bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR‐19a Affects Hepatocyte Autophagy via Regulating lncRNA NBR2 and AMPK/PPARα in D‐GalN/Lipopolysaccharide‐Stimulated Hepatocytes
Authors: Liu Yan‐Min , Ma Jin‐Hui , Zeng Qing‐Lei , Lv Jun , Xie Xu‐Hua , Pan Ya‐Jie , Yu Zu‐Jiang 
Published: June 2017

ABSTRACTThis study aims to evaluate the potential involvement and regulatory mechanism of miR‐19a in hepatocytes autophagy of acute liver failure (ALF). The in vitro hepatocytes injury model of primary hepatocyte and hepatocytes line HL‐7702 was established by D‐galactosamine (D‐GalN) and lipopolysaccharide (LPS) co‐treatment. Relative expression level of miR‐19a and NBR2 was determined by qRT‐PCR. Protein expression of AMPK/PPARα and autophagy‐related gene was determined by Western blot. In hepatic tissue of 20 ALF patients and D‐GalN/LPS‐stimulated hepatocytes, miR‐19a was upregulated and NBR2 was downregulated. D‐GalN/LPS stimulation caused the inactivation of AMPK/PPARα signaling and the decrease of autophagy‐related LC3‐II/LC3‐I ratio and beclin‐1 expression in hepatocytes. The expression of both AMPK/PPARα and NBR2 were negatively controlled by miR‐19a overexpression or knockdown. Moreover, both NBR2 and PPARα were targeted regulated by miR‐19a according to luciferase reporter assay. In D‐GalN/LPS‐stimulated hepatocytes, AMPK activation promoted PPARα expression. AMPK inactivation inhibited the pro‐autophagy effect of miR‐19a and caused the decrease of LC3‐II/LC3‐I ratio and beclin‐1 expression. PPARα activation abrogated the anti‐autophagy effect of miR‐19a mimic and caused the increase of LC3‐II/LC3‐I ratio and beclin‐1 expression. NBR2 knockdown reversed the anti‐autophagy impact of miR‐19a inhibitor and caused the decrease of LC3‐II/LC3‐I ratio and beclin‐1 expression. In summary, our data suggested that miR‐19a negatively controlled the autophagy of hepatocytes attenuated in D‐GalN/LPS‐stimulated hepatocytes via regulating NBR2 and AMPK/PPARα signaling. J. Cell. Biochem. 119: 358–365, 2018. © 2017 Wiley Periodicals, Inc.
Lycopene Protects Keratinocytes Against UVB Radiation‐Induced Carcinogenesis via Negative Regulation of FOXO3a Through the mTORC2/AKT Signaling Pathway
Authors: Chen Ping , Xu Shina , Qu Jinlong 
Published: June 2017

ABSTRACTLycopene, one of the most potent anti‐oxidants, has been reported to exhibit potent anti‐proliferative properties in a wide range of cancer cells through modulation of the cell cycle and apoptosis. Forkhead box O3 (FOXO3a) plays a pivotal role in modulating the expression of genes involved in cell death. Herein, we investigated the role of FOXO3a signaling in the anti‐cancer effects of lycopene. Results showed that lycopene pretreatment attenuated UVB‐induced cell hyper‐proliferation and promoted apoptosis, accompanied by decreased cyclin‐dependent kinase 2 (CDK2) and CDK4 complex in both human keratinocytes and SKH‐1 hairless mice. FOXO3a is phosphorylated in response to UVB irradiation and sequestered in the cytoplasm, while lycopene pretreatment rescued this sensitization. Gene ablation of FOXO3a attenuated lycopene‐induced decrease in cell hyper‐proliferation, CDK2, and CDK4 complex, indicating a critical role of FOXO3a in the lycopene‐induced anti‐proliferative effect of keratinocytes during UVB irradiation. Transfection with FOXO3a siRNA inhibited the lycopene‐induced increase in cell apoptosis, BAX and cleaved PARP expression. Moreover, loss of AKT induced further accelerated lycopene‐induced FOXO3a dephosphorylation, while loss of mechanistic target of rapamycin complex 2 (mTORC2) by transfection with RICTOR siRNA induced levels of AKT phosphorylation comparable to those obtained with lycopene. In contrast, overexpression of AKT or mTORC2 decreased the effects of lycopene on the expression of FOXO3a as well as AKT phosphorylation, suggesting that lycopene depends on the negative modulation of mTORC2/AKT signaling. Taken together, our findings demonstrate that the mTORC2/AKT/FOXO3a axis plays a critical role in the anti‐proliferative and pro‐apoptotic effects of lycopene in UVB‐induced photocarcinogenesis. J. Cell. Biochem. 119: 366–377, 2018. © 2017 Wiley Periodicals, Inc.
Coping and Psychopathological Profile in Nonsuicidal Self‐Injurious Chilean Adolescents
Authors: Castro Karla , Kirchner Teresa 
Published: June 2017

ObjectiveThe aims of this study were to establish the roles of coping typologies and the psychological problems associated with nonsuicidal self‐injury (NSSI) and to identify adolescents who are at greater risk of engaging in this practice.MethodThe total sample comprised 965 adolescents (57% girls, aged 12–18 years) from several Chilean schools who answered the Inventory of Statements About Self‐Injury questionnaire.ResultsYoung people with and without NSSI present different coping patterns, especially when compared by gender. Adolescents with repetitive NSSI (R‐NSSI) report using escape, search for friendship, and venting feelings as coping strategies as well as a more avoidant style and less search for family support than those adolescents without NSSI. Adolescents who present avoidance coping typology are three times more likely (relative risk = 3.5) to engage in NSSI than adolescents who present approach coping typology. R‐NSSI adolescents present higher scores on psychological symptomatology (within clinical ranges) than their N‐NSSI counterparts. Girls were more likely to present psychological symptoms than boys.ConclusionThe authors highlight the usefulness of coping typologies for the detection of high‐risk populations for NSSI as well as the need to consider the gender variable. Professionals who detect clinical levels of symptomatology should also assess the presence of NSSI.
Photocatalytic degradation of saccharin under UV‐LED and blacklight irradiation
Authors: Davididou Konstantina , McRitchie Calum , Antonopoulou Maria , Konstantinou Ioannis , Chatzisymeon Efthalia 
Published: June 2017

AbstractBACKGROUNDThe photocatalytic treatment of the artificial sweetener saccharin (SAC), an emerging environmental contaminant, was investigated. UVA irradiation was provided by an environmentally friendly light‐emitting diode (UV‐LED), whose efficiency was compared with a conventional blacklight fluorescent lamp (UV‐BL).RESULTSThe effect of the initial SAC concentration (2.5–10 mg L‐1), TiO2 concentration (0–500 mg L‐1), water matrix (absence/presence of humic acids), and treatment time on process efficiency was evaluated. Under the best conditions assayed ([SAC]0 = 2.5 mg L‐1, [TiO2] = 250 mg L‐1), SAC was degraded within 20 and 90 min under UV‐LED and UV‐BL irradiation, respectively. Liquid chromatography‐high resolution mass spectrometry (LC‐HR/MS) revealed that SAC degradation proceeds via hydroxylation of the phenyl ring, cleavage of C–N bond and further oxidation reactions. Finally, UV‐LED was found to be up to 16 times more energy efficient than UV‐BL.CONCLUSIONSIn all cases, UV‐LED achieved higher photocatalytic efficiency, in terms of organic degradation, and was found to be significantly more energy and cost efficient than conventional UV‐BL irradiation source, thus rendering LED‐photocatalysis a sustainable technology for the treatment of persistent contaminants. © 2017 Society of Chemical Industry
Climate change does not impact on Coffea arabica yield in Brazil
Authors: Ferreira Williams PM , Ribeiro Júnior José I , de Fátima Souza Cecília 
Published: June 2017

AbstractBACKGROUNDBrazil is the largest producer of coffee in the world. Studies on climate change estimate large impacts on the production of Coffea arabica (C. arabica). In this context, it is necessary to know the quantitative production values to provide evidence for policy makers to target the prompt answer.RESULTSUsing data from 18 municipalities located in five Brazilian states that produce more coffee in Brazil, in an unprecedented way, in this work it is shown that although the minimum temperature is the most important climatic variable for the production, its effect, although positive, and its degree of explanation, were technically too small to explain the volume of production in Brazilian conditions. According to the model of non‐stationary time series ARIMA (1, 1, 0) coffee production in the future may reach almost four million tons, and the productivity almost 2500 kg ha−1 on average, with the advancement of technology as the main factor that should promote simultaneous increases in production and productivity. However, despite natural climate variations, which make it the most responsible for the variability of annual coffee production, the producer must increase the use of the technologies to support the Brazilian coffee agribusiness.CONCLUSIONSThe results of this study reveal that coffee production in Brazil is due much more to productivity than to the minimum ambient temperature change over the long term; despite this, the climate variable should be considered the most influential on the production and productivity of coffee. © 2017 Embrapa. Journal of the Science of Food and Agriculture © 2019 Society of Chemical Industry.
Polysaccharides, oligosaccharides and nitrogenous compounds change during the ageing of Tempranillo and Verdejo sparkling wines
Authors: Martínez‐Lapuente Leticia , Apolinar‐Valiente Rafael , Guadalupe Zenaida , Ayestarán Belén , Pérez‐Magariño Silvia , Williams Pascale , Doco Thierry 
Published: June 2017

AbstractBACKGROUNDVerdejo and Tempranillo are traditional varieties for producing still wines; however, they could provide an alternative for the manufacturing of sparkling wines. Sparkling wines were elaborated by the traditional method, followed by ageing on lees for 9 months. A study on the changes that take place in polysaccharides, oligosaccharides and nitrogenous compounds during the ageing on lees of Tempranillo and Verdejo sparkling wines has been undertaken.RESULTSMannoproteins and the glucose residue of oligosaccharides were the major carbohydrates detected in all vinification stages. Yeast polysaccharides and glucan‐like structures of the oligosaccharides increased after 3 months of ageing. The evolution of yeast polysaccharides and the composition of PRAG‐like structure were different among grape varieties. A decrease in amino acids and biogenic amines was observed during the ageing. The contents of polysaccharides, oligosaccharides and nitrogenous compound were significantly higher in Tempranillo than in Verdejo sparkling wines at the end of the ageing period.CONCLUSIONPolysaccharides and oligosaccharides from yeast were more significant autolysis markers of sparkling wines than the nitrogenous compounds. Our data suggest a potential cultivar effect on the evolution of yeast polysaccharides and on the composition of PRAG, which may influence the physico‐chemical and sensory properties of sparkling wines. © 2017 Society of Chemical Industry
Yield, quality and biochemical properties of various strawberry cultivars under water stress
Authors: Adak Nafiye , Gubbuk Hamide , Tetik Nedim 
Published: June 2017

AbstractBACKGROUNDAlthough strawberry (Fragaria x ananassa Duch.) species are sensitive to abiotic stress conditions, some cultivars are known to be tolerant to different environmental conditions. We examined the response of different strawberry cultivars to water stress conditions in terms of yield, quality and biochemical features. The trial was conducted under two different irrigation regimes: in grow bags containing cocopeat (control, 30%; water stress, 15% drainage) with four different cultivars (Camarosa, Albion, Amiga and Rubygem).RESULTSFruit weight declined by 59.72% and the yield per unit area by 63.62% under water stress conditions as compared to control. Albion and Rubygem were found to be more tolerant and Amiga the most sensitive in terms of yield under stress conditions. Water stress increased all biochemical features in fruits such as total phenol, total anthocyanin, antioxidant activity and sugar contents. Among the cultivars, glucose and fructose was higher in Albion.CONCLUSIONConsidering the rise in global warming, identification of resistant and tolerant cultivars to stress conditions are crucial for future breeding programmes. Our results showed that some of the fruit's physical features were affected negatively by stress conditions whereas many of the biochemical features such as total anthocyanin content, total phenolic content and antioxidant activity were positively modulated. © 2017 Society of Chemical Industry
Chemical composition and antibacterial, antifungal, allelopathic and acetylcholinesterase inhibitory activities of cassumunar‐ginger
Authors: Verma Ram S , Joshi Neeta , Padalia Rajendra C , Singh Ved R , Goswami Prakash , Verma Sajendra K , Iqbal Hina , Chanda Debabrata , Verma Rajesh K , Darokar Mahendra P , Chauhan Amit , Kandwal Manish K 
Published: June 2017

AbstractBACKGROUNDZingiber montanum (J.Koenig) Link ex A.Dietr. (Zingiberaceae), commonly known as cassumunar‐ginger, is a folk remedy for the treatment of inflammations, sprains, rheumatism and asthma. The aim of the present study was to assess the chemical composition, and antibacterial, antifungal, allelopathic and acetylcholinesterase inhibitory activities of the essential oil of Z. montanum originating from India.RESULTSThe hydrodistilled essential oil of Z. montanum rhizome was analyzed using gas chromatography‐flame ionization detection and gas chromatography–mass spectrometry. A total of 49 constituents, forming 98.7–99.9% of the total oil compositions, was identified. The essential oil was characterized by higher amount of monoterpene hydrocarbons (32.6–43.5%), phenylbutanoids (27.5–41.2%) and oxygenated monoterpenes (11.4–34.1%). Major constituents of the oil were sabinene (13.5–38.0%), (E)‐1‐(3',4'‐dimethoxyphenyl)buta‐1,3‐diene (DMPBD) (20.6–35.3%), terpinen‐4‐ol (9.0–31.3%), γ‐terpinene (1.1–4.8%) and β‐phellandrene (1.0–4.4%). The oil was evaluated against eight pathogenic bacteria and two fungal strains. It exhibited low to good antibacterial activity (minimum inhibitory concentration: 125–500 µg mL–1) and moderate antifungal activity (250 µg mL–1) against the tested strains. The oil reduced germination (69.8%) and inhibited the root and shoot growth of lettuce significantly (LD50: 3.58 µL plate–1). However, it did not demonstrate acetylcholinesterase inhibitory activity up to a concentration of 10 mg mL–1.CONCLUSIONSThe essential oil of Z. montanum can be used as a potential source of DMPBD, terpinen‐4‐ol and sabinene for pharmaceutical products. The results of the present study add significant information to the pharmacological activity of Z. montanum native to India. © 2017 Society of Chemical Industry
Effects of four short‐chain fatty acids or salts on fermentation characteristics and aerobic stability of alfalfa (Medicago sativa L.) silage
Authors: Yuan Xian J , Wen Ai Y , Wang Jian , Desta Seare T , Dong Zhi H , Shao Tao 
Published: June 2017

AbstractBACKGROUNDThe objective of the present study was to evaluate the effects of four chemicals on the fermentation quality and aerobic stability of alfalfa (Medicago sativa L.) silage. Wilted alfalfa was ensiled without additive (control), or with formic acid (FA), potassium diformate (KDF), sodium diacetate (SDA) or calcium propionate (CAP).RESULTSAfter 60 days of ensiling, the pH values in FA, KDF and SDA silages were lower (P < 0.05) compared to that of control and CAP silages, and chemicals (P < 0.05) decreased butyric acid and ammonia N concentrations and populations of aerobic bacteria and yeasts compared to the control. The SDA and CAP silages had a higher (P < 0.05) lactic acid bacteria content compared to the FA and KDF silages. The SDA and CAP silages had higher (P < 0.05) acetic and propionic acid contents compared to the other silages, respectively. The ammonia N concentrations in the FA and KDF silages were lower compared to the other silages during the first 5 days of aerobic exposure, and then increased sharply to 105 and 100 g kg–1 total N, respectively, which was higher (P < 0.05) than that of the SDA and CAP silages on day 9 of aerobic exposure. Yeasts and aerobic bacteria counts in SDA silage slowly increased and remained at lower levels compared to the other silages after 7 days of aerobic exposure.CONCLUSIONAdditives prolonged the aerobic stability duration compared to the control, and the SDA and CAP silages remained stable for more than 216 h, followed by the KDF and FA silages (202 and 196 h, respectively). © 2017 Society of Chemical Industry
Retracted: Early Detection of Postoperative Acute Kidney Injury in Acute Stanford Type A Aortic Dissection With Doppler Renal Resistive Index
Authors: Qin Huai , Wu Haibo , Chen Yi , Zhang Nan , Fan Zhanming 
Published: June 2017

ObjectivesThis study aimed to evaluate the early efficiency of Doppler renal resistive index (DRRI) in prediction of acute kidney injury (AKI) after surgery in acute Stanford Type A aortic dissection (AAAD) patients.MethodsSixty‐one AAAD patients who planned to receive Sun's surgical management were prospectively enrolled. The DRRI was measured by ultrasonography Doppler on the day before surgery (DRRIpre), on admission to the intensive care unit (DRRIT0), 6 hours after surgery (DRRIT6), 24 hours after surgery (DRRIT24), and 48 hours after surgery (DRRIT48). The maximum DRRI value (DRRImax) was recorded. The AKI was evaluated according to the classifications of the Acute Kidney Injury Network. The DRRI and serum creatinine (sCr) were compared between the pre‐ and postoperative time stations, as well as between the AKI and no‐AKI groups.ResultsThirty‐nine (63.9%) patients suffered from AKI, and 12 (19.6%) patients received dialysis. No significant difference was found in DRRIpre (0.63 ± 0.04 versus 0.65 ± 0.06, P = .059) and sCrpre (84.13 ± 23.77 versus 94.29 ± 51.11, P = .383) between the two groups with and without AKI. Both the DRRI and sCr increased significantly after surgery in the AKI groups (P < .001). However, the DRRI reached its maximum 6 hours after surgery, whereas the sCr reached its maximum after 24 hours. Both the DRRI and sCr improved 48 hours after surgery. The area under the receiver operating characteristic curve for DRRImax (0.864, 95% confidence interval: 0.770–0.957) and DRRIT6 (0.861, 95% confidence interval: 0.766‐0.957) was larger than the other three DRRIs measured at different time points. The cutoff value of DRRImax was 0.71, a sensitivity of 76.9% and specificity of 95.5%.ConclusionsPostoperative DRRI predicts the AKI earlier than sCr after AAAD surgery. The best time to detect DRRI was 6 hours after surgery.
Diastolic Dysfunction of Hypertrophic Cardiomyopathy Genotype‐Positive Subjects Without Hypertrophy Is Detected by Tissue Doppler Imaging: A Systematic Review and Meta‐analysis
Authors: Liu Wen , Sun Dandan , Yang Jun 
Published: June 2017

ObjectivesTo evaluate whether diastolic dysfunction derived by tissue Doppler imaging (TDI) would be an earlier manifestation in genotype‐positive hypertrophic cardiomyopathy (HCM) subjects without left ventricular hypertrophy (LVH).MethodsWe systematically searched Pubmed, Medline, and Web of Science with an upper date limit of June 2016 for studies evaluating the diastolic function of HCM genotype‐positive subjects without hypertrophy (G+/LVH−). Based on the inclusion criteria, eligible studies were selected. The quality of selected studies was assessed by the Newcastle–Ottawa Scale before being included in the meta‐analysis. The statistic data such as weighted mean difference (WMD) and 95% confidence interval (CI) were calculated by Stata 12.0 software.ResultsSeventeen studies were included in the systematic review, and 12 were finally involved in the meta‐analysis. The G+/LVH− subjects showed decreased Ea derived by TDI on both the interventricular septum (WMD [95% CI] = –1.822 [–3.104, –0.541]) and lateral wall (WMD [95% CI] = –2.269 [–3.820, –0.719]), and increased E/Ea on both interventricular septum (WMD [95% CI] = 1.363 [0.552, 2.174]) and lateral (WMD [95% CI] = 1.339 [0.386, 2.293]) wall.ConclusionsTissue Doppler imaging–derived diastolic dysfunction can be found in HCM genotype‐positive subjects without hypertrophy.
On the nonlinear dynamic model of open‐type constant flow hydrostatic bearings
Authors: Mao Kuanmin , Du Yikang , Li Bin , Gan Shiyu , Jin Lan 
Published: June 2017

AbstractThis paper presents experimental and theoretical studies used to assess the dynamic performance of open‐type constant flow hydrostatic bearings. A nonlinear dynamic model accounting for the compressibility effect, the squeeze effect, and the hydrostatic effect of hydrostatic fluid for the analytical perdition of the step response is proposed. Moreover, an innovative point‐to‐point contact step excitation setup is designed. In addition, a test rig is developed to measure the step response of the hydrostatic bearings by removing the imposed displacement within 0.0002 second. The experimental results are in good agreement with the theoretical results. The results show that the maximum dynamic displacement is twice the imposed displacement. Furthermore, the settling time of the step response is related to the inlet flow rate, irrespective of the imposed displacement.
Quantitative skin assessment using spatial frequency domain imaging (SFDI) in patients with or at high risk for pressure ulcers
Authors: Yafi Amr , Muakkassa Fuad K. , Pasupneti Tejasvi , Fulton Judy , Cuccia David J. , Mazhar Amaan , Blasiole Kimberly N. , Mostow Eliot N. 
Published: June 2017

Background and ObjectivePressure ulcers (PU) are a significant problem facing the health system in the United States. Here, we present preliminary case studies demonstrating feasibility of Spatial Frequency Domain Imaging (SFDI) to assess skin status in high‐risk populations and pre‐existing wounds. SFDI is a wide‐field non‐contact optical imaging technology that uses structured light to obtain tissue optical properties and of tissue constituents. This study aims to determine the fit of SFDI for PU care and determine the next steps.Study Design/Materials and MethodsPatients at risk for pressure ulcers were imaged using a near‐infrared SFDI system. SFDI‐derived images of tissue function (tissue hemoglobin, tissue oxygen saturation) and structure (tissue scattering) were then compared to each other as well as a blinded dermatologist's clinical impressions.ResultsFour case series were chosen to demonstrate the imaging capability of this technology. The first scenario demonstrates normal skin of three patients without skin breakdown with spatially uniform measures of tissue oxygen saturation, scattering, and blood volume. The second scenario demonstrates a stage II PU; the third case shows non‐blanchable erythema of an unstageable PU; a fourth scenario is a clinically indistinguishable skin rash versus early stages of a PU. In all these cases, we observe spatial changes in tissue constituents (decrease in tissue oxygen saturation, increased blood pooling, decreased scattering).ConclusionWe have presented the first use of SFDI for pressure ulcer imaging and staging. This preliminary study demonstrates the feasibility of this optical technology to assess tissue oxygen saturation and blood volume status in a quantitative manner. With the proposed improvements in modeling and hardware, SFDI has potential to provide a means for pressure ulcer risk stratification, healing and staging. Lasers Surg. Med. 49:827–834, 2017 © 2017 Wiley Periodicals, Inc.