bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering of an H2O2 auto‐scavenging in vivo cascade for pinoresinol production
Authors: Lv Yongkun , Cheng Xiaozhong , Du Guocheng , Zhou Jingwen , Chen Jian 
Published: April 2017

ABSTRACTPinoresinol is a natural lignan with a high market value that has potential pharmacological and food supplement applications. Pinoresinol is currently isolated from plants, which suffers from low efficiency and yield. To produce pinoresinol from inexpensive and industrially available eugenol, an in vivo enzymatic cascade composed of vanillyl alcohol oxidase and peroxidase was designed, which scavenges H2O2 automatically and eliminates protein purification and cofactor addition. Two peroxidases were screened and identified from Escherichia coli BL21 (DE3), and tested in the enzymatic cascade. To balance the flux, different genetic architectures were constructed by using ePathBrick and fusion gene approaches. Scavenging H2O2 alleviated by‐product toxicity and enzyme inhibition, and led to efficient pinoresinol production. Optimization of the reaction conditions achieved a titer of 11.29 g/L pinoresinol. The molar yield and productivity were 52.77% and 1.03 g/(L × h), respectively. The elegant strategy developed herein utilizes the harmful by‐product to drive the biosynthetic reaction forward and simultaneously detoxify cells, thereby preventing enzyme inhibition. Biotechnol. Bioeng. 2017;114: 2066–2074. © 2017 Wiley Periodicals, Inc.
Fast and reliable strain characterization ofStreptomyces lividansthrough micro‐scale cultivation
Authors: Koepff Joachim , Keller Matthias , Tsolis Konstantinos C. , Busche Tobias , Rückert Christian , Hamed Mohamed B. , Anné Jozef , Kalinowski Joern , Wiechert Wolfgang , Economou Anastassios , Oldiges Marco 
Published: April 2017

ABSTRACTFilamentous organisms of the genusStreptomycesplay an important role in industrial production processes, due to their extensive secondary metabolism variability, as well as their ability to secrete efficiently large amounts of (heterologous) proteins. While genetic engineering tools are available to rapidly build up large strain libraries, the subsequent strain screening and bioprocess development still constitutes a bottleneck. This is due to the lack of reliable parallelized and accelerated cultivation techniques for morphologically challenging organisms. To address this challenge, we developed an integrated cultivation workflow forStreptomyces lividansbased on a parallelized shaken 48‐well microtiter‐plate (MTP) cultivation device. In a first step, a feasible pre‐culture method was identified and validated, revealing high comparability in subsequent main cultivations (coefficient of variation of 1.1% for in‐plate replicates and 3.2% between different pre‐cultures). When validating the growth performance in 1 mL MTP cultivation against an established 1,000 mL lab‐scale cultivation system, highly comparable cultivation patterns were found for online (pH, dissolved oxygen), as well as for offline derived parameters (glucose uptake, cell‐dry‐weight, and pellet size). Additionally, the two cultivation regimes were compared with respect to transcriptional and protein secretion activity ofStreptomyces, showing overall good comparability with minor, but well explainable discrepancies, most probably caused by different energy dissipation (shaking vs. stirring) and adaption effects due to different illumination conditions. Embedded within the presented cultivation workflow, the 1 mL MTP‐based parallelized cultivation system seems to be a suitable screening tool for filamentous and industrial relevant organisms likeStreptomyces. This can contribute to widen the field of application for these organisms and facilitate screening and early‐stage bioprocess development. Biotechnol. Bioeng. 2017;114: 2011–2022. © 2017 Wiley Periodicals, Inc.
Surgical and medical treatment of ocular disease in a dog with Ehlers–Danlos syndrome
Authors: Rasch Søren N. 
Published: April 2017

Key Clinical MessageCorrectional surgery was performed on a 3‐year‐old intact male shih tzu presenting with Ehlers–Danlos syndrome, ocular disease, and skin fold dermatitis. A one‐year follow‐up showed that no further clinical corrections were needed. Therefore, surgery could be considered in some canine patients with Ehlers–Danlos syndrome.
Diffuse hepatic hemangioma with single cutaneous hemangioma: an alerting occurrence
Authors: Al Tasseh Faten , El‐Khansa Merna , Abd Omran , Abdel Khalek Anoir , El‐Rifai Nahida 
Published: April 2017

Key Clinical MessageScreening of infants with five or more cutaneous infantile hemangiomas with abdominal ultrasound is often recommended. However, diffuse hepatic hemangioma can develop even in patients with single cutaneous hemangioma. This highlights the importance of physical examination and screening ultrasound in these patients.
Obinutuzumab‐induced serum sickness following salvage therapy for chronic lymphocytic leukemia
Authors: Saba Julie , Logan Aaron C. 
Published: April 2017

Key Clinical MessageThe incidence of serum sickness following treatment of CLL with obinutuzumab has not been fully characterized, but is likely rare. Consideration should be given to this diagnosis in appropriate circumstances so that effective corticosteroid therapy can be initiated to alleviate inflammatory symptoms and organ dysfunction in a timely manner.
Successful bailout stenting strategy against lethal coronary dissection involving left main bifurcation
Authors: Kubota Hiroshi , Nomura Tetsuya , Hori Yusuke , Yoshioka Kenichi , Miyawaki Daisuke , Urata Ryota , Sugimoto Takeshi , Kikai Masakazu , Keira Natsuya , Tatsumi Tetsuya 
Published: April 2017

Key Clinical MessageCatheter‐induced coronary dissection involving left main bifurcation is a rare complication during cardiac catheterization but can become lethal unless it is treated appropriately. Interventional cardiologists always have to pay attention to the risk of complications related to cardiac catheterization and prepare for determining the best bailout strategy for the situation.
Concomitant imatinib and ibrutinib in a patient with chronic myelogenous leukemia and chronic lymphocytic leukemia
Authors: Shea Lauren K. , Mikhail Fady M. , Forero‐Torres Andres , Davis Randall S. 
Published: April 2017

Key Clinical MessageThe availability of kinase and other small‐molecule inhibitors to treat hematologic malignancies is increasing. Accordingly, novel regimens that employ these therapeutics are rapidly evolving. Herein we report the safe and effective administration of two targeted kinase inhibitors in a patient with concomitant chronic myelogenous leukemia and chronic lymphocytic leukemia.
Semirational Engineering Accelerates the Laboratory Evolution of Nitrilase Catalytic Efficiency for Nicotinic Acid Biosynthesis
Authors: Gong Jin‐Song , Dong Ting‐Ting , Gu Bing‐Chen , Li Heng , Dou Wen‐Fang , Lu Zhen‐Ming , Zhou Zhe‐Min , Shi Jin‐Song , Xu Zheng‐Hong 
Published: April 2017

AbstractThe nitrilase‐mediated green synthesis of nicotinic acid, an important reaction in synthetic organic chemistry, has attracted considerable attention in recent years. However, the application potential of nitrilase is hindered by several limitations, such as low catalytic efficiency and byproduct formation. In this study, the site‐saturation mutagenesis of asparagine 40, phenylalanine 50, and glutamine 207 in recombinant nitrilase from Pseudomonas putida CGMCC3830 was conducted to improve the specificity of nitrilase for nicotinic acid. The resulting mutants, which contain the mutations N40G (asparagine→glycine), F50W (phenylalanine→tryptophan), and Q207E (glutamine→glutamic acid), produced higher nicotinic acid yields than the wild type. Furthermore, double and triple mutations were introduced, and four mutants that contained N40G/F50W, N40G/Q207E, F50W/Q207E, and N40G/F50W/Q207E were obtained and evaluated for their capacity to produce nicotinic acid. The double mutant F50W/Q207E and the triple mutant N40G/F50W/Q207E displayed the highest activity, which was nearly twofold higher than that of the wild type. The kinetics analysis of nicotinic acid synthesis with the mutant nitrilase revealed the higher catalytic capability of all the mutants than the wild type. These results provide new insights into the catalytic performance of P. putida nitrilase toward 3‐cyanopyridine to promote nicotinic acid production.
2D Layered α‐Fe2O3/rGO Flexible Electrode Prepared through Colloidal Electrostatic Self‐Assembly
Authors: Zhang Huifang , Lu Chunxiang , Chen Chengmeng , Xie Lijing , Zhou Pucha , Kong Qingqiang 
Published: April 2017

AbstractThrough the simple hydrolysis of FeCl3 in boiling water, Fe(OH)3 colloidal nanoparticles (NPs) can be achieved that exhibit a highly positively charge. Graphene oxide (GO) nanosheets are negatively charged when dispersed in water. Inspired by these fascinating characteristics, a 2D layered α‐Fe2O3/rGO hybrid film (FGHF) was fabricated through colloidal electrostatic self‐assembly with subsequent vacuum filtration and hydrothermal reduction. In this hybrid film, cubical α‐Fe2O3 NPs are well‐distributed on rGO sheets, which can provide sufficient active interfacial sites and effectively alleviate the volume variation of α‐Fe2O3 NPs during electrochemical reactions. The as‐obtained FGHF exhibits a high specific capacitance of 714 F g−1 and an outstanding rate capability of 42.6 % retention at 30 A g−1. Moreover, a flexible supercapacitor was fabricated, which can achieve a high volumetric capacitance of 16.45 F cm−3, leading to an energy density of 1.46 mWh cm−3 and a power density of 199.8 mW cm−3. The flexible nature of the FGHF allows the as‐fabricated supercapacitor to remain undamaged with almost no change in capacitive performance when bending up to 180°.
10‐Silacorroles Exhibiting Near‐Infrared Absorption and Emission
Authors: Omori Hiroto , Hiroto Satoru , Shinokubo Hiroshi 
Published: April 2017

Abstract10‐Silacorroles were obtained from the Pd‐catalyzed silylative cyclization of a bis(α,α′‐dibromodipyrrin) NiII precursor with dihydrosilanes. These 10‐silacorroles show substantially red‐shifted absorption bands relative to those of normal porphyrins and isocorroles. Notably, the corresponding free base and ZnII 10‐silacorroles exhibit emissions in the NIR region. Theoretical calculations on these 10‐silacorroles revealed the presence of σ*–π* conjugation between the silyl group and the tetrapyrrole π system, which significantly lowers their LUMO energy levels.
Chiral Fidelity in the Diastereoselective and Enantiospecific Synthesis of Indenes from Axially Chiral Benzylidene Cyclanes
Authors: Mouysset Dominique , Tessonnier Cyril , Tintaru Aura , Dumur Frédéric , Jean Marion , Vanthuyne Nicolas , Bertrand Michèle P. , Siri Didier , Nechab Malek 
Published: April 2017

AbstractEnantioenriched indenes were reached through a chirality conversion strategy using original axially chiral benzylidene cyclanes. Good to high remote diastereocontrol and excellent enantiocontrol were observed in this cascade involving copper‐catalyzed homologation of terminal alkynes, in situ allenoate formation and Alder‐ene cyclization.
Luminescent Ni0 Diisocyanide Chelates as Analogues of CuI Diimine Complexes
Authors: Büldt Laura A. , Larsen Christopher B. , Wenger Oliver S. 
Published: April 2017

AbstractInvited for the cover of this issue is the group of Oliver S. Wenger at the University Basel. The image depicts a homoleptic Ni0 diisocyanide complex that displays photoluminescence at low temperatures. Read the full text of the article at 10.1002/chem.201700103.
Is prostate cancer different in black men? Answers from 3 natural history models
Authors: Tsodikov Alex , Gulati Roman , de Carvalho Tiago M. , Heijnsdijk Eveline A. M. , Hunter‐Merrill Rachel A. , Mariotto Angela B. , de Koning Harry J. , Etzioni Ruth 
Published: April 2017

BACKGROUNDBlack men in the United States have substantially higher prostate cancer incidence rates than the general population. The extent to which this incidence disparity is because prostate cancer is more prevalent, more aggressive, and/or more frequently diagnosed in black men is unknown.METHODSThe authors estimated 3 independently developed models of prostate cancer natural history in black men and in the general population using an updated reconstruction of prostate‐specific antigen screening, based on the National Health Interview Survey in 2005 and on prostate cancer incidence data from the Surveillance, Epidemiology, and End Results program during 1975 through 2000. By using the estimated models, the natural history of prostate cancer was compared between black men and the general population.RESULTSThe models projected that from 30% to 43% (range across models) of black men develop preclinical prostate cancer by age 85 years, a risk that is (relatively) 28% to 56% higher than that in the general population. Among men who had preclinical disease onset, black men had a similar risk of diagnosis (range, 35%‐49%) compared with the general population (32%‐44%), but their risk of progression to metastatic disease by the time of diagnosis was from 44% to 75% higher than that in the general population.CONCLUSIONSProstate cancer incidence patterns implicate higher incidence of preclinical disease and higher risk of metastatic progression among black men. The findings suggest screening black men earlier than white men and support further research into the benefit‐harm tradeoffs of more aggressive screening policies for black men. Cancer 2017;123:2312–2319. © 2017 American Cancer Society.
Histiocytic sarcoma: New insights into FNA cytomorphology and molecular characteristics
Authors: Hung Yin P. , Lovitch Scott B. , Qian Xiaohua 
Published: April 2017

BACKGROUNDHistiocytic sarcoma (HS) is a rare malignant neoplasm showing morphologic and immunophenotypic features of histiocytes. Molecular characteristics of HS and fine‐needle aspiration (FNA) criteria for its diagnosis have not been established.METHODSA case series of HS in 8 FNA samples from 6 patients was reviewed along with histopathologic and clinical data. Immunohistochemistry was performed on cell blocks (3 cases), core biopsies (5 cases), and surgical specimens (4 cases). Targeted‐exome next‐generation sequencing (NGS) was performed on surgical resection specimens in 4 cases.RESULTSFour patients had a known history of hematolymphoid malignancy. Cytomorphologic features included variably cellular smears composed of large epithelioid cells with reniform nuclei and abundant vacuolated cytoplasm, in an inflammatory background, with occasional cytophagocytosis and lymphoglandular bodies. Marked pleomorphism, multinucleated monster cells, and binucleated histiocytoid cells with partially overlapping, eccentrically placed nuclei resembling Pac‐Man were common. Most cases expressed histiocytic markers CD68 (6 of 7 cases), CD163 (5 of 5 cases), and PU.1 (3 of 4 cases). In 3 cases, NGS analysis revealed alterations in lysine methyltransferase 2D (KMT2D)/mixed‐lineage leukemia 2 (MLL2), a gene involved in chromatin regulation and previously implicated in the pathogenesis of follicular lymphoma.CONCLUSIONSAlthough diagnosing HS with FNA alone is extremely challenging, the presence of pleomorphic and epithelioid large cells with binucleation and/or multinucleation in an inflammatory background should prompt the diagnosis of HS with judicious use of confirmatory histiocytic lineage markers. The detection of recurrent KMT2D/MLL2 alterations implicates epigenetic regulation in the pathogenesis of HS and supports the notion of transdifferentiation from a genetically similar but phenotypically distinct tumor of a different lineage. Cancer Cytopathol 2017;125:604‐14. © 2017 American Cancer Society.
Particle swarm optimization–deep belief network–based rare class prediction model for highly class imbalance problem
Authors: Kim Jae Kwon , Han Young Shin , Lee Jong Sik 
Published: April 2017

SummaryRare class imbalance problems, which involve the classification of minority or rare class, are difficult, because the size of the rare class is smaller than the majority class. Since majority class prediction is easy, its accuracy seems to be also high. However, the minority classes cannot be accurately predicted, and for this reason, when the prediction model performance is evaluated by considering only the accuracy, it does not indicate whether the model can predict the minority classes. Therefore, a rare class prediction technique is required. In this study, a rare class prediction model is proposed for minority class prediction. In addition, a dataset of a semiconductor manufacturing process with class imbalance problems was used to create a fault detection model. This prediction model uses data preprocessing to build the characteristics and data set required by the rare classes. To distinguish the rare classes related to the required characteristics, we used standard deviation and Euclidean distance to perform the feature selection. In addition, a particle swarm optimization–deep belief network was applied to create a classifier. The model proposed in this research presents outstanding performance and is appropriate for highly class imbalance problems.
Interactive museum exhibits with embedded systems: A use‐case scenario
Authors: Wong Lok , Shimojo Shinji , Teranishi Yuuichi , Yoshihisa Tomoki , Haga Jason H. 
Published: April 2017

SummaryThe feasibility of using embedded systems in real‐life applications is becoming more widespread. These applications have grown from do‐it‐yourself projects of computer enthusiasts or robotics projects to larger scale efforts and deployments. This paper describes a scenario that deployed a prototype application that allows the public to interact with features of a model and view videos from a first‐person perspective on the train. Through testing the embedded systems and their usage in a public setting, it was demonstrated that interactive features could be implemented in model train exhibits, which are featured in traditional museum environments that lack technical infrastructure. Specifically, the Arduino and Raspberry Pi provide the necessary linkages between the Internet and hardware, allowing for a greater interactive experience for museum visitors. These results provide an important use‐case scenario and lessons learned that cultural heritage institutions can use when implementing embedded systems on a larger scale, for the purpose of increasing visitors' experience through greater interaction and engagement.
Integration of analytic network process with service measurement index framework for cloud service provider selection
Authors: Tripathi Atul , Pathak Isha , Vidyarthi Deo Prakash 
Published: April 2017

SummaryThe growing popularity of cloud services has attracted large number of business organizations to enter into the cloud market. The varied availability of cloud service providers, for various services, leaves the cloud customers into skepticism. Service measurement index (SMI) framework, which is based on the analytic hierarchical process, is developed for the ranking of cloud services. As there are multiple dependencies among the criteria for the selection of cloud service provider, analytic hierarchical process formulation is not efficient. In the proposed work, analytic network process that captures the cloud service‐provider selection problem in a more realistic way is integrated in the ranking component of SMI framework. In this method, interactions among the criteria are used for the ranking of the cloud services. Few new criterions are also added into the existing SMI framework to make the framework more effective. A case study is conducted to analyze the proposed technique for the cloud provider selection.
Solvent‐Free Mechanochemical Synthesis of Nitrogen‐Doped Nanoporous Carbon for Electrochemical Energy Storage
Authors: Schneidermann Christina , Jäckel Nicolas , Oswald Steffen , Giebeler Lars , Presser Volker , Borchardt Lars 
Published: April 2017

AbstractNitrogen‐doped nanoporous carbons were synthesized by a solvent‐free mechanochemically induced one‐pot synthesis. This facile approach involves the mechanochemical treatment and carbonization of three solid materials: potassium carbonate, urea, and lignin, which is a waste product from pulp industry. The resulting nitrogen‐doped porous carbons offer a very high specific surface area up to 3000 m2 g−1 and large pore volume up to 2 cm3 g−1. The mechanochemical reaction and the impact of activation and functionalization are investigated by nitrogen and water physisorption and high‐resolution X‐ray photoelectron spectroscopy (XPS). Our N‐doped carbons are highly suitable for electrochemical energy storage as supercapacitor electrodes, showing high specific capacitances in aqueous 1 m Li2SO4 electrolyte (177 F g−1), organic 1 m tetraethylammonium tetrafluoroborate in acetonitrile (147 F g−1), and an ionic liquid (1‐ethyl‐3‐methylimidazolium tetrafluoroborate; 192 F g−1). This new mechanochemical pathway synergistically combines attractive energy‐storage ratings with a scalable, time‐efficient, cost‐effective, and environmentally favorable synthesis.
Male clinical norms and sex differences on the Eating Disorder Inventory (EDI) and Eating Disorder Examination Questionnaire (EDE‐Q)
Authors: Smith Kathryn E. , Mason Tyler B. , Murray Stuart B. , Griffiths Scott , Leonard Rachel C. , Wetterneck Chad T. , Smith Brad E. R. , Farrell Nicholas R. , Riemann Bradley C. , Lavender Jason M. 
Published: April 2017

AbstractObjectiveEvidence indicates that males account for a significant minority of patients with eating disorders (EDs). However, prior research has been limited by inclusion of small and predominantly non‐clinical samples of males. This study aimed to (1) provide male clinical norms for widely used ED measures (Eating Disorder Examination Questionnaire [EDE‐Q] and Eating Disorder Inventory‐3 [EDI‐3]) and (2) examine sex differences in overall ED psychopathology.MethodParticipants were 386 male and 1,487 female patients with an ED diagnosis aged 16 years and older who completed the EDE‐Q and EDI‐3 upon admission to a residential or partial hospital ED treatment program.ResultsNormative data were calculated for the EDE‐Q (global and subscales) and the EDI‐3 (drive for thinness, body dissatisfaction, and bulimia). Analyses of variance (ANOVAs) used to examine sex, ED diagnosis, and their interaction in relation to overall ED psychopathology revealed a consistent pattern of greater severity among females for ED psychopathology.DiscussionThis study provides clinical norms on the EDE‐Q and the EDI‐3 for males with clinically diagnosed EDs. It is unclear whether the greater severity observed in females reflects qualitative differences in ED presentation or true quantitative differences in ED severity. Additional research examining the underlying nature of these differences and utilizing male‐specific ED measures with clinical samples is warranted.