bluuebunny/crossref_metadata_2025_split huggingface dataset:
NES1/KLK10 gene represses proliferation, enhances apoptosis and down-regulates glucose metabolism of PC3 prostate cancer cells
Authors: Hu Jiajia , Lei Hu , Fei Xiaochun , Liang Sheng , Xu Hanzhang , Qin Dongjun , Wang Yue , Wu Yingli , Li Biao 
Published: November 2015

AbstractThe normal epithelial cell-specific-1 (NES1) gene, also named as KLK10, is recognised as a novel putative tumour suppressor in breast cancer, but few studies have focused on the function of KLK10 in human prostate cancer. Our study confirms that the expression of KLK10 in prostate cancer tissue and cell lines (PC3, DU145 and LNCaP clone FGC) is low. Given that the androgen-independent growth characteristic of the PC3 cell line is more similar to clinical castration-resistant prostate cancer, we studied the role of KLK10 in PC3. In vitro and in vivo assays showed that over-expressing KLK10 in PC3 could decelerate tumour proliferation, which was accompanied with an increase in apoptosis and suppression of glucose metabolism. The related proteins, such as Bcl-2 and HK-2, were down-regulated subsequently. Furthermore, by up-regulating Bcl-2 or HK-2 respectively in the PC3-KLK10 cell line, we observed a subsequent increase of cell proliferation and a synchronous up-regulation of HK-2 and Bcl-2. Besides, KLK10 expression was also increased by Bcl-2 and HK-2, which suggests that there is a negative feedback loop between KLK10 and Bcl-2/HK-2. Thus, our results demonstrated that KLK10 may function as a tumour suppressor by repressing proliferation, enhancing apoptosis and decreasing glucose metabolism in PC3 cells.
In Vivo Linking of Membrane Lipids and the Anion Transporter Band 3 with Thiourea-modified Amphiphilic Lipid Probes
Authors: Moriyama Akihiro , Katagiri Naohiro , Nishimura Shinichi , Takahashi Nobuaki , Kakeya Hideaki 
Published: November 2015

AbstractMembrane proteins interact with membrane lipids for their structural stability and proper function. However, lipid–protein interactions are poorly understood at a molecular level especially in the live cell membrane, due to current limitations in methodology. Here, we report that amphiphilic lipid probes can be used to link membrane lipids and membrane proteins in vivo. Cholesterol and a phospholipid were both conjugated to a fluorescent tag through a linker containing thiourea. In the erythrocyte, the cholesterol probe fluorescently tagged the anion transporter band 3 via thiourea. Tagging by the cholesterol probe, but not by the phospholipid probe, was competitive with an anion transporter inhibitor, implying the presence of a specific binding pocket for cholesterol in this ~100 kDa protein. This method could prove an effective strategy for analyzing lipid–protein interactions in vivo in the live cell membrane.
Short-term hyperoxia does not exert immunologic effects during experimental murine and human endotoxemia
Authors: Kiers Dorien , Gerretsen Jelle , Janssen Emmy , John Aaron , Groeneveld R. , van der Hoeven Johannes G. , Scheffer Gert-Jan , Pickkers Peter , Kox Matthijs 
Published: November 2015

AbstractOxygen therapy to maintain tissue oxygenation is one of the cornerstones of critical care. Therefore, hyperoxia is often encountered in critically ill patients. Epidemiologic studies have demonstrated that hyperoxia may affect outcome, although mechanisms are unclear. Immunologic effects might be involved, as hyperoxia was shown to attenuate inflammation and organ damage in preclinical models. However, it remains unclear whether these observations can be ascribed to direct immunosuppressive effects of hyperoxia or to preserved tissue oxygenation. In contrast to these putative anti-inflammatory effects, hyperoxia may elicit an inflammatory response and organ damage in itself, known as oxygen toxicity. Here, we demonstrate that, in the absence of systemic inflammation, short-term hyperoxia (100% O2 for 2.5 hours in mice and 3.5 hours in humans) does not result in increased levels of inflammatory cytokines in both mice and healthy volunteers. Furthermore, we show that, compared with room air, hyperoxia does not affect the systemic inflammatory response elicited by administration of bacterial endotoxin in mice and man. Finally, neutrophil phagocytosis and ROS generation are unaffected by short-term hyperoxia. Our results indicate that hyperoxia does not exert direct anti-inflammatory effects and temper expectations of using it as an immunomodulatory treatment strategy.
Environmental Surveillance of Genogroup I and II Noroviruses in Shandong Province, China in 2013
Authors: Tao Zexin , Xu Minglei , Lin Xiaojuan , Wang Haiyan , Song Lizhi , Wang Suting , Zhou Nan , Zhang Dongfeng , Xu Aiqiang 
Published: November 2015

AbstractNoroviruses are the most common cause of epidemic gastroenteritis. However, the case-based surveillance is limited in China. In this study, we analyzed the results of environmental surveillance conducted in two cities of Shandong Province, China from January to December in 2013. Twenty-four sewage samples were collected and concentrated via membrane absorption/elution method. After reverse transcription-PCR, cloning and sequencing on ORF2 region, norovirus nucleic acid was detected in all 24 sewage samples. A total of 403 norovirus sequences of 16 genotypes were detected, among which GII.3 (22.6%), GI.2 (17.1%), GI.5 (13.4%), GI.3 (11.9%), GII.4 (7.7%) and GII.6 (6.7%) were the 6 most common genotypes. Phylogenetic analysis revealed multiple lineages within most common genotypes, especially in GI.3, whereas all GII.4 sequences belonged to Sydney 2012 strain. Recombination events were observed in 5 GI and 4 GII sequences within or near the ORF1/ORF2 overlap. This is the first report on systematic environmental surveillance on norovirus in China. The data presented here reveal co-circulation and high genetic diversity of multiple norovirus genotypes in the two cities and suggest continued environmental surveillance can provide valuable information on norovirus circulation in the population.
Conjunctival fibrosis and the innate barriers to Chlamydia trachomatis intracellular infection: a genome wide association study
Authors: Roberts Chrissy h. , Franklin Christopher S. , Makalo Pateh , Joof Hassan , Sarr Isatou , Mahdi Olaimatu S. , Sillah Ansumana , Bah Momodou , Payne Felicity , Jeffreys Anna E. , Bottomley William , Natividad Angels , Molina-Gonzalez Sandra , Burr Sarah E. , Preston Mark , Kwiatkowski Dominic , Rockett Kirk A. , Clark Taane G. , Burton Matthew J. , Mabey David C. W. , Bailey Robin , Barroso Inês , Holland Martin J. 
Published: November 2015

AbstractChlamydia trachomatis causes both trachoma and sexually transmitted infections. These diseases have similar pathology and potentially similar genetic predisposing factors. We aimed to identify polymorphisms and pathways associated with pathological sequelae of ocular Chlamydia trachomatis infections in The Gambia. We report a discovery phase genome-wide association study (GWAS) of scarring trachoma (1090 cases, 1531 controls) that identified 27 SNPs with strong, but not genome-wide significant, association with disease (5 × 10−6 > P > 5 × 10−8). The most strongly associated SNP (rs111513399, P = 5.38 × 10−7) fell within a gene (PREX2) with homology to factors known to facilitate chlamydial entry to the host cell. Pathway analysis of GWAS data was significantly enriched for mitotic cell cycle processes (P = 0.001), the immune response (P = 0.00001) and for multiple cell surface receptor signalling pathways. New analyses of published transcriptome data sets from Gambia, Tanzania and Ethiopia also revealed that the same cell cycle and immune response pathways were enriched at the transcriptional level in various disease states. Although unconfirmed, the data suggest that genetic associations with chlamydial scarring disease may be focussed on processes relating to the immune response, the host cell cycle and cell surface receptor signalling.
Unsuppressed lipolysis in adipocytes is linked with enhanced gluconeogenesis and altered bile acid physiology in InsrP1195L/+ mice fed high-fat-diet
Authors: Lee Eun Young , Sakurai Kenichi , Zhang Xilin , Toda Chitoku , Tanaka Tomoaki , Jiang Meizi , Shirasawa Takuji , Tachibana Kaori , Yokote Koutaro , Vidal-Puig Antonio , Minokoshi Yasuhiko , Miki Takashi 
Published: November 2015

AbstractHigh-fat diet (HFD) triggers insulin resistance and diabetes mellitus, but their link remains unclear. Characterization of overt hyperglycemia in insulin receptor mutant (InsrP1195L/+) mice exposed to HFD (InsrP1195L/+/HFD mice) revealed increased glucose-6-phosphatase (G6pc) expression in liver and increased gluconeogenesis from glycerol. Lipolysis in white adipose tissues (WAT) and lipolysis-induced blood glucose rise were increased in InsrP1195L/+/HFD mice, while wild-type WAT transplantation ameliorated the hyperglycemia and the increased G6pc expression. We found that the expressions of genes involved in bile acid (BA) metabolism were altered in InsrP1195L/+/HFD liver. Among these, the expression of Cyp7a1, a BA synthesis enzyme, was insulin-dependent and was markedly decreased in InsrP1195L/+/HFD liver. Reduced Cyp7a1 expression in InsrP1195L/+/HFD liver was rescued by WAT transplantation and the expression of Cyp7a1 was suppressed by glycerol administration in wild-type liver. These findings suggest that unsuppressed lipolysis in adipocytes elicited by HFD feeding is linked with enhanced gluconeogenesis from glycerol and with alterations in BA physiology in InsrP1195L/+/HFD liver.
The role of Drosophila mismatch repair in suppressing recombination between diverged sequences
Authors: Do Anthony T. , LaRocque Jeannine R. 
Published: November 2015

AbstractDNA double-strand breaks (DSBs) must be accurately repaired to maintain genomic integrity. DSBs can be repaired by homologous recombination (HR), which uses an identical sequence as a template to restore the genetic information lost at the break. Suppression of recombination between diverged sequences is essential to the repair of DSBs without aberrant and potentially mutagenic recombination between non-identical sequences, such as Alu repeats in the human genome. The mismatch repair (MMR) machinery has been found to suppress recombination between diverged sequences in murine cells. To test if this phenomenon is conserved in whole organisms, two DSB repair systems were utilized in Drosophila melanogaster. The DR-white and DR-white.mu assays provide a method of measuring DSB repair outcomes between identical and diverged sequences respectively. msh6–/– flies, deficient in MMR, were not capable of suppressing recombination between sequences with 1.4% divergence and the average gene conversion tract length did not differ between msh6–/+ and msh6–/–flies. These findings suggest that MMR has an early role in suppressing recombination between diverged sequences that is conserved in Drosophila.
Near‐threshold all‐digital PLL with dynamic voltage scaling power management
Authors: Chang C.‐W. , Chang K.‐Y. , Chu Y.‐H. , Jou S.‐J. 
Published: November 2015

A near‐threshold all‐digital phase‐locked loop (ADPLL) with a power management unit (PMU) is presented to make the proposed ADPLL work reliably across variations and power consumption as well is reduced. When operated under near‐threshold condition from 0.52 to 0.58 V VDD, the gated digitally controlled oscillator frequency range is from 90.8 to 245.7 MHz. When the ADPLL is operated at 0.52 V VDD, a lock‐in time of 9.5 μs at 100 MHz output clock frequency is measured with an rms period jitter of 0.17% UI. With the PMU, the ADPLL power reduction at 130 MHz output frequency is 39% and the buck converter power consumption is nearly 30 μW. Consequently, the proposed ADPLL with PMU is suitable to event‐driven or low‐voltage applications.
Feasibility and Evaluation of Probabilistic On-site Surveys for Estimating Angler Use and Catch in High Mountain Lakes
Authors: McCormick Joshua L. 
Published: November 2015

Abstract
Estimating angling effort and catch at high mountain lakes can be difficult due the abundance, remoteness, and diffuse nature of angling effort that typify high mountain lake fisheries. In this study, a simulation was used to evaluate the accuracy of catch and effort estimates derived using on-site access–access and roving–access creel surveys at a complex of 35 high mountain lakes. Five levels of angling effort and catch at the 35 lakes were simulated, and effort varied from 3,278 to 68,741 h and catch varied from 1,737 to 50,525 fish over the duration of the season. Access–access creel surveys had an average of 32% relative error and roving–access surveys had an average of 17% relative error in estimates of aggregate (i.e., at all 35 lakes) angling effort and catch when one creel surveyor was used. Estimates were relatively robust to temporal and spatial changes in patterns of effort and catch rate over the duration of the season. Relative error was inversely related to the amount of angling effort, catch, and creel surveyors. Roving–access surveys outperformed access–access surveys at all levels of sampling effort. Bias and relative error of estimates of angling effort and catch were greater at the individual-lake scale than when estimates were derived for all 35 lakes. Results of this study suggest that on-site surveys can provide relatively accurate estimates of angling effort and catch at as many as 35 lakes with minimal sampling effort.
Received March 16, 2015; accepted August 14, 2015
Report on the EURAMET key comparison EURAMET.AUV.V-K3
Authors: Bartoli C , Prasil M , Andersson H , Hof C , Schiavi A , Kolasa J , Medina N , Bruns T , Gazioch S , Licht T R , Hamalainen J 
Published: November 2015

This report presents the result of the first EURAMET comparison in the area of 'low frequency vibration', in this case implying sinusoidal acceleration between DC and 200 Hz

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCAUV, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Scallops caught in the headlights: swimming escape behaviour of the Atlantic sea scallop (Placopecten magellanicus) reduced by artificial light
Authors: Siemann Liese A. , Parkins Christopher J. , Smolowitz Ronald J. 
Published: November 2015

AbstractQuantifying the distribution and abundance of the Atlantic sea scallop (Placopecten magellanicus) is a fishery management priority, and stock assessments increasingly rely on video surveys. Interpreting the results of these surveys requires understanding the inherent biases introduced as a result of target animal behaviour. Our study investigated the effect of artificial lights on the behaviour of Atlantic sea scallops during a video survey using a towed benthic sled. Swimming and stationary scallops were counted in survey videos using event logging software. In addition, the locations, orientations, and swimming directions of the scallops were noted in a subset of the videos. The proportion of scallops that swam when an artificial light was turned on was significantly smaller than the proportion that swam when the light was off. Further analysis using a logistic model showed that only light state (off or on) predicted the likelihood of scallop swimming responses. Possible reasons for this unexpected behaviour are discussed, with a focus on the scallop visual system.
Automatic segmentation ofDrosophilaneural compartments using GAL4 expression data reveals novel visual pathways
Authors: Panser K , Tirian L , Schulze F , Villalba S , Jefferis GSXE , Bühler Katja , Straw AD 
Published: November 2015

AbstractWe made use of two recent, large-scaleDrosophilaGAL4 libraries and associated confocal imaging datasets to automatically segment large brain regions into smaller putative functional units such as neuropils and fiber tracts. The method we developed is based on the hypothesis that molecular identity can be used to assign individual voxels to biologically meaningful regions. Our results (available athttps://strawlab.org/braincode) are consistent with this hypothesis because regions with well-known anatomy, namely the antennal lobes and central complex, were automatically segmented into familiar compartments. We then applied the algorithm to the central brain regions receiving input from the optic lobes. Based on the automated segmentation and manual validation, we can identify and provide promising driver lines for 10 previously identified and 14 novel types of visual projection neurons and their associated optic glomeruli. The same strategy can be used in other brain regions and likely other species, including vertebrates.
Revolutionising Public Health Reference Microbiology using Whole Genome Sequencing:Salmonellaas an exemplar
Authors: Ashton Philip , Nair Satheesh , Peters Tansy , Tewolde Rediat , Day Martin , Doumith Michel , Green Jonathan , Jenkins Claire , Underwood Anthony , Arnold Catherine , de Pinna Elizabeth , Dallman Tim , Grant Kathie 
Published: November 2015

AbstractAdvances in whole genome sequencing (WGS) platforms and DNA library preparation have led to the development of methods for high throughput sequencing of bacterial genomes at a relatively low cost (Loman et al. 2012; Medini et al. 2008). WGS offers unprecedented resolution for determining degrees of relatedness between strains of bacterial pathogens and has proven a powerful tool for microbial population studies and epidemiological investigations (Harris et al. 2010; Lienau et al. 2011; Holt et al. 2009; Ashton, Peters, et al. 2015). The potential utility of WGS to public health microbiology has been highlighted previously (Köser et al. 2012; Kwong et al. 2013; Reuter et al. 2013; Joensen et al. 2014; Nair et al. 2014; Bakker et al. 2014; D’Auria et al. 2014). Here we report, for the first time, the routine use of WGS as the primary test for identification, surveillance and outbreak investigation by a national reference laboratory. We present data on how this has revolutionised public health microbiology for one of the most common bacterial pathogens in the United Kingdom, theSalmonellae.DATA SUMMARY1. PHE Salmonella sequencing data is deposited in the Sequence Read Archive in BioProject PRJNA248792.IMPACT STATEMENTThe first human genome cost around $3 billion, and took around 10 years to complete. Advances in DNA sequencing technology (also referred to as whole genome sequencing (WGS)) allow the same feat to be accomplished today for less than $10000 and less than 2 weeks. This remarkable improvement in technology has also led to a step change in microbiology, increasing our understanding of the evolution of major human pathogens such asYersinia pestis, SalmonellaTyphi andMycobacterium tuberculosis.While these kinds of academic studies provide unparalleled context for public health action, until now, this approach has not been routinely employed at the frontline. At Public Health England, WGS has been implemented for routine public health identification, characterisation and typing of an important human pathogen,Salmonella,replacing methods that have changed little over the last 100 years. Analysis of WGS data has identified outbreaks that were previously undetectable and been used to infer rare antimicrobial resistance patterns. This paper will serve as a notification to the community of the methods PHE are using, and will be of great use to other public health labs considering switching to WGS.
The Role of Visual Attention in the Managerial Judgment of Balanced‐Scorecard Performance Evaluation: Insights from Using an Eye‐Tracking Device
Authors: CHEN YASHENG , JERMIAS JOHNNY , PANGGABEAN TOTA 
Published: November 2015

ABSTRACTThis paper investigates the role of visual attention in managerial judgments during balanced‐scorecard performance evaluations. Using the Locarna eye tracker to establish the amount of time managers spent focused on visual cues, we found that managers who look more at strategically linked performance measures are more likely to make decisions consistent with the achievement of their subordinates’ strategic objectives. When aware of strategy, managers focused more on strategically linked performance measures than on nonlinked measures. The presentation format of the strategy information did not significantly affect this focus. Our findings indicate that awareness of strategically linked performance measures, but not their presentation, appears to be important in helping managers to make better decisions. This study contributes to the management accounting literature by generating useful insights into the impact of visual attention on judgments and decision‐making processes.
Beneficial Influence of Short‐Term Germination on Decreasing Allergenicity of Peanut Proteins
Authors: Li Yingchao , Sun Xiulan , Ma Zhezhe , Cui Yan , Du Chao , Xia Xiuhua , Qian He 
Published: November 2015

AbstractMost allergenic storage proteins in peanuts are degraded during seed germination. By altering this natural physiological process, it might be possible to reduce peanut protein allergenicity. However, little is known about the change in allergenic proteins and their corresponding immunocreactivity, and the effects of major environmental conditions on their allergenicity during germination. In this study, the influence of different germination conditions (temperature and light) on the degradation of Ara h1 and allergenicity changes of peanut seeds was evaluated by ELISA and Western blotting. The results showed that the 40‐ and 65‐kDa proteins in peanut seeds degraded rapidly during the time course, beginning at 60 (at 25 °C) and 108 h (at 20 °C), and the corresponding immunocreactivity of Ara h1 decreased approximately one‐third after 5 to 7 d of germination. Compared with the cotyledons, the embryonic axes had a higher proportion of Ara h1, which was then degraded relatively faster during germination, resulting in a significant reduction in its allergenicity. Although a higher temperature improved the seed germination rate, it affected sprout quality (as did light); therefore, 25 °C and dark surroundings were suitable conditions under which peanut sprouts were processed; neither factor significantly affected the allergenicity of Ara h1. These results provided a theoretical basis for studies using biological methods to reduce peanut allergenicity.
The marine bacteria Shewanella frigidimarina NCIMB400 upregulates the type VI secretion system during early biofilm formation
Authors: Linares Denis , Jean Natacha , Van Overtvelt Perrine , Ouidir Tassadit , Hardouin Julie , Blache Yves , Molmeret Maëlle 
Published: November 2015

SummaryShewanella sp. are facultative anaerobic Gram‐negative bacteria, extensively studied for their electron transfer ability. Shewanella frigidimarina has been detected and isolated from marine environments, and in particular, from biofilms. However, its ability to adhere to surfaces and form a biofilm is poorly understood. In this study, we show that the ability to adhere and to form a biofilm of S. frigidimarina NCIMB400 is significantly higher than that of Shewanella oneidensis in our conditions. We also show that this strain forms a biofilm in artificial seawater, whereas in Luria–Bertani, this capacity is reduced. To identify proteins involved in early biofilm formation, a proteomic analysis of sessile versus planktonic membrane‐enriched fractions allowed the identification of several components of the same type VI secretion system gene cluster: putative Hcp1 and ImpB proteins as well as a forkhead‐associated domain‐containing protein. The upregulation of Hcp1 a marker of active translocation has been confirmed using quantitative reverse transcription polymerase chain reaction. Our data demonstrated the presence of a single and complete type VI secretion system in S. frigidimarina NCIMB400 genome, upregulated in sessile compared with planktonic conditions. The fact that three proteins including the secreted protein Hcp1 have been identified may suggest that this type VI secretion system is functional.