bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional Diversification of the Four MARCKS Family Members in Zebrafish Neural Development
Authors: Prieto Daniel , Zolessi Flavio R. 
Published: August 2016

ABSTRACTMyristoylated alanin‐rich C‐kinase substrate (MARCKS) and MARCKS‐like 1, each encoded by a different gene, comprise a very small family of actin‐modulating proteins with essential roles in mammalian neural development. We show here that four genes (two marcks and two marcksl1) are present in teleosts including zebrafish, while ancient actinopterigians, sarcopterigian fishes, and chondrichtyans only have two. No marcks genes were found in agnaths or invertebrates. All four zebrafish genes are expressed during development, and we show here how their early knockdown causes defects in neural development, with some phenotypical differences. Knockdown of marcksa generated embryos with smaller brain and eyes, while marcksb caused different morphogenetic defects, such as larger hindbrain ventricle and folded retina. marcksl1a and marcksl1b morpholinos also caused smaller eyes and brain, although marcksl1a alone generated larger brain ventricles. At 24 hpf, marcksb caused a wider angle of the hindbrain walls, while marcksl1a showed a “T‐shaped” neural tube and alterations in neuroepithelium organization. The double knockdown surprisingly produced new features, which included an increased neuroepithelial disorganization and partial neural tube duplications evident at 48 hpf, suggesting defects in convergent extension. This disorganization was also evident in the retina, although retinal ganglion cells were still able to differentiate. marcksl1b morphants presented a unique retinal phenotype characterized by the occurrence of sporadic ectopic neuronal differentiation. Although only marcksl1a morphant had a clear “ciliary phenotype,” all presented significantly shorter cilia. Altogether, our data show that all marcks genes have functions in zebrafish neural development, with some differences that suggest the onset of protein diversification.
Analysis of the articular cartilage T1ρ and T2 relaxation times changes after ACL reconstruction in injured and contralateral knees and relationships with bone shape
Authors: Pedoia Valentina , Su Favian , Amano Keiko , Li Qi , McCulloch Charles E. , Souza Richard B. , Link Thomas M. , Ma Benjamin C. , Li Xiaojuan 
Published: August 2016

ABSTRACTThe objectives of this study were twofold: (1) to evaluate the longitudinal change in cartilage T1ρ and T2 6‐ and 12‐months after ACL reconstruction (ACLR) in both reconstructed and intact contralateral knees with the aim of validating the role of the contralateral knee as an internal control in longitudinal studies; (2) to explore relationships between bone shape at the time of injury and the progression of T1ρ and T2 over 12‐months after ACLR. T1ρ and T2 cartilage relaxation times and 3D MRI‐based statistical shape modeling (SSM) of tibia and femur were computed for both knees of forty ACL‐injured patients and 15 healthy controls. ACL subjects were scanned 8.4 ± 6.4 weeks after injury (2.4 ± 3.7 weeks prior to ACLR), 6‐ and 12‐months after ACLR. Longitudinal changes in T1ρ and T2 values were assessed using linear mixed model, and partial correlation coefficients were calculated between bone shape and longitudinal changes in T1ρ and T2 values. Significant longitudinal increases in T1ρ and T2 values were observed in reconstructed and contralateral knees 6‐months after ACLR. Tibial bone shape features, associated with the medial plateau height and width, were observed to be correlated with cartilage T1ρ and T2 progression in reconstructed knees. Our results suggest that caution should be used in considering contralateral knee as internal controls in longitudinal ACL studies and 3D MRI‐based‐SSM might serve as an imaging biomarker for the early stratification of patients at risk for developing post‐traumatic accelerated cartilage degeneration and potentially osteoarthritis after ACL tear. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:707–717, 2017.
Non‐destructive prediction of pigment content in lettuce based on visible–NIR spectroscopy
Authors: Steidle Neto Antonio José , Moura Lorena de Oliveira , Lopes Daniela de Carvalho , Carlos Lanamar de Almeida , Martins Luma Moreira , Ferraz Leila de Castro Louback 
Published: August 2016

AbstractBACKGROUNDLettuce (Lactuca sativa L.) is one of the most important salad vegetables in the world, with a number of head shapes, leaf types and colors. The lettuce pigments play important physiological functions, such as photosynthetic processes and light stress defense, but they also benefit human health because of their antioxidant action and anticarcinogenic properties. In this study three lettuce cultivars were grown under different farming systems, and partial least squares models were built to predict the leaf chlorophyll, carotenoid and anthocyanin content.RESULTSThe three proposed models resulted in high coefficients of determination and variable importance for the projection values, as well as low estimative errors for calibration and external validation datasets. These results confirmed that it is possible to accurately predict chlorophyll, carotenoid and anthocyanin content of green and red lettuces, grown in different farming systems, based on the spectral reflectance from 500 to 1000 nm.CONCLUSIONThe proposed models were adequate for estimating lettuce pigments in a quick and non‐destructive way, representing an alternative to conventional measurement methods. Prediction accuracies were improved by using the detrending, smoothing and first derivative pretreatments to the original spectral signatures prior to estimating lettuce chlorophyll, carotenoid and anthocyanin content, respectively. © 2016 Society of Chemical Industry
Effects of ZnO nanoparticle‐coated packaging film on pork meat quality during cold storage
Authors: Suo Biao , Li Huarong , Wang Yuexia , Li Zhen , Pan Zhili , Ai Zhilu 
Published: August 2016

AbstractBACKGROUNDThere has been limited research on the use of ZnO nanoparticle‐coated film for the quality preservation of pork meat under low temperature. In the present study, ZnO nanoparticles were mixed with sodium carboxymethyl cellulose (CMC‐Na) to form a nanocomposite film, to investigate the effect of ZnO nanoparticle‐coated film on pork meat quality and the growth of bacteria during storage under low temperature.RESULTSWhen ZnO nanoparticle‐coated film was used as the packaging material for pork meat for 14 days of cold storage at 4 °C, the results demonstrated a significant effect on restricting the increases in total volatile basic nitrogen and pH levels, limiting the decreases of lightness (increased L* value) and redness (increased a* value), and maintaining the water‐holding capacity compared to the control pork samples (P < 0.05). The present study also discovered that the ZnO nanoparticle‐coated film restrained the increase in total plate count (TPC). When Staphylococcus aureus was used as the representative strain, scanning electron microscopy revealed that ZnO nanoparticles increased the occurrence of cell membrane rupture under cold conditions.CONCLUSIONZnO nanoparticle‐coated film helps retain the quality of pork meat during cold storage by increasing the occurrence of microorganism injury. © 2016 Society of Chemical Industry
Reconstructive transoral laser microsurgery for posterior glottic web with stenosis
Authors: Atallah Ihab , Manjunath M. Krishniah , Omari Ahmad Al , Righini Christian Adrien , Castellanos Paul F. 
Published: August 2016

Objectives/HypothesisTo demonstrate that reconstructive transoral laser microsurgical (R‐TLM) techniques can be used for the treatment of symptomatic laryngeal posterior glottic web–based stenosis (PGWS) in a large cohort of patients utilizing a postcricoid mucosal advancement flap (PCMAF).Study DesignRetrospective cohort review.MethodsA consecutive series of patients with PGWS who underwent R‐TLM using a PCMAF were reviewed for outcomes. After laser excision of the PGWS scar and mobilization of fixed cricoarytenoid joints, a PCMAF was raised using microinstruments and a scanning free‐beam CO2 laser. The flap was advanced and attached over the scar bed using a technique with multiple novel features that make it easy to adopt.ResultsFifty‐two patients were treated. Of the cases, 42.3% had a tracheostomy at presentation with grade II to IV PGWS, and 46% of cases had grade III to IV PGWS. In all cases, R‐TLM was the only treatment approach. No open reconstructions were performed. No airway stents were used. Patients without tracheostomy, regardless of the grade of stenosis, did not require a tracheostomy to undergo this operation. All tracheostomy patients were successfully decannulated. All patients without a tracheostomy had significant improvement of their respiratory symptoms on the Dyspnea Index (mean Δ = 14.75, P value <.01).ConclusionsRTLM using the PCMAF is a feasible, safe, and effective alternative to open approaches for airway reconstruction for PGWS. This novel transoral technique includes a much simpler endoscopic suturing alternative to knot tying among other new features. It is reproducible and reliable for laryngologists familiar with laryngeal microsurgery.Level of Evidence4. Laryngoscope, 127:685–690, 2017
The utility of genetic testing in neuromuscular disease: A consensus statement from the AANEM on the clinical utility of genetic testing in diagnosis of neuromuscular disease
Authors: Kassardjian Charles D. , Amato Anthony A. , Boon Andrea J. , Childers Martin K. , Klein Christopher J. ,   AANEM Professional Practice Committee
Published: August 2016

ABSTRACTIntroduction: The aim of this consensus statement is to provide a recommendation from AANEM experts on the clinical utility of genetic testing. It is not meant to recommend or endorse any specific genetic testing methodology or algorithm. Methods: The AANEM Professional Practice Committee reached a consensus based on expert opinion on the utility of genetic testing in neuromuscular diseases and made recommendations on factors that physicians and patients should consider when deciding whether to proceed with such testing. Results: Despite the costs of genetic testing, these tests can be both valuable and beneficial in the diagnosis and treatment of neuromuscular diseases in many situations. Conclusions: The AANEM believes that performing genetic testing to arrive at a specific molecular diagnosis is a critical step in providing high‐quality care to neuromuscular patients. The cost of testing should not be a deterrent, as there are important clinical, safety, psychosocial, and research benefits. Muscle Nerve 54: 1007–1009, 2016
Pediatric malignant germ cell tumors: A comparison of the neuro‐oncology and solid tumor experience
Authors: Plant Ashley S. , Chi Susan N. , Frazier Lindsay 
Published: August 2016

AbstractMalignant germ cell tumors (GCT) arise from abnormal migration of primordial germ cells and are histologically identical whether they occur inside or outside the central nervous system (CNS). However, the treatment strategy for GCTs varies greatly depending on the location of the tumor. These differences are in part due to the increased morbidity of surgery in the CNS but may also reflect differential sensitivity of the tumors to chemotherapy and radiation therapy (RT) or not‐yet‐understood biologic differences between these tumors. Historically, specialists caring for extracranial and intracranial GCT in the United States have practiced separately without much cross communication. The focus of this review is a discussion of differences between the management of CNS and extra‐CNS GCTs and opportunities for collaboration and future research.
Asparaginase‐associated pancreatitis is not predicted by hypertriglyceridemia or pancreatic enzyme levels in children with acute lymphoblastic leukemia
Authors: Raja Raheel Altaf , Schmiegelow Kjeld , Sørensen Ditte Nørbo , Frandsen Thomas Leth 
Published: August 2016

AbstractBackgroundl‐Asparaginase is an important drug for treatment of childhood acute lymphoblastic leukemia (ALL), but is associated with serious toxicities, including pancreatitis and hypertriglyceridemia (HTG). Asparaginase‐associated pancreatitis (AAP) is a common reason for stopping asparaginase treatment. The aim of this study was to explore if HTG or early elevations in pancreatic enzymes were associated with the subsequent development of AAP.MethodChildren (1.0–17.9 years) diagnosed with ALL, treated with asparaginase for 30 weeks, according to the NOPHO ALL2008 protocol at the University Hospital Rigshospitalet, Copenhagen, Denmark, were eligible. Pancreatic enzymes, triglycerides, and cholesterol were measured regularly.ResultsThirty‐one patients were included. Seven patients were diagnosed with AAP. HTG was most evident when PEG‐asparaginase and dexamethasone were administered concomitantly. Overall, there was no significant difference in triglyceride levels in patients who experienced AAP and patients who did not. An increase in triglyceride levels during concomitant dexamethasone therapy in delayed intensification was significantly associated with an increase in pancreas‐specific amylase levels two weeks later (P = 0.005).ConclusionsAAP does not seem to be associated with HTG. Continuous monitoring of pancreas enzymes does not predict AAP.
High‐Temperature Structural Transformations of 1,1‐Diamino‐2,2‐dinitroethene (FOX‐7)
Authors: Zakharov Viktor V. , Chukanov Nikita V. , Dremova Nadezdha N. , Chervonnyi Alexandr D. , Shilov Gennadiy V. , Korsounskii Boris L. , Kazakov Anatoliy I. , Volkova Nina N. 
Published: August 2016

AbstractStuctural transformations of 1,1‐diamino‐2,2‐dinitroethene (FOX‐7) were investigated in the temperature range 298–513 K by means of DSC, TG, isothermal calorimetry, PXRD, IR spectroscopy, and electron microscopy. The data obtained confirm the existence of the high‐temperature δ‐FOX‐7 polymorph stable above 480 K. The heat effect of the γ→δ transformation is − 4.6 J g−1 (−680 J mol−1). Metastable γ‐phase formed in the reverse process δ→γ has a perfect crystal structure and is stable towards thermal decomposition. Possible mechanisms of sharp deceleration of thermal decomposition of FOX‐7 at the 40 % conversion are discussed.
Electrical transport properties of a superconductor–ferrimagnet composite in applied magnetic fields
Authors: Kalanda Nikolai A. , Yarmolich Marta V. , Sobolev Nikolai A. 
Published: August 2016

Electrical transport properties of the YBa2Cu3O7−δ + Sr2FeMoO6−δ superconductor–ferrimagnet composite as a function of the temperature and applied magnetic field are reported. Four different intervals in the temperature dependence of the resistivity have been found between room temperature and 4.2 K. In the temperature range from 300 K down to a semiconducting behavior is observed, whereas below the first transition into a superconducting state sets in. Upon further cooling down, the semiconducting behavior appears again below and then a second superconducting transition starts at continuing down to 4.2 K. The magnetoresistance is negative above and positive below it, achieving at 5 K and a field of 10 T a huge value of ∼5700%. We discuss the role of the weak links between the YBa2Cu3O7−δ grains represented by the Sr2FeMoO6−δ interlayers on the magnetotransport in the composite.Temperature dependence of the electrical resistivity of the YBCO + 0.05 SFMO composite measured in different applied magnetic fields, where the temperatures refer to zero field, and .
Nontargeted analysis of the urine nonpolar sulfateome: a pathway to the nonpolar xenobiotic exposome
Authors: Yao Yuanyuan , Wang Poguang , Shao Gang , Del Toro Liza V. Anzalota , Codero Jose , Giese Roger W. 
Published: August 2016

RationaleTesting the urine nonpolar sulfateome can enable discovery of xenobiotics that are most likely to be bioactive. This is based on the fact that nonpolar xenobiotics are more likely to enter cells where they tend to undergo metabolism, in part, to sulfates that are then largely excreted into the urine.MethodsThe following sequence of steps, with conditions that achieve high reproducibility, was applied to large human urine samples: (1) competitive nonpolar extraction with a porous extraction paddle; (2) weak anion‐exchange extraction with strong organic washing; and (3) ultrahigh‐performance liquid chromatography (UHPLC)/negative ion matrix‐assisted laser desorption ionization tandem time‐of‐flight mass spectrometery (MALDI‐TOF/TOF‐MS) with recording of ions with signal‐to‐noise (S/N) ≥ 20 that yielded M–1–80 (loss of SO3) or m/z 97 (HSO4−) upon fragmentation.ResultsFrom a collection of urine samples from six pregnant women, the masses of 1129 putative sulfates were measured. Three lists of candidate compounds (preliminary hits) from these masses were formed by searching METLIN, especially via MATLAB, yielding putative xenobiotic contaminants (35 compounds), steroids (122), and flavonoids (1582).ConclusionsA new way to reveal some of the nonpolar xenobiotic exposome has been developed that applies to urine samples. The value of the method is to suggest xenobiotics for subsequent targeted analysis in the population of people under study, in order to relate the environment to health and disease. Copyright © 2016 John Wiley & Sons, Ltd.
Water Quality Changes Shortly After Low‐Head Dam Removal Examined With Cultural and Microbial Source Tracking Methods
Authors: Bohrerova Z. , Park E. , Halloran K. , Lee J. 
Published: August 2016

AbstractShort‐term effects of low head dam removal on water quality of urbanized stream were evaluated, focusing on fecal pollution indicators. Composite river samples were analyzed for Escherichia coli concentrations, nitrates, phosphates, turbidity and human‐specific marker (HF183) and antibiotic resistance marker (tetQ) before and after dam removal during dry weather conditions. The sampled Olentangy River water in summer before the dam removal showed poor water quality with mean E. coli concentration of 439 colony forming unit (CFU)/100 mL, mean turbidity of 10 NTU and mean nutrient concentrations of 0.61 and 0.41 mg/L for nitrate and phosphate, respectively. Surprisingly, even one month after the dam removal, E. coli numbers doubled and nitrate concentration tripled compared to pre‐removal concentrations. Although the detected HF183 concentrations were below the quantifiable levels, they did not correlate with E. coli concentrations, suggesting E. coli from other than human fecal origin. The correlation between turbidity and E. coli during dry weather further suggests E. coli accumulation in impoundment sediments and release once dam was removed. These short‐term effects of dam removal on water quality should be further evaluated, especially if recreation and other beneficial uses of water in the area are expected. Copyright © 2016 John Wiley & Sons, Ltd.
Thermal reductive disproportionation of 3,3′,5,5′‐tetraphenyldiphenoquinone with drastic color change: Potential prototype of data storage advanced materials
Authors: Hossain Md. Awlad , Akiyama Kazuhiko , Goto Kenta , Sugiura Ken‐ichi 
Published: August 2016

AbstractThe solid‐state thermal reaction of brilliant red 3,3′,5,5′‐tetraphenyldiphenoquinone 1 was examined. Three reductive colorless products, the corresponding hydroquinone and two benzofuran derivatives, were formed at 289.5 °C. Theoretical studies indicated that thermally excited triplet species having radical‐type oxygen atoms participated in the reaction. Single‐crystal diffraction study revealed short atomic contacts between carbonyl oxygen and the ortho‐positions of the phenyl group, and these short atomic contacts would promote the thermal solid‐state reaction. The reductive reactions observed in cumene, a radical propagation solvent and/or a hydrogen radical donating solvent, supported our speculation.
The Study of the Interaction between Doxorubicin and Single‐Stranded DNA
Authors: Chen Xing , Zhou Lu , Wang Jine , Jiang Guimei , Cheng Hui , Pei Renjun 
Published: August 2016

AbstractThe binding properties of doxorubicin with single stranded DNA of various base compositions were determined by fluorescence and circular dischroism assays. The interaction of Dox with ssDNA comprising of single repeating deoxynucleotide was firstly investigated. Dox preferentially bound to the G residues among four bases. Then oligonucleotides covering potential GA, GT, GC or GG binding sites were designed to quantitatively analyze the preferential base composition at the binding site. Dox exhibited better effect on oligonucleotide with GC sites. To testify the binding preference to GC site, GC‐rich oligonucleotides containing two GC fragments with different array modes were employed. Dox was verified to preferentially bind to the GC site of single stranded DNA. The binding of Dox with GC site in single stranded DNA may involve in the cooperation interaction of multiple GC with one Dox molecule. It appears that Dox may prefer stacking interaction with the multiple GC nucleotide bases along the oligonucleotide backbone.
Revisiting the corporate social performance‐financial performance link: A replication of Waddock and Graves
Authors: Zhao Xiaoping , Murrell Audrey J. 
Published: August 2016

Research summary: In this study, we revisit the relationship between corporate social performance (CSP) and corporate financial performance (CFP) by conducting a replication of Waddock and Graves (1997). Using 1990 KLD ratings as the CSP measure, the original study reports a positive bidirectional relationship between CSP and CFP. However, our replication analyses with a larger sample over a longer time period indicate that the findings of the original study may not be generalizable to different samples. We argue that our replication casts doubt on the original study and can serve as a starting point to reconsider the CSP‐CFP relationship. Based on the findings of our replication, we discuss the differences between the replication results and the original findings, and then suggest several approaches to revise and extend the original study.Managerial summary: Advocates of corporate social performance (CSP) have long argued that “doing good leads to doing well.” However, the evidence to support this argument is not strongly convincing, and managers hence doubt whether better CSP leads to improved corporate financial performance (CFP). In this article, we directly examine the relationship between CSP and CFP. Our article reports that CSP may not have a positive influence on CFP. Instead, our article shows the complexity of the relationship between CSP and CFP. Therefore, we cannot simply argue that doing good will necessarily lead to doing well. Copyright © 2016 John Wiley & Sons, Ltd.