bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration
Authors: Zhang Meng , Wang Hai‐Yan , Zhang Yu‐Qing 
Published: September 2024

AbstractThe immobilization of free enzymes is crucial for enhancing their stability in different environments, enabling reusability, and expanding their applications. However, the development of a straightforward immobilization method that offers stability, high efficiency, biocompatibility, and modifiability remains a significant challenge. Silk fibroin (SF) is a good carrier for immobilized enzymes and drugs. Here, we employed urease as a model enzyme and utilized our developed technology called unidirectional nanopore dehydration (UND) to efficiently dehydrate a regenerated SF solution containing urease in a single step, resulting in the preparation of a highly functionalized SF membrane immobilizing urease (UI‐SFM). The preparation process of UI‐SFM is based on an all‐water system, which is mild, green and able to efficiently and stably immobilize urease in the membranes, maintaining 92.7% and 82.8% relative enzyme activity after 30 days of storage in dry and hydrated states, respectively. Additionally, we performed additional post‐treatments, including stretching and cross‐linking with polyethylene glycol diglycidyl ether (PEGDE), to obtain two more robust immobilized urease membranes (UI‐SFMs and UI‐SFMc). The thermal and storage stability of these two membranes were significantly improved, and the recovery ratio of enzyme activity reached more than 90%. After 10 repetitions of the enzymatic reaction, the activity recovery of UI‐SFMs and UI‐SFMc remained at 92% and 88%, respectively. The results suggest that both UND‐based and post‐treatment‐developed membranes exhibit excellent urease immobilization capabilities. Furthermore, the enzyme immobilization method offers a straightforward and versatile approach for efficient and stable enzyme immobilization, while its flexible modifiability caters to diverse application requirements.
Preclinical evaluation of avutometinib and defactinib in high‐grade endometrioid endometrial cancer
Authors: Hartwich Tobias Max Philipp , Mansolf Miranda , Demirkiran Cem , Greenman Michelle , Bellone Stefania , McNamara Blair , Nandi Shuvro P. , Alexandrov Ludmil B. , Yang‐Hartwich Yang , Coma Silvia , Pachter Jonathan , Santin Alessandro D. 
Published: September 2024

AbstractBackgroundHigh‐grade endometrial cancers (EAC) are aggressive tumors with a high risk of progression after treatment. As EAC may harbor mutations in the RAS/MAPK pathways, we evaluated the preclinical in vitro and in vivo efficacy of avutometinib, a RAF/MEK clamp, in combination with the focal adhesion kinase (FAK) inhibitors defactinib or VS‐4718, against multiple primary EAC cell lines and xenografts.MethodsWhole‐exome sequencing (WES) was used to evaluate the genetic landscape of five primary EAC cell lines. The in vitro activity of avutometinib and defactinib as single agents and in combination was evaluated using cell viability, cell cycle, and cytotoxicity assays. Mechanistic studies were performed using Western blot assays while in vivo experiments were completed in UTE10 engrafted mice treated with either vehicle, avutometinib, VS‐4718, or their combination through oral gavage.ResultsWES results demonstrated multiple EAC cell lines to harbor genetic derangements in the RAS/MAPK pathway including KRAS/PTEN/PIK3CA/BRAF/ARID1A, potentially sensitizing to FAK and RAF/MEK inhibition. Five out of five of the EAC cell lines demonstrated in vitro sensitivity to FAK and/or RAF/MEK inhibition. By Western blot assays, exposure of EAC cell lines to defactinib, avutometinib, and their combination demonstrated decreased phosphorylated FAK (p‐FAK) as well as decreased p‐MEK and p‐ERK. In vivo the combination of avutometinib/VS‐4718 demonstrated superior tumor growth inhibition compared to single‐agent treatment and controls starting at Day 9 (p < 0.02 and p < 0.04) in UTE10 xenografts.ConclusionsAvutometinib, defactinib, and to a larger extent their combinations, demonstrated promising in vitro and in vivo activity against EAC cell lines and xenografts. These preclinical data support the potential clinical evaluation of this combination in high‐grade EAC patients.
Financial well‐being: An integrated framework, operationalization, and future research agenda
Authors: Garg Nishant , Priyadarshi Pushpendra , Malik Ashish 
Published: September 2024

AbstractFinancial well‐being occupies a pivotal juncture between money and happiness, serving as fundamental components for favorable individual and organizational outcomes. The exponential growth in literature seeking to delineate its conceptualization, mechanisms, and enablers underscores its significance and implications. However, the varied scholarly contributions have also given rise to persistent discrepancies and a subjective‐objective dichotomy within the field, necessitating a comprehensive review. To address this gap, we conducted a thorough bibliometric analysis employing citation, co‐citation, and co‐occurrence techniques. Our study enriches the theoretical underpinnings by examining its thematic nuances, underlying nomological networks, and evolutionary trends. Specifically, our findings unveil a notable shift toward subjective evaluation shaped by temporal perspectives. Moreover, we discern an increasing influence of psychological variables in shaping individuals' subjective assessments of their financial well‐being, extending beyond traditional cognitive and objective dimensions. Additionally, our analysis underscores the pivotal role of financial socialization, highlighting the impact of social and environmental factors on subjective perceptions. These insights are encapsulated within our proposed operationalization, integrated framework, and future research direction.
Oxidase‐Like Nanozyme Activity of Ultrathin Copper Metal–Organic Framework Nanosheets With High Specificity for Catechol Oxidation
Authors: Singh Ajit Kumar , Sharma Deepika , Singh Devesh Kumar , Sarraf Sonu , Basu Aviru Kumar , Ganesan Vellaichamy , Saha Avishek , Indra Arindam 
Published: September 2024

AbstractIn nature, catechol oxidase catalyzes the oxidation of o‐diphenol to o‐quinone to produce a series of highly important polyphenolic natural products. Although mimicking the functionality of natural enzyme using a nanozyme was found to be beneficial, attaining a high specificity is challenging. Herein, we have explored the thickness‐dependent oxidase activity and specificity of Cu‐metal‐organic framework (MOF) nanosheets. The unique synthetic method offers control over the thickness of the Cu‐MOF nanosheets. The ultrathin (4 nm) Cu‐MOF (Cu‐UMOF) nanosheets as an oxidase nanozyme exhibit high specificity for catechol oxidation without having any peroxidase activity. Interestingly, the thicker (20–30 nm) Cu‐MOF nanosheets showed poor catechol oxidase and peroxidase activity. The binuclear Cu‐center, coordinative, and electronic unsaturation tuned electronic structure in Cu‐UMOF, resulting in higher specificity for catechol oxidation than thicker Cu‐MOF.
Thienyl‐Bridged Fused Porphyrin Tapes for Enhanced Heterogeneous Electrocatalytic Activity
Authors: Ghahramanzadehasl Hadi , Bansal Deepak , Cardenas Morcoso Drialys , Boscher Nicolas D. 
Published: September 2024

AbstractThe development of highly conjugated metalloporphyrin assemblies is a crucial step to improve their catalytic activity for optimal energy conversion processes. Herein, di‐thienyl substituted nickel(II) porphyrin is used to form a highly conjugated porphyrin structure. The resulting porphyrin‐based conjugated polymer catalyst exhibited exceptional oxygen evolution reaction “OER” performances, featuring a low onset overpotential of 266 mV and high reaction kinetics (Tafel slope of 69.9 mV/dec) under alkaline pH conditions, achieving a current density of 4.5 mA/cm2. The remarkable OER catalytic activity of porphyrin‐based conjugated polymer catalyst is attributed to the enhancement of the conjugation, which occurs through a unique process involving direct fusion of the porphyrins followed by thienyl bridging of the fused porphyrin tapes, ultimately leading to the establishment of a highly cross‐linked porphyrinic network.
Impedance Spectroscopy of Lithium Intercalation into Cathode Materials in Coin Cells
Authors: Yesilbas Göktug , Grieve Daniel , Rettmann David , Gülderen Kivanc , Bandarenka Aliaksandr S. , Yun Jeongsik 
Published: September 2024

AbstractUnderstanding the internal reactions in Li‐ion batteries is crucial to analyze them more accurately and improve their efficiency since they are involved in almost every aspect of everyday life. Electrochemical impedance spectroscopy is a valuable research technique to investigate such batteries, as it reveals sensitive properties and essential information about cell reaction mechanisms and kinetics. Physical understanding of the electrochemical process and system of a battery can be analyzed using equivalent electric circuits (EECs) with rational selection of electric circuit elements and their combination. However, impedance analysis of a battery is often conducted using oversimplified EEC models in practice due to the complexity and difficulty of the physics and mathematics of the modeling. This study proposes and verifies an EEC model that represents a three‐stage mechanism for intercalation‐type materials. For the systematic model study and verifications, we investigated cathode half cells using four different layered structured cathode materials, namely, LiCoO2, LiNi1/3Mn1/3Co1/3O2, LiNi0.9Mn0.05Co0.05O2, and Ni0.815Co0.15Al0.035O2. Parametric analysis of the impedance fittings for the four different cathode materials showed similar behavior depending on the states of charge. We also provided the complete set of parameters of the four systems: charge transfer resistance, double‐layer capacitance, and solid‐electrolyte interphase (SEI) resistance and capacitance. Lastly, we explain how different electrochemical processes, such as intercalation and alloying, can be analyzed and modeled in EEC models.
Metal chloride‐graphite intercalation compounds for rechargeable metal‐ion batteries
Authors: Lu Anbang , Wang Fei , Liu Zhendong , Wang Yuchen , Gu Yue , Wang Shuang , Ye Chong , Liu Quanbing , Zhang Chengzhi , Tan Jun 
Published: September 2024

AbstractThe typical metal chloride‐graphite intercalation compounds (MC‐GICs) inherit intercalation capacity, high charge conductivity, and high tap density from graphite, and these are considered as one of the promising alternatives of graphite anode in rechargeable metal‐ion batteries (MIBs). Notably, the special interlayer decoupling effects and the introduction of extra conversion capacity by metal chloride could greatly break the capacity limitation of graphite anodes and achieve higher energy density in MIBs. The optimization of both graphite host and metal chloride species with specific structures endows MC‐GICs with design feasibility for different application requirements of different MIBs, such as several times the actual capacity compared to graphite anodes, rapid migration of large carriers, and other properties. Herein, a brief review has been provided with the latest understanding of conductivity characteristics and energy storage mechanisms of MC‐GICs and their interesting performance features of full potential application in rechargeable MIBs. Based on the existing research of MC‐GICs, necessary improvements and prospects in the near future have been put forward.
On the Valorisation of Chitin‐Derived Furans by Milling
Authors: Mattioli Renan Rodini , Santos Camila Souza , de Souza Bruna Butke , Branco Pedro Dominguez , Bolt Robert R. A. , Raby‐Buck Sarah E. , Gomes Cabral Tadeu Luiz , Tormena Claudio F. , Browne Duncan L. , Pastre Julio C. 
Published: September 2024

AbstractChitin‐derived furans offer a sustainable alternative feedstock for nitrogen appended aromatic compounds. Herein, we address the challenge of using chitin‐derived furans, 3‐acetamido‐5‐acetylfuran (3A5AF) and 3‐acetamido‐5‐furfural aldehyde (3A5F), to favour the formation of exo Diels–Alder adducts and 4‐acetylaminophthalimides respectively, using a mechanochemical ball‐milling technique. Mechanochemical activation is explored through the synthesis of 7‐oxa‐norbornene backbones with novel substitution pattern from 3A5AF in yields up to 77 % and improved exo:endo selectivity compared to solution‐phase reactions. The synthesis of 4‐acetylaminophthalimides from 3A5F in yields up to 79 % is also showcased from hydrazone derivatives.
Porous Organic Polymers as Ionomers for High‐Performance Alkaline Membrane Water Electrolysis
Authors: Rico‐Martínez Sandra , Cho Hyeon Keun , Hu Chuan , Lee Young Jun , Miguel Jesús A. , Lozano Angel E. , Lee Young Moo 
Published: September 2024

AbstractSustainable hydrogen production is focused on anion exchange membrane (AEM) water electrolyzers (AEMWEs), which still require more development to achieve high performance and durability. Here, we propose a novel class of porous organic polymers (POPs) as durable solid‐ionomers for AEMWEs, which was prepared by reacting the 4‐methylpiperidone with trifunctional or a mixture of trifunctional:difunctional aromatic monomers (in a 2 : 3 mol ratio). The resulting POP ionomers exhibited exceptional electrochemical properties and remarkable alkaline stability. Particularly noteworthy are the corresponding AEMWEs, which showed an outstanding current density of 13.4 A cm−2 at 2.0 V under 80 °C in 1 M KOH solution, which is the highest performance reported in the particulate‐ionomers AEMWE state of the art. Moreover, they demonstrated durability at a current density of 0.5 A cm−2 for over 500 h with a voltage decay rate of 120 μV h−1. This work offers valuable perspectives on the designing of robust and high‐performance solid‐state ionomers through low‐cost electrophilic aromatic substitution reactions for high‐performance energy conversion devices.
Novel Graphitic Oxynitrides as Photocatalysts for Sustainable H2 Production and CO2 Valorization. The Importance of Self‐Assembly for Catalytic Activity
Authors: Lavado Nieves , Pardo‐Botello Rosario , María Sánchez‐Rodas José , Fernando Martínez R. , Montes Vicente , Javier López‐Tenllado Francisco , Cintas Pedro , Babiano Reyes 
Published: September 2024

AbstractThe field of carbocatalysis, often portrayed by paradigmatic graphitic carbonaceous structures, has become a booming topic tailored for multiple applications. To this end, a new metal‐free carbocatalyst has been constructed from simple prebiotic monomers such as cyanamide and glyoxal. The resulting material shows an excellent performance as photocatalyst for H2 production and CO2 valorization, thus unveiling its real value to tackle sustainable goals. The unique oxygen‐rich carbonaceous structure has been characterized in detail, which is consistent with a graphitic layered network. The described performance in two major societal concerns along with a facile preparation from C1/C2 platforms, makes this type of overlooked oxynitride carbocatalysts promising for real‐life environmental endeavors.
Idiosyncratic Reaction of Isotretinoin: A Review
Authors: Lee Kar Wai Alvin , Chan Lisa Kwin Wah , Lee Cheuk Hung , Wan Jovian , Yi Kyu‐Ho 
Published: September 2024

ABSTRACTBackgroundIsotretinoin is widely recognized for its efficacy in treating severe acne. However, its use is associated with a variety of idiosyncratic reactions that can affect multiple organ systems and bodily functions, necessitating vigilant monitoring and appropriate management.ObjectivesThe aim of this review is to raise awareness of the broad spectrum of adverse effects linked to isotretinoin therapy, thereby enhancing the ability of healthcare providers to identify at‐risk individuals and improve patient outcomes.MethodsA comprehensive review of current literature was conducted to explore the range of idiosyncratic reactions associated with isotretinoin. The focus was on identifying the types of adverse effects reported and the necessary monitoring strategies to mitigate these risks.ResultsIsotretinoin has been linked to a wide array of adverse effects, including, but not limited to, psychiatric issues (e.g., depression and suicidal behavior), hematological complications (e.g., neutropenia and agranulocytosis), gastrointestinal disturbances, cardiovascular effects, dermatological manifestations, hepatobiliary issues, ophthalmological changes, and orthopedic concerns. These reactions highlight the importance of careful patient monitoring during isotretinoin therapy.ConclusionHealthcare professionals prescribing isotretinoin must be aware of the potential for diverse idiosyncratic reactions and should implement appropriate monitoring protocols to ensure patient safety. By understanding and anticipating these adverse effects, healthcare providers can better manage the risks associated with isotretinoin use, ultimately improving the quality of care for patients undergoing treatment for severe acne.
The genus Eutreptiella (Euglenophyceae/Euglenozoa) across its global distribution range
Authors: Hernández Márquez Sinuhé , Zamudio‐Resendiz María Eugenia , Murrieta‐Alarcón Montserrat , Núñez Resendiz María Luisa , Dreckmann Kurt M. , Sentíes Abel 
Published: September 2024

AbstractThe genus Eutreptiella (Euglenophyceae/Euglenozoa) comprises unicellular organisms known for their photosynthetic capacity and significant role in marine ecosystems. This review highlights the taxonomic, ecological, and biotechnological characteristics of Eutreptiella species, emphasizing their morphological and genomic adaptations. Eutreptiella species exhibit high phenotypic plasticity, enabling adaptation to various environmental conditions, from nutrient‐rich waters to high‐salinity conditions. They play a crucial role in primary production and nutrient cycling in marine ecosystems. Genetic and transcriptomic studies have revealed their complex regulatory mechanisms and potential for biofuel and nutraceutical production. Eutreptiella blooms significantly impact local ecosystems, influencing nutrient availability and community dynamics. Additionally, interactions with associated bacteria enhance their growth and metabolic capabilities. The genus shows substantial genetic variability, suggesting potential misidentifications or a polyphyletic nature. Further comprehensive studies are needed to clarify their taxonomy and evolutionary relationships. Understanding and managing Eutreptiella populations is essential to leverage their biotechnological potential and ensure the health of marine ecosystems.
Experimental assessment of environmental versus genetic influences on Macrocystis morphology
Authors: Gonzalez Sara T. , Raimondi Peter T. 
Published: September 2024

AbstractThe two most common ecomorphs of giant kelp, Macrocystis pyrifera and Macrocystis integrifolia, exhibit almost no geographic overlap across their range in the temperate waters of North and South America, with few exceptions such as Stillwater Cove, CA, USA. However, in laboratory settings they are able to interbreed. The mechanism for the morphological variation is unknown, and whether these features are genetically fixed or malleable under different environmental conditions is unclear. Our study, for the first time, directly compared the growth, development, and morphological transformations of the two Macrocystis ecomorphs in a common garden experiment. We experimentally tested the influence of local environment on giant kelp morphology by rearing lab‐cultured embryonic sporophytes from spores released by M. pyrifera and M. integrifolia sporophylls collected from multiple individuals at Stillwater Cove, CA. The spores were cultured in three treatments: “pyrifera‐morph” only, “integrifolia‐morph” only, and mixed (50:50 “pyrifera‐morph”:“integrifolia‐morph” spores). We outplanted the resultant embryonic sporophytes to concrete blocks installed at 7.5–9 m depth in the ocean and monitored the development of the sporophytes over 5 months. Our findings of distinct differences in morphology between the pyrifera‐treatment and integrifolia‐treatment individuals at multiple stages of development including reproductive adults indicates that the morphological differences between the two ecomorphs are genetically determined rather than environmentally induced. We found that primary stipe length and number of branches can be used as diagnostic traits for distinguishing the ecomorphs prior to the stage when adult sporophyte morphology can be definitively characterized. Additionally, no morphological hybrids were observed in the mixed‐treatment, and ultimately the mixed‐treatment individuals were more often categorized as “integrifolia‐morph”‐like than “pyrifera‐morph”‐like.
An Electrochemical Bioplatform Based on Acrylamide‐Induced Inhibition of DNA‐RNA Hybridization for the Determination of Acrylamide
Authors: Zouari Mohamed , Ruiz‐Valdepeñas Montiel Víctor , Gamella María , Setti Boubaker Nouha , Pingarrón José M. , Campuzano Susana , Raouafi Noureddine 
Published: September 2024

AbstractThis study reports the development of an innovative electrochemical bioplatform for the indirect determination of acrylamide (AM) based on its inhibitory effect on the hybridization of nucleic acids through the formation of AM‐guanine single‐stranded (ssDNA) adducts. The method combines the advantages of the formation and selective immunorecognition of DNA/RNA heteroduplex, with the use of MBs and amperometric transduction using disposable SPCEs. Increasing concentrations of acrylamide prevented the efficient hybridization of a synthetic biotinylated DNA probe immobilized on streptavidin‐coated magnetic beads with its complementary RNA target and the as‐formed heteroduplex recognition with a specific antibody and a secondary HRP‐labeled antibody used for electrochemical readout. The resulting magnetic bioconjugates were magnetically trapped on the surface of the working electrode of a SPCE and the amperometric transduction was carried out in the presence of the H2O2/HQ system, recording a cathodic response inversely proportional to the AM concentration. The developed bioplatform is more competitive with other reported methods in terms of simplicity and assay time, allowing the selective detection of 1 nM AM in 60 min. In addition, the bioplatform was applied to the analysis of potato crisp water extracts.
Performance estimation of a two‐body wave energy converter for the East Coast of India
Authors: Singh Devesh , Paul Akshoy Ranjan , Samad Abdus 
Published: September 2024

AbstractThe study aims to assess the feasibility of harnessing wave energy from the low‐energy waves prevalent along the east coast of India, where wave energy converters (WECs) may not produce sufficient power economically. Vishakhapatnam port, located on the east coast, experiences such waves (period, Tp = 5 to 10 s, and wave height, Hs = 0.07 to 2.61 m). This study analyzes the performance of a two‐body point absorber WEC and estimates the wave power using the parameters Hs and Tp. The hydrodynamic parameters were assessed in the frequency domain using the boundary element method, while the output power was estimated in the time domain using the open‐source code WEC‐Sim. The harvested power initially increased with the wave period, peaked at Tp = 7 s and Hs = 2 m, and subsequently decreased. This study highlights the potential of wave energy as a renewable resource for India, given its extensive coastline and untapped wave energy potential. It suggests that green energy firms should explore opportunities in regions abundant with wave energy to promote sustainable energy production and drive innovation in wave energy technology, thereby indicating avenues for future research in this domain.