bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leptin augments the inflammatory effect of Interleukin 1 Beta on synoviocytes mainly through NF-kB and STAT3 prompting its possible implementation in OA pathogenesis
Authors: Wayupat Atitaya , Kongtawelert Prachya , Pothacharoen Peraphan , Shwe Thuzar Hla , Phitak Thanyaluck 
Published: October 2024

Abstract

Background
Increasing evidences have shown high osteoarthritis (OA) incidence in both weight and non-weight bearing joints among obese patients. Since leptin level in synovial fluid of obese OA patients was elevated compared to that of healthy people, leptin may be a crucial contributing factor in pathogenesis of OA, especially for non-weight bearing joints, in obese patients. This study aimed to examine the effect of leptin on synovial inflammation in the presence or absence of IL-1β, known inducer of joint inflammation, and the plausible intracellular mechanisms in SW982 synoviocytes.
Methods and results
Leptin, in the physiological concentration, as low as 1 ng/mL, can induce the expression of inflammatory cytokines, IL-6 and IL-8, in synoviocytes by activating p65, p38, JNK, STAT1, and STAT3. In addition, the lowest pathological concentration of leptin can enhance the inflammatory effect of IL-1β resulting in significantly higher production of IL-6 and IL-8 via activation of p65 and STAT3, highlighting its role in pathogenesis of inflammation-related OA.
Conclusions
Our research discovered crosstalking of p65 and STAT3, it may have a significant role in the development of OA in the joints of obese people by enhancing inflammation through the activation of p65 and STAT3.
Evaluation of colorectal liver metastases using virtual monoenergetic images obtained from dual-layer spectral computed tomography
Authors: Bae Jae Seok , Yoon Jeong Hee , Kim Jae Hyun , Han Seungchul , Park Sungeun , Kim Se Woo 
Published: October 2024

Abstract

Purpose
To assess the potential of virtual monoenergetic images in assessing colorectal liver metastasis (CRLM) compared with conventional CT images
Methods
This single-center, retrospective study included 173 consecutive patients (mean age, 65.5 ± 10.6 years; 106 men) who underwent dual-layer spectral CT (DLSCT) between November 2016 and April 2021. Portal venous phase images were reconstructed using hybrid iterative reconstruction (iDose) and virtual monoenergetic imaging at 50 keV. Four radiologists independently and randomly reviewed the de-identified iDose and 50 keV images. Lesion detection, CRLM conspicuity, and CRLM diagnosis were compared between these images using a generalized estimating equation analysis. The reference standards used were histopathology and follow-up imaging findings.
Results
The study included 797 focal liver lesions, including 463 CRLMs (median size, 18.1 mm [interquartile range, 10.9–37.7 mm]). Lesion detection was better with 50 keV images than with iDose images (45.0% [95% CI: 39–50] vs 40.0% [95% CI: 34–46], P = .003). CRLM conspicuity was higher in the 50 keV images than in the iDose images (3.27 [95% CI: 3.09–3.46] vs 3.09 [95% CI: 2.90–3.28], P < .001). However, the specificity for diagnosing CRLM was lower with 50 keV images than with iDose images (94.5% [95% CI: 91.6–96.4] vs 96.0% [95% CI: 93.2–98.1], P = .022), whereas sensitivity did not differ significantly (77.6% [95% CI: 70.3–83.5] vs 76.9% [95% CI: 70.0–82.7], P = .736). Indeterminate lesions were more frequently noted in 50 keV images than in iDose images (13% [445/3188] vs 9% [313/3188], P = .005), and 56% (247/445) of the indeterminate lesions at 50 keV were not CRLMs.
Conclusion
The 50 keV images obtained from DLSCT were better than the iDose images in terms of CRLM conspicuity and lesion detection. However, 50 keV images did not improve CRLM diagnosis but slightly increased the reporting of indeterminate FLLs associated with CRLMs.
The Neural Correlates of Social Appraisal Dysfunction in Major Depressive Disorder: An Event-Related Potential Study
Authors: Li Miao , Li Jia-xv , Han Jia-min , Liu Xiao-hong , Gao Xue-zheng , Chen Li-min , Zhou Zhen-he , Zhou Hong-liang 
Published: October 2024

Abstract

Background: Research has demonstrated that some individuals with major depressive disorder (MDD) exhibit more distorted social appraisal than non-depressed individuals. This study aimed to explore the neuroelectrophysiological mechanisms of emotional processing bias in patients with MDD and thus to understand their functional properties of social appraisal.
Methods: Thirty-four patients with MDD and 34 healthy controls (HCs) were selected. The behavioral and event-related potentials (ERPs) data of the Socio-emotional Preference Task (SePT) were recorded and analyzed.
Results: The behavioral results showed that the MDD group showed longer reaction times (RTs) to both negative and positive stimuli compared to the HCs group, preferring negative stimuli. The ERP data indicated that the MDD group exhibited higher P200 amplitudes for negative and neutral stimuli compared to the HCs group. Additionally, they showed higher LPP amplitudes for negative and positive stimuli, with longer LPP latencies for negative stimuli. In the MDD group, multiple linear stepwise regression analysis showed that LPP amplitudes were positively correlated with RTs for positive stimuli and negatively correlated with RTs for negative stimuli. Conversely, P200 amplitudes were negatively correlated with RTs for negative stimuli but positively correlated with Hamilton Depression Rating Scale (HDRS-17) scores. Moreover, HDRS-17 scores positively correlated with the self-reported preference for negative stimuli but negatively correlated with the self-reported preference for positive stimuli.
Conclusion: Patients with MDD tend to choose negative information that is consistent with negative self-schema. The brain devotes more cognitive resources and longer RTs to processing negative stimuli, starting from the early stages (P200) and extending into the later stages (LPP), there is a tendency for processing fixation. RTs of patients with MDD to negative and positive stimuli can predict the amplitudes of LPP. Additionally, their preference for negative stimuli and avoidance of positive stimuli can predict depressive symptom severity.
Trial of Labor and Neonatal Outcomes in Extreme prematurity &lt;28 Weeks' Gestation
Authors: Gilboa Itamar , Gabbai Daniel , Yogev Yariv , Lavie Anat , Attali Emmanuel , Zaltz Noa , Herzlich Jacky , Melamed Ben , Hiersch Liran , Lavie Michael 
Published: October 2024

Abstract

BACKGROUND
The optimal mode of delivery for extremely preterm infants remains a subject of debate, particularly concerning the potential benefits of planned cesarean delivery.
OBJECTIVE
This study aimed to compare adverse neonatal outcomes between planned cesarean delivery and a trial of labor for singleton infants born before 28 weeks of gestation.
STUDY DESIGN
We conducted a retrospective cohort study of all singleton pregnancies delivered between 240/7 and 276/7 weeks of gestation at a single university-affiliated tertiary medical center. Patients were categorized into two groups: patients who opted a trial of vaginal delivery (TOL group) and those who had planned cesarean delivery (CD group). The primary outcome included neonatal death before discharge and severe neurological injury. Postnatal outcomes were recorded during hospitalization before discharge, and data were analyzed using an intention-to-treat analysis. Additional sub-analyses included women with spontaneous onset of labor without maternal or neonatal delivery indications and those within the gestational age range of 250/7 to 276/7 weeks.
RESULTS
A total of 199 patients were eligible for inclusion: 64 opted a trial of vaginal delivery (TOL group), while 135 underwent planned cesarean delivery (CD group). Within the TOL group, there was a higher incidence of delivery at 240/7- 246/7 weeks of gestation. Five women (7.8%) in the TOL group underwent intrapartum urgent CD. No disparities in the rates of primary or overall composite outcomes were observed between the TOL and CD groups (26.6% vs. 31.9%, p = 0.448 and 85.9% vs. 90.4%, p = 0.352, respectively). Adjusted multivariate analysis did not find significance between a trial of labor or planned cesarean delivery.
CONCLUSIONS
In extreme preterm births between 24–28 weeks of gestation no difference was found for the risk for adverse neonatal outcomes between those who had a trial of labor and those who underwent planned cesarean delivery.
Dissecting the association between breast cancer and scar conditions and fibrosis of skin : a bidirectional Mendelian randomization study
Authors: Chen Jinhao , Wang Qianru , Zhan Lijun , Lv Yafeng , Li Xun , Cao Chunyu 
Published: October 2024

Abstract

Background
Scar hyperplasia and skin fibrosis following breast cancer operation has long been recognized as one of the major effects affecting patients' quality of life. However, there is currently a lack of direct evidence examining the impact of breast cancer on scar conditions and fibrosis of skin.
Methods
In this study, a two-way, two-sample Mendelian randomisation (MR) approach utilising pooled data from a genome-wide association study (GWAS) we employed to investigate the potential causal relationship between breast cancer (BC) and scar conditions and fibrosis of skin.In forward MR, it was postulated that BC was the exposure factor, whereas in reverse MR, scar conditions and fibrosis of skin were posited as the exposure factors. To enhance the reliability of the results, two databases (finna-a-L12_SCARCONDITIONS; ukb-b-11403 ) were employed to examine these factors.In order to explore the potential relationship between BC and scar conditions and fibrosis of skin, various analytical techniques were utilised, including random effects inverse variance weighted (IVW) and MR-Egger analyses.Furthermore, sensitivity analyses and assessments of heterogeneity and multiplicity were conducted to enhance the reliability of the results.
Results
The MR analysis indicates that BC is associated with an elevated risk of scar conditions and fibrosis of skin(OR = 1.310, 95%CI = 1.052–1.630, p = 0.015 ) and(OR = 1.00047, 95%CI = 1.000016–1.000928, p = 0.042 ) .In contrast, the inverse MR analysis did not identify scar conditions and fibrosis of skin as a potential factor influencing BC risk.Furthermore, sensitivity analyses of these results demonstrated the absence of evidence for heterogeneity or pleiotropy.
Conclusion
This study showed that a positive causal relationship exists between BC and scar conditions and fibrosis of skin in European populations. This study also offers a novel perspective on the mechanism study of postoperative scar formation in breast cancer patients, and provides a novel basis for clinical assessment of patients' prognosis based on the status of surgical scarring in BC and the degree of dermal fibrosis.
Prevalence of mandibular third molar impaction, associated pathologies, and correlation with Temporomandibular joint morphology in Spanish Population: A panoramic radiography study
Authors: ASSIRI HASSAN , Estrugo-Devesa Albert , Egido-Moreno Sonia , Rosello-LLabres Xavier , Hameed Mohammad Shahul , Alqarni Abdullah , Lopez Jose Lopez 
Published: October 2024

Abstract

Background
mandibular third molar is considered as the most frequent impacted tooth in the oral cavity. Its cooccurrence could lead to some impacts including the temromandbular joint symptoms. Therefore, the present study aimed to assess the prevalence of impacted mandibular third molar (IMTM), any associated pathologies, and their correlation with temporomandibular joint (TMJ) morphology in the Spanish population.
Methods
the existing records of orthopantomography images; panoramic images (OPGs) of patients visiting the Dental Hospital of the University of Barcelona, aged 18–40 years, having IMTM, were assessed, and interpreted by experienced Oral Radiologists and retrieved from September 2021 and May 2023. OPGs were screened for the type of impaction according to Winter classification system, any associated pathologies, and shape of mandibular condyle.
Results
out of 80 OPGs, 60% were females, and the majority (53.8%) were between 18 and 28 years of age. The prevalence rate of IMTM was 86.88%, with the left side commonly involved. On both sides, oval-shaped condyle and vertical IMTM were the most common, with dental caries and bone loss being the commonly observed pathologies. 15% cases showed sclerotic changes on both sides of TMJ. A statistically non-significant difference (P > 0.05) was observed between associated pathologies and condyle shape. Vertical and mesioangular, followed by horizontally impacted mandibular third molars were observed to be the most prevalent types of impaction, resulting in a statistically non-significant association (P > 0.05) with condylar shape.
Conclusion
The pattern of IMTM in the Spanish population showed the prevalence of vertical impaction, mainly on the left side of the mandible, with a female predominance. The commonly associated pathological changes are dental caries and bone loss, revealing a significant relation with the type of impaction and condyle shape.
Effect of day 3 embryo cell number on the pregnancy and neonatal outcomes of day 4 single embryo transfer from fresh cycles
Authors: Tao Linlin , Zheng Bo , Li Guozhen , Geng Yasong , Guo Yuying , Dai Haoyang , Wang Shusong , Dai Fangfang 
Published: October 2024

Abstract

Background
The aim of this retrospective cohort study was to assess the impact of day 3(D3) embryo cell number on the clinical pregnancy and neonatal outcomes of day 4(D4) single embryo transfer in fresh cycles.
Methods
The study included 431 day 4 single embryo transfer in fresh cycles conducted between December 2018 and June 2023. These cycles were divided into three groups according to the day 3 embryo cell number: 248 cycles in the 7 ~ 9-cell group, 149 cycles in the 10 ~ 13-cell group and 34 cycles in the >13-cell group, and clinical pregnancy outcomes and neonatal outcomes were compared among the three groups.
Results
The clinical outcomes with 10 ~ 13-cell were significantly higher than those of the 7 ~ 9-cell group, regardless of whether the female age was < 30 or ≥ 30 years. The same result could be found when the insemination pattern was IVF, and when the transferred embryos were the grade of complete fusion. There were no differences in neonatal outcomes between different groups.After adjusting for confounding factors, the 7 ~ 9-cell group was associated with lower clinical pregnancy rates and live birth rates compared with the 10 ~ 13-cell group (clinical pregnancy rates: aOR 0.521, 95% CI -1.198~-0.105, P = 0.019; LBR: aOR 0.539, 95% CI -1.122~-0.114, P = 0.016)
Conclusion
The cell number of D3 embryos can be an important reference indicator for D4 embryo selection. When performing day 4 single embryo transfer in fresh cycles, embryos with 10 ~ 13-cell on D3 can be preferentially selected for transplantation to enhance clinical outcomes, especially when the insemination pattern is IVF,and when the transferred embryos are the grade of compaction stage
Predictions of psychiatric hospitalization and emergency department utilization in commercially insured late middle-aged adults with depression
Authors: Xi Wenna , Evans Lauren , Wu Yiyuan , Kim Min-hyung , Ghosh Arnab , Alexopoulos George , Pathak Jyotishman , Banerjee Samprit 
Published: October 2024

Abstract

Background: Late middle-aged adults with depression experience a high illness burden often resulting from multiple chronic conditions. Risk stratification using administrative claims data is a promising method to identify enrollees at highest risk of experiencing psychiatric hospitalization and emergency department (ED) utilization. The goal of this study was to develop and validate a non-proprietary model to identify late middle-aged adults with depression at risk of 1- and 2- year psychiatric hospitalization and ED utilization, with broad applicability to commercially insured adults.
Methods: We conducted a retrospective cohort study on 71,682 commercially insured adults aged 55-64 years with depression diagnosis. National health insurance claims were used to capture demographics, health care utilization, and health status during the baseline year. Health status was captured using 70 chronic health conditions, and 46 mental health conditions. The four outcomes were one-year and two-year psychiatric hospitalization and ED utilization.
Results: For 1-year risk of psychiatric hospitalization, the final model achieved an AUC of 0.806, with a sensitivity of ­­61% and a specificity of 85%. For 2-year risk of psychiatric hospitalization, the final model achieved an AUC of 0.781, with a sensitivity of 68% and a specificity of 75%. For 1-year risk of psychiatric ED utilization, the final model achieved an AUC of 0.727, with a sensitivity of 56% and specificity of 78%. For the 2-year risk of psychiatric ED utilization, the final model achieved an AUC of 0.748, with a sensitivity of 67% and a specificity of 72%. The final models for all four outcomes outperformed their corresponding models using only demographics and prior utilization.
Conclusions:Predictive modeling using diagnoses and other demographic characteristics readily available in claims data can be used to identify late middle-aged adults with depression at risk of psychiatric hospitalization and ED utilization. Given the aging of the U.S. population and the significant burden of illness experienced by those with depression and multiple chronic conditions, this approach may assist policy makers and health care administrators in the identification of high-risk patients who may benefit from additional screening and care management interventions.
Exercise Mitigates MPTP-Induced Mitochondrial Fragmentation through the Irisin/AMPK/SIRT1 Pathway
Authors: Li Nan , Wang Bin , Wang Yuanxin , Tian Xin , Lin Junjie , Sun Xun , Sun Yu , Zhang Xin , Xu Haocheng , Li Mingzhi , Zeng Fanxi , Zhao Renqing 
Published: October 2024

Abstract

Purpose
Mitochondrial dysfunction plays a crucial role in the pathogenesis of Parkinson’s disease (PD), yet therapeutic strategies targeting mitochondrial function remain limited. Exercise has shown neuroprotective benefits in PD, but the underlying mechanisms are not fully understood. This study aimed to investigate how exercise affects MPTP-induced excessive apoptosis and mitochondrial fission in PD mice, with a focus on the irisin/AMPK/SIRT1 pathway.
Methods
Thirty-two male C57BL/6J mice, aged 7–8 weeks, were randomly assigned to control (n = 8) and experimental groups (n = 24). Mice in the experimental groups were administered intraperitoneal injections of MPTP to induce the PD model. Subsequently, the experimental mice were divided into three groups (8 mice in each group): the sedentary group (PD), the group subjected to ten weeks of treadmill exercise (PDEX), and the group receiving both treadmill exercise and irisin antagonist injections (EXRG). Upon completion of the ten-week intervention, behavioral assessments were performed. Following this, the mice were euthanized to collect brain samples and subjected to immunohistochemistry, immunofluorescence, ELISA, and Western blot analyses.
Results
MPTP-treated mice exhibited significant motor dysfunction and dopaminergic neuron loss in the nigrostriatal regions, which were ameliorated by a 10-week exercise intervention. Exercise notably reduced MPTP-induced neuronal apoptosis, as evidenced by decreased cellular fragments and abnormal nuclear morphology, increased Bcl-2 protein levels, and decreased Bax expression. Additionally, exercise mitigated abnormal mitochondrial fission in PD mice, as shown by reduced immunohistochemistry and protein expression of Drp1, Fis1, and MFF. In the substantia nigra of PD mice, the expression levels of irisin, p-AMPK, and SIRT1 were decreased but were elevated following the 10-week exercise intervention. However, blocking the irisin signaling by chronic treatment with cyclo RGDyk potentially counteracted the exercise-induced elevations in p-AMPK and Sirt1 expression. Moreover, the beneficial effects of exercise on neuronal apoptosis and mitochondrial fission were reversed by blocking irisin signaling pathways.
Conclusion
These findings suggest that regular exercise is beneficial in alleviating motor dysfunction in MPTP-treated mice, partly achieved through the preservation of dopaminergic neurons, reduction of excessive neuronal apoptosis, and improvement of normal mitochondrial fission. The excise-associated neuroprotective effects are likely linked to the irisin/AMPK/Sirt1 signalling pathway.
Traumatic Brain Injury Exacerbates Alcohol Consumption and Neuroinflammation with Decline in Cognition and Cholinergic Activity
Authors: Wang Jun , Gangal Himanshu , Iannucci Jaclyn , Huang Yufei , Chen Ruifeng , Purvines William , Davis William , Rivera Arian , Johnson Giles , Xie Xueyi , Mukherjee Sanjib , Vierkant Valerie , Mims Kaley , Neill Katherine O' , Wang Xuehua , Shapiro Lee A. 
Published: October 2024

Abstract

Traumatic brain injury (TBI) is a global health challenge, responsible for 30% of injury-related deaths and significantly contributing to disability. Annually, over 50 million TBIs occur worldwide, with most adult patients at emergency departments showing alcohol in their system. TBI is also a known risk factor for alcohol abuse, yet its interaction with alcohol consumption remains poorly understood. In this study, we demonstrate that the fluid percussion injury (FPI) model of TBI in mice significantly increases alcohol consumption and impairs cognitive function. At cellular levels, FPI markedly reduced the number and activity of striatal cholinergic interneurons (CINs) while increasing microglial cells. Notably, depleting microglial cells provided neuroprotection, mitigating cholinergic loss and enhancing cholinergic activity. These findings suggest that TBI may promote alcohol consumption and impair cognitive abilities through microglia activation and consequently reduced cholinergic function. Our research provides critical insights into the mechanisms linking TBI with increased alcohol use and cognitive deficits, potentially guiding future therapeutic strategies.