bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nominalization and the problem of indirect access: Evidence from Ossetian
Authors: Lyutikova Ekaterina , Tatevosov Sergei 
Published: October 2016

AbstractWe examine aspect, eventuality type and transitivity alternations in two types of nominalization in Ossetian, an Iranian language spoken in East Caucasus, focusing on how deverbal nominals differ from fully inflected clauses projected by the same VPs. The main empirical finding of the study is: nominalizations exhibit a wider range of syntactic and semantic possibilities than fully inflected clauses. The finite verb in Ossetian is either perfective or imperfective and shows Slavic-type aspectual composition, but (prefixless) nominalizations are aspectless and are associated with English-type aspectual composition. Besides, deverbal nominals allow for transitivity alternations even if a corresponding finite verb does not. Our account for the observed pattern is based on the hypothesis that in the course of derivation, the range of options available at the VP level is narrowed down by the elements of functional structure, specifically, Asp and T. We argue that the role of Asp is to impose a quantization requirement on its complement predicate, while T interacts with the structure generated at the VP level in determining transitivity of a clause. Nominals can be built before Asp and T are merged, hence the semantic contribution of those heads is not part of their meaning. It that way, nominals provide us with a more direct access to the true characteristics of the verb and its immediate projections at early stages of derivation.
From Newspaper to Parliament and Back? A Study of Media Attention as Source for and Result of the Dutch Question Hour
Authors: Van Aelst Peter , van Santen Rosa , Melenhorst Lotte , Helfer Luzia 
Published: October 2016

Abstract
This study on the role of media attention for the Dutch question hour answers three questions: to what extent is media attention a source of inspiration for oral parliamentary questions? What explains the newsworthiness of these questions? And what explains the extent of media coverage for the questions posed during the question hour? To address this, we present a content analysis of oral parliamentary questions and related press coverage in five recent years. The results show first that oral questions are usually based on media attention for a topic. Concerns about media influence should however be nuanced: it is not necessarily the coverage itself, but also regularly a political statement that is the actual source of a parliamentary question. The media are thus an important “channel” for the interaction between politicians. Second, our analysis shows that oral questions do not receive media attention naturally. Several news values help to explain the amount of news coverage that questions receive. “Surfing the wave” of news attention for a topic in the days previous to the question hour seems to be the best way to generate media attention.
Thyroid Hormone Acts Locally to Increase Neurogenesis, Neuronal Differentiation, and Dendritic Arbor Elaboration in the Tadpole Visual System
Authors: Thompson Christopher K. , Cline Hollis T. 
Published: October 2016

Thyroid hormone (TH) regulates many cellular events underlying perinatal brain development in vertebrates. Whether and how TH regulates brain development when neural circuits are first forming is less clear. Furthermore, although the molecular mechanisms that impose spatiotemporal constraints on TH action in the brain have been described, the effects of local TH signaling are poorly understood. We determined the effects of manipulating TH signaling on development of the optic tectum in stage 46–49Xenopus laevistadpoles. Global TH treatment caused large-scale morphological effects in tadpoles, including changes in brain morphology and increased tectal cell proliferation. Either increasing or decreasing endogenous TH signaling in tectum, by combining targeted DIO3 knockdown and methimazole, led to corresponding changes in tectal cell proliferation. Local increases in TH, accomplished by injecting suspensions of tri-iodothyronine (T3) in coconut oil into the midbrain ventricle or into the eye, selectively increased tectal or retinal cell proliferation, respectively.In vivotime-lapse imaging demonstrated that local TH first increased tectal progenitor cell proliferation, expanding the progenitor pool, and subsequently increased neuronal differentiation. Local T3also dramatically increased dendritic arbor growth in neurons that had already reached a growth plateau. The time-lapse data indicate that the same cells are differentially sensitive to T3at different time points. Finally, TH increased expression of genes pertaining to proliferation and neuronal differentiation. These experiments indicate that endogenous TH locally regulates neurogenesis at developmental stages relevant to circuit assembly by affecting cell proliferation and differentiation and by acting on neurons to increase dendritic arbor elaboration.SIGNIFICANCE STATEMENTThyroid hormone (TH) is a critical regulator of perinatal brain development in vertebrates. Abnormal TH signaling in early pregnancy is associated with significant cognitive deficits in humans; however, it is difficult to probe the function of TH in early brain development in mammals because of the inaccessibility of the fetal brain in the uterine environment and the challenge of disambiguating maternal versus fetal contributions of TH. The external development of tadpoles allows manipulation and direct observation of the molecular and cellular mechanisms underlying TH's effects on brain development in ways not possible in mammals. We find that endogenous TH locally regulates neurogenesis at developmental stages relevant to circuit assembly by affecting neural progenitor cell proliferation and differentiation and by acting on neurons to enhance dendritic arbor elaboration.
Cancer‐specific mutations in p53 induce the translation of Δ160p53 promoting tumorigenesis
Authors: Candeias Marco M , Hagiwara Masatoshi , Matsuda Michiyuki 
Published: October 2016

AbstractWild‐type p53 functions as a tumour suppressor while mutant p53 possesses oncogenic potential. Until now it remains unclear how a single mutation can transform p53 into a functionally distinct gene harbouring a new set of original cellular roles. Here we show that the most common p53 cancer mutants express a larger number and higher levels of shorter p53 protein isoforms that are translated from the mutated full‐length p53 mRNA. Cells expressing mutant p53 exhibit “gain‐of‐function” cancer phenotypes, such as enhanced cell survival, proliferation, invasion and adhesion, altered mammary tissue architecture and invasive cell structures. Interestingly, Δ160p53‐overexpressing cells behave in a similar manner. In contrast, an exogenous or endogenous mutant p53 that fails to express Δ160p53 due to specific mutations or antisense knock‐down loses pro‐oncogenic potential. Our data support a model in which “gain‐of‐function” phenotypes induced by p53 mutations depend on the shorter p53 isoforms. As a conserved wild‐type isoform, Δ160p53 has evolved during millions of years. We thus provide a rational explanation for the origin of the tumour‐promoting functions of p53 mutations.
Gadd45a is a heterochromatin relaxer that enhances iPS cell generation
Authors: Chen Keshi , Long Qi , Wang Tao , Zhao Danyun , Zhou Yanshuang , Qi Juntao , Wu Yi , Li Shengbiao , Chen Chunlan , Zeng Xiaoming , Yang Jianguo , Zhou Zisong , Qin Weiwen , Liu Xiyin , Li Yuxing , Li Yingying , Huang Xiaofen , Qin Dajiang , Chen Jiekai , Pan Guangjin , Schöler Hans R , Xu Guoliang , Liu Xingguo , Pei Duanqing 
Published: October 2016

AbstractReprogramming of somatic cells to induced pluripotent stem cells rewrites the code of cell fate at the chromatin level. Yet, little is known about this process physically. Here, we describe a fluorescence recovery after photobleaching method to assess the dynamics of heterochromatin/euchromatin and show significant heterochromatin loosening at the initial stage of reprogramming. We identify growth arrest and DNA damage‐inducible protein a (Gadd45a) as a chromatin relaxer in mouse embryonic fibroblasts, which also enhances somatic cell reprogramming efficiency. We show that residue glycine 39 (G39) in Gadd45a is essential for interacting with core histones, opening chromatin and enhancing reprogramming. We further demonstrate that Gadd45a destabilizes histone–DNA interactions and facilitates the binding of Yamanaka factors to their targets for activation. Our study provides a method to screen factors that impact on chromatin structure in live cells, and identifies Gadd45a as a chromatin relaxer.
Drugging the addict: non‐oncogene addiction as a target for cancer therapy
Authors: Nagel Remco , Semenova Ekaterina A , Berns Anton 
Published: October 2016

AbstractHistorically, cancers have been treated with chemotherapeutics aimed to have profound effects on tumor cells with only limited effects on normal tissue. This approach was followed by the development of small‐molecule inhibitors that can target oncogenic pathways critical for the survival of tumor cells. The clinical targeting of these so‐called oncogene addictions, however, is in many instances hampered by the outgrowth of resistant clones. More recently, the proper functioning of non‐mutated genes has been shown to enhance the survival of many cancers, a phenomenon called non‐oncogene addiction. In the current review, we will focus on the distinct non‐oncogenic addictions found in cancer cells, including synthetic lethal interactions, the underlying stress phenotypes, and arising therapeutic opportunities.
PENGGUNAAN PHENOXY ETHANOL, SUHU DINGIN, DAN KOMBINASI SUHU DINGIN DENGAN PHENOXY DALAM PEMBIUSAN BANDENG UMPAN
Authors: Tahe Suwardi 
Published: October 2016

Usaha penangkapan tuna dan cakalang semakin berkembang dan penggunaan bandeng umpan hidup kian marak. Kendala dalam penanganan umpan hidup adalah penanganan sewaktu panen dan pengangkutan sampai ke lokasi penangkapan. Untuk itu telah dilakukan pengangkutan bandeng umpan dengan phenoxy ethanol (200 mg/L), suhu dingin (23°C), dan kombinasi suhu dingin dengan phenoxy ethanol dalam berbagai kepadatan yaitu 5, 10, dan 15 ekor/5L yang dirancang dengan acak lengkap pola faktorial masing-masing tiga ulangan. Hasil penelitian menunjukkan bahwa jumlah kepadatan yang baik adalah berkisar 5--10 ekor/5L dan pengangkutan dengan suhu dingin dan kombinasi suhu dingin dan phenoxy ethanol dapat diaplikasikan oleh pengguna, karena sintasan didapat antara 90%--100% dengan durasi sedatif 6 jam.
APLIKASI REKAYASA GENETIK PADA BUDIDAYA IKAN DI INDONESIA
Authors: Arifin Otong Zenal , Ath-thar Muhammad Hunaina Fariddudin , Gustiano Rudhy 
Published: October 2016

Perbaikan mutu genetik ikan merupakan suatu langkah lanjut dari kegiatan budidaya ikan yang bertujuan untuk meningkatkan produksi dan produktivitas suatu komoditas apabila teknologi budidaya sudah optimal diterapkan. Strategi perbaikan mutu genetik dapat dilakukan baik secara konvensional melalui seleksi dan hibridisasi atau molekuler melalui rekayasa genetik. Di Indonesia, upaya perbaikan mutu genetik melalui rekayasa genetik telah mulai dilakukan sejak tahun 1980. Rekayasa genetik yang pertama kali diterapkan adalah manipulasi set kromosom untuk menghasilkan klon ginogen yang dapat mempercepat pemurnian galur dan poliploidi untuk menghasilkan populasi ikan yang memiliki set kromosom lebih dari 2N. Aplikasi rekayasa genetik yang kedua adalah manipulasi kelamin untuk memanfaatkan potensi “sexual dimorphisms” pada pertumbuhan ikan konsumsi dan sifat eksotis pada ikan hias. Aplikasi rekayasa genetik yang ketiga adalah transgenik atau DNA rekombinan. Teknologi, aplikasi, perkembangan, potensi, dan manfaat rekayasa genetik di atas bagi perkembangan budidaya ikan di Indonesia diuraikan dalam makalah ini.
Outcomes Comparison of In-Vitro Fertilization (IVF) between GnRH Agonist Trigger and hCG for Final Oocyte Maturation
Authors: Permana Ade 
Published: October 2016

<p><strong>Introduction:</strong> For decades, human chorionic gonadotropin (hCG) has been used for final oocyte maturation. It is proven to have highest life birth rate, comparing to gonadotropin releasing hormone (GnRH) agonist, but unfortunately it can increases the risk of developing ovarian hyper stimulation syndrome (OHSS). There is an ongoing debate over the optimal agent that can trigger final oocyte maturation in antagonist protocol, leading to higher IVF success rate without increasing the risk of OHSS.</p><p><strong>Objective:</strong> To compare IVF outcomes in patient using GnRH agonist trigger and hCG for final oocyte maturation.</p><p><strong>Method: </strong>A retrospective review of in vitro fertilization patient at Daya Medika Clinic and Yasmin Clinic.</p><p><strong>Result: </strong>76 women were analyzed consist of 38 women using GnRH agonist and 38 women using hCG. We found no significant differences on biochemical pregnancy rate (24.00% and 20.51%) between two group, but there were significant differences on fertilization rate (67.72% and 61.32%), cleavage rate (95.04% and 88.92%) and blastocyst rate (13.90% and 7.38%). There was one patient developed OHSS in hCG group</p><p><strong>Conclusion: </strong>GnRH agonists can be considered for use as a trigger final oocyte maturation in the antagonist protocol because some IVF outcomes data showed that GnRH agonists triggering were better than hCG without increasing the risk of OHSS</p>
Comparative Study Between Mock Embryo Transfer Prior To The Treatment Cycle and Real Embryo Transfer In In Vitro Fertilization
Authors: Lubis Hilma Putri 
Published: October 2016

<p><strong>Introduction</strong><strong></strong></p><p>A trial or mock embryo transfer (ET) may influence pregnancy rates and it performed prior to ET allows the clinician to assess the uterine cavity and the utero-cervical angle. The aim of this study is to compare the consistency of the type of ET in mock ET with real ET.</p><p><strong>Material &amp; Methods</strong></p><p>A retrospective comparative analysis of patients who underwent in vitro fertilization or ICSI cycle from January 2014 to December 2014 in Halim Fertility Center was done. The type of transfer was divided into two groups: ‘easy’ or ‘difficult’. An easy ET was defined as a transfer that occurred without the use of manipulation or other instrumentation and difficult ET was considered when additional instrumentation was required.</p><p><strong>Results</strong></p><p>From the study, 103 patients who underwent Mock-ET, we found 58 patients (56.3%) with easy ET and 45 patients (43.7%) with difficult ET, which with hard catheter ET in 17 patients (16.5%), with osfander assistance in 20 patients (19.4%) and with stylet in 8 patients (7,8%). 58 patients with Easy Mock ET group were entirely easy real ET (100%) and 45 patients with difficult Mock ET group also entirely were difficult real ET (100%). The Statistical analysis shows no significant difference between the mock ET and real ET groups (p&gt;0,05). In easy real ET, clinical pregnancy rates were 32.8% and in difficult real ET, clinical pregnancy rates were 26.7% with no significant difference between the groups (p&gt;0,05).</p><p><strong>Conclusion:</strong></p><p>Mock ET prior to the treatment cycle is consistent with real ET.</p>
Lost to Follow-up Among Patients who Underwent Vasectomy reversal with Double Layer Microscopic Techniques in 2011-2015
Authors: Birowo Ponco 
Published: October 2016

<p>Vasectomy already been accepted by the society as easy and effective method for male contraceptive. However, some patients want to restore their fertility status due to divorce or re-marriage cases. Techniques in performing vasectomy reversal are varying with their own advantages and disadvantages. One of the techniques is double layer microscopy vasectomy reversal. We evaluate the success rate of this technique based on the semen analysis. The success rate was good with around 98.5% patients with complete follow up had sperm in their semen analysis<a href="file:///C:/Users/Mohamad%20Mostafa/Desktop/Knowledge%20E/In%20Press%20Conferences/ASPIRE-2016/Source-Manuscripts/v2-%20Revised/21.%20Lost%20to%20Follow-up%20Among%20Patients%20who%20Underwent%20Vasectomy%20%20PB%2004082016.docx#_msocom_1">[MP1]</a> . However, total success of follow up were very low (5 out of 19 patients) even though the cost of reversal vasectomy was quite expensive (around 3.000$USD). Lost to follow up rate of reversal vasectomy was 26%.</p>
Acetabular Fractures in the Elderly
Authors: Manson Ted , Schmidt Andrew H. 
Published: October 2016

➢ In the physiologically compromised elderly patient with an acetabular fracture, nonoperative treatment is associated with functional outcomes (as indicated by the Western Ontario and McMaster Universities Osteoarthritis Index [WOMAC] scores) similar to those seen in healthier patients who undergo open reduction and internal fixation, with similar mortality and lesser need for late conversion to total hip arthroplasty.➢ Open reduction and internal fixation of displaced acetabular fractures in patients older than 60 years of age is an excellent option provided that the patient does not have risk factors for failure such as acetabular dome (roof) impaction, femoral-head impaction, or a posterior-wall component. Specific techniques to treat quadrilateral plate involvement and dome impaction are necessary to ensure a durable result. More limited operative approaches and percutaneous fixation have a role in this patient population to minimize the morbidity associated with more extensive exposures.➢ The clinical and radiographic outcome of posterior-wall acetabular fractures that have associated comminution, marginal impaction, and/or femoral-head impaction fractures is predictably poor, with rapid onset of posttraumatic arthritis. Immediate total hip arthroplasty in this population is simple and has outcomes equivalent to those of total hip arthroplasty for coxarthrosis. Total hip arthroplasty should be considered for patients who are ≥60 years of age and have posterior-wall acetabular fractures and perhaps even in younger patients when there are multiple injury factors that predict a poor outcome.➢ The published clinical results of the use of acetabular reconstruction rings, bone graft, and revision arthroplasty techniques appear to be similar to the results of combined internal fixation and insertion of uncemented acetabular components. Surgeons should choose an operative plan that is appropriate to their particular training and skills, the patient’s particular fracture, and hospital resources.
Methane-Hydrate-Formation Processes in Methane/Water Bubbly Flows
Authors: Shimizu T.. , Yamamoto Y.. , Tenma N.. 
Published: October 2016

Summary
Offshore natural-gas production from methane-hydrate (MH) reservoirs has received considerable attention. In this study, the offshore production method is briefly described, followed by the flow loop experiments performed to investigate the formation processes of MH in methane-in-water bubbly flows. Transient processes of phase transformation are characterized by phase paths, flow morphologies, pump heads, and in-situ particle-size measurements. It is realized that an MH slurry is generated by MH shells covering unconverted bubbles, whereas it can be transformed into a colloidal flow with fine crystalline particles under an intense turbulent shear. This study suggests that, in practice, the flow pattern under MH formation would be determined by the phase path and the flow velocity in the pipeline, which is one of the important factors considered in the evaluation of flow-assurance risks.
A New Approach of Gas Lift Wells Production Optimization on Offshore Fields
Authors: Lubnin A. A. , Yudin E. V. , Fazlytdinov R. F. , Khabibullin R. A. , Grishchenko E. N. , Bovt A. V. 
Published: October 2016

Abstract
This article deals with developing production mode of gas lift operation of production wells. A method for solving the issues associated with immediate assessment of the potential flow rate of a large number of wells, as well as for the search of candidates for wellwork is presented. A method for solving optimization problems that arise in operation of gas lift wells on offshore is proposed. Examples of using the proposed method at offshore oil fields of the Socialist Republic of Vietnam that are operated by JV Vietsovpetro are shown.
Selection of Thermo-Chemical EOR for a Large Carbonate Reservoir with Heavy Oil Based on Results of Laboratory Experiments
Authors: Taraskin Evgenii , Ursegov Stanislav 
Published: October 2016

Abstract
This work summarizes the results obtained during the laboratory tests carried out to choose the most efficient thermo-chemical EOR for the further development of the Permian - Carboniferous reservoir of the Usinsk field which is the largest object with heavy oil (its viscosity is around 710 mPa*s) in the Timan-Pechora oil and gas region located in the North European part of Russia. The laboratory experiments were conducted using the stacked models of full-sized and standard-sized core samples of carbonate rocks of the reservoir. Due to the oil-wet characteristic of the carbonate formations, the large volume model was initially imbibed by oil without modeling the water connate saturation. The results of these studies suggest that the combined use of hot water with a temperature of 250°C and a NOP surfactant increases the oil recovery factor up to 38 %. The reason of this relatively low efficiency of the considered thermo-chemical EOR is connected to saving the hydrophobic characteristic of the carbonate formations even upon their heating above 200°C.
An Integrated Approach to the Effective Completion of Horizontal Wells with Multistage Fracturing Based on Petrophysical Analysis, Geomechanical Modeling and Reservoir Simulation. Examples from Western Siberia.
Authors: Kovaleva E. , Vishnivetskiy A. , Naumov Yu. , Lavrenkova N. , Ahmedsafin S. , Rybalchenko V. , Varyagov S. , Kreknin S. , Borovinskiy Yu. 
Published: October 2016

Abstract
This paper describes the integrated approach to decision making on the optimized horizontal well completion with multistage fracturing (MSF) based on petrophysical, geomechanical and numerical modeling. This approach is pertinent in case of insufficient target exploration including absence of a full scale geo&hydromodel (FSGHM). The basic work process of the proposed approach is based on consistent efforts of a petrophysicist, geomechanical engineer, fracturing engineer and reservoir engineer and thus includes petrophysical and geomechanical modeling, fracturing design, numerical modeling and final planned well flow rate evaluation. The role of geomechanics in the production chain is to determine zones with the lowest and the highest fracture gradients to control fracture location and analyze fracture geometry. The basic objective of the proposed approach is to develop a set of recommendations to select: Optimized frac sleeve spacing to increase production during a certain period of well production;Optimized proppant injection volume in general and for each fracture;Recommended horizontal well paths for efficient multistage fracturing completion and reduction of the most probable risks;Frac sleeve placement with provision for the horizontal well path through the section (vertically) to optimize fracture initiation points and maximize reservoir coverage.
Besides, according to the base history, costs of new target development using available production technologies can be estimated based on the completed work package.
An additional objective of this approach is identification of interdependent parameters for further facilitation of downhole surveys and laboratory studies and excluding of non-informative methods.
This paper gives an example of the developed method application for a prospecting and appraisal well at one of the West Siberian fields with a target characterized by extremely low permeability and uncertainty.