bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties
Authors: Canciello Angelo , Russo Valentina , Berardinelli Paolo , Bernabò Nicola , Muttini Aurelio , Mattioli Mauro , Barboni Barbara 
Published: June 2017

AbstractThe in vitro expansion is detrimental to therapeutic applications of amniotic epithelial cells (AEC), an emerging source of fetal stem cells. This study provides molecular evidences of progesterone (P4) role in preventing epithelial-mesenchymal transition (EMT) in ovine AEC (oAEC). oAEC amplified under standard conditions spontaneously acquired mesenchymal properties through the up-regulation of EMT-transcription factors. P4 supplementation prevented phenotype shift by inhibiting the EMT-inducing mechanism such as the autocrine production of TGF-β and the activation of intracellular-related signaling. The effect of P4 still persisted for one passage after steroid removal from culture as well as steroid supplementation promptly reversed mesenchymal phenotype in oAEC which have experienced EMT during amplification. Furthermore, P4 promoted an acute up-regulation of pluripotent genes whereas enhanced basal and LPS-induced oAEC anti-inflammatory response with an increase in anti-inflammatory and a decrease in pro-inflammatory cytokines expression. Altogether, these results indicate that P4 supplementation is crucial to preserve epithelial phenotype and to enhance biological properties in expanded oAEC. Therefore, an innovative cultural approach is proposed in order to improve therapeutic potential of this promising source of epithelial stem cells.
Colony adaptive response to simulated heat waves and consequences at the individual level in honeybees (Apis mellifera)
Authors: Bordier Célia , Dechatre Hélène , Suchail Séverine , Peruzzi Mathilde , Soubeyrand Samuel , Pioz Maryline , Pélissier Michel , Crauser Didier , Conte Yves Le , Alaux Cédric 
Published: June 2017

AbstractSince climate change is expected to bring more severe and frequent extreme weather events such as heat waves, assessing the physiological and behavioural sensitivity of organisms to temperature becomes a priority. We therefore investigated the responses of honeybees, an important insect pollinator, to simulated heat waves (SHW). Honeybees are known to maintain strict brood thermoregulation, but the consequences at the colony and individual levels remain poorly understood. For the first time, we quantified and modelled colony real-time activity and found a 70% increase in foraging activity with SHW, which was likely due to the recruitment of previously inactive bees. Pollen and nectar foraging was not impacted, but an increase in water foragers was observed at the expense of empty bees. Contrary to individual energetic resources, vitellogenin levels increased with SHW, probably to protect bees against oxidative stress. Finally, though immune functions were not altered, we observed a significant decrease in deformed wing virus loads with SHW. In conclusion, we demonstrated that honeybees could remarkably adapt to heat waves without a cost at the individual level and on resource flow. However, the recruitment of backup foraging forces might be costly by lowering the colony buffering capacity against additional environmental pressures.
Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli
Authors: Kulmala Antti , Huovinen Tuomas , Lamminmäki Urpo 
Published: June 2017

AbstractCodon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one codon” method. We redesigned five segments of the Fab gene with a “codon harmonization” method described by Angov et al. and studied the effects of these changes on cell viability, Fab yield and display on filamentous phage using different vectors and bacterial strains. The harmonization considerably reduced toxicity, increased Fab expression from negligible levels to 10 mg/l, and restored the display on phage. Testing the impact of the individual redesigned segments revealed that the most significant effects were conferred by changes in the constant domain of the light chain. For some of the Fab gene variants, we also observed striking differences in protein yields when cloned from a chloramphenicol resistant vector into an identical vector, except with ampicillin resistance. In conclusion, our results show that the expression of a heterodimeric secretory protein can be improved by harmonizing selected DNA segments by synonymous codons and reveal additional complexity involved in heterologous protein expression.
Dietary mastic oil extracted from Pistacia lentiscus var. chia suppresses tumor growth in experimental colon cancer models
Authors: Spyridopoulou Katerina , Tiptiri-Kourpeti Angeliki , Lampri Evangeli , Fitsiou Eleni , Vasileiadis Stavros , Vamvakias Manolis , Bardouki Haido , Goussia Anna , Malamou-Mitsi Vasiliki , Panayiotidis Mihalis I. , Galanis Alex , Pappa Aglaia , Chlichlia Katerina 
Published: June 2017

AbstractPlant-derived bioactive compounds attract considerable interest as potential chemopreventive anticancer agents. We analyzed the volatile dietary phytochemicals (terpenes) present in mastic oil extracted from the resin of Pistacia lentiscus var. chia and comparatively investigated their effects on colon carcinoma proliferation, a) in vitro against colon cancer cell lines and b) in vivo on tumor growth in mice following oral administration. Mastic oil inhibited - more effectively than its major constituents- proliferation of colon cancer cells in vitro, attenuated migration and downregulated transcriptional expression of survivin (BIRC5a). When administered orally, mastic oil inhibited the growth of colon carcinoma tumors in mice. A reduced expression of Ki-67 and survivin in tumor tissues accompanied the observed effects. Notably, only mastic oil -which is comprised of 67.7% α-pinene and 18.8% myrcene- induced a statistically significant anti-tumor effect in mice but not α-pinene, myrcene or a combination thereof. Thus, mastic oil, as a combination of terpenes, exerts growth inhibitory effects against colon carcinoma, suggesting a nutraceutical potential in the fight against colon cancer. To our knowledge, this is the first report showing that orally administered mastic oil induces tumor-suppressing effects against experimental colon cancer.
HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death
Authors: Okamoto Akihisa , Sumi Chisato , Tanaka Hiromasa , Kusunoki Munenori , Iwai Teppei , Nishi Kenichiro , Matsuo Yoshiyuki , Harada Hiroshi , Takenaga Keizo , Bono Hidemasa , Hirota Kiichi 
Published: June 2017

AbstractThe local anesthetic lidocaine induces cell death by altering reactive oxygen species (ROS) generation and mitochondrial electron transport chain function. Because hypoxia-inducible factor 1 (HIF-1) is involved in determining oxygen metabolism and mitochondria function, we investigated the involvement of HIF-1 activity in lidocaine-induced cell death. We investigated the role of HIF activation on lidocaine-induced caspase activation and cell death in renal cell-derived RCC4 cells lacking functional von Hippel-Lindau (VHL) protein. We demonstrate that HIF-1 suppressed oxygen consumption and facilitated glycolysis in a pyruvate dehydrogenase kinase-1-dependent manner and that activation of HIF-1 conferred resistance to lidocaine-induced cell death. We also demonstrated that exogenous HIF-1 activation, through HIFα-hydroxylase inhibition or exposure to hypoxic conditions, alleviates lidocaine toxicity by suppressing mitochondria function and generating ROS, not only in RCC4 cells, but also in the neuronal SH-SY5Y cells. In conclusion, we demonstrate that HIF-1 activation due to VHL deletion, treatment with small molecule HIFα-hydroxylase inhibitors, and exposure to hypoxic conditions suppresses mitochondrial respiratory chain function and confers resistance to lidocaine toxicity.
Assessment of the role of intracranial hypertension and stress on hippocampal cell apoptosis and hypothalamic-pituitary dysfunction after TBI
Authors: Tan Huajun , Yang Weijian , Wu Chenggang , Liu Baolong , Lu Hao , Wang Hong , Yan Hua 
Published: June 2017

AbstractIn recent years, hypopituitarism caused by traumatic brain injury (TBI) has been explored in many clinical studies; however, few studies have focused on intracranial hypertension and stress caused by TBI. In this study, an intracranial hypertension model, with epidural hematoma as the cause, was used to explore the physiopathological and neuroendocrine changes in the hypothalamic–pituitary axis and hippocampus. The results demonstrated that intracranial hypertension increased the apoptosis rate, caspase-3 levels and proliferating cell nuclear antigen (PCNA) in the hippocampus, hypothalamus, pituitary gland and showed a consistent rate of apoptosis within each group. The apoptosis rates of hippocampus, hypothalamus and pituitary gland were further increased when intracranial pressure (ICP) at 24 hour (h) were still increased. The change rates of apoptosis in hypothalamus and pituitary gland were significantly higher than hippocampus. Moreover, the stress caused by surgery may be a crucial factor in apoptosis. To confirm stress leads to apoptosis in the hypothalamus and pituitary gland, we used rabbits to establish a standard stress model. The results confirmed that stress leads to apoptosis of neuroendocrine cells in the hypothalamus and pituitary gland, moreover, the higher the stress intensity, the higher the apoptosis rate in the hypothalamus and pituitary gland.
Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number
Authors: Cui Fa , Zhang Na , Fan Xiao-li , Zhang Wei , Zhao Chun-hua , Yang Li-juan , Pan Rui-qing , Chen Mei , Han Jie , Zhao Xue-qiang , Ji Jun , Tong Yi-ping , Zhang Hong-xia , Jia Ji-zeng , Zhao Guang-yao , Li Jun-ming 
Published: June 2017

AbstractIn crop plants, a high-density genetic linkage map is essential for both genetic and genomic researches. The complexity and the large size of wheat genome have hampered the acquisition of a high-resolution genetic map. In this study, we report a high-density genetic map based on an individual mapping population using the Affymetrix Wheat660K single-nucleotide polymorphism (SNP) array as a probe in hexaploid wheat. The resultant genetic map consisted of 119 566 loci spanning 4424.4 cM, and 119 001 of those loci were SNP markers. This genetic map showed good collinearity with the 90 K and 820 K consensus genetic maps and was also in accordance with the recently released wheat whole genome assembly. The high-density wheat genetic map will provide a major resource for future genetic and genomic research in wheat. Moreover, a comparative genomics analysis among gramineous plant genomes was conducted based on the high-density wheat genetic map, providing an overview of the structural relationships among theses gramineous plant genomes. A major stable quantitative trait locus (QTL) for kernel number per spike was characterized, providing a solid foundation for the future high-resolution mapping and map-based cloning of the targeted QTL.
Simulated responses of permafrost distribution to climate change on the Qinghai–Tibet Plateau
Authors: Lu Qing , Zhao Dongsheng , Wu Shaohong 
Published: June 2017

AbstractClimate warming causes changes in permafrost distribution, which affects the surface energy balance, hydrologic cycle and carbon flux in cold regions. In this study, the Surface Frost Number model was applied to examine permafrost distribution on the Qinghai–Tibet Plateau (QTP) under the four RCPs (RCP2.6, RCP4.5, RCP6.0, and RCP8.5). The Kappa statistic was used to evaluate model results by comparing simulations of baseline permafrost distribution (1981–2010) with the existing frozen soil maps. The comparison shows that the Surface Frost Number model is suitable for simulating the general characteristics of permafrost distribution on the QTP. Simulated results suggest that areas of permafrost degradation would be the smallest in the near-term (2011‒2040) with the rates of 17.17%, 18.07%, 12.95% and 15.66% under RCP2.6, RCP4.5, RCP6.0 and RCP8.5, respectively. The rate of permafrost degradation would be faster in the mid-term (2041‒2070), especially under the RCP8.5 scenario (about 41.42%). Areas of permafrost degradation would be the largest in the long-term (2071‒2099) relative to baseline conditions, with a modelled 64.31% decrease in permafrost distribution using the RCP8.5 scenario. Our results would help the decision‒making for engineering construction program on the QTP, and support local units in their efforts to adapt climate change.
Basic surface features of nuclear FKBPs facilitate chromatin binding
Authors: Leung Andrew , Jardim Francy-Pesek , Savic Neda , Monneau Yoan R. , González-Romero Rodrigo , Gudavicius Geoff , Eirin-Lopez Jose M. , Bartke Till , Mackereth Cameron D. , Ausió Juan , Nelson Christopher J. 
Published: June 2017

AbstractThe nucleoplasmin family of histone chaperones is identified by a pentamer-forming domain and multiple acidic tracts that mediate histone binding and chaperone activity. Within this family, a novel domain organization was recently discovered that consists of an N-terminal nucleoplasmin-like (NPL) domain and a C-terminal FKBP peptidyl-proline isomerase domain. Saccharomyces cerevisiae Fpr4 is one such protein. Here we report that in addition to its known histone prolyl isomerase activities, the Fpr4 FKBP domain binds to nucleosomes and nucleosome arrays in vitro. This ability is mediated by a collection of basic patches that enable the enzyme to stably associate with linker DNA. The interaction of the Fpr4 FKBP with recombinant chromatin complexes condenses nucleosome arrays independently of its catalytic activity. Based on phylogenetic comparisons we propose that the chromatin binding ability of ‘basic’ FKBPs is shared amongst related orthologues present in fungi, plants, and insects. Thus, a subclass of FKBP prolyl isomerase enzymes is recruited to linker regions of chromatin.
IR sensitivity enhancement of CMOS Image Sensor with diffractive light trapping pixels
Authors: Yokogawa Sozo , Oshiyama Itaru , Ikeda Harumi , Ebiko Yoshiki , Hirano Tomoyuki , Saito Suguru , Oinoue Takashi , Hagimoto Yoshiya , Iwamoto Hayato 
Published: June 2017

AbstractWe report on the IR sensitivity enhancement of back-illuminated CMOS Image Sensor (BI-CIS) with 2-dimensional diffractive inverted pyramid array structure (IPA) on crystalline silicon (c-Si) and deep trench isolation (DTI). FDTD simulations of semi-infinite thick c-Si having 2D IPAs on its surface whose pitches over 400 nm shows more than 30% improvement of light absorption at λ = 850 nm and the maximum enhancement of 43% with the 540 nm pitch at the wavelength is confirmed. A prototype BI-CIS sample with pixel size of 1.2 μm square containing 400 nm pitch IPAs shows 80% sensitivity enhancement at λ = 850 nm compared to the reference sample with flat surface. This is due to diffraction with the IPA and total reflection at the pixel boundary. The NIR images taken by the demo camera equip with a C-mount lens show 75% sensitivity enhancement in the λ = 700–1200 nm wavelength range with negligible spatial resolution degradation. Light trapping CIS pixel technology promises to improve NIR sensitivity and appears to be applicable to many different image sensor applications including security camera, personal authentication, and range finding Time-of-Flight camera with IR illuminations.
Automated Assessment of Child Vocalization Development Using LENA
Authors: Richards Jeffrey A. , Xu Dongxin , Gilkerson Jill , Yapanel Umit , Gray Sharmistha , Paul Terrance 
Published: June 2017

PurposeTo produce a novel, efficient measure of children's expressive vocal development on the basis of automatic vocalization assessment (AVA), child vocalizations were automatically identified and extracted from audio recordings using Language Environment Analysis (LENA) System technology.MethodAssessment was based on full-day audio recordings collected in a child's unrestricted, natural language environment. AVA estimates were derived using automatic speech recognition modeling techniques to categorize and quantify the sounds in child vocalizations (e.g., protophones and phonemes). These were expressed as phone and biphone frequencies, reduced to principal components, and inputted to age-based multiple linear regression models to predict independently collected criterion-expressive language scores. From these models, we generated vocal development AVA estimates as age-standardized scores and development age estimates.ResultAVA estimates demonstrated strong statistical reliability and validity when compared with standard criterion expressive language assessments.ConclusionsAutomated analysis of child vocalizations extracted from full-day recordings in natural settings offers a novel and efficient means to assess children's expressive vocal development. More research remains to identify specific mechanisms of operation.
Variables Influencing the Thrombostat 4000: Recommended Standardization
Authors: Dietrich Gerald , Kretschmer Volker , Weber Dagmar , Haupt Winfried , Langen Betiina , Huss Bernd 
Published: June 2017

The in vitro bleeding test (IVBT) (Thrombostat 4000) was performed on blood samples from healthy blood donors using a “preliminary standard test.” Only one factor of the test procedure was changed each time.We found the following parameters to have a significant influence on the test results: time interval between sampling and testing, diameter of the filter's aperture, capillary diameter, aggregating agents and their concentration, temperature of the blood and cartridges, method of drying the capillaries, the aspiration pressure, and the hematocrit of the blood sample. According to our data, we propose a standard IVBT for routine application and modifications for special diagnostic use.
DDAVP'S Shortening of the Bleeding Time Seems Due to Plasma von Willebrand Factor
Authors: Beck Karl-Heinz , Bleckmann Ulrich , Mohr Patrick , Kretschmer Volke 
Published: June 2017

The pathomechanism of desmopressin (DDAVP) dependent shortening of the acetylsalicylic acid (ASA) prolonged bleeding time (BT) is still unclear. The mechanism of action is supposed to be related to the plasma levels of von Willebrand factor (vWf), but a platelet effect is also discussed. We tried to investigate whether this effect of DDAVP might be due to either plasma vWF or platelets or both. We measured the effect of DDAVP (0.4 μg/ml bw in 250 ml 0.9% NaCI) on the Thrombostat 4000 (in vitro bleeding test (IVBT)) after each of 16 volunteers ingested 100 mg of ASA twice. To measure the possible effect ofDDAVP on plasma and platelets separately, a mixture of 800 μl of platelet poor plasma (PPP) or 200 μl of isolated platelets after DDAVP, respectively, was prepared and the IVBT monitored. Two hundred μl of PPP or platelets, respectively, served as a control. In an additional experiment, vWF concentrate (12 Vlml) dissolved in 200 μl of PPP after ASA ingestion, as described above, was added to 800 μl of citrated whole blood from the same volunteers.The addition of PPP after DDAVP as well as the PPP or PRP after ASA enriched with vWf led to a significant shortening of the IVBT in comparison to the control, indicating that the effect of DDAVP on the prolonged BT seems to be an isolated effect of plasma vWf. By adding platelets we were not able to show a direct effect of DDAVP on platelets.
Manipulation der Wirbelsäule: Wie gut ist diese Therapieform
belegt?
Authors: Ernst Edzard 
Published: June 2017

RECHERCHE:Professor Edzard Ernst, der Autor dieses Artikels, von der Universität Exeter
hat sich kritisch zur „Wirksamkeit der Manipulation“ geäußert 4. Stutzig machten ihn: die deutliche Diskrepanz zwischen den publizierten
Behauptungen und der Evidenz sowie die fehlende Plausibilität sogenannter „Subluxationen“
6
7. Hat er etwas
gegen diese Behandlung? Ist er zu subjektiv? Manche Manualtherapeuten meinen, ja. Ein
Anreiz für ihn, alle publizierten Cochrane Reviews zum Thema zusammenzufassen – für die
evidenzbasierte Therapie.
Manipulation oder nicht – ein Erfahrungsbericht
Authors: Ayachi Myma 
Published: June 2017

EFFEKTIVITÄT UND EFFIZIENZsind erstrebenswerte Ziele im Gesundheitswesen. Klinische
Muster helfen uns, sie zu erreichen – können unseren Blickwinkel aber auch verengen.
Paralleles Denken und ein offenes Clinical Reasoning erfordern ein Innehalten und helfen,
im entscheidenden Moment die Tunnelperspektive zu verlassen.
Bewegungsverhalten des N. auriculotemporalis während physiologischer Bewegungen der Mandibula
und der HWS
Authors: Schröer Melanie , von Piekartz Harry , Starke Wiebke 
Published: June 2017

ZusammenfassungDer N. mandibularis und seine Äste müssen sich bei Bewegungen des Kiefers und der HWS an
die veränderten umliegenden Strukturen anpassen, um die Funktionsfähigkeit zu erhalten.
Ziel dieser Querschnittstudie war herauszufinden, ob sich die Neurodynamik des N.
auriculotemporalis mithilfe von Sonografie untersuchen lässt. Dazu wurde bei 21 Probanden
der N. auriculotemporalis im Querschnitt (transversal) und in longitudinaler Ansicht
während verschiedener kombinierter Kiefer- und zervikaler Bewegungen aufgezeichnet und
beurteilt.Die transversale Untersuchung ergab die signifikant größten Bewegungen bei hochzervikaler
Flexion der HWS und gleichzeitiger Laterotrusion der Mandibula zur Gegenseite. Die
longitudinale Untersuchung zeigte eine vermehrte Bewegung des Nervs bei Einstellung der
HWS in hochzervikaler Flexion im Vergleich zur habituellen Position bei Depression und
Laterotrusion der Mandibula zur Gegenseite (nicht signifikant). Eine Korrelation zwischen
den Resultaten der transversalen longitudinalen Messung war nicht nachweisbar. Die
Ergebnisse lassen annehmen, dass vor allem hochzervikale Flexion, Laterotrusion und
Depression einen Einfluss auf die Dynamik des N. auriculotemporalis haben.
Einführung - Diagnostik und operative Therapie von
Gelenkknorpelschäden
Authors: Steinwachs Matthias , Guhlke-Steinwachs Ute , Mumme Marcus 
Published: June 2017

Zusammenfassung
Return to Sport Nach der Diagnose Knorpelschaden im Kniegelenk fürchten viele
Sportler, ihren Sport nicht mehr oder nur noch reduziert ausüben zu können. Wie eine
exakte Diagnostik, neue OP-Techniken und der richtige Zeitpunkt für Abhilfe sorgen, weiß
Matthias Steinwachs, der Züricher FIFA-Experte für Knorpelverletzungen und
-regeneration.