bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety and efficacy of allogeneic hematopoietic stem cell transplant after PD-1 blockade in relapsed/refractory lymphoma
Authors: Merryman Reid W. , Kim Haesook T. , Zinzani Pier Luigi , Carlo-Stella Carmelo , Ansell Stephen M. , Perales Miguel-Angel , Avigdor Abraham , Halwani Ahmad S. , Houot Roch , Marchand Tony , Dhedin Nathalie , Lescaut Willy , Thiebaut-Bertrand Anne , François Sylvie , Stamatoullas-Bastard Aspasia , Rohrlich Pierre-Simon , Labussière Wallet Hélène , Castagna Luca , Santoro Armando , Bachanova Veronika , Bresler Scott C. , Srivastava Amitabh , Kim Harim , Pesek Emily , Chammas Marie , Reynolds Carol , Ho Vincent T. , Antin Joseph H. , Ritz Jerome , Soiffer Robert J. , Armand Philippe 
Published: January 2017

Key Points
HSCT after PD-1 blockade is feasible, although may be associated with increased early immune toxicity. PD-1 blockade may cause persistent depletion of PD1+ T cells and alterations in T-cell differentiation impacting subsequent treatment.
The dangers of parathyroid biopsy
Authors: Kim Joanne , Horowitz Gilad , Hong Michael , Orsini Mario , Asa Sylvia L. , Higgins Kevin 
Published: January 2017

Background We report an unusual case of a 66-year-old female with a suspicious thoracic outlet mass presenting with severe biochemical hyperparathyroidism and classic hypercalcemic symptoms of renal and bone involvement. Case Presentation There was clinical suspicion for parathyroid carcinoma, further supported by intra-operative findings. However, the final pathology described a primary hyperceullar parathyroid lesion with pathognomonic changes secondary to fine-needle aspiration (FNA) biopsy, along with a separate parathyroid lesion likely resulting from seeding along the needle tract. Upon further review, record of a remote FNA was discovered. This case highlights the complications associated with parathyroid FNA resulting in a diagnostic challenge and raising the possibility of malignancy. Conclusions We therefore recommend to take caution when there is a prior parathyroid FNA, as it can present with the risks of a secondary lesion from seeding and increase resemblance of malignancy both clinically and through pathologic diagnosis.
Smell of heritage: a framework for the identification, analysis and archival of historic odours
Authors: Bembibre Cecilia , Strlič Matija 
Published: January 2017

Abstract
We don’t know much about the smells of the past. Yet, odours play an important role in our daily lives: they affect us emotionally, psychologically and physically, and influence the way we engage with history. Can this lead us to consider certain smells as cultural heritage? And if so, what would be the processes for the identification, protection and conservation of those heritage smells? In order to answer these questions, the connection between olfaction and heritage was approached in three ways: (1) through theoretical analysis of the concept and role of olfaction in heritage guidelines, leading to identification of places and practices where smell is fundamental to their identity, (2) through exploration of the evidence for use of smells in heritage as a tool to communicate with audiences; and (3) through experimental evaluation of the techniques and methods for analysing and archiving the smells, therefore enabling their documentation and preservation. We present this through the framework of Significance Assessment—Chemical Analysis—Sensory Analysis—Archiving. The smell of historic paper was chosen as the case study, based on its well-recognized cultural significance and available research. Odour characterization was achieved by collecting visitor descriptions of a historic book extract through a survey, and by conducting a sensory evaluation at a historic library. These were combined with the chemical information on the VOCs sampled from both a historic book and a historic library, to create the Historic Book Odour Wheel, a novel documentation tool representing the first step towards documenting and archiving historic smells.
Deficiency of N-myristoylation reveals calcineurin activity as regulator of IFN-γ-producing γδ T cells
Authors: Rampoldi Francesca , Brunk Fabian , Bonrouhi Mahnaz , Federico Giuseppina , Krunic Damir , Porubsky Stefan , Gröne Hermann-Josef , Popovic Zoran V 
Published: January 2017

Abstract
γδ T cell subsets can be characterized, in part, by their secretion of select proinflammatory cytokines. The molecular mechanisms driving the diverse fates of γδ T cells have not been elucidated. We have previously shown that the attachment of myristic acid to the N-terminal glycine of proteins, termed N-myristoylation, is essential for αβ T cell development and activation. Here, we explore the potential role of this lipid modification on the activation of γδ T cells. In the absence of N-myristoylation, the CD27+ γδ T cell subset was dominantly affected. The cells produced high levels of IFN-γ upon stimulation. In addition, they were more sensitive to inhibition of the CaN-Nfat pathway than were γδ T cells with myristoylated CaN. N-Myristoylation was found to modulate activity of phosphatase CaN, a regulator of Nfat. In summary, the CaN–Nfat pathway regulates development and function of IFN-γ-producing γδ T cells, and its balanced activity is strongly dependent on CaN N-myristoylation.
Impact of Systemic Vascular Resistance on the Accuracy of the Pulsioflex Device
Authors: Biais Matthieu , Mazocky Elodie , Stecken Laurent , Pereira Bruno , Sesay Musa , Roullet Stéphanie , Quinart Alice , Sztark François 
Published: January 2017

BACKGROUND:
The accuracy of currently available devices using pulse contour analysis without external calibration for cardiac index (CI) estimation is negatively impacted by hyperdynamic states, low systemic vascular resistance (SVR), and abrupt changes in SVR. The aim of this study was to evaluate the accuracy of a new device, the Pulsioflex (Pulsion Medical System), in patients undergoing liver transplantation.

METHODS:
Thirty consecutive patients scheduled for liver transplantation were included. CI was monitored using pulmonary arterial catheter (CI-PAC) and Pulsioflex (CI-Pulsio). Simultaneous CI measurements were made intraoperatively at 9 different stages of the procedure.

RESULTS:
Two hundred seventy pairs of measurements were analyzed. The median CI-Pulsio values (3.3; interquartile range, 2.8–3.8 L·min−1·m2) were significantly different from the median CI-PAC (4.1; interquartile range, 3.1–5.0 L·min−1·m2; P < .0001). Bland and Altman analysis showed a mean bias of 0.8 L·min−1·m2 and 95% limit of agreement from −2.5 to 4.1 L·min−1·m2. Percentage error was 65% (95% confidence interval, 60%–71%). Considering the variations in CI between 2 stages, the comparison between changes in CI-PAC and changes in CI-Pulsio showed a mean bias of 0.1 L·min−1·m2 and 95% limit of agreement of −2.1 to 2.2 L·min−1·m2. When excluding changes in CI <0.5 L·min−1·m2 (154 paired analyzed), the concordance rate was 62% (95% confidence interval, 54%–70%). The bias between CI-PAC and CI-Pulsio was negatively correlated with SVR (r = −0.67, P < .0001). The bias between changes in CI-PAC and changes in CI-Pulsio was also negatively correlated with changes in SVR (r = −0.52, P < .0001).

CONCLUSIONS:
In patients undergoing liver transplantation, Pulsioflex does not accurately estimate CI. Its accuracy is highly impacted by SVR, and it is not able to track changes in CI when large variations in SVR occur.
Time- and Dose-Dependent Effects of Desflurane in Sensitized Airways
Authors: Zhou Jing , Iwasaki Sohshi , Yamakage Michiaki 
Published: January 2017

BACKGROUND:
Although the bronchodilatory actions of volatile anesthetics, such as halothane, isoflurane, and sevoflurane, have been well documented in previous studies, the properties of desflurane remain controversial. The aim of this study was to investigate the effects of desflurane at different concentrations and durations in an ovalbumin-sensitized guinea pig model of airway hyper-responsiveness.

METHODS:
Ovalbumin-sensitized animals (n = 176) were randomly assigned to 5 groups according to the minimum alveolar concentration (MAC) of desflurane they received: 0.0, 0.5, 1.0, 1.5, and 2.0 MAC. Total lung resistance in vivo, airway smooth muscle tension in vitro, and intracellular cyclic adenosine monophosphate (AMP) levels were measured to evaluate the effects of desflurane.

RESULTS:
In 5 sensitized groups, total lung resistance increased from baseline to peak at approximately 8 minutes and then decreased slowly until about 17 minutes with extended administration of desflurane. Desflurane dose-dependently increased total lung resistance with or without incremental doses of acetylcholine and reduced muscle tension with increasing concentrations of carbacholine. Cyclic AMP levels were increased by desflurane: at the 60-minute time point, cyclic AMP concentrations (means ± SD) with 0.5 MAC (1.96 ± 0.40) and 1.0 MAC (2.11 ± 0.50) desflurane were higher than those at the 8-minute time point (1.11 ± 0.23 and 1.32 ± 0.32).

CONCLUSIONS:
Desflurane exerted time- and dose-dependent effects and could be used at 0.5 and 1.0 MAC concentrations without significant bronchoconstriction in ovalbumin-sensitized guinea pigs. Cyclic AMP-mediated airway smooth muscle relaxation might be one mechanism by which desflurane induces bronchodilation.
A proteomic approach to identify endosomal cargoes controlling cancer invasiveness
Authors: Diaz-Vera Jesica , Palmer Sarah , Hernandez-Fernaud Juan Ramon , Dornier Emmanuel , Mitchell Louise E. , Macpherson Iain , Edwards Joanne , Zanivan Sara , Norman Jim C. 
Published: January 2017

ABSTRACT
We have previously shown that Rab17, a small GTPase associated with epithelial polarity, is specifically suppressed by ERK2 (also known as MAPK1) signalling to promote an invasive phenotype. However, the mechanisms through which Rab17 loss permits invasiveness, and the endosomal cargoes that are responsible for mediating this, are unknown. Using quantitative mass spectrometry-based proteomics, we have found that knockdown of Rab17 leads to a highly selective reduction in the cellular levels of a v-SNARE (Vamp8). Moreover, proteomics and immunofluorescence indicate that Vamp8 is associated with Rab17 at late endosomes. Reduced levels of Vamp8 promote transition between ductal carcinoma in situ (DCIS) and a more invasive phenotype. We developed an unbiased proteomic approach to elucidate the complement of receptors that redistributes between endosomes and the plasma membrane, and have pin-pointed neuropilin-2 (NRP2) as a key pro-invasive cargo of Rab17- and Vamp8-regulated trafficking. Indeed, reduced Rab17 or Vamp8 levels lead to increased mobilisation of NRP2-containing late endosomes and upregulated cell surface expression of NRP2. Finally, we show that NRP2 is required for the basement membrane disruption that accompanies the transition between DCIS and a more invasive phenotype.
Interplay between a cytosolic and a cell surface carbonic anhydrase in pH homeostasis and acid tolerance of Leishmania
Authors: Pal Dhiman Sankar , Abbasi Mazharul , Mondal Dipon Kumar , Varghese Binitha Anu , Paul Ritama , Singh Shalini , Datta Rupak 
Published: January 2017

ABSTRACT
Leishmania parasites have evolved to endure the acidic phagolysosomal environment within host macrophages. How Leishmania cells maintain near-neutral intracellular pH and proliferate in such a proton-rich mileu remains poorly understood. We report here that, in order to thrive in acidic conditions, Leishmania major relies on a cytosolic and a cell surface carbonic anhydrase, LmCA1 and LmCA2, respectively. Upon exposure to acidic medium, the intracellular pH of the LmCA1+/−, LmCA2+/− and LmCA1+/−:LmCA2+/− mutant strains dropped by varying extents that led to cell cycle delay, growth retardation and morphological abnormalities. Intracellular acidosis and growth defects of the mutant strains could be reverted by genetic complementation or supplementation with bicarbonate. When J774A.1 macrophages were infected with the mutant strains, they exhibited much lower intracellular parasite burdens than their wild-type counterparts. However, these differences in intracellular parasite burden between the wild-type and mutant strains were abrogated if, before infection, the macrophages were treated with chloroquine to alkalize their phagolysosomes. Taken together, our results demonstrate that haploinsufficiency of LmCA1 and/or LmCA2 renders the parasite acid-susceptible, thereby unravelling a carbonic anhydrase-mediated pH homeostatic circuit in Leishmania cells.
Molecular identification of endophytic fungi isolated from the tuber of Dahlia variabilis and exploration of their ability in producing ÃŸ-galactosidase
Authors: SARYONO SARYONO , RAKHMANA SENJAVI , RAHAYU FITRI , ARDHI AULIA , RUSLI RUSLI , PRATIWI NOVA WAHYU , NUGROHO TITANIA T. 
Published: January 2017

Abstract. Saryono, Rakhmana S, Rahayu F, Ardhi A, Rusli, Pratiwi NW, Nugroho TT. 2017. Molecular identification of endophytic fungi isolated from the tuber of Dahlia variabilis and exploration of their ability in producing ÃŸ-galactosidase. Biodiversitas 18: 145-152.LBKURCC67 and LBKURCC69 were endophytic fungi isolated from the tubers of dahlia (Dahlia variabilis) in Padang Panjang, West Sumatra. This study aimed to identify the microscopic and molecular characteristics of endophytic fungi LBKURCC67 and LBKURCC69 based on ITS-rDNA region sequences and determine the ability of the isolates to produce Î²-galactosidase. Microscopic characteristic of LBKURCC67 and LBKURCC69 indicated that both isolates belonged to Fusarium sp. Furthermore, DNA sequence analysis based on mega BLAST program showed that LBKURCC67 was identified to be Fusarium solani with 99% homology and LBKURCC69 showed the perfect homology similarity with Fusarium oxysporum. Meanwhile, Î²-galactosidase activity was determined based on the amount of o-nitrophenol produced from the substrate o-nitrophenil-Î²-D-galactopiranoside, whilst the specific activity was indicated by activity per unit of proteins. The result showed that both fungi could produce Î²-galactosidase. However, the enzyme activity did not increase significantly at various production times of 2, 4, 6, 8, and 10 days. The highest enzyme activity of LBKURCC67 and LBKURCC69 were 0.163 Â± 0.064 U/mL (at day 8) and 0.126 Â± 0.039 U/mL (at day 2) respectively. LBKURCC69 showed higher specific Î²-galactosidase activity (1.886 Â± 0.277 U/mg) than LBKURCC67 (1.179 Â± 0.081 U/mg).
Keywords: ÃŸ-galactosidase, dahlia tuber, endophytic fungi, Fusarium, ITS-rDNAÂ
Karangwangi peopleâ€™s local knowledge of bamboo and its role: Implications for management of cultural keystone species
Authors: PARTASASMITA RUHYAT , ANâ€™AMILLAH AZIFAH , ISKANDAR JOHAN , MUTAQIN ASEP ZAINAL , ANNISA ANNISA , RATNINGSIH NINING 
Published: January 2017

Abstract. Partasasmita R, Anâ€™amillah A, Iskandar J, Mutaqin AZ, Annisa. 2017. Traditional knowledge of bamboo and its role in Karangwangi Village Cianjur, West Java: Implications for management of cultural keystone species. Biodiversitas 18: 275-282. Indonesia has a high diversity of bamboo, with more than 160 species can be found around the country. Nowadays, this plant is rarely planted around rural communities of West Java due to land use conversions and economic reasons. This research is to discuss traditional knowledge of local people of Karangwangi Village, Cianjur, West Java, Indonesia on diversity of bamboo and its role. The qualitative method was used in this study, while the data collection techniques, namely semi structure interview or deep interview and observation participant are applied. The result of study shows that based on the traditional knowledge of Karangwangi people, it has been reviled 13 species of bamboo, e.g., Bambusa blumeana J.A. & J.H. Schultz, Bambusa glaucophylla Widjaja, Bambusa maculata Widjaja, Bambusa vulgaris Schrad. Ex. Wndl. var vulgaris, Bambusa vulgaris Schrad. Ex. Wndl. var. striata, Gigantochloa apus J.A. & J.H. Schultz. Kurz., Gigantochloa atrovilacea Widjaja, Gigantochloa atter (Hassk.) Kurz., Gigantochloa hasskarliana (Kurz.) Backer ex Heyne, Gigantochloa pseudoarundinacea (Steud.) Widjaja, Schizostachyum iraten Widjaja, Schizostachyum sp., and Dinochloa scandens (Blume ex Neese) Kuntze. On the basis of deep interview, these bamboo species have traditionally various used, such as building materials, furnitures, household appliances, working tools, medicine, consumption, traditional musical instruments, traditional game, and decoration. In addition, Bamboo soften feature prominently in the language, ceremonies, and narratives of native peoples, so it can be considered as a cultural icons. The implications of these discussion are with respect to conservation as cultural keystone species.
Keywords: Bamboo, biodiversity, cultural keystone species, ethnobiology, Karangwangi
DISTRIBUIÇÃO ESPACIAL E TEMPORAL DE UMA COMUNIDADE DE ANFÍBIOS ANUROS DO MUNICÍPIO DE RIO CLARO, SÃO PAULO, BRASIL
Authors: Toledo Luis Felipe , Zina Juliana , Haddad Celio Fernando Baptista 
Published: January 2017

A compreensão da composição e inter-relações das comunidades faunísticas são fundamentais para a execução de medidas conservacionistas em ambientes florestais.
No presente estudo nos avaliamos a comunidade de anfíbios de uma floresta estadual no município de Rio Claro, SP, com enfoque na biologia reprodutiva. Foram registradas 21
espécies, de quarto famílias: Bufonidae, Hylidae, Leptodactylidae e Microhylidae. As espécies da família Bufonidae reproduziram durante a estação fria e seca do ano e as demais somente durante a estação quente e chuvosa. Além de segregação temporal, algumas espécies dividiram o ambiente físico durante o período reprodutivo. Acentuada
sobreposição temporal e espacial foi observada entre espécies filogeneticamente próximas, como entre Hyla nana e H. sanborni, entre Scinax fuscovarius e Scinax sp.
(aff. similis), e entre Leptodactylus fuscus, L. mystacinus, e Leptodactylus sp. (gr. fuscus). Nestes casos, diferenças acústicas podem ter sido o principal fator responsável pela
segregação reprodutiva. Ausência de segregação reprodutiva foi observada entre espécies do gênero Bufo, as quais foram vistas em amplexo interespecífico, apesar das diferenças
acústicas entre seus cantos de anúncio.
Parallel diurnal fluctuation of testosterone, androstenedione, dehydroepiandrosterone and 17OHprogesterone as assessed in serum and saliva: validation of a novel liquid chromatography-tandem mass spectrometry method for salivary steroid profiling
Authors: Mezzullo Marco , Fazzini Alessia , Gambineri Alessandra , Di Dalmazi Guido , Mazza Roberta , Pelusi Carla , Vicennati Valentina , Pasquali Renato , Pagotto Uberto , Fanelli Flaminia 
Published: January 2017

AbstractBackground:Salivary androgen testing represents a valuable source of biological information. However, the proper measurement of such low levels is challenging for direct immunoassays, lacking adequate accuracy. In the last few years, many conflicting findings reporting low correlation with the serum counterparts have hampered the clinical application of salivary androgen testing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) makes it possible to overcome previous analytical limits, providing new insights in endocrinology practice.Methods:Salivary testosterone (T), androstenedione (A), dehydroepiandrosterone (DHEA) and 17OHprogesterone (17OHP) were extracted from 500µL of saliva, separated in 9.5 min LC-gradient and detected by positive electrospray ionization – multiple reaction monitoring. The diurnal variation of salivary and serum androgens was described by a four paired collection protocol (8 am, 12 am, 4 pm and 8 pm) in 19 healthy subjects.Results:The assay allowed the quantitation of T, A, DHEA and 17OHP down to 3.40, 6.81, 271.0 and 23.7 pmol/L, respectively, with accuracy between 83.0 and 106.1% for all analytes. A parallel diurnal rhythm in saliva and serum was observed for all androgens, with values decreasing from the morning to the evening time points. Salivary androgen levels revealed a high linear correlation with serum counterparts in both sexes (T: R>0.85; A: R>0.90; DHEA: R>0.73 and 17OHP: R>0.89; p<0.0001 for all).Conclusions:Our LC-MS/MS method allowed a sensitive evaluation of androgen salivary levels and represents an optimal technique to explore the relevance of a comprehensive androgen profile as measured in saliva for the study of androgen secretion modulation and activity in physiologic and pathologic states.
Simplified Iterated Lavrentiev Regularization for Nonlinear Ill-Posed Monotone Operator Equations
Authors: Mahale Pallavi 
Published: January 2017

Abstract
Mahale and Nair [12] considered an iterated
form of Lavrentiev regularization
for obtaining stable approximate solutions for
ill-posed nonlinear equations of the form

F
⁢

(
x
)

=
y

${F(x)=y}$

, where

F
:

D
⁢

(
F
)

⊆
X
→
X

${F:D(F)\subseteq X\to X}$

is a nonlinear monotone operator and X is a Hilbert space. They considered an a posteriori strategy to find a stopping index which not only led to the convergence of the method, but also gave an order optimal error estimate
under a general source condition. However, the iterations defined
in [12] require calculation of Fréchet derivatives at each iteration. In this paper,
we consider a simplified version of the iterated Lavrentiev regularization which will involve calculation of the Fréchet derivative only at the point

x
0

${x_{0}}$

, i.e., at the initial approximation of the exact solution

x
†

${x^{\dagger}}$

. Moreover, the general source condition and stopping rule which we use in this paper involve calculation of the Fréchet derivative at the point

x
0

${x_{0}}$

,
instead at the unknown exact solution

x
†

${x^{\dagger}}$

as in [12].
Semantically-based functions of noun-class markers in Tagbana
Authors: Kiyofon Antoine , Duffley Patrick 
Published: January 2017

Abstract
This paper addresses the use of noun-class markers in Tagbana from the perspective of a cognitively-inspired approach based on Langacker’s (2000. Grammar and conceptualization. Berlin & New York: Mouton de Gruyter) semiological principle. Drawing on this basic tenet of Cognitive Grammar according to which the symbolic function of language consists in making speakers’ conceptualizations auditorily or visually perceptible, it demonstrates that in syntactic constructions composed of ‘noun-stem+noun-class marker’ and ‘noun-class marker+identifier’, noun-class markers fulfil the semantic function of making explicit the way the speaker conceives of the experiential entity referred to in the utterance.This view goes beyond form-centred functions such as referent-tracking to include the signifying of complex conceptualizations involving more than one noun-class marker with the same noun-stem, as well as the discourse functions of indicating topicality, insistence on a referent’s existence and contrastive focus.
Strict stability with respect to initial time difference for Caputo fractional differential equations by Lyapunov functions
Authors: Agarwal Ravi P. , O’Regan Donal , Hristova Snezhana 
Published: January 2017

Abstract
The strict stability properties are generalized to nonlinear Caputo fractional differential equations in the case when both initial points and initial times are changeable.
Using Lyapunov functions, some criteria for strict stability, eventually strict stability and strict practical stability are obtained.
A brief overview of different types of derivatives in the literature related to the application of Lyapunov functions to Caputo fractional equations are given, and their advantages and disadvantages are discussed with several examples.
The Caputo fractional Dini derivative with respect to to initial time difference is used to obtain some sufficient conditions.