bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical accuracy of abnormal cell‐free fetal DNA results for the sex chromosomes
Authors: Scibetta Emily W. , Gaw Stephanie L. , Rao Rashmi R. , Silverman Neil S. , Han Christina S. , Platt Lawrence D. 
Published: August 2017

AbstractObjectiveTo investigate factors associated with abnormal cell‐free DNA (cfDNA) results for sex chromosomes (SCs).Study designThis is a retrospective cohort study of abnormal cfDNA results for SC at a referral practice from March 2013 to July 2015. Cell‐free DNA results were abnormal if they were positive for SC aneuploidy (SCA), inconclusive, or discordant with ultrasound (US) findings. Primary outcome was concordance with karyotype or postnatal evaluation.ResultsOf 50 abnormal cfDNA results for SC, 31 patients (62%) were positive for SCA, 13 (26%) were inconclusive, and 6 (12%) were sex discordant on US. Of SCA results, 19 (61%) were reported as 45,X and 12 (39%) were SC trisomy. Abnormal karyotypes were confirmed in 8/23 (35%) of SC aneuploidy and 1/5 (20%) of inconclusive results. Abnormal SC cfDNA results were associated with in vitro fertilization (P = .001) and twins (P < .001). Sex discordance between cfDNA and US was associated with twin gestation (P < .001).ConclusionsIn our cohort, abnormal SC cfDNA results were associated with in vitro fertilization and twins. Our results indicate cfDNA for sex prediction in twins of limited utility. Positive predictive value and sensitivity for SC determination were lower than previously reported.
Stillbirth and neonatal mortality in pregnancies complicated by major congenital anomalies: Findings from a large European cohort
Authors: Groen Henk , Bouman Katelijne , Pierini Anna , Rankin Judith , Rissmann Anke , Haeusler Martin , Yevtushok Lyubov , Loane Maria , Erwich Jan Jaap H.M. , de Walle Hermien E.K. 
Published: August 2017

AbstractObjectiveTo provide prognostic information to help parents to reach an informed decision about termination or continuation of the pregnancy and to shape peripartum policy based on a large European cohort.MethodThirteen registries from the European Surveillance of Congenital Anomalies (EUROCAT) network contributed data from January 1, 1998, to December 31, 2011. Terminations for fetal anomalies were excluded. Chromosomal anomalies, syndromes and isolated anomaly groups were distinguished according to EUROCAT guidelines. Perinatal mortality, stillbirths, and early and late neonatal mortality rates (NMRs) were analyzed by anomaly group and gestational age.ResultsAmong 73 337 cases, perinatal mortality associated with congenital anomaly was 1.27 per 1000 births (95% confidence interval, 1.23‐1.31). Average stillbirth rate was 2.68% (range 0%‐51.2%). Early and late NMR were 2.75% (range 0%‐46.7%) and 0.97% (range 0%‐17.9%), respectively. Chromosomal anomalies and syndromes, and most isolated anomalies, had significant differences regarding timing of fetal demise compared to the general population. Chromosomal and central nervous system anomalies had higher term stillbirth rates.ConclusionsWe found relevant differences between anomalies regarding rates of stillbirth, NMR, and timing by gestational age. Our data can help parents to decide about their unborn child with a congenital anomaly and help inform maternal‐fetal medicine specialists regarding peripartum management.
Exposure to BMAA mirrors molecular processes linked to neurodegenerative disease
Authors: Beri Joshua , Nash Tara , Martin Rubia M. , Bereman Michael S. 
Published: August 2017

AbstractThe goal of this study is to investigate the molecular pathways perturbed by in vitro exposure of beta‐methylamino‐L‐alanine (BMAA) to NSC‐34 cells via contemporary proteomics. Our analysis of differentially regulated proteins reveals significant enrichment (p < 0.01) of pathways related to ER stress, protein ubiquitination, the unfolded protein response, and mitochondrial dysfunction. Upstream regulator analysis indicates that exposure to BMAA induces activation of transcription factors (X‐box binding protein 1; nuclear factor 2 erythroid like 2; promyelocytic leukemia) involved in regulation of the UPR, oxidative stress, and cellular senescence. Furthermore, the authors examine the hypothesis that BMAA causes protein damage via misincorporation in place of L‐Serine. The authors are unable to detect misincorporation of BMAA into protein via analysis of cellular protein, secreted protein, targeted detection of BMAA after protein hydrolysis, or through the use of in vitro protein translation kits.
CCBuilder 2.0: Powerful and accessible coiled‐coil modeling
Authors: Wood Christopher W. , Woolfson Derek N. 
Published: August 2017

AbstractThe increased availability of user‐friendly and accessible computational tools for biomolecular modeling would expand the reach and application of biomolecular engineering and design. For protein modeling, one key challenge is to reduce the complexities of 3D protein folds to sets of parametric equations that nonetheless capture the salient features of these structures accurately. At present, this is possible for a subset of proteins, namely, repeat proteins. The α‐helical coiled coil provides one such example, which represents ≈ 3–5% of all known protein‐encoding regions of DNA. Coiled coils are bundles of α helices that can be described by a small set of structural parameters. Here we describe how this parametric description can be implemented in an easy‐to‐use web application, called CCBuilder 2.0, for modeling and optimizing both α‐helical coiled coils and polyproline‐based collagen triple helices. This has many applications from providing models to aid molecular replacement for X‐ray crystallography, in silico model building and engineering of natural and designed protein assemblies, and through to the creation of completely de novo “dark matter” protein structures. CCBuilder 2.0 is available as a web‐based application, the code for which is open‐source and can be downloaded freely. http://coiledcoils.chm.bris.ac.uk/ccbuilder2.Lay SummaryWe have created CCBuilder 2.0, an easy to use web‐based application that can model structures for a whole class of proteins, the α‐helical coiled coil, which is estimated to account for 3–5% of all proteins in nature. CCBuilder 2.0 will be of use to a large number of protein scientists engaged in fundamental studies, such as protein structure determination, through to more‐applied research including designing and engineering novel proteins that have potential applications in biotechnology.
Parameterized LMIs for robust and state feedback control of continuous‐time polytopic systems
Authors: Rodrigues L.A. , Oliveira R.C.L.F. , Camino J.F. 
Published: August 2017

SummaryThis paper presents new extended linear matrix inequality (LMI) characterizations for the synthesis of robust
and
state feedback controllers for continuous‐time linear time‐invariant systems with polytopic uncertainty. Based on a suitable change of variables and the Elimination Lemma, the proposed robust control design techniques are stated as extended LMI conditions parameterized in terms of 2 scalar parameters. One parameter is shown to belong to a bounded domain, thus limiting the scalar search domain. For the other parameter, a bounded subset is provided from numerical experiments. The benefits of the methodology are illustrated through numerical simulations performed on an uncertain model borrowed from the literature. It is shown that the proposed LMI relaxations can provide less conservative results with fewer scalar searches than some existing methods in the literature.
Concise Review: Fetal Membranes in Regenerative Medicine: New Tricks from an Old Dog?
Authors: Lim Rebecca 
Published: August 2017

AbstractThe clinical application of the fetal membranes dates back to nearly a century. Their use has ranged from superficial skin dressings to surgical wound closure. The applications of the fetal membranes are constantly evolving, and key to this is the uncovering of multiple populations of stem and stem-like cells, each with unique properties that can be exploited for regenerative medicine. In addition to pro-angiogenic and immunomodulatory properties of the stem and stem-like cells arising from the fetal membranes, the dehydrated and/or decellularized forms of the fetal membranes have been used to support the growth and function of other cells and tissues, including adipose-derived mesenchymal stem cells. This concise review explores the biological origin of the fetal membranes, a history of their use in medicine, and recent developments in the use of fetal membranes and their derived stem and stem-like cells in regenerative medicine.
Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication
Authors: Leberfinger Ashley N. , Ravnic Dino J. , Dhawan Aman , Ozbolat Ibrahim T. 
Published: August 2017

Abstract
Bioprinting is a quickly progressing technology, which holds the potential to generate replacement tissues and organs. Stem cells offer several advantages over differentiated cells for use as starting materials, including the potential for autologous tissue and differentiation into multiple cell lines. The three most commonly used stem cells are embryonic, induced pluripotent, and adult stem cells. Cells are combined with various natural and synthetic materials to form bioinks, which are used to fabricate scaffold-based or scaffold-free constructs. Computer aided design technology is combined with various bioprinting modalities including droplet-, extrusion-, or laser-based bioprinting to create tissue constructs. Each bioink and modality has its own advantages and disadvantages. Various materials and techniques are combined to maximize the benefits. Researchers have been successful in bioprinting cartilage, bone, cardiac, nervous, liver, and vascular tissues. However, a major limitation to clinical translation is building large-scale vascularized constructs. Many challenges must be overcome before this technology is used routinely in a clinical setting.
A Label‐Free Fluorescent Sensor Based on Structure‐Switching Oligonucleotides for the Detection of Ag+, Biothiols and Acetylcholinesterase Activity
Authors: Zhang Baozhu , Wei Chunying 
Published: August 2017

AbstractA label‐free, highly sensitive and specific fluorescent sensor was developed for the detection of Ag+, biothiols and acetylcholinesterase (AChE) activity and for the screening of AChE inhibitors. The sensing approach was based on the conformational switch of human telomeric G‐rich sequence. The present assay was capable of detecting Ag+ in the range of 0 to 6.0 μM, with a detection limit of 34 nM. In addition, the proposed method was developed as a turn‐on cysteine‐sensing system, which presented a good response to cysteine in the range of 0 to 4.5 μM, and the detection limit was 25 nM. The cysteine‐sensing system could be used to detect the activity of AChE as low as 0.05 U/L in the range of 0.05 to 1.3 U/L. The proposed sensor was also promising in screening of AChE inhibitors. Furthermore, this method has been applied successfully to the sensing of Ag+ in tap water and lake water with satisfactory recovery.
Effect of the Electrolyte Alkaline Ions on the Electrochemical Performance of α‐Ni(OH)2/Activated Carbon Composites in the Hybrid Supercapacitor Cell
Authors: Soserov L. , Boyadzhieva T. , Koleva V. , Girginov Ch. , Stoyanova A. , Stoyanova R. 
Published: August 2017

AbstractHybrid electrochemical supercapacitors supply a high energy density and reduced cycle stability. Herein, we provide new data on the effect of the Li+ and K+ ion from the aqueous alkaline electrolyte on the hybrid supercapacitor performance. The electrochemical cell is assembled by two dissimilar electrodes: AC versus α‐Ni(OH)2 /AC composite. Two types of alkaline electrolytes are used: individual KOH and mixed LiOH‐KOH solutions. The performance of the composite electrodes is tested by galvanostatic and cyclic voltammetry experiments. The Li+ ions from the electrolyte display a positive effect on the discharge capacitance and the cycling stability of α‐Ni(OH)2/AC. The improved performance is discussed on the basis of structure and morphology changes of the composite electrode after electrochemical testing that are probed by ex‐situ X‐ray Diffraction, Scanning Electron Microscopy and Atomic‐force Microscopy. The results show that Li+ ions and H2O from the LiOH‐KOH electrolyte are intercalated into α‐Ni(OH)2. The surface of the composite electrode becomes smoother in the mixed electrolyte. As a result, the α‐Ni(OH)2/AC displays an improved performance in the mixed LiOH‐KOH electrolyte than that in the individual KOH electrolyte.
Reduced Nrf2 activation in PI3K phosphorylation‐impaired vitiliginous keratinocytes increases susceptibility to ROS‐generating chemical‐induced apoptosis
Authors: Kim Hyangmi , Park Chang Seo , Lee Ai‐Young 
Published: August 2017

AbstractKeratinocytes in affected epidermis of vitiligo patients are known to have impaired activation of the PI3K/AKT pathway. Based on critical roles of keratinocytes and oxidative stress in vitiligo development, this study examined whether keratinocytes with impaired PI3K activation were more vulnerable to apoptosis caused by oxidative stress from phenolic compounds, p‐tert‐butylphenol (4‐TBP) and hydroquinone (HQ). Cell viability assay, FACS analysis, ELISA for TNF‐α or IL‐1a, ROS assay, Western blot analysis for Nrf2 expression, and confocal microscopy with anti‐Nrf2 and phospho‐PI3K antibodies were done on primary cultured normal human keratinocytes with or without PI3K knockdown in the presence or absence of chemical treatment or antioxidant. Immunofluorescence staining using anti‐Nrf2, phospho‐PI3K, TNF‐ɑ, and IL‐1ɑ antibodies, ROS assay using dihydroethidium, and TUNEL assay were performed on sets of depigmented and normally pigmented skin from vitiligo patients. Results showed that 4‐TBP or HQ treatment increased apoptosis and the expression levels of TNF‐ɑ, IL‐1ɑ, and ROS in PI3K‐knockdown keratinocytes which reduced Nrf2 nuclear translocation compared to control keratinocytes. These changes were significantly recovered by an antioxidant treatment. Depigmented epidermis of vitiligo patients also showed lower levels of Nrf2 and phospho‐PI3K but higher levels of ROS, TNF‐ɑ, IL‐1ɑ, and ROS with more TUNEL‐positive cells. Therefore, impaired PI3K activation in keratinocytes in depigmented epidermis of vitiligo patients are vulnerable to apoptosis caused by ROS‐generating chemicals due to reduced Nrf2 activation.
Advances in metabolome information retrieval: turning chemistry into biology. Part I: analytical chemistry of the metabolome
Authors: Tebani Abdellah , Afonso Carlos , Bekri Soumeya 
Published: August 2017

AbstractMetabolites are small molecules produced by enzymatic reactions in a given organism. Metabolomics or metabolic phenotyping is a well‐established omics aimed at comprehensively assessing metabolites in biological systems. These comprehensive analyses use analytical platforms, mainly nuclear magnetic resonance spectroscopy and mass spectrometry, along with associated separation methods to gather qualitative and quantitative data. Metabolomics holistically evaluates biological systems in an unbiased, data‐driven approach that may ultimately support generation of hypotheses. The approach inherently allows the molecular characterization of a biological sample with regard to both internal (genetics) and environmental (exosome, microbiome) influences. Metabolomics workflows are based on whether the investigator knows a priori what kind of metabolites to assess. Thus, a targeted metabolomics approach is defined as a quantitative analysis (absolute concentrations are determined) or a semiquantitative analysis (relative intensities are determined) of a set of metabolites that are possibly linked to common chemical classes or a selected metabolic pathway. An untargeted metabolomics approach is a semiquantitative analysis of the largest possible number of metabolites contained in a biological sample. This is part I of a review intending to give an overview of the state of the art of major metabolic phenotyping technologies. Furthermore, their inherent analytical advantages and limits regarding experimental design, sample handling, standardization and workflow challenges are discussed.
Physician Experiences and Understanding of Genomic Sequencing in Oncology
Authors: Weipert Caroline M. , Ryan Kerry A. , Everett Jessica N. , Yashar Beverly M. , Chinnaiyan Arul M. , Scott Roberts J. , De Vries Raymond , Zikmund‐Fisher Brian J. , Raymond Victoria M. 
Published: August 2017

AbstractThe amount of information produced by genomic sequencing is vast, technically complicated, and can be difficult to interpret. Appropriately tailoring genomic information for non‐geneticists is an essential next step in the clinical use of genomic sequencing. To initiate development of a framework for genomic results communication, we conducted eighteen qualitative interviews with oncologists who had referred adult cancer patients to a matched tumor‐normal tissue genomic sequencing study. In our qualitative analysis, we found varied levels of clinician knowledge relating to sequencing technology, the scope of the tumor genomic sequencing study, and incidental germline findings. Clinicians expressed a perceived need for more genetics education. Additionally, they had a variety of suggestions for improving results reports and possible resources to aid in results interpretation. Most clinicians felt genetic counselors were needed when incidental germline findings were identified. Our research suggests that more consistent genetics education is imperative in ensuring the proper utilization of genomic sequencing in cancer care. Clinician suggestions for results interpretation resources and results report modifications could be used to improve communication. Clinicians’ perceived need to involve genetic counselors when incidental germline findings were found suggests genetic specialists could play a critical role in ensuring patients receive appropriate follow‐up.
Universidades influyentes en investigación sobre orientación al mercado. Una visión general entre 1990 y 2014
Authors: Valenzuela Fernández Leslier , Merigó José M. , Nicolas Carolina 
Published: August 2017

El objetivo de este estudio es identificar las universidades más productivas e influyentes para la comunidad científica sobre el tópico de orientación al mercado. Lo anterior se realiza principalmente a través de indicadores bibliométricos —como el índice h— y la relación total citas/total artículos para el periodo 1990-2014, a partir de la información encontrada en Web of Science. Dentro de los hallazgos se destaca el interés de la comunidad científica en esta temática, lo que se ve reflejado en el aumento considerado en la contribución que se ha generado durante los últimos 25 años. Además, se determina un ranking de las 30 universidades más influyentes, junto con un ranking que relaciona universidades y revistas con mayor influencia en temas de orientación al mercado.
Conservatism as a situated identity: Implications for consumer behavior
Authors: Oyserman Daphna , Schwarz Norbert 
Published: August 2017

AbstractInsufficient attention to political ideology as an organizing axis reduces predictive power. Jost (2017 – this issue) makes a significant contribution by outlining and documenting a set of relationships among personality factors, attitudes, values, and conservatism. The value of this approach is highlighting the possibility that ideology sticks when it fits features of the individual and hence has an enduring quality. This approach needs to be balanced by consideration of the power of the immediate situation to define what an identity means and the potential universality of many features associated with conservatism. We discuss both issues using identity‐based motivation theory as our organizing framework.
Ethical Climates in Organizations: A Review and Research Agenda
Authors: Newman Alexander , Round Heather , Bhattacharya Sukanto , Roy Achinto 
Published: August 2017

ABSTRACT:Since seminal meta-analytical work in 2006 we have witnessed burgeoning research on ethical climates. This article offers a comprehensive review of literature examining the antecedents and outcomes of ethical climates over the last decade, as well as moderators of the relationship between ethical climates and other variables. Based on the review, an agenda for future research is also presented. In addition to highlighting the potential for incorporating alternative theoretical perspectives such as situational strength theory, trait-activation theory, social information processing theory, and institutional theory to better our understanding of ethical climates, this article highlights the need for future research to incorporate a dynamic perspective to study ethical climates, examine the curvilinear effects of ethical climates on work outcomes, extend the study of ethical climates to different levels of the organization, and examine the effects of culture on ethical climates.
Generalized Two Temperatures Thermoelasticity of Micropolar Porous Circular Plate with Three Phase Lag Model
Authors: Kumar R. , Miglani A. , Rani R. 
Published: August 2017

AbstractThe present study is to focus on the two dimensional problem of micropolar porous circular plate with three phase lag model within the context of two temperatures generalized thermoelasticity theory. The problem is solved by applying Laplace and Hankel transforms after using potential functions. The expressions of displacements, microrotation, volume fraction field, temperature distribution and stresses are obtained in the transformed domain. To show the utility of the approach, normal force and thermal source are taken. The numerical inversion techniques of transforms have been carried out in order to evaluate the resulting quantities in the physical domain. Finally, the resulting quantities are depicted graphically to show the effect of porosity, two temperatures and phase lags.
Derivation of Non-Newtonian Magnetic Fluid Lubricated Rough Centrosymmetric Squeeze Film Reynolds Equation and its Application
Authors: Lin J. R. , Chu L. M. , Chiang H. L. , Chiu Y. K. 
Published: August 2017

AbstractBased upon the Shliomis ferromagnetic fluid model and the Stokes microcontinuum theory incorporating with the Christensen stochastic model, a modified Reynolds equation of centrosymmetric squeeze films has been derived in this paper. The Reynolds equation includes the combined effects of non-Newtonian rheology, magnetic fluids with applied magnetic fields, rotational inertia forces, and surface roughness. To guide the use of the derived equation, the squeeze film of rotational rough-surface circular disks lubricated with non-Newtonian magnetic fluids is illustrated. According to the results obtained, the effects of rotation inertia decrease the load capacity and the squeeze film time of smooth circular disks. By the use of non-Newtonian magnetic fluids with applied magnetic fields, the rotational circular disks predict better squeeze film performances. When the influences of circumferential roughness patterns are considered, the non-Newtonian magnetic-fluid lubricated rotational rough disks with applied magnetic fields provide further higher values of the load capacity and the squeeze film time as compared to those of the smooth case.