bluuebunny/crossref_metadata_2025_split huggingface dataset:
A temporary deficiency in self‐control: Can heightened motivation overcome this effect?
Authors: Kelly Claire L. , Crawford Trevor J. , Gowen Emma , Richardson Kelly , Sünram‐Lea Sandra I. 
Published: January 2017

AbstractSelf‐control is important for everyday life and involves behavioral regulation. Self‐control requires effort, and when completing two successive self‐control tasks, there is typically a temporary drop in performance in the second task. High self‐reported motivation and being made self‐aware somewhat counteract this effect—with the result that performance in the second task is enhanced. The current study explored the relationship between self‐awareness and motivation on sequential self‐control task performance. Before employing self‐control in an antisaccade task, participants initially applied self‐control in an incongruent Stroop task or completed a control task. After the Stroop task, participants unscrambled sentences that primed self‐awareness (each started with the word “I”) or unscrambled neutral sentences. Motivation was measured after the antisaccade task. Findings revealed that, after exerting self‐control in the incongruent Stroop task, motivation predicted erroneous responses in the antisaccade task for those that unscrambled neutral sentences, and high motivation led to fewer errors. Those primed with self‐awareness were somewhat more motivated overall, but motivation did not significantly predict antisaccade performance. Supporting the resource allocation account, if one was motivated—intrinsically or via the manipulation of self‐awareness—resources were allocated to both tasks leading to the successful completion of two sequential self‐control tasks.
Endocrine and paracrine controls of canine follicular development and function
Authors: Songsasen N , Nagashima J , Thongkittidilok C 
Published: January 2017

ContentCanid reproduction is unique among other mammals in that females experience long and variable periods of ovarian inactivity. While the domestic dog exhibits a non‐seasonal, largely sporadic monoestrus occurring once or twice a year, most wild canids, such as the gray wolf (Canis lupus) and red wolf (Canis rufus), are seasonal breeders with onset apparently dependent on species, latitudinal location and/or variety of environment factors. Neuroendocrine controls of ovarian functions have been mostly studied in the dog, but less so in their wild counterparts, due to difficulties in regular blood sampling. Yet, development of non‐invasive hormone monitoring has advanced the understanding of reproductive cycle in wild canids. Recent advances in in vitro follicle culture technology also have begun to provide insights into paracrine controls of canid ovarian folliculogenesis. This review highlights current knowledge on canid reproduction with emphasis on endocrine and paracrine controls of follicular development. We also discuss future research priorities, including advancing the understanding of anoestrous termination and role of paracrine factors in canine folliculogenesis.
Explaining Caste Differences in Private School Attendance
Authors: Azam Mehtabul 
Published: January 2017

AbstractUsing a nationally representative education survey, we explore caste differences in private school attendance in India. We find lower private school attendance among the disadvantaged castes—Scheduled Castes (SCs), Scheduled Tribes (STs) and Other Backward Castes (OBCs)—when compared with the non‐disadvantaged group (non‐SC/ST/OBC). Controlling for geographical location, household and individual level factors reduces the gaps in private school attendance considerably; however, the gaps remain quite large. We find that variation in parental education and household consumption expenditure explains a significant proportion of the observed gaps. For ST students, geographical location remains important in explaining the gap in private school attendance.
Investigation of Genetic Defects in Severe Combined Immunodeficiency Patients from Turkey by Targeted Sequencing
Authors: Erman B. , Bilic I. , Hirschmugl T. , Salzer E. , Boztug H. , Sanal Ö. , Çağdaş Ayvaz D. , Tezcan I. , Boztug K. 
Published: January 2017

AbstractPrimary immunodeficiencies (PIDs) represent a large group of disorders with an increased susceptibility to infections. Severe combined immunodeficiency (SCID) is the most severe form of primary immunodeficiencies (PIDs) with marked T‐cell lymphopenia. Investigation of the genetic aetiology using classical Sanger sequencing is associated with considerable diagnostic delay. We here established a custom‐designed, next‐generation sequencing (NGS)‐based panel to efficiently identify disease‐causing genetic defects in PID patients and applied this method in SCID patients of Turkish origin with previously undefined genetic aetiology. We used HaloPlex enrichment technology, a targeted, NGS‐based method which was designed to diagnose patients with SCID and other PIDs. Our HaloPlex panel included a total of 356 PID‐related genes, and we searched disease‐causing mutations in 19 Turkish SCID patients without a genetic diagnosis. The coverage of targeted regions ranged from 97.47% to 99.62% with an average of 98.31% for all patients. All known SCID genes were covered with a percentage of at least 97.3%. We made a genetic diagnosis in six of 19 (33%) patients, including four novel disease‐causing mutations identified in RAG1, JAK3 and IL2RG, respectively. We showed that this NGS‐based method can provide rapid genetic diagnosis for patients suffering from SCID, potentially facilitating clinical treatment decisions.
How (Not) to Elide Negation
Authors: van Craenenbroeck Jeroen , Temmerman Tanja 
Published: January 2017

AbstractThis paper examines the behavior of English negative indefinites under VP ellipsis. The main empirical observation is that negative indefinites cannot take scope out of a verbal ellipsis site. We propose that negative indefinites involve fusion under adjacency between the clausal polarity head and an indefinite determiner and that this adjacency comes about under multidominance. Multidominance can feed the morphological coalescence of two syntactic terminals that on the surface do not appear to be linearly adjacent. The claim that there is a morphological relation between these two heads—rather than a syntactic one—is supported by the fact that it can be bled by ellipsis. Given that ellipsis is a process at Phonological Form (PF), it can block fusion, thus preventing high scope of negative indefinites out of an ellipsis site.
Analysis of synovial fluid of the Capybara's stifle joints
Authors: Brombini Giovanna C. , Rahal Sheila C. , Bergamini Bruno C. S. , Lopes Raimundo S. , Santos Ivan F. C. , Schimming Bruno C. 
Published: January 2017

BackgroundAlthough normal synovial fluid has been well characterized in domestic animals such as dogs, cats, horses, and cows, the available information on larger rodents is scarce.ObjectivesThe purpose of the study was to analyze the physical, chemical, and cytologic characteristics of the synovial fluid in stifle joints of Capybaras.MethodsFive free‐ranging adult female Capybaras (Hydrochoerus hydrochaeris), weighing from 37 to 56 kg were used. Synovial fluid was obtained by aspiration of 10 stifle joints. Samples were analyzed for physical, chemical, and cytologic properties.ResultsSpontaneous clotting was negative in 9 samples. Most synovial fluids had pH 8, and protein concentrations ranged from 1.6 to 3.6 g/dL. The mucin clot test was good in all 6 samples that were tested. Nucleated cell counts ranged from 140 to 508 cells/μL. Relative differential leukocyte counts demonstrated a predominance of mononuclear cells (97.6%), including 76.2% undifferentiated mononuclear cells, 18.1% macrophages, and 3.66% lymphocytes. Polymorphonuclear cells included 1.83% neutrophils and 0.2% eosinophils.ConclusionThe synovial stifle joint fluid of healthy free‐ranging adult Capybaras is clear, colorless, viscous, and with chemical features and cytologic findings similar to those seen in domestic animals.
Reviewer Acknowledgements
Authors: Evelyn Angelia 
Published: January 2017

Applied Finance and Accounting [AFA] would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether AFA publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 3, Number 1 Abdelaziz Hakimi, University of Jendouba, TunisiaAdam Zaremba, Poznań University of Economics, PolandAnastasia Kopaneli, University of Patras, GreeceAndrey Kudryavtsev, The Max Stern Yezreel Valley Academic College, IsraelArash Riasi, University of Delaware, USAAugustine Akhidime, Benson Idahosa University, NigeriaDesti Kannaiah, James Cook University, SingaporeFeng Jui Hsu, National Taichung Univ. of Sci. & Tech., TaiwanGheorghe Morosan, Stefan Cel Mare Univ. Suceava Romania, RomaniaIoan Bogdan Robu, Alexandru Ioan Cuza University of Iasi, RomaniaJames Estes, California State University San Bernardino, USAKesseven Padachi, University of Technology, MauritiusLasse Oulasvirta, University of Tampere, FinlandLuca Sensini, University of Salerno, ItalyMarco Muscettola, Independent researcher, ItalyMazurina Mohd Ali, Universiti Teknologi Mara, MalaysiaMohamed Jalloh, Eco. Community of West African States, NigeriaMojeed Idowu John Odumeso-Jimoh, Noble Integrated Resources & Management, NigeriaNicoleta Radneantu, Romanian – American University, RomanianNikolay Patonov, European Polytechnical University, BulgariaNoriaki Okamoto, Rikkyo University, JapanPeibiao Zhao, Nanjing University of Science & Technology, ChinaSawsan Saadi Halbouni, Canadian University Dubai, UAEVineet Chouhan, Sir Padampat Singhania University, IndiaWilson E. Herbert, Bingham University, NigeriaYu Peng Lin, University of Detroit Mercy, USA Angelia EvelynEditorial AssistantOn behalf of,The Editorial Board of Applied Finance and AccountingRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAE-mail: afa@redfame.comURL: http://afa.redfame.com
Proteomic and Mutant Analysis of the CO
2
Concentrating Mechanism of Hydrothermal Vent Chemolithoautotroph Thiomicrospira crunogena
Authors: Mangiapia Mary ,   USF MCB4404L, Brown Terry-René W. , Chaput Dale , Haller Edward , Harmer Tara L. , Hashemy Zahra , Keeley Ryan , Leonard Juliana , Mancera Paola , Nicholson David , Stevens Stanley , Wanjugi Pauline , Zabinski Tania , Pan Chongle , Scott Kathleen M. 
Published: January 2017

ABSTRACT

Many autotrophic microorganisms are likely to adapt to scarcity in dissolved inorganic carbon (DIC; CO
2
+ HCO
3
−
+ CO
3
2−
) with CO
2
concentrating mechanisms (CCM) that actively transport DIC across the cell membrane to facilitate carbon fixation. Surprisingly, DIC transport has been well studied among cyanobacteria and microalgae only. The deep-sea vent gammaproteobacterial chemolithoautotroph
Thiomicrospira crunogena
has a low-DIC inducible CCM, though the mechanism for uptake is unclear, as homologs to cyanobacterial transporters are absent. To identify the components of this CCM, proteomes of
T. crunogena
cultivated under low- and high-DIC conditions were compared. Fourteen proteins, including those comprising carboxysomes, were at least 4-fold more abundant under low-DIC conditions. One of these proteins was encoded by
Tcr_0854
; strains carrying mutated copies of this gene, as well as the adjacent
Tcr_0853
, required elevated DIC for growth. Strains carrying mutated copies of
Tcr_0853
and
Tcr_0854
overexpressed carboxysomes and had diminished ability to accumulate intracellular DIC. Based on reverse transcription (RT)-PCR,
Tcr_0853
and
Tcr_0854
were cotranscribed and upregulated under low-DIC conditions. The
Tcr_0853
-encoded protein was predicted to have 13 transmembrane helices. Given the mutant phenotypes described above,
Tcr_0853
and
Tcr_0854
may encode a two-subunit DIC transporter that belongs to a previously undescribed transporter family, though it is widespread among autotrophs from multiple phyla.

IMPORTANCE
DIC uptake and fixation by autotrophs are the primary input of inorganic carbon into the biosphere. The mechanism for dissolved inorganic carbon uptake has been characterized only for cyanobacteria despite the importance of DIC uptake by autotrophic microorganisms from many phyla among the
Bacteria
and
Archaea
. In this work, proteins necessary for dissolved inorganic carbon utilization in the deep-sea vent chemolithoautotroph
T. crunogena
were identified, and two of these may be able to form a novel transporter. Homologs of these proteins are present in 14 phyla in
Bacteria
and also in one phylum of
Archaea
, the
Euryarchaeota
. Many organisms carrying these homologs are autotrophs, suggesting a role in facilitating dissolved inorganic carbon uptake and fixation well beyond the genus
Thiomicrospira
.
An evaluation study of the Becton-Dickinson ProbeTec Qx (BDQx) Trichomonas vaginalis trichomoniasis molecular diagnostic test in two large, urban STD services
Authors: Tipple Craig , Rayment Michael , Mandalia Sundhiya , Walton Lisa , O'Neill Sinead , Murray John , O'Riordan Alan , Rebec Monica , Harrington Laura , Jones Rachael , Wilkinson Dawn 
Published: January 2017

ObjectivesThe BASHH guidelines recommend molecular tests to aid diagnosis of Trichomonas vaginalis (TV) infection; however many clinics continue to use relatively insensitive techniques (pH, wet-prep microscopy (WPM) and culture). Our objectives were to establish a laboratory pathway for TV testing with the Becton-Dickinson Qx (BDQx) molecular assay, to determine TV prevalence and to identify variables associated with TV detection.MethodsA prospective study of 901 women attending two urban sexual health services for STI testing was conducted. Women were offered TV BDQx testing in addition to standard of care. Data collected were demographics, symptoms, results of near-patient tests and BDQx results for TV, Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC). Women with any positive TV result were treated and followed up for test of cure (TOC).Results901 women had a TV BDQx test. 472 (53%) were white, 143 (16%) black and 499 (55%) were symptomatic. Infections detected by BDQx were: 11 TV (1.2%), three GC (0.3%) and 44 CT (4.9%). Of the 11 BDQx-detected TV infections, 8 (73%) were in patients of black ethnicity. Of these, four of seven cases (57%) were WPM-positive. All patients received treatment and nine of nine (100%) were BDQx-negative at TOC. In univariate analysis, only black ethnicity was associated with likelihood of a positive TV BDQx result (relative risk (RR) 10.2 (95% CI 2.15 to 48.4)).ConclusionsThe use of the BDQ enhanced detection of TV in asymptomatic and symptomatic populations. Cost-effective implementation of the test will rely on further work to reliably detect demographic and clinical variables that predict positivity.
RelB Expression Determines the Differential Effects of Ascorbic Acid in Normal and Cancer Cells
Authors: Wei Xiaowei , Xu Yong , Xu Fang Fang , Chaiswing Luksana , Schnell David , Noel Teresa , Wang Chi , Chen Jinfei , St. Clair Daret K. , St. Clair William H. 
Published: January 2017

Abstract
Cancer cells typically experience higher oxidative stress than normal cells, such that elevating pro-oxidant levels can trigger cancer cell death. Although pre-exposure to mild oxidative agents will sensitize cancer cells to radiation, this pre-exposure may also activate the adaptive stress defense system in normal cells. Ascorbic acid is a prototype redox modulator that when infused intravenously appears to kill cancers without injury to normal tissues; however, the mechanisms involved remain elusive. In this study, we show how ascorbic acid kills cancer cells and sensitizes prostate cancer to radiation therapy while also conferring protection upon normal prostate epithelial cells against radiation-induced injury. We found that the NF-κB transcription factor RelB is a pivotal determinant in the differential radiosensitization effects of ascorbic acid in prostate cancer cells and normal prostate epithelial cells. Mechanistically, high reactive oxygen species concentrations suppress RelB in cancer cells. RelB suppression decreases expression of the sirtuin SIRT3 and the powerful antioxidant MnSOD, which in turn increases oxidative and metabolic stresses in prostate cancer cells. In contrast, ascorbic acid enhances RelB expression in normal cells, improving antioxidant and metabolic defenses against radiation injury. In addition to showing how RelB mediates the differential effects of ascorbic acid on cancer and normal tissue radiosensitivities, our work also provides a proof of concept for the existence of redox modulators that can improve the efficacy of radiotherapy while protecting against normal tissue injury in cancer settings. Cancer Res; 77(6); 1345–56. ©2017 AACR.
FLT3 and JAK2 Mutations in Acute Myeloid Leukemia Promote Interchromosomal Homologous Recombination and the Potential for Copy Neutral Loss of Heterozygosity
Authors: Gaymes Terry J. , Mohamedali Azim , Eiliazadeh Anthony L. , Darling David , Mufti Ghulam J. 
Published: January 2017

Abstract
Acquired copy neutral LOH (CN-LOH) is a frequent occurrence in myeloid malignancies and is often associated with resistance to standard therapeutic modalities and poor survival. Here, we show that constitutive signaling driven by mutated FLT3 and JAK2 confers interchromosomal homologous recombination (iHR), a precedent for CN-LOH. Using a targeted recombination assay, we determined significant iHR activity in internal tandem duplication FLT3 (FLT3-ITD) and JAK2V617F-mutated cells. Sister chromatid exchanges, a surrogate measure of iHR, was significantly elevated in primary FLT3-ITD normal karyotype acute myeloid leukemia (NK-AML) compared with wild-type FLT3 NK-AML. HR was harmonized to S phase of the cell cycle to repair broken chromatids and prevent iHR. Increased HR activity in G0 arrested primary FLT3-ITD NK-AML in contrast to wild-type FLT3 NK-AML. Cells expressing mutated FLT3-ITD demonstrated a relative increase in mutation frequency as detected by thymidine kinase (TK) gene mutation assay. Moreover, resistance was associated with CN-LOH at the TK locus. Treatment of FLT3-ITD– and JAK2V617F-mutant cells with the antioxidant N-acetylcysteine diminished reactive oxygen species (ROS), restoring iHR and HR levels. Our findings show that mutated FLT3-ITD and JAK2 augment ROS production and HR, shifting the cellular milieu toward illegitimate recombination events such as iHR and CN-LOH. Therapeutic reduction of ROS may thus prevent leukemic progression and relapse in myeloid malignancies. Cancer Res; 77(7); 1697–708. ©2017 AACR.
A Genome-Wide Loss-of-Function Screen Identifies SLC26A2 as a Novel Mediator of TRAIL Resistance
Authors: Dimberg Lina Y. , Towers Christina G. , Behbakht Kian , Hotz Taylor J. , Kim Jihye , Fosmire Susan , Porter Christopher C. , Tan Aik-Choon , Thorburn Andrew , Ford Heide L. 
Published: January 2017

Abstract
TRAIL is a potent death-inducing ligand that mediates apoptosis through the extrinsic pathway and serves as an important endogenous tumor suppressor mechanism. Because tumor cells are often killed by TRAIL and normal cells are not, drugs that activate the TRAIL pathway have been thought to have potential clinical value. However, to date, most TRAIL-related clinical trials have largely failed due to the tumor cells having intrinsic or acquired resistance to TRAIL-induced apoptosis. Previous studies to identify resistance mechanisms have focused on targeted analysis of the canonical apoptosis pathway and other known regulators of TRAIL receptor signaling. To identify novel mechanisms of TRAIL resistance in an unbiased way, we performed a genome-wide shRNA screen for genes that regulate TRAIL sensitivity in sublines that had been selected for acquired TRAIL resistance. This screen identified previously unknown mediators of TRAIL resistance including angiotensin II receptor 2, Crk-like protein, T-Box Transcription Factor 2, and solute carrier family 26 member 2 (SLC26A2). SLC26A2 downregulates the TRAIL receptors, DR4 and DR5, and this downregulation is associated with resistance to TRAIL. Its expression is high in numerous tumor types compared with normal cells, and in breast cancer, SLC26A2 is associated with a significant decrease in relapse-free survival.
Implication: Our results shed light on novel resistance mechanisms that could affect the efficacy of TRAIL agonist therapies and highlight the possibility of using these proteins as biomarkers to identify TRAIL-resistant tumors, or as potential therapeutic targets in combination with TRAIL. Mol Cancer Res; 15(4); 382–94. ©2017 AACR.
Oncogenic KRAS Targets MUC16/CA125 in Pancreatic Ductal Adenocarcinoma
Authors: Liang Chen , Qin Yi , Zhang Bo , Ji Shunrong , Shi Si , Xu Wenyan , Liu Jiang , Xiang Jinfeng , Liang Dingkong , Hu Qiangsheng , Ni Quanxing , Xu Jin , Yu Xianjun 
Published: January 2017

Abstract
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with the 5-year survival rate less than 6%. Previous results indicated that serum levels of CA125 (encoded by MUC16) could be used to predict which groups of pancreatic cancer patients may benefit from surgery. However, the underlying mechanism remains elusive. Herein, using the Cancer Genome Atlas and clinicopathologic data obtained from our center, we demonstrate that high CA125 serum levels and expression levels of MUC16 are predictive of poor prognosis. MUC16 is also validated as a downstream target of KRAS, and their expression strongly correlated with each other in vitro and in vivo. Mechanistically, the KRAS/ERK axis induced upregulation of MUC16 and shedding of CA125 via its effector c-Myc in SW1990 and PANC-1 pancreatic cancer cells. Notably, proto-oncogene c-Myc could bind to the promoter of MUC16 and transcriptionally activate its expression. Taken together, these data establish CA125 as a prognostic marker for pancreatic cancer, and mechanistic studies uncovered the KRAS/c-Myc axis as a driving factor for upregulation of MUC16.
Implications: The current study uncovers the contribution of oncogenic KRAS to serum marker CA125 production through a mechanism that involves the ERK/c-Myc axis. Mol Cancer Res; 15(2); 201–12. ©2016 AACR.