bluuebunny/crossref_metadata_2025_split huggingface dataset:
A guanidine-appended scyllo-inositol derivative AAD-66 enhances brain delivery and ameliorates Alzheimer’s phenotypes
Authors: Lee Dohyun , Lee Woo-Sirl , Lim Sungsu , Kim Yun Kyung , Jung Hoe-Yune , Das Sanket , Lee Juhyun , Luo Wenjie , Kim Kyong-Tai , Chung Sung-Kee 
Published: October 2017

AbstractAlzheimer’s disease (AD) is a degenerative brain disease that destroys memory and other important mental functions but lacks efficient therapeutic agents. Blocking toxic amyloid β (Aβ) could be beneficial for AD and represents a promising therapeutic strategy for AD treatment. scyllo-Inositol (SI) is a potential therapeutic for AD by directly interacting with the Aβ peptide to inhibit Aβ42 fiber formation. Clinical studies of SI showed promising benefits on mild to moderate AD, however, with limitations on dosage regime. A new strategy to enhance the brain delivery of SI is needed to achieve the efficacy with minimum adverse effects. Herein, we report that a novel guanidine-appended SI derivative AAD-66 resulted in more effective reductions of brain Aβ and plaque deposits, gliosis, and behavioral memory deficits in the disease-established 5xFAD mice. Overall, our present study reveals the potential of AAD-66 as a promising therapeutic agent for AD.
Label-free analysis of the characteristics of a single cell trapped by acoustic tweezers
Authors: Kim Min Gon , Park Jinhyoung , Lim Hae Gyun , Yoon Sangpil , Lee Changyang , Chang Jin Ho , Shung K. Kirk 
Published: October 2017

AbstractSingle-cell analysis is essential to understand the physical and functional characteristics of cells. The basic knowledge of these characteristics is important to elucidate the unique features of various cells and causative factors of diseases and determine the most effective treatments for diseases. Recently, acoustic tweezers based on tightly focused ultrasound microbeam have attracted considerable attention owing to their capability to grab and separate a single cell from a heterogeneous cell sample and to measure its physical cell properties. However, the measurement cannot be performed while trapping the target cell, because the current method uses long ultrasound pulses for grabbing one cell and short pulses for interrogating the target cell. In this paper, we demonstrate that short ultrasound pulses can be used for generating acoustic trapping force comparable to that with long pulses by adjusting the pulse repetition frequency (PRF). This enables us to capture a single cell and measure its physical properties simultaneously. Furthermore, it is shown that short ultrasound pulses at a PRF of 167 kHz can trap and separate either one red blood cell or one prostate cancer cell and facilitate the simultaneous measurement of its integrated backscattering coefficient related to the cell size and mechanical properties.
Psychotropic in the environment: risperidone residues affect the behavior of fish larvae
Authors: Kalichak Fabiana , Idalencio Renan , da Rosa João Gabriel Santos , Barcellos Heloísa Helena de Alcântara , Fagundes Michele , Piato Angelo , Barcellos Leonardo José Gil 
Published: October 2017

AbstractThe ability to avoid and escape from predators are clearly relevant behaviors from the ecological perspective and directly interfere with the survival of organisms. Detected in the aquatic environment, risperidone can alter the behavior of exposed species. Considering the risk of exposure in the early stages of life, we exposed zebrafish embryos to risperidone during the first 5 days of life. Risperidone caused hyperactivity in exposed larvae, which in an environmental context, the animals may be more vulnerable to predation due to greater visibility or less perception of risk areas.
Field evidence for transfer of plastic debris along a terrestrial food chain
Authors: Huerta Lwanga Esperanza , Mendoza Vega Jorge , Ku Quej Victor , Chi Jesus de los Angeles , Sanchez del Cid Lucero , Chi Cesar , Escalona Segura Griselda , Gertsen Henny , Salánki Tamás , van der Ploeg Martine , Koelmans Albert A. , Geissen Violette 
Published: October 2017

AbstractAlthough plastic pollution happens globally, the micro- (<5 mm) and macroplastic (5–150 mm) transfer of plastic to terrestrial species relevant to human consumption has not been examined. We provide first-time evidence for micro- and macroplastic transfer from soil to chickens in traditional Mayan home gardens in Southeast Mexico where waste mismanagement is common. We assessed micro- and macroplastic in soil, earthworm casts, chicken feces, crops and gizzards (used for human consumption). Microplastic concentrations increased from soil (0.87 ± 1.9 particles g−1), to earthworm casts (14.8 ± 28.8 particles g−1), to chicken feces (129.8 ± 82.3 particles g−1). Chicken gizzards contained 10.2 ± 13.8 microplastic particles, while no microplastic was found in crops. An average of 45.82 ± 42.6 macroplastic particles were found per gizzard and 11 ± 15.3 macroplastic particles per crop, with 1–10 mm particles being significantly more abundant per gizzard (31.8 ± 27.27 particles) compared to the crop (1 ± 2.2 particles). The data show that micro- and macroplastic are capable of entering terrestrial food webs.
Potential of mean force between oppositely charged nanoparticles: A comprehensive comparison between Poisson–Boltzmann theory and Monte Carlo simulations
Authors: Zhang Jin-Si , Zhang Xi , Zhang Zhong-Liang , Tan Zhi-Jie 
Published: October 2017

AbstractIon-mediated interactions between like-charged polyelectrolytes have been paid much attention, and the Poisson–Boltzmann (PB) theory has been shown to fail in qualitatively predicting multivalent ion-mediated like-charge attraction. However, inadequate attention has been paid to the ion-mediated interactions between oppositely charged polyelectrolytes. In this work, the potentials of mean force (PMF) between oppositely charged nanoparticles in 1:1 and 2:2 salt solutions were investigated by Monte Carlo simulations and the PB theory. Our calculations show that the PMFs between oppositely charged nanoparticles are generally attractive in 1:1 and 2:2 salt solutions and that such attractive PMFs become weaker at higher 1:1 or 2:2 salt concentrations. The comprehensive comparisons show that the PB theory can quantitatively predict the PMFs between oppositely charged nanoparticles in 1:1 salt solutions, except for the slight deviation at very high 1:1 salt concentration. However, for 2:2 salt solutions, the PB theory generally overestimates the attractive PMF between oppositely charged nanoparticles, and this overestimation becomes more pronounced for nanoparticles with higher charge density and for higher 2:2 salt concentration. Our microscopic analyses suggest that the overestimation of the PB theory on the attractive PMFs for 2:2 salt solutions is attributed to the underestimation of divalent ions bound to nanoparticles.
Clinical evaluation of corneal changes after phacoemulsification in diabetic and non-diabetic cataract patients, a systematic review and meta-analysis
Authors: Tang Yizhen , Chen Xinyi , Zhang Xiaobo , Tang Qiaomei , Liu Siyu , Yao Ke 
Published: October 2017

AbstractCorneal endothelium morphological abnormalities result in fluid imbalance, stromal swelling, and loss of transparency, thus impairing visual function. Recently, growing number of studies have focused on diabetic corneal abnormalities after cataract surgery and its comparison with non-diabetic patients, the results remain conflicting. Thus, to evaluate the effect of phacoemulsification on the corneal properties in diabetic and non-diabetic patients, prospective studies were comprehensively searched through PubMed, EMBASE, and Cochrane databases updated to Jan 2017. A meta-analysis of the 13 identified studies was performed using weighted mean difference (WMD) and 95% confidence interval (CI). For the dynamic changes between preoperative and postoperative values, significant differences were identified between the two groups in endothelial cell density (ECD) and hexagon cells (HC%) at 1 day, 1 week, 1 month, and 3 months postoperatively, in central corneal thickness (CCT) at 1 month postoperatively, and in coefficient variation (CV) at 1 week and 1 month postoperatively. However, no significant differences were observed in CCT at 1 day, 1 week and 3 months postoperatively or in CV at 1 day and 3 months postoperatively. Diabetic corneas are more vulnerable to stress and trauma, resulting in greater morphological abnormalities and longer recovery time.
The polysemy network of Chinese ‘one’-phrases in a diachronic constructional perspective
Authors: Chen I-Hsuan 
Published: October 2017

AbstractThis study investigates the development of a polysemy network within a constructional framework. The synchronic variation of Chinese ‘one’-phrases is explained by diachronic developments. The results of a quantitative corpus analysis show that each of the senses of the ‘one’ phrase tends to occur in specific syntactic constructions due to inheritance from extant constructions. The results contribute to explaining the formation of a diachronic polysemy network by investigating the hierarchical structure of its constructions, thus allowing a deeper understanding of how semantic extensions have been formed through gradual constructional association.
On the grammaticalization of Finnish colorative construction
Authors: Hamunen Markus Veli Juhani 
Published: October 2017

AbstractThis paper concentrates on the diachronic development of the so-called Colorative Construction (CoC) in Finnish, a two-verb expression consisting of an A-infinitive and an ideophonically based descriptive (or ‘colorative’) finite verb, e.g.susijuos-ta jolkottele-e[wolf run-inf col-prs.3sg] ‘wolf runs trotting’. The paper combines variationist dialectal data, grammaticalization theory, and Construction Grammar formalization. The detailed diachronic description demonstrates that the development from proto-CoC to modern CoC is the epitome of constructionalization, i.e., a gradual process of grammatical changes whereby both the form and the function of an existing construction are altered, creating a new expression type. Major changes in the Balto-Finnic case system were the primary force behind this process. Constructionalization of the CoC itself included the first syntagmatic changes through reanalysis. This gradually created a new paradigmatic expression type, followed by paradigmatic extension through analogy, which widened the frame semantics of the newly coined type.
The probability of causation1
Authors: Dawid A Philip , Musio Monica , Murtas Rossella 
Published: October 2017

Abstract
Many legal cases require decisions about causality, responsibility or blame, and these may be based on statistical data. However, causal inferences from such data are beset by subtle conceptual and practical difficulties, and in general it is, at best, possible to identify the ‘probability of causation’ as lying between certain empirically informed limits. These limits can be refined and improved if we can obtain additional information, from statistical or scientific data, relating to the internal workings of the causal processes. In this article we review and extend recent work in this area, where additional information may be available on covariate and/or mediating variables.
Functional Role of Lacrimal Gland Fibroblasts in a Mouse Model of Chronic Graft-Versus-Host Disease
Authors: Yamane Mio , Ogawa Yoko , Mukai Shin , Yaguchi Saori , Kamijuku Hajime , Inaba Takaaki , Asai Kazuki , Morikawa Satoru , Kawakami Yutaka , Shimmura Shigeto , Tsubota Kazuo 
Published: October 2017

Purpose:
This study aimed to clarify the mechanisms and assess the characteristics of the chronic graft-versus-host disease (cGVHD) fibrosis in the lacrimal gland (LG) of mice.

Methods:
Histopathology of LG tissues was examined by immunohistochemistry and electron microscopy. Cultured fibroblasts derived from the LG were analyzed by phase-contrast microscopy, immunocytochemistry, flow cytometry, proliferation assay, and invasion and migration assays.

Results:
Cultured murine LG fibroblasts in cGVHD were spindle-shaped and relatively small, whereas those from syngeneic controls were polygon-shaped and relatively large. Flow cytometric analysis showed that the LG fibroblasts in cGVHD had elevated HSP47 levels. The LG fibroblasts in cGVHD also showed increased expression of major histocompatibility complex class II. Furthermore, the proportion of Sca-1+PDGFR-α+ cells among the LG fibroblasts in cGVHD was considerably increased compared with controls. Cell counting kit-8 assays demonstrated that the LG fibroblasts in cGVHD were highly proliferative, and cell invasion assays indicated that they were highly invasive with high migration ability.

Conclusions:
LG fibroblasts in cGVHD can be aberrantly activated, thereby eliciting fibrosis by producing excessive extracellular matrix, leading to LG dysfunction in mice.
Gout Keratitis: A Case of Peripheral Ulcerative Keratitis Secondary to Gout With a Review of the Literature
Authors: Yazdanyar Amirfarbod , Rizzuti Allison E. , Mechel Elzbieta , Denisova Ksenia , Lazzaro Douglas R. 
Published: October 2017

Purpose:
To report a case of peripheral ulcerative keratitis secondary to gout.

Methods:
A 41-year-old man with a history of severe gout disease presented with pain and redness of the right eye. Physical examination revealed 2 areas of peripheral corneal thinning with overlying epithelial defects. Adjacent to these areas, reflective crystals were identified in the corneal stroma. Anterior segment optical coherence tomography demonstrated stromal corneal deposits.

Results:
Systemic workup was negative aside from an elevated serum uric acid level. The patient was administered oral prednisone, allopurinol, and colchicine. At his 2-month follow-up visit, the patient was asymptomatic and his corneal thinning had significantly improved.

Conclusions:
Gout is the most common type of inflammatory arthritis in adults with rising incidence and prevalence. Ocular findings in gout are common, but patients are usually asymptomatic. Monosodium urate crystal deposition has been reported to occur in various parts of the eye, with and without ocular inflammation. Crystal deposition in the cornea is extremely rare and may be a cause of peripheral ulcerative keratitis.
Early management of acute cerebrovascular accident
Authors: Manners Jody , Steinberg Alexis , Shutter Lori 
Published: October 2017

Purpose of review
Stroke is common and often presents as a neurologic emergency that requires rapid evaluation and treatment to minimize debilitation. Recent advances in therapy expanded time windows for intra-arterial thrombectomy in ischemic stroke, and surgical interventions for clot evacuation in large intracranial hemorrhage have recently proven feasible. This review discusses recent data regarding new therapeutic options in both ischemic and hemorrhagic stroke, notably in scenarios in which therapy was previously limited to supportive care.

Recent findings
Recent data show that intra-arterial therapy in ischemic stroke provides both benefit in outcomes and potential for further advancements in care. Therapeutic windows for endovascular treatment of a cerebral vessel occlusion now extend to 6 h, and recent data suggest this may increase further to 24 h. Intervention in hemorrhagic stroke remains limited to reversal of coagulopathy and hypertension; however, surgical techniques are underway and may prove beneficial in some cases.

Summary
Advancing therapeutics in ischemic and hemorrhagic stroke are changing acute care intervention and broadening potential candidates for what were once thought to be nonintervenable conditions. Execution of best practices in stroke will continue to evolve and will require understanding advanced imaging techniques, as well as selection criteria for procedural and surgical interventions.
Effect of Experimental Conditions in the Accommodation Response in Myopia
Authors: Otero Carles , Aldaba Mikel , Vera-Diaz Fuensanta A. , Pujol Jaume 
Published: October 2017

SIGNIFICANCE
The accommodative response is more affected by the type of refractive error than the method of stimulation, field of view (FOV), or stimulus depth.

PURPOSE
This study aims to analyze the effect of stimulation method, stimulus depth, and FOV on the accommodation response (AR) for emmetropes (EMM), late-onset myopes (LOM), and early-onset myopes (EOM).

METHODS
Monocular AR was measured in 26 young observers (n = 9 EMM, n = 8 LOM, n = 9 EOM) under 60 different viewing conditions that were the result of permuting the following factors: (1) stimulation method (free space or Badal lens viewing), (2) stimulus depth (flat or volumetric), (3) FOV (2.5, 4, 8, 10, and 30°), and (4) accommodative stimulus (AS: 0.17, 2.50, and 5.00 diopters [D]).

RESULTS
Mixed analysis of variance for 2.50 D of AS resulted in a significant effect of refractive group (F = 6.77, P < .01) and FOV (F = 1.26, P = .04). There was also a significant interaction between stimulus depth and FOV (F = 2.73, P = .03) and among stimulation method, FOV, and refractive group (F = 2.42, P = .02). For AS of 5.00 D, there was a significant effect of refractive group (F = 13.88, P < .01) and stimulation method (F = 5.16, P = .03). There was also a significant interaction of stimulation method, stimulus depth, and refractive group (F = 4.08, P = .03). When controlling for all interactions, LOM showed larger lags than EMM and EOM; the AR did not significantly change for fields of 8, 10, and 30°, and it did not significantly differ for different stimulation methods or stimulus depth.

CONCLUSIONS
Previously reported differences in AR when using lens-based methods compared with free space viewing may be explained by the effect of other factors such as the FOV or the depth of the stimulus. Targets with an FOV of 8 or 10° may be optimal for accurate ARs.
The Effect of Low-Frequency Repetitive Transcranial Magnetic Stimulation at Orbitofrontal Cortex in the Treatment of Patients With Medication-Refractory Obsessive-Compulsive Disorder
Authors: Kumar Saurabh , Singh Swarndeep , Chadda Rakesh Kumar , Verma Rohit , Kumar Nand 
Published: October 2017

Objective
Obsessive-compulsive disorder (OCD) is a chronic debilitating psychiatric disorder, with significant proportion of patients failing to respond with current first-line treatments. The present study assesses the safety and effectiveness of low-frequency repetitive transcranial magnetic stimulation (LF-rTMS) over left-orbitofrontal cortex (Lt-OFC) as a potential augmentation strategy in treatment of patients with medication-refractory OCD in real-world clinical setting. The present report also aims to examine the factors affecting response to rTMS and the durability of effects produced by rTMS over 1 month of follow-up period.

Methods
Retrospective review and analysis of clinical case files of 25 patients with medication-refractory OCD, all of whom had received 20 sessions of LF-rTMS over Lt-OFC as part of routine clinical care. A reduction of 25% and 35% in Yale-Brown Obsessive Compulsive Scale scores was used to determine the proportion of partial and complete responders, respectively.

Results
There was a significant decrease in mean Yale-Brown Obsessive Compulsive Scale score at the end of 20 sessions of rTMS compared with baseline (7.04 ± 5.07; P < 0.001), with no further significant change during the subsequent 1-month follow-up period (0.20 ± 1.38; P = 0.47). Thirteen patients (52%) met criteria for partial response, of which 11 patients (44%) showed complete response. Furthermore, higher number of failed medication trials was found to be significantly associated with greater chances of nonresponse to rTMS treatment.

Conclusions
There is a role of applying LF-rTMS over Lt-OFC as an augmentation strategy in ameliorating clinical symptoms among patients with medication-refractory OCD.
The representation of colored objects in macaque color patches
Authors: Chang Le , Bao Pinglei , Tsao Doris Y. 
Published: October 2017

AbstractAn important question about color vision is: how does the brain represent the color of an object? The recent discovery of “color patches” in macaque inferotemporal (IT) cortex, the part of brain responsible for object recognition, makes this problem experimentally tractable. Here we record neurons in three color patches, middle color patch CLC (central lateral color patch), and two anterior color patches ALC (anterior lateral color patch) and AMC (anterior medial color patch), while presenting images of objects systematically varied in hue. We found that all three patches contain high concentrations of hue-selective cells, and the three patches use distinct computational strategies to represent colored objects: while all three patches multiplex hue and shape information, shape-invariant hue information is much stronger in anterior color patches ALC/AMC than CLC; furthermore, hue and object shape specifically for primate faces/bodies are over-represented in AMC but not in the other two patches.
Predicting the genetic loci of past evolution
Authors: Courtier-Orgogozo Virginie , Martin Arnaud 
Published: October 2017

AbstractRepetitions in the mutations found to be responsible for independent evolution of similar phenotypes in various taxa have led some biologists to propose that for certain evolutionary changes the causal mutations are predictable. We examine here the nature of the predictions that have been made and their associated arguments. Predictions about the loci of past evolution are retrodictions, i.e. inferences about events that occurred in the past. They are not based on elaborate models and they derive mainly from the observation of repeated cases of genetic evolution. Predictions at the nucleotide level or at the gene level have a higher inference gain than those for broader categories of genetic changes such as cis-regulatory mutations.
The plant calcium-dependent protein kinase CPK3 phosphorylates REM1.3 to restrict viral infection
Authors: Perraki Artemis , Gronnier Julien , Gouguet Paul , Boudsocq Marie , Deroubaix Anne-Flore , Simon Vincent , German-Retana Sylvie , Zipfel Cyril , Bayer Emmanuelle , Mongrand Sébastien , Germain Véronique 
Published: October 2017

AbstractPlants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that theArabidopsis thalianaCALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINsin vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3-s phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.