bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing Research in Teacher Education for Sustainability: UN DESD via the Journal of Teacher Education for Sustainability
Authors: Pipere Anita , Veisson Marika , Salīte Ilga 
Published: December 2015

Abstract
This study marks the end of the UN Decade of Education for Sustainable Development that coincides with the 10th anniversary of the Baltic and Black Sea Circle Consortium on Educational Research (BBCC), and aims to analyse the research output performance of BBCC members and other scholars published in the Journal of Teacher Education for Sustainability (JTEFS) during this last decade. Using the methodology of bibliometric study and literature review, the authors describe the main bibliographic indicators of JTEFS and provide a qualitative and quantitative analysis of the research paradigms and their developmental changes in the papers published by JTEFS (2005ñ2014). The results of the study show that in general the journal has evolved in line with the advanced trends in educational research, research in teacher education, research in sustainability education and sustainability studies in higher education. The analysis of published papers indicates both the progress and issues of research in teacher education for sustainability. The authors conclude with some visionary insights into the further development of JTEFS and this research field in general.
Innovative and Traditional Elements in the Work of Academic Staff: The Views of Pre-service Teachers
Authors: Jurgena Inese , Cēdere Dagnija , Keviša Ingrīda 
Published: December 2015

Abstract
The academic staff of the institutions of higher education plays a key role in the implementation of innovations in the study process. This article aims to analyze the views of students, pre-service teachers, on the role of innovations and traditions in the work of the academic staff at their institution of higher education. The survey data from 192 full-time and part-time first year students at Riga Teacher Training and Educational Management Academy show that readiness for innovative activity and active involvement in the creation, acquisition and implementation of innovations is an essential factor in the work of the academic staff. The results indicate that according to the views of pre-service teachers, in the context of pedagogy, the term ‘innovation’ refers to the process of renewal, educational reforms, selection of the most appropriate teaching material, and the elaboration, adoption and utilization of innovations in the pedagogical practice.
Human Securitability: A Participatory Action Research Study Involving Novice Teachers and Youngsters
Authors: Kravale-Pauliņa Mārīte , Oļehnoviča Eridiana 
Published: December 2015

Abstract
Civic participation, initiative and interest in current events can bridge the alienation felt towards national and municipal institutions, thereby enabling individuals to improve their quality of life and contribute to all-round sustainable development of their resident state. This paper reports on a participatory action research study into civic initiatives for securitability involving novice teachers and youngsters from the Latgale region of Latvia. Research participants evaluated national planning documents, enhanced their knowledge and devised civic initiatives to improve the quality of life. Focus group discussions and reasoned argumentative essays were employed to establish how novice teachers (n = 40) and youngsters (n = 58) make sense of the concept of ‘human securitability’. Data analysis was accomplished by qualitative content analysis. The action research exposed an initial understanding of novice teachers and youngsters regarding the human securitability and the possibilities of improving the quality of life. Moreover, this study provided an environment for the research participants to deepen their understanding of said phenomena and participate in educational events envisioning practical engagement with securitability and civic initiatives. The action research study created initiatives for the development of civic securitability and the participation in setting developmental goals.
Ignition Study on a Rotary-valved Air-breathing Pulse Detonation Engine
Authors: Wu Yuwen , Han Qixiang , Shen Yujia , Zhao Wei 
Published: December 2015

AbstractIn the present study, the ignition effect on detonation initiation was investigated in the air-breathing pulse detonation engine. Two kinds of fuel injection and ignition methods were applied. For one method, fuel and air was pre-mixed outside the PDE and then injected into the detonation tube. The droplet sizes of mixtures were measured. An annular cavity was used as the ignition section. For the other method, fuel-air mixtures were mixed inside the PDE, and a pre-combustor was utilized as the ignition source. At firing frequency of 20 Hz, transition to detonation was obtained. Experimental results indicated that the ignition position and initial flame acceleration had important effects on the deflagration-to-detonation transition.
Genome-Wide Mutational Signature of the Chemotherapeutic Agent Mitomycin C inCaenorhabditis elegans
Authors: Tam Annie S , Chu Jeffrey S C , Rose Ann M 
Published: December 2015

AbstractCancer therapy largely depends on chemotherapeutic agents that generate DNA lesions. However, our understanding of the nature of the resulting lesions as well as the mutational profiles of these chemotherapeutic agents is limited. Among these lesions, DNA interstrand crosslinks are among the more toxic types of DNA damage. Here, we have characterized the mutational spectrum of the commonly used DNA interstrand crosslinking agent mitomycin C (MMC). Using a combination of genetic mapping, whole genome sequencing, and genomic analysis, we have identified and confirmed several genomic lesions linked to MMC-induced DNA damage in Caenorhabditis elegans. Our data indicate that MMC predominantly causes deletions, with a 5′-CpG-3′ sequence context prevalent in the deleted regions of DNA. Furthermore, we identified microhomology flanking the deletion junctions, indicative of DNA repair via nonhomologous end joining. Based on these results, we propose a general repair mechanism that is likely to be involved in the biological response to this highly toxic agent. In conclusion, the systematic study we have described provides insight into potential sequence specificity of MMC with DNA.
Genome-Wide Estimates of Mutation Rates and Spectrum inSchizosaccharomyces pombeIndicate CpG Sites are Highly Mutagenic Despite the Absence of DNA Methylation
Authors: Behringer Megan G , Hall David W 
Published: December 2015

AbstractWe accumulated mutations for 1952 generations in 79 initially identical, haploid lines of the fission yeast Schizosaccharomyces pombe, and then performed whole-genome sequencing to determine the mutation rates and spectrum. We captured 696 spontaneous mutations across the 79 mutation accumulation (MA) lines. We compared the mutation spectrum and rate to a recently published equivalent experiment on the same species, and to another model ascomycetous yeast, the budding yeast Saccharomyces cerevisiae. While the two species are approximately 600 million years diverged from each other, they share similar life histories, genome size and genomic G/C content. We found that Sc. pombe and S. cerevisiae have similar mutation rates, but Sc. pombe exhibits a stronger insertion bias. Intriguingly, we observed an increased mutation rate at cytosine nucleotides, specifically CpG nucleotides, which is also seen in S. cerevisiae. However, the absence of methylation in Sc. pombe and the pattern of mutation at these sites, primarily C → A as opposed to C → T, strongly suggest that the increased mutation rate is not caused by deamination of methylated cytosines. This result implies that the high mutability of CpG dinucleotides in other species may be caused in part by a methylation-independent mechanism. Many of our findings mirror those seen in the recent study, despite the use of different passaging conditions, indicating that MA is a reliable method for estimating mutation rates and spectra.
Identification of a Novel Nematotoxic Protein by Challenging the Model Mushroom Coprinopsis cinerea with a Fungivorous Nematode
Authors: Plaza David Fernando , Schmieder Stefanie Sofia , Lipzen Anna , Lindquist Erika , Künzler Markus 
Published: December 2015

Abstract
The dung of herbivores, the natural habitat of the model mushroom Coprinopsis cinerea, is a nutrient-rich but also very competitive environment for a saprophytic fungus. We showed previously that C. cinerea expresses constitutive, tissue-specific armories against antagonists such as animal predators and bacterial competitors. In order to dissect the inducible armories against such antagonists, we sequenced the poly(A)-positive transcriptome of C. cinerea vegetative mycelium upon challenge with fungivorous and bacterivorous nematodes, Gram-negative and Gram-positive bacteria and mechanical damage. As a response to the fungivorous nematode Aphelenchus avenae, C. cinerea was found to specifically induce the transcription of several genes encoding previously characterized nematotoxic lectins. In addition, a previously not characterized gene encoding a cytoplasmic protein with several predicted Ricin B-fold domains, was found to be strongly upregulated under this condition. Functional analysis of the recombinant protein revealed a high toxicity toward the bacterivorous nematode Caenorhabditis elegans. Challenge of the mycelium with A. avenae also lead to the induction of several genes encoding putative antibacterial proteins. Some of these genes were also induced upon challenge of the mycelium with the bacteria Escherichia coli and Bacillus subtilis. These results suggest that fungi have the ability to induce specific innate defense responses similar to plants and animals.
On the Consequences of Purging and Linkage on Fitness and Genetic Diversity
Authors: Bersabé Diego , Caballero Armando , Pérez-Figueroa Andrés , García-Dorado Aurora 
Published: December 2015

AbstractUsing computer simulation we explore the consequences of linkage on the inbreeding load of an equilibrium population, and on the efficiency of purging and the loss of genetic diversity after a reduction in population size. We find that linkage tends to cause increased inbreeding load due to the build up of coupling groups of (partially) recessive deleterious alleles. It also induces associative overdominance at neutral sites but rarely causes increased neutral genetic diversity in equilibrium populations. After a reduction in population size, linkage can cause some delay both for the expression of the inbreeding load and the corresponding purging. However, reasonable predictions can be obtained for the evolution of fitness under inbreeding and purging by using empirical estimates of the inbreeding depression rate. Purging selection against homozygotes for deleterious alleles affects the population’s pedigree. Furthermore, it can slow the loss of genetic diversity compared to that expected from the variance of gametic contributions to the breeding group and even from pedigree inbreeding. Under some conditions, this can lead to a smaller loss of genetic diversity, even below that expected from population size in the absence of selection.
Genomic Analysis of ATP Efflux in Saccharomyces cerevisiae
Authors: Peters Theodore W , Miller Aaron W , Tourette Cendrine , Agren Hannah , Hubbard Alan , Hughes Robert E 
Published: December 2015

Abstract
Adenosine triphosphate (ATP) plays an important role as a primary molecule for the transfer of chemical energy to drive biological processes. ATP also functions as an extracellular signaling molecule in a diverse array of eukaryotic taxa in a conserved process known as purinergic signaling. Given the important roles of extracellular ATP in cell signaling, we sought to comprehensively elucidate the pathways and mechanisms governing ATP efflux from eukaryotic cells. Here, we present results of a genomic analysis of ATP efflux from Saccharomyces cerevisiae by measuring extracellular ATP levels in cultures of 4609 deletion mutants. This screen revealed key cellular processes that regulate extracellular ATP levels, including mitochondrial translation and vesicle sorting in the late endosome, indicating that ATP production and transport through vesicles are required for efflux. We also observed evidence for altered ATP efflux in strains deleted for genes involved in amino acid signaling, and mitochondrial retrograde signaling. Based on these results, we propose a model in which the retrograde signaling pathway potentiates amino acid signaling to promote mitochondrial respiration. This study advances our understanding of the mechanism of ATP secretion in eukaryotes and implicates TOR complex 1 (TORC1) and nutrient signaling pathways in the regulation of ATP efflux. These results will facilitate analysis of ATP efflux mechanisms in higher eukaryotes.
Identification of a Classical Mutant in the Industrial HostAspergillus nigerby Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites
Authors: Niu Jing , Arentshorst Mark , Nair P Deepa S , Dai Ziyu , Baker Scott E , Frisvad Jens C , Nielsen Kristian F , Punt Peter J , Ram Arthur F J 
Published: December 2015

AbstractThe asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme production. Physiological analysis showed that this mutant did not secrete large amounts of citric acid and oxalic acid, thus explaining the nonacidifying phenotype. As traditional complementation approaches to characterize the mutant genotype were unsuccessful, we used bulk segregant analysis in combination with high-throughput genome sequencing to identify the mutation responsible for the nonacidifying phenotype. Since A. niger has no sexual cycle, parasexual genetics was used to generate haploid segregants derived from diploids by loss of whole chromosomes. We found that the nonacidifying phenotype was caused by a point mutation in the laeA gene. LaeA encodes a putative methyltransferase-domain protein, which we show here to be required for citric acid production in an A. niger lab strain (N402) and in other citric acid production strains. The unexpected link between LaeA and citric acid production could provide new insights into the transcriptional control mechanisms related to citric acid production in A. niger. Interestingly, the secondary metabolite profile of a ΔlaeA strain differed from the wild-type strain, showing both decreased and increased metabolite levels, indicating that LaeA is also involved in regulating the production of secondary metabolites. Finally, we show that our systems genetics approach is a powerful tool to identify trait mutations.
Chromoanasynthetic Genomic Rearrangement Identified in a N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Screen in Caenorhabditis elegans
Authors: Itani Omar A , Flibotte Stephane , Dumas Kathleen J , Moerman Donald G , Hu Patrick J 
Published: December 2015

Abstract
Chromoanasynthesis is a recently discovered phenomenon in humans with congenital diseases that is characterized by complex genomic rearrangements (CGRs) resulting from aberrant repair of catastrophic chromosomal damage. How these CGRs are induced is not known. Here, we describe the structure and function of dpDp667, a causative CGR that emerged from a Caenorhabditis elegans dauer suppressor screen in which animals were treated with the point mutagen N-ethyl-N-nitrosourea (ENU). dpDp667 comprises nearly 3 Mb of sequence on the right arm of the X chromosome, contains three duplications and one triplication, and is devoid of deletions. Sequences from three out of the four breakpoint junctions in dpDp667 reveal microhomologies that are hallmarks of chromoanasynthetic CGRs. Our findings suggest that environmental insults and physiological processes that cause point mutations may give rise to chromoanasynthetic rearrangements associated with congenital disease. The relatively subtle phenotype of animals harboring dpDp667 suggests that the prevalence of CGRs in the genomes of mutant and/or phenotypically unremarkable animals may be grossly underestimated.
Comprehensive Transcriptome Analysis of Six Catfish Species from an Altitude Gradient Reveals Adaptive Evolution in Tibetan Fishes
Authors: Ma Xiuhui , Dai Wei , Kang Jingliang , Yang Liandong , He Shunping 
Published: December 2015

Abstract
Glyptosternoid fishes (Siluriformes), one of the three broad fish lineages (the two other are schizothoracines and Triplophysa), have a limited distribution in the rivers in the Tibetan Plateau and peripheral regions. To investigate the genetic mechanisms underlying adaptation to the Tibetan Plateau in several fish species from gradient altitudes, a total of 20,659,183–37,166,756 sequence reads from six species of catfish were generated by Illumina sequencing, resulting in six assemblies. Analysis of the 1,656 orthologs among the six assembled catfish unigene sets provided consistent evidence for genome-wide accelerated evolution in the three glyptosternoid lineages living at high altitudes. A large number of genes refer to functional categories related to hypoxia and energy metabolism exhibited rapid evolution in the glyptosternoid lineages relative to yellowhead catfish living in plains areas. Genes showing signatures of rapid evolution and positive selection in the glyptosternoid lineages were also enriched in functions associated with energy metabolism and hypoxia. Our analyses provide novel insights into highland adaptation in fishes and can serve as a foundation for future studies aiming to identify candidate genes underlying the genetic basis of adaptation in Tibetan fishes.
The Erotic as a Liberating and Empowering Force in Two Costa Rican Female Poets: Julieta Dobles and Ana Istarú
Authors: Barquero Vargas Ana Patricia 
Published: December 2015

<p> Este artículo explora la poesía erótica de dos escritoras costarricenses, Julieta Dobles y Ana Istarú, como una fuerza liberadora y de empoderamiento. Propone que estas poetas transgreden el discurso hegemónico patriarcal por medio del erotismo, haciendo así posible no solamente su liberación y empoderamiento, sino también el de muchas mujeres quienes se ven reflejadas en su poesía. Su poesía corporal abre una perspectiva donde ellas puedan empezar a escudriñar sus deseos sexuales y sus cuerpos, al tomar su sexo literalmente en sus propias manos.</p>
Assertiveness in public administration officials of Montería, Colombia/Asertividad en funcionarios de la administración pública del municipio de Montería, Colombia
Authors: Ruiz Arias Viviana María , Nobles Montoya Diana Victoria , Ruiz Otero Carlos Eduardo 
Published: December 2015

En el presente artículo de investigación se muestran los resultados del estudio “Grado de asertividad de los funcionarios de atención al cliente de la administración pública del municipio de Montería”, Colombia. El objetivo fue evaluar el estilo de asertividad que los funcionarios emplean para enfrentar las diversas situaciones sociales, responder a la crítica y resolver problemas. La población estuvo constituida por quince (15) funcionarios entre los 30 y 55 años de edad quienes laboran en las dependencias de recursos humanos, Secretaria de hacienda y Sisben. El diseño metodológico fue descriptivo, transversal con un enfoque cuantitativo. El instrumento de evaluación que se utilizó fue la Escala multidimensional de asertividad. Los resultados obtenidos evidencian un déficit en conductas asertivas en los funcionarios
Creation of a learning community: an experience of inclusive education in Colombia/Creación de una comunidad de aprendizaje: una experiencia de educación inclusiva en Colombia
Authors: Beltrán Villamizar Yolima Ivonne , Martínez Fuentes Yexica Lizeth , Torrado Duarte Omar Elías 
Published: December 2015

El objetivo del trabajo fue articular el esfuerzo de profesores, estudiantes, padres de familia y otros miembros de la comunidad alrededor de actividades de mejoramiento de la calidad educativa en una institución educativa colombiana, situada en un entorno social y cultural que dificulta la prestación del servicio educativo. Teóricamente se asumió el concepto de “comunidades de aprendizaje” entendidas como proyectos de transformación social y cultural de un centro educativo y de su entorno, basados en el aprendizaje dialógico, con la finalidad de vincular a toda la comunidad al proceso educativo en espacios concretos, incluyendo el aula de clases. Metodológicamente se procedió a través de la investigación acción siguiendo las siete fases metodológicas para la transformación de centros educativos a “comunidades de aprendizaje-CA, a saber: sensibilización, toma de decisiones, sueño, análisis contextual y selección de prioridades, planificación de aspectos a transformar, formación de familiares y grupos interactivos. Los resultados muestran que las estrategias utilizadas permitieron identificar las principales necesidades en la institución, propiciar un proceso participativo de la comunidad educativa, y fortalecer la relación entre el saber cultural de toda la comunidad y el saber académico.
Public policies on citizen coexistence: Challenges and opportunities/Políticas públicas en materia de convivencia ciudadana: Retos y posibilidades
Authors: Rujano Roque Raima , Salas Johel Jesús 
Published: December 2015

El artículo de investigación, resultado de un estudio realizado en dos comunidades ubicadas al norte del municipio Maracaibo: Santa Rosa de Agua y Monte Bello. Ambas socioeconómicamente diferenciadas, diserta sobre los retos y las posibilidades que tienen las instancias gubernamentales para el diseño e implementación de políticas públicas para el desarrollo de una convivencia ciudadana participativa. Se investigó bajo la modalidad empírica-inductiva en su fase descriptiva. Se aplicó un procedimiento muestral de tipo probabilístico. Entre los resultados destaca que existe un alto nivel de disposición compartida por parte de los habitantes de ambas comunidades para trabajar por el bienestar colectivo. Sin embargo, existe una notable diferencia en las formas en las que los vecinos desean participar. Estas formas diversas constituyen un elemento que fortalece la convivencia ciudadana y a la vez es un elemento que podría facilitar el diseño de estrategias que la promuevan. Como actores clave para trabajar en el fortalecimiento, se mencionan: los consejos comunales y los cuerpos policiales. Como estrategias, se señalan las actividades deportivas, culturales y el asistir a las personas en situaciones de vulnerabilidad. Finalmente, suscriben que dichas estrategias deben implementarse en los espacios públicos, instituciones educativas y religiosas presentes en ambos sectores.
Synthesis and Characterization of Zeolite Y From Bagasse Ash with Hydrothermal Temperatures Variations Using The Sol-Gel Method
Authors: Ali Soifi , Amalia Suci , Fasya Ahmad Ghanaim 
Published: December 2015

<p class="Abstract">Zeolite Synthesis Y is done using sol-gel method on the molar composition of 10Na<sub>2</sub>O: 15 SiO<sub>2</sub>: x Al<sub>2</sub>O<sub>3</sub>: 300 H<sub>2</sub>O with Si/Al on ratio of 2,43 and hydrothermal temperatures variations of 60, 80 and 100 °C. Characterization includes XRD to discover crystallinity and the purity of zeolite, FTIR for the analysis of functional groups and of the surface width with the adsorption of methylene blue. XRD result shows that the formed zeolites Y is still mixed with zeolite P, the best result in synthesis with hydrothermal temperatures of 80 °C. FTIR analysis showsthat all zeolite synthesis contain a common functionO-Si-O/O-Al-O and double ring. The surface area of zeolite Y at 60, 80 and 100 °C is 22,5522; 23,0603 and22,9898m<sup>2</sup>/gram.<strong></strong></p><p class="BodyAbstract"><strong> </strong></p><p class="BodyAbstract"><em><strong>Keywords</strong><strong>:</strong> Bagasse, sol-gel, temperature hydrothermal, zeolite Y</em></p>