bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytosolic delivery of anionic cyclic dinucleotide STING agonists with locally supercharged viral capsids
Authors: Huang Paul , Pistono Paige E. , Martin Hannah S. , Fetzer Jennifer L. , Francis Matthew B. 
Published: October 2024

ABSTRACTCyclic dinucleotide (CDN) STING agonists represent a powerful new immunotherapy treatment modality and are a class of nucleotide-based therapies with broad clinical potential. However, they face practical challenges for administration, largely due to their poor pharmacological properties. We report the development of a drug delivery platform for CDNs and other anionic small-molecule drugs using bacteriophage MS2 viral capsids with engineered cationic residues. Relative to viral capsids lacking locally supercharged regions, these assemblies exhibit substantial increases in mammalian cell uptake while avoiding cell toxicity and hemolysis. CDN drugs were covalently attached to the interior capsid surfaces through reductively cleavable disulfide linkers, which allowed for traceless drug release upon cell entry and exposure to reductive cytosolic environments. MS2-mediated CDN delivery into immune cell populations resulted in an approximately 100-fold increase in delivery efficiency compared to free drugs and showed enhanced STING activation as well as downstream cytokine release.
Spatiotemporal characterisation of information coding and exchange in the multiple demand network
Authors: Karimi-Rouzbahani Hamid , Rich Anina N. , Woolgar Alexandra 
Published: October 2024

AbstractThe multiple-demand network (MDN), a brain-wide system with nodes near sensory and higher-order cognitive regions, has been suggested to integrate and exchange task-related information across the brain, supporting cognitive task performance. However, the profile of information coding and the role of each node within this network in information exchange remain unclear. To address this, we combined fMRI and MEG data in a challenging stimulus-response mapping task. Using multivariate pattern analysis (MVPA), we decoded various forms of task information, including coarse and fine stimulus details, motor responses, and stimulus-response mapping rules, across the MDN and visual regions. Early in the task, visual regions responded to large physical differences in stimuli, while later on, fine stimulus information and rules were encoded across the MDN. To assess information exchange between regions, we developed Fusion-RCA, a novel connectivity analysis method based on fMRI-MEG fusion profiles. Our findings revealed significant transfer of fine stimulus information, rules, and responses, but little evidence for the transfer of coarse stimulus information. These results highlight distinct information encoding patterns within MDN nodes and suggest that the anterior cingulate cortex (ACC) plays a key role in distributing task-relevant information. This study offers new insights into the dynamic function of the MDN and introduces Fusion-RCA as a powerful tool for exploring brain-wide information transfer.
Metabolomics survey of uropathogenic bacteria in human urine
Authors: Chan Carly C.Y. , Groves Ryan A. , Rydzak Thomas , Lewis Ian A. 
Published: October 2024

AbstractUrinary tract infections (UTIs) are one of the most prevalent infections in North America and are caused by a diverse range of bacterial species. Although uropathogenesis has been studied extensively in the context of macromolecular interactions, the degree to which metabolism may contribute to infection is unclear. Currently, most of what is known about the metabolic capacity of uropathogens has been derived from genomics, genetic knockout studies or transcriptomic analyses. However, there are currently very little empirical data on the metabolic activity of uropathogens when grown in urine. To address this gap, we conducted a systematic survey of the metabolic activities of eight of the most common uropathogenic bacterial species that collectively represent 99% of uncomplicated UTIs. Liquid chromatography-mass spectrometry (LC-MS) analyses of human urine cultures revealed that uropathogens have four distinct metabolic clades. We generalized these clades as serine consumers (Escherichia coli, Klebsiella pneumoniaeandProteus mirabilis), glutamine consumers (Pseudomonas aeruginosa), amino acid abstainers (Enterococcus faecalisandStreptococcus agalactiae), and amino acid minimalists (Staphylococcus aureusandStaphylococcus saprophyticus). These metabolic classifications can be further subdivided on a species-to-species level. This survey provides a framework to understanding the metabolic activity of the diverse range of uropathogens and how these species use divergent metabolic strategies to occupy the same niche.
Neural mechanisms of the transition from planning to execution in speech production
Authors: Duraivel Suseendrakumar , Rahimpour Shervin , Barth Katrina , Chiang Chia-Han , Wang Charles , Harward Stephen C. , Lad Shivanand P. , Sexton Daniel P. , Friedman Allan H. , Southwell Derek G. , Sinha Saurabh R. , Viventi Jonathan , Cogan Gregory 
Published: October 2024

AbstractThe neural basis of speech production involves the rapid transition from abstract planning of speech units such as syllables and phonemes, to the motor execution of speech sounds. Although a distributed network of brain regions has been implicated in speech production overall, it is unclear how the brain transitions from planning to execution for speech production. Leveraging the high spatio-temporal resolution of intracranial recordings, we find evidence for neural mechanisms that operate in space and time across the prefrontal and premotor cortices to facilitate the transition from planning to execution. During this execution, we show evidence for motor sequencing from neural activity that tracks both phonological units as well as the transition between them, suggesting both discrete elements from planning as well as continuous motor transitions. We demonstrate temporally-resolved neural mechanisms for the transition between planning and execution to facilitate speech production.
Student attitudes toward quantum information science and technology in a high school outreach program
Authors: Darienzo Michele , Kelly Angela M. , Schneble Dominik , Wei Tzu-Chieh 
Published: October 2024

[This paper is part of the Focused Collection in Investigating and Improving Quantum Education through Research.] With the current growth in quantum information science and technology (QIST), there is an increasing need to prepare precollege students for postsecondary QIST study and careers. This mixed methods, explanatory sequential research focused on students’ affective outcomes from a one-week, 25-h summer program for U.S. high school students in grades 10–12. The workshop structure was based upon psychosocial theories of self-determination and planned behavior, where QIST aspirations may be facilitated and viewed as achievable choices if students acquire disciplinary knowledge, self-efficacy, normative expectancy of their capacity in the field, and awareness of vocational roles. The program featured lectures, demonstrations, and hands-on experiences in classical and quantum physics and quantum computing. Students’ attitudes toward QIST (N=77)—including self-efficacy, self-concept, relevance, career aspirations, and perceptions of quantitative fluency—showed improvement with a medium effect size, even though treatment students entered the program with more positive QIST attitudes when compared with a control group of high school physics students (N=65). Postprogram interviews with n=12 participants identified several explanatory themes: (i) Students tended to comprehend classical and quantum topics taught through multiple representations, regardless of whether they had taken physics previously; (ii) students experienced some challenges with mathematics and science concepts that support quantum understanding, yet they revealed a willingness to learn new concepts outside of their comfort zone; (iii) students expressed motivation for pursuing science, technology, engineering, and mathematics and/or quantum-related careers in the future, as well as increased QIST self-concept, largely through understanding the relevance of QIST in solving technological problems; and (iv) students reported increased self-efficacy in understanding QIST topics and performing related tasks. This informal summer program showed promise in promoting positive student attitudes toward QIST, a critical emerging field in advancing technological solutions for global challenges.

Published by the American Physical Society
2024
The “locus continuum” of price increase perception – Is communicating a good cause for price increases always beneficial?
Authors: Pick Doreén , Zielke Stephan 
Published: October 2024

Purpose
Governmental regulations aiming to protect environmental goals often require firms to increase sales prices with negative consequences on price fairness perception. Companies might therefore either justify the price increase by highlighting the good cause (environmental framing) or they could blame the government for the regulation (governmental framing). Firms might also communicate their investments in the relationship to motivate customers to stay. This paper aims to examine the impact of such communication content on price increase fairness perception and switching intention in a contractual service setting.

Design/methodology/approach
This paper first examines the content of 119 price increase letters from electricity suppliers in a qualitative pilot study. The main study then tests our research framework with 552 respondents using a 2 x 2 x 2 between-subjects experimental scenario design (manipulating framing, effort and regret communication).

Findings
Customers perceive governmental framing as fairer than environmental framing. Effort and regret communication by firms weaken or reverse this effect. They reduce customers’ fairness perception for the governmental framing, while regret communication increases it for the environmental framing. However, regret communication also increases switching intention in both framings through a strong direct effect.

Research limitations/implications
Cost-induced price increases are perceived on a “locus continuum” on which reason-framing and relationship investments can shift the consumer perception. Future studies may apply our framework in different industries and contexts.

Practical implications
The results provide guidelines for communicating price increases. Firms should prefer a governmental framing and they should also hesitate to communicate relationship investments, which signal internal locus of the firm, such as effort or regret.

Originality/value
Our results question the naive assumption of general positive effects of environmental framings and relationship investments on customer responses. Based on a new view on attributions of cost-caused price increases, we suggest and find several counterintuitive results. We argue that the framing and relationship investments shift the cause perception of an external cost increase on the attributional locus continuum.
Online business: ambitious daughters and their suspicious career in China?
Authors: Tang Lin , Song Jing 
Published: October 2024

Purpose
Online business can be an attractive career choice but bears gendered implications under China’s market economy. This study aims to examine how highly educated young women negotiate their career choice of online business, given their enhanced career ambitions and the persisting conservative views of their parents. It is to be examined how these factors interact in shaping women’s strategies and commitment to their nonconventional careers.

Design/methodology/approach
The study draws on 23 interviews with 18 women involved in online business. The interviews were coded by themes about how women perceive and make career choices to enter online business, the influence of various motivations (e.g., economic security, decency, autonomy and earning potential) and women’s interactions with their parents under gendered social expectations. The study analyzes and categorizes women’s varied strategies and levels of commitment in taking up online business, an attractive but suspicious career for highly educated young women.

Findings
The study finds that some women chose to conduct online business as a secondary job behind their stable and formal primary job; their compromise under parents’ preferences and social expectations as “compliant daughters,” willingly or forced, coexisted with their persisting interest in online entrepreneurship. Other women, or “self-determined daughters,” embraced online business as their primary job; some benefited from parents’ tolerant views, but others needed to handle the pressure of parental disapproval by hiding or proving their nonconventional career choices worthwhile.

Originality/value
This study speaks to the gendered opportunity-necessity framework of entrepreneurship by illustrating women’s multiple motivations in China’s market reforms and the rising online entrepreneurship dynamics. The findings contextualize women’s career choices in different family dynamics and suggest how social expectations and gender norms are imposed and transformed, with a focus on the shifting gendered concerns of opportunity, security and decency in an era of digital economy.
Dual characteristics of mixed convection flow of three-particle aqueous nanofluid upon a shrinking porous plate
Authors: Roy Nepal Chandra , Hassan Md. Mahmudul , Dinarvand Saeed 
Published: October 2024

Purpose
This study aims to analyze the thermo-hydrodynamic characteristics for the mixed convection boundary layer flow of three-particle aqueous nanofluid on a shrinking porous plate with the influences of thermal radiation and magnetic field.

Design/methodology/approach
The basic equations have been normalized with the help of similarity transformations. The obtained equations have been solved numerically using the shooting method in conjunction with the sixth-order Runge–Kutta technique. Numerical results for the velocity and temperature are illustrated with varying relevant parameters.

Findings
The results reveal that the local drag coefficient increases with higher values of the magnetic field parameter, nanoparticle volume fraction and suction parameter. On the other hand, boosting the radiation parameter and nanoparticle concentration notably enhances heat transfer. Furthermore, it is noted that the suction parameter and magnetic field parameter both lead to an increase in velocity and promote the occurrence of dual solutions within the problem conditions.

Research limitations/implications
The limitations are that the model is appropriate for thermal equilibrium of base fluid and nanoparticles, and constant thermo-physical properties.

Originality/value
To the best of the authors' knowledge, no study has taken an attempt to predict the flow and heat transfer characteristics of unsteady mixed convection ternary hybrid nanofluid flow over a shrinking sheet, particularly under the influence of magnetic field and radiation. The findings obtained here may hold particular significance for those interested in the underlying theoretical and practical implications.
Environmental impacts and BIM: a Portuguese heritage case study
Authors: Sugiyama Gabriel Tomasetto , Rodrigues Maria Fernanda , Rodrigues Hugo 
Published: October 2024

PurposeThis paper aims to compare the possible environmental impacts of different rehabilitation solutions proposed to a case study of a Portuguese vernacular building, bearing in mind the need to search for less impactful practices in accordance with global agendas and, simultaneously, the importance of preserving heritage assets.Design/methodology/approachThe two proposed solutions differ from each other in the selection of materials, varying from the exclusive use of conventional contemporary materials to the use of locally sourced and natural-based materials, considering the Portuguese and local scenario. Results obtained from Life Cycle Assessment procedures are the basis of comparison, which were developed using a Building Information Model (BIM)-integrated analytical plugin.FindingsProducing Heritage Building Information Model (HBIM) models was essential to the analysis, and therefore is a vital part of the used methodology; nevertheless, the modelling of heritage buildings itself plays an important part in preserving heritage assets once it provides precise documenting and managing tools. The results depict variation in values for different indicators in both cases, whose interpretation might need further development to get to deeper conclusions. It has been found that the software tools are still not well adapted to analyse heritage or historic buildings, and therefore adaptations had to take place to overcome these limitations.Originality/valueThe study distinguishes itself by involving the production of HBIM models and the use of BIM-integrated Life Cycle Assessment plugins as well as its analyses, to validate this approach.
Factors affecting coaching in higher education: a meta-synthesis approach
Authors: Dolatkhah Laein Dorry , Andam Reza 
Published: October 2024

PurposeThe purpose of this study was to identify and investigate which factors affect coaching in higher education.Design/methodology/approachThe study employed a qualitative meta-synthesis method. Based on the seven-step method (Sandelowski and Barroso, 2006), articles related to coaching in higher education were searched in five databases between 2012 and 2022. After step-by-step reviews and implementation of the critical evaluation skills program, 34 articles were selected from 1,711 sources.FindingsFollowing the analysis of 58 obtained codes, seven sub-themes and three themes were extracted. The results showed that the key factors affecting coaching in higher education include individual (personal skills, personal attitudes, personal behaviors), structural (environmental contexts, organizational contexts), and managerial factors (planning actions, supporting actions).Practical implicationsThe present study provides a framework for policy-making, implementation, evaluation and development of coaching programs in universities and provides a great help and support for planners and administrators of higher education institutions who want to design and implement coaching for the first time.Originality/valueThe presented factors provide effective information to higher education institutions so that they can offer coaching intervention more efficiently and help them allocate resources in a more efficient and strategic manner.
Encountering risk with resilience for experiences: a case study on tourism in a conflicted tourist destination
Authors: Sufi Tahir , Ranga Bindu , Ranga Indu 
Published: October 2024

Purpose
This paper aims to unravel how tourists balance their novel experiences with risk perceptions, psychological resilience and behavioral intentions. Additionally, it explores how tourists' personalities moderate the relationship between experiences and travel intentions.

Design/methodology/approach
A total of 234 self-administered questionnaires were distributed to a diverse group of tourists who recently explored the Srinagar region to capture their perspectives. The data obtained was analyzed using Smart PLS-SEM.

Findings
This study revealed that the impact of perceived terror risk on behavioral intentions is not statistically significant. Instead, tourists' experiences significantly influence psychological resilience and behavioral intentions. Tourists with higher resilience are inclined to perceive these experiences as aiding in managing negative feelings.

Research limitations/implications
The study's focus is confined to one conflict zone within the country due to research constraints, excluding other areas.

Practical implications
This research provides practical insights for destination management authorities and highlights areas for improvement for tourism service providers and the government in the Srinagar region, as well as other conflict regions. Emphasizing mutual respect between locals and tourists can foster community-based tourism, enhancing the region's appeal and promoting positive intentions for all involved parties.

Social implications
This study examines how local communities in conflict-affected areas adjust to and manage the presence of tourists, with an emphasis on building resilience and support systems. Additionally, it explores how travel decisions and behaviors are influenced by tourists' perceptions of safety and how these perceptions can influence broader societal attitudes toward areas affected by conflict. Evaluating the local population's economic reliance on tourism may result in changed social dynamics, as well as possible exploitation or over-reliance on industry. Promoting mutual understanding and cultural interchange between locals and visitors may have a positive impact on efforts to promote social cohesion and peacebuilding.

Originality/value
This study broadens the scope of the existing literature on destination attributes in conflict zones, offering a unique perspective on the intrinsic features of this issue. The solutions proposed in this study contribute a novel dimension to the current literature.
Better safe than sorry? The effect of asymmetric cost management on firm resilience in manufacturing firms
Authors: Du Ke , Jia Fu , Chen Lujie 
Published: October 2024

PurposeGlobal business practices show that during periods of demand volatility, manufacturing firms often engage in asymmetric cost management (ACM), a behavior in which costs increase faster than they decrease when demand decreases by the same amount. However, whether managing asymmetric costs will enhance or impede firm resilience remains an open question. We aim to investigate the impact of ACM on firm resilience and its boundary conditions.Design/methodology/approachUsing unbalanced panel data of 2,273 Chinese manufacturing listed companies from 2002 to 2021, we conduct an empirical analysis using a double fixed effects model.FindingsOur findings reveal that ACM has a negative effect on firm resilience. This suggests that in coping with external environmental fluctuations, ACM fails to fulfill its expected role effectively. Instead, it manifests as a severe agency problem affecting firm resilience. Further, we find that managerial myopia and digitalization diminish the negative effect, while customer instability exacerbates it.Originality/valueThis study contributes to the literature on the organizational resilience of manufacturing firms by providing an in-depth understanding of cost management and emphasizing the need to consider agency issues carefully when managing asymmetric costs.