bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for a role of the histone deacetylase SIRT6 in DNA damage response of multiple myeloma cells
Authors: Cea Michele , Cagnetta Antonia , Adamia Sophia , Acharya Chirag , Tai Yu-Tzu , Fulciniti Mariateresa , Ohguchi Hiroto , Munshi Aditya , Acharya Prakrati , Bhasin Manoj K. , Zhong Lei , Carrasco Ruben , Monacelli Fiammetta , Ballestrero Alberto , Richardson Paul , Gobbi Marco , Lemoli Roberto M. , Munshi Nikhil , Hideshima Teru , Nencioni Alessio , Chauhan Dharminder , Anderson Kenneth C. 
Published: December 2015

Key Points
SIRT6 is highly expressed in multiple myeloma cells and blocks expression of ERK-regulated genes. Targeting SIRT6 enzymatic activity sensitizes multiple myeloma cells to DNA-damaging agents.
Tissues from equine cadaver ligaments up to 72 hours of post-mortem: a promising reservoir of stem cells
Authors: Shikh Alsook Mohamad Khir , Gabriel Annick , Piret Joëlle , Waroux Olivier , Tonus Céline , Connan Delphine , Baise Etienne , Antoine Nadine 
Published: December 2015

Abstract
Background
Mesenchymal stem cells (MSCs) harvested from cadaveric tissues represent a promising approach for regenerative medicine. To date, no study has investigated whether viable MSCs could survive in cadaveric tissues from tendon or ligament up to 72 hours of post-mortem. The purpose of the present work was to find out if viable MSCs could survive in cadaveric tissues from adult equine ligaments up to 72 hours of post-mortem, and to assess their ability (i) to remain in an undifferentiated state and (ii) to divide and proliferate in the absence of any specific stimulus.

Methods
MSCs were isolated from equine cadaver (EC) suspensory ligaments within 48–72 hours of post-mortem. They were evaluated for viability, proliferation, capacity for tri-lineage differentiation, expression of cell surface markers (CD90, CD105, CD73, CD45), pluripotent transcription factor (OCT-4), stage-specific embryonic antigen-1 (SSEA-1), neuron-specific class III beta-tubulin (TUJ-1), and glial fibrillary acidic protein (GFAP). As well, they were characterized by transmission electron microscope (TEM).

Results
EC-MSCs were successfully isolated and maintained for 20 passages with high cell viability and proliferation. Phase contrast microscopy revealed that cells with fibroblast-like appearance were predominant in the culture. Differentiation assays proved that EC-MSCs are able to differentiate towards mesodermal lineages (osteogenic, adipogenic, chondrogenic). Flow cytometry analysis demonstrated that EC-MSCs expressed CD90, CD105, and CD73, while being negative for the leukocyte common antigen CD45. Immunofluorescence analysis showed a high percentage of positive cells for OCT-4 and SSEA-1. Surprisingly, in absence of any stimuli, some adherent cells closely resembling neuronal and glial morphology were also observed. Interestingly, our results revealed that approximately 15 % of the cell populations were TUJ-1 positive, whereas GFAP expression was detected in only a few cells. Furthermore, TEM analysis confirmed the stemness of EC-MSCs and identified some cells with a typical neuronal morphology.

Conclusions
Our findings raise the prospect that the tissues harvested from equine ligaments up to 72 hours of post-mortem represent an available reservoir of specific stem cells. EC-MSCs could be a promising alternative source for tissue engineering and stem cell therapy in equine medicine.
Maximizing Stochastic Monotone Submodular Functions
Authors: Asadpour Arash , Nazerzadeh Hamid 
Published: December 2015

We study the problem of maximizing a stochastic monotone submodular function with respect to a matroid constraint. Because of the presence of diminishing marginal values in real-world problems, our model can capture the effect of stochasticity in a wide range of applications. We show that the adaptivity gap—the ratio between the values of optimal adaptive and optimal nonadaptive policies—is bounded and is equal to e/(e − 1). We propose a polynomial-time nonadaptive policy that achieves this bound. We also present an adaptive myopic policy that obtains at least half of the optimal value. Furthermore, when the matroid is uniform, the myopic policy achieves the optimal approximation ratio of 1 − 1/e. This paper was accepted by Dimitris Bertsimas and Yinyu Ye, optimization.
Surgical mortality in patients more than 80 years of age
Authors: Khan-Kheil AM , Khan HN 
Published: December 2015

Introduction Patients aged >80 years account for a considerable proportion of the population admitted to hospital under general surgeons. We aimed to establish the prevalence of mortality in patients aged >80 years who underwent emergency general, vascular and urological surgery within a 13-month period at a large teaching hospital in the UK. Materials and methods A retrospective analysis was carried out of all patients aged ≥80 years admitted on acute on-call emergency under general, vascular or urological surgeons. Patient demographics (including comorbidities and sex) were recorded. American Society of Anesthesiologists scores were reviewed from anaesthetic records. The outcome measure was 30-day mortality for those who had undergone emergency general, vascular or urological surgery. Results A total of 4,069 patients were admitted under general, vascular and urological surgeons during the study period. Of these patients, 521 were aged >80 years. Sixty-three patients underwent emergency surgery and 12 died <30 days after surgery (mortality = 19%). The most common procedure was laparotomy (20 cases). The most common co-morbidity was cardiac disease, which included hypertension, ischaemic heart disease, and hypercholesterolemia. A considerable proportion of patients also had malignant disease and arthritis. Conclusions The present study suggests that emergency surgery should not be denied to subjects aged >80 years based on age alone. Mortality varies according to the type of emergency procedure. Mortality was highest after laparotomy and vascular surgery whereas, for more routine procedures such as hernia repair and abscess drainage, survival was almost 100% after 30 days.
Male anxiety, inadequacy and victimhood: Insecure and immature men in recent Norwegian cinema
Authors: Donnar Glen 
Published: December 2015

Abstract
This article examines white heterosexual men in five recent Norwegian films that expose them as anxious and immature. These films mirror wider trends in the evaluation of white male failure and inadequacy across western cinemas since the mid-2000s. Extending critical discourses on ‘crises of masculinity’, especially in US film, this article finds in these films the ambivalent legacy of enforced gender politics in Norway over the last four decades. More specifically, they focus on Norwegian male complaints and ambivalence towards societal developments in favour of gender equality. Male characters exploit the opportunities of sexual liberation. They simultaneously objectify and neurotically fear women. A multitude of mental and physical ‘ailments’ establishes the men as victims. In an attempt to reclaim destabilized masculinities, parents and sexualized women are demonized. Ultimately, however, the desire to contain female sexuality and the transition into (hetero)sexual maturity is frustrated, and male anxieties remain unrelieved and unresolved.
Authorial self-fashioning in Jörn Donner’s Portraits of Women
Authors: Koivunen Anu 
Published: December 2015

Abstract
In his films of the late 1960s and the early 1970s, Jörn Donner, a Swedish-speaking Finnish author, critic and film director, employed his body and popular media image as a contemporary celebrity for narrative and marketing purposes. Focusing on the film Naisenkuvia/Portraits of Women (1970), a metanarrative about the alliance of art cinema and pornography in the 1960s and a parody of Donner’s public persona, this article investigates Donner’s ‘authoring practices’ and gestures of authorial ‘self-projection’ amidst the mediatized sexual revolution of the 1960s. Portraits of Women reveals Donner’s appropriation and analysis of a newly sexualized public sphere, but the film also reads as a crisis point. While capitalizing on his public persona by casting himself as the male lead, Donner was forced to acknowledge that self-fashioning in the sphere of public sex escapes authorial control.
Assessment of calibration methods on impedance pneumography accuracy
Authors: Młyńczak Marcel , Niewiadomski Wiktor , Żyliński Marek , Cybulski Gerard 
Published: December 2015

AbstractThe aim was to assess accuracy of tidal volumes (TV) calculated by impedance pneumography (IP), reproducibility of calibration coefficients (CC) between IP and pneumotachometry (PNT), and their relationship with body posture, breathing rate and depth. Fourteen students performed three sessions of 18 series: normal and deep breathing at 6, 10, 15 breaths/min rates, while supine, sitting and standing; 18 CC were calculated for every session. Session 2 was performed 2 months after session 1, session 3 1–3 days after session 2. TV were calculated using full or limited set of CC from current session, in case of sessions 2 and 3 also using CC from session 1 and 2, respectively. When using full set of CC from current session, IP underestimated TV by -3.2%. Using CC from session 2 for session 3 measurements caused decrease of relative difference: -3.9%, from session 1 for session 2: -5.3%; for limited set of CC: -5.0%. The body posture had significant effect on CC. The highest accuracy was obtained when all factors influencing CC were considered. The application of CC related only to body posture may result in shortening of calibration and moderate accuracy loss. Using CC from previous session compromises accuracy moderately.
The Influence Of Variability Of Water Resources In Lowland Forests On Selected Parameters Describing The Condition Of Trees
Authors: Tyszka Jan , Stolarek Andrzej , Fronczak Ewa 
Published: December 2015

Abstract
The influence of water conditions on the condition and growth of tree stands has been analysed in the context of the climatic and hydrological functions forest plays. Long observational series obtained for precipitation, outflow and depths below the surface of the water table have been put together with measured increases in the breast-height diameters of Scots pines and the severity of crown defoliation observable in selected tree species growing on the Polish Lowland, in order to determine the overall scope to the reaction stand condition manifests in the face of ongoing variability of water conditions within forest. An overall improvement in the condition of stands over the last 20 years does not disguise several-year cyclicity to changes capable of shaping the situation, i.a. departures from long-term mean values for precipitation totals and groundwater levels. The condition of stands is seen to worsen in both dry and wet years. Analysis of the degree to which pine, spruce and broadleaved stands experience defoliation points to spruce stands responding most to extreme hydro-climatic conditions. Extreme situations as regards water resources were seen to involve a response over two-year time intervals in the case of coniferous stands. Unsurprisingly, optimal growing-season (June-September) precipitation totals correspond with long-term average figures, while being slightly higher for spruce (at 384 mm), than for Scots pine or broadleaved species (375 mm).
The relationships reported gain confirmation in analysis of periodic change in breast-height diameter increments characterising Scots pines, whose growth is seen to depend closely, not only on precipitation, but also above all on the depth of the water table in the summer half-year. Optimal depths of the water table proved to be different, being around 20 cm below ground in the case of marshy coniferous forest, 80 cm in wet habitats, and 135 cm in fresh habitats. Depending on the possibilities for water to soak into the rooting zone of trees there were even twofold differences in measured growth increments in Scots pine (as the dominant species in Poland’s lowland habitats).
The maintenance of stable water conditions (as the most variable environmental factor in forest) should be an overriding aim of management activity in this habitat. When account is taken of the influence of the state of water resources on biomass production, and then on the intensity of evapotranspiration and the absorption of carbon dioxide from the atmosphere, it is seen how important it is to achieve improvements in water conditions in forests, as such an important factor in combating climate change.
Climate Change – Is It Worse Than Expected?
Authors: Kundzewicz Zbigniew W. , Masson-Delmotte Valérie , Cubasch Ulrich , Skea Jim , Kleiber Michał 
Published: December 2015

Abstract
A review of findings contained in the IPCC AR5 Synthesis Report, of particular relevance to the Polish audience, is offered. Polish perspectives on coal-climate nexus are discussed in a broader, universal, context. Positive examples of climate policies in other countries are provided. The title of this paper refers to a public conference organized in Warsaw by the Embassies of France, Germany, and the UK and the Polish Academy of Sciences.
The Existential Democracy And Its Public Intellectual
Authors: Pralea Cristian 
Published: December 2015

Abstract
This paper is constructed as an elaborate answer to the question “what is and what should be a public intellectual today?” Using a range of critical theorists I argue that the function of a public intellectual is connected to specific conceptions of truth, and reveal the inherent difficulty of conceiving a straight-forward role of such a public intellectual within a relativist context. I then resolve the difficulty by elaborating an idea of an “existential democracy” revealed in and by the context of our inter-connected information society (cyberspace). What is a public intellectual today then? A public intellectual would have to be a genealogist, a critic of culture that would be capable of letting truth speak through writing, and, ultimately, a true practitioner and adherent of democracy understood existentially – meaning rather on an ontological level than political, or political only insofar as ontological. The paper also opens up a series of intriguing questions that are worth investigating in separate inquiries similar to the one I attempt here: what happens to the process of knowledge within the bounds of this existential democracy, what happens to our educational systems, and what happens with our already solidified academic understanding of humanistic research?
Crystal structures of 2-[5-nitrothien-2-yl)- CH=N–NR–CO(CH2)n]thiophene compounds (R = H or Me; n = 0 or 1)
Authors: Cardoso Laura N.F. , Noguiera Thais C.M. , Kaiser Carlos R. , Wardell James L. , Wardell Solange M.S.V. , de Souza Marcus V.N. 
Published: December 2015

AbstractThe crystal structures of four acylhydrazonyl derivatives of thiophene, 2-(ArCH=N–NHCO)- thiophene, (1a),2-(ArCH=N–NMeCO)-thiophene, (2a), 2-(ArCH=N–NHCOCH2)-thiophene, (3a) and 2-(ArCH=N–NMeCOCH2)-thiophene, (4a) [in each case Ar=5-nitrothien-2-yl] are reported. The molecular conformations of1aand2aare near planar, while those of3aand4aexhibit bends at the methylene carbon. Methylations at the hydrazonyl groups in1aand3a, to give2aand4a,do not result in any significant changes in the molecular conformations. Each of the four molecular conformations possesses aZC(O)NR/E(C=N)arrangement about the C(O)–NR–N=C(H, aryl) fragment (R=H or Me). The dominant intermolecular interactions in1aand3aare N–H···O(carbonyl) hydrogen bonds, with other intermolecular interactions being weaker interactions: C–H···O and N–O···π in1aand C–H···X (X=O, S, π) and π–π interactions in3a. In theN-methylated compounds, the intermolecular interactions are restricted to weaker C–H···O hydrogen bonds in2aand C–H···X (X=O or π) interactions in4a.
Cholinergic Neurotransmission in the Posterior Insular Cortex Is Altered in Preclinical Models of Neuropathic Pain: Key Role of Muscarinic M2 Receptors in Donepezil-Induced Antinociception
Authors: Ferrier Jérémy , Bayet-Robert Mathilde , Dalmann Romain , El Guerrab Abderrahim , Aissouni Youssef , Graveron-Demilly Danielle , Chalus Maryse , Pinguet Jérémy , Eschalier Alain , Richard Damien , Daulhac Laurence , Marchand Fabien , Balayssac David 
Published: December 2015

Neuropathic pain is one of the most debilitating pain conditions, yet no therapeutic strategy has been really effective for its treatment. Hence, a better understanding of its pathophysiological mechanisms is necessary to identify new pharmacological targets. Here, we report important metabolic variations in brain areas involved in pain processing in a rat model of oxaliplatin-induced neuropathy using HRMAS1H-NMR spectroscopy. An increased concentration of choline has been evidenced in the posterior insular cortex (pIC) of neuropathic animal, which was significantly correlated with animals' pain thresholds. The screening of 34 genes mRNA involved in the pIC cholinergic system showed an increased expression of the high-affinity choline transporter and especially the muscarinic M2 receptors, which was confirmed by Western blot analysis in oxaliplatin-treated rats and the spared nerve injury model (SNI). Furthermore, pharmacological activation of M2 receptors in the pIC using oxotremorine completely reversed oxaliplatin-induced mechanical allodynia. Consistently, systemic treatment with donepezil, a centrally active acetylcholinesterase inhibitor, prevented and reversed oxaliplatin-induced cold and mechanical allodynia as well as social interaction impairment. Intracerebral microdialysis revealed a lower level of acetylcholine in the pIC of oxaliplatin-treated rats, which was significantly increased by donepezil. Finally, the analgesic effect of donepezil was markedly reduced by a microinjection of the M2 antagonist, methoctramine, within the pIC, in both oxaliplatin-treated rats and spared nerve injury rats. These findings highlight the crucial role of cortical cholinergic neurotransmission as a critical mechanism of neuropathic pain, and suggest that targeting insular M2 receptors using central cholinomimetics could be used for neuropathic pain treatment.SIGNIFICANCE STATEMENTOur study describes a decrease in cholinergic neurotransmission in the posterior insular cortex in neuropathic pain condition and the involvement of M2 receptors. Targeting these cortical muscarinic M2 receptors using central cholinomimetics could be an effective therapy for neuropathic pain treatment.
miR-155-3p Drives the Development of Autoimmune Demyelination by Regulation of Heat Shock Protein 40
Authors: Mycko Marcin P. , Cichalewska Maria , Cwiklinska Hanna , Selmaj Krzysztof W. 
Published: December 2015

microRNA-155 (miR-155) plays an important role in posttranscriptional gene regulation of the immune system. We and others have described miR-155 upregulation in T helper cells (Th) during the development of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. We have shown that mice in which the miR-155 host gene (MIR155HG) has been deactivated are resistant to EAE. MIR155HG produces two different miRNA strands, miR-155-5p and miR-155-3p, and miR-155-5p has been considered the only functional miR-155 form. Surprisingly, we found that miR-155-3p is also strongly upregulated in Th cells infiltrating the brain in EAE. Functional manipulation of miR-155-3p expression revealed its particular role in regulation of Th17 development. The search for miRNA-155–3p target genes highlighted transcripts of two heat shock protein 40 genes,Dnaja2andDnajb1. These two genes negatively regulated Th17 differentiation, leading to decreased EAE. Therefore, our findings provide new insights into a previously unknown mechanism by which miR-155-3p controls Th17 cell differentiation and autoimmune demyelination.SIGNIFICANCE STATEMENTMultiple sclerosis (MS) is brain-specific autoimmune disease mediated by T helper (Th) cells autoreactive to myelin. The mechanisms leading to MS are not fully understood and microRNAs (miRNAs) emerge as important regulators of the process. We report that, in an MS murine model of experimental autoimmune encephalomyelitis, miR-155 controls Th cell function by an unusual mechanism involving a rare form, miR-155-3p. miR-155-3p is specifically found in brain-infiltrating myelin-autoreactive CD4+T cells and contributes to the development of an encephalitogenic Th17 population. miR-155-3p promotes Th17 by inhibiting two heat shock protein 40 genes,Dnaja2andDnajb1. Our findings indicate a unique miRNA function in the brain-infiltrating Th cells and suggestDnaja2andDnajb1as targets for intervention in autoimmune demyelination.
Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins
Authors: Mehta Suresh L. , Kim TaeHee , Vemuganti Raghu 
Published: December 2015

Ischemia induces extensive temporal changes in cerebral transcriptome that influences the neurologic outcome after stroke. In addition to protein-coding RNAs, many classes of noncoding RNAs, including long noncoding RNAs (LncRNAs), also undergo changes in the poststroke brain. We currently evaluated the functional significance of an LncRNA called Fos downstream transcript (FosDT) that is cogenic withFosgene. Following transient middle cerebral artery occlusion (MCAO) in adult rats, expression ofFosDTandFoswas induced. FosDT knockdown significantly ameliorated the postischemic motor deficits and reduced the infarct volume. Focal ischemia also increased FosDT binding to chromatin-modifying proteins (CMPs) Sin3a and coREST (corepressors of the transcription factor REST). Furthermore, FosDT knockdown derepressed REST-downstream genesGRIA2,NFκB2, andGRIN1in the postischemic brain. Thus, FosDT induction and its interactions with REST-associated CMPs, and the resulting regulation of REST-downstream genes might modulate ischemic brain damage. LncRNAs, such as FosDT, can be therapeutically targeted to minimize poststroke brain damage.SIGNIFICANCE STATEMENTMammalian brain is abundantly enriched with long noncoding RNAs (LncRNAs). Functional roles of LncRNAs in normal and pathological states are not yet understood. This study identified that LncRNA FosDT induced after transient focal ischemia modulates poststroke behavioral deficits and brain damage. These effects of FosDT in part are due to its interactions with chromatin-modifying proteins Sin3a and coREST (corepressors of the transcription factor REST) and subsequent derepression of REST-downstream genesGRIA2,NFκB2, andGRIN1. Therefore, LncRNA-mediated epigenetic remodeling could determine stroke outcome.