bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights from the draft genome of the subsection V (Stigonematales) cyanobacterium Hapalosiphon sp. Strain MRB220 associated with 2-MIB production
Authors: Tan Boon Fei , Te Shu Harn , Boo Chek Yin , Gin Karina Yew-Hoong , Thompson Janelle Renee 
Published: September 2016

AbstractA non-axenic unialgal culture containing a Subsection V (Stigonematales) cyanobacterium, Hapalosiphon strain MRB 220, was obtained from a benthic freshwater algal mat through multiple transfers following growth in sterile media. Physiological characterization demonstrated the culture was capable of nitrogen-fixation and production of the off flavor compound 2-methylisoborneol (2-MIB). Total DNA isolated from this culture was sequenced using Illumina HiSeq and de novo assembled into contigs. The genome of MRB 220 was separated from co-occurring heterotrophic bacteria using sequence homology and compositional approaches, and its purity was confirmed based on best BLAST hit classification and principle component analysis of the tetranucleotide frequencies of fragmented contigs. The genome of ~7.4 Mbp contains 6,345 protein coding genes with 4,320 of these having functional prediction including predicted pathways for biosynthesis of the secondary metabolite welwitindolinone. Analyses of 16S rRNA gene and whole genome sequence average nucleotide identity indicated close relatedness of MRB 220 to the genera Hapalosiphon and Fischerella within the order Stigonematales. Microscopic examination showed that MRB 220 formed heterocystous branched filaments, thereby supporting identification of strain MRB 220 as a morphospecies of Hapalosiphon. Availability of the draft genome of Hapalosiphon strain MRB 220 enables future work to elucidate the pathway and dynamics for biosynthesis of 2-MIB and other secondary metabolites and understand the ecology and physiology of Stigonematales cyanobacteria in tropical freshwaters.
High quality draft genome sequences of Pseudomonas fulva DSM 17717T, Pseudomonas parafulva DSM 17004T and Pseudomonas cremoricolorata DSM 17059T type strains
Authors: Peña Arantxa , Busquets Antonio , Gomila Margarita , Mulet Magdalena , Gomila Rosa M. , Reddy T. B. K. , Huntemann Marcel , Pati Amrita , Ivanova Natalia , Markowitz Victor , García-Valdés Elena , Göker Markus , Woyke Tanja , Klenk Hans-Peter , Kyrpides Nikos , Lalucat Jorge 
Published: September 2016

AbstractPseudomonas has the highest number of species out of any genus of Gram-negative bacteria and is phylogenetically divided into several groups. The Pseudomonas putida phylogenetic branch includes at least 13 species of environmental and industrial interest, plant-associated bacteria, insect pathogens, and even some members that have been found in clinical specimens. In the context of the Genomic Encyclopedia of Bacteria and Archaea project, we present the permanent, high-quality draft genomes of the type strains of 3 taxonomically and ecologically closely related species in the Pseudomonas putida phylogenetic branch: Pseudomonas fulva DSM 17717T, Pseudomonas parafulva DSM 17004T and Pseudomonas cremoricolorata DSM 17059T. All three genomes are comparable in size (4.6–4.9 Mb), with 4,119–4,459 protein-coding genes. Average nucleotide identity based on BLAST comparisons and digital genome-to-genome distance calculations are in good agreement with experimental DNA-DNA hybridization results. The genome sequences presented here will be very helpful in elucidating the taxonomy, phylogeny and evolution of the Pseudomonas putida species complex.
The physical and physiological effects of vacuum massage on the different skin layers: a current status of the literature
Authors: Moortgat Peter , Anthonissen Mieke , Meirte Jill , Van Daele Ulrike , Maertens Koen 
Published: September 2016

AbstractVacuum massage is a non-invasive mechanical massage technique performed with a mechanical device that lifts the skin by means of suction, creates a skin fold and mobilises that skin fold. In the late 1970s, this therapy was introduced to treat traumatic or burn scars.Although vacuum massage was invented to treat burns and scars, one can find very little literature on the effects of this intervention. Therefore, the aim of this review is to present an overview of the available literature on the physical and physiological effects of vacuum massage on epidermal and dermal skin structures in order to find the underlying working mechanisms that could benefit the healing of burns and scars. The discussion contains translational analysis of the results and provides recommendations for future research on the topic.An extended search for publications was performed using PubMed, Web of Science and Google Scholar. Two authors independently identified and checked each study against the inclusion criteria.Nineteen articles were included in the qualitative synthesis. The two most reported physical effects of vacuum massage were improvement of the tissue hardness and the elasticity of the skin. Besides physical effects, a variety of physiological effects are reported in literature, for example, an increased number of fibroblasts and collagen fibres accompanied by an alteration of fibroblast phenotype and collagen orientation. Little information was found on the decrease of pain and itch due to vacuum massage.Although vacuum massage initially had been developed for the treatment of burn scars, this literature review found little evidence for the efficacy of this treatment. Variations in duration, amplitude or frequency of the treatment have a substantial influence on collagen restructuring and reorientation, thus implying possible beneficial influences on the healing potential by mechanotransduction pathways. Vacuum massage may release the mechanical tension associated with scar retraction and thus induce apoptosis of myofibroblasts. Suggestions for future research include upscaling the study design, investigating the molecular pathways and dose dependency, comparing effects in different stages of repair, including evolutive parameters and the use of more objective assessment tools.
Neutrophils can disarm NK cell response through cleavage of NKp46
Authors: Valayer Alexandre , Brea Deborah , Lajoie Laurie , Avezard Leslie , Combes-Soia Lucie , Labas Valerie , Korkmaz Brice , Thibault Gilles , Baranek Thomas , Si-Tahar Mustapha 
Published: September 2016

Abstract
Polymorphonuclear neutrophils (PMNs) can contribute to the regulation of the host immune response by crosstalk with innate and adaptive leukocytes, including NK cells. Mechanisms by which this immunoregulation process occurs remain incompletely understood. Here, we focused on the effect of human neutrophil-derived serine proteases on NKp46, a crucial activating receptor expressed on NK cells. We used flow cytometry, Western blotting, and mass spectrometry (MS) analysis to reveal that cathepsin G [CG; and not elastase or proteinase 3 (PR3)] induces a time- and concentration-dependent, down-regulatory effect on NKp46 expression through a restricted proteolytic mechanism. We also used a functional assay to demonstrate that NKp46 cleavage by CG severely impairs NKp46-mediated responses of NK cells, including IFN-γ production and cell degranulation. Importantly, sputa of cystic fibrosis (CF) patients, which have high concentrations of CG, also alter NKp46 on NK cells. Hence, we have identified a new immunoregulatory mechanism of neutrophils that proteolytically disarms NK cell responses.
Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06
Authors: Yonemura Yoji , Li Xiaolin , Müller Katja , Krämer Andreas , Atigbire Paul , Mentrup Torben , Feuerhake Talitha , Kroll Torsten , Shomron Olga , Nohl Richard , Arndt Hans-Dieter , Hoischen Christian , Hemmerich Peter , Hirschberg Koret , Kaether Christoph 
Published: September 2016

ABSTRACT
Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and handed over to the Sar1 and COPII machinery in ERES, and a capture model, where cargo freely diffuses into ERES where it is captured by the Sar1 and COPII machinery. Using the novel secretion inhibitor FLI-06, we show that recruitment of the cargo VSVG to ERES is an active process upstream of Sar1 and COPII. Applying FLI-06 before concentration of VSVG in ERES completely abolishes its recruitment. In contrast, applying FLI-06 after VSVG concentration in ERES does not lead to dispersal of the concentrated VSVG, arguing that it inhibits recruitment to ERES as opposed to capture in ERES. FLI-06 also inhibits export out of the trans-Golgi network (TGN), suggesting that similar mechanisms might orchestrate cargo selection and concentration at the ER and TGN. FLI-06 does not inhibit autophagosome biogenesis and the ER-peroxisomal transport route, suggesting that these rely on different mechanisms.
Venous thromboembolism in the elderly
Authors: Ploenes C. 
Published: September 2016

SummaryOld age is an independent risk factor of venous thromboembolism. Nevertheless initial symptoms are often attributed to existing cardiac or pulmonary comorbidity. Once deep thromboembolism (DTE) is in focus, the synopsis of clinical findings and anamnestic clues help to take further steps to establish or rule out the diagnosis (e.g. Wells score). Treatment consists in oral anticoagulation, either by vitamin-k-antagonists or by direct oral anticoagulants (“DOACs”). Strict compliance of patients or main caregivers is essential in both cases. Simultaneous medication of platelet-inhibitingor nonsteroidal anti-inflammatory drugs – often unknown self medication – results in a raised bleeding risk and should be avoided. If longterm anticoagulation is mandatory, a strategy of sequential dose-reduced anticoagulation can be considered, especially in the case of increased bleeding-risk. Systemic fibrinolysis of pulmonary embolism goes along with a very high bleeding risk in old age and should be performed only in case of vital circulatory depression or failure.
Surgical repair of a popliteal vein aneurysm to prevent thromboembolic complications
Authors: Mühlberger D. , Regeniter P. , Mumme A. , Hummel T. , Burkert B. 
Published: September 2016

SummaryThe case reported here involves a 72-year-old female patient with right-sided popliteal vein aneurysm, which became apparent during follow-up ultrasound scanning after deep venous thrombosis of the left leg with pulmonary embolism. Due to the size of the aneurysm, the decision was made to perform surgery. The aneurysm was tangentially resected by means of plication. Aneurysms of the deep venous system carry a considerable risk of a thromboembolic event and therefore require treatment, even when they are asymptomatic. Surgical treatment of an aneurysm of the deep leg veins is a safe and low-risk procedure for the prevention of pulmonary embolism.
The post-thrombotic syndrome
Authors: Pannier F. , Rabe E. 
Published: September 2016

SummaryPost-thrombotic syndrome (PTS) is a common complication of deep vein thrombosis (DVT) in the legs. Risk factors include recurrent ipsilateral DVT, proximal vein involvement, persistent occlusion of the deep venous system, obesity, and pre-existing chronic venous disease. Compression therapy, exercise, and weight loss, as well as effective anticoagulation of the initial DVT and secondary thromboprophylaxis, are all important aspects in the prevention and treatment of post-thrombotic syndrome.
Bayesian Estimation of a Dynamic Model of Two-Sided Markets: Application to the U.S. Video Game Industry
Authors: Zhou Yiyi 
Published: September 2016

This paper develops and estimates a structural model of two-sided markets with durable platform intermediaries and affiliated products. It models buyers’ purchase decisions of platforms and affiliated products and sellers’ decisions of price setting and entry, accounting for the dynamic interaction between the two distinct groups of platform participants. To estimate the proposed model, this paper develops a Bayesian Markov chain Monte Carlo estimation approach that incorporates nonparametric approximation and interpolation methods. The proposed model and estimation method are applied to the 32/64-bit generation of the U.S. video game industry. The results of counterfactual experiments show that the dynamic behavior of platform participants has significant impacts on platform adoption and the affiliated product market, and that a failed platform could have survived if it had priced the two sides properly in a dynamic two-sided market environment. This paper was accepted by Matthew Shum, marketing.
The combined effect of administration of intravenous and topical tranexamic acid on blood loss and transfusion rate in total knee arthroplasty
Authors: Yuan Z. F. , Yin H. , Ma W. P. , Xing D. L. 
Published: September 2016

Objectives Tranexamic acid (TXA) is an antifibrinolytic agent used as a blood-sparing technique in total knee arthroplasty (TKA), and is routinely administered by intravenous (IV) or intra-articular (IA) injection. Recently, a novel method of TXA administration, the combined IV and IA application of TXA, has been applied in TKA. However, the scientific evidence of combined administration of TXA in TKA is still meagre. This meta-analysis aimed to investigate the efficacy and safety of combined IV and IA TXA in patients undergoing TKA. Materials and Methods A systematic search was carried out in PubMed, the Cochrane Clinical Trial Register (Issue12 2015), Embase, Web of Science and the Chinese Biomedical Database. Only randomised controlled trials (RCT) evaluating the efficacy and safety of combined use TXA in TKA were identified. Two authors independently identified the eligible studies, extracted data and assessed the methodological quality of included studies. Meta-analysis was conducted using Review Manager 5.3 software. Results A total of ten RCTs (1143 patients) were included in this study. All the included studies were randomised and the quality of included studies still needed improvement. The results indicated that, compared with either placebo or the single-dose TXA (IV or IA) group, the combination of IV and IA TXA group had significantly less total blood loss, hidden blood loss, total drain output, a lower transfusion rate and a lower drop in haemoglobin level. There were no statistically significant differences in complications such as wound infection and deep vein thrombosis between the combination group and the placebo or single-dose TXA group. Conclusions Compared with placebo or the single-dose TXA, the combined use of IV and IA TXA provided significantly better results with respect to all outcomes related to post-operative blood loss without increasing the risk of thromboembolic complications in TKA. Cite this article: Z. F. Yuan, H. Yin, W. P. Ma, D. L. Xing. The combined effect of administration of intravenous and topical tranexamic acid on blood loss and transfusion rate in total knee arthroplasty: combined tranexamic acid for TKA. Bone Joint Res 2016;5:353–361. DOI: 10.1302/2046-3758.58.BJR-2016-0001.R2.
Dieta da Gurijuba - Sciades parkeri - Desembarcada pelas pescarias artesanais de larga escala da costa norte do Brasil
Authors: Duarte Paula Jeanne , Nogueira Luciane de Carvalho , Pimentel Zélia Maria Nunes , Bentes Bianca 
Published: September 2016

Sciades parkeri é um peixe marinho/estuarino conhecido popularmente como gurijuba. Para o estudo da dieta foram coletadas amostras bimestrais, na costa do Amapá, entre setembro de 2011 e dezembro de 2012. Em laboratório, os estômagos foram removidos, pesados e seus conteúdos gástricos foram analisados com auxílio de estereomicroscópio. A dieta foi avaliada a partir de métodos de frequência de ocorrência, índice de análise volumétrica e índice de importância alimentar. Foram analisados 189 estômagos; destes, 139 continham alimentos, 25 continham sedimento, 25 estavam vazios. Os itens alimentares principais foram fragmentos de crustáceos (n=60) peixes (n=33), Cycloplax pinnotheroides (Brachyura) (n=22), Os itens que apresentara as maiores frequências de ocorrência e índice de importância alimentar tanto no período chuvoso quanto no seco foram fragmentos de crustáceos (chuvoso Fi=38,64% e AIi= 65,35% e seco Fi = 48,15% e AIi= 67,76%) e fragmentos de peixes (chuvoso Fi=20,45% e AIi= 19,89% e seco Fi = 27,78% e AIi= 23,45%). As análises indicaram que a S. parkeri é uma espécie essencialmente carnívora.
El programa EDUCO, las evaluaciones de impacto, y la economía política de la reforma educativa global
Authors: Edwards, Jr. D. Brent , Loucel Urquilla Claudia Elizabeth 
Published: September 2016

a en El Salvador, sino además se convirtió en una política educativa de carácter global, conocida por descentralizar, hacia las familias rurales, la responsabilidad de contratar y despedir maestros. Como se presenta en este artículo, su ascenso a la fama no fue producto únicamente del contexto político-económico en el que nació; su fama también es el resultado de las evaluaciones de impacto producidas por el Banco Mundial, que sirvieron como base de evidencia a través de la cual ésta y otras instituciones internacionales podían promover, de forma legítima, el modelo neoliberal de participación de la comunidad representado por EDUCO. Sin embargo, de una manera problemática, una re-apreciación de estas evaluaciones de impacto, revela, en primer lugar, que los hallazgos y conclusiones sobre los efectos significativos de EDUCO no fueron justificados, y, en segundo lugar, que la producción de las evaluaciones de impacto en su conjunto es fundamentalmente defectuosa debido al nexo intelectual-político-financiero del cual emergieron estos estudios, y hacia el cual retornaron para promover—alrededor del mundo—soluciones de políticas orientadas al mercado. En este sentido, para explicar las dinámicas de la reforma que dieron lugar a EDUCO, este documento (a) revisa sistemáticamente los hallazgos y limitantes de cada uno de los seis estudios de impacto que constituyen la base internacional de conocimiento alrededor de esta política, (b) reconsidera lo que, de forma razonable, podemos exigir saber sobre EDUCO, (c) refleja las implicaciones nacionales e internacionales de la revisión crítica aquí presentada (d) resalta las falencias del método de investigación de las evaluaciones de impacto y (e) sugiere varios métodos alternativos que pueden ofrecer hallazgos relevantes para las políticas.
EFFICACY OF CURCUMIN AND MONOCHROMATIC LIGHT IN IMPROVING LIVER FUNCTIONS OF SEXUALLY MATURE MAGELANG DUCKS
Authors: Kasiyati K. , Manalu W. , Sumiati S. , Ekastuti D. R. 
Published: September 2016

The liver of female poultry has a vital role as an organ for biosynthesis of the yolk precursor and has a greater chance of experiencing cellular damages. This experiment was designed to study the uses of curcumin and monochromatic light in suppressing liver destruction during the period of yolk biosynthesis in Magelang ducks. One hundred and ninety two female Magelang ducks were assigned into a completely randomized design with a 4x4 factorial arrangement. The first factor was dose of curcumin supplementation consisted of 4 levels i.e., 0, 9, 18, and 36 mg. The second factor was the color of monochromatic light consisted of 4 levels i.e., white, red, green, and blue. The results showed that hydropic and fat degenerations in the livers of Magelang ducks entering laying phase were still in a normal range. There was an interaction effect between curcumin and monochromatic light on the serum SGPT/GOT and liver MDA concentrations (P<0.05). Serum lipid concentrations positively correlated with the increased serum SGPT/GOT and liver MDA concentrations as indicators of liver damages. Supplementation of curcumin and the use of monochromatic light could reduce and suppress liver destruction and damage during laying period.
Tangent Lines and Lipschitz Differentiability
Spaces
Authors: Cavalletti Fabio , Rajala Tapio 
Published: September 2016

Abstract
We study the existence of tangent lines, i.e. subsets of the tangent space isometric to the real line,
in tangent spaces of metric spaces.We first revisit the almost everywhere metric differentiability of Lipschitz
continuous curves. We then show that any blow-up done at a point of metric differentiability and of density
one for the domain of the curve gives a tangent line.
Metric differentiability enjoys a Borel measurability property and this will permit us to use it in the framework
of Lipschitz differentiability spaces.We show that any tangent space of a Lipschitz differentiability space contains
at least n distinct tangent lines, obtained as the blow-up of n Lipschitz curves, where n is the dimension
of the local measurable chart. Under additional assumptions on the space, such as curvature lower bounds,
these n distinct tangent lines span an n-dimensional part of the tangent space.
Infinitesimal Structure of Differentiability
Spaces, and Metric Differentiation
Authors: Cheeger Jeff , Kleiner Bruce , Schioppa Andrea 
Published: September 2016

Abstract
We prove metric differentiation for differentiability spaces in the sense of Cheeger [10, 14, 27]. As
corollarieswe give a new proof of one of the main results of [14], a proof that the Lip-lip constant of any Lip-lip
space in the sense of Keith [27] is equal to 1, and new nonembeddability results.
A Generalized Approach for the Modeling of Goodwin-Type Cycles
Authors: Madotto Matteo , Gaudenzi Marcellino , Zanolin Fabio 
Published: September 2016

Abstract
Goodwin’s celebrated growth cycle model has been widely studied since its introduction in 1967. In recent years several contributions have appeared with the aim of amending the original model so as to improve its economic coherence and enrich its structure. In this article we propose a new and generalized approach, within the theory of planar Hamiltonian systems, for the modeling of Goodwin-type cycles. This new approach, which includes and improves various attempts by the recent literature, is very general and fulfills the essential requirement that the orbits lie entirely in the economically feasible interval. We provide a necessary and sufficient condition for all solutions to be cycles lying entirely in the unit box. In addition, we study the period length of the cycles near the equilibrium and close to the boundary of the domain. Finally, we discuss an example of how small perturbations of the model may affect the qualitative behavior of the solutions.
MC1R gene variants involvement in human OCA phenotype
Authors: Saleha Shamim , Khan Taj Ali , Zafar Shaista 
Published: September 2016

AbstractOculocutaneous albinism (OCA) is a genetic disorder of melanin synthesis that results in hypopigmentation in hair, skin and eyes. OCA has been reported in individuals from all ethnic backgrounds but it is more common among those with Europeans ancestry. OCA is heterogeneous group of disorders and seven types of OCA are caused by mutations in TYR (OCA1), OCA2 (OCA2), TYRP1 (OCA3), SLC45A2 (OCA4), SLC24A5 (OCA6) and C10oRF11 (OCA7) genes. However, MC1R gene variants have been reported that modify OCA2 phenotype but the knowledge about the function ofMC1R gene in melanogenesis, and genotype-phenotype association, in case of OCA, is limited. In this review article we present a comprehensive description of classification of OCA, role of MSH-R in melanin synthesis, the sequence variations in MC1R and their association with OCA. This review will enhance our understanding of MC1R gene variants involved in human OCA2 phenotype.