bluuebunny/crossref_metadata_2025_split huggingface dataset:
Northern marine transportation corridors: Creation and analysis of northern marine traffic routes in Canadian waters
Authors: Chénier René , Abado Loretta , Sabourin Olivier , Tardif Laurent 
Published: September 2017

AbstractThe concept of the Northern Marine Transportation Corridors (NMTC) initiative was developed under the Government of Canada World‐Class Tanker Safety System Initiative (WCTSS). The NMTC is an interdepartmental Arctic initiative within the Department of Fisheries and Oceans Canada (DFO), through the Canadian Coast Guard (CCG) and the Canadian Hydrographic Service (CHS), in collaboration with Transport Canada (TC). The NMTC initiative was developed to strengthen the safety of marine navigation in the Arctic, and to offer an efficient planning guide for present and future Arctic investments. Transportation corridors identified through this initiative will provide the Government of Canada the framework needed to better prioritize and deliver on its programs and services, including: nautical charts and products; aids to navigation; icebreaking services; and marine safety regulations. The corridors were generated and analyzed with a Geographic Information System (GIS) using two main data sources: the Automated Identification System (AIS) and CHS's nautical charts and publications. The geographic extent of the NMTC is defined as the Northern Canada Vessel Traffic Service Zone (NORDREG Zone) and the Mackenzie River. With close to 4 million km2 of water in the Arctic and 162,000 km of coastline, surveying the Arctic to modern standards represents an enormous challenge to CHS. By adopting a corridor‐based approach, CHS and other government programs can prioritize their efforts on 12% of the Canadian Arctic waters. CHS currently has 32% of the NMTC adequately surveyed, with an additional 3% surveyed to modern standards.
Validity of historical volunteered geographic information: Evaluating citizen data for mapping historical geographic phenomena
Authors: Zhang Guiming , Zhu A‐Xing , Huang Zhi‐Pang , Ren Guopeng , Qin Cheng‐Zhi , Xiao Wen 
Published: September 2017

AbstractStudies on volunteered geographic information (VGI) have focused on examining its validity to reveal geographic phenomena in relatively recent periods. Empirical evaluation of the validity of VGI to reveal geographic phenomena in historical periods (e.g., decades ago) is lacking, although such evaluation is desirable for assessing the possibility of broadening the temporal scope of VGI applications. This article presents an evaluation of the validity of VGI to reveal historical geographic phenomena through a citizen data‐based habitat suitability mapping case study. Citizen data (i.e., sightings) of the black‐and‐white snub‐nosed monkey (Rhinopithecus bieti) were elicited from local residents through three‐dimensional (3D) geovisualization interviews in Yunnan, China. The validity of the elicited sightings to reveal the historical R. bieti distribution was evaluated through habitat suitability mapping using the citizen data in historical periods. The results of controlled experiments demonstrated that suitability maps predicted using the historical citizen data had a consistent spatial pattern (correlation above 0.60) that reflects the R. bieti distribution (Boyce index around 0.90) in areas free of significant environmental change across historical periods. This in turn suggests that citizen data have validity for mapping historical geographic phenomena. It provides supporting empirical evidence for potentially broadening the temporal scope of VGI applications.
Effect of distal ulnar ostectomy on carpal joint stability during weight bearing in the dog
Authors: Amsellem Pierre M. , Young Aisha N. , Muirhead Tammy L. , Pack LeeAnn , Moak Peter , Matthews Andrea R. , Marcellin‐Little Denis J. 
Published: September 2017

AbstractObjectiveTo assess the influence of a 50% distal ulnectomy on mediolateral carpal stability in the dog.Study designCanine cadaveric study.Sample populationSeven canine thoracic limbsMethodsThoracic limbs were placed in a jig to mimic weight bearing with a load representing 30% of body weight. Carpal extension angle was standardized at 190° ± 5°. Frontal plane carpal angles were measured with the limb loaded on craniocaudal radiographs before and after ulnectomy. Valgus and varus stress radiographs with the limb loaded were acquired before and after ulnectomy. The limbs were palpated and were subjectively graded for valgus or varus instability by 2 investigators before and after ulnectomy.ResultsMean (±SD) valgus angulation increased after ulnectomy (2.1° ± 1.7°;P = .017; CI95 = 0.5°‐3.7°) when the limb was loaded without valgus or varus stress applied. Mean valgus angulation increased after ulnectomy (2.7° ± 2.8°;P = .032; CI95 = −0.2°‐5.5°) when valgus stress was applied to the loaded limb. Varus angulation was unchanged after ulnectomy (0.6° ± 4.6°;P = .383; CI95 = −4.2°‐5.3°) when varus stress was applied to the loaded limb. Palpation detected increased valgus score after ulnectomy.ConclusionDistal ulnectomy with excision of the lateral styloid process induces a slight increase in valgus in canine cadaver carpi. The clinical consequences of that valgus on carpal function and health should be assessed in clinical patients.
Video‐assisted microwave ablation for the treatment of a metastatic lung lesion in a dog with appendicular osteosarcoma and hypertrophic osteopathy
Authors: Mazzaccari Kaitlyn , Boston Sarah E. , Toskich Beau B. , Bowles Kristina , Case J. Brad 
Published: September 2017

AbstractObjectiveTo describe video‐assisted microwave ablation (VAMA) for the treatment of a metastatic lung lesion secondary to right forelimb osteosarcoma in a dog.Study designCase report.AnimalsA 10‐year‐old female spayed mixed breed dog with a metastatic lung lesion secondary to appendicular osteosarcoma.MethodsAn osteosarcoma of the right distal scapula and proximal humerus that was suspected to be a radiation‐induced osteosarcoma was treated with limb amputation and carboplatin chemotherapy. The patient developed pulmonary metastatic lesions and hypertrophic osteopathy (HO). VAMA of a metastatic lesion in the right caudal lung lobe was performed 227 days after amputation. The procedure was performed without complication.ResultsFollow‐up information with the referring veterinarian 40 days after VAMA indicated that the patient was stable and that the clinical signs of HO had resolved. Thoracic radiographs taken by the referring veterinarian (RDVM) at monthly intervals showed that the previously treated metastatic lesion was stable. At 134 days from VAMA, the patient presented to the RDVM for lethargy and dyspnea and was transferred to an emergency clinic. The patient arrested and died 136 days from the VAMA procedure while hospitalized. A postmortem was not performed.ConclusionVAMA for pulmonary metastatic lesions is technically feasible and allows for the treatment of symptoms associated with HO and minimally invasive management of pulmonary metastases in the case reported.
Could recent increases in atmospheric CO2 have acted as a selection factor in Avena fatua populations? A case study of cultivated and wild oat competition
Authors: Ziska L H 
Published: September 2017

SummaryProjected increases in atmospheric carbon dioxide concentration [CO2] may lead to differential selection and competition between weeds and crops. Yet, the current level of atmospheric [CO2] already reflects a rapid rise (~25%) from mid‐20th century levels. To assess whether this increase could have already resulted in differential selection between weeds and crops, two temporally distinct populations of Avena fatua (wild oat) from the same geographic location, one from the 1960s (WOold) and one from 2014 (WOnew), were grown with and without a cultivated oat (CO) line, Clintland 64 (Avena sativa) at current and mid‐20th century [CO2] levels (ca 320 and 402 μmol mol−1 respectively). Monocultures of each WO population differed in their response to recent increases in atmospheric [CO2], with WOnew showing a significantly higher response for all vegetative parameters relative to WOold. Assessment of competitive outcomes indicated that at 315 μmol mol−1 cultivated oat was at a competitive advantage relative to either A. fatua population for leaf area and total above‐ground biomass. However, at current levels of CO2, an overall increase in WOnew competitive ability was observed relative to the cultivated oat line. Overall, these differences are consistent with, but not conclusive for, improved evolutionary fitness and increased early competitive ability of A. fatua relative to cultivated oat as a function of the recent increase in atmospheric [CO2]. While additional empirical studies are needed, these preliminary results indicate that weeds may be adapting rapidly to rising levels of atmospheric carbon dioxide.
Encouraging experience using multi‐transgenic xenografts in a pig‐to‐baboon cardiac xenotransplantation model
Authors: Chan Joshua L. , Singh Avneesh K. , Corcoran Philip C. , Thomas Marvin L. , Lewis Billeta GT , Ayares David L. , Vaught Todd , Horvath Keith A. , Mohiuddin Muhammad M. 
Published: September 2017

AbstractBackgroundInnovations in transgenic technology have facilitated improved xenograft survival. Additional gene expression appears to be necessary to overcome the remaining immune and biologic incompatibilities. We report for the first time the novel use of six‐gene modifications within a pig‐to‐baboon cardiac xenotransplantation model.MethodsBaboons (8‐15 kg) underwent heterotopic cardiac transplantation using xenografts obtained from genetically engineered pigs. Along with previously described modifications (GTKO, hCD46), additional expression of human transgenes for thromboregulation (endothelial protein C receptor, tissue factor pathway inhibitor, thrombomodulin), complement inhibition (decay accelerating factor), and cellular immune suppression (hCD39, hCD47) was used. Immunosuppression consisted of targeted T‐cell and B‐cell depletion and conventional anti‐rejection agents.ResultsHeterotopic cardiac transplantations were performed without complication. Flow cytometry and immunohistochemistry on donor biopsies confirmed transgenic phenotype. In contrast to the prior three‐gene generation, significant coagulopathy or consumptive thrombocytopenia has not been observed in the six‐gene cohort. As a result, these recipients have experienced decreased bleeding‐related complications. Pro‐inflammatory responses also appear to be mitigated based on cytokine analysis. Baboons survived the critical 30‐day post‐operative period when mortality has historically been highest, with no evidence of graft rejection.ConclusionsThe inclusion of additional human genes in genetically engineered pigs appears to confer superior xenograft outcomes. Introduction of these genes has not been associated with adverse outcomes. This multifactorial approach to genetic engineering furthers the prospect of long‐term cardiac xenograft survival and subsequent clinical application.
Hyperbaric oxygenation and 20‐hydroxyeicosatetreanoic acid inhibition reduce stroke volume in female diabetic Sprague–Dawley rats
Authors: Mišir Mihael , Renić Marija , Novak Sanja , Mihalj Martina , Ćosić Anita , Vesel Monika , Drenjančević Ines 
Published: September 2017

New Findings
What is the central question of this study?
Is there a beneficial effect and what are the mechanisms of acute and multiple hyperbaric oxygenation (HBO2) exposures on the outcome of cerebral tissue injury induced by a transient middle cerebral artery occlusion model in diabetic female rats? Are 20‐hydroxyeicosatetreanoic acid and epoxyeicosatrienoic acids involved?

What is the main finding and its importance?
Equal reduction of cortical and total infarct size in rats treated with HBO2 and HET0016 (20‐hydroxyeicosatetreanoic acid production inhibitor) and significant mRNA upregulation of epoxyeicosatrienoic acid‐producing enzymes (Cyp2J3 and Cyp2C11) in treated groups suggest that HBO2 and HET0016 are highly effective stroke treatments and that cytochrome P450 metabolites are involved in this therapeutic effect.

We evaluated the effects of acute and repetitive hyperbaric oxygenation (HBO2), 20‐hydroxyeicosatetreanoic acid (20‐HETE) inhibition by N‐hydroxy‐N′‐(4‐butyl‐2methylphenyl)‐formamidine (HET0016) and their combination on experimental stroke outcomes. Streptozotocin‐induced type 1 diabetic Sprague–Dawley female rats (n = 42; n = 7 per group), were subjected to 30 min of transient middle cerebral artery occlusion (t‐MCAO)–reperfusion and divided into the following groups: (1) control group, without treatment; and groups exposed to: (2) HBO2; (3) multiple HBO2 (HBO2 immediately and second exposure 12 h after t‐MCAO); (4) HET0016 pretreatment (1 mg kg−1, 3 days before t‐MCAO) combined with HBO2 after t‐MCAO; (5) HET0016 treatment (1 h before, during and for 6 h after t‐MCAO); and (6) HET0016 treatment followed by HBO2 after t‐MCAO. Messenger RNA expression of CYP2J3, CYP2C11, CYP4A1, endothelial nitric oxide synthase and epoxide hydrolase 2 was determined by real‐time qPCR. Cortical infarct size and total infarct size were equally and significantly reduced in HBO2‐ and HET0016‐treated rats. Combined treatment with HET0016 and HBO2 provided no significant additive effect compared with HET0016 treatment only. Messenger RNA of Cyp2J3 was significantly increased in all study groups, and mRNA of Cyp2C11 was significantly increased in the multiple HBO2 group and the HET0016 treatment followed by HBO2 group, compared with the control group. Expression of endothelial nitric oxide synthase was significantly increased after HBO2 treatments, and expression of epoxide hydrolase 2 was increased in all groups compared with the control group. In diabetic female Sprague–Dawley rats, HBO2 and HET0016 are highly effective stroke treatments, suggesting the involvement of cytochrome P450 metabolites and the NO pathway in this therapeutic effect.
Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method
Authors: Pawlinski Bartosz , Domino Malgorzata , Zabielski Romuald , Siewruk Katarzyna , Polanska‐Plachta Malgorzata , Gajewski Zdzislaw 
Published: September 2017

New Findings
What is the central question of this study?
The aim of present study was to record and analyse the myoelectrical activity in the female pig reproductive tract (uterus and oviduct) during early pregnancy.

What is the main finding and its importance?
Understanding the contractile activity of the uterus and oviducts is indispensable for understanding the physiological mechanisms as well as all irregularities associated with the period of conception and early pregnancy.

The aim of the present study was to record the myoelectrical activity of the reproductive tract in sows during the oestrous phase and early pregnancy via a telemetry recording system. In a total of eight non‐pregnant pigs, the bioelectrical activity was recorded through three silicone electrodes sutured on the oviduct (isthmus and ampulla) and the uterine horn. Blood samples were collected to monitor the concentrations of progesterone (P4) and luteinizing hormone (LH). The oestrous cycle was synchronized with equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG), and the animals were subjected to artificial insemination. Analysis of the EMG activity of the oviduct and uterus in the oestrous phase and in early stages of pregnancy suggests explicitly that telemetry could enable in vivo assessment of myoelectrical activity of parts of the reproductive system in sows. Off‐line analysis of the duration of EMG activity bursts in the uterus, isthmus and ampulla were significantly higher during early pregnancy (phases II and III) than in the oestrous phase. The EMG signals demonstrated low mean amplitudes of activity in the oviduct and uterus during early pregnancy (phases I–III). Significant differences between the root mean square signals were observed in the isthmus and ampulla both during oestrus and in early pregnancy (phase I; P < 0.01). During the oestrous phase, the P4 concentration was estimated at <1 ng ml−1, whereas the LH concentration was >4 ng ml−1. In contrast, during early pregnancy, the P4 and LH concentrations were estimated at >4 and <1 ng ml−1, respectively.
Draft Genome Sequence of Natronolimnobius baerhuensis CGMCC 1.3597
T
, an Aerobic Haloalkaliphilic Archaeon Isolated from a Soda Lake
Authors: Guo Xiaomeng , Liao Ziya , Yan Yanchun , Holtzapple Mark , Hu Qingping , Zhao Baisuo 
Published: September 2017

ABSTRACT

The haloalkaliphilic archaeon
Natronolimnobius baerhuensis
was isolated from a soda lake in Inner Mongolia (China), growing optimally at about 20% NaCl and pH 9.0. The draft genome consists of approximately 3.91 Mb and contains 3,810 predicted genes. Some genes that regulate intracellular osmotic stress and pH homeostasis were identified, providing insight into specific adaptations to this double-extreme environment.
Genome Sequences for Multiple
Clavibacter
Strains from Different Subspecies
Authors: Li Xiang (Sean) , Yuan Xiaoli (Kat) 
Published: September 2017

ABSTRACT

The Gram-positive genus
Clavibacter
harbors economically important plant pathogens infecting a variety of agricultural crops, such as potato, tomato, corn, barley, etc. Here, we report five new genome sequences, those of strains CFIA-Cs3N, CFIA-CsR14, LMG 3663
T
, LMG 7333
T
, and ATCC 33566
T
, from different subspecies of
Clavibacter michiganensis
. All these genomic data will be used for reclassification and niche-adapted feature comparisons.
Complete Genome Sequences of Lactobacillus curvatus KG6,
L. curvatus
MRS6, and Lactobacillus sakei FAM18311, Isolated from Fermented Meat Products
Authors: Jans Christoph , Lagler Sandra , Lacroix Christophe , Meile Leo , Stevens Marc J. A. 
Published: September 2017

ABSTRACT

The genomes of
Lactobacillus curvatus
KG6,
L. curvatus
MRS6, and
Lactobacillus sakei
FAM18311 were sequenced and assembled using PacBio single-molecule real-time (SMRT) technology. The strains were isolated from Swiss fermented meat products. Circular chromosomes were of 1.98 Mbp (KG6), 2.11 Mbp (MRS6), and 1.95 Mbp (FAM18311), with a G+C content of 41.3 to 42.0%.
Complete Genome Sequences of
emm111
Type Streptococcus pyogenes Strain GUR, with Antitumor Activity, and Its Derivative Strain GURSA1 with an Inactivated
emm
Gene
Authors: Suvorova Maria A. , Tsapieva Anna N. , Bak Emilie Glad , Chereshnev Valery A. , Kiseleva Ekaterina P. , Suvorov Alexander N. , Arumugam Manimozhiyan 
Published: September 2017

ABSTRACT

We present here the complete genome sequence of
Streptococcus pyogenes
type
emm111
strain GUR, a throat isolate from a scarlet fever patient, which has been used to treat cancer patients in the former Soviet Union. We also present the complete genome sequence of its derivative strain GURSA1 with an inactivated
emm
gene.
Draft Genome Sequences of Two Strains of a Newly Described Species, Sphingobacterium cellulitidis
Authors: Seemann Torsten , Bulach Dieter M. , Carter Glen , Albert M. John 
Published: September 2017

ABSTRACT

The draft genome sequences of two strains of a newly described species,
Sphingobacterium cellulitidis
, have been determined. The type strain originated from cellulitis of a toe of a patient and the other strain from the environment. The sequences will provide the reference genomes of the new
Sphingobacterium
species.
Complete Genome Sequence of Bos taurus Papillomavirus Type 1, Isolated in Morocco
Authors: Regnard Guy L. , Matiso Asanda , Mounir Latif , Embarki Tarik , Hitzeroth Inga I. , Rybicki Edward P. 
Published: September 2017

ABSTRACT

Bos taurus
papillomaviruses infect cattle, which has both animal health and economic consequences. This is the first report and sequence of
Bos taurus
papillomavirus type 1 isolated from warts in cattle in Morocco. The double-stranded DNA (dsDNA) genome was 7,945 bp in size, and eight open reading frames were identified.
Genome Sequence of the Homoacetogenic, Gram-Negative, Endospore-Forming Bacterium Sporomusa acidovorans DSM 3132
Authors: Humphreys Jonathan R. , Daniel Rolf , Poehlein Anja 
Published: September 2017

ABSTRACT

Sporomusa acidovorans
DSM 3132 is a strictly anaerobic, spore-forming and acetogenic bacterium, which was isolated from effluent of an alcohol distillation fermenter. The genome harbors genes involved in the Wood-Ljungdahl pathway for carbon fixation and several genes for glycerol metabolism. The genome (6.06 Mb) contains 4,506 predicted protein-encoding genes.
Genomic Sequencing of Ranaviruses Isolated from Edible Frogs (
Pelophylax esculentus
)
Authors: Ariel Ellen , Subramaniam Kuttichantran , Imnoi Kamonchai , Sriwanayos Preeyanan , Ahasan M. Shamim , Olesen Niels J. , Amedeo Manfrin , Toffan Anna , Waltzek Thomas B. 
Published: September 2017

ABSTRACT

Ranaviruses were isolated from wild edible frogs (
Pelophylax esculentus
) during epizootics in Denmark and Italy. Phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the
Andrias davidianus
ranavirus (ADRV), common midwife toad ranavirus (CMTV),
Testudo hermanni
ranavirus (THRV), and pike-perch iridovirus (PPIV).
Complete Genome Sequence of Actinobacillus pleuropneumoniae Strain KL 16 (Serotype 1)
Authors: Park Byung-Sun , Han Jemin , Shin Dong-Ju , Jeong Young-Ju , Lee Nakhyung 
Published: September 2017

ABSTRACT

Actinobacillus pleuropneumoniae
is a bacterial pathogen causing highly contagious porcine pleuropneumonia. Due to limited information on this species, it is difficult to study the biology of
A. pleuropneumoniae
at the genome level. Here, we report the fully annotated genome sequence of
A. pleuropneumoniae
strain KL 16.
Genetic Tools for the Enhancement of Probiotic Properties
Authors: Ortiz-Velez Laura , Britton Robert 
Published: September 2017

ABSTRACT

The
Lactobacillus
genus is a diverse group of microorganisms, many of which are of industrial and medical relevance. Several
Lactobacillus
species have been used as probiotics, organisms that when present in sufficient quantities confer a health benefit to the host. A significant limitation to the mechanistic understanding of how these microbes provide health benefits to their hosts and how they can be used as therapeutic delivery systems has been the lack of genetic strategies to efficiently manipulate their genomes. This article will review the development and employment of traditional genetic tools in lactobacilli and highlight the latest methodologies that are allowing for precision genome engineering of these probiotic organisms. The application of these tools will be key in providing mechanistic insights into probiotics as well as maximizing the value of lactobacilli as either a traditional probiotic or as a platform for the delivery of therapeutic proteins. Finally, we will discuss concepts that we consider relevant for the delivery of engineered therapeutics to the human gut.