bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of paratracheal lymph nodes in laryngeal cancer with subglottic involvement
Authors: Lucioni Marco , D'Ascanio Luca , De Nardi Elena , Lionello Marco , Bertolin Andy , Rizzotto Giuseppe 
Published: September 2017

AbstractBackgroundThe purpose of this study was to present our findings on the involvement of paratracheal lymph nodes in laryngeal squamous cell carcinoma (SCC) with subglottis extension, which is controversial.MethodsWe assessed 196 patients with laryngeal SCC involving the subglottis, treated with open laryngeal surgery with or without paratracheal neck dissection (PTND). The relationship of the paratracheal lymph node metastatic pattern with laterocervical nodal status and tumor location within different subglottic subsites was analyzed. The influence of PTND on regional disease control was assessed.ResultsParatracheal lymph nodes were affected in 12.2% of cases. An increased frequency (P = .064) of paratracheal metastasis was noticed in case of anterior subglottis extension with respect to other subsites. A correlation (P < .001) between paratracheal lymph node and laterocervical node involvement was found among subjects with posterior subglottic extension.ConclusionsProphylactic PTND is indicated in laryngeal SCC with anterior subglottic extension and/or posterior subglottis involvement with clinically apparent laterocervical node metastases.
Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey
Authors: Koçyiğit Ümit M. , Durna Daştan Sevgi , Taslimi Parham , Daştan Taner , Gülçin İlhami 
Published: September 2017

AbstractIn this study, carbonic anhydrase (CA) enzyme was purified and characterized from blood samples of Kangal Akkaraman sheep and inhibitory properties on certain antibiotics were examined. CA purification was composed of preparation of the hemolysate and conducting the Sepharose‐4B‐tyrosine‐sulfanilamide affinity gel chromatography in having specific activity of 11626 EU mg−1, yield of 14.40%, and 242.76‐fold purification. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis was performed to assess the enzyme purity and a single band was observed. Some antibiotics were exhibited in vitro inhibition on the CA activity. IC50 values of these inhibitors were calculated by plotting activity percentage. IC50 values of certain drugs (dexamethasone; caffeine; metamizole sodium; tetramisol; ceftiofur HCl; ivermectin; tavilin 50; penokain G; neosym; and sulfamezathine) were found as 0.38, 8.24, 285.53, 114.77, 5.33, 2.76, 27.58, 213.50, 208.28, and 36.60 μM, respectively. Ki values of different drugs on Kangal Akkaraman sheep blood CA activity were found in the range of 0.21 ± 0.038–266.64 ± 37.11 μM.
An immobilized and highly stabilized self‐sufficient monooxygenase as biocatalyst for oxidative biotransformations
Authors: Valencia Daniela , Guillén Marina , Fürst Maximilian JLJ , López‐Santín Josep , Álvaro Gregorio 
Published: September 2017

AbstractBACKGROUNDThe requirement for expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self‐sufficient Baeyer–Villiger monooxygenases is discussed.RESULTSA bifunctional biocatalyst composed of an NADPH‐dependent cyclohexanone monooxygenase (CHMO) fused to an NADP+‐accepting phosphite dehydrogenase (PTDH) catalyzes ϵ‐caprolactone synthesis from cyclohexanone, using phosphite as a cheap sacrificial substrate for cofactor regeneration. Several immobilized derivatives of the fused enzyme have been prepared with high immobilization yield (97%); the one obtained by affinity adsorption on Co‐IDA (Co: cobalt chelated; IDA: iminodiacetic acid) support has shown to be highly stable affording average yields of 80% after 18 reaction cycles.CONCLUSIONSThe immobilized self‐sufficient monooxygenase has demonstrated to be able to perform Baeyer–Villiger oxidation with efficient cofactor recovery and biocatalyst recycling. The proposed biocatalytic process offers access to valuable molecules with high atom economy and limited waste generation. © 2017 Society of Chemical Industry
Simultaneous removal of selenite and phenol from wastewater in an upflow fungal pellet bioreactor
Authors: Werkneh Adhena A , Rene Eldon R , Lens Piet NL 
Published: September 2017

AbstractBACKGROUNDThe simultaneous treatment of wastewater containing selenite and phenol in batch and continuous systems, inoculated with pellets of Phanerochaete chrysosporium, was evaluated in this study.RESULTSSynthetic oil refinery wastewater containing 15 mg L−1 selenite and 100–600 mg L−1 phenol was used in batch incubations for 5 days. Orange‐red colored pellets developed during selenite incubations in the presence of up to 400 mg L−1 of phenol, which confirms the formation of elemental selenium with removal efficiencies ranging from 57–78.5% and 75–90.8% for, respectively, phenol and selenite. Continuous experiments in two upflow fungal pelleted bioreactors (30 °C, pH 4.5, hydraulic retention time of 16.7 h) fed the synthetic oil refinery wastewater containing phenol or phenol with selenite were performed for 38 days. The maximum removal efficiency of phenol in the fungal bioreactor was 100% (up to day 26).CONCLUSIONSelenite mass balance showed that the overall maximum removal efficiency of selenite in the presence of phenol was 67.3%. The critical phenol and selenite loading rates of the upflow bioreactors were ∼12 and 4.3 mg L−1 h−1, respectively. © 2017 Society of Chemical Industry
Evaluation of a low‐cost magnesium product for phosphorus recovery by struvite crystallization
Authors: Crutchik Dafne , Rodrigues Samuel , Ruddle David , Garrido Juan Manuel 
Published: September 2017

AbstractBackgroundThe development of a cost‐effective process of struvite crystallization requires the selection of appropriate sources of alkali and magnesium. In this study, the effectiveness of two industrial grade products, MgO and Mg(OH)2, as magnesium and alkali sources to recover phosphorus as struvite were investigated and compared in a first set of experiments. Subsequently, the use of industrial Mg(OH)2 was compared in two different struvite crystallization systems, an upflow fluidized bed reactor (FBR) and a continuous stirred tank reactor (CSTR) coupled to a settler tank.ResultsAt the same operational conditions, the consumption of MgO was higher than Mg(OH)2 consumption. Moreover, industrial Mg(OH)2 consumption for FBR and the CSTR operation was 1.6 and 1.1 1 mol Mg added mol‐1 P precipitated, respectively. This difference was caused by the high mixing intensity and the higher contact time between the Mg(OH)2 slurry and the influent in the CSTR, favouring the conversion.ConclusionsBoth industrial grade magnesium products are promising options for struvite crystallization. However, Mg(OH)2 was more effective than the starting material, MgO, to recover phosphorus. Struvite crystallization by adding an industrial grade Mg(OH)2 could be economically viable with regard to alternative physico‐chemical P removal processes using metal salts, increasing the attractiveness of this P recovery process. © 2017 Society of Chemical Industry
In situ purification of periplasmatic L‐asparaginase by aqueous two phase systems with ionic liquids (ILs) as adjuvants
Authors: Santos João HPM , Flores‐Santos Juan C , Meneguetti Giovanna P , Rangel‐Yagui Carlota O , Coutinho João AP , Vitolo Michele , Ventura Sónia PM , Pessoa Adalberto 
Published: September 2017

AbstractBACKGROUNDL‐asparaginase (ASNase) is an important biopharmaceutical used to treat the acute lymphoblastic leukemia (ALL) and lymphosarcoma. Considering its main use in cancer therapy, the most important request for ASNase production is the need for a highly pure biopharmaceutical obtained in the final of the downstream process, which is considered as the crucial step in its production.RESULTSThis work proposes the use of polymer–salt aqueous two‐phase systems (ATPS) based on polyethylene glycol and citrate buffer, with ionic liquids (ILs) as adjuvants, combined with the permeabilization of cell membrane using n‐dodecane and glycine for the in situ purification of periplasmatic ASNase from Escherichia coli cells. The process proposed was optimized (polymer molecular weight, pH, tie‐line length/mixture point, presence, nature and concentration of the adjuvant). The results show that ASNase partitions mostly to the PEG‐rich phase, due to hydrophobic interactions between both PEG and enzyme. Remarkably, the addition of 5 wt% of 1‐butyl‐3‐methylimidazolium methanesulfonate [C4mim][CH3SO3] as adjuvant lead to high recoveries [87.94 ± 0.03 (%)], purification factors (20.09 ± 0.35), and a final specific activity SA = 3.61 ± 0.38 U mg‐1 protein, from a crude enzyme extract with a SA = 0.18 ± 0.05 U mg‐1 protein. Moreover, better results were achieved when a prepurification step consisting of an ammonium sulfate precipitation was combined with the optimized ATPS, achieving an increased SA = 22.01 ± 1.36 U mg‐1 protein and PF = 173.8.CONCLUSIONSA novel integrated downstream process was successfully implemented for the in situ purification of ASNase from fermentation broth. © 2017 Society of Chemical Industry
Prediction of overall glucose yield in hydrolysis of pretreated sugarcane bagasse using a single artificial neural network: good insight for process development
Authors: Plazas Tovar Laura , Ccopa Rivera Elmer , Pinto Mariano Adriano , Wolf Maciel Maria Regina , Maciel Filho Rubens 
Published: September 2017

AbstractBACKGROUNDIn this work a single artificial neural network (ANN) was used to model the overall yield of glucose (YGLC) as a function of a wide range of operating conditions of both pretreatment and enzymatic hydrolysis.RESULTSThe model was validated experimentally and presented good predictions of YGLC. Sensitivity analysis using the ANN model indicated that most of the operating parameters, except for pretreatment time, were statistically significant (P‐value <0.05). Experiments showed that the processing of sugarcane bagasse (in natura) results in a satisfactory glucose yield of 69.34% when pretreated for 60 min with low initial biomass concentration and acid concentration (10% and 1.0% w/v), and followed by enzymatic hydrolysis for 72 h with 3.0% w/v substrate loading and 60 FPU per gWIS enzyme concentration.CONCLUSIONThis study demonstrated how pretreatment and enzymatic hydrolysis data can be used to parameterize a single ANN model. Acceptable predictions of YGLC are achieved in terms of RSD, MSE and R2. Supported by the model, this study provided a good insight for process development. © 2017 Society of Chemical Industry
Comparison of the effects of once‐weekly and once‐daily rhPTH (1–34) injections on promoting fracture healing in rodents
Authors: Zhang Wen , Zhu Junxiong , Ma Teng , Liu Can , Hai Bao , Du Guohong , Wang Hong , Li Nan , Leng Huijie , Xu Yingsheng , Song Chunli 
Published: September 2017

ABSTRACTTo compare the efficacy of once‐weekly and once‐daily subcutaneous injections of teriparatide (recombinant human parathyroid hormone 1–34) on fracture healing, 50 adult male Sprague–Dawley rats were subjected to a unilateral tibia fracture and received internal fixation with a Kirschner needle. Based on the injection dose and frequency, the rats were randomly divided into five groups (n = 10 each): subcutaneous injections of saline or 10 µg/kg/w, 20 µg/kg/w, 10 µg/kg/d, and 20 µg/kg/d teriparatide. Four weeks later, the rats were euthanatized, and the fractured tibiae were assessed using X‐rays, dual‐energy X‐ray absorptiometry, micro‐computed tomography, the three‐point bending biomechanics test, and histology. Compared to the saline control group, either daily or weekly subcutaneous injections of teriparatide significantly increased bone mass, improved the bone microarchitecture, and promoted fracture healing (p < 0.05). There were no significant differences in bone mineral density (BMD), bone microstructure or bone strength between the 20 µg/kg/w and 10 µg/kg/d groups (p > 0.05). Teriparatide 20 µg weekly injections promoted bone fracture healing to the same extent as teriparatide 10 µg daily injections, which can dramatically decrease the cumulative dosage of teriparatide injections. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1145–1152, 2018.
Hydrogen peroxide induces programmed necrosis in rat nucleus pulposus cells through the RIP1/RIP3‐PARP‐AIF pathway
Authors: Zhao Lei , Lin Hui , Chen Songfeng , Chen Sheng , Cui Min , Shi Deyao , Wang Baichuan , Ma Kaige , Shao Zengwu 
Published: September 2017

ABSTRACTThis study aimed to systematically investigate whether programmed necrosis contributes to H2O2‐induced nucleus pulposus (NP) cells death and to further explore the underlying mechanism involved. Rat NP cells were subjected to different concentrations of H2O2 for various time periods. The cell viability was measured using a cell counting kit‐8, and the death rate was detected by Hoechst 33258/propidium iodide (PI) staining. The programmed necrosis‐related molecules receptor‐interacting protein 1 (RIP1), receptor‐interacting protein 3 (RIP3), poly (ADP‐ribose) polymerase (PARP), and apoptosis inducing factor (AIF) were determined by real‐time polymerase chain reaction and Western blotting, respectively. The morphologic and ultrastructural changes were examined by phasecontrast microscopy and transmission electron microscopy (TEM). In addition, the necroptosis inhibitor Necrostatin‐1 (Nec‐1), the PARP inhibitor diphenyl‐benzoquinone (DPQ) and small interfering RNA (siRNA) technology were used to indirectly evaluate programmed necrosis. Our results indicated that H2O2 induced necrotic morphologic and ultrastructural changes and an elevated PI positive rate in NP cells; these effects were mediated by the upregulation of RIP1 and RIP3, hyperactivation of PARP, and translocation of AIF from mitochondria to nucleus. Additionally, NP cells necrosis was significantly attenuated by Nec‐1, DPQ pretreatment and knockdown of RIP3 and AIF, while knockdown of RIP1 produced the opposite effects. In conclusion, these results suggested that under oxidative stress, RIP1/RIP3‐mediated programmed necrosis, executed through the PARP‐AIF pathway, played an important role in NP cell death. Protective strategies aiming to regulate programmed necrosis may exert a beneficial effect for NP cells survival, and ultimately retard intervertebral disc (IVD) degeneration. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1269–1282, 2018.
Analytical imaging of colour pigments used in Japanese woodblock prints using Raman microspectroscopy
Authors: Minamikawa Takeo , Nagai Daiki , Kaneko Takaaki , Taniguchi Ittetsu , Ando Mariko , Akama Ryo , Takenaka Kenji 
Published: September 2017

AbstractUkiyo‐e printing is a traditional Japanese multicolour woodblock printing style that flourished during the 18th century. Although this genre subsequently experienced a decline, interest in it has since re‐emerged. However, the materials and carving and printing technologies originally used to produce ukiyo‐e prints remain unclear due to a lack of technical information. In this study, we performed non‐invasive molecular imaging of the colour pigments in recently reproduced and original ukiyo‐e prints using Raman microspectroscopy to investigate ukiyo‐e production technologies. First, to confirm the effectiveness of this method, we obtained Raman spectral images of an ukiyo‐e print, Ohisa with a fan, that was recently reproduced using the current ukiyo‐e production protocols. The pigment species were easily identified based on their Raman spectra, and small pigment granules were observed along the paper fibres. Furthermore, we performed Raman microspectroscopy of an original ukiyo‐e print, Kabuki Actor Iwai Hanshirô VIII as Miuraya no Agemaki. Some pigment species were revealed by the Raman spectra. We also obtained high‐spatial‐resolution pigment distribution images and observed characteristic inhomogeneous colour pigment aggregations on and between the paper fibres depending on the reproduced and the original ukiyo‐e prints. Our results indicate that Raman microspectroscopy is a promising technique for determining the original ukiyo‐e print production technologies that are currently unknown.
The feasibility of a search filter for the adverse effects of nondrug interventions in MEDLINE and Embase
Authors: Golder Su , Wright Kath , Loke Yoon K. 
Published: September 2017

Authors and indexers are increasingly including terms for adverse drug effects in the titles, abstracts, or indexing of records in MEDLINE and Embase. However, it is not clear if this is the same for studies with nondrug adverse effects data.We therefore assessed the feasibility of using adverse effects terms when searching MEDLINE or Embase to retrieve papers of nondrug adverse effects.A collection of papers that reported data on nondrug adverse effects was sought from included studies of systematic reviews. Each included study was analysed to ascertain whether the corresponding record in MEDLINE and Embase included adverse effects terms in the title, abstract, or indexing.From 9129 records screened from DARE, 30 reviews evaluating nondrug adverse effects met our inclusion criteria. From these, 635 unique papers were included in our analysis.Sensitive searches for adverse effects required generic and specific named adverse effects terms using the title, abstract, and indexing. Records relating to surgical interventions were more likely to contain adverse effects terms than records relating to nonsurgical interventions. Using any adverse effects terms in the title, abstract or indexing in MEDLINE and Embase would have identified an average of 94% of papers on surgical adverse effect interventions per systematic review and 72% of papers on nonsurgical adverse effects per systematic review.Hence, while a generic nondrug adverse effect search filter may not yet be feasible, a filter for the adverse effects of surgical interventions may be within reach.
Differentiation of glycated residue numbers on heat‐induced structural changes of bovine serum albumin
Authors: Liu Jianlei , Xing Xiaojuan , Jing Hao 
Published: September 2017

AbstractBACKGROUNDGlycation is an approach in dealing with heat‐induced protein aggregation. The relationship between degree of glycation and heat‐induced structural changes is still unclear. The present work investigates the effect of different numbers and sites of glycated residues on heat‐induced structural changes of bovine serum albumin (BSA). Glycation of BSA was carried out with xylose (Xyl) and galactose (Gal) by Maillard reaction. Glycated residues in BSA were identified by liquid chromatography–tandem mass spectrometry, and heat‐induced protein structural changes were characterized by fluorescence emission and synchronous fluorescence spectra, 8‐anilino‐1‐naphthalenesulfonic acid fluorescence, Fourier transform infrared (FTIR) and circular dichroism (CD) spectra.RESULTSThe numbers of glycated residues were 2 and 13 when BSA was glycated by Gal (BSA − Gal) and Xyl (BSA − Xyl), respectively. There were shifts of maximum wavelengths and decreases in fluorescence intensities for both intrinsic and extrinsic fluorescences; shifts of FTIR amide I, III, and A bands; and decrease or increase of CD band intensities, α‐helix and β‐sheet percentages when BSA was heated. Glycation with Gal or Xyl restrained in similar degrees those changes, including fluorescence wavelengths, amide I band, CD band intensities, and α‐helix and β‐sheet percentages.CONCLUSIONXyl glycated more residues than Gal, while their effects were similar in restraining heat‐induced BSA structural changes. © 2017 Society of Chemical Industry
Uptake of heavy metals and arsenic in black soldier fly (Hermetia illucens) larvae grown on seaweed‐enriched media
Authors: Biancarosa Irene , Liland Nina S , Biemans Daan , Araujo Pedro , Bruckner Christian G , Waagbø Rune , Torstensen Bente E , Lock Erik‐Jan , Amlund Heidi 
Published: September 2017

AbstractBACKGROUNDThe black soldier fly (Hermetia illucens) is one of the most promising insect species for use in animal feed. However, studies investigating feed and food safety aspects of using black soldier fly as feed are scarce. In this study, we fed black soldier fly larvae feeding media enriched with seaweed, which contains naturally high concentrations of heavy metals and arsenic. The aim of this study was to investigate the potential transfer of such undesirable substances from the feeding media to the larvae.RESULTSThe larvae accumulated cadmium, lead, mercury and arsenic. Concentrations of these elements in the larvae increased when more seaweed was added to the feeding media. The highest retention was seen for cadmium (up to 93%) and the lowest for total arsenic (up to 22%). When seaweed inclusion exceeded 20% in the media, this resulted in larval concentrations of cadmium and total arsenic above the current European Union maximum levels for these elements in complete feed.CONCLUSIONOur results confirm that insect larvae can accumulate heavy metals and arsenic when present in the feeding media. A broader understanding of the occurrence of these undesirable substances in processed larvae products is needed to assess feed and food safety. © 2017 Society of Chemical Industry
Effects of immediate‐release niacin and dietary fatty acids on acute insulin and lipid status in individuals with metabolic syndrome
Authors: Montserrat‐de la Paz Sergio , Lopez Sergio , Bermudez Beatriz , Guerrero Juan M , Abia Rocio , Muriana Francisco JG 
Published: September 2017

AbstractBACKGROUNDThe nature of dietary fats profoundly affects postprandial hypertriglyceridemia and glucose homeostasis. Niacin is a potent lipid‐lowering agent. However, limited data exist on postprandial triglycerides and glycemic control following co‐administration of high‐fat meals with a single dose of niacin in subjects with metabolic syndrome (MetS). The aim of the study was to explore whether a fat challenge containing predominantly saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) or MUFAs plus omega‐3 long‐chain polyunsaturated (LCPUFAs) fatty acids together with a single dose of immediate‐release niacin have a relevant role in postprandial insulin and lipid status in subjects with MetS.RESULTSIn a randomized crossover within‐subject design, 16 men with MetS were given a single dose of immediate‐release niacin (2 g) and ∼15 cal kg−1 body weight meals containing either SFAs, MUFAs, MUFAs plus omega‐3 LCPUFAs or no fat. At baseline and hourly over 6 h, plasma glucose, insulin, C‐peptide, triglycerides, free fatty acids (FFAs), total cholesterol, and both high‐ and low‐density lipoprotein cholesterol were assessed. Co‐administered with niacin, high‐fat meals significantly increased the postprandial concentrations of glucose, insulin, C‐peptide, triglycerides, FFAs and postprandial indices of β‐cell function. However, postprandial indices of insulin sensitivity were significantly decreased. These effects were significantly attenuated with MUFAs or MUFAs plus omega‐3 LCPUFAs when compared with SFAs.CONCLUSIONIn the setting of niacin co‐administration and compared to dietary SFAs, MUFAs limit the postprandial insulin, triglyceride and FFA excursions, and improve postprandial glucose homeostasis in MetS. © 2017 Society of Chemical Industry