bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stochastic processes drive the dynamic assembly of bacterial communities in Salix matsudana afforested soils
Authors: Wang Can , Masoudi Abolfazl , Wang Min , Wang Yin , Zhang Ze , Cao Jingkun , Feng Jian , Yu Zhijun , Liu Jingze 
Published: September 2024

IntroductionThis study investigates the dynamic shifts in soil bacterial communities within a Salix matsudana afforested ecosystem transitioning from agricultural land. Understanding the temporal variability in bacterial diversity and community structures is crucial for informing forest management and conservation strategies, particularly in regions undergoing afforestation.MethodsWe employed high-throughput sequencing across three distinct months (August, September, and October) to analyze the temporal variability in bacterial community composition and diversity. Network analysis was utilized to identify keystone species and assess community stability under varying environmental conditions, including fluctuations in temperature and precipitation.ResultsWe uncover significant temporal variability in bacterial diversity and community structures, which are closely tied to fluctuations in temperature and precipitation. Our findings reveal the abundance of the dominant bacterial phyla, such as Actinobacteria and Proteobacteria, which did not change overall, highlighting the stability and resilience of the microbial community across seasonal transitions. Notably, the increasing similarity in community composition from August to October indicates a reduction in species turnover, likely driven by more homogeneous environmental conditions. Through comprehensive network analysis, we identify the pivotal role of keystone species, particularly the human pathogen Nocardia, in maintaining community stability under reduced soil moisture. The observed variations in community connectivity underscore the microbial community’s resilience and adaptability to seasonal shifts, with higher stability in August and October contrasting with the instability observed in September.DiscussionThese results underscore the complex interplay between stochastic and deterministic processes in bacterial community assembly, significantly shaped by prevailing environmental conditions. The insights gained from this research have far-reaching implications for forestry management and conservation strategies, particularly in regions undergoing similar afforestation efforts.
Comparative analysis of PD-L1 expression and molecular alterations in primary versus metastatic lung adenocarcinoma: a real-world study in China
Authors: Chen Gang , Yu Yang , Qi Youchao , Li Guangxu , Li Ning , Meng Fande , Wang Wujie , Shen Rong 
Published: September 2024

ObjectivesProgrammed death-ligand 1 (PD-L1) is the only Food and Drug Administration-approved biomarker for monitoring response to immune checkpoint inhibitor (ICI) therapy in patients with lung adenocarcinoma. Understanding the nuances of molecular phenotypes, clinical attributes, and PD-L1 expression levels in primary and metastatic lung adenocarcinoma may help predict response to therapy and assist in the clinical management of lung adenocarcinoma.MethodsA total of 235 primary and metastatic lesion specimens from patients with non-small cell lung cancer (NSCLC) an institution in Shandong, China were analyzed. PD-L1 expression was assessed by immunohistochemistry using the 22C3 antibody, and the molecular phenotype was determined by next-generation sequencing of 450 genes. The molecular phenotypes of the primary and metastatic lesions were compared.ResultsElevated PD-L1 expression was significantly associated with advanced and metastatic disease (P = 0.001). The distribution of PD-L1 expression varied based on the anatomical location, showing a higher frequency of elevated PD-L1 expression in distal metastases than in the primary tumor. Metastatic lesions exhibited a higher proportion of carcinogenic pathway gene alterations and a greater number of DNA damage-repair pathway gene alterations than the primary lesions. Notably, CDKN2A copy number deletions were more prevalent in metastatic lesions than in primary lesions. Clinical data stemming from research conducted at the Memorial Sloan Kettering Cancer Center revealed an association between the absence of CDKN2A expression and a poorer prognosis in stage I lung adenocarcinoma.ConclusionSamples of metastatic tumors exhibited a higher proportion of elevated PD-L1 expression, a greater number of pathway alterations, and a higher occurrence of CDKN2A copy number deletions than primary samples. This highlights the importance of reinforcing the clinical management and follow-up of patients with CDKN2A deficiency, particularly within the subset of stage I lung adenocarcinoma.
Functions of patient- and family-centered pediatric cancer communication in Pakistan
Authors: Graetz Dylan E. , Ahmad Alia , Raza Muhammad Rafie , Hameed Ambreen , Naheed Asma , Najmi Atoofa , Quanita Afia tul , Munir Shabnam , Ahmad Safwan , Ferrara Gia , Staples Courtney , Rodriguez Galindo Carlos , Ahmer Hamid Syed , Jeha Sima , Mack Jennifer W. 
Published: September 2024

BackgroundCommunication is an essential aspect of high-quality patient- and family-centered care. A model for pediatric cancer communication developed in the United States defined eight communication functions. The purpose of this study was to explore the relevance of these functions in Pakistan as part of an effort to understand the role of culture in communication.Materials and methodsSemi-structured interviews were conducted with 20 clinicians and 18 caregivers of children with cancer at two major cancer centers. Interviews were conducted in Urdu or English and transcribed and translated as necessary. Two independent coders used a priori codes related to the communication model as well as novel codes derived inductively. Thematic analysis focused on operationalization of the functional communication model.ResultsClinicians and caregivers in Pakistan discussed the importance of all eight communication functions previously identified including: information exchange, decision-making, managing uncertainty, enabling family self-management, responding to emotions, supporting hope, providing validation, and building relationships. The operationalization of these functions was influenced by Pakistani cultural context. For example, information-exchange included the importance of addressing preconceptions and community myths, while managing uncertainty included strong references to religion and faith-based coping. Essential to all eight functions was trust between the family and the medical team.DiscussionThese findings support the use of this functional communication model in diverse pediatric oncology settings and emphasize the importance of trust. Culturally sensitive operationalization of these functions could inform the adaptation of tools to measure communication and interventions aimed at supporting the needs of parents of children with cancer.
Outcomes and toxicities in patients with diffuse-large B cell lymphoma involving the gastrointestinal tract and digestive organs
Authors: Manzar Gohar S. , Cha Elaine E. , Corrigan Kelsey L. , Yoder Alison K. , Schrank Benjamin R. , Nasr Lewis F. , Chihara Dai , Castillo Luis Malpica , Nair Ranjit , Jain Preetesh , Neelapu Sattva S. , Rodriguez Maria A. , Strati Paolo , Nastoupil Loretta J. , Gunther Jillian R. , Dabaja Bouthaina S. , Pinnix Chelsea C. , Wu Susan Y. , Fang Penny Q. 
Published: September 2024

BackgroundDiffuse large B-cell lymphoma (DLBCL) involving the gastrointestinal (GI) organs is rare, and real-world outcomes after combined modality therapy (CMT) with systemic therapy (ST) and radiotherapy (RT) are not well-characterized, particularly in the contemporary era. We characterized outcomes in a large cohort of GI-DLBCL patients treated with ST alone or CMT.MethodsPatients with GI-DLBCL treated at a single institution were retrospectively reviewed. Kaplan-Meier and Cox regression models estimated survival. Multivariable analyses were conducted using the Cox proportional hazards model.ResultsOf 204 patients, gastric involvement was most common (63%). Most presented with early-stage disease (61%). All patients received ST and 65 patients (32%) received RT, 88% as part of first-line CMT. Median dose was 36 Gy (IQR 30.6–39.6) in 18 fractions (IQR 17–22). Median follow-up was 46 months. Five-year overall survival (OS) and progression-free survival (PFS) was 88% and 84%, respectively; complete response (CR) rate was 82%. Improved OS associated with low IPI (p=0.001), fewer chemotherapy lines (p&lt;0.001), early stage (p&lt;0.006), and CR (p&lt;0.001). Survival did not differ by RT receipt (p&gt;0.25). Only early stage and CR correlated with improved OS on multivariable analysis. Stomach-directed RT vs. RT to other sites correlated with improved PFS and OS (p&lt;0.04). Patients with early stage DLBCL treated with CMT in the post-rituximab era had equivalent OS vs. ST alone, even with fewer chemotherapy cycles (p&lt;0.02; median of 4 with RT vs. 6 cycles without). Fifty patients had bulky disease (≥7.5 cm), of whom 18 (36%) had early stage disease. Among patients with bulky disease, 5 (10%) developed relapse at the initial site of disease bulk. Four of the 5 patients did not receive consolidative radiation. Among these 4 patients, 3 relapsed only in their initial site of bulky disease. Of 191 patients with luminal GI-DLBCL, n=4 (2.1%) developed perforation; only one received RT. Acute Grade 3 toxicities were reported in 41.2% of patients, and 12 (5.8%) patients had late Grade 3 toxicities, 99% attributed to chemotherapy.ConclusionGI-DLBCL patients have favorable outcomes after CMT with minimal late toxicity. CMT may be offered with abridged systemic regimens with equivalent outcomes. Stomach directed-RT may mitigate relapse risk associated with incomplete disease response or bulky disease.
Post-conization pathological upgrading and outcomes of 466 patients with low-grade cervical intraepithelial neoplasia
Authors: Guo Yulin , Cai Hongning , Peng Qiuzi , Wang Ying , Li Lu , Zou Miao , Guo Jinyue , Wang Chaonan , Wu Xufeng , Ma Quanfu 
Published: September 2024

IntroductionThe management of patients with low-grade cervical intraepithelial neoplasia (CIN1) remains controversial. We analyzed the pathological upgrading rates of patients with CIN1 undergoing conization, identifying influencing factors, and compared their outcomes to those of patients with CIN1 receiving follow-up only.MethodsThis retrospective study included 466 patients with CIN1 confirmed by histopathology and treated with conization. Postoperative pathological upgrading was determined and its influencing factors were identified. We also analyzed post-conization outcomes, examining the rate of persistent/recurrent CIN1 and its influencing factors, and comparing these results to those of patients receiving follow-up only.ResultsThe pathological upgrading rate of patients with CIN1 after conization was 21.03% (98/466), and the influencing factors were preoperative high-risk human papillomavirus (HR-HPV) infection and cytological results. The upgrading rates of HR-HPV positive and negative patients were 22.05% and 0.00%, respectively (χ2 = 5.03, P=0.03). The upgrading rate of patients with cytological results negative for intraepithelial lesion malignancy was 10.94%, while the upgrading rates of atypical squamous cells, cannot exclude high-grade lesion(ASC-H) and high-grade squamous intraepithelial lesion(HSIL) groups were 47.37% and 52.94%, respectively (χ2 = 22.7, P=0.03). Persistent/recurrent CIN1 rates in the conization group were 21.24%, 15.97%, and 6.67% at 6, 12, and 24 months, respectively, significantly lower than those in the follow-up only group. The CIN2 progression rate in the conization group (0.26%) during the 24-month follow-up period was also significantly lower than that in the follow-up only group (15.15%; χ2 = 51.68, P&lt;0.01). The only factor influencing postoperative persistent/recurrent CIN1 was preoperative HR-HPV status. No patients who were HR-HPV negative preoperatively exhibited persistent/recurrent CIN1, compared with 25.55% of those who were HR-HPV positive preoperatively (χ2 = 4.40, P=0.04).DiscussionThe risk of progression to CIN2+ in the medium term is higher in patients with CIN1 receiving follow-up than in those undergoing conization. Doctors should refer to the guidelines but comprehensively consider age, fertility requirements, preoperative HR-HPV and cytological results, follow-up conditions, and other factors to select the most appropriate treatment strategy for patients with CIN1.
The potential of retinoic acid receptors as prognostic biomarkers and therapeutic targets in gastric cancer
Authors: Garattini Silvio Ken , Basile Debora , De Re Valli’ , Brisotto Giulia , Miolo Gianmaria , Canzonieri Vincenzo , Aprile Giuseppe , Corvaja Carla , Buriolla Silvia , Garattini Enrico , Puglisi Fabio 
Published: September 2024

BackgroundGastric cancer is a heterogeneous collection of tumors characterized by low survival rates. All-trans retinoic acid (retinoic-acid) is a clinically useful therapeutic agent belonging to the chemical family of retinoids, which consists of both natural and synthetic derivatives of vitamin-A. Retinoids are essential components of the normal diet and they regulate different physiological processes. From a therapeutic point of view, retinoic-acid is the first example of clinically useful differentiating agent. Indeed, the differentiating properties of this compound have promoted the use of retinoic-acid as a standard of care in Acute-Promyelocytic-Leukemia, a rare form of acute myeloid leukemia. In this study, we determine the RNA expression of the six isoforms of Retinoic-Acid-Receptors (RARα/RARβ/RARγ/RXRα/RXRβ/RXRγ) in view of their potential use as gastric cancer progression markers and/or therapeutic targets. In addition, we evaluate associations between the expression of these receptors and a simplified molecular classification of stomach tumors as well as the clinical characteristics of the cohort of patients analyzed. Finally, we define the prognostic value of the various Retinoic-Acid-Receptors in gastric cancer.MethodsIn this single institution and retrospective RAR-GASTRIC study, we consider 55 consecutive gastric cancer patients. We extract total RNA from the pathological specimens and we perform a NanoString Assay using a customized panel of genes. This allows us to determine the expression levels of the RAR and RXR mRNAs as well as other transcripts of interest.ResultsOur data demonstrate ubiquitous expression of the RAR and RXR mRNAs in gastric cancers. High levels of RARα, RARβ, RXRα and RXRβ show a significant association with stage IV tumors, “de novo” metastatic disease, microsatellite-stable-status, epithelial-to-mesenchymal-transition, as well as PIK3CA and TP53 expression. Finally, we observe a worse overall-survival in gastric cancer patients characterized by high RARα/RARβ/RARγ/RXRβ mRNA levels.ConclusionsIn gastric cancer, high expression levels of RARα/RARβ/RARγ/RXRβ transcripts are associated with poor clinical and molecular characteristics as well as with reduced overall-survival. Our data are consistent with the idea that RARα, RARβ, RARγ and RXRβ represent potential prognostic markers and therapeutic targets of gastric cancer.
Isoliquiritigenin ameliorates abnormal oligodendrocyte development and behavior disorders induced by white matter injury
Authors: Wu Dong , Zhou Wenjuan , Du Jingyi , Zhao Tiantian , Li Naigang , Peng Fan , Li Anna , Zhang Xinyue , Zhang Meihua , Hao Aijun 
Published: September 2024

BackgroundWhite matter injury is a predominant form of brain injury in preterm infants. However, effective drugs for its treatment are currently lacking. Previous studies have shown the neuroprotective effects of Isoliquiritigenin (ISL), but its impact on white matter injury in preterm infants remains poorly understood.AimsThis study aimed to investigate the protective effects of ISL against white matter injury caused by infection in preterm infants using a mouse model of lipopolysaccharide-induced white matter injury, integrating network pharmacology as well as in vivo and in vitro experiments.MethodsThis study explores the potential mechanisms of ISL on white matter injury by integrating network pharmacology. Core pathways and biological processes affected by ISL were verified through experiments, and motor coordination, anxiety-like, and depression-like behaviors of mice were evaluated using behavioral experiments. White matter injury was observed using hematoxylin-eosin staining, Luxol Fast Blue staining, and electron microscopy. The development of oligodendrocytes and the activation of microglia in mice were assessed by immunofluorescence. The expression of related proteins was detected by Western blot.ResultsWe constructed a drug-target network, including 336 targets associated with ISL treatment of white matter injury. The biological process of ISL treatment of white matter injury mainly involves microglial inflammation regulation and myelination. Our findings revealed that ISL reduced early nerve reflex barriers and white matter manifestations in mice, leading to decreased activation of microglia and release of proinflammatory cytokines. Additionally, ISL demonstrated the ability to mitigate impairment in oligodendrocyte development and myelination, ultimately improving behavior disorders in adult mice. Mechanistically, we observed that ISL downregulated HDAC3 expression, promoted histone acetylation, enhanced the expression of H3K27ac, and regulated oligodendrocyte pro-differentiation factors.ConclusionThese findings suggest that ISL can have beneficial effects on white matter injury in preterm infants by alleviating inflammation and promoting oligodendrocyte differentiation.
A comprehensive study of psychological well-being and traditional Chinese medicine constitutions among model workers in Beijing
Authors: Cao Hongli , Chen Xianyang , Song Yige , Li Shawn Xiang , Ma Hui , Zhang Guosheng , Gong Tianyu , Yu Hong , Liu Zijin 
Published: September 2024

BackgroundBody constitution is the foundation of Traditional Chinese Medicine (TCM), and model workers consist of a special group of workers within China. This study aims to research the relationship between the physical body constitutions based on TCM and the mental health of model workers.MethodsWe recruited 314 model workers from Beijing Rehabilitation Hospital to conduct the questionnaires such as SCL-90 and CCMQ to investigate if there is an association between mental health status and TCM body constitutions. We performed a Partial Least Squares Path Modeling (PLS path modeling).ResultsOur path model results revealed associations between different TCM constitution types and SCL scores, which serve as indicators of psychological well-being. Our research findings demonstrate a strong correlation between the Balanced constitution and elevated levels of psychological well-being, with a path coefficient of -0.503. In contrast, the other eight constitutional types exhibit path coefficients exceeding 0.3, indicating a tendency toward lower levels of psychological well-being. We also investigated the intricate connections between various TCM constitutional types and both mild and severe psychological well-being.ConclusionIn conclusion, the Balanced constitution continues to be closely associated with higher levels of psychological well-being, while the remaining eight body constitution types are consistently linked to lower levels of psychological well-being.
Analysis of the health status and its influencing factors of the low-income populations in Wuxi, China
Authors: Li Shiming , Jiang Ying , Wu Yue , Ji Yingying , Tian Lin , Yang Queping , Zhu Haohao 
Published: September 2024

ObjectiveTo understand the physical health condition and its influencing factors among the low-income population.MethodLow-income residents who visited or consulted at our Hospital during 2022 were selected for this study. Through telephone or face-to-face interviews, a self-made basic information questionnaire was used for data collection. The physical health level of the low-income population was analyzed, and a logistic regression model was applied to study its influencing factors.ResultsA total of 2,307 people were included in this study, of which 2,069 had various types of diseases, indicating a disease rate of 89.68%. Multivariate logistic regression analysis showed that age ≥ 60 years old (OR = 1.567, 95%CI: 1.122–2.188), poor mental health status (OR = 2.450, 95%CI: 1.203–3.678), smoking (OR = 1.752, 95%CI: 1.269–2.206), pulse pressure difference ≥ 60 (OR = 1.485, 95%CI: 1.164–1.787), and poor hearing (OR = 1.268, 95%CI: 1.026–1.324) were risk factors for disease, whereas being female (OR = 0.729, 95%CI: 0.540–0.984) was a protective factor for physical health.ConclusionAs a developing country with a large population, we should particularly focus on the physical health issues of the low-income population, take targeted measures for disease situations, and improve the quality of life of the low-income population.
Exploring the social context of insomnia: a thematic content analysis of the lived experiences of insomnia of Latinx women and men
Authors: Giorgio Cosenzo Luciana , Arias Darwin , Alcántara Carmela 
Published: September 2024

IntroductionAlthough social ties have detrimental and beneficial effects on sleep health, the social processes through which social ties affect sleep remain understudied, particularly among Latinx adults. These processes include social support, social stress, social control, personal control, and symbolic meaning. Importantly, some studies suggest there are gender differences in how social ties influence Latinx health. This study explores how Latinx women and men with insomnia describe the social processes through which social ties shape their lived experience of insomnia.MethodsQualitative data from six focus groups conducted in 2017 were analyzed using thematic content analysis. These focus groups were comprised of 35 Spanish- and English-speaking Latinx adults with insomnia. Participants were majority women (62.86%), had a mean age of 65.43 years (SD = 12.63), and an average insomnia severity score of 20.6 (SD = 3.44). Major and minor themes were deductively and inductively identified in the transcripts. These themes were compared between Latinx women and men. The methods and results of this study are reported using the Consolidated Criteria for Qualitative Research (COREQ).ResultsThe influence of social processes on the lived experience of insomnia centered on three major themes. The first theme identified social stress and social control as precipitating and perpetuating factors of insomnia. The second theme related to how social support, through receiving advice from others, shaped participants' coping behaviors. Lastly, participants described their insomnia symptoms as negatively affecting their social relationships through social conflict. Latina women attributed their insomnia to the symbolic meaning of their gender identities, while men did not.DiscussionThese findings suggest that among Latinx adults, it may be important for psychological treatments for insomnia to address the ways in which social processes contribute to insomnia symptoms. Additionally, Latina women's discussion of the influence of gender identities on their insomnia symptoms highlights the need to tailor psychological treatments for Latina women that acknowledge and address the challenges presented by traditional gender roles and expectations. Future studies should investigate the potential reciprocal relationship between social processes, mainly social stress and social control, and insomnia symptoms.