bluuebunny/crossref_metadata_2025_split huggingface dataset:
Own Experience From The Use Of A Substitute Of An Allogeneic Acellular Dermal Matrix Revitalized With In Vitro Cultured Skin Cells In Clinical Practice
Authors: Łabuś Wojciech , Kawecki Marek , Glik Justyna , Maj Mariusz , Kitala Diana , Misiuga Marcelina , Klama-Baryła Agnieszka , Kraut Małgorzata , Nowak Mariusz 
Published: January 2016

AbstractAs a result of the removal of cells from human allogeneic dermis, a collagen scaffold is obtained, which can be populated de novo with autologous/allogeneic skin cells and transplanted onto the area of skin loss. The optimal method for production of acellular dermal matrices (ADM) has been selected. Three female patients (a mean age of 54 years) were subjected to the transplantation of either autologous or allogeneic keratinocytes and fibroblasts into the holes of acellular dermal matrix (ADM) mesh graft. The method for burn wound treatment based on the use of a viable dermal-epidermal skin substitute (based on ADM and in vitro cultured fibroblasts and keratinocytes) may be the optimal method of burn treatment.
A Rare Presentation Of An Ectopic Breast Tissue In Axilla
Authors: Vidyasagar Radhika , Sudarshan  , Singh R. N , Shivaram  
Published: January 2016

AbstractAccessory breast tissue is rare accounting to less than 1% cases seen in females. It is usually bilateral. We report a case of 24-year-old woman with a lump in the left axilla in view of its rarity and made a differential diagnosis of fibroadenoma, which following the investigations and histopathological report was confirmed as revealed fibroadenoma in the axilla. It should also be considered as a differential diagnosis for all axillary swellings.
Assessment of iron in uterine and testicular tissues and hair of free-ranging and household cats
Authors: Rzymski P. , Niedzielski P. , Dąbrowski P. 
Published: January 2016

AbstractIron (Fe) represents a highly essential element for various biological processes. In spite of this very little is known as regards its status in mammalian reproductive tissues and factors that may potentially influence it. At the same time, there is an ongoing debate as to whether analyses of the Fe content in hair can provide reliable information on its tissue burden. Therefore, the aim of the present study was to investigate the content of Fe in the testicular and uterine tissues, and hair of the domestic cat (Felis catus) and how this content relates to an animal’s age, weight, physical activity, inhabited environment and diet. The median Fe content in the feline reproductive tissues amounted to 50.8 ppm and in hair to 180.2 ppm. As found, free-ranging cats were characterized by a significantly higher Fe content in reproductive tissues, particularly in the uterus. Age, weight and physical activity had no effect on determined Fe levels. The type of commercial diet (wet, dry or combined) given to household cats also had no influence upon Fe status in hair and tissue although males fed exclusively on dry food had a lower Fetestis:Fehairratio. Hair Fe level was positively correlated with that found in the reproductive tissues (Rs=0.30). This study extends the body of information on Fe distribution in felines, demonstrates the difference between free-ranging and household cats and provides evidence that Fe hair status may, at least partially, reflect the status of this element in the feline reproductive system.
Fungal rhinitis in dogs
Authors: Ostrzeszewicz M. , Sapierzyński R. 
Published: January 2016

Abstract
Fungal rhinitis and sinusitis in dogs are quite common reasons of chronic nasal discharge and rhinoscopy in such cases is commonly suggested. Forty three dogs were examined using rhinoscopy because of the presence of chronic airway symptoms. Clinical examination, routine hematology and serum biochemistry profiles, nasal and frontal sinus radiographs were made in all animals. Additionally, computed tomography in one dog was performed. Samples for histopathology were taken from 9 patients during rhinoscopy, additionally, from 8 of these patients samples for cytopathology were collected by blind nasal swab technique. In 9 of 43 dogs (20,5%), all males aged 1 to 13 years, examinations led to a diagnosis of fungal rhinitis. In 2 cases a diagnosis of fungal rhinitis was obtained based solely on cytopathology, while in 7 cases – mycosis of nasal mucosa was confirmed by histopathology. The present study revealed that cytopathological examination of nasal swabs has a low diagnostic value in the case of nasal infections in dogs. Although, in some dogs cytopathology, together with other widely available diagnostic techniques was sufficient to reliably diagnose fungal rhinitis, histopathology of samples collected during rhinoscopy is still the gold standard in such cases.
The effect of yeast Yarrowia lipolytica on the antioxidant indices and macro-and microelements in blood plasma of turkey hens
Authors: Merska M. , Czech A. , Ognik K. 
Published: January 2016

Abstract
The aim of the study was to assess the effect of different amounts of Yarrowia lipolytica yeast on the redox response and content of macro- and microelements in the blood plasma of turkey hens. The experiment was carried out on 240 turkey hens aged from 1 to 16 weeks. The birds were randomly assigned to 3 experimental groups of 80 birds each. Group I served as a control (K) and did not receive any experimental compounds. The turkey hens from the experimental groups (YL3 and YL6) were administered dried Yarrowia lipolytica yeast in their feed mixtures in the amount of 3% (YL3) or 6% (YL6).
Yarrowia lipolytica yeast in the feed mixtures for the turkey hens did not induce oxidation reactions in the organism of the birds. However, an increase in catalase activity and a reduction in the level of LOOH, MDA and vitamin C were observed in the blood plasma of the turkey hens whose diet was supplemented with YL yeast. In the case of other indices, such as superoxide dismutase activity and total antioxidant potential (FRAP), the additive caused no significant changes. Administering Yarrowia lipolytica yeast to turkey hens may stimulate the enzymatic response of the antioxidant system (e.g. increasing catalase activity), mainly by increasing the concentration of iron in the plasma.
Effects of dietary supplementation with sage (Salvia officinalis L.) essential oil on antioxidant status and duodenal wall integrity of laying strain growers
Authors: Placha I. , Ryzner M. , Cobanova K. , Faixova Z. , Faix S. 
Published: January 2016

Abstract
The objective of this study was to compare the influence of four different concentrations of Salvia officinalis essential oil (EO) on animal health. A total of 50 laying strain chicks were randomly divided at the day of hatching into five dietary-treatment groups. Control group was given the basal diet (BD), the other four experimental groups contained BD supplemented with 0.1, 0.25, 0.5, 1.0 g S. officinalis EO/kg diet, respectively. 0.1 g/kg EO increased glutathion peroxidase activity (GPx) in duodenal mucosa, liver and kidney, phagocytic activity in blood (PA), transepithelial electrical resistance (TEER) in duodenal tissue and decreased malondialdehyde (MDA) concentration in plasma and liver. 0.25 g/kg EO increased GPx in liver, total antioxidant status (TAS) in plasma, PA in blood and TEER in duodenal tissue. Our results demonstrate that lower concentrations of EO improve animals’ health status, and that it is necessary keep in mind the selection of sufficient concentration of EO used as animal feed additive.
Detection of Pentatrichomonas hominis in dogs using real-time PCR
Authors: Michalczyk M. , Sokół R. , Socha P. 
Published: January 2016

Abstract
Trichomonadidae family is a protozoan occurring in different animal species. It inhabits the gastrointestinal and urinary tracts. P. hominis is rarely found in faecal samples of dogs, and its identification and differentiation from other trichomonads by light microscopy are difficult. Methods of molecular biology are the most effective in this case, because they confirm the presence of the specific species in animal organisms, irrespective of the protozoan form. The aim of this study was to find P. hominis in selected dog kennels in North-Eastern Poland. Forty-one faecal samples of dogs from 7 dog kennels were examined. The occurrence of P. hominis in 5 faecal samples of dogs with no symptoms of diarrhoea was the first one to be confirmed in Poland.
Etiology and pathology of epidemic outbreaks of avian influenza H5N1 infection in Egyptian chicken farms
Authors: Ali A. , Elmowalid G. , Abdel-Glil M. , Sharafeldin T. , Abdallah F. , Mansour S. , Nagy A. , Ahmed B. , Abdelmoneim M. 
Published: January 2016

AbstractEpidemic outbreaks of avian influenza (AI) virus H5N1 have been frequently reported in Egypt during the last nine years. Here we investigate the involvement of AI H5N1 in outbreaks of acute respiratory disease that occurred in several commercial chicken farms in Egypt in 2011, and we describe to the pathology caused by the virus in the course of the outbreak.Twenty-one chicken farms with history of acute respiratory symptoms and high mortalities were screened for AI H5N1. Virus identification was based on hemagglutination inhibition test, and PCR detection and sequencing of the hemagglutinin and neuraminidase genes. Virus distribution was determined by immunohistochemical staining of AI antigens in organs of infected birds. Standard H&E staining was performed for histological examination of affected organs.Eighty-one % of the examined birds, representing 100% of the screened farms, were positive for AI H5N1 virus. Phylogenetic analysis of the hemagglutinin and neuraminidase genes of the isolated virus reveals its affiliation to clade 2.2.1. Viral antigens were localized in the endothelial cells of the heart, liver, lungs and skin, where pathological lesions including congestion, hemorrhages, multifocal inflammation and necrosis were concurrently observed. According to the pattern of the viral antigen and lesion distribution in the visceral organs, we suggest cardiovascular and circulatory failures as the probable cause of death during these outbreaks. In conclusion, the present study further confirms the epidemic status of AI H5N1 virus in Egypt and reveals the highly pathogenic nature of the local isolates.
Biochemical and immunological responses of young turkeys to vaccination against Ornithobacterium rhinotraheale and different levels of dietary methionine
Authors: Kubińska M. , Tykałowski B. , Koncicki A. , Jankowski J. 
Published: January 2016

AbstractThe objective of this study was to verify the hypothesis that increasing levels of dietary methionine can stimulate the mechanisms of cell-mediated and humoral immunity in young turkeys. The blood and organs involved in cell-mediated and humoral immune responses were analyzed in 8-week-old turkeys that had been vaccinated againstOrnithobacterium rhinotraheale(ORT) infection (on days 17 and 48). The birds were fed diets with a low (LM), medium (MM) and high (HM) methionine content (0.45 and 0.40%, 0.60 and 0.51%, 0.71 and 0.57% in weeks 1 – 4 and 5 – 8, respectively).Dietary methionine supplementation led to a significant increase in body weights of turkeys at 56 days of age, from 3532 g in group LM to 3720 g in group MM and 3760 g in group HM (p=0.001). A significant increase in vaccine-induced antibody titers against ORT was noted in group HM relative to group LM (p=0.006). Increasing levels of methionine had no significant effect on total serum IgG nor IgM levels and most serum biochemical parameters, TP, ALB, GLOB, GLU, AST, ALP, P and Ca. In comparison with group LM, group HM turkeys were characterized by a lower percentage of IgM+B cell subpopulation in the blood and bursa of Fabricius.The percentages of CD4+and CD8+T cell subpopulations in the bursa of Fabricius in group HM were significantly different from those found in groups LM and MM.The highest percentages of CD4+T cells and CD8+T cells in the spleen were observed in groups LM (p<0.001) and HM (p=0.04), respectively. The differences were statistically significant relative to the remaining groups. Turkeys of group LM were characterized by a lower CD4+T cell percentage in the thymus (p<0.001) and a lower CD8+T cell percentage in the cecal tonsils (CTs) (p<0.01). Vaccination against ORT resulted in a significant increase in the percentage of CD4+/CD8+T cell subpopulation and a decrease in the percentage of CD8+T cell subset in the spleen.
Application of real-time PCR for evaluation of distribution of equine herpesvirus type 1 in tissues of aborted fetuses
Authors: Stasiak K. , Rola J. , Zmudzinski J.F. 
Published: January 2016

AbstractA highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0×100of viral DNA copies and the obtained standard curve exhibited a linear range from 100to 107molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real-time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ctvalue: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).
Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla
Authors: Szalak R. , Matysek M. , Mozel S. , Arciszewski M.B. 
Published: January 2016

Abstract
Calretinin (CR) as a buffer and sensor protein plays an important role in regulatory processes of Ca2+ and anty-apoptotic cellular protection. In the present study, immunohistochemical peroxidase-antiperoxidase (PAP) method was used in order to determine the numbers, morphology, morphometry and distribution pattern of CR in neurons of the chinchilla’s claustrum (Cl) and endopiriform nucleus (EN). In Cl and EN the presence of several classes of neurons with different immunoreactivity to CR was found. In Cl, CR-immunoreactive (IR) neurons were predominantly found in close vicinity to insular border while CR-IR neurons were evenly scattered throughout EN. In general, immunoreaction to CR was observed in neuronal cytoplasm, nucleus and in fibres-like nerve extensions. Statistical analysis showed the differences between average large diameter as well as cross-sectional area of CR-IR neurons present in Cl and EN. It is suggested, that CR acting as a calcium binding protein may play a role in neuronal network. Further co-localization studies are necessary to fully elucidate neurophysiology and neuropathology of the chinchilla’s Cl and EN neurons.
Clinical observation of a professional tattooing procedure and evolutionary study of the skin damage provoked
Authors: Pedrol Joan González , Trull Josep Lloreta , Sánchez Mercè Campoy , Romero Julián López , Martínez-Carpio Pedro A. , Trelles Mario A. 
Published: January 2016

Abstract

Objective:
A clinical observation of a professional tattooing procedure and the skin damage caused by the tattooing technique and the healing process of the lesions.

Materials and methods:
Three tattoos were performed using black, red and white ink, together with a fourth “tattoo” with the same characteristics and an identical procedure, but using no ink. The evolution of the lesions was observed over a period of 6 months using photographs and biopsies.

Results:
The tattoos evolved with no complications. At 72 h, full destruction of the epidermis and de-structuring of the papillary dermis were confirmed. At 3 months, an almost ad integrum restoration of the skin structure was observed with a settling of the inks on the reticular dermis. With regard to the inkless tattoo, a wound with self-limiting capillary bleeding was observed which gradually healed, leaving only a sclerotic and hyperpigmented residual lesion by month 6.

Conclusion:
An awareness of the tattooing procedure would lead to a better understanding of potential complications, which might arise. This study contains the description and evolution of the lesions and provides the first images of skin damage caused by contemporary professional tattooing techniques, without the masking factor of the ink.
Prevalent Parasitosis in Beef and Dairy Cattle Farms in Vidzeme Region
Authors: Keidāne Dace , Krūklīte Anna , Derbakova Alīna 
Published: January 2016

Abstract
The aim of the study was to investigate the beef and dairy cow parasitosis epizootic situation in Vidzeme region. Research was done throughout Vidzeme territory during the period of the years 2013-2014. The total number of animals examined was: 273 dairy and 90 young beef cattle aged from 6 months to two years and 248 dairy and 29 beef cows older than two years. For the diagnosis of helminthes standardized ovoscopic and larvoscopic methods were used. For the diagnosis of protozoa flotation and modified Ziehl-Neelsen methods were used. The main species in the samples were Cryptosporidium spp., Eimeria spp. and Strongylus spp. In the young dairy and beef cattle aged from 6 months to two years and cattle older than two years Cryptosporidium spp. invasion accordingly was 32.6% and 19% (dairy cattle) and 62.2% and 65.5% (beef cattle); the invasion of Eimeria spp. 30% and 7.3% (dairy cattle) and 55.6% and 10.3% (beef cattle); and the invasion of Strongylus spp. was 17.6% and 13.7% (dairy cattle) and 43.3% and 27.6% (beef cattle). Both dairy and beef cattle were infected with Moniezia spp., Paramphistomum spp., Strongyloides spp. Dairy cows aged from 6 months to two years had Trichuris spp., Dictyocaulus spp. and Neoscaris spp. invasion.
Leachates of Thermally Modified Pine (Pinus sylvestris L.) Wood
Authors: Meija-Feldmane Anete 
Published: January 2016

Abstract
During the last decades, thermally modified wood has become an object of interest among the wood scientists as an environmentally friendly material, because nowadays environmental aspects of materials have become more and more significant. Leaching is one of the processes that occurs in outdoor use. The aim of this study was to evaluate concentration of potentially hazardous substances in leachates of thermally modified pine wood. Scots pine (Pinus sylvestris L.) wood was thermally modified using Wood Treatment Technology (WTT) company device at 170 °C for 1 hour (TMP170/1) and at 160 °C for 3 hours (TMP160/3) and the mass loss was calculated. Material preparation and leaching procedure was made according to standard LVS EN 84:2000. In obtained leachates, the content of sugars, acetic acid, furfural and tannic acid were determined. Results showed that the total wood mass loss was 7.1 ± 1.4% (n=20) for TMP170/1 and 4.0 ± 1.6% (n=20) for TMP160/3. The initial leaching velocity differs between both modes and is higher for TMP160/3. The velocity decreases exponentially with immersion time and reaches plateau after 7th (5 days) immersion, but leaching still continues after the 9th immersion (14 days). The main components in leachates were tannic acid and pentoses. Among all studied compounds furfural is the hardest leachable one. Thermally modified wood treated at TMP170/1 is more environmentally friendly due to less water leachable substances. It is worth looking forward by investigating volatile organic compounds emissions in the air as it also could give high impact on human health.
Congruent Representation: Election Cycle in Poland 2009-2011
Authors: Wojtasik Waldemar 
Published: January 2016

Abstract
The article presents the results of research on the congruence of the political representation formed in elections held in the years 2009-2011 in Poland. The election cycle included the European Parliamentary elections in 2009, the Polish presidential election, elections to local government in 2010, and the parliamentary elections in 2011. The median citizen, median voter, and their positions on the left-right scale were used as tools for examining congruence. Studies have proven that in Poland, the median citizen and the median voter are positioned on the right side of the left-right scale. The legislature and executive authorities chosen in the elections are located left of the median citizen and the median voter. Studies have not demonstrated the existence of any impact of the electoral system on the positioning of the median citizen and the median voter.
Russia’s Containment and Engagement Strategies in the U.S. Foreign Policy during the Presidency of G. W. Bush
Authors: Jakstaite Gerda 
Published: January 2016

Abstract
Traditionally, Containment and Engagement strategies are considered to be the part of the United States foreign policy during the Cold War. However, recent developments in international relations indicated that these strategies are still relevant to the contemporary foreign policy of the U.S., particularly in the U.S.-Russian relations. Contradictory presidency of George W. Bush has raised a question which of the mentioned foreign policy strategies was dominating in the U.S.-Russian relations. On the one hand, U.S. officials had declared that partnership with Russia was being pursued. On the other hand, the administration of G.W. Bush favored the expansion of NATO and did not surrender the initiative of missile defense shield. This paper intends to assess which foreign policy strategy (Containment or Engagement) dominated in U.S.-Russian relations during the presidency of G.W. Bush and to analyse reasons of such domination and the ways these strategies were implemented. The results of the research indicate that G. W. Bush administration implemented different foreign policy towards Russia on the declared and practical foreign policy levels. If on the official U.S. foreign policy level Russia’s engagement strategy dominated, in the U.S. foreign policy practice, particularly influenced by the foreign policy of Russia, and to a lesser extent by the events in the international arena, the dominant foreign policy strategy towards Russia was Russia’s containment strategy.
Epidemiology of musculoskeletal injury in the California film and motion picture industry
Authors: Kusnezov Nicholas A. , Yazdanshenas Hamed , Garcia Eddie , Shamie Arya N. 
Published: January 2016

AbstractMusculoskeletal injury exerts a significant burden on US industry. The purpose of this study was to investigate the frequency and characteristics of musculoskeletal injuries in the California (CA) film and motion picture (FMP) industry which may result in unforeseen morbidity and mortality.We reviewed the workers’ compensation (WC) claims database of the Workers’ Compensation Insurance Rating Bureau of California (WCIRB) and employment statistics through the US Bureau of Labor Statistics (BLS). We analyzed the frequency, type, body part affected, and cause of musculoskeletal injuries.From 2003 to 2009, there were 3505 WC claims of which 94.4% were musculoskeletal. In the CA FMP industry, the most common injuries were strains (38.4%), sprains (12.2%), and fractures (11.7%). The most common sites of isolated injury were the knee (18.9%), lower back (15.0%), and ankle (8.6%). Isolated musculoskeletal spine injuries represented 19.3% of all injuries. The most common causes of injury were work-directed activity (36.0%) and falls (25.5%).We present the first report on the unique profile of musculoskeletal injury claims in the FMP industry. This data provides direction for improvement of workplace safety.