bluuebunny/crossref_metadata_2025_split huggingface dataset:
Angular Gyrus Involvement at Encoding and Retrieval Is Associated with Durable But Less Specific Memories
Authors: van der Linden Marieke , Berkers Ruud M.W.J. , Morris Richard G.M. , Fernández Guillén 
Published: September 2017

After consolidation, information belonging to a mental schema is better remembered, but such memory can be less specific when it comes to details. A neuronal mechanism consistent with this behavioral pattern could result from a dynamic interaction that entails mediation by a specific cortical network with associated hippocampal disengagement. We now report that, in male and female adult human subjects, encoding and later consolidation of a series of objects embedded in a semantic schema was associated with a buildup of activity in the angular gyrus (AG) that predicted memory 24 h later. In parallel, the posterior hippocampus became less involved as schema objects were encoded successively. Hippocampal disengagement was related to an increase in falsely remembering objects that were not presented at encoding. During both encoding and retrieval, the AG and lateral occipital complex (LOC) became functionally connected and this interaction was beneficial for successful retrieval. Therefore, a network including the AG and LOC enhances the overnight retention of schema-related memories and their simultaneous detachment from the hippocampus reduces the specificity of the memory.SIGNIFICANCE STATEMENTThis study provides the first empirical evidence on how the hippocampus and the neocortex interact dynamically when acquiring and then effectively retaining durable knowledge that is associated to preexisting knowledge, but they do so at the cost of memory specificity. This interaction is a fundamental mnemonic operation that has thus far been largely overlooked in memory research.
Whole-exome sequencing gives additional benefits compared to candidate gene sequencing in the molecular diagnosis of children with growth hormone or IGF-1 insensitivity
Authors: Shapiro Lucy , Chatterjee Sumana , Ramadan Dina G , Davies Kate M , Savage Martin O , Metherell Louise A , Storr Helen L 
Published: September 2017

Background
GH insensitivity (GHI) is characterised by short stature, IGF-1 deficiency and normal/elevated serum GH. IGF-1 insensitivity results in pre- and post-natal growth failure with normal/high IGF-1 levels. The prevalence of genetic defects is unknown.

Objective
To identify the underlying genetic diagnoses in a paediatric cohort with GH or IGF-1 insensitivity using candidate gene (CGS) and whole-exome sequencing (WES) and assess factors associated with the discovery of a genetic defect.

Methods
We undertook a prospective study of 132 patients with short stature and suspected GH or IGF-1 insensitivity referred to our centre for genetic analysis. 107 (96 GHI, 88 probands; 11 IGF-1 insensitivity, 9 probands) underwent CGS. WES was performed in those with no defined genetic aetiology following CGS.

Results
A genetic diagnosis was discovered 38/107 (36%) patients (32% probands) by CGS. WES revealed 11 patients with genetic variants in genes known to cause short stature. A further 2 patients had hypomethylation in the H19/IGF2 region or mUPD7 consistent with Silver–Russell Syndrome (total with genetic diagnosis 51/107, 48% or 41/97, 42% probands). WES also identified homozygous putative variants in FANCA and PHKB in 2 patients. Low height SDS and consanguinity were highly predictive for identifying a genetic defect.

Conclusions
Comprehensive genetic testing confirms the genetic heterogeneity of GH/IGF-1 insensitivity and successfully identified the genetic aetiology in a significant proportion of cases. WES is rapid and may isolate genetic variants that have been missed by traditional clinically driven genetic testing. This emphasises the benefits of specialist diagnostic centres.
FLUOXETINE MODULATES SEX STEROID LEVELS IN VITRO
Authors: Lupu Diana , Sjödin Marcus O. D. , Varshney Mukesh , Lindberg Johan , Loghin Felicia , Rüegg Joëlle 
Published: September 2017

Background and aims. Selective serotonin reuptake inhibitors (SSRIs) are antidepressants increasingly prescribed against depression during and after pregnancy. However, these compounds cross the placenta and are found in breast milk, thus reaching, and possibly affecting, the fetus and infant during critical developmental stages. Fluoxetine (FLX), a widely used SSRI, can interfere with estrogen signaling, which is important for the development of female sex organs and certain brain areas, among others. Interference with estrogen signaling can take place on different levels, e.g., by affecting receptor activity or hormone levels. FLX has previously been shown to induce estrogen receptor-dependent transcription in vitro at high concentrations. In this study we set out to assess effects of FLX on estradiol levels in vitro.Methods. FLX was tested using the OECD recommended H295R model, a human adrenocortical carcinoma cell line that is able to produce all steroid hormones found in the gonads and adrenal glands, including estradiol and testosterone. H295R cells were incubated with different doses of FLX for 48h. Subsequently, concentrations of these two steroids were measured in cell culture medium after FLX exposure, using liquid chromatography coupled with tandem mass spectrometry. Aromatase mRNA expression was assessed using qPCR.Results. Fluoxetine significantly increased estradiol secretion in H295R cells after a 48h exposure at low, submicromolar concentrations, but showed no effects on testosterone levels or aromatase mRNA expression.Conclusion. Fluoxetine has the potential to interfere with estrogenic signaling by increasing estradiol secretion at low concentrations, which are relevant for fetal and adult human exposure.
Endocan Serum Levels in Patients with Non-Alcoholic Fatty Liver Disease with or without Type 2 Diabetes Mellitus: A Pilot Study
Authors: Dallio Marcello , Masarone Mario , Caprio Giuseppe Gerardo , Di Sarno Rosa , Tuccillo Concetta , Sasso Ferdinando Carlo , Persico Marcello , Loguercio Carmela , Federico Alessandro 
Published: September 2017

Background & Aims: Non-alcoholic fatty liver disease (NAFLD) is emerging as an independent cardiovascular risk factor. Recently, Endocan has been studied as an early marker of endothelial dysfunction. Our aim was to evaluate Endocan serum levels in patients with NAFLD with or without type 2 diabetes mellitus.Method: We enrolled 56 patients: 19 with NAFLD and 37 with type 2 diabetes mellitus with or without NAFLD, and compared them to 25 healthy controls. Endocan serum level was measured by using the ELISA EndoMark assay.Results: Endocan level was significantly higher in NAFLD subjects, compared to controls (1.23±1.51 vs 0.68±0.4 ng/mL; p=0.016). It was higher in patients with non-alcoholic fatty liver and non-alcoholic steatohepatitis (NASH) (1.12±1.11, 1.49±2.16 and 0.68±0.4 ng/ml vs controls, respectively), independently from presence of type 2 diabetes mellitus. The increase was more marked in patients with NASH and in those with NAFL versus controls (p=0.001 and p=0.004, respectively), but not statistically different between the two groups (p=0.448). Finally, we found a statistically relevant increase of this marker in diabetic NAFLD patients compared to those non diabetic (1.56±0.81 vs 0.72±0.58 ng/ml; p=0.01).Conclusion: We demonstrated an increased Endocan serum level in NAFLD patients, higher in those with type 2 diabetes mellitus and/or NASH because of an endothelial dysfunction in these pathologies.Abbreviations: BMI: Body Mass Index; CCBS: calcium channel blockers; ED: endothelial dysfunction; ELISA: enzyme-linked immunosorbent assay; ESM-1: Endothelial cell-specific molecule-1; HOMA-IR: Homeostasis Model Assessment Insulin Resistance; ICAM: Intercellular Adhesion Molecule; LFA-1: Lymphocyte Function-Associated Antigen 1; NAFL: non-alcoholic fatty liver; NAFLD: Non-alcoholic fatty liver disease; NAS: non-alcoholic fatty liver disease activity score; NASH: non-alcoholic steatohepatitis; ROS: reactive oxigen species; T2DM: type 2 diabetes mellitus; TNF-alpha: tumor necrosis factor-alpha.
Evaluation of 2D- Shear Wave Elastography for Characterisation of Focal Liver Lesions
Authors: Gerber Ludmila , Fitting Daniel , Srikantharajah Kajana , Weiler Nina , Kyriakidou Georgia , Bojunga Joerg , Schulze Falko , Bon Dimitra , Zeuzem Stefan , Rust Mireen Friedrich 
Published: September 2017

Background & Aim: This is a prospective study for evaluation of 2D-shear wave elastography (2D-SWE) for characterisation and differentiation of benign und malignant focal liver lesions (FLLs).Methods: The patients referred to our ultrasound unit were prospectively included. B-mode ultrasound and 2D-SWE (Aixplorer® France) were performed for one FLL in each patient. Liver histology and/or contrast-enhanced imaging were used as a reference method.Results: 140 patients with FLL were included. SWE acquisitions failed in 24% of them. Therefore, 106 patients with FLL could be analysed, 42/106 with benign and 64/106 with malignant FLLs. The median stiffness for benign FLLs was 16.4 (2.1-71.9) kPa: 16.55 kPa for 18 focal nodular hyperplasia (FNH), 16.35 kPa for 18 hemangioma, 9.8 kPa for 3 focal fatty sparings (FFS), 8.9 kPa for 1 adenoma, 20 kPa for one regenerative node and 29 kPa for one cholangiofibroma, and for the malignant FLLs 36 (4.1-142.9) kPa: 44.8 kPa for 16 hepatocellular carcinoma (HCC), 70.7 kPa for 7 cholangiocarcinoma (CCC) and 29.5 kPa for the 41 metastasis (p<0.001). Malignant FLLs were significantly stiffer than benign FLLs (p<0.0001). Cholangiocarcinomas were the stiffest malignant FFLs with significantly higher values as compared to HCCs and metastases (p=0.033 and p=0.0079, respectively). No significant difference in stiffness could be observed between the different benign FLL entities. No significant difference was observed whether 2D-SWE included the whole FLL, the periphery or only the hardest area of the FLL.Conclusions: 2D-SWE provides further characterising information for interpretation of FLLs and may be useful at least in differentiation of CCCs and HCCs.Abbreviations: ALT: alanine aminotransaminase; AST: aspartate aminotransaminase; AUROC: area under the ROC; ARFI: acoustic radiation force impulse; CCC: cholangiocarcinoma; CECT: contrast-enhanced CT; CEMRI: contrast-enhanced MRI; CEUS: contrast-enhanced ultrasound; FFS: focal fatty sparing; FLL: focal liver lesion; FNH: focal nodular hyperplasia; GGT: gamma-glutamyl-transferase; HCC: hepatocellular carcinoma; NASH: nonalcoholic steatohepatitis; pSWE: point shear wave elastography; ROC: receiver-operating-characteristic; ROI: region of interest; 2D-SWE: 2D-shear wave elastography; TE: transient elastography.
Genetic Risk Factors for Autoimmune Thyroid Disease might Affect the Susceptibility to and Modulate the Progression of Primary Biliary Cholangitis
Authors: Kuś Aleksander , Grabowska Magdalena Arłukowicz , Szymański Konrad , Wunsch Ewa , Milkiewicz Małgorzata , Płoski Rafał , Shums Zakera , Norman Gary L. , Milkiewicz Piotr , Bednarczuk Tomasz , Krawczyk Marcin 
Published: September 2017

Background & Aims: Patients with primary biliary cholangitis (PBC) frequently suffer from extrahepatic autoimmune conditions, of which autoimmune thyroid disease (AITD) is one of the most common. Previous studies identified several genetic variants increasing the odds of developing AITD. Here we investigate whether AITD-associated polymorphisms might also play a role in the development and clinical course of PBC and PBC associated with AITD (PBC-AITD).Methods: To this end, we prospectively recruited 230 patients with PBC and 421 healthy controls. Among recruited patients, 64 (30.9%) had PBC-AITD as diagnosed by elevated serum TPO-antibodies. In all subjects we genotyped 10 variants previously associated with AITD.Results: We detected significant associations between the PTPN22 polymorphism and risk of developing PBC (rs2476601, OR=1.43, P=0.035) as well as PBC-AITD (OR=1.74, P=0.028). The IL2RA polymorphism was associated with liver cirrhosis (rs41295061, OR=1.76, P=0.033) whereas the MMEL1 polymorphism increased the risk of requiring liver transplantation (rs2843403, OR=1.70, P=0.023). Although no significant differences in clinical or biochemical characteristics between patients with PBC and PBC-AITD were seen (all P>0.05), liver function tests and metabolic traits in PBC patients were significantly (all P<0.05) affected by the CTLA4 (rs3087243), MMEL1 (rs2843403), PTPN22 (rs2476601) and RNASET2 (rs9355610) variants.Conclusion: Our study demonstrates the existence of a genetic overlap between PBC and AITD. Apparently, genetic variants known to increase the AITD risk might affect the clinical course of PBC. On the other hand, AITD per se does not seem to significantly influence the natural history of PBC.Abbreviations: AITD: autoimmune thyroid disease; ALP: alkaline phosphatase; ALT: alanine transaminase; AMA: anti-mitochondrial autoantibodies; AST: aspartate aminotransferase; DM1: diabetes mellitus type 1; ELISA: enzyme-linked immunosorbent assay method; GGT: gamma-glutamyl transpeptidase; GD: Graves’ disease; GWAS: genome-wide association studies; HT: Hashimoto thyroiditis; HWE: Hardy-Weinberg equilibrium; Lyp: lymphoid-specific protein tyrosine phosphatase non-receptor type 22; OR: odds ratio; PBC: primary biliary cholangitis; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; TPOAb: thyroid peroxidase antibody; UDCA: ursodeoxycholic acid.
Perbandingan Keekonomian Transmisi HVDC dan Pengangkutan Batubara Dalam Pengembangan Kelistrikan Kalimantan - Sulawesi
Authors: Liun Edwaren 
Published: September 2017

Sehubungan dengan karakteristik beban pada banyak wilayah di Indonesia, sistem kelistrikan sering tidak efisien. Di daerah yang jauh dari jaringan interkoneksi dan pulau terpencil misalnya, pemilihan jenis pembangkit berbahanbakar murah seperti batubara tidak tidak dapat diterapkan, sedangkanpembangkit berbahanbakar minyak biayanya sangat tinggi dan memberatkan beban subsidi. Akibatnya daerah tersebut tetap menggunakan pembangkit berbahanbakar minyak dengan membatasi waktu nyala pembangkit dan konsumen tidak mendapat layanan memuaskan. Makalah ini bertujuan untuk menganalisis sejauh mana kemanfaatan sistem transmisi HVDC dari Kalimantan ke Sulawesi sehingga konsumen mendapat layanan lebih baik dan dapat berproduksi untuk meningkatkan kesejahteraan. Metode yang digunakan dalam analisis ini adalah penelusuran pustaka dan melakukan perhitungan berdasarkan formula yang ada. Hasil yang diperoleh bahwa segi biaya langsung, sistem HVDC yang meliputi biaya saluran transmisi, konverter dan rugi-rugi daya berkisar antara 0,46 – 0,60 USD¢/kWh untuk kapasitas daya berkisar antara 500 – 2000 MWe. Sedangkan biaya angkut batubara berkisar antara 0,544 – 0,645 USD ¢/kWh untuk tongkang berukuran panjang antara 270 – 330 feet. Dengan demikian, untuk wilayah Indonesia dengan banyak pulau dan daerah terpencil memungkinkan secara lebih ekonomis untuk menyalurkan listrik dengan transmisi HVDC dari pulau atau daerah yang sistem listriknya telah berkembang, sehingga wilayah terpencil juga mendapat layanan listrik yang prima untuk meningkatkan kesejahteraan dan pendapatan penduduknya.
Boric Acid Radiolysis in Primary Coolant Water of PWR at Temperature of 250oC
Authors: Sunaryo Geni Rina 
Published: September 2017

BORIC ACID RADIOLYSIS IN PRIMARY COOLANT WATER OF PWR AT TEMPERATURE OF 250oC. The existence of oxygen in the primary coolant system of PWR could lead to corrosion, hence it is very important to suppress the oxygen concentration in the system. Therefore, study of the effect of boric acid addition into the primary coolant water system of PWR to suppress oxygen concentration resulted from gamma-ray radiation is essential to be performed. The aim of this research is to understand reaction mechanism at temperature of 2500C and the effect of boric acid adding toward oxygen concentration in the PWR primary coolant water. Methodology used is simulation using Facsimile software. Input for the software namely radiolysis reaction mechanism for pure water, G value from radiolysis product, dose rate of 1 and 104 Gy/s, aeration and deaeration system, and specific reaction of boric acid with hydroxyl radical and hydrated electron at temperature 250C and 3000C. The output are in the form of irradiation time vs oxygen concentration time series. The results show that the oxygen production increase significantly with the irradiation time and reach the saturated concentration at 107s. Based on the plot of oxygen’s concentration at 107s vs boric acid, several results are as following: oxygen concentration significantly suppressed by boric acid addition and gives the exponential decreasement, the higher dose rate gives the higher concentration of oxygen, the aeration system gives no effect on suppressing oxygen concentration at boric acid addition up to 0.1M.
Analisis Kinerja Bahan Bakar Reaktor Tipe HTGR Sebagai Penghalang Produk Fisi
Authors: Dewita Erlan , Alimah Siti 
Published: September 2017

ANALISIS KINERJA BAHAN BAKAR REAKTOR TIPE HTGR SEBAGAI PENGHALANG PRODUK FISI. Reaktor tipe HTGR merupakan reaktor berpendingin gas temperatur tinggi (~ 900oC). Terdapat 2 tipe elemen bakar HTGR yaitu prismatik dan pebble bed. Kedua tipe elemen bakar tersebut tersusun dari partikel berlapis TRISO yang terdiri dari lapisan IPyC, SiC dan OPyC yang berfungsi sebagai pengungkung produk fisi dan menjaga integritas bahan bakar. Reaktor beroperasi dengan temperatur tinggi, sehingga kinerja/ kemampuan bahan bakar dalam menahan produk fisi perlu diketahui. Tujuan studi adalah untuk memperoleh pemahaman tentang karakteristik produk fisi yang dihasilkan bahan bakar, karakteristik penghalang dan kinerja bahan bakar dalam menahan produk fisi. Metode yang digunakan adalah kajian dan analisis dengan mengevaluasi kemampuan penghalang (barrier) dalam menahan produk fisi pada elemen bakar prismatik dan pebble. Hasil studi menunjukkan bahwa terdapat beberapa mekanisme potensial lepasnya produk fisi, yaitu: difusi melalui lapisan, kerusakan lapisan, korosi SiC oleh produk fisi palladium dan dekomposisi termal SiC. Bahan bakar merupakan penghalang pertama terhadap lepasnya radionuklida produk fisi sedangkan lapisan SiC merupakan penghalang utama yang menahan sebagian besar produk fisi gas dan padat pada temperatur operasi normal (< 1250°C). Produk fisi penting yang terbentuk adalah 137Cs, 107 Pd, 106Rh, 106Ru, 110mAg, 134I, 131Cs, 137Cs, 90Sr, 88Kr dan 133Xe, 132Te, 140La dan 239Pu. Di antara produk fisi tersebut, paladium (Pd) yang lepas dari kernel dan mencapai lapisan SiC dapat bereaksi dan menyebabkan korosi. Berdasarkan hal itu, untuk menjaga integritas bahan bakar harus dilakukan pembatasan kondisi operasi reaktor, seperti: temperatur, derajat bakar, energi aktivasi produk fisi dan kualitas bahan bakar. Pada bahan bakar tipe prismatik, terdapat 8 penghalang, yaitu: kernel bahan bakar, lapisan SiC dan PyC, matriks grafit, kompak (pil) bahan bakar, sleeve grafit, sirkuit primer, blok grafit heksagonal dan bangunan reaktor. Sedangkan pada bahan bakar pebble terdapat 6 penghalang, yaitu kernel bahan bakar, lapisan SiC dan PyC, matriks grafit, lapisan grafit sebelah luar, sirkuit primer dan bangunan reaktor. Namun, jumlah penghalang bukan merupakan satu-satunya faktor yang menentukan adanya kemungkinan lepasnya produk fisi ke lingkungan.
Performa Ayam Broiler yang Mengkonsumsi Kulit Nanas yang Difermentasi dengan Yogurt dalam Ransum Mengandung Gulma Obat
Authors: Nurhayati Nurhayati , Berliana Berliana , Nelwida Nelwida 
Published: September 2017

ABSTRAK. Penelitian bertujuan untuk mengetahui performa ayam broiler yang mengkonsumsi kulit nanas yang difermentasi (KNF) dengan yogurt dalam ransum mengandung gulma obat (GO). Materi yang digunakan yaitu 200 ekor ayam broiler jantan umur 2 hari, ransum mengandung gulma obat dan kulit nanas yang difermentasi dengan yogurt. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Perlakuan yang diberikan yaitu T0 (ransum kontrol, ransum mengandung 0 % KNF dan 0 % GO), T1 (ransum mengandung 0 % KNF dan 2 % GO), T2 (ransum mengandung 7,5 % KNF dan 2 % GO), T3 (ransum mengandung 15 % KNF dan 2 % GO), dan T4 (ransum mengandung 22,5 % KNF dan 2 % GO). Peubah yang diamati adalah konsumsi ransum, pertambahan bobot badan (PBB), bobot akhir dan konversi ransum. Data dianalisis menggunakan ANOVA dan Uji kontras ortogonal. Hasil penelitian menunjukkan bahwa pemberian ransum yang mengandung KNF berpengaruh nyata (P0.05) terhadap konsumsi ransum, PBB, bobot akhir, dan konversi ransum. Konsumsi dan konversi ransum meningkat dengan peningkatan pemberian KNF sedangkan bobot badan menurun. Disimpulkan bahwa pemberian tepung kulit nanas setelah difermentasi dengan yogurt sebanyak 22,5 % di dalam ransum mengandung campuran gulma obat dapat menurunkan performa ayam broiler. (Broiler chicken performance fed fermented pineapple peel by yogurt in diet containing medicinal weed)ABSTRACT. Research aimed to determine broiler performance fed different level of yoghurt - fermented pineapple peel (FPPM) in the diet containing medicinal weed. Two hundred 2 day - old male broiler chicken, diet containing medicinal weed (MW) and yoghurt - fermented pineapple peel used in this study. The experimental design was a completely randomized design with 5 treatments and 5 replications. The treatments were T0 (control, diet containing 0% FPPM and 0% MW), T1 (diet containing 0% FPPM and 2% MW), T2 (diet containing 7.5% FPPM and 2% MW), T3 (diet containing 15% FPPM and 2% MW), and T4 (diet containing 22.5% FPPM and 2% MW). The variables were feed consumption, body weight gain, live weight and feed conversion ratio. Data were analyzed by ANOVA and Contrast orthogonal Test. The results showed that there was a significant effect (P0,05) of treatment on the feed consumption, body weight gain, live weight and feed conversion ratio. Feed consumption and feed conversion ratio increased with increasing level of FPPM in the diet, however, body weight decreased. It is concluded that feeding yoghurt - fermented pineapple peels up to 22,5 % in the broiler diet containing medicinal weed decreased broiler chicken performance.