bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic Nuclear Polarization Provides New Insights into Chromophore Structure in Phytochrome Photoreceptors
Authors: Stöppler Daniel , Song Chen , van Rossum Barth‐Jan , Geiger Michel‐Andreas , Lang Christina , Mroginski Maria‐Andrea , Jagtap Anil Pandurang , Sigurdsson Snorri Th. , Matysik Jörg , Hughes Jon , Oschkinat Hartmut 
Published: November 2016

AbstractPhytochromes are red/far‐red photochromic photoreceptors acting as master regulators of development in higher plants, thereby controlling transcription of about 20 % of their genes. Light‐induced isomerization of the bilin chromophore leads to large rearrangements in protein structure, whereby the role of protonation dynamics and charge distribution is of particular interest. To help unravel the inherent mechanisms, we present two‐dimensional dynamic nuclear polarization (DNP) enhanced solid‐state magic‐angle spinning (MAS) NMR spectra of the functional sensory module of the cyanobacterial phytochrome Cph1. To this end, the pyrrole ring nitrogen signals were assigned unequivocally, enabling us to locate the positive charge of the phycocyanobilin (PCB) chromophore. To help analyze proton exchange pathways, the proximity of PCB ring nitrogen atoms and functionally relevant H2O molecules was also determined. Our study demonstrates the value of DNP in biological solid‐state MAS NMR spectroscopy.
Metal‐Free Enantioselective Oxidative Arylation of Alkenes: Hypervalent‐Iodine‐Promoted Oxidative C−C Bond Formation
Authors: Shimogaki Mio , Fujita Morifumi , Sugimura Takashi 
Published: November 2016

AbstractThe enantioselective oxyarylation of (E)‐6‐aryl‐1‐silyloxylhex‐3‐ene was achieved using a lactate‐based chiral hypervalent iodine(III) reagent in the presence of boron trifluoride diethyl etherate. The silyl ether promotes the oxidative cyclization, and enhances the enantioselectivity. In addition, the corresponding aminoarylation was achieved.
Template‐Free Supracolloidal Self‐Assembly of Atomically Precise Gold Nanoclusters: From 2D Colloidal Crystals to Spherical Capsids
Authors: Nonappa  , Lahtinen Tanja , Haataja Johannes. S. , Tero Tiia‐Riikka , Häkkinen Hannu , Ikkala Olli 
Published: November 2016

AbstractWe report supracolloidal self‐assembly of atomically precise and strictly monodisperse gold nanoclusters involving p‐mercaptobenzoic acid ligands (Au102‐pMBA44) under aqueous conditions into hexagonally packed monolayer‐thick two‐dimensional facetted colloidal crystals (thickness 2.7 nm) and their bending to closed shells leading to spherical capsids (d ca. 200 nm), as controlled by solvent conditions. The 2D colloidal assembly is driven in template‐free manner by the spontaneous patchiness of the pMBA ligands around the Au102‐pMBA44 nanoclusters preferably towards equatorial plane, thus promoting inter‐nanocluster hydrogen bonds and high packing to planar sheets. More generally, the findings encourage to explore atomically precise nanoclusters towards highly controlled colloidal self‐assemblies.
Investigation on miscibility behaviors of elastin‐like polypentapeptide blends with polyvinyl alcohol in aqueous and solid state
Authors: Mahesh B. , Nanjundaswamy G. S. , Channe Gowda D. , Siddaramaiah  
Published: November 2016

ABSTRACTThe polypentapeptide of elastin, poly(GVGVP) has shown remarkable properties and have great potential for biomedical applications. To explore its applications further, the miscibility behaviors of poly(vinyl alcohol) (PVA)/poly(GVGVP) blends have been explored in a very broad composition range. The miscibility behaviors of these blends have been investigated in both solution and solid state using various analytical techniques, including ultrasonic velocity, density, refractive index (RI) and viscosity techniques at 25  °C. The results confirmed the miscibility of the blend up to 40% of the polypeptide. This was further supported by Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44624.
Macroporous hydrogels based on chitosan derivatives: Preparation, characterization, and in vitro evaluation
Authors: Demina Tatiana S. , Zaytseva‐Zotova Daria S. , Akopova Tatiana A. , Zelenetskii Alexander N. , Markvicheva Elena A. 
Published: November 2016

ABSTRACTChitosan‐based hydrogels are considered as promising biomaterials for tissue engineering. Biological properties of chitosan could be significantly improved by modification of its chemical structure. This study was aimed at characterizing macroporous hydrogels fabricated by freeze‐drying technique from chitosan, which has been N‐acetylated by 2,2‐bis(hydroxymethyl)propionic acid or l,d‐lactide. The nature of the acetylated agent was shown to significantly affect hydrogels morphology, swelling behavior, zeta‐potential, and protein sorption as well as their degradation by lysozyme. According to scanning electron and confocal laser scanning microscopy, the hydrogels possessed interconnected macroporous network that facilitated cells penetration into the interior regions of the hydrogel. Chemical modification of chitosan significantly influenced L929 cell growth behavior on hydrogel compared to the non‐modified chitosan. The proposed chemical strategy for modification of chitosan could be considered as promising approach for improvement of chitosan hydrogels. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44651.
Synthesis of tris(2‐hydroxyethyl) isocyanurate homopolymer and its application in intumescent flame retarded polypropylene
Authors: Gao Shang , Zhao Xuan , Liu Gousheng 
Published: November 2016

ABSTRACTA macromolecular homopolymer (named as Homo‐THEIC) was synthesized through self‐etherification of tris(2‐hydroxyethyl) isocyanurate (THEIC) molecules and used as charring agent. Its chemical structure was characterized by FTIR and 13C‐NMR. The charring agent was mixed with ammonium polyphosphate (APP) and applied in flame retarded polypropylene (PP). Results of UL‐94, LOI, and cone calorimeter test showed that the LOI of flame retarded PP can reach 32.8% and UL‐94 V‐0 rating can be achieved at 30 wt % loading. The heat release rate and smoke production rate during the combustion of PP were substantially reduced. TGA results indicated that the synergistic effect between APP and Homo‐THEIC existed and the addition of intumescent flame retardant (IFR) dramatically enhanced the thermal stability of PP. According to the results of TGA, SEM, TG‐FTIR, FTIR, and Raman, the char forming process of IFR can be separated into three stages: the formation of viscous phosphate ester (Tonset−330 °C), the expanding process along with the decomposition of phosphate ester and the release of a large amount of gases (330–480 °C), and the final formation of graphitic‐like char without any expanding feature (480–670 °C). © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44663.
Advances in Reconstructing Archaeological Magnetic Signals; an Algorithm for Filtering Noise due to the Ploughing Effect
Authors: Noviello Mariangela , Ciminale Marcello , Del Gaudio Vincenzo , Amoruso Leonardo 
Published: November 2016

AbstractArchaeological remains are very often buried under uneven soil of agricultural fields crossed by rather parallel furrows and ridges. Consequently, ploughing in a magnetic survey might produce a repetitive, quite regular, linear noise in the data, which could impede optimal recovery of the archaeological magnetic anomalies; depending on the acquisition line orientation, this noise may show as an oblique, vertical or horizontal pattern in the magnetic maps.Several studies have tested and verified methods for oblique ploughing minimization, but, to our knowledge, no procedures regarding the vertical and horizontal types of noise have been published.The present research proposes a procedure to filter each type of ploughing effect through an algorithm working in the wavenumber domain. The procedure produces images with a considerable reduction of the noise in any direction, resulting in enhanced visibility and readability of the archaeological anomalies. Copyright © 2016 John Wiley & Sons, Ltd.
Preparation of Heteroaromatic (Aryl)iodonium Imides as I−N Bond‐Containing Hypervalent Iodine
Authors: Ishida Kazuma , Togo Hideo , Moriyama Katsuhiko 
Published: November 2016

AbstractHypervalent iodine(III) compounds containing iodine–nitrogen bonds are very attractive amination reagents in organic synthesis. Heteroaromatic (aryl)iodonium imides containing a iodine–nitrogen bond and a hypervalent iodine(III) atom were prepared from heteroarenes, bis(sulfon)imides and (diacetoxyiodo)arenes under mild conditions. These compounds were stable under air and in organic solvents, and could be easily purified by precipitation. X‐ray crystal structure analysis indicated that the structure of N‐pivaloyl indolyl(phenyl)iodonium bis(tosyl)imides and N‐pivaloyl indolyl(2‐butoxyphenyl)iodonium bis(tosyl)imides was a dimer with a T‐shaped geometry at the iodine atom linked to an indole group and a bis(tosyl)imide by a monomer unit. Moreover, the use of substituted iodoarenes facilitated the purification of some of the heteroaromatic (aryl)iodonium imides.
Visible‐Light‐Promoted AuI to AuIII Oxidation in Triazol‐5‐ylidene Complexes
Authors: Mendoza‐Espinosa Daniel , Rendón‐Nava David , Alvarez‐Hernández Alejandro , Angeles‐Beltrán Deyanira , Negrón‐Silva Guillermo E. , Suárez‐Castillo Oscar R. 
Published: November 2016

AbstractReaction of triazolium precursors [MIC(CH2)n‐ H+]I− (n=1–3) with potassium hexamethyldisilazane (KHMDS) and AuCl(SMe2) generates the gold(I) complexes of the type MIC(CH2)n⋅AuI. Visible light exposure of the latter complexes promotes a spontaneous disproportionation process rendering gold(III) complexes of the type [{MIC(CH2)n}2⋅AuI2]+I−. Both the AuI and AuIII complex series were tested in the catalytic hydrohydrazination of terminal alkynes using hydrazine as nitrogen source.
A simplified procedure for antibody engineering by yeast surface display: Coupling display levels and target binding by ribosomal skipping
Authors: Grzeschik Julius , Hinz Steffen C. , Könning Doreen , Pirzer Thomas , Becker Stefan , Zielonka Stefan , Kolmar Harald 
Published: November 2016

AbstractYeast surface display is a valuable, widely used method for protein engineering. However, current yeast display applications rely on the staining of epitope tags in order to verify full‐length presentation of the protein of interest on the cell surface. We aimed at developing a modified yeast display approach that relies on ribosomal skipping, thereby enabling the translation of two proteins from one open reading frame and, in that manner, generating an intracellular fluorescence signal. This improved setup is based on a 2A sequence that is encoded between the protein to be displayed and a gene for green fluorescent protein (GFP). The intracellular GFP fluorescence signal of yeast cells correlates with full‐length protein presentation and omits the need for the immunofluorescence detection of epitope tags. For method validation, shark‐derived IgNAR variable domains (vNAR) were subjected to affinity maturation using the 2A‐GFP system. Yeast library screening of full‐length vNAR variants which were detected via GFP expression yielded the same high‐affinity binder that had previously been isolated by our group using the conventional epitope tag‐based display format. The presented method obviates the need for additional immunofluorescence cell staining, offering an easy and cost‐friendly alternative to conventional epitope tag detections.
PDE3 inhibition with enoximone as first‐line therapy for severe persistent pulmonary hypertension of the newborn during neonatal transport: a case report
Authors: van der Lee Robin , Peels Barbara , Koopman‐Esseboom Corine 
Published: November 2016

Key Clinical MessageSevere Persistent pulmonary hypertension of the newborn (PPHN) can be effectively treated with a PDE3 inhibitor as first‐line treatment during neonatal transport when iNO is not readily available. Starting iNO as soon as possible is strongly advised because of the complementary actions of both therapeutics.
Highly Efficient Palladium‐Catalyzed Allylic Alkylation of Cyanoacetamides with Controllable and Chemoselective Mono‐ and Double Substitutions
Authors: Gao Pei‐Sen , Li Ning , Zhang Jin‐Lei , Zhu Zhuang‐Li , Gao Zi‐Wei , Sun Hua‐Ming , Zhang Wei‐Qiang , Xu Li‐Wen 
Published: November 2016

AbstractThe front cover artwork for Issue 22/2016 is provided by Zi‐Wei Gao and Li‐Wen Xu's group of Shaanxi Normal University. The image shows a novel palladium catalyst system combined with a triazine‐modified new phosphine ligand that was developed successfully for the catalytic allylic alkylation of various cyanoacetamides. See the Full Paper itself at http://dx.doi.org/10.1002/cctc.201601021.
One‐Pot Procedure for the Synthesis of 1,5‐Benzodiazepines from N‐Allyl‐2‐bromoanilines
Authors: Weers Marco , Lühning Lars H. , Lührs Vanessa , Brahms Christian , Doye Sven 
Published: November 2016

AbstractA new one‐pot procedure that includes an initial titanium‐catalyzed intermolecular hydroaminoalkylation of N‐allyl‐2‐bromoanilines with N‐methylanilines and a subsequent intramolecular Buchwald–Hartwig amination directly gives access to pharmacologically relevant 1,5‐benzodiazepines. The process takes advantage of the excellent regioselectivity of the initial hydroaminoalkylation performed in the presence of a titanium mono(formamidinate) catalyst and the fact that the exclusively formed branched hydroaminoalkylation products can only undergo palladium‐catalyzed cyclization to 1,5‐benzodiazepines.
Bifunctional Molecular Photoswitches Based on Overcrowded Alkenes for Dynamic Control of Catalytic Activity in Michael Addition Reactions
Authors: Pizzolato Stefano F. , Collins Beatrice S. L. , van Leeuwen Thomas , Feringa Ben L. 
Published: November 2016

AbstractThe emerging field of artificial photoswitchable catalysis has recently shown striking examples of functional light‐responsive systems allowing for dynamic control of activity and selectivity in organocatalysis and metal‐catalysed transformations. While our group has already disclosed systems featuring first generation molecular motors as the switchable central core, a design based on second generation molecular motors is lacking. Here, the syntheses of two bifunctionalised molecular switches based on a photoresponsive tetrasubstituted alkene core are reported. They feature a thiourea substituent as hydrogen‐donor moiety in the upper half and a basic dimethylamine group in the lower half. This combination of functional groups offers the possibility for application of these molecules in photoswitchable catalytic processes. The light‐responsive central cores were synthesized by a Barton–Kellogg coupling of the prefunctionalized upper and lower halves. Derivatization using Buchwald–Hartwig amination and subsequent introduction of the thiourea substituent afforded the target compounds. Control of catalytic activity in the Michael addition reaction between (E)‐3‐bromo‐β‐nitrostyrene and 2,4‐pentanedione is achieved upon irradiation of stable‐(E) and stable‐(Z) isomers of the bifunctional catalyst 1. Both isomers display a decrease in catalytic activity upon irradiation to the metastable state, providing systems with the potential to be applied as ON/OFF catalytic photoswitches.
Findung und Charakterisierung von Adsorbentien zur CO2‐Adsorption aus feuchten Gasströmen
Authors: Fickinger Daniel , Castro‐Gonzales Lenny , Kimmerle Klaus , Schippert Egbert 
Published: November 2016

AbstractMittels Literaturrecherche wurden Adsorbentien identifiziert, die zur physikalischen Adsorption von anthropogenem CO2 aus feuchten Gasströmen geeignet sind. Amindotierte, Silica‐basierte Adsorbentien waren am besten geeignet. Durchbruchskurven von CO2 und Wasserdampf im Gemisch an diesen wurden gemessen. Das amindotierte Silicagel Tri‐PE‐MCM‐41 zeigte sehr gute Eigenschaften bzgl. der CO2‐Adsorption aus feuchter Luft. Die thermodynamische Auswertung der Reinstoffadsorption von CO2 mittels des ALIc‐Modells zeigt, dass die stärksten Bindungskräfte zwischen Adsorbens und CO2‐Molekül in Tri‐PE‐MCM‐41 vorliegen.
Impact of prior permanent pacemaker on long‐term clinical outcomes of patients undergoing percutaneous coronary intervention
Authors: Li Yan‐Jie , Zhang Wei‐Wei , Yang Xiao‐Xiao , Li Ning , Qiu Xing‐Biao , Qu Xin‐Kai , Fang Wei‐Yi , Yang Yi‐Qing , Li Ruo‐Gu 
Published: November 2016

BackgroundThe impact of permanent pacemaker (PPM) on long‐term clinical outcomes of patients undergoing percutaneous coronary intervention (PCI) has not been studied.Hypothesis
PPM may increase heart failure (HF) burden on patients undergoing PCI.MethodsWe recruited consecutive patients undergoing PCI and carried out a nested case–control study. Patients with confirmed PPM undergoing first PCI were identified and matched by age and sex in 1:1 fashion to patients without PPM undergoing first PCI. Clinical data were collected and analyzed. The primary endpoint outcomes were all‐cause mortality and hospitalization for HF.ResultsThe final analysis included 156 patients. The mean follow‐up period was 4.6 ± 2.9 years. The overall all‐cause mortality was 21.15%, without significant difference between the 2 groups (21.79% vs 20.51%; P = 0.85). However, the rate of HF‐related hospitalization was significantly higher in patients with PPM than in controls (26.92% vs 10.26%; P = 0.008). After adjustment for hypertension, type 2 diabetes mellitus, hyperlipidemia, chronic kidney disease, stroke, left ventricular ejection fraction, brain natriuretic peptide, and acute coronary syndrome (ACS), PCI patients with PPM were still associated with a greater hospitalization rate for HF (odds ratio: 4.31, 95% confidence interval: 0.94‐19.80, P = 0.061). Further analysis in the ACS subgroup showed VVI‐mode pacing enhanced the risk for HF‐associated hospitalization (adjusted odds ratio: 8.27, 95% confidence interval: 1.37‐49.75, P = 0.02).Conclusions
PPM has no effect on all‐cause mortality in patients undergoing first PCI but significantly increases the HF‐associated hospitalization rate, especially in ACS patients.
Carbazole Aminoalcohols Induce Antiproliferation and Apoptosis of Human Tumor Cells by Inhibiting Topoisomerase I
Authors: Wang Weisi , Sun Xiao , Sun Deheng , Li Shizhu , Yu Yang , Yang Tingyuan , Yao Junmin , Chen Zhuo , Duan Liping 
Published: November 2016

AbstractNovel carbazole aminoalcohols were designed and synthesized as anticancer agents. Among them, alkylamine‐chain‐substituted compounds showed the most promising antiproliferative activity, with IC50 values in the single‐digit micromolar range against two human tumor cell lines. Topoisomerase I (topo I) is likely to be one of the targets of these compounds. Results of comet assays and molecular docking indicate that the representative compounds may act as topo I poisons, causing single‐strand DNA damage by stabilizing the topo I–DNA cleavage complex. In particular, the most potent compound, 1‐(butylamino)‐3‐(3,6‐dichloro‐9H‐carbazol‐9‐yl)propan‐2‐ol (6), was shown to be able to induce G2‐phase cell‐cycle arrest and apoptosis in HeLa cells.
N‐Arylsulfonyl Indolines as Retinoic Acid Receptor‐Related Orphan Receptor γ (RORγ) Agonists
Authors: Doebelin Christelle , Patouret Rémi , Garcia‐Ordonez Ruben D. , Chang Mi Ra , Dharmarajan Venkatasubramanian , Kuruvilla Dana S. , Novick Scott J. , Lin Li , Cameron Michael D. , Griffin Patrick R. , Kamenecka Theodore M. 
Published: November 2016

AbstractThe nuclear retinoic acid receptor‐related orphan receptor γ (RORγ; NR1F3) is a key regulator of inflammatory gene programs involved in T helper 17 (TH17) cell proliferation. As such, synthetic small‐molecule repressors (inverse agonists) targeting RORγ have been extensively studied for their potential as therapeutic agents for various autoimmune diseases. Alternatively, enhancing TH17 cell proliferation through activation (agonism) of RORγ may boost an immune response, thereby offering a potentially new approach in cancer immunotherapy. Herein we describe the development of N‐arylsulfonyl indolines as RORγ agonists. Structure–activity studies reveal a critical linker region in these molecules as the major determinant for agonism. Hydrogen/deuterium exchange coupled to mass spectrometry (HDX‐MS) analysis of RORγ–ligand complexes help rationalize the observed results.
Elimination of Power Overshoot at Bioanode through Assistance Current in Microbial Fuel Cells
Authors: Kim Bongkyu , An Junyeong , Chang In Seop 
Published: November 2016

AbstractThe power overshoot generated by electron depletion in microbial fuel cells (MFCs) was characterized in this study. Various causes of power overshoot, identified in previous studies, are discussed in terms of their plausible contributions to electron depletion. We found that power overshoot occurred if the anodic overpotential generated by electron depletion exceeded the cathodic overpotential. The introduction of assistance current from anode connections, which ameliorated the electron depletion in the MFCs, immediately eliminated the power overshoot. As a result, if the electron production at the anode exceeded electron reduction at the cathode, a power overshoot was not generated. The results revealed that introducing assistance current supplied from an additional anode to the limited anode eliminated power overshoot. The power overshoot is not generated by kinetic limitation at the cathode; it is only generated by the kinetic limitation at the anode. The mechanism underlying power overshoot should be considered in the design of MFCs to improve reliability, particularly in scaled‐up plant applications. The proposed technique is more practical than previously proposed methods.
A near‐Nyquist rate transmission: A new minimum‐bandwidth direct‐sequence code division multiple access scheme
Authors: Lin Wei‐Lun 
Published: November 2016

SummaryBandlimited direct‐sequence code division multiple access (DS‐CDMA) attracts much attention for its compact spectrum and the ability to suppress inter‐symbol interference. Among the various bandlimited DS‐CDMA systems available, minimum‐bandwidth DS‐CDMA (MB‐DS‐CDMA) is the only realizable Nyquist rate transmission system. But, MB‐DS‐CDMA only applies to certain kinds of spreading codes. Accordingly, this study proposes a modified DS‐CDMA structure which extends the application of MB‐DS‐CDMA to all common spreading codes at the expense of a negligible reduction in the transmission rate. Additionally, the bit error rate of the proposed schemes adopting either single‐user or multi‐user detection receiver is analyzed and compared with that of the commonly‐used raised‐cosine‐pulsed DS‐CDMA over multipath fading channels. The numerical results show that given a sufficiently large number of users, the bit error rate performance of modified MB‐DS‐CDMA is comparable to that of the raised‐cosine‐pulsed DS‐CDMA scheme; meanwhile, the realizable modified MB‐DS‐CDMA approaches the ultimate transmission rate.
Mating and longevity in ant males
Authors: Metzler Sina , Heinze Jürgen , Schrempf Alexandra 
Published: November 2016

AbstractAcross multicellular organisms, the costs of reproduction and self‐maintenance result in a life history trade‐off between fecundity and longevity. Queens of perennial social Hymenoptera are both highly fertile and long‐lived, and thus, this fundamental trade‐off is lacking. Whether social insect males similarly evade the fecundity/longevity trade‐off remains largely unstudied. Wingless males of the ant genus Cardiocondyla stay in their natal colonies throughout their relatively long lives and mate with multiple female sexuals. Here, we show that Cardiocondyla obscurior males that were allowed to mate with large numbers of female sexuals had a shortened life span compared to males that mated at a low frequency or virgin males. Although frequent mating negatively affects longevity, males clearly benefit from a “live fast, die young strategy” by inseminating as many female sexuals as possible at a cost to their own survival.