bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subtle Changes in Clonal Dynamics Underlie the Age-Related Decline in Neurogenesis
Authors: Bast Lisa , Calzolari Filippo , Strasser Michael , Hasenauer Jan , Theis Fabian , Ninkovic Jovica , Marr Carsten 
Published: October 2017

SUMMARYNeural stem cells in the adult murine brain have only a limited capacity to self-renew, and the number of neurons they generate drastically declines with age. How cellular dynamics sustain neurogenesis and how alterations with age may result in this decline, are both unresolved issues. Therefore, we clonally traced neural stem cell lineages using confetti reporters in young and middle-aged adult mice. To understand underlying mechanisms, we derived mathematical population models of adult neurogenesis that explain the observed clonal cell type abundances. Models fitting the data best consistently show self renewal of transit amplifying progenitors and rapid neuroblast cell cycle exit. Most importantly, we identified an increase of asymmetric stem cell divisions at the expense of symmetric stem cell differentiation with age. Beyond explaining existing longitudinal population data, our model identifies a particular cellular strategy underlying adult neural stem cell homeostasis that gives insights into the aging of a stem cell compartment.
Balancing Strengths and Weaknesses in Dimensional Psychiatry
Authors: Alexander Lindsay M. , Salum Giovanni A. , Swanson James M. , Milham Michael P. 
Published: October 2017

ABSTRACTObjectiveTo evaluate the feasibility and value of creating an extensible framework for psychiatric phenotyping that indexes both strengths and weaknesses of behavioral dimensions. The Extended Strengths and Weaknesses Assessment of Normal Behavior (E-SWAN) reconceptualizes each diagnostic criterion for selected DSM-5 disorders as a behavior, which can range from high (strengths) to low (weaknesses). Initial efforts have focused on Panic Disorder, Social Anxiety, Major Depression, and Disruptive Mood Dysregulation Disorder.MethodsData were collected from 523 participants (ages: 5-21 years old) in the Child Mind Institute Healthy Brain Network − an ongoing community-referred study. Parents completed each of the four E-SWAN scales and traditional unidirectional scales addressing the same disorders. Distributional properties, Item Response Theory Analysis (IRT) and Receiver Operating Characteristic (ROC) curves (for diagnostic prediction) were used to assess and compare the performance of E-SWAN and traditional scales.ResultsIn contrast to the traditional scales, which exhibited truncated distributions, all four E-SWAN scales were found to have near-normal distributions. IRT analyses indicate the E-SWAN subscales provided reliable information about respondents throughout the population distribution; in contrast, traditional scales only provided reliable information about respondents at the high end of the distribution. Predictive value for DSM-5 diagnoses was comparable to prior scales.ConclusionE-SWAN bidirectional scales can capture the full spectrum of the population distribution for DSM disorders. The additional information provided can better inform examination of inter-individual variation in population studies, as well as facilitate the identification of factors related to resiliency in clinical samples.
Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy
Authors: Ingargiola Antonino , Segal Maya , Gulinatti Angelo , Rech Ivan , Labanca Ivan , Maccagnani Piera , Ghioni Massimo , Weiss Shimon , Michalet Xavier 
Published: October 2017

AbstractSingle-molecule fluorescence spectroscopy (SMFS), based on the detection of individual molecules freely diffusing through the excitation spot of a confocal microscope, has allowed unprecedented insights into biological processes at the molecular level, but suffers from limited throughput. We have recently introduced a multispot version of SMFS, which allows achieving high-throughput SMFS by virtue of parallelization, and relies on custom silicon single-photon avalanche diode (SPAD) detector arrays. Here, we examine the premise of this parallelization approach, which is that data acquired from different spots is uncorrelated. In particular, we measure the optical crosstalk characteristics of the two 48-SPAD arrays used in our recent SMFS studies, and demonstrate that it is negligible (crosstalk probability ≤ 1.1 10−3) and is undetectable in cross-correlation analysis of actual single-molecule fluorescence data.
Specific hippocampal representations are linked to generalized cortical representations in memory
Authors: Yu Jai Y. , Liu Daniel F. , Loback Adrianna , Grossrubatscher Irene , Frank Loren M. 
Published: October 2017

SummaryMemories link information about specific experiences to more general knowledge that is abstracted from and contextualizes those experiences, but how neuronal activity patterns support this link is not known. Here we show that during memory reactivation in a foraging task with multiple spatial paths, specific hippocampal place representations are concurrently and preferentially reactivated with a subset of prefrontal cortical task representations that generalize across different paths. This link between specific and general representations may serve as a neural substrate for abstraction and task guidance in mammals.
Signaling Networks and Dynamics of Allosteric Transitions in Bacterial Chaperonin GroEL: Implications for Iterative Annealing of Misfolded Proteins
Authors: Thirumalai D. , Hyeon Changbong 
Published: October 2017

AbstractSignal transmission at the molecular level in many biological complexes occurs through allosteric transitions. They describe the response a complex to binding of ligands at sites that are spatially well separated from the binding region. We describe the Structural Perturbation Method (SPM), based on phonon propagation in solids, that can be used to determine the signal transmitting allostery wiring diagram (AWD) in large but finite-sized biological complexes. Applications to the bacterial chaperonin GroEL-GroES complex shows that the AWD determined from structures also drive the allosteric transitions dynamically. Both from a structural and dynamical perspective these transitions are largely determined by formation and rupture of salt-bridges. The molecular description of allostery in GroEL provides insights into its function, which is quantitatively described by the Iterative Annealing Mechanism. Remarkably, in this complex molecular machine, a deep connection is established between the structures, reaction cycle during which GroEL undergoes a sequence of allosteric transitions, and function in a self-consistent manner.
High contrast staining for serial block face scanning electron microscopy without uranyl acetate
Authors: Odriozola Adolfo , Llodrá Jaime , Radecke Julika , Ruegsegger Céline , Tschanz Stefan , Saxena Smita , Stephan Rohr , Zuber Benoît 
Published: October 2017

1AbstractSerial block face scanning electron microscopy (SBFSEM) is an increasingly popular method for investigating the three-dimensional ultrastructure of large biological samples. Prior to imaging, samples are typically chemically fixed, stained with osmium and uranyl acetate, and subsequently embedded in resin. The purpose of staining is to provide image contrast and reduce specimen charging under the electron beam, which is detrimental to the quality of imaging. Obtaining, using, and disposing of uranyl acetate is getting increasingly cumbersome in many countries due to new regulations on the handling of radioactive substances. Therefore, we developed an alternative staining procedure that does not rely on the use of uranium or any other radioactive substance. This procedure provides excellent contrast and efficiently reduces specimen charging.
Networking and development idiosyncratic deals
Authors: Guerrero Sylvie , Jeanblanc Hélène Challiol 
Published: October 2017

PurposeThe purpose of this paper is to explore the antecedents of development idiosyncratic deals (i-deals) from an organizational politics and a situationist perspective. The paper tests a moderated mediation model in which networking skills is indirectly related to development i-deal in a context of high hierarchical plateau.Design/methodology/approachThe authors test the research model with a sample of 252 engineers, 88 percent male, who work in an economically wealthy region of France and who are thus well positioned to negotiate development i-deals.FindingsThe authors lead analyses with the Preacheret al.’s macros on SPSS. Results support the hypotheses. The authors find that support-seeking behaviors partially mediate the relationship between networking skills and development i-deals, and that this relationship is significant only in a context of high plateauing.Originality/valueOverall, this study contributes to a deeper understanding of i-deal antecedents by bridging the literatures on i-deals and careers. It also shows that socially skilled employees are able to seek support and in turn, to proactively negotiate development i-deals. This process is a way to cope with perceptions of hierarchical plateau.
Should we talk? Co-rumination and conversation avoidance in job search
Authors: Haggard Dana L. , da Motta Veiga Serge P. , LaPreze Melody W. 
Published: October 2017

PurposeThe purpose of this paper is to adopt an approach/avoidance coping framework to examine the relationships of job search co-rumination (i.e. engaging in repeated and excessive conversations with a friend about job search problems) and job search talk avoidance (i.e. persistently seeking to escape conversations about the job search) on job search intensity and job search procrastination.Design/methodology/approachThe authors surveyed 196 new labor market entrants (i.e. graduating students) at two points in time during their last semester in college.FindingsThe authors found that job search co-rumination is positively related to job search intensity, while job search talk avoidance is positively related to job search procrastination. Interestingly, though, the expected negative relationships between job search co-rumination and job search procrastination and between job search talk avoidance and job search intensity were not significant.Practical implicationsThis study has implications for both job seekers and career counselors. For job seekers, understanding how their communication patterns influence their behaviors (and ultimately their success) can help them to see the benefits of a balanced approach to sharing about their job search. Furthermore, career centers could organize either job search mentoring or peer group programs to help job seekers navigate the intricacies of the job search process.Originality/valueThis study contributes to understanding whether and how talking (or not) with others (i.e. friends and relatives) about one’s job search influences one’s job search behaviors, such as intensity and procrastination.
Enhancing the contribution of volunteering to career commitment with friendship among university students
Authors: Cheung Chau-kiu , Liu Elaine Suk-ching 
Published: October 2017

Purpose
Encouraging college students to volunteer is a supposed but uncharted way to contribute to their career commitment. Clarifying the ways of the contribution is therefore necessary. From the social capital perspective, volunteering and network density among friends represent social capital to reinforce each other. Thus, the purpose of this paper is to study the enhancement of the contribution by the density.

Design/methodology/approach
The examination employs a two-wave panel survey of 410 university students to estimate the effects of volunteering and friend network density at Wave 1 on career commitment at Wave 2. Essentially, the examination adjusted for biases due to sample attrition and self-selection into volunteering.

Findings
Volunteering at Wave 1 showed a significant contribution to career commitment at Wave 2. Moreover, the contribution significantly increased with friend network density at Wave 1.

Research limitations/implications
Findings from this panel survey of university students in Hong Kong require future research for substantiation. For instance, such research can apply an experimental design to volunteering to guarantee the internal validity of the contribution of volunteering.

Practical implications
Social capital theory is applicable to the promotion of career commitment. Specifically, optimizing the strength of social capital through the combination of volunteering and friendship is promising.

Originality/value
Empirical support for the application of social capital theory to career development is evident. Particularly, the joint contribution of volunteering and friendship is demonstrable.
Understanding and addressing Islamophobia in organizational settings
Authors: Sekerka Leslie E. , Marar Yacobian Marianne 
Published: October 2017

PurposeThe purpose of this paper is to call to public leaders to exercise moral courage in choosing to understand and address phobic biases and prejudicial attitudes toward Muslims in the workplace. With reference to developments in the USA, workplace discrimination is framed as an ethical issue, with Islamophobia viewed as a rapidly growing concern.Design/methodology/approachThis work is a practical application of existing theory and research in positive organizational scholarship to address the concern of workplace discrimination; specifically Islamophobia. Propositions are developed to depict how public leaders can address Islamophobia and other forms of discrimination by role modeling moral courage.FindingsThe findings show that Islamophobia is an ethical challenge for public leaders, one that can begin to be addressed by exercising character strength that promotes tolerance, civility, and respect. This proactive approach will enable public leaders to serve as pillars of openness, inclusion, and thoughtful regard for others, regardless of organizational members’ faith or culture.Social implicationsThe social implications are to encourage discourse among global public leaders, prompting awareness and concern for Islamophobia and promoting more informed paths for productive scholarship.Originality/valueStudies of workplace discrimination typically focus on race and gender, with few considering how Muslims face increasing Islamophobia. This work adds value to the existing literature by explicitly encouraging public leaders to respond, rather than react, to discrimination with moral competency.
Empirical analysis of how risk management by Korea’s exporting companies affects export performance
Authors: Joo Se-Hwan , Pak Myong-Sop 
Published: October 2017

Purpose
The purposes of this paper are as follows. First, the paper investigates the causes of risk and methods for managing it based on previous studies of trade risk and trade risk management. Second, the paper analyses the types and forms of trade risk for exporting companies and investigate the relationship between actual trade risks and perceptions of trade risk. Third, the paper establishes a measurement device for trade risk management and export performance based on previous studies. Fourth, the paper derives the concepts based on the accumulated details to establish a research model and verifies a cause and effect relationship. Fifth, the paper analyses what kind of effect the perception of trade risk exerts on trade risk management. And sixth, the paper analyses the effect of the method of trade risk management on the export performance of exporting companies to shed light on the utility of trade risk management.

Design/methodology/approach
The purpose in this research is to analyse the effects of trade risk management on the export performance of exporting companies. The authors have conducted a review of previous studies about trade risk, trade risk management, and the outcomes thereof. Based on that review, the authors have established a research model, derived hypotheses, and used statistical methods to verify those hypotheses.

Findings
First, the authors analysed the methods of settling payments, transaction terms, the transportation environment, and experience in trade claims and found that they influenced the perceived level of trade risk. Second, exporting companies’ prior perception of trade risk determines which methods of trade risk management are suitable. Third, the analysis of the methods of trade risk management and export performance found that financial performance was influenced more than non-financial performance by trade risk management.

Originality/value
The authors determined whether trade risk management effectively counters the losses incurred as a result of the trade risks faced by exporting countries. The authors used an empirical statistical analysis to comprehensively analyse appropriate trade risk management and export performance. Prior to implementing the empirical analysis, the authors conducted research on trade risk and its management and established a research model and research hypotheses based on a theoretical background of trade risk methods appropriate to the circumstances faced by exporting companies.
Effect of non-tariff measures on international tea trades
Authors: Hwang Chae Won , Lim Song Soo 
Published: October 2017

Purpose
The purpose of this paper is to analyze the impacts of differences in sanitary and phytosanitary measures as non-tariff measures (NTMs) in the tea trade between importing and exporting countries. With the progress of trade liberalization, there has been a shift of focus to NTMs as alternative or potential trade barriers.

Design/methodology/approach
In order to quantify an NTM on tea trade and implement its empirical application, this study designed an index of differences in maximum residue levels (MRLs) for the pesticide endosulfan and introduced it into a gravity trade model. The estimation challenges in the presence of heteroscedasticity and many zero-trade flows are resolved by taking the Heckman and Poisson pseudo-maximum likelihood estimators.

Findings
This study found that differences in MRLs, arising from the stricter standards in importing countries lead to a significant decrease in tea trade value. This negative impact of differences in MRLs is found to be slightly less than that of tariffs, implying that in this case, the NTM acts as a policy substitute for import tariffs in the global tea trade.

Originality/value
The main contribution of this study is to suggest and quantify the differences in MRLs across countries as a substantial NTM on the global tea trade and provide its empirical application.
Utilising a scholarship program as a workforce strategy for the community-managed mental health sector
Authors: Foreman Emma , McMillan Sara , Wheeler Amanda 
Published: October 2017

PurposeThe community-managed mental health sector needs to meet growing workforce demands. Yet, limited research has explored professional development opportunities and effective recruitment and retention strategies to support sector growth. One strategy is the use of a scholarship program to increase skills and training, via a University qualification. The purpose of this paper is to explore the progress of 19 mental health scholarship students and the impact of the scholarship on career intentions.Design/methodology/approachA mixed-methods approach comprising scholarship applications, questionnaires and semi-structured interviews was used to explore the students’ university experiences between February 2013 and January 2015. Course convenors of the Mental Health Program were interviewed. Data were collected across three time-points over 24 months, with each collection informing the next research phase. Data analysis involved thematic analysis and descriptive statistics.FindingsDeeper knowledge, recognition of experience, new career pathways and improved work practice were benefits. Managing time and study, and work-life balance were the greatest challenges. Completing students displayed a range of internal attributes and accessed external supports. At the time of the study, the scholarships maintained student motivation and intention to work in the sector.Originality/valueThis research provides a deeper understanding of the demographics of the sector’s workforce. Insight into the attributes of completing students was obtained. The benefits realized and the challenges faced by the scholarship recipients will inform ongoing workforce development programs for the community-managed mental health sector.
CRISPR/Cas9‐mediated PBP1 and PBP3 mutagenesis induced significant reduction in electrophysiological response to sex pheromones in male Chilo suppressalis
Authors: Dong Xiao‐Tong , Liao Hui , Zhu Guan‐Heng , Khuhro Sajjad Ali , Ye Zhan‐Feng , Yan Qi , Dong Shuang‐Lin 
Published: October 2017

AbstractPheromone‐binding proteins (PBPs) are thought to bind and transport sex pheromones onto the olfactory receptors on the dendrite membrane of olfactory neurons, and thus play a vital role in sex pheromone perception. However, the function of PBPs has rarely been demonstrated in vivo. In this study, two PBPs (PBP1 and PBP3) of Chilo suppressalis, one of the most notorious pyralid pests, were in vivo functionally characterized using insects with the PBP gene knocked out by the CRISPR/Cas9 system. First, through direct injection of PBP‐single guide RNA (sgRNA)/Cas9 messenger RNA into newly laid eggs, a high rate of target‐gene editing (checked with polled eggs) was induced at 24 h after injection, 21.3% for PBP1‐sgRNA injected eggs and 19.5% for PBP3‐sgRNA injected eggs. Second, by an in‐crossing strategy, insects with mutant PBP1 or PBP3 (both with a premature stop codon) were screened, and homozygous mutants were obtained in the G3 generation. Third, the mutant insects were measured for electroantennogram (EAG) response to female sex pheromones. As a result, both PBP mutant males displayed significant reduction in EAG response, and this reduction in PBP1 mutants was higher than that in PBP3 mutants, indicating a more important role of PBP1. Finally, the relative importance of two PBPs and the possible off target effect induced by sgRNA‐injection are discussed. Taken together, our study provides a deeper insight into the function of and interaction between different PBP genes in sex pheromone perception of C. suppressalis, as well as a valuable reference in methodology for gene functional study in other genes and other moth species.
A novel risk score for severe ARDS patients undergoing ECMO after retrieval from peripheral hospitals
Authors: Lazzeri C. , Cianchi G. , Mauri T. , Pesenti A. , Bonizzoli M. , Batacchi S. , Chiostri M. , Socci F. , Peris A. 
Published: October 2017

BackgroundExtracorporeal Membrane Oxygenation in severe ARDS unresponsive to conventional protective ventilation is associated with elevated costs, resource and complications, and appropriate risk stratification of candidate patients could be useful to recognize those more likely to benefit from ECMO. We aimed to derive a new outcome prediction score for patients retrieved by our ECMO team from peripheral centers, including systematic echocardiographic evaluation before ECMO start.MethodsSixty‐nine consecutive patients with refractory ARDS requiring ECMO transferred from peripheral centers to our ICU (a tertiary ECMO referral center), from 1 October 2009 to 31 December 2015, were assessed.ResultsAll patients were transported on ECMO (distance, median 77, range 4–456 km) The mortality rate was 41% (28/69). Our new risk score included age ≥ 42 years, BMI < 31 kg/m2, RV dilatation, and pH < 7.35. The proposed cut off (Youden's index method) of nine had a sensitivity of 96% and a specificity of 30% (AUC‐ROC: 0.85, 95% CI: 0.76–0.94, P < 0.001). When assessing the discriminatory ability of our risk score in the population of local patients, survivors had a mean value of 15.4 ± 8.6, whereas non‐survivors showed a mean value of 20.1 ± 7.4 (P < 0.001).ConclusionsOur new risk score shows good discriminatory ability both in patients retrieved from peripheral centers and in those implanted at our center. This score includes variables easily available at bedside, and, for the first time, a pathophysiologic element, RV dilatation.