bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field-Evolved Mode 1 Resistance of the Fall Armyworm to Transgenic Cry1Fa-Expressing Corn Associated with Reduced Cry1Fa Toxin Binding and Midgut Alkaline Phosphatase Expression
Authors: Jakka Siva R. K. , Gong Liang , Hasler James , Banerjee Rahul , Sheets Joel J. , Narva Kenneth , Blanco Carlos A. , Jurat-Fuentes Juan L. 
Published: December 2015

ABSTRACT

Insecticidal protein genes from the bacterium
Bacillus thuringiensis
(Bt) are expressed by transgenic Bt crops (Bt crops) for effective and environmentally safe pest control. The development of resistance to these insecticidal proteins is considered the most serious threat to the sustainability of Bt crops. Resistance in fall armyworm (
Spodoptera frugiperda
) populations from Puerto Rico to transgenic corn producing the Cry1Fa insecticidal protein resulted, for the first time in the United States, in practical resistance, and Bt corn was withdrawn from the local market. In this study, we used a field-collected Cry1Fa corn-resistant strain (456) of
S. frugiperda
to identify the mechanism responsible for field-evolved resistance. Binding assays detected reduced Cry1Fa, Cry1Ab, and Cry1Ac but not Cry1Ca toxin binding to midgut brush border membrane vesicles (BBMV) from the larvae of strain 456 compared to that from the larvae of a susceptible (Ben) strain. This binding phenotype is descriptive of the mode 1 type of resistance to Bt toxins. A comparison of the transcript levels for putative Cry1 toxin receptor genes identified a significant downregulation (>90%) of a membrane-bound alkaline phosphatase (ALP), which translated to reduced ALP protein levels and a 75% reduction in ALP activity in BBMV from 456 compared to that of Ben larvae. We cloned and heterologously expressed this ALP from susceptible
S. frugiperda
larvae and demonstrated that it specifically binds with Cry1Fa toxin. This study provides a thorough mechanistic description of field-evolved resistance to a transgenic Bt crop and supports an association between resistance and reduced Cry1Fa toxin binding and levels of a putative Cry1Fa toxin receptor, ALP, in the midguts of
S. frugiperda
larvae.
Comparative Evaluation of the Diagnostic Performance of the Prototype Cepheid GeneXpert Ebola Assay
Authors: Jansen van Vuren Petrus , Grobbelaar Antoinette , Storm Nadia , Conteh Ousman , Konneh Kelfala , Kamara Abdul , Sanne Ian , Paweska Janusz T. 
Published: December 2015

ABSTRACT

The Ebola virus disease (EVD) outbreak in West Africa has highlighted an urgent need for point-of-care (POC) assays for the diagnosis of this devastating disease in resource-limited African countries. The diagnostic performance characteristics of a prototype Cepheid GeneXpert Ebola POC used to detect Ebola virus (EBOV) in stored serum and plasma samples collected from suspected EVD cases in Sierra Leone in 2014 and 2015 was evaluated. The GeneXpert Ebola POC is a self-contained single-cartridge automated system that targets the glycoprotein (GP) and nucleoprotein (NP) genes of EBOV and yields results within 90 min. Results from 281 patient samples were compared to the results of a TaqMan real-time reverse transcription-PCR (RT-PCR) targeting the polymerase gene and performed on two real-time PCR machines. Agreement between the three platforms was 100% at cycle threshold (

C
T

) values of ≤34.99, but discordant results were noted between

C
T

values of 35 and 45.The diagnostic sensitivity of the three platforms was 100% in 91 patient samples that were confirmed to be infectious by virus isolation. All three molecular platforms detected viral EBOV RNA in additional samples that did not contain viable EBOV. The analytical sensitivity of the GeneXpert Ebola POC for the detection of NP was higher, and comparable to that of polymerase gene detection, than that for the detection of GP when using a titrated laboratory stock of EBOV. There was no detectable cross-reactivity with other hemorrhagic fever viruses or arboviruses. The GeneXpert Ebola POC offers an easy to operate and sensitive diagnostic tool that can be used for the rapid screening of suspected EVD cases in treatment or in holding centers during EVD outbreaks.
Age-Related Risk Factors for Revision Anterior Cruciate Ligament Reconstruction
Authors: Maletis Gregory B. , Chen Jason , Inacio Maria C.S. , Funahashi Tadashi T. 
Published: December 2015

Background: It is not clear whether risk factors for revision anterior cruciate ligament reconstruction (ACLR) are the same throughout patients’ lives. Purpose: To assess (1) the risk of revision ACLR by age and (2) age-specific risk factors for revision ACLR. Study Design: Cohort study; Level of evidence, 2. Methods: A cohort study using patients having undergone primary ACLR who were registered in the Kaiser Permanente ACLR Registry (February 2005 to June 2013) was conducted. Aseptic revision was the main endpoint. Age was evaluated as a risk factor for revision. The cohort was stratified into 4 age groups: <21, 21-30, 31-40, and >40 years. Graft type, sex, body mass index (BMI), and race were assessed as revision risk factors within each group. Survival analyses were conducted. Results: Of the 21,304 patients evaluated, 7026 (33%) patients were aged <21 years, 5762 (27%) were 21-30 years, 4656 (22%) were 31-40 years, and 3860 (18%) were >40 years. Allografts were used in 8671 (41%) patients, hamstring autografts in 6823 (32%), and bone–patellar tendon–bone (BPTB) autografts in 5260 (25%). The 5-year revision probability was highest in patients <21 years old (9.0%; 95% CI, 8.0%-10.1%) and lowest in those >40 years old (1.9%; 95% CI, 1.3%-2.7%). Compared with patients aged >40 years, the adjusted revision risk for patients aged <21 years was 7.76 (95% CI, 5.52-10.90). In patients aged <21 years, a lower revision risk was seen in female patients (hazard ratio [HR], 0.76; 95% CI, 0.61-0.93) compared with male patients, patients with a BMI of 30-35 kg/m2 (HR, 0.75; 95% CI, 0.59-0.95) and BMI >35 kg/m2 (HR, 0.49; 95% CI, 0.34-0.70) compared with those with a BMI <30 kg/m2, and black patients (HR, 0.55, 95% CI, 0.36-0.85) compared with white patients. Sex, BMI, and race were not associated with the risk of revision in older patients. In patients ≤40 years old, those with allografts had a higher risk of revision than those with BPTB autografts (HR, 2.69, 2.35, and 3.04 for patients aged <21, 21-30, and 31-40 years, respectively). Patients <21 years old with hamstring autografts had a 1.61 times (95% CI, 1.20-2.17) higher risk of revision than did patients with BPTB autografts; these differences were not identified in older patients. Conclusion: Age at the time of ACLR surgery is a strong risk factor for revision ACLR. Other risk factors for revision ACLR, such as graft type, sex, race, and BMI, may vary in strength and significance over a patient’s life. Understanding the age-related risk factors associated with revision ACLR may help with appropriate patient counseling and optimal graft choice when performing ACLR.
Endovascular treatment of distal middle cerebral artery aneurysms: Report of eight cases and literature review
Authors: Lv Nan , Zhou Yu , Yang Pengfei , Li Qiang , Zhao Rui , Fang Yibin , Xu Yi , Hong Bo , Zhao Wenyuan , Liu Jianmin , Huang Qinghai 
Published: December 2015

Background Endovascular treatment is an alternative choice for the treatment of distal middle cerebral artery (dMCA) aneurysm, in addition to open surgery; but is still seldom considered. We performed this retrospective study to evaluate the outcome of dMCA in patients. Methods During a period of 10 years, we were able to identify seven patients with a total of eight dMCA aneurysms that were treated endovascularly. They were five men and two women, with a mean age of 36.1 years. All of the aneurysms, including five infectious and three dissecting ones, were treated for the aneurysm and its parent artery’s occlusion, using coils and/or glue. Results The clinical follow-up (9–96 m, mean 36.8 m) showed that they all improved over baseline; except for one patient in whom a mild right hemiparesis remained, after the hematoma evacuation. Angiographic follow-up (7–24 m; mean: 14.6 m) showed that all of them were stable and without the need for recanalization. Conclusions Our data indicated that endovascular treatment is a safe and effective alternative for the treatment of dMCA aneurysms, and should be considered when treating these aneurysms.
Comparison of clinical outcome with radiological findings after trapeziectomy with ligament reconstruction and tendon interposition
Authors: Reissner L. , Marks M. , Schindele S. , Herren D. B. 
Published: December 2015

The aim of our study was to investigate if pre- and postoperative radiographic thumb carpometacarpal position is correlated with clinical and subjective outcomes. Radiographs of 105 patients undergoing trapeziectomy with ligament reconstruction and tendon interposition were analysed before, as well as 1 year after, surgery for dorsal subluxation and proximal migration of the thumb metacarpal bone. Furthermore, key pinch strength was measured and patients completed the Michigan Hand Outcomes Questionnaire. Baseline dorsal subluxation and scaphometacarpal distances significantly decreased from 8.2 mm and 11.0 mm to 5.2 mm and 5.1 mm at 1 year, respectively. There was no correlation between the amount of subluxation or proximal migration and the Michigan Hand Outcomes Questionnaire score or key pinch strength. These results suggest that postoperative position of the metacarpal base of the thumb does not affect clinical or subjective outcomes after trapeziectomy with ligament reconstruction and tendon reposition of the thumb carpometacarpal joint. Level of evidence: Level 3
Crystal Structure of 4-Methylbenzenecarbothioic Acid and Computational Investigations of Benzenecarbochalcogenoic Acids (C6H5COEH and C6H5CEOH, E = S, Se, Te)
Authors: Kato Shinzi , Niyomura Osamu , Ebihara Masahiro , Guo Jing-Dong , Nagase Shigeru 
Published: December 2015

Abstract
The X-ray structure analysis of arenecarbochalcogenoic acids (ArCOEH and ArCEOH, E = S, Se, Te) is successful for 4-MeC6H4COSH for the first time, which reveals that the syn-conformer forms a planar cyclic dimer with two S–H···O=C hydrogen bonds in the solid state. Density functional theory calculations are performed for benzenecarbochalcogenoic acids (C6H5COEH and C6H5CEOH), the tetrahydrofuran (THF)-solvated acids, and the cyclic dimers of the acids. It is suggested that arenechalcogenol acids (ArCOEH) form cyclic dimers in the solid state, although Della Védova and co-workers reported that catemeric hydrogen-bonding chains are observed for the crystal structures of selenoacetic acid (MeCOSeH). Calculations demonstrate that arenecarbochalcogenoic acids exist predominantly as chalcogenoxo tautomers (ArCEOH) in polar solvents such as THF especially at low temperatures, unlike the solid-state cases, which are consistent with several experimental results.
Composition and Genesis of the Nickel-Chrome-Bearing Nontronite and Montmorillonite in Lateritized Ultramafic Rocks in the Muratdği Region (Uşak, Western Anatolia), Turkey
Authors: Kadır Selahattın , Aydoğan M. Selman , Elıtok Ömer , Helvaci Cahıt 
Published: December 2015

AbstractWidespread lateritized ultramafic rocks in the southern part of the Muratdağı region of Turkey constitute a significant source of Ni-Cr-bearing ore with economic potential. However, no mineralogical or geochemical characterizations of these important materials have been performed previously. The aim of the present study was to describe the mineralogy, geochemistry, and genesis of Ni-Cr-bearing smectite in garnierite and ferruginous saprolite associated with the lateritized ophiolite-related ultramafic rocks. The lateritic zones are well developed over serpentinized harzburgitic mantle peridotites. The lateritized units and related bedrocks were examined using polarized-light microscopy, X-ray diffraction, scanning and transmission electron microscopies, and chemical and isotopic methods. The garnierite-containing saprolites are enriched in smectite, Fe-(oxyhydr)oxide phases, and opal-CT. Micromorphological images revealed that flaky smectite and, locally, Fe-rich particles, alunite, gypsum, gibbsite, and sulfur crystals developed along the fractures and dissolution voids. The development of saprolite demonstrates chemical weathering. The presence of silicified and Fe-(oxyhydr)oxide phases associated with gypsum, alunite, and local native sulfur in vertical and/or subvertical fractures and fault infillings are indicative of hydrothermal processes along the extensional, tectonically related fault systems. Chemical weathering and hydrothermal processes, which probably started during the Oligocene and Miocene, led to the formation of nontronite, Fe-bearing montmorillonite, and local Fe-rich kaolinite. Nickel and Cr are concentrated significantly in the saprolite zone and are positively correlated with Fe2O3 content, which is controlled by the formation of nontronite, montmorillonite, and Fe-(oxyhydr)oxide phases. Nickel-Cr-bearing nontronite and montmorillonite precipitated from alkaline water as a result of the increasing (Fe2O3+Al2O3+Cr2O5+Ni+Co)/(MgO+SiO2) ratio under the control of both chemical weathering and hydrothermal processes. The Fe and Mg (associated with Ni and Cr) required for the formation of smectite were supplied by solutions from both chemical weathering and hydrothermal alteration of Ni-Cr-bearing olivine and pyroxene in the harzburgitic bedrock; the Al was supplied by schists, granite, and volcanic units.
Functional Studies on Viable Circulating Tumor Cells
Authors: Pantel Klaus , Alix-Panabières Catherine 
Published: December 2015

AbstractBACKGROUNDResearch on circulating tumor cells (CTCs) as new biomarkers has received great attention over the past decade. In particular, the capture and analysis of CTCs as “liquid biopsies” provides the possibility to avoid invasive tissue biopsies, with obvious implications in cancer diagnostics.CONTENTThe focus of this review is to describe and discuss how functional studies on viable CTCs can enlarge the spectrum of applications of liquid biopsies, with emphasis on breast, prostate, colon, and lung cancer as the major tumor entities in industrialized countries. The low number of CTCs in the peripheral blood of most cancer patients makes challenging the in vitro culture of CTCs. Epithelial tumor cells are difficult to culture, even when starting with millions of tumor cells. Recently, several groups have achieved important advances in the in vitro and in vivo expansion of CTCs from cancer patients at very advanced stages with higher amounts of CTCs. Here, we present current technologies to enrich and detect viable human CTCs, including positive and negative enrichment strategies that are based on antigen expression and physical properties of CTCs. We also discuss published data about functional studies on CTCs that use in vitro and in vivo models.SUMMARYFunctional analyses on CTCs offer the possibility to identify the biological properties of metastatic cells, including the identification of metastasis-initiating cells. Moreover, CTC-derived cell lines and xenografts might reveal new therapeutic targets and can be used for drug screening.
Documenting migrant labour in Moscow’s Teatr.doc
Authors: Aizman Ania 
Published: December 2015

Abstract
In Russia, documentary theatre can be seen as a challenge to a bureaucratic logic that was famously summarized in Mikhail Bulgakov’s novel The Master and Margarita: “If there are no papers, there is no person.” Teatr.doc, the first documentary theatre to open in Moscow, has recorded a “human document” for undocumented immigrants by staging plays about the lives of ethnic minorities in Moscow. Recently threatened with the loss of its 12-year-old basement space, Teatr.doc must be studied as an institution that created space for public discussion despite increasing state supervision of culture. This paper describes Teatr.doc’s efforts to document the everyday lives of undocumented labourers living amidst ethnic stensions in Moscow.
Historically speaking: The workings of the past in a performance by Olof Olsson
Authors: Sørensen Trine Friis 
Published: December 2015

Abstract
This article sets out to untangle the workings of the past in a performance by Danish-Swedish-Dutch artist Olof Olsson. Leaving behind the official documents of the Danmarks Radio (DR) Archive, Olsson explores the byroads of radio history through a miscellaneous assortment of nugatory documents and objects. The article proceeds as a close reading of aspects of Olsson’s performance using three figures described by Walter Benjamin – the collector, the storyteller and the historian – as points of reference. By tracing out the contours of these figures in Olsson’s performance, I am able to elucidate the subversive potentiality of his digressive narrative.
Documentary = arbitrary: A few practical-theoretical impulses
Authors: Dobovšek Zala 
Published: December 2015

Abstract
The 2014 Mladi levi international festival directed its overall programme scheme towards documentary and participatory performances, and it also offered a workshop on documentary theatre. The formats of documentary and participatory theatre (with all their variants) don’t just trace the artistic maturity of a certain environment in itself but are always also indices showing the broader picture of the social (political, cultural, etc.) and thereby mental situation of a certain space and time. This contribution combines theory and practice; it considers documentary theatre in various theoretical frameworks, compares its various staging approaches (personal memory, collective memory, simulations, reconstructions) and offers a brief insight into the creation and the procedures of this genre.
Waste management: Self interview on the Temporary Slovenian Dance Archive
Authors: Vevar Rok 
Published: December 2015

Abstract
The contribution takes the form of a self-interview to tackle the analysis of the historicization of contemporary performing arts on the illustrative example of the Temporary Slovenian Dance Archive, founded by the author, a “precarious cultural worker”, as his personal cultural-activist project. The contribution offers basic information on the scale of the material, its systematization and the archive’s operating principle, which for now runs with no promise of adequate public infrastructure. The self-interview auto-reflexively unfolds the complex issue of the Temporary Slovenian Dance Archive, which stems from a wider issue of historicizing elusive, procedural and performative contemporary performing arts; at the same time, the self-interview begins to tackle deeper reasons for the lack of institutional background, which discourages the Temporary Slovenian Dance Archive from hoping to ever have any stable and continuous function. Most of all, the self-interview on the Temporary Slovenian Dance Archive opens up a question that exceeds contemporary performing arts, namely, in what way can the “impossible history” of non-territorial and transnational character be historicized at all.
Performed by ecologies: How Homo sapiens could subvert present-day futures
Authors: Kershaw Baz 
Published: December 2015

Abstract
There is little doubt that humankind faces an environmental crisis in which the dinosaurs’ extinction will look like a children’s tea party staged by drunken clowns. But still the vast majority – at least in so-called developed countries – behave as if that prospect is a chimera. Globally such denial is now ecologically systemic, and so I call that behaviour ‘performance compulsion’. This conundrum is explored below through an elementary conceit. While humans assume that they possess agencies unique among the species, we are fundamentally performed by Earth’s ecologies. Hence, the only hope of averting our extinction is to perform more responsively and ethically with those ecologies.
Reviews
Authors: Ames Margaret , Bottoms Stephen , Franks Aaron , Magnat Virginie , Weigler Will 
Published: December 2015

Abstract
Disability, Public Space Performance and Spectatorship: Unconscious Performers, Bree Hadley (2014) New York: Palgrave Macmillan, 224 pp., ISBN: 9781137396075
Enacting Nature: Ecocritical Perspectives On Indigenous Performance, Birgit Däwes and Marc Maufort (eds) (2014) Brussels: Peter Lang (2014), 262 pp. ISBN-10: 2875741462; ISBN-13: 978-2875741462
Playing for Time: Making Art as if the World Mattered, Lucy Neal (ed.) (2015) Oberon Books, London, 466 pp., ISBN: 9781783191864, p/bk, 16.99 GBP
Performing Indigeneity: Global Histories and Contemporary Experiences, Laura R. Graham and H. Glenn Penny (eds) (2014) Lincoln and London: University of Nebraska Press, 431 pages ISBN 978-0-8032-5686-6 (paperback), $36 USD
Salmon is Everything: Community-based Theatre in the Klamath Watershed, Theresa May, Suzanne Burcell, Kathleen Mccovey and Jean O’Hara (2014) Corvallis: Oregon State University Press, 188 pp., ISBN: 9780870717468, p/bk, $19.95
Hope is a Wooded Time: An eco-performance of biodiversity in discarded geographic and social space
Authors: Haedicke susan , Harper Sarah 
Published: December 2015

Abstract
Friches Théâtre Urbain’s Hope is a Wooded Time is an ongoing community-based eco-art project outside Paris that draws inspiration from its site’s evocative heritage as part of les Murs à Pêches, ‘living walls’ for espaliered fruit trees in the seventeenth to nineteenth centuries, and its contemporary reality as a wooded wasteland. The project encourages ethical encounters between ecological processes of biodiversity and human interventions by its neighbours, ethnically and culturally diverse populations of Sinti and Romany gypsies, Russian and North African immigrants and native French.
Locating an Indigenous ethos in ecological performance
Authors: Woynarski Lisa 
Published: December 2015

Abstract
Stories are powerful forms of representation and cultural imagery in many Indigenous cultures, and performance is a site where these stories are shared, revealed and enacted, making it a powerful site of cultural imagery for Indigenous ecological knowledges and cosmologies. I argue that an Indigenous ecological ethos is a necessary addition to thinking about performance and ecology, one that resists patronizing and simplistic stereotypes of the ‘eco-Indian’ and acknowledges diverse, complex and evolving epistemologies. Drawing on Huggan and Tiffin’s postcolonialism ecocriticism, as well as May and Kuppers experience as non-Indigenous scholars and practitioners, this article considers the role postcolonial ecology might play in the field of performance and ecology and how non-indigenous scholars and theatremakers might engage with it. I suggest strategies for locating an Indigenous ecological ethos, through Däwes, Nolan, Howe and Halba and their critical reflections on Indigenous performances specifically attuned to ecological concerns. I draw on plays and performances that highlight the inseparability of land, identity and morethan- human (Salmon is Everything, NK603: Action for Performer & e-Maiz, Woman for Walking); work that is non-linear and recognizes the simultaneity of past, present and future (Burning Vision, Chasing Honey); and work that takes up ecological justice issues (Sila). These aspects suggest ways of locating an Indigenous ecological ethos and developing a more multivocal and inclusive field of performance and ecology.
Performing from Heidegger’s Turning
Authors: Grant Stuart 
Published: December 2015

Abstract
This article aims to do two things. (1) Describe aspects of the performance methodology and philosophy of the site-specific ecological performance group, the Environmental Performance Authority (EPA), in a series of performances entitled Coastal Scales, developed in conjunction with the Hobsons Bay Council in Melbourne, Australia. (2) Make an argument for the efficacy of the EPA’s work, through concepts from Heidegger’s later writings, with particular reference to the ‘turning’, and the occurrence of truth as the strife between earth and world, as developed in Contributions to Philosophy (1999, 2012) and ‘The origin of the work of art’ (1977a).