bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Protective Efficacy of Selected Immunodominant B-Cell Epitopes within Virulent Surface Proteins of Streptococcus pneumoniae
Authors: Papastamatiou Theodora , Routsias John G. , Koutsoni Olga , Dotsika Eleni , Tsakris Athanassios , Spoulou Vana 
Published: December 2017

ABSTRACT

Four previously identified immunodominant B-cell epitopes, located within known virulent pneumococcal proteins CbpD, PhtD, PhtE, and ZmpB, had shown promising
in vivo
immunological characteristics, indicating their potential to be used as vaccine antigens. In this study, we further evaluated the opsonophagocytic activity of antibodies against these epitopes and their capacity to protect mice from pneumococcal sepsis. An opsonophagocytic killing assay (OPKA) revealed that OPKA titers of human anti-peptide antibodies against pneumococcal serotypes 1, 3, and 19A were significantly higher (
P
< 0.001) than those of the control sera, suggesting their functional potential against virulent clinical isolates. Data obtained from mice actively immunized with any of the selected epitope analogues or with a mixture of these (G_Mix group) showed, compared to controls, enhanced survival against the highly virulent pneumococcal serotype 3 (
P
< 0.001). Moreover, passive transfer of hyperimmune serum from G_Mix to naive mice also conferred protection to a lethal challenge with serotype 3, which demonstrates that the observed protection was antibody mediated. All immunized murine groups elicited gradually higher antibody titers and avidity, suggesting a maturation of immune response over time. Among the tested peptides, PhD_pep19 and PhtE_pep40 peptides, which reside within the zinc-binding domains of PhtD and PhtE proteins, exhibited superior immunological characteristics. Recently it has been shown that zinc uptake is of high importance for the virulence of
Streptococcus pneumoniae
; thus, our findings suggest that these epitopes deserve further evaluation as novel immunoreactive components for the development of a polysaccharide-independent pneumococcal vaccine.
Phosphocreatine pathway for energy transport: ADP diffusion and cardiomyopathy
Authors: Saks V. A. , Belikova Y. O. , Kuznetsov A. V. , Khuchua Z. A. , Branishte T. H. , Semenovsky M. L. , Naumov V. G. 
Published: December 2017

Chemically skinned (by treatment with saponin, 40 μg/ml) isolated cardiomyocytes were used to study the intracellular diffusion of ADP and creatine (Cr). Stimulation of respiration was studied in these cardiomyocytes without intact sarcolemma and in isolated heart mitochondrial by addition of ADP and Cr in the presence of 0.2 mM ATP (via mitochondrial creatine kinase reaction: Cr + MgATP = MgADP + PCr). The Michaelis constant (Km) for Cr was similar in both cases, 5.67 ± 0.11 (SD) mM in skinned myocytes and 6.9 ± 0.2 mM in mitochondria, showing that there is no significant restriction to the diffusion of this substrate. However, the apparent Km for external ADP increased from 17.6 ± 1.0 μM for mitochondria to 250 ± 38 μM for skinned cardiomyocytes, showing decreased diffusivity of ADP as a result of binding to cellular structures. In the presence of 25 mM Cr, the Km for ADP for myocytes decreased to 35.6 ± 5.6 μM due to the coupling of the creatine kinase and oxidative phosphorylation reactions. Provision of substrate for the creatine kinase reaction amplified the weak ADP signal in the regulation of respiration. The activity of the mitochondrial creatine kinase was decreased by a factor of two in cardiomyopathic hamsters and human hearts and was associated with a twofold decrease in creatine-stimulated respiration. These data show a potentially key role of mitochondrial creatine kinase in the regulation of cellular respiration and the possible importance of changes in its activity for the functional disturbances of the cardiomyopathic heart. cellular respiration; creatine kinase; oxidative phosphorylation
Differences and similarities in albumin and red blood cell flows through cerebral microvessels
Authors: Tajima A. , Nakata H. , Lin S. -Z. , Acuff V. , Fenstermacher J. 
Published: December 2017

Pages H1515–H1524: A. Tajima, H. Nakata, S.-Z. Lin, V. Acuff, and J. Fenstermacher. “Differences and similarities in albumin and red blood cell flows through cerebral microvessels.” Page H1521: right column, second paragraph, the first four lines read as follows: If the cycling period is <10 s for the system, then all of the capillaries will be perfused and labeled within 10 s of the first appearance of RISA and radiotagged RBCs in cerebral capillaries. The time from intravenous injection to radiotracer appearance in cerebral capillaries is ap7 s in the awake rat.
PAR4 (Protease-Activated Receptor 4) Antagonism With BMS-986120 Inhibits Human Ex Vivo Thrombus Formation
Authors: Wilson Simon J. , Ismat Fraz A. , Wang Zhaoqing , Cerra Michael , Narayan Hafid , Raftis Jennifer , Gray Timothy J. , Connell Shea , Garonzik Samira , Ma Xuewen , Yang Jing , Newby David E. 
Published: December 2017

Objective—
BMS-986120 is a novel first-in-class oral PAR4 (protease-activated receptor 4) antagonist with potent and selective antiplatelet effects. We sought to determine for the first time, the effect of BMS-986120 on human ex vivo thrombus formation.

Approach and Results—

Forty healthy volunteers completed a phase 1 parallel-group PROBE trial (Prospective Randomized Open-Label Blinded End Point). Ex vivo platelet activation, platelet aggregation, and thrombus formation were measured at 0, 2, and 24 hours after (1) oral BMS-986120 (60 mg) or (2) oral aspirin (600 mg) followed at 18 hours with oral aspirin (600 mg) and oral clopidogrel (600 mg). BMS-986120 demonstrated highly selective and reversible inhibition of PAR4 agonist peptide (100 μM)-stimulated P-selectin expression, platelet-monocyte aggregates, and platelet aggregation (
P
<0.001 for all). Compared with pretreatment, total thrombus area (μm
2
/mm) at high shear was reduced by 29.2% (95% confidence interval, 18.3%–38.7%;
P
<0.001) at 2 hours and by 21.4% (9.3%–32.0%;
P
=0.002) at 24 hours. Reductions in thrombus formation were driven by a decrease in platelet-rich thrombus deposition: 34.8% (19.3%–47.3%;
P
<0.001) at 2 hours and 23.3% (5.1%–38.0%;
P
=0.016) at 24 hours. In contrast to aspirin alone, or in combination with clopidogrel, BMS-986120 had no effect on thrombus formation at low shear (
P
=nonsignificant). BMS-986120 administration was not associated with an increase in coagulation times or serious adverse events.

Conclusions—
BMS-986120 is a highly selective and reversible oral PAR4 antagonist that substantially reduces platelet-rich thrombus formation under conditions of high shear stress. Our results suggest PAR4 antagonism has major potential as a therapeutic antiplatelet strategy.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT02439190.
The neural events that change perception
Authors: Krug Kristine , Parker Andrew J. 
Published: December 2017

Abstract
Neuroscientific research has made tremendous progress towards unravelling the neuronal codes that underlie our rich sensory perception and experience. From single neurons in primates’ visual brain that predict perceptual choices to activity patterns in defined neuronal circuits, electrical activity across different levels correlates with perception. The key to how neuronal signals give rise to our visual experience lies in causal interventions directly applied to neurons and circuits, interventions that alter perception naturalistically and in predictable ways. The most powerful and reliable intervention method in primates remains invasive electrical micro-stimulation, which can change selectively the appearance of visual objects defined by more than one visual cue. Such artificial signals are integrated with visually evoked stimuli and with contextual factors like reward. Scaling up these methodologies presents opportunities for vision replacement through cortical neuro-prosthetics.
Effect of Urea on the Shape and Structure of Carbon Nanotubes
Authors: Elamin M. R. , Abdulkhair Babiker Y. , Taha Kamal K. 
Published: December 2017

Abstract
Coiled multiwall carbon nanotubes (MWCNTs) were prepared on Fe, Co, and Ni metal oxides supported on α-Al2O3 using urea as fuel and catalyst surface modifying agent by catalytic chemical vapour deposition (CCVD). The shape of the nanotubes was influenced by the addition of urea, where coiled and uncoiled tubes were obtained in the presence and absence of urea, respectively. The MWCNTs were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and nitrogen adsorption analysis. The coiling/uncoiling of the nanotubes was visualized from the SEM and TEM images of the prepared specimens. The XRD data showed the characteristic peaks of the nanotubes. BET analysis of the coiled tubes revealed 85.57 m2 g−1 surface area with a pore diameter 102.2–110.8 Å. A mechanism for the nanotubes coiling is suggested.
Bienestar psicológico e involucramiento religioso en estudiantes universitarios de Lima
Authors: Escudero Nolasco Juan Carlos 
Published: December 2017

El objetivo del estudio fue establecer la relación entre el bienestar psicológico y el involucramiento religioso, así como, encontrar diferencias en el bienestar psicológico entre las distintas formas de involucramiento religioso. La muestra estuvo constituida por 297 estudiantes universitarios de la facultad de psicología de una universidad pública de Lima Metropolitana. Para la recolección de datos se utilizó la Escala de bienestar psicológico para adultos (BIEPS) adaptada al contexto peruano y un cuestionario sobre involucramiento religioso elaborado por el autor. Los resultados evidenciaron que no existe relación entre el bienestar psicológico y el involucramiento religioso, sin embargo, se encontró que el grupo de alto involucramiento religioso que mantiene participación activa en grupos religiosos presenta mayor bienestar psicológico general y en el componente proyectos de forma significativa, en comparación a los que mantienen otras formas de involucramiento religioso.
Gospodarka współdzielenia – pojęcie, źródła, potencjał
Authors: Sztokfisz Barbara 
Published: December 2017

The paper presents a theoretical overview of definitions of the sharing economy. It emphasises the role of the access to goods and services, which are systematically taking the place of ownership. It refers to the main categorizations presented in the literature as well as the genesis of the concept. It presents the sharing economy as an alternative model of consumption, one which seeks to increase the efficiency of the resources creating new value for societies. This new value is largely possible through the digital revolution providing previously inaccessible tools, through new quality in interpersonal relationships as well as increasing social awareness. The aim of the paper is to provide a systematic review of the literature and to clarify the principle of true sharing as opposed to a profit-based orientation.
Optimization of family planning service in Ukraine in the realization of modern strategy of woman health maintenance
Authors: Ю. Г. Антипкін  , В. В. Камінський  , Т. Ф. Татарчук  
Published: December 2017

The primary objective of the reproductive health services system and its individual institutions are, namely: to implement the strategy of women management starting from their fetal life to the old age and ensure quality of life in adolescence, reproductive and postmenopausal periods. Maternal and child death rates are above respectives rates in European countries, and utilization rate of modern contraceptive methods in Ukraine remains low. This can be explained by both, poor awareness of women and insufficient training of medical personnel. In this context, the top priority still is awareness-raising and further implementation of the strategy on reliable contraception means and methods along with effective preconception care as a potent component of family planning.Taking into account modern global trends, the principal goal of Family Planning Service in Ukraine is to improve women’s quality of life and time of active ageing, which achievement can be facilitated by the implementation of 3 priority tasks:• continued realization of the strategy on reliable contraception use and implementation of effective preconception care to abortions prevention and maternal and newborns mortality rates reduction;• reduction cardiovascular and oncological diseases incidence rate in women through development and implementation of respective screening programs, informational and preventive measures;• implementation of best international practices with respect to Family Planning Service efficacy, review and update of family planning clinical protocol with further implementation of such practices at the national and regional levels.Considering this, the Resolution of Advisory Board outlines the main tasks for the implementation of modern international experience and its further implementation at the national level, and amendments to clinical protocol of primary, secondary (specialized) and tertiary (highly specialized) medical care (approved by the order of MOH of Ukraine No. 59 dated January 21, 2014) “Family Planning”. The main additions and comments touched upon the issues of micronutrient status and reproductive health of women, contraceptive methods for women in perimenopause, counseling and selection of contraceptive methods for HIV-positive women. Particular attention was paid to counseling and selection of contraceptive methods for women military personnel.
O LSD DENTRO DA BUSCA ESPIRITUAL DE FAUZI ARAP
Authors: DELMANTO JÚLIO 
Published: December 2017

THE ROLE OF LSD WITHIN FAUZI ARAP'S SPIRITUAL QUEST EL LSD DENTRO DE LA BÚSQUEDA ESPIRITUAL DE FAUZI ARAP LE LSD DANS LA QUáŠTE SPIRITUELLE DE FAUZI ARAP
Actualización en Miastenia gravis.
Authors: Castro-Suarez Sheila , Caparó-Zamalloa César , Meza-Vega María 
Published: December 2017

La miastenia gravis (MG) es una enfermedad autoinmune, caracterizada por fatiga y debilidad muscular localizada o generalizada, a predominio proximal y de curso fluctuante; los síntomas provienen del bloqueo post-sináptico de la transmisión neuromuscular por anticuerpos contra los receptores de acetilcolina y otras proteínas de la membrana post-sináptica. La incidencia es de 8 a 10 casos por millón de personas y la prevalencia, de 150 a 250 por millón; estas cifras, sin embargo, varían en las diferentes poblaciones estudiadas. El diagnóstico de MGse basa en el cuadro clínico y resultados positivos de tests tales como anticuerpos específicos, test neurofisiológicos o prueba terapéutica. La búsqueda de patologías asociadas es un paso importante en la evaluación. El tratamiento se sustenta en tres pilares: tratamiento con fármacos inhibidores de la acetilcolinesterasa (piridostigmina), inmunoterapia (corticoides o inmunosupresores/inmunomoduladores) e intervención quirúrgica (timectomia).
Desenvolvimento sustentável e algumas vulnerabilidades geradas pelo não atendimento de necessidades básicas
Authors: De Assis Wilma , Elisa Maria 
Published: December 2017

O tema deste artigo se insere no contexto atual de transição,ou de tentativas de transição, de um modelo de desenvolvimentoconsiderado insustentável para outro, sustentável. Analisa essatransição pela presença de organizações internacionais atuandoem prol da humanização do desenvolvimento e pela existência denovos indicadores de desenvolvimento. Tem por objetivo abordar asvulnerabilidades individuais/sociais possíveis de serem geradas pelonão atendimento de necessidades humanas. Utiliza como métodoa sobreposição dos conceitos de desenvolvimento, necessidades evulnerabilidades, recorrendo a algumas disposições metodológicas dateoria da complexidade. Conclui sobre a importância de abordagenseconômicas aproximarem-se da complexidade, utilizarem os novos indicadores de sustentabilidade e de valores humanos. Consideraa proposição de que, quando a economia muda, o desenvolvimentomuda. Quando o ser humano muda, a economia e o desenvolvimentotambém mudam.
Analysis on Flexural Stiffness of GFRP-concrete Hybrid Beams
Authors: Zhao Fei , Chen Chaohe , Guo Haihao 
Published: December 2017

Background:
Deferent types of reinforced concrete beams wrapped with GFRP pultruded profiles and a control RC beam are tested under three points loading.

Objective:
The moment-curvature curves of all tested beams are obtained based on the experimental study. The change characteristics of flexural stiffness for tested beams are analyzed, and the short-term flexural stiffness formula is derived.

Results:
Finally the computed values of midspan deflection for tested beams are achieved.
Research on Base Isolation for Seismic Retrofitting of Adjacent Structures Connected with a Large Floor
Authors: Chen Kai , Miao Zhiwei , Li Aiqun 
Published: December 2017

Introduction:
Seismic isolation has been proven to be an efficient approach to seismic retrofitting of structures based on the concept of reducing the seismic forces by lengthening the structural fundamental vibration period. However, superstructures may collide with each other under seismic excitation as the top displacement increases in the case of base-isolated adjacent structures. The relative displacement of top floors of superstructures is decreased more effectively by connecting the isolation layer of adjacent structures with a large floor, compared with the method to increase the width of seismic joint by using building monolithic movement technology. A specific engineering of base isolation for retrofitting of existing adjacent frame structures is studied in this paper.

Methods:
Seismic response was evaluated for the structures in different cases by time-history analysis. Parametric studies are performed in order to achieve the laws of top displacement difference of superstructures influenced by the change of dynamic characteristic of towers in the case of base-isolated multi-tower structure with a large floor.

Results and Conclusion:
The results of extensive numerical analysis verify the effectiveness of isolation with a large floor in minimizing the forces from earthquake and protecting the top story from crashing into each other, which has important reference value for application of retrofitting with isolation technology on adjacent reinforced concreted frames.
Resumos de tese e dissertação
Authors: lnês Parellada Claudia , Zanettini  Paulo , Rambelli Gilson , Gomide da Silva Mello Márcia , Canlo Lopes Paulo Roberto do 
Published: December 2017

Um tesouro herdado: os vestígios arqueológicos da cidade colonial espanhola de Villa Rica Del Espiritu Santo/Fênix (PR)
Claudia Inês Parellada

A Calçada do Lorena: um caminho para o mar
Paulo Zanettini

A Arqueologia Subaquática e sua aplicação à Arqueologia Brasileira: o exemplo do baixo vale do Ribeira de Iguape
Gilson Rambelli

Sistematizações de critérios para diagnóstico diferencial entre paleopatologias e sinais de alterações análogas: fundamentos teórico-metodológicos
Márcia Gomide da Silva Mello

A colonização portuguesa na Ilha de Marajó: espaço e contexto arqueológico-histórico na Missão Religiosa de Joanes
Paulo Roberto do Canlo Lopes
Oscilação climática observada nas camadas de voçorocas do sudoeste de Goiás
Authors: Ribeiro Maira Barberi , Schmitz Pedro lgnacio 
Published: December 2017

Depois da seqüêncía de maior e menor unidade obseruada no abrigo GO-JA-Ì|, datada entre aproxima damente 10,500 e 6.000 A.p., obseruamos e descrevemos uma seqüêncía geológica encontrada numa voçoroca da mesma localidade (GO-JA-23), para a qual foram conseguidas 7 datações, que vão de 4.505 a 2.140 A.p. Buscamos interpretar os estratos geológicos em termos de paleo-clima.
UKURAN TOKO DAN HOMOGENITAS BARANG DAGANGAN PENGARUHNYA TERHADAP DAYA TARIK TOKO TERHADAP KONSUMEN
Authors: Mulia Dipa 
Published: December 2017

Munculnya toko toko baru biasanya menimbulkan “pergerakan konsumen” dari satu toko yang sudah ada kepada toko yang baru muncul. Toko toko yang letaknya saling berdekatan saling mempengaruhi satu sama lain dalam memperebutkan konsumen yang sama sehingga dapat dikatakan bahwa kinerja toko secara keseluruhan menjadi tidak efisisien. Model Elektrostatik atau Model Coulomb yang diperkenalkan oleh ahli Fiska abad 18, Charles Augustine de Coulomb dapat digunakan dalam menganalisis pengaruh toko terhadap konsumennya sehingga dapat dibuatkan desain toko agar kinerjanya menjadi efisien. Makalah ini dibuat melalui riset diskriptif dengan menggunakan pendekatan kuantitatif dengan mengambil sampel 5 buah toko dengan ukuran dan jenis yang berbeda. Responden diambil sebanyak 60 orang per toko dengan total responden sebanyak 300 orang. Hasil penelitian menunjukkan bahwa besar ukuran toko dan keragaman produk yang dijual mempengaruhi daya tarik toko terhadap konsumen.Introducing new store to customer usually create the “customer shift” from the existing store to the new one. When the new store is located nearby the existing one, the shifting could not be avoided; it means the efficiency of store is becoming less than before. In order to optimize the efficiency of store the distance between stores should be managed. This paper implements theClassic Model of Electrostatic Forces that also known as Model of Coulomb.The Model was promoted by France Physicist, Charles Augustine de Coulomb, in 18th Century. The attractiveness of store to customer is represented by the electrostatic force on the Model. This paper is a descriptive research with a quantitative approach; the object of research is 5 (five) stores with different types in term of size and product offered. There were 60 qualified respondents selected on each store, in total it was 300 respondents. The result of the research tells that the size of store and the homogeneity of product offered are influencing the attractiveness ofthe store to the customer.
APLIKASI REGRESI LOGISTIK DALAM ANALISIS FAKTOR RISIKO ANEMIA GIZI PADA MAHASISWA BARU IPB
Authors: Maharani Indah Irma , Hardinsyah Hardinsyah , Sumantri Bambang 
Published: December 2017

<p class="MsoNormal" style="margin: 0cm 12.6pt 6pt 18pt; text-align: justify; text-indent: 27pt;"><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us">This study was aimed at analyzing risk factors of nutritional anemia among new students of Bogor Agricultural University (IPB). A logistic regression model for 1907 new students’ record data matched with health record data was selected and applied on anemia risk factors analysis. The result showed that 10.9% and 21.4% of the students were suffering from nutritional anemia and proteinuria respectively. The risk factors of nutritional anemia among the new students of IPB are sex, age, family income and proteinuria status. The nutritional anemia risk is higher among female students (OR = 1.36); among younger age (age &lt; 18 year); among students from low income family (OR = 1.70); and students suffered from proteinuria (OR = 1.49). This implies that the regular monitoring and controlling of both anemia and proteinuria among the students by Polyclinic of IPB are urgently required. </span></p>