bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrocardiographic Responses to Deer Hunting in Men and Women
Authors: Verba Steven D. , Jensen Brock T. , Lynn Jeffrey S. 
Published: July 2016

Objective Deer hunting includes various stimuli resulting in augmented sympathetic activity, increased heart rate (HR) response, and rhythm changes. Collectively, these superimposed stresses may increase an individual’s risk for cardiovascular events. We undertook this study to evaluate HR and rhythm responses in multiple phases of deer hunting in men and women with and without cardiovascular disease (CVD). Methods Nineteen participants age 38.3 ± 13.8 years (mean ± SD) with body mass index 29.2 ± 6.9 kg/m2 followed their normal hunting routine. HR and rhythm were recorded continuously during the hunt using a small leadless electrocardiogram (ECG) patch monitor. Results Data were collected on 13 of 19 hunters while hiking. Three hunters recorded HR ≥85% of their age-predicted heart rate maximum (HRmax) for 1 to 2 minutes. Arrhythmias were detected in both participants with CVD and in 8 without CVD. Recorded rhythms included premature atrial, junctional, and ventricular complexes. Six hunters climbed a tree stand; 3 of them recorded HR ≥85% HRmax with sustained elevated HR response for 2 to 3 minutes with premature junctional contractions. Four of 19 participants dragged deer carcasses. During the drag, 1 male hunter recorded an HR of 91% HRmax, and another male hunter without CVD recorded an exercise-induced ischemic ECG. Fifteen of 19 hunters experienced “buck fever” (acute extreme excitation), with 7 reaching ≥85% HRmax for up to 1 minute. Ventricular bigeminy and trigeminy and ventricular couplets were observed in 1 subject during buck fever. Conclusions Men and women with and without CVD recorded substantial increases in HR and clinically relevant arrhythmias while deer hunting.
Epidemiology of Feature-Specific Injuries Sustained by Skiers in a Snow Park
Authors: Carús Luis , Escorihuela María 
Published: July 2016

Objective The objective of the present case series study was to analyze injury types and injured anatomic locations resulting from skiing in snow park (SP) features and to determine potential risk factors for ski injuries in an SP. Methods The study was conducted during the 2013–2014 winter season in the SP of a major winter resort located in the Spanish Pyrenees. Cases involved skiers who experienced feature-related injuries in the SP. A total of 113 cases met the inclusion criteria. Logistic regression was used to calculate the odds of injury types and injury to anatomic locations on aerial versus nonaerial features. Results The overall injury rate was 0.9 per 1000 skier runs. The proportion of injuries was higher for aerials (1.18% of uses) than for nonaerials (0.66% of uses). Results revealed that the upper extremities were the most commonly injured body region, and sprains/strains/dislocations and fractures were the most common injury type. Conclusions The most commonly injured anatomic location on nonaerial features was the face, while on aerial features it was the head. A higher proportion of fractures was observed on aerial features, while a higher proportion of sprains/strains/dislocations was observed on nonaerial features. Prevention strategies to reduce injury risk include SP redesign, safety and communication policies, instruction on technical skills, and promotion of the use of protective equipment.
Reliability modelling for rotorcraft component fatigue life prediction with assumed usage
Authors: Dekker S. , Wurzel G. , Alderliesten R. 
Published: July 2016

ABSTRACTFatigue life is a random variable. Thus, the reliability of a conservative fatigue life prediction for a component in the helicopter dynamic system needs to be substantiated. A standard analytical substantiation method uses averaged manoeuvre loads instead of seeing manoeuvre loads as a random variable whose distribution is estimated with limited precision. This simplification may lead to inaccuracies. A new simulation-based method is developed to conservatively predict fatigue life, while also accounting for the full random distribution and uncertainty of manoeuvre loads. Both methods fully account for uncertain fatigue strength but assume that the mission profile is known or can at least be conservatively estimated. Simulations under synthetic but realistic engineering conditions demonstrate that both methods may be used for accurate substantiation of conservative fatigue life predictions. The simulations also demonstrate that, under the tested conditions, uncertainties from manoeuvre loads may be neglected in fatigue life substantiations as the resulting error is not significant with respect to uncertainties in component fatigue strength.
Periodic controllers for vibration reduction using actively twisted blades
Authors: Brillante Claudio , Morandini Marco , Mantegazza Paolo 
Published: July 2016

ABSTRACTThis paper compares two periodic control methods, the optimalH2and the periodic static output feedback (POF), to reduce the helicopter rotor vibrations. Actively twisted blades with Macro-Fibre Composite (MFC) piezoelectric actuators are used. The design model is based on a simplified aerodynamic model and on a multi-body model of the Bo 105 isolated rotor with the original blades replaced by actively twisted ones. The performance of the two controllers in alleviating hub loads is verified with improved simulations based on a free-wake model.
A comparison of undergraduate teaching of psychiatry across medical schools in the Republic of Ireland
Authors: Byrne F. , Murphy R. , O’Rourke L. , Cotter D. , Murphy K. C. , Guerandel A. , Meagher D. , Sweeney E. , Gill M. , Campbell A. , McDonald C. , Hallahan B. 
Published: July 2016

ObjectivesTo examine the delivery and assessment of psychiatry at undergraduate level in the six medical schools in the Republic of Ireland offering a medical degree programme.MethodsA narrative description of the delivery and assessment of psychiatry at undergraduate level by collaborative senior faculty members from all six universities in Ireland.ResultsPsychiatry is integrated to varying degrees across all medical schools. Clinical experience in general adult psychiatry and sub-specialities is provided by each medical school; however, the duration of clinical attachment varies, and the provision of some sub-specialities (i.e. forensic psychiatry) is dependent on locally available resources. Five medical schools provide ‘live’ large group teaching sessions (lectures), and all medical schools provide an array of small group teaching sessions. Continuous assessment encompasses 10–35% of the total assessment marks, depending on the medical school. Only one medical school does not provide a clinical examination in the form of an Objective Structured Clinical Examination with viva examinations occurring at three medical schools.ConclusionsMany similarities exist in relation to the delivery of psychiatry at undergraduate level in Ireland. Significant variability exists in relation to assessment with differences in continuous assessment, written and clinical exams and the use of vivas noted. The use of e-learning platforms has increased significantly in recent years, with their role envisaged to include cross-disciplinary teaching sessions and analysis of examinations and individual components within examinations which will help refine future examinations and enable greater sharing of resources between medical schools.
Serotonin and poor neonatal adaptation after antidepressant exposurein utero
Authors: Kieviet Noera , van Keulen Vera , van de Ven Peter Marinus , Dolman Koert Melchior , Deckers Martine , Honig Adriaan 
Published: July 2016

ObjectiveInfants exposed to selective antidepressants (SADs)in uteroare at risk to develop poor neonatal adaptation (PNA)postpartum. As symptoms are non-specific and the aetiology of PNA is unknown, the diagnostic process is hampered. We hypothesised that the serotonin metabolism plays a role in the aetiology of PNA.MethodsIn this controlled study, infants admittedpostpartumfrom February 2012 to August 2013 were included and followed for 3 days. Infants exposed to SADs during at least the last 2 weeks of fetal life were included in the patient group (n=63). Infants not exposed to psychotropic medication and admittedpostpartumfor another reason were included in the control group (n=126). The neonatal urinary 5-hydroxyindoleacetid acid (5-HIAA) levels of SAD-exposed infants who developed PNA, SAD-exposed infants who did not develop PNA and control infants were compared.ResultsThe course of the 5-HIAA levels over the first 3 dayspostpartumdiffered between infants with and without PNA (p≤0.001) with higher 5-HIAA levels in infants with PNA on day 1 (2.42 mmol/mol,p=0.001). Presence of maternal psychological distress modified this relationship.ConclusionsA transient disturbance of the neonatal serotonergic system may play a role in the aetiology of PNA. Other factors, including the presence of maternal psychological distress, also seem to play a role.
THE LAW APPLICABLE TO THE ARBITRATION AGREEMENT: TOWARDS TRANSNATIONAL PRINCIPLES
Authors: Nazzini Renato 
Published: July 2016

AbstractThis article examines the problem of the law governing the validity of the arbitration agreement. The cases ofSulaméricain the English Court of Appeal and ofFirstLinkin the High Court of Singapore demonstrate that leading arbitration jurisdictions around the world can come to diametrically opposite results. In particular, there are currently diverging views as to whether the law applicable to the arbitration agreement should be the law chosen by the parties to govern their substantive legal relationship or the law of the seat of the arbitration. The issue is unlikely to be settled soon at international level. However, without embracing extreme approaches that purport to determine the validity of the arbitration agreement without reference to any national legal system, a more ‘transnational’ approach should be encouraged. This may emerge, based on three structured principles which would be desirable for international convergence, namely the non-discrimination principle, the estoppel principle and the validation principle. These principles can be developed without conflicting with the conventional conflicts-of-laws approach which was adopted by the English Court of Appeal inSulamérica.
Application of network analysis to identify interactive systems of eating disorder psychopathology
Authors: Forbush K. T. , Siew C. S. Q. , Vitevitch M. S. 
Published: July 2016

BackgroundTraditional approaches for the classification of eating disorders (EDs) attribute symptoms to an underlying, latent disease entity. The network approach is an alternative model in which mental disorders are represented as networks of interacting, self-reinforcing symptoms. This project was the first to use network analysis to identify interconnected systems of ED symptoms.MethodAdult participants (n = 143; 77.6% women) with a Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5) ED were recruited from the community to take part in a larger ongoing longitudinal study. The Structured Clinical Interview for DSM Disorders (SCID-I) was used to establish diagnoses. An undirected network of ED symptoms was created using items from the Eating Pathology Symptoms Inventory (EPSI) and the R package qgraph.ResultsBody checking emerged as the strongest and most important single symptom in the entire network by having the shortest average distance to other symptoms in the network, and by being the most frequent symptom on the path between any two other symptoms. Feeling the need to exercise every day and two symptoms assessing dietary restraint/restricting emerged as ‘key players’, such that their removal from the network resulted in maximal fracturing of the network into smaller components.ConclusionsAlthough cognitive–behavioral therapy for EDs focuses on reducing body checking to promote recovery, our data indicate that amplified efforts to address body checking may produce stronger (and more enduring) effects. Finally, results of the ‘key players analysis’ suggested that targeting interventions at these key nodes might prevent or slow the cascade of symptoms through the ‘network’ of ED psychopathology.
Timing of adding blood to prime affects inflammatory response to neonatal cardiopulmonary bypass
Authors: Schmidt Benjamin S. , Jordan James E. , Lane Magan R. , DiPasquale Vanessa M. , Graf Lori P. , Ootaki Yoshio , Ungerleider Ross M. 
Published: July 2016

AbstractComplications from systemic inflammation are reported in neonates following exposure to cardiopulmonary bypass. Although the use of asanguinous primes can reduce these complications, in neonates, this can result in significant haemodilution, requiring addition of blood. This study investigates whether the addition of blood after institution of bypass alters the inflammatory response compared with a blood prime. Neonatal swine were randomised into four groups: blood prime, blood after bypass but before cooling, blood after cooling but before low flow, and blood after re-warming. All groups were placed on central bypass, cooled, underwent low flow, and then re-warmed for a total bypass time of 2 hours. Although haematocrit values between groups varied throughout bypass, all groups ended with a similar value. Although they spent time with a lower haematocrit, asanguinous prime groups did not have elevated lactate levels at the end of bypass compared with blood prime. Asanguinous primes released less tumour necrosis factor α than blood primes (p=0.023). Asanguinous primes with blood added on bypass produced less interleukin 10 and tumour necrosis factor α (p=0.006, 0.019). Animals receiving blood while cool also showed less interleukin 10 and tumour necrosis factor α production than those that received blood warm (p=0.026, 0.033). Asanguinous primes exhibited less oedema than blood primes, with the least body weight gain noted in the end cool group (p=0.011). This study suggests that using an asanguinous prime for neonates being cooled to deep hypothermia is practical, and the later addition of blood reduces inflammation.
Differential specificity between closely related corals and abundant Endozoicomonas endosymbionts across global scales
Authors: Neave Matthew J , Rachmawati Rita , Xun Liping , Michell Craig T , Bourne David G , Apprill Amy , Voolstra Christian R 
Published: July 2016

Abstract
Reef-building corals are well regarded not only for their obligate association with endosymbiotic algae, but also with prokaryotic symbionts, the specificity of which remains elusive. To identify the central microbial symbionts of corals, their specificity across species and conservation over geographic regions, we sequenced partial SSU ribosomal RNA genes of Bacteria and Archaea from the common corals Stylophora pistillata and Pocillopora verrucosa across 28 reefs within seven major geographical regions. We demonstrate that both corals harbor Endozoicomonas bacteria as their prevalent symbiont. Importantly, catalyzed reporter deposition–fluorescence in situ hybridization (CARD–FISH) with Endozoicomonas-specific probes confirmed their residence as large aggregations deep within coral tissues. Using fine-scale genotyping techniques and single-cell genomics, we demonstrate that P. verrucosa harbors the same Endozoicomonas, whereas S. pistillata associates with geographically distinct genotypes. This specificity may be shaped by the different reproductive strategies of the hosts, potentially uncovering a pattern of symbiont selection that is linked to life history. Spawning corals such as P. verrucosa acquire prokaryotes from the environment. In contrast, brooding corals such as S. pistillata release symbiont-packed planula larvae, which may explain a strong regional signature in their microbiome. Our work contributes to the factors underlying microbiome specificity and adds detail to coral holobiont functioning.
Disruption of Kcc2-dependent inhibition of olfactory bulb output neurons suggests its importance in odour discrimination
Authors: Gödde Kathrin , Gschwend Olivier , Puchkov Dmytro , Pfeffer Carsten K. , Carleton Alan , Jentsch Thomas J. 
Published: July 2016

AbstractSynaptic inhibition in the olfactory bulb (OB), the first relay station of olfactory information, is believed to be important for odour discrimination. We interfered with GABAergic inhibition of mitral and tufted cells (M/T cells), the principal neurons of the OB, by disrupting their potassium-chloride cotransporter 2 (Kcc2). Roughly, 70% of mice died around 3 weeks, but surviving mice appeared normal. In these mice, the resulting increase in the intracellular Cl− concentration nearly abolished GABA-induced hyperpolarization of mitral cells (MCs) and unexpectedly increased the number of perisomatic synapses on MCs. In vivo analysis of odorant-induced OB electrical activity revealed increased M/T cell firing rate, altered phasing of action potentials in the breath cycle and disrupted separation of odour-induced M/T cell activity patterns. Mice also demonstrated a severely impaired ability to discriminate chemically similar odorants or odorant mixtures. Our work suggests that precisely tuned GABAergic inhibition onto M/T cells is crucial for M/T cell spike pattern separation needed to distinguish closely similar odours.
Integrative functional genomics identifies regulatory mechanisms at coronary artery disease loci
Authors: Miller Clint L. , Pjanic Milos , Wang Ting , Nguyen Trieu , Cohain Ariella , Lee Jonathan D. , Perisic Ljubica , Hedin Ulf , Kundu Ramendra K. , Majmudar Deshna , Kim Juyong B. , Wang Oliver , Betsholtz Christer , Ruusalepp Arno , Franzén Oscar , Assimes Themistocles L. , Montgomery Stephen B. , Schadt Eric E. , Björkegren Johan L.M. , Quertermous Thomas 
Published: July 2016

AbstractCoronary artery disease (CAD) is the leading cause of mortality and morbidity, driven by both genetic and environmental risk factors. Meta-analyses of genome-wide association studies have identified >150 loci associated with CAD and myocardial infarction susceptibility in humans. A majority of these variants reside in non-coding regions and are co-inherited with hundreds of candidate regulatory variants, presenting a challenge to elucidate their functions. Herein, we use integrative genomic, epigenomic and transcriptomic profiling of perturbed human coronary artery smooth muscle cells and tissues to begin to identify causal regulatory variation and mechanisms responsible for CAD associations. Using these genome-wide maps, we prioritize 64 candidate variants and perform allele-specific binding and expression analyses at seven top candidate loci: 9p21.3, SMAD3, PDGFD, IL6R, BMP1, CCDC97/TGFB1 and LMOD1. We validate our findings in expression quantitative trait loci cohorts, which together reveal new links between CAD associations and regulatory function in the appropriate disease context.
Total synthesis of atropurpuran
Authors: Gong Jing , Chen Huan , Liu Xiao-Yu , Wang Zhi-Xiu , Nie Wei , Qin Yong 
Published: July 2016

AbstractDue to their architectural intricacy and biological significance, the synthesis of polycyclic diterpenes and their biogenetically related alkaloids have been the subject of considerable interest over the last few decades, with progress including the impressive synthesis of several elusive targets. Despite tremendous efforts, conquering the unique structural types of this large natural product family remains a long-term challenge. The arcutane diterpenes and related alkaloids, bearing a congested tetracyclo[5.3.3.04,9.04,12]tridecane unit, are included in these unsolved enigmas. Here we report a concise approach to the construction of the core structure of these molecules and the first total synthesis of (±)-atropurpuran. Pivotal features of the synthesis include an oxidative dearomatization/intramolecular Diels-Alder cycloaddition cascade, sequential aldol and ketyl-olefin cyclizations to assemble the highly caged framework, and a chemoselective and stereoselective reduction to install the requisite allylic hydroxyl group in the target molecule.
Fractalkine restores the decreased expression of StAR and progesterone in granulosa cells from patients with polycystic ovary syndrome
Authors: Huang Shuo , Pang Yanli , Yan Jie , Lin Shengli , Zhao Yue , Lei Li , Yan Liying , Li Rong , Ma Caihong , Qiao Jie 
Published: July 2016

AbstractLow progesterone levels are associated with luteal phase deficiency in women with polycystic ovary syndrome (PCOS). The mechanisms regulating progesterone biosynthesis in the granulosa cells from women with PCOS is largely unknown. Fractalkine is expressed in human ovaries, and is reported to regulate progesterone production in granulosa cells of healthy women. In the current study, we aimed to examine the role of fractalkine in women with PCOS. Reduced fractalkine levels were found in follicular fluid and granulosa cells, accompanied by decreased progesterone production and reduced steroidogenic acute regulatory protein (StAR) expression in the granulosa cells of patients with PCOS. Administration of fractalkine reversed the inhibition of progesterone and StAR expression. The mechanism mediating these effects may be associated with the inhibition of ERK activity in the granulosa cells from women with PCOS. Our findings revealed that fractalkine regulated steroidogenesis in follicular granulosa cells of women with PCOS.
In Vivo Investigation of the Effectiveness of a Hyper-viscoelastic Model in Simulating Brain Retraction
Authors: Li Ping , Wang Weiwei , Zhang Chenxi , An Yong , Song Zhijian 
Published: July 2016

AbstractIntraoperative brain retraction leads to a misalignment between the intraoperative positions of the brain structures and their previous positions, as determined from preoperative images. In vitro swine brain sample uniaxial tests showed that the mechanical response of brain tissue to compression and extension could be described by the hyper-viscoelasticity theory. The brain retraction caused by the mechanical process is a combination of brain tissue compression and extension. In this paper, we first constructed a hyper-viscoelastic framework based on the extended finite element method (XFEM) to simulate intraoperative brain retraction. To explore its effectiveness, we then applied this framework to an in vivo brain retraction simulation. The simulation strictly followed the clinical scenario, in which seven swine were subjected to brain retraction. Our experimental results showed that the hyper-viscoelastic XFEM framework is capable of simulating intraoperative brain retraction and improving the navigation accuracy of an image-guided neurosurgery system (IGNS).
Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage
Authors: Fehrenbach Sabrina , Novak Daniel , Bernhardt Mathias , Larribere Lionel , Boukamp Petra , Umansky Viktor , Utikal Jochen 
Published: July 2016

AbstractLineage-specific transcription factors determine the cell fate during development. Direct conversion of several cell types into other lineages has been achieved by the overexpression of specific transcription factors. Even cancer cells have been demonstrated to be amenable to transdifferentiation. Here, we identified a distinct set of transcription factors, which are sufficient to transform cells of the keratinocytic lineage to melanocyte-like cells. Melanocyte marker expression was induced and melanosome formation was observed in non-tumorigenic keratinocytes (HaCaT) and tumorigenic squamous cell carcinoma (MET-4) cells. Moreover, reduced proliferation, cell metabolism, invasion and migration were measuredin vitroin transdifferentiated MT-MET-4 cells. A loss of tumorigenic potential of squamous cell carcinoma cells could be due to the upregulation of the melanocyte differentiation associated gene IL-24. Our data show that cells from the keratinocytic lineage can be transdifferented into the melanocytic lineage and provide a proof of principle for a potential new therapeutic strategy.