bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequency of Risk‐Related News Media Messages in 2016 Coverage of Zika Virus
Authors: Sell Tara Kirk , Watson Crystal , Meyer Diane , Kronk Marissa , Ravi Sanjana , Pechta Laura E. , Lubell Keri M. , Rose Dale A. 
Published: January 2018

AbstractNews media plays a large role in the information the public receives during an infectious disease outbreak, and may influence public knowledge and perceptions of risk. This study analyzed and described the content of U.S. news media coverage of Zika virus and Zika response during 2016. A random selection of 800 Zika‐related news stories from 25 print and television news sources was analyzed. The study examined 24 different messages that appeared in news media articles and characterized them using theories of risk perception as messages with characteristics that could increase perception of risk (risk‐elevating messages; n = 14), messages that could decrease perception of risk (risk‐minimizing messages; n = 8), or messages about travel or testing guidance (n = 2). Overall, 96% of news stories in the study sample contained at least one or more risk‐elevating message(s) and 61% contained risk‐minimizing message(s). The frequency of many messages changed after local transmission was confirmed in Florida, and differed between sources in locations with or without local transmission in 2016. Forty percent of news stories included messages about negative potential outcomes of Zika virus infection without mentioning ways to reduce risk. Findings from this study may help inform current federal, state, and local Zika responses by offering a detailed analysis of how news media are covering the outbreak and response activities as well as identifying specific messages appearing more or less frequently than intended. Findings identifying the types of messages that require greater emphasis may also assist public health communicators in responding more effectively to future outbreaks.
Dialysis access procedure training for the nephrologist
Authors: Waheed Sana , Maursetter Laura , Yevzlin Alexander 
Published: January 2018

AbstractHistorically, the placement and maintenance of dialysis access has been an integral part of nephrology training. However, in recent years, a big debate has ensued regarding whether this should be limited to trainees’ understanding and counseling the patients regarding indications, alternatives, risks and possible complications of these procedures or should it actually involve more of a hands‐on experience for the trainees. Some of the barriers in making these procedures a requirement across the board are the lack of standardization of procedural training across various training programs and the absence of consensus on what achieving competency in these procedures looks like. However, in the era of declining interest in nephrology, giving up “ownership” of nephrology procedures and increasing reliance on other sub specialties might be a deterrent in attracting residents to this field; we have to make a concerted effort to increase the exposure and opportunities for the trainees to perform these procedures. Moreover, we need to emphasize the implementation of a curriculum for nephrology fellows to evaluate access properly in order to decrease the burden of access related complications. Lastly, we need to continue working towards a more structured curriculum for a dedicated interventional nephrology fellowship for trainees who want to focus on procedures for their long‐term career goals.
Complete Genome Sequence of
Photobacterium leiognathi
Strain JS01
Authors: Soh Justin Y. K. , Russell Colin W. , Fenlon Shannon N. , Chen Swaine L. 
Published: January 2018

ABSTRACT

Photobacterium leiognathi
is a bioluminescent symbiont of fish of the
Leiognathidae
family. Here, we present the full-genome sequence of
P. leiognathi
strain JS01, a strain isolated from a nonluminescent
Loligo
sp. squid of Singaporean origin. No finished genome sequence of this species is currently publicly available.
Draft Genome Sequence of USA100 Methicillin-Resistant
Staphylococcus aureus
Strain 209
Authors: Chen Yingfeng , Crosby Heidi A. , Oosthuysen Wilhelm F. , Diekema Daniel J. , Kelley Scott T. , Horswill Alexander R. 
Published: January 2018

ABSTRACT

USA100 strains are significant contributors to the overall burden of health care-associated methicillin-resistant
Staphylococcus aureus
(MRSA) infections. Strain 209 is a representative MRSA isolate that serves as a model organism for
agr
type II studies and USA100 virulence assessments. We present a draft genome sequence of this strain.
Whole-Genome Sequencing of Six
Borrelia miyamotoi
Clinical Strains Isolated in Russia
Authors: Kuleshov Konstantin V. , Koetsveld Joris , Goptar Irina A. , Markelov Mikhail L. , Kolyasnikova Nadezhda M. , Sarksyan Denis S. , Toporkova Marina G. , Kirdyashkina Nina P. , Shipulin German A. , Hovius Joppe W. , Platonov Alexander E. 
Published: January 2018

ABSTRACT

Here, we report the whole-genome sequence of six clinical
Borrelia miyamotoi
isolates from the Russian Federation. Using two independent next-generation sequencing platforms, we determined the complete sequence of the chromosome and several plasmids. All strains have an Asian genotype with 99.8% chromosome nucleotide similarity with
B. miyamotoi
strain FR64b.
Draft Genome Sequence of a Red-Pigmented
Janthinobacterium
sp. Native to the Hudson Valley Watershed
Authors: O’Brien Kelsey , Perron Gabriel G. , Jude Brooke A. 
Published: January 2018

ABSTRACT

Water samples from the Hudson Valley watershed indicate that the area is host to many violacein-producing bacterial isolates. Here, we report the draft whole-genome sequence of
Janthinobacterium
sp. strain BJB412, an isolate lacking violacein production yet containing genes responsible for prodigiosin, biofilm production, and quorum sensing, like its purple-pigmented counterparts.
Complete Genome Sequences of Three
Streptococcus agalactiae
Serotype Ia Isolates Obtained from Disease Outbreaks in Nile Tilapia (
Oreochromis niloticus
)
Authors: Jaglarz Anita , Gurgul Artur , Leigh William J. , Costa Janina Z. , Thompson Kim D. 
Published: January 2018

ABSTRACT

This paper describes the whole-genome sequences for three
Streptococcus agalactiae
serotype Ia isolates. The isolates were recovered from the brains of clinically sick tilapia,
Oreochromis niloticus
, that were suffering from streptococcosis. One isolate was from tilapia in the United States and the other two from fish in China.
Host Tumor Suppressor p18INK4cFunctions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis
Authors: Niemeyer Brian F. , Oko Lauren M. , Medina Eva M. , Oldenburg Darby G. , White Douglas W. , Cool Carlyne D. , Clambey Eric T. , van Dyk Linda F. 
Published: January 2018

ABSTRACTGammaherpesviruses are common viruses associated with lifelong infection and increased disease risk. Reactivation from latency aids the virus in maintaining infection throughout the life of the host and is responsible for a wide array of disease outcomes. Previously, we demonstrated that the virus-encoded cyclin (v-cyclin) of murine gammaherpesvirus 68 (γHV68) is essential for optimal reactivation from latency in normal mice but not in mice lacking the host tumor suppressor p18INK4c(p18). Whether p18 plays a cell-intrinsic or -extrinsic role in constraining reactivation remains unclear. Here, we generated recombinant viruses in which we replaced the viral cyclin with the cellular p18INK4cgene (p18KI) for targeted expression of p18, specifically within infected cells. We find that the p18KI virus is similar to the cyclin-deficient virus (cycKO) in lytic infection, establishment of latency, and infected cell reservoirs. While the cycKO virus is capable of reactivation in p18-deficient mice, expression of p18 from the p18KI virus results in a profound reactivation defect. These data demonstrate that p18 limits reactivation within latently infected cells, functioning in a cell-intrinsic manner. Further, the p18KI virus showed greater attenuation of virus-induced lethal pneumonia than the cycKO virus, indicating that p18 could further restrict γHV68 pathogenesis even in p18-sufficient mice. These studies demonstrate that host p18 imposes the requirement for the viral cyclin to reactivate from latency by functioning in latently infected cells and that p18 expression is associated with decreased disease, thereby identifying p18 as a compelling host target to limit chronic gammaherpesvirus pathogenesis.IMPORTANCEGammaherpesviruses are ubiquitous viruses associated with multiple malignancies. The propensity to cycle between latency and reactivation results in an infection that is never cleared and often difficult to treat. Understanding the balance between latency and reactivation is integral to treating gammaherpesvirus infection and associated disease outcomes. This work characterizes the role of a novel inhibitor of reactivation, host p18INK4c, thereby bringing more clarity to a complex process with significant outcomes for infected individuals.
A Role for Myosin Va in Human Cytomegalovirus Nuclear Egress
Authors: Wilkie Adrian R. , Sharma Mayuri , Pesola Jean M. , Ericsson Maria , Fernandez Rosio , Coen Donald M. 
Published: January 2018

ABSTRACT
Herpesviruses replicate and package their genomes into capsids in replication compartments within the nuclear interior. Capsids then move to the inner nuclear membrane for envelopment and release into the cytoplasm in a process called nuclear egress. We previously found that nuclear F-actin is induced upon infection with the betaherpesvirus human cytomegalovirus (HCMV) and is important for nuclear egress and capsid localization away from replication compartment-like inclusions toward the nuclear rim. Despite these and related findings, it has not been shown that any specific motor protein is involved in herpesvirus nuclear egress. In this study, we have investigated whether the host motor protein, myosin Va, could be fulfilling this role. Using immunofluorescence microscopy and coimmunoprecipitation, we observed associations between a nuclear population of myosin Va and the viral major capsid protein, with both concentrating at the periphery of replication compartments. Immunoelectron microscopy showed that nearly 40% of assembled nuclear capsids associate with myosin Va. We also found that myosin Va and major capsid protein colocalize with nuclear F-actin. Importantly, antagonism of myosin Va with RNA interference or a dominant negative mutant revealed that myosin Va is important for the efficient production of infectious virus, capsid accumulation in the cytoplasm, and capsid localization away from replication compartment-like inclusions toward the nuclear rim. Our results lead us to suggest a working model whereby human cytomegalovirus capsids associate with myosin Va for movement from replication compartments to the nuclear periphery during nuclear egress.

IMPORTANCE
Little is known regarding how newly assembled and packaged herpesvirus capsids move from the nuclear interior to the periphery during nuclear egress. While it has been proposed that an actomyosin-based mechanism facilitates intranuclear movement of alphaherpesvirus capsids, a functional role for any specific myosin in nuclear egress has not been reported. Furthermore, the notion that an actomyosin-based mechanism facilitates intranuclear capsid movement is controversial. Here we show that human cytomegalovirus capsids associate with nuclear myosin Va and F-actin and that antagonism of myosin Va impairs capsid localization toward the nuclear rim and nuclear egress. Together with our previous results showing that nuclear F-actin is induced upon HCMV infection and is also important for these processes, our results lend support to the hypothesis that nascent human cytomegalovirus capsids migrate to the nuclear periphery via actomyosin-based movement. These results shed light on a poorly understood viral process and the cellular machinery involved.
Rapid Cloning of Novel Rhesus Adenoviral Vaccine Vectors
Authors: Abbink Peter , Kirilova Marinela , Boyd Michael , Mercado Noe , Li Zhenfeng , Nityanandam Ramya , Nanayakkara Ovini , Peterson Rebecca , Larocca Rafael A. , Aid Malika , Tartaglia Lawrence , Mutetwa Tinaye , Blass Eryn , Jetton David , Maxfield Lori F. , Borducchi Erica N. , Badamchi-Zadeh Alexander , Handley Scott , Zhao Guoyan , Virgin Herbert W. , Havenga Menzo J. , Barouch Dan H. 
Published: January 2018

ABSTRACT
Human and chimpanzee adenovirus vectors are being developed to circumvent preexisting antibodies against common adenovirus vectors such as Ad5. However, baseline immunity to these vectors still exists in human populations. Traditional cloning of new adenovirus vaccine vectors is a long and cumbersome process that takes 2 months or more and that requires rare unique restriction enzyme sites. Here we describe a novel, restriction enzyme-independent method for rapid cloning of new adenovirus vaccine vectors that reduces the total cloning procedure to 1 week. We developed 14 novel adenovirus vectors from rhesus monkeys that can be grown to high titers and that are immunogenic in mice. All vectors grouped with the unusual adenovirus species G and show extremely low seroprevalence in humans. Rapid cloning of novel adenovirus vectors is a promising approach for the development of new vector platforms. Rhesus adenovirus vectors may prove useful for clinical development.

IMPORTANCE
To overcome baseline immunity to human and chimpanzee adenovirus vectors, we developed 14 novel adenovirus vectors from rhesus monkeys. These vectors are immunogenic in mice and show extremely low seroprevalence in humans. Rhesus adenovirus vectors may prove useful for clinical development.
Class 1-Selective Histone Deacetylase (HDAC) Inhibitors Enhance HIV Latency Reversal while Preserving the Activity of HDAC Isoforms Necessary for Maximal HIV Gene Expression
Authors: Zaikos Thomas D. , Painter Mark M. , Sebastian Kettinger Nadia T. , Terry Valeri H. , Collins Kathleen L. 
Published: January 2018

ABSTRACT

Combinations of drugs that affect distinct mechanisms of HIV latency aim to induce robust latency reversal leading to cytopathicity and elimination of the persistent HIV reservoir. Thus far, attempts have focused on combinations of protein kinase C (PKC) agonists and pan-histone deacetylase inhibitors (HDIs) despite the knowledge that HIV gene expression is regulated by class 1 histone deacetylases. We hypothesized that class 1-selective HDIs would promote more robust HIV latency reversal in combination with a PKC agonist than pan-HDIs because they preserve the activity of proviral factors regulated by non-class 1 histone deacetylases. Here, we show that class 1-selective agents used alone or with the PKC agonist bryostatin-1 induced more HIV protein expression per infected cell. In addition, the combination of entinostat and bryostatin-1 induced viral outgrowth, whereas bryostatin-1 combinations with pan-HDIs did not. When class 1-selective HDIs were used in combination with pan-HDIs, the amount of viral protein expression and virus outgrowth resembled that of pan-HDIs alone, suggesting that pan-HDIs inhibit robust gene expression induced by class 1-selective HDIs. Consistent with this, pan-HDI-containing combinations reduced the activity of NF-κB and Hsp90, two cellular factors necessary for potent HIV protein expression, but did not significantly reduce overall cell viability. An assessment of viral clearance from
in vitro
cultures indicated that maximal protein expression induced by class 1-selective HDI treatment was crucial for reservoir clearance. These findings elucidate the limitations of current approaches and provide a path toward more effective strategies to eliminate the HIV reservoir.

IMPORTANCE
Despite effective antiretroviral therapy, HIV evades eradication in a latent form that is not affected by currently available drug regimens. Pharmacologic latency reversal that leads to death of cellular reservoirs has been proposed as a strategy for reservoir elimination. Because histone deacetylases (HDACs) promote HIV latency, HDAC inhibitors have been a focus of HIV cure research. However, many of these inhibitors broadly affect multiple classes of HDACs, including those that promote HIV gene expression (class 1 HDACs). Here, we demonstrate that targeted treatment with class 1-selective HDAC inhibitors induced more potent HIV latency reversal than broadly acting agents. Additionally, we provide evidence that broadly acting HDIs are limited by inhibitory effects on non-class 1 HDACs that support the activity of proviral factors. Thus, our work demonstrates that the use of targeted approaches to induce maximum latency reversal affords the greatest likelihood of reservoir elimination.
Acute sedation-associated complications in GI endoscopy (ProSed 2 Study): results from the prospective multicentre electronic registry of sedation-associated complications
Authors: Behrens Angelika , Kreuzmayr Anton , Manner Hendrik , Koop Herbert , Lorenz Albrecht , Schaefer Claus , Plauth Mathais , Jetschmann Jens-Uwe , von Tirpitz Christian , Ewald Marcus , Sackmann Michael , Renner Wanja , Krüger Martin , Schwab Dieter , Hoffmann Werner , Engelke Olaf , Pech Oliver , Kullmann Frank , Pampuch Sonja , Lenfers Berthold , Weickert Uwe , Schilling Dieter , Boehm Stephan , Beckebaum Susanne , Cicinnati Vito , Erckenbrecht Joachim F , Dumoulin Franz Ludwig , Benz Claus , Rabenstein Thomas , Haltern Georg , Balsliemke Martin , de Mas Christian , Kleber Gerhard , Pehl Christian , Vogt Christoph , Kiesslich Ralf , Fischbach Wolfgang , Koop Irmtraut , Kuehne Jens , Breidert Matthias , Sass Nils Lennart , May Andrea , Friedrich Christian , Veitt Ronni , Porschen Rainer , Ellrichmann Mark , Arlt Alexander , Schmitt Wolfgang , Dollhopf Markus , Schmidbaur Werner , Dignass Axel , Schmitz Volker , Labenz J , Kaiser Gernot , Krannich Alexander , Barteska Nico , Ell Christian 
Published: January 2018

ObjectivesSedation has been established for GI endoscopic procedures in most countries, but it is also associated with an added risk of complications. Reported complication rates are variable due to different study methodologies and often limited sample size.DesignsAcute sedation-associated complications were prospectively recorded in an electronic endoscopy documentation in 39 study centres between December 2011 and August 2014 (median inclusion period 24 months). The sedation regimen was decided by each study centre.ResultsA total of 368 206 endoscopies was recorded; 11% without sedation. Propofol was the dominant drug used (62% only, 22.5% in combination with midazolam). Of the sedated patients, 38 (0.01%) suffered a major complication, and overall mortality was 0.005% (n=15); minor complications occurred in 0.3%. Multivariate analysis showed the following independent risk factors for all complications: American Society of Anesthesiologists class >2 (OR 2.29) and type and duration of endoscopy. Of the sedation regimens, propofol monosedation had the lowest rate (OR 0.75) compared with midazolam (reference) and combinations (OR 1.0–1.5). Compared with primary care hospitals, tertiary referral centres had higher complication rates (OR 1.61). Notably, compared with sedation by a two-person endoscopy team (endoscopist/assistant; 53.5% of all procedures), adding another person for sedation (nurse, physician) was associated with higher complication rates (ORs 1.40–4.46), probably due to higher complexity of procedures not evident in the multivariate analysis.ConclusionsThis large multicentre registry study confirmed that severe acute sedation-related complications are rare during GI endoscopy with a very low mortality. The data are useful for planning risk factor-adapted sedation management to further prevent sedation-associated complications in selected patients.Trial registration numberDRKS00007768; Pre-results.
A Toolkit for Snow-Cover Area Calculation and Display Based on the Interactive Multisensor Snow and Ice Mapping System and an Example for the Tibetan Plateau Region
Authors: Tucker Tyler C. , Shen Samuel S. P. 
Published: January 2018

This research develops a toolkit for snow-cover area calculation and display (SACD) based on the Interactive Multisensor Snow and Ice Mapping System (IMS). The paper uses the Tibetan Plateau region as an example to describe the toolkit’s method, results, and usage. The National Snow and Ice Data Center (NSIDC) provides to the public IMS a well-used system for monitoring the snow and ice cover. The newly developed toolkit is based on a simple shoe-lace formula for a grid box area on a sphere and can be conveniently used to calculate the total area of snow cover given the IMS data. The toolkit has been made available as an open source Python software on GitHub. The toolkit generates the time series of the daily snow-covered area for any region over the Northern Hemisphere from 4 February 1997. The toolkit also creates maps showing snow and ice coverage with an elevation background. The Tibetan Plateau (TP) region [Formula: see text]–[Formula: see text]N)[Formula: see text][Formula: see text]–[Formula: see text]E) is used as an example to demonstrate our work on SACD. The IMS products at 24, 4, and 1[Formula: see text]km resolutions include each grid’s latitude and longitude coordinates that are used to calculate the grid box’s area using the shoe-lace formula. The total TP area calculated by the sum of the areas of all the grid boxes approximates the true spherical TP surface area bounded by [Formula: see text]–[Formula: see text]N) [Formula: see text]–[Formula: see text]E) with a difference 0.046% for the 24[Formula: see text]km grid and 0.033% for the 4[Formula: see text]km grid. The differences in the snow-cover area reported by the 24[Formula: see text]km and 4[Formula: see text]km grids vary between [Formula: see text]% and 6.24%. The temporal variations of the daily TP snow cover are displayed in time series from 4 February 1997 to present with 4[Formula: see text]km and 24[Formula: see text]km resolutions.
Targeting HER2 Aberrations in Non–Small Cell Lung Cancer with Osimertinib
Authors: Liu Shengwu , Li Shuai , Hai Josephine , Wang Xiaoen , Chen Ting , Quinn Max M. , Gao Peng , Zhang Yanxi , Ji Hongbin , Cross Darren A.E. , Wong Kwok-Kin 
Published: January 2018

Abstract
Purpose: HER2 (or ERBB2) aberrations, including both amplification and mutations, have been classified as oncogenic drivers that contribute to 2% to 6% of lung adenocarcinomas. HER2 amplification is also an important mechanism for acquired resistance to EGFR tyrosine kinase inhibitors (TKI). However, due to limited preclinical studies and clinical trials, currently there is still no available standard of care for lung cancer patients with HER2 aberrations. To fulfill the clinical need for targeting HER2 in patients with non–small cell lung cancer (NSCLC), we performed a comprehensive preclinical study to evaluate the efficacy of a third-generation TKI, osimertinib (AZD9291).
Experimental Design: Three genetically modified mouse models (GEMM) mimicking individual HER2 alterations in NSCLC were generated, and osimertinib was tested for its efficacy against these HER2 aberrations in vivo.
Results: Osimertinib treatment showed robust efficacy in HER2wt overexpression and EGFR del19/HER2 models, but not in HER2 exon 20 insertion tumors. Interestingly, we further identified that combined treatment with osimertinib and the BET inhibitor JQ1 significantly increased the response rate in HER2-mutant NSCLC, whereas JQ1 single treatment did not show efficacy.
Conclusions: Overall, our data indicated robust antitumor efficacy of osimertinib against multiple HER2 aberrations in lung cancer, either as a single agent or in combination with JQ1. Our study provides a strong rationale for future clinical trials using osimertinib either alone or in combination with epigenetic drugs to target aberrant HER2 in patients with NSCLC. Clin Cancer Res; 24(11); 2594–604. ©2018 AACR.
See related commentary by Cappuzzo and Landi, p. 2470