bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: The significance of the change pattern of serum CA125 level for judging prognosis and diagnosing recurrences of epithelial ovarian cancer
Authors: Yang Zhi-jun , Zhao Bing-bing , Li Li 
Published: September 2016

Abstract
Background
Ovarian cancer has the highest mortality rate of the three main malignant tumors of the female reproductive system, with a 5-year overall survival (OS) of only 20–30 %. Approximately 70 % of patients relapse without being cured. To explore the significance of serum CA125 level pre-treatment and the change pattern of CA125 post-treatment for judging prognosis and diagnosing recurrences of epithelial ovarian cancer (EOC).

Methods
A radioimmunoassay was used to continuously monitor levels of serum CA125 in 152 patients with EOC. The first test was done before surgery, then once a month after surgery for more than two consecutive years. The data were analyzed by using Kaplan-Meier curves and the log-rank test, stratified chi-square test, Pearson correlation analysis, and multivariate Cox regression analysis.

Results
(1) There was a relationship between patient outcomes and the serum CA125 levels before treatment and the extent and speed of serum CA125 decrease after treatment. The outcomes of patients with pre-treatment serum CA125 ≤ 35 U/ml were better than those with serum CA125 > 35 U/ml; the outcomes of patients with serum CA125 who had a logarithmic decrease or a decrease to normal within a month after treatment were also better than those with a non-logarithmic decrease or a decrease to normal that took longer than a month. (2) The results of multivariate Cox regression analysis showed that serum CA125 levels before treatment and a decreased speed of decline after treatment were independent prognostic factors; (3) The mean level of serum CA125 at relapse was 116.28 U/ml. The average time from serum CA125 increase to detection of a recurrent lesion by physical or imaging examination was 122 days. The correlation coefficient of serum CA125 level increase and tumor recurrence time was −0.674. (4) The area under the Receiver Operating Characteristic (ROC) curve of serum CA125 for diagnosing EOC recurrence was 0.879, and the sensitivity and specificity were 67.39 and 86.79 %, respectively.

Conclusions
It is important to monitor serum CA125 levels pre-treatment and the change pattern of CA125 post-treatment for judging prognosis and diagnosing recurrences of EOC.
Technical Advance: Changes in neutrophil migration patterns upon contact with platelets in a microfluidic assay
Authors: Frydman Galit H , Le Anna , Ellett Felix , Jorgensen Julianne , Fox James G , Tompkins Ronald G , Irimia Daniel 
Published: September 2016

Abstract
Neutrophils are traditionally regarded as the “first responders” of the immune system. However, recent observations revealed that platelets often respond earlier to recruit and activate neutrophils within sites of injury and inflammation. Currently, platelet–neutrophil interactions are studied by intravital microscopy. Although such studies provide exceptional, physiologic in vivo data, they are also laborious and have low throughput. To accelerate platelet–neutrophil interaction studies, we have developed and optimized an ex vivo microfluidic platform with which the interactions between platelets and moving neutrophils are measured at single-cell level in precise conditions and with high throughput. With the use of this new assay, we have evaluated changes in neutrophil motility upon direct contact with platelets. Motility changes include longer distances traveled, frequent changes in direction, and faster neutrophil velocities compared with a standard motility response to chemoattractant fMLP. We also found that the neutrophil–platelet direct interactions are transient and mediated by CD62P–CD162 interactions, localized predominantly at the uropod of moving neutrophils. This “crawling,” oscillatory neutrophil behavior upon platelet contact is consistent with previous in vivo studies and validates the use of this new test for the exploration of this interactive relationship.
Regulation of FcRγ function by site-specific serine phosphorylation
Authors: Shah Spandan , Gibson Andrew W , Ji Chuanyi , Darrington Eric , Mobley James , Kojima Kyoko , Edberg Jeffrey C , Kimberly Robert P 
Published: September 2016

Abstract
The common FcRγ, an immunoreceptor tyrosine-based activation motif (ITAM)- containing adaptor protein, associates with multiple leukocyte receptor complexes and mediates signal transduction through the ITAM in the cytoplasmic domain. The presence of multiple serine and threonine residues within this motif suggests the potential for serine/threonine phosphorylation in modulating signaling events. Single-site mutational analysis of these residues in RBL-2H3 cells indicates that each may contribute to net FcRγ-mediated signaling, and mass spectrometry of WT human FcRγ from receptor-stimulated cells shows consistent preferential phosphorylation of the serine residue at position 51. Immunoblot analysis, mass spectrometry, and mutational analyses showed that phosphorylation of serine 51 in the 7-residue spacer between the 2 YxxL sequences regulates FcRγ signaling by inhibiting tyrosine phosphorylation at the membrane proximal Y47 position of the ITAM, but not phosphorylation at position Y58. This inhibition results in reduced Syk recruitment and activation. With in vitro kinase assays, PKC-δ and PKA show preferential phosphorylation of S51. Serine/threonine phosphorylation of the FcRγ ITAM, which functions as an integrator of multiple signaling elements, may explain in part the contribution of variants in PKC-δ and other PKC isoforms to some autoimmune phenotypes.
Lipoxin A4, a 5-lipoxygenase pathway metabolite, modulates immune response during acute respiratory tularemia
Authors: Singh Anju , Rahman Tabassum , Bartiss Rose , Arabshahi Alireza , Prasain Jeevan , Barnes Stephen , Musteata Florin Marcel , Sellati Timothy J 
Published: September 2016

Abstract
Respiratory infection with Francisella tularensis (Ft) is characterized by a muted, acute host response, followed by sepsis-like syndrome that results in death. Infection with Ft establishes a principally anti-inflammatory environment that subverts host-cell death programs to facilitate pathogen replication. Although the role of cytokines has been explored extensively, the role of eicosanoids in tularemia pathogenesis is not fully understood. Given that lipoxin A4 (LXA4) has anti-inflammatory properties, we investigated whether this lipid mediator affects host responses manifested early during infection. The addition of exogenous LXA4 inhibits PGE2 release by Ft-infected murine monocytes in vitro and diminishes apoptotic cell death. Tularemia pathogenesis was characterized in 5-lipoxygenase-deficient (Alox5−/−) mice that are incapable of generating LXA4. Increased release of proinflammatory cytokines and chemokines, as well as increased apoptosis, was observed in Alox5−/− mice as compared with their wild-type counterparts. Alox5−/− mice also exhibited elevated recruitment of neutrophils during the early phase of infection and increased resistance to lethal challenge. Conversely, administration of exogenous LXA4 to Alox5−/− mice made them more susceptible to infection thus mimicking wild-type animals. Taken together, our results suggest that 5-LO activity is a critical regulator of immunopathology observed during the acute phase of respiratory tularemia, regulating bacterial burden and neutrophil recruitment and production of proinflammatory modulators and increasing morbidity and mortality. These studies identify a detrimental role for the 5-LO–derived lipid mediator LXA4 in Ft-induced immunopathology. Targeting this pathway may have therapeutic benefit as an adjunct to treatment with antibiotics and conventional antimicrobial peptides, which often have limited efficacy against intracellular bacteria.
Caenorhabditis elegansglutamylating enzymes function redundantly in male mating
Authors: Chawla Daniel G. , Shah Ruchi V. , Barth Zachary K. , Lee Jessica D. , Badecker Katherine E. , Naik Anar , Brewster Megan M. , Salmon Timothy P. , Peel Nina 
Published: September 2016

ABSTRACTMicrotubule glutamylation is an important modulator of microtubule function and has been implicated in the regulation of centriole stability, neuronal outgrowth and cilia motility. Glutamylation of the microtubules is catalyzed by a family of tubulin tyrosine ligase-like (TTLL) enzymes. Analysis of individual TTLL enzymes has led to an understanding of their specific functions, but how activities of the TTLL enzymes are coordinated to spatially and temporally regulate glutamylation remains relatively unexplored. We have undertaken an analysis of the glutamylating TTLL enzymes in C. elegans. We find that although all five TTLL enzymes are expressed in the embryo and adult worm, loss of individual enzymes does not perturb microtubule function in embryonic cell divisions. Moreover, normal dye-filling, osmotic avoidance and male mating behavior indicate the presence of functional amphid cilia and male-specific neurons. A ttll-4(tm3310); ttll-11(tm4059); ttll-5(tm3360) triple mutant, however, shows reduced male mating efficiency due to a defect in the response step, suggesting that these three enzymes function redundantly, and that glutamylation is required for proper function of the male-specific neurons.
How great white sharks nourish their embryos to a large size: evidence of lipid histotrophy in lamnoid shark reproduction
Authors: Sato Keiichi , Nakamura Masaru , Tomita Taketeru , Toda Minoru , Miyamoto Kei , Nozu Ryo 
Published: September 2016

ABSTRACT
The great white shark (Carcharodon carcharias) exhibits viviparous and oophagous reproduction. A 4950 mm total length (TL) gravid female accidentally caught by fishermen in the Okinawa Prefecture, Southern Japan carried six embryos (543-624 mm TL, three in each uterus). Both uteri contained copious amounts of yellowish viscous uterine fluid (over 79.2 litres in the left uterus), nutrient eggs and broken egg cases. The embryos had yolk stomachs that had ruptured, the mean volume of which was approximately 197.9 ml. Embryos had about 20 rows of potentially functional teeth in the upper and lower jaws. Periodic acid Schiff (PAS)-positive substances were observed on the surface and in the cytoplasm of the epithelial cells, and large, secretory, OsO4-oxidized lipid droplets of various sizes were distributed on the surface of the villous string epithelium on the uterine wall. Histological examination of the uterine wall showed it to consist of villi, similar to the trophonemata of Dasyatidae rays, suggesting that the large amount of fluid found in the uterus of the white shark was likely required for embryo nutrition. We conclude that: (1) the lipid-rich fluid is secreted from the uterine epithelium only in early gestation before the onset of oophagy, (2) the embryos probably use the abundant uterine fluid and encased nutrient eggs for nutrition at this stage of their development, and (3) the uterine fluid is the major source of embryonic nutrition before oophagy onset. This is the first record of the lipid histotrophy of reproduction among all shark species.
Amontillado is required forDrosophilaSlit processing and for tendon-mediated muscle patterning
Authors: Ordan Elly , Volk Talila 
Published: September 2016

ABSTRACTSlit cleavage into N-terminal and C-terminal polypeptides is essential for restricting the range of Slit activity. Although the Slit cleavage site has been characterized previously and is evolutionally conserved, the identity of the protease that cleaves Slit remains elusive. Our previous analysis indicated that Slit cleavage is essential to immobilize the active Slit-N at the tendon cell surfaces, mediating the arrest of muscle elongation. In an attempt to identify the protease required for Slit cleavage we performed an RNAi-based assay in the ectoderm and followed the process of elongation of the lateral transverse muscles toward tendon cells. The screen led to the identification of the Drosophila homolog of pheromone convertase 2 (PC2), Amontillado (Amon), as an essential protease for Slit cleavage. Further analysis indicated that Slit mobility on SDS polyacrylamide gel electrophoresis (SDS-PAGE) is slightly up-shifted in amon mutants, and its conventional cleavage into the Slit-N and Slit-C polypeptides is attenuated. Consistent with the requirement for amon to promote Slit cleavage and membrane immobilization of Slit-N, the muscle phenotype of amon mutant embryos was rescued by co-expressing a membrane-bound form of full-length Slit lacking the cleavage site and knocked into the slit locus. The identification of a novel protease component essential for Slit processing may represent an additional regulatory step in the Slit signaling pathway.
Rabbit Achilles tendon full transection model – wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery
Authors: Meier Bürgisser Gabriella , Calcagni Maurizio , Bachmann Elias , Fessel Gion , Snedeker Jess G. , Giovanoli Pietro , Buschmann Johanna 
Published: September 2016

ABSTRACT
After tendon rupture repair, two main problems may occur: re-rupture and adhesion formation. Suitable non-murine animal models are needed to study the healing tendon in terms of biomechanical properties and extent of adhesion formation. In this study 24 New Zealand White rabbits received a full transection of the Achilles tendon 2 cm above the calcaneus, sutured with a 4-strand Becker suture. Post-surgical analysis was performed at 3, 6 and 12 weeks. In the 6-week group, animals received a cast either in a 180 deg stretched position during 6 weeks (adhesion provoking immobilization), or were re-casted with a 150 deg position after 3 weeks (adhesion inhibiting immobilization), while in the other groups (3 and 12 weeks) a 180 deg position cast was applied for 3 weeks. Adhesion extent was analyzed by histology and ultrasound. Histopathological scoring was performed according to a method by Stoll et al. (2011), and the main biomechanical properties were assessed. Histopathological scores increased as a function of time, but did not reach values of healthy tendons after 12 weeks (only around 15 out of 20 points). Adhesion provoking immobilization led to an adhesion extent of 82.7±9.7%, while adhesion inhibiting immobilization led to 31.9±9.8% after 6 weeks. Biomechanical properties increased over time, however, they did not reach full strength nor elastic modulus at 12 weeks post-operation. Furthermore, the rabbit Achilles tendon model can be modulated in terms of adhesion formation to the surrounding tissue. It clearly shows the different healing stages in terms of histopathology and offers a suitable model regarding biomechanics because it exhibits similar biomechanics as the human flexor tendons of the hand.
Cancer metabolism at a glance
Authors: Vazquez Alexei , Kamphorst Jurre J. , Markert Elke K. , Schug Zachary T. , Tardito Saverio , Gottlieb Eyal 
Published: September 2016

ABSTRACT
A defining hallmark of cancer is uncontrolled cell proliferation. This is initiated once cells have accumulated alterations in signaling pathways that control metabolism and proliferation, wherein the metabolic alterations provide the energetic and anabolic demands of enhanced cell proliferation. How these metabolic requirements are satisfied depends, in part, on the tumor microenvironment, which determines the availability of nutrients and oxygen. In this Cell Science at a Glance paper and the accompanying poster, we summarize our current understanding of cancer metabolism, emphasizing pathways of nutrient utilization and metabolism that either appear or have been proven essential for cancer cells. We also review how this knowledge has contributed to the development of anticancer therapies that target cancer metabolism.
TOURISM AND INTANGIBLE CULTURAL HERITAGE: KARELIAN-FINNISH EPIC POEM «KALEVALA» AS ATTRACTOR IN INFORMATION FIELD OF DESTINATION
Authors: Колесников Николай , Kolesnikov Nikolay , Колесникова Наталья , Kolesnikova Natalya , Плотникова Виктория , Plotnikova Viktoriya 
Published: September 2016

The article investigates the forms and principles of involving intangible cultural heritage in tourism development thought the example of the Republic of Karelia. Various forms of intangible cultural heritage are presented according to the UNESCO Convention for the Safeguarding of the Intangible Cultural Heritage 2003. Forms of using of intangible cultural heritage in the framework of the various types of tourism organization are explored through the certain examples. The authors consider 4 conditions of legend’s involvement in the service process in the territory of tourism destination according to the «Scheherazade strategy» by Christian Salmon: (1) submission of natural and cultural heritage through the history / legend; (2) certain time of story / legend providing; (3)«message» of tourism destinations; (4) creation of information field on the Internet. The authors specify the 3rd point and formulated it as «formation of cultural anchor» that should «tie» specific local legend to places and objects located in the territory.
The intangible cultural heritage involvement in the process of tourist services organization is considered by the example of the Karelian-Finnish epic poem «Kalevala», consisting of 50 runes (the Republic of Karelia). Experience of the «Kalevala» epic poem involvement in tourism is considered: tourism routes development, creating and adaptation of legends, development of event and adventure tourism. The possibility of implementing the updated «Scheherazade strategy» by the example of the epic poem «Kalevala» is investigated. As the research result, the authors propose an annual roleplaying game «Kalevala. Amazing adventure» as the new actual form of promotion «Kalevala» epic poem. This project was developed by students and lectures of Tourism Department of Petrozavodsk State University and included to the Small Deal Strategy of Kalevala region of the Republic of Karelia.
This article was prepared as part of the scientific activities of the Strategic Development Program of Petrozavodsk State University for 2012–2016.
BELMOND HOTEL DAS CATARATAS: investigação das transformações ocorridas a partir da implantação do SGA NBR ISO 14001
Authors: Jacomelli Eloise , Rodrigues Zaidyani Gabine , Hack Eduardo 
Published: September 2016

O artigo trata de um resgate do processo de implantação do SGA - iniciado com a empresa Tropical Hotels & Resorts (de 2000 até 2007), até os investimentos e resultados alcançados pelo grupo Orient- Express Brasil S/A (de 2007 até meados de 2014). De forma geral, objetiva-se investigar as transformações ocorridas dos elementos indicativos e práticas a partir da implantação do SGA. Especi camente, descrever o processo de implantação destes sistemas dos dois grupos hoteleiros, levantar os indicadores e práticas de Gestão Ambiental, e veri car a variabilidade estatística (desvio padrão) dos elementos indicativos das práticas de gestão ambiental (consumo esperado versus consumo real). Os métodos caracterizam-se com o uso de técnicas qualitativas e quantitativas, com bases exploratórias e descritivas, o instrumento utilizado fora à entrevista semiestruturada, com técnica de análise de conteúdo, aplicado com a Coordenadora de Aprendizagem e Desenvolvimento, com o Gerente de Manutenção e com o Supervisor de Tecnologia da Informação do hotel sobre os indicadores ambientais aplicados na empresa. Os resultados alcançados demonstram que a organização continua trabalhando de forma adequada o SGA, sobretudo, busca soluções que reduzam o consumo de água, energia, gás natural, dentre outros indicadores. Ao nal demonstram-se propostas de melhora ao empreendimento.Palavras-chave: ISO 14001; Gestão Ambiental; Sistema de Gestão Ambiental; Indicadores de Gestão Ambiental.
HABILIDADES NA CAPTAÇÃO E NEGOCIAÇÃO DE PROJETOS TURÍSTICOS: A percepção dos consultores
Authors: Novaes Marlene Huebes , Pernanchini Alan Ceratti 
Published: September 2016

O presente artigo busca elencar, através de uma pesquisa realizada com pro ssionais da área de consultoria, as habilidades utilizadas pelos mesmos durante as etapas de captação e negociação de projetos turísticos, comparando com as habilidades publicadas por autores especialistas da área. A metodologia aplicada foi pesquisa do tipo bibliográ ca, exploratória, quantitativa com análise multivariada de clusters. Através deste método formaram-se três grupos, compostos pelas respostas dos entrevistados, buscando através de uma distância denominada euclidiana a dissimilaridade desses grupos. Na análise dos clusters, foi possível perceber as diferenças e semelhanças geradas pelos grupos, como a maneira que os consultores da área classi cam o processo de desenvolvimento de suas habilidades, durante o processo de consultoria. Grande parte dos entrevistados destacou como a principal in uência para o desenvolvimento de novas habilidades as pesquisas, artigos, publicações sobre o comportamento do consumidor, mercados e tendências, leitura de livros relacionados à vendas, negociações e elaboração de projetos. O que permite inferir que o desenvolvimento de habilidades é essencial no decorrer da carreira de um consultor. Ressalta-se a necessidade dos consultores buscarem o desenvolvimento constante para agregar valor aos seus serviços.Palavras-chave: Turismo. Hotelaria. Consultoria. Habilidades.
The Feldstein-Horioka hypothesis revisited
Authors: Dzhumashev Ratbek , Cooray Arusha 
Published: September 2016

Abstract
Feldstein-Horioka hypothesis states that if there is perfect capital mobility, low correlation between domestic investment and savings should be observed. However, empirical analysis failed to confirm the hypothesis. This study attempts to shed a new light on international capital mobility by incorporating the effect of the fiscal balance and the financial balance. Specifically, for a panel of 161 countries over the 1990–2013 period, the extended model is tested in comparison with the existing models of capital mobility. At the aggregate level, strong support is found for the extended model; while, at the region disaggregated level, compared to the existing models the hypothesis of capital mobility holds for a larger number of regions. Our model and estimates are additionally extended to account for the fact that the above mentioned relationships, are conditional on the country’s level of financial development.
Synthesis and characterization of polyHIPE composites containing halloysite nanotubes
Authors: Hande Mert Hatice , Şen Sinan 
Published: September 2016

Abstract
High internal phase emulsion templated-polymer (polyHIPE) composites were prepared from spirulina modified halloysite (HL) nanotube containing styrene/divinylbenzene based water-in-oil type concentrated emulsions. In order to obtain a stable emulsion for neat polyHIPE’s synthesis, at least 5 vol% Span-80 as a non-ionic surfactant, with respect to organic phase was needed. For syntheses of polyHIPE composite structures, this amount was decreased to 2 vol%, even in presence of 0.25 wt% modified nanotube with respect to the organic phase. All the polyHIPE composites exhibited open pore structures with pore interconnections together with partially or completely closed pores. The composite having 0.25 wt% modified nanotube and 2 vol% surfactant was found to have about 260% higher dye adsorption capacity and the highest onset degradation temperature in comparison with neat polyHIPE.