bluuebunny/crossref_metadata_2025_split huggingface dataset:
NELL-1 Associated Membranous Nephropathy: A case report of Lipoic Acid-Induced Nephrotic Syndrome
Authors: Rashid Saman , Ramamoorthy Lakshiya , Kannan Bharath Duraisamy Swami 
Published: September 2024

Abstract

Membranous nephropathy (MN) often arises from secondary causes such as hepatitis, HIV or heavy metal poisoning, but occasionally occurs without any identifiable underlying cause. Typically, more than 70% of cases are positive for phospholipaseA2 receptor 1(PLA2R1) and thromboplastin type 1 domain-containing protein 7 A (THSD7A). However, emerging evidence suggests associations with novel biomarkers. We present a case in which MN was associated with NELL-1 expression due to lipoic acid use (LA), highlighting the importance of identifying emerging biomarkers for MN diagnosis and timely initiation of appropriate treatment.
Clinical Trial Number: Not applicabale
Reduction of RAD23A extends lifespan and mitigates pathology in TDP-43 mice
Authors: kalb Robert , Guo Xueshui , Prajapati Ravindra , Chun Jiyeon , Byon Insuk , Gebis Kamil , Wang Yi-Zhi , Ling Karen , Dalton Casey , Blair Jeff , Hamidianjahromi Anahid , Bachmann Gemma , Rigo Frank , Jafar-nejad Paymaan , Savas Jeffrey , Lee Min , Sreedha Jemeen 
Published: September 2024

Abstract

Protein misfolding and aggregation are cardinal features of neurodegenerative disease (NDD) and they contribute to pathophysiology by both loss-of-functon(LOF) and gain-of-functon(GOF) mechanisms. This is well exemplified by TDP-43 which aggregates and mislocalizes in several NDDs. The depletion of nuclear TDP-43 leads to reduction in its normal function in RNA metabolism and the cytoplasmic accumulation of TDP-43 leads to aberrant protein homeostasis. A modifier screen found that loss of rad23 suppressed TDP-43 pathology in invertebrate and tissue culture models. Here we show in a mouse model of TDP-43 pathology that genetic or antisense oligonucleotide (ASO)-mediated reduction in rad23a confers benefits on survival and behavior, histological hallmarks of disease and reduction of mislocalized and aggregated TDP-43. This results in improved function of the ubiquitin-proteasome system (UPS) and correction of transcriptomic alterations evoked by pathologic TDP-43. RAD23A-dependent remodeling of the insoluble proteome appears to be a key event driving pathology in this model. As TDP-43 pathology is prevalent in both familial and sporadic NDD, targeting RAD23A may have therapeutic potential.
LLMscreen: A Python Package for Systematic Review Screening of Scientific Texts Using Prompt Engineering
Authors: Xia Ziqian , Ye Jinquan , Hu Bo , Qiang Qiqi , Debnath Ramit 
Published: September 2024

Abstract

Systematic reviews represent a cornerstone of evidence-based research, yet the process is labor-intensive and time-consuming, often requiring substantial human resources. The advent of Large Language Models (LLMs) offers a novel approach to streamlining systematic reviews, particularly in the title and abstract screening phase. This study introduces a new Python package built on LLMs to accelerate this process, evaluating its performance across three datasets using distinct prompt strategies: single-prompt, k-value setting, and zero-shot. The k-value setting approach emerged as the most effective, achieving a precision of 0.649 and reducing the average error rate to 0.4%, significantly lower than the 10.76% error rate typically observed among human reviewers. Moreover, this approach enabled the screening of 3,000 papers in under 8 minutes, at a cost of only $0.30—an over 250-fold improvement in time and 2,000-fold cost efficiency compared to traditional methods. These findings underscore the potential of LLMs to enhance the efficiency and accuracy of systematic reviews, though further research is needed to address challenges related to dataset variability and model transparency. Expanding the application of LLMs to other stages of systematic reviews, such as data extraction and synthesis, could further streamline the review process, making it more comprehensive and less burdensome for researchers.
Sysmtematic study of liver inflammation related genes in Human (Homo sapiens) and Chimpanzee (Pan troglodytes).
Authors: Shaukat Rizwan , Arshad Hafsa 
Published: September 2024

Abstract

This study employs computational analysis to identify and characterize liver inflammation-related genes in humans. Focusing on five genes including as mitogen-activated protein kinase 1 (MAPK1), integrin subunit alpha 2 (ITGA2), cyclin dependent kinase 2 (CDK2), interleukin 6 (IL6), and 2'-5'-oligoadenylate synthetase 2 (OAS2), the research includes gene sequence retrieval (NCBI), chromosomal mapping, gene structure prediction, motif analysis, protein-protein interaction networks, and gene ontology annotation. The analysis reveals that these genes are randomly distributed across chromosomes and are involved in key biological processes related to liver inflammation. Protein-protein interaction analysis identifies ITGA2 as a central gene with high interaction degrees. Gene ontology results show IL6 and OAS2 significant roles in the defense response to viruses. OSA2 –Hs gene showed more phylogeny with OSA2 –Pt while showed larger diversity with MAPK1-Hs visualized by phylogenetic. Expression analysis showed that CDK2 gene showed up-regulation in telencephalon (high expression).The findings offer insights into liver inflammation mechanisms and suggest potential biomarkers for early disease monitoring and therapeutic strategies. Further experimental validation is needed to confirm these computational predictions.
Evaluating Domain Coverage in Low-Resource Generative Chatbots: A Comparative Study of Open-Domain and Closed-Domain Approaches Using BLEU Scores
Authors: Soni Arpita , Arora Rajeev , Kumar Anoop , Panwar Dheerendra 
Published: September 2024

Abstract

Chatbots employ Natural Language Processing (NLP) and Artificial Intelligence (AI) to conduct automated chats and provide online assistance to users. Chatbots fall into two categories—generative models and retrieval models. E-commerce is the main application for retrieval-based chatbots—which provide pre-programmed responses and are easy to use with controlled interactions. While generative-based chatbots are more appropriate for research applications—they are more difficult to train and maintain. This is because they produce unique responses based on large conversational datasets. Therefore, in order to establish whether open-domain or closed-domain techniques work better—this study examines the domain coverage of low-resource generative chatbots. We compare their performance using Bilingual Evaluation Understudy (BLEU) scores in different domains to determine which method performs better.
Involvement of NADPH oxidase in the shedding of tissue factor-bearing vesicles from human monocytic cells exposed to ice-cold temperature
Authors: Nishioka Akira , Azma Toshiharu , Mio Yasushi 
Published: September 2024

Abstract

Recent rise in whole blood usage for traumatic hemorrhagic shock prompts reevaluation of leukocyte's impact on hemostatic function during cold storage. We investigated whether tissue factor (TF) production in human monocytic cells (THP-1) is influenced by cold storage or rewarming, employing mechanisms similar to apoptosis. We also explored the role of superoxide anion (·O2−) generated from NADPH oxidase (NOX) in TF production.
Methods: THP-1 cells incubated at 4°C for up to 24 h with/without test reagents were rewarmed at 37°C, and subject to flow cytometry. Cells were washed by centrifugation before the rewarming as required. TF in the supernatant of cells was also measured.
Results: TF and the proportion of apoptotic cells increased during cold storage for up to 24 h. TF increased at 1–2 h after cell lavage following the cold storage, indicating functional shedding of TF-bearing vesicles, not by leakage through the cell membrane due to apoptotic cell damage. TF-bearing vesicles shed from THP-1 cells were distinct from apoptotic vesicles. SOD and catalase inhibited TF production during cold storage, but only SOD suppressed TF production after cell lavage. Western blot analysis confirmed the recruitment of p47phox and p67phox into the cell membrane during cold storage, indicating involvement of ·O2− from NOX in TF-bearing vesicle shedding.
Conclusions: We found that extracellular addition of SOD successfully suppressed TF release from THP-1 cells exposed to cold, suggesting that ·O2− derived from NOX was involved in the TF release from human monocytic cells during both cold storage and rewarming.
Integrated urban wastewater system operation: Performance evaluation and sewer network's operation implications
Authors: Vasiliev Iulian , Luca Laurențiu , Barbu Marian , Vilanova Ramon , Caraman Sergiu , Meneses Benitez Montserrat 
Published: September 2024

ABSTRACT

The paper deals with the operation of the Integrated Urban Wastewater System as composed of the Sewer Network and associated Wastewater Treatment Plant. The performance of the integrated system is defined in terms of appropriate, selected, performance indicators of both subsystems. Both subsystems are connected and interact by a piping system. It is shown how the performance of the Integrated Urban Wastewater System is affected by various Sewer Network control scenarios: (1) only the Sewer Network outlet valve opening is controlled, (2) all the Sewer Network valves are controlled by a Model Predictive Control algorithm developed for minimizing the environmental impact of the subsystem and modified by constraining it in terms of Sewer Network outlet valve opening. More specific, it is proven that the Sewer Network outlet valve opening has an important role in the performances of the integrated system, and that already existing Sewer Network control strategies can be altered in such a way that they will enhance these performances.
A Quantitative Review of Un-licensed and Off-label Medicines Use in Children Aged 0-2 Years in the Private Sector in South Africa: Extent,
Challenges, and Implications
Authors: Mathevula Hlayiseka , Matlala Moliehi , Schellack Natalie , Orubu Samuel 
Published: September 2024

Background:
The global lack of suitable formulations for children leads to off-label
and unlicensed medicine use, posing significant risks of adverse effects. Understanding this usage
on a national level can help guide interventions for better formulations. This study aimed to measure
the prevalence of off-label and unlicensed medicines among children in South Africa's private
sector.

Methods:
The study used a point prevalence methodology to review medicine use in children
aged 0-2 years enrolled in a selected pharmaceutical benefit management company in South Africa
from January to June 2022. A sample size of 1055 prescriptions was calculated using a 90%
confidence interval, 50% prevalence rate, and 5% error margin. A systematic random sampling approach
selected every seventh entry from 91,973 total entries, resulting in a final sample size of
13,139. Data included patient age, number and characteristics of medicines, quantity, and indications.
Descriptive statistics analysed and reported the prevalence of unlicensed and off-label
medicine use.

Results:
Among the 13,139 prescribed medicines, 40% (5,246) were off-label or unlicensed, and
60% (7,893) were on-label. Of the off-label/unlicensed medicines, 16.85% (2,214) were unlicensed,
and 23.08% (3,032) were off-label. Methylprednisolone was the top off-label medicine,
probiotics were the top unlicensed, and the ICD10 code Z76.9 was the top diagnosis.

Conclusion:
The study found that 40% of children aged 0-2 years were prescribed unlicensed or
off-label medicines in South Africa's private healthcare sector between January and June 2022.
This suggests a widespread practice of off-label or unlicensed prescriptions in paediatric treatment
in the South African private sector.

result:
The sample contained 13,139 prescribed medicines. 40% (5,246) were used off-label or unlicensed, while 60% (7,893) were used on-label. Of the off-label/unlicensed, 16.85% (2,214) were unlicensed and 23.08% (3,032) were used off-label. The most commonly used off-label medicines were methylprednisolone (4%), cetirizine syrup (3%), and fluticasone propionate spray (3%). The most commonly used unlicensed medicines were probiotics (10%), multivitamin syrup (7%), and Hedera helix (5.0%). Cough and cold preparations (18%) were the most commonly dispensed WHO ATC category, followed by corticosteroids and dermatological preparations (10%). Z76.9 is the most common diagnosis (ICD10 code) linked to the use of prescription drugs off-label and unlicensed.
Dihydro-Cucurbitacin-E; a Secondary Metabolite of Curvularia lunata's Biotransformation as a Promising cPLA-2 and sPLA-2 Inhibitor in B[a]P-Induced Lung Toxicity
Authors: Abd El-Mongy Mahmoud , Hussein Mohammed Abdalla , Embabi Fotna Magdy , Roshdy Tamer , Salah Ahmed 
Published: September 2024

Background
Lung cancer is the most common cause of mortality, and its prevalence is rising quickly, making it a major global health concern. Numerous studies have indicated that benzo(a)pyrene [B[a]P] in cigarette smoke is the main cause of lung toxicity.

Objective
The study's goal was to apply Curvularia lunata NRRL 2178 in biotransformation cucurbitacin-E-glucoside to dihydro-cucurbitacin-E (DHCE). We characterized the isolated DHCE using 1H-NMR and 13C-NMR spectra. We extended our study to evaluate the cancer activity of DHCE against A-549 cells in vitro, as well as its lung protective activity against B[a]P-induced lung toxicity.

Methods
We incubated Curvularia lunata NRRL 2178 with cucurbitacin-E-glucoside DHCE for 14 days. We isolated and characterized the obtained metabolite, DHCE, using 1H-NMR and 13C-NMR techniques. We also evaluated the IC50 of the isolated DHCE against A-549 cells. On the other hand, we conducted in vivo studies to assess its lung protective effect against B[a]P-induced toxicity in mice.

Results
The results of the 1H-NMR and 13C-NMR experiments showed that the metabolite, DHCE, was found because it lacked two trans-olefinic protons (23 and 24) and the hydrogen atoms of the glucose moiety in the cucurbitacin-E-glucoside skeleton structure. The IC50 value of DHCE against A-549 cells is 38.87 µg/mL, respectively. The LD50 of DHCE was 930 mg/kg b.w. Giving DHCE (18.6 and 46.5 mg/kg b.w.) orally to mice that had been given B[a]P (20 mg/kg b.w.) every day for 30 days made their plasma total cholesterol (TC), triglycerides (TG), and high density lipoprotein-c (HDL-C) levels much better, as well as their lung reduced glutathione (GSH), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels, compared to mice that had been given B[a]P. On the other hand, oral administration of DHCE enhanced plasma interleukin-6 (IL-6) and nuclear factor (NF)-κB, as well as matrix metalloproteinases-2 (MMP-2) and MMP-12, in the lung of treated mice. On the other hand, administering DHCE to lung mice treated with B[a]P reduced the activity of crucial genes linked to lung inflammation, specifically cytosolic (cPLA2) and Secretory Phospholipase A2 (sPLA2). Furthermore, DHCE nearly normalized these effects in lung histoarchitecture.

Conclusion
The obtained biochemical, molecular biology, and histological results proved the lung protective activity of Curvularia lunata metabolite (DHCE) against B[a]P-induced lung toxicity in mice.
Mengendalikan penyakit tidak menular di Sleman Yogyakarta: studi kader pos pembinaan terpadu parikesit
Authors: Hidayana Alif Nur , Hendrastomo Grendi 
Published: September 2024

Penelitian mengkaji peran sosial kader posbindu Parikesit dalam upaya pencegahan dan pengendalian penyakit tidak menular di KapanewonKalasan. Dengan melakukan penelitian lapangan dengan pendekatan kualitatif melalui wawancara dengan perangkat desa, pimpinan dan anggota/kader posbindu dan warga masyarakat, kami mendokumentasikan program dan hambatan yang dilakukan dan dialami selama ini. Hasilpenelitian menunjukkan peran sosial posbindu Parikerit dalam (i) meningkatkan partisipasi masyarakat, (ii) memberikan edukasi dan motivasikepada masyarakat dan (iii) mendampingi dna menfasilitasi warga mengindari penyakit tidak menular. Namun kader-kader posbindu juga menghadapi hambatan, seperti ((i) kurangnya pengetahuan dan keterampilan kader, (ii) minimnya sumber dana, dan (iii) belum maksimalnyapemanfaatan posbindu oleh kelompok sasaran. Oleh karena itu, kader dituntut untuk meningkatkan pengetahuan dan ketrampilan dan perlunya inovasi program/kegiatan mencegah dan mengedalikan penyakit tidak menular di masyarakat.
Intensitas akses media digital dan partisipasi politik di Indonesia: data WVS7 2018
Authors: Bramasta Alvin Sandyka , Pierewan Adi Cilik 
Published: September 2024

Pada era digital, perangkat teknologi digunakan sebagai kebutuhan informasi dari media digital. Setiap orang memiliki kontrol pemilihan informasi yang ingin konsumsi menggunakan media digital sehingga konsumsi informasi politik melalui media digital dapat memicu partisipasi politik. Penelitian ini menggunakan metode kuantitatif dengan menggunakan data World Values Survey Gelombang 7 Indonesia 2018, yang jumlah respondennya sebanyak 3200 orang. Hasil penelitian menunjukkan email dan internet signifikan secara langsung memengaruhi partisipasi politik responden. Hasil temuan uji regresi dalam penelitian ini menjelaskan pengaruhnya terhadap partisipasi politik konvensional secara signifikan (nilai P: 0,000) dengan koefisien jalur 0,243, sedangkan pengaruh pada partisipasi non konvensional lebih signifikan (nilai P: 0,000) dengan koefisien jalur 0,355.
Konstruksi sosial tradisi Pesta Baratan Yayasan Lembayung Jepara
Authors: Lestari Khilma Ayu , Astutik Dwi , Purwanto Danang 
Published: September 2024

Penelitian mendeskripsikan konstruksi sosial tradisi Pesta Baratan di Yayasan Lembayung, Kalinyamatan, Jepara. Dengan menggunakan metode kualitatif deskriptif dengan pendekatan studi kasus berbasis teori Konstruksi Sosial dari Berger dan Luckman, Pesta Baratan yang merupakan tradisi lokal asli Jepara merupakan hasil konstruksi sosial dengan Yayasan Lembayung. Lembaga ini berperan dari mulai proses pembentukannya hingga pelestariannya. Momen Eksternalisasi dimulai dari adanya stock of knowledge tradisi baratan serta adanya penyesuaian dengan kondisi sosial kultural dan keagamaan masyarakat sekitar. Objektivasi terjadi ketika masyarakat dapat menerima Pesta Baratan sebagai realitas objektif melalui tahap habitualisasi dan legitimasi. Momen internalisasi terjadi ketika masyarakat memiliki pemaknaan baru terhadap Pesta Baratan. Yayasan Lembayung berperan penting di dalam tahap sosialisasi dan pelestarian Pesta Baratan di Jepara.The research describes the social construction of the Baratan Festival tradition at the Lembayung Foundation, Kalinyamatan, Jepara. Using a descriptive qualitative method with a case study approach based on Berger and Luckman's Social Construction theory, the Baratan Festival, which is a local tradition native to Jepara, is the result of social construction with the Lembayung Foundation. This institution plays a role from the process of its formation to its preservation. The moment of externalization starts with the stock of knowledge of western traditions and adjustments to the socio-cultural and religious conditions of the surrounding community. Objectivation occurs when society can accept the Baratan Party as an objective reality through the stages of habitualization and legitimation. The moment of internalization occurs when people have a new meaning for the Baratan Party. The Lembayung Foundation plays an important role in the socialization and preservation stages of the Baratan Festival in Jepara.
Investigating the Associations Between COVID-19, Long COVID, and Sleep Disturbances: Cross-Sectional Study
Authors: Shao Heng , Chen Hui , Xu Kewang , Gan Quan , Chen Meiling , Zhao Yanyu , Yu Shun , Li Yutong Kelly , Chen Lihua , Cai Bibo 
Published: September 2024

Abstract

Background
COVID-19 has not only resulted in acute health issues but also led to persistent symptoms known as long COVID, which have been linked to disruptions in sleep quality.

Objective
This study aims to investigate the associations between COVID-19, long COVID, and sleep disturbances, focusing on demographic, socioeconomic, and psychological factors among a Chinese population.

Methods
This cross-sectional study included 1062 participants from China. Demographic, socioeconomic, and clinical data were collected through web-based questionnaires. Participants were divided into 2 groups based on COVID-19 infection status: infected and noninfected. Within the infected group, participants were further categorized into those with long COVID and those without long COVID. Noninfected participants were included in the non–long COVID group for comparison. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI), while depression and anxiety were evaluated using the Patient Health Questionnaire-9 (PHQ-9) and the Generalized Anxiety Disorder-7 (GAD-7) scales, respectively. Multivariable linear regression was conducted to examine the associations between COVID-19, long COVID, and sleep quality, adjusting for demographic and psychosocial factors.

Results
COVID-19 infection was confirmed in 857 participants, with 273 of them developing long COVID. No significant sex disparities were observed in infection rates (P=.63). However, a marginal statistical difference was noted in the prevalence of long COVID among females (P=.051). Age was significantly associated with both infection rates (P<.001) and long COVID (P=.001). Participants aged 60‐70 years were particularly vulnerable to both outcomes. Sleep latency was significantly longer in the infected group (mean 1.73, SD 0.83) compared to the uninfected group (mean 1.57, SD 0.78; P=.01), and PSQI scores were higher (mean 8.52, SD 4.10 vs. 7.76, SD 4.31; P=.02). Long COVID participants had significantly worse sleep outcomes across all metrics (P<.001), except for sleep medication use (P=.17).

Conclusions
Our findings indicate that long COVID is strongly associated with significant sleep disturbances, while initial COVID-19 infection shows a more moderate association with sleep issues. Long COVID–related sleep disturbances were exacerbated by factors such as age, income, and chronic health conditions. The study highlights the need for targeted interventions that address the multifaceted impacts of long COVID on sleep, especially among vulnerable groups such as older adults and those with lower socioeconomic status. Future research should use longitudinal designs to better establish the temporal relationships and causal pathways between COVID-19 and sleep disturbances.