bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of an UPLC‐Q/TOF‐MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study
Authors: Geng Cong , Wang Chun‐hong , Hu Hong , Gao Xiao‐ping , Gong Ai‐hua , Lin Ying‐wei , Fan Xiu‐shuang , Li Heng , Yin Jian‐yuan 
Published: October 2016

AbstractNeopanaxadiol (NPD), the main panaxadiol constituent of Panax ginseng C. A. Meyer (Araliaceae), has been regarded as the active component for the treatment of Alzheimer's disease. However, few references are available about pharmacokinetic evaluation for NPD. Accordingly, a rapid and sensitive method for quantitative analysis of NPD in beagle dog plasma based on ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry was developed and validated. Analytes were extracted from plasma by liquid–liquid extraction and chromatographic separation was achieved on an Agilent Zorbax Stable Bond C18 column. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions both at m/z 461.4 → 425.4 for NPD and internal standard of panaxadiol. All validation parameters, such as lower limit of quantitation, linearity, specificity, precision, accuracy, extraction recovery, matrix effect and stability, were within acceptable ranges and the method was appropriate for multitude sample determination. After oral intake, NPD was slowly absorbed and eliminated from circulatory blood system and corresponding plasma exposure was low. Application of this quantitative method will yield the first pharmacokinetic profile after oral administration of NPD to beagle dog. The information obtained here will be useful to understand the pharmacological effects of NPD.
Epidemiology of complementary and alternative medicine therapy use in allogeneic hematopoietic stem cell transplant survivorship patients in Australia
Authors: Lindsay Julian , Kabir Masrura , Gilroy Nicole , Dyer Gemma , Brice Lisa , Moore John , Greenwood Matthew , Hertzberg Mark , Gottlieb David , Larsen Stephen R. , Hogg Megan , Brown Louisa , Huang Gillian , Tan Jeff , Ward Christopher , Kerridge Ian 
Published: October 2016

AbstractIn addition to prescribed conventional medicines, many allogeneic hematopoietic stem cell transplant (HSCT) survivors also use complementary and alternative medical therapies (CAM), however, the frequency and types of CAMs used by allogeneic HSCT survivors remain unclear. Study participants were adults who had undergone an allogeneic HSCT between 1st January 2000 and 31st December 2012. Participants completed a 402‐item questionnaire regarding the use of CAM, medical complications, specialist referrals, medications and therapies, infections, vaccinations, cancer screening, lifestyle, and occupational issues and relationship status following stem cell transplantation. A total of 1475 allogeneic HSCT were performed in the study period. Of the 669 recipients known to be alive at study sampling, 583 were contactable and were sent study packs. Of 432 participants who returned the completed survey (66% of total eligible, 76% of those contacted), 239 (54.1%) HSCT survivors used at least one form of CAM. These included dietary modification (13.6%), vitamin therapy (30%), spiritual or mind–body therapy (17.2%), herbal supplements (13.5%), manipulative and body‐based therapies (26%), Chinese medicine (3.5%), reiki (3%), and homeopathy (3%). These results definitively demonstrate that a large proportion of HSCT survivors are using one or more form of CAM therapy. Given the potential benefits demonstrated by small studies of specific CAM therapies in this patient group, as well as clearly documented therapies with no benefit or even toxicity, this result shows there is a large unmet need for additional studies to ascertain efficacy and safety of CAM therapies in this growing population.
The role of FilGAP, a Rac‐specific Rho‐GTPase‐activating protein, in tumor progression and behavior of astrocytomas
Authors: Hara Atsuko , Hashimura Miki , Tsutsumi Koji , Akiya Masashi , Inukai Madoka , Ohta Yasutaka , Saegusa Makoto 
Published: October 2016

AbstractFilGAP, a Rac‐specific Rho‐GTPase‐activating protein (GAP), acts as a mediator of Rho/ROCK‐dependent amoeboid movement, and its knockdown results in Rac‐driven mesenchymal morphology. Herein, we focused on the possible roles of FilGAP expression in astrocytomas. In clinical samples, FilGAP expression was significantly increased in grade (G) II astrocytomas as compared to normal astrocytes, but its expression strongly decreased in a grade‐dependent manner, and was positively associated with isocitrate dehydrogenase 1 (IDH1) mutations and inversely to cytoplasmic Rac1. Patients with astrocytoma showing a high FilGAP score had favorable overall survival as compared to the low score patients. Multivariate Cox regression analysis also showed that a high FilGAP score was a significant and independent favorable prognostic factor. Moreover, patients with high FilGAP score and IDH1 mutant‐type astrocytomas had significantly the best Overall survival (OS) and Progression‐free survival (PFS), in contrast to the patients with low FilGAP score and wild‐type IDH1 tumors who had the worst prognosis. In GIV tumors (GBM: glioblastomas), elongated tumor cells with low FilGAP expression were frequently observed in tumor core lesions, whereas the rounded cells with abundant expression were found in the peripheral areas adjacent to non‐neoplastic brain tissues. In an astrocytoma cell line, suppression of endogenous FilGAP expression by siRNAs caused an increased proportion of mesenchymal elongated cells, probably through increased Rac1 activity. These findings suggest that FilGAP, as well as IDH1 status, may be useful for predicting the behavior of astrocytomas. In addition, the FilGAP/Rac1 axis may serve as an important regulator of tumor progression in GBMs, probably through alteration of cell morphology.
Upregulation of LncRNA‐HIT promotes migration and invasion of non‐small cell lung cancer cells by association with ZEB1
Authors: Jia Xiaojing , Wang Zhicheng , Qiu Ling , Yang Yanming , Wang Yunlong , Chen Zhishen , Liu Zhongshan , Yu Lei 
Published: October 2016

AbstractLung cancer is the most common solid tumor and the leading cause of cancer‐related mortality worldwide. Non‐small cell lung cancer (NSCLC) accounts for approximately 80% of all lung cancer cases. The main reason of lung cancer‐related deaths is due to tumor metastasis. But, the mechanisms of NSCLC metastasis remains poorly understood. LncRNAs play pivotal roles in multiple biological processes. LncRNA‐HIT (HOXA transcript induced by TGFβ) was recently identified. LncRNA‐HIT promotes cell migration, invasion, tumor growth, and metastasis. However, the detailed role of lncRNA‐HIT in NSCLC remains unknown. In this study, for the first time, we revealed a novel role of lncRNA‐HIT in the migration and invasion of NSCLC cells. The expression of lncRNA‐HIT was significantly upregulated in NSCLC tissues and cell lines, and the expression level of lncRNA‐HIT correlates with advanced disease stage and predicts unfavorable prognosis of NSCLC patients. Functional assays demonstrated that lncRNA‐HIT markedly increased the ability of NSCLC cells to migrate and invade. Furthermore, the molecular mechanism by which lncRNA‐HIT affects NSCLC cells was associated with regulation of ZEB1 stability. LncRNA‐HIT functions as a prometastasis oncogene by directly associating with ZEB1 to regulate NSCLC. The interaction of lncRNA‐HIT and ZEB1 may be a potential target for NSCLC therapy.
Recognition: An exploration of the processes of counselling and poetry
Authors: Dorman Fingal 
Published: October 2016

AbstractBackgroundThis article recognises an affinity between the process of counselling and the creative process of writing poetry. It is an exploration into the nature of this connection as a way to get closer to the elusive internal processes of counselling.MethodI use writing as a method of inquiry, acknowledging writing as a generative process that can make unexpected connections and go deeply into the nature of experience. Influenced by autoethnography, this writing comprises personal accounts, writing into creative and therapeutic processes, followed by an interpretation of those accounts, drawing on the literatures of poetry and counselling.FindingsThe process of staying with uncertainty is a key feature of both therapy and poetry. I identify an unclear internal sense of s‘something’ as a key part of this experience. Listening and giving credit to this allows it to develop in new directions, in a process in which play is important. The ambiguity of language opens up the unknown parts of experience rather than representing pre‐existing feelings. This space for the unexpected gives potential for creativity or a therapeutic shift.Implications for practiceThis research gets close to the nuanced and unclear internal and interpersonal space of counselling. It emphasises the need for space for ‘play’ and the unexpected. It makes links and opens connections between two different literatures and fields of practice. I invite responses from others, as a potential stimulus for dialogue about the internal processes of practice and their meaning in context.
Effect of Methyl Group on Acyclic Serinol Scaffold for Tethering Dyes on the DNA Duplex Stability
Authors: Murayama Keiji , Asanuma Hiroyuki 
Published: October 2016

AbstractAcyclic serinol derivatives are useful scaffolds for tethering dyes within DNA duplexes. Here we synthesised an inverse l‐threoninol (il‐threoninol) scaffold and compared its effect on DNA duplex stability to other acyclic artificial nucleic acid scaffolds that are based on d‐threoninol, l‐threoninol, and serinol. When planar trans‐azobenzene was incorporated into the DNA duplex through a single bulge‐like motif (the wedge), the il‐threoninol scaffold stabilised the duplex most efficiently. When scaffolds were incorporated in complementary positions (dimer motif) or in three adjacent positions (cluster motif), d‐threoninol was the most stabilising. CD spectra indicated that the effect of scaffold on the duplex stability was closely related to the winding induced by each scaffold. When trans‐azobenzene was photo‐isomerised to non‐planar cis‐azobenzene, il‐threoninol destabilised the duplex most strongly, irrespective of the number of artificial residues incorporated. The properties of the il‐threoninol scaffold make it a useful tether for dyes or other functionalities.
Noninvasive management for iatrogenic splenic injury caused by chest tube insertion: a case report
Authors: Ohtaka Kazuto , Hase Ryunosuke , Chiba Ryohei , Miyasaka Mamoru , Sato Shoki , Shoji Yasuhito , Ichimura Tatsunosuke , Senmaru Naoto , Kaga Kichizo , Matsui Yoshiro 
Published: October 2016

Key Clinical MessageSplenic injury is one of the most critical complications of chest tube insertion and often requires invasive emergency management. However, noninvasive management such as delayed removal of the malpositioned tube may be considered for a stable patient without severe adverse event.
The Immobilization of a Transfer Hydrogenation Catalyst on Colloidal Particles
Authors: van Ravensteijn Bas G. P. , Schild Dirk‐Jan , Kegel Willem K. , Klein Gebbink Robertus J. M. 
Published: October 2016

AbstractIn this paper, we report a new synthetic procedure to immobilize a transfer hydrogenation catalyst on the surface of colloidal polystyrene particles. Using supports of colloidal dimensions allows for combining a relatively high surface area for catalyst binding, mobility of the catalyst, and facile recovery by centrifugation or sedimentation. The immobilization procedure relies on the covalent attachment of terpyridine moieties on the particle surface. These immobilized terpyridines are subsequently employed as colloidal ligands, which participate in the formation of an active ruthenium‐based transfer hydrogenation catalyst. The resulting functional colloidal particles successfully catalyze the transfer hydrogenation of acetophenone with 2‐propanol as the hydrogen donor. Thorough analysis of the chemical composition of the colloidal surface led to the determination of the catalyst loading per particle. This enabled us to conduct reference hydrogenations with equal concentrations of the homogeneous transfer hydrogenation catalyst to probe the effect of the immobilization procedure on the catalytic activity. Despite a decrease in transfer hydrogenation activity, full acetophenone conversion is still achievable within 24 h. Preliminary experiments show that the catalytic colloids are recyclable without significant loss of transfer hydrogenation activity.
Palladium‐Catalyzed Carbonylative Synthesis of 3‐Methyleneisoindolin‐1‐ones from Ketimines with Hexacarbonylmolybdenum(0) as the Carbon Monoxide Source
Authors: Wang Zechao , Zhu Fengxiang , Li Yahui , Wu Xiao‐Feng 
Published: October 2016

AbstractAn interesting procedure for the palladium‐catalyzed carbonylative synthesis of 3‐methyleneisoindolin‐1‐ones from ketimines was established. By using Mo(CO)6 (0.3 equiv.) as the solid CO source and through C(sp2)−H bond activation, the desired 3‐methyleneisoindolin‐1‐ones were isolated in moderate to good yields.
Enzymatic versus Electrocatalytic Oxidation of NADH at Carbon‐Nanotube Electrodes Modified with Glucose Dehydrogenases: Application in a Bucky‐Paper‐Based Glucose Enzymatic Fuel Cell
Authors: Lalaoui Noémie , Means Nicolas , Walgama Charuksha , Le Goff Alan , Holzinger Michael , Krishnan Sadagopan , Cosnier Serge 
Published: October 2016

AbstractIn this work, we have designed and compared functionalized carbon‐nanotube‐based electrodes for the electrocatalytic oxidation of NADH. We show that oxidation of NADH at neutral pH values can be performed by pristine multi‐walled carbon nanotubes (MWCNT) and directly wired diaphorase or ruthenium phenanthrolinequinone molecular catalysts. Glucose dehydrogenase was immobilized on these MWCNT electrodes by an activated‐ester‐modified pyrene linker. Glucose‐oxidizing bioelectrodes were compared and integrated into a bucky‐paper‐based glucose/O2 biofuel cell, using a bilirubin‐oxidase‐modified bucky‐paper cathode. The best configuration was obtained using a NADH‐oxidizing molecular catalyst at the anode. The biofuel cell exhibits an open‐circuit voltage of 0.62 V and delivers a power output of 0.47 mW cm−2.
Mechanism and Applications of the Photoredox Catalytic Coupling of Benzyl Bromides
Authors: Park Gyurim , Yi Seung Yeon , Jung Jaehun , Cho Eun Jin , You Youngmin 
Published: October 2016

AbstractThe photoredox catalytic coupling of halomethyl arenes to bibenzyl derivatives has been demonstrated. The catalytic protocol employed the Hantzsch ester, potassium phosphate, and a photoactive cyclometalated IrIII complex catalyst. A photochemical quantum yield as high as 20 % was obtained. The catalytic mechanism was investigated in detail by performing photophysical and electrochemical measurements, as well as by quantum chemical calculations. The results suggest that two‐electron mediation might be responsible for the improved photon economy. The reaction protocol was compatible with halomethyl arenes that contain a variety of functional groups. Finally, the synthetic utility of our protocol was demonstrated by the preparation of a natural dihydrostilbenoid, brittonin A.
Key Non‐Metal Ingredients for Cu‐catalyzed “Click” Reactions in Glycerol: Nanoparticles as Efficient Forwarders
Authors: Rodríguez‐Rodríguez Marta , Llanes Patricia , Pradel Christian , Pericàs Miquel A. , Gómez Montserrat 
Published: October 2016

AbstractThe effect of long‐alkyl‐chain amines in CuI‐assisted azide–alkyne cycloadditions of terminal alkynes with organic azides in glycerol and other environmentally benign solvents (water, ethanol) has been examined. The presence of these additives favors the in situ formation of CuI‐based nanoparticles and results in an increase of the catalytic reactivity. In glycerol, liquid‐phase transmission electron microscopy (TEM) analyses, enabled by the negligible vapor pressure of this solvent, proved that CuI nanoparticles are responsible for the observed catalytic activity. The wide variety of alkynes and azides of which this effect has been investigated (14 combinations) confirms the role played by these additives in Cu‐catalyzed Huisgen cycloadditions.
Metal‐Free Benzylic C−H Amination via Electrochemically Generated Benzylaminosulfonium Ions
Authors: Hayashi Ryutaro , Shimizu Akihiro , Song Yetao , Ashikari Yosuke , Nokami Toshiki , Yoshida Jun‐ichi 
Published: October 2016

AbstractElectrochemical oxidation of toluene derivatives in the presence of N‐tosyldiphenylsulfilimine gave the corresponding benzylaminosulfonium ions, which were treated with tetrabutylammonium iodide under non‐electrolytic conditions to give N‐tosylbenzylamines. The transformation serves as a metal‐ and chemical‐oxidant‐free method for benzylic C−H amination. Because of high oxidation potential of N‐tosyldiphenylsulfilimine the present method can be applied to synthesis of various benzylamines from functionalized toluene derivatives.
Reactions of Trimethylaluminium: Modelling the Chemical Degradation of Synthetic Lubricants
Authors: Slaughter Jonathan , Peel Andrew J. , Wheatley Andrew E. H. 
Published: October 2016

AbstractIn investigating and seeking to mimic the reactivity of trimethylaluminium (TMA) with synthetic, ester‐based lubricating oils, the reaction of methyl propionate 1 was explored with 1, 2 and 3 equivalents of the organoaluminium reagent. Spectroscopic analysis points to the formation of the adduct 1(TMA) accompanied only by the low level 1:1 production of Me2AlOCEtMe2 2 and Me2AlOMe 3 when an equimolar amount of TMA is applied. The deployment of excess TMA favours reaction to give 2 and 3 over 1(TMA) adduct formation and spectroscopy reveals that in hydrocarbon solution substitution product 2 traps unreacted TMA to yield 2(TMA). The 1H NMR spectroscopic observation of two Al−Me signals not attributable to free TMA and in the ratio 1:4 suggests the formation of a previously only postulated, symmetrical metallacycle in Me4Al2(μ2‐Me)(μ2‐OCEtMe2). In the presence of 3, 2(TMA) undergoes thermally induced exchange to yield Me4Al2(μ2‐OMe)(μ2‐OCEtMe2) 4 and TMA. The reaction of methyl phenylacetate 5 with TMA allows isolation of the crystalline product Me2AlOCBnMe2(TMA) 6(TMA), which allows the first observation of the Me4Al2(μ2‐Me)(μ2‐OR) motif in the solid state. Distances of 2.133(3) Å (Al−Mebridging) and 1.951 Å (mean Al−Meterminal) are recorded. The abstraction of TMA from 6(TMA) by the introduction of Et2O has yielded 6, which exists as a dimer.
Claisen–Schmidt condensation: Synthesis of (1S,6R)/(1R,6S)‐2‐oxo‐N,4,6‐triarylcyclohex‐3‐enecarboxamide derivatives with different substituents in H2O/EtOH
Authors: Mousavi Seyyed Rasul 
Published: October 2016

AbstractA simple, green, and direct three‐component condensation of acetophenone, aromatic aldehydes with 3‐oxo‐N‐phenylbutanamide (acetoacetanilide) to generate some novel (1S,6R)/(1R,6S)‐2‐oxo‐N,4,6‐triarylcyclohex‐3‐enecarboxamide derivatives was carried out over K2CO3 (10 mol%) with high efficiency in water/ethanol as green solvent at room temperature. This protocol proceeded via Claisen–Schmidt condensation and Michael addition. The present methodology offers several advantages, such as short reaction time, high yield, more readily available and inexpensive materials, more environmentally friendly, no need for column chromatography, simple work‐up procedure, and the absence of volatile and hazardous organic solvents.
Elektrochrome Fenster mit leitenden Polymeren
Authors: Rendón‐Enríquez Ibeth N. , Tausch Michael W. , Scherf Ullrich 
Published: October 2016

AbstractEs wird der Bau eines kostengünstigen Potentiostats und einer elektrochemischen Zelle beschrieben, die für potentiostatische Abscheidungen von leitenden Polymeren auf FTO‐beschichtete Gläser eingesetzt wurden. Ein literaturbekanntes elektrochromes Fenster wurde hergestellt und mit dem Potentiostat getestet. Darüber hinaus wurde ein neues Fenster mit einer zusätzlichen elektrochemisch abgeschiedenen Polymerschicht konzipiert und erstmalig hergestellt, dessen Elektrochromie ausgeprägter ist als beim Literaturbeispiel. Die dafür notwendigen Chemikalien sind ebenso wie das gesamte Equipment preisgünstig.
The effectiveness of deservingness‐based advertising messages: The role of product knowledge and belief in a just world
Authors: Hafer Carolyn L. , Mantonakis Antonia , Fitzgerald Regan , Bogaert Anthony F. 
Published: October 2016

AbstractAre advertisements that tell consumers they deserve a product effective? Given evidence showing people are motivated to get what they deserve, we argue that for certain consumers this is the case. In two experiments, we manipulated the type of ad participants viewed for a luxury product and then assessed ad effectiveness. We also measured participants’ belief in a just world (BJW) and product knowledge. Deservingness‐based ads were (a) more effective for strong BJW participants, mediated by the degree to which the ads matched participants’ worldview and justified buying the product, and (b) less effective for high knowledge participants. We conclude that deservingness‐based advertising can be successful, depending on consumer characteristics. Copyright © 2016 ASAC. Published by John Wiley & Sons, Ltd.
Hierarchical Bayesian small area estimation for circular data
Authors: Hernandez‐Stumpfhauser Daniel , Breidt F. Jay , Opsomer Jean D. 
Published: October 2016

AbstractWe consider small area estimation for the departure times of recreational anglers along the Atlantic and Gulf coasts of the United States. A Bayesian area‐level Fay–Herriot model is considered to obtain estimates of the departure time distribution functions. The departure distribution functions are modelled as circular distributions plus area‐specific errors. The circular distributions are modelled as projected normal, and a regression model is specified to borrow information across domains. Estimation is conducted through the use of a Hamiltonian Monte Carlo sampler and a projective approach onto the probability simplex.The Canadian Journal of Statistics44: 416–430; 2016 © 2016 Statistical Society of Canada
Structural Simplification of Bedaquiline: the Discovery of 3‐(4‐(N,N‐Dimethylaminomethyl)phenyl)quinoline‐Derived Antitubercular Lead Compounds
Authors: He Chunxian , Preiss Laura , Wang Bin , Fu Lei , Wen Hui , Zhang Xiang , Cui Huaqing , Meier Thomas , Yin Dali 
Published: October 2016

AbstractBedaquiline (BDQ) is a novel and highly potent last‐line antituberculosis drug that was approved by the US FDA in 2013. Owing to its stereo‐structural complexity, chemical synthesis and compound optimization are rather difficult and expensive. This study describes the structural simplification of bedaquiline while preserving antitubercular activity. The compound's structure was split into fragments and reassembled in various combinations while replacing the two chiral carbon atoms with an achiral linkage instead. Four series of analogues were designed; these candidates retained their potent antitubercular activity at sub‐microgram per mL concentrations against both sensitive and multidrug‐resistant (MDR) Mycobacterium tuberculosis strains. Six out of the top nine MIC‐ranked candidates were found to inhibit mycobacterial ATP synthesis activity with IC50 values between 20 and 40 μm, one had IC50>66 μm, and two showed no inhibition, despite their antitubercular activity. These results provide a basis for the development of chemically less complex, lower‐cost bedaquiline derivatives and describe the identification of two derivatives with antitubercular activity against non‐ATP synthase related targets.