bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leadership and Management Competencies of Nurse Managers: A Cross-sectional Study in Northern and Central Vietnam
Authors: Bui Thuy Thi , Nguyen An Thi Binh , Nhung Thi Trang Nguyen  , Cao Tung Viet , Truong Anh Tuan , Tran Dien Minh 
Published: September 2024

Objective: The competence of nurse managers is essential to ensure outstanding patient care and the key contributors to the healthcare organization’s success. This study aimed to adapt the Chase Nurse Manager Competency Instrument (CNMCI) to Vietnamese situations and then to examine the leadership, management, and related characteristics of nurse managers. Material and Methods: A cross-sectional study was conducted in 17 Children and Obstetric and Pediatric hospitals in northern and central area of Vietnam using the CNMCI. Nurse managers (n=227) who were working in these hospitals participated in this study. Data were collected from July 2022 to August 2022. Results: Nurse managers had 3.51 ± 0.42 level of knowledge and 2.95 ± 0.48 level of ability to apply leadership and management competencies. However, only level of hospital and number of employees were significant related factors of leadership and management competencies of nurse manager. Conclusion: This study was the first in Vietnam which we examined the leadership competencies of nurse managers in Obstetric and Pediatric Hospitals. The results suggested that Vietnamese version of CNMCI was a reliable, valid instrument to assess competencies of nurse managers. It is necessary to have an effective leadership and manager training program to improve the competencies of nurse managers.
Improving Hypertension Outcomes: Assessing Caregivers’ Knowledge, Attitude and Practices in Rural Malawi
Authors: Maluwa Mr. Chikondi , Kapira Mr. Sitalire , Chuljerm Dr. Hataichanok , Parklak Dr. Wason , Kulprachakarn Dr. Kanokwan 
Published: September 2024

Introduction: Hypertension is a major global health issue, significantly contributing to the burden of cardiovascular diseases, particularly in low- and middle-income countries (LMICs) like Malawi. Caregivers play a crucial role in managing hypertension in these settings, yet their knowledge, attitudes, and practices (KAP) are not well-documented.
Objectives: This study aimed to assess the knowledge, attitudes, and practices (KAP) of caregivers for hypertensive patients in rural Malawi, as well as to identify any disparities in these areas.
Methods: A descriptive cross-sectional study was conducted from November 2023 to February 2024 at Lisungwi Community Hospital and Neno District Hospital in Neno, Malawi. A total of 422 caregivers were conveniently sampled, with 248 from Lisungwi and 174 from Neno. Data were collected using a validated pre-tested questionnaire, assessing KAP on hypertension. Descriptive, one-way ANOVA and linear regression statistical analyses were performed using SPSS version 22.0.
Results: In both Lisungwi and Neno hospitals, the majority of participants were female, accounting for 63.7% and 62.6%, respectively. The mean age of participants was 44.7 years (± 10.3) in Lisungwi and 45.3 years (± 9.3) in Neno, with average care durations of 4.1 years (± 3.3) and 4.2 years (± 3.2). Notably, 94.4% of caregivers at Lisungwi and 94.3% at Neno exhibited poor knowledge of hypertension; however, all caregivers maintained a positive attitude toward its prevention and management. Significant differences in practice scores were observed (p < 0.001), with caregivers in Lisungwi performing better. Additionally, factors such as education, age, and occupation influenced Knowledge, Attitude, and Practices (KAP) scores, while gender impacted practices alone.
Conclusions: The findings of poor knowledge highlight the urgent need for enhanced educational interventions, gender-specific support, community engagement, and a context-specific approach to improve the knowledge and practices of caregivers, which are critical for effective hypertension prevention and management in rural setting.
Effect of Commercial Cationic Starch on Paper Properties Made from Recycled Pulp
Authors: Boon Jia Geng , Liew Jing Xian , Liew Jeng Young 
Published: September 2024

The production of packaging paper is growing steadily annually globally. Packaging paper such as liner paper and medium paper are mainly made from recycled pulp. The strength of pulp deteriorated over the cycle of recycling. To ensure the packaging paper strength produced from recycled paper is up to par, the additive is mandatory. Starch is one of the common and economical additives used in papermaking. In this research, the performance of commercial cationic starch as an additive in packaging papermaking is evaluated. 4 different percentages of starch, including 2%, 4%, 6%, and 8%, were studied. Five replicates of 60gsm hand sheets were produced for each level, and 0% was introduced as a control. The properties such as thickness, Canadian Standard Freeness Test, Cobb test, tensile index, folding endurance, and tear index were evaluated according to the TAPPI Standard. From the result, an 8% dosage of this commercial cationic starch exhibited the best performance in mechanical tests without compromising the drainage of water significantly.
Evolving Brains, Emerging Gods
Authors: Torrey E. Fuller 
Published: September 2024

Religions and mythologies from around the world teach that God or gods created humans. Atheist, humanist, and materialist critics, meanwhile, have attempted to turn theology on its head, claiming that religion is a human invention. In this book, E. Fuller Torrey draws on cutting-edge neuroscience research to propose a startling answer to the ultimate question. Evolving Brains, Emerging Gods locates the origin of gods within the human brain, arguing that religious belief is a by-product of evolution. Based on an idea originally proposed by Charles Darwin, Torrey marshals evidence that the emergence of gods was an incidental consequence of several evolutionary factors. Using data ranging from ancient skulls and artifacts to brain imaging, primatology, and child development studies, this book traces how new cognitive abilities gave rise to new behaviors. For instance, autobiographical memory, the ability to project ourselves backward and forward in time, gave Homo sapiens a competitive advantage. However, it also led to comprehension of mortality, spurring belief in an alternative to death. Torrey details the neurobiological sequence that explains why the gods appeared when they did, connecting archaeological findings including clothing, art, farming, and urbanization to cognitive developments. This book does not dismiss belief but rather presents religious belief as an inevitable outcome of brain evolution. Providing clear and accessible explanations of evolutionary neuroscience, Evolving Brains, Emerging Gods will shed new light on the mechanics of our deepest mysteries.
Identification of non-model mammal species using the MinION DNA sequencer from Oxford Nanopore
Authors: Velasquez-Restrepo Sara , Corrales Orozco Mariana , Franco-Sierra Nicolás D. , Martínez-Cerón Juan M. , Díaz-Nieto Juan F. 
Published: September 2024

Background
The Neotropics harbors the largest species richness of the planet; however, even in well-studied groups, there are potentially hundreds of species that lack a formal description, and likewise, many already described taxa are difficult to identify using morphology. Specifically in small mammals, complex morphological diagnoses have been facilitated by the use of molecular data, particularly from mitochondrial sequences, to obtain accurate species identifications. Obtaining mitochondrial markers implies the use of PCR and specific primers, which are largely absent for non-model organisms. Oxford Nanopore Technologies (ONT) is a new alternative for sequencing the entire mitochondrial genome without the need for specific primers. Only a limited number of studies have employed exclusively ONT long-reads to assemble mitochondrial genomes, and few studies have yet evaluated the usefulness of such reads in multiple non-model organisms.

Methods
We implemented fieldwork to collect small mammals, including rodents, bats, and marsupials, in five localities in the northern extreme of the Cordillera Central of Colombia. DNA samples were sequenced using the MinION device and Flongle flow cells. Shotgun-sequenced data was used to reconstruct the mitochondrial genome of all the samples. In parallel, using a customized computational pipeline, species-level identifications were obtained based on sequencing raw reads (Whole Genome Sequencing). ONT-based identifications were corroborated using traditional morphological characters and phylogenetic analyses.

Results
A total of 24 individuals from 18 species were collected, morphologically identified, and deposited in the biological collection of Universidad EAFIT. Our different computational pipelines were able to reconstruct mitochondrial genomes from exclusively ONT reads. We obtained three new mitochondrial genomes and eight new molecular mitochondrial sequences for six species. Our species identification pipeline was able to obtain accurate species identifications for up to 75% of the individuals in as little as 5 s. Finally, our phylogenetic analyses corroborated the identifications from our automated species identification pipeline and revealed important contributions to the knowledge of the diversity of Neotropical small mammals.

Discussion
This study was able to evaluate different pipelines to reconstruct mitochondrial genomes from non-model organisms, using exclusively ONT reads, benchmarking these protocols on a multi-species dataset. The proposed methodology can be applied by non-expert taxonomists and has the potential to be implemented in real-time, without the need to euthanize the organisms and under field conditions. Therefore, it stands as a relevant tool to help increase the available data for non-model organisms, and the rate at which researchers can characterize life specially in highly biodiverse places as the Neotropics.
Identification of a novel immune infiltration-related gene signature, MCEMP1, for coronary artery disease
Authors: Ye Wei , Shen Bo , Tang Qizhu , Fang Chengzhi , Wang Lei , Xie Lili , He Qi 
Published: September 2024

Background
This study aims to identify a novel gene signature for coronary artery disease (CAD), explore the role of immune cell infiltration in CAD pathogenesis, and assess the cell function of mast cell-expressed membrane protein 1 (MCEMP1) in human umbilical vein endothelial cells (HUVECs) treated with oxidized low-density lipoprotein (ox-LDL).

Methods
To identify differentially expressed genes (DEGs) of CAD, datasets GSE24519 and GSE61145 were downloaded from the Gene Expression Omnibus (GEO) database using the R “limma” package with p < 0.05 and |log2 FC| > 1. Gene ontology (GO) and pathway analyses were conducted to determine the biological functions of DEGs. Hub genes were identified using support vector machine-recursive feature elimination (SVM-RFE) and least absolute shrinkage and selection operator (LASSO). The expression levels of these hub genes in CAD were validated using the GSE113079 dataset. CIBERSORT program was used to quantify the proportion of immune cell infiltration. Western blot assay and qRT‐PCR were used to detect the expression of hub genes in ox-LDL-treated HUVECs to validate the bioinformatics results. Knockdown interference sequences for MCEMP1 were synthesized, and cell proliferation and apoptosis were examined using a CCK8 kit and Muse® Cell Analyzer, respectively. The concentrations of IL-1β, IL-6, and TNF-α were measured with respective enzyme-linked immunosorbent assay (ELISA) kits.

Results
A total of 73 DEGs (four down-regulated genes and 69 up-regulated genes) were identified in the metadata (GSE24519 and GSE61145) cohort. GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results indicated that these DEGs might be associated with the regulation of platelet aggregation, defense response or response to bacterium, NF-kappa B signaling pathway, and lipid and atherosclerosis. Using SVM-RFE and LASSO, seven hub genes were obtained from the metadata. The upregulated expression of DIRC2 and MCEMP1 in CAD was confirmed in the GSE113079 dataset and in ox-LDL-treated HUVECs. The associations between the two hub genes (DIRC2 and MCEMP1) and the 22 types of immune cell infiltrates in CAD were found. MCEMP1 knockdown accelerated cell proliferation and suppressed cell apoptosis for ox-LDL-treated HUVECs. Additionally, MCEMP1 knockdown appeared to decrease the expression of inflammatory factors IL-1β, IL-6, and TNF-α.

Conclusions
The results of this study indicate that MCEMP1 may play an important role in CAD pathophysiology.
HPV-driven heterogeneity in cervical cancer: study on the role of epithelial cells and myofibroblasts in the tumor progression based on single-cell RNA sequencing analysis
Authors: Zhang Yunyun , Zhang Yu , Pan Chenke , Wang Wenqian , Yu Yao 
Published: September 2024

Background
Cervical cancer (CC) is a neoplasia with a high heterogeneity. We aimed to explore the characteristics of tumor microenvironment (TME) for CC treatment.

Methods
HPV positive (+) and negative (−) samples from cervical cancer (CC) patients were sourced from the Gene Expression Omnibus (GEO) database. The single-cell RNA sequencing (scRNA-seq) data were processed and annotated for cell types utilizing the Seurat package. Following this, the expression levels and biological roles of the marker genes were analyzed applying real-time PCR (RT-PCR) and transwell assays. Furthermore, the enrichment of genes with significantly differential expressions and copy number variations was assessed by the ClusterProlifer and inferCNV software packages.

Results
Seven main cell clusters were classified based on a total of 12,431 cells. The HPV− CC samples exhibited a higher immune cell infiltration level, while epithelial cells and myofibroblasts had higher proportion in the HPV+ CC samples with extensive heterogeneity. Immune pathways including antigen treatment and presentation, immunoglobulin production and T cell mediated immunity were significantly activated in the HPV− CC group with lower cell cycle and proliferation activity. However, the anti-tumor immunity of these cells was inhibited in HPV+ CC group with higher cell proliferation activity. Moreover, the amplification and loss of CNVs also supported that these cells in HPV− CC samples were prone to anti-tumor activation. Further cell validation results showed that except GZMA, the levels of APOC1, CEACAM6, FOXP3, SFRP4 and TFF3 were all higher in CC cells Hela, and that silencing TFF3 could inhibit the migration and invasion of CC cells in-vitro.

Conclusion
This study highlighted the critical role of HPV infection in CC progression, providing a novel molecular basis for optimizing the current preventive screening and personalized treatment for the cancer.
Interrelationship between Non-Alcoholic Steatohepatitis and Vitamin D: A Mini Review
Authors: Kaintura Anil , Hussein Mohamed Abdelrahman El 
Published: September 2024

Non-alcoholic steatohepatitis (NASH) is a progressive liver disease characterized by inflammation and accumulation of fat in the liver, often associated with obesity, diabetes and metabolic syndrome leading to fibrosis and ultimately cirrhosis of the liver. The literature search included original articles, randomized controlled trials, cohort studies, case-control studies and review articles from the year 2004 onwards. The electronic databases Cochrane, MEDLINE, ScienceDirect and PubMed were used for the literature search. The current state of evidence shows a positive correlation between non-alcoholic steatohepatitis and vitamin D deficiency. The association between vitamin D and non-alcoholic steatohepatitis (NASH) is a complex one and further studies are needed for understanding this relationship. This review is aimed at the relationship between non-alcoholic steatohepatitis and vitamin D, additionally, it will discuss the role of vitamin D in modulating immune responses with its protective effects on the liver. The need for maintaining an adequate level of vitamin D in at-risk populations will also be discussed along with future research in this matter.
Alpinia zerumbet Anxiolytic and Antidepressant Effects: A Literature Review
Authors: Ferreira Flávia dos Santos , Silva Paula Hosana Fernandes da , Mattos Juliana Lucena Azevedo de , Resende Angela de Castro , Ognibene Dayane Teixeira , Costa Cristiane Aguiar da , Montes Guilherme Carneiro , Fontes-Dantas Fabrícia Lima , Bem Graziele Freitas de 
Published: September 2024

Studies demonstrated that many elderly individuals suffer from depressive and anxiety disorders. The drugs used to treat these disorders cause adverse effects such as sedation, gastrointestinal discomfort, and locomotor deficits, which are often responsible for treatment abandonment. Literature data also describe that older people may be refractory to pharmacological treatments used to combat depressive and anxiety disorders, which is potentially worrying due to the suicide risk associated with these conditions. Previous research reported the medicinal plant's beneficial potential in psychiatric disease treatment due to its antioxidant, anti-inflammatory, and antiapoptotic actions. This paper reviews the Alpinia zerumbet actions, a medicinal plant with antidepressant and anxiolytic effects, through increases in noradrenergic neurotransmission and decreases oxidative stress on the central nervous system by increasing antioxidant activity. Moreover, Alpinia zerumbet also causes a reduction in IL-6 and elevates BDNF levels in the hippocampus, contributing to its antidepressant, anxiolytic, and anti-inflammatory actions. The adverse effects reduction, combined with the fact that there is no toxicity when ingested by humans and increased longevity, suggests that this medicinal plant is promising for older individuals treatment who suffer from depression and anxiety.
Nucleation process of magnitude 2 repeating earthquakes on the San Andreas Fault predicted by rate‐and‐state fault models with SAFOD drill core data
Authors: Kaneko Yoshihiro , Carpenter Brett M. , Nielsen Stefan B. 
Published: December 2016

AbstractRecent laboratory shear‐slip experiments conducted on a nominally flat frictional interface reported the intriguing details of a two‐phase nucleation of stick‐slip motion that precedes the dynamic rupture propagation. This behavior was subsequently reproduced by a physics‐based model incorporating laboratory‐derived rate‐and‐state friction laws. However, applying the laboratory and theoretical results to the nucleation of crustal earthquakes remains challenging due to poorly constrained physical and friction properties of fault zone rocks at seismogenic depths. Here we apply the same physics‐based model to simulate the nucleation process of crustal earthquakes using unique data acquired during the San Andreas Fault Observatory at Depth (SAFOD) experiment and new and existing measurements of friction properties of SAFOD drill core samples. Using this well‐constrained model, we predict what the nucleation phase will look like for magnitude ∼2 repeating earthquakes on segments of the San Andreas Fault at a 2.8 km depth. We find that despite up to 3 orders of magnitude difference in the physical and friction parameters and stress conditions, the behavior of the modeled nucleation is qualitatively similar to that of laboratory earthquakes, with the nucleation consisting of two distinct phases. Our results further suggest that precursory slow slip associated with the earthquake nucleation phase may be observable in the hours before the occurrence of the magnitude ∼2 earthquakes by strain measurements close (a few hundred meters) to the hypocenter, in a position reached by the existing borehole.
Extensive and drastically different alpine lake changes on Asia's high plateaus during the past four decades
Authors: Zhang Guoqing , Yao Tandong , Piao Shilong , Bolch Tobias , Xie Hongjie , Chen Deliang , Gao Yanhong , O'Reilly Catherine M. , Shum C. K. , Yang Kun , Yi Shuang , Lei Yanbin , Wang Weicai , He You , Shang Kun , Yang Xiankun , Zhang Hongbo 
Published: December 2016

AbstractAsia's high plateaus are sensitive to climate change and have been experiencing rapid warming over the past few decades. We found 99 new lakes and extensive lake expansion on the Tibetan Plateau during the last four decades, 1970–2013, due to increased precipitation and cryospheric contributions to its water balance. This contrasts with disappearing lakes and drastic shrinkage of lake areas on the adjacent Mongolian Plateau: 208 lakes disappeared, and 75% of the remaining lakes have shrunk. We detected a statistically significant coincidental timing of lake area changes in both plateaus, associated with the climate regime shift that occurred during 1997/1998. This distinct change in 1997/1998 is thought to be driven by large‐scale atmospheric circulation changes in response to climate warming. Our findings reveal that these two adjacent plateaus have been changing in opposite directions in response to climate change. These findings shed light on the complex role of the regional climate and water cycles and provide useful information for ecological and water resource planning in these fragile landscapes.
Applications of the Jupiter Global Ionosphere‐Thermosphere Model: A case study of auroral electron energy deposition
Authors: Egert Austin , Waite J. Hunter , Bell Jared 
Published: December 2016

AbstractWe investigate auroral energy deposition by using a nonhydrostatic global atmospheric model coupled to a two‐stream electron transport model. We present several electron beam study cases, discussing energy flux and electron energy effects on the ion and neutral densities, the atmospheric thermal profile, H3+ and hydrocarbon infrared (IR) emissions, H2 far ultraviolet (FUV) emissions and color ratios, and vibrationally excited molecular hydrogen. Using the nonhydrostatic Jupiter Global Ionosphere‐Thermosphere Model, we find that FUV spectral characteristics consistent with previous Hubble Space Telescope results derive primarily from electrons with energies above 10 keV, over energy fluxes of 10–100 erg/cm2 s, while IR emissions are predominantly due to electrons with energies below 10 keV, over energy fluxes of 10–100 erg/cm2 s. Electrons with energies below about 10 keV produce enough H2(ν) to deplete the H+ population, modifying the ionospheric composition, and consequently the H3+ emissions, which can be used to directly relate H2 vibrational excitation to auroral observations. New observations by Juno will provide better electron energy distributions to constrain the electron energy spectrum and magnitude at the upper boundary of the model and simultaneously provide a determination of the FUV and IR spectra that can be cross‐correlated with the observations.