bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plant Growth Promoting Rhizobacteria (PGPR) induced protection: A plant immunity perspective
Authors: Kumari Rinkee , Pandey Ekta , Bushra Sayyada , Faizan Shahla , Pandey Saurabh 
Published: September 2024

AbstractPlant‐environment interactions, particularly biotic stress, are increasingly essential for global food security due to crop losses in the dynamic environment. Therefore, understanding plant responses to biotic stress is vital to mitigate damage. Beneficial microorganisms and their association with plants can reduce the damage associated with plant pathogens. One such group is PGPR (Plant growth‐promoting rhizobacteria), which influences plant immunity significantly by interacting with biotic stress factors and plant signalling compounds. This review explores the types, metabolism, and mechanisms of action of PGPR, including their enzyme pathways and the signalling compounds secreted by PGPR that modulate gene and protein expression during plant defence. Furthermore, the review will delve into the crosstalk between PGPR and other plant growth regulators and signalling compounds, elucidating the physiological, biochemical, and molecular insights into PGPR's impact on plants under multiple biotic stresses, including interactions with fungi, bacteria, and viruses. Overall, the review comprehensively adds to our knowledge about PGPR's role in plant immunity and its application for agricultural resilience and food security.
Which Novel Ingredient Should be Considered the “Holy Grail” for Sustainable Production of Finfish Aquafeeds?
Authors: Panteli Nikolas , Kousoulaki Katerina , Antonopoulou Efthimia , Carter Chris G. , Nengas Ioannis , Henry Morgane , Karapanagiotidis Ioannis T. , Mente Elena 
Published: September 2024

ABSTRACTThe immense production of fishmeal and fish oil is dramatically intensifying the severe state of pelagic fisheries overexploitation. The latter in conjunction with the increasing demand for low‐cost protein‐rich food supply prompt aquaculture to employ new practice. Several novel dietary ingredients are currently under evaluation for potential incorporation in aquafeeds in an effort to shift the aquaculture sector toward a more sustainable and economic production. The present review aims to summarize the existing findings regarding the effects of studied alternatives to fishmeal and fish oil on the most valuable and commercially produced marine (Sparus aurata and Dicentrarchus labrax) and freshwater (Salmo salar and Oncorhynchus mykiss) finfish species in European aquaculture. Alternative protein sources, including macroalage (marine plants), krill (marine fishery), insects (terrestrial), terrestrial animal by‐products (processed/rendered), and single cell ingredient (biotechnology), are discussed for their efficiency in promoting the growth and the welfare of both fry and adult cultured finfish species. Applicability of these ingredients is reviewed in terms of nutrient composition, dietary inclusion level, performance output, digestibility, and health benefits. In addition, a meta‐analysis was conducted based on data from peer‐reviewed scientific publications in order to assess whether novel ingredients meet the dietary protein (amino acid) and lipid requirements of finfishes. Aquafeed reformulation strategies should ensure the recommended daily nutritional requirements and additionally indicate the meta‐analysis alternatives, such as microalgae, which are deficient in essential amino acids. The sustainable expansion of aquaculture is on the horizon, but which novel ingredients may be regarded as the key drivers to its establishment?
Ambiguous and Complex – Rural Revival in a Peripheral Mountain Region (SW Poland)
Authors: Latocha‐Wites Agnieszka , Kajdanek Katarzyna , Sikorski Dominik , Szmytkie Robert , Tomczak Przemysław 
Published: September 2024

AbstractThe general development conditions of rural areas have been addressed by many authors; however, the conditions and factors that allow the depopulated problem rural areas to re‐enter the development path are an understudied phenomenon. This article examines the types and drivers of rural revival, evaluates the transformations of rural areas and forecasts their future development. It focuses on a representative region for the key transformations of mountainous rural areas in the Polish‐Czech borderland. This study offers a complex, interdisciplinary approach using quantitative and qualitative methods to examine the in‐depth mechanisms of rural transformation and to vocalise local knowledge and inhabitants' perceptions. The results demonstrate that the depopulated and marginalised peripheral rural regions have the potential to return to the development path, but the symptoms, drivers and perceptions of rural transformations vary greatly across the region. The study highlights the complexity and ambiguity of assessing, evaluating and forecasting rural transformations.
Survey of pediatric massive transfusion protocol practice at United States level I trauma centers: An AABB Pediatric Transfusion Medicine Subsection study
Authors: Adkins Brian D. , Noland Daniel K. , Jacobs Jeremy W. , Booth Garrett S. , Malicki Denise , Helander Louise , Jacquot Cyril , Buscema Gina , Goel Ruchika , Andrews Jennifer , Lieberman Lani 
Published: September 2024

AbstractBackgroundTrauma remains the leading cause of pediatric mortality in the United States. Although use of massive transfusion protocols (MTPs) in this population is widespread, optimal pediatric resuscitation is not well established. We sought to assess contemporary pediatric MTP practice in the United States.Study Design and MethodsA web‐based survey was designed by the Association for the Advancement of Blood & Biotherapies (AABB) Pediatric Transfusion Medicine Subsection and distributed to select American College of Surgeons (ACS) Level I Verified pediatric trauma centers. The survey assessed current MTP policy, implementation, and recent changes in practice.ResultsResponse rate was 55% (22/40). Almost half of the respondents were from the South. The median RBC:plasma ratio was 1 (interquartile range 1–1.5). Protocolized fibrinogen supplementation was common while integration of antifibrinolytic therapy into MTPs was infrequent. Viscoelastic testing (VET) was available at most sites, 71% (15/21, one site did not respond), and was generally utilized on an ad‐hoc basis. Roughly, a third of sites had changed their MTP in the past 3 years due to blood supply issues, and about a third reported having group O Whole Blood on‐site.ConclusionMTP practice is similar throughout the United States. Though fibrinogen supplementation is common—other emerging interventions such as antifibrinolytic therapy or utilization of routine viscoelastic testing—are not widespread. Pediatric transfusion medicine experts must continue to follow practice change, as contemporary large trials begin to characterize new supportive modalities to optimize resuscitation in pediatric trauma patients.
Characterization of blood bank and transfusion medicine practices for pregnant individuals with fetuses at risk of hemolytic disease in the United States
Authors: Jacobs Jeremy W. , Booth Garrett S. , Moise Kenneth J. , Adkins Brian D. , Bakhtary Sara , Fasano Ross M. , Goel Ruchika , Hinton Hannah D. , Laghari Sadia A. , Stephens Laura D. , Tormey Christopher A. , Crowe Elizabeth P. , Bloch Evan M. , Abels Elizabeth A. 
Published: September 2024

AbstractBackgroundHemolytic disease of the fetus and newborn (HDFN) is caused by maternal alloantibody‐mediated destruction of fetal/neonatal red blood cells (RBCs). While the pathophysiology has been well‐characterized, the clinical and laboratory monitoring practices are inconsistent.MethodsWe surveyed 103 US institutions to characterize laboratory testing practices for individuals with fetuses at risk of HDFN. Questions included antibody testing and titration methodologies, the use of critical titers, paternal and cell‐free fetal DNA testing, and result reporting and documentation practices.ResultsThe response rate was 44% (45/103). Most respondents (96%, 43/45) assess maternal antibody titers, primarily using conventional tube‐based methods only (79%, 34/43). Among respondents, 51% (23/45) rescreen all individuals for antibodies in the third trimester, and 60% (27/45) perform paternal RBC antigen testing. A minority (27%, 12/45) utilize cell‐free fetal DNA (cffDNA) testing to predict fetal antigen status. Maternal antibody titers are performed even when the fetus is not considered to be at risk of HDFN based on cffDNA or paternal RBC antigen testing at 23% (10/43) of sites that assess titers.DiscussionThere is heterogeneity across US institutions regarding the testing, monitoring, and reporting practices for pregnant individuals with fetuses at risk of HDFN, including the use of antibody titers in screening and monitoring programs, the use of paternal RBC antigen testing and cffDNA, and documentation of fetal antigen results. Standardization of laboratory testing protocols and closer collaboration between the blood bank and transfusion medicine service and the obstetric/maternal‐fetal medicine service are needed.
Optimal Control of a Roll-to-Roll Dry Transfer Process With Bounded Dynamics Convexification
Authors: Martin Christopher , Zhao Qishen , Bakshi Soovadeep , Velasquez Enrique , Li Wei , Chen Dongmei 
Published: September 2024

Abstract
For efficient roll-to-roll (R2R) production of flexible electronic components, a precise R2R transfer peeling process is essential, requiring accurate modeling and control. This paper introduces a novel approach to confining the dynamics of a nonlinear R2R mechanical peeling system within a convex set known as a norm-bounded linear differential inclusion (NLDI). This method utilizes constraints on uncertain system variables to create a tighter NLDI representation compared to other convexification techniques. Moreover, it offers drastically reduced computational cost compared to previous methods applied to convexify the R2R peeling system. The NLDI is employed to generate an H∞-optimal controller for the R2R peeling system, and both simulations and experiments demonstrate better dynamic performance compared to other controllers for R2R transfer.
Design, Modeling, and Control of a Hardware-in-the-Loop Testbed for Off-Road Vehicles
Authors: Zhao Gaonan , Yao Jie , Edson Connor P. , Sun Zongxuan 
Published: September 2024

Abstract
This paper presents the design, modeling, and control of a hardware-in-the-loop (HIL) testbed for off-road vehicles. The proposed HIL testbed employs a transient hydrostatic dynamometer to load a diesel engine to emulate any loading cycles of a wheel loader, which is a representative off-road vehicle. A fully validated wheel loader model is used to calculate the engine load, including both the drive and work functions. Besides, iterative learning control (ILC) has been designed for the loading torque tracking of the hydrostatic dynamometer to ensure accurate emulation of real-world operation scenarios. The developed HIL testbed is used to demonstrate more than 26% energy benefits of automated wheel loaders through systematic optimization compared with human-operated wheel loaders. This HIL testbed serves as a robust platform for advancing research and development across various off-road vehicles, including excavators, tractors, and harvesters.
Comprehensive Analysis of Critical Heat Flux and Heat Transfers in a Confined Jet Impingement Boiling Cooling System
Authors: Guerfi Djamel Eddine , Roux Stéphane , Allanic Nadine , Sarda Alain , Lecointe Damien 
Published: September 2024

Abstract
This study deepens the understanding of the heterogeneity of thermal exchanges in a cylindrical channel cooled by impinging water jets, considering the influence of transverse air flow and gravity. The results reveal distinct cooling zones, with higher intensity near the stagnation points, where the Critical Heat Flux (CHF) recorded of 4.29 MW/m2 corresponds to a heat transfer coefficient of 19.46 KW/(m2 · K). This CHF value decreases by 50% in the jet overlap points and ranges between 39% to 43% of CHF in the upper and lower zones of the channel, perfectly aligning with bibliographic references and thereby enhancing the credibility of the model. This three-dimensional model based on the inverse method, used to calculate thermal parameters, demonstrates remarkable efficiency with an average relative error of 3%, considering the difference in cooled mass between each side of the channel and the heterogeneity of cooling between the inlet and outlet of the channel.
The detailed analysis of local and zonal parameters highlights the importance of the precise position of the jets. This study provides a comprehensive view of the challenges related to cooling cylindrical surfaces. These results enrich the field of thermal management, paving the way for enhanced applications of this technique in critical industrial environments.
Numerical Estimation of Heat Transfer Parameters: A Study on Inverse Methods in Combustion Chambers
Authors: Tahan Meryem , Özkökdemir Emir 
Published: September 2024

Abstract
Inverse heat transfer problems have consistently remained a crucial focal point in research and improvement efforts. These problems typically provide an opportunity to approximate the determination of a property or condition, in cases where it is not possible to measure a boundary condition or a thermophysical property of a material, using various methods based on measured values. The unknown heat transfer can be estimated by using a parameter or function estimation approach with the inverse heat transfer method. If the functional form of the unknown parameters is known, the inverse problem can be solved to determine several parameters within the function. Conversely, if there is no information regarding the functional form of the problem, function estimation can be performed to find the solution. In the thermal design of systems with complex heat flows, such as combustion chambers, it is important to clearly know the thermal loads to which the inner surface of the structure is subjected. It is not possible to obtain these loads by direct measurements. On the other hand, heat transfer parameters at the inner surface can be estimated by inverse methods using measurements taken from the outer surface. With the inverse method mentioned in this study, the heat transfer parameters at the inner surface of a combustion chamber are numerically estimated using temperature measurements taken from the outer surface. A generic combustion chamber is modelled by conduction based finite element method. The unknown heat transfer parameters of the model are iteratively obtained by an optimization algorithm. Subsequently, a variety of optimization methods are utilized to minimize predictive errors, thereby evaluating the uniqueness of the resulting solution.
Examining the Symmetry of a Turbulent Wake Arising From Asymmetric Turbulent Shear Layers: An Experimental and Analytical Study
Authors: Momayez Ladan , Dghim Marouen , Ferchichi Mohsen , Daneshmand Farhang 
Published: September 2024

Abstract
This study examines the wake of a plate subject to asymmetric boundary layers at the trailing edge. In contrast to previous studies, this work varies shear layer asymmetry using tripping wires while keeping the Reynolds number and pressure constant. Six distinct conditions are considered, encompassing the natural wake and asymmetric wakes for five different values of θ/θo where θ is the boundary layer momentum thickness of the disturbed upper plate side, while θo is momentum thickness of the natural boundary on the lower plate side. Both boundary layers are turbulent, and the wake flow statistics were measured at downstream position, x/h. These conditions were studied to gain deeper insight into wake evolution and self-similarity. Significant changes in velocity profiles were observed with increasing momentum thickness ratio. The asymmetric wake showed distinct differences, including a velocity defect (Ud = Ue – Ū) where Ue is edge velocity of the wake and Ū is mean velocity and higher shear stress compared to the symmetric case. The study suggests the presence of long memory in wakes due to self-preserving states established by initial conditions.
Developing analytic methods in conjunction with Reynolds stress profile and Reynolds stress shape functions, we were able to formulate a self-similarity equation for the wake.
Thermoelectric Waste Heat Recovery in a Concentric Tube Silencer for Automotive System
Authors: Lucidi Michael , Farouk Bakhtier 
Published: September 2024

Abstract
In this work, a concentric tube silencer is modeled and studied for its thermodynamic properties using time-dependent compressible computational fluid dynamics. The silencer is simultaneously studied for its capabilities to transfer heat to thermoelectric devices surrounding the silencer, as well as its ability to reduce noise. Two silencer geometries, one with round and the other with square cross-sections, are considered. Efforts have been conducted to recapture the heat from the exhaust for reuse within the automotive system. One such effort involved the ’modified silencing heat exchanger’ recently proposed to replace the muffler of automobiles with a passive device that simultaneously silences the outgoing exhaust while collecting heat. As the muffler is intrinsically involved in treating the loud exhaust produced by an automotive engine, it serves as an ideal location for recapturing the heat generated by said engines and overall improving the efficiency of the system that it is tied into. Previous work has found that the heat transfer to the walls of a concentric tube silencer has a noticeable effect on the frequency bands of the silencer’s overall transmission loss characteristics.
Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in
Acinetobacter pittii
Authors: Van Fossen Elise M. , Kroll Jared O. , Anderson Lindsey N. , McNaughton Andrew D. , Herrera Daisy , Oda Yasuhiro , Wilson Andrew J. , Nelson William C. , Kumar Neeraj , Frank Andrew R. , Elmore Joshua R. , Handakumbura Pubudu , Lin Vivian S. , Egbert Robert G. 
Published: September 2024

ABSTRACT

Interactions between plants and soil microbial communities that benefit plant growth and enhance nutrient acquisition are driven by the selective release of metabolites from plant roots, or root exudation. To investigate these plant-microbe interactions, we developed a photoaffinity probe based on sorgoleone (
so
rgoleone
d
iazirine
a
lkyne for
p
hoto
a
ffinity
l
abeling, SoDA-PAL), a hydrophobic secondary metabolite and allelochemical produced in
Sorghum bicolor
root exudates. We applied SoDA-PAL to the identification of sorgoleone-binding proteins in
Acinetobacter pittii
SO1, a potential plant growth-promoting microbe isolated from sorghum rhizosphere soil. Competitive photoaffinity labeling of
A. pittii
whole cell lysates with SoDA-PAL identified 137 statistically enriched proteins, including putative transporters, transcriptional regulators, and a subset of proteins with predicted enzymatic functions. We performed computational protein modeling and docking with sorgoleone to prioritize candidates for experimental validation and then confirmed binding of sorgoleone to four of these proteins
in vitro
: the α/β fold hydrolase SrgB (OH685_09420), a fumarylacetoacetase (OH685_02300), a lysophospholipase (OH685_14215), and an unannotated hypothetical protein (OH685_18625). Our application of this specialized sorgoleone-based probe coupled with structural bioinformatics streamlines the identification of microbial proteins involved in metabolite recognition, metabolism, and toxicity, widening our understanding of the range of cellular pathways that can be affected by a plant secondary metabolite.

IMPORTANCE

Here, we demonstrate that a photoaffinity-based chemical probe modeled after sorgoleone, an important secondary metabolite released by sorghum roots, can be used to identify microbial proteins that directly interact with sorgoleone. We applied this probe to the sorghum-associated bacterium
Acinetobacter pittii
and showed that probe labeling is dose-dependent and sensitive to competition with purified sorgoleone. Coupling the probe with proteomics and computational analysis facilitated the identification of putative sorgoleone binders, including a protein implicated in a conserved pathway essential for sorgoleone catabolism. We anticipate that discoveries seeded by this workflow will expand our understanding of the molecular mechanisms by which specific metabolites in root exudates shape the sorghum rhizosphere microbiome.
Identification of a chlorosalicylic acid decarboxylase (CsaD) involved in decarboxylation of 3,6-DCSA from an anaerobic dicamba-degrading sludge
Authors: Zhang Xuan , Wu Ningning , Geng Keke , Yuan Cansheng , Wang Baozhan , Shi Junyu , Qiu Jiguo , He Jian 
Published: September 2024

ABSTRACT

3,6-Dichlorosalicylic acid (3,6-DCSA) is the demethylation metabolite of herbicide 3,6-dichloro-2-methoxy benzoic acid (dicamba). Previous studies have shown that anaerobic sludge further transformed 3,6-DCSA through decarboxylation and dechlorination. However, the anaerobe, enzyme, and gene involved in the anaerobic degradation of 3,6-DCSA are still unknown. In this study, an anaerobic sludge that efficiently degraded dicamba was enriched, and a 3,6-DCSA decarboxylase, designated chlorosalicylic acid decarboxylase (CsaD), was partially purified and identified from the anaerobic sludge. Metagenomic analysis showed that the
csaD
gene was located in a gene cluster of metagenome-assembled genome 8 (MAG8). MAG8 belonged to an uncultured order, OPB41, in the class
Coriobacteriia
of the phylum
Actinobacteria
, and its abundance increased approximately once during the enrichment process. CsaD was a non-oxidative decarboxylase in the amidohydrolase 2 family catalyzing the decarboxylation of 3,6-DCSA and 6-chlorosalicylic acid (6-CSA). Its affinity and catalytic efficiency for 3,6-DCSA were significantly higher than those for 6-CSA. This study provides new insights into the anaerobic catabolism of herbicide dicamba.

IMPORTANCE
Dicamba, an important hormone herbicide, easily migrates to anoxic habitats such as sediment, ground water, and deep soil. Thus, the anaerobic catabolism of dicamba is of importance. Anaerobic bacteria or sludge demethylated dicamba to 3,6-DCSA, and in a previous study, based on metabolite identification, it was proposed that 3,6-DCSA be further degraded via two pathways: decarboxylation to 2,5-dichlorophenol, then dechlorination to 3-chlorophenol (3-CP); or dechlorination to 6-CSA, then decarboxylation to 3-CP. However, there was no physiological and genetic validation for the pathway. In this study, CsaD catalyzed the decarboxylation of both 3,6-DCSA and 6-CSA, providing enzyme-level evidence for the anaerobic catabolism of 3,6-DCSA through the two pathways. CsaD was located in MAG8, which belonged to an uncultured anaerobic actinomycetes order, OPB41, indicating that anaerobic actinomycetes in OPB41 was involved in the decarboxylation of 3,6-DCSA. This study provides a basis for understanding the anaerobic catabolism of dicamba and the demethylation product, 3,6-DCSA.