bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large-scale map and 3D modelling of the Henryk Arctowski Polish Antarctic Station
Authors: Pasik Mariusz , Kowalska Maria Elżbieta , Łapiński Sławomir , Rajner Marcin , Bakuła Krzysztof 
Published: April 2017

ABSTRACTThis paper presents survey measurements carried out during the 39th Polish Antarctic Expedition to the Henryk Arctowski Polish Antarctic Station in March 2015. The measurements were used to create a map on a 1:500 scale and for 3D modelling of the station buildings and vicinity. The paper also presents the geodetic control network established around the station. We discuss the issue of creating a digital elevation model for the station and its surroundings. The elevation models were generated using terrestrial laser scanning data integrated with Global Navigation Satellite System real time kinematic and tacheometric surveying. The accuracy of these models was estimated using height differences in relation to survey data. The mean height difference was 0.03 m and root mean square error was 0.05 m. Furthermore, an analysis of changes to the coastline was conducted using archival cartographic materials to assess the threat of Admiralty Bay to the station buildings. The results are important for continued scientific activity and safety at Arctowski Station, and may be useful for future research on King George Island.
Innovation, informed consent, health research and the Supreme Court: Montgomery v Lanarkshire – a brave new world?
Authors: Mchale Jean V. 
Published: April 2017

AbstractThe Supreme Court decision in Montgomery v Lanarkshire ([2015] UKSC11) has been hailed as a landmark not least because the Court enshrines the doctrine of informed consent formally into English law for the first time in relation to medical treatment. This paper explores the decision in Montgomery. It examines what its implications may be in the future for the consent process in relation to health research and innovative treatment and whether it may prove a watershed moment leading to changing dialogues and expectations in relation to consent. First, the paper explores the concept of ‘informed consent’ in clinical research as seen through international, Council of Europe and EU instruments. Second, it considers how English law currently governs the provision of information to research participants in the context of clinical research. It questions whether such an approach will be sustainable in the future. Third, it discusses the decision of the UK Supreme Court in Montgomery v Lanarkshire and asks what might be the impact of this Supreme Court decision in the health research context. It asks whether Montgomery may result in new approaches to consent in health research and innovative treatment.
BODY-MASS ESTIMATION IN PALEONTOLOGY: A REVIEW OF VOLUMETRIC TECHNIQUES
Authors: Brassey Charlotte A. 
Published: April 2017

AbstractBody mass is a key parameter for understanding the physiology, biomechanics, and ecology of an organism. Within paleontology, body mass is a fundamental prerequisite for many studies considering body-size evolution, survivorship patterns, and the occurrence of dwarfism and gigantism. The conventional method for estimating fossil body mass relies on allometric scaling relationships derived from skeletal metrics of extant taxa, but the recent application of three-dimensional imaging techniques to paleontology (e.g., surface laser scanning, computed tomography, and photogrammetry) has allowed for the rapid digitization of fossil specimens. Volumetric body-mass estimation methods based on whole articulated skeletons are therefore becoming increasingly popular. Volume-based approaches offer several advantages, including the ability to reconstruct body-mass distribution around the body, and their relative insensitivity to particularly robust or gracile elements, i.e., the so-called ‘one bone effect.’ Yet their application to the fossil record will always be limited by the paucity of well-preserved specimens. Furthermore, uncertainties with regards to skeletal articulation, body density, and soft-tissue distribution must be acknowledged and their effects quantified. Future work should focus on extant taxa to improve our understanding of body composition and increase confidence in volumetric model input parameters.
MORPHOMUSEUM: AN ONLINE PLATFORM FOR PUBLICATION AND STORAGE OF VIRTUAL SPECIMENS
Authors: Lebrun Renaud , Orliac Maëva J. 
Published: April 2017

AbstractSince the early 1990s, methods for the acquisition of three-dimensional (3-D) data and computer-assisted techniques for the visualization of such data have grown increasingly popular among biologists, paleontologists, and paleoanthropologists. However, thus far no standardized repository for complex virtual models based on 3-D digital data of specimens has emerged, whereas the need for researchers to provide access to 3-D models of specimens as well as the pressure imposed on authors by scientific journals to make original 3-D morphological data publicly available have increased. MorphoMuseuM (M3) aims to fill this gap. M3 is both a peer-reviewed scientific journal (M3 Journal) and a virtual specimen repository (M3 Repository). All scientific articles and their associated 3-D models deposited in M3 go through a formal review process. Each published model is given a DOI and a unique identifier code, which should be cited by researchers using this model in their scientific publications. In this paper, we describe the place of M3 among other online repositories for 3-D data, and explain how the growing community of biologists working with 3-D data can benefit from using M3.
CONFOCAL MICROSCOPY APPLIED TO PALEONTOLOGICAL SPECIMENS
Authors: Ball Alexander D. , Goral Tomasz , Kamanli Seyit A. 
Published: April 2017

AbstractConfocal laser scanning microscopy is a well-established optical technique allowing for three-dimensional (3-D) visualization of fluorescent specimens with a resolution close to the diffraction limit of light. Thanks to the availability of a wide range of fluorescent dyes and selective staining using antibodies, the technique is commonly used in life sciences as a powerful tool for studying different biological processes, often at the level of single molecules. However, this type of approach is often not applicable for specimens that are preserved in historical slide collections, embedded in amber, or are fossilized, and cannot be exposed to any form of selective staining or other form of destructive treatment. This usually narrows the number of microscopic techniques that can be used to study such specimens to traditional light microscopy or scanning electron microscopy. However, these techniques have their own limitations and cannot fully reveal 3-D structures within such barely accessible samples. Can confocal microscopy be of any help? The answer is positive, and it is due to the fact that many paleontological specimens exhibit a strong inherent autofluorescence that can serve as an excellent source of emitted photons for confocal microscopy visualizations either through reconstruction of the induced autoflourescent signal from the sample, or through reconstruction of the reflected signal from the sample surface. Here, we describe the workflow and methodology involved in acquiring confocal data from a sample and reprocessing the resulting image stack using the image-processing program imageJ before reconstructing the data using the open-source 3-D rendering program, Drishti. This approach opens new possibilities for using confocal microscopy in a nondestructive manner for visualizing complex paleontological material that has never previously been considered as suitable for this type of microscopic technique.
Common variants in ZMIZ1 and near NGF confer risk for primary dysmenorrhoea
Authors: Li Zhiqiang , Chen Jianhua , Zhao Ying , Wang Yujiong , Xu Jinrui , Ji Jue , Shen Jingyi , Zhang Weiping , Chen Zuosong , Sun Qilin , Mao Lijuan , Cheng Shulin , Yang Bo , Zhang Dongtao , Xu Yufeng , Zhao Yingying , Liu Danping , Shen Yinhuan , Zhang Weijie , Li Changgui , Shen Jiawei , Shi Yongyong 
Published: April 2017

AbstractPrimary dysmenorrhoea, defined as painful menstrual cramps in the absence of pelvic pathology, is a common problem in women of reproductive age. Its aetiology and pathophysiology remain largely unknown. Here we performed a two-stage genome-wide association study and subsequent replication study to identify genetic factors associated with primary dysmenorrhoea in a total of 6,770 Chinese individuals. Our analysis provided evidence of a significant (P<5 × 10−8) association at rs76518691 in the gene ZMIZ1 and at rs7523831 near NGF. ZMIZ1 has previously been associated with several autoimmune diseases, and NGF plays a key role in the generation of pain and hyperalgesia and has been associated with migraine. These findings provide future directions for research on susceptibility mechanisms for primary dysmenorrhoea. Furthermore, our genetic architecture analysis provides molecular support for the heritability and polygenic nature of this condition.
Efficient self-emulsification via cooling-heating cycles
Authors: Tcholakova Slavka , Valkova Zhulieta , Cholakova Diana , Vinarov Zahari , Lesov Ivan , Denkov Nikolai , Smoukov Stoyan K. 
Published: April 2017

AbstractIn self-emulsification higher-energy micrometre and sub-micrometre oil droplets are spontaneously produced from larger ones and only a few such methods are known. They usually involve a one-time reduction in oil solubility in the continuous medium via changing temperature or solvents or a phase inversion in which the preferred curvature of the interfacial surfactant layer changes its sign. Here we harness narrow-range temperature cycling to cause repeated breakup of droplets to higher-energy states. We describe three drop breakup mechanisms that lead the drops to burst spontaneously into thousands of smaller droplets. One of these mechanisms includes the remarkable phenomenon of lipid crystal dewetting from its own melt. The method works with various oil–surfactant combinations and has several important advantages. It enables low surfactant emulsion formulations with temperature-sensitive compounds, is scalable to industrial emulsification and applicable to fabricating particulate drug carriers with desired size and shape.
Wetting Induced Oxidation of Pt-based Nano Catalysts Revealed by In Situ High Energy Resolution X-ray Absorption Spectroscopy
Authors: Cui Yi-Tao , Harada Yoshihisa , Niwa Hideharu , Hatanaka Tatsuya , Nakamura Naoki , Ando Masaki , Yoshida Toshihiko , Ishii Kenji , Matsumura Daiju , Oji Hiroshi , Ofuchi Hironori , Oshima Masaharu 
Published: April 2017

AbstractIn situhigh energy resolution fluorescence detection X-ray absorption spectroscopy (HERFD-XAS) was used to systematically evaluate interactions of H2O and O2adsorbed on Pt and Pt3Co nanoparticle catalysts in different particle sizes. The systematic increase in oxidation due to adsorption of different species (H2O adsorption <O2adsorption <O2 + H2O coadsorption) suggests that cooperative behavior between O2and H2O adsorptions is responsible for the overpotential induced by hydrated species in fuel cells. From the alloying and particle size effects, it is found that both strength of O2/H2O adsorption and their cooperative effect upon coadsorption are responsible for the specific activity of Pt catalysts.
Experience with crossmodal statistics reduces the sensitivity for audio-visual temporal asynchrony
Authors: Habets Boukje , Bruns Patrick , Röder Brigitte 
Published: April 2017

AbstractBayesian models propose that multisensory integration depends on both sensory evidence (the likelihood) and priors indicating whether or not two inputs belong to the same event. The present study manipulated the prior for dynamic auditory and visual stimuli to co-occur and tested the predicted enhancement of multisensory binding as assessed with a simultaneity judgment task. In an initial learning phase participants were exposed to a subset of auditory-visual combinations. In the test phase the previously encountered audio-visual stimuli were presented together with new combinations of the auditory and visual stimuli from the learning phase, audio-visual stimuli containing one learned and one new sensory component, and audio-visual stimuli containing completely new auditory and visual material. Auditory-visual asynchrony was manipulated. A higher proportion of simultaneity judgements was observed for the learned cross-modal combinations than for new combinations of the same auditory and visual elements, as well as for all other conditions. This result suggests that prior exposure to certain auditory-visual combinations changed the expectation (i.e., the prior) that their elements belonged to the same event. As a result, multisensory binding became more likely despite unchanged sensory evidence of the auditory and visual elements.
Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
Authors: Xu Xiahong , Yuan Yuwei , Hu Guixian , Wang Xiangyun , Qi Peipei , Wang Zhiwei , Wang Qiang , Wang Xinquan , Fu Yingchun , Li Yanbin , Yang Hua 
Published: April 2017

AbstractGold nanoparticles (AuNPs) aggregation-based colorimetric biosensing remains a challenge for bacteria due to their large size. Here we propose a novel colorimetric biosensor for rapid detection ofEscherichia coliO157:H7 (E.coliO157:H7) in milk samples based on pH-regulated transformation of dimer/tetramer ofConcanavalinA (Con A) and the Con A-glycosyl recognition. Briefly, antibody-modified magnetic nanoparticles was used to capture and concentrateE.coliO157:H7 and then to label with Con A; pH adjusted to 5 was then applied to dissociate Con A tetramer to release dimer, which was collected and re-formed tetramer at pH of 7 to cause the aggregation of dextran-modified AuNPs. The interesting pH-dependent conformation-transformation behavior of Con A innovated the design of the release from the bacteria surface and then the reconstruction of Con A. Therefore, we realized the sensitive colorimetric biosensing of bacteria, which are much larger than AuNPs that is generally not suitable for this kind of method. The proposed biosensor exhibited a limit of detection down to 41 CFU/mL, short assay time (~95 min) and satisfactory specificity. The biosensor also worked well for the detection in milk sample, and may provide a universal concept for the design of colorimetric biosensors for bacteria and virus.
Neural plasticity in amplitude of low frequency fluctuation, cortical hub construction, regional homogeneity resulting from working memory training
Authors: Takeuchi Hikaru , Taki Yasuyuki , Nouchi Rui , Sekiguchi Atsushi , Kotozaki Yuka , Nakagawa Seishu , Makoto Miyauchi Carlos , Sassa Yuko , Kawashima Ryuta 
Published: April 2017

AbstractWorking memory training (WMT) induces changes in cognitive function and various neurological systems. Here, we investigated changes in recently developed resting state functional magnetic resonance imaging measures of global information processing [degree of the cortical hub, which may have a central role in information integration in the brain, degree centrality (DC)], the magnitude of intrinsic brain activity [fractional amplitude of low frequency fluctuation (fALFF)], and local connectivity (regional homogeneity) in young adults, who either underwent WMT or received no intervention for 4 weeks. Compared with no intervention, WMT increased DC in the anatomical cluster, including anterior cingulate cortex (ACC), to the medial prefrontal cortex (mPFC). Furthermore, WMT increased fALFF in the anatomical cluster including the right dorsolateral prefrontal cortex (DLPFC), frontopolar area and mPFC. WMT increased regional homogeneity in the anatomical cluster that spread from the precuneus to posterior cingulate cortex and posterior parietal cortex. These results suggest WMT-induced plasticity in spontaneous brain activity and global and local information processing in areas of the major networks of the brain during rest.
The Regulatory T-cell Transcription Factor Foxp3 Protects against Crescentic Glomerulonephritis
Authors: Yang Chen , Huang Xiao-Ru , Fung Erik , Liu Hua-Feng , Lan Hui-Yao 
Published: April 2017

AbstractRegulatory T cells (Tregs) have been shown to play a protective role in glomerulonephritis (GN) and Foxp3 is a master transcription factor in Treg development. In this study, we examined the functional role and mechanisms of Foxp3 in a mouse model of accelerated anti-glomerular basement membrane (anti-GBM) GN induced in antigen-primed Foxp3 transgenic (Tg) mice. Compared with littermate of wildtype (WT) mice in which induced severe crescentic GN developed with progressive renal dysfunction, Foxp3 Tg mice had reduced crescent formation, urinary protein excretion, plasma creatinine and decline in creatinine clearance. The protective role of Foxp3 in crescentic GN was associated with a markedly suppressed expression of proinflammatory interleukin-1 beta (IL-1β), tumour necrosis factor-alpha (TNF-α) and monocyte chemoattractant protein 1 (MCP-1), and diminished infiltration of the kidneys by CD3+ T cells and F4/80+ macrophages. Moreover, overexpression of Foxp3 resulted in a significant increase in CD4+ Foxp3+ Tregs systemically and in the diseased kidneys, thereby blunting Th1, Th2, and Th17 responses systemically and in the kidneys. In conclusion, Foxp3 protects against kidney injury in crescentic GN through enhancement of Treg numbers and function, and suppression of Th1, Th2 and Th17 immune responses at the systemic and local tissue levels.
Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection
Authors: Zou Zhong , Huang Kun , Wei Yanmin , Chen Huanchun , Liu Ziduo , Jin Meilin 
Published: April 2017

AbstractDuck enteritis virus (DEV), duck tembusu virus (DTMUV), and highly pathogenic avian influenza virus (HPAIV) H5N1 are the most important viral pathogens in ducks, as they cause significant economic losses in the duck industry. Development of a novel vaccine simultaneously effective against these three viruses is the most economical method for reducing losses. In the present study, by utilizing a clustered regularly interspaced short palindromic repeats (CRISPR)/associated 9 (Cas9)-mediated gene editing strategy, we efficiently generated DEV recombinants (C-KCE-HA/PrM-E) that simultaneously encode the hemagglutinin (HA) gene of HPAIV H5N1 and pre-membrane proteins (PrM), as well as the envelope glycoprotein (E) gene of DTMUV, and its potential as a trivalent vaccine was also evaluated. Ducks immunized with C-KCE-HA/PrM-E enhanced both humoral and cell-mediated immune responses to H5N1 and DTMUV. Importantly, a single-dose of C-KCE-HA/PrM-E conferred solid protection against virulent H5N1, DTMUV, and DEV challenges. In conclusion, these results demonstrated for the first time that the CRISPR/Cas9 system can be applied for modification of the DEV genome rapidly and efficiently, and that recombinant C-KCE-HA/PrM-E can serve as a potential candidate trivalent vaccine to prevent H5N1, DTMUV, and DEV infections in ducks.
Kai-Xin-San series formulae alleviate depressive-like behaviors on chronic mild stressed mice via regulating neurotrophic factor system on hippocampus
Authors: Zhu Yue , Chao Cheng , Duan Xiuzhu , Cheng Xiaoxuan , Liu Pei , Su Shulan , Duan Jinao , Dong Tina Tingxia , Tsim Karl Wah-Keung 
Published: April 2017

AbstractKai-xin-san (KXS) is a famous Chinese medicinal formula applied for treating stress-related psychiatric diseases with the symptoms such as depression, forgetfulness and dizziness. In clinic, the composition ratio of KXS is always varied and KXS series formulae are created. Here, we aim to compare the anti-depressive effect of different ratios of KXS and reveal its action mechanism on regulation of neurotrophic factor system. Firstly, daily intra-gastric administration of chemically standardized extracts of KXS series formulae for seven days significantly alleviated the depressive symptoms of chronic unpredictable mild stressed mice displayed by enhanced sucrose consumptions and decreased immobile time of forced swimming coupled with increased locomotor activities. KXS might fulfill this effect by up-regulating the expressions of NGF, BDNF and Trk receptors in hippocampus, which were confirmed by the treatment of corresponding blockers tPA-stop and K252a. The ratio with higher amounts of Ginseng Radix et Rhizoma and Polygalae Radix exerted most profound effect on anti-depression and regulation enzymes in metabolic pathway of neurotrophic factors. These findings suggested that KXS was beneficial for enhancing supplies, up-regulating receptors, and restoring the dysfunction of metabolic pathway of neurotrophic factors, which might account for its anti-depression effect.
Microcosm experimental evidence that habitat orientation affects phytoplankton-zooplankton dynamics
Authors: Zhang Yunshu , Pan Ying , Chen Hanxiang , Hu Zhuomiao , Sun Shucun 
Published: April 2017

AbstractAlthough spatial ecology has achieved a great success in the passing decades, the importance of habitat orientation has not been well studied, especially for its effects on prey-predator dynamics. Here, we examined the responses of zooplankton activity and grazing rate to habitat orientation and their consequences on the stability of phytoplankton-zooplankton system in a two-factor factorial experiment involving habitat orientation (three levels; small, medium, and large base area, respectively) and habitat size (64 ml and 512 ml) using two algal-grazer systems (Chlorella pyrenoidosa-Daphnia magna and C. pyrenoidosa- Moina micrura). In both systems, grazer density increased with increasing base area for a given chamber volume and with increasing chamber volume for a given orientation in the first 6 days, followed by a dramatic decrease, which corresponded to increasing the amplitude of density fluctuations in both zooplankton and phytoplankton species. Such an algal-grazer dynamics could be accounted for by the greater average swimming ability and grazing rate observed in large-based and large-volumed chambers. Our results demonstrate that habitat orientation affects the zooplankton behavior and population dynamics of both zooplankton and phytoplankton species, which further influences the stability of phytoplankton-zooplankton systems.
A tunable high-pass filter for simple and inexpensive size-segregation of sub-10-nm nanoparticles
Authors: Surawski N. C. , Bezantakos S. , Barmpounis K. , Dallaston M. C. , Schmidt-Ott A. , Biskos G. 
Published: April 2017

AbstractRecent advanced in the fields of nanotechnology and atmospheric sciences underline the increasing need for sizing sub-10-nm aerosol particles in a simple yet efficient way. In this article, we develop, experimentally test and model the performance of a High-Pass Electrical Mobility Filter (HP-EMF) that can be used for sizing nanoparticles suspended in gaseous media. Experimental measurements of the penetration of nanoparticles having diameters down to ca 1nm through the HP-EMF are compared with predictions by an analytic, a semi-empirical and a numerical model. The results show that the HP-EMF effectively filters nanoparticles below a threshold diameter with an extremely high level of sizing performance, while it is easier to use compared to existing nanoparticle sizing techniques through design simplifications. What is more, the HP-EMF is an inexpensive and compact tool, making it an enabling technology for a variety of applications ranging from nanomaterial synthesis to distributed monitoring of atmospheric nanoparticles.