bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon as an immunoadjuvant to enhance antibodies following influenza B infection and vaccination in ferrets
Authors: Rowe Thomas , Fletcher Ashley , Svoboda Pavel , Pohl Jan , Hatta Yasuko , Jasso Gabriela , Wentworth David E. , Ross Ted M. 
Published: October 2024

AbstractDespite annual vaccination, influenza B viruses (IBV) continue to cause significant morbidity and mortality in humans. We have found that IBV infection resulted in a weaker innate and adaptive immune response than influenza A viruses (IAV) in ferrets. To understand and overcome the weak immune responses to IBV in ferrets, we administered type-I or type-III interferon (IFN) to ferrets following infection or vaccination and evaluated their effects on the immune response. IFN signaling following viral infection plays an important role in the initial innate immune response and affects subsequent adaptive immune responses. In the respiratory tract, IFN lambda (IFNL) has regulatory effects on adaptive immunity indirectly through thymic stromal lymphopoietin (TSLP), which then acts on immune cells to stimulate the adaptive response. Following IBV infection or vaccination, IFN treatment (IFN-Tx) upregulated gene expression of early inflammatory responses in the upper respiratory tract and robust IFN, TSLP, and inflammatory responses in peripheral blood cells. These responses were sustained following challenge or vaccination in IFN-Tx animals. Serum IFNL and TSLP levels were enhanced in IFN-Tx animals following challenge/rechallenge over mock-Tx; however, this difference was not observed following vaccination. Antibody responses in serum of IFN-Tx animals following IBV infection or vaccination increased more quickly and to higher titers and were sustained longer than mock-Tx animals over 3 months. Following rechallenge of infected animals 3 months post treatment, antibody levels remained higher than mock-Tx. However, IFN-Tx did not have an effect on antibody responses following challenge of vaccinated animals. A strong direct correlation was found between TSLP levels and antibody responses following challenge-rechallenge and vaccination-challenge indicating it as a useful tool for predicting adaptive immune responses following IBV infection or vaccination. The effects of IFN on strengthening both innate and adaptive responses to IBV may aid in development of more effective treatments following infection and improved influenza vaccines.
Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis
Authors: Pfeil-Gardiner Olivia , Rosa Higor Vinícius Dias , Riedel Dietmar , Chen Yu Seby , Lörks Dominique , Kükelhan Pirmin , Linck Martin , Müller Heiko , Van Petegem Filip , Murphy Bonnie J. 
Published: October 2024

AbstractFor macromolecular structures determined by cryogenic electron microscopy, no technique currently exists for mapping elements to defined locations, leading to errors in the assignment of metals and other ions, cofactors, substrates, inhibitors and lipids that play essential roles in activity and regulation. Elemental mapping in the electron microscope is well established for dose-tolerant samples but is challenging for biological samples, especially in a cryo-preserved state. Here we combine electron energy-loss spectroscopy with single-particle image processing to allow elemental mapping in cryo-preserved macromolecular complexes. Proof-of-principle data show that our method, reconstructed electron energy-loss (REEL) analysis, allows a three-dimensional reconstruction of electron energy-loss spectroscopy data, such that a high total electron dose is accumulated across many copies of a complex. Working with two test samples, we demonstrate that we can reliably localize abundant elements. We discuss the current limitations of the method and potential future developments.
A comprehensive Beyond-GDP database to accelerate wellbeing, inclusion, and sustainability research
Authors: Liu Kedi , Wang Ranran , Behrens Paul , Schrijver Inge , Jansen Annegeke , A. Rum Irlan , Hoekstra Rutger 
Published: October 2024

Abstract“Beyond-GDP” metrics are essential for understanding societal progress. Yet despite their importance, these metrics are scattered across various databases, hindering accessibility and interdisciplinary analysis. Addressing this gap, we present the ‘WISE database’ – the first extensive collection of important Beyond-GDP metrics organized by Wellbeing, Inclusion, and Sustainability (WISE) dimensions. The WISE database consolidates data from a variety of sources, including international institutions and academic publications. It encompasses over one million data points across 244 metrics, covering 218 countries and 61 country groupings, from specific social and environmental indicators to the main Beyond-GDP indexes, and is augmented by essential metadata. The data primarily spans from 1995 to 2015, with some metrics extending back to the 19th century. To improve accessibility and data interpretation, a user-friendly online visualization tool has been developed. The WISE database aims to foster a broader view of societal progress, facilitate research on synergies and trade-offs of wellbeing, inclusion, and sustainability, and provide a foundation for interdisciplinary research.
Association mapping unravels the genetic basis for drought related traits in different developmental stages of barley
Authors: Slawin Connor , Ajayi Oyeyemi , Mahalingam Ramamurthy 
Published: October 2024

AbstractDrought stress significantly reduces crop yields at all stages of plant development. Barley, known for its abiotic-stress adaptation among cereals was used to examine the genetic basis of drought tolerance. A population of 164 spring barley lines was subjected to polyethylene glycol (PEG) induced drought stress during germination and seedling development. Six traits were measured, including germination percentage and rate, seedling length and weight, and root-to-shoot ratios. Seedling area, volume, and root and shoot diameter was acquired with a flatbed scanner. This population was also subjected to short-term drought during the heading stage in the greenhouse. Root and shoot weight and grain yield data were collected from well watered and droughted plants. Significant variation within traits were observed and several of them exhibited strong correlations with each other. In this population, two genotypes had 100% germination under PEG-induced drought and drought tolerance throughout the heading stage of plant development. A genome-wide association scan (GWAS) revealed 64 significant marker-trait associations across all seven barley chromosomes. Candidate genes related to abiotic stress and germination were identified within a 0.5Mbp interval around these SNPs. In silico analysis indicated a high frequency of differential expression of the candidate genes in response to stress. This study enabled identification of barley lines useful for drought tolerance breeding and pinpointed candidate genes for enhancing drought resiliency in barley.
Carbon stocks in Norwegian eelgrass meadows across environmental gradients
Authors: Gagnon Karine , Thormar Jonas , Fredriksen Stein , Potouroglou Maria , Albretsen Jon , Gundersen Hege , Hancke Kasper , Rinde Eli , Wathne Cecilie , Norderhaug Kjell Magnus 
Published: October 2024

AbstractSeagrass meadows are well-known for their capacity to capture and store blue carbon in sediments. However carbon stocks vary significantly between meadows, spanning more than three orders of magnitude on both local and global scales. Understanding the drivers of seagrass carbon stocks could help improve strategies for incorporating blue carbon into management plans. Here, we measured sediment carbon stocks in eelgrass (Zostera marina) meadows and unvegetated areas along the Norwegian coast, spanning wide gradients in temperature, wave exposure, water depth, salinity, and eelgrass biomass. Carbon stocks were generally higher in eelgrass meadows than in adjacent unvegetated areas, yet they displayed considerable variation (400 − 30 000 g C m−2 at 50 cm sediment depth) even among nearby sites. Overall, the highest carbon stocks were found in deeper, muddier, sheltered meadows near river mouths. These sites likely have the highest input and retention of carbon from different sources. Consequently, they should be prioritized as conservation targets for preserving coastal blue carbon stocks. Despite ever-increasing efforts to quantify seagrass blue carbon globally, high uncertainties still persist, partly due to differing methodologies, processes, and environmental context. Blue carbon stock estimates could be improved through the coordination of standardised mapping and sampling methods.
Synthesis and characterization of ceramic refractories based on industrial wastes
Authors: Khater G. A. , Romero M. , López-Delgado A. , Padilla I. , El-Kheshen A. A. , Farag M. M. , Elmaghraby M. S. , Nasralla N. H. S. 
Published: October 2024

AbstractThe possibility of reusing ceramic roller waste to produce cordierite and mullite refractories was investigated. Five batches were designed using wastes representing ceramic roller waste, magnesite, and silica sand, shaped and fired at 1300 °C/2 h, and one batch was selected at 1200 °C. The chemical composition and precipitated phases of the used raw materials and the fired batches were analyzed using XRF and XRD techniques, respectively. Densification parameters, morphology, microstructure and electrical properties were also studied to evaluate the effect of the formed phases on the properties of fired materials. Bulk density increases with an increase in mullite and a decrease in cordierite, and it also increases with increasing temperature, whereas porosity and water absorption show a opposite behavior to density (it decreases with an increase in mullite and temperature). The main phases developed after firing at 1300 °C/2 h were cordierite, mullite, corundum, baddeleyite, and spinel. Bending strength increases with increasing mullite percentage and density, and decreasing grain size and porosity. The microstructure develops and becomes finer with increasing mullite percentage and density. The grain size of the crystals was very fine at 1200 °C/2 h and increased at 1300 °C/2 h. Broadband dielectric spectroscopy was employed to study the electrical and dielectric behavior of the investigated samples. The increase in mullite concentration shows a remarkable increase in ε’, especially at lower frequencies, as it is three times higher than that of M10. At f > 103 Hz ε’, frequency independence is accompanied by an increase in mullite concentrations due to the lag of dynamics fluctuations after the alteration of the electric field. The generation of new free ions leads to the enhancement of conductivity as the mullite concentration increases.
Recruitment of autophagy initiator TAX1BP1 advances aggrephagy from cargo collection to sequestration
Authors: Bauer Bernd , Idinger Jonas , Schuschnig Martina , Ferrari Luca , Martens Sascha 
Published: October 2024

AbstractAutophagy mediates the degradation of harmful material within lysosomes. In aggrephagy, the pathway mediating the degradation of aggregated, ubiquitinated proteins, this cargo material is collected in larger condensates prior to its sequestration by autophagosomes. In this process, the autophagic cargo receptors SQSTM1/p62 and NBR1 drive cargo condensation, while TAX1BP1, which binds to NBR1, recruits the autophagy machinery to facilitate autophagosome biogenesis at the condensates. The mechanistic basis for the TAX1BP1-mediated switch from cargo collection to its sequestration is unclear. Here we show that TAX1BP1 is not a constitutive component of the condensates. Its recruitment correlates with the induction of autophagosome biogenesis. TAX1BP1 is sufficient to recruit the TBK1 kinase via the SINTBAD adapter. We define the NBR1–TAX1BP1-binding site, which is adjacent to the GABARAP/LC3 interaction site, and demonstrate that the recruitment of TAX1BP1 to cargo mimetics can be enhanced by an increased ubiquitin load. Our study suggests that autophagosome biogenesis is initiated once sufficient cargo is collected in the condensates.
The dietary exposome: a brief history of diet, longevity, and age-related health in rodents
Authors: Selman Colin 
Published: October 2024

Abstract
It has been recognized for over a century that feeding animals less food than they would normally eat increases lifespan and leads to broad-spectrum improvements in age-related health. A significant number of studies have subsequently shown that restricting total protein, branched chain amino acids or individual amino acids in the diet, as well as ketogenic diets, can elicit similar effects. In addition, it is becoming clear that fasting protocols, such as time-restricted-feeding or every-other-day feeding, without changes in overall energy intake can also profoundly affect rodent longevity and late-life health. In this review, I will provide a historical perspective on various dietary interventions that modulate ageing in rodents and discuss how this understanding of the dietary exposome may help identify future strategies to maintain late-life health and wellbeing in humans.
Robust planar object tracking using SIFT and GHT with spatial locality
Authors: Lee Suwon , Choi Sang‐Min 
Published: October 2024

AbstractPlanar object tracking (POT) is crucial in vision‐based robotic applications. Despite significant advancements, tracking planar objects under real‐world conditions remains a challenge owing to various factors. The method proposed by the authors leverages SIFT features and generalized Hough transform, which is enhanced by spatial locality, to mitigate background clutter and false matching. The experimental results demonstrate that this method significantly outperforms existing approaches, achieving higher precision across various alignment error thresholds.
A novel online reinforcement learning‐based linear quadratic regulator for three‐level neutral‐point clamped DC/AC inverter
Authors: Qie Tianhao , Zhang Xinan , Xiang Chaoqun , Iu Herbert Ho Ching , Fernando Tyrone 
Published: October 2024

AbstractThis article proposes a novel online reinforcement learning‐based linear quadratic regulator for the three‐level neutral‐point clamped DC/AC voltage source inverter. The proposed controller employs online updated fixed‐weight recurrent neural network (NN) and policy iteration to dynamically adjust the optimal control gains based on real‐time measurements without any knowledge of the system model or offline pre‐training. Moreover, it produces a constant switching frequency with low current harmonics. Compared to the existing control methods, it provides superior control performance, guaranteed control stability, and simplified NN design. Experimental results are presented to verify the effectiveness of the proposed control method.
A novel vehicle power line communication method based on switching ripple modulation
Authors: Zhu He , Xie Zhiyuan , Cao Wangbin , Bai Zonglong , Hu Zhengwei 
Published: October 2024

AbstractPower converters can perform power conversion and data transmission simultaneously. With the aim of mitigating the hardware requirements of vehicle power line communication (VPLC), a novel VPLC scheme based on switching ripple modulation (SRM) is proposed to eliminate the need for coupling circuits and specialized communication chips. By embedding data modulation techniques into traditional pulse width modulation systems, power/data multiplexing transmission is achieved by demodulating the voltage ripple on the DC bus. First, the theoretical foundation of the SRM is introduced, and power/data single carrier modulation using 2FSK and data demodulation based on the sliding discrete Fourier transform are provided. Subsequently, comprehensive analyses of the mutual influence between power conversion and communication in the buck converter are conducted. The impact of power conversion on communication primarily depends on the control loop compensation and perturbation depth. Moreover, the designs of the ripple conditioning circuit and communication frame are presented. Finally, an experimental prototype is constructed with two buck converters in parallel. The experimental results demonstrate a data transmission rate of 12.5 kbps within 3 m under steady‐state, transient input and output voltage, and transient load conditions, validating the correctness of the proposed methodology.
Berufliche Stressoren und Ressourcen von
Genesungsbegleiter*innen/Peer-Berater*innen in der
Sozialpsychiatrie
Authors: Hoghe Jelena , Röseler Lukas , Limmer Ruth , Walther Christoph , Schütz Astrid 
Published: October 2024

Zusammenfassung
Ziel der Studie Erfasst wurden berufliche Stressoren und Ressourcen von
Peer Berater*innen (PB) und als wie belastend bzw. entlastend sie diese
empfinden.
Methode Fragebogengestützte Umfrage
Ergebnisse 175 PB wurden in die Datenanalyse einbezogen und nannten
Stressoren und Ressourcen in ihrem beruflichen Alltag. Psychologische Sicherheit
und Autonomie werden als am häufigsten vorkommende Ressourcen berichtet,
Rollenklarheit am seltensten. Autonomie wird als relativ wenig entlastend
eingestuft, sie kann in Zusammenhang mit Rollenunklarheit auch überfordern. Zwar
fühlen sich PB selten durch das Team stigmatisiert, wenn dies jedoch der Fall
ist, erleben sie die Stigmatisierung als sehr belastend. Relevante Stressoren
waren Unsicherheit in Entscheidungen, Probleme der Teamkultur oder als inadäquat
empfundene Rollenerfüllung durch das Team.
Schlussfolgerungen Obwohl relevante Ressourcen vorhanden sind, ist zu
konstatieren, dass Unklarheiten in den Arbeitsprozessen und den Rollen aller
Beteiligten bedeutsame Belastungen sind.
Wie belastbar ist Deine Diagnose?
Authors: Gruber Achim D. , Wehrend Axel , Steidl Thomas , Kramer Katharina , Merbach Sabine , Baumgärtner Wolfgang , Buyle Thomas , Weisser Niclas-Frederic , Beineke Andreas 
Published: October 2024

ZusammenfassungDie Effektivität und Rechtfertigung jeder Therapie und anderen klinischen
Entscheidung basieren auf einer korrekten Diagnose. Doch viele Arten von
Untersuchungsergebnissen können Unsicherheiten enthalten, die unter Umständen zu
klinischen Fehlentscheidungen führen können. Ähnliches gilt für die
Belastbarkeit von Gutachten für gerichtliche Auseinandersetzungen. Daher ist die
adäquate Mitteilung diagnostischer und gutachterlicher Unsicherheiten im
Untersuchungsbericht bzw. Sachverständigengutachten entscheidend für die
Vermeidung von Fehlentscheidungen. Auch die Haftbarkeit der Person, die die
Leistung erbringt, ist davon betroffen. Unsichere oder gar fehlerhafte Befunde
können jedoch diverse Ursachen haben, von denen nur ein Teil der untersuchenden
oder der beauftragenden Person bekannt sind. Dieser Beitrag bietet eine
Übersicht über 3 verschiedene Arten von Fehleranfälligkeiten am Beispiel
pathologischer Biopsie- und Zytologieuntersuchungen, die so oder ähnlich auch
auf andere tierärztliche Disziplinen übertragbar sind. Ein solides Verständnis
der möglichen Fehlerquellen sowie die adäquate Kommunikation und Diskussion
fallspezifischer, eingeschränkter Wahrscheinlichkeiten in Untersuchungsberichten
und Gutachten tragen wesentlich dazu bei, Fehlentscheidungen zu vermeiden. Bei
den üblicherweise eingesetzten Begriffen wie „mit hoher Wahrscheinlichkeit“,
„Verdacht auf“ oder „nicht auszuschließen“ bestehen jedoch teils unklare bis
abweichende Interpretationen, die hier mit Empfehlungen für einen einheitlichen
Gebrauch erläutert werden. Damit soll die entscheidende Person in die Lage
versetzt werden, im Kontext aller übrigen verfügbaren Daten nötigenfalls weitere
Diagnostik bzw. Beweiserhebung zu veranlassen, um die Irrtumswahrscheinlichkeit
so weit wie möglich zu reduzieren.
Nephrektomie bei einem Kaninchen aufgrund eines renalen
Adenokarzinoms
Authors: Engels Mona , Soutullo Iria Dominguez 
Published: October 2024

ZusammenfassungDer folgende Fallbericht beschreibt den klinischen Ansatz der Diagnostik und
Therapie eines Adenokarzinoms der Niere bei einem 2-jährigen Kaninchen, welches
initial aufgrund von Hyporexie und Polydipsie vorgestellt wurde. In der
klinischen Untersuchung zeigte sich palpatorisch eine abdominale
Umfangsvermehrung, die sich nach weiterführenden Untersuchungen als unilaterale,
für eine Neoplasie verdächtige renale Umfangsvermehrung herausstellte. Nach
einem Tumor-Staging mittels Blutuntersuchung, abdominaler Sonografie und
Röntgenaufnahmen des Thorax und Abdomens, ohne Hinweise auf Metastasen, erfolgte
eine Nephrektomie. Die pathohistologische Untersuchung ergab ein renales
Adenokarzinom. Nach Abheilen der Wunde war der Patient trotz der vorsichtigen
Prognose vollständig symptomfrei und zeigte auch 12 Monate nach der Operation
keine Metastasen oder weitere Einschränkungen.
Forschungsschwerpunkte und Qualität der wissenschaftlichen Literatur
zur Dystokie beim Rind von 1960–2020
Authors: Ulrich Lisa , Wehrend Axel 
Published: October 2024

ZusammenfassungIm Rahmen einer Literaturauswertung wurde mittels einer systematischen Recherche
ermittelt, wie sich in der deutsch- und englischsprachigen Literatur die
Forschungsschwerpunkte über die Schwergeburt beim Rind seit den 1960er Jahren
bis 2020 verändert haben und welche Qualität die bisherige wissenschaftliche
Literatur aufweist. Dazu wurden 621 Literaturstellen ausgewertet. Es zeigt sich,
dass die Anzahl der Veröffentlichungen zwischen 2010 und 2020 deutlich
zugenommen hat, was die anhaltende Aktualität der Thematik unterstreicht.
Fallserien (35,3%) stellen die häufigste Art der Publikation dar. Kontrollierte
Studien (4,5%) und Metaanalysen (0,3%) konnten deutlich seltener gefunden
werden. Die Ursachen (43,8%) und Folgen der Dystokie (28,8%) wurden bisher am
häufigsten untersucht, die Häufigkeit dagegen nur in wenigen Studien (7,2%).
Während Behandlungsmethoden vornehmlich von 1960 bis 1979 analysiert wurden,
lässt sich für die Themenbereiche „Ursachen der Dystokie“ und „Folgen der
Dystokie“ kein zeitlicher Schwerpunkt finden. Veröffentlichungen, die die
Prävention von Dystokie thematisieren, finden sich vermehrt ab den 1980er
Jahren.Zusammenfassend ist festzuhalten, dass Publikationen zur Ursachen der Dystokie
quantitativ derzeit die höchste Relevanz haben. Für zukünftige
Forschungsprojekte sollten die Studien so geplant werden, dass sie Grundlage für
systematische Studien, Übersichtsarbeiten und Metaanalysen sein können.
StäB vs. Station – Gibt es Unterschiede zwischen den beiden
Behandlungsformen im retrospektiven Verlauf hinsichtlich einzelner objektiver
und subjektiver Outcome-Variablen?
Authors: Raschmann Svenja , Eisele Frank , Flammer Erich , Längle Gerhard 
Published: October 2024

ZusammenfassungDiese retrospektive Verlaufsuntersuchung über ein Jahr (nach Entlassung aus der
Indexbehandlung) vergleicht die Effekte stationsäquivalenter (StäB) und
vollstationärer psychiatrischer Behandlung anhand subjektiver und objektiver
Qualitätsindikatoren. Über ein 1:1 Kohorten-Matching (Diagnose, Alter,
Geschlecht, Zahl der Vorbehandlungen und Krankheitsschwere) konnten 732
StäB-Patient*innen einer Vergleichsgruppe zugeordnet werden. Outcome-Daten
wurden den Routinedaten der Klinikdatenbank und der hausinternen
Basisdokumentation entnommen. Im subjektiven Qualitätsmaß gaben Patient*innen
und Behandelnde in stationärer Behandlung eine signifikant höhere
Zustandsverbesserung an. StäB Patient*innen wiesen deskriptiv häufiger eine
weitere Akutbehandlung auf, hinsichtlich des objektiven Qualitätsmaßes, der Zahl
der weiteren stationären Behandlungstage im Betrachtungszeitraum, unterschieden
sich die beiden Gruppen nicht signifikant, was als Ermutigung für den Einsatz
dieser neuen, sich zunehmend etablierenden Behandlungsform gedeutet werden
kann.