bluuebunny/crossref_metadata_2025_split huggingface dataset:
Up‐regulation of miR‐455‐5p by the TGF‐β–SMAD signalling axis promotes the proliferation of oral squamous cancer cells by targeting UBE2B
Authors: Cheng Chao‐Min , Shiah Shine‐Gwo , Huang Chien‐Chang , Hsiao Jenn‐Ren , Chang Jang‐Yang 
Published: May 2016

AbstractMicroRNAs (miRNAs) are involved in the tumourigenesis of various cancers by regulating their downstream targets. To identify the changes of miRNAs in oral squamous cell carcinoma (OSCC), we investigated the expression profiles of miRNAs in 40 pairs of OSCC specimens and their matched non‐tumour epithelial tissues. Our data revealed higher miR‐455‐5p expression in the tumour tissues than in the normal tissues; the expression was also higher in oral cancer cell lines than in normal keratinocyte cell lines. MiR‐455‐5p knockdown reduced both the anchorage‐independent growth and the proliferative ability of oral cancer cells, and these factors increased in miR‐455‐5p‐overexpressing cells. Furthermore, by analysing the array data of patients with cancer and cell lines, we identified ubiquitin‐conjugating enzyme E2B (UBE2B) as a target of miR‐455‐5p, and further validated this using 3′‐untranslated region luciferase reporter assays and western blot analysis. We also demonstrated that UBE2B suppression rescued the impaired growth ability of miR‐455‐5p‐knockdown cells. Furthermore, we observed that miR‐455‐5p expression was regulated, at least in part, by the transforming growth factor‐β (TGF‐β) pathway through the binding of SMAD3 to specific promoter regions. Notably, miR‐455‐5p expression was associated with the nodal status, stage, and overall survival in our patients, suggesting that miR‐455‐5p is a potential marker for predicting the prognosis of patients with oral cancer. In conclusion, we reveal that miR‐455‐5p expression is regulated by the TGF‐β‐dependent pathway, which subsequently leads to UBE2B down‐regulation and contributes to oral cancer tumourigenesis. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Overexpression of RANKL in osteoblasts: a possible mechanism of susceptibility to bone disease in cystic fibrosis
Authors: Delion Martial , Braux Julien , Jourdain Marie‐Laure , Guillaume Christine , Bour Camille , Gangloff Sophie , Pimpec‐Barthes Françoise Le , Sermet‐Gaudelus Isabelle , Jacquot Jacky , Velard Frédéric 
Published: May 2016

AbstractBone fragility and loss are a significant cause of morbidity in patients with cystic fibrosis (CF), and the lack of effective therapeutic options means that treatment is more often palliative rather than curative. A deeper understanding of the pathogenesis of CF‐related bone disease (CFBD) is necessary to develop new therapies. Defective CF transmembrane conductance regulator (CFTR) protein and chronic inflammation in bone are important components of the CFBD development. The receptor activator of nuclear factor kappa‐B ligand (RANKL) and osteoprotegerin (OPG) drive the regulation of bone turnover. To investigate their roles in CFBD, we evaluated the involvement of defective CFTR in their production level in CF primary human osteoblasts with and without inflammatory stimulation, in the presence or not of pharmacological correctors of the CFTR. No major difference in cell ultrastructure was noted between cultured CF and non‐CF osteoblasts, but a delayed bone matrix mineralization was observed in CF osteoblasts. Strikingly, resting CF osteoblasts exhibited strong production of RANKL protein, which was highly localized at the cell membrane and was enhanced in TNF (TNF‐α) or IL‐17‐stimulated conditions. Under TNF stimulation, a defective response in OPG production was observed in CF osteoblasts in contrast to the elevated OPG production of non‐CF osteoblasts, leading to an elevated RANKL‐to‐OPG protein ratio in CF osteoblasts. Pharmacological inhibition of CFTR chloride channel conductance in non‐CF osteoblasts replicated both the decreased OPG production and the enhanced RANKL‐to‐OPG ratio. Interestingly, using CFTR correctors such as C18, we significantly reduced the production of RANKL by CF osteoblasts, in both resting and TNF‐stimulated conditions. In conclusion, the overexpression of RANKL and high membranous RANKL localization in osteoblasts are related to defective CFTR, and may worsen bone resorption, leading to bone loss in patients with CF. Targeting osteoblasts with CFTR correctors may represent an effective strategy to treat CFBD. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Experimentally‐induced anti‐myeloperoxidase vasculitis does not require properdin, MASP‐2 or bone marrow‐derived C5
Authors: Freeley Simon J , Popat Reena J , Parmar Kiran , Kolev Martin , Hunt Beverley J , Stover Cordula M , Schwaeble Willhelm , Kemper Claudia , Robson Michael G 
Published: May 2016

AbstractAnti‐neutrophil cytoplasmic antibody vasculitis is a systemic autoimmune disease with glomerulonephritis and pulmonary haemorrhage as major clinical manifestations. The name reflects the presence of autoantibodies to myeloperoxidase and proteinase‐3, which bind to both neutrophils and monocytes. Evidence of the pathogenicity of these autoantibodies is provided by the observation that injection of anti‐myeloperoxidase antibodies into mice causes a pauci‐immune focal segmental necrotizing glomerulonephritis which is histologically similar to the changes seen on renal biopsy in patients. Previous studies in this model have implicated the alternative pathway of complement activation and the anaphylatoxin C5a. Despite this progress, the factors that initiate complement activation have not been defined. In addition, the relative importance of bone marrow‐derived and circulating C5 is not known. This is of interest given the recently identified roles for complement within leukocytes. We induced anti‐myeloperoxidase vasculitis in mice and confirmed a role for complement activation by demonstrating protection in C3‐deficient mice. We showed that neither MASP‐2‐ nor properdin‐deficient mice were protected, suggesting that alternative pathway activation does not require properdin or the lectin pathway. We induced disease in bone marrow chimaeric mice and found that circulating and not bone marrow‐derived C5 was required for disease. We have therefore excluded properdin and the lectin pathway as initiators of complement activation and this means that future work should be directed at other potential factors within diseased tissue. In addition, in view of our finding that circulating and not bone marrow‐derived C5 mediates disease, therapies that decrease hepatic C5 secretion may be considered as an alternative to those that target C5 and C5a. © 2016 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Influence of a long‐side‐chain‐containing reactive diluent on the structure and mechanical properties of UV‐cured films
Authors: Shen Lu , Wang Ying , Zhao Qiang , Luo Fubin , Chen Junhua , Lu Mangeng , Liang Liyan , Wu Kun , Shi Jun 
Published: May 2016

AbstractThe dimethacrylate reactive diluent (HEMA‐DDSA), a long‐side‐chain‐containing reactive diluent, was prepared by reacting 2‐dodecen‐1‐ylsuccinic anhydride with two equivalents of hydroxyethyl methacrylate. Its structure was characterized by IR and 1H NMR spectroscopy. This new diluent was added into the formulation of UV‐curable epoxy acrylate networks. Results show that the formulation with the addition of HEMA‐DDSA has massively reduced viscosity and shows several attractive properties of epoxy acrylate oligomers. The mechanical resistance of the films is dramatically enhanced with the incorporation of long alkyl groups derived from HEMA‐DDSA, the plastic deformation zone expands, thus decreasing the inner stress of the polymer structure. Moreover, the cured coatings have a higher glass transition temperature as the percentage of HEMA‐DDSA is increased up to 5 wt%. Due to the excellent integrated performance of the polymeric films, HEMA‐DDSA proved to be an effective reactive diluent, which is of potential interest for applications in high performance materials. © 2016 Society of Chemical Industry
Plasticization effects of epoxidized vegetable oils on mechanical properties of poly(3‐hydroxybutyrate)
Authors: Garcia‐Garcia Daniel , Ferri Jose M , Montanes Nestor , Lopez‐Martinez Juan , Balart Rafael 
Published: May 2016

AbstractThe effect of various epoxidized vegetable oils as potential plasticizers for poly(3‐hydroxybutyrate) (PHB) was evaluated in terms of changes in mechanical and thermal properties and morphology. PHB is a biodegradable aliphatic polyester obtained from bacterial fermentation. High stiffness and fragility are two of its main drawbacks. To overcome this behaviour, PHB was plasticized with various amounts of two different epoxidized vegetable oils: epoxidized linseed oil (ELO) and epoxidized soybean oil (ESBO). The total ELO and ESBO content varied in the range 5 phr (per hundred resin) to 20 phr and plasticized PHB materials were obtained by melt extrusion and compounding followed by injection moulding. The results show that the plasticizing effect provided by ELO is more efficient than that by ESBO with balanced properties at a concentration of 10 phr ELO. ELO addition leads to an improvement in mechanical ductile properties with a noticeable increase in elongation at break and impact absorbed energy. With regard to thermal properties, the addition of both ELO and ESBO leads to a marked increase in thermal stability of PHB. All these findings suggest that addition of 10 phr ELO leads to optimized PHB formulations with potential uses in technical applications. © 2016 Society of Chemical Industry
Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line
Authors: Dehler Carola E. , Boudinot Pierre , Martin Samuel A. M. , Collet Bertrand 
Published: May 2016

Abstract
CRISPR/Cas9 system has been used widely in animals and plants to direct mutagenesis. To date, no such method exists for fish somatic cell lines. We describe an efficient procedure for genome editing in the Chinook salmon Oncorhynchus tshawytscha CHSE. This cell line was genetically modified to firstly overexpress a monomeric form of EGFP (cell line CHSE-E Geneticin resistant) and additionally to overexpress nCas9n, a nuclear version of Cas9 (cell line CHSE-EC, Hygromycin and Geneticin resistant). A pre-validated sgRNA was produced in vitro and used to transfect CHSE-EC cells. The EGFP gene was disrupted in 34.6 % of cells, as estimated by FACS and microscopy. The targeted locus was characterised by PCR amplification, cloning and sequencing of PCR products; inactivation of the EGFP gene by deletions in the expected site was validated in 25 % of clones. This method opens perspectives for functional genomic studies compatible with high-throughput screening.
An economic decision model for determining the appropriate level of business process standardization
Authors: Afflerbach Patrick , Bolsinger Manuel , Röglinger Maximilian 
Published: May 2016

AbstractBusiness process management (BPM) is an acknowledged source of corporate performance. A well-established element of the BPM toolbox by which organizations intend to tune the performance of their processes is business process standardization (BPS). So far, research on BPS has predominantly taken a descriptive perspective, analyzing how BPS affects different dimensions of process performance (e.g., cost, quality, time, flexibility). Only very few studies capitalize on the mature body of descriptive BPS knowledge to assist in determining an appropriate BPS level for an organization’s processes. Moreover, these studies do not resolve the BPS trade-off, i.e., the partly conflicting effects of BPS on process performance. To address this research problem, we propose a decision model that provides guidance on how to determine an economically appropriate BPS level for a business process. We thereby adopt the design science research (DSR) paradigm and draw from the body of knowledge on BPS as well as value-based management. We evaluated the decision model by discussing its design specification against theory-backed design objectives. We also validated the model’s applicability and usefulness in a real-world case where we applied the decision model and a prototypical implementation to the coverage switching processes of an insurance broker pool company. Finally, we challenged the decision model against the accepted evaluation criteria from the DSR literature.
Brief report: Changes in parent–adolescent joint activities between 2002 and 2014 in the Czech Republic, Health Behaviour in School‐aged Children (HBSC) study
Authors: Vokacova Jana , Badura Petr , Pavelka Jan , Kalman Michal , Hanus Radek 
Published: May 2016

ABSTRACTJoint family activities (JFA) are linked to healthy adolescent development. The aim of the present study is to report time trends in JFA between 2002 and 2014.The sample concerned 16 396 adolescents aged 11, 13, and 15 years (48.4% boys) from the 2002, 2006, 2010, and 2014 surveys of the Health Behaviour in School‐aged Children (HBSC) study in the Czech Republic. The overall changes in JFA were evaluated using logistic regression.Compared with 2002, there was a slight increase in four out of the six selected JFA in 2014. In particular, the likelihood of engaging in joint active activities (sports and walks) increased in the 2002–2014 period. Conversely, nowadays adolescents watch TV with their parents less frequently. Moreover, families today do not eat together as often as in 2002, which might have negative consequences for healthy adolescent development. Adolescents aged 11 get involved in JFA more than their older counterparts.
Cognitive variability—A marker for incident MCI and AD: An analysis for the Alzheimer's Disease Neuroimaging Initiative
Authors: Anderson Eric D. , Wahoske Michelle , Huber Mary , Norton Derek , Li Zhanhai , Koscik Rebecca L. , Umucu Emre , Johnson Sterling C. , Jones Jana , Asthana Sanjay , Gleason Carey E. ,   Alzheimer's Disease Neuroimaging Initiative
Published: May 2016

AbstractIntroductionThe potential of intra‐individual cognitive variability (IICV) to predict incident mild cognitive impairment (MCI) or Alzheimer's disease (AD) was examined and compared to well‐established neuroimaging and genetic predictors.MethodsIICV was estimated using four neuropsychological measures for n = 1324 Alzheimer's Disease Neuroimaging Initiative (ADNI) participants who were cognitively healthy or diagnosed with MCI at baseline. IICV was used to predict time to incident MCI or AD, and compared to hippocampal volume loss and APOE ε4 status via survival analysis.ResultsIn survival analyses, controlling for age, education, baseline diagonosis, and APOE ε4 status, likelihood ratio tests indicate that IICV is associated with time to cognitive status change in the full sample (P < .0001), and when the sample was restricted to individuals with MCI at baseline (P < .0001).DiscussionThese findings suggest IICV may be a low‐cost, noninvasive alternative to traditional AD biomarkers.
The frontier of RNA metamorphosis and ribosome signature in neocortical development
Authors: Kraushar Matthew L. , Popovitchenko Tatiana , Volk Nicole L. , Rasin Mladen‐Roko 
Published: May 2016

AbstractMore than a passive effector of gene expression, mRNA translation (protein synthesis) by the ribosome is a rapidly tunable and dynamic molecular mechanism. Neurodevelopmental disorders are associated with abnormalities in mRNA translation, protein synthesis, and neocortical development; yet, we know little about the molecular mechanisms underlying these abnormalities. Furthermore, our understanding of regulation of the ribosome and mRNA translation during normal brain development is only in its early stages. mRNA translation is emerging as a key driver of the rapid and timed regulation of spatiotemporal gene expression in the developing nervous system, including the neocortex. In this review, we focus on the regulatory role of the ribosome in neocortical development, and construct a current understanding of how ribosomal complex specificity may contribute to the development of the neocortex. We also present a microarray analysis of ribosomal protein‐coding mRNAs across the neurogenic phase of neocortical development, in addition to the dynamic enrichment of these mRNAs in actively translating neocortical polysomal ribosomes. Understanding the multivariate control of mRNA translation by ribosomal complex specificity will be critical to reveal the intricate mechanisms of normal brain development and pathologies of neurodevelopmental disorders.
Cognitive outcome in epileptic patients in a tertiary care centre in Kolkata, India
Authors: Das Gautam , Saha Piya , Mukhopadhyay Pritha , Saha Shankar 
Published: May 2016

Abstract
Objective The objective of the study is to assess the diverse cognitive dysfunctions in epileptic patients.
Methods Generalized tonic–clonic seizure (GTCS) and complex partial seizure (CPS) patients and those control matched and assessed for their IQ were undertaken for the present study. ANOVA test was used to assess the differences between GTCS and CPS with the control.
Results GTCS and CPS exhibit lower scores than the control. In number cancellation task, they are at par. GTCS performed poorer than CPS.
Conclusion Epileptic group showed impairment in working memory function, visuo-spatial skill, processing speed, visuo-perceptual attainment and reasoning ability. The deficit is more pervasive in GTCS group.
Cervical cancer screening programs and guidelines in low‐ and middle‐income countries
Authors: Olson Brody , Gribble Beth , Dias Jasmyni , Curryer Cassie , Vo Kha , Kowal Paul , Byles Julie 
Published: May 2016

AbstractBackgroundScreening reduces cervical cancer incidence and mortality.ObjectiveTo describe cervical cancer epidemiology and screening guidelines in six low‐ and middle‐income countries (LMICs) participating in the Study on global AGEing and adult health (SAGE).Search strategyIncidence, mortality, and screening‐rate data were obtained for six LMICs and three higher‐income comparator countries (Australia, USA, and UK). SCOPUS and PubMed were used to identify literature published after 2000 in English, using several screening‐linked terms.Selection CriteriaLiterature describing the use of cervical cancer screening guidelines in China, Ghana, India, Mexico, Russia, and South Africa were included.Data collection and analysisIncidence, mortality trends, and screening rates were graphed and screening recommendations were summarized.Main ResultsHigher rates of cervical cancer incidence, mortality, and 5‐year prevalence were found in LMICs compared with the comparator countries. LMICs with absent or newly implemented screening guidelines had the lowest rates of crude and effective cervical cancer screening, with high cancer incidence and mortality. Countries with established guidelines had higher screening rates and lower disease burden. Cost, inadequate knowledge, geographical location, and cultural views were common barriers to effective screening coverage.ConclusionWork must continue to improve the implementation of affordable, relevant, and achievable methods to improve screening coverage in LMICs.
Causes and avoidable factors in maternal death due to cesarean‐related hemorrhage in South Africa
Authors: Maswime Salome , Buchmann Eckhart 
Published: May 2016

AbstractObjectiveTo describe risk factors, clinical events, and avoidable factors in cases of maternal death due to bleeding during and after cesarean delivery.MethodsA retrospective study was undertaken of the clinical records of women who delivered in seven hospitals in Johannesburg, South Africa, between January 2013 and December 2014. Maternal deaths due to cesarean‐related hemorrhage during or within 42 days of cesarean delivery at 24 weeks or more were selected. Case records were audited using quantitative techniques to determine the events leading up to death.ResultsThere were 123 251 deliveries and 17 maternal deaths due to bleeding during or after cesarean (3.2 deaths per 10 000 deliveries). Risk factors included previous cesarean delivery, preoperative anemia, and placental abruption. Uterine atony and surgical trauma were the main causes of bleeding. Five (29%) women died before the cause of bleeding was found. Avoidable factors included delays in the recognition and management of shock. Thirteen (76%) women died within 48 hours of the cesarean procedure.ConclusionDeaths due to bleeding during and after cesarean have multifactorial causation. Maternal healthcare systems must be strengthened, with attention to the knowledge and skills of health workers. This requires increased clinical vigilance, a rapid effective response to obstetric hemorrhage and shock, and overall health system strengthening.
Microinfarcts are common and strongly related to dementia in the oldest‐old: The 90+ study
Authors: Corrada María M. , Sonnen Joshua A. , Kim Ronald C. , Kawas Claudia H. 
Published: May 2016

AbstractIntroductionWe estimated the prevalence of microinfarcts and their association with dementia in a cohort of oldest‐old participants.MethodsParticipants were from The 90+ Study, a population‐based study of people 90 years and older. Dementia diagnoses were assigned postmortem during a consensus conference. Microinfarcts were evaluated in six brain regions.ResultsAt death, the 213 participants were on average 97 years old, 69% were women, and 52% had dementia. Of the participants, 51% had microinfarcts and 17% had 3+ microinfarcts. The odds ratio (OR) for dementia was similar for 3+ microinfarcts (OR = 4.75, P < .01) and tangle stage V–VI (OR = 4.70, P < .001). Only microinfarcts in cortical regions (other than occipital) were associated to dementia.DiscussionIn this oldest‐old cohort, microinfarcts are common and contribute independently and similarly in magnitude to dementia as tangles. As risk factors for microinfarcts and other dementing pathologies are likely to differ, identifying these factors is crucial to developing prevention strategies for dementia in the oldest‐old.
Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus)
Authors: Tanideh Nader , Jamshidzadeh Akram , Ghanbari Saghesloo Ali , Rahmanifar Farhad , Mokhtari Maral , Koohi-Hosseinabadi Omid , Omidi Mahmood , Najibi Asma 
Published: May 2016

Abstract
Objective To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis.
Materials and methods Ulcerative colitis was induced in 80 rats those divided into 8 equal groups. Group 1 and 2 were negative controls receiving 1 mL/day of normal saline in enema and oral; group 3 and 4 as positive control 1 and 2 received 10 mg/kg of intra-colonic asacol and oral mesalazine; groups 5 and 6 received 20% and 40% of hydroalcoholic extract of Z. jujuba trans-rectally; group 7 and 8 received 1500 and 3000 mg/kg of hydroalcoholic extract of Z. jujuba orally, respectively. After 7 days, animals were evaluated for colon tissue histopathology, levels of malondialdehyde and IL-1β, and activities of superoxide dismutase, glutathione peroxidase and myeloperoxidase in colon tissue.
Results Hydroalcoholic extract of Z. jujuba in both forms of trans-rectal and oral administration especially in the higher doses could result into a more healing effect in damaged colonic tissue, more reduce glutathione peroxidase and IL-1β level. Also, these two doses (gel 40% and oral 3000 mg/kg) could more decrease the myeloperoxidase activity and stimulate superoxide dismutase and glutathione peroxidase activities. Also, gel 40% in transrectal administration was more potent than administration 3000 mg/kg in oral.
Conclusion The results of the present study indicated that Z. jujube may be considered as a treatment of choice for Ulcerative colitis especially in gel form and also in dose-dependent pattern.