bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of Long Non‐Coding RNA MEG3 Inhibits Proliferation of Hepatocellular Carcinoma Huh7 Cells via Negative Modulation of miRNA‐664
Authors: He Jin‐Hua , Han Ze‐Ping , Liu Ji‐Ming , Zhou Jia‐Bin , Zou Mao‐Xian , Lv Yu‐Bing , Li Yu‐Guang , Cao Ming‐Rong 
Published: April 2017

ABSTRACTEvidence is accumulating that long non‐coding RNAs (lncRNAs) are involved in human tumorigenesis and dysregulated in many cancers, including hepatocellular carcinoma (HCC). Because lncRNAs can regulate essential pathways that contribute to tumor initiation and progression with their tissue specificity, lncRNAs are valuable biomarkers and therapeutic targets. Maternally expressed gene 3 (MEG3) is a lncRNA overexpressed in HCC cells that inhibits HCC progression, however, the mechanism remains largely unknown. Recently, a novel regulatory mechanism has been proposed in which RNAs can cross‐talk with each other via competing for shared microRNAs (miRNAs). The proposed competitive endogenous RNAs could mediate the bioavailability of miRNAs on their targets, thus imposing another level of post‐transcriptional regulation. In the current study, we demonstrated that MEG3 is down‐regulated in HCC tissues. MEG3 over‐expression imposes another level of post‐transcriptional regulation, whereas MEG3 overexpression increase the expression of the miR‐664 target gene, ADH4, through competitive “sponging” miR‐664. In addition, NF‐κB may affect transcription of MEG3 by directly binding to the promoter region. Our data revealed that NF‐κB may affect the transcript of MEG3. MEG3 overexpression inhibited the proliferation of HCC cells, at least in part by affecting miR‐664mediated regulation of ADH4. Together, these results suggest that MEG3 is a suppressor of tumor which acts in part through “sponging” miR‐664. J. Cell. Biochem. 118: 3713–3721, 2017. © 2017 Wiley Periodicals, Inc.
Liposomes as a Putative Tool to Investigate NAADP Signaling in Vasculogenesis
Authors: Di Nezza Francesca , Zuccolo Estella , Poletto Valentina , Rosti Vittorio , De Luca Antonio , Moccia Francesco , Guerra Germano , Ambrosone Luigi 
Published: April 2017

ABSTRACTNicotinic acid adenine dinucleotide phosphate (NAADP) is the newest discovered intracellular second messengers, which is able to release Ca2+ stored within endolysosomal (EL) vesicles. NAADP‐induced Ca2+ signals mediate a growing number of cellular functions, ranging from proliferation to muscle contraction and differentiation. Recently, NAADP has recently been shown to regulate angiogenesis by promoting endothelial cell growth. It is, however, still unknown whether NAADP stimulates proliferation also in endothelial progenitor cells, which are mobilized in circulation after an ischemic insult to induce tissue revascularization. Herein, we described a novel approach to prepare NAADP‐containing liposomes, which are highly cell membrane permeable and are therefore amenable for stimulating cell activity. Accordingly, NAADP‐containing liposomes evoked an increase in intracellular Ca2+ concentration, which was inhibited by NED‐19, a selective inhibitor of NAADP‐induced Ca2+ release. Furthermore, NAADP‐containing liposomes promoted EPC proliferation, a process which was inhibited by NED‐19 and BAPTA, a membrane permeable intracellular Ca2+ buffer. Therefore, NAADP‐containing liposomes stand out as a promising tool to promote revascularization of hypoxic/ischemic tissues by favoring EPC proliferation. J. Cell. Biochem. 118: 3722–3729, 2017. © 2017 Wiley Periodicals, Inc.
All‐Trans Retinoic Acid Ameliorates Arsenic‐Induced Oxidative Stress and Apoptosis in the Rat Uterus by Modulating MAPK Signaling Proteins
Authors: Chatterjee Aniruddha , Chatterji Urmi 
Published: April 2017

ABSTRACTExposure to arsenic leads to inhibition of the anti‐oxidant defense mechanism of the body. Reactive oxygen species generated in response to arsenic causes reproductive failures in exposed females and also acts as an inducer of apoptosis. As a prospective remedial agent, all‐trans retinoic acid (ATRA) was assessed for reversing arsenic‐induced oxidative stress and apoptosis. Rats exposed to arsenic for 28 days were allowed to recover naturally or were treated simultaneously with ATRA for 28 days or up to 56 days. Production of H2O2 was detected using 2′,7′‐dichlorfluorescein diacetate (DCFCA) by flow cytometry. Catalase, superoxide dismutase, glutathione, ALT, and AST were estimated by biochemical assays and Western blot analyses. Detection of apoptosis was performed using annexin V‐FITC/propidium iodide. Expressions of p53, p21, cleaved caspase 3, JNK/pJNK, and ERK/pERK levels were estimated using Western blot analysis. Elemental arsenic deposition in the rat uterus and liver was estimated by atomic absorption spectrophotometry. Our results confirmed that ATRA ameliorated sodium arsenite‐induced ROS generation, restored redox balance, and prevented apoptosis. Concomitant recovery was observed to be more prominent for ATRA‐treated rats as compared to the rats that were allowed to recover naturally for 56 days. Tissue arsenic deposition was significantly reduced in the uterus upon continuous ATRA treatment. The results revealed that ATRA reversed arsenic‐induced free radical generation, activated the anti‐oxidant defence system, and subsequently repressed p53‐dependent apoptosis through inhibition of the MAPK signaling components. J. Cell. Biochem. 118: 3796–3809, 2017. © 2017 Wiley Periodicals, Inc.
A quantitative method for the determination of bosutinib in human plasma using high‐performance liquid chromatography and ultraviolet detection
Authors: Sumimoto Takahiro , Nakahara Ryosuke , Sato Yuhki , Itoh Hiroki 
Published: April 2017

BackgroundWe propose a simple, sensitive, and fast high‐performance liquid chromatography ultraviolet detection (HPLC‐UV) method for the quantitative determination of bosutinib in human plasma.MethodsPlasma samples were processed using an Oasis hydrophilic‐lipophilic balance extraction cartridge (1 mL, 30 mg). Bosutinib and the internal standard imatinib were separated using a mobile phase of 0.5% Na2PO4H2O (pH 3.5)‐acetonitrile‐methanol (55:25:20, v/v/v) on a CAPCELL PAK C18 MG II reversed‐phase column 250 nm×4.6 nm i.d., at a flow rate of 1.0 mL/min, with ultraviolet detection at 250 nm.ResultsThe calibration curve exhibited linearity over the bosutinib concentration range of 25‐1500 ng/mL at 250 nm, with coefficient of variation for intraday precision of 2.42%, 6.04%, and 1.11% for 100, 250, and 1500 ng/mL, respectively, of bosutinib. The lower limit of detection was 20 ng/mL. The extraction recovery rates for bosutinib ranged from 84.36% to 85.82%. The intra‐ and interday precision was below 8.7%, and the accuracy ranged from −5.95% to 5.85% over the linear range. No notable matrix effects or astaticism were observed.ConclusionThe proposed HPLC‐UV method was successfully applied as an assay to detect bosutinib in human plasma.
Impact of microRNA‐375 and its target gene SMAD‐7 polymorphism on susceptibility of colorectal cancer
Authors: Shaker Olfat Gamil , Mohammed Shereen Rashad , Mohammed Asmaa Mohammed , Mahmoud Zeinab 
Published: April 2017

BackgroundColorectal cancer (CRC) has a high morbidity and mortality. Many studies reported that mir‐375 is frequently down‐regulated in many cancers including esophageal cancer, hepatocellular carcinoma, breast cancer and leukemias.AimOur aim was to study the expression of microRNA‐375 and its target gene SMAD‐7 polymorphisms (rs4939827) in CRC patients in comparison to control subjects and to correlate these results with clinical data of patients to elucidate their role in pathogenesis and early diagnosis of CRC.Material and methodsThe present study was conducted on 122 subjects divided into 86 patients with CRC and 36 age‐ and sex‐matched controls. The followings were done to all subjects: full history taking, full clinical examination, complete blood picture, serum (ALT, AST), serum albumin, CEA, TLC, PLT, and creatinine. Gene expression of miRNA‐375 from serum was done by real‐time PCR. Gene polymorphism SNPs of SMAD7 (rs4939827) was also done in DNA extracted from blood by real‐time PCR.ResultsAs regards the polymorphism of SMAD7, we found that CC (wild) genotype has high percentage in controls compared to CRC cases (36.1% vs 15.1%). Meanwhile, the mutant and heterozygotes genotypes showed high percentage among cases compared to controls (33.7%, and 51.2% respectively) vs (22.2%, and 41.7% respectively) with no significant statistical analysis. There was a statistically significant high T‐allelic frequency among cases and C‐allelic frequency among controls. There was a statistically significant association between fold change in micro RNA (‐375) and the susceptibility to CRC as there is down‐regulation of the microRNA‐375 in CRC group with fold change in 0.42±0.27.ConclusionMicro RNA‐375 and rs4939827 SNP in SMAD7 could be considered as potential markers for detecting and early diagnosing CRC patients.
RHD alleles among pregnant women with serologic discrepant weak D phenotypes from a multiethnic population and risk of alloimmunization
Authors: Bub Carolina Bonet , Aravechia Maria Giselda , Costa Thiago Henrique , Kutner José Mauro , Castilho Lilian 
Published: April 2017

BackgroundA considerable number of RHD alleles responsible for weak and partial D phenotypes have been identified. Serologic determination of these phenotypes is often doubtful and makes genetic analysis of RHD gene highly desirable in transfusion recipients and pregnant women. We analyzed the RHD gene in a cohort of pregnant women with doubtful D phenotypes.MethodsRHD genotyping was performed on 104 cases with D typing discrepancies or with history of serologic weak D phenotype. Laboratory‐developed DNA tests, RHD BeadChip (Bioarray Solutions, Immucor), and sequencing were used to identify the RHD alleles.ResultsMolecular analyses showed 23 of 104 (22%) pregnant women were RHD*weak D types 1, 2, or 3 and not at risk for anti‐D. Fifty‐one (49%) were RHD*weak partial 4.0, 6 RHD*weak D type 38 (6%), 1 RHD*weak D type 45 (1%), 1 RHD*weak D type 67 (1%), and potentially at risk for being alloimmunized and making anti‐D. Partial D was identified in 22 of 104 (21%) patients and definitively at risk for anti‐D.DiscussionAppropriate classification of RhD phenotypes is recommended for correct indication of RhIG in pregnant women. However, the serologic distinction between RhD‐negative and RhD‐positive phenotypes is a difficult task in the case of D variants due to the variations in serologic testing. Our results show a great variability in RHD variant alleles in pregnant women from this population of high admixture. According to these results, 78% of these obstetric patients are at risk for anti‐D and candidates for RhIG.
Synthesis and Biological Activity of Amide Compounds Containing Pyrazole Mandelic Acid Groups
Authors: Xie Yan , Ruan Xiang‐Hui , Gong Hua‐Yu , Wang Yi‐Hui , Wang Xiao‐Bin , Zhang Ju‐Ping , Li Qin , Xue Wei 
Published: April 2017

A series of novel mandelic acid pyrazole amide compounds were synthesized and characterized. Moreover, their antiviral activities against tobacco mosaic virus were evaluated. The bioassay results indicated that as‐prepared compounds showed antiviral activity against tobacco mosaic virus at a concentration of 500 g/mL. The curative activity of compound 4g is 59.5%, which was comparable with positive control (Ningnanmycin, 61.0%). The inactivation activity of the compound 4l exhibited 80.1%, which was higher than that of Ningnanmycin (75.0%), and its protective activity was 88.7%, which is comparable with that of Ningnanmycin (90.7%).
Instantaneous and Selective Bare Eye Detection of Inorganic Fluoride Ion by Coumarin–Pyrazole‐Based Receptors
Authors: Jain Anshu , Gupta Ragini , Agarwal Madhu 
Published: April 2017

A series of rationally designed coumarin–pyrazole‐based scaffolds, equipped with N―H and C―H hydrogen bond donors (R1–R5) and containing various electron‐withdrawing groups at key positions, are synthesized and characterized in order to investigate their inorganic fluoride binding properties in highly competitive media (1:1 DMSO–water). Only one, 3‐{4‐[(2,4‐dinitrophenyl)‐hydrazonomethyl]‐1‐phenyl‐1H‐pyrazol‐3‐yl}‐chromen‐2‐one (R1), of the five compounds synthesized, is found to be capable to selectively detect inorganic fluoride via naked eye amongst other anionic species in aqueous media. Qualitative and spectroscopic studies exhibit that receptor R1 has the potential of showing instantaneous change of color from yellow to pink upon addition of sodium fluoride (0.95 ppm) in aqueous media, at concentration lower than that recommended by World Health Organization (1 ppm). Intensity of color increases with increasing fluoride concentration till 5 ppm, beyond which intensity of color change becomes saturated. This has established the applicability of this receptor for assessment of the level of fluoride in water. Anion binding studies carried out by UV–visible titration portrayed substantial bathochromic peak shift from 410 to 495 nm, upon addition of varying concentrations of aqueous sodium fluoride solution, which has validated the color change. Jobs plot data confirmed 1:1 stoichiometry between R1 and fluoride ion. 1H‐NMR investigation reveals that the deprotonation of N―H hydrogen donor group of receptor R1 and its interaction with fluoride ion is responsible for the observed color change.
Simultaneous determination of 106 pesticides in nuts by LC–MS/MS using freeze‐out combined with dispersive solid‐phase extraction purification
Authors: Wang Jingjing , Gong Zhiguo , Zhang Tingting , Feng Shun , Wang Jide , Zhang Yi 
Published: April 2017

As a result of the low water content and high fat matrices in nuts, it is very difficult to simultaneously determine multi‐pesticides in trace levels. Here, a sample pretreatment method was developed in which, microwave‐assisted solvent extraction was firstly used to extract pesticides, and then a two‐step cleanup method was conducted combining freeze‐out with dispersive solid‐phase extraction to remove the lipidic matrix. By this way, 106 pesticides were simultaneously determined in the complicated nut sample by using an ultra‐high pressure liquid chromatography coupled with a tandem mass spectrometer. Average recoveries were 75.3–119.3% with relative standard deviations < 14% at three concentration levels. The limits of detection and quantification were in the ranges of 0.3–3.0 and 1.0–10.0 μg/kg, respectively. Furthermore, the method was successfully applied to the determination of pesticides in 180 commercial nut samples.
Social distance between local residents and African–American expatriates in the context of Ghana's slavery‐based heritage tourism
Authors: Yankholmes Aaron , Timothy Dallen J. 
Published: April 2017

AbstractThis paper explores the social distance between local residents and African–Americans who have settled in Ghana since the 1960s. Data generated from in‐depth interviews suggest the African–American expatriates felt their proximity to collective slave memory or particularly slavery heritage conferred on them certain rights to exclude local residents who are more susceptible to forgetting the past. By appropriating traces of the past, the African–American expatriates provide a range of tourism services, albeit to visitors they believed subscribed to socially constructed meanings elicited at slave sites. The study suggests explicit recognition of African–American expatriates in the levels of contestations that result from slavery‐based heritage tourism.
Survey and analysis design for wood turtle population monitoring
Authors: Brown Donald J. , Cochrane Madaline M. , Moen Ron A. 
Published: April 2017

ABSTRACTPopulation monitoring is a fundamental component of wildlife management, and is necessary to track site‐ and regional‐level status and recovery of species of conservation concern. The wood turtle (Glyptemys insculpta) is a species of conservation concern for federal and state agencies because of population declines across the species’ range. We developed and tested a survey and analysis design to assist agencies in the Upper Midwest, USA, with establishment of long‐term monitoring programs for wood turtle populations. In spring of 2016, we conducted 8 replicate population surveys at 8 candidate long‐term monitoring sites in northeastern Minnesota, USA. Using field survey data and simulation models, we assessed the influence of distance from river surveyed, number of survey replications, and number of sites on abundance estimates; we also delineated important survey covariates and compared demographic estimates based on distance from river surveyed. We estimated site‐level abundances and compared survey designs using a multinomialN‐mixture model that included a removal sampling observation process. Mean abundance estimates were similar when surveying 2 transects (i.e., the river‐land interface to ∼25 m inland) or 4 transects (i.e., the river‐land interface to ∼55 m inland), but decreasing the survey distance from river reduced the precision of estimates. Mean abundance estimates were similar with ≥6 replications. Air temperature was an important predictor of survey‐specific detection probability, with maximum detectability at 19−23°C. Sex ratio and mean carapace length did not differ based on whether we surveyed 2 or 4 transects, and percentage of individuals by size class was nearly identical between the sampling designs. Simulations indicated that 75% of mean abundance estimates were within ±8% of true abundance when ≥15 sites were surveyed. The wood turtle survey and analysis design we developed and tested was effective for estimating abundance of wood turtle populations in northeastern Minnesota, and we encourage its use as a template for wood turtle monitoring programs in the Upper Midwest. © 2017 The Wildlife Society.
Self‐Organization of Hyperbranched Polyesters Functionalized with Pyrrolo[2,1‐a]isoquinoline End Groups and Their Fluorescent Recognition of Anthracene and Pyrene
Authors: Zeng Fanyang , Jiang Yuliang , Wang Bingxiang , Mao Chun , Han Qiaorong , Ma Zhenye 
Published: April 2017

The structurally controllable hyperbranched aromatic–aliphatic copolyesters HBPE‐MPICB4 and HBPE‐MPICB2 are synthesized by incorporating the periphery of a second‐generation hyperbranched polyester (HBPE) with methylpyrrolo[2,1‐a]isoquinoline‐1‐carbonitrile(E)‐4‐oxo‐[2‐(oxo‐λ3‐methyl)hydrazinyl]but‐2‐enoate (MPICB) groups via one‐pot synthesis. Organized supramolecular structures are self‐assembled in different organic media. Lamellar structure with a length of around 10 µm can be obtained in ethyl acetate. However, the identical HBPE‐MPICB4 and HBPE‐MPICB2 building blocks can self‐organize into flower‐like and vesicular structure in ethanol, with particle sizes around 12 and 5 µm, respectively. The noncovalent interactions of HBPE‐MPICB4 and HBPE‐MPICB2, that is, hydrogen bonding and π–π stacking, are critical for the construction of organized structures. Furthermore, host–guest recognitions by HBPE‐MPICB4 and HBPE‐MPICB2 are investigated by fluorescence studies. The results indicate that HBPE‐MPICB4 microflowers respond selectively to anthracene and pyrene through π–π stacking interactions.
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A unified method for boundedness in fully parabolic chemotaxis systems with signal‐dependent sensitivity
Authors: Mizukami Masaaki , Yokota Tomomi 
Published: April 2017

AbstractThis paper deals with the Keller–Segel system
urn:x-wiley:0025584X:media:mana201600399:mana201600399-math-0001where Ω is a bounded domain in with smooth boundary , ; χ is a nonnegative function satisfying for some and . In the case that and , Fujie established global existence of bounded solutions under the condition . On the other hand, when , Winkler asserted global existence of bounded solutions for arbitrary . However, there is a gap in the proof. Recently, Fujie tried modifying the proof; nevertheless it also has a gap. It seems to be difficult to show global existence of bounded solutions for arbitrary . Moreover, the condition for K when cannot connect with the condition when . The purpose of the present paper is to obtain global existence and boundedness under more natural and proper condition for χ and to build a mathematical bridge between the cases and .
Cost‐effectiveness of focused ultrasound, radiosurgery, and DBS for essential tremor
Authors: Ravikumar Vinod K. , Parker Jonathon J. , Hornbeck Traci S. , Santini Veronica E. , Pauly Kim Butts , Wintermark Max , Ghanouni Pejman , Stein Sherman C. , Halpern Casey H. 
Published: April 2017

AbstractBackgroundEssential tremor remains a very common yet medically refractory condition. A recent phase 3 study demonstrated that magnetic resonance‐guided focused ultrasound thalamotomy significantly improved upper limb tremor. The objectives of this study were to assess this novel therapy's cost‐effectiveness compared with existing procedural options.MethodsLiterature searches of magnetic resonance‐guided focused ultrasound thalamotomy, DBS, and stereotactic radiosurgery for essential tremor were performed. Pre‐ and postoperative tremor‐related disability scores were collected from 32 studies involving 83 magnetic resonance‐guided focused ultrasound thalamotomies, 615 DBSs, and 260 stereotactic radiosurgery cases. Utility, defined as quality of life and derived from percent change in functional disability, was calculated; Medicare reimbursement was employed as a proxy for societal cost. Medicare reimbursement rates are not established for magnetic resonance‐guided focused ultrasound thalamotomy for essential tremor; therefore, reimbursements were estimated to be approximately equivalent to stereotactic radiosurgery to assess a cost threshold. A decision analysis model was constructed to examine the most cost‐effective option for essential tremor, implementing meta‐analytic techniques.ResultsMagnetic resonance‐guided focused ultrasound thalamotomy resulted in significantly higher utility scores compared with DBS (P <0.001) or stereotactic radiosurgery (P <0.001). Projected costs of magnetic resonance‐guided focused ultrasound thalamotomy were significantly less than DBS (P <0.001), but not significantly different from radiosurgery.ConclusionsMagnetic resonance‐guided focused ultrasound thalamotomy is cost‐effective for tremor compared with DBS and stereotactic radiosurgery and more effective than both. Even if longer follow‐up finds changes in effectiveness or costs, focused ultrasound thalamotomy will likely remain competitive with both alternatives. © 2017 International Parkinson and Movement Disorder Society
The development and validation of static and adaptive screeners to measure the severity of panic disorder, social anxiety disorder, and obsessive compulsive disorder
Authors: Sunderland Matthew , Batterham Philip J. , Calear Alison L. , Carragher Natacha 
Published: April 2017

AbstractA series of static and adaptive screeners for panic disorder, social anxiety disorder (SAD), and obsessive compulsive disorder (OCD) were developed and compared using data‐driven methods to facilitate the measurement of each disorder in community samples. Data comprised 3175 respondents for the development sample and 3755 respondents for the validation sample, recruited independently using Facebook advertising. Item Response Theory (IRT) was utilized to develop static continuous screeners and to simulate computerized adaptive algorithms. The screeners consisted of a small subset of items from each bank (79% reduction in items for panic disorder, 85% reduction in items for SAD, and 84% reduction in items for OCD) that provided similar scores (r = 0.88–0.96). Both static and adaptive screeners were valid with respect to existing scales that purportedly measure similar constructs (r > 0.70 for panic disorder, r > 0.76 for SAD, and r > 0.68 for OCD). The adaptive scales were able to maintain a higher level of precision in comparison to the static scales and evidenced slightly higher concordance with scores generated by the full item banks. The screeners for panic disorder, SAD, and OCD could be used as a flexible approach to measure and monitor the severity of psychopathology in tailored treatment protocols.