bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eyeing DNA barcoding for species identification of fish larvae
Authors: Chan Wan Wen Rochelle , Chang Jia Jin Marc , Tan Charles Zhiming , Ng Jie Xin , Ng Matthew Hui‐Chieh , Jaafar Zeehan , Huang Danwei 
Published: September 2024

AbstractIdentification of fish larvae based on morphology is typically limited to higher taxonomic ranks (e.g., family or order), as larvae possess few morphological diagnostic characters for precise discrimination to species. When many samples are presented at any one time, the use of morphology to identify such specimens can be laborious and time‐consuming. Using a reverse workflow for specimen sorting and identification leveraging high‐throughput DNA sequencing, thousands of fish larvae can be DNA barcoded and sorted into molecular operational taxonomic units (mOTUs) in a single sequencing run with the nanopore sequencing technology (e.g., MinION). This process reduces the time and financial costs of morphology‐based sorting and instead deploys experienced taxonomists for species taxonomic work where they are needed most. In this study, a total of 3022 fish larval specimens from plankton tows across four sites in Singapore were collected and sorted based on this workflow. Eye tissue from individual samples was used for DNA extraction and sequencing of cytochrome c oxidase subunit I. We generated a total of 2746 barcodes after quality filtering (90.9% barcoding success), identified 2067 DNA barcodes (75.3% identification success), and delimited 256 mOTUs (146 genera, 52 families). Our analyses identified specific challenges to species assignment, such as the potential misidentification of publicly available sequences used as reference barcodes. We highlighted how the conservative application and comparison of a local sequence database can help resolve identification conflicts. Overall, this proposed approach enables and expedites taxonomic identification of fish larvae, contributing to the enhancement of reference barcode databases and potentially better understanding of fish connectivity.
Clinical guidance for cannabidiol‐associated hepatotoxicity: A narrative review
Authors: Eadie Lauren , Lo Lindsay A. , Boivin Michael , Deol Jagpaul K. , MacCallum Caroline A. 
Published: September 2024

AbstractThere is increasing evidence that cannabidiol (CBD) use is associated with clinically significant liver enzyme (LE) elevations and drug‐induced liver injury (DILI). The proportion of LE elevations and DILI events reported in the literature meet the Council for International Organizations of Medical Sciences' (CIOMS) classification of a common adverse drug reaction. However, these potential adverse events are unknown to many clinicians and may be overlooked. The increasing use of CBD for both medical and non‐medical use necessitates clear direction in the diagnosis and management of CBD‐associated hepatotoxicity. To our knowledge, no such clinical guidance currently exists. For people presenting with elevated LEs, CBD use should be screened for and be considered in the differential diagnosis. This narrative review will provide clinicians with guidance in the prevention, detection, and management of CBD‐related hepatotoxicity.
Protection against influenza hospitalizations from enhanced influenza vaccines among older adults: A systematic review and network meta‐analysis
Authors: Ferdinands J. M. , Blanton L. H. , Alyanak E. , Chung J. R. , Trujillo L. , Taliano J. , Morgan R. L. , Fry A. M. , Grohskopf L. A. 
Published: September 2024

AbstractBackgroundInfluenza vaccines are available to help protect persons aged ≥65 years, who experience thousands of influenza hospitalizations annually. Because some influenza vaccines may work better than others, we sought to assess benefit of high‐dose (HD), adjuvanted (ADJ), and recombinant (RIV) influenza vaccines (“enhanced influenza vaccines”) compared with standard‐dose unadjuvanted influenza vaccines (SD) and with one another for prevention of influenza‐associated hospitalizations among persons aged ≥65 years.MethodsWe searched MEDLINE, Embase, CINAHL, Scopus, and Cochrane Library to identify randomized or observational studies published between January 1990 and October 2023 and reporting relative vaccine effectiveness (rVE) of HD, ADJ, or RIV for prevention of influenza‐associated hospitalizations among adults aged ≥65 years. We extracted study data, assessed risk of bias, and conducted random‐effects network meta‐analysis and meta‐regression.ResultsWe identified 32 studies with 90 rVE estimates from five randomized and 27 observational studies (71,459,918 vaccinated participants). rVE estimates varied across studies and influenza seasons. Pooled rVE from randomized studies was 20% (95% CI −54 to 59) and 25% (95% CI −19 to 53) for ADJ and HD compared with SD, respectively; rVE was 6% (95% CI −109 to 58) for HD compared with ADJ; these differences were not statistically significant. In observational studies, ADJ, HD, and RIV conferred modestly increased protection compared with SD (rVE ranging from 10% to 19%), with no significant differences between HD, ADJ, and RIV. With enhanced vaccines combined, rVE versus SD was 18% (95% CI 3 to 32) from randomized and 11% (95% CI 8 to 14) from observational evidence. Meta‐regression of observational studies suggested that those requiring laboratory confirmation of influenza reported greater benefit of enhanced vaccines.ConclusionsHD, ADJ, and RIV provided stronger protection than SD against influenza hospitalizations among older adults. No differences in benefit were observed in comparisons of enhanced influenza vaccines with one another.
Platform design and rent extraction*
Authors: Piolatto Amedeo , Schuett Florian 
Published: September 2024

We study the design of online platforms that aggregate information and facilitate transactions. Leading players in the industry (e.g., the Booking Group) hold two types of platforms in their portfolio: revealing platforms that disclose the identity of transaction partners (like Booking.com) and anonymous platforms that do not (like Hotwire.com). Anonymous platforms offer discounts but lead to inefficient matching between consumers and firms. We develop a model in which horizontally differentiated firms sell to heterogeneous consumers both directly and via a platform that enlarges the pool of consumers they can attract. The platform charges firms for transactions it intermediates and can choose to offer an anonymous sales channel in addition to a revealing one. We show that offering both sales channels is profitable not only because it allows the platform to implement price discrimination, as suggested by the literature on opaque selling, but also because it improves rent extraction. The anonymous channel breaks the link between the price on the revealing channel and the firms' outside option; moreover, it can reduce double marginalisation. The welfare impact of the anonymous channel is ambiguous: while it sometimes leads to market expansion, it also causes inefficiently high transport costs.
Fungus gnat pollination in Arisaema urashima: the interplay of lethal traps and mutualistic nurseries
Authors: Suetsugu K. , Nishigaki H. , Sato R. , Kakishima S. , Ishitani E. , Fukushima S. , Sugiura S. , Sueyoshi M. 
Published: September 2024

Abstract
While most flowering plants engage in mutualistic interactions with their pollinators, Arisaema species employ a unique, seemingly antagonistic strategy by imprisoning and causing the pollinators to perish within their spathes. Recent studies have revealed that Arisaema thunbergii primarily relies on a fungus gnat, Leia ishitanii, with some individuals possibly escaping female spathes after oviposition.
We investigated interactions between A. urashima and its pollinating fungus gnats, given that A. urashima is closely related to A. thunbergii. Specifically, we tested whether decaying A. urashima serve as brood‐sites for some pollinators and whether these pollinators can escape seemingly lethal floral traps. We retrieved A. urashima spathes together with adult insect corpses trapped within the spathes and incubated the spathes to see if conspecific insects emerged. In addition, under laboratory conditions, we observed the escape behaviour of Sciophila yokoyamai, whose next‐generation adults most frequently emerge from the decaying spathes.
Our findings indicate that S. yokoyamai almost always escapes from the female spathe after oviposition while using the inflorescence as a nursery. In contrast, other pollinators of A. urashima, including Mycetophila spp., remain trapped and perished within the spathes.
This study demonstrates that A. urashima spathes can function both as lethal traps and mutualistic nurseries, with outcomes differing among pollinator species. Our results also suggest that the contribution of certain pollinators to Arisaema reproduction is underestimated or even neglected, given that information on their pollinator assemblages has been based on floral visitors trapped within the inflorescences.
Effect of grafting tomato onto Solanum torvum on the population dynamics of Meloidogyne incognita and M. javanica and crop yield losses
Authors: Fullana Aïda Magdalena , Expósito Alejandro , Pujolà Montserrat , Achaerandio Isabel , Cunquero Marina , Loza‐Alvarez Pablo , Giné Ariadna , Sorribas Francisco Javier 
Published: September 2024

AbstractMeloidogyne spp. are the most devastating plant‐parasitic nematodes affecting tomato worldwide. Although resistant cultivars and rootstocks are used, selection for virulence occurs in the pathogen. Consequently, using other resistance sources, such as Solanum torvum, could improve resistance durability. Several experiments in microplots and plastic greenhouses were carried out to determine the potential use of S. torvum as a tomato rootstock to protect against M. incognita and M. javanica. In microplots, the relationship between nematode density at transplanting (Pi) and multiplication rate did not differ between Meloidogyne species in either ungrafted or grafted tomato. However, maximum multiplication rate and maximum density on grafted tomato were 1.27% and 2.93% those on ungrafted, respectively. The grafted tomato plants yielded between 2.9 and 7.5 more times than the ungrafted plants at Pi ≥ 100 eggs + J2s per 100 cm3 of soil, but no differences were observed in plastic greenhouse where a large amount of scion‐rooting occurred. In microplots, the quality of the tomato fruits of ungrafted and grafted plants was affected by the Pi. In parallel, some pot experiments were conducted on S. torvum and susceptible eggplant to determine the putative selection for nematode virulence to S. torvum and the nematode fitness cost. These showed that the nematode subpopulations infected and reproduced less on S. torvum than on eggplant. However, the female fertility was only reduced after development of three or four subpopulations on S. torvum. Finally, a histopathological study showed that nematode infection and development in S. torvum was delayed compared to eggplant.
Pathotypes and races of Pseudoperonospora cubensis: Two concepts of virulence differentiation
Authors: Lebeda Aleš , Křístková Eva , Sedláková Božena 
Published: September 2024

AbstractPseudoperonospora cubensis, causal agent of cucurbit downy mildew, is a common and highly variable pathogen of cucurbit vegetables. Pathogen virulence can be considered on two different levels, that is, pathotypes and races. Pathotypes characterize the interactions between pathogen isolates and the set of genotypes represented by different species and/or genera of the host plant family. Races are characterized by the interactions between pathogen isolates and the set of genotypes (e.g., cultivars, lines, accessions) of one host species (or very closely related species). Differential sets for pathotype and/or race determination are composed of distinct (different) genotypes. Until recently, only differentiation of pathotypes was used to characterize P. cubensis. Most recent research of virulence variation in P. cubensis populations in the Czech Republic working with the Cucumis melo differential set demonstrated the existence of a large number of races of this pathogen. The differential capacity of this set was primarily created for the determination and denomination of virulence phenotypes/races of cucurbit powdery mildew (Golovinomyces orontii, Podosphaera xanthii). However, this set is also efficient for differentiating virulence phenotypes in P. cubensis. This means that one set of C. melo differential genotypes can be used for two independent plant–pathogen systems: cucurbit powdery mildew and cucurbit downy mildew. Results showed that isolates of P. cubensis developing identical virulence reaction patterns at the level of pathotype, develop different reaction patterns at the level of races and vice versa. It is demonstrated and explained that pathotypes and races are two different categories for the assessment of virulence variation, which should be clearly defined, recognized and used within international scientific and breeding communities.
The use of air abrasion method to treat caries in autistic patients at the age of 6 years: A non‐randomized controlled trial
Authors: Kuvatbayeva Urnissyam A. , Iskakova Maryam K. 
Published: September 2024

AbstractBackgroundAutism Spectrum Disorders (ASD) encompass a range of conditions characterized by difficulties in social interaction and communication. Managing dental treatment for children with autism presents challenges due to their reluctance to interact with dentists, which can be as high as 65%.ObjectivesThis prospective non‐randomized study, conducted at the Kazakh‐Russian Medical University from January 15, 2023, to June 20, 2023, aimed to assess the efficacy of air abrasion caries treatment (AAbCT) compared to the standard method.InterventionThe intervention involved applying air abrasion (AAb) treatment without anesthesia using aluminum oxide powder in the AAbCT group, while the control group received cavity preparation with local anesthesia.ResultsIn this study, it was noted that the AAb method of tooth preparation was not different in efficacy from the traditional method for a population of children with autism, and could be used without anesthesia. A greater willingness to cooperate was noted (p = .0372).ConclusionThe AAb is an effective and safe treatment for children with ASD. Further studies needed to investigate its efficacy and safety in combination with behavioral interventions.
Daily within‐fluctuations in need frustration and implications for employee recovery and well‐being: A mixed‐methods study
Authors: Olafsen Anja H. , Kujanpää Miika , Bentzen Marte 
Published: September 2024

IntroductionDaily variations in frustration of basic psychological needs (autonomy, competence, and relatedness) have received limited attention. This mixed‐methods study examines such daily variations and their relations to recovery from work and employee well‐being.MethodThe study uses multilevel modeling of repeated measures through daily surveys from a period of 8 working days across 2 consecutive weeks, combined with in‐depth interviews. A sample of 54 Norwegian health‐care workers completed a total of 242 daily surveys, and follow‐up interviews were conducted with 10 participants.ResultsQuantitative results showed that need frustration at work fluctuates from day to day, with competence frustration notably impairing recovery (i.e., lower psychological detachment and relaxation) and increasing ill‐being (i.e., higher exhaustion and negative work affect). Autonomy frustration was related to increased exhaustion and sleep complaints, while relatedness frustration showed no significant relation to recovery, ill‐being, or sleep. Qualitative findings corroborated and expanded on these results, offering deepened insights into how competence and, sometimes, relatedness need frustration hampered the recovery process and sleep.ConclusionThe results of the current study add to the scarce body of literature on daily fluctuations in need frustration at work and its adverse consequences.
Revisiting the seminal studies of attachment formation and reevaluating what it means to become attached
Authors: Freeman Harry 
Published: September 2024

AbstractWhat does it mean to become attached? Three longitudinal studies established the empirical basis for the existing four‐phase model of attachment formation, a model that has remained unmodified and unexamined for over half a century. In this paper, I revisit the research questions, methods, and findings from the seminal studies to reevaluate the current model. The evidence indicates two distinct definitions of attachment onset. In the first two phases of the model, becoming attached is defined by changes in within‐subject behaviors leading to the selection of a discriminated figure. Defined this way, attachment onset is analogous to how bond formation is currently defined in other mammals and how imprinting is understood in birds. In contrast, the third and fourth phases of the model define attachment onset by forming a goal‐corrected relationship. This second definition is human‐centric and relies on secure base behavior as the signature criterion, the same criteria used to classify secure and insecure patterns of attachment. I argue for a narrower definition of attachment by removing goal‐corrected behavior as a criterion and focusing on the normative process of selection. In addition, I integrate contemporary work on pair bonding in humans and other animals to propose new avenues for conceptualizing and studying attachment formation in infancy and beyond in filial and sexual bonds.
Developmental differences in young children's implied use of cognitive resources in their self‐regulation strategies
Authors: Cardwell Gabrielle Sky , Cole Pamela M. , Weaver Brooke , Leadbeater Jenna M. , Lunkenheimer Erika S. , Buss Kristin A. , Gatzke‐Kopp Lisa , Ram Nilam 
Published: September 2024

AbstractThe emergence of self‐regulation during the preschool years is due, in part, to children's development of cognitive resources that can regulate their behavior. However, there is little direct evidence that age influences the extent to which young children's strategies involve such resources. We investigated age differences in the extent that young children's strategies imply cognitive resources. A sample of 154 children (77 girls; M age 45.15 months, range 30–60 months) and mothers from middle‐class families (M income = $89,541) in a small mid‐Atlantic American city (94.2% White). They participated in a 9‐min delayed reward task in which mothers told children they must wait to open a gift; children were reminded every 3 min. The latency, frequency, and average duration of the extent to which children's strategies implied cognitive resources were examined in relation to task time and age. In line with Kopp's framework, results from multilevel models indicated older preschool‐age children engaged strategies implying higher‐level cognitive resources more quickly and frequently, but not longer than younger children. Regardless of age, children engaged cognitive resources more quickly, more often, and longer in the first 3 min of the task than later in the task, suggesting that such engagement was not sustained. The findings are discussed in terms of both the emergence and complexities of regulatory strategy development.
Evaluating Urban Land Resource Carrying Capacity With Geographically Weighted Principal Component Analysis: A Case Study in Wuhan, China
Authors: Lu Binbin , Shi Yilin , Qin Sixian , Yue Peng , Zheng Jianghua , Harris Paul 
Published: September 2024

ABSTRACTWith the rapid urbanization in China, urban land resources gradually become the core of urban development. This study spatially evaluated the urban land resource carrying capacity (LRCC) with a case study of the built‐up area in Wuhan from 2015 to 2020. Following an evaluation index system, five critical LRCC indicators, including population density, GDP per land area, plot ratio, building density, and road network density, were selected by an analytical hierarchical process. The synthesis of indicators, however, is usually challengeable due to homogeneous assumptions of traditional techniques. In this study, we adopted a local technique, geographically weighted principal component analysis, to calculate a comprehensive carrying pressure (CCP) concerning spatially varying contributions of each indicator on their synthesis across different geographic locations. On mapping these spatial outputs of the built‐up area in Wuhan, the highest CCP was found in the central areas, where population size tends to be influential and the dominant variable in 62.69% of subdistricts. Furthermore, increased construction over the 5 years has led to an increased CCP in some of the peripheries of the built‐up area, and 55.22% of subdistricts show rising changes. With the GWPCA technique, this framework works well in evaluating and analyzing urban LRCC from a new local perspective.
Effects of the angle of head‐down tilt on dynamic cerebral autoregulation during combined exposure to cephalad fluid shift and mild hypercapnia
Authors: Kato Tomokazu , Ogawa Yojiro , Iwasaki Ken‐ichi 
Published: September 2024

AbstractAstronauts experience combined exposure to a cephalad fluid shift and mild hypercapnia during space missions, potentially contributing to health problems. Such combined exposure may weaken dynamic cerebral autoregulation. The magnitude of cephalad fluid shift varies between individuals, and dynamic cerebral autoregulation may be affected more by greater cephalad fluid shift during combined exposure. We evaluated the dose‐dependent effects of head‐down tilt (HDT) on dynamic cerebral autoregulation during acute combined exposure to HDT and 3% CO2 inhalation. Twenty healthy participants were randomly exposed to three angles of HDT (−5°HDT+CO2, −15°HDT+CO2 and −30°HDT+CO2). After 15 min of rest, participants inhaled room air for 10 min in a horizontal body position, then inhaled 3% CO2 for 10 min under HDT. The last 6 min of data were used for analysis in each stage. Arterial pressure waveforms were obtained using finger blood pressure, and blood velocity waveforms in the middle cerebral artery were obtained using transcranial Doppler ultrasonography. Dynamic cerebral autoregulation was evaluated by transfer function analysis between waveforms. Statistical analysis was performed by two‐way repeated‐measures analysis of variance. The index of transfer function gain in the low‐frequency range increased significantly with −15°HDT+CO2 and −30°HDT+CO2, but no changes were seen with −5°HDT+CO2. Phase in the low‐frequency range decreased significantly with all three protocols. These results of significant changes in indexes of both gain and phase during combined exposure to steep HDT (−15° to −30°) and 3% CO2 inhalation suggest weakened dynamic cerebral autoregulation with the combination of moderate cephalad fluid shift and mild hypercapnia.
Thrombosed persistent median artery in a medical sonographer
Authors: Leinfelder Stephen , Nacey Nicholas C 
Published: September 2024

The use of gray-scale and color Doppler ultrasound as a first-line imaging modality for wrist pain can be advantageous, especially in cases of clinically suspected carpal tunnel syndrome. This report presents the case of a medical sonographer who exhibited acute wrist pain secondary to a thrombosed persistent median artery after excessive stress loading on her scanningwrist. Ultrasound is able to easily diagnose this rare condition, which may at times be associated with carpal tunnel symptoms due to mass effect from the expanded aberrant artery.