bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Divalent Cations on Outward Potassium Currents in Leech Retzius Nerve Cells
Authors: Jovanovic Zorica , Mihaljevic Olgica , Kostic Irena 
Published: November 2016

AbstractThe present study examines the effects of divalent metals, cadmium (Cd2+) and manganese (Mn2+), on the outward potassium currents of Retzius cells in the hirudinid leeches Haemopis sanguisuga using conventional two-microelectrode voltageclamp techniques. The outward potassium current is activated by depolarization and plays an important role in determining both the neuronal excitability and action potential duration. A strong inhibition of the fast current and a clear reduction in the late currents of the outward current with 1 mM Cd2+were obtained, which indicated that both components are sensitive to this metal. Complete blockage of the fast and partial reduction of the slow outward currents was observed after adding 1 mM Mn2+to the extracellular fluid. These data show that the outward K+current in leech Retzius nerve cells comprises at least two components: a voltage-dependent K+current and a Ca2+- activated K+current. These observations also indicate that Cd2+is more eff ective than Mn2+in blocking ion fl ow through these channels and that suppressing Ca2+-activated K+outward currents can prolong the action potential in nerve cells.
Anatomical Parameters of the Acetabulum in Heavy Vehicle Operators
Authors: Jeremic Dejan , Vulovic Maja , Macuzic Ivana Zivanovic , Ivosevic Anita , Grbovic Vesna , Sekulic Igor , Djordjevic Dusica 
Published: November 2016

AbstractIt has been suggested that long-term exposure by heavy vehicle operators to whole-body vibration (WBV) may be related to an increased risk of pathological changes in the anatomical parameters of the hip. The aim of this study was to explore the difference in anatomical parameters of acetabulum in drivers of heavy vehicles (experimental group; n=60) and subjects who have not been exposed to WBV (control group; n=60). The anteroposterior radiographic view of the hips was used to measure the following parameters: the vertical centre edge (VCE), the ‘horizontal toit externe’ angle (HTE), the neck shaft angle (NSA) and the acetabular depth (AD). Compared with the control group, the mean VCE angle values and AD were signifi cantly lower, while the average HTE and NSA values were signifi cantly higher in the experimental group. This study supports the hypothesis that exposure to whole-body vibration during operation of a vehicle causes an increased risk of acetabular dysplasia.
Correlations Between Clinical Parameters and Health-Related Quality of Life in Postmenopausal Osteoporotic Women
Authors: Grbovic Vesna , Skevin Aleksandra Jurisic , Ilic Katarina Parezanovic , Tomic Aleksandra Lucic , Nurkovic Jasmin , Jeremic Dejan , Djordjevic Dusica 
Published: November 2016

Abstract
The purpose of this study was to assess the correlation between health-related quality of life (HRQoL) and clinically relevant osteodensitometric and biochemical parameters in postmenopausal osteoporotic women. Bone mineral density (BMD) and T scores of the lumbar vertebrae and femoral neck were assessed in 100 osteoporotic women (56 without previous fractures and 44 with previous fractures) using dual x-ray absorptiometry. The Fracture Risk Assessment Tool (FRAX) index for major osteoporotic and hip fractures was calculated based on demographic data and hip BMD. Venous blood samples were taken from each subject for biochemical analysis (serum calcium, phosphorus, alkaline phosphatase and vitamin D levels). HRQoL was assessed using the QUALEFFO-41 questionnaire (domains: Health perception, Pain, and Physical, Social and Mental function). Basic participant characteristics (age, menopause length, body mass index, smoking habits, hereditary tendency towards fracture, fracture history) correlated with some of the QUALEFFO-41 domains, but the correlation coefficients were low (r<0.3), except in the case of the correlation between Pain and fracture history (r=0.638). Of the six variables included in the multiple regression model, fracture history was shown to be the most significant predictor with respect to the following three QUALEFFO-41 domains: Pain (b=20.511), Social function (b=2.548) and Health perception (b=3.185). Correlation analysis showed that after adjustment for basic characteristics, BMD and T score of the femoral neck and Pain (r=0.331 and r=0.449, respectively), Social function (r=0.422 and r=0.419) and Health perception (r=0.434 for T score of the femoral neck) exhibited the strongest correlations. Vitamin D was negatively correlated with Mental function, while the other biochemical parameters exhibited variable correlations with the QUALEFFO- 41 domains (r≈0.2-0.5). Our study confirmed the previously established relationship between BMD of the femoral neck and HRQoL in patients with osteoporosis and demonstrated correlations between various blood bone metabolism parameters and HRQoL that have not been previously investigated.
Higher Serum Uric Acid Levels in Multiple Sclerosis Patients After Longterm Interferon Beta Treatment
Authors: Toncev Gordana , Vesic Katarina , Aleksic Dejan , Lazarevic Snežana 
Published: November 2016

Abstract
Interferon beta is a safe and efficacious treatment for relapsing multiple sclerosis (MS). However, there is some evidence that uric acid, a scavenger of peroxynitrite, is involved in MS pathology and that increasing serum uric acid levels might have beneficial therapeutic effects. The aim of this study is to investigate serum uric acid levels in MS patients before and after long-term interferon beta treatment. Blood samples from 101 MS patients (53 receiving interferon beta 1a treatment and 48 receiving interferon beta 1b treatment; 28 male and 73 female; mean age at treatment onset 32,4±7,3 years; mean duration of disease at treatment onset 5,1±3,2 years; mean EDSS 2±1,3) before and after interferon beta treatment (mean treatment duration 3±2 years) were analysed. Serum uric acid levels were measured using a quantitative enzymatic assay (Elitech Diagnostic, Sees, France). MS patients had significantly increased serum uric acid levels after treatment compared with those at the beginning of treatment (272,31±78,21 μmol/l vs. 210,17±53,65 μmol/l; p=0,019, Wilcoxon Mann-Whitney U-test). We did not find significant differences in serum uric acid levels between the interferon beta 1a and interferon beta 1b groups (p=0.98). These results indicate that one of the beneficial effects of interferon beta in MS might be based on the elevation of serum uric acid levels as a natural scavenger of peroxynitrite.
Usage of Intramammary Antimicrobial Veterinary Medicinal Products in The Republic of Serbia from 2011 to 2014
Authors: Andjelkovic Jelena , Radonjic Vesela 
Published: November 2016

Abstract
Prudent use of antimicrobial medicine is an imperative in both human and veterinary medicine today. Antibiotic usage in humans and animals has increased over the years, consequently giving rise to antimicrobial resistance in pathogenic microorganisms. Mastitis is one of the most common conditions in bovine species, and intramammary antibacterial medicinal products are used in animal husbandry for mastitis treatment and prophylaxis.
This paper presents the quantities of intramammary antibiotics sold in the Republic of Serbia from 2011 to 2014 based on data reported to the Medicines and Medical Devices Agency of Serbia by Marketing Authorization Holders. Furthermore, we assessed the number of treated cows and compared those results with the reported total number of cows in the same time period, providing information on animal exposure to particular antibiotics.
In 2011 and 2012, beta-lactams were the most commonly used antimicrobials, while beta-lactams in combination with other substances were the most commonly used antibiotics in 2014, with a total of 80,927 treated animals. From 2011 to 2014, 15-25% of cows were treated with intramammary antimicrobials.
Bearing in mind the growing importance of antibiotic resistance in humans and animals, these results reveal the scope of the potential human exposure to antibiotics via consumption of the milk of treated cows.
The Influence of Different Types of Physical Activity on The Redox Status of Scuba Divers
Authors: Radojevic-Popovic Radmila , Nikolic Tamara , Stojic Isidora , Jeremic Jovana , Srejovic Ivan , Pesic Goran , Jakovljevic Vladimir 
Published: November 2016

Abstract
The effect of scuba diving on ROS production and oxidative stress compared to that of other recreational activities is still poorly understood. The aim of this study was to assess the influence of different types of physical activity on the redox status of scuba divers by testing the pro- and anti-oxidative parameters immediately before and after different types of physical load. The prevalence study included 10 professional police divers. All examinees were male, 32 ± 5.1 years of age, well-trained, and with a minimum of five to a maximum of 20 years of diving experience. The study was divided into three experimental protocols: 1) an exercise test (at atmospheric pressure), 2) an at sea dive (30 meters for 30 minutes), and 3) a dive into river current (10 meters for 30 minutes). Immediately before and after the load test of the divers at atmospheric pressure and immediately before and after the dive, blood samples were taken to determine the values of the following pro-oxidant markers: O2
−, H2O2, NO2
− and TBARS, as well as antioxidant enzymes (SOD and CAT). A comparison of the results before and after physical activity for all three protocols revealed a significant increase in values for NO2
−, O2
−, H2O2 and CAT after physical activity. It can be concluded that the values of all oxidative stress markers depend on the season of the year in which the research is conducted or on the frequency of dives and degree of physical exertion during this period of the year.
Synthesis, Characterization, and Cytotoxicity of Binuclear Cooper(II)-Complexes with some S-Alkenyl Derivatives of Thiosalicyclic Acid
Authors: Tomovic Dusan Lj. , Bukonjic Andriana M. , Kocovic Aleksandar , Nikolic Milos V. , Mijajlovic Marina Z. , Jevtic Verica V. , Ratkovic Zoran R. , Arsenijevic Aleksandar N. , Milovanovic Jelena Z. , Stojanovic Bojana , Trifunovic Srecko R. , Radic Gordana P. 
Published: November 2016

AbstractNew complexes of copper(II) with S-alkenyl derivatives of thiosalicylic acid (alkenyl = propenyl-(L1), isobutenyl-(L2)) have been synthesized and characterized by microanalysis, infrared spectra, magnetic measurements, and by NMR spectra. The cytotoxic activity of two newly synthesized precursor S-alkenyl derivatives of thiosalicylic acid were tested using an MTT colorimetric technique on HCT-116 human colon carcinoma cells. The cytotoxic effect of the copper(II)- complexes were higher compared to the cytotoxicity of the corresponding ligand (for concentrations from 31.25 to 250 μM). Copper(II)-complexes showed a slightly lower cytotoxicity compared to cisplatin. Complexes of copper(II) with S-alkenyl derivatives of thiosalicylic acid (at concentrations from 250 to 1000 μM) had a cytotoxic effect on HCT-116 cells compared to cisplatin.
Impact of the Crosslinker’s Molecular Structure on the Aggregation of Gold Nanoparticles
Authors: Deffner Michael , Schulz Florian , Lange Holger 
Published: November 2016

AbstractWe studied the aggregation of AuNP induced by small aromatic molecules under different conditions. In water, the aggregation was found to be difficult to control. Phase transfer of the particles into toluene by using oleylamine as a ligand allows for a more controlled and reliable synthesis. Using nonane-1,9-dithiol as a control, our experiments demonstrate that the molecular structure of the linker has a decisive influence on the aggregation. Aromatic dithiols yielded spherical aggregates in the range of 100 nm, whereas the aliphatic linker produced large aggregates in the µm range. The length of the aromatic linker (2 vs. 3 phenylene units) strongly affected aggregation kinetics and the structure of the produced aggregates. With UV/Vis and DLS based experiments it was possible to distinguish the process of ligand layer formation and aggregation. Our results will help to develop syntheses of defined spherical aggregates and possibly more complex structures.
Targeting the KRAS Pathway in Non-Small Cell Lung Cancer
Authors: Tomasini Pascale , Walia Preet , Labbe Catherine , Jao Kevin , Leighl Natasha B. 
Published: November 2016

Abstract

Lung cancer remains the leading cause of cancer-related deaths worldwide. However, significant progress has been made individualizing therapy based on molecular aberrations (e.g., EGFR, ALK) and pathologic subtype. KRAS is one of the most frequently mutated genes in non-small cell lung cancer (NSCLC), found in approximately 30% of lung adenocarcinomas, and is thus an appealing target for new therapies. Although no targeted therapy has yet been approved for the treatment of KRAS-mutant NSCLC, there are multiple potential therapeutic approaches. These may include direct inhibition of KRAS protein, inhibition of KRAS regulators, alteration of KRAS membrane localization, and inhibition of effector molecules downstream of mutant KRAS. This article provides an overview of the KRAS pathway in lung cancer and related therapeutic strategies under investigation.
Sunitinib: Ten Years of Successful Clinical Use and Study in Advanced Renal Cell Carcinoma
Authors: Motzer Robert J. , Escudier Bernard , Gannon Andrew , Figlin Robert A. 
Published: November 2016

Abstract
The oral multikinase inhibitor sunitinib malate was approved by the U.S. Food and Drug Administration in January 2006 for use in patients with advanced renal cell carcinoma (RCC). Since then, it has been approved globally for this indication and for patients with imatinib-resistant or -intolerant gastrointestinal stromal tumors and advanced pancreatic neuroendocrine tumors. As we mark the 10-year anniversary of the beginning of the era of targeted therapy, and specifically the approval of sunitinib, it is worthwhile to highlight the progress that has been made in advanced RCC as it relates to the study of sunitinib. We present the key trials and data for sunitinib that established it as a reference standard of care for first-line advanced RCC therapy and, along with other targeted agents, significantly altered the treatment landscape in RCC. Moreover, we discuss the research with sunitinib that has sought to refine its role via patient selection and prognostic markers, improve dosing and adverse event management, and identify predictive efficacy biomarkers, plus the extent to which this research has contributed to the overall understanding and management of RCC. We also explore the key learnings regarding study design and data interpretation from the sunitinib studies and how these findings and the sunitinib development program, in general, can be a model for successful development of other agents. Finally, ongoing research into the continued and future role of sunitinib in RCC management is discussed.
Compreensão (con)textual em mídias sociais digitais
Authors: Barros Demétrio Alana Kercia , Pereira Alves Luiz Eleildo , Araújo Costa Maria Helenice 
Published: November 2016

Neste artigo, abordamos a questão da leitura sob o ponto de vista da complexidade. Considerando com Beaugrande (1997) que o texto é um evento comunicativo cuja configuração envolve elementos de diferentes naturezas, incluindo os participantes da interação, entendemos que as abordagens tradicionais da leitura, mesmo as que se voltam para a interação, não amparam satisfatoriamente a compreensão do fenômeno. Com o intuito analisar de que modo aspectos que caracterizam a leitura como atividade complexa se manifestam na compreensão (con)textual em mídias sociais digitais, apoiamo-nos nos trabalhos de Pellanda (2005) e de Franco (2011) e tomamos por base a teoria autopoiética de Maturana e Varela (1995), bem como as noções de emergência e incorporação de Hanks (2008). Por meio da análise de dois exemplos de interação veiculados em ambiente virtual, observamos que a leitura desencadeia a autopoiese dos leitores enquanto sistemas vivos.
Palavras-chave: Linguagem. Complexidade. Autopoiese. Leitura. Emergência. Incorporação.
Study of oxidative stress in patients of psoriasis
Authors: Kaur Manpreet , Sharma Saurabh , Kukreja Sahiba , Kaur Jasleen , Bassi Roopam 
Published: November 2016

<p class="abstract"><strong>Background:</strong> Psoriasis is a well-recognized chronic inflammatory papulosquamous disorder characterized by the formation of salmon colored papules and plaques with silvery white scales. The aim of this study was to do a comparative evaluation of the parameters of oxidative stress, namely glutathione peroxidase, malondialdehyde and superoxide dismutase in patients of psoriasis with normal healthy subjects</p><p class="abstract"><strong>Methods:</strong> 60 subjects in the age group of 30-70 years were included in the study; Group A consisted of 30 newly diagnosed psoriasis patients presenting to the Dermatology OPD and Group B consisted of 30 normal healthy individuals. Thorough history taking and clinical examination were done. The punch biopsy was performed for histopathological examination and markers of oxidative stress were measured in each case and these values were then compared to healthy controls and statistical analysis was performed.<strong></strong></p><p class="abstract"><strong>Results:</strong> On comparison of the two groups, the levels of anti-oxidants superoxide dismutase and glutathione peroxidase were found to be decreased in psoriasis patients (168.46±51.89 U/ml and 4121.63±1812.53 U/ml respectively) as compared to controls (237±39.30 U/ml and 8435±1397.54 U/ml respectively) which was statistically highly significant (p &lt;0.001) and the level of pro-oxidant malondialdehyde was found to be increased in patients of psoriasis (0.42±0.13 nmol/ml) in contrast to controls (0.08±0.06 nmol/ml)which was also statistically highly significant (p &lt;0.001).</p><p><strong>Conclusions:</strong> Keeping in consideration the increased oxidative stress in the patients of psoriasis, the anti-oxidant drugs can form an important part of the therapeutic ladder of psoriasis. </p>
Moderate-dose Regular Lifelong Alcohol Intake Changes the Intestinal Flora, Protects against Aging, and Keeps Spatial Memory in the Senescence-accelerated Mouse Prone 8 (SAMP8) Model
Authors: Shimizu Chikako , Oki Yasuhiro , Mitani Yutaka , Tsuchiya Youichi , Nabeshima Toshitaka 
Published: November 2016

Purpose: Heavy and long-term alcohol consumption increase the risk of alcohol-related diseases. Epidemiological studies show moderate drinking reduces the risk of mortality, cardiovascular diseases, and brain infarction in the J-shaped or U-shaped curve effect. However, why moderate drinkers may be healthy and non-drinkers may be ill in diverse populations remains controversial. Herein, we examined the relationship between moderate/lifelong alcohol intake and aging, especially aging-related cognitive functions in senescence-accelerated mouse prone 8 (SAMP8) model. Methods: SAMP8 model (5-week-old, male, n = 36), a model of age-related cognitive deficit, were group-housed (n = 6/cage) and provided free access to water (water group, n = 18) or 1% ethanol (EtOH group, n = 18, intake started when mice were 9 weeks old). The object recognition test (ORT) and object location test (OLT) were used to evaluate cognitive functions. The intestinal flora at the age of 87 weeks was analyzed by terminal restriction fragment length polymorphism (T-RFLP). Results: The lifespan of the EtOH-group mice was about 4 weeks longer than that of the water-group mice. In the EtOH group, spatial recognition impairment, assessed by OLT, was observed later (age, 73 weeks) than that in the water group (age, 52 weeks). The spinal curvature and skin conditions progressed significantly slower in the EtOH group than in the water group. Moreover, diarrhea symptoms only appeared in the water group, at the age of 82 weeks. The T-RFLP analysis of the intestinal flora indicated higher Lactobacillales order and lower Clostridium cluster XI in the EtOH group than in the water group, although those were extremely high in some mice close to death in both groups. Water-group mice with diarrhea presented significantly higher Clostridium cluster XI than did those without diarrhea (P = 0.017). Conclusion: Moderate alcohol intake changes intestinal flora and positively affects aging of SAMP8 model.
EDITORIAL: Plágio e ineditismo dos artigos
Authors: Amoras Fernando Castro 
Published: November 2016

<p>EDITORIAL: Plágio e ineditismo dos artigos</p><p>A partir deste ano de 2016, a revista Estação Científica (UNIFAP) passou a adotar, como instrumento auxiliar da seleção dos artigos que serão publicados, a checagem de plágio nos conteúdos dos trabalhos que recebemos. Consta no site da revista a informação de que todos os trabalhos passarão por verificação de plágio, e desta forma alerta-se que sejam apresentados à Estação Científica (UNIFAP) apenas os trabalhos que tenham autenticidade de autoria. </p>
O aluno do PROEJA no município de Muriaé: Escola Municipal “Professora Odaléia Morais de Azevedo”
Authors: Moreira Raquel Veggi , Lirio Larissa Mendonça 
Published: November 2016

<p class="MsoNormal" style="margin-bottom: .0001pt; text-align: justify; line-height: 150%; mso-hyphenate: none;"><span style="font-size: 12pt; line-height: 150%; font-family: 'Times New Roman', serif;">O presente artigo teve objetivo principal traçar um perfil do aluno do </span><span style="font-size: 12pt; line-height: 150%; font-family: 'Times New Roman', serif;">Programa Nacional de Integração da Educação Profissional com a Educação Básica na modalidade de Educação de Jovens e Adultos </span><span style="font-size: 12pt; line-height: 150%; font-family: 'Times New Roman', serif;">da Escola Municipal </span><span style="font-size: 12pt; line-height: 150%; font-family: 'Times New Roman', serif;">“Professora Odaléia Morais de Azevedo”, no município de Muriaé-MG. Optou-se como procedimento metodológico a revisão de literatura de cunho exploratório, bem como, a coleta de dados, através da aplicação de questionário, e sua análise dos dados foi quantificada utilizando-se estatística simples, em amostra de 55 estudantes da </span><span style="font-size: 12pt; line-height: 150%; font-family: 'Times New Roman', serif;">EJA (Educação de Jovens e Adultos). Observou-se que a maioria dos alunos é do sexo feminino, entre 41 e 62 anos, e compreendem este espaço</span><span style="font-size: 12.0pt; line-height: 150%;"> como uma oportunidade de melhoria de vida. A qualidade do ensino para esse público precisa passar pela boa gestão e qualificação dos docentes, além da motivação e atratividade para os discentes, com a finalidade de alcançar cada vez mais, melhores resultados no processo ensino-aprendizagem, além disso é preciso que os professores considerem a diversidade desses alunos.</span></p>