bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐Radiological Method for Fabrication of a Screw‐Channel Drilling Guide in Cement‐Retained Implant Restorations Using Intraoral Digital Scanning and Imaging Superimposition: A Clinical Report
Authors: Mai Hang‐Nga , Kim Kyung‐Rok , Lee Du‐Hyeong 
Published: March 2016

AbstractThe difficulty of retrieving the abutment screw is a major disadvantage of cement‐retained implant restorations. Conventional methods for locating the screw‐access hole are based largely on radiography or manual labor, which limits accuracy and clinical feasibility. This clinical report describes a non‐radiological method for fabricating an accurate drilling guide for location of the screw channel using intraoral optical scanning, 3D superimposition, and computer‐aided design and computer‐aided manufacturing (CAD/CAM) technologies. The present technique not only improves the guide fabrication process and the accuracy of screw‐channel drilling, but also has wide indications for implant restorations.
Infraestructura y calidad educativa entre los pueblos indígenas originarios sonorenses
Authors: Vera-Noriega José Ángel , Luque-Agraz Diana , Bautista-Hernández Gildardo 
Published: March 2016

<p>El objetivo del artículo se centra en el análisis de la percepción entre los pueblos indígenas originarios de Sonora sobre la infraestructura y calidad educativa para la formación de sus descendientes. Participaron 1,.329 personas con un promedio de 50 años de edad y desviación estándar de 16 años de edad, 928 mujeres y 401 hombres. Los resultados indican la prevalencia de una percepción de falta de infraestructura para mejorar la calidad educativa de los niños y jóvenes indígenas. Además, se encontraron diferencias entre los siete pueblos indígenas con respecto a la problemática educativa. Los grupos que mejor están ubicados, en cuanto a acceso educativo, son aquellos que exhiben mayor desplazamiento de su lengua materna.</p>
Relación actitudes-conocimientos sobre diversidad sexual en una muestra universitaria colombiana
Authors: Franco-Morales Freddy , Correa-Molina Enrique , Venet Michèle , Pérez-Bedoya Santiago 
Published: March 2016

<p>Este artículo de investigación se interesa en las relaciones entre las actitudes del futuro personal docente hacia la homosexualidad y la bisexualidad, y sus conocimientos sobre roles sexuales, comportamientos sexuales y realidades de jóvenes homosexuales y bisexuales. Una muestra de 114 estudiantes universitarios en educación respondió un cuestionario para identificar sus conocimientos y actitudes. Los resultados de los análisis descriptivos evidencian que la muestra tiene poco conocimiento sobre los temas evaluados y una actitud “ni positiva ni negativa” hacia la homosexualidad y la bisexualidad. Además, los resultados de los análisis de correlación establecen una relación positiva entre las actitudes y los conocimientos aquí tratados. </p>
Complete Genome Sequence of
Bacillus methylotrophicus
Strain B25, a Potential Plant Growth-Promoting Rhizobacterium
Authors: Gerbore Jonathan , Brutel Aline , Lemainque Arnaud , Mairey Barbara , Médigue Claudine , Vallenet David , Lefort Francois , Grizard Damien 
Published: March 2016

ABSTRACT

The complete genome of
Bacillus methylotrophicus
strain B25, isolated in Switzerland, was sequenced. Its size is 3.85 Mb, and several genes that may contribute to plant growth-promoting activities were identified
in silico
.
Genome Sequences of
Staphylococcus hominis
Strains ShAs1, ShAs2, and ShAs3, Isolated from the Asian Malaria Mosquito
Anopheles stephensi
Authors: Hughes Grant L. , Raygoza Garay Juan Antonio , Koundal Vikas , Rasgon Jason L. , Mwangi Michael M. 
Published: March 2016

ABSTRACT

Staphylococcus hominis
is a culturable component of the bacterial microbiome of
Anopheles stephensi
. Here, we present the annotated draft genome sequences of three
S. hominis
isolates from
A. stephensi
. These genomic resources will facilitate experiments to further our understanding of the role of bacteria in mosquito biology.
Genome Sequence of
Stenotrophomonas maltophilia
Strain SmAs1, Isolated From the Asian Malaria Mosquito
Anopheles stephensi
Authors: Hughes Grant L. , Raygoza Garay Juan Antonio , Koundal Vikas , Rasgon Jason L. , Mwangi Michael M. 
Published: March 2016

ABSTRACT

An isolate of
Stenotrophomonas maltophilia
was cultured from the Asian malaria vector
Anopheles stephensi
. Here, we present the annotated draft genome sequence of this
S. maltophilia
strain. This genomic resource will facilitate further characterization of bacteria associated with mosquitoes.
Draft Genome Sequence of
Thermodesulfovibrio aggregans
TGE-P1
T
, an Obligately Anaerobic, Thermophilic, Sulfate-Reducing Bacterium in the Phylum
Nitrospirae
Authors: Matsuura Norihisa , Ohashi Akiko , Tourlousse Dieter M. , Sekiguchi Yuji 
Published: March 2016

ABSTRACT

We report a high-quality draft genome sequence of the type strain (TGE-P1
T
) of
Thermodesulfovibrio aggregans
, an obligately anaerobic, thermophilic, sulfate-reducing bacterium in the phylum
Nitrospirae
. The genome comprises 2.00 Mb in 16 contigs (3 scaffolds), has a G+C content of 34.5%, and contains 1,998 predicted protein-encoding genes.
Genome Sequences of
Listeria monocytogenes
Strains Responsible for Cheese- and Cooked Ham Product-Associated Swiss Listeriosis Outbreaks in 2005 and 2011
Authors: Tasara Taurai , Klumpp Jochen , Bille Jacques , Stephan Roger 
Published: March 2016

ABSTRACT

The complete genome sequences of three
Listeria monocytogenes
serotype 1/2a strains, Lm 3136, Lm 3163, and Lm N1546, which were responsible for listeriosis outbreaks in 2005 and 2011 in Switzerland, are presented here.
Complete Coding Genome Sequence of a Putative Novel Teschovirus Serotype 12 Strain
Authors: Cano-Gómez C. , Jiménez-Clavero M. A. 
Published: March 2016

ABSTRACT
Porcine teschoviruses are ubiquitous and prevalent viruses generally harmless to their hosts, the suids. Here, we report the first complete coding genome sequence of a putative new serotype of porcine teschovirus (PTV-12), strain CC25, isolated from fecal material from a healthy pig in Spain.
Draft Genome Sequence of
Pseudoalteromonas
sp. Strain XI10 Isolated from the Brine-Seawater Interface of Erba Deep in the Red Sea
Authors: Zhang Guishan , Fauzi Haroon Mohamed , Zhang Ruifu , Hikmawan Tyas , Stingl Ulrich 
Published: March 2016

ABSTRACT

Pseudoalteromonas
sp. strain XI10 was isolated from the brine-seawater interface of Erba Deep in the Red Sea, Saudi Arabia. Here, we present the draft genome sequence of strain XI10, a gammaproteobacterium that synthesizes polysaccharides for biofilm formation when grown in liquid culture.
Draft Genome Sequences of Two
Thiomicrospira
Strains Isolated from the Brine-Seawater Interface of Kebrit Deep in the Red Sea
Authors: Zhang Guishan , Fauzi Haroon Mohamed , Zhang Ruifu , Hikmawan Tyas , Stingl Ulrich 
Published: March 2016

ABSTRACT

Two
Thiomicrospira
strains, WB1 and XS5, were isolated from the Kebrit Deep brine-seawater interface in the Red Sea, Saudi Arabia. Here, we present the draft genome sequences of these gammaproteobacteria
,
which both produce sulfuric acid from thiosulfate in culture.
Whole-Genome Sequence of
Bradyrhizobium elkanii
Strain UASWS1015, a Highly Ammonia-Tolerant Nitrifying Bacterium
Authors: Crovadore Julien , Calmin Gautier , Cochard Bastien , Chablais Romain , Lefort François 
Published: March 2016

ABSTRACT

Bradyrhizobium elkanii
UASWS1015 was isolated from a sewage plant in Switzerland. Its genome indicates that it is fully equipped for ammonia assimilation and aromatic compound degradation, and it displays a large type IV secretion system, which characterizes plant-associated microbes. Totally deprived of toxins, it could be considered for agricultural and environmental uses.
Draft Genome Sequence of
Cobetia
sp. UCD-24C, Isolated from Roots and Leaves of the Seagrass
Zostera marina
Authors: Alexiev Alexandra , Krusor Megan L. , Jospin Guillaume , Lang Jenna M. , Eisen Jonathan A. , Coil David A. 
Published: March 2016

ABSTRACT

Here, we present the 4,230,758-bp draft genome for
Cobetia
sp. UCD-24C. This strain was isolated from
Zostera marina
roots collected in Woods Hole, Massachusetts, USA.
What factors are important for deprescribing in Australian long-term care facilities? Perspectives of residents and health professionals
Authors: Turner Justin P , Edwards Susan , Stanners Melinda , Shakib Sepehr , Bell J Simon 
Published: March 2016

ObjectivesPolypharmacy and multimorbidity are common in long-term care facilities (LTCFs). Reducing polypharmacy may reduce adverse events and maintain quality of life. Deprescribing refers to reducing medications after consideration of therapeutic goals, benefits and risks, and medical ethics. The objective was to use nominal group technique (NGT) to generate then rank factors that general medical practitioners (GPs), nurses, pharmacists and residents or their representatives perceive are most important when deciding whether or not to deprescribe medications.DesignQualitative research using NGT.SettingParticipants were invited if they worked with, or resided in LTCFs across metropolitan and regional South Australia.Participants11 residents/representatives, 19 GPs, 12 nurses and 14 pharmacists participated across six separate groups.MethodsIndividual groups of GPs, nurses, pharmacists and residents/representatives were convened. Using NGT each group ranked factors perceived to be most important when deciding whether or not to deprescribe. Then, using NGT, the prioritised factors from individual groups were discussed and prioritised by a multidisciplinary metropolitan and regional group comprised of resident representatives, GPs, nurses and pharmacists.ResultsNo two groups had the same priorities. GPs ranked ‘evidence for deprescribing’ and ‘communication with family/resident’ as most important factors. Nurses ranked ‘GP receptivity to deprescribing’ and ‘nurses ability to advocate for residents’ as most important. Pharmacists ranked ‘clinical appropriateness of therapy’ and ‘identifying residents’ goals of care’ as most important. Residents ranked ‘wellbeing of the resident’ and ‘continuity of nursing staff’ as most important. The multidisciplinary groups ranked ‘adequacy of medical and medication history’ and ‘identifying residents’ goals of care’ as most important.ConclusionsWhile each group prioritised different factors, common and contrasting factors emerged. Future deprescribing interventions need to consider the similarities and differences within the range of factors prioritised by residents and health professionals.
Health service utilisation for anogenital warts in Ontario, Canada prior to the human papillomavirus (HPV) vaccine programme introduction: a retrospective longitudinal population-based study
Authors: Guerra Fiona M , Rosella Laura C , Dunn Sheila , Wilson Sarah E , Chen Cynthia , Deeks Shelley L 
Published: March 2016

ObjectiveTrends in occurrence of anogenital warts (AGWs) can provide early evidence of human papillomavirus (HPV) vaccination programme impact on preventing HPV infection and HPV-induced lesions. The objective of this study was to provide a baseline of AGW epidemiology in Ontario prior to the introduction of the publicly-funded school-based HPV vaccination programme in September 2007.Setting and participantsAs a retrospective longitudinal population-based study, we used health administrative data as a proxy to estimate incident AGWs and total health service utilisation (HSU) for AGWs for all Ontario residents 15 years and older with valid health cards between 1 April 2003 and 31 March 2007.Outcome measuresThe outcome of interest was AGW healthcare utilisation identified using the International Classification of Diseases, 10th revision (ICD-10) diagnostic code for AGWs, as well as an algorithm for identifying AGW physician office visits in a database with a unique system of diagnostic and procedural codes. An AGW case was considered incident if preceded by 12 months without HSU for AGWs. Time trends by age group and sex were analysed.ResultsBetween fiscal years 2003 and 2006, we identified 123 247 health service visits for AGWs by 51 436 Ontario residents 15 years and older. Incident AGWs peaked in females and males in the 21–23 year age group, at 3.74 per 1000 and 2.81 per 1000, respectively. HSU for AGWs peaked in females and males within the 21–23 year age group, at 9.34 per 1000 and 7.22 per 1000, respectively.ConclusionsTo the best of our knowledge, this is the first population-based study of AGW incidence and HSU in Ontario. The sex and age distribution of individuals with incident and prevalent AGWs in Ontario was similar to that of other provinces before HPV vaccine programme implementation in Canada.
Past speculations of the future: a review of the methods used for forecasting emerging health technologies
Authors: Doos Lucy , Packer Claire , Ward Derek , Simpson Sue , Stevens Andrew 
Published: March 2016

ObjectivesForecasting can support rational decision-making around the introduction and use of emerging health technologies and prevent investment in technologies that have limited long-term potential. However, forecasting methods need to be credible. We performed a systematic search to identify the methods used in forecasting studies to predict future health technologies within a 3–20-year timeframe. Identification and retrospective assessment of such methods potentially offer a route to more reliable prediction.DesignSystematic search of the literature to identify studies reported on methods of forecasting in healthcare.ParticipantsPeople are not needed in this study.Data sourcesThe authors searched MEDLINE, EMBASE, PsychINFO and grey literature sources, and included articles published in English that reported their methods and a list of identified technologies.Main outcome measureStudies reporting methods used to predict future health technologies within a 3–20-year timeframe with an identified list of individual healthcare technologies. Commercially sponsored reviews, long-term futurology studies (with over 20-year timeframes) and speculative editorials were excluded.Results15 studies met our inclusion criteria. Our results showed that the majority of studies (13/15) consulted experts either alone or in combination with other methods such as literature searching. Only 2 studies used more complex forecasting tools such as scenario building.ConclusionsThe methodological fundamentals of formal 3–20-year prediction are consistent but vary in details. Further research needs to be conducted to ascertain if the predictions made were accurate and whether accuracy varies by the methods used or by the types of technologies identified.
Development and validation of a simple lupus severity index using ACR criteria for classification of SLE
Authors: Bello Ghalib A , Brown Michael A , Kelly Jennifer A , Thanou Aikaterini , James Judith A , Montgomery Courtney G 
Published: March 2016

ObjectiveTo develop a simple systemic lupus erythematosus (SLE) severity index that requires knowledge of only American College of Rheumatology (ACR) criteria and subcriteria.MethodsThis study used demographic, mortality and medical records data of 1915 patients with lupus from the Lupus Family Registry and Repository. The data were randomly split (2:1 ratio) into independent training and validation sets. A logistic regression with ridge penalty was used to model the probability of being prescribed major immunosuppressive drugs—a surrogate indicator of lupus severity. ACR criteria and subcriteria were used as predictor variables in this model, and the resulting regression coefficient estimates obtained from the training data were used as item weightings to construct the severity index.ResultsThe resulting index was tested on the independent validation dataset and was found to have high predictive accuracy for immunosuppressive use and early mortality. The index was also found to be strongly correlated with a previously existing severity score for lupus. In addition, demographic factors known to influence lupus severity (eg, age of onset, gender and ethnicity) all showed robust associations with our severity index that were consistent with observed clinical trends.ConclusionsThis new index can be easily computed using ACR criteria, which may be among the most readily available data elements from patient medical records. This tool may be useful in lupus research, especially large dataset analyses to stratify patients by disease severity, an important prognostic indicator in SLE.
Structure-dielectric property relationship in Nb-doped Ca4La2Ti5O17 ceramics
Authors: Muhammad Raz , Iqbal Yaseen 
Published: March 2016

A number of compounds in the [Formula: see text] (x = 0–2) series were prepared through a mixed oxide solid-state sintering route. These compounds crystallized into orthorhombic (Pbnm) structure. The relative permittivity [Formula: see text] and temperature coefficient of resonance frequency [Formula: see text] depended on the polarizability of constituent cations while the quality factor [Formula: see text] was affected by ordering of cations. These compounds exhibited promising [Formula: see text] (74–90) and [Formula: see text] (10[Formula: see text]208–14[Formula: see text]116 GHz); however, the observed high [Formula: see text] (157–542 ppm/[Formula: see text]C) requires further investigations to tune it through zero.
Mutant p53 Promotes Epithelial Ovarian Cancer by Regulating Tumor Differentiation, Metastasis, and Responsiveness to Steroid Hormones
Authors: Ren Yi A. , Mullany Lisa K. , Liu Zhilin , Herron Alan J. , Wong Kwong-Kwok , Richards JoAnne S. 
Published: March 2016

Abstract
Mutations in the tumor protein p53 (TP53) are the most frequently occurring genetic events in high-grade ovarian cancers, especially the prevalence of the Trp53R172H-mutant allele. In this study, we investigated the impact of the Trp53R172H-mutant allele on epithelial ovarian cancer (EOC) in vivo. We used the Pten/KrasG12D–mutant mouse strain that develops serous EOC with 100% penetrance to introduce the mutant Trp53R172H allele (homolog for human Trp53R172H). We demonstrate that the Trp53R172H mutation promoted EOC but had differential effects on disease features and progression depending on the presence or absence of the wild-type (WT) TP53 allele. Heterozygous WT/Trp53R172H alleles facilitated invasion into the ovarian stroma, accelerated intraperitoneal metastasis, and reduced TP53 transactivation activity but retained responsiveness to nutlin-3a, an activator of WT TP53. Moreover, high levels of estrogen receptor α in these tumors enhanced the growth of both primary and metastatic tumors in response to estradiol. Ovarian tumors homozygous for Trp53R172H mutation were undifferentiated and highly metastatic, exhibited minimal TP53 transactivation activity, and expressed genes with potential regulatory functions in EOC development. Notably, heterozygous WT/Trp53R172H mice also presented mucinous cystadenocarcinomas at 12 weeks of age, recapitulating human mucinous ovarian tumors, which also exhibit heterozygous TP53 mutations (∼50%–60%) and KRAS mutations. Therefore, we present the first mouse model of mucinous tumor formation from ovarian cells and supporting evidence that mutant TP53 is a key regulator of EOC progression, differentiation, and responsiveness to steroid hormones. Cancer Res; 76(8); 2206–18. ©2016 AACR.
STING Promotes the Growth of Tumors Characterized by Low Antigenicity via IDO Activation
Authors: Lemos Henrique , Mohamed Eslam , Huang Lei , Ou Rong , Pacholczyk Gabriela , Arbab Ali S. , Munn David , Mellor Andrew L. 
Published: March 2016

Abstract
Cytosolic DNA sensing is an important process during the innate immune response that activates the stimulator of interferon genes (STING) adaptor and induces IFN-I. STING incites spontaneous immunity during immunogenic tumor growth and accordingly, STING agonists induce regression of therapy-resistant tumors. However DNA, STING agonists, and apoptotic cells can also promote tolerogenic responses via STING by activating immunoregulatory mechanisms such as indoleamine 2,3 dioxygenase (IDO). Here, we show that IDO activity induced by STING activity in the tumor microenvironment (TME) promoted the growth of Lewis lung carcinoma (LLC). Although STING also induced IDO in tumor-draining lymph nodes (TDLN) during EL4 thymoma growth, this event was insufficient to promote tumorigenesis. In the LLC model, STING ablation enhanced CD8+ T-cell infiltration and tumor cell killing while decreasing myeloid-derived suppressor cell infiltration and IL10 production in the TME. Depletion of CD8+ T cells also eliminated the growth disadvantage of LLC tumors in STING-deficient mice, indicating that STING signaling attenuated CD8+ T-cell effector functions during tumorigenesis. In contrast with native LLC tumors, STING signaling neither promoted growth of neoantigen-expressing LLC, nor did it induce IDO in TDLN. Similarly, STING failed to promote growth of B16 melanoma or to induce IDO activity in TDLN in this setting. Thus, our results show how STING-dependent DNA sensing can enhance tolerogenic states in tumors characterized by low antigenicity and how IDO inhibition can overcome this state by attenuating tumor tolerance. Furthermore, our results reveal a greater complexity in the role of STING signaling in cancer, underscoring how innate immune pathways in the TME modify tumorigenesis in distinct tumor settings, with implications for designing effective immunotherapy trials. Cancer Res; 76(8); 2076–81. ©2016 AACR.
GSK-3 Inhibition Sensitizes Acute Myeloid Leukemia Cells to 1,25D-Mediated Differentiation
Authors: Gupta Kalpana , Stefan Tammy , Ignatz-Hoover James , Moreton Stephen , Parizher Gary , Saunthararajah Yogen , Wald David N. 
Published: March 2016

Abstract
1,25-dihydroxyvitamin D3 (1,25D), the biologically active form of vitamin D, is widely considered a promising therapy for acute myeloid leukemia (AML) based on its ability to drive differentiation of leukemic cells. However, clinical trials have been disappointing in part to dose-limiting hypercalcemia. Here we show how inhibiting glycogen synthase kinase 3 (GSK3) can improve the differentiation response of AML cells to 1,25D-mediated differentiation. GSK3 inhibition in AML cells enhanced the differentiating effects of low concentrations of 1,25D. In addition, GSK3 inhibition augmented the ability of 1,25D to induce irreversible growth inhibition and slow the progression of AML in mouse models. Mechanistic studies revealed that GSK3 inhibition led to the hyperphosphorylation of the vitamin D receptor (VDR), enabling an interaction between VDR and the coactivator, SRC-3 (NCOA3), thereby increasing transcriptional activity. We also found that activation of JNK-mediated pathways in response to GSK3 inhibition contributed to the potentiation of 1,25D-induced differentiation. Taken together, our findings offer a preclinical rationale to explore the repositioning of GSK3 inhibitors to enhance differentiation-based therapy for AML treatment. Cancer Res; 76(9); 2743–53. ©2016 AACR.