bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitroevaluation of a basic fibroblast growth factor‐containing hydrogel toward vocal fold lamina propria scar treatment
Authors: Erndt‐Marino Josh D. , Jimenez‐Vergara Andrea C. , Diaz‐Rodriguez Patricia , Kulwatno Jonathan , Diaz‐Quiroz Juan Felipe , Thibeault Susan , Hahn Mariah S. 
Published: June 2017

AbstractScarring of the vocal fold lamina propria can lead to debilitating voice disorders that can significantly impair quality of life. The reduced pliability of the scar tissue—which diminishes proper vocal fold vibratory efficiency—results in part from abnormal extracellular matrix (ECM) deposition by vocal fold fibroblasts (VFF) that have taken on a fibrotic phenotype. To address this issue, bioactive materials containing cytokines and/or growth factors may provide a platform to transition fibrotic VFF within the scarred tissue toward an anti‐fibrotic phenotype, thereby improving the quality of ECM within the scar tissue. However, for such an approach to be most effective, the acute host response resulting from biomaterial insertion/injection likely also needs to be considered. The goal of the present work was to evaluate the anti‐fibrotic and anti‐inflammatory capacity of an injectable hydrogel containing tethered basic fibroblast growth factor (bFGF) in the dual context of scar and biomaterial‐induced acute inflammation. Anin vitroco‐culture system was utilized containing both activated, fibrotic VFF and activated, pro‐inflammatory macrophages (MΦ) within a 3D poly(ethylene glycol) diacrylate (PEGDA) hydrogel containing tethered bFGF. Following 72 h of culture, alterations in VFF and macrophage phenotype were evaluated relative to mono‐culture and co‐culture controls. In our co‐culture system, bFGF reduced the production of fibrotic markers collagen type I, α smooth muscle actin, and biglycan by activated VFF and promoted wound‐healing/anti‐inflammatory marker expression in activated MΦ. Cumulatively, these data indicate that bFGF‐containing hydrogels warrant further investigation for the treatment of vocal fold lamina propria scar. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1258–1267, 2018.
Efficacy of cytapheresis for remission induction and dermatological manifestations of ulcerative colitis
Authors: Nomura Osamu , Osada Taro , Shibuya Tomoyoshi , Ishikawa Dai , Haga Keiichi , Kodani Tomohiro , Sakamoto Naoto , Ogihara Tatsuo , Yamaji Ken , Watanabe Sumio 
Published: June 2017

AbstractBackgroundIn ulcerative colitis (UC) patients, cytapheresis depletes elevated and activated leucocytes, which are known to release inflammatory cytokines including tumor necrosis factor (TNF)‐α. Further, there are UC patients who develop erythema nodosum (EN) or pyoderma gangrenosum (PG) as extra‐intestinal manifestations of UC.MethodsBetween 2008 and 2015, 181 consecutive patients with active UC received cytapheresis with either a granulocyte and monocyte apheresis (GMA) column or with a leucocyte removal filter (LCAP) as remission induction therapy. Each patient received weekly or intensive (2‐3 sessions/week) cytapheresis up to 10 sessions. In 13 patients, UC was complicated by EN or PG. Lichtiger's clinical activity index (CAI) ≤4 meant remission, while ≥3 decrease in CAI meant response to therapy. Prednisolone sparing and the changes in the extra‐intestinal manifestations were factored for assessing treatment efficacy.ResultsThe overall remission and response rates were 52.5% and 71.8%, respectively, CAI fell from 9.4 ± 3.3 to 4.9 ± 3.5 (P < 0.001). The efficacy rates in subgroups on concomitant corticosteroid, anti‐TNF or tacrolimus, and those without concomitant medications were not significantly different (P > 0.05). However, in 84 patients on prednisolone, the average daily prednisolone dose was reduced from 18.15 to 12.43 mg/day (P < 0.001) with 21.7% being corticosteroid free. All patients with EN or PG showed favorable response to cytapheresis, notably 2 EN patients achieving remission after just 2 cytapheresis sessions without concomitant medication.ConclusionsIn this retrospective efficacy evaluation, cytapheresis was effective as remission induction therapy with steroid sparing effect and desirable safety profile. Further, patients with EN or PG responded favorably to cytapheresis.
Physicochemical properties of some hydrophobic room‐temperature ionic liquids applied to volatile organic compounds biodegradation processes
Authors: Rodriguez Castillo Alfredo Santiago , Guihéneuf Solène , Biard Pierre‐François , Paquin Ludovic , Amrane Abdeltif , Couvert Annabelle 
Published: June 2017

AbstractBACKGROUNDIonic liquids (IL) are an interesting solvent choice for specific industrial applications since physicochemical properties can be fine‐tuned by modifying the substituent groups or the cation/anion pair. Hydrophobic ILs are considered green solvents and known to be good absorbents for hydrophobic organic compounds. Given their physicochemical properties an industrial application of such compounds is conceivable. Classical physicochemical properties such as density, viscosity and surface tension have a strong influence on the fluid dynamics; they were therefore measured and determined.RESULTSThe density, viscosity and surface tension of 23 hydrophobic ILs were measured at room temperature. These compounds are potential candidates for the absorption and biodegradation of volatile organic compounds (VOCs) in a two‐phase partitioning bioreactor. The thermal expansion coefficient, molecular volume, standard molar entropy and lattice energy were determined for each IL using empirical and semi‐empirical equations based on the density values. Viscosity values were correlated by the Arrhenius equation. Then, the surface excess enthalpy and surface excess entropy were determined from the surface tension values.CONCLUSIONThe influence of the presence of different functional moieties (unsaturated bonding, oxygenated and cyanide) and the side chain length in the physicochemical properties of these hydrophobic ILs was discussed, since their presence affected directly the density, viscosity and surface tension. © 2017 Society of Chemical Industry
Refining of wine lees and cheese whey for the production of microbial oil, polyphenol‐rich extracts and value‐added co‐products
Authors: Kopsahelis Nikolaos , Dimou Charalampia , Papadaki Aikaterini , Xenopoulos Evangelos , Kyraleou Maria , Kallithraka Stamatina , Kotseridis Yorgos , Papanikolaou Seraphim , Koutinas Apostolis A 
Published: June 2017

AbstractBACKGROUNDRefining of renewable resources for the production of various end‐products should be applied in order to develop sustainable processes and ensure the transition to the bio‐economy era. With this principle in mind, a novel integrated biorefinery has been developed based on cheese whey and wine lees valorization.RESULTSPolyphenols, tartrate salts and ethanol were extracted from wine lees, while the remaining solids enriched in yeast cells were converted into nutrient‐rich fermentation supplements. The composition of phenolics varied between solid and liquid fractions of wine lees. Protein concentrate was separated from cheese whey via ultrafiltration, while the concentrated lactose‐rich permeate stream was supplemented with wine lees derived hydrolysates to form fermentation media for microbial oil production by Cryptococcus curvatus and Mortierella ramanniana. Functional oil containing 4.5% (w/w) of the omega‐6 fatty acid γ‐linolenic acid was produced in shake flask cultures by Mortierella ramanniana with total dry weight of 25.8 g L−1 and 30.6% (w/w) lipid content. Fed‐batch bioreactor cultures with Cryptococcus curvatus using only crude resources led to one of the highest lipid concentrations (33.1 g L−1) reported in literature‐cited publications using cheese whey.CONCLUSIONThis is the first study proposing the integrated refining of cheese whey and wine lees for the production of both commodity and speciality products, namely whey protein concentrate, antioxidants, ethanol, tartrate salts and microbial oil. © 2017 Society of Chemical Industry
MR techniques for guiding high‐intensity focused ultrasound (HIFU) treatments
Authors: Kuroda Kagayaki 
Published: June 2017

To make full use of the ability of magnetic resonance (MR) to guide high‐intensity focused ultrasound (HIFU) treatment, effort has been made to improve techniques for thermometry, motion tracking, and sound beam visualization. For monitoring rapid temperature elevation with proton resonance frequency (PRF) shift, data acquisition and processing can be accelerated with parallel imaging and/or sparse sampling in conjunction with appropriate signal processing methods. Thermometry should be robust against tissue motion, motion‐induced magnetic field variation, and susceptibility change. Thus, multibaseline, referenceless, or hybrid techniques have become important. In cases with adipose or bony tissues, for which PRF shift cannot be used, thermometry with relaxation times or signal intensity may be utilized. Motion tracking is crucial not only for thermometry but also for targeting the focus of an ultrasound in moving organs such as the liver, kidney, or heart. Various techniques for motion tracking, such as those based on an anatomical image atlas with optical‐flow displacement detection, a navigator echo to seize the diaphragm position, and/or rapid imaging to track vessel positions, have been proposed. Techniques for avoiding the ribcage and near‐field heating have also been examined. MR acoustic radiation force imaging (MR‐ARFI) is an alternative to thermometry that can identify the location and shape of the focal spot and sound beam path. This technique could be useful for treating heterogeneous tissue regions or performing transcranial therapy. All of these developments, which will be discussed further in this review, expand the applicability of HIFU treatments to a variety of clinical targets while maintaining safety and precision.Level of Evidence: 2Technical Efficacy: Stage 4J. Magn. Reson. Imaging 2018;47:316–331.
Nuclei‐specific deposits of iron and calcium in the rat thalamus after status epilepticus revealed with quantitative susceptibility mapping (QSM)
Authors: Aggarwal Manisha , Li Xu , Gröhn Olli , Sierra Alejandra 
Published: June 2017

PurposeTo investigate pathological changes in the rat brain after pilocarpine‐induced status epilepticus using quantitative susceptibility mapping (QSM).Materials and Methods3D multiecho gradient‐echo (GRE) data were acquired from ex vivo brains of pilocarpine‐injected and age‐matched control rats at 11.7T. Maps of R2* and quantitative susceptibility were calculated from the acquired 3D GRE magnitude and phase data, respectively. QSM and R2* maps were compared with Perls' (iron) and Alizarin‐red‐S (calcium) stainings in the same brains to investigate the pathophysiological basis of susceptibility contrast.ResultsBilaterally symmetric lesions were detected in reproducible thalamic regions of pilocarpine‐treated rats, characterized by hyperintensity in R2* maps. In comparison, quantitative susceptibility maps demonstrated heterogeneous contrast within the lesions, with distinct hyperintense (paramagnetic) and hypointense (diamagnetic) areas. Comparison with histological assessment revealed localized deposits of iron‐ and calcium‐positive granules in thalamic nuclei corresponding to paramagnetic and diamagnetic areas delineated in the susceptibility maps, respectively. Pronounced differences were observed in the lesions between background‐corrected phase images and reconstructed susceptibility maps, indicating unreliable differentiation of iron and calcium deposits in phase maps. Multiple linear regression showed a significant association between susceptibility values and measured optical densities (ODs) of iron and calcium in the lesions (R2 = 0.42, P < 0.001), with a positive dependence on OD of iron and negative dependence on OD of calcium.ConclusionQSM can detect and differentiate pathological iron and calcium deposits with high sensitivity and improved spatial accuracy compared to R2* or GRE phase images, rendering it a promising technique for diagnosing thalamic lesions after status epilepticus.Level of Evidence: 1Technical Efficacy: Stage 1J. Magn. Reson. Imaging 2018;47:554–564.
Impact of microvascular obstruction on left ventricular local remodeling after reperfused myocardial infarction
Authors: Zhang Lin , Mandry Damien , Chen Bailiang , Huttin Olivier , Hossu Gabriela , Wang Hairong , Beaumont Marine , Girerd Nicolas , Felblinger Jacques , Odille Freddy 
Published: June 2017

PurposeTo evaluate by cardiac magnetic resonance imaging (MRI) the impact of microvascular obstruction (MVO) on regional left ventricular (LV) wall characteristics and local remodeling after acute myocardial infarction (AMI).Materials and MethodsIn all, 114 AMI patients underwent cardiac MRI at 3T within 2–4 days (baseline) and at 6 months (follow‐up) after reperfusion. Late gadolinium enhancement and cine sequences were performed. The impact of MVO (ie, presence and extent) on regional wall thickening (WT, %), end‐diastolic wall thickness (EDWT, mm), and local cavity change (mm) of LV were quantitatively analyzed. Local cavity change, calculated as surface‐to‐surface distance from registered endocardial surface meshes of cine imaging datasets acquired initially and at follow‐up, was used to assess local remodeling.ResultsMVO was detected in 69 patients (60.5%). WT was significantly lower when MVO was present (P < 0.05); and it was inversely related to MVO transmural extent (P < 0.0001). WT improvement was significantly worsened when MVO was present in segments with infarct transmural extent exceeding 50%. Significant wall thinning occurred at follow‐up in segments with infarct transmural extent >75% with further thinning by MVO presence; and EDWT decreased with increasing MVO transmural extent (P < 0.0001). LV cavity shrank in patients without MVO, whereas it dilated in those with MVO. Local cavity changes were not significantly different by a region‐to‐region analysis throughout the LV within each group (P = 0.57 and 0.74, respectively).ConclusionMVO has a significant adverse effect on LV wall characteristics and LV remodeling. Postinfarct remodeling seems to be globally mediated rather than locally mediated during the first 6 months.Level of Evidence: 2Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2018;47:499–510.
Ex vivo MRI evaluation of prostate cancer: Localization and margin status prediction of prostate cancer in fresh radical prostatectomy specimens
Authors: Heidkamp Jan , Hoogenboom Martijn , Kovacs Iringo E. , Veltien Andor , Maat Arie , Sedelaar J.P. Michiel , Hulsbergen‐van de Kaa Christina A. , Fütterer Jurgen J. 
Published: June 2017

PurposeTo investigate the ability of high field ex vivo magnetic resonance imaging (MRI) to localize prostate cancer (PCa) and to predict the margin status in fresh radical prostatectomy (RP) specimens using histology as the reference standard.Materials and MethodsThis Institutional Review Board (IRB)‐approved study had written informed consent. Patients with biopsy‐proved PCa and a diagnostic multiparametric 3T MRI examination of the prostate prior to undergoing RP were prospectively included. A custom‐made container provided reference between the 7T ex vivo MRI obtained from fresh RP specimens and histological slicing. On ex vivo MRI, PCa was localized and the presence of positive surgical margins was determined in a double‐reading session. These findings were compared with histological findings obtained from completely cut, whole‐mount embedded, prostate specimens.ResultsIn 12 RP specimens, histopathology revealed 36 PCa lesions, of which 17 (47%) and 20 (56%) were correlated with the ex vivo MRI in the first and second reading session, respectively. Nine of 12 (75%) index lesions were localized in the first session, in the second 10 of 12 (83%). Seven and 8 lesions of 11 lesions with Gleason score >6 and >0.5 cc were localized in the first and second session, respectively. In the first session none of the four histologically positive surgical margins (sensitivity 0%) and 9 of 13 negative margins (specificity 69%) were detected. In second session the sensitivity and specificity were 25% and 88%, respectively.ConclusionEx vivo MRI enabled accurate localization of PCa in fresh RP specimens, and the technique provided information on the margin status with high specificity.Level of Evidence: 1Technical Efficacy: Stage 1J. Magn. Reson. Imaging 2018;47:439–448.
Assessment of climate change impacts on streamflow and hydropower potential in the headwater region of the Grande river basin, Southeastern Brazil
Authors: de Oliveira Vinícius A. , de Mello Carlos R. , Viola Marcelo R. , Srinivasan Raghavan 
Published: June 2017

ABSTRACTMaintaining water availability and electric energy production are the major concerns for the future in countries which are extremely dependent on their water resources like Brazil. The objective of this study was to assess the impacts of climate change on streamflow and therefore on hydropower potential of the Grande river basin headwater region, Southeast Brazil. For this purpose, the SWAT model was used to simulate the hydrological behaviour of the headwaters under the Representative Concentration Pathways 4.5 and 8.5 scenarios, obtained from the Regional Climate Models Eta‐HadGEM‐ES and Eta‐MIROC5 between the years 2007 and 2099. Through power duration curves we estimated the hypothetical average annual energy production at three hydropower plants installed in cascade in the region, being, from upstream to downstream, Camargos, Itutinga and Funil. These plants account for a potential of 277 MW. SWAT was able to simulate the streamflow of the Grande river basin headwaters and, therefore, to reproduce the observed monthly streamflow from the baseline period (1961–2005) reasonably well for all three hydropower plants. In general, the results indicated a significant streamflow reduction and therefore reductions in runoff during all the simulated periods and all radiative forcing analysed, when compared to the baseline period. Thus, these results led to reductions in hydropower potential and hence decreases of the annual energy production varying from 6.1 to 58.6% throughout the 21st Century.
Extreme point rainfall temporal scaling: a long term (1805–2014) regional and seasonal analysis in Spain
Authors: Gonzalez Sergi , Bech Joan 
Published: June 2017

ABSTRACTThis paper presents a regional and seasonal study of extreme point rainfall scaling from 10 min to 2 years. To do this, the highest point‐based rainfall list based on these temporal periods was calculated from the Spanish Meteorological Service (AEMET) precipitation databases with more than 11 000 rain‐gauge stations, with the longest series ranging from 1805 to 2014 (209 years). This list constitutes the register of single station largest amounts of precipitation in Spain ever recorded for selected periods, including for example the values for 2 h (193 mm), 24 h (817 mm) or 1 year (5503 mm). Rainfall extremes for 10 min periods are evenly distributed in coastal and inland areas. Daily precipitation extremes are mostly concentrated over the Mediterranean coast while from durations from one month to two years, extremes are located in southern and northwest Spain. Extreme data obtained were compared with existing worldwide rainfall records for equivalent periods. Results indicate that Spanish extreme rainfall scaling relating R depth (in mm) against D duration (in minutes) may be expressed as a potential law R = 21.8 D0.422 (R = 43.6 D0.507 for worldwide data). We propose the upper envelope line (greater or equal to extreme rainfall values) parallel to the potential fit law as a simple method to estimate possible extreme records for different time scales. Using this method, worldwide envelope may be expressed as R = 60.5 D0.507 and the Spanish envelope as R = 39.3 D0.422. Further analysis stratifying results by season and region show that seasonal scaling has more variability than regional scaling. The methodology described can be readily applied to other regions for which detailed rainfall databases exist. Applications of the results include using the scaling found as a reference for classification of heavy precipitation events for temporal scales.
Different roles of dynamic and thermodynamic effects in enhanced semi‐arid warming
Authors: Guo Ruixia , Guan Xiaodan , He Yongli , Gan Zewen , Jin Hongchun 
Published: June 2017

ABSTRACTEnhanced warming in semi‐arid regions has received much attention since it was first proposed, but the primary driver of this phenomenon remains unknown. This study applied dynamical adjustment to surface air temperature and partitioned the warming into two separate components: a thermodynamically forced component and a dynamically induced component. The results show that the mean amount of thermodynamic warming in the Northern Hemisphere in the study period 1902–2011 was 1.36 °C/109 years and that the amount of dynamic warming was 0.14 °C/109 years. In the mid‐latitude zones of Asia, Europe, and North America, the thermodynamic warming was 1.60, 1.19, and 1.32 °C/109 years, respectively, and the corresponding dynamic warming was 0.26, 0.14, and 0.09 °C/109 years. Obviously, higher thermodynamic temperature warming was observed in semi‐arid regions, suggesting that the enhanced semi‐arid warming (ESAW) is the result of local thermodynamic effects. Thus, different local thermodynamic effects are responsible for the warming discrepancies in the semi‐arid regions of Asia, Europe, and North America. Moreover, the considerable bias of Coupled Model Intercomparison Project Phase 5 (CMIP5) ensemble mean temperature trend appeared in semi‐arid regions rather than other regions indicate that the simulation of semi‐arid regions is particularly complex and difficult.
Adjuvant chemotherapy in resectable synovial sarcoma
Authors: Vining Charles C. , Sinnamon Andrew J. , Ecker Brett L. , Kelz Rachel R. , Fraker Dougas L. , Roses Robert E. , Karakousis Giorgos C. 
Published: June 2017

IntroductionThe role of adjuvant chemotherapy (AC) for synovial sarcoma (SS) is controversial. Using a large national dataset we evaluate the impact of AC on overall survival (OS) following curative‐intent resection of SS.MethodsPatients with stage I‐III SS 2004‐2012 undergoing resection were identified in the National Cancer Data Base. Clinicopathologic factors associated with OS were identified using Cox proportional‐hazard modeling, adjusting for factors associated with receipt of AC.ResultsAmong 544 patients, 131 received AC. Median age of the cohort was 42 years; 269 were female. AC was not associated with OS in univariate analysis in the overall cohort. After stratification by stage, AC was associated with prolonged OS in univariate analysis in stage III patients only (P = 0.028), which remained significant in multivariable analysis (P = 0.033). Other factors associated with OS in stage III patients were age >55 years, (P = 0.001), positive margins (P = 0.037), biphasic histology (P = 0.013), and adjuvant radiation (P < 0.001). The association between AC and OS remained significant after adjustment for factors predictive of receipt of AC.ConclusionsIn this national cohort, AC was associated with prolonged OS for SS for stage III patients but not in lower stages. Less restricted use of this therapy may be warranted in this population.