bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of the effect of buckwheat substitute on the quality of nutritious and healthy biscuit production
Authors: Yıldırım Ali , Smail Amanj Nasih 
Published: October 2024

Abstract

Buckwheat, a nutritious pseudocereal, offers numerous health benefits including reducing celiac disease, high blood pressure, cholesterol, blood sugar regulation, and cancer risk reduction. Hence, the objective of this research was to create biscuits with enhanced proximate composition, physicochemical, nutritional, and sensory characteristics of biscuits using composite flours prepared with refined soft wheat flour (RWF) -based 0% (A), 30% (B), 50% (C), 70% (D) and 100% (E) buckwheat flour (BWF). Increase in BWF substitute resulted a significant (P ≤ 0.05) increase in moisture, ash, fat, and protein content, and a decrease in carbohydrate contents of both flours and biscuits. Increasing levels of BWF led to a decrease in L* and b* color values, and an increase in a* color value and pH of both flours and biscuits. Addition of BWF to RWF decreased the thickness, diameter and hardness values of the biscuits, while increased bulk density, weight, spread ratio and textural fracturability. All biscuits with different substitution levels of BWF had resulted with acceptable sensory characteristics. All sensory evaluations were found to be high scores for control sample and decreased with BWF supplementation. The use of BWF in biscuits not only enhances their nutritional, physicochemical, and functional properties but also promotes the production of healthier biscuits.
Humanitarian Standers of basic needs in temporary Displaced Camps in Shendi Locality
Authors: Ali Alhaj Saad Mohamed Ahmed , Khamees Rowyda Alnile Ahmad 
Published: October 2024

Abstract

Background: Minimum standards these are qualitative in nature and specify the minimum levels to be attained in humanitarian response regarding the provision location conditions, water, Sanitation and hygiene promotion.
Study design and Methods: cross-sectional- Sample survey and household based study was conducted on the displaced shelters in the temporary camps in Shendi locality, between August to October 2024 for the purpose of observe the minimum humanitarian standards in the shelter camps. Data was collected through face-to-face interviews through a written questionnaire (100 questionnaires were distributed to the displaced households heads in the camps) and also through the checklist and non-structural interview. The data was analyzed using SPSS software.
Results: It was noted that all displaced households are still residing in temporary shelter sites, which are schools, which made it possible to provide a number of ready-made facilities, such as toilets, a building, protection, and kitchens. In addition to the availability of water sources, as was shown through direct observation that the People who depend on tap water are 98%, and the capacity of storage containers ranges between 10- 40 liters/household. 66% of household have a good level of hygiene, While 78% of them have bad practices that could lead to water contamination. Regarding human waste it was found78% of people has flush toilets, 82% dispose of children’s faeces properly, 58% do not have slopes to dispose of sewage. 90% of people use open dumps to dispose of their waste, 96% of flies and mosquitoes breed in that waste. 48% of the insects observed were mosquitoes, and 98% use personal protection from the damage caused by these insects, 73.5% use repellents, 30% use mosquito nets, and 68% are tied in the evening.
Conclusion: The minimum humanitarian standards in the camps for the displaced are not sufficiently available in terms of quantity and quality, and this is a clear indicator of the spread of diseases related to these standards. The small percentage who have these standards are only due to their own efforts, which requires more concerted efforts by the government and organizations to provide These standards are likely to become a hotspot for the spread of epidemics if this trend continues.
Parasite clearance and protection from Plasmodium falciparum infection (PCPI): a two-arm, parallel, double-blinded, placebo-controlled, randomised trial of presumptive sulfadoxine-pyrimethamine versus artesunate monotherapy among asymptomatic children 3-5 years of age in Zambia
Authors: Martinez-Vega Rosario , Chaponda Mike , Mousa Andria , Gwasupika Jonathan , Chaponda Enesia Banda , Daka Victor , Mwanza Sydney , Chipunga Mukuka , Beshir Khalid B. , Chopo-Pizarro Ana , Kaur Harparkash , Okell Lucy , Hansson Helle , Hocke Emma Filtenborg , Alifrangis Michael , Gosling Roland , Roper Cally , Sutherland Colin , Chico R Matthew 
Published: October 2024

Abstract

Background: The 2022 malaria chemoprevention guidelines of the World Health Organization (WHO) recommend the provision of a full treatment course of an antimalarial medicine at predefined intervals, regardless of whether the child is infected with malaria, to prevent illness in moderate to high perennial malaria transmission settings. Sulfadoxine-pyrimethamine (SP) is usually used for this intervention, now called perennial malaria chemoprevention (PMC). The K540E mutation in the dihydropteroate synthase (dhps) gene circulating in Africa is thought to be associated with treatment failure and reduced chemoprevention efficacy in children but the exact effect remains unclear.
Methods: This protocol is for a two-arm, parallel, double-blind, placebo-controlled, randomised trial in Zambia that is designed to evaluate the effect of parasite genotypes on the efficacy of single-dose SP among asymptomatic children between 3-5 years of age. Children are randomly allocated to one of two groups for directly observed treatment. Over seven consecutive days (days -7 to -1), children in the SP group (n=400) receive placebo artesunate (AS), then active SP (day 0). In contrast, children in the AS group (n=200) receive active artesunate for seven consecutive days, followed by placebo SP (day 0). Then, on days 0, 2, 5, 7, and weekly thereafter until day 28, children provide blood for thick smear slides. Dried blood spots are collected on the same days and weekly from day 28 to day 63 for quantitative polymerase chain reaction (qPCR) and genotype analyses using a platform based on PCR followed by targeted next-generation sequencing.
Discussion: Our aim is to measure the effect of the parasite genotypes associated with SP resistance on parasite clearance and protection from new infection when exposed to SP chemoprevention. We will report unblinded results including: (i) time-to-parasite clearance among SP recipients who were positive on day 0 by qPCR and measured to day 63; (ii) mean duration of SP protection against infection, and (iii) mean duration of symptom-free status among SP recipients who were parasite free on day 0 by qPCR. Our conclusions will reflect on the utility of WHO’s new malaria chemoprevention efficacy study protocol with its follow-up to day 28 versus day 63.
Trial Registration: ClinicalTrials.gov NCT06166498; 11/12/2023.
Identification by immunoinformatics of the B and T epitopes in F and HN proteins of Newcastle disease viruses isolated in Madagascar
Authors: Tendrinarisoa Randriamamisolonirina Natolotriniavo , Suzanne Razafindrafara Mirantsoa , Abel Andriantsimahavandy Andriambandaina , Fridolin Maminiaina Olivier 
Published: October 2024

Abstract

Although conventional vaccines are widely used, outbreaks of Newcastle disease (ND) are still observed in Madagascar. This situation encourages the search for a more effective vaccine against this disease. Therefore, this study aims to develop a "multi-epitopic vaccine" based on circulating strains of Newcastle disease virus (NDV). To achieve this, it focuses on identifying B and T epitopes present in the F and HN proteins of 12 NDV strains isolated in Madagascar, using immunoinformatics methods. The sequences of F and HN proteins are extracted from the NCBI database and analyzed using specialized servers such as Bepipred v2.0, NetMHIIpan 4.0, and NetCTL v1.2 to predict the epitopes. These epitopes are then evaluated for antigenicity, non-allergenicity, and localization in exo-membrane regions, using tools such as VaxiJen 2.0, AllerTop v2.0, and TMHMM v2.0. The results reveal the presence of 52 epitopes in the F proteins and 68 epitopes in the HN proteins, all located in the exo-membrane regions of NDV. These epitopes prove to be antigenic and non-allergenic, offering promising potential for the development of a multi-epitopic vaccine.
Agile and Design Thinking in Pharma and Healthcare: A Systematic Review of Requirement Gathering and User Adoption
Authors: Sahadev Chaitanya 
Published: October 2024

Abstract

Purpose - Agile and Design Thinking methodologies became popular in the highly-regulated pharmaceutical and healthcare industry for overcoming such obstacles as requirements gathering, regulatory compliance, and product adoption by users. The literature on their intersection is, however, fragmented. The purpose of this paper is to systematically review the intersection of Agile and Design Thinking and their influence on user story development and product adoption in pharmaceutical and healthcare product management.
Design/Methodology/Approach - A systematic literature review was conducted, analyzing the use of Agile and Design Thinking methodologies in healthcare and pharmaceutical product management, with a focus on requirement gathering and user adoption.
Findings - The results elucidate that the iterative repackaging of agile practices and a user-centric theme in design thinking principles, increase the positive effects on product development outcomes. That said, the advent of standardized user stories which meet reg & bus requirements has been difficult for many and in general a heavy application of lightly enforced requests/resistance → leading to poor user adoption rates.
Research Limitation/Implications - The current review identifies literature gaps in the integration of Agile and design thinking methodologies specifically within a regulatory regime like those found within pharmaceutical and healthcare. Further studies should provide standardized guidelines for regulatory compliance and user-centric requirement gathering.
Originality/Value – This study provides an insight of Agile and Design Thinking integration in regulated product management therefore offering grounds for newer gap in literature, to consider. The focus is on recent trends in the field, challenges faced within the area and gaps in current research at the intersection of these methodologies with healthcare and pharmaceutical product management.
Paper type – Literature review
Bioaccumulation pattern of heavy metals in Avicennia marina at Visakhapatnam and Coringa mangroves in India
Authors: Kambala Anand Raju , Chintala Ramakrishna , Reka Srinivas 
Published: October 2024

Abstract

This study explores the impact of heavy metal accumulation on Avicennia marina's physiological and anatomical aspects, focusing on its capacity for metal uptake and tolerance. Conducted across the Visakhapatnam Entrance Channel (VEC) field, Control Coringa mangroves, and controlled ex-situ greenhouse environments, the study examines the accumulation patterns of chromium (Cr), lead (Pb), and cadmium (Cd). The Potential Ecological Risk Index (RI) indicated that Cd posed a very high risk, with Cr and Pb also showing significant risks. Heavy metal concentrations in the VEC from Cr (36.58–76.41 µg/g), Mn (209.19–428.8 µg/g), Cu (29.91–45.56 µg/g), Zn (306.44–925.16 µg/g), Cd (2.49–4.65 µg/g), and Pb (42.0–155.64 µg/g). The potential ecological risk coefficient Eir consistently placed Cd in the high-risk category, with other metals generally in low to moderate-risk categories. Physiological changes in plant tissues were analyzed using a fluorescence microscope, and higher metal concentrations were assessed with Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The Bioconcentration Factor (BCF) and Translocation Factor (TF) were calculated to evaluate metal accumulation and translocation efficiency. In VEC, significant anatomical changes in Avicennia marina included moist leaves, expanded mesophyll areas, and thick cuticles with heavy metal deposits, especially in high metal concentrations. The region's pollution, driven by port activities and nearby industries, elevated heavy metal levels in sediments. Cd was identified as a very high-risk element at all stations, while other metals were categorized under low or moderate risk. Comparative analysis with the Control Coringa mangroves indicated potential variations in metal accumulation strategies between the two regions within the same species. Anatomical changes in the VEC were more pronounced than fewer disruptions in Coringa mangroves, suggesting differential adaptive responses to environmental stressors. This study underscores the need for targeted environmental management strategies to mitigate heavy metal contamination and highlights the importance of maintaining healthy mangrove habitats amidst increasing anthropogenic pressures.
Identifying Mechanism of RSV for the Treatment of COVID-19 and Idiopathic Pulmonary Fibrosis via Suppressing Inflammation Response Through IL-17 Signaling Pathway from the Perspectives of in silico Study
Authors: Wang Jiahao , Shi Jiamiao , Jia Ning , Sun Qinru 
Published: October 2024

Background:
Both coronavirus disease 2019 (COVID-19) and idiopathic pulmonary
fibrosis (IPF) could cause severe pulmonary injury and have extremely dismal
prognoses with a high risk of mortality. Resveratrol (RSV), a natural polyphenol, has
promising potential in the treatment of viral infection and pulmonary fibrosis.

Objective:
The purpose of this research was to investigate the unclear mechanism of RSV
as an anti-COVID-19 and IPF therapy.

Method:
Utilizing relevant databases, the intersection of genes related to IPF, COVID-19,
and possible RSV targets was discovered. Then the obtained targets were investigated using
GO and KEGG analysis, TP and PPI network analysis. Furthermore, the binding affinities
between core targets and RSV were calculated using molecular docking.

Results:
The 1101 COVID-19 targets, 2166 IPF targets, and 341 RSV targets intersected
with 21 overlapping targets. PPI network reveals the interactions among targets and TP
network reveals interactions between targets and pathways. Five targets including JUN,
CCL2, CXCL8, IL6, and SERPINE1 were identified as the core targets through two network
analyses. GO analysis demonstrated chemotaxis, inflammatory response and angiogenesis
were the significant pathophysiological processes. Combing TP network analysis
and KEGG analysis, IL-17 signaling pathway was considered as the significant pathway.
Except for JUN, molecular docking showed the binding energies of other four targets
were lower than -5 kcal/mol indicating intimate interactions between RSV and other targets.

Conclusions:
Our research elucidate the targets, pathways and pathophysiological processes
of RSV involved in effects of anti-COVID-19 and IPF, suggesting RSV could be a therapeutic
candidate for reducing infection and fibrosis.
FOXN3-AS1: A Candidate Prognostic Marker and Epigenetic Target with Immunotherapeutic Implications in Acute Myeloid Leukemia
Authors: Ge Fangfang , Wang Yulu , Chen Peng , Sharma Amit , Huang Xiaoli , Dakal Tikam Chand , Wang Zifeng , Jaehde Ulrich , Essler Markus , Schmid Matthias , Schmidt-Wolf Ingo G.H. 
Published: October 2024

Aim:
We focused on the FOXN3 gene and selected its antisense transcripts
(FOXN3-AS1) to investigate its potential involvement in acute myeloid leukemia (AML).

Background:
Several integrated multi-omics datasets have expanded the horizons of the cancer
landscape. With the emergence of new high-throughput technologies, a large number of
non-coding RNAs have been confirmed to be involved in the pathogenesis of different types of
hematological malignancies.

Methods:
We conducted experimental validation using quantitative polymerase chain reaction
(qPCR) with bone marrow specimens from AML patients. Then, Kaplan-Meier (KM) and Receiver
Operating Characteristic (ROC) curves were used to substantiate the prognostic association
between FOXN3-AS1 and AML patients within the TCGA database. Correlation between
FOXN3-AS1 expression and gene mutation, immune, and immune function using Spearman
correlation analysis. To explore the physical and functional interaction between FOXN3-AS1
and the DNMT1 protein, we utilized the RPISeq web tool from Iowa State University. Subsequently,
we performed qPCR experiments to test the effect of 5AzaC (DNMT1 inhibitor) on
FOXN3-AS1 expression AML cell lines (THP1 and OCI-AML3). We leveraged the “OncoPredict”
R package in conjunction with the Genomics of Drug Sensitivity (GDSC) database to predict
drug response in AML patients expressing FOXN3-AS1.

Results:
We observed a significant upregulation of FOXN3-AS1 expression in AML patients
compared to healthy controls using clinical samples. The TCGA database revealed an association
between high FOXN3-AS1 expression and adverse prognosis. In our subsequent analysis,
genes with poor prognostic implications in AML patients were exclusively identified in the
FOXN3-AS1 high-expression group, further corroborating this relationship. AML patients
with higher FOXN3-AS1 expression levels may respond less optimally to immunotherapy than
patients with lower levels. Besides, we computationally predicted the interaction of FOXN3-
AS1 and DNMT1 protein and experimentally confirmed that DNMT1i (GSK-3484862) affects
the expression level of FOXN3-AS1. We also found that the chemotherapy drugs (5-Fluorouralic,
Cisplatin, Dactolisib, Sapitinib, Temozolomide, Ulixertinib, Vinorelbine, Ruxolitinib, Osimertinib
and Cisplatin) showed favorable responses in AML patients with high FOXN3-AS1
expression levels.

Conclusion:
Our candidate approach identifies FOXN3-AS1 as a prognostic indicator of survival
in AML with a potential immune-related role. The preliminary observations we made on
FOXN3-AS1/DNMT1 crosstalk warrant more in-depth invested immunotherapeutic approaches
in AML.
Synthesis, DFT, ADMET, and Docking studies of Novel Sulfonyl Piperidine Analogues containing 2,3-Dihydrobenzofuran-5-Carboxamide
Authors: Reddy Tummuri Sudheer , Raja Karreddula , Pitchika Gopi Krishna , Surendra Babu Manubolu Surya 
Published: October 2024

Background:
The development of effective anti-cancer medicines with low
side effects is imperative as cancer continues to be a leading cause of death globally. By
obstructing the survival and growth of cancer cells, small-molecule medications have
made tremendous progress in the field of cancer research. Several bioactive heterocyclic
compounds, including derivatives of piperidine and 2,3-dihydrobenzofuran, have shown
great promise and are found in various anti-cancer medications. Cancer growth and metastasis
are hindered by these small molecule inhibitors, which interfere with vital signals
that drive cancer cell proliferation.

Objective:
This study focuses on the synthesis and evaluation of novel Sulfonyl Piperidine
Analogues containing 2,3-Dihydrobenzofuran-5-Carboxamide as potential anti--
cancer agents.

Methods:
The synthesized compounds were characterized using spectroscopic techniques
such as 1H NMR and ESI-MS. Protein-drug interaction studies, DFT analysis,
and target prediction techniques were employed. The anti-cancer properties of the compounds
were evaluated in vitro against MCF-7 cell lines. Compounds 5 and 7 were specifically
investigated for their growth-inhibitory effects on MCF7 breast cancer cells.

Results:
Compounds5 and 7 demonstrated strong binding affinity towards both mutated
BRCA1 (PDB ID: 1N5O) and BRCA2 (PDB ID:8BR9). Furthermore, they displayed
notable efficacy against MCF-7 cell lines.

Conclusion:
Synthesized compounds displayed activity against MCF-7 cell lines, supporting
findings from in-silico predictions. Further investigations are warranted to elucidate
the mechanisms of action of these selected molecules against MCF-7 cell types.
From Proteomics to Diagnosis: Biomarker Discovery in Tuberculosis Research
Authors: Yang Jiarong , Zheng Jianhua 
Published: October 2024

Abstract:
Tuberculosis (TB) is a leading cause of death from a single infectious disease
worldwide. Early and accurate diagnosis is advantageous for timely detection and
prompt treatment, thereby reducing the risk of disease transmission, which is essential
for effective TB control. Biomarkers provide a valuable resource for TB diagnosis. Proteomic
technologies have emerged as a powerful tool in biomarker discovery. In this perspective,
we explore how proteomic technologies contribute to the discovery of TB diagnostic
biomarkers. We also address the challenges and discuss prospective methods to
augment the performance of biomarkers in diagnosing TB.
Protective Effect of Aloe-emodin on Cognitive Function in Copper-loaded
Rats Based on The Inhibition of Hippocampal Neuron Ferroptosis
Authors: Wang Xie , Chen Hong , Shao Nan , Zhang Xiaoyan , Huang Chenye , Li Xiangjun , Zhang Juan , Chang Ze , Tang Le , Xie Daojun 
Published: October 2024

Background:
Aloe-emodin (AE), a monomer derived from traditional Chinese
medicine, has demonstrated remarkable efficacy in the clinical management of cognitive disorders.
Ferroptosis (FPT), a specialized form of programmed cell death, plays a critical role in the
pathological progression of various cognitive diseases.

Methods:
This study explored the therapeutic potential of AE in a rat model of Wilson's disease
cognitive impairments (WDCI) and examined whether these effects are mediated through the silencing
information regulator 1 (SIRT1)-regulated FPT signaling pathway. Employing techniques,
such as the Morris water maze (MWM), Hematoxylin & eosin (H&E) staining, Transmission electron
microscopy (TEM), Immunofluorescence (IF), assessments of oxidative stress markers, and
measurements of FPT-related protein levels, we evaluated the extent of SIRT1-mediated FPT and
the therapeutic efficacy of AE.

Results:
The findings from the WD copper-loaded rat model experiments revealed that MWM,
H&E, TEM, and IF outcomes indicated AE's potential to promote the restoration of learning and
memory functions, ameliorate hippocampal neuronal morphological damage, and preserve cell
membrane integrity. Results from western blot (WB) and ELISA analyses demonstrated that AE
markedly upregulated the expression of SIRT1, nuclear factor erythroid-2-related factor 2 (Nrf2),
solute carrier family 7 member 11 (SCL7A11), and glutathione peroxidase 4 (GPX4) proteins
while simultaneously reversing the expression of oxidative stress markers such as malondialdehyde
(MDA), glutathione (GSH), and superoxide dismutase (SOD), and reactive oxygen species
(ROS). Consequently, we posit that AE may attenuate WD copper-loaded rat model hippocampal
neuronal FPT by activating the SIRT1-mediated signaling pathway.

Conclusion:
These findings suggested that AE mitigates WD copper-loaded rat model hippocampal
neuronal damage through the activation of SIRT1-mediated FPT, thereby presenting a valuable
candidate Chinese herbal monomer for the clinical treatment of WDCI.