bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brief Report: Anti–RNPC‐3 Antibodies As a Marker of Cancer‐Associated Scleroderma
Authors: Shah Ami A. , Xu George , Rosen Antony , Hummers Laura K. , Wigley Fredrick M. , Elledge Stephen J. , Casciola‐Rosen Livia 
Published: February 2017

ObjectivePrior studies have demonstrated an increased risk of cancer‐associated scleroderma in patients with anti–RNA polymerase III (anti–RNAP III) autoantibodies as well as in patients who are triple‐negative for anticentromere (anti‐CENP), anti–topoisomerase I (anti–topo I), and anti–RNAP III (also known as anti‐POL) autoantibodies (referred to as CTP negative). In a recent study of 16 CTP‐negative scleroderma patients with coincident cancer, 25% of the patients were found to have autoantibodies to RNPC‐3, a member of the minor spliceosome complex. This investigation was undertaken to validate the relationship between anti–RNPC‐3 antibodies and cancer and examine the associated clinical phenotype in a large sample of scleroderma patients.MethodsScleroderma patients with cancer were assayed for anti‐CENP, anti–topo I, anti–RNAP III, and anti–RNPC‐3 autoantibodies. Disease characteristics and the cancer–scleroderma interval were compared across autoantibody groups. The relationship between autoantibody status and cancer‐associated scleroderma was assessed by logistic regression.ResultsOf 318 patients with scleroderma and cancer, 70 (22.0%) were positive for anti–RNAP III, 54 (17.0%) were positive for anti–topo I, and 96 (30.2%) were positive for anti‐CENP. Twelve patients (3.8% of the overall group or 12.2% of CTP‐negative patients) were positive for anti–RNPC‐3. Patients with anti–RNPC‐3 had a short cancer–scleroderma interval (median 0.9 years). Relative to patients with anti‐CENP, patients with anti–RNPC‐3 and those with anti–RNAP III had a >4‐fold increased risk of cancer within 2 years of scleroderma onset (for anti–RNPC‐3–positive patients, odds ratio [OR] 4.3, 95% confidence interval [95% CI] 1.10–16.9 [P = 0.037]; for anti–RNAP III–positive patients, OR 4.49, 95% CI 1.98–10.2 [P < 0.001]). Patients with anti–RNPC‐3 had severe restrictive lung disease, gastrointestinal disease, Raynaud's phenomenon, and myopathy.ConclusionAnti–RNPC‐3 autoantibodies, similar to anti–RNAP III autoantibodies, are associated with an increased risk of cancer at the onset of scleroderma. These data suggest the possibility of cancer‐induced autoimmunity in this subset of patients with scleroderma.
Pathogenesis of Diffuse Alveolar Hemorrhage in Murine Lupus
Authors: Zhuang Haoyang , Han Shuhong , Lee Pui Y. , Khaybullin Ravil , Shumyak Stepan , Lu Li , Chatha Amina , Afaneh Anan , Zhang Yuan , Xie Chao , Nacionales Dina , Moldawer Lyle , Qi Xin , Yang Li‐Jun , Reeves Westley H. 
Published: February 2017

ObjectiveDiffuse alveolar hemorrhage (DAH) in lupus patients confers >50% mortality, and the cause is unknown. We undertook this study to examine the pathogenesis of DAH in C57BL/6 mice with pristane‐induced lupus, a model of human lupus‐associated DAH.MethodsClinical/pathologic and immunologic manifestations of DAH in pristane‐induced lupus were compared with those of DAH in humans. Tissue distribution of pristane was examined by mass spectrometry. Cell types responsible for disease were determined by in vivo depletion using clodronate liposomes and antineutrophil monoclonal antibodies (anti–Ly‐6G). The effect of complement depletion with cobra venom factor (CVF) was examined.ResultsAfter intraperitoneal injection, pristane migrated to the lung, causing cell death, small vessel vasculitis, and alveolar hemorrhage similar to that seen in DAH in humans. B cell–deficient mice were resistant to induction of DAH, but susceptibility was restored by infusing IgM. C3−/− and CD18−/− mice were also resistant, and DAH was prevented in wild‐type mice by CVF. Induction of DAH was independent of Toll‐like receptors, inflammasomes, and inducible nitric oxide. Mortality was increased in interleukin‐10 (IL‐10)–deficient mice, and pristane treatment decreased IL‐10 receptor expression in monocytes and STAT‐3 phosphorylation in lung macrophages. In vivo neutrophil depletion was not protective, while treatment with clodronate liposomes prevented DAH, which suggests that macrophage activation is central to DAH pathogenesis.ConclusionThe pathogenesis of DAH involves opsonization of dead cells by natural IgM and complement followed by complement receptor–mediated lung inflammation. The disease is macrophage dependent, and IL‐10 is protective. Complement inhibition and/or macrophage‐targeted therapies may reduce mortality in lupus‐associated DAH.
Comparative effects of energy restriction and resveratrol intake on glycemic control improvement
Authors: Milton‐Laskibar I. , Aguirre L. , Macarulla M.T. , Etxeberria U. , Milagro F.I. , Martínez J.A. , Contreras J. , Portillo M.P. 
Published: February 2017

AbstractResveratrol (RSV) has been proposed as an energy restriction mimetic. This study aimed to compare the effects of RSV and energy restriction on insulin resistance induced by an obesogenic diet. Any additive effect of both treatments was also analyzed. Rats were fed a high‐fat high‐sucrose diet for 6 weeks. They were then distributed in four experimental groups which were either fed a standard control diet (C), or treated with RSV (30 mg/kg/d), or submitted to energy restriction (R, 15%), or treated with RSV and submitted to energy restriction (RR). A glucose tolerance test was performed, and serum glucose, insulin, fructosamine, adiponectin, and leptin concentrations determined. Muscle triacylglycerol content and protein expression of insulin receptor (IRβ), protein kinase B (Akt), Akt substrate of 160 kDa (AS160) and glucose transporter 4 (GLUT‐4) were measured. In RSV rats, fructosamine concentrations were reduced, HOMA‐IR remained unchanged, but glucose tolerance was improved, without changes in phosphorylation of IRβ, Akt, and AS160 or in GLUT‐4 protein expression. Rats under energy restriction showed an improvement in all the markers related to glycemic control, as well as increased phosphorylation of AS160 and protein expression of GLUT‐4. In rats from RR group the results were similar to R group, with the exception of IRβ and Akt phosphorylation, which were increased. In conclusion, mild energy restriction is more efficient than intake of RSV within a standard balanced diet, and acts by means of a different mechanism from that of RSV. No additive effects between RSV and energy restriction were observed. © 2017 BioFactors, 43(3):371–378, 2017
Electricity generation by Pyrococcus furiosus in microbial fuel cells operated at 90°C
Authors: Sekar Narendran , Wu Chang‐Hao , Adams Michael W.W. , Ramasamy Ramaraja P. 
Published: February 2017

ABSTRACTHyperthermophiles are microorganisms that thrive in extremely hot environments with temperatures near and even above 100°C. They are the most deeply rooted microorganisms on phylogenetic trees suggesting they may have evolved to survive in the early hostile earth. The simple respiratory systems of some of these hyperthermophiles make them potential candidates to develop microbial fuel cells (MFC) that can generate power at temperatures approaching the boiling point. We explored extracellular electron transfer in the hyperthermophilic archaeon Pyrococcus furiosus (Pf) by studying its ability to generate electricity in a two‐chamber MFC. Pf growing in defined medium functioned as an anolyte in a MFC operated at 90°C, generating a maximum current density of 2 A m−2 and a peak power density of 225 mW m−2 without the addition of any external redox mediator. Electron microscopy and electrochemical impedance spectroscopy of the anode with the attached Pf biofilm demonstrated bio‐electrochemical behavior that led to electricity generation in the MFC via direct electron transfer. This proof of concept study reveals for the first time that a hyperthermophile such as Pf can generate electricity in MFC at extreme temperatures. Biotechnol. Bioeng. 2017;114: 1419–1427. © 2017 Wiley Periodicals, Inc.
Unveiling gene trait relationship by cross‐platform meta‐analysis on Chinese hamster ovary cell transcriptome
Authors: Zhao Liang , Fu Hsu‐Yuan , Raju Ravali , Vishwanathan Nandita , Hu Wei‐Shou 
Published: February 2017

ABSTRACTIn the past few years, transcriptome analysis has been increasingly employed to better understand the physiology of Chinese hamster ovary (CHO) cells at a global level. As more transcriptome data accumulated, meta‐analysis on data sets collected from various sources can potentially provide better insights on common properties of those cells. Here, we performed meta‐analysis on transcriptome data of different CHO cell lines obtained using NimbleGen or Affymetrix microarray platforms. Hierarchical clustering, non‐negative matrix factorization (NMF) analysis, and principal component analysis (PCA) accordantly showed the samples were clustered into two groups: one consists of adherent cells in serum‐containing medium, and the other suspension cells in serum‐free medium. Genes that were differentially expressed between the two clusters were enriched in a few functional classes by Database for Annotation, Visualization, and Integrated Discovery (DAVID) of which many were common with the enriched gene sets identified by Gene Set Enrichment Analysis (GSEA), including extracellular matrix (ECM) receptor interaction, cell adhesion molecules (CAMs), and lipid related metabolism pathways. Despite the heterogeneous sources of the cell samples, the adherent and suspension growth characteristics and serum‐supplementation appear to be a dominant feature in the transcriptome. The results demonstrated that meta‐analysis of transcriptome could uncover features in combined data sets that individual data set might not reveal. As transcriptome data sets accumulate over time, meta‐analysis will become even more revealing. Biotechnol. Bioeng. 2017;114: 1583–1592. © 2017 Wiley Periodicals, Inc.
Fundal herniation, strangulation, and fistulization to the left lung after greater curvature gastric plication
Authors: Saikaly Elias , Abi Fadel Fahim , El Asmar Antoine , El Rassi Ziad 
Published: February 2017

Key Clinical MessageGastric plication is not a complication‐free procedure. Herein, we present the first known case of gastric plication complicated by herniation of the gastric fundus into the chest cavity through the esophageal hiatus complicated by strangulation, necrosis, abscess formation, and communication between the gastric lumen and the left lung.
Ni Catalysts Supported on Mesoporous Nanocrystalline Magnesium Silicate in Dry and Steam Reforming Reactions
Authors: Ghods Bahare , Rezaei Mehran , Meshkani Fereshteh 
Published: February 2017

AbstractMesoporous nanocrystalline MgSiO3 with high surface area was synthesized by a hydrothermal method and employed as support in dry and steam reforming of methane. Ni/MgSiO3 catalysts were prepared by an impregnation method and characterized by different techniques. N2 adsorption analysis indicated that addition of nickel shifted the pore size distributions to smaller sizes. Temperature‐programmed reduction analysis revealed that a higher nickel loading enhanced the reducibility of the catalyst. The catalytic performance was improved with increasing the nickel content. The Ni/MgSiO3 catalyst exhibited high stability in dry reforming but methane conversion declined with time‐on‐stream in the steam reforming reaction. Temperature‐programmed oxidation profiles of spent catalysts indicated that the high amount of carbon deposited on the catalyst surface in dry and steam reforming was assigned to whisker‐type carbon.
Formation of By‐products in Pure and Aqueous Acrylic Acid – Kinetic Measurements and Formation Mechanisms
Authors: Pfeifer Christian A. , Scholz Cordula , Vogel Kevin , Drochner Alfons , Vogel Herbert 
Published: February 2017

AbstractDuring acrylic acid (AA) storage, a quality loss occurs due to the formation of by‐products such as diacrylic acid (DiAA), triacrylic acid (TriAA), and higher oligomers. This problem intensifies in the presence of water since the formation rate of oligomers increases and further by‐products such as 3‐hydroxypropionic acid (3‐HPA) and 3‐hydroxydiacrylic acid (3‐HDiAA) are formed. However, water is often essential during storage and transport to raise the flash point or reduce the melting point. In this work, the formation kinetics are investigated for all mentioned components in pure and aqueous AA. The formation mechanisms of 3‐HPA and 3‐HDiAA are assumed as acid‐catalyzed ester hydrolyses of DiAA or TriAA. The introduced reactions are modeled with the measured kinetic data in order to confirm the proposed reactions.
Photoelectrochemical Probing of Cellular Interfaces and Evaluation of Cellular H2S Production Based on In Situ‐Generated CdS‐Enhanced TiO2 Nanotube Heterostructures
Authors: Zhu Yuan‐Cheng , Wang Qian , Zhang Li‐Bin , Zhao Wei‐Wei , Xu Jing‐Juan , Chen Hong‐Yuan 
Published: February 2017

AbstractJudiciously integrated nanostructured materials/cellular interfaces could be applied for various applications including physiological monitoring. Physiological H2S is the third most important endogenously generated gasotransmitter and is believed to be related with many diseases. This work reports the novel photoelectrochemical (PEC) probing of cellular interfaces and the evaluation of cellular H2S production based on in situ‐generated CdS‐enhanced TiO2 nanotube (NT) heterostructures. We chose the TiO2 NT array in particular, owing to its structure of regular oriented vertical NTs (which permits fast directional charge transport) and its substantial inner cavity (which guarantees the highly efficient guest loading and molecule capture). HepG2 cells directly grown on and interfacing with the TiO2 NTs are then used for the activation of the TiO2 NTs through the CdS generated in situ on the TiO2 NTs. Owing to the aforementioned unique properties of TiO2 NTs, even small amounts of in situ‐generated CdS could be detected and result in apparent electrical signals. To the best of our knowledge, such TiO2 NT‐based in situ PEC probing of cellular interfaces and evaluation of H2S cellular production have not previously been reported.
Enhanced Peroxidase‐Like Performance of Gold Nanoparticles by Hot Electrons
Authors: Wang Chen , Shi Yi , Dan Yuan‐Yuan , Nie Xing‐Guo , Li Jian , Xia Xing‐Hua 
Published: February 2017

AbstractEnzyme mimics have been widely used as alternatives to natural enzymes. However, the catalytic performances of enzyme mimics are often decreased due to different spatial structures or absence of functional groups compared to natural enzymes. Here, we report a highly efficient enzyme‐like catalytic performance of gold nanoparticles (AuNPs) by visible‐light stimulation. The enzyme‐like reaction is evaluated by the catalytic reaction of AuNPs oxidizing a typical chromogenic substrate 3,3′,5,5′‐tetramethylbenzydine (TMB) with hydrogen peroxide as an oxidant. From investigations of the wavelength‐dependent reaction rate, radical capture, hole‐donor addition, and dark‐field scattering spectroscopy experiments, it is revealed that the strong plasmonic absorption of AuNPs facilitates generation of hot electrons, which are transfered from AuNPs to the adsorbed reactant molecule, greatly promoting the catalytic performance of the enzyme‐like catalytic reaction. The present work provides a simple method for improving the performance of enzyme mimics, which is expected to find further application in the field of plasmon‐enhanced biocatalysis and biosensors.
Luminescent Bis‐Cyclometalated Gold(III) Complexes with Alkynyl Ligands of Hexaphenylbenzene and Hexabenzocoronene Derivatives and Their Supramolecular Assembly
Authors: Yim King‐Chin , Au Vonika Ka‐Man , Wong Keith Man‐Chung , Yam Vivian Wing‐Wah 
Published: February 2017

AbstractA series of luminescent bis‐cyclometalated gold(III) complexes with different nuclearities and various alkynyl ligands derived from hexaphenylbenzene (HPB) and hexabenzocoronene (HBC) have been synthesized. The energies of the low‐energy metal‐perturbed intraligand (IL) π–π*(R−C^N^C) absorptions of the HPB–alkynyl gold(III) complexes have been fine‐tuned by attaching various substituent groups to the bis‐cyclometalating ligands. Similarly, the metal‐perturbed 3IL [π→π*(R−C^N^C)] emissions of the complexes show energy shifts according to the electronic nature of the bis‐cyclometalating ligands. On the contrary, the absorption and emission spectra of the HBC–alkynyl gold(III) complex have been assigned as dominated by the IL transitions of the HBC–alkynyl unit, as supported by transient absorption studies. The supramolecular assembly morphologies of the gold(III) complexes have been studied by TEM and SEM, along with comparisons across different complexes based on the molecular structures, and their assembly processes monitored by concentration‐dependent and variable‐temperature 1H NMR spectroscopy studies.
Designed Synthesis of Lipid‐Coated Polyacrylic Acid/Calcium Phosphate Nanoparticles as Dual pH‐Responsive Drug‐Delivery Vehicles for Cancer Chemotherapy
Authors: Wang Xin , Zhang Manjie , Zhang Lingyu , Li Lu , Li Shengnan , Wang Chungang , Su Zhongmin , Yuan Yue , Pan Weisan 
Published: February 2017

AbstractHerein, we report a facile strategy to prepare supported lipid‐bilayer‐coated polyacrylic acid/calcium phosphate nanoparticles (designated as PAA/CaP@SLB NPs) as a new dual pH‐responsive drug‐delivery platform for cancer chemotherapy. The synthesized PAA/CaP NPs exhibited both a high payload of doxorubicin (DOX) and dual pH‐responsive drug‐release properties. Additionally, the coated lipid bilayer had the ability to enhance the cellular uptake of PAA/CaP NPs without affecting the pH‐responsive drug release. Moreover, the blank PAA/CaP@SLB NPs exhibited excellent biocompatibility and the DOX‐loaded PAA/CaP@SLB NPs markedly increased the cellular accumulation of DOX and its cytotoxic effects on HepG‐2 cells. Furthermore, when used to evaluate the in vivo therapeutic efficacy in mice with the hepatocarcinoma cell line (H‐22), the DOX‐loaded PAA/CaP@SLB NPs exhibited superior inhibition of tumor growth compared with the free DOX group. Thus, PAA/CaP@SLB NPs are a promising drug‐delivery vehicle to increase the therapeutic efficacy of anticancer drugs.
Probing Zeolite Crystal Architecture and Structural Imperfections using Differently Sized Fluorescent Organic Probe Molecules
Authors: Hendriks Frank C. , Schmidt Joel E. , Rombouts Jeroen A. , Lammertsma Koop , Bruijnincx Pieter C. A. , Weckhuysen Bert M. 
Published: February 2017

AbstractA micro‐spectroscopic method has been developed to probe the accessibility of zeolite crystals using a series of fluorescent 4‐(4‐diethylaminostyryl)‐1‐methylpyridinium iodide (DAMPI) probes of increasing molecular size. Staining large zeolite crystals with MFI (ZSM‐5) topology and subsequent mapping of the resulting fluorescence using confocal fluorescence microscopy reveal differences in structural integrity: the 90° intergrowth sections of MFI crystals are prone to develop structural imperfections, which act as entrance routes for the probes into the zeolite crystal. Polarization‐dependent measurements provide evidence for the probe molecule's alignment within the MFI zeolite pore system. The developed method was extended to BEA (Beta) crystals, showing that the previously observed hourglass pattern is a general feature of BEA crystals with this morphology. Furthermore, the probes can accurately identify at which crystal faces of BEA straight or sinusoidal pores open to the surface. The results show this method can spatially resolve the architecture‐dependent internal pore structure of microporous materials, which is difficult to assess using other characterization techniques such as X‐ray diffraction.
Short Total Synthesis of (±)‐γ‐Lycorane by a Sequential Intramolecular Acylal Cyclisation (IAC) and Intramolecular Heck Addition Reaction
Authors: Monaco Alessandra , Szulc Blanka R. , Rao Zenobia X. , Barniol‐Xicota Marta , Sehailia Moussa , Borges Bruno M. A. , Hilton Stephen T. 
Published: February 2017

AbstractAn intramolecular acylal cyclisation (IAC) approach to the synthesis of a range of bicyclic heterocycles is reported. As an example of the utility of the IAC reaction, the methodology was applied in a protecting‐group‐free five‐step total synthesis of (±)‐γ‐lycorane, incorporating a new intramolecular Heck addition reaction to generate the pentacyclic core structure of the natural product in good yield.
Direct Synthesis of Highly Designable Hybrid Metal Hydroxide Nanosheets by Using Tripodal Ligands as One‐Size‐Fits‐All Modifiers
Authors: Kuroda Yoshiyuki , Koichi Tatsuyuki , Muramatsu Keisuke , Yamaguchi Kazuya , Mizuno Noritaka , Shimojima Atsushi , Wada Hiroaki , Kuroda Kazuyuki 
Published: February 2017

AbstractInvited for the cover of this issue is the group of Yoshiyuki Kuroda and Kazuyuki Kuroda at the Waseda University in Tokyo. The image depicts how the production of diverse metal hydroxide nanosheets can be standardized in the same way as Sushi. Read the full text of the article at 10.1002/chem.201605698.
Inorganic and Organometallic Molecular Wires for Single‐Molecule Devices
Authors: Tanaka Yuya , Kiguchi Manabu , Akita Munetaka 
Published: February 2017

AbstractInvited for the cover of this issue is the group of Y. Tanaka and M. Akita at the Tokyo Institute of Technology. The image depicts the jigsaw puzzle of inorganic and organometallic molecular wires, in which their functionality can be easily tuned by changing their transition metal centers. Read the full text of the article at 10.1002/chem.201604812.
NHC‐Catalyzed Metathesis and Phosphorylation Reactions of Disulfides: Development and Mechanistic Insights
Authors: Crocker Reece D. , Hussein Mohanad A. , Ho Junming , Nguyen Thanh V. 
Published: February 2017

AbstractThe development of efficient methods for the metathesis and phosphorylation reactions of disulfide compounds is of widespread interest due to their important synthetic utility in polymer, biological, medicinal and agricultural chemistry. Herein, we demonstrate the use of N‐heterocyclic carbenes (NHCs) as versatile organocatalysts to promote these challenging reactions under mild conditions. This metal‐ and oxidant‐free protocol is operationally simple with very short reaction times. The interplay between the nucleophilicity and basicity of NHCs in these reactions were also elucidated by NMR studies and high‐level ab initio calculations.
Effets des conceptions de l'erreur, de la motivation à apprendre et du soutien social sur l'intention de transférer les nouveaux apprentissages : un modèle de médiation modérée
Authors: Lauzier Martin , Mercier Geneviève 
Published: February 2017

AbstractThis study aims to assess whether trainees' errors orientation can influence their motivation to learn and their intention to transfer learning. Data were collected from 275 trainees participating in a corporate training program offered by a Canadian government agency. Results show that the concept of learning by error positively influences trainees' levels of motivation and their intention to transfer learning. Results also confirm the mediating role motivation plays between the concept of learning by error and the intention of transferring learning. Finally, results confirm the moderating role of social support on the relationship between motivation to learn and the intention to transfer learning. Practical and theoretical implications of these results are discussed. Copyright © 2017 ASAC. Published by John Wiley & Sons, Ltd.