bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet activation by Shiga toxin and circulatory factors as a pathogenetic mechanism in the hemolytic uremic syndrome
Authors: Karpman Diana , Papadopoulou Domniki , Nilsson Kajsa , Sjögren Ann-Christine , Mikaelsson Carl , Lethagen Stefan 
Published: July 2002

AbstractThrombocytopenia caused by platelet consumption in thrombi is a major manifestation of hemolytic uremic syndrome (HUS) associated with Shiga toxin (Stx) producing Escherichia coli. Platelets have glycosphingolipid receptors capable of binding Stx, but a direct interaction between the toxin and platelets, leading to platelet activation, has not been reported. In this study, it is shown that Stx1 and its B (binding) subunit (Stx1B), at 10 pg/mL to 10 ng/mL, bound to platelets. Toxin was internalized in platelets within 2 hours. This led to increased platelet aggregation, as demonstrated by confocal microscopy. Preincubation of Stx1B with anti-Stx1 antibody inhibited this reaction. Stx1 induced morphologic changes in platelets seen on scanning electron microscopy. In the presence of platelets and tumor necrosis factor–pretreated human umbilical vein endothelial cells (HUVEC), Stx1 and Stx1B induced the binding of platelets to the endothelial cell membrane and were present at this binding site. Incubation of Stx1 and Stx1B with whole blood increased fibrinogen binding to platelets detected by flow cytometry. Fibrinogen binding was partially inhibited by preincubation with anti-Stx1. Stx1 increased platelet retention measured in a glass bead assay. In addition, plasma from 17 patients with HUS, taken during the acute phase of the disease, increased the retention of normal platelets and normalized after recovery. Taken together, the results of this investigation show that Stx1, Stx1B, and a factor or factors in the plasma of patients with HUS activate platelets. The presence of Stx1 at the binding site of platelets to HUVEC suggests that Stx may be directly involved in the prothrombotic state seen in HUS.
Integrin-associated protein is an adhesion receptor on sickle red blood cells for immobilized thrombospondin
Authors: Brittain Julia E. , Mlinar Kathryn J. , Anderson Christopher S. , Orringer Eugene P. , Parise Leslie V. 
Published: July 2002

AbstractThe adhesive protein thrombospondin (TSP) potentially mediates sickle (SS) red blood cell (RBC) adhesion to the blood vessel wall, thereby contributing to vaso-occlusive crises in sickle cell disease. We previously reported that SS RBCs bind to immobilized TSP under flow conditions, whereas normal (AA) red cells do not. However, the SS RBC receptors that mediate this interaction are largely unknown. Here it is reported that integrin-associated protein (IAP), or CD47, mediates the adhesion of these cells to immobilized TSP under both flow and static conditions. A peptide derived from the C-terminal IAP binding site of TSP also supports sickle cell adhesion; adhesion to this peptide or to TSP is inhibited specifically by the anti-IAP monoclonal antibody, 1F7. Furthermore, these data suggest that IAP on SS RBCs is structurally different from that expressed on AA RBCs but that IAP expression levels do not vary between AA and SS RBCs. This structural difference may contribute to the enhanced adhesion of SS RBCs to immobilized TSP. These results identify IAP as a TSP receptor on SS RBCs and suggest that this receptor and its binding site within TSP represent potential therapeutic targets to decrease vaso-occlusion.
Increased granulopoiesis through interleukin-17 and granulocyte colony-stimulating factor in leukocyte adhesion molecule–deficient mice
Authors: Forlow S. Bradley , Schurr Jill R. , Kolls Jay K. , Bagby Gregory J. , Schwarzenberger Paul O. , Ley Klaus 
Published: July 2002

AbstractMany mutant mice deficient in leukocyte adhesion molecules display altered hematopoiesis and neutrophilia. This study investigated whether peripheral blood neutrophil concentrations in these mice are elevated as a result of accumulation of neutrophils in the circulation or altered hematopoiesis mediated by a disrupted regulatory feedback loop. Chimeric mice were generated by transplanting various ratios of CD18+/+ and CD18−/− unfractionated bone marrow cells into lethally irradiated wild-type mice, resulting in approximately 0%, 10%, 50%, 90%, or 100% CD18 null neutrophils in the blood. The presence of only 10% CD18+/+ neutrophils was sufficient to prevent the severe neutrophilia seen in mice reconstituted with CD18−/− bone marrow cells. These data show that the neutrophilia in CD18−/− mice is not caused by enhanced neutrophil survival or the inability of neutrophils to leave the vascular compartment. In CD18−/−, CD18−/−E−/−, CD18−/−P−/−, EP−/−, and EPI−/− mice, levels of granulocyte colony-stimulating factor (G-CSF) and interleukin-17 (IL-17) were elevated in proportion to the neutrophilia seen in these mice, regardless of the underlying mutation. Antibiotic treatment or the propensity to develop skin lesions did not correlate with neutrophil counts. Blocking IL-17 or G-CSF function in vivo significantly reduced neutrophil counts in severely neutrophilic mice by approximately 50% (P &lt; .05) or 70% (P &lt; .01), respectively. These data show that peripheral blood neutrophil numbers are regulated by a feedback loop involving G-CSF and IL-17 and that this feedback loop is disrupted when neutrophils cannot migrate into peripheral tissues.
Quantitative trait loci regulating relative lymphocyte proportions in mouse peripheral blood
Authors: Chen Jichun , Harrison David E. 
Published: July 2002

AbstractRelative proportions of peripheral blood (PB) B lymphocytes (B220%) as well as CD4 (CD4%) and CD8 (CD8%) T lymphocytes differ significantly among inbred mouse strains: B220% is high in C57BL/6J (B6) and C57BR/cdJ, intermediate in BALB/cByJ (BALB) and DBA/2J (D2), and low in NOD/LtJ (NOD) and SJL/J (SJL) mice, whereas CD4% and CD8% are high in NOD and SJL mice and low in the other 4 strains. By following segregating genetic markers linked to these traits in (B6 × D2) recombinant inbred (BXD RI) mice, the study defined 2 quantitative trait loci (QTLs) for the B220% phenotype:Pbbcp1 (peripheral blood B cell percentage 1, logarithm of odds [LOD] 4.1, P &lt; .000 01) and Pbbcp2(LOD 3.7, P &lt; .000 04) on chromosome 1 (Chr 1) at about 63 cM and 48 cM; one suggestive locus for the CD4% phenotype (LOD 2.6,P &lt; .000 57) on Chr 8 at about 73 cM; and one QTL for the CD8% phenotype: Pbctlp1 (peripheral blood cytotoxic T lymphocyte percentage 1, LOD 3.8, P &lt; .000 02) on Chr 19 at about 12 cM. The study further segregated PB lymphocyte proportions in B6SJLF2 mice by using DNA markers adjacent to these mapped QTLs and found that the Pbbcp1 locus (LOD 5.6,P &lt; .000 01) was also important in this mouse population. In both BXD RI and B6SJLF2 mice, QTLs regulating B-cell proportions showed no significant effect on T-cell proportions and vice versa. Thus, PB B- and T-lymphocyte proportions are regulated separately by different genetic elements.
PTEN controls immunoreceptor (immunoreceptor tyrosine-based activation motif) signaling and the activation of Rac
Authors: Kim Jong Suk , Peng Xiaodong , De Pradip K. , Geahlen Robert L. , Durden Donald L. 
Published: July 2002

AbstractFcγ receptor–mediated phagocytosis is a model for the study of immunoreceptor (immunoreceptor tyrosine-based activation motif [ITAM]) signaling and involves the activation of protein tyrosine kinases, protein tyrosine phosphatases, and downstream effectors including phosphatidylinositol-3 (PI-3) kinase. Relatively little is known of the role of lipid phosphatases in the control of ITAM signaling and inflammation. A heterologous COS7 cell system was used to examine the roles played by PI-3 kinase and the dual-specificity phosphatase, phosphatase and tensin homolog deleted on chromosome 10 (PTEN), in the signal transduction pathway leading to Fcγ receptor IIA–mediated phagocytosis and the activation of Rac. The expression of wildtype PTEN completely abrogated the phagocytosis of immunoglobulin-G–sensitized sheep red blood cells, as compared with the catalytically inactive mutant of PTEN, which had no effect. This is the first direct evidence that PTEN, an inositol 3′ phosphatase, regulates Fcγ receptor–mediated phagocytosis, an ITAM-based signaling event. The data suggest that PTEN exerts control over phagocytosis potentially by controlling the downstream conversion of guanosine diphosphate–Rac to guanosine triphosphate–Rac following ITAM stimulation.
Indirect Filtering Control for Interconnected Time‐Delay Systems With Deferred Constraints and Multiple Faults
Authors: You Guodong , Zhang Hailong , Li Xingyun , Wu Jinyuan , Liu Lu 
Published: September 2024

ABSTRACTIn this paper, an indirect filtering fault‐tolerant control method is proposed for interconnected time‐delay systems with multiple sensor and actuator faults. As is well known, directly utilizing the state information obtained from sensors with faults to constrain the actual state poses great challenges. This article utilizes conservative sensor fault loss information to indirectly apply time‐varying asymmetric constraints to the actual state while ensuring compliance with fault state constraints. In addition, the conservatism requirement of the initial state is reduced by utilizing carefully constructed delay constraint functions. To solve the problem of “explosion of complexity,” the derivative of the output signal of the filter is used to replace the derivative of the virtual control rate. The Nussbaum function can effectively alleviate the impact of multiple actuator failures and unknown control directions on the system. The effectiveness of the proposed control strategy is verified through simulation examples.
STAR Plus Scholarship Act announced
Authors: Knopf Alison 
Published: September 2024

On Sept. 12, U.S. Representatives Andrea Salinas (D‐Oregon) and Marc Molinaro (R‐New York) introduced the Substance Use Disorder Treatment and Recovery (STAR) Plus Scholarship Act. This bill aims to establish a new scholarship program for students studying in the fields of mental health, behavioral health, or substance use disorder treatment. The STAR Loan Repayment Program, administered by the Health Resources and Services Administration, has consistently advocated for the creation of a scholarship program to accompany the loan repayment program. “NAADAC is excited and proud to support the STAR Plus Scholarship Act, as creation of these scholarship opportunities is an essential step towards building a more robust and well‐trained addiction workforce,” said Terrence Walton, MSW, NAADAC's executive director. “NAADAC applauds Representatives Salinas and Molinaro for their leadership and recognition of the meaningful difference this policy solution will make in countless lives.”
Xinglou Chengqi Decoction Protects against Cerebral Ischemia/Reperfusion Injury by Inhibiting Ferroptosis via SLC7A11/GPX4 Signaling
Authors: Liu Hua , Yue Qiyu , Zhang Wenyue , Ding Qi , Yang Junjie , Lin Mu , Sun Jia 
Published: September 2024

AbstractXinglou Chengqi decoction (XLCQD) is a Chinese formula that offers benefits in ischemic stroke. However, the underlying mechanism of the effects of XLCQD‐mediated anti‐ischemic stroke effects remains obscure. This study investigates the ferroptosis mechanism of XLCQD against cerebral ischemia/reperfusion (I/R) injury using rat models of middle cerebral artery occlusion/reperfusion (MCAO/R). Ferroptosis differs from traditional cell death pathways and is linked to oxidative stress‐induced lipid peroxidation and glutathione (GSH) depletion, which is essential to the development of ischemic stroke. In this study, it is shown that XLCQD improves brain infarction, neurological dysfunction, and histopathological changes caused by MCAO/R exposure, and improving I/R‐induced oxidative damage through inhibition of ferroptosis via (Solute Carrier Family 7 Member 11) SLC7A11/ (glutathione peroxidase 4) GPX4 pathway. Interestingly, it is found that XLCQD‐mediated protection in I/R is reversed by the silence of SLC7A11. XLCQD intervention significantly promotes GSH content and suppresses Reactive Oxygen Species(ROS), iron accumulation, as well as Malondialdehyde (MDA) generation, are markedly abrogated when SLC7A11 is knockdown by SLC7A11‐shRNA transfection, indicating that SLC7A11 is the main target of XLCQD to further trigger intracellular events. In conclusion, XLCQD attenuates in vivo cerebral I/R injury by reducing ferroptosis via the SLC7A11/GPX4 pathway.
Guest‐Dependent Stimuli‐Responsive Photoluminescence in 0D Antimony Chlorides for Anticounterfeiting and Encryption Applications
Authors: Li Wenzhi , Mao Xin , Yin Hong , Wang Yuxi , Wang Yifan , Chen Junsheng , Han Keli , Zhang Ruiling 
Published: September 2024

Abstract0D lead‐free metal halides with stimuli‐responsive photoluminescence (PL) have attracted great attention as promising smart materials for advanced anticounterfeiting and information encryption. However, designing high‐performance stimuli‐responsive metal halides remains a challenge. Herein, a solvent guest tuning strategy to develop stimuli‐responsive 0D metal halides is proposed. Three novel highly yellow‐emissive host‐guest systems, TMA2SbCl5·X (TMA: (CH3)4N+, X = DMSO, DMF, and MeCN) are synthesized. Under heat stimuli, TMA2SbCl5·X (X = DMSO and DMF) exhibits PL on‐off switching, while TMA2SbCl5·MeCN shows PL color change from yellow to orange, due to structural phase transitions. Particularly, TMA2SbCl5·DMSO and TMA2SbCl5·MeCN demonstrate a fast response within 50 s at temperatures above 353 K. Detailed in situ XRD diffraction study unveils the role of guest solvent molecules in the regulation of structural phase transitions. For the first time, it is found that the host‐guest systems possess a guest‐dependent structural evolution that results in a different PL response to heat stimuli. In addition, moister can also trigger structural phase transition and induce PL on‐off switching. The remarkable stimuli response performance makes these hybrids promising for anticounterfeiting and encryption applications. These findings provide new insights for the development of stimuli‐responsive lead‐free metal halide materials.
Nucleotide‐Tackified Degradable and Closed‐Loop Recyclable Underwater Adhesive
Authors: Huang Mingsong , Liu Lingyu , Guo Wei , Cui Liying , Gao Guanghui , Zhang Qin , Liu Xin 
Published: September 2024

AbstractSustainable underwater adhesives have drawn much interest in promising applications and environmental engineering. However, developing a convenient strategy to combine recyclability and underwater adhesion into adhesive materials remains a formidable challenge. In this work, a degradable and closed‐loop recyclable underwater adhesive tackifier is designed by nucleotide, via the ring‐opening polymerization of thioctic acid in the presence of 5′‐adenosine monophosphate and methacrylatoethyl trimethyl ammonium chloride. The nucleotide‐tackified adhesives present an excellent underwater adhesion for various materials, tissues, and on‐demand detachment adhesion behavior. The underwater adhesives demonstrate reusability, degradability, and closed‐loop recyclability of dual monomers of thioctic acid and 5′‐adenosine monophosphate. Interestingly, the waste residues of the underwater adhesives demonstrate processability and can be employed again in the construction of tough adhesive interfaces in both air and underwater, with further expanding sustainability. It is envisioned that the strategy of nucleotide‐tackified sustainable underwater adhesives would offer promising insights for advancing sustainable and recyclable adhesive materials.
A Perspective on Electrochemical Point Source Utilization of CO2 and Other Flue Gas Components to Value Added Chemicals
Authors: Mondal Soumi , Peter Sebastian C. 
Published: September 2024

AbstractElectrochemical CO2 reduction reaction (eCO2RR) has been explored extensively for mitigation of noxious CO2 gas generating C1 and C2+ hydrocarbons and oxygenates as value‐added fuels and chemicals with remarkable selectivity. The source of CO2 being a pure CO2 feed, it does not fully satisfy the real‐time digestion of industrial exhausts. Besides the detrimental effect of noxious gas mixture leading to global warming, there is a huge capital investment in purifying the flue gas mixtures from industries. The presence of other impurity gases affects the eCO2RR mechanism and its activity and selectivity toward C2+ products dwindle drastically. Impurities like NOx, SOx, O2, N2, and halide ions present in flue gas mixture reduce the conversion and selectivity of eCO2RR significantly. Instead of wiping out these impurities via separation processes, new strategies from material chemistry and electrochemistry can open new avenues for turning foes to friends! In this perspective, the co‐electroreduction will vividly discussed and supporting role of different heteroatom‐containing impurity gases with CO2, generating highly stable C─N, C─S, C─X bonds, and highlight the existing limitations and providing probable solutions for attaining further success in this field and translating this to industrial exhaust streams.
Bioinspired Homonuclear Diatomic Iron Active Site Regulation for Efficient Antifouling Osmotic Energy Conversion
Authors: Li Zhe , Wu Donghai , Wang Qingchen , Zhang Qixiang , Xu Peng , Liu Fangning , Xi Shibo , Ma Dongwei , Lu Yizhong , Jiang Lei , Zhang Zhen 
Published: September 2024

AbstractMembrane‐based reverse electrodialysis is globally recognized as a promising technology for harnessing osmotic energy. However, its practical application is greatly restricted by the poor anti‐fouling ability of existing membrane materials. Inspired by the structural and functional models of natural cytochrome c oxidases (CcO), the first use of atomically precise homonuclear diatomic iron composites as high‐performance osmotic energy conversion membranes with excellent anti‐fouling ability is demonstrated. Through rational tuning of the atomic configuration of the diatomic iron sites, the oxidase‐like activity can be precisely tailored, leading to the augmentation of ion throughput and anti‐fouling capacity. Composite membranes featuring direct Fe‐Fe motif configurations embedded within cellulose nanofibers (CNF/Fe‐DACs‐P) surpass state‐of‐the‐art CNF‐based membranes with power densities of ca. 6.7 W m−2 and a 44.5‐fold enhancement in antimicrobial performance. Combined, experimental characterization and density functional theory simulations reveal that homonuclear diatomic iron sites with metal‐metal interactions can achieve ideally balanced adsorption and desorption of intermediates, thus realizing superior oxidase‐like activity, enhanced ionic flux, and excellent antibacterial activity.
Emerging Opportunities of Colloidal Quantum Dots for Photocatalytic Organic Transformations
Authors: Cao Qinxuan , Feng Jianning , Chang Kin Ting , Liang Wenfei , Lu Haipeng 
Published: September 2024

AbstractColloidal quantum dots (QDs) have emerged as a versatile photocatalyst for a wide range of photocatalytic transformations owing to its high absorption coefficient, large surface‐to‐volume ratio, high stability, and efficient charge and energy transfer dynamics. The past decades have witnessed a rapid development of QDs for artificial photocatalysis. In this review, the unique characteristics of QDs are focused on, including quantum size effect, compositional and structural diversity, tunable surface chemistry, and photophysics, that can be utilized for photocatalytic transformations. The recent advancements in photocatalytic organic transformations enabled by QDs photocatalysts are summarized. The unique opportunities of QDs are highlighted to tackle organic reactions that are previously unattainable with small molecule photocatalysts. Lastly, an outlook is provided for future directions in this field.
Photochemical Control of Network Topology in PEG Hydrogels
Authors: Kirkpatrick Bruce E. , Hach Grace K. , Nelson Benjamin R. , Skillin Nathaniel P. , Lee Joshua S. , Hibbard Lea Pearl , Dhand Abhishek P. , Grotheer Henry S. , Miksch Connor E. , Salazar Violeta , Hebner Tayler S. , Keyser Sean P. , Kamps Joshua T. , Sinha Jasmine , Macdougall Laura J. , Fairbanks Benjamin D. , Burdick Jason A. , White Timothy J. , Bowman Christopher N. , Anseth Kristi S. 
Published: September 2024

AbstractHydrogels are often synthesized through photoinitiated step‐, chain‐, and mixed‐mode polymerizations, generating diverse network topologies and resultant material properties that depend on the underlying network connectivity. While many photocrosslinking reactions are available, few afford controllable connectivity of the hydrogel network. Herein, a versatile photochemical strategy is introduced for tuning the structure of poly(ethylene glycol) (PEG) hydrogels using macromolecular monomers functionalized with maleimide and styrene moieties. Hydrogels are prepared along a gradient of topologies by varying the ratio of step‐growth (maleimide dimerization) to chain‐growth (maleimide‐styrene alternating copolymerization) network‐forming reactions. The initial PEG content and final network physical properties (e.g., modulus, swelling, diffusivity) are tailored in an independent manner, highlighting configurable gel mechanics and reactivity. These photochemical reactions allow high‐fidelity photopatterning and 3D printing and are compatible with 2D and 3D cell culture. Ultimately, this photopolymer chemistry allows facile control over network connectivity to achieve adjustable material properties for broad applications.
Sustainable Nanofibril Interfaces for Strain‐Resilient and Multimodal Porous Bioelectronics
Authors: Zhao Ganggang , Chen Zehua , Wang Shaoyun , Chen Sicheng , Zhang Feng , Andrabi Syed Muntazir , Xu Yadong , Ouyang Qunle , Rosas Milton Eric Busquets , Qian Xiaoyan , Xie Jingwei , Yan Zheng 
Published: September 2024

AbstractPorous soft bioelectronics have attracted significant attention due to their high breathability, long‐term biocompatibility, and other unique features inaccessible in nonporous counterparts. However, fabricating high‐quality multimodal bioelectronic components that operate stably under strain on porous substrates, along with integrating microfluidics for sweat management, remains challenging. In this study, cellulose nanofibrils (CNF) are explored, biomass‐derived sustainable biomaterials, as nanofibril interfaces with unprecedented interfacial robustness to enable high‐quality printing of strain‐resilient bioelectronics on porous substrates by reducing surface roughness and creating mechanical heterogeneity. Also, CNF‐based microfluidics can provide continuous sweat collection and refreshment, crucial for accurate biochemical sensing. Building upon these advancements, a multimodal porous wearable bioelectronic system is further developed capable of simultaneously detecting electrocardiograms and glucose and beta‐hydroxybutyrate in sweat for monitoring energy metabolism and consumption. This work introduces novel strategies for fabricating high‐quality, strain‐resilient porous bioelectronics with customizable multimodalities to meet arising personalized healthcare needs.