bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex observation of scalp fast (40–150 Hz) oscillations in West syndrome and related disorders with structural brain pathology
Authors: Kobayashi Katsuhiro , Endoh Fumika , Agari Takashi , Akiyama Tomoyuki , Akiyama Mari , Hayashi Yumiko , Shibata Takashi , Hanaoka Yoshiyuki , Oka Makio , Yoshinaga Harumi , Date Isao 
Published: January 2017

SummaryWe investigated the relationship between the scalp distribution of fast (40–150 Hz) oscillations (FOs) and epileptogenic lesions in West syndrome (WS) and related disorders. Subjects were 9 pediatric patients with surgically confirmed structural epileptogenic pathology (age at initial electroencephalogram [EEG] recording: mean 7.1 months, range 1–22 months). The diagnosis was WS in 7 patients, Ohtahara syndrome in 1, and a transitional state from Ohtahara syndrome to WS in the other. In the scalp EEG data of these patients, we conservatively detected FOs, and then examined the distribution of FOs. In five patients, the scalp distribution of FOs was consistent and concordant with the lateralization of cerebral pathology. In another patient, FOs were consistently dominant over the healthy cerebral hemisphere, and the EEG was relatively low in amplitude over the pathological atrophic hemisphere. In the remaining 3 patients, the dominance of FOs was inconsistent and, in 2 of these patients, the epileptogenic hemisphere was reduced in volume, which may result from atrophy or hypoplasia. The correspondence between the scalp distribution of FOs and the epileptogenic lesion should be studied, taking the type of lesion into account. The factors affecting scalp FOs remain to be elucidated.
Assessment of early life stage mahi-mahi windows of sensitivity during acute exposures to Deepwater Horizon crude oil
Authors: Mager Edward M. , Pasparakis Christina , Schlenker Lela S. , Yao Zongli , Bodinier Charlotte , Stieglitz John D. , Hoenig Ronald , Morris Jeffrey M. , Benetti Daniel D. , Grosell Martin 
Published: January 2017

Abstract

Windows of exposure to a weathered Deepwater Horizon oil sample (slick A) were examined for early life stage mahi-mahi (Coryphaena hippurus) to determine whether there are developmental periods of enhanced sensitivity during the course of a standard 96-h bioassay. Survival was assessed at 96 h following oil exposures ranging from 2 h to 96 h and targeting 3 general periods of development, namely the prehatch phase, the period surrounding hatch, and the posthatch phase. In addition, 3 different oil preparations were used: high- and low-energy water accommodated fractions of oil and very thin surface slicks of oil (∼1 μm). The latter 2 were used to distinguish between effects due to direct contact with the slick itself and the water underlying the slick. Considering the data from all 3 exposure regimes, it was determined that the period near or including hatch was likely the most sensitive. Furthermore, toxicity was not enhanced by direct contact with slick oil. These findings are environmentally relevant given that the concentrations of polycyclic aromatic hydrocarbons eliciting mortality from exposures during the sensitive periods of development were below or near concentrations measured during the active spill phase. Environ Toxicol Chem 2017;36:1887–1895. © 2016 SETAC
The Impact of Regression to the Mean on Economic Evaluation in Quasi‐Experimental Pre–Post Studies: The Example of Total Knee Replacement Using Data from the Osteoarthritis Initiative
Authors: Schilling Chris , Petrie Dennis , Dowsey Michelle M. , Choong Peter F. , Clarke Philip 
Published: January 2017

AbstractMany treatments are evaluated using quasi‐experimental pre–post studies susceptible to regression to the mean (RTM). Ignoring RTM could bias the economic evaluation. We investigated this issue using the contemporary example of total knee replacement (TKR), a common treatment for end‐stage osteoarthritis of the knee.Data (n = 4796) were obtained from the Osteoarthritis Initiative database, a longitudinal observational study of osteoarthritis. TKR patients (n = 184) were matched to non‐TKR patients, using propensity score matching on the predicted hazard of TKR and exact matching on osteoarthritis severity and health‐related quality of life (HrQoL). The economic evaluation using the matched control group was compared to the standard method of using the pre‐surgery score as the control.Matched controls were identified for 56% of the primary TKRs. The matched control HrQoL trajectory showed evidence of RTM accounting for a third of the estimated QALY gains from surgery using the pre‐surgery HrQoL as the control. Incorporating RTM into the economic evaluation significantly reduced the estimated cost effectiveness of TKR and increased the uncertainty. A generalized ICER bias correction factor was derived to account for RTM in cost‐effectiveness analysis.RTM should be considered in economic evaluations based on quasi‐experimental pre–post studies. Copyright © 2017 John Wiley & Sons, Ltd.
Synthesis and Biochemical Evaluation of Biotinylated Conjugates of Largazole Analogues: Selective Class I Histone Deacetylase Inhibitors
Authors: Zhao Le , Dunne Christine E. , Clausen Dane J. , Roberts Justin M. , Paulk Joshiawa , Liu Haining , Wiest Olaf G. , Bradner James E. , Williams Robert M. 
Published: January 2017

AbstractThe synthesis of biotinylated conjugates of synthetic analogues of the potent and selective histone deacetylase (HDAC) inhibitor largazole is reported. The thiazole moiety of the parent compound's cap group was derivatized to allow the chemical conjugation to biotin. The derivatized largazole analogues were assayed across a panel of HDACs 1–9 and retained potent and selective inhibitory activity towards the class I HDAC isoforms. The biotinylated conjugate was further shown to pull down HDACs 1, 2, and 3.
Age‐related changes in pre‐ and post‐conization HPV genotype distribution among women with high‐grade cervical intraepithelial neoplasia
Authors: Giannella Luca , Fodero Cristina , Boselli Fausto , Rubino Teresa , Mfuta Kabala , Prandi Sonia 
Published: January 2017

AbstractObjectiveTo assess the effect of age on pre‐ and post‐conization HPV genotype distribution.MethodsThe present retrospective observational study included consecutive women with high‐grade cervical intraepithelial neoplasia who underwent conization at the Cervical Cancer Screening Centre of Reggio Emilia, Italy, and University Hospital of Modena, Italy, between February 1, 2012, and October 31, 2014. Pre‐conization and 6‐month post‐conization HPV genotyping results were compared between four age groups (<30, 30–39, 40–49, and ≥50 years) and age‐related changes in the HPV genotypes present were evaluated.ResultsThere were 162 patients included. The lowest occurrence of pre‐conization high‐risk and probable high‐risk HPV genotypes was observed among patients aged at least 50 years when compared with younger patients (P=0.017). Conversely, women aged at least 50 years exhibited the highest level of post‐conization high‐risk and probable high‐risk HPV genotypes (P=0.043). Additionally, an increasing incidence of recording identical pre‐ and post‐conization HPV genotypes was associated with increasing age (P=0.024), as was increasing post‐treatment recurrence of cervical intraepithelial neoplasia grade 2+ (P=0.030).ConclusionThe presence of high‐risk and probable high‐risk HPV genotypes was lowest among older patients before conization and was highest among these patients post‐conization; post‐treatment HPV clearance decreased with age and increasing age could be a risk factor for post‐conization recurrence.
Inverted multiscale optical resolution photoacoustic microscopy
Authors: Qi Weizhi , Jin Tian , Rong Jian , Jiang Huabei , Xi Lei 
Published: January 2017

Optical resolution photoacoustic microscopy (ORPAM) is one of the fastest evolving photoacoustic imaging modalities. It not only has a comparable spatial resolution to pure optical microscopies such as epifluorescence microscopy, confocal microscopy, and two‐photon microscopy, but also owns a higher penetration depth. In this study, we report an inverted multiscale (IM)‐ORPAM that utilizes a two‐dimensional galvanometric scanner integrated with two microscopic imaging lenses to achieve rotary‐scanning‐based multiscale imaging. A 15 MHz cylindrically focused ultrasonic transducer is mounted on a motorized rotator to synchronously follow the optical scanning paths. To minimize the loss of signal‐to‐noise ratio, the acoustic focal line is precisely adjusted confocal with the optical focal plane. Black tapes, carbon fibers, and sharp blades are imaged to evaluate the performance of the system, and in vivo multiscale imaging of vasculatures inside the ears and brains of mice is demonstrated using this system.
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Cyclin-Dependent Kinase Inhibitor-1-Deficient Mice are Susceptible to Osteoarthritis Associated with Enhanced Inflammation
Authors: Kihara Shinsuke , Hayashi Shinya , Hashimoto Shingo , Kanzaki Noriyuki , Takayama Koji , Matsumoto Tomoyuki , Chinzei Nobuaki , Iwasa Kenjiro , Haneda Masahiko , Takeuchi Kazuhiro , Nishida Kotaro , Kuroda Ryosuke 
Published: January 2017

ABSTRACT

Osteoarthritis (OA) is a multifactorial disease, and recent data suggested that cell cycle–related proteins play a role in OA pathology. Cyclin-dependent kinase (CDK) inhibitor 1 (p21) regulates activation of other CDKs, and recently, we reported that p21 deficiency induced susceptibility to OA induced by destabilization of the medial meniscus (DMM) surgery through STAT3-signaling activation. However, the mechanisms associated with why p21 deficiency led to susceptibility to OA by the STAT3 pathway remain unknown. Therefore, we focused on joint inflammation to determine the mechanisms associated with p21 function during in vitro and in vivo OA progression. p21-knockout (p21−/−) mice were used to develop an in vivo OA model, and C57BL/6 (p21+/+) mice with the same background as the p21−/− mice were used as controls. Morphogenic changes were measured using micro-CT, IL-1β serum levels were detected by ELISA, and histological or immunohistological analyses were performed. Our results indicated that p21-deficient DMM-model mice exhibited significant subchondral bone destruction and cartilage degradation compared with wild-type mice. Immunohistochemistry results revealed p21−/− mice susceptibility to OA changes accompanied by macrophage infiltration and enhanced MMP-3 and MMP-13 expression through IL-1β-induced NF-κB signaling. p21−/− mice also showed subchondral bone destruction according to micro-CT analysis, and cathepsin K staining revealed increased numbers of osteoclasts. Furthermore, p21−/− mice displayed increased serum IL-1β levels, and isolated chondrocytes from p21−/− mice indicated elevated MMP-3 and MMP-13 expression with phosphorylation of IκB kinase complex in response to IL-1β stimulation, whereas treatment with a specific p-IκB kinase inhibitor attenuated MMP-3 and MMP-13 expression. Our results indicated that p21-deficient DMM mice were susceptible to alterations in OA phenotype, including enhanced osteoclast expression, macrophage infiltration, and MMP expression through IL-1β-induced NF-κB signaling, suggesting that p21 regulation may constitute a possible therapeutic strategy for OA treatment. © 2017 American Society for Bone and Mineral Research.
Matrix metalloproteinase‐1 promoter ‐1607 bp 1G/2G polymorphism associated with increased risk of spinal tuberculosis in Southern Chinese Han population
Authors: Zhou Ying , Gao Qile , He Dan , Deng Ang , Huang Rongfu , Li Yanbing , Tan Chunyan , Guo Chaofeng , Guo Qiang , Wang Longjie , Yang Guanteng , Zhang Hongqi 
Published: January 2017

BackgroundSpinal tuberculosis is the most common form of musculoskeletal tuberculosis. The expression of matrix metalloproteinase‐1 (MMP‐1) is increased in cells with Mycobacterium tuberculosis infection. MMP‐1 plays a curial role in extracellular matrix degradation during the progression of tuberculosis. Although the 1G/2G polymorphism in MMP‐1‐1607 influences its transcription, its role in spinal tuberculosis remains unknown.MethodsHealthy controls and patients with spinal tuberculosis of Han ethnicity were recruited between January 2010 and May 2016. The MMP‐1‐1607 1G/2G polymorphism was genotyped using the Sequenom mass Array polymorphism analysis system.ResultsThe genotypes of 1G/1G, 1G/2G, and 2G/2G were found in 13.7%, 53.6%, and 32.8% of patients, and 12.2%, 37.4%, and 50.4% of controls, respectively. The 1G/2G genotype were more common in cases than in controls (P=2.05E‐04). The 1G allele showed an association with an increased risk for spinal tuberculosis when compared to 2G allele (P=.004). 1G genotypes, having at least one 1G allele, were associated with the risk of developing spinal tuberculosis (1G/1G+1G/2G vs 2G/2G: OR=2.084, 95%CI=1.401‐3.100, P=2.65E‐04).Conclusion1G genotypes of the MMP‐1‐1607 may be associated with susceptibility to spinal tuberculosis in Southern Chinese Han population.
Sea and land surface temperatures, ocean heat content, Earth's energy imbalance and net radiative forcing over the recent years
Authors: Dieng H. B. , Cazenave A. , Meyssignac B. , von Schuckmann K. , Palanisamy H. 
Published: January 2017

ABSTRACTWe investigate the global mean and regional change of sea surface and land surface temperature over 2003–2013, using a large number of different data sets, and compare with changes observed over the past few decades (starting in 1950). We find that over 2003–2013, both global land surface temperature and global sea surface temperature have increased at a rate significantly lower than over the previous decades. While confirming cooling of eastern tropical Pacific during the last decade as reported in several recent studies, our results show that the reduced rate of change of the 2003–2013 time span is a global phenomenon. GMST short‐term trends since 1950 computed over successive 11‐year windows with 1‐year overlap show important decadal variability that highly correlates with 11‐year trends of the Atlantic Multidecadal Oscillation index. The GMST 11‐year trend distribution is well fitted by a Gaussian function, confirming an unforced origin related to internal climate variability. We evaluate the time derivative of full‐depth ocean heat content to determine the planetary energy imbalance with different approaches: in situ measurements, ocean reanalysis and global sea level budget. For 2003–2013, it amounts to 0.5 +/− 0.1 W m−2, 0.68 +/− 0.1 W m−2 and 0.65 +/− 0.1 W m−2, respectively for the three approaches. Comparing with the Energy Balanced and Filled (EBAF) data of the Clouds and Earth's Radiant Energy Systems (CERES) project, we find significant agreement at interannual scales. Finally, using 15‐year averages of GMST and total ocean heat content rate, we compute the net radiative forcing since 1970 (this start date being constrained by availability of ocean temperature data). Although the uncertainty is quite large because of considerable errors in the climate sensitivity parameter, we find no evidence of decrease in net radiative forcing in the recent years, but rather an increase compared to the previous decades.
Soil residual nitrogen under various crop rotations and cultural practices
Authors: Sainju Upendra M. , Lenssen Andrew W. , Allen Brett L. , Stevens William B. , Jabro Jalal D. 
Published: January 2017

AbstractCrop rotation and cultural practice may influence soil residual N available for environmental loss due to crop N uptake and N immobilization. We evaluated the effects of stacked vs. alternate‐year crop rotations and cultural practices on soil residual N (NH4‐N and NO3‐N contents) at the 0–125 cm depth, annualized crop N uptake, and N balance from 2005 to 2011 in the northern Great Plains, USA. Stacked rotations were durum (Triticum turgidum L.)–durum–canola (Brassica napus L.)–pea (Pisum sativum L.) (DDCP) and durum–durum–flax (Linum usitatissimum L.)–pea (DDFP). Alternate‐year rotations were durum–canola–durum–pea (DCDP) and durum–flax–durum–pea (DFDP). Both of these are legume‐based rotations because they contain legume (pea) in the crop rotation. A continuous durum (CD) was also included for comparison. Cultural practices were traditional (conventional tillage, recommended seeding rate, broadcast N fertilization, and reduced stubble height) and improved (no‐tillage, increased seeding rate, banded N fertilization, and increased stubble height) systems. The amount of N fertilizer applied to each crop in the rotation was adjusted to soil NO3‐N content to a depth of 60 cm observed in the autumn of the previous year. Compared with other crop rotations, annualized crop biomass N was greater with DCDP and DDCP in 2007 and 2009, but was greater with DDFP than DCDP in 2011. Annualized grain N was greater with DCDP than CD, DFDP, and DDFP and greater in the improved than the traditional practice in 2010 and 2011. Soil NH4‐N content was greater with CD than other crop rotations in the traditional practice at 0–5 cm, but was greater with DDCP than CD and DDFP in the improved practice at 50–88 cm. Soil NO3‐N content was greater with CD than other crop rotations at 5–10 cm, but was greater with CD and DFDP than DCDP and DDCP at 10–20, 88–125, and 0–125 cm. Nitrate‐N content at 88–125 and 0–125 cm was also greater in the traditional than the improved practice. Nitrogen balance based on the difference between N inputs and outputs was greater with crop rotations than CD. Increased N fertilization rate increased soil residual N with CD, but legume N fixation increased N balance with crop rotations. Legume‐based crop rotations (all rotations except CD) reduced N input and soil residual N available for environmental loss, especially in the improved practice, by increasing crop N uptake and N immobilization compared with non‐legume monocrop.
In‐situ isothermal micro‐Raman spectroscopy reveals the activation energy of dehydration in α‐FeOOH
Authors: Sendova Mariana , Hosterman Brian D. , Grebe Anthony 
Published: January 2017

A real‐time, isothermal micro‐Raman spectroscopy was developed as a single technique for monitoring the solid state kinetics of dehydration and measuring its activation energy. The technique comprises of acquisition and numerical analysis of a set of isothermal time‐dependent Raman spectra. α‐FeOOH transformation to Fe2O3 in N2 atmosphere was continuously monitored for up to 16 h at three temperatures. Dehydration activation energy of 1.4(1) eV was estimated. The Raman band evolution of two modes with different symmetries (B1g ~ 240 cm−1 and Ag ~ 400 cm−1) and the intensity decay of B1g mode at ~540 cm−1 are analyzed independently utilizing kinetic model‐free approach. The study opens new possibilities for developing versatile solid state chemical and bio‐chemical Raman sensors and advances the field of analytical micro‐Raman spectroscopy. Copyright © 2017 John Wiley & Sons, Ltd.
Lymphocyte to monocyte ratio and neutrophil to lymphocyte ratio are superior inflammation‐based predictors of recurrence in patients with hepatocellular carcinoma after hepatic resection
Authors: Yang Tianbo , Zhu Jiye , Zhao Lei , Mai Kangye , Ye Jiazhou , Huang Shan , Zhao Yinnong 
Published: January 2017

AimThe purpose of this study was to investigate which inflammation‐based marker more accurately predict recurrence in patients receiving hepatectomy for hepatocellular carcinoma (HCC).MethodsA total of 1020 patients was included. The impacts of clinical variables and inflammation‐based markers on disease‐free survival (DFS) were measured by Kaplan‐Meier method. Selected potential prognostic factors were further analyzed in multivariate model. To reduce influences of selection bias and possible confounders, clinical characteristics of patients were balanced by propensity score matching (PSM).ResultsOf the 1020 patients, 881 (86.4%) were male and 323 (31.7%) received major hepatectomy. In multivariate analysis, cirrhosis (HR: 1.49), tumor size (HR: 1.32), tumor number (HR: 1.57), portal vein tumor thrombus (HR: 1.66), microvascular invasion (HR: 1.60), histological grade (HR: 1.82), operation time (HR: 1.50), alpha foetal protein (HR: 1.29), neutrophil to lymphocyte ratio (NLR) (HR: 1.38), and lymphocyte to monocyte ratio (LMR) (HR: 1.51) were independently predictive of DFS. After PSM, 258 and 213 pairs of patients were generated for LMR and NLR, respectively. LMR and NLR were still independent predictors of recurrence for HCC patients receiving hepatectomy.ConclusionBoth LMR and NLR might be preferable independent prognostic factors for DFS in HCC patients undergoing hepatectomy.
Improved local control with an aggressive strategy of preoperative (with or without intraoperative) radiation therapy combined with radical surgical resection for retroperitoneal sarcoma
Authors: Hull Melissa A. , Molina George , Niemierko Andrzej , Haynes Alex B. , Jacobson Alex , Bernstein Karen De Amorim , Chen Yen‐Lin , DeLaney Thomas F. , Mullen John T. 
Published: January 2017

Background and ObjectivesWe sought to examine our outcomes with advanced preoperative and intraoperative radiation therapy (XRT) combined with aggressive en bloc surgical resection of retroperitoneal sarcoma (RPS) as a strategy to minimize the risk of local recurrence (LR).MethodsFrom 2003 to 2013, 46 patients with RPS received preoperative XRT followed by radical en bloc surgical resection, with or without intraoperative electron radiation therapy (IOERT). Clinical and pathologic variables predictive of LR and distant recurrence (DR) were evaluated.ResultsThirty‐seven patients had primary tumors and 80% were intermediate grade or higher. All patients received preoperative XRT to a median dose of 50.4 Gy and underwent complete (R0/R1) tumor resection, and 16 patients received IOERT. After a median follow‐up of 53 months, 33 (72%) patients were disease‐free, and there were 8 (17%) DRs, 2 (4%) abdominal recurrences outside of the XRT field, and 5 (10.9%) LRs. High tumor grade and recurrent disease at presentation were the only factors associated with higher rates of recurrence.ConclusionsExcellent local control can be achieved with a coordinated strategy of preoperative (±intraoperative) XRT combined with aggressive en bloc surgical resection of RPS, but systemic failure remains a problem for higher‐grade tumors.
Two Rate Constant Kinetic Model for the Chromium(III)–EDTA Complexation Reaction by Numerical Simulations
Authors: Perez‐Benito Joaquin F. 
Published: January 2017

ABSTRACTThe complexation reaction of Cr(III) ion in the presence of a large excess of ethylenediaminetetraacetic acid (EDTA) does not follow a pseudo–first‐order kinetics as sometimes suggested. There are two causes for the deviation from this simple behavior: the involvement of a long‐lived intermediate, precluding the application of the steady‐state approximation, and the autoinhibition provoked by the release of hydrogen ions from the organic ligand to the medium as the final Cr(III)–EDTA violet complex is formed. Numerical simulations have allowed obtaining for each kinetic experiment the values of two rate constants, k1 (corresponding to the formation of the long‐lived intermediate from the reactants) and k2 (corresponding to the formation of the final complex product from the long‐lived intermediate), as well as the number of hydrogens liberated per molecule of final complex product formed (Hkin). The results indicate that k1 is associated with a fast step (Ea = 87 ± 4 kJ mol−1) and k2 to a slow step (Ea = 120 ± 2 kJ mol−1), whereas the number of hydrogen ions lies within the range 0 < Hkin < 2 in all the kinetic runs. A mechanism in accordance with the experimental data has been proposed.