bluuebunny/crossref_metadata_2025_split huggingface dataset:
JAK/STAT signaling pathway inhibitors for leukemia therapeutic potential : a systematic review on in vitro literatures
Authors: Karimi Zahed , Shahvari Samira Zakeri , Ghasemi Katayoon , Faridpour Amir Abbas , Baradaran Mohammad Amin , Satarzadeh Mohammad 
Published: September 2024

Abstract

Background Janus kinases (JAKs), signal transducer and activator of transcription proteins (STATs) is a main signaling pathway in the immune system, autoimmune disease and in many malignancies such as leukemia. Limiting this signaling pathway has so far been investigated in the treatment of some diseases such as lymphoma, and in this systematic review, we will investigate the role of this type in leukemia. Method This systematic review was written based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria and the principle of non-bias was respected. All the articles from 2014–2024 were extracted from Web of Science, PubMed and Scopus databases. We extracted the extracting inhibitors, related immunity elements, JAK/STAT kind, side signaling pathway. Results Three authors searched the database, after screening full texts, 17 articles were included. 15 inhibitory drugs for the JAK/STAT pathway were extracted in studies, which inhibit this signaling pathway in vitro and in cultured human leukemic cells. The most signaling pathways in leukemia were limited to STAT 5 and STAT 3, which were inhibited by Thymoquinone, Ritanserin, Dactocilib and Ruxolitinib. On the other hand, leukemic cells act exclusively through the JAK 2 pathway, which was specifically inhibited by Ruxolitinib and AG490. Increased apoptosis of leukemic cells and decreased cell survival was the result of monotherapy and multitherapy of these inhibitors in the studies available to us. Conclusion Inhibition of JAK/STAT pathway is associated with the reduction of leukemic cell growth and apoptosis of these types of cells, which seems to be a great therapeutic potential for clinical studies in all types of leukemia. We suggest that future studies investigate this type of treatment especially in children and evaluate our results.
N-glycosylation of GSTO1 promotes cervical cancer migration and invasion through JAK/STAT3 pathway activation
Authors: Yu Panpan , Zhao Zouyu , Sun Qianyu , Diao Bowen , Sun Chongfeng , Wang Yan , Qiao Hui , Li Hong , Yang Ping 
Published: September 2024

Abstract

Background Protein glycosylation is strongly associated with tumor progression. Glutathione S-transferase omega 1 (GSTO1) is a member of the glutathione S-transferase family. The significance of GSTO1 N-glycosylation in the progression of cervical cancer (CC) has remained elusive. In this study, we investigated the functional significance of GSTO1 N-glycosylation in CC progression. Methods We employed immunohistochemistry to detect the relative expression of evaluating the link between GSTO1 in CC and benign tissues and the overall survival (OS) and progression-free survival (PFS) in CC patients. In vitro and in vivo experiments to detect CC cell proliferation or metastatic ability after GSTO1 downregulation. NetNGly1.0 Server database predicts potential N-glycosylation modification sites of GSTO1 (Asn55, Asn135, Asn190). Investigating GSTO1 N-glycosylation's function in cellular migration, invasion and epithelial–mesenchymal transition (EMT), we mutated the N-glycosylation sites of GSTO1 through lentivirus-based insertional mutagenesis. Detection of signalling pathways associated with N-glycosylation-modified GSTO1 by enrichment analysis and Western blot. Results Compared to normal cervical tissue, CC tissue showed significantly higher GSTO1 expression. Further, high GSTO1 levels were a poor predictor of OS and PFS. Both cell and animal experiments suggested that down-regulation of GSTO1 inhibited cell proliferation and metastasis. Glycosylation modification of targeted mutant GSTO1 at positions 55, 135 and 190 significantly inhibits migration and invasion of CC cells. GSTO1 N-glycosylation fixed point mutation inhibits EMT process in CC cells. Mechanistically, N-glycosylated GSTO1 promoted the expression of JAK/STAT3 pathway related markers. Conclusions GSTO1 N-glycosylation is associated with CC progression and may promote EMT via JAK/STAT3 signaling.
Clinical and molecular investigation of Acute Haemorrhagic Conjunctivitis outbreak in North India
Authors: Thakur Anchal , Sharma Deepanjli , Singh Mini P , Chauhan Poonam , Shah Aryan , Angra Vani , Ghosh Arnab , Chintan Malhotra , Goyal Kapil , Gupta Amit 
Published: September 2024

Abstract

Purpose
The study aims to investigate an outbreak of acute haemorrhagic conjunctivitis (AHC) reported in North India.
Methods
All patients presenting to the outpatient clinic department with signs and symptoms (symptoms onset within 36 hours) of AHC were enrolled in the study. The conjunctival swab samples were tested for the presence of enteroviruses and adenoviruses. Detailed clinical features of all positive patients were noted. Representative enterovirus-positive samples were sequenced for viral; protein 1 (VP1) gene for type identification. The sequences were analysed by the Basic Local Alignment Search Tool (BLAST) software and enterovirus genotyping tool v1.0. A maximum likelihood tree was constructed using MEGA11.
Results
Out of 133 samples tested, 75.1% (n = 100) samples were positive for enterovirus by real-time PCR. The average duration of onset was 16.9 hours. Subconjunctival haemorrhage was observed in 67% of the patients, while 30% of cases had conjunctival chemosis. The causative agent of the current outbreak was identified as Coxsackievirus A24v G-IV. A sequence homology of 96–99% and pairwise distance divergence of 2.9% was observed between study sequences and recent sequences of China outbreak. Mutation analysis showed four common mutations (T281C, A311G, T332C, and T395C) with those of Chinese sequences, however no new mutations were identified in current study sequences, suggesting a close relatedness to CVA24 sequences from China.
Conclusions
Coxsackievirus A24v continues to be an important agent of Acute Haemorrhagic conjunctivitis in North India. In view of its highly contagious nature, effective and timely public health measures are required to limit its spread.
Prognostic Role Of Naples Prognostic Score In Lung Cancer: A Meta-Analysis
Authors: Han Zhaohui , Wang Zengming , Zhao Chunyan , Zhang Xiaopeng , Niu Zhancong , Zhao Qingtao , Duan Guochen 
Published: September 2024

Abstract

Purpose:The prognostic value of the Naples prognostic score in lung cancer remains controversial. Therefore, we performed a meta-analysis of relevant published studies to determine the prognostic value of the Naples prognostic score in patients with lung cancer.Methods:We conducted a systematic search of relevant studies in PubMed, Ovid, the Cochrane Library, and Web of Science databases. Data and characteristics of each study were extracted and hazard ratios (HRs) at 95% confidence intervals (CIs) were calculated to estimate effects. A meta-regression analysis was used to assess the prognostic value of the Naples Prognostic Score in patients with lung cancer.Results:A total of 1691 patients from six studies were included in this meta-analysis, with a combined HR of 3.357 (95% CI: 1.964-5.738, p=0.000); the results suggest that a high Naples Prognostic Score predicts a shorter overall survival (OS) for patients.Conclusion:This meta-analysis suggests that a high Naples Prognostic Score may be a predictor of poor prognosis in lung cancer patients. Further large cohort studies are needed to confirm these findings.
Microalgae as Feed: Growth, Improvement and Filtration Efficiency of Green Mussel (Perna viridis) spat
Authors: Islam Zahidul , Hasan Jakia , Rahman Turabur , Aktaruzzaman Md. , Rahman Shafiqur , Tanu Mohosena Begum 
Published: September 2024

Abstract

Green mussel aquaculture is increasing day by day. This culture is mostly dependent on the use of natural seed/spat while some use of spat from hatcheries is also reported. The use of hatchery spat is more convenient where spat rearing with suitable feed in in-situ environment ensure quality spat production with maximum survival. So this study was designed to determine the efficacy and suitability of indigenous microalgae (Nannochloropsis sp., Isochrysis sp. and Tetraselmis sp.) as feed for Perna viridis spat production in hatchery condition. Result shows the mean survival rate was highest in the Isochrysis sp. fed spat (71.33 ± 1.52%), although it didn’t differ significantly among dietary treatment. In case of filtration and ingestion rate, Tetraselmis sp. fed spat had the highest filtration rate of 0.997 ± 0.02 mL/spat/h where Nannochlorpsis sp. fed spat had the highest ingestion rate of 7.99 × 106 ± 1.16 cells/spat/h. In case of growth, spat fed with Isochrysis had higher shell weight (0.23 ± 0.014 g), length (9.53 ± 0.34 mm), width (4.73 ± 0.22 mm) and depth (2.08 ± 0.05 mm) where the Nannochloropsis sp. diet showed the lowest growth. However, the data were not significantly different among each other in case of shell width and depth. Similarly, spat fed with Isochrysis sp. had higher instantaneous relative growth rate (0.00541 ± 0.0007 µm/day) than other two species. The result of the study showed that, all the three selected microalga diets can significantly affect the performance of the spat of green mussel but indicated the enhanced growth with Isochrysis sp. fed larvae. The output of the study will provide a better understanding in case of appropriate food selection in case of green mussel spat to improve their production in captivity.
Should lymphadenectomy be recommended in radical surgery of intrahepatic cholangiocarcinoma patients? A retrospective study
Authors: Zhang Ruoyu , Yang Min , Chen Bo , Ye Feng , Huang Ning , Liu Mei , Wang Liming 
Published: September 2024

Abstract

Purpose Intrahepatic cholangiocarcinoma (ICC) is an extremely deadly cancer with high recurrence incidence, particularly in patients with lymph node metastasis (LNM). The necessity of lymphadenectomy including lymph node biology (LNB) and dissection (LND) during ICC radical surgery remains debate.Methods We retrospectively analyzed the patients diagnosed with ICC and underwent radical surgery at the Cancer Hospital of the Chinese Academy of Medical Sciences from 2012 to 2023.Results A total of 308 ICC patients were involved in this study. pLNM + group had poorer OS (P < 0.0001) and poorer DFS (P < 0.0001) compared with pLNM- group. Compared to the LN- group, LN + group exhibited worse OS (P = 0.038) and worse DFS (P = 0.003). After PSM and IPTW, compared with LN- group, LNB exhibited longer operation time (all P < 0.05) and longer hospitalization days (all P < 0.05) with no significant differences in complications, DFS, and OS. Compared with LN- group, LND group had no better DFS and OS, only more complications (all P < 0.05), longer operation time (all P < 0.001), higher risk of bleeding (all P < 0.05), transfusion (IPTW: P = 0.027) and longer hospitalization days (IPTW: P = 0.0006). Compared with LNB group, LND had longer operation time (P = 0.0227), higher risk of bleeding (P = 0.032) and transfusion (P = 0.019), and more postoperative complications (P = 0.02), with no difference in DFS and OS.Conclusion LND only achieves the effect of LNB while negatively affects postoperative recovery without survival benefit for ICC patients. LND is not recommended while standardizing LNB is recommended as a routine operation during the radical surgery of ICC patients.
Magnetic resonance imaging patterns of children with cerebral palsy: findings from hospital-based surveillance in Vietnam
Authors: Karim Tasneem , Mohammad Shekeeb S , Dossetor Rachael , Nguyen Thi Huong Giang , Trinh Quang Dung , Nguyen Thi Van Anh , Khuc Thi Hong Hanh , Nguyen Van Bang , Badawi Nadia , Khandaker Gulam , Elliott Elizabeth J 
Published: September 2024

Abstract

BACKGROUND: Previous studies on neuroimaging in cerebral palsy (CP) identified inconsistent radiologic findings. The Magnetic Resonance Imaging Classification system (MRICS) was developed by the Surveillance of Cerebral Palsy in Europe to standardize MRI reporting. MRICS aids in predicting future impairments and treatment but it remains largely unexplored in low-and middle-income countries (LMICs) often due to the lack of available MRI data. This study aims to document brain MRI patterns among Vietnamese children with CP.
METHODS: Active prospective ascertainment of children with CP using hospital-based surveillance in the National Children’s Hospital, Hanoi, Vietnam between June and November 2017. Data on risk factors and clinical characteristics were collected. Brain MRI reports were obtained from hospital records and classified using the MRICS.
RESULTS: MRI data were available for 264 children with CP (mean age 2.5±2.1 years). MRI showed pathogenic patterns in 76.4%. Nearly half (44.7%) of the children had predominant white matter injury, with predominant grey matter injury in 9.5%, and maldevelopment in 5.3%. MRI findings were associated with the type of CP and timing of causative injury including prematurity, birth asphyxia and infection.
CONCLUSION: MRI findings are consistent with the predominance of white matter injury described in cohorts of children with CP from high income countries. This study furthers understanding of MRI patterns and the related aetiologic factors among children with CP in Vietnam which will inform preventive strategies in similar settings.
Novel Hamy Mean Aggregation OperatorsBased on Advanced Operations for T-Spherical Fuzzy Group Decision-Making
Authors: Wang Haolun , Zhao Wei , Feng Liangqing , Mahmood Tahir , Kumar Kamal 
Published: September 2024

Abstract

Some advanced operation laws (AdOLs) are put forward with the T-spherical fuzzy (TSF) information. A series of novel Hamy mean aggregation operators are developed, specifically TSF advanced Hamy mean (TSFAdHM), TSF advanced dual Hamy mean (TSFAdDHM), TSF advanced weighted Hamy mean (TSFAdWHM) and TSF advanced weighted dual Hamy mean (TSFAdWDHM) operators. These aggregation operators integrate the strengths of Hamy mean and AdOLs in that they not only reveal correlations among multi-input variables but also eliminate counter-intuitive problems, as well as reflect decision-maker risk preferences. Several reasonable natures and peculiar types of these aggregation operators are investigated. Moreover, a novel group decision-making framework for the type of TSF aggregation operator is constructed. The tractability and usefulness of the decision-making model are examined by some numerical examples. The sensitivity and method comparison analyses are used to demonstrate that the proposed model can remedy the drawbacks of existing methods, and this article offers a very useful method for the complicated group decision-making issues.
Synthesis of new polymeric composite materials derived from 2,2- (1,6-dioxohexane-1,6-diyl) dihydrazinecarbothioamide
Authors: Hasheem Fatimah A. , Attiya Hanaa G. , Saeed Ruwaidah S. , Al-Heetimi Dhafir T.A. 
Published: September 2024

Abstract

In the present study, synthesis and characterization of new polymeric nanocomposites. First, the compound [C1] was formed from the reaction of one mole of dimethyl adipate with two moles of thiosemicarbazide in the ethanol absolute, then compound [C1] was reacted with sodium hydroxide to yield compounds [C2]. Compound [C3] was synthesized by reaction of hydrazine hydrate with compound [C2]. Compound [C4] was synthetic from Maleic anhydride and compound [C3]. Compound [C4] reacted with ammonium per sulfate as an initiator to produce polymer [C5], and then this polymer was blended with nano: ZnNPs, AgNPs, SiNPs, or IONPs by using a hotplate stirrer for 3 hours to produce nanocomposites [C6-C9]. The synthesized compounds were identified via spectral analysis techniques, including FTIR, 1H-NMR, and Field Emission Scanning Electron Microscope (FESEM). In addition, the thermal properties of TGA and DSC were studied. Molecular docking is studied, where operations are used to predict the binding status of compounds with the enzyme and to calculate the free energy (ΔG) of the compounds prepared. Finally, study biological activities screened via two types of bacteria (Bacillus cereus and Escherichia coli) and anti-cancer activity against HepG2 (human liver cancer cell line) and compare with the normal cell line WRL68 (normal liver cell line) of polymers and nanocomposites.
Acceptability and Uptake of COVID-19 Vaccines among Pregnant and Lactating Women seeking services at a Tertiary Public Hospital in Kampala, Uganda
Authors: Katana Elizabeth B. , Odongpiny Eva Agnes Laker , Simbwa Brenda N. , Nsubuga Edirisa J. , Namayanja Josephine , Muhindo Richard , Kiiza Daniel , Castelnuovo Barbara , Ario Alex R. , Waitt Catriona 
Published: September 2024

Abstract

Background
Globally, several vaccines, including COVID-19 vaccines, have been routinely recommended during pregnancy and lactation. However, data on COVID-19 vaccine acceptability and uptake among pregnant and lactating women are limited in Sub-Saharan Africa. While COVID-19 is no longer a public health emergency it is important to take stock of lessons learnt to prepare for future health threats including those that disproportionately affect pregnant/lactating women. We aimed to assess acceptability and uptake of COVID-19 vaccines among pregnant and lactating women in Uganda.
Methods
This was a cross-sectional study conducted among 424 pregnant and lactating women, who were seeking maternal and child health care at Kawempe National Referral Hospital in Kampala. We obtained data on vaccine acceptability defined as willingness to accept vaccines using interviewer-administered questionnaires. In addition, we assessed vaccination status. Factors associated with COVID-19 vaccine acceptability and uptake were evaluated using modified Poisson regression.
Results
The mean age of the respondents was 26.9 years (SD = 5.7), ranging from 14–45 years. Among 424 respondents, 51.7% had received at least one dose of COVID-19 vaccine, 94.1% had received tetanus toxoid vaccine, and 48.3% regardless of their vaccination status, were willing to receive COVID-19 vaccines. Only 5/212(2.4%) had received COVID-19 vaccines while lactating with none having been received during pregnancy. Factors associated with COVID-19 vaccine uptake included history of testing for COVID-19 (aPR = 1.92, CI:1.46–2.54) and having a vaccinated household member (aPR = 1.34, CI:1.03–1.84). COVID-19 vaccine willingness was significantly associated with being a household head (aPR = 2.2, CI:1.12–4.27) and having a vaccinated household member (aPR = 1.33, CI:1.04–1.76).
Conclusion
The uptake and willingness to receive COVID-19 vaccines among pregnant and lactating women were generally low, with no participants receiving COVID-19 vaccines during pregnancy and very few during lactation. In contrast, the majority had received tetanus toxoid vaccines, indicating a disparity in vaccine acceptance. Factors such as undergoing COVID-19 testing and having a vaccinated household member positively influenced COVID-19 vaccine uptake and willingness. These findings highlight the need for targeted interventions to improve the acceptance and uptake of COVID-19 vaccines, during pregnancy and lactation.
Structural Dynamics of Human deoxyuridine 5'- triphosphate nucleotidohydrolase (dUTPase)
Authors: Sarre Ravdna , Dobrovolska Olena , Lundström Patrik , Turcu Diana , Agback Tatiana , Halskau Øyvind , Isaksson Johan 
Published: September 2024

Abstract

Structural- and functional heterogeneity, as well as allosteric regulation, in homo-monomeric enzymes is a highly active area of research. One such enzyme is human nuclear-associated deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase), which has emerged as an interesting drug target in combination therapy with traditional nucleotide analogue treatment of cancer. We report, for the first time, a full structural dynamics study of human dUTPase by NMR. dUTPase has been investigated in terms of structural dynamics in its apo form, in complex with the modified substrate resistant to hydrolysis, 2'-deoxyuridine 5'-α,β-imido-triphosphate (dUpNHpp), as well as the product, 2'-deoxy-uridine-monophosphate (UMP). The apo form of the enzyme displayed slow dynamics in the milli- to microsecond regime in relaxation dispersion experiments, which was further slowed down to observable heterogeneity upon substrate-analogue binding. The results suggest that the non-hydrolysable substrate-analogue traps the enzyme in the conformational isomerization step that has been previously suggested to be part of the enzyme catalysis kinetics cycle. The observed heterogeneity fits well with the pattern expected to emerge from the suggested kinetic model, and no evidence for homotropic allosterism was found. The heatmaps of the slow dynamics, chemical shift perturbation upon substrate binding and conserved regions of the enzyme sequence all displayed a similar pattern, which suggests that the structural dynamics is finely tuned and important for the biological function of the enzyme for binding, conformational shift, catalysis and substrate release.
FruR-controlled antisense RNA -downregulation of isocitrate dehydrogenase in Escherichia coli
Authors: Elias Jisha , Sharma Vikas , Archana G. , Kumar G. Naresh 
Published: September 2024

Abstract

In E. coli, catabolite repressor activator (Cra) protein (formerly called FruR) is known to regulate the expression of many genes positively and negatively; this effect is modulated by intracellular levels of fructose-1-phosphate (F-1-P) and fructose-1,6-bisphopahate (F-1,6-bisP). In this paper, we report conditionally expressed antisense RNA corresponding to 101bp of isocitrate dehydrogenase (icd) gene (as-icd) under Cra (FruR) responsive promoter fruB (PfruB as-icd construct denoted as pVS2K3) in E. coli K-12 (DH5α) and E. coli B (BL21) strains. Previously studies have shown that ICDH mutants failed to grow on glucose in absence of glutamate and accumulated citrate intracellularly. Hence, a conditional downregulation of icd gene could overcome this lethality and also help in understanding the flux towards citrate accumulation. Effect of PfruB as-icd (pVS2k3) construct was monitored in E. coli K-12 (DH5α) and E. coli B (BL21) during growth on carbon sources wherein the fruB promoter is active (glucose) or repressed (glycerol). A 3–4 fold decrease in ICDH activity was observed in E. coli DH5α expressing pVS2K3 on glucose but PfruB as-icd expression differed in E. coli BL21 on glucose. This alteration could be attributed to the anomalous Cra regulation seen in E. coli B strain which could be a crucial factor while choosing fruB promoter for expression studies.
Two squaramide-based fluorescent probes for Cu2+ and Cd2+
Authors: Li Yuanwei , Wang Hongxu , Tao Qian , Wang Bin 
Published: September 2024

Abstract

The development of potential toxic metal ion probes is of great significance in the field of environmental detection. Herein, two squaramide ligands (2a, 2b) were constructed by combining the characteristics of squaric acid and imine groups. 2a and 2b can recognize Cu2+ and Cd2+, with LOD of 1.26 × 10-8 M and 2.04 × 10-8 M, respectively, and have the advantages of fast response and wide pH range. The binding ratio and binding mode of the probe and the target ion were determined by Job’s plot, ESI-MS and 1H NMR.