bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and Biomarker Study of Bevacizumab for Hearing Loss Resulting From Neurofibromatosis Type 2–Associated Vestibular Schwannomas
Authors: Blakeley Jaishri O. , Ye Xiaobu , Duda Dan G. , Halpin Chris F. , Bergner Amanda L. , Muzikansky Alona , Merker Vanessa L. , Gerstner Elizabeth R. , Fayad Laura M. , Ahlawat Shivani , Jacobs Michael A. , Jain Rakesh K. , Zalewski Christopher , Dombi Eva , Widemann Brigitte C. , Plotkin Scott R. 
Published: March 2016

Purpose Neurofibromatosis type 2 (NF2) is a tumor predisposition syndrome characterized by bilateral vestibular schwannomas (VSs) resulting in deafness and brainstem compression. This study evaluated efficacy and biomarkers of bevacizumab activity for NF2-associated progressive and symptomatic VSs. Patients and Methods Bevacizumab 7.5 mg/kg was administered every 3 weeks for 46 weeks, followed by 24 weeks of surveillance after treatment with the drug. The primary end point was hearing response defined by word recognition score (WRS). Secondary end points included toxicity, tolerability, imaging response using volumetric magnetic resonance imaging analysis, durability of response, and imaging and blood biomarkers. Results Fourteen patients (estimated to yield > 90% power to detect an alternative response rate of 50% at alpha level of 0.05) with NF2, with a median age of 30 years (range, 14 to 79 years) and progressive hearing loss in the target ear (median baseline WRS, 60%; range 13% to 82%), were enrolled. The primary end point, confirmed hearing response (improvement maintained ≥ 3 months), occurred in five (36%) of 14 patients (95% CI, 13% to 65%; P < .001). Eight (57%) of 14 patients had transient hearing improvement above the 95% CI for WRS. No patients experienced hearing decline. Radiographic response was seen in six (43%) of 14 target VSs. Three grade 3 adverse events, hypertension (n = 2) and immune-mediated thrombocytopenic purpura (n = 1), were possibly related to bevacizumab. Bevacizumab treatment was associated with decreased free vascular endothelial growth factor (not bound to bevacizumab) and increased placental growth factor in plasma. Hearing responses were inversely associated with baseline plasma hepatocyte growth factor (P = .019). Imaging responses were associated with high baseline tumor vessel permeability and elevated blood levels of vascular endothelial growth factor D and stromal cell–derived factor 1α (P = .037 and .025, respectively). Conclusion Bevacizumab treatment resulted in durable hearing response in 36% of patients with NF2 and confirmed progressive VS-associated hearing loss. Imaging and plasma biomarkers showed promising associations with response that should be validated in larger studies.
Dlc1 interaction with non-muscle myosin heavy chain II-A (Myh9) and Rac1 activation
Authors: Sabbir Mohammad G. , Dillon Rachelle , Mowat Michael R. A. 
Published: March 2016

ABSTRACT
The Deleted in liver cancer 1 (Dlc1) gene codes for a Rho GTPase-activating protein that also acts as a tumour suppressor gene. Several studies have consistently found that overexpression leads to excessive cell elongation, cytoskeleton changes and subsequent cell death. However, none of these studies have been able to satisfactorily explain the Dlc1-induced cell morphological phenotypes and the function of the different Dlc1 isoforms. Therefore, we have studied the interacting proteins associated with the three major Dlc1 transcriptional isoforms using a mass spectrometric approach in Dlc1 overexpressing cells. We have found and validated novel interacting partners in constitutive Dlc1-expressing cells. Our study has shown that Dlc1 interacts with non-muscle myosin heavy chain II-A (Myh9), plectin and spectrin proteins in different multiprotein complexes. Overexpression of Dlc1 led to increased phosphorylation of Myh9 protein and activation of Rac1 GTPase. These data support a role for Dlc1 in induced cell elongation morphology and provide some molecular targets for further analysis of this phenotype.
Establishment and evaluation of a transgenic mouse model of arthritis induced by overexpressing human tumor necrosis factor alpha
Authors: Li Ge , Wu Yu'e , Jia Huanhuan , Tang Lu , Huang Ren , Peng Yucai , Zhang Yu 
Published: March 2016

ABSTRACTTumor necrosis factor alpha (TNFα) plays a key role in the pathogenesis of rheumatoid arthritis (RA). Blockade of TNFα by monoclonal antibody has been widely used for the therapy of RA since the 1990s; however, its mechanism of efficacy, and potential safety concerns of the treatment are still not fully understood. This study sought to establish a transgenic arthritic mouse model by overexpressing human TNFα (hTNFα) and to apply this model as a means to evaluate therapeutic consequences of TNFα inhibitors. The transgenic mouse line (TgTC) with FVB background was generated by incorporating 3′-modified hTNFα gene sequences. A progressively erosive polyarthritis developed in the TgTC mice, with many characteristics observed in human rheumatoid arthritis, including polyarticular swelling, impairment of movement, synovial hyperplasia, and cartilage and bone erosion. Gene expression analysis demonstrated that hTNFα is not only expressed in hyperplastic synovial membrane, but also in tissues without lesions, including brain, lung and kidney. Treatment of the TgTC mice with anti-hTNFα monoclonal antibodies (mAb) significantly decreased the level of hTNFα in the diseased joint and effectively prevented development of arthritis in a dose-dependent response fashion. Our results indicated that the TgTC mice represent a genetic model which can be used to comprehensively investigate the pathogenesis and therapeutics of TNFα-related diseases.
QUALITY MANAGEMENT IN DESIGN-TECHNOLOGICAL PREPARATION OF ENGINEERING PRODUCE MANUFACTURING BASED ON COMPLEX FMEA-ANALYSIS
Authors: Мирошников Вячеслав , Miroshnikov Vyacheslav , Горленко Олег , Gorlenko Oleg , Борбаць Николай , Borbats Nikolay 
Published: March 2016

Under presentday conditions the main constituent in the competitiveness of any enterprise – a capaci-ty for ensuring ever-growing requirements of a con-sumer market and concrete customers to produce quality. One of the ways to solve this not simple problem is the development and introduction of an ever-improving system of quality management (SQM) meeting the highest international requirements. A significant requirement of international standards is the application in SQM a procedure of the FMEA-analysis of kinds and consequences of failures which is used for more than half a century abroad at the creation of complex engineering systems.
To date a considerable urgency gains the introduction of the complex FMEA-methodology including the fulfillment of a product design analysis, the PFMEA-analysis of the product manufacturing method and the MFMEA-analysis of equipment (rigging). The peculiarity of such a complex analysis consists in the hierarchy and consistency of the ful-fillment various kinds of FMEA: FMEA of product – FMEA of an assembly (A) – FMEA of a part. All kinds of FMEA are connected and depend on each other. The FMEA result of product and assembly design is a basis for the FMEA of product and unit assembly. It is evident that the last should be carried out after the introduction of changes in the design of a unit according to the FMEA results of design. The FMEA of equipment should be carried out after the introduction of changes in an engineering procedure according to the FMEA results of a process. Taking into account this, first should be carried out the FMEA of a design and the FMEA of a process and the FMEA of equipment should complete the consistency of tests. The paper reports thoroughly the first two stages of the complex FMEA-analysis.
Growth Responses of Acacia mangium and Paraserianthes falcataria Seedlings on Different Soil Origin under Nursery Condition
Authors: Paramitha Tirtha Ayu , Mardji Djumali 
Published: March 2016

The objective of the present study was to examine the growth responses of Acacia mangium (mangium) and Paraserianthes falcataria (sengon) seedlings growing on different soil origin under nursery condition. This study was started in September 2012 and terminated in March 2013. The seedlings were grown from seeds sown in a plastic box filled with sterilized sands. One week after sowing, the seedlings were transplanted into polybags contained sterilized soils originated from secondary forest, Imperata cylindrica grassland and ex-coal mining. The number of all seedlings were 180 seedlings consisted of 3 different soils, 2 species of seedlings with 10 seedlings replicated 3 times. Assessment was conducted one week after transplanting, then subsequently monitored every 2 weeks, except dry weighing and counting nodules were performed at the end of the study. A completely randomized design was used in this study. The data was analyzed using Costat software. The study resulted that the different of soil origin influenced on all growth variables of mangium and sengon of 4.5 months old. The survival rate of seedlings, height and diameter increments, dry weight and root nodules were better in both species of seedlings growing on soil originated from secondary forest and Imperata grassland compared with the soil from ex-coal mining. But the survival rates of sengon seedlings were higher than that of mangium on these three soils. The highest dry weight of sengon seedlings was achieved on soil originated from secondary forest. In the present study, soil originated from secondary forest increased more in weight of shoot than root, so that the shoot-root ratio was unbalanced more than one. Based on the results of this study, it is recommended that soil from secondary forest and Imperata grassland can be used as growing media for mangium and sengon seedlings in the nursery.
Diagnosis and Monitoring of Cystinosis Using Immunomagnetically Purified Granulocytes
Authors: Gertsman Ilya , Johnson Wynonna S , Nishikawa Connor , Gangoiti Jon A , Holmes Bonnie , Barshop Bruce A 
Published: March 2016

Abstract

BACKGROUND
Cystine determination is a critical biochemical test for the diagnosis and therapeutic monitoring of the lysosomal storage disease cystinosis. The classical mixed-leukocyte cystine assay requires prompt specialized recovery/isolation following blood drawing, providing cystine concentrations normalized to total protein from assorted types of white blood cells, each with varying cystine content.

METHODS
We present a new workflow for cystine determination using immunomagnetic granulocyte purification, and new reference ranges established from 47 patient and 27 obligate heterozygote samples assayed. Samples were collected in acid-citrate dextrose tubes and their stability was proven to allow for overnight shipping before analysis. Cystine was quantified by LC-MS/MS.

RESULTS
The new method was reproducible (&lt;15% root mean square error) and specific, assaying purified granulocytes from blood samples that no longer required immediate preparation and therefore allowing for up to 30 h before processing. There was a nearly a 2-fold increase in the therapeutic target (1.9 nmol half-cystine/mg protein) range, established using distributions of patient, obligate heterozygote, and control samples. The 2.5–97.5 percentile ranges (−2 SD to +2 SD around mean) for these cohorts were 0.67–6.05 nmol/mg protein for patients, 0.33–1.35 nmol/mg protein for obligate heterozygotes, and 0.09–0.35 nmol/mg protein for controls.

CONCLUSIONS
The intracellular cystine determination method using immunopurified granulocytes followed by LC-MS/MS analysis improves the inherent variability of mixed leukocyte analysis and eliminates the need for immediate sample preparation following blood draw.
The evaluation of a school according to supervision reports within the process<p>Denetim raporlarına göre bir okulun süreç içerisinde değerlendirmesi
Authors: Yavuz Mustafa , Gülmez Deniz 
Published: March 2016

The purpose of the study is to evaluate the supervision reports written on the basis of primary school supervisors’ supervision in a primary school. The sample consists of supervision reports of a primary school which was a middle school before 1997 in Konya/Meram. A case study design was used. Data was obtained through document analysis of qualitative research techniques. Documents consist of reports written by primary supervisors between the years of 1965-2008. The data were analyzed by content analysis. Content analysis was done according to phases that stated by Yildirim & Simsek (2006) as 1) Coding the data 2) Finding themes 3) Organizing the codes and themes 4) Commenting the findings. Reports that written by supervisors between 1965-2008 were coded by year. Coded reports split up themes using content analysis. According to the results, supervision reports divided into five themes: physical condition, education-instruction, account-belongings, school-environment relationship and school management. ÖzetAraştırmanın amacı, ilköğretim denetmenlerinin bir ilköğretim okulunda yaptıkları denetime dayalı olarak yazdıkları denetim raporlarının değerlendirilmesidir. Çalışma grubu Konya ili Meram ilçesinde yer alan 1997’den önce ortaokul olan bir ilköğretim okulunun denetim raporlarından oluşmaktadır. Veriler, nitel araştırma tekniklerinden doküman incelemesi yoluyla toplanmıştır. Doküman incelemesi, mevcut kayıt ya da belgelerin, veri kaynağı olarak sistemli incelenmesi şeklinde ifade edilmektedir. Dokümanlar, Konya ilköğretim denetmenlerinin 1965–2008 yılları arasında yazdıkları raporlardan oluşmaktadır. Veriler, içerik analizi yöntemi ile analiz edilmiştir. Araştırmada içerik analizi için Yıldırım ve Şimşek (2006) tarafından belirtilen (1) verilerin kodlanması, (2) temaların bulunması, (3) kodların ve temaların düzenlenmesi ve (4) bulguların yorumlanması aşamalarına göre yapılmıştır. Denetmenler tarafından 1965-2008 yılları arasında yazılan raporlar yazıldığı yıla göre kodlanmıştır. Kodlanan raporlar içerik analizi ile temalara ayrılmıştır. Analiz sonuçlarına göre, denetim raporları fiziki durum, eğitim-öğretim, hesap-ayniyat, okul-çevre ilişkileri ve okul yönetimi olmak üzere beş temaya ayrılmıştır.
Relationship between stigma levels of teachers and teacher’s organizational justice perceptions<p>Öğretmenlerin damgalama düzeyleri ile örgütsel adalet algıları arasındaki ilişki
Authors: Önk Murat , Cemaloğlu Necati 
Published: March 2016

The purpose of this study was to determine the relationship between stigma levels of teachers and teachers’ organizational justice perceptions. Relational survey model was used in research. Teachers’ opinions on stigma level and organizational justice were reviewed. A total of 204 public school teachers (elemantary and high) employed in Ankara participated in the study. The "Stigma Scale” developed by Yaman and Güngör (2013) and "Organizational Justice Scale”developed by Hoy and Tarter (2004) were used to gather data. The data was analyzed by using Pearson Product-Moment Correlation Coefficient, Descriptive Statistics (frequency, percentage, arithmetic average, standard deviation), and multiple regression analysis techniques. Results indicated that teachers’ stigma scores were high in prejudice and psychological health and the participants had positive perceptions about organizational justice at public schools. There was meaningful negative relationship between stigma levels of teachers and organizational justice perceptions of teachers. Consequently, stigma level is one of the factors that affect organizational justice negatively in schools. ÖzetBu araştırmanın amacı, Ankara Büyükşehir Belediyesi sınırları içerisinde bulunan merkez ilçelerdeki resmi ilköğretim ve ortaöğretim kurumlarında görev yapan öğretmenlerin görüşlerine göre, öğretmenlerin damgalama düzeyleri ile örgütsel adalet algıları arasındaki ilişkiyi saptamaktır. Araştırma, ilişkisel tarama modelindedir. Araştırma modelinde, biri bağımsız/dışsal (öğretmenlerin damgalama düzeyi), biri bağımlı/içsel (öğretmenlerin örgütsel adalet algıları) olmak üzere iki değişken bulunmaktadır. Araştırmanın örneklemini, Ankara Büyükşehir Belediyesi sınırları içerisinde bulunan merkez ilçelerdeki resmi ilköğretim ve ortaöğretim kurumlarında görev yapan toplam 204 öğretmen oluşturmaktadır. Araştırmada veri toplama aracı olarak, Yaman ve Güngör (2013) tarafından geliştirilen “Damgalama Ölçeği” ile Hoy ve Tarter (2004) tarafından oluşturulan “Örgütsel Adalet Ölçeği” kullanılmıştır. Verilerin analizinde betimsel istatistikler, Pearson Momentler Çarpımı Korelasyon Katsayısı ve Çoklu Regresyon Analizi tekniklerinden yararlanılmıştır. Araştırmanın sonuçlarına göre; öğretmenlerin damgalama eğilimlerinin önyargı ve psikolojik sağlık alt boyutlarında yüksek olduğu, katılımcıların okullarındaki örgütsel adalet algısını olumlu olarak belirttiği, öğretmenlerin damgalama düzeyleri ile örgütsel adalet algıları arasında anlamlı ve negatif bir ilişki olduğu, öğretmenlerin örgütsel adalet algısının en önemli yordayıcısının ön yargı olduğu saptanmıştır.
KAJIAN PERKEMBANGAN FISIK DAN TIPOLOGI KAWASAN PERMUKIMAN DI PUSAT PERTUMBUHAN KECAMATAN TEMBALANG, KOTA SEMARANG
Authors: Suryani Tia Adelia , Rahdriawan Mardwi 
Published: March 2016

<p><em>Perkembangan kota terjadi akibat munculnya pusat pertumbuhan baru. Kecamatan Tembalang menjadi pusat pertumbuhan baru </em><em>karena </em><em>adanya kebijakan pemerintah yang menjadikan Kecamatan Tembalang sebagai kawasan pendidikan, Kecamatan Tembalang yang merupakan lokasi kawasan pendidikan merupakan salah satu pusat pertumbuhan kota Semarang menjadi pendorong berkembangnya segala akiti</em><em>v</em><em>itas yang berimplikasi pada terjadinya perubahan pemanfaatan lahan. Bertambah luasnya kawasan permukiman dan berubahnya kondisi permukiman </em><em>kemudian mengindikasikan adanya perbedaan jumlah dan kondisi</em><em> sarana prasarana </em><em>serta</em><em> kualitas permukiman. Penelitian ini bertujuan untuk mengkaji Perkembangan Fisik dan Tipologi Kawasan Permukiman di Pusat Pertumbuhan Kecamatan Tembalang, Kota Semarang, menggunakan metode deskriptif kuantitatif dengan analisis spasial yang menggunakan Data Citra</em><em>.</em><em> Hasil penelitian menunjukkan</em><em> t</em><em>erjadi perkembangan fisik di kawasan permukiman selama kurun waktu 10 tahun (2005-2015). Tipologi kawasan permukiman di Kecamatan Tembalang, mencerminkan suatu permukiman perkotaan yang telah dilengkapi dengan sarana prasarana, namun yang berada dalam kondisi baik hanya ditemukan di Kelurahan Sendangmulyo. Kualitas lingkungan Kawasan Permukiman sebagian besar termasuk dalam kriteria cukup layak ditemukan di Kelurahan Rowosari, Kelurahan Meteseh, Kelurahan Kramas, Kelurahan Tembalang, Kelurahan Bulusan, Kelurahan Mangunharjo, Kelurahan Sambiroto, Kelurahan Jangli, Kelurahan Tandang, Kelurahan Kedungmundu, dan Kelurahan Sendangguwo. Hal ini terjad</em><em>i</em><em> karena kondisi pohon pelindung dan pola tata letak bangunannya buruk. Rekomendasi yang dapat disampaikan berdasarkan hasil penelitian yaitu perlu dilakukan perbaikan prasarana lingkungan dan perbaikan Pola tata letak bangunan serta pengaturan pohon pelindung di Kawasan Permukiman Kecamatan Tembalang.</em></p>
TINJAUAN KRITIS PROYEK RENCANA TINDAK PENATAAN LINGKUNGAN PERMUKIMAN TRADISIONAL / BERSEJARAH KABUPATEN MAGELANG
Authors: Cintiyadewi Mariana Jayanti 
Published: March 2016

<p>Dusun Mantran Wetan merupakan salah satu dusun yang masuk dalam rangkaian Festival 5 Gunung dan sampai saat ini masih mempertahankan tradisinya baik dari segi kebudayaan maupun lingkungan binaannya (permukiman). Hal ini dibuktikan dengan masih banyaknya penduduk yang mempertahankan keaslian wujud permukimannya berupa bangunan Limasan. Dewasa ini banyak terjadi fenomena pembangunan yang lebih mengedepankan nilai ekonomi dan mengakibatkan pergeseran nilai-nilai sosial dan budaya. Pergeseran nilai tersebut menimbulkan dampak perubahan pada tatanan lingkungan binaan seperti terjadinya perubahan pada wajah lingkungan permukiman. Untuk menangani permasalahan tersebut pemerintah melakukan kegiatan penanganan yaitu revitalisasi lingkungan permukiman tradisional sebagai upaya mencapai keberlanjutan (sustainability). Kegiatan ini bertujuan untuk meningkatkan kualitas lingkungan permukiman yang memiliki khasanah yang berpotensi untuk dikembangkan sebagai unsur penggerak ekonomi masyarakat, wisata, dan atau tempat tinggal komunitas. Penanganan permukiman tradisional ini juga diharapkan menjadi asset wisata dan pelestarian permukiman tradisional. Jurnal ini akan mengidentifikasi substansi penanganan permukiman dari aspek fisik, dan non fisik (sosial, ekonomi, budaya) dengan menggunakan metode deskriptif dari data primer maupun sekunder yang telah diperoleh. </p>
KAJIAN EKONOMIS PENGGUNAAN JALAN SIMPANG TIDAK SEBIDANG DI KORIDOR JATINGALEH KOTA SEMARANG
Authors: Kunmaryati Endah 
Published: March 2016

<p>Koridor Jatingaleh merupakan salah satu jalan yang menghubungkan pusat Kota Semarang dengan Kota Semarang bagian selatan yang memiliki pergerakan yang sangatn tinggi. Koridor Jatingaleh merupakan salah satu titik macet di Kota Semarang. Terjadinya kemacetan di kawasan ini disebabkan karena volume kendaraan yang melampaui kapasitas jalan yang ada. Oleh karena itu perlu dilakukan pembangunan jalan simpang tidak sebidang (<em>flyover atau underpass</em>) guna mengatasi kemacetan di Koridor Jatingaleh. Selanjutnya perlu dikaji pula, alternatif jalan simpang tidak sebidang mana yang lebih sesuai untuk dibangun? <em>Flyover</em> atau <em>Underpass</em>? Oleh karena itu, penelitian ini bertujuan untuk mengkaji alternatif jalan simpang tidak sebidang yang sesuai dari aspek ekonomi guna menangani kemacetan di koridor Jatingaleh. Penelitian ini menggunakan pendekatan kuantitatif. Hasil penelitian yang didapatkan adalah pembangunan <em>underpass</em> lebih hemat dibandingkan dengan pembangunan <em>flyover</em>. Kondisi tersebut ditinjau dari perhitungan nilai waktu, BOK dan biaya pemeliharaan dan juga pengaruhnya bagi struktur ruang kawasan sekitarnya. Dengan demikian, dapat diketahui jalan simpang tidak sebidang yang sesuai dari aspek ekonomi di koridor Jatingaleh adalah<em> underpass</em>. </p>
ANALISIS KINERJA PENGELOLAAN PASAR TRADISIONAL DI KOTA SALATIGA
Authors: Prasetyo Sabdo Budi , Wahyono Hadi 
Published: March 2016

<p>Kontribusi pendapatan retribusi pasar terhadap penerimaan retribusi daerah Kota Salatiga cenderung menurun dari 26,35% pada tahun 2009 menjadi 17,70% pada tahun 2013. Beberapa bangunan pasar tradisional juga masih belum berfungsi optimal. Penelitian ini bertujuan untuk mengetahui kinerja pengelolaan pasar tradisional di Kota Salatiga dan menganalisis faktor penyebabnya, dengan menggunakan pendekatan deskriptif kuantitatif. Pengukuran kinerja menggunakan konsep Balanced Scorecard yaitu berdasarkan aspek finansial dan non finansial. Analisis tingkat kepentingan dan kinerja digunakan untuk mengetahui kesenjangan, tingkat kepentingan, kinerja dan kapasitas organisasi pengelola pasar tradisional di Kota Salatiga. Hasil akhir penelitian menunjukkan skor kinerja perspektif pertumbuhan dan pembelajaran sebesar 70,6%, proses internal 60,87%, finansial/keuangan 62,06%, kepuasan pelanggan internal 65,02% dan kepuasan pelanggan eksternal 55,58% diperoleh rata-rata sebesar 62,826% sehingga dapat disimpulkan kinerja pengelolaan pasar tradisional di Kota Salatiga masih kurang baik. Sebanyak 61% pedagang dan 90% pengunjung menyatakan belum nyaman dan belum puas dengan kondisi pasar tradisional. Faktor penyebab masih rendahnya kinerja pengelolaan pasar adalah belum terbentuk budaya kerja yang mampu meningkatkan motivasi pegawai, minimnya ketersediaan dan pemeliharaan sarana prasarana kerja, mekanisme kerja yang belum jelas dan belum dipahami seluruh pegawai, minimya motivasi dari pimpinan kepada pegawai pengelola pasar dan keuangan belum efisien.. </p>