bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meiosis as a mechanism for epigenetic reprogramming and cellular rejuvenation
Authors: Berger Frédéric 
Published: October 2024

ABSTRACT
Meiosis is a hallmark of sexual reproduction because it represents the transition from one life cycle to the next and, in animals, meiosis produces gametes. Why meiosis evolved has been debated and most studies have focused on recombination of the parental alleles as the main function of meiosis. However, 40 years ago, Robin Holliday proposed that an essential function of meiosis is to oppose the consequence of successive mitoses that cause cellular aging. Cellular aging results from accumulated defective organelles and proteins and modifications of chromatin in the form of DNA methylation and histone modifications referred to collectively as epigenetic marks. Here, recent findings supporting the hypothesis that meiosis opposes cellular aging are reviewed and placed in the context of the diversity of the life cycles of eukaryotes, including animals, yeast, flowering plants and the bryophyte Marchantia.
Adapting to change: insights from new organisms in cell and developmental biology
Authors: Lemke Steffen , Albright Ashley , Guse Annika 
Published: October 2024

ABSTRACT
We are living in an era of environmental change with undeniable parallels with past mass extinctions. To improve our understanding of planetary health and resilience, we must expand our research beyond traditional lab models. Forecasting the future of biological diversity relies on extrapolation of past trends, which necessitates the study of a wider range of biological systems. The ‘Unconventional and Emerging Experimental Organisms for Cell and Developmental Biology’ meeting, which took place in Dorking, UK, in September 2023, emphasized the importance of this broader approach. Discussions centered on evolutionary innovation, robustness and diversity, underscoring the need for broader taxon sampling and novel experimental models to address current and future challenges.
Erythroblast enucleation at a glance
Authors: Newton Lucas M. , Fowler Velia M. , Humbert Patrick O. 
Published: October 2024

ABSTRACT
Erythroid enucleation, the penultimate step in mammalian erythroid terminal differentiation, is a unique cellular process by which red blood cells (erythrocytes) remove their nucleus and accompanying nuclear material. This complex, multi-stage event begins with chromatin compaction and cell cycle arrest and ends with generation of two daughter cells: a pyrenocyte, which contains the expelled nucleus, and an anucleate reticulocyte, which matures into an erythrocyte. Although enucleation has been compared to asymmetric cell division (ACD), many mechanistic hallmarks of ACD appear to be absent. Instead, enucleation appears to rely on mechanisms borrowed from cell migration, endosomal trafficking and apoptosis, as well as unique cellular interactions within the microenvironment. In this Cell Science at a Glance article and the accompanying poster, we summarise current insights into the morphological features and genetic drivers regulating the key intracellular events that culminate in erythroid enucleation and engulfment of pyrenocytes by macrophages within the bone marrow microenvironment.
Gut microbiota are involved in leptin-induced thermoregulation in the Mongolian gerbil (Meriones unguiculatus)
Authors: Tang Liqiu , Lv Jinzhen , Zhang Xueying , Wang Chen-Zhu , Wang Dehua 
Published: October 2024

ABSTRACT
Leptin is a hormone that is secreted by adipocytes and may promote energy expenditure by increasing thermogenesis. Our previous studies have shown that thermo-transient receptor potentials (thermo-TRPs) and gut microbiota are associated with thermoregulation in Mongolian gerbils, which are characterized by relative high serum leptin concentrations. Here, we tested whether leptin can stimulate non-shivering thermogenesis (NST) in Mongolian gerbils, and whether thermo-TRPs and gut microbiota are involved in leptin-induced thermogenesis. First, gerbils were given acute leptin treatment (ALT) with different doses. Results showed that ALT significantly increased the body temperature of gerbils and changed the composition of gut microbiota. Moreover, ALT groups showed a trend towards increased expression of uncoupling protein 1 (UCP1) in brown adipose tissue (BAT). Then, we investigated the effect of chronic leptin treatment (CLT) on gerbils. Surprisingly, CLT did not affect gerbils' food intake and body mass, but it significantly increased the body temperature at the end. Further, CLT did not affect the expression of thermogenic markers in BAT, white adipose tissue (WAT) or skeletal muscle. However, CLT increased the expression of leptin receptors and TRPV2 in the small intestine and affected the composition of gut microbiota. Together, our data suggest leptin may increase body temperature by regulating gut microbiota. In conclusion, serum hyperleptin in Mongolian gerbils is beneficial for adapting to cold environments, and TRPV2 and gut microbiota are involved.
Elevated Plasma IL-6 Coincides with Activation of STAT3 in PBMC After Acute Resistance Exercise
Authors: TSAI SHUN-HSI , CHENG HAO-CHIEN , LITTLE JONATHAN P. , ISLAM HASHIM , LIU HUNG-WEN 
Published: October 2024

ABSTRACT

Introduction
Changes in plasma concentrations of anti-inflammatory cytokines, such as interleukin-6 (IL-6) and IL-10, after acute resistance exercise (RE) have been widely explored. Whether observed changes in plasma cytokine concentration correspond to the activation of anti-inflammatory signaling pathways in immune cells after acute RE is unknown. This study aimed to determine if changes in plasma cytokines after acute RE resulted in the activation of anti-inflammatory signaling pathways in peripheral blood mononuclear cells (PBMC).

Methods
Healthy young males (N = 16; age = 23.5 ± 2.7 yr; BMI = 22.4 ± 1.7 kg·m−2) participated in a single session of whole-body RE (4 sets of 4 different exercises at 70% 1-repetition maximum with the last set to failure) and a sedentary control (CON) condition in a randomized crossover design. Blood samples were collected at several time points before and after the exercise bout.

Results
Higher plasma IL-6, IL-10, and IL-1 RA concentrations were observed after RE compared with CON. Phosphorylation of STAT3 and protein expression of SOCS3 in PBMC were increased in RE compared with CON. The elevation of plasma IL-6, but not IL-10, coincided with the activation of STAT3 signaling in PBMC.

Conclusions
These results highlight a potential mechanism by which RE may exert anti-inflammatory actions in circulating immune cells.
Modulators of Energy Expenditure Accuracy in Adults with Overweight or Obesity: E-MECHANIC Secondary Analyses
Authors: MATTHEWS RACHEL , HÖCHSMANN CHRISTOPH , ERICKSON MELISSA L. , DORLING JAMES L. , SPIELMANN GUILLAUME , JOHANNSEN NEIL M. , CHURCH TIMOTHY S. , MARTIN CORBY K. 
Published: October 2024

ABSTRACT

Purpose
American College of Sports Medicine (ACSM) metabolic equations are used to estimate energy expenditure (EE) of physical activity and prescribe aerobic exercise to meet EE requirements. Limited evidence supports their accuracy in sedentary adults with overweight or obesity during controlled exercise interventions. The purpose of this study was to compare EE estimated by the ACSM walking equation versus EE measured by indirect calorimetry during a 24-wk aerobic exercise intervention, and identify potential modulators for their accuracy.

Methods
Data from the exercising groups (8 or 20 kcal·kg body weight−1·wk−1) of the E-MECHANIC study were utilized in this ancillary analysis (N = 103). Every 2 wk for the initial 8 wk and monthly thereafter, EE was measured via indirect calorimetry during absolute (2 mph, 0% grade) and relative (65%–85% peak oxygen uptake (V̇O2peak)) workload exercise. Resting metabolic rate, V̇O2peak, and body composition were assessed at baseline and follow-up. An EE offset factor (EOF) was calculated to express measured EE as a percentage of the estimated EE at each workload (EOF < 100% represents an overestimation of ACSM estimated EE).

Results
The accuracy of the equation decreased with increasing exercise workload (0.44%, 9.2%, and 20.3% overestimation at absolute, relative, and maximal workloads, respectively, at baseline) and overestimation of EE was greater after the exercise intervention. Furthermore, race, sex, age, fat mass, and V̇O2peak were identified as modulators for equation accuracy. Greater overestimation of EE was observed in Black compared with White females, particularly at lower exercise workloads.

Conclusions
These findings support future efforts to improve the accuracy of metabolic equations, especially in diverse populations. Researchers should account for exercise efficiency adaptations when using metabolic equations to prescribe exercise precisely.
Long-term functional outcome of limb-sparing surgery for paediatric bone sarcoma around the knee
Authors: Sekita Tetsuya , Asano Naofumi , Kobayashi Hiroshi , Yonemoto Tsukasa , Kobayashi Eisuke , Ishii Takeshi , Kawai Akira , Nakayama Robert 
Published: October 2024

AimsSurgical limb sparing for knee-bearing paediatric bone sarcoma is considered to have a clinically significant influence on postoperative function due to complications and leg-length discrepancies. However, researchers have not fully evaluated the long-term postoperative functional outcomes. Therefore, in this study, we aimed to elucidate the risk factors and long-term functional prognosis associated with paediatric limb-sparing surgery.MethodsWe reviewed 40 patients aged under 14 years who underwent limb-sparing surgery for knee bone sarcoma (15 cases in the proximal tibia and 25 in the distal femur) between January 2000 and December 2013, and were followed up for a minimum of five years. A total of 35 patients underwent reconstruction using artificial materials, and five underwent biological reconstruction. We evaluated the patients’ postoperative complications, survival rate of reconstruction material, and limb, limb function, and leg-length discrepancy at the final follow-up, as well as the risk factors for each.ResultsComplications were observed in 55% (22/40) of patients. The limb survival and reconstruction material rates at five and ten years were 95% and 91%, and 88% and 66%, respectively. Infection was the only risk factor in both survivals (p < 0.001, p = 0.019). In the 35 patients with limb preservation, the median International Society of Limb Salvage (ISOLS) score at the final follow-up was 80 (47% to 97%). Younger age (p = 0.027) and complications (p = 0.005) were poor prognostic factors. A negative correlation was found between age and leg-length discrepancy (R = −0.426; p = 0.011). The ISOLS scores were significantly lower in patients with a leg-length discrepancy of more than 5 cm (p = 0.005).ConclusionYoung age and complications were linked to an unfavourable functional prognosis. Leg-length correction was insufficient, especially in very young children, resulting in decreased function of the affected limb. Limb-sparing surgery for these children remains a considerable challenge.Cite this article: Bone Jt Open 2024;5(10):868–878.
Cognitive learning enabled agile optical network
Authors: Cheng Yijun , Chen Zejun , Hu Zihe , Xiang Meng , Yan Zhijun , Qin Yuwen , Fu Songnian 
Published: October 2024

Nonlinear equalization (NLE) is essential for guaranteeing the performance of an optical network (ON). Effective NLE implementation relies on key parameters of the transmission link, including the modulation format (MF) and the launch power. As ONs become more agile, the parameters of fiber optical transmission need to be adaptive and relevant to the routing condition. Therefore, successful NLE implementation relies on the realization of transmission awareness (TA). Although machine learning-enabled optical performance monitoring (OPM) has been extensively investigated in the past few years, current NLE algorithms cannot autonomously perceive transmission parameters. Furthermore, current TA implementation still needs human intervention to guide the NLE. In addition, existing ML-based OPM and NLE cannot be trained autonomously, leading to the incapability of environmental change and mislabeling. Here, we propose cognitive learning (CL) for TA-guided NLE in agile ONs. We perform an experiment involving 32 Gbaud polarization-division-multiplexed (PDM)-quadrature phase shift keying (QPSK)/16-quadrature amplitude modulation (QAM) transmission over 1500 km of standard single-mode fiber (SSMF) with a variable launch power from 0 to 3 dBm. When a deep neural network (DNN) with amplitude histograms (AHs) as inputs and one step per span-learned digital back-propagation (1stps-LDBP) are developed, the CL simultaneously enables both TA and NLE, with the capability of self-learning, mislabeling resistance, and dynamic adaptation. The proof-of-concept experimental results indicate that both the accuracy of TA and the Q-factor of PDM-16QAM can be improved by 34.8% and 0.84 dB, respectively, when the launch power is 3 dBm. Moreover, the accuracy of TA is enhanced by 35.3%, even when the used data has 30% mislabeling. Therefore, the CL framework can be customized to satisfy various NLE implementations, thereby supporting the adaptive transmission of agile ONs.
Tunable reflective filters and perfect mirrors using all-dielectric metasurfaces
Authors: Singhal Sridhar , Sharma Shubhanshi , Varshney Shailendra K. 
Published: October 2024

For several years, extensive research has focused on narrow and broadband optical filters and mirrors. The aim has been to achieve highly specific spectral responses across different wavelength bands. All-dielectric materials have been preferred due to their low losses and high-quality factor values. In this study, we present meta-resonators with concave and convex shapes to achieve both narrow and broad spectral responses. Numerical simulations based on the finite element method reveal a full width at half-maximum (FWHM) of 0.65 nm and 346 nm. The concave and convex designs improve light-matter interaction and enable tunability. Using an appropriate thermo-responsive or electro-responsive material as a covering layer, we successfully achieved precise tunability of 0.3 nm/10°C and 0.18 nm/V within the concave metasurface. These metasurfaces demonstrate nearly perfect reflectance, allowing them to behave as perfect mirrors for narrow and broadband line width applications.
Reduction in the incidence of cognitive impairment and related costs through an innovative health awareness programme in rural Japan
Authors: Shoji Ayako , Kudo Kenichi , Murashita Koichi , Nakaji Shigeyuki , Igarashi Ataru 
Published: October 2024

Objectives
This study examined the impact of the Center of Healthy Aging Program (CHAP) on the cognitive function and economic burden associated with dementia.

Methods
This observational study utilised Iwaki cohort data. We included participants with mini-mental state examination (MMSE) scores and categorised them into pre- and post-CHAP groups based on their year of entry into the cohort (before 2013 or after) (index year). We defined participants with suspected severe cognitive impairment and suspected mild cognitive impairment using their MMSE scores, with their incidence being the first observation meeting these definitions during the follow-up period. We compared the incidence rates between the pre- and post-CHAP groups using Cox proportional hazard analysis. Medical and caregiving costs were estimated based on the projected number of residents in Iwaki area with suspected mild cognitive impairment and sSCI during hypothetical 10 years of the CHAP implemented or not and compared.

Results
Of the 2,569 participants, 1716 and 853 were included in the pre- and post-CHAP groups, respectively. The incidence rate of suspected mild cognitive impairment was significantly lower in the post-CHAP group even after adjusted known factors associated with cognitive disorders. No cases of suspected severe cognitive impairment occurred in the post-CHAP group during the follow-up period. Estimated costs of JPY 1,628,450 (USD 11562.00 or EUR 10259.24, JPY 100  =  USD 0.71 or EUR 0.63) and JPY 789,560 (USD 5605.88 or EUR 4974.23) per person per year were projected after 10 years with and without the CHAP, respectively.

Conclusions
We demonstrated a reduction in the incidence rate of suspected mild cognitive impairment among residents who participated in the CHAP and a decrease in the medical and caregiving costs associated with suspected severe cognitive impairment.
Delivering a pilot Institution Wide Language Programme (IWLP) at an Irish University: teachers’ perspectives
Authors: Bruen Jennifer , López Cuenca Elena 
Published: October 2024

Abstract
This paper analyses the language educator’s experience of delivering an Institution-Wide Language Programme (IWLP) in an Irish University. It uses a focus group to explore the experiences of six International Language Tutors who delivered modules on a pilot IWLP between 2021 and 2024. The findings indicate that designing and delivering the programme was a predominantly rewarding experience for the tutors involved. Positive aspects included engagement levels among students who completed the programme, the freedom to design, deliver and assess modules in a flexible and innovative manner, and the supportive nature of the tutor team. Negative aspects relate to policy issues concerning student eligibility for the programme, operational issues such as (de-)registration, attendance and attrition rates, and misalignment between student and module proficiency levels. Recommendations relate to IWLP design and administration as well as to supports for IWLP language educators. They include the use of hybrid teaching as standard, the opening of IWLPs to all staff and students (using caps on numbers and waiting lists), clear communication to potential participants in relation to the relationship between IWLP language modules and main degrees reflected in simplified registration, deregistration and certification processes, the provision of training in hybrid design and delivery of language modules as well as of appropriate teaching infrastructure, and full integration of the IWLP teaching team into institutional support structures.
Structural and anatomical analysis of rattan (Calamus balansaeanus Becc.)
Authors: Yu Linpeng , Zhang Kangjian , Dai Fukuan , Chen Yuxuan , Jiang Zehui , Tian Genlin , Wang Youhong 
Published: October 2024

Abstract

Calamus balasaeanus Becc., a climbing palm species, has potential commercial value. Detailed anatomical and chemical analyses of rattan stems at different ages are crucial for their utilization in various industrial applications, particularly eco-friendly products. In this study, rattan stems of different ages were examined using light microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The results showed that the anatomical structure of rattan stems at different ages are very similar, with vascular bundle sizes ranging from 0.26 to 0.37 mm and a frequency of 8.4 to 13.7 bundles/mm2. The vessel lengths and diameters were 1.87–2.35 mm and 0.10–0.16 mm, respectively. Fiber lengths and diameters were 0.84–1.15 mm and 8.65–11.55 μm, respectively. As C. balasaeanus matured, the secondary wall of fiber cells thickened into the cell lumen, changing from 2 layers to 4 layers, the amount of crystallinity in cellulose increased while the mean microfibril angle decreased. The crystallinity of cellulose was higher in the periphery of the stem compared to the center. Unimodal imaging effectively visualized the distribution of various polysaccharides in C. balasaeanus tissues, with the highest concentrations of cellulose, hemicellulose, and lignin found in the base of the rattan stems.
How We Communicate Forgiveness to Our Partners Matters: Forgiveness-Granting Strategies, Forgiveness Tendency, Commitment, and Relationship Satisfaction in Dating and Married Couples
Authors: Dršťáková Žofia , Záhorcová Lucia , Teličák Peter 
Published: October 2024

Abstract
Individuals in dating and marital relationships experience various transgressions that require forgiveness (e.g., hurtful communication, mistrust, lies, or infidelity). This study examines forgiveness-granting strategies in the context of forgiveness tendency, relationship length, commitment, satisfaction, and relational consequences to better understand relationship dynamics. 649 individuals (532 dating, 117 married), aged 18–65 (M = 26.00, SD = 8.82) participated. The sample included 517 women (79.7 %) and 132 men (20.3 %). Participants described a forgiven transgression and completed questionnaires, including the Multidimensional Forgiveness-Granting Scale (MFGS), Transgression-Related Interpersonal Motivations Inventory (TRIM-18), Relationship Assessment Scale (RAS), and the commitment subscale from the Investment Model Scale. One open-ended question (types of forgiven transgressions) was analyzed consensually. Quantitative data were analyzed using correlational analysis, regression analysis, and t-tests. The most common transgression was infidelity. A linear regression analysis showed that the strongest positive predictor of relationship satisfaction was the tendency to forgive one’s partner. Nonverbal displays positively predicted relationship strengthening, while the discussion strategy predicted relationship deterioration negatively. These findings emphasize the importance of how forgiveness is communicated and may inform interventions to enhance romantic relationships.
The Minimum Wage in Greece: A Review of Institutional Features, Developments and Effects Between 1975 and 2023
Authors: Nicolitsas Daphne 
Published: October 2024

Abstract
This paper takes a historical perspective and assesses the evolution of the institutional features in setting the minimum wage in Greece between 1975 and 2023. It also evaluates developments regarding the minimum wage level, its bite and alignment with productivity. The paper reviews the limited available empirical literature on the association of the minimum wage with labour market outcomes (average wages, employment, inequality). It presents new estimates of the elasticity of average wages to the minimum wage. One of the paper’s key points is that the minimum wage setting mechanism has changed over time as the economic environment has changed. Reviewing the evolution of the minimum wage over time to evaluate whether the minimum wage follows productivity developments and whether the minimum wage bites leads to the second and third takeaways of the paper. The minimum wage follows productivity developments over the longer term but not always in the short term. The bite of the minimum wage is high and appears to be higher when government intervention in setting the minimum wage is stronger. As for the impact of the minimum wage on average wages, the new estimates of the elasticity of the average to the minimum wage the paper provides, use more precisely measured wage rates, which show a high elasticity of average to minimum wages. Finally, the review of the existing literature on the employment effects of the minimum wage shows that, as in other countries, the results are mixed with modest negative or no effects.