bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional characterization of chalcone reductase gene CHR3 through RNAi
Authors: Wu Nan , Jiang Long , Yu Kexin , Ning Xilin , Liu Ming , Yuan Minghao 
Published: October 2024

In this experiment, the soybean genome "Jilin 30" was used as a template to clone the target gene CHR3 using specific primers. The sense fragment, antisense fragment, and intron of the target gene were ligated into pCAMBIA3300 through double enzyme digestion to form a stem loop structure. We then transferred the constructed RNAi vector pCAMBIA3301-CHR3 RNAi into the recipient soybean genome via Agrobacterium mediated method, specifically reducing the expression of the target gene CHR3. By genetic transformation, positive plants were obtained, and molecular biology methods such as Southern blotting, real-time quantitative PCR, isoliquiritigenin analysis, and identification of resistance to Phytophthora root rot were used to detect the expression level of the CHR3 gene in the positive plants and study its function.The RNAi expression vector pCAMBIA3300-CHR3-RNAi was successfully constructed using the sense and antisense fragments of 428 bp CHR3 gene and an intron of 110 bp was inserted to form the stem-loop structure of the RNAi vector. PCR was used to detect 6 lines of the T1 generation and 15 lines of the T2 generation. The results of Southern analysis confirmed the integration of CHR3 and marker genes into the soybean genome. The expression CHR3 gene in the T1 genotypes decreased by 13.1 to 58.3%; the expression in the root decreased by 0.9 to 47.4%; the expression in the leaf decreased by 20 to 44.2%; and the expression in the stem decreased by 7.6 to 23%. The content of isoliquiritigenin decreased by 26.7% as transgenic shoots had a lower content of isoliquiritigenin and reduced resistance to Phytophthora sojae.
Bangladesh J. Bot. 53(3): 627-633, 2024 (September) Special
Determinants of Caesarean Deliveries in Bangladesh: A Multilevel Analysis of a Nationwide Population-Based Survey
Authors: Ireen Shahjadi , Hasan Md Zahid , Akter Salma , Tareq Muhammad , Begum Mansura , Sultana Rebeka , Parvin Shahanaj , Soni Most Sifat Muntaha 
Published: October 2024

Caesarean section, a frequently conducted surgical procedure in obstetrics, is instrumental in safeguarding the well-being of both mothers and infants by addressing complications related to pregnancy and childbirth. While it significantly contributes to reducing maternal and infant mortality rates, its overuse can pose serious health risks. The prevalence of caesarean deliveries has surged globally, including in Bangladesh. This rise in caesarean deliveries has prompted this research to investigate the prevalence and various associated factors in the country. To achieve the study objectives, data were extracted from the Bangladesh Demographic and Health Survey (BDHS) conducted from 2017 to 2018. After preprocessing the data, a total of 4,884 women were included in the study. Frequency analysis was conducted to illustrate the distribution of sociodemographic characteristics among Bangladeshi women. Chi-square tests were performed to assess their association with caesarean delivery. Furthermore, multi-level logistic regression analysis was employed to identify the variables that have a significant impact on the likelihood of caesarean delivery. The study revealed that in Bangladesh, the prevalence of caesarean section deliveries was approximately 33.1%, with the majority of these occurring in the Dhaka division, accounting for approximately 25.3%. Women aged 20 or older were 1.62 times more likely to opt for a C-section during childbirth (OR 1.62, [1.38-1.89]) compared to those under 20. Overweight or obese women had twice the likelihood of delivering via C-section. Higher socioeconomic status also increased the probability of choosing a caesarean delivery. Women who had exposure to any form of media were 57% more likely to opt for a caesarean delivery (OR 1.57 [1.31-1.87]), while not working women had a 48% higher likelihood of having a C-section. Additionally, the study found that the use of caesarean delivery was positively associated with the husband's education level, the number of children previously born, and the number of antenatal care visits. The study revealed that the rate of C-sections in Bangladesh surpasses WHO recommendations significantly. It also identified various factors driving this trend, including women's education levels, socioeconomic status, media exposure, female obesity, age at first childbirth, and husband’s education. These insights may serve as a foundation for developing strategies to regulate the caesarean delivery rate. Urgent policy directives are essential within Bangladesh's healthcare system, coupled with vigilant monitoring of clinical indications for C-sections, to mitigate the adverse effects of unnecessary procedures.
Jagannath University Journal of Science, Volume 11, Number 1, June 2024, pp. 31−44
PENDAMPINGAN IBU HAMIL ANEMIA DAN KEKURANGAN ENERGI KRONIS (KEK) DI DESA JARAK KECAMATAN PLOSOKLATEN KABUPATEN KEDIRI
Authors: Aisyah Siti 
Published: October 2024

Berdasarkan data dari Pemantauan wilayah Setempat Kesehatan Ibu dan Anak (PWS-KIA) Desa Jarak tahun 2017 didapatkan 24 ibu hamil risiko tinggi, 8 diantaranya dengan anemia dan 2 orang KEK. KEK dan anemia merupakan faktor risiko perdarahan post partum dan Berat Badan Lahir Rendah(BBLR).Tujuan dari kegiatan ini adalah melakukan pendampingan ibu hamil yang anemia dan KEK dari hamil sampai nifas dengan melibatkan peran serta masyarakat dan keluarga. Metode pendekatan yang digunakan adalah kerjasama lintas program dengan puskesmas, kerjasama lintas sektor dengan aparat desa, pemberdayaan kader dan keluarga ibu hamil. Kegiatan yang dilakukan adalah melakukan pemerikasaan kehamilan, memberikan suplemen tablet Fe, melakukan penyuluhan tentang gizi pada masa kehamilan, mengadakan kelas ibu hamil, melakukan kunjungan rumah untuk memantau kondisi ibu hamil serta melatih keluarga sebagai pendamping minum Fe dan pemenuhan gizi ibu hamil. Hasil kegiatan semua ibu hamil yang anemia dapat tertangani, proses persalinan 6 ibu hamil melahirkan secara normal di bidan desa dan 2 ibu hamil dirujuk ke RSUD Pare tepat waktu, ibu dan bayi dalam kondisi sehat. Masa nifas 8 ibu hamil berjalan normal. Mengatasi masalah kehamilan dengan kasus anemia dan KEK tidak bisa hanya upaya preventif dari petugas kesehatan saja, peran masyarakat utamanya kader kesehatan dan pemberdayaan keluarga dalam penanganan menjadi kunci keberhasilan dalam pendampingan ibu hamil yang mengalami anemia dan KEK.
PENDIDIKAN KOMUNITAS TENTANG PEMAKAIAN BENGKUNG PADA IBU NIFAS DI DESA KELING KECAMATAN KEPUNG KABUPATEN KEDIRI
Authors: Rahayu Dewi Taurisiawati 
Published: October 2024

Perawatan pada masa nifas akan menentukan status kesehatan dan mempercepat proses pemulihan ibu setelah melahirkan. Budaya bengkung di Kabupaten Kediri masih kental sampai sat ini. Hampir semua ibu nifas di Desa Keling masih menggunakan bengkung atau stagen untuk dibebatkan di perutnya. Alasan yang disampaikan oleh ibu nifas tentang penggunaan bengkung itu adalah 10 orang ibu nifas ( 41, 67 %) mengatakan mereka memakai bengkung karena anjuran orang tua dan budaya dan 14 orang ibu nifas ( 58,335) mengatakan mereka memakai bengkung karena merasa nyaman. Tujuan dari kegiatan ini adalah untuk mengajarkan ibu nifas cara memakai bengkung yang aman agar tidak membahayakan proses pemulihan kesehatannya. Metode yang dipakai adalah survey untuk menentukan sasaran, pelatihan kader tentang cara pemakaian bengkung yang aman untuk ibu nifas. Kegiatan dilaksanakan tanggal 5-27 November 2017. Hasil dari kegiatan ini terdapat 24 ibu di Desa Keling yang menggunakan bengkung. Sebelum penyuluhan dilaksanakan ibu nifas menyampaikan beberapa keluhan karena pemakaian bengkung yang salah yaitu 9 ibu nifas mengatakan kakinya bengkak (37,5 %), 10 ibu nifas mengatakan mengalami gatal-gatal di kulit (41,67%), 3 orang ibu nifas merasakan sesak nafas (12,5%) dan 2 orang mengatakan tidak ada keluhan sama sekali. Dengan cara pemakaian yang salah pemakaian bengkung justru akan merugikan ibu nifas itu sendiri. Karena itulah dilaksanakan pendidikan komunitas tentang pemakaian bengkung yang benar kepada kader dan para ibu nifas di Desa Keling Kecamatan Kepung Kabupaten Kediri. Dengan pemakaian bengkung yang benar maka bisa membuat ibu nifas merasa nyaman tanpa mengganggu proses pemulihan kesehatannya.
PENDAMPINGAN UMKM DALAM PENGELOLAAN REMPAH HASIL PEKARANGAN PANGAN LESTARI (P2L) MENJADI PRODUK JAMU CELUP TRADISIONAL DI DESA SENGGURUH KABUPATEN MALANG
Authors: FAJAR RATNA FAJARWATI MEDITAMA 
Published: October 2024

Desa Sengguruh memiliki Pekarangan Pangan Lestari (P2L) yang di fokuskan di lingkungan RT 23 karena terdapat lahan pekarangan yang cukup luas. Beberapa tanaman yang terdapat di kawasan P2L ini sangat beragam, utamanya berisi beberapa tanaman obat keluarga (TOGA) salah satunya adalah rempah-rempah. Di masa pandemi covid 19 ini rempah-rempah menjadi alternatif jamu tradisional yang dapat meningkatkan sistem kekebalan tubuh dalam melawan virus yang masuk kedalam tubuh. Disamping itu sejak merebaknya covid-19 terjadilah penurunan omzet pelaku UMKM yang sangat signifikan. Oleh sebab itu rempah-rempah dari hasil Pekarangan Pangan Lestari (P2L) dapat dijadikan sebagai solusi untuk produk UMKM jamu celup tradisional dengan kemasan yang layak dijual dipasaran yang dapat menambah pemasukan rumah tangga bagi warga Desa Sengguruh dan dapat mandiri secara ekonomi di masa pandemi covid 19 ini. Tujuan dari pengabdian ini adalah meberikan pelatihan pembuatan jamu celup tradisional yang praktis dan bernilai tinggi kepada beberapa UMKM jamu hergal tradisional di Desa Sengguruh. Metode yang digunakan dalam pelatihan ini adalah memberikan edukasi mengenai cara membuat jamu celup tradisional dan variasi olahan jamu dengan rsa dan warna yang menarik. Hasil dari pelatihan ini adalah sebuah produk jamu celup dengan tiga varian rasa dan warna yaitu merah, kuning dan ungu.
KULIAH KERJA NYATA (KKN) TEMATIK MEMBUDAYAKAN HIDUP SEHAT BAGI ANAK-ANAK UNTUK MENCEGAH PENYEBARAN COVID-19 DI DESA NGLIMAN KECAMATAN SAWAHAN KABUPATEN NGANJUK
Authors: Oktaviola Rizka Dwi 
Published: October 2024

Kuliah Kerja Nyata (KKN) Kabupaten Nganjuk dengan judul KKN “Membudayakan Hidup Sehat Bagi Anak-anak Untuk Mencegah Penyebaran Covid-19 Di Desa Ngliman Tematik merupakan program yang diadakan setiap tahun di Universitas Islam Raden Rahmat Malang. Salah satu KKN Tematik Unira dilaksanakan di Desa Ngliman, Kecamatan Sawahan, Kecamatan Sawahan Kabupaten Nganjuk”. Desa Ngliman berada di Kecamatan Sawahan, Kabupaten Nganjuk yang berada di atas permukaan laut, dengan topografi dataran tinggi dan suhu udara rata-rata 17°celcius. Desa Ngliman memiliki luas sekitar 268 ha/m2 yang terletak di dataran tinggi yang berada pada ketinggian 1.300 meter. KKN Tematik Unira di Desa Ngliman berjumlah 1 orang mahasiswa Unira. Pada desa tersebut dilaksanakan program kerja yang telah dibuat oleh mahasiswa dengan persetujuan Dosen Pembimbing Lapangan (DPL) berdasarkan permasalaan dan potensi yang ada di desa tersebut. Program kerja keseluruhan pada KKN Tematik di Desa Ngliman berjumlah 4 program, dan program tersebut terlaksana semua
PELATIHAN PEMBUATAN VIDEO PEMBELAJARAN ONLINE (APLIKASI AZ-Screen) DI MI FTAHUL HUDA KEPANJEN
Authors: Ulfa Nanik , Firdausi Rofiqoh 
Published: October 2024

Wabah Covid-19 merupakan bencana dunia yang juga melanda negara Indonesia, segala aspek kehidupan menjadi terdampak akibat pandemi covid-19, begitu juga dengan dunia pendidikan. Adapun keputusan pemerintah melalui SK Mendikbud No.36962 yang memutuskan bahwa pembelajaran sekolah dilaksanakan secara Daring. Berdasarkan pertimbangan tersebut diatas, maka pembelajaran daring merupakan solusi yang tepat dalam pendidikan, mengingat kondisi anak-anak sangat rentan terpapar virus. Namun solusi tersebut juga menyisakan masalah dalam pelaksanaan pembelajaran, oleh sebab itu sangat diperlukan sebuah peningkatan kemampuan dalam berinovasi untuk pelaksanaan pembelajaran. PKM dilaksanakan di MI Miftahul Huda Desa Mojosari Kecamatan Kepanjen Kabupaten Malang, hal ini berdasarkan atas kerjasama Kepala Madrasah dan Tim UNIRA. Adapaun pelatihan dengan materi pemanfaatan aplikasi AZ-Screen untuk pembelajaran Daring. Pelaksanaan kegiatan selama dua hari, yaitu pada tanggal 4 samapai 5 januari 2020. Adapun hasil dari kegiatan tersebut adalah video pemaparan materi yang dapat digunakan oleh guru dalam pembelajaran Daring. Setelah pelaksanaan PKM diketahui bahwa 90% materi dan praktek dapat dikuasai oleh peserta.
Perancangan Sistem Pakar Diagnosis Penyakit Hepatitis Berbasis Website Dengan Metode Naive Bayes (Studi Kasus Insitut Shanti Bhuana)
Authors: Deli Anggelia , Gudiato Candra 
Published: October 2024

Penyakit Hepatitis merupakan penyakit yang sangat berbahaya bagi manusia dan dapat menyebabkan kematian. Diagnosis awal penyakit hepatitis ini sangat penting dilakukan agar dapat segera ditangani dan diobati. Terdapat 5 jenis virus hepatitis, yaitu Virus hepatitis A (VHA), Virus Hepatitis B (VHB), Virus Hepatitis C (VHC), Virus Hepatitis D (VHD),dan Virus Hepatitis E (VHE). Pada kampus Institut Shanti Bhuana, mahasiswa yang baru masuk melakukan tes hepatitis, karena mahasiswa yang baru masuk wajib tinggal di asrama selama 2 tahun. Karena dalam asrama hidup bersama, jadi pencegahan penyakit hepatitis ini sangat penting agar menghindari penyakit menular ini. Tujuan dari penelitian ini adalah merancang dan membangun sebuah sistem pakar yang dapat mendeteksi kemungkinan terjadinya penyakit hepatitis yang dapat digunakan oleh semua orang khususnya mahasiswa Institut Shanti Bhuana untuk memahami betapa pentingnya mengetahui arti, pencegahan, solusi dari penyakit hepatitis dan bagaimana proses pencegahannya serta untuk mendeteksi penyakit hepatitis sedini mungkin. Sistem pakar yang dirancang ini menerapkan perhitungan naïve bayes. Pada artikel ini fokus utamanya adalah perancangan agar nantinya dapat dipakai dan digunakan oleh Instiitut Shanti Bhuana.
Formulation and characterization of cholesterol-based nanoparticles of gabapentin protecting from retinal injury
Authors: Mokhtar Hatem I. , Khodeer Dina M. , Alzahrani Sharifa , Qushawy Mona , Alshaman Reem , Elsherbiny Nehal M. , Ahmed Esam Sayed , Abu El Wafa Esam Ghanem , El-Kherbetawy Mohamed K. , Gardouh Ahmed R. , Zaitone Sawsan A. 
Published: October 2024

IntroductionThis study aimed to prepare cholesterol and stearic acid-based solid lipid nanoparticles of gabapentin (GAB-SLNs) for protection against streptozotocin (STZ)-induced retinal injury in rats.MethodsWe prepared four preparations of GAB-SLNs using a hot high-shear homogenization ultrasonication process, and the best formulation was selected and tested for biological activity. The retinal injury was brought in male adult albino rats while gabapentin doses continued for 6 weeks. Six groups of rats were assigned as the vehicle, diabetic, diabetic + gabapentin (10–20 mg/kg), and diabetic + GAB-SLNs (10–20 mg/kg). GAB-SLN#2 was selected as the optimized formulation with high entrapment efficacy (EE%, 98.64% ± 1.97%), small particle size (185.65 ± 2.41 nm), high negative Zeta potential (−32.18 ± 0.98 mV), low polydispersity index (0.28 ± 0.02), and elevated drug release (99.27% ± 3.48%). The TEM image of GAB-SLN#2 revealed a smooth surface with a spherical shape.ResultsGAB-SLNs provided greater protection against retinal injury than free gabapentin as indicated by the histopathology data which demonstrated more organization of retinal layers and less degeneration in ganglion cell layer in rats treated with GAB-SLN#2. Further, GAB-SLN#2 reduced the inflammatory proteins (IL-6/JAK2/STAT3) and vascular endothelial growth factor (VEGF).ConclusionThe preparation of GAB-SLNs enhanced the physical properties of gabapentin and improved its biological activity as a neuroprotectant. Further studies are warranted to validate this technique for the use of oral gabapentin in other neurological disorders.
Comparing the frequency, antifungal susceptibility, and enzymatic profiles of the oral fungal composition in patients with and without Alzheimer's disease admitted to a neurology clinic
Authors: Golipoor Mandana , Rafat Zahra , Saberi Alia , Roostaei Davoud , Shabanpour Amir-Mohammad 
Published: October 2024

BackgroundStudies have shown that changes in the frequency of oral microorganisms may play a key role in the development of Alzheimer's disease (AD). However, no research has been conducted on the oral fungal composition in AD-patients. The present study aimed to investigate the changes in the frequency of oral fungal composition, the antifungal susceptibility, and the enzymatic profiles of oral fungal composition in patients suffering from AD compared to non-AD individuals.Materials and methodsIn the present analytical cross-sectional study during 12 months, 76 hospitalized patients with AD were matched with 76 individuals without AD. A sterile serum physiology-moistened cotton-tipped swab was used to sample the mouth area. All swabs were cultured on Sabouraud Chloramphenicol Agar. Fungal identified were confirmed through the PCR-sequencing techniques. Enzyme activity index (EAI) for important pathogenic factors including proteinase, esterase and hemolysin was measured using relevant protocols. The susceptibility to 8 antifungal agents (nystatin, voriconazole, itraconazole, fluconazole, posaconazole, amphotericin B, 5-fluorocytosine, and caspofungin) against fungal strains obtained from AD-patients was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, document M38-A2 for filamentous fungi, and document M27-A4 for yeasts.ResultsThe results showed that compared to the non-AD individuals, the prevalence of oral fungal composition in AD group was 1.6 times higher. Candida albicans was the most common fungal species isolated from oral swab samples of AD group (n=53, 80%) and non-AD group (n=28, 40%), and the diversity of the oral fungal composition in AD-patients were lower than non-AD individuals. Among the 3 investigated virulence factors, a statistically significant difference was shown in terms of hemolysin activity level between the two studied groups (p&lt;0.05) and the activity level of esterase and proteinase enzymes did not show a significant difference in the two studied groups (p&gt;0.05). The results showed that almost all of the tested isolates were susceptible to nystatin, the most widely prescribed antifungal to treat superficial infections, and only 1.69 % (2/118) of the Candida isolates were resistant to this antifungal drug.ConclusionUnderstanding the changes in the frequency of oral fungal composition the antifungal susceptibility, and the enzymatic profiles of oral fungal composition in patients suffering from AD compared to non-AD individuals makes it possible to better understand the etiology of this disease.
Different implications of daytime and nighttime heart rate variability on total burden of cerebral small vascular disease in patients with nondisabling ischemic cerebrovascular events
Authors: Zhang Zhixiang , Lv Yijun , Wang Qian , Wang Yan , Zhang Min , Cao Yongjun 
Published: October 2024

ObjectiveThis study aimed to explore the relationship between total burden of cerebral small vessel disease (CSVD) and daytime and nighttime heart rate variability (HRV) parameters.MethodConsecutive patients with nondisabling ischemic cerebrovascular events were recruited from the cerebrovascular disease clinic of Changzhou Second People's Hospital between January 2022 and June 2023. A total of 144 enrolled participants were divided into a mild CSVD group (74 patients) and a moderate-to-severe CSVD group (70 patients) based on total burden of CSVD. Various HRV parameters measured during 24-h, 4-h daytime, and 4-h nighttime periods (including natural log–transformed [ln] root mean square of successive RR interval differences [RMSSD], ln absolute power of the high-frequency band [0.15–0.4 Hz] [HF], ln absolute power of the low-frequency band [0.04–0.15 Hz][LF], and LF-to-HF ratio [LF/HF]) were then assessed in the 2 groups. Spearman correlation analysis was used to assess the correlation between total burden of CSVD and HRV parameters. HRV parameters with P-value &lt; 0.05 in correlation analysis were included in the multivariable logistic regression analysis, and restricted cubic spline analysis was performed to assess dose-response relationships.ResultsDaytime 4-h lnRMSSD (r = –0.221; P = 0.008) and 4-h lnHF (r = –0.232; P = 0.005) were negatively correlated with total burden of CSVD, and daytime 4-h lnLF/HF (r = 0.187; P = 0.025) was positively correlated with total burden of CSVD. There was no correlation between nighttime HRV parameters and total burden of CSVD. After adjustments were made for potential confounders, daytime 4-h lnRMSSD (OR = 0.34; 95% CI: 0.16–0.76), 4-h lnHF (OR = 0.57; 95% CI: 0.39–0.84), and 4-h lnLF/HF (OR = 2.12; 95% CI: 1.18–3.82) were independent predictors of total burden of CSVD (all P &lt; 0.05). S-shaped linear associations with moderate-to-severe total burden of CSVD were seen for daytime 4h-lnRMSSD (P for nonlinearity = 0.543), 4-h lnHF (P for nonlinearity = 0.31), and 4-h lnLF/HF (P for nonlinearity = 0.502).ConclusionDaytime parasympathetic HRV parameters are independent influencing factors of total burden of CSVD and may serve as potential therapeutic observation indicators for CSVD.
Association between dietary vitamin E and osteoporosis in older adults in the United States
Authors: Zhuang Ruoyu , Hou Wei , Zhang Ting , Wang Tao 
Published: October 2024

BackgroundIncreased oxidative stress due to aging can lead to increased bone loss. The most abundant form of vitamin E, namely α-tocopherol, has high antioxidant properties and biological activity; however, its effect on osteoporosis has not been well studied in humans. We aimed to investigate the association between dietary vitamin E (α-tocopherol) and osteoporosis among older adults in the United States.MethodsThis cross-sectional study analyzed data on older adults in the United States aged ≥50 years from the 2007–2010, 2013–2014 and 2017–2020 pre-pandemic cycles of the National Health and Nutrition Examination Survey. Sample-weighted multivariate regression models were used, with adjustments for relevant confounders.ResultsThis study comprised 5,800 individuals with available data on dietary intake and bone mineral density of hip and spine. The mean participant age was 61.4 (standard deviation, 8.7) years, and approximately 9.9% had osteoporosis. High vitamin E intake was significantly associated with a reduced risk of osteoporosis (odds ratio, 0.96, 95% confidence interval, 0.93–0.98). In addition, there was evidence of interaction between dietary vitamin E and prior fracture on preventing osteoporosis.ConclusionsOur study indicated a linear association between dietary vitamin E levels and osteoporosis in an older population in the United States. Further research is required to explore the potential effects of different forms of vitamin E on osteoporosis.