bluuebunny/crossref_metadata_2025_split huggingface dataset:
IRF6 Regulates the Expression of IL-36γ by Human Oral Epithelial Cells in Response to Porphyromonas gingivalis
Authors: Huynh Jennifer , Scholz Glen M. , Aw Jiamin , Kwa Mei Qi , Achuthan Adrian , Hamilton John A. , Reynolds Eric C. 
Published: January 2016

Abstract
IFN regulatory factors (IRFs) help to shape the immune response to pathogens by imparting signaling specificity to individual TLRs. We recently demonstrated that IRF6 provides specificity to TLR2 signaling in oral epithelial cells. TLR2 plays an important role in eliciting inflammation to Porphyromonas gingivalis, a keystone pathogen in periodontitis. Therefore, we investigated a role for IRF6 in mediating the inflammatory cytokine response of oral epithelial cells to P. gingivalis. IRF6 expression was strongly upregulated when human oral epithelial cells were challenged with P. gingivalis. Moreover, gene silencing and gene promoter experiments indicated that IRF6 acts downstream of IL-1R–associated kinase 1 to stimulate the expression of the IL-1 family cytokine IL-36γ in response to P. gingivalis. IRF6 and IL-1R–associated kinase 1 also regulated the stimulation of IL-36γ expression by a TLR2 agonist. IL-36γ was shown to elicit inflammatory responses by human monocyte-derived dendritic cells and macrophages, including the expression of the neutrophil chemokines IL-8 and CXCL1, as well as the Th17 chemokine CCL20. IL-36γ similarly stimulated their expression by human oral epithelial cells. Significantly, the Th17 cytokine IL-17 not only stimulated the expression of important regulators of neutrophil recruitment and survival by oral epithelial cells, but IL-17 also stimulated them to express IL-36γ. Thus, our findings suggest that IRF6 is likely to promote inflammation to P. gingivalis through its regulation of IL-36γ.
Aminobisphosphonates Synergize with Human Cytomegalovirus To Activate the Antiviral Activity of Vγ9Vδ2 Cells
Authors: Daguzan Charline , Moulin Morgane , Kulyk-Barbier Hanna , Davrinche Christian , Peyrottes Suzanne , Champagne Eric 
Published: January 2016

Abstract
Human Vγ9Vδ2 T cells are activated through their TCR by neighboring cells producing phosphoantigens. Zoledronate (ZOL) treatment induces intracellular accumulation of the phosphoantigens isopentenyl pyrophosphate and ApppI. Few attempts have been made to use immunomanipulation of Vγ9Vδ2 lymphocytes in chronic viral infections. Although Vγ9Vδ2 T cells seem to ignore human CMV (HCMV)–infected cells, we examined whether they can sense HCMV when a TCR stimulus is provided with ZOL. Fibroblasts treated with ZOL activate Vγ9Vδ2 T cells to produce IFN-γ but not TNF. Following the same treatment, HCMV-infected fibroblasts stimulate TNF secretion and an increased production of IFN-γ, indicating that Vγ9Vδ2 cells can sense HCMV infection. Increased lymphokine production was observed with most clinical isolates and laboratory HCMV strains, HCMV-permissive astrocytoma, or dendritic cells, as well as “naive” and activated Vγ9Vδ2 cells. Quantification of intracellular isopentenyl pyrophosphate/ApppI following ZOL treatment showed that HCMV infection boosts their accumulation. This was explained by an increased capture of ZOL and by upregulation of HMG-CoA synthase and reductase transcription. Using an experimental setting where infected fibroblasts were cocultured with γδ cells in submicromolar concentrations of ZOL, we show that Vγ9Vδ2 cells suppressed substantially the release of infectious particles while preserving uninfected cells. Vγ9Vδ2 cytotoxicity was decreased by HCMV infection of targets whereas anti–IFN-γ and anti-TNF Abs significantly blocked the antiviral effect. Our experiments indicate that cytokines produced by Vγ9Vδ2 T cells have an antiviral potential in HCMV infection. This should lead to in vivo studies to explore the possible antiviral effect of immunostimulation with ZOL in this context.
Attenuation of extubation responses: Comparison of prior treatment with verapamil and dexmedetomidine
Authors: Mistry Tuhin , Purohit Shobha , Arora Gunjan , Gill Nitesh , Sharma Jaya 
Published: January 2016

Abstract
Background: Tracheal extubation is almost always associated with stress response, airway response, and arrhythmias. There are many ways to attenuate this stress response. We have compared verapamil and dexmedetomidine on attenuation of these responses. Materials and Methods: Thirty patients (American Society of Anaesthesiologists Grade I, II) scheduled for spinal surgeries under general anaesthesia were randomly divided into two groups. At the end of surgery, after return of spontaneous efforts (bispectral index >80), in “Group V” verapamil 0.1 mg/kg and in “Group D” dexmedetomidine 0.3 mcg/kg were administered as bolus intravenously over one minute. Heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) were recorded just before (T0) and 2 min after intravenous administration of study medications (TM), just after oral suction (TS), immediately after extubation (TE) and 1, 3, 5 and 10 min postextubation (E1, E3, E5, E10). Duration of emergence and extubation, quality of extubation, Richmond Agitation Sedation Scale (RASS) score and time to reach modified Aldrete score ≥9 were compared. Results: HR, SBP, DBP, MAP were higher in Group V than Group D, but statistically insignificant (P > 0.05). Extubation quality scores was 1 for 20%, 2 for 60% and 3 for 20% patients in Group V, whereas 1 in 80%, 2 in 20% in Group D. There was occurrence of bradycardia within 2 min of administration of drug in one patient in Group D. RASS score was in the range of -1 to +1 in >90% patient in Group V, whereas -3 to -1 in 80% cases in Group D. Conclusion: Single dose of dexmedetomidine (0.3 mcg/kg) given before extubation produced significant attenuation of circulatory and airway responses during extubation as compared to verapamil (0.1 mg/kg).
Neurosurgery in morbidly obese patients
Authors: Mohanaselvi S. , Subramanian Rajkumar , Sardar Arijit , Anand Rahul , Agarwal Anil , Khanna Puneet 
Published: January 2016

AbstractObesity has significant impact on various organ systems of the body and thus needs a well-planned anaesthetic management. Obese patients with multiple co morbidities are expected to have more complications than normal individuals. Obesity may influence the risk of aneurysm formation and rupture and/or the outcome of patients who have aneurysmal SAH. Most of the neurosurgeries require different patient positions for adequate exposure of surgical site. Moreover morbidly obese patient means a huge and heavy patient who will require bigger operating table and other accessories and their implications. Confusion regarding the risks and benefits of mechanical and pharmacological prophylaxis in neurosurgical patients for DVT with risks of major and minor haemorrhage still persists. The anesthetic concerns in an obese patient undergoing neurosurgery have not been studied so far. This review aims in discussing obesity in neurosurgical patients.
Clinical review of non-invasive intracranial pressure measurement in medical cases
Authors: Nair Shalini 
Published: January 2016

AbstractIntracranial pressure (ICP) measurement plays a vital role in decision making in neurological emergencies. Invasive methods of ICP monitoring have been the gold standard. Advent of various non-invasive techniques has widened the option of ICP measurement in medical cases as well. We illustrate two cases where optic nerve sheath measurements helped in managing raised ICP and time the need for surgical intervention and review the literature on non-invasive ICP measurement.
Difficult airway leading to carbon dioxide narcosis in a case of fixed cervical spine
Authors: Yadav Rahul , Pandia Mihir , Bithal Parmod , Bharati Sachidanand , Kapoor Indu 
Published: January 2016

AbstractInability to secure the airway of a patient after induction of anaesthesia may lead to serious consequences including permanent brain damage and even death. Hypoxia is quite common in difficult intubations especially when it is difficult to ventilate the patient. However, carbon dioxide retention severe enough to cause carbon dioxide narcosis and delayed recovery is a rare occurrence. Here, we report a case of a craniovertebral junction anomaly where inadequate ventilation after induction of anaesthesia resulted in carbon dioxide narcosis and delayed awakening. A 54-year-old, American Society of Anesthesiologists II female patient weighing 70 kg with a diagnosis of craniovertebral junction was scheduled for implant removal for dislodged occipital screw. Fibreoptic intubation was attempted after induction of anaesthesia and muscle paralysis. Even after multiple attempts, intubation could not be done and ventilation by face mask became difficult. Though oxygen saturation could be maintained with the insertion of a laryngeal mask airway (LMA), ventilation was not adequate. The patient remained unresponsive long after discontinuation of anaesthetic agent and reversal of muscle paralysis. Subsequent blood gas analysis showed severe carbon dioxide retention and respiratory acidosis. Patient was given assist control mechanical ventilation through LMA. LMA was removed after improvement in sensorium and the blood gas picture.
A Scoping Review of Clinical Practice Improvement Methodology Use in Rehabilitation
Authors: Lamontagne Marie-Eve , Gagnon Cynthia , Allaire Anne-Sophie , Noreau Luc 
Published: January 2016

Context The Clinical Practice Improvement (CPI) approach is a methodological and quality improvement approach that has emerged and is gaining in popularity. However, there is no systematic description of its use or the determinants of its practice in rehabilitation settings. Method We performed a scoping review of the use of CPI methodology in rehabilitation settings. Results A total of 103 articles were reviewed. We found evidence of 13 initiatives involving CPI with six different populations. A total of 335 citations of determinants were found, with 68.7% related to CPI itself. Little information was found about what type of external and internal environment, individual characteristics and implementation process might facilitate or hinder the use of CPI. Conclusion Given the growing popularity of this methodological approach, CPI initiatives would gain from increasing knowledge of the determinants of its success and incorporating them in future implementation.
Retrieval of sodium number density profiles in the mesosphere and lower thermosphere from SCIAMACHY limb emission measurements
Authors: Langowski M. P. , von Savigny C. , Burrows J. P. , Rozanov V. V. , Dunker T. , Hoppe U.-P. , Sinnhuber M. , Aikin A. C. 
Published: January 2016

Abstract. An algorithm has been developed for the retrieval of sodium atom (Na) number density on a latitude and altitude grid from SCIAMACHY (SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY) limb measurements of the Na resonance fluorescence. The results are obtained between 50 and 150 km altitude and the resulting global seasonal variations of Na are analyzed. The retrieval approach is adapted from that used for the retrieval of magnesium atom (Mg) and magnesium ion (Mg+) number density profiles recently reported by Langowski et al. (2014). Monthly mean values of Na are presented as a function of altitude and latitude. This data set was retrieved from the 4 years of spectroscopic limb data of the SCIAMACHY mesosphere and lower thermosphere (MLT) measurement mode (mid-2008 to early 2012). The Na layer has a nearly constant peak altitude of 90–93 km for all latitudes and seasons, and has a full width at half maximum of 5–15 km. Small but significant seasonal variations in Na are identified for latitudes less than 40°, where the maximum Na number densities are 3000–4000 atoms cm−3. At middle to high latitudes a clear seasonal variation with a winter maximum of up to 6000 atoms cm−3 is observed. The high latitudes, which are only measured in the summer hemisphere, have lower number densities, with peak densities being approximately 1000 Na atoms cm−3. The full width at half maximum of the peak varies strongly at high latitudes and is 5 km near the polar summer mesopause, while it exceeds 10 km at lower latitudes. In summer the Na atom concentration at high latitudes and at altitudes below 88 km is significantly smaller than that at middle latitudes. The results are compared with other observations and models and there is overall a good agreement with these.
Comparing the Effect of One-way versus Two-way Tasks on Iranian Intermediate EFL Learners’ Phrasal Verb Learning
Authors: Khodareza Mohammad Reza , Shabani Gholamhossein 
Published: January 2016

<p>The aim of this study was to investigate the effect of one-way and two-way tasks, as two various techniques for teaching vocabulary, on Iranian intermediate EFL learners' phrasal verb learning. The participants in this study were sixty EFL learners majoring in TEFL, all of whom were in the second semester of academic year 2015. In order to establish a homogenized group, a test of proficiency titled CELT was administered to ninety sophomore students, and sixty were selected to serve the purpose of the study. They were intermediate students with the age range within 18 to 29. After the administration of the proficiency test as the homogeneity, they were then divided into two equal comparison groups, either of whom comprised thirty participants: one-way task (OWT) and two-way task (TWT) groups. A pretest, comprising forty multiple-choice phrasal verbs, was administered to the both groups. Then both groups underwent twelve sessions of treatment (treatment for the TWT group, and placebo for the OWT group). The OWT group was taught phrasal verbs by means of one-way task, and the TWT group was taught vocabulary via two-way tasks as input. After the treatment period, the same version of phrasal verb test was administered to both groups as posttest to examine the effectiveness of the treatment. Both groups in this study were taught by the same researcher and through the same methodology. The data were analyzed through running paired-samples and independent samples t-tests, the outcome of which revealed that both the OWT group and the TWT group had vocabulary gains but the effect of two-way tasks on phrasal verbs learning was more salient than that of the one-way task group. The implications and recommendations were also presented.</p>
Aviation Risk Management to Comparative Performance in Air China with American Airlines
Authors: Alnuaimi Qussay A. B. , Xia Xinping , Jawad Firas Aziz M. 
Published: January 2016

<p>This paper presents a model comparison between the Air China and American Airline. The aim of this study is to research on strong and weak points of these companies on the grounds. Both the countries are from in the continent of Asia and North America and the world. It also concentrates on the domestic and international aviation sector to comparative both Air China and American Airline with political and economic volatility. The sample of SWOT is used to test indexes and to measure volume of strengths and weakness to both the firms. The model represents one of the tools of Aviation Risk Management to evaluate Air China through find all the elements of development and address the weaknesses and shortcomings of the Chinese aviation company in order to compete with the major international airlines.<strong></strong></p>
Vrijheid van godsdienst en de grens tussen radicale en strafbare uitlatingen
Authors: Nieuwenhuis Aernout 
Published: January 2016

Freedom of religion and the dividing line between the merely provocative and the definitely criminal public statement.
Certain radical public acts of expression, those which for instance incite hatred or recruit participants for armed conflict, are punishable under criminal law, even when these pronouncements are religiously motivated. This study examines which statements incur punishment, and the role freedom of religion and freedom of expression play when making this assessment.
Official Methods for the Determination of Minerals and Trace Elements in Infant Formula and Milk Products: A Review
Authors: Poitevin Eric 
Published: January 2016

Abstract
The minerals and trace elements that account for about 4% of total human body mass serve as materials and regulators in numerous biological activities in body structure building. Infant formula and milk products are important sources of endogenic and added minerals and trace elements and hence, must comply with regulatory as well as nutritional and safety requirements. In addition, reliable analytical data are necessary to support product content and innovation, health claims, or declaration and specific safety issues. Adequate analytical platforms and methods must be implemented to demonstrate both the compliance and safety assessment of all declared and regulated minerals and trace elements, especially trace-element contaminant surveillance. The first part of this paper presents general information on the mineral composition of infant formula and milk products and their regulatory status. In the second part, a survey describes the main techniques and related current official methods determining minerals and trace elements in infant formula and milk products applied for by various international organizations (AOAC INTERNATIONAL, the International Organization for Standardization, the International Dairy Federation, and the European Committe for Standardization). The third part summarizes method officialization activities by Stakeholder Panels on Infant Formula and Adult Nutritionals and Stakeholder Panel on Strategic Food Analytical Methods. The final part covers a general discussion focusing on analytical gaps and future trends in inorganic analysis that have been applied for in infant formula and milk-based products.
New Methods for the Analysis of Fat-Soluble Vitamins in Infant Formula and Adult Nutritionals
Authors: Gilliland Don 
Published: January 2016

Abstract
The AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) defined fat-soluble vitamins (FSVs) to include vitamins A, D, E, and K. The levels of FSVs have been closely scrutinized because of a number of factors, including nutrition value especially in foods used to provide sole-source nutrition and the potential for health risks associated with content both below and above recommended levels. Stricter scrutiny and emphasis on nutrient-level compliance with regulatory requirements placed an increased demand on analytical methods that were fit-for-purpose, provided accurate and precise results. Over time, compendial methods have been developed and published by organizations such as AOAC INTERNATIONAL, the European Committee for Standardization, the International Dairy Federation, and the International Organization for Standardization, among others. In general, these methods have shown adequate precision for regulatory compliance based on example food matrixes for which they were designed. However, method evaluation for infant formulas and adult nutritional products was limited to very few matrixes within these categories. As such, method applicability gaps were noted and correlated with more complicated or diverse product matrixes. AOAC undertook the task of modernizing test methods for the determination of nutrients in infant formulas and in adult nutritional products formulated specifically for both healthy adults and those requiring specific nutritional intake. Composition of products in this category continued to evolve, which in turn presented increasing and new challenges to analytical methodology. A summary of a new generation of candidate compendial methods for determination of FSVs in these matrixes, identified by SPIFAN, is presented here.
Functional Differences Between Placental Micro- and Macrovascular Endothelial Colony-Forming Cells
Authors: Solomon Ioana , O'Reilly Megan , Ionescu Lavinia , Alphonse Rajesh S. , Rajabali Saima , Zhong Shumei , Vadivel Arul , Shelley W. Chris , Yoder Mervin C. , Thébaud Bernard 
Published: January 2016

Abstract

Alterations in the development of the placental vasculature can lead to pregnancy complications, such as preeclampsia. Currently, the cause of preeclampsia is unknown, and there are no specific prevention or treatment strategies. Further insight into the placental vasculature may aid in identifying causal factors. Endothelial colony-forming cells (ECFCs) are a subset of endothelial progenitor cells capable of self-renewal and de novo vessel formation in vitro. We hypothesized that ECFCs exist in the micro- and macrovasculature of the normal, term human placenta. Human placentas were collected from term pregnancies delivered by cesarean section (n = 16). Placental micro- and macrovasculature was collected from the maternal and fetal side of the placenta, respectively, and ECFCs were isolated and characterized. ECFCs were CD31+, CD105+, CD144+, CD146+, CD14−, and CD45−, took up 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate-labeled acetylated low-density lipoprotein, and bound Ulex europaeus agglutinin 1. In vitro, macrovascular ECFCs had a greater potential to generate high-proliferative colonies and formed more complex capillary-like networks on Matrigel compared with microvascular ECFCs. In contrast, in vivo assessment demonstrated that microvascular ECFCs had a greater potential to form vessels. Macrovascular ECFCs were of fetal origin, whereas microvascular ECFCs were of maternal origin. ECFCs exist in the micro- and macrovasculature of the normal, term human placenta. Although macrovascular ECFCs demonstrated greater vessel and colony-forming potency in vitro, this did not translate in vivo, where microvascular ECFCs exhibited a greater vessel-forming ability. These important findings contribute to the current understanding of normal placental vascular development and may aid in identifying factors involved in preeclampsia and other pregnancy complications.

Significance
This research confirms that resident endothelial colony-forming cells (ECFCs) exist in the micro- and macrovasculature of the normal, term human placenta. Their isolation from two different anatomical locations yields two functionally different ECFC populations. Investigation of these ECFC populations during placental pathologies, such as preeclampsia, may lead to a better understanding of the disease process and aid in developing new therapies.
Effect of Induced Pluripotent Stem Cell Technology in Blood Banking
Authors: Focosi Daniele , Pistello Mauro 
Published: January 2016

SummaryPopulation aging has imposed cost-effective alternatives to blood donations. Artificial blood is still at the preliminary stages of development, and the need for viable cells seems unsurmountable. Because large numbers of viable cells must be promptly available for clinical use, stem cell technologies, expansion, and banking represent ideal tools to ensure a regular supply. Provided key donors can be identified, induced pluripotent stem cell (iPSC) technology could pave the way to a new era in transfusion medicine, just as it is already doing in many other fields of medicine. The present review summarizes the current state of research on iPSC technology in the field of blood banking, highlighting hurdles, and promises.SignificanceThe aging population in Western countries is causing a progressive reduction of blood donors and a constant increase of blood recipients. Because blood is the main therapeutic option to treat acute hemorrhage, cost-effective alternatives to blood donations are being actively investigated. The enormous replication capability of induced pluripotent stem cells and their promising results in many other fields of medicine could be an apt solution to produce the large numbers of viable cells required in transfusion and usher in a new era in transfusion medicine. The present report describes the potentiality, technological hurdles, and promises of induced pluripotent stem cells to generate red blood cells by redifferentiation.