bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementing open science and reproducible research practices in mental health research through registered reports
Authors: Baldwin Jessie R. , Larsson Henrik 
Published: September 2024

AbstractTo increase the number of pre‐registered observational studies, Journal of Child Psychology and Psychiatry (JCPP) Advances is delighted to now invite Registered Reports. Registered Reports are a format of article in which the study protocol is pre‐registered and peer‐reviewed before the research is conducted. If the protocol is of high quality and the proposed research topic is important, JCPP Advances will commit to publishing the study regardless of the results. This article format crucially addresses publication bias, as decisions on publication are entirely independent of the results.
N121T and N121S substitutions on the SARS‐CoV‐2 spike protein impact on serum neutralization
Authors: Lo Van Thi , Lim Hyun A. , Jang Seong Sik , Kim Min Chan , Chamfort Alain Chrysler , Kim Ha Yeon , Mun Da Young , Kang Min Chang , Lee Han Byul , Kim Sunjoo , Lee Younghee , Park Sangkyu , Yoon Sun‐Woo , Kim Hye Kwon 
Published: September 2024

AbstractThe N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment. Although N121T and N121S substitutions are not currently major SARS‐CoV‐2 variants of concern, this study could provide fundamental information to prepare for potential future mutations at the N121 site of SARS‐CoV‐2.
Criteria for second generation comparator tests in validation of novel HPV DNA tests for use in cervical cancer screening
Authors: Arbyn Marc , Cuschieri Kate , Bonde Jesper , Schuurman Rob , Cocuzza Clementina , Broeck Davy Vanden , Zhao Fang‐Hui , Rezhake Remila , Gultekin Murat , de Sanjosé Silvia , Canfell Karen , Hawkes David , Saville Marion , Hillemanns Peter , Dillner Joakim , Berkhof Johannes , Prétet Jean‐Luc , Gheit Tarik , Clifford Gary , Basu Partha , Almonte Maribel , Wentzensen Nicolas , Poljak Mario 
Published: September 2024

AbstractWhile HC2 and GP5+/6+ PCR‐EIA were pivotal in test validation of new HPV assays, they represent the first generation of comparator tests based upon technologies that are not in widespread use anymore. In the current guideline, criteria for second‐generation comparator tests are presented that include more detailed resolution of HPV genotypes. Second‐generation comparator tests should preferentially target only the 12 genotypes classified as carcinogenic (IARC‐group I), and show consistent non‐inferior sensitivity for CIN2+ and CIN3+ and specificity for ≤CIN1 compared to one of the first‐generations comparators, in at least three validation studies using benchmarks of 0.95 for relative sensitivity and 0.98 for relative specificity. Validation should take into account used storage media and other sample handling procedures. Meta‐analyses were conducted to identify the assays that fulfill these stringent criteria. Four tests fulfilled the new criteria: (1) RealTime High‐Risk HPV Test (Abbott), (2) Cobas‐4800 HPV test (Roche Molecular System), (3) Onclarity HPV Assay (BD Diagnostics), and (4) Anyplex II HPV HR Detection (Seegene), each evaluated in three to six studies. Whereas the four assays target 14 carcinogenic genotypes, the first two identify separately HPV16 and 18, the third assay identifies five types separately and the fourth identifies all the types separately.
Serial transverse enteroplasty in adults with parenteral nutrition dependence: A case series
Authors: Mlaver Eli , Smith Savannah R. , Matar Abraham J. , Zhao Vivian M. , Leong Rachel , Sharma Jyotirmay , Srinivasan Jahnavi K. , Galloway John R. 
Published: September 2024

AbstractBackgroundSerial transverse enteroplasty is used to treat patients with chronic intestinal failure owing to short bowel syndrome. Current literature lacks discussion of its role for other etiologies of intestinal failure and its impact on adult patients' nutrition support needs and quality of life.MethodsWe performed a case series on adults with parenteral nutrition (PN) dependence who underwent serial transverse enteroplasty at Emory University Hospital, a quaternary referral center between 2011 and 2022. Data collected included demographics, operative technique, and preoperative and postoperative PN requirements. A phone survey was administered to evaluate the impact of PN and the operation on quality of life.ResultsTen patients underwent the procedure of interest during the study period. Indications included short bowel syndrome following multiple abdominal operations or intra‐abdominal catastrophe and chronic partial bowel obstruction with dysmotility. Bowel length increased by a median of 83%. All patients were discharged home after a median hospital stay of 21 days. At 1‐year follow‐up, survival was 100%, two (20%) patients fully weaned from PN, three others (30%) reduced PN frequency, and six (60%) decreased their daily parenteral energy requirement. Two additional patients fully weaned from PN by 18 months postoperatively.ConclusionThis represents one of the largest case series of serial transverse enteroplasty in adults. Small intestinal length nearly doubled, and PN dependence was reduced in most patients. Given the low morbidity and good quality of life observed in this series, this procedure should be more widely investigated for patients with chronic intestinal failure.
A Novel Prediction Model for Churning Power Loss of Spur Gear
Authors: Jia Fuchun , Wang Binyu , Fu Yao 
Published: September 2024

ABSTRACTThe prediction of churning power loss has been a difficult problem in the analysis of spur gears. Thus, an analytical prediction model based on the redefinition of churning power loss and energy transformation is proposed to estimate the churning power of spur gears. Churning power loss is defined as the combination of the power loss due to the drag on the end face, the power loss due to the tangential flow, the power loss due to the acceleration of lubricants in the tooth space, and the power loss due to the centrifugal force. Several comparisons of prediction and experimental results are made and good agreement of those is obtained. Finally, the components of churning power loss under different gears and work conditions are analyzed, and the influences of each part on churning power loss are obtained.
On the stability of constant higher order mean curvature hypersurfaces in a Riemannian manifold
Authors: Elbert Maria Fernanda , Nelli Barbara 
Published: September 2024

AbstractWe propose a notion of stability for constant ‐mean curvature hypersurfaces in a general Riemannian manifold and we give some applications. When the ambient manifold is a Space Form, our notion coincides with the known one, given by means of the variational problem.
Posttransplant complications: molecular mechanisms and therapeutic interventions
Authors: Liu Xiaoyou , Shen Junyi , Yan Hongyan , Hu Jianmin , Liao Guorong , Liu Ding , Zhou Song , Zhang Jie , Liao Jun , Guo Zefeng , Li Yuzhu , Yang Siqiang , Li Shichao , Chen Hua , Guo Ying , Li Min , Fan Lipei , Li Liuyang , Luo Peng , Zhao Ming , Liu Yongguang 
Published: September 2024

AbstractPosttransplantation complications pose a major challenge to the long‐term survival and quality of life of organ transplant recipients. These complications encompass immune‐mediated complications, infectious complications, metabolic complications, and malignancies, with each type influenced by various risk factors and pathological mechanisms. The molecular mechanisms underlying posttransplantation complications involve a complex interplay of immunological, metabolic, and oncogenic processes, including innate and adaptive immune activation, immunosuppressant side effects, and viral reactivation. Here, we provide a comprehensive overview of the clinical features, risk factors, and molecular mechanisms of major posttransplantation complications. We systematically summarize the current understanding of the immunological basis of allograft rejection and graft‐versus‐host disease, the metabolic dysregulation associated with immunosuppressive agents, and the role of oncogenic viruses in posttransplantation malignancies. Furthermore, we discuss potential prevention and intervention strategies based on these mechanistic insights, highlighting the importance of optimizing immunosuppressive regimens, enhancing infection prophylaxis, and implementing targeted therapies. We also emphasize the need for future research to develop individualized complication control strategies under the guidance of precision medicine, ultimately improving the prognosis and quality of life of transplant recipients.
Targeting chronic lymphocytic leukemia with B‐cell activating factor receptor CAR T cells
Authors: Qie Yaqing , Gadd Martha E. , Shao Qing , To Tommy , Liu Andrew , Li Shuhua , Rivera‐Valentin Rocio , Yassine Farah , Murthy Hemant S. , Dronca Roxana , Kharfan‐Dabaja Mohamed A. , Qin Hong , Luo Yan 
Published: September 2024

AbstractThe challenge of disease relapsed/refractory (R/R) remains a therapeutic hurdle in chimeric antigen receptor (CAR) T‐cell therapy, especially for hematological diseases, with chronic lymphocytic leukemia (CLL) being particularly resistant to CD19 CAR T cells. Currently, there is no approved CAR T‐cell therapy for CLL patients. In this study, we aimed to address this unmet medical need by choosing the B‐cell activating factor receptor (BAFF‐R) as a promising target for CAR design against CLL. BAFF‐R is essential for B‐cell survival and is consistently expressed on CLL tumors. Our research discovered that BAFF‐R CAR T‐cell therapy exerted the cytotoxic effects on both CLL cell lines and primary B cells derived from CLL patients. In addition, the CAR T cells exhibited cytotoxicity against CD19‐knockout CLL cells that are resistant to CD19 CAR T therapy. Furthermore, we were able to generate BAFF‐R CAR T cells from small blood samples collected from CLL patients and then demonstrated the cytotoxic effects of these patient‐derived CAR T cells against autologous tumor cells. Given these promising results, BAFF‐R CAR T‐cell therapy has the potential to meet the long‐standing need for an effective treatment on CLL patients.
Targeting RAC1 reactivates pyroptosis to reverse paclitaxel resistance in ovarian cancer by suppressing P21‐activated kinase 4
Authors: Wu Jiangchun , Wu Yong , Zhao Tianyi , Wang Xiangwei , Guo Qinhao , Wang Simin , Chen Siyu , Ju Xingzhu , Li Jin , Wu Xiaohua , Zheng Zhong 
Published: September 2024

AbstractPyroptosis may play an important role in the resistance of ovarian cancer (OC) to chemotherapy. However, the mechanism by which pyroptosis modulation can attenuate chemotherapy resistance has not been comprehensively studied in OC. Here, we demonstrated that RAS‐associated C3 botulinum toxin substrate 1 (RAC1) is highly expressed in OC and is negatively correlated with patient outcomes. Through cell function tests and in vivo tumor formation tests, we found that RAC1 can promote tumor growth by mediating paclitaxel (PTX) resistance. RAC1 can mediate OC progression by inhibiting pyroptosis, as evidenced by high‐throughput automated confocal imaging, the release of lactate dehydrogenase (LDH), the expression of the inflammatory cytokines IL‐1β/IL‐18 and the nucleotide oligomerization domain‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome. Mechanically, RNA‐seq, gene set enrichment analysis (GSEA), coimmunoprecipitation (Co‐IP), mass spectrometry (MS), and ubiquitination tests further confirmed that RAC1 inhibits caspase‐1/gasdermin D (GSDMD)‐mediated canonical pyroptosis through the P21‐activated kinase 4 (PAK4)/mitogen‐activated protein kinase (MAPK) pathway, thereby promoting PTX resistance in OC cells. Finally, the whole molecular pathway was verified by the results of in vivo drug combination tests, clinical specimen detection and the prognosis. In summary, our results suggest that the combination of RAC1 inhibitors with PTX can reverse PTX resistance by inducing pyroptosis through the PAK4/MAPK pathway.
Fast, automated, real‐time 3D passive balloon catheter tracking during MRI‐guided cardiac catheterization using orthogonal projection imaging and real‐time image‐based catheter detection
Authors: Kowalik Grzegorz T. , Kerfoot Eric , Kunze Karl , Neji Radhouene , Moon Tracy , Mellor Nina , Razavi Reza , Pushparajah Kuberan , Roujol Sébastien 
Published: September 2024

AbstractPurposeMRI‐guidance of cardiac catheterization is currently performed using one or multiple 2D imaging planes, which may be suboptimal for catheter navigation, especially in patients with complex anatomies. The purpose of the work was to develop a robust real‐time 3D catheter tracking method and 3D visualization strategy for improved MRI‐guidance of cardiac catheterization procedures.MethodsA fast 3D tracking technique was developed using continuous acquisition of two orthogonal 2D‐projection images. Each projection corresponds to a gradient echo stack of slices with only the central k‐space lines being collected for each slice. To enhance catheter contrast, a saturation pulse is added ahead of the projection pair. An offline image processing algorithm was developed to identify the 2D coordinates of the balloon in each projection image and to estimate its corresponding 3D coordinates. Post‐processing includes background signal suppression using an atlas of background 2D‐projection images. 3D visualization of the catheter and anatomy is proposed using three live sagittal, coronal, and axial (MPR) views and 3D rendering. The technique was tested in a subset of a catheterization step in three patients undergoing MRI‐guided cardiac catheterization using a passive balloon catheter.ResultsThe extraction of the catheter balloon 3D coordinates was successful in all patients and for the majority of time‐points (accuracy >96%). This tracking method enabled a novel 3D visualization strategy for passive balloon catheter, providing enhanced anatomical context during catheter navigation.ConclusionThe proposed tracking strategy shows promise for robust tracking of passive balloon catheter and may enable enhanced visualization during MRI‐guided cardiac catheterization.
High‐quality FLORET UTE imaging for clinical translation
Authors: Willmering Matthew M. , Krishnamoorthy Guruprasad , Robison Ryan K. , Rosenberg Jens T. , Woods Jason C. , Pipe James G. 
Published: September 2024

AbstractPurposeTo develop a robust 3D ultrashort‐TE (UTE) protocol that can reproducibly provide high‐quality images, assessed by the ability to yield clinically diagnostic images, and is suitable for clinical translation.Theory and MethodsBuilding on previous work, a UTE sampled with Fermat looped orthogonally encoded trajectories (FLORET) was chosen as a starting point due to its shorter, clinically reasonable scan times. Modifications to previous FLORET implementations included gradient waveform frequency limitations, a new trajectory ordering scheme, a balanced SSFP implementation, fast gradient spoiling, and full inline reconstruction. FLORET images were collected in phantoms and humans on multiple scanners and sites to demonstrate these improvements.ResultsThe updates to FLORET provided high‐quality images in phantom, musculoskeletal, and pulmonary applications. The gradient waveform modifications and new trajectory ordering scheme significantly reduced visible artifacts. Fast spoiling reduced acquisition time by 20%–28%. Across the various scanners and sites, the inline image quality was consistent and of diagnostic quality. Total image acquisition plus reconstruction time was less than 4 min for musculoskeletal and pulmonary applications with reconstructions taking less than 1 min.ConclusionRecently developed improvements for the FLORET sequence have enabled robust, high‐quality UTE acquisitions with short acquisition and reconstruction times. This enables clinical UTE imaging as demonstrated by the implementation of the sequence and acquisition on five MRI scanners, at three different sites, without the need for any additional system characterization or measurements.
Skeletal muscle symptoms and quantitative MRI in females with dystrophinopathy
Authors: Jenkins Breana M. , Dixon Lathan D. , Kokesh Kevin J. , Zingariello Carla D. , Vandenborne Krista , Walter Glenn A. , Barnard Alison M. 
Published: September 2024

AbstractIntroduction/AimsThe dystrophinopathies primarily affect males; however, female carriers of pathogenic dystrophin variants can develop skeletal muscle symptoms. This study aimed to evaluate muscle involvement and symptoms in females with dystrophinopathy using quantitative magnetic resonance imaging (MRI), functional assessments, and patient‐reported outcomes.MethodsControls and females with dystrophinopathy with muscle symptoms of pain, weakness, fatigue, or excessive tightness were enrolled in this cross‐sectional study. Participants underwent lower extremity MRI to quantify muscle inflammation, replacement by fat, and disease asymmetry. Cardiac MRI, functional ability, muscle symptoms, and serum creatine kinase levels were also evaluated.ResultsSix pediatric females with dystrophinopathy (mean age: 11.7 years), 11 adult females with dystrophinopathy (mean age: 41.3 years), and seven controls enrolled. The mean fat fraction was increased in females with dystrophinopathy compared to controls in the soleus (0.11 vs. 0.03, p = .0272) and vastus lateralis (0.16 vs. 0.03, p = .004). Magnetic resonance spectroscopy water T2, indicative of muscle inflammation, was elevated in the soleus and/or vastus lateralis in 11 of 17 individuals. North Star Ambulatory Assessment score was lower in the dystrophinopathy group compared to controls (29 vs. 34 points, p = .0428). From cardiac MRI, left ventricle T1 relaxation times were elevated in females with dystrophinopathy compared to controls (1311 ± 55 vs. 1263 ± 25 ms, p < .05), but ejection fraction and circumferential strain did not differ.DiscussionSymptomatic females with dystrophinopathy quantitatively demonstrate muscle replacement by fat and inflammation, along with impairments in functional ability and cardiac function. Additional research is needed to evaluate how symptoms and muscle involvement change longitudinally.
Giving Journeys: The Role of Private Foundations
Authors: Williamson Alexandra , Luke Belinda 
Published: September 2024

ABSTRACTThe giving journeys of individuals, relating to their life stories and life places, have been positioned in the literature as romanticized versions of self. This research adopts a theory of philanthropic identity narratives using a journey metaphor, and explores the role and journeys associated with establishing a giving structure in the form of a private foundation. Findings from interviews with managers and trustees of private philanthropic foundations undertaken in two phases, 7 years apart, show that establishing a foundation shifts the emphasis of a giving journey from being individual‐focused to philanthropy‐focused, facilitating a legacy of giving rather than self. This paper contributes to theoretical and practical understandings of philanthropic journeys by adding a foundation lens, highlighting the shift in emphasis between identity and action, where establishing a giving structure is less about an individual founder's identity and more about focused philanthropic activity, irrespective of what that particular focus may be.
Feasibility of using urinary c‐peptide to guide diabetes management in a district general hospital type 1 diabetes population
Authors: Redford Christopher 
Published: September 2024

AbstractBackground: C‐peptide measurement is a well‐recognised tool for distinguishing individuals with insulin deficiency from those with retained insulin secretion; however, it is not routinely performed across the UK.Aims: This study aimed to evaluate the feasibility and efficacy of routine urinary c‐peptide testing in a district general hospital type 1 diabetes clinic.Methods: In this single‐centre study, adults with type 1 diabetes for at least 3 years were offered random urine c‐peptide–to–creatinine ratio (UCPCR) testing during routine clinic review. A UCPCR of >0.2 prompted further evaluation of the individual and their diabetes treatment.Results: Of 200 recruited individuals, 172 (87%) submitted a urine sample. Of the 172 individuals, 26 (15%) had a UCPCR of >0.2, which prompted a review of their diagnosis and treatment strategies, and 12 (7%) had a UCPCR of >0.6, suggesting significant inherent insulin secretion and a likelihood of positive response to alternative or additional pharmacological agents.Conclusions: Urinary c‐peptide level testing performed routinely at a district general hospital type 1 diabetes clinic is practical. It can alter patient clinical outcomes, lead to more appropriate and effective pharmacological treatments and ensure appropriate use of expensive technologies.
The role of protein corona in advancing plasma proteomics
Authors: Saei Amir Ata , Sun Liangliang , Mahmoudi Morteza 
Published: September 2024

AbstractThe protein corona, a layer of biomolecules forming around nanoparticles in biological environments, critically influences nanoparticle interactions with biosystems, affecting pharmacokinetics and biological outcomes. Initially, the protein corona presented challenges for nanomedicine and nanotoxicology, such as nutrient depletion in cell cultures and masking of nanoparticle‐targeting species. However, recent advancements have highlighted its potential in environmental toxicity, proteomics, and immunology. This viewpoint focuses on leveraging the protein corona to enhance the depth of plasma proteome analysis, addressing challenges posed by the high dynamic range of protein concentrations in plasma. The protein corona simplifies sample preparation, enriches low‐abundance proteins, and improves proteome coverage. Innovations include using diverse nanoparticles and spiking small molecules to increase the number of quantified proteins. Reproducibility issues across core facilities necessitate standardized protocols. Moreover, top‐down proteomics enables proteoform‐specific measurements, providing deeper insights into protein corona composition. Future research should aim at improving top‐down proteomics techniques and integrating protein corona studies and proteomics for personalized medicine and advanced diagnostics.