bluuebunny/crossref_metadata_2025_split huggingface dataset:
Approach of automatic 3D geological mapping: the case of the Kovdor phoscorite-carbonatite complex, NW Russia
Authors: Kalashnikov A. O. , Ivanyuk G. Yu , Mikhailova J. A. , Sokharev V. A. 
Published: July 2017

AbstractWe have developed an approach for automatic 3D geological mapping based on conversion of chemical composition of rocks to mineral composition by logical computation. It allows to calculate mineral composition based on bulk rock chemistry, interpolate the mineral composition in the same way as chemical composition, and, finally, build a 3D geological model. The approach was developed for the Kovdor phoscorite-carbonatite complex containing the Kovdor baddeleyite-apatite-magnetite deposit. We used 4 bulk rock chemistry analyses – Femagn, P2O5, CO2 and SiO2. We used four techniques for prediction of rock types – calculation of normative mineral compositions (norms), multiple regression, artificial neural network and developed by logical evaluation. The two latter became the best. As a result, we distinguished 14 types of phoscorites (forsterite-apatite-magnetite-carbonate rock), carbonatite and host rocks. The results show good convergence with our petrographical studies of the deposit, and recent manually built maps. The proposed approach can be used as a tool of a deposit genesis reconstruction and preliminary geometallurgical modelling.
Towards Picogram Detection of Superparamagnetic Iron-Oxide Particles Using a Gradiometric Receive Coil
Authors: Graeser Matthias , Knopp Tobias , Szwargulski Patryk , Friedrich Thomas , von Gladiss Anselm , Kaul Michael , Krishnan Kannan M , Ittrich Harald , Adam Gerhard , Buzug Thorsten M. 
Published: July 2017

AbstractSuperparamagnetic iron-oxide nanoparticles can be used in medical applications like vascular or targeted imaging. Magnetic particle imaging (MPI) is a promising tomographic imaging technique that allows visualizing the 3D nanoparticle distribution concentration in a non-invasive manner. The two main strengths of MPI are high temporal resolution and high sensitivity. While the first has been proven in the assessment of dynamic processes like cardiac imaging, it is unknown how far the detection limit of MPI can be lowered. Within this work, we will present a highly sensitive gradiometric receive-coil unit combined with a noise-matching network tailored for the imaging of mice. The setup is capable of detecting 5 ng of iron in-vitro with an acquisition time of 2.14 sec. In terms of iron concentration we are able to detect 156 μg/L marking the lowest value that has been reported for an MPI scanner so far. In-vivo MPI mouse images of a 512 ng bolus and a 21.5 ms acquisition time allow for capturing the flow of an intravenously injected tracer through the heart of a mouse. Since it has been rather difficult to compare detection limits across MPI publications we propose guidelines to improve the comparability of future MPI studies.
PLA2R binds to the annexin A2-S100A10 complex in human podocytes
Authors: Fresquet Maryline , Jowitt Thomas A. , McKenzie Edward A. , Ball Matthew D. , Randles Michael J. , Lennon Rachel , Brenchley Paul E. 
Published: July 2017

AbstractPhospholipase A2 receptor (PLA2R) is a member of the mannose receptor family found in podocytes in human kidney. PLA2R is the target of the autoimmune disease, membranous nephropathy, characterised by production of anti-PLA2R autoantibodies which bind to the podocyte. However the function of PLA2R in health and in disease remains unclear. To gain insight into the molecular mechanisms of PLA2R function, we searched for its endogenous binding partners. Proteomic analysis identified annexinA2 as a potential interactor with the extracellular domains of PLA2R. We confirmed that PLA2R binds to annexinA2-S100A10 (A2t) complex with specific high affinity to the S100A10 component. The binding occured within the PLA2R NC3 fragment and was increased in acidic pH. Furthermore Ca2+ promoted the association of the PLA2R-A2t complex with phospholipid membranes in vitro. Within the podocyte, all three proteins were enriched in the plasma membrane and organelle membrane compartments. PLA2R co-localised with S100A10 at the cell surface and in extracellular vesicles. This novel interaction between PLA2R and the A2t complex offers insights into the role of PLA2R in podocytes and how autoantibodies might disrupt PLA2R function. The ability of podocytes to secrete vesicles containing PLA2R provides a route for engagement of PLA2R with the immune system.
Interface-induced spontaneous positive and conventional negative exchange bias effects in bilayer La0.7Sr0.3MnO3/Eu0.45Sr0.55MnO3 heterostructures
Authors: Murthy J. Krishna , Kumar P. S. Anil 
Published: July 2017

AbstractWe report zero-field-cooled spontaneous-positive and field-cooled conventional-negative exchange bias effects in epitaxial bilayer composed of La0.7Sr0.3MnO3 (LSMO) with ferromagnetic (FM) and Eu0.45Sr0.55MnO3 (ESMO) with A-type antiferromagnetic (AF) heterostructures respectively. A temperature dependent magnetization study of LSMO/ESMO bilayers grown on SrTiO3 (001) manifest FM ordering (TC) of LSMO at ~320 K, charge/orbital ordering of ESMO at ~194 K and AF ordering (TN) of ESMO at ~150 K. The random field Ising model has demonstrated an interesting observation of inverse dependence of exchange bias effect on AF layer thickness due to the competition between FM-AF interface coupling and AF domain wall energy. The isothermally field induced unidirectional exchange anisotropy formed at the interface of FM-LSMO layer and the kinetically phase-arrested magnetic phase obtained from the metamagnetic AF-ESMO layer could be responsible for the spontaneous exchange bias effect. Importantly, no magnetic poling is needed, as necessary for the applications. The FM-AF interface exchange interaction has been ascribed to the AF coupling with $$\sum {J}_{ex}\vec{{S}_{{\rm{FM}}}}\cdot \vec{{S}_{{\rm{AF}}}}$$
∑

J

e
x

S

FM

⃗

⋅

S

AF

⃗

($${J}_{ex}\approx {J}_{AF}$$

J

e
x

≈

J

A
F

, coupling constant between AF spins) for the spontaneous positive hysteresis loop shift, and the field-cooled conventional exchange bias has been attributed to the ferromagnetically exchanged interface with $${J}_{ex}\approx {J}_{F}$$

J

e
x

≈

J

F

(coupling constant between FM spins).
Plant survival and keystone pollinator species in stochastic coextinction models: role of intrinsic dependence on animal-pollination
Authors: Traveset Anna , Tur Cristina , Eguíluz Víctor M. 
Published: July 2017

AbstractCoextinction models are useful to understand community robustness to species loss and resilience to disturbances. We simulated pollinator extinctions in pollination networks by using a hybrid model that combined a recently developed stochastic coextinction model (SCM) for plant extinctions and a topological model (TCM) for animal extinctions. Our model accounted for variation in interaction strengths and included empirical estimates of plant dependence on pollinators to set seeds. The stochastic nature of such model allowed us determining plant survival to single (and multiple) extinction events, and identifying which pollinators (keystone species) were more likely to trigger secondary extinctions. Consistently across three different pollinator removal sequences, plant robustness was lower than in a pure TCM, and plant survival was more determined by dependence on the mutualism than by interaction strength. As expected, highly connected and dependent plants were the most sensitive to pollinator loss and collapsed faster in extinction cascades. We predict that the relationship between dependence and plant connectivity is crucial to determine network robustness to interaction loss. Finally, we showed that honeybees and several beetles were keystone species in our communities. This information is of great value to foresee consequences of pollinator losses facing current global change and to identify target species for effective conservation.
Elevated levels of the stress hormone, corticosterone, cause ‘pessimistic’ judgment bias in broiler chickens
Authors: Iyasere Oluwaseun S. , Beard Andrew P. , Guy Jonathan H. , Bateson Melissa 
Published: July 2017

AbstractPessimistic judgment biases, whereby humans or non-human animals interpret ambiguous information negatively, are hypothesised to be one of the suite of adaptive changes that comprise the vertebrate stress response. To test this hypothesis, we asked whether experimentally elevating levels of the glucocorticoid stress hormone, corticosterone, in broiler chickens produced a pessimistic judgment bias. We trained young chickens to discriminate a stimulus (paper cone) placed at two locations in an arena, one associated with reward (mealworms) and one with punishment (air puff). During seven days of non-invasive administration of either corticosterone or vehicle control, we tested the birds’ responses to the cone placed at ambiguous locations between the trained locations. Corticosterone-treated birds were more likely than controls to respond as if punishment was likely when the cone was placed near to the punished location. The degree of this ‘pessimism’ was associated with smaller relative spleen weight, which is a documented consequence of chronic stress in chickens. We conclude that changes in corticosterone levels in chickens are sufficient to cause a specific change in decision making, dubbed ‘pessimism’, whereby corticosterone-treated birds showed an increased expectation of punishment in the face of ambiguous information. Pessimism could be a useful welfare indicator in chickens.
Durability and Self-healing Effects of Hydrogel Coatings with respect to Contact Condition
Authors: Kim Chang-Lae , Kim Dae-Eun 
Published: July 2017

AbstractThe self-healing property of a hydrogel applied to a glass substrate as a thin polymer coating was assessed. The motivation was to develop a durable hydrogel coating that may be used to protect the surface of precision components from surface damage and scratches. The intrinsic swelling behavior of hydrogel fibers when they are exposed to moisture was exploited to attain the self-healing effect. The mechanical and self-healing properties of the dehydrated hydrogel coating by the freeze-drying process and the hydrated hydrogel coating that was reconstituted by the addition of water were analyzed. After conducting sliding tests with different loads and sliding distances, the wear area was hydrated with water to successfully induce self-healing of the hydrogel coating. It was also found that both the dehydrated hydrogel coating and the hydrated hydrogel coating had improved friction characteristics. In particular, the hydrated hydrogel coating had a much higher durability than the dehydrated coating.
Homocysteine induces mitochondrial dysfunction involving the crosstalk between oxidative stress and mitochondrial pSTAT3 in rat ischemic brain
Authors: Chen Shuang , Dong Zhiping , Zhao Yaqian , Sai Na , Wang Xuan , Liu Huan , Huang Guowei , Zhang Xumei 
Published: July 2017

AbstractHomocysteine (Hcy) has been shown to have a neurotoxic effect on ischemic brain cells; however, the underlying mechanisms remain incompletely understood. Here, we examined whether Hcy treatment influences mitochondria injury, oxidative stress, and mitochondrial STAT3 (mitoStat3) expression in rat ischemic brain. Our results demonstrated that Hcy treatment aggravated the damage of mitochondrial ultrastructure in the brain cortex and the dentate gyrus region of the hippocampus after focal cerebral ischemia. An elevated Hcy level was also accompanied by the significant inhibition of mitochondrial complex I–III enzymatic activities in addition to an increase in cytochrome c release. 8-Hydroxy-2′-deoxyguanosine (8-OHdG) content and mitoStat3 protein phosphorylation level were increased in Hcy-treated animals, whereas AG490, a Jak2 inhibitor, inhibited mitoStat3 phosphorylation as well as 8-OHdG levels induced by Hcy. In vitro studies revealed that Hcy also markedly increased reactive oxygen species (ROS) and mitoStat3 levels. In addition, the inhibition of pSTAT3 reduced Hcy-mediated increase in ROS levels, whereas quenching ROS using the ROS inhibitor glutathione ethyl ester inhibited Hcy-mediated pSTAT3 overactivation in Neuro2a cells. These findings suggest that the development of therapies that interfere with the ROS/pSTAT3 pathway may be helpful for treating cerebral infarction-related diseases associated with Hcy.
Associations between subjective well-being and subcortical brain volumes
Authors: Van ‘t Ent D. , den Braber A. , Baselmans B. M. L. , Brouwer R. M. , Dolan C. V. , Hulshoff Pol H. E. , de Geus E. J. C. , Bartels M. 
Published: July 2017

AbstractTo study the underpinnings of individual differences in subjective well-being (SWB), we tested for associations of SWB with subcortical brain volumes in a dataset of 724 twins and siblings. For significant SWB-brain associations we probed for causal pathways using Mendelian Randomization (MR) and estimated genetic and environmental contributions from twin modeling. Another independent measure of genetic correlation was obtained from linkage disequilibrium (LD) score regression on published genome-wide association summary statistics. Our results indicated associations of SWB with hippocampal volumes but not with volumes of the basal ganglia, thalamus, amygdala, or nucleus accumbens. The SWB-hippocampus relations were nonlinear and characterized by lower SWB in subjects with relatively smaller hippocampal volumes compared to subjects with medium and higher hippocampal volumes. MR provided no evidence for an SWB to hippocampal volume or hippocampal volume to SWB pathway. This was in line with twin modeling and LD-score regression results which indicated non-significant genetic correlations. We conclude that low SWB is associated with smaller hippocampal volume, but that genes are not very important in this relationship. Instead other etiological factors, such as exposure to stress and stress hormones, may exert detrimental effects on SWB and the hippocampus to bring about the observed association.
ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis
Authors: Soriano-Castell David , Chavero Albert , Rentero Carles , Bosch Marta , Vidal-Quadras Maite , Pol Albert , Enrich Carlos , Tebar Francesc 
Published: July 2017

AbstractClathrin-dependent and -independent pathways contribute for β1-integrin endocytosis. This study defines a tubular membrane clathrin-independent endocytic network, induced with the calmodulin inhibitor W13, for β1-integrin internalization. This pathway is dependent on increased phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) levels and dynamin activity at the plasma membrane. Exogenous addition of PI(4,5)P2 or phosphatidylinositol-4-phosphate 5-kinase (PIP5K) expression mimicked W13-generated-tubules which are inhibited by active Rac1. Therefore, the molecular mechanisms downstream of Rac1, that controls this plasma membrane tubulation, were analyzed biochemically and by the expression of different Rac1 mutants. The results indicate that phospholipase C and ROCK1 are the main Rac1 effectors that impair plasma membrane invagination and tubule formation, essentially by decreasing PI(4,5)P2 levels and promoting cortical actomyosin assembly respectively. Interestingly, among the plethora of proteins that participate in membrane remodeling, this study revealed that ROCK1, the well-known downstream RhoA effector, has an important role in Rac1 regulation of actomyosin at the cell cortex. This study provides new insights into Rac1 functioning on plasma membrane dynamics combining phosphatidylinositides and cytoskeleton regulation.
Effects of pulpotomy using mineral trioxide aggregate on prostaglandin transporter and receptors in rat molars
Authors: Ohkura Naoto , Edanami Naoki , Takeuchi Ryosuke , Tohma Aiko , Ohkura Mariko , Yoshiba Nagako , Yoshiba Kunihiko , Ida-Yonemochi Hiroko , Ohshima Hayato , Okiji Takashi , Noiri Yuichiro 
Published: July 2017

AbstractMineral trioxide aggregate (MTA) is a commonly used dental pulp-capping material with known effects in promoting reparative dentinogenesis. However, the mechanism by which MTA induces dentine repair remains unclear. The aim of the present study was to investigate the role of prostaglandin E2(PGE2) in dentine repair by examining the localisation and mRNA expression levels of its transporter (Pgt) and two of its receptors (Ep2 and Ep4) in a rat model of pulpotomy with MTA capping. Ep2 expression was detected in odontoblasts, endothelial cells, and nerve fibres in normal and pulpotomised tissues, whereas Pgt and Ep4 were immunolocalised only in the odontoblasts. Moreover, mRNA expression ofSlco2a1(encoding Pgt),Ptger2(encoding Ep2), andPtger4(encoding Ep4) was significantly upregulated in pulpotomised dental pulp and trigeminal ganglia after MTA capping. Our results provide insights into the functions of PGE2via Pgt and Ep receptors in the healing dentine/pulp complex and may be helpful in developing new therapeutic targets for dental disease.
Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression
Authors: Sebastiani Guido , Ventriglia Giuliana , Stabilini Angela , Socci Carlo , Morsiani Cristina , Laurenzi Andrea , Nigi Laura , Formichi Caterina , Mfarrej Bechara , Petrelli Alessandra , Fousteri Georgia , Brusko Todd M. , Dotta Francesco , Battaglia Manuela 
Published: July 2017

AbstractAutoimmune type 1 diabetes (T1D) is thought to be caused by a defective immune regulation with regulatory T (Treg) cells playing a fundamental role in this process. Tolerance mechanisms depend on tunable responses that are sensitive to minor perturbations in the expression of molecules that can be carried out by multiple epigenetic mechanisms, including regulation by microRNAs. In this study, microRNA expression profile was investigated in Treg cells isolated from peripheral blood (PB) and from pancreatic draining lymph nodes (PLN) of T1D patients and non-diabetic subjects. Among 72 microRNAs analyzed, miR-125a-5p resulted specifically hyper-expressed in Treg cells purified from PLN of T1D patients. TNFR2 and CCR2 were identified as miR-125a-5p target genes. Elevated miR-125a-5p was detected in Treg cells isolated from PLN but not from PB of donors with T1D and was associated with reduced CCR2 expression. A specific beta-cell expression of the CCR2-ligand (CCL2) was observed in the pancreata of cadaveric donors, suggesting that beta-cells are prone to attract CCR2+ Treg cells. These novel data propose a mechanism, occurring in PLNs of T1D patients, involving increased expression of miR-125a-5p on Treg cells which results into reduced expression of CCR2, thus limiting their migration and eventual function in the pancreas.
The advantages of endophyte-infected over uninfected tall fescue in the growth and pathogen resistance are counteracted by elevated CO2
Authors: Chen Wei , Liu Hui , Wurihan  , Gao Yubao , Card Stuart D. , Ren Anzhi 
Published: July 2017

AbstractAtmospheric CO2 concentrations are predicted to double within the next century. Despite this trend, the extent and mechanisms through which elevated CO2 affects grass-endophyte symbionts remain uncertain. In the present study, the growth, chemical composition and pathogen resistance of endophyte-infected (E+) and uninfected (E−) tall fescue were compared under elevated CO2 conditions. The results showed that the effect of endophyte infection on the growth of tall fescue was significantly affected by elevated CO2. Significant advantage of E+ over E− tall fescue in tiller number, maximum net photosynthetic rate and shoot biomass occurred only under ambient CO2. With CO2 concentration elevated, the beneficial effect of endophyte infection on the growth disappeared. Similarly, endophyte infection reduced lesion number and spore concentration of Curvularia lunata only under ambient CO2. These results suggest that the beneficial effect of endophyte infection on the growth and pathogen resistance of tall fescue could be counteracted by elevated CO2. An explanation for the counteraction may be found in a change in photosynthesis and nutritive quality of leaf tissue.
A novel pharmacodynamic assay to evaluate the effects of crystallization inhibitors on calcium phosphate crystallization in human plasma
Authors: Ferrer M. D. , Pérez M. M. , Cànaves M. M. , Buades J. M. , Salcedo C. , Perelló J. 
Published: July 2017

AbstractCardiovascular calcification (CVC) is a progressive complication of chronic kidney disease and a predictor of CV events and mortality. The use of biomarkers to predict CV risk and activities of potential or current treatment drugs in these patients could have a crucial impact on therapeutic approaches. Our aim was to develop a novel assay for measurement of the rate of calcium phosphate crystallization in human plasma and provide a tool to evaluate the effects of crystallization inhibitors. The efficacy of inhibitors was determined by adding inhibitory compounds (polyphosphates, fetuin-A, sodium thiosulfate or citrate) to control samples. The assay was additionally validated for SNF472, an experimental formulation of phytate being developed for the treatment of calciphylaxis and CVC in patients with end-stage renal disease (ESRD) undergoing hemodialysis (HD). The method was repeatable and reproducible. The plasma crystallization rate was reduced up to 80% in a concentration-dependent manner following treatment with inhibitors in vitro, among which SNF472 was the most potent. This method appears beneficial in evaluating and discriminating between inhibitory activities of compounds such as polyphosphates on calcium phosphate crystallization, which present a novel therapeutic approach to treat CVC in ESRD patients.
Restricted regions of enhanced growth of Antarctic krill in the circumpolar Southern Ocean
Authors: Murphy Eugene J. , Thorpe Sally E. , Tarling Geraint A. , Watkins Jonathan L. , Fielding Sophie , Underwood Philip 
Published: July 2017

AbstractFood webs in high-latitude oceans are dominated by relatively few species. Future ocean and sea-ice changes affecting the distribution of such species will impact the structure and functioning of whole ecosystems. Antarctic krill (Euphausia superba) is a key species in Southern Ocean food webs, but there is little understanding of the factors influencing its success throughout much of the ocean. The capacity of a habitat to maintain growth will be crucial and here we use an empirical relationship of growth rate to assess seasonal spatial variability. Over much of the ocean, potential for growth is limited, with three restricted oceanic regions where seasonal conditions permit high growth rates, and only a few areas around the Scotia Sea and Antarctic Peninsula suitable for growth of the largest krill (>60 mm). Our study demonstrates that projections of impacts of future change need to account for spatial and seasonal variability of key ecological processes within ocean ecosystems.
Electronic structure and its external electric field modulation of PbPdO2 ultrathin slabs with (002) and (211) preferred orientations
Authors: Yang Yanmin , Zhong Kehua , Xu Guigui , Zhang Jian-Min , Huang Zhigao 
Published: July 2017

AbstractThe Electronic structure of PbPdO2 with (002) and (211) preferred orientations were investigated using first-principles calculation. The calculated results indicate that, (002) and (211) orientations exhibit different electric field dependence of band-gap and carrier concentration. The small band gap and more sensitive electric field modulation of band gap were found in (002) orientation. Moreover, the electric field modulation of the resistivity up to 3–4 orders of magnitude is also observed in (002) slab, which reveals that origin of colossal electroresistance. Lastly, electric field modulation of band gap is well explained. This work should be significant for repeating the colossal electroresistance.