bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical Analysis of Risk Factors in High-Risk Penetrating Keratoplasty
Authors: Arzhimatova G. Sh. , Chernakova G. M. , Salikhov E. A. , Shemyakin M. Y. 
Published: October 2024

To identify and evaluate risk factors that contribute to the development of graft rejection after corneal transplant, and to create a predictive model for this condition in patients at high risk.Patients and methods. This study included the outcomes of penetrating keratoplasty in 120 cases (108 patients) from the Moscow City Ophthalmological Center (MCOC), “Botkin Hospital” of the Moscow Department of Health, from July 2020 to December 2021. All procedures were classified as high risk for graft disease development. The patients were followed for 12–36 months after surgery. To assess the relationship between transplantation outcomes (loss of transparency/engraftment) and other factors, local and general health conditions were evaluated, as well as donor material characteristics. Kaplan-Meyer and Cox regression methods were used to analyze graft survival and risk factors for graft disease.Results. The multivariate analysis revealed several significant indicators of development graft disease. Among these, the following factors were found to significantly increase the risk of graft failure: a high level of vascularization in the recipient’s eye, a history of ophthalmological or systemic conditions, the size and location of the pathology in the cornea, and the presence of an identified pathogen. Based on these findings, a new method for predicting graft failure in patients undergoing penetrating corneal transplantation has been proposed (RF Patent No. 2023109334, issued on April 13, 2023).Conclusion. In assessing the risk factors for corneal graft disease during high-risk operations, factors of the local and general somatic status of the operated eye turned out to be significant. The proposed method is simple, accessible and practical for assessing the prospects for transparent engraftment in patients in the high-risk keratoplasty group.
Significance of Biomarkers of Endothelial Dysfunction in Central Retinal Vein Occlusion
Authors: Belskaya K. I. , Moshetova L. K. , Kazakov S. P. 
Published: October 2024

Retinal vein occlusion is a severe vascular pathology of the retina, leading to a significant decrease in vision and blindness. Fundus lesion manifests itself as cystic edema of the macular region, long-lasting persistent macular edema is a poor prognostic sign.The purpose: to analyze the relationship between the clinical course of retinal vascular occlusion and the content of VEGF and MCP-1 in lacrimal fluid. The content of MCP-1 lacrimal fluid and VEGF lacrimal fluid was studied in 3 groups — in group I (patients with the central retinal vein occlusion, in group II (patients with the central retinal artery occlusion), in the control group (practically healthy individuals). The clinical course data are presented by data on best corrected visual acuity (BCVA) in patients of groups I and II and data on retinal thickness in fovea centralis (according to the results of OCT fovea centralis) in patients of group I. Statistical analysis showed no correlation between the studied biomarkers. The results showed a significant correlation of BCVA with retinal thickness in fovea centralis and a significant correlation with lacrimal fluid VEGF in group I patients, as well as a moderate correlation between lacrimal fluid VEGF and retinal thickness in fovea centralis.
Glucocorticosteroids and Postradiation Macular Edema: Rationale for Choice of Therapy and Efficacy of Use
Authors: Panova I. E. , Bikhovsky A. A. , Samkovich E. V. , Svistunova E. M. 
Published: October 2024

Purpose. To substantiate and study the effectiveness of intravitreal injection of dexamethasone implant (“Ozurdex”) based on OCT study of morphostructural biomarkers of post-radiation macular edema (PMO) in different terms of its detection.Patients and methods. 28 patients with PMO after Ru-106 ± Rh-106 (BT) brachytherapy for choroidal melanoma (CM). Study group (IG 1) — 18 patients with PMO detected up to 9 months after BT, IG 2 — 10 patients with PMO detected 9 months or more after BT. OCT parameters studied: height of foveolar and parafoveolar zones, presence of neuroepithelium detachment, disorganization of inner layers, hyperreflective foci, pigment epithelium atrophy, as well as the form of edema (diffuse, cystic) and ellipsoid zone disorder. Intravitreal injections of dexamethasone implant (“Ozurdex”) were used as drug therapy of PMO.The results showed that OCT morphostructural changes such as neuroepithelial detachment were significantly more frequent in patients with PMO diagnosed before 9 months, while hyperreflective foci, disorganization of the inner retinal layers, and ellipsoid zone disorder were more frequent in the comparison group. At the same time, there was no statistically significant difference in the macular and parafoveal edema height, frequency of edema form and presence of such a sign as pigment epithelium atrophy in both groups. Evaluation of the efficacy of intravitreal injection of “Ozurdex” implant in both groups showed that after 1–2 months from the beginning of treatment in the group with early detection of PMO complete regression was obtained in all patients, while in the second studied group complete regression occurred only in one third of patients. The regression of PMO was accompanied by improvement of BCVA in the first group from 0.4 to 0.7, in the second group — from 0.3 to 0.6. Out of 28 patients, 3 patients required re-injection of the implant in average 11 months after the first injection.Conclusion. The OCT biomarkers of PMO should be considered in treatment planning and subsequent dynamic follow-up. Early diagnosis of PMO, as well as the use of intravitreal dexamethasone implant (“Ozurdex”) determines high efficiency of treatment and maximum visual rehabilitation of patients.
Dry Eye Syndrome in the Practice of a Cataract Surgeon: A Clinical Case
Authors: Maychuk N. V. , Petrova M. G. , Yarkin D. A. , Sarhadov N. Sh. 
Published: October 2024

Relevance. Dry eye syndrome is an important medical and social problem due to its high prevalence and impact on visual functions in human daily life. In cataract surgery, the result of the operation and the patient’s satisfaction directly depends on the preoperative examination, in which, as a rule, the indicators of tear production and the condition of the tear film are most often not taken into account. In this regard, the calculation of the intraocular lens may be incorrect.Purpose. To evaluate the effectiveness of an integrated approach in preparing a patient for cataract surgery with the implantation of a “premium” lens and full recovery in the postoperative period.Materials and methods. A patient applied to YourMed and underwent a standard examination for a refractive patient, including a comprehensive assessment of the condition of the ocular surface. Based on the results of the identified changes, complex therapy was proposed and EDOF IOL with an elongated wavefront was performed. When conducting a study of the condition of the ocular surface using a slit lamp with a module for diagnosing dry eye syndrome (MediWorks, China), signs of an evaporative form of mild (right eye) and moderate (left eye) degree were revealed: the average tear film rupture time was 9.68 (OD) and 2.83 (OS) seconds, the height of the lacrimal meniscus is 0.22 mm (OD) and 0.17 mm (OS), the thickness of the lipid layer is grade 3 (OD) and grade 2 (OS), the percentage of non-functioning meibomian glands is 35 % (OD) and 44 % (OS). In this regard, a comprehensive treatment of SSG has been prescribed (eyelid self-massage, instillation of drops). Two weeks after the therapy start and against its background, the patient again underwent a repeated examination of the condition of the ocular surface, in which positive dynamics was noted. A sequential uncomplicated surgery was performed to replace the lens with an intraocular IOL with an elongated wavefront, first to the left, and after 1 week to the right eye. The patient does not notice any discomfort (dry eyes, watery eyes) that were present before the start of therapy before surgery.Conclusion. Diagnosis of dry eye syndrome, differentiation of the form of this pathology and mandatory complex therapy before lens replacement surgery plays an important role in cataract surgery, which allows you to obtain stable indicators of visual functions in a shorter period after surgery.
Heightened occurrence of psychogenic non epileptic seizures during the pandemic
Authors: K P Divya , Cherian Ajith , Paramasivan Naveen Kumar , S Krishna 
Published: October 2024

Psychogenic nonepileptic seizures (PNES) are known to be frequently precipitated or aggravated by stressors.: To analyse the occurrence of PNES during the corona virus pandemic and compare data with the pre-pandemic period and identify possible aggravating factors.: In this cross-sectional comparison study, subjects with PNES attending neurology services of a teaching hospital over a 6-month period were compared against baseline in the pre-COVID period. PNES was documented by video-electroencephalography (video-EEG) or home video recording. Demographics, clinical features, frequency of PNES, presence of psychiatric comorbidity, as well as anxiety [by Hospital Anxiety and Depression Scale (HADS-A)], depressive symptoms (HADS-D) and sleep quality by Pittsburgh sleep quality index (PSQI) were tabulated.: Of the 1350 patients seen during the pandemic (6 months), 26 patients (65% female; age-26.7 years [range=4-68]) were identified to have PNES as compared to 49 of 7125 (71% female; age 28.9 years [range=5-63]) seen in the pre-pandemic period showing 2.83 times increase. HADS-A or HADS-D was abnormal (&#62;11) in 76.9 % while 80.77% had a score &#62;5 on PSQI indicating poor sleep quality. Poor sleep quality (p&#60; 0.005) and higher anxiety scores (p &#60; 0.001) correlated with PNES. : The frequency of PNES was 2.83 times high during the pandemic as compared to pre-pandemic period. Concurrent anxiety and depression increase the risk of PNES.
Intravenous thrombolysis in acute Hemi-chorea hemi-ballismus syndrome
Authors: Shah Gaurav 
Published: October 2024

Hyperkinetic movement disorders of acute onset are rare manifestation of acute ischemic stroke.They are believed to be due to lesion in basal ganglia specifically to sub thalamic nuclei. Intravenous thrombolysis in this cases can prevent major disability. Here is a case of a 32 years old lady who had sudden onset of involuntary movements of left upper and lower limb with weakness of left side of the body. She was brought to the hospital within one hour of onset of her symptoms. Her vitals, blood sugars and CT brain plain was normal. Intravenous thrombolysis was done. She had complete resolution of symptoms in next 48 hours. MRI brain with angiography infarct in right caudate, putamen and anterior limb of internal capsule, M1 segment stenosis of right middle cerebral artery for which she was referred for carotid revascularization. Thus early identification and prompt treatment helps in prevention of disability.
Construction of a Transgenic Beauveria bassiana (Hypocreales: Cordycipitaceae) Strain Expressing the SPHvt/GNA Fusion Protein and Determination of its Toxicity to Anopheles stephensi (Diptera: Culicidae) Larvae1
Authors: Li Hongxing , Zhai Suzhen 
Published: October 2024

Abstract
Mosquitoes transmit numerous diseases, including malaria and dengue fever, making mosquito control crucial for managing the spread of these and other illnesses. Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) holds promise for the biological control of mosquito vectors but faces challenges such as low pathogenicity, lengthy infection periods, reduced longevity, and a dependence on specific environmental conditions (e.g., temperature and humidity) for spore germination, thus impacting its efficacy. To address this, a strain of B. bassiana was engineered to carry the SPHvt/GNA fusion pathogenicity protein-encoding gene. A plasmid containing the gpdA promoter, GFP gene, and SPHvt/GNA gene was constructed using molecular cloning techniques, including digestion and ligation, based on the base plasmids pBarGPE1 and pBarGFP. The engineered vector plasmid pBarGFPSPHvt/GNA was integrated into the genome of B. bassiana Bb252 through Agrobacterium tumefaciens-mediated fungal genetic transformation, resulting in B. bassiana strains with the SPHvt/GNA fusion pathogenicity protein-encoding gene. These transgenic strains were identified through fluorescence screening and confirmed at the genomic, transcriptional, and protein levels. A spore suspension of Bb252::BarGFPSPHvt/GNA was applied to Anopheles stephensi Liston (Diptera: Culicidae) larvae. The effect of pathogenicity on larval survival was assessed, and the infection process was monitored. The results indicated that the construction of a transgenic B. bassiana strain for potential management of mosquito larvae.
Canada’s Recreational Cannabis Legalization and Medical Cannabis Patient Activity, 2017–2022
Authors: Armstrong Michael J. 
Published: October 2024

Objectives. To estimate changes in medical cannabis patient activity after Canada’s recreational cannabis legalization. Methods. I used linear regressions of interrupted times series models to analyze medical cannabis patient registrations per 10 000 residents, purchases per 100 registrations, and packages per purchase in Canada’s 10 provinces between April 2017 and December 2022. I tested relationships between the recreational law’s passage in June 2018, recreational sales starting in October 2018, and the arrival of edibles and vapes in December 2019. Results. Medical patient registrations initially increased; they slowed after the law passed and started decreasing after edibles became available. Medical purchasing frequencies initially decreased; they decreased further in proportion to recreational sales but stabilized after edibles became available. Medical purchase sizes were initially stable; they began increasing after edibles became available. Conclusions. Canada saw substantial decreases in medical cannabis patient registrations, but the remaining patients stabilized their purchasing frequencies and increased their purchase sizes. Public Health Implications. Other countries might see significant changes in patient usage of their medical cannabis systems after nationwide recreational cannabis legalization. ( Am J Public Health. 2024;114(S8):S673–S680. https://doi.org/10.2105/AJPH.2024.307721 )
Countermarketing Versus Health Education Messages About Sugar-Sweetened Beverages: An Online Randomized Controlled Trial of US Adults
Authors: Grummon Anna H. , Zeitlin Amanda B. , Lee Cristina J. Y. , Hall Marissa G. , Collis Caroline , Cleveland Lauren P. , Petimar Joshua 
Published: October 2024

Objectives. To test whether countermarketing messages for sugary drinks lead to lower intentions to consume sugary drinks and less perceived weight stigma than health education messages. Methods. In August 2023, we conducted an online randomized controlled trial with US adults (n = 2169). We assessed the effect of countermarketing messages, health education messages, and neutral control messages on intentions to consume sugary drinks and perceived weight stigma. Results. Both countermarketing messages (Cohen d = −0.20) and health education messages ( d = −0.35) led to lower intentions to consume sugary drinks than control messages ( Ps < .001). However, both types of messages elicited more perceived weight stigma than control messages ( ds = 0.87 and 1.29, respectively; Ps < .001). Countermarketing messages were less effective than health education messages at lowering intentions to consume sugary drinks ( d for countermarketing vs health education = 0.14) but also elicited less perceived weight stigma than health education messages ( d = −0.39; Ps < .01). Conclusions. Countermarketing messages show promise for reducing sugary drink consumption while eliciting less weight stigma than health education messages, though they may need to be refined further to minimize weight stigma and maximize effectiveness. Clinical Trial Number. ClinicalTrials.gov NCT05953194. ( Am J Public Health. 2024;114(12):1354–1364. https://doi.org/10.2105/AJPH.2024.307853 )
Metabolic Functions of the Infrapatellar Fat Pad: Implications for Knee Health and Pathology
Authors: Chou Lee S. , Zhang James , Jildeh Toufic R. 
Published: October 2024

» Despite being historically viewed as a vestigial structure, the infrapatellar fat pad (IPFP) is now recognized as a metabolically active structure, influencing knee health through cytokine production and metabolic pathways.» With distinct anatomical regions, the IPFP contains diverse cell types including adipocytes, fibroblasts, and immune cells, influencing its functional roles, pathology, and contributions to knee disorders.» The IPFP acts as an endocrine organ by releasing adipokines such as adiponectin, leptin, and tumor necrosis factor α, regulating energy balance, immune responses, and tissue remodelling, with implications for knee joint health.» The IPFP's metabolic interactions with neighboring tissues influence joint health, clinical conditions such as knee pain, osteoarthritis, postoperative complications, and ganglion cysts, highlighting its therapeutic potential and clinical relevance.» Understanding the multifaceted metabolic role of the IPFP opens avenues for collaborative approaches that integrate orthopaedics, endocrinology, and immunology to develop innovative therapeutic strategies targeting the intricate connections between adipokines, joint health, and immune responses.
WITHDRAWN: Effective symbiosis and activation of protective antioxidant systems for increasing soybean tolerance to drought
Authors: Nyzhnyk Tetiana , Kiedrzyński Marcin , Kiedrzyńska Edyta , Kots Sergii , Zalewski Maciej 
Published: October 2024

Abstract

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Micro-fungi on medicinal plants in southern China and northern Thailand
Authors: Sun Ya-Ru , Hyde Kevin D. , Liu Ning-Guo , Jayawardena Ruvishika S. , Wijayawardene Nalin N. , Ma Jian , Zhang Qian , Alotibi Fatimah , wang yong 
Published: October 2024

Abstract

This study documents the morphology and phylogeny of micro-fungi collected from various medicinal plants in southern China and northern Thailand. Based on morphological characteristics and phylogenetic analyses, 100 species are identified and distributed in three classes (Dothideomycetes, Leotiomycetes and Sordariomycetes), 16 orders, 37 families and 67 genera. We introduce one new order, Oncopodiellales, three new families, Catenuliconidiaceae, Diplocladiellaceae and Oncopodiellaceae, one new genus, Biascospora, and 54 new species: Achroiostachys browniana, A. catenate, A. fusiformispora, Acrocalymma xishuiense, Amphisphaeria hibiscicola, Apiculospora thailandensis, Arecophila maolanensis, A. yunanensis, Barriopsis caryotae, Biascospora chishuiensis, Conioscypha synnemata, Diaporthe ervatamiae, D. kunmingensis, D. tu-chungcola, D. xishuiensis, D. hannanensis, Distoseptispora gelatinosa, D. greeniana, Gregatothecium diflugoscola, Helicosporium multiseptatum, Helminthosporium thailandicum, Kalmusia tetrastigmae, Keissleriella yunnana, Lasiodiplodia houttuyniae, L. poacearum, Leptospora houttuyniae, Lophiotrema asexualis, L. guizhouense, Melanopsamma tongrenensis, Memnoniella chiangmaiensis, Murichromolaenicola dendrobii, Neoheleiosa brownii, Neohelicascus guizhouensis, Neohelicomyces sexualis, Neohendersonia tongrenensis, Neomassaria fibraureae, Neoscytalidium dendrobii, Parabahusutrabeeja hyalina, Paramyrothecium xishuiense, Phaeosphaeria boehmeriae, P. guiyangensis, Phaeosphaeriopsis spineleae, Porodiplodia guizhouensis, Psiloglonium bambusicola, P. brownii, P. guizhouense, Roussoella panzhouensis, R. wudangensis, Seriascoma guizhouense, Sphaeropsis guiyangensis, Striaticonidium olivaceobrunnea, Tamhinispora obpyriformis, V. chiangraiensis and Virgatospora thailandica, with illustrations, discussions of their taxonomic placement, and comparisons with morphologically similar taxa. Ten new combinations are introduced: Conioscypha chiangmaiense (≡ Vanakripa chiangmaiense), C. minutiellipsoidea (≡ Vanakripa minutiellipsoidea), Conioscypha obovoidea (≡ Vanakripa obovoidea), Keissleriella acacia (≡ Pleurophoma acaciae), K. italicum (≡ Pleurophoma italica), K. ossicula (≡ Pleurophoma ossicola), K. pleurospora (≡ Phoma pleurospora), Phaeosphaeria brachylaenae (≡ Didymocyrtis brachylaenae), P. pini (≡ Didymocyrtis pini) and P. septata (≡ Didymocyrtis septata). Additionally, we report 31 new host records from medicinal plants and six new geographical records for China and Thailand. We also resolved inter-generic synonymy for three species. A reference specimen is designated for Diplocladiella taurina. Detailed descriptions and illustrations of all these taxa are provided.
The Invisible Barrier: Unraveling the Link between Air Pollution and settlement intention of floating population
Authors: Cao Dongmei , Liao Yujia , Guo Ce , Xu Xiaofan 
Published: October 2024

Abstract

This paper utilizes 2017 dynamic monitoring data of China's floating population and matches it with air pollution data from 287 prefecture-level cities to construct a Probit model. The study aims to investigate the effects and pathways of air pollution on the settlement intention of floating populations. The research findings are as follows: Firstly, air pollution significantly affects the settlement intention of floating populations, with an increase in air pollution concentration leading to a notable decrease in their intent to reside. Secondly, the impact of air pollution on the settlement intention of floating populations varies based on their personal characteristics and regional features. Those with longer years of residence, higher education levels, and better air quality in their hometowns tend to be more sensitive to air pollution. Thirdly, air pollution reduces the settlement intention of floating populations by affecting their physical health. This study enriches the relevant research on environmental factors' influence on the settlement intention of floating populations, and it provides insights for the government to formulate talent attraction policies from an environmental governance perspective.