bluuebunny/crossref_metadata_2025_split huggingface dataset:
Local inflammation at the salmon louse (Lepeophtheirus salmonis) attachment site drives copepodid rejection in Coho Salmon (Oncorhynchus kisutch)
Authors: Sveen Lene , Fast Mark D. , Tengs Torstein , Timmerhaus Gerrit , Vaadal Marianne , Houston Ross D. , Bron James E. , Monaghan Sean J. , Mohammed Haitham H. , Daniels Rose Ruiz , Salisbury Sarah , Robledo Diego , Braceland Mark , Hansen Miroslava , Robinson Nicholas 
Published: September 2024

Abstract

Salmon louse (Lepeophtheirus salmonis) poses significant risks to both wild and farmed salmon populations. Atlantic Salmon (Salmo salar) are highly susceptible to the parasite, while Pacific salmon species such as Pink (Oncorhynchus gorbuscha), Coho (Oncorhynchus kisutch), and Chum (Oncorhynchus keta) salmon exhibit varying levels of resistance. This study explored L. salmonis infestation dynamics in four salmonid species (Atlantic, Pink, Coho, and Chum Salmon) across three challenge trials using the same fish cohort. Infestation was only successful in the third trial, where sedation facilitated parasite attachment. Skin and fin samples were collected at 12, 24, 36, 48, 60, and 168 hours post-infestation (hpi) during the early infestation phase. Results showed low L. salmonis settlement on Chum and Pink Salmon, while Coho Salmon initially had the highest lice counts on fins, followed by Atlantic Salmon. A strong correlation was observed between mucous cell area and susceptibility, with Chum Salmon showing 30-40% mucous cell coverage compared to 10% in Atlantic Salmon. Coho Salmon successfully rejected copepodids after two days, which was linked to an influx of polymorphonuclear cells at attachment sites. This immune response, highlighted by spatial transcriptomics, showed upregulation of host immune markers at the louse-host interface, suggesting that macrophages and neutrophils are key to copepodid rejection. The findings suggest that Coho Salmon’s rapid inflammatory response is crucial in resisting lice and differs significantly from the response in Atlantic Salmon.
Host transcriptome profiling for resistance against Lumpy Skin Disease (LSD)
Authors: Banabazi Mohammad Hossein , Borm Steven , Klingström Tomas , Niazi Adnan , Clercq Kris , Mostin Laurent , Haegeman Andy , Koning Dirk Jan 
Published: September 2024

Abstract

Objective
Lumpy skin disease (LSD) is an acute or subacute systemic viral disease of cattle that shows variation in the response of cattle to LSD virus infection. To better understand the mechanisms underlying this response diversity in field studies and under carefully controlled artificial infections, we studied the differentially expressed genes (DEGs) between two resilient versus three susceptible Holstein bulls before an infection challenge and three time points after that.
Results
The host transcriptome profiling revealed that IL1RAP gene expression could be a potential determinant in distinguishing between resilient and susceptible cattle (padj < 0.05). It was significantly shifted from up-regulated prior to infection to down-regulated three days post-infection in the LSD-resilient cattle. Its expression remained up-regulated among the susceptible cattle post-infection compared to pre-infection. The results showed that seven days post-infection may be a critical time point for LSD infection. The Gene Ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment test showed a few enriched GO terms and pathways relevant to the LSD and the involvement of the IL1RAP gene. This pilot study, with limited statistical power, is the first to investigate bovine gene expression profiling in response to LSDV.
Research on the Recommendation Method of Urban Location Point of Interest Based on DTCN-EFFN-Transformer
Authors: Zhang Jing , Li Bing , Zhang Yao , Xu Yuguang , Li Hongan 
Published: September 2024

Abstract

In recent years, user travel has generated massive mobile data. Recommending the next location of interest not only greatly facilitates user travel, but also provides a corresponding opportunity for merchants to mine potential customers. In view of the fact that the existing urban location POI recommendation does not integrate multiple context information, leading to a series of issues, such as the inability of the recommendation results to accurately meet user needs. This research introduces a method for urban location POI recommendation utilizing the DTCN-EFFN-Transformer. This method combines user preferences, geographic location and popularity of POIs. Firstly, the deep convolution in DTCN simplifies the complexity of input data, so that the dilated causal convolution in TCN can focus more on extracting local features in user check-in records. Secondly , an expansion factor is added to the linear layer of EFFN. By extending the dimension of the middle layer, the nonlinear representation ability of the model is enhanced, thereby the long-distance dependence ability of the model in capturing user check-in records is improved. Experimental results indicate that using the FourSquare-NYC and FourSquare-TKY datasets, this newly introduced method improves Acc@1 index by 10.42% and 6.13% respectively compared with similar representative methods.
General practitioners' management of occupational diseases: a qualitative study in French- speaking Switzerland
Authors: Coline Gaubicher , Staeger Philippe , Danuser Brigitta , Abderhalden-Zellweger Alessia , Peggy Krief , Mediouni Zakia 
Published: September 2024

Abstract

Background
Work-related health problems are a frequent reason for consultation with primary care physicians; however, few studies report on the way general practitioners’ (GPs) approach work-related health issues with their patients.
Aim
To investigate how GPs manage patients' occupational issues.
Methods
Semi-structured interviews were conducted with 12 GPs, transcribed, and analyzed thematically.
Results
GPs do explore their patients' occupational field. However, the data collected are succinct, even though doctors recognize that a high proportion of mental disorders and musculoskeletal disorders may be work-related. Work-related disease insurance issues are very present in GPs everyday practice, with a discomfort regarding these issues. Exchanges with employers are not commonplace but examples show that they can be fruitful. Occupational risk prevention is confined to certain high-risk exposures. When asked to express their difficulties in dealing with patients work-related issues, GPs mention the lack of dedicated time, insurance issues, contact with employers or the lack of it and lack of training. As for resources, GPs mainly identify specialist doctors, but still, only few of them mention occupational physicians.
Conclusions
Some work-related pathologies, whether frequent or serious, are not systematically investigated by GPs. This raises the question of their detection and probable under-reporting. To improve anamnesis, identification, and referral practices in the event of occupational health problems, we suggest a targeted training for GPs, improved networking including stakeholders such as occupational physicians, employers and insurers and revising medical billing regarding work related diseases management both for GPs and occupational physicians.
Effect of electromagnetic transducer design parameters on round-window stimulation in otosclerosis: A nonlinear dynamic analysis
Authors: Liu Houguang , Liu Zhaohai , Liu Jianshu , Lenarz Thomas , Maier Hannes 
Published: September 2024

Abstract

This study presents a novel comprehensive human ear electromagnetic transducer model considering cochlear third windows and middle-ear nonlinear tissue properties, i.e., of the tympanic membrane, the tensor tympani, and the annular ligament, to investigate the effect of the floating mass transducer’s design parameters on round-window stimulation in treating otosclerosis. The model's reliability was confirmed by comparing its predictions of stapes velocity and middle ear transfer function with experimental data. The influences of the electromagnetic transducer’s main design parameters, i.e., excitation frequency, electromechanical coupling coefficient, case mass, magnet mass, support stiffness, and coupler layer stiffness, on the cochlear differential pressure in both normal middle ear and otosclerosis were examined. Additionally, the study explored chaotic motion conditions in relation to these design parameters. The results show that the stimulated cochlear differential pressure in otosclerosis is lower than that in the normal middle ear at different excitation frequencies. A higher electromechanical coupling coefficient contributes to the avoidance of chaotic motion, and the output of the transducer is positively correlated with this parameter. Increasing the case mass reduces the transducer’s output, while increasing the magnet mass slightly enhances it. Lowering the support stiffness can improve the transducer’s output and prevent chaotic motion, whereas reducing the coupler layer’s stiffness decreases the transducer’s output in otosclerosis. The results offer insights for optimizing the design of electromagnetic transducers for round-window stimulation.
Stress in the Geneva Basin
Authors: Mosar Jon , Borderie Sandra , Hauvette Louis , Marro Adeline 
Published: September 2024

Abstract

In this paper we explore the stress orientation and magnitudes of the Greater Geneva Basin in the detached Alpine Foreland in Switzerland and France. The interpretation is based on shallow earthquake focal mechanisms from strike-slip faults. The area of investigation comprises the westernmost Molasse Basin, the Vuache mountain range formed by the Vuache Fault Zone and the meridional thrusts and folds of the Haute Chaine Jura (Internal Jura). All these domains are detached along a main décollement in the Triassic Keuper Group evaporites and transported by some 30 km to the NW. The whole foreland is presently in a state of critical stress, considering the ongoing seismic activity, with hydrostatic fluid pressure conditions and an Andersonian stress setting. It is thus possible to derive locally the full stress tensor and analyze the stress state of known individual faults. The Mohr circle analysis and the distance to criticality demonstrate that the hydrostatic fluid pressure conditions allow seismic activity on optimally oriented vertical faults. Less optimally oriented faults require an additional raise in fluid pressure conditions. Differential stress values are in the range of 100-150MPa, and max horizontal stress values range up to 200MPa. Similar values are derived from 2D numerical mechanical analysis using limit analysis theory of the basin applied to a very weak décollement and steep reverse faults. In this approach different friction states were explored. The fact that not all faults are hydraulically conductive, despite favorable orientation, suggest that a valve pressure system and repeated sealing of the faults is a likely process operation in the area. This has profound consequences on fluid circulation models in geothermal systems.
Feasibility Study of Vascular Pulsatile EIT Lung Perfusion Imaging for V/Q-matched Dynamic Non-invasive Monitoring
Authors: Li Junyao , Guo Yitong , Zhu Mingxu , Wang Yu , Wang Weice , Song Ruteng , Liu Benyuan , Ji Zhenyu , Shi Xuetao 
Published: September 2024

Abstract

OBJECTIVES
Ventilation/perfusion (V/Q) matching is an important indicator for assessing lung functional status. Existing techniques for assessing V/Q in the clinic are unable to meet the demand for continuous monitoring at the bedside, and are accompanied by invasive and infected risks. Therefore, the present study is based on our team's newly developed high-performance electrical impedance tomography (EIT) system, and explores the feasibility of its use for non-invasive assessment of V/Q at the bedside by acquiring and imaging pulmonary blood flow pulsation signals and combining them with pulmonary ventilatory impedance information.
METHODS
Domestic pigs under anaesthesia (N = 8, 17.50 ± 1.51 kg) were used as experimental subjects, and continuous lung EIT imaging data acquisition was performed at 40 fps through different positions and apnoea states in pigs. By analysing the vascular pulsation impedance information, lung perfusion-related information was extracted and combined with lung ventilation impedance information to establish a vascular pulsation V/Q assessment method. In addition, the method of monitoring pulmonary perfusion by hypertonic saline (5 ml of 10% NaCl) was used as a ‘standard’ for control experiments.
RESULTS
The V/Q results obtained by both methods changed significantly with changes in body position and respiratory status. When the body position changed from supine to prone, both methods showed a decrease in V/Q values (p < 0.05). When the respiratory pause status was changed from end-expiratory pause period to end-inspiratory pause period, both methods showed an increase in V/Q values (P < 0.05). The Kappa values for both methods were 0.492, p < 0.05, for postural and 0.586, p < 0.05, for during apnoea.This demonstrated the consistency of the results of the two methods in assessing V/Q.
CONCLUSIONS
Finally, the EIT imaging based on the vascular pacing method can effectively assess V/Q changes caused by alterations during different positions and apnoea, with results consistent with the hypertonic saline method. The vascular pulsatility method is expected to be a novel bedside monitoring and assessment method to guide and improve V/Q.
Virtual Reality in the Management of Hematophobia in Hospital Settings: Impact on Anxiety Reduction
Authors: Triviño-Martínez Ángeles , Carreño-Cutillas Cristina , Azorín-Maciá Irene , Soler-Climent Esther , Carlos Sola-Verdú José , Alejandro Mira 
Published: September 2024

Abstract

Introduction: Hematophobia, a specific phobia that triggers an irrational fear of blood and related procedures such as venipuncture, can lead to the avoidance of essential medical care. Virtual reality (VR) has emerged as a promising tool to reduce anxiety associated with these procedures. This study aimed to determine the prevalence of hematophobia among patients who visit the hospital for venipuncture and to evaluate the impact of VR on reducing anxiety and fear during venipuncture, considering variables such as gender, age, number of annual hospital visits, and service.
Material and Method: An analytical and experimental study was conducted, randomized 1:1, with a sample of 185 patients of both sexes, aged between 18 and 70 years, divided into two groups: clinical analysis laboratory and hematology day hospital. Anxiety was assessed using the Visual Analog Scale (VAS), and the severity of hematophobia was measured with the Blood-Injection Phobia Inventory (BIPI).
Results: VR was more effective in reducing anxiety in patients with moderate hematophobia, with a significant reduction in pre- and post-puncture anxiety scores (p=0.012). VR was particularly effective in patients under 40 years of age (p=0.039), while no statistical significance was observed in patients over 65 years of age.
Conclusions: VR is effective in reducing anxiety during venipuncture, especially in patients with moderate hematophobia and those under 40 years of age. Its application needs to be personalized according to the clinical context.
Evaluation of Cellulolytic Gut Bacteria Isolated from White Grubs (Holotrichia serrata and Leucopholis coneophora) and Their Utilization in Lignocellulose Degradation
Authors: Vis Gatta , Prakash KV , Ramesh BN , Bhavani P , Gagandeep KR 
Published: September 2024

Abstract

Abstract
The gut microbiota of insects plays a crucial role in digesting food, providing nutrients, and synthesizing enzymes. This approach is particularly relevant for degrading lignocellulosic biomass and managing waste. In Karnataka, the larvae of Holotrichia serrata and Leucopholis canephora are major crop pests, but the role of their bacterial communities in lignocellulose degradation has not been well studied. This study aimed to isolate and evaluate bacteria from these larvae for their ability to degrade lignocellulose.Approximately seventeen cellulolytic bacterial strains were isolated from the fermentation chamber of white grubs, primarily from the Firmicutes and γ-proteobacteria classes. Notable species included Bacillus, Enterobacter, and Klebsiella. Bacillus toyonensis strain LC3B1 demonstrated significant cellulolytic activity, with a cellulolytic index of 1.93 ± 0.037. The degradation of corncob powder was the highest (28.15 ± 1.56%), followed by that of paddy straw powder (31.45 ± 0.608%) and groundnut husk powder (33.25 ± 0.823%), indicating the strong ability of these powders to degrade agricultural residues. FTIR analysis of the substrate carboxymethyl cellulose (CMC) hydrolyzed by LC3B1 revealed decomposition products such as ketones, aldehydes, alcohols, and carboxylic acids. Scanning electron microscopy (SEM) revealed significant morphological changes and the formation of pores and tunnels in the treated biomass.The diverse cellulolytic capabilities of gut bacteria from white grubs, including those of the Bacillaceae, Enterobacteriaceae, and Pseudomonadaceae families, offer promising opportunities for lignocellulosic biomass degradation, biofuel production, and sustainable waste management.
RIS-aided Massive MIMO Performance-complexity Trade-off Optimization
Authors: Abrao Taufik , Itaborahy Marco , Souza Wilson , Polvani Gabriel , Flaiban Andre , Marinello José Carlos 
Published: September 2024

Abstract

This paper explores the performance-complexity trade-off for reconfigurable intelligent surface (RIS)-aided mas- sive MIMO (mMIMO) systems, focusing on maximizing the sum spectral efficiency (SE) under Zero-Forcing (ZF) with instanta- neous channel state information (CSI). The study employs two distinct optimization approaches: manifold-based optimization and a metaheuristic evolutionary genetic algorithm (GA). The paper analyzes the effectiveness of these methods in optimizing the system sum SE under real-world passive RIS constraint. Key objectives include standardizing system models, validating the manifold-based approach and results, and evaluating the performance of both strategies. The paper highlights the pros and cons of each method and analyzes their performance for different scenarios, including various user configurations and system parameters. Numerical simulations are conducted to showcase the performance of both methods in terms of sum SE and compu- tational complexity. The study concludes by summarizing the key findings and highlighting the importance of optimizing RIS-aided mMIMO systems for enhanced communication performance and efficiency.
Shape-Recovering Liquids
Authors: Russell Thomas , Raykh Anthony , Paulsen Joseph , McGlasson Alex , Joshi Chaitanya , Atherton Timothy , Kandula Hima , Hoagland David 
Published: September 2024

Abstract

The segregation of particles to the interface between two immiscible liquids to reduce the interfacial energy is a fundamental principle that underpins emulsification and the phase behavior of a wealth of multicomponent composite systems. Breaking this textbook rule, we find that the strong binding and two-dimensional assembly of ferromagnetic particles at a liquid-liquid interface not only suppresses emulsification, but also increases the interfacial tension, as found when the interface between two immiscible liquids assumes the shape of a Grecian urn. This shape is rapidly and reproducibly recovered after being destroyed by vigorous agitation. The suppression of emulsification, the rapid formation of a stable, non-planar equilibrium shape of the interface, and the increase in interfacial tension have their origins in the attractive in-plane dipolar magnetic interactions between the particles.
Molecular bases of the interactions of ATG16L1 with FIP200 and ATG8 family proteins
Authors: Pan Lifeng , Gong Xinyu , Zhou Yuqian , Wang Yingli , Tang Yubin , Liu Haobo , Zhou Xindi , Zhang Yuchao , Guo Hanbo , Guo Zhenpeng 
Published: September 2024

Abstract

Macroautophagy maintains cellular and organismal homeostasis, and entails de novo synthesis of double-membrane autophagosome. The effective formation of autophagosome requires the recruitment of the ATG12~ATG5-ATG16L1complex to the pre-autophagosomal structure by relevant ATG16L1-binding autophagic factors including FIP200. However, the molecular mechanism governing the specific interaction of ATG16L1 with FIP200 remains elusive. Here, we uncover that ATG16L1 contains a FIP200-interacting region (FIR), which not only can directly bind to the Claw domain of FIP200, but also can serve as an atypical ATG8-interacting motif (AIM) to selectively recognize mammalian ATG8 family proteins. We determine the high-resolution crystal structures of ATG16L1 FIR in complex with FIP200 Claw and GABARAPL1, respectively, and elucidate the molecular mechanism underlying the interactions of ATG16L1 with FIP200 and ATG8 family proteins. To distinguish the precise contribution of FIP200 from ATG8 family proteins for binding to ATG16L1 FIR in autophagy, we develop a unique ATG16L1 mutant that can exclusively interact with ATG8 family proteins but not FIP200. Finally, using relevant cell-based functional assays, we demonstrate that the interaction of ATG16L1 with FIP200 is indispensable for the effective autophagic flux. In conclusion, our findings provide mechanistic insights into the interactions of ATG16L1 with FIP200 and ATG8 family proteins, and are valuable for further understanding the function of ATG16L1 in autophagy.
Influence of Reestablishing Greater Tuberosity Angle on Patient Outcomes Following Greater Tuberosity Fractures
Authors: Castanheira Andre , Amaro Pedro , Alonso Raul , Pires Luis 
Published: September 2024

Abstract

Purpose: This study aimed to assess whether values of GTA after osteosynthesis of isolated greater tuberosity fractures were associated with worse functional and symptomatic outcomes.
Methods: A retrospective analysis was conducted from 2012 till 2024, including all patients with isolated greater tuberosity fractures who underwent osteosynthesis at a single institution. GTA measurements before and after osteosynthesis were recorded, along with the latest quick Dash score and CONSTANT shoulder score
Results: Thirty-four patients were recruited, with 25 undergoing osteosynthesis with cannulated screws and 8 with anchors. Twenty-two patients self-administered the QuickDash score and completed the Constant–Shoulder score. The average GTA before surgery was 84.2°, which was reduced to 62.2° postsurgery. The average CONSTANT score was 86.8, and the mean Quick Dash score was 7.65. The method of osteosynthesis did not significantly influence the GTA or the QuickDash or Constant scores. Additionally, postsurgery GTA was negatively correlated with the CONSTANT score(r=-0.65, p<0.01).
Conclusions: Patients with isolated greater tuberosity fractures who underwent osteosynthesis experienced a change in the GTA after surgery. However, they achieved satisfactory symptomatic and functional scores postsurgery. The method of osteosynthesis did not significantly influence the GTA or the QuickDash or CONSTANT scores. Furthermore, postsurgery GTA was negatively correlated with the CONSTANT score.