bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glucose oxidase synergistically promotes cellulase hydrogenation distillation for the extraction of essential oil from Eleutherococcus senticosus
Authors: Zhao Xu , Chen Zeshao , Xi Gaolei , Zhao Shengchen , Cao Ke , Wang Qiyue , Zhang Yinghao , Ma Shengtao , An Nan , Yu Shuai , Jia Tao 
Published: October 2024

Abstract

Eleutherococcus senticosus essential oil (ESEO) has the function of clearing heat and detoxifying, delaying aging. The ESEO yield obtained by traditional extraction methods is low because essential oils are encased in plant cell structures and are difficult to extract. In this study, we adopted the method of glucose oxidase promoting cellulase assisted hydrogenation distillation(GCAHD), and utilized the interaction of glucose oxidase and cellulase to promote the full destruction of cellulose in plant cells, resulting in a large amount of internal essential oil flowing out and improving the yield of ESEO. By optimizing the main factors of enzymatic hydrolysis, the optimal extraction conditions for E. senticosus essential oil were determined through BBD experiments. The ratio of glucose oxidase to cellulase is 1:20, the concentration of the enzyme is 1.02%, the temperature of enzymatic hydrolysis is 40.0 ℃, and the pH value of enzymatic hydrolysis is 3.44. The optimal yield was 0.14%. The yield of essential oil extracted from E. senticosus was increased by 49.9% compared to a single cellulase assisted extraction method. The extraction process used in this work is milder, more environmentally friendly, and more efficient, which has important reference value for the industrial extraction and utilization of medicinal plants.
Postmarketing safety of eltrombopag olamine : an analysis of the FDA Adverse Event Reporting System (FAERS)
Authors: Wang Kun , Xu Peipei , Lu Sheng , Yao Bin 
Published: October 2024

Abstract

Objective This study aims to investigate the risk factors associated with adverse events related to the novel thrombopoietin receptor agonist, eltrombopag olamine, with the intention of providing guidance for safe clinical medication practices.
Methods Adverse drug event (ADE) reports from the US Food and Drug Administration's Adverse Event Reporting System (FAERS) spanning from the first quarter of 2004 to the third quarter of 2023 were collected and subjected to statistical analysis based on the System Organ Classification (SOC) and Preferred Terms (PT) as defined by the International Dictionary of Medical Terms (MedDRA). The report ratio method (ROR) and the proportional report ratio method (PRR) were used.
Results A total of 10,357 adverse drug reaction (ADR) reports, with eltrombopag olamine identified as the primary suspected drug, were retrieved from the FAERS database. These reports included 228 risk signals at the Preferred Term (PT) level and 22 signals overall. The three most frequently reported adverse events were decreased platelet count, death and increased platelet count. The three adverse events with the highest signal intensity were increased reticulin, reticular fibrosis of bone marrow and leukopenia, respectively.Among the adverse reactions not specified in the instruction manual for eltrombopag olamine are ascites associated with gastrointestinal diseases, blistering of the oral mucosa, iron deficiency, and emaciation related to metabolic and nutritional disorders, as well as Alzheimer's dementia among various nervous system diseases, and other signals. There is no conclusive evidence to dismiss a correlation with eltrombopag olamine. The primary risks associated with SMQ drugs include hematopoietic cytopenia, various forms of bleeding, embolism, and thrombosis.
Conclusion Eltrombopag olamine has been demonstrated to be safe, with the majority of prominent risk signals aligning with known adverse reactions associated with the blood and lymphatic systems, as outlined in the prescribing information. It is recommended that clinicians exercise caution regarding the potential risks of paroxysmal nocturnal hemoglobinuria, abnormal platelet counts, hemorrhage, and hearing loss when administering eltrombopag olamine in a clinical setting.
Rapid and cost-effective screening of genetic markers associated with pyrethroid resistance in Musca domestica using RNAse H2 PCR (rhPCR)
Authors: Estep Alden , Sanscrainte Neil , Pagac Alexandra , Geden Christopher , IV Edwin Burgess 
Published: October 2024

Abstract

Pyrethroid resistance, particularly knockdown resistance (kdr), is widespread in insect pest populations, but rarely has kdr been associated with field-level pest control failure. The prevailing understanding is that kdr contributes to a resistant phenotype, but this knowledge has remained largely an academic pursuit and has not translated to tools and strategies needed by agricultural producers to make rapid decisions for effective resistance management. As a first step in providing these operational tools, we developed robust assays using the high specificity of rhPCR to reduce kdr assessment time by approximately 80% and costs ~ 75% from the traditional Sanger based method used for Musca domestica. An important consideration for the use of an operational tool is the ability to get an accurate result on the first attempt, so we used Nanopore sequencing to confirm genotypes in a subset of samples and found the first pass genotyping accuracy of rhPCR method to be 75.0%, versus 41.2% with the traditional Sanger method. To demonstrate the broad applicability and comparability of screening for kdr SNPs using rhPCR, we conducted the largest assessment of kdr genotypes of M. domestica in United States dairy operations and found similar kdr patterns to other recent studies using traditional methods.
Serotype patterns of 2022 and 2023 dengue outbreaks in Chattogram, Bangladesh
Authors: Biswas Rajat Sanker Roy , Sequeira Austin , Hasan Anamul , Zamil Md Fahad , Alam Mohammad Shafiul , Bishop Jean 
Published: October 2024

Abstract

Background: Dengue viruses have been prevalent since 2000 in Bangladesh, including Chattogram. The objective of the present study was to examine the serotype patterns of dengue viruses in 2022 and 2023 in Chattogram, the southern part of Bangladesh.
Methods: Present study was conducted in Chattogram Maa O Shishu Hospital from June through December, 2022 and October through December 2023. Patients clinically suggestive of dengue fever and blood NS1 test positive, were recruited in the study. A total of 80 (2022 - 68 cases and 2023-12 cases) patients were undergone serotype testing. Serotyping of 2022 samples were done in the SlieaGen L.L.C testing lab Texas, USA and 2023 samples done in ICDDR’B Bangladesh.
Results: In 2022, 68 samples underwent qPCR, and dengue viruses were identified in 27 cases (40%). Among the 27 positive cases, DENV-1 was detected in 25 samples (36.8%), DENV-2 in 3 samples (4.5%), DENV-3 in 16 samples (23.6%), and DENV-4 in 1 sample (1.5%). The distribution of serotypes in individual samples showed that DENV-1 was found in 10 samples (14.7%), while DENV-3 and DENV-4 were each found in one sample. Combined serotypes were also observed: DENV-1 + DENV-3 in 12 samples (17.6%) and DENV-1 + DENV-2 + DENV-3 in 3 samples (4.1%). In 2023, 12 samples underwent qPCR, with dengue viruses identified in 10 cases (83.3%). One sample was insufficient, and another tested PCR-negative. Of the positive cases, 9 samples (75%) were DENV-2, and 1 sample (8.3%) was DENV-1. No samples tested positive for both serotypes, and no DENV-3 or DENV-4 cases were detected that year.
Conclusions: All four serotypes were found in the 2022 but DENV-1 and DENV-3 were found more common. Mostly DENV-2 and few DEN-1 serotypes were found in the year 2023 in Chattogram. Simultaneous double and triple serotype infection in the single patients were the interesting finding in 2022 dengue outbreak. A serotype shift from DENV-1 to DENV-2 occurred in Chattogram, Bangladesh, in 2023.
Cross-sectional study to track antibiotic consumption and its appropriateness in an intensive care setting of a tertiary care hospital using AWaRe tool of World Health Organization (WHO).
Authors: Raj Grace Jabez , Balan Aravinda Kumar , Rajaram Saranya , Pandy Madhu 
Published: October 2024

Abstract

Background:
Incorporating AWaRe classification metrics provide a structured approach to evaluating antibiotic consumption and guiding future interventions for better antibiotic stewardship in healthcare facilities. The study aimed to quantify antibiotic consumption and assess its appropriateness in an intensive care unit of a tertiary care hospital in South India using the WHO's AWaRe tool.
Methods:
This study was conducted in a South Indian ICU over 2 months, including prescriptions with at least one antibiotic for patients admitted within 2 weeks. Around 100 prescriptions were included based on ICU admission statistics. An AWaRe tool by WHO was used to analyze antibiotic consumption and appropriateness.
Results:
201 antibiotics were prescribed, with an average of 2 antimicrobials per patient. 78.6% administered parenterally, 21.4% orally. 29% cases had clinical samples sent for Culture & Sensitivity testing. Only 29% cases showed escalation or de-escalation of antimicrobials based on clinical response.
Conclusions:
The study highlighted the empirical nature of antibiotic prescriptions, limited microbiological guidance, and inadequate adjustments based on clinical responses. These findings emphasize the need for improved guidelines and practices to optimize antibiotic use in intensive care settings, ensuring appropriateness and effectiveness in patient care.
First report on the long-term viability of Acanthamoeba species in unpreserved environmental freshwater samples stored at room temperature
Authors: Masangkay Frederick R. , Rona IV Fausto C. , Poricallan Angel Jasmine G. , Padua Raphael Louis M. , Reyes Marielle Sabina S. , Ricacho Justine Kate M. , Samar Monica Jane E. , Santos Maria Carmela I. , Tangpong Jitbanjong , Kotepui Manas , Padua Mark F. F. E. , Milanez Giovanni D. , Karanis Panagiotis 
Published: October 2024

ABSTRACT

Acanthamoeba is an opportunistic, free-living amoeba ubiquitous in the environment. Despite reports of its wide distribution in the Philippines’ freshwater resources, more information on the long-term viability of the Acanthamoeba species is needed. This study aimed to define the long-term viability of Acanthamoeba species in unpreserved environmental freshwater samples after 3 years of storage at room temperature. Stored water samples from 15 study sites were filtered through a 1.2-μm pore size glass microfiber filter, cultured in non-nutrient agar (NNA) lawned with Escherichia coli, and observed for amoebic growth for 14 days using light microscopy. Isolates from positive NNA culture were subjected to polymerase chain reaction (PCR) using JDP1 and JDP2 Acanthamoeba-specific primers. The study site positivity was 33% (5/15). Acanthamoeba genotype T4 and Acanthamoeba lenticulata were isolated from Luzon; Acanthamoeba divionensis was isolated from Visayas; and Acanthamoeba sp. and genotype T20 were isolated from Mindanao. The long-term viability of Acanthamoeba species is an added risk factor for the sustained contamination of aquatic resources and other sample matrices. This heightens the risk of transmission to humans and animals. This study demonstrated that water samples fated for Acanthamoeba studies can be stored unpreserved at room temperature for several years.
Collaborative water governance model for potable urban water supply in Riau Province, Indonesia
Authors: Suyeno Suyeno , Sumartono Sumartono , Haryono Bambang Santoso , Amin Fadillah 
Published: October 2024

ABSTRACT

The literature suggests that collaborative governance is a promising framework for addressing complex public problems while some cases have also marked failure in its implementation. Furthermore, the complexities and factors of the collaborative governance context must be understood at the early stage, as they may affect its sustainability. Therefore, this study aims to understand ‘what works and what doesn't’ and the need for sustainability of urban potable water management in Dumai City, Rokan Hilir Regency, and Bengkalis Regency through cross-sectoral collaboration and develop the best model to be developed for this collaboration. This study uses a soft system methodology in the urban potable water supply system of Riau Province with a wide range of stakeholders through in-depth interviews, thematic analysis, and the creation of purposeful activity models and rich pictures to uncover and address complex issues. The study found that the implementation of collaborative water management in urban areas with the collaborative water governance approach has worked well in the short term. However, it has not enhanced organizational autonomy or fostered trust and reciprocity dimensions in the long term. Additionally, we emphasize the importance of a collaborative supervisory board that will open internal and external opportunities.
The limitation of machine learning methods for water supply and demand forecasting: A case study for Greater Melbourne, Australia
Authors: Mohammadi Maryam , Gato-Trinidad Shirley , King Kuok Kuok 
Published: October 2024

ABSTRACT
Many cities around the world have faced water scarcity due to climate change, population growth, and urbanization. Accurate water supply and demand forecasting is critical for sustainable urban water management. Machine learning (ML) models provide new possibilities for forecasting compared with traditional models in handling non-linearity. This study aims to address the efficacy of ML models, long short-term memory (LSTM), stacked LSTM, bidirectional LSTM (Bi-LSTM), and multilayer perceptron (MLP), for forecasting water supply and demand in Greater Melbourne, Australia. The ML modelling utilized daily water supply and demand, and climatic variables (rainfall and maximum temperature) recorded by Melbourne Water and the Bureau of Meteorology from 1990 to 2019. The stacked LSTM performs better than other models in forecasting with R2, RMSE, and MAPE values of 0.908, 335.74 ML, and 23.5% for water supply and 0.791, 94.88 ML, and 5.3% for water demand, respectively. The inclusion of climatic variables enhanced the accuracy of forecasting by improving R2 and reducing RMSE and MAPE. The results indicate effectiveness of ML models, particularly LSTM-based architectures, in forecasting water supply and demand. However, these models have limitations, particularly in forecasting extreme values, emphasizing the need to improve ML models for more reliable and accurate water management.
Groundwater potential mapping and its sustainable management using AHP and FR models in the Jedeb watershed, Upper Blue Nile Basin, Ethiopia
Authors: Asrade Tadie Mulie 
Published: October 2024

ABSTRACT
Groundwater potential mapping and its sustainable development are important aspects of the Jedeb watershed due to an increase in groundwater demand and the local population. This study aimed to delineate potential groundwater zones in the Jedeb watershed, using the analytical hierarchy process (AHP) and frequency ratio (FR) model. Nine thematic layers, including lithology, geomorphology, slope, drainage density, land cover and land use, lineament density, rainfall, soil, and static water level, were considered. The thematic layers obtained were integrated into a Geographic Information Systems (GIS) environment to generate the groundwater potential map. Four classes of groundwater potential have been identified: low, moderate, high, and very high. The low classes represent 12.8% and 7.02%; moderate 25.35% and 34.84%; high 38.8% and 33.11%; and very high 23.05% and 25.03%, for the AHP and FR models, respectively. The result map was also validated by using receiver operating characteristic (ROC) curve from which result accuracy was obtained is 85.6% and 88.3% for the AHP method and FR method, respectively. The study's validation verified that the FR model produced more efficacious results than the AHP model. This study, will be helpful in providing planners, and decision-makers with rapid decision-making when it comes to managing groundwater resources.
Mitochondrial Dysfunction Associated with mtDNA Mutation: Mitochondrial Genome Editing in Atherosclerosis Research
Authors: Khotina Victoria A. , Vinokurov Andrey Y. , Sinyov Vasily V. , Zhuravlev Alexander D. , Popov Daniil Y. , Sukhorukov Vasily N. , Sobenin Igor A. , Orekhov Alexander N. 
Published: October 2024

Background:
Atherosclerosis is a complex cardiovascular disease often associated
with mitochondrial dysfunction, which can lead to various cellular and metabolic
abnormalities. Within the mitochondrial genome, specific mutations have been implicated
in contributing to mitochondrial dysfunction. Atherosclerosis-associated
m.15059G>A mutation has been of particular interest due to its potential role in altering
mitochondrial function and cellular health.

Objective:
This study aims to investigate the role of the atherosclerosis-associated
m.15059G>A mutation in the development of mitochondrial dysfunction in monocyte--
like cells.

Methods:
Monocyte-like cytoplasmic hybrid cell line TC-HSMAM1, which contains
the m.15059G>A mutation in mtDNA, was used. The MitoCas9 vector was utilized to
eliminate mtDNA copies carrying the m.15059G>A mutation from TC-HSMAM1 cybrids.
Mitochondrial membrane potential, generation of reactive oxygen species, and
lipid peroxidation levels were assessed using flow cytometry. Cellular reduced glutathione
levels were assessed using the confocal microscopy. The oxygen consumption
rate was measured using polarographic oxygen respirometry.

Results:
The elimination of the m.15059G>A mutation resulted in a significant increase
in mitochondrial membrane potential and improved mitochondrial efficiency while also
causing a decrease in the generation of reactive oxygen species, lipid peroxidation, as
well as cellular bioenergetic parameters, such as proton leak and non-mitochondrial oxygen
consumption. At the same time, no changes were found in the intracellular antioxidant
system after the mitochondrial genome editing.

Conclusions:
The presence of the m.15059G>A mutation contributes to mitochondrial
dysfunction by reducing mitochondrial membrane potential, increasing the generation of
reactive oxygen species and lipid peroxidation, and altering mitochondrial bioenergetics.
Elimination of the mtDNA containing atherogenic mutation leads to an improvement in
mitochondrial function.
Isolation, Development and Validation of Chromatographic Methods for the Estimation of Linoleic Acid from Different Parts of Euphorbia Neriifolia Linn.
Authors: Chaudhary Priya , Singh Devendra , Meena Mukesh , Janmeda Pracheta 
Published: October 2024

Objectives:
This is the first report on the development and validation of thin-layer chromatography
(TLC) and high-performance thin-layer chromatography (HPTLC)-densitometric methods
for the identification of linoleic acid (LA) in petroleum ether extract (PEE) of Euphorbia neriifolia
(EN) stem (ST), latex (LX), and bark (BA).

Methodology:
Chromatographic analyses were performed on silica gel-G and silica gel 60 F254
plates and the antioxidant activities of isolated compounds were investigated by 2,2-diphenyl-1-
picrylhydrazyl (DPPH) spectrophotometric assay.

Results:
The chromatographic analyses revealed better spots and well-separated peaks of LA with
retention factor (Rf) values at 0.54 (ST), 0.40 (LX), and 0.64 (BA), respectively. The linearity of the
calibration curve ranges from 10-50 ng/spot (ST), 10-100 ng/spot (LX), and 50-200 ng/spot (BA).
The proposed method was characterized by better accuracy, better robustness, and good precision,
ranging from 0.173 to 0.372% (intra-day) and 0.185 to 0.205% (inter-day). The value of the limit of
detection and quantification equal to 1.04 and 3.16 ng/spot in ST, 0.87 and 2.64 ng/spot in LX, and
0.177 and 0.53 ng/spot in BA determined the sensitivity of the method. In the obtained chromatogram,
no peak was observed other than the LA which determined the specificity of the method. The
% RSD of < 2% after periods of 12, 24, 36, 48, and 72 h determined the stability of standard LA.

Conclusion:
Thus, the fingerprinting method is valuable in determining the adulterants and in routine
quality control of formulations and herbal drugs.
Polyene Macrolactams from Marine Micromonospora sp.
Authors: Zhao Wei , Zhou Jian , Sun Fei , Jiang Honglei , Lin Ru , Zhou Jingming , Chen Xiuming , Butt Usman-Dawood , Wang Yi-nuo , Jiang Hong , Wu Bin 
Published: October 2024

Introduction:
Three novel macrolactams, FW8-1 (1), FW8-4 (2), and FW60-20 (3), were
isolated from a culture of Micromonospora sp. The structures of these compounds were elucidated
using Mass Spectrometry (MS) and comprehensive Nuclear Magnetic Resonance (NMR) analyses.

Methods:
The relative configurations of compounds 1-3 were assigned through theoretical calculations
of their NMR spectra.

objective:
The objective is to obtain PMLs with novel structures and to characterize and evaluate them.

Results:
The isolation and determination of the relative configurations of these macrolactams have
provided fresh perspectives on the biosynthetic pathways, leading to the formation of polyene macrolactams.

Conclusion:
Further, virtual screening and bioactivity predictions have suggested compounds 1-3 to
possess potential anti-tumor, anti-inflammatory, and neuroprotective properties.