bluuebunny/crossref_metadata_2025_split huggingface dataset:
Store-Operated Calcium Entries Control Neural Stem Cell Self-Renewal in the Adult Brain Subventricular Zone
Authors: Domenichini Florence , Terrié Elodie , Arnault Patricia , Harnois Thomas , Magaud Christophe , Bois Patrick , Constantin Bruno , Coronas Valérie 
Published: January 2018

Abstract
The subventricular zone (SVZ) is the major stem cell niche in the brain of adult mammals. Within this region, neural stem cells (NSC) proliferate, self-renew and give birth to neurons and glial cells. Previous studies underlined enrichment in calcium signaling-related transcripts in adult NSC. Because of their ability to mobilize sustained calcium influxes in response to a wide range of extracellular factors, store-operated channels (SOC) appear to be, among calcium channels, relevant candidates to induce calcium signaling in NSC whose cellular activities are continuously adapted to physiological signals from the microenvironment. By Reverse Transcription Polymerase Chain Reaction (RT-PCR), Western blotting and immunocytochemistry experiments, we demonstrate that SVZ cells express molecular actors known to build up SOC, namely transient receptor potential canonical 1 (TRPC1) and Orai1, as well as their activator stromal interaction molecule 1 (STIM1). Calcium imaging reveals that SVZ cells display store-operated calcium entries. Pharmacological blockade of SOC with SKF-96365 or YM-58483 (also called BTP2) decreases proliferation, impairs self-renewal by shifting the type of SVZ stem cell division from symmetric proliferative to asymmetric, thereby reducing the stem cell population. Brain section immunostainings show that TRPC1, Orai1, and STIM1 are expressed in vivo, in SOX2-positive SVZ NSC. Injection of SKF-96365 in brain lateral ventricle diminishes SVZ cell proliferation and reduces the ability of SVZ cells to form neurospheres in vitro. The present study combining in vitro and in vivo approaches uncovers a major role for SOC in the control of SVZ NSC population and opens new fields of investigation for stem cell biology in health and disease.
Prevalence of most common human pathogenicCampylobacterspp. in dogs and cats in Styria, Austria
Authors: Pölzler Thomas , Stüger Hans‐Peter , Lassnig Heimo 
Published: January 2018

AbstractThe aim of this study was to determine the frequency of occurrence of most common human pathogenicCampylobacterspecies,Campylobacter jejuni (C. jejuni)andCampylobacter coli (C. coli), in dogs and cats in Styria, Austria. In the period from April 2010 to April 2012, 842 faecal samples from dogs and cats from Styria, Austria were examined forCampylobacter (C.)species (spp.). All samples were subjected to qualitative microbiological culture testing, and additionally, some of them have been studied using qualitative real‐timePCR. In microbiological culture, 5.9% of all samples investigated wereC. spp. positive. With 3.1% out of positive samples,C. jejuniwas the most common type.Campylobacter upsaliensis (C. upsaliensis)was detected only in 0.5% of the samples. The remaining positive samples (2.4%) were classified asC. species (sp.).C. colicould not be found in any of the samples. A higher prevalence ofC. jejuniwas found in kittens with 14.3% and in diarrhoeic dogs (7.4%) and cats (23.8%). The real‐timePCRrevealed for dogs and cats together, 27% ofC. jejuni‐positive faecal and 8% positive faecal swap samples. The obtainedC. jejunistrains underwent antibiotic resistance testing using three different tests (agar diffusion,MICtesting and E‐test) with different numbers of antibiotics. From the antibiotics used in this study, several showed high test‐dependent resistance rates (cephalexin, cefovecin, kanamycin, sulfamethoxazole/trimethoprim, penicillin G, ciprofloxacin, enrofloxacin, marbofloxacin, nalidixic acid). Overall, the prevalence ofC. spp. in this study was very low compared to others, with the exception ofC. jejuniin kittens and diarrhoeic animals. The results of the real‐timePCRsuggest that the rate of colonization ofC. jejuniwas actually higher than the results of the culture showed. As the resistance rates ofC. jejuniisolates partly were very high, possible transmission of (multi‐) resistantC. jejunistrains to humans especially from kittens and diarrhoeic animals must be expected.
Responses of the photosynthetic apparatus of Abies koreana to drought under different light conditions
Authors: Je Sun Mi , Kim Sun Hee , Woo Soo Young 
Published: January 2018

AbstractThe threat of drought to trees is predicted to increase due to global warming. In a forest stand, the physiological responses of trees can differ depending on the light conditions. We analyzed photosynthetic rate, photopigment, and chlorophyll a fluorescence transient (OJIP test) of Abies koreana E.H. Wilson, under different light (full sunlight and 35 and 75% shading) and water conditions (well‐watering, W; and no‐watering, NW) to examine the combined effect of light and water. After 21 days of no‐watering, we observed decreases in the photosynthetic rate and photopigment contents and quality, impairment of electron transfer from primary to secondary quinone acceptor, inactivation of reaction center, and lower photosynthetic performance index, especially under full sunlight. The time required for recovery after re‐watering was also slower under full sunlight. In conclusion, the adverse effects of drought on light absorption and utility of A. koreana in the photosynthetic process were much greater under high light intensity compared to shading conditions, which alleviated these effects.
Spiritual care training in healthcare: Does it really have an impact?
Authors: Daudt Helena , d'Archangelo Margo , Duquette Dominique 
Published: January 2018

AbstractObjectiveSpiritual care has formed an integral part of palliative care since its inception. People with advanced illnesses, however, frequently report that their spiritual needs are not attended to by their medical care team. The present study examines and describes the impact of a spiritual care training program on practice and cultural change in our Canadian hospice.MethodA qualitative case study approach was adopted to gather feedback from hospice staff and volunteers using purposive sampling. In-depth interviews were conducted, transcribed, and analyzed using thematic (semantic and latent) analysis.ResultOur data suggest that the program had a profound personal impact on attendees and contributed to a slight shift in practice patterns in our organization. Using a program not specifically tailored to our local and organizational cultural contexts resulted in some unanticipated challenges such as the range of tensions between personal and cultural boundaries. Although some people criticized parts of the program or questioned the program's value, a general agreement suggests that the program had a positive impact and meaningfully benefited our hospice. “What will happen next?” was the question most frequently voiced by interviewees.Significance of resultsAlthough the program may not have been a perfect fit for our organization, its use instigated a process of cultural change that unfolds today. The present study suggests that a systematic approach to spiritual care training that includes the concepts of workplace spirituality and sensitive practice offer useful frameworks for the development and implementation of spiritual care training in other institutions.
The dual-inhibitory effect of miR-338-5p on the multidrug resistance and cell growth of hepatocellular carcinoma
Authors: Zhao Yang , Chen Jing , Wei Wenxin , Qi Xinming , Li Chunzhu , Ren Jin 
Published: January 2018

AbstractChemotherapeutic treatments against hepatocellular carcinoma (HCC) are necessary for both inoperable patients to improve prospects for survival and surgery patients to improve the outcome after surgical resection. However, multidrug resistance (MDR) is a major obstacle to obtaining desirable results. Currently, increasing the chemotherapy sensitivity of tumor cells or discovering novel tumor inhibitors is an effective therapeutic strategy to solve this issue. In the present study, we uncovered the dual-inhibitory effect of miR-338-5p: on the one hand, it could downregulate ABCB1 expression and sensitize HCC cells to doxorubicin and vinblastine by directly targeting the 3′-untranslated region (3′-UTR) of ABCB1, while, on the other hand, it could suppress the proliferation of HCC cells by directly targeting the 3′-UTR of EGFR and reducing EGFR expression. Since EGFR regulates ABCB1 levels, the indirect action of miR-338-5p in ABCB1 modulation was revealed, in which miR-338-5p inhibits ABCB1 expression by targeting the EGFR/ERK1/2 signaling pathway. These data indicate that the miR-338-5p/EGFR/ABCB1 regulatory loop plays a critical role in HCC, and a negative correlation between miR-338-5p and EGFR or ABCB1 was also detected in HCC clinical samples. In conclusion, these findings reveal a critical role for miR-338-5p in the regulation of MDR and proliferation of HCC, suggesting the potential therapeutic implications of miR-338-5p in HCC treatment.
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Authors: Yoon Yeonsoo , Wang Dan , Tai Phillip W. L. , Riley Joy , Gao Guangping , Rivera-Pérez Jaime A. 
Published: January 2018

AbstractRecent advances using CRISPR-Cas9 approaches have dramatically enhanced the ease for genetic manipulation in rodents. Notwithstanding, the methods to deliver nucleic acids into pre-implantation embryos have hardly changed since the original description of mouse transgenesis more than 30 years ago. Here we report a novel strategy to generate genetically modified mice by transduction of CRISPR-Cas9 components into pre-implantation mouse embryos via recombinant adeno-associated viruses (rAAVs). Using this approach, we efficiently generated a variety of targeted mutations in explanted embryos, including indel events produced by non-homologous end joining and tailored mutations using homology-directed repair. We also achieved gene modification in vivo by direct delivery of rAAV particles into the oviduct of pregnant females. Our approach greatly simplifies the generation of genetically modified mice and, more importantly, opens the door for streamlined gene editing in other mammalian species.
Whole-genome transcriptomic insights into protective molecular mechanisms in metabolically healthy obese African Americans
Authors: Gaye Amadou , Doumatey Ayo P. , Davis Sharon K. , Rotimi Charles N. , Gibbons Gary H. 
Published: January 2018

AbstractSeveral clinical guidelines have been proposed to distinguish metabolically healthy obesity (MHO) from other subgroups of obesity but the molecular mechanisms by which MHO individuals remain metabolically healthy despite having a high fat mass are yet to be elucidated. We conducted the first whole blood transcriptomic study designed to identify specific sets of genes that might shed novel insights into the molecular mechanisms that protect or delay the occurrence of obesity-related co-morbidities in MHO. The study included 29 African-American obese individuals, 8 MHO and 21 metabolically abnormal obese (MAO). Unbiased transcriptome-wide network analysis was carried out to identify molecular modules of co-expressed genes that are collectively associated with MHO. Network analysis identified a group of 23 co-expressed genes, including ribosomal protein genes (RPs), which were significantly downregulated in MHO subjects. The three pathways enriched in the group of co-expressed genes are EIF2 signaling, regulation of eIF4 and p70S6K signaling, and mTOR signaling. The expression of ten of the RPs collectively predicted MHO status with an area under the curve of 0.81. Triglycerides/HDL (TG/HDL) ratio, an index of insulin resistance, was the best predictor of the expression of genes in the MHO group. The higher TG/HDL values observed in the MAO subjects may underlie the activation of endoplasmic reticulum (ER) and related-stress pathways that lead to a chronic inflammatory state. In summary, these findings suggest that controlling ER stress and/or ribosomal stress by downregulating RPs or controlling TG/HDL ratio may represent effective strategies to prevent or delay the occurrence of metabolic disorders in obese individuals.
Cardiac shock wave therapy promotes arteriogenesis of coronary micrangium, and ILK is involved in the biomechanical effects by proteomic analysis
Authors: Yang Wenhui , He Yan , Gan Lulu , Zhang Fan , Hua Baotong , Yang Ping , Liu Juan , Yang Li , Guo Tao 
Published: January 2018

AbstractCardiac Shock Wave Therapy (CSWT) improves myocardial perfusion and ameliorates cardiac remodeling after acute myocardial infarction (AMI), but the precise mechanisms remain obscure. Herein, we have applied CSWT to a rat model of AMI to demonstrate the arteriogenesis of coronary micrangium and protein expression changes in ischemic myocardium after CSWT. Four weeks after CSWT, the fraction shortening of rats was improved greatly and the cardiomyocyte apoptosis index was significantly lower than the AMI group (P < 0.05). Besides, the fibrotic area was markedly decreased in the CSWT group. In the infarction border zone, the thickness of smooth muscle layer was expanded apparently after CSWT. Label-free quantitative proteomic analysis and bioinformatics analysis revealed that the differentially expressed proteins were largely enriched in the focal adhesion signaling pathway. And integrin linked kinase (ILK) may be a key factor contributed to arteriogenesis of coronary micrangium during CSWT. In conclusion, non-invasive cardiac shock wave could promote arteriogenesis of coronary micrangium and alleviate myocardial apoptosis and fibrosis after AMI. Furthermore, focal adhesion signaling pathway may have a central role in the related signal network and ILK was closely related to the arteriogenesis of coronary micrangium during CSWT.
Early immune anergy towards recall antigens and mitogens in patients at onset of septic shock
Authors: Feuerecker M. , Sudhoff L. , Crucian B. , Pagel J.-I. , Sams C. , Strewe C. , Guo A. , Schelling G. , Briegel J. , Kaufmann I. , Choukèr A. 
Published: January 2018

AbstractThe pathology of sepsis is typically characterized by an infection and excessive initial inflammation including a cytokine storm, followed by a state of immune suppression or paralysis. This classical view of a two peak kinetic immune response is currently controversially discussed. This study was a sub-study of the randomized clinical Trial SISPCT registered withwww.clinicaltrials.gov(NCT00832039, Registration date: 29/01/2009). Blood samples from 76 patients with severe sepsis and septic shock were incubated for 48 h at 37 °Cin vitrowith bacterial or fungal recall-antigens or specific mitogen antigens within 24 hours of sepsis onset. Recall-antigen stimulation led to a severe dampening of normal cytokine release. This immunologic anergy was similarly observed after mitogen stimulation. Moreover, patients under hydrocortisone therapy or with lowered arterial oxygen tension had further reductions in cytokine levels upon B- and T-cell mitogen stimulation. This investigation reveals an early onset of immunoparalysis during sepsis. This immune incompetence in mounting an adequate response to further infections includes previously sensitized pathogens, as seen with recall-antigens. Also, the immune-suppressive role of hydrocortisone and low PaO2is highlighted. Aside from early broad-spectrum antimicrobial therapy, our findings reinforce the need for maximal immunological support and protection against further infections at the onset of sepsis.
Generalized statistical mechanics of cosmic rays: Application to positron-electron spectral indices
Authors: Yalcin G. Cigdem , Beck Christian 
Published: January 2018

AbstractCosmic ray energy spectra exhibit power law distributions over many orders of magnitude that are very well described by the predictions of q-generalized statistical mechanics, based on a q-generalized Hagedorn theory for transverse momentum spectra and hard QCD scattering processes. QCD at largest center of mass energies predicts the entropic index to be $$q=\frac{{\bf{13}}}{{\bf{11}}}$$
q
=

13

11

. Here we show that the escort duality of the nonextensive thermodynamic formalism predicts an energy split of effective temperature given by Δ $${\boldsymbol{k}}{\boldsymbol{T}}{\boldsymbol{=}}{\boldsymbol{\pm }}\frac{{\bf{1}}}{{\bf{10}}}{\boldsymbol{k}}{{\boldsymbol{T}}}_{{\boldsymbol{H}}}{\boldsymbol{\approx }}{\boldsymbol{\pm }}{\bf{18}}$$
k
T
=
±

1

10

k

T

H

≈
±

18
MeV, where T
H
is the Hagedorn temperature. We carefully analyse the measured data of the AMS-02 collaboration and provide evidence that the predicted temperature split is indeed observed, leading to a different energy dependence of the e+ and e− spectral indices. We also observe a distinguished energy scale E* ≈ 50 GeV where the e+ and e− spectral indices differ the most. Linear combinations of the escort and non-escort q-generalized canonical distributions yield excellent agreement with the measured AMS-02 data in the entire energy range.
Design and implementation of multi-signal and time-varying neural reconstructions
Authors: Nanda Sumit , Chen Hanbo , Das Ravi , Bhattacharjee Shatabdi , Cuntz Hermann , Torben-Nielsen Benjamin , Peng Hanchuan , Cox Daniel N. , De Schutter Erik , Ascoli Giorgio A. 
Published: January 2018

AbstractSeveral efficient procedures exist to digitally trace neuronal structure from light microscopy, and mature community resources have emerged to store, share, and analyze these datasets. In contrast, the quantification of intracellular distributions and morphological dynamics is not yet standardized. Current widespread descriptions of neuron morphology are static and inadequate for subcellular characterizations. We introduce a new file format to represent multichannel information as well as an open-source Vaa3D plugin to acquire this type of data. Next we define a novel data structure to capture morphological dynamics, and demonstrate its application to different time-lapse experiments. Importantly, we designed both innovations as judicious extensions of the classicSWCformat, thus ensuring full back-compatibility with popular visualization and modeling tools. We then deploy the combined multichannel/time-varying reconstruction system on developing neurons in live Drosophila larvae by digitally tracing fluorescently labeled cytoskeletal components along with overall dendritic morphology as they changed over time. This same design is also suitable for quantifying dendritic calcium dynamics and tracking arbor-wide movement of any subcellular substrate of interest.
Changing the Subject: The Place of Revisions in Grammatical Development
Authors: Rispoli Matthew 
Published: January 2018

PurposeThis article focuses on toddlers' revisions of the sentence subject and tests the hypothesis that subject diversity (i.e., the number of different subjects produced) increases the probability of subject revision.MethodOne-hour language samples were collected from 61 children (32 girls) at 27 months. Spontaneously produced, active declarative sentences (ADSs) were analyzed for subject diversity and the presence of subject revision and repetition. The number of different words produced, mean length of utterance, tense/agreement productivity score, and the number of ADSs were also measured.ResultsRegression analyses were performed with revision and repetition as the dependent variables. Subject diversity significantly predicted the probability of revision, whereas the number of ADSs predicted the probability of repetition.ConclusionThe results support the hypothesis that subject diversity increases the probability of subject revision. It is proposed that lexical diversity within specific syntactic positions is the primary mechanism whereby revision rates increase with grammatical development. The results underscore the need to differentiate repetition from revision in the classification of disfluencies.
Synthesis of Heterobenzylic Fluorides
Authors: Britton Robert , Meanwell Michael 
Published: January 2018

Fluorination at heterobenzylic positions can have a significant impact on basicity, lipophilicity, and metabolism of drug leads. As a consequence, the development of new methods to access heterobenzylic fluorides has particular relevance to medicinal chemistry. This short review provides a survey of common methods used to synthesize heterobenzylic fluorides and includes fluoride displacement reactions of previously functionalized molecules (e.g., deoxyfluorination and halide exchange) and electrophilic fluorination of resonance-stabilized heterobenzylic anions. In addition, recent advances in the direct fluorination of heterobenzylic C(sp3)–H bonds and monofluoromethylation of heterocyclic C(sp2)–H bonds are presented.1	Introduction2	Heterobenzylic Fluorides2.1	Deoxyfluorination2.2	Halide Exchange2.3	Electrophilic Fluorination of Heterobenzylic Anions2.4	Late Stage C–H Bond Fluorination2.5	Monofluoromethylation of C(sp2)–H Bonds3	Conclusions
Fascia Suture Technique and Suture-mediated Closure Devices: Systematic Review
Authors: Kostakis Ioannis , Moris Demetrios , Palla Viktoria-Varvara , Moulakakis Konstantinos , Karaolanis Georgios 
Published: January 2018

Background The aim of the present study is to review the available data on suture-mediated closure devices (SMCDs) and fascia suture technique (FST), which are alternatives for minimizing the invasiveness of percutaneous endovascular aortic aneurysm repair (p-EVAR) and reduce the complications related to groin dissections.
Methods The Medline, ClinicalTrials.gov, and Cochrane library – Cochrane Central Register of Controlled Trials (CENTRAL) databases were searched for publications regarding SMCD and FST between January 1999 and December 2016.
Results We review 37 original articles, 30 referring to SMCDs (Prostar XL and Proglide), which included 3,992 patients, and 6 articles referring to FST, which include 426 patients. The two techniques are compared only in one article (100 patients). The two types of SMCDs were Prostar and Proglide. In most studies on SMCDs, the reported technical success rates were between 89 and 100%, but the complication rates varied greatly between 0 and 25%. Concerning FST, the technical success rates were also high, ranging between 87 and 99%. However, intraoperative complication rates ranged between 1.2 and 13%, whereas postoperative complication rates varied from 0.9 to 6.2% for the short-term and from 1.9 to 13.6% for the long-term.
Conclusions SMCDs and FST seem to be effective and simple methods for closing common femoral artery (CFA) punctures after p-EVAR. FST can reduce the access closure time and the procedural costs with a quite short learning curve, whereas it can work as a bailout procedure for failed SMCDs suture. The few failures of the SMCDs and FST that may occur due to bleeding or occlusion can easily be managed.
Anti-N-Methyl-D-Aspartate Receptor Encephalitis in a 3-Year-Old Toddler with the Involvement of Severe Autonomic Dysfunctions in Gastrointestinal System: A Case Report
Authors: Liu Li-Ying , Wang Yang-Yang , Pang Ling-Yu , Zhang Gui-Xia , Zou Li-Ping 
Published: January 2018

AbstractAnti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is under-recognized in children and adults. It is characterized by prominent neurologic and psychiatric features at first onset. Autonomic manifestations are rarer in children than in adults. We report a 3-year-old male patient with rare manifestation of severe autonomic dysfunctions of the gastrointestinal system and atypical prodromal symptoms. The serum and CSF of the patient were positive for anti-NMDA receptor antibodies, and his electroencephalogram results showed an “extreme delta brush” pattern. The patient had completely recovered by the sixth-month assessment after receiving first-line immunomodulatory treatment. Thus, anti-NMDAR encephalitis should be considered in the differential diagnosis of children with mental status changes and widespread autonomic dysfunction, particularly if symptoms are accompanied by gastrointestinal dysmotility and anhidrosis.