bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence and predictors of in-stent restenosis following intervention for pulmonary vein stenosis due to fibrosing mediastinitis
Authors: Jia Mengfei , Su Hongling , Jiang Kaiyu , Wang Aqian , Guo Zhaoxia , Zhu Hai , Zhang Fu , Sun Xuechun , Shi Yiwei , Pan Xin , Cao Yunshan 
Published: October 2024

Abstract
Background
Fibrosing mediastinitis (FM) is a rare yet fatal condition, caused by different triggers and frequently culminating in the obstruction of the pulmonary vasculature and airways, often leading to pulmonary hypertension and right heart failure. Percutaneous transluminal pulmonary venoplasty (PTPV) is an emerging treatment for pulmonary vein stenosis (PVS) caused by FM. Our previous study showed as high as 24% of in-stent restenosis (ISR) in FM. However, the predictors of ISR are elusive.

Objectives
We sought to identify the predictors of ISR in patients with PVS caused by extraluminal compression due to FM.

Methods
We retrospectively enrolled patients with PVS-FM who underwent PTPV between July 1, 2018, and December 31, 2022. According to ISR status, patients were divided into two groups: the ISR group and the non-ISR group. Baseline characteristics (demographics and lesions) and procedure-related information were abstracted from patient records and analyzed. Univariate and multivariate analyses were performed to determine the predictors of ISR.

Results
A total of 142 stents were implanted in 134 PVs of 65 patients with PVS-FM. Over a median follow-up of 6.6 (3.4–15.7) months, 61 of 134 PVs suffered from ISR. Multivariate analysis demonstrated a significantly lower risk of ISR in PVs with a larger reference vessel diameter (RVD) (odds ratio (OR): 0.79; 95% confidence interval [CI]: 0.64 to 0.98; P = 0.032), and stenosis of the corresponding pulmonary artery (Cor-PA) independently increased the risk of restenosis (OR: 3.41; 95% CI: 1.31 to 8.86; P = 0.012). The cumulative ISR was 6.3%, 21.4%, and 39.2% at the 3-, 6-, and 12-month follow-up, respectively.

Conclusion
ISR is very high in PVS-FM, which is independently associated with RVD and Cor-PA stenosis.

Trail Registration
Chinese Clinical Trials Register; No.: ChiCTR2000033153. URL: http://www.chictr.org.cn.

Graphical Abstract
Promising predictors of diabetic peripheral neuropathy in children and adolescents with type 1 diabetes mellitus
Authors: Abo Hola Ahmed S. , Abd El Naby Sameh A. , Allam Esraa T. , Gab Allah Ayaat A. , Hammad Dina A. 
Published: October 2024

Abstract
Background
Diabetic peripheral neuropathy (DPN) in children and adolescents with type 1 diabetes mellitus (T1DM) is a growing issue, with controversial data in the terms of prevalence and evaluation timelines. Currently, there are no clear standards for its early detection. Therefore, our aim was to assess the contribution of the Michigan neuropathy screening instrument (MNSI), lipid profile, serum neuron specific enolase (NSE), and serum heat shock protein 27 (HSP 27) to the prediction of DPN in children and adolescents with T1DM.

Methods
In this case-control study, fifty children diagnosed with T1DM for at least five years were enrolled and evaluated through complete neurological examination, MNSI, and nerve conduction study (NCS). Additionally, HbA1c, lipid profile, serum NSE, and serum HSP 27 levels were measured for patients and controls.

Results
The prevalence of DPN in our study was 24% by NCS, and electrophysiological changes showed a statistically significant lower conduction velocity for the posterior tibial and sural nerves, as well as a prolonged latency period for the common peroneal and sural nerves in neuropathic patients. In these patients, older age, earlier age of diabetes onset, longer disease duration, higher total cholesterol, triglycerides, low density lipoprotein cholesterol, HbA1c, serum NSE, and HSP27 levels were observed. The MNSI examination score ≥ 1.5 cutoff point had an area under the curve (AUC) of 0.955, with 75% sensitivity and 94.74% specificity, according to receiver operating characteristic curve analysis. However, the questionnaire’s cutoff point of ≥ 5 had an AUC of 0.720, 75% sensitivity, and 63% specificity, with improved overall instrument performance when combining both scores. Regarding blood biomarkers, serum NSE had greater sensitivity and specificity in discriminating neuropathic patients than HSP27 (92% and 74% versus 75% and 71%, respectively). Regression analysis revealed a substantial dependency for MNSI and serum NSE in predicting DPN in patients.

Conclusions
Despite limited research in pediatrics, MNSI and serum NSE are promising predictive tools for DPN in children and adolescents with T1DM, even when they are asymptomatic. Poor glycemic control and lipid profile changes may play a critical role in the development of DPN in these patients, despite conflicting results in various studies.
Elevated Aβ aggregates in feces from Alzheimer’s disease patients: a proof-of-concept study
Authors: Pils Marlene , Dybala Alexandra , Schaffrath Anja , Rehn Fabian , Kutzsche Janine , Blömeke Lara , Tusche Markus , Özdüzenciler Pelin , Bujnicki Tuyen , Kraemer-Schulien Victoria , Gramespacher Hannes , Schmieschek Maximilian H.T. , Barbe Michael T. , Onur Oezguer A. , Fink Gereon R. , Tamgüney Gültekin , Bannach Oliver , Willbold Dieter 
Published: October 2024

Abstract
Background
Misfolding and aggregation of amyloid β (Aβ), along with neurofibrillary tangles consisting of aggregated Tau species, are pathological hallmarks of Alzheimer’s disease (AD) onset and progression. In this study, we hypothesized the clearance of Aβ aggregates from the brain and body into the gut.

Methods
To investigate this, we used surface-based fluorescence intensity distribution analysis (sFIDA) to determine the Aβ aggregate concentrations in feces from 26 AD patients and 31 healthy controls (HC).

Results
Aβ aggregates were detectable in human feces and their concentrations were elevated in AD patients compared to HC (specificity 90.3%, sensitivity 53.8%).

Conclusion
Thus, fecal Aβ aggregates constitute a non-invasive biomarker candidate for diagnosing AD. Whether digestion-resistant Aβ aggregates in feces are secreted via the liver and bile or directly from the enteric neuronal system remains to be elucidated.
Extracellular vesicles as therapeutic tools in regenerative dentistry
Authors: Xia Evelyn Jingwen , Zou Shasha , Zhao Xiu , Liu Wei , Zhang Yang , Zhao Irene Shuping 
Published: October 2024

AbstractDental and maxillofacial diseases are always accompanied by complicated hard and soft tissue defects, involving bone, teeth, blood vessels and nerves, which are difficult to repair and severely affect the life quality of patients. Recently, extracellular vesicles (EVs) secreted by all types of cells and extracted from body fluids have gained more attention as potential solutions for tissue regeneration due to their special physiological characteristics and intrinsic signaling molecules. Compared to stem cells, EVs present lower immunogenicity and tumorigenicity, cause fewer ethical problems, and have higher stability. Thus, EV therapy may have a broad clinical application in regenerative dentistry. Herein, we reviewed the currently available literature regarding the functional roles of EVs in oral and maxillofacial tissue regeneration, including in maxilla and mandible bone, periodontal tissues, temporomandibular joint cartilage, dental hard tissues, peripheral nerves and soft tissues. We also summarized the underlying mechanisms of actions of EVs and their delivery strategies for dental tissue regeneration. This review would provide helpful guidelines and valuable insights into the emerging potential of EVs in future research and clinical applications in regenerative dentistry.
Exercise, disease state and sex influence the beneficial effects of Fn14-depletion on survival and muscle pathology in the SOD1G93A amyotrophic lateral sclerosis (ALS) mouse model
Authors: Hazell Gareth , McCallion Eve , Ahlskog Nina , Sutton Emma R. , Okoh Magnus , Shaqoura Emad I. H. , Hoolachan Joseph M. , Scaife Taylor , Iqbal Sara , Bhomra Amarjit , Kordala Anna J. , Scamps Frederique , Raoul Cedric , Wood Matthew J. A. , Bowerman Melissa 
Published: October 2024

Abstract
Background
Amyotrophic lateral sclerosis (ALS) is a devastating and incurable neurodegenerative disease. Accumulating evidence strongly suggests that intrinsic muscle defects exist and contribute to disease progression, including imbalances in whole-body metabolic homeostasis. We have previously reported that tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) and fibroblast growth factor inducible 14 (Fn14) are significantly upregulated in skeletal muscle of the SOD1G93A ALS mouse model. While antagonising TWEAK did not impact survival, we did observe positive effects in skeletal muscle. Given that Fn14 has been proposed as the main effector of the TWEAK/Fn14 activity and that Fn14 can act independently from TWEAK in muscle, we suggest that manipulating Fn14 instead of TWEAK in the SOD1G93A ALS mice could lead to differential and potentially improved benefits.

Methods
We thus investigated the contribution of Fn14 to disease phenotypes in the SOD1G93A ALS mice. To do so, Fn14 knockout mice (Fn14−/−) were crossed onto the SOD1G93A background to generate SOD1G93A;Fn14−/− mice. Investigations were performed on both unexercised and exercised (rotarod and/or grid test) animals (wild type (WT), Fn14−/−, SOD1G93A and SOD1G93A;Fn14−/−).

Results
Here, we firstly confirm that the TWEAK/Fn14 pathway is dysregulated in skeletal muscle of SOD1G93A mice. We then show that Fn14-depleted SOD1G93A mice display increased lifespan, myofiber size, neuromuscular junction endplate area as well as altered expression of known molecular effectors of the TWEAK/Fn14 pathway, without an impact on motor function. Importantly, we also observe a complex interaction between exercise (rotarod and grid test), genotype, disease state and sex that influences the overall effects of Fn14 deletion on survival, expression of known molecular effectors of the TWEAK/Fn14 pathway, expression of myosin heavy chain isoforms and myofiber size.

Conclusions
Our study provides further insights on the different roles of the TWEAK/Fn14 pathway in pathological skeletal muscle and how they can be influenced by age, disease, sex and exercise. This is particularly relevant in the ALS field, where combinatorial therapies that include exercise regimens are currently being explored. As such, a better understanding and consideration of the interactions between treatments, muscle metabolism, sex and exercise will be of importance in future studies.
Matched-pair analysis of mCRPC patients receiving 177Lu-labeled PSMA-targeted radioligand therapy in a 4-week versus 6-week treatment interval
Authors: Karimzadeh Amir , Hecker Charlotte-Sophie , Heck Matthias M. , Tauber Robert , D’Alessandria Calogero , Weber Wolfgang A. , Eiber Matthias , Rauscher Isabel 
Published: October 2024

Abstract
Background
The optimal regimen for 177Lu-labeled prostate-specific membrane antigen-targeted radioligand therapy, including treatment intervals, remains under study, with evidence suggesting shorter intervals could benefit patients with high disease volume and rapid progression. This retrospective analysis evaluated treatment toxicity, PSA response, PSA-progression-free survival (PSA-PFS), and overall survival (OS) in matched cohorts of mCRPC patients receiving 177Lu-PSMA-RLT at 4-week versus 6-week intervals.

Results
A PSA response (PSA decline ≥ 50%) was achieved in 47.8% and 21.7% of patients in the 4-week and 6-week treatment interval groups, respectively (p = 0.12). There was a trend towards longer PSA-PFS in the 4-week group compared to the 6-week group (median PSA-PFS, 26.0 weeks vs. 18.0 weeks; HR 0.6; p = 0.2). Although not statistically significant, there was a trend towards shorter OS in the 4-week group compared to the 6-week group (median OS, 15.1 months vs. 18.4 months; HR 1.3; p = 0.5). The 4-week group had a significantly greater decrease in leucocyte and platelet counts compared to the 6-week group (38.5% vs. 18.2% and 26.7% vs. 10.7%; p = 0.047 and p = 0.02). Severe adverse events were modest in both groups.

Conclusions
Intensifying treatment intervals from 6 weeks to 4 weeks showed some improvements in PSA response and PSA-PFS for mCRPC patients, but did not significantly affect OS. Additionally, bone marrow reserve was significantly reduced with the intensified regimen. Therefore, the overall benefit remains uncertain, and further prospective studies are needed to compare 4-week and 6-week intervals regarding toxicity, treatment response, and outcome.
Machine Learning Approach to Rapidly Evaluate Curling of Concrete Pavement
Authors: Han Sangyoung , Heo Taemin , Yeum Chul Min , Kim Kukjoo , Choi Jongkwon , Tia Mang 
Published: October 2024

AbstractThis paper focuses on the methodology for evaluating the degree of total curling in concrete pavement using machine learning. Deflection induced by falling weight deflectometer (FWD) testing is known as a direct correlation to total curling including built-in and daily curling. However, deflection measurement in the in-service road is also affected by others, such as environmental conditions, pavement geometry, subgrade stiffness, and mixture design. Thus, it is challenging to determine the level of curling from FWD data due to the complexity of influencing parameters. To navigate this complexity, prominent machine learning models are exploited to identify a non-linear relationship between curling and FWD deflections. A finite-element simulation of FWD is conducted to generate a vast data set, and a robust regression model is trained to estimate the total effective temperature difference (TETD) to quantify the effects of curling. Since input parameters for testing pavements can be measurable in the field, curling from TETD can be readily obtained using the proposed methodology. Comparative simulations highlight that the proposed models, with an MAE less than 0.5 °C significantly outperform the multiple regression performance, which registers an MAE of 3.45 °C in TETD, particularly in offering cost-effective and noise-tolerant prediction.
The detection, biological function, and liquid biopsy application of extracellular vesicle-associated DNA
Authors: Guo Shan , Wang Xin , Shan Danni , Xiao Yu , Ju Lingao , Zhang Yi , Wang Gang , Qian Kaiyu 
Published: October 2024

AbstractCell-derived extracellular vesicles (EVs), which carry diverse biomolecules such as nucleic acids, proteins, metabolites, and lipids reflecting their cell of origin, are released under both physiological and pathological conditions. EVs have been demonstrated to mediate cell-to-cell communication and serve as biomarkers. EV-associated DNA (EV-DNA) comprises genomic and mitochondrial DNA (i.e., gDNA and mtDNA) fragments. Some studies have revealed that EV-DNA can represent the full nuclear genome and mitochondrial genome of parental cells. Furthermore, DNA fragments loaded into EVs are stable and can be transferred to recipient cells to regulate their biological functions. In this review, we summarized and discussed EV-DNA research advances with an emphasis on EV-DNA detection at the population-EV and single-EV levels, gene transfer-associated biological functions, and clinical applications as biomarkers for disease liquid biopsy. We hope that this review will provide potential directions or guidance for future EV-DNA investigations.
Styled and characteristic Peking opera facial makeup synthesis with co-training and transfer conditional styleGAN2
Authors: Shen Yinghua , Duan Oran , Xin Xiaoyu , Yan Ming , Li Zhe 
Published: October 2024

Abstract
Against the backdrop of the deep integration of culture and technology, research and practice in digitization of intangible cultural heritage has continued to deepen. However, due to the lack of data and training, it is still very difficult to apply artificial intelligence to the field of cultural heritage protection. This article integrates image generation technology into the digital protection of Peking opera facial makeup, using a self-built Peking opera facial makeup dataset. Based on the StyleGAN2 network, we propose a style generative cooperative training network Co-StyleGAN2, which integrates the adaptive data augmentation (ADA) to alleviate the problem of discriminator overfitting and introduces the idea of cooperative training to stabilize the training process. We design a Peking opera facial makeup image transform conditional generation network TC-StyleGAN2 which is transferred from unconditional generation network. The weights of the unconditional pre-training model are fixed, and an adaptive filtering modulation module is added to modulate the category parameters to complete the conversion from unconditional to conditional StyleGAN2 to deal with the training difficulty of conditional GANs on limited data, which suffer from severe mode collapse. The experimental results show that the proposed training strategy is better than the comparison algorithms, and the image generation quality and diversity have been improved.
3D ultrasound guidance for radiofrequency ablation in an anthropomorphic thyroid nodule phantom
Authors: Boers Tim , Braak Sicco J. , Brink Wyger M. , Versluis Michel , Manohar Srirang 
Published: October 2024

Abstract
Background
The use of two-dimensional (2D) ultrasound for guiding radiofrequency ablation (RFA) of benign thyroid nodules presents limitations, including the inability to monitor the entire treatment volume and operator dependency in electrode positioning. We compared three-dimensional (3D)-guided RFA using a matrix ultrasound transducer with conventional 2D-ultrasound guidance in an anthropomorphic thyroid nodule phantom incorporated additionally with temperature-sensitive albumin.

Methods
Twenty-four phantoms with 48 nodules were constructed and ablated by an experienced radiologist using either 2D- or 3D-ultrasound guidance. Postablation T2-weighted magnetic resonance imaging scans were acquired to determine the final ablation temperature distribution in the phantoms. These were used to analyze ablation parameters, such as the nodule ablation percentage. Further, additional procedure parameters, such as dominant/non-dominant hand use, were recorded.

Results
Nonsignificant trends towards lower ablated volumes for both within (74.4 ± 9.1% (median ± interquartile range) versus 78.8 ± 11.8%) and outside of the nodule (0.35 ± 0.18 mL versus 0.45 ± 0.46 mL), along with lower variances in performance, were noted for the 3D-guided ablation. For the total ablation percentage, 2D-guided dominant hand ablation performed better than 2D-guided non-dominant hand ablation (81.0% versus 73.2%, p = 0.045), while there was no significant effect in the hand comparison for 3D-guided ablation.

Conclusion
3D-ultrasound-guided RFA showed no significantly different results compared to 2D guidance, while 3D ultrasound showed a reduced variance in RFA. A significant reduction in operator-ablating hand dependence was observed when using 3D guidance. Further research into the use of 3D ultrasound for RFA is warranted.

Relevance statement
Using 3D ultrasound for thyroid nodule RFA could improve the clinical outcome. A platform that creates 3D data could be used for thyroid diagnosis, therapy planning, and navigational tools.

Key Points

Twenty-four in-house-developed thyroid nodule phantoms with 48 nodules were constructed.

RFA was performed under 2D- or 3D-ultrasound guidance.

3D- and 2D ultrasound-guided RFAs showed comparable performance.

Real-time dual-plane imaging may offer an improved overview of the ablation zone and aid electrode positioning.

Dominant and non-dominant hand 3D-ultrasound-guided RFA outcomes were comparable.

Graphical Abstract
Apoptosis and genotoxicity effects of Zygophyllum album and Suaeda palaestina extracts on Ehrlich solid carcinoma in mice
Authors: El-Attar Marwa M. , Ahmad Ekram S. , Kamel Hedaya A. , Hassan Amal I. , Awad Abdel-Fattah A. , Abdel-Tawab Fatthy M. 
Published: October 2024

Abstract
Background
Various medicinal plants and their bioactive compounds exhibited promising anticancer activities by inducing apoptosis, inhibiting angiogenesis, and modulating several signaling pathways in cancer cells. This study aims to assess whether two medicinal plant extracts have anticancer properties, Suaeda Palaestina and Zygophyllum album.

Methods
This study used Ehrlich solid tumor mice as its in vivo model. We divided male mice into five groups (n = 5 per group). Group I was used as a control for Ehrlich ascites carcinoma (EAC). Groups 2 and 3 were given Z. album extract 180 mg/kg and 360 mg/kg body weight intraperitoneally. Groups 4 and 5 were given the same dose of S. palaestina and treated three times a week for 2 weeks, starting on day 10 after EAC implantation. After 3 weeks, we collected blood samples and thigh skeletal muscle, homogenized them, and processed them for analysis. The results showed that Ehrlich solid rats (EST) treated with low-dose dichloromethane extracts from Z. album and S. palaestina had significantly smaller tumor sizes than the control group. Protein expression levels of p53, caspase 3, and Bcl-2 were quantified by western blotting.

Results
The extracts from both plants induced the hunger mechanism, leading to increased expression of p53 and caspase 3 and decreased expression of Bcl-2 at the protein level in EST mice treated with Z. album and S. palaestina. In addition, the comet assay indicated that these plants have a genotoxic potential for solid tumor cells. The T3 and T4 levels in EST blood samples revealed that both plants had significantly reduced the concentration of T3 and significantly increased T4 compared to the EST mice untreated group. Furthermore, these results showed that Z. album and S. palaestina had antiproliferative effects in EST mice through apoptosis-mediated genotoxicity.

Conclusions
These findings indicated that S. palaestina and Z. album could be considered potential natural sources of anticancer agents.
Can individual functional improvements be predicted in osteoarthritic patients after total knee arthroplasty?
Authors: Kim Sung Eun , Ro Du Hyun , Lee Myung Chul , Han Hyuk-Soo 
Published: October 2024

Abstract
Purpose
Total knee arthroplasty (TKA) is an effective treatment for advanced osteoarthritis, and achieving optimal outcomes can be challenging due to various influencing factors. Previous research has focused on identifying factors that affect postoperative functional outcomes. However, there is a paucity of studies predicting individual postoperative improvement following TKA. Therefore, a quantitative prediction model for individual patient outcomes is necessary.

Materials and methods
Demographic data, radiologic variables, intraoperative variables, and physical examination findings were collected from 976 patients undergoing TKA. Preoperative and 1-year postoperative Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores were assessed, and multivariate regression analysis was conducted to identify significant factors influencing one-year WOMAC scores and changes in WOMAC scores. A predictive model was developed on the basis of the findings.

Results
The predictive accuracy of the model for 1-year WOMAC scores was poor (all adjusted R2 < 0.08), whereas the model for changes in WOMAC scores demonstrated strong predictability (all adjusted R2 > 0.75). Preoperative WOMAC scores, sex, and postoperative knee range of motion significantly affected all pain, stiffness, and physical function aspects of the WOMAC scores (all P < 0.05). Age, cerebrovascular disease, and patellar resurfacing were associated with changes in physical function (all P < 0.05).

Conclusions
The developed quantitative model demonstrated high accuracy in predicting changes in WOMAC scores after TKA. The identified factors influencing postoperative improvement in WOMAC scores can assist in optimizing patient outcomes after TKA.