bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of fermented liquid food and zinc bacitracin on microbial metabolism in the gut and sensoric profile ofm. longissimus dorsifrom entire male and female pigs
Authors: Hansen L. L. , Mikkelsen L. L. , Agerhem H. , Laue A. , Jensen M. T. , Jensen B. B. 
Published: August 2016

AbstractThe aim of this work in pigs was to evaluate the effect of fermented liquid food (FLF) and the combination of FLF plus short-term addition of an antibiotic food additive zinc bacitracin (FLF + ZB) compared with fed non-pelleted dry food (NPDF), on microbial metabolism in the gut and the effects on flavour and odour attributes and profiles of pig meat as well as boar odour from entire male and female pigs.At an average start weight of 60 kg, 108 pigs (54 males and 54 females) were equally allocated to three treatments according to pen-replicate, litter and sex. Microbial metabolism in the gut was studied in 24 pigs, eight from each treatment. For sensory profile evaluation (flavour, odour and tenderness) of pork loins from m. longissimus dorsi, 48 pigs (24 entire males and 24 females) were selected. The sensory evaluation of different qualities of flavour and odour as well as tenderness of cooked pork loins (LD) was done by a boar taint (skatole and androstenone) trained taste panel. To evaluate the odour and the flavour, a sensory profile analysis was performed which involved the following attributes: total off-odour/off-flavour, pig, urine, manure, naphthalene, rancid, sweet and sweat in the whole sample including both meat and fat.Giving FLF to pigs significantly changed the microbiota in the gastro-intestinal tract compared with NPDF. In particular, the density of coliform bacteria was reduced in the gastro-intestinal tract of the pigs on FLF. Giving FLF demonstrated no effects on skatole concentration in caecum, colon, blood and backfat and boar odour attributes, whereas administration of FLF + ZB decreased the skatole concentrations and the typical boar odours, pig and manure odour, compared especially with the pigs on NPDF. However, meat from pigs given FLF either with or without zinc bacitracin had smaller but significantly worsened scores for three flavour attributes — pig flavour, rancid flavour and total off-flavour — compared with meat from NPDF pigs and the three flavour attributes were equally affected in both sexes. This seems to point to a significant worsening of meat flavour in pigs given FLF that is independent of sex and boar odour problems (skatole and androstenone).
Long-term changes in performance and meat quality of Danish Landrace pigs: a study on a current compared with an unimproved genotype
Authors: Oksbjerg N. , Petersen J. S. , Sørensen I. L. , Henckel P. , Vestergaard M. , Ertbjerg P. , Møller A. J. , Bejerholm C. , Støier S. 
Published: August 2016

AbstractAn experiment was conducted in 1995 in order to examine muscle growth, muscle histochemical properties, muscle metabolism and meat quality of two types of Danish Landrace pigs representing the growth potential of years 1976 and 1995, respectively. Danish Landrace pigs representing 1976 (slow-growing, SG) originated from a breeding station where the population was maintained without being selected for production traits such as daily gain, food conversion ratio and meat content. Pigs representing Danish Landrace of 1995 (fast-growing, FG) were bought from certified Danish pig breeders. The pigs were simultaneously tested for performance from 40 to 95 kg live weight. The daily gain, food conversion ratio and meat content of the carcass were improved proportionately by 0·43, 0·24 and 0·03 in FG pigs compared with SG pigs. In m. longissimus dorsi (LD), the cross-sectional area of muscle fibres was smaller in FG pigs compared with SG pigs indicating increased muscle fibre number. Data further suggest increased satellite cell proliferation in muscles of FG pigs. Serum from FG pigs stimulated the proliferation of C2C12 muscle cells to a greater extent than serum from SG pigs, although the serum level of insulin-like growth factor 1 did not differ between pig types. The effect of serum on protein turn-over of C2C12 myotubes did not depend on pig type. The glycogen concentration in the LD did not differ significantly between pig types, while the activity of citrate synthase, 3-OH-acyl-CoA-dehydrogenase, and lactate dehydrogenase were higher in the LD of SG pigs compared with FG pigs. Pork chop colour of FG pigs was proportionately 0·09 lighter (L⋆) and 0·13 less red (a⋆) than pork chops of SG pigs. The total muscle pigment concentration in the LD, m. biceps femoris and m. vastus intermedius was proportionately reduced by 0·17, 0·19 and 0·11, respectively, in FG pigs compared with SG pigs. In the LD, the concentration of myoglobin was proportionately reduced by 0·17 in FG pigs. The chemical composition of the LD differed between pig types such that the water content was higher and protein content lower in LD from FG pigs compared with SG pigs. The pH of the LD measured 45 min and 24 h post mortem, drip loss and thawing loss were similar for both pig types. Meat tenderness of conditioned pork chops of FG pigs was slightly reduced in accordance with a decreased intensity of the 31 kDa peptide band and increased cooking loss. The present study suggests that increased muscle fibre number and rate of muscle DNA deposition (satellite cell proliferation) have contributed to the increased muscle growth as a result of selection for performance in pigs under Danish conditions. However, the increase in growth performance was accompanied by deterioration in muscle colour and slightly reduced tenderness.
Quantitative analysis of methionine and cysteine requirements for wool production of sheep
Authors: Liu S. M. , Masters D. G. 
Published: August 2016

AbstractThe conventional system of estimating metabolizable protein requirement is unsuitable for defining amino acid requirements because nitrogen loss cannot be converted to amino acid loss, and because there is a lack of data on the utilization efficiencies of individual amino acids for various productive purposes. Therefore, we are proposing an alternative approach. In this review, we use methionine (Met) and cysteine (Cys) for wool production in sheep as examples, and define requirement as retention in both body and wool protein, plus the amount of obligatory oxidation that accompanies the retention. The requirements are expressed in terms of the net absorption. Mathematical models for the calculation of the requirements of both amino acids are established based on the level of absorption, endogenous contribution from body protein breakdown, oxidation rates and the amino acid composition of body and wool proteins. The flows and oxidation of Met and Cys, and Cys synthesis de novo as well are quantified using their plasma kinetics data. Wool growth rate is predicted from the amount of the amino acid available for protein retention and the partition ratio to wool growth. The estimated requirements for Met and Cys absorption for Merino sheep at maintenance are 0·45 to 0·75 g/day and 0·52 to 0·63 g/day depending on the live weight of the sheep. When wool growth rate increases to 10 g/day, the requirements increase to 0·91 to 1·24 g/day and 1·97 to 2·02 g/day respectively. The utilization efficiency for protein retention varies with the level of absorption, and is 0·02 to 0·55 for Met, and 0·09 to 0·55 for Cys. The model shows that wool growth rate is restricted by the lack of Cys supplied in conventional diets and is very sensitive to changes in oxidation of the amino acids.
Feeding behaviour, food intake and milk production responses of lactating dairy cows to diets based on grass silage of high or low dry-matter content, supplemented with quickly and slowly fermentable energy sources
Authors: Romney D. L. , Blunn V. , Sanderson R. , Leaver J. D. 
Published: August 2016

AbstractTwelve dairy cows in early lactation were offered low (L; 215 g/kg) or high (H; 449 g/kg) dry matter(DM) content silages, prepared using material from the same sward. In addition, all animals received 9 kg/day, of supplements based on barley (B), sugar-beet pulp (SB) or a 50: 50 mixture of the two (B: SB), in two equal portions at 07:30 and 14:30 h. The six treatments were offered in an incomplete Latin square design. Mean intakes of H (14·4 kg DM per day) were significantly higher than intakes observed for L (10·0 kg DM per day) (P < 0·001). Within silage type, highest intakes were observed for cows receiving the SB supplement (P < 0·01). Higher intakes of H were reflected in higher total milk yield (P < 0·05) as well as fat (P < 0·05) and protein (P < 0·01) yield. Milk protein concentration was greater for animals receiving silage H (P < 0·001), with lower values being observed for animals consuming SB (P < 0·05), within silage type. Time spent eating, duration and number of meals were similar for either silage and the higher intakes of H silage reflected greater intake rates (g DM per min) (P < 0·001) resulting in larger meal sizes (P < 0·001). All chewing indices (time spent eating silage, ruminating and total time chewing per kg DM ingested) were greater for the L silage (P < 0·001). It is concluded that the benefits in forage intake with higher DM grass silages, for high yielding dairy cows, are associated with consequential benefits in milk yield and milk protein content. The most likely explanation for the greater intakes is a faster particle breakdown in the rumen allowing larger meal sizes before animals became constrained. The higher intakes of silage when animals consumed the SB supplement may be due to a slower rate of fermentation of the supplement, which was more closely matched to that of silage. Although not significant there was a tendency for differences in silage intake between animals receiving B compared with SB supplements to be greater for animals receiving the H silage suggesting that supplementation strategies to ensure optimal forage utilization may differ for silages of differing DM content.
Estimates of parental-dominance and full-sib permanent environment variances in laying hens
Authors: Misztal I. , Besbes B. 
Published: August 2016

AbstractEstimates of variance components for five egg traits on 26265 laying hens were obtained by restricted maximum likelihood (REML) using several models. In the DOMFS model, the effects included hatch group, additive genetic, full-sib, parental dominance and inbreeding depression. In the DOM model, the full-sib effect was eliminated. In the FS model, the parental dominance effect was eliminated. In the ADD model, both the full-sib and the dominance effects were eliminated. In the DOMFS model, the estimates of the full-sib variance were generally higher for egg production traits and lower for egg characteristics than those of the parental dominance variance. However, this model has partially failed in separating these two components. When the full-sib effect was removed from the model, almost all of its estimated variance moved to the estimated parental dominance variance. When the parental dominance effect was removed from the model, almost all of its estimated variance moved to the estimated full-sib variance. The estimates obtained with REML and the DOM model were compared with those obtained by method R and tilde-hat methodologies. The d2 (ratio of dominance variance to total variance) differed by up to 86% for method R and up to 225% for tilde-hat. The h2 differed by up to 26 and 28%, respectively. For data sets that are too large to be analysed with REML, method R is a feasible alternative. A model for estimation of dominance variance should also include the full-sib or a similar effect, provided the data set is large. Similarly, to analyse egg production traits, the model should include at least the full-sib effect.
Analysis of censored survival data using random regression models
Authors: Veerkamp R. F. , Brotherstone S. , Engel B. , Meuwissen T. H. E. 
Published: August 2016

AbstractCensoring of records is a problem in the prediction of breeding values for longevity, because breeding values are required before actual lifespan is known. In this study we investigated the use of random regression models to analyse survival data, because this method combines some of the advantages of a multitrait approach and the more sophisticated proportional hazards models. A model was derived for the binary representation of survival data and links with proportional hazards models and generalized linear models are shown. Variance components and breeding values were predicted using a linear approximation, including time-dependent fixed effects and random regression coefficients. Production records in lactations 1 to 5 were available on 24741 cows in the UK, all having had the opportunity to survive five lactations. The random regression model contained a linear regression on milk yield within herd (no. = 1417) by lactation number (no. = 4), Holstein percentage and year-month of calving effect (no. = 72). The additive animal genetic effects were modelled using orthogonal polynomials of order 1 to 4 with random coefficients and the error terms were fitted for each lactation separately, either correlated or not. Variance components from the full (i.e. uncensored) data set, were used to predict breeding values for survival in each lactation from both uncensored and randomly censored data. In the uncensored data, estimates of heritabilities for culling probability in each lactation ranged from 0·02 to 0·04. Breeding values for lifespan (calculated from the survival breeding values) had a range of 2·4 to 3·6 lactations and a standard deviation of 0·25. Correlations between predicted breeding values for 129 bulls, each with more than 30 daughters, from the various data sets ranged from 0·81 to 0·99 and were insensitive to the model used. It is concluded that random regression analysis models used for test-day records analysis of milk yield, might also be of use in the analysis of censored survival data.
The effect of oestrous cycle number, at constant age, on gilt reproduction in a dynamic service system
Authors: Grigoriadis D. F. , Edwards S. A. , English P. R. , Davidson F. 
Published: August 2016

AbstractPrevious studies examining the effect of age on gilt reproduction have generally failed to discriminate between chronological (i.e. days) and physiological age (i.e. number of oestrous cycles experienced) and the importance of each factor remains unclear in the pig. The present investigation tested the hypothesis that the physiological age at which gilts are bred in a dynamic service system (DSS) affects their reproductive behaviour and performance. A hundred and ninety gilts were randomly allocated between two treatments. They were introduced into the service pens of a DSS to be bred at the same age (215 days) but either at second (2H; no. = 96) or third (3H;no. = 94) post-pubertal heat period. The sexual behaviour of half of them was continuously recorded over a 13-week period and reproductive data from all the gilts were collected. Gilts of 2H treatment were significantly heavier at entry into the service pens (123 v. 119 kg; P < 0·05) than 3H gilts, but there was no significant difference in their backfat thickness. Treatment failed to affect the quality and frequency of mating attempts (MAs) gilts received or oestrus duration. Conception rate to first oestrus in the DSS (82%) was not affected by treatment. Litter size and number of piglets born alive was similar for 3H and 2H gilts (12·0 v. 11·7, s.e.d. 0·40 and 11·5 v. 11·0, s.e.d. 0·4 respectively; all P > 0·05). It is concluded that the reproductive behaviour and performance of gilts that had experienced either two or three oestrous cycles prior to mating at a constant age does not differ significantly in a DSS. More studies are needed in order to clarify the possible interactive influence of repeated and frequent services, which are common in a DSS, on gilt reproduction.
Seasonal changes in reproductive and pelage status during the initial ‘quiescent’ and first ‘active’ breeding seasons of the peripubertal pony colt
Authors: Argo C. McG. , Collingsworth M.G. R. , Cox J. E. 
Published: August 2016

AbstractPony colts (~12 m) are generally infertile during the breeding season of mature stallions. The mechanism which ‘delays’ puberty beyond the first potential breeding opportunity has not been described. Four pre- and four post-pubertal colts were monitored from November for 13 (group 1, 6 to 19 months of age) and 9 months (group 2, 18 to 27 months of age). Fortnightly, 15 blood samples were collected at hourly intervals from each colt to determine concentrations of prolactin (PRL), LH, FSH and testosterone (T). Testicular biopsies were collected monthly to evaluate spermatogenic activity. Puberty occurred between 17 and 19 months, and at the onset of the 2-year-old breeding season, all colts were fertile. Seasonal changes in the concentrations of all four hormones were observed in both groups. Prolactin concentrations were correlated with changes in day length (r = 0·88) in both groups, but a biphasic profile was noted in group 1. Moulting of the winter coat was coincident with seasonally increased PRL concentrations (P < 0·001). Seasonal changes in FSH, LH and T concentrations were similarly timed between groups and levels were maximal in mid April, mid March and mid May, respectively. However, overall mean concentrations of LH and T in group 1 animals were only 0·33 and 0·22 of group 2 values (P < 0·001). Suppression of gonadal activity during the colts’ first ‘quiescent’ breeding season was associated with low concentrations of circulating LH. Appropriately timed changes in plasma PRL, FSH and T concentrations and pelage indicated that the photoperiodic mechanism was functional in the pre-pubertal colt but pituitary LH release may be blocked by immaturity or active suppression.
Observations on the pre- and post-weaning behaviour of piglets reared in commercial indoor and outdoor environments
Authors: Cox L. N. , Cooper J. J. 
Published: August 2016

AbstractA number of behavioural problems are associated with weaning piglets including belly nosing, ear and tail biting, and low intake of solid food. These appear to be less pronounced in piglets reared on outdoor systems, which initially consume more solid food and show less belly nosing and aggression than comparable indoor-reared piglets. The objective of this study was to investigate how these differences in post-weaning behaviour relate to the piglets’ pre-weaning behaviour in the two rearing environments. The study was carried out at a commercial pig unit, where piglets of the same genotype are born into conventional indoor or outdoor farrowing systems. In the intensive system, sows were singly housed prior to farrowing in crates and their piglets received a solid ‘creep’ food prior to weaning. On the outdoor system, sows were allowed to build straw nests in arks for farrowing and both sow and piglets had access to pasture. Indoor and outdoor piglets were weaned at 24 (±3) days of age and mixed in straw-yard housing with access to a solid food. Prior to weaning, teat-directed activity was more common in indoor piglets than outdoor piglets. Outdoor piglets performed more rooting, standing and locomotion and were seen chewing the sows’ roll-nuts. Following weaning, outdoor-reared piglets performed more feeding and rooting, and less fighting than indoor-reared piglets. This study supports previous findings that undesirable activities such as fighting are less common in piglets weaned from outdoor systems, even when mixed with indoor piglets. In addition, outdoor-reared piglets were more likely to exploit solid food even though they did not have access to creep food prior to weaning.
The farmers’ influence on calves’ behaviour, health and production of a veal unit
Authors: Lensink B. J. , Veissier I. , Florand L. 
Published: August 2016

AbstractAlthough veal production is a highly standardized system, there still remains considerable variability in productivity between units. This variability might be due to the farmers working on these units, through differences in stockmanship, work attitudes or behaviour, which in turn may affect animals’ fear responses, productivity and health. A survey was conducted on 50 commercial farms affiliated to the same veal company. All units had calves housed in individual crates, a similar number of crates, and the same diets and management advice. Data were collected on: building and general farm characteristics, farmers’ backgrounds and their attitudes towards their work and calves, farmers’ behaviour with the calves, calves’ reactivity to people, and disease level of the calves. Productivity results (growth rates, food efficiency and mortality) were used to classify veal units as ‘high producing’ (no. = 24) v. ‘moderate producing’ (no. = 26). Calves were less reactive to people in units where the farmer behaved sympathetically and where there were several stockpersons working. The disease level was lower in units where the farmer had a positive attitude towards the sensitivity of the calves to contact and towards the importance of cleaning. Productivity of the veal unit was associated with the health of the calves but not with their reactivity to people.It is suggested that the farmer can have an influence on the success of the veal unit mainly through his/her ability to control the health of the calves and that a positive attitude towards animals and towards work (specifically cleaning actions) can improve the accuracy of surveillance and care of the animals. Moreover, through his/her behaviour with the calves and his/her ability to control their health, the farmer can play an important rôle in assuring calves’ welfare.
Growth and rumen digestion characteristics of steers grazing autumn grass supplemented with concentrates based on different carbohydrate sources
Authors: French P. , Moloney A. P. , O’Kiely P. , O’Riordan E. G. , Caffrey P. J. 
Published: August 2016

AbstractThe objective of this study was to determine the effects on animal performance and rumen digestion characteristics of supplementing cattle grazing autumn grass offered at two levels of allowance, with concentrates formulated from different carbohydrate sources. Seventy-two steers (494 (s.e. 38·4) kg) were assigned to one of six treatments which were arranged in a two grass allowances (5·5 or 11 kg grass dry matter (DM) per head per day) by three concentrate types (starch, starch + fibre or fibre-based diets) factorial design. The experiment commenced on 22 August and had a mean duration of 84 days. Concentrates were formulated to have similar metabolizable energy and crude protein and animals were offered 5 kg per head daily. Grass allowances were offered daily and concentrates were offered individually. Six rumen fistulated steers were used concurrently in a Latin square design to determine the effect of the diets on rumen digestion characteristics. Grass and concentrates degradabilities were measured using the nylon bag technique. The concentrate DM was degraded at 0·189, 0·102 and 0·094 per h for the starch, starch + fibre and fibre-based diets, respectively. Concentrate carbohydrate source and grass allowance did not affect (P> 0·05) degradation rate of grass DM or nitrogen in the rumen, rumen fluid pH, rumen fluid concentration of volatile fatty acids, total lactic acid or ammonia concentration or plasma concentration of glucose, urea or beta-hydroxybutyrate. Animals offered the low grass allowance had larger (P< 0·05) diurnal variation in the rumen fluid acetate: propionate ratio than those offered the high grass allowance. Increasing grass allowance increased (P< 0·01) carcass gain whereas concentrate type had no effect. There was no interaction between concentrate carbohydrate source and grass allowance on any of the production variables measured. It is concluded that the rumen environment in cattle grazing autumn grass buffered the effects of concentrate carbohydrate degradability such that similar productivity was achieved with each concentrate.