bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accumulation of Cd and Pb in soils irrigated with sewage and non-sewage water in Prayagraj, India
Authors: VERMA JEETENDRA , MANI DINESH , SAHU VIPIN , SHUKLA DEVI PRASAD , VISHWAKARMA HIMANCHAL , KUMAR MANOJ , OJHA RUPESH KUMAR 
Published: September 2024

Unmanaged agricultural fields and the extended usage of sewage irrigation water can raise the concentration of heavy metals (Cd and Pb) in the soil, turning it into barren soil. The study was conducted during 2022-23 at four sewage and non-sewage irrigated sites in Prayagraj namely Naini, Baxibandh, Rasulabad and Draupadi Ghat. The representative soil samples were taken at depths of 0-15, 15-30, 30-45 and 45-60 cm. Di-acid mixture method was used for the estimation of heavy metals (Cd and Pb) in the soil sample by AAS (Atomic Absorption Spectrophotometer) (AAnalyst600, Perkin Elmer Inc., MA, USA). Contamination factor (CF) and enrichment factor % (EF) of different sites showed various results which fulfill the basic criteria of present and future prospective of research. The highest concentration of Cd and Pb was found in the surface layer (0-15 cm) of sewage and non-sewage irrigated sites at Naini i.e., Cd (4.46±0.42 and 1.05±0.08 mg/kg) and Pb (6.53±0.62 and 1.18±0.12 mg/kg) respectively. The lowest concentration of Cd and Pb was found in the sub-surface layer (45-60 cm) of sewage and non-sewage irrigated sites at Draupadi Ghat i.e., Cd (1.02±0.09 and 0.16±0.01 mg/kg) and Pb (1.68±0.12 and 0.18±0.01 mg/kg) respectively. The concentration of heavy metals cadmium (Cd) and lead (Pb) declined with soil profile depth in both sewage and non-sewage irrigated soils. The long-term persistence of heavy metal contamination in soil can lead to severe issues; therefore, regular monitoring of sewage-irrigated soils is necessary to minimize heavy metal pollution.
Enhancing cadmium phytoremediation in contaminated soil using sunflower (Helianthus annuus L.) with EDTA and vermicompost amendments
Authors: KUMAR MANOJ , MANI DINESH , SAHU VIPIN , VERMA JEETENDRA , OJHA RUPESH KUMAR , PATEL SURAJ 
Published: September 2024

The study hypothesis posits that EDTA, an effective chelating agent, can effectively mobilize cadmium from soil, augmenting the sunflower's natural ability to absorb and sequester heavy metals. Concurrently, vermicompost, rich in organic matter and beneficial microorganisms, enhances soil health and supports plant growth, thereby fortifying the sunflower's phytoremediative potential. The pot experiment was carried out as an overall completely randomized design (CRD) at the Sheila Dhar Institute (SDI) experimental farm, University of Allahabad, Prayagraj, in the Kharif season during the years 2023-2024. Plants were cultivated in pots with soil (5 kg) that contained varying concentrations of Cd (0, 15, 30, and 50 mg/kg), vermicompost (0 and 10 g/kg), and EDTA (0 and 2.5 mmol/kg). Plants were also cultivated in containers with clean soil (T1) as control treatment. For analyzed heavy metal (Cd) in plants with a tri-acid mixture (15 ml) containing concentrations of HNO3, H2SO4, and HClO4 in 5:1:2 ratios, use the Atomic Absorption Spectrophotometer (AAS). The result showed that the combined Treatment (T3) pot of application of Cd 0+ EDTA (2.5 mmol/kg) and vermicompost (10 g/kg) was the maximum dry biomass (14.43 g/pot) of the sunflower plant. While the maximum dose combined treatment (T12) application of Cd 50 mg/kg+ EDTA 2.5 mmol/kg and vermicompost 10 g/kg is greater than before, the maximum accumulation of Cd in the shoot and root of Helianthus annuus L. by shoot 37.16% and root 31.16%, respectively as compared to the control treatment pot Cd-50. The results indicate that the combined application of EDTA and vermicompost significantly increased cadmium uptake by sunflowers compared to untreated controls, demonstrating their synergistic effect in improving phytoremediation efficiency.
Analisis Efisiensi Boiler Menggunakan Metode Direct dan Indirect pada PLTU Jeneponto 2×135 MW
Authors: Habibuddin Musdholifah , Anshar Muhammad , Firman Firman 
Published: September 2024

Salah satu mesin industri yang perannya sangat penting yaitu boiler. Boiler dalam perusahaan ini merupakan asset yang sangat penting bagi perusahaan, apabila terjadi masalah pada sistem boiler, maka kelancaran steam (uap) akan terganggu sehingga efisiensi boiler akan mengalami penurunan, oleh karena itu sangat diperlukan untuk mengkaji ulang kinerja mesin agar tidak terjadi pemborosan energi. Objek utama pada penelitian ini ialah Boiler PLTU Jeneponto 2 × 135 MW. Metode yang digunakan untuk menentukan efisiensi boiler yaitu metode langsung (Direct Method) dan metode tak langsung (Indirect Method). metode langsung (Direct Method) yaitu energi yang didapat dari fluida kerja (air dan steam) dibandingkan dengan energi yang terkandung dalam bahan bakar boiler, sedangkan metode tak langsung (Indirect Method) yaitu efisiensi merupakan perbedaan antara kehilangan panas (Heat loss) dan energi yang masuk. Penelitian ini bertujuan untuk mengetahui seberapa besar penurunan efisiensi boiler. Berdasarkan hasil penelitian dengan metode langsung (Direct Method) efisiensi boiler di tahun 2021 dan 2022 yaitu 42,63 % dan 64,11 % sedangkan dengan metode tak langsung (Indirect Method) efisiensi boiler sebelum dan sesudah overhaul yaitu 80,09 % dan 80,71 %. Nilai kehilangan panas (Heat Losses) karena gas buang yang kering (Ldg) sebelum dan sesudah overhaul sebesar 6,40 % dan 5,87 %. Kehilangan panas karena penguapan air yang terbentuk oleh adanya H2 dalam bahan bakar (LH) sebesar 6,25 % dan 6,21 %. Kehilangan panas karena penguapan kadar air dalam bahan bakar (Lmf) sebesar 5,50 % dan 5,48 %. Kehilangan panas karena kadar air dalam udara (Lma) memiliki nilai yang sama yaitu 0,26 % , dan kehilangan panas karena radiasi dan kehilangan lain yang tak terhitung (Luc) sebesar 1,5 % .
تأثير برنامج تعليمي بأسلوب الأنظمة التمثيلية في تعلم مهارة قفزة اليدين الأمامية على بساط الحركات الارضية بالجمناستك الفني للطالبات
Authors: Doha Imad Majeed  , Asst. Prof. Dr. Abeer Ali Hussein  
Published: September 2024

يهدف البحث الى اعداد برنامج برنامج تعليمي بأسلوب الأنظمة التمثيلية في تعلم مهارة قفزة اليدين على بساط الحركات الارضية بالجمناستك الفني لدى طالبات المرحلة الثالثة في كلية التربية البدنية والعلوم الرياضية – جامعة كربلاء ، تم التعرف على تأثير برنامج تعليمي بالأسلوب الانظمة التمثيلية في تعلم مهارة قفزة اليدين على بساط الحركات الارضية ، فضلا عن التعرف على افضلية التأثير في تعلم مهارة قفزة اليدين الامامية على بساط الحركات الارضية بالجمناستك بين البرنامج التعليمي بأسلوب الانظمة التمثيلية والمنهج المعتمد من المدرس. وقد استخدمت الباحثتان المنهج التجريبي بتصميم المجموعتين المتكافئة (الضابطة والتجريبية) ذات الاختبار القبلي والبعدي وهو ما تراه ينسجم ويتطابق مع مواصفات مشكلة بحثها وتحقيق أهداف دراستها،تحدد مجتمع البحث بطالبات المرحلة الثالثة في كلية التربية البدنية وعلوم الرياضة جامعة كربلاء للعام الدراسي (2023 – 2024) والبالغ عددهم (59) طالبة مقسمين على اربعة شعب (A ، B، C ، D) واختيرت عينة استطلاعية بأسلوب المعاينة العشوائية البسيطة بواقع (10) طالبة تشكلت ما نسبته (16.9%) من عناصر المجتمع واختيار العينة الرئيسية للبحث بأسلوب المعاينة العشوائية البسيطة بواقع (30) طالبة تشكلت ما نسبته (50.8%) من المجتمع اذ مثل (15) طالبة من شعبة (B,A) المجموعة التجريبية ومثل (15) طالبة من شعبة (C,D) المجموعة الضابطة.
تأثير الية التعلم التعاوني للتعلم النشط في تعلم بعض المسكات بالمصارعة الحرة للطلاب
Authors: Asst. Prof. Dr. Jalal Abdul Zahra Kanaan  
Published: September 2024

تتجلى أهمية البحث لرفع التعليم وبصورة نشطة وتعاونية لأداء أنواع المسكات بالمصارعة للطلاب وبذلك ننجح في اخراج درس ملائم للتعلم وفعال والارتقاء بمستوى الطلاب لتعلم تلك المسكات وتتطلب بعض الالعاب الرياضية صعبة الممارسة الى مستوى تعليمي مناسب واسلوب تدريسي يعمل على الارتقاء ببعض المهارات والحركات الصعبة مثل لعبة المصارعة التي تعتمد على الاداء لبعض المسكات ذات الصعوبة وتحتاج الى تعليم نشط يرفع من مستوى التعلم الصحيح. ويهدف البحث الى التعرف على تأثير الية التعلم التعاوني للتعلم النشط في تعلم بعض المسكات بالمصارعة الحرة للطلاب. واستنتج الباحث ان الية التعلم التعاوني للتعلم النشط مهمة ولها اسلوب تدريسي ناجح في تعلم بعض المسكات بالمصارعة الحرة للطلاب. ويوصي الباحث باعتماد الية التعلم التعاوني للتعلم النشط لأنها مهمة ولها اسلوب تدريسي ناجح في تعلم بعض المسكات بالمصارعة الحرة للطلاب.
Rapid genome-wide profiling of DNA methylation and genetic variation using guide positioning sequencing (GPS)
Authors: Zhang Baolong , Li Wei , Li Jin , Li Yan , Luo Huaibing , Xi Yanping , Dong Shihua , Wu Feizhen , Yu Wenqiang 
Published: September 2024

Whole-genome bisulfite sequencing (WGBS) has been extensively utilized for DNA methylation profiling over the past decade. However, it has shown limitations in terms of high costs and inefficiencies. The productivity and accuracy of DNA methylation detection rely critically on the optimization of methodologies and the continuous refinements of related sequencing platforms. Here, we describe a detailed protocol of guide positioning sequencing (GPS), a bisulfite-based, location-specific sequencing technology designed for comprehensive DNA methylation characterization across the genome. The fundamental principle of GPS lies in the substitution of dCTP with 5-methyl-dCTP (5 mC) at the 3′-end of DNA fragments by T4 DNA polymerase, which protects cytosines from bisulfite conversion to preserve the integrity of the base composition. This alteration allows the 3′-end to independently facilitate genetic variation profiling and guides the 5′-end, enriched with methylation information, to align more rapidly to the reference genome. Hence, GPS enables the concurrent detection of both genetic and epigenetic variations. Additionally, we provide an accessible description of the data processing, specifically involving certain software and scripts. Overall, the entire GPS procedure can be completed within a maximum of 15 days, starting with a low initial DNA input of 100–500 ng, followed by 4–5 days for library construction, 8–10 days for high-throughput sequencing (HTS) and data analysis, which can greatly facilitate the promotion and application of DNA methylation detection, especially for the rapid clinical diagnosis of diverse disease pathologies associated with concurrent genetic and epigenetic variations.
Metabolomics-based biomarkers of fermented dairy and red meat intake: a randomized controlled trial in healthy adults
Authors: La Barbera Giorgia , Praticò Giulia , Dragsted Lars Ove , Cuparencu Catalina 
Published: September 2024

BackgroundDietary assessment is usually performed through imprecise tools, leading to error-prone associations between diet and health-related outcomes. Metabolomics has been applied in recent years to develop biomarkers of food intake (BFIs) and to study metabolites in the diet-microbiome crosstalk. Candidate BFIs exist to detect intake of meat and to a lesser extent dairy, but validation and further development of BFIs are needed. Here, we aim to identify biomarkers that differentiate between intakes of red meat and dairy, to validate previously reported BFIs for these foods, and to explore the effect of protein-matched meals on selected microbial metabolites.MethodsWe conducted a randomized, controlled, cross-over single-meal study comparing a meal with highly fermented yogurt and cheese, and a meal with beef and pork meatballs. Postprandial urine samples from 17 subjects were collected sequentially after each meal up to 24 h and analyzed by untargeted metabolomics through ultra-high-performance-liquid chromatography (UHPLC) coupled via electrospray (ESI) source to a qTOF mass spectrometer. Univariate (repeated measures ANOVA) and multivariate (PLSDA, ML-PLSDA) data analyses were used to select BFIs differentiating the two meals. 3-Indoxyl sulfate, p-cresol sulfate, and several other microbial amino acid catabolites were additionally explored within the urine profiles.ResultsThirty-eight markers of meat and dairy intake were selected and are presented along with their excretion kinetics. Carnosine, taurine, and creatine, as well as hydroxyproline-based dipeptides are confirmed as meat BFIs. For dairy, previously reported metabolites such as acyl-glycines are confirmed, while proline-based dipeptides are reported as novel putative BFIs. Microbial metabolites showed only marginal evidence of differential formation after the two meals.ConclusionThis study allowed us to validate the postprandial kinetics of previously suggested biomarkers of meat and dairy intake and to identify new potential biomarkers. The excretion kinetics are useful to ensure that the collection of urine covers the correct time window in future dietary studies. The BFIs add to the existing body of biomarkers and may further be used in combination to provide a more reliable assessment of meat and dairy intake. Proteolytic microbial metabolites should be further investigated to assess the effect of different protein sources on health.
Special needs teachers in emergency remote teaching during the COVID-19 pandemic: the role of personal resources and teaching motivation on burnout
Authors: De Stasio Simona , Ragni Benedetta , Boldrini Francesca , Paoletti Daniela , Marchetti Vanessa , Rappazzo Maria Cristina , Berenguer Carmen 
Published: September 2024

BackgroundThis cross-sectional study explores the connections between burnout, personal resources, and teaching motivation among Italian special needs teachers (SNTs) one year following the start of the COVID-19 pandemic. It specifically seeks to determine if and how personal resources and teaching motivation can help SNTs by buffering the effects of burnout symptoms.MethodsThe study was conducted using a sample of 837 full-time, in-service kindergarten and primary teachers at public schools in Italy. Data were collected through self-report questionnaires, including the Copenhagen Burnout Inventory, the Santa Clara Brief Compassion Scale, the Crèche Educators Emotional Style Questionnaire, and the Autonomous Motivations for Teaching Scale. A multiple regression model was performed to assess the predictive role of personal resources and teaching motivation on the burnout of special needs teachers.ResultsThe findings indicate that higher levels of autonomous motivation are associated with reduced burnout, specifically among special education teachers. Conversely, higher levels of negative emotion regulation strategies were linked to increased burnout, and this relationship was stronger in mainstream teachers than in special education teachers. We found no significant association between teachers’ compassion and burnout.ConclusionsOur research supports the need for educational policymakers and school leaders to focus on personal resources and work-related well-being to enhance and support teachers, in particular during emergencies (e.g., the COVID-19 pandemic) and for specific groups of teachers, such as SNTs.
Association between female infertility and stroke mortality: evidence from the PLCO cancer screening trial
Authors: Tang Hui , Yang Xueming , Li Zhou , Zhang Yuan , Chen Huaxuan , Dai Mingjun , Shao Chuan 
Published: September 2024

ObjectiveWhile infertility affects about 15% of women during their reproductive years, its long-term impact on stroke mortality after this period remains unclear. This study aims to investigate the association between infertility and stroke mortality in women using data from the Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial.MethodsWe analyzed data from 75,778 female participants aged 55–74 years with a median follow-up of 16.84 years. Cox proportional hazard models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for stroke mortality, adjusting for potential confounders.ResultsAmong participants, 14.53% reported infertility. During follow-up, 1,159 women died from stroke. Compared to women without infertility, those with infertility had a higher risk of stroke mortality (HR 1.21, 95% CI 1.04–1.41, p = 0.016). This association remained statistically significant after adjusting for age, race, education level, marital status, smoking status, body mass index, history of hypertension, history of heart attack, history of diabetes mellitus, birth control pill use, hormone replacement therapy, endometriosis, first menstrual period and pregnancy history (HR 1.20, 95% CI 1.02–1.42, p = 0.029). Sensitivity and subgroup analyses yielded consistent results.ConclusionThe findings of this study indicate that infertility is associated with an increased risk of stroke mortality in women. Further research is needed to confirm these findings and elucidate the underlying mechanisms.
User expectations for an automated motorway access assistant system
Authors: Ehrhardt Sofie , Martin Manuel , Hild Jutta , Deml Barbara 
Published: September 2024

IntroductionTo avoid the uncertainty present in a cooperation between manual vehicles (MVs) and automated vehicles (AVs), cooperative connected automated mobility (CCAM) is frequently proposed. In this contribution, we consider the situation of a motorway slip road where an AV (SAE level 3 or higher) on the motorway cooperates by opening a suitable gap for an entering AV. To ensure a meeting of the cooperation partners, speed adjustment by the system might often be necessary.MethodsTo investigate the acceptance of an automated cooperative motorway access assistant a driving simulator study (N = 41) was conducted. Participants drove six laps on a virtual test track in a level 3 AV. Three levels of speed adjustment and two levels of an HMI (with or without HMI) were tested. They experienced the automated cooperative motorway access assistant in two situations. Firstly, when they entered the motorway themselves and benefited from the system, and secondly, when the system reduced their vehicle's speed to allow for cooperation with another vehicle on a motorway slip road ahead.ResultsThe results show that the acceptance of the assistant depends significantly on the magnitude of speed reduction: the greater the speed adjustment, the worse the user rating, and the more often the cooperation was terminated. Obviously, the acceptance depends on the perspective: If the users themselves benefit from the speed adjustment of others, they rate the system significantly better than if they themselves must accept the restriction. Only in the case of a small speed reduction, the assistant is rated as well as if the user benefits from the system. The availability of a human-machine interface (HMI) providing information about the system status influenced acceptance only marginally but had a significant influence on the trust in the automation.DiscussionThe results suggest that people are willing to accept a personal disadvantage in order to give others an advantage in the context of CCAM. However, acceptance depends on the magnitude of the disadvantage. The overall agreement scores of the system with HMI did not differ from those without HMI, although the HMI was found to slightly reduce the termination of cooperation. Three possible reasons for this result, which differs from previous research, are discussed.
In-utero transfer of decidualized endometrial stromal cells increases the frequency of regulatory T cells and normalizes the abortion rate in the CBA/J × DBA/2 abortion model
Authors: Zarnani Kayhan , Zarnani Kimia , Maslehat-Lay Nasim , Zeynali Bahman , Vafaei Sedigheh , Shokri Mohammad-Reza , Vanaki Negar , Soltanghoraee Haleh , Mirzadegan Ebrahim , Edalatkhah Haleh , Naderi Mohammad-Mehdi , Sarvari Ali , Attari Farnoosh , Jeddi-Tehrani Mahmood , Zarnani Amir-Hassan 
Published: September 2024

IntroductionFailure to adequate decidualization leads to adverse pregnancy outcomes including pregnancy loss. Although there are plenty of reports underscoring immune dysfunction as the main cause of abortion in CBA/J females mated with DBA/2 males (CBA/J × DBA/2), little is known about the potential role of impaired endometrial decidualization.MethodsEndometrial stromal cells (ESCs) from CBA/J mice were in-vitro decidualized, and the proteome profile of the secretome was investigated by membrane-based array. CBA/J mice were perfused In-utero with either decidualized ESCs (C×D/D), undecidualized ESCs (C×D/ND), or PBS (C×D/P) 12 days before mating with DBA/2 males. Control mice were not manipulated and were mated with male DBA/2 (C×D) or Balb/c (C×B) mice. On day 13.5 of pregnancy, reproductive parameters were measured. In-vivo tracking of EdU-labeled ESCs was performed using fluorescence microscopy. The frequency of regulatory T cells (Tregs) in paraaortic/renal and inguinal lymph nodes was measured by flow cytometry. The proliferation of pregnant CBA/J splenocytes in response to stimulation with DBA/2 splenocytes was assessed by 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) flow cytometry.ResultsIn C×D/D mice, the resorption rate was reduced to match that seen in the C×B group. Intrauterine perfused ESCs appeared in uterine stroma after 2 days, which remained there for at least 12 days. There was no difference in the number of implantation sites and embryo weight across all groups. The frequency of Tregs in the inguinal lymph nodes was similar across all groups, but it increased in the paraaortic/renal lymph nodes of C×D/D mice to the level found in C×B mice. No significant changes were observed in the proliferation of splenocytes from pregnant C×D/D compared to those of the C×D group in response to stimulation with DBA/2 splenocytes. Decidualization of ESCs was associated with a profound alteration in ESC secretome exemplified by alteration in proteins involved in extracellular matrix (ECM) remodeling, response to inflammation, senescence, and immune cell trafficking.DiscussionOur results showed that the deficiency of Tregs is not the primary driver of abortion in the CBA/J × DBA/2 model and provided evidence that impaired endometrial decidualization probably triggers endometrial immune dysfunction and abortion in this model.
Opportunistic infections changed before and after SARS-CoV-2 infection in inflammatory bowel disease patients: a retrospective single-center study in China
Authors: Fan Zhenzhen , Zhou He , Zhang Jiaqi , Liu Xiaoning , Wu Tong , Shi Yanting , Lin Junchao , Liang Jie 
Published: September 2024

ObjectiveSARS-CoV-2 transmission has become a serious worldwide public health concern. However, there is currently insufficient data to determine whether SARS-CoV-2 infection would affect opportunistic infections in inflammatory bowel disease (IBD) patients.MethodsA retrospective study included 451 IBD patients (294 UC and 157 CD). The IBD patients were divided into two groups: before SARS-CoV-2 infection and after SARS-CoV-2 infection, and outcomes were measured for these groups. The primary outcome was the presence and distribution of opportunistic infections. The secondary outcomes included factors associated with opportunistic infections, based on which a nomogram prediction model was developed and validated.ResultsAfter SARS-CoV-2 infection, the proportion of IBD patients with opportunistic infections by Clostridium difficile (21.31% vs. 14.01%, p = 0.044) and Epstein–Barr virus (13.93% vs. 4.35%, p = 0.001) was significantly higher compared to that before. Conversely, the proportion of patients with hepatitis B virus (3.69% vs. 10.14%, p = 0.006) and herpes simplex virus type I (1.23% vs. 4.35%, p = 0.04) infections was significantly lower after the infection. Additionally, pre-SARS-CoV-2 infection factors associated with opportunistic infections in IBD include duration of illness, red blood cell count, the presence of comorbid chronic illnesses, and alcohol consumption, while post-SARS-CoV-2 infection, the primary risk factors involve corticosteroid use, red blood cell count, protein level, and high-sensitivity C-reactive protein.ConclusionAfter the SARS-CoV-2 infection, there has been a shift in the occurrence of opportunistic infections among IBD patients. It might be attributed to the use of corticosteroids and also the strengthening of containment measures, heightened public health awareness, and widespread vaccination.