bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supramalleolar Osteotomy With or Without Fibular Osteotomy for Varus Ankle Arthritis
Authors: Hongmou Zhao , Xiaojun Liang , Yi Li , Hongliang Liu , Junhu Wang , Cheng Liu 
Published: May 2016

Background: Supramalleolar osteotomy (SMOT) is an alternative operative procedure for the management of early and midstage varus ankle arthritis. However, whether fibular osteotomy is needed is controversial. The purpose of the current study was to evaluate the functional and radiologic outcomes of pre- and postoperative SMOT, and to compare the outcomes between patients with and without fibular osteotomy. Methods: Forty-one Takakura stage 2 and 3 varus ankle osteoarthritis patients treated with SMOT were included. Fourteen males and 27 females with a mean age of 50.7 (range, 32-71) years were followed with a mean of 36.6 (range, 17-61) months. There were 22 cases with fibular osteotomy and 19 without. The American Orthopaedic Foot & Ankle Society (AOFAS) ankle-hindfoot score, Maryland foot score, and Ankle Osteoarthritis Score (AOS) were used for pre- and postoperative functional evaluation. The tibial articular surface angle (TAS), talar tilt (TT), tibiocrural angle (TC), and tibial lateral surface angle (TLS) were evaluated pre- and postoperatively. Results: At the last follow-up, the mean AOFAS score (from 50.8 to 83.1 points) and Maryland score (from 58.3 to 81.6 points) in overall were improved ( P < .01); the mean AOS pain (from 42.6 to 26.1 points) and function (from 53.4 to 36.8 points) scores were decreased ( P < .01). For radiologic evaluation, all the included parameters were improved ( P < .05) except TLS. The mean Takakura stage was decreased ( P < .01). No significant difference could be detected in comparing the functional outcomes between those with and without fibular osteotomy. However, in the fibular osteotomy group, TT was decreased ( P < .05) and TC was improved ( P < .01) significantly. Conclusion: SMOT was promising, with substantial functional improvement and malalignment correction for varus ankle arthritis. Fibular osteotomy may be necessary in cases with large TT and small TC angles. Level of Evidence: Level III, retrospective comparative study.
Radiographic and CT Assessment of Reduction of Calcaneus Fractures Using a Limited Sinus Tarsi Incision
Authors: Scott Aaron T. , Pacholke David A. , Hamid Kamran S. 
Published: May 2016

Background: The lateral extensile incision for fixation of displaced intra-articular calcaneus fractures allows for fracture reduction but has been associated with high rates of soft tissue complications. This has prompted a search for less invasive methods of fracture fixation. The purpose of the present study was to determine the adequacy of reduction and rate of complications associated with operative fixation of calcaneal fractures using a limited sinus tarsi approach. Methods: A limited sinus tarsi incision with plate fixation was utilized for treatment of 39 displaced intra-articular calcaneal fractures in 35 consecutive patients as part of a single surgeon series. Imaging assessment of previously described fracture displacement measures was undertaken in preoperative and postoperative radiographs and CT. A retrospective chart review was conducted to identify postoperative complications. Results: Mean preoperative Bohler angle measurement was 7.7 (range, –26.0 to 30.0) degrees and the mean final postoperative standing Bohler angle was 25.5 (range, 12.3 to 37.7) degrees. Postoperative CT demonstrated that subtalar articular reduction was within 2 mm of anatomic in 91% of patients. There were 2 instances of superficial wound dehiscence (5.1%) and 1 deep infection (2.6%) that required debridement and complete hardware removal. Visual analog score (VAS) for pain averaged 3 of 10 in the 32 available patients at 1-year follow-up. Eight of these patients (25%) reported no pain (0/10) at final follow-up. Conclusion: Operative fixation of displaced intra-articular calcaneal fractures utilizing the limited sinus tarsi approach resulted in acceptable fracture reduction and a low rate of complications. Level of Evidence: Level IV, retrospective case series.
Does the diameter of abdominal aortic aneurysm influence late survival following abdominal aortic aneurysm repair? A systematic review and meta-analysis
Authors: Khashram Manar , Hider Phil N , Williman Jonathan A , Jones Gregory T , Roake Justin A 
Published: May 2016

Background Studies reporting the influence of preoperative abdominal aortic aneurysm diameter on late survival following abdominal aortic aneurysm repair have not been consistent. Aim: To report the influence of abdominal aortic aneurysm diameter on overall long-term survival following abdominal aortic aneurysm repair. Methods Embase, Medline and the Cochrane electronic databases were searched to identify articles reporting the influence of abdominal aortic aneurysm diameter on late survival following open aneurysm repair and endovascular aneurysm repair published up to April 2015. Data were extracted from multivariate analysis; estimated risks were expressed as hazard ratio. Results A total of 2167 titles/abstracts were retrieved, of which 76 studies were fully assessed; 19 studies reporting on 22,104 patients were included. Preoperative larger abdominal aortic aneurysm size was associated with a worse survival compared to smaller aneurysms with a pooled hazard ratio of 1.14 (95% CI: 1.09–1.18), per 1 cm increase in abdominal aortic aneurysm diameter. Subgroup analysis of the different types of repair was performed and the hazard ratio (95% CI), for open aneurysm repair and endovascular aneurysm repair were 1.08 (1.03–1.12) and 1.20 (1.15–1.25), respectively, per 1 cm increase. There was a significant difference between the groups p < 0.02. Conclusions This meta-analysis suggests that preoperative large abdominal aortic aneurysm independently influences overall late survival following abdominal aortic aneurysm repair, and this association was greater in abdominal aortic aneurysm repaired with endovascular aneurysm repair.
Low Levels of Hemoglobin at Admission Are Associated With Increased 30-Day Mortality in Patients With Hip Fracture
Authors: Praetorius Katrine , Madsen Christian M. , Abrahamsen Bo , Jørgensen Henrik L. , Lauritzen Jes B. , Laulund Anne Sofie B. 
Published: May 2016

Introduction: Previous smaller studies suggest that anemia is a risk factor for mortality in patients with hip fracture. The purpose of this investigation was to assess the correlation between hemoglobin at admission with 30-day mortality following a hip fracture in a large-scale study. Patients and Methods: From January 1996 to December 2012, all patients with hip fracture (>60 years of age) admitted to Bispebjerg Hospital, Copenhagen, were identified from a local hip fracture database. We excluded conservatively treated patients and patients who died preoperatively. Results: Seven thousand four hundred twenty-one consecutive patients with hip fracture were identified. Of those 7319 had a hemoglobin measurement on admission and were thus eligible for further analysis. Mean hemoglobin for patients alive at 30 days was 7.6 (standard deviation [SD]: 1.0) and for deceased patients 7.4 (SD: 1.1), P < .0001. Mean age was 82.6 years (SD: 8.5), and 76.5% of the population were female (Nfemales = 5600). The 30-day mortality decreases for every increase in hemoglobin of 1.0 mmol/L in a univariate analysis ( P < .0001). The hazard ratio (HR) with 95% confidence interval (CI) for 30-day mortality in patients with anemia (<7.3 mmol/L for females and <8.3 mmol/L for males; Nanemic = 3235) was 1.66 (CI: 1.43-1.91, P < .0001). Adjusting for age, type of fracture, gender, and comorbidities (Charlson score) slightly attenuated the risk estimate (HR: 1.21, CI: 1.03-1.41, P = .02). Conclusion: This study demonstrates increased 30-day mortality in patients with low hemoglobin at admission, even after adjusting for comorbidities.
A novel microfluidic assay reveals a key role for protein kinase C δ in regulating human neutrophil–endothelium interaction
Authors: Soroush Fariborz , Zhang Ting , King Devon J , Tang Yuan , Deosarkar Sudhir , Prabhakarpandian Balabhaskar , Kilpatrick Laurie E , Kiani Mohammad F 
Published: May 2016

Abstract
A key step in neutrophil-mediated tissue damage is the migration of activated neutrophils across the vascular endothelium. Previously, we identified protein kinase C δ as a critical regulator of neutrophil migration in sepsis but did not identify specific steps in migration. In this study, we used our novel biomimetic microfluidic assay to delineate systematically the mechanism by which protein kinase C δ regulates individual steps in human neutrophil–endothelial interaction during inflammation. The biomimetic microfluidic assay includes a network of vascular channels, produced from in vivo images connected to a tissue compartment through a porous barrier. HUVECs cultured in vascular channels formed a complete lumen under physiologic shear flow. HUVECs were pretreated with TNF-α ± a protein kinase C δ inhibitor, and the tissue compartment was filled with a chemoattractant (fMLP or IL-8). Under physiologic shear flow, the role of protein kinase C δ on spatial and temporal neutrophil adherence/migration was quantified. Protein kinase C δ inhibition significantly reduced neutrophil adhesion in response to fMLP and IL-8 only under low shear rate and near bifurcations. Protein kinase C δ inhibition also decreased adherence to nonactivated HUVECs in response to fMLP or IL-8. Protein kinase C δ inhibition reduced neutrophil migration into the tissue compartment in response to fMLP and to a lesser degree, to IL-8. Antibody-coated microparticles demonstrated that protein kinase C δ inhibition down-regulated E-selectin and ICAM-1 but not VCAM-1 expression. With the use of a physiologically relevant in vitro model system, we demonstrate that protein kinase C δ plays an important role in the regulation of neutrophil adherence/migration during inflammation and identifies key steps regulated by protein kinase C δ in neutrophil–endothelial interactions.
Circulating T helper and T regulatory subsets in untreated early rheumatoid arthritis and healthy control subjects
Authors: Pandya Jayesh M , Lundell Anna-Carin , Hallström Magnus , Andersson Kerstin , Nordström Inger , Rudin Anna 
Published: May 2016

AbstractThe pathogenic role and frequency of T cell subtypes in early rheumatoid arthritis are still unclear. We therefore performed a comprehensive analysis of the circulating T cell subtype pattern in patients with untreated early rheumatoid arthritis compared to healthy control subjects. Peripheral blood mononuclear cells were obtained from 26 patients with untreated early rheumatoid arthritis and from with 18 age- and sex-matched healthy control subjects. T helper cell types Th0, Th1, Th2, Th17, and Th1/17 and nonclassic T helper subsets were defined by flow cytometry based on the expression of chemokine receptors CCR4, CCR6, and CXCR3. Regulatory T cells were defined by expression of CD25+ CD127low and also FOXP3. CXCR5+ cells among regulatory and nonregulatory T cells were defined as T follicular regulatory and T follicular helper cells, respectively. The phenotype of T cell subsets was confirmed by transcription factor and cytokine secretion analyses. Multivariate discriminant analysis showed that patients with untreated early rheumatoid arthritis were segregated from healthy control subjects based on the circulating T cell subset profile. Among the discriminator subsets, CCR4+CXCR3− (Th2 and Th17), CTLA4+ and FOXP3+ subsets were present in significantly higher frequencies, whereas CCR4− (Th1/Th17, CCR6+CCR4−CXCR3−, and Th1) subsets were present in lower frequencies in patients with untreated early rheumatoid arthritis compared with healthy control subjects. The proportions of Th2 and Th17 subsets associated positively with each other and negatively with the CXCR3+/interferon γ-secreting subsets (Th1 and Th1/Th17) in patients with untreated rheumatoid arthritis. The proportions of Th2 cells increased with age in patients with untreated early rheumatoid arthritis and healthy control subjects. The dominance of circulating CCR4+CXCR3– T helper subsets (Th2 and Th17) in untreated early rheumatoid arthritis point toward a pathogenic role of these cells in early stages of the disease.
The protease inhibitor cystatin C down-regulates the release of IL-β and TNF-α in lipopolysaccharide activated monocytes
Authors: Gren Susanne Thiesen , Janciauskiene Sabina , Sandeep Salipalli , Jonigk Danny , Kvist Peter Helding , Gerwien Jens Gammeltoft , Håkansson Katarina , Grip Olof 
Published: May 2016

Abstract
Human cystatin C, a member of the cysteine proteinase-inhibitory family, is produced by all nucleated cells and has important roles in regulating natural immunity. Nematode homologs to human cystatin C have been shown to have anti-inflammatory effects on monocytes and to reduce colitis in mice. In Crohn’s disease, pathogenic activated monocytes help drive inflammatory processes via the release of proinflammatory cytokines and chemokines. In particular, tumor necrosis factor-α–producing inflammatory monocytes have a central role in the intestinal inflammation in patients with Crohn’s disease. We investigated the potential of human cystatin C to regulate pathogenic activated monocytes and its potential as an Immunomodulator in Crohn’s disease. We found that cystatin C significantly decreased the lipopolysaccharide-stimulated release and expression of interleukin-1β and tumor necrosis factor-α in monocyte and peripheral blood mononuclear cell cultures from healthy donors, whereas interleukin-6 and interleukin-8 levels were unchanged. A similar reduction of interleukin-1β and tumor necrosis factor-α was also seen in peripheral blood mononuclear cell cultures from patients with Crohn’s disease, and in particular, tumor necrosis factor-α was reduced in supernatants from lamina propria cell cultures from patients with Crohn’s disease. Further investigation revealed that cystatin C was internalized by monocytes via an active endocytic process, decreased phosphorylation of the mitogen-activated protein kinase pathway extracellular signal-regulated kinase-1/2, and altered surface marker expression. The ability of cystatin C to modulate the cytokine expression of monocytes, together with its protease-inhibitory function, indicates that modulation of the local cystatin C expression could be an option in future Crohn’s disease therapy.
OPA1 processing in cell death and disease – the long and short of it
Authors: MacVicar Thomas , Langer Thomas 
Published: May 2016

ABSTRACT
The regulation of mitochondrial dynamics by the GTPase OPA1, which is located at the inner mitochondrial membrane, is crucial for adapting mitochondrial function and preserving cellular health. OPA1 governs the delicate balance between fusion and fission in the dynamic mitochondrial network. A disturbance of this balance, often observed under stress and pathologic conditions, causes mitochondrial fragmentation and can ultimately result in cell death. As discussed in this Commentary, these morphological changes are regulated by proteolytic processing of OPA1 by the inner-membrane peptidases YME1L (also known as YME1L1) and OMA1. Long, membrane-bound forms of OPA1 are required for mitochondrial fusion, but their processing to short, soluble forms limits fusion and can facilitate mitochondrial fission. Excessive OPA1 processing by the stress-activated protease OMA1 promotes mitochondrial fragmentation and, if persistent, triggers cell death and tissue degeneration in vivo. The prevention of OMA1-mediated OPA1 processing and mitochondrial fragmentation might thus offer exciting therapeutic potential for human diseases associated with mitochondrial dysfunction.
Kinematics of mouthbrooding inOreochromis niloticus(Cichlidae)
Authors: Van Wassenbergh Sam , Joris Iris , Desclée Mathieu , Liew Hon Jung , De Boeck Gudrun , Adriaens Dominique , Aerts Peter 
Published: May 2016

ABSTRACTMany species from several different families of fishes perform mouthbrooding, where one of the sexes protects and ventilates the eggs inside the mouth cavity. This ventilation behaviour differs from gill ventilation outside the brooding period, as the normal, small-amplitude suction-pump respiration cycles are alternated with actions including near-simultaneous closed-mouth protrusions and high-amplitude depressions of the hyoid. The latter is called churning, referring to its hypothetical function in moving around and repositioning the eggs by a presumed hydrodynamic effect of the marked shifts in volume along the mouth cavity. We tested the hypothesis that churning causes the eggs located posteriorly in the mouth cavity to move anteriorly away from the gill entrance. This would prevent or clear accumulations of brood at the branchial basket, which would otherwise hinder breathing by the parent. Dual-view videos of female Nile tilapias (Oreochromis niloticus) during mouthbrooding showed that churning involves a posterior-to-anterior wave of expansion and compression of the head volume. Flow visualisation with polyethylene microspheres revealed a significant inflow of water entering the gill slits at the zone above the pectoral fin base, followed by a predominantly ventral outflow passing the ventrolaterally flapping branchiostegal membranes. X-ray videos indicated that particularly the brood located close to the gills is moved anteriorly during churning. These data suggest that, in addition to mixing of the brood to aid its oxygenation, an important function of the anterior flow through the gills and buccal cavity during churning is to prevent clogging of the eggs near the gills.
Theoretical Study of the Mechanism for Direct Addition of Hydride to CO2 on Ruthenium Complexes: Nature of Ru–H Bond and Effect of Hydrogen Bonding
Authors: Li Jun , Liu Sha , Lu Xiaohua 
Published: May 2016

Abstract
In this work, we study the hydrogenation of CO2 via the direct addition of hydride to CO2 on Ru(II) complexes to make clear the influences of two key factors, the nature of Ru–H bond and the effect of hydrogen bonding, on CO2 insertion into the Ru–H bond. The density functional theory calculation results show that: (1) There exist two types of Ru–H bonds. If the Ru–H bond is formed from the mixing of the sd3 hybrid orbital of Ru with the 1s orbital of H, the hydride cannot be directly added to CO2 even with the help of hydrogen bonding. The direct hydride addition to CO2 only can occur for the hydride involved in a Ru–H bond formed from the mixing of the sd2 hybrid orbital of Ru with the 1s orbital of H. (2) For active hydride, hydrogen bonding can facilitate the migration of hydride to CO2. (3) If less d orbital component is involved in the formation of the Ru–H bond on similar complexes, the hydride will be more active. In the light of these laws, the activity of the hydride on a Ru(II) complex might be adjusted by the change of the ligand trans to the hydride.
New Formulations for the Conflict Resolution Problem in the Scheduling of Television Commercials
Authors: Giallombardo Giovanni , Jiang Houyuan , Miglionico Giovanna 
Published: May 2016

We consider the conflict-resolution problem arising in the allocation of commercial advertisements to television program breaks. Because of the competition-avoidance requirements issued by advertisers, broadcasters aim to allocate any pairs of commercials promoting highly conflicting products to different breaks. Hence, the problem consists of assigning commercials to breaks, subject to time capacity constraints, with the aim of maximizing a total measure of the conflicts among commercials assigned to different breaks. Since the existing formulation can hardly be solved via exact methods, we introduce three new and efficient (mixed-)integer programming formulations of the problem. Our computational study is based on two sets of test problems, one from the literature and another more challenging data set that we generate. Numerical results show the excellent performance of the proposed formulations in terms of solution quality and computation times, when compared against an existing formulation and an effective heuristic approach. In particular, for the existing data set, all three formulations significantly outperform the existing formulation and heuristic, and moreover, for the new data set, our best formulation outperforms the heuristic on 76% of the test examples in terms of solution quality. We also provide theoretical evidence to demonstrate why some of our new formulations should outperform the existing formulation.
Preparation, characterization of a ceria loaded carbon nanotubes nanocomposites photocatalyst and degradation of azo dye Acid Orange 7
Authors: Wen Tao , Tang Yu-bin , Chen Fang-yan , Mo Bing-yu 
Published: May 2016

Abstract
A ceria loaded carbon nanotubes (CeO2/CNTs) nanocomposites photocatalyst was prepared by chemical precipitation, and the preparation conditions were optimized using an orthogonal experiment method. HR-TEM, XRD, UV-Vis/DRS, TGA and XPS were used to characterize the photocatalyst. Nitrogen adsorption-desorption was employed to determine the BET specific surface area. The results indicated that the photocatalyst has no obvious impurities. CeO2 was dispersed on the carbon nanotubes with a good loading effect and high loading efficiency without agglomeration. The catalyst exhibits a strong ability to absorb light in the ultraviolet region and some ability to absorb light in the visible light region. The CeO2/CNTs nanocomposites photocatalyst was used to degrade azo dye Acid Orange 7 (40 mg/L). The optical decolorization rate was 66.58% after xenon lamp irradiation for 4 h, which is better than that of commercial CeO2 (43.13%). The results suggested that CeO2 loading on CNTs not only enhanced the optical decolorization rate but also accelerated the separation of CeO2/CNTs and water.
Response surface methodology (RSM) and its application for optimization of ammonium ions removal from aqueous solutions by pumice as a natural and low cost adsorbent
Authors: Moradi Masoud , Fazlzadehdavil Mehdi , Pirsaheb Meghdad , Mansouri Yadollah , Khosravi Touba , Sharafi Kiomars 
Published: May 2016

Abstract
This research was conducted to study the adsorption of ammonium ions onto pumice as a natural and low-cost adsorbent. The physico-chemical properties of the pumice granular were characterized by X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Modeling and optimization of a NH4+ sorption process was accomplished by varying four independent parameters (pumice dosage, initial ammonium ion concentration, mixing rate and contact time) using a central composite design (CCD) under response surface methodology (RSM). The optimum conditions for maximum removal of NH4+ (70.3%) were found to be 100 g, 20 mg/l, 300 rpm and 180 min, for pumice dosage, initial NH4+ ion concentration, mixing rate and contact time. It was found that the NH4+ adsorption on the pumice granular was dependent on adsorbent dosage and initial ammonium ion concentration. NH4+ was increased due to decrease the initial concentration of NH4 and increase the contact time, mixing rate and amount of adsorbent.