bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing the therapeutic potential of Ganoderma lucidum spore oil in alleviating periodontal tissue damage in murine periodontitis model
Authors: Kim Ji-Hyun , Choi Weon-Young , Jeong Seung-Jun , Park Ka Hyon , Lee Gyuseok , Kim Mangeun , Joo Soo-Chang , Kim Seongjun , Cho Beom-Jin , Son Young-Ok , Ryu Je-Hwang 
Published: September 2024

AbstractPeriodontal disease presents a significant challenge in oral health due to its chronic inflammatory nature and subsequent degradation of tooth-supporting structures. Natural compounds have attracted attention for their potential therapeutic effects in alleviating symptoms of periodontitis (PD). In this study, we investigated the impact of Ganoderma lucidum spore oil (GLSO), a lipid component extracted from broken-walled GLS using the supercritical CO2 extraction method, on PD pathogenesis in vitro and in vivo. Treatment of human gingival fibroblasts with GLSO resulted in a significant reduction in the expression of inflammatory factors, including matrix metalloproteinase (MMP)-1 and interleukin (IL)-8, upregulated by lipopolysaccharide or IL-1β. Molecular mechanism studies revealed that the observed decrease in inflammatory factor expression may be attributed to the inhibition of phosphorylated c-Jun N-terminal kinase activity by GLSO. Furthermore, intraperitoneal injection of GLSO in a ligature-induced PD mouse model led to a notable reduction in periodontal inflammation and alveolar bone loss, accompanied by decreased levels of MMP-1 and IL-8. These in vivo results support the potential therapeutic efficacy of GLSO in alleviating PD symptoms. Overall, our study provides novel insights into the beneficial effects of GLSO in PD management. Further research is warranted to elucidate the underlying molecular mechanisms and explore the clinical applicability of GLSO as a promising therapeutic agent for PD treatment.
Networks as tools for defining emergent properties of microbiomes and their stability
Authors: Kajihara Kacie T. , Hynson Nicole A. 
Published: September 2024

AbstractThe potential promise of the microbiome to ameliorate a wide range of societal and ecological challenges, from disease prevention and treatment to the restoration of entire ecosystems, hinges not only on microbiome engineering but also on the stability of beneficial microbiomes. Yet the properties of microbiome stability remain elusive and challenging to discern due to the complexity of interactions and often intractable diversity within these communities of bacteria, archaea, fungi, and other microeukaryotes. Networks are powerful tools for the study of complex microbiomes, with the potential to elucidate structural patterns of stable communities and generate testable hypotheses for experimental validation. However, the implementation of these analyses introduces a cascade of dichotomies and decision trees due to the lack of consensus on best practices. Here, we provide a road map for network-based microbiome studies with an emphasis on discerning properties of stability. We identify important considerations for data preparation, network construction, and interpretation of network properties. We also highlight remaining limitations and outstanding needs for this field. This review also serves to clarify the varying schools of thought on the application of network theory for microbiome studies and to identify practices that enhance the reproducibility and validity of future work.
The performance of alternative GPS tracking devices: a case report on wild boars (Sus scrofa)
Authors: Kunnasranta Mervi , Miettinen Elmo , Melin Markus , Meller Anna , Väänänen Veli-Matti , Huitu Otso , Holmala Katja 
Published: September 2024

Abstract
Background
Telemetry studies on terrestrial mammals have been carried out almost exclusively using neck collar-mounted devices. However, collars are not suitable for all species and all age classes and may therefore compromise animal welfare and data quality requirements. Here, we evaluate the use of non-collar tracking devices on wild boars (Sus scrofa), a species for which collars may be problematic due to neck shape and seasonal body mass variation. We tested pelt-glued tags and three types of GPS ear tags. In addition, animals were marked with plain cattle ear tags.

Results
The tested GPS tags exhibited various technical challenges related mostly to satellite coverage of the study area and tag loss. However, GPS devices enabled moderate movement monitoring of weeks to months, whereas plain cattle ear tags offered information on displacement over longer time intervals. The longest direct dispersal distance (163 km) by a wild boar sounder was discovered by cattle tags.

Conclusions
Data volume and quality obtained from small tags are inferior to data provided by GPS collars, but low-fix rate tags may enable monitoring of individuals with reduced invasiveness. Moreover, these tags enable tracking in cases where the collar is not an option. The low price and small size of the devices together with technical developments may offer cost-effective tools for future studies on dispersal and survival.
Repurposing idle wells in the North German Basin as deep borehole heat exchangers
Authors: Koltzer Nora , Schoenherr Johannes , Sporleder Maximilian , Niederau Jan , Wellmann Florian 
Published: September 2024

AbstractThis study investigates the feasibility to repurpose wells from gas production for geothermal closed-loop application in the North German Basin (NGB). The objective for this research topic is to extend the value-added chain of idle wells by re-completion as coaxial deep borehole heat exchangers as an efficient way to produce green energy without drilling new wells by saving the carbon emission and costs of building a new geothermal well. With numerical models of two typical geological settings of the NGB and two different completion schemes, it is possible to simulate the thermal performance over a lifetime of 30 years. The calculated heat extraction rates range from 200 to 400 kW, with maximum values of up to 600 kW. Sensitivity analyses demonstrate that re-completion depth and injection temperature are the most sensitive parameters of thermal output determination. The heat demand around the boreholes is mapped, and heat generation costs are calculated with heating network simulations. The initial production costs for heat are comparable to other renewable energy resources like biomass and competitive against gas prices in 2022. This study highlights available geothermal resources’ environmental and economic potential in already installed wells. The application has almost no geological and no drilling risks and may be installed at any idle well location.
Association of corticosteroid therapy with reduced acute kidney injury and lower NET markers in severe COVID-19: an observational study
Authors: Bülow Anderberg Sara , Huckriede Joram , Hultström Michael , Larsson Anders , de Vries Femke , Lipcsey Miklos , Nicolaes Gerry A. F. , Frithiof Robert 
Published: September 2024

Abstract
Background
Acute kidney injury (AKI) is common in critical cases of coronavirus disease 2019 (COVID-19) and associated with worse outcome. Dysregulated neutrophil extracellular trap (NET) formation is one of several suggested pathophysiological mechanisms involved in the development of COVID-19 associated AKI. The corticosteroid dexamethasone was implemented as a standard treatment for severe COVID-19 as of June 2020. A sub-analysis of a prospective observational single center study was performed to evaluate the effect of corticosteroid treatment on AKI development and NET markers in critical cases of COVID-19.

Results
Two hundred and ten adult patients admitted to intensive care at a tertiary level hospital due to respiratory failure or shock secondary to SARS-CoV-2-infection between March 13th 2020 and January 14th 2021 were included in the study. Ninety-seven of those did not receive corticosteroids. One hundred and thirteen patients were treated with corticosteroids [dexamethasone (n = 98) or equivalent treatment (n = 15)], but the incidence of AKI was assessed only in patients that received corticosteroids before any registered renal dysfunction (n = 63). Corticosteroids were associated with a lower incidence of AKI (19% vs 55.8%, p < 0.001). Fewer patients demonstrated detectable concentrations of extracellular histones in plasma when treated with corticosteroids (8.7% vs 43.1%; p < 0.001). Extracellular histones and in particular non-proteolyzed histones were observed more frequently with increasing AKI severity (p < 0.001). MPO-DNA was found in lower concentrations in patients that received corticosteroids before established renal dysfunction (p = 0.03) and was found in higher concentrations in patients with AKI stage 3 (p = 0.03). Corticosteroids did not ameliorate established AKI during the first week of treatment.

Conclusion
Corticosteroid treatment in severe COVID-19 is associated with a lower incidence of AKI and reduced concentrations of NET markers in plasma.
Maternal stress during pregnancy alters circulating small extracellular vesicles and enhances their targeting to the placenta and fetus
Authors: Sánchez-Rubio Mario , Abarzúa-Catalán Lorena , del Valle Ana , Méndez-Ruette Maxs , Salazar Natalia , Sigala Jacinta , Sandoval Soledad , Godoy María Inés , Luarte Alejandro , Monteiro Lara J. , Romero Roberto , Choolani Mahesh A. , Wyneken Úrsula , Illanes Sebastián E. , Bátiz Luis Federico 
Published: September 2024

Abstract
Background
Maternal psychological distress during pregnancy can negatively impact fetal development, resulting in long-lasting consequences for the offspring. These effects show a sex bias. The mechanisms whereby prenatal stress induces functional and/or structural changes in the placental-fetal unit remain poorly understood. Maternal circulating small extracellular vesicles (sEVs) are good candidates to act as “stress signals” in mother-to-fetus communication. Using a repetitive restraint-based rat model of prenatal stress, we examined circulating maternal sEVs under stress conditions and tested whether they could target placental-fetal tissues.

Results
Our mild chronic maternal stress during pregnancy paradigm induced anhedonic-like behavior in pregnant dams and led to intrauterine growth restriction (IUGR), particularly in male fetuses and placentas. The concentration and cargo of maternal circulating sEVs changed under stress conditions. Specifically, there was a significant reduction in neuron-enriched proteins and a significant increase in astrocyte-enriched proteins in blood-borne sEVs from stressed dams. To study the effect of repetitive restraint stress on the biodistribution of maternal circulating sEVs in the fetoplacental unit, sEVs from pregnant dams exposed to stress or control protocol were labeled with DiR fluorescent die and injected into pregnant females previously exposed to control or stress protocol. Remarkably, maternal circulating sEVs target placental/fetal tissues and, under stress conditions, fetal tissues are more receptive to sEVs.

Conclusion
Our results suggest that maternal circulating sEVs can act as novel mediators/modulators of mother-to-fetus stress communication. Further studies are needed to identify placental/fetal cellular targets of maternal sEVs and characterize their contribution to stress-induced sex-specific placental and fetal changes.
Investigation of the optimal method of oxygen administration with simultaneous use of a surgical mask in postoperative patients: a randomized cross-over study
Authors: Kamiyama Aya , Takazawa Tomonori , Matsui Yusuke , Nagumo Kazuhiro , Arai Seiji , Saito Shigeru 
Published: September 2024

Abstract
Background
From the standpoint of infection prevention, anesthesiologists need to simultaneously use a surgical mask and an oxygen mask when administering oxygen to patients. However, there is a lack of scientific evidence to justify this method. We aimed to investigate a suitable method of oxygen administration when using a surgical mask in postoperative patients.

Methods
This was a randomized, single-blind, cross-over study involving 42 patients admitted to the ICU. We compared three methods of oxygen administration: nasal cannula under the surgical mask, oxygen mask under the surgical mask, and oxygen mask above the surgical mask, using a cross-over design. The primary endpoint was partial pressure of arterial oxygen (PaO2). The secondary endpoint was partial pressure of arterial carbon dioxide (PaCO2).

Results
PaO2 was higher when the oxygen mask was placed under the surgical mask (median values 197.7 mmHg), the nasal cannula was under the surgical mask (180.6 mmHg), and the oxygen mask was above the surgical mask (143.0 mmHg), in descending order, with significant differences between all groups (P < 0.001). PaCO2 did not differ between groups.

Conclusions
The current standard method of administering oxygen to postoperative patients using an oxygen mask over a surgical mask results in poor oxygenation. Adopting the method of oxygen administration under the surgical mask via an oxygen mask or nasal cannula should be considered instead.
Association between caffeine intake and erectile dysfunction: a meta-analysis of cohort studies
Authors: Karimi Mehdi , Asbaghi Omid , Kazemi Kimia , Sedgi Fatemeh Maleki , Soleimani Ensiye , Moghadam Hanieh Keikhay 
Published: September 2024

Abstract
Background
Erectile dysfunction (ED) is a common condition with various contributing factors, including lifestyle and dietary habits. Caffeine, a widely consumed stimulant, has been linked to multiple physiological effects on vascular function and hormonal balance that might influence sexual function. This meta-analysis aims to evaluate the association between caffeine intake and the risk of ED by analyzing data from cohort studies.

Methods
A systematic search was conducted across PubMed, Web of Science, Scopus, and Embase databases, and a manual search was conducted on Google Scholar for studies on the relationship between caffeine intake and ED in adult men. The search included observational studies published up to April 1, 2024. Four cohort studies were included, and their data were extracted and analyzed by STATA version 18.

Results
Four included cohort studies comprised 51,665 cohort members. The study population included adult males, on average, aged 18 to 80. The results indicate that there was no significant relationship between coffee consumption and the risk of ED (relative risk [RR] = 0.94, 95% CI: 0.86–1.03; p = 0.999).

Conclusions
The current evidence suggests no significant relationship between caffeine intake and ED, but limited studies limit conclusions. Future research should focus on larger sample sizes, standardized outcome assessments, and different dosages and forms of caffeine consumption.
A novel approach for breast cancer treatment: the multifaceted antitumor effects of rMeV-Hu191
Authors: Zheng Xiao-Yu , Lv Yao , Xu Ling-Yan , Zhou Dong-Ming , Yu Lan , Zhao Zheng-Yan 
Published: September 2024

Abstract
Background
The therapeutic potential of oncolytic measles virotherapy has been demonstrated across various malignancies. However, the effectiveness against human breast cancer (BC) and the underlying mechanisms of the recombinant measles virus vaccine strain Hu191 (rMeV-Hu191) remain unclear.

Methods
We utilized a range of methods, including cell viability assay, Western blot, flow cytometry, immunofluorescence, SA-β-gal staining, reverse transcription quantitative real-time PCR, transcriptome sequencing, BC xenograft mouse models, and immunohistochemistry to evaluate the antitumor efficacy of rMeV-Hu191 against BC and elucidate the underlying mechanism. Additionally, we employed transcriptomics and gene set enrichment analysis to analyze the lipid metabolism status of BC cells following rMeV-Hu191 infection.

Results
Our study revealed the multifaceted antitumor effects of rMeV-Hu191 against BC. rMeV-Hu191 induced apoptosis, inhibited proliferation, and promoted senescence in BC cells. Furthermore, rMeV-Hu191 was associated with changes in oxidative stress and lipid homeostasis in infected BC cells. In vivo, studies using a BC xenograft mouse model confirmed a significant reduction in tumor growth following local injection of rMeV-Hu191.

Conclusions
The findings highlight the potential of rMeV-Hu191 as a promising treatment for BC and provide valuable insights into the mechanisms underlying its oncolytic effect.
Linear measurements of the mandible on panoramic radiograph for sex estimation in populations in Yogyakarta, Indonesia
Authors: Datau Selcaria Istna , Aisha Sekar Khansa , Aulia Nurulita Fida , Shantiningsih Rurie Ratna , Mudjosemedi Munakhir , Yanuaryska Ryna Dwi , Widyaningrum Rini 
Published: September 2024

Abstract
Background
Identification is one of the main aspects of forensics. Sex estimation is an essential part of identification because it can simplify the whole process. Mandible is the largest, strongest, and sexually dimorphic bone and is part of the orofacial structure, which has the second highest level of dimorphism after the pelvic bone. Sex estimation using the mandible can be performed by conducting various linear measurements on a panoramic radiograph, including right minimum ramus width (RMiRW), left minimum ramus width (LMiRW), right projective height of ramus (RPHR), left projective height of ramus (LPHR), right maximum ramus width (RMxRW), left maximum ramus width (LMxRW), right coronoid height (RCH), left coronoid height (LCH), symphysis height (SH), right mandibular corpus height (RMCH), and left mandibular corpus height (LMCH). This study aimed to analyze how linear measurements in panoramic radiographs differ between men and women and to estimate sex by utilizing these parameters.

Results
In this study, 195 panoramic radiographs from 95 men and 100 women aged 20–40 years obtained from our dental hospital were used as a training data. Meanwhile, 61 panoramic radiographs from 29 men and 32 women outside the training data with same characteristics were used as the testing data. The linear measurements of the mandible using panoramic radiographs were taken with EzDent-i Vatech software. Independent t-test showed significant difference (p < 0.05) in some linear measurements of the mandible between adult men and women. These significantly different linear measurements were then subjected to discriminant function analysis to produce sex estimation equations. The equation accuracy percentage ranged between 63.6 and 94.4% for the training sample and 59.5% and 85% for the testing sample.

Conclusion
The linear measurements of the mandible using diagnostically acceptable panoramic radiographs taken from a patient with standardized head positioning can serve as an alternative method for sex estimation. The accuracy of discriminant analysis for sex estimation varies depending on the parameter used in the estimation.
Investigating alexithymia, empathy, and resilience in medical students during pandemic era: a cross-sectional study in northern Iran
Authors: Elyasi Forouzan , Amirsoleimani Yeganeh , Hamzehpour Romina , Moosazadeh Mahmood , Zarghami Mehran , Vajdi Maryam , Alamouti Elham Motevalli , Arimi Fatemeh Alizadeh 
Published: September 2024

Abstract
Background and aim
Alexithymia is defined as emotional response inhibition. As well, empathy refers to the ability to put oneself in someone’s position and resilience is the capacity to recover from a series of negative emotional experiences. Considering the psychological distress induced by the coronavirus disease 2019 (COVID-19) pandemic together with academic stress and the role of empathy in physician–patient relationships, the present study was to investigate alexithymia, empathy, and resilience in Iranian medical interns and residents.

Materials and methods
This cross-sectional study was fulfilled in northern Iran in 2021–2022. In total, 394 medical interns and residents were initially recruited for this purpose. Then, an online sociodemographic survey form (SDSF), the Jefferson Scale of Empathy (JSE), the Toronto Alexithymia Scale (TAS-20), and the Connor–Davidson Resilience Scale (CD-RISC) were completed. The data analysis was performed using the IBM SPSS Statistics (ver.26) software in regard to the p < 0.05 significance level.

Results
The mean age of the study participants was 28.8 ± 5.00. As well, 38.1% of these individuals were male, 62.2% of the cases were single, and 54.6% of them were medical interns. The mean value of empathy, resilience and alexithymia was 89.90 ± 14.00, 49.75 ± 10.56, and 46.40 ± 16.40, respectively. No significant relationship was found between empathy and educational level (p = 0.532). As well, medical interns empathy and resilience than residents (p = 0.000 & p = 0.000, respectively). Besides, male participants had more empathy and resilience (p = 0.000 & p = 0.007).

Conclusion
Low empathy and resilience in medical interns and residents, especially in women who make up the majority of them, can be a warning for health care in Iran.
Empowering sustainable hotels: a guest-centric optimization for vehicle-to-building integration
Authors: Valett Lynne , Bollenbach Jessica , Keller Robert 
Published: September 2024

AbstractIn light of global warming, hotels account for one of the highest energy demands within the building sector, offering great decarbonization potential. As electrification increases, so does the demand for electric vehicles (EVs) charging stations at hotels and the proportion of Vehicle-to-Building-capable EVs. Therefore, the study explores the potential of guest-centric energy management. To accomplish this, we develop an optimization model for an energy management system that focuses on either cost-efficiency or carbon dioxide equivalents (CO2)-efficiency, grounded in a real-world case study. Through scenario analyses considering seasons as well as different guest mobility behaviors, this study discusses the expenses associated with CO2 savings using digital solutions. It emphasizes the currently perceived conflict between cost reduction and decarbonization goals to achieve a sustainable design of information systems. Thereby, this study highlights the critical importance of individual mobility behavior in enabling sustainable energy management for hotels.