bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of functional interaction between potassium channel Kv1.3 and sodium channel NavBeta1 subunit
Authors: Kubota Tomoya , Correa Ana M. , Bezanilla Francisco 
Published: March 2017

AbstractThe voltage-gated potassium channel subfamily A member 3 (Kv1.3) dominantly expresses on T cells and neurons. Recently, the interaction between Kv1.3 and NavBeta1 subunits has been explored through ionic current measurements, but the molecular mechanism has not been elucidated yet. We explored the functional interaction between Kv1.3 and NavBeta1 through gating current measurements using the Cut-open Oocyte Voltage Clamp (COVC) technique. We showed that the N-terminal 1–52 sequence of hKv1.3 disrupts the channel expression on theXenopusoocyte membrane, suggesting a potential role as regulator of hKv1.3 expression in neurons and lymphocytes. Our gating currents measurements showed that NavBeta1 interacts with the voltage sensing domain (VSD) of Kv1.3 through W172 in the transmembrane segment and modifies the gating operation. The comparison between G-V and Q-V with/without NavBeta1 indicates that NavBeta1 may strengthen the coupling between hKv1.3-VSD movement and pore opening, inducing the modification of kinetics in ionic activation and deactivation.
Strain-specific estimation of epidemic success provides insights into the transmission dynamics of tuberculosis
Authors: Rasigade Jean-Philippe , Barbier Maxime , Dumitrescu Oana , Pichat Catherine , Carret Gérard , Ronnaux-Baron Anne-Sophie , Blasquez Ghislaine , Godin-Benhaim Christine , Boisset Sandrine , Carricajo Anne , Jacomo Véronique , Fredenucci Isabelle , Pérouse de Montclos Michèle , Flandrois Jean-Pierre , Ader Florence , Supply Philip , Lina Gérard , Wirth Thierry 
Published: March 2017

AbstractThe transmission dynamics of tuberculosis involves complex interactions of socio-economic and, possibly, microbiological factors. We describe an analytical framework to infer factors of epidemic success based on the joint analysis of epidemiological, clinical and pathogen genetic data. We derive isolate-specific, genetic distance-based estimates of epidemic success, and we represent success-related time-dependent concepts, namely epidemicity and endemicity, by restricting analysis to specific time scales. The method is applied to analyze a surveillance-based cohort of 1,641 tuberculosis patients with minisatellite-based isolate genotypes. Known predictors of isolate endemicity (older age, native status) and epidemicity (younger age, sputum smear positivity) were identified with high confidence (P < 0.001). Long-term epidemic success also correlated with the ability of Euro-American and Beijing MTBC lineages to cause active pulmonary infection, independent of patient age and country of origin. Our results demonstrate how important insights into the transmission dynamics of tuberculosis can be gained from active surveillance data.
Proteome dynamics during postnatal mouse corpus callosum development
Authors: Son Alexander I. , Fu Xiaoqin , Suto Fumikazu , Liu Judy S. , Hashimoto-Torii Kazue , Torii Masaaki 
Published: March 2017

AbstractFormation of cortical connections requires the precise coordination of numerous discrete phases. This is particularly significant with regard to the corpus callosum, whose development undergoes several dynamic stages including the crossing of axon projections, elimination of exuberant projections, and myelination of established tracts. To comprehensively characterize the molecular events in this dynamic process, we set to determine the distinct temporal expression of proteins regulating the formation of the corpus callosum and their respective developmental functions. Mass spectrometry-based proteomic profiling was performed on early postnatal mouse corpus callosi, for which limited evidence has been obtained previously, using stable isotope of labeled amino acids in mammals (SILAM). The analyzed corpus callosi had distinct proteomic profiles depending on age, indicating rapid progression of specific molecular events during this period. The proteomic profiles were then segregated into five separate clusters, each with distinct trajectories relevant to their intended developmental functions. Our analysis both confirms many previously-identified proteins in aspects of corpus callosum development, and identifies new candidates in understudied areas of development including callosal axon refinement. We present a valuable resource for identifying new proteins integral to corpus callosum development that will provide new insights into the development and diseases afflicting this structure.
Silver Nanowires Modified with PEDOT: PSS and Graphene for Organic Light-Emitting Diodes Anode
Authors: Xu Yilin , Wei Xiang , Wang Cong , Cao Jin , Chen Yigang , Ma Zhongquan , You Ying , Wan Jixiang , Fang Xiaohong , Chen Xiaoyuan 
Published: March 2017

AbstractSilver nanowires (AgNWs) networks are promising candidates for the replacement of indium tin oxide (ITO). However, the surface roughness of the AgNWs network is still too high for its application in optoelectronic devices. In this work, we have reduced the surface roughness of the AgNWs networks to 6.4 nm, compared to 33.9 nm of the as-deposited AgNWs network through the hot-pressing process, treatment with poly (3,4ethylenedioxythiophene)–poly (styrenesulfanate), and covered with graphene films. Using this method, we are able to produce AgNWs/PEDOT: PSS/SLG composite films with the transmittance and sheet resistance of 88.29% and 30 Ω/□, respectively. The OLEDs based on the AgNWs/PEDOT: PSS/SLG anodes are comparable to those based on ITO anodes.
Conditional Deletion of the Phd2 Gene in Articular Chondrocytes Accelerates Differentiation and Reduces Articular Cartilage Thickness
Authors: Cheng Shaohong , Pourteymoor Sheila , Alarcon Catrina , Mohan Subburaman 
Published: March 2017

AbstractBased on our findings that PHD2 is a negative regulator of chondrocyte differentiation and that hypoxia signaling is implicated in the pathogenesis of osteoarthritis, we investigated the consequence of disruption of thePhd2gene in chondrocytes on the articular cartilage phenotype in mice. Immunohistochemistry detected high expression of PHD2 in the superficial zone (SZ), while PHD3 and HIF-1α (target of PHD2) are mainly expressed in the middle-deep zone (MDZ). Conditional deletion of thePhd2gene (cKO) in chondrocytes accelerated the transition of progenitors to hypertrophic (differentiating) chondrocytes as revealed by reduced SZ thickness, and increased MDZ thickness, as well as increased chondrocyte hypertrophy. Immunohistochemistry further revealed decreased levels of progenitor markers but increased levels of hypertrophy markers in the articular cartilage of the cKO mice. Treatment of primary articular chondrocytes,in vitro, with IOX2, a specific inhibitor of PHD2, promoted articular chondrocyte differentiation. Knockdown ofHif-1αexpression in primary articular chondrocytes using lentiviral vectors containingHif-1αshRNA resulted in reduced expression levels ofVegf, Glut1, Pgk1, andCol10compared to control shRNA. We conclude thatPhd2is a key regulator of articular cartilage development that acts by inhibiting the differentiation of articular cartilage progenitors via modulating HIF-1α signaling.
Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates
Authors: Pinto Tatiana C. A. , Costa Natalia S. , Castro Luciana F. S. , Ribeiro Rachel L. , Botelho Ana Caroline N. , Neves Felipe P. G. , Peralta Jose Mauro , Teixeira Lucia M. 
Published: March 2017

AbstractStreptococcus pneumoniae can be classified in more than 90 capsular types, as traditionally determined by serological methods and more recently by PCR-based techniques. Such methods, however, can be expensive, laborious or unable to accurately discriminate among certain serotypes. Therefore, determination of capsular types, although extremely important for epidemiological purposes and for estimating the impact of pneumococcal conjugate vaccines, is mainly restricted to research laboratories, being rarely performed in the clinical setting. In the present study, MALDI-TOF MS was evaluated as an alternative tool to characterize 416 pneumococcal isolates belonging to serotypes 6A, 6B, 6C, 9N, 9V or 14. For MALDI-TOF MS analysis, each isolate was submitted to an extraction protocol using formic acid and acetonitrile. Measurements were performed with a Bruker Microflex LT mass spectrometer using default parameters and generating spectra in the range of 2,000–20,000 m/z. Spectra were analyzed with the BioNumerics software v7.6. Isolates were mainly distributed according to the capsular type in a Neighbor Joining tree and serotypes investigated were successfully discriminated by the presence/absence of 14 selected biomarkers. The results suggest that MALDI-TOF MS is a promising alternative for typing pneumococcal strains, highlighting its usefulness for rapid and cost-effective routine application in clinical laboratories.
Final report of APMP.QM-K46 ammonia in nitrogen at 30 μmol/mol level
Authors: Uehara Shinji , Qiao Han , Shimosaka Takuya 
Published: March 2017

This report describes the results for a bilateral comparison of ammonia in nitrogen gas mixture. The nominal amount-of-substance fraction was 30 μmol/mol.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Association Between Hospital Case Volume of Sepsis, Adherence to Evidence-Based Processes of Care and Patient Outcomes
Authors: Fawzy Ashraf , Walkey Allan J. 
Published: March 2017

Objectives:
We sought to explore potential mechanisms underlying hospital sepsis case volume-mortality associations by investigating implementation of evidence-based processes of care.

Design:
Retrospective cohort study. We determined associations of sepsis case volume with three evidence-based processes of care (lactate measurement during first hospital day, norepinephrine as first vasopressor, and avoidance of starch-based colloids) and assessed their role in mediation of case volume-mortality associations.

Setting:
Enhanced administrative data (Premier, Charlotte, NC) from 534 U.S. hospitals.

Subjects:
A total of 287,914 adult patients with sepsis present at admission between July 2010 and December 2012 of whom 58,045 received a vasopressor for septic shock during the first 2 days of hospitalization.

Interventions:
None.

Measurements and Main Results:
Among patients with sepsis, 1.9% received starch, and among patients with septic shock, 68.3% had lactate measured and 64% received norepinephrine as initial vasopressor. Patients at hospitals with the highest case volume were more likely to have lactate measured (adjusted odds ratio quartile 4 vs quartile 1, 2.8; 95% CI, 2.1–3.7) and receive norepinephrine as initial vasopressor (adjusted odds ratio quartile 4 vs quartile 1, 2.1; 95% CI, 1.6–2.7). Case volume was not associated with avoidance of starch products (adjusted odds ratio quartile 4 vs quartile 1, 0.73; 95% CI, 0.45–1.2). Adherence to evidence-based care was associated with lower hospital mortality (adjusted odds ratio, 0.81; 95% CI, 0.70–0.94) but did not strongly mediate case volume-mortality associations (point estimate change ≤ 2%).

Conclusions:
In a large cohort of U.S. patients with sepsis, select evidence-based processes of care were more likely implemented at high-volume hospitals but did not strongly mediate case volume-mortality associations. Considering processes and case volume when regionalizing sepsis care may maximize patient outcomes.
Reasons for Interfacility Emergency Department Transfer and Care at the Receiving Facility
Authors: Li Joyce , Pryor Stephanie , Choi Ben , Rees Chris A. , Senthil Mamata V. , Tsarouhas Nicholas , Myers Sage R. , Monuteaux Michael C. , Bachur Richard G. 
Published: March 2017

Objectives
The aims of this study were to (1) assess the reasons for pediatric interfacility transfers as identified by transferring providers and review the emergency medical care delivered at the receiving facilities and (2) investigate the emergency department (ED) care among the subpopulation of patients discharged from the receiving facility.

Methods
We performed a multicenter, cross-sectional survey of ED medical providers transferring patients younger than 18 years to 1 of 4 US tertiary care pediatric hospitals with a subsequent medical record review at the receiving facility. Referring providers completed surveys detailing reasons for transfer.

Results
Eight hundred thirty-nine surveys were completed by 641 providers for 25 months. The median patient age was 5.7 years. Sixty-two percent of the patients required admission. The most common reasons for transfer as cited by referring providers were subspecialist consultation (62%) and admission to a pediatric inpatient (17%) or intensive care (6%) unit. For discharged patients, plain radiography (26%) and ultrasonography (12%) were the most common radiologic studies. Procedural sedation (16%) was the most common ED procedure for discharged patients, and 55% had a subspecialist consult at the receiving facility. Ten percent of interfacility transfers did not require subspecialty consult, ED procedure, radiologic study, or admission.

Conclusions
Approximately 4 of 10 interfacility transfers are discharged by the receiving facility, suggesting an opportunity to provide more comprehensive care at referring facilities. On the basis of the care provided at the receiving facility, potential interventions might include increased subspecialty access and developing both ultrasound and sedation capabilities.
Validity of an Accelerometer Used to Measure Step Count in Children With Idiopathic Toe Walking
Authors: Christensen Catie , Haddad Amanda , Maus Elizabeth 
Published: March 2017

Purpose:
To assess the validity of accelerometer use in children with idiopathic toe walking (ITW).

Methods:
Seventy-five children, 2 to 13 years old, with ITW were videotaped ambulating 50 ft wearing a NL-1000 accelerometer. Because of concerns for accelerometer accuracy in young children, 2 groups were created: 5 years or younger (n = 45) and 6 years or older (n = 30). Step counts recorded via accelerometer and video were compared and correlated.

Results:
There was a significant difference in accelerometer and video step counts for 2- to 5-year-olds (W = 72.00, P < .001). No significant difference was found for 6- to 13-year-olds (W = 65.00, P = .24). Spearman ρ values were 0.78 and 0.92 for the 5 years or younger and 6 years or older groups, respectively.

Conclusion:
Accelerometers accurately count steps in 6- to 13-year-olds with ITW but are inaccurate in 2- to 5-year-olds.
Corrosion performance of aluminium in atmospheric, underground and seawater splatter zone in the northeastern coast of Arabian Peninsula
Authors: Ul-Hamid Anwar , Al-Hems Luai M. , Quddus Abdul , Muhammed Abdulrashid I. , Saricimen Huseyin 
Published: March 2017

Purpose
The purpose of this study was to determine the atmospheric corrosion behavior of aluminium (Al) exposed to the industrial and coastal environments of northeastern Arabian Peninsula for a period of 15 months.

Design/methodology/approach
The samples were exposed under atmospheric, underground and splatter zone conditions at the coastal region. Soil, groundwater, seawater and air particulate samples obtained from the exposure site were analyzed. Secondary electron microscopy was used to identify and study the microstructural features of the corrosion products formed at the surface of the test coupons. The corrosion rates of the samples were determined by the weight loss method.

Findings
The results showed that Al exhibited a moderate corrosion rate despite high degree of variation in temperature and humidity and large concentrations of chloride and sulfate in this region. Splatter zone environment was the most corrosive because of high chloride concentrations in seawater and the alternating wetting–drying cycles.

Originality/value
In this paper, corrosion of Al was evaluated in atmospheric, soil and splatter zone conditions along the northeastern coast of Arabian Peninsula and was also compared with the results of the test reported for other international locations.
Conjugate rotor-stator interaction procedure for film-cooled turbine sections
Authors: Gottlieb Joshua , Davis Roger , Clark John 
Published: March 2017

Purpose
The authors aim to present a procedure for the parallel, steady and unsteady conjugate, Navier–Stokes/heat-conduction rotor-stator interaction analysis of multi-blade-row, film-cooled, turbine airfoil sections. A new grid generation procedure for multiple blade-row configurations, including walls, thermal barrier coatings, plenums, and cooling tubes, is discussed.

Design/methodology/approach
Steady, multi-blade-row interaction effects on the flow and wall thermal fields are predicted using a Reynolds’s-averaged Navier–Stokes (RANS) simulation in conjunction with an inter-blade-row mixing plane. Unsteady, aero-thermal interaction solutions are determined using time-accurate sliding grids between the stator and rotor with an unsteady RANS model. Non-reflecting boundary condition treatments are utilized in both steady and unsteady approaches at all inlet, exit and inter-blade-row boundaries. Parallelization techniques are also discussed.

Findings
The procedures developed in this research are compared against experimental data from the Air Force Research Laboratory’s turbine research facility.

Practical implications
The software presented in this paper is useful as both the design and analysis tool for fluid system and turbomachinery engineers.

Originality/value
This research presents a novel approach for the simultaneous solution of fluid flow and heat transfer in film-cooled rotating turbine sections. The software developed in this research is validated against experimental results for 2D flow, and the methods discussed are extendable to 3D.
Does involvement shapes consumers’ response to product failure?
Authors: Hamzelu Bahman , Gohary Ali , Ghafoori Nia Salar , Heidarzadeh Hanzaee Kambiz 
Published: March 2017

Purpose
Customer reaction to failure is of essential importance and varies by level of involvement with products and services. Thus, the purpose of this paper is to use the FCB grid to examine effects of involvement and emotion on failure of products and services. It also explores effects of negative word-of-mouth, consumer advocacy, customer voicing and gender on the so-called silent killers.

Design/methodology/approach
A 2 (male or female)×4 (high and low involvement, feelings, thinking in FCB grid) between-subjects experiment on 311 college students, who have recently experienced product failure, is performed.

Findings
Results reveal that customers with different levels of involvement react differently to product failure. Furthermore, low-involvement products are more likely to develop silent killers. The results also show that silent killer is more common among men.

Originality/value
To the best of the authors’ knowledge, no similar study is performed on the relationship between involvement and failure of products or services. In addition, this attempt is the first quantitative study to examine the phenomenon of silent killers in this field.
Critical crossroads to explain network change: evidence from a goal-directed network
Authors: Agostino Deborah , Arnaboldi Michela , Dal Molin Martina 
Published: March 2017

Purpose
Public networks studies have widely diffused in recent years, but scant attention has been devoted to network change. By endorsing the notion of critical crossroads to describe a crucial turning point for the network survival, the purpose of this paper is to investigate how and why a goal-directed network changes, considering both the benefits and the constraints of the change.

Design/methodology/approach
This study adopts a longitudinal case study based upon an interventionist research approach (Jönsson and Lukka, 2006), with the researchers being immersed in the network life of a group of Italian public universities over a period of 17 years.

Findings
This paper proposes an empirical derived framework about network evolution that identifies two different types of crossroads (i.e. resource-driven crossroads and management driven) as drivers for network evolution. The main determinant behind these crisis situation were found in the heterogeneity of the network actors and, while overcoming the crossroads, informal sub-networks were found emerging.

Originality/value
This study enlarges current public network literature by focusing specifically on how and why networks change, an aspect underinvestigated by current literature.
Attractiveness of the United States as a travel destination for the Russian tourist in the era of strained bilateral relations
Authors: Stepchenkova Svetlana , Shichkova Elena 
Published: March 2017

Purpose
The purpose of this paper is to examine the attractiveness of a global tourism destination when the country of that destination and the country that provides the source market are in a situation of political and economic conflict. In this study, the USA is the vacation destination, and Russia is the tourism generating region.

Design/methodology/approach
In May 2015, 18 Russian tourists from a large regional urban center participated in qualitative semi-structured interviews. The interviews took place in the situational context of strained bilateral relations between the USA and Russia over events in Ukraine, Crimea, and Donbass, as well as economic sanctions that had been in effect for almost a year at the time of the interviews.

Findings
Respondents expressed substantial interest in vacationing in the USA despite their strong disagreement with USA international policies. While domestic tourism offerings were evaluated as inferior to those of other international destinations, patriotic sentiments with respect to domestic tourism were registered.

Practical implications
Brand USA is still very attractive to the potential tourists, especially such components as major cities, entertainment, oceans, beaches and resorts, as well as the vastness of its national parks. The absence of information with respect to travel to USA was noticed by all respondents.

Originality/value
The study is conducted in a situation of ongoing conflict between the two countries and explores how the feelings of animosity toward the USA influence the desire of Russian tourists to vacation in the USA.
Modern pioneers in marketing: autobiographical sketches by leading scholars
Authors: Tadajewski Mark , Jones D.G. Brian 
Published: March 2017

Purpose
This paper aims to introduce a special issue of the Journal of Historical Research in Marketing which includes autobiographical sketches by leading scholars in the history of marketing and consumer research.

Design/methodology/approach
A brief review of the (auto)biographical tradition in marketing scholarship leads to a commentary on the four accounts in this issue.

Findings
Highlights of the four portraits are presented and insights into their authors’ lives and careers are offered.

Originality/value
The authors hope this introductory article whets readers’ appetites to learn more about the four contributors whose careers and personal lives are explicated for their consumption.