bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly tamoxifen-inducible principal-cell-specific Cre mice with complete fidelity in cell specificity and no leakiness
Authors: Chen Lihe , Gao Chao , Zhang Long , Zhang Ye , Chen Enuo , Zhang Wenzheng 
Published: December 2017

AbstractAn ideal inducible system should be cell-specific and have absolute no background recombination without induction (i.e. no leakiness), a high recombination rate after induction, and complete fidelity in cell specificity (i.e. restricted recombination exclusively in cells where the driver gene is expressed). However, such an ideal mouse model remains unavailable for collecting duct research. Here, we report a mouse model that meets these criteria. In this model, a cassette expressing ERT2CreERT2 (ECE) is inserted at the ATG of the endogenous Aqp2 locus to disrupt Aqp2 function and to express ECE under the control of the Aqp2 promoter. The resulting allele is named Aqp2ECE. There was no indication of a significant impact of disruption of a copy of Aqp2 on renal function and blood pressure control in adult Aqp2ECE/+ heterozygotes. Without tamoxifen, Aqp2ECE did not activate a Cre-dependent red fluorescence protein (RFP) reporter in adult kidneys. A single injection of tamoxifen (2 mg) to adult mice enables Aqp2ECE to induce robust RFP expression in the whole kidney 24h post injection, with the highest recombination efficiency of 95% in the inner medulla. All RFP-labeled cells expressed principal cell markers (Aqp2 & Aqp3), but not intercalated cell markers (V-ATPase B1B2, and carbonic anhydrase II). Hence, Aqp2ECE confers principal cell-specific tamoxifen-inducible recombination with absolute no leakiness, high inducibility, and complete fidelity in cell specificity, which should be an important tool for temporospatial control of target genes in the principal cells and for Aqp2+ lineage tracing in adult mice.
Ultrastructural analysis of intracellular membrane and microtubule behavior during mitosis ofDrosophilaS2 cells
Authors: Strunov Anton , Boldyreva Lidiya V. , Andreyeva Evgeniya N. , Pavlova Gera A. , Popova Julia V. , Razuvaeva Alena V. , Anders Alina F. , Renda Fioranna , Pindyurin Alexey V. , Gatti Maurizio , Kiseleva Elena 
Published: December 2017

AbstractS2 cells are one of the most widely usedDrosophila melanogastercell lines for molecular dissection of mitosis using RNA interference (RNAi). However, a detailed and complete description of S2 cell mitosis at the ultrastructural level is still missing. Here, we analyzed by transmission electron microscopy (TEM) a random sample of 144 cells undergoing mitosis, focusing on intracellular membrane and microtubule (MT) behavior. This unbiased approach allowed us to discover that S2 cells exhibit a characteristic behavior of intracellular membranes, involving the formation of a quadruple nuclear membrane in early prometaphase and its disassembly during late prometaphase. After nuclear envelope disassembly, the mitotic apparatus becomes encased by a discontinuous network of ER membranes that associate with mitochondria preventing their diffusion into the spindle area. We also observed a peculiar metaphase spindle organization. We found that kinetochores with attached k-fibers are almost invariably associated with lateral MT bundles that can be either interpolar bundles or k-fibers connected to a different kinetochore. This spindle organization is likely to favor chromosome alignment at metaphase and subsequent segregation during anaphase. In summary, we describe several previously unknown features of membrane and microtubule organization during S2 cell mitosis. The genetic determinants of these mitotic features of can now be investigated using an RNAi-based approach, which is particularly easy and efficient in S2 cells
Collective cell migration without proliferation: density determines cell velocity and wave velocity
Authors: Tlili Sham , Gauquelin Estelle , Li Brigitte , Cardoso Olivier , Ladoux Benoît , Delanoë-Ayari Hélène , Graner François 
Published: December 2017

AbstractCollective cell migration contributes to morphogenesis, wound healing or tumor metastasis. Culturing epithelial monolayers on a substrate enables to quantify such tissue migration. By using narrow strips, we stabilise the front shape; by inhibiting cell division, we limit density increase and favor steady migration; by using long strips, we observe a confined cell monolayer migrating over days. A coherent collective movement propagates over millimeters; cells spread and density decreases from the monolayer bulk toward the front. Cell velocity (∼micrometer per minute) increases linearly with cell radius, and does not depend explicitly on the distance to the front. Over ten periods of backwards propagating velocity waves, with wavelength ∼millimeter, are detected with a signal-to-noise ratio enabling for quantitative spatio-temporal analysis. Their velocity (∼ten micrometers per minute) is ten times the cell velocity; it increases linearly with the cell radius. Their period (∼two hours) is spatially homogeneous, and increases with the front density. When we inhibit the formation of lamellipodia, cell velocity drops while waves either disappear, or have a smaller amplitude and slower period. Our phenomenological model assumes that both cell and wave velocities are related with the activity of lamellipodia, and that the local stretching in the monolayer bulk modulates traction stresses. We find that parameter values close to the instability limit where waves appear yield qualitative and quantitative predictions compatible with experiments, including the facts that: waves propagate backwards; wave velocity increases with cell radius; lamellipodia inhibition attenuates, slows down or even suppresses the waves. Together, our experiments and modelling evidence the importance of lamellipodia in collective cell migration and waves.
The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor
Authors: Tseng Kuo-Fu , Wang Pan , Lee Yuh-Ru Julie , Bowen Joel , Gicking Allison M. , Guo Lijun , Liu Bo , Qiu Weihong 
Published: December 2017

AbstractIn animals and fungi, cytoplasmic dynein is a processive motor that plays dominant roles in various intracellular processes. In contrast, land plants lack cytoplasmic dynein but contain many minus-end-directed kinesin-14s. No plant kinesin-14 is known to produce processive motility as a homodimer. OsKCH2 is a plant-specific kinesin-14 with an N-terminal actin-binding domain and a central motor domain flanked by two predicted coiled-coils (CC1 and CC2). Here, we show that OsKCH2 specifically decorates preprophase band microtubulesin vivoand transports actin filaments along microtubulesin vitro. Importantly, OsKCH2 exhibits processive minus-end-directed motility on single microtubules as individual homodimers. We find that CC1 but not CC2 forms the coiled-coil for OsKCH2 dimerization. Instead, CC2 functions to enable OsKCH2 processivity by enhancing its binding to microtubules. Collectively, these results show that land plants have evolved unconventional kinesin-14 homodimers with inherent minus-end-directed processivity that may function to compensate for the loss of cytoplasmic dynein.
Government governance, executive networks and corporate investment efficiency
Authors: Jin Xin , Yu Junli 
Published: December 2017

PurposeOwing to the importance of the investment behavior in China, the purpose of this paper is to find the influence of executive network and government governance on investment efficiency.Design/methodology/approachThe paper use China’s listed companies as sample to make an investment efficiency determinant model.FindingsIn this article, the authors find that larger executive network and higher government governance will lead to more corporate investment efficient. Furthermore, the informal institution – executive network, is not only an effective way to alleviate financing constraints, but also can solve underinvestment problem. While the improvement of local government governance can provide institutional protection, it will also be more conducive to restrain overinvestment behavior.Research limitations/implicationsThe authors have not explored conduction path. Especially, the authors have not examined whether information spillover effect or the release of resources constraints in executive network plays a more important role to ease investment insufficient.Originality/valueUnder the Chinese circumstance, relationship governance can not only promote companies to improve investment efficiency, but also provide an important guarantee for sustained macroeconomic growth.
Evaluating the performance of e-government in developing countries
Authors: Deng Hepu , Karunasena Kanishka , Xu Wei 
Published: December 2017

Purpose
The purpose of this paper is to propose and validate a public value-based framework for evaluating the performance of electronic government (e-government), leading to the identification of the critical factors in creating public value through e-government in developing countries.

Design/methodology/approach
A comprehensive review of the related literatures is conducted for developing a public value-based framework. A survey of e-government users is conducted, and the survey data are analysed using structural equation modelling.

Findings
This study shows that information quality, e-services functionalities, user orientation, efficiency and openness of public organisations, equity, citizens’ self-development, trust, and environmental sustainability are the critical public values of e-government in developing countries. It reveals that the use of the public value concept is effective in evaluating the performance of e-government in developing countries.

Practical implications
This study provides a comprehensive investigation of the e-government performance for better understanding the value of e-government in developing countries. The findings can guide the development of e-government in developing countries.

Originality/value
This is the first study in testing and validating a public value-based framework for evaluating the performance of e-government in developing countries. It demonstrates how the concept of public value can be adopted for evaluating the performance of e-government.
Academics’ intention to adopt online technologies for public engagement
Authors: Dermentzi Eleni , Papagiannidis Savvas 
Published: December 2017

Purpose
The need for universities to connect with local communities and to make research relevant to the public has been highlighted over recent years through the debate about public engagement. At the same time, the internet and its applications have made it possible for universities and academics to engage with the public in an easier and more effective way. The purpose of this paper is to examine the factors that motivate academics to engage with the public online.

Design/methodology/approach
The decomposed theory of planned behaviour and uses and gratifications theory were used as a basis for the study’s research model. An online survey was conducted and 250 valid responses were used for the data analysis (structural equation modelling).

Findings
The results indicate that although academics seem to use online technologies for public engagement, this use takes the form of a one-way communication as the most influential factors of attitude when it comes to engaging with the public are image and information seeking rather than networking.

Originality/value
While there are some studies about the use of online technologies for teaching or for networking purposes within academia, little is known about academics’ intentions to engage with the public online. The study attempts to fill this gap and help universities understand their staff’s motivation and needs, which could be useful when it comes to launching successful public engagement campaigns.
Understanding Chinese consumer engagement in social commerce
Authors: Lin Jiabao , Li Lei , Yan Yanmei , Turel Ofir 
Published: December 2017

PurposeBuilding on the Chinese guanxi perspective, the purpose of this paper is to develop a theoretical model that explains the indirect effects of social support from friends on social commerce intentions, as mediated through the relational aspects that potential buyers develop with sellers.Design/methodology/approachHypotheses are tested with partial least squares (PLS)-graph applied to data collected via a survey of social media users (n=511). SPSS and PLS-graph are the statistical analysis tools used in this study.FindingsRelationship exists in social commerce interactions and its quality can be captured by swift guanxi and trust. These swift relationships matter as they drive users’ behavioral intentions on social commerce sites. The informational and social support people receive from friends helps in improving the relationship quality and can indirectly influence user behaviors on these sites.Research limitations/implicationsThis study has relied on a convenient sampling and this may limit the generalizability of the findings. Future research should employ broader and more random sampling techniques to re-validate and extend the findings.Originality/valueThe interpersonal aspect of relationship quality has received little attention in the social commerce literature. This study develops a theoretical model that explains consumers’ intention in social commerce. The findings reveal the mechanisms through which different types of social support indirectly influence social commerce intentions. They provide a unique glimpse into consumer behavior in Chinese settings, in which the guanxi aspect of relationship quality highly matters.
Exploring external auditors’ perceptions of the motivations behind management fraud in Egypt – a mixed methods approach
Authors: Kassem Rasha 
Published: December 2017

PurposeThis paper aims to explore the perceptions of external auditors’ on the motivations behind management fraud, which could increase the likelihood of detecting financial reporting fraud.Design methodology/approachMixed research methods, namely, an online questionnaire and semi-structured interviews, were used for data collection from external auditors in Egypt.FindingsThe findings revealed that the desire to get remuneration or bonuses and the need to secure financing are the most common motivations behind management fraud in Egypt. The current study also found other motivations behind management fraud that could be more specific to the Egyptian context.Research limitations/implicationsGaining access to small- and medium-size audit firms, in particular, was very difficult. This study did not use factor analysis to determine whether differences exist among the motives/factors, which should be the focus of future studies.Practical implicationsThis study was the first to provide empirical evidence on the motivations behind management fraud in the context of a developing nation such as Egypt. The findings of this study could be important for Egyptian regulators and external auditors who wish to reduce fraud risks in their own country. The findings could also help external auditors across the globe in recognising the signs that can trigger management motivations to engage in financial reporting fraud, which in turn could increase the likelihood of detecting financial reporting fraud.Originality/valueThis study was the first to provide empirical evidence on the motivations behind management fraud in the context of a developing nation such as Egypt. The findings of this study could be important for Egyptian regulators and external auditors who wish to reduce fraud risks in their own country. The findings could also help external auditors across the globe in recognising the signs that can trigger management motivations to engage in financial reporting fraud, which in turn could increase the likelihood of detecting financial reporting fraud.
Roots of social enterprise: entrepreneurial philanthropy, England 1600-1908
Authors: MacDonald Matthew , Howorth Carole 
Published: December 2017

Purpose
Insights into the roots of social enterprise from before the term was adopted are provided by examining histories of charitable service and comparing current understandings of social enterprise. Social enterprise models of welfare provision are evidenced from the seventeenth century onwards. Persistent themes are identified that provide insights for current practice and understanding.

Design/methodology/approach
This historiography examines interpretations from 1905 to the present day of examples of welfare provision between two watershed points: 1600, just prior to the Poor Laws and 1908, when the Old Age Pensions Act shifted emphasis in public sector provision.

Findings
Activities that would nowadays be termed social enterprise are evidenced in histories of charitable philanthropy covering each century since 1600. Prevailing attitudes uncritically demarcated deserving and undeserving poor. Histories contributed to a heroic narrative of social entrepreneurs, describing activities dependent on well-networked, politically active individuals that rarely continued beyond their involvement. The political environment was recognised to influence the types of organisations, governance and resourcing.

Research limitations/implications
The historiography takes examples from three centuries between 1600 and1908 but is not comprehensive. Recurrent themes are identified for further research.

Originality/value
Social enterprise is a twenty-first-century label but not a new phenomenon. Identification of prevailing themes provides insights for the understanding of social enterprises in the twenty-first century.
Institutionalising design thinking in social entrepreneurship
Authors: Kummitha Rama Krishna Reddy 
Published: December 2017

PurposeDesign thinking is attracting practitioners and researchers in various walks of life. User integration principle as part of design thinking is expected to transform product and service creation and delivery. Accordingly, various organisations that venture into design thinking develop customised solutions and serve people’s needs. Social enterprises, which are familiar for their superior social value creation, have also claimed to embark upon this innovative approach to address wicked problems. The current paper makes an attempt to analyse various social and organisational processes that promote social enterprises to adopt design thinking to create products, which are relevant and viable among user groups. This paper finds that by adopting design thinking, social enterprises portray their product innovation genre, thereby representing their passion in addressing wicked problems more effectively.Design/methodology/approachThis research adopts a qualitative case study method to understand the practice of design thinking in social enterprises.FindingsIn this study, it is found that that social enterprises adopt design thinking as a methodology while exploring solutions for stubborn problem space. However, neither communities nor social entrepreneurial teams are aware that they are carrying out design thinking practices. This research finds that social entrepreneurs and their teams take up designer roles and carry out designing processes by continuously consulting with user groups. One of the major reasons for the unintended adoption of design thinking is related to community integration and user participation in the day-to-day practices of social enterprises.Originality/valueThis study offers original research to understand social entrepreneurial exploration to adopt design thinking to create social value.
Research in the Social Enterprise Journal – from the margins to the mainstream
Authors: Doherty Bob 
Published: December 2017

Purpose
The purpose of this paper is to review the development and impact of the Social Enterprise Journal (SEJ) from its inception in 2005 until present day.

Design/methodology/approach
The paper analyses the thematic content of SEJ and its impact data from 2005 until 2017.

Findings
SEJ has broken new ground in the study of social enterprise (SE). It was the first journal back in 2005 to commence the exploration of this field and since has been the first to publish works on defining SE, their performance management, critical perspectives plus international differences. The paper shows that in the early years, SEJ was dominated by conceptual work aiming to understand SE plus their governance and performance management, which was mainly based on UK descriptive case studies and uncritical. By 2010, SEJ became established internationally with various issues being 100 per cent dominated by international aspects of SE. Recent more critical work has also enabled a process of “myth busting” in the SE field. This paper also shows the growth of SEJ in downloads and citations.

Practical implications
The paper demonstrates the important role SEJ has played in both improving practice and informing policy.

Originality/value
The paper is the first to review the development and impact of SEJ.
The Value of Access to Rail Transit in a Congested City: Evidence from Housing Prices in Beijing
Authors: Li Tao 
Published: December 2017

AbstractUsing unique data sets of Beijing's congestion patterns and housing prices, I find that consumers are willing to pay significantly more for access to rail transit in more congested areas. Transit accessibility, however, offers little travel advantage outside of dense urban areas. The expansion of the metro network mitigates the costs of road congestion, creating both private and social benefits. Two policy initiatives aimed at reducing congestion are found to have achieved positive value effects. Further analysis reveals heterogeneous demand for accessibility, with wealthier residents and those owning fewer cars paying a higher premium for access to rail transit.
The artefacts of death: CT post‐mortem findings
Authors: Sutherland Tom , O'Donnell Chris 
Published: December 2017

SummaryPost‐mortem CT is an increasingly used tool for investigating cause of death. While sharing many similarities with pre‐mortem imaging, a number of unique artefacts occur at post‐mortem CT, and these have the potential to mask underlying disease processes. It is vital that the artefacts associated with the process of dying and decompositional changes are recognised to avoid misdiagnosis. The following pictorial review discusses and illustrates the important and common post‐mortem changes.