bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thymidylate synthase is functionally associated with ZEB1 and contributes to the epithelial‐to‐mesenchymal transition of cancer cells
Authors: Siddiqui Aarif , Vazakidou Maria Eleni , Schwab Annemarie , Napoli Francesca , Fernandez‐Molina Cristina , Rapa Ida , Stemmler Marc P , Volante Marco , Brabletz Thomas , Ceppi Paolo 
Published: March 2017

AbstractThymidylate synthase (TS) is a fundamental enzyme of nucleotide metabolism and one of the oldest anti‐cancer targets. Beginning from the analysis of gene array data from the NCI‐60 panel of cancer cell lines, we identified a significant correlation at both gene and protein level between TS and the markers of epithelial‐to‐mesenchymal transition (EMT), a developmental process that allows cancer cells to acquire features of aggressiveness, like motility and chemoresistance. TS levels were found to be significantly augmented in mesenchymal‐like compared to epithelial‐like cancer cells, to be regulated by EMT induction, and to negatively correlate with micro‐RNAs (miRNAs) usually expressed in epithelial‐like cells and known to actively suppress EMT. Transfection of EMT‐suppressing miRNAs reduced TS levels, and a specific role for miR‐375 in targeting the TS 3'‐untranslated region was identified. A particularly relevant association was found between TS and the powerful EMT driver ZEB1, the shRNA‐mediated knockdown of which up‐regulated miR‐375 and reduced TS cellular levels. The TS–ZEB1 association was confirmed in clinical specimens from lung tumours and in a genetic mouse model of pancreatic cancer with ZEB1 deletion. Interestingly, TS itself appeared to have a regulatory role in EMT in cancer cells, as TS knockdown could directly reduce the EMT phenotype, the migratory ability of cells, the expression of stem‐like markers, and chemoresistance. Taken together, these data indicate that the TS enzyme is functionally linked with EMT and cancer differentiation, with several potential translational implications. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Extramedullary leukemia in children with acute myeloid leukemia: A population‐based cohort study from the Nordic Society of Pediatric Hematology and Oncology (NOPHO)
Authors: Støve Heidi Kristine , Sandahl Julie Damgaard , Abrahamsson Jonas , Asdahl Peter H. , Forestier Erik , Ha Shau‐Yin , Jahnukainen Kirsi , Jónsson Ólafur G. , Lausen Birgitte , Palle Josefine , Zeller Bernward , Hasle Henrik 
Published: March 2017

AbstractBackgroundThe prognostic significance of extramedullary leukemia (EML) in childhood acute myeloid leukemia is not clarified.ProcedureThis population‐based study included 315 children from the NOPHO‐AML 2004 trial.ResultsAt diagnosis, 73 (23%) patients had EML: 39 (12%) had myeloid sarcoma, 22 (7%) had central nervous system disease, and 12 (4%) had both. EML was associated with young age (median age: 2.6 years), a high white blood cell count (median: 40 × 109/l), M5 morphology (40%), and 11q23/MLL (KMT2A) rearrangements (34%). No patient received involved field radiotherapy. Five‐year event‐free survival did not differ significantly between the EML and the non‐EML patients (54% vs. 45%, P = 0.57), whereas 5‐year overall survival (OS) was significantly lower in the EML group (64% vs. 73%, P = 0.04). The risk of induction death was significantly higher for EML patients (8% vs. 1%, P = 0.002). There was a trend toward a lower risk of relapse for EML patients (5‐year cumulative incidence of relapse 33% vs. 49%, P = 0.16). Traumatic lumbar puncture did not adversely affect survival in this cohort.ConclusionsEML was associated with increased risk of induction death impacting the OS. No patients relapsed at the primary site of the myeloid sarcoma despite management without radiotherapy.
Dual‐responsive crosslinked micelles of a multifunctional graft copolymer for drug delivery applications
Authors: Lin Shudong , Feng Shiting , Mo Yangmiao , Tu Yuanyuan , Guo Yu , Hu Jiwen , Liu Guojun , Zhong Zhiwei , Miao Lei , Zou Hailiang , Liu Feng 
Published: March 2017

ABSTRACTA novel multifunctional amphiphilic graft copolymer has been synthesized consisting of a biodegradable poly(l‐aspartic acid) backbone that was decorated by water‐soluble poly(ethylene glycol) (PEG) and pH‐responsive poly(N,N‐diethylaminoethyl methacrylate) (PDEAEMA) side‐chains as well as thiol pendant groups. This graft copolymer together with doxorubicin (DOX) formed micelles in water at pH = 10.0 with PDEAEMA and DOX acting as the core and PEG serving as the micellar corona. Upon oxidation, the thiol groups dimerized to form disulfide bonds, thus “locking in” the micellar structure. These crosslinked micelles expanded as the pH was decreased from 7.4 to 5.0 or upon the addition, at pH = 7.4, of glutathione (GSH), a thiol‐containing oligopeptide that is present in cancerous cells and cleaves disulfide bonds. At pH = 5.0, GSH addition triggered the disassembly of the micelles. The expansion and disassembly of the micelles have been determined via in vitro experiments to evaluate their DOX release behavior. More importantly, the graft copolymer micelles could enter cells by means of endocytosis and deliver DOX to the nuclei of ovarian cancer BEL‐7402 cells. Thus, this polymer and its micelles are promising candidates for drug delivery applications. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 1536–1546
Mindfulness‐based stress reduction added to care as usual for lung cancer patients and/or their partners: A multicentre randomized controlled trial
Authors: Schellekens M.P.J. , van den Hurk D.G.M. , Prins J.B. , Donders A.R.T. , Molema J. , Dekhuijzen R. , van der Drift M.A. , Speckens A.E.M. 
Published: March 2017

AbstractObjectiveLung cancer patients report among the highest distress rates of all cancer patients. Partners report similar distress rates. The present study examined the effectiveness of additional mindfulness‐based stress reduction (care as usual [CAU] + MBSR) versus solely CAU to reduce psychological distress in lung cancer patients and/or their partners.MethodsWe performed a multicentre, parallel‐group, randomized controlled trial. Mindfulness‐based stress reduction is an 8‐week group‐based intervention, including mindfulness practice and teachings on stress. Care as usual included anticancer treatment, medical consultations, and supportive care. The primary outcome was psychological distress. Secondary outcomes included quality of life, caregiver burden, relationship satisfaction, mindfulness skills, self‐compassion, rumination, and posttraumatic stress symptoms. Outcomes were assessed at baseline, post‐intervention, and 3‐month follow‐up. Linear mixed modeling was conducted on an intention‐to‐treat sample. Moderation (gender, disease stage, baseline distress, participation with/without partner) and mediation analyses were performed.ResultsA total of 31 patients and 21 partners were randomized to CAU + MBSR and 32 patients and 23 partners to CAU. After CAU + MBSR patients reported significantly less psychological distress (p = .008, d = .69) than after CAU. Baseline distress moderated outcome: those with more distress benefitted most from MBSR. Additionally, after CAU + MBSR patients showed more improvements in quality of life, mindfulness skills, self‐compassion, and rumination than after CAU. In partners, no differences were found between groups.ConclusionOur findings suggest that psychological distress in lung cancer patients can be effectively treated with MBSR. No effect was found in partners, possibly because they were more focused on patients' well‐being rather than their own.
Metal–metal bonding in biscycloheptatrienyl dimetal compounds of the second‐row transition metals
Authors: Zhang Zhihui , Feng Xuejun , Chen Qun , He Mingyang , Xie Yaoming , Bruce King R. , Schaefer Henry F. 
Published: March 2017

AbstractDensity functional theory has been used to examine the dimetallocene‐like dicycloheptatrienyl dimetal compounds of the second‐row transition metals (C7H7)2M2 (M = Ru, Tc, Mo, Nb, Zr). The lowest energy (C7H7)2Mo2 structure is a coaxial structure with terminal η7C7H7 rings, whereas the lowest energy (C7H7)2M2 structures (M = Ru, Tc, Nb, Zr) are perpendicular structures with bridging η4,η4C7H7 rings except for the perpendicular (η4,η3C7H7)2Ru2 structure. The metal–metal bond orders in the (C7H7)2M2 structures (M = Ru, Tc, Mo, Nb), as determined by analysis of their frontier molecular orbitals, suggest preferred 16‐ rather than 18‐electron configurations for the central metal atoms. Thus, in the coaxial (η7C7H7)2M2 structures the formal bond orders are two for M = Tc and three for M = Mo. For the perpendicular structures both (η4,η3C7H7)2Ru2 and (η4,η4C7H7)2Tc2 have 16‐electron configurations with metal–metal single bonds owing to the different modes of bonding of the bridging C7H7 rings in the two structures. For the (C7H7)2Zr2 system the perpendicular structure has a formal ZrZr double bond and the coaxial structure has a very long (∼3.5 Å) ZrZr bond indicating only 12‐ to 14‐electron configurations for the zirconium atoms.
Rainwater, soil water, and soil nitrate effects on oxygen isotope ratios of nitrous oxide produced in a green tea (Camellia sinensis) field in Japan
Authors: Zou Yun , Hirono Yuhei , Yanai Yosuke , Hattori Shohei , Toyoda Sakae , Yoshida Naohiro 
Published: March 2017

RationaleThe oxygen exchange fraction between soil H2O and N2O precursors differs in soils depending on the responsible N2O‐producing process: nitrification or denitrification. This study investigated the O‐exchange between soil H2O and N2O precursors in a green tea field with high N2O emissions.MethodsThe rainwater δ18O value was measured using cavity ring‐down spectrometry (CRDS) and compared with that of soil water collected under the tea plant canopy and between tea plant rows. The intramolecular 15N site preference in βNαNO (SP = δ15Nα – δ15Nβ) was determined after measuring the δ15Nα and δ15Nbulk values using gas chromatography/isotope ratio mass spectrometry (GC/IRMS), and the δ18O values of N2O and NO3– were also measured using GC/IRMS.ResultsThe range of δ18O values of rainwater (–11.15‰ to –4.91‰) was wider than that of soil water (–7.94‰ to –5.64‰). The δ18O value of soil water at 50 cm depth was not immediately affected by rainwater. At 10 cm and 20 cm depths of soil between tea plant rows, linear regression analyses of δ18O‐N2O (relative to δ18O‐NO3–) versus δ18O‐H2O (relative to δ18O‐NO3–) yielded slopes of 0.76–0.80 and intercepts of 31–35‰.ConclusionsIn soil between tea plant rows, the fraction of O‐exchange between H2O and N2O precursors was approximately 80%. Assuming that denitrification dominated N2O production, the net 18O‐isotope effect for denitrification (NO3– reduction to N2O) was approximately 31–35‰, reflecting the upland condition of the tea field. Copyright © 2015 John Wiley & Sons, Ltd.
Fragmentation patterns of boron‐dipyrromethene (BODIPY) dyes by electrospray ionization high‐resolution tandem mass spectrometry
Authors: Qi Yulin , Geib Timon , Huynh Anh‐Minh , Jung Gregor , Volmer Dietrich A. 
Published: March 2017

Rationale4,4‐Difluoro‐4‐bora‐3a,4a‐diaza‐s‐indacene derivatives (BODIPYs) are fluorescent organic dyes that are widely used as non‐radioactive labels in biological analyses. The fragmentation behaviour of ten structurally related BODIPYs was studied using tandem mass spectrometry (MS/MS), to support the structural elucidation process during synthesis.MethodsThe BODIPYs were investigated by electrospray ionization (ESI)‐MS/MS, utilizing collision‐induced dissociation (CID) data from triple quadrupole MS and high‐resolution, accurate mass CID data from Fourier transform ion cyclotron resonance (FTICR) experiments.ResultsUnusual radical molecular cations ([M]+•) were formed directly during the ESI process. These radical species dissociated into a large range of product ions during the subsequent CID experiments. Superimposed dissociations originating from parallel [M]+• and [M+H]+ decompositions significantly complicated the interpretation of the MS/MS spectra.ConclusionsDetailed dissociation mechanisms were proposed in this study for BODIPY dyes. The elemental formulae of CID product ions were unambiguously assigned using FTICR‐MS and unique fragment ions were discovered for the rapid identification of methyl, ethyl, butyl, tert‐butyl, and phenyl substituents of individual dyes in BODIPY synthesis mixtures by low‐resolution MS. Copyright © 2015 John Wiley & Sons, Ltd.
The cut‐tree of large trees with small heights
Authors: Berzunza Gabriel 
Published: March 2017

AbstractWe destroy a finite tree of size n by cutting its edges one after the other and in uniform random order. Informally, the associated cut‐tree describes the genealogy of the connected components created by this destruction process. We provide a general criterion for the convergence of the rescaled cut‐tree in the Gromov‐Prohorov topology to an interval endowed with the Euclidean distance and a certain probability measure, when the underlying tree has branching points close to the root and height of order . In particular, we consider uniform random recursive trees, binary search trees, scale‐free random trees and a mixture of regular trees. This yields extensions of a result in Bertoin (Probab Stat 5 (2015), 478–488) for the cut‐tree of uniform random recursive trees and also allows us to generalize some results of Kuba and Panholzer (Online J Anal Combin (2014), 26) on the multiple isolation of vertices. The approach relies in the close relationship between the destruction process and Bernoulli bond percolation, which may be useful for studying the cut‐tree of other classes of trees. © 2017 Wiley Periodicals, Inc. Random Struct. Alg., 51, 404–427, 2017
Turning Potential Into Action: Using Pluripotent Stem Cells to Understand Heart Development and Function in Health and Disease
Authors: Fonoudi Hananeh , Bosman Alexis 
Published: March 2017

Summary
Pluripotent stem cells hold enormous potential for regenerative therapies, however their ability to provide insight into early human development and the origins of disease could arguably provide an even greater outcome. This is primarily due to their contribution to the establishment of a powerful knowledge base of human development, something which all researchers and clinicians can potentially benefit from. Modeling human heart development and disease using pluripotent stem cells has already provided many important insights into cardiogenesis and cardiovascular disease mechanisms however, it is important to be aware of the complexities of this model system. Thorough contemplation of experimental models and specialized techniques is required to provide high-quality evidence of the intricacies of both normal early development, and when this process goes awry in disease states.
One‐Step Synthesis of Fused Chromeno[4,3‐b]pyrrolo[3,2‐h]quinolin‐7(1H)‐One Compounds and their Anticancer Activity Evaluation
Authors: Thigulla Yadagiri , Kumar T. Uday , Trivedi Prakruti , Ghosh Balaram , Bhattacharya Anupam 
Published: March 2017

AbstractFeCl3 catalyzed synthesis of chromeno[4,3‐b]pyrrolo[3,2‐h]quinolin‐7(1H)‐one compounds is reported in this paper. The target molecules were synthesized by combining 4‐hydroxy coumarin, 7‐ amino indole and diverse aromatic aldehydes in 1,4‐dioxane as a solvent, at 90 °C. The final compounds obtained were subjected to anticancer activity evaluation using murine melanoma cell lines (B16F10). Best result was obtained for compound bearing 2‐chloro‐3‐pyridyl substituent, which showed IC50 value of 70.74 μM.
Direct Chiral Separation of Binaphthyl Derivatives Using Atroposelective Antibodies
Authors: Adachi Takuma , Odaka Tomoki , Harada Akira , Yamaguchi Hiroyasu 
Published: March 2017

AbstractA simple procedure for chiral separation based on the difference in the molecular weights of the antigen‐antibody complex and analyte is developed. We raise monoclonal antibodies (mAbs) highly specific for the R‐ or S‐isomer of a binaphthyl derivative (BN) by immunizing mice with the corresponding BN enantiomer. Aqueous solutions of racemic BN are mixed with mAbs. The free BN is separated by ultrafiltration. The enantiomers of BN are obtained with a high e.e. using atroposelective antibodies.
Ionic Liquid [HMIm]BF4 Mediated and Promoted Eco‐Friendly One‐Pot Sequential Synthesis of New Isoxazolyl Quinazolin‐4(3H)‐ones
Authors: Pittala Praveen Kumar , Eligeti Rajanarendar , Modugu Nagi Reddy 
Published: March 2017

Abstract1‐Methyl imidazolium tetraflouroborate ([HMIm]BF4) has been discovered to be an effective eco‐friendly solvent cum activator for a novel one‐pot sequential coupling and oxidative cyclization of 4‐amino‐3‐methyl‐5‐styrylisoxazoles, 2‐aminobenzoyl chloride and benzyl alcohols to produce new isoxazolyl quinazolin‐4(3H)‐ones under ambient conditions. The protocol proves to be an efficient and an environmentally benign in terms of high yields, low reaction times, ease of recovery, and reusability of reaction medium.
Fabrication of H‐TiO2/CdS/Cu2‐xS Ternary Heterostructures for Enhanced Photocatalytic Hydrogen Production
Authors: Chu Jiayu , Han Xijiang , Yu Zhen , Du Yunchen , Song Bo , Xu Ping 
Published: March 2017

AbstractSolar‐driven photocatalytic water splitting is a promising technology to produce H2 energy. Herein, we demonstrate the fabrication of a ternary catalyst, H‐TiO2/CdS/Cu2‐xS, by chemical bath deposition and ion exchange for efficient photocatalytic water splitting, based on hydrogenated TiO2 (H‐TiO2) with roughened surface and hollow structures. The as‐prepared H‐TiO2/CdS/Cu2‐xS ternary structure can make use of the full solar spectrum and have a better response to the near infrared region. The tuned bandgap alignments in this ternary heterostructure enable efficient separation of photogenerated electrons and holes, leading to a hydrogen production rate as high as 261.54 μmol⋅g−1⋅h−1 without noble metal as co‐catalyst. Meanwhile, a photocatalytic hydrogen production rate of 210.63 μmol⋅g−1⋅h−1 can be maintained after five cycles. We believe this fabricated noble metal‐free ternary heterostructure catalyst has a great potential in solar energy conversion with improved solar energy absorption and efficient electron‐hole separation.
Coadapting multidimension process properties
Authors: Khelladi Djamel Eddine , Bendraou Reda , Hebig Regina , Gervais Marie‐Pierre 
Published: March 2017

AbstractIn the last decades, process verification has been intensively addressed and has become an essential activity to correct and to remove errors before process execution. Typical process verification ecosystems propose to express properties to be verified on the process. A property expresses a desired behavior that must hold or not in the process execution. Processes during their lifespan are continuously adapted for several purposes: enriching, correcting, and refactoring the process. When a process is adapted, the existing properties must naturally be rechecked to ensure that no errors have been introduced, ie, the properties still hold. However, the properties may become outdated and must be coadapted w.r.t. the adapted process before to be rechecked. Otherwise, the verification may raise false alarms or may not detect newly introduced errors. In this paper, we propose a coadaptation approach of properties while considering process adaptation for the different dimensions, namely, control flow, object flow, resources, and timing. We systematically studied process changes in the multiple dimensions to identify those that do impact properties and for which we propose resolution strategies. Our preliminary evaluation shows that our resolutions strategies allow to support users in correctly coadapting impacted properties.
Longitudinal volumetric and 2D assessment of cerebellar atrophy in a large cohort of children with phosphomannomutase deficiency (PMM2‐CDG)
Authors: de Diego Víctor , Martínez‐Monseny Antonio F. , Muchart Jordi , Cuadras Daniel , Montero Raquel , Artuch Rafael , Pérez‐Cerdá Celia , Pérez Belén , Pérez‐Dueñas Belén , Poretti Andrea , Serrano Mercedes ,   Collaborators of the CDG Spanish‐Consortium
Published: March 2017

AbstractObjectiveWe aim to delineate the progression of cerebellar atrophy (the primary neuroimaging finding) in children with phosphomannomutase‐deficiency (PMM2‐CDG) by analyzing longitudinal MRI studies and performing cerebellar volumetric analysis and a 2D cerebellar measurement.MethodsStatistical analysis was used to compare MRI measurements [midsagittal vermis relative diameter (MVRD) and volume] of children with PMM2‐CDG and sex‐ and age‐matched controls, and to determine the rate of progression of cerebellar atrophy at different ages.ResultsFifty MRI studies of 33 PMM2‐CDG patients were used for 2D evaluation, and 19 MRI studies were available for volumetric analysis. Results from a linear regression model showed that patients have a significantly lower MVRD and cerebellar volume compared to controls (p < 0.001 and p < 0.001 respectively). There was a significant negative correlation between age and MVRD for patients (p = 0.014). The rate of cerebellar atrophy measured by the loss of MVRD and cerebellar volume per year was higher at early ages (r = −0.578, p = 0.012 and r = −0.323, p = 0.48 respectively), particularly in patients under 11 years (p = 0.004). There was a significant positive correlation between MVRD and cerebellar volume in PMM2‐CDG patients (r = 0.669, p = 0.001).ConclusionsOur study quantifies a progression of cerebellar atrophy in PMM2‐CDG patients, particularly during the first decade of life, and suggests a simple and reliable measure, the MVRD, to monitor cerebellar atrophy. Quantitative measurement of MVRD and cerebellar volume are essential for correlation with phenotype and outcome, natural follow‐up, and monitoring in view of potential therapies in children with PMM2‐CDG.
Thermophysical Properties of Liquid Aluminum
Authors: Leitner Matthias , Leitner Thomas , Schmon Alexander , Aziz Kirmanj , Pottlacher Gernot 
Published: March 2017

Abstract
Ohmic pulse-heating with sub-microsecond time resolution is used to obtain thermophysical properties for aluminum in the liquid phase. Measurement of current through the sample, voltage drop across the sample, surface radiation, and volume expansion allow the calculation of specific heat capacity and the temperature dependencies of electrical resistivity, enthalpy, and density of the sample at melting and in the liquid phase. Thermal conductivity and thermal diffusivity as a function of temperature are estimated from resistivity data using the Wiedemann–Franz law. Data for liquid aluminum obtained by pulse-heating are quite rare because of the low melting temperature of aluminum with 933.47 K (660.32 °C), as the fast operating pyrometers used for the pulse-heating technique with rise times of about 100 ns generally might not be able to resolve the melting plateau of aluminum because they are not sensitive enough for such low temperature ranges. To overcome this obstacle, we constructed a new, fast pyrometer sensitive in this temperature region. Electromagnetic levitation, as the second experimental approach used, delivers data for surface tension (this quantity is not available by means of the pulse-heating technique) and for density of aluminum as a function of temperature. Data obtained will be extensively compared to existing literature data.