bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bee bread - perspective source of bioactive compounds for future
Authors: Ivanišová Eva , Kačániová Miroslava , Frančáková Helena , Petrová Jana , Hutková Jana , Brovarskyi Valeryii , Velychko Serhii , Adamchuk Leonora , Schubertová Zuzana , Musilová Janette 
Published: December 2015

Bee bread is product with long history used mainly in folk medicine. Nowadays, bee bread is growing in commercial interest due to its high nutritional properties. The objective of this study was to determine biological activity of ethanolic extract of bee bread obtained from selected region of Ukraine - Poltava oblast, Kirovohrad oblast, Vinnica oblast, Kyiv oblast, Dnepropetrovsk oblast. The antioxidant activity was measured with the radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as phosphomolybdenum assay. Total polyphenol content was determined with Folin-Ciocalteau reagent and total flavonoid content by aluminium-chloride method. Secondary was also evaluated antimicrobial activity in bee bread samples with disc diffusion method and minimum inhibitory concentrations. Antioxidant activity expressed as mg TEAC per g of dry weight (Trolox equivalent antioxidant capacity) was the highest in bee bread from Poltava oblast in DPPH and also phosphomolybdenum method. Samples of bee bread contained high levels of total polyphenols (12.36 - 18.24 mg GAE - gallic acid equivalent per g of dry weight) and flavonoids (13.56 - 18.24 μg QE - quercetin equivalent per g of dry weight) with the best values of bee bread from Poltava oblast. An elevated level of antioxidant potential in the bee bread determines its biological properties, which conditioned of the biological active substances. The best antibacterial activity of bee bred with disc diffusion method was found against Bacillus thuringiensis CCM 19. The antibacterial activity inhibited by the bee bread extract in the present study indicate that best minimal inhibition concentration was against bacteria Escherichia coli CCM 3988 and Salmonella enterica subs. enterica CCM 3807.
Selenium content increasing in the seeds of garden pea after foliar biofortification
Authors: Hegedüsová Alžbeta , Mezeyová Ivana , Hegedűs Ondrej , Musilová Janette , Paulen Oleg 
Published: December 2015

Selenium plays an important role as an antioxidant in the prevention of cardiovascular disease. Content of selenium in the crops is constantly in the spotlight of professional public. Vegetables, as an important source of chemo protective substances, have irreplaceable position within the food of plant character. The aim of research work was to solve the Se content increasing in the seeds of garden pea (varieties Premium and Ambassador) through the foliar biofortification of the plants (50 g Se / ha and 100 g Se / ha) and to monitor its effect on production of photosynthetic pigments. In the seeds of fresh garden pea, the chlorophyll a and chlorophyll b content was determined by spectrophotometer depending on a variety and the doses of selenium. In lyophilized seeds there was measured content of selenium by ET-AAS methods. The statistically significant increase of selenium was confirmed with its increasing concentrations in case of both varieties. In the var. Ambassador there was increasing from 0.083 ±0.009 mg.kg-1 DM to 4.935 ±0.598 mg.kg-1 DM (60-fold) and in a var. Premium the values increase from 0.067±0.007 mg.kg-1 DM to 3.248 ±0.289 mg.kg-1 DM (48-fold) after application of 100 g Se / ha. After application of 50 g Se / ha in both varieties of peas there was reported 25-fold increasing in the selenium content in comparison with control. The content of photosynthetic pigments was also increased, or possibly left at level of un-fortificated variant (chla - Ambassador - 50 g Se / ha; chlb - Premium - 100 g Se / ha) by foliar biofortification. Chlorophyll a content was high significantly increased according to used statistical methods in varieties Premium, from the content of 24.527 ±5.156 mg.kg-1 FM to 66.953 ±6.454 mg.kg-1 FM, likewise the content of chlorophyll b from the value of 19.708 ±5.977 mg.kg-1 FM to 37.488 ±6.146 mg.kg-1 FM (after 50 g / ha application). Foliar biofortification of different vegetable species can provide large-scale intake of minerals with antioxidant properties for human as well as an increase of certain biologically active substances as a result of their synergies
The effect of dietary alfalfa meal on the chicken meat quality
Authors: Tkáčová Jana , Haščí­k Peter , Angelovičová Mária , Pavelkova Adriana , Bobko Marek 
Published: December 2015

The purpose of this study was to investigate the effect of feed mixtures with proportion of alflafa meal 4% on body weight of broiler chickens, fat content their meat and oxidative stability meat fat under storage conditions. Final hybrid Cobb 500 chickens were used in the experiment. The broiler chickens were fed with feed mixtures starter from the 1st to the 18th day, grower from the 19th to the 31st day and finisher from the 32nd to the 38th ad libitum. An alfalfa meal of 4% was added in the feed mixtures of experimental group. In our experiment, we used alfalfa meal, which was made from Medicago sativa L. harvested in the bud's phase. The carcasses of broilers were stored at -18° C for a period of nine months. The average weight of chickens at the end of our experiment, the control group was 1685.6 g. In experimental group with addition of 4% alfalfa meal in feed mixtures, the chickens weighed 1709.6 g. The difference in body weight between the groups was not statistically significant (p >0.05). The average fat content in chicken meat was lower in the group with a share of 4% alfalfa meal 2.33 g.100g-1 compared to the fat content in meat chickens control group 2.59 g.100g-1. Differences in fat content in meat between the groups were not statistically significant (p >0.05). In our experiment, the average peroxide value of fat in meat under storage conditions 9 months at -18°C was lower in the group with a share of 4% alfalfa meal 2.42 µmol O2.g-1 compared with an average value of peroxide number 5.79 µmol O2.g-1 in the control group. Medicago sativa L. is an interesting object for research. It is characterized by high content of protein and biologically active substances that are effective for the promotion of health, and also an improvement the nutritional value and technological properties of the poultry food, when is used in feed mixtures.
Vernacular Traditions: Exploring Anglo-Saxon Mentalities
Authors: Matto Michael 
Published: December 2015

Abstract
Two recent books on Anglo-Saxon ideas about psychology have variants of the word “tradition” in their titles and develop theoretical paradigms around that term: Leslie Lockett’s Anglo-Saxon Psychologies in the Vernacular and Latin Traditions (2011) and Britt Mize’s Traditional Subjectivities: The Old English Poetics of Mentality (2013), both from the University of Toronto Press.1 While not primarily about the nature of traditions,2 both books rely on that term to frame their discussions of the psychology of the self and of how subjectivity manifests within different fields of Anglo-Saxon culture. In the earlier of the two books, Lockett offers a comprehensive account of Anglo-Saxon philosophical and theological concerns with the relationship among body, mind, and soul. In doing so she elaborates a distinction between two psychological models (and their variations) that were influential and productive in the Anglo-Saxon period. Mize’s project is narrower and deeper: he argues that the definition of the Anglo-Saxon poetic tradition itself should include a thematic and formulaic preoccupation with mentality. To read these books side-by-side is to see two authors’ projects weaving in and out of one another’s areas of interest and, in doing so, illustrating how fruitful psychology and mentality in Anglo-Saxon England can be as research topics. At the same time, they remind us how difficult it is to perceive pre-Christian “traditional” attitudes—whether about poetic conventions or psychological ideas—in the relatively late manuscript evidence we have.
VAGrENT: Variation Annotation Generator
Authors: Menzies Andy , Teague Jon W. , Butler Adam P. , Davies Helen , Tarpey Patrick , Nik‐Zainal Serena , Campbell Peter J. 
Published: December 2015

AbstractVAGrENT is a tool that provides biological context and effect prediction for genomic sequence variants. It annotates single base substitutions and small insertions and deletions by comparing them to reference information within or close to genes or other transcribed elements. This information provides the critical insight required to inform the biological or clinical significance of variant data generated from sequencing studies. The software has been optimized to run efficiently against the large numbers and diverse classes of variants that are typically generated from next generation sequencing technologies. This unit describes how to configure and use VAGrENT and also contains support protocols for extending and adapting its default behavior. © 2015 by John Wiley & Sons, Inc.
Rapid and highly variable warming of lake surface waters around the globe
Authors: O'Reilly Catherine M. , Sharma Sapna , Gray Derek K. , Hampton Stephanie E. , Read Jordan S. , Rowley Rex J. , Schneider Philipp , Lenters John D. , McIntyre Peter B. , Kraemer Benjamin M. , Weyhenmeyer Gesa A. , Straile Dietmar , Dong Bo , Adrian Rita , Allan Mathew G. , Anneville Orlane , Arvola Lauri , Austin Jay , Bailey John L. , Baron Jill S. , Brookes Justin D. , de Eyto Elvira , Dokulil Martin T. , Hamilton David P. , Havens Karl , Hetherington Amy L. , Higgins Scott N. , Hook Simon , Izmest'eva Lyubov R. , Joehnk Klaus D. , Kangur Kulli , Kasprzak Peter , Kumagai Michio , Kuusisto Esko , Leshkevich George , Livingstone David M. , MacIntyre Sally , May Linda , Melack John M. , Mueller‐Navarra Doerthe C. , Naumenko Mikhail , Noges Peeter , Noges Tiina , North Ryan P. , Plisnier Pierre‐Denis , Rigosi Anna , Rimmer Alon , Rogora Michela , Rudstam Lars G. , Rusak James A. , Salmaso Nico , Samal Nihar R. , Schindler Daniel E. , Schladow S. Geoffrey , Schmid Martin , Schmidt Silke R. , Silow Eugene , Soylu M. Evren , Teubner Katrin , Verburg Piet , Voutilainen Ari , Watkinson Andrew , Williamson Craig E. , Zhang Guoqing 
Published: December 2015

AbstractIn this first worldwide synthesis of in situ and satellite‐derived lake data, we find that lake summer surface water temperatures rose rapidly (global mean = 0.34°C decade−1) between 1985 and 2009. Our analyses show that surface water warming rates are dependent on combinations of climate and local characteristics, rather than just lake location, leading to the counterintuitive result that regional consistency in lake warming is the exception, rather than the rule. The most rapidly warming lakes are widely geographically distributed, and their warming is associated with interactions among different climatic factors—from seasonally ice‐covered lakes in areas where temperature and solar radiation are increasing while cloud cover is diminishing (0.72°C decade−1) to ice‐free lakes experiencing increases in air temperature and solar radiation (0.53°C decade−1). The pervasive and rapid warming observed here signals the urgent need to incorporate climate impacts into vulnerability assessments and adaptation efforts for lakes.
Lunar water migration in the interval between large impacts: Heterogeneous delivery to Permanently Shadowed Regions, fractionation, and diffusive barriers
Authors: Moores John E. 
Published: December 2015

AbstractThe migration of water molecules across the lunar surface from sources sufficiently small, so as to not generate a transient collisional atmosphere, was examined using a Monte Carlo simulation. Previous work using similar models is extended by examining a realistic distribution of large south polar Permanently Shadowed Regions (PSRs) to examine the heterogeneities observed in their water content—frost in Haworth and the nearby lowlands, but not in neighboring Shoemaker, and anomalously high hydrogen abundances from neutron data—and to determine how the pattern of water delivery can be distinguished from that predicted for transient collisional atmospheres. While the Haworth lowlands were seen to accumulate 3.9 times as much water as Shoemaker, the additional water delivered to Haworth crater was only 47% greater than Shoemaker, possibly due to shielding by Faustini and the lowlands. This relatively small difference is likely inadequate to explain the difference in frosting. More broadly, latitudinal trends in delivery dominate over meridional trends. Despite the heterogeneity in water delivery, fractionation effects from ballistic migration were small for the PSRs with equal D/H ratios seen in all PSRs within confidence limits. Finally, a diffusive barrier was observed starting at approximately 15° from each pole that hampers the poleward progress of equatorial particles. This barrier is the result of a reduction in the migration diffusivity from 1.0 × 107 m2 s−1 near the equator to 5.5 × 105 m2 s−1 within 10° of the pole and finally to 6.8 × 103 m2 s−1 within 4° of the pole.
Copper‐Catalyzed Aerobic Formation of Unstable Sulfinyl Radicals for the Synthesis of Sulfinates and Thiosulfonates
Authors: Shyam Pranab K. , Kim Yu Kwon , Lee Chan , Jang Hye‐Young 
Published: December 2015

AbstractCopper‐catalyzed aerobic coupling of thiols and alcohols affords sulfinates and thiosulfonates. These products are assumed to form via sulfinyl radicals which are not commonly found in oxidative coupling reactions of thiols. A reaction mechanism involving sulfinyl radicals is proposed, and mass and electron paramagnetic resonance (EPR) experimental results are provided.magnified image
T2R38 genotype is correlated with sinonasal quality of life in homozygous ΔF508 cystic fibrosis patients
Authors: Adappa Nithin D. , Workman Alan D. , Hadjiliadis Denis , Dorgan Daniel J. , Frame Danielle , Brooks Steven , Doghramji Laurel , Palmer James N. , Mansfield Corrine , Reed Danielle R. , Cohen Noam A. 
Published: December 2015

BackgroundChronic rhinosinusitis (CRS) is very prevalent in the cystic fibrosis (CF) patient population, and leads to high morbidity and markedly decreased quality of life (QOL). Identification of genetic markers that contribute to CRS symptoms in these patients can allow for risk stratification and tailoring of medical and surgical treatments. T2R38 is a bitter taste receptor expressed in the sinonasal tract, and nonfunctional alleles of this receptor have been implicated in treatment‐refractory CRS in non‐CF patients. The purpose of this study is to investigate the significance of T2R38 genotype in the variability of sinonasal QOL and CRS disease severity in a sample of CF patients.MethodsΔF508 homozygous CF patients were recruited from the University of Pennsylvania Cystic Fibrosis Center and were genotyped for the TAS2R38 locus. To assess sinonasal symptom severity, a 22‐item Sino‐Nasal Outcome Test (SNOT‐22) was collected from each patient. Additional demographic and medical history data was obtained at the time of patient enrollment.ResultsA total of 49 ΔF508 homozygous CF patients aged 18 to 32 years were included in the final SNOT‐22 score analysis. Individuals with 2 functional T2R38 alleles (PAV/PAV) had significantly lower SNOT‐22 scores (n = 49, p < 0.05). On further breakdown of SNOT‐22 subcategories, rhinologic symptoms specifically were less severe in PAV/PAV patients than patients with other genotypes (n = 47, p < 0.05).ConclusionOur investigation indicates that T2R38 genotype correlates both with SNOT‐22 scores and rhinologic‐specific QOL in ΔF508 homozygous CF patients.
Zur Kenntnis von Lösungssystemen in tief siedenden Lösungsmitteln: 1. Mitteilung
Authors: Schenk Peter W. , Tulhoff Helmut 
Published: December 2015

AbstractEine neue Methode zur Löslichkeitsbestimmung in verflüssigten Gasen bei niedrigen Temperaturen wird beschrieben. Sie beruht darauf, daß aus einem Vorratsvolumen auf die zu lösende Substanz bei konstanter Temperatur so lange Lösungsmittel aufkondensiert wird, bis eine gesättigte Lösung entstanden ist. Aus der Druckabnahme im Vorratsgefäß wird die Lösungsmittelmenge bestimmt. Die Methode wird an den bereits untersuchten Systemen KCl/NH3, KBr/NH3, KJ/NH3 und KNO3/NH3 geprüft und die erhaltenen Werte in guter Übereinstimmung mit den für diese Systeme bekannten Werten gefunden.
Magnetic Susceptibility of Liquid Se – Te, Se – Tl, and Se – Ge Mixtures
Authors: Misonou M. , Endo H. 
Published: December 2015

AbstractThe measurements of magnetic susceptibility have been done for liquid Se – Te, Se – Tl, and Se – Ge mixtures up to 1000 °C. The number of the broken bonds per mole and the energy required to break a chain are deduced as functions of temperature and solute concentration. The modification of the chain structure of liquid Se by the addition of Te, Tl, or Ge is discussed.
Brain‐derived neurotropic factor polymorphisms, traumatic stress, mild traumatic brain injury, and combat exposure contribute to postdeployment traumatic stress
Authors: Dretsch Michael N. , Williams Kathy , Emmerich Tanja , Crynen Gogce , Ait‐Ghezala Ghania , Chaytow Helena , Mathura Venkat , Crawford Fiona C. , Iverson Grant L. 
Published: December 2015

AbstractBackgroundIn addition to experiencing traumatic events while deployed in a combat environment, there are other factors that contribute to the development of posttraumatic stress disorder (PTSD) in military service members. This study explored the contribution of genetics, childhood environment, prior trauma, psychological, cognitive, and deployment factors to the development of traumatic stress following deployment.MethodsBoth pre‐ and postdeployment data on 231 of 458 soldiers were analyzed. Postdeployment assessments occurred within 30 days from returning stateside and included a battery of psychological health, medical history, and demographic questionnaires; neurocognitive tests; and blood serum for the D2 dopamine receptor (DRD2), apolipoprotein E (APOE), and brain‐derived neurotropic factor (BDNF) genes.ResultsSoldiers who screened positive for traumatic stress at postdeployment had significantly higher scores in depression (d = 1.91), anxiety (d = 1.61), poor sleep quality (d = 0.92), postconcussion symptoms (d = 2.21), alcohol use (d = 0.63), traumatic life events (d = 0.42), and combat exposure (d = 0.91). BDNF Val66 Met genotype was significantly associated with risk for sustaining a mild traumatic brain injury (mTBI) and screening positive for traumatic stress. Predeployment traumatic stress, greater combat exposure and sustaining an mTBI while deployed, and the BDNF Met/Met genotype accounted for 22% of the variance of postdeployment PTSD scores (R2 = 0.22, P < 0.001). However, predeployment traumatic stress, alone, accounted for 17% of the postdeployment PTSD scores.ConclusionThese findings suggest predeployment traumatic stress, genetic, and environmental factors have unique contributions to the development of combat‐related traumatic stress in military service members.
Artificial Metalloenzymes with the Neocarzinostatin Scaffold: Toward a Biocatalyst for the Diels–Alder Reaction
Authors: Ghattas Wadih , Cotchico‐Alonso Lur , Maréchal Jean‐Didier , Urvoas Agathe , Rousseau Maëva , Mahy Jean‐Pierre , Ricoux Rémy 
Published: December 2015

AbstractA copper(II) cofactor coupled to a testosterone anchor, copper(II)‐(5‐(Piperazin‐1‐yl)‐1,10‐phenanthroline)testosterone‐17‐hemisuccinamide (10) was synthesized and associated with a neocarzinostatin variant, NCS‐3.24 (KD=3 μm), thus generating a new artificial metalloenzyme by following a “Trojan horse” strategy. Interestingly, the artificial enzyme was able to efficiently catalyze the Diels–Alder cyclization reaction of cyclopentadiene (1) with 2‐azachalcone (2). In comparison with what was observed with cofactor 10 alone, the artificial enzymes favored formation of the exo products (endo/exo ratios of 84:16 and 62:38, respectively, after 12 h). Molecular modeling studies assigned the synergy between the copper complex and the testosterone (KD=13 μm) moieties in the binding of 10 to good van der Waals complementarity. Moreover, by pushing the modeling exercise to its limits, we hypothesize on the molecular grounds that are responsible for the observed selectivity.
A Rapid and Versatile Assay for Ago2‐Mediated Cleavage by Using Branched Rolling Circle Amplification
Authors: Hesse Marlen , Arenz Christoph 
Published: December 2015

AbstractMicro RNA (miRNA) research has evolved into an essential part of investigating gene regulation in which deregulation of numerous miRNAs is associated with various cellular dysfunction and diseases. Here, we describe a rapid and homogenous assay for Ago2‐mediated target RNA cleavage, based on branched rolling circle amplification (BRCA). In particular, the ability to investigate small molecule binders for inhibition of miRNA function is within the potential of our assay. This method uses no artificial fluorescence labeling of RNA components, which can be an advantage in screening of potential inhibitors. To visualize cleavage of RNA substrate by Ago2, we developed a two‐step assay composed of Ago2‐mediated cleavage and BRCA‐based detection. The assay is cost‐effective and practicable and can be performed in 96‐well format by using a standard qPCR machine.
Activation and Transformation of Ethane by Au2VO3+ Clusters with Closed‐Shell Electronic Structures
Authors: Li Ya‐Ke , Li Zi‐Yu , Zhao Yan‐Xia , Liu Qing‐Yu , Meng Jing‐Heng , He Sheng‐Gui 
Published: December 2015

AbstractThe study of chemical reactions between gold‐containing heteronuclear oxide clusters and small molecules can provide molecular level mechanisms to understand the excellent activity of gold supported by metal oxides. While the promotion role of gold in alkane transformation was identified in the clusters with atomic oxygen radicals (O−.), the role of gold in the systems without O−. is not clear. By employing mass spectrometry and quantum chemistry calculations, the reactivity of Au2VO3+ clusters with closed‐shell electronic structures toward ethane was explored. Both the dehydrogenation and ethene elimination channels were identified. It is gold rather than oxygen species initiating the C−H activation. The Au−Au dimer formed during the reactions plays important roles in ethane transformation. The reactivity comparison between Au2VO3+ and bare Au2+ demonstrates that Au2VO3+ not only retains the property of bare Au2+ that transforming ethane to dihydrogen, but also exhibits new functions in converting ethane to ethene, which reveals the importance of the composite system. This study provides a further understanding of the reactivity of metal oxide supported gold in alkane activation and transformation.