bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Eco‐Friendly, Tunable and Scalable Method for Producing Porous Functional Nanomaterials Designed Using Molecular Interactions
Authors: Manning Joseph R. H. , Yip Thomas W. S. , Centi Alessia , Jorge Miguel , Patwardhan Siddharth V. 
Published: February 2017

AbstractDespite significant improvements in the synthesis of templated silica materials, post‐synthesis purification remains highly expensive and renders the materials industrially unviable. In this study this issue is addressed for porous bioinspired silica by developing a rapid room‐temperature solution method for complete extraction of organic additives. Using elemental analysis and N2 and CO2 adsorption, the ability to both purify and controllably tailor the composition, porosity and surface chemistry of bioinspired silica in a single step is demonstrated. For the first time the extraction is modelled using molecular dynamics, revealing that the removal mechanism is dominated by surface‐charge interactions. This is extended to other additive chemistry, leading to a wider applicability of the method to other materials. Finally the environmental benefits of the new method are estimated and compared with previous purification techniques, demonstrating significant improvements in sustainability.
Spatial planning for fisheries in the Northern Adriatic: working toward viable and sustainable fishing
Authors: Bastardie Francois , Angelini Silvia , Bolognini Luca , Fuga Federico , Manfredi Chiara , Martinelli Michela , Nielsen J. Rasmus , Santojanni Alberto , Scarcella Giuseppe , Grati Fabio 
Published: February 2017

AbstractGiven the great overfishing of the demersal resources in the Northern Adriatic Sea (geographical sub‐area [GSA] 17), along with the fishing pressure in marine habitats, evidence strongly supports the need to evaluate appropriate management approaches. Several fishing activities operate simultaneously in the area, and the need to minimize conflicts among them is also a social concern. We applied a spatially and temporally explicit fish and fisheries model to assess the impact of a suite of spatial plans suggested by practitioners that could reduce the pressure on the four demersal stocks of high commercial interest in the GSA 17 and that could promote space sharing between mutually exclusive activities. We found that excluding trawlers from some areas has lowered the effective fishing effort, resulting in some economic losses but providing benefit to the set netters. Not every simulated fishing vessel is impacted in the same way because some fishing communities experienced different economic opportunities, particularly when a 6‐nautical mile buffer zone from the coast was implemented in the vicinity of important fishing grounds. Along this buffer zone, the four stocks were only slightly benefiting from the protection of the area and from fewer discards. In contrast, assuming a change in the ability of the population to disperse led to a large effect: Some fish became accessible in the coastal waters, therefore increasing the landings for range‐limited fishers, but the discard rate of fish also increased, greatly impairing the long‐term biomass levels. Our evaluation, however, confirmed that no effort is displaced onto vulnerable benthic habitats and to grounds not suitable for the continued operation of fishing. We conclude that the tested spatial management is helpful, but not sufficient to ensure sustainable fishing in the area, and therefore, additional management measures should be taken. Our test platform investigates the interaction between fish and fisheries at a fine geographical scale and simulates data for varying fishing methods and from different harbor communities in a unified framework. We contribute to the development of effective science‐based inputs to facilitate policy improvement and better governance while evaluating trade‐offs in fisheries management and marine spatial planning.
Preadaptative Transactions and Institutional Change: Wolf‐critical activism in southwestern Finland
Authors: Hiedanpää Juha , Pellikka Jani 
Published: February 2017

AbstractFinland has had problems protecting the grey wolf (Canis lupus) for decades. Over the past few years, the government of Finland has taken several steps to improve its policy on wolves. In this paper, we explore the grassroots activism that institutional adjustments have triggered. This work builds on ethnographic presence in two southwestern Finnish wolf territories. Theoretically, we draw from institutional theory and use transactions as the unit of analysis. We identify four ways in which wolf‐critical civil society has provided an institutional basis for particular changes: (i) questioning the purity of the wolf, (ii) making scientific evidence fallible, (iii) producing negative emotional effects and (iv) maintaining strong policy pressure against the presence of the wolf. In the discussion, we explain how these modifications have functioned. Actors critical of the wolf have exercised the politics of disturbance, produced various epistemic cues and created conditions for reframing existing rights and the emergence of new ones. Due to these bottom‐up preadaptive transactions, wolf‐policy‐related institutional adjustments have, in many respects, taken on volitional and spontaneous features. Copyright © 2017 John Wiley & Sons, Ltd and ERP Environment
The importance of myocardial contractile reserve in predicting response to cardiac resynchronization therapy
Authors: Kloosterman Mariëlle , Damman Kevin , Van Veldhuisen Dirk J. , Rienstra Michiel , Maass Alexander H. 
Published: February 2017

AbstractAimTo perform a meta‐analysis and systematic review of published data to assess the relationship between contractile reserve and response to cardiac resynchronization therapy (CRT) in patients with heart failure.Methods and resultsWe searched MEDLINE/PubMed and Cochrane for all papers published up to 26 April 2016, supplemented by manual searches of reference lists from retrieved articles. The search strategy yielded nine observational studies that met our eligibility criteria with a total of 767 patients of which 757 provided data for this analysis. Contractile reserve after dobutamine infusion was present in 496 patients (66%). During follow‐up 474 patients (63%) qualified as CRT responders. The presence of contractile reserve was associated with a higher chance of CRT response (odds ratio 4.42, 95% confidence interval 2.15–9.07, P < 0.001) using a random‐effects model. There was evidence of publication bias. Imputation of missing studies attenuated the association to some extent, but the positive association between contractile reserve and CRT response remained with an odds ratio of 2.42 (95% confidence interval 1.17–5.05, P = 0.018).ConclusionThe presence of global contractile reserve at baseline, as assessed by dobutamine stress echocardiography, is associated with a higher chance of CRT response in patients with heart failure.
Optimal energy‐efficient resource allocation in uplink SC‐FDMA networks
Authors: Orakzai Farooq Alam , Ahmad Ayaz , Khan Muhammad Toaha Raza , Iqbal Muhammad 
Published: February 2017

AbstractEnergy efficiency (EE) is currently the key performance evaluation metric for wireless networks. This paper considers the maximization of EE of an uplink wireless network that uses single‐carrier frequency division multiple access. The EE metric considered is bits/Joule, and a joint power and subchannel allocation problem is formulated to maximize this EE metric under constraints on the minimum achieved data rate of each user, the maximum transmit power budget of each user, and the exclusive as well as the consecutive allocation of single‐carrier frequency division multiple access subchannels among the users. This problem is a nonlinear and combinatorial optimization problem, and its optimal solution is prohibitively difficult. To make the problem tractable and find its optimal solution, it is transformed into an equivalent binary integer programming problem, which is a standard set partitioning problem. This approach named as the optimal energy‐efficient (OPT‐EE) algorithm finds optimal solution with significantly reduced computational complexity compared to the solution of the original problem. In addition, a heuristic suboptimal energy‐efficient approach is also investigated, which has acceptable performance with much less computational complexity compared to the OPT‐EE algorithm. Simulation results demonstrate the performance of OPT‐EE and heuristic suboptimal energy‐efficient algorithms and their comparison with the available work in the literature.
Efficient photocatalytic degradation of 4‐nitrophenol over graphene modified TiO2
Authors: Liu Xing , Zhao Lanhua , Lai Hua , Li Sha , Yi Zhengji 
Published: February 2017

AbstractBACKGROUNDPhotocatalytic degradation of organic compounds, including nitrophenol, is one of the most popular approaches to pollution control and disposal. Graphene‐based nanocomposites have attracted considerable attention in photocatalytic research owing to their excellent electrical, thermal and mechanical properties. In this work, TiO2 material was modified with graphene by a hydrothermal method, the photocatalytic performance of the prepared catalyst was evaluated by degradation of 4‐nitrophenol, which is a highly toxic, stable and carcinogenic organic pollutant.RESULTSThe characterization results showed that the low amount of graphene disperses well over TiO2 and it does not influence the crystal phase, but makes the specific surface area of graphene/TiO2 greater than that of TiO2. Compared with TiO2, graphene/TiO2 exhibits enhanced photocatalytic activity for the degradation of 4‐nitrophenol under simulated solar irradiation.CONCLUSIONThe enhanced activity of photo‐degradation could be attributed to the involved graphene being beneficial to charge transportation and separation of photogenerated charge carriers, and providing more surface active sites for degradation reaction of the target pollutant. The present result highlights the important application of graphene as a novel carbon material in treating highly toxic pollutant through photocatalysis technology. © 2017 Society of Chemical Industry
Towards an extractive bioconversion of 3‐hydroxypropionic acid: study of inhibition phenomena
Authors: Burgé Gregoire , Moussa Marwen , Saulou‐Bérion Claire , Chemarin Florian , Kniest Mariane , Allais Florent , Spinnler Henry‐Eric , Athès Violaine 
Published: February 2017

AbstractBACKGROUND3‐hydroxypropionic acid (3‐HP) microbial synthesis through glycerol bioconversion by Lactobacillus reuteri is at the moment characterized by too low performances to consider production at industrial scale. To avoid its toxic accumulation in the medium and to recover this molecule of interest, 3‐HP in situ reactive extraction from bioconversion broth was investigated using a hollow fiber membrane contactor (HFMC) in order to intensify its production. The so‐called integrated system was compared with the conventional bioconversion system. The impact of the extractive bioconversion on the overall production performance and on cell physiological state was studied.RESULTSResults underlined drastic inhibitory effects on the producing bacteria, especially under extractive bioconversion conditions despite the use of a HFMC supposed to avoid direct contact between organic phase and bacteria. Indeed, the extractant phase components (trioctylamine in n‐decanol) were found to be toxic for the cells (due to solubility and by direct contact). These phenomena were increased by the presence of 3‐hydroxypropionaldehyde (3‐HPA) and 3‐HP produced during glycerol bioconversion. These cumulative effects induced complete loss of the cell membrane integrity and esterase activity after 1.5 h of extractive bioconversion. When the bioconversion was conducted alone, the bacterial inhibition was lower, as around 50% of L. reuteri cells remained active with unaltered membrane after 3 h. Hypotheses concerning the mechanisms of action of the observed inhibitions were proposed and discussed.CONCLUSIONSThis work highlighted the importance of performing such an integrative approach to address specific questions prior to the optimization of the whole process. © 2017 Society of Chemical Industry
Platelet rich plasma promotes skeletal muscle cell migration in association with up‐regulation of FAK, paxillin, and F‐Actin formation
Authors: Tsai Wen‐Chung , Yu Tung‐Yang , Lin Li‐Ping , Lin Mioa‐Sui , Tsai Ting‐Ta , Pang Jong‐Hwei S. 
Published: February 2017

ABSTRACTPlatelet rich plasma (PRP) contains various cytokines and growth factors which may be beneficial to the healing process of injured muscle. The aim of this study was to investigate the effect and molecular mechanism of PRP on migration of skeletal muscle cells. Skeletal muscle cells intrinsic to Sprague–Dawley rats were treated with PRP. The cell migration was evaluated by transwell filter migration assay and electric cell‐substrate impedance sensing. The spreading of cells was evaluated microscopically. The formation of filamentous actin (F‐actin) cytoskeleton was assessed by immunofluorescence staining. The protein expressions of paxillin and focal adhesion kinase (FAK) were assessed by Western blot analysis. Transfection of paxillin small‐interfering RNA (siRNAs) to muscle cells was performed to validate the role of paxillin in PRP‐mediated promotion of cell migration. Dose‐dependently PRP promotes migration of and spreading and muscle cells. Protein expressions of paxillin and FAK were up‐regulated dose‐dependently. F‐actin formation was also enhanced by PRP treatment. Furthermore, the knockdown of paxillin expression impaired the effect of PRP to promote cell migration. It was concluded that PRP promoting migration of muscle cells is associated with up‐regulation of proteins expression of paxillin and FAK as well as increasing F‐actin formation. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2506–2512, 2017.
Dynamical analysis of dislocation‐associated factors in total hip replacements by hardware‐in‐the‐loop simulation
Authors: Geier Andreas , Kluess Daniel , Grawe Robert , Herrmann Sven , D'Lima Darryl , Woernle Christoph , Bader Rainer 
Published: February 2017

ABSTRACTSince dislocation of total hip replacements (THR) remains a clinical problem, its mechanisms are still in the focus of research. Previous studies ignored the impact of soft tissue structures and dynamic processes or relied on simplified joint contact mechanics, thus, hindered a thorough understanding. Therefore, the purpose of the present study was to use hardware‐in‐the‐loop (HiL) simulation to analyze systematically the impact of varying implant positions and designs as well as gluteal and posterior muscle function on THR instability under physiological‐like loading conditions during dynamic movements. A musculoskeletal multibody model emulated the in situ environment of the lower extremity during deep sit‐to‐stand with femoral adduction maneuver while a six‐axis robot moved and loaded a THR accordingly to feed physical measurements back to the multibody model. Commercial THRs with hard‐soft bearings were used in the simulation with three different head diameters (28, 36, 44 mm) and two offsets (M, XL). Cup inclination of 45°, cup anteversion of 20°, and stem anteversion of 10° revealed to be outstandingly robust against any instability‐related parameter variation. For the flexion motion, higher combined anteversion angles of cup and stem seemed generally favorable. Total hip instability was either deferred or even avoided even in the presence of higher cup inclination. Larger head diameters (>36 mm) and femoral head offsets (8 mm) deferred occurrence of prosthetic and bone impingement associated with increasing resisting torques. In summary, implant positioning had a much higher impact on total hip stability than gluteal insufficiency and impaired muscle function. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2557–2566, 2017.
Quantitation of Targetable Somatic Mutations Among Patients Evaluated by a Personalized Medicine Clinical Service: Considerations for Off‐Label Drug Use
Authors: Vela Cory M. , Knepper Todd C. , Gillis Nancy K. , Walko Christine M. , McLeod Howard L. , Hicks J. Kevin 
Published: February 2017

IntroductionMoffitt Cancer Center's Personalized Medicine Clinical Service (PMCS) reviews somatic next‐generation sequencing (NGS) assay results, provides interpretations, and identifies potential therapeutic options. The number of individuals reviewed by our clinical service who are eligible for on‐label or off‐label drug therapy based on genetic test results has previously not been quantitated. We determined the number of patients harboring an actionable mutation that would qualify a patient for an on‐label drug or consideration for off‐label drug treatment.MethodsThe Food and Drug Administration (FDA) Table of Pharmacogenomic Biomarkers in Drug Labeling was utilized to identify anticancer agents containing genomic markers in the Indications and Usage section of the drug label. A database containing discrete NGS patient data was queried retrospectively for those drugs and associated genomic mutations included in this study. On‐label was defined as those patients who were eligible for a drug based on harboring a targetable mutation in the FDA‐approved cancer type. Off‐label was defined as those patients who may be considered for a drug based on harboring a targetable mutation in a non–FDA‐approved cancer type.ResultsA total of 1072 patients and 1131 NGS results were eligible for study inclusion. Fifty‐two patients (4.9%) had results for more than one NGS assay. Seventeen drugs targeting ALK, BRAF, BRCA1/BRCA2, EGFR, or ERBB2 mutations met the study inclusion criteria. Of the entire patient population, 92 (8.6%) unique patients were eligible for at least one on‐label drug; off‐label use of at least one drug could be considered in 103 (9.6%) unique patients.ConclusionCombining both on‐label and off‐label opportunities, 175 (16.3%) unique patients had actionable mutations in six genes. Because most patients reviewed by our PMCS have previously treated advanced disease with limited treatment options, identifying additional lines of therapy is of clinical utility.
Induced Pluripotent Stem Cell-Derived Endothelial Cells Overexpressing Interleukin-8 Receptors A/B and/or C-C Chemokine Receptors 2/5 Inhibit Vascular Injury Response
Authors: Giordano Samantha , Zhao Xiangmin , Chen Yiu-Fai , Litovsky Silvio H. , Hage Fadi G. , Townes Tim M. , Sun Chiao-Wang , Wu Li-Chen , Oparil Suzanne , Xing Dongqi 
Published: February 2017

AbstractRecruitment of neutrophils and monocytes/macrophages to the site of vascular injury is mediated by binding of chemoattractants to interleukin (IL) 8 receptors RA and RB (IL8RA/B) C-C chemokine receptors (CCR) 2 and 5 expressed on neutrophil and monocyte/macrophage membranes. Endothelial cells (ECs) derived from rat-induced pluripotent stem cells (RiPS) were transduced with adenovirus containing cDNA of IL8RA/B and/or CCR2/5. We hypothesized that RiPS-ECs overexpressing IL8RA/B (RiPS-IL8RA/B-ECs), CCR2/5 (RiPS-CCR2/5-ECs), or both receptors (RiPS-IL8RA/B+CCR2/5-ECs) will inhibit inflammatory responses and neointima formation in balloon-injured rat carotid artery. Twelve-week-old male Sprague-Dawley rats underwent balloon injury of the right carotid artery and intravenous infusion of (a) saline vehicle, (b) control RiPS-Null-ECs (ECs transduced with empty virus), (c) RiPS-IL8RA/B-ECs, (d) RiPS-CCR2/5-ECs, or (e) RiPS-IL8RA/B+CCR2/5-ECs. Inflammatory mediator expression and leukocyte infiltration were measured in injured and uninjured arteries at 24 hours postinjury by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. Neointima formation was assessed at 14 days postinjury. RiPS-ECs expressing the IL8RA/B or CCR2/5 homing device targeted the injured arteries and decreased injury-induced inflammatory cytokine expression, neutrophil/macrophage infiltration, and neointima formation. Transfused RiPS-ECs overexpressing IL8RA/B and/or CCR2/5 prevented inflammatory responses and neointima formation after vascular injury. Targeted delivery of iPS-ECs with a homing device to inflammatory mediators in injured arteries provides a novel strategy for the treatment of cardiovascular diseases.
The Number of Point Mutations in Induced Pluripotent Stem Cells and Nuclear Transfer Embryonic Stem Cells Depends on the Method and Somatic Cell Type Used for Their Generation
Authors: Araki Ryoko , Mizutani Eiji , Hoki Yuko , Sunayama Misato , Wakayama Sayaka , Nagatomo Hiroaki , Kasama Yasuji , Nakamura Miki , Wakayama Teruhiko , Abe Masumi 
Published: February 2017

Abstract
Induced pluripotent stem cells hold great promise for regenerative medicine but point mutations have been identified in these cells and have raised serious concerns about their safe use. We generated nuclear transfer embryonic stem cells (ntESCs) from both mouse embryonic fibroblasts (MEFs) and tail-tip fibroblasts (TTFs) and by whole genome sequencing found fewer mutations compared with iPSCs generated by retroviral gene transduction. Furthermore, TTF-derived ntESCs showed only a very small number of point mutations, approximately 80% less than the number observed in iPSCs generated using retrovirus. Base substitution profile analysis confirmed this greatly reduced number of point mutations. The point mutations in iPSCs are therefore not a Yamanaka factor-specific phenomenon but are intrinsic to genome reprogramming. Moreover, the dramatic reduction in point mutations in ntESCs suggests that most are not essential for genome reprogramming. Our results suggest that it is feasible to reduce the point mutation frequency in iPSCs by optimizing various genome reprogramming conditions. We conducted whole genome sequencing of ntES cells derived from MEFs or TTFs. We thereby succeeded in establishing TTF-derived ntES cell lines with far fewer point mutations. Base substitution profile analysis of these clones also indicated a reduced point mutation frequency, moving from a transversion-predominance to a transition-predominance.
Concise Review: Mending a Broken Heart: The Evolution of Biological Therapeutics
Authors: Chen Caressa , Termglinchan Vittavat , Karakikes Ioannis 
Published: February 2017

Abstract
Heart failure (HF), a common sequela of cardiovascular diseases, remains a staggering clinical problem, associated with high rates of morbidity and mortality worldwide. Advances in pharmacological, interventional, and operative management have improved patient care, but these interventions are insufficient to halt the progression of HF, particularly the end-stage irreversible loss of functional cardiomyocytes. Innovative therapies that could prevent HF progression and improve the function of the failing heart are urgently needed. Following successful preclinical studies, two main strategies have emerged as potential solutions: cardiac gene therapy and cardiac regeneration through stem and precursor cell transplantation. Many potential gene- and cell-based therapies have entered into clinical studies, intending to ameliorate cardiac dysfunction in patients with advanced HF. In this review, we focus on the recent advances in cell- and gene-based therapies in the context of cardiovascular disease, emphasizing the most advanced therapies. The principles and mechanisms of action of gene and cell therapies for HF are discussed along with the limitations of current approaches. Finally, we highlight the emerging technologies that hold promise to revolutionize the biological therapies for cardiovascular diseases.