bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of seasonality and habitat on the browsing and frugivory preferences of Tapirus terrestris in north-western Amazonia
Authors: Vélez Juliana , Espelta Josep Maria , Rivera Orlando , Armenteras Dolors 
Published: November 2017

Abstract:Herbivore foraging is influenced by spatial and seasonal changes in the production of leaves and fruits. To understand how herbivores respond to these changes, it is necessary to identify their habitat preferences and how they use the vegetation available. In the Neotropical region, one of the largest terrestrial herbivores is the lowland tapir (Tapirus terrestris), a species important for its dual role as browser and seed disperser. The objective of this study was to determine the species and plant components (leaves, fruits) utilized by T. terrestris in different time periods and habitats, in relation to changes in food availability in the north-western Amazon. Tapir diet was established through identification of browsing signs and faecal analysis, from data collected in the field during the months of March, April, August and September of 2015. Plant species availability for browsing was sampled in ten 2 × 50-m transects and fruit productivity was estimated in linear transects (~9 km). We found that T. terrestris mostly consumed vegetative parts, i.e. fibre (70–90%), and to a lesser extent fruits (10–30%). Food consumption was selective and concentrated in habitats with higher availability of preferred plants. When fruit intake increased, selectivity in browsing became more intense and limited to preferred species. This information, coupled with our findings about seasonal differences in browsing vs. frugivory patterns, provides valuable knowledge for understanding how environmental heterogeneity may influence the foraging ecology of the lowland tapir.
Disfluencies signal reference to novel objects for adults but not children
Authors: OWENS Sarah J. , THACKER Justine M. , GRAHAM Susan A. 
Published: November 2017

AbstractSpeech disfluencies can guide the ways in which listeners interpret spoken language. Here, we examined whether three-year-olds, five-year-olds, and adults use filled pauses to anticipate that a speaker is likely to refer to a novel object. Across three experiments, participants were presented with pairs of novel and familiar objects and heard a speaker refer to one of the objects using a fluent (“Look at the ball/lep!”) or disfluent (“Look at thee uh ball/lep!”) expression. The salience of the speaker's unfamiliarity with the novel referents, and the way in which the speaker referred to the novel referents (i.e., a noun vs. a description) varied across experiments. Three- and five-year-olds successfully identified familiar and novel targets, but only adults’ looking patterns reflected increased looks to novel objects in the presence of a disfluency. Together, these findings demonstrate that adults, but not young children, use filled pauses to anticipate reference to novel objects.
DISPARATE LESSONS
Authors: Reyes Daisy Verduzco 
Published: November 2017

AbstractThis paper compares the identity-formation processes of Latino students in three different college contexts (a liberal arts college, a research university, and a regional public university). Drawing on ethnographic observations, in-depth interviews, and surveys of members of Latino student organizations, I chart the distinct ways in which Latino students interact with one another and arrive at particular ethnic identities on different campuses. By applying ethnoracialization theory to mesolevel settings, I examine how students respond to external ascription as they co-construct and negotiate their ethnic-racial understandings. I identify three different patterns by which students deploy panethnic boundaries, specifically, as they adopt and define identity labels: inclusive Latino identification signifying solidarity above all, qualified Latino identification mediated through specific organizational membership, and the rejection of panethnic identities. I consider how the organizational context of each campus provides a distinct racial climate that mediates student interactions and potentially shapes the disparate identity outcomes that result. The findings suggest that, beyond providing academic experiences, colleges also provide Latino students with disparate lessons regarding who they are and where they fit in the ethnoracial hierarchy.
From Traction to Friction in Thailand: The Emerging Southeast Asian DevelopmentProblematique
Authors: Rigg Jonathan 
Published: November 2017

AbstractUsing the notion of an emerging developmentproblematiquein Southeast Asia, this paper uses Thailand to argue that the development challenge is not being solved by economic growth, but reworked. Furthermore, while the policy aims of the early development era could be quite easily identified, measured and addressed, those that have emerged since the Millennium have proved to be more difficult to specify and less amenable to resolution. Drawing on village studies in northeast Thailand, this paper argues that the social adjustments and perturbations engendered by development have created second-order, often more intractable problems and challenges. In this way, the development traction of the early development decades has frequently turned into friction, with the state and its planning and development agencies increasingly struggling to meet both their own objectives and the aspirations of those for whom development was intended. It is also argued that Thailand'sproblematiqueis reflected in three emerging gaps: a development gap between what the Thai government is attempting to achieve and the willingness of the Thai population to join in that journey; a political gap reflected in Thailand's Red Shirt/Yellow Shirt conflict; and an aspirational gap between what has been achieved and what is aspired to.
Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury
Authors: Feng Zunyong , Qi Shimei , Zhang Yue , Qi Zhilin , Yan Liang , Zhou Jing , He Fang , Li Qianqian , Yang Yanyan , Chen Qun , Xiao Shi , Li Qiang , Chen Yang , Zhang Yao 
Published: November 2017

AbstractMicroRNA (miRNA) mediates RNA interference to regulate a variety of innate immune processes, but how miRNAs coordinate the mechanisms underlying acute lung injury/acute respiratory distress syndrome (ALI/ARDS) in patients with pulmonary inflammatory injury is still unknown. In this study, we demonstrated that miR-223 limits the number of Ly6G+ neutrophils and inhibits the activity of the NLRP3 inflammasome to alleviate ALI induced by mitochondrial damage-associated molecular patterns (DAMPs) (MTDs). miR-223 expression is increased in the lungs of MTD-induced mice or ARDS patients following trauma/transfusion or following the physiological remission of ALI/ARDS. miR-223−/+ mice exhibited more severe ALI and cytokine dysregulation. Other studies have shown that MTD-induced increases in miR-223 expression are mainly contributed by Ly6G+ neutrophils from the haematopoietic system. miR-223 blocks bone marrow-derived Ly6G+ neutrophil differentiation and inhibits peripheral cytokine release. In addition, MTD-induced miR-223 expression activates a negative feedback pathway that targets the inhibition of NLRP3 expression and IL-1β release; therefore, miR-223 deficiency can lead to the sustained activation of NLRP3-IL-1β. Finally, elimination of peripheral Ly6G+ neutrophils and pharmacological blockade of the miR-223–NLRP3–IL-1β signalling axis could alleviate MTD-induced ALI. In summary, miR-223 is essential for regulating the pathogenesis of DAMP-induced ALI.
A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection
Authors: Hu Jiaming , Sheng Yan , Kwak Kwang Joo , Shi Junfeng , Yu Bohao , Lee L. James 
Published: November 2017

AbstractDetection of extracellular vesicle (EV)-associated RNAs with low expression levels in early-stage cancer remains a challenge and is highly valuable. Here, we report a nanoparticle-based biochip that could capture circulating EVs without isolation, brighten encapsulated RNAs, and amplify fluorescence signals in situ in a single step. We confine catalyzed hairpin DNA circuit (CHDC) in cationic lipid-polymer hybrid nanoparticles (LPHNs) that are tethered on a chip. LPHN features a core-shell-corona structure that facilitates the transfer and mixing of CHDC with EV-associated RNAs when forming the LPHN–EV nanocomplex. CHDC is triggered upon target RNA binding and quickly generate amplified signals. The signal amplification efficiency of LPHN–CHDC is demonstrated in artificial EVs, cancer cells, and cancer cell-derived EVs. We show that LPHN–CHDC biochip with signal amplification capability could selectively and sensitively identify low expression glypican-1 mRNA in serum EVs, distinguishing patients with early- and late-stage pancreatic cancer from healthy donors and patients with benign pancreatic disease.
Fabrication and Verification of Conjugated AuNP-Antibody Nanoprobe for Sensitivity Improvement in Electrochemical Biosensors
Authors: Khashayar Patricia , Amoabediny Ghassem , Larijani Bagher , Hosseini Morteza , Vanfleteren Jan 
Published: November 2017

AbstractThis study was designed to obtain covalently coupled conjugates as means for achieving higher stability and better coverage of the AuNPs by antibodies on the particle surface suitable for sensor performance enhancement. Starting by using a modified protocol, colloid gold solution, with mean AuNP core size of ~6 nm was synthesized. The protocol used for conjugation of AuNPs to osteocalcin antibody in this study relies on covalent and electrostatic attractions between constituents. Varieties of conjugates with varying combinations of crosslinkers and different concentrations were successfully synthesized. The obtained products were characterized and their properties were studied to determine the best candidate in sense of antibody - antigen reactivity. Using AuNP-GSH-NHS-Ab combination (1:1:1), the tertiary structure of the protein was maintained and thus the antibody remained functional in the future steps. This one-pot method provided a simple method for covalently coupling antibodies on the particle surface while keeping their functionality intact. The AuNP content of the solution also accelerated electron transfer rate and thus amplifies the detection signal. With the developed and discussed technique herein, a simple solution is modeled to be used for measuring serum levels of biomarkers in single and/or multiplexed sensor systems.
Full-wave modeling of broadband near field scanning microwave microscopy
Authors: Wu Bi-Yi , Sheng Xin-Qing , Fabregas Rene , Hao Yang 
Published: November 2017

AbstractA three-dimensional finite element numerical modeling for the scanning microwave microscopy (SMM) setup is applied to study the full-wave quantification of the local material properties of samples. The modeling takes into account the radiation and scattering losses of the nano-sized probe neglected in previous models based on low-frequency assumptions. The scanning techniques of approach curves and constant height are implemented. In addition, we conclude that the SMM has the potential for use as a broadband dielectric spectroscopy operating at higher frequencies up to THz. The results demonstrate the accuracy of previous models. We draw conclusions in light of the experimental results.
Hyperoxidation of ether-linked phospholipids accelerates neutrophil extracellular trap formation
Authors: Yotsumoto Satoshi , Muroi Yuito , Chiba Tatsuya , Ohmura Rio , Yoneyama Maki , Magarisawa Megumi , Dodo Kosuke , Terayama Naoki , Sodeoka Mikiko , Aoyagi Ryohei , Arita Makoto , Arakawa Satoko , Shimizu Shigeomi , Tanaka Masato 
Published: November 2017

AbstractBecause neutrophil extracellular trap (NET) formation is involved in the pathology of a wide variety of diseases, NET-regulating compounds are expected to be useful for the therapies of these diseases. In this study, we identified sulfasalazine (SSZ) as a potent enhancer of NET formation bothin vitroandin vivo. Although SSZ did not increase the amount of ROS generated, it accelerated the generation of ether-linked oxidized phospholipids, such as PE (18;1e/15-HETE) and PC (16;0e/13-HODE). Trolox, but not 2-ME, effectively suppressed lipid oxidation and NET formation that were induced by SSZ. SSZ is known as a potent inducer of ferroptosis in cancer cells by inhibiting xCT, a component of the cystine transporter. However, we found that SSZ accelerated NET formation in an xCT-independent manner. Structure-activity relationship studies revealed that the sulfapyridine moiety of SSZ plays a central role in enhancing NET formation. Furthermore, we found that two additional sulfonamide and sulfone derivatives possess NET-inducing activity by accelerating lipid oxidation. These results indicate that the hyperoxidation of ether-linked phospholipids is a key mechanism for accelerating NET formation.
Influence of experimental parameters on the laser heating of an optical trap
Authors: Català Frederic , Marsà Ferran , Montes-Usategui Mario , Farré Arnau , Martín-Badosa Estela 
Published: November 2017

AbstractIn optical tweezers, heating of the sample due to absorption of the laser light is a major concern as temperature plays an important role at microscopic scale. A popular rule of thumb is to consider that, at the typical wavelength of 1064 nm, the focused laser induces a heating rate of B = 1 °C/100 mW. We analysed this effect under different routine experimental conditions and found a remarkable variability in the temperature increase. Importantly, we determined that temperature can easily rise by as much as 4 °C at a relatively low power of 100 mW, for dielectric, non-absorbing particles with certain sets of specific, but common, parameters. Heating was determined from measurements of light momentum changes under drag forces at different powers, which proved to provide precise and robust results in watery buffers. We contrasted the experiments with computer simulations and obtained good agreement. These results suggest that this remarkable heating could be responsible for changes in the sample under study and could lead to serious damage of live specimens. It is therefore advisable to determine the temperature increase in each specific experiment and avoid the use of a universal rule that could inadvertently lead to critical changes in the sample.
Temporal depolarization of mitochondria during M phase
Authors: Hirusaki Kotoe , Yokoyama Kaho , Cho Kyunghak , Ohta Yoshihiro 
Published: November 2017

AbstractMitochondrial activity in cells must be tightly controlled in response to changes in intracellular circumstances. Despite drastic changes in intracellular conditions and mitochondrial morphology, it is not clear how mitochondrial activity is controlled during M phase of the cell cycle. Here, we show that mitochondrial activity is drastically changed during M phase. Mitochondrial membrane potential changed during M phase progression. Mitochondria were polarized until metaphase to the same extent as mitochondria in interphase cells, but were depolarized at around telophase and cytokinesis. After cytokinesis, mitochondrial membrane potential was recovered. In addition, the generation of superoxide anions in mitochondria was significantly reduced at metaphase even in the presence of antimycin A, an inhibitor of complex III. These results suggest that the electron supply to the mitochondrial electron transfer chain is suppressed during M phase. This suppression might decrease the reactive oxygen species generated by the fragmentation of mitochondria during M phase.
Hyperpolarized [1-13C]-acetate Renal Metabolic Clearance Rate Mapping
Authors: Mikkelsen Emmeli F. R. , Mariager Christian Østergaard , Nørlinger Thomas , Qi Haiyun , Schulte Rolf F. , Jakobsen Steen , Frøkiær Jørgen , Pedersen Michael , Stødkilde-Jørgensen Hans , Laustsen Christoffer 
Published: November 2017

Abstract11C-acetate is a positron emission tomography (PET) tracer of oxidative metabolism, whereas hyperpolarized 13C-acetate can be used in magnetic resonance imaging (MRI) for investigating specific metabolic processes. The aims of this study were to examine if the kinetic formalism of 11C-acetate PET in the kidneys is comparable to that of 13C-acetate MRI, and to compare the dynamic metabolic information of hyperpolarized 13C-acetate MRI with that obtained with 11C-acetate PET. Rats were examined with dynamic hyperpolarized 13C-acetate MRI or 11C-acetate PET before and after intravenous injection of furosemide, a loop diuretic known to alter both the hemodynamics and oxygen consumption in the kidney. The metabolic clearance rates (MCR) were estimated and compared between the two modalities experimentally in vivo and in simulations. There was a clear dependency on the mean transit time and MCR for both 13C-acetate and 11C-acetate following furosemide administration, while no dependencies on the apparent renal perfusion were observed. This study demonstrated that hyperpolarized 13C-acetate MRI is feasible for measurements of the intrarenal energetic demand via the MCR, and that the quantitative measures are correlated with those measured by 11C-acetate PET, even though the temporal window is more than 30 times longer with 11C-acetate.
Effect of maternal glycemia and weight status on offspring birth measures and BMI-z among Chinese population in the first year
Authors: Huang Yilin , Yin Baoming , Liang Xiaohong , Mei Hong , Lu Hongyan , Xie Shuixian , Bei Weihong , Mei Wenhua , Zhang Jianduan 
Published: November 2017

AbstractTo investigate the effects of maternal fasting plasma glucose (FPG) and pre-pregnancy weight status (PPWS) on offspring birth measures and body mass index z-score (BMI-z) in the first year, we conducted a prospective study of 1,096 mother-infant dyads in Guangdong, China, 2014–2015. Multivariate logistic regression was used to test independent/interaction associations of maternal FPG and PPWS with macrosomia/large for gestational age (LGA). Association of PPWS and FPG with offspring BMI-z in the first year was assessed by the linear mixed effects models. For each 1-mmol/L increase in FPG, the risk of macrosomia and LGA was elevated by 2.74 and 2.01 (95% CI: 1.85, 7.60 and 1.54, 5.88), respectively. No main effect of PPWS or interaction association of FPG and PPWS on macrosomia/LGA was observed (P > 0.05). A relation between maternal FPG and PPWS was detected (P < 0.05). Infants of Q5 FPG mothers, those who were born to OWO mothers, had a 0.35 increase in the BMI-z (95% CI: 0.16, 0.55) compared with infants of NW mothers. In conclusion, maternal FPG is positively associated with macrosomia/LGA. Maternal PPWS and FPG considerably interacted for the association with the risk of offspring high BMI-z in the first year.
Tuning the Composition and Structure of Amorphous Molybdenum Sulfide/Carbon Black Nanocomposites by Radiation Technique for Highly Efficient Hydrogen Evolution
Authors: Cao Pengfei , Peng Jing , Liu Siqi , Cui Yu , Hu Yang , Chen Bo , Li Jiuqiang , Zhai Maolin 
Published: November 2017

AbstractAmorphous molybdenum sulfide/carbon black (MoSx/C) nanocomposites are synthesized by a facile one-step γ-ray radiation induced reduction process. Amorphous MoSx shows better intrinsic activity than crystalline MoS2. And the composition and amorphous structure of MoSx could be expediently tuned by absorbed dose for excellent catalytic activity. Meanwhile, the addition of carbon black leads to a significant decrease of charge transfer resistance and increase of active sites of MoSx/C composite. Consequently, MoSx/C nanocomposite shows Pt-like catalytic activity towards hydrogen evolution reaction (HER), which requires an onset over potential of 40 mV and over potential of 76 mV to achieve a current density of 10 mA cm−2, and the corresponding Tafel slope is 48 mV decade−1. After 6000 CV cycles, the catalytic activity of MoSx/C shows no obvious decrease. However, when platinum (Pt) foil is used as counter electrode, MoSx/C composite show better catalytic activity abnormally after long-term cycling tests. The dissolution of Pt was observed in HER and the Pt dissolution mechanism is elucidated by further analyzing the surface composition of after-cycling electrodes, which offers highly valuable guidelines for using Pt electrode in HER.
Early vaccination protects against childhood leukemia: A systematic review and meta-analysis
Authors: Morra Mostafa Ebraheem , Kien Nguyen Dang , Elmaraezy Ahmed , Abdelaziz Omar Ayman M. , Elsayed Ahmed Lotfy , Halhouli Oday , Montasr Ahmed Mosaad , Vu Tran Le-Huy , Ho Chau , Foly Amr Sayed , Phi Anh Phan , Abdullah Wessam Magdy , Mikhail Marina , Milne Elizabeth , Hirayama Kenji , Huy Nguyen Tien 
Published: November 2017

AbstractLeukemia is the most commonly diagnosed childhood cancer, although its etiology is still largely unknown. Growing evidence supports a role for infection in the etiology of acute lymphocytic leukemia (ALL), and the involvement of the immune system suggests that vaccination may also play a role. However, the findings presented in the published literature are inconsistent. Therefore, we conducted a PRISMA systematic review and meta-analysis. 14 studies were identified and meta-analyzed. Vaccinations studied comprised Bacillus Calmette-Guérin (BCG) vaccine, Triple vaccine, Hepatitis B vaccine (HBV), Polio, Measles, Rubella, Mumps, trivalent MMR vaccine and Haemophilus influenza type B (HiB) vaccine. We observed a protective association between any vaccination in the first year of life and risk of childhood leukemia (summary odds ratio (OR) 0.58 [95% confidence interval (CI) 0.36–0.91]). When individual vaccines were analysed, some evidence of an association was seen only for BCG (summary OR 0.73 [95% CI 0.50–1.08]). In conclusion, early vaccination appears to be associated with a reduced risk of childhood leukemia. This finding may be underpinned by the association observed for BCG. Given the relatively imprecise nature of the results of this meta-analysis, our findings should be interpreted cautiously and replicated in future studies.
ZIKV infection regulates inflammasomes pathway for replication in monocytes
Authors: Khaiboullina S. F. , Uppal T. , Sarkar R. , Gorzalski A. , St Jeor S. , Verma S. C. 
Published: November 2017

AbstractZIKV causes microcephaly by crossing the placental barrier, however, the mechanism of trans-placental dissemination of ZIKV remains unknown. Here, we sought to determine whether monocytes, which can cross tissue barriers, assist ZIKV dissemination to the fetus. We determined this by infecting monocytes with two strains of ZIKV: South American (PRVABC59) and Nigerian (IBH30656) and analyzing viral replication. We found that ZIKV infects and replicates in monocytes and macrophages, which results in the modulation of a large number of cellular genes. Analysis of these genes identified multiple pathways including inflammasome to be targeted by ZIKV, which was confirmed by analyzing the transcript levels of the proteins of inflammasome pathways, NLRP3, ASC, caspase 1, IL-1 and IL-18. Interestingly, IFNα and the IFN inducible gene, MxA were not enhanced, suggesting prevention of innate antiviral defense by ZIKV. Also, inhibition of inflammasome led to an increased transcriptional activity of IFNα, MxA and CXCL10. Based on these results we suggest that ZIKV transcription is regulated by inflammasomes.