bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perception of nursing homes as accomodation units<p>Konaklama birimi olarak huzurevi algısı
Authors: Aktaş Polat Semra , Hira İsmail 
Published: October 2017

The purpose of this study is to determine how nursing homes are perceived as accomodation units by the age of 60 and above elderly people living at home and whether there is a relationship between these people’s perceptions and their gender, the cities they live and the people they live with. Research area includes Sinop, Kastamonu and Çankırı cities located in the TR82 region in Turkey. Research data were collected through face to face questionnaires from 400 elderly people aged 60 and over residing in the TR82 region. The data were analyzed by frequency analysis and the chi-square (x2) test.According to the results a nursing home is perceived as reassuring units besides the feel of abandonment, not to be a burden to anyone, a place for the lonely and poor old, reducing future anxiety for the old, a place for helpless people to stay, a result of loneliness in a sense that might be a solution to some problems.According to chi-square (x2) test results it is determined that although there is no relationship between perception of nursing homes and gender, there is a statistically significant relationship between some phrases related to perception of nursing homes and inhabited city and people the old live with. Extended English abstract is in the end of PDF (TURKISH) file. ÖzetBu çalışmanın amacı, bir konaklama birimi olarak huzurevlerinin, 60 yaş ve üzeri evde yaşayan yaşlılar tarafından nasıl algılandığını ve bu yaşlıların, huzurevlerine yönelik düşünceleri ile cinsiyetleri, ikamet ettikleri şehirler ve birlikte yaşadıkları kişiler arasında bir ilişkinin olup olmadığını tespit etmektir. Araştırma alanı Sinop, Kastamonu ve Çankırı’yı içeren TR82 Bölgesi’dir. Araştırma verileri, 60 yaş ve üzeri 400 yaşlıdan yüz yüze anket tekniği ile toplanmış, frekans analizi ve ki-kare (x2) testi ile analiz edilmiştir.Araştırma sonuçlarına göre huzurevleri; terk edilmişliği hissettiren, kimseye yük olmamayı sağlayan, kimsesiz ve yoksul yaşlıların kaldığı bir yer, yaşlıların gelecek kaygılarını azaltan, çaresiz insanların kalmayı tercih ettiği, bir anlamda da yalnızlığın bir sonucu, bazı sorunlara bir çözüm olabilecek, güven veren birimler olarak algılanmaktadır.Yapılan ki-kare (x2) testi sonucuna göre; huzurevleri algısı ile cinsiyet arasında bir ilişki olmamasına rağmen, huzurevi algısına yönelik bazı ifadeler ile ikamet edilen şehir ve yaşlının beraberinde yaşadığı kişiler arasında istatistiksel açıdan anlamlı bir ilişki tespit edilmiştir.
Intragenic hypomethylation of DNMT3A in patients with myelodysplastic syndrome
Authors: Zhang Ying-Ying , Zhou Jing-Dong , Yang Dong-Qin , He Pin-Fang , Yao Dong-Ming , Qian Zhen , Yang Jing , Xu Wen-Rong , Lin Jiang , Qian Jun 
Published: October 2017

Abstract

Background:

DNMT3A is a DNA methyltransferase that acts in de novo methylation. Aberrant expression of DNMT3A has been reported in several human diseases, including myelodysplastic syndrome (MDS). However, the pattern of DNMT3A methylation remains unknown in MDS.

Methods:
The present study was aimed to investigate the methylation status of DNMT3A intragenic differentially methylated region 2 (DMR2) using real-time quantitative methylation-specific PCR and analyze its clinical significance in MDS.

Results:
Aberrant hypomethylation of DNMT3A was found in 57% (51/90) MDS cases. There were no significant differences in age, sex, white blood cell counts, platelet counts, hemoglobin counts and World Health Organization, International Prognostic Scoring System and karyotype classifications between DNMT3A hypomethylated and DNMT3A hypermethylated groups. However, the patients with DNMT3A hypomethylation had shorter overall survival time than those without DNMT3A hypomethylation (11 months vs. 36 months, p=0.033). Multivariate analysis confirmed the independent adverse impact of DNMT3A hypomethylation in MDS.

Conclusions:
Our data suggest that DNMT3A DMR2 hypomethylation may be a negative prognostic hallmark in MDS.
The venous thromboembolic risk and the clot wave analysis: a useful relationship?
Authors: Ruberto Maria Filomena , Marongiu Francesco , Mandas Antonella , Mameli Antonella , Porru Mariagrazia , Cianchetti Elisabetta , Barcellona Doris 
Published: October 2017

Abstract

Background:
Hospitalized patients with acute medical conditions have higher venous thromboembolism (VTE) risk. A patient with a final Padua Prediction Score (PPS) of ≥4 is considered to be at high risk for VTE. The aim of this study was to investigate on a possible relationship between PPS, the dynamics of the clot formation, i.e. the clot waveform analysis (CWA) of aPTT, fibrinogen and D-Dimer in a large group of medical patients.

Methods:
CWA in terms of velocity (first derivative), acceleration (second derivative), density (Delta) of aPTT, fibrinogen, D-Dimer and PPS for VTE were determined in 801 medical patients divided in three groups (without antithrombotic prophylaxis and high PPS, without antithrombotic prophylaxis and low PPS, with antithrombotic prophylaxis and high PPS) and a group of healthy subjects.

Results:
CWA, fibrinogen and D-Dimer values were higher in the medical patients with high PPS with or without antithrombotic prophylaxis when compared with patients without antithrombotic prophylaxis with low PPS and healthy subjects. The second derivative, fibrinogen and D-Dimer were significantly associated with a high PPS score (≥4): odds ratio (OR) = 1.53, 95% confidence interval (CI) = 1.03–2.28; OR = 1.91, 95% CI = 1.3–2.79; OR = 3.16, 95% CI = 2.29–4.36, respectively. Interactions between first derivative and D-Dimer (OR = 2.14, 95% CI = 1.23–3.72) and first derivative and fibrinogen (OR = 1.75, 95% CI = 1.02–2.98) were found.

Conclusions:
CWA could give useful information to recognize a hypercoagulable state in patients admitted to a medical ward with high and low PPS. First and second derivative aPTT, D-Dimer and fibrinogen levels could be added to PPS to better assess the global thromboembolic risk of these patients.
How to reduce scientific irreproducibility: the 5-year reflection
Authors: Fiala Clare , Diamandis Eleftherios P. 
Published: October 2017

AbstractWe discuss in depth six causes of scientific irreproducibility and their ramifications for the clinical sciences: fraud, unfounded papers published by prominent authorities, bias, technical deficiencies, fragmented science and problems with big data. Some proposed methods to combat this problem are briefly described, including an effort to replicate results from some high impact papers and a proposal that authors include detailed preclinical data in papers with supposedly high translational value. We here advocate for a 5-year reflection on papers with seemingly high clinical/translational potential, published alongside the original paper where authors reflect on the quality, reproducibility and impact of their findings. These reflections can be used as a benchmark for credibility, and begin a virtuous cycle of improving the quality of published findings in the literature.
Strategies to define performance specifications in laboratory medicine: 3 years on from the Milan Strategic Conference
Authors: Panteghini Mauro , Ceriotti Ferruccio , Jones Graham , Oosterhuis Wytze , Plebani Mario , Sandberg Sverre 
Published: October 2017

AbstractMeasurements in clinical laboratories produce results needed in the diagnosis and monitoring of patients. These results are always characterized by some uncertainty. What quality is needed and what measurement errors can be tolerated without jeopardizing patient safety should therefore be defined and specified for each analyte having clinical use. When these specifications are defined, the total examination process will be “fit for purpose” and the laboratory professionals should then set up rules to control the measuring systems to ensure they perform within specifications. The laboratory community has used different models to set performance specifications (PS). Recently, it was felt that there was a need to revisit different models and, at the same time, to emphasize the presuppositions for using the different models. Therefore, in 2014 the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) organized a Strategic Conference in Milan. It was felt that there was a need for more detailed discussions on, for instance, PS for EQAS, which measurands should use which models to set PS and how to set PS for the extra-analytical phases. There was also a need to critically evaluate the quality of data on biological variation studies and further discussing the use of the total error (TE) concept. Consequently, EFLM established five Task Finish Groups (TFGs) to address each of these topics. The TFGs are finishing their activity on 2017 and the content of this paper includes deliverables from these groups.
(Non) Compliance with the International Health Regulations of the WHO from the Perspective of the Law of International Responsibility
Authors: Spagnolo Andrea 
Published: October 2017

Abstract
International Health Regulations (IHR) of the World Health Organization (WHO) are fundamental to ensure an adequate response of the international community to health emergencies such as the spread of the virus Ebola in 2014. This notwithstanding, WHO's Member States appear reluctant to comply with the IHR and in particular they violate the ban on unnecessary trade and travel restrictions. After having presented the lack of compliance with the IHR, the present article analyses the means at the disposal of the WHO for sanctioning the behaviour of its Member States, both from the perspective of WHO's internal rules and from the perspective of the law of international responsibility, evaluating if countermeasures might represent a viable solution. The conclusions will offer a broad reflection on the codification process of the rules on the responsibility of international organisations concluded by the International Law Commission in 2011.
Fetal analysis with invasive method (FA-I) and fetal analysis with non-invasive method (FA-NI): replacing current, deceptively imprecise clinical nomenclature
Authors: Chervenak Frank A. , McCullough Laurence B. , Dudenhausen Joachim 
Published: October 2017

AbstractThere is a problem with the current nomenclature of prenatal evaluation. The current nomenclature of “prenatal testing” and “prenatal screening” – along with their subsets of “ultrasound testing,” “ultrasound screening,” “non-invasive prenatal testing,” “non-invasive prenatal screening,” and “prenatal diagnosis” – has become so imprecise that clinical misinterpretation and distortion of the informed consent process are increasingly difficult to avoid. To remedy this problem, we propose a new, precise nomenclature: “fetal analysis with invasive method” (FA-I) and “fetal analysis with non-invasive method,” (FANI) using various techniques. This new nomenclature is designed to be precise and therefore facilitate effective communication among physicians and with pregnant women. For ease of use the new nomenclature can be formulated as an abbreviation: FA-I and FA-NI.
Molecular architecture of pterin deaminase from Saccharomyces cerevisiae NCIM 3458
Authors: Murugesan Thandeeswaran , Velliayadevar Karuppuswamy , Easwaran Murugesh , KG Kiran , KA Ayub Nawaz , Ramasamy Mahendran , Muthusamy Palaniswamy , Jayaraman Angayarkanni 
Published: October 2017

Abstract
As early as 1974, reports have confirmed the anticancer activity of pterin deaminase isolated from fungi. The enzyme has also been reported in bacteria, fungi and slime mold genera, but the enzyme characterization was effetely done. The present study attempted to purify and characterize pterin deaminase enzyme from Saccharomyces cerevisiae NCIM 3458. The protein was extracted from the extracellular extract by using the ethanol precipitation method. Partial purification of pterin deaminase enzyme was achieved by ion exchange chromatography (Hi-Trap QFF) by fast protein liquid chromatography (AKTA purifier). The molecular weight of the protein was apparently determined by SDS-PAGE, and the presence of pterin deaminase was confirmed by activity staining. The purified enzyme was further biochemically characterized. Molecular docking studies showed higher binding affinity towards folic acid interaction. The structural characterization of this protein may open the windows for new drug targets for cancer therapy.
Effect of chemical treatment on thermal properties of hair fiber-based reinforcement of HF/HDPE composites
Authors: Srivastava Prashant , Sinha Shishir 
Published: October 2017

Abstract
The present study deals with the effects of fiber loading and alkali treatment (AT) on thermal behavior of a hair fiber (HF)-reinforced high-density polyethylene (HDPE) composite. The HF/HDPE-reinforced polymer composite has been prepared through a compression molding technique, which provided optimum thermal stability at 15 wt% of the fiber in the reinforced composite. The thermal stability of the composite has been investigated using a thermal analyzer [thermogravimetric analysis (TGA), derivative thermogravimetric analysis (DTG) and differential scanning calorimetry (DSC)]. The ATs of HF-reinforced composites have affected the thermal stability of the material, in which the observed optimum thermal stability is 0.25 N AT 15% HF/HDPE-reinforced composites. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and tensile test played an important role in the study of the thermal stability of the material. FTIR analysis was used to investigate the chemical groups between the fiber and matrix. The morphology of the fiber is beneficial for the study of the surface treatment effect on the HF. The tensile test examined the optimum strength at 0.25 N AT 15% HF/HDPE composite, and a good relationship between the thermal and mechanical properties was also observed.
Qualification of a Small Gas Turbine Engine as a Starter Unit
Authors: Mishra R. K. , Kumar Prashant , Jayasihma K. S. , Mistry S. N. 
Published: October 2017

Abstract
The certification philosophy plays an important role in ensuring the airworthiness qualification of a small gas turbine engine designed as a starter unit. The small and compactness of the engine, high rotational speed of the rotors, requirements of torque and starting capability at sea level to high altitude airfields and consecutive starts within stipulated time impose a great challenge from airworthiness point of view. This paper presents the methodology adopted and various stages of qualification, standards followed and results based on which the starter unit has been qualified for fitment on the designated aircraft.
Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins
Authors: Smith Katharine R. , Jones Kelly A. , Kopeikina Katherine J. , Burette Alain C. , Copits Bryan A. , Yoon Sehyoun , Forrest Marc P. , Fawcett-Patel Jessica M. , Hanley Jonathan G. , Weinberg Richard J. , Swanson Geoffrey T. , Penzes Peter 
Published: October 2017

Appropriate excitatory/inhibitory (E/I) balance is essential for normal cortical function and is altered in some psychiatric disorders, including autism spectrum disorders (ASDs). Cell-autonomous molecular mechanisms that control the balance of excitatory and inhibitory synapse function remain poorly understood; no proteins that regulate excitatory and inhibitory synapse strength in a coordinated reciprocal manner have been identified. Using super-resolution imaging, electrophysiology, and molecular manipulations, we show that cadherin-10, encoded byCDH10within the ASD risk locus 5p14.1, maintains both excitatory and inhibitory synaptic scaffold structure in cultured cortical neurons from rats of both sexes. Cadherin-10 localizes to both excitatory and inhibitory synapses in neocortex, where it is organized into nanoscale puncta that influence the size of their associated PSDs. Knockdown of cadherin-10 reduces excitatory but increases inhibitory synapse size and strength, altering the E/I ratio in cortical neurons. Furthermore, cadherin-10 exhibits differential participation in complexes with PSD-95 and gephyrin, which may underlie its role in maintaining the E/I ratio. Our data provide a new mechanism whereby a protein encoded by a common ASD risk factor controls E/I ratios by regulating excitatory and inhibitory synapses in opposing directions.SIGNIFICANCE STATEMENTThe correct balance between excitatory/inhibitory (E/I) is crucial for normal brain function and is altered in psychiatric disorders such as autism. However, the molecular mechanisms that underlie this balance remain elusive. To address this, we studied cadherin-10, an adhesion protein that is genetically linked to autism and understudied at the cellular level. Using a combination of advanced microscopy techniques and electrophysiology, we show that cadherin-10 forms nanoscale puncta at excitatory and inhibitory synapses, maintains excitatory and inhibitory synaptic structure, and is essential for maintaining the correct balance between excitation and inhibition in neuronal dendrites. These findings reveal a new mechanism by which E/I balance is controlled in neurons and may bear relevance to synaptic dysfunction in autism.
Dysregulation of the Mitochondrial Unfolded Protein Response Induces Non-Apoptotic Dopaminergic Neurodegeneration inC. elegansModels of Parkinson's Disease
Authors: Martinez Bryan A. , Petersen Daniel A. , Gaeta Anthony L. , Stanley Samuel P. , Caldwell Guy A. , Caldwell Kim A. 
Published: October 2017

Due to environmental insult or innate genetic deficiency, protein folding environments of the mitochondrial matrix are prone to dysregulation, prompting the activation of a specific organellar stress-response mechanism, the mitochondrial unfolded protein response (UPRMT). InCaenorhabditis elegans, mitochondrial damage leads to nuclear translocation of the ATFS-1 transcription factor to activate the UPRMT. After short-term acute stress has been mitigated, the UPRMTis eventually suppressed to restore homeostasis toC. eleganshermaphrodites. In contrast, and reflective of the more chronic nature of progressive neurodegenerative disorders such as Parkinson's disease (PD), here, we report the consequences of prolonged, cell-autonomous activation of the UPRMTinC. elegansdopaminergic neurons. We reveal that neuronal function and integrity decline rapidly with age, culminating in activity-dependent, non-apoptotic cell death. In a PD-like context wherein transgenic nematodes express the Lewy body constituent protein α-synuclein (αS), we not only find that this protein and its PD-associated disease variants have the capacity to induce the UPRMT, but also that coexpression of αS and ATFS-1-associated dysregulation of the UPRMTsynergistically potentiate dopaminergic neurotoxicity. This genetic interaction is in parallel to mitophagic pathways dependent on theC. elegans PINK1homolog, which is necessary for cellular resistance to chronic malfunction of the UPRMT. Given the increasingly recognized role of mitochondrial quality control in neurodegenerative diseases, these studies illustrate, for the first time, an insidious aspect of mitochondrial signaling in which the UPRMTpathway, under disease-associated, context-specific dysregulation, exacerbates disruption of dopaminergic neuronsin vivo, resulting in the neurodegeneration characteristic of PD.SIGNIFICANCE STATEMENTDisruptions or alterations in the activation of pathways that regulate mitochondrial quality control have been linked to neurodegenerative diseases due in part to the central role of mitochondria in metabolism, ROS regulation, and proteostasis. The extent to which these pathways, including the mitochondrial unfolded protein response (UPRMT) and mitophagy, are active may predict severity and progression of these disorders, as well as sensitivity to compounding stressors. Furthermore, therapeutic strategies that aim to induce these pathways may benefit from increased study into cellular responses that arise from long-term or ectopic stimulation, especially in neuronal compartments. By demonstrating the detrimental consequences of prolonged cellular activation of the UPRMT, we provide evidence that this pathway is not a universally beneficial mechanism because dysregulation has neurotoxic consequences.
Early sex-dependent differences in response to environmental stress
Authors: Pérez-Cerezales Serafin , Ramos-Ibeas Priscila , Rizos Dimitrios , Lonergan Pat , Bermejo-Alvarez Pablo , Gutierrez-Adan Alfonso 
Published: October 2017

Greek:ΑΒΓΔΕΖΗΘΙΚΛΜΝΞΟΠΡΣΤΥΦΧΨΩαβγδεζηθικλμνξοπρςστυφχψωSpecial:¡〉〈♂♀•○▽△□■⇒⇐↕↔↓→↑←⅓™€…‡†”“’‘‖—–¿¾½¼»¶®«©§¥£¢Math:+│⊥⊙⊇⊆≧≦≥≤≡≠≒≈≅∽∼∴∮∬∫∥∠∞∝√∗−∑∏∉∈∇∂ÅΩ″′‰÷×·±°¬=Latin:ÀŸšŠœŒěĚčČċćĆăĂāÿýüûúùøöõôóòñïîíìëêéèçæåäãâáàÝÜÛÚÙØÖÕÔÓÒÑÏÎÍÌËÊÉÈÇÆÅÄÃÂÁDevelopmental plasticity enables the appearance of long-term effects in offspring caused by exposure to environmental stressors during embryonic and foetal life. These long-term effects can be traced to pre- and post-implantation development, and in both casesthe effects are usually sex-specific. During preimplantation development, male and female embryos exhibit an extensive transcriptional dimorphism mainly driven by incomplete X-chromosome inactivation. These early developmental stages are crucial for theestablishment of epigenetic marks that will be conserved throughout development, making it a particularly susceptible period for the appearance of long-term epigenetic-based phenotypes. Later in development, gonadal formation generates hormonal differencesbetween the sexes, and male and female placentae exhibit different responses to environmental stressors. The maternal environment, including hormones and environmental insults during pregnancy, contributes to sex-specific placental development that controls geneticand epigenetic programming during foetal development, regulating sex-specific differences, including sex-specific epigenetic responses to environmental hazards, leading to long-term effects. This review summarizes several human and animal studies examining sex-specific responses to environmental stressors during both the periconception period (caused by differences in sex chromosome dosage) and placental development (caused by both sex chromosomes and hormones). The identification of relevant sex-dependent trajectories caused by sex-chromosomes and/or sex-hormones is essential to define diagnostic markers and prevention/intervention protocols.
APC/CFZR-1 Controls SAS-5 Levels To Regulate Centrosome Duplication inCaenorhabditis elegans
Authors: Medley Jeffrey C , DeMeyer Lauren E , Kabara Megan M , Song Mi Hye 
Published: October 2017

AbstractAs the primary microtubule-organizing center, centrosomes play a key role in establishing mitotic bipolar spindles that secure correct transmission of genomic content. For the fidelity of cell division, centrosome number must be strictly controlled by duplicating only once per cell cycle. Proper levels of centrosome proteins are shown to be critical for normal centrosome number and function. Overexpressing core centrosome factors leads to extra centrosomes, while depleting these factors results in centrosome duplication failure. In this regard, protein turnover by the ubiquitin-proteasome system provides a vital mechanism for the regulation of centrosome protein levels. Here, we report that FZR-1, the Caenorhabditis elegans homolog of Cdh1/Hct1/Fzr, a coactivator of the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, functions as a negative regulator of centrosome duplication in the C. elegans embryo. During mitotic cell division in the early embryo, FZR-1 is associated with centrosomes and enriched at nuclei. Loss of fzr-1 function restores centrosome duplication and embryonic viability to the hypomorphic zyg-1(it25) mutant, in part, through elevated levels of SAS-5 at centrosomes. Our data suggest that the APC/CFZR-1 regulates SAS-5 levels by directly recognizing the conserved KEN-box motif, contributing to proper centrosome duplication. Together, our work shows that FZR-1 plays a conserved role in regulating centrosome duplication in C. elegans.