bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self‐Assembly of Oxamidato‐Chelated ReI‐ and MnI‐Based Flexible Dinuclear Horse‐Stirrup‐Like Metallacycles
Authors: Ramakrishna Buthanapalli , Divya D. , Monisha P. V. , Manimaran Bala. 
Published: November 2015

AbstractThe self‐assembly of M2(CO)10 (M = Mn, Re), oxamide ligands [H2L = N,N′‐dibutyloxamide (dbo), N,N′‐dibenzyloxamide (dbno)], and flexible bidentate linkers [L′ = 1,2‐bis(4‐pyridyl)ethane (bpe), 1,3‐bis(4‐pyridyl)propane (bpp)] afforded MnI‐ and ReI‐based dinuclear metallacycles [(CO)3M(μ‐η4‐L)(μ‐L′)M(CO)3] (1–6). The oxidative addition of oxamide ligands to the equatorial sites and the addition of flexible dipyridyl ligands to the axial sites of facial metal tricarbonyl cores resulted in the formation of dinuclear metallacycles under one‐pot reaction conditions. Metallacycles 1–6 were characterized through elemental analysis, IR, UV/Vis, and NMR spectroscopy. The formation of dinuclear compounds 1 and 4 was ascertained by ESI mass spectrometry. The molecular structures of 2, 3, 5, and 6 were elucidated by single‐crystal X‐ray crystallographic methods, which revealed horse‐stirrup‐like dinuclear frameworks in which the metal centers are orthogonally connected by a bridging oxamide ligand through ON∩NO bis(chelation) and a flexible bidentate ligand through M–N coordination. The first one‐step syntheses of self‐assembled neutral MnI‐based dinuclear metallastirrups containing bis(chelating) oxamide ligands and their rhenium analogues are reported herein.
Direct Electrochemical Sensing of Phenazine‐1‐carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T Using Disposable Screen‐printed Carbon Electrode
Authors: Hameed Asif , Pi Hong‐Wei , Lin Shih‐Yao , Lai Wei‐An , Young Li‐Sen , Liu You‐Cheng , Shen Fo‐Ting , Young Chiu‐Chung 
Published: November 2015

AbstractA novel electrochemical approach for direct recognition of antibiotic phenazine‐1‐carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen‐printed carbon electrode (SPCE*‐PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion‐controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*‐PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T was investigated successfully using present single run approach.
Chiral Recognition of Tryptophan Enantiomers Based on β‐Cyclodextrin‐platinum Nanoparticles/Graphene Nanohybrids Modified Electrode
Authors: Xu Juanjuan , Wang Qinghong , Xuan Chunzhi , Xia Qiao , Lin Xia , Fu Yingzi 
Published: November 2015

AbstractThe nanohybrids which based on β‐cyclodextrin, platinum nanoparticles and graphene (β‐CD‐PtNPs/GNs) were successfully synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FT‐IR) and electrochemical impedance spectroscopy (EIS). Then they were used to construct a simple and reliable chiral sensing platform to interact with tryptophan (Trp) enantiomers. Differential pulse voltammetry (DPV) was used to investigate the stereo selectivity of β‐CD‐PtNPs/GNs to Trp enantiomers. After interaction, the obvious difference of peak currents of L‐Trp and D‐Trp was obtained, indicating this strategy could be employed to chiral recognition of Trp enantiomers. Under the optimum conditions, the chiral sensor exhibited a good linear response to Trp enantiomers in a linear range of 5.0×10−5 to 5.0×10−3 M with a low limit of detection of 1.7×10−5 M (S/N=3). This approach provided a new available platform to recognize and determine Trp enantiomers.
Facile Fabrication of Solid‐state Electrochemiluminescence Sensor via Non‐covalent π‐π Stacking and Covalent Bonding on Graphite Electrode
Authors: Wang Jianmei , Xu Yuanhong , Liu Mengli , Niu Fushuang , Liu Jingquan 
Published: November 2015

AbstractHerein, a facile and efficient method was developed for fabrication of solid‐state electrochemiluminescence (ECL) sensor via non‐covalent π‐π stacking and covalent bonding on the graphite electrode (GE) surface. The electrode was firstly modified with 1‐aminopyrene via π‐π stacking between GE surface and the pyrene moiety. Thereafter a stable and efficient solid‐state ECL sensor was fabricated by covalent immobilization of ruthenium(II) onto the GE surface via amidation reaction between the 1‐aminopyrene and bis(2,2′‐bipyridyl)(4‐methyl‐4′‐carboxypropyl‐2,2′‐bipyridyl) ruthenium(II) bishexafluorophosphate. The sensor has been investigated using tripropylamine and tetracycline as representative analytes, and low detection limits of 0.7 nM and 3.5 nM (S/N=3) were reached, respectively.
Design and characterization of spatial units for monitoring global impacts of environmental factors on major crops and food security
Authors: Gommes René , Wu Bingfang , Li Zhongyuan , Zeng Hongwei 
Published: November 2015

AbstractThis paper describes the design and characterization of a limited number of areas to be used as spatial monitoring and reporting units (MRU) by CropWatch, the global crop monitoring system ( http://www.cropwatch.com.cn/) hosted by the Chinese Academy of Sciences. The MRUs are modified and adapted from an existing standard vegetation zoning map. They are designed to be areas of uniform vulnerability as assessed by three sets of variables: environmental (such as climate and NDVI), seasonality (such as intra‐annual climate variations and interannual NDVI variability) and agronomy (such as presence/absence of major crops and their yield). The paper pays due attention to within‐zone spatial variability for each of the three groups of variables: in general, the variability measured as the average of the spatial coefficient of variation inside MRUs, is much larger for agronomic variables (330%) than for environmental variables (38%) and seasonality (55%). The MRUs provide a rather coherent picture of the variations of global agriculture, for instance, the links between the distribution of crops, agricultural production potential, and environmental variability (over space and time). They closely delineate the distribution of the major nonsugar food crops (barley, cassava, maize, potatoes, rice, soybean, and wheat). The discussion focuses on the relations between the size of the MRUs and the within‐MRU variability (spatial heterogeneity) of cropping, and environmental conditions including seasonality. The conclusion stresses that spatial variability in agriculture is bound to be larger than the variability in the environmental variables used to define the units, regardless of the units’ size. The size of the spatial units (and therefore their number) is not a very critical constraint for operational impact reporting, especially if impact indicators focus on agricultural areas inside MRUs.
Dietary Saturated Fat Promotes Development of Hepatic Inflammation Through Toll‐Like Receptor 4 in Mice
Authors: Sutter Alton G. , Palanisamy Arun P. , Lench Julie H. , Esckilsen Scott , Geng Tuoyu , Lewin David N.B. , Cowart Lauren A. , Chavin Kenneth D. 
Published: November 2015

ABSTRACTNonalcoholic steatohepatitis (NASH) is currently the third most common cause of end stage liver disease necessitating transplantation. The question remains how inflammation and NASH develop in the setting of nonalcoholic fatty liver disease (NAFLD) and steatosis. Understand the roles of toll‐like receptor 4 (TLR4) and dietary fats in the development of hepatic inflammation. Wild‐type and TLR4 KO mice were fed a standard high fat diet (LD), a high saturated fat diet (MD), or an isocaloric control diet (CD). Sera and tissue were analyzed for development of hepatic steatosis, inflammation, and injury. MD induced features of hepatic steatosis and inflammation in wild‐type, but not in TLR4 KO, mice. TLR4 KO prevented MD induced increases in NAFLD activity scores, serum alanine aminotransferase levels, and inflammatory cytokine expression. Inflammatory cell infiltration and cytokine expression were also lower in the TLR4 KO mice livers than wild‐type mice fed MD. Hepatic expression of Collagen I transcripts and collagen deposition were also decreased in the TLR4 KO MD animals. Results show that TLR4 plays a critical role in the effects of dietary fat composition on the development of hepatic steatosis, inflammation, and injury consistent with nonalcoholic steatohepatitis. J. Cell. Biochem. 117: 1613–1621, 2016. © 2015 Wiley Periodicals, Inc.
Removal of heavy metals and cyanide from gold mine waste‐water by adsorption and electric adsorption
Authors: Song Yonghui , Lei Siming , Zhou Jun , Tian Yuhong 
Published: November 2015

AbstractBACKGROUNDCyanide leaching is the most widely used technology in the gold industry and this process produces large amounts of waste‐water requiring treatment before returning to the environment. There are several established techniques available to treat such toxic waste but all have some disadvantages. This study considers the use of electrical adsorption treatment of a gold mine waste‐water containing cyanide, high copper, iron, and thiocyanate content, as well as the precipitating liquid without iron.RESULTSA cell fitted with carbon electrodes made from low grade coal was used in this study and using an applied voltage of 2.0 V, plate spacing of 1 cm, and adsorption time of 24 h, the electric adsorption process provided good results on the raw cyanide waste‐water, with observed percentage removal of total cyanide (71.14), zinc (99.52) and iron (83.28). The liquid waste, following precipitation of the raw solution with zinc sulfate, was also studied and after 5 h the percentage removals of cupric ion were 90.63, 71.49 and 90.63, respectively. Analysis showed that in the process of electric adsorption, the ions in solution interacted by directional migration, enrichment precipitation and adsorption processes.CONCLUSIONSElectrical adsorption provides a suitable process for the treatment of waste‐waters from the cyanide leaching of gold. © 2015 Society of Chemical Industry
Crosslinking of peanut allergen Ara h 2 by polyphenol oxidase: digestibility and potential allergenicity assessment
Authors: Wu Zhihua , Lian Jun , Han Yuanlong , Zhou Ningling , Li Xin , Yang Anshu , Tong Ping , Chen Hongbing 
Published: November 2015

AbstractBACKGROUNDPeanut is one of the eight major food allergens. Its allergen, Ara h 2, can be recognized by over 90% of serum IgE samples from peanut‐allergic patients. Therefore, reducing the allergenicity of Ara h 2 is especially important.RESULTSIn the present study, polyphenol oxidase (PPO), a protein cross‐linking reaction catalyst that acts on tyrosine residue, was used to modify Ara h 2. After crosslinking, the microstructure, digestibility, IgG binding capability and IgE binding capability of Ara h 2 were analyzed. Cross‐linking decreased the potential allergenicity of Ara h 2 by masking the allergen epitope, while the antigenicity of Ara h 2 changed slightly. After crosslinking, the apparent diameter of Ara h 2 was altered from 300 to 1700 nm or 220 nm, indicating that polymerization could either be inter‐ or intramolecular. Regarding digestibility, crosslinked Ara h 2 was relatively more easily digested by gastric fluid compared with the untreated Ara h 2, but much more difficult in the intestinal fluid.CONCLUSIONThe crosslinking reaction catalyzed by PPO, as a non‐thermal process, may be beneficial for avoiding food allergy. The reaction could mask allergen epitopes, decreasing the allergenicity of Ara h 2. © 2015 Society of Chemical Industry
Recurrent laryngeal nerve recovery patterns assessed by serial electromyography
Authors: Paniello Randal C. , Park Andrea M. , Bhatt Neel K. , Al‐Lozi Muhammad 
Published: November 2015

Objectives/HypothesisFollowing acute injury to the recurrent laryngeal nerve (RLN), laryngeal electromyography (LEMG) is increasingly being used to determine prognosis for recovery. The LEMG findings change during the recovery process, but the timing of these changes is not well described. In this canine study, LEMGs were obtained serially following model RLN injuries.Study DesignAnimal Study.MethodsThirty‐six canine RLNs underwent crush (n = 6), complete transection with reanastomosis (n = 6), half‐transection half‐crush (n = 5), cautery (n = 5), stretch (n = 5), inferior crush (n = 4), or inferior transection with reanastomosis (n = 5) injuries. Injuries were performed 5 cm from cricoid or were 5 cm further inferior. Under light sedation, LEMG of thyroarytenoid muscles was performed monthly for 6 months following injury. At 6 months, spontaneous and induced vocal fold motion was assessed.ResultsExcept for the stretch injury, the remaining groups showed very similar recovery patterns. Fibrillation potentials (FPs) and/or positive sharp waves (PSWs; signs of bad prognosis) were seen in all cases at 1 month and lasted on average for 2.26 months (range = 1–4 months). Motor unit potentials of at least 2+ (scale = 0–4+; signs of good prognosis) were seen beginning at 3.61 months (range = 2–6 months). The stretch injury was less severe, with 3 of 5 showing no FPs/PSWs at 1 month; all recovered full mobility. Ten of the 36 thyroarytenoid muscles (27.8%) had 1 electromyograph showing both bad prognosis and good prognosis signs simultaneously at 2 to 4 months postinjury.ConclusionsLEMG can be used to predict RNL recovery, but timing is important and LEMG results earlier than 3 months may overestimate a negative prognosis.Level of EvidenceNA Laryngoscope, 126:651–656, 2016
Clinical observations and molecular variables of patients with hearing loss and incomplete partition type III
Authors: Choi Byung Yoon , An Yong‐Hwi , Song Jae‐Jin , Koo Ja‐Won , Lee Jun Ho , Oh Seung Ha , Chang Sun O. , Kim Chong Sun , Park Joo Hyun 
Published: November 2015

Objectives/HypothesisTo analyze the clinical manifestations and genetic features of patients with hearing loss (HL) and incomplete partition (IP) type III malformation, and to evaluate speech performance after cochlear implantation (CI) in these patients.Study DesignIndividual retrospective cohort study.MethodsOf 206 probands with inner ear malformations (IEMs), we constructed a homogeneous cohort of 11 genetically documented IP type III (DFNX2). Mutations affecting POU3F4 were classified as extension (n = 2), truncation (n = 3), large genomic deletion (n = 2), or missense substitution (n = 4). Postoperative outcomes were rigorously assessed with focus on POU3F4 genotypes and compared with 80 age‐matched implantees without IEMs.ResultsHL in our cohort was prelingual in onset irrespective of degree. Serviceable hearing was obtained by wearing conventional hearing aids in three, and eight subjects required CI. No correlation was found between mutation types and initial auditory phenotype. As for the eight cochlear implantees, average Categories of Auditory Perception score was <1 preoperatively and 3.9 at 2 years post‐CI. Speech performances improved over the first 3 months postoperatively in a manner comparable to implantees without IEMs. However, it then tended to slow down until 1 year postoperatively, leading to worse scores at 2 years than implantees without IEMs. Furthermore, this was more evident in those with a truncation or deletion mutation.ConclusionsCI surgeons should be aware that postoperative auditory performance may be not as good in IP type III patients as in patients without IEMs. In particular, our study implies that certain types of POU3F4 mutations may have poorer prognoses than other types after CI.Level of Evidence4. Laryngoscope, 126:E123–E128, 2016
Impact of patient‐related factors on the outcomes of office‐based injection laryngoplasty
Authors: Sardesai Maya G. , Merati Albert L. , Hu Amanda , Birkent Hakan 
Published: November 2015

Objectives/HypothesisIn‐office percutaneous injection laryngoplasty (IL) is a common treatment for glottal insufficiency. The objective of this prospective study was to determine if voice outcomes from IL are affected by age, gender, or initial disease severity.Study DesignProspective case series.MethodsConsecutive adult patients undergoing awake injection laryngoplasty were recruited from a subspecialty laryngology clinic. Voice Handicap Index (VHI‐30); Consensus Auditory‐Perceptual Evaluation of Voice (CAPE‐V); and Grade, Roughness, Breathiness, Asthenia and Strain (GRBAS) Dysphonia Scale were evaluated prior to and 2 months after injection. Patients who had more severe disease were defined as those patients with greater initial perception of handicap, with a VHI score greater than 60.ResultsThirty‐five subjects were enrolled, and 27 (16 male; mean age 61.6 ± 13.2 years) had complete data. No impact from age or gender was seen on outcomes from IL as measured by VHI‐30 (P = 0.397 for age; P = 0.764 for gender), CAPE‐V (P = 0.675 for age, P = 0.975 for gender), or GRBAS (P = 0.213 for age, P = 0.983 for gender). Patients with poorer initial VHI tended to have more significant improvement (P = 0.002), which may represent a ceiling effect.ConclusionsIn this prospective clinical study, age and gender did not affect outcome in IL. Patients with more severe disease appeared to have greater improvement.Level of Evidence4. Laryngoscope, 126:1806–1809, 2016
Altered incubation temperatures between embryonic Days 7 and 13 influence the weights and the mitochondrial respiratory and enzyme activities in breast and leg muscles of broiler embryos
Authors: Krischek Carsten , Janisch Sabine , Naraballobh Watcharapong , Brunner Ronald , Wimmers Klaus , Wicke Michael 
Published: November 2015

SUMMARYAltering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryonic Day (ED) 7 and 10 or ED 10 and 13, which cover critical periods of embryonic myogenesis. The embryos were then dissected immediately after treatment on ED 10 or 13 to assess body, liver, and heart weights as well as to analyze breast and leg muscle fibers for their mitochondrial respiratory activity (MRA). Breast muscle samples were additionally used to evaluate the activity of enzymes involved in energy metabolism and cell‐cycle progression. ED‐10 embryos incubated at 38.8°C showed elevated weights (body, liver, and heart), MRA, and activities of lactate dehydrogenase and cytochrome oxidase compared to the ED‐10 embryos incubated at 36.8°C. Similarly, the ED‐13 embryos incubated at 38.8°C showed elevated body weight, MRA, and activities of glycogen phosphorylase, phosphofructokinase, and cytochrome oxidase compared to their 36.8°C counterparts. Embryos incubated at the normal temperature (37.8°C), however, showed variable differences from those incubated at 38.8°C versus 36.8°C. Cell‐cycle enzyme activities were not impacted by the different temperature treatments. Thus, an increase or decrease in the incubation temperature during embryonic broiler myogenesis results in altered embryo activity, muscle energy metabolism, and activity‐dependent muscle growth. Mol. Reprod. Dev. 83: 71–78, 2016. © 2015 Wiley Periodicals, Inc.
Position and orientation measurement of susceptibility markers using spectrally selective spin‐echo projections
Authors: Karimi Hirad , Dominguez‐Viqueira William , Cunningham Charles H. 
Published: November 2015

PurposeTo develop an accurate technique for simultaneously measuring the position and orientation of interventional devices using a projection‐based spectrally selective refocusing pulse sequence.MethodsProjections along physical axes using spectrally selective excitation were acquired to track a catheter. A 9F passive tracking device capable of generating controllable susceptibility artifacts using susceptibility materials (titanium and graphite) was attached to the catheter. A library of projections for different orientations of the device with respect to the main magnetic field were simulated offline. Cross‐correlations with these templates were computed to determine the orientation and position of the device. A phantom study was performed to evaluate the accuracy of the tracking technique. The tracking technique was also evaluated in vivo in the carotid artery of a pig.ResultsSimultaneous and accurate measurement of position and orientation of the tracking device was obtained in the phantom and in vivo studies with reasonable temporal resolution. For the phantom study, the average of absolute errors in the Z‐, Y‐, and X‐axes are 0.37, 0.76, and 0.85 mm, respectively. The mean absolute error and standard deviation of orientation measurement are 1.5 and 1.1 degrees, respectively.ConclusionThis positioning technique, in conjunction with a controllable tracking device, can provide accurate tracking of interventional devices in MR‐guided interventions. Magn Reson Med 76:1563–1573, 2016. © 2015 International Society for Magnetic Resonance in Medicine
Coronary endothelial function assessment using self‐gated cardiac cine MRI and k‐t sparse SENSE
Authors: Yerly Jérôme , Ginami Giulia , Nordio Giovanna , Coristine Andrew J. , Coppo Simone , Monney Pierre , Stuber Matthias 
Published: November 2015

PurposeElectrocardiogram (ECG)‐gated cine MRI, paired with isometric handgrip exercise, can be used to accurately, reproducibly, and noninvasively measure coronary endothelial function (CEF). Obtaining a reliable ECG signal at higher field strengths, however, can be challenging due to rapid gradient switching and an increased heart rate under stress. To address these limitations, we present a self‐gated cardiac cine MRI framework for CEF measurements that operates without ECG signal.MethodsCross‐sectional slices of the right coronary artery (RCA) were acquired using a two‐dimensional golden angle radial trajectory. This sampling approach, combined with the k‐t sparse SENSE algorithm, allows for the reconstruction of both real‐time images for self‐gating signal calculations and retrospectively reordered self‐gated cine images. CEF measurements were quantitatively compared using both the self‐gated and the standard ECG‐gated approach.ResultsSelf‐gated cine images with high‐quality, temporal, and spatial resolution were reconstructed for 18 healthy volunteers. CEF as measured in self‐gated images was in good agreement (R2 = 0.60) with that measured by its standard ECG‐gated counterpart.ConclusionHigh spatial and temporal resolution cross‐sectional cine images of the RCA can be obtained without ECG signal. The coronary vasomotor response to handgrip exercise compares favorably with that obtained with the standard ECG‐gated method. Magn Reson Med 76:1443–1454, 2015. © 2015 International Society for Magnetic Resonance in Medicine