bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mass spectrometry analysis and in silico prediction of allergenicity of peptides in tryptic hydrolysates of the proteins from Ruditapes philippinarum
Authors: Yu Yue , Liu Hongwei , Tu Maolin , Qiao Meiling , Wang Zhenyu , Du Ming 
Published: April 2017

AbstractBACKGROUNDRuditapes philippinarum is nutrient‐rich and widely‐distributed, but little attention has been paid to the identification and characterization of the bioactive peptides in the bivalve. In the present study, we evaluated the peptides of the R. philippinarum that were enzymolysised by trypsin using a combination of ultra‐performance liquid chromatography separation and electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry, followed by data processing and sequence‐similarity database searching. The potential allergenicity of the peptides was assessed in silico.RESULTSThe enzymolysis was performed under the conditions: E:S 3:100 (w/w), pH 9.0, 45 °C for 4 h. After separation and detection, the Swiss‐Prot database and a Ruditapes philippinarum sequence database were used: 966 unique peptides were identified by non‐error tolerant database searching; 173 peptides matching 55 precursor proteins comprised highly conserved cytoskeleton proteins. The remaining 793 peptides were identified from the R. philippinarum sequence database. The results showed that 510 peptides were labeled as allergens and 31 peptides were potential allergens; 425 peptides were predicted to be nonallergenic.CONCLUSIONThe abundant peptide information contributes to further investigations of the structure and potential function of R. philippinarum. Additional in vitro studies are required to demonstrate and ensure the correct production of the hydrolysates for use in the food industry with respect to R. philippinarum. © 2017 Society of Chemical Industry
Some posterior branches of extralaryngeal recurrent laryngeal nerves have motor fibers
Authors: Cho Ilyoung , Jo Min‐Gyu , Choi Sung‐Won , Jang Jeon Yeob , Wang Soo‐Geun , Cha Wonjae 
Published: April 2017

Objectives/HypothesisAnatomical variations of the recurrent laryngeal nerve (RLN), such as extralaryngeal branching, are a well‐known risk factor for RLN injury during thyroid surgery. This study aimed to analyze the surgical anatomy and to investigate the existence of posterior branch motor fibers of extralaryngeal RLNs.Study DesignProspective consecutive observational study.MethodsThis was a prospective cohort study of 366 patients between January 2014 and February 2016. Operative data included the type of operation, incidence of nerve bifurcation, the distances among anatomical landmarks. The motor fibers were evaluated using neurostimulation with laryngeal palpation.ResultsA total of 667 RLNs at risk were analyzed in this study, and of these 103 (14.5%) nerves were bifurcated or trifurcated before the laryngeal entry point (LEP). More extralaryngeal branched RLNs were observed on the right side than on the left (17.5% vs. 13.3%, P = .294). The mean distance of the LEP point of division was longer on the left side (16.2 ± 6.7 mm) than on the right (14.7 ± 5.9 mm, P = .132). All branched RLNs had a palpable laryngeal twitch when stimulating anterior branches. When stimulating posterior branches, 28.2%(29/103) of branched RLNs showed palpable laryngeal twitch. Overall incidence of posterior motor branch in total RLNs was 4.3% (29/667).ConclusionsThe motor fibers of the RLN are all located in the anterior branch, whereas some posterior branches have motor function. Identification of all of the branches of the RLN may be mandatory to decrease the risk of postoperative nerve injury.Level of Evidence4. Laryngoscope, 127:2678–2685, 2017
Dynamic pricing policies for an inventory model with random windows of opportunities
Authors: den Boer Arnoud , Perry Ohad , Zwart Bert 
Published: April 2017

AbstractWe study a single‐product fluid‐inventory model in which the procurement price of the product fluctuates according to a continuous time Markov chain. We assume that a fixed order price, in addition to state‐dependent holding costs are incurred, and that the depletion rate of inventory is determined by the sell price of the product. Hence, at any time the controller has to simultaneously decide on the selling price of the product and whether to order or not, taking into account the current procurement price and the inventory level. In particular, the controller is faced with the question of how to best exploit the random time windows in which the procurement price is low. We consider two policies, derive the associated steady‐state distributions and cost functionals, and apply those cost functionals to study the two policies.© 2017 Wiley Periodicals, Inc. Naval Research Logistics 65: 660–675, 2018
Competing structures in (In,Ga)Se and (In,Ga)2Se3 semiconductors
Authors: Srour J. , Postnikov A. , Badawi M. , El Haj Hassan F. 
Published: April 2017

AbstractThe electronic structure of four polytypes (, , , and ) of hexagonal GaSe and InSe is calculated from first principles, using the WIEN2k and VASP codes and PBEsol prescription for the exchange–correlation potential, aiming specifically at elucidating the crystallographic parameters and comparing the energy placement of corresponding competing structures. Further on, the compounds with different composition of the same constituents, namely the ordered‐vacancies systems GaSe and InSe were subject to a similar study, of which the relaxed crystal structure data for three different phases is reported. Comparison is done with the nominal wurtzite structure over which the cation vacancies are introduced, and the relaxation pattern discussed.
Universal vs selective ultrasonography to screen for large‐for‐gestational‐age infants and associated morbidity
Authors: Sovio U. , Moraitis A. A. , Wong H. S. , Smith G. C. S. 
Published: April 2017

ABSTRACTObjectivesTo compare the diagnostic effectiveness of selective vs universal ultrasonography as a screening test for large‐for‐gestational‐age (LGA) infants, and to determine whether previously described ultrasound markers of excessive fetal growth could identify suspected LGA fetuses that are at increased risk of adverse neonatal outcome.MethodsData from the Pregnancy Outcome Prediction study, a prospective cohort study of nulliparous women with a viable singleton pregnancy at the time of the dating ultrasound scan, were analyzed. Women were selected for clinically indicated ultrasound assessment in the third trimester as per routine clinical care, and the results of these scans were reported (‘selective ultrasonography’). In addition, all participants underwent research ultrasound scans, including estimated fetal weight (EFW) assessment, at around 36 weeks' gestation, in which both the women and their clinicians were blinded to the results (‘universal ultrasonography’). Participants who attended the 36‐week research scan and had a live birth at the Rosie Hospital were included in the study. Screen positive for LGA was defined as EFW > 90th percentile at ≥ 34 weeks.ResultsThe current analysis included 3866 eligible women, of whom 1354 (35%) had a clinically indicated ultrasound scan at or after 34 weeks' gestation. A total of 177 (4.6%) infants had a birth weight > 90th percentile. The sensitivity for detection of LGA infants was 27% for selective ultrasonography and 38% for universal ultrasonography. The specificity of both approaches was high (99% and 97%, respectively). Using universal ultrasonography, neonatal outcome differed (P for interaction) by abdominal circumference growth velocity (ACGV) for both any neonatal morbidity (P = 0.08) and severe adverse neonatal outcome (P = 0.03). LGA fetuses with increased ACGV had a relative risk of any neonatal morbidity of 2.0 (95% CI, 1.1–3.6; P = 0.04) and of severe adverse neonatal outcome of 6.5 (95% CI, 2.0–21.1; P = 0.01), whereas LGA fetuses with normal ACGV were not at increased risk.ConclusionsThird‐trimester screening of nulliparous women by universal ultrasound fetal biometry increases the detection rate of LGA infants and, combined with ACGV, identifies those at increased risk of adverse neonatal outcome. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.
A History of Reading and Writing
Authors: Lyons Martyn 
Published: April 2017

Martyn Lyons surveys the changing relationships enjoyed by men and women with the written word, from early times to the present day. He provides a highly-readable account of the social history of reading and writing, relating it to key historical moments such as the Renaissance, the Reformation and the Enlightenment.
Offering a fresh history centred on the reactions and experiences of ordinary readers and writers, Lyons deals with key turning points that occurred throughout the centuries, such as the invention of the codex, the transition from scribal to print culture, the reading revolution and the industrialisation of the book. Tracing the major historical developments across Europe and North America which revolutionised our relationship with texts, this book provides an engaging and invaluable overview of the history of scribal and print culture.
History of anticlinal traps and oil and gas fields in Jurassic reservoirs in the northern West Siberian basin
Authors: Pervukhina N.V. , Shemin G.G. , Moskvin V.I. 
Published: April 2017

Abstract
The paper presents new paleotectonic data on the Bovanenkovo, Taz, and Gubkin dome-shaped uplifts and the Novyi Port, Urengoi, Kharampur, and Medvezh’e swells, with implications for the history of zone J2 and the Jurassic reservoir as a whole. Most of the large uplifts originated in the Early–Middle Jurassic and completed their evolution in the early Late Cretaceous (latest Cenomanian), generally (Bovanenkovo, Novyi Port, Kharampur, and Taz) or to a large extent (Urengoi and Gubkin), except for the much younger Medvezh’e swell, which formed in the Paleogene–Quaternary interval. The conditions for oil storage and retention in anticlinal traps of Jurassic zones J1 and J2 were the most favorable between the middle Late Cretaceous and the Eocene, inclusive. Gas accumulation lasted for a much longer time, from the Late Jurassic to the Present, and was especially rapid in the Late Cretaceous–Paleogene.
Dynamic associations of network isolation and smoking behavior
Authors: COPELAND MOLLY , BARTLETT BRYCE , FISHER JACOB C. 
Published: April 2017

AbstractPrevailing social network frameworks examine the association between peer ties and behaviors, such as smoking, but the role of social isolates is poorly understood. Some theories predict isolated adolescents are protected from peer influence that increases smoking, while others suggest isolates are more likely to initiate smoking because they lack the social control provided by peer friendships. Building on a growing literature that seeks to explain these contradictions by moving beyond a homogeneous understanding of isolation, we identify the relationship between smoking and three distinct dimensions of isolation: avoided (adolescents who do not receive ties), withdrawn (adolescents who do not send ties), and externally oriented (adolescents who claim close out-of-grade friends). We examine the co-evolutionary effects of these dimensions and cigarette smoking using an autoregressive latent trajectory model with PROSPER Peers, a unique, longitudinal network dataset. These data include students (47% male and 86% white) from rural Iowa and Pennsylvania, ranging successively from grades 6–12 in eight waves of data. We find avoided isolation is associated with decreased subsequent smoking in high school. Smoking increases subsequent avoided and withdrawn isolation, but decreases external orientation.
Gaining insights into the ecological role of the New Zealand sole (Peltorhamphus novaezeelandiae) through parasites
Authors: Anglade T. , Randhawa H.S. 
Published: April 2017

AbstractDespite the fact that tapeworms comprise the bulk of parasite communities of sharks in marine ecosystems, little is known about their life cycles and, more specifically, about the potential intermediate hosts they utilize as transmission routes. In the absence of morphological features required for specific identification of larval tapeworms from potential intermediate hosts, recent molecular advances have contributed to linking larval and adult parasites and, in some instances, uncovering unknown trophic links. Host–parasite checklists are often the first source of information consulted to assess the diversity and host specificity of parasites, and provide insights into parasite identification. However, these host–parasite checklists are only useful if they encompass the full spectrum of associations between hosts and parasites. A checklist of New Zealand fishes and their parasites has been published, but recent parasitological examinations of commercial fish species reveal that the checklist appears to be far from complete. We focused our current study on a comprehensive survey of macroparasites of a commercial species, the New Zealand sole (Peltorhamphus novaezeelandiae) off the coast of Otago, New Zealand. Specifically, we were expecting to recover marine tapeworms using sharks as their definitive hosts that are generally underreported in parasite surveys. The parasites recovered included tapeworms, flukes, round worms and thorny-headed worms. Surprisingly, a large proportion of the non-tapeworm parasites we recovered were not previously reported from this fish species. A discussion on the potential ecological roles played by this fish species in the transmission of parasites is included.
Olfactory disturbances in ageing with and without dementia: towards new diagnostic tools
Authors: Gros A , Manera V , De March C A , Guevara N , König A , Friedman L , Robert P , Golebiowski J , David R 
Published: April 2017

AbstractBackground:Olfactory disorders increase with age and often affect elderly people who have pre-dementia or dementia. Despite the frequent occurrence of olfactory changes at the early stages of neurodegenerative disorders such as Alzheimer's disease, olfactory disorders are rarely assessed in daily clinical practice, mainly due to a lack of standardised assessment tools. The aims of this review were to (1) summarise the existing literature on olfactory disorders in ageing populations and patients with neurodegenerative disorders; (2) present the strengths and weaknesses of current olfactory disorder assessment tools; and (3) discuss the benefits of developing specific olfactory tests for neurodegenerative diseases.Methods:A systematic review was performed of literature published between 2000 and 2015 addressing olfactory disorders in elderly people with or without Alzheimer's disease or other related disorders to identify the main tools currently used for olfactory disorder assessment.Results:Olfactory disorder assessment is a promising method for improving both the early and differential diagnosis of Alzheimer's disease. However, the current lack of consensus on which tests should be used does not permit the consistent integration of olfactory disorder assessment into clinical settings.Conclusion:Otolaryngologists are encouraged to use olfactory tests in older adults to help predict the development of neurodegenerative diseases. Olfactory tests should be specifically adapted to assess olfactory disorders in Alzheimer's disease patients.
The cellular senescence of leukemia-initiating cells from acute lymphoblastic leukemia is postponed by β-Arrestin1 binding with P300-Sp1 to regulate hTERT transcription
Authors: Liu Shan , Liu Haiyan , Qin Ru , Shu Yi , Liu Zhidai , Zhang Penghui , Duan Caiwen , Hong Dengli , Yu Jie , Zou Lin 
Published: April 2017

AbstractAlthough we previously reported that the self-renewal of leukemia-initiating cells of B-lineage acute lymphoblastic leukemia (B-ALL LICs) was regulated by β-Arrestin1, a multiple-function protein, the cellular senescence is critical for LICs fate and leukemia progress, and worthy for further investigation. Here we found that depletion of β-Arrestin1 extended the population doubling time and the percentage of senile cells, the signatures of cellular senescence, of B-ALL LICs. Moreover, lack of β-Arrestin1 enhanced the expression of proteins (CBX, HIRA) and genes (P53, P16) related to senescence in leukemic Reh cells and B-ALL-LICs-derived leukemic mice. Further results showed that loss of β-Arrestin1 induced senescence of Reh cells through mediating hTERT-telomerase-telomere axis, which was reversed by BIBR1532, the telomerase activity inhibitor. Importantly, depletion of β-Arrestin1 decreased the binding of Sp1 to hTERT promoter at the region of −28 to −36 bp. The anti-sense oligonucleotide of this key region downregulated the transcription of hTERT and aggravated the senescence of Reh cells. Further data demonstrated that the depleted β-Arrestin1 reduced the interaction of P300 with Sp1, thus to reduce Sp1 binding to hTERT promoter, downregulate hTERT transcription, decrease telomerase activity, shorten telomere length, and promote Reh cell senescence. Interestingly, the percentage of senile cells in B-ALL LICs was decreased, which was negatively correlated to good prognosis and β-Arrestin1 mRNA expression in childhood B-ALL patients. Our study shed a light on the senescence of B-ALL LICs and is regulated by β-Arrestin1, providing the potential therapeutic target of leukemia by promoting cellular senescence with a key region of hTERT promoter.
Activation of the hypothalamic feeding centre upon visual prey detection
Authors: Muto Akira , Lal Pradeep , Ailani Deepak , Abe Gembu , Itoh Mari , Kawakami Koichi 
Published: April 2017

AbstractThe visual system plays a major role in food/prey recognition in diurnal animals, and food intake is regulated by the hypothalamus. However, whether and how visual information about prey is conveyed to the hypothalamic feeding centre is largely unknown. Here we perform real-time imaging of neuronal activity in freely behaving or constrained zebrafish larvae and demonstrate that prey or prey-like visual stimuli activate the hypothalamic feeding centre. Furthermore, we identify prey detector neurons in the pretectal area that project to the hypothalamic feeding centre. Ablation of the pretectum completely abolishes prey capture behaviour and neurotoxin expression in the hypothalamic area also reduces feeding. Taken together, these results suggest that the pretecto-hypothalamic pathway plays a crucial role in conveying visual information to the feeding centre. Thus, this pathway possibly converts visual food detection into feeding motivation in zebrafish.
Histamine and Histamine H4 Receptor Promotes Osteoclastogenesis in Rheumatoid Arthritis
Authors: Kim Kyoung-Woon , Kim Bo-Mi , Lee Kyung-Ann , Lee Sang-Heon , Firestein Gary S. , Kim Hae-Rim 
Published: April 2017

AbstractHistamine H4 receptor (H4R) has immune-modulatory and chemotaxic effects in various immune cells. This study aimed to determine the osteoclastogenic role of H4R in rheumatoid arthritis (RA). The concentration of histamine in synovial fluid (SF) and sera in patients with RA was measured using ELISA. After RA SF and peripheral blood (PB) CD14+ monocytes were treated with histamine, IL-17, IL-21 and IL-22, and a H4R antagonist (JNJ7777120), the gene expression H4R and RANKL was determined by real-time PCR. Osteoclastogenesis was assessed by counting TRAP–positive multinucleated cells in PB CD14+ monocytes cultured with histamine, Th17 cytokines and JNJ7777120. SF and serum concentration of histamine was higher in RA, compared with osteoarthritis and healthy controls. The expression of H4R was increased in PB monocytes in RA patients. Histamine, IL-6, IL-17, IL-21 and IL-22 induced the expression of H4R in monocytes. Histamine, IL-17, and IL-22 stimulated RANKL expression in RA monocytes and JNJ7777120 reduced the RANKL expression. Histamine and Th17 cytokines induced the osteoclast differentiation from monocytes and JNJ7777120 decreased the osteoclastogenesis. H4R mediates RANKL expression and osteoclast differentiation induced by histamine and Th17 cytokines. The blockage of H4R could be a new therapeutic modality for prevention of bone destruction in RA.
Delivering Prolonged Intensive Care to a Non-human Primate: A High Fidelity Animal Model of Critical Illness
Authors: Poliquin P. Guillaume , Biondi Mia , Ranadheera Charlene , Hagan Mable , Bello Alexander , Racine Trina , Allan Mark , Funk Duane , Hansen Gregory , Hancock BJ , Kesselman Murray , Mortimer Todd , Kumar Anand , Jones Shane , Leung Anders , Grolla Allen , Tran Kaylie N. , Tierney Kevin , Qiu Xiangguo , Kobasa Darwyn , Strong James E. 
Published: April 2017

AbstractCritical care needs have been rising in recent decades as populations age and comorbidities increase. Sepsis-related admissions to critical care contribute up to 50% of volume and septic shock carries a 35–54% fatality rate. Improvements in sepsis-related care and mortality would have a significant impact of a resource-intensive area of health care delivery. Unfortunately, research has been hampered by the lack of an animal model that replicates the complex care provided to humans in an intensive care unit (ICU). We developed a protocol to provide full ICU type supportive care to Rhesus macaques. This included mechanical ventilation, continuous sedation, fluid and electrolyte management and vasopressor support in response to Ebolavirus-induced septic shock. The animals accurately recapitulated human responses to a full range of ICU interventions (e.g. fluid resuscitation). This model can overcome current animal model limitations by accurately emulating the complexity of ICU care and thereby provide a platform for testing new interventions in critical care and sepsis without placing patients at risk.
Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption
Authors: O’Connell Grant C. , Treadway Madison B. , Petrone Ashley B. , Tennant Connie S. , Lucke-Wold Noelle , Chantler Paul D. , Barr Taura L. 
Published: April 2017

AbstractOur group recently identified 16 genes whose peripheral blood expression levels are differentially regulated in acute ischemic stroke. The purpose of this study was to determine whether the early expression levels of any of these 16 genes are predictive for post-stroke blood brain barrier (BBB) disruption. Transcriptional expression levels of candidate genes were measured in peripheral blood sampled from ischemic stroke patients at emergency department admission, and BBB permeability was assessed at 24 hour follow up via perfusion-weighted imaging. Early heightened expression levels of AKAP7, a gene encoding a protein kinase A-binding scaffolding molecule, were significantly associated with BBB disruption 24 hours post-hospital admission. We then determined that AKAP7 is predominantly expressed by lymphocytes in peripheral blood, and strongly co-expressed with ITGA3, a gene encoding the adhesion molecule integrin alpha 3. Subsequent in vitro experiments revealed that heightened expression of AKAP7 and ITGA3 in primary human lymphocytes is associated with a highly adherent phenotype. Collectively, our results suggest that AKAP7 expression levels may have clinical utility as a prognostic biomarker for post-stroke BBB complications, and are likely elevated early in patients who later develop post-stroke BBB disruption due to the presence of an invasive lymphocyte population in the peripheral blood.