bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of the histone chaperone ASF1B reduces female reproductive capacity in mice
Authors: Messiaen S , Guiard J , Aigueperse C , Fliniaux I , Tourpin S , Barroca V , Allemand I , Fouchet P , Livera G , Vernet M 
Published: February 2016

AbstractAnti-silencing function 1 (ASF1) is an evolutionarily conserved histone H3–H4 chaperone involved in the assembly/disassembly of nucleosome and histone modification. Two paralogous genes,Asf1aandAsf1b, exist in the mouse genome.Asf1ais ubiquitously expressed and its loss causes embryonic lethality. Conversely,Asf1bexpression is more restricted and has been less studied. To determine thein vivofunction ofAsf1b, we generated aAsf1b-deficient mouse line (Asf1bGT(ROSA-βgeo)437) in which expression of thelacZreporter gene is driven by theAsf1bpromoter. Analysis ofβ-galactosidase activity at early embryonic stages indicated a correlation betweenAsf1bexpression and cell differentiation potential. In the gonads of both male and female,Asf1bexpression was specifically detected in the germ cell lineage with a peak expression correlated with meiosis. The viability ofAsf1b-null mice suggests thatAsf1bis dispensable for mouse development. However, these mice showed reduced reproductive capacity compared with wild-type controls. We present evidence that the timing of meiotic entry and the subsequent gonad development are affected more severely inAsf1b-null female mice than in male mice. In female mice, in addition to subfertility related to altered gamete formation, variable defects compromising the development and/or survival of their offspring were also observed. Altogether, our data indicate the importance ofAsf1bexpression at the time of meiotic entry, suggesting that chromatin modifications may play a central role in this process.
A Novel Triage Approach to Identifying Health Concerns
Authors: Bezem Janine , Theunissen Meinou , Kamphuis Mascha , Numans Mattijs E. , Buitendijk Simone E. , Kocken Paul 
Published: February 2016

BACKGROUND:
We investigated the detection of health problems in preventive child health care (PCH) by a novel triage approach for routine health assessments. In the triage approach, all children were preassessed by a physician’s assistant, and only those in need of follow-up were assessed by a PCH physician or nurse. In the traditional approach, all children were assessed by a PCH physician or nurse.

METHODS:
A prospective cohort design was used with data on routine assessments of 1897 children aged 5 to 6 and 10 to 11 years. Primary outcomes were the detection of overweight, visual disorders, and psychosocial problems, with type of approach (traditional vs triage) as independent variable. To assess the severity of health problems, BMI, Snellen, Strengths and Difficulties Questionnaire, and Child Behavior Checklist, scores were compared for both approaches in subgroups of children with overweight, visual disorders, or psychosocial problems.

RESULTS:
No significant differences were found between the approaches in terms of the detection of incident cases of overweight, visual disorders, and psychosocial problems. Significantly higher Strengths and Difficulties Questionnaire scores were found in the subgroup with psychosocial problems when the triage approach was used. Marginal differences between the approaches were found for severity of overweight in the subgroup of overweight children.

CONCLUSIONS:
A novel triage approach to PCH resulting in less involvement of physicians and nurses in routine assessments appears to detect health problems as effectively as the traditional approach in place. More research is needed to determine the long-term outcomes of the 2 approaches.
TEMA 12-2016: Cetoacidosis diabética y estado hiperglicémico hiperosmolar: un enfoque práctico
Authors: Arrollo Sánchez Gloriana , Quirós Cárdenas Saúl 
Published: February 2016

<p>La cetoacidosis diabética (CAD) y elestado hiperglicémicohiperosmolar (EHH), en conjunto conocidas como crisis o emergencias hiperglicémicas, tradicionalmente han sido asociadas a diabetes mellitus mal controlada. Ambas son entidades diferentes desde el punto de vista del cuadro clínico y los hallazgos de laboratorio, no obstante, en ocasiones pueden sufrir un traslape entre ellas. Producto de lo antes dicho,es importante que el personal médico tenga una adecuada comprensión de la fisiopatología, los factores desencadenantes, las manifestaciones clínicas y de laboratorio de dichas condiciones, de manera que se pueda realizar un diagnóstico correcto que conduzca a la instauración de tratamiento temprano y adecuado.</p>
IgA1 Protease Treatment Reverses Mesangial Deposits and Hematuria in a Model of IgA Nephropathy
Authors: Lechner Sebastian M. , Abbad Lilia , Boedec Erwan , Papista Christina , Le Stang Marie-Bénédicte , Moal Christelle , Maillard Julien , Jamin Agnès , Bex-Coudrat Julie , Wang Yong , Li Aiqun , Martini Paolo G.V. , Monteiro Renato C. , Berthelot Laureline 
Published: February 2016

IgA nephropathy (IgAN), characterized by mesangial IgA1 deposits, is a leading cause of renal failure worldwide. IgAN pathogenesis involves circulating hypogalactosylated IgA1 complexed with soluble IgA Fc receptor I (sCD89) and/or anti–hypogalactosylated-IgA1 autoantibodies, but no specific treatment is available for IgAN. The absence of IgA1 and CD89 homologs in the mouse has precluded in vivo proof-of-concept studies of specific therapies targeting IgA1. However, the α1KI‑CD89Tg mouse model of IgAN, which expresses human IgA1 and human CD89, allows in vivo testing of recombinant IgA1 protease (IgA1‑P), a bacterial protein that selectively cleaves human IgA1. Mice injected with IgA1‑P (1–10 mg/kg) had Fc fragments of IgA1 in both serum and urine, associated with a decrease in IgA1–sCD89 complexes. Levels of mesangial IgA1 deposits and the binding partners of these deposits (sCD89, transferrin receptor, and transglutaminase 2) decreased markedly 1 week after treatment, as did the levels of C3 deposition, CD11b+ infiltrating cells, and fibronectin. Antiprotease antibodies did not significantly alter IgA1‑P activity. Moreover, hematuria consistently decreased after treatment. In conclusion, IgA1‑P strongly diminishes human IgA1 mesangial deposits and reduces inflammation, fibrosis, and hematuria in a mouse IgAN model, and therefore may be a plausible treatment for patients with IgAN.
Exposure to English Linguistic Environment, Affective Filters and the Writing Competence of Students
Authors: Natad Joan S. 
Published: February 2016

<p>This descriptive-correlation study aimed to determine the influence of exposure to the English linguistic environment and affective filters on the writing competence of 176 students enrolled in English Proficiency Program (EPP) classes of a college in Davao in the school year 2011-2012. The random sampling technique, three researcher-made questionnaires and 7 statistical tools specifically frequency count, percentage, mean, analysis of variance (ANOVA), t-test, Pearson Product Moment of Correlation or Pearson r and linear regression analysis were used. The study concluded that: the English Proficiency Program (EPP) students have a moderate level of exposure to the English linguistic environment, a low level of affective filters and a very low level of writing competence; there is no significant difference in the level of affective filters and the level of writing competence of students when grouped according to type of high school attended and course; and there is no significant relationship between exposure to the English linguistic environment and writing competence but there is a significant relationship between affective filters and writing competence.</p><p> </p><p><strong>Keywords:</strong> Linguistics, linguistic environment, affective filters, writing competence, descriptive-correlation, Davao City, Philippines.</p>
Selected Paintings of Davaoeño artists: The Perspective of artists and viewers
Authors: Sagarino Emma V. , Baucan Villalyn B. , Habla Rebecca P. 
Published: February 2016

<p>Artwork as a medium of expression reveals themes such as politics, culture, practices, beliefs, environment and many more. The value of artwork may lie on the appreciation given by the viewers. Thus, this study justified whether the youth of today possessed the ability to recognize and understand the messages conveyed through an artwork. Six paintings of two artists from Davao City, Philippines were explored and unveiled. The paintings were shown to 20 purposively chosen students from the University of the Immaculate Conception, Davao City. After the viewing, two separate Focus Group Discussions (FGD) were conducted. The two artists were also interviewed as to the themes and messages they have embodied through their paintings. The common theme conveyed by Engr. Mendros is anchored on Biblical verses. It is understood that his viewers were able to interpret the messages– “The Creation” and Jesus Walking on Top of the Water” because the study participants were composed of Philosophy students who were ex-seminarians. They were able to relate to most of the symbols reflected in the paintings. Mr. Espinosa had revealed Filipino culture and some political issues and many of his viewers were able to express the messages as they were able to relate with them as well.</p><p> </p><p> <strong>Keywords:</strong>Education, artwork, Dabawenyo artists, paintings, Davao City, Philippines</p><div> </div>
Portals of Institutional Culture: University Confession Pages in Focus
Authors: Geonzon Christense , Gerasmio Anna , Lanos Khimberly , Salvan Frevilyn , Tagaytay Monique Musni 
Published: February 2016

One of the innovations in the social media landscape is the proliferation of confession pages on Facebook which allow social media users to “confess” their thoughts, feelings and experiences without revealing their true identity. Confession pages associated with specific higher educational institutions have become a venue for student revelations about everything and anything pertaining to campus life. This multiple case study ventured to find out if the university confession page serves as a peep hole into institutional culture as revealed by the confessions made by users on Facebook and also to figure out whether users’ perception about their own school as well as other schools is affected by the content that they read on university confession pages. The study selected three higher educational institutions in Davao City as its locale. To obtain thick descriptions of this phenomenon, the researchers triangulated between transcripts of conducted focus group discussions, and open- ended questionnaires and content analysis of actual posts from the selected university confession pages. The data revealed that the cloak of anonymity and absence of direct consequence emboldens those who “confess” and serves much like a freedom wall. The study also reveals that while most informants used the confession page because it is the current “in” thing, reading the posts somewhat changed their perception about their school as the hallowed hall of learning as some posts shed light on events and experiences on campus.<div> </div><strong>Keywords</strong>: Communication, Social media, Facebook, confession pages, descriptive qualitative, Philippines
The quality and effect on plant growth of tap water and rainwater in DAVAO CITY
Authors: Limjuco Renan P. , Quinamot Sheila Mae V. , Guy-Ab Jr. Victor L. , Cabañero Cashelmara B. , Tarongoy III Brisbane Peter B. , Bruno Ana Jennine P. , Ybañez Jr. Elvis B. 
Published: February 2016

<p>Rainwater is essential in the sustenance of ecosystem in all parts of the world. It is a free source of nearly pure water and naturally replenishes the earth’s water supply. On the other hand, tap water is one of the most available types of water intended for human consumption while rainwater serves many purposes too. This study aimed to assess the quality of tap water and rainwater in Davao City, specifically in Agdao, Ecoland and Artiaga. The proponents used plant growth of selected varieties such as mongo, radish, tomato, eggplant, and squash, as the main parameter in comparing the rainwater and tap water after 30 days; pH, conductivity, and Total Dissolved Solids (TDS) were also used as additional parameters, which were determined at the Science Resource Center of the University of the Immaculate Conception. This study employed of a descriptive – comparative design making use of quantitative measurements and data from laboratory analyses. The results revealed that rainwater and tap water in Davao city are both safe for plant growth as indicated by the values of the parameters which are within the acceptable range describing a good quality for growing plants.</p><p> </p><p><strong>Keywords:</strong> Plant growth, tap water, rainwater, pH, conductivity, Total Dissolved Solids, Davao City, Philippines</p>
Interval Waktu Penambahan Ampas Mengkudu (Morinda citrifolia L.) dalam Ransum terhadap Performans Ayam Broiler
Authors: Primandini Yuni , Mahfudz Luthfi Djauhari , Sukamto Bambang 
Published: February 2016

Time interval of supplementation of Morinda citrifolia L. by product on the performance broiler chicken ABSTRACT. The research using Completely Randomized Design with 3x2 factorial arrangement and 4 replicated. Dose of Morinda citrifolia by product (T) that is 0 g/kg ( T0); 0.50 g/kg ( T1); 0.75 g/kg ( T2) and 1.00 g/kg (T3) as first factor and time interval that is continue (A1) and 1 week interval (A2) as second factor. The performance parameters observed were : feed intake, body weight and small intestine measure. The result showed that no significant effect of time interval and interaction between time interval with Morinda citrifolia by product dose (p0.05) on feed intake and small intestine measure. The addition dose of Morinda citrifolia by product increased (p0.05) body weight, there was interaction (p0.05) between time interval and Morinda citrifolia by product dose on body weight.
Pengaruh Pupuk Organik Cair NASA terhadap Nitrogen Bintil Akar dan Produksi Macroptilium Atropurpureum
Authors: Husin M. Nur 
Published: February 2016

The effect of liquid organic fertilizing NASA on nitrogen root nodule and production of siratro ABSTRACT. A study was carried out in Experimental Farm and Laboratory for Animal Nutrition of the Faculty of Agriculture in Syiah Kuala University, Darussalam Banda Aceh to know the dose of liquid organic fertilizing (LOF) NASA on nitrogen root nodule and production of Siratro plants. The study use pot (plastic bag) containing 15 kg of soil. The soil type was of kind of Alluvial. Experimental design was of Completely Randomized Design (CRD). Treatment with LOF NASA was of 0.00 cc/l water, 2.50 cc/l water, 5.00 cc/l water, 7.50 cc/l water, and 10 cc/l water. Result showed that a maximum nitrogen root nodule of Siratro 8.54 mg/m2/day and maximum dry matter production of Siratro 2.26 g/m2/day was founded at 10 cc/l water LOF NASA.
Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan
Authors: Dasrul Dasrul , Yaman M. Aman , Zulfan Zulfan 
Published: February 2016

Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purposeABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the spermatozoa quality, sex ratio between X and Y, capacity of fertility indicated by conception rate and sex ratio of goat boar kids. Samples, used in this experiment, are fresh semen from Boer goat with high quality consists of 4 group treatments with 6 replications 1) group of spermatozoa without separation (control), 2) group of spermatozoa separated by percoll gradient density centrifugation 3 levels (P1), 5 levels (P2) and swine up (P3). The observed parameters are spermatozoa quality, X and Y spermatozoa ratio, fertility’s capacity and sex ratio on the birth. Quality examination of spermatozoa and identify X and Y spermatozoa is based on the standard method of WHO. The conception rate was based on the ratio of pregnant goat after the first insemination. Data of spermatozoa quality and spermatozoa ratio were analyzed by using analisis of variance (ANOVA) and further analysis by LSD if there were differences between treatments. The results of this experiment showed that spermatozoa quality Boer goat significantly reduced (p0,05) after separation with percoll gradient density centrifugation and swim up. Percentage spermatozoa X after percoll gradient density centrifugation was significantly higher (P0,05) compared to control and swim up. Meanwhile, the Y spermatozoa population was significantly higher (P0,05) after swim up treatment compared to percoll gradient density centrifugation and control. The percentage of sex ratio (male: female) after insemination from percoll gradient density centrifugation produced more female than male. On the other hand, insemination from swim up produced more male than female. Sex ratio produced from separation of percoll gradient density centrifugation, swim up was difference from control semen (P0,05). From this experiment, it was concluded that spermatozoa separation by percoll gradient density centrifugation and swim up can be used as one of the methods to separate X and Y spermatozoa and further can be applied to get preferred sex animals.
Kajian Level Kadar Air dan Ukuran Partikel Bahan Pakan Terhadap Penampilan Fisik Wafer
Authors: Pujaningsih Retno Iswarin , Prasetiyono Bambang Waluyo Hadi Eko , Mukodiningsih Sri , Tampoebolon Baginda Iskandar Moeda , Utama Cahya Setya 
Published: February 2016

Study on the level of water content and particle size of feed ingredients to the physical appearance of wafer ABSTRACT. This study attempted wafer manufacturing technology development of conventional feed ingredients. The benefit of wafer increase feed consumption and feed efficiency, increase the metabolizable energy content of the feed, kill pathogenic bacteria, reduce the amount of feed scattered, extending the storage time, ensure the balance of feed nutrients and vitamins to prevent oxidation. Research was continuing several research activities on wafer manufacturing technology that has been done and continues to be developed by the researchers. The long term goal of a series of research is to obtain basic information to the wafer manufacturing optimum quality and measurable. Specific target is to obtain basic information about the quality of the wafer on the level of water content and the use of a certain particle size. The research method used was experimental and analytical methods in the laboratory. The results showed that based on the test of physical qualities (moisture content, density, water absorption) showed that the use of the level of moisture content up to 8% with a particle size of 10-20 mm feed material provides the best physical appearance of wafer.
Pengaruh Nisbah Energi-Protein, Nitrogen-Sulfur dan Kalsium-Fosfor Terhadap Produk Metabolisme Rumen dan Kecernaan Substrat
Authors: Suwandyastuti S.N.O. 
Published: February 2016

Influence of the ratio of energy-protein, nitrogen-sulphur, and calsium-phosphor upon rumen metabolism product and digestibility of substratABSTRACT. The Rumen microbes are capable to digest the glucosa polymer of plant waste for energy source and can used the Non Protein Nitrogen (NPN) for body protein synthesis, if the other precussor (Sulphur, Phospor and Branch Chain of Carbon) are available. To know the effectivity of the utilization of plant waste for energy, an experiment have been conducted by in vitro method, used the Randomized Black Design, four replication, factorial 33. The factors tested are : (1) three levels of energy : protein (E/P) ratio : 4, 5 and 6; (2) three levels of Nitrogen : Sulphur (N/S)ratio: 7.5, 10 and 12.5; (3) three levels of Calsium : Phospor (Ca/P) ratio : 0.5, 1 and 2. The variables measured are : synthesis of protein microbes (SPM) ; production of Volatile Fatty Acid (VFA) and Nitrogen Ammonia (N-NH3); the digestibility of substrat. Based on the all variable measured, the experiment can be concluded : (1) the effectivity of the utilization of rice straw will be increased if it used is fortified with 50 percent TDN as energy source (E/P=4), 0.20 percent dry matter of sulphur (N/S=10) and 0.0625 - 0.125 percent dry matter (DM) of phospor (Ca/P=1.0 – 2.0); (2) To stimulate the activity of cellulolytic microbes, its need the fortification of sulphur until reach the optimum level (must be investigated).
Penambahan Tepung Daun Katuk (Saurupus Androgynus L. Merr) dalam Ransum Terhadap Pertambahan Berat Badan dan Lingkar Scrotum Kambing Jantan Peranakan Ettawa
Authors: Yustendi Dedhi , Dasrul Dasrul , Rachmadi Didy 
Published: February 2016

The addition of katuk leaf meal (saurupus androgynus l.merr) in feeds on body weight gain and scrotal circumference male ettawa grade goatsABSTRACT. This research was aimed to study the effect of addition of katuk leaf meal (Sauropus androgynus L. Merr) in feed on body weight gain and scrotal circumference of male Ettawa grade goat. The research lasted for eight months, starting Agustus 2012 to Maret 2013, with feeding trial for two months. Twenty male Ettawa grade goats of 18 to 24 months old, were divided into 4 treatment groups. Treatment groups were added with 0% Katuk leaf meal (R0); 5% (R1); 10% (R2) and 15% (R3), with 5 goats in each replication. Treatment diets were feed in the morning and afternoon for 60 days. Weighing body weight and measuring scrotal circumference was done every week. Data were analyzed using analysis of variance (ANOVA) and if there was significantly different, the data were further tested using Duncan multiple range test. The result shows that the body weight gain of the treatment were, R0 (2,39 ± 0,43 kg); R1 (2,84 ± 0,36 kg); R2 (2,85 ± 0,20 kg) and R3 (3,42 ± 0,78 kg) respectively. Scrotal circumference were, R0 (0,74 ± 0,10 cm); R1 (1,24 ± 0,26 cm); R2 (1,26 ± 0,40 cm) dan R3 (1,32 ± 0,32 cm) respectively. The result of this research indicated that the addition of katuk leaf meal in feed had significant effects (P0,05) on body weight gain and scrotal circumference of Ettawa grade goat.
Analisa Keasaman dan Total Bakteri Asam Laktat Yogurt Akibat Bahan Baku dan Persentase Lactobacillus casei yang Berbeda
Authors: Zakaria Yusdar , Yurliasni Yurliasni , Delima Mira , Diana Ely 
Published: February 2016

Analyze of acidity and amount of lactic acid bacteria of yogurt made from different milk types and lactobacillus casei percentageABSTRACT. The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter to the amount of Lactic Acid Bacteria in yogurt and its pH value and Lactic Acid value. Factorial Completely Randomized Design with two factors and three replications was applied. The A factor was the milk types that were a1 = Powder Milk, a2 = Fresh Milk and a3 = UHT milk. The B factor was the percentages of Lactobacillus casei (b1 = 5% and b2 = 10%). Parameters observed were the level of lactic acid, the pH value of yogurt and the amount of lactic acid bacteria. The result showed significantly difference (P0.01) from the use of milk types and Lactobacillus casei percentages on the lactic acid level, and there are no interaction between factors. There was also significant different (P0.01) showed from pH value and an interaction between both factors was presented. In addition there was significant difference (P0.05) showed by the amount of lactic acid bacteria, and an interaction between both factors was also existed. The research concluded that the higher the percentage of starter, the higher the level of lactic acid and the amount of lactic acid bacteria of yogurt that made from all milk types. On the contrary, the higher the percentage of starter, the lower the pH value of yogurt.