bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Fluorescent/Colorimetric Dual‐Signal Intelligent Detecting Platform (F/C‐BMIPs) Based on “Borate‐Molecular Imprinting” Collaborative Recognition for Visual Quantitative Detection of Rutin in Food Samples
Authors: Zhang Lei , Niu Xueying , Yan Zhenmin , Liu Chang , Zhang Yu , Wang Peiting , Wang Milan 
Published: October 2024

ABSTRACTHerein, a novel fluorescent/colorimetric dual‐signal intelligent detecting platform (F/C‐BMIPs) based on ratiometric fluorescence (BA‐N‐GQDs/rhodamine 6G) and collaborative recognition (borate‐MIPs) was developed for on‐site visual quantitative detection of rutin (RT) in food samples. Furthermore, the above detection system is transferred to the test strips, and combined with the color recognition ability of smartphones, the portable and user‐friendly visualization and quantitative detection of RT (F/C‐BMIPs method) is realized. Under optimal conditions, the assay system has a wide linear range of 0.2–10 μM (F‐BMIPs)/0.45–10 μM (F/C‐BMIPs), and 10–100 μM (F‐BMIPs and F/C‐BMIPs), a detection limit as low as 0.02 μM (F‐BMIPs)/0.056 μM (F/C‐BMIPs) (S/N = 3), highly imprinted factor (IF = 5.04), and fast fluorescence response (90 s). In addition, this method was successfully applied to the detection of RT in two real samples (raw buckwheat and Sophora japonica), and the recovery rate was 95.6%–103.5%. Therefore, this study provides a promising strategy for the on‐site rapid detection of cis‐diol‐containing flavonoids in food samples.
Blue‐Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility
Authors: Ba Fang , Zhang Yufei , Wang Luyao , Liu Wan‐Qiu , Li Jian 
Published: October 2024

AbstractSynthetic BioBricks introduce novel capabilities to manipulate genetic information, direct transcription‐translation processes, and program cellular behaviors in living organisms. To maintain the stability and functionality of synthetic BioBricks, assembled DNA fragments should be mutually compatible without inducing negative effects such as metabolic burden or cellular toxicity in host cells. However, a simple, rapid, and reliable method to evaluate BioBrick compatibility remains to be developed. In this study, we report BP (Blue/Purple, Ban/Pick) evaluation, a method utilizing chromoproteins to facilitate the identification of BioBrick compatibility in one‐pot reactions. By visualizing and quantifying the ratio of blue to purple Escherichia coli (E. coli) colonies on LB‐agar plates, we can easily validate the compatibility of desired BioBrick constructions. To demonstrate our design, we characterized BioBrick assemblies with antitoxin‐toxin pair ccdA‐ccdB, lysis protein E, or heterologous protein sfGFP. Among these, we successfully identified several compatible assemblies. We anticipate that BP evaluation will enhance biotechnological assessments of BioBrick compatibility in vivo and expand the application of chromoproteins in synthetic biology.
Impact of prior hospitalization for heart failure on clinical outcomes of patients after transcatheter aortic valve implantation with new‐generation devices: Insights from the LAPLACE‐TAVI registry
Authors: Fukutomi Motoki , Onishi Takayuki , Ando Tomo , Higuchi Ryosuke , Hagiya Kenichi , Saji Mike , Takamisawa Itaru , Iguchi Nobuo , Takayama Morimasa , Shimizu Atsushi , Shimizu Jun , Doi Shinichiro , Okazaki Shinya , Sato Kei , Tamura Harutoshi , Yokoyama Hiroaki , Takanashi Shuichiro , Tobaru Tetsuya 
Published: October 2024

AbstractBackgroundA history of hospitalization for heart failure (HHF) before transcatheter aortic valve implantation (TAVI) or surgical aortic valve replacement is associated with the prognosis of patients with severe aortic stenosis (AS). However, the impact of prior HHF on clinical outcomes after contemporary TAVI using new‐generation transcatheter heart valves (THVs) has not been thoroughly investigated.MethodsUsing data from the aLliAnce for exPloring cLinical prospects of AortiC valvE disease (LAPLACE)‐TAVI registry, we investigated 2,752 patients who underwent TAVI with new‐generation THVs with a median follow‐up of 627 days. The primary outcomes were all‐cause mortality and heart failure readmission after TAVI.ResultsPatients with a history of HHF (n = 809) showed a higher 30‐day mortality than patients without prior HHF (n = 1,943). A Kaplan–Meier analysis revealed that the prior HHF group showed a higher incidence of the primary outcome than the non‐prior HHF group (27.4% vs. 16.4%, log‐rank p = 0.001). In a Cox regression analysis, prior HHF was significantly associated with the risk of the primary outcome, even after adjusting for covariates (hazard ratio, 1.344; 95% confidence interval, 1.103–1.638; p = 0.003). A subanalysis showed that the prior HHF group with ejection fraction (EF) ≥ 50% had a higher risk of the primary outcome than the non‐prior HHF group, whereas the prior HHF group with EF < 50% had the worst outcome.ConclusionA history of prior HHF is associated with worse outcomes in patients with severe AS, both in those with preserved EF and those with reduced EF, even after TAVI using new‐generation devices.
A Practical Guide to 3D Printing for Chemistry and Biology Laboratories
Authors: Pamidi Arjun S. , Spano Michael B. , Weiss Gregory A. 
Published: October 2024

AbstractThree‐dimensional (3D) printing promises a revolution in laboratory creativity by enabling rapid prototyping, broader availability of scientific apparatuses, and transformative scientific workflows. We believe all chemistry and biology laboratories should equip themselves with one or more 3D printers and a critical mass of scientists trained to operate them. This overview surveys the techniques, intricacies, and pitfalls associated with 3D printing of functional parts, including measurements, computer‐aided design, slicing, limitations of 3D printing, troubleshooting, tips for tricky filaments, and 3D printer maintenance. A flow cells are essential tools in chemistry and biology laboratories, we discuss techniques relevant to the construction of watertight 3D‐printed parts. Finally, we articulate a set of principles required for reporting 3D‐printed innovations to improve the field's reproducibility and encourage iterative improvements by other scientists. Ideally, authors, peer reviewers, and editors will adopt these principles. We hope these protocols inspire a new generation of publications applying 3D printing in chemistry and biology—especially highly reproducible inventions with the requisite detail and associated documentation. Such reports will facilitate broad adoption and creative iteration of the most innovative designs, thus accelerating discovery in chemistry and biology. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.
A New Life for Tionite Industrial Waste: Regeneration Strategy towards Photocatalytic Applications
Authors: Viganò Lorenzo , Di Credico Barbara , Parrino Francesco , Keller Nicolas , Bellotto Maurizio , D'Arienzo Massimiliano , Mostoni Silvia , Latorrata Saverio , Dotti Anna , Nisticò Roberto , Scotti Roberto 
Published: October 2024

AbstractIndustrial waste management is an urgent problem to solve possibly by recycling, reuse and recovery of resources. In this framework, the sludge of the TiO2 manufacturing, called tionite, is of certain interest because it contains metallic oxide‐bearing impurities, dangerous to the environment. Hence, we propose a strategy to recover TiO2 from tionite, towards its effective re‐use as a photocatalyst. In detail, tionite was treated under acidic or alkaline conditions to remove impurities and improve the TiO2 accessibility, and each single purification step was monitored by a thorough multi‐technique characterization.The photocatalytic activity of the modified tionite materials was tested for the partial oxidation of ferulic acid to vanillin, a relevant sustainable green reaction, being vanillin an industrially relevant high added value compound and ferulic acid an abundant component of lignin present in industrial waste. All the tionite catalysts have shown significant efficiency in terms of vanillin selectivity respect to the benchmark TiO2 P25. In particular, the sample treated under acidic conditions showed the best performances, due to specific interaction between the organic substrates and the catalyst. Results demonstrated that an industrial waste such as tionite can be valorized by proposing a tailored regeneration strategy.
A Decoupled Modulation Strategy for Constant Voltage/Current Wireless Charging System Under High Coils Misalignment
Authors: Cheng Bing , He Liangzong 
Published: October 2024

ABSTRACTOutput fluctuation and lower transmission efficiency under load and mutual inductance variation are hot concerned in the wireless charging system for power batteries. In this paper, a decoupled tracking strategy is proposed to realize constant voltage (CV) or current (CC) output and high transmission efficiency. The circuit model of series–series compensation network is built, and corresponding transmission characteristics can be derived. First, the load and mutual inductance value can be estimated by measuring the primary electrical information, including the inverter output voltage, current, and the phase error between them. Then load‐independent voltage or current output characteristics under coils misalignment can be maintained by tracking the optimal switching frequency of inverter part. In addition, the value of output voltage or current can be adjusted flexibly for different operation occasions by the employment of pulse‐width modulation. As a result, the load‐independent output characteristics realization and output adjustment can be decoupled. Based on only the primary measuring information, the CV/CC output can be maintained via the proposed hybrid control strategy when the coils misalignment and load variation happen, increasing the robustness of whole wireless power transfer (WPT) system. Furthermore, the soft switching of all power switches in the inverter part can be realized to reduce the switching loss. Compared with previous research, the proposed wireless charging system has a higher power density and a simplified control strategy. Finally, an experimental platform with 60‐V charging voltage and 3.6‐A charging current is built to verify the feasibility of the proposed sys‐tem and control method.
An Identical Code–Data Based Nonorthogonal Multiple Access
Authors: Vasudevan S. , Stuwart S. Lenty 
Published: October 2024

ABSTRACTNonorthogonal multiple access (NOMA) is a promising technique operating with limited resources. NOMA can be executed in both power and code domains. Cyclic interleaved NOMA strategies are debated in recent literature, combining power and code domains. Identical code cyclic shift (ICCS) transmission is viewed to be a bridge between code division access and NOMA. We propose identical code–data cyclic shift NOMA (ICDCS‐NOMA) in which the code and data are cyclically shifted in distinction to simple cyclic interleaved transmission introduced in our earlier work and the ICCS transmission. The cell‐edge user in a NOMA pair engages its signal transmission using ICDCS; in contrast, the cell‐center user prefers simple cyclic interleaved transmission. The spread data stream and its cyclic shifted version are blended in ICDCS, followed by cyclic interleaving. A distinct and extreme fading channel pair is assumed with the same number of paths for the edge and center users. Diversity combining multipath signals is embraced at the edge user terminal, and frequency domain equalization (FDE)/matched filter (MF) receiver is employed at the center user. The simulation indicates the dominance of the proposed ICDCS‐NOMA transmission in terms of the attained diversity gain over the conventional, ICCS, and simple cyclic interleaved transmissions.
Discharging Performance Analysis of MXene Nano‐Enhanced Phase Change Material for Double and Triplex Tube Thermal Energy Storage
Authors: Srivastava Utkarsh , Sahoo Rashmi Rekha 
Published: October 2024

ABSTRACTThe present study numerically investigates the energy and exergy analysis of solidification of phase change materials within a double tube and triple tube latent heat storage unit using ANSYS Fluent. Double tube and triple tube thermal energy storage system's thermal characteristics are examined using MXene nano‐enhanced phase change material to determine system efficiency, discharged energy, heat transfer rate, exergy destruction, entropy generation number, exergetic efficiency, liquid fraction, solidification temperature contours. The result revealed that the double tube thermal energy storage with pure cetyl alcohol PCM has 14.76% lower discharge exergy than MXene‐based nano‐enhanced phase change material in pure solidification. In a triple tube thermal energy storage system, the solidification time for MXene‐based nano‐enhanced phase change material is impressively reduced by 54.76% compared to a double tube system using pure phase change material. At a Fourier number of 0.00672, MXene nano‐enhanced phase change material exhibits an 11.69% higher Stefan number (St) than cetyl alcohol phase change material in a double tube thermal energy storage system. At 2400 s, pure phase change material and MXene nano‐enhanced phase change material generated 3.14% and 4.88% less entropy than pure cetyl alcohol in the triple tube thermal energy storage system. During the pure solidification process in a double tube thermal energy storage system, pure cetyl alcohol experiences 7.60% higher exergy destruction compared to MXene nano‐enhanced phase change material at a solidification time of 2400 s. In a triple tube thermal energy storage system, the discharging temperature for pure cetyl alcohol phase change material is 2.92% lower than that in a double tube system. Double tube thermal energy storage with pure cetyl alcohol discharged more efficiently over 2400 s. The triple tube thermal energy storage system solidified cetyl alcohol PCM 20.83% faster than pure phase change material due to MXene nanoparticles' better thermophysical properties. Thus, MXene‐based nano‐enhanced cetyl alcohol phase change material solidifies faster per volume in a triple tube thermal energy storage latent heat system.
Breaking boundaries: Word analysis strategies that draw on students' full linguistic repertoires
Authors: Choi Minkyung 
Published: October 2024

AbstractThis paper examines the need to integrate students' full linguistic repertoires into literacy instruction in middle and high school classrooms. Traditional monoglossic approaches often neglect the linguistic assets multilingual students bring, limiting their academic potential. Drawing on translanguaging theory, this paper explores three strategies—bilingual morpheme mapping, comparative morphological analysis, and multilingual word walls. These strategies seek to enhance vocabulary instruction by utilizing students' home languages and ultimately cultivating a deeper understanding of word formation and meaning. The benefits of such approaches extend beyond multilingual students, offering all students more comprehensive vocabulary knowledge across disciplines. While the advantages of these methods are evident, the paper also identifies limitations and calls for further research to explore the long‐term impacts on literacy development. By advocating for professional learning focused on translanguaging and morphological instruction, this paper highlights the need to incorporate word strategies that enhance literacy for all students.
RNA methylase RBM15 facilitates malignant progression of colorectal cancer through regulating E2F2 in an m6A modification‐dependent manner
Authors: Zhang Huijun , Li Yuanyuan , Zhou Ying , Xu Qihua , Liao Bingling , Qiu Xiaofeng , Liu Jianfeng 
Published: October 2024

AbstractRecently, RBM15 has emerged as an oncogenic factor in a majority of tumors. However, the mechanism is unclear that accounts for how RBM15‐induces colorectal cancer (CRC) progression and it is in need of further study. We determined RBM15 expression through the UALCAN database and RT‐qPCR. The role of RBM15 in inducing the malignant and aggressive cancerous phenotype was characterized based on the results of the western blot, RT‐qPCR, CCK‐8 and transwell assays. The target genes of RBM15 were screened by LinkedOmics. m6A methylation kit was applied to analyze the methylation levels of mRNA. SRAMP website was employed to predict m6A sites of targeted mRNA. RIP, dual luciferase reporter gene and actinomycin D assay were conducted to verify the interactions between RBM15 and its targeted gene, and the presence of m6A modification site of its targeted mRNA, respectively. We confirmed the augmentation of RBM15 expression in CRC, which also has a high clinical diagnostic value for CRC. Functionally, RBM15 silencing clearly restrained malignant cellular processes in CRC cells. Mechanistically, RBM15 bound to E2F2 which increased its m6A binding and stabilized the corresponding E2F2 mRNA formation. Excessive E2F2 largely restored the repression malignant phenotype of tumor cells caused by RBM15 silencing. RBM15 regulated E2F2 in an m6A modification‐dependent manner thereby boosting malignant cellular processes in CRC. The RBM15/E2F2 axis may be a novel target for CRC therapy.
Leukotriene B4 is elevated in diabetes and promotes ventricular arrhythmogenesis in guinea pig
Authors: Corbin Andrea , Aromolaran Kelly A. , Aromolaran Ademuyiwa S. 
Published: October 2024

AbstractDiabetes (DM) patients have an increased risk (~50%) for sudden cardiac death (SCD), mostly as a result of ventricular arrhythmias. The molecular mechanisms involved remain partially defined. The potent proinflammatory lipid mediator leukotriene (LT) B4, is pathologically elevated in DM compared to nondiabetic patients, resulting in increased LTB4 accumulation in heart, leading to an increased risk for life‐threatening proarrhythmic signatures. We used electrophysiology, immunofluorescence, and confocal microscopy approaches to evaluate LTB4 cellular effects in guinea pig heart and ventricular myocytes. We have observed that LTB4 is increased in multiple mouse models (C57BL/6 J/Lepob/ob and PANIC‐ATTAC) of DM, promotes profound cellular arrhythmogenesis (spontaneous beats and early after depolarizations, EADs), and severely depresses the rapidly activating delayed rectifier K current (hERG1/IKr) density in HEK293 cells and guinea pig ventricular myocytes. We have further found that guinea pigs challenged with LTB4 displayed a significantly prolonged QT interval, and that this can be prevented with LTB4R inhibition, suggesting that preventing such LTB4R effects may be therapeutically beneficial in DM. Our data suggests that a further elucidation of LTB4 vulnerable substrates, and how this leads to ventricular arrhythmias, is likely to lead to continued improvements in management options, and the development of new therapies for prevention of SCD in DM patients.
Accuracy and precision of Volumetric Matching Micromotion Analysis (V3MA) is similar to RSA for tibial component migration in TKA
Authors: de Laat Nienke N. , Koster Lennard A. , Stoel Berend C. , Nelissen Rob G. H. H. , Kaptein Bart L. 
Published: October 2024

AbstractRadiostereometric analysis (RSA) is the current gold standard to determine implant migration, but it requires bone markers and special equipment. Therefore, we developed VoluMetric Matching Micromotion Analysis (V3MA), a software program for Computed Tomography‐based radiostereometric analysis (CT‐RSA). This study aimed to determine the accuracy and precision of V3MA in vitro compared to RSA and provide a clinical proof of concept. The accuracy (RMSE (Root Mean Squared Error)) and precision (SD (standard deviation)) of V3MA were compared to RSA. A tibial component was placed in 21 different positions within a cadaveric bone to assess accuracy. For precision, a total of 20 repeated zero‐migration examinations from 4 cadaveric bones with cemented tibial components were performed. In 6 total knee arthroplasty (TKA) patients 1 to 5 year migration was measured with V3MA and RSA. V3MA accuracy ranged between 0.02 and 0.09 mm for translations and was 0.01° for internal–external rotations. For RSA, the accuracy ranged between 0.03 and 0.09 mm for translations and was 0.09° for internal‐external rotations. V3MA precision ranged between 0.01 and 0.06 mm for translations and 0.02 to 0.07° for rotations. RSA precision ranged between 0.00 and 0.06 mm for translations and 0.04 to 0.25° for rotations. V3MA was successful in 6 clinical cases and no systematic bias was present. In conclusion, the accuracy and precision of V3MA were similar to RSA. Therefore, V3MA is a promising alternative to RSA in migration measurements of tibial components in TKA.
Effect of droplet charge density on stabilization of oil‐in‐dispersion emulsions co‐stabilized by binary mixed surfactants and nanoparticles
Authors: Zhang Wanqing , Jiang Jianzhong , Cui Zhenggang 
Published: October 2024

AbstractIonic surfactant and similarly charged nanoparticles can co‐stabilize oil‐in‐dispersion (OID) emulsions at extremely low concentrations (0.001 cmc/0.001 wt%), in which particles do not adsorb at the oil/water interface but distribute in the aqueous phase forming a dispersion. In this paper, the effect of droplet charge density on stabilization of the n‐decane‐in‐water OID emulsion was examined by using a cetyl trimethyl ammonium bromide (CTAB)/C12B (dodecyl dimethyl carboxyl betaine) binary mixture at a low fixed total concentration (0.01 mM) with varying molar fractions of CTAB. A model based on the Derjaguin‐Landau‐Verwey‐Overbeek (DLVO) theory is proposed to calculate interaction energies between droplets and between droplets and particles. It is found that the droplet charge density can be well compensated by particle concentration along the stabilization boundary, and the OID emulsion still follows the DLVO stabilization. Particles tend to surround droplets at large distances but may form a monolayer between approaching droplets at shorter distances, which significantly reduces the van der Waals attraction between droplets. In addition, the induced auxiliary droplet–particle repulsion is proportional to the number of particles per unit area of droplet surfaces, which together with the droplet–droplet repulsion ensures a large total repulsion preventing droplets from flocculation and coalescence. This work explains quantitatively the stabilization of OID emulsions, which have potential applications in emulsion products such as foods, cosmetics, pesticides, and various industrial emulsion systems. Moreover, the development of the OID emulsions represents an important advancement in green chemistry as it substantially reduces the required amounts of emulsifiers and their environmental impact after use.