bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic control of influenza virus: role of H3K79 methylation in interferon-induced antiviral response
Authors: Marcos-Villar Laura , Díaz-Colunga Juan , Sandoval Juan , Zamarreño Noelia , Landeras-Bueno Sara , Esteller Manel , Falcón Ana , Nieto Amelia 
Published: January 2018

AbstractInfluenza virus stablishes a network of virus-host functional interactions, which depends on chromatin dynamic and therefore on epigenetic modifications. Using an unbiased search, we analyzed the epigenetic changes at DNA methylation and post-translational histone modification levels induced by the infection. DNA methylation was unaltered, while we found a general decrease on histone acetylation, which correlates with transcriptional inactivation and may cooperate with the impairment of cellular transcription that causes influenza virus infection. A particular increase in H3K79 methylation was observed and the use of an inhibitor of the specific H3K79 methylase, Dot1L enzyme, or its silencing, increased influenza virus replication. The antiviral response was reduced in conditions of Dot1L downregulation, since decreased nuclear translocation of NF-kB complex, and IFN-β, Mx1 and ISG56 expression was detected. The data suggested a control of antiviral signaling by methylation of H3K79 and consequently, influenza virus replication was unaffected in IFN pathway-compromised, Dot1L-inhibited cells. H3K79 methylation also controlled replication of another potent interferon-inducing virus such as vesicular stomatitis virus, but did not modify amplification of respiratory syncytial virus that poorly induces interferon signaling. Epigenetic methylation of H3K79 might have an important role in controlling interferon-induced signaling against viral pathogens.
Early lexical processing of Chinese words indexed by Visual Mismatch Negativity effects
Authors: Wei Dawei , Gillon Dowens Margaret , Guo Taomei 
Published: January 2018

AbstractAlthough Mismatch Negativity (MMN) effects indicating early, automatic lexical processing have been reported in the auditory language modality, so far these have not been reliably obtained in MMN studies of visual word recognition. The present study explores this discrepancy by investigating whether visual MMN (vMMN) effects can be obtained in written Chinese single-character word recognition. While participants were engaged in a non-linguistic distraction task, we measured Event-Related Potentials (ERPs) time-locked to perifoveally-presented real and pseudo- characters matched in overall visual-orthographic attributes. VMMN was defined as significant difference between the ERPs to characters presented as deviants or as standards in a context of non-characters. For the native Chinese readers, af ter sub-lexical structural detection from 120–160 ms, only real characters elicited vMMN at the interval of 170–210 ms, suggesting that lexical information in Chinese words is processed early and automatically. In a later window of 340–380 ms, both real and pseudo- characters yielded vMMNs. In a control group of non-Chinese participants, no evidence of vMMN was found for either real or pseudo-characters. Taken together, these results suggest that long-term memory representations for real characters may enable their early processing even in unattended conditions.
Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma
Authors: Connolly Nina P. , Shetty Amol C. , Stokum Jesse A. , Hoeschele Ina , Siegel Marni B. , Miller C. Ryan , Kim Anthony J. , Ho Cheng-Ying , Davila Eduardo , Simard J. Marc , Devine Scott E. , Rossmeisl John H. , Holland Eric C. , Winkles Jeffrey A. , Woodworth Graeme F. 
Published: January 2018

AbstractGlioma is a unique neoplastic disease that develops exclusively in the central nervous system (CNS) and rarely metastasizes to other tissues. This feature strongly implicates the tumor-host CNS microenvironment in gliomagenesis and tumor progression. We investigated the differences and similarities in glioma biology as conveyed by transcriptomic patterns across four mammalian hosts: rats, mice, dogs, and humans. Given the inherent intra-tumoral molecular heterogeneity of human glioma, we focused this study on tumors with upregulation of the platelet-derived growth factor signaling axis, a common and early alteration in human gliomagenesis. The results reveal core neoplastic alterations in mammalian glioma, as well as unique contributions of the tumor host to neoplastic processes. Notable differences were observed in gene expression patterns as well as related biological pathways and cell populations known to mediate key elements of glioma biology, including angiogenesis, immune evasion, and brain invasion. These data provide new insights regarding mammalian models of human glioma, and how these insights and models relate to our current understanding of the human disease.
Bi-exponential 3D-T1ρ mapping of whole brain at 3 T
Authors: Menon Rajiv G. , Sharafi Azadeh , Windschuh Johannes , Regatte Ravinder R. 
Published: January 2018

AbstractDetection of multiple relaxation pools using MRI is useful in a number of neuro-pathologies including multiple sclerosis (MS), Alzheimer’s, and stroke. In this study we evaluate the feasibility of using T1ρ imaging for the detection of bi-exponential decays in the human brain. A prospective T1ρ imaging study was performed on model relaxation phantoms (eggs) and 7 healthy volunteers. The data was fitted using a single pool and a 2-pool model to estimate mono- and bi-exponential T1ρ maps, respectively. Bi-exponential decays were identified in the gray matter (GM) and white matter (WM) of the brain with 40.5% of GM, and 65.1% of WM pixels showing two T1ρ relaxation pools (significance level P < 0.05). Detection of T1ρ based bi-exponential decays in the brain provides complimentary information to T2 based contrast regarding the in vivo micro-environment in the brain.
Early impoverished environment delays the maturation of cerebral cortex
Authors: Narducci Roberta , Baroncelli Laura , Sansevero Gabriele , Begenisic Tatjana , Prontera Concetta , Sale Alessandro , Cenni Maria Cristina , Berardi Nicoletta , Maffei Lamberto 
Published: January 2018

AbstractThe influence of exposure to impoverished environments on brain development is unexplored since most studies investigated how environmental impoverishment affects adult brain. To shed light on the impact of early impoverishment on developmental trajectories of the nervous system, we developed a protocol of environmental impoverishment in which dams and pups lived from birth in a condition of reduced sensory-motor stimulation. Focusing on visual system, we measured two indexes of functional development, that is visual acuity, assessed by using Visual Evoked Potentials (VEPs), and VEP latency. In addition, we assessed in the visual cortex levels of Insulin-Like Growth Factor 1 (IGF-1) and myelin maturation, together with the expression of the GABA biosynthetic enzyme GAD67. We found that early impoverishment strongly delays visual acuity and VEP latency development. These functional changes were accompanied by a significant reduction of IGF-1 protein and GAD67 expression, as well as by delayed myelination of nerve fibers, in the visual cortex of impoverished pups. Thus, exposure to impoverished living conditions causes a significant alteration of developmental trajectories leading to a prominent delay of brain maturation. These results underscore the significance of adequate levels of environmental stimulation for the maturation of central nervous system.
SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice
Authors: He Qi , Diao Yan , Zhao Tingting , Hou Baoyu , Ngokana Linel Darrel , Liang Huan , Nie Junhui , Tan Peizhu , Huang Hui , Li Yanze , Qi Lin , Zhao Yuanyuan , Liu Ying , Gao Xu , Zhou Lingyun 
Published: January 2018

AbstractCell death inducing DNA fragmentation factor-alpha-like A (Cidea) is a member of cell death-inducing DFF45-like effector (CIDE) protein. The initial function of CIDE is the promotion of cell death and DNA fragmentation in mammalian cells. Cidea was recently reported to play critical roles in the development of hepatic steatosis. The purpose of present study is to determine the effect of chronic alcohol intake on Cidea expression in the livers of mice with alcoholic fatty liver disease. Cidea expression was significantly increased in the liver of alcohol-induced fatty liver mice. While, knockdown of Cidea caused lipid droplets numbers reduction. Next, we detected the activity of ALDH2 reduction and the concentration of serum acetaldehyde accumulation in our alcohol-induced fatty liver mice. Cidea expression was elevated in AML12 cells exposed to 100uM acetaldehyde. Interestingly, Dual-luciferase reporter gene assay showed that 100 uM acetaldehyde led to the activation of Cidea reporter gene plasmid which containing SRE element. What’s more, the knockdown of SREBP1c suppressed acetaldehyde-induced Cidea expression. Overall, our findings suggest that Cidea is highly associated with alcoholic fatty liver disease and Cidea expression is specifically induced by acetaldehyde, and this up-regulation is most likely mediated by SREBP1c.
Handheld magnetic probe with permanent magnet and Hall sensor for identifying sentinel lymph nodes in breast cancer patients
Authors: Sekino Masaki , Kuwahata Akihiro , Ookubo Tetsu , Shiozawa Mikio , Ohashi Kaichi , Kaneko Miki , Saito Itsuro , Inoue Yusuke , Ohsaki Hiroyuki , Takei Hiroyuki , Kusakabe Moriaki 
Published: January 2018

AbstractThe newly developed radioisotope-free technique based on magnetic nanoparticle detection using a magnetic probe is a promising method for sentinel lymph node biopsy. In this study, a novel handheld magnetic probe with a permanent magnet and magnetic sensor is developed to detect the sentinel lymph nodes in breast cancer patients. An outstanding feature of the probe is the precise positioning of the sensor at the magnetic null point of the magnet, leading to highly sensitive measurements unaffected by the strong ambient magnetic fields of the magnet. Numerical and experimental results show that the longitudinal detection length is approximately 10 mm, for 140 μg of iron. Clinical tests were performed, for the first time, using magnetic and blue dye tracers—without radioisotopes—in breast cancer patients to demonstrate the performance of the probe. The nodes were identified through transcutaneous and ex-vivo measurements, and the iron accumulation in the nodes was quantitatively revealed. These results show that the handheld magnetic probe is useful in sentinel lymph node biopsy and that magnetic techniques are widely being accepted as future standard methods in medical institutions lacking nuclear medicine facilities.
Equal status in Ultimatum Games promotes rational sharing
Authors: Han Xiao , Cao Shinan , Bao Jian-Zhang , Wang Wen-Xu , Zhang Boyu , Gao Zi-You , Sánchez Angel 
Published: January 2018

AbstractExperiments on the Ultimatum Game (UG) repeatedly show that people’s behaviour is far from rational. In UG experiments, a subject proposes how to divide a pot and the other can accept or reject the proposal, in which case both lose everything. While rational people would offer and accept the minimum possible amount, in experiments low offers are often rejected and offers are typically larger than the minimum, and even fair. Several theoretical works have proposed that these results may arise evolutionarily when subjects act in both roles and there is a fixed interaction structure in the population specifying who plays with whom. We report the first experiments on structured UG with subjects playing simultaneously both roles. We observe that acceptance levels of responders approach rationality and proposers accommodate their offers to their environment. More precisely, subjects keep low acceptance levels all the time, but as proposers they follow a best-response-like approach to choose their offers. We thus find that status equality promotes rational sharing while the influence of structure leads to fairer offers compared to well-mixed populations. Our results are far from what is observed in single-role UG experiments and largely different from available predictions based on evolutionary game theory.
Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells
Authors: Deleye Lieselot , Vander Plaetsen Ann-Sophie , Weymaere Jana , Deforce Dieter , Van Nieuwerburgh Filip 
Published: January 2018

AbstractTo allow multiple genetic analyses on a single cell, whole genome amplification (WGA) is required. Unfortunately, studies comparing different WGA methods for downstream human identification Short Tandem Repeat (STR) analysis remain absent. Therefore, the aim of this work was to assess the performance of four commercially available WGA kits for downstream human identification STR profiling on a B-lymphoblastoid cell line. The performance was assessed using an input of one or three micromanipulated cells. REPLI-g showed a very low dropout rate, as it was the only WGA method in this study that could provide a complete STR profile in some of its samples. Although Ampli1, DOPlify and PicoPLEX did not detect all selected STR markers, they seem suitable for genetic identification in single-cell applications.
Phosphorylation of the Transient Receptor Potential Ankyrin 1 by Cyclin-dependent Kinase 5 affects Chemo-nociception
Authors: Hall Bradford E. , Prochazkova Michaela , Sapio Matthew R. , Minetos Paul , Kurochkina Natalya , Binukumar B. K. , Amin Niranjana D. , Terse Anita , Joseph John , Raithel Stephen J. , Mannes Andrew J. , Pant Harish C. , Chung Man-Kyo , Iadarola Michael J. , Kulkarni Ashok B. 
Published: January 2018

AbstractCyclin-dependent kinase 5 (Cdk5) is a key neuronal kinase that is upregulated during inflammation, and can subsequently modulate sensitivity to nociceptive stimuli. We conducted an in silico screen for Cdk5 phosphorylation sites within proteins whose expression was enriched in nociceptors and identified the chemo-responsive ion channel Transient Receptor Potential Ankyrin 1 (TRPA1) as a possible Cdk5 substrate. Immunoprecipitated full length TRPA1 was shown to be phosphorylated by Cdk5 and this interaction was blocked by TFP5, an inhibitor that prevents activation of Cdk5. In vitro peptide-based kinase assay revealed that four of six TRPA1 Cdk5 consensus sites acted as substrates for Cdk5, and modeling of the ankyrin repeats disclosed that phosphorylation would occur at characteristic pockets within the (T/S)PLH motifs. Calcium imaging of trigeminal ganglion neurons from genetically engineered mice overexpressing or lacking the Cdk5 activator p35 displayed increased or decreased responsiveness, respectively, to stimulation with the TRPA1 agonist allylisothiocyanate (AITC). AITC-induced chemo-nociceptive behavior was also heightened in vivo in mice overexpressing p35 while being reduced in p35 knockout mice. Our findings demonstrate that TRPA1 is a substrate of Cdk5 and that Cdk5 activity is also able to modulate TRPA1 agonist-induced calcium influx and chemo-nociceptive behavioral responses.
Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels
Authors: Kuno Takuya , Hirayama-Kurogi Mio , Ito Shingo , Ohtsuki Sumio 
Published: January 2018

AbstractAntibiotic-caused changes in intestinal flora (dysbiosis) can have various effects on the host. Secondary bile acids produced by intestinal bacteria are ligands for specific nuclear receptors, which regulate glucose, lipid, and drug metabolism in the liver. The present study aimed to clarify the effect of changes in secondary bile acids caused by antibiotic-induced dysbiosis on the host physiology, especially glucose, lipid, and drug metabolism. After oral administration of non-absorbable antibiotics for 5 days, decreased amounts of secondary bile acid-producing bacteria in faeces and a reduction in secondary bile acid [lithocholic acid (LCA) and deoxycholic acid (DCA)] levels in the liver were observed. Serum glucose and triglyceride levels were also decreased, and these decreases were reversed by LCA and DCA supplementation. Quantitative proteomics demonstrated that the expression levels of proteins involved in glycogen metabolism, cholesterol, bile acid biosynthesis, and drug metabolism (Cyp2b10, Cyp3a25, and Cyp51a1) were altered in the liver in dysbiosis, and these changes were reversed by LCA and DCA supplementation. These results suggested that secondary bile acid-producing bacteria contribute to the homeostasis of glucose and triglyceride levels and drug metabolism in the host, and have potential as therapeutic targets for treating metabolic disease.
Atomic structure and passivated nature of the Se-treated GaAs(111)B surface
Authors: Ohtake Akihiro , Goto Shunji , Nakamura Jun 
Published: January 2018

AbstractWe have systematically studied the atomic structure and electronic properties of the Se-treated GaAs(111)B surface using scanning tunneling microscopy, reflection high-energy electron diffraction, x-ray photoelectron spectroscopy, and first-principles calculations. We have found that Se atoms substitute $$\frac{3}{4}$$

3
4

monolayer of As atoms at the outermost layer of the ideal (111)B surface. Charge transfer from Se to As eliminates all of unsaturated dangling bonds, so that the surface is electronically stabilized, leaving no surface states in the mid-gap region.
Study of an Oxygen Supply and Oxygen Saturation Monitoring System for Radiation Therapy Associated with the Active Breathing Coordinator
Authors: Gong Guanzhong , Guo Yujie , Sun Xuemei , Wang Xiuying , Yin Yong , Feng David Dagan 
Published: January 2018

AbstractIn this study, we designed an oxygen supply and oxygen saturation monitoring (OSOSM) system. This OSOSM system can provide a continuous supply of oxygen and monitor the peripheral capillary oxygen saturation (SpO2) of patients who accept radiotherapy and use an active breathing coordinator (ABC). A clinical test with 27 volunteers was conducted. The volunteers were divided into two groups based on the tendency of SpO2 decline in breath-holding without the OSOSM system: group A (12 cases) showed a decline in SpO2 of less than 2%, whereas the decline in SpO2 in group B (15 cases) was greater than 2% and reached up to 6% in some cases. The SpO2 of most volunteers declined during rest. The breath-holding time of group A without the OSOSM system was significantly longer than that of group B (p < 0.05) and was extended with the OSOSM system by 26.6% and 27.85% in groups A and B, respectively. The SpO2 recovery time was reduced by 36.1%, and the total rest time was reduced by 27.6% for all volunteers using the OSOSM system. In summary, SpO2 declines during breath-holding and rest time cannot be ignored while applying an ABC. This OSOSM system offers a simple and effective way to monitor SpO2 variation and overcome SpO2 decline, thereby lengthening breath-holding time and shortening rest time.
Integrating modality-specific expectancies for the deployment of spatial attention
Authors: Mengotti Paola , Boers Frank , Dombert Pascasie L. , Fink Gereon R. , Vossel Simone 
Published: January 2018

AbstractThe deployment of spatial attention is highly sensitive to stimulus predictability. Despite evidence for strong crossmodal links in spatial attentional systems, it remains to be elucidated how concurrent but divergent predictions for targets in different sensory modalities are integrated. In a series of behavioral studies, we investigated the processing of modality-specific expectancies using a multimodal cueing paradigm in which auditory cues predicted the location of visual or tactile targets with modality-specific cue predictability. The cue predictability for visual and tactile targets was manipulated independently. A Bayesian ideal observer model with a weighting factor was applied to trial-wise individual response speed to investigate how the two probabilistic contexts are integrated. Results showed that the degree of integration depended on the level of predictability and on the divergence of the modality-specific probabilistic contexts (Experiments 1–2). However, when the two probabilistic contexts were matched in their level of predictability and were highly divergent (Experiment 3), higher separate processing was favored, especially when visual targets were processed. These findings suggest that modality-specific predictions are flexibly integrated according to their reliability, supporting the hypothesis of separate modality-specific attentional systems that are however linked to guarantee an efficient deployment of spatial attention across the senses.
Spin Hall-induced auto-oscillations in ultrathin YIG grown on Pt
Authors: Evelt M. , Safranski C. , Aldosary Mohammed , Demidov V. E. , Barsukov I. , Nosov A. P. , Rinkevich A. B. , Sobotkiewich K. , Li Xiaoqin , Shi Jing , Krivorotov I. N. , Demokritov S. O. 
Published: January 2018

AbstractWe experimentally study nanowire-shaped spin-Hall nano-oscillators based on nanometer-thick epitaxial films of Yttrium Iron Garnet grown on top of a layer of Pt. We show that, although these films are characterized by significantly larger magnetic damping in comparison with the films grown directly on Gadolinium Gallium Garnet, they allow one to achieve spin current-driven auto-oscillations at comparable current densities, which can be an indication of the better transparency of the interface to the spin current. These observations suggest a route for improvement of the flexibility of insulator-based spintronic devices and their compatibility with semiconductor technology.
Maize chlorotic mottle virus exhibits low divergence between differentiated regional sub-populations
Authors: Braidwood Luke , Quito-Avila Diego F. , Cabanas Darlene , Bressan Alberto , Wangai Anne , Baulcombe David C. 
Published: January 2018

AbstractMaize chlorotic mottle virus has been rapidly spreading around the globe over the past decade. The interactions of maize chlorotic mottle virus with Potyviridae viruses causes an aggressive synergistic viral condition - maize lethal necrosis, which can cause total yield loss. Maize production in sub-Saharan Africa, where it is the most important cereal, is threatened by the arrival of maize lethal necrosis. We obtained maize chlorotic mottle virus genome sequences from across East Africa and for the first time from Ecuador and Hawaii, and constructed a phylogeny which highlights the similarity of Chinese to African isolates, and Ecuadorian to Hawaiian isolates. We used a measure of clustering, the adjusted Rand index, to extract region-specific SNPs and coding variation that can be used for diagnostics. The population genetics analysis we performed shows that the majority of sequence diversity is partitioned between populations, with diversity extremely low within China and East Africa.