bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unidirectional fork movement coupled with strand-specific histone incorporation ensures asymmetric histone inheritance
Authors: Wooten Matthew , Snedeker Jonathan , Nizami Zehra , Yang Xinxing , Ranjan Rajesh , Urban Elizabeth , Kim Jee Min , Gall Joseph , Xiao Jie , Chen Xin 
Published: January 2018

One Sentence SummaryDNA replication establishes asymmetric epigenomesSummaryOne of the most fundamental questions in developmental biology concerns how cells with identical genomes differentiate into distinct cell types. One important context for understanding cell fate specification is asymmetric cell division, where the two daughter cells establish different cell fates following a single division. Many stem cells undergo asymmetric division to produce both a self-renewing stem cell and a differentiating daughter cell1–5. Here we show that histone H4 is inherited asymmetrically in asymmetrically dividing Drosophila male germline stem cells, similar to H36. In contrast, both H2A and H2B are inherited symmetrically. By combining superresolution microscopy with the chromatin fiber method, we are able to study histone inheritance patterns on newly replicated chromatin fibers. Using this technique, we find asymmetric inheritance patterns for old and new H3, but symmetric inheritance patterns for old and new H2A on replicating sister chromatids. Furthermore, co-localization studies on isolated chromatin fibers and proximity ligation assays on intact nuclei reveal that old H3 are preferentially incorporated by the leading strand while newly synthesized H3 are enriched on the lagging strand. Finally, using a sequential nucleoside analog incorporation assay, we detect a high incidence of unidirectional DNA replication on germline-derived chromatin fibers and DNA fibers. The unidirectional fork movement coupled with the strand preference of histone incorporation could explain how old and new H3 are asymmetrically incorporated by replicating sister chromatids. In summary, our work demonstrates that the intrinsic asymmetries in DNA replication may help construct sister chromatids enriched with distinct populations of histones. Therefore, these results suggest unappreciated roles for DNA replication in asymmetrically dividing cells in multicellular organisms.
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis
Authors: Camus Stéphane M. , Camus Marine D. , Figueras-Novoa Carmen , Boncompain Gaelle , Amanda Sadacca L. , Esk Christopher , Bigot Anne , Gould Gwyn W. , Kioumourtzoglou Dimitrios , Perez Franck , Bryant Nia J. , Mukherjee Shaeri , Brodsky Frances M. 
Published: January 2018

AbstractGlucose Transporter 4 (GLUT4) is sequestered inside muscle and fat, then released by vesicle traffic to the cell surface in response to post-prandial insulin for blood glucose clearance. Here we map the biogenesis of this GLUT4 traffic pathway in humans, which involves clathrin isoform CHC22. We observe that GLUT4 transits through the early secretory pathway more slowly than the constitutively-secreted GLUT1 transporter and localize CHC22 to the endoplasmic-reticulum-to-Golgi-intermediate compartment (ERGIC). CHC22 functions in transport from the ERGIC, as demonstrated by an essential role in forming the replication vacuole ofLegionella pneumophilabacteria, which requires ERGIC-derived membrane. CHC22 complexes with ERGIC tether p115, GLUT4 and sortilin and down-regulation of either p115 or CHC22, but not GM130 or sortilin abrogate insulin-responsive GLUT4 release. This indicates CHC22 traffic initiates human GLUT4 sequestration from the ERGIC, and defines a role for CHC22 in addition to retrograde sorting of GLUT4 after endocytic recapture, enhancing pathways for GLUT4 sequestration in humans relative to mice, which lack CHC22.SummaryBlood glucose clearance relies on insulin-mediated exocytosis of glucose transporter 4 (GLUT4) from sites of intracellular sequestration. We show that in humans, CHC22 clathrin mediates membrane traffic from the ER-to-Golgi Intermediate Compartment, which is needed for GLUT4 sequestration during GLUT4 pathway biogenesis.
Phenotypic convergence in the brain: distinct transcription factors regulate common terminal neuronal characters
Authors: Konstantinides Nikos , Kapuralin Katarina , Fadil Chaimaa , Barboza Luendreo , Satija Rahul , Desplan Claude 
Published: January 2018

SummaryTranscription factors regulate the molecular, morphological, and physiological characters of neurons and generate their impressive cell type diversity. To gain insight into general principles that govern how transcription factors regulate cell type diversity, we used large-scale single-cell mRNA sequencing to characterize the extensive cellular diversity in the Drosophila optic lobes. We sequenced 55,000 single optic lobe neurons and glia and assigned them to 52 clusters of transcriptionally distinct single cells. We validated the clustering and annotated many of the clusters using RNA sequencing of characterized FACS-sorted single cell types, as well as marker genes specific to given clusters. To identify transcription factors responsible for inducing specific terminal differentiation features, we used machine-learning to generate a ‘random forest’ model. The predictive power of the model was confirmed by showing that two transcription factors expressed specifically in cholinergic (apterous) and glutamatergic (traffic-jam) neurons are necessary for the expression of ChAT and VGlut in many, but not all, cholinergic or glutamatergic neurons, respectively. We used a transcriptome-wide approach to show that the same terminal characters, including but not restricted to neurotransmitter identity, can be regulated by different transcription factors in different cell types, arguing for extensive phenotypic convergence. Our data provide a deep understanding of the developmental and functional specification of a complex brain structure.
Impact of integrated collaborative technologies to form a collaborative culture in construction projects
Authors: Kapogiannis Georgios , Sherratt Fred 
Published: January 2018

Purpose
Research has shown that the lack of forming team collaboration works as a barrier in the use of collaborative technologies such as Building Information Modelling. So, there is a need to investigate whether and how integrated collaborative technologies have an impact on team collaboration between stakeholders, including clients, in the planning, design and construction stages. The paper aims to discuss these issues.

Design/methodology/approach
In order to investigate this interrelationship, researchers used a survey methodology involving gathering both quantitative and qualitative data, and used regression analysis to assess the strength of impact of integrated collaborative technologies on team collaboration. For the qualitative data researchers used content analysis.

Findings
Findings show that integrated collaborative technologies impact on team collaboration by assisting the development of a collaborative culture throughout a project. This collaborative culture is deployed due to access to information by stakeholders from anywhere at any time. In this culture environment, stakeholders can share and access knowledge and awareness about the project and thus gain common ground and understanding about the project brief. In addition, integrated collaborative technologies give stakeholders the capacity to control the project process, to enhance the interaction and networking project processes as well as to pre-identify and promptly respond to project errors and uncertainties.

Originality/value
The value of this paper is to contribute in the identification and impact of collaboration culture in the architecture, engineering and construction sector.
Scholarly communication through institutional repositories: proposing a practical model
Authors: Nemati-Anaraki Leila , Tavassoli-Farahi Mina 
Published: January 2018

Purpose
Recognizing that the nature of research and scholarly communication is changing rapidly, an institutional repository (IR) is a tool for collecting, storing, accessing and disseminating scholarly communication within and without the institutions. The primary reason for establishing an IR is to increase the visibility of the institution’s research output by making it an open access with regard to copyright issues. This paper aims to propose a conceptualmodel for scholarly communication through IRs to provide an opportunity to integrate and facilitate knowledge sharing so as to enrich knowledge content and enhance global access.

Design/methodology/approach
This paper proceeds in three phases. In the first phase, the published literature on IRs and scholarly communication has been reviewed critically considering their empirical applications. Next, based on the understanding gained from the previous stage, a conceptual model for scholarly communication through IRs has been proposed. In the third phase, the proposed model has been developed and finalized toward its maturity.

Findings
Based on the literature, the authors have developed a conceptual model for understanding the factors that characterize not only the design but also the development of IRs practically. An IR appears theoretically sound, but it is a difficult task in practice because of some complexities. This paper attempts to set a theoretical model for IRs as a foundation to address the gap in the current literature for researchers, experts and librarians who create, maintain and use IRs, though it does not claim to be comprehensive.

Originality/value
A few attempts have been made to investigate scholarly communication through IRs, but this is the one of its first kind that provides a theoretical model for it. It serves as a useful starting point for those interested in knowledge management and IRs.
Exploring engagement with non-fiction collections: sociological perspectives
Authors: Knudson Sarah 
Published: January 2018

Purpose
The purpose of this paper is to build on limited understandings of how readers engage with non-fiction. Drawing from prior research and three recent case studies involving non-fiction reading, this paper considers heterogeneity in modes of reading and the central role of libraries in fostering non-fiction reading cultures.

Design/methodology/approach
Findings from three recent case studies of non-fiction reading about relationship advice; developmental disorders; and financial planning, based on qualitative interviews, participant observation and survey data, are used to assess and expand understandings of non-fiction reading and collections.

Findings
There is considerable heterogeneity in modes of non-fiction reading, and readers often appropriate non-fiction texts for purposes unintended by the authors. Both physical and online libraries function as sites where non-fiction reading can be used by a broad range of demographic groups to participate in individual or group-based resistance to structural and cultural sources of power and inequality.

Practical implications
This paper provides insight into the role and value of non-fiction collections.

Social implications
Findings speak to the value of robust funding for print and online non-fiction collections in communities and schools.

Originality/value
This paper offers new empirical and theoretical insight into how non-fiction collections are used by a range of demographic groups in community and school contexts. Sociological theories are introduced to highlight the role of non-fiction collections in facilitating social change at individual and group levels.
Doing well and doing good
Authors: Glaveli Niki , Geormas Konstantinos 
Published: January 2018

PurposeThe purpose of this paper is to deepen and expand our understanding on how strategic/market orientation (SO/MO) and a cohesive/shared social vision affect performance in the social enterprise (SE) context.Design/methodology/approachA survey was conducted in Greek SEs. Stepwise and simple regression analyses were applied in order to test the hypothesized relationships among the study variables. Additionally, Baron and Kenny’s (1986) recommendation for exploring mediating effects was followed.FindingsThe results indicate the crucial role of customer orientation dimension of SO/MO in enhancing commercial effectiveness and subsequently profitability, whilst a cohesive/shared vision was found to have positive direct associations with the social effectiveness and profitability elements of SEs performance. Moreover, the findings provide some indications for the mutually reinforcing relationship between the social and commercial aspects of SEs functioning.Originality/valueThe current paper contributes to the ongoing quest to understand the strategic management element of SEs and the challenge they face in managing rival logics. Also, it addresses the gap related to the type (mainly qualitative) and geographical concentration of SE research.
Identifying and understanding entrepreneurial decision-making logics in entrepreneurship education
Authors: Ilonen Sanna , Heinonen Jarna , Stenholm Pekka 
Published: January 2018

Purpose
It is unclear how nascent entrepreneurs make decisions during the venture creation process. The purpose of this paper is to investigate decision-making logics and their transformation over time among student entrepreneurs who aim to create new business ventures in the higher education setting.

Design/methodology/approach
The study employs the mixed methods approach through the use of survey and observation data. The longitudinal survey data comprise three surveys collected via an internet-aided tool. The constructs of causation and effectuation are measured using previously tested scales (Chandler et al., 2011). Non-participant observation data were collected during the course, focussing on the venture creation processes of four different start-ups, and were analysed thematically.

Findings
The findings show three transformation patterns – doubts in how to proceed, unwillingness to proceed, and unsatisfactory team dynamics – that led individuals towards a coping decision-making logic in which no causation or effectuation is emphasised. The findings illustrate that, despite this stage of decision-making logic, the learning process continues: Even if no new business venture is launched, entrepreneurship education can still generate learning outcomes that improve students’ understanding of entrepreneurship as well as understanding of themselves as entrepreneurs.

Originality/value
This study brings the theories of causation and effectuation into the teaching of entrepreneurship. Of particular value to scholars is the fact that the study generates new understanding of the decision-making logics during new venture creation. Accordingly, this study sheds new light on the transformation and complementarity of the decision-making logic of an individual as new ventures emerge in an educational context reflecting the real-life start-up context.
Can less be more? Mentoring functions, learning goal orientation, and novice entrepreneurs’ self-efficacy
Authors: St-Jean Etienne , Radu-Lefebvre Miruna , Mathieu Cynthia 
Published: January 2018

PurposeOne of the main goals of entrepreneurial mentoring programs is to strengthen the mentees’ self-efficacy. However, the conditions in which entrepreneurial self-efficacy (ESE) is developed through mentoring are not yet fully explored. The purpose of this paper is to test the combined effects of mentee’s learning goal orientation (LGO) and perceived similarity with the mentor and demonstrates the role of these two variables in mentoring relationships.Design/methodology/approachThe current study is based on a sample of 360 novice Canadian entrepreneurs who completed an online questionnaire. The authors used a cross-sectional analysis as research design.FindingsFindings indicate that the development of ESE is optimal when mentees present low levels of LGO and perceive high similarities between their mentor and themselves. Mentees with high LGO decreased their level of ESE with more in-depth mentoring received.Research limitations/implicationsThis study investigated a formal mentoring program with volunteer (unpaid) mentors. Generalization to informal mentoring relationships needs to be tested.Practical implicationsThe study shows that, in order to effectively develop self-efficacy in a mentoring situation, LGO should be taken into account. Mentors can be trained to modify mentees’ LGO to increase their impact on this mindset and mentees’ ESE.Originality/valueThis is the first empirical study that demonstrates the effects of mentoring on ESE and reveals a triple moderating effect of LGO and perceived similarity in mentoring relationships.
Teacher perceptions of empowerment and promotion during reforms
Authors: Avidov-Ungar Orit , Arviv-Elyashiv Rinat 
Published: January 2018

Purpose
The purpose of this paper is to describe the relationship between teachers’ professional role, their sense of empowerment, and their attitudes toward managerial promotion (career development) following the implementation of educational forms.

Design/methodology/approach
The study was conducted in Israel in 2015 and included 663 teachers, 250 elementary school teachers and 413 middle or high school teachers. A questionnaire and statistical analyses (ANOVA, multivariate analysis, and correlations) examined the attitudes of teachers in one of four professional roles toward managerial promotion (their desire for future promotion and sense of organizational fairness). This was compared with their sense of empowerment (comprising feeling respected, professional growth, influence, autonomy, self-efficacy, and decision making), while controlling for their demographic and professional backgrounds.

Findings
Four-fifths of teachers were interested in pursuing managerial promotion and they perceived the promotion process as moderately fair. The greater teachers’ sense of empowerment, the greater their desire for future promotion and their belief in the fairness of the promotional process. Teachers currently holding a leadership position expressed the strongest sense of empowerment.

Practical implications
The study presents a multivariate model to predict teachers’ attitudes to managerial promotion on the basis of their professional role and sense of empowerment. The findings have implications for educational policy-making, particularly where there is a national focus on increasing school autonomy.

Originality/value
The findings will contribute to local and international research on teacher empowerment and career development.
Efficacy of MBA: on the role of network effects in influencing the selection of elective courses
Authors: Roy Vivek , Parsad Chandan 
Published: January 2018

PurposeThe purpose of this paper is to outline the importance of social network effects in influencing the elective (courses) selection among masters of business administration (MBA) students and its role in influencing the efficacy of MBA. As such, given the enormous time and investment required for students to pursue an MBA and the role of electives in forming the student’s knowledge base, it is important to consider the decision process of students while selecting elective courses as they pursue their degree.Design/methodology/approachThis study explores the relevance of network characteristics in the elective selection phenomena through questionnaires administered personally to 50 MBA students from a premier b-school in India. Thereby, it seeks to link the implications with MBA efficacy.FindingsIn the sample, the authors found that social network connections in terms of homophily and heterophily (similar and dissimilar others) had the strongest association with elective course decisions. Further, the role of tie strength (the level of intensity of the social relationship between two individuals) in governing the phenomena was not confirmed.Practical implicationsThe authors discuss the linkage of elective selection phenomena with the efficacy of MBA. Specifically, the authors focus on a possible risk where network influences may prompt a student to select electives by surpassing his or her academic interests and thereby may hamper the efficacy. The authors also outline implications from the perspective of students, professors, and administrators.Originality/valueThe study is among the initial studies to present preliminary evidence on the efficacy of MBA from the perspective of elective selection phenomena and the role of social network effects.
Accessible search and the role of metadata
Authors: Beyene Wondwossen Mulualem , Godwin Thomas 
Published: January 2018

Purpose
Some of the usability and accessibility problems of search interfaces emanate from poorly organized search results, where different types of metadata are employed. Metadata is important to make well-informed selection of resources. However, putting too much of it on search interfaces could be counterproductive. Therefore, studies suggest that metadata-related decisions need to be informed by user requirements. The purpose of this paper is to explore library metadata from usability and accessibility perspectives. It identifies search-related problems users with print disability face and explores how metadata-related decisions could be tailored to improve their experience in resource discovery and access.

Design/methodology/approach
A questionnaire was posted online for two months. It targeted people with print disability who are using the audiobook app Lydhør. It was finally possible to gather responses from 113 respondents. Two open-ended questions dealing with search and metadata issues were selected for qualitative analysis.

Findings
Most respondents mentioned the Lydhør’s intolerance to spelling errors as a problem. Some suggested the use of faceted metadata, such as genres, for exploratory search to offset frustration caused by spelling errors. Most respondents indicated the importance of summaries to be shown among search results, implying their significance for lookup searches. There have been few demands related to accessibility metadata.

Originality/value
This study could be a valuable input for inclusive/universal design of library search interfaces.
Fish consumption associated with reduction of fish allergy
Authors: Mejrhit Najlae , Azdad Ouarda , El Kabbaoui Mohamed , Chda Alae , Tazi Abdelali , Bencheikh Rachid , Aarab Lotfi 
Published: January 2018

PurposeThis paper aims to examine the differences in the self-reported allergies to food, especially fish and shellfish, between children and adults, and to study the association between fish consumption and self-reported fish and shellfish allergy (FSA) according to age.Design/methodology/approachThis study is based on a survey conducted between April 2014 and December 2016 where children and adults were recruited from the general population of the Fez-Meknes region. The total studied population was 4,046 people.FindingsThe results show that the self-reported food allergy (FA) of the general population was more frequent among children (28.2 per cent) than adults (16.8 per cent), in which eggs, fish/shellfish, milk and cereals were the most common food cited. With regard to the self-reported FSA, the authors found a prevalence of 9.5 per cent in both children and adults, whereas fish species allergies were more frequent among adults than children. The most common clinical manifestations observed in this population were cutaneous reactions. The study of the association between fish consumption and FSA shows that people who consumed fish had a lower rate of FSA, especially in adults (p< 0.001).Originality/valueThe self-reported FA of the general population was more pronounced in children than adults. Regarding the self-reported FSA, the authors have shown a prevalence of 9.5 per cent in both children and adults, indicating an important sensitivity of their population to fish and shellfish. The authors have shown that consumption of fish was associated significantly with a lower rate of FSA, especially in adults.