bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beta‐2 microglobulin as a significant prognostic factor and a new risk model for patients with diffuse large B‐cell lymphoma
Authors: Kanemasa Yusuke , Shimoyama Tatsu , Sasaki Yuki , Tamura Miho , Sawada Takeshi , Omuro Yasushi , Hishima Tsunekazu , Maeda Yoshiharu 
Published: May 2016

AbstractPrevious reports have evaluated the prognostic value of serum beta‐2 microglobulin (B2MG) level in patients with non‐Hodgkin lymphoma. However, its role in predicting clinical outcome of patients with diffuse large B‐cell lymphoma (DLBCL) in the rituximab era has not been extensively investigated. Here, we evaluated the prognostic value of B2MG and proposed a new prognostic model including B2MG for patients with DLBCL. A total of 274 patients with newly diagnosed de novo DLBCL were retrospectively analyzed. We defined the best cutoff value as 3.2 mg/L by using a receiver operating characteristic curve. Patients with a B2MG level ≥3.2 mg/L had significantly lower overall survival (OS) and progression‐free survival than those with a B2MG level <3.2 mg/L (3‐year OS, 50.9% vs. 89.4%, p < 0.001; 3‐year progression‐free survival, 45.3% vs. 79.7%, p < 0.001). Multivariate analysis showed that B2MG, age, performance status, and Ann Arbor stage were independent prognostic factors for OS. We developed a new prognostic model consisting of these four significant factors. We stratified patients into four‐risk groups: low (L, 0 factor), low‐intermediate (LI, 1–2 factors), high‐intermediate (HI, 3 factors), high (H, 4 factors). This new prognostic model showed better risk discrimination compared with the National Comprehensive Cancer Network‐International Prognostic Index (5‐year OS: 100% and 23.4% vs. 100% and 27.1%, in L and H risk groups, respectively). Our study suggested that B2MG level is a significant prognostic factor in patients with DLBCL. A new prognostic index composed of age, performance status, stage, and B2MG could stratify the outcomes of patients with DLBCL effectively and appears to be a valuable risk model for these patients. Copyright © 2016 John Wiley & Sons, Ltd.
Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells
Authors: Zhang Jingzhu , Link Daniel C 
Published: May 2016

ABSTRACT

The targeting specificity of tissue-specific Cre-recombinase transgenes is a key to interpreting phenotypes associated with their use. The Ocn-Cre and Dmp1-Cre transgenes are widely used to target osteoblasts and osteocytes, respectively. Here, we used high-resolution microscopy of bone sections and flow cytometry to carefully define the targeting specificity of these transgenes. These transgenes were crossed with Cxcl12gfp mice to identify Cxcl12-abundant reticular (CAR) cells, which are a perivascular mesenchymal stromal population implicated in hematopoietic stem/progenitor cell maintenance. We show that in addition to osteoblasts, Ocn-Cre targets a majority of CAR cells and arteriolar pericytes. Surprisingly, Dmp1-Cre also targets a subset of CAR cells, in which expression of osteoblast-lineage genes is enriched. Finally, we introduce a new tissue-specific Cre-recombinase, Tagln-Cre, which efficiently targets osteoblasts, a majority of CAR cells, and both venous sinusoidal and arteriolar pericytes. These data show that Ocn-Cre and Dmp1-Cre target broader stromal cell populations than previously appreciated and may aid in the design of future studies. Moreover, these data highlight the heterogeneity of mesenchymal stromal cells in the bone marrow and provide tools to interrogate this heterogeneity. © 2016 American Society for Bone and Mineral Research.
Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?
Authors: Castela Angela , Gomes Pedro , Silvestre Ricardo , Guardão Luísa , Leite Liliana , Chilro Rui , Rodrigues Ilda , Vendeira Pedro , Virag Ronald , Costa Carla 
Published: May 2016

ABSTRACTErectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes‐associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor‐1 alpha (SDF‐1α) expression. Male Wistar rats were divided into groups: age‐matched controls, 8‐weeks streptozotocin‐induced type 1 diabetics, and insulin‐treated 8‐weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF‐1α and testosterone levels were evaluated by ELISA. Penile SDF‐1α protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM‐derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF‐1α levels, which were significantly decreased in animals with diabetes. SDF‐1α protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF‐1α protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes‐induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways. J. Cell. Biochem. 118: 82–91, 2017. © 2016 Wiley Periodicals, Inc.
Perlecan is required for the chondrogenic differentiation of synovial mesenchymal cells through regulation of Sox9 gene expression
Authors: Sadatsuki Ryo , Kaneko Haruka , Kinoshita Mayuko , Futami Ippei , Nonaka Risa , Culley Kirsty L. , Otero Miguel , Hada Shinnosuke , Goldring Mary B. , Yamada Yoshihiko , Kaneko Kazuo , Arikawa‐Hirasawa Eri , Ishijima Muneaki 
Published: May 2016

ABSTRACTWe previously reported that perlecan, a heparan‐sulfate proteoglycan (Hspg2), expressed in the synovium at the cartilage‐synovial junction, is required for osteophyte formation in knee osteoarthritis. To examine the mechanism underlying this process, we examined the role of perlecan in the proliferation and differentiation of synovial mesenchymal cells (SMCs), using a recently established mouse synovial cell culture method. Primary SMCs isolated from Hspg2−/−‐Tg (Hspg2−/−;Col2a1‐Hspg2Tg/−) mice, in which the perlecan‐knockout was rescued from perinatal lethality, lack perlecan. The chondrogenic‐, osteogenic‐, and adipogenic‐potentials were examined in the Hspg2−/−‐Tg SMCs compared to the control SMCs prepared from wild‐type Hspg2+/+‐Tg (Hspg2+/+;Col2a1‐Hspg2Tg/−) littermates. In a culture condition permitting proliferation, both control and Hspg2−/−‐Tg SMCs showed similar rates of proliferation and expression of cell surface markers. However, in micromass cultures, the cartilage matrix production and Sox9 and Col2a1 mRNA levels were significantly reduced in Hspg2−/−‐Tg SMCs, compared with control SMCs. The reduced level of Sox9 mRNA was restored by the supplementation with exogenous perlecan protein. There was no difference in osteogenic differentiation between the control and Hspg2−/−‐Tg SMCs, as measured by the levels of Runx2 and Col1a1 mRNA. The adipogenic induction and PPARγ mRNA levels were significantly reduced in Hspg2−/−‐Tg SMCs compared to control SMCs. The reduction of PPARγ mRNA levels in Hspg2−/−‐Tg SMCs was restored by supplementation of perlecan. Perlecan is required for the chondrogenic and adipogenic differentiation from SMCs via its regulation of the Sox9 and PPARγ gene expression, but not for osteogenic differentiation via Runx2. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:837–846, 2017.
Patterns of major wound complications following multidisciplinary therapy for lower extremity soft tissue sarcoma
Authors: Miller Eric D. , Mo Xiaokui , Andonian Nicole T. , Haglund Karl E. , Martin Douglas D. , Liebner David A. , Chen James L. , Iwenofu Obiajulu H. , Chakravarti Arnab , Scharschmidt Thomas J. , Mayerson Joel L. , Pollock Raphael E. , Xu‐Welliver Meng 
Published: May 2016

Background and ObjectivesThe purpose of this study was to determine the pattern and timing of major wound complications (MWCs) in patients at our institution who received multimodality treatment for lower extremity soft tissue sarcoma (LE‐STS) and to evaluate the impact of MWCs on tumor control and patient outcomes.MethodsThe medical records of 102 LE‐STS patients treated with limb‐sparing surgery and radiation therapy were reviewed. MWCs were defined as secondary operations with anesthesia, seroma/hematoma aspiration, admission for IV antibiotics, or persistent deep packing.ResultsMWCs occurred in 22% of patients, with 45% of events occurring >120 days after resection. On multivariate analysis, preoperative external beam radiation therapy (EBRT) (OR 4.29, 95% CI 1.06–17.40, P = 0.042) and skin graft placement (OR 6.39, 95% CI 1.37–29.84, P = 0.018) were found to be independent predictors of MWCs. MWC occurrence did not predict for chronic toxicity and did not impact tumor control or survival.ConclusionsA considerable proportion of MWCs occur >120 days from surgical resection with preoperative EBRT and skin graft placement independent predictors for MWCs. While an additional source of morbidity, MWC occurrence did not impact tumor control, nor did it predict for chronic toxicity. J. Surg. Oncol. 2016;114:385–391. © 2016 Wiley Periodicals, Inc.
Establishing a danger zone: An anatomic study of the lingual artery in base of tongue surgery
Authors: Cohen David S. , Low Garren M. I. , Melkane Antoine E. , Mutchnick Sean A. , Waxman Jonathan A. , Patel Sagar , Shkoukani Mahdi A. , Lin Ho‐Sheng 
Published: May 2016

Objectives/HypothesisTo contrast the changes in measurement of the hypoglossal/lingual artery neurovascular bundle (HLNVB) to constant surface landmarks in the base of tongue (BOT) during surgically simulated retraction versus resting anatomic position, and to identify a safe zone for BOT robotic surgery to avoid injury to the HLNVB.Study DesignHuman cadaver study.MethodsFive fresh‐frozen head and neck complexes were obtained, and seven HLNVBs were dissected. A microcaliper was used to measure the distance from the HLNVB to constant surface landmarks in resting and surgically simulated positions using a Feyh‐Kastenbauer retractor.ResultsMeasurements from foramen cecum to palatoglossus muscle (P < 0.042) was significantly different when comparing anatomical to surgically simulated positions. Importantly, the location of the lingual artery in reference to the surface landmarks measured was dramatically altered with tongue retraction. With retraction, the branches of the dorsal lingual artery were not encountered posterior to a horizontal line between midway circumvallate papilla (mCVP).ConclusionMeasurements of the HLNVB to surface landmarks in the BOT differs significantly between resting and a surgically simulated tongue position. The dorsal branch of the lingual artery seems more superficial in the BOT than previously described. A safe zone may exist posterior to an imaginary horizontal line between mCVP; however, further studies are needed to confirm this.Level of EvidenceNA Laryngoscope, 127:110–115, 2017
The Impact of Pasture Systems on Soil Microbial Biomass and Community‐level Physiological Profiles
Authors: Cardozo Junior Francisco Marques , Carneiro Romero Francisco Vieira , Rocha Sandra Mara Barbosa , Nunes Luis Alfredo Pinheiro Leal , dos Santos Vilma Maria , de Lima Feitoza Lidiane , de Araújo Ademir Sérgio Ferreira 
Published: May 2016

AbstractThe intensive use of soil for pasture establishment has promoted land degradation. However, well‐managed pasture systems may contribute to the improvement of the soil biological properties. The aim of this study was to evaluate the soil microbial biomass, enzymes activity, and catabolic diversity under six different pastures: Andropogon gayanus, Brachiaria brizantha, Panicum maximum, Cynodon dactilon, agroforestry system, and native vegetation. Soil microbial biomass was estimated by the fumigation‐extraction method, while enzyme activity was determined through substrate use. The catabolic diversity was evaluated by the community‐level physiological profiling method. Microbial biomass C was higher in the soils under the agroforestry system and native vegetation, whereas microbial biomass N was higher in the soil under Brachiaria. Acid phosphatase activity and fluorescein diacetate hydrolysis were higher in agroforestry system and native vegetation, respectively. Dehydrogenase activity was higher in the soil under Brachiaria and Cynodon. Richness and diversity of community‐level physiological profiling were higher in the soils under the agroforestry system and native vegetation. The highest numbers of C sources metabolized by microorganisms were found in the agroforestry system and native vegetation. This study showed that soil microbial community from pasture systems subjected to different management showed different biomass, activity, and catabolic diversity. Copyright © 2016 John Wiley & Sons, Ltd.
Polyaddition of Azide‐Containing Norbornene‐Based Monomer through Strain‐Promoted 1,3‐Dipolar Cycloaddition Reaction
Authors: Zhang Xiaojuan , Zhang Qian , Wu Yuzhen , Feng Chao , Xie Chao , Fan Xiaodong , Li Pengfei 
Published: May 2016

The azide–alkyne “click” reaction has been well known in the past decade, however, another kind of 1,3‐dipolar cycloaddition, the azide–alkene reaction is not fully explored in polymer science to date. This contribution reports, for the first time, the discovery of a polyaddition of norbornene based monomer (NC11N3) containing both strained double bond and azide moieties. The reaction product is characterized by Fourier transform infrared spectroscopy (FTIR), NMR, gel permeation chromatography (GPC), and mass spectrometry (MS), which confirmed the mechanism that is through cycloaddition of azide to strained double bond on norbornene ring to form triazoline linkage. The reaction can proceed at room temperature as indicated by the increase of molecular weight and viscosity during storage. Monomer, dimer, trimer, tetramer, etc., and species with loss of N2 due to lability of triazoline moiety are identified in the mixture of reaction product. As a unique feature, elimination of N2 in the five‐membered ring of triazoline affords a chance to form highly reactive materials, such as with aziridine, which can be a very powerful tool in chemical functionalizations, and find promising applications in reactive polymer resin industries.
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The Art of Compiling Protein Binding Site Ensembles
Authors: Bietz Stefan , Fährrolfes Rainer , Rarey Matthias 
Published: May 2016

AbstractStructure‐based drug design starts with the collection, preparation, and initial analysis of protein structures. With more than 115,000 structures publically available in the Protein Data Bank (PDB), fully automated processes reliably performing these important preprocessing steps are needed. Several tools are available for these tasks, however, most of them do not address the special needs of scientists interested in protein‐ligand interactions. In this paper, we summarize our research activities towards an automated processing pipeline from raw PDB data towards ready‐to‐use protein binding site ensembles. Starting from a single protein structure, the pipeline covers the following phases: Extracting structurally related binding sites from the PDB, aligning disconnected binding site sequences, resolving tautomeric forms and protonation, orienting hydrogens and flippable side‐chains, structurally aligning the multitude of binding sites, and performing a reasonable reduction of ensemble structures. The pipeline, named SIENA, creates protein‐structural ensembles for the analysis of protein flexibility, molecular design efforts like docking or de novo design within seconds. For the first time, we are able to process the whole PDB in order to create a large collection of protein binding site ensembles. SIENA is available as part of the ZBH ProteinsPlus webserver under http://proteinsplus.zbh.uni‐hamburg.de.
Establishing high‐quality reference values for nerve conduction studies: A report from the normative data task force of the American Association Of Neuromuscular & Electrodiagnostic Medicine
Authors: Dillingham Timothy , Chen Shan , Andary Michael , Buschbacher Ralph , Del Toro David , Smith Benn , Zimmermann Kuno , SO Yuen 
Published: May 2016

ABSTRACTIntroduction: There are not uniform standards for nerve conduction testing across the United States. The objective of this study is to present a set of methodologically sound criteria to evaluate the literature for the purpose of identifying high‐quality normative nerve conduction studies (NCS) suitable for widespread use. Methods: The Normative Data Task Force (NDTF) was formed to review published studies on methodological issues related to NCS. A set of criteria was then developed to evaluate the literature. These criteria and their rationale are described. Results: We identified 7 key issues that reflect high quality in NCS. For each issue, specific review criteria were developed. Conclusion: Rigorous criteria enable identification of high‐quality studies dealing with nerve conduction reference values. This represents the first step toward the overarching goal of recommending NCS techniques and reference values for electrodiagnostic medicine. Muscle Nerve 54: 366–370, 2016
Natur häufig Vorbild
Authors: Meinel Lorenz , Seufert Florian , Glaser Jan , Hiltensperger Georg , Holzgrabe Ulrike 
Published: May 2016

AbstractBakterien, Viren und Parasiten lösen Krankheiten aus, die sich mit gängigen Medikamenten oft nicht zufriedenstellend behandeln lassen. Forscher suchen deshalb nach neuen Strukturmotiven für Arzneien.
Verbessert Photokatalyse die Luftqualität?
Authors: George Christian , Herrmann Hartmut , Kleffmann Jörg 
Published: May 2016

AbstractEin europäisches Forschungsprojekt hat untersucht, ob photokatalytische Beschichtungen an Straßen Schadstoffe wie Stickoxide und Kohlenwasserstoffe merklich abbauen und so die Luft reinigen. Messorte waren nicht nur das Labor, sondern auch ein Fahrzeugtunnel und eine Modellstraßenschlucht. Die Photokatalyse funktioniert zwar grundsätzlich, kann aber alleine nicht verhindern, dass die Schadstoffkonzentrationen in der Luft die Grenzwerte überschreiten.
Alge statt Ei?
Authors: Groß Michael 
Published: May 2016

AbstractDie Herstellung von Grippe‐Impfstoffen in Hühnereiern ist zeitaufwendig und umständlich. Auch bei anderen immunologisch relevanten Präparaten ist die Herstellungsmethode der entscheidende Engpass. Für HIV und Hepatitis wurde jetzt die Herstellung mit Algen im Bioreaktor erfolgreich getestet.
Spuren mit Graphitrohr‐AAS bestimmen
Authors: Hupach Sascha 
Published: May 2016

AbstractDie Atomabsorptionsspektroskopie ist eine besonders selektive Methode, um Schwermetalle zu bestimmen. Für den Nachweis im ppm‐Bereich eignet sich die Flammentechnik und zur Spurenanalytik im ppb‐Bereich die Graphitrohrtechnik. Letztere arbeitet mit Inertgas. Eine Gasfluss‐Steuerung beeinflusst die Empfindlichkeit dieser Technik.
Von Silicium zum Perowskit:
Authors: Feldmann Claus , Burgés Dérghon Maia 
Published: May 2016

AbstractDie Wirkungsgrade von Perowskitsolarzellen liegen derzeit bei 20 Prozent und ähneln damit denen von Siliciumsolarzellen, die zwischen 18 und 26 Prozent erreichen. Ihre Basis sind Verbindungen wie Methylammoniumbleitriiodid, das als Siliciumersatz zwar Vorteile hat, aber Blei enthält und weniger stabil ist.
Chemie und Wasser: Das White Paper ist da
Authors: Weinig Hans‐Georg 
Published: May 2016

AbstractFür das von der GDCh 2015 in Leipzig organisierte, sechste Chemical Sciences and Society Symposium (CS3) wurde das Thema Chemistry and Water gewählt und in enger Kooperation mit der Wasserchemischen Gesellschaft – Fachgruppe in der GDCh inhaltlich gestaltet.
On efficient data storage service for IoT
Authors: Karolewicz Konrad , Beben Andrzej , Batalla Jordi Mongay , Mastorakis George , Mavromoustakis Constandinos X. 
Published: May 2016

SummaryThe increasing popularity of Internet of Things (IoT) services causes tremendous growth of the amount of produced data. These data must be effectively stored and retrieved by the IoT service providers. The solution is to access the data through application programming interfaces. The paper focuses on the problem of designing an effective data storage service for IoT, which will be available through the universal application programming interface. We propose and analyze eight variants of the distributed data storage service that differ in the database structure, that is, the hierarchical structure based on Data‐Oriented Network Architecture or the flat structure based on distributed hash table, the type of stored records, and the memory management algorithm that decides about using cache or base memory. The proposed solutions have been evaluated in the comprehensive simulation studies. The obtained results confirmed the effectiveness of the preferred solution with Dynamic Routing Records stored in the cache memory. Copyright © 2016 John Wiley & Sons, Ltd.
Sarcolipin and uncoupling protein 1 play distinct roles in diet‐induced thermogenesis and do not compensate for one another
Authors: Rowland Leslie A. , Maurya Santosh K. , Bal Naresh C. , Kozak Leslie , Periasamy Muthu 
Published: May 2016

ObjectiveIt is well known that uncoupling protein 1 (UCP1) in brown adipose tissue plays an important role in diet‐induced thermogenesis. In this study, whether sarcolipin (SLN), a regulator of sarco/endoplasmic reticulum Ca2+‐ATPase pump in muscle, is also an important player of diet‐induced thermogenesis was investigated, as well as whether loss of SLN could be compensated by increased UCP1 expression and vice versa.MethodsAge‐ and sex‐matched UCP1−/−, SLN−/−, and double knockout for both UCP1 and SLN mice maintained in C57Bl/6J background were challenged to high‐fat diet for 12 weeks and then analyzed for weight gain, alterations in serum metabolites, and changes in thermogenic protein expression.ResultsLoss of either SLN or UCP1 alone was sufficient to cause diet‐induced obesity. No compensatory upregulation of UCP1 in SLN−/− mice or vice versa was found. Paradoxically, loss of both mechanisms failed to exacerbate the obesity phenotype.ConclusionsData suggest that both SLN‐ and UCP1‐based adaptive thermogenic mechanisms were essential for achieving maximal diet‐induced thermogenesis. When both mechanisms were absent, less efficient thermogenic mechanisms were activated to counter energy imbalance.