bluuebunny/crossref_metadata_2025_split huggingface dataset:
SmartScope2: Simultaneous Imaging and Reconstruction of Neuronal Morphology
Authors: Long Brian , Zhou Zhi , Cetin Ali , Ting Jonathan , Gwinn Ryder , Tasic Bosiljka , Daigle Tanya , Lein Ed , Zeng Hongkui , Saggau Peter , Hawrylycz Michael , Peng Hanchuan 
Published: March 2017

AbstractQuantitative analysis of neuronal morphology is critical in cell type classification and for deciphering how structure gives rise to function in the brain. Most current approaches to imaging and tracing neuronal 3D morphology are data intensive. We introduce SmartScope2, the first open source, automated neuron reconstruction machine integrating online image analysis with automated multiphoton imaging. SmartScope2 takes advantage of a neuron’s sparse morphology to improve imaging speed and reduce image data stored, transferred and analyzed. We show that SmartScope2 is able to produce the complex 3D morphology of human and mouse cortical neurons with six-fold reduction in image data requirements and three times the imaging speed compared to conventional methods.
Normal spindle positioning in the absence of EBPs and dynein plus-end tracking inC. elegans
Authors: Schmidt Ruben , Akhmanova Anna , van den Heuvel Sander 
Published: March 2017

AbstractThe position of the mitotic spindle is tightly controlled in animal cells, as it determines the plane and orientation of cell division. Interactions between cytoplasmic dynein at the cortex and astral microtubules generate pulling forces that position the spindle. In yeast, dynein is actively delivered to the cortex through microtubule plus-end tracking complexes. In animal cells, an evolutionarily conserved Gα-GPR-1/2Pins/LGN–LIN-5NuMAcortical complex interacts with dynein and is required to generate pulling forces, but the mechanism of dynein recruitment to the cortex is unclear. Using CRISPR/Cas9-assisted recombineering, we fluorescently labeled endogenous DHC-1 dynein inC. elegans.We observed strong dynein plus-end tracking, which depended on the end-binding protein EBP-2. Complete removal of the EBP family abolished dynein plus-end tracking but not LIN-5-dependent cortical localization. Theebp-1/2/3deletion mutant, which was viable and fertile, showed increased cortical microtubule retention; however, pulling forces and spindle positioning were normal. These data indicate that dynein recruited from the cytoplasm creates robust pulling forces.
Investigating the genetic regulation of the expression of 63 lipid metabolism genes in the pig skeletal muscle
Authors: González-Prendes Rayner , Quintanilla Raquel , Amills Marcel 
Published: March 2017

AbstractDespite their potential involvement in the determination of fatness phenotypes, a comprehensive and systematic view about the genetic regulation of lipid metabolism genes is still lacking in pigs. Herewith, we have used a dataset of 104 pigs, with available genotypes for 62,163 single nucleotide polymorphisms and microarray gene expression measurements in thegluteus mediusmuscle, to investigate the genetic regulation of 63 genes with crucial roles in the uptake, transport, synthesis and catabolism of lipids. By performing an eQTL scan with the GEMMA software, we have detected 12 cis- and 18 trans-eQTL modulating the expression of 19 loci. Genes regulated by eQTL had a variety of functions such as the β-oxidation of fatty acids, lipid biosynthesis and lipolysis, fatty acid activation and desaturation, lipoprotein uptake, apolipoprotein assembly and cholesterol trafficking. These data provide a first picture about the genetic regulation of loci involved in porcine lipid metabolism.
Assessing dynamic functional connectivity in heterogeneous samples
Authors: Lehmann B. C. L. , White S. R. , Henson R. N. , Cam-CAN  , Geerligs L. 
Published: March 2017

AbstractSeveral methods have been developed to measure dynamic functional connectivity (dFC) in fMRI data. These methods are often based on a sliding-window analysis, which aims to capture how the brain’s functional organization varies over the course of a scan. The aim of many studies is to compare dFC across groups, such as younger versus older people. However, spurious group differences in measured dFC may be caused by other sources of heterogeneity between people. For example, the shape of the haemodynamic response function (HRF) and levels of measurement noise have been found to vary with age. We use a generic simulation framework for fMRI data to investigate the effect of such heterogeneity on estimates of dFC. Our findings show that, despite no differences in true dFC, individual differences in measured dFC can result from other (non-dynamic) features of the data, such as differences in neural autocorrelation, HRF shape, connectivity strength and measurement noise. We also find that common dFC methods such as k-means and multilayer modularity approaches can detect spurious group differences in dynamic connectivity due to inappropriate setting of their hyperparameters. fMRI studies therefore need to consider alternative sources of heterogeneity across individuals before concluding differences in dFC.
Prior expectations induce pre-stimulus sensory templates
Authors: Kok Peter , Mostert Pim , de Lange Floris P. 
Published: March 2017

AbstractPerception can be described as a process of inference, integrating bottom-up sensory inputs and top-down expectations. However, it is unclear how this process is neurally implemented. It has been proposed that expectations lead to pre-stimulus baseline increases in sensory neurons tuned to the expected stimulus, which in turn affects the processing of subsequent stimuli. Recent fMRI studies have revealed stimulus-specific patterns of activation in sensory cortex as a result of expectation, but this method lacks the temporal resolution necessary to distinguish pre- from post-stimulus processes. Here, we combined human MEG with multivariate decoding techniques to probe the representational content of neural signals in a time-resolved manner. We observed a representation of expected stimuli in the neural signal well before they were presented, demonstrating that expectations indeed induce a pre-activation of stimulus templates. These results suggest a mechanism for how predictive perception can be neurally implemented.
Reversing the Thyroid Hormone Mediated Repression of a HSV-1 Promoter through Computationally Guided Site Directed Mutagenesis
Authors: Figliozzi Robert W , Chen Feng , Hsia Shaochung V 
Published: March 2017

AbstractThyroid hormones (TH or T3) and their DNA binding nuclear receptors (TRs), direct transcriptional regulation in different ways depending on the host cell environment and specific promoter characteristics of TH sensitive genes. This study sought to elucidate the impact on repression of nucleotide sequence/orientation of TR binding sites, TR elements, (TREs) within TH sensitive promoters. Computational analysis of the HSV-1 thymidine kinase (TK) gene TRE bound by TR and RXR revealed a single TRE point mutation sufficient to reverse the TRE orientation. In vitro experiments corroborated that the TRE point mutation exhibited distinct impacts on promoter activity, sufficient to reverse the TH dependent negative regulation in neuro-endocrine differentiated cells. EMSA and ChIP experiments suggest that this point mutation altered the promoter’s regulatory mechanism through discrete changes in transcription factor Sp1 and TR occupancy and altered enrichment of repressive chromatin, histone-3-lysine-9-trimethyl (H3K9Me3). Incites relating to this negative TRE (nTRE) mechanism impacts the understanding of other nTREs and TRE mutations associated with TH and herpes diseases.
Crystallization and X-ray analysis of the extracellular adhesion domain ofHelicobacter pyloriadhesin A: the significance of the cation composition in the crystallization precipitant
Authors: Guo Ling , Zhang Jinyong , Cui Liwei , Liu Dong , Ma Bo , Wang Shufeng , Li Haibo , Wu Yuzhang , Liu Wei 
Published: March 2017

Adherence to host cells is a crucial step in the process of bacterial infection, which is usually mediated by a number of outer membrane proteins identified as adhesins.Helicobacter pyloriadhesin A (HpaA) is a member of the adhesin family that mediates the adherence ofHelicobacter pylorito gastric epithelial cells, and consequently assists the bacteria in becoming a life-long colonizer of the human stomach. In this study, two constructs were made for the production of truncated HpaA proteins comprising residues 31–260 and 53–260, respectively. The products of both constructs were crystallized, but only the protein from the shorter construct (residues 53–260) formed crystals that were capable of diffraction. In the subsequent optimization trials, crystals in different forms were unexpectedly obtained by using lithium sulfate and ammonium sulfate as the precipitant. An X-ray data set was collected to 1.95 Å resolution on beamline BL18U1 at SSRF using a crystal grown with 1.92 Mlithium sulfate, which belonged to space groupP65with unit-cell parametersa=b= 95.42,c = 54.72 Å, γ = 120°, while another crystal grown with 1.9 Mammonium sulfate diffracted to 2.60 Å resolution and the collected data set was indexed in space groupP21212, with unit-cell parametersa= 121.01,b= 190.56,c= 106.31 Å. The collection of diffraction data has established a solid basis for structure determination.
Benchmarking of best practices: an overview of the academic literature
Authors: Castro Vinicius Ferreira de , Frazzon Enzo Morosini 
Published: March 2017

Purpose
The purpose of this paper is to overview the academic literature on benchmarking of best practices tracking the most important articles and understanding the similarity between studies.

Design/methodology/approach
The most relevant articles were identified using techniques such as the PageRank algorithm and co-citation maps. An analysis of the most relevant articles presents the main concepts, methods and updated discussions on this research field.

Findings
The analysis of academic output substantiates the increase in the production of articles on this research topic. There were identified two main clusters of co-cited articles, one related to data envelopment analysis (DEA) and other that presents a wide variation of methods for benchmarking. There are important critiques towards the past research on benchmarking of best practices, yet, new DEA approaches seem to address most of the criticized issues.

Practical implications
From time to time, a review of the literature of a certain field of knowledge is useful for other researchers to identify and organize its main theories, new trends, most relevant works, influent authors and important publications.

Originality/value
This paper delivers two important values: the updated discussion on benchmarking of best practices, clearing both DEA and non-DEA studies; and the methods adopted to systematically review the literature, which is original on this field of research.
The effect of trust with suppliers on hospital supply chain performance
Authors: Abdallah Ayman Bahjat , Abdullah Mais Issam , Mahmoud Saleh Firas Izzat 
Published: March 2017

PurposeThe purpose of this paper is to investigate the effect of trust with suppliers on hospital-supplier integration (SI) and hospital supply chain (SC) performance. Additionally, the mediating effect of SI on trust-hospital SC performance is investigated.Design/methodology/approachA research model and hypotheses were developed based on literature review. The study is based on survey data collected from 152 respondents in 55 private hospitals in Jordan. The proposed hypotheses were tested using structural equation modeling.FindingsThe paper finds that trust with suppliers has a positive impact on hospital-SI and hospital SC performance. Hospital-SI partially mediates the relationship between trust and hospital SC performance.Practical implicationsThe findings of this research provide useful insights into the role of trust in boosting SC performance in the healthcare sector. High levels of SI not only improve hospital SC performance but also enhance the transformation of trust benefits into SC performance.Originality/valueThis research is one of the limited studies that investigated the effect of trust on hospital-SI and hospital SC performance in the healthcare sector. To the best of the authors’ knowledge, this is the first study to investigate those relationships in the Middle East in general and in Jordan in particular.
The emergence and evolution of benchmarking: a management fashion perspective
Authors: Madsen Dag Øivind , Slåtten Kåre , Johanson Daniel 
Published: March 2017

PurposeThe purpose of this paper is to make a contribution to the benchmarking literature by examining the historical emergence and evolution of benchmarking using the management fashion perspective as a theoretical lens.Design/methodology/approachThe research approach followed in this paper can be characterized as explorative and theoretical. Insights from different data sources have been combined to provide a rich description of the emergence and evolution of benchmarking.FindingsThis analysis casts new light on several aspects of benchmarking’s emergence and evolution pattern. The characteristics of the benchmarking idea give it potential as a fashionable management tool. The widespread popularity and longevity of benchmarking can to a large extent be explained by the efforts of various actors to turn benchmarking into an institution.Research limitations/implicationsThe paper is explorative and is limited by a reliance on secondary sources.Originality/valueAlthough some researchers have noted that benchmarking could be viewed as a management fashion, management fashion theory has, only to a very limited extent, been used as a theoretical lens in the context of benchmarking. This research paper demonstrates that management fashion theory can provide valuable insights for research on benchmarking.
Healthcare waste disposal strategy selection using grey-AHP approach
Authors: Thakur Vikas , Ramesh A. 
Published: March 2017

Purpose
Nowadays healthcare waste (HCW) has become a big challenge for the hospital management, especially, in developing countries like India. Like other developing countries, Indian healthcare waste disposal (HCWD) industry is also fragmented, as some hospitals are having their own in-house waste treatment facilities and others are outsourcing the process to government authorized Common Biomedical Waste Treatment Facilities. Literature also lacks the quantitative studies in selecting the HCWD strategy and, hence, the purpose of this paper is to identify and prioritize the factors, which affect the selection of HCWD strategy and to propose the model to select the HCWD strategy and to apply the proposed model to select the HCWD strategy in Uttarakhand, Northern State of India.

Design/methodology/approach
Grey theory-based analytic hierarchy process approach has been applied to evaluate the HCWD options and select the appropriate strategy for the healthcare facilities (HCFs).

Findings
From the literature, six criteria have been used to evaluate the HCWD strategies: “access to expertise,” “overdependence,” “transportation & risk associated,” “Government rules,” “environmental factors,” and “economic factors.” “Outsourcing” strategic option (0.61) got the higher desirability index than “in-house treatment” (0.39).

Research limitations/implications
The proposed methodology can be used by the researchers and experts in the field to evaluate the strategic options and select the appropriate strategy.

Practical implications
The HCFs and other generators of HCW can select the alternative, whether they should treat the infectious waste in-house or they should go for outsourcing.

Originality/value
In the field of HCW management, this is the first study of its kind, which helps to evaluate and select the HCWD strategies. The proposed model has been applied in Uttarakhand, Northern State of India to make the comparison between “in-house” and “outsource” strategies.
Foliated networks to analyze moral responsibility: a conceptual model
Authors: Eriksson David , Hilletofth Per 
Published: March 2017

PurposeThis study aims to explore how the flow of moral responsibility in supply chains can be understood through an analysis of material, monetary and information flows.Design/methodology/approachSocial responsibility, foliated networks and morality are used to present a conceptual framework that suggests responsibility links in supply chains.FindingsBy understanding the flows of material, money and information, it is possible to see how different types (liable and political) of responsibility can be identified. Conventional supply chain flows are thus connected with moral responsibility.Research limitations/implicationsResponsibility issues in supply chain management need to include supply chain links created by monetary and information flows, as well as material flows.Practical implicationsSupply chain actors need to consider responsibility across their entire supply chain, which includes material, monetary and information flows.Originality/valueFoliated transportation networks, moral disengagement and different types of responsibility are combined in a novel way to facilitate a better understanding of responsibility in supply chains.
“We have always been responsible”
Authors: Brunninge Olof , Fridriksson Helgi-Valur 
Published: March 2017

PurposeThe aim of this study is to discuss what implications referencing to the past can have on how firms manage their supply chains and communicate about them, drawing on the social memory literature.Design/methodology/approachIn a conceptual manner, the authors connect the field of responsible supply chain management to the growing literature on corporate heritage and social memory in organizations.FindingsThe authors develop seven propositions related to the communication of the past and its connection to responsible supply chain management.Research limitations/implicationsA social memory perspective can inform supply chain management research, by helping to better understand how and with what consequences the past can be used in communication about supply chains. This paper is conceptual in nature and empirical investigations would be needed to support and/or modify the literature-based findings.Practical implicationsManagers should be aware that both opportunities and risks are associated with communicating the past in connection to responsible supply chain management. Deployed in the right way, such communication can be valuable both in marketing and in internal management processes.Originality/valueThis article introduces the social memory perspective to the supply chain management field and shows what implications it can have for research on responsibility in supply chains.
Professionalism as social responsibility in procurement and administration
Authors: Steinfeld Joshua , McCue Clifford , Prier Eric 
Published: March 2017

Purpose
The purpose of this empirical study is to identify the job tasks where decisions regarding social responsibility are likely to occur and assess the potential connections between social responsibility and professionalism.

Design/methodology/approach
A job study conducted by the Universal Public Procurement Certification Council (UPPCC) of 2,593 practitioners is used for data collection. Factor analysis is applied to a set of 75 procurement job tasks to determine the relationship between practitioners’ performance and management of job tasks and social responsibility variables.

Findings
The results suggest that there are specific job tasks performed and managed in both public and private sector procurement that share a unique relationship with social responsibility variables.

Research limitations/implications
The manuscript advances the research on professionalism in procurement and administration through empirically testing job tasks performed and managed by practitioners and identifying relationships between job tasks according to a professional orientation toward social responsibility.

Practical implications
The study shows that specific job tasks are performed and managed in procurement and administration with a social responsibility consideration.

Social implications
The technical nature of job tasks found to be related to social responsibility suggests a paradoxical view of the politics-administration dichotomy, and the notion that neutral tasks of both the public and private sectors are not void of a social function.

Originality/value
One attribute of professionalism in the literature, social responsibility, is operationalized through actual performance and management of job tasks by practitioners.
Towards socially sustainable supply chains – themes and challenges
Authors: Abbasi Maisam 
Published: March 2017

Purpose
The purpose of this article is to explore and classify the pattern of themes and challenges in developing socially sustainable supply chains.

Design/methodology/approach
The methodology is based on a systematic review of the peer-reviewed literature to explore what major themes and challenges have been discussed and the significant gaps where opportunities for further research can be found.

Findings
In total, four categories of themes were identified, namely, human-centric, focal organization-centric, supply chain-centric and governance-centric. Challenges were classified into seven categories, namely, inadequate and asymmetric knowledge, difficulties of operationalization, shifting the values, subjectivity in evaluation, governance complexity, difficulties of small- and medium-sized enterprises and sustainability fade.

Research limitations/implications
The focus of the article is on the social pillar of sustainable development in the context of supply chains. A more holistic systematic investigation of synergy of all the three pillars/bottom lines of sustainable development (economic, environmental and social) can be an opportunity for further research.

Practical implications
Taking a more holistic view of the pattern of currently discussed themes and challenges may be beneficial in increasing the absorptive capacity of industrial and business practitioners, by accumulating and assimilating external knowledge, when they design and operationalize innovative strategies in developing sustainable supply chains.

Originality/value
This article may increase awareness about the social responsibilities of supply chains actors and stakeholders in different scales. It may also guide managers, decision makers and practitioners to better understand the difficulties, obstacles or dilemmas that can hinder the sustainable development of supply chains. The results section presents a framework driven from the emerged themes, and the discussion section provides propositions for tackling the challenges and opportunities for further research.