bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remote C−H Activation of Various N‐Heterocycles Using a Single Template
Authors: Yang Guoqiang , Zhu Dajian , Wang Peng , Tang Ri‐Yuan , Yu Jin‐Quan 
Published: January 2018

AbstractA single and simple ortho‐sulfonyl benzonitrile template was developed to achieve remote C−H olefination of six different classes of N‐heterocycles. We demonstrate that, by varying precatalysts and conditions, the same template can be applied to the remote C−H activation of six structurally distinct heterocyclic scaffolds, and the site‐selectivity can be predicted based on distance and geometry. Furthermore, this new development shows that template‐directed remote C−H activation is possible through macrocyclopalladation processes with smaller ring sizes.
Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study
Authors: Zeng Yang , Dabay Michelle , George Virginia , Seetharaman Shalini , de Arruda Indig Monika , Graminske Sharon , Kimpel Nicole , Schmidt Anna , Boerner Amanda , Paradiso Sarai , Delman Tatyana , Li Yunyao , Litvak Viktoriya , Oreizy Farzad , Chen Angela , Saleminik Maryam , Mosqueda Fred , Lin Anna , Judge Kevin 
Published: January 2018

AbstractThe BD FACSVia™ System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount™ kit with the BD FACSCalibur™ flow cytometer. Leucoreduced platelet (PLT, n = 252) and red blood cell (RBC, n = 278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter‐site reproducibility on the BD FACSVia System at three sites over 20 days. Deming regression and Bland–Altman analysis were performed to determine the WBC absolute counts on the BD FACSVia System vs. the BD FACSCalibur system. Assay accuracy for the range of 0–350 WBCs/µl was adequate. For samples with <25 WBCs/µl, the bias with 95% limits of agreement was 0.136 (–1.897 to 2.169) WBC/µl for PLTs (n = 184) and 0.170 (–2.025 to 2.365) WBC/µl for RBCs (n = 193). For inter‐site reproducibility, the CV% was 6.46% (upper 95% CI 7.16%) for the PLT high control and 9.49% (10.52%) for the PLT low control. The CV% was 7.51% (8.32%) for the RBC high control and 10.76% (11.92%) for the RBC low control. The BD FACSVia System reported equivalent results of WBC absolute counts for leucoreduced PLT and RBC samples compared to the BD FACSCalibur system. The inter‐laboratory reproducibility of the BD FACSVia System met study specifications. © 2018 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC.
Joint service function chain deploying and path selection for bandwidth saving and VNF reuse
Authors: Li Dan , Lan Julong , Wang Peng 
Published: January 2018

SummaryService function chain (SFC) is managed as a set of chained virtual network functions (VNFs) in a particular order. The efficiency of a network closely depends on how these VNFs are deployed in the network. In this paper, we study the joint problem of service function chain deploying and path selection for bandwidth saving and VNF reuse. We first describes the problem as a multiobjective and multirestriction problem, and then a heuristic service function chain deployment algorithm based on longest function assignment sequence is proposed. Via simulations, we show that our design can reduce the number of deployed VNFs and link bandwidth requirement jointly and serve more requests than other algorithms.
Maternal care affects chicks’ development differently according to sex in quail
Authors: Aigueperse Nadège , Pittet Florent , Nicolle Céline , Houdelier Cécilia , Lumineau Sophie 
Published: January 2018

AbstractMaternal behavior is known to influence the behavioral development of young. Recently, it was demonstrated that maternal behavior also differed according to sex of chicks and brood sex composition. So, here, we explored if these factors influenced behavioral development of quail chicks when they were brooded, and what characteristics of chicks and foster females could best explain this development. We studied three sets of chick pairs brooded by foster females: unisex male, unisex female, and mixed broods. We found that both emotivity profile and sociality depended on the sex: females were more reactive and less social than males. Females’ emotivity profile was correlated with brood composition and foster female activity during maternal care. In males, only sociality was correlated with foster females’ scores of aggressive rejection. Our results evidence that male and female chicks respond differentially to maternal behavior. This is discussed in terms of ecological and physiological constraints on development according to sex.
A Collaborative Learning Automata Team Model for Modeling Multi‐Agent Systems
Authors: WANG CEN , KOAKUTSU SEIICHI , OKAMOTO TAKASHI , QIAN FEI 
Published: January 2018

SUMMARYThe learning automaton (LA) team model has been proposed as one method for modeling multi‐agent systems. It is modeled as a noncooperative game of learning automata. In this model, each LA operates independently from each other, and there exists a Nash equilibrium, that is, the existence of an optimal mixed strategy in the mixed strategy space of the game has been proven. However, for modeling multi‐agent systems more generally, the information exchange among agents and the acquisition of cooperative behaviors such as the formation of autonomous community are required. In this paper, in order to complement the LA team model, we propose a new LA team model with some fully or partially collaborative learning behaviors. In this new model, each automaton performs reinforcement learning process in order to identify random environments exchanging information with its adjacent automata. Several computer simulations indicate the availability of the proposed model.
Review about the Development of the Differential Expression QCM System
Authors: SHINOBU WAKAKO , KUKITA HIROYUKI , WAKAMATSU SHUNICHI 
Published: January 2018

SUMMARYUtilization of biosensors has acquired importance in the field of biomedicine, food safety, and environmental monitoring. This has led to the invention of precise analytical tools using biological sensing element as biosensor. This review provides an overview of different types of biosensors being used QCM method from electrochemical for various biomedical with future outlook of biosensor technology.
Multilayer Pb(Zr,Ti)O3 Thin Films for Ultrasonic Transducer
Authors: NAKAMOTO SHOMA , SANO RYO , KANDA KENSUKE , FUJITA TAKAYUKI , MAENAKA KAZUSUKE 
Published: January 2018

SUMMARYIn this study, we have fabricated membranes which are composed of two‐ and four‐layer Pb(Zr,Ti)O3 thin films with internal electrodes by using sputtering depositions. Electrical and driving characteristics as ultrasonic transducers of these membranes are evaluated. By increasing number of layers, the static displacement is reduced. However, for the multilayered membrane, the dynamic displacement became large because of the large mechanical Q factor in resonant vibration. The emitted sound pressure level and characteristics of the ultrasonic transducer are estimated.
MALAT1: A regulator of inflammatory cytokines in diabetic complications
Authors: Gordon Andrew Devon , Biswas Saumik , Feng Biao , Chakrabarti Subrata 
Published: January 2018

SummaryObjectives and DesignIn this study, we examined the role of MALAT1, a highly conserved nuclear long non‐coding RNA molecule, in chronic diabetic complications affecting the heart and kidneys using both in vitro and in vivo models: human endothelial cell culture and a Malat1 knockout mice model.ResultsFindings from our in vitro experiments demonstrated that MALAT1 was predominantly localized to nuclear speckles in endothelial cells and MALAT1 expression was significantly increased following incubation with high glucose in association with increased expression of inflammatory cytokines. As for our in vivo experiments, we used Malat1 knockout mice and wild‐type controls with or without streptozotocin‐induced diabetes over 2 months of follow‐up, where all of our diabetic animals showed hyperglycaemia and polyuria. Examination of cardiac and renal tissues demonstrated altered MALAT1 RNA expression in wild‐type diabetic animals. Such changes were associated with augmented production of downstream inflammatory molecules at the mRNA and protein levels. Diabetes‐induced elevations of inflammatory markers were significantly decreased in Malat1 knockout diabetic animals. In addition to transcript and protein analyses, we examined functional changes in the heart and kidneys. Organ functions were affected in the wild‐type diabetic mice but were rescued in Malat1 knockout mice.ConclusionsTaken together, findings from this study will provide direct evidence and insight into the importance of MALAT1 in the pathogenesis of chronic diabetic complications involving the heart and kidneys.
Single‐dose intravenous iron in Southeast Asian heart failure patients: A pilot randomized placebo‐controlled study (PRACTICE‐ASIA‐HF)
Authors: Yeo Tee Joo , Yeo Poh Shuan Daniel , Hadi Farid Abdul , Cushway Timothy , Lee Kim Yee , Yin Fang Fang , Ching Anne , Li Ruili , Loh Seet Yoong , Lim Shir Lynn , Wong Raymond Ching‐Chiew , Tai Bee Choo , Richards Arthur Mark , Lam Carolyn S.P. 
Published: January 2018

AbstractAimsIron deficiency is highly prevalent in Southeast Asians with heart failure (HF) and associated with worse outcomes. This trial aimed to assess the effect of intravenous iron in Southeast Asians hospitalized with decompensated HF.Methods and resultsFifty patients hospitalized for acute decompensated HF, regardless of ejection fraction, with iron deficiency (defined as serum ferritin <300 ng/mL if transferrin saturation is <20%) were randomized to receive either one dose of intravenous ferric carboxymaltose (FCM) 1000 mg or placebo (0.9% saline) following HF stabilization and before discharge in two Singapore tertiary centres. The primary endpoint was difference in 6‐min walk test (6MWT) distance over 12 weeks, while secondary endpoints were quality of life assessed using validated Kansas City Cardiomyopathy Questionnaire (KCCQ) and Visual Analogue Scale (VAS). Improvement in 6MWT distance at Week 12 was observed in both FCM and placebo groups (from 252 ± 123 to 334 ± 128 m and from 243 ± 67 to 301 ± 83 m, respectively). Unadjusted analysis showed 6MWT distance for FCM exceeded that for placebo, but adjustment for baseline covariates and time attenuated this effect {adjusted mean difference between groups: 0.88 m [95% confidence interval (CI) −30.2 to 32.0, P = 0.956]}. KCCQ overall summary and VAS were similar in both groups [adjusted mean difference: KCCQ −1.48 (95% CI −8.27 to 5.31, P = 0.670) and VAS 0.26 (95% CI −0.33 to 0.86, P = 0.386)]. FCM was well tolerated with no serious treatment‐related adverse events.ConclusionsIntravenous FCM administered pre‐discharge in Southeast Asians hospitalized with decompensated HF is clinically feasible. Changes in 6MWT distance should be measured beyond Week 12 to account for background therapy effects.
Separation/Concentration‐signal‐amplification in‐One Method Based on Electrochemical Conversion of Magnetic Nanoparticles for Electrochemical Biosensing
Authors: Cao Lulu , Zhang Qi , Dai Huang , Fu Yingchun , Li Yanbin 
Published: January 2018

AbstractWe propose a separation/concentration‐signal‐amplification in‐one method based on electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to develop a facile and sensitive electrochemical biosensor for chloramphenicol (CAP) detection. Briefly, aptamer‐modified magnetic nanoparticles (MNPs‐Apt) was designed to capture CAP in sample, then the MNPs‐Apt composite was conjugated to Au electrode through the DNA hybridization between the unoccupied aptamer and a strand of complementary DNA. The ECC method was applied to transfer MNPs labels to electrochemically active Prussian blue (PB). The anodic and cathodic currents of PB were taken for signal readout. Comparing with conventional methods that require electrochemically active labels and related sophisticated labelling procedures, this method explored and integrated the magnetic and electrochemical properties of MNPs into one system, in turn realized magnetic capturing of CAP and signal generation without any additional conventional labels. Taking advantages of the high abundance of iron content in MNPs and the refreshing effect deriving from ECC process, the method significantly promoted the signal amplification. Therefore, the proposed biosensors exhibited linear detection range from 1 to 1000 ng mL−1 and a limit of detection down to 1 ng mL−1, which was better than or comparable with those of most analogues, as well as satisfactory specificity, storage stability and feasibility for real samples. The developed method may lead to new concept for rapid and facile biosensing in food safety, clinic diagnose/therapy and environmental monitoring fields.
Back Cover: In‐vitro analysis of early calcification in aortic valvular interstitial cells using Laser‐Induced Breakdown Spectroscopy (LIBS) (J. Biophotonics 1/2018)
Authors: Davari Seyyed Ali , Masjedi Shirin , Ferdous Zannatul , Mukherjee Dibyendu 
Published: January 2018

Quantitative laser‐induced breakdown spectroscopy (LIBS) is successfully used for in‐vitro analysis of early stage calcification in aortic valvular interstitial cells (VICs). LIBS results indicate 5‐fold improvement in the detection limit of calcium deposition in VICs over cell histology techniques involving staining and colorimetric calcium assays. These results can establish LIBS at the forefront of early detection of calcification in VICs for pathological studies on Calcific Aortic Valve Disease (CAVD). Further details can be found in the article by Seyyed Ali Davari et al. (e201600288).
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Thein vitrorelease of cytokines and growth factors from fibrin membranes produced through horizontal centrifugation
Authors: Lourenço Emanuelle Stellet , Mourão Carlos Fernando de Almeida Barros , Leite Paulo Emílio Corrêa , Granjeiro José Mauro , Calasans‐Maia Mônica Diuana , Alves Gutemberg Gomes 
Published: January 2018

AbstractPlatelet‐rich fibrin membranes are biomaterials widely used for therapeutic purposes, and canonically produced through the processing of peripheral blood with fixed‐angle rotor centrifuges. In this work, we evaluate thein vitrostability and release of cytokines and growth factors when these biomaterials are produced with a horizontal swing‐out clinical centrifuge. Membranes produced from the blood of 14 donors were morphologically evaluated by scanning electron microscopy and fluorescence microscopy, and their stability was assessed by photographic recording after incubation in culture medium for up to 28 days. The release of 27 cytokines and growth factors was monitored for three weeks through a multiparametric immunoassay. The fibrin membranes presented complex three‐dimensional structure with a high density of nucleated cells. A large release of growth factors [platelet derived growth factor, fibroblastic growth factor (bFGF), and vascular endothelial growth factor] was detected in the first 24 h, followed by time‐dependent decay, maintaining significant concentrations after three weeks. Both anti‐inflammatory and pro‐inflammatory cytokines presented different release peaks, maintaining high rates of elution for up to 21 days. Chemokines of relevance in tissue repair [RANTES, granulocyte colony‐stimulating factor (G‐CSF)] were also produced in large quantities throughout the experimental period. The present results demonstrate that blood‐derived fibrin membranes with high structural stability and cell content can be generated by horizontal centrifugation, being able of a prolonged production/release of growth factors and pro‐ and anti‐inflammatory cytokines. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1373–1380, 2018.
Heat shock protein 70 modulates neural progenitor cells dynamics in human neuroblastoma SH‐SY5Y cells exposed to high glucose content
Authors: Salimi Leila , Rahbarghazi Reza , Jafarian Vahab , Biray Avci Çıgır , Goker Bagca Bakiye , Pinar Ozates Neslihan , Khaksar Majid , Nourazarian Alireza 
Published: January 2018

AbstractIn the current experiment, detrimental effects of high glucose condition were investigated on human neuroblastoma cells. Human neuroblastoma cell line SH‐SY5Y were exposed to 5, 40, and 70 mM glucose over a period of 72 h. Survival rate and the proliferation of cells were analyzed by MTT and BrdU incorporation assays. Apoptosis was studied by the assays of flow cytometry and PCR array. In order to investigate the trans‐differentiation capacity of the cell into mature neurons, we used immunofluorescence imaging to follow NeuN protein level. The transcription level of HSP70 was shown by real‐time PCR analysis. MMP‐2 and −9 activities were shown by gelatin Zymography. According to data from MTT and BrdU incorporation assay, 70 mM glucose reduced cell viability and proliferation rate as compared to control (5 mM glucose) and cells treated with 40 mM glucose (P < 0.05). Cell exposure to 70 mM glucose had potential to induced apoptosis after 72 h (P < 0.05). Our results also demonstrated the sensitivity of SH‐SY5Y cells to detrimental effects of high glucose condition during trans‐differentiation into mature neuron‐like cells. Real‐time PCR analysis confirmed the expression of HSP70 in cells under high content glucose levels, demonstrating the possible cell compensatory response to an insulting condition (pcontrol vs 70 mM group <0.05). Both MMP‐2 and ‐9 activities were reduced in cells being exposed to 70 mM glucose. High glucose condition could abrogate the dynamics of neural progenitor cells. The intracellular level of HSP70 was proportional to cell damage in high glucose condition.
Probing the microscopic environment of 23Na ions in brain tissue by MRI: On the accuracy of different sampling schemes for the determination of rapid, biexponential decay at low signal‐to‐noise ratio
Authors: Lommen Jonathan M. , Flassbeck Sebastian , Behl Nicolas G.R. , Niesporek Sebastian , Bachert Peter , Ladd Mark E. , Nagel Armin M. 
Published: January 2018

PurposeTo investigate and to reduce influences on the determination of the short and long apparent transverse relaxation times (
,
) of 23Na in vivo with respect to signal sampling.MethodsThe accuracy of determination was analyzed in simulations for five different sampling schemes. The influence of noise in the parameter fit was investigated for three different models. A dedicated sampling scheme was developed for brain parenchyma by numerically optimizing the parameter estimation. This scheme was compared in vivo to linear sampling at 7T.ResultsFor the considered sampling schemes,
/
exhibit an average bias of 3% / 4% with a variation of 25% / 15% based on simulations with previously published
values. The accuracy could be improved with the optimized sampling scheme by strongly averaging the earliest sample. A fitting model with constant noise floor can increase accuracy while additional fitting of a noise term is only beneficial in case of sampling until late echo time > 80 ms.
values in white matter were determined to be
= 5.1 ± 0.8 / 4.2 ± 0.4 ms and
= 35.7 ± 2.4 / 34.4 ± 1.5 ms using linear/optimized sampling.ConclusionVoxel‐wise determination of 23Na is feasible in vivo. However, sampling and fitting methods have to be chosen carefully to retrieve accurate results. Magn Reson Med 80:571–584, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
In vivo estimation of transverse relaxation time constant (T2) of 17 human brain metabolites at 3T
Authors: Wyss Patrik O. , Bianchini Claudio , Scheidegger Milan , Giapitzakis Ioannis A. , Hock Andreas , Fuchs Alexander , Henning Anke 
Published: January 2018

PurposeThe transverse relaxation times T2 of 17 metabolites in vivo at 3T is reported and region specific differences are addressed.MethodsAn echo‐time series protocol was applied to one, two, or three volumes of interest with different fraction of white and gray matter including a total number of 106 healthy volunteers and acquiring a total number of 128 spectra. The data were fitted with the 2D fitting tool ProFit2, which included individual line shape modeling for all metabolites and allowed the T2 calculation of 28 moieties of 17 metabolites.ResultsThe T2 of 10 metabolites and their moieties have been reported for the first time. Region specific T2 differences in white and gray matter enriched tissue occur in 16 of 17 metabolites examined including single resonance lines and coupled spin systems.ConclusionThe relaxation time T2 is regions specific and has to be considered when applying tissue composition correction for internal water referencing. Magn Reson Med 80:452–461, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
Self‐gated golden‐angle spiral 4D flow MRI
Authors: Bastkowski Rene , Weiss Kilian , Maintz David , Giese Daniel 
Published: January 2018

PurposeThe acquisition of 4D flow magnetic resonance imaging (MRI) in cardiovascular applications has recently made large progress toward clinical feasibility. The need for simultaneous compensation of cardiac and breathing motion still poses a challenge for widespread clinical use. Especially, breathing motion, addressed by gating approaches, can lead to unpredictable and long scan times. The current work proposes a time‐efficient self‐gated 4D flow sequence that exploits up to 100% of the acquired data and operates at a predictable scan time.MethodsA self‐gated golden‐angle spiral 4D flow sequence was implemented and tested in 10 volunteers. Data were retrospectively binned into respiratory and cardiac states and reconstructed using a conjugate‐gradient sensitivity encoding reconstruction. Net flow curves, stroke volumes, and peak flow in the aorta were evaluated and compared to a conventional Cartesian 4D flow sequence. Additionally, flow quantities reconstructed from 50% to 100% of the self‐gated 4D flow data were compared.ResultsSelf‐gating signals for respiratory and cardiac motion were extracted for all volunteers. Flow quantities were in agreement with the standard Cartesian scan. Mean differences in stroke volumes and peak flow of 7.6 ± 11.5 and 4.0 ± 79.9 mL/s were obtained, respectively. By retrospectively increasing breathing navigator efficiency while decreasing acquisition times (15:06–07:33 minutes), 50% of the acquired data were sufficient to measure stroke volumes with errors under 9.6 mL.ConclusionThe feasibility to acquire respiratory and cardiac self‐gated 4D flow data at a predictable scan time was demonstrated. Magn Reson Med 80:904–913, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
A block GMRES method with deflated restarting for solving linear systems with multiple shifts and multiple right‐hand sides
Authors: Sun Dong‐Lin , Huang Ting‐Zhu , Jing Yan‐Fei , Carpentieri Bruno 
Published: January 2018

SummaryThe restarted block generalized minimum residual method (BGMRES) with deflated restarting (BGMRES‐DR) was proposed by Morgan to dump the negative effect of small eigenvalues from the convergence of the BGMRES method. More recently, Wu et al. introduced the shifted BGMRES method (BGMRES‐Sh) for solving the sequence of linear systems with multiple shifts and multiple right‐hand sides. In this paper, a new shifted block Krylov subspace algorithm that combines the characteristics of both the BGMRES‐DR and the BGMRES‐Sh methods is proposed. Moreover, our method is enhanced with a seed selection strategy to handle the case of almost linear dependence of the right‐hand sides. Numerical experiments illustrate the potential of the proposed method to solve efficiently the sequence of linear systems with multiple shifts and multiple right‐hand sides, with and without preconditioner, also against other state‐of‐the‐art solvers.