bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquitination of LATS1
Authors: Ni Wen , Zhang Yuqin , Zhan Zetao , Ye Feng , Liang Yonghao , Huang Jing , Chen Keli , Chen Longhua , Ding Yi 
Published: April 2017

Abstract
Background
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, and tumor recurrence and metastasis are major factors that contribute to the poor outcome of patients with HCC. Long noncoding RNAs (lncRNAs) are known to regulate different tumorigenic processes, and a growing body of evidence indicates that Hippo kinase signaling is inactivated in many cancers. However, the upstream lncRNA regulators of Hippo kinase signaling in HCC are poorly understood.

Methods
Using a lncRNA microarray, we identified a novel lncRNA, uc.134, whose expression was significantly decreased in the highly aggressive HCC cell line HCCLM3 compared with MHCC97L cells. Furthermore, we evaluated uc.134 expression in clinical samples using in situ hybridization (ISH) and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The full-length transcript of uc.134 was confirmed using rapid amplification of cDNA ends (RACE) analyses. To investigate the biological function of uc.134, we performed gain-of-function and loss-of-function studies both in vitro and in vivo. The underlying mechanisms of uc.134 in HCC were investigated using RNA pulldown, RNA immunoprecipitation, ubiquitination assays, Western blotting, mRNA microarray analyses, and qRT-PCR analyses.

Results
The ISH assay revealed that uc.134 expression was significantly decreased in 170 paraffin-embedded samples from patients with HCC compared with adjacent tissues and uc.134 expression directly correlated with patient prognosis. Furthermore, we defined a 1867-bp full-length transcript of uc.134 using 5′- and 3′-RACE analysis. The overexpression of uc.134 inhibited HCC cell proliferation, invasion, and metastasis in vitro and in vivo, whereas the knockdown of uc.134 produced the opposite results. Furthermore, we confirmed that uc.134 (1408–1867 nt) binds to CUL4A (592–759 aa region) and inhibits its nuclear export. Moreover, we demonstrated that uc.134 inhibits the CUL4A-mediated ubiquitination of LATS1 and increases YAPS127 phosphorylation to silence the target genes of YAP. Finally, a positive correlation between uc.134, LATS1, and pYAPS127 was confirmed in 90 paraffin-embedded samples by ISH and immunohistochemical staining.

Conclusions
Our study identifies that a novel lncRNA, uc.134, represses hepatocellular carcinoma progression by inhibiting the CUL4A-mediated ubiquitination of LATS1 and increasing YAPS127 phosphorylation. The use of this lncRNA may offer a promising treatment approach by inhibiting YAP and activating Hippo kinase signaling.
BIM-Driven components library for Islamic Facilities (BIM-IF)
Authors: Almaimani Ayad K. , Nawari Nawari O. 
Published: April 2017

AbstractIslamic architectural history encompasses a substantial amount of information that needs to be categorized and digitally modeled so it can be utilized proficiently in the design phases of any project that seeks Islamic construction character. This paper centers on developing BIM-driven three-dimensional object library for Hejazi Islamic Architecture (HIAC) styles, construction methods, structural elements, and architectural components. The Ottoman style architecture has had profound effects on the Hijazi region for more than three hundred years. This influence remains largely uncategorized and digitally undocumented. Building Information Modelling (BIM) approach is used to develop parametric three-dimensional models of HIACs that are used in the structure and construction of Hijazi buildings. The HIACs are aggregated and classified according to their type and origin with respect to the chronological timeline of Islamic Architecture (IA). The creation of this BIM-Driven Components Library for Islamic Facilities (BIM-IF), thus allows for the use of HIACs in the design phases of projects that aim to incorporate IA styles. Each HIAC in the BIM-IA library contains architectural, structural and constructional data that is categorized using informatics tables appended with the digital models. In addition to the essential design data, the BIM-IF library provides designers with various historical information and details about numerous unique Ottoman styles.
Solving the tensorial 3D acoustic wave equation: A mimetic finite-difference time-domain approach
Authors: Shragge Jeffrey , Tapley Benjamin 
Published: April 2017

Generating accurate numerical solutions of the acoustic wave equation (AWE) is a key computational kernel for many seismic imaging and inversion problems. Although finite-difference time-domain (FDTD) approaches for generating full-wavefield solutions are well-developed for Cartesian computational domains, several challenges remain when applying FDTD approaches to scenarios arguably best described by more generalized geometry. In particular, how best to generate accurate and stable FDTD solutions for scenarios involving grids conforming to complex topography or internal surfaces. We address these issues by developing a mimetic FDTD (MFDTD) approach that combines four key components: a tensorial 3D AWE, mimetic finite-difference (MFD) operators, fully staggered grids (FSGs), and MFD Robin boundary conditions (RBC). The tensorial formulation of the 3D AWE permits wave propagation to be specified on (semi-) analytically defined coordinate meshes designed to conform to complex domain boundaries. MFD operators allow for higher order FD stencils to be applied throughout the model domain, including the boundary region where implementing centered FD stencils can be problematic. The FSG approach combines wavefield information propagated on four complementary subgrids to ensure the existence of all wavefield gradients required for computing the tensorial Laplacian operator, and thereby avoids interpolation approximations. The RBCs are implemented with a flux-preserving mimetic boundary operator that forestalls introduction of nonphysical energy into the grid by enforcing underlying flux-conservation laws. After validating the 3D MFDTD scheme on a sheared Cartesian mesh, we generate 3D wavefield simulation examples for internal boundary (IB) and topographic coordinate systems. The numerical examples demonstrate that the MFDTD scheme is capable of providing accurate and low-dispersion impulse responses for scenarios involving distorted IB meshes conforming to water-bottom surfaces and topographic coordinate systems exhibiting 2.5 km of topographic relief and including steep (65°) slope angles.
Two Components of Aversive Memory inDrosophila, Anesthesia-Sensitive and Anesthesia-Resistant Memory, Require Distinct Domains Within the Rgk1 Small GTPase
Authors: Murakami Satoshi , Minami-Ohtsubo Maki , Nakato Ryuichiro , Shirahige Katsuhiko , Tabata Tetsuya 
Published: April 2017

Multiple components have been identified that exhibit different stabilities for aversive olfactory memory inDrosophila. These components have been defined by behavioral and genetic studies and genes specifically required for a specific component have also been identified. Intermediate-term memory generated after single cycle conditioning is divided into anesthesia-sensitive memory (ASM) and anesthesia-resistant memory (ARM), with the latter being more stable. We determined that the ASM and ARM pathways converged on the Rgk1 small GTPase and that the N-terminal domain-deleted Rgk1 was sufficient for ASM formation, whereas the full-length form was required for ARM formation. Rgk1 is specifically accumulated at the synaptic site of the Kenyon cells (KCs), the intrinsic neurons of the mushroom bodies, which play a pivotal role in olfactory memory formation. A higher than normal Rgk1 level enhanced memory retention, which is consistent with the result that Rgk1 suppressed Rac-dependent memory decay; these findings suggest thatrgk1bolsters ASM via the suppression of forgetting. We propose that Rgk1 plays a pivotal role in the regulation of memory stabilization by serving as a molecular node that resides at KC synapses, where the ASM and ARM pathway may interact.SIGNIFICANCE STATEMENTMemory consists of multiple components.Drosophilaolfactory memory serves as a fundamental model with which to investigate the mechanisms that underlie memory formation and has provided genetic and molecular means to identify the components of memory, namely short-term, intermediate-term, and long-term memory, depending on how long the memory lasts. Intermediate memory is further divided into anesthesia-sensitive memory (ASM) and anesthesia-resistant memory (ARM), with the latter being more stable. We have identified a small GTPase inDrosophila, Rgk1, which plays a pivotal role in the regulation of olfactory memory stability. Rgk1 is required for both ASM and ARM. Moreover, N-terminal domain-deleted Rgk1 was sufficient for ASM formation, whereas the full-length form was required for ARM formation.
Abraham – ojciec wiary odważnej
Authors: Mazurek Mateusz 
Published: April 2017

Artykuł omawia i analizuje historię Abrahama i egzystencjalne implikacje, które z niej wypływają. W opinii autora historia ta ukazuje trzy szczególnie ważne aspekty: zbawienie jako wyzwolenie od lęku, głęboką wolność płynącą z takiego doświadczenia i posłuszeństwo Bogu jako przekroczenie tego, co rozumowo poznawalne. Wszystkie te (powiązane z sobą) aspekty życia chrześcijańskiego są niezwykle ważne. Dziś, kiedy kultura jest w przeważającej mierze antychrześcijańska, przekazuje i promuje anty­‑chrześcijańskie wzorce i wartości, jest szczególnie istotne, żeby wiara była wiarą odważną.
Pytanie o luterańską tożsamość
Authors: Medwid Wojciech 
Published: April 2017

Celem artykułu jest próba odpowiedzi na pytanie o luterańską tożsamość w dzisiejszym świecie na podstawie dokumentu Usprawiedliwienie i wolność, przygotowującego protestantów duchowo i organizacyjnie do uroczystości 500-lecia reformacji. Reformacja była wydarzeniem historycznym, które wpłynęło na kulturowe, społeczne i polityczne oblicze Europy. W centrum teologicznych wypowiedzi reformatorów znajduje się kwestia relacji Bóg–człowiek, a fundamentem ruchu z XVI wieku stało się usprawiedliwienie, które trzeba ujmować przez pryzmat miłości, uznania i docenienia, przebaczenia i wolności, aby zweryfikować wiarygodność doświadczenia iustificatio. Reformacja nie jest czymś zamkniętym, ale stanowi proces odnowy w duchu reformacyjnym. Teologia protestancka wyraża się w pięciu zasadach: solus Christus, sola gratia, solo verbo, sola scriptura i sola fide. Przez te elementy podjęto próbę przeniesienia idei usprawiedliwienia na obecny czas. Świętowanie 500-lecia reformacji ma się odbyć w rozsądnej równowadze między tradycją a innowacją, nie ignorując głębokiego znaczenia reformacji dla teraźniejszości. Dokument Usprawiedliwienie i wolność podejmujący temat usprawiedliwienia z łaski przez wiarę w Chrystusa poddano krytyce.
Arabscy chrześcijanie wobec astrologii na podstawie traktatu „O wierze chrześcijan w wyroki gwiazd” Eliasza z Nisibi
Authors: Sadowski Michał 
Published: April 2017

Eliasz z Nisibi należy do najbardziej płodnych pisarzy chrześcijańskich języka arabskiego swojej epoki. Jego siódmy maǧlis z Abī l­‑Qāsim dotyczy wyroków gwiazd i zasługuje na uwagę z powodu swej aktualności. Prezentowany przez Eliasza dyskurs można podzielić na dwie części: pierwszą mówiącą o wolności człowieka oraz drugą, w której wskazuje on na rolę ludzkiego rozumu. Te dwa elementy są głównymi argumentami w jego krytyce fatalizmu i astrologii. Jego nauczanie jest osadzone na wcześniejszym syryjskim chrześcijańskim dziedzictwie teologicznym. Jako logik niestrudzenie obnaża irracjonalny charakter wszelkich doktryn astrologicznych oraz fatalistych.
Making Sense of Advice About Drinking During Pregnancy: Does Evidence Even Matter?
Authors: Armstrong Elizabeth Mitchell 
Published: April 2017

ABSTRACTWomen have been told not to drink during pregnancy for decades; last year, the Centers for Disease Control and Prevention (CDC) extended that advice to all women who were at risk for experiencing a pregnancy. This commentary puts the recent CDC guidelines in historical perspective and considers the unintended consequences of public health messages that extend beyond what is supported by evidence.
Pengaruh Model Speciment Uji Tarik Pada Pengelasan Besi Fc-30 Di Lihat Dari Kekuatan Tarik Pengelasan
Authors: Mulyadi Mulyadi 
Published: April 2017

Uji Tarik adalah salah satu pengujian yang digunakan untuk mengetahui sifat mekanik logam maupun non logam. Uji tarik merupakan suatu metode yang digunakan untuk menguji kekuatan suatu bahan/material dengan cara memberikan beban gaya yang berlawanan arah. Pengujian tarik (tensile test) juga sering disebut sebagai tension test, merupakan salah satu dari pengujian mekanik yang paling mendasar/fundamental, sangat sederhana, tidak mahal dan telah distandarisasi di seluruh dunia seperti di Amerika ASTM E 8 dan ASTM E 8M dan di Jepang JIS 2241. Dapat mengetahui tegangan, regangan,reduksi, dan modulus elastisitas. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh model penampang speciment kotak dan bulat dengan luasan yang sama dilihat dari kekuatan tarik masing-masing specimen. Penelitian ini menggunakan metode eksperimen perbandingan, yaitu suatu cara untuk membandingkan antara dua indikator speciment yang berbeda model penampang dengan luasan yang sama, untuk mengetahui kekuatan tarik masing-masing speciment . Hasil penelitian menunjukkan bahwa kekuatan tarik pada material dasar sebesar 781,004Mpa dengan speciment bentuk kotak. Kekuatan tarik paling tinggi terjadi pada pengelasan menggunakan elektroda las LB-52 yaitu sebesar 1.193,033 Mpa penampang speciment kotak dan elektroda las CIA yang memiliki kekuatan tarik yang hampir sama yaitu sebesar 1.134,002 Mpa penampang speciment kotak. Sedangkan yang paling rendah adalah pengelasan menggunakan elektroda LB-52 yang tanpa dicelup oli dimana hampir sama dengan material dasarnya yaitu sebesar 403,802 Mpa penampang speciment bulat.
Aplicación de la ingeniería kansei con redes neuronales
Authors: Avendaño Prieto Gerardo , Álvarez L. Héctor René 
Published: April 2017

ONTARE. REVISTA DE INVESTIGACIÓN DE LA FACULTAD DE INGENIERÍA
La Ingeniería Kansei, relaciona las emociones que sienten los consumidores con las características y propiedades que poseen los productos a través de la estimación de un modelo matemático, que permite establecer cuáles tienen mayor relación con las emociones, permitiendo al diseñador, incorporarlas con el fin de activar los factores que las intensifican y dar soluciones efectivas de diseño.
Tradicionalmente, la formulación y estimación del modelo matemático, se hace través de un modelo de regresión múltiple (QT1) y análisis factorial; sin embargo, una de las desventajas es que está condicionado a cumplir los supuestos del modelo. En este trabajo, se muestra cómo las redes neuronales se pueden aplicar en los estudios de Ingeniería Kansei y nos da resultados similares, permitiendo que se puedan utilizar cuando no se cumplen los supuestos estadísticos.
Understanding Seal Sections and the Phantom Failures
Authors: Merrill Dan , Dwiggins Jeff 
Published: April 2017

Abstract
Seal/protector sections can be difficult to understand and properly apply. The components in seal/protector sections can fail for reasons that are not fully understood or obvious. Typically, the driving factor for seal/protector section application is temperature (thermal) cycles. Some advocate the use of more mechanical shaft seals, while others advocate parallel chamber configurations, allowing for more expansion/contraction. The question is, "What is the correct approach to take for seal/protector selection?" This paper will attempt to address some of these issues, and focus on seal/protector section failures and areas for improvement. This paper will also give an overall explanation of how seal/protector sections should work and the problems that are often encountered. A current practice in the industry is to apply tandem seal/protector sections as a method of redundancy and in theory, increasing reliability. Advanced materials developed for the harsh temperature environments may be helpful in adapting a single seal section capable of providing superior motor protection in comparison to tandem seals.
Ultra-High Speed ESP PMM System Application in Salym Petroleum Development
Authors: Gorlov A. , Shakirov A. 
Published: April 2017

Abstract
Salym Petroleum Development (SPD) is a 50:50 joint venture between Shell and GazPromNeft. SPD has been developing the Salym group of fields in Western Siberia since 2003, having now over 600 ESP lifted wells. Salym group of fields are characterized by harsh conditions with scale & solids being the major cause of ESP failures. Since the average Water Cut exceeded 80%, SPD was carefully reviewing available technologies to economically produce low rate wells (&lt;1000 bblpd) while assuring target drawdowns and with the ability to be installed below perforations passing high DLS sections.
One of the studied technologies was the Ultra-High Speed ESP System, which is utilizing a Permanent Magnet Motor with rotational speeds between 1,000 and 12,000 rpm. Due to its high nameplate operating speed of 10,000 rpm, the system is significantly shorter in length (2-3 times less compared against a conventional ESP length). Permanent magnet motors provide high power efficiency (over 90%), and high abrasion resistance (2 g/l) is achieved with hard alloys. Innovative modular pump design insures maximum efficiency of the system that could not be introduced to the industry in the past. It should be noted that conventrional ESPs have a narrow operating speed range of conventional ESPs (2400-4200 rpm).
This paper will provide a summary of the use of Ultra-High Speed ESP PMM System and refer to a project with recent field applications as good examples of its use. The proposed technology has been proven to be a great success at Salym Group of Fields and indications are that it has good potential to replace conventional ESPs in both low flow rate and high flow rate applications.
Design, Operation, Diagnosis, Failure Analysis and Optimization of ESP Systems in Wells with Great Depths, High Temperature, High GOR and High Concentrations of CO2, N2, H2S in Samaria Luna Field
Authors: Ramirez M. , Martinez J. F. 
Published: April 2017

Abstract
The following study considers the ESP system implementation experience from the year 2009 to 2016 in the challenging field of Samaria Luna located in the Southern Region of Mexico. During this time frame, many well failures occurred in the different processes of the ESP system. The study will present a comprehensive methodology that provides solutions to ultimately extend the run life of wells with ESP system. It will also discuss the solutions provided to the Samaria Luna project aiming to achieve an acceptable run life (MTBF) and compare it with international well run life indicators operating under similar field conditions of temperature, depth, gas, and other adverse conditions for artificial lift systems.
The diagnosis, failure analysis and root cause analysis for the time frame evaluation played an important role in the construction of the methodology. The results of the evaluation oriented to correction making, decision making and appropriate optimization strategies in various technical aspects such as: the different stages of the ESP system, energy quality, equipment maintenance, pump design, pump installation, well operation and well stabilization. Another important element that will be discussed in the methodology is the external close supervision from PEMEX to manufacturers and vendors in the field, the office and software use and application in the different stages of the project.
There are currently not many fields in the world with these characteristics. However, over time artificial lift systems such as gas lift become inefficient due to an increase in the water cut parameter. For this reason, in more fields of the world the eventual decision is to transition to ESP as the alternative artificial lift system due to the advantages of operating in greater depth reservoirs and having much higher volumes of production.
It is important to share the lessons learnt: optimization strategies, design methodologies, positive and negative operational practices and other technical actions that have been executed in the Samaria Luna project that may assist as reference to other fields in the world under similar operating conditions (high temperature above 280 F, great depths above 12,000 Ft, high GOR above 1500 SCF/STB).
The recommendations of this project may be applied in other projects and directly or indirectly help improve the run life of wells with ESP system, increasing the project profitability in any part of the world.
A DESMATERIALIZAÇÃO DO REAL EMPRICO E SUA TRANSFIGURAÇÃO EM ESTADO DE ALMA EM MADONA DOS PRAMOS
Authors: Gomes Carmelita Rodrigues 
Published: April 2017

O presente artigo tem como pretensão fazer uma breve análise da transfiguração do real no romance Madona dos Páramos (1982) do escritor mato-grossense Ricardo Guilherme Dicke. Observa-se, no presente romance, que os elementos da realidade exterior local, que atestam o romance como autêntico regionalista, aos poucos, passam por um processo de polimento. Primeiro, são reproduzidos de acordo com a realidade empírica, posteriormente, são enfeitados, retocadas, ampliado e, por último, atingem a condição de metáfora passam por um processo de porosidade – são transfigurados. O objetivo do artigo, portanto, é desvendar, no tecido labiríntico e retorcido da narrativa, qual é o sentido dessas transfigurações. Primeiro, procurarei demonstrar que tais representações têm a ver com interioridade psicológica dos homens configurados no romance. Segundo, verificar em que aspecto a forma como foi configurado o assunto no romance foi um recurso projetado pelo escritor para materializar ou para reforçar a condição de queda dos personagens que ele efetivou ao colocá-los em cena.
O gênero biografia e a paisagem cultural: construções do patrimônio cultural
Authors: Garcia Boldorini Marília , Barros Meira Roberta 
Published: April 2017

Este texto pretende verificar a importância da literatura, por meio dogênero textual biografia, para a construção de representações do patrimônio cultural de determinado grupo social com foco na paisagem cultural. A literatura consiste num conjunto de obras literárias de reconhecido valor estético de um país, por vezes retratando o povo, os lugares, as tradições e os costumes.Para o desenvolvimento do trabalho, procurou-se ressaltar quão significante é a paisagem cultural em textos da literatura. Afinal de contas, a paisagem cultural na literatura consiste numa representação conforme a perspectiva do autor. A investigação, de cunho descritivo e qualitativo, terá a linguagem como o ponto primordial da abordagem, com base em fontes bibliográficas e documentais. Portanto, intenciona-se com isso perceber se é possível considerar textos literários em estudos sobre a paisagem cultural, além de constatar se a literatura pode ser vista como auxílio na construção do patrimônio cultural.
Da sustentabilidade ou insustentabilidade da natureza do patrimônio natural
Authors: Arruda Gilmar 
Published: April 2017

Nas últimas décadas do século XX ocorreu a “inflação do patrimônio” e seexpandiu a noção de sustentabilidade, surgindo o patrimônio natural como uma das respostas à percepção de que a natureza estaria em risco por conta das atividades humanas. Neste artigo tentamos responder, com a ajuda da história ambiental, três indagações decorrentes desses eventos, ou dessas ideias: 1) O que sustentaria a natureza do patrimônio?; 2) O patrimônio natural seria sustentável?; 3) A naturezado patrimônio natural seria sustentável?