bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mitochondrial Phosphatase PGAM5 Is Dispensable for Necroptosis but Promotes Inflammasome Activation in Macrophages
Authors: Moriwaki Kenta , Farias Luz Nivea , Balaji Sakthi , De Rosa Maria Jose , O’Donnell Carey L. , Gough Peter J. , Bertin John , Welsh Raymond M. , Chan Francis Ka-Ming 
Published: November 2015

Abstract
The cytokine IL-1β is intimately linked to many pathological inflammatory conditions. Mature IL-1β secretion requires cleavage by the inflammasome. Recent evidence indicates that many cell death signal adaptors have regulatory roles in inflammasome activity. These include the apoptosis inducers FADD and caspase 8, and the necroptosis kinases receptor interacting protein kinase 1 (RIPK1) and RIPK3. PGAM5 is a mitochondrial phosphatase that has been reported to function downstream of RIPK3 to promote necroptosis and IL-1β secretion. To interrogate the biological function of PGAM5, we generated Pgam5−/− mice. We found that Pgam5−/− mice were smaller compared with wild type littermates, and male Pgam5−/− mice were born at sub-Mendelian ratio. Despite these growth and survival defects, Pgam5−/− cells responded normally to multiple inducers of apoptosis and necroptosis. Rather, we found that PGAM5 is critical for IL-1β secretion in response to NLRP3 and AIM2 inflammasome agonists. Moreover, vesicular stomatosis virus–induced IL-1β secretion was impaired in Pgam5−/− bone marrow–derived macrophages, but not in Ripk3−/− bone marrow–derived dendritic cells, indicating that PGAM5 functions independent of RIPK3 to promote inflammasome activation. Mechanistically, PGAM5 promotes ASC polymerization, maintenance of mitochondrial integrity, and optimal reactive oxygen species production in response to inflammasome signals. Hence PGAM5 is a novel regulator of inflammasome and caspase 1 activity that functions independently of RIPK3.
Helios Controls a Limited Subset of Regulatory T Cell Functions
Authors: Sebastian Mathew , Lopez-Ocasio Maria , Metidji Amina , Rieder Sadiye Amcaoglu , Shevach Ethan M. , Thornton Angela M. 
Published: November 2015

Abstract
A subpopulation (60–70%) of Foxp3+ regulatory T cells (Tregs) in both mouse and man expresses the transcription factor Helios, but its role in Treg function is still unknown. We generated Treg-specific Helios-deficient mice to examine the function of Helios in Tregs. We show that the selective deletion of Helios in Tregs leads to slow, progressive systemic immune activation, hypergammaglobulinemia, and enhanced germinal center formation in the absence of organ-specific autoimmunity. Helios-deficient Treg suppressor function was normal in vitro, as well as in an in vivo inflammatory bowel disease model. However, Helios-deficient Tregs failed to control the expansion of pathogenic T cells derived from scurfy mice, failed to mediate T follicular regulatory cell function, and failed to control both T follicular helper cell and Th1 effector cell responses. In competitive settings, Helios-deficient Tregs, particularly effector Tregs, were at a disadvantage, indicating that Helios regulates effector Treg fitness. Thus, we demonstrate that Helios controls certain aspects of Treg-suppressive function, differentiation, and survival.
Blast overpressure induced axonal injury changes in rat brainstem and spinal cord
Authors: Kallakuri Srinivasu , Purkait Heena S. , Dalavayi Satya , VandeVord Pamela , Cavanaugh John M. 
Published: November 2015

ABSTRACT
Introduction: Blast induced neurotrauma has been the signature wound in returning soldiers from the ongoing wars in Iraq and Afghanistan. Of importance is understanding the pathomechansim(s) of blast overpressure (OP) induced axonal injury. Although several recent animal models of blast injury indicate the neuronal and axonal injury in various brain regions, animal studies related to axonal injury in the white matter (WM) tracts of cervical spinal cord are limited. Objective: The purpose of this study was to assess the extent of axonal injury in WM tracts of cervical spinal cord in male Sprague Dawley rats subjected to a single insult of blast OP. Materials and Methods: Sagittal brainstem sections and horizontal cervical spinal cord sections from blast and sham animals were stained by neurofilament light (NF-L) chain and beta amyloid precursor protein immunocytochemistry and observed for axonal injury changes. Results: Observations from this preliminary study demonstrate axonal injury changes in the form of prominent swellings, retraction bulbs, and putative signs of membrane disruptions in the brainstem and cervical spinal cord WM tracts of rats subjected to blast OP. Conclusions: Prominent axonal injury changes following the blast OP exposure in brainstem and cervical spinal WM tracts underscores the need for careful evaluation of blast induced injury changes and associated symptoms. NF-L immunocytochemistry can be considered as an additional tool to assess the blast OP induced axonal injury.
A pure non-gestational ovarian choriocarcinoma with delayed solitary brain metastases: Case report and review of the literature
Authors: Rao K.V.L Narasinga , Konar Subhas , Gangadharan Jagathlal , Vikas V. , Sampath S. 
Published: November 2015

ABSTRACTChoriocarcinoma is the most malignant tumour of gestational trophoblastic origin. Most ovarian choriocarcinomas are gestational in origin and usually metastasize to the ovary from uterine or tubal choriocarcinoma. Non gestational choriocarcinoma (NGOC) of the ovary is exceedingly rare and usually seen along with other germ cell tumors. Non gestational choriocarcinoma has been found to be resistant to single-agent chemotherapy and has a worse prognosis than gestational choriocarcinoma. We are reporting long term follow up of published rare case of pure non gestational ovarian choriocarcinoma (NGOC) with concurrent metastases to the spleen and adrenal glands, who developed a delayed solitary brain metastases, two years after completion of primary treatment. Surgery along with triple agent chemotherapy and radiotherapy was found to give good remission in this aggressive disease.
Rechtseenheid: concepten, motieven, actoren en instrumenten
Authors: Stip M.J.C. , Zijlstra S.E. 
Published: November 2015

Rechtseenheid: concepten, motieven, actoren en instrumenten
Rechtseenheid speelt in de juridische dogmatiek en de rechtsvorming een grote rol. Op tal van fronten wordt er onderzoek naar gedaan, beleid over ontwikkeld, en komt rechtseenheidbeogende wetgeving tot stand. Bij nadere bestudering blijkt het daarbij echter niet steeds over hetzelfde te gaan. In dit artikel wordt een typologie van varianten van rechtseenheid ontwikkeld. De auteurs analyseren het debat en trachten het te ontdoen van de begripsmatige verwarring. Vervolgens werken zij een van de varianten uit, namelijk rechtseenheid tussen rechtsnormen die niet logisch tegenstrijdig zijn. Daarna wordt besproken welke actoren en instrumenten een rol spelen bij het bewerkstelligen van die variant van rechtseenheid.
Therapeutic Efficacy of Fresh, Autologous Mesenchymal Stem Cells for Severe Refractory Gingivostomatitis in Cats
Authors: Arzi Boaz , Mills-Ko Emily , Verstraete Frank J.M. , Kol Amir , Walker Naomi J. , Badgley Megan R. , Fazel Nasim , Murphy William J. , Vapniarsky Natalia , Borjesson Dori L. 
Published: November 2015

Abstract

Mesenchymal stem cells (MSCs) are a promising therapy for immune-mediated and inflammatory disorders, because of their potent immunomodulatory properties. In this study, we investigated the use of fresh, autologous, adipose-derived MSCs (ASCs) for feline chronic gingivostomatitis (FCGS), a chronic, debilitating, idiopathic, oral mucosal inflammatory disease. Nine cats with refractory FCGS were enrolled in this pilot study. Each cat received 2 intravenous injections of 20 million autologous ASCs, 1 month apart. Oral biopsies were taken before and at 6 months after the first ASC injection. Blood immune cell subsets, serum protein, and cytokine levels were measured at 0, 1, 3, and 6 months after treatment to assess immunomodulatory effects. Seven of the 9 cats completed the study. Five cats responded to treatment by either complete clinical remission (n = 3) or substantial clinical improvement (n = 2). Two cats were nonresponders. Cats that responded to treatment also exhibited systemic immunomodulation demonstrated by decreased numbers of circulating CD8+ T cells, a normalization of the CD4/CD8 ratio, decreased neutrophil counts, and interferon-γ and interleukin (IL)-1β concentration, and a temporary increase in serum IL-6 and tumor necrosis factor-α concentration. No clinical recurrence has occurred following complete clinical remission (follow-up of 6–24 months). In this study, cats with &lt;15% cytotoxic CD8 T cells with low expression of CD8 (CD8lo) cells were 100% responsive to ASC therapy, whereas cats with &gt;15% CD8lo cells were nonresponders. The relative absence of CD8lo cells may be a biomarker to predict response to ASC therapy, and may shed light on pathogenesis of FCGS and mechanisms by which ASCs decrease oral inflammation and affect T-cell phenotype.

Significance
This study is the first to demonstrate the safety and efficacy of fresh, autologous, adipose-derived stem cell systemic therapy for a naturally occurring, chronic inflammatory disease in cats. The findings demonstrate that this therapy resulted in complete clinical and histological resolution or reduction in clinical disease severity and immune modulation in most cats. This study also identified a potentially useful biomarker that could dictate patient enrollment and shed light on immune modulation mechanism. As a naturally occurring animal model, FCGS also provides a strategic platform for potentially translatable therapy for the treatment of human oral inflammatory disease.
Splicing Express: a software suite for alternative splicing analysis using next-generation sequencing data
Authors: Kroll Jose E. , Kim Jihoon , Ohno-Machado Lucila , de Souza Sandro J. 
Published: November 2015

Motivation.Alternative splicing events (ASEs) are prevalent in the transcriptome of eukaryotic species and are known to influence many biological phenomena. The identification and quantification of these events are crucial for a better understanding of biological processes. Next-generation DNA sequencing technologies have allowed deep characterization of transcriptomes and made it possible to address these issues. ASEs analysis, however, represents a challenging task especially when many different samples need to be compared. Some popular tools for the analysis of ASEs are known to report thousands of events without annotations and/or graphical representations. A new tool for the identification and visualization of ASEs is here described, which can be used by biologists without a solid bioinformatics background.Results.A software suite namedSplicing Expresswas created to perform ASEs analysis from transcriptome sequencing data derived from next-generation DNA sequencing platforms. Its major goal is to serve the needs of biomedical researchers who do not have bioinformatics skills.Splicing Expressperforms automatic annotation of transcriptome data (GTF files) using gene coordinates available from the UCSC genome browser and allows the analysis of data from all available species. The identification of ASEs is done by a known algorithm previously implemented in another tool namedSplooce. As a final result,Splicing Expresscreates a set of HTML files composed of graphics and tables designed to describe the expression profile of ASEs among all analyzed samples. By using RNA-Seq data from the Illumina Human Body Map and the Rat Body Map, we show thatSplicing Expressis able to perform all tasks in a straightforward way, identifying well-known specific events.Availability and Implementation.Splicing Expressis written in Perl and is suitable to run only in UNIX-like systems. More details can be found at:http://www.bioinformatics-brazil.org/splicingexpress.
In‐hospital recurrent stroke in ipsilateral carotid web patients undergoing thrombectomy
Authors: Khan Farhan , Kala Narendra , Chang Kelvin , Shu Liqi , Goldstein Eric D. , Torabi Radmehr , Moldovan Krisztina , Jayaraman Mahesh , Mohammadzadeh Nahid , Furie Karen , Yaghi Shadi 
Published: August 2024

AbstractObjectiveCarotid artery web is a possible cause of ischemic stroke, especially in young patients who lack conventional risk factors. The immediate and long‐term outcomes are not well studied. We aimed to determine the association between an ipsilateral carotid web and in‐hospital stroke recurrence.MethodsWe analyzed data from adult patients admitted with an acute anterior circulation large vessel occlusion at a Comprehensive Stroke Center between July 2015 and March 2023. The primary outcome was in‐hospital stroke recurrence and secondary outcome was in‐hospital recurrent LVO. Multivariable logistic regression was performed to examine the association between ipsilateral carotid web and recurrent ischemic stroke and recurrent LVO.ResultsOf the 1463 patients with anterior circulation large vessel occlusion, 27 (1.8%) had an ipsilateral carotid artery web. Patients with carotid web were younger (median age (IQR), 60 years (53–67 years) versus 74 years (62–84 years), P < 0.01) and less likely to be Caucasian (60% vs. 80%, p = 0.014). Of the 27 patients with carotid web, 18 (70%) had no identifiable competing stroke mechanism. When compared to patients without ipsilateral carotid web, those with an ipsilateral carotid web had a higher risk of recurrent ischemic stroke (adjusted RR: 4.38, 95% CI: 1.38–13.85) and recurrent ipsilateral large vessel occlusion (adjusted RR: 4.49, 95% CI: 1.41–14.21).InterpretationCarotid webs are an under recognized cause of acute large vessel occlusion and are associated with higher risk of early recurrence. Studies are needed to validate our findings and test early revascularization strategies in patients with symptomatic carotid artery webs.
Adsorption of Typical VOCs Onto Ti2CO2 MXene with Implications in Early‐Stage Lung Cancer Diagnosis: A DFT Study
Authors: Chlikhy Youssef , Mazroui M'hammed 
Published: August 2024

AbstractIn this work, Ti2CO2 MXene is employed as a sensing material to detect volatile organic compounds (VOCs). Using Density Functional Theory (DFT) calculations, the adsorption properties of toluene, isopropanol, formaldehyde, and acetonitrile are calculated and compared. The electronic properties are analyzed to gain insight into the adsorption mechanism. Additionally, the recovery time and sensitivities are studied to evaluate the sensing performance of Ti2CO2 in detecting these VOCs. The results show that the four molecules undergo physisorption. Bader charge analysis shows a small charge transfer from the molecules to the MXene material. The adsorption of these molecules induces changes in the electronic properties of Ti2CO2, particularly in terms of resistance and work function. These changes are used to estimate the sensing response of this material toward these VOCs. Notably, the results highlight that Ti2CO2 exhibits good sensitivity and selectivity, especially in the case of isopropanol. These findings demonstrate the ability of Ti2CO2 as a sensing material for detecting VOCs for the early diagnosis of cancer.
MED12 Loss‐of‐Function Variants as a Cause of Congenital Diaphragmatic Hernia in Females With Hardikar Syndrome and Nonspecific Intellectual Disability
Authors: Kao Eric C. , Mizerik Elizabeth A. , Bacino Carlos A. , Dai Hongzheng , Vossaert Liesbeth , Scott Daryl A. 
Published: August 2024

ABSTRACTMediator complex subunit 12 (MED12) is required for the assembly of the kinase module of Mediator, a regulatory complex that controls the formation of the RNA polymerase II‐mediated preinitiation complex. MED12‐related disorders display unique gender‐specific genotype–phenotype associations and include X‐linked recessive Opitz–Kaveggia syndrome, Lujan–Fryns syndrome, Ohdo syndrome, and nonspecific intellectual disability in males predominantly carrying missense variants, and X‐linked dominant Hardikar syndrome and nonspecific intellectual disability in females known to predominantly carry de novo nonsense/frameshift and nonsense/missense variants, respectively. MED12 was previously identified as a low‐penetrance candidate gene for non‐isolated congenital diaphragmatic hernia (CDH+). At the time, however, there was insufficient evidence to confirm this association. In a clinical database search, we identified 18 individuals who were molecularly diagnosed with MED12‐related disorders by exome or genome sequencing, including eight missense, four frameshift, two nonsense, and one splice variant. Nine of these variants have not been previously reported. Two females with nonspecific intellectual disability were found to carry a de novo frameshift variant, indicating that potentially truncating variants causing nonspecific intellectual disability are not limited to nonsense variants. Notably, CDH was reported in three out of seven females with Hardikar syndrome or nonspecific intellectual disability but was not reported in males with MED12‐related disorders. These results suggest that pathogenic MED12 variants are a cause of CDH+ in females with Hardikar syndrome and nonspecific intellectual disability.
DNA Interaction, Molecular Docking, Antimicrobial, Anticancer and Thermal Studies of Ternary Metal Complexes of N‐Methylbenzylamine and Ethylenediamine
Authors: Kore Srinivas , Maddikayala Sravanthi , Bengi Kavitha , Pulimamidi Saritha Reddy 
Published: August 2024

ABSTRACTN‐Methylbenzylamine and ethylenediamine were utilized as primary and auxiliary ligands, respectively, alongside metal salts to synthesize ternary metal complexes: [FeCl3(Nmba)(en)], [CoCl2(Nmba)(en)(H2O)] and [CuCl2(Nmba)(en)(H2O)]. These complexes were thoroughly characterized using spectral and analytical techniques, revealing an octahedral geometry for all. Coats–Redfern calculations indicated their non‐spontaneous nature yet highlighted their thermal stability. DNA binding studies unveiled a groove binding mode for the complexes, with intrinsic binding constants (Kb) and Stern–Volmer quenching constant (Ksq) supporting their strong binding capabilities. Nuclease activity against pBR322 was assessed through gel electrophoresis. Additionally, docking studies using AutoDock 4.2 software provided insights into their binding affinities. In terms of biological activities, the Cu complex demonstrated superior cytotoxicity and antibacterial and antifungal properties compared to the other ternary metal complexes. This suggests its potential for further exploration in biomedical applications.
Preparation and photocatalytic mechanism of magnetic Ag2S/CoFe1.95Dy0.05O4 Z‐scheme heterojunction
Authors: Qingwang Liu , Mai Xu , Ying Meng 
Published: August 2024

AbstractThe synthesis of high‐efficiency magnetic composite photocatalyst by doping magnetic cobalt ferrite and compounding single semiconductor photocatalyst is a promising strategy to improve the oxidation ability of photocatalytic systems. In this paper, CoFe1.95Dy0.05O4 (CFDO) was prepared by doping Dy element into magnetic CoFe2O4, and Ag2S (AS)/CFDO with high‐efficiency magnetic photocatalyst was synthesized by compounding AS with CFDO as the substrate. The photocatalytic samples were characterized by different advanced characterization methods, and their photocatalytic degradation of methylene blue (MB) was studied. The results show that AS/CFDO exhibits higher visible light response, excellent photogenerated charge separation ability and migration efficiency, and excellent catalytic performance in the catalytic degradation system. The photocatalytic activity of AS/CFDO was the highest, and its photocatalytic degradation kinetic constant K was 2.48 and 1.54 times that of AS and CFDO, respectively. In addition, the catalyst contained in the catalytically contaminated solution can be effectively separated by an external magnetic field to achieve multiple cycles of degradation and recycling. The cyclic degradation experiments showed that AS/CFDO exhibited high degradation stability during the photodegradation process. After the fifth reuse, the degradation efficiency was still more than 88.0%. Finally, the possible photocatalytic mechanism of the samples was discussed. Therefore, this work provides an effective solution for the construction of photocatalysts with high efficiency, magnetic recovery, and cyclic degradation stability and avoids the secondary pollution of catalysts to organic wastewater. It is of great significance to create an environmentally friendly catalytic method for efficient cyclic degradation of organic wastewater.
How fast do scholarly papers get read by various user groups? A longitudinal and cross‐disciplinary analysis of the evolution of Mendeley readership
Authors: Fang Zhichao , Ho Chonkit , Han Zekun , Wu Puqing 
Published: August 2024

AbstractTo provide a dynamic perspective on the evolution of Mendeley readership, this study conducts an 8‐year longitudinal analysis of approximately 3.4 million scholarly papers published in 2015. Mendeley readership data were collected annually from 2016 to 2023 for the sampled papers to analyze the temporal accumulation patterns of readership following publication. The results indicate that Mendeley readership exhibits a speed advantage compared to citations and a prevalence advantage compared to Twitter mentions, demonstrating both initial prevalence and sustained growth on a yearly basis. However, the patterns of accumulation vary across disciplines, with papers in Biomedical and Health Sciences showing the fastest accrual of extensive Mendeley readership data. Leveraging demographic data provided by Mendeley, this study further investigates how different user groups—categorized by academic status, disciplinary affiliation, and geographic location—engage with papers across various disciplines. The findings highlight Mendeley readership as a rapid and substantial altmetric, yet they also emphasize the need to interpret the nature of the attention captured by Mendeley readership with caution, considering its potential biases introduced by the varying engagement levels of different user groups across disciplines.
Production of sweet potato distilled beverage: Economic evaluation via enzymatic and acid hydrolysis
Authors: Carvalho Débora Gonçalves , Trierweiler Luciane Ferreira , Trierweiler Jorge Otávio 
Published: August 2024

AbstractThis study aimed to assess the feasibility of utilizing sweet potato waste for distilled beverage production in two process scenarios: (i) acid hydrolysis and (ii) enzymatic hydrolysis. Laboratory experiments were conducted using in natura sweet potato waste and the data obtained were used to design a production system capable of producing 1000 L of distillate per day. Net present value (NPV), internal rate of return (IRR), and discounted payback indicators were used for economic analysis, with a minimum attractiveness rate (MARR) of 15%. The initial investment for the projects was BRL 1 139 610.86 (USD 223 146.80) for enzymatic hydrolysis and BRL 1 314 824.54 (USD 257 455.36) for acid hydrolysis. Packaging costs were identified as the greatest expenses in the process. Assuming a selling price of BRL 14.50 (USD 2.84), the enzymatic hydrolysis process demonstrated an NPV of BRL 4 792 520.77 (USD 938 421.92) and IRR of 83.39%, and the acid hydrolysis process showed an NPV of BRL 2 191 636.78 (USD 429 143.68) and IRR of 42.49%. Consequently, establishing a production plant for distilled beverages proved to be more economically attractive when employing the enzymatic hydrolysis process and fermentation simultaneously, resulting in a return on investment within 1.46 years of operation. Upon scrutinizing the gain matrix, it became apparent that critical factors with the potential to affect the project's feasibility detrimentally, if increased, included the cost of acquiring packaging, the final volumetric fraction of the distilled beverage, and the tax rate. Nevertheless, an upturn in the selling price of the beverage and a process improvement yield both have the potential to underpin the project effectively.