bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic Differences in Glutamine Utilization Lead to Metabolic Vulnerabilities in Prostate Cancer
Authors: Zacharias Niki Marie , McCullough Christopher , Shanmugavelandy Sriram , Lee Jaehyuk , Lee Youngbok , Dutta Prasanta , McHenry James , Nguyen Linda , Norton William , Jones Lawrence W. , Bhattacharya Pratip K. 
Published: November 2017

AbstractThe new oncologic paradigm of precision medicine is focused on identifying metabolic, proteomic, transcriptomic and genomic variabilities in tumors that can be exploited to tailor treatments and improve patient outcomes. Metabolic changes are a hallmark of cancer, and inhibition of metabolic pathways is now a major strategy in medicinal chemistry for targeting cancers. However, non-invasive biomarkers to categorize metabolic subtypes are in short supply. The purpose of this study was to characterize the intracellular and extracellular metabolic profiles of four prostate cancer cell lines with varying degrees of aggressiveness. We observed metabolic differences between the aggressive prostate cancer cell line PC3 and the even more aggressive, metastatic subline PC3M assessed by hyperpolarized in vivo pyruvate studies, nuclear magnetic resonance spectroscopy, and carbon-13 feeding studies. On further examination of the differences between these two cell lines, we found increased glutamine utilization in the metastatic PC3M subline that led directly to sensitivity to glutaminase inhibitor CB-839. Our study supports the theory that metastatic progression increases glutamine utilization and the inhibition of glutaminolysis could have clinical implications.
Flavonoids from Pterogyne nitens Inhibit Hepatitis C Virus Entry
Authors: Shimizu Jacqueline Farinha , Lima Caroline Sprengel , Pereira Carina Machado , Bittar Cintia , Batista Mariana Nogueira , Nazaré Ana Carolina , Polaquini Carlos Roberto , Zothner Carsten , Harris Mark , Rahal Paula , Regasini Luis Octávio , Jardim Ana Carolina Gomes 
Published: November 2017

AbstractHepatitis C virus (HCV) is one of the leading causes of liver diseases and transplantation worldwide. The current available therapy for HCV infection is based on interferon-α, ribavirin and the new direct-acting antivirals (DAAs), such as NS3 protease and NS5B polymerase inhibitors. However, the high costs of drug design, severe side effects and HCV resistance presented by the existing treatments demonstrate the need for developing more efficient anti-HCV agents. This study aimed to evaluate the antiviral effects of sorbifolin (1) and pedalitin (2), two flavonoids from Pterogyne nitens on the HCV replication cycle. These compounds were investigated for their anti-HCV activities using genotype 2a JFH-1 subgenomic replicons and infectious virus systems. Flavonoids 1 and 2 inhibited virus entry up to 45.0% and 78.7% respectively at non-cytotoxic concentrations. The mechanism of the flavonoid 2 block to virus entry was demonstrated to be by both the direct action on virus particles and the interference on the host cells. Alternatively, the flavonoid 1 activity was restricted to its virucidal effect. Additionally, no inhibitory effects on HCV replication and release were observed by treating cells with these flavonoids. These data are the first description of 1 and 2 possessing in vitro anti-HCV activity.
First-trimester vaginal microbiome diversity: A potential indicator of preterm delivery risk
Authors: Haque Mohammed Monzoorul , Merchant Mitali , Kumar Pinna Nishal , Dutta Anirban , Mande Sharmila S. 
Published: November 2017

AbstractPreterm birth is a leading cause of global neonate mortality. Hospitalization costs associated with preterm deliveries present a huge economic burden. Existing physical/biochemical markers for predicting preterm birth risk are mostly suited for application at mid/late pregnancy stages, thereby leaving very short time (between diagnosis and delivery) for adopting appropriate intervention strategies. Recent studies indicating correlations between pre/full-term delivery and the composition of vaginal microbiota in pregnant women have opened new diagnostic possibilities. In this study, we performed a thorough meta-analysis of vaginal microbiome datasets to evaluate the utility of popular diversity and inequality measures for predicting, at an early stage, the risk of preterm delivery. Results indicate significant differences (in diversity measures) between ‘first-trimester’ vaginal microbiomes obtained from women with term and preterm outcomes, indicating the potential diagnostic utility of these measures. In this context, we introduce a novel diversity metric that has significantly better diagnostic ability as compared to established diversity measures. The metric enables ‘early’ and highly accurate prediction of preterm delivery outcomes, and can potentially be deployed in clinical settings for preterm birth risk-assessment. Our findings have potentially far reaching implications in the fight against neonatal deaths due to preterm birth.
TRADD mediates the tumor necrosis factor-induced apoptosis of L929 cells in the absence of RIP3
Authors: Chang Xixi , Wang Lili , Wang Zicheng , Wu Shuai , Zhu Xiaoming , Hu Shiping , Wang Yu , Yu Jiyun , Chen Guozhu 
Published: November 2017

AbstractReceptor-interacting protein kinase 3 (RIP3) is a critical initiator in mediating necroptosis induced by tumor necrosis factor alpha (TNFα) in L929 cells, so knockdown of RIP3 inhibits TNFα-induced L929 cell necroptosis. However, RIP3 knockdown was shown to switch TNFα-induced necroptosis to apoptosis in L929 cells in other studies. Therefore, whether RIP3 knockdown blocks the TNFα-induced death of L929 cells is controversial. In this study, TNFα activated caspase pathway and induced cell death in RIP3 knockdown L929 cells, and the RIP3-independent cell death had been blocked by Z-VAD-FMK (pan-caspase inhibitor) or caspase 8 knockdown, demonstrating that RIP3 knockdown switched TNFα-induced necroptosis to caspase-dependent apoptosis. Although both TNF receptor type 1-associated death domain protein (TRADD) and RIP1 have been reported to mediate TNFα-induced apoptosis, the knockdown of TRADD, but not RIP1, suppressed TNFα-induced activation of the caspase pathway and subsequent apoptosis in RIP3 knockdown L929 cells. In addition, TRADD bound and activated caspase 8 during the RIP3-independent apoptosis process, indicating that TRADD initiates RIP3-independent apoptosis by activating the caspase pathway. Collectively, we identified the target and mechanism underlying RIP3-independent apoptosis and elucidated the coordinated roles of RIP3 and TRADD in mediating the programmed cell death of L929 cells following TNFα stimulation.
Active Sites of M(IV)-incorporated Zeolites (M = Sn, Ti, Ge, Zr)
Authors: Yang Gang , Zhou Lijun 
Published: November 2017

AbstractM(IV)-incorporated zeolites have recently aroused wide interest due to outstanding catalytic effects while their active sites remain largely elusive. Here periodic density functional theory calculations are conducted finding that active sites are determined jointly by identity of M(IV) ions, topology of zeolites, type of framework species and choice of T sites. All M2(IV) active sites in BEA zeolites are penta-coordinated with chemisorption of one water while subsequent water molecules that form only H-bonds promote chemisorption of the first water, especially the second water possessing comparable or even higher adsorption strengths as the first water; Ti(IV) and Ge(IV) active sites at the intersection remain penta-coordinated and Sn(IV) and Zr(IV) active sites prefer to hexa-coordination although potentially expanded to hepta-coordination. Different from other zeolites, Ti(IV) active sites in FER zeolites are hexa-coordinated as Sn(IV) active sites, due to the promoting effect of the first water. Lewis acidic defects expand Ti(IV) active sites to hexa-coordination while inhibit the formation of hepta-coordinated Sn(IV) species. Two forms of Brϕnsted acidic defects exist for Sn(IV) sites instead of only one for Ti(IV) sites, and all M(IV) Brϕnsted acidic defects, regardless of different acidic forms and M(IV) ions, can chemisorb only one water.
Giant Anisotropic Magnetocaloric Effect in Double-perovskite Gd2CoMnO6 Single Crystals
Authors: Moon J. Y. , Kim M. K. , Choi Y. J. , Lee N. 
Published: November 2017

AbstractThe magnetocaloric effect (MCE) is described by the change in temperature of a material by magnetic field variation and is a crucial subject in magnetism; it is motivated by the desire to enhance energy-efficient magnetic refrigeration for clean technology. Despite the recent discovery of the giant cryogenic MCE in double perovskites, the role of magnetic anisotropy has not yet been clearly discussed, because of the averaging effect of polycrystalline samples. Here, we investigated the anisotropic MCE in the single-crystal double perovskite Gd2CoMnO6. In addition to the ferromagnetic order of the Co2+ and Mn4+ moments, the large Gd3+ moments align below TGd = 21 K, exhibiting an isotropic nature. Because of the intricate temperature development of magnetically hysteretic behaviour and metamagnetism, the change in magnetic entropy along the c-axis appears to be relatively small. On the contrary, the smaller but almost reversible magnetization perpendicular to the c-axis leads to a large MCE with a maximum entropy change of 25.4 J/kg·K. The anisotropic MCE generates a giant rotational MCE, estimated as 16.6 J/kg·K. Our results demonstrate the importance of magnetic anisotropy for understanding the MCE and reveal essential clues for exploring suitable magnetic refrigerant compounds aiming at magnetic functional applications.
Randomness-induced quantum spin liquid on honeycomb lattice
Authors: Yamaguchi Hironori , Okada Masataka , Kono Yohei , Kittaka Shunichiro , Sakakibara Toshiro , Okabe Toshiki , Iwasaki Yoshiki , Hosokoshi Yuko 
Published: November 2017

AbstractQuantum entanglement in magnetic materials is expected to yield a quantum spin liquid (QSL), in which strong quantum fluctuations prevent magnetic ordering even at zero temperature. This topic has been one of the primary focuses of condensed-matter science since Anderson first proposed the resonating valence bond state in a certain spin-1/2 frustrated magnet in 1973. Since then, several candidate materials featuring frustration, such as triangular and kagome lattices, have been reported to exhibit liquid-like behavior. However, the mechanisms that stabilize the liquid-like states have remained elusive. Here, we present a QSL state in a spin-1/2 honeycomb lattice with randomness in the exchange interaction. That is, we successfully introduce randomness into the organic radial-based complex and realize a random-singlet (RS) state (or valence bond glass). All magnetic and thermodynamic experimental results indicate the liquid-like behaviors, which are consistent with those expected in the RS state. Our results suggest that the randomness or inhomogeneity in the actual systems stabilize the RS state and yield liquid-like behavior.
Evidence for unconscious regulation of performance in fatigue
Authors: Ishii Akira , Tanaka Masaaki , Yoshikawa Takahiro , Watanabe Yasuyoshi 
Published: November 2017

AbstractSince fatigue is prevalent in modern societies, it is necessary to clarify the neural mechanisms of fatigue. The regulation of performance through fatigue sensation is one of the mechanisms that decreases performance in fatigue. However, it is unknown whether subjective feeling of fatigue is necessary for the regulation of performance. Here, we examined whether decreased performance occurs without increased fatigue sensation through the experiment which was designed to test if fatigue can be learned unconsciously. Healthy male volunteers performed a fatigue-inducing hand-grip task for 10 min while viewing a target image presented without awareness. On the next day, they viewed a control and the target images presented with awareness and the neural activity caused by viewing the images was measured using magnetoencephalography. Results showed the level of fatigue sensation was not altered but grip-strength was decreased by viewing the target image on the second day. The level of beta band power in Brodmann’s area 31 was increased by viewing the target image and this increase was negatively associated with the decrease of grip-strength caused in the hand-grip task. These findings demonstrated that fatigue can be learned unconsciously and that there is a mechanism to decrease performance without fatigue sensation.
Increased light penetration due to ultrasound-induced air bubbles in optical scattering media
Authors: Kim Haemin , Chang Jin Ho 
Published: November 2017

AbstractLight is an attractive tool for high spatial- and contrast-resolution imaging, highly sensitive molecular imaging, and target-selective therapy, and it does not exhibit the risks associated with ionizing radiation. The main limitation of using light in clinical applications is its superficial imaging and therapeutic depth caused by high optical scattering in biological media. Here, we demonstrate that the scattering and thus defocusing of the incident light can be alleviated when simultaneously delivered ultrasound generates air bubbles in the pathway of the incident light, thus increasing the light penetration. The bubbles are temporally induced by ultrasound with an intensity that is sufficiently low to avoid tissue damage and act as a Mie scattering medium in which light is scattered predominantly in the forward direction. The change in the optical scattering property caused by the ultrasound is undone after cessation of the insonification. From the results, it is expected that this proposed method will open a new route for overcoming the limitations of current optical imaging and therapeutic techniques.
Equilibrium folding dynamics of meACP in water, heavy water, and low concentration of urea
Authors: Zhou Yang , Yang Daiwen 
Published: November 2017

AbstractMany proteins fold in apparent two-state behavior, as partially folded intermediates only transiently accumulate and easily escape detection. Besides a native form and a mainly unfolded form, we captured a partially unfolded form of an acyl carrier protein from Micromonospora echinospora (meACP) in the folding/unfolding equilibrium using chemical exchange saturation transfer NMR experiments. The C-terminal region of the partially unfolded form is mainly folded and the N-terminal is unfolded. Furthermore, to understand how the folding process of meACP is influenced by solvent environments, we compared the folding dynamics of meACP in D2O, H2O and low concentration of urea. As the environment becomes more denaturing from D2O to H2O and then to urea, the unfolded state becomes increasingly populated, and the folding rate decreases. Adding a small amount of urea, which does not change solvent viscosity, has little effects on the unfolding rates, while changing H2O to D2O reduces the unfolding rates possibly due to the increase of solvent viscosity. The quantified solvent effects on the protein folding Gibbs energy and activation energy suggest that the transition state of folding may have a similar structure to the native state of the protein.
Ilizarov external fixation versus plate internal fixation in the treatment of end-stage ankle arthritis: decision analysis of clinical parameters
Authors: Li Jun , Li Bohua , Zhang Zhengdong , Wang Shanxi , Liu Lei 
Published: November 2017

AbstractThe purpose of this study was to evaluate the effect of Ilizarov external fixation (IEF) and plate internal fixation (PIF) in the treatment of end-stage ankle arthritis on pain relieving and function improvement. The study cohort consisted of 59 patients with end-stage ankle arthritis underwent ankle arthrodesis with IEF or PIF between June 2011 and June 2015. Standard radiographs and computed tomography (CT) scans were obtained before surgery and during the follow-up. Functional assessments were performed using Foot and Ankle pain score of American Orthopedics Foot and Ankle Society (AOFAS) and Visual Analogue Scale (VAS). The average AOFAS scores in both IEF group and PIF groups increased significantly after operation, from 45.5 ± 6.3 to 84.8 ± 4.9 and from 45.9 ± 6.6 to 86.6 ± 5.4, respectively. The average VAS scores in both groups decreased significantly after operation, from 8.4 ± 1.9 to 2.5 ± 0.6 and from 8.2 ± 1.5 to 2.3 ± 0.7, respectively. Nevertheless, there was no significant difference for preoperative or postoperative AOFAS and VAS scores between the two groups. The IEF would result in comparable postoperative functional recovery and pain relieving to PIF and may be an effective substitute to PIF in the treatment of end-stage ankle arthritis.
p300-mediated acetylation increased the protein stability of HIPK2 and enhanced its tumor suppressor function
Authors: Choi Jong-Ryoul , Lee Seo-Young , Shin Ki Soon , Choi Cheol Yong , Kang Shin Jung 
Published: November 2017

AbstractHomeodomain-interacting protein kinase 2 (HIPK2) is a nuclear serine/threonine kinase that functions in development and tumor suppression. One of the prominent features of this kinase is that it is tightly regulated by proteasomal degradation. In the present study, we present evidence suggesting that the protein stability of HIPK2 can be regulated by p300-mediated acetylation. p300 increased the protein level of HIPK2 via its acetyltransferase activity. p300 increased the acetylation of HIPK2 while decreased polyubiquitination and its proteasomal degradation. We also observed that DNA damage induced acetylation of HIPK2 along with an increase in the protein amount, which was inhibited by p300 RNAi. Importantly, p300 promoted p53 activation and the HIPK2-mediated suppression of cell proliferation, suggesting acetylation-induced HIPK2 stabilization contributed to the enhanced activation of HIPK2. Overexpression of p300 promoted the HIPK2-mediated suppression of tumor growth in mouse xenograft model as well. Taken together, our data suggest that p300-mediated acetylation of HIPK2 increases the protein stability of HIPK2 and enhances its tumor suppressor function.
Advanced glycation endproducts link inflammatory cues to upregulation of galectin-1 in diabetic retinopathy
Authors: Kanda Atsuhiro , Dong Yoko , Noda Kousuke , Saito Wataru , Ishida Susumu 
Published: November 2017

AbstractDiabetic retinopathy (DR) is an inflammatory and progressive vaso-occlusive disease resulting in angiogenesis. Galectin-1 is a hypoxia-induced angiogenic factor associated with cancer and proliferative DR. Here we reveal a significant upregulation of galectin-1 in eyes of DR patients along with progression of clinical stages beginning from the pre-ischemic, inflammatory stage with diabetic macular edema, but not in eyes with non-diabetic retinal vascular occlusions. As for its regulatory mechanism unrelated to hypoxia but selective to DR, in vitro galectin-1/LGALS1 expression was shown to increase after application to Müller glial cells with interleukin (IL)-1β, which was induced in monocyte-derived macrophages and microglial cells via toll-like receptor (TLR) 4 signaling stimulated by advanced glycation endproducts (AGE). In vivo inhibition of AGE generation with aminoguanidine, macrophage depletion with clodronate liposomes, and antibody-based blockade of Il-1β and Tlr4 attenuated diabetes-induced retinal Lgals1 expression in mice. Fibrovascular tissues from proliferative DR eyes were immunoreactive for AGE, TRL4 and IL-1β in macrophages, and IL-1β receptor-positive glial cells expressed galectin-1. Therefore, diabetes-induced retinal AGE accumulation was suggested to activate IL-1β-related inflammatory cues in macrophages followed by Müller cells, linking to galectin-1 upregulation in human DR with time. Our data highlight AGE-triggered inflammation as the DR-selective inducer of galectin-1.
Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette
Authors: Huo Ya-Yun , Li Gui-Fen , Qiu Yan-Hong , Li Wei-Min , Zhang Yong-Jiang 
Published: November 2017

AbstractPrunus necrotic ringspot virus (PNRSV) is one of the most devastating viruses to Prunus spp. In this study, we developed a diagnostic system RT-CPA-NATSC, wherein reverse transcription-cross-priming amplification (RT-CPA) is coupled with nucleic acid test strip cassette (NATSC), a vertical flow (VF) visualization, for PNRSV detection. The RT-CPA-NATSC assay targets the encoding gene of the PNRSV coat protein with a limit of detection of 72 copies per reaction and no cross-reaction with the known Prunus pathogenic viruses and viroids, demonstrating high sensitivity and specificity. The reaction is performed on 60 °C and can be completed less than 90 min with the prepared template RNA. Field sample test confirmed the reliability of RT-CPA-NATSC, indicating the potential application of this simple and rapid detection method in routine test of PNRSV.
Phosphate-Solubilizing Bacteria Nullify the Antagonistic Effect of Soil Calcification on Bioavailability of Phosphorus in Alkaline Soils
Authors: Adnan Muhammad , Shah Zahir , Fahad Shah , Arif Muhamamd , Alam Mukhtar , Khan Imtiaz Ali , Mian Ishaq Ahmad , Basir Abdul , Ullah Hidayat , Arshad Muhammad , Rahman Inayat-Ur , Saud Shah , Ihsan Muhammad Zahid , Jamal Yousaf , Amanullah  , Hammad Hafiz Mohkum , Nasim Wajid 
Published: November 2017

AbstractPhosphate-solubilizing bacteria (PSB) reduce the negative effects of soil calcification on soil phosphorus (P) nutrition. In this incubation study, we explored the ability of PSB (control and inoculated) to release P from different P sources [single super phosphate (SSP), rock phosphate (RP), poultry manure (PM) and farm yard manure (FYM)] with various soil lime contents (4.78, 10, 15 and 20%) in alkaline soil. PSB inoculation progressively enriched Olsen extractable P from all sources compared to the control over the course of 56 days; however, this increase was greater from organic sources (PM and FYM) than from mineral P sources (SSP and RP). Lime addition to the soil decreased bioavailable P, but this effect was largely neutralized by PSB inoculation. PSB were the most viable in soil inoculated with PSB and amended with organic sources, while lime addition decreased PSB survival. Our findings imply that PSB inoculation can counteract the antagonistic effect of soil calcification on bioavailable P when it is applied using both mineral and organic sources, although organic sources support this process more efficiently than do mineral P sources. Therefore, PSB inoculation combined with organic manure application is one of the best options for improving soil P nutrition.
(Re)walking stories: pilgrimage, pedagogy and peace.
Authors: Terreault S. 
Published: November 2017

Abstract

This chapter deals with the methodological and pedagogical journey taken by the author and her colleague as they develop and teach an undergraduate course in pilgrimage studies. It first examines the notion of 'pilgrimage', and considers the implications for engaged, transdisciplinary, performative pedagogy, including the use of narrative as a legitimate mode of enquiry. The chapter then describes the experience of researching and walking a pilgrimage (in and around Montreal, Canada) that was designed for the class.
Travel and/or pilgrimage - both sacred journeys: an atheist's attempt at inquiry and introspection.
Authors: Chadwick Co Sy Su Chadwick Co Sy Su 
Published: November 2017

Abstract

This chapter begins by recognizing the many modes of pilgrimage and then discusses how religious and non-religious pilgrimage may have been (incorrectly) pitted against each other, and ends by proposing the creation of synonymy: of pilgrimage and travel being one and the same. The chapter discusses travel and/or pilgrimage as a source of inquiry and introspection and presents questions on a wide range of related topics, from the abstract to the practical. The potential for peacemaking and interdisciplinary collaboration among the fields of pilgrimage studies, tourism, anthropology and theology is also briefly discussed.