bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cholinergic Stimulation Improves Oxidative Stress and Inflammation in Experimental Myocardial Infarction
Authors: Bezerra Otávio C. , França Cristiane Miranda , Rocha Juraci Aparecida , Neves Gizele A. , Souza Pamella Ramona M. , Teixeira Gomes Mariana , Malfitano Christiane , Loleiro Tatiane C. Alba , Dourado Paulo Magno , Llesuy Susana , de Angelis Katia , Irigoyen Maria Claudia C. , Ulloa Luis , Consolim-Colombo Fernanda M. 
Published: October 2017

AbstractWe previously reported that cholinergic stimulation with pyridostigmine (PY) induces anti-inflammatory cell recruitment soon after myocardial infarction (MI). In this study, we evaluated the anti-inflammatory effects of PY during the proliferative phase of cardiac repair by analyzing the infiltration of macrophages, Treg lymphocytes, oxidative stress and inflammatory cytokines. Wistar rats underwent control sham surgery or ligation of the left coronary artery and were randomly allocated to remain untreated (untreated infarcted group, I) or to receive PY (30 mg·kg(−1)·day(−1)) in the supplied water (infarcted treated group, I + PY). Blood pressure and heart rate variability were registered at day 5 post-MI. The animals were euthanized 7 days after thoracotomy, when the hearts were removed and processed for immunohistochemistry (CD68, CD206, FOXP3), cytokines (IL-1β, IL-6, IL-10, TNF-α) and oxidative stress (superoxide dismutase, catalase, glutathione peroxidase, lipidic and protein peroxidation). PY treatment increased parasympathetic modulation, M2 macrophages and the anti-oxidant enzyme activity but reduced protein oxidation (carbonyls) and the concentration of IL-1β, IL-6, TNF-α and IL-10. Cholinergic stimulation induces parasympathetic neuro-immune modulation and anti-inflammatory cell enrollment as well as prevents oxidative stress and cytokine production after MI.
Helicobacter pylori γ-glutamyl transferase contributes to colonization and differential recruitment of T cells during persistence
Authors: Wüstner Stefanie , Anderl Florian , Wanisch Andreas , Sachs Corinna , Steiger Katja , Nerlich Andreas , Vieth Michael , Mejías-Luque Raquel , Gerhard Markus 
Published: October 2017

AbstractHelicobacter pylori γ-glutamyl transferase (gGT) is a key bacterial virulence factor that is not only important for bacterial gastric colonization but also related to the development of gastric pathology. Despite accumulating evidence for pathogenic and immunologic functions of H. pylori gGT, it is still unclear how it supports gastric colonization and how its specific effects on the host’s innate and adaptive immune responses contribute to colonization and pathology. We have compared mice showing similar bacterial load after infection with gGT-proficient or gGT-deficient H. pylori to analyse the specific role of the enzyme during infection. Our data indicate that H. pylori gGT supports initial colonization. Nevertheless, bacteria lacking gGT can still colonize and persist. We observed that the presence of gGT during infection favoured a proinflammatory innate and adaptive immune response. Notably, H. pylori gGT activity was linked to increased levels of IFNγ, which were attributed to a differential recruitment of CD8+ T cells to the stomach. Our data support an essential role for H. pylori gGT in gastric colonization and further suggest that gGT favours infiltration of CD8+ cells to the gastric mucosa, which might play an important and yet overlooked role in the pathogenesis of H. pylori.
Molecular spectrum of excision repair cross-complementation group 8 gene defects in Chinese patients with Cockayne syndrome type A
Authors: Wang Xiaozhu , Huang Yu , Yan Ming , Li Jiuwei , Ding Changhong , Jin Hong , Fang Fang , Yang Yanling , Wu Baiyan , Chen Dafang 
Published: October 2017

AbstractThere are two genetics complementary groups Cockayne syndrome type A and B (CS-A and CS-B OMIM 216400, 133540), which is a rare autosomal recessive segmental progeroid syndrome. Homozygous or compound heterozygous mutations in the excision repair cross-complementation group 8 gene (ERCC8) result in CS-A, and mutations in ERCC6 result in CS-B. Homozygous ERCC6/ERCC8 mutations also result in UV-sensitive syndrome. In this study, twenty-one Han Chinese patients with CS were investigated to identify mutations in ERCC8/ERCC6, of which thirteen cases with CS-A were identified with the mutations of ERCC8. There are five types mutations of ERCC8 in our study, such as exon 4 rearrangement, c.394_398delTTACA, c.299insA, c.843 + 2 T > C, and c.2 T > A. An estimated frequency of exon 4 rearrangement accounts for 69.23% and c.394_398delTTACA accounts for 11.53% in our cohort. Haplotype analysis revealed that the exon 4 rearrangement and c.394_398delTTACA mutations originated from a common founder in the Chinese population respectively. With the identification of three novel ERCC8 mutations, this study expanded the molecular spectrum of known ERCC8 defects, and furthermore, suggests that the exon 4 rearrangement and c.394_398delTTACA mutations may be a common underlying cause of CS-A in the Chinese population, which is different from that in other populations.
MultiFLEXX - The new multi-analyzer at the cold triple-axis spectrometer FLEXX
Authors: Groitl Felix , Toft-Petersen Rasmus , Quintero-Castro Diana Lucia , Meng Siqin , Lu Zhilun , Huesges Zita , Le Manh Duc , Alimov Svyatoslav , Wilpert Thomas , Kiefer Klaus , Gerischer Sebastian , Bertin Alexandre , Habicht Klaus 
Published: October 2017

AbstractThe first experimental characterization of a multiple energy analysis wide angle backend for a cold triple-axis spectrometer is reported. The multi-analyzer module MultiFLEXX employs 155 detection channels which simultaneously probe an extensive range in wavevector and energy transfer. Successful mapping of magnetic excitations in MnF2and Ho demonstrate order of magnitude gains in data collection efficiency using this novel type backend. MultiFLEXX is competitive to standard triple-axis spectroscopy in terms of energy resolution and signal-to-noise ratio. A minority of the detector channels is affected by spurious signals inherent to this multiplexing concept. The characteristic signature of these spurious signals easily allows for their discrimination. The instrument concept focuses on detection efficiency in the horizontal scattering plane which makes it an ideal technique for fast mapping and parametric studies including extreme sample environment.
Analyzing homoeolog expression provides insights into the rediploidization event in gynogenetic hybrids of Carassius auratus red var. × Cyprinus carpio
Authors: Ren Li , Cui Jialin , Wang Jing , Tan Hui , Li Wuhui , Tang Chenchen , Qin Qinbo , Liu Shaojun 
Published: October 2017

AbstractRediploidization is considered to be a part of the evolutionary history of allotetraploids, and resulted in the emergence of novel epigenetic regulatory activities. To study the changing patterns of gene expression following the reduction of a genome by 50%, we used RNA-seq and quantitative real-time PCR (qPCR) to investigate total gene expression and homoeolog expression in three hybrids of a C. auratus red var. (2n = 100, ♀) (R) and C. carpio (2n = 100, ♂) (C) (i.e., F1, F18, and G4) and their original parents. A comparison of homoeolog expression between G4 and F18 identified 7 genes (0.22%) that exhibited novel R/C homoeolog expression patterns in G4, while 4 genes (0.12%) were affected by R/C homoeolog silencing. We determined the direction and extent of the homoeolog expression bias (HEB). The C-HEB genes (i.e., nrp1a and igf1) and R-HEB genes (i.e., fgf23 and esm1) provided insights into the effects of the dominance of one parental homoeolog expression on growth regulation. This dominance may contribute to the rapid growth of G4 fish. Our findings may be relevant for clarifying the relationship between growth heterosis and differences in homoeolog expression patterns.
Significance of CCL2, CCL5 and CCR2 polymorphisms for adverse prognosis of Japanese encephalitis from an endemic population of India
Authors: Chowdhury Purvita , Khan Siraj Ahmed 
Published: October 2017

AbstractJapanese encephalitis (JE) is a major contributor for viral encephalitis in Asia. Vaccination programme has limited success for largely populated JE endemic countries like India and disease exposure is unavoidable. Involvement of chemokines and its co-receptors for adverse prognosis of JE have been documented both in vitro and in vivo. Identification of the genetic predisposing factor for JE infection in humans is crucial but not yet established. Therefore, we investigated the association of single nucleotide polymorphisms (SNPs) in chemokines (CCL2 and CCL5) and its co-receptors (CCR2 and CCR5) with their protein level for JE. The study enrolled 87 symptomatic JE cases (mild: severe = 24:63) and 94 asymptomatic controls. Our study demonstrated that CCL2 (rs1024611G), CCL5 (rs2280788G) and CCR2 (rs1799864A) significantly associated with JE (Odds ratio = 1.63, 2.95 and 2.62, respectively and P = 0.045, P = 0.05 and P = 0.0006, respectively). The study revealed that rs1024611G allele was associated with elevated level of CCL2. CCL5 elevation associated with JE mortality having a Cox proportional hazard of 1.004 (P = 0.033). In conclusion, SNPs of chemokine viz. CCL2 (rs1024611G) and its receptor CCR2 (rs1799864A) significantly associated with JE which may serve as possible genetic predisposing factor and CCL5 protein level may act as marker for disease survival.
The rice TRIANGULAR HULL1 protein acts as a transcriptional repressor in regulating lateral development of spikelet
Authors: Peng Peng , Liu Lihua , Fang Jingjing , Zhao Jinfeng , Yuan Shoujiang , Li Xueyong 
Published: October 2017

AbstractAs a basic unit of rice inflorescence, spikelet has profound influence on grain size, weight and yield. The molecular mechanism underlying spikelet development has not been fully elucidated. Here, we identified four allelic rice mutants, s2-89, xd151, xd281 and xd425, which exhibited reduced width of spikelet, especially in the apical region. Map-based cloning revealed that all these mutants had missense mutation in the TRIANGULAR HULL1 (TH1) gene, encoding an ALOG family protein. TH1 has been shown to regulate the lateral development of spikelet, but its mode of action remains unclear. Microscopic analysis revealed that the reduction in spikelet width was caused by decreased cell size rather than cell division. The TH1 protein was shown to localize in the nucleus and possess transcriptional repression activity. TH1 could form a homodimer and point mutation in the s2-89, xd281 and xd425 mutant inhibited homodimerization. The transcriptional repression activity of TH1 was partially relieved by the His129Tyr substitution in the s2-89 mutant. Fusion of an exogenous EAR transcription suppression domain to the mutant protein TH1s2-89 could largely complemented the narrow spikelet phenotype. These results indicate that TH1 functions as a transcription repressor and regulates cell expansion during the lateral development of spikelet.
Posttranslational modifications of blood-derived alpha-synuclein as biochemical markers for Parkinson’s disease
Authors: Vicente Miranda Hugo , Cássio Rafaela , Correia-Guedes Leonor , Gomes Marcos António , Chegão Ana , Miranda Elisa , Soares Tiago , Coelho Miguel , Rosa Mário Miguel , Ferreira Joaquim J. , Outeiro Tiago Fleming 
Published: October 2017

AbstractParkinson’s disease (PD) is a progressive neurodegenerative disorder known for the typical motor features associated. Pathologically, it is characterized by the intracellular accumulation of alpha-synuclein (aSyn) in Lewy bodies and Lewy neurites. Currently, there are no established biochemical markers for diagnosing or for following disease progression, a major limitation for the clinical practice. Posttranslational modifications (PTMs) in aSyn have been identified and implicated on its pathobiology. Since aSyn is abundant in blood erythrocytes, we aimed to evaluate whether PTMs of aSyn in the blood might hold value as a biomarker for PD. We examined 58 patients with PD and 30 healthy age-matched individuals. We found that the levels of Y125 phosphorylated, Y39 nitrated, and glycated aSyn were increased in PD, while those of SUMO were reduced. A combinatory analysis of the levels of these PTMs resulted in an increased sensitivity, with an area under curve (AUC) of 0.843 for PD versus healthy controls, and correlated with disease severity and duration. We conclude that the levels of these selected PTMs hold strong potential as biochemical markers for PD. Ultimately, our findings might facilitate the monitoring of disease progression in clinical trials, opening the possibility for developing more effective therapies against PD.
Antibiotics ameliorate lupus-like symptoms in mice
Authors: Mu Qinghui , Tavella Vincent J. , Kirby Jay L. , Cecere Thomas E. , Chung Matthias , Lee Jiyoung , Li Song , Ahmed S. Ansar , Eden Kristin , Allen Irving Coy , Reilly Christopher M. , Luo Xin M. 
Published: October 2017

AbstractGut microbiota and the immune system interact to maintain tissue homeostasis, but whether this interaction is involved in the pathogenesis of systemic lupus erythematosus (SLE) is unclear. Here we report that oral antibiotics given during active disease removed harmful bacteria from the gut microbiota and attenuated SLE-like disease in lupus-prone mice. Using MRL/lpr mice, we showed that antibiotics given after disease onset ameliorated systemic autoimmunity and kidney histopathology. They decreased IL-17-producing cells and increased the level of circulating IL-10. In addition, antibiotics removedLachnospiraceaeand increased the relative abundance ofLactobacillusspp., two groups of bacteria previously shown to be associated with deteriorated or improved symptoms in MRL/lpr mice, respectively. Moreover, we showed that the attenuated disease phenotype could be recapitulated with a single antibiotic vancomycin, which reshaped the gut microbiota and changed microbial functional pathways in a time-dependent manner. Furthermore, vancomycin treatment increased the barrier function of the intestinal epithelium, thus preventing the translocation of lipopolysaccharide, a cell wall component of Gram-negativeProteobacteriaand known inducer of lupus in mice, into the circulation. These results suggest that mixed antibiotics or a single antibiotic vancomycin ameliorate SLE-like disease in MRL/lpr mice by changing the composition of gut microbiota.
Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs
Authors: Han Shengli , Lv Yanni , Kong Liyun , Che Delu , Liu Rui , Fu Jia , Cao Jiao , Wang Jue , Wang Cheng , He Huaizhen , Zhang Tao , Dong Xinzhong , He Langchong 
Published: October 2017

AbstractAnaphylactoid reactions are common clinical acute adverse drug reactions that can exacerbate a patient’s condition and produce effects that may become life-threatening. Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a sensitive and rapid method to detect histamine release from LAD2 cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and constructed a relative release index based on various release curve parameters, including allergen release time and sudden change rate, to evaluate the potential and strength of allergen-induced anaphylactoid reactions. This LAD2 release model was used to evaluate anaphylactoid reactions induced by ciprofloxacin, norfloxacin, lomefloxacin, moxifloxacin, and baicalin. The results positively correlated with those obtained with an Evans blue ear test and negatively correlated with the Ca2+ influx EC50. In summary, the current study established a novel in vitro method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions.
Investigation of foaming causes in three mesophilic food waste digesters: reactor performance and microbial analysis
Authors: He Qin , Li Lei , Zhao Xiaofei , Qu Li , Wu Di , Peng Xuya 
Published: October 2017

AbstractFoaming negatively affects anaerobic digestion of food waste (FW). To identify the causes of foaming, reactor performance and microbial community dynamics were investigated in three mesophilic digesters treating FW. The digesters were operated under different modes, and foaming was induced with several methods. Proliferation of specific bacteria and accumulation of surface active materials may be the main causes of foaming. Volatile fatty acids (VFAs) and total ammonia nitrogen (TAN) accumulated in these reactors before foaming, which may have contributed to foam formation by decreasing the surface tension of sludge and increasing foam stability. The relative abundance of acid-producing bacteria (Petrimonas, Fastidiosipila, etc.) and ammonia producers (Proteiniphilum, Gelria, Aminobacterium, etc.) significantly increased after foaming, which explained the rapid accumulation of VFAs and NH4+ after foaming. In addition, the proportions of microbial genera known to contribute to foam formation and stabilization significantly increased in foaming samples, including bacteria containing mycolic acid in cell walls (Actinomyces, Corynebacterium, etc.) and those capable of producing biosurfactants (Corynebacterium, Lactobacillus, 060F05-B-SD-P93, etc.). These findings improve the understanding of foaming mechanisms in FW digesters and provide a theoretical basis for further research on effective suppression and early warning of foaming.
A detailed X-ray investigation of ζ Puppis
Authors: Nazé Yaël , Ramiaramanantsoa Tahina , Stevens Ian R. , Howarth Ian D. , Moffat Anthony F. J. 
Published: October 2017

Context. One of the optically brightest and closest massive stars, ζ Pup, is also a bright X-ray source. Previously, its X-ray emission was found to be variable with light curves harbouring “trends” with a typical timescale longer than the exposure length, i.e. >1 d. The origin of these changes was proposed to be linked to large-scale structures in the wind of ζ Pup, but further characterization of the variability at high energies was needed to investigate this scenario.
Aims. Since the previous papers of this series, a number of new X-ray observations have become available. Furthermore, a cyclic behaviour with a 1.78 d period was identified in long optical photometric runs, which is thought to be associated with the launching mechanism of large-scale wind structures.
Methods. We analysed these new X-ray data, revisited the old data, and compared the X-ray light curves with the optical data, notably those taken simultaneously.
Results. The behaviour of ζ Pup in X-rays cannot be explained in terms of a perfect clock because the amplitude and shape of its variations change with time. For example, ζ Pup was much more strongly variable between 2007 and 2011 than before and after this interval. Comparing the X-ray spectra of the star at maximum and minimum brightness yields no compelling difference beyond the overall flux change: the temperatures, absorptions, and line shapes seem to remain constant, well within errors. The only common feature between X-ray datasets is that the variation amplitudes appear maximum in the medium (0.6–1.2 keV) energy band. Finally, no clear and coherent correlation can be found between simultaneous X-ray and optical data. Only a subgroup of observations may be combined coherently with the optical period of 1.78 d, although the simultaneous optical behaviour is unknown.
Conclusions. The currently available data do not reveal any obvious, permanent, and direct correlation between X-ray and optical variations. The origin of the X-ray variability therefore still needs to be ascertained, highlighting the need for long-term monitoring in multiwavelengths, i.e. X-ray, UV, and optical.
The Effects of Aspirin in Gestation and Reproduction (EAGeR) Trial: A Story of Discovery
Authors: Connell Matthew , Sjaarda Lindsey , Radin Rose , Kuhr Daniel , Mumford Sunni , Plowden Torie , Silver Robert , Schisterman Enrique 
Published: October 2017

AbstractHuman reproduction is an inefficient process. There are several drivers of complications along the path to and during pregnancy, one of which is inflammation. Treatments to mitigate the deleterious effects of aberrant inflammation with something inexpensive and widely available like aspirin could have dramatic global impact. The Effects of Aspirin in Gestation and Reproduction (EAGeR) trial enrolled women aged 18 to 40 years with one to two prior pregnancy losses and no diagnosis of infertility. Patients were randomized to either low-dose aspirin or placebo. Here, we review the collective findings of the EAGeR trial to date and discuss several important lessons learned from the unique data resulting from this groundbreaking trial. Findings reported from this trial provide significant advances in the understanding of aspirin's potential mechanisms in modulating reproductive processes and the role of inflammation in these processes. This review describes the collective findings of the EAGeR trial in the context of the existing literature regarding aspirin and inflammation in reproduction to inform relevant next steps in fertility and obstetric research, as well as potential implications for clinical care.
Pulmonary Hypertension in Patients with Congenital Diaphragmatic Hernia: Does Lung Size Matter?
Authors: Madenci Arin , Gajarski Robert , Marchetti Kathryn , Klein Edwin , Coughlin Megan , Kreutzmann Jeannie , Treadwell Marjorie , Ladino-Torres Maria , Mychaliska George , Church Joseph 
Published: October 2017

Purpose The relationship between pulmonary hypoplasia and pulmonary arterial hypertension (PHTN) in patients with congenital diaphragmatic hernia (CDH) remains ill-defined. We hypothesized that prenatal estimates of lung size would directly correlate with PHTN severity.
Methods Infants with isolated CDH (born 2004–2015) at a single institution were included. Estimates of lung size included observed-to-expected LHR (o:eLHR) and %-predicted lung volumes (PPLV = observed/predicted volumes). The primary outcome was severity of PHTN (grade 0–3) on echocardiography performed between day of life 3 and 30.
Results Among 62 patients included, there was 32% mortality and 65% ECMO utilization. PPLV (odds ratio [OR] = 0.94 per 1 grade in PHTN severity, 95% confidence interval [CI] = 0.89–0.98, p < 0.01) and o:eLHR (OR = 0.97, 95% CI = 0.94–0.99, p < 0.01) were significantly associated with PHTN grade. Among patients on ECMO, PPLV (OR = 0.92, 95% CI = 0.84–0.99, p = 0.03) and o:eLHR (OR = 0.95, 95% CI = 0.92–0.99, p = 0.01) were more strongly associated with PHTN grade. PPLV and o:eLHR were significantly associated with the use of inhaled nitric oxide (iNO) (OR = 0.90, 95% CI = 0.83–0.98, p = 0.01 and OR = 0.94, 95% CI = 0.91–0.98, p < 0.01, respectively) and epoprostenol (OR = 0.91, 95% CI = 0.84–0.99, p = 0.02 and OR = 0.93, 95% CI = 0.89–0.98, p < 0.01, respectively).
Conclusion Among infants with isolated CDH, PPLV, and o:eLHR were significantly associated with PHTN severity, especially among patients requiring ECMO. Prenatal lung size may help predict postnatal PHTN and associated therapies.