bluuebunny/crossref_metadata_2025_split huggingface dataset:
Additive effects of mean temperature, temperature variability, and chlorothalonil to red-eyed treefrog (Agalychnis callidryas) larvae
Authors: Alza Carissa M. , Donnelly Maureen A. , Whitfield Steven M. 
Published: May 2016

Abstract

Amphibian populations are declining globally, and multiple anthropogenic stressors, including contamination by pesticides and shifting climates, are driving these declines. Climate change may increase average temperatures or increase temperature variability, either of which may affect the susceptibility of nontarget organisms to contaminants. Eight-day ecotoxicological assays were conducted with red-eyed treefrog (Agalychnis callidryas) larvae to test for additive and interactive effects of exposure to the fungicide chlorothalonil, average temperature, and temperature variability on tadpole growth and survival. Egg masses were collected from seasonal ponds at La Selva Biological Station in Costa Rica, and tadpoles were exposed to a series of chlorothalonil concentrations across a range of ecologically relevant mean temperatures (23.4–27.3 °C) and daily temperature fluctuations (1.1–9.9 °C). Survival was measured each day, and tadpole growth was measured at the end of each trial. Concentrations of chlorothalonil ≥60 µg/L reduced survival, although survival was not affected by mean temperature or daily temperature range, and there were no synergistic interactions between chlorothalonil and temperature regime on survival. Chlorothalonil suppressed tadpole growth at relatively low concentrations (∼15 µg/L). There were impacts of both average temperature and daily temperature range on tadpole growth, although there were no synergistic interactions between temperature regimes and chlorothalonil. The results should inform efforts to manage ecosystems impacted by multiple large-scale anthropogenic stressors as well as methods for the design of ecologically appropriate toxicology trials. Environ Toxicol Chem 2016;35:2998–3004. © 2016 SETAC
Association of WT1 IgG antibody against WT1 peptide with prolonged survival in glioblastoma multiforme patients vaccinated with WT1 peptide
Authors: Oji Yusuke , Hashimoto Naoya , Tsuboi Akihiro , Murakami Yui , Iwai Miki , Kagawa Naoki , Chiba Yasuyoshi , Izumoto Shuichi , Elisseeva Olga , Ichinohasama Ryo , Sakamoto Junichi , Morita Satoshi , Nakajima Hiroko , Takashima Satoshi , Nakae Yoshiki , Nakata Jun , Kawakami Manabu , Nishida Sumiyuki , Hosen Naoki , Fujiki Fumihiro , Morimoto Soyoko , Adachi Mayuko , Iwamoto Masahiro , Oka Yoshihiro , Yoshimine Toshiki , Sugiyama Haruo 
Published: May 2016

We previously evaluated Wilms’ tumor gene 1 (WT1) peptide vaccination in a large number of patients with leukemia or solid tumors and have reported that HLA‐A*24:02 restricted, 9‐mer WT1‐235 peptide (CYTWNQMNL) vaccine induces cellular immune responses and elicits WT1‐235‐specific cytotoxic T lymphocytes (CTLs). However, whether this vaccine induces humoral immune responses to produce WT1 antibody remains unknown. Thus, we measured IgG antibody levels against the WT1‐235 peptide (WT1‐235 IgG antibody) in patients with glioblastoma multiforme (GBM) receiving the WT1 peptide vaccine. The WT1‐235 IgG antibody, which was undetectable before vaccination, became detectable in 30 (50.8%) of a total of 59 patients during 3 months of WT1 peptide vaccination. The dominant WT1‐235 IgG antibody subclass was Th1‐type, IgG1 and IgG3. WT1‐235 IgG antibody production was significantly and positively correlated with both progression‐free survival (PFS) and overall survival (OS). Importantly, the combination of WT1‐235 IgG antibody production and positive delayed type‐hypersensitivity (DTH) to the WT1‐235 peptide was a better prognostic marker for long‐term OS than either parameter alone. These results suggested that WT1‐235 peptide vaccination induces not only WT1‐235‐specific CTLs as previously described but also WT1‐235‐specific humoral immune responses associated with antitumor cellular immune response. Our results indicate that the WT1 IgG antibody against the WT1 peptide may be a useful predictive marker, with better predictive performance in combination with DTH to WT1 peptide, and provide a new insight into the antitumor immune response induction in WT1 peptide vaccine‐treated patients.
Non‐clinical safety assessment of single and repeated administration of gE/AS01 zoster vaccine in rabbits
Authors: Giordano Giulia , Segal Lawrence , Prinsen Menk , Wijnands Marcel V. W. , Garçon Nathalie , Destexhe Eric 
Published: May 2016

AbstractHZ/su is an investigational recombinant subunit vaccine for the prevention of shingles, a disease resulting from the reactivation of varicella zoster virus. The vaccine is composed of recombinant varicella zoster virus glycoprotein E (gE), and liposome‐based Adjuvant System AS01. To evaluate the potential local and systemic effects of this vaccine, three studies were performed in rabbits. In the first two studies, rabbits received a single intramuscular (IM; study 1) or subcutaneous (SC; study 2) dose of gE/AS01, AS01 alone (in study 2 only) or saline, and the local tolerance was evaluated up to 3 days after administration. Under these conditions, only local inflammatory reactions at the injection sites were detected by microscopic evaluation. In the third study, gE/AS01, AS01 alone or saline, were injected SC or IM on four occasions at 2 week intervals. General health status, local tolerance, ophthalmology, haematology and blood chemistry parameters were monitored. Macroscopic and microscopic evaluations were performed after termination of the study. The only treatment‐related changes included a transient increase in neutrophils, C‐reactive protein and fibrinogen levels and microscopic signs of inflammation at the injection sites, which are expected observations related to the elicited inflammatory reaction. The SC and IM routes of administration produced similar systemic effects. However, microscopic findings at the injection sites differed. One month after the last injection, recovery was complete in all groups. In conclusion, the single and repeated SC and IM administration of the gE/AS01 vaccine were locally and systemically well‐tolerated in rabbits and support the clinical development of the vaccine. Copyright © 2016 John Wiley & Sons, Ltd.
The representation of health‐relevant heatwave characteristics in a Regional Climate Model ensemble for New South Wales and the Australian Capital Territory, Australia
Authors: Gross Mia H. , Alexander Lisa V. , Macadam Ian , Green Donna , Evans Jason P. 
Published: May 2016

ABSTRACTHeatwaves have been linked to increased rates of human mortality and morbidity. Given these adverse health impacts, it is crucial to improve our understanding of future changes in these extreme events to inform health impacts studies and adaptation planning. While this information would be most beneficial at a local scale, Global Climate Models provide projections on much coarser resolutions. Regional Climate Models, such as those used in the New South Wales/Australian Capital Territory Regional Climate Modelling (NARCliM) project, provide simulations at a finer scale more appropriate for regional assessments. This study uses NARCliM output to investigate the ability of a Regional Climate Model ensemble to represent heatwave characteristics through the Excess Heat Factor, an index that includes factors that are known to be important to the heat‐health relationship. Both uncorrected and bias‐corrected model output is evaluated against observationally‐derived heatwave characteristics for the period 1990–2009. The effect of bias‐correction on future changes in heatwave characteristics is also assessed. Overall, the simulations provided a good representation of the recent climate and bias‐correction did not greatly change simulated heatwave characteristics. Some regions were more affected by bias‐correction than others, with bias‐correction being most beneficial for coastal regions. We emphasise that these results may not apply to all indices measuring extreme heat, and demonstrate that results for an index based on a fixed absolute temperature threshold are substantially affected when bias‐correction is applied. While supporting bias‐correction, this study demonstrates that it is not necessarily required when evaluating a relative measure such as the Excess Heat Factor.
Food Design Thinking: A Branch of Design Thinking Specific to Food Design
Authors: Zampollo Francesca , Peacock Matthew 
Published: May 2016

AbstractIs there a need for a set of methods within Design Thinking tailored specifically for the Food Design process? Is there a need for a branch of Design Thinking dedicated to Food Design alone? Chefs are not generally trained in Design or Design Thinking, and we are only just beginning to understand how they ideate and what recourses are available to them. Given the sheer variety of eating situations, and the complexity of the factors influencing them, a design method that specifically stimulates thinking about Food Design would be very useful. I introduce TED (Themes for Eating Design, a design method developed to generate themes on the ideal eating situation, which summarizes the themes it has generated in previous research, and how these themes have been transformed into a tool for the idea generation phase called “Thoughts For Food”). I present an initial test of TED and Thoughts For Food in practice, with groups using them to design an eating experience. Initial results suggest both have promise for designing food or any other aspect of the eating experience and that these methods can form part of a new branch of design theory that I call Food Design Thinking.
Rotator cuff repair using cell sheets derived from human rotator cuff in a rat model
Authors: Harada Yoshifumi , Mifune Yutaka , Inui Atsuyuki , Sakata Ryosuke , Muto Tomoyuki , Takase Fumiaki , Ueda Yasuhiro , Kataoka Takeshi , Kokubu Takeshi , Kuroda Ryosuke , Kurosaka Masahiro 
Published: May 2016

ABSTRACTTo achieve biological regeneration of tendon‐bone junctions, cell sheets of human rotator‐cuff derived cells were used in a rat rotator cuff injury model. Human rotator‐cuff derived cells were isolated, and cell sheets were made using temperature‐responsive culture plates. Infraspinatus tendons in immunodeficient rats were resected bilaterally at the enthesis. In right shoulders, infraspinatus tendons were repaired by the transosseous method and covered with the cell sheet (sheet group), whereas the left infraspinatus tendons were repaired in the same way without the cell sheet (control group). Histological examinations (safranin‐O and fast green staining, isolectin B4, type II collagen, and human‐specific CD31) and mRNA expression (vascular endothelial growth factor; VEGF, type II collagen; Col2, and tenomodulin; TeM) were analyzed 4 weeks after surgery. Biomechanical tests were performed at 8 weeks. In the sheet group, proteoglycan at the enthesis with more type II collagen and isolectin B4 positive cells were seen compared with in the control group. Human specific CD31‐positive cells were detected only in the sheet group. VEGF and Col2 gene expressions were higher and TeM gene expression was lower in the sheet group than in the control group. In mechanical testing, the sheet group showed a significantly higher ultimate failure load than the control group at 8 weeks. Our results indicated that the rotator‐cuff derived cell sheet could promote cartilage regeneration and angiogenesis at the enthesis, with superior mechanical strength compared with the control. Treatment for rotator cuff injury using cell sheets could be a promising strategy for enthesis of tendon tissue engineering. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:289–296, 2017.
Stabilization of red fruit‐based smoothies by high‐pressure processing. Part A. Effects on microbial growth, enzyme activity, antioxidant capacity and physical stability
Authors: Hurtado Adriana , Guàrdia Maria Dolors , Picouet Pierre , Jofré Anna , Ros José María , Bañón Sancho 
Published: May 2016

AbstractBACKGROUNDNon‐thermal pasteurization by high‐pressure processing (HPP) is increasingly replacing thermal processing (TP) to maintain the properties of fresh fruit products. However, most of the research on HPP‐fruit products only partially addresses fruit–pressure interaction, which limits its practical interest. The objective of this study was to assess the use of a mild HPP treatment to stabilize red fruit‐based smoothies (microbial, enzymatic, oxidative and physical stability).RESULTSHPP (350 MPa/10 °C/5 min) was slightly less effective than TP (85 °C/7 min) in inactivating microbes (mesophilic and psychrophilic bacteria, coliforms, yeasts and moulds) in smoothies kept at 4 °C for up to 28 days. The main limitation of using HPP was its low efficacy in inactivating oxidative (polyphenol oxidase and peroxidase) and hydrolytic (pectin methyl esterase) enzymes. Data on antioxidant status, colour parameters, browning index, transmittance, turbidity and viscosity confirmed that the HPP‐smoothies have a greater tendency towards oxidation and clarification, which might lead to undesirable sensory and nutritional changes (see Part B).CONCLUSIONThe microbial quality of smoothies was adequately controlled by mild HPP treatment without affecting their physical‐chemical characteristics; however, oxidative and hydrolytic enzymes are highly pressure‐resistant, which suggests that additional strategies should be used to stabilize smoothies. © 2016 Society of Chemical Industry
Outcomes and predictors of surgical management in type 1 laryngeal cleft swallowing dysfunction
Authors: Thottam Prasad John , Georg Matthew , Chi David , Mehta Deepak K. 
Published: May 2016

Objectives/HypothesisTo examine the effect of and predict the success of type 1 laryngeal cleft (LC‐1) augmentation through swallowing evaluations.Study DesignRetrospective chart analysis.MethodsSixty‐eight patients with LC‐1s underwent interarytenoid injection laryngoplasty (IL) and were examined. The median age at IL was 9 months. Swallowing evaluations were performed pre‐ and postoperatively using fiberoptic endoscopic examination of swallowing or modified barium swallow. The presence of aspiration or penetrations at various consistencies was recorded. McNemar's tests were used to detect changes in swallowing pre‐ and postoperatively. Logistic regression was used to assess factors affecting the odds of postoperative success.ResultsPreoperatively, 89.7% of patients demonstrated penetration or aspiration. Post‐IL, 69.1% were safe for thins, and 75% showed improvement in swallowing. Postoperatively, there was a significant reduction in patients experiencing problems with thin liquids (P < 0.001) and in those with frank or silent aspiration (P < 0.001). Patients with penetrations on thin liquids had higher likelihood of a successful IL (odds ratio [OR] = 3.68, P = 0.021). The probability of success with silent aspiration at any consistency was significantly decreased (OR = 0.26, P = 0.015). Fifteen patients underwent formal endoscopic surgical repair, and 90.0% were safe with thin consistencies postoperatively.ConclusionA large proportion of patients with LC‐1 and associated swallowing dysfunctions respond favorably to IL and formal repair. Children who demonstrated penetration with thin liquids had a higher rate of swallowing dysfunction resolution post‐IL; whereas patients demonstrating silent aspiration had poorer responses to IL.Level of Evidence4. Laryngoscope, 126:2838–2843, 2016
Removal of methane through hydrological, microbial, and geochemical processes in the shallow sediments of pockmarks along eastern Vestnesa Ridge (Svalbard)
Authors: Hong Wei‐Li , Sauer Simone , Panieri Giuliana , Ambrose William G. , James Rachael H. , Plaza‐Faverola Andreia , Schneider Andrea 
Published: May 2016

AbstractThe recent discovery of methane seeps in the Arctic region requires a better understanding of the fate of methane in marine sediments if we are to understand the contributions of methane to Arctic ecosystems and climate change. To this goal, we analyze pore water data from five sites along eastern Vestnesa Ridge, a sediment drift off‐north‐west Svalbard, to quantify the consumption of dissolved methane across the sulfate‐methane‐transition‐zone which are 3–5 m below seafloor from the investigated sites. We use transport‐reaction models to quantify the hydrology as well as the carbon mass balance in the sediments. Pore water profiles and our model results demonstrate that hydrological, microbial, and geochemical processes/reactions efficiently remove methane carbon from fluid over different time scales. We interpret the nonsteady‐state behavior of the first 50–70 cm of our pore water profiles from the active sites as an annual scale downward fluid flow due to a seepage‐related pressure imbalance. Such downward flow supplies sulfate which enhances methane consumption through anaerobic oxidation of methane (AOM) within this depth range. Our steady‐state modeling confirms the efficiency of AOM in consuming dissolved methane in the upper 0.8–1.2 m of sediments. Based on the phosphate profiles, we estimate that AOM at the active pockmarks may have been operating for the last two to four centuries. Precipitation of authigenic carbonate removes more than a quarter of the dissolved inorganic carbon produced by AOM and fixes it as authigenic carbonate in the sediments, a process that sequestrates methane carbon over geological time.
Relational Domain Switching: Interpersonal Insecurity Predicts the Strength and Number of Marketplace Relationships
Authors: Whelan Jodie , Johnson Allison R. , Marshall Tara C. , Thomson Matthew 
Published: May 2016

ABSTRACTIn this paper, the authors test a compensation model of interpersonal and marketplace relationships. Guided by an attachment theory perspective, the authors argue that reflecting on or experiencing insecure interpersonal relationships can induce consumers to seek surrogate relationship partners in the marketplace. This general prediction is supported by results from an experiment and two surveys. Specifically, results show that interpersonally anxious consumers report more and stronger brand relationships. Furthermore, interpersonally avoidant consumers report more and stronger brand relationships, as well as more numerous but weaker service relationships. These studies support the prediction that people employ marketplace solutions to offset deficiencies in their personal relationships. The paper concludes by contextualizing the results within research on loneliness and materialism.
SEM and TEM for structure and properties characterization of bacterial cellulose/hydroxyapatite composites
Authors: Arkharova Natalia A. , Suvorova Elena I. , Severin Alexander V. , Khripunov Albert K. , Krasheninnikov Sergey V. , Klechkovskaya Vera V. 
Published: May 2016

SummaryPreparation of composites with different properties and gradient of components is aimed at better performance of materials for bone substitution. Bacterial cellulose‐hydroxyapatite (BC‐HAP) composites with various mass ratio of the components (BC‐25HAP, BC‐4HAP, and BC‐HAP) were prepared by a novel method of growing HAP nanocrystals (the linear size ≤30 nm) in water solutions in the presence of the BC gel‐film micro‐fragments. Varying the BC‐HAP ratios leads to a gradual change of the physical properties of the materials. It was found that an increase in the BC content results in a decrease of the HAP crystal length and specific surface area, porosity, and pore volume while the values of density and Young's modulus values increase. SCANNING 38:757–765, 2016. © 2016 Wiley Periodicals, Inc.
A universal method for realizing non‐repudiable provable data possession in cloud storage
Authors: Wang Hongyuan , Zhu Liehuang , Xu Chang , Lilong Yijia 
Published: May 2016

AbstractProvable data possession (PDP) and proofs of retrievability (POR) are techniques for a client to verify the integrity of outsourced data on an untrusted server, and numerous PDP/POR schemes with public or private verification have been proposed so far. However, existing schemes cannot handle the following issue satisfactorily: Driven by profits, a malicious client may accuse an honest server and repudiate the correct proof of data possession. Based on the commitment function, we present a universal method that can reform any private verification PDP/POR scheme into a non‐repudiable PDP (NRPDP)/POR scheme. As a case study, we propose a concrete NRPDP scheme with private verification, which allows both the client and the server to prove their innocence and verify whether the other side is honest. Moreover, we prove the security of both the method and NRPDP in the random oracle model and implement a prototype based on the NRPDP scheme in a realistic cloud platform. Experimental results on large dataset (10GB) show that the NRPDP can be executed efficiently as private verification schemes and outperforms the public schemes more than 60% in terms of verification time. Foremost, non‐repudiation can be guaranteed efficaciously in NRPDP. Copyright © 2016 John Wiley & Sons, Ltd.
Event-based run-time adaptation in communication-centric systems
Authors: Di Giusto Cinzia , Pérez Jorge A. 
Published: May 2016

Abstract

Communication-centric systems
are software systems built as assemblies of distributed artifacts that interact following predefined communication protocols.
Session-based concurrency
is a type-based approach to ensure the conformance of communication-centric systems to such protocols. This paper presents a model of
session-based concurrency
with mechanisms for
run-time adaptation
. Our model allows us to specify communication-centric systems whose session behavior can be dynamically updated at run-time. We improve on previous work by proposing an
event-based
approach: adaptation requests, issued by the system itself or by its context, are assimilated to events which may trigger adaptation routines. These routines exploit type-directed checks to enable the reconfiguration of processes with active protocols. We equip our model with a type system that ensures
communication safety
and
consistency
properties: while safety guarantees absence of run-time communication errors, consistency ensures that update actions do not disrupt already established session protocols. We provide soundness results for binary and multiparty protocols.