bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic profile of differentiated thyroid cancer in male and female patients with thyroidectomy during 2000–2013 in China: a retrospective study
Authors: Yan Hui-xian , Pang Ping , Wang Fu-lin , Tian Wen , Luo Yu-kun , Huang Wei , Yang Guo-qing , Jin Nan , Zang Li , Du Jin , Ba Jian-ming , Dou Jing-tao , Mu Yi-ming , Lyu Zhao-hui 
Published: November 2017

AbstractThe study aimed to investigate the gender-related differences of disease onset, age distribution, blood type, clinical characteristics, and malignant behaviors of differentiated thyroid carcinoma (DTC) in Chinese patients. A total of 7385 consecutive thyroid cancer patients who underwent thyroidectomy were retrospectively reviewed. 4087 (55.3%) were diagnosed as benign and the other (3298, 44.7%) were as malignant. DTC accounted for 97.6% in the malignant tumor. More single nodules turned out to be DTC in male compared to multiple nodules (46.9% vs. 40.4%, P = 0.004). The proportion increased along with the increase of year during 2000–2013, which was from 7.5% to 68.1% in males and from 16.2% to 66.7% in females. The level of preoperative TSH was significantly higher in patients with DTC compared to the patients with benign (1.97 vs. 1.57 mIU/L, P < 0.001). The proportion of thyroid cancer was dominated in blood type B and the lowest incidence in blood type A in male, the difference was not statistically significant. The results showed that age, nodule number, BMI and serum TSH were the related factors for DTC. More aggressive behaviors of DTC were observed in male patients, and more attention should be focused on the timely diagnosis and treatment of these patients.
Reversal of drug-resistance by noscapine chemo-sensitization in docetaxel resistant triple negative breast cancer
Authors: Doddapaneni Ravi , Patel Ketan , Chowdhury Nusrat , Singh Mandip 
Published: November 2017

AbstractMultidrug resistance (MDR) is a major impediment to cancer treatment. Here, for the first time, we investigated the chemo-sensitizing effect of Noscapine (Nos) at low concentrations in conjunction with docetaxel (DTX) to overcome drug resistance of triple negative breast cancer (TNBC). In vitro experiments showed that Nos significantly inhibited proliferation of TNBC wild type (p < 0.01) and drug resistant (p < 0.05) TNBC cells. Nos followed by DTX treatment notably increased the cell viability (~1.3 fold) markedly (p < 0.05) in 3D models compared to conventional 2D systems. In vivo oral administration of Nos (100 mg/kg) followed by intravenous DTX (5 mg/kg) liposome treatment revealed regression of xenograft tumors in both wild type (p < 0.001) and drug-resistant (p < 0.05) xenografts. In wild type xenografts, combination of Nos plus DTX group showed 5.49 and 3.25 fold reduction in tumor volume compared to Nos and DTX alone groups, respectively. In drug-resistant xenografts, tumor volume was decreased 2.33 and 1.41 fold in xenografts treated with Nos plus DTX significantly (p < 0.05) compared to Nos and DTX alone respectively and downregulated the expression of anti-apoptotic factors and multidrug resistance proteins. Collectively, chemo-sensitizing effect of Nos followed by DTX regime provide a promising chemotherapeutic strategy and its significant role for the treatment of drug-resistant TNBC.
MiR-17-5p and miR-20a promote chicken cell proliferation at least in part by upregulation of c-Myc via MAP3K2 targeting
Authors: Zhang Xiaofei , Song He , Qiao Shupei , Liu Jing , Xing Tianyu , Yan Xiaohong , Li Hui , Wang Ning 
Published: November 2017

AbstractThe miR-17-92 cluster has been well studied in mammals but less extensively studied in birds. Here, we demonstrated that miR-17-92 cluster overexpression promoted the proliferation of DF1 cells and immortalized chicken preadipocytes (ICPA-1), and miR-17-5p and miR-20a, members of the miR-17-92 cluster, targeted MAP3K2. Further analysis showed that MAP3K2 overexpression reduced the proliferation of DF1 and ICPA-1 cells and attenuated the promotive effect of the miR-17-92 cluster on cell proliferation. Downstream gene expression analysis of the MAPK signalling pathway showed that MAP3K2 overexpression decreased c-Myc expression; in contrast, MAP3K2 knockdown using RNA interference and miR-17-92 cluster overexpression increased c-Myc expression. Furthermore, c-Myc overexpression promoted miR-17-92 cluster expression and DF1 cell proliferation. Taken together, these data indicated that miR-17-92 promotes chicken cell proliferation at least in part by the upregulation of c-Myc via targeting MAP3K2, and the miR-17-92 cluster, c-Myc and E2F1 form a complex regulatory network in chicken cell proliferation.
Changes to Yucatán Peninsula precipitation associated with salinity and temperature extremes of the Caribbean Sea during the Maya civilization collapse
Authors: Wu Henry C. , Felis Thomas , Scholz Denis , Giry Cyril , Kölling Martin , Jochum Klaus P. , Scheffers Sander R. 
Published: November 2017

AbstractExplanations of the Classic Maya civilization demise on the Yucatán Peninsula during the Terminal Classic Period (TCP; ~CE 750–1050) are controversial. Multiyear droughts are one likely cause, but the role of the Caribbean Sea, the dominant moisture source for Mesoamerica, remains largely unknown. Here we present bimonthly-resolved snapshots of reconstructed sea surface temperature (SST) and salinity (SSS) variability in the southern Caribbean from precisely dated fossil corals. The results indicate pronounced interannual to decadal SST and SSS variability during the TCP, which may be temporally coherent to precipitation anomalies on the Yucatán. Our results are best explained by changed Caribbean SST gradients affecting the Caribbean low-level atmospheric jet with consequences for Mesoamerican precipitation, which are possibly linked to changes in Atlantic Meridional Overturning Circulation strength. Our findings provide a new perspective on the anomalous hydrological changes during the TCP that complement the oft-suggested southward displacement of the Intertropical Convergence Zone. We advocate for a strong role of Caribbean SST and SSS condition changes and related ocean-atmosphere interactions that notably influenced the propagation and transport of precipitation to the Yucatán Peninsula during the TCP.
Fine-mapping of genetic loci driving spontaneous clearance of hepatitis C virus infection
Authors: Huang Hailiang , Duggal Priya , Thio Chloe L. , Latanich Rachel , Goedert James J. , Mangia Alessandra , Cox Andrea L. , Kirk Gregory D. , Mehta Shruti , Aneja Jasneet , Alric Laurent , Donfield Sharyne M. , Cramp Matthew E. , Khakoo Salim I. , Tobler Leslie H. , Busch Michael , Alexander Graeme J. , Rosen Hugo R. , Edlin Brian R. , Segal Florencia P. , Lauer Georg M. , Thomas David L. , Daly Mark J. , Chung Raymond T. , Kim Arthur Y. 
Published: November 2017

AbstractApproximately three quarters of acute hepatitis C (HCV) infections evolve to a chronic state, while one quarter are spontaneously cleared. Genetic predispositions strongly contribute to the development of chronicity. We have conducted a genome-wide association study to identify genomic variants underlying HCV spontaneous clearance using ImmunoChip in European and African ancestries. We confirmed two previously reported significant associations, in the IL28B/IFNL4 and the major histocompatibility complex (MHC) regions, with spontaneous clearance in the European population. We further fine-mapped the association in the MHC to a region of about 50 kilo base pairs, down from 1 mega base pairs in the previous study. Additional analyses suggested that the association in MHC is stronger in samples from North America than those from Europe.
Graphene Quantum Dots for Cell Proliferation, Nucleus Imaging, and Photoluminescent Sensing Applications
Authors: Kumawat Mukesh Kumar , Thakur Mukeshchand , Gurung Raju B. , Srivastava Rohit 
Published: November 2017

AbstractWe report a simple one-pot microwave assisted “green synthesis” of Graphene Quantum Dots (GQDs) using grape seed extract as a green therapeutic carbon source. These GQDs readily self-assemble, hereafter referred to as “self-assembled” GQDs (sGQDs) in the aqueous medium. The sGQDs enter via caveolae and clathrin-mediated endocytosis and target themselves into cell nucleus within 6–8 h without additional assistance of external capping/targeting agent. The tendency to self-localize themselves into cell nucleus also remains consistent in different cell lines such as L929, HT-1080, MIA PaCa-2, HeLa, and MG-63 cells, thereby serving as a nucleus labelling agent. Furthermore, the sGQDs are highly biocompatible and act as an enhancer in cell proliferation in mouse fibroblasts as confirmed by in vitro wound scratch assay and cell cycle analysis. Also, photoluminescence property of sGQDs (lifetime circa (ca.) 10 ns) was used for optical pH sensing application. The sGQDs show linear, cyclic and reversible trend in its fluorescence intensity between pH 3 and pH 10 (response time: ~1 min, sensitivity −49.96 ± 3.5 mV/pH) thereby serving as a good pH sensing agent. A simple, cost-effective, scalable and green synthetic approach based sGQDs can be used to develop selective organelle labelling, nucleus targeting in theranostics, and optical sensing probes.
Carbon spheres@MnO2 core-shell nanocomposites with enhanced dielectric properties for electromagnetic shielding
Authors: Wang Huiya , Zhang Zhifan , Dong Chengjun , Chen Gang , Wang Yude , Guan Hongtao 
Published: November 2017

AbstractCarbon spheres (CS)@MnO2 core-shell nanocomposites, with MnO2 nanoflakes uniformly coating at the surface of CS cores, were successfully synthesized by a facile water-bathing method. MnO2 amounts is estimated to be 24.7 wt% in CS@MnO2 nanocomposites. A high dielectric loss value and an electromagnetic shielding effectiveness of 16‒23 dB were observed for the CS@MnO2 in the frequency range of 8‒18 GHz, which is mainly attributed to the enhanced absorption loss. The incorporation of the CS with MnO2 improves the electrical conductivity. Meanwhile, the electromagnetic impendence matching has been significantly ameliorated. Moreover, the increasing interfaces between the CS and MnO2 facilitate the microwave attenuation as well. Thus, the electromagnetic shielding performances were greatly enhanced. Our findings provide an effective methodology for the synthesis of the CS@MnO2 core-shell nanocomposite for potential electromagnetic applications.
A new paradigm in modelling the evolution of a stand via the distribution of tree sizes
Authors: Rupšys Petras , Petrauskas Edmundas 
Published: November 2017

AbstractOur study focusses on investigating a modern modelling paradigm, a bivariate stochastic process, that allows us to link individual tree variables with growth and yield stand attributes. In this paper, our aim is to introduce the mathematics of mixed effect parameters in a bivariate stochastic differential equation and to describe how such a model can be used to aid our understanding of the bivariate height and diameter distribution in a stand using a large dataset provided by the Lithuanian National Forest Inventory (LNFI). We examine tree height and diameter evolution with a Vasicek-type bivariate stochastic differential equation and mixed effect parameters. It is focused on demonstrating how new developed bivariate conditional probability density functions allowed us to calculate the evolution, in the forward and backward directions, of the mean diameter, height, dominant height, assortments, stem volume of a stand and uncertainties in these attributes for a given stand age. We estimate the parameters by considering discrete samples of the diameter and height at a given age and by using an approximated maximum likelihood procedure. The model performance criteria for the height and diameter growth models include statistical indexes and an analysis of residuals.
Validation of methods for prediction of clinical output levels of active middle ear implants from measurements in human cadaveric ears
Authors: Grossöhmichen Martin , Waldmann Bernd , Salcher Rolf , Prenzler Nils , Lenarz Thomas , Maier Hannes 
Published: November 2017

AbstractToday, the standard method to predict output levels of active middle ear implants (AMEIs) before clinical data are available is stapes vibration measurement in human cadaveric ears, according to ASTM standard F2504-05. Although this procedure is well established, the validity of the predicted output levels has never been demonstrated clinically. Furthermore, this procedure requires a mobile and visually accessible stapes and an AMEI stimulating the ossicular chain. Thus, an alternative method is needed to quantify the output level of AMEIs in all other stimulation modes, e.g. reverse stimulation of the round window. Intracochlear pressure difference (ICPD) is a good candidate for such a method as it correlates with evoked potentials in animals and it is measurable in cadaveric ears. To validate this method we correlated AMEI output levels calculated from ICPD and from stapes vibration in cadaveric ears with outputs levels determined from clinical data. Output levels calculated from ICPD were similar to output levels calculated from stapes vibration and almost identical to clinical data. Our results demonstrate that both ICPD and stapes vibration can be used as a measure to predict AMEI clinical output levels in cadaveric ears and that ICPD as reference provided even more accurate results.
Keratoconus Detection Based on a New Corneal Volumetric Analysis
Authors: Cavas-Martínez Francisco , Bataille Laurent , Fernández-Pacheco Daniel G. , Cañavate Francisco J. F. , Alio Jorge L. 
Published: November 2017

AbstractThere are numerous tomographic indices for the detection of keratoconus risk. When the indexes based on corneal volume are analyzed, two problems are presented: on the one hand, they are not very sensitive to the detection of incipient cases of keratoconus because they are not locally defined in the primary developmental region of the structural abnormalities; and on the other hand, they do not register the geometric decompensation driven by the asymmetry present during the disease progression. This work performed a morphogeometric modeling of the cornea by the aid of CAD tools and using raw topographic data (Sirius system, CSO, Firenze). For this method, four singular points present on the corneal surfaces were located and the following parameters based on corneal volume were calculated: VOLmct, defined by the points of minimal thickness; VOLaap, defined by the anterior corneal apex, and VOLpap, defined by the posterior corneal apex. The results demonstrate that a further reduction of corneal volume in keratoconus happens and significantly progresses along the disease severity level. The combination of optical and volumetric data, that collect the sensitivity of the asymmetry generated by the disease, allows an accurate detection of incipient cases and follow up of the disease progression.
PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens
Authors: Spahn Philipp N. , Bath Tyler , Weiss Ryan J. , Kim Jihoon , Esko Jeffrey D. , Lewis Nathan E. , Harismendy Olivier 
Published: November 2017

AbstractLarge-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy analysis option. While a few bioinformatic tools have been developed for this purpose, their utility is still hindered either due to limited functionality or the requirement of bioinformatic expertise. To make sequencing data analysis of CRISPR/Cas9 screens more accessible to a wide range of scientists, we developed a Platform-independent Analysis of Pooled Screens using Python (PinAPL-Py), which is operated as an intuitive web-service. PinAPL-Py implements state-of-the-art tools and statistical models, assembled in a comprehensive workflow covering sequence quality control, automated sgRNA sequence extraction, alignment, sgRNA enrichment/depletion analysis and gene ranking. The workflow is set up to use a variety of popular sgRNA libraries as well as custom libraries that can be easily uploaded. Various analysis options are offered, suitable to analyze a large variety of CRISPR/Cas9 screening experiments. Analysis output includes ranked lists of sgRNAs and genes, and publication-ready plots. PinAPL-Py helps to advance genome-wide screening efforts by combining comprehensive functionality with user-friendly implementation. PinAPL-Py is freely accessible at http://pinapl-py.ucsd.edu with instructions and test datasets.
Arc-induced Long Period Gratings in standard and speciality optical fibers under mixed neutron-gamma irradiation
Authors: Stancălie Andrei , Esposito Flavio , Ranjan Rajeev , Bleotu Petrişor , Campopiano Stefania , Iadicicco Agostino , Sporea Dan 
Published: November 2017

AbstractIn this paper, for the first time, the effects of mixed neutron and gamma flux on the spectral and sensing responses of Long Period Gratings (LPGs) are thoroughly analyzed. Six LPGs written by means of Electric Arc Discharge (EAD) technique in standard and speciality fibers, including radiation-hardened ones, were tested. The EAD technique was chosen because it enables the writing of gratings both in standard and not photosensitive fibers. The experiments have been carried out in a “TRIGA” pulsed nuclear reactor and the LPGs were irradiated by a gamma-ray dose-rate of 9 Gy/s and a mean 1.2∙1012 n/(cm2s) neutron flux. Real time monitoring was performed for a comparative investigation of LPGs’ response, in terms of radiation sensitivity and wavelength shift. Experiments show that LPG in a radiation-resistant fiber exhibits resonant wavelength shift higher than LPG in standard fiber. The changes of temperature sensitivity due to radiation were experimentally established by comparison of pre- and post-radiation characterization, indicating that radiation effects induce a slight increase of the temperature sensitivity, except for the LPG in pure-silica fiber. Theoretical and numerical analysis was combined with experimental data for evaluation LPGs’ parameters changes, such as refractive index and thermo-optic coefficient, after exposure to radiation.
Computational study on single molecular spectroscopy of tyrosin-glycine, tryptophane-glycine and glycine-tryptophane
Authors: Yang Bing , Liu Shixue , Lin Zijing 
Published: November 2017

AbstractQuantum chemistry calculations play a fundamental role in revealing the molecular structures observed in gas-phase spectroscopic measurements. The supersonic jet cooling widely used in single molecular spectroscopy experiment is a non-equilibrium process and often causes confusion on the theoretical and experimental comparison. A computational approach is proposed here to account for the effect of the non-equilibrium cooling on the experimental spectra and applied to the cases of tyrosin-glycine (YG), tryptophane-glycine (WG) and glycine-tryptophane (GW). The low energy conformers of YG, WG and GW are obtained through thorough conformational searches. The structural features and equilibrium distributions of conformations and the energy barriers for conformer conversions are then determined. Three classes of transition energy barriers, high, medium and low, are found for the conversions among conformers with distinctly different, similar and the same structural types, respectively. The final conformation populations are determined by assuming an initial temperature of about 450 K and allowing for only the conformation conversion with a low energy barrier to occur during the rapid cooling process. The results provide a natural explanation for the numbers of YG, WG and GW conformations observed experimentally. The theoretical conformation assignments are also in good agreement with the experimental IR data.
Facial Aesthetics in Young Adults after Cleft Lip and Palate Treatment over Five Decades
Authors: Sinko K. , Cede J. , Jagsch R. , Strohmayr A. L. , McKay A. , Mosgoeller W. , Klug C. 
Published: November 2017

AbstractCleft Lip and Palate (CLP) - a common facial malformation in newborns – is typically corrected by surgical intervention to allow for normal speech development, psychosocial adjustment, and facial attractiveness. The long term treatment outcome can be evaluated after a number of years, possibly in adulthood. We investigated the aesthetics of the nasolabial region by subjective ratings. To compare various surgical approaches we recruited 12 raters to evaluate 429 patients. Expert and lay raters judged photographs from patients, who have completed treatment with one of three different surgical strategies performed in our institution over 50 years. Facial photographs were cropped, presented to the raters in a randomized sequence, and judged by the raters on a 5 point Likert scale. The subjective ratings between the raters revealed a fair to substantial inter-rater reliability. The average ratings of the surgical outcome improved continuously over the investigated 5 decades. Despite possible differences between raters and rater groups this overall result was consistently seen in the gender groups (male/female), or expertise related groups (expert/lay). Our analysis revealed that patients with bilateral CLP scored worse than patients with unilateral CLP when treated in the fifties; more recently treated patients of both groups scored similarly.
Early meteorological records from Latin-America and the Caribbean during the 18th and 19th centuries
Authors: Domínguez-Castro Fernando , Vaquero José Manuel , Gallego María Cruz , Farrona Ana María Marín , Antuña-Marrero Juan Carlos , Cevallos Erika Elizabeth , Herrera Ricardo García , de la Guía Cristina , Mejía Raúl David , Naranjo José Manuel , del Rosario Prieto María , Ramos Guadalupe Luis Enrique , Seiner Lizardo , Trigo Ricardo Machado , Villacís Marcos 
Published: November 2017

AbstractThis paper provides early instrumental data recovered for 20 countries of Latin-America and the Caribbean (Argentina, Bahamas, Belize, Brazil, British Guiana, Chile, Colombia, Costa Rica, Cuba, Ecuador, France (Martinique and Guadalupe), Guatemala, Jamaica, Mexico, Nicaragua, Panama, Peru, Puerto Rico, El Salvador and Suriname) during the 18th and 19th centuries. The main meteorological variables retrieved were air temperature, atmospheric pressure, and precipitation, but other variables, such as humidity, wind direction, and state of the sky were retrieved when possible. In total, more than 300,000 early instrumental data were rescued (96% with daily resolution). Especial effort was made to document all the available metadata in order to allow further post-processing. The compilation is far from being exhaustive, but the dataset will contribute to a better understanding of climate variability in the region, and to enlarging the period of overlap between instrumental data and natural/documentary proxies.