bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of the personality traits of newly graduated nurses on the knowledge, skills and professional self-efficacy in standardized training: a cross-sectional study
Authors: Yang Jie , Mao Tianyang , Yuan Ping , Zhou Juan , Li Mengqun , Chen Bing 
Published: September 2024

Abstract

Background
New graduate nurses often have certain potential safety hazards for patients due to insufficient knowledge and lack of clinical practice ability. To address these challenges, China has implemented a standardized training strategy. The improvements in the quality of this training involve not only the intervention of teaching methods but also the consideration of personality traits.
Methods
The application software based on the BFI-2 Chinese scale was utilized to administer personality tests to nursing students; nursing students were invited to scan the QR code and voluntarily fill in a questionnaire, including basic information, personality test results, and a professional self-efficacy test scale; offline paper-based theoretical examination results of nursing students were collected before and after training. The data was then analyzed using SPSS software version 26.0, which involved descriptive analysis, one-way between-groups analysis of variance (ANOVA) and Spearman correlation analysis.
Results
Based on the data, there were no observable differences in the theoretical results before and after training across different personality traits. In terms of skill assessment, conscientiousness exhibited the highest score at 78.91±2.98 points, while negative emotionality showed the lowest score at 74.59±2.12 points. These differences between different personality traits are statistically significant (P<0.001). In terms of professional self-efficacy, conscientiousness scored the highest at 98.48±12.69, while negative emotionality scored the lowest at 85.89±11.71, with significant differences between different personality traits (P<0.001). Professional self-efficacy was negatively correlated with agreeableness (r=-0.150, P=0.044) and positively correlated with conscientiousness (r=-0.310, P<0.001). Skill scores were negatively correlated with negative emotionality (r=-0.257, P<0.001) and positively correlated with conscientiousness (r=0.182, P=0.014).
Conclusions
This study shows that personality traits affect the skills test results and professional self-efficacy of nursing students. Conscientiousness scored the highest in this study, while negative emotionality scored the lowest. Therefore, personalized training plans are recommended to improve the quality of care for such nursing students and to further enhance patient safety.
Design and in silico assessment of quinoxaline platinum complexes as ATPase competitive topoisomerase inhibitors
Authors: Pincher Daniel W.M. , Hickey Shane M. , Plush Sally E. 
Published: September 2024

Abstract

The topoisomerase TOP2 enzyme is reliant on ATP hydrolysis for DNA relegation, when the ATPase domain is competitively inhibited the enzymatic process is halted and at stable DNA lesion results. This leads to increased apoptotic processes. Metal based topoisomerase inhibitors offer a unique perspective due to their greater diversity of molecular geometries, electrostatic charge and valencies compared with organic based inhibitors. To this effect, a range of platinum (II) complexes were designed and assessed in silico for their ability to competitively inhibit the TOP2 ATPase domain. The ligands were derived from the lead compound XK469 with modifications made on the quinoxaline core which was truncated to include different amino acid groups coordinated to a variety of platinum ions and secondary ligands. Binding energies were calculated for the interaction of the ligands and their complexes with the TOP2 ATPase domain and compared to binding energies for XK469 and ATP with the TOP2 ATPase domain. Platinum coordination was found to be beneficial with the majority of complexes demonstrating a higher binding energy with the TOP2 ATPase domain compared with the lead XK469 compound with 3 complexes exhibiting higher binding energy than the target ATP molecule. The results support two design strategies to improve the affinity for XK469 derivatives for the TOP2 ATPase domain, the first being the coordination of large secondary ligands (2,2'‑bipyridine and 1,10-phenanthroline) in close proximity to the aromatic quinoxaline group, and the second being the coordination of smaller ethylenediamine secondary ligand to a hydrophilic terminus.
Tubeless Ureteroenteric Anastomosis in Robot-assisted Radical Cystectomy with Intracorporeal Urinary Diversion does not increase the risk of anastomotic stenosis or postoperative complications
Authors: Taylor Zachariah , Musallam Sami , Meyer Kayla , Elkhashab Iman , Thomas Brian , Snow Zachary , Zeltser Ilia 
Published: September 2024

Abstract

Objective
To demonstrate that omitting ureteral stent placement in robotic intracorporeal urinary diversion does not lead to increased risk of perioperative complications, namely ureteral strictures or anastomotic leaks.
Methods
We retrospectively reviewed the records of 68 consecutive patients who underwent robotic radical cystectomy with ileal conduit creation or orthotopic neobladder by a single surgeon between January 2020 and September 2023. Chronologically, the first cohort of patients had ureteral stents placed to bridge the ureteroenteric anastomosis and in the second cohort stenting was omitted.
Results
Cohort 1 consisted of 28 patients with surgeries performed between January 2020 and April 2021, while cohort 2 had 40 patients who underwent surgery from April 2021 to September 2023. The cohorts were well matched with regards to patient age, gender, ASA score and rate of neoadjuvant chemotherapy. The choice of urinary diversion was left to surgeon and patient preference and there was no significant difference in the proportion of ileal conduits versus orthotopic neobladders within each cohort. Estimated blood loss, total operative time, inpatient length of stay and pathologic T and N staging did not statistically differ between cohorts. Overall, there was no difference in the rates of postoperative ileus, ureteral stricture, anastomotic leak, infectious complications, and 30-day readmission rates between the groups.
Conclusions
Tubeless ureteroenteric anastomosis in patients undergoing robotic radical cystectomy with intracorporeal diversion doesn’t appear to increase the risk of anastomotic strictures or postoperative complications. Further prospective evaluation is warranted.
Convergent evolution of antibiotic resistance mechanisms between synthetic pyrrolobenzodiazepines (PBDs) and the naturally occurring albicidin in multidrug resistant Klebsiella pneumoniae
Authors: Surani Yasmin Miriam , Wand Matthew , Picconi Pietro , Palma Michele Di , Chiozzi Riccardo Zenezini , Hasan Md Mahbub , Maynard-Smith Michael , Steiner Roberto , Rahman Khondaker Miraz , Hind Charlotte , Sutton Mark 
Published: September 2024

Abstract

Klebsiella pneumoniae is designated as one of six priority ESKAPE pathogens by the World Health Organisation (WHO). It is the causative agent of a number of serious infections, including pneumonia, and worryingly strains are known to have resistance to the four major antibiotic classes. Pyrrolobenzodiazepines (PBDs) with a C8-linked aliphatic heterocycle have been developed as a new class of potent antibacterial compounds. They are active against multidrug resistant (MDR) Gram-negative pathogens, including K. pneumoniae. When K. pneumoniae isolates were exposed to PBDs, they acquired resistance, with an increase in minimum inhibitory concentration (MIC) from 1-4 µg/mL to >32 µg/mL. Resistant strains showed mutations in genes associated with resistance to the phytotoxin albicidin, specifically tsxand merR-family regulator albA. Heterologous expression of AlbA in Escherichia coli, and introduction of a proposed resistance-mediating single-nucleotide polymorphism (SNP) (AlbA L120Q) into the genome of a sensitive K. pneumoniae strain confers both PBD and albicidin resistance. Given the parallels between these two structurally unrelated compound classes, these mechanisms may offer resistance to further antibiotics in K. pneumoniae and should be considered in future antibiotic discovery.
Transcriptome and DNA methylation profiling during the NSN to SN transition in mouse oocytes
Authors: Demond Hannah , Khan Soumen , Castillo-Fernandez Juan , Hanna Courtney W. , Kelsey Gavin 
Published: September 2024

Abstract

Background: During the latter stages of their development, mammalian oocytes under dramatic chromatin reconfiguration, transitioning from a non-surrounded nucleolus (NSN) to a surrounded nucleolus (SN) stage, and concomitant transcriptional silencing. Although the NSN-SN transition is known to be essential for developmental competence of the oocyte, less is known about the accompanying molecular changes. Here we examine the changes in the transcriptome and DNA methylation during the NSN to SN transition in mouse oocytes.Results: To study the transcriptome and DNA methylation dynamics during the NSN to SN transition, we used single-cell (sc)M&T-seq to generate scRNA-seq and sc-bisulphite-seq (scBS-seq) data from GV oocytes classified as NSN or SN by Hoechst staining of their nuclei. Transcriptome analysis showed a lower number of detected transcripts in SN compared with NSN oocytes as well as downregulation of 576 genes, which were enriched for processes related to mRNA processing. We used the transcriptome data to generate a classifier that can infer chromatin stage in scRNA-seq datasets. The classifier was successfully tested in multiple published datasets of mouse models with a known skew in NSN:SN ratios. Analysis of the scBS-seq data showed increased DNA methylation in SN compared to NSN oocytes, which was most pronounced in regions with intermediate levels of methylation. Overlap with chromatin immunoprecipitation and sequencing (ChIP-seq) data for the histone modifications H3K36me3, H3K4me3 and H3K27me3 showed that regions gaining methylation in SN oocytes are enriched for overlapping H3K36me3 and H3K27me3, which is an unusual combination, as these marks do not typically coincide.Conclusions: We characterise the transcriptome and DNA methylation changes accompanying the NSN-SN transition in mouse oocytes. We develop a classifier that can be used to infer chromatin status in single-cell or bulk RNA-seq data, enabling identification of altered chromatin transition in genetic knock-outs, and a quality control to identify skewed NSN-SN proportions that could otherwise confound differential gene expression analysis. We identify late-methylating regions in SN oocytes that are associated with an unusual combination of chromatin modifications, which may be regions with high chromatin plasticity and transitioning between H3K27me3 and H3K36me3, or reflect heterogeneity on a single-cell level.
Parallel trusted node approach for satellite quantum key distribution
Authors: De Santis Gianluca , Kravtsov Konstantin , Pyka Sana Amairi , Grieve James A. 
Published: September 2024

Abstract

Quantum key distribution (QKD) via satellite links is the only currently viable solution to create quantumbacked secure communication at a global scale. To achieve intercontinental coverage with available technology one must adopt a “flying trusted node” paradigm, in which users fully trust the satellite platform. Here, inspired by the concept of distributed secret sharing and the imminent projected launch of several QKD-equipped satellites, we propose a parallel trusted node approach, in which key distribution is mediated by several satellites in parallel. This has the effect of distributing the trust, removing single points of failure and reducing the necessary assumptions. In addition, we discuss the versatility that an optical ground station should provide to execute such a protocol and, in general, to be fully integrated into a multi-party global quantum network.
Metabolic Syndrome and Its Component Factors Among Corporate Company Employees in Kampala Uganda
Authors: Jemimah Kiboss Kyeyune , Zandile June-Rose Mchiza , Bashir Mwambi , Prossy Merab Ingabire , Florence Wamuyu Githinji , Nakaggwa Florence , Ivan Gabriel Busulwa , Rose Clarke Nanyonga 
Published: September 2024

Abstract

Background Metabolic syndrome (MetS), a cluster of metabolic dysregulations indicative of increased cardiometabolic risk is on the rise in Sub-Saharan Africa. The study aimed to determine the prevalence of MetS and its components, among corporate employees in Kampala, Uganda.Methods A cross-sectional survey was undertaken among 408 adults who were employees from seven corporate companies in Kampala, using the WHO STEPwise NCD screening approach. Metabolic syndrome was measured using the National Cholesterol Education Program Adult Treatment Panel (ATPIII) and the International Diabetes Federation (IDF) criteria with the waist circumference (WC) cut-off points adapted for Sub-Saharan African populations.Results The mean population age (standard deviation [SD]) of the respondents was 34years (± 8.87) years and 52% of them were females. The prevalence of MetS was 22.8% (NCEP ATPIII) and 28.4% (IDF). Of the respondents who did not have MetS, 75% had at least one metabolic dysregulation. Of those respondents with MetS, only 31% perceived that they were at risk of this cluster of metabolic dysregulations. In this analysis, we observed that the systolic blood pressure (BP) and the body mass index (BMI) correlated strongly (r = 0.81 and r = 0.71) with the diastolic BP and waist circumference (WC), respectively. Age on the other hand correlated fairly with the WC and BMI (both r values = 0.46). Principal component analysis showed that the greatest loadings in principle factors one, two and three were from central obesity, with low HDL-C explaining 60.8% variance in the population. Age, BMI, family history of having cardiometabolic disorders, and perceived cardiometabolic disease risk (CMR) were associated with an increased risk of MetS by 5, 8.86, 1.55, and 2.73 (all P values were < 0.05) respectively in this group of respondents. These risks remained for age, BMI and perceived CMR after removing the confounding effects of education status, marital status and family history of cardiometabolic disease. Being single on the other hand, was associated with a reduced risk of MetS (0.23, p < 0.009).Conclusion While the primary contributors to the high prevalence of MetS among corporate employees in Kampala, Uganda were the high BP, high WC and high fasting blood sugar (FBS); age, BMI and perceived CMR were the key determinants of MetS. Future MetS interventions should aim to control and monitor obesity indicators in this population. Additionally, the findings inform targeted screening parameters for cardiometabolic risk assessment and suggest the need for further research into a weighted algorithm for MetS in this population.
Conceptual Design of Wood Pillar Foundation inSwampland: A Construction Solution for Drainage Improvement with Adaptation of Rain Garden’s Concept
Authors: Nursyarif Agusniansyah. , Syarief Akhmad , Noor Rijali , Annisa Nova 
Published: September 2024

Abstract

Building on swampy land is a major challenge due to the soft and waterlogged soil properties. Traditional approaches using wooden pile foundations have long been used but new technology with a more environmentally friendly and efficient approach needs to be applied to swampy land. The objectives of this research are: to obtain a conceptual model of modified wooden pile foundation construction combined with concrete, to identify the impact of soil condition changes due to foundation implementation and the swampy groundwater environment, and to determine the application of the 'constructed wetland' and rain garden adaptation methods to the foundation model concept. In this research, a qualitative method was used with a descriptive approach based on literature studies and data collection through surveys/field observations. There is interdisciplinary collaboration from architectural, material, and environmental fields. The method conducted includes experimental testing of the foundation model concept. The research stages are: identifying variations in foundation design on wetland, survey/field study observation of foundations in Banjarmasin, architectural-material-environmental analysis of the foundation model concept with rain garden adaptation, and finally, conclusions and suggestions. The rain garden concept is an innovation that can help address waterlogging issues by enhancing the improvement of foundation construction and drainage in swamplands. In this paper, the use of galam wood, wooden pile foundations, and construction technology innovations to improve swampland conditions are discussed in detail.
Stock management practices and the availability of HIV viral load reagents and consumables in Burundi public laboratories, a cross-sectional study design, from January to December 2023
Authors: Niyonkuru Donatien , Mitiku Shiferaw , Ngenzi Joseph Lune , Niragire Francois , Mukamurigo Judith 
Published: September 2024

Abstract

Background
Regularly monitoring viral load is crucial for managing HIV treatment and preventing drug resistance. Burundi National AIDS Control Program reports a gap in achieving viral load testing coverage for people living with HIV/AIDS (PLWHA).
Objective
This study assessed the stock management practices and the availability of HIV testing reagents and consumables in Burundi public health laboratories with conventional equipment.
Methods
The study involved four public laboratories performing HIV viral load tests. Primary data were collected electronically using surveys, and secondary data were collected from stock management tools.
Results
The study identified significant deficiencies in stock management practices. For example, 38% of facilities must update stock cards, and 75% lack daily consumption sheets. Furthermore, practices for organizing, storing, and setting stock levels for reagents and consumables needed to be improved.
Conclusion
These findings suggest that poor stock management practices likely contribute to the limited availability of HIV viral load testing in Burundi. Improving these practices can ensure a consistent supply of testing materials, ultimately leading to better monitoring of PLHIV health and potentially improved treatment outcomes.
Prevalence and antibiotic resistance profile of Salmonella spp. strains isolated from the vegetable food chain in Niamey, Niger
Authors: Abdoulaye Alio Almou , Sanda Abdel-Kader Alio , Chaibou Yaou , Rabiou Maman Moustapha , Fody Alio Mahamadou , Seybou Djalifa Hamidou , Seini Haoua Sabo , Sadou Hassimi 
Published: September 2024

Abstract

Background
Salmonellosis is the main cause of collective food poisoning in humans. They are bacterial infections caused by various Salmonella species. The overall aim of this study was to determine the prevalence and antibiotic resistance phenotype of Salmonella strains isolated from vegetables in the urban community of Niamey.
Methods
To this end, one hundred and twenty-four (124) samples of some vegetables (carrot, lettuce, onion and tomato) were taken from market garden sites and markets in Niamey. Salmonella were tested using the ISO 6579:2017 4-step method: pre-enrichment, enrichment, isolation and biochemical identification, and the antibiotic resistance phenotype was determined using the standard Kirby-Bauer agar diffusion method.
Results
Some sampling sites showed high Salmonella prevalences, notably site 1 (75.00%) and market 4 (40.00%). The overall level of Salmonella contamination in vegetables was 13.71%, including carrot (25.00%) and lettuce (15.38%) samples. The most common resistance phenotypes were Ceftriazone (100%), Amoxicillin + Clavulanic Acid (76.92%), Ciprofloxacin (46.15%), Aztreonam (33.33%) and Imipenem (30.77%).
Conclusion
The presence of multidrug-resistant Salmonella strains in vegetables reflects the potential risk associated with their consumption. It is important to research the potential resistance and virulence genes of these strains for better management of infectious diseases.
A First Approximation of Genetic Diversity and Population Structure to Identify Management Units for Western North Carolina Timber Rattlesnakes (Crotalus horridus)
Authors: Koch Hudson , O’Neill Alex , Estep Matt C. 
Published: September 2024

Abstract

The Timber Rattlesnake (Crotalus horridus) is the only rattlesnake species inhabiting the temperate deciduous forests of Eastern North America and faces conservation challenges across its range. In North Carolina, the Timber Rattlesnake is listed as a Species of Special Concern, with habitat loss and fragmentation from human encroachment its primary threats. To effectively manage and conserve Timber Rattlesnake populations, land managers must first understand their genetic diversity and population structure. This study utilized 19 microsatellite markers to estimate genetic diversity parameters from 125 individuals sampled opportunistically across a broad geographic area in Western North Carolina. Our findings revealed robust genetic diversity and identified three management units, which will assist land managers in formulating effective conservation action plans.
Comparative assessment of antibacterial drugs used at the hos-pital level before and during COVID-19, according to the WHO AWaRe classification
Authors: Balapasheva Aigerim , Ziganshina Liliya , Mussina Aigul , Smagulova Gaziza , Kulnazarova Mariya , Balymbetova Lazzat 
Published: September 2024

Abstract

Background
This study presents a comprehensive analysis of antibiotic consumption in a dispensary hospital in Aktobe, Kazakhstan, from 2019 to 2021, utilizing the World Health Organization's (WHO) "Access, Watch, and Reserve" (AWaRe) classification.
Objective
The primary objective of this study was to assess the patterns of antibiotic use in the hospital and to determine the extent to which the AWaRe classification could be effectively implemented in Kazakhstan to guide optimal antibiotic use and establish performance targets.
Methods
The study analyzed data on the consumption of systemic antibacterial drugs in the dispensary hospital from 2019 to 2021. The usage of antibiotics was classified according to the WHO's AWaRe classification, which categorizes antibiotics into three groups: "Access," "Watch," and "Reserve."
Results
The study revealed a concerning trend in the irrational consumption of systemic antibiotics. There was a notable decrease in the use of "Access" antibiotics from 39.13% in 2019 to 22% in 2021, which is contrary to WHO recommendations. Conversely, the use of "Watch" antibiotics increased, particularly during the COVID-19 pandemic. The most commonly used antibiotics were cephalosporins, fluoroquinolones, and carbapenems.
Conclusion
The findings underscore the urgent need for implementing the AWaRe classification database in Kazakhstan to guide optimal antibiotic use, set performance targets, and establish a working group to monitor and control the prescription and use of antibacterial drugs. This study contributes to the global understanding of antibiotic consumption patterns and the challenges faced in adhering to international guidelines, particularly in the context of a pandemic.