bluuebunny/crossref_metadata_2025_split huggingface dataset:
A bromodomain-DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT
Authors: Miller Thomas C.R. , Simon Bernd , Rybin Vladimir , Grötsch Helga , Curtet Sandrine , Khochbin Saadi , Carlomagno Teresa , Müller Christoph W. 
Published: September 2016

AbstractBromodomains are critical components of many chromatin modifying/remodeling proteins and are emerging therapeutic targets, yet how they interact with nucleosomes, rather than acetylated peptides, remains unclear. Using BRDT as a model, we characterized how the BET family of bromodomains interacts with site-specifically acetylated nucleosomes. We find that BRDT interacts with nucleosomes through its first (BD1), but not second (BD2) bromodomain, and that acetylated histone recognition by BD1 is complemented by a novel bromodomain-DNA interaction. Simultaneous DNA and histone recognition enhances BRDT's nucleosome binding affinity, specificity, and ability to localize to and compact acetylated chromatin. Conservation of DNA binding in bromodomains of BRD2, BRD3 and BRD4, indicates that bivalent nucleosome recognition is a key feature of these bromodomains and possibly others. Our results elucidate the molecular mechanism of BRDT association with nucleosomes and identify new structural features of BET bromodomains that may be targeted for therapeutic inhibition.
PRIMA: a gene-centered, RNA-to-protein method for mapping RNA-protein interactions
Authors: Tamburino Alex M. , Kaymak Ebru , Shrestha Shaleen , Holdorf Amy D. , Ryder Sean P. , Walhout Albertha J.M. 
Published: September 2016

SUMMARYInteractions between RNA binding protein (RBP) and mRNAs are critical to post-transcriptional gene regulation. Eukaryotic genomes encode thousands of mRNAs and hundreds of RBPs. However, in contrast to interactions between transcription factors (TFs) and DNA, the interactome between RBPs and RNA has been explored for only a small number of proteins and RNAs. This is largely because the focus has been on using ‘protein-centered’ (RBP-to-RNA) interaction mapping methods that identify the RNAs with which an individual RBP interacts. While powerful, these methods cannot as of yet be applied to the entire RBPome. Moreover, it may be desirable for a researcher to identify the repertoire of RBPs that can interact with an mRNA of interest – in a ‘gene-centered’ manner, yet few such techniques are available. Here, we present Protein-RNA Interaction Mapping Assay (PRIMA) with which an RNA ‘bait’ can be tested versus multiple RBP ‘preys’ in a single experiment. PRIMA is a translation-based assay that examines interactions in the yeast cytoplasm, the cellular location of mRNA translation. We show that PRIMA can be used with small RNA elements, as well as with full-lengthCaenorhabditis elegans3′UTRs. PRIMA faithfully recapitulates numerous well-characterized RNA-RBP interactions and also identified novel interactions, some of which were confirmedin vivo. We envision that PRIMA will provide a complementary tool to expand the depth and scale with which the RNA-RBP interactome can be explored.
Consumers’ willingness to pay and drivers of motivation to consume omega-3 enriched mozzarella cheese
Authors: Vecchio Riccardo , Lombardi Alessia , Cembalo Luigi , Caracciolo Francesco , Cicia Gianni , Masucci Felicia , Di Francia Antonio 
Published: September 2016

Purpose
Consumer interest and willingness to pay (WTP) for omega-3 enriched water buffalo mozzarella cheese are evaluated through an in-store experimental auction. The purpose of this paper is to estimate individual WTP for enriched mozzarella cheese and related it to self-efficacy beliefs and outcome expectations of omega-3 consumption, following regulatory focus theory.

Design/methodology/approach
Data were collected in May-June 2015 from a sample of 150 consumers in Southern Italy. A structural equation modelling procedure was implemented.

Findings
The results show a significant role is played by prevention outcome expectations on consumer behaviour. While promotion outcome expectation constructs proved non-significant, self-identity correlates with prevention outcome expectations.

Research limitations/implications
There are several limitations that the authors are aware of regarding this study. First of all, since the authors rely on self-reported measures, optimistic bias might have affected participants’ responses (Weinstein, 1980). Second, results may be influenced by the choice of the specific information provided to consumers for the analysis; different claims and different information framings should be tested (LeBoeuf and Shafir, 2003).

Practical implications
Implications stemming from the results encourage the promotion of omega-3 enriched mozzarella cheese based on stimulating outcome expectations, bearing in mind that individual motivations should be enhanced by self-identity beliefs.

Originality/value
Although the combined role of self-efficacy and outcome expectations on personal intention to adopt healthy behaviour has already been demonstrated (Keller, 2006; Tudoran et al., 2012), to the best of the knowledge no previous study relates individual behaviour to an intention measured as a WTP for an actual product. In addition, current study has applied a non-hypothetical BDM (from Becker et al., 1964) auction in-store experiment.
Food, identity and belonging: a case study of South African-Australians
Authors: Schermuly Allegra Clare , Forbes-Mewett Helen 
Published: September 2016

Purpose
Culturally familiar food is of great importance to migrants. The purpose of this paper is to examine the role of food in the lives of South African migrants to Australia. How food impacts on notions of identity and belonging for immigrants is framed and discussed within the context of nostalgia, sharing and Bourdieu’s “habitus”.

Design/methodology/approach
Through mixed qualitative methods, including participatory research, document analysis and in-depth interviews, this study examines the everyday experiences of South African-Australians. The study employs an interpretivist approach that aims for greater understanding of the subject through the perspectives of the research participants.

Findings
Culinary rituals and traditions feature large in personal narratives of adjustment that reveal the important role of food in contributing to identity translation in a destination society and, ultimately, the attainment of belonging for migrants.

Research limitations/implications
The study provides a “snapshot” of a topic that would benefit from further exploration.

Practical implications
The importance for migrants to have access to cultural traditions surrounding food is acknowledged in the contemporary world where increasingly mobile populations need to maintain a sense of identity and feel a sense of belonging while integrating into host societies.

Social implications
Traditional cuisines are an integral part of the mechanisms by which migrants can better integrate leading to overall greater social cohesion.

Originality/value
The study contributes a new dimension to the body of literature pertaining to food access and security for culturally diverse groups in multicultural societies.
Purchase intentions for domestic food: a moderated TPB-explanation
Authors: Vabø Mette , Hansen Håvard 
Published: September 2016

Purpose
The purpose of this paper is to investigate consumers’ intention to buy domestic food applying the theory of planned behavior. Based on this framework, the authors investigate the moderating effects of consumer ethnocentrism and self-construal.

Design/methodology/approach
To test the conceptual model, a cross-sectional study from a random sample of Norwegian consumers was employed. A total of 501 consumers filled out the web-based survey. The data were analyzed by means of confirmatory factor analysis and multiple regression.

Findings
The results show that subjective norm and perceived behavioral control (PBC) both have positive significant effects on consumers’ intention to buy domestic food. Attitude also has a positive effect but is only significant on the ten percent level. The effect of subjective norm is reduced with increasing levels of ethnocentrism, and the effect of PBC is reduced when consumers are collectivistic rather than individualistic.

Originality/value
This study provides the food industry with useful information about which mechanisms underlie the consumers’ intention to buy domestic food. In addition the study provides useful insight into how different personality characteristics affect the consumers’ intentions.
Consumer perceptions of farmed fish
Authors: Reinders Machiel J. , Banovi´ Marija , Guerrero Lluis , Krystallis Athanasios 
Published: September 2016

Purpose
The purpose of this paper is to investigate possible cross-cultural consumer segments in the EU aquaculture market and provide direction and focus for marketing strategies for farmed fish products.

Design/methodology/approach
Selected psychographic constructs (i.e. category involvement, domain-specific innovativeness, subjective knowledge, suspicion of novelties and optimistic bias) are tested as segmentation basis with the objective of defining a number of cross-border consumer segments with distinctive and clear-cut profiles in terms of consumer perceptions towards farmed fish.

Findings
Based on the consumer psychographic profiles, three distinct segments are found: involved traditional, involved innovators and ambiguous indifferent, of which the first two constitute especially interesting targets for market positioning strategies for aquaculture products.

Practical implications
The results of the segmentation analysis opens new horizons in terms of positioning and differentiation of fish products from the aquaculture industry according to the most important potential market segments.

Originality/value
The current research brings insights into different pan-European consumer segments and their characteristics that allow for a corresponding differentiation strategy within the aquaculture industry. The fact that the segments tend to be uniform across all countries suggests a relatively homogeneous or converging European fish-related culture.
Implicit preference of sweets over fruit as a predictor of their actual consumption
Authors: Pavlović Maša , Žeželj Iris , Marinković Maša , Sučević Jelena 
Published: September 2016

Purpose
The purpose of this paper is to test if our eating behavior is determined not only by conscious evaluations of certain foods (explicit attitudes), but also by their automatic evaluations (implicit attitudes).

Design/methodology/approach
In two studies, the authors examined the predictive and incremental validity of these two types of attitudinal measures of eating behavior. Implicit attitudes were assessed with a standard implicit attitude test procedure (target categories were “sweets” and “fruit,” and attribute categories were “good” and “bad”); two explicit attitude measures were assessed: an explicit measure of preference for sweets over fruit and a semantic differential measure. The behavioral measure in Study 1 was the quantity of sweets consumed; in Study 2, it was a relative measure of sweets vs fruit consumption registered through a three-day diary.

Findings
The relatively low correlation between implicit and explicit attitude measures indicated that these measures at least partially tap into different processes. Implicit attitudes proved to be superior over explicit attitudes in predicting food consumption, especially for consumption registered via diary. This fact suggests that implicit attitudes are powerful drivers of long-term behavior.

Practical implications
The findings could be useful in tailoring interventions to promote healthier eating habits.

Originality/value
The research tested predictive power of implicit food-related attitudes. It compared the food consumption in laboratory and real-life settings. A new measure for daily food consumption was developed and it was calculated relative to recommended serving size.
Different strokes for different folks? The impact of heterogeneity in work characteristics and country contexts on work-life balance among the self-employed
Authors: Annink Anne , Den Dulk Laura , Amorós José Ernesto 
Published: September 2016

PurposeThe purpose of this paper is to explore the effect of self-employed work characteristics (consumer orientation, innovativeness, number of employees, motivation, and entrepreneurial phase) on work-life balance (WLB) satisfaction.Design/methodology/approachThe job demands and resources approach is applied to test whether self-employed work characteristics are evaluated as job demands or resources for WLB. The Global Entrepreneurship Data (2013) offer a unique opportunity to conduct multilevel analysis among a sample of self-employed workers in 51 countries (N=11,458). Besides work characteristics, this paper tests whether country context might explain variation in WLB among the self-employed.FindingsThe results of this study reveal that there is a negative relation between being exposed to excessive stress and running a consumer-oriented business and WLB. Being motivated out of opportunity is positively related to WLB. In addition, the results indicate that country context matters. A higher human development index and more gender equality are negatively related to WLB, possibly because of higher social expectations and personal responsibility. The ease of doing business in a country was positively related to the WLB of self-employed workers.Social implicationsFor some workers self-employment might be a way to combine work and responsibilities in other life domains, but this does not seem to be valid in all cases.Originality/valueThis paper contributes to current literature on the WLB of self-employed workers by showing how work characteristics can be evaluated as job demands or resources. Including work characteristics in future research might be a solution for acknowledging the heterogeneity among self-employed workers.
Experimental methods in entrepreneurship research: the status quo
Authors: Kraus Sascha , Meier Fabian , Niemand Thomas 
Published: September 2016

PurposeThe purpose of this paper is to evaluate the emerging field of experimental research on entrepreneurship to better understand its development and potential.Design/methodology/approachA systematic, evidence-based literature review was applied, resulting in a sample of 47 articles having used experiments in entrepreneurship research so far. The papers are analyzed according to their topic, methods, and research design, revealing insight into their limitations and prospective contributions.FindingsThe paper discusses the potential and disadvantages of experimental methods while arguing for experiments as the method of choice for answering causality questions. This study finds a persistent increase in experimental entrepreneurship research since its introduction in 1990.Research limitations/implicationsThe study provides research from the field of entrepreneurship with future directions, with potential research areas and an orientation for those interested in conducting experiments.Originality/valueExperiments are employed in a variety of research areas and have become more and more popular in the field of entrepreneurship. No study has analyzed the experimental studies in entrepreneurship. This paper contributes by providing an overview of the field, reflecting and discussing the outcomes while characterizing the methods employed.
Access to bank finance for UK SMEs in the wake of the recent financial crisis
Authors: Cowling Marc , Liu Weixi , Zhang Ning 
Published: September 2016

Purpose
The purpose of this paper is to investigate how entrepreneurs demand for external finance changed as the economy continued to be mired in its third and fourth years of the global financial crisis (GFC) and whether or not external finance has become more difficult to access as the recession progressed.

Design/methodology/approach
Using a large-scale survey data on over 30,000 UK small- and medium-sized enterprises between July 2011 and March 2013, the authors estimate a series of conditional probit models to empirically test the determinants of the supply of, and demand for external finance.

Findings
Older firms and those with a higher risk rating, and a record of financial delinquency, were more likely to have a demand for external finance. The opposite was true for women-led businesses and firms with positive profits. In general finance was more readily available to older firms post-GFC, but banks were very unwilling to advance money to firms with a high-risk rating or a record of any financial delinquency. It is estimated that a maximum of 42,000 smaller firms were denied credit, which was significantly lower than the peak of 119,000 during the financial crisis.

Originality/value
This paper provides timely evidence that adds to the general understanding of what really happens in the market for small business financing three to five years into an economic downturn and in the early post-GFC period, from both a demand and supply perspective. This will enable the authors to consider what the potential impacts of credit rationing on the small business sector are and also identify areas where government action might be appropriate.
On the emergence of leadership in new ventures
Authors: Baldegger Urs , Gast Johanna 
Published: September 2016

Purpose
The purpose of this paper is to explore the emergence and development of leadership within the context of new ventures.

Design/methodology/approach
A qualitative approach was conducted to analyze in-depth the circumstances under which leadership is emerging and evolving in new ventures. In doing so, 55 founder-CEOs from Austria, Liechtenstein and Switzerland were interviewed.

Findings
The findings suggest that during the development from new ventures to early growth ventures the founder-CEOs and their organizations experience three major transitions. First, the founder-CEOs’ leadership behavior tends to emerge and evolve alongside firm development from being more transformational in new ventures to more transactional in early growth ventures. Second, the decisive employee selection criteria change over time, and the initially important person-founder fit turns into a person-organization fit. Third, a transition from a rather external perspective of the founder-CEOs in the new venture stage to a more internally oriented perspective in the early stages of growth was observed.

Research limitations/implications
Although the findings advance research on leadership in new ventures, the limitations concerning potential recall biases and subjectivism have to be kept in mind.

Practical implications
In practice, the findings imply that the emergence and development of leadership in new ventures should be seen as a dynamic process.

Originality/value
This paper is one of the first to study in-depth the emergence and development of leadership in the context of new ventures.
Does one size fit all? A look at entrepreneurial motivation and entrepreneurial orientation in the informal economy of Tanzania
Authors: Eijdenberg Emiel L. 
Published: September 2016

Purpose
The purpose of this paper is to investigate the impact of entrepreneurial motivation and entrepreneurial orientation in Tanzania’s informal economy. The development of personal wealth is used to measure the performance of subsistence entrepreneurs in the mama lishe sector.

Design/methodology/approach
On the basis of a literature review and a qualitative pre-study involving 27 interviewees, a questionnaire was completed by 152 subsistence entrepreneurs who are known as the mama lishe. Subsequently, a correlation analysis, a factor analysis, and regression analyses were performed to test the hypotheses.

Findings
On the basis of a factor analysis, a distinction is made between the development of basic personal wealth and the development of advanced personal wealth. Entrepreneurial motivation and entrepreneurial orientation, as constructed by studies in western countries, have little effect on both forms of personal wealth. Instead, the owner’s age, as an indicator of entrepreneurial experience, is a relatively strong predictor for the development of personal wealth.

Originality/value
By examining the determinants of the performance of subsistence entrepreneurs in an African least developed country (LDC), this study shows that the western definitions of entrepreneurial motivation and entrepreneurial orientation are not directly applicable in this context. By using performance measures such as the development of personal wealth, this study supports the perspective that entrepreneurs in the informal economy of an LDC operate under a different paradigm than their counterparts in the industrialized nations of the west. Accordingly, it is concluded that one size does not fit all.
A study of experimental and theoretical analysis of N-cyclohexylmethacrylamide monomer based on DFT and HF computations
Authors: Akman Feride , Çankaya Nevin 
Published: September 2016

PurposeThis paper aims to synthesise and characterise N-cyclohexylmethacrylamide (NCMA) monomer which contains thermosensitive group. The characterisation of monomer was performed both theoretically and experimentally.Design/methodology/approachThe monomer was prepared by reacting cyclohexylamine with methacryloyl chloride in the presence of triethylamine at room temperature. The synthesised monomer was characterised by using not only Density Functional Theory (DFT) and Hartree–Fock (HF) with the Gaussian 09 software but also fourier transform infrared (FT–IR),1H and13C nuclear magnetic resonance (NMR) spectroscopy.FindingsBoth the experimental and the theoretical methods demonstrated that the monomer was successfully synthesised. The vibrational frequencies, the molecular structural geometry, such as optimised geometric bond angles, bond lengths and the Mulliken atomic charges of NCMA were investigated by using DFT/B3LYP and HF methods with the 3-21G* basis set. The experimental results were compared with theoretical values. The results revealed that the calculated frequencies were in good accord with the experimental values. Besides, frontier molecular orbitals (FMOs) and molecular electrostatic potential of NCMA were investigated by theoretical calculations at the B3LYP/3–21G* basis set.Research limitations/implicationsMonomer and polymer containing a thermosensitive functional group have attracted great interest from both industrial and academic fields. Their characterisation can provide great opportunities for polymer science by using DFT and HF methods.Originality/valueThe monomer containing a thermosensitive functional group and a various polymer may be prepared by using DFT and HF methods described in this paper. The calculated data are greatly important to provide insight into molecular analysis and then used in technological applications.