bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of Oxygen Vacancy‐Enriched POM Heterostructure with Enhanced Metal–Support Interactions via Supercritical Anti‐Solvent Method for Ultratrace Cysteine Analysis
Authors: Xiong Wei‐Guang , Li Chang‐Yong , Chen Ai‐Zheng , Wang Shi‐Bin 
Published: September 2024

AbstractThis study introduces a novel polyoxometalate (POM) heterostructure designed to address challenges in ultratrace analysis and sensor stability. Supercritical anti‐solvent and hydrothermal methods are employed to fabricate a composite that enhances stability, sensitivity, and selectivity for cysteine (Cys) detection. The optimization of inter‐component interactions and improved dispersibility contributes to superior stability and longevity. These preparation techniques increase the total oxygen vacancy density, which facilitates the migration of surface oxygen vacancies and promotes the electrocatalytic process. Additionally, tuning the band structure effectively suppresses electron–hole recombination, thereby enhancing catalytic capability. The integration of potassium phosphotungstate (KPW) as an electron transport mediator results in a stable “point‐surface” loading structure, increasing active sites and improving the material's specific surface area and catalytic efficiency. The Cu/Zr nanoparticle‐grafted KPW composite (CZPW) demonstrates excellent performance, achieving an ultratrace detection limit of 30.6 pM and a broad linear detection range from 50 pM to 1 mM. Overall, this study elucidates the mechanisms of supercritical preparation and its impact on electrocatalytic Cys reactions, providing valuable insights into the development of highly effective and stable biosensors.
In Vivo Monitoring and Magnetically‐Enhanced Delivery of CAR T‐Cells to Solid Tumor
Authors: Chen Huan , Machado Alice , An Dongjie , Becharef Sonia , Autret Gwennhael , Ayollo Dmitry , Razafindrakoto Sarah , Nizard Philippe , Carn Florent , Luo Yun , Pendino Frédéric , Mangeney Claire , Kolosnjaj‐Tabi Jelena , Donnadieu Emmanuel , Gazeau Florence 
Published: September 2024

AbstractChimeric antigen receptor (CAR) T‐cell therapy demonstrates efficacy for the treatment of hematologic cancers, but remains challenging in solid tumors because of the hostile tumor microenvironment and heterogeneity that restrict the infiltration and activity of CAR T‐cells. The lack of clinical imaging methods to monitor CAR T‐cell therapy in patients limits the potential to enhance the therapeutic approach and predict patient response. Here, a straightforward, non‐invasive method for Magnetic Resonance Imaging (MRI) tracking and magnetic targeting of CAR T‐cells to enhance T‐cell trafficking to solid tumors, in a setting that can be deployed in clinics, is reported. Iron oxide nanoparticles are loaded into T‐cells, allowing MRI detection and magnetic cell guidance, while preserving T‐cell viability, functions, and CAR specificity to target epidermal growth factor receptor (EGFR)‐expressing tumors. Longitudinal MRI monitoring of T‐cells over 14 days reveals differences in tumor infiltration rates and antitumor efficacy between EGFR‐targeted CAR T‐cells and non‐transduced T‐cell. Moreover, an external magnet placed over the tumor enhances the infiltration of CAR T‐cells and increases their antitumor effect. MRI monitoring and magnetic guidance represent a dual‐pronged clinically feasible therapeutic strategy to control and promote CAR T‐cells delivery into solid tumors, thereby improving the precision, efficacy, and safety of the treatment.
One‐Pot Transformation Strategy of o‐Hydroxyphenyl Propargylamines for Modular Access to 3‐Functionalized Benzofurans
Authors: Wu Mengdi , Liu Yanan , Kong Yangzilin , Fei Binbin , Tang Qiang , He Xinwei 
Published: September 2024

AbstractA one‐pot strategy to C3‐functioanlized benzofurans is achieved from o‐hydroxyphenyl propargylamines (o‐HPPAs). 3‐Aminobenzofurans were obtained in 64–96% yields through the cascade alkyne‐allene tautomerization and 5‐exo‐dig annulation of o‐HPPAs in the presence of KOH/NaOtBu as the fruitful base combination. Moreover, with benzenesulfinates and diphenylphosphine oxide as nucleophiles, a one‐pot protocol towards these derivatives was also demonstrated through a cascade 1,4‐conjugate addition/5‐exo‐dig annulation/aromatization process. Additionally, a gram‐scale synthesis and further transformation into other valuable functionalized benzofurans were conducted to show the potential in organic synthesis of these developed synthetic approaches.
Macrophage‐Hepatocyte Circuits Mediated by Grancalcin Aggravate the Progression of Metabolic Dysfunction Associated Steatohepatitis
Authors: Su Tian , He Yue , Wang Min , Zhou Haiyan , Huang Yan , Ye Mingsheng , Guo Qi , Xiao Ye , Cai Guangping , Zhao Mingyang , Wang Jianping , Luo Xianghang 
Published: September 2024

AbstractThe dynamic interplay between parenchymal hepatocytes and non‐parenchymal cells (NPCs), such as macrophages, is an important mechanism for liver metabolic homeostasis. Although numerous endeavors have been made to identify the mediators of metabolic dysfunction associated steatohepatitis (MASH), the molecular underpinnings of MASH progression remain poorly understood, and therapies to arrest MASH progression remain elusive. Herein, it is revealed that the expression of grancalcin (GCA) is upregulated in the macrophages of patients and rodents with MASH and correlates with MASH progression. Notably, the administration of recombinant GCA aggravates the development of MASH, whereas, Gca deletion in myeloid cells blunts liver steatosis and inflammation in multiple MASH murine models. Mechanistically, GCA activates macrophages via TLR9‐NF‐κB signaling, and the activated macrophages promote hepatocyte lipid accumulation and apoptosis via secretion of Interleukin‐6(IL‐6), Tumor Necrosis Factor α (TNFα), and Interleukin‐1β(IL‐1β), thereby leading to hepatic steatosis and inflammation. Finally, the therapeutic administration of antibody blocking GCA effectively halts the progression of MASH. Collectively, these findings implicate GCA as a crucial mediator of MASH and clarify a new metabolic signaling axis between the hepatocytes and macrophages, implying that GCA can emerge as a particularly interesting putative therapeutic target for reversing MASH progression.
Morphometry of medial temporal lobe subregions using high‐resolution T2‐weighted MRI in ADNI3: Why, how, and what's next?
Authors: Yushkevich Paul A. , Ittyerah Ranjit , Li Yue , Denning Amanda E. , Sadeghpour Niyousha , Lim Sydney , McGrew Emily , Xie Long , DeFlores Robin , Brown Christopher A. , Wisse Laura E. M. , Wolk David A. , Das Sandhitsu R. ,   for the Alzheimer's Disease Neuroimaging Initiative
Published: September 2024

AbstractThis paper for the 20th anniversary of the Alzheimer's Disease Neuroimaging Initiative (ADNI) provides an overview of magnetic resonance imaging (MRI) of medial temporal lobe (MTL) subregions in ADNI using a dedicated high‐resolution T2‐weighted sequence. A review of the work that supported the inclusion of this imaging modality into ADNI Phase 3 is followed by a brief description of the ADNI MTL imaging and analysis protocols and a summary of studies that have used these data. This review is supplemented by a new study that uses novel surface‐based tools to characterize MTL neurodegeneration across biomarker‐defined AD stages. This analysis reveals a pattern of spreading cortical thinning associated with increasing levels of tau pathology in the presence of elevated amyloid beta, with apparent epicenters in the transentorhinal region and inferior hippocampal subfields. The paper concludes with an outlook for high‐resolution imaging of the MTL in ADNI Phase 4.Highlights
As of Phase 3, the Alzheimer's Disease Neuroimaging Initiative (ADNI) magnetic resonance imaging (MRI) protocol includes a high‐resolution T2‐weighted MRI scan optimized for imaging hippocampal subfields and medial temporal lobe (MTL) subregions.
These scans are processed by the ADNI core to obtain automatic segmentations of MTL subregions and to derive morphologic measurements.
More detailed granular examination of MTL neurodegeneration in response to disease progression is achieved by applying surface‐based modeling techniques.
Surface‐based analysis of gray matter loss in MTL subregions reveals increasing and spatially expanding patterns of neurodegeneration with advancing stages of Alzheimer's disease (AD), as defined based on amyloid and tau positron emission tomography biomarkers in accordance with recently proposed criteria.
These patterns closely align with post mortem literature on spread of pathological tau in AD, supporting the role of tau pathology in the presence of elevated levels of amyloid beta as the driver of neurodegeneration.
Interdependence of Support Wettability ‐ Electrodeposition Rate‐ Sodium Metal Anode and SEI Microstructure
Authors: Lo Chang‐An , Wang Yixian , Kankanallu Varun R. , Singla Aditya , Yen Dean , Zheng Xiaoyin , Naik Kaustubh G. , Vishnugopi Bairav S. , Campbell Callum , Raj Vikalp , Zhao Chonghang , Ma Lu , Bai Jianming , Yang Feipeng , Li Ruipeng , Ge Mingyuan , Watt John , Mukherjee Partha P. , Mitlin David , Karen Chen‐Wiegart Yu‐chen 
Published: September 2024

AbstractThis study examines how current collector support chemistry (sodiophilic intermetallic Na2Te vs. sodiophobic baseline Cu) and electrodeposition rate affect microstructure of sodium metal and its solid electrolyte interphase (SEI). Capacity and current (6 mAh cm−2, 0.5–3 mA cm−2) representative of commercially relevant mass loading in anode‐free sodium metal battery (AF‐SMBs) are analyzed. Synchrotron X‐ray nanotomography and grazing‐incidence wide‐angle X‐ray scattering (GIWAXS) are combined with cryogenic ion beam (cryo‐FIB) microscopy. Highlighted are major differences in film morphology, internal porosity, and crystallographic preferred orientation e.g. (110) vs. (100) and (211) with support and deposition rate. Within the SEI, sodium fluoride (NaF) is more prevalent with Te−Cu versus sodium hydride (NaH) and sodium hydroxide (NaOH) with baseline Cu. Due to competitive grain growth the preferred orientation of sodium crystallites depends on film thickness. Mesoscale modeling delineates the role of SEI (ionic conductivity, morphology) on electrodeposit growth and onset of electrochemical instability.
Propane Dehydrogenation on PtxZny Active Sites in Silicalite‐1
Authors: Liu Yilang , Bhowmick Antara , Liu Dongxia , Caratzoulas Stavros , Vlachos Dionisios G. 
Published: September 2024

AbstractThe improvement of Pt‐based catalysts for propane dehydrogenation (PDH) has progressed by recent investigations that have identified Zn as a promising promoter for Pt subnanometer catalysts. It is desirable to gain insights into the structure, stability, and activity of such active sites and the factors that influence them, such as Zn : Pt ratio, Pt coordination and nuclearity. Here, we employ density functional theory and microkinetic simulations to investigate the stability of PtxZny (x=1–3, y=0–3) active sites grafted on silanols of Silicalite‐1 and the PDH activity of Pt. We find that the coordination of a Pt atom to a nest of grafted Zn(II) atoms increases the stability of the Pt1Zny sites, whose activity is similar for y=0–2 and drops dramatically for y>2. We further demonstrate, via linear scaling relations and microkinetic simulations, that the turnover frequency obeys a volcano law as a function of propylene binding strength. The Pt2Zn1 and Pt3Zn1 sites are stable and exhibit activity similar to Pt1Zn2, but only Pt1Zn2 manifests reaction kinetics consistent with experimental data, strongly suggesting the active site composition in the synthesized catalyst samples. The methodology presented here suggests a general strategy for deducing active site information such as composition through simple kinetic experiments.
Synergistic Tuning of Microstructure and Morphology in Carbon Molecular Sieve Hollow Fibers for Propylene/Propane Separation
Authors: Liu Zhongyun , Cao Yuhe , Koros William J. 
Published: September 2024

AbstractAsymmetric carbon molecular sieve (CMS) hollow fiber membranes with tunable micro‐ and macro‐structural morphologies for energy efficient propylene‐propane separation are reported here. A sub‐glass transition temperature (sub‐Tg) thermal oxidative crosslinking strategy enables simultaneous optimization of the intrinsic molecular sieving properties while also reducing the thickness of the CMS “skin” derived from the 6FDA : BPDA/DAM polyimide precursors. Such synergistic tuning of CMS microstructure and macroscopic morphology of CMS hollow fibers enables significantly increased propylene permeance (reaching 186.5 GPU) while maintaining an appealing propylene/propane selectivity of 13.3 for 50/50 propylene/propane mixed gas feeds. Our findings reveal a more refined and versatile tool than available with previous O2‐doping pretreatments. The advanced approach here should be broadly useful to other polyimide precursors and diverse gas pairs.
Molecular Imaging of Renin Activity using Fluorogenic Nanoprobes for Precision Antihypertensive Therapy
Authors: Zhou Ya , Xu Weiping , Ruan Bankang , Zhu Lijuan , Jiang Yuyan , Cai Hui , Huang Jiaguo 
Published: September 2024

AbstractLife‐threatening hypertension remains inadequately controlled in clinics due to its heterogeneous renin levels. Rapid stratification of hypertension through renin analysis is crucial for effective personalized treatment, yet an ultrasensitive detection approach is currently lacking. Here, we report activatable renin nanoprobes (ARNs) for non‐invasive and ultrasensitive profiling of renin activity and guiding antihypertensive treatment decision through near‐infrared fluorescence (NIRF) in vivo imaging and in vitro urinalysis. ARNs are intrinsically non‐fluorescent due to NIRF reporter connected to a gold nanocluster through a renin‐responsive peptide. In hyperreninemia mouse models, ARNs specifically react with renin to liberate the renal clearable NIRF reporter for accurate renin detection that outperforms the gold standard radioimmunoassay. Such specific and sensitive detection also enables imaging‐based high‐throughput screening of antihypertensive drugs. In hypertensive rat models, ARNs enable ultrasensitive detection of both plasma and urinary renin, facilitating renin‐guided precision treatment and significantly improving hypertension control rate (90 % versus 58 %). Our nanoprobe platform holds great potential for assisting clinicians in rapidly and accurately classifying hypertensive patients and improving outcomes through tailored treatment selection.
Hypoxia Promotes the Expression of ADAM9 by Tubular Epithelial Cells, Which Enhances Transforming Growth Factor β1 Activation and Promotes Tissue Fibrosis in Patients With Lupus Nephritis
Authors: Umeda Masataka , Karino Kohei , Satyam Abhigyan , Yoshida Nobuya , Hisada Ryo , Bhargava Rhea , Vichos Theodoros , Kunzler Ana Laura , Igawa Takashi , Ichinose Kunihiro , Torigoe Kenta , Nishino Tomoya , Maeda Takahiro , Owen Caroline A. , Abdi Reza , Kawakami Atsushi , Tsokos George C. 
Published: September 2024

ObjectiveEnhanced expression of transforming growth factor (TGF) β in the kidneys of patients with lupus nephritis (LN) can lead to progressive fibrosis, resulting in end‐organ damage. ADAM9 activates TGFβ1 by cleaving the latency‐associated peptide (LAP). We hypothesized that ADAM9 in the kidney may accelerate fibrogenesis by activating TGFβ1.MethodsWe assessed the expression of ADAM9 in the kidneys of mice and humans who were lupus prone. In vitro experiments were conducted using tubular epithelial cells (TECs) isolated from mice and explored the mechanisms responsible for the up‐regulation of ADAM9 and the subsequent activation of TGFβ1. To assess the role of ADAM9 in the development of tubular‐intestinal fibrosis in individuals with LN, we generated MRL/lpr mice who were Adam9 deficient.ResultsADAM9 was highly expressed in tubules from MRL/lpr mice. The transcription factor hypoxia‐inducible factor–1α was found to promote the transcription of ADAM9 in TECs. TECs from mice who were Adam9 deficient and exposed to the hypoxia mimetic agent dimethyloxalylglycine failed to cleave the LAP to produce bioactive TGFβ1 from latent TGFβ1. Coculture of TECs from mice who were Adam9 deficient with fibroblasts in the presence of dimethyloxalylglycine and latent TGFβ1 produced decreased amounts of type I collagen and α–smooth muscle actin (SMA) by fibroblasts. MRL/lpr mice who were Adam9 deficient showed reduced interstitial fibrosis. At the translational level, ADAM9 expression in tissues and urine of patients with LN was found to increase.ConclusionHypoxia promotes the expression of ADAM9 by TECs, which is responsible for the development of interstitial fibrosis in patients with LN by enhancing the TGFβ1 activation, which promotes fibroblasts to produce collagen and α‐SMA.image
Methods for Analyzing PFAS in Water
Authors: Lipps William , Adams Hunter , Thomas Dave , Urban Sinisa 
Published: September 2024

Key TakeawaysAs the hazards of per‐ and polyfluoroalkyl substances—widely known as PFAS—are better understood, regulations and guidelines for addressing them will continue to evolve.Water utilities play a significant role in PFAS mitigation by incorporating detection, monitoring, and treatment techniques into their operations.Many methods for PFAS analysis are in place. A detailed explanation and comparison of each can help utilities determine the best approaches for their particular circumstances.
Uropathogen antibiogram regional variations—Are Australian antimicrobial guidelines appropriate?
Authors: Wei Gavin , Sewell James Antony Sidney , Bartolo Caroline , Pearce Amelia , Harris Owen , Grills Richard 
Published: September 2024

AbstractObjectivesThe objectives of this study are as follows: to assess the uropathogen antibiogram at two tertiary hospitals in Victoria to look at the difference in susceptibility patterns, to assess whether national guideline recommendations were applicable and to assess the feasibility of local antibiogram analysis to guide development of hospital‐specific guidelines for empirical treatment of urosepsis and for pre‐operative prophylaxis for urological procedures.Patients and methodsAll positive urine cultures analysed at Barwon Health and Monash Health, two tertiary hospitals in regional and metropolitan Victoria, Australia, respectively, between January 2019 and December 2020 were retrospectively identified. Data obtained included the organism cultured and their susceptibility profiles.ResultsThree thousand seven hundred and seventy‐seven positive urine cultures from Barwon Health and 6821 from Monash Health were identified. The most common uropathogen was Escherichia coli, which was cultured in 53.4% and 59.1% of urine cultures at Barwon Health and Monash Health, respectively. The main differences observed were in Enterococcus spp., which were cultured in 8.8% and 4.9% of cultures at Barwon Health and Monash Health, respectively, and Candida spp. in 4.2% and 1.5% of cultures at Barwon Health and Monash Health, respectively. The largest differences were found in fluoroquinolone resistance with 12.1% of organisms resistant to ciprofloxacin at Barwon Health compared to 6.4% at Monash Health and 7.1% of organisms resistant to vancomycin compared to 20.1% at Barwon Health and Monash Health, respectively.ConclusionThis study demonstrates that there is considerable variability in the uropathogens and their antimicrobial susceptibility profile in two large health services in the same state. We recommend that each centre performs regular analysis of their uropathogen antibiogram to develop local guidelines for treatment and pre‐operative prophylaxis for uropathogens.
The modulation of lung cancer cell motility by Calponin 3 is achieved through its ability to sense and respond to changes in substrate stiffness
Authors: Chi Yinxiu , Wang Xianhui , Shao Xiaoyun , Zhang Dongliang , Han Jingjing , Deng Linhong , Yang Liucai , Qu Xuebin 
Published: September 2024

AbstractThe influence of extracellular matrix (ECM) stiffness on cell behavior is a well‐established phenomenon. Tumor development is associated with the stiffening of the ECM. However, the understanding of the role of biomechanical behavior and mechanotransduction pathways in the oncogenesis of tumor cells remains limited. In this study, we constructed in vitro models using Polydimethylsiloxane substrates to create soft and stiff substrates. We then evaluated the migration of lung cancer cells A549 using video‐microscopy and transwell assays. The mechanical properties were assessed through the utilization of atomic force microscopy, Optical Magnetic Twisting Cytometry, and traction force analysis. Additionally, the expression of Calponin 3 (CNN3) was evaluated using reverse transcription‑quantitative PCR and immunofluorescence techniques. Our observations indicate that the presence of a stiff substrate enhances A549 motility, as evidenced by increased stiffness and traction force in A549 cells on the stiff substrate. Furthermore, we observed a decrease in CNN3 expression in A549 cells on the stiff substrate. Notably, when CNN3 was overexpressed, it effectively inhibited the migration and invasion of A549 cells on the stiff substrate. The results of our study provide novel perspectives on the mechanisms underlying cancer cell migration in response to substrate mechanical properties.
Neurosarcoidosis: Clinical, biological, and MRI presentation of central nervous system disease in a national multicenter cohort
Authors: Dos santos Amélie , Courtin Edouard , Ruet Aurélie , Duffau Pierre , Mathey Guillaume , Bekkour Ines , Ciron Jonathan , Michel Laure , Blanc François Xavier , Aguilar Jésus , Lejeune Pascal , Marc Guillaume , Laplaud David , Magot Armelle , Hamidou Mohamed , Wiertlewski Sandrine 
Published: September 2024

AbstractIntroductionNeurosarcoidosis (NS) is a systemic inflammatory granulomatous disease affecting of patients with sarcoidosis. Its diagnosis is difficult as there is no specific test for it. Because of its rarity, the management of NS has so far only been described in case series and short retrospective cohorts. The objective of this study is description of the clinical, paraclinical presentation and the therapeutic management of central nervous system (CNS) involvement in NS patients in France.MethodsThis multicenter, retrospective, observational study involved patients hospitalized between 2010 and 2019 with a diagnosis of sarcoidosis and CNS involvement.ResultsWe included 118 patients (38 with isolated NS, 80 with NS associated with systemic sarcoidosis). NS was the initial presentation in 78% of patients, with cranial nerve involvement (36%), medullary symptoms (23%), and seizures (21%). Twenty‐one percent of the patients had already been diagnosed with systemic sarcoidosis. The most frequent biological abnormality was lymphopenia (62.5%), while angiotensin‐converting enzyme was increased in 21%. Meningitis was present in 45% and hyperproteinorachia in 69.5% of cases. MRI mainly revealed white matter abnormalities and leptomeningeal enhancement (34%). Corticosteroids were the most useful treatment, and immunosuppressive agents were used in steroid‐resistant patients and to limit side effects. Methotrexate, cyclophosphamide, and anti‐TNFα were also used, exhibiting good efficacy.ConclusionsThis cohort contributes to a better understanding of the clinical phenotype and associated imaging and biological abnormalities. Sharing of clinical, biological, and imaging data, as well as the therapeutic responses, of patients with NS helps to better understand and manage this disease that affects a small number of patients per center. A database project could be implemented in the future to enable this.