bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoarchean tectonothermal imprints in the Rengali Province, eastern India and their implication on the growth of Singhbhum Craton: evidence from zircon U–Pb SHRIMP data
Authors: Bose S. , Das K. , Kimura K. , Hidaka H. , Dasgupta A. , Ghosh G. , Mukhopadhyay J. 
Published: May 2016

AbstractA detailed zircon U–Pb (SHRIMP) geochronological study of the amphibolite to granulite‐grade rocks of the Rengali Province of eastern India records the growth history of the southern margin of the Singhbhum Craton. Pelitic and mafic granulites from the gneissic belt exhibit contrasting styles of metamorphism. Zircon of the pelitic granulites from the eastern segment yields c. 3528–3064 Ma detrital ages. Charnockitic gneiss from the eastern segment has protolith age of 3058 ± 15 Ma while that from the central segment has protolith age of 2861 ± 30 Ma. The latter rock records high‐grade metamorphism at 2818 ± 15 Ma. Hornblende gneiss from the central sector has a protolith age of 2828 ± 9 Ma. Deformed leucogranite in the central and undeformed granitoid in the eastern segment were emplaced at 2807 ± 13 and 2809 ± 13 Ma respectively. The protolith of felsic gneiss from the central sector was emplaced at 2776 ± 24 Ma. Most of the zircon samples contain overgrowths of c. 2500 Ma, inferred to be the age of reworking of the gneissic belt. Our data suggest that the Rengali Province evolved as an orogenic belt in the Neoarchean time (c. 2800–2500 Ma) during southward growth of the Singhbhum Craton. These tectonothermal imprints at the margin of the Singhbhum Craton are possibly related to its inclusion within the supercontinent Ur.
Evaluation of anti‐acne property of purified bee venom serum in humans
Authors: Han Sang Mi , Pak Sok Cheon , Nicholls Young Mee , Macfarlane Nicola 
Published: May 2016

SummaryObjectiveAcne vulgaris is a chronic dermatologic disease with four factors involved in the development of lesions. Treatments need to address as many of these underlying factors as possible in order to reduce acne lesions. As such, purified bee venom (PBV™) serum is an attractive therapeutic option for acne, but little data exist on the efficacy of this treatment strategy.MethodsIn this prospective, noncomparative study, 30 subjects having mild‐to‐moderate acne vulgaris were enrolled and treated with PBV™ serum twice daily for a period of 6 weeks. Clinical evaluation of lesions by expert visual grading and image analysis were made at weeks 0 (baseline), 3, and 6.ResultsThe average visual acne grade of all volunteers significantly improved with the PBV™ serum treatment at weeks 3 (P < 0.05) and 6 (P < 0.001) when compared with the baseline grade at week 0. In addition, there was a mean percent improvement of 8.6% and 52.3% in acne grade observed after 3 and 6 weeks of PBV™ serum use, with 20% and 77% of the subjects showing improvement, respectively, when compared with baseline. Moreover, the subjects showed improvement in open comedones, closed comedones, papules, pustules, and nodules after 3 and 6 weeks of PBV™ serum use.ConclusionSix weeks of treatment with PBV™ serum was found to be effective in the treatment of mild‐to‐moderate acne vulgaris, with no incidence of serious side effects or irritation.
Who Borrows from the Lender of Last Resort?
Authors: DRECHSLER ITAMAR , DRECHSEL THOMAS , MARQUES‐IBANEZ DAVID , SCHNABL PHILIPP 
Published: May 2016

ABSTRACTWe analyze lender of last resort (LOLR) lending during the European sovereign debt crisis. Using a novel data set on all central bank lending and collateral, we show that weakly capitalized banks took out more LOLR loans and used riskier collateral than strongly capitalized banks. We also find that weakly capitalized banks used LOLR loans to buy risky assets such as distressed sovereign debt. This resulted in a reallocation of risky assets from strongly to weakly capitalized banks. Our findings cannot be explained by classical LOLR theory. Rather, they point to risk taking by banks, both independently and with the encouragement of governments, and highlight the benefit of unifying LOLR lending and bank supervision.
Prevalence of Cerebral Palsy among 8‐Year‐Old Children in 2010 and Preliminary Evidence of Trends in Its Relationship to Low Birthweight
Authors: Durkin Maureen S. , Benedict Ruth E. , Christensen Deborah , Dubois Lindsay A. , Fitzgerald Robert T. , Kirby Russell S. , Maenner Matthew J. , Van Naarden Braun Kim , Wingate Martha S. , Yeargin‐Allsopp Marshalyn 
Published: May 2016

AbstractBackgroundThe public health objective for cerebral palsy (CP) in the United States is to reduce the percentage of children with CP who were born low birthweight (LBW, <2500 g) by 10% between 2006 and 2020. This study reports the prevalence of CP in a constant surveillance area for the years 2006, 2008, and 2010 and describes initial progress towards the CP public health objective.MethodsData on children with CP at age 8 years were ascertained by the Autism and Developmental Disabilities Monitoring (ADDM) Network, a population‐based surveillance system that monitored CP in four areas of the United States.ResultsCP prevalence in 2010 was 2.9 per 1000 [95% confidence interval (CI) 2.6, 3.2], down from 3.5 (95% CI 3.2, 3.9) in the same surveillance area in 2006. Among CP cases with no documented postneonatal aetiology, 49.1% (95% CI 42.9, 55.2) were born LBW in 2010 compared with 54.3% (95% CI 48.4, 60.1) in 2006. In 2010, 28.1% (95% CI 22.9, 30.4) were born very low birthweight (VLBW, <1500 g) compared with 35.4% (95% CI 30.0, 41.2) in 2006. The relative risks for associations between CP and both LBW and VLBW also declined, though not significantly, during the study period.ConclusionsDeclines in the associations between CP and LBW categories may have contributed to declines during the study period in both the prevalence of CP and the percentage of children with CP who were born LBW or VLBW. Ongoing monitoring of these trends is warranted.
Resistance training‐induced changes in integrated myofibrillar protein synthesis are related to hypertrophy only after attenuation of muscle damage
Authors: Damas Felipe , Phillips Stuart M. , Libardi Cleiton A. , Vechin Felipe C. , Lixandrão Manoel E. , Jannig Paulo R. , Costa Luiz A. R. , Bacurau Aline V. , Snijders Tim , Parise Gianni , Tricoli Valmor , Roschel Hamilton , Ugrinowitsch Carlos 
Published: May 2016

Key points
Skeletal muscle hypertrophy is one of the main outcomes from resistance training (RT), but how it is modulated throughout training is still unknown.
We show that changes in myofibrillar protein synthesis (MyoPS) after an initial resistance exercise (RE) bout in the first week of RT (T1) were greater than those seen post‐RE at the third (T2) and tenth week (T3) of RT, with values being similar at T2 and T3.
Muscle damage (Z‐band streaming) was the highest during post‐RE recovery at T1, lower at T2 and minimal at T3.
When muscle damage was the highest, so was the integrated MyoPS (at T1), but neither were related to hypertrophy; however, integrated MyoPS at T2 and T3 were correlated with hypertrophy.
We conclude that muscle hypertrophy is the result of accumulated intermittent increases in MyoPS mainly after a progressive attenuation of muscle damage.
AbstractSkeletal muscle hypertrophy is one of the main outcomes of resistance training (RT), but how hypertrophy is modulated and the mechanisms regulating it are still unknown. To investigate how muscle hypertrophy is modulated through RT, we measured day‐to‐day integrated myofibrillar protein synthesis (MyoPS) using deuterium oxide and assessed muscle damage at the beginning (T1), at 3 weeks (T2) and at 10 weeks of RT (T3). Ten young men (27 (1) years, mean (SEM)) had muscle biopsies (vastus lateralis) taken to measure integrated MyoPS and muscle damage (Z‐band streaming and indirect parameters) before, and 24 h and 48 h post resistance exercise (post‐RE) at T1, T2 and T3. Fibre cross‐sectional area (fCSA) was evaluated using biopsies at T1, T2 and T3. Increases in fCSA were observed only at T3 (P = 0.017). Changes in MyoPS post‐RE at T1, T2 and T3 were greater at T1 (P < 0.03) than at T2 and T3 (similar values between T2 and T3). Muscle damage was the highest during post‐RE recovery at T1, attenuated at T2 and further attenuated at T3. The change in MyoPS post‐RE at both T2 and T3, but not at T1, was strongly correlated (r ≈ 0.9, P < 0.04) with muscle hypertrophy. Initial MyoPS response post‐RE in an RT programme is not directed to support muscle hypertrophy, coinciding with the greatest muscle damage. However, integrated MyoPS is quickly ‘refined’ by 3 weeks of RT, and is related to muscle hypertrophy. We conclude that muscle hypertrophy is the result of accumulated intermittent changes in MyoPS post‐RE in RT, which coincides with progressive attenuation of muscle damage.
Transcriptome Profiling of Antimicrobial Resistance in Pseudomonas aeruginosa
Authors: Khaledi Ariane , Schniederjans Monika , Pohl Sarah , Rainer Roman , Bodenhofer Ulrich , Xia Boyang , Klawonn Frank , Bruchmann Sebastian , Preusse Matthias , Eckweiler Denitsa , Dötsch Andreas , Häussler Susanne 
Published: May 2016

ABSTRACTEmerging resistance to antimicrobials and the lack of new antibiotic drug candidates underscore the need for optimization of current diagnostics and therapies to diminish the evolution and spread of multidrug resistance. As the antibiotic resistance status of a bacterial pathogen is defined by its genome, resistance profiling by applying next-generation sequencing (NGS) technologies may in the future accomplish pathogen identification, prompt initiation of targeted individualized treatment, and the implementation of optimized infection control measures. In this study, qualitative RNA sequencing was used to identify key genetic determinants of antibiotic resistance in 135 clinicalPseudomonas aeruginosaisolates from diverse geographic and infection site origins. By applying transcriptome-wide association studies, adaptive variations associated with resistance to the antibiotic classes fluoroquinolones, aminoglycosides, and β-lactams were identified. Besides potential novel biomarkers with a direct correlation to resistance, global patterns of phenotype-associated gene expression and sequence variations were identified by predictive machine learning approaches. Our research serves to establish genotype-based molecular diagnostic tools for the identification of the current resistance profiles of bacterial pathogens and paves the way for faster diagnostics for more efficient, targeted treatment strategies to also mitigate the future potential for resistance evolution.
Absence of the
cbb
3
Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in Rhodobacter sphaeroides
Authors: Chen Guangyu E. , Canniffe Daniel P. , Martin Elizabeth C. , Hunter C. Neil 
Published: May 2016

ABSTRACT

The characteristic green color associated with chlorophyll pigments results from the formation of an isocyclic fifth ring on the tetrapyrrole macrocycle during the biosynthesis of these important molecules. This reaction is catalyzed by two unrelated cyclase enzymes employing different chemistries. Oxygenic phototrophs such as plants and cyanobacteria utilize an oxygen-dependent enzyme, the major component of which is a diiron protein named AcsF, while BchE, an oxygen-sensitive [4Fe-4S] cluster protein, dominates in phototrophs inhabiting anoxic environments, such as the purple phototrophic bacterium
Rhodobacter sphaeroides
. We identify a potential
acsF
in this organism and assay for activity of the encoded protein in a strain lacking
bchE
under various aeration regimes. Initially, cells lacking
bchE
did not demonstrate AcsF activity under any condition tested. However, on removal of a gene encoding a subunit of the
cbb
3
-type respiratory terminal oxidase, cells cultured under regimes ranging from oxic to micro-oxic exhibited cyclase activity, confirming the activity of the oxygen-dependent enzyme in this model organism. Potential reasons for the utilization of an oxygen-dependent enzyme in anoxygenic phototrophs are discussed.

IMPORTANCE
The formation of the E ring of bacteriochlorophyll pigments is the least well characterized step in their biosynthesis, remaining enigmatic for over 60 years. Two unrelated enzymes catalyze this cyclization step; O
2
-dependent and O
2
-independent forms dominate in oxygenic and anoxygenic phototrophs, respectively. We uncover the activity of an O
2
-dependent enzyme in the anoxygenic purple phototrophic bacterium
Rhodobacter sphaeroides
, initially by inactivation of the high-affinity terminal respiratory oxidase, cytochrome
cbb
3
. We propose that the O
2
-dependent form allows for the biosynthesis of a low level of bacteriochlorophyll under oxic conditions, so that a rapid initiation of photosynthetic processes is possible for this bacterium upon a reduction of oxygen tension.
S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase
Authors: Rizza Salvatore , Montagna Costanza , Cardaci Simone , Maiani Emiliano , Di Giacomo Giuseppina , Sanchez-Quiles Virginia , Blagoev Blagoy , Rasola Andrea , De Zio Daniela , Stamler Jonathan S. , Cecconi Francesco , Filomeni Giuseppe 
Published: May 2016

Abstract
S-nitrosoglutathione reductase (GSNOR) represents the best-documented denitrosylase implicated in regulating the levels of proteins posttranslationally modified by nitric oxide on cysteine residues by S-nitrosylation. GSNOR controls a diverse array of physiologic functions, including cellular growth and differentiation, inflammation, and metabolism. Chromosomal deletion of GSNOR results in pathologic protein S-nitrosylation that is implicated in human hepatocellular carcinoma (HCC). Here we identify a metabolic hallmark of aberrant S-nitrosylation in HCC and exploit it for therapeutic gain. We find that hepatocyte GSNOR deficiency is characterized by mitochondrial alteration and by marked increases in succinate dehydrogenase (SDH) levels and activity. We find that this depends on the selective S-nitrosylation of Cys501 in the mitochondrial chaperone TRAP1, which mediates its degradation. As a result, GSNOR-deficient cells and tumors are highly sensitive to SDH inhibition, namely to α-tocopheryl succinate, an SDH-targeting molecule that induced RIP1/PARP1-mediated necroptosis and inhibited tumor growth. Our work provides a specific molecular signature of aberrant S-nitrosylation in HCC, a novel molecular target in SDH, and a first-in-class therapy to treat the disease. Cancer Res; 76(14); 4170–82. ©2016 AACR.
Multiregion Whole-Exome Sequencing Uncovers the Genetic Evolution and Mutational Heterogeneity of Early-Stage Metastatic Melanoma
Authors: Harbst Katja , Lauss Martin , Cirenajwis Helena , Isaksson Karolin , Rosengren Frida , Törngren Therese , Kvist Anders , Johansson Maria C. , Vallon-Christersson Johan , Baldetorp Bo , Borg Åke , Olsson Håkan , Ingvar Christian , Carneiro Ana , Jönsson Göran 
Published: May 2016

Abstract
Cancer genome sequencing has shed light on the underlying genetic aberrations that drive tumorigenesis. However, current sequencing-based strategies, which focus on a single tumor biopsy, fail to take into account intratumoral heterogeneity. To address this challenge and elucidate the evolutionary history of melanoma, we performed whole-exome and transcriptome sequencing of 41 multiple melanoma biopsies from eight individual tumors. This approach revealed heterogeneous somatic mutations in the range of 3%–38% in individual tumors. Known mutations in melanoma drivers BRAF and NRAS were always ubiquitous events. Using RNA sequencing, we found that the majority of mutations were not expressed or were expressed at very low levels, and preferential expression of a particular mutated allele did not occur frequently. In addition, we found that the proportion of ultraviolet B (UVB) radiation-induced C&gt;T transitions differed significantly (P &lt; 0.001) between early and late mutation acquisition, suggesting that different mutational processes operate during the evolution of metastatic melanoma. Finally, clinical history reports revealed that patients harboring a high degree of mutational heterogeneity were associated with more aggressive disease progression. In conclusion, our multiregion tumor-sequencing approach highlights the genetic evolution and non-UVB mutational signatures associated with melanoma development and progression, and may provide a more comprehensive perspective of patient outcome. Cancer Res; 76(16); 4765–74. ©2016 AACR.
Can Precipitation and Temperature from Meteorological Reanalyses Be Used for Hydrological Modeling?
Authors: Essou Gilles R. C. , Sabarly Florent , Lucas-Picher Philippe , Brissette François , Poulin Annie 
Published: May 2016

AbstractThis paper investigates the potential of reanalyses as proxies of observed surface precipitation and temperature to force hydrological models. Three global atmospheric reanalyses (ERA-Interim, CFSR, and MERRA), one regional reanalysis (NARR), and one global meteorological forcing dataset obtained by bias-correcting ERA-Interim [Water and Global Change (WATCH) Forcing Data ERA-Interim (WFDEI)] were compared to one gridded observation database over the contiguous United States. Results showed that all temperature datasets were similar to the gridded observation over most of the United States. On the other hand, precipitation from all three global reanalyses was biased, especially in summer and winter in the southeastern United States. The regional reanalysis precipitation was closer to observations since it indirectly assimilates surface precipitation. The WFDEI dataset was generally less biased than the reanalysis datasets. All datasets were then used to force a global conceptual hydrological model on 370 watersheds of the Model Parameter Estimation Experiment (MOPEX) database. River flows were computed for each watershed, and results showed that the flows simulated using NARR and gridded observations forcings were very similar to the observed flows. The simulated flows forced by the global reanalysis datasets were also similar to the observations, except in the humid continental and subtropical climatic regions, where precipitation seasonality biases degraded river flow simulations. The WFDEI dataset led to better river flows than reanalysis in the humid continental and subtropical climatic regions but was no better than reanalysis—and sometimes worse—in other climatic zones. Overall, the results indicate that global reanalyses have good potential to be used as proxies to observations to force hydrological models, especially in regions with few weather stations.
Evidence that adenosine contributes to Leao’s spreading depression in vivo
Authors: Lindquist Britta E , Shuttleworth C William 
Published: May 2016

Leao’s spreading depression of cortical activity is a propagating silencing of neuronal activity resulting from spreading depolarization (SD). We evaluated the contributions of action potential (AP) failure and adenosine A1 receptor (A1R) activation to the depression of evoked and spontaneous electrocorticographic (ECoG) activity after SD in vivo, in anesthetized mice. We compared depression with SD-induced effects on AP-dependent transmission, and synaptic potentials in the transcallosal and thalamocortical pathways. After SD, APs recovered rapidly, within 1–2 min, as demonstrated by evoked activity in distant projection targets. Evoked corticocortical postsynaptic potentials recovered next, within ∼5 min. Spontaneous ECoG and evoked thalamocortical postsynaptic potentials recovered together, after ∼10–15 min. The duration of ECoG depression was shortened 20% by systemic (10 mg/kg) or focal (30 µM) administration of A1R competitive antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). ECoG depression was also shortened by focal application of exogenous adenosine deaminase (ADA; 100 U/mL), and conversely, was prolonged 50% by the non-competitive ADA inhibitor deoxycoformycin (DCF; 100 µM). We concluded that while initial depolarization block is brief, adenosine A1R activation, in part, contributes to the persistent secondary phase of Leao’s cortical spreading depression.
Hepatic arterial damage after transarterial chemoembolization for the treatment of hepatocellular carcinoma: comparison of drug-eluting bead and conventional chemoembolization in a retrospective controlled study
Authors: Lee Seungsoo , Kim Kyoung Min , Lee Shin Jae , Lee Kwang-Hun , Lee Do Yun , Kim Man Deuk , Kim Do Young , Kim Seung Up , Won Jong Yun 
Published: May 2016

Background Transarterial chemoembolization (TACE) of hepatocellular carcinoma (HCC) frequently causes feeding artery stenosis or occlusion that may interfere with repeated treatment. Purpose To investigate the incidence and predictors of hepatic arterial damage (HAD) after drug-eluting bead-TACE (DEB-TACE) in comparison with conventional TACE (Conv-TACE). Material and Methods We retrospectively analyzed 54 patients who underwent DEB-TACE for HCC as an initial treatment with follow-up angiography and 54 patients who underwent Conv-TACE using doxorubicin-lipiodol mixture and gelfoam particles for comparison. HAD was evaluated after a single session of TACE and graded as follows: grade I, no significant wall irregularity; grade II, overt stenosis; grade III, occlusion. Results The incidence of HAD was significantly higher in the DEB-TACE group than the Conv-TACE group when analyzed per branch (odds ratio [OR], 6.36; P < 0.001) and per patient (OR, 3.15; P = 0.005). For each HAD grade, the mean doxorubicin dose was greater in the DEB-TACE group than in the Conv-TACE group ( P < 0.001, P = 0.053, and P = 0.01 for grades I, II, and III, respectively). In multivariate analysis, risk factors of HAD included mean doxorubicin dose and selective embolization in the Conv-TACE group ( P = 0.03 and P < 0.001, respectively) and mean doxorubicin dose in the DEB-TACE group ( P = 0.004). Conclusion The incidence and grade of HAD were higher after DEB-TACE compared to Conv-TACE with doxorubicin dose as a possible risk factor. HAD was independent of overall survival in both groups.
Role of Serum cartilage oligomeric matrix protein (COMP) in the diagnosis of rheumatoid arthritis (RA): A case–control study
Authors: Liu Fengxia , Wang Xijuan , Zhang Xude , Ren Cuiai , Xin Jie 
Published: May 2016

Objective To analyse the role of serum cartilage oligomeric matrix protein (COMP) levels in the differential diagnosis of rheumatoid arthritis (RA). Methods This case–control study analysed the clinical and laboratory characteristics of patients with RA and healthy control subjects. The diagnostic ability of COMP for RA was evaluated by comparing it with anti-cyclic citrullinated peptide antibody levels. The sensitivity, specificity, positive and negative predictive values were calculated. Results The study enrolled 82 patients with RA and 34 healthy control subjects. The serum COMP level was significantly higher in patients with RA compared with control subjects (mean ± SD 29.51 ± 9.21 ng/ml versus 17.85 ± 5.55 ng/ml, respectively). The serum COMP level was significantly higher in patients with active RA compared with patients with RA in remission (mean ± SD 33.08 ± 8.80 ng/ml versus 24.94 ± 7.65 ng/ml, respectively). The cut-off value for COMP to discriminate patients with RA from healthy individuals was 21.51 ng/ml (sensitivity 0.817, specificity 0.882, positive predictive value 0.944, negative predictive value 0.667, and accuracy 0.836). Conclusion The serum COMP level has the potential to be used as a biological marker for differentiating between patients with RA and healthy individuals.