bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neonatal exposure to high oxygen levels leads to impaired ischemia-induced neovascularization in adulthood
Authors: Mathieu Raphael , Dussault Sylvie , Desjarlais Michel , Rivard François , Dhahri Wahiba , Cloutier Anik , Nuyt Anne-Monique , Rivard Alain 
Published: October 2017

AbstractAdverse perinatal conditions can lead to developmental programming of cardiovascular diseases. Prematurely born infants are often exposed to high oxygen levels, which in animal models has been associated with endothelial dysfunction, hypertension, and cardiac remodeling during adulthood. Here we found that adult mice that have been transiently exposed to O2 after birth show defective neovasculariation after hindlimb ischemia, as demonstrated by impaired blood flow recovery, reduced vascular density in ischemic muscles and increased tissue damages. Ischemic muscles isolated from mice exposed to O2 after birth exhibit increased oxidative stress levels and reduced expression of superoxide dismutase 1 (SOD1) and vascular endothelial growth factor (VEGF). Pro-angiogenic cells (PACs) have been shown to have an important role for postnatal neovascularisation. We found that neonatal exposure to O2 is associated with reduced number of PACs in adults. Moreover, the angiogenic activities of both PACs and mature mouse aortic endothelial cells (MAECs) are significantly impaired in mice exposed to hyperoxia after birth. Our results indicate that neonatal exposure to high oxygen levels leads to impaired ischemia-induced neovascularization during adulthood. The mechanism involves deleterious effects on oxidative stress levels and angiogenic signals in ischemic muscles, together with dysfunctional activities of PACs and mature endothelial cells.
Involvement of the Vitamin D Receptor in Energy Metabolism Revealed by Profiling of Lysine Succinylome of White Adipose Tissue
Authors: Su Han , Lou Yan , Fu Yu , Zhang Yalin , Liu Ning , Liu Zuwang , Zhou Yanyan , Kong Juan 
Published: October 2017

AbstractLysine succinylation, emerging as a novel post-translational modification, is closely related to the regulation of diverse biological processes, including many aspects of metabolism. Growing evidence suggests that low vitamin D status might exert an adverse impact on energy balance, adipogenesis and inflammation in white adipose tissue (WAT). However, whether there are any interactions between vitamin D and lysine succinylation still remains unknown. Here, combining high-affinity enrichment of lysine succinylated peptides with mass spectrometry and bioinformatics analysis, we reported the systematic profiling of the lysine succinylome, identifying 209 sites occurring on 159 proteins were up-regulated, 3 sites in 3 proteins were down-regulated in vitamin D receptor (VDR)−/− mice. Bioinformatics analysis reveals potential impacts of lysine succinylation on diverse biological processes and molecular functions, especially on carbon biotransformation, fatty acid metabolism and TCA cycle. Furthermore, eight unique motifs surrounding the succinylation sites were validated. Collectively, our findings demonstrate the first comprehensive profiling of WAT succinylome in VDR−/− mice, and provide crucial clues for further elucidating the underlying mechanisms of the involvement of the VDR in energy metabolism.
Effects of Supra-Physiological Levothyroxine Dosages on Liver Parameters, Lipids and Lipoproteins in Healthy Volunteers: A Randomized Controlled Crossover Study
Authors: Sjouke Barbara , Elbers Laura P. B. , van Zaane Bregje , Kastelein John J. P. , Hovingh G. Kees , Gerdes Victor E. A. 
Published: October 2017

AbstractEprotirome, a liver specific thyroid hormone agonist, was shown to induce significant increases in markers of liver injury along with a modest decrease in atherogenic lipids and lipoproteins. To get more insight into whether these effects on liver parameters were compound specific or the effect of mimicking thyrotoxicosis, we studied the effects of supra-physiological levothyroxine dosages on liver parameters, lipids and lipoproteins. We used data of a single-blinded, randomized controlled crossover trial. Herein, healthy volunteers received levothyroxine or no medication for 14 days. Thyroid hormone excess did not induce clinically relevant changes in liver parameters, while significant reductions in total cholesterol, low-density lipoprotein-cholesterol as well as apolipoprotein-B levels were observed in the intervention periods compared with the control periods. Supra-physiological thyroid hormone levels did not induce clinically relevant increases in markers of liver injury after 2 weeks of exposure, while it reduced total cholesterol, low-density lipoprotein cholesterol and apolipoprotein B levels. This suggests that the effects of eprotirome on liver parameters in previous studies were either off-target and compound specific or due to drug-drug interaction at the level of the liver. The results of our study are relevant for the development of novel thyroid hormone agonists to reduce atherogenic lipoproteins.
Self-cleaning liner for halogenated hydrocarbon control in landfill leachate
Authors: He Shichong , Zhu Lizhong 
Published: October 2017

AbstractSorptive landfill liners can prevent the migration of the leachate pollutants. However, their sorption ability will decrease over time. A method should be developed to maintain the sorption ability of landfill liners. In this study, we combined cetyltrimethylammonium bromide-bentonite (CTMAB-bentonite) and zero-valent iron (ZVI) to develop a self-cleaning liner that can retain its sorption ability for a long period. Batch experiments and calculation simulations were employed to analyse the sorption ability of this liner material and the ecological risk of halogenated hydrocarbons. The results showed that CTMAB-bentonite could sorb halogenated hydrocarbons well, with saturated sorption capacities (Qm) of 10.2, 14.5, 6.69, 18.5, 29.4, and 49.7 mg·g-1 for dichloroethane (DCA), trichloroethane (TCA), dichloroethene (DCE), trichloroethylene (TCE), tetrachloroethylene (PCE), and 1,3- dichloropropene (1,3-DCP), respectively. Using the mixture of 0.5 g iron and 0.5 g CTMAB-bentonite could dramatically increase the removal efficiency of DCE, TCE, and PCE. The reaction with ZVI did not change the structure of CTMAB-bentonite and its sorption ability remained consistent. Calculation results suggested that the self-cleaning landfill liner would dramatically decrease the hazard index (HI) of the eluate. However, the humic acid and salt in leachate would cause a reduction in the removal of halogenated hydrocarbons.
Lensless Tomographic Imaging of Near Surface Structures of Frozen Hydrated Malaria-Infected Human Erythrocytes by Coherent X-Ray Diffraction Microscopy
Authors: Frank Viktoria , Chushkin Yuriy , Fröhlich Benjamin , Abuillan Wasim , Rieger Harden , Becker Alexandra S. , Yamamoto Akihisa , Rossetti Fernanda F. , Kaufmann Stefan , Lanzer Michael , Zontone Federico , Tanaka Motomu 
Published: October 2017

AbstractLensless, coherent X-ray diffraction microscopy has been drawing considerable attentions for tomographic imaging of whole human cells. In this study, we performed cryogenic coherent X-ray diffraction imaging of human erythrocytes with and without malaria infection. To shed light on structural features near the surface, “ghost cells” were prepared by the removal of cytoplasm. From two-dimensional images, we found that the surface of erythrocytes after 32 h of infection became much rougher compared to that of healthy, uninfected erythrocytes. The Gaussian roughness of an infected erythrocyte surface (69 nm) is about two times larger than that of an uninfected one (31 nm), reflecting the formation of protein knobs on infected erythrocyte surfaces. Three-dimensional tomography further enables to obtain images of the whole cells with no remarkable radiation damage, whose accuracy was estimated using phase retrieval transfer functions to be as good as 64 nm for uninfected and 80 nm for infected erythrocytes, respectively. Future improvements in phase retrieval algorithm, increase in degree of coherence, and higher flux in combination with complementary X-ray fluorescence are necessary to gain both structural and chemical details of mesoscopic architectures, such as cytoskeletons, membraneous structures, and protein complexes, in frozen hydrated human cells, especially under diseased states.
A single episode of high intensity sound inhibits long-term potentiation in the hippocampus of rats
Authors: de Deus J. L. , Cunha A. O. S. , Terzian A. L. , Resstel L. B. , Elias L. L. K. , Antunes-Rodrigues J. , Almeida S. S. , Leão R. M. 
Published: October 2017

AbstractExposure to loud sounds has become increasingly common. The most common consequences of loud sound exposure are deafness and tinnitus, but emotional and cognitive problems are also associated with loud sound exposure. Loud sounds can activate the hipothalamic-pituitary-adrenal axis resulting in the secretion of corticosterone, which affects hippocampal synaptic plasticity. Previously we have shown that long-term exposure to short episodes of high intensity sound inhibited hippocampal long-term potentiation (LTP) without affecting spatial learning and memory. Here we aimed to study the impact of short term loud sound exposure on hippocampal synaptic plasticity and function. We found that a single minute of 110 dB sound inhibits hippocampal Schaffer-CA1 LTP for 24 hours. This effect did not occur with an 80-dB sound exposure, was not correlated with corticosterone secretion and was also observed in the perforant-dentate gyrus synapse. We found that despite the deficit in the LTP these animals presented normal spatial learning and memory and fear conditioning. We conclude that a single episode of high-intensity sound impairs hippocampal LTP, without impairing memory and learning. Our results show that the hippocampus is very responsive to loud sounds which can have a potential, but not yet identified, impact on its function.
A data-driven statistical model that estimates measurement uncertainty improves interpretation of ADC reproducibility: a multi-site study of liver metastases
Authors: Pathak Ryan , Ragheb Hossein , Thacker Neil A. , Morris David M. , Amiri Houshang , Kuijer Joost , deSouza Nandita M. , Heerschap Arend , Jackson Alan 
Published: October 2017

AbstractApparent Diffusion Coefficient (ADC) is a potential quantitative imaging biomarker for tumour cell density and is widely used to detect early treatment changes in cancer therapy. We propose a strategy to improve confidence in the interpretation of measured changes in ADC using a data-driven model that describes sources of measurement error. Observed ADC is then standardised against this estimation of uncertainty for any given measurement. 20 patients were recruited prospectively and equitably across 4 sites, and scanned twice (test-retest) within 7 days. Repeatability measurements of defined regions (ROIs) of tumour and normal tissue were quantified as percentage change in mean ADC (test vs. re-test) and then standardised against an estimation of uncertainty. Multi-site reproducibility, (quantified as width of the 95% confidence bound between the lower confidence interval and higher confidence interval for all repeatability measurements), was compared before and after standardisation to the model. The 95% confidence interval width used to determine a statistically significant change reduced from 21.1 to 2.7% after standardisation. Small tumour volumes and respiratory motion were found to be important contributors to poor reproducibility. A look up chart has been provided for investigators who would like to estimate uncertainty from statistical error on individual ADC measurements.
Orbital-dependent charge dynamics in MnP revealed by optical study
Authors: Zheng P. , Xu Y. J. , Wu W. , Xu G. , Lv J. L. , Lin F. K. , Wang P. , Yang Yi-feng , Luo J. L. 
Published: October 2017

AbstractUnconventional superconductivity often emerges at the border of long-range magnetic orders. Understanding the low-energy charge dynamics may provide crucial information on the formation of superconductivity. Here we report the unpolarized/polarized optical conductivity study of high quality MnP single crystals at ambient pressure. Our data reveal two types of charge carriers with very different lifetimes. In combination with the first-principles calculations, we show that the short-lifetime carriers have flat Fermi sheets which become gapped in the helimagnetic phase, causing a dramatic change in the low-frequency optical spectra, while the long-lifetime carriers are anisotropic three-dimensional like which are little affected by the magnetic transitions and provide major contributions to the transport properties. This orbital-dependent charge dynamics originates from the special crystal structure of MnP and may have an influence on the unconventional superconductivity and its interplay with helimagnetism at high pressures.
Pearls in running drops on an inclined glass substrate excited by Lamb waves
Authors: Liang Wei , Tietze Sabrina 
Published: October 2017

AbstractWe demonstrate that pearling droplets will be released from droplets as they sliding down a partially wetting glass plate excited by Lamb waves. During the movement, we find that the transitions at generating pearling are independent of the drop size and depend only on a critical capillary number Ca. Further up, the position of the pearls must be at or around the droplet’s advancing or receding end of the initial state.
Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system
Authors: Aoyama Katsuyuki , Kuroda Shinji , Morihiro Toshiaki , Kanaya Nobuhiko , Kubota Tetsushi , Kakiuchi Yoshihiko , Kikuchi Satoru , Nishizaki Masahiko , Kagawa Shunsuke , Tazawa Hiroshi , Fujiwara Toshiyoshi 
Published: October 2017

AbstractOncolytic virotherapy has the disadvantage of being unsuitable for systemic delivery due to immune elimination. Liposomal encapsulation is well-recognized to reduce immune elimination and enhance the stability of drugs in the bloodstream. In the present study, the potential of liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus (TelomeScan) expressing GFP (Lipo-pTS) as an oncolytic adenoviral agent suitable for systemic delivery was investigated. Lipo-pTS, which has a diameter of 40–50 nm, showed potent antitumor effects on HCT116 colon carcinoma cells in vitro and in vivo. Tumor selectivity of Lipo-pTS was independent of coxsackie and adenovirus receptor (CAR). Importantly, Lipo-pTS reduced production of adenovirus-neutralizing antibodies (AdNAbs) after intravenous administration into immune-competent mice compared to TelomeScan, and even in the presence of AdNAbs, Lipo-pTS maintained strong cytotoxicity. In conclusion, Lipo-pTS has the potential to become an oncolytic adenoviral agent suitable for systemic delivery with the characteristics of CAR-independent antitumor activity and a stealth effect on the immune system.
Disrupting environmental crime at the local level: an operational perspective
Authors: Barrett Stoyan , White Rob 
Published: October 2017

AbstractThe multi-dimensional nature of environmental crimes requires innovative means to combat them. This paper examines the nature and dynamics of one particular collaborative law enforcement approach directed at environmental crime. While much interest in multi-agency and multi-pronged approaches has been generated in recent years, especially as this pertains to environmental crime at the international and regional levels, this paper provides a detailed description of how this can occur at the local level. Based on a case study of nefarious activities relating to illegal waste management in Durham and surrounding areas (located in the North East of England), the paper emphasises the importance of ‘disruption’ as an important operational concept, and how inter-agency cooperation under skilled leadership and with clear purpose can lead to tangible enforcement outcomes. As the article demonstrates, particular agencies on their own have limited impact and do not always address the issue of how to take away an organised criminal group’s ability to function. By contrast, multi-agency work enables authorities to work in a united front and thus to succeed in disrupting criminal activities causing environmental harm.
From manipulation to social interaction
Authors: Ponsford Dan 
Published: October 2017

AbstractThis paper is about constructional change that is brought about through change in non-linguistic practice. The English construction of interest is one that speakers use to initiate bets with their addressees. Its verb islay, its subject is the speaker, and its direct object is the stake the speaker proposes to risk. It is argued that the motivation for the use oflaycomes partly from the practice of laying down stakes when making bets. However, it is shown that over the seventeenth to the nineteenth centuries this practice declines, weakening the basis for a physical interpretation and leading hearers to attend instead to the speaker-addressee relation. Concurrently, this relation is increasingly expressed through the use of a dative argument. This development is discussed in relation toAriel et al.’s (2015)account of added datives.
Head-Driven Phrase Structure Grammar, Sign-Based Construction Grammar, and Fluid Construction Grammar
Authors: Müller Stefan 
Published: October 2017

AbstractVan Trijp (2013,2014) claims that Sign-Based Construction Grammar (SBCG) and Head-Driven Phrase Structure Grammar (HPSG) are fundamentally different from Fluid Construction Grammar (FCG). He claims that the former approaches are generative ones while the latter is a cognitive-functional one. I argue that it is not legitimate to draw these distinctions on the basis of what is done in FCG. Van Trijp claims that there are differences in the scientific model, the linguistic approach, formalization, the way constructions are seen, and in terms of processing. This paper discusses all these alleged differences. Van Trijp also claims that his cognitive-functional approach is superior in terms of completeness, explanatory adequacy, and theoretical parsimony. In order to facilitate a discussion and comparison, I introduce the reader to basic assumptions made in FCG and the analyses suggested by Van Trijp: I first deal with the representations that are used in FCG, talk about argument structure constructions, the combination operations fusion and merging that are used in FCG, I than discuss the analysis of nonlocal dependencies and show that the suggested FCG analysis is not explanatorily adequate since it is not descriptively adequate and that a full formalization of approaches with discontinuous constituents is not more parsimonious than existing HPSG analyses either. After the discussion of specific analyses, I then provide a detailed comparison of FCG and SBCG/HPSG and discuss questions like the competence/performance distinction, mathematical formalization vs. computer implementation, fuzziness and fluidity in grammars, and permissiveness of theories. I conclude that HPSG, SBCG, and FCG belong to the same family of theories and that all claims to the contrary are unjustified.
LncRNA PVT1 predicts prognosis and regulates tumor growth in prostate cancer
Authors: Yang Jin , Li Cuirong , Mudd Ashley , Gu Xiao 
Published: October 2017

Abstract
Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1(PVT1) was aberrantly expressed in various cancers and is associated with tumor prognosis. Here, we aim to investigate its function in prostate cancer. Small interfering RNA against PVT1 was transfected into prostate cancer cell lines and cell growth and apoptosis were analyzed. Our results showed that PVT1 was overexpressed in prostate cancer tissues and cells. Higher levels of PVT1 indicated poorer overall survival and disease-free survival. A significant association was found between PVT1 expression and tumor stage. Besides, PVT1 knockdown significantly inhibited prostate cancer growth in vivo and in vitro and promoted cell apoptosis. PVT1 knockdown also significantly upregulated the expression of cleaved caspase-3 and cleaved caspase-9, but downregulated the expression of c-Myc in prostate cancer cell lines. Our results suggest that PVT1 played an oncogenic role in prostate cancer and could be used as a potential biomarker for diagnosis of prostate cancer.
New triterpene saponins from the roots of Acacia macrostachya (Mimosaceae)
Authors: Tchoukoua Abdou , Tabopda Turibio Kuiate , Usukhbayar Narandulam , Kimura Ken-ichi , Kwon Eunsang , Momma Hiroyuki , Koseki Takuya , Shiono Yoshihito , Ngadjui Bonaventure Tchaleu 
Published: October 2017

Abstract
Four new oleanane-type saponins, macrostachyaosides A, B, C, and D (1–4) were isolated from the roots of Acacia macrostachya. Their structures were elucidated on the basis of extensive 1D- and 2D-NMR data and HR-ESI-MS analyses. At concentrations of 100 μM of each compounds, none of the tested compounds caused a significant growth reduction against HL60 cells.
Chromatin accessibility prediction via a hybrid deep convolutional neural network
Authors: Liu Qiao , Xia Fei , Yin Qijin , Jiang Rui 
Published: October 2017

AbstractMotivationA majority of known genetic variants associated with human-inherited diseases lie in non-coding regions that lack adequate interpretation, making it indispensable to systematically discover functional sites at the whole genome level and precisely decipher their implications in a comprehensive manner. Although computational approaches have been complementing high-throughput biological experiments towards the annotation of the human genome, it still remains a big challenge to accurately annotate regulatory elements in the context of a specific cell type via automatic learning of the DNA sequence code from large-scale sequencing data. Indeed, the development of an accurate and interpretable model to learn the DNA sequence signature and further enable the identification of causative genetic variants has become essential in both genomic and genetic studies.ResultsWe proposed Deopen, a hybrid framework mainly based on a deep convolutional neural network, to automatically learn the regulatory code of DNA sequences and predict chromatin accessibility. In a series of comparison with existing methods, we show the superior performance of our model in not only the classification of accessible regions against background sequences sampled at random, but also the regression of DNase-seq signals. Besides, we further visualize the convolutional kernels and show the match of identified sequence signatures and known motifs. We finally demonstrate the sensitivity of our model in finding causative noncoding variants in the analysis of a breast cancer dataset. We expect to see wide applications of Deopen with either public or in-house chromatin accessibility data in the annotation of the human genome and the identification of non-coding variants associated with diseases.Availability and implementationDeopen is freely available at https://github.com/kimmo1019/Deopen.Supplementary informationSupplementary data are available at Bioinformatics online.
Packing Curves on Surfaces with Few Intersections
Authors: Aougab Tarik , Biringer Ian , Gaster Jonah 
Published: October 2017

Abstract
Przytycki has shown that the size $\mathcal{N}_{k}(S)$ of a maximal collection of simple closed curves that pairwise intersect at most $k$ times on a topological surface $S$ grows at most as $|\chi(S)|^{k^{2}+k+1}$. In this article, we narrow Przytycki’s bounds, obtaining \[ \mathcal{N}_{k}(S) =O \left( \frac{ |\chi|^{3k}}{ ( \log |\chi| )^2 } \right)\!. \] In particular, the size of a maximal 1-system grows sub-cubically in $|\chi(S)|$. The proof uses a circle packing argument of Aougab and Souto and a bound for the number of curves of length at most $L$ on a hyperbolic surface. When the genus $g$ is fixed and the number of punctures $n$ grows, we use a different argument to show \[ \mathcal{N}_{k}(S) \leq O(n^{2k+2}). \] This may be improved when $k=2$, and we obtain the sharp estimate $\mathcal{N}_2(S)=\Theta(n^3)$.