bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract MP29: Preeclampsia Dysregulates The MicroRNA Profile Of Small Extracellular Vesicles From Endothelial Colony Forming Cells
Authors: Hall Eva , Alderfer Laura , Haneline Laura , Hanjaya-Putra Donny 
Published: October 2024

Umbilical cord blood contains an enriched population of endothelial colony forming cells (ECFCs), which are vital for
de novo
vasculogenesis and vessel homeostasis. Previously, our lab has shown that preeclampsia (PE), a hypertensive disorder of pregnancy seen after 20 weeks of gestation, causes perturbations in how the ECFCs function, including decreased vasculogenic potential, and could cause increased risks to the infant later in life. Developing methods to monitor and restore the changes in these cells could significantly improve fetal outcomes. One such method is monitoring small extracellular vesicles (sEVs), which contain a cargo of RNA, DNA, and proteins that can influence other cell’s function through paracrine signaling. By quantifying microRNA (miRNA) expression in the PREC sEVs, we aim to identify potential biomarkers and determine their ability to restore PREC functionality.

ECFCs from healthy (ECFCs) and preeclamptic (PRECs) pregnancies were isolated from cord blood. Three low passage (P4-5) and three high passage (P7-8) cell lines were used for each condition. The supernatant from each cell type was collected, filtered, and ultracentrifuged to isolate the sEVs. The miRNA isolated from the sEVs was quantified using the Nanostring nCounter. Results were assessed using nSolver for data normalization, R for differential expression, and MetaCore for pathway analysis. High and low passage ECFC sEVs were added to the media of the PRECs (1x10
8
sEVs/mL). After 24 hours of treatment, a tube formation assay was run.

There is significant dysregulation of miRNA in the PRECs compared to the ECFCs. Most of the miRNA were associated with proliferation, gene transcription, migration, and angiogenesis. In addition, there are some PE associated functions affected including endometrial remodeling, decidualization, and embryonic development. After treating the PRECs with ECFC exosomes of high and low passages, the PRECs had a more distinct network with clear loops and more branching compared to the untreated control.
PE causes a distinct miRNA profile in PRECs. This data suggests that the dysregulated profile could be used as biomarkers to assess the progression of PE as some miRNA was identified in high or low passage only as well as provide a method for earlier diagnosis. In addition, treatment with healthy sEVs improving PREC tube forming ability indicates their potential as a therapeutic for enhancing vascularization post-PE and improving perinatal outcomes.
Abstract P208: Chromophobe Renal Cell Carcinoma Presenting as Hypertension in a Young Adult: A Case Report
Authors: Al Kayed Hadeel , Alqatawneh Maryam , Maaita Mahmoud , Alsuradi Awni , Odaibat Husam 
Published: October 2024

Introduction:
Renal cell carcinoma (RCC) is a recognized but rare cause of secondary hypertension. Hypertension in RCC can arise through several mechanisms, including direct renin secretion by the tumor (renin-secreting RCC), the tumor creating a mass effect on renal blood supply, decreasing it and activating the RAAS, or the presence of adrenal tissue within the tumor. Given these mechanisms, it is essential to consider renal tumors in the differential diagnosis of secondary hypertension. This case reports uncontrolled hypertension as the presenting complaint in a young female with a chromophobe RCC.

Case Presentation:
A 32-year-old female presented with intermittent headache, fatigue, and uncontrolled hypertension despite adherence to standard antihypertensive treatment. Laboratory tests revealed serum aldosterone at 37.9 ng/dL and markedly elevated plasma renin activity at 11.7 ng/mL/hr, suggesting hyperreninemic aldosteronism as the cause of her hypertension.

A CT scan revealed a large, mildly enhancing mass in the upper pole of the left kidney, measuring 93 x 92 x 96 mm (Figure 1).
Nephrectomy was discussed with the patient as part of her treatment plan and as a potential means of treating her hypertension. The patient consented, and the surgery was performed. Microscopic examination confirmed a chromophobe RCC extending into the renal sinus.

Conclusion:
This case underscores the importance of considering RCC in the differential diagnosis of secondary hypertension, even in young adults. Early recognition and surgical intervention, such as nephrectomy, are crucial, as they may alleviate hypertension and potentially serve as an early indicator for tumor detection before further progression.
Abstract P212: Microinjection of the novel angiotensin, alamandine-(1-5) into insular cortex, increase blood pressure
Authors: MARINS Fernanda , Godoy Ana , Monteiro Adelson , Peliky Fontes Marco Antonio , Santos Robson 
Published: October 2024

It is well known that the renin-angiotensin system plays a critical role in cardiovascular control and hydro-electrolyte homeostasis. At least in some circumstances Alamandine (Ala) and Angiotensin-(1-7) can have selective actions such as reported in the insular córtex (IC). Hydrolysis of Angiotensin-(1-7) by angiotensin converting enzyme (ACE) can form the heptapeptide Angiotensin-(1-5) and recently our group demonstrated that hydrolysis of Ala by ACE can form Ala-(1-5). In this study we aimed to evaluate the cardiovascular effects produced by microinjection of Ala-(1-5) and Angiotensin-(1-5) into the rostral region of the posterior IC.
Urethane (1.4g/kg) anesthetized rats were instrumented for measurement of mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA). Unilateral microinjection of vehicle (0,9% NaCl, 100nL, n=4), Ala (40pmol/100nl, n=7), Ala-(1-5) (400pmol/100nl, n=7) or Angiotensin-(1-5) (400pmol/100nl, n=5) were made into IC (+1.5mm rostral to the bregma).
At the baseline, there was no difference between the groups (Vehicle: 85±2 mmHg and 388±5bpm, Ala: 83±9mmHg and 389±10bpm, Ala-(1–5): 91±6mmHg and 386±7bpm or Ang-(1-5): 96±9mmHg and 375±6bpm). Vehicle microinjection was performed as a control for nonspecific effects (ΔMAP:2±1mmHg, ΔHR:4±2bpm, ΔRSNA:3±1%). Ala microinjection into IC, confirmed our previous data showing a long-lasting (18±3min) increase in MAP (Δ=14±2mmHg), and this effect was associated with a significant increase in HR (Δ=32±4bpm) and in RSNA (Δ=36±6%). These responses were elicited immediately after Ala microinjection.
When compared with vehicle and, very different from what was observed with Ala, microinjection of Ala-(1–5) evoked a significant increase in blood pressure without altering RSNA or HR (ΔMAP:22±2mmHg, ΔHR:-1±3bpm, ΔRSNA:1±3%). Differently from Ala evoked responses, the effects of Ala-(1–5) had a shorter duration (5.88±1.9min) without delay to beginning the effects. On other hand, microinjection of Angiotensin-(1-5) did not elicited autonomic and cardiovascular effects (ΔMAP:-1±3mmHg, ΔHR:-2±2bpm, ΔRSNA:2±3%).
In conclusion, these data showed that the IC is a brain site that has unique effects of Ala (increase in MAP, HR and RSNA) and Ala-(1-5) (increase in MAP only) contrasting with the essentially absent effects of Angiotensin-(1-7) and Angiotensin-(1-5). These findings will contribute to extend our understanding of the brain renin-angiotensin system.
Abstract P374: Systolic blood pressure burden better predicts tau accumulation than a one-time measurement
Authors: Manchineella Sushruth , Wang Xiuyuan , Butler Tracy , li yi , Chiang Gloria , Pahlajani Silky , Zhou Liangdong , de Leon Mony , Glodzik Lidia 
Published: October 2024

In our past work, our group identified a significant relationship between uncontrolled hypertension and greater accumulations of total-tau and phospho-tau181 in cerebrospinal fluid over time. Here, we tested whether blood pressure is related to brain tau deposition assessed using MK-6240 PET. Blood pressure is dynamic in nature, making it difficult to draw conclusions about their risk from a single measurement. To address this issue, different measurement methods have surfaced to better utilize blood pressure as a predictor of disease pathology. Previous studies have found cumulative diastolic blood pressure to increase cardiovascular disease stratification beyond that of a single diastolic blood pressure measurement.
In this project, we studied another method of quantifying blood pressure. We examined whether systolic blood pressure (SBP) burden was better related to tau accumulation than a one-time BP measurement taken at the time of PET scan. SBP load was defined as the percentage of SBP values >=140 mmHg taken in the period spanning from 6 months before tau imaging to 6 months after. SUVR (standardized uptake value ratio) was created using the cerebellar gray matter as the reference region. We assessed SUVR in Braak regions 1-6. Pearson correlation was used to test the associations between the variables of interest.
We studied cognitively healthy subjects from a brain aging research center (n=72, 40% women, 60% with hypertension). Medians of our group were 72 years of age (interquartile range (IQR) 10.6), education of 18 years (IQR 2), and 8 BP measurements (IQR 14). While one-time SBP measurements and tau were not related, SBP load was progressively correlated with SUVR across Braak stages. In the entire group, the correlations ranged from -0.18 (p = 0.133) in Braak region 1 to 0.19 (p = 0.114) in Braak region 3 to 0.24 (p = 0.042) in Braak region 6. In the hypertensive group, these correlations were stronger and ranged from -0.06 (p = 0.713) in Braak region 1 to 0.38 (p = 0.012) in Braak region 3 to 0.50 (p < 0.001) in Braak region 6.
We show that SBP load instead of singular SBP measurements is a stronger predictor of tau accumulation. Furthermore, stronger associations with brain regions known to be affected in more advanced stages suggest that blood pressure control is more critical to tau progression than initial deposition.
Abstract P377: Short-Chain Fatty Acids Improve Cardiac Function in a Hypertensive Rat Model of Heart Failure
Authors: Tagiyeva Nurana , Uhrbach Alisa , Kedziora Sarah , Forslund Sofia Kirke , Sawamiphak Suphansa , Dechend Ralf , Haase Nadine , Mueller Dominik 
Published: October 2024

Hypertension-induced ventricular remodeling contributes significantly to the progression of heart failure (HF). The global increase in HF prevalence results in heightened cardiovascular morbidity and mortality, yet treatment options remain limited. The gut microbiota ferments indigestible dietary fibers, producing essential metabolites such as short-chain fatty acids (SCFAs), amino acids metabolites. We hypothesize that direct oral administration of SCFAs modulates mucosal immune cells, thereby ameliorating the heart failure with preserved ejection fraction (HFpEF) phenotype in the double-transgenic rat (dTGR) model.
Four-week-old male rats, double-transgenic for human renin and angiotensinogen (dTGRs), were utilized. These dTGR rats exhibit classical symptoms of HFpEF, including hypertension, inflammation, and fibrosis in the heart and kidneys.
The rats were randomized into five experimental groups: healthy non-transgenic vehicle-treated controls (wild type), vehicle-treated dTGR, and dTGR treated with either acetate, propionate, or butyrate. Following a 3-week treatment period, comprehensive phenotyping was conducted, including echocardiography, Millar-tip catheterization, gene expression analysis, and histological examination.
SCFA administration improves diastolic function, as evidenced by alterations in the end-diastolic pressure-volume relationship measured via Millar-tip catheter. Notably, butyrate treatment had the most pronounced effect on alleviating diastolic dysfunction and reducing blood pressure. Additionally, gene expressions such as atrial natriuretic peptide, connective tissue growth factor, and myosin heavy chain alpha and beta showed improvement in SCFA-treated dTGR HFpEF rats compared to vehicle-treated counterparts. Furthermore, SCFA treatment ameliorated cardiac inflammation.
Our data suggest that SCFAs, particularly butyric acid, enhance hypertensive diastolic dysfunction and cardiac gene expression in dTGR rats, underscoring their therapeutic potential in HF.
Abstract P412: Pulmonary Hypertension in Patients with Preeclampsia: Prevalence, Predictors and Associated In-Hospital Adverse Events
Authors: Hamlet Casey , Afriyie Felix , Elkattawy Omar , Carrillo Gabriela , Folk Sarah , Patel Neha , Gardin Julius 
Published: October 2024

Pulmonary hypertension (PH) is prevalent pregnant women, but there is a lack of research into its co-occurence with preeclampsia. Our study aimed to characterize and evaluate the real-world prevalence, predictors and outcomes of PH in preeclamptic patients
Data were obtained from the National Inpatient Sample database from 2016 to 2019.Primary outcomes assessed were baseline and hospital admission characteristics and comorbidities for patients with preeclampsia with or without PH, outcomes for preeclamptic patients with PH, and predictors of mortality.
Of 255,513 preeclamptic patients, 497 (.194%) developed PH. Patients with PH had a significantly elevated likelihood of having IDA (9.7% vs 2.6%, p<.001), CHF (25.5% vs 0.4%, p<.001), pulmonary disease (17.7% vs 7.6%, p<.001), coagulopathy (2.0% vs 0.8%, p=.002), T2DM (7.2% vs 2.8%, p<.001), hypertension (0.8% vs 0.3%, p=.014), alcohol abuse (1.4% vs 0.1%, p<.001), liver disease (2.0% vs 0.4%, p<.001), atrial fibrillation (0.6% vs 0.1%, p<.001), hypothyroidism (7.0% vs 4.6%, p=.01), CAD (1.8% vs 0.1%, p<.001), OSA (8.7% vs 0.7%, p<.001), opioid use (1.8% vs 0.9%, p=.026), obesity (33.2% vs 19.4%, p<.001), and ESRD (0.8% vs 0.0%, p<.001). Univariate analysis showed that PH in preeclamptic patients was associated with higher rates of cardiac arrest (0.8% vs 0.0% p<.001), permanent pacemaker (1.4% vs 0.1% p<.001), ventricular fibrillation (0.2% vs 0.0% p<.001), angioplasty (0.8% vs 0.0% p<.001), PE (1.0% vs 0.0% p<.001), shock after delivery (1.2% vs 0.1% p<.001), PPH (10.1% vs 7.3% p=.017), right heart catheterization (2.0% vs 0.0% p<.001), left heart catheterization (0.6% vs 0.0% p<.001), cardiogenic shock (1.0% vs 0.0% p<.001), medical ventilation (3.6% vs 0.1% p<.001), vasopressor use (1.2% vs 0.1%, p<.001), STEMI (0.2% vs 0.0%, p<.001), and NSTEMI (1.2% vs 0.0% p<.001). Predictors of mortality included age at admission (OR 1.06, 95% CI 1.0–1.1, p<.05), Asian and Pacific Islander race (OR 4.89, 95% CI 1.9–12.7 p<0.05), CHF (OR 19.41 , 95% CI –, p<.05), coagulopathy (OR 19.4, 95% CI 6.4–58.8 p<.05), liver disease (OR 6.5, 95% CI 2.0–21.3 p<.05), eclampsia (OR 17.3, 95% CI 5.3–55.9 p<.05), and HELLP syndrome (OR 6.2, 95% CI 2.8–13.5 p<.05).
PH in preeclampsia is associated with an increased risk of maternal mortality, heart failure, arrhythmia, pulmonary embolism. The present study found age at admission, AAPI race, CHF, coagulopathy, liver disease, eclampsia, and HELLP syndrome to be predictors of mortality.
Botanists voted to remove offensive names; will zoologists follow?
Authors: ZHANG ZHI-QIANG 
Published: October 2024

The Nomenclature Section of the XX International Botanical Congress (IBC) was held from 15 to 19 July 2024, with 173 registered delegates in Madrid plus 219 additional attendees participating online (Gostel et al. 2014). It deliberated 433 proposals to amend the International Code of Nomenclature for algae, fungi, and plants (Turland et al. 2018, the Shenzhen Code). Among the decisions, one received significant media attention and was featured in top journals Science (Ortega & Stokstad 2024) and Nature (Callaway 2024): the removal of the letter c and the second f (if applicable) from 301 racially offensive names that include caf[e]r- and caff[e]r- (Smith & Figueiredo 2021). This proposal, along with others on offensive names (e.g., Hammer & Thiele 2021), received heated debates in Megataxa (Pethiyagoda 2023; Smith & Figueiredo 2023) and other journals (e.g., Jiménez-Mejías et al. 2024, with summaries of debates prior to this paper).
Influence of vascular graft type for ascending aorta replacement on the early postoperative outcome
Authors: Chigri Alina , Gerfer Stephen , Horatiu Coman , Krasivskyi Ihor , Luehr Maximilian , Djordjevic Ilija , Wahlers Thorsten , Strauch Justus , Haldenwang Peter 
Published: October 2024

Background Aim of this study was to find out if the type of vascular prosthesis used, especially collagen impregnated polyester versus gelatin impregnated woven fabric graft, has any impact on the early postoperative bleeding rate, blood product consumption and re-thoracotomy rate in isolated ascending aortic surgery. Methods n = 46 consecutive patients who received a supra-commissural replacement of the ascending aorta between 01/2016 - 01/ 2021 were included in this retrospective single-center study. The underlying pathology was an aortic aneurysm in 36 (81 %) and/or an acute type A aortic dissection (ATAAD) limited to the ascending aorta in 7 (15 %) and/or a penetrating aortic ulcer (PAU) with intramural hematoma in 6 (13 %) patients. According to the type of vascular graft used, the cohort was divided as follows: 25 patients (54%) received a double velour woven, collagen impregnated polyester graft (Hemashield, Getinge; CI-Group) whereas in 21 patients (46 %) a gelatin impregnated woven fabric graft was used (Gelweave, Vascutek / Terumo; GI-group). As primary endpoints class 3 bleeding according to the Valve Academic Research Consortium (VARC3) criteria and freedom from re-intervention were assessed. As secondary endpoints, 30-day mortality and stroke were defined. Results Preoperative risk assessment (EuroSCORE II), gender-, BMI-stratification and NYHA-classification as well as mean CPB-times (114 ± 44 min vs 110 ± 48 min) and aortic cross-clamp times (71 ± 28 min vs 66 ± 30 min) were similar in both groups. Bleeding, measured by drainage volume output within the first postoperative 24 h (480 ± 426 mL vs 389 ± 169 mL), erythrocytes concentrate consumption (2,4 vs 2,3) and similar re-thoracotomy rates (4 vs 4.7 %) showed no difference between groups. 30- day mortality (12 vs 5 %; p = 0.614) and stroke rates (4 vs 9.5; p = 0,4) showed no differences between groups. Conclusions Regarding postoperative bleeding no difference were seen between the two graft types. Long-term follow-up and larger prospective randomized studies are requested to prove these findings.
Reduced lipoprotein (a) in patients with severe hypertriglyceridaemia
Authors: Jacob Erin O. , McIntyre Adam D. , Wang Jian , Hegele Robert A. 
Published: October 2024

Objective To investigate the relationship between plasma lipoprotein (a) (Lp[a]) and lipid profiles in patients with severe hypertriglyceridaemia (HTG). Methods This case–control study undertook a retrospective chart review of patients from the Lipid Genetics Clinic at London Health Sciences Centre in Ontario, Canada. Plasma Lp(a) was compared between patients with severe HTG and healthy normolipidaemic control subjects. Severe HTG was defined by plasma triglycerides (TG) ≥ 10 mmol/l. Pairwise correlations between Lp(a), TG, apolipoprotein B (apo B) and non-high-density lipoprotein cholesterol (non-HDL-C) were evaluated. Results This study reviewed 4400 patients and identified 154 patients with severe HTG, which were compared with 272 control subjects. The median Lp(a) was significantly lower in patients with severe HTG compared with control subjects (5.0 versus 10.2 mg/dl, respectively). No correlation was observed between Lp(a) and TG or non-HDL-C. Lp(a) and apo B were modestly correlated in patients with severe HTG ( r = 0.235) and control subjects ( r = 0.175). There were no significant differences between the genetic subgroups of patients with severe HTG. Conclusions Patients with severe HTG have lower plasma Lp(a) than normolipidaemic control subjects. The basis for this relationship is not immediately apparent but is hypothesis-generating and warrants further investigation.
Summer Camp Clinical Placements in Young Families Nursing: An Interpretive Description Study
Authors: Williams Claire , Gordon Renée , Richard Emily 
Published: October 2024

Purpose Obtaining meaningful clinical experiences with paediatric and perinatal (young families) populations is increasingly challenging for nursing programs. Care for this population has largely moved to outpatient and tertiary settings. Therefore, a current trend is to use non-traditional clinical settings. While summer camps offer rich learning experiences for nursing students, they are seldom used as clinical placements. Faculty at an Atlantic Canadian university sought a novel way to engage students in young families’ learning by partnering with overnight summer camps, staffed by counsellors, camp administrators, and on-site nurses. Campers included those with lived experience of cancer, diabetes, physical and developmental challenges, and socioeconomic challenges. This study assesses how young families course outcomes were met by an innovative clinical experience within a Bachelor of Nursing program and describes the perceptions and experiences of those involved. Methods Study participants included students (n = 4), camp directors (n = 3), a camp nurse (n = 1), and the clinical educator (n = 1). Data collection involved semi-structured interviews and a focus group. Interpretive description methodology was used to identify themes and patterns related to overarching research questions. Findings Students met the outcomes and overall participant perceptions and experiences were positive. However, some participants shared constructive critiques for future consideration. Conclusions Nursing students who completed a young families clinical placement at summer camps met course outcomes, and those involved reported both positive experiences and constructive critiques for future consideration.
Enhanced hydrophobicity of polyurethane coating for steel protection through replication and nanomaterial integration
Authors: Soon Li Jie , Abdullah Tuti Katrina , Mahamad Husin Amira Sofea , Ahmad Zubir Syazana 
Published: October 2024

Polyurethanes (PU) are widely used in corrosion protection, anti-biofouling coatings, and self-cleaning glass, yet their low hydrophobicity poses durability challenges. This study addresses this by enhancing PU coatings’ hydrophobicity by refining surface roughness and forming tailored porous structures. Using drop, spin, dip, and spray coating techniques, hydrophobic PU coatings were fabricated on 304 steel plates. To further enhance hydrophobicity, 0.5 w/v% graphene nanoplatelets (GNP) were introduced into the coatings. Additionally, stainless-steel 500 mesh was incorporated to create patterned surfaces, increasing surface roughness and tailoring the porous structure. The analysis included optical and scanning electron microscopy, water contact angle (WCA) measurements, Fourier transform infrared spectroscopy, electrochemical corrosion tests, and atomic force microscopy. Incorporating GNP notably increased WCA by 20–23° and reduced corrosion rates in a 3.5% NaCl solution. Likewise, applying stainless-steel mesh improved surface roughness, raising WCA by 3-8° and decreasing corrosion rates. The study observed changes in porous structure, with pore size reduction correlating with increased roughness and decreased corrosion rates upon mesh application and GNP addition. This comprehensive examination highlights the potential of PU coatings for various applications and underlines the importance of tailored porous structures in enhancing their performance and versatility. Spin coating emerged as the optimal technique, yielding uniform, defect-free coatings with the highest WCA.