bluuebunny/crossref_metadata_2025_split huggingface dataset:
A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
Authors: Royle Kate E. , Polizzi Karen 
Published: November 2017

AbstractAlthough recent advances in E. coli self-assembly have greatly simplified cloning, these have not yet been harnessed for the high-throughput generation of expression strains in the early research and discovery phases of biopharmaceutical production. Here, we have refined the technique and incorporated it into a streamlined workflow for the generation of Pichia pastoris expression strains, reducing the timeline by a third and removing the reliance on DNA editing enzymes, which often require troubleshooting and increase costs. We have validated the workflow by cloning 24 human proteins of biopharmaceutical value, either as direct therapeutics or as research targets, which span a continuous range in size and GC content. This includes demonstrating the applicability of the workflow to three-part assemblies for a monoclonal antibody and its single-chain antibody fragments derivatives. This workflow should enable future research into recombinant protein production by P. pastoris and a synthetic biology approach to this industrial host.
Porphyromonas gingivalis lipopolysaccharides act exclusively through TLR4 with a resilience between mouse and human
Authors: Nativel Brice , Couret David , Giraud Pierre , Meilhac Olivier , d’Hellencourt Christian Lefebvre , Viranaïcken Wildriss , Da Silva Christine Robert 
Published: November 2017

AbstractPorphyromonas gingivalis is a key bacterium in chronic periodontitis, which is associated with several chronic inflammatory diseases. Lipopolysaccharides from P. gingivalis (Pg LPS) can activate multiple cell types via the production of pro-inflammatory cytokines. The receptors for Pg LPS have initially been reported as TLR2, contrasting with the well-studied TLR4 receptor for E. coli LPS; this observation remains controversial since synthetic Pg lipid A activates TLR4 but not TLR2. Despite this observation, the dogma of Pg LPS-mediated TLR2 activation remains the basis of many hypotheses and result interpretations. In the present work, we aimed at determining whether TLR4 or TLR2, or both, mediate Pg LPS pro-inflammatory activity using Pg LPS with different grades of purity, instead of synthetic lipid A from Pg LPS. Here we show that Pg LPS 1) acts exclusively through TLR4, and 2) are differently recognized by mouse and human TLR4 both in vitro and in vivo. Taken together, our results suggest that Pg LPS activity is mediated exclusively through TLR4 and only weakly induces proinflammatory cytokine secretion in mouse models. Caution should be taken when extrapolating data from mouse systems exposed to Pg or Pg LPS to humans.
Embedded AGN and star formation in the central 80 pc of IC 3639
Authors: Fernández-Ontiveros J. A. , Tristram K. R. W. , Hönig S. , Gandhi P. , Weigelt G. 
Published: November 2017

Aims. Our goal is to probe the inner structure and the nature of the mid-IR emission in the active galaxy IC 3639, which hosts a Seyfert 2 nucleus and shows signatures of strong star-forming activity.
Methods. We used interferometric observations in the N-band with VLTI/MIDI to resolve the mid-IR emission of this nucleus. The origin of the nuclear infrared emission is determined from: (1) the comparison of the correlated fluxes from VLTI/MIDI with the fluxes measured at subarcsecond resolution (VLT/VISIR, VLT/ISAAC); (2) diagnostics based on IR fine-structure line ratios, the IR continuum emission, IR bands produced by polycyclic aromatic hydrocarbons (PAH) and silicates; and (3) the high-angular resolution spectral energy distribution.
Results. A large fraction of the total mid-IR emission of IC 3639 is produced in the innermost ≲80 pc with only ~1% of the total luminosity released in the UV/optical range. The unresolved flux of IC 3639 is 90 ± 20 mJy at 10.5 μm, measured with three different baselines in VLTI (UT1–UT2, UT3–UT4, and UT2–UT3; 46–58 m), making this the faintest measurement so far achieved with mid-IR interferometry. The correlated flux is a factor of 3–4 times fainter than the VLT/VISIR total flux measurement. The observations suggest that most of the mid-IR emission has its origin on spatial scales between 10 and 80 pc (40–340 mas). The emission confined within the inner 80 pc is either dominated by a starburst component or by the AGN core. The brightness distribution could be reproduced by a single component associated with the AGN, although this scenario would imply a very extended dust distribution when compared to other nearby Seyfert galaxies detected with MIDI. The extended component could also be associated with polar dust emission, that is, with a dusty wind blown by the AGN. However, a mixed contribution dominated by the star formation component over the AGN is favoured by the diagnostics based on ratios of IR fine-structure emission lines, the shape of the IR continuum, and the PAH and silicate bands.
Conclusions. A composite AGN-starburst scenario is able to explain both the mid-IR brightness distribution and the IR spectral properties observed in the nucleus of IC 3639. The nuclear starburst would dominate the mid-IR emission and the ionisation of low-excitation lines (e.g. [Ne II]12.8 μm) with a net contribution of ~70%. The AGN accounts for the remaining ~30% of the mid-IR flux, ascribed to the unresolved component in the MIDI observations, and the ionisation of high-excitation lines (e.g. [Ne V]14.3 μm and [O Iv]25.9 μm).
Premenstrual Syndrome Diagnosis: A Comparative Study between the Daily Record of Severity of Problems (DRSP) and the Premenstrual Symptoms Screening Tool (PSST)
Authors: Henz Aline , Ferreira Charles Francisco , Oderich Carolina Leão , Gallon Carin Weirich , Castro Juliana Rintondale Sodré de , Conzatti Maiara , Fleck Marcelo Pio de Almeida , Wender Maria Celeste Osório 
Published: November 2017

Objective To validate the premenstrual symptoms screening tool (PSST) in relation to the daily record of severity of problems (DRSP) for premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) diagnoses.
Methods A cross-sectional study with 127 women (20–45 years) with PMS complaints. The women were evaluated in terms of weight, height and body mass index (BMI). After using the primary care evaluation of mental disorders (PRIME-MD) questionnaire to exclude the diagnosis of depression, the PSST was completed and the women were instructed to fill out the DRSP for two consecutive menstrual cycles. The agreement between the two questionnaires was assessed by the Kappa (k) and the prevalence-adjusted, bias-adjusted kappa (PABAK) values.
Results Two-hundred and eighty-two women met the eligibility criteria and answered the PSST. The DRSP was completed for two cycles by 127 women. The percentages of women with PMS and PMDD diagnoses by the DRSP were 74.8% and 3.9% respectively; by PSST, the percentages were41.7% and 34.6% respectively. The number of patients considered “normal” (with symptoms below the threshold for the diagnosis of PMS) was similar in both questionnaires. There was no agreement (Kappa = 0.12) in the results of PMS/ PMDD diagnosis (the PABAK coefficient confirmed this result = 0.39). The PSST had a high sensitivity (79%) and a low specificity (33.3%) for PMS/PMDD diagnosis.
Conclusion The PSST should be considered a diagnostic screening tool. Positive PMS/PMDD cases by PSST should be further evaluated by DRSP to confirm the diagnosis.
Verapamil (VER) Enhances the Cytotoxic Effects of Docetaxel and Vinblastine Combined Therapy Against Non-Small Cell Lung Cancer Cell Lines
Authors: Jaferian Soleiman , Soleymaninejad Maryam , Daraee Hadis 
Published: November 2017

AbstractLung cancer is one of the foremost tumor-associated cause of death in the world. Most of the patients with NSCLC possesses an advanced disease at diagnosis, and are thus probable subject for systemic therapy. This study aims to evaluate the cytotoxicity of vinblastine and docetaxel combined therapy for the treatment of NSCLC, as well as verapamil (VER) enhancement of the combined therapy. We conducted P-glycoprotein (P-gp) gene expression, protein expression with RT-PCR and western blot respectively, apoptotic response of the combined therapy with VER is also determined using DAPI staining (%). Result of DAPI staining confirmed combination therapy promotes cell apoptosis to greater extent as compared to each drug alone. Real-time RT-PCR analysis revealed that mdr-1 expression level increased 6 fold with docetaxel (40 nM) and 2 fold with vinblastine (30 nM) after 24 h (p<0.001). Consequently, combination therapy reduced drug-induced up-regulation of mdr-1 significantly (p<0.05). VER with the drug combination increased P-gp expression (p<0.05). These data provide evidence showing combined therapy is a better approach to improve the efficacy of chemotherapy and decreasing drug resistance.
Hörverlust und Sprachverstehen im Alter
Authors: von Gablenz Petra , Holube Inga 
Published: November 2017

ZusammenfassungDas Verstehen von Sprache bei Hintergrundgeräuschen bereitet vielen älteren Menschen Probleme. In der klinischen Praxis wird diese Qualität
des Hörvermögens durch Sprachtests im Störgeräusch untersucht. Die Studie HÖRSTAT setzte den Göttinger Satztest (GÖSA) im Störgeräusch
zusätzlich zur Tonaudiometrie und einer Befragung bei einer Zufallsstichprobe aus der Allgemeinbevölkerung ein. Wie erwartet nahm der Anteil
Schwerhörender ab ca. 60 Jahren stark zu. Bei Ausschluss sehr hoher Hörverluste waren im Alter von 60–85 Jahren jedoch 68 % von 553
Probanden nach dem WHO-Kriterium als normalhörend einzustufen. Von diesen erreichte im GÖSA allerdings nur jeder Dritte annähernd das
Sprachverstehen im Störgeräusch junger Normalhörender. Die Selbsteinschätzung des Hörvermögens folgte dabei nur eingeschränkt der messbaren
Verschlechterung. Ab einem Alter von ca. 70 Jahren wurden die eigenen Hör-Fähigkeiten eher über- und die Defizite unterschätzt.
Parallel magnetic field suppresses dissipation in superconducting nanostrips
Authors: Wang Yong-Lei , Glatz Andreas , Kimmel Gregory J. , Aranson Igor S. , Thoutam Laxman R. , Xiao Zhi-Li , Berdiyorov Golibjon R. , Peeters François M. , Crabtree George W. , Kwok Wai-Kwong 
Published: November 2017

Significance
Absolute zero resistance of superconducting materials is difficult to achieve in practice due to the motion of microscopic Abrikosov vortices, especially when external currents are applied. Even a partial resistance reduction via vortex immobilization by microscopic material imperfections is the holy grail of superconductivity research. It is commonly believed that the dissipation increases with applied magnetic field since the number of vortices increases as well. Through the example of molybdenum–germanium superconducting nanostrips, we show that resistive losses due to vortex motion can actually be decreased by applying an increasing applied magnetic field parallel to the current. This surprising recovery of superconductivity is achieved through “vortex crowding”: The increased number of vortices impedes their mutual motion, resulting in straight, untwisted vortices.
Skill networks and measures of complex human capital
Authors: Anderson Katharine A. 
Published: November 2017

Significance
The relationship between worker human capital and wages is a question of considerable economic interest. Skills are usually characterized using a one-dimensional measure, such as years of training. However, in knowledge-based production, the interaction between a worker’s skills is also important. Here, we propose a network-based method for characterizing worker skill sets. We construct a human capital network, wherein nodes are skills and two skills are connected if a worker has both or both are required for the same job. We then illustrate the method by analyzing an online freelance labor market, showing that workers with diverse skills earn higher wages and that those who use their diverse skills in combination earn the highest wages of all.
Nutritional and greenhouse gas impacts of removing animals from US agriculture
Authors: White Robin R. , Hall Mary Beth 
Published: November 2017

SignificanceUS agriculture was modeled to determine impacts of removing farmed animals on food supply adequacy and greenhouse gas (GHG) emissions. The modeled system without animals increased total food production (23%), altered foods available for domestic consumption, and decreased agricultural US GHGs (28%), but only reduced total US GHG by 2.6 percentage units. Compared with systems with animals, diets formulated for the US population in the plants-only systems had greater excess of dietary energy and resulted in a greater number of deficiencies in essential nutrients. The results give insights into why decisions on modifications to agricultural systems must be made based on a description of direct and indirect effects of change and on a dietary, rather than an individual nutrient, basis.
Ecosystem management and land conservation can substantially contribute to California’s climate mitigation goals
Authors: Cameron D. Richard , Marvin David C. , Remucal Jonathan M. , Passero Michelle C. 
Published: November 2017

Significance
Combatting climate change will require using all available tools, especially those that contribute to other societal and economic goals, such as natural resource protection and energy security. Conserving and managing natural and agricultural lands to retain and absorb greenhouse gasses (GHGs) are tools that have not been widely integrated into climate policy. Our analysis provides a quantification of potential climate benefits from multiple land-based activities for a jurisdiction with an emissions reduction target (up to 13.3% of the cumulative reductions needed to meet the 2050 target, or nearly three-fourths of a billion metric tons of GHGs). This approach provides a model that other jurisdictions can use to evaluate emissions reductions that might be achieved from conserving and restoring natural lands.
Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein
Authors: Leyme Anthony , Marivin Arthur , Maziarz Marcin , DiGiacomo Vincent , Papakonstantinou Maria P. , Patel Prachi P. , Blanco-Canosa Juan B. , Walawalkar Isha A. , Rodriguez-Davila Gonzalo , Dominguez Isabel , Garcia-Marcos Mikel 
Published: November 2017

Significance
Dysregulation of signaling via heterotrimeric G proteins leads to pathogenesis. Thus, developing an efficient armamentarium to study G protein regulation is crucial for understanding the molecular basis of disease. The classical view of G protein activation as an exclusive function of G protein-coupled receptors has been challenged by the discovery of nonreceptor G protein activators. Dysregulation of a family of such nonreceptor activators has been linked to human disorders like cancer or birth defects, but the underlying mechanisms remain poorly understood due to the lack of experimental tools. Here, we use protein engineering to rationally design a genetically encoded inhibitor of these G protein activators and demonstrate its usefulness to block aberrant signaling in cancer cells and abrogate developmental malformations in animal embryos.
Role of estrogen receptor beta in neural differentiation of mouse embryonic stem cells
Authors: Varshney Mukesh K. , Inzunza José , Lupu Diana , Ganapathy Vaidheeswaran , Antonson Per , Rüegg Joëlle , Nalvarte Ivan , Gustafsson Jan-Åke 
Published: November 2017

Significance
Controlling the proliferation and proper fate acquisition of pluripotent stem cells is a major challenge in regenerative therapies today. Our study reveals that the estrogen receptor beta (ERβ) is an important factor in maintaining the neuroepithelial and midbrain stem cell pools by repressing proliferation and early nonneuronal fate acquisition. We report on the factors that underlie these effects of ERβ. Further, we report that ERβ facilitates midbrain dopaminergic fate and function. The data presented in this study suggest that ERβ is a factor to be considered in designing regenerative therapies for example neurodegenerative diseases such as Parkinson’s disease.
ASEAN think tanks, policy change and economic cooperation: from the Asian financial crisis to the global financial crisis
Authors: Zimmerman Erin , Stone Diane 
Published: November 2017

Abstract
Using discourse institutionalism this article traces how Asian think tanks have provided valuable input for informing and articulating alternative economic narratives in the wake of the 1997 Asian Financial Crisis (AFC) and the Global Financial Crisis (GFC) of 2007–2008. Starting with the AFC, think tanks began to cultivate a move away from dominant western economic policy approaches towards more ‘Asian’-style solutions. These solutions included new policy programmes focused on creating ‘sustainable and balanced’ economic growth and protecting vulnerable populations from the negative impacts of financial downturns. Regulatory and institutional changes reflecting these new narratives helped shelter the Asian financial sector during the GFC a decade later. The GFC also created additional critical junctures permitting think tanks to coordinate new policy solutions concerning financial regulation and foster an emerging regional economic identity.
Tangent Cones of Lipschitz Normally Embedded Sets Are Lipschitz Normally Embedded. Appendix by Anne Pichon and Walter D. Neumann
Authors: Fernandes Alexandre , Sampaio J Edson 
Published: November 2017

AbstractWe prove that tangent cones of Lipschitz normally embedded sets are Lipschitz normally embedded. We also extend to real subanalytic sets the notion of reduced tangent cone and we show that subanalytic Lipschitz normally embedded sets have reduced tangent cones. In particular, we get that Lipschitz normally embedded complex analytic sets have reduced tangent cones.
Increasing the Power of Your Study by Increasing the Effect Size
Authors: Meyvis Tom , Van Osselaer Stijn M J 
Published: November 2017

Abstract
As in other social sciences, published findings in consumer research tend to overestimate the size of the effect being investigated, due to both file drawer effects and abuse of researcher degrees of freedom, including opportunistic analysis decisions. Given that most effect sizes are substantially smaller than would be apparent from published research, there has been a widespread call to increase power by increasing sample size. We propose that, aside from increasing sample size, researchers can also increase power by boosting the effect size. If done correctly, removing participants, using covariates, and optimizing experimental designs, stimuli, and measures can boost effect size without inflating researcher degrees of freedom. In fact, careful planning of studies and analyses to maximize effect size is essential to be able to study many psychologically interesting phenomena when massive sample sizes are not feasible.
How the Intention to Share Can Undermine Enjoyment: Photo-Taking Goals and Evaluation of Experiences
Authors: Barasch Alixandra , Zauberman Gal , Diehl Kristin 
Published: November 2017

AbstractPeople often share their experiences with others who were not originally present, which provides them with both personal and interpersonal benefits. However, most prior work on this form of sharing has examined the decision to share one’s experience only after the experience is over. We investigate a distinct, unexplored aspect of the sharing process: when the decision to share is already salient during an experience and hence can impact the experience itself. We examine this research question within the context of photo-taking, an increasingly ubiquitous and integral part of people’s experiences. Across two field and three laboratory studies, we find that relative to taking pictures for oneself (e.g., to preserve one’s memories), taking pictures with the intention to share them with others (e.g., to post on social media) reduces enjoyment of experiences. This effect occurs because taking photos with the intention to share increases self-presentational concern during the experience, which can reduce enjoyment directly, as well as indirectly by lowering engagement with the experience. We identify several factors that moderate the effect of photo-taking goals on enjoyment, such as individual differences in the extent to which individuals care about how others perceive them and the closeness of the intended audience.
An explanation of the formation of the peculiar periphery of Tycho's supernova remnant
Authors: Fang Jun , Yu Huan , Zhang Li 
Published: November 2017

Abstract
Tycho's supernova remnant (SNR) has a periphery that clearly deviates from a spherical shape, based on X-ray and radio observations. The forward shock from the south-east to the north of the remnant has a deformed outline with a depression in the east, although in the west it is generally round and smooth. Moreover, at some locations in the shell, the supernova ejecta is located close to the forward shock, resulting in protrusions. Using 3D hydrodynamical simulations, we studied the dynamical evolution of the supernova ejecta in an inhomogeneous medium and the formation process of the profile of the forward shock. In order to reproduce the peculiar periphery of the remnant, we propose a model in which the supernova ejecta has evolved in a cavity blown by a latitude-dependent outflow. The results indicate that the depression to the east and the protrusion to the south-east on the observed periphery of the remnant can be generally reproduced if we assume a wind bubble driven by an anisotropic wind with a mass-loss rate of ∼10−7 M⊙ yr−1, a pole velocity of ∼100 km s−1, a duration of ∼105 yr prior to the supernova explosion, and a spatial velocity of ∼30 km s−1 of the progenitor with respect to the circumstellar medium. In conclusion, an explanation of the peculiar shape of the periphery of Tycho's SNR is that the supernova ejecta evolved in the cavity driven by a latitude-dependent wind.
Spiral arms in thermally stratified protoplanetary discs
Authors: Juhász Attila , Rosotti Giovanni P 
Published: November 2017

Abstract
Spiral arms have been observed in nearly a dozen protoplanetary discs in near-infrared scattered light and recently also in the submillimetre continuum. While one of the most compelling explanations is that they are driven by planetary or stellar companions, in all but one cases such companions have not yet been detected and there is even ambiguity on whether the planet should be located inside or outside the spirals. Here, we use 3D hydrodynamic simulations to study the morphology of spiral density waves launched by embedded planets taking into account the vertical temperature gradient, a natural consequence of stellar irradiation. Our simulations show that the pitch angle of the spirals in thermally stratified discs is the lowest in the disc mid-plane and increases towards the disc surface. We combine the hydrodynamic simulations with 3D radiative transfer calculations to predict that the pitch angle of planetary spirals observed in the near-infrared is higher than in the submillimetre. We also find that in both cases the spirals converge towards the planet. This provides a new powerful observational method to determine if the perturbing planet is inside or outside the spirals, as well as map the thermal stratification of the disc.