bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conducting research through cross national collaboration
Authors: Steinke Mary K. , Rogers Melanie , Lehwaldt Daniela , Lamarche Kimberley 
Published: October 2017

AbstractAimTo explore the collaborative nature of an international research project with other advanced practice nurse researchers and critically analyse the process.BackgroundResearch within the nursing community is recognized internationally as important to ensure that nurses participate in cutting‐edge health care and promote evidence‐based practices, yet there is little detail found in literature on how a successful collaborative relationship is initiated and conducted in advanced practice research.DesignDiscussion paper: The purpose of this paper is to discuss the process of collaboration on a research study among advanced practice nurses from four countries who are members of an international organization.Implications for NursingThe collaborative process in international nursing research can be challenging and rewarding. It is important to remember that there is a relationship between the complexity of the study and the time required to complete it. Keys to success include following established guidelines.ConclusionThis project was a valuable experience in developing collaborative relationships as well as creating partnerships for future research to build on the knowledge gained. The authors' linkages to universities facilitated their participation in the research and completion of the ethical review processes. The use of social media and university resources was indispensable.
Autism and primary care dentistry: parents’ experiences of taking children with autism or working diagnosis of autism for dental examinations
Authors: Thomas Nicole , Blake Sharon , Morris Christopher , Moles David R. 
Published: October 2017

BackgroundAccessing and receiving preventative dental treatment can be difficult for children with autism due to sensory processing disorders and/or challenging behaviours coupled with a reported reluctance by dentists to treat these children.AimTo gather dental experiences of UK parents of children with autism or working diagnosis of autism and explore how they feel primary care dental services can be improved.DesignA total of 17 parents of children with a diagnosis or working diagnosis of autism took part in semi‐structured interviews. Data were analysed thematically.ResultsKey themes identified were flexibility of the dental team and environment, confidence of the parents to advocate for their children's needs, continuity of services and clear referral pathways to specialist services. Cross‐cutting all themes was the value of clear communication. The experiences provide greater understanding of issues such as hyper‐empathy, the dental chair, challenges of the waiting room, perceived medical authority, and the importance of continuation of care.ConclusionIn line with previous research about the importance of family‐centred care, a strong relationship between parents and the whole dental team is essential for children with autism to access dental examinations and have satisfactory experience of care.
Respiratory syncytial virus evaluation among asymptomatic and symptomatic subjects in a university hospital in Sao Paulo, Brazil, in the period of 2009‐2013
Authors: Moreira Luciana Peniche , Watanabe Aripuana Sakurada Aranha , Camargo Clarice Neves , Melchior Thais Boim , Granato Celso , Bellei Nancy 
Published: October 2017

BackgroundThe respiratory syncytial virus (RSV) is recognized as an important cause of respiratory tract infections. Immunocompromised patients, healthcare workers (HCWs) and children contacts are at increased risk of acquiring the infection. However, the impact of asymptomatic infection in transmission has not been well studied. Objectives: this study evaluated the frequency and viral load (VL) of RSV in nasal swab samples of individuals with different risk factors for acquiring infection in a university hospital in Sao Paulo, Brazil.MethodsWe included 196 symptomatic children and their 192 asymptomatic caregivers, 70 symptomatic and 95 asymptomatic HCWs, 43 samples from symptomatic HIV‐positive outpatients, and 100 samples of asymptomatic HIV patients in the period of 2009‐2013.ResultsRSV infection was detected in 10.1% (70/696) of samples, 4.4% (17/387) of asymptomatic patients, and 17.1% (53/309) from symptomatic patients. (P < .0001). The VL of symptomatic patients (4.7 log copies/mL) was significantly higher compared to asymptomatic patients (2.3 log copies/mL). RSV detection among asymptomatic caregivers (6.8%; 13/192) was significantly higher compared to other asymptomatic adults, HIV and HCWs (2.0%; 4/195; P = .0252). A close contact with an infected child at home was an important risk to RSV acquisition [OR 22.6 (95% CI 4.8‐106.7)]. Children who possibly transmitted the virus to their asymptomatic contacts had significantly higher viral load than children who probably did not transmit (P < .0001).ConclusionsAccording to our results, it is important to know if people circulating inside the hospital have close contact with acute respiratory infected children.
Use of epidermal skin grafts in chronic wounds: a case series
Authors: Everts Peter AM , Warbout Marco , de Veth Diana , Cirkel Merel , Spruijt Nicole E , Buth Jaap 
Published: October 2017

AbstractIn stalled, chronic wounds, more aggressive and proactive wound closure efforts are needed. We describe adjunctive use of epidermal grafting in patients with chronic wounds. Wound bed preparation consisted of surgical necrotectomy or sharp debridement, hyperbaric oxygen therapy, negative pressure wound therapy, compression therapy, platelet‐rich plasma therapy and/or heparan sulphate agents. Epidermal grafts were harvested from the patient's thigh and applied to the wound. Wound and donor site healing was monitored. A total of 78 patients (average age = 64·1 ± 15·6 years) were included in the study. Common comorbidities included hypertension (47·4%), venous insufficiency (37·2%) and obesity (28·2%). Average wound duration was 13·2 months (range: 0·3–180 months). The most common wound types were dehiscence (29·5%), radiation ulcer (24·4%) and venous ulcer (17·9%). Total time from epidermal grafting to wound closure was 10·0 ± 7·3 weeks. Of the 78 wounds, 66 (84·6%) reached full wound closure (49 < 3 months, 16 > 3 months, 1 without time data). Of 78 wounds, 10 (12·8%) underwent partial wound healing, while 2 wounds (2/78; 2·6%) remained unhealed. These results suggest that wound surface reduction can be achieved by proactive early application of biological therapies and epidermal skin grafts, which may help decrease time to wound healing.
Atrial tachycardias: Cause or effect with ablation of persistent atrial fibrillation?
Authors: Yamashita Seigo , Hooks Darren A. , Shah Ashok , Relan Jatin , Cheniti Ghassen , Kitamura Takeshi , Berte Benjamin , Mahida Saagar , Sellal Jean‐Marc , Jefairi Nora Al , Frontera Antonio , Amraoui Sana , Collotand Florent , Denis Arnaud , Derval Nicolas , Sacher Frédéric , Cochet Hubert , Dubois Rémi , Hocini Mélèze , Haïssaguerre Michel , Klein George , Jaïs Pierre 
Published: October 2017

AbstractIntroductionIt is largely believed that atrial tachycardias (ATs) encountered during ablation of persistent atrial fibrillation (PsAF) are a byproduct of ablative lesions. We aimed to explore the alternative hypothesis that they may be a priori drivers of AF remaining masked until other AF sources are reduced or eliminated.Methods and resultsRadiofrequency ablation of fibrillatory drivers mapped by electrocardiographic imaging (ECGI; ECVUE™, Cardioinsight Technologies, Cleveland, OH, USA) terminated PsAF in 198 (73%) out of 270 patients (61 ± 10 years, 9 ± 9 m). Two hundred and six ATs in 158 patients were subsequently mapped. Their anatomic relationship to the fibrillatory drivers prospectively identified by ECGI was then established. There were 26 (13%), 52 (25%), and 128 (62%) focal, localized, and macrore‐entrant ATs, respectively. In focal/localized re‐entrant ATs, 64 (82%) were terminated within an AF‐driver region, in which 26 (81%) among 32 focal/localized ATs analyzed with 3‐D‐mapping system merged to driver map occurred from AF‐driver regions in 1.0 ± 1.0 cm distance from the driver core. Importantly, there was no attempt at ablation of the associated AF‐driver region in 25 of 64 (39%) of focal/localized re‐entrant ATs. The sites of ATs origin generally had low‐voltage, fractionated, and long‐duration electrograms in AF. All but two focal/localized re‐entrant ATs were successfully ablated.ConclusionThe majority of post‐AF‐ablation focal and localized re‐entrant ATs originate from the region of prospectively established AF‐driver regions. A third of these are localized to regions not subsequently submitted to ablation. These data suggest that many ATs exist, although not necessarily manifest independently, prior to ablation. They may have a role in the maintenance of PsAF in these individuals.
Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells
Authors: Tian Zheng‐Wei , Xu Dan‐Hua , Wang Tian‐Yun , Wang Xiao‐Yin , Xu Hong‐Yan , Zhao Chun‐Peng , Xu Guang‐Hua 
Published: October 2017

AbstractLow‐level and unstable transgene expression are common issues using the CHO cell expression system. Matrix attachment regions (MARs) enhance transgene expression levels, but additional research is needed to improve their function and to determine their mechanism of action. MAR‐6 from CHO chromosomes actively mediates high and consistent gene expression. In this study, we compared the effects of two new MARs and MAR‐6 on transgene expression in recombinant CHO cells and found one potent MAR element that can significantly increase transgene expression. Two MARs, including the human CSP‐B MAR element and DHFR intron MAR element from CHO cells, were cloned and inserted downstream of the poly(A) site in a eukaryotic vector. The constructs were transfected into CHO cells, and the expression levels and stability of eGFP were detected by flow cytometry. The three MAR sequences can be ranked in terms of overall eGFP expression, in decreasing order, as follows: human CSP‐B, DHFR intron MAR element and MAR‐6. Additionally, as expected, the three MAR‐containing vectors showed higher transfection efficiencies and transient transgene expression in comparison with those of the non‐MAR‐containing vector. Bioinformatics analysis indicated that the NFAT and VIBP elements within MAR sequences may contribute to the enhancement of eGFP expression. In conclusion, the human CSP‐B MAR element can improve transgene expression and its effects may be related to the NFAT and VIBP elements.
Cell death‐inducing DFF45‐like effector C gene silencing alleviates pulmonary vascular remodeling in a type 2 diabetic rat model
Authors: Sui Dong‐xin , Zhou Hui‐min , Wang Feng , Zhong Ming , Zhang Wei , Ti Yun 
Published: October 2017

AbstractAims/IntroductionCell death‐inducing DFF45‐like effector C (CIDEC) was proven to be closely associated with the development of insulin resistance and metabolic syndrome. We aimed to investigate whether CIDEC gene silencing could alleviate pulmonary vascular remodeling in a type 2 diabetes rat model.Materials and MethodsWe built a type 2 diabetes rat model. An adenovirus harboring CIDEC small interfering ribonucleic acid was then injected into the jugular vein to silence the CIDEC gene. After hematoxylin–eosin and Sirius red staining, we detected indexes of the pulmonary arterioles remodeling. Immunohistochemical staining of proliferating cell nuclear antigen was used to evaluate the pulmonary arterial smooth muscle cell proliferation. Apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling reaction and western blotting. The levels of signaling pathway proteins expression were measured by western blotting analyses.ResultsHistological analysis of the pulmonary artery showed that the thickness of the adventitia and medial layer increased notably in type 2 diabetes rats. Immunohistochemistry showed that more proliferating cell nuclear antigen‐positive pulmonary arterial smooth muscle cells could be seen in type 2 diabetes rats; and after CIDEC gene silencing, proliferating cell nuclear antigen positive cells decreased accordingly. Cleaved caspase‐3 and cleaved poly (adenosine diphosphate‐ribose) polymerase measured by western blotting showed increased apoptosis with overexpressed CIDEC in diabetes. Terminal deoxynucleotidyl transferase dUTP nick end labeling reaction showed that the apoptosis mainly occurred in endothelial cells. Western blotting analysis showed CIDEC overexpression in rats with diabetes, and phosphorylated adenosine 5′ monophosphate‐activated protein kinase‐α expression was significantly decreased. After CIDEC gene silencing, the expression of phosphorylated adenosine 5′ monophosphate‐activated protein kinase‐α was upregulated.ConclusionsThe CIDEC/5′ monophosphate‐activated protein kinase signaling pathway could be a potential therapeutic candidate against pulmonary vascular diseases in type 2 diabetes patients.
Interpreting the Chinese version of quality of life questionnaire for functional digestive disorders
Authors: Hou Zheng‐kun , Mi Hong , Liu Feng‐bin , Chen Zhuo‐qun , Chen Xin‐lin , Wu Yu‐hang , Che Xiao‐lu 
Published: October 2017

AbstractBackground and AimThe Chinese version quality of life questionnaire for functional digestive disorders (Chin‐FDDQL) is a useful health assessment instrument for functional dyspepsia. This study aims to identify its score interpretation for clinical practice.MethodsData of Chin‐FDDQL from the functional dyspepsia patients (≥ 18 years) between November 2009 and April 2013 were enrolled in the 1st and 14th day. After baseline and responsiveness analysis, the single score interpretation and percentile ranks were established. The statistically reliable change was defined with effect size, standardized response mean, minimal detectable change, and others. Then the receiver operating characteristic curve analysis for health improvement was performed to define the clinically important change.ResultsTwo hundred two functional dyspepsia patients, 150 healthy participants, and 25 missing data were enrolled for analysis. Compared with the intake patients, the discharged and healthy persons have significant better health status in all domains (P < 0.001, expect discomfort in discharged people, P = 0.142), totally contrast to missing data. The reliability for single total intake and discharge were both ± 1. Based on score distribution, the 25th, 50th, and 75th percentile ranks were 49, 58, and 66 for intake scores and 59, 65, and 72 for discharge scores, respectively. The minimal detectable change and Reliable Change Index were 6 and 11 for total score. Receiver operating characteristic analyses supported that total score changes 4 or more represented minimal clinically important improvement.ConclusionsThe score interpretation system of the Chin‐FDDQL could assist clinician's decision making during the therapy practice.
Humility, Forgiveness, and Emerging Adult Female Romantic Relationships
Authors: Bell Chance A. , Fincham Frank D. 
Published: October 2017

Among a sample of emerging adult females (N = 152) we empirically examined the role of humility and forgiveness in romantic relationships. We specifically tested a model linking perceived humility to relationship satisfaction with self‐forgiveness and partner‐forgiveness. Participants in a romantic relationship completed measures of self‐reported humility, self‐forgiveness, partner‐forgiveness, and relationship satisfaction. Serial mediation analyses were conducted using path analysis to test the following sequence, humilityself‐forgivenesspartner‐forgivenessrelationship satisfaction. Findings indicate that humility was related to relationship satisfaction via a serially mediated path of self‐forgiveness and partner‐forgiveness, which was not reducible to impression management. We consider implications for research and clinical practice.
Effects of GABA active steroids in the female brain with a focus on the premenstrual dysphoric disorder
Authors: Bixo M. , Johansson M. , Timby E. , Michalski L. , Bäckström T. 
Published: October 2017

AbstractPremenstrual dysphoric disorder (PMDD) afflicts 3%‐5% of women of childbearing age, and is characterised by recurrent negative mood symptoms (eg, irritability, depression, anxiety and emotional lability) during the luteal phase of the menstrual cycle. The aetiology of PMDD is unknown, although a temporal association with circulating ovarian steroids, in particular progesterone and its metabolite allopregnanolone, has been established during the luteal phase. Allopregnanolone is a positive modulator of the GABAA receptor: it is sedative in high concentrations but may precipitate paradoxical adverse effects on mood at levels corresponding to luteal phase concentrations in susceptible women. Saccadic eye velocity (SEV) is a measure of GABAA receptor sensitivity; in experimental studies of healthy women, i.v. allopregnanolone decreases SEV. Women with PMDD display an altered sensitivity to an i.v. injection of allopregnanolone compared to healthy controls in this model. In functional magnetic resonance imaging (fMRI) studies, women with PMDD react differently to emotional stimuli in contrast to controls. A consistent finding in PMDD patients is increased amygdala reactivity during the luteal phase. Post‐mortem studies in humans have revealed that allopregnanolone concentrations vary across different brain regions, although mean levels in the brain also reflect variations in peripheral serum concentrations. The amygdala processes emotions such as anxiety and aggression. This is interesting because allopregnanolone is detected at high concentrations within the region into which marked increases in blood flow are measured with fMRI following progesterone/allopregnanolone administration. Allopregnanolone effects are antagonised by its isomer isoallopregnanolone (UC1010), which significantly reduces negative mood symptoms in women with PMDD when administered s.c. in the premenstrual phase. This was shown in a randomised, placebo‐controlled clinical trial in which the primary outcome was change in symptom scoring on the Daily Rating of Severity of Problems (DRSP): the treatment reduced negative mood scores (P < .005), as well as total DRSP scores (P < .01), compared to placebo in women with PMDD. In conclusion, the underlying studies of this review provide evidence that allopregnanolone is the provoking factor behind the negative mood symptoms in PMDD and that isoallopregnanolone could ameliorate the symptoms as a result of its ability to antagonise the allopregnanolone effect on the GABAA receptor.
Relevance of smoking interventions for dental clinic patients with smoking‐related disease
Authors: Bilodeau Elizabeth A. , Guggenheimer James 
Published: October 2017

AbstractObjectivesDespite a decline in cigarette smoking in the United States, high rates persist among the socioeconomically underserved who consequently are at risk for smoking‐related disease (SRD). Since academically affiliated dental clinics are more likely to encounter underserved patients, smoking interventions could address both the oral and systemic risks of continued smoking. To determine the relevance of providing smoking counseling in the context of SRD, this study examined the prevalence of smoking and its associations with socioeconomic status (SES), SRD and its sequela, and medication use.MethodsSocioeconomic and smoking status was determined from 1,797 electronic health records of a sample of patients at a Pennsylvania dental clinic in 2010. Low SES included patients who were covered by a Medicaid program (MA) or “self‐payers.” High SES encompassed those with an employment‐based commercial dental insurance (COM). Self‐reported smoking rates were compared with patients' SES, SRDs or sequela, and medications being used for the management of their SRDs.ResultsOverall, 41.7 percent of these patients were smoking. Smoking was related to SES with the highest rate (52.7 percent) among MA patients compared with 31.5 percent in patients with COM. In addition, 37 percent of patients with SRD or sequela and 33 percent who were taking medications for their management were smoking.ConclusionsAcademically affiliated dental clinics are more likely to encounter underserved patients who smoke and have SRD. For greater patient impact and receptivity, it is essential that tobacco cessation interventions emphasize the risks of smoking on systemic as well as oral health.
Methyl jasmonate: a phytohormone with potential for the treatment of inflammatory bowel diseases
Authors: Besson Jean Carlos Fernando , de Carvalho Picoli Caroline , Matioli Graciette , Natali Maria Raquel Marçal 
Published: October 2017

Abstract

Objectives
The phytohormone methyl jasmonate (MeJA) has been identified as a vital cell regulator in plants. This substance is analogous to eicosanoids and similar to that of anti-inflammatory prostaglandins. In animals and in animal cells, it displayed an efficient neuroprotective, anti-inflammatory and antioxidant action; while in tumoral strains, it demonstrates a potentially highly attractive mechanism of apoptosis induction through various cellular and molecular mechanisms. The aim of the present review was to explore two new hypotheses that explain the action of MeJA, a lipid phytohormone and its potentially anti-apoptotic mechanism for use as a therapeutic target for future treatment of Inflammatory bowel diseases (IBDs).

Key findings
Methyl jasmonate is a new candidate for the treatment of IBDs, modulating the expression of the major classes of caspase-type protease families that selectively act on the extrinsic and intrinsic pathways of the apoptotic process. Its action is based on the reduction of the expression in tumour necrosis factor tissue levels and the modulating action of reactive oxygen species production, acting only on the destruction of cells that express the diseased phenotype, and preserving cells that are not transformed.

Conclusions
Methyl jasmonate may represent an alternative for the transduction processes of important signals in the cellular renewal of the intestinal mucosa.
Molecular determinants of beta‐adrenergic signaling to voltage‐gated K+ channels in the cerebral circulation
Authors: Rhee Sung W. , Rusch Nancy J. 
Published: October 2017

AbstractVoltage‐gated K+ (Kv) channels are major determinants of membrane potential in vascular smooth muscle cells (VSMCs) and regulate the diameter of small cerebral arteries and arterioles. However, the intracellular structures that govern the expression and function of vascular Kv channels are poorly understood. Scaffolding proteins including postsynaptic density 95 (PSD95) recently were identified in rat cerebral VSMCs. Primarily characterized in neurons, the PSD95 scaffold has more than 50 known binding partners, and it can mediate macromolecular signaling between cell‐surface receptors and ion channels. In cerebral arteries, Shaker‐type Kv1 channels appear to associate with the PSD95 molecular scaffold, and PSD95 is required for the normal expression and vasodilator influence of members of this K+ channel gene family. Furthermore, recent findings suggest that the β1‐subtype adrenergic receptor is expressed in cerebral VSMCs and forms a functional vasodilator complex with Kv1 channels on the PSD95 scaffold. Activation of β1‐subtype adrenergic receptors in VSMCs enables protein kinase A‐dependent phosphorylation and opening of Kv1 channels in the PSD95 complex; the subsequent K+ efflux mediates membrane hyperpolarization and vasodilation of small cerebral arteries. Early evidence from other studies suggests that other families of Kv channels and scaffolding proteins are expressed in VSMCs. Future investigations into these macromolecular complexes that modulate the expression and function of Kv channels may reveal unknown signaling cascades that regulate VSMC excitability and provide novel targets for ion channel‐based medications to optimize vascular tone.