bluuebunny/crossref_metadata_2025_split huggingface dataset:
CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis
Authors: Bondue Tjessa , Cervellini Francesca , Smeets Bart , Strelkov Sergei V. , Horuz‐Engels Flore , Veys Koenraad , Vargas‐Poussou Rosa , Matteis Maria Antonietta De , Staiano Leopoldo , van den Heuvel Lambertus , Levtchenko Elena 
Published: September 2024

AbstractRenal proximal tubular reabsorption of proteins and polypeptides is tightly regulated by a concerted action of the multi‐ligand receptors with subsequent processing from the clathrin‐coated pits to early/recycling and late endosomes and towards lysosomes. We performed whole exome‐sequencing in a male patient from a consanguineous family, who presented with low‐ and intermediate molecular weight proteinuria, nephrocalcinosis and oligospermia. We identified a new potential player in tubular endocytosis, coiled‐coil domain containing 158 (CCDC158). The variant in CCDC158 segregated with the phenotype and was also detected in a female sibling with a similar clinical kidney phenotype. We demonstrated the expression of this protein in kidney tubules and modeled its structure in silico. We hypothesized that the protein played a role in the tubular endocytosis by interacting with other endocytosis regulators, and used mass spectrometry to identify potential interactors. The role of CCDC158 in receptor‐mediated endocytosis was further confirmed by transferrin and GST‐RAP trafficking analyses in patient‐derived proximal tubular epithelial cells. Finally, as CCDC158 is known to be expressed in the testis, the presence of oligospermia in the male sibling further substantiated the pathogenic role of the detected missense variant in the observed phenotype. In this study, we provide data that demonstrate the potential role of CCDC158 in receptor‐mediated endocytosis, most likely by interaction with other endocytosis‐related proteins that strongly correlate with the proximal tubular dysfunction phenotype as observed in the patients. However, more studies are needed to fully unravel the molecular mechanism(s) in which CCDC158 is involved.
Detection of Bartonella henselae in feline erythrocytes in Egypt by using Giemsa staining, transmission electron microscopy, and polymerase chain reaction
Authors: Alsaadawy Reem M. , Sayed Amal S. M. , Ali Magda M. , Abd‐Elghaffar Sary Kh. 
Published: September 2024

AbstractBartonella species (Bartonella spp.) have gained recognition as a significant human pathogen, implicated in a wide range of diseases. Among these, Bartonella henselae infection has been extensively studied for its primary occurrence in cats and its role in the development of cat‐scratch disease in humans. While light microscopy and transmission electron microscopy (TEM) have traditionally played crucial roles in identifying causative agents of infectious diseases, including Bartonella spp., the accuracy of these methods in identifying Bartonella spp. remains undefined. Therefore, this study aims to bridge this gap by employing both light microscopy and TEM to detect Bartonella in feline blood samples and to confirm B. henselae with polymerase chain reaction (PCR). Examination of blood smears stained with Giemsa and toluidine blue semithin sections by using light microscopy revealed the presence of intraerythrocytic corpuscles, suggesting Bartonella infection in six out of 33 examined cat blood samples. TEM findings corroborated these observations, showcasing the engulfment of bacteria by the erythrocyte membrane, along with the presence of some Bartonella spp., adhering to the erythrocyte wall. PCR‐based molecular detection confirmed the presence of B. henselae in these six samples. It is concluded that light microscopy and TEM are considered valuable in the screening of cats' blood for the potential presence of Bartonella. However, further molecular techniques are essential for precise identification and confirmation of specific Bartonella spp.Research Highlights
Giemsa‐stained blood smear and semithin section showed potential intraerythrocytic Bartonella spp. corpuscles.
TEM demonstrated the engulfment of Bartonella spp. by the erythrocyte membrane, along with the presence of some Bartonella spp. adhering to the erythrocyte wall.
Molecular analysis of blood samples from cats by PCR unveiled that six out of 33 (18.18%) samples tested positive for B. henselae infection.
Biofabrication of GO–Ag nanocomposite using Cucumis callosus (kachri) fruits: Enhanced antibacterial properties and green synthesis approach
Authors: Mamata  , Kumar Chandra , Tiwari Vishvanath , Ţălu Ştefan , Awasthi Kamlendra , Dutta Anirban 
Published: September 2024

AbstractThis study presents a novel, environmentally sustainable method for the synthesis of graphene oxide (GO) sheets decorated uniformly with silver nanoparticles (Ag NPs) ranging in size from 4 to 34 nm. The reduction of AgNO3 is achieved using an extract derived from Cucumis callosus fruit, which serves as a dual‐function stabilizing and reducing agent. Cucumis callosus, belonging to the Cucurbitaceae family and native to regions such as India, South America, Thailand, Africa, and Egypt, is recognized for its substantial nutritional and medicinal value, encompassing antioxidant, antidiabetic, anticancer, and anti‐inflammatory properties. In this study, we explore the utilization of Cucumis callosus extract for the first time in synthesizing Ag NPs, employing a green synthesis approach to produce GO–Ag nanocomposites. Comprehensive characterization techniques confirm the structural integrity and quality of the synthesized nanocomposites. The antibacterial efficacy of the green‐synthesized Ag‐decorated GO nanocomposites was evaluated using the disk diffusion method against Bacillus subtilis (Gram‐positive) and Escherichia coli (Gram‐negative) bacteria at varying dosages. The nanocomposites demonstrated dose‐dependent antibacterial activity against both bacterial strains, with a notably heightened effect observed against Gram‐negative bacteria. These findings underscore the potential of Cucumis callosus as a promising candidate for the sustainable preparation of GO–Ag nanocomposites with enhanced antibacterial properties, suitable for various biomedical and environmental applications.Research Highlights
This work presents a simple, environmentally free, and cost‐effective green synthesis method to decorate uniformly small (4–34 nm) spherical Ag NPs on the GO sheets.
Ag NPs were produced by reducing AgNO3 using Cucumis callosus fruit extract as a stabilizing and reducing agent.
The nanocomposites show dosage‐dependent antibacterial activities against both Gram‐positive and Gram‐negative bacteria, but the antibacterial effect is higher against the Gram‐negative bacteria.
Synthesis of these nanocomposites via the green route using an herbal plant/fruit like Cucumis callosus will benefit the medical industry.
Benchmarking transcriptome deconvolution methods for estimating tissue‐ and cell‐type‐specific extracellular vesicle abundances
Authors: Larsen Jannik Hjortshøj , Jensen Iben Skov , Svenningsen Per 
Published: September 2024

AbstractExtracellular vesicles (EVs) contain cell‐derived lipids, proteins and RNAs; however, determining the tissue‐ and cell‐type‐specific EV abundances in body fluids remains a significant hurdle for our understanding of EV biology. While tissue‐ and cell‐type‐specific EV abundances can be estimated by matching the EV's transcriptome to a tissue's/cell type's expression signature using deconvolutional methods, a comparative assessment of deconvolution methods' performance on EV transcriptome data is currently lacking. We benchmarked 11 deconvolution methods using data from four cell lines and their EVs, in silico mixtures, 118 human plasma and 88 urine EVs. We identified deconvolution methods that estimated cell type‐specific abundances of pure and in silico mixed cell line‐derived EV samples with high accuracy. Using data from two urine EV cohorts with different EV isolation procedures, four deconvolution methods produced highly similar results. The three methods were also concordant in their tissue‐ and cell‐type‐specific plasma EV abundance estimates. We identified driving factors for deconvolution accuracy and highlighted the importance of implementing biological knowledge in creating the tissue/cell type signature. Overall, our analyses demonstrate that the deconvolution algorithms DWLS and CIBERSORTx produce highly similar and accurate estimates of tissue‐ and cell‐type‐specific EV abundances in biological fluids.
Early Oxidative Stress May Prevent a Red Ornament From Signaling Longevity
Authors: Romero‐Haro A. A. , Cantarero A. , Alonso‐Alvarez C. 
Published: September 2024

ABSTRACTHarsh early environmental conditions can exert delayed, long‐lasting effects on phenotypes, including reproductive traits such as sexual signals. Indeed, adverse early conditions can accelerate development, increasing oxidative stress that may, in turn, impact adult sexual signals. Among signals, colorations produced by red ketocarotenoids seem to depend on mitochondrial functioning. Hence, they could reveal individual cell respiration efficiency. It has been hypothesized that these traits are unfalsifiable “index” signals of condition due to their deep connection to individual metabolism. Since mitochondrial dysfunction is frequently linked to aging, red ketocarotenoid‐based ornaments could also be good signals of a critical fitness component: longevity. We tested this red color per longevity correlation in captive zebra finches. In addition, we experimentally decreased the synthesis of glutathione (a critical intracellular antioxidant) during the first days of the birds' life to resemble harsh early environmental conditions (e.g., undernutrition). Longevity was recorded until the death of the last bird (almost 9 years). Males, but not females, exhibiting a redder bill in early adulthood lived longer than males with paler bills, which agrees with some precedent studies. However, such bill redness—longevity connection was absent among males with inhibited glutathione synthesis. These findings may suggest that environmental factors can alter the reliability of red ketocarotenoid‐based sexual signals, making them less unfalsifiable than believed.
Protein‐added kefir: biochemical changes in in vitro digestion stages
Authors: Saygili Derya , Karagozlu Cem 
Published: September 2024

AbstractBACKGROUNDWhile yogurt is the leading fermented milk product, kefir is at the top of the beverage scale. Milk proteins, on the other hand, show specific functions that positively affect healthy nutrition due to the bioactive components, that they provide the necessary amino acids for growth and development.RESULTSIn our study, kefir, a functional product enriched with whey proteins, casein and skimmed milk powder, which are the natural components of milk, was produced. Added‐protein kefir samples were applied the in vitro digestion protocol, static method. In order to observe different protein behaviors, samples were taken pre‐digestion, at 120th minute and at 240th minute of digestion protocol. ACE and Antioxidant capacity determination analyzes were carried out. While ACE inhibition values were in the range of 78.63–90.30% pre‐digestion, they changed in the range of 86.97–96.38% after gastrointestinal digestion. It was determined that the ACE inhibition values of the control sample remained at the lowest level at all stages of digestion and that the difference between all of samples was significant (P < 0.05). Antioxidant activity value ranging from 0.3615–0.5512 meq Ascorbic acid/μg before digestion was determined as 1.3796–1.9313 meq Ascorbic acid/μg after gastrointestinal digestion (P < 0.05).CONCLUSIONKefir samples containing whey protein stand out with their high potential in terms of both antioxidant activity capacity and ACE inhibition activity at all stages of digestion. Considering their therapeutic effects in fermented products, it is thought that whey proteins among milk proteins will be important alternative sources to enrich the protein content in kefir production. © 2024 Society of Chemical Industry.
Characterization of a Soft Ionization by Chemical Reaction in Transfer Ion Source Hyphenated With Supercritical Fluid Chromatography‐Tandem Mass Spectrometry
Authors: Bhakta Niray , Kaplitz Alexander S. , Black Destini , Schug Kevin A. 
Published: September 2024

ABSTRACTA commercially available dielectric barrier discharge ionization (DBDI) source was tested with supercritical fluid chromatography‐mass spectrometry (SFC‐MS). The compound mixture investigated comprised caffeine, theobromine, theophylline, uracil, testosterone, and pyrene, diluted in methanol. Dynamic response ranges were evaluated with multiple injections at different concentrations. Precision studies demonstrated the robustness and sensitivity of the ionization source across a concentration range of 10–1000 ng/mL. Results from this experiment showed linear regression of 0.99 or greater for all analytes tested over the range with a relative standard deviation (RSD) of less than 10% down to 10 ng/mL for all analytes except theobromine, which had an RSD of less than 10% down to 25 ng/mL. Notably, this study marks the first investigation of sensitivity for coupling a commercial DBDI source with SFC; a limit of detection less than 1 ng/mL was achieved for all compounds. This study demonstrates chromatographic separation by SFC and MS analysis for compounds that ionize poorly using traditional atmospheric pressure ionization, such as polycyclic aromatic hydrocarbons. Combining SFC with the DBDI source opens promising avenues for analyzing compounds that were previously challenging to characterize with standard atmospheric pressure ionization techniques.
Responding to Hitch in Fighting Mycobacterium Tuberculosis Through Arginine Multi Functionalized Mucoadhesive SNEDDS of Rifampicin
Authors: Saeed Sana , Farooq Muhammad , Arshad Rabia , Adnan Sherjeel , Ahmad Hammad , Masood Zeeshan , Malik Abdul , Saeed Ayesha , Tabish Tanveer A 
Published: September 2024

AbstractThe study aimed to develop thiolated pluronic‐based self‐emulsifying drug delivery system (SNEDDS) targeted delivery of Rifampicin coated by arginine for enhanced drug loading, mucoadhesion, muco penetration, site‐specific delivery, stabilized delivery against intracellular mycobacterium tuberculosis (M. tb), decreased bacterial burden and production by intracellular targeting. Oleic oil, PEG 200 and Tween 80 are selected as oil, co‐surfactant and surfactant based on solubilizing capacity and pseudo ternary diagram region. Coating of thiolated polymer on SNEDDS with ligand arginine (Arg‐Th‐F407 SNEDDDS) decreased bacterial burden and production by intracellular targeting in macrophages. Formulation are evaluated through scanning electron microscope (SEM), EDAX analysis, diffraction laser scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, and thermal analysis (DSC & TGA). Hydrodynamic diameter of thiolated polymeric SNEDDS (Th‐F407 SNEDDS) and Arg‐Th‐F407 SNEDDS is observed to be 148.4 and 188.5 nm with low PDI of 0.4 and 0.3, respectively. Invitro drug release study from Arg‐Th‐F407 SNEDDS indicates 80% sustained release in 72 h under controlled conditions. Arg‐Th‐F407 SNEDDDS shows excellent capability of killing M.tb strains in macrophages even at low dose as compared to traditional rifampicin (RIF) and is found biocompatible, non‐cytotoxic, and hemocompatible. Therefore, Arg‐Th‐F407 SNEDDDS of RIF proved ideal for targeting and treating M.tb strains within macrophages.
Effect of argon on classification and identification of organics based on laser‐induced breakdown spectroscopy
Authors: Li Yeqiu , Zhao Jingyi , Cai Mingyang , Duan Hao , Li Qian , Dai Qin , Wu Rina 
Published: September 2024

AbstractOrganic matter is the material basis of life, which is widely present in people's products and lives. The rapid and accurate detection of organic matter can be applied to the identification of fake and inferior products, the traceability of the origin of agricultural products, and the early warning of explosives in antiterrorism. By combining laser‐induced breakdown spectroscopy (LIBS) with argon, the spectral characteristics of five common organic compounds (nitroglycerin, metronidazole, polyformaldehyde, polypropylene, and phenolic resin), the plasma lifetime, the parameters of plasma thermodynamic state, and the distribution of three existing forms of the element C in two gas environments were compared, the influence of argon on organic LIBS results and the reasons for its enhancement were analyzed. The results indicate that argon makes the atomization of carbon chain structures in organic matter more complete, and reduce the interference of the element N in air on the organic LIBS results to a certain extent. The intensity differences of different organic substances are more obvious. support vector machine optimized by principal component analysis and particle swam optimization algorithm was used to classify five kinds of organic matter. The prediction accuracy of classification in air is 94.4%, and it improves to 100% in argon. This result provides a powerful method for high‐precision, real‐time in‐situ, and rapid identification of organic substances, which has important scientific significance for the study of organic substances LIBS.
‘We are working in specialty units’—An exploratory qualitative study
Authors: Rangwaneni Mphedziseni Esther , Raliphaswa Ndidzulafhi Selina , Maluleke Mary , Letlalo Vusiwana Patricia , Masutha Thingahangwi Cecilia , Manyuma Duppy , Makhado Langanani , Rikhotso Tinyiko Nelly 
Published: September 2024

AbstractAimTo explore related support needs of general nurses in specialty mental health units and provide references for formulating a model to support this population working in mental health care units.DesignAn exploratory qualitative design.MethodIn‐depth individual unstructured interviews were performed with 15 general nurses who worked in mental health care units and were selected through purposive sampling. Data were collected through in‐depth, individual, unstructured interviews. Data were analysed thematically using Tech's eight‐step analysis method.ResultsTwo themes with their subthemes were extracted. The two themes included (a) Needs for professional growth and (b) the Need for emotional support.ConclusionInfluenced by many factors, general nurses could not perform some nursing activities in mental health care units. Health managers should plan the training program to empower and provide emotional support to the general nurses in mental health care units. Further research is required to develop a model to facilitate the support of general nurses allocated to these units.Reporting MethodThis study follows the consolidated criteria for reporting qualitative research.Public ContributionA total of 15 general nurses participated in the study. We utilized their lunch time to conduct the interviews, significantly contributing to the article's content.
Impact of Ligand Design on an Iron NHC Epoxidation Catalyst
Authors: Schlachta Tim P. , Zámbó Greta G. , Sauer Michael J. , Rüter Isabelle , Kühn Fritz E. 
Published: September 2024

AbstractAn open‐chain iron pyridine‐NHC framework is expanded utilizing a benzimidazole moiety to deepen the understanding of the impact of electronic variations on iron NHC epoxidation catalysts, especially regarding the stability. The thereby newly obtained iron(II) NHC complex is characterized and employed in olefin epoxidation. It is remarkably temperature tolerant and achieves a TOF of ca. 10 000 h−1 and TON of ca. 700 at 60 °C in the presence of the Lewis acid Sc(OTf)3, displaying equal stability, but lower activity than the unmodified iron pyridine‐NHC (pre‐)catalyst. In addition, a synthetic approach towards another ligand containing 2‐imidazoline units is described but formylation as well as hydrolysis hamper its successful synthesis.
Development of a Proteomic Workflow for the Identification of Heparan Sulphate Proteoglycan‐Binding Substrates of ADAM17
Authors: Calligaris Matteo , Spanò Donatella Pia , Puccio Maria Chiara , Müller Stephan A. , Bonelli Simone , Lo Pinto Margot , Zito Giovanni , Blobel Carl P. , Lichtenthaler Stefan F. , Troeberg Linda , Scilabra Simone Dario 
Published: September 2024

ABSTRACTEctodomain shedding, which is the proteolytic release of transmembrane proteins from the cell surface, is crucial for cell‐to‐cell communication and other biological processes. The metalloproteinase ADAM17 mediates ectodomain shedding of over 50 transmembrane proteins ranging from cytokines and growth factors, such as TNF and EGFR ligands, to signalling receptors and adhesion molecules. Yet, the ADAM17 sheddome is only partly defined and biological functions of the protease have not been fully characterized. Some ADAM17 substrates (e.g., HB‐EGF) are known to bind to heparan sulphate proteoglycans (HSPG), and we hypothesised that such substrates would be under‐represented in traditional secretome analyses, due to their binding to cell surface or pericellular HSPGs. Thus, to identify novel HSPG‐binding ADAM17 substrates, we developed a proteomic workflow that involves addition of heparin to solubilize HSPG‐binding proteins from the cell layer, thereby allowing their mass spectrometry detection by heparin‐treated secretome (HEP‐SEC) analysis. Applying this methodology to murine embryonic fibroblasts stimulated with an ADAM17 activator enabled us to identify 47 transmembrane proteins that were shed in response to ADAM17 activation. This included known HSPG‐binding ADAM17 substrates (i.e., HB‐EGF, CX3CL1) and 14 novel HSPG‐binding putative ADAM17 substrates. Two of these, MHC‐I and IL1RL1, were validated as ADAM17 substrates by immunoblotting.
Sorption of non‐ferrous metal ions from aqueous solutions by polymer compositions based on polyacrylic acid and bentonite clay
Authors: Makhkamov Muzaffar , Mirzakulov Umid , Xidirova Mavluda , Abdugapporova Gulchekhrakhon 
Published: September 2024

AbstractComposite hydrogels (CH) were obtained by copolymerization of acrylic acid with N,N‐methylene‐bis‐acrylamide in the suspension of bentonite clay (BC) by the method “in situ.” At this CH of cross‐linking structure was formed in which, in the space between parts of polymer hydrogels (PH) on the base of polyacrylic acid (PAAc), were introduced particles of mineral. The morphology and structure of CH have been investigated by scanning electron microscopy, which has shown that particles of BC are distributed throughout the whole volume of the composite. The sorption of Cu2+, Ni2+, and Cr3+ ions from aqueous solutions of their salts by sorbents using the spectrophotometric method was studied. It has been determined that the introduction of BC particles into the composition of PH leads to a noticeable increase in their sorption capacity. Determination of the elemental analysis of the composition of sorbents after sorption by X‐ray fluorescence analysis showed that the sorption of non‐ferrous metal ions increases in the row Cr3+ < Ni2+ < Cu2+.