bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tau excess impairs mitosis and kinesin-5 function, leading to aneuploidy and cell death
Authors: Bougé Anne-Laure , Parmentier Marie-Laure 
Published: January 2016

In neurodegenerative diseases like Alzheimer's disease (AD), cell cycle defects and associated aneuploidy have been described. However, the importance of these defects in the physiopathology of AD and the underlying mechanistic processes are largely unknown in particular with respect to the microtubule-binding protein Tau, which is found in excess in the brain and cerebrospinal fluid of patients. Although it has long been known that Tau is phosphorylated during mitosis to generate a lower affinity for microtubules, there is, to our knowledge, no indication that an excess of this protein could affect mitosis. Here, we studied the effect of an excess of human Tau (hTau) protein on cell mitosis in vivo.
Using the Drosophila developing wing disc epithelium as a model, we show that an excess of hTau induces a mitotic arrest, with the presence of monopolar spindles. This mitotic defect leads to aneuploidy and apoptotic cell death. We studied the mechanism of action of hTau and found that the MT-binding domain of hTau is responsible for these defects. We also demonstrate that hTau effects occur via the inhibition of the function of the kinesin Klp61F, the Drosophila homologue of kinesin-5 (also called Eg5 or KIF11). We finally show that this deleterious effect of hTau is also found in other Drosophila cell types (neuroblasts) and tissues (the developing eye disc) as well as in human Hela cells.
By demonstrating that microtubule-bound Tau inhibits the Eg5/KIF11 kinesin and cell mitosis, our work provides a new framework to consider the role of Tau in neurodegenerative diseases.
Correctors of mutant CFTR enhance subcortical cAMP–PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization
Authors: Abbattiscianni Anna C. , Favia Maria , Mancini Maria T. , Cardone Rosa A. , Guerra Lorenzo , Monterisi Stefania , Castellani Stefano , Laselva Onofrio , Di Sole Francesca , Conese Massimo , Zaccolo Manuela , Casavola Valeria 
Published: January 2016

ABSTRACT
The most common mutation of the cystic fibrosis transmembrane regulator (CFTR) gene, F508del, produces a misfolded protein resulting in its defective trafficking to the cell surface and an impaired chloride secretion. Pharmacological treatments partially rescue F508del CFTR activity either directly by interacting with the mutant protein and/or indirectly by altering the cellular protein homeostasis. Here, we show that the phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate (PIP2) tethers the F508del CFTR to the actin cytoskeleton, stabilizing it on the apical membrane and rescuing the sub-membrane compartmentalization of cAMP and activated PKA. Both the small molecules trimethylangelicin (TMA) and VX-809, which act as ‘correctors’ for F508del CFTR by rescuing F508del-CFTR-dependent chloride secretion, also restore the apical expression of phosphorylated ezrin and actin organization and increase cAMP and activated PKA submembrane compartmentalization in both primary and secondary cystic fibrosis airway cells. Latrunculin B treatment or expression of the inactive ezrin mutant T567A reverse the TMA and VX-809-induced effects highlighting the role of corrector-dependent ezrin activation and actin re-organization in creating the conditions to generate a sub-cortical cAMP pool of adequate amplitude to activate the F508del-CFTR-dependent chloride secretion.
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis
Authors: Atila Metin , Luo Yu 
Published: January 2016

Cationic modulation of the dominantly negative electrostatic structure of phospholipids plays an important role in bacterial response to changes in the environment. In addition to zwitterionic phosphatidylethanolamine, Gram-positive bacteria are also abundant in positively charged lysyl-phosphatidylglycerol. Increased amounts of both types of lipids render Gram-positive bacterial cells more resistant to cationic antibiotic peptides such as defensins. Lysyl and alanyl-phosphatidylglycerol as well as alanyl-cardiolipin have also been studied by mass spectroscopy. Phospholipids modified by other amino acids have been discovered by chemical analysis of the lipid lysate but have yet to be studied by mass spectroscopy. We exploited the high sensitivity of modern mass spectroscopy in searching for substructures in complex mixtures to establish a sensitive and thorough screen for aminoacylated phospholipids. The search for deprotonated aminoacyl anions in lipid extracted fromBacillus subtilisstrain 168 yielded strong evidence as well as relative abundance of aminoacyl-phosphatidylglycerols, which serves as a crude measure of the specificity of aminoacyl-phosphatidylglycerol synthase MprF. No aminoacyl-cardiolipin was found. More importantly, the second most abundant species in this category is D-alanyl-phosphatidylglycerol, suggesting a possible role in the D-alanylation pathway of wall- and lipo-teichoic acids.
Steroid response as prognostic factor and its correlation with molecular assessment of childhood acute lymphoblastic leukemia
Authors: Andriastuti Murti , Gatot Djajadiman , Wirawan Riadi , Setiabudy Rianto , Mansyur Muchtaruddin , Ugrasena I Dewa G. 
Published: January 2016

Background: Survival rate of children with acute lymphoblastic leukemia (ALL) in Indonesia remains low. Risk stratification accuracy is important to improve survival. In developed countries, risk stratification is determined based on gene fusion that is known related to steroid resistency. Steroid response at day-8 correlates with prognosis. The assessment can be applied in centers that cannot perform molecular assessment. This study aims to evaluate whether steroid response correlated to molecular assessment. Methods: A cross-sectional study was performed at Child Health Department, Cipto Mangunkusumo Hospital (January 2013-March 2014), a total of 73 patients were enrolled. Steroid was given for 7 days. Peripheral blast count at day 8 was evaluated, good response if blast count <1000 /µL and poor if ≥1000 /µL. Fusion gene detection was also performed. The data was analysed using Statistical Package for Social Sciences (SPSS) version 20.0.Results: Fusion gene was detected in 45 patients. In 1–10 years age group, 26/32 (81%) subjects had good response, while 75% in <1 year age group and 7/9 (78%) in ≥10 years age group had poor response. 5/7 (71%) subjetcs had leukocyte count >100,000 /µL and 7/8 (88%) with T-cell showed poor response. Age, leukocyte count, and T-cell were statistically correlated with steroid response (p<0.05). E2A-PBX1 fusion gene was the most common 19/45 (42%), followed by TEL-AML1 17/45 (38%), BCR-ABL 5/45 (17%), and MLL-AF4 1/45 (3%). Four of five subjects (80%) with BCR-ABL and one subject with MLL-AF4 had poor steroid response. On the other hand, 12/19 (63%) with E2A-PBX1 and 13/17 (77%) with TEL-AML1 had good response. There was no correlation between steroid response and molecular assessment.Conclusion: Steroid response correlates with age, leukocyte count, and T-cell but not with molecular assessment.
The Physical Activity Support Needs and Strategies Scale: Its Development and Use
Authors: Carbó-Carreté Maria , Guàrdia-Olmos Joan , Giné Climent 
Published: January 2016

Abstract
People with intellectual disability (ID) engage in insufficient physical activity which negatively affects their health. In accordance with the present conception of ID and the supports paradigm, the current study aimed to develop and psychometrically assess an instrument examining the support needs and strategies regarding physical activity by using individuals with ID (n = 529), service providers (n = 522), and family members (n = 462) as information sources. The analysis revealed adequate reliability for the proposed instrument, with α values between .70 and .80, and adequate construct validity for the versions of the scale for the 3 information sources, particularly for service providers. The assessment information can be included in Individualized Support Plans and could be used to design and implement the strategies for facilitating a person's physical activity in their community.
Systematic Review of Health Promotion Programs Focused on Behavioral Changes for People With Intellectual Disability
Authors: Scott Haleigh M. , Havercamp Susan M. 
Published: January 2016

AbstractPeople with intellectual disability (ID) experience high rates of chronic health problems and poor overall health compared to people without disabilities. Recent attention to health risk behaviors such as poor diet, lack of physical activity, and underuse of health care has led to the development of several programs intended to reduce disparities in this population through health promotion programs. A review of the literature was conducted focusing on programs developed to target behavioral changes in the person with ID. Thirteen studies, evaluating 10 different health promotion programs, were found. Programs varied significantly in design, targeted health change, and demonstrated effectiveness. Components of each program are systematically reviewed and recommendations made for future programs based upon the current evidence.
Torus–Connected Cycles: A Simple and Scalable Topology for Interconnection Networks
Authors: Bossard Antoine , Kaneko Keiichi 
Published: January 2016

Abstract
Supercomputers are today made up of hundreds of thousands of nodes. The interconnection network is responsible for connecting all these nodes to each other. Different interconnection networks have been proposed; high performance topologies have been introduced as a replacement for the conventional topologies of recent decades. A high order, a low degree and a small diameter are the usual properties aimed for by such topologies. However, this is not sufficient to lead to actual hardware implementations. Network scalability and topology simplicity are two critical parameters, and they are two of the reasons why modern supercomputers are often based on torus interconnection networks (e.g., Fujitsu K, IBM Sequoia). In this paper we first describe a new topology, torus-connected cycles (TCCs), realizing a combination of a torus and a ring, thus retaining interesting properties of torus networks in addition to those of hierarchical interconnection networks (HINs). Then, we formally establish the diameter of a TCC, and deduce a point-to-point routing algorithm. Next, we propose routing algorithms solving the Hamiltonian cycle problem, and, in a two dimensional TCC, the Hamiltonian path one. Correctness and complexities are formally proved. The proposed algorithms are time-optimal.
The Give and Take Game: Analysis of a Resource Sharing Game
Authors: Mariano Pedro , Correia Luís 
Published: January 2016

Abstract
We analyse Give and Take, a multi-stage resource sharing game to be played between two players. The payoff is dependent on the possession of an indivisible and durable resource, and in each stage players may either do nothing or, depending on their roles, give the resource or take it. Despite these simple rules, we show that this game has interesting complex dynamics. Unique to Give and Take is the existence of multiple Pareto optimal profiles that can also be Nash equilibria, and a built-in punishment action. This game allows us to study cooperation in sharing an indivisible and durable resource. Since there are multiple strategies to cooperate, Give and Take provides a base to investigate coordination under implicit or explicit agreements. We discuss its position in face of other games and real world situations that are better modelled by it. The paper presents an in-depth analysis of the game for the range of admissible parameter values. We show that, when taking is costly for both players, cooperation emerges as players prefer to give the resource.
The Non–Symmetric s–Step Lanczos Algorithm: Derivation of Efficient Recurrences and Synchronization–Reducing Variants of BiCG and QMR
Authors: Feuerriegel Stefan , Bücker H. Martin 
Published: January 2016

Abstract
The Lanczos algorithm is among the most frequently used iterative techniques for computing a few dominant eigenvalues of a large sparse non-symmetric matrix. At the same time, it serves as a building block within biconjugate gradient (BiCG) and quasi-minimal residual (QMR) methods for solving large sparse non-symmetric systems of linear equations. It is well known that, when implemented on distributed-memory computers with a huge number of processes, the synchronization time spent on computing dot products increasingly limits the parallel scalability. Therefore, we propose synchronization-reducing variants of the Lanczos, as well as BiCG and QMR methods, in an attempt to mitigate these negative performance effects. These so-called s-step algorithms are based on grouping dot products for joint execution and replacing time-consuming matrix operations by efficient vector recurrences. The purpose of this paper is to provide a rigorous derivation of the recurrences for the s-step Lanczos algorithm, introduce s-step BiCG and QMR variants, and compare the parallel performance of these new s-step versions with previous algorithms.
Ergodicity and Perturbation Bounds for Inhomogeneous Birth and Death Processes with Additional Transitions from and to the Origin
Authors: Zeifman Alexander , Korotysheva Anna , Satin Yacov , Korolev Victor , Shorgin Sergey , Razumchik Rostislav 
Published: January 2016

Abstract
Service life of many real-life systems cannot be considered infinite, and thus the systems will be eventually stopped or will break down. Some of them may be re-launched after possible maintenance under likely new initial conditions. In such systems, which are often modelled by birth and death processes, the assumption of stationarity may be too strong and performance characteristics obtained under this assumption may not make much sense. In such circumstances, time-dependent analysis is more meaningful. In this paper, transient analysis of one class of Markov processes defined on non-negative integers, specifically, inhomogeneous birth and death processes allowing special transitions from and to the origin, is carried out. Whenever the process is at the origin, transition can occur to any state, not necessarily a neighbouring one. Being in any other state, besides ordinary transitions to neighbouring states, a transition to the origin can occur. All possible transition intensities are assumed to be non-random functions of time and may depend (except for transition to the origin) on the process state. To the best of our knowledge, first ergodicity and perturbation bounds for this class of processes are obtained. Extensive numerical results are also provided.
Nonlinear System Identification with a Real–Coded Genetic Algorithm (RCGA)
Authors: Cherif Imen , Fnaiech Farhat 
Published: January 2016

Abstract
This paper is devoted to the blind identification problem of a special class of nonlinear systems, namely, Volterra models, using a real-coded genetic algorithm (RCGA). The model input is assumed to be a stationary Gaussian sequence or an independent identically distributed (i.i.d.) process. The order of the Volterra series is assumed to be known. The fitness function is defined as the difference between the calculated cumulant values and analytical equations in which the kernels and the input variances are considered. Simulation results and a comparative study for the proposed method and some existing techniques are given. They clearly show that the RCGA identification method performs better in terms of precision, time of convergence and simplicity of programming.
Application of Cubic Box Spline Wavelets in the Analysis of Signal Singularities
Authors: Rakowski Waldemar 
Published: January 2016

Abstract
In the subject literature, wavelets such as the Mexican hat (the second derivative of a Gaussian) or the quadratic box spline are commonly used for the task of singularity detection. The disadvantage of the Mexican hat, however, is its unlimited support; the disadvantage of the quadratic box spline is a phase shift introduced by the wavelet, making it difficult to locate singular points. The paper deals with the construction and properties of wavelets in the form of cubic box splines which have compact and short support and which do not introduce a phase shift. The digital filters associated with cubic box wavelets that are applied in implementing the discrete dyadic wavelet transform are defined. The filters and the algorithme à trous of the discrete dyadic wavelet transform are used in detecting signal singularities and in calculating the measures of signal singularities in the form of a Lipschitz exponent. The article presents examples illustrating the use of cubic box spline wavelets in the analysis of signal singularities.
Neural Networks as a Tool for Georadar Data Processing
Authors: Szymczyk Piotr , Tomecka-Suchoń Sylwia , Szymczyk Magdalena 
Published: January 2016

Abstract
In this article a new neural network based method for automatic classification of ground penetrating radar (GPR) traces is proposed. The presented approach is based on a new representation of GPR signals by polynomials approximation. The coefficients of the polynomial (the feature vector) are neural network inputs for automatic classification of a special kind of geologic structure—a sinkhole. The analysis and results show that the classifier can effectively distinguish sinkholes from other geologic structures.
Existence and asymptotic behavior of ground state solutions of semilinear elliptic system
Authors: Mâagli Habib , Ben Othman Sonia , Dridi Safa 
Published: January 2016

AbstractIn this article, we take up the existence and the asymptotic behavior of entire bounded positive solutions to the following semilinear elliptic system:-Δu=a1a_{1}(x)uαu^{\alpha}vrv^{r},x∈\inℝn\mathbb{R}^{n}(n≥\geq3), -Δv=a2a_{2}(x)vβv^{\beta}usu^{s},x∈\inℝn\mathbb{R}^{n},u,v¿ 0 inℝn\mathbb{R}^{n},lim|x|→+∞\lim_{|x|\rightarrow+\infty}u(x) =lim|x|→+∞\lim_{|x|\rightarrow+\infty}v(x)=0,whereα,β<1{\alpha,\beta<1},r,s∈ℝ{r,s\in\mathbb{R}}such thatν:=(1-α)⁢(1-β)-r⁢s>0{\nu:=(1-\alpha)(1-\beta)-rs>0}, and the functionsa1a_{1},a2a_{2}are nonnegative in𝒞locγ⁢(ℝn){\mathcal{C}^{\gamma}_{\mathrm{loc}}(\mathbb{R}^{n})}(0¡γ¡1) and satisfy some appropriate assumptions related to Karamata regular variation theory.
Cross-species PCR and field studies on Paulownia elongata: A potential bioenergy crop
Authors: Basu Chhandak , Joshee Nirmal , Gezalian Tigran , Vaidya Brajesh Nanda , Satidkit Asada , Hemmati Homa , Perry Zachary David 
Published: January 2016

AbstractPaulownia elongata is a short-rotation fast growing tree and is known for high biomass accumulation and carbon sequestration potential. Optimization of protocols for nucleic acid extraction, PCR, RT-PCR, and other molecular biology techniques are required for better understanding of cellulose synthesis and to assess the potential of Paulownia as a biofuel tree. The main objective of this work was to study a putative cellulose synthase amplicon expression under various environmental conditions and evaluate the potentials of Paulownia as a biofuel tree. Using cross-species PCR an amplicon representative of a putative cellulose synthase gene from Paulownia was identified. This 177-bp long DNA sequence was 46% similar with cellulose synthase genes from Arabidopsis as expected. Gene specific primers for this particular Paulownia cellulose synthase gene were designed and reverse transcription PCR was performed to confirm its transcription. We report an inexpensive cDNA dot-blot method to study expression of this gene under various environmental conditions. We observed that cold and, to a lesser extent, heat stress downregulated its expression. This information will help to understand cellulose deposition in plant cell wall under stressful conditions. To the best of our knowledge this is the first characterization of a cDNA sequence from Paulownia elongata.
Alkali treatment of fungal pretreated wheat straw for bioethanol production
Authors: García-Torreiro María , Pallín Miguel Álvarez , López-Abelairas María , Lu-Chau Thelmo A. , Lema Juan M. 
Published: January 2016

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.
Life in the cold: links between mammalian hibernation and longevity
Authors: Wu Cheng-Wei , Storey Kenneth B. 
Published: January 2016

AbstractThe biological process of aging is the primary determinant of lifespan, but the factors that influence the rate of aging are not yet clearly understood and remain a challenging question. Mammals are characterized by >100-fold differences in maximal lifespan, influenced by relative variances in body mass and metabolic rate. Recent discoveries have identified long-lived mammalian species that deviate from the expected longevity quotient. A commonality among many long-lived species is the capacity to undergo metabolic rate depression, effectively re-programming normal metabolism in response to extreme environmental stress and enter states of torpor or hibernation. This stress tolerant phenotype often involves a reduction in overall metabolic rate to just 1–5% of the normal basal rate as well as activation of cytoprotective responses. At the cellular level, major energy savings are achieved via coordinated suppression of many ATP-expensive cell functions; e.g. global rates of protein synthesis are strongly reduced via inhibition of the insulin signaling axis. At the same time, various studies have shown activation of stress survival signaling during hibernation including up-regulation of protein chaperones, increased antioxidant defenses, and transcriptional activation of pro-survival signaling such as the FOXO and p53 pathways. Many similarities and parallels exist between hibernation phenotypes and different long-lived models, e.g. signal transduction pathways found to be commonly regulated during hibernation are also known to induce lifespan extension in animals such asDrosophila melanogasterandCaenorhabditis elegans. In this review, we highlight some of the molecular mechanisms that promote longevity in classic aging modelsC. elegans, Drosophila, and mice, while providing a comparative analysis to how they are regulated during mammalian hibernation.