bluuebunny/crossref_metadata_2025_split huggingface dataset:
Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase
Authors: Ma Junyan , Zhang Weiting , Rahimialiabadi Simin , Ganesh Nikkitha Umesh , Sun Zhengwang , Parvez Saba , Peterson Randall T. , Yeh Jing-Ruey Joanna 
Published: September 2024

ABSTRACT
Here, we introduce ‘TICIT’, targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase – phiC31 integrase – to insert large DNA fragments into CRISPR-Cas9 target loci. This technique, which relies on first knocking in a 39-basepair phiC31 landing site via CRISPR-Cas9, enables researchers to repeatedly perform site-specific transgenesis at the exact genomic location with high precision and efficiency. We applied this approach to devise a method for the instantaneous determination of a zebrafish's genotype simply by examining its color. When a zebrafish mutant line must be propagated as heterozygotes due to homozygous lethality, employing this method allows facile identification of a population of homozygous mutant embryos even before the mutant phenotypes manifest. Thus, it should facilitate various downstream applications, such as large-scale chemical screens. We demonstrated that TICIT could also create reporter fish driven by an endogenous promoter. Further, we identified a landing site in the tyrosinase gene that could support transgene expression in a broad spectrum of tissue and cell types. In sum, TICIT enables site-specific DNA integration without requiring complex donor DNA construction. It can yield consistent transgene expression, facilitate diverse applications in zebrafish, and may be applicable to cells in culture and other model organisms.
The non-canonical bivalent gene Wfdc15a controls spermatogenic protease and immune homeostasis
Authors: Tomizawa Shin-ichi , Fellows Rachel , Ono Michio , Kuroha Kazushige , Dočkal Ivana , Kobayashi Yuki , Minamizawa Keisuke , Natsume Koji , Nakajima Kuniko , Hoshi Ikue , Matsuda Shion , Seki Masahide , Suzuki Yutaka , Aoto Kazushi , Saitsu Hirotomo , Ohbo Kazuyuki 
Published: September 2024

ABSTRACT
Male infertility can be caused by chromosomal abnormalities, mutations and epigenetic defects. Epigenetic modifiers pre-program hundreds of spermatogenic genes in spermatogonial stem cells (SSCs) for expression later in spermatids, but it remains mostly unclear whether and how those genes are involved in fertility. Here, we report that Wfdc15a, a WFDC family protease inhibitor pre-programmed by KMT2B, is essential for spermatogenesis. We found that Wfdc15a is a non-canonical bivalent gene carrying both H3K4me3 and facultative H3K9me3 in SSCs, but is later activated along with the loss of H3K9me3 and acquisition of H3K27ac during meiosis. We show that WFDC15A deficiency causes defective spermiogenesis at the beginning of spermatid elongation. Notably, depletion of WFDC15A causes substantial disturbance of the testicular protease-antiprotease network and leads to an orchitis-like inflammatory response associated with TNFα expression in round spermatids. Together, our results reveal a unique epigenetic program regulating innate immunity crucial for fertility.
ExoJ – a Fiji/ImageJ2 plugin for automated spatiotemporal detection and analysis of exocytosis
Authors: Liu Junjun , Verweij Frederik Johannes , van Niel Guillaume , Galli Thierry , Danglot Lydia , Bun Philippe 
Published: September 2024

ABSTRACT
Exocytosis is a dynamic physiological process that enables the release of biomolecules to the surrounding environment via the fusion of membrane compartments to the plasma membrane. Understanding its mechanisms is crucial, as defects can compromise essential biological functions. The development of pH-sensitive optical reporters alongside fluorescence microscopy enables the assessment of individual vesicle exocytosis events at the cellular level. Manual annotation represents, however, a time-consuming task that is prone to selection biases and human operational errors. Here, we introduce ExoJ, an automated plugin based on Fiji/ImageJ2 software. ExoJ identifies user-defined genuine populations of exocytosis events, recording quantitative features including intensity, apparent size and duration. We designed ExoJ to be fully user-configurable, making it suitable for studying distinct forms of vesicle exocytosis regardless of the imaging quality. Our plugin demonstrates its capabilities by showcasing distinct exocytic dynamics among tetraspanins and vesicular SNARE protein reporters. Assessment of performance on synthetic data shows that ExoJ is a robust tool that is capable of correctly identifying exocytosis events independently of signal-to-noise ratio conditions. We propose ExoJ as a standard solution for future comparative and quantitative studies of exocytosis.
Gut Analysis Toolbox – automating quantitative analysis of enteric neurons
Authors: Sorensen Luke , Humenick Adam , Poon Sabrina S. B. , Han Myat Noe , Mahdavian Narges S. , Rowe Matthew C. , Hamnett Ryan , Gómez-de-Mariscal Estibaliz , Neckel Peter H. , Saito Ayame , Mutunduwe Keith , Glennan Christie , Haase Robert , McQuade Rachel M. , Foong Jaime P. P. , Brookes Simon J. H. , Kaltschmidt Julia A. , Muñoz-Barrutia Arrate , King Sebastian K. , Veldhuis Nicholas A. , Carbone Simona E. , Poole Daniel P. , Rajasekhar Pradeep 
Published: September 2024

ABSTRACT
The enteric nervous system (ENS) consists of an extensive network of neurons and glial cells embedded within the wall of the gastrointestinal (GI) tract. Alterations in neuronal distribution and function are strongly associated with GI dysfunction. Current methods for assessing neuronal distribution suffer from undersampling, partly due to challenges associated with imaging and analyzing large tissue areas, and operator bias due to manual analysis. We present the Gut Analysis Toolbox (GAT), an image analysis tool designed for characterization of enteric neurons and their neurochemical coding using two-dimensional images of GI wholemount preparations. GAT is developed in Fiji, has a user-friendly interface, and offers rapid and accurate segmentation via custom deep learning (DL)-based cell segmentation models developed using StarDist, as well as a ganglia segmentation model in deepImageJ. We apply proximal neighbor-based spatial analysis to reveal differences in cellular distribution across gut regions using a public dataset. In summary, GAT provides an easy-to-use toolbox to streamline routine image analysis tasks in ENS research. GAT enhances throughput, allowing rapid unbiased analysis of larger tissue areas, multiple neuronal markers and numerous samples.
Σχολική Δέσμευση και ικανοποίηση από τη ζωή σε μαθητές Δημοτικού σχολείου
Authors: Τσουβέλας Γιώργος , Παπουλίδη Ασημένια 
Published: September 2024

Προηγούμενες μελέτες εξέταζαν το φαινόμενο της σχολικής δέσμευσης (συμπεριφορική - γνωστική - συναισθηματική), κυρίως, μέσα από μοντέλα ελλειμμάτων, που εστίαζαν σε έννοιες όπως η σχολική διαρροή, οι απουσίες από το σχολικό περιβάλλον αλλά και η σχολική επίδοση. Στο πλαίσιο της παρούσας μελέτης η σχολική δέσμευση προσεγγίζεται μέσα από την οπτική της θετικής ψυχολογίας και της θετικής εκπαίδευσης με στόχο να αναδειχθεί μια νέα αναπτυξιακή προοπτική ενδυνάμωσης και ενίσχυσης των ικανοτήτων των παιδιών και να επεκταθούν οι εκπαιδευτικοί στόχοι προκειμένου να περιλαμβάνουν δράσεις και ενέργειες που προωθούν τη ψυχική ευημερία των μαθητών. Στη μελέτη συμμετείχαν 115 μαθητές της έκτης δημοτικού (44.3% αγόρια) που φοιτούσαν σε ιδιωτικά σχολεία της Αθήνας. Χορηγήθηκαν οι κλίμακες Πολυδιάστατη Κλίμακα ικανοποίησης από τη ζωή για μαθητές, κλίμακα Σχολικής Δέσμευσης και ένα αυτοσχέδιο ερωτηματολόγιο δημογραφικών στοιχείων στο οποίο ζητούνταν από τους μαθητές να αναφέρουν το φύλο, την ημερομηνία γέννησης, το αν έχουν αδέρφια, τον μέσο όρο επίδοσης της προηγούμενης χρονιάς στα μαθήματα των Μαθηματικών και της Γλώσσας και το επίπεδο εκπαίδευσης των γονέων. Η επιβεβαιωτική ανάλυση παραγόντων έδειξε ότι η κλίμακα της σχολικής δέσμευσης εμφάνισε ικανοποιητικούς δείκτες καλής προσαρμογής. Οι παράγοντες της σχολικής δέσμευσης συσχετίστηκαν θετικά με όλους τους παράγοντες της ικανοποίησης από τη ζωή. Οι υψηλότερες συσχετίσεις εντοπίστηκαν ανάμεσα στην ικανοποίηση από το σχολείο και τη συναισθηματική και συμπεριφορική δέσμευση. Διενεργήθηκε ιεραρχική πολλαπλή παλινδρόμηση με τη μέθοδο stepwise προκειμένου να ελεγχθεί η στατιστική πρόβλεψη της ικανοποίησης από το σχολείο από τους δείκτες της σχολικής δέσμευσης και δημογραφικούς παράγοντες. Η ικανοποίηση από το σχολικό περιβάλλον προβλέφθηκε από τους παράγοντες της σχολικής δέσμευσης α) συναισθηματική και β) γνωστική δέσμευση και από το φύλο. Δημογραφικοί και παράγοντες που σχετίζονταν με την επίδοση κατά την προηγούμενη χρονιά δεν συνέβαλαν στην πρόβλεψη της ικανοποίησης από το σχολείο. Όπως προέκυψε από μοντέλο δομικών εξισώσεων η λανθάνουσα μεταβλητή της σχολικής δέσμευσης εμφάνισε πλήρη διαμεσολάβηση ανάμεσα στη σχέση της ικανοποίησης από την οικογένεια και την ικανοποίηση από το σχολείο. Τόσο οι εκπαιδευτικοί όσο και οι πολιτικές εκπαίδευσης θα είναι χρήσιμο να συμπεριλάβουν δράσεις στο αναλυτικό πρόγραμμα και σε διαθεματικές δραστηριότητες για την προώθηση κοινωνικών και συναισθηματικών δεξιοτήτων και αλληλεπιδράσεων που θα έχουν ως αποτέλεσμα τόσο την αύξηση της σχολικής δέσμευσης όσο και της ικανοποίησης από το σχολικό πλαίσιο.
YOUTUBE E A PRODUÇÃO DAS INFÂNCIAS:
Authors: Salazar Guizzo Bianca 
Published: September 2024

A concepção de infância tomou novos rumos no Brasil especialmente a partir do século XX quando mais intensamente passou-se a compreender as necessidades específicas e peculiares das crianças. Ao mesmo tempo, percebe-se a grande visibilidade que as crianças passaram a ter na contemporaneidade. Um destes espaços de visibilidade é o YouTube. Tomando este espaço como educativo, este artigo tem como objetivo analisar a partir de um canal do YouTube protagonizados por crianças quais representações de gênero, articuladas às infâncias, são veiculadas e propagadas. Metodologicamente foi realizada uma análise cultural a partir da perspectiva teórica dos Estudos Culturais pós-estruturalistas, valendo-se especialmente dos conceitos de representação e de pedagogias culturais. Os resultados apontaram que há reiterações no que diz respeito aos scripts de gênero, mas também há rupturas, já que meninos e meninas têm a possibilidade de realizar ações e utilizar linguagens que são associadas tanto às feminilidades, como às masculinidades.
Determinants of stroke among adult hypertensive patients on follow up in Addis Ababa public hospitals, Ethiopia: A case control study
Authors: Teshome Feyisa , Baye Bayisa Abdissa , Wili Wekashi , Ifa Meseret , Kune Guta 
Published: September 2024

Background
Stroke is a non-communicable disease that causes sudden global or focal neurological disorders. It is one of the major public causes of morbidity and mortality in low- and middle-income countries, including Ethiopia. Early identification of the determinants and prompt intervention remains critical to reduce morbidity and mortality from stroke.

Objective
The study aimed to identify determinants of stroke among adult hypertensive patients on follow up in Addis Ababa public hospitals, Ethiopia.

Methods
Unmatched case-control study design was conducted among 326(109 cases and 217 controls) study participants in Addis Ababa public hospitals from September 1–30, 2021. The cases were adult hypertensive patients who developed a stroke and the controls were adult hypertensive patients. The study participants were selected by consecutive sampling technique. Pretested structured interviewer assisted questionnaire and checklist were used to collect data. Data were entered into Epi data version 3.1; exported and analysed by SPSS version 23. All independent variables with p-value < 0.25 in the bi-variable logistic regression analysis were entered into multivariable logistic regression analysis. Finally, variables with p-value <0.05 were considered as determinants of the stroke.

Results
In this study, current cigarette smoker(AOR = 5.55, 95% CI: 2.48, 12.43), current alcohol drinker(AOR = 4.27, 95% CI: 1.94, 9.38), medication non-compliance(AOR = 3.23, 95% CI: 1.62, 6.44), uncontrolled systolic blood pressure (AOR = 3.42, 95% CI: 1.64, 7.16), uncontrolled diastolic blood pressure(AOR = 4.29, 95% CI: 2.06, 8.93), high low density lipoprotein(AOR = 6.89, 95% CI: 3.57, 13.35) and diabetic mellitus(AOR = 3.25, 95% CI: 1.58, 6.69) were more likely to develop a stroke.

Conclusion and recommendation
Cigarette smoking, alcohol use, non-adherence to medication, uncontrolled systolic pressure, uncontrolled diastolic blood pressure, high low-density lipoprotein, and diabetic mellitus were determinants of stroke. Providing health education about lifestyle changes and the consequences of hypertension at all follow-up is very important.
ENTRE CURADORAS/ES E CURANDEIRAS/OS
Authors: Soares Mayara Ruth Nishiyama , Miranda Luciana Lobo , Oliveira Marta Clarice Nascimento , Sousa Bruna Ribeiro de 
Published: September 2024

Este relato parte de uma pesquisa de mestrado que discutiu como jovens pesquisadoras/es enunciavam sobre as questões de gênero que atravessavam seu cotidiano escolar. Assim, a partir da criação de um grupo de pesquisa composto por secundaristas de uma escola pública, enfocamos a articulação entre gênero e arte, e, com isso, germinamos a CURA(DOR)IA. Em interlocução com a Pesquisa-Inter(in)venção e a perspectiva do PesquisarCOM, o objetivo deste relato é discutir o dispositivo artístico CURA(DOR)IA, produzido como culminância de uma pesquisa participativa com jovens pesquisadores/as do seu cotidiano escolar em uma periferia de Fortaleza/CE. Destacamos duas cenas em que essas vivências são demarcadas, percebendo singularidades acerca do que a juventude daquele território traz com relação às experiências permeadas por essas discussões. Pontuamos o encontro como um dispositivo de abertura para produção de modos de vida mais inventivos, vislumbrando na diferença e na interface desta com a arte uma via a se seguir para pensar possíveis transgressões no debate.
Epidemiologi Perdarahan Saluran Cerna Pada Pasien Endoskopi di Rumah Sakit Umum Daerah Cut Meutia, Aceh Utara, propinsi Aceh
Authors: Ikhsan Maulana , Sayuti Muhammad , Ariyanda Iqbal , Akbar Teuku Ilhami Surya 
Published: September 2024

Background: Gastrointestinal bleeding is one of the main cause of death worldwide. For decades, the prevalence between lower gastrointestinal bleeding and upper gastrointestinal bleeding is constant. However, latest study showed that this may change due to the invention of new medication that prevent upper gastrointestinal bleeding. The epidemiology of gastrointestinal bleeding is important to plan the future promotion and prevention in the risk group and also to understand the distribution of the patient and their risk factor. The shortage of epidemiological data in developing countries makes the treatment of gastrointestinal bleeding became more challenging.Methods: This study is a retrospective study that aims to depict the epidemiology of gastrointestinal bleeding in patients who did gastroscopy or colonoscopy in Cut Meutia general hospital in North Aceh, Aceh Province. We analyze the medical record of the required patients in the interval of January 2019 to August 2021.Result: The incidence of lower gastrointestinal bleeding is higher than upper gastrointestinal bleeding. Female patients are dominant in develop gastrointestinal bleeding and elderly is the most common age that develop gastrointestinal bleeding.Conclusion : In general, the epidemiology of gastrointestinal bleeding in Cut Meutia general Hospital in North Aceh shares the same tendency with the incidence of gastrointestinal bleeding in many countries in the world, but there are several finding that differ such as high incidency of gastritic tumor and high incidency of gastritic tumor in younger age in male.
Transformación del derecho a decidir: análisis a la despenalización del delito de aborto en Colombia y derechos de la mujer
Authors: Sierra Zamora Paola Alexandra , Daza Gonzalez Alfonso , Daza Lora Santiago Andrés 
Published: September 2024

Se estudia en este artículo la despenalización del delito de aborto en el Código Penal colombiano desde un enfoque de derechos humanos. Este análisis conlleva que se califique el aborto como derecho fundamental de la mujer. En cuanto a los métodos de investigación, se emplea un enfoque cualitativo, basado en el análisis de contenido de fuentes primarias y secundarias, recopilando y examinando críticamente la normativa legal, los fallos judiciales y la literatura académica pertinente para fundamentar su argumentación a favor de la despenalización del aborto. Lo anterior se centra en la construcción de un marco teórico-jurídico que sitúe el debate en torno al aborto en el contexto de los derechos de la mujer. La pregunta que se pretende resolver es: ¿cuál es el impacto de la jurisprudencia constitucional e internacional en la protección de los derechos de las mujeres en relación con el aborto en Colombia?
The Limits of Plato’s Test
Authors: Meadows Katherine 
Published: September 2024

Abstract
Aristotle is often taken to define priority in being in Metaphysics Δ.11, where he says that those things are prior in being which “admit of being without other things, while these others cannot be without them: a division which Plato used” (1019a3-4). But Aristotle’s pattern of arguments about priority – some of which use Plato’s Test and others of which use distinct, causal tests – looks puzzling if Plato’s Test is his definition. This paper offers a new interpretation of Δ.11 on which it offers a guide to the Aristotelian use of Plato’s Test, given that being is said in many ways, rather than endorsing it as Aristotle’s own definition of priority. This reading illuminates Aristotle’s pattern of arguments in the corpus – explaining why Aristotle uses Plato’s Test in some cases and departs from it in others – and suggests a plausible story about how these arguments were shaped by engaging with Plato.
Knockdown of HCK promotes HREC cell viability and inner blood–retinal barrier integrity by regulating the AMPK signaling pathway
Authors: Chen Lu , Lin Chengmin 
Published: September 2024

Abstract
Diabetic retinopathy (DR), a major complication of diabetes causing blindness, is characterized by retinal damage due to capillary degeneration and vascular leakage. Current treatments are not fully effective, highlighting the need for searching new therapeutic targets. Hematopoietic cell kinase (HCK), a protein involved in various diseases, has been identified as a potential biomarker in DR, but its role in disease progression requires further investigation. Here we investigated the role of HCK in DR and its potential mechanism. We found the expression of HCK increased under the stimulation of high glucose (HG) in human retinal capillary endothelial cells (HRECs). Knockdown of HCK can improve HREC cell viability and the integrity of the internal blood–retinal barrier. HCK depletion suppressed the AMPK pathway in HG-induced HRECs. In summary, HCK may be a potential target for the treatment of DR, which provides a theoretical basis for the development of new treatment strategies.
Antibacterial, antifungal, and phytochemical properties of Salsola kali ethanolic extract
Authors: Bashir Shimaa , Behiry Said , Al-Askar Abdulaziz A. , Kowalczewski Przemysław Łukasz , Emaish Haitham H. , Abdelkhalek Ahmed 
Published: September 2024

Abstract
The research into the use of plants as plentiful reservoirs of bioactive chemicals shows significant potential for agricultural uses. This study focused on analyzing the chemical composition and potency of an ethanolic extract obtained from the aerial parts (leaves and stems) of Salsola kali against potato pathogenic fungal and bacterial pathogens. The isolated fungal isolates were unequivocally identified as Fusarium oxysporum and Rhizoctonia solani based on morphological characteristics and internal transcribed spacer genetic sequencing data. The antifungal activity of the extract revealed good inhibition efficacy against R. solani (60.4%) and weak activity against F. oxysporum (11.1%) at a concentration of 5,000 µg/mL. The S. kali extract exhibited strong antibacterial activity, as evidenced by the significant inhibition zone diameter (mm) observed in all three strains of bacteria that were tested: Pectobacterium carotovorum (13.33), Pectobacterium atrosepticum (9.00), and Ralstonia solanacearum (9.33), at a concentration of 10,000 µg/mL. High-performance liquid chromatography analysis revealed the presence of several polyphenolic compounds (μg/g), with gallic acid (2942.8), caffeic acid (2110.2), cinnamic acid (1943.1), and chlorogenic acid (858.4) being the predominant ones. Quercetin and hesperetin were the predominant flavonoid components, with concentrations of 1110.3 and 1059.3 μg/g, respectively. Gas chromatography-mass spectrometry analysis revealed the presence of many bioactive compounds, such as saturated and unsaturated fatty acids, diterpenes, and phytosterols. The most abundant compound detected was n-hexadecanoic acid, which accounted for 28.1%. The results emphasize the potential of S. kali extract as a valuable source of bioactive substances that possess good antifungal and antibacterial effects, which highlights its potential for many agricultural uses.
Individual-based numerical experiment to describe the distribution of floating kelp within the Southern Benguela Upwelling System
Authors: Coppin Ross , Rautenbach Christo , Smit Albertus J. 
Published: September 2024

Abstract
Kelps are resilient organisms, capable of thriving in high-energy wave environments. However, when hydrodynamic drag forces exerted by the wave environment exceed the kelps’ structural limits, individuals become dislodged. Floating kelps generally follow ocean currents, traveling long distances until air-filled structures fail or the epibiont load becomes too great, causing them to sink to the seafloor. The ability of kelp to disperse over vast offshore and nearshore systems makes them important for organic subsidy and as a dispersal vector for marine organisms. Previous research on dislodged macroalgae focused on context-specific rafts, limiting insights into the broader ecological role of floating kelp. This study employed a site-specific Lagrangian trajectory model to describe the spatial distribution of floating Ecklonia maxima along the South African coastline. The model incorporated buoyancy and sinking using site-specific morphological data. Findings revealed that the distribution of floating E. maxima is influenced by oceanographic conditions, and seasonal patterns were also evident. Mesoscale features played a vital role in kelp accumulation on the surface and seafloor and acted as barriers to dispersal. This study offers essential insights into kelp’s role as an organic subsidy and provides numerical evidence for kelp’s potential as a carbon sink, contributing to a better understanding of kelp ecosystems and their ecological functions.
Static perfect fluid spacetime on Riemannian manifolds admitting concurrent-recurrent vector field with Bach tensor
Authors: Praveena M. M. , Kumara H. Aruna , Arjun C. M. , Siddesha M. S. 
Published: September 2024

Abstract
In this paper, we first consider the

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equation on a Riemannian CRVF-manifold M and show that either M is Einstein or the potential function is pointwise collinear with ζ on an open set U of M. Next, we show that if a Riemannian CRVF-manifold M is the spatial factor of a

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with a Batch tensor then it is a Batch flat space-time manifold.