bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular dissection of nuptial gifts in divergent strains of Ostrinia moths
Authors: Al‐Wathiqui Nooria , Lewis Sara M. , Dopman Erik B. 
Published: September 2017

AbstractSeminal fluid proteins (SFPs) produced in the male accessory glands and ejaculatory duct are subject to strong sexual selection, often evolve rapidly and therefore may play a key role in reproductive isolation and species formation. However, little is known about reproductive proteins for species in which males transfer ejaculate to females using a spermatophore package. By combining RNA sequencing and proteomics, we characterize putative SFPs, identify proteins transferred in the male spermatophore and identify candidate genes contributing to a one‐way gametic incompatibility between Z and E strains of the European corn borer moth Ostrinia nubilalis. We find that the accessory glands and ejaculatory duct secrete over 200 highly expressed gene products, including peptidases, peptidase regulators and odourant‐binding proteins. A comparison between Ostrinia strains reveals that accessory gland and ejaculatory duct sequences with hormone degradation and peptidase activity are among the most extremely differentially expressed. However, most spermatophore peptides lack reproductive tissue bias or canonical secretory signal motifs and aproximately one‐quarter may be produced elsewhere before being sequestered by the male accessory glands during spermatophore production. In addition, most potential gene candidates for postmating reproductive isolation do not meet standard criteria for predicted SFPs and almost three‐quarters are novel, suggesting that both postmating sexual interactions and gametic isolation likely involve molecular products beyond traditionally recognized SFPs.
Lipopolysaccharide and cytokines modulate leukotriene (LT)B4 and LTC4 production by porcine endometrial endothelial cells
Authors: Czarzasta J , Meller K , Andronowska A , Jana B 
Published: September 2017

ContentsUterine inflammatory response is mediated by inflammatory mediators including eicosanoids and cytokines produced by immune and endometrial cells. Interactions between lipopolysaccharide (LPS) and cytokines, and leukotrienes (LTs) in endothelium, important for the host defence during the inflammation, are unknown. We studied the effect of LPS, tumour necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐4 and IL‐10 on 5‐lipooxygenase (5‐LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) mRNA and protein expression, LTB4 and LTC4 release from porcine endometrial endothelial cells, and cell viability. For 24 hr, cells were exposed to LPS (10 or 100 ng/ml of medium) and cytokines (each 1 or 10 ng/ml). 5‐LO mRNA/protein expression augmented after incubation with larger doses of LPS, TNF‐α, IL‐4 and IL‐10 and smaller dose of IL‐1β. Larger dose of TNF‐α, smaller doses of LPS and IL‐1β and both doses of IL‐10 increased LTAH mRNA/protein expression. LTAH protein content was up‐regulated by larger dose of LPS, but it was reduced in response to both doses of IL‐4. LTCS mRNA expression was elevated by larger doses of LPS, IL‐4 and IL‐10 or both doses of TNF‐α and IL‐1β. LTCS protein level increased after treatment with both doses of IL‐1β, IL‐4 and IL‐10, smaller dose of LPS and larger dose of TNF‐α. Both doses of LPS and larger doses of TNF‐α and IL‐10 increased LTB4 release. LPS, IL‐1β and IL‐10 at smaller doses, or TNF‐α and IL‐4 at larger doses stimulated LTC4 release. Smaller doses of TNF‐α and IL‐1β or both doses of IL‐4 enhanced the cell viability. This work provides new insight on the participation of LPS, TNF‐α, IL‐1β, IL‐4 and IL‐10 in LTB4 and LTC4 production/release from porcine endometrial endothelial cells, and the effect of above factors on these cells viability. The used cellular model gives the possibility to further establish the interactions between inflammatory mediators.
Prostate vascular flow: The effect of the ejaculation on the power doppler ultrasonographic examination
Authors: Alonge S , Melandri M , Fanciullo L , Lacalandra GM , Aiudi G 
Published: September 2017

ContentsPower Doppler sonography (PD) can accurately depict tissue perfusion, recognize slow flows, and is relatively angle independent. The monitoring of local blood flow by Doppler ultrasonography is helpful in differentiating prostatic physio‐pathological conditions, but the recognizing of physiological variables that could affect it is crucial to apply this technique in clinical practice. This study aimed to evaluate if ejaculation affects blood flow to the prostate and to state how long this effect lasts. Serial PD examinations of prostate were performed in 18 dogs (1–5 years, 6–40 kg) immediately before (T0) and after (T1) the ejaculation, and repeated 6 (T2), 18 (T3) and 24 (T4) hours later. For each examination, two representative PD images were chosen and ranked by two independent observers according to the following scoring system: 0 = mild subcapsular (S) vascularization without clear evidence of parenchymal (P) vascularization; 1 = moderate P and S vascularization; 2 = severe S and moderate P vascularization; 3 = severe P and moderate S vascularization; 4 = severe P and S vascularization. Interobserver agreement was assessed using Kappa of Cohen. Ranked data, grouped according to time, were compared by ANOVA and Tukey HSD test (p < .05). Variations in the vascular flow pattern at different times were observed for all dogs. The statistical analysis evidenced a significant difference for T0 vs T1 and vs T2 and vs T3 (p < .01), with no significant difference for T0 vs T4 (p > .05). Interobserver agreement was very good (Kappa of Cohen = 0.86). This study demonstrated a definite increase in vascular flow to the prostate after ejaculation. The present results suggest a minimum of 24 hr sexual rest before the PD examination of the gland. This result should be taken into account whenever Doppler sonography is used to evaluate potential hyperaemia in dogs suspected of having prostate abnormalities.
Health‐related quality of life in patients with lymphoedema – a cross‐sectional study
Authors: Klernäs Pia , Johnsson Aina , Horstmann Vibeke , Johansson Karin 
Published: September 2017

IntroductionLymphoedema may cause complex problems that can strongly influence patients' health‐related quality of life (HRQoL). The main purpose of this study was to investigate the impact of lymphoedema on HRQoL in patients with varying forms of lymphoedema.MethodsThe Lymphoedema Quality of Life Inventory (LyQLI), measuring three domains, physical, psychosocial and practical, and the Short Form 36 Health Survey Questionnaire (SF‐36), measuring eight health domains, were sent to 200 lymphoedema patients. Out of those who answered both questionnaires, 88 patients had lymphoedema secondary to cancer treatment and they additionally received the Functional Assessment of Cancer Therapy Scale‐General (FACT‐G). The relation between continuous variables and the three domains were analysed by Spearman's correlation coefficients, and Kruskal–Wallis test was used to analyse categorical variables.ResultsAltogether 129 patients completed the LyQLI and SF‐36 and 79 of them also completed FACT‐G. Twenty per cent had a high mean score (≥2.0) in at least one domain of the LyQLI, thus having a low HRQoL. Lower HRQoL was found in the practical domain of LyQLI in patients with lower limb lymphoedema compared to patient with lymphoedema in upper limb or head/neck (p = 0.002) and in patients working part‐time compared to patients working full‐time (p = 0.005). The impact on HRQoL tended to decrease with age, with a significant correlation in the psychosocial domain (rs = 0.194, p = 0.028). Compared with the general Swedish population, patients with lymphoedema scored significantly lower in general health (p = 0.006), vitality (p = 0.002) and social functioning (p = 0.025) assessed by the SF‐36. From a cancer‐specific view, HRQoL was similar to other Swedish studies using the FACT‐G.ConclusionsThis study indicates that about 20% of the patients with lymphoedema had major impact on their HRQoL. More effort and research is needed to identify, understand and support groups of patients with severe lymphoedema‐related problems.
Composition and Variation Analysis of the T Cell Receptor β‐Chain Complementarity Determining Region 3 Repertoire in Neonatal Sepsis
Authors: Sun J. , Sun B. , Gao Y. , He F. , Yang L. , Wang M. , Zhou W. 
Published: September 2017

AbstractT cell receptor (TCR) diversity is clearly related to protection from infection. However, the characteristics of TCR diversity in neonates are not clear. In this study, we investigated the TCR diversity of neonates with sepsis. Twenty neonates with severe sepsis and eight matched neonates without infection were enrolled in the study. For the neonates with sepsis, EDTA‐anticoagulated blood was collected on day 1 after the diagnosis of sepsis and on day 7 of treatment. For the neonates without infection, blood was collected one time. DNA was extracted from peripheral blood mononuclear cells. The complementarity determining region 3 (CDR3) gene was analysed by multiplex PCR and high‐throughput sequencing. The CDR3 types and lengths were similar in patients and healthy controls. There was a significant difference in VJ gene usage among the three groups. Compared to the healthy neonates, the neonates with sepsis had different VJ pairs and generated different clonotypes. Although the TCR β‐chain diversity was generally lower in the neonates with sepsis, there was no significant difference in TCR β‐chain diversity between the patients and the healthy controls. Our data showed the characteristics of the TCR repertoire in neonates with sepsis, which represents a potentially valuable data set. This result is useful for understanding neonatal susceptibility to infection.
Thinking for themselves? The effect of informant independence on children's endorsement of testimony from a consensus
Authors: Einav Shiri 
Published: September 2017

AbstractTestimony agreement across a number of people can be a reassuring sign of a claim's reliability. However, reliability may be undermined if informants do not respond independently. In this case, social consensus may be a result of indiscriminate copying or conformity and does not necessarily reflect shared knowledge or opinion. We examined children's emerging sensitivity to consensus independence by testing whether it affected their judgements in a social learning context. Children ages 5, 6, and 8–9 years (N = 92), and 20 adults for comparison received conflicting testimony about an unfamiliar country from two consensual groups of informants: An independent group who responded privately and a nonindependent group who had access to each other's answers. We found increasing levels of trust in independent consensus with age. Adults and 8–9‐year olds preferred to accept the claims of the independent consensus, whereas 5‐year olds favored the claims of the nonindependent consensus and 6‐year olds were mixed. Although previous work has shown that children trust a consensus over a lone dissenter as young as 2 years, the developmental shift in this study indicates that children's reasoning about the nature of consensus and what makes it reliable continues to develop throughout middle childhood.
Aeromagnetic signature of an exhumed double dome system in the SW Grenville Province (Canada)
Authors: Dufréchou Grégory 
Published: September 2017

AbstractAnalysis of aeromagnetic data in the Grenville Province reveals the presence of two regional‐scale unmapped structural domes (the Morin and Mékinac‐Taureau domes) with an oval‐shaped magnetic pattern bounded by regional‐scale shear zones and a geometry that is similar to that produced in crustal flow models under extension, which predict two upright domes of foliation (double dome) separated by a steep shear zone. The Mékinac‐Taureau dome, a metamorphic core complex, and the Morin dome may have been exhumed by channel flow. Exhumation occurred by a combination of thrust, normal‐sense and wrench shear zones. The preservation of paragneisses in the Morin dome suggests that it underwent a lesser degree of exhumation than did the Mékinac‐Taureau dome. This study shows how the integration of local field information with magnetic data in a regional tectonic setting can reveal and delineate concealed gneiss domes and highlights a role for strike‐slip tectonics in the creation of regional structures involving the exhumation of deep crust.
Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance
Authors: Zhao Na , Wang Gang , Das Atze T. , Berkhout Ben 
Published: September 2017

ABSTRACT
Many potent antiviral drugs have been developed against HIV-1, and their combined action is usually successful in achieving durable virus suppression in infected individuals. This success is based on two effects: additive or even synergistic virus inhibition and an increase in the genetic threshold for development of drug resistance. More recently, several genetic approaches have been developed to attack the HIV-1 genome in a gene therapy setting. We set out to test the combinatorial possibilities for a therapy based on the CRISPR-Cas9 and RNA interference (RNAi) mechanisms that attack the viral DNA and RNA, respectively. When two different sites in the HIV-1 genome were targeted, either with dual CRISPR-Cas9 antivirals or with a combination of CRISPR-Cas9 and RNAi antivirals, we observed additive inhibition, much like what was reported for antiviral drugs. However, when the same or overlapping viral sequence was attacked by the antivirals, rapid escape from a CRISPR-Cas9 antiviral, assisted by the error-prone nonhomologous end joining (NHEJ) DNA repair machinery, accelerated the development of cross-resistance to the other CRISPR-Cas9 or RNAi antiviral. Thus, genetic antiviral approaches can be combined, but overlap should be avoided.
ROC-king onwards: intraepithelial lymphocyte counts, distribution & role in coeliac disease mucosal interpretation
Authors: Rostami Kamran , Marsh Michael N , Johnson Matt W , Mohaghegh Hamid , Heal Calvin , Holmes Geoffrey , Ensari Arzu , Aldulaimi David , Bancel Brigitte , Bassotti Gabrio , Bateman Adrian , Becheanu Gabriel , Bozzola Anna , Carroccio Antonio , Catassi Carlo , Ciacci Carolina , Ciobanu Alexandra , Danciu Mihai , Derakhshan Mohammad H , Elli Luca , Ferrero Stefano , Fiorentino Michelangelo , Fiorino Marilena , Ganji Azita , Ghaffarzadehgan Kamran , Going James J , Ishaq Sauid , Mandolesi Alessandra , Mathews Sherly , Maxim Roxana , Mulder Chris J , Neefjes-Borst Andra , Robert Marie , Russo Ilaria , Rostami-Nejad Mohammad , Sidoni Angelo , Sotoudeh Masoud , Villanacci Vincenzo , Volta Umberto , Zali Mohammad R , Srivastava Amitabh 
Published: September 2017

ObjectivesCounting intraepithelial lymphocytes (IEL) is central to the histological diagnosis of coeliac disease (CD), but no definitive ‘normal’ IEL range has ever been published. In this multicentre study, receiver operating characteristic (ROC) curve analysis was used to determine the optimal cut-off between normal and CD (Marsh III lesion) duodenal mucosa, based on IEL counts on >400 mucosal biopsy specimens.DesignThe study was designed at the International Meeting on Digestive Pathology, Bucharest 2015. Investigators from 19 centres, eight countries of three continents, recruited 198 patients with Marsh III histology and 203 controls and used one agreed protocol to count IEL/100 enterocytes in well-oriented duodenal biopsies. Demographic and serological data were also collected.ResultsThe mean ages of CD and control groups were 45.5 (neonate to 82) and 38.3 (2–88) years. Mean IEL count was 54±18/100 enterocytes in CD and 13±8 in normal controls (p=0.0001). ROC analysis indicated an optimal cut-off point of 25 IEL/100 enterocytes, with 99% sensitivity, 92% specificity and 99.5% area under the curve. Other cut-offs between 20 and 40 IEL were less discriminatory. Additionally, there was a sufficiently high number of biopsies to explore IEL counts across the subclassification of the Marsh III lesion.ConclusionOur ROC curve analyses demonstrate that for Marsh III lesions, a cut-off of 25 IEL/100 enterocytes optimises discrimination between normal control and CD biopsies. No differences in IEL counts were found between Marsh III a, b and c lesions. There was an indication of a continuously graded dose–response by IEL to environmental (gluten) antigenic influence.
Polymer nanocomposite dielectric and electrical properties with quantum dots nanofiller
Authors: Ahmed R. M. , Morsi R. M. M. 
Published: September 2017

Nanocomposite films of different contents of CdSe/ZnS quantum dots nanoparticles embedded in hosting matrix of polyvinyl chloride (PVC) were prepared by simple solution casting method. Electrical and dielectric properties of nanocomposites films were investigated in the temperature range 323–393 (K) and at frequencies (50–2000) kHz. The frequency dependence of AC conductivity was following the universal power law. The values of the frequency exponent, s, revealed that the conduction mechanism at low temperature is considered by small polaron tunneling model, whereas at high temperature, it is related to CBH model. The activation energy values [Formula: see text] were depending on nanoparticle concentration as well as frequency. Also, X-ray diffraction (XRD) enabled approximately estimating the average particle size of the nanoparticles incorporated in PVC.
Mechanisms Linking Global 5-Day Waves to Tropical Convection
Authors: King Malcolm J. , Wheeler Matthew C. , Lane Todd P. 
Published: September 2017

Abstract
Reanalysis data and satellite-derived rainfall measurements are examined to determine possible mechanisms linking the “5 day” Rossby–Haurwitz wave to localized variations of tropical convection. The mechanisms in all regions rely on the modulation of zonal winds near the equator by the wave, but the nature of these mechanisms depends strongly on local topography and local climate. In the upper Amazon basin, the wave modulates the strength of prevailing easterlies and thus the upslope flow and associated convection on the eastern edge of the Andes. Similar modulation of upslope flow is involved off the Panamanian and Colombian Pacific coasts, but the deflection and confluence of low-level wind in the presence of the Andes and moisture transports across the Andes from the Amazon basin are also factors. Similar deflection and confluence of winds around and through the Maritime Continent lead to low-level divergence and convection anomalies over the eastern Indian Ocean. Anomalous moisture transports from the Congo basin to the eastern and northeastern Gulf of Guinea due to the wave affect atmospheric moisture over the Gulf of Guinea and thus convection in the region. Over oceanic convergence zones, modulations of the prevailing winds by the wave affect the overall wind magnitude, changing evaporation from the ocean surface and atmospheric moisture. Most of these mechanisms arise from the nonuniform nature of Earth’s surface and suggest that other external Rossby–Haurwitz waves may have similar interactions with convection.
Is Hospitalization Necessary after Ear Surgery? A National Survey and Retrospective Review of Postoperative Events
Authors: Jelicic Tanja , Savage Julian Richard , Aron Margaret 
Published: September 2017

ObjectiveFirst, to survey our national otolaryngology colleagues on their postoperative care habits (hospitalization vs day surgery) after elective middle ear surgery. Second, to evaluate the necessity of hospitalization and safety of day surgery after these procedures.MethodsA national survey regarding postoperative habits after elective middle ear surgery was launched. Then, the cases of all patients having undergone these surgical procedures at our center between 2010 and 2016 were reviewed. They were divided into 2 groups: hospitalization and day surgery. Postoperative events during hospitalization and rate of consultation/readmission for day surgery were recorded.ResultsHeterogeneity in postoperative habits for most elective otologic surgery exists among otolaryngologists. For tympanoplasty, however, day surgery was uniformly favored. At our institution, 88.6% of hospitalization patients had no complications during their stay. Complications noted for others were nausea (7.2%), bleeding (3.1%), hematoma (0.5%), and sensorineural hearing loss (0.5%). In the day surgery group, 3.0% consulted within 48 hours following their procedure, and the readmission rate was 1.3%. Nausea was the only cause for readmission, and stapes surgery accounted for 100% of readmissions.DiscussionMost elective middle ear surgery can be safely performed as day care. Hospitalization does not provide care that could not have been provided at home in the majority of cases. Overnight hospital stay may be considered for stapes surgery.Implications for PracticeDay surgery for elective middle ear surgery is sufficient for most cases. Transferring these cases to day care should lower costs to our health care system and increase bed availability.
Basal Plate Myometrial Fibers and Hypertensive Disorders of Pregnancy: A Case–Control Study
Authors: Wang Ann A , Ernst Linda M , Miller Emily S 
Published: September 2017

Introduction Basal plate myometrium (BPMYO), the pathological presence of myometrial fibers in the basal plate, is a common finding on pathological examination of the placenta, yet its clinical correlates are not well studied. As myometrial fibers are frequently located in proximity to poorly converted maternal spiral arteries, our objective was to determine whether BPMYO is associated with hypertensive disorders of pregnancy (HDP), a well-known clinical sequela of abnormal maternal artery remodeling. Methods This case–control study included women who delivered a live-born singleton gestation whose placentas were sent for pathological examination. Cases were women with HDP (gestational hypertension, preeclampsia, or HELLP syndrome) as defined by American College of Obstetricians and Gynecologists. Controls were women without HDP. Women with chronic hypertension were excluded. The primary outcome was the presence of BPMYO. Secondary outcomes included the pathologic stage of BPMYO and the incidence of pathologically defined accreta. Each outcome was compared between cases and controls in bivariable and multivariable analyses. Results Of the 306 women who met inclusion criteria, 230 (75%) had HDP. BPMYO was present in 99 (32%) of placentas. Compared to controls, cases were younger, had higher body mass index, and were more likely to have diabetes, be nulliparous, deliver preterm, and have had a prior cesarean. There were no differences in the incidence of BPMYO, stage of BPMYO, or incidence of pathologically defined accreta between cases and controls. These findings persisted after controlling for potential confounders. Conclusions Although BPMYO may be more common in the setting of abnormal placental vasculature, there is no significant association between BPMYO and HDP.
Changes in Alternative Splicing as Pharmacodynamic Markers for Sudemycin D6
Authors: Thurman Morgan , van Doorn Jacob , Danzer Barbara , Webb Thomas R , Stamm Stefan 
Published: September 2017

Objective: The aim of the study was to define pharmacodynamic markers for sudemycin D6, an experimental cancer drug that changes alternative splicing in human blood. Methods: Blood samples from 12 donors were incubated with sudemycin D6 for up to 24 hours, and at several time points total RNA from lymphocytes was prepared and the pre-messenger RNA (mRNA) splicing patterns were analyzed with reverse transcription-polymerase chain reaction. Results: Similar to immortalized cells, blood lymphocytes change alternative splicing due to sudemycin D6 treatment. However, lymphocytes in blood respond slower than immortalized cultured cells. Conclusions: Exon skipping in the DUSP11 and SRRM1 pre-mRNAs are pharmacodynamic markers for sudemycin D6 treatment and show effects beginning at 9 hours after treatment.