bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long noncoding RNA BDNF‐AS inversely regulated BDNF and modulated high‐glucose induced apoptosis in human retinal pigment epithelial cells
Authors: Li Yanyan , Xu Feng , Xiao Hanyan , Han Feng 
Published: June 2017

AbstractIn this study, we characterized the functional role of long noncoding RNA (lncRNA), brain derived neurotrophic factor anti‐sense (BDNF‐AS) in regulating D‐glucose‐induced (DGI) apoptosis in human retinal pigment epithelial (RPE) cells. Human RPE cell line, ARPE‐19 cells were cultured in vitro and treated with various concentrations of D‐glucose for 24 h. A TUNEL assay was applied with immunohistochemical and quantitative approaches to assess the apoptotic effect of D‐glucose. Under the condition of 50 mM D‐glucose, qPCR was used to assess gene expression of BDNF and BDNF‐AS in ARPE‐19 cells. Using siRNA transfection, BDNF‐AS was endogenously knocked down in ARPE‐19 cells. The effects of BDNF‐AS downregulation on DGI apoptosis and BDNF expression were assessed by TUNEL assay, qPCR, and Western blot, respectively. Furthermore, in BDNF‐AS‐downregulated ARPE‐19 cells, secondary siRNA transfection was conducted to knock down endogenous BDNF expression. Its effect on BDNF‐AS‐associated apoptotic regulation was further evaluated. High concentrations of D‐glucose induced significant apoptosis in ARPE‐19 cells in vitro. With treatment of 50 mM D‐glucose, BDNF was markedly downregulated whereas BDNF‐AS upregulated in ARPE‐19 cells. SiRNA‐mediated BDNF‐AS downregulation ameliorated DGI apoptosis and upregulated BDNF in ARPE‐19 cells. In addition, inhibiting BDNF reversed the protective effect of BDNF‐AS downregulation on DGI apoptosis. Our results suggest that BDNF‐AS, through inverse regulation of BDNF, might play a critical role in the process of DGI apoptosis in diabetic retinopathy.
Obacunone causes sustained expression of MKP‐1 thus inactivating p38 MAPK to suppress pro‐inflammatory mediators through intracellular MIF
Authors: Gao Yuan , Hou Rui , Liu Fen , Liu Haibo , Fei Qiaoling , Han Yixin , Cai Runlan , Peng Cheng , Qi Yun 
Published: June 2017

AbstractObacunone (OBA) is a highly oxygenated triterpenoid with various pharmacological activities. In this study, we explored its anti‐inflammatory effect and underlying mechanisms in LPS‐activated macrophages. Our data showed that OBA potently decreased pro‐inflammatory mediators (eg, NO, IL‐6, IL‐1β, and MCP‐1) at the transcriptional and translational levels without cytotoxicity. A mechanism study showed that OBA significantly suppressed p38‐mediated AP‐1 signaling by stabilizing the mRNA of mitogen‐activated protein kinase phosphatase 1 (MKP‐1), thus prolonging the expression time of the MKP‐1 protein. Next, we used computational target‐fishing technology to predict the possible target of OBA. Only one potential target, macrophage migration inhibitory factor (MIF), was presented. Experimentally, the interaction between OBA and MIF was also confirmed. By using an anti‐mouse MIF antibody, extracellular MIF (exMIF) was neutralized. Our results showed that autocrine MIF had slight influence on the pro‐inflammatory mediator production. Correspondingly, the anti‐inflammatory activity of OBA was also not affected. Accordingly, we knocked down the MIF gene in RAW 264.7 cells and obtained stable MIF deficient cells MIF(−), in which the effects of OBA on p38 phosphorylation, AP‐1 activation, and pro‐inflammatory mediator production in response to LPS nearly disappeared. In contrast to MIF(+) cells, the MKP‐1 protein expression time of the MIF(−) cells was markedly prolonged. We conclude that OBA exerts its anti‐inflammatory effect by targeting intracellular MIF (inMIF) inhibition to regulate the MKP‐1/p38/AP‐1 pathway. Our findings also provide a chain of evidence that the inhibition of inMIF, rather than exMIF, may become a novel target for inflammation.
Diagnostic reliability of Architect anti‐HCV assay: Experience of a tertiary care hospital in India
Authors: Fletcher Gnanadurai John , Raghavendran Anantharam , Sivakumar Jayashree , Samuel Prasanna , Abraham Priya 
Published: June 2017

Background & AimsAnti‐HCV assays are prone to false positive results. Thus, accurate detection of HCV infection is critical for the timely therapeutic management. This study ascertained the reliability of Architect anti‐HCV assay (Abbott) and to estimate the agreement of this assay with Ortho HCV 3.0 ELISA Test System with Enhanced SAVe (Ortho), HCV Tri‐dot (Tri‐dot) and HCV‐PCR in a tertiary care setting.MethodsA total of 78 788 consecutive sera were routinely screened for anti‐HCV antibodies using Architect. All repeatedly reactive anti‐HCV sera (n=1000) and anti‐HCV negative sera (n=300) were tested in Ortho and in Tri‐dot assays. Representative proportions of sera (n=500) with various signal‐to‐cut‐off (S/Co) ratio were also compared with HCV‐PCR.ResultsWhen Architect was compared with Ortho, Tri‐dot, and HCV‐PCR, the level of agreement as assessed by kappa were .26, .16, and .27 respectively. Using Latent class analysis (LCA), we found that sensitivity and specificity were 100% and 36.1% for Architect, 93.8% and 100% for Ortho and 63.8% and 100% for Tri‐dot respectively. The median S/CO ratio of Architect and Ortho anti‐HCV assays were significantly different between HCV‐PCR positive and negative results (P<.0001). Furthermore, Architect S/CO ratio of >8 showed higher accuracy indices in both anti‐HCV assays.ConclusionsArchitect can be used as a screening assay because of its high sensitivity, high throughput, and short turnaround time. However, S/Co ratios of ≥1 to <8 in Architect necessitates HCV PCR to identify current infection and or EIA to distinguish true positivity from false biological positivity.
The advantages of live/real‐time three‐dimensional echocardiograhy in the assessment of left ventricular myxoma, which causes partial left ventricular outflow tract obstruction
Authors: Kalkan Ali Kemal , Uygur Begum , Karakayalı Muammer , Kadirogulları Ersin , Erturk Mehmet 
Published: June 2017

ABSTRACTMyxomas are the most common cardiac primary tumors; however, left ventricular myxomas are extremely rare. We describe a young female patient with a giant left ventricular myxoma causing partial obstruction of the left ventricular outflow tract, who underwent successful surgical resection. Real‐time three‐dimensional echocardiography yielded incremental value to two‐dimensional echocardiography by allowing better assess of the true size, extent, attachment, and morphology of the tumor. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 46:273–277, 2018
Early Activation of MAPK and Apoptosis in Nutritive Embryos of Calyptraeid Gastropods
Authors: Lesoway Maryna P. , Collin Rachel , Abouheif Ehab 
Published: June 2017

ABSTRACTInvestigation of alternative phenotypes, different morphologies produced by a single genome, has contributed novel insights into development and evolution. Yet, the mechanisms underlying developmental switch points between alternative phenotypes remain poorly understood. The calyptraeid snailsCrepidula navicellaandCalyptraea lichenproduce two phenotypes: viable and nutritive embryos, where nutritive embryos arrest their development after gastrulation and are ingested by their viable siblings as a form of intracapsular nutrition. Here, we investigate the activity of mitogen‐activated protein kinase (MAPK, ERK1/2) and apoptosis during early cleavage. MAPK and apoptosis, found in a previous transcriptomic study, are known to be involved in organization of other spiralian embryos and nutritive embryo development, respectively. In the modelCrepidula fornicata, MAPK activation begins at the 16‐cell stage. In contrast, we discovered inC. navicellaandC. lichenthat many embryos begin MAPK activation at the one‐cell stage. A subset of embryos shows a similar pattern of MAPK activation toC. fornicataat later stages. In all stages where MAPK is detected, the activation pattern is highly variable, frequently occurring in all quadrants or in multiple tiers of cells. We also detected apoptosis in cleaving embryos, whileC. fornicataandCrepidula lessoni, which do not produce nutritive embryos, show no signs of apoptosis during cleavage. Our results show that MAPK and apoptosis are expressed during early development in species with nutritive embryos, and raises the possibility that these processes may play a role and even interact with one another in producing the nutritive embryo phenotype.
Sustained secretion of anti‐tumor necrosis factor α monoclonal antibody from ex vivo genetically engineered dermal tissue demonstrates therapeutic activity in mouse model of rheumatoid arthritis
Authors: Zafir‐Lavie Inbal , Miari Reem , Sherbo Shay , Krispel Simi , Tal Osnat , Liran Atar , Shatil Tamar , Badinter Felix , Goltsman Haim , Shapir Nir , Benhar Itai , Neil Garry A. , Panet Amos 
Published: June 2017

AbstractBackgroundRheumatoid arthritis (RA) is a symmetric inflammatory polyarthritis associated with high concentrations of pro‐inflammatory, cytokines including tumor necrosis factor (TNF)‐α. Adalimumab is a monoclonal antibody (mAb) that binds TNF‐α, and is widely used to treat RA. Despite its proven clinical efficacy, adalimumab and other therapeutic mAbs have disadvantages, including the requirement for repeated bolus injections and the appearance of treatment limiting anti‐drug antibodies. To address these issues, we have developed an innovative ex vivo gene therapy approach, termed transduced autologous restorative gene therapy (TARGT), to produce and secrete adalimumab for the treatment of RA.MethodsHelper‐dependent (HD) adenovirus vector containing adalimumab light and heavy chain coding sequences was used to transduce microdermal tissues and cells of human and mouse origin ex vivo, rendering sustained secretion of active adalimumab. The genetically engineered tissues were subsequently implanted in a mouse model of RA.ResultsTransduced human microdermal tissues implanted in SCID mice demonstrated 49 days of secretion of active adalimumab in the blood, at levels of tens of microgram per milliliter. In addition, transduced autologous dermal cells were implanted in the RA mouse model and demonstrated statistically significant amelioration in RA symptoms compared to naïve cell implantation and were similar to recombinant adalimumab bolus injections.ConclusionsThe results of the present study report microdermal tissues engineered to secrete active adalimumab as a proof of concept for sustained secretion of antibody from the novel ex vivo gene therapy TARGT platform. This technology may now be applied to a range of antibodies for the therapy of other diseases.
Making sense of organizational change: Is hindsight really 20/20?
Authors: Gover Laura , Duxbury Linda 
Published: June 2017

SummaryThis qualitative study explores the conceptual links between 2 different approaches to managerial cognition, sensemaking and cognitive bias, in the context of organizational change. A longitudinal case study utilizing both real‐time assessments and retrospective sensemaking data from interviews with 26 hospital employees at 3 points in time was undertaken. Patterns related to individuals' retrospective accounts and real‐time assessments were identified and used to construct 4 prototypical narratives. Data analysis revealed that organizational change was not a markedly negative experience for most informants, which is contrary to the prevailing theme in the literature. This and other findings are discussed in terms of sensemaking and cognitive bias. This study makes 2 contributions to our understanding of how individual's experience and make sense of organizational change over time as (a) little is known about how the process of change unfolds over time at the individual level and (b) extant research has not investigated the extent to which individuals' retrospective sensemaking about organizational change reflects or diverges from their real‐time assessments over the course of the change. More broadly, the study provides insights and focused advice for management researchers regarding the use of retrospective data to understand individuals' perceptions of situations that have already occurred.
Does homesickness undermine the potential of job resources? A perspective from the work–home resources model
Authors: Du Danyang , Derks Daantje , Bakker Arnold B. , Lu Chang‐qin 
Published: June 2017

SummaryRapid economic development in recent decades has resulted in a considerable increase in the number of people working far away from their home locations. Homesickness is a common reaction to the separation from home. Our research uses the work–home resources model to explain how the experience of homesickness can undermine the positive effect of job resources on job performance (i.e., task performance and safety behavior). In addition, we hypothesize that emotional stability and openness are key resources that can buffer the negative interference of homesickness with the job resources–performance relationship. We conducted two studies to test our hypotheses. Study 1 was a two‐wave longitudinal study using a migrant manufacturing worker sample. In this study, homesickness was measured at the between‐person level, and performance was measured three months later. Study 2 was a daily diary study conducted in a military trainee sample. In this study, homesickness was measured at the within‐person level to capture its fluctuations over 20 days, and daily job performance was assessed using supervisor ratings. Both studies showed evidence of the hypothesized moderating effect of homesickness and three‐way interaction effects of job resources, homesickness, and key resources (i.e., emotional stability and openness) on task performance and safety behavior.
Evaluation of NCEP‐2 and CFSR reanalysis seasonal temperature data in China using detrended fluctuation analysis
Authors: Zhao Shanshan , He Wenping , Jiang Yundi 
Published: June 2017

ABSTRACTIn the present study, the performance of the National Centers for Environmental Prediction/Department of Energy (NCEP/DOE) Atmospheric Model Intercomparison Project (AMIP‐II) reanalysis (NCEP‐2) and NCEP Climate Forecast System Reanalysis (CFSR) seasonal temperature data for China was quantitatively evaluated by using detrended fluctuation analysis. The results indicate that the quality of the NCEP‐2 temperature data is the highest in autumn, while the quality of CFSR data set is the highest in summer. Both the quality of NCEP‐2 and that of CFSR temperature data is the lowest in winter. The quality is very low in eastern Tibetan Plateau and most of Xinjiang at a significance level of 0.05 for the NCEP‐2 and CFSR reanalysis temperature data including three elements: the daily average temperature (Tmean), the daily maximum temperature (Tmax) and the daily minimum temperature (Tmin). The performance of the NCEP‐2 Tmean data is poorer than those of the NCEP‐2 Tmax and Tmin data for all four seasons, while the performance of CFSR Tmean is better than that of CFSR Tmax and Tmin for all four seasons. In addition, the NCEP‐2 Tmax, Tmin and CFSR Tmean, Tmax data perform well in most of central and eastern China for all four seasons. The quality of the CFSR Tmean is better than that of the NCEP‐2 Tmean for all four seasons. However, the NCEP‐2 Tmax and Tmin data have higher credibility than that of the CFSR Tmax and Tmin data for all four seasons. Therefore, it is important to consider the reliability of the NCEP‐2 and CFSR daily temperature reanalysis data in different districts and seasons when drawing conclusions from the different reanalysis data.
Synoptic conditions leading to extremely warm periods in Western Iran
Authors: Darand M. , Garcia‐Herrera R. , Asakereh H. , Amiri R. , Barriopedro David 
Published: June 2017

ABSTRACTThe purpose of this study is the characterization of the synoptic circulation patterns leading to extremely warm periods (EWPs) over the Kurdistan province of Iran. Daily maximum temperature data from 27 meteorological stations were used for the period 1962–2012. Data were interpolated to a 6 × 6 km grid using the geospatial Kriging method. EWPs were defined as a period of at least 6 consecutive days with maximum temperature above the local daily‐based 95th percentile of the 1971–2000 period, with additional criteria for spatial extension. The identified EWPs are distributed through the whole year, with maximum frequencies in winter and summer. Interestingly, the decade 1988–1997 did not record any extreme episode and summer extremes were recorded only before 1983. The associated synoptic patterns were classified into four clusters by applying the Ward's hierarchical clustering method to sea level pressure and Z500 data. Four clusters are identified: two represent warm periods in winter, one those occurring spring, especially in March, and another one mainly reflecting summer heat waves. In general, it is found that EWPs are associated with statistically significant anomalies in regional circulation. A deep trough of geopotential height in 500 hPa over North Africa and a ridge directly over the study area were the more important causes for the occurrence of EWPs. Other synoptic configurations that favour extreme periods in winter and summer include, respectively, the northward expansion of the Sudan low pressure with an associated southerly wind advection and the persistence of anticyclonic circulation at all tropospheric levels enhancing subsidence and adiabatic heating. The spring pattern is the most frequent one (nearly 48% of all cases) and reflects a southeastward extension of the subpolar low pressure that leads to the displacement of the Siberian high to south and southwestern Asia and the accompanied northward expansion of the Sudan low.
Assessing the climatic and environmental impacts of mid‐tropospheric anticyclones over Alaska
Authors: McLeod Jordan T. , Ballinger Thomas J. , Mote Thomas L. 
Published: June 2017

ABSTRACTOngoing climatic and cryospheric changes observed throughout the greater Alaska region are interconnected and often linked to oceanic and atmospheric patterns and processes that operate on varying spatiotemporal scales. To evaluate the long‐term, mid‐tropospheric circulation field across Alaska, and possible connections to climate and environmental change in the Pacific sector of the Arctic, the Alaska Blocking Index (ABI) is developed over the domain (54°–76°N, 125°–180°W) using daily gridded 500 hPa geopotential height fields derived from the ERA‐40 (1958–1978) and ERA‐Interim (1979–2014) data sets, 1958–2014. Climatological characteristics of the seasonal and annual ABI conditions are evaluated and periods of prevalent blocking conditions are identified and subsequently linked to possible local and large‐scale forcing mechanisms. The ABI has exhibited positive trends during all seasons and annually since 1979. Many of the extreme high ABI values occurred since 2000, including the highest annual values in 2013 and 2014. Anomalous blocking patterns in all seasons are associated with diminished snow depth and sea‐ice cover, positive near‐surface air temperature anomalies, and anticyclonic flows of heat and moisture across the domain. The ABI is also shown to differ from the long‐term variability and atmospheric circulation responses associated with phases of the Pacific–North American pattern and Pacific Decadal Oscillation, revealing some notable spatial and temporal disconnects between the region‐centric, high‐latitude blocking flow and some of the predominant modes of sea surface temperature and middle tropospheric circulation variability in the Northern Hemisphere.
Line‐time optimization technology for ultra‐large and high‐resolution liquid crystal displays
Authors: Lee Seung‐Hyuck , Jeon Dong‐Hwan , Kim Jong‐Man , Kim Minkoo , Yu Byung‐Chang , Lee Seung‐Woo 
Published: June 2017

AbstractThis paper proposes a line‐time optimization (LTO) technology for ultra‐large and high‐resolution liquid crystal display (LCD) televisions. Line‐time optimization enables a single‐bank data driver configuration without severe image degradation. When the proposed method is applied to an ultra‐high‐definition (UHD) LCD with a single‐bank data driver scheme, the LCD performance comparable to that of a dual‐bank data driver method can be obtained. The implementation of the proposed method helps in achieving desirable goals such as a reduction in the number of drivers and realization of a much more flexible design of UHD LCDs.
Comparative analysis of main bio‐active components in the herb pair Danshen‐Honghua and its single herbs by ultra‐high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry
Authors: Qu Cheng , Pu Zong‐Jin , Zhou Gui‐Sheng , Wang Jun , Zhu Zhen‐Hua , Yue Shi‐Jun , Li Jian‐Ping , Shang Li‐Li , Tang Yu‐Ping , Shi Xu‐Qin , Liu Pei , Guo Jian‐Ming , Sun Jing , Tang Zhi‐Shu , Zhao Jing , Zhao Bu‐Chang , Duan Jin‐Ao 
Published: June 2017

AbstractA sensitive, reliable, and powerful ultra‐high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method was developed for simultaneous quantification of the 15 main bio‐active components including phenolic acids and flavonoids within 13 min for the first time. The proposed method was first reported and validated by good linearity (r2 > 0.9975), limit of detection (1.12–7.01 ng/mL), limit of quantification (3.73–23.37 ng/mL), intra‐ and inter‐day precisions (RSD ≤ 1.92%, RSD ≤ 2.45%), stability (RSD ≤ 5.63%), repeatability (RSD ≤ 4.34%), recovery (96.84–102.12%), and matrix effects (0.92–1.02). The established analytical methodology was successfully applied to comparative analysis of main bio‐active components in the herb pair Danshen‐Honghua and its single herbs. Compared to the single herb, the content of most flavonoid glycosides was remarkably increased in their herb pair, and main phenolic acids were decreased, conversely. The content changes of the main components in the herb pair supported the synergistic effects on promoting blood circulation and removing blood stasis. The results provide a scientific basis and reference for the quality control of Danshen‐Honghua herb pair and the drug interactions based on variation of bio‐active components in herb pairs.