bluuebunny/crossref_metadata_2025_split huggingface dataset:
NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells
Authors: Nakaoka Hiroki J. , Hara Toshiro , Yoshino Seiko , Kanamori Akane , Matsui Yusuke , Shimamura Teppei , Sato Hiroshi , Murakami Yoshinori , Seiki Motoharu , Sakamoto Takeharu 
Published: March 2016

AbstractUnlike most cells, cancer cells activate hypoxia inducible factor-1 (HIF-1) to use glycolysis even at normal oxygen levels, or normoxia. Therefore, HIF-1 is an attractive target in cancer therapy. However, the regulation of HIF-1 during normoxia is not well characterised, although Mint3 was recently found to activate HIF-1 in cancer cells and macrophages by suppressing the HIF-1 inhibitor, factor inhibiting HIF-1 (FIH-1). In this study, we analysed Mint3-binding proteins to investigate the mechanism by which Mint3 regulates HIF-1. Yeast two-hybrid screening using Mint3 as bait identified N-terminal EF-hand calcium binding protein 3 (NECAB3) as a novel factor regulating HIF-1 activity via Mint3. NECAB3 bound to the phosphotyrosine-binding domain of Mint3, formed a ternary complex with Mint3 and FIH-1, and co-localised with Mint3 at the Golgi apparatus. Depletion of NECAB3 decreased the expression of HIF-1 target genes and reduced glycolysis in normoxic cancer cells. NECAB3 mutants that binds Mint3 but lacks an intact monooxygenase domain also inhibited HIF-1 activation. Inhibition of NECAB3 in cancer cells by either expressing shRNAs or generating a dominant negative mutant reduced tumourigenicity. Taken together, the data indicate that NECAB3 is a promising new target for cancer therapy.
Insulin Resistance in Human iPS Cells Reduces Mitochondrial Size and Function
Authors: Burkart Alison M. , Tan Kelly , Warren Laura , Iovino Salvatore , Hughes Katelyn J. , Kahn C. Ronald , Patti Mary-Elizabeth 
Published: March 2016

AbstractInsulin resistance, a critical component of type 2 diabetes (T2D), precedes and predicts T2D onset. T2D is also associated with mitochondrial dysfunction. To define the cause-effect relationship between insulin resistance and mitochondrial dysfunction, we compared mitochondrial metabolism in induced pluripotent stem cells (iPSC) from 5 healthy individuals and 4 patients with genetic insulin resistance due to insulin receptor mutations. Insulin-resistant iPSC had increased mitochondrial number and decreased mitochondrial size. Mitochondrial oxidative function was impaired, with decreased citrate synthase activity and spare respiratory capacity. Simultaneously, expression of multiple glycolytic enzymes was decreased, while lactate production increased 80%. These perturbations were accompanied by an increase in ADP/ATP ratio and 3-fold increase in AMPK activity, indicating energetic stress. Insulin-resistant iPSC also showed reduced catalase activity and increased susceptibility to oxidative stress. Thus, insulin resistance can lead to mitochondrial dysfunction with reduced mitochondrial size, oxidative activity and energy production.
Physical connections between different SSVEP neural networks
Authors: Wu Zhenghua 
Published: March 2016

AbstractThis work investigates the mechanism of the Steady-State Visual Evoked Potential (SSVEP). One theory suggests that different SSVEP neural networks exist whose strongest response are located in different frequency bands. This theory is based on the fact that there are similar SSVEP frequency-amplitude response curves in these bands. Previous studies that employed simultaneous stimuli of different frequencies illustrated that the distribution of these networks were similar, but did not discuss the physical connection between them. By comparing the SSVEP power and distribution under a single-eye stimulus and a simultaneous, dual-eye stimulus, this work demonstrates that the distributions of different SSVEP neural networks are similar to each other and that there should be physical overlapping between them. According to the band-pass filter theory in a signal transferring channel, which we propose in this work for the first time, there are different amounts of neurons that are involved under repetitive stimuli of different frequencies and that the response intensity of each neuron is similar to each other so that the total response (i.e., the SSVEP) that is observed from the scalp is different.
Role of surgery in patients with focally progressive gastrointestinal stromal tumors resistant to imatinib
Authors: Gao Xiaodong , Xue Anwei , Fang Yong , Shu Ping , Ling Jiaqian , Qin Jing , Hou Yingyong , Shen Kuntang , Sun Yihong , Qin Xinyu 
Published: March 2016

AbstractThe benefits of surgery for focally progressive gastrointestinal stromal tumor (GIST) during imatinib therapy are still in discussion. The aim of this study was to compare the outcomes of surgical resection of progressive lesions following tyrosine kinase inhibitor (TKI) therapy (S group) or TKI therapy alone (NS group) in GIST patients. We retrospectively investigated 57 patients with focally progressive GIST during imatinib therapy who were treated in Zhongshan hospital, Fudan University. Progression-free survival (PFS) and overall survival (OS) in the S group were significantly longer than those in the NS group. Among S group, the patients with R0 resection showed longer PFS than R2 resection; however, no difference was found between these two groups. Moreover, PFS and OS were not different in the NS-S group compared with S group. On multivariate analysis, surgery is an independent prognostic factor for longer PFS and OS. Our study supports the decision of treating GIST patients who were focally resistant to imatinib with surgery resection based on its benefit.
A Web Server and Mobile App for Computing Hemolytic Potency of Peptides
Authors: Chaudhary Kumardeep , Kumar Ritesh , Singh Sandeep , Tuknait Abhishek , Gautam Ankur , Mathur Deepika , Anand Priya , Varshney Grish C. , Raghava Gajendra P. S. 
Published: March 2016

AbstractNumerous therapeutic peptides do not enter the clinical trials just because of their high hemolytic activity. Recently, we developed a database, Hemolytik, for maintaining experimentally validated hemolytic and non-hemolytic peptides. The present study describes a web server and mobile app developed for predicting and screening of peptides having hemolytic potency. Firstly, we generated a dataset HemoPI-1 that contains 552 hemolytic peptides extracted from Hemolytik database and 552 random non-hemolytic peptides (from Swiss-Prot). The sequence analysis of these peptides revealed that certain residues (e.g., L, K, F, W) and motifs (e.g., “FKK”, “LKL”, “KKLL”, “KWK”, “VLK”, “CYCR”, “CRR”, “RFC”, “RRR”, “LKKL”) are more abundant in hemolytic peptides. Therefore, we developed models for discriminating hemolytic and non-hemolytic peptides using various machine learning techniques and achieved more than 95% accuracy. We also developed models for discriminating peptides having high and low hemolytic potential on different datasets called HemoPI-2 and HemoPI-3. In order to serve the scientific community, we developed a web server, mobile app and JAVA-based standalone software (http://crdd.osdd.net/raghava/hemopi/).
Strain-induced water dissociation on supported ultrathin oxide films
Authors: Song Zhenjun , Fan Jing , Xu Hu 
Published: March 2016

AbstractControlling the dissociation of single water molecule on an insulating surface plays a crucial role in many catalytic reactions. In this work, we have identified the enhanced chemical reactivity of ultrathin MgO(100) films deposited on Mo(100) substrate that causes water dissociation. We reveal that the ability to split water on insulating surface closely depends on the lattice mismatch between ultrathin films and the underlying substrate, and substrate-induced in-plane tensile strain dramatically results in water dissociation on MgO(100). Three dissociative adsorption configurations of water with lower energy are predicted, and the structural transition going from molecular form to dissociative form is almost barrierless. Our results provide an effective avenue to achieve water dissociation at the single-molecule level and shed light on how to tune the chemical reactions of insulating surfaces by choosing the suitable substrates.
Occurrence and removal of phenolic endocrine disrupting chemicals in the water treatment processes
Authors: Lv Xuemin , Xiao Sanhua , Zhang Gang , Jiang Pu , Tang Fei 
Published: March 2016

AbstractThis paper evaluated the occurrence and removal efficiency of four selected phenolic endocrine disrupting chemicals (bisphenol A (BPA), octylphenol (OP), nonylphenol (NP) and diethylstilbestrol (DES)) in two drinking waterworks in Jiangsu province which take source water from Taihu Lake. The recombined yeast estrogen screen (YES) and liquid chromatography tandem mass spectrometry (LC-MS/MS) were applied to assess the estrogenicity and detect the estrogens in the samples. The estrogen equivalents (EEQs) ranged from nd (not detected) to 2.96 ng/L, and the estrogenic activities decreased along the processes. Among the 32 samples, DES prevailed in all samples, with concentrations ranging 1.46–12.0 ng/L, BPA, OP and NP were partially detected, with concentrations ranging from nd to 17.73 ng/L, nd to 0.49 ng/L and nd to 3.27 ng/L, respectively. DES was found to be the main contributor to the estrogenicity (99.06%), followed by NP (0.62%), OP (0.23%) and BPA (0.09%). From the observation of treatment efficiency, the advanced treatment processes presented much higher removal ratio in reducing DES, the biodegradation played an important role in removing BPA, ozonation and pre-oxidation showed an effective removal on all the four estrogens; while the conventional ones can also reduce all the four estrogens.
What can rodent models tell us about apathy and associated neuropsychiatric symptoms in Parkinson’s disease?
Authors: Magnard R , Vachez Y , Carcenac C , Krack P , David O , Savasta M , Boulet S , Carnicella S 
Published: March 2016

AbstractIn addition to classical motor symptoms, Parkinson’s disease (PD) patients display incapacitating neuropsychiatric manifestations, such as apathy, anhedonia, depression and anxiety. These hitherto generally neglected non-motor symptoms, have gained increasing interest in medical and scientific communities over the last decade because of the extent of their negative impact on PD patients’ quality of life. Although recent clinical and functional imaging studies have provided useful information, the pathophysiology of apathy and associated affective impairments remains elusive. Our aim in this review is to summarize and discuss recent advances in the development of rodent models of PD-related neuropsychiatric symptoms using neurotoxin lesion-based approaches. The data collected suggest that bilateral and partial lesions of the nigrostriatal system aimed at inducing reliable neuropsychiatric-like deficits while avoiding severe motor impairments that may interfere with behavioral evaluation, is a more selective and efficient strategy than medial forebrain bundle lesions. Moreover, of all the different classes of pharmacological agents, D2/D3 receptor agonists such as pramipexole appear to be the most efficient treatment for the wide range of behavioral deficits induced by dopaminergic lesions. Lesion-based rodent models, therefore, appear to be relevant tools for studying the pathophysiology of the non-motor symptoms of PD. Data accumulated so far confirm the causative role of dopaminergic depletion, especially in the nigrostriatal system, in the development of behavioral impairments related to apathy, depression and anxiety. They also put forward D2/D3 receptors as potential targets for the treatment of such neuropsychiatric symptoms in PD.
Gain enhancement of implanted antenna using lens and parasitic ring
Authors: Alamri S. , AlAmoudi A. , Langley R. 
Published: March 2016

This Letter presents methods for improving the gain and efficiency of a microstrip patch antenna operating at 2.45 GHz implanted in the fat layer of the human body. Simulations using CST Microwave Studio are verified by measurements carried out in an anechoic chamber using a cut of fresh pork meat which has close electromagnetic characteristics to the human body. Using a glass lens and a parasitic ring placed on the skin the implanted antenna gain can be enhanced by almost 8.5 dB.
4 × 4 matrix keyboard interface circuit achieved by single port lines
Authors: Lin Zhiqi , Shi Xinrong 
Published: March 2016

It is usual that eight pins is used to connect the single‐chip with 4 × 4 matrix keyboard by the method of scanning. A method without any other active element is raised. Only one pin, four capacitors and four resistances are used to connect the single‐chip with 4 × 4 matrix keyboard without any other chip and active element.
Wireless power transfer using a field‐enhancing coil and a small‐sized receiver with low coupling coefficient
Authors: Mao Shitong , Zhang Jiantao , Song Kai , Wei Guo , Zhu Chunbo 
Published: March 2016

This study investigates the near‐field wireless power transfer system with small‐sized receiver. The receiver is placed at a relatively long distance from the power emitter. The coupling between the receiver and the emitter is very weak, which results in low‐power delivery and efficiency. A passive resonance coil, which enhances the field, is added to the emitter to address these problems. The necessary coupled‐mode theory is developed and combined with optimal operation of the class‐E amplifier. The impact of the enhancing coil (EX) on the energy distribution is discussed from the perspective of energy amplification between the source coil and the EX. The point of the maximum energy of the EX is found and necessary conditions for improving transfer efficiency of such structure are proposed. An experimental setup operating at a frequency of 300 kHz was built using the EX concept. The maximum secondary magnetic field generated by the EX was established and used to wirelessly power a 4 cm receiver with more than 40 mW at 0.5 m. The structure is also compared with a similar system without an EX. Test results show that the inclusion of the EX in the emitter strengthens the spatial magnetic field and improves the transmission efficiency.
Time-variable gravity fields derived from GPS tracking of Swarm
Authors: Bezděk Aleš , Sebera Josef , Teixeira da Encarnação João , Klokočník Jaroslav 
Published: March 2016

Abstract
Since 2002 Gravity Recovery and Climate Experiment (GRACE) provides monthly gravity fields from K-band ranging (KBR) between two GRACE satellites. These KBR gravity monthlies have enabled the global observation of time-varying Earth mass signal at a regional scale (about 400 km resolution). Apart from KBR, monthly gravity solutions can be computed from onboard GPS data. The newly reprocessed GPS monthlies from 13 yr of GRACE data are shown to yield correct time-variable gravity signal (seasonality, trends, interannual variations) at a spatial resolution of 1300 km (harmonic degree 15). We show that GPS fields from new Swarm mission are of similar quality as GRACE GPS monthlies. Thus, Swarm GPS monthlies represent new and independent source of information on time-variable gravity, and, although with lower resolution and accuracy, they can be used for its monitoring, particularly if GRACE KBR/GPS data become unavailable before GRACE Follow-On is launched (2017 August).
Identification of Circulating MicroRNAs in Biliary Atresia by Next‐Generation Sequencing
Authors: Peng Xiaofang , Yang Liyuan , Liu Haiying , Pang Shuyin , Chen Yihao , Fu Jie , Chen Yi , Wen Zhe , Zhang Ruizhong , Zhu Bing , Yu Jiakang , Invernizzi Pietro 
Published: March 2016

ABSTRACTObjectives:Biliary atresia (BA) is an idiopathic neonatal liver disease, characterized by inflammatory and fibrotic obliteration of extrahepatic bile ducts. Therefore, reliable methods for noninvasive diagnosis are needed. The present study aimed to analyze circulating microRNAs (miRNAs) in patients with BA using next‐generation sequencing for identifying novel diagnostic biomarkers.Methods:An initial screening of miRNAs in plasma from patients with BA and healthy controls (HCs) was performed on an Illumina next‐generation sequencing platform. Differential miRNAs were validated by quantitative real‐time polymerase chain reaction (qPCR). Target genes and related signal transduction pathways of differential miRNAs were predicted by online software.Results:In total, 146 differential miRNAs were identified by deep sequencing. Fifteen miRNAs with read counts >1000, that included 7 upregulated and 8 downregulated miRNAs, were predicted to be associated with liver fibrosis, biliary differentiation, and bile duct development. Of these, 6 miRNAs with read counts >5000 were analyzed by qPCR on an independent sample set comprising 44 patients with BA, 20 cholestatic disease controls, and 20 HCs. Two upregulated miRNAs (miR‐122‐5p, miR‐100‐5p) and 2 downregulated miRNAs (miR‐140‐3p, miR‐126‐3p) were confirmed by individual qPCR. Only miR‐140‐3p was significantly different from controls (P < 0.05), yielding an area under receiver operating characteristic curve of 0.75 with sensitivity of 66.7% and specificity of 79.1% at optimal threshold.Conclusions:Our findings indicate that patients with BA exhibit a distinct profile of circulating miRNAs and that plasma miR‐140‐3p may be a promising diagnostic biomarker for this disease.
Positive selection on a regulatory insertion-deletion polymorphism inFADS2influences apparent endogenous synthesis of arachidonic acid
Authors: Kothapalli Kumar S.D. , Ye Kaixiong , Gadgil Maithili S. , Carlson Susan E. , O’Brien Kimberly O. , Zhang Ji Yao , Park Hui Gyu , Ojukwu Kinsley , Zou James , Hyon Stephanie S. , Joshi Kalpana S. , Keinan Alon , Brenna J. Thomas 
Published: March 2016

AbstractLong chain polyunsaturated fatty acids (LCPUFA) are bioactive components of membrane phospholipids and serve as substrates for signaling molecules. LCPUFA can be obtained directly from animal foods or synthesized endogenously from 18 carbon precursors via theFADS2coded enzyme. Vegans rely almost exclusively on endogenous synthesis to generate LCPUFA and we hypothesized that an adaptive genetic polymorphism would confer advantage. The rs66698963 polymorphism, a 22 bp insertion-deletion withinFADS2, is associated with basalFADS1expression, and coordinated induction ofFADS1andFADS2 in vitro. Here we determined rs66698963 genotype frequencies from 234 individuals of a primarily vegetarian Indian population and 311 individuals from the U.S. A much higher I/I genotype frequency was found in Indians (68%) than in the U.S. (18%). Analysis using 1000 Genomes Project data confirmed our observation, revealing a global I/I genotype of 70% in South Asians, 53% in Africans, 29% in East Asians, and 17% in Europeans. Tests based on population divergence, site frequency spectrum and long-range haplotype consistently point to positive selection encompassing rs66698963 in South Asian, African and some East Asian populations. Basal plasma phospholipid arachidonic acid status was 8% greater in I/I compared to D/D individuals. The biochemical pathway product-precursor difference, arachidonic acid minus linoleic acid, was 31% and 13% greater for I/I and I/D compared to D/D, respectively. Our study is consistent with previousin vitrodata suggesting that the insertion allele enhances n-6 LCPUFA synthesis and may confer an adaptive advantage in South Asians because of the traditional plant-based diet practice.
A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants
Authors: Jia Ning , Liu Xiaomin , Gao Hongbo 
Published: March 2016

AbstractPlant meristem cells divide and differentiate in a spatially and temporally regulated manner, ultimately giving rise to organs. In this study, we isolated the Arabidopsis jing he sheng 1 (jhs1) mutant, which exhibited retarded growth, an abnormal pattern of meristem cell division and differentiation, and morphological defects such as fasciation, an irregular arrangement of siliques, and short roots. We identified JHS1 as a homolog of human and yeast DNA Replication Helicase/Nuclease2, which is known to be involved in DNA replication and damage repair. JHS1 is strongly expressed in the meristem of Arabidopsis. The jhs1 mutant was sensitive to DNA damage stress and had an increased DNA damage response, including increased expression of genes involved in DNA damage repair and cell cycle regulation, and a higher frequency of homologous recombination. In the meristem of the mutant plants, cell cycle progression was delayed at the G2 or late S phase and genes essential for meristem maintenance were misregulated. These results suggest that JHS1 plays an important role in DNA replication and damage repair, meristem maintenance, and development in plants.
The role of absorptive and desorptive capacity (ACDC) in sustainable supply management
Authors: Meinlschmidt Jan , Foerstl Kai , Kirchoff Jon F 
Published: March 2016

Purpose
– Sustainable supply management (SSM) has attracted considerable attention from researchers in recent years concentrating on how firms develop and use SSM capabilities to meet stakeholder demands. Acquiring and sharing sustainability knowledge with suppliers have been identified as critical success factors of SSM. The purpose of this paper is to identify the mechanisms that allow firms to effectively acquire and share sustainability-related knowledge with suppliers and how these knowledge generation and desorption mechanisms support the evolution of firm SSM capabilities.

Design/methodology/approach
– To address the research purpose, four longitudinal case studies, two industry leaders in SSM and two industry followers, were conducted at multiple consecutive points in time between 2008 and 2013.

Findings
– The results indicate which mechanisms constitute a sustainability-related absorptive and desorptive capacity and how they support SSM. Thereby, this research explains which mechanisms support firms to acquire sustainability knowledge, assimilate and exploit it and also share it with their suppliers over time.

Research limitations/implications
– This research sheds light on the development and refinement of SSM capabilities by studying the explorative and exploitative learning cycles within focal buying firms taking place over time. Findings indicate a multiplicity in applying absorptive capacity- and desorptive capacity-related mechanisms yields an ambidextrous ability to simultaneously exploit existing knowledge through incremental SSM improvements and explore new SSM knowledge for more radical refinements of SSM capabilities.

Practical implications
– The results provide a blueprint for firms, especially for sustainability followers, seeking to develop effective SSM capabilities. Furthermore, the results explain which mechanisms support firms to acquire, assimilate and exploit sustainability knowledge and also to share it with their suppliers.

Originality/value
– SSM knowledge acquisition, assimilation, exploitation and sharing takes place over time in focal buying firms. This ongoing process helps explain how an SSM capability development and refinement is manifested in both leaders and followers.