bluuebunny/crossref_metadata_2025_split huggingface dataset:
Children's exposure to violent political conflict stimulates aggression at peers by increasing emotional distress, aggressive script rehearsal, and normative beliefs favoring aggression
Authors: Huesmann L. Rowell , Dubow Eric F. , Boxer Paul , Landau Simha F. , Gvirsman Shira Dvir , Shikaki Khalil 
Published: November 2016

AbstractWe examine the hypothesis that children's exposure to ethnic–political conflict and violence over the course of a year stimulates their increased aggression toward their own in-group peers in subsequent years. In addition, we examine what social cognitive and emotional processes mediate these effects and how these effects are moderated by gender, age, and ethnic group. To accomplish these aims, we collected three waves of data from 901 Israeli and 600 Palestinian youths (three age cohorts: 8, 11, and 14 years old) and their parents at 1-year intervals. Exposure to ethnic–political violence was correlated with aggression at in-group peers among all age cohorts. Using a cross-lagged structural equation model from Year 1 to Year 3, we found that the relation between exposure and aggression is more plausibly due to exposure to ethnic–political violence stimulating later aggression at peers than vice versa, and this effect was not moderated significantly by gender, age cohort, or ethnic group. Using three-wave structural equation models, we then showed that this effect was significantly mediated by changes in normative beliefs about aggression, aggressive script rehearsal, and emotional distress produced by the exposure. Again the best fitting model did not allow for moderation by gender, age cohort, or ethnic group. The findings are consistent with recent theorizing that exposure to violence leads to changes both in emotional processes promoting aggression and in the acquisition through observational learning of social cognitions promoting aggression.
Promoting children's learning and development in conflict-affected countries: Testing change process in the Democratic Republic of the Congo
Authors: Aber J. Lawrence , Tubbs Carly , Torrente Catalina , Halpin Peter F. , Johnston Brian , Starkey Leighann , Shivshanker Anjuli , Annan Jeannie , Seidman Edward , Wolf Sharon 
Published: November 2016

AbstractImproving children's learning and development in conflict-affected countries is critically important for breaking the intergenerational transmission of violence and poverty. Yet there is currently a stunning lack of rigorous evidence as towhetherandhowprograms to improve learning and development in conflict-affected countries actually work to bolster children's academic learning and socioemotional development. This study tests a theory of change derived from the fields of developmental psychopathology and social ecology about how a school-based universal socioemotional learning program, the International Rescue Committee's Learning to Read in a Healing Classroom (LRHC), impacts children's learning and development. The study was implemented in three conflict-affected provinces of the Democratic Republic of the Congo and employed a cluster-randomized waitlist control design to estimate impact. Using multilevel structural equation modeling techniques, we found support for the central pathways in the LRHC theory of change. Specifically, we found that LRHC differentially impacted dimensions of the quality of the school and classroom environment at the end of the first year of the intervention, and that in turn these dimensions of quality were differentially associated with child academic and socioemotional outcomes. Future implications and directions are discussed.
Building a translational science on children and youth affected by political violence and armed conflict: A commentary
Authors: Masten Ann S. 
Published: November 2016

AbstractArticles in this timely Special Section represent an important milestone in the developmental science on children and youth involved in political violence and armed conflict. With millions of children worldwide affected by past and present wars and conflicts, there is an urgent and growing need for research to inform efforts to understand, prevent, and mitigate the possible harm of such violence to individual children, families, communities, and societies, for present as well as future generations. The four programs of research highlighted in this Special Section illustrate key advances and challenges in contemporary development research on young people growing up in the midst or aftermath of political violence. These studies are longitudinal, methodologically sophisticated, and grounded in socioecological systems models that align well with current models of risk and resilience in developmental psychopathology. These studies collectively mark a critically important shift to process-focused research that holds great promise for translational applications. Nonetheless, given the scope of the international crisis of children and youth affected by political violence and its sequelae, there is an urgent global need for greater mobilization of resources to support translational science and effective evidence-based action.
Conducting longitudinal, process-oriented research with conflict-affected youth: Solving the inevitable challenges
Authors: Dubow Eric F. , Aber J. Lawrence , Betancourt Theresa S. , Cummings E. Mark , Huesmann L. Rowell 
Published: November 2016

AbstractThe reader might get the impression that the four projects described in this Special Section proceeded in a systematic and predictable way. Of course, those of us engaged in each research project encountered pitfalls and challenges along the way. A main goal of this Special Section is to provide pathways and encouragement for those who may be interested in advancing high-quality research on this topic. In this paper, we describe a set of practical and ethical challenges that we encountered in conducting our longitudinal, process-oriented, and translational research with conflict-affected youth, and we illustrate how problems can be solved with the goal of maintaining the internal and external validity of the research designs. We are hopeful that by describing the challenges of our work, and how we overcame them, which are seldom treated in this or any other literature on research on child development in high-risk contexts, we can offer a realistic and encouraging picture of conducting methodologically sound research in conflict-affected contexts.
Wolff–Parkinson–White syndrome: a single exercise stress test might be misleading
Authors: Salavitabar Arash , Silver Eric S. , Liberman Leonardo 
Published: November 2016

AbstractRisk stratification of patients with Wolff–Parkinson–White syndrome for sudden death is a complex process, particularly in understanding the utility of the repeat exercise stress test. We report a case of an 18-year-old patient who was found to have a high-risk pathway by both invasive and exercise stress testing after an initial exercise stress test showing beat-to-beat loss of pre-excitation.
Percutaneous closure of an aortopulmonary window using Amplatzer Duct Occluder II: Additional Sizes: the first reported case
Authors: Fiszer Roland , Zbroński Karol , Szkutnik Małgorzata 
Published: November 2016

AbstractTo date, there are no reported cases of the Amplatzer Duct Occluder II: Additional Sizes’ use in percutaneous closure of an aortopulmonary window. We report a case of percutaneous closure of an aortopulmonary window in a 4.5-month-old, 6 kg child. Owing to the patient’s low weight, high risk of damage to the pulmonary valve, as well as the possibility of aortic and pulmonary artery obstruction, classic implants were deemed unsuitable and a decision was made to use the Amplatzer Duct Occluder II: Additional Sizes. The implant performed very well – the soft waist filled the aortopulmonary connection and both retention discs were properly shaped and pressed against vessel walls. Echocardiogram performed 12 hours after the procedure confirmed a correct occluder position. Low-profile retention discs had no impact on pulmonary valve function, despite the defect’s proximity to the valve. No obstruction of the aortic or pulmonary artery lumen was noted. The Amplatzer Duct Occluder II: Additional Size implant is a safe and useful device for percutaneous closure of an aortopulmonary window in a carefully selected group of patients.
Oxytocin Reduces Face Processing Time but Leaves Recognition Accuracy and Eye-Gaze Unaffected
Authors: Hubble Kelly , Daughters Katie , Manstead Antony S.R. , Rees Aled , Thapar Anita , van Goozen Stephanie H.M. 
Published: November 2016

AbstractObjectives: Previous studies have found that oxytocin (OXT) can improve the recognition of emotional facial expressions; it has been proposed that this effect is mediated by an increase in attention to the eye-region of faces. Nevertheless, evidence in support of this claim is inconsistent, and few studies have directly tested the effect of oxytocin on emotion recognition via altered eye-gaze Methods: In a double-blind, within-subjects, randomized control experiment, 40 healthy male participants received 24 IU intranasal OXT and placebo in two identical experimental sessions separated by a 2-week interval. Visual attention to the eye-region was assessed on both occasions while participants completed a static facial emotion recognition task using medium intensity facial expressions. Results: Although OXT had no effect on emotion recognition accuracy, recognition performance was improved because face processing was faster across emotions under the influence of OXT. This effect was marginally significant (p<.06). Consistent with a previous study using dynamic stimuli, OXT had no effect on eye-gaze patterns when viewing static emotional faces and this was not related to recognition accuracy or face processing time. Conclusions: These findings suggest that OXT-induced enhanced facial emotion recognition is not necessarily mediated by an increase in attention to the eye-region of faces, as previously assumed. We discuss several methodological issues which may explain discrepant findings and suggest the effect of OXT on visual attention may differ depending on task requirements. (JINS, 2017, 23, 23–33)
Site-Specific Preparation of Intact Solid–Liquid Interfaces by Label-FreeIn SituLocalization and Cryo-Focused Ion Beam Lift-Out
Authors: Zachman Michael J. , Asenath-Smith Emily , Estroff Lara A. , Kourkoutis Lena F. 
Published: November 2016

AbstractScanning transmission electron microscopy (STEM) allows atomic scale characterization of solid–solid interfaces, but has seen limited applications to solid–liquid interfaces due to the volatility of liquids in the microscope vacuum. Although cryo-electron microscopy is routinely used to characterize hydrated samples stabilized by rapid freezing, sample thinning is required to access the internal interfaces of thicker specimens. Here, we adapt cryo-focused ion beam (FIB) “lift-out,” a technique recently developed for biological specimens, to prepare intact internal solid–liquid interfaces for high-resolution structural and chemical analysis by cryo-STEM. To guide the milling process we introduce a label-freein situmethod of localizing subsurface structures in suitable materials by energy dispersive X-ray spectroscopy (EDX). Monte Carlo simulations are performed to evaluate the depth-probing capability of the technique, and show good qualitative agreement with experiment. We also detail procedures to produce homogeneously thin lamellae, which enable nanoscale structural, elemental, and chemical analysis of intact solid–liquid interfaces by analytical cryo-STEM. This work demonstrates the potential of cryo-FIB lift-out and cryo-STEM for understanding physical and chemical processes at solid–liquid interfaces.
Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)
Authors: Golpour Amin , Pšenička Martin , Niksirat Hamid 
Published: November 2016

AbstractCalcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet,Acipenser ruthenus, using a combined oxalate–pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.
CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT
Authors: Chaudhury Arindam , Cheema Shebna , Fachini Joseph M. , Kongchan Natee , Lu Guojun , Simon Lukas M. , Wang Tao , Mao Sufeng , Rosen Daniel G. , Ittmann Michael M. , Hilsenbeck Susan G. , Shaw Chad A. , Neilson Joel R. 
Published: November 2016

AbstractThe importance of translational regulation in tumour biology is increasingly appreciated. Here, we leverage polyribosomal profiling to prospectively define translational regulatory programs underlying epithelial-to-mesenchymal transition (EMT) in breast epithelial cells. We identify a group of ten translationally regulated drivers of EMT sharing a common GU-rich cis-element within the 3′-untranslated region (3′-UTR) of their mRNA. These cis-elements, necessary for the regulatory activity imparted by these 3′-UTRs, are directly bound by the CELF1 protein, which itself is regulated post-translationally during the EMT program. CELF1 is necessary and sufficient for both mesenchymal transition and metastatic colonization, and CELF1 protein, but not mRNA, is significantly overexpressed in human breast cancer tissues. Our data present an 11-component genetic pathway, invisible to transcriptional profiling approaches, in which the CELF1 protein functions as a central node controlling translational activation of genes driving EMT and ultimately tumour progression.
Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
Authors: Cheng Hui , Ang Heather Yin-Kuan , A. EL Farran Chadi , Li Pin , Fang Hai Tong , Liu Tong Ming , Kong Say Li , Chin Michael Lingzi , Ling Wei Yin , Lim Edwin Kok Hao , Li Hu , Huber Tara , Loh Kyle M. , Loh Yuin-Han , Lim Bing 
Published: November 2016

AbstractRecent efforts have attempted to convert non-blood cells into hematopoietic stem cells (HSCs) with the goal of generating blood lineages de novo. Here we show that hematopoietic transcription factors Scl, Lmo2, Runx1 and Bmi1 can convert a developmentally distant lineage (fibroblasts) into ‘induced hematopoietic progenitors’ (iHPs). Functionally, iHPs generate acetylcholinesterase+ megakaryocytes and phagocytic myeloid cells in vitro and can also engraft immunodeficient mice, generating myeloerythoid and B-lymphoid cells for up to 4 months in vivo. Molecularly, iHPs transcriptionally resemble native Kit+ hematopoietic progenitors. Mechanistically, reprogramming factor Lmo2 implements a hematopoietic programme in fibroblasts by rapidly binding to and upregulating the Hhex and Gfi1 genes within days. Moreover the reprogramming transcription factors also require extracellular BMP and MEK signalling to cooperatively effectuate reprogramming. Thus, the transcription factors that orchestrate embryonic hematopoiesis can artificially reconstitute this programme in developmentally distant fibroblasts, converting them into engraftable blood progenitors.
Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells
Authors: Kraehling Jan R. , Chidlow John H. , Rajagopal Chitra , Sugiyama Michael G. , Fowler Joseph W. , Lee Monica Y. , Zhang Xinbo , Ramírez Cristina M. , Park Eon Joo , Tao Bo , Chen Keyang , Kuruvilla Leena , Larriveé Bruno , Folta-Stogniew Ewa , Ola Roxana , Rotllan Noemi , Zhou Wenping , Nagle Michael W. , Herz Joachim , Williams Kevin Jon , Eichmann Anne , Lee Warren L. , Fernández-Hernando Carlos , Sessa William C. 
Published: November 2016

AbstractIn humans and animals lacking functional LDL receptor (LDLR), LDL from plasma still readily traverses the endothelium. To identify the pathways of LDL uptake, a genome-wide RNAi screen was performed in endothelial cells and cross-referenced with GWAS-data sets. Here we show that the activin-like kinase 1 (ALK1) mediates LDL uptake into endothelial cells. ALK1 binds LDL with lower affinity than LDLR and saturates only at hypercholesterolemic concentrations. ALK1 mediates uptake of LDL into endothelial cells via an unusual endocytic pathway that diverts the ligand from lysosomal degradation and promotes LDL transcytosis. The endothelium-specific genetic ablation of Alk1 in Ldlr-KO animals leads to less LDL uptake into the aortic endothelium, showing its physiological role in endothelial lipoprotein metabolism. In summary, identification of pathways mediating LDLR-independent uptake of LDL may provide unique opportunities to block the initiation of LDL accumulation in the vessel wall or augment hepatic LDLR-dependent clearance of LDL.
Classic IL-6R signalling is dispensable for intestinal epithelial proliferation and repair
Authors: Aden K , Breuer A , Rehman A , Geese H , Tran F , Sommer J , Waetzig G H , Reinheimer T M , Schreiber S , Rose-John S , Scheller J , Rosenstiel P 
Published: November 2016

AbstractInflammatory bowel disease is characterized by disturbed cytokine signalling in the mucosa. Inhibition of the proinflammatory interleukin (IL)-6 pathway is a promising new therapeutic strategy, but safety concerns arise as IL-6 signalling also contributes to epithelial repair of the intestinal mucosa. To which extent IL-6 classic or trans-signalling contributes to intestinal repair remains elusive. We tested the influence of IL-6 classic signalling on intestinal repair and proliferation. Whereas IL-6 induced STAT3 phosphorylation in the colonic cancer cell lines, primary non-malignant intestinal organoids did not respond to IL-6 classic signalling. Mice deficient in intestinal IL-6R (IL-6RΔIEC mice) did not display increased susceptibility to acute dextran sulfate sodium (DSS)-induced colitis. In the azoxymethane DSS model IL-6RΔIEC mice were not protected from inflammation-induced carcinogenesis but showed comparable tumor load to wild-type mice. These data indicate that classic signalling is not the major pathway to transduce IL-6 stimuli into the intestinal epithelium.
N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma
Authors: Li Jiang-Hua , Huang Wan , Lin Peng , Wu Bo , Fu Zhi-Guang , Shen Hao-Miao , Jing Lin , Liu Zhen-Yu , Zhou Yang , Meng Yao , Xu Bao-Qing , Chen Zhi-Nan , Jiang Jian-Li 
Published: November 2016

AbstractCluster of differentiation 147 (CD147), also known as extracellular matrix metalloproteinase inducer, is a transmembrane glycoprotein that mediates oncogenic processes partly through N-glycosylation modifications. N-glycosylation has been demonstrated to be instrumental for the regulation of CD147 function during malignant transformation. However, the role that site-specific glycosylation of CD147 plays in its defective function in hepatocellular carcinomacells needs to be determined. Here, we demonstrate that the modification of N-glycosylation at Asn152 on CD147 strongly promotes hepatocellular carcinoma (HCC) invasion and migration. After the removal of N-glycans at Asn152, CD147 was more susceptible to degradation by ER-localized ubiquitin ligase-mediated endoplasmic reticulum-associated degradation (ERAD). Furthermore, N-linked glycans at Asn152 were required for CD147 to acquire and maintain proper folding in the ER. Moreover, N-linked glycans at Asn152 functioned as a recognition motif that was directly mediated by the CNX quality control system. Two phases in the retention-based ER chaperones system drove ER-localized CD147 trafficking to degradation. Deletion of N-linked glycosylation at Asn152 on CD147 significantly suppressed in situ tumour metastasis. These data could potentially shed light on the molecular regulation of CD147 through glycosylation and provide a valuable means of developing drugs that target N-glycans at Asn152 on CD147.
Using the virtual reality device Oculus Rift for neuropsychological assessment of visual processing capabilities
Authors: Foerster Rebecca M. , Poth Christian H. , Behler Christian , Botsch Mario , Schneider Werner X. 
Published: November 2016

AbstractNeuropsychological assessment of human visual processing capabilities strongly depends on visual testing conditions including room lighting, stimuli, and viewing-distance. This limits standardization, threatens reliability, and prevents the assessment of core visual functions such as visual processing speed. Increasingly available virtual reality devices allow to address these problems. One such device is the portable, light-weight, and easy-to-use Oculus Rift. It is head-mounted and covers the entire visual field, thereby shielding and standardizing the visual stimulation. A fundamental prerequisite to use Oculus Rift for neuropsychological assessment is sufficient test-retest reliability. Here, we compare the test-retest reliabilities of Bundesen’s visual processing components (visual processing speed, threshold of conscious perception, capacity of visual working memory) as measured with Oculus Rift and a standard CRT computer screen. Our results show that Oculus Rift allows to measure the processing components as reliably as the standard CRT. This means that Oculus Rift is applicable for standardized and reliable assessment and diagnosis of elementary cognitive functions in laboratory and clinical settings. Oculus Rift thus provides the opportunity to compare visual processing components between individuals and institutions and to establish statistical norm distributions.
Sub-optimality in motor planning is retained throughout 9 days practice of 2250 trials
Authors: Ota Keiji , Shinya Masahiro , Kudo Kazutoshi 
Published: November 2016

AbstractOptimality in motor planning, as well as accuracy in motor execution, is required to maximize expected gain under risk. In this study, we tested whether humans are able to update their motor planning. Participants performed a coincident timing task with an asymmetric gain function, in which optimal response timing to gain the highest total score depends on response variability. Their behaviours were then compared using a Bayesian optimal decision model. After 9 days of practicing 2250 trials, the total score increased, and temporal variance decreased. On the other hand, the participants showed consistent risk-seeking or risk-averse behaviour, preserving suboptimal motor planning. These results suggest that a human’s computational ability to calculate an optimal motor plan is limited, and it is difficult to improve it through repeated practice with a score feedback.