bluuebunny/crossref_metadata_2025_split huggingface dataset:
sTRAIL-iRGD is a promising therapeutic agent for gastric cancer treatment
Authors: Huang Ying , Li Xihan , Sha Huizi , Zhang Lianru , Bian Xinyu , Han Xiao , Liu Baorui 
Published: March 2017

AbstractTumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively kills tumor cells and augments chemotherapeutics in vivo. Here, we developed sTRAIL-iRGD, a recombinant protein consisting of sTRAIL fused to CRGDKGPDC, a C-terminal end binding peptide with an integrin-binding arginine-glycine-aspartic acid (iRGD) motif. CRGDKGPDC is a tumor-homing peptide with high penetration into tumor tissue and cells. We found that sTRAIL-iRGD internalized into cultured gastric cancer tumor cells and localized to both the tumor mass in vivo and three-dimensional multicellular spheroids in vitro. sTRAIL-iRGD had an antitumor effect in tumor cell lines, multicellular spheroids and nude mice with tumors. Repeated treatment with sTRAIL-iRGD reduced tumor growth and volume in vivo. Mice treated with sTRAIL-iRGD and paclitaxel (PTX) in combination showed no sign of sTRAIL-iRGD-related liver toxicity. Our data suggest that sTRAIL-iRGD is a promising anti-gastric cancer agent with high selectivity and limited systemic toxicity.
The signs of Antarctic ozone hole recovery
Authors: Kuttippurath Jayanarayanan , Nair Prijitha J. 
Published: March 2017

AbstractAbsorption of solar radiation by stratospheric ozone affects atmospheric dynamics and chemistry, and sustains life on Earth by preventing harmful radiation from reaching the surface. Significant ozone losses due to increases in the abundances of ozone depleting substances (ODSs) were first observed in Antarctica in the 1980s. Losses deepened in following years but became nearly flat by around 2000, reflecting changes in global ODS emissions. Here we show robust evidence that Antarctic ozone has started to recover in both spring and summer, with a recovery signal identified in springtime ozone profile and total column measurements at 99% confidence for the first time. Continuing recovery is expected to impact the future climate of that region. Our results demonstrate that the Montreal Protocol has indeed begun to save the Antarctic ozone layer.
Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro
Authors: Meininger Susanne , Blum Carina , Schamel Martha , Barralet Jake E. , Ignatius Anita , Gbureck Uwe 
Published: March 2017

AbstractDicalcium phosphate cement preparation requires the addition of setting retarders to meet clinical requirements regarding handling time and processability. Previous studies have focused on the influence of different setting modifiers on material properties such as mechanical performance or injectability, while ignoring their influence on biological cement properties as they are used in low concentrations in the cement pastes and the occurrence of most compounds in human tissues. Here, analyses of both material and biological behavior were carried out on samples with common setting retardants (citric acid, sodium pyrophosphate, sulfuric acid) and novel (phytic acid). Cytocompatibility was evaluated by in vitro tests with osteoblastic (hFOB 1.19) and osteoclastic (RAW 264.7) cells. We found cytocompatibility was better for sodium pyrophosphate and phytic acid with a three-fold cell metabolic activity by WST-1 test, whereas samples set with citric acid showed reduced cell number as well as cell activity. The compressive strength (CS) of cements formed with phytic acid (CS = 13 MPa) were nearly equal to those formed with citric acid (CS = 15 MPa) and approximately threefold higher than for other setting retardants. Due to a proven cytocompatibility and high mechanical strength, phytic acid seems to be a candidate replacement setting retardant for dicalcium phosphate cements.
Amine-Functionalized Covalent Organic Framework for Efficient SO2 Capture with High Reversibility
Authors: Lee Gang-Young , Lee Joohyeon , Vo Huyen Thanh , Kim Sangwon , Lee Hyunjoo , Park Taiho 
Published: March 2017

AbstractRemoving sulfur dioxide (SO2) from exhaust flue gases of fossil fuel power plants is an important issue given the toxicity of SO2 and subsequent environmental problems. To address this issue, we successfully developed a new series of imide-linked covalent organic frameworks (COFs) that have high mesoporosity with large surface areas to support gas flowing through channels; furthermore, we incorporated 4-[(dimethylamino)methyl]aniline (DMMA) as the modulator to the imide-linked COF. We observed that the functionalized COFs serving as SO2 adsorbents exhibit outstanding molar SO2 sorption capacity, i.e., PI-COF-m10 record 6.30 mmol SO2 g−1 (40 wt%). To our knowledge, it is firstly reported COF as SO2 sorbent to date. We also observed that the adsorbed SO2 is completely desorbed in a short time period with remarkable reversibility. These results suggest that channel-wall functional engineering could be a facile and powerful strategy for developing mesoporous COFs for high-performance reproducible gas storage and separation.
Demographic associations for autoantibodies in disease-free individuals of a European population
Authors: Haller-Kikkatalo Kadri , Alnek Kristi , Metspalu Andres , Mihailov Evelin , Metsküla Kaja , Kisand Kalle , Pisarev Heti , Salumets Andres , Uibo Raivo 
Published: March 2017

AbstractThe presence of autoantibodies usually precedes autoimmune disease, but is sometimes considered an incidental finding with no clinical relevance. The prevalence of immune-mediated diseases was studied in a group of individuals from the Estonian Genome Project (n = 51,862), and 6 clinically significant autoantibodies were detected in a subgroup of 994 (auto)immune-mediated disease-free individuals. The overall prevalence of individuals with immune-mediated diseases in the primary cohort was 30.1%. Similarly, 23.6% of the participants in the disease-free subgroup were seropositive for at least one autoantibody. Several phenotypic parameters were associated with autoantibodies. The results suggest that (i) immune-mediated diseases are diagnosed in nearly one-third of a random European population, (ii) 6 common autoantibodies are detectable in almost one-third of individuals without diagnosed autoimmune diseases, (iii) tissue non-specific autoantibodies, especially at high levels, may reflect preclinical disease in symptom-free individuals, and (iv) the incidental positivity of anti-TPO in men with positive familial anamnesis of maternal autoimmune disease deserves further medical attention. These results encourage physicians to evaluate autoantibodies in addition to treating a variety of patient health complaints to detect autoimmune-mediated disease early.
Antiproliferative effects of ruthenium-based nucleolipidic nanoaggregates in human models of breast cancer in vitro: insights into their mode of action
Authors: Irace Carlo , Misso Gabriella , Capuozzo Antonella , Piccolo Marialuisa , Riccardi Claudia , Luchini Alessandra , Caraglia Michele , Paduano Luigi , Montesarchio Daniela , Santamaria Rita 
Published: March 2017

AbstractLooking for new metal-based anticancer treatments, in recent years many ruthenium complexes have been proposed as effective and safe potential drugs. In this context we have recently developed a novel approach for the in vivo delivery of Ru(III) complexes, preparing stable ruthenium-based nucleolipidic nanoaggregates endowed with significant antiproliferative activity. Herein we describe the cellular response to our ruthenium-containing formulations in selected models of human breast cancer. By in vitro bioscreens in the context of preclinical studies, we have focused on their ability to inhibit breast cancer cell proliferation by the activation of the intrinsic apoptotic pathway, possibly via mitochondrial perturbations involving Bcl-2 family members and predisposing to programmed cell death. In addition, the most efficient ruthenium-containing cationic nanoaggregates we have hitherto developed are able to elicit both extrinsic and intrinsic apoptosis, as well as autophagy. To limit chemoresistance and counteract uncontrolled proliferation, multiple cell death pathways activation by metal-based chemotherapeutics is a challenging, yet very promising strategy for targeted therapy development in aggressive cancer diseases, such as triple-negative breast cancer with limited treatment options. These outcomes provide valuable, original knowledge on ruthenium-based candidate drugs and new insights for future optimized cancer treatment protocols.
TTF-1- and/or CD56-positive Circulating Tumor Cells in patients with small cell lung cancer (SCLC)
Authors: Messaritakis Ippokratis , Stoltidis Dimitris , Kotsakis Athanasios , Dermitzaki Eleftheria-Kleio , Koinis Fillipos , Lagoudaki Eleni , Koutsopoulos Anastasios , Politaki Eleni , Apostolaki Stella , Souglakos John , Georgoulias Vassilis 
Published: March 2017

AbstractThe aim of the study was to evaluate the phenotypic CTCs heterogeneity (TTF-1+ and/or CD56+) in SCLC patients and correlate it with the CellSearch. Peripheral blood was obtained from 108 consecutive patients. CTCs were detected by CellSearch and double-immunofluorescence using anti-CD45, anti-TTF-1 and anti-CD56 antibodies. Before chemotherapy TTF-1+/CD45−, CD56+/CD45− and TTF-1+/CD56+ CTCs were detected in 66(61.1%), 55(50.9%) and 46(42.6%) patients, respectively; 60.2% of patients were CellSearch+. Among the 22 patients with 0 CTCs/7.5 ml on CellSearch, TTF-1+/CD45−, CD56+/CD45− and TTF-1+/CD56+ CTCs were detected in 8(36.4%), 6(27.3) and 6(27.3%) patients, respectively; no CK+/EpCAM+ or TTF1+/EpCAM+ CTCs were detected in these patients. One-chemotherapy cycle decreased both the number of positive patients (p < 0.001) and their CTC number (p < 0.001), irrespectively of their phenotype and the detection method. The incidence and number of the different CTC subpopulations on PD, was significantly increased at their baseline levels. Multivariate analysis revealed that the increased number of CTCs at baseline and on PD were significantly associated with decreased PFS (p = 0.048) and OS (p = 0.041), respectively. There is an important CTC heterogeneity in such patients according to the expression of TTF-1 and CD56 which could detect EpCAM− CTC subpopulations and, thus, undetectable by CellSearch. These CTC subpopulations are dynamically correlated with treatment efficacy and disease-progression.
Laser beam melting 3D printing of Ti6Al4V based porous structured dental implants: fabrication, biocompatibility analysis and photoelastic study
Authors: Yang Fei , Chen Chen , Zhou QianRong , Gong YiMing , Li RuiXue , Li ChiChi , Klämpfl Florian , Freund Sebastian , Wu XingWen , Sun Yang , Li Xiang , Schmidt Michael , Ma Duan , Yu YouCheng 
Published: March 2017

AbstractFabricating Ti alloy based dental implants with defined porous scaffold structure is a promising strategy for improving the osteoinduction of implants. In this study, we use Laser Beam Melting (LBM) 3D printing technique to fabricate porous Ti6Al4V dental implant prototypes with three controlled pore sizes (200, 350 and 500 μm). The mechanical stress distribution in the surrounding bone tissue is characterized by photoelastography and associated finite element simulation. Forin-vitrostudies, experiments on implants’ biocompatibility and osteogenic capability are conducted to evaluate the cellular response correlated to the porous structure. As the preliminary results, porous structured implants show a lower stress-shielding to the surrounding bone at the implant neck and a more densed distribution at the bottom site compared to the reference implant. From the cell proliferation tests and the immunofluorescence images, 350 and 500 μm pore sized implants demonstrate a better biocompatibility in terms of cell growth, migration and adhesion. Osteogenic genes expression of the 350 μm group is significantly increased alone with the ALP activity test. All these suggest that a pore size of 350 μm provides an optimal provides an optimal potential for improving the mechanical shielding to the surrounding bones and osteoinduction of the implant itself.
E3 Ubiquitin Ligase Nedd4 Promotes Japanese Encephalitis Virus Replication by Suppressing Autophagy in Human Neuroblastoma Cells
Authors: Xu Qingqiang , Zhu Naiwei , Chen Shenglin , Zhao Ping , Ren Hao , Zhu Shiying , Tang Hailin , Zhu Yongzhe , Qi Zhongtian 
Published: March 2017

AbstractJapanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes the most prevalent viral encephalitis in Asia. Since JEV is a neurotropic virus, it is important to identify key molecules that mediate JEV infection in neuronal cells and to investigate their underlying mechanisms. In this study, the critical role of Nedd4, an E3 ubiquitin ligase that is highly expressed in the central nervous system, was examined in JEV propagation. In SK-N-SH neuroblastoma cells, Nedd4 was up-regulated in response to JEV infection. Moreover, down-regulation of Nedd4 resulted in a significant decrease in JEV replication without alterations in virus attachment and internalization or in JEV pseudotyped virus infection, suggesting that Nedd4 participates in the replication but not in the entry stage of JEV infection. Further functional analysis showed that Nedd4 attenuated JEV-induced autophagy, which negatively regulates virus replication during infection. These results suggest that Nedd4 facilitates the replication of JEV by suppressing virus-induced autophagy. Taken together, our results indicate that Nedd4 plays a crucial role in JEV infection of neuronal cells, which provides a potential target for the development of novel treatment to combat JEV infection.
The nicotinic acetylcholine receptor α7 subunit is an essential negative regulator of bone mass
Authors: Mito Kazuaki , Sato Yuiko , Kobayashi Tami , Miyamoto Kana , Nitta Eriko , Iwama Atsushi , Matsumoto Morio , Nakamura Masaya , Sato Kazuki , Miyamoto Takeshi 
Published: March 2017

AbstractThe nicotinic receptor α7nAchR reportedly regulates vagal nerve targets in brain and cardiac tissue. Here we show thatnAchR7−/−mice exhibit increased bone mass due to decreased osteoclast formation, accompanied by elevated osteoprotegerin/RANKL ratios in serum. Vagotomy in wild-type mice also significantly increased the serum osteoprotegerin/RANKL ratio, and elevated bone mass seen innAchR7−/−mice was reversed in α7nAchR/osteoprotegerin-doubly-deficient mice. α7nAchR loss significantly increased TNFα expression in Mac1-positive macrophages, and TNFα increased the osteoprotegerin/RANKL ratio in osteoblasts. Targeting TNFα innAchR7−/−mice normalized both serum osteoprotegerin/RANKL ratios and bone mass. Administration of nicotine, an α7nAchR ligand, to wild-type mice increased serum RANKL levels. Thus, vagal nerve stimulation of macrophages via α7nAchR regulates bone mass by modulating osteoclast formation.
Observation of low-loss broadband supermode propagation in coupled acoustic waveguide complex
Authors: Shen Ya-Xi , Peng Yu-Gui , Chen Xin-Cheng , Zhao De-Gang , Zhu Xue-Feng 
Published: March 2017

AbstractWe investigate analytically, numerically, and experimentally the low-loss supermode propagation in a coupled acoustic waveguide complex within a broadband. The waveguide complex is implemented with air channels coupled via an ultrathin metafluid layer. We analytically derive the field distribution of incident sound needed for producing acoustic supermodes, and verify the periodically revival propagation in coupled waveguide systems numerically and experimentally. We find out that the supermode wavelength becomes longer for higher mode order or lower frequency. We have also demonstrated the robust propagation of supermodes in broadband. Our scheme can in principle be extended to three dimensions and the ultrasound regime with simplicity and may promote applications of high-fidelity signal transfer in complicated acoustic networks.
Assessing the Amount of Spontaneous Real-World Spoken Language in Aphasia: Validation of Two Methods
Authors: Haddad Michelle M. , Taub Edward , Uswatte Gitendra , Johnson Margaret L. , Mark Victor W. , Barghi Ameen , Byrom Ezekiel , Zhou Xiaohua , Rodriguez Christina M. 
Published: March 2017

Purpose
The purpose of this article is to present the results of a study evaluating the psychometric properties of 2 new measures that exclusively assess the amount of real-world spoken language in patients with aphasia.

Method
Forty individuals with aphasia were evaluated on several measures of spoken language in real-world settings. The Verbal Activity Log (VAL; Johnson et al., 2014) has participants, aided by caregivers, indicate current amount and quality of real-world spoken language compared with before stroke. In addition, digital voice recorders objectively measured the amount of real-world spoken language. The Communicative Effectiveness Index (Lomas et al., 1989), a previously validated measure of functional communication, was used as a comparison measure. Nineteen participants received follow-up assessment ≥ 3 weeks later.

Results

Validity was supported by Pearson correlations between spoken language recordings and the VAL,
r
(38) = .70,
p <
.001. Likewise, correlation with the Communicative Effectiveness Index was strong,
r
(38) = .73,
p <
.001. Test–retest reliability for both VAL and audio recording was high, with intraclass correlations ≥ .96 and .90, respectively.

Conclusions
These results present preliminary evidence for the reliability and validity of the VAL and spoken language recording for assessment of the amount of real-world spoken language in aphasia. As a simple patient-reported outcome, the VAL may assist diverse therapies for aphasia.
CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells
Authors: Sheinberger Jonathan , Hochberg Hodaya , Lavi Erez , Kanter Itamar , Avivi Shira , Reinitz Gita , Schwed Avital , Aizler Yuval , Varon Eli , Kinor Noa , Shav-Tal Yaron 
Published: March 2017

AbstractDiscriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endogenous gene by creation of a new exon. Previously, CD-tagging was used to tag endogenous proteins. Here we developed a CD-tagging-MS2 approach in which two tags were inserted in tandem; a fluorescent protein tag in conjunction with the mRNA MS2 tag used for tagging mRNAs in cells. A cell clone library of CD-tagged-MS2 genes was generated, and protein and mRNA distributions were examined and characterized in single cells. Taking advantage of having one allele tagged, we demonstrate how the transcriptional activity of all alleles, tagged and untagged, can be identified using single molecule RNA fluorescence in situ hybridization (smFISH). Allele-specific mRNA expression and localization were quantified under normal and stress conditions. The latter generate cytoplasmic stress granules (SGs) that can store mRNAs, and the distribution of the mRNAs within and outside of the SGs was measured. Altogether, CD-tagging-MS2 is a robust and inexpensive approach for direct simultaneous detection of an endogenous mRNA and its translated protein product in the same cell.
Testing the Einstein's equivalence principle with polarized gamma-ray bursts
Authors: Yang Chao , Zou Yuan-Chuan , Zhang Yue-Yang , Liao Bin , Lei Wei-Hua 
Published: March 2017

Abstract
The Einstein's equivalence principle can be tested by using parametrized post-Newtonian parameters, of which the parameter γ has been constrained by comparing the arrival times of photons with different energies. It has been constrained by a variety of astronomical transient events, such as gamma-ray bursts (GRBs), fast radio bursts as well as pulses of pulsars, with the most stringent constraint of Δγ ≲ 10−15. In this Letter, we consider the arrival times of lights with different circular polarization. For a linearly polarized light, it is the combination of two circularly polarized lights. If the arrival time difference between the two circularly polarized lights is too large, their combination may lose the linear polarization. We constrain the value of Δγp &lt; 1.6 × 10−27 by the measurement of the polarization of GRB 110721A, which is the most stringent constraint ever achieved.
Early Hearing Detection and Intervention-Pediatric Audiology Links to Services EHDI-PALS: Building a National Facility Database
Authors: Chung Winnie , Beauchaine Kathryn L. , Hoffman Jeff , Coverstone Kirsten R. , Oyler Anne , Mason Craig 
Published: March 2017

Objectives:
To create a searchable web-based national audiology facility directory using a standardized survey, so parents and providers could identify which facilities had capacity to provide appropriate services based on child’s age.

Design:
An Early Hearing Detection and Intervention-Pediatric Audiology Links to Services expert panel was convened to create a survey to collect audiology facility information. Professional practice documents were reviewed, a survey was designed to collect pertinent test protocols of each audiology facility, and a standard of care template was created to cross-check survey answers. Audiology facility information across the United States was collected and compiled into a directory structured and displayed in an interactive website, ehdipals.org.

Results:
Since November 7, 2012, to May 21, 2016, over 1000 facilities have completed the survey and become listed in the Early Hearing Detection and Intervention-Pediatric Audiology Links to Services directory. The site has registered 10,759 unique visitors, 151,981 page views, and 9134 unique searches from consumers. User feedback has been positive overall.

Conclusion:
A searchable, web-based facility directory has proven useful to consumers as a tool to help them differentiate whether a facility was set up to test newborns versus young children. Use of a preprogrammed standard of practice template to cross-check survey answers was also shown to be a practical aid.
The Use of Bowel Protocols in Critically Ill Adult Patients: A Systematic Review and Meta-Analysis
Authors: Oczkowski Simon J. W. , Duan Erick H. , Groen Amy , Warren Dawn , Cook Deborah J. 
Published: March 2017

Objective:
Constipation is common among critically ill patients and has been associated with adverse patient outcomes. Many ICUs have developed bowel protocols to treat constipation; however, their effect on clinical outcomes remains uncertain. We conducted a systematic review to determine the impact of bowel protocols in critically ill adults.

Data Sources:
We searched MEDLINE, Embase, CINAHL, CENTRAL, ISRCTN, ClinicalTrials.gov, and conference abstracts until January 2016.

Study Selection:
Two authors independently screened titles and abstracts for randomized controlled trials comparing bowel protocols to control (placebo, no protocol, or usual care) in critically ill adults.

Data Extraction:
Two authors independently, and in duplicate, extracted study characteristics, outcomes, assessed risk of bias, and appraised the quality of evidence using the Grading of Recommendations Assessment, Development, and Evaluation approach.

Data Synthesis:
We retrieved 4,520 individual articles, and excluded 4,332 articles during title and abstract screening and 181 articles during full-text screening. Four trials, including 534 patients, were eligible for analysis. The use of a bowel protocol was associated with a trend toward a reduction in constipation (risk ratio, 0.50 [95% CI, 0.25–1.01]; p = 0.05; low-quality evidence); no reduction in tolerance of enteral feeds (risk ratio, 0.94 [95% CI, 0.62–1.42]; p = 0.77; low-quality evidence), and no change in the duration of mechanical ventilation (mean difference, 0.01 d [95% CI, –2.67 to 2.69 d]; low-quality evidence).

Conclusions:
Large, rigorous, randomized control trials are needed to determine whether bowel protocols impact patient-important outcomes in critically ill adults.