bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in cannabis potency and first-time admissions to drug treatment: a 16-year study in the Netherlands
Authors: Freeman Tom P. , van der Pol Peggy , Kuijpers Wil , Wisselink Jeroen , Das Ravi K. , Rigter Sander , van Laar Margriet , Griffiths Paul , Swift Wendy , Niesink Raymond , Lynskey Michael T. 
Published: January 2018

AbstractBackgroundThe number of people entering specialist drug treatment for cannabis problems has increased considerably in recent years. The reasons for this are unclear, but rising cannabis potency could be a contributing factor.MethodsCannabis potency data were obtained from an ongoing monitoring programme in the Netherlands. We analysed concentrations of δ-9-tetrahydrocannabinol (THC) from the most popular variety of domestic herbal cannabis sold in each retail outlet (2000–2015). Mixed effects linear regression models examined time-dependent associations between THC and first-time cannabis admissions to specialist drug treatment. Candidate time lags were 0–10 years, based on normative European drug treatment data.ResultsTHC increased from a mean (95% CI) of 8.62 (7.97–9.27) to 20.38 (19.09–21.67) from 2000 to 2004 and then decreased to 15.31 (14.24–16.38) in 2015. First-time cannabis admissions (per 100 000 inhabitants) rose from 7.08 to 26.36 from 2000 to 2010, and then decreased to 19.82 in 2015. THC was positively associated with treatment entry at lags of 0–9 years, with the strongest association at 5 years, b = 0.370 (0.317–0.424), p < 0.0001. After adjusting for age, sex and non-cannabis drug treatment admissions, these positive associations were attenuated but remained statistically significant at lags of 5–7 years and were again strongest at 5 years, b = 0.082 (0.052–0.111), p < 0.0001.ConclusionsIn this 16-year observational study, we found positive time-dependent associations between changes in cannabis potency and first-time cannabis admissions to drug treatment. These associations are biologically plausible, but their strength after adjustment suggests that other factors are also important.
Three-dimensional nonlinear force-sensing method based on double microgrippers with E-type vertical elastomer for minimally invasive robotic surgery
Authors: Yu Lingtao , Yan Yusheng , Li Chenzheng , Zhang Xiufeng 
Published: January 2018

SUMMARYThis paper presents a new type of forceps that consist of two microgrippers with the capability of direct force sensing, which enables grasping and manipulating forces at the tip of surgical instrument for minimally invasive robotic surgery. For the prototype design of the forceps, a double E-type vertical elastomer with four strain beams is presented, whose force-sensing principle is expounded. Thus, the forceps with the elastomer can be considered a compliant component, which provides tiny displacements that allow large strain, and the overall diameter is 10 mm. The sizes of the elastomer and forceps are successively determined by analyzing the relationship of several parameters and strain. Then, the linearity analysis of strain beams determines the positions to apply gauges for sensing. The two-dimensional force decoupling models for a single microgripper are proposed based on piecewise analytical polynomials of the strain difference and employed to develop a new three-dimensional force nonlinear decoupling algorithm based on double microgrippers, which realizes single-axial grasping and three-axial pulling forces. Finally, the required force-sensing performance of the proposed method is successfully verified in theory using finite-element simulations.
Characterization of a low-grade copper-sulphide ore to assess its suitability for in situ recovery
Authors: Li Jian , Kuhar Laura , Austin Peter , Da Costa Micheal 
Published: January 2018

CSIRO Minerals is developing new technologies and approaches for the in situ recovery (ISR) of valuable metals. ISR provides opportunities to process small and/or deep deposits and could create additional revenue for conventional uneconomic mines. Unlike for conventional processing, no standard methodology exists for characterizing sample suitability for ISR. The authors are developing a workflow to understand sample and deposit amenability to ISR processing. A South Australian low-grade iron-oxide-copper-gold sulphide ore was studied. A total of 37 drill cores samples were obtained from the ore sample for detailed mineralogical and leaching characterization. A range of characterization techniques including chemical analysis, X-ray fluorescence mapping, quantitative evaluation of minerals by scanning electron microscopy, computed tomography scanning, and bulk- and micro-X-ray diffraction analyses were applied to understand the ore mineralogy. Leaching tests at 50 °C were performed on selected samples to understanding their leaching behaviour. Mineralogical characterization found that copper was present mostly as discrete secondary copper sulphides distributed in various areas and at various specimen depths, which, in theory should be readily available for leaching, provided sufficient solution access exists. Leaching results showed steady copper recovery under the conditions tested. This study provides insight into understanding the suitability of an ore for ISR processing.
Spatial variability of elements in ancient Greek (ca. 600–250 BC) silver coins using scanning electron microscopy with energy dispersive spectrometry (SEM-EDS) and time of flight-secondary ion mass spectrometry (ToF-SIMS)
Authors: Marjo Christopher E. , Davis Gillan , Gong Bin , Gore Damian B. 
Published: January 2018

Archaeometrists use a variety of analytical methods to determine trace elements in ancient Greek silver coins, for provenance studies, understanding social and technological change, and authentication. One analytical problem which is little documented is understanding the horizontal spatial heterogeneity of coin elemental composition in micro-sampled areas, which are usually assumed to be uniform. This study analysed ten ancient Greek coins representative of silver circulating in the Aegean region in the sixth to third centuries BC. Scanning electron microscopy with energy dispersive spectrometry was used to map the spatial distribution of elements on coins that were abraded to remove the patina. Time of flight-secondary ion mass spectrometry was then conducted on selected coins, mapping an area ~100 × 100 µm and depth profiling from 0 to 10 µm. These data revealed the three-dimensional elemental complexity of the coins, in particular, the heterogeneity both in the patina and beneath it. These data will guide future authentication and provenance studies of larger sample sets of ancient Greek coins including the use of line scanning for laser ablation inductively coupled plasma mass spectrometry data collection rather than spot analyses, and non-destructive analytical techniques such as X-ray fluorescence spectrometry.
Understanding Multiple Perspectives: The Contribution of a Short-Essay Assignment to Teaching about the Arab–Israeli Conflict
Authors: Yakter Alon , Tessler Mark 
Published: January 2018

ABSTRACTTeaching about political conflict requires an understanding of the multiple perspectives and motivations of the parties to the conflict. This is especially true when teaching students who may have strong predispositions, such as in classes on the Arab–Israeli conflict. Various active-learning techniques address this problem but often at a cost of long preparation, substantial class time, and class-size limitations. This article offers both quantitative and qualitative evidence that a short essay asking students to defend a key action taken by one of the actors makes them more understanding and less accusatory of that side—even as it does not change their overall attitude toward the conflict. Adding a small-group discussion and a written reflection further helps students to make more informed and reasoned judgments. Importantly, such an assignment is easy to create, implement, and modify across various class types, sizes, and constraints.
Spatiotemporal heterogeneity of malnutrition indicators in children under 5 years of age in Bangladesh, 1999–2011
Authors: Hasan Md Tanvir , Mamun Abdullah A , Williams Gail M , Soares Magalhães Ricardo J 
Published: January 2018

AbstractObjectiveTo examine changes in the spatial clustering of malnutrition in children under 5 years of age (under-5s) for the period 1999 to 2011 in Bangladesh.DesignWe used data from four nationally representative Demographic and Health Surveys (DHS) conducted in 1999–2000, 2004, 2007 and 2011 in Bangladesh involving a total of 24 211 under-5s located in 1661 primary sampling units (PSU; geographical unit of analysis) throughout Bangladesh. The prevalence of stunting (height/length-for-age Z-score <−2), underweight (weight-for-age Z-score <−2) and wasting (weight-for-height/length Z-score <−2) at each PSU site and for each survey year were estimated based on the WHO child growth standard. The extent of spatial clustering was quantified using semivariograms.SettingWhole of Bangladesh.SubjectsChildren under 5 years of age.ResultsOur results demonstrate that in 1999–2000 most PSU throughout Bangladesh experienced stunting, underweight and wasting prevalence which exceeded the WHO thresholds. By 2011, this situation improved, although in two of the six divisions (Barisal and Sylhet) PSU still exhibited higher levels of malnutrition compared with other divisions of the country. The pattern of spatial clustering for stunting, underweight and wasting also changed between 1999 and 2011 both at national and sub-national (division) levels.ConclusionsWe identified divisions where malnutrition indicators (stunting, underweight and wasting) remain highly clustered and other divisions where they are more widely spread in Bangladesh. This has important implications on how interventions for malnutrition need to be delivered (geographically targeted interventions v. random interventions) within each division of the country.
Attitudes towards the scientific search for extraterrestrial life among Swedish high school and university students
Authors: Persson Erik , Capova Klara Anna , Li Yuan 
Published: January 2018

AbstractThe aim of this study is to increase our understanding of the attitudes towards the scientific search for extraterrestrial life among high school and university students in Sweden. The most important results of the analysis are that: (a) the great majority of students believe that extraterrestrial life exists; (b) most students regard searching for extraterrestrial life to be quite important or very important; (c) very few students think that we should actively avoid searching for extraterrestrial life; (d) the most common motive for assigning a high priority to search for extraterrestrial life is that it is interesting, the most common motive for assigning a low priority is that such knowledge would not be practically useful, or that the money would be better spent elsewhere; (e) most students do not think they are very well informed regarding the search for extraterrestrial life. A higher percentage of the students who judge themselves to be well informed also believe that extraterrestrial life exists. We have also found some differences between subgroups (men/women, high school students/university students and different fields of study), but the differences are with few exceptions small in comparison with the overall trends, and they mostly differ in degree rather than direction.
Social affective context reveals altered network dynamics in schizophrenia patients
Authors: Hendler Talma , Raz Gal , Shimrit Solnik , Jacob Yael , Lin Tamar , Roseman Leor , Wahid Wahid Madah , Kremer Ilana , Kupchik Marina , Kotler Moshe , Bleich-Cohen Maya 
Published: January 2018

AbstractImpairments in social cognition and interactions are core psychopathologies in schizophrenia, often manifesting as an inability to appropriately relate to the intentions and feelings of others. Neuroimaging has helped to demarcate the dynamics of two distinct functional connectivity circuits underlying the social-affective processes related to mentalization (known as Theory of Mind, ToM) and somatic-affiliation (known as Embodied Simulation, ES). While evidence points to abnormal activation patterns within these networks among those suffering from schizophrenia, it is yet unclear however, if these patients exhibit this abnormal functional connectivity in the context of social-affective experiences. The current fMRI study, investigated functional connectivity dynamics within ToM and ES networks as subjects experienced evolving cinematic portrayals of fear. During scanning, schizophrenia patients and healthy controls passively watched a cinematic scene in which a mother and her son face various threatening events. Participants then provided a continuous and retrospective report of their fear intensity during a second viewing outside the scanner. Using network cohesion index (NCI) analysis, we examined modulations of ES-related and ToM-related functional connectivity dynamics and their relation to symptom severity and the continuous emotional ratings of the induced cinematic fear. Compared to patients, healthy controls showed higher ES-NCI and marginally lower ToM-NCI during emotional peaks. Cross-correlation analysis revealed an intriguing dynamic between NCI and the inter-group difference of reported fear. Schizophrenia patients rated their fear aslowerrelative to healthy controls, shortly after exhibitinglowerES connectivity. This increased difference in rating was also followed byhigherToM connectivity among schizophrenia patients. The clinical relevance of these findings is further highlighted by the following two results: (a) ToM-NCI was found to have a strong correlation with the severity of general symptoms during one of the two main emotional peaks (SpearmanR = 0.77); and (b)k-mean clustering demonstrated that the networks’ NCI dynamic during the social-affective context reliably differentiated between patients and controls. Together, these findings point to a possible neural marker for abnormal social-affective processing in schizophrenia, manifested as the disturbed balance between two functional networks involved in social-affective affiliation. This in turn suggests that exaggerated mentalization over somatic-affiliative processing, in response to another’s’ distress may underlie social-affective deficits in schizophrenia.
Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
Authors: Neggers Jasper Edgar , Kwanten Bert , Dierckx Tim , Noguchi Hiroki , Voet Arnout , Bral Lotte , Minner Kristien , Massant Bob , Kint Nicolas , Delforge Michel , Vercruysse Thomas , Baloglu Erkan , Senapedis William , Jacquemyn Maarten , Daelemans Dirk 
Published: January 2018

AbstractUnraveling the mechanism of action and molecular target of small molecules remains a major challenge in drug discovery. While many cancer drugs target genetic vulnerabilities, loss-of-function screens fail to identify essential genes in drug mechanism of action. Here, we report CRISPRres, a CRISPR-Cas-based genetic screening approach to rapidly derive and identify drug resistance mutations in essential genes. It exploits the local genetic variation created by CRISPR-Cas-induced non-homologous end-joining (NHEJ) repair to generate a wide variety of functional in-frame mutations. Using large sgRNA tiling libraries and known drug–target pairs, we validate it as a target identification approach. We apply CRISPRres to the anticancer agent KPT-9274 and identify nicotinamide phosphoribosyltransferase (NAMPT) as its main target. These results present a powerful and simple genetic approach to create many protein variants that, in combination with positive selection, can be applied to reveal the cellular target of small-molecule inhibitors.
Cell fate in antiviral response arises in the crosstalk of IRF, NF-κB and JAK/STAT pathways
Authors: Czerkies Maciej , Korwek Zbigniew , Prus Wiktor , Kochańczyk Marek , Jaruszewicz-Błońska Joanna , Tudelska Karolina , Błoński Sławomir , Kimmel Marek , Brasier Allan R. , Lipniacki Tomasz 
Published: January 2018

AbstractThe innate immune system processes pathogen-induced signals into cell fate decisions. How information is turned to decision remains unknown. By combining stochastic mathematical modelling and experimentation, we demonstrate that feedback interactions between the IRF3, NF-κB and STAT pathways lead to switch-like responses to a viral analogue, poly(I:C), in contrast to pulse-like responses to bacterial LPS. Poly(I:C) activates both IRF3 and NF-κB, a requirement for induction of IFNβ expression. Autocrine IFNβ initiates a JAK/STAT-mediated positive-feedback stabilising nuclear IRF3 and NF-κB in first responder cells. Paracrine IFNβ, in turn, sensitises second responder cells through a JAK/STAT-mediated positive feedforward pathway that upregulates the positive-feedback components: RIG-I, PKR and OAS1A. In these sensitised cells, the ‘live-or-die’ decision phase following poly(I:C) exposure is shorter—they rapidly produce antiviral responses and commit to apoptosis. The interlinked positive feedback and feedforward signalling is key for coordinating cell fate decisions in cellular populations restricting pathogen spread.
A NIN-LIKE PROTEIN mediates nitrate-induced control of root nodule symbiosis in Lotus japonicus
Authors: Nishida Hanna , Tanaka Sachiko , Handa Yoshihiro , Ito Momoyo , Sakamoto Yuki , Matsunaga Sachihiro , Betsuyaku Shigeyuki , Miura Kenji , Soyano Takashi , Kawaguchi Masayoshi , Suzaki Takuya 
Published: January 2018

AbstractLegumes and rhizobia establish symbiosis in root nodules. To balance the gains and costs associated with the symbiosis, plants have developed two strategies for adapting to nitrogen availability in the soil: plants can regulate nodule number and/or stop the development or function of nodules. Although the former is accounted for by autoregulation of nodulation, a form of systemic long-range signaling, the latter strategy remains largely enigmatic. Here, we show that the Lotus japonicus NITRATE UNRESPONSIVE SYMBIOSIS 1 (NRSYM1) gene encoding a NIN-LIKE PROTEIN transcription factor acts as a key regulator in the nitrate-induced pleiotropic control of root nodule symbiosis. NRSYM1 accumulates in the nucleus in response to nitrate and directly regulates the production of CLE-RS2, a root-derived mobile peptide that acts as a negative regulator of nodule number. Our data provide the genetic basis for how plants respond to the nitrogen environment and control symbiosis to achieve proper plant growth.
Lateral epitaxial heterojunctions in single nanowires fabricated by masked cation exchange
Authors: Dogan Sedat , Kudera Stefan , Dang Zhiya , Palazon Francisco , Petralanda Urko , Artyukhin Sergey , De Trizio Luca , Manna Liberato , Krahne Roman 
Published: January 2018

AbstractCation exchange is a versatile tool to control the composition of nanocrystals, and recently deterministic patterning could be achieved by combining it with lithography techniques. Regarding single nanocrystal structures, such spatial control of cation exchange enables the design of heterostructures, which can be integrated in functional optoelectronic elements. In this work, we fabricate nanowire CdSe/Cu2Se heterojunctions by masking cation exchange via electron-beam irradiation, such that cation exchange proceeds only in the non-irradiated sections. Interestingly, the heterojunction interfaces are almost atomically sharp, and the adjacent CdSe and Cu2Se domains exhibit epitaxial relationships. We show that the cation exchange at the CdSe/Cu2Se interface is only possible if the displaced Cd2+ ions can radially out-diffuse to the solution phase. If this exit pathway is blocked, the cation exchange cannot occur. Our technique allows one to transform already contacted single nanowires, and the obtained heterojunction nanowires manifest a noticeable gain in conductance.
Matched asymptotic solution for crease nucleation in soft solids
Authors: Ciarletta P. 
Published: January 2018

AbstractA soft solid subjected to a large compression develops sharp self-contacting folds at its free surface, known as creases. Creasing is physically different from structural elastic instabilities, like buckling or wrinkling. Indeed, it is a fully nonlinear material instability, similar to a phase-transformation. This work provides theoretical insights of the physics behind crease nucleation. Creasing is proved to occur after a global bifurcation allowing the co-existence of an outer deformation and an inner solution with localised self-contact at the free surface. The most fundamental result here is the analytic prediction of the nucleation threshold, in excellent agreement with experiments and numerical simulations. A matched asymptotic solution is given within the intermediate region between the two co-existing states. The self-contact acts like the point-wise disturbance in the Oseen’s correction for the Stokes flow past a circle. Analytic expressions of the matching solution and its range of validity are also derived.
Methods for Sterilizing Clinically Relevant Wear Particles Isolated from Metal-on-Metal Hip Implants
Authors: Fung Ernest S. , Unice Kenneth M. , Paustenbach Dennis J. , Finley Brent L. , Kovochich Michael 
Published: January 2018

AbstractEngineered or incidental particles may contain endotoxin from contaminated environments associated with generation, production, or handling activities. Endotoxins are ubiquitous contaminants that may yield false positive responses in immunological assays if present. The purpose of this study was to develop a sterilization method for removal of endotoxin from clinically relevant wear particles isolated from metal-on-metal (MoM) hip implant lubricant. In this case, the goal of particle sterilization was to sufficiently reduce endotoxin levels to acceptable levels for sensitive biological assays while retaining the physical and chemical characteristics of the original particles. Optimization of treatment with 0.05 NaOH in 50% ethanol successfully achieved a 5-log (>99.999%) reduction of endotoxin content while retaining the size and chemistry of MoM hip implant wear particles. Using the optimized method, the concentration of endotoxin was reduced from 161,000 to 1.19 EU/mL. As particle types can vary, sterilization strategies will also differ to optimize endotoxin removal while retaining key particle characteristics. To our knowledge, this study represents the first published sterilization method for clinically relevant MoM hip implant wear particles isolated from serum-rich lubricant.