bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring and assessment of polycyclic aromatic hydrocarbons in pizza samples (meat and chicken) using modified MSPE extraction and GC/MS method
Authors: Shariatifar Nabi , Arabameri Majid , Moazzen Mojtaba , Shavli Parisa , Hadian Zahra 
Published: October 2024

Abstract

The objective of present research was to assess the PAH (polycyclic aromatic hydrocarbon) concentrations in Pizza (chicken and beef) cooked, by technique of MSPE-GC/MS (magnetic solid phase extraction-gas chromatography mas spectrophotometry). The outcomes revealed the mean ± SD of PAH4, ∑PAHs and BaP in all pizza samples was 2.97 ± 1.82, 12.75 ± 2.1 and 0.18 ± 0.02µg/kg, respectively. Based on the present results, in meat pizza samples, the mean ± SD (min-max) of PAH4, total PAHs and BaP was 4.20 ± 0.9 14.90 ± 1.59 and 0.31 ± 0.02 µg/kg, respectively, and in chicken pizza samples was 1.5 ± 0.8 10.18 ± 1.96 and 0.03 ± 0.01, respectively. A Heat map was used to understand the individual similarities and distinctions between PAHs detected in each sample with color intensity. The Based on the Monte Carlo consequences achieved, the EDI (estimated daily intake) of PAH measured was ranked as B(k)F > Fl > B(a)P > B(b)F > B(a)A > Ph > CHR > I(1,2,3-cd)P > D(a,h)A. The ILCR (incremental lifetime cancer risk) for the children and adults was 2.28E-8 and 6.07E-9, respectively. The research’s outcomes indicate the contents of PAH in pizza (chicken and beef) do not pose a safety concern for consumers in Iran (ILCR < 10− 6).
Comprehensive analysis of the cardiac whole-transcriptome expression profiling involved in rehabilitation exercise improving myocardial remodeling after acute myocardial infarction
Authors: Chen Xueying , Chen Mingrui , Li Zhongxin , Yan Yugang , Lin Nan , Ni Liangchun , Zhang Yang , Kang Nan , Gao Haizhu , Guo Cuimei , Bian Xinxin , Zhang Shaohui , Zhang Qingyun , Gan Lijun 
Published: October 2024

Abstract

Rehabilitation exercise (RE) has been shown to mitigate cardiac remodeling and enhance cardiac function in patients with acute myocardial infarction (AMI). However, the precise molecular mechanisms underlying these effects remain incompletely elucidated. In this study, we established a mice model of acute myocardial infarction (AMI) and implemented an 8-week rehabilitation exercise intervention. Whole-transcription sequencing was conducted to examine the expression patterns of mRNA and non-coding RNAs (ncRNAs) in the myocardium of mice with AMI, with and without rehabilitation exercise (RE). GO, KEGG pathway enrichment and protein-protein interaction (PPI) network analyses were performed. And the mRANs within the ceRNA network were validated by RT-PCR. Our findings demonstrate that RE effectively enhances cardiac function, attenuates fibrosis and promotes angiogenesis in the myocardial tissue following MI. Furthermore, bio informatics tools and databases were utilized to investigate potential functions and associations of non-coding RNAs. Our data revealed that: a total of 100 long ncRNAs (lncRNAs), 14 microRNAs (miRNAs), 131 circular RNAs (circRNAs), and 1028 messenger RNAs (mRNAs) were significant. The most prominent pathways involved in RE-mediated improvement of cardiac remodeling after AMI are the PI3K-Akt signaling pathway, cytokine − cytokine receptor interaction, chemokine signaling pathway and MAPK signaling pathway. In addition, lncRNA‒miRNA-mRNA and circRNA-miRNA‒mRNA networks of RE-mediated improvement of cardiac remodeling after AMI were constructed. The present study elucidates the physiological roles of mRNA and ncRNAs in facilitating cardiac remodeling post-AMI. Our findings establish a theoretical framework for investigating the mechanisms underlying cardiac remodeling after AMI, while also providing valuable insights for exploring potential therapeutic interventions for AMI.
Development and validation of a prediction model for intrapartum fever related to chorioamnionitis in parturients undergoing epidural analgesia
Authors: Ling Liang , Liu Bo , Li Chunping , Zhang Dan , Jia Fei , Tang Yong , Chen Benzhen , Wang Mengqiao , Zhang Jian 
Published: October 2024

Abstract

Intrapartum fever is a common complication in parturients undergoing epidural analgesia(EA), significantly increasing the incidence of maternal and infant complications. This study aims to develop and validate a prediction model for intrapartum fever related to chorioamnionitis(IFTC) in parturients undergoing epidural analgesia. A total of 596 parturients with fever (axillary temperature ≥ 38℃) who received EA from January 2020 to December 2023 were included and randomly assigned to the training set (N = 417) and the validation set (N = 179) according to the ratio of 7:3. The independent risk factors were screened by univariate and multivariate logistic regression analysis to develop a nomogram model. Decision curve analysis (DCA) was used to evaluate the clinical effectiveness and discrimination of the model.;calibration curve was used to assess the accuracy of the model. Maximum temperature, meconium-stained amniotic fluid, C-reactive protein (CRP), gestational age and BMI were independent risk factors for predicting IFTC,and the area under receiver operating characteristic curve(AUC) of the training set and the validation set were 0.744 (0.691–0.796) and 0.793 (0.714–0.872), respectively. The calibration curve showed good consistency between predicted and actual results. DCA curve showed that the model had clinical value throughout a broad threshold probability range. The nomogram prediction model based on CRP, meconium-stained amniotic fluid, maximum temperature, gestational age and BMI has good predictive performance for the risk of IFTC in EA parturients.
The advisability of science fair project topic origins: a case study of learners from disadvantaged backgrounds
Authors: Stott Angela Elisabeth 
Published: October 2024

Abstract

For learners from disadvantaged backgrounds with limited science fair-related skills, knowledge, and community support, investigating the advisability of various ways of arriving at a science fair project topic is needed. This mixed-methods case study uses questionnaire data and science fair awards regarding the engagement of 23 grade 9-10 learners in a year-long university-led intervention. Data analysis was conducted for four post-hoc determined topic-origin groups of these learners’ projects: internet, self, adult, and adult after the learner had displayed competence. Descriptive statistics and thematic analysis were used to determine whether each topic-origin group met the advisability criteria regarding learners’ perceptions of value and facilitators’ perceptions of feasibility. Each learner who was provided with a topic by an adult without first having displayed competence through designing a project of their choosing required unfeasibly high levels of facilitator input to remain in the programme. Generally, the greatest success was observed for the learners to whom an adult gave a topic after displaying competence. These findings and how the intervention was conducted are explained in terms of the four paradigms of learning and instructional design. A framework (Instruct-Expose-Explore-Formalise) for deriving science fair topics is suggested to enhance the efficiency and effectiveness of similar interventions.
Abnormalities in Brain Complexity in Children with Autism Spectrum Disorder: A Sleeping State Functional MRI Study.
Authors: Fu Shishun , Wang Xiang , Chen Ziwei , Huang Zengfa , Feng Yin , Xie Yuanliang , Li Xiang , Yang Chunlan , Xu Shoujun 
Published: October 2024

Abstract

Background and Objective: The theory of complexity loss in mentally disordered brain is widely acknowledged. However, the findings of autism research do not seem to align well with this theory. We aim to investigate the brain complexity in children with ASD (Autism Spectrum Disorders) compared with the TD (Typical Developed) children in sleeping state.
Method: 42 ASD children and 42 TD children were imaged using sleep-state functional magnetic resonance imaging (ss-fMRI), and brain complexity was analyzed by employing sample entropy (SampEn) and transfer entropy (TE). For the ASD group, we also investigated the relationship of symptom severity with SampEn and with TE.
Results: In compared with TD group, ASD group showed significant elevated SampEn in the right inferior frontal gyrus. However, in the group of TD, 13 pairs of brain regions exhibit higher TE compared to the ASD group. In the ASD group, the TE of 5 pairs of brain regions is higher than in the TD group. A positive correlation was found between cerebellum-amygdala TE in ASD group and ABC total scores.
Conclusion: This sleeping-state fMRI study provide evidence that ASD children exhibited aberrant brain complexity in compare with the TD children. The complexity of the autistic brain is composed of aberrant randomness in brain activity and anomalous information transmission between brain regions. We propose that the abnormally diminished quantity of information transmission may potentially underlie the decreased complexity of the autistic brain.
Toward precision medicine: Exploring proteomic signatures in sepsis and non-infectious systemic inflammatory response syndrome
Authors: Ruiz-Sanmartín Adolfo , Ribas Vicent , Suñol David , Chiscano-Camón Luis , Martín Laura , Bajaña Iván , Bastida Juliana , Larrosa Nieves , González Juan José , Carrasco María Dolores , Canela Núria , Ferrer Ricard , Ruiz-Rodríguez Juan Carlos 
Published: October 2024

Abstract

Background
The search for new biomarkers that allow an early diagnosis in sepsis has become a necessity in medicine. The objective of this study is to identify potential protein biomarkers of differential expression between sepsis and non-infectious systemic inflammatory response syndrome (NISIRS).
Methods
Prospective observational study of a cohort of septic patients activated by the Sepsis Code and patients admitted with NISIRS, during the period 2016–2017. A mass spectrometry-based approach was used to analyze the plasma proteins in the enrolled subjects. Subsequently, using recursive feature elimination (RFE) classification and cross-validation with a vector classifier, an association of these proteins in patients with sepsis compared to patients with NISIRS. The protein-protein interaction network was analyzed with String software.
Results
277 patients were included (141 with sepsis and 136 with NISIRS). After performing RFE, 30 proteins (SERPINA4, ITIH1, ITIH3, SERPINA3, F12, FN1, SERPINA6, APOE, GSN, C3, SERPINF1, C5, LBP, CD14, FCN3, C6, C1RL, PRDX2, APOB, PPBP, SAA1, VWF, LRG1, AFM, BTD, ORM1, RBP4, LUM, COL1A1, CA1) demonstrated an association with sepsis compared to patients with NISIRS with an accuracy of 0.49 ± 0.035, precision of 0.967 ± 0.037, specificity of 0.910 ± 0.103, sensitivity of 0.964 ± 0.035 and an area under the curve (AUC) of 0.937. Of these PPBP, V1RL, C5, vWF and SERPINA4 have a greater association with Sepsis compared to NISIRS.
Conclusion
There are proteomic patterns associated with sepsis compared to NISIRS with different strength of association. Advances in understanding these protein changes may allow for the identification of new biomarkers or therapeutic targets in the future.
Fibroblasts derived C3 promotes the progression of experimental periodontitis through macrophage M1 polarization and osteoclast differentiation
Authors: Sun Hongchen , ren feilong , Zheng Shize , Luo Huanyu , Yu Xiaoyi , Li Xianjing , Song Shaoyi , Bu Wenhuan 
Published: October 2024

Abstract

Complement C3 plays a critical role in periodontitis. However, its source, role and underlying mechanisms remain unclear. In our study, by analyzing single-cell sequencing data from mouse model of periodontitis, we identified that C3 is primarily derived from periodontal fibroblasts. Subsequently, we demonstrated that C3a has a detrimental effect in ligature-induced periodontitis. C3ar-/- mice exhibited significantly less destruction of periodontal support tissues compared to wild-type mice, characterized by mild gingival tissue damage and reduced alveolar bone loss. This reduction was associated with decreased production of pro-inflammatory mediators and reduced osteoclast infiltration in the periodontal tissues. Mechanistic studies suggested that C3a could promote macrophage polarization and osteoclast differentiation. Finally, by analyzing single-cell sequencing data from the periodontal tissues of patients with periodontitis, we found that the results observed in mice were consistent with human data. Therefore, our findings clearly demonstrate the destructive role of fibroblast-derived C3 in ligature-induced periodontitis, driven by macrophage M1 polarization and osteoclast differentiation. These data strongly support the feasibility of C3a-targeted interventions for the treatment of human periodontitis.
Refractory testicular germ cell tumors are highly sensitive to the targeting of polycomb pathway demethylases KDM6A and KDM6B
Authors: Shokry Doha , Khan Mehwish W , Powell Christine , Johnson Samantha , Rennels Brayden C. , Boyd Raya I. , Sun Zhengyang , Fazal Zeeshan , Freemantle Sarah J. , Parker Maryanna H. , Vieson Miranda D. , Samuelson Jonathan P. , Spinella Michael J. , Singh Ratnakar 
Published: October 2024

Abstract

Testicular germ cell tumors (TGCTs) can be treated with cisplatin-based therapy. However, a clinically significant number of cisplatin-resistant patients die from progressive disease as no effective alternatives exist. Curative cisplatin therapy results in acute and life-long toxicities in the young TGCT patient population providing a rationale to decrease cisplatin exposure. In contrast to genetic alterations, recent evidence suggests that epigenetics is a major driving factor for TGCT formation, progression, and response to chemotherapy. Hence, targeting epigenetic pathways with “epidrugs” is one potential relatively unexplored strategy to advance TGCT treatment beyond cisplatin. In this report, we demonstrate for the first time that targeting polycomb demethylases KDM6A and KDM6B with epidrug GSK-J4 can treat both cisplatin-sensitive and -resistant TGCTs. While GSK-J4 had minimal effects alone on TGCT tumor growth in vivo, it dramatically sensitized cisplatin-sensitive and -resistant TGCTs to cisplatin. We validated KDM6A/KDM6B as the target of GSK-J4 since KDM6A/KDM6B genetic depletion had a similar effect to GSK-J4 on cisplatin-mediated anti-tumor activity and transcriptome alterations. Pharmacologic and genetic targeting of KDM6A/KDM6B potentiated or primed the p53-dominant transcriptional response to cisplatin, with also evidence for basal activation of p53. Further, several chromatin modifier genes, including BRD4, lysine demethylases, chromodomain helicase DNA binding proteins, and lysine methyltransferases, were repressed with cisplatin only in KDM6A/KDM6B-targeted cells, implying that KDM6A/KDM6B inhibition sets the stage for extensive chromatin remodeling of TGCT cells upon cisplatin treatment. Our findings demonstrate that targeting polycomb demethylases is a new potent pharmacologic strategy for treating cisplatin resistant TGCTs that warrants clinical development.
Effects of rapid enteral feed advancement with early human milk fortification in
preterm infants &gt; 30 weeks gestation in a resource limited setting- a randomized
controlled trial
Authors: Haribalakrishna Anitha , Bhakar Ganesh , Balasubramanian Haribalakrishna , Vijayanathan Muthu , Sriniva Lakshmi 
Published: October 2024

Abstract

Objective: To study the effects of rapid enteral feed advancement with early feed fortification in stable very low birth weight (VLBW) infants >30 weeks gestation.
Study design: Preterm infants (N=92) were randomized to a) rapid feed advancement-early fortification - REF group (enteral feed advanced at 25-30ml/kg/day, fortification at 50ml/kg/day) or b) slow feed advancement-late fortification-SLF group (feed advanced at 15-20ml/kg/day, fortification at 100ml/kg/day).
Results: The primary outcome-time to regain birth weight was significantly lower in REF group (9 days vs 13 days, P=0.02). REF group reached full enteral feeds earlier (6 days vs 9 days, P=0.001), had lower rates of sepsis (13% vs 38%, P=0.007) and shorter hospital stay (10 days vs 15 days, P=0.01). At one year, the median Z-scores for weight [-1.5 vs -2.2, P<0.001] and head circumference [1.1 vs 0, P<0.001) were significantly higher in the REF group.
Conclusions: In VLBW infants >30 weeks, rapid feed advancement with early fortification resulted in early postnatal regain of birth weight with positive effects on growth at one year.
The Immune System’s Role in Glymphatic System Clearance and Alzheimer’s Disease Risk: A Csf Proteome-wide Study
Authors: Cullell Natalia , Caruana Giovanni , Elias Andrea , Delgado-Sanchez Ariane , Artero Cristina , Buongiorno Maria Teresa , Almería Marta , Ray Nicola J , Correa Sonia A.L. , Krupinski Jerzy 
Published: October 2024

Abstract

Background: The emerging evidence of the role of the glymphatic system (GS) in Alzheimer’s disease (AD) provides new opportunities for intervention from the earliest stages of the disease. The aim of the study is to evaluate the efficacy of GS in AD to identify new disease biomarkers.
Methods: We performed a two-stage proteomic study to evaluate the GS health using intravenous gadolinium-based contrast agent (GBCA) with serial T1 3T magnetic resonance imaging (MRI) in individuals with amnestic mild cognitive impairment (a-MCI). In Stage 1 (evaluated in the Cohort 1 of a-MCI participants (n=11)), we correlated the levels of 7K CSF proteins (estimated by SOMAscan) with GS health in 78 Freesurfer-segmented brain regions of interest (ROIs).
Results: A total of 173 different proteins were significantly associated with GS health (p-value < 10-3). The stronger correlations were identified for GRAAK, NSUN6, SHPS1 and TIM-4. A pathway enrichment analysis revealed that the proteins associated with GS health were mainly implicated in neurodegenerative processes, immunity and inflammation. In Stage 2, we validated these proteomic results in a new cohort of a-MCI participants (with and without evidence of AD pathology in CSF (aMCI(-) and aMCI(+); n = 22 and 7, respectively) and healthy controls (n = 10). Proteomic prediction models were generated in each ROI. These were compared with demographic-only models for identifying participants with aMCI(-) and aMCI(+) vs controls. This analysis was repeated to determine if the models could identify those with aMCI(+) from both aMCI(-) and controls. The proteomic models were found to outperform the demographic-only models.
Conclusions: Our study identifies proteins linked with GS health and involved the immune system in a-MCI participants.
Comprehensive upper-limb human-machine interaction strategies for post-stroke upper-limb rehabilitation
Authors: Xia Kang , Chang Xue-Dong , Liu Chong-Shuai , Sun Han , Wang Yi-Min , Wang Xin-Wei 
Published: October 2024

Abstract

Background
Stroke and its related complications, place significant burdens on human society in the 21st century, and lead to substantial demands for rehabilitation. To fulfill rehabilitation needs, human-machine interaction (HMI) technology strives continuously. Traditional passive HMI strategy requires device to be dynamically well-fitting, and executes command precisely. Advanced active HMI strategy also demands the device to react fast and accurately based on user’s intention. However, current study usually focuses on limited aspect of HMI, a complete HMI study which addresses the complexity of stroke related complications and provide the possibility for personalized post-stroke upper-limb rehabilitation is lacking.
Method
An Up-limb Rehabilitation Device and Utility System (UarDus) is proposed along with 3 HMI strategies namely robot-in-charge, therapist-in-charge and patient-in-charge. Based on physiological structure of human upper-limb and scapulohumeral rhythm (SHR) of shoulder, a base exoskeleton with 14 degrees of freedoms (DoFs) is designed as foundation of the 3 strategies. Passive robot-in-charge and therapist-in-charge strategies provides fully-assisted rehabilitation options. The active patient-in-charge strategy incorporates data acquisition matrices and a new deep learning model, which is developed based on CNN and Transformer structure, aims to provide partially-assisted rehabilitation.
Results
Kinematically, the work space of the base exoskeleton is presented first. Utilizing motion capture technology, the GH center of both human and exoskeleton is compared the well-matched curves suggesting comfortable dynamic wear experience. For robot-in-charge and therapist-in-charge strategy, the desired and measured angle-time curve present good correlation, with low phase difference, which serve the purpose of real-time control. Featuring the patient-in-charge strategy, Kernel Density Estimation (KDE) result suggesting reasonable sensor-machine-human synergy. Applying K-fold (K = 10) cross-validation method, the classification accuracy of the proposed model achieves an average of 99.6% for the designated 15 actions. The proposed model is also evaluated on public datasets, demonstrating outstanding response time and accuracy comparing with state-of-art models.
Conclusions
A validated exoskeleton hardware system named UarDus is constructed, along with the 3 HMI strategies proposed. This work provides possibility for people with stroke to engage in complex personalized rehabilitation training with dynamic comfortable wear experience.
Levodopa responsive parkinsonism in Spinocerebellar ataxia 27B
Authors: El-Masri Shaddy , Wijaithum Piyanit , Chugh Pooja , Te Joshua , Triplett James 
Published: October 2024

Abstract

Background:
Spinocerebellar ataxia 27B (SCA27B) is a recently characterised cause of individuals presenting with late onset cerebellar ataxia. Here, we present a case of late onset cerebellar ataxia with associated Parkinsonism that is responsive to levodopa.
Case presentation:
A 65 year-old female presents with a progressive deterioration in her gait secondary to truncal ataxia since the age of 62 years. Throughout the course of her illness, she developed asymmetrical parkinsonism, bowel and bladder incontinence, and had a background of parasomnias preceding her presentation and also a family history of late onset parkinsonism and early onset cognitive impairment. Brain magnetic resonance imaging revealed atrophy of the midbrain, PONS, and middle cerebellar peduncles. Genetic testing confirmed a pathogenic triplet repeat within the FGF14 gene consistent with a diagnosis of SCA27B. Levodopa was commenced for her parkinsonism, with improvement in the speed and amplitude of her movements, and restoration of partial independence.
Conclusion
SCA27B should be considered in individuals presenting with late onset ataxia, and may mimic alternate neurodegenerative conditions. This case report demonstrates that associated parkinsonism may be responsive to levodopa.