bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response and recovery of the macrophytes Elodea canadensis and Myriophyllum spicatum following a pulse exposure to the herbicide iofensulfuron-sodium in outdoor stream mesocosms
Authors: Wieczorek Matthias V. , Bakanov Nikita , Lagadic Laurent , Bruns Eric , Schulz Ralf 
Published: October 2016

Abstract

Interest in stream mesocosms has recently revived for higher tier aquatic macrophyte risk assessment of plant protection products mainly because 1) the highest predicted environmental concentrations for the assessment of effects are frequently derived from stream scenarios, and 2) they allow an effect assessment using stream-typical pulse exposures. Therefore, the present stream mesocosm study used an herbicide pulse exposure and evaluated the responses of Elodea canadensis and Myriophyllum spicatum. Macrophytes were exposed for 24 h to 1 μg/L, 3 μg/L, 10 μg/L, and 30 μg/L of the herbicide iofensulfuron-sodium with a subsequent recovery period of 42 d. Biological endpoints were growth rates of the main, side, and total shoot length, the shoot number, the maximum root length, and the dry weight. The total shoot length was identified as the most sensitive endpoint; the growth rate of the total shoot length was inhibited by up to 66% and 45% in M. spicatum and E. canadensis, respectively. The lowest no observed effect concentrations (NOECs) were observed at day 7 and/or day 14 after herbicide treatment and were 1 μg/L for M. spicatum and 3 μg/L for E. canadensis. The no-observed-ecologically-adverse-effect concentrations (NOEAECs) were 10 μg/L and 30 μg/L for M. spicatum and E. canadensis, respectively. Such or similar mesocosm designs are useful to simulate typical stream exposures and estimate herbicide effects on aquatic macrophytes in stream systems. Environ Toxicol Chem 2017;36:1090–1100. © 2016 SETAC
Interlaboratory validation of organism recovery for use in 42-day sediment toxicity tests with Hyalella azteca
Authors: Taylor Lisa N. , Novak Lesley 
Published: October 2016

Abstract

Environment and Climate Change Canada has developed a 42-d sediment toxicity test that includes a reproduction endpoint with the freshwater amphipod Hyalella azteca. The new methodology conducts the entire exposure in sediment, in contrast to existing standardized methods whereby adults are transferred to a water-only exposure before release of their first brood at day 28. This midtest transfer to clean water was because of the results of a juvenile H. azteca recovery trial conducted in the 1990s concluding that reproductive endpoints could be biased because of low recovery of young amphipods from sediment. Using a new procedure and reduced volume of sediment, an interlaboratory recovery trial was conducted using 2-d to 5-d old H. azteca added to control sediment. A total of 29 technicians from 8 laboratories participated in the present study. The average recovery for all laboratories and all technicians was 76% (coefficient of variation [CV] = 30%). Based on an initial target recovery of at least 80%, 19 of 29 (66%) technicians met this criterion, with an average recovery for this group of 88% (CV = 8.3%). Factors that reduced recovery success included: not using a light table, technicians with minimal sediment testing experience, and the use of imported young amphipods with limited acclimation. Excluding those results, the overall average recovery, which included 17 participating technicians, increased from 76% to 88% and lowered the CV from 30% to 8.6%. Based on these results, Environment and Climate Change Canada will recommend ≥85% average recovery of young in control sediment and require ≥80% as a technician performance criterion in its new test design for the reproduction methodology. Environ Toxicol Chem 2017;36:1085–1089. © 2016 Crown in the right of Canada. Published by Wiley Periodicals Inc. on behalf of SETAC.
Research progress of long noncoding RNA in China
Authors: Xing Yu‐Hang , Bai Zhiqiang , Liu Chu‐Xiao , Hu Shi‐Bin , Ruan Meihua , Chen Ling‐Ling 
Published: October 2016

AbstractRNA is essential for all kingdoms of life and exerts important functions beyond transferring genetic information from DNA to protein. With the advent of the state‐of‐the‐art deep sequencing technology, a large portion of noncoding transcripts in eukaryotic genomes has been broadly identified. Among them, long noncoding RNAs (lncRNAs) have been emerged as a new class of RNA molecules that have regulatory potential in a variety of physiological and pathological processes. Here we summarize recent research progresses that have been made by scientists in China on lncRNAs, including their biogenesis, functional implication and the underlying mechanism of action at the current stage. © 2016 IUBMB Life, 68(11):887–893, 2016
Degradation of alkanethiol self‐assembled monolayers in mesenchymal stem cell culture
Authors: Almeida Bethany , Shukla Anita 
Published: October 2016

AbstractAlkanethiol self‐assembled monolayers (SAMs) have been used extensively in studying the role of surface functionality and geometry on stem cell differentiation; however, the effects of stem cell culture conditions on SAM stability over time are not well understood. In this work, we examined the physical and chemical changes occurring on gold (Au)‐SAM surfaces over time as a function of Au thickness. Within a narrow range of thicknesses (4, 8, and 10 nm), we observed significant differences in temporal SAM stability for a commonly utilized, hydrophilic, protein and cell repulsive oligo(ethylene) glycol alkanethiol (HS‐(CH2)11(O(CH2)2)6OH) SAM and the hydrophobic, protein adhesive hexadecanethiol (SH‐(CH2)15CH3) SAM. Within both acellular and stem cell culture conditions, 8 nm Au resulted in the most stable SAMs (∼7 days). The 4 and 10 nm Au SAMs exhibited loss in stability following 5 days at varying degradation rates, showing 4 nm Au to be the least stable. Migration of human mesenchymal stem cells seeded on SAM surfaces showed that SAM degradation rates in acellular conditions were directly correlated with the cellular migration behavior. Findings of this study can be used to develop SAM surfaces with controlled degradation rates for applications in stem cell engineering and regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 464–474, 2017.
A Content Analysis of Research on Disability: American Counseling Association Journals Between 2003 and 2013
Authors: Woo Hongryun , Goo Minkowan , Lee Myungkyung 
Published: October 2016

This content analysis provides an overview of disability articles published in 10 major American Counseling Association journals between 2003 and 2013. In addition to the number of disability articles published during this time period, other content areas such as authorship and institutional contributors; methodology, location, and application settings; target populations and sample characteristics; type of disabilities; and topics of interest are identified. Recommendations for counseling scholars to increase the scope of research on disability are provided.Este análisis de contenido proporciona una visión general de los artículos sobre discapacidad publicados en 10 revistas principales de la Asociación Americana de Consejería (American Counseling Association) entre 2003 y 2013. Además del número de artículos sobre discapacidad publicados durante este periodo, se identifican otras áreas de contenidos, como la autoría y contribuidores institucionales, la metodología, ubicación y lugares de aplicación, las poblaciones observadas y características de los grupos de muestra, los tipos de discapacidad, y los temas de interés. Se proporcionan recomendaciones para que los expertos académicos en consejería puedan ampliar el alcance de las investigaciones sobre discapacidad.
Recruiting and Assessing Recent Young Adult Latina Immigrants in Health Disparities Research
Authors: Sheehan Diana M. , Dillon Frank R. , Babino Rosa , Melton James , Spadola Christine , Da Silva Nicole , De La Rosa Mario 
Published: October 2016

The authors interviewed 4 researchers to identify facilitators in recruiting and assessing Latina immigrants. The 4 researchers recruited 530 recent Latina immigrants (ages 18–23 years) for a study of social and cultural determinants of health. Consensual qualitative research methods revealed that respondent‐driven sampling was an effective recruitment method. Fear of deportation was a barrier. Stigma about sensitive topics (e.g., sex, drug use) did not affect participation. Findings can help counselors conduct health disparities research.Los autores entrevistaron a 4 investigadores para identificar facilitadores para el reclutamiento y evaluación de inmigrantes latinas. Los 4 investigadores reclutaron a 530 mujeres latinas que habían inmigrado recientemente (edades entre 18 y 23 años) para un estudio de los factores sociales y culturales determinantes de la salud. Los métodos de investigación cuantitativa consensuados revelaron que el muestreo dirigido por las encuestadas fue un método efectivo de reclutamiento. El miedo a la deportación fue un obstáculo. El estigma asociado a temas delicados (p. ej., el sexo, el uso de drogas) no afectó a la participación. Los hallazgos pueden ayudar a los consejeros a realizar estudios sobre disparidades en la salud.
New estimate of valvuloarterial impedance in aortic valve stenosis: A cardiac magnetic resonance study
Authors: Soulat Gilles , Kachenoura Nadjia , Bollache Emilie , Perdrix Ludivine , Diebold Benoit , Zhygalina Valentina , Latremouille Christian , Laurent Stephane , Fabiani Jean‐Noel , Mousseaux Elie 
Published: October 2016

PurposeValvuloarterial impedance (ZVA), estimating left ventricle (LV) afterload, has been proposed in transthoracic echocardiography (TTE) as a predictor of mortality in aortic valve stenosis (AVS). However, its calculation differs from arterial characteristic impedance (ZC). Our aim was to apply the concept of ZC calculation to estimate ZVA from MR with carotid tonometry and to evaluate these indices through their associations with symptoms, LV diastolic function and aortic stiffness.Materials and MethodsIn 40 patients with AVS (76 ± 13 years), ZVA‐TI derived from velocity time integral and E/Ea were estimated by TTE. ZVA‐INS, based on ZC formula, calculated as the instantaneous pressure gradient to peak flow ratio and aortic compliance were estimated by using MRI at 1.5 Tesla.ResultsBoth ZVA estimates were higher in symptomatic than asymptomatic patients (707 ± 22 versus 579 ± 53 dyne.s/cm5, P = 0.031 for ZVA‐INS and 4.35 ± 0.16 versus 3.33 ± 0.38 mmHg.m2/mL, P = 0.018 for ZVA‐TI). Although they were both associated with aortic compliance (r = ‐0.45; P = 0.006 for ZVA‐INS and r = ‐0.43; P = 0.008 for ZVA‐TI) only ZVA‐INS was associated with E/Ea (r = 0.50; P < 0.001). In multivariate analysis to identify determinants of E/Ea, a model including age, mean blood pressure, LV ejection fraction, LV mass, and aortic valve area was performed (R2 = 0.41; P < 0.01). When ZVA‐INS was added to the model, its overall significance was higher R2 = 0.56 (P < 0.01) and ZVA‐INS and LV mass were the only significant determinants.ConclusionZVA‐INS was more strongly associated with diastolic dysfunction than usual parameters quantifying AVS severity. This new ZVA estimate could improve LV afterload evaluation.Level of Evidence: 1J. Magn. Reson. Imaging 2017;45:795–803.
Plasmonic nano‐slits assisted polarization selective detour phase meta‐hologram
Authors: Min Changjun , Liu Jinpeng , Lei Ting , Si Guangyuan , Xie Zhenwei , Lin Jiao , Du Luping , Yuan Xiaocong 
Published: October 2016

AbstractTraditional detour‐phase hologram is a powerful optical device for manipulating phase and amplitude of light, but it is usually not sensitive to the polarization of light. By introducing the light‐metasurface interaction mechanism to the traditional detour phase hologram, we design a novel plasmonic nano‐slits assisted polarization selective detour phase meta‐hologram, which has attractive advantages of polarization multiplexing ability, broadband response, and ultra‐compact size. The meta‐hologram relies on the dislocations of plasmonic slits to achieve arbitrary phase distributions, showing strong polarization selectivity to incident light due to the plasmonic response of deep‐subwavelength slits. To verify its polarization sensitive and broadband responses, we experimentally demonstrate two holographic patterns of an optical vortex and an Airy beam at p‐ and s‐polarized light with wavelengths of 532nm, 633nm and 780nm, respectively. Furthermore, we realize an application example of the meta‐hologram as a polarization multiplexed photonic device for multi‐channel optical angular momentum (OAM) generation and detection. Such meta‐holograms could find widespread applications in photonics, such as chip‐level beam shaping and high‐capacity OAM communication.
image
Quantitative ultrashort TE imaging of the short‐T2 components in skeletal muscle using an extended echo‐subtraction method
Authors: C. A. Araujo Ericky , Azzabou N. , Vignaud A. , Guillot G. , Carlier P.G. 
Published: October 2016

PurposeTo introduce an ultrashort echo time (UTE) based method for quantitative mapping of short‐T2 signals in skeletal muscle (SKM) in the presence of fat, with the aim of monitoring SKM fibrosis.MethodsFrom a set of at least five UTE images of the same slice, a long‐
map, a fat‐fraction map, and a map of short‐T2‐signal fraction are extracted. The method was validated by numerical simulations and in vitro studies on collagen solutions. Finaly, the method was applied to image the short‐T2 signals in the leg of eight healthy volunteers.ResultsThe imaged short‐T2‐signal fractions in the collagen solutions correlated with their respective collagen concentrations (
). Short‐T2 tissues such as cortical bone and fasciae were highlighted in the resulting short‐T2 fraction maps. A significant fraction of short‐T2 signal was systematically observed in the skeletal muscle of all of the subjects
.ConclusionThe proposed method allows the quantitative imaging of short‐T2 components in tissues containing fat. By also having the fat‐fraction and
maps as outcomes, long‐T2 suppression is accomplished without requiring modifications to the basic UTE sequence. Although the hypersignal observed in the fasciae suggests that the short‐T2 signal observed in SKM might arise from interstitial connective tissue, further investigation is necessary to confirm this statement. Magn Reson Med 78:997–1008, 2017. © 2016 International Society for Magnetic Resonance in Medicine.
Fatal Injuries in a pre‐Hispanic Canary Mummy: Violence, Accident or Ritual Suicide?
Authors: Velasco‐Vázquez J. , Alberto‐Barroso V. , Delgado‐Darias T. , Santana‐Cabrera J. 
Published: October 2016

AbstractPerimortem traumas have been identified in a pre‐Hispanic Canary mummy (fifth–sixth centuries cal AD). The location and nature of the fractures in the cranium, cervical vertebrae, ribs and both legs indicate a fall from a considerable height, which would have been the cause of death. Supported by forensic anthropology criteria and historical information about the population of origin, the circumstances in which the fatal injuries might have occurred are discussed. Given the pattern of the injuries, it is debated whether they were the result of an accident or a ritual suicide, as aggression is thought to be a less likely scenario. Copyright © 2016 John Wiley & Sons, Ltd.
Penetrating Spinal Injury: An Extraordinary Case of Survival in Early Neolithic Siberia
Authors: Lieverse A. R. , Cooper D. M. L. , Bazaliiskii V. I. 
Published: October 2016

AbstractThis paper presents the first archaeological case of long‐term survival following a penetrating spinal injury. The afflicted young adult male individual was recovered from an Early Neolithic hunter‐gatherer cemetery in the Baikal region of Siberia (Russian Federation), dating back nearly 8000 years. The second or third lumbar vertebra exhibits a lithic projectile point embedded in the right lamina, extending most of the way through the vertebral foramen, with evidence of extensive, if not complete, healing indicating survival for at least several months. Micro‐computed tomography imaging is used to non‐destructively visualize both the point and the bone in order to better understand the nature of the traumatic event, as well as the immediate and long‐term complications associated with this grave injury. Copyright © 2016 John Wiley & Sons, Ltd.
Step‐growth thiol–thiol photopolymerization as radiation curing technology
Authors: Feillée Noémi , De Fina Maurizio , Ponche Arnaud , Vaulot Cyril , Rigolet Séverinne , Jacomine Leandro , Majjad Hicham , Ley Christian , Chemtob Abraham 
Published: October 2016

ABSTRACTThis report introduces a novel UV‐curing technology based on thiol–thiol coupling for polydisulfide network formation. Beginning with a model tris(3‐mercaptopropionate) trithiol monomer and xanthone propionic acid‐protected guanidine as photobase generator, a comprehensive characterization based on spectroscopic techniques supports the reaction of thiols into disulfides without side reactions. The best experimental conditions are described as regards to film thickness, irradiance, emission wavelength, and atmosphere composition. The results shed light on a step‐growth photopolymerization mechanism involving two steps: first, the formation of thiyl radicals by thiolate air oxidation or/and thiol photolysis, and second, their recombination into disulfide. By varying thiol functionality and structure, oligomer chain length and monomer/oligomer ratio, the network architecture can be finely tuned. The molecular mobility of the polydisulfide network is crucial to high thiol conversion rates and yields as revealed by 1H T2 NMR relaxation measurements. Ultimately, spatial control enables the formation of a photopatterned poly(disulfide) film, used as next‐generation high refractive index photoresist. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 117–128.
Structural characterization of a vegetable oil‐based polyol through liquid chromatography multistage mass spectrometry
Authors: Li Jiaxiao , Zhu Marcel 
Published: October 2016

ABSTRACTA commercial vegetable oil‐based polyol for rigid polyurethane foams has been characterized by liquid chromatography‐electrospray ionization‐quadrupole ion trap mass spectrometry (LC‐ESI‐QIT‐MS). The absolute molecular weight (MW = 960) was measured by gel permeation chromatography (GPC) equipped with both refractive index (RI) detector and static laser light‐scattering detector (SLSD), which allowed further analysis by LC‐MS. The oligo‐polyol mixture was first separated in two elutes and then investigated by a deep multistage mass spectrometry (MSn) study and completed using NMR. The major constituents identified were regioisomers of propoxylated sucrose (nPO = 6–12), and the related esters of C16:0, C18:1, and C18:2 fatty acids had a mass ratio of 6:3:1. A comparison of fatty acids composition between the sample and palm oil demonstrated that the sample was initially prepared from the mixture of sucrose and palm oil by direct propoxylation. The MSnfragmentation studies validated the structure of propoxylated sucrose and the related fatty acids derivatives. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem.2017,55, 255–262
Oncologists' communication about end of life: the relationship among secondary traumatic stress, compassion satisfaction, and approach and avoidance communication
Authors: Granek Leeat , Nakash Ora , Cohen Michal , Ben‐David Merav , Ariad Samuel 
Published: October 2016

AbstractBackgroundOncologists must communicate effectively with patients and their families about end of life (EOL). Despite the importance of communicating on this topic, many oncologists avoid these conversations. The objective of this study was to examine the associations between secondary traumatic stress and compassion satisfaction and approach and avoidant communication about EOL with cancer patients.MethodsA convenience sample of 79 oncologists (n = 27 men, n = 52 women) participated in the study. Oncologists completed a survey that included a sociodemographic and clinical information questionnaire, the Professional Quality of Life Scale, and Communication about End of Life Survey. To examine the effect of secondary traumatic stress and compassion satisfaction on approach and avoidant communication, while controlling for gender and age, 2 hierarchical linear regression analyses were computed.ResultsOncologists reported high levels of secondary traumatic stress and high compassion satisfaction. Scores on the approach and avoidant communication scales were in the mid‐range of the scale. Lower reports of secondary traumatic stress and higher compassion satisfaction were associated with higher approach communication strategies: however, only higher secondary traumatic stress was associated with higher avoidant communication strategies.ConclusionsOur findings indicate that there is an association between emotional factors and approach communication. The findings have clinical implications in designing effective communication skills training. Further research and training should take secondary traumatic stress and compassion satisfaction into consideration to be able to ensure that terminal patients and their families receive the best quality EOL care.
High‐Content Screening Identifies Src Family Kinases as Potential Regulators of AR‐V7 Expression and Androgen‐Independent Cell Growth
Authors: Szafran Adam T. , Stephan Cliff , Bolt Michael , Mancini Maureen G. , Marcelli Marco , Mancini Michael A. 
Published: October 2016

BACKGROUNDAR‐V7 is an androgen receptor (AR) splice variant that lacks the ligand‐binding domain and is isolated from prostate cancer cell lines. Increased expression of AR‐V7 is associated with the transition from hormone‐sensitive prostate cancer to more advanced castration‐resistant prostate cancer (CRPC). Due to the loss of the ligand‐binding domain, AR‐V7 is not responsive to traditional AR‐targeted therapies, and the mechanisms that regulate AR‐V7 are still incompletely understood. Therefore, we aimed to explore existing classes of small molecules that may regulate AR‐V7 expression and intracellular localization and their potential therapeutic role in CRPC.METHODSWe used AR high‐content analysis (AR‐HCA) to characterize the effects of a focused library of well‐characterized clinical compounds on AR‐V7 expression at the single‐cell level in PC3 prostate cancer cells stably expressing green fluorescent protein (GFP)‐AR‐V7 (GFP‐AR‐V7:PC3). In parallel, an orthogonal AR‐HCA screen of a small interfering (si)RNA library targeting 635 protein kinases was performed in GFP‐AR‐V7:PC3. The effect of the Src‐Abl inhibitor PD 180970 was further characterized using cell‐proliferation assays, quantitative PCR, and western blot analysis in multiple hormone‐sensitive and CRPC cell lines.RESULTSCompounds that tended to target Akt, Abl, and Src family kinases (SFKs) decreased overall AR‐V7 expression, nuclear translocation, absolute nuclear level, and/or altered nuclear distribution. We identified 20 protein kinases that, when knocked down, either decreased nuclear GFP‐AR‐V7 levels or altered AR‐V7 nuclear distribution, a set that included the SFKs Src and Fyn. The Src‐Abl dual kinase inhibitor PD180970 decreased expression of AR‐V7 by greater than 46% and decreased ligand‐independent transcription of AR target genes in the 22RV1 human prostate carcinoma cell line. Further, PD180970 inhibited androgen‐independent cell proliferation in endogenous—AR‐V7—expressing prostate cancer cell lines and also overcame bicalutamide resistance observed in the 22RV1 cell line.CONCLUSIONSSFKs, especially Src and Fyn, may be important upstream regulators of AR‐V7 expression and represent promising targets in a subset of CRPCs expressing high levels of AR‐V7. Prostate 77:82–93, 2017. © 2016 Wiley Periodicals, Inc.