bluuebunny/crossref_metadata_2025_split huggingface dataset:
Programming of donor T cells using allogeneic δ-like ligand 4–positive dendritic cells to reduce GVHD in mice
Authors: Mochizuki Kazuhiro , Meng Lijun , Mochizuki Izumi , Tong Qing , He Shan , Liu Yongnian , Purushe Janaki , Fung Henry , Zaidi M. Raza , Zhang Yanyun , Reshef Ran , Blazar Bruce R. , Yagita Hideo , Mineishi Shin , Zhang Yi 
Published: May 2016

Key PointsEstablishing a novel Dll4hiDC-based programming approach that produces alloreactive T cells able to eliminate leukemic cells without GVHD. Dll4 induction of T-cell IFN-γ limits the expansion of Dll4hiDC-induced T cells in GVHD target tissues and development of GVHD.
HCK is a survival determinant transactivated by mutated MYD88, and a direct target of ibrutinib
Authors: Yang Guang , Buhrlage Sara J. , Tan Li , Liu Xia , Chen Jie , Xu Lian , Tsakmaklis Nicholas , Chen Jiaji G. , Patterson Christopher J. , Brown Jennifer R. , Castillo Jorge J. , Zhang Wei , Zhang Xiaofeng , Liu Shuai , Cohen Philip , Hunter Zachary R. , Gray Nathanael , Treon Steven P. 
Published: May 2016

Key PointsHCK transcription and activation is triggered by mutated MYD88, and is an important determinant of pro-survival signaling. HCK is also a target of ibrutinib, and inhibition of its kinase activity triggers apoptosis in mutated MYD88 cells.
readat: An R package for reading and working with SomaLogic ADAT files
Authors: Cotton Richard J. , Graumann Johannes 
Published: May 2016

AbstractBackgroundSomaLogic’s SOMAscan™ assay platform allows the analysis of the relative abundance of over 1300 proteins directly from biological matrices such as blood plasma and serum. The data resulting from the assay is provided in a proprietary text-based format not easily imported into R.Resultsreadatis an R package for working with the SomaLogic file format. It provides functionality for importing, transforming and annotating data from these files. The package is free, open source, and available on Bioconductor and Bitbucket.Conclusionsreadatintegrates into both Bioconductor and traditional R workflows, rendering it easy to make use of files.
QUADrATiC: scalable gene expression connectivity mapping for repurposing FDA-approved therapeutics
Authors: O’Reilly Paul G. , Wen Qing , Bankhead Peter , Dunne Philip D. , McArt Darragh G. , McPherson Suzanne , Hamilton Peter W. , Mills Ken I. , Zhang Shu-Dong 
Published: May 2016

Abstract
Background
Gene expression connectivity mapping has proven to be a powerful and flexible tool for research. Its application has been shown in a broad range of research topics, most commonly as a means of identifying potential small molecule compounds, which may be further investigated as candidates for repurposing to treat diseases. The public release of voluminous data from the Library of Integrated Cellular Signatures (LINCS) programme further enhanced the utilities and potentials of gene expression connectivity mapping in biomedicine.

Results
We describe QUADrATiC (http://go.qub.ac.uk/QUADrATiC), a user-friendly tool for the exploration of gene expression connectivity on the subset of the LINCS data set corresponding to FDA-approved small molecule compounds. It enables the identification of compounds for repurposing therapeutic potentials. The software is designed to cope with the increased volume of data over existing tools, by taking advantage of multicore computing architectures to provide a scalable solution, which may be installed and operated on a range of computers, from laptops to servers. This scalability is provided by the use of the modern concurrent programming paradigm provided by the Akka framework. The QUADrATiC Graphical User Interface (GUI) has been developed using advanced Javascript frameworks, providing novel visualization capabilities for further analysis of connections. There is also a web services interface, allowing integration with other programs or scripts.

Conclusions
QUADrATiC has been shown to provide an improvement over existing connectivity map software, in terms of scope (based on the LINCS data set), applicability (using FDA-approved compounds), usability and speed. It offers potential to biological researchers to analyze transcriptional data and generate potential therapeutics for focussed study in the lab. QUADrATiC represents a step change in the process of investigating gene expression connectivity and provides more biologically-relevant results than previous alternative solutions.
Estimation of rock physics properties from seismic attributes — Part 1: Strategy and sensitivity analysis
Authors: Dupuy Bastien , Garambois Stéphane , Virieux Jean 
Published: May 2016

The quantitative estimation of rock physics properties is of great importance in any reservoir characterization. We have studied the sensitivity of such poroelastic rock physics properties to various seismic viscoelastic attributes (velocities, quality factors, and density). Because we considered a generalized dynamic poroelastic model, our analysis was applicable to most kinds of rocks over a wide range of frequencies. The viscoelastic attributes computed by poroelastic forward modeling were used as input to a semiglobal optimization inversion code to estimate poroelastic properties (porosity, solid frame moduli, fluid phase properties, and saturation). The sensitivity studies that we used showed that it was best to consider an inversion system with enough input data to obtain accurate estimates. However, simultaneous inversion for the whole set of poroelastic parameters was problematic due to the large number of parameters and their trade-off. Consequently, we restricted the sensitivity tests to the estimation of specific poroelastic parameters by making appropriate assumptions on the fluid content and/or solid phases. Realistic a priori assumptions were made by using well data or regional geology knowledge. We found that (1) the estimation of frame properties was accurate as long as sufficient input data were available, (2) the estimation of permeability or fluid saturation depended strongly on the use of attenuation data, and (3) the fluid bulk modulus can be accurately inverted, whereas other fluid properties have a low sensitivity. Introducing errors in a priori rock physics properties linearly shifted the estimations, but not dramatically. Finally, an uncertainty analysis on seismic input data determined that, even if the inversion was reliable, the addition of more input data may be required to obtain accurate estimations if input data were erroneous.
A Common CD36 Variant Influences Endothelial Function and Response to Treatment with Phosphodiesterase 5 Inhibition
Authors: Shibao Cyndya A. , Celedonio Jorge E. , Ramirez Claudia E. , Love-Gregory Latisha , Arnold Amy C. , Choi Leena , Okamoto Luis E. , Gamboa Alfredo , Biaggioni Italo , Abumrad Naji N. , Abumrad Nada A. 
Published: May 2016

Context:
The scavenger receptor CD36 influences the endothelial nitric oxide-cGMP pathway in vitro. Genetic variants that alter CD36 level are common in African Americans (AAs), a population at high risk of endothelial dysfunction.

Objective:
To examine if the minor allele (G) of coding CD36 variant rs3211938 (G/T) which reduces CD36 level by approximately 50% influences endothelial function, insulin sensitivity (IS), and the response to treatment with the nitric oxide-cGMP potentiator sildenafil.

Design:
IS (frequently sampled iv glucose tolerance) and endothelial function (flow mediated dilation [FMD]) were determined in age- and body mass index-matched obese AA women with or without the G allele of rs3211938 (protocol 1). Effect of chronic sildenafil treatment on IS and FMD was tested in AA women with metabolic syndrome and with/without the CD36 variant, using a randomized, placebo-controlled trial (protocol 2).

Setting:
Two-center study.

Participants:
Obese AA women.

Intervention:
A total of 20-mg sildenafil citrate or placebo thrice daily for 4 weeks.

Main outcome:
IS, FMD.

Results:
G allele carriers have lower FMD (P = .03) and cGMP levels (P = .01) than noncarriers. Sildenafil did not improve IS, mean difference 0.12 (95% confidence interval [CI], −0.33 to 0.58; P = .550). However, there was a significant interaction between FMD response to sildenafil and rs3211938 (P = .018). FMD tended to improve in G carriers, 2.9 (95% CI, −0.9 to 6.8; P = .126), whereas it deteriorated in noncarriers, −2.6 (95% CI, −5.1 to −0.1; P = .04).

Conclusions:
The data document influence of a common genetic variant on susceptibility to endothelial dysfunction and its response to sildenafil treatment.
Activation of PI3K signaling prevents aminoglycoside-induced hair cell death in the murine cochlea
Authors: Jadali Azadeh , Kwan Kelvin Y. 
Published: May 2016

ABSTRACT
Loss of sensory hair cells of the inner ear due to aminoglycoside exposure is a major cause of hearing loss. Using an immortalized multipotent otic progenitor (iMOP) cell line, specific signaling pathways that promote otic cell survival were identified. Of the signaling pathways identified, the PI3K pathway emerged as a strong candidate for promoting hair cell survival. In aging animals, components for active PI3K signaling are present but decrease in hair cells. In this study, we determined whether activated PI3K signaling in hair cells promotes survival. To activate PI3K signaling in hair cells, we used a small molecule inhibitor of PTEN or genetically ablated PTEN using a conditional knockout animal. Hair cell survival was challenged by addition of gentamicin to cochlear cultures. Hair cells with activated PI3K signaling were more resistant to aminoglycoside-induced hair cell death. These results indicate that increased PI3K signaling in hair cells promote survival and the PI3K signaling pathway is a target for preventing aminoglycoside-induced hearing loss.
Are current methods of partial weight-bearing instruction accurately translating to crutch-assisted gait?
Authors: Graham Claire S , Stephens Deana M , Dietz Kristina C , Winter Samantha L 
Published: May 2016

Background/Aims: Partial weight-bearing protocols are commonly incorporated into hospital, clinical and field-based rehabilitation to enhance recovery, particularly in patients following cartilage surgeries. Overloading can affect healing time and the stability or integrity of the healing structure, however underloading can also be detrimental, as adequate weight bearing encourages the healing process—for example, osteoblastic stimulation. Therefore, accurate reproducibility of these protocols could be considered essential to the rehabilitation process. The aim of this study was to determine the accuracy with which weight-bearing protocols (20%, 50% or 80% of body weight) could be reproduced shortly after being taught. Methods: Thirty participants were taught three partial weight-bearing protocols (20%, 50% and 80% of body weight), using bathroom scales. Participants ability to reproduce their target load for each protocol was assessed statically using bathroom scales and dynamically with a force plate using a three-point elbow crutch-assisted gait. Participants were assessed 10 minutes after being taught. Errors between actual and target load during these trials was calculated. Findings: Accuracy assessed with scales was comparatively good for all target loads, however dynamic trials using the force plate showed an inverse relationship between all error measures and target loads (i.e. 20% > 50% > 80% body weight; all P<0.01). The peak error was double the intended load at 20% of body weight (95% CI: 11.9% body weight, 24.1% body weight). At 80% of body weight, the peak error was not significantly different from zero. Conclusions: The static method of instruction of partial weight-bearing protocols, using bathroom scales, does not seem to translate accurately to dynamic motion, and therefore affects adherence to medical instruction. Practitioners should be aware of the potential errors in reproducing these loads and the potential effect on rehabilitation. These results would suggest that practitioners should be cautious when using bathroom scales to teach partial weight-bearing protocols and not to rely on them to assess reproduction accuracy during gait.
The National Diabetes Prevention Programme: Working with patients
Authors: Phillips Anne 
Published: May 2016

Type 2 diabetes is the fastest growing health issue in the UK. The National Diabetes Prevention Programme aims to stem this tide, as Anne Phillips explains It is important that those at risk of type 2 diabetes are identified before the disease develops and medication is required in order to reduce risk—practice nurses are ideally placed to be at the forefront of identification. This article investigates the introduction of the UK National Diabetes Prevention Programme and examines the evidence from previous programmes, all of which have confirmed the need for a concerted effort to prevent type 2 diabetes through timely diagnosis of non-diabetic hyperglycaemia and referral to diabetes prevention interventions that are focused on behavioural changes. The importance of NHS Health Checks and opportunities for primary and secondary intervention measures are also stressed.
Endometriosis: The role practice nurses can play
Authors: Norton Wendy , Holloway Debby 
Published: May 2016

Endometriosis is a condition that affects around 1 in 10 women in the UK. Despite this prevalence, diagnosis is often delayed. Wendy Norton and Debby Holloway explain the role of the practice nurse in supporting women with this condition Endometriosis is a chronic condition where endometrial tissue forms lesions outside the uterus, resulting in internal bleeding, inflammation, fibrosis, and adhesion formation. It is estimated that as many as one in 10 women in the UK have this painful condition. The signs and symptoms of endometriosis vary in their severity and presentation, increasing difficulties with diagnosis. Earlier diagnosis and support may help women to manage the condition and its impact on their day-to-day lives. Endometriosis has physical, psychological and sexual health impacts on women's—and their partners'—lives. Practice nurses are ideally placed to support women with endometriosis.
Post-homophobia comes out: The rise of Mormon polygamy in US popular culture
Authors: Bailey Courtney W. , Zahren Adam James 
Published: May 2016

Abstract
The past decade has witnessed significant gains for gay men and lesbians in the United States, a development lauded as liberal progress and decried as an infringement on religious rights. Meanwhile, a Mormon polygamy cottage industry that addresses cultural anxieties about sexuality and religion has appeared in US media. Although these two trends may seem only slightly related, we argue that they herald the dawn of ‘post-homophobia’ as a discursive framework for incorporating the legacy of LGBTQ+ social movements. This article analyses how both Sister Wives and news coverage of the Fundamentalist Church of Jesus Christ of Latter-Day Saints (FLDS) police who can become an intelligible US citizen and on what sexual and religious grounds. Although post-homophobia makes space for alternative familial arrangements, it recentres white, middle-class, Christian heterosexuality as the rightful heirs to sexual minority politics. Under the guise of familial pluralism and religious freedom, such portrayals ultimately shore up mono/heteronormativity and the neo-Orientalism that undergirds the US War on Terrorism.
Interrogating transnational documentary film evidence on Uganda’s homophobia
Authors: Atuhura Dorothy 
Published: May 2016

Abstract
The over conspicuousness of Uganda’s homophobia in international media of the twenty-first century is highly commendable for foregrounding the severe abuse of human rights of homosexuals in Uganda. Although this medium establishes its space as an agent of human rights activism and as a record of what happened to homosexuals in Uganda, it is important to interrogate the process involved in producing it, as well as what this mediated index forecloses as it signifies the severe abuse of human rights of homosexuals in Uganda. Focusing on three filmic documentaries on Uganda’s homophobia, I argue this media hypervisibility is a global phenomenon – a transnational media collective implicated in rebranding and networking historical transnational processes, tropes and ideologies that have not only produced conditions necessary for homophobia to thrive in Uganda, but also established Uganda’s anti-homosexuality violence as the new signature of Africa’s ‘Darkness’.
Numerical simulations of airflow in the human pharynx of OSAHS patients
Authors: Kluck C. , Buzug T.M. 
Published: May 2016

Abstract
Abstract: Computational Fluid Dynamic simulations are performed in real patient individual pharynx geometries of an Obstructive Sleep Apnea patient. The Navier-Stokes equations as well as the Reynolds Averaged Navier-Stokes equations and k − ∊ and k −ω turbulence models are used. The velocity profile and pressure distribution of the patient without any treatment and the patient wearing a mandibular advancement appliance are compared to each other. The simulation results for the different model conditions all lead to similar results showing the robustness of the numerical solutions. The pressure loss along the pharynx is lower in the presence of a mandibular appliance, which can indicate the reduction of OSAHS severity.
Green chemistry preparation of MgO grit like nanostructures: efficient catalyst for the synthesis of 4H-pyrans and α,α′-bis(substituted-benzylidene) cycloalkanone derivatives
Authors: Mobinikhaledi Akbar , Yazdanipour Atisa , Ghashang Majid 
Published: May 2016

Abstract
A grit like nanostructure of MgO was prepared in a medium of mulberry leaves extract and characterized on the basis of field emission scanning electronic microscopy (FE-SEM), X-ray diffraction (XRD), and energy dispersive X-ray (EDX) analysis. It was found to be an efficient catalyst for one-pot synthesis of 4H-pyrans from the reaction of malononitrile and α,α′-bis(substituted-benzylidene) cycloalkanones in good to high yields.
Role of reverse transendothelial migration of neutrophils in inflammation
Authors: Hirano Yohei , Aziz Monowar , Wang Ping 
Published: May 2016

Abstract
Transmigration of neutrophils through vascular endothelial walls into the inflamed tissues is a critical defense mechanism of innate immune system against infection and injury caused by sepsis, trauma, ischemia-reperfusion, and other acute or chronic inflammatory diseases. However, their excessive infiltration and uncontrolled activation may lead to the destruction of normal tissue architecture and unrestrained inflammation. Transendothelial migration (TEM) in a luminal-to-abluminal direction is widely known as the final step of neutrophil migration cascade into the inflamed tissues. Recent studies have shown that neutrophils not necessarily move from the vascular lumen to the extravascular tissues in a one way direction; they also proceed in an opposite direction, known as reverse transendothelial migration (rTEM) to get back into the vascular lumen again. This novel paradigm of neutrophil round trip is currently on the spotlight due to its possible interaction with immune system. Current review highlighting the growing demand of this newly identified neutrophil migratory event will not only rewrite the disease pathophysiology, but also help scientists design novel therapeutic strategy leading to the remission of inflammatory diseases in which controlling exaggerated neutrophil infiltration is a major challenge.
Controlling quality and amount of mitochondria by mitophagy: insights into the role of ubiquitination and deubiquitination
Authors: Tan Tao , Zimmermann Marcel , Reichert Andreas S. 
Published: May 2016

Abstract
Mitophagy is a selective autophagy pathway conserved in eukaryotes and plays an essential role in mitochondrial quality and quantity control. Mitochondrial fission and fusion cycles maintain a certain amount of healthy mitochondria and allow the isolation of damaged mitochondria for their elimination by mitophagy. Mitophagy can be classified into receptor-dependent and ubiquitin-dependent pathways. The mitochondrial outer membrane protein Atg32 is identified as the only known receptor for mitophagy in baker’s yeast, whereas mitochondrial proteins FUNDC1, NIX/BNIP3L, BNIP3 and Bcl2L13 are recognized as mitophagy receptors in mammalian cells. Earlier studies showed that ubiquitination and deubiquitination occurs in yeast, yet there is no direct evidence for an ubiquitin-dependent mitophagy pathway in this organism. In contrast, a ubiquitin-/PINK1-/Parkin-dependent mitophagy pathway was unraveled and was extensively characterized in mammals in recent years. Recently, a quantitative method termed synthetic quantitative array (SQA) technology was developed to identify modulators of mitophagy in baker’s yeast on a genome-wide level. The Ubp3-Bre5 deubiquitination complex was found as a negative regulator of mitophagy while promoting other autophagic pathways. Here we discuss how ubiquitination and deubiquitination regulates mitophagy and other selective forms of autophagy and what argues for using baker’s yeast as a model to study the ubiquitin-dependent mitophagy pathway.
Biological and biochemical characteristics of a Mediterranean population with Gestational Diabetes Mellitus
Authors: Savona-Ventura Charles , Vassallo Josanne , Craus Johann , Anastasiou Eleni , Jotic Aleksandra , Lalic Nebojsa M. , Slama Claude Ben , Loscos Adele Rovira , Napoli Angela , Roma Giona 
Published: May 2016

AbstractThe interplay of various nutrients provided to the developing foetus determines the growth potential of the conceptus. This study assessed the inter-relationship between these nutrients in a Mediterranean population including 1062 pregnant, previously non-diabetic women. These underwent an oral glucose tolerance test (oGTT) and were accordingly classified into gestational hyperglycaemic and normoglycaemic groups. Fasting insulin, HbA1c, and lipid profiles were further assessed, and the anthropomorphic characteristics of the mother and child at birth were measured. Lipid profiles were compared between the two groups and related to the biological characteristics of the mother and child at birth. Gestational hyperglycaemia was significantly associated with elevated triglycerides (P<0.0001) and decreased low density lipoprotein cholesterol (LDL-C) (P=0.02). There were no significant changes in total cholesterol and high density lipoprotein cholesterol (HDL-C) levels. Maternal BMI correlated positively with the various glycaemic indices (P<0.0001) and triglycerides (P<0.0001), but inversely with cholesterol (P<0.0001), HDL-C (P<0.0001) and LDL-C (P<0.0001). The infant birth weight correlated positively with maternal body weight (P<0.0001), LDL-C (P<0.0001) and the glycaemic indices (P<0.0001), but negatively with cholesterol (P<0.0001), triglycerides (P<0.0001), HDL-C (P<0.0001) and FBG (P<0.0001). This study confirms that the maternal body mass index (BMI), insulin resistance, and LDL-C levels positively contribute towards foetal growth, whereas a negative correlation was noted with cholesterol, triglycerides, and HDL-C.
Is omitting the 3rd hour measurement in the 100 g oral glucose tolerance test feasible?
Authors: Şükür Yavuz Emre , Seval Mehmet Murat , Özmen Batuhan , Yalçin İbrahim , Karaeren Zihni , Söylemez Feride , Koç Acar 
Published: May 2016

AbstractTo evaluate the diagnostic value of the 3Records of all pregnant patients with abnormal 50 g glucose challenge test (GCT) between January 2005 and December 2013 were reviewed (n=1963). The 100 g OGTT results were analyzed separately for both Carpenter & Couston (CC) and National Diabetes Data Group (NDDG) criteria.The number of patients diagnosed with gestational diabetes mellitus (GDM) was 297 (15.1%) according to CC criteria and 166 (8.4%) according to NDDG criteria. The 1Omitting 3