bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rapid Method for the Quantitative Determination of 34 Pesticides in Nonalcoholic Carbonated Beverages Using Liquid–Liquid Extraction Coupled to Dispersive Solid-Phase Cleanup Followed by Gas Chromatography with Tandem Mass Spectrometry
Authors: Rai Satyajeet , Gullapalli Madhuri Devi , Srivastava Anshuman , Shaik Hussain , Siddiqui Mohammed Haris , Mudiam Mohana Krishna Reddy 
Published: March 2017

Abstract
An economical, rapid, and sensitive multiresidue method using liquid–liquid extraction (LLE) coupledwith dispersive SPE (dSPE) cleanup was developed for the quantitative determination of 34 multiclass multiresidue (MCMR) pesticides (14 organochlorines, eight organophosphates, 10 synthetic pyrethroids, and two herbicides) in nonalcoholic carbonated beverages (cola, orange, lemon-lime, and citra) using GC with tandem MS. The procedure mainly involved LLE by dichloromethane and dSPE cleanup in the presence of magnesium sulfate, primary secondary amine, and C18. The RSD of the developed method was found to be less than 14%. The LOD andLOQ values for all the analyzed pesticides were found in the ranges of 0.001–0.027 μg/L and 0.004–0.088 μg/L, respectively. The LOQ levels of the pesticides analyzed were found to be well below the recommended limit by the European Union (0.1 μg/L in water). The mean recoveries of pesticides indifferent nonalcoholic carbonated beverages (cola, orange, lemon-lime, and citra) were found to be in the range of 79–111%, with RSDs less than 11%. The validation data prove that the method can be acceptable to regulatory agencies for the routine analysis of MCMR pesticides in nonalcoholic carbonated beverages.
Estereotipos corporales en las imágenes de los libros de texto de inglés
Authors: Menescardi Royela Cristina , Estevan Torres Isaac , Ros Ros Concepción , Moya-Mata Irene 
Published: March 2017

<p>La educación es el camino para generar conductas positivas en niños y niñas y promover un ambiente social y armónico, así como respeto de los valores éticos y democráticos de nuestra sociedad. Un valor ético y democrático importante es la actitud no discriminante, sin prejuicios y no estereotipada. El objetivo del presente estudio fue analizar la frecuencia de ocurrencia de posibles estereotipos ligados a género, edad, raza y somatotipo presentes en las imágenes de los libros de texto de inglés del tercer ciclo de Primaria del sistema educativo español. Para ello, se ha realizado un estudio descriptivo con 1774 imágenes de 5 colecciones de libros de texto de la asignatura de inglés (Bugs World, Incredible Kit, Footstep, Beep and Find Out) de quinto y sexto curso. Los resultados obtenidos mostraron una continuación de los estereotipos de cuerpos jóvenes, de raza blanca y delgados en las imágenes de los libros de texto de inglés, mientras que los estereotipos de género parecen ser eliminados de dichos libros a favor de una mayor diversidad en sus imágenes. Se debería tomar en consideración la necesidad de que el profesorado revise el material didáctico y en el caso de que no se asegure el valor de la diversidad cultural e igualdad de oportunidades de cada estudiante, se elabore su propio material curricular complementario. En este sentido, se aporta una propuesta didáctica y recomendaciones aplicadas para establecer las bases del trabajo con imágenes en la etapa de Primaria para identificar, reconocer y eliminar los estereotipos.</p>
Investigación Basada en las Artes como Investigación Educativa: análisis de una experiencia en el Colegio San Isidro en Guadalén
Authors: Moreno-Montoro María-Isabel , Tirado-de-la-Chica Ana , López-Peláez-Casellas María-Paz , Martínez-Morales María 
Published: March 2017

<p>Este artículo da cuenta de una Investigación Basada en las Artes (IBA) llevada a cabo en un centro de Educación Infantil y Primaria. Un proyecto de creación con artistas sirve de acontecimiento para poner en marcha un trabajo de campo desde la IBA. El centro es el Colegio San Isidro de Guadalén en la provincia de Jaén, España, y el proyecto en el que se implementa la investigación es un Erasmus +, coordinado desde la Universidad de Oporto y en<br />el que participamos como socios desde la Universidad de Jaén. En el artículo comenzamos justificando el interés que nos mueve a considerar necesaria la investigación sobre resultados que aporta la práctica artística contemporánea en la educación, para seguir por una contextualización teórica donde explicaremos como afrontamos la Investigación Basada en las Artes y las prácticas artísticas contemporáneas en la escuela. Seguidamente describiremos el proceso de investigación y el trabajo de campo desarrollados, en el que daremos cuenta de cómo los artistas Lucía Loren y Juanma Valentín llevan a cabo un proyecto en colaboración con toda la comunidad educativa del colegio. Acabaremos el artículo con una redacción de pensamientos finales a modo de discusión, elaborados a partir de los resultados tras la experiencia artística llevada en el centro en los que se pone de manifiesto fundamentalmente como la investigación educativa es favorecida por la acción artística en la escuela cuando se trata desde los procesos contemporáneos.</p>
Educación, psicología y coaching: un entramado positivo
Authors: Sandoval Lentisco Cristina , López Martínez Olivia 
Published: March 2017

<p>La psicología positiva está alcanzando un progresivo auge, no solo en el ámbito clínico, sino también en el ámbito educativo. De hecho, podemos encontrar experiencias pioneras en este ámbito donde se afirman las ventajas de una educación basada en la pedagogía de la felicidad (Carpena, 2014). Educar bajo los pilares de la psicología positiva (emociones, fortalezas- virtudes e institución positiva), junto a los elementos que constituyen la<br />teoría del bienestar es esencial para potenciar la resiliencia y minimizar el dolor emocional que se genere a lo largo de la vida. Sin embargo, ¿todas las prácticas que promueven lo positivo es psicología positiva? En la actualidad existen algunas creencias erróneas sobre su concepto y<br />campo de intervención. En algunos casos, se ha simplificado su teoría asemejándola al pensamiento positivo que abogan algunos manuales de autoayuda. En otras ocasiones, se ha confundido con sesiones de coaching emocional a través de los planes de entrenamiento personal-emocional que ésta promueve. Si nos trasladamos al ámbito educativo, podemos observar cómo ésta se ha entremezclado con las teorías y prácticas de la educación emocional y la inteligencia emocional. Ante la diversidad de opiniones y creencias erróneas sobre esta rama de la psicología, se propone desmitificar tales mitos, esclarecer dichos conceptos para así abrir paso a la praxis de la verdadera psicología positiva en los centros educativos. Se concluye estableciendo las similitudes y diferencias de estas áreas de trabajo frente la psicología positiva a fin de esclarecer el entramado positivo que nos invade en la actualidad.</p>
Postnatal HIV transmission in breastfed infants of HIV‐infected women on ART: a systematic review and meta‐analysis
Authors: Bispo Stephanie , Chikhungu Lana , Rollins Nigel , Siegfried Nandi , Newell Marie‐Louise 
Published: March 2017

AbstractIntroduction: To systematically review the literature on mother‐to‐child transmission in breastfed infants whose mothers received antiretroviral therapy and support the process of updating the World Health Organization infant feeding guidelines in the context of HIV and ART.Methods: We reviewed experimental and observational studies; exposure was maternal HIV antiretroviral therapy (and duration) and infant feeding modality; outcomes were overall and postnatal HIV transmission rates in the infant at 6, 9, 12 and 18 months. English literature from 2005 to 2015 was systematically searched in multiple electronic databases. Papers were analysed by narrative synthesis; data were pooled in random effects meta‐analyses. Postnatal transmission was assessed from four to six weeks of life. Study quality was assessed using a modified Newcastle‐Ottawa Scale (NOS) and GRADE.Results and discussion: Eleven studies were identified, from 1439 citations and review of 72 abstracts. Heterogeneity in study methodology and pooled estimates was considerable. Overall pooled transmission rates at 6 months for breastfed infants with mothers on antiretroviral treatment (ART) was 3.54% (95% CI: 1.15–5.93%) and at 12 months 4.23% (95% CI: 2.97–5.49%). Postnatal transmission rates were 1.08 (95% CI: 0.32–1.85) at six and 2.93 (95% CI: 0.68–5.18) at 12 months. ART was mostly provided for PMTCT only and did not continue beyond six months postpartum. No study provided data on mixed feeding and transmission risk.Conclusions: There is evidence of substantially reduced postnatal HIV transmission risk under the cover of maternal ART. However, transmission risk increased once PMTCT ART stopped at six months, which supports the current World Health Organization recommendations of life‐long ART for all.
Endovascular Ablation of the Greater Splanchnic Nerve in Heart Failure With Preserved Ejection Fraction
Authors: Fudim Marat , Borlaug Barry A. , Mohan Rajeev C. , Price Matthew J. , Fail Peter , Goyal Parag , Hummel Scott L. , Zirakashvili Teona , Shaburishvili Tamaz , Patel Ravi B. , Reddy Vivek Y. , Nielsen Christopher D. , Chetcuti Stanley J. , Sukul Devraj , Gulati Rajiv , Kim Luke , Benzuly Keith , Mitter Sumeet S. , Klein Liviu , Uriel Nir , Augostini Ralph S. , Blair John E. , Rocha-Singh Krishna , Burkhoff Daniel , Patel Manesh R. , Somo Sami I. , Litwin Sheldon E. , Shah Sanjiv J. 
Published: October 2024

ImportanceGreater splanchnic nerve ablation may improve hemodynamics in patients with heart failure and preserved ejection fraction (HFpEF).ObjectiveTo explore the feasibility and safety of endovascular right-sided splanchnic nerve ablation for volume management (SAVM).Design, Setting, and ParticipantsThis was a phase 2, double-blind, 1:1, sham-controlled, multicenter, randomized clinical trial conducted at 14 centers in the US and 1 center in the Republic of Georgia. Patients with HFpEF, left ventricular ejection fraction of 40% or greater, and invasively measured peak exercise pulmonary capillary wedge pressure (PCWP) of 25 mm Hg or greater were included. Study data were analyzed from May 2023 to June 2024.InterventionSAVM vs sham control procedure.Main Outcomes and MeasuresThe primary efficacy end point was a reduction in legs-up and exercise PCWP at 1 month. The primary safety end point was serious device- or procedure-related adverse events at 1 month. Secondary efficacy end points included HF hospitalizations, changes in exercise function and health status through 12 months, and baseline to 1-month change in resting, legs-up, and 20-W exercise PCWP.ResultsA total of 90 patients (median [range] age, 71 [47-90] years; 58 female [64.4%]) were randomized at 15 centers (44 SAVM vs 46 sham). There were no differences in adverse events between groups. The primary efficacy end point did not differ between SAVM or sham (mean between-group difference in PCWP, −0.03 mm Hg; 95% CI, −2.5 to 2.5 mm Hg; P = .95). There were also no differences in the secondary efficacy end points. There was no difference in the primary safety end point between the treatment (6.8% [3 of 44]) and sham (2.2% [1 of 46]) groups (difference, 4.6%; 95% CI, −6.1% to 15.4%; P = .36). There was no difference in the incidence of orthostatic hypotension between the treatment (11.4% [5 of 44]) and sham (6.5% [3 of 46]) groups (difference, 4.9%; 95% CI, −9.2% to 18.8%; P = .48).Conclusions and RelevanceResults show that SAVM was safe and technically feasible, but it did not reduce exercise PCWP at 1 month or improve clinical outcomes at 12 months in a broad population of patients with HFpEF.Trial RegistrationClinicalTrials.gov Identifier: NCT04592445
Lithium Aspartate for Long COVID Fatigue and Cognitive Dysfunction
Authors: Guttuso Thomas , Zhu Jingtao , Wilding Gregory E. 
Published: October 2024

ImportanceNeurologic post–COVID-19 condition (PCC), or long COVID, symptoms of fatigue and cognitive dysfunction continue to affect millions of people who have been infected with SARS-CoV-2. There currently are no effective evidence-based therapies available for treating neurologic PCC.ObjectiveTo assess the effects of lithium aspartate therapy on PCC fatigue and cognitive dysfunction.Design, Setting, and ParticipantsA randomized, double-blind, placebo-controlled trial (RCT) enrolling participants in a neurology clinic from November 28, 2022, to June 29, 2023, with 3 weeks of follow-up, was conducted. Subsequently, an open-label lithium dose-finding study with 6 weeks of follow-up was performed among the same participants enrolled in the RCT. Eligible individuals needed to report new, bothersome fatigue or cognitive dysfunction persisting for more than 4 weeks after a self-reported positive test for COVID-19, Fatigue Severity Scale-7 (FSS-7) or Brain Fog Severity Scale (BFSS) score of 28 or greater, Beck Depression Inventory-II score less than 29, and no history of a condition known to cause fatigue or cognitive dysfunction. All participants in the RCT were eligible for the dose-finding study, except for those who responded to the placebo. Intention-to-treat analysis was used.InterventionLithium aspartate, 10 to 15 mg/d, or identically appearing placebo for 3 weeks followed by open-label lithium aspartate, 10 to 15 mg/d, for 2 weeks. In the subsequent dose-finding study, open-label lithium aspartate dosages up to 45 mg/d for 6 weeks were given.Main Outcomes and MeasuresChange in sum of FSS-7 and BFSS scores. The scores for each measure range from 7 to 49, with higher scores indicating more severe symptoms. Secondary outcomes included changes from baseline in the scores of additional questionnaires.ResultsFifty-two participants were enrolled (30 [58%] males; mean [SD] age, 58.54 [14.34] years) and 26 were randomized to treatment with lithium aspartate (10 females) and 26 to placebo (12 female). Two participants assigned to lithium aspartate were lost to follow-up and none withdrew. No adverse events were attributable to lithium therapy. There were no significant intergroup differences for the primary outcome (−3.6; 95% CI, −16.6 to 9.5; P = .59) or any secondary outcomes. Among 3 patients completing a subsequent dose-finding study, open-label lithium aspartate, 40 to 45 mg/d, was associated with numerically greater reductions in fatigue and cognitive dysfunction scores than 15 mg/d, particularly in 2 patients with serum lithium levels of 0.18 and 0.49 mEq/L compared with 1 patient with a level of 0.10 mEq/L.Conclusions and RelevanceIn this RCT, therapy with lithium aspartate, 10 to 15 mg/d, was ineffective for neurologic PCC fatigue and cognitive dysfunction. Another RCT is required to assess the potential benefits of higher lithium dosages for treating neurologic PCC.Trial RegistrationClinicalTrials.gov Identifier: NCT05618587 and NCT06108297
Flexible, Conductive, and Transparent Polytetrafluoroethylene/AgPdCu/InZnO Hybrid Electrodes for Transparent and Flexible Thin‐Film Heaters
Authors: Cheon Hosung , Park So Mang , Lee Ye Seo , Kim Han‐Ki 
Published: October 2024

AbstractHighly flexible, transparent, and conductive polytetrafluoroethylene (PTFE)/AgPdCu (APC)/indium zinc oxide (IZO) hybrid films are developed for use in transparent and flexible thin‐film heaters (FTFHs) integrated into electric vehicles. By controlling the thickness of each layer, PTFE/APC/IZO multilayer electrodes with a low sheet resistance of 9.73 Ω/sq and high transmittance of 80.08% at a wavelength of 550 nm and outstanding mechanical flexibility even though it is prepared at room temperature is obtained. In multilayer electrodes, each component plays a specific role, this is, IZO acts as a transparent barrier, APC is the main conduction path, and PTFE is the adhesive layer. Notably, the optimal PTFE/APC/IZO multilayer exhibited superior mechanical flexibility compared with that of the IZO/Ag/IZO multilayer owing to the soft PTFE adhesive layer. The FTFH with the optimized PTFE/APC/IZO multilayer demonstrated a high saturation temperature of 101.9 °C at a low input voltage of 12 V owing to the low sheet resistance and stability provided by the IZO barrier layer. Therefore, the PTFE/APC/IZO hybrid electrodes prepared via a continuous sputtering process provide an effective strategy for achieving high‐performance transparent FTFHs for next‐generation electric vehicles.
Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis
Authors: Kang Ju‐Eun , Kim Hansol , Lee Young‐Hoon , Lee Ha‐Yeong , Park Yeonkyung , Jang Hyowon , Kim Jae‐Rin , Lee Min‐Young , Jeong Byeong‐Ho , Byun Ju‐Young , Kim Seung Jun , Lim Eun‐Kyung , Jung Juyeon , Woo Eui‐Jeon , Kang Taejoon , Park Kwang‐Hyun 
Published: October 2024

AbstractCas12j, a hypercompact and efficient Cas protein, has potential for use in CRISPR diagnostics, but has not yet been used because the trans‐cleavage activity of Cas12j is veiled. Here, the trans‐cleavage behavior of Cas12j1, 2, and 3 variants and evaluate their suitability for nucleic acid detection is unveiled. The target preferences and mismatch specificities of the Cas12j variants are precisely investigated and the optimal Cas12j reaction conditions are determined. As a result, the EXP‐J assay for miRNA detection by harnessing the robust trans‐cleavage activity of Cas12j on short ssDNA is developed. The EXP‐J method demonstrates exceptional detection capabilities for miRNAs, proving that Cas12j can be a pivotal component in molecular diagnostics. Furthermore, the translational potential of the EXP‐J assay is validated by detecting oncogenic miRNAs in plasma samples from lung cancer patients. This investigation not only elucidates the trans‐cleavage characteristics of Cas12j variants, but also advances the Cas12j‐based diagnostic toolkit.
Targeting Immunoproteasome in Polarized Macrophages Ameliorates Experimental Emphysema Via Activating NRF1/2‐P62 Axis and Suppressing IRF4 Transcription
Authors: Guo Bingxin , Shi Xing , Jiang Qiong , Pan Yuanwei , Yang Yuqiong , Liu Yuanyuan , Chen Shuyu , Zhu Wenjiao , Ren Laibin , Liang Ruifang , Chen Xue , Xu Haizhao , Wei Laiyou , Lin Yongjian , Wang Jinyong , Qiu Chen , Zhou Haibo , Rao Lang , Wang Lingwei , Chen Rongchang , Chen Shanze 
Published: October 2024

AbstractChronic obstructive pulmonary disease (COPD) stands as the prevailing chronic airway ailment, characterized by chronic bronchitis and emphysema. Current medications fall short in treatment of these diseases, underscoring the urgent need for effective therapy. Prior research indicated immunoproteasome inhibition alleviated various inflammatory diseases by modulating immune cell functions. However, its therapeutic potential in COPD remains largely unexplored. Here, an elevated expression of immunoproteasome subunits LMP2 and LMP7 in the macrophages isolated from mouse with LPS/Elastase‐induced emphysema and polarized macrophages in vitro is observed. Subsequently, intranasal administration of the immunoproteasome‐specific inhibitor ONX‐0914 significantly mitigated COPD‐associated airway inflammation and improved lung function in mice by suppressing macrophage polarization. Additionally, ONX‐0914 capsulated in PLGA nanoparticles exhibited more pronounced therapeutic effect on COPD than naked ONX‐0914 by targeting immunoproteasome in polarized macrophages. Mechanistically, ONX‐0914 activated autophagy and endoplasmic reticulum (ER) stress are not attribute to the ONX‐0914 mediated suppression of macrophage polarization. Intriguingly, ONX‐0914 inhibited M1 polarization through the nuclear factor erythroid 2‐related factor‐1 (NRF1) and NRF2‐P62 axis, while the suppression of M2 polarization is regulated by inhibiting the transcription of interferon regulatory factor 4 (IRF4). In summary, the findings suggest that targeting immunoproteasome in macrophages holds promise as a therapeutic strategy for COPD.
Extreme Electron‐Photon Interaction in Disordered Perovskites
Authors: Kharintsev Sergey S. , Battalova Elina I. , Matchenya Ivan A. , Nasibulin Albert G. , Marunchenko Alexander A. , Pushkarev Anatoly P. 
Published: October 2024

AbstractThe interaction of light with solids can be dramatically enhanced owing to electron‐photon momentum matching. This mechanism manifests when light scattering from nanometer‐sized clusters including a specific case of self‐assembled nanostructures that form a long‐range translational order but local disorder (crystal‐liquid duality). In this paper, a new strategy based on both cases for the light‐matter‐interaction enhancement in a direct bandgap semiconductor – lead halide perovskite CsPbBr3 – by using electric pulse‐driven structural disorder, is addressed. The disordered state allows the generation of confined photons, and the formation of an electronic continuum of static/dynamic defect states across the forbidden gap (Urbach bridge). Both mechanisms underlie photon‐momentum‐enabled electronic Raman scattering (ERS) and single‐photon anti‐Stokes photoluminescence (PL) under sub‐band pump. PL/ERS blinking is discussed to be associated with thermal fluctuations of cross‐linked [PbBr6]4‐ octahedra. Time‐delayed synchronization of PL/ERS blinking causes enhanced spontaneous emission at room temperature. These findings indicate the role of photon momentum in enhanced light‐matter interactions in disordered and nanostructured solids.
Widespread transposable element dysregulation in human aging brains with Alzheimer's disease
Authors: Feng Yayan , Yang Xiaoyu , Hou Yuan , Zhou Yadi , Leverenz James B. , Eng Charis , Pieper Andrew A. , Goate Alison , Shen Yin , Cheng Feixiong 
Published: October 2024

AbstractINTRODUCTIONTransposable element (TE) dysregulation is associated with neuroinflammation in Alzheimer's disease (AD) brains. Yet, TE quantitative trait loci (teQTL) have not been well characterized in human aged brains with AD.METHODSWe leveraged large‐scale bulk and single‐cell RNA sequencing, whole‐genome sequencing (WGS), and xQTL from three human AD brain biobanks to characterize TE expression dysregulation and experimentally validate AD‐associated TEs using CRISPR interference (CRISPRi) assays in human induced pluripotent stem cell (iPSC)–derived neurons.RESULTSWe identified 26,188 genome‐wide significant TE expression QTLs (teQTLs) in human aged brains. Subsequent colocalization analysis of teQTLs with AD genetic loci identified AD‐associated teQTLs and linked locus TEs. Using CRISPRi assays, we pinpointed a neuron‐specific suppressive role of the activated short interspersed nuclear element (SINE; chr11:47608036–47608220) on expression of C1QTNF4 via reducing neuroinflammation in human iPSC‐derived neurons.DISCUSSIONWe identified widespread TE dysregulation in human AD brains and teQTLs offer a complementary analytic approach to identify likely AD risk genes.Highlights
Widespread transposable element (TE) dysregulations are observed in human aging brains with degrees of neuropathology, apolipoprotein E (APOE) genotypes, and neuroinflammation in Alzheimer's disease (AD).
A catalog of TE quantitative trait loci (teQTLs) in human aging brains was created using matched RNA sequencing and whole‐genome sequencing data.
CRISPR interference assays reveal that an upregulated intergenic TE from the MIR family (chr11: 47608036–47608220) suppresses expression of its nearest anti‐inflammatory gene C1QTNF4 in human induced pluripotent stem cell–derived neurons.