bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic characteristics and phylogenetic analysis of three Chinese ethnic groups using the Huaxia Platinum System
Authors: Wang Mengge , Wang Zheng , He Guanglin , Jia Zhenjun , Liu Jing , Hou Yiping 
Published: January 2018

AbstractShort tandem repeats (STRs) are attractive to genetic applications like forensic, anthropological and population genetics studies. The Huaxia Platinum System was specifically developed to allow co-amplification and detection of all markers in the expanded CODIS core loci and the Chinese National Database. In this study, in continuation to our previous validation study, 568 unrelated individuals were firstly genotyped to investigate the effectiveness of this novel assay in 3 main ethnic groups of China (Han, Tibetan and Yi). The combined power of discrimination (CPD) were 0.9999999999999999999999999992, 0.999999999999999999999999992, 0.999999999999999999999999998, respectively, and the combined power of exclusion (CPE) were 0.9999999999, 0.999999995, 0.999999998, respectively. Next, genetic relationships along administrative and ethnic divisions were analyzed using pairwise genetic distances, multidimensional scaling (MDS), principal component analysis (PCA) and phylogenetic analysis. The Han ethnicity showed a high genetic homogeneity all across China, and significant genetic differences existed between Han groups and some minority groups, most prominently for the Tibetans, the Uyghurs, the Kazakhs, the Miaos, the Zhuangs and the Dais. Aforementioned results suggested that the Huaxia Platinum System is polymorphic and informative, which provides an efficient tool not only for human forensics, but also for population genetics studies.
Physical Limitations of Phosphor layer thickness and concentration for White LEDs
Authors: Tan Cher Ming , Singh Preetpal , Zhao Wenyu , Kuo Hao-Chung 
Published: January 2018

AbstractIncreasing phosphor layer thickness and concentration can enhance the lumen flux of white LED (W-LED). In this work, we found that increasing the phosphor layer thickness and concentration can increase its temperature, and there is also a maximum thickness and concentration beyond which their increase will not lead to lumen increase, but only temperature increase. Higher thickness and higher concentration also results in warm light instead of White light. The maximum thickness and concentration are found to be limited by the scattering of light rays with higher % decrease of blue light rays than the yellow light rays. The results obtained in this work can also be used to compute the temperature and thermo-mechanical stress distribution of an encapsulated LED, demonstrating its usefulness to the design of encapsulated LED packages. Simulation software like ANSYS and TracePro are used extensively to verify the root cause mechanisms.
Presence and molecular characterization of Cryptosporidium and Giardia in recreational lake water in Tianjin, China: a preliminary study
Authors: Xiao Shumin , Zhang Yan , Zhao Xiaoyun , Sun Liping , Hu Sike 
Published: January 2018

AbstractLittle is known about the occurrence of Cryptosporidium and Giardia in recreational water in China. A total of 52 samples were collected from recreational lakes in Tianjin during a high-occurrence season (June–October) for the waterborne cryptosporidiosis and giardiasis, and the occurrence and genotypes of Cryptosporidium and Giardia were investigated. The results showed that 82.7% (43) and 98.1% (51) of samples were positive for Cryptosporidium oocyst and Giardia cysts, respectively. The mean concentration of parasites was 3.65 oocysts/10 L and 12.58 cysts/10 L, respectively. Molecular characterization revealed that the presence of Cryptosporidium parvum, C. andersoni, C. hominis, C. meleagridis, C. fragile, C. ubiquitum, and Giardia lamblia assemblage A, B and D. The protozoan contamination in the studied lakes may originate from animal feces on ground, which was washed into the lake by stormwater runoff. Nevertheless, there is a potential risk of infection during recreational activities in the lake because the dominant detected protozoan genotypes are common human pathogens. Moreover, microbial indicators analysis does not adequately indicate the protozoan contamination in recreational water. The information from this study will be valuable for future protozoan source tracking, and any further control interventions against Cryptosporidium and/or Giardia infection associated with recreational water.
Impact of overexpression of cytosolic isoform of O-acetylserine sulfhydrylase on soybean nodulation and nodule metabolome
Authors: Krishnan Hari B. , Song Bo , Oehrle Nathan W. , Cameron Jeffrey C. , Jez Joseph M. 
Published: January 2018

AbstractNitrogen-fixing nodules, which are also major sites of sulfur assimilation, contribute significantly to the sulfur needs of whole soybean plants. Nodules are the predominant sites for cysteine accumulation and the activity of O-acetylserine(thiol)lyase (OASS) is central to the sulfur assimilation process in plants. Here, we examined the impact of overexpressing OASS on soybean nodulation and nodule metabolome. Overexpression of OASS did not affect the nodule number, but negatively impacted plant growth. HPLC measurement of antioxidant metabolites demonstrated that levels of cysteine, glutathione, and homoglutathione nearly doubled in OASS overexpressing nodules when compared to control nodules. Metabolite profiling by LC-MS and GC-MS demonstrated that several metabolites related to serine, aspartate, glutamate, and branched-chain amino acid pathways were significantly elevated in OASS overexpressing nodules. Striking differences were also observed in the flavonoid levels between the OASS overexpressing and control soybean nodules. Our results suggest that OASS overexpressing plants compensate for the increase in carbon requirement for sulfur assimilation by reducing the biosynthesis of some amino acids, and by replenishing the TCA cycle through fatty acid hydrolysis. These data may indicate that in OASS overexpressing soybean nodules there is a moderate decease in the supply of energy metabolites to the nodule, which is then compensated by the degradation of cellular components to meet the needs of the nodule energy metabolism.
Counseling Issues: Addressing Behavioral and Emotional Considerations in the Treatment of Communication Disorders
Authors: Lieberman Aaron 
Published: January 2018

Purpose
Counseling is an important fundamental professional activity and an established component of the speech-language pathology/audiology scope of practice as documented by the American Speech-Language-Hearing Association (2004a, 2016a). Appropriate incorporation of counseling into practice can optimize service provision, help address comorbid behavioral and emotional reactions to communication disorders, and enhance prognosis. Practitioner insecurity in incorporating counseling into practice has been documented by Phillips and Mendel (2008), as well as Atkins (2007). This tutorial seeks to present general counseling constructs and treatment applications primarily utilizing a humanistic counseling perspective.

Method
This tutorial incorporated relevant publications from communication disorders and counseling psychology literature databases over a 3-year period. The tutorial evolved through interviews with practicing speech-language pathology practitioners and educators and was undertaken to provide transparency and clarity to support the assertions and recommendations offered.

Results
This tutorial organizes and presents general counseling considerations along with specific suggestions, which can help practitioners who are seeking to expand their understanding, skill, and confidence in incorporating counseling into their practice. The tutorial identifies some of the potential and impactful comorbid emotional and behavioral responses to experiencing a communication disorder and/or to the treatment process for the communication disorder issue(s). Theoretically grounded considerations for mitigating such potentially impactful responses are offered.

Conclusion
Continued and enhanced efforts may be necessary to meet American Speech-Language-Hearing Association's mandate for universal incorporation of empirically supported counseling approaches and in advancing universal speech-language pathology/audiology training in this area. It is hoped that this tutorial serves as an initial guide for addressing this need until universal preparation and practice is achieved.
Modelling the carbon AGB star R Sculptoris
Authors: Brunner M. , Maercker M. , Mecina M. , Khouri T. , Kerschbaum F. 
Published: January 2018

Context. On the asymptotic giant branch (AGB), Sun-like stars lose a large portion of their mass in an intensive wind and enrich the surrounding interstellar medium with nuclear processed stellar material in the form of molecular gas and dust. For a number of carbon-rich AGB stars, thin detached shells of gas and dust have been observed. These shells are formed during brief periods of increased mass loss and expansion velocity during a thermal pulse, and open up the possibility to study the mass-loss history of thermally pulsing AGB stars.
Aims. We study the properties of dust grains in the detached shell around the carbon AGB star R Scl and aim to quantify the influence of the dust grain properties on the shape of the spectral energy distribution (SED) and the derived dust shell mass.
Methods. We modelled the SED of the circumstellar dust emission and compared the models to observations, including new observations of Herschel/PACS and SPIRE (infrared) and APEX/LABOCA (sub-millimeter). We derived present-day mass-loss rates and detached shell masses for a variation of dust grain properties (opacities, chemical composition, grain size, and grain geometry) to quantify the influence of changing dust properties to the derived shell mass.
Results. The best-fitting mass-loss parameters are a present-day dust mass-loss rate of 2 × 10−10 M⊙ yr−1 and a detached shell dust mass of (2.9 ± 0.3) × 10−5 M⊙. Compared to similar studies, the uncertainty on the dust mass is reduced by a factor of 4. We find that the size of the grains dominates the shape of the SED, while the estimated dust shell mass is most strongly affected by the geometry of the dust grains. Additionally, we find a significant sub-millimeter excess that cannot be reproduced by any of the models, but is most likely not of thermal origin.
The VMC survey
Authors: Niederhofer Florian , Cioni Maria-Rosa L. , Rubele Stefano , Schmidt Thomas , Bekki Kenji , de Grijs Richard , Emerson Jim , Ivanov Valentin D. , Oliveira Joana M. , Petr-Gotzens Monika G. , Ripepi Vincenzo , Sun Ning-Chen , van Loon Jacco Th. 
Published: January 2018

We use deep multi-epoch point-spread function (PSF) photometry taken with the Visible and Infrared Survey Telescope for Astronomy (VISTA) to measure and analyze the proper motions of stars within the Galactic globular cluster 47 Tucanae (47 Tuc, NGC 104). The observations are part of the ongoing near-infrared VISTA survey of the Magellanic Cloud system (VMC). The data analyzed in this study correspond to one VMC tile, which covers a total sky area of 1.77 deg2. Absolute proper motions with respect to ~9070 background galaxies are calculated from a linear regression model applied to the positions of stars in 11 epochs in the Ks filter. The data extend over a total time baseline of about 17 months. We found an overall median proper motion of the stars within 47 Tuc of (μαcos(δ), μδ) = (+5.89 ± 0.02 (statistical) ± 0.13 (systematic), −2.14 ± 0.02 (statistical) ± 0.08 (systematic)) mas yr−1, based on the measurements of ~35 000 individual sources between 5′ and 42′ from the cluster center. We compared our result to the proper motions from the newest US Naval Observatory CCD Astrograph Catalog (UCAC5), which includes data from the Gaia data release 1. Selecting cluster members ( ~2700 stars), we found a median proper motion of (μαcos(δ), μδ) = (+5.30 ± 0.03 (statistical) ± 0.70 (systematic), −2.70 ± 0.03 (statistical) ± 0.70 (systematic)) mas yr−1. Comparing the results with measurements in the literature, we found that the values derived from the VMC data are consistent with the UCAC5 result, and are close to measurements obtained using the Hubble Space Telescope. We combined our proper motion results with radial velocity measurements from the literature and reconstructed the orbit of 47 Tuc, finding that the cluster is on an orbit with a low ellipticity and is confined within the inner ~7.5 kpc of the Galaxy. We show that the use of an increased time baseline in combination with PSF-determined stellar centroids in crowded regions significantly improves the accuracy of the method. In future works, we will apply the methods described here to more VMC tiles to study in detail the kinematics of the Magellanic Clouds.
Probing the nature of AX J0043−737: Not an 87 ms pulsar in the Small Magellanic Cloud
Authors: Maitra C. , Ballet J. , Esposito P. , Haberl F. , Tiengo A. , Filipović M. D. , Acero F. 
Published: January 2018

Aims. AX J0043−737 is a source in the ASCA catalogue whose nature is uncertain. It is most commonly classified as a Crab-like pulsar in the Small Magellanic Cloud (SMC) following apparent detection of pulsations at ~87 ms from a single ASCA observation. A follow-up ASCA observation was not able to confirm this, and the X-ray detection of the source has not been reported since.
Methods. We studied the nature of the source with a dedicated XMM-Newton observation. We ascertained the source position, searched for the most probable counterpart, and studied the X-ray spectrum. We also analysed other archival observations with the source in the field of view to study its long-term variability.
Results. With the good position localisation capability of XMM-Newton, we identify the counterpart of the source as MQS J004241.66–734041.3, an active galactic nucleus (AGN) behind the SMC at a redshift of 0.95. The X-ray spectrum can be fitted with an absorbed power law with a photon-index of Γ = 1.7, which is consistent with that expected from AGNs. By comparing the current XMM-Newton observation with an archival XMM-Newton and two other ASCA observations of the source, we find signatures of long-term variability, another common phenomenon in AGNs. All of the above are consistent with AX J0043−737 being an AGN behind the SMC.
Genetic Variation in the von Willebrand Factor Gene in Swedish von Willebrand Disease Patients
Authors: Manderstedt Eric , Lind-Halldén Christina , Lethagen Stefan , Halldén Christer 
Published: January 2018

Abstractvon Willebrand factor (VWF) level and function are influenced by genetic variation in VWF and several other genes in von Willebrand disease type 1 (VWD1) patients. This study comprehensively screened for VWF variants and investigated the presence of ABO genotypes and common and rare VWF variants in Swedish VWD1 patients. The VWF gene was resequenced using Ion Torrent and Sanger sequencing in 126 index cases historically diagnosed with VWD. Exon 7 of the ABO gene was resequenced using Sanger sequencing. Multiplex ligation-dependent probe amplification analysis was used to investigate for copy number variants. Genotyping of 98 single nucleotide variants allowed allele frequency comparisons with public databases. Seven VWD2 mutations and 36 candidate VWD1 mutations (5 deletions, 4 nonsense, 21 missense, 1 splice, and 5 synonymous mutations) were identified. Nine mutations were found in more than one family and nine VWD1 index cases carried more than one candidate mutation. The T-allele of rs1063857 (c.2385T > C, p.Y795 = ) and blood group O were both frequent findings and contributed to disease in the Swedish VWD1 population. VWD2 mutations were found in 20 and candidate VWD1 mutations in 51 index cases out of 106 (48%). VWF mutations, a VWF haplotype, and blood group O all contributed to explain disease in Swedish VWD1 patients.
A Study of Coagulation Changes at Early Stage after Total Knee Arthroplasty
Authors: Huang Jian , Zhang Peng , Wang Dawei 
Published: January 2018

AbstractCoagulation changes after total knee arthroplasty (TKA) can provide evidence for optimization of timing of perioperative anticoagulation medicine. To evaluate the changes, 50 patients with knee osteoarthritis or rheumatoid arthritis were recruited for the study and underwent thrombelastography tests pre-, 6 hours, 12 hours, and 24 hours after TKA surgery and Doppler ultrasound examination 20 to 24 hours after surgery. The results have shown prolonged clotting time (R value) at 6 and 12 hours, while shortened at 24 hours. Similar results have been found in combined clotting and coagulation time (R + K value). However, other parameters, such as coagulation time (K value), coagulation speed (α angle), maximum amplitude (MA), and coagulation index (CI), have not significantly changed except that CI has increased significantly at 24 hours. Further Doppler ultrasound examination did not detect any deep venous thrombosis in all patients. The data showed that coagulation time after TKA within 12 hours, especially within the 6-hour time period, after surgery was significantly higher, while at 24 hours it became lower than the preoperation time. The results suggested that anticoagulation medicine is not necessary during the first 6 to 12 hours, but is required between 12 and 24 hours after surgery to reduce the risk of thrombosis.
Outcomes Following Low-Profile Mesh Plate Osteosynthesis of Patella Fractures
Authors: Siljander Matthew , Koueiter Denise , Gandhi Sapan , Wiater Brett , Wiater Patrick 
Published: January 2018

AbstractTreatment of patella fractures is fraught with complications and historically poor functional outcomes. A fixation method that allows for early mobilization and decreases symptomatic hardware rates will improve knee range of motion, postoperative functional status, and reoperation rates. The purpose of this study was to evaluate the functional outcomes after locked plate osteosynthesis of patella fractures at a Level 1 trauma center. A retrospective case series was conducted of patients who underwent open reduction internal fixation (ORIF) of a patella fracture using a locked mesh plating technique coupled with neutralization of forces on the distal pole of the patella. Twelve patients were evaluated at a mean follow-up of 19 months (range, 6–30) with physical exam, functional outcomes, and radiographs. There were 9 women and 3 men with an average age of 66.1 years (range, 53–75). Radiographic bony union was achieved in all patients by 3-month follow-up. Visual Analog Pain Score averaged 1.7 (median, 1.0; range, 0–8), the mean Knee Outcome Score – Activities of Daily Living Scale was 83.9 (median, 92.1; range, 45.7–100.0), the mean Short Form Musculoskeletal Function Assessment (SMFA) Function Index was 9.9 (median, 3.7; range, 0.7–41.2), and the mean SMFA Bother Index was 11.1 (median, 3.1; range, 0–62.5). The SF-36 Physical Component Score mean was 48.4 ± 8.5 and the SF-36 Mental Component Score mean was 54.1 ± 9.6. No complications developed and there were no reoperations for nonunion, infection, or symptomatic hardware. This study demonstrates that locked plate osteosynthesis for operative patella fractures can reliably achieve bony union with potentially superior functional outcomes as compared with traditional methods. Further studies are needed to evaluate plate fixation for patella fractures, but early results are promising.
Optimierung der Überleitung von der stationären in die ambulante Gesundheitsversorgung nach Schlaganfall (OpTheraS)
Authors: Saal Susanne , Kirchner-Heklau Uta , Müller Tobias , Wohlfarth Kai , Hamzei Farsin , Müller Melanie , Strobel Susanne , Clewing Carsten 
Published: January 2018

Zusammenfassung
Ziel Identifikation von Einflussfaktoren der Überleitung in die Häuslichkeit nach Schlaganfall und Ansätzen zur Prozessverbesserung.
Methodik Vergleich der Überleitung aus medizinischer Rehabilitation nach Hause gegenüber der Überleitung aus dem Akutkrankenhaus nach Hause durch Experteninterviews und Gruppendiskussionen mit Gesundheitsprofessionen, ein Scoping Review und eine standardisierte Patientenbefragung 6 Wochen nach Entlassung aus stationärer Behandlung per Telefon.
Ergebnisse Es wurden 14 Experten- und 3 Gruppeninterviews (n=18) geführt. Als Einflussfaktoren der Überleitung wurden u. a. Aspekte der Kommunikation, der sozialen Unterstützung und des Rollenverständnisses der Patienten identifiziert. Die standardisierte Patientenbefragung (n=110) zeigte eine Umsetzung von Empfehlungen fachärztlicher Konsultationen von 37% und von ambulanten Therapien bis zu 86%. Der Scoping Review identifizierte 7 systematische Übersichtsarbeiten, 21 randomisierte Studien und 5 kontrollierte Studien zu edukativen Maßnahmen, Information und Beratung, Early Supported Discharge, stroke liaison services, interprofessionellen Teamkonferenzen und Versorgungspfaden.
Schlussfolgerung Zur Verbesserung der Überleitung nach Hause ist es notwendig, über die Fallebene der Patienten hinaus, Abläufe innerhalb und zwischen den versorgenden Einrichtungen zu berücksichtigen. Die Entwicklung solcher Konzepte sollte sich methodisch am Vorgehen der Entwicklung und Evaluation komplexer Interventionen orientieren.
Polaronic behavior in a weak-coupling superconductor
Authors: Swartz Adrian G. , Inoue Hisashi , Merz Tyler A. , Hikita Yasuyuki , Raghu Srinivas , Devereaux Thomas P. , Johnston Steven , Hwang Harold Y. 
Published: January 2018

Significance
The perovskite oxide SrTiO3 is an n-type semiconductor, which exhibits superconductivity at low temperatures even though there are extraordinarily few electrons. In most materials, with so few electrons it becomes difficult to pair them into a macroscopic superconducting ground state. This regime is also unusual in that most theories of superconductivity rely on electrons that are much faster than their coupling (typically lattice vibrations or phonons). By using techniques in which tunneling junctions are engineered with atomic recision, we are able to probe the electronic structure of this material. The experiments indicate a large discrepancy between the electron–phonon coupling strength and the superconducting properties. This places SrTiO3, a host material for a variety of exotic quantum phases (i.e., in FeSe/SrTiO3, LaAlO3/SrTiO3), in a unique regime of superconductivity.
Effects of the ecto-ATPase apyrase on microglial ramification and surveillance reflect cell depolarization, not ATP depletion
Authors: Madry Christian , Arancibia-Cárcamo I. Lorena , Kyrargyri Vasiliki , Chan Victor T. T. , Hamilton Nicola B. , Attwell David 
Published: January 2018

SignificanceATP mediates interactions between cells in many tissues, but is particularly important for microglia, the brain’s immune cells, which constantly survey the brain to detect infection and regulate the brain’s wiring during development. It is controversial whether the ceaseless movement of microglia is driven by ATP release from brain cells. We show that an enzyme (apyrase) widely used to manipulate ATP levels is contaminated with K+ions which inhibit microglial surveillance, and that no ATP release is needed to drive microglial process movement. Thus, all conclusions about a role of ATP in signaling based on applying apyrase need reexamining, and brain immune surveillance is not regulated by ATP release.