bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virology and epidemiology analyses of global adenovirus-associated conjunctivitis outbreaks, 1953–2013
Authors: ZHANG L. , ZHAO N. , SHA J. , WANG C. , JIN X. , AMER S. , LIU S. 
Published: January 2016

SUMMARYThis study aimed to compare the virology and epidemiology of epidemic keratoconjunctivitis (EKC), pharyngoconjunctival fever (PCF) and acute haemorrhagic conjunctivitis (AHC) outbreaks worldwide caused by the human adenovirus (HAdV) from 1953 to 2013. Eighty-three hexon sequences from 76 conjunctivitis outbreaks were analysed and subtyped using Mega 5.05, Clustal X and SimPlot software. Epidemiology was performed for the area, age and seasonal distribution. A phylogenetic analysis indicated that all the isolates could be divided into three subgenetic lineages, without a common ancestor. The major causes of the outbreaks were Ad8, Ad7 and Ad2 co-infection with enterovirus 70 (EV70) in EKC, PCF and AHC, respectively. The epidemiological findings suggested that EKC and AHC were circulating predominantly in Asia during the early winter and spring, whereas PCF was circulating mainly in China, Australia and the United States during the summer. This study suggests that EKC, AHC and PCF outbreaks have different circulating patterns throughout the world and are caused by different adenovirus serotypes. A global surveillance system should be established to monitor conjunctivitis outbreaks in the future.
Phenotypic and genotypic characterization of twoToxoplasma gondiiisolates in free-range chickens from Uberlândia, Brazil
Authors: LOPES C. S. , FRANCO P. S. , SILVA N. M. , SILVA D. A. O. , FERRO E. A. V. , PENA H. F. J. , SOARES R. M. , GENNARI S. M. , MINEO J. R. 
Published: January 2016

SUMMARYThe aim of this study was to determine the seroprevalence ofToxoplasma gondiiinfection in free-range chickens from Uberlândia, Minas Gerais state, Brazil, and characterize the genotypic and phenotypic features of two isolates of this parasite, considering the importance of these hosts in the epidemiology of toxoplasmosis. Serum samples from 108 free-range chickens were obtained from ten different districts, and submitted to the modified agglutination test (MAT) for the presence of anti-T. gondiiantibodies, and brain and heart tissue samples from infected chickens were processed for mouse bioassay. An overall seroprevalence of 71·3% was found and antibody titres ranged from 16 to 4096. After confirmation of seropositivity by mouse bioassay, the determination of theT. gondiigenotypes of two isolates was performed by PCR–RFLP, using primers for the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22–8, c29–2, L358, PK1, new SAG2, Apico and CS3. TheseT. gondiiisolates, designated TgChBrUD1and TgChBrUD2, were obtained from heart samples of free-range chickens. The TgChBrUD1 isolate belonged to ToxoDB PCR–RFLP genotype 11 and the TgChBrUD2 isolate belonged to ToxoDB PCR–RFLP genotype 6. Both isolates demonstrated high virulence in a rodent model, with the TgChBrUD1 isolate able to induce brain cysts, in accord with its pattern of multiplication rates in human fibroblast culture. Taken together, these results reveal high prevalence ofT. gondiiinfection in free-range chickens throughout Uberlândia, indicating an important degree of oocyst environmental contamination and the existence of considerable risk forT. gondiitransmission to humans by consumption of free-range chicken as a food source.
An assessment of the gravity signature of the Windmill Islands, East Antarctica
Authors: Bailey Brad T. , Morgan Peter J. , Lackie Mark A. 
Published: January 2016

AbstractA gravity survey was conducted on the Windmill Islands, East Antarctica, during the 2004–05 summer season. The aim of the study was to investigate the subsurface geology of the Windmill Islands area. Ninety-seven gravity stations were established. Additionally, 49 observations from a survey in 1993–94 were re-reduced and merged with the 2004–05 data. A three-dimensional subsurface model was constructed from the merged gravity dataset to determine the subsurface geology of the Windmill Islands. The main country rock in the Windmill Islands is a Garnet-bearing Granite Gneiss. A relatively dense intrusive charnockite unit, the Ardery Charnockite, generates the dominant gravity high of the study area and has been modelled to extend to depths of 7–13 km. It has moderate to steep contacts against the surrounding Garnet-bearing Granite Gneiss. The Ardery Charnockite surrounds a less dense granite pluton, the Ford Granite, which is modelled to a depth of 6–12 km and creates a localized gravity low. This granitic pluton extends at depth towards the east. The modelling process has also shown that Mitchell Peninsula is linked to the adjacent Law Dome ice cap by an ‘ice ramp’ of approximately 100 m thickness.
Effect of simulated rainfall during wheat seed development and maturation on subsequent seed longevity is reversible
Authors: Ellis Richard H. , Yadav Gajender 
Published: January 2016

AbstractPoor wheat seed quality in temperate regions is often ascribed to wet production environments. We investigated the possible effect of simulated rain during seed development and maturation on seed longevity in wheat (Triticum aestivum L.) cv. Tybalt grown in the field (2008, 2009) or a polythene tunnel house (2010). To mimic rain, the seed crops were wetted from above with the equivalent of 30 mm (2008, 2009) or 25 mm rainfall (2010) at different stages of seed development and maturation (17–58 DAA, days after 50% anthesis), samples were harvested serially, and subsequent air-dry seed longevity estimated. No pre-harvest sprouting occurred. Seed longevity (p50, 50% survival period in experimental hermetic storage at 40°C with c. 15% moisture content) in field-grown controls increased during seed development and maturation, attaining maxima at 37 (2008) or 44 DAA (2009); it declined thereafter. Immediate effects of simulated rain at 17–58 DAA in field studies (2008, 2009) on subsequent seed longevity were negative but small, e.g. a 1–4 d delay in seed quality improvement for treatments early in development, but with no damage detected at final harvests. In rainfall-protected conditions (2010), simulated rain close to harvest maturity (55–56 DAA) reduced longevity immediately and substantially, with greater damage from two sequential days of wetting than one; again, later harvests provided evidence of recovery in subsequent longevity. In the absence of pre-harvest sprouting, the potentially deleterious effects of rainfall to wheat seed crops on subsequent seed longevity may be reversible in full or in part.
A comparison of Geriatric Depression Scale scores in older Australian and Japanese women
Authors: Campbell K. E. , Dennerstein L. , Tacey M. , Fujise N. , Ikeda M. , Szoeke C. 
Published: January 2016

Aims:The aim of this study was to compare the prevalence of depressive symptoms in Australian and Japanese populations of community-dwelling older women using the Geriatric Depression Scale (GDS-15). In addition, the relationship between lifestyle and health factors and higher ratings of depressive symptoms was also examined to determine if there were culturally consistent risk factors associated with higher depressive symptom scores.Methods:A total of 444 community based women aged between 65 and 77 years completed a depressive symptom measure (GDS-15) and provided information on common lifestyle factors. The Australian sample (n = 222) were drawn from the Women's Healthy Ageing Project and the age-matched, Japanese sample from the Kumamoto Ageing Study of Mental Health (n = 222). The GDS was chosen to; (1) reduce the impact of physical symptoms associated with old age and, (2) reduce the inflation in scores that may result from the Japanese tendency to endorse somatic items more often than Western adults.Results:Mean GDS total scores were significantly higher for the Japanese population 3.97 ± 3.69 compared with 1.73 ± 2.7 for Australian women. The percentages of women scoring in the normal; mild and moderate ranges for depression were 91, 7 and 2% for Australia and 67, 24 and 9% for Japan. Scores remained significantly higher for the Japanese cohort when controlling for lifestyle and health factors associated with depression. The analysis of lifestyle and health characteristics showed that the greatest difference between cohorts was in the area of living status, with more Australian women living with their partner and more than three times as many Japanese women living with their children. When the data for the countries was considered independently employment status affected the likelihood of higher depression scores in the Australian sample while heart disease and poor sleep impacted the risk for the Japanese population.Conclusions:Significantly more Japanese women scored within the mild and moderate ranges on the GDS compared with their Australian peers, even when controlling for possible confounding factors. Of the lifestyle and health factors assessed in this analysis no single variable was a common risk factor for higher depressive scores for both countries. The presence of cultural influences that may impact the risk of experiencing depressive symptoms, and culture specific patterns of item endorsement on depressive symptom measures, needs to be explored in more detail.
A shear gradient-activated microfluidic device for automated monitoring of whole blood haemostasis and platelet function
Authors: Jain Abhishek , Graveline Amanda , Waterhouse Anna , Vernet Andyna , Flaumenhaft Robert , Ingber Donald E. 
Published: January 2016

AbstractAccurate assessment of blood haemostasis is essential for the management of patients who use extracorporeal devices, receive anticoagulation therapy or experience coagulopathies. However, current monitoring devices do not measure effects of haemodynamic forces that contribute significantly to platelet function and thrombus formation. Here we describe a microfluidic device that mimics a network of stenosed arteriolar vessels, permitting evaluation of blood clotting within small sample volumes under pathophysiological flow. By applying a clotting time analysis based on a phenomenological mathematical model of thrombus formation, coagulation and platelet function can be accurately measured in vitro in patient blood samples. When the device is integrated into an extracorporeal circuit in pig endotoxemia or heparin therapy models, it produces real-time readouts of alterations in coagulation ex vivo that are more reliable than standard clotting assays. Thus, this disposable device may be useful for personalized diagnostics and for real-time surveillance of antithrombotic therapy in clinic.
Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping
Authors: Freedman Kevin J. , Otto Lauren M. , Ivanov Aleksandar P. , Barik Avijit , Oh Sang-Hyun , Edel Joshua B. 
Published: January 2016

AbstractSingle-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and capture is predominantly diffusion limited. This problem is compounded when taking into account the capture volume of a nanopore, typically 108–1010 times smaller than the sample volume. To rectify this disproportionate ratio, we demonstrate a simple, yet powerful, method based on coupling single-molecule dielectrophoretic trapping to nanopore sensing. We show that DNA can be captured from a controllable, but typically much larger, volume and concentrated at the tip of a metallic nanopore. This enables the detection of single molecules at concentrations as low as 5 fM, which is approximately a 103 reduction in the limit of detection compared with existing methods, while still maintaining efficient throughput.
Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6
Authors: Guye Patrick , Ebrahimkhani Mohammad R. , Kipniss Nathan , Velazquez Jeremy J. , Schoenfeld Eldi , Kiani Samira , Griffith Linda G. , Weiss Ron 
Published: January 2016

AbstractHuman induced pluripotent stem cells (hiPSCs) have potential for personalized and regenerative medicine. While most of the methods using these cells have focused on deriving homogenous populations of specialized cells, there has been modest success in producing hiPSC-derived organotypic tissues or organoids. Here we present a novel approach for generating and then co-differentiating hiPSC-derived progenitors. With a genetically engineered pulse of GATA-binding protein 6 (GATA6) expression, we initiate rapid emergence of all three germ layers as a complex function of GATA6 expression levels and tissue context. Within 2 weeks we obtain a complex tissue that recapitulates early developmental processes and exhibits a liver bud-like phenotype, including haematopoietic and stromal cells as well as a neuronal niche. Collectively, our approach demonstrates derivation of complex tissues from hiPSCs using a single autologous hiPSCs as source and generates a range of stromal cells that co-develop with parenchymal cells to form tissues.
Three-dimensional controlled growth of monodisperse sub-50 nm heterogeneous nanocrystals
Authors: Liu Deming , Xu Xiaoxue , Du Yi , Qin Xian , Zhang Yuhai , Ma Chenshuo , Wen Shihui , Ren Wei , Goldys Ewa M. , Piper James A. , Dou Shixue , Liu Xiaogang , Jin Dayong 
Published: January 2016

AbstractThe ultimate frontier in nanomaterials engineering is to realize their composition control with atomic scale precision to enable fabrication of nanoparticles with desirable size, shape and surface properties. Such control becomes even more useful when growing hybrid nanocrystals designed to integrate multiple functionalities. Here we report achieving such degree of control in a family of rare-earth-doped nanomaterials. We experimentally verify the co-existence and different roles of oleate anions (OA−) and molecules (OAH) in the crystal formation. We identify that the control over the ratio of OA− to OAH can be used to directionally inhibit, promote or etch the crystallographic facets of the nanoparticles. This control enables selective grafting of shells with complex morphologies grown over nanocrystal cores, thus allowing the fabrication of a diverse library of monodisperse sub-50 nm nanoparticles. With such programmable additive and subtractive engineering a variety of three-dimensional shapes can be implemented using a bottom–up scalable approach.
Genome-wide association study identifies variation at 6q25.1 associated with survival in multiple myeloma
Authors: Johnson David C. , Weinhold Niels , Mitchell Jonathan S. , Chen Bowang , Kaiser Martin , Begum Dil B. , Hillengass Jens , Bertsch Uta , Gregory Walter A. , Cairns David , Jackson Graham H. , Försti Asta , Nickel Jolanta , Hoffmann Per , Nöethen Markus M. , Stephens Owen W. , Barlogie Bart , Davis Faith E. , Hemminki Kari , Goldschmidt Hartmut , Houlston Richard S. , Morgan Gareth J. 
Published: January 2016

AbstractSurvival following a diagnosis of multiple myeloma (MM) varies between patients and some of these differences may be a consequence of inherited genetic variation. In this study, to identify genetic markers associated with MM overall survival (MM-OS), we conduct a meta-analysis of four patient series of European ancestry, totalling 3,256 patients with 1,200 MM-associated deaths. Each series is genotyped for ∼600,000 single nucleotide polymorphisms across the genome; genotypes for six million common variants are imputed using 1000 Genomes Project and UK10K as the reference. The association between genotype and OS is assessed by Cox proportional hazards model adjusting for age, sex, International staging system and treatment. We identify a locus at 6q25.1 marked by rs12374648 associated with MM-OS (hazard ratio=1.34, 95% confidence interval=1.22–1.48, P=4.69 × 10–9). Our findings have potential clinical implications since they demonstrate that inherited genotypes can provide prognostic information in addition to conventional tumor acquired prognostic factors.
Negative magnetoresistance in Dirac semimetal Cd3As2
Authors: Li Hui , He Hongtao , Lu Hai-Zhou , Zhang Huachen , Liu Hongchao , Ma Rong , Fan Zhiyong , Shen Shun-Qing , Wang Jiannong 
Published: January 2016

AbstractA large negative magnetoresistance (NMR) is anticipated in topological semimetals in parallel magnetic fields, demonstrating the chiral anomaly, a long-sought high-energy-physics effect, in solid-state systems. Recent experiments reveal that the Dirac semimetal Cd3As2 has the record-high mobility and positive linear magnetoresistance in perpendicular magnetic fields. However, the NMR has not yet been unveiled. Here we report the observation of NMR in Cd3As2 microribbons in parallel magnetic fields up to 66% at 50 K and visible at room temperatures. The NMR is sensitive to the angle between magnetic and electrical fields, robust against temperature and dependent on the carrier density. The large NMR results from low carrier densities in our Cd3As2 samples, ranging from 3.0 × 1017 cm−3 at 300 K to 2.2 × 1016 cm−3 below 50 K. We therefore attribute the observed NMR to the chiral anomaly. In perpendicular magnetic fields, a positive linear magnetoresistance up to 1,670% at 14 T and 2 K is also observed.
Interplay of active processes modulates tension and drives phase transition in self-renewing, motor-driven cytoskeletal networks
Authors: Mak Michael , Zaman Muhammad H. , Kamm Roger D. , Kim Taeyoon 
Published: January 2016

AbstractThe actin cytoskeleton—a complex, nonequilibrium network consisting of filaments, actin-crosslinking proteins (ACPs) and motors—confers cell structure and functionality, from migration to morphogenesis. While the core components are recognized, much less is understood about the behaviour of the integrated, disordered and internally active system with interdependent mechano-chemical component properties. Here we use a Brownian dynamics model that incorporates key and realistic features—specifically actin turnover, ACP (un)binding and motor walking—to reveal the nature and underlying regulatory mechanisms of overarching cytoskeletal states. We generate multi-dimensional maps that show the ratio in activity of these microscopic elements determines diverse global stress profiles and the induction of nonequilibrium morphological phase transition from homogeneous to aggregated networks. In particular, actin turnover dynamics plays a prominent role in tuning stress levels and stabilizing homogeneous morphologies in crosslinked, motor-driven networks. The consequence is versatile functionality, from dynamic steady-state prestress to large, pulsed constrictions.
Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm
Authors: El Khatib Mariam , Martins Alexandre , Bourgeois Dominique , Colletier Jacques-Philippe , Adam Virgile 
Published: January 2016

AbstractPhototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by hybridisation of Superfolder-GFP with rsEGFP2 and inherited the fast folding properties of the former together with the rapid switching of the latter, but at the cost of a reduced switching contrast. Structural characterisation of the switching mechanisms of rsFolder and rsEGFP2 revealed different scenarios for chromophore cis-trans isomerisation and allowed designing rsFolder2, a variant of rsFolder that exhibits improved switching contrast and is amenable to RESOLFT nanoscopy. The rsFolders can be efficiently expressed in the E. coli periplasm, opening the door to the nanoscale investigation of proteins localised in hitherto non-observable cellular compartments.
Diversity and interactions of microbial functional genes under differing environmental conditions: insights from a membrane bioreactor and an oxidation ditch
Authors: Xia Yu , Hu Man , Wen Xianghua , Wang Xiaohui , Yang Yunfeng , Zhou Jizhong 
Published: January 2016

AbstractThe effect of environmental conditions on the diversity and interactions of microbial communities has caused tremendous interest in microbial ecology. Here, we found that with identical influents but differing operational parameters (mainly mixed liquor suspended solid (MLSS) concentrations, solid retention time (SRT) and dissolved oxygen (DO) concentrations), two full-scale municipal wastewater treatment systems applying oxidation ditch (OD) and membrane bioreactor (MBR) processes harbored a majority of shared genes (87.2%) but had different overall functional gene structures as revealed by two datasets of 12-day time-series generated by a functional gene array-GeoChip 4.2. Association networks of core carbon, nitrogen and phosphorus cycling genes in each system based on random matrix theory (RMT) showed different topological properties and the MBR nodes showed an indication of higher connectivity. MLSS and DO were shown to be effective in shaping functional gene structures of the systems by statistical analyses. Higher MLSS concentrations resulting in decreased resource availability of the MBR system were thought to promote positive interactions of important functional genes. Together, these findings show the differences of functional potentials of some bioprocesses caused by differing environmental conditions and suggest that higher stress of resource limitation increased positive gene interactions in the MBR system.
The Influence of Doping on the Optoelectronic Properties of PbS Colloidal Quantum Dot Solids
Authors: Papagiorgis P. , Stavrinadis A. , Othonos A. , Konstantatos G. , Itskos G. 
Published: January 2016

AbstractWe report on an extensive spectroscopic investigation of the impact of substitutional doping on the optoelectronic properties of PbS colloidal quantum dot (CQD) solids. N-doping is provided by Bi incorporation during CQD synthesis as well as post-synthetically via cation exchange reactions. The spectroscopic data indicate a systematic quenching of the excitonic absorption and luminescence and the appearance of two dopant-induced contributions at lower energies to the CQD free exciton. Temperature-dependent photoluminescence indicates the presence of temperature-activated detrapping and trapping processes of photoexcitations for the films doped during and after synthesis, respectively. The data are consistent with a preferential incorporation of the dopants at the QDs surface in the case of the cation-exchange treated films versus a more uniform doping profile in the case of in-situ Bi incorporation during synthesis. Time-resolved experiments indicate the presence of fast dopant- and excitation-dependent recombination channels attributed to Auger recombination of negatively charged excitons, formed due to excess of dopant electrons. The data indicate that apart from dopant compensation and filling of dopant induced trap states, a fraction of the Bi ionized electrons feeds the QD core states resulting in n-doping of the semiconductor, confirming reported work on devices based on such doped CQD material.