bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery and Evaluation of Biosynthetic Pathways for the Production of Five Methyl Ethyl Ketone Precursors
Authors: Tokic Milenko , Hadadi Noushin , Ataman Meric , Neves Dário , Ebert Birgitta E. , Blank Lars M. , Miskovic Ljubisa , Hatzimanikatis Vassily 
Published: October 2017

AbstractThe limited supply of fossil fuels and the establishment of new environmental policies shifted research in industry and academia towards sustainable production of the 2ndgeneration of biofuels, with Methyl Ethyl Ketone (MEK) being one promising fuel candidate. MEK is a commercially valuable petrochemical with an extensive application as a solvent. However, as of today, a sustainable and economically viable production of MEK has not yet been achieved despite several attempts of introducing biosynthetic pathways in industrial microorganisms. We used BNICE.ch as a retrobiosynthesis tool to discover all novel pathways around MEK. Out of 1’325 identified compounds connecting to MEK with one reaction step, we selected 3-oxopentanoate, but-3-en-2-one, but-1-en-2-olate, butylamine, and 2-hydroxy-2-methyl-butanenitrile for further study. We reconstructed 3’679’610 novel biosynthetic pathways towards these 5 compounds. We then embedded these pathways into the genome-scale model ofE. coli, and a set of 18’622 were found to be most biologically feasible ones based on thermodynamics and their yields. For each novel reaction in the viable pathways, we proposed the most similar KEGG reactions, with their gene and protein sequences, as candidates for either a direct experimental implementation or as a basis for enzyme engineering. Through pathway similarity analysis we classified the pathways and identified the enzymes and precursors that were indispensable for the production of the target molecules. These retrobiosynthesis studies demonstrate the potential of BNICE.ch for discovery, systematic evaluation, and analysis of novel pathways in synthetic biology and metabolic engineering studies.Graphical abstract
Control of septin filament flexibility and bundling
Authors: Khan Anum , Newby Jay , Gladfelter Amy S. 
Published: October 2017

AbstractSeptins self-assemble into heteromeric rods and filaments to act as scaffolds and modulate membrane properties. How cells tune the biophysical properties of septin filaments to control filament flexibility and length, and in turn the size, shape, and position of higher-order septin structures is not well understood. We examined how rod composition and nucleotide availability influence physical properties of septins such as annealing, fragmentation, bundling and bending. We found that septin complexes have symmetric termini, even when both Shs1 and Cdc11 are coexpressed. The relative proportion of Cdc11/Shs1 septin complexes controls the biophysical properties of filaments and influences the rate of annealing, fragmentation, and filament flexibility. Additionally, the presence and exchange of guanine nucleotide also alters filament length and bundling. An Shs1 mutant that is predicted to alter nucleotide hydrolysis has altered filament length and dynamics in cells and impacts cell morphogenesis. These data show that modulating filament properties through rod composition and nucleotide binding can control formation of septin assemblies that have distinct physical properties and functions.
Nanopore Sequencing Reveals High-Resolution Structural Variation in the Cancer Genome
Authors: Gong Liang , Wong Chee-Hong , Cheng Wei-Chung , Tjong Harianto , Menghi Francesca , Ngan Chew Yee , Liu Edison T. , Wei Chia-Lin 
Published: October 2017

AbstractAcquired genomic structural variants (SVs) are major hallmarks of the cancer genome. Their complexity has been challenging to reconstruct from short-read sequencing data. Here, we exploit the long-read sequencing capability of the nanopore platform using our customized pipeline,Picky, to reveal SVs of diverse architecture in a breast cancer model. From modest sequencing coverage, we identified the full spectrum of SVs with superior specificity and sensitivity relative to short-read analyses and uncovered repetitive DNA as the major source of variation. Examination of the genome-wide breakpoints at nucleotide-resolution uncovered micro-insertions as the common structural features associated with SVs. Breakpoint density across the genome is associated with propensity for inter-chromosomal connectivity and transcriptional regulation. Furthermore, an over-representation of reciprocal translocations from chromosomal double-crossovers was observed through phased SVs. The comprehensive characterization of SVs using the robust long-read sequencing approach in cancer cohorts will facilitate strategies to monitor genome stability during tumor evolution and improve therapeutic intervention.
Hobson’s choice: the effects of research evaluation on academics’ writing practices in England
Authors: Mcculloch Sharon 
Published: October 2017

Purpose
The purpose of this paper is to examine the influence of research evaluation policies and their interpretation on academics’ writing practices in three different higher education institutions and across three different disciplines. Specifically, the paper discusses how England’s national research excellence framework (REF) and institutional responses to it shape the decisions academics make about their writing.

Design/methodology/approach
In total, 49 academics at three English universities were interviewed. The academics were from one Science, Technology, Engineering and Mathematics discipline (mathematics), one humanities discipline (history) and one applied discipline (marketing). Repeated semi-structured interviews focussed on different aspects of academics’ writing practices. Heads of departments and administrative staff were also interviewed. Data were coded using the qualitative data analysis software, ATLAS.ti.

Findings
Academics’ ability to succeed in their career was closely tied to their ability to meet quantitative and qualitative targets driven by research evaluation systems, but these were predicated on an unrealistic understanding of knowledge creation. Research evaluation systems limited the epistemic choices available to academics, partly because they pushed academics’ writing towards genres and publication venues that conflicted with disciplinary traditions and partly because they were evenly distributed across institutions and age groups.

Originality/value
This work fills a gap in the literature by offering empirical and qualitative findings on the effects of research evaluation systems in context. It is also one of the only papers to focus on the ways in which individuals’ academic writing practices in particular are shaped by such systems.
Establishing the financial returns arising from an evaluation of a retail training programme
Authors: Olexová Cecília 
Published: October 2017

Purpose
The purpose of this paper is to propose an evaluation of training for shop assistants, specifically in retail chains.

Design/methodology/approach
The proposed evaluation is based on Kirkpatrick’s model and Phillips’ modification. The representative case study includes the application of the training evaluation at the fourth and fifth levels of evaluation in a particular chain of retail stores. The data were collected from the retail chain’s financial reports, internal documents related to training of employees and interviews with managers in the retail chain.

Findings
The results contribute to the training evaluation literature by presenting a training evaluation for shop assistant using evidence from a retail chain. The training focussed on selling skills and product knowledge; the details of evaluating the training are presented, encompassing mainly the benefits and costs of the training and return on investment calculation.

Research limitations/implications
The training evaluation was tested only in selected types of training courses for shop assistants. To generalise the results, its adoption in different types of training and retail companies is required in future research.

Practical implications
The application of this training evaluation should help to achieve training objectives and enhance the quality of training in the retail chain. The results of the paper can be used as practical inspiration for managers of retail stores.

Originality/value
This paper proposes and applies a specific evaluation of training courses in retail.
Layers of difference: a cancer center’s approach to minimizing cancer health disparity
Authors: Chachula Desiree , Grant Cathy , Antolino Prado , Davis Jenna , Hanson Desiree , Harris Lesley , Rivera-Colon Venessa , Green B. Lee 
Published: October 2017

Purpose
The purpose of this paper is to provide a case study of a multifaceted institutional approach to minimizing cancer health disparities, presenting a novel organizational framework entitled, “A.C.C.E.S.S.” to guide those efforts.

Design/methodology/approach
This paper presents a case study of an organization that operates under the theory that cancer health disparities are a result of the cumulative incongruence of differences that exist between people in various contexts and interactions over time. Consequently, the A.C.C.E.S.S. framework is used to demonstrate the range of opportunities within an organization to intervene and mitigate gaps that result in inequality.

Findings
Addressing A.C.C.E.S.S. in various interactions and contexts over a sustained period of time results in a continuous improvement cycle that attenuates cancer health disparity.

Originality/value
The antecedents and impacts of cancer health disparities are well documented. However, there is a dearth of directionality for institutions and organizations in achieving equality in cancer treatment and care. This paper provides a framework to consider in organizing such endeavors.
Translation, adaptation, and cross-language validation of student: thinking about my homework scale (STP)
Authors: Aqeel Muhammad , Jami Humaira , Ahmed Ammar 
Published: October 2017

Purpose
The purpose of this paper is to establish the reliability and validity of an expended scale with translation, adaptation and cross-language validation of the student: thinking about my homework (STP) (Bareno, 1997; Hoover-Dempsey et al., 1999; Hoover-Dempsey and Sandler, 2005).

Design/methodology/approach
Response items rating are made from four-point Likert-type scales ranging from the 1 (never) to 4 (always). This study includes two versions of the scale: mother’s school involvement version (STPM) and father’s school Involvement version (STPF). Both versions have been translated from English language into Urdu language with a sample of 200 students. Standard back translation method was used for translation and adaption of the scale (Brislin, 1976; Hambleton, 1994). The ages of the students ranged from 12 to 18 years.

Findings
The overall scale has good internal consistency reliability. Exploratory factor analysis was performed to explore covert and novel configuration of these versions (father’s school involvement and mother’s school involvement). Results revealed that mother’s school involvement consisted of 21 items, and father’s school involvement consisted of 22 items.

Originality/value
The investigated scale provides assessment of father’s and mother’s school involvement, respectively, in order to achieve better understanding of family’s role in academics.
A critical review of current police training and policy for autism spectrum disorder
Authors: Hepworth Diana 
Published: October 2017

Purpose
The purpose of this paper is to critically review the current police training and criminal justice policy regarding the treatment of suspects with autism spectrum disorder (ASD) during the initial stages of the criminal justice system (CJS), and provide potential policy reform and areas for further research.

Design/methodology/approach
By reviewing extant literature, research and policy documents, this paper provides a critical review of the current policy and training for dealing with suspects with ASD in the current CJS in England and Wales for suspects with ASD.

Findings
This paper proposes that current policy and police staff training is insufficient during all initial stages of the criminal justice process. Although there are emerging policies and schemes which are promising, they require further research and national participation. Policy reform and improved training is required to ensure minimal opportunities for miscarriages of justice to those individuals with ASD.

Originality/value
This paper provides a chronological journey through the initial stages of the CJS in England and Wales for a suspect with ASD, and the challenges that they may face. Suggestions are made based on criminological and psychological research to remedy the potential opportunities for miscarriages of justice.
Revealing the training on intellectual and developmental disabilities among forensic mental health professionals: a survey report
Authors: Sarrett Jennifer C. 
Published: October 2017

Purpose
The purpose of this paper is to assess the training forensic mental health professionals in the USA receive on intellectual and developmental disabilities (I/DD). Given the difficulties obtaining accurate prevalence rates of these disabilities in criminal justice settings, it is important to understand how these disabilities are being evaluated and the level of understanding about these disabilities evaluators hold.

Design/methodology/approach
An online survey was distributed to forensic mental health professionals in the USA that included questions on training opportunities in graduate education, post-graduate forensic training, and professional training opportunities. Participants were also asked about their current work, how they assess I/DD, and their estimates on the percentage of cases they see with I/DD.

Findings
Respondents reported some training that focused heavily on assessment methods. Most respondents estimated between 5 and 25 percent of their cases involving I/DD and reported using a wide range of assessment methods. Finally, many respondents reporting more training needed in this area.

Practical implications
More training is needed for forensic mental health professionals on identifying I/DD. Additionally, professional guidelines on what tools and methods to rely on to identify these disabilities is paramount to ensure homogeneity of methods and, thus, better estimates of overall prevalence in criminal justice settings.

Originality/value
This is the first assessment focused on how forensic mental health professionals are trained to identify I/DD and can be used to improve identification of I/DD in forensic settings.
ASAP-ID: substance abuse programme for a forensic ID population
Authors: Sakdalan Joseph Allan , Kittner Daniel McGarry , Judd Devika 
Published: October 2017

Purpose
There are a lack of recourses for substance abuse (SA) treatment for forensic clients with intellectual disabilities (ID). Many complexities arise when treating this population, calling for the creation of comprehensive resources which not only address the SA, but also account for the risk and offending issues. The purpose of this paper is to detail a pilot programme which aims to provide treatment for forensic ID clients with substance abuse issues as well as a significant risk of reoffending.

Design/methodology/approach
Six participants completed a 27-week SA treatment programme (the Alcohol and Substance Abuse Programme-Intellectual Disability) which incorporated the use of dialectical behavioural therapy (DBT) and the Good Lives Model concepts. Pre- and post-measures aimed to assess readiness for change and confidence in ability to stay clean and sober.

Findings
Preliminary findings showed a marked improvement in confidence of the participants’ ability to stay clean and sober in risk-related situations as well as an increase in overall readiness for change.

Originality/value
This research paper addresses a gap in the current forensic ID research and clinical treatment options pertaining to SA, by focussing on supporting forensic ID clients in their recovery journey from SA. Being at the forefront of SA treatment for forensic ID, further research in this domain should attempt to consolidate the findings of this programme.
Humanistic perspectives in virtual reference
Authors: Prieto Adolfo G. 
Published: October 2017

Purpose
Virtual reference is a part of the services offered at academic and other types of libraries. This paper discusses humanistic perspectives that merit consideration in the provision of virtual reference (VR), including mindfulness, authenticity, emotional intelligence (EI) and counseling. This study aims to make these perspectives become a part of the language and practice of virtual reference through this discussion.

Design/methodology/approach
The paper establishes a framework for the VR perspectives it discusses by examining relevant literature for background information and practical applications.

Findings
VR has the potential to benefit from the interrelated humanistic perspectives of mindfulness, authenticity, EI and counseling. It is through the counseling perspective that mindfulness, authenticity and EI are embodied to offer a more transformational and less transactional experience for both librarians and users.

Research limitations/implications
While the paper provides examples of language used by the author to illustrate the counseling perspective, a future study, whereby virtual reference transcripts are formally analyzed, may be beneficial to determine other expressions of counseling and its related perspectives of mindfulness, authenticity and EI.

Practical implications
This paper provides a resource especially helpful to individuals new to VR or who seek opportunities for growth in providing VR.

Originality/value
In addition to taking a multifaceted view of VR through the humanistic perspectives presented, this paper also offers a multidisciplinary approach in its discussion, drawing from areas such as business and medicine.
Embedding digital and research-literacy support program into postgraduate studies curriculum
Authors: Besseah Bernard , Achiro Daisy , Mhando Joseph , Salau Sadiat Adetoro 
Published: October 2017

Purpose
This viewpoint paper aims to propose a digital and research literacy support program for postgraduate schools in sub-Saharan Africa institutions.

Design/methodology/approach
The paper reviewed literature on postgraduate students’ skills and postgraduate information literacy programs and proposed one for postgraduate schools in sub-Saharan Africa institutions.

Findings
Information literacy programs are not implemented at the postgraduate level in sub-Saharan Africa possibly because of lack of contents for these programs.

Research limitations/implications
The course is only a proposed course that can be embedded into the postgraduate curriculum in sub-Saharan African universities. The effectiveness of the course has not been evaluated in this study.

Originality/value
The proposed information literacy program focused on digital and research literacy, which is still relatively new in sub-Saharan Africa.
Accessibility of women to health information in Tanzania
Authors: Benard Ronald , Chipungahelo Monica Samwel 
Published: October 2017

Purpose
The aim of this study is to examine accessibility of health information to women in Tanzania with reference to the Morogoro region. The specific objectives of the study were: first, to identify the health information needs of women; second, to determine the accessibility of the needed health information to women in the study area; and third, to determine the preferred sources of information used by women in accessing health information in the study area.

Design/methodology/approach
A descriptive survey method – cross-sectional design – was used. Semi-structured questionnaires with both open- and close-ended questions were used to collect data from four wards of Morogoro Municipal Council, Tanzania. Key informant interviews were conducted with 12 women from four wards, 3 women were selected from each ward.

Findings
The findings also indicated that there was a significant relationship between wards and accessibility to certain types of information which were concerning hypertension, family planning, malaria and typhoid. Although information on diabetes and hypertension had lower percentages of accessibility in all four wards, the study findings revealed that medical doctors, pharmacy shops and family were the main sources of information used by women to access health information. Radio and television were rated as preferred sources of information required by women, whereas internet, local herb hawkers and mobile phones were rated as non-preferable. It is therefore recommended that the government through health-care providers and medical librarians should be proactive in creating awareness and disseminate health information on non-communicable diseases such as hypertension and diabetes to women.

Practical implications
This paper provides practical recommendations on how to improve accessibility of health information in the communities.

Social implications
The paper has an implication of improving accessibility of health information to women in the communities.

Originality/value
The paper provides appropriate knowledge that is needed in improving access to health information in Tanzanian communities and in other developing countries communities.
Social influence and cognitive instrumental factors as facilitators of perceived usefulness of electronic resources among library personnel in private universities in South-west, Nigeria
Authors: Izuagbe Roland , Popoola Sunday Olanrewaju 
Published: October 2017

Purpose
The study examined the applicability of TAM2 theoretical constructs of social influence and cognitive instrumental factors vis-à-vis the facilitation of perceived usefulness of electronic resources among library personnel in private universities in South-west Nigeria.

Design/Methodology/Approach
Descriptive research design of correlational type alongside the single-stage random sampling technique were used to select 212 respondents from a population size of 259 library personnel across the 21 private universities in South-west Nigeria. The instrument used to elicit data was the questionnaire. Of the 212 copies of the questionnaire administered, 181 (85.4 per cent) were found valid for analysis.

Findings
Social influence and cognitive instrumental factors significantly had relationship with and facilitated perceived usefulness of electronic resources. Similarly, the study revealed that cognitive instrumental factors are stronger facilitators of perceived usefulness of electronic resources than social influence factors among library personnel in private universities in South-west, Nigeria.

Practical implications
Implementation of electronic resources without ascertaining library personnel usefulness perception leads to resource underutilisation and wastage. Management of private university libraries should, therefore, take into cognisance the usefulness perception of library personnel before the implementation of electronic resources for optimum utilisation.

Originality/value
There is no known research endeavour within the Nigerian context that examined perceived usefulness of electronic resources among library personnel in the private university domain, using the additional theoretical constructs of TAM2 as our research model suggests. This study is, therefore, a pioneering effort towards ascertaining library personnel’s perceived usefulness of electronic resources prior acceptance and use in private university libraries.