bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence polarization control for on-off switching of single molecules at cryogenic temperatures
Authors: Hulleman Christiaan , Huisman Max , Moerland Robert , Grunwald David , Stallinga Sjoerd , Rieger Bernd 
Published: October 2017

AbstractLight microscopy allowing sub-diffraction limited resolution has been among the fastest developing techniques at the interface of biology, chemistry and physics. Intriguingly no theoretical limit exists on how far the underlying measurement uncertainty can be lowered. In particular data fusion of large amounts of images can reduce the measurement error to match the resolution of structural methods like cryo-electron microscopy. Fluorescence, although reliant on a reporter molecule and therefore not the first choice to obtain ultra resolution structures, brings highly specific labeling of molecules in a large assemble to the table and inherently allows the detection of multiple colors, which enable the interrogation of multiple molecular species at the same time in the same sample. Here we discuss the problems to be solved in the coming years to aim for higher resolution and describe what polarization depletion of fluorescence at cryogenic temperatures can contribute for fluorescence imaging of biological samples like whole cells.
Programmed hierarchical patterning of bacterial populations
Authors: Boehm Christian R. , Grant Paul K. , Haseloff Jim 
Published: October 2017

AbstractModern genetic tools allow the dissection and emulation of fundamental mechanisms shaping morphogenesis in multicellular organisms. Several synthetic genetic circuits for control of multicellular patterning have been reported to date. However, hierarchical induction of gene expression domains has received little attention from synthetic biologists, despite its importance in biological self-organization. We report the first synthetic genetic system implementing population-based AND logic for programmed autonomous induction of bacterial gene expression domains. We develop a ratiometric assay for bacteriophage T7 RNA polymerase activity and use it to systematically characterize different intact and split enzyme variants. We then utilize the best-performing variant to build a three-color patterning system responsive to two different homoserine lactones. We validate the AND gate-like behavior of this system both in cell suspension and in surface culture. Finally, we use the synthetic circuit in a membrane-based spatial assay to demonstrate programmed hierarchical patterning of gene expression across bacterial populations.Abbreviations3OC6HSLN-(3-oxohexanoyl)-L-homoserine lactone3OC12HSLN-(3-oxododecanoyl)-L-homoserine lactoneCFPcyan fluorescent proteinIPTGisopropyl-β-D-thiogalactopyranosideParts RegistryMIT Registry of Standard Biological PartsPIpositional informationRDreaction-diffusionRFPred fluorescent proteinRFUrelative fluorescence unitss.d.standard deviationT7RNAPbacteriophage T7 RNA polymeraseYFPyellow fluorescent protein
Storage Compartments for Capillary Water Rarely Refill in an Intact Woody Plant
Authors: Knipfer Thorsten , Cuneo Italo F. , Earles J. Mason , Reyes Clarissa , Brodersen Craig R. , McElrone Andrew J. 
Published: October 2017

Abstract
Water storage is thought to play an integral role in the maintenance of whole-plant water balance. The contribution of both living and dead cells to water storage can be derived from rehydration and water-release curves on excised plant material, but the underlying tissue-specific emptying/refilling dynamics remain unclear. Here, we used x-ray computed microtomography to characterize the refilling of xylem fibers, pith cells, and vessels under both excised and in vivo conditions in Laurus nobilis. In excised stems supplied with water, water uptake exhibited a biphasic response curve, and x-ray computed microtomography images showed that high water storage capacitance was associated with fiber and pith refilling as driven by capillary forces: fibers refilled more rapidly than pith cells, while vessel refilling was minimal. In excised stems that were sealed, fiber and pith refilling was associated with vessel emptying, indicating a link between tissue connectivity and water storage. In contrast, refilling of fibers, pith cells, and vessels was negligible in intact saplings over two time scales, 24 h and 3 weeks. However, those compartments did refill slowly when the shoot was covered to prevent transpiration. Collectively, our data (1) provide direct evidence that storage compartments for capillary water refill in excised stems but rarely under in vivo conditions, (2) highlight that estimates of capacitance from excised samples should be interpreted with caution, as certain storage compartments may not be utilized in the intact plant, and (3) question the paradigm that fibers play a substantial role in daily discharge/recharge of stem capacitance in an intact tree.
When does “liking” a charity lead to donation behaviour?
Authors: Wallace Elaine , Buil Isabel , de Chernatony Leslie 
Published: October 2017

Purpose
This study aims to investigate the relationship between young people’s Conspicuous Donation Behaviour (CDB) on social media platforms and their offline donation behaviour, specifically intentions to donate and volunteer time. It also explores materialism, self-esteem and self-monitoring as CDB trait antecedents, as a form of conspicuous consumption on social media. Finally, it considers the influence of altruism on these relationships.

Design/methodology/approach
A survey was conducted of regular Facebook users mentioning a charity brand on Facebook in the past year. Data from 234 participants were analysed and hypotheses tested using structural equation modeling.

Findings
Results confirm two forms of CDB – self and other-oriented. Materialistic consumers are more likely to engage in both forms of CDB on Facebook. High self-esteem increases self-oriented CDB; high self-monitoring increases other-oriented CDB. Self-oriented CDB is positively associated with donation intentions, but other-oriented CDB is negatively associated. Findings reveal how altruism moderates this model.

Research limitations/implications
Findings show how personality traits influence CDB and reveal the relationship between CDB, as virtual conspicuous consumption on social media platforms, and donation behaviour.

Practical implications
The study provides implications for managers about enhancing charitable donations through social media.

Originality/value
This is the first study to explore donation behaviour as a form of conspicuous consumption on social media, where virtual conspicuous consumption (i) does not require any offline consumption and (ii) may achieve the desired recognition, without any charitable act. It provides new insights into CDB, its antecedents and influence on donation behaviour.
Can political cookies leave a bad taste in one’s mouth?
Authors: Tal Aner , Gvili Yaniv , Amar Moty , Wansink Brian 
Published: October 2017

Purpose
This study aims to examine whether companies’ donations to political parties can impact product experience, specifically taste.

Design/methodology/approach
Research design consists of four studies; three online, one in person. Participants were shown a cookie (Studies 1-3) or cereal (Study 4) and told that the producing company donated to either the Republican Party or the Democratic Party (Studies 1-3) or an unspecified party (Study 4).

Findings
Participants rated food products as less tasty if told they came from a company that donated to a party they object to. These effects were shown to be mediated by moral disgust (Study 3). Effects were restricted to taste and willingness to buy (Study 4), with no effects on other positive product dimensions.

Research limitations/implications
The studies provide a first piece of evidence that political donations by companies can negatively impact product experience. This can translate to purchase decisions through an emotional, rather than calculated, route.

Practical implications
Companies should be careful about making donations some of their consumers may find objectionable. This might impact both purchase and consumption decisions, as well as post-consumption word-of-mouth.

Originality/value
Companies’ political involvement can negatively impact subjective product experience, even though such information has no bearing on product quality. The current findings demonstrate that alterations in subjective product quality may underlie alterations in consumer decision-making because of ideologically tinged information, and reveals moral disgust as the mechanism underlying these effects. In this, it provides a first demonstration that even mild ideological information that is not globally bad or inherently immoral can generate moral disgust, and that such effects depend on consumers’ own attitudes.
Diagnostic and prescriptive benefits of consumer participation in virtual communities of personal challenge
Authors: Lowe Ben , Johnson Devon 
Published: October 2017

Purpose
The purpose of this paper is to show how active participants within personal challenge virtual communities (e.g. virtual health communities, online legal forums, etc.) derive learning benefits from their involvement within the community. In doing so, the research conceptualises and tests a model of engagement within such virtual communities.

Design/methodology/approach
This research was conducted through the design of a survey administered to an online panel of active participants from several virtual health communities. Structural equation modelling was used to test the conceptual model.

Findings
Along with well-researched concepts such as social identification, this research identifies diagnostic and prescriptive benefits as key learning benefits associated with active participation within personal challenge communities. These benefits drive social support which individuals attain from these virtual communities, which, in turn, drives engagement within the community. It is also found that anticipated negative emotions from leaving the community mediate social support and engagement.

Originality/value
This is one of the first studies to develop a model of consumer engagement with personal challenge virtual communities. The findings make a contribution to the field of online communities by showing how learning benefits (diagnostic and prescriptive) transpire within these communities and how these benefits lead to greater community engagement.
The asymmetric impact of other-blame regret versus self-blame regret on negative word of mouth
Authors: Wu Ruijuan , Wang Cheng Lu 
Published: October 2017

Purpose
This study aims to distinguish two regret conditions, other-blame regret (O-regret) and self-blame regret (S-regret), and investigate the underlying mechanism and boundary conditions of the relationship between regret and negative word of mouth (NWOM).

Design/methodology/approach
Four experiments and one survey study test hypotheses regarding how O-regret and S-regret influence NWOM through mediating mechanism of anger and sadness and how the impact of regret on NWOM is moderated by boundary conditions.

Findings
The results show that consumers who experience O-regret transmit more NWOM than those who experience S-regret. Anger is a dominant emotion when consumers experience O-regret and mediates the impact of regret on NWOM, and sadness is a dominant emotion when consumers experience S-regret and mediates the impact of regret on NWOM. In addition, purchased price (full vs discount price), regret context (private vs public context) and return policy (strict vs lenient policy) are found to moderate the effect of regret on NWOM.

Research limitations/implications
This study was conducted in China, which has a unique business environment that may differ from other countries. Therefore, this research opens a new avenue to further examine such a phenomenon in countries where a more lenient return policy is a standard business practice. Cross-nation studies comparing how different return policies and other business environment conditions are warranted in future research.

Practical implications
The study provides several insights for marketers considering the management of NWOM by understanding consumer O-regret and S-regret in either online or offline retailing situations.

Originality/value
This paper contributes to the extant literature by distinguishing different outcome regrets. The theoretical conceptualization and empirical findings shed further lights on the relationship between regret and other negative emotions and how O-regret and S-regret lead to different impacts on NWOM through different paths of mediation mechanism.
Corporate heritage brands, augmented role identity and customer satisfaction
Authors: Balmer John M.T. , Chen Weifeng 
Published: October 2017

PurposeThe study aims to explore customer satisfaction towards the celebrated Tong Ren Tang (TRT) Chinese corporate heritage brand (established in 1669). This paper examines the multiple role identities of the corporate brand and, in particular, the enduring imperial identity (role identity) of the corporate brand. The study examines whether the corporate heritage brand’s imperial associations are still meaningful.Design/methodology/approachA indicative, survey-based case study methodology undertaken with Chinese customers informs this research.FindingsTRT’s corporate heritage brand identity and, moreover, its imperial role identity were salient in terms of customer satisfaction. TRT’s augmented imperial role identity not only was highly salient but also, moreover, meaningfully enhanced the organisation’s corporate reputation in terms of customer satisfaction.Research limitations/implicationThis study lends further support for the utility of the notion of corporate heritage/corporate heritage brands and in particular the saliency of the theoretical notion of augmented role identity within the corporate heritage marketing field.Practical implicationCorporate heritage brand managers should be appraised of which corporate role identities are meaningful for customers. At a practical level, senior corporate marketing managers of corporate heritage organisations should accorded importance to the additional P of Provenance apropos the corporate marketing mix.Social implicationAt a time, when China is reappraising its relationship with its past – including its imperial past (of which much has been destroyed) – this paper’s focus on TRT’s unsurpassed augmented role identity is pertinent and propitious. Seemingly, this corporate heritage brand’s imperial association provides a living and tangible link with China’s long and momentous imperial provenance and erstwhile imperial polity. In short, the corporate heritage brand is part of China’s patrimony and enjoys a unique place in this regard.Originality/valueThis paper is one of the first empirical studies examining a Chinese corporate heritage brand entity. The study marks new ground in examining customer satisfaction from the theoretical perspectives of corporate heritage brand and augmented role identity. It is believed that this is the first study to consider corporate heritage in the pharmaceutical sector and marks new ground in considering the saliency of China’s imperial legacy on an extant, highly successful and high profile-Chinese corporate heritage brand.
Differences in symbolic self-completion and self-retention across role-identity cultivation stages
Authors: Kleine Robert E. , Kleine Susan Schultz , Ewing Douglas R. 
Published: October 2017

PurposeThis paper aims to provide evidence that theory-based effects of role-identity cultivation stages on self-symbolizing consumption activities do exist.Design/methodology/approachSpecific focus is placed upon differing motives between rookie versus veteran role-identity actors and how these differences lead to symbolic self-completion and self-retention behaviors. Effects of these motives are examined in the context of college student identity transitions.FindingsEvidence is found for a pattern, whereby role-identity rookies with fewer role-identity-related possessions are more likely to self-symbolize the role-identity outwardly than veteran consumers having more role-identity-related resources, such as possessions. Self-retention via possessions is also more evident with rookies making the transition from one role-identity to the next, replacement role-identity. Findings are replicated for both readily available and favorite possessions related to a role-identity.Research limitations/implicationsFuture role-identity research in marketing may miss unique and important insights without accounting for role-identity cultivation stage.Practical implicationsCurrent evidence highlights the importance of identity cultivation stage, symbolic self-completion and self-retention as factors to consider in understanding market segments associated with respective role-identities.Originality/valueExtant research does not yet account for how consumption activities serving both symbolic and functional purposes support role-identity transitions. This inquiry is directed at contributing to this need.
Reflections on a decade of EJM and marketing scholarship: the good, the bad, and the future
Authors: Lee Nick 
Published: October 2017

PurposeThe purpose of this paper is to reflect on the author’s decade-long tenure as the Editor of theEuropean Journal of Marketing(EJM). The paper presents his thoughts on the past 10 years of marketing scholarship, his views on future directions and some advice for those looking to publish their research in academic journals.Design/methodology/approachThe paper takes a reflective, discursive approach, and also reviews a wide range of topics relevant to marketing researchers.FindingsThe author finds thatEJMhas grown substantially on many levels in the past decade. He also finds that there are some concerns around marketing research, and social scientific scholarship in general, that marketing scholars may wish to consider and take into account in their ongoing work.Research limitations/implicationsThe paper is partly a personal view, and does not rely on any empirical research. However, the views espoused are justified by theoretical review and conceptual argument.Practical implicationsThe implications of this paper are relevant to marketing scholars, journal reviewers, readers of research, as well as those who manage scholarship (e.g. university administrators). The author suggests a number of directions that the research, publication and reward process could move in to improve practice.Originality/valueThe paper brings together a large number of different views and concepts relevant to further development of marketing research, and provides original summaries and extensions.
High-fit charitable initiatives increase hedonic consumption through guilt reduction
Authors: Baghi Ilaria , Antonetti Paolo 
Published: October 2017

Purpose
Past research on cause-related marketing (CRM) suggests that these socially beneficial initiatives are more effective when linked with hedonic than utilitarian products. Little is known, however, about the process underpinning this effect. This paper aims to investigate why and under what circumstances CRM enhances the appeal of hedonic products by testing the mediation of guilt and introducing the moderating role of cause-product fit.

Design/methodology/approach
The authors test a model of moderated mediation in two studies. Study 1 shows that the effectiveness of combining CRM with hedonic consumption is explained by the mediating role of feelings of guilt. Study 2 demonstrates that this mediation depends on the level of fit or congruency between the cause and the product.

Findings
Results suggest that CRM campaigns offer the opportunity to improve the consumption experiences of hedonic products by reducing the feelings of guilt intrinsically connected with these options. Moreover, fit moderates the emotional processes activated by CRM initiatives. When fit is high, CRM reduces guilt and improves consumers’ experiences when purchasing hedonic alternatives.

Originality/value
The study extends current understanding of how CRM can promote hedonic consumption and contributes further to research on guilt as an emotion able to promote responsible consumption decisions. Moreover, the study introduces and tests the impact of cause-product fit in predicting consumers’ ethical purchase intention. For managers of hedonic brands, the study offers important implications on how to deploy CRM campaigns to foster better customer experiences.
BIM and sensor-based data management system for construction safety monitoring
Authors: Riaz Zainab , Parn Erika A. , Edwards David J. , Arslan Muhammad , Shen Charles , Pena-Mora Feniosky 
Published: October 2017

Purpose
This research aims to investigate the integration of real-time monitoring of thermal conditions within confined work environments through wireless sensor network (WSN) technology when integrated with building information modelling (BIM). A prototype system entitled confined space monitoring system (CoSMoS), which provides an opportunity to incorporate sensor data for improved visualization through new add-ins to BIM software, was then developed.

Design/methodology/approach
An empirical study was undertaken to compare and contrast between the performances (over a time series) of various database models to find a back-end database storage configuration that best suits the needs of CoSMoS.

Findings
Fusing BIM data with information streams derived from wireless sensors challenges traditional approaches to data management. These challenges encountered in the prototype system are reported upon and include issues such as hardware/software selection and optimization. Consequently, various database models are explored and tested to find a database storage that best suits the specific needs of this BIM-wireless sensor technology integration.

Originality value
This work represents the first tranche of research that seeks to deliver a fully integrated and advanced digital built environment solution for automating the management of health and safety issues on construction sites.
A systematic approach for enterprise systems upgrade decision-making
Authors: Feldman Gerald , Shah Hanifa , Chapman Craig , Pärn Erika A. , Edwards David J. 
Published: October 2017

Purpose
Enterprise systems (ES) upgrade is fundamental to maintaining a system’s continuous improvement and stability. However, while the extant literature is replete with research on ES upgrade decision-making, there is scant knowledge about how different decision processes facilitate this decision to upgrade. This paper aims to investigate and better understand these processes from an organisational perspective.

Design/methodology/approach
The study adopted a qualitative survey design, and used a Web-based questionnaire and semi-structured interviews to collect data from 23 large organisations. Data accrued were qualitatively analysed and manually coded to identify the various decision processes undertaken during ES upgrade decisions.

Findings
Analysis results reveal complex interrelations between the upgrade drivers, the need to evaluate the new version’s functionality and the upgrade impact. Understanding the interaction between these elements influences the upgrade decision process.

Research limitations/implications
The study proposes ES upgrade processes that support a decision to upgrade major releases. Further research is required to offer either similar or conflicting arguments on the upgrade decision-making and provide a probabilistic generalisation of the decision-making processes.

Originality/value
The research offers a comprehensive and empirically supported methodical approach that embraces an evaluation of a new version’s functionality, technical requirements and concomitant upgrade implications as intrinsic decision processes. This approach assists in the decisions to establish the upgrade need and determine the level of change, effort required, impacts and associated benefits.