bluuebunny/crossref_metadata_2025_split huggingface dataset:
Service Learning in non-formal education in a penitentiary center
Authors: Amores Anabel , De la Calle Maldonado Carmen 
Published: October 2024

Introduction: Prisons should promote educational activities in formal and non-formal settings in accordance with the educational model that is carried out in educational contexts other than prisons. Methodology: The aim of this proposal is to promote social commitment and the development of skills, values and attitudes necessary for social reintegration. To this end, the aim is to develop this activity in the context of non-formal education within the prison environment, through the application of a service-learning methodology. Results and Discussion: Over the course of ten sessions, inmates will be able to work with young people at risk of social exclusion by telling their life stories. In this way, the prisoners will have to work on personal introspection and develop strategies to help prevent delinquency in these young people. Conclusions: All this will favour the development of a social commitment in the persons deprived of liberty and therefore, a series of skills and attitudes that will be necessary for their future reintegration.
Clinical and Pathoanatomical Characteristics of Hypoplastic Left Heart Syndrome a Clinical Case
Authors: Chepelev Alexandr S. , Timofeev Eugene V. , Myaggi Nadezhda A. 
Published: October 2024

Hypoplastic left heart syndrome (HLHS) is a common congenital heart defect, accounting for 4 to 16% of cases, and is one of the most prognostically unfavorable, involving single-­ventricle hemodynamics. HLHS involves hypoplasia of the left ventricle, often in combination with atresia and/or stenosis of the aortic and/or mitral valves, as well as hypoplasia of various parts of the aorta. Intrauterine reduction in blood flow to the left heart structures at early stages of fetal development subsequently leads to severe changes in cardiac structures, often not amenable to surgical correction. Objective. To study the clinical and morphological features of HLHS using a clinical case as an example. Materials and Methods. The patient was a child from the first pregnancy, which was complicated by anemia and first-­trimester preeclampsia. The defect was diagnosed prenatally at 23/24 weeks. At 39 weeks, labor was induced, and the infant was hospitalized in the intensive care unit with symptoms of cardiac and respiratory failure. On the 6th day of life, surgical treatment was performed — a Norwood operation, which is a single-­ventricle hemodynamic surgical correction of the defect. Despite intensive care measures, multiple organ failure progressed, leading to the development of disseminated intravascular coagulation (DIC) and subsequent death on the 10th day of life. Results. Autopsy, including staining of the myocardium with Masson’s trichrome and hematoxylin and eosin, showed pronounced plethora of the microcirculatory vessels with formation of erythrocyte thrombi. Pronounced perivascular edema and fraying of muscle fibers were detected. Fibroelastosis of the endocardium of the left ventricle was observed, characterized by pronounced sclerotic thickening due to significant proliferation of fibrous tissue, with the spread of connective tissue strands into the myocardium. Endocardial fibroelastosis is a component of HLHS and one of the determining factors in the outcome of cardiac surgery. Conclusion. The results of this work indicate the complexity of HLHS as a developmental defect, the severity of its clinical manifestations, and the limited prospects of currently existing surgical treatment methods. These patients may require heart transplantation in the first days of life. A potential alternative to early postnatal heart transplantation may be the development of methods for intrauterine correction of the existing developmental defect.
Germination of chief fire seeds cockscomb cultivar under saline stress conditions induced by sodium chloride
Authors: Barata Gabriel Cruz , Tejo Débora Perdigão , Takane Roberto Jun , Pedro Junior Sérgio , Gomes Gisely Paula , Faria Ricardo Tadeu 
Published: October 2024

Cockscomb plant, Chief fire cultivar, is used as an ornamental, medicinal and non-conventional food plant (NCFP) and is cultivated from the north to the center-west of Brazil; however, these areas suffer from adverse environmental factors such as salinity stress. The objective of this study was to evaluate the seed germination performance and vigor of Celosia cristata L. var. Chief Fire seedlings exposed to salinity during the germination phase. To carry out the work, C. cristata seeds were sown in gerboxes conditioned to increasing doses of sodium chloride (NaCl), obtained by dissolving the salt in distilled water, obtaining electrical conductivities of 2.0; 4.0; 6.0 and 8.0 dS.m-1, constituting the treatments. An additional group was treated only with distilled water and was used as a control for the experiment. The experiment was conducted in a completely randomized design, with a total of five treatments and four replicates of 25 seeds. The parameters assessed were: first germination count, germination, germination speed index (GVI), mean germination time (MGT), length of aerial part and primary root. The data was submitted to analysis of variance and the means were compared using the Scott-Knott test at 5% significance. Based on the results obtained, it can be concluded that the conditions of salt stress induced by NaCl were not harmful to the germination of C. cristata, presenting itself as a species tolerant to water salinity during seed germination, since the variables analyzed showed no signs of deleterious effects as the salt concentration was increased.
Teoretisk kunskap hjälpte mig förstå min praktik
Authors: Henriksson Jenny 
Published: October 2024

I texten berättar jag om hur min tid som doktorand i forskarskolan ReCEC och hur den licentiatuppsats som jag skrev (Henriksson, 2022) har bidragit till att utveckla min förståelse för mig själv som undervisande förskollärare och min undervisningspraktik. I texten lyfts utmaningar och dilemman som förklaras med stöd i licentiatstudiens resultat. Syftet är att visa hur en teoretisk förståelse bidrar till utvecklandet av förskolans undervisningspraktiker.
Right subaxillary small incision on low-age and low-weight infants with congenital heart disease
Authors: Jing Xiaoyong , Li Lun , Wu Yongtao , Wang Zhiyi , Lv Lizhi , Wang Qiang 
Published: October 2024

BackgroundAs a minimally invasive approach to treating congenital heart disease (CHD), the application of the right subaxillary small incision (RSSI) has been developing fast in its indication spectrum. However, RSSI's use is still quite challenging for the surgical treatment of the low-age and low-weight infant patient group.ObjectivesTo investigate the safety and efficacy of performing RSSI surgery, treating CHD for infants with low-age and low-weight.MethodsBetween March 2010 and April 2023, Low-age (≤6 months) and low-weight (≤5 kg) infants with ventricular or atrial septal defect (ASD) as the main diagnosis were retrospectively included in this study. The infants were divided into the RSSI group and the median sternotomy (MS) group. Preoperative conditions, general surgical conditions, perioperative and postoperative outcomes, and long-term follow-up results were compared between the 2 groups.ResultsThe study included 783 cases. Of these, 434 were operated with MS, and 349 were operated with RSSI. A1:1 matched MS group (282 cases) and RSSI group (282 cases) were obtained after performing propensity score matching (PSM). Analysis of the 2 groups after PSM showed the differences in residual ASD/VSD, peak airway pressure, fraction of inspired oxygen/partial pressure of oxygen (PaO2/FiO2), and partial pressure of carbon dioxide (PaCO2) before return to the intensive care unit (ICU) and extubation were not statistically significant between groups, whereas mechanical ventilation time (P &lt; 0.001), ICU stay (P &lt; 0.001), and hospitalization time (P &lt; 0.001) were lower in RSSI group; and the differences in perioperative complication outcomes were not statistically significant. Long-term outcomes revealed the rate of thoracic deformity was higher in the MS group.ConclusionsBased on the appropriate selection of patients, compared with MS, RSSI surgery for the treatment of low-age (≤6 months) and low-weight (≤5 kg) infants with CHD yields favorable therapeutic results without increasing surgical risks and causes less trauma, which deserves to be further promoted and applied.
Inflammatory profile of eosinophils in asthma-COPD overlap and eosinophilic COPD: a multi-omics study
Authors: Sunata Keeya , Miyata Jun , Kawashima Yusuke , Konno Ryo , Ishikawa Masaki , Hasegawa Yoshinori , Onozato Ryuta , Otsu Yo , Matsuyama Emiko , Sasaki Hisashi , Okuzumi Shinichi , Mochimaru Takao , Masaki Katsunori , Kabata Hiroki , Chubachi Shotaro , Arita Makoto , Fukunaga Koichi 
Published: October 2024

IntroductionElevated blood eosinophil levels in patients with chronic obstructive pulmonary disease (COPD) with or without asthma are linked to increased exacerbations and the effectiveness of inhaled corticosteroid treatment. This study aimed to delineate the inflammatory cellular properties of eosinophils in patients with asthma-COPD overlap (ACO) and eosinophilic COPD (eCOPD).MethodsEosinophils were isolated from the peripheral blood of healthy volunteers, patients with non-eCOPD, and those with ACO/eCOPD. Multi-omics analysis involving transcriptomics, proteomics, and lipidomics was performed, followed by bioinformatic data analyses. In vitro experiments using eosinophils from healthy volunteers were conducted to investigate the molecular mechanisms underlying cellular alterations in eosinophils.ResultsProteomics and transcriptomics analyses revealed cellular characteristics in overall COPD patients represented by viral infection (elevated expression of sterol regulatory element-binding protein-1) and inflammatory responses (elevated levels of IL1 receptor-like 1, Fc epsilon receptor Ig, and transmembrane protein 176B). Cholesterol metabolism enzymes were identified as ACO/eCOPD-related factors. Gene Ontology and pathway enrichment analyses demonstrated the key roles of antiviral responses, cholesterol metabolism, and inflammatory molecules-related signaling pathways in ACO/eCOPD. Lipidomics showed the impaired synthesis of cyclooxygenase-derived mediators including prostaglandin E2 (PGE2) in ACO/eCOPD. In vitro assessment confirmed that IL-33 or TNF-α stimulation combined with IL-5 and IFN-γ stimulation induced cellular signatures in eosinophils in ACO/eCOPD. Atorvastatin, dexamethasone, and PGE2 differentially modulated these inflammatory changes.DiscussionACO/eCOPD is associated with viral infection and an inflammatory milieu. Therapeutic strategies using statins and inhaled corticosteroids are recommended to control these pathogenic changes.
Exploring shared biomarkers and shared pathways in insomnia and atherosclerosis using integrated bioinformatics analysis
Authors: Yang Qichong , Liu Juncheng , Zhang Tingting , Zhu Tingting , Yao Siyu , Wang Rongzi , Wang Wenjuan , Dilimulati Haliminai , Ge Junbo , An Songtao 
Published: October 2024

BackgroundInsomnia (ISM) is one of the non-traditional drivers of atherosclerosis (AS) and an important risk factor for AS-related cardiovascular disease. Our study aimed to explore the shared pathways and diagnostic biomarkers of ISM-related AS using integrated bioinformatics analysis.MethodsWe download the datasets from the Gene Expression Omnibus database and the GeneCards database. Weighted gene co-expression network analysis and gene differential expression analysis were applied to screen the AS-related gene set. The shared genes of ISM and AS were obtained by intersecting with ISM-related genes. Subsequently, candidate diagnostic biomarkers were identified by constructing protein–protein interaction networks and machine learning algorithms, and a nomogram was constructed. Moreover, to explore potential mechanisms, a comprehensive analysis of shared genes was carried out, including enrichment analysis, protein interactions, immune cell infiltration, and single-cell sequencing analysis.ResultsWe successfully screened 61 genes shared by ISM and AS, of which 3 genes (IL10RA, CCR1, and SPI1) were identified as diagnostic biomarkers. A nomogram with excellent predictive value was constructed (the area under curve of the model constructed by the biomarkers was 0.931, and the validation set was 0.745). In addition, the shared genes were mainly enriched in immune and inflammatory response regulation pathways. The biomarkers were associated with a variety of immune cells, especially myeloid immune cells.ConclusionWe constructed a diagnostic nomogram based on IL10RA, CCR1, and SPI1 and explored the inflammatory-immune mechanisms, which indicated new insights for early diagnosis and treatment of ISM-related AS.
Feasibility of ctDNA in detecting minimal residual disease and predicting recurrence for colorectal cancer liver metastases
Authors: Kalil Jennifer A. , Krzywon Lucyna , Petrillo Stephanie K. , Tsamchoe Migmar , Zlotnik Oran , Lazaris Anthoula , Metrakos Peter 
Published: October 2024

IntroductionApproximately 50% of patients diagnosed with colorectal cancer develop colorectal cancer liver metastases (CRLM). Although curative intent liver resection provides 5-year survival of 40-50%, up to 70% of patients develop recurrence of CRLM. Detection of minimal residual disease (MRD) is essential for timely, optimized treatment. This study evaluated the feasibility and utility of using circulating tumor DNA (ctDNA) to identify MRD and predict disease recurrence.MethodsPatients with CRLM that underwent liver resection and had known KRAS or PIK3CA mutations were retrospectively identified. Serial blood samples were collected every 3 months following surgery for disease surveillance. ctDNA was isolated from the samples and analyzed with digital PCR (dPCR).ResultsKRAS and PIK3CA mutations were identified by dPCR in 29 patients over 115 timepoints. In patients with detectable ctDNA at time of liver resection, 81% (13/16) developed disease recurrence, while 46% (6/13) of the patients with undetectable ctDNA recurred (p=0.064). Presence of ctDNA was detected in 27.6% (8/29) of the initial postoperative samples. Radiologic recurrence was later diagnosed in 100% (8/8) of these patients, while 52% (11/21) who had undetectable ctDNA postoperatively recurred (p=0.026). Detectable ctDNA postoperatively was associated with a shorter disease-free survival (DFS) of 9 months vs 13 months in patients who had undetectable ctDNA (HR 2.95, 95% CI 1.16-7.49; p=0.02).ConclusionLiquid biopsy using dPCR can identify low levels of ctDNA, enabling early detection of disease recurrence. Additionally, the presence of ctDNA postoperatively was predictive of recurrence. This study corroborates current literature and provides rational for moving toward a clinical trial using ctDNA and dPCR to detect MRD after CRLM resection.
Incidence trends, histological subtypes, and topographical distribution of bladder cancer in Iran: a study based on the Iranian National Cancer Registry during 2006-2015
Authors: Naserghandi Alvand , Azizmohammad Looha Mehdi , Jameie Melika , Moradian Haft Cheshmeh Zeynab , Namakin Kosar , Golmakani Najmeh , Feyzi Aydin , Shabanipour Hadis , Tofighi Zavareh Mohammad Amin , Allameh Farzad , Akbari Mohammad Esmaeil 
Published: October 2024

BackgroundBladder cancer (BCa) is a significant public health concern. This study aimed to analyze the incidence trends, histological subtypes, and topographical distribution of BCa in Iran over a decade.MethodsThis retrospective registry-based study analyzed data on BCa patients diagnosed between March 20, 2006, and March 20, 2015. Following data quality control, age-standardized incidence rates (ASIRs) were calculated for BCa overall, by sex and histological subtype using the new World Health Organization (WHO) standard population.ResultsWe identified 51,379 BCa cases (81.97% male) with a mean age of 65.10 ± 14.89 years. The overall ASIR was 8.92 per 100,000 (95% CI: 8.84-9.00). While a modest increase in ASIR was observed overall (8.77 in 2006 to 9.64 in 2015) and among males (14.13 in 2006 to 15.95 in 2015) during the study period, males consistently had a significantly higher ASIR compared to females (approximately 4.5:1 ratio). BCa incidence showed a progressive increase across older age groups, particularly those aged 40-44 to 80-84 years. Urothelial cell carcinoma (UCC) was the most prevalent histological type (ASIR: 8.19 to 7.93), followed by adenocarcinoma (AC; ASIR: 0.13 to 0.11). Squamous cell carcinoma (SCC) displayed a decreasing trend (ASIR: 0.11 to 0.06). Both UCC and AC were more frequent in males (approximately 5 and 3 times higher than females, respectively). Malignant neoplasm of the bladder, unspecified, constituted over 95% of BCa topography classifications.ConclusionThis study identified a modest rise in BCa incidence, with male predominance and a higher burden in older adults. Further investigation into potential risk factors contributing to this increase is warranted.
The mutated in colorectal cancer (MCC) gene can serve as a potential biomarker of glioblastoma
Authors: Nguyen Huonggiang , Huang Qingzhi , Juang Uijin , Gwon Suhwan , Jung Woohyeong , Lee Soohyeon , Lee Beomwoo , Kwon So Hee , Kim In Soo , Park Jongsun , Kim Seon-Hwan 
Published: October 2024

IntroductionThe mutated in colorectal cancer (MCC) gene was initially identified as a candidate tumor suppressor gene in colorectal cancer, acting as a negative regulator of cell cycle progression. However, its functional roles in brain tumors, particularly glioblastoma, remain largely unexplored. This study reveals a significant association between MCC status and glioblastoma.MethodsWe explored MCC expression in the glioblastoma database, patient samples, and cell lines. We investigated the proliferation and migration of the cell lines in MCC gene knockdown using small interfering RNA.ResultsIn vitro analyses revealed elevated protein and mRNA levels of MCC in several glioblastoma cell lines (U118MG and T98G). Silencing MCC expression via siRNA-mediated knockdown resulted in increased proliferation and migration of these cell lines. Supporting these findings, analyses of The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), and Genotype-Tissue Expression (GTEx) databases confirmed higher MCC expression in glioblastoma tumors than in normal brain tissue. Importantly, we observed that high MCC expression was associated with poor prognosis in glioblastoma patients, highlighting its potential role in disease progression. Additionally, this study identifies a nuclear localization of MCC in the glioblastoma cell line.DiscussionThese findings indicate that MCC expression is significantly upregulated in glioblastoma and may play a role in its pathophysiology, warranting further investigation.
Causal links between circulatory inflammatory cytokines and risk of digestive polyps: a Mendelian randomization analysis
Authors: Yan Ziqi , Zheng Hongming , Feng Jieni , Li Yiting , Hu Zhifan , Wu Yuan , Liao Guibin , Miao Taosheng , Qiu Zexin , Mo Qiaolan , Li Jia , Lai Ailin , Lu Yue , Chen Bin 
Published: October 2024

BackgroundThere is a high morbidity of polyps in the digestive tract, and certain subtypes of polyps are thought to induce cancer progression and often recur, which may be associated with chronic inflammation. Mendelian randomization (MR) can help identify potential causative relationships and inform early treatment action.MethodsWe performed a bidirectional two-sample MR analysis implementing the results from genome-wide association studies for 41 serum cytokines from 8,293 Finnish individuals, and three types of polyps from European ancestry, respectively, including gastric polyp (6,155 cases vs. 341,871 controls), colonic polyp (22,049 cases vs. 332,368 controls) and gallbladder polyp (458 cases vs. 340,083 controls). Inverse-variance weighted (IVW), weight median (WM), and MR-Egger methods were used for calculating causal estimates. Furthermore, Bayesian model averaging MR (MR-BMA) method was employed to detect the dominant causal circulatory cytokines with adjustment for pleiotropy effects.ResultsOur univariable MR using inverse-variance weight method identified causal associations of IL-2ra (OR: 0.892, 95%CI: 0.828–0.961, p = 0.003), MIG (OR: 1.124, 95%CI: 1.046–1.207, p = 0.001) and IL-18 (OR: 0.912, 95%CI: 0.852–0.977, p = 0.008) with gastric polyp, MIP1b (OR: 0.956, 95%CI: 0.927–0.987, p = 0.005) and IL-6 (OR: 0.931, 95%CI: 0.870–0.995, p = 0.035) with colonic polyp and IL-9 (OR: 0.523, 95%CI: 0.345–0.794, p = 0.0007) with gallbladder polyp. Finally, our MR-BMA analysis prioritized MIG (MIP = 0.332, MACE = 0.022; PP: 0.264, MSCE = 0.059), IL-18 (MIP = 0.302, MACE = −0.020; PP: 0.243, MSCE = −0.059) and IL-2ra (MIP: 0.129; MACE: −0.005; PP: 0.112, MSCE: −0.031) for gastric polyp, and MIP1b (MIP = 0.752, MACE = −0.033; PP: 0.665, MSCE = −0.044) and IL-6 (MIP: 0.196; MACE: −0.012; PP: 0.140, MSCE: −0.064) for colonic polyp, and IL-9 (MIP = 0.936, MACE = −0.446; PP: 0.781, MSCE = −0.478) for gallbladder polyp as the top-ranked protective factors.ConclusionOur research advances the current understanding of the function of certain inflammatory biomarker pathways in the genesis and malignant mutation of polyps in the digestive tract. Deeper substantiation is necessary to assess the potential of these cytokines as pharmacological or lifestyle targets for digestive polyps prevention.
Gender differences in autonomic and psychological stress responses among educators: a heart rate variability and psychological assessment study
Authors: Calderón-García Andrea , Álvarez-Gallardo Estela , Belinchón-deMiguel Pedro , Clemente-Suárez Vicente Javier 
Published: October 2024

IntroductionThis study explores the gender differences in psychological stress perception and autonomic modulation among teachers.MethodsUtilizing heart rate variability (HRV) as a measure of autonomic function and a suite of validated psychological tests, the study examines the discrepancies in stress, anxiety, burnout, and personality traits between male and female educators.ResultsResults indicate that despite higher reported levels of stress and anxiety, women demonstrate a higher HRV, suggesting a stronger parasympathetic response.DiscussionThese findings highlight the complex interplay between psychological stressors and physiological responses, emphasizing the need for gender-specific interventions in stress management within the educational sector. Implications for enhancing educators’ well-being and performance through tailored strategies are discussed.