bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Four In Situ Hybridization Methods, Based on Digoxigenin- and Biotin-Labelled Probes, in Detecting HPV DNA in Male Condylomata Acuminata
Authors: Lassus J , Niemi K-M , Marjamäki A , Syrjanen S , Kartamaa M , Lehmus A , Krohn K , Ranki A 
Published: January 2017

We have compared the efficacy of digoxigenin- and biotin-labelled probes in detecting HPV DNA by in situ hybridization on paraffin-embedded tissue sections of 57 male condyloma-suspect genital lesions. Each biopsy was hybridized with at least three of the following four methods: digoxigenin-labelled HPV DNA probes (Dig-HPV), biotinylated HPV-DNA probes (Bio-HPV), and two commercial methods (ViraType in situ and PathoGene), both based on biotinylated DNA probes. The hybridization products were visualized with colourigenic enzyme substrates. In most biopsies, the 4 methods gave equal results although cross-hybridization was most often found with the low-stringency ViraType method. Dig-HPV 6/11 probes gave positive results about twice as often as either of the commercial methods. No such difference, however, was found for HPV 16/18 probes. DNA of any type of HPV 6/11, 16/18 or 31/33/35 or 51 was detected in 28/43 (65%) of lesions showing condyloma acuminatum histology but in none of the 14 biopsies with no histological signs of HPV infection. In HPV-positive condylomata with no cellular atypia. HPV 6/11 was detected in 87% (13/15), and HPV 16/18 in 27% (4/15). In biopsies with cellular atypia, HPV types 6/11 were detected in 62% (8/13), HPV types 16/18 in 46% (6/13), and HPV types 31/33/35 or 51 in 50% (6/12). In about 50% of the biopsies where at least one hybridization method gave a positive result, either one of the commercial methods gave a negative result. Thus, in situ hybridization using digoxigenin-labelled HPV DNA probes is at least as sensitive a method for HPV detection in paraffin embedded biopsy tissue as in situ hybridization using biotin-labelled probes. Combination of in situ hybridization using digoxigenin-labelled HPV DNA probes with in situ hybridization with biotinylated HPV DNA probes may further improve sensitivity of HPV DNA detection in paraffin-embedded biopsy material.
The Value of Tests of Cure following Cervical Chlamydial Infection
Authors: White DJ , Mann CH , Matthews RS , Leeming J G , Clay J C 
Published: January 2017

Test of cure (TOC) was performed 2, 4 and 6 weeks after treatment for cervical chlamydia infection with 10–14 days of Deteclo one tablet twice daily, erythromycin 500 mg twice daily or doxycycline 100 mg twice daily. Testing was by chlamydia culture and IDEIA™ (DAKO diagnostics Ltd). Discrepant results were subsequently checked by immunofluorescence (Syva MicroTrak™) of both sets of left over transport media. Two hundred and three patients attended on at least one occasion; 189, 146 and 107 at 2, 4 and 6 weeks respectively. Of these 127, 70 and 34, respectively, denied sexual intercourse or had consistently used condoms. Fourteen were positive over the study period by either or both methods of detection. Of 8 culture positive results 3 were negative by IDEIA. Two of these had elementary bodies (EBs) on immunofluorescence of both sets of saved transport media. One had EBs on immunofluorescence of the saved culture transport medium only. None of the 6 IDEIA positive, culture negative patients had immunofluorescent EBs in the IDEIA transport media although one had EBs in the saved culture transport medium. One IDEIA suspicious, culture negative patient had EBs in both sets of saved transport media. There was no significant difference in the rate of chlamydia detection from patients admitting to or denying unprotected intercourse. TOC has a low yield in cases of cervical chlamydial infection when there has been careful contact tracing and treatment has been completed. If TOC is performed culture should be used if available and where antigen detection methods are used confirmation should be sought for any positive results.
Factors Associated with Trichomoniasis, Candidiasis and Bacterial Vaginosis
Authors: Hart Gavin 
Published: January 2017

Of 6125 women attending an STD clinic from 1988 to 1991, 5365 (88%) were tested for vaginitis of whom 97 (1.8%) had trichomoniasis, 945 (17.6%) had candidiasis, 734 (13.7%) had bacterial vaginosis and 3628 (67.6%) were free of vaginal infection. Dual infections occurred in 49 (0.9%) patients. Independent predictors for trichomoniasis by multivariate analysis were being pregnant (odds ratio (OR)=2.4), having vaginal discharge or dysuria (OR=4.7), being Aboriginal (OR=4.3), being Asian (OR=5.0), being unemployed (OR=2.1) or tattoed (OR=1.9). Many factors, including use of oral contraception (OR=1.2) and current antibiotic medication (OR=1.5), had a small significant association with candidiasis. Independent predictors for bacterial vaginosis were having multiple sex partners in the past month (OR=1.6), being unmarried (OR=1.5), being unemployed (OR=1.3) being a prostitute (OR=1.5) and not currently using antibiotic medication (OR=2.5). The epidemiological profiles were consistent with trichomoniasis and bacterial vaginosis being sexually transmitted diseases with epidemiology different from that of gonorrhoea and chlamydia and different from each other, and candidiasis being a disease in which constitutional factors are more important than issues relating to sexual transmission.
Assessing Statistics for the Measurement of Workload at a Genitourinary Medicine Clinic
Authors: Williams P 
Published: January 2017

Since January 1985 James Pringle House, the genitourinary medicine (GUM) clinic at London's Middlesex Hospital, has built up an extensive computerized patient database. Yearly statistics relating to 1) individual patients, 2) diagnoses, 3) attendances, and 4) an activity estimator (combining features of attendances and their corresponding diagnoses) are extracted. Each is assessed as a potential tool for quantifying the rising workload associated with the clinic's increased staffing levels and expanded services. Only the activity estimator identifies an upward trend, rising 26.3% from 1985–86 to 1991–92, caused largely by human immunodeficiency virus (HIV) and hepatitis B related attendances. This activity estimator could provide the basis for a workload reporting facility on all GUM computer systems. The 4 statistics exhibit other useful information, including a 21.0% rate of non-attendance (a substantial hidden addition to workload) and a strong allegiance to the clinic from patients residing outside of the local Health Authority region.
Endemic versus Non-Endemic Gonorrhoea in Stockholm: Results of Contact Tracing
Authors: Rudén A-K , Jonsson A , Lidbrink P , Allebeck P , Bygdeman S M 
Published: January 2017

The aim of the present study was to characterize endemic versus non-endemic gonorrhoea to identify risk groups for transmission and to evaluate the effects of intensified contact tracing performed by specially trained social workers at venereal clinics. A total of 671 gonorrhoea patients (283 women and 388 men) comprised the study group. Seventy percent of the women and 48% of the men had an endemic infection ( P<0.001). Men without a steady partner had an increased risk of non-endemic infection. A decrease from 75% to 40% was noted in the proportion of endemic infection in Stockholm from the first quarter of the study period (2 years) to the last. Contact tracing resulted in 1.2 identified partners per patient. A total of 736 partners were examined either as a result of contact tracing efforts or because they had sought medical care on their own prior to intervention. Forty-seven percent of these partners were infected, 44% were not infected and 9% were examined outside the study with results unknown to us. The partner notification efforts yielded 161 new untreated cases. Contact tracing of women generated one new case per 4.0 interviewed women and contact tracing of men one new case per 4.3 interviewed men. Interviewing index patients with endemic infection yielded the highest number of new cases. Forty-three percent of the patients were infected outside Stockholm but only a smaller part of these patients spread their infection further into the community. The experiences of the contact tracing and analysis of risk factors for transmission of gonorrhoea provide valuable knowledge that can be used to discourage spread of gonorrhoea.
Sexual Behaviour in the City of Lisbon
Authors: Amaro Fausto , Dantas Ana M. , da Cunha Teles Louise 
Published: January 2017

This study of sexual behaviour in the city of Lisbon is based on data obtained from 2 random samples, one of individuals aged 16–20 ( n = 400) and another of individuals over the age of 20 ( n = 400). Samples have been stratified by district, gender and age. Data were obtained by personal interview and by questionnaires completed confidentially by the interviewees. The data include the age of first sexual intercourse, the number of sexual partners, the prevalence of casual relations and the type of sexual practices according to sex and age group. Results show that despite the high risk of exposure to HIV in a significant percentage of the population, AIDS is seen as a personal threat by only 20% of the population. Six per cent of the adults (aged 21 and over) and 12% of the young people (16–20) replied that they had not yet considered the problem; 73.5% of the adults and 67.4% of the young people stated that they did not feel threatened by the disease. Data suggest that the differences between men and women have decreased. Among the sexually active population, only 12% of individuals under 21 and 8% of the adults use condoms regularly. The awareness of infidelity among couples is much lower than in reality. A significant discrepancy was found between the ‘awareness' and ‘reported reality’ for men and women who carry on casual extramarital relations: 14.9% of the women were convinced that their husbands or companions are unfaithful; 27.7% of the men reported they had been unfaithful in the years preceding the study; 3.7% of the men believed their wives had been unfaithful, whereas 8.9% of the women admitted to having been unfaithful over the same period. Data on sexual initiation reveal that contrary to young people (3.5%), 25.0% of the men over 20 begin their sexual life by resorting to prostitutes. At least one of the following behaviours can be attributed to two-thirds of the adults (21 and over) in the year preceding the study; extramarital relations; 2 or more sexual partners; did not use condoms; sexual relations with prosititutes, sexual relations with people whose sexual past they did not know. In conclusion, our study illustrates that the pattern of sexual behaviour in this population is associated to a significant risk of exposure to HIV and that a marked unawareness of such risk prevails. The differences in sexual behaviour patterns between men and women and between young and older people are important to formulate appropriate educational programmes.
Cervical Cytological Screening in HIV-Infected Women in Dublin—A Six-Year Review
Authors: Murphy M , Pomeroy L , Tynan M , Murphy J F , Mulcahy F M 
Published: January 2017

Summary: A retrospective study of the results of cervical cytological screening of HIV-infected women attending an inner city ambulatory HIV clinic over a 6-year period between 1987 and 1992 was carried out. During this time a total of 165 HIV-infected women attended for management of their HIV disease. The results of cervical cytological specimens (smears) were available in 136 (82.4%) women. The risk categories for HIV infection of these 136 women were intravenous drug use 110 (80.9%), heterosexual sex 24 (17.6%) and undetermined 2 (1.5%). Eighty-five (62.5%) of the 136 women were classified CDC group 2, 30 (22%) CDC group 3, and 21 (15.5%) CDC group 4 at the time of initial cytological screening. Forty-one (30.1%) women had mild dysplasia/CIN 1, 21 (15.4%) had moderate dysplasia/CIN 2 and 17 (12.5%) had severe dysplasia/CIN 3. The overall prevalence of dysplasia/CIN was 58.1%. Twenty-seven (34.2%) of the women with dysplasia/CIN had cytological evidence of human papillomavirus infection. No association between the clinical stage of HIV disease and the presence or degree of dysplasia/CIN was demonstrated. Women with cytological evidence of CIN were significantly more likely to have had genital warts than those with no evidence of CIN (OR 3.1, CI 1.1–10). In those women with cervical dysplasia who underwent colposcopic examination, CIN was confirmed in a high proportion of cases. The default rate from colposcopy, however, was high (35.4%).
The Activity of Candidate Virucidal Agents, Low pH and Genital Secretions against HIV-1 In Vitro
Authors: O'Connor T J , Kinchington D , Kangro H O , Jeffries D J 
Published: January 2017

Summary: The effect of low pH, normally present in the female genital tract, on HIV viability was examined. HIV is more acid stable than previously reported with no substantial reduction in infectivity occurring until pH levels are reduced below 4.5. The virucidal activity of 3 topical spermicides and chlorhexidine was assessed in vitro using previously established and newly modified assay systems. None of the agents tested had a selectivity index (SI) greater than 5.2. Semen and cervical secretions were assessed for their ability to inhibit HIV-1. While no virucidal effect was found in the latter, seminal fluid was found to have significant activity against HIV-1 and a SI of approximately 50.
HIV Seroprevalence in Healthy Blood Donors in Northeastern Zaire
Authors: Fischer Philip R , Toko Remy M 
Published: January 2017

Summary: Despite high seroprevalence rates in some parts of Africa, there is notable variation in prevalence between population subgroups. To document changes and trends in HIV seroprevalence in northeastern Zaire, 1989 to 1992 blood donor data were reviewed. Overall, 2453 donors were tested with seropositivity varying from 2.8% in 1989 to 6.9% in 1992. The increase in seropositivity was significant for men (2.5 to 5.8%, P = 0.017) and for people residing in rural areas (2.0 to 6.1%, P = 0.0008) but not for women (5.4 to 8.6%, P = 0.15) nor for urban individuals (10.5 to 8.6%, P = 0.55). These findings suggest that: 1) HIV infection is spreading in previously less-affected population subgroups rather than increasing widely in the entire population, 2) the HIV epidemic could be reaching a plateau or endemic phase in northeastern Zaire, and, 3) continued blood donor screening and wise transfusion practices are needed.
Patients Misrepresenting Their Risk Factors for AIDS
Authors: Brody Stuart 
Published: January 2017

Summary: To determine the truthfulness of patients' and research subjects' self-reports of their sexual and drug use histories in studies of human immunodeficiency virus (HIV) transmission and acquired immunodeficiency syndrome (AIDS) risk factors, studies of or pertinent to lying about AIDS risk factors were extracted from MEDLINE and PSYCLIT. The present paper describes normal and pathological motives for misrepresenting risk factors, and reviews the literature on such underreporting. There is much evidence for lying about anal intercourse and intravenous drug use risk factors, implying that the estimates of risk for vaginal transmission of HIV (particularly in Pattern-I industrialized countries) have been inflated. Research on HIV/AIDS behavioural risk factors must include sophisticated methods for the assessment of self-report validity, such as the use of behavioural markers, improved lie scales and interview methods, and physical methods.
Preoperative HIV Antibody Testing: The Views of Surgeons and Patients
Authors: Meadows Jean , Irving Gillian , Chapman Kate , Gazzard Brian , Catalan Jose 
Published: January 2017

Summary: Fifty surgeons and 72 preoperative patients in 2 central London hospitals were asked their views on preoperative HIV antibody testing, who is at risk of HIV infection and HIV test counselling. A substantial proportion of both surgeons and preoperative patients, 48% and 54% respectively, supported compulsory HIV antibody testing without patient consent. They believed this should occur as a routine practice prior to surgery. However, surgeons and patients differed in the preoperative patients to be considered for such testing. All surgeons advocating compulsory testing believed that it was only necessary for those patients belonging to high risk groups whilst preoperative patients believed that all patients should be HIV tested prior to surgery. Surgeons and patients also differed in their views on who was at risk of HIV infection. Surgeons believed those at risk to be members of identifiable risk groups whilst patients believed everyone was at risk, especially those engaging in high risk behaviours. Finally, when asked which health professionals they thought should be involved in HIV test counselling, both surgeons and patients agreed that hospital doctors, including surgeons themselves, should take on the responsibility of test counselling. The implications of this and routine HIV antibody testing are discussed.
Enzyme-based assay for quantification of paraoxon in blood of parathion poisoned patients
Authors: Eyer Florian , Eyer Peter 
Published: January 2017

1 Paraoxon concentration was estimated by means of inhibition kinetics observed with electric eel acetylcholinesterase (AChE) which was determined by a modified Ellman procedure. In human plasma, paraoxon was stabilized by inactivation of paraoxonase with EDTA and aluminon and by inhibition of butyrylcholinesterase with ethopropazine. Paraoxon (1-50 ng) was recovered at 86+1.7% (mean+s.e.m.) in ether extracts from 0.5 ml samples of spiked stabilized plasma. It could be stored without loss at 7208C for at least 1 month. 2 The enzyme-based assay was applied to follow the paraoxon plasma concentrations in three suicidal patients with severe parathion poisoning. In poisoning with excessive doses and initial paraoxon concentrations above 500 nM, therapeutic obidoxime concentrations of approximately 10 mM failed to essentially reactivate erythrocyte AChE in vivo, while reactivat-ability ex vivo was nearly complete. With the plasma concentrations of paraoxon dropping below 100 nM, however, reactivation by obidoxime became signifi-cant. Unexpectedly, paraoxon levels occasionally re-increased during treatment and resulted in re-inhibition of AChE, bearing some resemblance to the Intermediate Syndrome. 3 The paraoxon concentrations measured fitted satisfactorily the values calculated from the kinetic constants previously obtained for AChE inhibition and obidoxime-induced reactivation in vitro. This indicates that diethylphosphoryloxime formation during obidoxime-induced reactivation does not markedly contribute to the re-inhibition of AChE as observed in vitro.
Microsomal metabolism of carbamazepine and oxcarbazepine in liver and placenta
Authors: Myllynen P , Pienimäki P , Raunio H , Vähäkangas K 
Published: January 2017

Metabolism of both carbamazepine (CBZ) and oxcarbaze-pine (OCBZ) were catalyzed by human liver microsomes and microsomes from livers of CBZ-induced or non-induced C57BL/6 mice. Human placental microsomes metabolized only OCBZ. Mouse liver microsomes metabolized CBZ to carbamazepine-10,11-epoxide (CBZ-E), 10- hydroxy-10,11-dihydro-carbamazepine (10-OH-CBZ), 3- hydroxy-carbamazepine (3-OH-CBZ), 10,11-trans-dihydroxy-10,11-dihydro-carbamazepine (10,11-D) and to an unidentified metabolite. CBZ-pretreatment of mice increased both ethoxyresorufin O-deethylase activity in the liver and the amount of CBZ-E in microsomal incubations regardless of the age of mice. Human liver microsomes catalyzed the formation of CBZ to 9-hydroxymethyl-10-carbamoyl acridan (9-AC) in addition to CBZ-E, 3-OH-CBZ and 10-OH-CBZ. OCBZ was metabolized to its active metabolite in all incubations. An unknown metabolite was also present in some of the incubations. Human liver microsomes catalyzed only minute covalent binding of CBZ and OCBZ to DNA. Binding of OCBZ was, however, one order of magnitude greater than binding of CBZ. Human placental micro-somes from the mothers on CBZ therapy did not catalyze CBZ metabolism. The same microsomes catalyzed OCBZ metabolism to 10-OH-CBZ and to an unknown metabolite. These results indicate autoinduction in CBZ metabolism in mouse liver. Due to the higher binding of OCBZ than CBZ to DNA in vitro, further studies on the potential mutagenicity of OCBZ may be warranted.
Determination of glutathione S-transferase μ and τ polymorphisms in neurological disease
Authors: Stroombergen Maudy CMJ , Waring Rosemary H 
Published: January 2017

1 Correlations between deletions in two glutathione S-transferase (GST) genes, GSTM1 and GSTT1 and susceptibility to Alzheimer's disease (AD), motor neuron disease (MND) and Parkinson's disease (PD) have been investigated by PCR, using primers specific for both genes. 2 It was found that males with a deletion of the GSTM1 gene were more susceptible to PD and males with a deletion of the GSTT1 gene more susceptible to MND and PD, possibly implying that environmental factors which specifically target men may be involved. Furthermore, subjects with a deletion of the GSTT1 gene were more susceptible to AD.
IGF-I in experimental daunorubicin-induced cardiomyopathy in rabbits
Authors: Geršl V , Cerman J , Šuba P , Mazurová Y , Hrdina R , Macháčková J 
Published: January 2017

1 The occurrence of IGF-I was investigated in rabbits with experimentally daunorubicin-induced cardiomyopathy. IGF-I was measured in the heart, serum, liver and skeletal muscle. 2 A significant increase in the IGF-I was found in the left heart ventricle in daunorubicin cardiomyopathy (152.9±10.0 ng/g vs 95.1±4.2 ng/g in the control group). This site of increased IGF-I activity corresponded well with the maximum of morphological changes (dispersed cytolysis of cardiomyocytes mostly without developed subsequent interstitial myofibrosis). 3 The highest levels of IGF-I were present in right and left cardiac atrium (but without significant differences between the groups). Furthermore, in skeletal muscle, the levels of IGF-I in the daunorubicin group (839.0±142.1 ng/g) were significantly higher in comparison with the control group (482.5±83.1 ng/g). 4 The level of IGF-I in the left ventricle in the daunorubicin group (but not in the control group) was significantly higher than that in the liver. There were no correlations observed between the levels of IGF-I in the heart and in the serum. 5 The increase in IGF-I concentrations in the left heart ventricle after the administration of daunorubicin may thus reflect possible autocrine/paracrine role of IGF-I in cardiomyopathy.