bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA‐4317 suppresses human gastric cancer cell proliferation by targeting ZNF322
Authors: Hu Xiaoyi , Zhang Min , Miao Jiyu , Wang Xiaofei , Huang Chen 
Published: September 2017

AbstractStudies have shown that miR‐4317 is dysregulated in tumor, but the biologic role of miR‐4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR‐4317 in human gastric cancer. Quantitative real‐time PCR was used to quantify miR‐4317 expression levels in clinical gastric cancer specimens and cell lines. MTT, colony formation and cell cycle assays were performed to identify the contributions of miR‐4317 to cell proliferation in gastric cancer cell lines. The results showed that miR‐4317 was significantly decreased in 17 clinical gastric cancer specimens compared with adjacent non‐tumor stomach tissues. Forced expression of miR‐4317 suppressed gastric cancer cell proliferation and blocked S‐G2/M transition. Bioinformatics and dual‐luciferase reporter assays confirmed that ZNF322 is a direct target of miR‐4317. Silencing ZNF322 recapitulated the cellular and molecular effects seen upon miR‐4317 overexpression. These findings indicate that miR‐4317 represses the proliferation of gastric cancer cell, at least in part, by targeting and suppressing ZNF322 and that it may serve as a therapeutic target for gastric cancer treatment.
Intra‐ versus Intermolecular Hydrogen Bonding: Solvent‐Dependent Conformational Preferences of a Common Supramolecular Binding Motif from 1H NMR and Vibrational Circular Dichroism Spectra
Authors: Demarque Daniel P. , Merten Christian 
Published: September 2017

AbstractWhen predicting binding properties of small molecules or larger supramolecular aggregates, intra‐ and intermolecular hydrogen bonds are often considered the most important factor. Spectroscopic techniques such as 1H NMR spectroscopy are typically utilized to characterize such binding events, but interpretation is often qualitative and follows chemical intuition. In this study, we compare the effects of intramolecular hydrogen bonding and solvation on two chiral 2,6‐pyridinediyl‐dialkylamides. In comparison with 1H NMR spectroscopy, vibrational circular dichroism (VCD) spectroscopy proved to be more sensitive to conformational changes. In fact, the change of the solvent from CDCl3 to [D6]DMSO generates mirror‐image VCD spectra for the same enantiomer. Here, the common sense that the sterically less hindered group is more prone to solvation proved to be wrong according predicted VCD spectra, which clearly show that both asymmetric amide hydrogens are equally likely to be solvated, but never simultaneously. The competition between intra‐ and intermolecular hydrogen bonding and their importance for a correct prediction of spectral properties are discussed.
Heterogeneous impact of smoking on major salivary gland cancer according to histopathological subtype: A case‐control study
Authors: Sawabe Michi , Ito Hidemi , Takahara Taishi , Oze Isao , Kawakita Daisuke , Yatabe Yasushi , Hasegawa Yasuhisa , Murakami Shingo , Matsuo Keitaro 
Published: September 2017

BACKGROUNDMajor salivary gland cancers (M‐SGCs) are rare, and have distinct heterogeneous histopathological subtypes. To the authors' knowledge, no consistent evidence of an association between cigarette smoking and the risk of M‐SGCs has appeared to date. Furthermore, evidence of potential heterogeneity in the impact of smoking on histopathological subtypes is scarce, despite the fact that the histopathological subtypes of M‐SGC exhibit different genetic features.METHODSThe authors conducted a case‐control study to investigate the association between smoking and M‐SGC by histopathological subtype. Cases were 81 patients with M‐SGCs and the controls were 810 age‐matched and sex‐matched first‐visit outpatients without cancer treated at Aichi Cancer Center Hospital from 1988 to 2005. Odds ratios (OR) and 95% confidence intervals (95% CI) were assessed by conditional logistic regression analysis with adjustment for potential confounders.RESULTSSmoking was found to be associated with a significantly increased risk of M‐SGC overall, with an OR of 3.45 (95% CI, 1.58‐7.51; P =.001) for heavy smokers compared with never‐smokers. A significant dose‐response relationship was observed (P for trend, .001). When stratified by histological subtype, no obvious impact of smoking was observed among patients with mucoepidermoid carcinoma (MEC). In contrast, smoking demonstrated a significantly increased risk of M‐SGCs other than MEC, with an OR of 5.15 (95% CI, 2.06‐12.87; P<.001) for heavy smokers compared with never‐smokers. The authors observed possible heterogeneity with regard to the impact of smoking on risk between MEC and M‐SGCs other than MEC (P for heterogeneity, .052).CONCLUSIONSThe results of the current study demonstrate a significant positive association between cigarette smoking and the risk of M‐SGC overall. However, the impact of smoking appeared to be limited to M‐SGCs other than MEC. Cancer 2018;124:118‐24. © 2017 American Cancer Society.
Dynamic searchable encryption with privacy protection for cloud computing
Authors: Ocansey Selasi Kwame , Ametepe Wolali , Li Xiao Wei , Wang Changda 
Published: September 2017

SummaryThe dynamic searchable encryption schemes generate search tokens for the encrypted data on a cloud server periodically or on a demand. With such search tokens, a user can query the encrypted data whiles preserving the data's privacy; ie, the cloud server can retrieve the query results to the user but do not know the content of the encrypted data. A framework DSSE with Forward Privacy (dynamic symmetric searchable encryption [DSSE] with forward privacy), which consists of Internet of Things and Cloud storage, with the attributes of the searchable encryption and the privacy preserving are proposed. Compared with the known DSSE schemes, our approach supports the multiusers query. Furthermore, our approach successfully patched most of the security flaws related to the sensitive information's leakage in the DSSE schemes. Both security analysis and simulations show that our approach outperforms other DSSE schemes with respect to both effectiveness and efficiency.
Cholesterol‐imprinted macroporous monoliths: Preparation and characterization
Authors: Stepanova Mariia А. , Kinziabulatova Lilia R. , Nikitina Anna A. , Korzhikova‐Vlakh Evgenia G. , Tennikova Tatiana B. 
Published: September 2017

AbstractThe development of sorbents for selective binding of cholesterol, which is a risk factor for cardiovascular disease, has a great importance for analytical science and medicine. In this work, two series of macroporous cholesterol‐imprinted monolithic sorbents differing in the composition of functional monomers (methacrylic acid, butyl methacrylate, 2‐hydroxyethyl methacrylate and ethylene dimethacrylate), amount of a template (4, 6 and 8 mol%) used for molecular imprinting, as well as mean pore size were synthesized by in situ free‐radical process in stainless steel housing of 50 mm × 4.6 mm i.d. All prepared materials were characterized regarding to their hydrodynamic permeability and porous properties, as well as examined by BET and SEM methods. Imprinting factors, apparent dynamic dissociation constants, the maximum binding capacity, the number of theoretical plates and the height equivalent to a theoretical palate of MIP monoliths at different mobile phase flow rates were determined. The separation of a mixture of structural analogues, namely, cholesterol and prednisolone, was demonstrated. Additionally, the possibility of using the developed monoliths for cholesterol solid‐phase extraction from simulated biological solution was shown.
Transport of suspended sediments under the influence of bank macro‐roughness
Authors: Juez Carmelo , Bühlmann Iria , Maechler Gaetan , Schleiss Anton J. , Franca Mário J. 
Published: September 2017

AbstractRiver restoration works often include measures to promote morphological diversity and enhance habitat suitability. One of these measures is the creation of macro‐roughness elements, such as lateral cavities and embayments, in the banks of channelized rivers. However, in flows that are heavily charged with fine sediments in suspension, such as glacier‐fed streams and very low‐gradient reaches of large catchment rivers, these lateral cavities may trap these sediments. Consequently, the morphological changes may be affected, and the functionality of the restoration interventions may be compromised. Herein, we analyse the influence of these macro‐roughness elements on the transport of fine sediments in the main channel. Laboratory tests with uniform flow charged with sediments in a channel with banks equipped with large‐scale rectangular roughness elements were carried out. The laboratory experiments covered a wide range of rectangular cavity geometrical configurations and shallowness ratios. The influence of key parameters such as flow shallowness, geometric ratios of the cavities and initial sediment concentration was tested. Surface particle image velocimetry, sediment samples and temporal turbidity records were collected during the experiments. The amount of sediments captured by the cavities, the temporal evolution of the concentration of sediments in suspension and the flow hydrodynamics are cross‐analysed and discussed. It is shown that the trapping efficiency of the macro‐roughness elements is a clear function of the channel geometry and the shallowness of the flow. Copyright © 2017 John Wiley & Sons, Ltd.
A one‐dimensional process‐based approach to study reservoir sediment dynamics during management operations
Authors: Guertault Lucie , Camenen Benoit , Paquier André , Peteuil Christophe 
Published: September 2017

AbstractA comprehensive understanding of the dynamics of erosion and sedimentation in reservoirs under different management conditions is required to anticipate sedimentation issues and implement effective sediment management strategies. This paper describes a unique approach combining fluvial geomorphology tools and morphodynamic modeling for analyzing the sediment dynamics of an elongated hydropower reservoir subjected to management operations: the Génissiat Reservoir on the Rhône River. Functional sub‐reaches representative of the reservoir morphodynamics were delineated by adapting natural river segmentation methods to elongated reservoirs. The segmentation revealed the link between the spatial and temporal reservoir changes and the variability of longitudinal flow conditions during reservoir management operations. An innovative modeling strategy, incorporating the reservoir segmentation into two sediment transport codes, was implemented to simulate the dynamics of erosion and sedimentation at the reach scale during historic events. One code used a bedload approach, based on the Exner equation with a transport capacity formula, and the other used a suspended load approach based on the advection–dispersion equation. This strategy provided a fair quantification of the dynamics of erosion and sedimentation at the reach scale during different management operations. This study showed that the reservoir morphodynamics is controlled by bedload transport in upper reaches, graded suspended load transport of sand in middle reaches and suspended load transport of fine sediments in lower reaches. Eventually, it allowed a better understanding of the impact of dam management on sediment dynamics. Copyright © 2017 John Wiley & Sons, Ltd.
Geochemical characteristics, depositional environment, and controlling factors of Lower Cretaceous shales in Chagan Sag, Yingen‐Ejinaqi Basin
Authors: Niu Zicheng , Liu Guangdi , Cao Zhe , Guo Dianbin , Wang Peng , Tang Guo 
Published: September 2017

The Lower Cretaceous shales of Chagan Sag, Yingen‐Ejinaqi Basin, are considered as the source rocks of hydrocarbons discovered in the sag. Borehole shales from the Lower Cretaceous were subjected to comprehensive geochemical study to evaluate the quality of source rock, to reconstruct the paleodepositional environment and to determine the controlling factors of organic matter enrichment. Content of present‐day total organic carbon, and hydrogen index, averaging 1.4% and 259.2 mg hydrocarbon/g total organic carbon, respectively reveal poor to fair quality of organic matter. Average value of vitrinite reflectance (Ro) of 1.0% indicates mature organic matter. Maceral composition and biomarker analysis provide evidence for a mixed aquatic and terrigeous organic matter input. Based on Sr/Cu, Sr/Ba, and gammacerane index, we propose that organic matter was deposited in closed hypersaline under hot and arid climate conditions. The ratios of V/(V + Ni), and pristane/phytane (Pr/Ph), as well as the presence of β‐carotene suggest a stratified water column with anoxic bottom water condition. Low to moderate productivity as a result of deficient nutrient supply, the excellent organic matter preservation condition under stratified anoxic water column and high sedimentary rate controlled the quality of source rock. In addition, influence of factors such as content of clay minerals on organic matter enrichment cannot be ignored.
Structural connectivity‐defined thalamic subregions have different functional connectivity abnormalities in multiple sclerosis patients: Implications for clinical correlations
Authors: d'Ambrosio Alessandro , Hidalgo de la Cruz Milagros , Valsasina Paola , Pagani Elisabetta , Colombo Bruno , Rodegher Mariaemma , Comi Giancarlo , Filippi Massimo , Rocca Maria A. 
Published: September 2017

AbstractIn spite of the well‐known importance of thalami in multiple sclerosis (MS), only limited data on whole and subregional thalamic functional connectivity (FC) changes are available. Using diffusion tensor imaging, we performed a structural connectivity based thalamic parcellation and investigated subregional thalamic resting‐state (RS) FC alterations and their relationship with clinical/cognitive measures in MS. MRI data from a reference set of healthy controls (HC) were used to parcellate the thalami into five subregions, according to their structural connectivity. For each thalamic subregion, a seed‐based RS FC analysis was performed in 187 MS patients and 94 HC. Correlations between thalamic RS FC and clinical/cognitive variables were assessed. Compared to HC, MS patients showed increased intra‐ and inter‐thalamic RS FC for almost all thalamic subregions, and increased RS FC between all thalamic subregions and the left insula. Frontal and motor thalamic subregions also showed reduced RS FC with the caudate nucleus. For the temporal thalamic subregion, we observed reduced RS FC with the ipsilateral thalamus, anterior and middle cingulate cortex, and cerebellum. Compared to cognitively preserved, cognitively impaired MS patients had higher thalamic RS FC with several temporal areas. In MS patients, lower RS FC between thalamic subregions and the caudate and cingulate cortex correlated with worse motor performance, whereas higher RS FC with the insula correlated with better motor performance. The main thalamic subregions have different RS‐FC abnormalities in MS patients. Increased thalamic RS FC with the insula may have a compensatory role, whereas increased RS FC with temporal areas, observed in patients with cognitive impairment may reflect maladaptive mechanisms. Hum Brain Mapp 38:6005–6018, 2017. © 2017 Wiley Periodicals, Inc.
Quality specification and status of internal quality control of cardiac biomarkers in China from 2011 to 2016
Authors: Li Tingting , Wang Wei , Zhao Haijian , He Falin , Zhong Kun , Yuan Shuai , Wang Zhiguo 
Published: September 2017

BackgroundThis study aimed to investigate the status of internal quality control (IQC) for cardiac biomarkers from 2011 to 2016 so that we can have overall knowledge of the precision level of measurements in China and set appropriate precision specifications.MethodsInternal quality control data of cardiac biomarkers, including creatinine kinase MB (CK‐MB) (μg/L), CK‐MB(U/L), myoglobin (Mb), cardiac troponin I (cTnI), cardiac troponin T (cTnT), and homocysteines (HCY), were collected by a web‐based external quality assessment (EQA) system. Percentages of laboratories meeting five precision quality specifications for current coefficient of variations (CVs) were calculated. Then, appropriate precision specifications were chosen for these six analytes. Finally, the CVs and IQC practice were further analyzed with different grouping methods.ResultsThe current CVs remained nearly constant for 6 years. cTnT had the highest pass rates every year against five specifications, whereas HCY had the lowest pass rates. Overall, most analytes had a satisfactory performance (pass rates >80%), except for HCY, if one‐third TEa or the minimum specification were employed. When the optimal specification was applied, the performance of most analytes was frustrating (pass rates < 60%) except for cTnT. The appropriate precision specifications of Mb, cTnI, cTnT and HCY were set as current CVs less than 9.20%, 9.90%, 7.50%, 10.54%, 7.63%, and 6.67%, respectively. The data of IQC practices indicated wide variation and substantial progress.ConclusionThe precision performance of cTnT was already satisfying, while the other five analytes, especially HCY, were still frustrating; thus, ongoing investigation and continuous improvement for IQC are still needed.
Comparison of vessel enhancement algorithms applied to time‐of‐flight MRA images for cerebrovascular segmentation
Authors: Phellan Renzo , Forkert Nils D. 
Published: September 2017

PurposeVessel enhancement algorithms are often used as a preprocessing step for vessel segmentation in medical images to improve the overall segmentation accuracy. Each algorithm uses different characteristics to enhance vessels, such that the most suitable algorithm may vary for different applications. This paper presents a comparative analysis of the accuracy gains in vessel segmentation generated by the use of nine vessel enhancement algorithms: Multiscale vesselness using the formulas described by Erdt (MSE), Frangi (MSF), and Sato (MSS), optimally oriented flux (OOF), ranking orientations responses path operator (RORPO), the regularized Perona–Malik approach (RPM), vessel enhanced diffusion (VED), hybrid diffusion with continuous switch (HDCS), and the white top hat algorithm (WTH).MethodsThe filters were evaluated and compared based on time‐of‐flight MRA datasets and corresponding manual segmentations from 5 healthy subjects and 10 patients with an arteriovenous malformation. Additionally, five synthetic angiographic datasets with corresponding ground truth segmentation were generated with three different noise levels (low, medium, and high) and also used for comparison. The parameters for each algorithm and subsequent segmentation were optimized using leave‐one‐out cross evaluation. The Dice coefficient, Matthews correlation coefficient, area under the ROC curve, number of connected components, and true positives were used for comparison.ResultsThe results of this study suggest that vessel enhancement algorithms do not always lead to more accurate segmentation results compared to segmenting nonenhanced images directly. Multiscale vesselness algorithms, such as MSE, MSF, and MSS proved to be robust to noise, while diffusion‐based filters, such as RPM, VED, and HDCS ranked in the top of the list in scenarios with medium or no noise. Filters that assume tubular‐shapes, such as MSE, MSF, MSS, OOF, RORPO, and VED show a decrease in accuracy when considering patients with an AVM, because vessels may vary from its tubular‐shape in this case.ConclusionsVessel enhancement algorithms can help to improve the accuracy of the segmentation of the vascular system. However, their contribution to accuracy has to be evaluated as it depends on the specific applications, and in some cases it can lead to a reduction of the overall accuracy. No specific filter was suitable for all tested scenarios.
National trends in long‐term use of prescription opioids
Authors: Mojtabai Ramin 
Published: September 2017

AbstractPurposeThis study examined recent trends and correlates of prescription opioid use and long‐term use in the United States.MethodsData were from 47 356 adult participants of National Health and Nutrition Survey from 1999‐2000 to 2013‐2014. Participants were asked about prescription medications used in the past 30 days. Long‐term use of prescription opioids was defined by use for 90 days or longer.ResultsThe prevalence of prescription opioid use increased from 4.1% of US adults in 1999‐2000 to 6.8% in 2013‐2014 (odds ratio [OR] = 1.38, 95% confidence interval [CI] = 1.10‐1.75, P = .007). This trend was driven by a sharp increase in long‐term use which increased from 1.8% to 5.4% (OR = 2.22, 95% CI = 1.65‐3.00, P < .001). Of all opioid users in 2013‐2014, 79.4% were long‐term users compared with 45.1% in 1999‐2000. Long‐term use was associated with poorer physical health, concurrent benzodiazepine use, and history of heroin use.ConclusionsThe findings highlight the need for research on potential benefits and harms of long‐term use of opioids and efforts to restrict long‐term use to patients for whom the benefits outweigh the risks.
Poor recovery from cystic fibrosis pulmonary exacerbations is associated with poor long‐term outcomes
Authors: Sanders Don B. , Zhao Qianqian , Li Zhanhai , Farrell Philip M. 
Published: September 2017

AbstractRationalePeople with CF treated with IV antibiotics for a pulmonary exacerbation (PEx) frequently fail to recover to baseline FEV1. The long‐term impact of these events has not been studied.ObjectivesTo determine if a patient's spirometric recovery after a PEx is associated with time to next PEx within 1 year, the spirometric recovery after the next PEx, and/or the number of PEx episodes in the next 3 years.MethodsWe used data from the CF Foundation Patient Registry from 2004 to 2011. We randomly selected one PEx per patient that met inclusion/exclusion criteria. Patients were defined as Non‐Responders if their best FEV1 (in liters) recorded in the 3 months after the PEx was <90% of the best FEV1 (in liters) in the 6 months before the PEx. We compared Responders and Non‐Responders using multivariable regression models.ResultsWe randomly chose 13 954 PEx episodes that met inclusion/exclusion criteria. A total of 2 762 (19.8%) patients were classified as Non‐Responders. Non‐Responders had a shorter median time to the next PEx, 235 (95%CI 218, 252) days, versus >365 days for Responders. Thirty‐four percent of Non‐Responders at the initial PEx were also Non‐Reponders at the next PEx, versus 20% of Responders at the initial PEx. Non‐Responders had more PEx episodes over the next 3 years, 4.99 (95%CI 4.84, 5.13), than Responders, 3.46 (95%CI 3.41, 3.51).ConclusionsPoor recovery after a PEx is associated with a shorter time to the next PEx, increased risk of poor recovery at a second PEx, and more frequent subsequent PEx treatments.