bluuebunny/crossref_metadata_2025_split huggingface dataset:
A review of research on computer‐aided translation technologies and their applications to assist learning and instruction
Authors: Shadiev Rustam , Chen Xuan , Altinay Fahriye 
Published: October 2024

AbstractBackgroundOur study analysed 46 articles on computer‐aided translation (CAT) and its educational uses. We explored two main areas: CAT technologies in learning and teaching, and the research methodologies of these studies.ObjectivesOur review covered the types of CAT technologies used, their applications in different learning settings, theoretical backgrounds and their pros and cons. We also looked at the research designs, participant demographics and data collection methods used in these studies.MethodsWe conducted an extensive search across academic databases using relevant keywords and employed open coding to analyse and categorize the content of identified articles, focusing on aspects related to CAT technologies in educational contexts.Results and ConclusionsOur findings showed the use of 22 distinct CAT technologies, with Google Translate being the most common. The studies mostly used empirical research methods and questionnaires. University students were the primary participants. CAT technologies were applied in various contexts, including language learning, translation teaching, cross‐cultural learning and foreign language lectures. We identified five key theoretical foundations for CAT use. Benefits included bridging language barriers, vocabulary enhancement, and grammar improvement as well as providing reference and feedback for writing and translation. Challenges included inaccuracies and inappropriate translations. However, most studies showed a positive impact of CAT on learning outcomes. Based on our analysis, we offer recommendations for educators and researchers in the field. This review provides a thorough understanding of CAT's applications and effects in education, guiding future advancements in this area.
Exploring students' computer‐supported collaborative argumentation with socio‐scientific issues
Authors: Chen Wenli , Han Yiting , Tan Jesmine , Chai Aileen Siew Cheng , Lyu Qianru , Lyna  
Published: October 2024

AbstractBackgroundThis study examined the effect of computer‐supported collaborative argumentation (CSCA) on secondary school students' understanding of socio‐scientific issues (SSI). Engaging students in collaborative argumentation is known to help with deepening their understanding of SSI.MethodsIn this study, a mixed‐method design is used to investigate 84 students' collaborative argumentation processes and outcomes. The statistical analysis, epistemic network analysis and qualitative uptake analysis results showed that CSCA was effective in supporting secondary school students' evidence‐based argumentation skills on SSI.Findings and ConclusionSeveral cases were presented to show how students engaged in CSCA to explore meaningful learning opportunities and how CSCA helped students' learning on SSI.ImplicationsThe findings provided insights for future innovative teaching and learning SSI in authentic classroom settings.
First Report of Leaf Blight of Blood Lily (Scadoxus multiflorus) Caused by Lasiodiplodia theobromae in India
Authors: Varma Penumatsa Kishore , Chandrasekhar Vanapalli , Supriya Puram , Vamsi Krishna Gokarla , Vasanthi V. , Janaki Prasad Ambatipudi , Bavana Keerthi Athmakuri , Manjula Ramappa , Chetan Kadavigere Kumar 
Published: October 2024

ABSTRACTLeaf blight was detected on potted blood lily (Scadoxus multiflorus) plants in a garden located in Andhra Pradesh, India. Pycnidia resembling a Lasiodiplodia species were consistently detected on the foliage of symptomatic plants. Based on morphology, cultural features and molecular analysis, the species was identified as Lasiodiplodia theobromae. Koch's postulates were fulfilled by pathogenicity tests carried out on potted blood lily plants. To the best of our knowledge, this is the first report of the occurrence of a disease caused by L. theobromae on S. multiflorus.
Content in nine minerals and seven vitamins of rotifers (Brachionus plicatilis) fed commercial diets and two forms of Nannochloropsis oculata
Authors: Eryalçın Kamil Mert , Dominguez David , Izquierdo Marisol 
Published: October 2024

AbstractThe objective of the present study was to determine the effects of spray‐dried and fresh forms of the microalgae Nannochloropsis oculata and other commonly used commercial diets on vitamin and mineral compositions of rotifer produced under commercial conditions. Experimental rotifers groups were fed Protein‐Plus® (PP treatment), Inactive Beaker's Yeast® (INBY treatment), Algome® (Algome treatment), freshly cultured N. oculata (FA treatment), and spray‐dried N. oculata (SDN treatment). At the end of the experiment, seven essential vitamins, nine macro‐ and micro‐minerals, and two pigments were analyzed in diets and rotifers. All the treatments tested provided adequate levels of Ca, P, Mg, and Co to the rotifers, but insufficient levels of I, Zn, and vitamins B2 and E. Moreover, PP diet produced rotifers with high levels of vitamins B1, B3 and B9, and P; the SDN diet produced rotifers with high levels of P and Cu; the Algome diet produced rotifers high in vitamins B3 and B9, and Ca; the INBY diet produced rotifers with high levels of vitamin B1 and Ca; and the FA diet produced rotifers with more vitamin B6, Se, and Cu. In conclusion, specific minerals or vitamins corrections are required in these feeds for rotifers to adequately meet the nutritional needs for marine larvae.
Energetically costly weaponry in the large morph of male stag beetles
Authors: Chen H. , Huang S.‐P. , Lin C.‐P. , Chen Z.‐Y. , Hsu Y. 
Published: October 2024

AbstractMales of many species develop disproportionately large weapons, and frequently the weapon size increases more quickly with body size in small than in large males—a trend often considered to result from the depletion of developmental resources limiting male weapon exaggeration. Based on the cost‐minimization hypothesis, a shallower weapon‐body size allometric slope in large than in small males could be selected for if the energetic maintenance costs of having oversized weapons increase faster in large than in small males. Whether males of different size groups that differ in the weapon‐body size allometric relationship also differ in the energetic costs of maintaining their weapons has not yet been investigated. Consequently, we studied this using the stag beetle (Cyclommatus mniszechi), in which males are larger in size and have larger mandibles than do females and are divided into two morphs: majors are larger and have longer mandibles than minors. In both male morphs, mandible size increases disproportionately with their body size, but it increases more quickly in minors than in majors. We measured the standard metabolic rate (SMR) of the three types of these beetles (the two male morphs and females) to investigate whether the energetic maintenance cost of carrying weapons differs among them. Body weight explained 80% of the variation in the SMR in the three types of beetles, and SMR increased more quickly with body weight in major males than in minor males or females. Weapon and body size also jointly explained approximately 80% of the variation in the SMR. Whereas SMR increased with body size in all three types of beetles (with a higher rate of increase in major males than in minor males or females), only the major males' SMR increased with weapon size. Overall, being heavier and larger and carrying oversized weapons are energetically costly in major males, something which could constrain the exaggeration of their weapons.
Tetraspanin 5 orchestrates resilience to salt stress through the regulation of ion and reactive oxygen species homeostasis in rice
Authors: Mani Balaji , Kaur Inderjit , Dhingra Yashika , Saxena Vidisha , Krishna G K , Kumar Rahul , Chinnusamy Viswanathan , Agarwal Manu , Katiyar‐Agarwal Surekha 
Published: October 2024

SummaryTetraspanins (TETs) are integral membrane proteins, characterized by four transmembrane domains and a unique signature motif in their large extracellular loop. They form dynamic supramolecular complexes called tetraspanin‐enriched microdomains (TEMs), through interactions with partner proteins. In plants, TETs are involved in development, reproduction and immune responses, but their role in defining abiotic stress responses is largely underexplored. We focused on OsTET5, which is differentially expressed under various abiotic stresses and localizes to both plasma membrane and endoplasmic reticulum. Using overexpression and underexpression transgenic lines we demonstrate that OsTET5 contributes to salinity and drought stress tolerance in rice. OsTET5 can interact with itself in yeast, suggesting homomer formation. Immunoblotting of native PAGE of microsomal fraction enriched from OsTET5‐Myc transgenic rice lines revealed multimeric complexes containing OsTET5, suggesting the potential formation of TEM complexes. Transcriptome analysis, coupled with quantitative PCR‐based validation, of OsTET5‐altered transgenic lines unveiled the differential expression patterns of several stress‐responsive genes, as well as those coding for transporters under salt stress. Notably, OsTET5 plays a crucial role in maintaining the ionic equilibrium during salinity stress, particularly by preserving an elevated potassium‐to‐sodium (K+/Na+) ratio. OsTET5 also regulates reactive oxygen species homeostasis, primarily by modulating the gene expression and activities of antioxidant pathway enzymes and proline accumulation. Our comprehensive investigation underscores the multifaceted role of OsTET5 in rice, accentuating its significance in developmental processes and abiotic stress tolerance. These findings open new avenues for potential strategies aimed at enhancing stress resilience and making valuable contributions to global food security.
Exploring possible causes of lower‐than‐expected suicide rates in Taiwan and South Korea during the 2020–2021 COVID‐19 pandemic: a time trend analysis by sex, age, and method
Authors: Lin Chien‐Yu , Choi Minjae , Lee Yo Han , Ki Myung , Hsu Chia‐Yueh , Chang Shu‐Sen 
Published: October 2024

AimsDecreases in suicide rates during the coronavirus disease 2019 (COVID‐19) pandemic were found in several countries, including Taiwan and South Korea. We investigated the pattern of the reduction in suicide by sex, age, method, and outbreak period in the two countries.MethodsSuicide data for Taiwan (2015–2021) and South Korea (2017–2021) stratified by sex, age, method, and month were extracted from national mortality data files in the two countries. Negative binomial regression was used to estimate suicide rate ratios and 95% confidence intervals across outbreak and inter‐outbreak periods during the pandemic, relative to that expected based on pre‐pandemic trends, and their associations with economic and outbreak control stringency indicators.ResultsThere were fewer‐than‐expected suicides in Taiwan (7%–16% fewer suicides over outbreaks and inter‐outbreaks) and South Korea (17% fewer suicides in outbreaks III and IV). Fewer‐than‐expected suicides were found primarily in the working‐age populations aged 25 to 64 years in Taiwan and those aged 45 to 64 years in South Korea. In both countries, fewer‐than‐expected suicides by charcoal burning during the pandemic were consistently found; the greatest reduction occurred when the outbreak control measures were most restricted. Increased time at residence was associated with decreased suicide rates in South Korea.ConclusionTaiwan and South Korea showed reduced suicide rates during the COVID‐19 pandemic in 2020–2021. Potential reasons for the decrease in suicides may include reduced access to suicide means during outbreaks in the two countries.
Fungal communities associated with postharvest strawberries in Western Australia
Authors: Momtaz Farhana , Hardy Giles , Bayliss Kirsty L. 
Published: October 2024

AbstractSpoilage of postharvest strawberries by fungal pathogens is a major global concern. This study employed culture‐dependent and culture‐independent methods to identify postharvest fungal communities of strawberries in Western Australia (WA). Ripe strawberries from eight varieties were sampled twice, early and late in the growing season, from 20 farms in two growing regions. At the first sampling time, traditional isolation and identification demonstrated a high abundance of Botrytis and Cladosporium in Region 1. Mucor was abundant at the second sampling time in Region 1 and both times in Region 2. Metabarcoding confirmed variety‐specific fungal communities in WA strawberries. Botrytis and Cladosporium were predominant in Region 1 and Region 2 samples with more than 50% read abundance except on variety Suncoast. No Mucor sequences were detected using metabarcoding, although they were isolated from all samples using culture‐based methods. Sisquoc, a hydroponic variety, had a significantly higher fungal diversity than the soil‐grown varieties. Varieties Suncoast and Albion exhibited the lowest fungal diversity compared to others. This study demonstrated the value of using culture‐dependent and culture‐independent methods to investigate the complexity of fungal microbiomes of postharvest strawberries.
Complete genome sequence of the avian paramyxovirus serotype 9 strain duck/Miyazaki/128/2021
Authors: Mekata Hirohisa , Yamamoto Mari , Matsui Yuto , Niazi Ahmad Massoud , Yamada Kentaro , Okabayashi Tamaki , Cha Se-Yeoun , Jang Hyung-Kwan 
Published: October 2024

ABSTRACT

Here, we report the complete genome sequence of the avian paramyxovirus serotype 9 strain duck/Miyazaki/128/2021, which was determined using the Illumina MiSeq platform. The position of the hemagglutinin-neuraminidase stop codon differed from that of the only other available completely sequenced prototype strain, duck/New York/22/1977.
Impact of community-based multidomain preventive health program on healthcare utilisation among elderly persons in Singapore: a propensity score matched and difference-in-differences study
Authors: Phng Francis , Yap Adrian Ujin , Teo Wesley , Chong Jesslyn Hwei Sing , Wu Christine Xia , Tan Eugene Eng Kee , Chin Chi Hsien , Chee Thong Gan , Lee Hee Hoon , Phan Phillip , Chua Ai Ping 
Published: October 2024

ObjectivesAgeing populations present substantial challenges for healthcare systems. Community-based health worker (CHW) interventions for promoting healthy ageing and preventing/managing non-communicable diseases have gained considerable attention in recent years. This study aimed to assess the impacts of a multidomain preventive health initiative delivered by CHWs, specifically the ‘My Health Map (MHM)’ programme, on participants’ health service utilisation using a propensity score matching methodology.Design, setting and participantsThe multidomain MHM programme, which was implemented in Bukit Batok township, encompassed screenings, vaccinations, chronic disease management, counselling and socio-environmental interventions. Individuals, aged ≥40 years old, who received care at Ng Teng Fong General Hospital and were enrolled in the MHM programme constituted the intervention group. Outcomes of the intervention group were compared with a 1:1 propensity-matched comparison group at enrolment and 1-year follow-up. The outcome measures were emergency department (ED) utilisation and hospital admissions. Statistical evaluations were performed using χ2/non-parametric tests and difference-in-difference (DiD) estimation with a bias-adjusted generalised estimating equation (α=0.05).ResultsA comparable comparison group was formed with no significant differences in baseline characteristics between groups. Data from a total of 299 MHM participants (mean age 70.7 (SD 9.6); 62.5% women) and 299 matched comparisons (mean age 72.1 (SD 16.6); 61.5% women) were appraised. DiD analysis indicated a significant reduction in ED attendance (−16.7%, p<0.001) and hospital admission (−18.4%, p<0.001) among intervention participants than the comparison participants.ConclusionsThe multidomain MHM programme proved effective in reducing ED attendances and hospital admissions in older adults. CHWs have the potential to serve as change agents in healthcare and should be systematically integrated into preventive health programmes.
Cancer cell-specific PD-L1 expression is a predictor of poor outcome in patients with locally advanced oral cavity squamous cell carcinoma
Authors: Wang Minyu , Qin Lei , Thia Kevin , Nguyen Thu , MacDonald Sean , Belobrov Simone , Kranz Sevastjan , Goode David , Trapani Joseph A , Wiesenfeld David , Neeson Paul Joseph 
Published: October 2024

BackgroundLocally advanced oral cavity squamous cell carcinoma (OCSCC) presents a significant clinical challenge despite being partially responsive to standard treatment modalities. This study investigates the prognostic implications of programmed death-ligand 1 (PD-L1) expression in these tumors, focusing on its association with treatment outcomes and the immune microenvironment.MethodsWe assessed tumor-infiltrating lymphocytes (TILs) in 132 patients with OCSCC to evaluate their impact on survival. Multiplex immunohistochemistry staining for CD3, CD68, CD11c, PD-L1, and P40 was used to explore correlations with clinical outcomes in patients with early-stage (n=22) and locally advanced (n=36) OCSCC. These initial findings were validated through differential gene expression analysis, gene set enrichment, and immune cell deconvolution in a The Cancer Genome Atlas cohort of 163 locally advanced OCSCC tumors. Additionally, single-cell RNA sequencing (scRNA-seq) on a smaller cohort (n=10) further characterized the PD-L1hior PD-L1locancer cells in these tumors.ResultsElevated PD-L1 expression was associated with poor outcomes in patients with locally advanced OCSCC undergoing standard adjuvant therapy, irrespective of “hot” or “cold” classification based on TILs assessment. PD-L1hitumors exhibited an active immune response phenotype, enriched with M1 macrophages, CD8+T cells and T regulatory cells in the tumor microenvironment. Notably, the negative impact of PD-L1 expression on outcomes was primarily attributed to its expression by cancer cells, rather than immune cells. Furthermore, scRNA-seq revealed that immune interactions were not essential for PD-L1 upregulation in cancer cells, instead, complex regulatory networks were involved. Additionally, PD-L1lolocally advanced tumors exhibited more complex pathway enrichment and diverse T-cell populations compared with those in the early-stage.ConclusionOur findings underscore the prognostic significance of PD-L1 expression in locally advanced OCSCC, and unveil the complex interplay between PD-L1 expression, immune responses, and molecular pathways in the tumor microenvironment. This study provides insights that may inform future therapeutic strategies, including the possibility of tailored immunotherapeutic approaches for patients with PD-L1hilocally advanced OCSCC.
What do family caregivers of patients with life-threatening diseases need from healthcare professionals? A qualitative study
Authors: Hoffstädt Hinke Elisabeth , Tam Marcella Cecilia , Stoppelenburg Arianne , Stoevelaar Rik , van Bodegom-Vos Leti , van der Steen Jenny Theodora , van der Linden Yvette Milene , Hartog Iris Dewi 
Published: October 2024

Objectives
To explore the common ground of what family caregivers need in their various roles (caregiver, care recipient and patient’s partner, child or friend) from healthcare professionals across healthcare settings and disease trajectories.

Design
Interviews were conducted with family caregivers of patients with life-threatening diseases who were treated at home, in hospitals, nursing homes or hospices between 2017 and 2022. Reflexive thematic analysis was performed.

Setting/participants
63 family caregivers of 65 patients were interviewed. Of the patients, 36 (55%) had COVID-19 and 29 (45%) had other life-threatening diseases (eg, advanced cancer, dementia). The majority of family caregivers were women (83%) and children of the patient (56%).

Results
Three themes were developed regarding family caregivers’ core needs across their different roles: (1) ‘feeling seen and valued’, (2) ‘experiencing trust in the provided care’ and (3) ‘experiencing guidance and security’. Actions of healthcare professionals that meet those needs relate to their contact and relationship with family caregivers, information provision, practical and emotional support, the care for the patient and facilitating the connection between family caregivers and patients.

Discussion
Healthcare professionals should be trained in meeting family caregivers’ core needs, in which their (collaborative) relationship with them plays an important role. Efforts to meet the core needs should be incorporated into healthcare organisations’ workflows, and future research should investigate related barriers and facilitators.
Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage
Authors: Zhu Huimin , Wang Ningning , Chang Yingying , Zhang Ying , Jiang Shihe , Ren Xiaoping , Yuan Meng , Chang Haoxiao , Jin Wei-Na 
Published: October 2024

IntroductionIntracerebral haemorrhage (ICH) elicits a robust inflammatory response, which significantly contributes to secondary brain damage. Extracellular vesicles (EVs) play a pivotal role in intercellular communication by transporting immune-regulatory proteins. However, the precise contribution of these EV-carried proteins to neuroinflammation following ICH remains elusive. Here, we identified proteins dysregulated in EVs and further studied the EVs-enriched Serum amyloid A 1 (SAA1) to understand its role in neuroinflammation and ICH injury.MethodsWe used mass spectrometry to analyse the EV protein cargo isolated from plasma samples of 30 ICH patients and 30 healthy controls. To validate the function of the dysregulated protein SAA1, an ICH mouse model was conducted to assess the effects of SAA1 neutralisation on brain oedema, neurological function and infiltration of peripheral leucocytes.Results49 upregulated proteins and 12 downregulated proteins were observed in EVs from ICH patients compared with controls. Notably, SAA1 demonstrated a significant increase in EVs associated with ICH. We observed that exogenous SAA1 stimulation led to an augmentation in the population of microglia and astrocytes, exacerbating neuroinflammation. Neutralising SAA1 with an anti-SAA1 monoclonal antibody (mAb) diminished the prevalence of proinflammatory microglia and the infiltration of peripheral leucocytes, which ameliorates brain oedema and neurological function in ICH mice.ConclusionOur findings provide compelling evidence implicating EVs and their cargo proteins in ICH pathogenesis. SAA1 emerges as a potential therapeutic target for mitigating neuroinjury and neuroinflammation following ICH.