bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mid-term results of a case series on branched xenopericardial roll graft replacement to treat aortic arch/arch graft infection
Authors: Kubota Hiroshi , Endo Hidehito , Minegishi Sachito , Tsuchiya Hiroshi , Inaba Yusuke , Takahashi Yu , Shimasaki Teppei , Kurai Daisuke , Nakazawa Harumasa , Ohura Norihiko 
Published: October 2024

Abstract

OBJECTIVES
We conducted this case series to evaluate the feasibility and mid-term outcomes of orthotopic aortic arch reconstruction using xenopericardial grafts to treat native aortic arch/arch graft infection.

METHODS
Between 2010 and 2023, adopting a multidisciplinary approach, we treated consecutive patients with aortic arch/arch graft infection by orthotopic branched xenopericardial roll graft replacement and tissue filling. The end points of the study were the graft reinfection and graft-related complications such as pseudoaneurysm formation, thromboembolism, graft stenosis, graft calcification and death.

RESULTS
The subjects were 11 patients (8 men and 3 women) with a median age of 66 [51–79, (interquartile range) 59.0, 70.5] years. Of the 11 patients, 7 underwent preoperative mediastinal irrigation/negative-pressure wound treatment. The 30-day mortality was 0% (0/11 patients), and 1 patient died in the hospital after 8 months. Aorta-related death occurred in 18.2% (2/11 patients), with both cases developing local recurrence of fungal infection. The remaining 9 patients (81.8%) remained free of reinfection. The estimated 1-, 3-, 5- and 7-year risk-adjusted survival rates were 91 ± 9%, 81 ± 12%, 58 ± 16% and 58 ± 16%, respectively. The estimated 1-, 3-, 5- and 7-year cumulative aorta-related mortality rates were 9 ± 9%, 19 ± 12%, 19 ± 12% and 19 ± 12%, respectively. Graft-related complications included branch kinking in 1 patient and graft rupture in 1 patient. There were no cases of graft-related thromboembolism or graft calcification. The median follow-up period was 45 [8–101, (interquartile range) 36.5, 70.9] months. The longest follow-up period until date of the currently surviving patients is 101 months.

CONCLUSIONS
Although the xenopericardium appeared to be vulnerable to fungal infection, based on the favourable results obtained, we consider that branched xenopericardial roll graft replacement using a multidisciplinary, staged approach may serve as a useful treatment option for aortic arch/arch graft infection.
Expanding the primary care workforce by integrating genetic counselors in multidisciplinary care teams
Authors: Vanneste Rachel , Bauer Sasha A , Borle Kennedy , Dreikorn Erika 
Published: October 2024

Abstract
Collectively, rare diseases are common, affecting approximately 8% of the population in Canada and the USA. Therefore, the majority of primary care (PC) clinicians will care for patients who are affected or at risk for a genetic disease. Considering the increasing ways in which genetics is being implemented into all areas of healthcare, one way to address these needs and expand the capacity of the PC workforce is through the integration of genetic counselors (GCs) into PC multidisciplinary teams. GCs are Masters-educated allied health professionals with specialized training in molecular genetics, communication, and short-term psychotherapeutic counseling. The current models of GCs in PC mimic other multidisciplinary models. Complex tasks related to genetics, such as pre- and post-test counseling, genetic test selection, and results interpretation, are conducted by GCs, which, in turn, allows physicians, nurse practitioners, and other PC providers to work at the top of their scope of practice. Quality genetics services provided by GCs improve clinical outcomes for patients and their families; the simultaneous provision of genetic education and psychological support by a GC is associated with an increase in patient knowledge, perceived personal control, decrease in distress, and can lead to positive health behavior changes, all of which are aligned with the goals of primary healthcare. With their extensive training in clinical care, medical communication, and psychotherapeutic counseling, integrating GCs into PC care teams will improve the care patients receive and allow PC clinicians to ensure their patients are at the forefront of the personalized medicine revolution.
Protecting Human Creativity in AI-Generated Music with the Introduction of an AI-Royalty Fund
Authors: Jacques Sabine , Flynn Mathew 
Published: October 2024

Abstract
Artificial Intelligence (AI) is posited to revolutionise the creative industries, prompting global calls for legislative intervention to ensure human creativity remains at the centre of the copyright system. As AI systems gain prowess in analysing and generating content, they promise new levels of creativity and innovation at accelerated pace and reduced costs compared to human production. Alongside these benefits come concerns of displacement, particularly in fields like music, where AI-generated music could potentially supplant human-authored creative endeavours. Suggestions ranging from taxation to levies have been proposed to address this challenge. This paper, however, advocates for a novel perspective: evolving copyright law to not only compensate creators for income lost to technological disruption but also to foster sustainability aligned with the principles of the Council of Europe’s European Social Charter. Proposing an ‘AI-royalty fund’ represents a more optimal approach to this dilemma. Such a fund would acknowledge the intrinsic value of music and support a sustainable and inclusive creative industry ecosystem. Essential to this vision is the role of a national collective, entrusted with administering this fund to ensure equitable distribution and uphold the interests of human authors in an AI-driven landscape, contribute to regional and local plans of growth and foster cultural diversity and innovation. In essence, as AI redefines the boundaries of creativity, adapting the copyright paradigm becomes imperative to preserving the livelihoods of human creators while promoting a resilient and sustainable creative economy.
Workplace-based learning in district health leadership and management strengthening: a framework synthesis
Authors: Kiarie Grace , Gilson Lucy , Orgill Marsha 
Published: October 2024

Abstract
Effective leadership and management has been identified as critical in enabling health systems to respond adequately to their population needs. The changing nature of low- and middle-income countries’ health systems, given resource scarcity, a high disease burden and other contextual challenges, has also led to learning—including workplace-based learning (WPBL)—being recognized as a key process supporting health system reform and transformation. This review used a framework synthesis approach in addressing the question: ‘What forms of WPBL, support leadership and management development; and how does such learning impact district health leadership and management strengthening?’. A search for English language empirical qualitative, mixed-methods and quantitative studies and grey literature published from January 1990 to May 2024 was conducted using four electronic databases (PubMed, EBSCOhost, Scopus and Web of Science). Twenty-five articles were included in the synthesis. The findings reveal that over the last decade, WPBL has received consideration as an approach for leadership and management development. While WPBL interventions differed in type and nature, as well as length of delivery, there was no conclusive evidence about which approach had a greater influence than others on strengthening district health leadership and management. However, the synthesis demonstrates the need for a focus on the sustainability and institutionalization of interventions, including the need to integrate WPBL interventions in health systems. To support sustainability and institutionalization, there should be flexibility in the design and delivery of such interventions and they are best supported through national or regional institutions.
eQTL mapping in transgenic alpha-synuclein carrying Caenorhabditis elegans recombinant inbred lines
Authors: Huang Yuqing , Wang Yiru A , van Sluijs Lisa , Vogels Demi H J , Chen Yuzhi , Tegelbeckers Vivian I P , Schoonderwoerd Steven , Riksen Joost A G , Kammenga Jan E , Harvey Simon C , Sterken Mark G 
Published: October 2024

Abstract
Protein aggregation of α-synuclein (αS) is a genetic and neuropathological hallmark of Parkinson’s disease (PD). Studies in the model nematode Caenorhabditis elegans suggested that variation of αS aggregation depends on the genetic background. However, which genes and genetic modifiers underlie individual differences in αS pathology remains unknown. To study the genotypic-phenotypic relationship of αS aggregation, we constructed a Recombinant Inbred Line (RIL) panel derived from a cross between genetically divergent strains C. elegans NL5901 and SCH4856, both harboring the human αS gene. As a first step to discover genetic modifiers 70 αS-RILs were measured for whole-genome gene expression and expression quantitative locus analysis (eQTL) were mapped. We detected multiple eQTL hot-spots, many of which were located on Chromosome V. To confirm a causal locus, we developed Introgression Lines (ILs) that contain SCH4856 introgressions on Chromosome V in an NL5901 background. We detected 74 genes with an interactive effect between αS and the genetic background, including the human p38 MAPK homologue pmk-1 that has previously been associated with PD. Together, we present a unique αS-RIL panel for defining effects of natural genetic variation on αS pathology, which contributes to finding genetic modifiers of PD.
NpCIPK6–NpSnRK1 module facilitates intersubgeneric hybridization barriers in water lily (Nymphaea) by reducing abscisic acid content
Authors: Zhou Ping , Li Jingwen , Jiang Huiyan , Yang Zhijuan , Sun Chunqing , Wang Hongyan , Su Qun , Jin Qijiang , Wang Yanjie , Xu Yingchun 
Published: October 2024

Abstract
Prefertilization hybridization barriers are the main causes of intersubgeneric hybridization challenges in water lily. However, the mechanism underlying low compatibility between pollen and stigma of water lily remains unclear. This study demonstrates that CBL-interacting protein kinase 6 (CIPK6) responded to the signaling exchange between incompatible pollen and stigma through interactions with SNF1-related kinase 1 (SnRK1) and promotes the accumulation of SnRK1 protein. Activated SnRK1 interacted with 9-cis-epoxycarotenoid dioxygenase 2 (NCED2) to promote its degradation, thereby inhibiting abscisic acid (ABA) synthesis. A decrease in ABA content in the stigma impaired the ABA-mediated removal of reactive oxygen species (ROS), ultimately resulting in the rejection of the incompatible pollen by the stigma. Our results highlight the essential role of the NpCIPK6–NpSnRK1–NpNCED2 module in conferring intersubgeneric hybridization barriers in water lily by interfering with ABA synthesis and promoting ROS accumulation. This study offers valuable mechanistic insights into cellular signaling and reproductive barriers in water lily as well as across other biological contexts.
Microbial and Transcriptomic Landscape Associated With Neutrophil Extracellular Traps in Perianal Fistulizing Crohn’s Disease
Authors: Cao Dongxing , Hu Muni , Yang Nailin , Qian Keyu , Hong Jie , Tang Jian , Bian Yuhai , Zhang Cheng , Wang Xiaohui , Wu Guangyu , Chen Haoyan , Zhang Ye , Wang Zheng , Cui Zhe 
Published: October 2024

Abstract

Background
Perianal fistulizing Crohn’s disease (pfCD) poses significant healing challenges, closely associated with neutrophil extracellular traps (NETs). This study aimed to investigate the microbe–host interactions influencing NETs in pfCD.

Methods
From January 2019 to July 2022, patients with pfCD were screened at Ren Ji Hospital. Patients in remission following comprehensive treatment were recruited. We documented clinical characteristics, medication regimens, healing outcomes, and infliximab levels in fistula tissues. NET positivity was confirmed by positive results in citrullinated histone H3 (CitH3) enzyme-linked immunosorbent assay (ELISA) and dual immunofluorescence staining for myeloperoxidase and CitH3. Microbial and transcriptomic profiles from fistula tissues, obtained during surgery, were analyzed using 16S rRNA gene sequencing and RNA sequencing. Differences in microbiome and transcriptomic profiles were evaluated, and their relationships were assessed using Mantel’s and Spearman’s coefficients.

Results
Significant differences in microbial communities were found between groups (P = .007). Representatively differential microbes such as Prevotella bivia, Streptococcus gordonii, and Bacteroides dorei were enriched in NETs-positive fistulas (P &lt; .05). Functional analysis of microbes revealed reduced ubiquinol biosynthesis and butanoate production in NETs-negative fistulas (P &lt; .05). Transcriptomic analysis indicated increased neutrophil and monocyte infiltration in NETs-positive fistulas, associated with pathways involving bacterial response, neutrophil chemotaxis, secretory processes, and peptidase activity (P &lt; .05). Species prevalent in NETs-positive fistulas correlated positively with immune responses and wound healing pathways, whereas bacteria in NETs-negative fistulas correlated negatively. NETs were negatively associated with tissue infliximab levels (P = .001) and healing outcomes (P = .025).

Conclusions
Our findings reveal unique microbial and transcriptomic signatures associated with NETs in pfCD, highlighting their profound influence on clinical outcomes.
Twisted Mahler Discrete Residues
Authors: Arreche Carlos E , Zhang Yi 
Published: October 2024

Abstract
Recently we constructed Mahler discrete residues for rational functions and showed they comprise a complete obstruction to the Mahler summability problem of deciding whether a given rational function $f(x)$ is of the form $g(x^{p})-g(x)$ for some rational function $g(x)$ and an integer $p&gt; 1$. Here we develop a notion of $\lambda $-twisted Mahler discrete residues for $\lambda \in \mathbb{Z}$, and show that they similarly comprise a complete obstruction to the twisted Mahler summability problem of deciding whether a given rational function $f(x)$ is of the form $p^{\lambda } g(x^{p})-g(x)$ for some rational function $g(x)$ and an integer $p&gt;1$. We provide some initial applications of twisted Mahler discrete residues to differential creative telescoping problems for Mahler functions and to the differential Galois theory of linear Mahler equations.
Review: Known, Emerging, and Remerging Pharyngitis Pathogens
Authors: Caldwell Jane M , Ledeboer Nathan A , Boyanton Bobby L 
Published: October 2024

Abstract
Pharyngitis is an inflammatory condition of the pharynx and/or tonsils commonly seen in both children and adults. Viruses and bacteria represent the most common encountered etiologic agents—yeast/fungi and parasites are infrequently implicated. Some of these are predominantly observed in unique populations (eg, immunocompromised or unvaccinated individuals). This article (part 1 of 3) summarizes the impact of acute pharyngitis on the health care system and reviews the etiologic agents of acute pharyngitis, including both emerging and reemerging pathogens that health care providers should consider when evaluating their patients. Finally, it sets the stage for parts 2 and 3, which discuss the current and evolving state of diagnostic testing for acute pharyngitis.
Repeated horizontal acquisition of lagriamide-producing symbionts in Lagriinae beetles
Authors: Uppal Siddharth , Waterworth Samantha C , Nick Alina , Vogel Heiko , Flórez Laura V , Kaltenpoth Martin , Kwan Jason C 
Published: October 2024

Abstract
Microbial symbionts associate with multicellular organisms on a continuum from facultative associations to mutual codependency. In the oldest intracellular symbioses there is exclusive vertical symbiont transmission, and co-diversification of symbiotic partners over millions of years. Such symbionts often undergo genome reduction due to low effective population sizes, frequent population bottlenecks, and reduced purifying selection. Here, we describe multiple independent acquisition events of closely related defensive symbionts followed by genome erosion in a group of Lagriinae beetles. Previous work in Lagria villosa revealed the dominant genome-eroded symbiont of the genus Burkholderia produces the antifungal compound lagriamide, protecting the beetle’s eggs and larvae from antagonistic fungi. Here, we use metagenomics to assemble 11 additional genomes of lagriamide-producing symbionts from 7 different host species within Lagriinae from 5 countries, to unravel the evolutionary history of this symbiotic relationship. In each host, we detected one dominant genome-eroded Burkholderia symbiont encoding the lagriamide biosynthetic gene cluster. However, we did not find evidence for host–symbiont co-diversification or for monophyly of the lagriamide-producing symbionts. Instead, our analyses support a single ancestral acquisition of the gene cluster followed by at least four independent symbiont acquisitions and subsequent genome erosion in each lineage. By contrast, a clade of plant-associated relatives retained large genomes but secondarily lost the lagriamide gene cluster. Our results, therefore, reveal a dynamic evolutionary history with multiple independent symbiont acquisitions characterized by a high degree of specificity and highlight the importance of the specialized metabolite lagriamide for the establishment and maintenance of this defensive symbiosis.
The Autocracy Bias: Evaluating Democratic Citizens’ Perception of Human Rights Violations in Policy Proposals Abroad
Authors: Barceló Joan , Sheen Greg Chih-Hsin 
Published: October 2024

Abstract
Do citizens in democracies view foreign policies as more violative of human rights based on the governing regime’s type? We hypothesize that in democratic societies, there is an inherent association of autocracy with human rights violations, leading citizens to believe that policies from authoritarian governments infringe upon human rights more than those from democracies, even when the policies are seemingly identical. We evaluate this autocracy bias by gathering evidence from nine survey experiments across seven democracies. Consistently, we find that citizens of democratic countries are more likely to consider a policy to be a violation of human rights if China adopts it, compared to when a seemingly identical policy is adopted by Spain or Italy. We find that this perception extends to eleven other authoritarian regimes, and a mechanism experiment reveals that regime type is the driving force behind this differential evaluation. This study contributes to the understanding of how citizens in democratic societies perceive respect for human rights in foreign countries.
Development and characterization of nanoemulsions containing Lippia origanoides Kunth essential oil and their antifungal potential against Candida albicans
Authors: Prado Júlio César Sousa , de Aguiar Francisca Lidiane Linhares , Prado Guilherme Mendes , Nascimento Joice Farias do , de Sousa Natália Vasconcelos , Barbosa Francisco Cesar Barroso , Lima Danielle Malta , Rodrigues Tigressa Helena Soares , Bessa Nathália Uchôa de Castro , Abreu Flavia Oliveira Monteiro da Silva , Fontenelle Raquel Oliveira dos Santos 
Published: October 2024

Abstract

Aims
Nanoemulsions based on plant essential oils have shown promise as alternatives against fungal pathogens by increasing the solubility and bioavailability of the active compounds of essential oils, which can improve their efficacy and safety. In the present study, we aimed to prepare and characterize nanoemulsions of Lippia origanoides essential oil, and analyze their antifungal activity against C. albicans in planktonic and biofilm form. Additionally, we sought to verify their cytotoxicity.

Methods and results
Alginate nanoemulsions were prepared with different concentrations of essential oil, sunflower oil, and surfactant to investigate ideal formulations regarding stability and antifungal efficiency. The results showed the nanoemulsions remained stable for longer than 60 days, with acidic pH, particle sizes ranging from 180.17 ± 6.86 nm to 497.85 ± 253.50 nm, zeta potential from −60.47 ± 2.25 to −43.63 ± 12, and polydispersity index from 0.004 to 0.622. The photomicrographs revealed that the addition of sunflower oil influenced the formation of the particles, forming nanoemulsions. The antifungal results of the essential oil and nanoemulsions showed that the MIC ranged from 0.078 to 0.312 mg ml−1. The nanoemulsions were more effective than the free essential oil in eradicating the biofilm, eliminating up to 89.7% of its mass. With regard to cytotoxicity, differences were found between the tests with VERO cells and red blood cells, and the nanoemulsions were less toxic to red blood cells than the free essential oil.

Conclusions
These results show that nanoemulsions have antifungal potential against strains of C. albicans in planktonic and biofilm forms.
Airborne metofluthrin, a pyrethroid repellent, does not impact foraging honey bees
Authors: Couvillon Margaret J , Hainze John , Bizon Connor , Johnson Lindsay E , McKellips Ian F , McMillan Benjamin E , Ohlinger Bradley D , Ostrom Robert B J , Schürch Roger 
Published: October 2024

Abstract
Outdoor spatial mosquito repellents, such as mosquito coils or heating devices, release pyrethroid insecticides into the air to provide protection from mosquitoes within a defined area. This broadcast discharge of pyrethroids into the environment raises concern about the effect on non-target organisms. A previous study found that prallethrin discharged from a heating device did not affect honey bee (Apis mellifera L.) [Hymenoptera: Apidae] foraging or recruitment. In this second study, there was no significant difference in foraging frequency (our primary outcome), waggle dance propensity, or persistency in honey bees collecting sucrose solution between those exposed to metofluthrin from a different heating device and bees exposed to a non-metofluthrin control. One measure, waggle dance frequency, was higher in the metofluthrin treatment than the control but this outcome was likely a spurious result due to the small sample size. The small particle size of the emissions, averaging 4.43 µm, from the heated spatial repellent products, which remain airborne with little settling, may play an important role in the lack of effect found on honey bee foraging.
A new species of Tube-nosed Bat (Chiroptera: Vespertilionidae: Murina) from Qinghai–Tibet Plateau, China
Authors: Wang Xiaoyun , Han Xuesong , Csorba Gábor , Wu Yi , Chen Huaiqing , Zhao Xiang , Dong Zhengyi , Yu Wenhua , Lu Zhi 
Published: October 2024

Abstract
In 2018, an adult male of a small-sized Tube-nosed Bat (Chiroptera: Vespertilionidae: Murina) was captured at an arid cave located on the Qinghai–Tibet Plateau in Yushu City, Qinghai Province, China. Despite external morphological similarities with those of M. harpioloides and M. chrysochaetes, the individual in question displays explicit craniodental differences that distinguish it from either species. Morphological and morphometric evidence, coupled with phylogenetic analyses utilizing the mitochondrial COI gene, confirmed that it represents a distinct and still unknown species of Murina, described herewith as M. yushuensis sp. nov. Our research highlights the importance of future surveys aimed at exploring cryptic species diversity in the Qinghai–Tibet Plateau and adjacent under-surveyed regions.