bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copeptin, insulin-like growth factor binding protein-1 and sitagliptin: A report from the BEta-cell function in Glucose abnormalities and Acute Myocardial Infarction study
Authors: Arnetz Lisa , Hage Camilla , Brismar Kerstin , Catrina Sergiu-Bogdan , Norhammar Anna , Lundman Pia , Wallander Märit , Ryden Lars , Mellbin Linda 
Published: April 2016

Purpose: To investigate whether sitagliptin affects copeptin and osmolality, suggesting arginine vasopressin activation and a potential for fluid retention, compared with placebo, in patients with a recent acute coronary syndrome and newly discovered type 2 diabetes or impaired glucose tolerance. A second aim was to confirm whether copeptin correlated with insulin-like growth factor binding protein-1. Methods: Fasting blood samples were used from the BEta-cell function in Glucose abnormalities and Acute Myocardial Infarction trial, in which patients recently hospitalized due to acute coronary syndrome and with newly detected abnormal glucose tolerance were randomized to sitagliptin 100 mg once daily ( n = 34) or placebo ( n = 37). Copeptin, osmolality and insulin-like growth factor binding protein-1 were analysed at baseline and after 12 weeks. Results: Copeptin and osmolality were unaffected by sitagliptin. There was no correlation between copeptin and insulin-like growth factor binding protein-1. Conclusion: Sitagliptin therapy does not appear to be related to activation of the arginine vasopressin system.
Accelerated rehabilitation after arthroscopic Bankart repair in professional footballers
Authors: Gibson Jo , Kerss Jim , Morgan Chris , Brownson Peter 
Published: April 2016

Background Advances in arthroscopic surgery have resulted in biomechanically stronger repairs that might allow for accelerated rehabilitation protocols and hence faster return to play. Evidence for such regimes in the shoulder, particularly in elite athletes, is lacking. Methods This prospective single surgeon (PB) series included 34 professional footballers undergoing an accelerated rehabilitation programme following arthroscopic soft tissue stabilization subsequent to traumatic anterior shoulder dislocation. Data were collected on time to regain elevation range, external rotation range, return to play and rate of recurrence. Results Mean follow-up time was 4.8 years (range 2 years to 10 years). Full range of forward elevation was regained at a mean of 5 weeks (range 3 weeks to 7 weeks) and external rotation range (in neutral) at a mean of 6 weeks (range 4 weeks to 8 weeks). Mean return to play time was 11 weeks (range 9 weeks to 14 weeks). Three players (9%) reported a recurrent episode of dislocation at a mean of 19 months. Conclusions An accelerated rehabilitation programme resulted in a return to play time of 11 weeks compared to previously reported times of between 5 months and 9 months in the contact sports population. A recurrence rate of 9% compares favourably to other published studies following similar surgery (5.1% to 28.6%) but which employed more conservative postoperative rehabilitation regimes.
A systematic review of image segmentation methodology, used in the additive manufacture of patient-specific 3D printed models of the cardiovascular system
Authors: Byrne N , Velasco Forte M , Tandon A , Valverde I , Hussain T 
Published: April 2016

Background Shortcomings in existing methods of image segmentation preclude the widespread adoption of patient-specific 3D printing as a routine decision-making tool in the care of those with congenital heart disease. We sought to determine the range of cardiovascular segmentation methods and how long each of these methods takes. Methods A systematic review of literature was undertaken. Medical imaging modality, segmentation methods, segmentation time, segmentation descriptive quality (SDQ) and segmentation software were recorded. Results Totally 136 studies met the inclusion criteria (1 clinical trial; 80 journal articles; 55 conference, technical and case reports). The most frequently used image segmentation methods were brightness thresholding, region growing and manual editing, as supported by the most popular piece of proprietary software: Mimics (Materialise NV, Leuven, Belgium, 1992–2015). The use of bespoke software developed by individual authors was not uncommon. SDQ indicated that reporting of image segmentation methods was generally poor with only one in three accounts providing sufficient detail for their procedure to be reproduced. Conclusions and implication of key findings Predominantly anecdotal and case reporting precluded rigorous assessment of risk of bias and strength of evidence. This review finds a reliance on manual and semi-automated segmentation methods which demand a high level of expertise and a significant time commitment on the part of the operator. In light of the findings, we have made recommendations regarding reporting of 3D printing studies. We anticipate that these findings will encourage the development of advanced image segmentation methods.
Update on the safety and efficacy of retroviral gene therapy for immunodeficiency due to adenosine deaminase deficiency
Authors: Cicalese Maria Pia , Ferrua Francesca , Castagnaro Laura , Pajno Roberta , Barzaghi Federica , Giannelli Stefania , Dionisio Francesca , Brigida Immacolata , Bonopane Marco , Casiraghi Miriam , Tabucchi Antonella , Carlucci Filippo , Grunebaum Eyal , Adeli Mehdi , Bredius Robbert G. , Puck Jennifer M. , Stepensky Polina , Tezcan Ilhan , Rolfe Katie , De Boever Erika , Reinhardt Rickey R. , Appleby Jonathan , Ciceri Fabio , Roncarolo Maria Grazia , Aiuti Alessandro 
Published: April 2016

Key PointsSurvival was 100% for 18 patients with ADA-SCID treated with genetically modified CD34+ cells (2.3-13.4 years follow up; median, 6.9 years). Long-term engraftment, immune reconstitution, and fewer severe infections were observed in 15 out of 18 patients without leukemic transformation.
G-DOC Plus – an integrative bioinformatics platform for precision medicine
Authors: Bhuvaneshwar Krithika , Belouali Anas , Singh Varun , Johnson Robert M. , Song Lei , Alaoui Adil , Harris Michael A. , Clarke Robert , Weiner Louis M. , Gusev Yuriy , Madhavan Subha 
Published: April 2016

Abstract
Background
G-DOC Plus is a data integration and bioinformatics platform that uses cloud computing and other advanced computational tools to handle a variety of biomedical BIG DATA including gene expression arrays, NGS and medical images so that they can be analyzed in the full context of other omics and clinical information.

Results
G-DOC Plus currently holds data from over 10,000 patients selected from private and public resources including Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA) and the recently added datasets from REpository for Molecular BRAin Neoplasia DaTa (REMBRANDT), caArray studies of lung and colon cancer, ImmPort and the 1000 genomes data sets. The system allows researchers to explore clinical-omic data one sample at a time, as a cohort of samples; or at the level of population, providing the user with a comprehensive view of the data.
G-DOC Plus tools have been leveraged in cancer and non-cancer studies for hypothesis generation and validation; biomarker discovery and multi-omics analysis, to explore somatic mutations and cancer MRI images; as well as for training and graduate education in bioinformatics, data and computational sciences. Several of these use cases are described in this paper to demonstrate its multifaceted usability.

Conclusion
G-DOC Plus can be used to support a variety of user groups in multiple domains to enable hypothesis generation for precision medicine research. The long-term vision of G-DOC Plus is to extend this translational bioinformatics platform to stay current with emerging omics technologies and analysis methods to continue supporting novel hypothesis generation, analysis and validation for integrative biomedical research. By integrating several aspects of the disease and exposing various data elements, such as outpatient lab workup, pathology, radiology, current treatments, molecular signatures and expected outcomes over a web interface, G-DOC Plus will continue to strengthen precision medicine research. G-DOC Plus is available at: https://gdoc.georgetown.edu.
The role of long-term label-retaining cells in the regeneration of adult mouse kidney after ischemia/reperfusion injury
Authors: Liu Xiangchun , Liu Haiying , Sun Lina , Chen Zhixin , Nie Huibin , Sun Aili , Liu Gang , Guan Guangju 
Published: April 2016

Abstract
Background
Label-retaining cells (LRCs) have been recognized as rare stem and progenitor-like cells, but their complex biological features in renal repair at the cellular level have never been reported. This study was conducted to evaluate whether LRCs in kidney are indeed renal stem/progenitor cells and to delineate their potential role in kidney regeneration.

Methods
We utilized a long-term pulse chase of 5-bromo-2'-deoxyuridine (BrdU)-labeled cells in C57BL/6J mice to identify renal LRCs. We tracked the precise morphological characteristics and locations of BrdU+LRCs by both immunohistochemistry and immunofluorescence. To examine whether these BrdU+LRCs contribute to the repair of acute kidney injury, we analyzed biological characteristics of BrdU+LRCs in mice after ischemia/reperfusion (I/R) injury.

Results
The findings revealed that the nuclei of BrdU+ LRCs exhibited different morphological characteristics in normal adult kidneys, including nuclei in pairs or scattered, fragmented or intact, strongly or weakly positive. Only 24.3 ± 1.5 % of BrdU+ LRCs co-expressed with Ki67 and 9.1 ± 1.4 % of BrdU+ LRCs were positive for TUNEL following renal I/R injury. Interestingly, we found that newly regenerated cells formed a niche-like structure and LRCs in pairs tended to locate in this structure, but the number of those LRCs was very low. We found a few scattered LRCs co-expressed Lotus tetragonolobus agglutinin (LTA) in the early phase of injury, suggesting differentiation of those LRCs in mouse kidney.

Conclusions
Our findings suggest that LRCs are not a simple type of slow-cycling cells in adult kidneys, indicating a limited role of these cells in the regeneration of I/R injured kidney. Thus, LRCs cannot reliably be considered stem/progenitor cells in the regeneration of adult mouse kidney. When researchers use this technique to study the cellular basis of renal repair, these complex features of renal LRCs and the purity of real stem cells among renal LRCs should be considered.
Relevance of HCN2-expressing human mesenchymal stem cells for the generation of biological pacemakers
Authors: Bruzauskaite Ieva , Bironaite Daiva , Bagdonas Edvardas , Skeberdis Vytenis Arvydas , Denkovskij Jaroslav , Tamulevicius Tomas , Uvarovas Valentinas , Bernotiene Eiva 
Published: April 2016

Abstract
Background
The transfection of human mesenchymal stem cells (hMSCs) with the hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) gene has been demonstrated to provide biological pacing in dogs with complete heart block. The mechanism appears to be the generation of the ion current (If) by the HCN2-expressing hMSCs. However, it is not clear how the transfection process and/or the HCN2 gene affect the growth functions of the hMSCs. Therefore, we investigated survival, proliferation, cell cycle, and growth on a Kapton® scaffold of HCN2-expressing hMSCs.

Methods
hMSCs were isolated from the bone marrow of healthy volunteers applying a selective cell adhesion procedure and were identified by their expression of specific surface markers. Cells from passages 2–3 were transfected by electroporation using commercial transfection kits and a pIRES2-EGFP vector carrying the pacemaker gene, mouse HCN2 (mHCN2). Transfection efficiency was confirmed by enhanced green fluorescent protein (EGFP) fluorescence, quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). After hMSCs were transfected, their viability, proliferation, If generation, apoptosis, cell cycle, and expression of transcription factors were measured and compared with non-transfected cells and cells transfected with pIRES2-EGFP vector alone.

Results
Intracellular mHCN2 expression after transfection increased from 22.14 to 62.66 ng/mg protein (p < 0.05). Transfection efficiency was 45 ± 5 %. The viability of mHCN2-transfected cells was 82 ± 5 %; they grew stably for more than 3 weeks and induced If current. mHCN2-transfected cells had low mitotic activity (10.4 ± 1.24 % in G2/M and 83.6 ± 2.5 % in G1 phases) as compared with non-transfected cells (52–53 % in G2/M and 31–35 % in G1 phases). Transfected cells showed increased activation of nine cell cycle-regulating transcription factors: the most prominent upregulation was of AMP-dependent transcription factor ATF3 (7.11-fold, p = 0.00056) which regulates the G1 phase. mHCN2-expressing hMSCs were attached and made anchorage-dependent connection with other cells without transmigration through a 12.7-μm thick Kapton® HN film with micromachined 1–3 μm diameter pores.

Conclusions
mHCN2-expressing hMSCs preserved the major cell functions required for the generation of biological pacemakers: high viability, functional activity, but low proliferation rate through the arrest of cell cycle in the G1 phase. mHCN2-expressing hMSCs attached and grew on a Kapton® scaffold without transmigration, confirming the relevance of these cells for the generation of biological pacemakers.
Othello syndrome after STN DBS – psychiatric side-effects of DBS and methods of dealing with them
Authors: Antosik-Wójcińska Anna , Święcicki Łukasz , Bieńkowski Przemysław , Mandat Tomasz , Sołtan Emilia 
Published: April 2016

CelGłęboka stymulacja mózgu jest metodą terapeutyczną stosowaną od dziesięcioleci w chorobach neurologicznych, takich jak choroba Parkinsona czy dystonia. Pomimo wielu publikacji dotyczących DBS, wciąż niewiele jest publikacji dotyczących objawów psychotycznych jako powikłania stymulacji, wciąż brak jest także standardów postępowania w przypadku ich wystąpienia.MetodaW artykule prezentujemy przypadek pacjenta z chorobą Parkinsona, u którego po rozpoczęciu stymulacji pojawiły się objawy psychotyczne przypominające zespół Otella oraz przedstawiamy podjętą strategię terapeutyczną (kontynuowanie stymulacji, dołączenie leczenia kwetiapiną).WynikiW omówionym przypadku obserwowano ustąpienie objawów psychotycznych,bez pogorszenia w zakresie objawów neurologicznych. Objawy psychotyczne nie nawróciły w ciągu 1 roku obserwacji.WnioskiZ uwagi na fakt, że obecnie prowadzone są zaawansowane prace nad wykorzystaniem DBS w psychiatrii, zwłaszcza w grupie pacjentów z depresją oporną na leczenie, konieczne jest opracowanie jasnej strategii postępowania w przypadku pojawienia się działań niepożądanych po rozpoczęciu stymulacji. Obecnie większość autorów opowiada się za czasowym lub stałym wyłączeniem stymulatora. W opinii autorów w niektórych przypadkach możliwe jest skuteczne leczenie objawów psychotycznych bez konieczności rezygnacji z korzyści związanych z dalszym prowadzeniem stymulacji. Do tej pory postępowanie takie było jednak podejmowane i opisywane na tyle rzadko, że trudno na podstawie pojedynczych przypadków formułować wnioski, które można odnieść do całej populacji pacjentów poddawanych DBS. Dopiero systematyczne badania uwzględniające ocenę objawów psychotycznych z wykorzystaniem skal oraz analizujące otrzymywane leczenie oraz parametry stymulacji mogłyby dać pogląd jaka strategia postępowania byłaby najwłaściwsza w przypadku wystąpienia psychozy w następstwie DBS.
Psychiatric disturbances as a first clinical symptom of Wilson’s disease – case report.
Authors: Litwin Tomasz , Dzieżyc Karolina , Karliński Michał , Szafrański Tomasz , Członkowska Anna 
Published: April 2016

CelWstęp: Choroba Wilsona (chW) jest dziedzicznym zaburzeniem metabolizmu miedzi z szerokim spektrum objawów klinicznych, przede wszystkim wątrobowych i neurologicznych. Zaburzenia psychiatryczne chW występują rzadziej i nie są patognomoniczne. Tym niemniej, w prawie 20% przypadków mogą być pierwszymi objawami chW. Celem niniejszej pracy jest zwrócenie uwagi na konieczność włączenia chW do diagnostyki różnicowej objawów psychiatrycznych u młodych pacjentów oraz zachowania daleko posuniętej ostrożności przy włączaniu leków psychiatrycznych w chW.MetodaMetody: Opis przypadku pacjenta z chW o pierwotnej manifestacji psychiatrycznej.WynikiWyniki: W pracy przedstawiono opis przypadku 26-letniego pacjenta, u którego po 3 latach od rozpoczęcia farmakoterapii zespołu depresyjnego wystąpiły objawy pozapiramidowe prowadzące do rozpoznania chW. W ciągu kolejnych miesięcy wystąpił epizod maniakalny, którego początkowe leczenie atypowymi neuroleptykami i anksjolitykami przyniosło dobry efekt psychiatryczny, jednak równolegle doszło do ciężkiego zaostrzenia objawów neurologicznych. Długoterminowe leczenie kwasem walproinowym i olanzapiną, w połączeniu z rehabilitacją doprowadziło do wyrównania stanu psychicznego oraz znacznej poprawy stanu neurologicznego.WnioskiWnioski: ChW powinna być zawsze brana pod uwagę w diagnostyce różnicowej zaburzeń psychiatrycznych, szczególnie u młodych pacjentów ze współistniejącymi objawami pozapiramidowymi lub/i wątrobowymi. W przypadku konieczności leczenia psychiatrycznego pacjentów z rozpoznaną chW należy zachować szczególną ostrożność podczas włączania leków o dużym powinowactwie do receptorów dopaminergicznych D2 ze względu na realne ryzyko wywołania ciężkich, często nieodwracalnych powikłań neurologicznych.
Behavioral variant frontotemporal dementia with dominant gait disturbances - case report.
Authors: Guenter Wojciech , Betscher Ewa , Bochniak Paweł , Bonek Robert 
Published: April 2016

CelWariant behawioralny otępienia czołowo-skroniowego to heterogenne pod względem klinicznym i neuropatologicznym schorzenie neurozwyrodnieniowe charakteryzujące się postępującymi zaburzeniami zachowania i funkcji wykonawczych. Stanowi drugą co do częstości, po otępieniu typu alzheimerowskiego, przyczynę demencji o podłożu neurozwyrodnieniowym. Nietypowy przebieg choroby, w tym przypadki pacjentów z innymi objawami nakładającymi się na obraz kliniczny, niesie za sobą wyzwanie w procesie diagnostycznym. Celem pracy jest przedstawienie przypadku pacjentki z wariantem behawioralnym otępienia czołowo-skroniowego, u której zaburzenia chodu stanowiły zasadniczą dolegliwość maskując zaburzenia zachowania i funkcji poznawczych.MetodaOpis przypadkuWynikiW poniższym artykule prezentujemy przypadek pacjentki z wariantem behawioralnym otępienia czołowo-skroniowego, która przedstawiała zaburzenia chodu jako zasadniczy objaw schorzenia. Ten nietypowy objaw maskował obecność zaburzeń zachowania oraz funkcji poznawczych. Zaburzenia chodu występują często w późnych stadiach zespołów otępiennych. Wynikają wówczas z apraksji chodu, zespołu pozapiramidowego lub uszkodzenia neuronu ruchowego. Nie stanowią jednak zasadniczego objawu w przebiegu wariantu behawioralnego otępienia czołowo-skroniowego z wyjątkiem terminalnego stadium.WnioskiPrzedstawiony przypadek podkreśla znaczenie dokładnie zebranego i odpowiednio ukierunkowanego wywiadu z pacjentem i jego rodziną. Wariant behawioralny otępienia czołowo-skroniowego powinien być uwzględniany w przypadkach zaburzeń chodu o niejasnej etiologii.
A Red Handkerchief made with Soviet threads: Kazantzakis’s (and Istrati’s) screenplay on the Greek Revolution of 1821
Authors: Mini Panayiota 
Published: April 2016

Abstract
This article undertakes a close examination of Nikos Kazantzakis’s first screenplay, Kokino Mandili/‘Red Handkerchief’ (1928), more than three-quarters of which is preserved in a typed manuscript at the Nikos Kazantzakis Museum Foundation in Iraklion, Crete. Kazantzakis wrote Kokino Mandili in French in Russia for the national film organization VUFKU, with the collaboration of Panait Istrati. The screenplay, which was never shot, is a Marxist narrative of the Greek Revolution of 1821. The examination of the story, plot and imagery of Kokino Mandili shows that Kazantzakis borrowed freely from major Soviet avant-garde films, primarily Eisenstein’s The Battleship Potemkin and October and Pudovkin’s Mother and The End of St. Petersburg. He altered facts and traditional beliefs about the 1821 revolution for ideological purposes, constructed scenes through a few characteristic details, and employed montage techniques to suggest rhythm and juxtapositions and to translate ideas into images. At the same time, Kazantzakis combined the borrowings from Soviet cinema with some idealistic conceptions, thus producing a mixed artwork that alluded to both Marxist and anti-rationalist theories. In addition to contributing to our appreciation of Kazantzakis’s debts to Soviet cinema and fusion of different traditions in expressing his world-view, Kokino Mandili helps us to identify some key sources of the imagery and rhythm of his subsequent, non-filmic works and reflect on the broader issue of cinema’s impact on his literary creations.
From Utopia to Uchronia: After-images of revolutionary history in contemporary Mexican film
Authors: Schaefer Claudia , Rodríguez-Hernández Raúl 
Published: April 2016

Abstract
Both still and moving images in recent Mexican films have been characterized by the displacement of the established post-Revolutionary cinematic utopian space to timeimages (uchronia). Cinematic techniques at the disposal of contemporary directors have contributed to the portrayal and experience of temporal expectation relocated in much darker, stormier and perplexing visual sequences, with elliptical looping taking the place of narrative chronology. In contrast to Golden Age Mexican cinema of the 1930s and 1940s, and to the muralist aesthetic of photographers and directors alike, the films of Carlos Reygadas and Rodrigo García embed inherited collective social dreams in inner worlds filled with battles, darkness and strife. Revolutionary utopian expectations for the sites of future political models are now made thick with temporal dislocation and a shift from movement-image to time-image.
The re-construal of ‘La Costurera’: A feminist re-interpretation of the role of the seamstress in El tiempo entre costuras/The Time Between the Seams (2013)
Authors: Kemp Laura Lee 
Published: April 2016

Abstract
Prominent within an emerging trend of ‘quality’ narrative fiction productions in Spanish television, the recent Antena 3 adaptation of the bestselling novel El tiempo entre costuras/The Time Between the Seams offers a stylish feminist reclamation of 1940s fashion and a re-inscription of the culturally derided figure of the seamstress. The series was critically acclaimed, publically celebrated and commercially lucrative, creating a popular mainstream reinterpretation of the role of women during the Civil War and early Francoism. This article will demonstrate how both compositional and narrative interpretations of Joan Riviere’s masquerade are intrinsic within the series, and also fundamental in allowing the production to build a rich viewing experience. If the motif of costume is considered within a performative space, it can be interpreted as a denial of the persistent androcentric reading of fashion and of those women who worked in the industry under Franco. This article argues, therefore, that the protagonist of El tiempo entre costuras has emerged as an unlikely feminist icon within popular narrative screen fiction.