bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Empirical Comparison Between the Synthetic Control Method and HSIAO et al.'s Panel Data Approach to Program Evaluation
Authors: Gardeazabal Javier , Vega‐Bayo Ainhoa 
Published: December 2016

SummaryWe compare two program evaluation methodologies: the synthetic control method and the panel data approach. We apply both methods to estimate the effect of the political and economic integration of Hong Kong. The results obtained differ depending on the methodology used. We then conduct a simulation that shows that the synthetic control method results in a post‐treatment mean squared error, mean absolute percentage error, and mean error with a smaller interquartile range, whenever there is a good enough match. Copyright © 2016 John Wiley & Sons, Ltd.
FGF Suppresses Poldip2 Expression in Osteoblasts
Authors: Katsumura Sakie , Izu Yayoi , Yamada Takayuki , Griendling Kathy , Harada Kiyoshi , Noda Masaki , Ezura Yoichi 
Published: December 2016

ABSTRACTOsteoporosis is one of the most prevalent ageing‐associated diseases that are soaring in the modern world. Although various aspects of the disease have been investigated to understand the bases of osteoporosis, the pathophysiological mechanisms underlying bone loss is still incompletely understood. Poldip2 is a molecule that has been shown to be involved in cell migration of vascular cells and angiogenesis. However, expression of Poldip2 and its regulation in bone cells were not known. Therefore, we examined the Poldip2 mRNA expression and the effects of bone regulators on the Poldip2 expression in osteoblasts. We found that Poldip2 mRNA is expressed in osteoblastic MC3T3‐E1 cells. As FGF controls osteoblasts and angiogenesis, FGF regulation was investigated in these cells. FGF suppressed the expression of Poldip2 in MC3T3‐E1 cells in a time dependent manner. Protein synthesis inhibitor but not transcription inhibitor reduced the FGF effects on Poldip2 gene expression in MC3T3‐E1 cells. As for bone‐related hormones, dexamethasone was found to enhance the expression of Poldip2 in osteoblastic MC3T3‐E1 cells whereas FGF still suppressed such dexamethasone effects. With respect to function, knockdown of Poldip2 by siRNA suppressed the migration of MC3T3‐E1 cells. Poldip2 was also expressed in the primary cultures of osteoblast‐enriched cells and FGF also suppressed its expression. Finally, Poldip2 was expressed in femoral bone in vivo and its levels were increased in aged mice compared to young adult mice. These data indicate that Poldip2 is expressed in osteoblastic cells and is one of the targets of FGF. J. Cell. Biochem. 118: 1670–1677, 2017. © 2016 Wiley Periodicals, Inc.
Type 2 Diabetes Inhibited Human Mesenchymal Stem Cells Angiogenic Response by Over‐Activity of the Autophagic Pathway
Authors: Rezabakhsh Aysa , Cheraghi Omid , Nourazarian Alireza , Hassanpour Mehdi , Kazemi Masoumeh , Ghaderi Shahrooz , Faraji Esmaeil , Rahbarghazi Reza , Avci Çığır Biray , Bagca Bakiye Goker , Garjani Alireza 
Published: December 2016

ABSTRACTThe current study aimed to address the impact of serum from type 2 diabetes patients on the angiogenic properties of human bone marrow mesenchymal stem cells and its relationship to autophagy signaling. Human primary stem cells were enriched and incubated with serum from diabetic and normal subjects for 7 days. Compared to data from the control group, diabetic serum was found to induce a higher cellular death rate (P < 0.001) and apoptotic changes (P < 0.01). We also showed that diabetic condition significantly abolished angiogenesis tube formation on Matrigel substrate, decreased cell chemotaxis (P < 0.01) in response to SDF‐1α, and inhibited endothelial differentiation rate (P < 0.0001). Western blotting showed autophagic status by high levels of P62 (P < 0.0001), beclin‐1 (P < 0.0001), and increase in LC3II/I ratio (P < 0.001). In vivo Matrigel plug assay revealed that supernatant conditioned media prepared from cells exposed to diabetic serum caused a marked reduction in the recruitment of VE‐cadherin‐ (P < 0.01) and α‐SMA‐positive (P < 0.0001) cells 7 days after subcutaneous injection. PCR expression array analysis confirmed the overexpression of autophagy and apoptosis genes in cultured cells in response to a diabetic condition (P < 0.05). Using bioinformatic analysis, we noted a crosstalk network between DM2, angiogenesis, and autophagy signaling. DM2 could potently modulate angiogenesis by the interaction of IL‐1β with downstream insulin receptor and upstream androgen receptor. Corroborating to data, diabetic serum led to abnormal regulation of P62 during the angiogenic response. These data demonstrate that diabetic serum decreased human mesenchymal stem cell angiogenic properties directly on angiogenesis pathways or by the induction of autophagy signaling. J. Cell. Biochem. 118: 1518–1530, 2017. © 2016 Wiley Periodicals, Inc.
Resveratrol Inhibits Proliferation, Invasion, and Epithelial–Mesenchymal Transition by Increasing miR‐200c Expression in HCT‐116 Colorectal Cancer Cells
Authors: Karimi Dermani Fatemeh , Saidijam Massoud , Amini Razieh , Mahdavinezhad Ali , Heydari Korosh , Najafi Rezvan 
Published: December 2016

ABSTRACTObjective: colorectal cancer (CRC) is one of the most common malignancies, associated with high rates of relapse. A notable challenge in treatment is low response rate to current therapies for advanced CRC. The miR‐200c plays an essential role in tumor suppression by inhibiting epithelial–mesenchymal transition (EMT). Resveratrol, a natural compound found in red wine, reveals anti‐cancer properties in several types of cancers such as CRC. The aim of current study was to evaluate the effects of resveratrol on proliferation, apoptosis, and invasion of HCT‐116 cells and also expression of EMT‐related genes in presences or absence of miR‐200c. Methods: the effect of resveratrol on viability was examined by MTT assay. LNA‐anti‐miR‐200c transfection of HCT‐116 cells was carried out in a time dependent manner. Then, the expression of miR‐200c and EMT‐related genes were quantified by qRT‐PCR. Further, expression of EMT‐related proteins, apoptosis, and invasion were analyzed by Western blot, Annexin V/PI staining and scratch test, respectively. Results: resveratrol could significantly inhibit viability of HCT‐116 cells. LNA‐anti‐miR‐200c suppressed the endogenous miR‐200c in transfected cells compared with the control. qRT‐PCR and Western blot analysis of LNA‐anti‐miR‐200c transfected cells revealed a considerable increase in vimentin and ZEB‐1 expression, with a concomitant reduction in E‐cadherin expression level. Migration of HCT‐116 cells increased, and apoptosis significantly reduced in transfected cells. While, resveratrol could entirely reverse these changes by modulation of miR‐200c expression. Conclusion: our findings revealed a major role of resveratrol in apoptosis, invasion, and switching of EMT to MET phenotype through upregulation of miR‐200c in CRC. J. Cell. Biochem. 118: 1547–1555, 2017. © 2016 Wiley Periodicals, Inc.
Landscape attributes explain migratory caribou vulnerability to sport hunting
Authors: Plante Sabrina , Dussault Christian , Côté Steeve D. 
Published: December 2016

ABSTRACTHuman disturbances are increasing in Arctic regions and have been suggested as one of the main factors explaining caribou (Rangifer tarandus) decline. The cumulative effects of disturbances may negatively affect caribou habitat use, survival, and population dynamics. Thus, there is a need to evaluate the impact of various human disturbances, especially those that cause direct mortality (e.g., sport hunting). We evaluated the relative importance of caribou and hunter habitat selection and landscape characteristics on caribou vulnerability to sport hunting in northern Québec, Canada. We used resource selection functions to describe habitat selection of 223 caribou and 87 hunters. We then characterized >169,000 caribou harvest sites recorded over 17 years according to the relative probability of co‐occurrence of caribou and hunters, the relative probability of occurrence of hunters only, or the characteristics of the landscape (e.g., distance to human infrastructures, elevation, land cover type). Landscape characteristics better explained caribou vulnerability to sport hunting than habitat selection of caribou and hunters, or their co‐occurrence. Caribou were more vulnerable in proximity to hunting infrastructures (e.g., roads, outfitter camps) than elsewhere, but caribou strongly avoided roads. Caribou were also more vulnerable on frozen lakes than in other land cover types. Lakes were, however, avoided by caribou and not selected by hunters. Harvest was more likely in smoother terrain, even if caribou and hunters did not select for this characteristic. We demonstrated caribou were more vulnerable in areas with good accessibility (near roads) or where caribou were easily detectable (lakes, smoother terrain), which also represents areas that were either avoided or not selected by caribou or hunters. This discrepancy between harvest distribution and behaviors of caribou and hunters suggests that harvest may be an opportunistic event where visibility and accessibility increased chances of success for hunters. Managers could use this information to manipulate hunting success according to population estimates and harvest quota by establishing minimal distance to risky areas within which hunting would be prohibited. © 2016 The Wildlife Society.
Evaluation of the Biocompatibility of Polypyrrole Implanted Subdurally in GAERS
Authors: Bauquier Sebastien H. , McLean Karen J. , Jiang Jonathan L. , Boston Ray C. , Lai Alan , Yue Zhilian , Moulton Simon E. , Halliday Amy J. , Wallace Gordon , Cook Mark J. 
Published: December 2016

This blinded controlled prospective randomized study investigates the biocompatibility of polypyrrole (PPy) polymer that will be used for intracranial triggered release of anti‐epileptic drugs (AEDs). Three by three millimeters PPy are implanted subdurally in six adult female genetic absence epilepsy rats from Strasbourg. Each rat has a polymer implanted on one side of the cortex and a sham craniotomy performed on the other side. After a period of seven weeks, rats are euthanized and parallel series of coronal sections are cut throughout the implant site. Four series of 15 sections are histological (hematoxylin and eosin) and immunohistochemically (neuron‐specific nuclear protein, glial fibrillary acidic protein, and anti‐CD68 antibody) stained and evaluated by three investigators. The results show that implanted PPy mats do not induce obvious inflammation, trauma, gliosis, and neuronal toxicity. Therefore the authors conclude the PPy used offer good histocompatibility with central nervous system cells and that PPy sheets can be used as intracranial, AED delivery implant.
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Self‐Assembled Supramolecular Nanogels as a Safe and Effective Drug Delivery Vector for Cancer Therapy
Authors: Cheng Chih‐Chia , Liang Mei‐Chih , Liao Zhi‐Sheng , Huang Jyun‐Jie , Lee Duu‐Jong 
Published: December 2016

Simple construction and manipulation of low‐molecular‐weight supramolecular nanogels, based on the introduction of multiple hydrogen bonding interactions, with the desired physical properties to achieve effective and safe delivery of drugs for cancer therapy remain highly challenging. Herein, a novel supramolecular oligomer cytosine (Cy)‐polypropylene glycol containing self‐complementary multiple hydrogen‐bonded Cy moieties is developed, which undergoes spontaneous self‐assembly to form nanosized particles in an aqueous environment. Phase transitions and scattering studies confirm that the supramolecular nanogels can be readily tailored to obtain the desired phase‐transition temperature and temperature‐induced release of the anticancer drug doxorubicin (DOX). The resulting nanogels exhibit an extremely high load carrying capacity (up to 24.8%) and drug‐entrapment stability, making the loading processes highly efficient. Importantly, in vitro cytotoxicity assays indicate that DOX‐loaded nanogels possess excellent biosafety for drug delivery applications under physiological conditions. When the environmental temperature is increased to 40 °C, DOX‐loaded nanogels trigger rapid DOX release and exert cytotoxic effects, significantly reducing the dose required compared to free DOX. Given its simplicity, low cost, high reliability, and efficiency, this newly developed temperature‐responsive nanocarrier has highly promising potential for controlled release drug delivery systems.
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4D Printing of Shape Memory‐Based Personalized Endoluminal Medical Devices
Authors: Zarek Matt , Mansour Nicola , Shapira Shir , Cohn Daniel 
Published: December 2016

The convergence of additive manufacturing and shape‐morphing materials is promising for the advancement of personalized medical devices. The capability to transform 3D objects from one shape to another, right off the print bed, is known as 4D printing. Shape memory thermosets can be tailored to have a range of thermomechanical properties favorable to medical devices, but processing them is a challenge because they are insoluble and do not flow at any temperature. This study presents here a strategy to capitalize on a series of medical imaging modalities to construct a printable shape memory endoluminal device, exemplified by a tracheal stent. A methacrylated polycaprolactone precursor with a molecular weight of 10 000 g mol−1 is printed with a UV‐LED stereolithography printer based on anatomical data. This approach converges with the zeitgeist of personalized medicine and it is anticipated that it will broadly expand the application of shape memory‐exhibiting biomedical devices to myriad clinical indications.
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Simultaneous orthogonal plane imaging
Authors: Mickevicius Nikolai J. , Paulson Eric S. 
Published: December 2016

PurposeIntrafraction motion can result in a smearing of planned external beam radiation therapy dose distributions, resulting in an uncertainty in dose actually deposited in tissue. The purpose of this paper is to present a pulse sequence that is capable of imaging a moving target at a high frame rate in two orthogonal planes simultaneously for MR‐guided radiotherapy.TheoryBy balancing the zero gradient moment on all axes, slices in two orthogonal planes may be spatially encoded simultaneously. The orthogonal slice groups may be acquired with equal or nonequal echo times.MethodsA Cartesian spoiled gradient echo simultaneous orthogonal plane imaging (SOPI) sequence was tested in phantom and in vivo. Multiplexed SOPI acquisitions were performed in which two parallel slices were imaged along two orthogonal axes simultaneously. An autocalibrating phase‐constrained 2D‐SENSE‐GRAPPA (generalized autocalibrating partially parallel acquisition) algorithm was implemented to reconstruct the multiplexed data.ResultsSOPI images without intraslice motion artifacts were reconstructed at a maximum frame rate of 8.16 Hz. The 2D‐SENSE‐GRAPPA reconstruction separated the parallel slices aliased along each orthogonal axis.ConclusionThe high spatiotemporal resolution provided by SOPI has the potential to be beneficial for intrafraction motion management during MR‐guided radiation therapy or other MRI‐guided interventions. Magn Reson Med 78:1700–1710, 2017. © 2016 International Society for Magnetic Resonance in Medicine.
Perspektivwechsel
Authors: Glorius Frank , Rakers Lena , Tebben Ludger 
Published: December 2016

AbstractÜber sechs Monate arbeiteten Kunststudierende und Chemiedoktoranden aus Münster zusammen in ihren Laboren und Werkstätten. Dabei tauschten sie nicht nur Techniken und Sichtweisen aus: Beide Seiten lernten auch neu, sich auszudrücken, und ließen sich voneinander inspirieren.
Mehr Pumpen, weniger Kosten
Authors: Melching Achim 
Published: December 2016

AbstractAn Gloveboxen entfernen meist ölgedichtete Drehschieberpumpen die Luft aus Haupt‐ und Transferkammer. Für einen sicheren Dauerbetrieb ist der Wartungsaufwand allerdings hoch. Das Institut für Anorganische Chemie und Kristallographie der Universität Bremen hat zusammen mit dem Pumpenhersteller Vacuubrand nach einer Lösung gesucht, welche die Kosten dafür senkt.
Bioanorganisches Symposium 2016 in Aachen
Authors: Liebhäuser Patricia 
Published: December 2016

AbstractAnfang September fand an der RWTH Aachen das Bioanorganische Symposium 2016 mit 90 Teilnehmern statt. Die Mitglieder der DFG‐geförderten Forschergruppe 1405 präsentierten in diesem Abschlusssymposium ihre neuesten Ergebnisse zum Thema Biological and Inorganic Charge Transfer Dynamics und diskutierten sie mit den neun eingeladenen Vortragenden aus aller Welt. Zur Forschergruppe gehören insgesamt sieben Arbeitsgruppen von der RWTH Aachen, der Universität Hamburg, dem Center of Free‐Electron Laser Science (CFEL) und der Universität Paderborn. Sie beschäftigt sich mit experimentellen und theoretischen Untersuchungen von Elektronen‐ und Charge‐Transfer‐Reaktionen. Etliche Wissenschaftler aus Deutschland nahmen teil und präsentierten neben 25 Vorträgen 55 Poster.
Vorstandssitzung
Authors: Koch Wolfram 
Published: December 2016

AbstractDie dritte Sitzung des Vorstands der Gesellschaft Deutscher Chemiker im Jahr 2016 fand am 9. September statt; wie alle zwei Jahre in Verbindung mit der Versammlung der Gesellschaft Deutscher Naturforscher und Ärzte, dieses Mal bei der 129. Versammlung in Greifswald.
Die lebendige Chemie
Authors: Lindhorst Thisbe K. 
Published: December 2016

AbstractIm Jahr 1867 gründete sich in Berlin die Deutsche Chemische Gesellschaft, die ältere der beiden Vorgängerorganisationen der Gesellschaft Deutscher Chemiker. Bevor es ab Januar 2017 losgeht mit dem Trubel des Jubiläumsjahres, sollten ein paar nachdenkliche Worte nicht fehlen.
An International Urogynecological Association (IUGA)/International Continence Society (ICS) joint report on the terminology for the conservative and nonpharmacological management of female pelvic floor dysfunction
Authors: Bo Kari , Frawley Helena C. , Haylen Bernard T. , Abramov Yoram , Almeida Fernando G. , Berghmans Bary , Bortolini Maria , Dumoulin Chantale , Gomes Mario , McClurg Doreen , Meijlink Jane , Shelly Elizabeth , Trabuco Emanuel , Walker Carolina , Wells Amanda 
Published: December 2016

Introduction and hypothesis There has been an increasing need for the terminology on the conservative management of female pelvic floor dysfunction to be collated in a clinically based consensus report.Methods This Report combines the input of members and elected nominees of the Standardization and Terminology Committees of two International Organizations, the International Urogynecological Association (IUGA) and the International Continence Society (ICS), assisted at intervals by many external referees. An extensive process of nine rounds of internal and external review was developed to exhaustively examine each definition, with decision‐making by collective opinion (consensus). Before opening up for comments on the webpages of ICS and IUGA, five experts from physiotherapy, neurology, urology, urogynecology, and nursing were invited to comment on the paper.Results A Terminology Report on the conservative management of female pelvic floor dysfunction, encompassing over 200 separate definitions, has been developed. It is clinically based, with the most common symptoms, signs, assessments, diagnoses, and treatments defined. Clarity and ease of use have been key aims to make it interpretable by practitioners and trainees in all the different specialty groups involved in female pelvic floor dysfunction. Ongoing review is not only anticipated, but will be required to keep the document updated and as widely acceptable as possible.Conclusion A consensus‐based terminology report for the conservative management of female pelvic floor dysfunction has been produced, aimed at being a significant aid to clinical practice and a stimulus for research.
Societal preferences in the treatment of pediatric medulloblastoma: Balancing risk of death and quality of life
Authors: Khakban Amir , Mohammadi Tima , Lynd Larry D. , Mabbott Don , Bouffet Eric , Gastonguay Louise , Zafari Zafar , Malkin David , Taylor Michael , Marra Carlo A. 
Published: December 2016

AbstractPurposeMedulloblastoma is the most prevalent childhood brain cancer. Children with medulloblastoma typically receive a combination of surgery, radiation, and chemotherapy. The survival rate is high but survivors often have sequelae from radiotherapy of the entire developing brain and spinal cord. Ongoing genetic studies have suggested that decreasing the dose of radiation might be possible among children with favorable molecular variants; however, this may result in an increased disease recurrence. As such, there is a need to investigate the nature of trade‐offs that individuals are willing to make regarding the treatment of medulloblastoma.MethodWe used best–worst scaling to estimate the importance of attributes affecting the general public's decision making around the treatment of medulloblastoma. After conducting focus groups, we selected three relevant attributes: (1) the accuracy of the genetic test; (2) the probability of serious adverse effects of the treatment(s); and (3) the survival rate. Using the paired method, we applied a conditional logit model to estimate preferences.ResultsIn total, 3,006 respondents (51.3% female) with an average age of 43 years answered the questionnaires. All coefficients were statistically significantly different from zero and the attribute levels of adverse effects and the survival rate had the most impact on individuals’ stated decision making.ConclusionOverall, respondents showed high sensitivity to children experiencing disability particularly in the setting of a good prognosis. However, among children with poor prognostic molecular variants, participants showed tolerance about having a child with mild and partial disability compared to a low rate of survival.
A combined registration and finite element analysis method for fast estimation of intraoperative brain shift; phantom and animal model study
Authors: Mohammadi Amrollah , Ahmadian Alireza , Rabbani Shahram , Fattahi Ehsan , Shirani Shapour 
Published: December 2016

AbstractBackgroundFinite element models for estimation of intraoperative brain shift suffer from huge computational cost. In these models, image registration and finite element analysis are two time‐consuming processes.MethodsThe proposed method is an improved version of our previously developed Finite Element Drift (FED) registration algorithm. In this work the registration process is combined with the finite element analysis. In the Combined FED (CFED), the deformation of whole brain mesh is iteratively calculated by geometrical extension of a local load vector which is computed by FED.ResultsWhile the processing time of the FED‐based method including registration and finite element analysis was about 70 s, the computation time of the CFED was about 3.2 s. The computational cost of CFED is almost 50% less than similar state of the art brain shift estimators based on finite element models.ConclusionsThe proposed combination of registration and structural analysis can make the calculation of brain deformation much faster.