bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain
Authors: Wen Yurong , Ouyang Zhenlin , Devreese Bart , He Wangxiao , Shao Yongping , Lu Wuyuan , Zheng Fang 
Published: July 2017

AbstractThe master regulator CsgD switches planktonic growth to biofilm formation by activating synthesis of curli fimbriae and cellulose in Enterobacteriaceae. CsgD was classified to be the LuxR response regulatory family, while its cognate sensor histidine kinase has not been identified yet. CsgD consists of a C‐terminal DNA binding domain and an N‐terminal regulatory domain that provokes the upstream signal transduction to further modulate its function. We provide the crystal structure of Salmonella Typhimurium CsgD regulatory domain, which reveals an atypical β5α5 response regulatory receiver domain folding with the α2 helix representing as a disorder loop compared to the LuxR/FixJ canonical response regulator, and the structure indicated a noteworthy α5 helix similar to the non‐canonical master regulator VpsT receiver domain α6. CsgD regulatory domain assembles with two dimerization interfaces mainly through α1 and α5, which has shown similarity to the c‐di‐GMP independent and stabilized dimerization interface of VpsT from Vibrio cholerae respectively. The potential phosphorylation site D59 is directly involved in the interaction of interfaces I and mutagenesis studies indicated that both dimerization interfaces could be crucial for CsgD activity. The structure reveals important molecular details for the dimerization assembly of CsgD and will shed new insight into its regulation mechanism.
Structural and SAXS analysis of Tle5–Tli5 complex reveals a novel inhibition mechanism of H2‐T6SS in Pseudomonas aeruginosa
Authors: Yang Xiao‐Yun , Li Zong‐Qiang , Gao Zeng‐Qiang , Wang Wen‐Jia , Geng Zhi , Xu Jian‐Hua , She Zhun , Dong Yu‐Hui 
Published: July 2017

AbstractWidely spread in Gram‐negative bacteria, the type VI secretion system (T6SS) secretes many effector‐immunity protein pairs to help the bacteria compete against other prokaryotic rivals, and infect their eukaryotic hosts. Tle5 and Tle5B are two phospholipase effector protein secreted by T6SS of Pseudomonas aeruginosa. They can facilitate the bacterial internalization process into human epithelial cells by interacting with Akt protein of the PI3K‐Akt signal pathway. Tli5 and PA5086‐5088 are cognate immunity proteins of Tle5 and Tle5B, respectively. They can interact with their cognate effector proteins to suppress their virulence. Here, we report the crystal structure of Tli5 at 2.8Å resolution and successfully fit it into the Small angle X‐ray scattering (SAXS) model of the complete Tle5–Tli5 complex. We identified two important motifs in Tli5 through sequence and structural analysis. One is a conserved loop‐β‐hairpin motif that exists in the Tle5 immunity homologs, the other is a long and sharp α‐α motif that directly interacts with Tle5 according to SAXS data. We also distinguished the structural features of Tle5 and Tle5B family immunity proteins. Together, our work provided insights into a novel inhibition mechanism that may enhance our understanding of phospholipase D family proteins.
Theoretical study on the interactions of halogen‐bonds and pnicogen‐bonds in phosphine derivatives with Br2, BrCl, and BrF
Authors: Jiao Yinchun , Liu Yi , Zhao Wenjing , Wang Zhaoxu , Ding Xunlei , Liu Hexiu , Lu Tian 
Published: July 2017

AbstractThe MP2 ab initio quantum chemistry methods were utilized to study the halogen‐bond and pnicogen‐bond system formed between PH2X (X = Br, CH3, OH, CN, NO2, CF3) and BrY (Y = Br, Cl, F). Calculated results show that all substituent can form halogen‐bond complexes while part substituent can form pnicogen‐bond complexes. Traditional, chlorine‐shared and ion‐pair halogen‐bonds complexes have been found with the different substituent X and Y. The halogen‐bonds are stronger than the related pnicogen‐bonds. For halogen‐bonds, strongly electronegative substituents which are connected to the Lewis acid can strengthen the bonds and significantly influenced the structures and properties of the compounds. In contrast, the substituents which connected to the Lewis bases can produce opposite effects. The interaction energies of halogen‐bonds are 2.56 to 32.06 kcal·mol−1; The strongest halogen‐bond was found in the complex of PH2OH•••BrF. The interaction energies of pnicogen‐bonds are in the range 1.20 to 2.28 kcal·mol−1; the strongest pnicogen‐bond was found in PH2Br•••Br2 complex. The charge transfer of lp(P) σ*(BrY), lp(F) σ*(BrP), and lp(Br) σ*(XP) play important roles in the formation of the halogen‐bonds and pnicogen‐bonds, which lead to polarization of the monomers. The polarization caused by the halogen‐bond is more obvious than that by the pnicogen‐bond, resulting in that some halogen‐bonds having little covalent character. The symmetry adapted perturbation theory (SAPT) energy decomposition analysis showes that the halogen‐bond and pnicogen‐bond interactions are predominantly electrostatic and dispersion, respectively.
Inhibition of Protein Synthesis with Highly Soluble Caged Compounds
Authors: Marter Kathrin , Wetzel Janina , Eichhorst Jenny , Eremina Nadja , Leboulle Gérard , Barth Andreas , Wiesner Burkhard , Eisenhardt Dorothea 
Published: July 2017

AbstractTo target protein synthesis in defined areas, e. g. neuropiles of small brains or subcellular structures, locally restricted inhibition of protein synthesis is needed and can be realized by caged compounds of protein synthesis inhibitors (PSI). Since organic solvents interfere with protein synthesis themselves, the use of water‐soluble caged PSIs is a prerequisite in studies on protein synthesis. Such compounds are sparsely available. We developed and characterized efficient highly soluble caged compounds of the PSIs anisomycin and emetine masking their biological activity with a {8‐[bis(carboxymethyl)aminomethyl]‐6‐bromo‐7‐hydroxycoumarin‐4‐yl}methoxycarbonyl (BBHCMOC) derivative. The absorption spectra of the resulting BBHCMOC‐caged anisomycin and BBHCMOC‐caged emetine show long‐wavelength maxima and the extinction coefficients are high, allowing uncaging under non‐damaging light conditions. When uncaged, these caged PSIs reliably inhibit protein synthesis in an in vitro translation system and in cell culture. Taken the whole spectrum of properties into account, our BBHCMOC‐caged PSIs are highly qualified for in vivo studies.
Disentangling the effects of predation and oceanographic fluctuations in the mortality of two allopatric seabird populations
Authors: Matović Nikola , Cadiou Bernard , Oro Daniel , Sanz‐Aguilar Ana 
Published: July 2017

AbstractLife‐history traits of migratory seabirds are influenced by changing conditions at breeding and wintering grounds. Climatic conditions and predation are known to impact populations’ survival rates, but few studies examine their effect simultaneously. We used multievent capture–recapture models to assess mortality due to environmental conditions and predation in breeding European storm petrels (Hydrobates pelagicus) in two allopatric colonies (Mediterranean and Atlantic). Predatory mortality at the colonies showed annual variation, being around 0.05 in certain years. Mortality at sea differed between the two oceanic basins, and was lower in the Mediterranean colony [0.11, 95% CI (0.09, 0.14)] when compared to the Atlantic colony [0.18, 95% CI (0.15, 0.22)]. The Western Mediterranean Oscillation index (WeMOi) explained 57% of the temporal variability in mortality of Mediterranean breeders. In comparison, 43% of the temporal variability in mortality of Atlantic breeders was explained by the winter St Helena index (wHIX) and El Niño‐Southern Oscillation index (wENSO). Our results suggest that Mediterranean breeders remain in this basin for wintering where they may face lower migratory costs and more favourable environmental conditions. In contrast, Atlantic breeders’ mortality may be due to higher cost of migration, changing upwelling conditions in the Benguela current and heavy storms over their migratory route during La Niña events. This study underlines the importance of modelling separately different causes of mortality when testing the effects of climatic covariates.
Differential Scanning Calorimetry (DSC) and Thermodynamic Prediction of Liquid Fraction vs Temperature for Two High-Performance Alloys for Semi-Solid Processing (Al-Si-Cu-Mg (319s) and Al-Cu-Ag (201))
Authors: Zhang Duyao , Atkinson Helen V. , Dong Hongbiao , Zhu Qiang 
Published: July 2017

Abstract
There is a need to extend the application of semi-solid processing (SSP) to higher performance alloys such as 319s (Al-Si-Cu-Mg) and 201 (Al-Cu-Ag). The melting of these two alloys was investigated using differential scanning calorimetry (DSC) and thermodynamic prediction. The alloys had been processed by magneto-hydrodynamic (MHD) stirring before receipt to produce a microstructure suitable for SSP. The DSC results for the as-received MHD material were compared with those for material which has been taken through a complete DSC cycle and then reheated for a second DSC run. The effects of microsegregation were then analyzed. A higher liquid fraction for a particular temperature is found in the second DSC run than the first. Microstructural observations suggest this is because the intermetallics which form during the first cooling cycle tend to co-located. Quaternary and ternary reactions then occur during the second DSC heat and the co-location leads to enhanced peaks. The calculated liquid fraction is lower with 10 K/min DSC heating rate comparing with 3 K/min at a given temperature. The DSC scan rate must therefore be carefully considered if it is to be used to identify temperature parameters or the suitability of alloys for SSP. In addition, the starting material for DSC must represent the starting material for the SSP. With thermodynamic prediction, the equilibrium condition will provide better guidance for the thixoforming of MHD stirred starting material than the Scheil condition. The Scheil mode approximates more closely with a strongly microsegregated state.
Phospholipid Profiles of Oleaginous Pressed Cakes Using NMR and Gas Chromatography
Authors: Cansell Maud , Bardeau Tiphaine , Morvan Estelle , Grélard Axelle , Buré Corinne , Subra‐Paternault Pascale 
Published: July 2017

AbstractCamelina, flaxseed, hemp, sesame, and walnut cakes were analyzed for their phospholipid (PL) content and composition using 31P and 1H nuclear magnetic resonance and gas chromatography. The data evidenced variations between the sources in terms of (1) total lipid content and PL concentration, camelina cake being the richest source of PLs, (2) PL composition, phosphatidylcholine being the most abundant phospholipid in sesame and hemp cakes, whereas phosphatidylinositol represented about 25% of the total PLs in most cakes, and (3) fatty acid composition of the PLs, camelina cake being the richest source of omega 3 polyunsaturated fatty acids. These data may be useful to diversify the PL sources available and to provide PL fractions with specific nutritional or functional properties.
Enzymatic Production of Highly Unsaturated Monoacyglycerols and Diacylglycerols and Their Emulsifying Effects on the Storage Stability of a Palm Oil Based Shortening System
Authors: Zhang Zhen , Ma Xiang , Huang Huihua , Li Guanghui , Wang Yong 
Published: July 2017

AbstractMDs [monoacylglycerols (MAGs) and diacylglycerols (DAGs) mixture] are widely‐used emulsifiers in specialty fats industrial production. An enzymatic production of highly unsaturated MDs (HUSMDs) and its effects on the storage stability of a palm oil‐based shortening system are reported. Oleic acid and corn oil were used to produce HUSMDs in a bubble column reactor (BCR) system in the presence of Novozyme 435. Under the optimized reaction conditions, the content of HUSMDs in the products was above 82 wt% with 46.67 wt% of MAGs and 35.56 wt% DAGs, respectively. Moreover, in the subsequent evaluation of MDs’ effects on the storage stabilities of a palm oil‐based shortening system (IEPO), HUSMDs proved to be a potent emulsifier with decent aeration properties and a possible alternative to saturated MAGs and DAGs (SMDs) made from fully hydrogenated high erucic acid colza oil. Compared with SMDs, HUSMDs decreased the crystallization rate significantly. The microstructure of them shows improved stability of β′ crystals, and no obvious aggregation of crystals was recorded in IEPO with HUSMDs, which also demonstrated the most stable hardness.
BEEF CATTLE RETAINED OWNERSHIP AND PROFITABILITY IN TENNESSEE
Authors: TANG MINFENG , LEWIS KAREN E. , LAMBERT DAYTON M. , GRIFFITH ANDREW P. , BOYER CHRISTOPHER N. 
Published: July 2017

AbstractThe effect of animal characteristics and placement decisions on retained ownership profitability of Tennessee cattle from 2005 to 2015 was determined using a mixed model regression. Ex post simulation analysis examined retained ownership profitability by placement season under different animal characteristic and corn price scenarios. Regression results indicate that placement weight, placement season, days on feed, animal health, and animal sex affect retained ownership profitability. Simulation results indicate that winter placement of cattle in feedlots had the highest expected retained ownership profits. Results provide risk-averse producers information regarding the profitability of retained ownership.
Rescue extracorporeal membrane oxygenation therapy in methamphetamine toxicity
Authors: Morrison Laura K. , Kromm J , Gaudet J , Zuege D , Button B , Warshawski F , Lucyk SN 
Published: July 2017

AbstractComplications related to methamphetamine use and abuse are common presentations seen in the emergency department. Standard management focuses on addressing the central nervous system and cardiovascular effects with the use of sedation and hemodynamic support. We describe a case report of a patient with methamphetamine toxicity and subsequent severe cardiomyopathy refractory to conventional management that responded to cardiovascular support with extracorporeal membrane oxygenation therapy (ECMO). A 22-year-old female was admitted in severe cardiogenic shock following intravenous administration of methamphetamine and oral fentanyl use. Despite aggressive treatment with benzodiazepines, intravenous fluids, vasopressors, vasodilators, antibiotics and inotropes, the patient’s clinical status deteriorated, and she suffered a cardiac arrest. The patient was successfully resuscitated, and following the return of spontaneous circulation, ECMO was initiated. After 82 hours the patient was successfully weaned from ECMO with the recovery of her left ventricular function and no neurologic sequelae. The patient developed leg ischemia requiring embolectomy and open repair as a complication of ECMO cannulation. In our case, ECMO was used successfully in treating severe cardiac dysfunction from acute methamphetamine-induced cardiomyopathy and was used as a bridge to recovery. The complications seen in this patient emphasize the potential risks associated with this intervention and highlight the need for careful patient selection.
The Ongoing Syrian Arab Republic Health Care Crisis
Authors: Phillips Steven J. 
Published: July 2017

AbstractPrior to the Syrian civil war, access and delivery of health care and health care information over the past 4 decades had steadily improved. The life expectancy of the average Syrian in 2012 was 75.7 years, compared to 56 years in 1970. As a result of the civil war, this trend has reversed, with the life expectancy reduced by 20 years from the 2012 level. The Syrian government and its allies have specifically targeted the health care infrastructure not under government control. (Disaster Med Public Health Preparedness. 2018;12:23–25)
Variable Case Detection and Many Unreported Cases of Surgical-Site Infection Following Colon Surgery and Abdominal Hysterectomy in a Statewide Validation
Authors: Calderwood Michael S. , Huang Susan S. , Keller Vicki , Bruce Christina B. , Kazerouni N. Neely , Janssen Lynn 
Published: July 2017

OBJECTIVETo assess hospital surgical-site infection (SSI) identification and reporting following colon surgery and abdominal hysterectomy via a statewide external validationMETHODSInfection preventionists (IPs) from the California Department of Public Health (CDPH) performed on-site SSI validation for surgical procedures performed in hospitals that voluntarily participated. Validation involved chart review of SSI cases previously reported by hospitals plus review of patient records flagged for review by claims codes suggestive of SSI. We assessed the sensitivity of traditional surveillance and the added benefit of claims-based surveillance. We also evaluated the positive predictive value of claims-based surveillance (ie, workload efficiency).RESULTSUpon validation review, CDPH IPs identified 239 SSIs following colon surgery at 42 hospitals and 76 SSIs following abdominal hysterectomy at 34 hospitals. For colon surgery, traditional surveillance had a sensitivity of 50% (47% for deep incisional or organ/space [DI/OS] SSI), compared to 84% (88% for DI/OS SSI) for claims-based surveillance. For abdominal hysterectomy, traditional surveillance had a sensitivity of 68% (67% for DI/OS SSI) compared to 74% (78% for DI/OS SSI) for claims-based surveillance. Claims-based surveillance was also efficient, with 1 SSI identified for every 2 patients flagged for review who had undergone abdominal hysterectomy and for every 2.6 patients flagged for review who had undergone colon surgery. Overall, CDPH identified previously unreported SSIs in 74% of validation hospitals performing colon surgery and 35% of validation hospitals performing abdominal hysterectomy.CONCLUSIONSClaims-based surveillance is a standardized approach that hospitals can use to augment traditional surveillance methods and health departments can use for external validation.Infect Control Hosp Epidemiol 2017;38:1091–1097
Antibiotic Overuse is a Major Risk Factor for Clostridium difficile Infection in Surgical Patients
Authors: Bernatz James T. , Safdar Nasia , Hetzel Scott , Anderson Paul A. 
Published: July 2017

Clostridium difficile infection (CDI) is associated with increased cost, morbidity, and mortality in postoperative patients. Variable rates of postoperative CDI are reported among 4 surgical specialties during the 30-month study period. Risk factors for CDI include antibiotic use, increased ASA score, and increased admissions in the past year.Infect Control Hosp Epidemiol 2017;38:1254–1257
FROM SUZHOU TO SHANGHAI: A TALE OF TWO SYSTEMS
Authors: Marmé Michael 
Published: July 2017

AbstractScholars have long assumed that the opening of Shanghai as a treaty port in 1843, followed by the disruption caused by the Taiping Rebellion, led to an abrupt restructuring of China's internal organization and a fundamental change in its relation to the outside world. Looking at developments at Suzhou and Shanghai over the long nineteenth century in parallel, this study argues that this was in fact a far later and much more gradual process than we have heretofore appreciated, the decisive breaks occurring at least a half-century later than usually assumed.
Crassicauda boopis in a fin whale (Balaenoptera physalus) ship-struck in the eastern North Atlantic Ocean
Authors: LEMPEREUR LAETITIA , DELOBELLE MORGAN , DOOM MARJAN , HAELTERS JAN , LEVY ETIENNE , LOSSON BERTRAND , JAUNIAUX THIERRY 
Published: July 2017

SUMMARY
On 9 November 2015, a juvenile male fin whale of 11·60 m length was observed on the bulb of a merchant vessel in the Channel Terneuzen – Ghent (The Netherlands – Belgium). A severe parasitosis was present in the right heart ventricle and caudal caval vein. Parasites were identified as Crassicauda boopis based on macroscopic and microscopic observations. The sequence of the 18S rRNA gene obtained from the parasite samples was 100% similar to the sequence of the 18S rRNA gene from Crassicauda magna available on GenBank. While adults of C. boopis and C. magna are morphologically distinct and found at different locations in the body, the molecular analysis of the 18S rRNA gene seems insufficient for reliable species identification. Although numerous C. boopis were found, the cause of death was identified as due to the collision with the ship, as suggested by the presence of a large haematoma, and the absence of evidence of renal failure. The young age of this whale and the absence of severe chronic reaction may suggest that the infestation was not yet at an advanced chronic stage.
Vitamin D deficiency, oxidative stress and antioxidant status: only weak association seen in the absence of advanced age, obesity or pre-existing disease
Authors: Wang Erica W. , Siu Parco M. , Pang Marco Y. , Woo Jean , Collins Andrew R. , Benzie Iris F. F. 
Published: July 2017

AbstractVitamin D deficiency (plasma 25-hydroxycholecalciferol (25(OH)D)<50 nmol/l) is highly prevalent, increases risk of non-communicable diseases (NCD) and associates with increased oxidative stress in obese subjects, the elderly and patients suffering from NCD. If confirmed as an independent driver of oxidative stress, nutritional and other public health strategies to improve vitamin D status would be strongly supported. We investigated vitamin D/oxidative stress links without the confounding effects of advanced age, obesity, smoking or pre-existing disease. Plasma 25(OH)D and biomarkers of oxidative stress and antioxidant status (plasma allantoin, oxidised LDL, ferric reducing antioxidant power (FRAP), ascorbic acid, urine 8-oxo-7,8-dihydro-2'-deoxyguanosine) were measured in fasting samples from 196 consenting, healthy adults aged 18–26 years. Correlation between 25(OH)D and each biomarker as well as biomarker differences across 25(OH)D quartiles and groups (<25/25–49/≥50 nmol/l) were investigated. Median 25(OH)D was 40 nmol/l; >70 % of participants were vitamin D deficient. No significant correlations and no biomarker differences across 25(OH)D quartiles or groups were seen except for total antioxidant status. A weak direct association (r0·252,P<0·05) was observed between 25(OH)D and FRAP, and those in the lowest 25(OH)D quartile and group had significantly lower FRAP values. Results did not reveal a clear link between vitamin D status and oxidative stress biomarkers in the absence of advanced age, obesity and disease, though some evidence of depleted antioxidant status in those with vitamin D deficiency was seen. Poor antioxidant status may pre-date increased oxidative stress. Study of effects of correction of deficiency on antioxidant status and oxidative stress in vitamin D-deficient but otherwise healthy subjects is needed.