bluuebunny/crossref_metadata_2025_split huggingface dataset:
One‐Pot Hybrid SnO2/Poly(methyl methacrylate) Nanocomposite Formation through Pulsed Laser Irradiation
Authors: Caputo Gianvito , Scarpellini Alice , Palazon Francisco , Athanassiou Athanassia , Fragouli Despina 
Published: April 2017

AbstractThe localized in situ formation of tin dioxide (SnO2) nanoparticles embedded in poly(methyl methacrylate) (PMMA) films is presented. This is achieved by the photoinduced conversion of the tin acetate precursor included in polymeric films, through controlled UV or visible pulsed laser irradiation at λ=355 and 532 nm, respectively. The evolution of the formation of nanoparticles is followed by UV/Vis spectroscopy and shows that their growth is affected in different ways by the laser pulses at the two applied wavelengths. This, in combination with electron microscopy analysis, reveals that, depending on the irradiation wavelength, the size of the nanoparticles in the final nanocomposites differs. This difference is attributed to distinct mechanistic pathways that lead to the synthesis of small nanoparticles (from 1.5 to 4.5 nm) at λ=355 nm, whereas bigger ones (from 5 to 16 nm) are formed at λ=532 nm. At the same time, structural studies with both X‐ray and electron diffraction measurements demonstrate the crystallinity of SnO2 nanoparticles in both cases, whereas XPS analysis confirms the light‐induced oxidation of tin acetate into SnO2. Taken all together, it is demonstrated that the pulsed laser irradiation at λ=355 and 532 nm leads to the formation of SnO2 nanoparticles with defined features highly dispersed in PMMA solid matrices.
Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase–Based Two‐Hybrid (BACTH) Technique
Authors: Ouellette Scot P. , Karimova Gouzel , Davi Marilyne , Ladant Daniel 
Published: April 2017

AbstractThe bacterial two‐hybrid (BACTH, for “Bacterial Adenylate Cyclase‐based Two‐Hybrid”) technique is a simple and fast genetic approach to analyze protein‐protein interactions in vivo. In this system, the proteins of interest are genetically fused to two complementary fragments from the catalytic domain of Bordetella pertussis adenylate cyclase and co‐expressed in strains of Escherichia coli deficient in adenylate cyclase. Association of the hybrid proteins restores synthesis of cyclic AMP (cAMP), which then triggers the expression of catabolic operons such as the lactose operon or the maltose regulon. As BACTH uses a cAMP second messenger, the association between the chimeric proteins can take place at a distance from the transcription machinery. This technique is therefore particularly appropriate for studying interactions involving integral‐membrane or membrane‐associated proteins that may not be soluble in the cytoplasm, and/or that may only associate in the plane of the membrane. This unit describes the basic procedures to characterize protein‐protein interactions with the BACTH genetic system and to search for potential partners of known proteins. © 2017 by John Wiley & Sons, Inc.
Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane‐Bound Protein Clusters
Authors: Rees Johanna Susan , Li Xue‐Wen , Perrett Sarah , Lilley Kathryn Susan , Jackson Antony Philip 
Published: April 2017

AbstractThis manuscript describes a new and general method to identify proteins localized into spatially restricted membrane microenvironments. Horseradish peroxidase (HRP) is brought into contact with a target protein by being covalently linked to a primary or secondary antibody, an antigen or substrate, a drug, or a toxin. A biotinylated tyramide‐based reagent is then added. In the presence of HRP and hydrogen peroxide, the reagent is converted into a free radical that only diffuses a short distance before covalently labeling proteins within a few tens to hundreds of nanometers from the target. The biotinylated proteins can then be isolated by standard affinity chromatography and identified by liquid chromatography (LC) and mass spectrometry (MS). The assay can be made quantitative by using stable isotope labeling with amino acids in cell culture (SILAC) or isobaric tagging at the peptide level. © 2017 by John Wiley & Sons, Inc.
Atomically Altered Hematite for Highly Efficient Perovskite Tandem Water‐Splitting Devices
Authors: Gurudayal  , John Rohit Abraham , Boix Pablo P. , Yi Chenyi , Shi Chen , Scott M. C. , Veldhuis Sjoerd A. , Minor Andrew M. , Zakeeruddin Shaik M. , Wong Lydia Helena , Grätzel Michael , Mathews Nripan 
Published: April 2017

AbstractPhotoelectrochemical (PEC) cells are attractive for storing solar energy in chemical bonds through cleaving of water into oxygen and hydrogen. Although hematite (α‐Fe2O3) is a promising photoanode material owing to its chemical stability, suitable band gap, low cost, and environmental friendliness, its performance is limited by short carrier lifetimes, poor conductivity, and sluggish kinetics leading to low (solar‐to‐hydrogen) STH efficiency. Herein, we combine solution‐based hydrothermal growth and a post‐growth surface exposure through atomic layer deposition (ALD) to show a dramatic enhancement of the efficiency for water photolysis. These modified photoanodes show a high photocurrent of 3.12 mA cm−2 at 1.23 V versus RHE, (>5 times higher than Fe2O3) and a plateau photocurrent of 4.5 mA cm−2 at 1.5 V versus RHE. We demonstrate that these photoanodes in tandem with a CH3NH3PbI3 perovskite solar cell achieves overall unassisted water splitting with an STH conversion efficiency of 3.4 %, constituting a new benchmark for hematite‐based tandem systems.
Modeled hydrologic metrics show links between hydrology and the functional composition of stream assemblages
Authors: Patrick Christopher J. , Yuan Lester L. 
Published: April 2017

AbstractFlow alteration is widespread in streams, but current understanding of the effects of differences in flow characteristics on stream biological communities is incomplete. We tested hypotheses about the effect of variation in hydrology on stream communities by using generalized additive models to relate watershed information to the values of different flow metrics at gauged sites. Flow models accounted for 54–80% of the spatial variation in flow metric values among gauged sites. We then used these models to predict flow metrics in 842 ungauged stream sites in the mid‐Atlantic United States that were sampled for fish, macroinvertebrates, and environmental covariates. Fish and macroinvertebrate assemblages were characterized in terms of a suite of metrics that quantified aspects of community composition, diversity, and functional traits that were expected to be associated with differences in flow characteristics. We related modeled flow metrics to biological metrics in a series of stressor–response models. Our analyses identified both drying and base flow instability as explaining 30–50% of the observed variability in fish and invertebrate community composition. Variations in community composition were related to variations in the prevalence of dispersal traits in invertebrates and trophic guilds in fish. The results demonstrate that we can use statistical models to predict hydrologic conditions at bioassessment sites, which, in turn, we can use to estimate relationships between flow conditions and biological characteristics. This analysis provides an approach to quantify the effects of spatial variation in flow metrics using readily available biomonitoring data.
Validation of the German fear of pain questionnaire in a sample of children with mixed chronic pain conditions
Authors: Flack F. , Gerlach A.L. , Simons L.E. , Zernikow B. , Hechler T. 
Published: April 2017

AbstractBackgroundTo date, no German instrument exists to measure pain‐related fear in paediatric pain populations. The objective of the current study was to determine the construct validity of the translated German fear of pain questionnaire for children (GFOPQ‐C) in a sample of children with mixed chronic pain disorders by testing the underlying factor structure, and its psychometric properties.MethodN = 241 children with mixed chronic pain disorders (aged 8–19 years) presenting to a specialized pain clinic completed the GFOPQ‐C and several other pain, fear and disability measures.ResultsThe two‐factor structure of the FOPQ‐C (fear, avoidance) was replicated. Internal consistency for the shortened German version was good for both subscales (Fear subscale: α = 0.89; avoidance subscale: α = 0.76). As expected, the fear subscale correlated highly with anxiety sensitivity (r = 0.63), pain catastrophizing (r = 0.62) and general anxiety (r = 0.54), while the avoidance subscale was more closely related to disability (r = 0.24) and school functioning (r = 0.28). Pain‐related fear differed in children with chronic pain depending on their pain location with higher fear ratings in children with abdominal pain and musculoskeletal pain.ConclusionThe GFOPQ‐C is a valid instrument that assesses two distinct dimensions of pain‐related fear in children: fear and avoidance. Future research is needed to evaluate the impact of increased pain‐related fear on outcomes over time as well as to examine pain‐related fear among healthy children. This will enhance our knowledge of who might be particularly vulnerable to potentially dysfunctional trajectories, such as ongoing pain or anxiety symptoms.SignificanceThe current study validates the first tool to assess pain‐related fear in German‐speaking children with chronic pain. Findings support two distinct domains: fear and activity avoidance.
A genome‐wide linkage and association analysis of imputed insertions and deletions with cardiometabolic phenotypes in Mexican Americans: The Insulin Resistance Atherosclerosis Family Study
Authors: Gao Chuan , Hsu Fang‐Chi , Dimitrov Latchezar M. , Okut Hayrettin , Chen Yii‐Der I. , Taylor Kent D. , Rotter Jerome I. , Langefeld Carl D. , Bowden Donald W. , Palmer Nicholette D. 
Published: April 2017

ABSTRACTInsertions and deletions (INDELs) represent a significant fraction of interindividual variation in the human genome yet their contribution to phenotypes is poorly understood. To confirm the quality of imputed INDELs and investigate their roles in mediating cardiometabolic phenotypes, genome‐wide association and linkage analyses were performed for 15 phenotypes with 1,273,952 imputed INDELs in 1,024 Mexican‐origin Americans. Imputation quality was validated using whole exome sequencing with an average kappa of 0.93 in common INDELs (minor allele frequencies [MAFs] ≥ 5%). Association analysis revealed one genome‐wide significant association signal for the cholesterylester transfer protein gene (CETP) with high‐density lipoprotein levels (rs36229491, P = 3.06 × 10−12); linkage analysis identified two peaks with logarithm of the odds (LOD) > 5 (rs60560566, LOD = 5.36 with insulin sensitivity (SI) and rs5825825, LOD = 5.11 with adiponectin levels). Suggestive overlapping signals between linkage and association were observed: rs59849892 in the WSC domain containing 2 gene (WSCD2) was associated and nominally linked with SI (P = 1.17 × 10−7, LOD = 1.99). This gene has been implicated in glucose metabolism in human islet cell expression studies. In addition, rs201606363 was linked and nominally associated with low‐density lipoprotein (P = 4.73 × 10−4, LOD = 3.67), apolipoprotein B (P = 1.39 × 10−3, LOD = 4.64), and total cholesterol (P = 1.35 × 10−2, LOD = 3.80) levels. rs201606363 is an intronic variant of the UBE2F‐SCLY (where UBE2F is ubiquitin‐conjugating enzyme E2F and SCLY is selenocysteine lyase) fusion gene that may regulate cholesterol through selenium metabolism. In conclusion, these results confirm the feasibility of imputing INDELs from array‐based single nucleotide polymorphism (SNP) genotypes. Analysis of these variants using association and linkage replicated previously identified SNP signals and identified multiple novel INDEL signals. These results support the inclusion of INDELs into genetic studies to more fully interrogate the spectrum of genetic variation.
Management of unilateral recurrent laryngeal nerve injury after thyroid surgery: A review
Authors: Lynch Jeremy , Parameswaran Rajeev 
Published: April 2017

AbstractBackgroundRecurrent laryngeal nerve (RLN) damage because of thyroid and parathyroid surgery has been recognized for over a century. Injury rates have been slowly decreasing in this period while effective treatment strategies have been increasing.MethodsRecent literature was evaluated on the topics of anatomy, pathophysiology, avoidance, and conservative and surgical treatment of RLN injury. Data for this literature review were identified by PubMed and references from relevant articles using the search terms “thyroid,” “laryngeal nerve,” and “injury.” Only articles published in English between 1990 and 2015 were included.ResultsAdvances in technique and equipment have made injury less likely. The evidence and role for neuromonitoring is discussed. Treatment strategies may include speech therapy, vocal cord augmentation using injection, laryngeal framework surgery techniques (including laryngoplasty and arytenoid adduction), and reinnervation.ConclusionInjury rates in specialist centers are very low. Good to excellent results may be obtained in most cases.
Sinonasal adenoid cystic carcinoma: Treatment outcomes and association with human papillomavirus
Authors: Miller Eric D. , Blakaj Dukagjin M. , Swanson Benjamin J. , Xiao Weihong , Gillison Maura L. , Wei Lai , Bhatt Aashish D. , Diavolitsis Virginia M. , Wobb Jessica L. , Kang Stephen Y. , Carrau Ricardo L. , Grecula John C. 
Published: April 2017

AbstractBackgroundThe purpose of this study was to review long‐term outcomes of sinonasal adenoid cystic carcinoma (ACC) and to clarify its association with human papillomavirus (HPV).MethodsThe medical records of 23 patients with sinonasal ACC treated with primary surgical resection between 1998 and 2013 were reviewed. Tissue specimens were available for 17 patients. The p16 testing was performed using immunohistochemistry (IHC), and HPV infection was determined using quantitative polymerase chain reaction (PCR) with primers targeting the E6/E7 region.ResultsTwo of the 17 samples showed strong and diffuse p16 staining, whereas the remaining 15 cases showed p16‐positivity isolated to the luminal cells. Only one of the p16‐positive cases was positive for HPV. The 5‐year local failure, disease‐free survival (DFS), and overall survival (OS) were 51%, 52%, and 62%, respectively.ConclusionLocal failures are common with advanced sinonasal ACC, and the association of HPV with true sinonasal ACC is low.
Chitosan conduits filled with simvastatin/Pluronic F‐127 hydrogel promote peripheral nerve regeneration in rats
Authors: Guo Qi , Liu Can , Hai Bao , Ma Teng , Zhang Wen , Tan Jie , Fu Xin , Wang Hong , Xu Yingsheng , Song Chunli 
Published: April 2017

AbstractTreating peripheral nerve defects represents a clinical challenge, and nerve conduits lacking an internal scaffold lead to limited large nerve gap regeneration. Here, we bridged 10‐mm sciatic nerve defects in rats with a chitosan conduit filled with 0, 0.5, or 1.0 mg of simvastatin in Pluronic F‐127 hydrogel. We assessed subsequent nerve regeneration using the sciatic functional index (SFI), electrophysiological assessments, Fluoro‐Gold (FG) retrograde tracing, gastrocnemius muscle mass measurements, and histological and immunohistochemical assessments of nerve regeneration. Ten weeks after implantation, the chitosan conduit filled with simvastatin/Pluronic F‐127 hydrogel promoted nerve regeneration; there were significant increases in the SFI, compound muscle action potential peak amplitude, motor nerve conduction velocity, FG‐labeled neuron number in the dorsal root ganglia, myelin sheath thickness, axon diameter, gastrocnemius wet weight, and muscle fiber area percentage in the gastrocnemius muscle (all p < 0.05). The expression levels of neurotrophic factors, such as pleiotrophin, hepatocyte growth factor, vascular endothelial growth factor, and glial cell line‐derived neurotrophic factor, were also found to be increased. The results suggest that chitosan conduits filled with simvastatin/Pluronic F‐127 hydrogel improved peripheral nerve regeneration and functional recovery in rats, which may have been related to the increased expression of several endogenous neurotrophic factors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 787–799, 2018.
Value of prior imaging review before ordering renal sonography in the evaluation of abnormal renal function
Authors: Niesen Timothy J. , Finch Nathan , Gakhal Mandip , Dressler Robert M. , Levy Howard M. 
Published: April 2017

ABSTRACTBackgroundTo determine the prognostic relevance of prior imaging studies in the evaluation of patients referred for renal ultrasound (US) examination to investigate abnormal renal function tests.MethodsWe conducted a retrospective study of 208 consecutive renal US examinations performed for abnormal renal function tests.Results68% (142/208) of patients reviewed for the study had prior abdominal imaging with 15% (21/142) receiving that imaging within 1 month prior to the renal US study and 56% (80/142) within the prior year. Of all patients with prior imaging studies, only 6/142 (4%) demonstrated any significant interval change, with development of hydronephrosis, which was also clinically evident as a substantial rise in serum creatinine level.ConclusionsReview of prior imaging studies, in addition to other pertinent clinical data, should result in a significant reduction in the number of unnecessary renal US examinations performed in patients with abnormal renal function. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 45:537–541, 2017
Nongalenic pial arteriovenous fistula: Prenatal diagnosis
Authors: Martínez‐Payo Cristina , Sancho Saúco Javier , Miralles Maria , Pérez Medina Tirso 
Published: April 2017

ABSTRACTPial arteriovenous (AV) fistulae have rarely been diagnosed in utero. They are characterized by one or more pial arteries flowing directly into a cortical vein without any shunt or interposed capillary bed. In the fetus and the newborn up to 2 years of age, the most common clinical manifestation is heart failure resulting from fistula overload. Later on, hydrocephalus, focal neurologic deficits, headaches, seizures, and cerebral hemorrhage are the most common manifestations. We present a case of nongalenic pial AV fistula diagnosed in the 25th week of pregnancy, which resulted in intrauterine fetal death due to congestive heart failure. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 45:621–625, 2017
Preoperative MRI features associated with lymphovascular invasion in node‐negative invasive breast cancer: A propensity‐matched analysis
Authors: Cheon Hyejin , Kim Hye Jung , Lee So Mi , Cho Seung Hyun , Shin Kyung Min , Kim Gab Chul , Park Ji Young , Kim Won Hwa 
Published: April 2017

PurposeIn node‐negative disease, the presence of lymphovascular invasion (LVI) is reported to be an unfavorable prognostic factor. Thus, the aim of this study was to evaluate whether preoperative breast MRI features are associated with LVI in patients with node‐negative invasive breast cancer by a propensity‐matched analysis.Materials and MethodsAmong 389 patients with node‐negative invasive ductal breast cancer who had preoperative breast 3.0 Tesla MRI with precontrast T2‐weighted fat‐suppressed, pre‐ and dynamic postcontrast T1‐weighted fat‐suppressed sequences, 61 patients with LVI (LVI group) were matched with 183 patients without LVI (no LVI group) at a ratio of 1:3 in terms of age, histologic grade, tumor size, and hormone receptor status. Two radiologists reviewed the MRI features, following profiles of focal breast edema (peritumoral, prepectoral, subcutaneous), intratumoral T2 signal intensity, adjacent vessel sign, and increased ipsilateral whole‐breast vascularity, in addition to 2013 Breast Imaging Reporting and Data System lexicon.ResultsThe presence of peritumoral edema (45.9% [28/61] versus 30.6% [56/183], P = 0.030) and adjacent vessel sign (82.0% [50/61] versus 68.3% [125/183], P = 0.041) was significantly associated with LVI. Prepectoral edema was also more frequently observed in the LVI group than in the no LVI group with borderline significance (26.2% [16/61] versus 15.3% [28/183], P = 0.055). In cases of nonmass enhancement, regional enhancement was more frequently found in the LVI group than in the no LVI group (60.0% [3/4] versus 5.9% [1/4], P = 0.042).ConclusionPreoperative breast MRI features may be associated with LVI in patients with node‐negative invasive breast cancer.Level of Evidence: 3Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2017;46:1037–1044.
Impact of changes in mean and extreme temperatures caused by climate change on olive flowering in southern Spain
Authors: Gabaldón‐Leal Clara , Ruiz‐Ramos Margarita , de la Rosa Raúl , León Lorenzo , Belaj Angjelina , Rodríguez Alfredo , Santos Cristina , Lorite Ignacio J. 
Published: April 2017

ABSTRACTDue to the severe increase projected in future temperatures and the great economic and social importance of olive growing for vast agricultural areas in the Mediterranean Basin, accurate climate change impact assessment on olive orchards is required. The aim of this study is to assess the flowering date and the impact of mean and extreme temperature events on olive flowering in southern Spain under baseline and future climate conditions. To that end, experimental data were obtained from ten olive genotypes: six well‐known olive cultivars in the region, one cultivar, ‘Chiquitita’, obtained via conventional breeding, and three wild olives from the Canary Islands. A site‐specific model calibration was conducted resulting in satisfactory performance with an average error of 2 days for flowering date estimation under baseline and future climate conditions, and a RMSE equal to 5.5 days in the validation process. The outputs from 12 regional climate models from the ENSEMBLES European project with a bias correction in temperature and precipitation were used. Results showed an advance in the olive flowering dates of about 17 days at the end of the 21st century compared with the baseline period (1981–2010), and an increase in the frequency of extreme events around the flowering period. A spatial analysis of results identified the areas in southern Spain that are most vulnerable to climate change impact caused by the lack of chilling hours accumulation (areas located on the Atlantic coast and the south‐eastern coast) and by the occurrence of high temperatures during the flowering period (areas located in the north and north‐eastern areas of the Andalusian region).
Adsorption Studies of Amino Cellulose on Cellulosics
Authors: Jedvert Kerstin , Elschner Thomas , Heinze Thomas 
Published: April 2017

Adsorption of a typical example of a new class of amino cellulose, namely 6‐deoxy‐6‐(2‐aminoethyl)amino cellulose at different pH‐values and in the presence of electrolytes, onto cellulose model substrates is studied with surface plasmon resonance and quartz crystal microbalance with dissipation monitoring. Unexpectedly, adsorption is consistently higher at a higher pH‐value of 10, indicating that solubility and interactions between amine moieties and cellulose are more important than electrostatic interactions. The findings are highly relevant for the process to modify material surfaces with amino cellulose in water‐based systems as a universal tool for changing the surface properties and chemistry. Potential applications for an antimicrobial all biobased material could be found, e.g., as medical textiles or in the biotechnology sector.
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