bluuebunny/crossref_metadata_2025_split huggingface dataset:
KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats
Authors: Tian Na-Xi , Xu Yu , Yang Jin-Yu , Li Lu , Sun Xiao-Hong , Wang Yun , Zhang Ying 
Published: January 2018

Potassium voltage-gated channel interacting protein 3 (KChIP3), also termed downstream regulatory element antagonist modulator (DREAM) and calsenilin, is a multifunctional protein belonging to the neuronal calcium sensor (NCS) family. Recent studies revealed the expression of KChIP3 in dorsal root ganglion (DRG) neurons, suggesting the potential role of KChIP3 in peripheral sensory processing. Herein, we show that KChIP3 colocalizes with transient receptor potential ion channel V1 (TRPV1), a critical molecule involved in peripheral sensitization during inflammatory pain. Furthermore, the N-terminal 31-50 fragment of KChIP3 is capable of binding both the intracellular N and C termini of TRPV1, which substantially decreases the surface localization of TRPV1 and the subsequent Ca2+influx through the channel. Importantly, intrathecal administration of the transmembrane peptide transactivator of transcription (TAT)-31-50 remarkably reduces Ca2+influx via TRPV1 in DRG neurons and alleviates thermal hyperalgesia and gait alterations in a complete Freund's adjuvant-induced inflammatory pain model in male rats. Moreover, intraplantar injection of TAT-31-50 attenuated the capsaicin-evoked spontaneous pain behavior and thermal hyperalgesia, which further strengthened the regulatory role of TAT-31-50 on TRPV1 channel. In addition, TAT-31-50 could also alleviate inflammatory thermal hyperalgesia inkcnip3−/−rats generated in our study, suggesting that the analgesic effect mediated by TAT-31-50 is independent of endogenous KChIP3. Our study reveals a novel peripheral mechanism for the analgesic function of KChIP3 and provides a potential analgesic agent, TAT-31-50, for the treatment of inflammatory pain.SIGNIFICANCE STATEMENTInflammatory pain arising from inflamed or injured tissues significantly compromises the quality of life in patients. This study aims to elucidate the role of peripheral potassium channel interacting protein 3 (KChIP3) in inflammatory pain. Direct interaction of the KChIP3 N-terminal 31-50 fragment with transient receptor potential ion channel V1 (TRPV1) was demonstrated. The KChIP3–TRPV1 interaction reduces the surface localization of TRPV1 and thus alleviates heat hyperalgesia and gait alterations induced by peripheral inflammation. Furthermore, the transmembrane transactivator of transcription (TAT)-31-50 peptide showed analgesic effects on inflammatory hyperalgesia independently of endogenous KChIP3. This work reveals a novel mechanism of peripheral KChIP3 in inflammatory hyperalgesia that is distinct from its classical role as a transcriptional repressor in pain modulation.
Discrete Modules and Mesoscale Functional Circuits for Thermal Nociception within Primate S1 Cortex
Authors: Yang Pai-Feng , Wu Ruiqi , Wu Tung-Lin , Shi Zhaoyue , Chen Li Min 
Published: January 2018

This study addresses one long-standing question of whether functional separations are preserved for somatosensory modalities of touch, heat, and cold nociception within primate primary somatosensory (S1) cortex. This information is critical for understanding how the nature of pain is represented in the primate brain. Using a combination of submillimeter-resolution fMRI and microelectrode local field potential (LFP) and spike recordings, we identified spatially segregated cortical zones for processing touch and nociceptive heat and cold stimuli in somatotopically appropriate areas 3a, 3b, 1, and 2 of S1 in male monkeys. The distances between zones were comparable (∼3.4 mm) across stimulus modalities (heat, cold, and tactile), indicating the existence of uniform, modality-specific modules. Stimulus-evoked LFP maps validated the fMRI maps in areas 3b and 1. Isolation of heat and cold nociceptive neurons from the fMRI zones confirmed the validity of using fMRI to probe nociceptive regions and circuits. Resting-state fMRI analysis revealed distinct intrinsic functional circuits among functionally related zones. We discovered distinct modular structures and networks for thermal nociception within S1 cortex, a finding that has significant implications for studying chronic pain syndromes and guiding the selection of neuromodulation targets for chronic pain management.SIGNIFICANCE STATEMENTPrimate S1 subregions contain discrete heat and cold nociceptive modules. Modules with the same properties exhibit strong functional connection. Nociceptive fMRI response coincides with LFP and spike activities of nociceptive neurons. Functional separation of heat and cold pain is retained within primate S1 cortex.
Recruitment of Additional Corticospinal Pathways in the Human Brain with State-Dependent Paired Associative Stimulation
Authors: Kraus Dominic , Naros Georgios , Guggenberger Robert , Leão Maria Teresa , Ziemann Ulf , Gharabaghi Alireza 
Published: January 2018

Standard brain stimulation protocols modify human motor cortex excitability by modulating the gain of the activated corticospinal pathways. However, the restoration of motor function following lesions of the corticospinal tract requires also the recruitment of additional neurons to increase the net corticospinal output. For this purpose, we investigated a novel protocol based on brain state-dependent paired associative stimulation.Motor imagery (MI)-related electroencephalography was recorded in healthy males and females for brain state-dependent control of both cortical and peripheral stimulation in a brain–machine interface environment. State-dependency was investigated with concurrent, delayed, and independent stimulation relative to the MI task. Specifically, sensorimotor event-related desynchronization (ERD) in the β-band (16–22 Hz) triggered peripheral stimulation through passive hand opening by a robotic orthosis and transcranial magnetic stimulation to the respective cortical motor representation, either synchronously or subsequently. These MI-related paradigms were compared with paired cortical and peripheral input applied independent of the brain state. Cortical stimulation resulted in a significant increase in corticospinal excitability only when applied brain state-dependently and synchronously to peripheral input. These gains were resistant to a depotentiation task, revealed a nonlinear evolution of plasticity, and were mediated via the recruitment of additional corticospinal neurons rather than via synchronization of neuronal firing. Recruitment of additional corticospinal pathways may be achieved when cortical and peripheral inputs are applied concurrently, and during β-ERD. These findings resemble a gating mechanism and are potentially important for developing closed-loop brain stimulation for the treatment of hand paralysis following lesions of the corticospinal tract.SIGNIFICANCE STATEMENTThe activity state of the motor system influences the excitability of corticospinal pathways to external input. State-dependent interventions harness this property to increase the connectivity between motor cortex and muscles. These stimulation protocols modulate the gain of the activated pathways, but not the overall corticospinal recruitment. In this study, a brain–machine interface paired peripheral stimulation through passive hand opening with transcranial magnetic stimulation to the respective cortical motor representation during volitional β-band desynchronization. Cortical stimulation resulted in the recruitment of additional corticospinal pathways, but only when applied brain state-dependently and synchronously to peripheral input. These effects resemble a gating mechanism and may be important for the restoration of motor function following lesions of the corticospinal tract.
O critério da fonte e o seu âmbito de aplicação
Authors: Azevedo Patrícia Anjos 
Published: January 2018

Resumo: Fruto da sua soberania, os diferentes Estados procuram estabelecer uma ligação efetiva com determinado facto económico para que possam exercer o seu poder tributário.Tal ligação pode verificar-se com base na ocorrência desse facto no território desse Estado (conexão objetiva), ou em função da pessoa com quem se relaciona o facto tributário (conexão subjetiva).Assim, é com base nos aspetos determinantes da soberania estadual que podemos identificar os elementos de conexão de natureza objetiva (referentes ao facto tributável – critério da fonte) e de natureza subjetiva (referentes ao titular do rendimento – critério da residência).Os tratados internacionais em matéria fiscal apresentam uma aplicação combinada da tributação com base na residência e na fonte. Neste âmbito, são conferidos alguns direitos ao país da fonte, sendo deixada para o país da residência a tarefa de evitar a dupla tributação. Resumen: Fruto de su soberanía, los diferentes Estados tratan de establecer una conexión efectiva con cierto hecho económico que puede ejercer su potestad tributaria.Tal conexión puede ocurrir en base a la ocurrencia de los mismos en dicho Estado (conexión objetiva), o porque la persona que se relaciona la operación gravada (conexión subjetiva).Por lo tanto, se basa en aspectos cruciales de la soberanía del Estado que podemos identificar los elementos de conexión de la naturaleza objetiva (por el hecho imponible – criterio de la fuente) y de carácter subjetivo (para los ingresos del titular –criterio de la residencia).Los tratados fiscales internacionales tienen una aplicación combinada de los impuestos sobre la base de residencia y la fuente. En este contexto, y conferían ciertos derechos al país de la fuente, quedando al país de residencia de la tarea de evitar la doble imposición. Abstract: As a result of their sovereignty, the different States seek to establish an effective link with a certain economic fact so that they can exercise their tax power.Such a link may be based on the occurrence of that fact in the territory of that State (objective connection), or in relation to the person with whom the taxable event is related (subjective connection).Thus, it is on the basis of the determinant aspects of state sovereignty that we can identify the connection elements of an objective nature (referring to the taxable factor – source criterion) and subjective nature (referring to the income holder – residence criterion).International tax treaties have a combined application of taxation based on residence and source. In this context, some rights are conferred to the country of origin, but the task of avoiding double taxation is left to the country of residence. Palavras-chave: soberania, fatores de conexão, critério da fonte, convenções para evitar a dupla tributação. Palabras clave: soberanía, puntos de conexión, criterio de la fuente, convenios para evitar la doble imposición. Key-words: sovereignty, connection factors, source criterion, conventions to avoid double taxation.
Ecosistema de los Comunes y Democracia bajo la perspectiva del enfoque de capacidad de Amartya Sen / Common Ecosystem and Democracy under the perspective of Amartya Sen Capacity Approach
Authors: Carvajal Muñoz María Rosario 
Published: January 2018

El texto abre un debate en torno a la política y lo común, focalizándose en las repercusiones que el uso de lo común tiene para las libertades de los individuos. Se hace especial hincapié en el uso común, público o privado de las nuevas tecnologías. En este sentido, un punto de arranque importante en el rearme de lo común fue el movimiento social del 15M en 2011, que reivindicó otro modelo de concebir la política. A partir de ahí se ha dado un incremento notorio de propuestas de lo común. Su propósito principal ha sido, y es, crear espacios compartidos en comunidad, donde debatir sobre cuestiones sociales de interés común, y abriendo espacios de libertad a una sociedad heterogénea. Las nuevas tecnologías tienen un papel destacado en estos nuevos formatos de la política. No obstante, el uso de Internet, y de los medios de comunicación en general, no están exentos de peligros, porque en ellos, como se constatan en otras instancias, están reflejadas las relaciones de poder, con un importante peso de los intereses comerciales. Esta realidad limita las libertades de los individuos y hacen más necesarios los espacios comunes de resistencia ciudadana. En este particular, el enfoque de capacidad de Amartya Sen, y el planteamiento teórico en el que se inscribe, constituyen herramientas útiles para reflexionar sobre la necesaria presencia de espacios de lo común en nuestras vidas, con objeto de preservar libertades básicas. Lo común amplía libertades, siguiendo las facetas de libertad de Sen, es por esto que se hace una defensa de los espacios comunes, también porque nos lleva a una democracia más participativa.
Is the expression of matrix metalloproteinases (MMP-2, -9) and tissue inhibitors of metalloproteinases (TIMP-1, -2, and -3) associated with angiogenesis and clinicopathological features for breast cancer?
Authors: Kuskunović-Vlahovljak Suada , Čamdžić Nina , Radović Svjetlana , Dorić Mirsad , Babić Mirsad , Lazović Salčin Edina , Džananović Lejla 
Published: January 2018

Introduction: Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are involved in the progression of several tumors, including breast cancer. Our aim was to investigate the association of immunohistochemical expression of protein MMP-2, and -9 and tissue inhibitors TIMP-1,-2,-3 by tumoral cells in the process of angiogenesis and to define their relation with clinicopathological features for breast cancer.
Methods: Immunohistochemical analysis of MMP-2,-9, TIMP-1,-2,-3, endoglin/CD105, estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) status was performed on 79 tissue samples of breast cancer with axillary lymph node dissection.
Results: Statistically significant difference was found between mean age of patients and tissue inhibitors of metalloproteinase (TIMP-1) expression status (p=0.008), i.e., women with TIMP-1 negative tumors were on average younger (mean age 46.5) compared to women with TIMP-1 positive tumors (mean age 58.1); TIMP-2 expression status showed association with ER status (p=0.017), while TIMP-3 negative tumors were on average more frequently ER and PR negative (p=0.016; p=0.027). Status of protein expression of MMP-9 was associated with TIMP-1 protein expression status (p=0.033), i.e., breast cancers with overexpression of protein MMP-9 were more frequently TIMP-1 protein positive.
Conclusion: Only TIMPs were associated with clinicopathological features for breast cancer. TIMP-2 expression was associated with worse (TIMP-2 positive tumors were frequently ER-negative), while TIMP-3 expression in tumoral cells was associated with better clinicopathological features for breast cancer (TIMP-3 positive tumors were frequently ER and PR positive).
AVALIAÇÃO NUTRICIONAL DE IDOSOS RESIDENTES EM INSTITUIÇÕES DE LONGA PERMANÊNCIA
Authors: Lima Ana Priscila Marques , Gomes Karen Virginia Lopes , Pereira Francisco Gilberto Fernandes , Barros Livia Moreira , Silva Máguida Gomes da , Frota Natasha Marques 
Published: January 2018

<p>Objetivo: avaliar o estado nutricional de idosos residentes em nstituições de Longa Permanência. Método: estudo descritivo e transversal com abordagem quantitativa realizado de março a abril de 2016, com amostra de 78 idosos institucionalizados. A coleta de dados ocorreu com a aplicação do instrumento Mini Avaliação Nutricional durante visitas previamente agendadas. Resultados: predominou o sexo feminino, 44 (56%), e identificou-se que há risco de desnutrição, com média de avaliação global de 17,7 (DP ± 5,2). Houve associação estatisticamente<br />significativa entre o risco de desnutrição e as variáveis: sexo, idade e tempo de institucionalização, com p &lt; 0,05.<br />Conclusão: a institucionalização consiste em um fator fortemente associado a alterações no estado nutricional do idoso, o que torna necessária a adoção de medidas adaptativas pelas instituições de longa permanência para essa população no tocante ao suporte nutricional adequado que é importante para a manutenção do estado de saúde e da qualidade de vida.</p><p><br />Descritores: Avaliação nutricional. Nutrição do idoso. Institucionalização.</p>
NÍVEL DE DOR E GRAU DE INDEPENDÊNCIA FUNCIONAL DE PACIENTES SUBMETIDOS À CIRURGIA CARDÍACA
Authors: Chiapinotto Sabrina , Dallazen Fernanda , Teixeira Bodnar Emely , Winkelmann Eliane Roseli 
Published: January 2018

<p>Objetivo: avaliar a correlação da dor e independência funcional no período pré e pós-operatório dos pacientes submetidos à cirurgia cardíaca. Métodos: estudo prospectivo e não intervencionista composto por 24 pacientes que realizaram cirurgia cardíaca eletiva. Foram coletados os dados clínicos nos prontuários dos pacientes e realizada entrevista direta no pré-operatório e no pós-operatório. A intensidade de dor foi avaliada pela Escala Visual Numérica e a funcionalidade pela Medida de Independência Funcional. Resultados: na primeira avaliação, os pacientes não relataram dor. Na avaliação pós-operatória, os pacientes apresentaram uma média de 4,75 ± 1,91 pontos na escala de dor. A funcionalidade passou de 124,29 ± 6,38 no pré-operatório para 97,42 ± 12,73 no pós-operatório. Verificou-se correlação significativa entre dor e funcionalidade. Conclusão: ocorreu aumento da dor e redução da funcionalidade no pós-operatório de cirurgia cardíaca, além de correlação entre as variáveis, demonstrando que uanto maior a intensidade da dor, menor é a independência funcional do paciente.</p><p><br />Descritores: Cirurgia torácica. Medição da dor. Fisioterapia. Reabilitação.</p>
FATORES ASSOCIADOS À QUALIDADE DE VIDA DE INDIVÍDUOS ACOMETIDOS POR DIABETES MELLITUS
Authors: De Oliveira Bruno Gonçalves , Bomfim Eliane Dos Santos , Ribeiro Icaro José  Santos , Carmo Erica Assunção , Boery Rita Narriman Silva de Oliveira , Boery Eduardo Nagib 
Published: January 2018

<p>Objetivo: analisar os fatores associados à qualidade de vida de indivíduos acometidos por diabetes mellitus. Método: estudo epidemiológico, censitário, realizado com 101 indivíduos com o diagnóstico de diabetes tipo 2 atendidos em uma Unidade de Saúde da Família do município de Jequié-BA, Brasil. Foram utilizados os instrumentos de coleta de dados: sociodemográficos, estilo de vida, dados clínicos e o WHOQOL-bref. Resultados: evidenciou-se que as pessoas que fazem uso de álcool e tabaco apresentaram melhor percepção de qualidade de vida no domínio relações sociais. Verificou-se que os indivíduos sobrepeso/obeso apresentaram pior percepção de qualidade de vida no domínio psicológico. Quanto ao tempo diagnóstico, observou-se pior percepção entre os indivíduos com diagnóstico &gt; 5 anos de diabetes mellitus e o domínio relações sociais. Conclusão: o consumo de álcool e tabaco, índice de massa corporal e o tempo diagnóstico da doença interferem na qualidade de vida do indivíduo com diabetes.</p><p><br />Descritores: Diabetes mellitus. Estilo de vida. Qualidade de vida.</p>
Longest Ultra Slim Lateral in the World Drilled with Slim Mud Motor Technology in Kuwait Oil Company Fields SPE
Authors: Alkhaldy Meshal , Alsaadi Dhari , Al-Shuaibi Nawaf , Al-Saffar Maytham , Elafify Ibrahim , Alkubaish Yasser , Moustafa Shady , Mobasher Mohamed , Dalali Hamad Al , Saleh Rashad , Keot Chandan Jyoti , Jokhi Ayomarz Homi , Hasan Said 
Published: January 2018

Abstract
The objective is to increase the production/injection rate of the existing old sick vertical wells that are completed with 7" casing, by extending the reservoir exposure with converting those wells to horizontal producer/injector.
In the initial Field Development stage, many wells were drilled and completed with 7" casing up to the surface. Several of those old wells are lying inactive in North Kuwait which has one of the largest oil reservoirs in the country. In addition, of the limited surface locations in the field combined with the growing anti-collision challenges, feasibility studies were carried out for reviving such sick wells which can't be delivered via limited hole sections; accordingly, an innovative idea introduced to complete those wells with 3 7/8" or 4 1/8" Open Hole Completion. To address the required challenges, 2-7/8" PAC HT DP had been used along with 3 1/8" Motor.
Consequently, DP had to be spaced out every trip to maximize the WOB transfer and mitigate Buckling issues.
This paper provides an overview of 3 7/8" slim horizontal hole by taking the challenge to the next level by drilling the longest 3 7/8" horizontal slim hole in the world with a total footage of 4,565 ft. Total 7-runs had been performed to achieve this lateral length due to the limitation of BHA/LWD Battery life. Thirteen such wells have already been drilled consisting of 7 Injectors and 6 Producers. The Slim Wells are completed as an Injector with 5" seal assembly along with 4 ½" Tubing, or as a Producer with 3 ½" tubing and ESP. All wells (both Injectors and Producers) have yielded results commensurate to the potential of any new conventional horizontal well. Consequently, Slim Hole Horizontal wells were successfully drilled in Kuwait Oil Company fields. As such, the campaign of reviving the sick wells helped KOC to achieve higher return on investment in mature assets through minimizing top hole construction cost and time. The success has opened up new avenues for KOC.
Re-entry wells helped to achieve higher return on investment in mature assets through minimizing top hole construction cost and have been drilled at 1/3rd the cost of a new well. It also accelerates time to oil production from existing wells and has minimized the prevailing location constraints. KOC has launched an aggressive plan to complete more such sick wells.
11 ERD Wells Engineered to Deliver 50+ Field Records in Qarn Alam Cluster, Sultanate of Oman
Authors: El Hawy Ahmed , Picha Mahesh Shrichand , Soliman Fathy , Haeser Patrick A , Al Hadhrami Moosa , Al Kindi Adil , Eljenni Mounir , Habsi Is'Haq , Al Busaidi Ibrahim , Al Busaidi Adil Zahran , Bazara Magdi , Omara Ahmed Sadig 
Published: January 2018

Abstract
As conventional drilling learning curves mature from drilling simple vertical wells to deviated wells to complex multi-lateral horizontal wells, the boundaries needed to be broken to reach much deeper depths rather than consuming the time in drilling multiple shorter laterals.
Horizontal ERD wells in Qarn Alam cluster were planned to be drilled in four sections where the 17.5-in section is drilled vertically followed by a deviated 12.25-in section and continued by landing in 8.5-in section and finally the 6.125-in horizontal lateral.
Many attempts of performance improvement initiatives were executed over many years however there were always flaws and inconsistency in drilling performance delivery. As the need of ERD grew, a detailed offset wells analysis had to be performed where all the deficiencies and issues had to be pin pointed, RCA (Root Cause Analysis) had to be performed and plans for success had to be laid out.
From challenges achieving required dog legs in the top sections with increased risks of axial and lateral vibrations, to the difficulties faced in the landing section drilling through unconsolidated and reactive shales, to the difficulties transferring weight to the bit at deeper depths in the horizontal laterals drilling highly porous zones of sticky limestones resulting in severe torsional vibrations. A new approach of drilling had to be executed with a renovated set of drilling parameters envelopes, revised trajectory designs, re-engineered BHA designs, right choice of fit for purpose bits and effective real-time performance monitoring.