bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of cystic echinococcosis in Iran: a systematic review and meta-analysis
Authors: Khalkhali H.R. , Foroutan M. , Khademvatan S. , Majidiani H. , Aryamand S. , Khezri P. , Aminpour A. 
Published: June 2017

AbstractAs a significant zoonosis, cystic echinococcosis (CE) is endemic in some parts of the world, such as the Middle East. There are studies on the prevalence of this infection in animal and human reservoirs in Iran; hence, we conducted this meta-analysis to elucidate the prevalence of CE in Iran. English (PubMed, Scopus, Web of Science, Science Direct and Google Scholar) and Persian (Magiran, Iran Medex, Iran Doc and SID) databases were explored. In the case of definitive, animal and human intermediate hosts, 37, 90 and 33 studies, respectively, have been included in the current review from January 1990 to December 2015. According to outcomes of the heterogeneity test, either Der Simonian and Laird's random-effects method or Mantel–Haenszel's fixed-effects method were employed to pool the estimations. The pooled prevalence ofEchinococcus granulosusinfection in definitive hosts was calculated as 23.6% (95% confidence interval (CI) = 17.6–30.1%). The weighted prevalence of animal and human hydatidosis was calculated as 15.6% (95% CI = 14.2–17.1%) and 4.2% (95% CI = 3.0–5.5%), respectively. Meanwhile, most cases of human hydatidosis were in southern Iran, with a prevalence of 5.8% (3.2–9.2%). In terms of human hydatidosis, more infections were found in rural regions, and mostly in female individuals. Egger's regression test revealed publication bias, with a remarkable impact on total prevalence of the infection in animal intermediate hosts (P< 0.001), while it was not significant in human hosts (P= 0.4) and definitive hosts (P= 0.3). According to the weighted estimated prevalence of cystic echinococcosis and its financial burden, implementing appropriate control programmes should be compulsory to decrease the burden of the disease in Iran.
Developing a carer identity and negotiating everyday life through social networking sites: an explorative study on identity constructions in an online Swedish carer community
Authors: ANDRÉASSON FRIDA , ANDREASSON JESPER , HANSON ELIZABETH 
Published: June 2017

ABSTRACTAn overarching reason why carers do not utilise support services is that many people who perform care-giving do not necessarily self-identify as a carer. Understanding the development of carer identities is therefore crucial for the utilisation of different carer-focused health services. This study arose from the European Union-funded INNOVAGE project and aimed to describe how older carers conceptualise and understand their identity as carers on a Swedish online social forum. Theoretically the study adopts a constructionist approach and the method of netnography was applied. The findings reveal that a change in self-perception occurs in the process through which a carer role is acquired. The presence or absence of recognition for the older carers’ capacity, knowledge and life situation is seen as filtered through the needs of the care recipient, making the carer identity into an invisible self. This is not least the case when the identity is constructed in alliance with conceptual and moral obligations found within a marital discourse. Nevertheless, the opportunity for online communication may help to create a virtual space of social recognition through which different experiences attached to caring can be discussed. The significance of online communication is here understood as the possibility it presents for carers to be recognised by other carers. It is a process through which an invisible self can become visible.
The effect of kinetic heat shock on bovine oocyte maturation and subsequent gene expression of targeted genes
Authors: Pavani Krishna C. , Rocha António , Baron Erica , Lourenço Joana , Faheem Marwa , da Silva Fernando Moreira 
Published: June 2017

SummaryThe exposure of oocytes to heat stress during the maturation process results in harmful effects to their internal organelles, low fertilization capability and higher embryonic losses. In the present experiment the effect of heat shock (HS) during the maturation process was assessed. In Assay 1, oocytes from winter (December–March; n = 100) and summer (June–September; n = 100) months were collected and matured to analyse their HS tolerance. Total RNA was extracted from matured oocytes and cDNA synthesis was performed, followed by qPCR for selected genes (Cx43, CDH1, DNMT1, HSPA14), compared with two reference genes (GAPDH and SDHA). In Assay 2, oocytes collected during the winter were subjected to kinetic HS by stressing them at 39.5°C for 6, 12, 18 or 24 h and were afterwards matured at control temperature (38.5°C), and subsequently subjected to the previously described gene analysis procedure. Results of Assay 1 show that summer-collected oocytes exhibited lower maturation rate than winter-collected oocytes, which may be due to the down-regulation of the HSPA 14 gene. Assay 2 showed that 6 h of HS had no effect on gene regulation. CDH1 and DNMT1 up-regulation was observed starting at 12 h, which may represent the effect of heat shock on oocyte development.
Prenatal diagnosis of isolated total anomalous systemic venous connection to the left atrium
Authors: Miltner Béatrice , Poncelet Alain Jean , Barrea Catherine 
Published: June 2017

AbstractWe report the prenatal diagnosis and the neonatal follow-up of a patient with isolated total abnormal systemic venous connection to the left atrium. Right-sided and left-sided superior caval veins and the inferior caval vein were all connected to the left atrium. Pulmonary venous return was normal. This was associated with some right ventricular underdevelopment. To our knowledge, this is the first fetal description of this very rare congenital cardiac malformation.
Content analysis of targeted food and beverage advertisements in a Chinese-American neighbourhood
Authors: Bragg Marie A , Pageot Yrvane K , Hernández-Villarreal Olivia , Kaplan Sue A , Kwon Simona C 
Published: June 2017

AbstractObjectivesThe current descriptive study aimed to: (i) quantify the number and type of advertisements (ads) located in a Chinese-American neighbourhood in a large, urban city; and (ii) catalogue the targeted marketing themes used in the food/beverage ads.DesignTen pairs of trained research assistants photographed all outdoor ads in a 0·6 mile2 (1·6 km2) area where more than 60·0 % of residents identify as Chinese American. We used content analysis to assess the marketing themes of ads, including references to: Asian cultures; health; various languages; children; food or beverage type (e.g. sugar-sweetened soda).SettingLower East Side, a neighbourhood located in the borough of Manhattan in New York City, USA.SubjectsAds (n 1366) in the designated neighbourhood.ResultsFood/beverage ads were the largest ad category (29·7 %, n 407), followed by services (e.g. mobile phone services; 21·0 %, n 288). Sixty-seven per cent (66·9 %) of beverages featured were sugar-sweetened, and 50·8 % of food ads promoted fast food. Fifty-five per cent (54·9 %) of food/beverage ads targeted Asian Americans through language, ethnicity of person(s) in the ad or inclusion of culturally relevant images. Fifty per cent (50·2 %) of ads were associated with local/small brands.ConclusionsFood/beverage marketing practices are known to promote unhealthy food and beverage products. Research shows that increased exposure leads to excessive short-term consumption among consumers and influences children’s food preferences and purchase requests. Given the frequency of racially targeted ads for unhealthy products in the current study and increasing rates of obesity-related diseases among Asian Americans, research and policies should address the implications of food and beverage ads on health.
Whole grain intake of Australians estimated from a cross-sectional analysis of dietary intake data from the 2011–13 Australian Health Survey
Authors: Galea Leanne M , Beck Eleanor J , Probst Yasmine C , Cashman Chris J 
Published: June 2017

AbstractObjectiveThe Australian Dietary Guidelines recommend Australians choose mostly whole-grain and/or high-fibre varieties within the grains (cereal) foods category, with other groups specifying a whole grain Daily Target Intake of 48 g for Australians aged 9 years or above. The USA and UK report estimates of whole grain intake that are low and declining, and no comprehensive studies on whole grain intake in the Australian population are available. The present study aimed to determine national estimates of whole grain intake, compared with current recommendations.DesignA recently updated whole grain database was applied to the most current population dietary intake data. Single 24 h dietary recall intake data were reviewed against age group, sex, relative to energy intake and whole grain recommendations.SettingAustralia.SubjectsAustralians (2–85 years) participating in the 2011–13 Australian Health Survey (n 12 153).ResultsThe median daily whole grain intake was 21 g for adults (19–85 years) and 17 g for children/adolescents (2–18 years), or 28 and 23 g/10 MJ per d, respectively. Approximately 30 % of children/adolescents consumed no whole grains on the day of the survey. Whole grain intake was lowest for the age group 14–18 years (8·7 g/d). Of all participants aged ≥9 years, 73 % did not reach the recommended Daily Target Intake of 48 g.ConclusionsWhole grain intake in Australia is below recommendations in all age groups. Adolescents may be a key target for campaigns to increase whole grain consumption. This study provides the first quantification of absolute whole grain intake from all food sources in a national sample of Australians.
Who's afraid of Allan Savory? Scientometric polarization on Holistic Management as competing understandings
Authors: Sherren Kate , Kent Carlisle 
Published: June 2017

AbstractHow to graze livestock sustainably is an important and complex question. The debate between rotational and continuous grazing has been ongoing since the 1950s, yet evidence is perennially mixed. We used scientometrics to understand the structure of science on Holistic Management (HM), the most contentious of these adaptive practices. We used papers in Web of Science since 1980 citing the work of HM's ‘father’, Allan Savory, as a way of delineating a field that is otherwise chaotic with terminology. Results show an increasingly diverse use of Savory's work geographically and in terms of subject areas. Taking a positive position on HM seems most likely for those doing farm-scale (rather than experimental) work in dry climates. Bibliographic factions align with the various disciplines working on grazing research and also their expressed opinion on HM practices. Factions represent disciplinary strength, suggesting barriers for integrative work but also the need for the resolution of competing understandings in specific contexts with diverse participants to inform grazing decisions.
EIF3D promotes gallbladder cancer development by stabilizing GRK2 kinase and activating PI3K-AKT signaling pathway
Authors: Zhang Fei , Xiang Shanshan , Cao Yang , Li Maolan , Ma Qiang , Liang Haibin , Li Huaifeng , Ye Yuanyuan , Zhang Yijian , Jiang Lin , Hu Yunping , Zhou Jian , Wang Xuefeng , Zhang Yong , Nie Lei , Liang Xiao , Gong Wei , Liu Yingbin 
Published: June 2017

AbstractRecent evidence suggests that dysregulated eIF3d expression may be critical in various genetic disorders as well as cancer. In this study, we observed that EIF3d levels increased in gallbladder cancer (GBC) samples compared with non-tumor tissue. High eIF3d levels were associated with advanced tumor stage and metastasis and were correlated with poor prognosis in 92 patients with GBC. Depletion of EIF3d in GBC cell lines inhibited cell proliferation, colony formation and metastasis and induced apoptosis and cell cycle arrest in vitro and in vivo. In contrast, ectopic expression of eIF3d had the opposite effects. Moreover, in this study, we revealed that a novel non-translational factor function of eIF3d mediated its protumoral effects. In details, eIF3d stabilizes GRK2 protein by blocking ubiquitin-mediated degradation, consequently activates PI3K/Akt signaling, and promotes GBC cell proliferation and migration. In conclusion, eIF3d promotes GBC progression mainly via eIF3d–GRK2–AKT axis and it may be used as a prognostic factor. The therapeutic targeting of eIF3d–GRK2 axis may be a potential treatment approach for GBC.
A BaSiC tool for background and shading correction of optical microscopy images
Authors: Peng Tingying , Thorn Kurt , Schroeder Timm , Wang Lichao , Theis Fabian J. , Marr Carsten , Navab Nassir 
Published: June 2017

AbstractQuantitative analysis of bioimaging data is often skewed by both shading in space and background variation in time. We introduce BaSiC, an image correction method based on low-rank and sparse decomposition which solves both issues. In comparison to existing shading correction tools, BaSiC achieves high-accuracy with significantly fewer input images, works for diverse imaging conditions and is robust against artefacts. Moreover, it can correct temporal drift in time-lapse microscopy data and thus improve continuous single-cell quantification. BaSiC requires no manual parameter setting and is available as a Fiji/ImageJ plugin.
Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information
Authors: Horejs Christine-Maria , St-Pierre Jean-Philippe , Ojala Juha R. M. , Steele Joseph A. M. , da Silva Patricia Barros , Rynne-Vidal Angela , Maynard Stephanie A. , Hansel Catherine S. , Rodríguez-Fernández Clara , Mazo Manuel M. , You Amanda Y. F. , Wang Alex J. , von Erlach Thomas , Tryggvason Karl , López-Cabrera Manuel , Stevens Molly M. 
Published: June 2017

AbstractMatrix metalloproteinases (MMPs) contribute to the breakdown of tissue structures such as the basement membrane, promoting tissue fibrosis. Here we developed an electrospun membrane biofunctionalized with a fragment of the laminin β1-chain to modulate the expression of MMP2 in this context. We demonstrate that interfacing of the β1-fragment with the mesothelium of the peritoneal membrane via a biomaterial abrogates the release of active MMP2 in response to transforming growth factor β1 and rescues tissue integrity ex vivo and in vivo in a mouse model of peritoneal fibrosis. Importantly, our data demonstrate that the membrane inhibits MMP2 expression. Changes in the expression of epithelial-to-mesenchymal transition (EMT)-related molecules further point towards a contribution of the modulation of EMT. Biomaterial-based presentation of regulatory basement membrane signals directly addresses limitations of current therapeutic approaches by enabling a localized and specific method to counteract MMP2 release applicable to a broad range of therapeutic targets.
Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
Authors: Xu Wei , Luthra Priya , Wu Chao , Batra Jyoti , Leung Daisy W. , Basler Christopher F. , Amarasinghe Gaya K. 
Published: June 2017

AbstractEbola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30–eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30–eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.
MYC activation cooperates with Vhl and Ink4a/Arf loss to induce clear cell renal cell carcinoma
Authors: Bailey Sean T. , Smith Aleisha M. , Kardos Jordan , Wobker Sara E. , Wilson Harper L. , Krishnan Bhavani , Saito Ryoichi , Lee Hyo Jin , Zhang Jing , Eaton Samuel C. , Williams Lindsay A. , Manocha Ujjawal , Peters Dorien J. , Pan Xinchao , Carroll Thomas J. , Felsher Dean W. , Walter Vonn , Zhang Qing , Parker Joel S. , Yeh Jen Jen , Moffitt Richard A. , Leung Janet Y. , Kim William Y. 
Published: June 2017

AbstractRenal carcinoma is a common and aggressive malignancy whose histopathogenesis is incompletely understood and that is largely resistant to cytotoxic chemotherapy. We present two mouse models of kidney cancer that recapitulate the genomic alterations found in human papillary (pRCC) and clear cell RCC (ccRCC), the most common RCC subtypes. MYC activation results in highly penetrant pRCC tumours (MYC), while MYC activation, when combined with Vhl and Cdkn2a (Ink4a/Arf) deletion (VIM), produce kidney tumours that approximate human ccRCC. RNAseq of the mouse tumours demonstrate that MYC tumours resemble Type 2 pRCC, which are known to harbour MYC activation. Furthermore, VIM tumours more closely simulate human ccRCC. Based on their high penetrance, short latency, and histologic fidelity, these models of papillary and clear cell RCC should be significant contributions to the field of kidney cancer research.
Insights into Hunter syndrome from the structure of iduronate-2-sulfatase
Authors: Demydchuk Mykhaylo , Hill Chris H. , Zhou Aiwu , Bunkóczi Gábor , Stein Penelope E. , Marchesan Denis , Deane Janet E. , Read Randy J. 
Published: June 2017

AbstractHunter syndrome is a rare but devastating childhood disease caused by mutations in the IDS gene encoding iduronate-2-sulfatase, a crucial enzyme in the lysosomal degradation pathway of dermatan sulfate and heparan sulfate. These complex glycosaminoglycans have important roles in cell adhesion, growth, proliferation and repair, and their degradation and recycling in the lysosome is essential for cellular maintenance. A variety of disease-causing mutations have been identified throughout the IDS gene. However, understanding the molecular basis of the disease has been impaired by the lack of structural data. Here, we present the crystal structure of human IDS with a covalently bound sulfate ion in the active site. This structure provides essential insight into multiple mechanisms by which pathogenic mutations interfere with enzyme function, and a compelling explanation for severe Hunter syndrome phenotypes. Understanding the structural consequences of disease-associated mutations will facilitate the identification of patients that may benefit from specific tailored therapies.
Local particle-hole pair excitations by SU(2) symmetry fluctuations
Authors: Montiel X. , Kloss T. , Pépin C. 
Published: June 2017

AbstractUnderstanding the pseudo-gap phase which opens in the under-doped regime of cuprate superconductors is one of the most enduring challenges of the physics of these compounds. A depletion in the electronic density of states is observed, which is gapping out part of the Fermi surface, leading to the formation of mysterious lines of massless excitations- the Fermi arcs. Here we give a new theoretical account of the physics of the pseudo-gap phase in terms of the emergence of local patches of particle-hole pairs generated by SU(2) symmetry fluctuations. The proliferation of these local patches accounts naturally for the robustness of the pseudo-gap phase to disturbances like disorder or magnetic field and is shown to gap out part of the Fermi surface, leading to the formation of the Fermi arcs. Most noticeably, we show that these patches induce a modulated charge distribution on the Oxygen atoms, in remarkable agreement with recent X-ray and STM observations.
Non-muscle myosin II is required for correct fate specification in the Caenorhabditis elegans seam cell divisions
Authors: Ding Siyu Serena , Woollard Alison 
Published: June 2017

AbstractDuring development, cell division often generates two daughters with different developmental fates. Distinct daughter identities can result from the physical polarity and size asymmetry itself, as well as the subsequent activation of distinct fate programmes in each daughter. Asymmetric divisions are a feature of the C. elegans seam lineage, in which a series of post-embryonic, stem-like asymmetric divisions give rise to an anterior daughter that differentiates and a posterior daughter that continues to divide. Here we have investigated the role of non-muscle myosin II (nmy-2) in these asymmetric divisions. We show that nmy-2 does not appear to be involved in generating physical division asymmetry, but nonetheless is important for specifying differential cell fate. While cell polarity appears normal, and chromosome and furrow positioning remains unchanged when nmy-2 is inactivated, seam cell loss occurs through inappropriate terminal differentiation of posterior daughters. This reveals a role for nmy-2 in cell fate determination not obviously linked to the primary polarity determination mechanisms it has been previously associated with.
Pharmacodynamic and pharmacokinetic assessment of pulmonary rehabilitation mixture for the treatment of pulmonary fibrosis
Authors: Zhao Juanjuan , Ren Yan , Qu Yubei , Jiang Wanglin , Lv Changjun 
Published: June 2017

AbstractPulmonary rehabilitation mixture (PRM), a Chinese herbal medicine formula, has been used to treat pulmonary fibrosis for decades. In this study, we systematically evaluated the pharmacodynamic and pharmacokinetic performance of PRM. The pharmacodynamic results showed that PRM could improve the condition of CoCl2-stimulated human type II alveolar epithelial cells, human pulmonary microvascular endothelial cells, human lung fibroblasts and pulmonary fibrosis rats induced by bleomycin, PRM treatment reduced the expression of platelet-derived growth factor, fibroblast growth factor, toll-like receptor 4, high-mobility group box protein 1 and hypoxia-inducible factor 1α. In the pharmacokinetic study, an accurate and sensitive ultra-high performance liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of calycosin, calycosin-7-O-glucoside, formononetin, ononin and mangiferin of PRM in the rat plasma for the first time. The method was then successfully applied to the comparative pharmacokinetic study of PRM in normal and pulmonary fibrosis rats. The five constituents could be absorbed in the blood after the oral administration of PRM and exhibited different pharmacokinetic behaviors in normal and pulmonary fibrosis rats. In summary, PRM exhibited a satisfactory pharmacodynamic and pharmacokinetic performance, which highlights PRM as a potential multi-target oral drug for the treatment of pulmonary fibrosis.