bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular β-Glucosidase Production from bglp15.2 Gene Carrying Inulinase Signal Peptide in Saccharomyces cerevisiae BY4741
Authors: Fitri Armaya Badiatul , Restiawaty Elvi , Moeis Maelita Ramdani 
Published: November 2017

One of the important enzymes in cellulase complex is β-glucosidase. In this research, adding signal peptide of inulinase gene from Kluyveromyces marxianus, cloning, and expressing of bglp15.2 gene in S. cerevisiae BY4741 had been done. Gene of bglp15.2 encoding β-glucosidase has 90% identity to nucleotide sequence of Shewanella frigidimarina NCIMB 400 bacteria. Adding nucleotide sequence of signal peptide was aimed to secrete β-glucosidase and had been done with PCR (Polymerase Chain Reaction) method. The addition of nucleotide sequence of signal peptide in bglp15.2 gene had been done succesfully that indicated from nucleotide sequencing result and the increment of amplicon band size in electroferogram of the last addition PCR step. The bglp15.2 and bglp15.2INU gene (the bglp15.2 gene that has signal peptide nucleotide sequence) were cloned in Escherichia coli DH5α using pGEM-T-Easy vector and pBEVY-GL shuttle vector. The pBEVY-GL shuttle vector was used for transforming S. cerevisiae BY4741 with bglp15.2 and bglp15.2INU. The recombinant S. cerevisiae BY4741 carrying bglp15.2INU gene and growing in 48 hours had extracellularly β-glucosidase enzyme activity of 0,0178 U/ml and the intracellularly activity was 0,0181 U/ml. The β-glucosidase enzyme without signal peptide was not secreted. With K. marxianus inulinase signal peptide, about 50% Bglp15.2INU protein could be secreted. The protein molecular weight of secreted Bglp15.2INU was 44 kDa in SDS-PAGE result.
Omega-3 Fatty Acid Effect in Cardiovascular Disease Risk Factors
Authors: Mohammed Alketbi Fatima , Lilian Bolaji , Antonis Zampelas 
Published: November 2017

Background: One of the leading causes of death around the globe is cardiovascular disease (CVD). Nutrition plays a vital role in the prevention of CVD. However, the effects of margarines, rich in n-3 and n-6 fatty acids, compared to butter, are still controversial in plasma lipid levels. Method: A single blind parallel study was carried out. Twelve (n=12) healthy UAE University female students were recruited from age 19-24 years old and were randomly allocated in 2 groups. The participants were asked to consume either 25g of margarine or 25g of butter for 1 month and to retain their normal daily dietary habits. Fasting blood samples were collected before the beginning and at the end of the dietary periods for the assessment of lipid profile.Result: Triglycerides decreased by 12% within the intervention group, total cholesterol, and glucose levels were decreased in both groups, but it was not statistically significant within and between the margarine and butter group.Conclusion: The present study showed no statistical differences in the effects of butter and margarine, enriched with n-3 and n-6 fatty acid, on cholesterol, triglycerides, and glucose. Further research is necessary with bigger sample size and longer duration.
Caffeine Consumption among Zayed University Students in Dubai, United Arab Emirates: A Cross-Sectional Study
Authors: M. Al Ghali Rola , Al Shaibi Hessa , Al Majed Huda , Haroun Dalia 
Published: November 2017

Background: Over the past decade, the global caffeine consumption rate has increased dramatically. Coffee and tea are among the common sources, but energy drinks are becoming an important contributor to total caffeine consumption. Recommendations for daily caffeine intake is not being followed due to the perceived benefits of caffeine which include mood improvement, concentration, social factors, and energy boosting. This study was important to add to the limited data about caffeine consumption in the Gulf region and mainly in the UAE.Objectives: The objectives were to determine the prevalence of caffeinated beverage consumption among university students and perceived benefits in addition to the estimation of daily caffeine consumption (mg/day).Design: Data was collected through a self-reported questionnaire from a total of 175 participants (129 females and 46 males) who were conveniently selected from different settings at Zayed University - Dubai. Usual Caffeine intake was calculated from all caffeine containing beverages. Results: Eighty-six percent of the 175 participants, both males and females, at Zayed University-Dubai consumed caffeinated beverages with an average intake of 249.7±235.9 mg. The intake among the 150 caffeine consumers varied from 4.2 mg/day to 932.2 mg/day. Average intake of caffeine was not significantly different between genders (P=0.125). Thirty-five percent of the population consumed more than 400 mg/day of caffeine, with no statistical difference between males and females (P=0.202). Coffee was the most commonly consumed drink among both genders, followed by tea. Most perceived benefits of caffeine consumption were not significantly different except for the relationship between caffeine and improved exercise performance (P=0.018) and caffeine in relation to weight loss (P=0.001) among males.Conclusion: The prevalence of caffeine consumption at Zayed University was high among both males and females. Further research is necessary to estimate the total caffeine intake from all dietary sources, mainly the cultural foods and beverages, and to determine the relationship between accessibility to caffeine containing beverages and caffeine consumption among University student.
Assessment of Body Composition, Endurance and Nutrient Intakes among Females Team Players in Sports Club
Authors: Attlee Amita , S. Altunaiji Shaimaa , Muayyad Mariam , Ali Zainab , Hashim Mona , Obaid Reyad S. 
Published: November 2017

Aim: The aim of the study was to assess the body composition, endurance level and usual nutrient intakes in female players representing a Sports Club in Sharjah, United Arab Emirates.Materials and Methods: Twenty-six adult female players aged between 15-24 years were selected from three different teams (basketball=12, tennis=4, volleyball=10) using convenience sampling technique. All participants were assessed for body composition through bioelectrical impedance method, endurance level using step test and nutrient intakes using 24-hour recall method. Significant differences (P < 0.05) were determined among the three teams in relation to body composition, endurance levels and nutrient intakes.Results: Body composition of players in three sports was significantly different in terms of body mass index, body fat mass, and percentage body fat and fitness scores. Tennis players had significantly higher body fat mass (28.5 ± 8.2 kg) and percent body fat (41 ± 7%) in contrast to that in basketball players (body fat mass: 19.2 ± 10.5 kg; percent body fat: 30.6 + 7.9%) and tennis players (body fat mass: 13 ± 4.2 kg; percent body fat: 26.5 ± 6.5%), respectively. On the other hand, volleyball players had significantly higher fitness score (72.2 ± 3.5) as compared to basketball players (71 ± 6.7), and tennis players (63 ± 8.2).On an average, volleyball players scored “very good” endurance level in contrast to “good” scores in basketball and tennis team players. However, this difference was not statistically significant.The average intakes of all nutrients including energy, protein, vitamins and minerals were below the recommended intakes among players of all sports teams. Conclusions: Body composition and endurance level differ with the type of sports. Volleyball team players had the lowest BMI, body fat mass as well as percent body fat and highest fitness score and endurance level. However, the overall nutrient intakes of the female players representing the three teams were less than the recommended allowances for highly active women and did not differ with the type of sports played.
Impact of Age and Sex on Bystander Cardiopulmonary Resuscitation Knowledge
Authors: Olutoyin Onyeaso Adedamola , Oluferanmi Onyeaso Onyeadikachi 
Published: November 2017

Abstract
Objective: To assess the impact of age and sex on the bystander CPR Knowledge in a group of Nigerian student teachers.
Materials and Methods: A quasi-experimental study design was used with the cohort made up of forty one (41) male and forty one (41) female undergraduate students of Physical and Health Education with 40 participants in the 17-20 years age group and 42 participants belonging to the 21-28 years of age bracket were randomly selected from the larger main cohort for analysis of possible impact of age and sex on their pre-training and post-training cardiopulmonary resuscitation knowledge. In addition to the descriptive statistics, one-way analysis of variance (ANOVA) was used to test the null hypotheses generated with significance level set at P < 0.05.
Results: No significant age impact was found on the pre-training and post-training CPR knowledge of the student teachers (P > 0.05). However, there was a positive impact of male gender on post-training CPR knowledge of the participants (P <0.001).
Conclusion: Although age could not impact significantly on the cardiopulmonary resuscitation knowledge of the participants, male gender significantly impacted on the post-training cardiopulmonary resuscitation knowledge of the cohort.
English Professors’ Attitudes towards the Use of ICT in Moroccan Universities
Authors: Laabidi Hicham 
Published: November 2017

<em>Computers offer several opportunities that encourage the promotion of teaching and learning experiences. The implementation of computer technologies in education has become the main goal of many educational reforms throughout the globe. The Moroccan kingdom, like many other nations, has understood the importance of using computers in schools and universities. For this reason, the Moroccan government has launched numerous projects aiming at the diffusion of Information and Communication Technologies (ICT) in education system. This paper examined the attitudes of professors of English toward the use of computers in teaching practices. The Theory of Diffusion of Innovation ( Rogers, 1995) served as the theoretical framework. A survey questionnaire, “Computer Attitude Scale” (CAS), was distributed to the EFL professors during the university year 2015-2016. The CAS that was developed by Loyd and Gressard ( 1984) was utilized to collect data regarding professors’ attitudes. These attitudes were assessed based on four components: anxiety, confidence, liking and usefulness. Descriptive analysis of percentages, means, and standard deviations were used to analyse the collected data. The study revealed that Moroccan professors of English language posses positive attitudes toward the implementation of computer technologies in instructional practices.</em>
Improving forecasting accuracy of river flow using gene expression programming based on wavelet decomposition and de-noising
Authors: Lu Xiaorong , Wang Xuelei , Zhang Liang , Zhang Ting , Yang Chao , Song XinXin , Yang Qing 
Published: November 2017

Abstract
Due to the effects of anthropogenic activities and natural climate change, streamflows of rivers have gradually decreased. In order to maintain reliable water supplies, reservoir operation and water resource management, accurate streamflow forecasts are very important. Based on monthly flow data from five hydrological stations in the middle and lower parts of the Hanjiang River Basin, between 1989 and 2009, we consider an efficient approach of adopting the gene expression programming model based on wavelet decomposition and de-noising (WDDGEP) to forecast river flow. Original flow time series data are initially decomposed into one sub-signal approximation and seven sub-signal details using the dmey wavelet. A wavelet threshold de-noising method is also applied in this study. Data that have been de-noised after decomposition are then adopted as inputs for WDDGEP models. Finally, the forecasted sub-signal results are summed to formulate an ensemble forecast for the original monthly flow series. A comparison of the prediction accuracy between the two models is based on three performance evaluation measures. Results show that the new WDDGEP models can effectively enhance accuracy in forecasting streamflow, and the proposed wavelet-based de-noising of the observed non-stationary time series is an effective measure to improve simulation accuracy.
Does Postoperative Mechanical Axis Alignment Have an Effect on Clinical Outcome of Primary Total Knee Arthroplasty? A Retrospective Cohort Study
Authors: Salzmann Mikhail , Fennema Peter , Becker Roland , Hommel Hagen 
Published: November 2017

Background:
There is an ongoing debate whether patients with constitutional varus should be restored to neutral mechanical alignment following total knee arthroplasty (TKA).

Objective:
The aim of this retrospective cohort study is to determine whether mild unintentional postoperative varus alignment (3°–6°) influences TKA outcome in patients with and without preoperative varus alignment due to medial osteoarthritis of the knee.

Methods:
We analyzed 172 consecutive TKA cases between April 2011 and May 2014. Patients were divided into four groups based on their preoperative and postoperative hip-knee-ankle angles (HKA): preoperative varus ≤ 3° with postoperative varus position ≤ 3° (Group 1, n = 47); preoperative varus >3° with postoperative varus ≤ 3° (Group 2, n = 104); preoperative varus ≤ 3° with postoperative varus malalignment > 3° (Group 3, n = 3); and preoperative varus > 3° with postoperative varus malalignment > 3° (Group 4, n = 18). Patients were followed up until 2 years postoperatively.

Results:
Knee Society Score and Western Ontario and McMaster University Osteoarthritis Index scores for all study groups increased following TKA, with no postoperative differences at any time point. Group 4 performed significantly better on the Forgotten Joint Score than Group 2 (p = 0.019). Group 4 performed significantly better on the High Flexion Knee Score than Group 2 (p = 0.004) and Group 1 (p = 0.019). All other between-group differences were not statistically significant.

Conclusion:
Residual postoperative varus alignment of the lower limb does not appear to adversely affect clinical outcome following TKA for varus-type osteoarthritis.
Effect of Femoral Stem Modular Neck’s Material on Metal Ion Release
Authors: Barry Janie , Kiss Marc-Olivier , Massé Vincent , Lavigne Martin , Matta Jihad , Vendittoli Pascal-Andre 
Published: November 2017

Background:
In recent decades, the popularity of modular necks in total hip arthroplasty (THA) has increased since modular necks offer the potential to restore the patient’s native anatomy, and thus improve stability. Unfortunately, modular necks are associated with higher complication rates, including implant fracture and modular junction corrosion with adverse local tissue reaction to metal debris.

Objective:
The objective of this study was to determine the impact of modular neck material on titanium (Ti), chrome (Cr), and cobalt (Co) metal ion levels in patients who underwent a THA with Ti femoral stem, Ti or CrCo modular neck, and ceramic-on-ceramic (CoC) bearing.

Methods:
Whole blood Ti, Cr, and Co levels were compared at a minimum 1-year follow-up in 36 patients who underwent unilateral, primary CoC large-diameter head THA with Profemur® Preserve modular femoral stems (MicroPort, Arlington, TX, USA).

Results:
Higher Co concentrations were observed in the CrCo modular neck group (0.46 versus 0.26 µg/l in the Ti neck group, P=0.004) and higher Ti concentrations were observed in the Ti modular neck group (1.98 vs 1.59 µg/l in the CrCo neck group, P=0.007). However, these differences were not clinically meaningful since the absolute values remained within what is considered the safe range of Ti, Cr, and Co ions in whole blood. No patients were re-operated or revised.

Conclusion:
Modular neck materials had an impact on whole blood metal ion levels but the concentrations measured remained within the safe range at 1-year follow-up. There were no indirect signs of modular junction corrosion with either CrCo or Ti femoral necks.
Pemberian minuman formula kacang merah, kacang tanah, dan kacang kedelai terhadap status gizi ibu hamil kurang energi kronis (KEK)
Authors: Utami Nendhi Wahyunia , Majid Tita Husnitawati , Herawati Dewi Marhaeni Diah 
Published: November 2017

Background: The prevalence of malnutrition in pregnant women DIY reached 22.69%, while at Sleman amounted to 11.72%. The study has been developed drinks formula derived from beans that contain the nutrient according to the needs of pregnant women.Objective: This study aimed to analyze the influence of red beans, peanuts and soybeans drink formula against the nutritional status of pregnant women.Method: The study design is randomized controlled trial (RCT). The inclusion criteria are pregnant women with chronic energy deficiency and gestational age between 14-28 weeks. The research locations in 8 health centers in Sleman with total sample of 84 people. Data obtained by measuring LILA and pregnant women weight before and after being given a drink formula. Data analysis using Chi-Square, paired t-test and Wilcoxon.Results: There was differences between the increase of weight and LILA pregnant women in the treatment group was higher than controls (p<0.005). Increase of BB and LILA on the three groups there is a difference (p<0.001). Based on measurements of nutritional status of pregnant women showed that significant effect giving peanuts formula compared to red beans and soy bean formula that does not significantly (p<0.001). Groups of pregnant women who get peanuts formula showed increased protein intake above the standard of 67 g and a total energy of 2260 calorie.Conclusion: Nutritional status of pregnant women who get the peanut formula higher than by red beans, soy beans, and formula for pregnant women. Pregnant women who received peanuts formula have higher energy and protein intake than the other formulas.
Keseimbangan asam-basa tubuh dan kejadian sindrom metabolik pada remaja obesitas
Authors: Jauharany Firdananda Fikri , Widyastuti Nurmasari 
Published: November 2017

Background: The prevalence of metabolic syndrome increased in young population, indicated by the rise in obesity among children and adolescent. The Western dietary pattern was one of the causes. A Western diet rich in animal protein can produce acid during the metabolic process and may cause an acid-excess in the body (dietary acid load). This process was contributed to acid-base balance through the metabolism of sulfur-containing amino acids (cysteine and methionine) which produce H+ ions as well as lowering the pH.Objective: To examine the association between acid-base balance and components of metabolic syndrome among obese adolescent.Method: A cross-sectional study was conducted on 40 obese adolescents in Semarang high school. We measured Potential Renal Acid Load (PRAL) score and pH urine as an acid-base indicator. MetS are defined ≥ 3 following risk factors: waist circumference ≥90th percentile, blood pressure ≥90th percentile, triglycerides ≥110 mg/dl, HDL levels ≤40 mg/dl, and fasting blood glucose levels ≥110 mg/dl. Normality test used the Shapiro-Wilk test (n <50). The bivariate analysis used Pearson test, Rank-Spearman test, and Chi-Square test. The multivariate analysis used Multivariate Linear Regression analysis of Backward.Results: PRAL score was associated with waist circumference (r=0,347; p=0,028), sistolic blood pressure (r=0,590; p=<0,001), diastolic blood pressure (r=0,668; p=<0,001), and triglyceride levels (r=0,362; p=0,022). pH urin was not associated with any risk factors of MetS.Conclusion: High dietary acid load may be a risk factor for the development of MetS.
Análisis e implementación de diferencial GPS en configuración simple y doble
Authors: Zabala Monica , López Franklin , Ortega Angel 
Published: November 2017

El servicio de posicionamiento estándar (SPS), o medidas sin diferenciar, contiene errores en sus medidas que perturban en la solución final de posicionamiento del receptor. A través de la aplicación de técnicas de diferenciación de GPS en configuración simple (SD) y doble (DD) es posible la eliminación de errores que afectan a la señal de GPS en el trayecto de propagación, se considera los más críticos a los errores producidos por la atmósfera y tropósfera. Entre el receptor de referencia y el dispositivo receptor GPS móvil (rover) existen errores correlacionados en tiempo y espacio de acuerdo a la distancia que exista entre éstos. La eliminación de errores correlacionados incrementa la precisión en la solución final. Se diseña la aplicación denominada DGPS-Lab creada para ejecutar el proceso de diferenciación en ambas configuraciones y verificar los resultados al minimizar los errores en el posicionamiento a través del algoritmo de DGPS implementado.
PENGEMBANGAN GAME UNTUK TERAPI MEMBACA BAGI ANAK DISLEKSIA DAN DISKALKULIA
Authors: Purnomo Agus , Azizah Intan Nur , Hartono Rudi , Hartatik Hartatik , Tri Bawono Sahirul Alim 
Published: November 2017

Kesulitan belajar spesifik adalah suatu keadaan pada seorang anak yang mengalami ketidakmampuan dalam belajar, keadaan ini disebabkan gangguan proses belajar di dalam otak, yang dapat berupa gangguan persepsi (visual atau auditoris), gangguan dalam proses integratif atau gangguan ekspresif. Disleksia merupakan gangguan dalam kesulitan membaca dan Diskalkulia merupakan gangguan dalam kesulitan berhitung. Masalah muncul karena kurangnya perhatian terhadap anak yang mengalami kedua gangguan tersebut. Serta kurangnya pemahaman akan kedua gangguan tersebut terhadap anak dan belum adanya media khusus yang dapat dijadikan sebagai metode terapi terhadap kedua gangguan tersebut.Dari permasalahan diatas dibuat media pembelajaran sebagai terapi untuk anak yang mengalami gangguan dalam kesulitan membaca dan kesulitan berhitung. Media belajar yang cocok untuk menarik anak disleksia dan diskalkulia adalah dalam bentuk aplikasi game. Konsep permainan yang dikembangkan adalah anak diajak untuk mengenal huruf dan angka serta dapat membedakannya juga. Kemudian anak diajak untuk menghafalkan huruf dan angka tertentu yang menurut mereka sulit untuk dihafalkan. Dan yang terakhir anak diajak untuk menjawab pertanyaan dan menghitung dengan soal matematika dasar.Pengembangan aplikasi game ini menggunakan metodologi analisa terhadap anak disleksia dan diskalkulia. Kemudian mencari konsep game yang cocok bagi anak disleksia dan diskalkulia. Konsep tersebut digunakan untuk menentukan dalam pembuatan desain game dan background untuk diimplementasikan di unity3d. Terakhir adalah building game pada perangkat dan testing untuk kelayakan game. Aplikasi game yang telah berhasil dibuat diberi nama game “Two Dis”. Hasil yang diharapkan dari Pembuatan game “Two Dis” berbasis android ini adalah dapat menjadi salah satu media terapi dan media pembelajaran baru bagi anak disleksia dan diskalkulia dengan metode bermain game.