bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brody's abscess of the humerus is the cause of sepsis in a patient with coronavirus infection (COVID-19)
Authors: Kislov M. A. , Maksimov A. V. , Supilnikov A. A. 
Published: October 2024

Purpose: To conduct a clinical and morphological analysis of the case of death as a result of sepsis, the cause of which was Brody's abscess.Material and methods. An analysis of the fatal outcome of a 54–year-old patient who occurred in a hospital of a medical organization where he received medical care for a disease – a new coronavirus infection. The complex of clinical, laboratory and instrumental diagnostic methods could not explain the progressive deterioration of the severity of the patient's condition and the onset of death.Results. During the autopsy, an abscess of the proximal epiphysis of the left humerus was found with the spread of the inflammatory process to the soft tissues of the breast, the parietal pleura with the development of purulent fibrinous pleurisy, left-sided pneumonia, purulent fibrinous pericarditis, hematogenous purulent meningitis.Conclusion. The authors of the article have established a causal cause of death from Brody's abscess, which makes it possible to expand the diagnostic search in the case of sepsis with an unclear primary focus.
Navigation on Temporal Networks
Authors: Robledo Omar F. , Holme Petter , Wang Huijuan 
Published: October 2024

Abstract

Temporal networks, whose network topology changes over time, are used to represent, e.g., opportunistic mobile networks, vehicle networks, and social contact networks, where two mobile devices (autos or individuals) are connected only when they are close to (interact with) each other. Such networks facilitate the transfer of information. In this paper, we address the problem of navigation on temporal networks: how to route a traffic demand from a source s to a destination d at time ts, based on the network observed before ts? Whenever the node hosting the information has a contact or interacts with another node, the routing method has to decide whether the information should be forwarded to the contacted node or not. Once the information is forwarded, the contacted node becomes the only node hosting the information. Firstly, we introduce a framework of designing navigation algorithms, in which a distance metric is defined and computed between any node to the target d based on the network observed before ts. Whenever a hosting node has a contact, it forwards the information to the contacted node if the contacted node is closer to the target than the hosting node according to the distance metric. Secondly, we propose systematically distance metrics of a node pair in the temporal network observed, that capture different network properties of a node pair. Thirdly, these metrics or routing strategies are evaluated in empirical contact networks, from the perspective of the time duration of the routing and the probability that the destination can be reached. Their performance is further explained via the correlation between distance metrics and the stability of each metric in ranking nodes' distance to a target node. These contributions are deemed as the first step towards navigation on realistic mobile, vehicle or social networks.
Recycling of polystyrene waste (thermocol) using pyrolysis with nano catalyst process into valuable product like fuel.
Authors: Giri Pradnya , Waghmode Shobha , Chapkanade Priyanka , Gadale Sharda 
Published: October 2024

Abstract

Marketed under the trade name Thermocol, expanded polystyrene (EPS) consists of 90–95% polystyrene and 5–10% gaseous blowing agent, such as carbon dioxide or pentane. This polymer is highly consumed because of its many applications across several industries, especially packaging. Owing to its large volume and lack of biodegradability, it makes up a significant portion of the waste stream from municipalities. Long-chain polymer molecules are broken down into small-chain molecules by the pyrolysis process, which requires heat energy. Smaller molecules can be produced by adjusting the reaction's pressure and heat flow. Converting waste plastic into fuel is the most environmentally beneficial way to safeguard the environment since it recovers the organic component of polymeric waste in the form of valuable petroleum products. A study was conducted on dolomite, which is commercially available, and two different nanocatalysts, CaO:MgO and ZnO:CaO:MgO, synthesized in the laboratory. The use of these synthesized nanocatalysts resulted in an increased yield of the oil product than dolomite is used as a catalyst in pyrolysis.
The REV1-Targeting Inhibitor JH-RE-06 Triggers Programmed Cell Death by Regulating NCOA4-Mediated Ferritinophagy and the KEAP1-NRF2-ARE Pathway in CRC Cells
Authors: Cheng Jianhua , Yang Xiaoxia , Zhao Wen , Xu Jie , Hao Yanjie , Wang Feimiao , Xu Fang 
Published: October 2024

Abstract

Oncogenes accelerate DNA replication, triggering excessive replication origin activation. This leads to DNA replication stress and genomic instability in cancer cells, making replication stress a potential therapeutic target. Translesion synthesis (TLS) serves as a compensation mechanism for replication stress, enabling cancer cells to acquire a growth advantage. Cancer cells exploit translesion synthesis (TLS) to circumvent DNA adducts produced by platinum-based chemotherapeutics, leading to the emergence of resistance. Consequently, targeting TLS offers a dual advantage for oncological therapy. The application of the translesion synthesis polymerase REV1 inhibitor JH-RE-06 in the treatment of colorectal cancer (CRC) remains unexplored. The molecular mechanisms by which JH-RE-06 induces programmed cell death have also not been reported. Our findings revealed that JH-RE-06 could trigger programmed cell death in CRC cells.Specifically, JH-RE-06 enhances the level of cleaved caspase-3 and cleaved PARP1 in cancer cells, stimulates NCOA4-mediated ferritinophagy, which results in ferroptosis. Cells activate the KEAP1-NRF2 pathway in response to the oxidative stress caused by JH-RE-06. This programmed cell death (PCD) can be reversed by cysteine-synthesizing pharmaceuticals. While JH-RE-06 does not increase the sensitivity of CRC cells to oxaliplatin, it effectively suppresses clonal proliferation of oxaliplatin-resistant cell lines in vitro and inhibits oxaliplatin-resistant xenograft tumors growth in vivo. The data indicate that JH-RE-06 may serve as a viable second-line chemotherapeutic treatment for CRC in cases of chemoresistance.
Research on the Safety Assessment Method of Coal Mine Gas Situations Using G1-CRITIC Empowered Cloud Model
Authors: Zhang Wenke 
Published: October 2024

Abstract

Scientifically evaluating the safety status of coal mine gas is crucial for effective gas risk management. This paper introduces the WSR theory to analyze the influencing factors of coal mine gas situations across the Wuli, Shili, and Renli dimensions. The G1 method and an improved CRITIC method are employed to calculate the subjective and objective weights of gas situation indicators, respectively. A distance function discrimination method is introduced to achieve comprehensive indicator weighting. Combining these methods with cloud model theory, a cloud model for coal mine gas situation safety assessment is constructed and validated through a case study. The results indicate that the assessment method based on the G1-CRITIC empowered cloud model effectively evaluates the gas safety status of the No. 3 coal seam in Wangzhuang Mine, with a safety level classified as Level II, consistent with the actual situation. This confirms the model’s applicability for assessing coal mine gas safety situations.
Grey Frequency-Based Methodology for Assessing HeLa Cell Damage
Authors: Li Anqi , Zhao Linying , Liu Changyu , Xu Xiaolong , Jia Jianbo 
Published: October 2024

Abstract

Cell biology techniques offer a solid foundation for evaluating and forecasting the danger of pollutants in the investigations of environmental toxicology. Studies on ecological toxicity, medication development, and illness diagnosis depend on evaluating cellular damage. The morphology of stimulated cells can alter the light scattering and reflection, and the brightness of microscopic images of the cells. This study demonstrated that stimulation-damaged and normal cells had distinct grey value distributions which led to the proposal of a novel theory to measure cellular damage by image brightness. Additionally, an evaluation technique based on grey frequency analysis can be created to determine the extent of cellular damage. This approach provides an effective and helpful tool for cellular damage visualization and quantitative evaluation in environmental toxicity assessment.
Inhibition and Kinetic Studies on Purified Peroxidase from Rhizome of Turmeric (Curcuma Longa L)
Authors: Adedugbe Omowumi Funke , Ilesanmi Olutosin Samuel , Olagunju Victory Ayo , Kolawole Ayodele O. 
Published: October 2024

Abstract

The kinetics, inhibition studies and the interactions of inhibitors and substrates with purified peroxidase from rhizomes of turmeric (Curcuma longa) (ClP) through molecular docking was described. This was with the view to providing information on the catalytic mechanism of the enzyme with substrate and inhibitors for various applications. The crude enzyme was purified in single step purification using aqueous two-phase partitioning system (ATPS). Real kinetic studies on the purified enzyme showed linear patterns with intersection on the x-axis in the third quadrant suggesting sequential ordered bi bi mechanism of substrate addition to the peroxidase. The real kinetic constants − Kmcatechol and KmH2O2 estimated from the secondary replots for the purified peroxidase from turmeric were 168 ± 2.0 mM and 87.4 ± 1.2 mM respectively. The Vmax obtained for the purified enzyme was 68,965 ± 50 units/mg protein. These led to first-order rate constant, kcat/Km of 0.49 × 106 M− 1 s− 1. All the inhibitors had inhibitory effect on the activity of ClP at varying concentrations. The inhibition constant (Ki) values for the inhibitors at increasing order are 0.4 mM for cysteine, 4.9 mM for ascorbic acid, 5 mM for citric acid and 9 mM for EDTA. Cysteine was the most potent inhibitor. From the docking simulation, the calculated docking score of the binding energy for ascorbic acid, citric acid, cysteine and EDTA were − 8.988, -4.147, -3.361 and − 2.206 kcal/mol respectively. The lower binding energy value of the inhibitor represents the higher affinity to the receptor protein. The binding interaction of the purified enzyme showed that ascorbic acid, citric acid and EDTA have 2 hydrogen bonds formed respectively while cysteine had 4 hydrogen bonds. The combination of kinetic and inhibition properties makes the enzyme a successful candidate to be employed for various applications in industrial and biotechnological processes.
Global trends and focuses of Integrins in Colorectal Cancer: a bibliometric analysis and visualization from 2007 to 2023
Authors: Yin Wen , Xiao Lijun , Zhang Chao , Zhang Xu , Wen Chengli , Li Hao , Yu Yuan , Yu Zehui , Lü Muhan 
Published: October 2024

Abstract

Colorectal cancer is one of the most common tumors of the digestive system; however, current treatment methods still have certain limitations. In recent years, researchers have found that integrins play a crucial role in colorectal cancer, making them a hot topic in research. To gain a better understanding of the current status and trends of integrins in colorectal cancer research, this study conducted a bibliometric analysis for the first time to assess the development of integrin-related research in colorectal cancer. The study selected the core dataset from the Web of Science database and included relevant research on integrins and colorectal cancer published between 2007 and 2023. The CiteSpace, VoSviewer, and Bibliometrix R packages were utilized for analyzing the aspects of country/region, authors, institutions, journals, references, and keywords. The analysis revealed steady growth in research on integrins and colorectal cancer over the past 16 years, with a total of 1,339 articles published by 61 countries. Among them, China has the highest number of publications, followed by the United States. The most frequently cited journal is Cancer Research, followed by the Journal of Biological Chemistry and Oncogene. In conclusion, bibliometric analysis provided a comprehensive visualization of the current status and trends in research on integrins in colorectal cancer. These analytical results not only offer valuable insights for researchers but also provide useful information for decision-makers in the scientific and medical communities. This, in turn, can facilitate the improvement and development of treatment methods for colorectal cancer.
Public views on genetics and genetic testing of sudden unexplained death: A survey of the public in China
Authors: Qi Ming , Hong Xutao , Chen Xiaoling , He Yanbin , Wang Jinyuan , Huang Jian , Xu Xiaoyan , Zhang Jungeng , Dong Shuangyong , Wang JianGang 
Published: October 2024

Abstract

This study investigated public attitudes towards genetic testing for sudden cardiac death (SCD) in China, particularly in the context of increasing cardiovascular disease incidence and the potential benefits of genetic testing for prevention and treatment. We conducted a telephone follow-ups targeted 199 out-of-hospital cardiac arrest (OHCA) cases in Hangzhou, and a public survey included 536 respondents from various demographic groups, including emergency volunteers, housewives, IT practitioners, Cardiovascular patients' relatives, and drivers. The telephone follow-up revealed a low participation rate in genetic testing among survivors and victims' immediate relatives. The public survey indicated a general lack of awareness and knowledge about genetics and genetic testing for SCD. Significant differences in attitudes were observed based on demographic factors such as age, health insurance, and occupations. Many respondents expressed concerns about the implications of genetic testing and a general distrust in medical recommendations. The study highlights the urgent need for enhanced public education on the importance of genetic testing for SCD and the development of more effective communication strategies to address public concerns. Increasing awareness and understanding of the benefits of genetic testing could improve acceptance and participation, ultimately aiding in the prevention and management of SCD in China.
Total Darkness Activated Intestinal Clock System and Improved Intestinal Barrier Function in Growing Rabbits
Authors: Li Yao , Chen Jiali , Li Fuchang , Liu Lei 
Published: October 2024

Abstract

The aim of study was to investigate the effects of dark environment on production performance, intestinal barrier function and clock-related gene expression in rabbits. Forty weaned rabbits with similar body weight (35-day-old) were randomly divided into 2 treatments (20 replicates per treatment, 1 rabbit per replicate: normal light group (12 L and 12 D) or total dark group (24 D). The experimental period lasted for 10 days, with an adaptation period of 3 days and a subsequent formal experimental period of 7 days. The results showed that feed-to-weight ratio of rabbits in total dark group was significantly decreased compared with normal light group (P<0.05). Dark treatment significantly increased gene expression of claudin-1, mucin1 in duodenum, occludin-1, claudin-1, zona occludens 1 (ZO1), junctional adhesion molecule 2 (JAM2) and interleukin 10 (IL10) in jejunum, claudin-1, mucin1, ZO1 and IL10 in ileum and clock, melatonin 1A, melatonin 1B, and period1 in cecum compared with normal light group (P<0.05). Total dark treatment increased alpha diversity via increasing chao1 index, observed species index and faith_pd index of cecal flora. Total dark treatment significantly reduced percentage of Deferobacterium at phylum level in cecum, but significantly increased percentage of Rumenococci at genus level (P<0.05). There is an increasing tendency of acetic acid and propionic acid content of soft feces in total dark group (0.05<P<0.1). In conclusion, total dark treatment improves feed conversion efficiency in rabbits and activates cecum clock system, which increased diversity of bacterial flora and production of short-chain fatty acids, then increases intestinal barrier function.
Mitochondrial Dysfunction and Programmed Cell Death in Allergic Rhinitis: Potential Biomarkers and Therapeutic Targets
Authors: Xie Yong , Liang Meiying , Fang Meiqing , Ling Tao , Kong Hui 
Published: October 2024

Abstract

Background
Allergic rhinitis (AR) is a prevalent chronic inflammatory disease, and its pathological mechanisms have not been fully elucidated. This study aims to identify potential biomarkers of AR and explore its role in disease development through integrated bioinformatics analysis.
Methods
We downloaded GSE75011 and GSE46171 datasets from public database to screen out differentially expressed genes (DEGs) between AR patients and control samples. Using MitoCarta 3.0 and literature appendices, we identified mitochondria-associated genes (MitoRGs) and programmed cell death-related genes (PCDRGs). Using Spearman correlation analysis, we screened out DE MitoRGs-PCDRGs with significant correlations. Further, we constructed a protein-protein interaction (PPI) network using the search tool for the retrieval of interacting genes/proteins (STRING) database and visualized it by Cytoscape software. Using machine learning algorithms, we identified biomarkers of AR from candidate genes. In addition, we analyzed the biological functions and signaling pathways of these biomarkers by Gene Set Enrichment Analysis (GSEA), and assessed the infiltration of immune cells by immunoinfiltration analysis.
Results
We identified a total of 50 AR-associated DE MitoRGs-PCDRGs that were strongly associated with apoptosis. Through machine learning algorithms, we identified HPDL, PLEKHF1, PFKFB3, and TNFAIP3 as potential biomarkers for AR. The distribution of these biomarkers on chromosomes and the strong correlation between them suggested that they might play a synergistic role in the pathogenesis of AR. GSEA analysis reveals the potential role of these biomarkers in immune response and cell signaling. Immunoinfiltration analysis revealed significant differences in immune cells between AR and normal control (NC) samples, which were closely related to the expression levels of biomarkers.
Conclusion
This study reveals potential biomarkers of AR through comprehensive analysis and explores their possible mechanisms in disease development. These findings provide new perspectives for the diagnosis and treatment of AR and lay the foundation for future research and clinical applications.
Body shape, fat distribution and sarcopenia and risk of liver cancer in European population: a Mendelian randomization study
Authors: Liu Yong-Cai , He Chiyu , Zhang Peiru , Hu Zhihang , Lin Zuyuan , Chen Jinyan , Shen Wei , Chen Hao , Li Huigang , Yang Xinyu , Zhuo Jianyong , Wei Xuyong , Zhuang Li , Xu Xiao , Zheng Shusen , Lu Di 
Published: October 2024

Abstract

Background
Body shape, fat and muscle are likely associated with risk of liver cancer. Evidence for the impact of these factor is limited and controversial. Because conventional observational studies cannot sidestep the effect of confounding and reverse causality, it remains unclear whether there is a causal relationship of body shape, composition and its distribution with risk of liver cancer.
Methods
In this study, a two-sample Mendelian randomization (MR) approach was applied to evaluate the potential causal association of 17 risk factors of body shape, fat distribution and sarcopenia with risk of liver cancer in European population. Summary genetic association estimates for 17 exposures and liver cancer were obtained from MRCIEU GWAS database.
Results
MR analysis indicated that genetically predicted body mass index (BMI) and waist-to-hip ratio (WHR) were associated with liver cancer risk [OR: 1.0005, P = 0.037; OR: 1.0014, P = 0.004, respectively]. Whole body fat mass, trunk fat mass, arm fat mass and leg fat mass were associated with liver cancer risk, while the corresponding fat-free mass were not associated with liver cancer risk. Genetically determined sarcopenia was not associated with liver cancer risk, as either. There did not suggest evidence of potential heterogeneity or directional pleiotropy.
Conclusion
Our study found genetically predicted BMI, WHR and fat mass were associated with liver cancer risk, and the positive association between fat mass and liver cancer risk did not change with changes in fat distribution. However, fat-free mass and sarcopenia associated factors were not associated with liver cancer risk.
Enhancing Situational Awareness with LoRa Mesh Networks: Communication in Internet-Deprived Areas
Authors: Hakani Raj , Mishra Aditya , Shah Naman , Rawat Abhishek 
Published: October 2024

Abstract

This paper focuses on the implementation of Meshtastic firmware, showcasing its effectiveness in establishing a decentralized mesh network for communication and location tracking in areas where internet connectivity is unreliable. A thorough analysis of the system's features, including real-time device tracking and secure messaging, highlights its significant potential for military and surveillance applications in remote or off-grid settings. The decentralized mesh framework enhances resilience in dynamic environments, providing a versatile communication solution where conventional infrastructure is lacking or compromised. The experiments successfully demonstrate the real-world application of LoRa communication technology in urban settings, achieving dependable communication between sender and receiver boards over distances of up to 2 kilometers, even in the presence of potential urban interference. Additionally, this research highlights security protocols and ethical considerations relevant to the deployment of Meshtastic firmware in military and surveillance scenarios, offering insightful guidance for future studies and practical implementations.