bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrated hyperspectral imaging and genome-wide association analysis platform provides spectral and genetic insights into the natural variation in rice
Authors: Feng Hui , Guo Zilong , Yang Wanneng , Huang Chenglong , Chen Guoxing , Fang Wei , Xiong Xiong , Zhang Hongyu , Wang Gongwei , Xiong Lizhong , Liu Qian 
Published: June 2017

AbstractWith progress of genetic sequencing technology, plant genomics has experienced rapid development and subsequently triggered the progress of plant phenomics. In this study, a high-throughput hyperspectral imaging system (HHIS) was developed to obtain 1,540 hyperspectral indices at whole-plant level during tillering, heading, and ripening stages. These indices were used to quantify traditional agronomic traits and to explore genetic variation. We performed genome-wide association study (GWAS) of these indices and traditional agronomic traits in a global rice collection of 529 accessions. With the genome-level suggestive P-value threshold, 989 loci were identified. Of the 1,540 indices, we detected 502 significant indices (designated as hyper-traits) that exhibited phenotypic and genetic relationship with traditional agronomic traits and had high heritability. Many hyper-trait-associated loci could not be detected using traditional agronomic traits. For example, we identified a candidate gene controlling chlorophyll content (Chl). This gene, which was not identified based on Chl, was significantly associated with a chlorophyll-related hyper-trait in GWAS and was demonstrated to control Chl. Moreover, our study demonstrates that red edge (680–760 nm) is vital for rice research for phenotypic and genetic insights. Thus, combination of HHIS and GWAS provides a novel platform for dissection of complex traits and for crop breeding.
Measurement of Stokes-operator squeezing for continuous-variable orbital angular momentum
Authors: Guo Jun , Cai Chunxiao , Ma Long , Liu Kui , Sun Hengxin , Gao Jiangrui 
Published: June 2017

AbstractWe demonstrate experimentally a measurement scheme for the Stokes operators for the continuous-variable squeezed states of orbital angular momentum (OAM). An OAM squeezed state is generated by coupling a dim Hermite-Gauss HG01-mode quadrature-squeezed light beam with a bright HG10-mode coherent light beam on a 98/2 beam splitter. Using an asymmetric Mach–Zehnder interferometer with an extra Dove prism in one arm, we measured the three orbital Stokes operators of the OAM squeezed states with a self-homodyne detection and finally characterized their positions and noise on the orbital Poincaré sphere.
EEG correlates of sensorimotor processing: independent components involved in sensory and motor processing
Authors: Melnik Andrew , Hairston W. David , Ferris Daniel P. , König Peter 
Published: June 2017

AbstractSensorimotor processing is a critical function of the human brain with multiple cortical areas specialised for sensory recognition or motor execution. Although there has been considerable research into sensorimotor control in humans, the steps between sensory recognition and motor execution are not fully understood. To provide insight into brain areas responsible for sensorimotor computation, we used complex categorization-response tasks (variations of a Stroop task requiring recognition, decision-making, and motor responses) to test the hypothesis that some functional modules are participating in both sensory as well as motor processing. We operationalize functional modules as independent components (ICs) yielded by an independent component analysis (ICA) of EEG data and measured event-related responses by means of inter-trial coherence (ITC). Our results consistently found ICs with event-related ITC responses related to both sensory stimulation and motor response onsets (on average 5.8 ICs per session). These findings reveal EEG correlates of tightly coupled sensorimotor processing in the human brain, and support frameworks like embodied cognition, common coding, and sensorimotor contingency that do not sequentially separate sensory and motor brain processes.
Evolution of Gene Expression in the Uterine Cervix related to Steroid Signaling: Conserved features in the regulation of cervical ripening
Authors: Wagner Günter P. , Nnamani Mauris C. , Chavan Arun Rajendra , Maziarz Jamie , Protopapas Stella , Condon Jennifer , Romero Roberto 
Published: June 2017

AbstractThe uterine cervix is the boundary structure between the uterus and the vagina and is key for the maintenance of pregnancy and timing of parturition. Here we report on a comparative transcriptomic study of the cervix of four placental mammals, mouse, guinea pig, rabbit and armadillo, and one marsupial, opossum. Our aim is to investigate the evolution of cervical gene expression as related to putative mechanisms for functional progesterone withdrawal. Our findings are: 1) The patterns of gene expression in eutherian (placental) mammals are consistent with the notion that an increase in the E/P4 signaling ratio is critical for cervical ripening. How the increased E/P4 ratio is achieved, however, is variable between species. 2) None of the genes related to steroid signaling, that are modulated in eutherian species, change expression during opossum gestation. 3) A tendency for decreased expression of progesterone receptor co-activators (NCOA1, -2 and -3, and CREBBP) towards term is a shared derived feature of eutherians. This suggests that parturition is associated with broad scale histone de-acetylation. Western-blotting on mouse cervix confirmed large scale histone de-acetylation in labor. This finding may have important implications for the control of premature cervical ripening and prevention of preterm birth in humans.
Heart Failure and MEF2 Transcriptome Dynamics in Response to β-Blockers
Authors: Tobin S. W. , Hashemi S. , Dadson K. , Turdi S. , Ebrahimian K. , Zhao J. , Sweeney G. , Grigull J. , McDermott J. C. 
Published: June 2017

AbstractMyocyte Enhancer Factor 2 (MEF2) mediates cardiac remodelling in heart failure (HF) and is also a target of β-adrenergic signalling, a front-line treatment for HF. We identified global gene transcription networks involved in HF with and without β-blocker treatment. Experimental HF by transverse aortic constriction (TAC) in a MEF2 “sensor” mouse model (6 weeks) was followed by four weeks of β-blockade with Atenolol (AT) or Solvent (Sol) treatment. Transcriptome analysis (RNA-seq) from left ventricular RNA samples and MEF2A depleted cardiomyocytes was performed. AT treatment resulted in an overall improvement in cardiac function of TAC mice and repression of MEF2 activity. RNA-seq identified 65 differentially expressed genes (DEGs) due to TAC treatment with enriched GO clusters including the inflammatory system, cell migration and apoptosis. These genes were mapped against DEGs in cardiomyocytes in which MEF2A expression was suppressed. Of the 65 TAC mediated DEGs, AT reversed the expression of 28 mRNAs. Rarres2 was identified as a novel MEF2 target gene that is upregulated with TAC in vivo and isoproterenol treatment in vitro which may have implications in cardiomyocyte apoptosis and hypertrophy. These studies identify a cohort of genes with vast potential for disease diagnosis and therapeutic intervention in heart failure.
Diagnostic accuracy of contrast enhanced ultrasound in patients with blunt abdominal trauma presenting to the emergency department: a systematic review and meta-analysis
Authors: Zhang Zhongheng , Hong Yucai , Liu Ning , Chen Yuhao 
Published: June 2017

AbstractWe aimed to investigate the diagnostic accuracy of contrast-enhanced ultrasound (CEUS) in evaluating blunt abdominal trauma for patients presenting to the emergency department. Electronic search of Scopus and Pubmed was performed from inception to September 2016. Human studies investigating the diagnostic accuracy of CEUS in identifying abdominal solid organ injuries were included. Risk of bias was assessed using the QUADAS tool. A total of 10 studies were included in the study and 9 of them were included for meta-analysis. The log(DOR) values ranged from 3.80 (95% CI: 2.81–4.79) to 8.52 (95% CI: 4.58–12.47) in component studies. The combined log(DOR) was 6.56 (95% CI: 5.66–7.45). The Cochran’s Q was 11.265 (p = 0.793 with 16 degrees of freedom), and the Higgins’ I2 was 0%. The CEUS had a sensitivity of 0.981 (95% CI: 0.868–0.950) and a false positive rate of 0.018 (95% CI: 0.010–0.032) for identifying parenchymal injuries, with an AUC of 0.984. CEUS performed at emergency department had good diagnostic accuracy in identifying abdominal solid organ injuries. CEUS can be recommended in monitoring solid organ injuries, especially for patients managed with non-operative strategy.
Association of plasma C-reactive protein level with the prevalence of colorectal adenoma: the Colorectal Adenoma Study in Tokyo
Authors: Kigawa Nano , Budhathoki Sanjeev , Yamaji Taiki , Iwasaki Motoki , Inoue Manami , Tsugane Shoichiro 
Published: June 2017

AbstractEpidemiologic studies have identified a positive association between obesity and colorectal neoplasia. Adiposity induces systemic low-grade inflammation, which is commonly assessed with a sensitive biomarker, C-reactive protein (CRP). To understand the molecular mechanisms of obesity in the etiology of colorectal neoplasia, the present study was conducted in 782 adenoma cases and 738 controls who underwent total colonoscopy, and their plasma CRP level was evaluated in relation to colorectal adenoma prevalence. A logistic regression model was used to compute odds ratios (OR) and 95% confidence intervals (CI) of adenoma according to quartile of plasma CRP. Plasma CRP level was positively associated with higher adenoma prevalence in all subjects (OR 1.30; 95% CI 0.94–1.79 for the highest versus lowest quartile; P trend = 0.031). Further analysis by adenoma size and number revealed a pronounced association with a larger size (≥5 mm) and multiple numbers (≥2). These positive associations were reduced to non-significance following further adjustment for body mass index, and OR for the highest versus lowest quartile of plasma CRP became 1.12 (95% CI 0.80–1.56; P trend = 0.25) in all subjects. In conclusion, this study suggests that obesity-related systemic low-grade inflammation may play an important role in the early stages of colorectal carcinogenesis.
Oxidative Stress Challenge Uncovers Trichloroacetaldehyde Hydrate-Induced Mitoplasticity in Autistic and Control Lymphoblastoid Cell Lines
Authors: Frye Richard Eugene , Rose Shannon , Wynne Rebecca , Bennuri Sirish C. , Blossom Sarah , Gilbert Kathleen M. , Heilbrun Lynne , Palmer Raymond F. 
Published: June 2017

AbstractMitoplasticity occurs when mitochondria adapt to tolerate stressors. Previously we hypothesized that a subset of lymphoblastoid cell lines (LCLs) from children with autistic disorder (AD) show mitoplasticity (AD-A), presumably due to previous environmental exposures; another subset of AD LCLs demonstrated normal mitochondrial activity (AD-N). To better understand mitoplasticity in the AD-A LCLs we examined changes in mitochondrial function using the Seahorse XF96 analyzer in AD and Control LCLs after exposure to trichloroacetaldehyde hydrate (TCAH), an in vivo metabolite of the environmental toxicant and common environmental pollutant trichloroethylene. To better understand the role of reactive oxygen species (ROS) in mitoplasticity, TCAH exposure was followed by acute exposure to 2,3-dimethoxy-1,4-napthoquinone (DMNQ), an agent that increases ROS. TCAH exposure by itself resulted in a decline in mitochondrial respiration in all LCL groups. This effect was mitigated when TCAH was followed by acute DMNQ exposure but this varied across LCL groups. DMNQ did not affect AD-N LCLs, while it neutralized the detrimental effect of TCAH in Control LCLs and resulted in a increase in mitochondrial respiration in AD-A LCLs. These data suggest that acute increases in ROS can activate mitochondrial protective pathways and that AD-A LCLs are better able to activate these protective pathways.
An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle
Authors: Kowsar Rasoul , Keshtegar Behrooz , Marey Mohamed. A. , Miyamoto Akio 
Published: June 2017

AbstractAfter intercourse/insemination, large numbers of sperm are deposited in the female reproductive tract (FRT), triggering a massive recruitment of neutrophils (PMNs) into the FRT, possibly to eliminate excessive sperm via phagocytosis. Some bovine oviductal fluid components (BOFCs) have been shown to regulate in vitro sperm phagocytosis (spermophagy) by PMNs. The modeling approach-based logistic regression (LR) and autoregressive logistic regression (ALR) can be used to predict the behavior of complex biological systems. We, first, compared the LR and ALR models using in vitro data to find which of them provides a better prediction of in vitro spermophagy in bovine. Then, the best model was used to identify and classify the reciprocal effects of BOFCs in regulating spermophagy. The ALR model was calibrated using an iterative procedure with a dynamical search direction. The superoxide production data were used to illustrate the accuracy in validating logit model-based ALR and LR. The ALR model was more accurate than the LR model. Based on in vitro data, the ALR predicted that the regulation of spermophagy by PMNs in bovine oviduct is more sensitive to alpha-1 acid glycoprotein (AGP), PGE2, bovine serum albumin (BSA), and to the combination of AGP or BSA with other BOFCs.
Clues on the origin of post-2000 earthquakes at Campi Flegrei caldera (Italy)
Authors: Chiodini G. , Selva J. , Del Pezzo E. , Marsan D. , De Siena L. , D’Auria L. , Bianco F. , Caliro S. , De Martino P. , Ricciolino P. , Petrillo Z. 
Published: June 2017

AbstractThe inter-arrival times of the post 2000 seismicity at Campi Flegrei caldera are statistically distributed into different populations. The low inter-arrival times population represents swarm events, while the high inter-arrival times population marks background seismicity. Here, we show that the background seismicity is increasing at the same rate of (1) the ground uplift and (2) the concentration of the fumarolic gas specie more sensitive to temperature. The seismic temporal increase is strongly correlated with the results of recent simulations, modelling injection of magmatic fluids in the Campi Flegrei hydrothermal system. These concurrent variations point to a unique process of temperature-pressure increase of the hydrothermal system controlling geophysical and geochemical signals at the caldera. Our results thus show that the occurrence of background seismicity is an excellent parameter to monitor the current unrest of the caldera.
Novel Interventional Management of Hepatic Hydatid Cyst with Nanosecond Pulses on Experimental Mouse Model
Authors: Chen Xinhua , Zhang Ruiqing , Aji Tuerganaili , Shao Yingmei , Chen Yonggang , Wen Hao 
Published: June 2017

AbstractThe nanosecond pulsed electric field (nsPEF) is investigated as an alternative plan for benign hepatic hydatid cyst. Altogether 72 C57B6 mice were included. Normal group (n = 12) had no parasite injection and the other 60 mice were used to induce hydatid cyst in liver by injecting protoscolices in portal vein. The liver hydatid cysts were exposed to nsPEF with different doses and then follow up. The standard surgery was performed as positive control. The hydatid cyst growth was monitored by ultrasound; the morphology was checked by gross anatomy and pathology was tested by H&E stain. In nsPEF-treated groups no hepatic failure nor bleeding were observed. As a comparison, in the surgery group, high post-treatment complications occurred (50%). Significant parasite growth inhibition was seen in high nsPEF dose group as compared with control group (P < 0.05). Pathological analysis confirmed destruction of hydatid cyst with sharp demarcation defined by the electrodes. Laboratory analysis showed nsPEF stimulated a time-dependent infection and recoverable liver function. The traumatic reactions defined by white blood count was significant lower than surgery groups (P < 0.05).Preliminary studies demonstrate nsPEF ablation can be applied on hepatic hydatid by inhibiting parasite growth, destructing the cyst and stimulating infections.
Charge tunable thin-film composite membranes by gamma-ray triggered surface polymerization
Authors: Reis Rackel , Duke Mikel C. , Tardy Blaise L. , Oldfield Daniel , Dagastine Raymond R. , Orbell John D. , Dumée Ludovic F. 
Published: June 2017

AbstractThin-film composite poly(amide) (PA) membranes have greatly diversified water supplies and food products. However, users would benefit from a control of the electrostatic interactions between the liquid and the net surface charge interface in order to benefit wider application. The ionic selectivity of the 100 nm PA semi-permeable layer is significantly affected by the pH of the solution. In this work, for the first time, a convenient route is presented to configure the surface charge of PA membranes by gamma ray induced surface grafting. This rapid and up-scalable method offers a versatile route for surface grafting by adjusting the irradiation total dose and the monomer concentration. Specifically, thin coatings obtained at low irradiation doses between 1 and 10 kGy and at low monomer concentration of 1 v/v% in methanol/water (1:1) solutions, dramatically altered the net surface charge of the pristine membranes from −25 mV to +45 mV, whilst the isoelectric point of the materials shifted from pH 3 to pH 7. This modification resulted in an improved water flux by over 55%, from 45.9 to up 70 L.m−2.h−1, whilst NaCl rejection was found to drop by only 1% compared to pristine membranes.
Cytotaxonomy and molecular phylogeny of the genus Cerapanorpa Gao, Ma & Hua, 2016 (Mecoptera: Panorpidae)
Authors: Miao Ying , Ma Na , Hua Bao-Zhen 
Published: June 2017

AbstractThe species of the genus Cerapanorpa Gao, Ma & Hua, 2016 (Mecoptera: Panorpidae) are characterized mainly by the presence of a finger-like anal horn on tergum VI of males and are distributed in the Oriental and eastern Palearctic regions. Herein, we investigated the pachytene banding patterns and reconstructed the Bayesian time-calibrated tree of some species of Cerapanorpa. All species examined display achiasmate meiosis and the same meiformula 2n = 42 + X0, reconfirming the monophyly of Cerapanorpa. The great variations in the size and number of heterochromatic bands suggest that they are reliable traits for species delimitation in Cerapanorpa. The existence of natural C-banding polymorphism indicates that chromosomal rearrangements likely have contributed to the diversification of chromosomal bands in Cerapanorpa. The closely related species of Cerapanorpa are reconfirmed to be evolutionarily independent entities by cytogenetic and molecular data. The divergence time estimated from the BEAST analysis shows that Cerapanorpa likely originated in the period from the Rupelian (30.7 Ma) to the Burdigalian (19.9 Ma), and most diversification occurred from the Burdigalian to the Piacenzian (17.4–2.8 Ma) in the Neogene. Our data suggest that chromosome rearrangements likely play a significant role in the speciation of Cerapanorpa.
Recent Advances in the Carbon–Carbon Bond-Forming Reactions of N-Acylketimines
Authors: Asahara Haruyasu 
Published: June 2017

N-Acylketimines are important synthetic building blocks used in the synthesis of a wide variety of functional amino compounds including cyclic and acyclic products. This review focuses on carbon–carbon bond-forming reactions of N-acylketimines to construct amino compounds having tetrasubstituted carbon centers.1	Introduction2	Preparation of N-Acylketimines2.1	Nucleophilic Addition of Amide Derivatives2.2	Acylation of Imine Derivatives2.3	An Aza-Wittig-Type Reaction2.4	Oxidation of Amine Precursors3	Reactions of N-Acylketimines3.1	Cyclic Ketimines3.2	Acyclic Ketimines4	Conclusions
IgE und Anti-IgE bei Asthma: eine wechselvolle Geschichte
Authors: Lommatzsch M. , Geißler K. , Bergmann K.-C. , Virchow J. 
Published: June 2017

ZusammenfassungEs war ein langer und verschlungener Weg, der zur Entdeckung von Immunglobulin E (IgE) in den Jahren 1966 und 1967 führte. Wir befinden uns aktuell auf einem langen und verschlungenen Weg, um die immunologische Basis der klinischen Wirkung des Anti-IgE-Antikörpers Omalizumab bei Asthma zu begreifen. Möglicherweise profitieren beim Asthma (ähnlich wie bei der chronisch spontanen Urtikaria) Patienten auf unterschiedliche immunologische Art und Weise von einer Omalizumab-Therapie. Dieser Artikel gibt einen Überblick über die Historie der IgE-Entdeckung und die aktuellen Konzepte der Anti-IgE-Therapie bei Asthma.
Epilepsiechirurgie bei Kindern und Jugendlichen
Authors: Ramantani Georgia , Zentner Josef 
Published: June 2017

ZusammenfassungDie Epilepsiechirurgie hat sich in den letzten Jahren als eine besonders wirksame Behandlungsoption bei Kindern und Jugendlichen mit pharmakoresistenten strukturellen Epilepsien etabliert. Dank der fortschreitenden Entwicklungen in der Neurochirurgie, Anästhesie und Intensivmedizin sind epilepsiechirurgische Eingriffe auch bei Säuglingen möglich und können hervorragende Ergebnisse liefern, vergleichbar mit den Ergebnissen bei Kindern und Jugendlichen. Spätestens bei nachgewiesener Pharmakoresistenz sollte bei Kindern mit strukturellen Epilepsien die Möglichkeit eines epilepsiechirurgischen Eingriffes erwogen und die entsprechende prächirurgische Diagnostik in einem Epilepsiezentrum mit pädiatrischer Expertise eingeleitet werden. Fokale kortikale Dysplasien und glioneuronale Tumore sind die häufigsten Ätiologien bei pädiatrischen Patienten, die epilepsiechirurgisch versorgt werden; in beiden Fällen hängt die postoperative Anfallsfreiheit von der Vollständigkeit der Resektion ab. Im Kindesalter dominieren die multilobären und hemisphärischen Eingriffe, im Gegensatz zu den Erwachsenenkohorten mit vorwiegend resektiven Eingriffen im Bereich des Temporallappens und nur wenigen extratemporalen und hemisphärischen Eingriffen. Die Ausdehnung der Eingriffe nimmt mit dem Alter der epilepsiechirurgischen Kandidaten ab. Kleinere Kinder benötigen oft größere Resektionen, können jedoch mögliche neurologische Defizite aufgrund der funktionellen Plastizität in der Regel gut kompensieren. Die postoperative Anfallsfreiheit hängt vom Epilepsiesyndrom, der zugrunde liegenden Ätiologie und der Abgrenzbarkeit des epileptogenen Areals ab. Zwei Drittel der Kinder bleiben im Langzeitverlauf nach einem epilepsiechirurgischen Eingriff anfallsfrei. Mit der Anfallskontrolle sind oftmals wesentliche Verbesserungen der Entwicklungsverläufe zu beobachten. Neben der Weiterentwicklung nicht-invasiver Methoden in der prächirurgischen Epilepsiediagnostik ist höchste Priorität, das Intervall zwischen Feststellung der Pharmakoresistenz, prächirurgischer Abklärung und epilepsiechirurgischer Behandlung bei geeigneten Kandidaten zu verkürzen. Multizentrische Studien mit längeren Beobachtungsintervallen sind dringend erforderlich, um die Prädiktoren der Anfallsfreiheit und der positiven psychomotorischen Entwicklung der Kinder zu identifizieren und eine verbesserte Kandidatenauswahl und Beratung der Patienten und deren Familien zu ermöglichen.