bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequences of Three
Actinobacteria
Strains Presenting New Candidate Organisms with High Potentials for Specific P450 Cytochromes
Authors: Grumaz Christian , Vainshtein Yevhen , Kirstahler Philipp , Luetz Stephan , Kittelmann Matthias , Schroer Kirsten , Eggimann Fabian K. , Czaja Rico , Vogel Andreas , Hilberath Thomas , Worsch Anne , Girhard Marco , Urlacher Vlada B. , Sandberg Marcel , Sohn Kai 
Published: July 2017

ABSTRACT

The three
Actinobacteria
strains
Streptomyces platensis
DSM 40041,
Pseudonocardia autotrophica
DSM 535, and
Streptomyces fradiae
DSM 40063 were described to selectively oxyfunctionalize several drugs. Here, we present their draft genomes to unravel their gene sets encoding promising cytochrome P450 monooxygenases associated with the generation of drug metabolites.
Genome Sequence of
Micromonospora
sp. NBS 11-29, an Antibiotic and Hydrolytic Enzyme Producer, Isolated from River Sediment in Brazil
Authors: Ichiwaki Simone , Costa Aline C. M. M. , Silva Eliane G. , Rada Lina R. M. , Lima Felipe R. , Ortíz-Vera Mabel P. , Garrido Leandro M. , Sato Maria Inês Z. , Araújo Welington L. , Padilla Gabriel 
Published: July 2017

ABSTRACT

The genus
Micromonospora
comprises actinomycetes with high biotechnological potential, due to their ability to produce secondary metabolites and enzymes. In this study, we report the draft genome sequence of
Micromonospora
sp. NBS 11-29, which showed antibacterial, cellulolytic, and xylanolytic activities under
in vitro
conditions.
De Novo
Whole-Genome Sequencing of the Wood Rot Fungus
Polyporus brumalis
, Which Exhibits Potential Terpenoid Metabolism
Authors: Lee Su-Yeon , An Ji-eun , Ryu Sun-Hwa , Kim Myungkil 
Published: July 2017

ABSTRACT

Polyporus brumalis
is able to synthesize several sesquiterpenes during fungal growth. Using a single-molecule real-time sequencing platform, we present the 53-Mb draft genome of
P. brumalis
, which contains 6,231 protein-coding genes. Gene annotation and isolation support genetic information, which can increase the understanding of sesquiterpene metabolism in
P. brumalis
.
Complete Genome Sequences of Three Xanthomonas citri Strains from Texas
Authors: Munoz Bodnar Alejandra , Santillana Gem , Mavrodieva Vessela , Liu Zhaowei , Nakhla Mark , Gabriel Dean W. 
Published: July 2017

ABSTRACT

The complete genome sequences of three
Xanthomonas citri
strains isolated from lime trees in Texas were found to belong to the A
w
group. All carried nearly identical large plasmids with similarity to those of a citrus canker strain from India and to xanthomonads from Africa and Colombia. All three strains harbored unusual
pthA
homologs.
Draft Genome Sequence of
Plasmodium gonderi
, a Malaria Parasite of African Old World Monkeys
Authors: Honma Hajime , Kawai Satoru , Motooka Daisuke , Nakamura Shota , Tougan Takahiro , Horii Toshihiro , Arisue Nobuko 
Published: July 2017

ABSTRACT

Plasmodium gonderi
is a primate parasite whose natural host is the African Old World monkeys. Here, we report the draft genome sequence for
P. gonderi
. The data are useful not only for understanding the evolution of malaria but also for allowing the comparative genomics of malaria parasites.
Draft Genome Sequences of Two Mycobacterium bovis Strains Isolated from Beef Cattle in Paraguay
Authors: Sanabria Lidia , Lagrave Lorena , Nishibe Christiane , Ribas Augusto C. A. , Zumárraga Martín J. , Almeida Nalvo F. , Araújo Flábio R. 
Published: July 2017

ABSTRACT

This work reports the draft genome sequences of the
Mycobacterium bovis
strains M1009 and M1010, isolated from the lymph nodes of two infected cows on a beef farm in Paraguay. Comparative genomics between these strains and other regional strains may provide more insights regarding
M. bovis
epidemiology in South America.
Draft Genome Sequences of Pseudoalteromonas tetraodonis CSB01KR and Pseudoalteromonas lipolytica CSB02KR, Isolated from the Gut of the Sea Cucumber
Apostichopus japonicus
Authors: Jo Jihoon , Choi Hyunjun , Lee Sung-Gwon , Oh Jooseong , Lee Hyun-Gwan , Park Chungoo 
Published: July 2017

ABSTRACT

We present here the complete genome sequences of two newly isolated
Pseudoalteromonas tetraodonis
and
Pseudoalteromonas lipolytica
strains, isolated from the gut of the sea cucumber
Apostichopus japonicus
, to provide a useful means for facilitating the study of antibacterial, bacteriolytic, agarolytic, and algicidal activities of marine
Pseudoalteromonas
species.
Complete Genome Sequences of Lactococcus lactis subsp.
lactis
bv. diacetylactis FM03 and Leuconostoc mesenteroides FM06 Isolated from Cheese
Authors: van Mastrigt Oscar , Abee Tjakko , Smid Eddy J. 
Published: July 2017

ABSTRACT

Here, the genome sequences of
Lactococcus lactis
subsp.
lactis
bv. diacetylactis FM03 and
Leuconostoc mesenteroides
FM06, both isolated from cheese, are presented. FM03 and FM06 contain 7 and 3 plasmids, respectively, that carry genes encoding functions important for growth and survival in dairy fermentations.
Ruxolitinib treatment in an infant with JAK2+ polycythaemia vera-associated Budd-Chiari syndrome
Authors: Coskun Mehmet Enes , Height Sue , Dhawan Anil , Hadzic Nedim 
Published: July 2017

Budd-Chiari syndrome (BCS) is caused by hepatic venous outflow obstruction commonly seen with myeloproliferative neoplasms (MPNs). Polycythaemia vera (PV) is a very rare MPN in childhood. This is the youngest reported patient diagnosed with PV and BCS secondary toJAK V617Fmutation.A 26-month-old girl was admitted with a 5-month history of abdominal distension, hepatosplenomegaly and ascites. Imaging studies revealed occlusion of the right hepatic vein and marked attenuation of the middle and left hepatic veins. BCS was diagnosed after excluding other causes of chronic liver disease. Mandatory prothrombotic workup revealed underlying PV.Partial recanalisation of hepatic veins occurred following anticoagulation therapy and PV was well controlled by pegylated interferon and hydroxycarbamide until she developed nephrotic syndrome, likely secondary to pegylated interferon. Therefore, treatment was modified to ruxolitinib, a novel-JAK-2 inhibitor; the therapy has been effective for almost 20 months with a good response and has no side effects.
Rationale and protocol for the After Diabetes Diagnosis REsearch Support System (ADDRESS): an incident and high risk type 1 diabetes UK cohort study
Authors: Walkey Helen C , Kaur Akaal , Bravis Vassiliki , Godsland Ian F , Misra Shivani , Williams Alistair J K , Bingley Polly J , Dunger David B , Oliver Nick , Johnston Desmond G 
Published: July 2017

IntroductionType 1 diabetes is heterogeneous in its presentation and progression. Variations in clinical presentation between children and adults, and with ethnic group warrant further study in the UK to improve understanding of this heterogeneity. Early interventions to limit beta cell damage in type 1 diabetes are undergoing evaluation, but recruitment is challenging. The protocol presented describes recruitment of people with clinician-assigned, new-onset type 1 diabetes to understand the variation in their manner of clinical presentation, to facilitate recruitment into intervention studies and to create an open-access resource of data and biological samples for future type 1 diabetes research.Methods and analysisUsing the National Institute for Health Research Clinical Research Network, patients >5 years of age diagnosed clinically with type 1 diabetes (and their siblings) are recruited within 6 months of diagnosis. Participants agree to have their clinical, laboratory and demographic data stored on a secure database, for their clinical progress to be monitored using information held by NHS Digital, and to be contacted about additional research, in particular immunotherapy and other interventions. An optional blood sample is taken for islet autoantibody measurement and storage of blood and DNA for future analyses. Data will be analysed statistically to describe the presentation of incident type 1 diabetes in a contemporary UK population.Ethics and disseminationEthical approval was obtained from the independent NHS Research Ethics Service. Results will be presented at national and international meetings and submitted for publication to peer-reviewed journals.
Evaluating the importance of policy amenable factors in explaining influenza vaccination: a cross-sectional multinational study
Authors: Wheelock Ana , Miraldo Marisa , Thomson Angus , Vincent Charles , Sevdalis Nick 
Published: July 2017

ObjectivesDespite continuous efforts to improve influenza vaccination coverage, uptake among high-risk groups remains suboptimal. We aimed to identify policy amenable factors associated with vaccination and to measure their importance in order to assist in the monitoring of vaccination sentiment and the design of communication strategies and interventions to improve vaccination rates.SettingThe USA, the UK and France.ParticipantsA total of 2412 participants were surveyed across the three countries.Outcome measuresSelf-reported influenza vaccination.MethodsBetween March and April 2014, a stratified random sampling strategy was employed with the aim of obtaining nationally representative samples in the USA, the UK and France through online databases and random-digit dialling. Participants were asked about vaccination practices, perceptions and feelings. Multivariable logistic regression was used to identify factors associated with past influenza vaccination.ResultsThe models were able to explain 64%–80% of the variance in vaccination behaviour. Overall, sociopsychological variables, which are inherently amenable to policy, were better at explaining past vaccination behaviour than demographic, socioeconomic and health variables. Explanatory variables included social influence (physician), influenza and vaccine risk perceptions and traumatic childhood experiences.ConclusionsOur results indicate that evidence-based sociopsychological items should be considered for inclusion into national immunisation surveys to gauge the public’s views, identify emerging concerns and thus proactively and opportunely address potential barriers and harness vaccination drivers.
Ventilatory function as a predictor of mortality in lifelong non-smokers: evidence from large British cohort studies
Authors: Gupta Ramyani P , Strachan David P 
Published: July 2017

BackgroundReduced ventilatory function is an established predictor of all-cause mortality in general population cohorts. We sought to verify this in lifelong non-smokers, among whom confounding by active smoking can be excluded, and investigate associations with circulatory and cancer deaths.MethodsIn UK Biobank, among 149 343 white never-smokers aged 40–69 years at entry, 2401 deaths occurred over a mean of 6.5-year follow-up. In the Health Surveys for England (HSE) 1995, 1996, 2001 and Scottish Health Surveys (SHS) 1998 and 2003 combined, there were 500 deaths among 6579 white never-smokers aged 40–69 years at entry, followed for a mean of 13.9 years. SD (z) scores for forced expiratory volume in the first second (FEV1) and forced vital capacity (FVC) were derived using Global Lung Initiative 2012 reference equations. These z-scores were related to deaths from all causes, circulatory disease and cancers using proportional hazards models adjusted for age, sex, height, socioeconomic status, region and survey.ResultsIn the HSE–SHS data set, decreasing z-scores for FEV1 (zFEV1) and FVC (zFVC) were each associated to a similar degree with increased all-cause mortality (hazard ratios per unit decrement 1.17, 95% CI 1.09 to 1.25 for zFEV1 and 1.19, 95% CI 1.10 to 1.28 for zFVC). This was replicated in Biobank (HRs 1.21, 95% CI 1.17 to 1.26 and 1.24, 1.19 to 1.29, respectively). zFEV1 and zFVC were less strongly associated with mortality from circulatory diseases in HSE–SHS (HR 1.22, 95% CI 1.06 to 1.40 for zFVC) than in Biobank (HR 1.47, 95% CI 1.35 to 1.60 for zFVC). For cancer mortality, HRs were more consistent between cohorts (for zFVC: HRs 1.12, 95% CI 1.01 to 1.24 in HSE–SHS and 1.10, 1.05 to 1.15 in Biobank). The strongest associations were with respiratory mortality (for zFVC: HRs 1.61, 95% CI 1.25 to 2.08 in HSE–SHS and 2.15, 1.77 to 2.61 in Biobank).ConclusionsSpirometric indices predicted mortality more strongly than systolic blood pressure or body mass index, emphasising the importance of promoting lung health in the general population, even among lifelong non-smokers.
Cross-sectional study of the hospital management of adult patients with a suspected seizure (EPIC2)
Authors: Dickson Jon Mark , Dudhill Hannah , Shewan Jane , Mason Sue , Grünewald Richard A , Reuber Markus 
Published: July 2017

ObjectiveTo determine the clinical characteristics, management and outcomes of patients taken to hospital by emergency ambulance after a suspected seizure.DesignQuantitative cross-sectional retrospective study of a consecutive series of patients.SettingAn acute hospital trust in a large city in England.ParticipantsIn 2012–2013, the regions’ ambulance service managed 605 481 emergency incidents, 74 141/605 481 originated from Sheffield (a large city in the region), 2121/74 141 (2.9%) were suspected seizures and 178/2121 occurred in May 2012. We undertook detailed analysis of the medical records of the 91/178 patients who were transported to the city's acute hospital. After undertaking a retrospective review of the medical records, the best available aetiological explanation for the seizures was determined.ResultsThe best available aetiological explanation for 74.7% (68/91) of the incidents was an epileptic seizure, 11.0% (10/91) were psychogenic non-epileptic seizures and 9.9% (9/91) were cardiogenic events. The epileptic seizures fall into the following four categories: first epileptic seizure (13.2%, 12/91), epileptic seizure with a historical diagnosis of epilepsy (30.8%, 28/91), recurrent epileptic seizures without a historical diagnosis of epilepsy (20.9%, 19/91) and acute symptomatic seizures (9.9%, 9/91). Of those with seizures (excluding cardiogenic events), 2.4% (2/82) of patients were seizing on arrival in the Emergency Department (ED), 19.5% (16/82) were postictal and 69.5% (57/82) were alert. 63.4% (52/82) were discharged at the end of their ED attendance and 36.5% (19/52) of these had no referral or follow-up.ConclusionsMost suspected seizures are epileptic seizures but this is a diagnostically heterogeneous group. Only a small minority of patients require emergency medical care but most are transported to hospital. Few patients receive expert review and many are discharged home without referral to a specialist leaving them at risk of further seizures and the associated morbidity, mortality and health services costs of poorly controlled epilepsy.
Low-value clinical practices in injury care: a scoping review protocol
Authors: Moore Lynne , Boukar Khadidja Malloum , Tardif Pier-Alexandre , Stelfox Henry T , Champion Howard , Cameron Peter , Gabbe Belinda , Yanchar Natalie , kortbeek John , Lauzier François , Légaré France , Archambault Patrick , Turgeon Alexis F 
Published: July 2017

IntroductionPreventable injuries lead to 200 000 hospital stays, 60 000 disabilities, and 13 000 deaths per year in Canada with direct costs of $20 billion. Overall, potentially unnecessary medical interventions are estimated to consume up to 30% of healthcare resources and may expose patients to avoidable harm. However, little is known about overuse for acute injury care. We aim to identify low-value clinical practices in injury care.Methods and analysisWe will perform a scoping review of peer-reviewed and non-peer-reviewed literature to identify research articles, reviews, recommendations and guidelines that identify at least one low-value clinical practice specific to injury populations. We will search Medline, EMBASE, COCHRANE central, and BIOSIS/Web of Knowledge databases, websites of government agencies, professional societies and patient advocacy organisations, thesis holdings and conference proceedings. Pairs of independent reviewers will evaluate studies for eligibility and extract data from included articles using a prepiloted and standardised electronic data abstraction form. Low-value clinical practices will be categorised using an extension of the Agency for Healthcare Research and Quality conceptual framework and data will be presented using narrative synthesis.Ethics and disseminationEthics approval is not required as original data will not be collected. This study will be disseminated in a peer-reviewed journal, international scientific meetings, and to knowledge users through clinical and healthcare quality associations. This review will contribute new knowledge on low-value clinical practices in acute injury care. Our results will support the development indicators to measure resource overuse and inform policy makers on potential targets for deadoption in injury care.
Mechanical thrombectomy of M1 and M2 middle cerebral artery occlusions
Authors: Salahuddin Hisham , Ramaiah Guru , Slawski Diana E , Shawver Julie , Buehler Mark , Zaidi Syed F , Jumaa Mouhammad 
Published: July 2017

BackgroundOver half of patients who receive intravenous tissue plasminogen activator for middle cerebral artery division (MCA-M2) occlusion do not recanalize, leaving a large percentage of patients who may need mechanical thrombectomy (MT). However, the outcomes of MT for M2 occlusion have not been well characterized.ObjectiveTo determine if MT of M2 occlusion is as safe and efficacious as current standard-of-care MT for M1 occlusions.MethodsWith institutional review board approval, we retrospectively reviewed records of 212 patients undergoing MT for isolated MCA M1 or M2 occlusions during a 36-month period (Sept 2013 to Sept 2016) at two centres. Treatment variables, clinical outcomes, and complications in each group were recorded.ResultsThere were 153 M1 MCA occlusions and 59 M2 MCA occlusions. No statistically significant difference was found in the rate of mortality (20% in M1 vs 13.6% in M2, p=0.32), excellent (34.5% vs 37.3%, p=0.75) or good (51% vs 55.9%, p=0.54) clinical outcomes between the two groups. Infarct volumes (48.4 mL vs 46.2 mL, p=0.62) were comparable between the two groups, as were the rates of hemorrhagic (3.3% vs 3.4%, p=1.0) and procedural complications (3.3% vs 5.1%, p=0.69).ConclusionOur data on MT targeting M2 occlusions demonstrates reasonable safety and functional outcomes. Further randomized clinical trials are needed to clarify which patients may benefit from MT for M2 occlusions.
Loss of nitric oxide-mediated inhibition of purine neurotransmitter release in the colon in the absence of interstitial cells of Cajal
Authors: Durnin Leonie , Lees Andrea , Manzoor Sheerien , Sasse Kent C. , Sanders Kenton M. , Mutafova-Yambolieva Violeta N. 
Published: July 2017

Regulation of colonic motility depends on the integrity of enteric inhibitory neurotransmission mediated by nitric oxide (NO), purine neurotransmitters, and neuropeptides. Intramuscular interstitial cells of Cajal (ICC-IM) and platelet-derived growth factor receptor-α-positive (PDGFRα+) cells are involved in generating responses to NO and purine neurotransmitters, respectively. Previous studies have suggested a decreased nitrergic and increased purinergic neurotransmission in KitW/KitW-v ( W/Wv) mice that display lesions in ICC-IM along the gastrointestinal tract. However, contributions of NO to these phenotypes have not been evaluated. We used small-chamber superfusion assays and HPLC to measure the spontaneous and electrical field stimulation (EFS)-evoked release of nicotinamide adenine dinucleotide (NAD+)/ADP-ribose, uridine adenosine tetraphosphate (Up4A), adenosine 5′-triphosphate (ATP), and metabolites from the tunica muscularis of human, monkey, and murine colons and circular muscle of monkey colon, and we tested drugs that modulate NO levels or blocked NO receptors. NO inhibited EFS-evoked release of purines in the colon via presynaptic neuromodulation. Colons from W/Wv, Nos1−/−, and Prkg1−/− mice displayed augmented neural release of purines that was likely due to altered nitrergic neuromodulation. Colons from W/Wv mice demonstrated decreased nitrergic and increased purinergic relaxations in response to nerve stimulation. W/Wv mouse colons demonstrated reduced Nos1 expression and reduced NO release. Our results suggest that enhanced purinergic neurotransmission may compensate for the loss of nitrergic neurotransmission in muscles with partial loss of ICC. The interactions between nitrergic and purinergic neurotransmission in the colon provide novel insight into the role of neurotransmitters and effector cells in the neural regulation of gastrointestinal motility. NEW & NOTEWORTHY This is the first study investigating the role of nitric oxide (NO) and intramuscular interstitial cells of Cajal (ICC-IM) in modulating neural release of purines in colon. We found that NO inhibited release of purines in human, monkey, and murine colons and that colons from KitW/KitW-v ( W/Wv) mice, which present with partial loss of ICC-IM, demonstrated augmented neural release of purines. Interactions between nitrergic and purinergic neurotransmission may affect motility in disease conditions with ICC-IM deficiencies.
A comparison of passive hindlimb cycling and active upper-limb exercise provides new insights into systolic dysfunction after spinal cord injury
Authors: DeVeau Kathryn M. , Harman Kathryn A. , Squair Jordan W. , Krassioukov Andrei V. , Magnuson David S. K. , West Christopher R. 
Published: July 2017

Active upper-limb and passive lower-limb exercise are two interventions used in the spinal cord injury (SCI) population. Although the global cardiac responses have been previously studied, it is unclear how either exercise influences contractile cardiac function. Here, the cardiac contractile and volumetric responses to upper-limb (swim) and passive lower-limb exercise were investigated in rodents with a severe high-thoracic SCI. Animals were divided into control (CON), SCI no exercise (NO-EX), SCI passive hindlimb cycling (PHLC), or SCI swim (SWIM) groups. Severe contusion SCI was administered at the T2 level. PHLC and SWIM interventions began on day 8 postinjury and lasted 25 days. Echocardiography and dobutamine stress echocardiography were performed before and after injury. Cardiac contractile indexes were assessed in vivo at study termination via a left ventricular pressure-volume conductance catheter. Stroke volume was reduced after SCI (91 µl in the NO-EX group vs. 188 µl in the CON group, P < 0.05) and was reversed at study termination in the PHLC (167 µl) but not SWIM (90 µl) group. Rates of contraction were reduced in NO-EX versus CON groups (6,079 vs. 9,225 mmHg, respectively, P < 0.05) and were unchanged by PHLC and SWIM training. Similarly, end-systolic elastance was reduced in the NO-EX versus CON groups (0.67 vs. 1.37 mmHg/µl, respectively, P < 0.05) and was unchanged by PHLC or SWIM training. Dobutamine infusion normalized all pressure indexes in each SCI group (all P < 0.05). In conclusion, PHLC improves flow-derived cardiac indexes, whereas SWIM training displayed no cardiobeneficial effect. Pressure-derived deficits were corrected only with dobutamine, suggesting that reduced β-adrenergic stimulation is principally responsible for the impaired cardiac contractile function after SCI. NEW & NOTEWORTHY This is the first direct comparison between the cardiac changes elicited by active upper-limb or passive lower-limb exercise after spinal cord injury. Here, we demonstrate that lower-limb exercise positively influences flow-derived cardiac indexes, whereas upper-limb exercise does not. Furthermore, neither intervention corrects the cardiac contractile dysfunction associated with spinal cord injury.