bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling the effect of three‐dimensional bioactive fibre mesh scaffolds functionalized with silanol groups on bacteria growth
Authors: Rodrigues Ana I. , Franco Albina R. , Rodrigues Fernando J. , Leonor Isabel B. , Reis Rui L. 
Published: April 2016

AbstractThe need to replace or repair deteriorating bones and simultaneously prevent the formation of bacteria biofilm without impairing local tissue integration has pushed scientists to look for new designs and processing methods to develop innovative biomaterials. Silicon‐based biomaterials, widely studied for application in bone regeneration, have demonstrated antibacterial properties. Herein, the aim of this work is to investigate the potential of the functionalization of biomaterials surfaces with silanol groups to prevent the bacterial biofilm formation. For that, we evaluated the adherence and biofilm formation ofEscherichia coli(E. coli, Gram negative) andStaphylococcus aureus(S. aureus, Gram positive) on starch‐based scaffolds. Three‐dimensional fibre meshes scaffolds were developed by wet‐spinning and functionalized with silanol (Si—OH) groups using a calcium silicate solution as a nonsolvent. The functionalization of the scaffolds was confirmed by X‐ray photoelectron spectroscopy. The developed scaffolds showed no biocide activity against the bacterial tested, although the colony‐forming units (CFU) mL−1counts were significant lower between 4 and 12 h of incubation for both bacteria. The adherenceof E. coliandS. aureusto the scaffolds was also investigated. After a growth period of 12 h, the SPCL scaffolds functionalized with Si—OH groups showed a reduced bacterial adherence ofE. coliandS. aureus. The functionalized scaffolds showed a positive effect in preventing the formation of biofilm in the case ofS. aureus, however, in the case ofE. colithis was not observed, suggesting that silanol groups may only have a positive effect in preventing the proliferation of gram‐positive bacteria. Thein vitrobiological assessment of the functionalized materials showed that these materials sustained cell proliferation and induced their osteogenic differentiation. The outcome of this work suggests that the presence of Si—OH groups in SPCL scaffolds maintained bactericidal activity againstS. aureus. Further research is still needed in order to understand the full antibacterial potential of Si—OH groups. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2189–2199, 2016.
Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene
Authors: Lamichhane Sujan , Anderson Jordan A. , Remund Tyler , Sun Hongli , Larson Mark K. , Kelly Patrick , Mani Gopinath 
Published: April 2016

AbstractIn this study, the effect of different structures (flat, expanded, and electrospun) of polytetrafluoroethylene (PTFE) on the interactions of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets was investigated. In addition, the mechanisms that govern the interactions between ECs, SMCs, and platelets with different structures of PTFE were discussed. The surface characterizations showed that the different structures of PTFE have the same surface chemistry, similar surface wettability and zeta potential, but uniquely different surface topography. The viability, proliferation, morphology, and phenotype of ECs and SMCs interacted with different structures of PTFE were investigated. Expanded PTFE (ePTFE) provided a relatively better surface for the growth of ECs. In case of SMC interactions, although all the different structures of PTFE inhibited SMC growth, a maximum inhibitory effect was observed for ePTFE. In case of platelet interactions, the electrospun PTFE provided a better surface for preventing the adhesion and activation of platelets. Thus, this study demonstrated that the responses of ECs, SMCs, and platelets strongly dependent on the surface topography of the PTFE. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2291–2304, 2016.
Diphenylalanine peptide nanotubes self‐assembled on functionalized metal surfaces for potential application in drug‐eluting stent
Authors: Zohrabi Tayebeh , Habibi Neda , Zarrabi Ali , Fanaei Maryam , Lee Lai Yeng 
Published: April 2016

AbstractThis study focuses on the potential of diphenylalanine self‐assembled peptide nanotubes (FF Nts) for delivery of flufenamic acid (FA) from metal implants. Self‐assembly of FF Nts was studied in solution and on surfaces of glass, silicone and gold substrates. FA was loaded inside the shell of FF Nts and subsequently FF/FA Nts were attached to gold surfaces. The substrate were characterized by Field Emission Scanning Electron Microscopy (FESEM), fluorescence microscopy, confocal microscopy, and UV–vis spectroscopy. Release of FA from FF Nts were investigated by immersing coated metal substrates in phosphate‐buffered saline for 12 days. Self‐assembly of FF in water and solvent resulted in formation of nanotubes, which efficiently loaded 98% of FA with concentration of 20 µg/mL. FESEM images confirmed successful attachment of FF/FA Nts to functionalized gold substrates. In vitro release studies indicated using FF Nts has prolonged the release rate of FA for several days. Biocompatibility studied confirmed more than 50% of the cells were alive in concentration of 250–1000 µg/mL of FF Nts thus suggesting the potential of peptide based self‐assemble nanostructures as an alternate system for polymer coating in drugs eluting stents. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2280–2290, 2016.
Response of duplex Cr(N)/S and Cr(C)/S coatings on 316L stainless steel to tribocorrosion in 0.89% NaCl solution under plastic contact conditions
Authors: Sun Y. , Dearnley P. A. , Mallia Bertram 
Published: April 2016

AbstractTwo duplex coatings, Cr(N)/S and Cr(C)/S, were deposited on 316 L stainless steel by magnetron sputtering. The effectiveness of these duplex coatings in improving the tribocorrosion behavior of medical alloys under elastic contact conditions has been demonstrated in a recent publication. The present work focused on the response of these duplex coatings to tribocorrosion under plastic contact conditions. Tribocorrosion tests were conducted in 0.89% NaCl solution at 37°C at an initial contact pressure of 740 MPa and under unidirectional sliding conditions for sliding duration up to 24 h. The results showed that during sliding in the corrosive solution, the duplex coatings were plastically deformed into the substrate to a depth about 1 μm. The Cr(C)/S duplex coating had sufficient ductility to accommodate the deformation without cracking, such that it was worn through gradually, leading to the gradual increase in open circuit potential (OCP) and coefficient of friction (COF). On the other hand, the Cr(N)/S duplex coating suffered from cracking at all tested potentials, leading to coating blistering after prolonged sliding at OCP and stable pit formation in the substrate beneath the coating at applied anodic potentials. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1503–1513, 2017.
Enhanced intracellular soluble production of 3‐ketosteroid‐Δ1‐dehydrogenase from Mycobacterium neoaurum in Escherichia coli and its application in the androst‐1,4‐diene‐3,17‐dione production
Authors: Shao Minglong , Chen Yaling , Zhang Xian , Rao Zhiming , Xu Meijuan , Yang Taowei , Li Hui , Xu Zhenghong , Yang Shangtian 
Published: April 2016

AbstractBACKGROUNDThe 3‐ketosteroid‐Δ1‐dehydrogenase (KSDD) expressed in Escherichia coli was mainly in the form of inclusion bodies and the KSDD enzyme activity was at a low level. Therefore, the regulation of process conditions and supplementation of natural osmolytes were carried out to improve the soluble expression of KSDD in E. coli.RESULTSEffects of temperature, inducer concentration, and osmolytes (K‐glutamate, proline, and betaine) supplementation on cell growth and soluble KSDD production in recombinant Escherichia coli were investigated. With the decrease of cultivation temperature, lower isopropyl β‐D‐1‐thiogalactopyranoside (IPTG) concentration, and betaine addition, high intracellular soluble KSDD production was realized in the recombinant E. coli BL21/pET28a‐ksdd and the KSDD enzyme activity reached 11.7 U mg−1, which is the highest KSDD activity ever reported. In addition, the whole‐cell biocatalyst of this recombinant strain was used for bioconversion of androst‐4‐ene‐3,17‐dione (AD) to androst‐1,4‐diene‐3,17‐dione (ADD). By optimization of the transformation conditions and applying a fed‐batch strategy, a final ADD yield of 5.7 g L−1 was achieved with a productivity of 0.158 g (L h)−1, which is the highest reported productivity using a microbial process. More importantly, no by‐products were detected during the whole bioconversion process.CONCLUSIONThese results provide the basis for industrial scale production of ADD. © 2016 Society of Chemical Industry
The effects of weight loss after sleeve gastrectomy on left ventricular systolic function in men versus women
Authors: Kemaloğlu Öz Tuğba , Ünal Dayı Şennur , Seyit Hakan , Öz Ayhan , Ösken Altuğ , Atasoy Işıl , Yıldız Ufuk , Özpamuk Karadeniz Fatma , İpek Göktürk , Köneş Osman , Alış Halil 
Published: April 2016

ABSTRACTPurposeTo evaluate and compare the effects of weight lost after sleeve gastrectomy on left ventricular (LV) systolic function using both two‐dimensional speckle tracking (2D‐STE) and three‐dimensional echocardiography (3DE) in men versus women.MethodsIn 53 obese patients referred for sleeve gastrectomy, 2D‐STE and 3DE were performed prior to and 6 months after surgery.ResultsThe study included 53 obese patients (62.3% female; mean age 36.8 ± 10.7 years). Six months after surgery, all patients demonstrated a significant decrease in body mass index, body weight, blood pressure, heart rate, LV end‐diastolic dimension, myocardial wall thickness, LV mass, LV mass index, LV mass/height2.7, LV end‐diastolic volume, LV end‐systolic volume, and stroke volume as well as an increase in SV index and ejection fraction. There was no significant difference in measured variables between men and women at baseline or postsurgery, except for baseline LV end‐diastolic dimension, and baseline and after surgery LV mass, LV mass index, and LV mass/height2.7, which were all significantly higher in men.ConclusionsSleeve gastrectomy improves LV systolic function and contributes to reverse LV remodeling in both genders. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 44:492–499, 2016
Percutaneous ultrasound‐guided core needle biopsy of solid pancreatic masses: Results in 250 patients
Authors: Kahriman Guven , Ozcan Nevzat , Dogan Serap , Ozmen Soner , Deniz Kemal 
Published: April 2016

ABSTRACTBackgroundTo determine the diagnostic accuracy and complications of percutaneous sonographic (US)‐guided core needle‐needle biopsy in the diagnosis of solid pancreatic masses.MethodsCases of US‐guided percutaneous core needle biopsy of solid pancreatic masses performed in our department between July 2009 and June 2015 were analyzed retrospectively. The demographic data, lesions' size and location, pathology results, accuracy, sensitivity, specificity, positive predictive value, negative predictive value, and complications of the biopsies were determined.ResultsA total of 250 patients (150 males, 100 females; age range, 16–88 years; mean age, 64.3 ± 12.1 years) were included in the study. The overall diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of all 250 biopsies were 94.8%, 94.3%, 97.2%, 99.5%, and 75%, respectively, and changed to 98.4%, 99%, 94.7%, 99%, and 94.7%, respectively, after the biopsy was repeated in 12 patients. Four (1.6%) major complications, including a pseudoaneurysm of the gastroduodenal artery, and three cases of acute pancreatitis, and one (0.4%) minor complication (a vaso‐vagal syncope), were observed. There was no biopsy‐related death.ConclusionsUS‐guided percutaneous core needle biopsy is a safe and highly effective method with acceptable complication rates in the diagnosis of solid pancreatic masses. © 2016 Wiley Periodicals, Inc. J Clin Ultrasound 44:470–473, 2016
Successful implementation of outpatient parenteral antimicrobial therapy at a medical respite facility for homeless patients
Authors: Beieler Alison M. , Dellit Timothy H. , Chan Jeannie D. , Dhanireddy Shireesha , Enzian Leslie K. , Stone Tamera J. , Dwyer‐O'Connor Edward , Lynch John B. 
Published: April 2016

BACKGROUNDOutpatient parenteral antimicrobial therapy (OPAT) is a safe way to administer intravenous (IV) antimicrobial therapy to patients with the potential to decrease hospital length of stay (LOS). Often, homeless patients with complex infections, who could otherwise be treated as an outpatient, remain in the hospital for the duration of IV antibiotic treatment. Injection drug use (IDU) is a barrier to OPAT.OBJECTIVETo evaluate our experience with administering OPAT to homeless patients at a medical respite facility and determine if patients could complete a successful course of antibiotics.DESIGNUsing retrospective chart review, demographics, diagnosis, and comorbidities including mental illness, current IDU, and remote IDU (>3 months ago) were recorded. Surgical, microbiologic, and antimicrobial therapy including route (IV or oral), duration of therapy, and adverse events were abstracted.PARTICIPANTSHomeless patients >18 years old who received OPAT at medical respite after discharge, no exclusions.MAIN MEASUREMENTSPrimary outcome was successful completion of OPAT at medical respite. Secondary outcome was successful antimicrobial course completion for a specific diagnosis.RESULTSForty‐six (87%) patients successfully completed a defined course of antibiotic therapy. Thirty‐four (64%) patients were successfully treated with OPAT at medical respite. Readmission rate was 30%. The average length of OPAT was 22 days. The cost savings to our institution (using $1500/day inpatient cost) was $25,000 per episode of OPAT.CONCLUSIONSOPAT can be successful in a supervised medical respite setting for homeless patients with the help of a multidisciplinary team, and can decrease inpatient LOS resulting in cost savings. Journal of Hospital Medicine 2016;11:531–535. © 2016 Society of Hospital Medicine
Magnetic solid‐phase extraction for determination of the total malachite green, gentian violet and leucomalachite green, leucogentian violet in aquaculture water by high‐performance liquid chromatography with fluorescence detection
Authors: Zhao Jiao , Wei Daqiao , Yang Yaling 
Published: April 2016

In this study, magnetic multi‐walled carbon nanotube nanoparticles were synthesized and used as the adsorbent for the sums of malachite green, gentian violet and leucomalachite green, leucogentian violet in aquaculture water samples followed by high performance liquid chromatography with fluorescence detection. This method was based on in situ reduction of chromic malachite green, gentian violet to colorless leucomalachite green, leucogentian violet with potassium borohydride, respectively. The obtained adsorbent combines the advantages of carbon nanotubes and Fe3O4 nanoparticles in one material for separation and preconcentration of the reductive dyes in aqueous media. The structure and properties of the prepared nanoparticles were characterized by transmission and scanning electron microscopy, X‐ray diffraction, and Fourier‐transform infrared spectroscopy. The main parameters affecting the adsorption recoveries were investigated and optimized, including reducing agent concentration, type and amount of sorbent, sample pH, and eluting conditions. Under the optimum conditions, the limits of detection in this method were 0.22 and 0.09 ng/mL for malachite green and gentian violet, respectively. Product recoveries ranged from 87.0 to 92.8% with relative standard deviations from 4.6 to 5.9%. The results indicate that the sorbent is a suitable material for the removal and concentration of triphenylmethane dyes from polluted environmental samples.
Office‐based biopsies for laryngeal lesions: Analysis of consecutive 581 cases
Authors: Cha Wonjae , Yoon Byung‐Woo , Jang Jeon Yeob , Lee Jin Choon , Lee Byung Joo , Wang Soo‐Geun , Cho Jae Keun , Cho Ilyoung 
Published: April 2016

Objectives/HypothesisOffice‐based biopsy (OBB) is widely used to diagnose suspicious laryngeal lesions. However, its routine use is still controversial and the algorithm for clinical strategy is not concrete. We tried to evaluate the accuracy of OBB as a diagnostic tool for laryngeal malignancy.MethodsMedical data of 581 patients undergoing OBB were reviewed. Diagnostic values of OBB were analyzed for malignancy and malignancy/premalignancy. False negative rates (FNRs) were analyzed according to subsites, lesion sizes, and morphology.ResultsThe collection rate of adequate specimens was 99.1%, and there were no complications during OBB. The false omission rates for malignancy of benign lesion; mild, moderate, and severe dysplasia; and carcinoma in situ (CIS) were 5.1%, 11.9%, 14.3%, 50.0%, and 100.0%, respectively. Sensitivity, specificity, positive predictive value, and negative predictive value for malignancy in OBB were 78.2%, 100.0%, 100.0%, and 87.3%, respectively. Those for malignancy/premalignancy in OBB were 88.2%, 86.7%, 85.7%, and 89.1%, respectively. The high FNRs were reported in glottis lesions (27.0%) and small lesions below 1 cm (27.5%). Morphologically fungating lesions showed low FNR (9.1%) compared to nonfungating ones.ConclusionOffice‐based biopsy could be used as an initial diagnostic tool for laryngeal malignancy for early detection and avoidance of general anesthesia. However, operative re‐biopsy should be considered when severe dysplasia or CIS were reported in OBB, or when the lesions are clinically suspicious for malignancy.Level of Evidence4. Laryngoscope, 126:2513–2519, 2016
The presentation and management of granulomatosis with polyangiitis (Wegener's Granulomatosis) in the pediatric airway
Authors: Lee Pui Y. , Adil Eelam A. , Irace Alexandria L. , Neff Laura , Son Mary Beth F. , Lee Edward Y. , Perez‐Atayde Antonio , Rahbar Reza 
Published: April 2016

ObjectiveGranulomatosis with polyangiitis (GPA) is a necrotizing inflammatory disease that can affect the airway. The purpose of this study was to present a case of pediatric laryngotracheal GPA and provide management recommendations based on a thorough review of the literature.Data SourcesRetrospective chart and literature reviewReview MethodsA pediatric patient with laryngotracheal and pulmonary manifestations of GPA who underwent chemotherapy and intralesional corticosteroid injection is described. An extensive literature review of pediatric GPA affecting the larynx/trachea was also performed.ResultsA pediatric patient presented with acute respiratory distress. Flexible laryngoscopy revealed a laryngeal mass. Magnetic resonance imaging showed circumferential subglottic stenosis, and chest computed tomography demonstrated multiple pulmonary nodules. Laryngeal and tracheal biopsy revealed granulation tissue and primary vasculitis. Labs demonstrated positive cytoplasmic antineutrophil cytoplasmic antibody, consistent with GPA. Methylprednisone, rituximab, cyclophosphamide, and intralesional steroid injection resulted in remission after 12 weeks. Review of the literature revealed two pediatric cases series and 10 case reports of GPA affecting the larynx or trachea.ConclusionsThere is a higher prevalence of GPA of the airway in children when compared to adults. Biopsy of the airway lesion may not be necessary and has lower diagnostic yield compared to other GPA subsites. Medical management includes induction therapy followed by maintenance therapy once the disease is in remission. Most patients will require a surgical intervention to maintain the airway.Level of EvidenceNA Laryngoscope, 127:233–240, 2017
Cerebral metabolic rate of oxygen (CMRO2) mapping with hyperventilation challenge using quantitative susceptibility mapping (QSM)
Authors: Zhang Jingwei , Zhou Dong , Nguyen Thanh D. , Spincemaille Pascal , Gupta Ajay , Wang Yi 
Published: April 2016

PurposeOur objective was to demonstrate the feasibility of using hyperventilation as an efficient vasoconstrictive challenge and prior knowledge as denoising constraints for cerebral metabolic rate of oxygen (CMRO2) mapping based upon quantitative susceptibility mapping (QSM).MethodsThree‐dimensional (3D) multi‐echo gradient echo and arterial spin labeling imaging were performed to calculate QSM and perfusion maps before and after a hyperventilation challenge in 11 healthy subjects. For comparison, this was repeated using a caffeine challenge. Whole‐brain CMRO2 and oxygen extraction fraction (OEF) maps were computed using constrained optimization. Hyperventilation scans were repeated to measure reproducibility. Regional agreement of CMRO2 and OEF maps was analyzed within the cortical gray matter (CGM) using t‐test and Bland‐Altman plots.ResultsHyperventilation challenge eliminates the 30‐min waiting time needed for caffeine to exert its vasoconstrictive effects. Mean CMRO2 (in µmol/100g/min) obtained in CGM using the caffeine and repeated hyperventilation scans were 149 ± 16, 153 ± 19, and 150 ± 20, respectively. This corresponded to an OEF of 33.6 ± 3.4%, 32.3 ± 3.2%, and 34.1 ± 3.8% at baseline state and 39.8 ± 4.8%, 43.6 ± 6.2%, and 42.8 ± 6.8% at challenged state, respectively. Hyperventilation scans produced a good agreement of CMRO2 and OEF values.ConclusionsHyperventilation is a feasible, reproducible, and efficient vasoconstrictive challenge for QSM‐based quantitative CMRO2 mapping. Magn Reson Med 77:1762–1773, 2017. © 2016 International Society for Magnetic Resonance in Medicine
Rasch analysis of the Pediatric Evaluation of Disability Inventory–computer adaptive test (PEDI‐CAT) item bank for children and young adults with spinal muscular atrophy
Authors: Pasternak Amy , Sideridis Georgios , Fragala‐Pinkham Maria , Glanzman Allan M. , Montes Jacqueline , Dunaway Sally , Salazar Rachel , Quigley Janet , Pandya Shree , O'Riley Susan , Greenwood Jonathan , Chiriboga Claudia , Finkel Richard , Tennekoon Gihan , Martens William B. , McDermott Michael P. , Fournier Heather (Szelag) , Madabusi Lavanya , Harrington Timothy , Cruz Rosangel E. , LaMarca Nicole M. , Videon Nancy M. , Vivo Darryl C. De , Darras Basil T. ,   the Muscle Study Group (MSG) and the Pediatric Neuromuscular Clinical Research Network for Spinal Muscular Atrophy (PNCRN)
Published: April 2016

ABSTRACTIntroduction: In this study we evaluated the suitability of a caregiver‐reported functional measure, the Pediatric Evaluation of Disability Inventory–Computer Adaptive Test (PEDI‐CAT), for children and young adults with spinal muscular atrophy (SMA). Methods: PEDI‐CAT Mobility and Daily Activities domain item banks were administered to 58 caregivers of children and young adults with SMA. Rasch analysis was used to evaluate test properties across SMA types. Results: Unidimensional content for each domain was confirmed. The PEDI‐CAT was most informative for type III SMA, with ability levels distributed close to 0.0 logits in both domains. It was less informative for types I and II SMA, especially for mobility skills. Item and person abilities were not distributed evenly across all types. Conclusions: The PEDI‐CAT may be used to measure functional performance in SMA, but additional items are needed to identify small changes in function and best represent the abilities of all types of SMA. Muscle Nerve 54: 1097–1107, 2016