bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive analysis identifies BRAF hotspot mutations associated with gliomas with peculiar epithelial morphology
Authors: Hatae Ryusuke , Hata Nobuhiro , Suzuki Satoshi O. , Yoshimoto Koji , Kuga Daisuke , Murata Hideki , Akagi Yojiro , Sangatsuda Yuhei , Iwaki Toru , Mizoguchi Masahiro , Iihara Koji 
Published: October 2016

Brain tumors harbor various BRAF alterations, the vast majority of which are the BRAF kinase‐activating V600E mutation. BRAF mutations are most frequently detected in certain subtypes of low‐grade glioma, such as pilocytic astrocytoma (PA), pleomorphic xanthoastrocytoma (PXA), ganglioglioma (GG) and dysembryoplastic neuroepithelial tumor (DNT). However, it is unclear whether gliomas harboring BRAF mutations can be invariably regarded as these glioma subtypes or their derivatives. To address this question, we analyzed 274 gliomas in our institutional case series. We performed high‐resolution melting analyses and subsequent direct Sanger sequencing on DNA isolated from snap‐frozen tumor tissues. As expected, BRAF mutations were detected in the aforementioned low‐grade gliomas: in 4/27 PAs, 2/3 PXAs, 4/8 GGs, and 1/6 DNTs. In addition to these gliomas, 1/2 astroblastomas (ABs) and 2/122 glioblastomas (GBs) harbored BRAF mutations. Pathological investigation of the two GBs revealed that one was a GB displaying epithelial features that presumably arose from a precedent GG, whereas the other GB, which harbored a rare G596 A mutation, showed marked epithelial features, including astroblastic rosettes. Our results indicate that in addition to being present in established BRAF‐associated gliomas, BRAF mutations might be associated with epithelial features in high‐grade gliomas, including sheet‐like arrangement of polygonal tumor cells with a plump cytoplasm and astroblastic rosettes, and thus could potentially serve as a genetic marker for these features.
A very human being: Sister Marie Simone Roach, 1922–2016
Authors: Villeneuve Michael J , Tschudin Verena , Storch Janet , Fowler Marsha D M , Peter Elizabeth 
Published: October 2016

Sister (Sr.) Marie Simone Roach, of the Sisters of St. Martha of Antigonish, Nova Scotia, died at the Motherhouse on 2 July 2016 at the age of 93, leaving behind a rich legacy of theoretical and practical work in the areas of care, caring and nursing ethics. She was a humble soul whose deep and scholarly thinking thrust her onto the global nursing stage where she will forever be tied to a central concept in nursing, caring, through her Six Cs of Caring model. In Canada, she was the lead architect of the Canadian Nurses Association's first code of ethics, and her influence on revisions to it is still profound more than 35 years later. In this paper, four global scholars in nursing and ethics are invited to reflect on Sr. Simone's contribution to nursing and health‐care, and we link her work to nursing and health‐care going forward.
Simulated human patients and patient‐centredness: The uncanny hybridity of nursing education, technology, and learning to care
Authors: Ireland Aileen V. 
Published: October 2016

AbstractPositioned within a hybrid of the human and technology, professional nursing practice has always occupied a space that is more than human. In nursing education, technology is central in providing tools with which practice knowledge is mobilized so that students can safely engage with simulated human patients without causing harm to real people. However, while there is an increased emphasis on deploying these simulated humans as emissaries from person‐centred care to demonstrate what it is like to care for real humans, the nature of what is really going on in simulation—what is real and what is simulated—is very rarely discussed and poorly understood. This paper explores how elements of postcolonial critical thought can aid in understanding the challenges of educating nurses to provide person‐centred care within a healthcare culture that is increasingly reliant on technology. Because nursing education is itself a hybrid of real and simulated practice, it provides an appropriate case study to explore the philosophical question of technology in healthcare discourse, particularly as it relates to the relationship between the human patient and its uncanny simulated double. Drawing on postcolonial elements such as the uncanny, diaspora, hybridity, and créolité, the hybrid conditions of nursing education are examined in order to open up new possibilities of thinking about how learning to care is entangled with this technological space to assist in shaping professional knowledge of person‐centred care. Considering these issues through a postcolonial lens opens up questions about the nature of the difficulty in using simulated human technologies in clinical education, particularly with the paradoxical aim of providing person‐centred care within a climate that increasingly characterized as posthuman.
The effect of longitudinal chromatic aberration on the lag of accommodation and depth of field
Authors: Jaskulski Mateusz , Marín‐Franch Iván , Bernal‐Molina Paula , López‐Gil Norberto 
Published: October 2016

AbstractPurposeLongitudinal chromatic aberration is present in all states of accommodation and may play a role in the accommodation response and the emmetropisation process. We study the change of the depth of field (DOFi) with the state of accommodation, taking into account the longitudinal chromatic aberration.MethodsSubjective DOFi was defined as the range of defocus beyond which the blur of the target (one line of optotypes of 0.1 logMAR shown on a black‐and‐white microdisplay, seen through different colour filters) was perceived as objectionable. The subject's eye was paralysed and different, previously‐measured accommodative states (corresponding to the accommodative demands of 0D, 2D and 4D) were simulated with a deformable mirror. Different colour conditions (monochromatic red, green and blue and polychromatic (white) were tested. The DOFi was measured subjectively, using a motorised Badal system.ResultsTaking as reference the average accommodative response for the white stimulus, the blue response exhibits on average a lead of 0.45 ± 0.09D, the green a negligible lead of 0.07 ± 0.02D and red a lag of 0.49 ± 0.10D. The monochromatic DOFi, calculated by averaging DOFi over the red, green and blue colour conditions for each accommodative demand was 1.10 ± 0.10D for 0D, 1.20 ± 0.08D for 2D, and 1.26 ± 0.40D for 4D. The polychromatic white DOFi were greater than the average monochromatic DOFi by 19%, 9% and 14% for 0D, 2D, and 4D of accommodative demand, respectively.ConclusionThe longitudinal chromatic aberration causes a dioptric shift of the monochromatic accommodation response. The study did not reveal this shift to depend on the accommodative demand or to have an effect on the DOFi.
Collaborative care of non‐urgent macular disease: a study of inter‐optometric referrals
Authors: Ly Angelica , Nivison‐Smith Lisa , Hennessy Michael P. , Kalloniatis Michael 
Published: October 2016

AbstractPurposeDiseases involving the macula and posterior pole are leading causes of visual impairment and blindness worldwide and may require prompt ophthalmological care. However, access to eye‐care and timely patient management may be limited due to inefficient and inappropriate referrals between primary eye‐care providers and ophthalmology. Optometrists with a special interest in macular disease may be useful as a community aid to better stratify and recommend best‐practice management plans for suitable patients. This study assesses such a notion by appraising the optometric referral patterns of patients with suspected macular disease to an intermediate‐tier optometric imaging clinic.MethodsWe performed a retrospective review of patient records and referrals using patients examined at Centre for Eye Health (CFEH) for an initial or follow up macular assessment between the 1/7/2013 and 30/6/2014 (n = 291). The following data were analysed: patient demographic characteristics, primary reason for referral, diagnosed/suspected condition, CFEH diagnosis and recommended management plan.ResultsThe number of referrals stipulating a diagnosis, confirmed after evaluation at CFEH was 121 of 291 (42%). After evaluation at CFEH, the number of cases without a specific diagnosis was approximately halved (reduced from 47% to 23%), while the number of cases with no apparent defect or normal aging changes rose from 1% to 15%. Overall diagnostic congruency for specified macular conditions was high (58–94%); cases were seldom (30/291, 10%) found to have a completely different macular condition. 244 of 291 (84%) patients seen at CFEH were recommended ongoing optometric care: either with the referring optometrist or through recall to CFEH. Referral to an ophthalmologist was recommended in 47 instances (16%).ConclusionsMore widespread adoption of intermediate‐tier optometric eye‐care referral pathways in macular disease (following opportunistic primary care screening) has the potential to reduce the number of cases with non‐specific diagnoses and to increase those with a diagnosis of normal aging changes or no apparent disease. The majority of cases seen under this intermediate‐tier model required ongoing optometric care only and did not require face‐to‐face consultation with an ophthalmologist.
Imaging the Propagation of the Electromechanical Wave in Heart Failure Patients with Cardiac Resynchronization Therapy
Authors: BUNTING ETHAN , LAMBRAKOS LITSA , KEMPER PAUL , WHANG WILLIAM , GARAN HASAN , KONOFAGOU ELISA 
Published: October 2016

BackgroundCurrent electrocardiographic and echocardiographic measurements in heart failure (HF) do not take into account the complex interplay between electrical activation and local wall motion. The utilization of novel technologies to better characterize cardiac electromechanical behavior may lead to improved response rates with cardiac resynchronization therapy (CRT). Electromechanical wave imaging (EWI) is a noninvasive ultrasound‐based technique that uses the transient deformations of the myocardium to track the intrinsic EW that precedes myocardial contraction. In this paper, we investigate the performance and reproducibility of EWI in the assessment of HF patients and CRT.MethodsEWI acquisitions were obtained in five healthy controls and 16 HF patients with and without CRT pacing. Responders (n = 8) and nonresponders (n = 8) to CRT were identified retrospectively on the basis of left ventricular (LV) reverse remodeling. Electromechanical activation maps were obtained in all patients and used to compute a quantitative parameter describing the mean LV lateral wall activation time (LWAT).ResultsMean LWAT was increased by 52.1 ms in HF patients in native rhythm compared to controls (P < 0.01). For all HF patients, CRT pacing initiated a different electromechanical activation sequence. Responders exhibited a 56.4‐ms ± 28.9‐ms reduction in LWAT with CRT pacing (P < 0.01), while nonresponders showed no significant change.ConclusionIn this initial feasibility study, EWI was capable of characterizing local cardiac electromechanical behavior as it pertains to HF and CRT response. Activation sequences obtained with EWI allow for quantification of LV lateral wall electromechanical activation, thus providing a novel method for CRT assessment.
Agronomic and genetic diversity in intermediate wheatgrass (Thinopyrum intermedium)
Authors: Jensen Kevin B. , Yan Xuebing , Larson Steve R. , Wang Richard R.‐C. , Robins Joseph G. 
Published: October 2016

AbstractIntermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] plant introductions (PI) have played a critical role in the development of improved intermediate wheatgrass cultivars. The objective of this study was to characterize a large number of intermediate wheatgrass populations over its native range for dry matter yield (DMY), crude protein (CP), in vitro true digestibility (IVTD), neutral detergent fibre (NDF), and rhizome development and genetic diversity and structure to identify superior populations and possible geographical regions in which to collect. Analysis of molecular variance using 643 AFLP bands partitioned 31% of the total genetic variation among these populations with 69% variance detected within populations. Bayesian cluster analysis identified two large groups designated as Asian and European under K = 2. In general, European accessions had greater DMY than did Asian accessions. Observed trends were towards greater CP values in populations with Asian ancestry. Selection index values above zero were observed in populations with European ancestry, and the exception was the late‐maturing (208 days to bloom) populations with Asian ancestry. Plant populations 383 551, 383 561, 401 161, 401 173, 440 021, 578 692 (cv. Tegmar) and 598 740 had rhizome scores greater than 7.0. Despite significant differences detected between populations (ΦST = 0.3136; P < 0.001), there was strong indication of admixed co‐ancestry or possible gene flow between K = 2–9 models.
Ecosystem carbon pools, fluxes, and balances within mature tallgrass prairie restorations
Authors: von Haden Adam C. , Dornbush Mathew E. 
Published: October 2016

AbstractTallgrass prairie restorations can quickly accrue organic C in soil and biomass, but the rate of C accumulation diminishes through time and is highly variable among more mature prairies. Long‐term soil organic carbon (SOC) accumulation in prairies has been linked to edaphic factors such as soil texture, soil moisture, and SOC content, but it is unclear how these factors affect the ecosystem processes that are responsible for observed differences in C accumulation rates in older prairies. We measured belowground plant and SOC pools and fluxes within 27–36‐year‐old restored tallgrass prairies in order to quantify total C storage, determine the net ecosystem production of C (NEP‐C), and explore which edaphic factors influence the ecosystem processes responsible for divergent NEP‐C. We found that 11% of organic C was stored in biomass, and we estimate that one‐third of post‐restoration C sequestration has occurred in biomass, thereby highlighting biomass as a large but often overlooked C pool. Belowground biomass and soil C pools were notably smaller than those reported for remnant prairie, suggesting that future belowground C accumulation could still occur. During this study, the prairies appeared to be a net source of C, although the range of NEP‐C values encompassed zero. Sand content positively affected NEP‐C via increased belowground biomass production‐C inputs, and SOC negatively affected NEP‐C due to increased soil respiration C outputs. However, soil moisture had a smaller negative effect on soil respiration, indicating that both SOC and soil moisture play important roles in determining prairie C balance.
Revisiting the Olympic Effect
Authors: Bista Rishav 
Published: October 2016

AbstractBy utilizing the log‐linear gravity model, other authors have found statistically robust, permanent and large effects of hosting mega‐events (e.g. Olympics) on international exports. Surprisingly, they found that the unsuccessful bidders to host the Olympics experienced a similar impact on exports. Utilizing alternate specification such as the Poisson pseudo‐maximum likelihood (PPML) estimation that allows for heteroskedasticity prevalent in trade data, the this paper fails to find a robust positive effect of hosting and bidding for a mega‐event on total aggregate exports. Under heteroskedasticity, the parameters of log‐linearized models estimated by ordinary least squares (OLS) lead to biased estimates of the true elasticities.
Gene‐environment correlations in the cross‐generational transmission of parenting: Grandparenting moderates the effect of child 5‐HTTLPR genotype on mothers' parenting
Authors: Kopala‐Sibley Daniel C. , Hayden Elizabeth P. , Singh Shiva M. , Sheikh Haroon I. , Kryski Katie R. , Klein Daniel N. 
Published: October 2016

AbstractEvidence suggests that parenting is associated cross‐generationally and that children's genes may elicit specific parenting styles (evocative gene‐environment correlation). This study examined whether the effect of children's genotype, specifically 5‐HTTLPR, on mothers' parenting behaviors was moderated by her own parenting experiences from her mother. Two independent samples of three‐year‐olds (N = 476 and 405) were genotyped for the serotonin transporter gene, and observational measures of parenting were collected. Mothers completed measures of the parenting they received as children. The child having a short allele on 5‐HTTLPR was associated with more maternal hostility (Samples 1 and 2) and with less maternal support (Sample 1), but only if the mother reported lower quality grandmothers' parenting (abuse and indifference in Sample 1 and lower levels of grandmother care in Sample 2). Results support the possibility of a moderated evocative gene‐environment correlation.
Electoral Linkages in Federal Systems: Barometer Voting and Economic Voting in the German Länder
Authors: Thorlakson Lori 
Published: October 2016

AbstractFederal systems create political competition at multiple territorial levels. While models of vertical bargaining conceptualise federal‐subnational relations as occurring between parties with exogenously defined interests, federalism also structures forms of interdependence between the federal and subnational levels. Political competition in multi‐level systems is marked by interdependence between the federal and subnational levels through barometer and second order voting effects. Findings of a more ‘autonomous’ form of political competition at the subnational level, through state‐level economic voting, are less common. This article examines Germany, a highly interdependent federation, to assess the extent to which voting in Land elections responds to Land level economic performance and whether political and institutional factors affect this. I find evidence that in Land level elections, voting for the federally incumbent party is responsive to federal economic performance. Alongside this, there is evidence of ‘uncoupled’ electoral behaviour at the Land level, with Land level economic voting. This is enhanced by single party government.
Phytochromes function as thermosensors inArabidopsis
Authors: Jung Jae-Hoon , Domijan Mirela , Klose Cornelia , Biswas Surojit , Ezer Daphne , Gao Mingjun , Khattak Asif Khan , Box Mathew S. , Charoensawan Varodom , Cortijo Sandra , Kumar Manoj , Grant Alastair , Locke James C. W. , Schäfer Eberhard , Jaeger Katja E. , Wigge Philip A. 
Published: October 2016

Combining heat and light responsesPlants integrate a variety of environmental signals to regulate growth patterns. Legriset al.and Junget al.analyzed how the quality of light is interpreted through ambient temperature to regulate transcription and growth (see the Perspective by Halliday and Davis). The phytochromes responsible for reading the ratio of red to far-red light were also responsive to the small shifts in temperature that occur when dusk falls or when shade from neighboring plants cools the soil.Science, this issue p.897, p.886; see also p.832
Complete Genome Sequence of IME207, a Novel Bacteriophage Which Can Lyse Multidrug-Resistant Klebsiella pneumoniae and
Salmonella
Authors: Liu Yannan , Mi Liyuan , Mi Zhiqing , Huang Yong , Li Puyuan , Zhang Xianglilan , Tong Yigang , Bai Changqing 
Published: October 2016

ABSTRACT

A novel lytic
Salmonella
bacteriophage was isolated by using
Klebsiella pneumoniae
as host cells. The phage's genome was determined to be 47,564 bp and has the highest similarity to
Salmonella
phage E1 and
Salmonella
phage 64795_sal3, with coverages of 61% and 56%, respectively. Here, we announce the phage's complete genome.
Complete Genome Sequences of Two Marine Vibrio cholerae Strains Isolated from the South Coast of Sweden
Authors: Thorell Kaisa , Collin Betty , Hernroth Bodil , Sjöling Åsa 
Published: October 2016

ABSTRACT

Vibrio cholerae
serogroups O1 and O139 are commonly associated with diarrhea, while non-O1-O139 strains may cause wound infections. Here, we present the genome sequences of two
V. cholerae
strains isolated from blue mussels (
Mytilus edulis
) collected in coastal waters of southern Sweden.
Complete Genome Sequence of Brucella abortus SKN 13 Isolated from Placenta of Aborted Cattle in Gujarat, India
Authors: Chauhan H. C. , Patel Bharat K. , Chandel B. S. , Patel Kirit B. , Patel A. C. , Shrimali M. D. , Patel S. S. , Bhagat A. G. , Rajgor Manish , Patel Mitul A. , Patel Maulik , Kala Jitendra , Patel Bhumika 
Published: October 2016

ABSTRACT

Brucella abortus
is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of
Brucella abortus
SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of
Brucella
field strains.
Genome Sequence of
Bluetongue virus
Serotype 17 Isolated in Brazil in 2014
Authors: Matos Ana Carolina Diniz , Rosa Júlio César Câmara , Nomikou Kyriaki , Guimarães Lorena Lima Barbosa , Costa Érica Azevedo , Guedes Maria Isabel Maldonado Coelho , Driemeier David , Lobato Zélia Inês Portela , Mertens Peter Paul Clement 
Published: October 2016

ABSTRACT

The complete genome sequence of
Bluetongue virus
(BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in 2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTV from the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from South America.
Draft Genome Sequence of the Cellulolytic Strain
Clostridium
sp. Bc-iso-3 Isolated from an Industrial-Scale Anaerobic Digester
Authors: Sun Li , Schnürer Anna 
Published: October 2016

ABSTRACT

Clostridium
sp. Bc-iso-3 is a cellulolytic strain isolated from a Swedish industrial-scale biogas digester. Here, we present the draft genome sequence of this strain, which consists of four contigs with a total length of 4,327,139 bp and an average coverage of 312.97×.
Draft Genome Sequence of Methyloligella halotolerans С2
T
, a New Halotolerant Methylotroph, Accumulating Poly-3-Hydroxybutyrate and Ectoine
Authors: Vasilenko Oleg V. , Doronina Nina V. , Shmareva Maria N. , Tarlachkov Sergey V. , Trotsenko Yuri A. 
Published: October 2016

ABSTRACT

Methyloligella halotolerans
С2
T
is a moderate halophilic obligate methylotroph, accumulating ultra-high-molecular-weight poly-3-hydroxybutyrate (up to 8 to 10 MDa) from methanol. Here we report a draft genome and annotation of
Methyloligella halotolerans
C2
T
(VKM B-2706
T
= CCUG 61687
T
= DSM 25045
T
).
Genome Sequence of the Multiple-Protease-Producing Strain Geobacillus thermoleovorans N7
,
a Thermophilic Bacterium Isolated from Paniphala Hot Spring, West Bengal, India
Authors: Bose Sucharita , Mukherjee Trinetra , Sen Urmimala , Roy Chayan , Rameez Moidu Jameela , Ghosh Wriddhiman , Mukhopadhyay Subhra Kanti 
Published: October 2016

ABSTRACT

Here, we present the draft genome sequence of
Geobacillus thermoleovorans
strain N7 (MCC 3175), isolated from Paniphala Hot Spring, West Bengal, India, which contains genes that encode several industrially and medically important thermostable enzymes like neutral protease, xylose isomerase, rhamnogalacturonan acetylesterase, nitrate and nitrite reductase,
l
-asparaginase, glutaminase, and RNase P
.
Complete Genome Sequence of Pigmentation-Negative Yersinia pestis Strain Cadman
Authors: Lovett Sean , Chase Kitty , Koroleva Galina , Palacios Gustavo , Rozak David , Ladner Jason T. 
Published: October 2016

ABSTRACT

Here, we report the genome sequence of
Yersinia pestis
strain Cadman, an attenuated strain lacking the
pgm
locus.
Y. pestis
is the causative agent of plague and generally must be worked with under biosafety level 3 (BSL-3) conditions. However, strains lacking the
pgm
locus are considered safe to work with under BSL-2 conditions.