bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development
Authors: de la Porte Alexandra , Schröder Julia , Thomas Moritz , Geuder Johanna , Sterr Michael , Pastor Xavier , Sanderson Leslie E. , Barakat Tahsin Stefan , Enard Wolfgang , Marr Carsten , Schröter Christian , Drukker Micha 
Published: September 2024

AbstractEmbryos from different mammalian species develop at characteristic timescales. These timescales are recapitulated during the differentiation of pluripotent stem cellsin vitro. Specific genes and molecular pathways that modulate cell differentiation speed between mammalian species remain to be determined. Here we use single-cell multi-omic analysis of neural differentiation of mouse, cynomolgus and human pluripotent cells to identify regulators for differentiation speed. We demonstrate that species-specific transcriptome dynamics are mirrored at the chromatin level, but that the speed of neural differentiation is insensitive to manipulations of cell growth and cycling. Exploiting the single-cell resolution of our data, we identify glycogen storage levels regulated by UDP-glucose pyrophosphorylase 2 (UGP2) as a species-dependent trait of pluripotent cells, and show that lowered glycogen storage in UGP2 mutant cells is associated with accelerated neural differentiation. The control of energy storage could be a general strategy for the regulation of cell differentiation speed.
Phonetic underpinnings of sound symbolism across multiple domains of meaning
Authors: Lacey Simon , Matthews Kaitlyn L. , Sathian K. , Nygaard Lynne C. 
Published: September 2024

ABSTRACTSound symbolism occurs when the sound of a word alone can convey its meaning, e.g. ‘balloon’ and ‘spike’ sound rounded and pointed, respectively. Sound-symbolic correspondences are widespread in natural languages, but it is unclear how they are instantiated across different domains of meaning. Here, participants rated auditory pseudowords on opposing scales of seven different sound-symbolic domains: shape (rounded-pointed), texture (hard-soft), weight (light-heavy), size (small-big), brightness (bright-dark), arousal (calming-exciting), and valence (good-bad). Ratings showed cross-domain relationships, some mirroring those between corresponding physical domains, e.g. size and weight ratings were associated, reflecting a physical size-weight relationship, while others involved metaphorical mappings, e.g., bright/dark mapped onto good/bad, respectively. The phonetic features of the pseudowords formed unique sets with characteristic feature weightings for each domain and tended to follow the cross-domain ratings relationships. These results suggest that sound-symbolic correspondences rely on domain-specific patterns of phonetic features, with cross-domain correspondences reflecting physical or metaphorical relationships.
USP37 protects mammalian cells during DNA replication stress by counteracting CUL2LRR1and TRAIP
Authors: Villa Fabrizio , Ainsworth Johanna , Labib Karim P.M. 
Published: September 2024

SummaryThe USP37 deubiquitylase is important for mammalian cells to survive DNA replication stress but the underlying mechanisms are unknown. Here we demonstrate that USP37 binds the CDC45-MCM-GINS (CMG) helicase, which forms the stable core of the replisome and is regulated by ubiquitylation. The Pleckstrin-Homology Domain of USP37 binds CDC45 and structure-guided mutations that displace USP37 from CMG are sufficient to phenocopy loss of USP37 catalytic activity. Importantly, USP37 counteracts CMG helicase ubiquitylation by the CUL2LRR1ligase, which induces helicase disassembly during termination. We show that depletion of CUL2LRR1suppresses the sensitivity of Usp37 mutants to DNA synthesis defects and to ATR checkpoint kinase inhibitors. In contrast, mutation of the TRAIP ubiquitin ligase specifically suppresses the sensitivity of Usp37 mutants to topological stress during chromosome replication. We propose that USP37 evolved to reverse the untimely action of the two ubiquitin ligases that regulate the CMG helicase during chromosome replication in metazoa.
Oxygen-induced stress reveals context-specific gene regulatory effects in human brain organoids
Authors: Umans Benjamin D. , Gilad Yoav 
Published: September 2024

AbstractThe interaction between genetic variants and environmental stressors is key to understanding the mechanisms underlying neurological diseases. In this study, we used human brain organoids to explore how varying oxygen levels expose context-dependent gene regulatory effects. By subjecting a genetically diverse panel of 21 brain organoids to hypoxic and hyperoxic conditions, we identified thousands of gene regulatory changes that are undetectable under baseline conditions, with 1,745 trait-associated genes showing regulatory effects only in response to oxygen stress. To capture more nuanced transcriptional patterns, we employed topic modeling, which revealed context-specific gene regulation linked to dynamic cellular processes and environmental responses, offering a deeper understanding of how gene regulation is modulated in the brain. These findings underscore the importance of genotype-environment interactions in genetic studies of neurological disorders and provide new insights into the hidden regulatory mechanisms influenced by environmental factors in the brain.
BRD4 phosphorylation regulates the structure of chromatin nanodomains
Authors: Seitz Clayton , Fu Donghong , Liu Mengyuan , Ma Hailan , Liu Jing 
Published: September 2024

ABSTRACTThe interplay between chromatin structure and phase-separating proteins is an emerging topic in cell biology with implications for understanding disease states. Here, we investigate the functional relationship between bromodomain protein 4 (BRD4) and chromatin architecture. By combining molecular dynamics simulations with live-cell imaging, we demonstrate that BRD4, when mutated at specific N-terminus sites, significantly impacts nucleosome nanodomain (NN) organization and dynamics. Our findings reveal that enhanced chromatin binding activity of BRD4 condenses NNs, while both loss or gain of BRD4 chromatin binding reduced diffusion of single nucleosomes, suggesting a role for BRD4 in the regulation of nanoscale chromatin architecture and the chromatin microenvironment. These observations shed light on the nuanced regulation of chromatin structure by BRD4, offering insights into its role in maintaining the nuclear architecture and transcriptional activity.
Latent infection of an active giant endogenous virus in a unicellular green alga
Authors: Erazo-Garcia Maria P. , Sheyn Uri , Barth Zachary K. , Craig Rory J. , Wessman Petronella , Jivaji Abdeali M. , Ray W. Keith , Svensson-Coelho Maria , Cornwallis Charlie K. , Rengefors Karin , Brussaard Corina P. D. , Moniruzzaman Mohammad , Aylward Frank O. 
Published: September 2024

AbstractLatency is a common strategy in a wide range of viral lineages, but its prevalence in giant viruses remains unknown. Here we describe the activity and viral production from a 617 kbp integrated giant viral element in the model green algaChlamydomonas reinhardtii. We resolve the integrated viral region using long-read sequencing and show that viral particles are produced and released in otherwise healthy cultures. A diverse array of viral-encoded selfish genetic elements are expressed during GEVE reactivation and produce proteins that are packaged in virions. In addition, we show that field isolates ofChlamydomonassp. harbor latent giant viruses related to theC. reinhardtiiGEVE that exhibit similar infection dynamics, demonstrating that giant virus latency is prevalent in natural host communities. Our work reports the largest temperate virus documented to date and the first active GEVE identified in a unicellular eukaryote, substantially expanding the known limits of viral latency.
Promoting counter-hedonic entertainment in China: assessing the effectiveness of the free trial market strategy
Authors: Zhang Ziqiong , Sun Peiliang , Wang Le , Zhang Zili 
Published: September 2024

PurposeCounter-hedonic entertainment, characterized by evoking negative emotions in consumers, has emerged as one of the most popular and profitable forms of entertainment in Western countries. However, this entertainment type is not as popular in China as in Western countries. This study examines whether the free trial of counter-hedonic entertainment can promote the consumption of such entertainment and explores the moderating effect of contextual factors on this relationship.Design/methodology/approachThis study investigates the promotional effectiveness of the free trial strategy on counter-hedonic entertainment through a quasi-experiment involving the broadcasting of a large-scale free counter-hedonic program. This study has analyzed the counter-hedonic consumption data from 31 major cities in China and employed econometric modeling to test the hypotheses.FindingsResults show that the free trial of counter-hedonic entertainment can promote the sales of entertainment with similar categories. The follow-up moderation analyses show that the positive effect is amplified on days with less sunlight and for consumers with abundant (vs scarce) financial resources, thereby providing evidence to support the underlying drivers of the positive effect – the need for arousal and confidence frame. Meanwhile, the free trial of counter-hedonic entertainment also has a long-term effect after airing.Originality/valueBased on existing research on consumers’ counter-hedonic consumption motivation, this study confirms the effectiveness and boundary conditions of the free trial strategy in promoting counter-hedonic entertainment consumption for the first time. The results also offer actionable insights for counter-hedonic entertainment marketers and practitioners.
Third actor introductions to interaction episodes aiming at fast-forwarding new firm relationship development
Authors: Hassel John-Erik 
Published: September 2024

Purpose
This paper aims to address third actor introductions to interaction episodes aiming at fast-forwarding the continuous development of business relationships of new firms.

Design/methodology/approach
The study is qualitative, collecting data from 30 interviews from 28 informants associated with creation of new ventures and business network development in the context of a novel type of third actor called venture builder. Venture builders are privately owned organizations devoted to new firm creation in a factory-like mode, collaborating with individual entrepreneurs.

Findings
The findings suggest that interaction episodes, central to the development of new relationships, may be triggered by introductions managed by third actors using different types of involvement depending on the location and focus of the potential relationship. A framework is presented including four types of introductions to interaction episodes, aiming at saving time by removing the perceived distance between new firms and their counterparts in the initiation of business relationships. The framework describes four types of introductions of interaction episodes: Managed, Advised, Facilitated and Monitored.

Originality/value
Triggers and introductions of interaction episodes for new firms has previously been sparsely addressed. This paper presents how third actor involvement, by the introductions of interaction episodes with internal and external counterparts is managed with an aim of fast-forwarding relationship development.
Stablecoins and inflation in Latin America: the case of Argentina
Authors: Garita Mauricio , Cerezo Bregni Celso Fernando , Asturias Rodrigo 
Published: September 2024

PurposeThe purpose of this academic paper is to analyze Argentina’s inflationary situation through an understanding of its monetary policy over the years, and to identify its effect on the country’s poverty, explaining the relationship between fiat currencies and stable currencies.Design/methodology/approachBy analyzing the case of Argentina through descriptive methodology, the authors provide information on the use of stable currencies in Argentina and the reasons behind their use.FindingsThrough descriptive research, the authors were able to find out the situation regarding the use of stable currencies in Argentina. We identified how the country’s monetary policy has affected inflation and thus purchasing power parity.Research limitations/implicationsGiven that cryptocurrency information is based on privacy, there are certain arguments that must be referred through qualitative aspects.Practical implicationsThe importance of stablecoins in high inflation countries.Social implicationsThe understanding on how cryptocurrencies are able to maintain purchasing power and help avoid inflation related poverty.Originality/valueDiscussion of cryptocurrency items, specifically stablecoins, has been limited due to their recent emergence and the existing discussion about their legality. The study presents an argument on the use of stablecoins by presenting a case that has not yet been studied.
The role of endospore appendages in spore–spore interactions in the pathogenic Bacillus cereus group
Authors: Jonsmoen Unni Lise , Malyshev Dmitry , Sleutel Mike , Kristensen Elise Egeli , Zegeye Ephrem Debebe , Remaut Han , Andersson Magnus , Aspholm Marina Elisabeth 
Published: September 2024

AbstractSpecies within the Bacillus cereus sensu lato group, known for their spore‐forming ability, are recognized for their significant role in food spoilage and food poisoning. The spores of B. cereus are adorned with numerous pilus‐like appendages, referred to as S‐ENAs and L‐ENAs. These appendages are thought to play vital roles in self‐aggregation, adhesion, and biofilm formation. Our study investigates the role of S‐ENAs and L‐ENAs, as well as the impact of various environmental factors on spore‐to‐spore contacts and the interaction between spores and vegetative cells, using both bulk and single‐cell approaches. Our findings indicate that ENAs, especially their tip fibrillae, play a crucial role in spore self‐aggregation, but not in the adhesion of spores to vegetative cells. The absence of L‐BclA, which forms the L‐ENA tip fibrillum, reduced spore aggregation mediated by both S‐ENAs and L‐ENAs, highlighting the interconnected roles of S‐ENAs and L‐ENAs. We also found that increased salt concentrations in the liquid environment significantly reduced spore aggregation, suggesting a charge dependency of spore‐spore interactions. By shedding light on these complex interactions, our study offers valuable insights into spore dynamics. This knowledge can inform future studies on spore behaviour in environmental settings and assist in developing strategies to manage bacterial aggregation for beneficial purposes, such as controlling biofilms in food production equipment.
Political incentives and corporate income tax: Evidence from China
Authors: Zhao Chunxiang , Yang Yang , Huang Yong 
Published: September 2024

AbstractWe investigate how local politicians in China exploit their discretion in enforcing tax laws to enhance their prospects of promotion to higher offices. We find that non‐state‐owned enterprises (non‐SOEs) tend to pay less income tax in the initial years after a new city leader assumes office, but this tax payment increases over the leader's tenure. Throughout city leaders' tenure, the income tax paid by non‐SOEs shows an upward trend. This political turnover effect is more pronounced among non‐SOEs incorporated in the administrative region of city leaders facing intensified peer competition and/or having a stronger incentive for promotion. By manipulating the intensity of tax enforcement over their tenure, city leaders create an upward trend in tax revenue to showcase their ability to generate sustainable fiscal revenue. Further analysis indicates that growing tax revenue over the tenure significantly increases the promotion prospects of city leaders. Overall, our findings suggest that local leaders strategically manipulate tax enforcement and revenue to enhance their prospects for promotion in contemporary China.
Collective procrastination and protest cycles
Authors: Gieczewski Germán , Kocak Korhan 
Published: September 2024

AbstractThis paper studies a model of “pivotal protesting,” in which citizens act in order to change the outcome rather than to collect private benefits. We show that, when citizens face repeated opportunities to protest against a regime, pivotal protesting entails complex dynamic considerations: The continuation value of the status quo influences the citizens' willingness to protest today. Thus, a mere change in expectations about the future may trigger a revolt. The same logic often induces a pattern of protest cycles, driven by a novel source of inefficiency: An expectation that a protest will take place tomorrow can excessively sap incentives to coordinate on protesting today. Thus, potential protests crowd each other out. This can lead to a form of collective procrastination: Access to more opportunities to protest can lower the citizens' welfare, as collective action becomes inefficiently delayed.
Monitoring of myocardial injury by serial measurements of QRS area and T area: The MaastrICCht cohort
Authors: Ghossein M. A. , de Kok J. W. T. M. , Eerenberg F. , van Rosmalen F. , Boereboom R. , Duisberg F. , Verharen K. , Sels J. E. M. , Delnoij T. , Geyik Z. , Mingels A. M. A. , Meex S. J. R. , van Kuijk S. M. J. , van Stipdonk A. M. W. , Ghossein C. , Prinzen F. W. , van der Horst I. C. C. , Vernooy K. , van Bussel B. C. T. , Driessen R. G. H. 
Published: September 2024

AbstractBackgroundManually derived electrocardiographic (ECG) parameters were not associated with mortality in mechanically ventilated COVID‐19 patients in earlier studies, while increased high‐sensitivity cardiac troponin‐T (hs‐cTnT) and N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) were. To provide evidence for vectorcardiography (VCG) measures as potential cardiac monitoring tool, we investigated VCG trajectories during critical illness.MethodsAll mechanically ventilated COVID‐19 patients were included in the Maastricht Intensive Care Covid Cohort between March 2020 and October 2021. Serum hs‐cTnT and NT‐proBNP concentrations were measured daily. Conversion of daily 12‐lead ECGs to VCGs by a MATLAB‐based script provided QRS area, T area, maximal QRS amplitude, and QRS duration. Linear mixed‐effect models investigated trajectories in serum and VCG markers over time between non‐survivors and survivors, adjusted for confounders.ResultsIn 322 patients, 5461 hs‐cTnT, 5435 NT‐proBNP concentrations and 3280 ECGs and VCGs were analyzed. Non‐survivors had higher hs‐cTnT concentrations at intubation and both hs‐cTnT and NT‐proBNP significantly increased compared with survivors. In non‐survivors, the following VCG parameters decreased more when compared to survivors: QRS area (−0.27 (95% CI) (−0.37 to −0.16, p < .01) μVs per day), T area (−0.39 (−0.62 to −0.16, p < .01) μVs per day), and maximal QRS amplitude (−0.01 (−0.01 to −0.01, p < .01) mV per day). QRS duration did not differ.ConclusionVCG‐derived QRS area and T area decreased in non‐survivors compared with survivors, suggesting that an increase in myocardial damage and tissue loss play a role in the course of critical illness and may drive mortality. These VCG markers may be used to monitor critically ill patients.
Genetic variation and hybridization determine the outcomes of conservation reintroductions
Authors: Feuerstein Colter A. , Kovach Ryan P. , Kruse Carter G. , Jaeger Matt E. , Bell Donovan A. , Robinson Zachary L. , Whiteley Andrew R. 
Published: September 2024

AbstractThe preservation of genetic variation is fundamental in biodiversity conservation, yet its importance for population viability remains contentious. Mixed‐source reintroductions, where individuals are translocated into a single vacant habitat from multiple genetically divergent and often depauperate populations, provide an opportunity to evaluate how genetic variation and hybridization influence individual and relative population fitness. Population genetic theory predicts that individuals with higher genetic variation and hybrids among populations should have higher fitness. We tested these two hypotheses by analyzing individual and population‐scale data for westslope cutthroat trout (Oncorhynchus clarkii lewisi) in four mixed‐source reintroductions. We observed more hybrid and fewer nonhybrid offspring than expected across four independent mixed‐source reintroductions. We also found clear evidence that heterozygosity influenced individual reproductive and relative population fitness. Overall, we found a strong, positive relationship between genetic variation, hybridization, and transplant fitness, emphasizing the importance of genetic variation and population mixing in conservation.
Developing a comprehensive profile of professional development and identity for English language teacher educators
Authors: Ataş Ufuk , Daloğlu Ayşegül 
Published: September 2024

AbstractDespite the growing interest in researching English language teacher educators' (ELTEs) pedagogy, knowledge base, beliefs, and identity development, there is a lack of comprehensive frameworks that encompass these aspects holistically. This study attempts to develop a professional development profile for English language teacher educators by investigating how they define their profession, what professional characteristics and personality traits they possess, what roles and responsibilities they assume, and what constitutes the domains of a knowledge base. Additionally, it explores how they perceive their identities as educators and engage in professional development. The participants were 84 English language teacher educators from across Türkiye. Data were collected via face‐to‐face semi‐structured interviews and analysed thematically. At the end of this study, an integrated professional profile of English language teacher educators is provided, which includes insights into their professional identities. The profile has implications beyond Türkiye and might contribute to the broader understanding and enhancement of professional development for language teacher educators globally. As the study addresses a gap in the field, its outcomes are expected to contribute to the ongoing discussions and efforts to support teacher educators in their continuous learning and development.