bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Diagnostic Value of 3-Dimensional Sampling Perfection With Application Optimized Contrasts Using Different Flip Angle Evolutions (SPACE) MRI in Evaluating Lower Extremity Deep Venous Thrombus
Authors: Wu Gang , Xie Ruyi , Zhang Xiaoli , Morelli John , Yan Xu , Zhu Xiaolei , Li Xiaoming 
Published: July 2017

Objectives
The aim of this study was to evaluate the diagnostic performance of noncontrast magnetic resonance imaging utilizing sampling perfection with application optimized contrasts using different flip angle evolutions (SPACE) in detecting deep venous thrombus (DVT) of the lower extremity and evaluating clot burden.

Materials and Methods
This prospective study was approved by the institutional review board. Ninety-four consecutive patients (42 men, 52 women; age range, 14–87 years; average age, 52.7 years) suspected of lower extremity DVT underwent ultrasound (US) and SPACE. The venous visualization score for SPACE was determined by 2 radiologists independently according to a 4-point scale (1–4, poor to excellent). The sensitivity and specificity of SPACE in detecting DVT were calculated based on segment, limb, and patient, with US serving as the reference standard. The clot burden for each segment was scored (0–3, patent to entire segment occlusion). The clot burden score obtained with SPACE was compared with US using a Wilcoxon test based on region, limb, and patient. Interobserver agreement in assessing DVT (absent, nonocclusive, or occlusive) with SPACE was determined by calculating Cohen kappa coefficients.

Results
The mean venous visualization score for SPACE was 3.82 ± 0.50 for reader 1 and 3.81 ± 0.50 for reader 2. For reader 1, sensitivity/specificity values of SPACE in detecting DVT were 96.53%/99.90% (segment), 95.24%/99.04% (limb), and 95.89%/95.24% (patient). For reader 2, corresponding values were 97.20%/99.90%, 96.39%/99.05%, and 97.22%/95.45%. The clot burden assessed with SPACE was not significantly different from US (P > 0.05 for region, limb, patient). Interobserver agreement of SPACE in assessing thrombosis was excellent (kappa = 0.894 ± 0.014).

Conclusions
Non–contrast-enhanced 3-dimensional SPACE magnetic resonance imaging is highly accurate in detecting lower extremity DVT and reliable in the evaluation of clot burden. SPACE could serve as an important alternative for patients in whom US cannot be performed.
Male Fertility After Inguinal Hernia Mesh Repair
Authors: Kohl Andreas Pagh , Andresen Kristoffer , Rosenberg Jacob 
Published: July 2017

Objective:
To determine whether patients who receive an inguinal hernia repair father the same number of children as the background population.

Background:
Although the effect of inguinal hernia repair on male fertility has previously been investigated through indirect measures, no previous studies have evaluated the final measure of male fertility, which is the number of children fathered by patients.

Methods:
Prospectively collected data on 32,621 male patients between the ages of 18 and 55 years who received 1 or more inguinal hernia repairs during the years 1998 to 2012 were found in 5 comprehensive Danish linked registers. Patients were matched with 97,805 controls, and the number of fathered children was recorded as the primary outcome.

Results:
Patients who were operated unilaterally fathered more children than controls (156 vs 147 children per 1000 individuals, P = 0.02), whereas patients who were operated bilaterally fathered the same number of children as controls. Unilateral Lichtenstein operation resulted in an increase in number of children fathered by patients (161 vs 151 children per 1000 patients, P = 0.009). No difference in the number of children fathered was found for any year following operation. Meanwhile, time between operation and first child was longer among controls than patients (log-rank P = 0.003). The youngest (18–30 years of age) bilaterally operated patients fathered the same number of children as controls.

Conclusions:
Patients who underwent inguinal hernia repair using Lichtenstein technique or laparoscopic approach did not father fewer children than expected. Thus, inguinal hernia repair using Lichtenstein or laparoscopic approach did not impair male fertility.
Venous thromboembolism after major venous injuries: Competing priorities
Authors: Frank Brian , Maher Zoё , Hazelton Joshua P. , Resnick Shelby , Dauer Elizabeth , Goldenberg Anna , Lubitz Andrea L. , Smith Brian P. , Saillant Noelle N. , Reilly Patrick M. , Seamon Mark J. 
Published: July 2017

BACKGROUND
Venous thromboembolism (VTE) after major vascular injury (MVI) is particularly challenging because the competing risk of thrombosis and embolization after direct vessel injury must be balanced with risk of bleeding after surgical repair. We hypothesized that venous injuries, repair type, and intraoperative anticoagulation would influence VTE formation after MVI.

METHODS
A multi-institution, retrospective cohort study of consecutive MVI patients was conducted at three urban, Level I centers (2005–2013). Patients with MVI of the neck, torso, or proximal extremities (to elbows/knees) were included. Our primary study endpoint was the development of VTE (DVT or pulmonary embolism [PE]).

RESULTS
The 435 major vascular injury patients were primarily young (27 years) men (89%) with penetrating (84%) injuries. When patients with (n = 108) and without (n = 327) VTE were compared, we observed no difference in age, mechanism, extremity injury, tourniquet use, orthopedic and spine injuries, damage control, local heparinized saline, or vascular surgery consultation (all p > 0.05). VTE patients had greater Injury Severity Score (ISS) (17 vs. 12), shock indices (1 vs. 0.9), and more torso (58% vs. 35%) and venous (73% vs. 48%) injuries, but less often received systemic intraoperative anticoagulation (39% vs. 53%) or postoperative enoxaparin (47% vs. 61%) prophylaxis (all p < 0.05). After controlling for ISS, hemodynamics, injured vessel, intraoperative anticoagulation, and postoperative prophylaxis, multivariable analysis revealed venous injury was independently predictive of VTE (odds ratio, 2.7; p = 0.002). Multivariable analysis of the venous injuries subset (n = 237) then determined that only delay in starting VTE chemoprophylaxis (odds ratio, 1.3/day; p = 0.013) independently predicted VTE after controlling for ISS, hemodynamics, injured vessel, surgical subspecialty, intraoperative anticoagulation, and postoperative prophylaxis. Overall, 3.4% of venous injury patients developed PE, but PE rates were not related to their operative management (p = 0.72).

CONCLUSION
Patients with major venous injuries are at high risk for VTE, regardless of intraoperative management. Our results support the immediate initiation of postoperative chemoprophylaxis in patients with major venous injuries.

LEVEL OF EVIDENCE
Therapeutic/care management, level IV.
Conformational locking of Ufm1 upon binding to the Ufm1-interacting sequence of Uba5
Authors: Kelly Ryan T. , Oweis Walaa , Wiener Reuven , Berndsen Christopher E. 
Published: July 2017

AbstractUbiquitin fold modifier 1 (Ufm1) is a ubiquitin-like protein (UBL) found in eukaryotic organisms which plays a crucial role in ER stress management and signal transduction. The crystal structure of UFM1 and its E1 (Uba5) in complex shows that Ufm1 binds to the adenylation domain of UBA5 and interacts with a separate Ufm1-interacting sequence (UIS) in the C-terminus of UBA5. The UIS interacts with Ufm1 on the opposite side of Ufm1 protein from the adenylation domain of Uba5 and the reason for this second interaction site is unclear. We analyzed Ufm1 bound to the UIS sequence through molecular dynamics simulations in order to identify additional functions for this interaction. We found that the residues in the adenylation interaction site of Ufm1 have less movement when the UIS peptide was bound to Ufm1 and formed a structure that aligns well with Ufm1 bound to the Uba5 adenylation domain. We further identified an amino acid that connects the UIS to the adenylation domain interacting site. Mutation of this amino acid decreases charging activity and shifts the Ufm1 conformation population toward the unlocked configuration even in the presence of the UIS peptide. These data suggest a role for the Uba5 UIS in stimulating activation of Ufm1.
Feedback regulation of steady-state epithelial turnover and organ size
Authors: Liang Jackson , Balachandra Shruthi , Ngo Sang , O’Brien Lucy Erin 
Published: July 2017

ABSTRACTEpithelial organs undergo steady-state turnover throughout adult life, with old cells being continually replaced by the progeny of stem cell divisions1. To avoid hyperplasia or atrophy, organ turnover demands strict equilibration of cell production and loss2-4. However, the mechanistic basis of this equilibrium is unknown. Using the adultDrosophilaintestine5, we find that robustly precise turnover arises through a coupling mechanism in which enterocyte apoptosis breaks feedback inhibition of stem cell divisions. Healthy enterocytes inhibit stem cell division through E-cadherin, which prevents secretion of mitogenic EGFs by repressing transcription of the EGF maturation factorrhomboid. Individual apoptotic enterocytes promote divisions by loss of E-cadherin, which releases cadherin-associated β-catenin/Armadillo and p120-catenin to inducerhomboid. Induction ofrhomboidin the dying enterocyte triggers EGFR activation in stem cells within a discrete radius. When we block apoptosis, E-cadherin-controlled feedback suppresses divisions, and the organ retains the same number of cells. When we disrupt feedback, apoptosis and divisions are uncoupled, and the organ develops either hyperplasia or atrophy. Altogether, our work demonstrates that robust cellular balance hinges on the obligate coupling of divisions to apoptosis, which limits the proliferative potential of a stem cell to the precise time and place that a replacement cell is needed. In this manner, localized cell-cell communication gives rise to tissue-level homeostatic equilibrium and constant organ size.
The use of a chimeric rhodopsin vector for the detection of new proteorhodopsins based on color
Authors: Pushkarev Alina , Hevroni Gur , Roitman Sheila , Shim Jin-gon , Choi Ahreum , Jung Kwang-Hwan , Béjà Oded 
Published: July 2017

AbstractStudent microbial ecology laboratory courses are often conducted as condensed courses in which theory and wet lab work are combined in a very intensive short time period. In last decades, the study of marine microbial ecology is increasingly reliant on molecular-based methods, and as a result many of the research projects conducted in such courses require sequencing that is often not available on site and may take more time than a typical course allows. In this work, we describe a protocol combining molecular and functional methods for analyzing proteorhodopsins (PRs), with visible results in only 4-5 days, that do not rely on sequencing. PRs were discovered in oceanic surface waters two decades ago, and have since been observed in different marine environments and diverse taxa, including the abundant alphaproteobacterial SAR11 group. PR subgroups are currently known to absorb green and blue light, and their distribution was previously explained by prevailing light conditions - green pigments at the surface and blue pigments in deeper waters, as blue light travels deeper in the water column. To detect PR in environmental samples, we created a chimeric plasmid suitable for direct expression of PRs using PCR amplification and functional analysis inEscherichia colicells. Using this assay, we discovered several exceptional cases of PRs whose phenotypes differed from those predicted based on sequence only, including a previously undescribed yellow-light absorbing PRs. We applied this assay in two 10-days marine microbiology courses and found it to greatly enhance students’ laboratory experience, enabling them to gain rapid visual feedback and colorful reward for their work. Furthermore we expect this assay to promote the use of functional assays for the discovery of new rhodopsin variants.
Sex- and genotype-effects on nutrient-dependent fitness landscapes inDrosophila melanogaster
Authors: Camus M. Florencia , Fowler Kevin , Piper Matthew W.D. , Reuter Max 
Published: July 2017

AbstractThe sexes perform different reproductive roles and have evolved sometimes strikingly different phenotypes. One focal point of adaptive divergence occurs in the context of diet and metabolism, and males and females of a range of species have been shown to require different nutrients to maximise their fitness. Biochemical analyses inDrosophila melanogasterhave confirmed that dimorphism in dietary requirements is associated with molecular sex-differences in metabolite titres. In addition, they also showed significant within-sex genetic variation in the metabolome. To date however, it is unknown whether this metabolic variation translates into differences in reproductive fitness. The answer to this question is crucial to establish whether genetic variation is selectively neutral or indicative of constraints on sex-specific physiological adaptation and optimisation. Here we assay genetic variation in consumption and metabolic fitness effects by screening male and female fitness of thirtyD. melanogastergenotypes across four protein-to-carbohydrate ratios. In addition to confirming sexual dimorphism in consumption and fitness, we find significant genetic variation in male and female dietary requirements. Importantly, these differences are not explained by feeding responses and most likely reflect metabolic variation that, in turn, suggest the presence of genetic constraints on metabolic dimorphism.
Multi-method brain imaging reveals impaired representations as well as altered connectivity in adults with dyscalculia
Authors: Bulthé Jessica , Prinsen Jellina , Vanderauwera Jolijn , Duyck Stefanie , Daniels Nicky , Gillebert Céline R. , Mantini Dante , Op de Beeck Hans P. , De Smedt Bert 
Published: July 2017

SummaryTwo hypotheses have been proposed about the etiology of neurodevelopmental disorders: representation impairments versus disrupted access to representations. We implemented a multi-method brain imaging approach to directly compare the representation vs. access hypotheses in dyscalculia, a highly prevalent but understudied neurodevelopmental disorder in learning to calculate. We combined several magnetic resonance imaging methods and analyses, including multivariate analyses, functional and structural connectivity, and voxel-based morphometry analysis, in a sample of 24 adults with dyscalculia and 24 carefully matched controls. Results showed a clear deficit in the non-symbolic magnitude representations in parietal, temporal, and frontal regions in dyscalculia. We also observed hyper-connectivity in visual brain regions and increased grey matter volume in the default mode network in adults with dyscalculia. Hence, dyscalculia is related to a combination of diverse neural markers which are altogether distributed across a substantial portion of cerebral cortex, supporting a multifactorial model of this neurodevelopmental disorder.
Chill coma in the locust,Locusta migratoria, is initiated by spreading depolarization in the central nervous system
Authors: Robertson R. Meldrum , Spong Kristin E. , Srithiphaphirom Phinyaphat 
Published: July 2017

AbstractThe ability of chill-sensitive insects to function at low temperatures limits their geographic ranges. They have species-specific temperatures below which movements become uncoordinated prior to entering a reversible state of neuromuscular paralysis. In spite of decades of research, which in recent years has focused on muscle function, the role of neural mechanisms in detemining chill coma is unknown. Spreading depolarization (SD) is a phenomenon that causes a shutdown of neural function in the integrating centres of the central nervous system. We investigated the role of SD in the process of entering chill coma in the locust,Locusta migratoria. We used thermolimit respirometry and electromyography in whole animals and extracellular and intracellular recording techniques in semi-intact preparations to characterize neural events during chilling. We show that chill-induced SD in the central nervous system is the mechanism underlying the critical thermal minimum for coordinated movement in locusts. This finding will be important for understanding how insects adapt and acclimate to changing environmental temperatures.
Traction force screening enabled by compliant PDMS elastomers
Authors: Yoshie Haruka , Koushki Newsha , Kaviani Rosa , Rajendran Kavitha , Dang Quynh , Husain Amjad , Yao Sean , Li Chuck , Sullivan John K , Saint-Geniez Magali , Krishnan Ramaswamy , Ehrlicher Allen J 
Published: July 2017

AbstractActo-myosin contractility is an essential element of many aspects of cellular biology, and manifests as traction forces that cells exert on their surroundings. The central role of these forces makes them a novel principal therapeutic target in diverse diseases. This requires accurate and higher capacity measurements of traction forces; however, existing methods are largely low throughput, limiting their utility in broader applications. To address this need, we employ Fourier-transform traction force microscopy in a parallelized 96-well format, which we refer to as contractile force screening (CFS).Critically, rather than the frequently employed hydrogel polyacrylamide (PAA), we fabricate these plates using polydimethylsiloxane (PDMS) rubber. Key to this approach is that the PDMS used is very compliant, with a lower-bound Young’s modulus of approximately 0.7 kPa. We subdivide these monolithic substrates spatially into biochemically independent wells, creating a uniform multiwell platform for traction force screening. We demonstrate the utility and versatility of this platform by quantifying the compound and dose-dependent contractility responses of human airway smooth muscle cells and retinal pigment epithelial cells.
Package graphic design development
Authors: Hamlin Robert P. , Gin Michael , Nyhof Fiona , Bogue Joe 
Published: July 2017

Purpose
The purpose of this paper is to establish whether consumer focus groups can generate reliable data when used as a consumer research input to copy/graphic design development for retail food packaging.

Design/methodology/approach
Six focus groups of six consumers each (n=36) were used to assess four concept designs for a manuka honey cordial product. The focus groups were used to rank the design concepts and to generate specific consumer-driven recommendations to develop/improve each of the designs. A new version of each design was then developed using these recommendations. The eight designs (four original and four improved) were tested together, using a quantitative field experiment at a supermarket in the area from which the focus group sample was drawn.

Findings
The results showed that the focus group rankings of the four original designs were largely predictive of the outcomes of the field trial. The improved designs also consistently outperformed their original equivalents in the field trials. Very large improvements were recorded with respect to the weaker original designs.

Research limitations/implications
The research only studied a single product type. However, the results demonstrate that focus group outcomes can be extended onto larger populations if the focus group sample is large enough.

Practical implications
These results support the use of consumer focus groups as a research input to package graphic design development.

Originality/value
Focus groups are used regularly in research where the applicability of their results to larger populations is assumed. This is the first research to formally test that assumption.
The five-factor traits as moderators between job insecurity and health
Authors: Iliescu Dragos , Macsinga Irina , Sulea Coralia , Fischmann Gabriel , Vander Elst Tinne , De Witte Hans 
Published: July 2017

Purpose
The purpose of this paper is to analyze the moderating effects of the broad personality traits associated with the five-factor model (FFM) of personality, on the relationship between qualitative and quantitative job insecurity (JI) and physical and mental health complaints.

Design/methodology/approach
Self-report data collected in a cross-sectional study from a heterogeneous sample of 469 Romanian employees was analyzed with hierarchical regressions in order to identify moderation effects between each personality trait, JI and health outcomes.

Findings
Neuroticism and introversion amplify the relationship between JI and mental health complaints. None of the other personality traits showed any significant interaction with JI. No moderating effects were found for physical health complaints. Quantitative and qualitative JI show a high correlation and similar relationships with other variables, but may not be part of the same larger factor.

Practical implications
The FFM has a lower contribution than expected in explaining the JI-health dynamic, with only 2 out of 5 reaching significance. The personality traits of neuroticism and introversion function as moderately strong vulnerability factors in the JI-mental health relationship, and may be used by managers in identifying employees who are at risk in situations when JI is likely to appear.

Originality/value
The authors offer overall support for the main effect model in the relationship between JI and health, showing that, while some broad personality traits buffer the negative effect of JI in a fairly strong manner, this effect may be very difficult to completely abolish. The authors further show that quantitative and qualitative JI are very closely related facets of the broader JI construct.
The implications of work-family balance among dual-earner couples
Authors: Adisa Toyin Ajibade , Osabutey Ellis L.C. , Gbadamosi Gbolahan 
Published: July 2017

PurposeThe implications of the work-family balance (WFB) of dual-earner couples are well known; however, the extant literature on this topic has failed to adequately explore the context of Sub-Saharan Africa (SSA), specifically Nigeria. The purpose of this paper is to examine the implications of the WFB of dual-earner couples in Nigeria.Design/methodology/approachThis paper employs a qualitative methodology in order to explore the effect of couples’ dual-earner status on their WFB in an African context by using Nigerian medical practitioners as the empirical focus.FindingsThe findings reveal that the dual-earner status provides some respite from financial hardship and improves family finances, which subsequently enhances WFB. However, the dual-earner status also has negative impacts on WFB in terms of work performance, dysfunctionality, and associated societal problems.Originality/valueThis paper provides insights into the WFB of dual-earner couples in the non-western context of SSA, highlighting the previously unexplored implications of dual-earner status in the context of SSA.