bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of 17α-methyltestosterone and letrozole on growth and gonadal development in largemouth bass (Micropterus salmodies)
Authors: Zhang Dongyun , Li Shengjie , Tian Taihang , Du Jinxing , Lei Caixia , Zhu Tao , Han Linqiang , Song Hongmei 
Published: September 2024

In order to optimize the parameters for reversing masculinization and establish the techniques for sex induction of pseudo-males and creation of all-female fry in largemouth bass (Micropterus salmodies, LMB), 15-day-old LMB (1.00 ± 0.10 cm in length, 0.10 ± 0.01 g in weight) were fed a diet supplemented with either 17α-methyltestosterone (MT) or letrozole (LE) and their combination. The experimental groups were M20 (20 mg/kg MT), L20 (20 mg/kg LE) and M10L10 (10 mg/kg MT and 10 mg/kg LE). The control group, named C, was not feed MT or LE. After 60 days, exogenous hormone in the diets was stopped and the effects of MT and LE on growth, male ratio, and gonadal development in LMB were evaluated. At 12-month-old, blood and gonadal tissue samples were collected to measure sex steroid hormones levels, analyze expression levels of dmrt1 and cyp19a1a genes, as well as examine the gonads morphology. The results showed no significant differences in growth between the experimental groups and the control group after a 60-day feeding period with the formulated diet (p &gt; 0.05). The sex reversal ratio of M20, L20, M10L10 were 95.00%, 80.00%, 76.47%, respectively. The gonadal tissue sections showed that the gonadal structure of masculinized fish morphologic resembled that of control male fish. At 12-month-old, the sex reversal ratio in M20, L20, M10L10 and C groups were 100%, 86.67%, 73.33% and 50.00%, respectively. The testicular of pseudo-male fish in the M20 group exhibited well-developed morphology similarities to that of the control group males. However, the testes of pseudo-male fish in the L20 and M10L10 groups were smaller size Estradiol (E2) levels in the experimental groups was significantly lower than those in the control group females (p &lt; 0.05), while testosterone (T) levels were significantly higher than that of the control group (p &lt; 0.05). Compared to the female fish in the control group, pseudo-male fish from all experimental groups showed significantly upregulated expression of dmrt1 (p &lt; 0.05), and significantly downregulated expression of cyp19a1a (p &lt; 0.05). Pseudo-males selected from group M20 exhibited a significantly higher proportion of female offspring (92.00%) compared to the control group (46.50%). In summary, 20 mg/kg MT was the optimal inducing concentration.
Circular RNA expression in turkey skeletal muscle satellite cells is significantly altered by thermal challenge
Authors: Powell Ashley A. , Velleman Sandra G. , Strasburg Gale M. , Abrahante Lloréns Juan E. , Reed Kent M. 
Published: September 2024

IntroductionUnderstanding the genetic mechanisms behind muscle growth and development is crucial for improving the efficiency of animal protein production. Recent poultry studies have identified genes related to muscle development and explored how environmental stressors, such as temperature extremes, affect protein production and meat quality. Non-coding RNAs, including circular RNAs (circRNAs), play crucial roles in modulating gene expression and regulating the translation of mRNAs into proteins. This study examined circRNA expression in turkey skeletal muscle stem cells under thermal stress. The objectives were to identify and quantify circRNAs, assess circRNA abundance following RNAse R depletion, identify differentially expressed circRNAs (DECs), and predict potential microRNA (miRNA) targets for DECs and their associated genes.Materials and methodsCultured cells from two genetic lines (Nicholas commercial turkey and The Ohio State Random Bred Control 2) under three thermal treatments: cold (33°C), control (38°C), and hot (43°C) were compared at both the proliferation and differentiation stages. CircRNA prediction and differential expression and splicing analyses were conducted using the CIRIquant pipeline for both the untreated and RNase R depletion treated libraries. Predicted interactions between DECs and miRNAs, as well as the potential impact of circRNA secondary structure on these interactions, were investigated.ResultsA total of 11,125 circRNAs were predicted within the treatment groups, between both untreated and RNase R treated libraries. Differential expression analyses indicated that circRNA expression was significantly altered by thermal treatments and the genetic background of the stem cells. A total of 140 DECs were identified across the treatment comparisons. In general, more DECs within temperature treatment comparisons were identified in the proliferation stage and more DECs within genetic line comparisons were identified in the differentiation stage.DiscussionThis study highlights the significant impact of environmental stressors on non-coding RNAs and their role in gene regulation. Elucidating the role of non-coding RNAs in gene regulation can help further our understanding of muscle development and poultry production, underscoring the broader implications of this research for enhancing animal protein production efficiency.
Cognitive and motor improvement by tummy time practice in preemies from low-income settings: a randomized clinical trial
Authors: Sampaio Sabrinne Suelen Santos , Amorim Rodrigues Nathalia Allana , Souza Thalyson Luiz Gomes , Moura Julia Raffin , Azevedo Ingrid Guerra , Lima-Alvarez Carolina Daniel , Pereira Silvana Alves 
Published: September 2024

IntroductionEarly intervention and parental education for preemies are limited in some low-income countries. Thus, this study aimed to assess whether daily tummy time (TT) associated with usual care (UC) enhances motor and cognitive development in preemies from low-income countries. The main and secondary aim was to assess prone head elevation (PHE) and motor and cognitive functions, respectively.MethodsThirty-one preemies with a mean gestational age at birth of 33.3 ± 1.6 weeks and weighing &lt;2,500 g were included and 18 completed all assessments. Parents from the TT group were asked to perform TT with their preemies for at least 20 min during daily activities and playtime. Motor and cognitive functions were assessed by a blinded examiner using the motor and cognitive composite scores of Bayley-III after two and four months of corrected age. PHE was given by the angle from the tragus of the ear to the supporting surface of the infant; measurements were obtained using the Kinovea® software at baseline, after two, three, and four months of corrected age.ResultsThe Bayley-III motor composite score of the TT group was higher than the UC group after two and four months of corrected age. The PHE angle increased over time but did not differ between groups.DiscussionNevertheless, TT expanded the perspective of preemies to explore their bodies and environment, favoring the stimuli for motor and cognitive patterns. The loss of participants (31%) was one of the limitations of the study, illustrating the challenge of providing continued early interventions to preemies from low-income countries. In this sense, TT practice is a home intervention that may improve motor and cognitive function of preemies immediately after hospital discharge.
Mediation effect of knowledge management on the impact of IT capability on firm performance: exploring the moderating role of organization culture management
Authors: Wu Weiwei , Li Xue , Surangkana Bowornsintuchon 
Published: September 2024

IntroductionWith the development of the digital economy, a multitude of firms have embarked on the path of digital transformation through information technology (IT). Scholars have called for attention to the mediating or moderating mechanisms of IT capability on firm performance. This study argues that further exploration is needed regarding the relationship between IT capability and firm performance.MethodsThis study obtained questionnaire data from 152 IT senior managers of randomly selected manufacturing firms. The data was used to empirically test the proposed hypotheses using hierarchical regression analysis.ResultsThe results showed that IT capability has a positive effect on firm performance directly and indirectly via knowledge stock and knowledge process. Moreover, organizational culture management moderates the relationship between knowledge stock/knowledge process, and firm performance.DiscussionThis study proposes the “resources-knowledge-performance” mechanism, which sheds light on the “black box” of how IT capability affects firm performance. This enriches the research on knowledge from different perspectives and the the research on organizational culture by discussing the moderating role of organizational culture management in the relationship between knowledge stock/process and firm performance. Our research also has important managerial implications to firm.
Job demands-resources on digital gig platforms and counterproductive work behavior
Authors: Zhao Shanshan , Liu Yanfeng 
Published: September 2024

IntroductionWith the rapid expansion of digital gig platforms, counterproductive work behavior among gig workers has become increasingly prominent, adversely impacting the platform’s reputation, operational efficiency, and user experience. This study aims to explore how job demands and job resources influence counterproductive work behavior among gig workers.MethodsGrounded in the Job Demands-Resources model and Job Engagement Theory, this study develops a second-order chain mediation structural model to analyze the effects of job demands (Work pace/workload, physical demands, psychological demands, and customer-related social stressors) and job resources (Compensation, job security, learning opportunities, and opportunities for professional development) on counterproductive work behavior. Cognitive, emotional, and behavioral engagement are also examined as mediators.ResultsJob demands positively influence counterproductive work behavior, while job resources have a negative impact. Cognitive, emotional, and behavioral engagement each negatively affect counterproductive work behavior. Additionally, platform formalization moderates the negative influence of gig workers’ engagement on counterproductive work behavior.DiscussionThis research provides a comprehensive theoretical framework for digital gig platform managers to understand and predict gig workers’ counterproductive work behavior. It also offers practical implications for optimizing the work environment, enhancing job engagement, and mitigating counterproductive work behavior, thus fostering mutual development between gig workers and the platform.
Positive vaccine beliefs linked to reduced mental stress in healthcare professionals during COVID-19: a retrospective study
Authors: Lin Yu-Yin , Cho Shih-Feng , Hsieh Yi-Ling , Chuang Yun-Shiuan , Hsu Chia-En , Liu Yun-Chen , Sung Chia-Chi , Huang Ya-Hsiu , Ku Wen , Hsieh Meng-Hsuan , Huang Ya-Chin , Tu Hung-Pin , Wang Chao-Ling , Ho Chi-Kung 
Published: September 2024

Background and aimThe COVID-19 pandemic has led to a significant adverse effect on the mental health of healthcare professionals. This study aims to assess the effects of the prolonged pandemic on burnout and mood disorders and to evaluate the influence of positive vaccination beliefs on these factors at a medical center during the extended COVID-19 pandemic.MethodsThis retrospective study analyzed the results of an online questionnaire survey including burnout status and mood disorders from 2020 to 2022. The factors related to mood moderate/severe disorders and the impact of the positive vaccine belief were also explored.ResultsThe initial analysis revealed that healthcare professionals continued to experience significant levels of personal and work-related burnout, along with mood disorders. However, the scores and the percentage of moderate to severe burnout gradually decreased. Notably, the percentage of individuals with moderate to severe mood disorders also gradually declined (2020: 13.4%, 2021: 12.3%, 2022: 11.1%). The number of participants who need professional interventions decreased from 56.2% in 2020 to 45.9% in 2021, and 46% in 2022. Multivariate analysis revealed a positive vaccine belief was associated with a lower risk of moderate/severe mood disorders, with odd ratios (OR) and 95% confidence intervals (95% CI) of 0.38 (0.28 – 0.52) and 0.41 (0.30 – 0.52) in the 2021 and 2022 cohorts, respectively. Further investigation revealed that age over 50 was linked to a positive vaccine belief in 2021 and 2022. Within the 2022 cohort, working as nurses was identified as the independent factor associated with a less positive belief, with the OR and 95% CI of 0.49 (0.27 – 0.90).ConclusionThe findings of the present study suggest burnout and mood disorders are still significant during the pandemic. A positive vaccine belief may mitigate pandemic-related mental distress. Further interventions to enhance the belief combined with other supporting measures are important in a long fight against the pandemic.
Enhanced classification and severity prediction of major depressive disorder using acoustic features and machine learning
Authors: Liang Lijuan , Wang Yang , Ma Hui , Zhang Ran , Liu Rongxun , Zhu Rongxin , Zheng Zhiguo , Zhang Xizhe , Wang Fei 
Published: September 2024

BackgroundPrevious studies have classified major depression and healthy control groups based on vocal acoustic features, but the classification accuracy needs to be improved. Therefore, this study utilized deep learning methods to construct classification and prediction models for major depression and healthy control groups.Methods120 participants aged 16–25 participated in this study, included 64 MDD group and 56 HC group. We used the Covarep open-source algorithm to extract a total of 1200 high-level statistical functions for each sample. In addition, we used Python for correlation analysis, and neural network to establish the model to distinguish whether participants experienced depression, predict the total depression score, and evaluate the effectiveness of the classification and prediction model.ResultsThe classification modelling of the major depression and the healthy control groups by relevant and significant vocal acoustic features was 0.90, and the Receiver Operating Characteristic (ROC) curves analysis results showed that the classification accuracy was 84.16%, the sensitivity was 95.38%, and the specificity was 70.9%. The depression prediction model of speech characteristics showed that the predicted score was closely related to the total score of 17 items of the Hamilton Depression Scale(HAMD-17) (r=0.687, P&lt;0.01); and the Mean Absolute Error(MAE) between the model’s predicted score and total HAMD-17 score was 4.51.LimitationThis study’s results may have been influenced by anxiety comorbidities.ConclusionThe vocal acoustic features can not only effectively classify the major depression and the healthy control groups, but also accurately predict the severity of depressive symptoms.
Late-life suicide: machine learning predictors from a large European longitudinal cohort
Authors: Meda Nicola , Zammarrelli Josephine , Sambataro Fabio , De Leo Diego 
Published: September 2024

BackgroundPeople in late adulthood die by suicide at the highest rate worldwide. However, there are still no tools to help predict the risk of death from suicide in old age. Here, we leveraged the Survey of Health, Ageing, and Retirement in Europe (SHARE) prospective dataset to train and test a machine learning model to identify predictors for suicide in late life.MethodsOf more than 16,000 deaths recorded, 74 were suicides. We matched 73 individuals who died by suicide with people who died by accident, according to sex (28.8% female in the total sample), age at death (67 ± 16.4 years), suicidal ideation (measured with the EURO-D scale), and the number of chronic illnesses. A random forest algorithm was trained on demographic data, physical health, depression, and cognitive functioning to extract essential variables for predicting death from suicide and then tested on the test set.ResultsThe random forest algorithm had an accuracy of 79% (95% CI 0.60-0.92, p = 0.002), a sensitivity of.80, and a specificity of.78. Among the variables contributing to the model performance, the three most important factors were how long the participant was ill before death, the frequency of contact with the next of kin and the number of offspring still alive.ConclusionsProspective clinical and social information can predict death from suicide with good accuracy in late adulthood. Most of the variables that surfaced as risk factors can be attributed to the construct of social connectedness, which has been shown to play a decisive role in suicide in late life.
Predictors of the length of stay in psychiatric inpatient units: a retrospective study for the Paris Psychiatry Hospital Group
Authors: Barruel David , Perozziello Anne , Lefèvre Hassina , Msellati Annie , Launay Corine , Dauriac-Le Masson Valérie 
Published: September 2024

ObjectiveShortening the length of hospital stay (LOS) has become a major challenge for psychiatric hospitals in reducing unnecessary costs and improving the patient healthcare experience. We investigated the key factors associated with a long psychiatric hospitalization.MethodThis was a retrospective study of 8,870 full-time psychiatric hospital stays (6,216 patients) in the Paris Psychiatry Hospital Group, with a discharge in 2022. We used machine learning tools and univariate and multivariate methods to explore the impact of demographic, pathway-related, and clinical variables on the LOS.ResultsLOS &gt;30 days was associated with age &gt;55 years {odds ratio [OR] =2 [95% confidence interval 1.7–2.3]}, admission from outside the sectorization zone [OR=1.2 (1.1–1.3)], admission via a psychiatric emergency unit [OR, 1.2 (1.1–1.4)], and some clinical severity markers, such as psychotic disorder diagnosis [OR, 1.5 (1.3–1.7)], mandatory care [request of a third party, OR, 2.5 (2.1–2.9); case of imminent danger, OR, 2.3 (1.9–2.7)], the presence of seclusion and mechanical restraint measures (highlighting the positive effect of restraint duration), the somatic comorbidity for female sex [OR, 1.4 (1.2–1.7)], and treatment resistance [OR, 1.4 (1.2–1.6)]. Conversely, LOS ≤30 days was associated with being in a relationship [OR, 0.6 (0.5–0.8)], admission during a travel-related psychiatric episode [OR, 0.5 (0.3–0.6)], and personality and behavior disorders [OR, 0.7 (0.6–0.9)]. We found no significant association for features such as sex and a lack of treatment compliance.ConclusionTo our knowledge, this is the first recent study to investigate and highlight the impact of factors related to various illness severity markers, medication adherence, and patient journeys on the length of psychiatric hospital stay. A better understanding of long-stay risk factors might be helpful for optimizing the allocation of medical resources and anticipating tailored therapeutic programs.
Quantitative relationship between grip strength and quality of life in the older adult based on a restricted cubic spline model
Authors: Meng Fanhao , Zhang Yunqing , Liu Chaoxing , Zhou Cailiang 
Published: September 2024

BackgroundGrip strength have been showed diverse associations with quality of life for the older adult population in the literature, still there is lack of evidence of the threshold value of grip strength for maintaining good quality of life in older adults. The purpose of this study was to study the dose-effect relationship between grip strength and quality of life in the older adult, and to explore the factors affecting quality of life in the older adult, so as to provide effective theoretical basis for realizing healthy aging.MethodsA total of 105 older adult people over 60 years old were selected from 3 communities in Beijing. Grip strength was measured by hand dynamometer apparatus and quality of life was assessed by 36-item Short-Form (SF-36). On the basis of controlling confounding factors, the dose-effect relationship between grip strength and quality of life was analyzed with the restricted cubic spline model.ResultsThe results showed that there was a dose-effect relationship between grip strength and physical component summary (PCS) (p &lt; 0.01). However, grip strength was not significantly associated with mental component summary (MCS) (p &gt; 0.05). The threshold value of grip strength for male and female is 34.75 and 23.2 kg, for normal weight group and overweight and obesity group is 24.82 and 29.00 kg, for 60–69, 70–79, 80+ years group is 24.88, 23.37, and 22.97 kg, respectively. When the grip strength value is lower than the threshold value, the increase of grip strength was related to significant improvement of quality of life of the older adult, and when the grip strength value is higher than the threshold value, the quality of life can be maintained in good condition.ConclusionA dose-effect relationship was found between grip strength and physical health in quality of life. Results of our study indicated that the grip strength of the older adults needed to be greater than certain threshold values to maintain good quality of life.
The nexus between poverty and maternal healthcare utilization with a focus on antenatal care visits and choice of place of birth in Somaliland
Authors: Abdi Khalid Ahmed , M. K. Jayamohan , Adem Mohammed 
Published: September 2024

IntroductionPoverty poses a significant barrier to accessing healthcare globally, particularly in relation to antenatal care (ANC) visits and the use of childbirth facilities, both of which are crucial for women’s health and fetal well-being. In Somaliland, only 47% of pregnant women attend healthcare facilities for ANC, with a mere 33% receiving care from skilled birth attendants. Despite this, no previous studies have examined the relationship between poverty and maternal healthcare utilization in Somaliland. This study aims to investigate the effect of poverty on maternal healthcare utilization with focus on ANC visits and the choice of place of birth in Somaliland.MethodUtilizing data from the 2020 Somaliland Demographic Health Survey, a cross-sectional study design was employed, analyzing a nationally representative sample. The sample size used in this study was 3,183 women of reproductive age. Descriptive analysis, negative binomial regression, and multinomial logistic regression were conducted using Stata version 18.0. Diagnostic tests, including Chi-square − 2log likelihood statistic, Akaike Information Criterion, and Bayesian Information Criterion, were employed to evaluate model fit.ResultsPoverty, as indicated by wealth quintile, was associated with reduced ANC visits (adjusted incidence rate ratio (aIRR) = 0.884, 95% CI: 0.791–0.987) among women in poorer households compared to those in richer households. Women in Togdheer, Sool, and Sanaag regions had lower ANC visit rates compared to Maroodi Jeex region (aIRR = 0.803, 95% CI: 0.687–0.939; aIRR = 0.710, 95% CI: 0.601–0.839; aIRR = 0.654, 95% CI: 0.558–0.768, respectively). Women from poorer households had lower probabilities of opting for public health facilities (adjusted relative risk ratio (aRRR) = 0.457, 95% CI: 0.352–0.593) and private health facilities (aRRR = 0.195, 95% CI: 0.111–0.341) over home births compared to women in richer households. Women in Togdheer, Sool, and Sanaag regions had lower probabilities of choosing public (aRRR range: 0.331–0.175) and private (aRRR range: 0.350–0.084) health facilities for delivery over home births compared to women in Maroodi Jeex region.ConclusionPoverty significantly impedes maternal healthcare utilization, contributing to lower ANC attendance and preference for home births over public or private health facility births. Addressing these disparities requires initiatives to eliminate financial barriers, such as user fees, and enhance equitable access through community-based health insurance and improved healthcare infrastructure.