bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry
Authors: Elmongy Hatem , Ahmed Hytham , Wahbi Abdel‐Aziz , Amini Ahmad , Colmsjö Anders , Abdel‐Rehim Mohamed 
Published: January 2016

AbstractA sensitive, accurate and reliable bioanalytical method for the enantioselective determination of metoprolol in plasma and saliva samples utilizing liquid chromatography–electrospray ionization tandem mass spectrometry was developed and validated. Human plasma and saliva samples were pretreated by microextraction by packed sorbent (MEPS) prior to analysis. A new MEPS syringe form with two inputs was used. Metoprolol enantiomers and internal standard pentycaine (IS) were eluted from MEPS sorbent using isopropanol after removal of matrix interferences using aliquots of 5% methanol in water. Complete separation of metoprolol enantiomers was achieved on a Cellulose‐SB column (150 × 4.6 mm, 5 μm) using isocratic elution with mobile phase 0.1% ammonium hydroxide in hexane–isopropanol (80:20, v/v) with a flow rate of 0.8 mL/min. A post‐column solvent‐assisted ionization was applied to enhance metoprolol ionization signal in positive mode monitoring (+ES) using 0.5% formic acid in isopropanol at a flow rate of 0.2 mL/min. The total chromatographic run time was 10 min for each injection. The detection of metoprolol in plasma and saliva samples was performed using triple quadrupole tandem mass spectrometer in +ES under the following mass transitions: m/z 268.08 → 72.09 for metoprolol and m/z 303.3 → 154.3 for IS. The linearity range was 2.5–500 ng/mL for both R‐ and S‐metoprolol in plasma and saliva. The limits of detection and quantitation for both enantiomers were 0.5 and 2.5 ng/mL respectively, in both matrices (plasma and saliva). The intra‐ and inter‐day precisions were presented in terms of RSD values for replicate analysis of quality control samples and were <5%; the accuracy of determinations varied from 96 to 99%. The method was able to determine the therapeutic levels of metoprolol enantiomers in both human plasma and saliva samples successfully, which can aid in therapeutic drug monitoring in clinical laboratories. Copyright © 2016 John Wiley & Sons, Ltd.
Connecting Active‐Site Loop Conformations and Catalysis in Triosephosphate Isomerase: Insights from a Rare Variation at Residue 96 in the Plasmodial Enzyme
Authors: Pareek Vidhi , Samanta Moumita , Joshi Niranjan V , Balaram Hemalatha , Murthy Mathur R. N. , Balaram Padmanabhan 
Published: January 2016

AbstractDespite extensive research into triosephosphate isomerases (TIMs), there exists a gap in understanding of the remarkable conjunction between catalytic loop‐6 (residues 166–176) movement and the conformational flip of Glu165 (catalytic base) upon substrate binding that primes the active site for efficient catalysis. The overwhelming occurrence of serine at position 96 (98 % of the 6277 unique TIM sequences), spatially proximal to E165 and the loop‐6 residues, raises questions about its role in catalysis. Notably, Plasmodium falciparum TIM has an extremely rare residue—phenylalanine—at this position whereas, curiously, the mutant F96S was catalytically defective. We have obtained insights into the influence of residue 96 on the loop‐6 conformational flip and E165 positioning by combining kinetic and structural studies on the PfTIM F96 mutants F96Y, F96A, F96S/S73A, and F96S/L167V with sequence conservation analysis and comparative analysis of the available apo and holo structures of the enzyme from diverse organisms.
Molecular Recognition of Parallel DNA Quadruplex d(TTAGGGT)4 by Mitoxantrone: Binding with 1:2 Stoichiometry Leading to Thermal Stabilization and Telomerase Inhibition
Authors: Tripathi Sweta , Pradeep Tarikere Palakshan , Barthwal Ritu 
Published: January 2016

AbstractThe interaction of the anthraquinone derivative mitoxantrone, a semisynthetic anti‐cancer drug with two non‐planar side chains, with heptamer G‐quadruplex d(TTAGGGT)4, which contains the human telomere DNA sequence, was evaluated by differential scanning calorimetry, fluorescence Job plotting, absorption, and NMR and CD spectroscopy. Binding led to thermal stabilization of DNA (ΔTm=13–20 °C). The spectra revealed that two mitoxantrone molecules bind externally at two sites of the DNA quadruplex as monomers, by partial insertion of the chromophore and side‐chain interaction at the grooves. The inhibition of telomerase (IC50=2 μm), as determined by a TRAP assay, can be attributed to thermal stabilization of the DNA quadruplex because of the interactions with mitoxantrone. The studies revealed highly specific molecular recognition between a ligand and a parallel‐stranded G‐quadruplex; this might serve as a platform for the rational design of new drugs.
Prolactin receptor and osteogenic induction of prolactin in human periodontal ligament fibroblasts
Authors: Surarit Rudee , Krishnamra Nateetip , Seriwatanachai Dutmanee 
Published: January 2016

AbstractProlactin is an important hormone involved in the interaction between maternal, extraembryonic, and fetal tissues that remains in high levels during the entire duration of pregnancy. Although many systemic alterations occur during pregnancy, such as hormonal changes, that are known to be associated with periodontitis and tooth loss, PRL function in human periodontal ligament fibroblasts (HPDLF) had never been studied. Herein, we investigated the role of PRL in the regulation of HPDLF proliferation and differentiation. HPDLF were cultured in differentiating medium with various concentrations of PRL. The present study demonstrated that HPDLF and primary human PDL cells that were extracted for orthodontic purpose expressed both short and long isoforms of PRLR mRNA and its proteins. An incubation with of high concentration of PRL (600 and 1,000 ng/mL) modestly decreased the HPDLF number. In contrast, PRL at a non‐reproductive level (10 ng/mL) and pregnant level (100 ng/mL) significantly upregulated the markers of osteogenesis, such as RUNX2, BMP2, and POSTN, but not SOX9. Mineral nodule formation was induced, whereas proteoglycan accumulation was reduced by PRL suggesting that HPDLF were undergoing differentiation into preosteoblastic cells. In conclusion, the presence of hPRLR in human PDL together with PRL‐induced upregulation of osteogenic markers strongly suggested a direct regulatory role of PRL in PDL and periodontal tissue development.
A comparison of the boomerang wire vascular access management system versus manual compression alone during percutaneous diagnostic and interventional cardiovascular procedures
Authors: Echeverria Angela , Krajcer Zvonimir 
Published: January 2016

Key Points

Vascular closure devices allow for early sheath removal, allowing for earlier patient mobilization
The Boomerang vascular access management system does not alter arterial integrity for future interventions
Access site complications provide significant morbidity in diagnostic and therapeutic interventions
Insulin‐treated diabetes mellitus: An important, actionable risk marker after coronary stenting
Authors: Hillegass William B. 
Published: January 2016

Key Points

Insulin treatment for diabetes is a simple but important risk marker for doubled adjusted death and myocardial infarction rates and tripled stent thrombosis risk after coronary stenting.
Insulin treatment does not predict meaningfully increased major bleeding or additional revascularization procedures after drug eluting coronary stent implantation.
Third generation P2Y12 receptor antagonists substantially lower risk of events in diabetics after stenting with insulin treated diabetics having twice the magnitude of benefit of diabetics not needing insulin.
PCI in failing saphenous vein grafts: DES trumps BMS for the older patient
Authors: Patel Pranav M. , Kern Morton J. 
Published: January 2016

Key Points

PCI to SVG is considered a higher risk intervention requiring clinical judgment, especially in the elderly who may have the oldest and most degenerated SVG conduits.
In a cohort of patients with a median age of 75 years who received PCI to SVG with DES, all‐cause mortality was lower compared to those receiving BMS, and no difference in MI or urgent revascularization was observed to 3 years.
PCI to SVG using DES may be a viable and perhaps superior treatment option for elderly patients.
Saphenous vein graft lesions: Are second‐generation drug‐eluting stents better?
Authors: Kolte Dhaval , Dawn Abbott J. 
Published: January 2016

Key Points

Long‐term outcomes were similar in patients treated with first‐ or second‐generation drug‐eluting stents (DES) for saphenous vein graft (SVG) lesions.
Patients presenting with acute myocardial infarction as a result of occluded SVG may derive some benefit from the use of second‐ versus first‐generation DES
Randomized clinical trials are needed to determine whether newer DES types including those with bioabsorbable polymer or scaffolds offer a distinct advantage in the treatment of SVG lesions.
Heterogeneous Catalysis by Covalent Organic Frameworks (COF): Pd(OAc)2@COF‐300 in Cross‐Coupling Reactions
Authors: Gonçalves Raoni S. B. , de Oliveira Alline B. V. , Sindra Haryadylla C. , Archanjo Bráulio S. , Mendoza Martín E. , Carneiro Leonardo S. A. , Buarque Camilla D. , Esteves Pierre M. 
Published: January 2016

AbstractCOF‐300, an imine‐linked, crystalline, and microporous covalent organic framework, modified by coordination of Pd(OAc)2 to its walls, afforded a hybrid material, Pd(OAc)2@COF‐300, which was used as an efficient heterogeneous catalyst for cross‐coupling reactions. This material showed excellent catalytic activity for the phosphine‐free Suzuki–Miyaura, Heck, and Sonogashira cross‐coupling reactions with low palladium loadings (0.1 mol % Pd). X‐ray photoelectron spectroscopy analysis of the catalyst after the reaction showed that PdII is converted to Pd0, which is trapped within the COFs nanopores. This was confirmed by high‐resolution transmission electron microscopy. Moreover, promising results were obtained using Pd(OAc)2@COF‐300 under continuous‐flow conditions for a Suzuki–Miyaura cross‐coupling reaction.
Fe3O4 Nanoparticles Anchored on Carbon Serve the Dual Role of Catalyst and Magnetically Recoverable Entity in the Aerobic Oxidation of Alcohols
Authors: Geng Longlong , Zheng Bin , Wang Xiang , Zhang Wenxiang , Wu Shujie , Jia Mingjun , Yan Wenfu , Liu Gang 
Published: January 2016

AbstractA composite of Fe3O4 nanoparticles anchored on a carbon support (Fe3O4/C), possessing both superparamagnetism and molecular oxygen activating properties, was prepared by an ammonia‐assisted precipitation method. Fe3O4/C could catalyze the selective oxidation of various benzyl alcohols with air as the oxidant source, and could be easily separated and recycled with an external magnet. The small particle size and the interaction between the Fe3O4 nanoparticles and carbon support endow the Fe3O4/C catalyst with relatively high reducibility. Its oxidation state is easy to change. This intrinsic property of the Fe3O4 nanoparticles could be responsible for the high activity of Fe3O4/C in the aerobic oxidation of alcohols.
Alkaline‐Earth‐Metal‐Catalyzed Thin‐Film Pyrolysis of Cellulose
Authors: Zhu Cheng , Maduskar Saurabh , Paulsen Alex D. , Dauenhauer Paul J. 
Published: January 2016

AbstractThe conversion of lignocellulosic biomass to “bio‐oils” by thermochemical pyrolysis is a promising reactor technology for renewable chemicals and biofuels. Although the fundamental understanding of relevant catalysts within reacting biomass particles is only in its infancy, it is known that inorganic materials naturally present within biomass act as catalysts that limit the yield of bio‐oil and alter the product distribution. In this work, the effect of alkaline earth metals on cellulose pyrolysis chemistry was investigated to determine the catalytic effect on primary (transport‐free) and secondary (diffusion‐limited) reaction pathways. The catalytic materials included homogeneous metal ions Ca2+ and Mg2+ from their inorganic salts, Ca(NO3)2 and Mg(NO3)2, and their corresponding heterogeneous metal oxides, CaO and MgO. Although the oxides had a limited impact on cellulose pyrolysis chemistry, the metal ions altered the secondary reaction pathways of cellulose significantly under diffusion‐limited conditions common to lignocellulosic particles within industrial reactors.
Grafting of Aryl Diazonium, Iodonium, and Sulfonium Salts in Unusual Patterns by Exploiting the Potential Gradient in Bipolar Electrochemistry
Authors: Koefoed Line , Pedersen Steen U. , Daasbjerg Kim 
Published: January 2016

AbstractBipolar electrochemistry makes various kinds of surface modifications possible by appropriately tuning the applied potential difference. In this study aryl diazonium, iodonium, and sulfonium salts are reductively grafted onto gold surfaces using an excess of hydroquinone to set the anode at a fixed potential. In general, aryl diazonium salts are easier to reduce than the corresponding iodonium salts which, again, are more easily reduced than sulfonium salts. For all three salts grafting is observed at the reductive site of the bipolar electrode, but with distinctly different features due to the different reduction potentials required to facilitate the grafting. Depending on the reduction potentials of the salts relative to that of the grafting agent, that is, the aryl radical, it is possible to create movable band‐like grafted areas by diazonium grafting, expanding bands by iodonium grafting, or optically invisible thin bands by sulfonium grafting. From these findings, a relationship between the grafting behavior and the applied potential difference is deduced.
General Polyethyleneimine‐Mediated Synthesis of Ultrathin Hexagonal Co3O4 Nanosheets with Reactive Facets for Lithium‐Ion Batteries
Authors: Wang Bin , Lu Xiao‐Ying , Tang Yuanyuan , Ben Weiwei 
Published: January 2016

AbstractThe front cover artwork is provided by Dr. Xiao‐Ying Lu from Technological and Higher Education Institute of Hong Kong and her collaborators from Hong Kong Applied Science and Technology Research Institute and South University of Science and Technology of China. The image shows facile synthesis of ultrathin hexagonal Co3O4 nanosheets with reactive facets and mesopores by a general polyethyleneimine‐mediated strategy for high‐performance lithium‐ion batteries. Read the full text of the article at 10.1002/celc.201500377.
Beyond the Dimer and Trimer: Tetraspiro[2.1.25.1.29.1.213.13] hexadecane‐1,3,5,7‐tetraone—the Cyclic Tetramer of Carbonylcyclopropane
Authors: Sedenkova Kseniya N. , Averina Elena B. , Grishin Yuri K. , Andriasov Kristian S. , Stepanova Svetlana A. , Roznyatovsky Vitaly A. , Kutateladze Andrei G. , Rybakov Victor B. , Albov Dmitry V. , Kuznetsova Tamara S. , Zefirov Nikolay S. 
Published: January 2016

AbstractTetraspiro[2.1.25.1.29.1.213.13]hexadecane‐1,3,5,7‐tetraone 4, a unique tetraketone containing a cyclooctane core and four spiroannelated cyclopropane moieties, represents the previously unknown cyclotetramer of carbonylcyclopropane. For this purpose oxidation of the parent polyspirocyclic hydrocarbon was examined under various oxidative conditions, and the reactivity of oxidants towards methylene groups of the eight‐membered cycle, activated by adjacent spirocyclopropane rings, was evaluated and contrasted. Whereas the treatment of tetraspirohexadecane with ozone resulted in monooxidation, its reaction with methyl(trifluoromethyl)dioxirane afforded the product of four‐fold oxidation, triketoalcohol 10. Subsequent oxidation of the latter with Dess–Martin periodinane gave the target tetraketone 4.
ChemInform Abstract: Dipolar Cycloaddition‐Based Multicomponent Reactions in Ionic Liquids: A Green, Fully Stereoselective Synthesis of Novel Polycyclic Cage Systems with the Generation of Two New Azaheterocyclic Rings.
Authors: Kumar Raju Suresh , Almansour Abdulrahman I. , Arumugam Natarajan , Menendez J. Carlos , Osman Hasnah , Kumar Raju Ranjith 
Published: January 2016

AbstractBifunctional polycyclic cage structures are synthesized in an ionic liquid by a sustainable microwave‐assisted three‐component [3+2] cycloaddition—annulation of diarylidene‐4‐piperidinones, acenaphthenequinone, and amino acids in good to excellent yields with complete regio‐ and stereoselectivity.
Zeitverhalten der isothermen Reaktivdestillation beim thermischen Cracken und Desoxygenieren von Rapsöl
Authors: Augustin Christian , Sievers Anika , Willner Thomas 
Published: January 2016

AbstractThermisches Cracken von Rapsöl bei der isothermen Reaktivdestillation kann einen Beitrag zur Bereitstellung alternativer flüssiger Brenn‐ und Kraftstoffe leisten. Die zeitliche Änderung der Sumpfphase und des Öl‐Kondensates zeigt einen Brennwertanstieg verursacht durch Desoxygenierung. Die Sumpfphase ist zunehmend thermisch stabiler, begleitet von Polymerisation und Aromatisierung. Auf diese Vorgänge lässt eine Änderung der Jodzahl, des H/C‐Verhältnisses sowie der Viskosität schließen. Die Desoxygenierung des Öl‐Kondensats wird bestätigt durch eine Verringerung der Säurezahl und der detektierten Carbonsäuren.
Selective Liquid Phase Adsorption of Biogenic HMF on Hydrophobic Spherical Activated Carbons
Authors: Schute Kai , Louven Yannik , Detoni Chaline , Rose Marcus 
Published: January 2016

AbstractThe adsorption of biogenic hydroxymethylfurfural and fructose from aqueous phase reaction mixtures on polymer‐based spherical activated carbons was studied. The adsorbents show a high capacity in combination with an outstanding selectivity due to their hydrophobic surface. Equilibrium isotherms and breakthrough curves of single and multicomponent solutions as well as the desorption behavior were measured and modelled accordingly. Overall, the results obtained enable further process development for adsorptive liquid phase separation in future biorefinery processes.
Cooperative or competitive in alliance formation: Alliance patterns with respect to rivals
Authors: Kim Kwang‐Ho 
Published: January 2016

AbstractPremised on the assumption that strategic alliance is a type of competitive action toward rivals, this study explores how a firm uses alliances differently with respect to rivals. I distinguish between two types of alliances that directly involve rivals: alliance with a rival and with a rival's partner. The former and the latter reflect cooperative‐ and competitive‐orientations respectively. Further, I investigate what drives a focal firm to adopt a particular alliance rather than another. The findings indicate that the key consideration explaining different alliance patterns is the resource profiles of focal and rival firms. This study contributes to the literature on competitive dynamics and strategic alliance by suggesting a new approach to integrate interfirm competition and cooperation. Copyright © 2016 ASAC. Published by John Wiley & Sons, Ltd.
Metastatic HPV‐related head and neck squamous cell carcinoma to the lung and mediastinal lymph nodes in aspirated cytology material: A diagnostic pitfall
Authors: Banet Natalie , Rooper Lisa M. , Maleki Zahra 
Published: January 2016

BackgroundAlthough HPV‐related head and neck squamous cell carcinoma (HNSCC) has good prognosis, a small proportion of patients develop distant metastases and have worse outcomes. Such metastases can be particularly difficult to diagnose in the lung and mediastinum, where they show extensive morphologic overlap with primary pulmonary neoplasms. This case series discusses metastatic HPV‐related HNSCC in lung and mediastinal fine needle aspiration (FNA) cytology.MethodsThe cytopathology archives were searched for lung and mediastinal FNA specimens of patients with HNSCC. Specimens were included if either the index FNA or the patient's original HNSCC was positive for HPV by DNA in‐situ hybridization (ISH). Ten such cases were identified. Patient demographics and primary tumor details were tabulated from the electronic medical record. All FNA slides and stains were reviewed.ResultsThe ten patients (mean age of 58.2 years) included 4 smokers. Smears from all cases were hypercellular and hyperchromatic, with focal keratinization in 6/10 (60%). Core biopsies and cell blocks showed basaloid morphology with variable amounts of necrosis. All ten FNAs were diffusely positive for p16 (100%) and 7/9 cases stained (77.8%) were positive for HPV DNA ISH.ConclusionsMetastatic HPV‐related HNSCC to the lung and mediastinal lymph nodes share the characteristic basaloid, minimally keratinizing morphology seen in Primary HNSCC cytology. Poorly differentiated pulmonary squamous cell carcinoma and neuroendocrine carcinomas are the primary differentials. Although primary lung neoplasms are not HPV‐related, p16 positivity can be seen in both squamous cell and small cell lung carcinomas. HPV ISH allows definitive diagnosis of metastatic HPV‐related HNSCC. Diagn. Cytopathol. 2016;44:206–214. © 2016 Wiley Periodicals, Inc.
Analgesic Effect of the Newly Developed S(+)‐Flurbiprofen Plaster on Inflammatory Pain in a Rat Adjuvant‐Induced Arthritis Model
Authors: Sugimoto Masanori , Toda Yoshihisa , Hori Miyuki , Mitani Akiko , Ichihara Takahiro , Sekine Shingo , Hirose Takuya , Endo Hiromi , Futaki Nobuko , Kaku Shinsuke , Otsuka Noboru , Matsumoto Hideo 
Published: January 2016

ABSTRACT

Preclinical Research

This article describes the properties of a novel topical NSAID (Nonsteroidal anti‐inflammatory drug) patch, SFPP (S(+)‐flurbiprofen plaster), containing the potent cyclooxygenase (COX) inhibitor, S(+)‐flurbiprofen (SFP). The present studies were conducted to confirm human COX inhibition and absorption of SFP and to evaluate the analgesic efficacy of SFPP in a rat adjuvant‐induced arthritis (AIA) model. COX inhibition by SFP, ketoprofen and loxoprofen was evaluated using human recombinant COX proteins. Absorption of SFPP, ketoprofen and loxoprofen from patches through rat skin was assessed 24 h after application. The AIA model was induced by injecting Mycobacterium tuberculosis followed 20 days later by the evaluation of the prostaglandin PGE2 content of the inflamed paw and the pain threshold. SFP exhibited more potent inhibitory activity against COX‐1 (IC50 = 8.97 nM) and COX‐2 (IC50 = 2.94 nM) than the other NSAIDs evaluated. Absorption of SFP was 92.9%, greater than that of ketoprofen and loxoprofen from their respective patches. Application of SFPP decreased PGE2 content from 15 min to 6 h and reduced paw hyperalgesia compared with the control, ketoprofen and loxoprofen patches. SFPP showed analgesic efficacy, and was superior to the ketoprofen and loxoprofen patches, which could be through the potent COX inhibitory activity of SFP and greater skin absorption. The results suggested SFPP can be expected to exert analgesic effect clinically. Drug Dev Res 76 : 20–28, 2016. © 2016 Wiley Periodicals, Inc.
The relationship between prior night's sleep and measures of infant imitation
Authors: Konrad Carolin , Herbert Jane S. , Schneider Silvia , Seehagen Sabine 
Published: January 2016

ABSTRACTWe examined whether sleep quality during the night and naps during the day preceding a learning event are related to memory encoding in human infants. Twenty‐four 6‐ and twenty‐four 12‐month‐old infants' natural sleeping behavior was monitored for 24 hr using actigraphy. After the recording period, encoding was assessed using an imitation paradigm. In an initial baseline phase, infants were allowed to interact with the stimulus to assess spontaneous production of any target actions. Infants then watched an experimenter demonstrate a sequence of three target actions and were immediately given the opportunity to reproduce the demonstrated target actions to assess memory encoding. Analyses revealed significant correlations between nighttime sleep quality variables (sleep efficiency, sleep fragmentation) and immediate imitation in 6‐month‐olds, but not in 12‐month‐olds. High sleep quality in the preceding night was thus positively associated with next day's memory encoding in 6‐month‐old infants. © 2016 Wiley Periodicals, Inc. Dev Psychobiol 58: 450–461, 2016.