bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purification of replicating pancreatic β-cells for gene expression studies
Authors: Carballar Reyes , Canyelles Maria de Lluc , Fernández Claudia , Martí Yasmina , Bonnin Sarah , Castaño Esther , Montanya Eduard , Téllez Noèlia 
Published: December 2017

Abstractβ-cell proliferation is a rare event in adult pancreatic islets. To study the replication-related β-cell biology we designed a replicating β-cells sorting system for gene expression experiments. Replicating β-cells were identified by EdU incorporation and purified by flow cytometry. For β-cell separation islet cells were sorted by size, granularity and Newport Green fluorescence emission that was combined with emitted fluorescence for EdU-labelled replicating cells sorting. The purity of the resulting sorted populations was evaluated by insulin staining and EdU for β-cell identification and for replicating cells, respectively. Total RNA was isolated from purified cell-sorted populations for gene expression analysis. Cell sorting of dispersed islet cells resulted in 96.2% purity for insulin positivity in the collected β-cell fraction and 100% efficiency of the EdU-based cell separation. RNA integrity was similar between FACS-sorted replicating and quiescent β-cells. Global transcriptome analysis of replicatingvsquiescent β-cells showed the expected enrichment of categories related to cell division and DNA replication. Indeed, key genes in the spindle check-point were the most upregulated genes in replicating β-cells. This work provides a method that allows for the isolation of replicating β-cells, a very scarce population in adult pancreatic islets.
Multibit memory operation of metal-oxide bi-layer memristors
Authors: Stathopoulos Spyros , Khiat Ali , Trapatseli Maria , Cortese Simone , Serb Alexantrou , Valov Ilia , Prodromakis Themis 
Published: December 2017

AbstractEmerging nanoionic memristive devices are considered as the memory technology of the future and have been winning a great deal of attention due to their ability to perform fast and at the expense of low-power and -space requirements. Their full potential is envisioned that can be fulfilled through their capacity to store multiple memory states per cell, which however has been constrained so far by issues affecting the long-term stability of independent states. Here, we introduce and evaluate a multitude of metal-oxide bi-layers and demonstrate the benefits from increased memory stability via multibit memory operation. We propose a programming methodology that allows for operating metal-oxide memristive devices as multibit memory elements with highly packed yet clearly discernible memory states. These states were found to correlate with the transport properties of the introduced barrier layers. We are demonstrating memory cells with up to 6.5 bits of information storage as well as excellent retention and power consumption performance. This paves the way for neuromorphic and non-volatile memory applications.
Substrate stiffness influences phenotype and function of human antigen-presenting dendritic cells
Authors: Mennens Svenja F. B. , Bolomini-Vittori Matteo , Weiden Jorieke , Joosten Ben , Cambi Alessandra , van den Dries Koen 
Published: December 2017

AbstractDendritic cells (DCs) are specialized immune cells that scan peripheral tissues for foreign material or aberrant cells and, upon recognition of such danger signals, travel to lymph nodes to activate T cells and evoke an immune response. For this, DCs travel large distances through the body, encountering a variety of microenvironments with different mechanical properties such as tissue stiffness. While immune-related pathological conditions such as fibrosis or cancer are associated with tissue stiffening, the role of tissue stiffness in regulating key functions of DCs has not been studied yet. Here, we investigated the effect of substrate stiffness on the phenotype and function of DCs by conditioning DCs on polyacrylamide substrates of 2, 12 and 50 kPa. Interestingly, we found that C-type lectin expression on immature DCs (iDCs) is regulated by substrate stiffness, resulting in differential antigen internalization. Furthermore, we show that substrate stiffness affects β2integrin expression and podosome formation by iDCs. Finally, we demonstrate that substrate stiffness influences CD83 and CCR7 expression on mature DCs, the latter leading to altered chemokine-directed migration. Together, our results indicate that DC phenotype and function are affected by substrate stiffness, suggesting that tissue stiffness is an important determinant for modulating immune responses.
Plasma acylcarnitine concentrations reflect the acylcarnitine profile in cardiac tissues
Authors: Makrecka-Kuka Marina , Sevostjanovs Eduards , Vilks Karlis , Volska Kristine , Antone Unigunde , Kuka Janis , Makarova Elina , Pugovics Osvalds , Dambrova Maija , Liepinsh Edgars 
Published: December 2017

AbstractIncreased plasma concentrations of acylcarnitines (ACs) are suggested as a marker of metabolism disorders. The aim of the present study was to clarify which tissues are responsible for changes in the AC pool in plasma. The concentrations of medium- and long-chain ACs were changing during the fed-fast cycle in rat heart, muscles and liver. After 60 min running exercise, AC content was increased in fasted mice muscles, but not in plasma or heart. After glucose bolus administration in fasted rats, the AC concentrations in plasma decreased after 30 min but then began to increase, while in the muscles and liver, the contents of medium- and long-chain ACs were unchanged or even increased. Only the heart showed a decrease in medium- and long-chain AC contents that was similar to that observed in plasma. In isolated rat heart, but not isolated-contracting mice muscles, the significant efflux of medium- and long-chain ACs was observed. The efflux was reduced by 40% after the addition of glucose and insulin to the perfusion solution. Overall, these results indicate that during fed-fast cycle shifting the heart determines the medium- and long-chain AC profile in plasma, due to a rapid response to the availability of circulating energy substrates.
Circulating bone morphogenetic protein-9 in relation to metabolic syndrome and insulin resistance
Authors: Xu Xiaohui , Li Xiaoqiang , Yang Gangyi , Li Ling , Hu Wenjing , Zhang Lili , Liu Hua , Zheng Hongting , Tan Minghong , Zhu Danping 
Published: December 2017

AbstractOur objective is to determine circulating Bone morphogenetic protein-9(BMP-9) levels in subjects with Metabolic Syndrome (MetS) and examine the relationship between BMP-9 and conventional markers for MetS and insulin resistance (IR). A total of 362 newly diagnosed patients with MetS along with healthy controls were recruited for this cross-sectional study. Circulating BMP-9 levels were measured by ELISA. Circulating BMP-9 levels were significantly lower in MetS patients compared to those of the healthy controls. BMP-9 was associated negatively with Waist hip ratio (WHR), fasting blood glucose (FBG), 2-hour blood glucose after glucose overload (2h-OGTT), HbA1c, triglyceride (TG) levels and HOMA-IR and positively with free fatty acid (FFA) and HDL after control for age and sex. In a multiple linear regression, BMP-9 was independently associated with type 2 diabetes mellitus (T2DM), HOMA-IR and FFA. Binary logistic regression showed that plasma BMP-9 concentrations were significantly associated with MetS even after controlling for anthropometric variables and lipid profiles. In addition, circulating BMP-9 levels reduced progressively with an increasing number of MetS components. The best cutoff values for circulating BMP-9 to predict MetS was 56.6 ng/L. Circulating BMP-9 levels were associated with the key components of MetS and IR.
Ubiquitin-conjugating enzyme E2T (UBE2T) and denticleless protein homolog (DTL) are linked to poor outcome in breast and lung cancers
Authors: Perez-Peña Javier , Corrales-Sánchez Verónica , Amir Eitan , Pandiella Atanasio , Ocana Alberto 
Published: December 2017

AbstractProtein ubiquitination and degradation represent druggable vulnerabilities of cancer cells. We used gene expression and functional annotation analyses to identify genes in the ubiquitin pathway which are differentially expressed between normal breast and basal-like tumors. With this approach we identified 16 ubiquitin related genes overexpressed in basal-like breast cancers compared with normal breast. We then explored the association between these genes and outcomes using the KMPlotter online tool. Two genes, the ubiquitin-conjugating enzyme E2T (UBE2T) and the denticleless protein homolog (DTL) were overexpressed and linked with detrimental outcome in basal-like and luminal breast cancer patients. Furthermore, we found that UBE2T and DTL were amplified in around 12% of breast tumors based on data contained at cBioportal. In non-small cell lung adenocarcinomas, UBE2T and DTL were also amplified in around 7% of cases and linked with disease recurrence after surgical resection. No significant molecular alterations or a clear trend for clinical outcome was observed for these genes in ovarian serous cystadenocarcinoma, esophagus-stomach cancer or non-small squamous cell carcinoma. Our data suggest that UBE2T and DTL may have a role in the pathophysiology of breast and lung tumors, opening avenues for future clinical evaluation of agents targeting those proteins or their pathways.
Optical sparse aperture imaging with faint objects using improved spatial modulation diversity
Authors: Xie Zongliang , Ma Haotong , Qi Bo , Ren Ge , He Xiaojun , Dong Li , Tan Yufeng , Qiao Shan 
Published: December 2017

AbstractThe next generation of optical sparse aperture systems will provide high angular resolution for astronomical observations. Spatial modulation diversity (SMD) is a newly developed post-processing technique for such telescopes, faced with challenges of imaging faint objects, which are very attractive for astronomers but always make raw diversity images suffer serious photon noise. In this paper, we propose an improved SMD with denoising reprocessing embedded to address the problem. The blocking-matching and 3D filtering algorithm, a state-of-the-art denoising technique, is first employed to process the diversity images with low photon intensities generated by spatial modulation, specifically switching off each sub-aperture sequentially. SMD algorithm then can be applied to estimate wavefront and digitally restore images. It is demonstrated by both simulations and experiments that the proposed method outperforms the previous SMD in terms of reconstructions of wavefront and imagery from the raw images of faint objects corrupted seriously by photon noise. The reported method may provide an alternative approach to acquire high-quality images of faint objects for astronomical observations of the future segmented mirrors or telescope arrays.
Selectable phase formation in VAlN thin films by controlling Al+ subplantation depth
Authors: Greczynski G. , Mráz S. , Hultman L. , Schneider J. M. 
Published: December 2017

AbstractWe report on a thin film synthesis technique which allows for unprecedented control over the crystalline phase formation in metastable transition metal nitride based layers. For the model material system of V0.26Al0.74N, a complete transition from hexagonal to supersaturated cubic structure is achieved by tuning the incident energy, hence subplantation depth, of Al+ metal ions during reactive hybrid high power impulse magnetron sputtering of Al target and direct current magnetron sputtering of V target in Ar/N2 gas mixture. These findings enable the phase selective synthesis of novel metastable materials that combine excellent mechanical properties, thermal stability, and oxidation resistance.
Bone mineral density measurements in vertebral specimens and phantoms using dual-layer spectral computed tomography
Authors: Mei Kai , Schwaiger Benedikt J. , Kopp Felix K. , Ehn Sebastian , Gersing Alexandra S. , Kirschke Jan S. , Muenzel Daniela , Fingerle Alexander A. , Rummeny Ernst J. , Pfeiffer Franz , Baum Thomas , Noël Peter B. 
Published: December 2017

AbstractTo assess whether phantomless calcium-hydroxyapatite (HA) specific bone mineral density (BMD) measurements with dual-layer spectral computed tomography are accurate in phantoms and vertebral specimens. Ex-vivo human vertebrae (n = 13) and a phantom containing different known HA concentrations were placed in a semi-anthropomorphic abdomen phantom with different extension rings simulating different degrees of obesity. Phantomless dual-layer spectral CT was performed at different tube current settings (500, 250, 125 and 50 mAs). HA-specific BMD was derived from spectral-based virtual monoenergetic images at 50 keV and 200 keV. Values were compared to the HA concentrations of the phantoms and conventional qCT measurements using a reference phantom, respectively. Above 125 mAs, errors for phantom measurements ranged between −1.3% to 4.8%, based on spectral information. In vertebral specimens, high correlations were found between BMD values assessed with spectral CT and conventional qCT (r ranging between 0.96 and 0.99; p < 0.001 for all) with different extension rings, and a high agreement was found in Bland Altman plots. Different degrees of obesity did not have a significant influence on measurements (P > 0.05 for all). These results suggest a high validity of HA-specific BMD measurements based on dual-layer spectral CT examinations in setups simulating different degrees of obesity without the need for a reference phantom, thus demonstrating their feasibility in clinical routine.
Genetically unmatched human iPSC and ESC exhibit equivalent gene expression and neuronal differentiation potential
Authors: Marei Hany E. , Althani A. , Lashen S. , Cenciarelli C. , Hasan Anwarul 
Published: December 2017

AbstractThe potential uniformity between differentiation and therapeutic potential of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) remains debatable. We studied the gene expression profiles, pathways analysis and the ability to differentiated into neural progenitor cells (NPCs) and motor neurons (MNs) of genetically unmatched integration-free hiPSC versus hESC to highlight possible differences/similarities between them at the molecular level. We also provided the functional information of the neurons derived from the different hESCs and hiPSCs lines using the Neural Muscular Junction (NMJ) Assay. The hiPSC line was generated by transfecting human epidermal fibroblasts (HEF) with episomal DNAs expressing Oct4, Sox2, Klf4, Nanog, L-Myc and shRNA against p53. For the hESCs line, we used the NIH-approved H9 cell line. Using unsupervised clustering both hESCs and hiPSCs were clustered together implying homogeneous genetic states. The genetic profiles of hiPSCs and hESCs were clearly similar but not identical. Collectively, our data indicate close molecular similarities between genetically unmatched hESCs and hiPS in term of gene expression, and signaling pathways. Moreover, both cell types exhibited similar cholinergic motor neurons differentiation potential with marked ability of the differentiated hESCs and hiPSCs-derived MNs to induce contraction of myotubes after 4 days of co-culture.
Identification of Newly Committed Pancreatic Cells in the Adult Mouse Pancreas
Authors: Socorro Mairobys , Criscimanna Angela , Riva Patricia , Tandon Manuj , Prasadan Krishna , Guo Ping , Humar Abhinav , Husain Sohail Z. , Leach Steven D. , Gittes George K. , Esni Farzad 
Published: December 2017

AbstractMultipotent epithelial cells with high Aldehyde dehydrogenase activity have been previously reported to exist in the adult pancreas. However, whether they represent true progenitor cells remains controversial. In this study, we isolated and characterized cells with ALDH activity in the adult mouse or human pancreas during physiological conditions or injury. We found that cells with ALDH activity are abundant in the mouse pancreas during early postnatal growth, pregnancy, and in mouse models of pancreatitis and type 1 diabetes (T1D). Importantly, a similar population of cells is found abundantly in healthy children, or in patients with pancreatitis or T1D. We further demonstrate that cells with ALDH activity can commit to either endocrine or acinar lineages, and can be divided into four sub-populations based on CD90 and Ecadherin expression. Finally, our in vitro and in vivo studies show that the progeny of ALDH1+/CD90−/Ecad− cells residing in the adult mouse pancreas have the ability to initiate Pancreatic and duodenal homeobox (Pdx1) expression for the first time. In summary, we provide evidence for the existence of a sortable population of multipotent non-epithelial cells in the adult pancreas that can commit to the pancreatic lineage following proliferation and mesenchymal to epithelial transition (MET).
The Potential of Past Tense Marking in Oral Reading as a Clinical Marker of Specific Language Impairment in School-Age Children
Authors: Werfel Krystal L. , Hendricks Alison Eisel , Schuele C. Melanie 
Published: December 2017

Purpose
The purpose of this study was twofold. The first aim was to explore differences in profiles of past tense marking in oral reading of school-age children with specific language impairment (SLI). The second aim was to explore the potential of past tense marking in oral reading as a clinical marker of SLI in school-age children.

Method
This study examined oral readings of connected text to describe the frequency and type of reading errors on regular and irregular past tense verbs for 21 children with SLI as compared to 30 children with typical language in Grades 2 and 3. Each past tense verb token was categorized into 1 of 6 mutually exclusive response types: (a) correctly marked past tense, (b) overmarked past tense, (c) bare stem, (d) other verb inflection, (e) nonverb, or (f) no response. Performance across groups was compared. Additionally, classification statistics were calculated at several cutoffs for regular past tense accuracy and regular past tense finiteness marking.

Results
For regular past tense, there was a significant group difference on accuracy. Children with SLI were less accurate at marking past tense when in oral reading than typical language peers; other response types did not differ. For irregular past tense, there were no group differences. In addition, there was a significant group difference on finiteness marking; this difference was driven by regular but not irregular verbs. A cutoff of 90% for regular past tense accuracy yielded moderate sensitivity and specificity; no cutoff for regular past tense finiteness marking yielded sensitivity above 70%.

Conclusions
Regular past tense accuracy in oral reading provides promise as a clinical marker for diagnosing SLI in school-age children.
The Effects of Palate Features and Glossectomy Surgery on /s/ Production
Authors: Grimm Dana L. , Stone Maureen , Woo Jonghye , Lee Junghoon , Hwang Jun-Hyuk , Bedrosian Gary E. , Prince Jerry L. 
Published: December 2017

Purpose
The aims of this article were to determine the effects of hard palate morphology and glossectomy surgery on tongue position and shape during /s/ for patients with small tumors. The first expectation was that laminal /s/ would be more prevalent in patients, than apical, due to reduced tongue tip control after surgery. The second was that patients would hold the tongue more anteriorly than controls to compensate for reduced tongue mass.

Method
Three-dimensional tongue volumes were calculated from magnetic resonance imaging for the whole tongue and the portion anterior to the first molar during the /s/ in /əsuk/ for 21 controls and 14 patients. These volumes were used to calculate tongue anteriority and cross-sectional shape. Dental casts were used to measure palate perimeter, height, and width of the hard palate.

Results

Palate height correlated with tongue height in controls (
p
< .05), but not patients. In patients, tongue anteriority correlated negatively with canine width and cross-sectional tongue shape (
p
< .05). Controls with a high palate favored laminal /s/. Patients preferred laminal /s/ regardless of palate height (
p
< .01).

Conclusions
For controls, hard palate height affected tongue height; a higher palate yielded a higher tongue. For patients, hard palate width affected tongue width; a narrower palate yielded a more anterior tongue. Tongue shape was unaffected by any palate features. Preference for /s/ showed an interaction effect between subject and palate height. Controls with high palates preferred a laminal /s/. All patients preferred a laminal /s/; glossectomy surgery may reduce tongue tip control.
Distinguishing the albedo of exoplanets from stellar activity
Authors: Serrano L. M. , Barros S. C. C. , Oshagh M. , Santos N. C. , Faria J. P. , Demangeon O. , Sousa S. G. , Lendl M. 
Published: December 2017

Context. Light curves show the flux variation from the target star and its orbiting planets as a function of time. In addition to the transit features created by the planets, the flux also includes the reflected light component of each planet, which depends on the planetary albedo. This signal is typically referred to as phase curve and could be easily identified if there were no additional noise. As well as instrumental noise, stellar activity, such as spots, can create a modulation in the data, which may be very difficult to distinguish from the planetary signal.
Aims. We analyze the limitations imposed by the stellar activity on the detection of the planetary albedo, considering the limitations imposed by the predicted level of instrumental noise and the short duration of the obervations planned in the context of the CHEOPS mission.
Methods. As initial condition, we have assumed that each star is characterized by just one orbiting planet. We built mock light curves that included a realistic stellar activity pattern, the reflected light component of the planet and an instrumental noise level, which we have chosen to be at the same level as predicted for CHEOPS. We then fit these light curves to try to recover the reflected light component, assuming the activity patterns can be modeled with a Gaussian process.
Results. We estimate that at least one full stellar rotation is necessary to obtain a reliable detection of the planetary albedo. This result is independent of the level of noise, but it depends on the limitation of the Gaussian process to describe the stellar activity when the light curve time-span is shorter than the stellar rotation. As an additional result, we found that with a 6.5 magnitude star and the noise level of CHEOPS, it is possible to detect the planetary albedo up to a lower limit of Rp = 0.03 R*. Finally, in presence of typical CHEOPS gaps in the simulations, we confirm that it is still possible to obtain a reliable albedo.