adrian 
Published: March 2017

Background & Aims: Vascular endothelial growth factor (VEGF) and its receptors (VEGF-R1 and VEGF-R2) are the most important angiogenesis stimulating factors in pancreatic cancer. This study aims to assess VEGF-R1 and VEGF-R2 gene expression in EUS-FNA samples and identify prognostic markers in pancreatic adenocarcinoma.Methods. This was a retrospective study of prospectively collected data of 88 consecutive patients, with clinical and imaging suspicion of pancreatic neoplasms, based on samples obtained through endoscopic ultrasound-guided fine needle aspiration (EUS-FNA).Results. EUS had an accuracy of 93.2% for the diagnosis of pancreatic cancer. Based on real-time qPCR analysis, VEGF-R1 and VEGF-R2 expressions were present in 90% and 65% of the analysed malignant samples, respectively; 89% of the patients died during the study, with a median survival rate of only 9 months. The survival was correlated with the initial stage and with the presence of VEGF-R1 and VEGF-R2 gene expression. We found that there are significant correlations between death/survival and T stage, N stage, resectability status, VEGF-R1, VEGF-R2 and VEGF-R1/VEGF-R2 coexpression. Using a Cox model regression our study demonstrates that VEGF-R1/VEGF-R2 coexpression might be considered as a poor prognostic factor in pancreatic cancer.Conclusions. EUS is a very effective technique for the diagnosis and staging of pancreatic adenocarcinoma in patients with clinical and imaging suspicion of pancreatic neoplasm, with an accuracy of 93.2%. Furthermore, the role of molecular analysis of EUS-guided FNA samples was established by the assessment of VEGF-R1, VEGF-R2 gene expression, which might be considered prognostic markers in pancreatic cancer.Abbreviations: cDNA: deoxyribonucleic acid concentration; CE-EUS: contrast enhanced endoscopic ultrasound; CT: computed tomography; EUS: endoscopic ultrasound; EUS–FNA: endoscopic ultrasound guided fine needle aspiration; IHC: immunohistochemistry; MRI: magnetic resonance imaging; qRT–PCR: quantitative real time polymerase chain reaction; RNA: ribonucleic acid; VEGF: vascular endothelial growth factor; VEGF-R1: vascular endothelial growth factor receptor 1; VEGF-R2: vascular endothelial growth factor receptor 2.
G Protein-Coupled Receptor 30 (GPR30) Expression Pattern in Inflammatory Bowel Disease Patients Suggests its Key Role in the Inflammatory Process. A Preliminary Study
Authors: Włodarczyk Marcin , Włodarczyk Aleksandra Sobolewska , Cygankiewicz Adam I. , Jacenik Damian , Polańczyk Aleksandra Piechota , Michalska Krystyna Stec 
Published: March 2017

Background & Aims: G protein-coupled receptor 30 (GPR30) is a recently de-orphanized estrogen receptor that mediates the effects of estrogens on different cells. It has been postulated that in inflammatory bowel diseases (IBD) activation of GPR30 blocks the pathways dependent on pro-inflammatory cytokines. The aim of our study was to investigate GPR30 expression in patients with IBD and its potential implication in future therapies.Methods. Fifty-seven patients were enrolled in our study: 20 subjects with Crohn’s disease (CD), 22 with ulcerative colitis (UC) and 15 controls. In each subject, biopsies were taken from various left-colonic locations. Gene and protein expression of GPR30 was quantified using real time RT-PCR or Western blot.Results: GPR30 mRNA and protein expression were detected in all tested colonic tissues. No significant differences in GPR30 gene expression were observed. In non-inflamed areas, GPR30 protein was strongly increased in CD patients, but moderately in UC patients (p= 0.014 and p=0.143, respectively, vs. controls). In CD patients, a significantly lower GPR30 protein content in inflamed than in non-inflamed tissue was observed (p=0.039). The change was independent of patient gender.Conclusion: Our observations indicate that GPR30 may play a role in the development and progression of inflammatory lesions in IBD, thus affecting disease severity, and consequently IBD treatment. Therefore, GPR30 may become an attractive target for novel anti-IBD drugs, particularly in CD.Abbreviations: CRP: C-reactive protein; C-IBS: constipation-predominant IBS; CD: Crohn’s disease; D-IBS: diarrhea-predominant IBS; ER: Estrogen; GPER: G protein – coupled estrogen receptor 1; GPR30: G protein-coupled receptor 30; GI: gastrointestinal; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IBD: inflammatory bowel diseases; IL: Interleukin; ICAM-1: intracellular cell adhesion molecule-1; IBS: irritable bowel syndrome; PDL: periodontal ligament; Th: T helper; TNF-α: tumor necrosis factor-α; UC: ulcerative colitis; VCAM-1: vascular cell adhesion molecule-1; WBC: white blood cells.
Antiproliferative and Apoptotic Effects of Lidocaine on Human Hepatocarcinoma Cells. A preliminary study
Authors: Jurj Ancuta , Tomuleasa Ciprian , Tat Tiberiu T. , Neagoe Ioana Berindan , Vesa Stefan V. , Ionescu Daniela C. 
Published: March 2017

Background & Aims: It is now well documented that certain anesthetic techniques may influence long term outcome in cancer patients undergoing surgery. More recently, local anesthetics proved certain antiproliferative effects in cancer cells. In our study, we aimed to investigate if lidocaine has antiproliferative effects in human hepatocarcinoma cells and to identify possible mechanisms of these effects.Methods. We investigated the inhibitory effect of different concentrations of lidocaine on the proliferation of cultured HepG2 human hepatocarcinoma cells and LX2 normal liver fibroblasts. Cells were exposed to nine different concentrations of lidocaine for 72h. MTT assay was used to investigate HepG2 and LX2 proliferation while Western blotting was used for detection of p53 expression level.Results. Our data showed that lidocaine inhibited cell proliferation in a concentration-dependent manner in both HepG2 and LX2. The antiproliferative effects of lidocaine in LX2 were significantly diminished as compared with those in HepG2 (p< 0.001). Similarly, the expression level of p53 was significant decreased in HepG2 lines treated with lidocaine as compared with control and LX2 (p = 0.0241).Conclusions. In clinically relevant concentrations, lidocaine had significant antiproliferative effects on human hepatocarcinoma cells. These effects were time and dose-dependent. One of the possible mechanisms of these effects is by modifying the P53 expression level. The relevance of these findings in clinical practice is limited; clinical impact of these effects on the outcome of patients with hepatocarcinoma undergoing surgery or minimal invasive procedures needs to be demonstrated in future animal models and clinical studies.Abbreviations: DMSO: dimethyl sulfoxide; DNA: deoxyribonucleic acid; EDTA: ethylenediaminetetraacetic acid; EGFR: epidermal growth factor receptor; MTT assay: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay; NK cells: natural killer cells; RCTs: randomized controlled studies; VGSC: voltage gated sodium channels.
LE TERME « ÉCOTOURISME » ET SES QUASISYNONYMES DANS L’ESPACE MÉDIATIQUE FRANÇAIS
Authors: MARTINEZ Christine 
Published: March 2017

RÉSUMÉ. L’évolution du lexème écotourisme dans les quotidiens français (version informatisée) est le but de notre étude. Nous nous référons à Moirand (2007, p. 4) pour voir ce qui se passe « dans et par les médias » et à Charaudeau et Maingueneau (2002, p. 324) pour la mémoire des discours. Non seulement l’usage et les conditions sociales sont importants dans l’appréciation, la compréhension et la manière, dont sont présentées nos collocations, mais surtout le contexte dans lequel elles évoluent. Qu’il soit écologique, officiel (Ministère de l’Ecologie, du Développement durable et de l’Énergie) ou journalistique (slogan de propagande écologique), ces contextes nous paraissent essentiels pour une bonne compréhension. Notre but est l’analyse de ce phénomène. Le concept de écotourisme est riche en traits sémantiques, et malléable suivant les contextes et les visées médiatiques qu’on lui impose. Nous allons essayer d’approfondir ces singularités dans cet article. Mots-clés : collocation, contexte, discours, écotourisme, journaliste, manipulation, trait sémantique, visée discursive. ABSTRACT. The evolution of the lexeme ecotourism in French newspapers (electronic version) is the aim of our study. We refer to Moirand (2007, p. 4) to see what happens "in and through the media" and Patrick Charaudeau et Maingueneau (2002, p. 324) for the memory of the speech. Not only the use and social conditions are important in the appreciation, understanding and speaking, which are presented our collocations, but especially the context in which they operate. It is environmentally friendly, official (Ministry of Ecology, Sustainable Development and Energy) or journalistic (ecological propaganda slogan), these contexts we consider essential for a proper understanding. Our ambition is to analyse this phenomenon. The concept of ecotourism is rich in semantic traits and malleable depending on the context and media referred imposed on it. We will try to deepen these singularities in this article. Keywords: collocation, context, discourse, ecotourism, journalist, manipulation, semantic trait, discursive aim.
FIBROMA OSSIFICANTE CENTRAL EM MANDÍBULA: RELATO DE CASO
Authors: Lima Junior Francisco de Assis Almeida , Cunha John Lennon Silva , Rosa Candice Rebouças , Fonte Juliana Batista Melo , Melo Maria de Fátima Batista , Albuquerque Junior Ricardo Luiz Cavalcanti 
Published: March 2017

O Fibroma Ossificante Central (FOC) é uma neoplasia benigna de origem fibro-óssea incomum que acomete os ossos da face, caracterizado pela substituição da arquitetura óssea normal por tecido conjuntivo rico em fibroblastos e fibras colágenas, com deposição de material mineralizado em quantidade e morfologia variada. Estudos tem demonstrado a existência de muita superposição entre os aspectos clínicos, imaginológicos e histopatológicos das lesões fibro-ósseas benignas dos maxilares, determinando dificuldades no estabelecimento de um diagnóstico seguro. O objetivo deste trabalho foi relatar um caso de FOC em uma localização incomum, com ênfase na discussão de sua etiopatogênese e critérios de diagnóstico diferencial imaginológico e histopatológico entre esta entidade e a outras lesões fibro-ósseas dos maxilares, como a displasia óssea fibrosa e o fibroma ossificante juvenil agressivo. A análise do caso e sua relação com a literatura vigente permitiu concluir que é de extrema relevância que o diagnóstico do FOC seja devidamente embasado numa correlação de achados clinicopatológicos para assegurar o estabelecimento do diagnóstico e terapêutica mais apropriados.
Penggabungan, Peleburan dan Pengambilalihan pada Industri Telekomunikasi di Indonesia
Authors: Maharani Diah Arum , Wulandari Helena Wirastri 
Published: March 2017

<p align="justify"><strong>Abstrak</strong> Kajian tentang penggabungan, peleburan dan pengambilalihan dari penyelenggara (operator) telekomunikasi seluler di Indonesia dilakukan untuk lebih menciptakan iklim yang sehat dan membangun perekonomian nasional tanpa merugikan pemain di sektor ini dan juga konsumen. Evaluasi penggabungan, peleburan, dan pengambilalihan dilakukan oleh masing-masing instansi terkait sesuai dengan kewenangan yang diberikan oleh Undang-Undang. Koordinasi tata cara/prosedur penggabungan, peleburan, dan pengambilalihan antara instansi terkait perlu dilakukan. Beberapa hal yang disarankan perlu dilakukan oleh Kemkominfo/BRTI terhadap penggabungan, peleburan, dan pengambilalihan penyelenggara telekomunikasi seluler diantaranya penilaian pre-merger dan pengawasan post-merger. Penilaian pre-merger melalui nilai perusahaan dan penilaian kelayakan (pre-merger) dalam hal strategic and business due diligence (kecuali isu hukum persaingan usaha); technological &amp; integration issues; financial &amp; commercial due diligence (kecuali isu hukum perusahaan); dan public interest. Sementara itu, pengawasan post-merger meliputi: laporan berkala tentang pencapaian komitmen, laporan berkala tentang kinerja, dan pengawasan terhadap kewajiban interkoneksi.</p><p align="justify"> </p><p align="justify"><strong>Abstract</strong> Studies on merger, consolidation and acquisition of mobile telecommunications providers(operators) in Indonesia is to be carried out to further create a healthy climate, and build the national economy, which would not be detrimental to the players and consumers in the sector. The evaluation of merger, consolidation, and acquisitions were carried out by each of the relevant agencies in accordance with the authority granted by the Act. The coordination of the procedure / merger procedure, consolidation or acquisition between the relevant agencies is to be implemented. Based on this study Kemkominfo / BRTI is recommended to assess the pre-merger and supervision of post-merger through the company's value and feasibility assessment (pre-merger) in terms of strategic and business due diligence (except in the law concerning competitive issues); technological and integration issues; financial &amp; commercial due diligence (except for the company's legal issues); and public interest. The post-merger includes: periodic reports on the achievement of commitments, periodical reports on the performance and supervision of interconnection obligations.</p>