bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights
Authors: Bhardwaj Anuradha , Tomar Pradeep , Nain Vikrant 
Published: October 2024

Abstract

Background
The precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate the underlying mechanisms that affect cleavage efficiencies. By integrating data from Tsai et al. and Kleinsteiver et al., who employed the GUIDE-seq method, we aim to enhance our understanding of the factors influencing CRISPR-Cas9 activity.

Results
Our research analyzed datasets from Tsai et al. and Kleinsteiver et al., standardizing cleavage efficiencies to align with Tsai et al.’s comprehensive dataset. We identified a range of sequence features, including PAM sequence types, nucleotide composition, GC content, chromatin structure, CpG islands, and gene expression levels. Various machine learning models, including Artificial Neural Networks, Support Vector Machines, Naïve Bayes, k-Nearest Neighbors, Logistic Regression, and Extra Trees Classifiers, were developed and evaluated. The Extra Trees Classifier, particularly with class weighting, exhibited robust performance, achieving high accuracy, precision, recall, and F1 scores. SHAP analysis provided insights into feature importance, highlighting the significant factors contributing to model predictions.

Conclusions
The application of machine learning to predict CRISPR-Cas9 off-target effects demonstrates significant potential in enhancing the precision of genome editing. Our findings underscore the importance of considering a diverse range of sequence and genomic features to improve prediction models. The insights gained from this study can inform the development of safer and more effective CRISPR-based applications in medicine, agriculture, and biotechnology. Future work will focus on further refining these models and exploring their applicability across different genomic contexts.
The Role of Cejusc in Promoting Access to Justice and Creating An Environment Conducive to Community Participation in the Search for Consensual Solutions
Authors: Bedê Júnior Américo , Cruz Rômulo Lago e 
Published: October 2024

Objective: The objective of this study is to investigate the importance of Judicial Centers for Conflict Resolution and Citizenship (Cejuscs) in expanding access to justice and modernizing the Brazilian judicial system, focusing on mediation and negotiation as alternatives to the traditional adversarial model.

Theoretical Framework: The research is based on theories of mediation and negotiation, including the Harvard method, and discusses the relevance of these techniques in the current legal context. Additionally, examples from New York's experience are used to enrich the analysis of Cejuscs' role.

Method: The methodology includes a bibliographic analysis of data on Cejuscs and a specific case study in the Vitorino Freire/MA District, where the implementation of a differentiated model focused on consensus building was proposed. The study also covers the structure and operation of Cejuscs, as well as the selection and training of mediators and conciliators.

Results and Discussion: The results indicate that Cejuscs are effective in promoting consensual alternatives for conflict resolution, and their role can be expanded to handle complex collective disputes. The experience in Vitorino Freire/MA highlights the importance of interinstitutional partnerships for the success of the model.

Research Implications: This study suggests that the Cejuscs model can be adapted to meet new challenges, such as mediating collective disputes, offering a more efficient and cost-effective path to conflict resolution.

Originality/Value: The research contributes by highlighting the need to modernize judicial practices in Brazil and proposes an innovative model for Cejuscs’ performance in highly complex contexts.
Impacts on Health, Environment and Quality of Life in the Post-Covid-19 Pandemic Period
Authors: Galvão Mariana Talarico Marçal , Dellalibera-Joviliano Renata 
Published: October 2024

Objective: To investigate how the pandemic and the post-COVID-19 scenario may impact current and future plans in health (medical area), the environment, and the population's quality of life.

Theoretical Framework: In 2019, the world began to live as a hostage to an infectious disease caused by the SARS-CoV-2 coronavirus. As uncertainty reigns, the severity of the situation has shown that it could have social, health, educational, economic, and environmental aspects.

Method: The methodology adopted for this research comprises a bibliographic review based on books, cataloged scientific articles being published on digital platforms including Scientific Library Online (SciELO), Pubmed, Periódico Capes, Cochrane, and Google Scholar, using the descriptors: COVID-19; quality of life; environment; health; and their respective synonyms, in Portuguese and English, published between 2015 and 2024. The exclusion criteria used in this study were duplicate articles in more than one database, with similar titles and that did not cover the topic, as well as articles published before 2015 in Portuguese and English. The main aspects analyzed for inclusion were the year of publication, type of study, language, objectives and main findings, according to the relevance of the original and current topic. Therefore, considering the inclusion and exclusion criteria, we made a cut of works published in the last 9 years and in the end 31 works were selected for the construction of this study.

Results and Discussion: The results obtained revealed that all social spheres were, and may still be, affected unequally by the pandemic, since it is a situation of unknown nature, which is intensified every day by globalization and social inequalities. Direct impacts on the economy and jobs, as well as indirect effects of learning losses among children who are out of school. In terms of health and the impact on quality of life, we found changes in mental health, with an increase in the diagnosis of depression among adults.

Research Implications: The practical and theoretical implications of this research are discussed, providing insights into how the results can be applied or influence practices in the fields of health, environment and education. These implications can cover areas of health promotion, economics and public and private management.

Originality/Value: This study contributes to the literature by addressing a current topic with extreme social relevance that prioritizes deepening knowledge in the area that has changed humanity the most in current times: the COVID-19 Pandemic. Furthermore, it analyzes the real impacts of this historical and unique moment in the global context, with a greater focus on health, the environment and the quality of life of the human population.
PENGARUH BOARD SIZE, BOARD COMPOSITION, DAN LEVERAGE TERHADAP INTELLECTUAL CAPITAL DISCLOSURE
Authors: Witarsa Anthony Claudio , Hastuti Rini Tri 
Published: October 2024

The main purpose of this research is to find empirical proof about the effect of board size, board composition, and leverage on intellectual capital disclosure. The data used in this research were obtained from manufacturing companies that listed on the Indonesian Stock Exchange (IDX). 288 data were gathered from 144 companies in the year of 2020-2021. Purposive sampling method is used to collect the data and panel data regression model is used for hypothesis testing. Software EViews 9 is used for preparing and processing data. The Results of this research is reach a conclusion that the number of board composition is positive and have not give a significant effect on intellectual capital disclosure. Variable board size and leverage has a negative and dont have a significant effect on intellectual capital disclosure.
FAKTOR YANG MEMPENGARUHI NILAI PERUSAHAAN DENGAN MEDIASI KINERJA PERUSAHAAN
Authors: Andreas Jonathan , Hadi Hadi 
Published: October 2024

The Purpose of this study is to determine the impact of Board Independence, Foreign Ownership, and Capital Structure on Firm Value with the Mediation of Firm Performance. This Research uses data from manufacturers company listed on the Indonesia Stock Exchange from 2018 to 2020. The sample was selected using Purposive Sampling and the results are 21 Companies. The data was processed by SmartPLS 3.3.9 application. The results of this research showed that Board Independence has a negative and significant impact on firm value, Foreign Ownership has a positive and significant impact on firm value, Capital Structure has a positive but not significant impact on firm value,Board Independence and Foreign Ownership has a positive and significant impact on Firm Performance, Capital Structure has a negative and significant impact on Firm Performance, Board Independence has a positive and significant impact on firm value with firm performance mediation, Foreign ownership has a positive and significant impact on firm value with firm performance mediation, capital structure has a negative and significant impact on firm value with firm performance mediation, and firm performance has a positive and significant impact on firm value.
Faktor-Faktor Yang Mempengaruhi Financial Distress pada Perusahaan Non Keuangan
Authors: Gunawan James Tirta , Nariman Augustpaosa 
Published: October 2024

The purpose of this study is to obtain empirical evidence about the effects of sales growth, operating capacity, and cash flow on financial distress. The sample in this study was selected using purposive sampling method amounted to 31 non finance companies listed on the Indonesia Stock Exchange (IDX) within 2018-2020 that match the criteria. The data processing technique using logistic regression analysis assisted by the EViews 12 program. The results of this study indicate that sales growth has no effect on financial distress, while operating capacity has a negative and significant effect on financial distress, and cash flow has no effect on financial distress.
Material and electronic-digital traces as an element of the forensic characteristics of frauds committed in the way "Your relative got into trouble"
Authors: Revenko Natal'ya Ivanovna 
Published: October 2024

The subject of the study is the material and electronic-digital traces formed during the commission of fraud in the way "Your relative got into trouble". Despite the fact that the fraudster communicates with the victim by phone remotely, when committing fraud, there remains a certain set of material traces, the detection and investigation of which will allow to identify the fraudster courier and prove the event of the crime. The separation of electronic and digital traces into a separate group is associated with the use of information technology by fraudsters. During the investigation, the investigator seizes and examines such an electronic digital trace as a video recording from the victim's place of residence, from the location of the ATM used to transfer money stolen from the victim. Also, this group of traces includes information contained in the cell phones of the victim and the suspect, which confirm the latter's involvement in the crimes committed as part of a criminal group. The study was conducted using general scientific methods of cognition: analysis, synthesis, deduction, induction, analogy; empirical methods: description, comparison, as well as private scientific methods: legal, sociological, formal legal. The novelty of the study, the conclusions. The analysis of criminal cases made it possible to establish that when committing this type of fraud, there are material traces that allow identifying the fraudulent courier, as well as proving the fact of committing a crime. Depending on the method used, specific traces remain, called in criminology as electronic-digital, which include video recordings from surveillance cameras of a fraudster courier at the moment when he came to the victim for money and transferred them to the account indicated to him through an ATM. The author's position is expressed that with the participation of a suspect during the inspection of video recordings, his testimony should be recorded in the interrogation protocol, and not in the protocol of inspection of objects. A large set of traces is contained in the memory of a cell phone seized from a fraudulent courier.
Lean Management Strategies for Creative Organizations: Creacracy versus Bureaucracy
Authors: Zaharov Iurii , Logunova Larisa Yur'evna 
Published: October 2024

The idea to change the traditional hierarchical structure of the company is emerging as a trend in global management practices of creative industries organizations. The experience of American and Western European companies in the "creative industry" shows that a democratic decision-making process promotes the active participation of employees in the life of the company. The topic of creative management, art management, and "lean" management gets a particularly relevant sound. Today, management experience has been accumulated, thanks to which schools, hospitals, creative organizations, design bureaus have been humanized by the talent of employees, vocation and creative achievements have been supported. The subject of research was determined by the growth and demand for companies in the "creative industry" that produce creative products in the socio-cultural, scientific, artistic, and technical fields. The classification of the UK Department of Culture, Media and Sport (DCMS) identifies 44 creative professions, including architecture, IT and video games, publishing, performing arts, crafts, design, advertising, jewelry. The study applied an innovative approach to the management sociology: the experience of creacracy was studied through the prism of culture, the value component of the work of organizations, on the methodological foundation of the "turquoise" philosophy. The study of creacracy culture is based on a set of techniques: value-structural analysis, secondary analysis, document analysis, comparative analysis. Realizing the purpose of the study, the authors theoretically comprehended the experience of modern creative unions and organizations of a non–bureaucratic type based on the foundation of the culture of creative management – creacracy. The authors defined this form of management as the management of organizations, an alternative to bureaucracy, corresponding to a culture based on the values of human-centricity, freedom, creativity, transparency, implemented on the principles of lean or understanding management in the practices of "happy" ("joyful") management of creative projects. A group of types of creacratic management has been formed: adhocratic, chaordic, halordic. With differences in the choice of management tactics, these types are united by the design of management strategies – delegation of authority, coordination, support, orientation towards self-government. This shows the human-centricity of creacratic management. A comparative analysis of bureaucratic and creacratic forms showed differences in values, setting and understanding goals, choosing leading managerial functions, management strategies and practices, and types of employees. The analytics presented in the research results are of practical importance for the heads of creative industries organizations choosing management strategies.
Service design technology as a tool for the development of the Institute of social services
Authors: Sipunova Nadezhda Valentinova 
Published: October 2024

The purpose of this study is to analyze the features of the service design technology in social services. The object of the research is the institutional features of social services for the population, the subject is the role and essence of service design technology as a tool for the development of the institute of social services. The author provides an analysis of the institutional features of the development of social services, examines the impact of the use of service design technologies in the provision of social services. Special attention is paid to the characteristics of innovative social service practices based on the implementation of service design technology, as well as to the analysis of the development and transformation of institutional functions of social services as a result of the widespread introduction of service design practices in the social sphere. Based on the data of the analysis of best practices of social services, the author also analyzes the processes of formation of new social institutions as a result of the implementation of the service approach in the social sphere, one of which is the institute of regional service commissioners. The main research method is the traditional analysis of documents, generalization of the research of the Institute of Social Services, experience in the implementation of service design technology, as well as analysis of its impact on the processes of transformation of social institutions. The theoretical and methodological basis of the research is the works devoted to the study of the peculiarities of the development of the institute of social services, the introduction of service design technology into the processes of service provision. The main results of this study are conclusions about the special role of service design of social services in ensuring the sustainability of public relations by balancing the implementation of public interests associated with the absence of excessive spending on social services and the interests of small groups meeting their needs at an optimal level through convenient routing and individual design of social services. The author's scientific contribution consists in analyzing the transformation of institutional functions of social services in the implementation of service design technology. The author concludes that the institutional function of social services for reproduction, distribution and exchange also changes with the implementation of the service design approach, becoming simpler and more effective, and the structure of public relations is changing under the influence of the need for new actors and institutional forms of social services with an increased role of interdepartmental interaction.
Prohibition of certain actions in the system of preventive measures: practice of application and directions of optimization
Authors: Filimonova Marina Dmitrievna 
Published: October 2024

The subject of this study is the problematic aspects of the legal regulation of the application of the prohibition of certain actions provided for in Article 105.1 of the Criminal Procedure Code of the Russian Federation, as well as law enforcement practice. The introduction of a ban on certain actions in the system of preventive measures actualizes the study of the measure in question in the scientific community, which is fully confirmed by the intensity of publication of relevant works. Meanwhile, the degree of scientific development is insufficiently saturated with analysis of the practice of prohibiting certain actions in comparison with the study of the theoretical and regulatory aspects of this institute, as well as specific proposals for optimizing the legal regulation of the prohibition of certain actions. It can be stated that the research potential of the practice of application and problem analysis of the prohibition of certain actions is quite extensive. The above allows you to focus on the designated aspects.The purpose of the study is to identify problems and conflicts in the regulatory regulation of the prohibition of certain actions, analyze them, develop proposals to minimize them, which will contribute to the expansion of the application of the prohibition of certain actions as a preventive measure. The research methodology is formed taking into account the specifics of the object and subject of research and includes, in addition to general scientific – analysis and synthesis, the method of reviewing scientific research, systemic, problem analysis, – private scientific – formal legal and comparative legal research methods. The scientific novelty is mediated by the variability of the approach to the already identified conflicts of legal regulation of the prohibition of certain actions and the analysis of problems not previously discussed by the scientific community. The conclusions of this study are as follows. Firstly, the legal regulation of the institution of preventive measures needs to be optimized. In particular, the legislator needs to eliminate the existing conflict between the indication of the election of one preventive measure and the possibility of a combination of preventive measures. Secondly, the provisions of Part 4 of Article 105.1 of the Code of Criminal Procedure of the Russian Federation were analyzed, providing for variants of the judge's decisions on the declared petition for the election of a ban on certain actions, according to the results of which amendments and additions were proposed for the uniform and prompt application of this preventive measure. Thirdly, in order to eliminate the double interpretation of the phrase "committed crime" (clause 6, part 6, Article 105.1 of the Code of Criminal Procedure of the Russian Federation), it is proposed to replace the specified wording.
On the prospects of using foreign experience in training personnel for civil society entities in Russia
Authors: Potapenko Sergei Viktorovich , Goncharov Vitalii Viktorovich , Malinovskii Oleg Nikolaevich , Petrenko Elena Gennad'evna , Cheshin Andrei Vladimirovich 
Published: October 2024

This article is devoted to the analysis of modern problems and prospects of using the experience of training workers abroad for various subjects of civil society in the Russian Federation. It is argued that the institute of public control acts as a key legal guarantee for the implementation, protection and protection of both the system of constitutional principles and the entire system of rights, freedoms and legitimate interests of individuals and legal entities in the country. The authors substantiate the position that the basis of the organization and activity of any subject of public control in Russia is its human resources potential. The article identifies several categories of individuals who both take part in the preparation and conduct of public control measures and generally ensure the activities of the above-mentioned subjects of public control. In the course of writing this scientific article, a number of general and particular scientific research methods were used, including: formal-logical; comparative-legal; historical-legal; statistical; sociological; method of analyzing specific legal situations. In the work, special attention is paid to the analysis of the positive experience of training and retraining abroad of representatives of subjects of public control. The authors substantiate why the positive foreign experience of training and retraining of personnel for civil society entities (including in the field of organizing and exercising civil society control over the apparatus of public authority and other objects exercising certain public powers) is important for analysis, adaptation and use in the process of improving the institute of public control in the Russian Federation. The article develops and substantiates a system of measures to optimize the processes of training and advanced training of both members of subjects of public control in Russia and employees of state and municipal institutions that ensure their functioning.