bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copper and Gold Cyclic (Alkyl)(amino)carbene Complexes with Sub‐Microsecond Photoemissions: Structure and Substituent Effects on Redox and Luminescent Properties
Authors: Romanov Alexander S. , Becker Ciaran R. , James Charlotte E. , Di Dawei , Credgington Dan , Linnolahti Mikko , Bochmann Manfred 
Published: February 2017

AbstractCopper and gold halide and pseudo‐halide complexes stabilised by methyl‐, ethyl‐ and adamantyl‐substituted cyclic (alkyl)(amino)carbene (CAAC) ligands are mostly linear monomers in the solid state, without aurophilic Au⋅⋅⋅Au interactions. (Et2L)CuCl shows the highest photoluminescence quantum yield (PLQY) in the series, 70 %. The photoemissions of Me2L and Et2L copper halide complexes show S1→S0 fluorescence on the ns time scale, in agreement with theory, as well as a long‐lived emission. Monomeric (Me2L)CuNCS is a white emitter, whereas dimeric [(Et2L)Cu(μ‐NCS)]2 shows intense yellow emission with a photoluminescence (PL) quantum yield of 49 %. The reaction of (AdL)MCl (M=Cu or Au) with phenols ArOH (Ar=Ph, 2,6‐F2C6H3, 2,6‐Me2C6H3, 3,5‐tBu2C6H3, 2‐tBu‐5‐MeC6H3, 2‐pyridyl), thiophenol, or aromatic amines H2NAr′′ (Ar′=Ph, 3,5‐(CF3)2C6H3, C6F5, 2‐py) afforded the corresponding phenolato, thiophenolato and amido complexes. Although the emission wavelengths are only marginally affected by the ring substitution pattern, the PL intensities respond sensitively to the presence of substituents in the ortho or meta positions. In gold aryloxides, PL is controlled by steric factors, with strong luminescence in compounds with Au‐O‐C‐C torsion angles <50°. Calculations confirm the dependence of oscillator strength on the torsion angle, as well as the inter‐ligand charge transfer nature of the emission. The HOMO/LUMO energy levels were estimated based on first reduction and oxidation potentials.
Asymmetric Synthesis of 2,3‐Dihydrobenzofurans by a [4+1] Annulation Between Ammonium Ylides and In Situ Generated o‐Quinone Methides
Authors: Meisinger Nicole , Roiser Lukas , Monkowius Uwe , Himmelsbach Markus , Robiette Raphaël , Waser Mario 
Published: February 2017

AbstractA highly enantio‐ and diastereoselective [4+1] annulation between in situ generated ammonium ylides and o‐quinone methides for the synthesis of a variety of 2,3‐dihydrobenzofurans has been developed. The key factors controlling the reactivity and stereoselectivity were systematically investigated by experimental and computational means and the energy profiles obtained provide a deeper insight into the mechanistic details of this reaction.
Microtubule‐regulating proteins and cAMP‐dependent signaling in neuroblastoma differentiation
Authors: Muñoz‐Llancao Pablo , de Gregorio Cristian , Las Heras Macarena , Meinohl Christopher , Noorman Kevin , Boddeke Erik , Cheng Xiaodong , Lezoualc'h Frank , Schmidt Martina , Gonzalez‐Billault Christian 
Published: February 2017

AbstractNeurons are highly differentiated cells responsible for the conduction and transmission of information in the nervous system. The proper function of a neuron relies on the compartmentalization of their intracellular domains. Differentiated neuroblastoma cells have been extensively used to study and understand the physiology and cell biology of neuronal cells. Here, we show that differentiation of N1E‐115 neuroblastoma cells is more pronounced upon exposure of a chemical analog of cyclic AMP (cAMP), db‐cAMP. We next analysed the expression of key microtubule‐regulating proteins in differentiated cells and the expression and activation of key cAMP players such as EPAC, PKA and AKAP79/150. Most of the microtubule‐promoting factors were up regulated during differentiation of N1E‐115 cells, while microtubule‐destabilizing proteins were down regulated. We observed an increase in tubulin post‐translational modifications related to microtubule stability. As expected, db‐cAMP increased PKA‐ and EPAC‐dependent signalling. Consistently, pharmacological modulation of EPAC activity instructed cell differentiation, number of neurites, and neurite length in N1E‐115 cells. Moreover, disruption of the PKA‐AKAP interaction reduced these morphometric parameters. Interestingly, PKA and EPAC act synergistically to induce neuronal differentiation in N1E‐115. Altogether these results show that the changes observed in the differentiation of N1E‐115 cells proceed by regulating several microtubule‐stabilizing factors, and the acquisition of a neuronal phenotype is a process involving concerted although independent functions of EPAC and PKA.
Colloidal Metal Nanoparticles Prepared by Laser Ablation and their Applications
Authors: Zhang Jianming , Claverie Jerome , Chaker Mohamed , Ma Dongling 
Published: February 2017

AbstractThis review article highlights the recent advances of the synthesis and application of metal nanoparticles (NPs) fabricated via pulsed laser ablation in liquid (PLAL) phase and also introduces relevant NP formation mechanisms. Although wet‐chemical approaches have been well established to synthesize colloidal metal NPs with various components and structures, some inherent drawbacks, such as reaction residuals and/or contaminations, largely limit some of their applications. The PLAL method has recently been developed as an alternative approach and received increasing attention for colloidal NP preparation, without involving complicated chemical reactions. In certain cases, by using PLAL, ligand‐free and surface‐clean NPs can be obtained and well dispersed in liquid, leading to the formation of a “surface‐clean” NP dispersion. This unique feature renders PLAL‐synthesised metal NPs attractive candidates for many interesting applications in catalysis, biology, sensing, and clean energy generation and storage. We conclude this review by proposing several interesting research directions and future challenges, from PLAL fabrication to applications. We hope this review can serve as a good reference and help with the further development of PLAL‐NPs and their diverse applications.
SILAR BiOI‐Sensitized TiO2 Films for Visible‐Light Photocatalytic Degradation of Rhodamine B and 4‐Chlorophenol
Authors: Odling Gylen , Robertson Neil 
Published: February 2017

AbstractBiOI nanoplates were deposited upon a film of TiO2 nanoparticles derived from a commercial source using a simple room temperature sequential ionic layer adsorption and reaction (SILAR) method. X‐ray diffraction, X‐ray photoelectron spectroscopy and electron microscopies have been used to confirm the crystal phase, chemical states of key elements and morphology of the BiOI nanoplate–TiO2 composites. Using both valence band X‐ray photoelectron spectroscopy and UV/Vis diffuse reflectance measurements the band structure of the composites is determined to be that of a type II heterojunction. Through initial screening of the photocatalytic activity of the SILAR‐modified films it was determined that five SILAR cycles are optimal in the photocatalytic degradation of rhodamine B. The visible‐light sensitisation effect of BiOI was then proven by examination of the photocatalytic degradation of the colourless organic pollutant 4‐chlorophenol, showing a large enhancement over an equivalent TiO2 film.
SaccharopolysporaSpecies: Laboratory Maintenance and Enhanced Production of Secondary Metabolites
Authors: Dhakal Dipesh , Pokhrel Anaya Raj , Jha Amit Kumar , Thuan Nguyen Huy , Sohng Jae Kyung 
Published: February 2017

AbstractSaccharopolysporaspp. are aerobic, Gram‐positive, non‐acid‐fast, and non‐motile actinomycetes. Various species of the genusSaccharopolysporahave been reported with an ability to produce various bioactive compounds for pharmaceutical and agricultural uses. This unit includes general protocols for the laboratory maintenance ofSaccharopolysporaspecies, including growth in liquid medium, growth on solid agar, long‐term storage, and generation of a higher producer strain by mutagenesis.Saccharopolyspora spinosaATCC 49460 is used as a prototype for explaining the considerations for efficient laboratory maintenance ofSaccharopolysporaspp.Saccharopolyspora spinosais a producer of spinosad, a prominent insecticide with selective activity against various insects. © 2017 by John Wiley & Sons, Inc.
Actinomadura Species: Laboratory Maintenance and Ribosome Engineering
Authors: Dhakal Dipesh , Chung Nguyen Thanh , Rayamajhi Vijay , Sohng Jae Kyung 
Published: February 2017

Abstract
Actinomadura spp. are aerobic, Gram‐positive, catalase‐positive, non‐acid fast, non‐motile actinomycetes. Some species of Actinomadura are associated with opportunistic infections in humans. However, many bioactive compounds with pharmaceutical applications can be isolated from various Actinomadura spp. This unit includes general protocols for the laboratory maintenance of Actinomadura spp., including growth in liquid medium, growth on solid agar, long‐term storage, and generation of a higher producing strain by ribosome engineering. Actinomadura hibisca P157‐2 is used as a prototype for explaining the considerations for efficient laboratory maintenance of Actinomadura spp. © 2017 by John Wiley & Sons, Inc.
Cryptosporidium: Identification and Genetic Typing
Authors: Šlapeta Jan 
Published: February 2017

Abstract
Cryptosporidium spp. are obligate protozoan parasites of the gastrointestinal tract of vertebrates, including humans. In the majority of human cases, the diarrheal disease cryptosporidiosis is caused by either the human‐adapted species Cryptosporidium hominis or the zoonotic Cryptosporidium parvum ‘bovine genotype’ (also known as Cryptosporidium pestis). The infectious stage, environmentally resilient Cryptosporidium oocysts, are shed by the infected host. Cryptosporidium parasites are transmitted by the fecal‐oral route and are one of the major water‐borne pathogens. The cryptic nature of the microscopic Cryptosporidium oocysts coupled with the existence of several host‐adapted and zoonotic species requires molecular tools to identify Cryptosporidium spp. in either fecal or environmental samples. This unit describes methods for Cryptosporidium identification and typing using genotyping based on nuclear loci. We also provide a protocol for morphological confirmation of Cryptosporidium oocysts based on antibody labeling of the Cryptosporidium oocyst wall and a protocol for purification of oocysts from fecal material. © 2017 by John Wiley & Sons, Inc.
Efficient Genome Editing in the Oomycete Phytophthora sojae Using CRISPR/Cas9
Authors: Fang Yufeng , Cui Linkai , Gu Biao , Arredondo Felipe , Tyler Brett M. 
Published: February 2017

Abstract
Phytophthora is a filamentous fungus‐like microorganism, but belongs to the oomycetes, in the kingdom Stramenopila. Phytophthora species are notorious as plant destroyers, causing multibillion‐dollar damage to agriculture and natural ecosystems worldwide annually. For a long time, genome editing has been unattainable in oomycetes, because of their extremely low rate of homologous recombination. The recent implementation of the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) system in the soybean pathogen Phytophthora sojae, an experimental model for oomycetes, has opened up a powerful new research capability for the oomycete community. Here, we describe a detailed protocol for CRISPR/Cas9‐mediated genome editing in P. sojae, including single guide RNA (sgRNA) design and construction, efficient gene replacement, and mutant‐screening strategies. This protocol should be generally applicable for most culturable oomycetes. We also describe an optimized transformation method that is useful for other Phytophthora spp. including P. capsici and P. parasitica. © 2017 by John Wiley & Sons, Inc.
Toxoplasma gondii: Laboratory Maintenance and Growth
Authors: Khan Asis , Grigg Michael E. 
Published: February 2017

Abstract
Toxoplasma gondii is a highly successful apicomplexan protozoan capable of infecting any warm‐blooded animal worldwide. In humans, Toxoplasma infections are life‐long, with approximately one‐third of the world's population chronically infected. Although normally controlled by the host immune system, T. gondii infection can lead to a variety of clinical outcomes in individuals with immature or suppressed immune systems. After penetrating the intestine, parasites rapidly disseminate throughout the body and stimulate production of the cytokines interleukin (IL)‐12, IL‐18, and interferon (IFN)‐γ by immune cells. These cytokines play a key role in host resistance to T. gondii by promoting a strong Th1 response. Recent reports show that gut commensal bacteria can act as molecular adjuvants during T. gondii infection. Thus, T. gondii is an excellent model system to study host‐pathogen interactions. This unit outlines the protocols for in vitro and in vivo maintenance and growth of T. gondii. © 2017 by John Wiley & Sons, Inc.
Supported Molybdenum Catalysts for the Deoxydehydration of 1,4‐Anhydroerythritol into 2,5‐Dihydrofuran
Authors: Sandbrink Lennart , Beckerle Klaus , Meiners Isabell , Liffmann Rebecca , Rahimi Khosrow , Okuda Jun , Palkovits Regina 
Published: February 2017

AbstractEfficient deoxygenation strategies are crucial for the valorization of renewable feedstocks. Deoxydehydration (DODH) enables the direct transformation of two adjacent hydroxyl groups into a double bond. Supported molybdenum‐based catalysts were utilized for the first time in DODH. MoOx/TiO2 showed superior catalytic activity compared to common molybdenum salts. The catalyst efficiently converted 1,4‐anhydroerythritol into 2,5‐dihydrofuran in the presence of 3‐octanol as reducing agent, showing high reproducibility and stability.
An efficient chaos‐based 2‐party key agreement protocol with provable security
Authors: Luo Min , Zhang Yunru , Khan Muhammad Khurram , He Debiao 
Published: February 2017

SummaryKey agreement protocol is an important cryptographic primitive, which allows 2 parties to establish a secure session in an open network environment. A various of key agreement protocols were proposed. Nowadays, there still exists some other security flaws waiting to be solved. Owing to reduce the computational and communication costs and improve the security, chaotic map has been studied in‐depth and treated as a good solution. Recently, Liu et al proposed a chaos‐based 2‐party key agreement protocol and demonstrated that it can defend denial‐of‐service attack and replay attack. We found, however, it cannot resist off‐line password‐guessing attack, and it also has some other security flaws. In this paper, we propose an improved chaos‐based 2‐party key agreement protocol. The results prove that the protocol can solve the threats of off‐line password‐guessing attack and other security flaws in the security proof section. What is more, performance analysis shows that the computational cost of the improved protocol is lower than Liu et al protocol.
Species identification and comparative population genetics of four coastal houndsharks based on novel NGS‐mined microsatellites
Authors: Maduna Simo N. , Rossouw Charné , da Silva Charlene , Soekoe Michelle , Bester‐van der Merwe Aletta E. 
Published: February 2017

AbstractThe common smooth‐hound (Mustelus mustelus) is the topmost bio‐economically and recreationally important shark species in southern Africa, western Africa, and Mediterranean Sea. Here, we used the Illumina HiSeq™ 2000 next‐generation sequencing (NGS) technology to develop novel microsatellite markers forMustelus mustelus. Two microsatellite multiplex panels were constructed from 11 polymorphic loci and characterized in two populations ofMustelus mustelusrepresentative of its South African distribution. The markers were then tested for cross‐species utility inGaleorhinus galeus,Mustelus palumbes, andTriakis megalopterus, three other demersal coastal sharks also subjected to recreational and/or commercial fishery pressures in South Africa. We assessed genetic diversity (NA,AR,HO,HE,and PIC) and differentiation (FSTandDest) for each species and also examined the potential use of these markers in species assignment. In each of the four species, all 11 microsatellites were variable with up to a meanNAof 8,ARup to 7.5,HEand PIC as high as 0.842. We were able to reject genetic homogeneity for all species investigated here except forT.megalopterus. We found that the panel of the microsatellite markers developed in this study could discriminate between the study species, particularly for those that are morphologically very similar. Our study provides molecular tools to address ecological and evolutionary questions vital to the conservation and management of these locally and globally exploited shark species.
Candidate susceptibility variants for esophageal squamous cell carcinoma
Authors: Donner Iikki , Katainen Riku , Tanskanen Tomas , Kaasinen Eevi , Aavikko Mervi , Ovaska Kristian , Artama Miia , Pukkala Eero , Aaltonen Lauri A. 
Published: February 2017

AbstractEsophageal cancer is common worldwide, and often fatal. The major histological subtype is esophageal squamous cell carcinoma (ESCC). ESCC shows familial aggregation and high heritability. Mutations in RHBDF2 cause tylosis, a very rare disorder characterized by high life‐time risk of ESCC, but no other well‐established predisposition genes have been identified. To identify candidate susceptibility variants for ESCC we utilized the Population Information System and the Finnish cancer registry to find study materials by clustering ESCC patients by family name at birth and municipality at birth. We collected archival tissue material and exome sequenced a total of 30 ESCC cases. We prioritized shared, deleterious and rare variants that were significantly enriched in our sample set compared to Finnish and population subset specific controls. Six variants passed filtering, the most frequent being a nonsense mutation in DNAH9 (p.Tyr1573Ter) found in four unrelated patients. DNAH9 has been reported to be frequently lost in ESCC tumors. In this study, one patient's tumor showed loss of the wild type allele of DNAH9 suggesting a tumor suppressive function. A missense variant in GKAP1 was shared by three patients, and missense variants in BAG1, NFX1, FUK, and DDOST by two each. EP300 which has previously been implicated in the genesis of ESCC had a missense variant segregating in three affected individuals in a single family. If validated in independent patient sets, these variants could serve as a tool towards prevention and early diagnosis of ESCC.
HIF1A gene polymorphisms and human diseases: Graphical review of 97 association studies
Authors: Gladek I. , Ferdin J. , Horvat S. , Calin G. A. , Kunej T. 
Published: February 2017

AbstractHypoxia‐inducible factors (HIFs) belong to a family of transcription factors (TF) responsive to a low O2 availability, which is often a characteristic feature of solid tumors. The alpha subunit of the HIF heterodimer is O2‐sensitive, and once stabilized in hypoxia, it functions as a master regulator of various genes involved in hypoxia pathway. Changes in the HIF1A (hypoxia inducible factor 1, alpha subunit) nucleotide sequence or expression has been shown to be associated with the development of several diseases. Because of increasing research interest in HIF1A gene a review of association studies was needed. We here reviewed published data on single nucleotide polymorphisms (SNPs) in HIF1A in various diseases; in total, 34 SNPs were tested for an association with 49 phenotypes, and the results were visualized using the Cytoscape software. Among all collected polymorphisms 16 SNPs showed significant associations with 40 different phenotypes, including six SNPs associated with 14 cancer types. Missense SNPs (rs11549465 and rs11549467) within the oxygen‐dependent degradation domain were most frequently studied. The study provides a comprehensive tool for researchers working in this area and may contribute to more accurate disease diagnosis and identification of therapeutic targets.
Counselling for deinfibulation among women with type III female genital mutilation: A systematic review
Authors: Bello Segun , Ogugbue Miriam , Chibuzor Moriam , Okomo Uduak , Meremikwu Martin M. 
Published: February 2017

AbstractBackgroundCounselling is a routine practice done before deinfibulation in women with type III female genital mutilation (FGM). However, cultural and social pressures, in addition to maladaptation to the changes in the body post deinfibulation, cause some women to choose to be reinfibulated after being deinfibulated.ObjectiveTo conduct a systematic review of the impact of counselling prior to deinfibulation on patient satisfaction, marital satisfaction, and rate of requests for reinfibulation among women living with type III FGM. The secondary aim was to assess the impact of male partner involvement in counselling on patient satisfaction, marital satisfaction, and rate of requests for reinfibulation.Search strategyMajor databases including Cochrane Central Register of Controlled Trials, Medline, SCOPUS, and ClinicalTrials.gov were searched until August 2015.Selection criteriaStudies comparing women with type III FGM who received counselling before deinfibulation versus no counselling were included.Data collection and analysisTwo team members independently screened and collected data.ResultsNo eligible studies were identified.ConclusionThere is no evidence to conclude that counselling before deinfibulation influences patients’ satisfaction with overall quality of care or rates of request for reinfibulation.PROSPERO registrationCRD42015024675.
Deinfibulation for treating urologic complications of type III female genital mutilation: A systematic review
Authors: Effa Emmanuel , Ojo Olumuyiwa , Ihesie Austin , Meremikwu Martin M. 
Published: February 2017

AbstractBackgroundWomen and girls who have undergone type III female genital mutilation (FGM) may suffer urologic complications such as recurrent urinary tract infections, obstruction, stones, and incontinence.ObjectiveTo assess the effectiveness of deinfibulation for preventing and treating urologic complications in women and girls living with FGM.Search strategyThe following major databases were searched from inception to August 2015: Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, SCOPUS, Web of Science, and ClinicalTrials.gov without language restrictions.Selection criteriaRandomized controlled studies (RCTs) or observational studies with controls were considered.Data collection and analysisWe screened the results of the search independently for potentially relevant studies and applied inclusion and exclusion criteria for the full texts of the relevant studies.ResultsNo RCTs were found. We found three case reports and a retrospective case review, all of which were excluded.ConclusionThere is no evidence on the use of deinfibulation to improve urologic complications among women with type III FGM. Current clinical practice may be informed by anecdotal evidence from case reports. Appropriate RCTs and observational studies with comparison groups in countries where FGM is common are needed.PROSPERO registration: CRD42015024901.