bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rab11 collaborates E‐cadherin to promote collective cell migration and indicates a poor prognosis in colorectal carcinoma
Authors: Chung Yuan‐Chiang , Wei Wan‐Chen , Hung Chia‐Nung , Kuo Jen‐Fang , Hsu Chih‐Ping , Chang King‐Jen , Chao Wei‐Ting 
Published: October 2016

AbstractBackgroundCollective cell migration, whereby the cell–cell contacts such as E‐cadherin are maintained during migration, has only recently emerged, and its detailed mechanisms are still unclear. In this study, the role of Rab11, which functions in recycling endosomes, and its relationship to E‐cadherin in colorectal carcinoma were identified, and the role of Rab11 in the collective cell migration of colon cancer cells was clarified.Materials and methodsA total of 107 patients with surgically resected colorectal carcinoma were enrolled in this immunohistochemical study. Relationships between the overexpression of Rab11 and E‐cadherin and survival were evaluated. The cell biology of Rab11 overexpression or knock‐down in HT‐29 colon cells was studied.ResultsThe expression of Rab11 and E‐cadherin was not correlated with the stage of cancer or lymph node metastasis. However, the overall survival was poor in the group of 67 patients with duo‐positive Rab11 and E‐cadherin expression compared to the group (40 patients) without dual‐positive expression (P = 0·038). Rab11 was demonstrated to have a physical interaction with E‐cadherin, and overexpression of Rab11 was found to promote collective cell migration through the increased distribution of E‐cadherin, which enhanced cell–cell connections. In addition, Rac1 activation and matrix metalloproteinase‐2 expressions were upregulated upon Rab11 expression.ConclusionsThis study demonstrated that Rab11 and E‐cadherin expressions are indicators of poor survival time in colorectal carcinoma, but that Rab11 overexpression may contribute to increased collective cell invasion in colorectal carcinoma.
The anthelmintic effects of five plant extracts on the viability of Parascaris equorum larvae
Authors: Rakhshandehroo E. , Asadpour M. , Malekpour S. H. , Jafari A. 
Published: October 2016

SummaryAscariasis is a very common parasitic disease in equids, especially in young horses. Despite the use of anthelmintic drugs, resistance has been frequently reported in populations of Parascaris equorum. As a result, herbal preparations are proposed for current control strategies. In this study, a modified method was used for hatching the eggs of P. equorum. After hatching, the effects of methanolic extract of Artemisia dracunculus, Eucalyptus camadulensis, Mentha pulegium, Zataria multiflora and Allium sativum (garlic) were investigated on the recovered larvae. For each extract, the anthelmintic effects of different concentrations (50, 75, 100 and 125 mg/mL) were evaluated at 0, 10, 20, 30 and 40 min after the challenge. The results showed that our modifications to the older method could enhance the hatching rate for the eggs of P. equorum (to an average of 98%). Potassium dichromate was also demonstrated in this study to be a favourable medium during embryonation. In addition, all the concentrations of A. dracunculus and M. pulegium and higher levels (≥100 mg/mL concentrations) of Z. multiflora extracts had significant lethal effects on larvae from the first to the fourth 10 min of the experiment. In contrast, E. camadulensis and A. sativum had not marked effects on larvae viability at any time of the challenge. In conclusion, our data suggest that A. dracunculus, M. pulegium and Z. multiflora have potential to be used as anthelmintic for the control of ascariasis in equid host; however, these effects remain to be confirmed through in vivo studies.
Environmental DNA analysis for estimating the abundance and biomass of stream fish
Authors: Doi Hideyuki , Inui Ryutei , Akamatsu Yoshihisa , Kanno Kazuki , Yamanaka Hiroki , Takahara Teruhiko , Minamoto Toshifumi 
Published: October 2016

Summary

Environmental DNA (eDNA) analysis for detecting the presence of aquatic and terrestrial organisms is an established method, and the eDNA concentration of a species can reflect its abundance/biomass at a site. However, attempts to estimate the abundance/biomass of aquatic species using eDNA concentrations in large stream and river ecosystems have received little attention.

We determined the eDNA concentration and abundance/biomass of a stream fish, Plecoglossus altivelis, by conducting a snorkelling survey in the Saba River, Japan. Furthermore, we evaluated the relationship between eDNA concentrations and the estimated abundance/biomass of P. altivelis, and determined its spatial distribution within the river.

Across the three seasons from spring to autumn, we found significant correlations between the eDNA concentration of P. altivelis and its abundance/biomass at study sites within the river. We detected the eDNA at the sites where we found only feeding traces on stones (where P. altivelis was not directly observed), but not at sites without feeding traces. Additionally, we tested the optimal number of qPCR replicates needed for the eDNA evaluation of P. altivelis abundance and biomass; only a small number of replicates was required when the eDNA concentration was high.

Our findings suggest that eDNA analysis is a useful tool to estimate fish abundance/biomass as well as their spatial distribution in rivers.
Diet and Headache: Part 2
Authors: Martin Vincent T. , Vij Brinder 
Published: October 2016

BackgroundComprehensive diets do not require the exclusion of a specific provocative food or ingredient, but regulate the quantities of core components of foods such as vitamins, ions, proteins, carbohydrates, and fats.ObjectivesTo review the evidence supporting the use of comprehensive diets in the prevention of migraine and other headache disorders and to discuss the mechanisms through which food, and ingredients within foods and beverages might trigger attacks of headacheMethodsThis represents Part 2 of a narrative review of the role of diet in the prevention of migraine and other headache disorders. A PubMed search was performed with the following search terms: “folate,” “vitamin D,” “low fat diet,” “omega‐3 and omega‐6 fatty acid diet,” “ketogenic diet,” “Atkins diet,” and “sodium.” Each of these search terms was then crossreferenced with “headache” and “migraine” to identify relevant studies. Only studies that were written in English were included in this review.ResultsLow fat and high omega‐3/low omega‐6 fatty diets decrease the frequency of attacks of migraine and/or other headache disorders as demonstrated in two separate randomized controlled trials. A ketogenic diet was more effective than a standard diet in reducing the frequency of migraine in a single nonrandomized clinical study. An observation study found that dietary consumption of folate was inversely associated with the frequency of migraine attacks in persons with migraine with aura that have the C variant of the methylene tetrahydrofolate reductase gene. The mechanisms though which diets may precipitate headache include their effects on neuropeptides, neuro‐receptors and ion channels, inflammation, sympathetic nervous system, release of nitric oxide, vasodilation, and cerebral glucose metabolism.ConclusionsEvidence exists to support the use of comprehensive diets in the prevention of migraine and other headache disorders. However, the results of these studies should be considered preliminary until replicated in larger randomized controlled clinical trials.
Assessment of quality of life, anxiety, socio‐economic factors and caries experience in Brazilian children with overweight and obesity
Authors: Araujo DS , Marquezin MCS , Barbosa TS , Fonseca FLA , Fegadolli C , Castelo PM 
Published: October 2016

AbstractPurpose: This study evaluated the association between excess weight and quality of life (QoL), symptoms of anxiety, caries experience and socio‐economic factors in a representative sample of 8‐ to 10‐year‐old children from three public schools of Piracicaba (SP, Brazil). Materials and methods: The Autoquestionnaire Qualité de Vie Enfant Image was applied to explore family and social relations, activities, health, body functions and separation domains, and, by means of the Multidimensional Anxiety Scale for Children, symptoms of anxiety were screened. Clinical examination was performed using DMFT/dmft indexes to assess oral health and caries experience. Medical and nutritional history, parents’ schooling, monthly income and ownership of household goods and services were also evaluated. Results: Of the 313 subjects included, four subjects were underweight, 188 normal weight, 67 overweight and 54 presented obesity (38.7% with excess weight). Measures of QoL, anxiety scores and caries experience did not differ between groups. The regression model showed a significant association between excess weight and the ownership of household goods and services (OR = 5.4/CI = 1.6–18.3). Conclusions: High prevalence of excess weight was observed among prepubertal children, emphasizing the need for continued health programmes to limit risk factors for obesity. QoL, anxiety scores and caries experience did not differ between subjects with different body weights, although children from public schools with higher ownership of goods and services were more likely to present excess weight.
Laparoendoscopic single‐site surgeries: A multicenter experience of 469 cases in Japan
Authors: Sato Fuminori , Nakagawa Ken , Kawauchi Akihiro , Matsubara Akio , Okegawa Takatsugu , Habuchi Tomonori , Yoshimura Koji , Hoshi Akio , Kinoshita Hidefumi , Miyajima Akira , Naitoh Yasuyuki , Inoue Shogo , Itaya Naoshi , Narita Shintaro , Hanai Kazuya , Okubo Kazutoshi , Yanishi Masaaki , Matsuda Tadashi , Terachi Toshiro , Mimata Hiromitsu 
Published: October 2016

ObjectiveTo report on a multi‐institutional series of non‐robotic urological laparoendoscopic single‐site surgery in Japan.MethodsConsecutive cases of laparoendoscopic single‐site surgery carried out between February 2009 and December 2012 at nine academic institutions were included. We examined the surgical outcomes, including conversion and complications rates.ResultsFour hundred and sixty‐nine cases were included in the analysis. The most common procedure was adrenalectomy (n = 177) and the second most common procedure was radical nephrectomy (n = 143). The procedures also included nephroureterectomy (n = 40), living donor nephrectomy (n = 40), pyeloplasty (n = 30), urachal remnant excision (n = 9), simple nephrectomy (n = 7), radical prostatectomy (n = 6) and others (n = 17). The access sites included umbilicus (n = 248, 53%) and other sites (n = 221, 47%). A transperitoneal approach was used in 385 cases (82%), and retroperitoneal approach in 84 cases (18%). The median operation time of all procedures was 198 min. Conversion to reduced port surgery, conventional laparoscopy, or open surgery was noted in 27 cases (5.8%), 12 cases (2.6%), and two cases (0.4%), respectively, with an overall conversion rate of 8.7%. Intraoperative complications occurred in 10 cases (2.1%). Post‐operative complications were noted in 29 cases (6.2%), including five major complications (1.1%). No mortality was recorded in this series.ConclusionsNon‐robotic laparoendoscopic single‐site surgery is technically feasible and safe for various urologic diseases in Japan. Furthermore, urological laparoendoscopic single‐site surgery is a promising minimally invasive surgical option that is feasible for experienced urological surgeons in intermediate‐volume centers as well as high‐volume centers.
Too scared to go sick? The management and the manifestations of workplace attendance in the food retail sector
Authors: Hadjisolomou Anastasios 
Published: October 2016

AbstractThis article is a response to Taylor et al’s (2010) call for further research regarding workplace attendance. It examines the new politics of absence management in the UK and Cyprus in food retail sector, identifying a dual approach in managing attendance across the two countries. The first approach suggested the penalization of absence, whilst the second focused on the prevention of absence through accommodation. The article argues that ‘coercion’ is not the sole vehicle to tackle high absence in food retailing rather it suggests the development of other practices to enhance cooperation to regular attendance. The research identifies significant differences regarding the formality of these processes between the two countries, and suggests the generation of a particular attendance culture within the organisations, one that is not merely a culture of fear, as Taylor et al (2010) suggest, but rather a culture that includes accommodation and cooperation.
The long noncoding RNA‐ROR promotes the resistance of radiotherapy for human colorectal cancer cells by targeting the p53/miR‐145 pathway
Authors: Yang Pengxiang , Yang Yue , An Weiwei , Xu Jianyu , Zhang Gan , Jie Jing , Zhang Qingyuan 
Published: October 2016

AbstractBackground and AimLong intergenic noncoding RNAs (lincRNAs) have critical roles in elevating efficacy of anticancer therapy and tumor progression. Recent studies show that Regulator of Reprogramming (ROR) is aberrantly expressed in several types of cancer, including colorectal cancer (CRC). Radiotherapy is considered as a standard preoperative treatment. However, a considerable number of CRCs are resistant to radiotherapy. In this study, we evaluated the role of lincRNA‐ROR in radiotherapy for CRC and detected the underlying molecular mechanism.MethodsReal‐time polymerase chain reaction was employed to quantify the expression level of lincRNA‐ROR in different CRC cell lines and tissue samples. Cell viability and apoptosis assays were used to confirm the radiotherapy‐mediated effects by lincRNA‐ROR altered expression. The direct impact of lincRNA‐ROR on the expression of p53/miR‐145 by loss‐of‐function and gain‐of‐function strategy was also analyzed. A xenograft mouse model was used to evaluate the role of linc‐ROR in CRC treatment.ResultsWe discovered that lincRNA‐ROR was upregulated in CRC cell lines and tissue samples. We further showed that knockdown of lincRNA‐ROR enhanced the sensitivity to radiotherapy for CRC by inhibiting cell viability and promoting apoptosis. Activity of the p53/miR‐145 pathway may help explain the role of lincRNA‐ROR for stress‐induced regulation in CRC therapy. Combined specific knockdown of lincRNA‐ROR and radiotherapy treatment in xenograft model resulted in a significant reduction in the tumor growth.ConclusionLincRNA‐ROR decreases sensitivity to radiotherapy via the negative regulation of p53/miR‐145 and may represent a potential target for the treatment of CRC.
Delirium After Spine Surgery in Older Adults: Incidence, Risk Factors, and Outcomes
Authors: Brown Charles H. , LaFlam Andrew , Max Laura , Wyrobek Julie , Neufeld Karin J. , Kebaish Khaled M. , Cohen David B. , Walston Jeremy D. , Hogue Charles W. , Riley Lee H. 
Published: October 2016

ObjectivesTo characterize the incidence, risk factors, and consequences of delirium in older adults undergoing spine surgery.DesignProspective observational study.SettingAcademic medical center.ParticipantsIndividuals aged 70 and older undergoing spine surgery (N = 89).MeasurementsPostoperative delirium and delirium severity were assessed using validated methods, including the Confusion Assessment Method (CAM), CAM for the Intensive Care Unit, Delirium Rating Scale‐Revised‐98, and chart review. Hospital‐based outcomes were obtained from the medical record and hospital charges from data reported to the state.ResultsThirty‐six participants (40.5%) developed delirium after spine surgery, with 17 (47.2%) having purely hypoactive features. Independent predictors of delirium were lower baseline cognition, higher average baseline pain, more intravenous fluid administered, and baseline antidepressant medication. In adjusted models, the development of delirium was independently associated with higher quintile of length of stay (odds ratio (OR) = 3.66, 95% confidence interval (CI) = 1.48–9.04, P = .005), higher quintile of hospital charges (OR = 3.49, 95% CI = 1.35–9.00, P = .01), and lower odds of discharge to home (OR = 0.22, 95% CI = 0.07–0.69, P = .009). Severity of delirium was associated with higher quintile of hospital charges and lower odds of discharge to home.ConclusionDelirium is common after spine surgery in older adults, and baseline pain is an independent risk factor. Delirium is associated with longer stay, higher charges, and lower odds of discharge to home. Thus, prevention of delirium after spine surgery may be an important quality improvement goal.
Diagnostic accuracy of des‐gamma‐carboxy prothrombin for hepatocellular carcinoma in a French cohort using the Lumipulse® G600 analyzer
Authors: Sultanik P. , Ginguay A. , Vandame J. , Popovici T. , Meritet J.‐F. , Cynober L. , Pol S. , Bories P.‐N. 
Published: October 2016

SummaryThe increasing incidence of hepatocellular carcinoma (HCC) in Western countries requests reliable tumour markers for preclinical diagnosis. We evaluated the diagnostic accuracy of des‐gamma‐carboxy prothrombin (DCP), in comparison with alpha‐fetoprotein (AFP) in a French cohort using a new analyser. One hundred and sixty‐two patients with virus‐related cirrhosis (46 HCC patients and 116 controls) were recruited in this retrospective proof‐of‐concept study. DCP was measured on new Lumipulse® G600 analyzer and AFP on usual Cobas e602 analyzer in serum samples that were collected at the time of HCC diagnosis for HCC patients or during follow‐up for controls. DCP and AFP levels were higher in HCC patients. The area under receiver operating characteristic curve was larger for DCP than for AFP (0.89 vs 0.77, P=.03). At the cut‐off value of 128 mAU/mL, sensitivity and specificity for DCP were 74% and 92%. At the cut‐off value of 20 μg/L, sensitivity and specificity for AFP were 63% and 82%. NRI>0 for the association of “AFP+DCP” were 101%, P<.0001, and 23%, P=.03, compared to “AFP” or “DCP” alone, respectively. We conclude that DCP outperformed AFP for the detection of HCC.
Effects of Varying Levels of High‐Protein Distiller's Grains on Growth Performance of Channel Catfish, Ictalurus punctatus, and Post‐Extrusion Feed Pellet Characteristics
Authors: Tidwell J. H. , Coyle S. D. , Bright L. A. , Pires L. , Rosentrater K. A. 
Published: October 2016

AbstractChannel catfish, Ictalurus punctatus, remains the USA's largest aquaculture industry. However, in recent years, high feed prices have negatively impacted profitability. Production of ethanol as a biofuel has increased dramatically. Companies have modified their processes to enhance ethanol yield and these modified processes also increase the crude protein content of the byproduct. The object of this study was to evaluate this high‐protein dried distiller's grains (HP‐DDG) for its suitability in catfish diets. A total of 25 full‐sibling channel catfish (7.1 ± 2.3 g) were randomly stocked into each of twelve 260‐L polyethylene tanks to achieve 25 fish per tank. The control diet was similar to a commercial formulation. Experimental diets contained 20, 40, or 40% HP‐DDG with added lysine (1% of total diet). There were three replicate tanks per diet. Fish were fed to apparent satiation twice daily for 9 wk. Average harvest weights for fish fed the 40% HP‐DDG diet without lysine supplementation (57.0 g) were significantly smaller than that for fish fed the control diet (77.7 g). Average harvest weights of fish fed 20% HP‐DDG (86.8 g) were significantly greater than that of fish fed either 40% HP‐DDG (57.0 g) or 40% HP‐DDG + lysine (73.7 g). There were no significant differences in feed conversion ratios or survivals among treatments, which overall averaged 1.1 and 99%, respectively. These data indicate that HP‐DDG appears to be a suitable ingredient in channel catfish diets up to at least 20% of the total formulation. Data also indicate that inclusion levels of at least 40% HP‐DDG are likely limiting in lysine.
Intravenous iron and erythropoiesis‐stimulating agents in haemodialysis: A systematic review and meta‐analysis
Authors: Roger Simon D , Tio Martin , Park Hyeong‐Cheon , Choong Hui Lin , Goh Bakleong , Cushway Timothy Robert , Stevens Vanessa , Macdougall Iain C 
Published: October 2016

ABSTRACTAimHigher dosages of erythropoiesis‐stimulating agents (ESAs) have been associated with adverse effects. Intravenous iron is used to optimize ESA response and reduces ESA doses in haemodialysis patients; this meta‐analysis evaluates the magnitude of this effect.MethodsA literature search was performed using MEDLINE, Embase and the Cochrane Collaboration Central Register of Clinical Trials from inception until December 2014, to identify randomized controlled trials of intravenous iron and ESA, in patients undergoing haemodialysis for end‐stage kidney disease. Dosing of IV iron in concordance with the Kidney Disease Improving Global Outcomes guidelines was considered optimal iron therapy.ResultsOf the 28 randomized controlled trials identified, seven met the criteria for inclusion in the meta‐analysis. Results of random‐effects meta‐analysis show a statistically significant weighted mean (95% CI) difference of −1733 [−3073, −392] units/week in ESA dose for optimal iron versus suboptimal iron. The weighted average change in ESA dose was a reduction of 23% (range −7% to −55%) attributable to appropriate dosing of intravenous iron. A comparison of intravenous iron versus oral iron/no iron (five trials) showed a greater reduction in ESA dose, although this did not reach statistical significance (weighted mean difference, 95% CI: −2,433 [−5183, 318] units/week). The weighted average change in ESA dose across the five trials was a reduction of 31% (range −8% to −55%).ConclusionSignificant reductions in ESA dosing may be achieved with optimal intravenous iron usage in the haemodialysis population, and suboptimal iron use may require higher ESA dosing to manage anaemia.
Angioedema After Squaric Acid Treatment in a 6‐Year‐Old Girl
Authors: Chen Chen (Amy) , Carlberg Valerie , Kroshinsky Daniela 
Published: October 2016

AbstractAlopecia areata (AA) involves the immune‐related destruction of hair follicles, resulting in patches of complete hair loss, most often on the scalp. The topical sensitizer squaric acid dibutylester (SADBE) is a popular treatment option given its low side‐effect profile, hair regrowth potential, and lack of cross‐reactivity with other chemicals. We describe a unique case of a 6‐year‐old girl who developed angioedema after SADBE treatment for AA.
Lymphocyte‐depleting induction therapy lowers the risk of acute rejection in African American pediatric kidney transplant recipients
Authors: Crowson Cole N. , Reed Rhiannon D. , Shelton Brittany A. , MacLennan Paul A. , Locke Jayme E. 
Published: October 2016

AbstractThe use of lymphocyte‐depleting induction immunosuppression has been associated with a reduction in risk of AR after KT among adult recipients, particularly among high‐risk subgroups such as AAs. However, data on induction regimen and AR risk are lacking among pediatric KT recipients. We examined outcomes among 7884 first‐time pediatric KT recipients using SRTR data (2000‐2014). Characteristics were compared across race using Wilcoxon rank‐sum tests for continuous and chi‐square tests for categorical variables. Risk of AR was estimated using modified Poisson regression, stratified by recipient race, adjusting for recipient age, gender, BMI, primary diagnosis, number of HLA mismatches, maintenance immunosuppression, and donor type. Risk of AR within 1 year was lower in AA recipients receiving lymphocyte‐depleting induction (ATG or alemtuzumab; RR, 0.66; 95% CI, 0.52‐0.83 P < .001) compared to AA recipients receiving anti‐IL‐2 receptor antibody induction. This difference was not seen in non‐AA recipients receiving lymphocyte‐depleting induction (RR, 0.93; 95% CI, 0.81‐1.06, P = .26) compared to IL‐2 induction. These findings support a role for lymphocyte‐depleting induction agents in AA pediatric patients undergoing KT and continued use of IL‐2 inhibitor induction in non‐AA pediatric KT recipients.