bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the Regulon of AphB and Its Essential Roles in LuxR and Exotoxin Asp Expression in the Pathogen Vibrio alginolyticus
Authors: Gao Xiating , Liu Yang , Liu Huan , Yang Zhen , Liu Qin , Zhang Yuanxing , Wang Qiyao 
Published: July 2017

ABSTRACT

In
Vibrio
species, AphB is essential to activate virulence cascades by sensing low-pH and anaerobiosis signals; however, its regulon remains largely unknown. Here, AphB is found to be a key virulence regulator in
Vibrio alginolyticus
, a pathogen for marine animals and humans. Chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) enabled the detection of 20 loci in the
V. alginolyticus
genome that contained AphB-binding peaks. An AphB-specific binding consensus was confirmed by electrophoretic mobility shift assays (EMSAs), and the regulation of genes flanking such binding sites was demonstrated using quantitative real-time PCR analysis. AphB binds directly to its own promoter and positively controls its own expression in later growth stages. AphB also activates the expression of the exotoxin Asp by binding directly to the promoter regions of
asp
and the master quorum-sensing (QS) regulator
luxR
. DNase I footprinting analysis uncovered distinct AphB-binding sites (BBS) in these promoters. Furthermore, a BBS in the
luxR
promoter region overlaps that of LuxR-binding site I, which mediates the positive control of
luxR
promoter activity by AphB. This study provides new insights into the AphB regulon and reveals the mechanisms underlying AphB regulation of physiological adaptation and QS-controlled virulence in
V. alginolyticus
.

IMPORTANCE
In this work, AphB is determined to play essential roles in the expression of genes associated with QS, physiology, and virulence in
V. alginolyticus
, a pathogen for marine animals and humans. AphB was found to bind directly to 20 genes and control their expression by a 17-bp consensus binding sequence. Among the 20 genes, the
aphB
gene itself was identified to be positively autoregulated, and AphB also positively controlled
asp
and
luxR
expression. Taken together, these findings improve our understanding of the roles of AphB in controlling physiological adaptation and QS-controlled virulence gene expression.
35: COMPARISON OF RECENT THERAPEUTIC PROCEDURES IN IMMATURE NECROTIC PERMANENT TEETH WITH CONVENTIONAL APEXIFICATION THERAPY: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Amirzade-Iranaq Mohammad Hossein , Masoumi S. M. R. 
Published: July 2017

Background and aims:The role of recent therapeutic procedures in regenerative endodontic and increasing the success rate is not defined clearly. The aim of this study was to compare recent therapeutic procedures in immature necrotic permanent teeth with conventional apexification.Methods:PubMed, Embase, Web of Science, Scopus and the Cochrane Library were searched and relevant studies were included by hand-searching and all of the included study restricted to English language. Type of study to be included was randomized controlled trials on animals.Data extraction:Searches resulted in the identification of 73 articles, and after removing duplicates and studies not meeting inclusion criteria like case reports and case series, 7 studies were selected quality assessment of the randomized controlled trials was executed by using the Critical Appraisal Skills Programme (CASP). Eventually 6 of these studies were selected for meta-analysis by Comprehensive meta-analysis version 2.Results:Among all detected studies 6 were included to meta-analysis and it shows increase in root length and dentinal wall thickness. Radiographic evaluation significantly shows this increase (Z=2.36, P=0.01, OR=4.71, 95% CI: 1.34–12.92, I=0%) in comparison of control and experimental groups. Heterogeneity was not detected (I<25%). The risk of bias in selected studies were low.Conclusion:Recent therapies in regenerative endodontics show high level of evidence on root length and dentinal wall thickness but more clinical evidence such as randomized clinical trials on human is needed.
42: DESIGNING A DASHBOARD FOR EVALUATION THE BUSINESS-IT ALIGNMENT STRATEGIES IN HOSPITALS ORGANIZATIONS
Authors: piri Zakieh , Raef Behnaz , moftian Nazila , dehghani Mohamad , khara Rouhallah 
Published: July 2017

Background and aims:Business-IT Alignment Evaluation is One of the most important issues that managers should monitor and make decisions about it .Dashboard software combines data and graphical indicators to deliver at-a-glance summaries of information for users to view the state of their business and quickly respond. The aim of this study was to design a dashboard to assess the business-IT alignment strategies for hospitals organizations in Tehran University of Medical Sciences.Methods:This is a functional-developmental study. Initially, we searched related databases (PubMed and ProQuest) to determine the key performance indicators of business-IT alignment for selecting the best model for dashboard designing. After selecting the Luftman model, the key indicators were extracted for designing the dashboard model. In the next stage, an electronic questionnaire was designed based on extracted indicators. This questionnaire sends to Hospital managers and IT administrators. Collected data were analyzed by Excel 2015 and displayed in dashboard page.Results:The number of key performance indicators was 39. After recognition the technical requirements the dashboard was designed in Excel. The overall business-IT alignment rate in hospitals was 3.12. Amir-aalam hospital has the highest business-IT alignment rate (3.55) and vali-asr hospital has the lowest business-IT alignment rat (2.80).Conclusion:Using dashboard software improves the alignment and reduces the time and energy compared with doing this process manually.
69: FAMILY PRESENCE DURING RESUSCITATION: REQUIRED EVIDENCE-BASED GUIDELINE DEVELOPMENT
Authors: Hassankhani Hadi , Haririan Hamidreza , Porter Joanne E. 
Published: July 2017

Background and aims:Family Presence during Resuscitation is considered an important issue, however remains controversial among clinicians implementation to practice. The aim of this study was to explore the lived experiences of resuscitation team members with the presence of patient's family during resuscitation in the cultural context of Iran.Methods:An Interpretative phenomenology was used to discover the lived experiences of the nurses and physicians of six Tabriz hospitals, Iran, with the FPDR. A total of twelve nurses and nine physicians were interviewed over a 6 month period. The interviews were audio recorded, semi-structured and were transcribed verbatim. Van Mannen's technique was used for data analysis.Results:Two main and ten sub-themes emerged including; Destructive presence (cessation of resuscitation, interference in resuscitation, disruption to resuscitation team's focus, argument with resuscitation team, and adverse mental image in family) and Supportive presence (trust in the resuscitation team, collaboration with resuscitation team, alleviating family's concern and settling their nerve, increasing family's satisfaction, and reducing conflict with resuscitation team members). The results also revealed that well trained and expert team members, don't have any stress in the presence of family during resuscitation.Conclusion:Participants stated that FPDR may work as a double-edged sword to family and resuscitation team, hurting, or, saving quality. It is thus recommended that guidelines be made in order to protect patients and family right's, while considering the positive, saving, edge of the phenomenon for hospitals.
Switch From Fetal to Adult
SCN5A
Isoform in Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Unmasks the Cellular Phenotype of a Conduction Disease–Causing Mutation
Authors: Veerman Christiaan C. , Mengarelli Isabella , Lodder Elisabeth M. , Kosmidis Georgios , Bellin Milena , Zhang Miao , Dittmann Sven , Guan Kaomei , Wilde Arthur A. M. , Schulze‐Bahr Eric , Greber Boris , Bezzina Connie R. , Verkerk Arie O. 
Published: July 2017

Background

Human induced pluripotent stem cell–derived cardiomyocytes (hi
PSC
‐
CM
s) can recapitulate features of ion channel mutations causing inherited rhythm disease. However, the lack of maturity of these cells is considered a significant limitation of the model. Prolonged culture of hi
PSC
‐
CM
s promotes maturation of these cells. We studied the electrophysiological effects of the I230T mutation in the sodium channel gene

SCN
5A

in hi
PSC
‐
CM
s generated from a homozygous (I230T
homo
) and a heterozygous (I230T
het
) individual from a family with recessive cardiac conduction disease. Since the I230T mutation occurs in the developmentally regulated “adult” isoform of

SCN
5A,

we investigated the relationship between the expression fraction of the adult

SCN
5A

isoform and the electrophysiological phenotype at different time points in culture.

Methods and Results

After a culture period of 20 days, sodium current (

I
N

a
) was mildly reduced in I230T
homo
hi
PSC
‐
CM
s compared with control hi
PSC
‐
CM
s, while I230T
het
hi
PSC
‐
CM
s displayed no reduction in

I
N

a
. This coincided with a relatively high expression fraction of the “fetal”

SCN
5A

isoform compared with the adult isoform as measured by quantitative polymerase chain reaction. Following prolonged culture to 66 days, the fraction of adult

SCN
5A

isoform increased; this was paralleled by a marked decrease in

I
N

a
in I230T
homo
hi
PSC
‐
CM
s, in line with the severe clinical phenotype in homozygous patients. At this time in culture, I230T
het
hi
PSC
‐
CM
s displayed an intermediate loss of

I
N

a
, compatible with a gene dosage effect.

Conclusions

Prolonged culture of hi
PSC
‐
CM
s leads to an increased expression fraction of the adult sodium channel isoform. This new aspect of electrophysiological immaturity should be taken into account in studies that focus on the effects of

SCN
5A

mutations in hi
PSC
‐
CM
s.
Longitudinal study of hippocampal volumes in heavy cannabis users
Authors: Koenders L , Lorenzetti V , de Haan L , Suo C , Vingerhoets WAM , van den Brink W , Wiers RW , Meijer CJ , Machielsen MWJ , Goudriaan AE , Veltman DJ , Yücel M , Cousijn J 
Published: July 2017

Background: Cannabis exposure, particularly heavy cannabis use, has been associated with neuroanatomical alterations in regions rich with cannabinoid receptors such as the hippocampus in some but not in other (mainly cross-sectional) studies. However, it remains unclear whether continued heavy cannabis use alters hippocampal volume, and whether an earlier age of onset and/or a higher dosage exacerbate these changes. Methods: Twenty heavy cannabis users (mean age 21 years, range 18–24 years) and 23 matched non-cannabis using healthy controls were submitted to a comprehensive psychological assessment and magnetic resonance imaging scan at baseline and at follow-up (average of 39 months post-baseline; standard deviation=2.4). Cannabis users started smoking around 16 years and smoked on average five days per week. A novel aspect of the current study is that hippocampal volume estimates were obtained from manual tracing the hippocampus on T1-weighted anatomical magnetic resonance imaging scans, using a previously validated protocol. Results: Compared to controls, cannabis users did not show hippocampal volume alterations at either baseline or follow-up. Hippocampal volumes increased over time in both cannabis users and controls, following similar trajectories of increase. Cannabis dose and age of onset of cannabis use did not affect hippocampal volumes. Conclusions: Continued heavy cannabis use did not affect hippocampal neuroanatomical changes in early adulthood. This contrasts with prior evidence on alterations in this region in samples of older adult cannabis users. In young adults using cannabis at this level, cannabis use may not be heavy enough to affect hippocampal neuroanatomy.
Magnetic resonance imaging in children presenting migraine with aura: Association of hypoperfusion detected by arterial spin labelling and vasospasm on MR angiography findings
Authors: Cadiot Domitille , Longuet Romain , Bruneau Bertrand , Treguier Catherine , Carsin-Vu Aline , Corouge Isabelle , Gomes Constantin , Proisy Maïa 
Published: July 2017

Objective A child presenting with a first attack of migraine with aura usually undergoes magnetic resonance imaging (MRI) to rule out stroke. The purpose of this study was to report vascular and brain perfusion findings in children suffering from migraine with aura on time-of-flight MR angiography (TOF-MRA) and MR perfusion imaging using arterial spin labelling (ASL). Methods We retrospectively included all children who had undergone an emergency MRI examination with ASL and TOF-MRA sequences for acute neurological deficit and were given a final diagnosis of migraine with aura. The ASL perfusion maps and TOF-MRA images were independently assessed by reviewers blinded to clinical data. A mean cerebral blood flow (CBF) value was obtained for each cerebral lobe after automatic data post-processing. Results Seventeen children were finally included. Hypoperfusion was identified in one or more cerebral lobes on ASL perfusion maps by visual assessment in 16/17 (94%) children. Vasospasm was noted within the intracranial vasculature on the TOF-MRA images in 12/17 (71%) children. All (100%) of the abnormal TOF-MRA images were associated with homolateral hypoperfusion. Mean CBF values were significantly lower ( P < 0.05) in visually hypoperfused lobes than in normally perfused lobes. Conclusion ASL and TOF-MRA are two totally non-invasive, easy-to-use MRI sequences for children in emergency settings. Hypoperfusion associated with homolateral vasospasm may suggest a diagnosis of migraine with aura.
Non-health care facility anticonvulsant medication errors in the United States
Authors: DeDonato Emily A , Spiller Henry A , Casavant Marcel J , Chounthirath Thitphalak , Hodges Nichole L , Smith Gary A 
Published: July 2017

Introduction: This study provides an epidemiological description of non-health care facility medication errors involving anticonvulsant drugs. Methods: A retrospective analysis of National Poison Data System data was conducted on non-health care facility medication errors involving anticonvulsant drugs reported to US Poison Control Centers from 2000 through 2012. Results: During the study period, 108,446 non-health care facility medication errors involving anticonvulsant pharmaceuticals were reported to US Poison Control Centers, averaging 8342 exposures annually. The annual frequency and rate of errors increased significantly over the study period, by 96.6 and 76.7%, respectively. The rate of exposures resulting in health care facility use increased by 83.3% and the rate of exposures resulting in serious medical outcomes increased by 62.3%. In 2012, newer anticonvulsants, including felbamate, gabapentin, lamotrigine, levetiracetam, other anticonvulsants (excluding barbiturates), other types of gamma aminobutyric acid, oxcarbazepine, topiramate, and zonisamide, accounted for 67.1% of all exposures. Conclusions: The rate of non-health care facility anticonvulsant medication errors reported to Poison Control Centers increased during 2000–2012, resulting in more frequent health care facility use and serious medical outcomes. Newer anticonvulsants, although often considered safer and more easily tolerated, were responsible for much of this trend and should still be administered with caution.
The home as ethos of caring: A concept determination
Authors: Hilli Yvonne , Eriksson Katie 
Published: July 2017

Background: Within nursing, the concepts of home and homelike have been used indiscriminately to describe characteristics of healthcare settings that resemble a home more than an institution. Objectives: The aim of this study was to investigate the concept of home ( hem in Swedish). The main questions were as follows: What does the concept of home entail etymologically and semantically? Of what significance is the meaning of the concept to caring science and nursing? Design and methods: This study had a qualitative design with a hermeneutical approach guided by Gadamer. Eriksson’s model of concept determination was partly used to determine the etymology and semantics, the essence and epistemic category of the concept of home. In this study, etymological dictionaries and 17 Swedish language dictionaries published between 1850 and 2001 were investigated. Ethical consideration: In all parts of this study, ethical guidelines have been followed concerning both gathering data from dictionaries and other sources and during the interpretation of these sources. Findings: The home, framed as the ethos of caring, can be drawn as a three-dimensional picture where the three dimensions have a common core, enclosed and inviolable. Symbolically, the picture of home can be seen as the ethos of the human being’s innermost room, the human being’s manner of being and the tone expressed in the external or abstract room where the human being lives and interacts with others. Conclusion: Based on the findings in this study, we conclude that home as ethos is an inner ethical dimension within the human being. Human beings who are in contact with their ethos, the self, feel at home and dare to follow the voice of their heart. Nurses who experience at-homeness have an ability to invite the patient into a caring relationship. The home and the feeling of being at home have significant meaning in terms of human beings’ health and well-being.
Outreach syncope clinic managed by a nurse practitioner: Outcome and cost effectiveness
Authors: Hamdan Mohamed H , Walsh Kathleen E , Brignole Michele , Key Jamie 
Published: July 2017

Introduction The purpose of this study was to assess the clinical and financial outcomes of a novel outreach syncope clinic. Methods We compared the clinical outcome of the Faint and Fall Clinic at the American Center (January–June 2016) with that of the University of Wisconsin Health and Clinics Faint and Fall Clinic (January 2013–December 2014). The American Center-Faint and Fall Clinic is run solely by a nurse practitioner, assisted by online faint-decision software and consultancy of a faint specialist through video-conferencing. Results Five hundred and twenty-eight consecutive patients were seen at the University of Wisconsin Hospital and Clinics-Faint and Fall Clinic and 68 patients at the American Center-Faint and Fall Clinic. The patients’ clinical characteristics were similar except for a lower age in the American Center patients (45 ± 18 vs 51 ± 22, p = 0.03). Overall, a diagnosis was made within 45 days in 70% (95% confidence interval 66–74%) of the University of Wisconsin Hospital and Clinics patients and 69% (95% confidence interval 58–80%) of the American Center patients, ( p = 0.9). A mean of 3.0 ± 1.6 tests per patient was used in the University of Wisconsin Hospital and Clinics group compared to 1.5 ± 0.8 tests per patient in the American Center group, p = 0.001. Over the six-month study period, the total revenue at the American Center was US$152,597 (contribution margin of US$122,393 plus professional revenue of US$30,204). The total cost of the nurse practitioner including benefits was US$66,662 ((US$98,466 salary/year + 35.4% benefits)/2). Total revenue minus expenses resulted in a net profit of US$85,935. Discussion A nurse practitioner-run outreach syncope-clinic equipped with online faint-decision software and consultancy of a faint specialist through vedio-conferencing is feasible and financially self-sustainable. It allows the dissemination of standardized high-quality syncope care to patients who have no immediate access to a tertiary teaching hospital.
Atmospheric Pressure and Abdominal Aortic Aneurysm Rupture: Results From a Time Series Analysis and Case-Crossover Study
Authors: Penning de Vries Bas B. L. , Kolkert Joé L. P. , Meerwaldt Robbert , Groenwold Rolf H. H. 
Published: July 2017

Background: Associations between atmospheric pressure and abdominal aortic aneurysm (AAA) rupture risk have been reported, but empirical evidence is inconclusive and largely derived from studies that did not account for possible nonlinearity, seasonality, and confounding by temperature. Methods: Associations between atmospheric pressure and AAA rupture risk were investigated using local meteorological data and a case series of 358 patients admitted to hospital for ruptured AAA during the study period, January 2002 to December 2012. Two analyses were performed—a time series analysis and a case-crossover study. Results: Results from the 2 analyses were similar; neither the time series analysis nor the case-crossover study showed a significant association between atmospheric pressure ( P = .627 and P = .625, respectively, for mean daily atmospheric pressure) or atmospheric pressure variation ( P = .464 and P = .816, respectively, for 24-hour change in mean daily atmospheric pressure) and AAA rupture risk. Conclusion: This study failed to support claims that atmospheric pressure causally affects AAA rupture risk. In interpreting our results, one should be aware that the range of atmospheric pressure observed in this study is not representative of the atmospheric pressure to which patients with AAA may be exposed, for example, during air travel or travel to high altitudes in the mountains. Making firm claims regarding these conditions in relation to AAA rupture risk is difficult at best. Furthermore, despite the fact that we used one of the largest case series to date to investigate the effect of atmospheric pressure on AAA rupture risk, it is possible that this study is simply too small to demonstrate a causal link.
A single-center retrospective study: Clinical features of different types of Budd–Chiari syndrome in Chinese patients in the Hubei area
Authors: Li Gangping , Huang Ying , Tang Shunyu , Song Yuhu , Liang Huimin , Liu Dehan , Yang Ling , Hou Xiaohua 
Published: July 2017

Background The characteristics and prevalence of Budd–Chiari syndrome in China remain unclear. This study aimed to analyze the clinical features of Budd–Chiari syndrome in Chinese patients in the Hubei area. Methods One-hundred and thirty patients with Budd–Chiari syndrome, admitted to Union Hospital from January 2002 to January 2011, were included in this retrospective study. Clinical features, laboratory data, imaging characteristics, and cumulative patency rates were analyzed. Results Of the 130 patients with Budd–Chiari syndrome, 77 were men (59.2%) and 53 women (40.8%). Budd–Chiari syndrome was more commonly associated with inferior vena cava block (56.9%, 74/130) than hepatic vein block (19.2%, 25/130) and combined inferior vena cava/hepatic vein block (23.9%, 31/130). The clinical features of Budd–Chiari syndrome varied based on the location of the obstruction. The incidence of bilirubin abnormality, elevated alkaline phosphatase, and γ-glutamyl peptide transferase levels was common in patients with Budd–Chiari syndrome. Liver injury was more severe in cases with combined inferior vena cava/hepatic vein block than in the other two types of Budd–Chiari syndrome. Color Doppler ultrasound imaging was better for the diagnosis of hepatic vein obstruction, while computed tomography and magnetic resonance imaging were superior in diagnosing inferior vena cava obstruction. The cumulative 1-, 5-, and 10-year patency rates were 97%, 69%, and 59%, respectively. Univariate analysis indicated that liver cirrhosis was an independent risk factor of recurrence. Conclusion The most prevalent type of Budd–Chiari syndrome is inferior vena cava obstruction in Chinese patients in the Hubei area. Different types of Budd–Chiari syndrome have diverse clinical and biochemical features, which may assist clinicians in diagnosing Budd–Chiari syndrome. Liver cirrhosis was found as an independent risk factor of recurrence.