bluuebunny/crossref_metadata_2025_split huggingface dataset:
ID: 1085 Sodium citrate induces apoptosis in HepG2 cell lines
Authors: Nguyen Sinh Truong , Vo Phuc Hong , Nguyen Oanh Thi-Kieu , Do Nghia Minh , Pham Phuc Van 
Published: September 2017

PURPOSES: Cancer cells were observed to increase glucose uptake and fermentation of glucose to lactate to to synthesis rapidly ATP for cell growth, survival and proliferation. Thus, inhibition of glycolysis might be useful in antitumor treatment. This phenomenon occurred even with fully functioning mitochondria, and known as Warberg effect. Sodium citrate, an inhibitor of Warberg effect, was reported to antiproliferate many cancer cells line. However, sodium citrate has not been studied in Hepatocellular Carcinoma cells line yet. Here we aimed to investigate the effect of sodium citrate in HepG2 cells line.

MATERIAL AND METHODS: HepG2 cell lines was treated with sodium citrate at different concentrations. Viable cells were determined by Alamar Blue. The apoptosis induced-cells was detected by Annexin V with FCM technique. Disintegrated nuclei and DNA fragmentation was analyzed. The activity of caspase-3 was also tested.

RESULTS: We observed that the IC50 value of sodium citrate on HepG2 is at 10mM. FCM analysis showed that sodium citrate induced apoptosis in HepG2 cell line in dose-dependent manner. At 10mM sodium citrate, the caspase-3/7 was observed to be activated in time-dependent manner. Sodium citrate also induced nuclei disintergated in HepG2. DNA fragmentation was observed when HepG2 cells were treated with 10mM sodium citrate.

CONCLUSIONS: We have shown that sodium citrate possesses the antiproliferative ability on HepG2 at IC50 10mM. Sodidum citrate induces apoptosis cells in hepatocellular carcinoma HepG2 by capases-3 activation. More investigation of glycolysis inhibition of sodium citrate on HepG2 should be performed in animals
FACTORS ASSOCIATED TO THE HISTORY OF ACUTE MYOCARDIAL INFARCTION IN HYPERTENSIVE PRIMARY CARE USERS
Authors: Hafner Nascimento Fábio Simões , Cavalcante Luciana Ricarte , Zarife André Sant'Anna , Brito Luciara Leite , Silva Clara Dominguez , Maia Helena Fraga 
Published: September 2017

Objective: To investigate the factors associated to the history of acute myocardial infarction (AMI) in hypertensive patients attended at Primary Healthcare (PHC) units. Methods: A cross-sectional study was performed in a health district in the city of Salvador, Bahia, between May and September, 2013. Primary data was collected through trained interviewers. The magnitude of the association was estimated using the OR calculation, adopting the CI of 95%. Subsequently, multivariate analyses were carried out using logistic regression. The project was approved by CEP/UNEB (CAAE 09656012.0.0000.0057) and financed by PRO/PET-Saúde, 2012. Results: There were 297 hypertensive patients and, among these, 243 (81.3%) were of the female gender and had average age of 56 years (± 11.54). It was observed that 35 (11.8%) reported having suffered AMI. The risk factors associated the prior AMI were concomitant Diabetes Mellitus (DM) (OR=2.19; CI95% 1.03 – 4.68) and not being aware of the existence of health education groups (OR=5.31; CI95% 1.39 – 20.20); while the protection factors were absence of central obesity (OR=0.43; CI95% 0.20 – 0.95), taking part in health education groups (OR=0.19; CI95% 0.05 – 0.73) and having had an electrocardiogram during the past 06 months (OR= 0.37; CI95% 0.15 – 0,92). Conclusion: A high percentage of hypertensive patients with a background of AMI which was associated to the diagnosis of DM, central obesity, not having had periodic electrocardiograms and not having participated in health education groups. Strategies for the promotion of healthcare with the population and outpatient care may be considered as effective actions for the prevention of AMI.
Linked symbiotic populations: analysis of polymorphism in nfr5 receptor gene by using molecular doking
Authors: Porozov Yuri B , Muntyan Aleksey N , Chizhevskaya Elena P , Simarov Boris V , Andronov Evgeniy E 
Published: September 2017

In the present work a molecular genetic analysis of the receptor part of the nfr5 gene in 15 plants of the conjugate collection (the plant component Melilotus albus, the bacterial component Sinorhizobium meliloti) has been done. Was studied the LysM domains structure and found a high level of the nucleotide polymorphism in LysM domains of this gene. A clear relationship of nucleotide and amino acid polymorphism in the receptor region of the nfr5 gene with the genetic diversity of the rhizobial component (bacterial symbiotic locus nodD) was observed. Based on these data, the computer modeling of binding of the bacterial Nod-factor with two different variants of the plant receptor was performed.
Novel series os pea symbiotic mutants induced in the SGE line
Authors: Tsyganov Viktor E , Voroshilova Vera A , Rozov Sergey M , Borisov Aleksey U , Tikhonovich Igor A 
Published: September 2017

Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of Мsub2/sub progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fixsup–/sup phenotype), 13 mutantsunable to form nodules (Nodsup–/sup phenotype), and 2 mutants forming a few nodules (Nodsup+/–/sup phenotype). For 1 Nodsup–/sup and 5 Fixsup–/sup mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fixsup– /supmutant SGEFixsup–/sup–9 an additional mutation leading to Nodsup+/–/sup phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFixspan style="font-size:11px"sup-/sup - /span5 line is caused by a mutation in the sym33 gene, of theSGEFixsup–/sup–6 linein the sym40 gene, of the SGEFixsup–/sup–7 line in the sym27 gene, and of the SGEFixsup–/sup–8 linein the sym25 gene.
Response of immunocompetent cells of bone marrow and spleen of mouse males of several strains to stress and to pyrazine containing chemosignals
Authors: Daev Eugene V , Surinov Boris P , Dukelskaya Anna V 
Published: September 2017

The quantity of antibody producing cells and mitotic disturbances in dividing bone marrow cells of mice were studied after exposure of animals to a physical stressor or various pyrazinecontaining chemosignals. Several different strains of mice were used. We demonstrate that immune suppression and destabilization of the chromosome apparatus in dividing cells depend on: а) mouse genotype and b) side chains position in the pyrazine ring. Importance of this effects in the light of wide usage of pyrazine containing substances in perfume industry, food production and pharmacology is discussed.
The new taxonomic marker of nodulation bacteria of Rhizobium genus and its evolution
Authors: Zotov Vasily S , Punina Natalia V , Khapchaeva Sofia A , Didovich Svetlana V , Melnichuk Tatyana N , Topunov Aleksey F 
Published: September 2017

The new taxonomic marker (hin-region) has been proposed, which gives possibility for Rhizobium bacteria study on “species — group of strains” level. Using this marker the groups of Rhizobium strains were determined, which could not be distinguished with other methods, and these results correlated with evolutionary similarity of the bacteria. The developed approach for creating marker systems allows to carry out effective inventory of inter- and intraspecies genetic diversity of nodulating bacteria and to evaluate perspectives of their use in agriculture. The proposed marker system was used for description of Rhizobium bacteria samples isolated from various ecological-geographical regions of Ukraine.
MICROSATELLITE POLYMORPHISM WITHIN TWO LAKE BAIKAL OILFISH SPECIES (COMEPHORUS LACEPEDE, 1801)
Authors: Teterina Veronika I , Sukhanova Lubov V , Kirilchik Sergey V 
Published: September 2017

Intraspecific genetic polymorphism of a Baikal Lake endemic, big Baikal oilfish (Comephorus baicalensis Pallas, 1776), was evaluated based on microsatellite analysis. The obtained results have compared to the results received earlier for a little Baikal oilfish (C. dybowski Korotneff, 1905). Six microsatellite loci designed for the European sculpin, Cottus gobio, were used. Big Baikal oilfish samples were tested from the three Baikal trenches (southern, middle, northern). Average values of FsubST/sub and RsubST/subindexes between the samples did not exceed 0,02 that point to weak intraspecific genetic differentiation. Big Baikal oilfish had the smaller allele variety, the greater deficit of heterozygotes and presence of low-molecular alleles in comparison with little Baikal oilfish. The values of genetic differentiation were equaled FsubST/sub = 0,138 and RsubST/sub = 0,244 for all loci between cumulative samples of little and big Baikal oilfish. Analysis of microsatellite polymorphism has showed that both a big and little Baikal oilfishes are represented by single populations. The factor promoting panmixia within the Baikal oilfish species is apparently a passive transfer of juvenile and adult fishes by water currents.
ENDOPHYTES: STRUCTURAL AND FUNCTIONAL DIVERSITY AND BIOTECHNOLOGICAL APPLICATIONS IN CONTROL OF PLANT PATHOGENS
Authors: Berg Gabriele , M?ller Henry , Zachow Christin , Opelt Katja , Scherwinski Katja , Tilcher Ralf , Ulrich Andreas , Hallmann Johannes , Grosch Rita , Sessitsch Angela 
Published: September 2017

Endophytes are an interesting group of plant-associated bacteria that live inside plants and show neutral or beneficial interaction with their host plants. The structure of bacterial communities in endophytic microenvironments of important crops (different cultivars of potato, lettuce, and sugar beet) and native plants (different bryophyte species) was analyzed by a multiphasic approach at different field sites in Europe. Interestingly, results of the cultivation-independent approaches using Single-Strand Conformation Polymorphism (SSCP) and/or Terminal Restriction Fragments Length Polymorphism (T-RFLP) of 16S rRNA genes amplified by universal as well as group-specific and functional primers revealed a high diversity and specificity of endophytic bacterial communities. The antagonistic potential of endophytic bacteria, which was determined by screening for in vitro antagonism against different pathogens (bacteria, fungi, protists, and nematodes) ranged from 5 to 43 %. An impressive, phylogenetically diverse spectrum of antagonistic strains was found. The indigenous antagonistic potential of endophytic bacteria was influenced by the plant genotype and developmental stage, the internal microenvironment, and the soil type. A screening strategy for biocontrol strains resulted in the selection of promising candidates. These strains were evaluated in greenhouse and field trials regarding their efficiency to control pathogens under in situ conditions. One product (RhizoStar®) on the basis of serratia plymuthica HRO-C48 to control Verticillium wilt on different host plants was developed. For other promising candidates likePseudomonas trivialis 3Re2-7 (B3) and serratia plymuthica 3Re4-18 (B4) a biological control strategy against the soil-borne pathogen Rhizoctonia solani will be established. These studies were funded by the Deutsche Forschungs-gemeinschaft, the Ministry of Consumer Protection, Food and Agriculture (Germany) and by private industries (KWS SAAT AG, Strawberry farm Rovershagen, and Nor-ddeutsche Pflanzenzucht Hans-Georg Lembke KG, Ho-henlieth, Germany) by grants to G.B.
RAPD analysis the genome polymorphism Actinidia Lindley, (Actinidiacea), growing in the territory of Russia
Authors: Malaeva Elena V , Ryzhova Natalia N , Molkanova Olga I , Kochieva Elena Z 
Published: September 2017

RAPD The RAPD analysis of biodiversity in Actinidia species grown in Russia has been performed. Genome variability levels of A. kolomikta, A. arguta, A. potygama species were 0.01-0.15, 0.05-0.12 and 0.02-0.07, respectively. The maximum genetic distances have been determined between A. kotomiktaand A. arguta accessions (0.29-0.40). Close relationship have been shown between A. girald//, A. purpurea and A. arguta accessions which was comparable to intaspecific diversity analyzed in Actinidiaspecies. For the first time genetic diversity in Russian Actinidia cultivars has been investigated.
RAPD-analysis of Fagopyrum species and cultivars genome polymorphism
Authors: Kadyrova Guzel D , Kadyrova Fanusya Z , Ryzhova Natalia N , Kochieva Elena Z 
Published: September 2017

RAPD-analysis of biodiversity in cultivated annual buckwheat species Fagopyrum esculentum, F. tataricum and related species F. giganteum and F. cymosum has been performed. Close relationship between F. cymosum, F. giganteum и F. tataricum has been confirmed. Intraspecific genome variability of F. tataricum accessions (0,01-0,15) is lower than in F. esculentum (0,10-0,33). For the first time genetic diversity in Russian buckwheat cultivars has been investigated. The high level of genomic polymorphism in F. esculentum cultivars (0,10-0,29) that is comparable to the variability in natural populations is shown.
Evaluation of mutagenic activity of the pesticides: actara, sencor, mospilan, pencozeb, fastac in the Ames test
Authors: Karamova Nazira S , Denisova Alexandra P , Stasevski Zenon 
Published: September 2017

The mutagenic activity of five pesticides actara, sencor, mospilan, pencozeb, fastac widely used for treatment of potato plant lands in Tatarstan was tested in the Ames test. The non toxic concentrations of the pesticides determined in preliminary cytotoxicty test were used in the Ames assay. Pesticides actara, mospilan, pencozeb, fastac did not show mutagenic effect in Salmonella typhimurium TA 100 without rat liver S9 fraction. The weak mutagenic effect of herbicide sencor was established at concentration 1 ug/plate. Metabolic activation in vitro using rat liver S9 fraction decreased the mutagenic activity of sencor and did not alter the mutagenicity rate of the pesticides actara, mospilan, pencozeb and fastac.
«Ageingfeatures» analysis of IGF-1 and PGC-1 genes polymorphism
Authors: Potulova Svetlana V , Glotov Oleg S , Baranov Vladislav S 
Published: September 2017

Our goal was to investigate whether a polymorphism in IGF-1 gene (wild-type, 192 base pairs) and in the PGC-1 gene (Gly482Ser polymorphism) influence life expectancy. Different distribution of IGF-1 (CA repeats) gene polymorphism was shown. Increasing of 20/- genotype in elderly people compared with newborn group (26,7 and 44,1 %, accordantly, ϰsup2/sup = 8,57, p = 0,0034) and decreasing of 19/19 genotype (51 and 27,9%, accordantly, ϰsup2/sup = 14,815, p = 0.0001) were founded. Furthermore, it was shown different distribution of IGF-1 (CA repeats) gene polymorphism in man and woman. It was detected increasing of 19/20 genotype in newborn man compared with newborn woman (23,2 and 11,3%, accordantly), and significantly increasing of 19/20 genotype in elderly man compared with elderly woman (44,4 and 21,1%, accordantly, ϰsup2/sup = 5,009, p = 0,025). A possible role of IGF-1 gene CA-polymorphism in ageing that is suggested. The prevalent Gly482Ser polymorphism of the PGC-1 gene has not been shown to be associated with life expectancy. It was detected increasing of Gly/Gly genotype in elderly woman compared with elderly man (51,0 и 27,3%, accordantly, ϰsup2/sup = 4,063, p = 0,0438). The joint contribution of IGF-1 gene and PGC-1 gene is involved in ageing between groups of newborn and elderly people (20%, 41,8%, accordantly, ϰsup2/sup = 4,858, p = 0,0275).
Genetic identification of the yeast Saccharomyces kudriavzevii from European population
Authors: Naumov Gennadiy I 
Published: September 2017

Using genetic hybridization analysis we showed for the first time that Portuguese isolates belong to the biological species Saccharomyces kudriavzevii Naumov et al. (2000). Earlierthis species was described on Japanese isolates. Divergence of Portuguese and Japanese S. kudriavzevii populations, as well as different S. cerevisiae populations, on molecular galactose markers is discussed.
Polymorphism among Sinorhizobium meliloti isolates native to the origins of alfalfa diversity differed in soil-climate characteristics
Authors: Roumiantseva Marina L , Onishchuk Olga P , Belova Viktoriya S , Kurchak Oksana N , Simarov Boris V 
Published: September 2017

Sinorhizoboium meliloti populations native to the 4 distinct gene centers (GC) of alfalfa were explored toward the tolerance to salinity, cryptic plasmid profiles and symbiotic properties. The significant correlations detected among nodule (N) and trapped (T) isolates related to the similar or distinct populations. more than 60 % N- and 77 % T-isolates tolerant to 3,5 % NaCl; salt tolerant N-isolates formed effective symbiosis with Medicago sativa and M. truncatula significantly more often. Isolates native to the GC area next to aral Sea had possessed the reduced level of salt tolerance in comparison with the isolates originated from Central asian, North-Caucasian and Europe-Siberian GC; that, has related to adaptation processes, which have ensured their viability in extremely salted soils.img src="http://elibrary.ru/pic/1pix.gif" /
Symbiotic activity of the alfalfa rhizobia (Sinorhizobium meliloti) strains with the genetically modified transport of dicarboxylic acids
Authors: Onishchuk Olga P , Vorobyov Nikolay I , Provorov Nikolay A , Simarov Boris V 
Published: September 2017

Inactivation of genes involved in the dicarbyxylic acid transport in alfalfa rhizobia, Sinorhizobium meliloti (structural gene of succinate permease dctA and its transcriptional regulators dctBD, nifA, ntrA) resulted in the full or partial loss of Nsub2/sub-fixing activity while amplifications of these genes - in its improvement. It lead to the marked increases of N and C accumulation in alfalfa while its shoot mass was increased by a much lesser degree due to the incomplete N translocation from the roots. Factorial analysis suggested that dctABD amplification was important for improving the symbiotic efficiency in all trials while the effects of dctA, nifA and ntrA amplifications depend on the plant genotypes and growth conditions.
PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants
Authors: Stasevski Zenon , Ilinskaya Olga N 
Published: September 2017

PVYsup style="line-height:1.6em"NTN/sup-CP span style="line-height:1.6em"coat protein gene from a necrotic strain of potato virus /spanY (pvysup style="line-height:1.6em"ntn/sup) span style="line-height:1.6em"has been transferred into two potato /spanSolanum tuberosum L. span style="line-height:1.6em"cultivars /spanMindenes span style="line-height:1.6em"and /spanSomogyi kifli via Agrobacterium tumefaciens span style="line-height:1.6em"transformation. Expression of integrated PVY/spansup style="line-height:1.6em"NTN/supspan style="line-height:1.6em"-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains /span(PVY°, PVYsup style="line-height:1.6em"NTN/sup) span style="line-height:1.6em"of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains./span
Epigenetic regulation of Drosophila nonautonomous p-element transpositions at different temperatures of development
Authors: Ilyina Julia A , Varentsova Elena R , Kotlovanova Ludmila V , Konev Alexander Yu , Khromykh Yuri M 
Published: September 2017

In a system of Drosophila P-element activation at 25 °С and 18 °С we observed the increase of the gene conversion frequency among the offspring of parents containing nonautonomous P-elements and a chromosome with rad201supG1/sup mutation in genome. The similar increase of conversion events in this system was shown in the experiments with mutation mei41D5. In both cases inheritance of the increased gene conversion frequency had an epigenetic character, since increased conversion was observed also among the offspring not carrying mutations rad201supG1/sup or mei41D5.
Dynamics of the gene pool of petersburg Adalia bipunctata population during 75 years of observation
Authors: Zakharov-Gezekhus Ilya A 
Published: September 2017

Composition of Adalia bipunctata population "University" (collected near St. Petersburg University) was studied from 1975 till 2009, also considering the data obtained by J. Lusis who sampled this ladybird population in 1933, 1934, and 1947. The part of black-colored forms in the population decreased from 90.0 % in 1933 to 47.8 % in 2009. Two hypotheses explaining the observed population dynamics are suggested and discussed: ecological (effect of the city air pollution) and genetic (replacement of the black-color allele with more efficient polygenic system).
MOLECULAR-GENETIC ANALYSIS OF THE DIP1 REGULATOR GENE IN VARIOUS SPECIES OF DROSOPHILA
Authors: Nefedova Lidiya N , Korostin Dmitry O , Potapova Maria V , Romanova Natalia I , Kim Alexander I 
Published: September 2017

The DIP1 is a regulator gene of D. melanogaster with an unknown function. As a result of mRNA alternative splicing, at least 6 coding sequences are formed (DIP1-c/Klett-c, DIP1-b/ Klett-d, DIP1-d, DIP1-а, Klett-a, and Klett-b). Structure and expression of DIP1 homologues in various species of the melanogaster subgroup, such as D. melanogaster, D. sechellia, D. mauritiana, D. simulans, D. erecta,and D. yakuba, have been analyzed. In D. sechellia, D. simulans, and D. mauritiana we found alterations, which affected expression of the DIP1 homologues in contrast to D. melanogaster, D. erecta, and D. yakuba. These alterations have to do with splicing of the first alternative exon. It has been demonstrated that DIP1 homologues of D. sechellia, D. simulans and D. mauritiana do not code for Klett-а and Klett b forms.
Protein inheritance and regulation of gene expression in yeast
Authors: Mironova Ludmila N 
Published: September 2017

Prions of lower eukaryotes are genetic determinants of protein nature. Last years are marked by rapid development of the conception of prion inheritance. The list of yeast proteins, which have been shown to exist in the prion form in vivo, and phenotypic manifestation of prions provide good reason to believe that protein prionization may represent epigenetic mechanism regulating adaptability of a single cell and cellular population to environmental conditions.
Genetics or epigenetics? A peculiar case from the ciliate life
Authors: Yudin Aleksander L 
Published: September 2017

Inheritance of three mating types (MTs) in the ciliate Dileptus anser is described. When reproduced vegetatively, clones of dilepti retain their mating type invariably. In sexual generations (at crosses) the character behaves as controlled by one locus with three alleles in it, manifesting serial dominance. In other words, the character seems to be under the direct genic control. However, after treatment with Actinomycin D, ciliates from clones which stably express this or that MT, become destabilized and start to express in turn all three MTs. It suggests that actually MT of such a clone results from stable epigenetic differentiation of a complex, multipotential locus.