bluuebunny/crossref_metadata_2025_split huggingface dataset:
CHO stable pool fed‐batch process development of SARS‐CoV‐2 spike protein production: Impact of aeration conditions and feeding strategies
Authors: Reyes Sebastian‐Juan , Pham Phuong Lan , Durocher Yves , Henry Olivier 
Published: September 2024

AbstractTechnology scale‐up and transfer are a fundamental and critical part of process development in biomanufacturing. Important bioreactor hydrodynamic characteristics such as working volume, overhead gas flow rate, volumetric power input (P/V), impeller type, agitation regimen, sparging aeration strategy, sparger type, and kLa must be selected based on key performance indicators (KPI) to ensure a smooth and seamless process scale‐up and transfer. Finding suitable operational setpoints and developing an efficient feeding regimen to ensure process efficacy and consistency are instrumental. In this investigation, process development of a cumate inducible Chinese hamster ovary (CHO) stable pool expressing trimeric SARS‐CoV‐2 spike protein in 1.8 L benchtop stirred‐tank bioreactors is detailed. Various dissolved oxygen levels and aeration air caps were studied to determine their impact on cell growth and metabolism, culture longevity, and endpoint product titers. Once hydrodynamic conditions were tuned to an optimal zone, various feeding strategies were explored to increase culture performance. Dynamic feedings such as feeding based on current culture volume, viable cell density (VCD), oxygen uptake rate (OUR), and bio‐capacitance signals were tested and compared to standard bolus addition. Increases in integral of viable cell concentration (IVCC) (1.25‐fold) and protein yield (2.52‐fold), as well as greater culture longevity (extension of 5 days) were observed in dynamic feeding strategies when compared to periodic bolus feeding. Our study emphasizes the benefits of designing feeding strategies around metabolically relevant signals such as OUR and bio‐capacitance signals.
Cross‐sectional online survey on the perspectives of more than 1100 Austrian therapists on integrating nature in psychotherapy
Authors: Aigner Sandra Elisabeth , Dale Rachel , Pfeifer Eric , Probst Thomas , Winter Stefanie , Herburger Bernhard , Pieh Christoph , Humer Elke 
Published: September 2024

AbstractBackgroundIntegrating natural environments into therapeutic practices is increasingly recognised for its positive effects on mental health recovery. However, there is limited research on how nature is utilised in psychotherapeutic treatments. This study aimed to fill this gap by providing initial data on the frequency and application of nature‐integrating psychotherapy. Additionally, it explores psychotherapists' attitudes towards the potential and limitations of integrating nature into psychotherapy under practical conditions.MethodsA total of N = 1190 Austrian psychotherapists (mean age: 53.75 ± 10.84 years; 74.9% female; 19.9% psychodynamic, 47.9% humanistic, 23.0% systemic, 9.2% behavioural) participated in a cross‐sectional online survey assessing personality traits (Big Five Inventory), connectedness to nature (NCI), professional experiences in integrating aspects of nature in psychotherapy and estimated potentials and risks of nature‐integrating interventions.ResultsThe majority of participating psychotherapists reported experience with conducting psychotherapy in nature (65.9%) and with integrating nature materials into their treatments (79.1%). Differences in some personality traits among psychotherapeutic orientations were observed, whereas the NCI did not differ. Overall, systemic therapists had the most positive attitude towards integrating nature into psychotherapy, whereas the opposite was observed for psychodynamic psychotherapists. Several aspects were found to also be affected by an interaction between orientation and gender.ConclusionsResults indicate that nature‐integrating psychotherapy is both common and valued among Austrian psychotherapists, with differences observed across therapeutic orientations and genders. These differences highlight the need for further research to better understand the factors influencing psychotherapists' approaches to nature integration in clinical practice.
Taking a tiny step back: The impact of planning on a bumpy goal pursuit
Authors: Bayuk Julia , Basoglu K. Asli 
Published: September 2024

AbstractAcross varied domains, prior research has examined the impact of setbacks and goal‐inconsistent behaviors on goal pursuit. A large setback, whether a result of an external hurdle or an internally‐driven goal‐inconsistent behavior, may suggest that the goal is not really feasible and goal disengagement is ideal. Yet, a minor setback or a goal‐deviation may just be a bump in the road that can, and often should, be overcome, especially for important, long‐term goals. So, not all setbacks signal that failure is inevitable and goal pursuit should be put on hold. Forming a goal‐directed plan shifts individuals from a deliberative to an implemental mindset and is commonly recommended by both academics and practitioners to help individuals pursue important goals and disengage from unachievable goals. But are plans beneficial when small, goal‐related setbacks occur? Specifically, this article examines the impact of minor bumps in the road (experiencing small setbacks, engaging in minor goal‐inconsistent behaviors) on continued goal pursuit. In three studies, field and lab, and across two different domains (eating healthy and saving money), we (1) show that small, seemingly acceptable setbacks can negatively influence goal pursuit when one has formed a plan, and (2) empirically demonstrate that when small setbacks occur, having a plan may increase feeling of action crisis and ultimately decrease interest in continued goal pursuit.
Rapid Screening of Active Components with Thrombin Inhibitory Activity from Peach Kernel Based on Affinity Ultrafiltration Coupled with UPLC‐Q‐TOF‐MS
Authors: Huang Shiyi , Zhang Jiajia , He Xiangchang , Huang Chencun , He Weihe , Zhao Hongqing , Dai Jie , Xu Guangming 
Published: September 2024

AbstractIntroduction: Peach kernel (PK), one of the medicinal and edible plants, has been widely used in the treatment of clinical thrombotic diseases and exhibited great therapeutic effects. However, the effective substances and targets are still obscure. Methods: A method consisting of affinity ultrafiltration (AUF) coupled with ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectroscopy (UPLC−Q‐TOF‐MS) was established to rapidly screen active components that inhibit thrombin, a key mediator in coagulation cascade. The binding energy and sites were analyzed by molecular docking to evaluate the binding capacity of thrombin‐ligand complexes, and the thrombin inhibitory activity of screened ligands was further validated via in vitro and in vivo tests. Results: two potential thrombin ligands (L‐arginine and cytidine) were screened by AUF‐HPLC and identified by UPLC−Q‐TOF‐MS. The ligands with anti‐thrombin structure exhibited great affinity with thrombin. The anticoagulant bioactivity of ligands was validated by a significant reduction in thrombosis in zebrafish tails and the potential mechanism could be related to direct inhibition of thrombin. Conclusion: The systematic screening of anti‐thrombin active components in PK based on AUF‐UPLC−Q‐TOF‐MS is a feasible and effective method, providing valuable information for the future development of direct thrombin inhibitors.
In Vitro Assessment of Immunobiological Effectivity of Synthetic Non‐Ionic Glycolipids
Authors: Paulovičová Ema , Paulovičová Lucia , Poláková Monika 
Published: September 2024

AbstractImmunobiological activity of selected decyl and (thio)dodecyl hexopyranosides based on d‐glucose, d‐galactose, N‐acetyl d‐glucosamine and d‐mannose and their effect on leukemia cell lines L1210 and HL‐60 and Candida‘s biofilm were studied. Alkyl d‐glucosides and d‐galactosides showed mainly similar antiproliferative properties on leukemia cell lines, while N‐acetyl d‐glucosaminides revealed diverse properties with lower efficacy. Also, the cytokine response of RAW 264.7 macrophages was significantly influenced by the type of sugar moiety and (thio)alkyl chain length. Contrary to the proliferation results, d‐glucosides and d‐galactosides did not reveal so evident similarities in induction of cytokines. The C. albicans biofilm treatment with the (thio)alkyl glycosides resulted in a significant reduction of Candida cell proliferation resembling the structure and concentration differences of glycosides. The activity of tested derivatives (GalOC12 > GlcOC12 ≈ ManOC12 > GlcNAcOC12) against the C. albicans azole‐sensitive clinical strain biofilm differ from the efficacy against C. albicans multiazole‐resistant clinical strain biofilm (GlcOC12 > ManOC12 ≈ GalOC12 > GlcNAcOC12). The obtained data clearly demonstrated that the structure of saccharide unit caused different bioimmunological behaviour of such glycosides regardless of the same aglycone length.
Synthesis, Characterization and Antibacterial Activity Evaluation of CuO NPs and B‐CuO NPs Obtained from Livistona Chinensis Leaf Extract
Authors: Veg Ekhlakh , Raza Azam , Rai Smita , Sharma Swati , Pandey Anushka , Imran Ahmad Mohammad , Jabeen Sabeeha , Joshi Seema , Khan Tahmeena 
Published: September 2024

AbstractNanoparticles (NPs) exhibit fascinating size‐dependent chemical and physical characteristics that make them useful for a variety of applications. The present paper reports the green synthesis of CuO NPs and B‐doped CuO NPs (B‐CuO NPs) from Livistona chinensis leaf extract. Not much work has been reported on the use of the plant extract for the fabrication of NPs, particularly those of Cu and its doped counterparts. Various spectroscopic techniques were used to characterize the synthesized NPs. In the FT‐IR spectra, peaks obtained at 504 cm−1 to 600 cm−1 were due to Cu−O vibrations. The energy dispersive X‐ray analysis (EDX) spectra confirmed the CuO NPs’ composition and B's presence inside the NPs. The peak pattern in the X‐ray diffraction (XRD) spectrum confirmed the crystalline and monoclinic phases of the NPs. The average crystalline size of CuO NPs and B‐CuO NPs was 19.56 nm and 17.30 nm respectively. The CuO and B‐CuO NPs were tested against three Gram‐positive bacterial strains namely Bacillus subtilis, Micrococcus luteus, and Staphylococcus aureus and three Gram‐negative Escherichia coli, Salmonella abony, and Pseudomonas aeruginosa through Agar well diffusion method and it was found that CuO NPs showed higher activity than B‐CuO NPs. Gentamicin was used as the positive control. The antibacterial activity may be due to the cell wall disruption by induction of innate and adaptive host immune response, generating toxic reactive oxygen species (ROS), and stimulating intracellular effects.
Novel Sulfonylamido Benzoxazole Derivatives: Synthesis, Characterisation, Molecular Docking, DFT, and Antimicrobial Activity Investigations
Authors: Faydali Nagihan , Erol Meryem , Temiz Arpaci Ozlem , Kuyucuklu Gulcan , Semih Salan Alparslan 
Published: September 2024

AbstractIn this work, numerous novel 2‐(p‐ethyl/fluorophenyl)‐5‐[(p‐substitutedphenyl)sulfonylamido]benzoxazole derivatives were designed, synthesized, and structurally characterized using 1H‐NMR, 13C‐NMR, mass spectroscopy, and elemental analysis approaches. The antimicrobial activity against several Gram (+) bacteria, Gram (−) bacteria, and fungal species was determined using the in vitro microdilution technique. A molecular docking analysis was performed on all produced compounds utilizing the S. aureus gyrase complex with ciprofloxacin and DNA. Two of the most effective compounds against S. aureus, N4 and N9, have binding energies of −8.7 kcal/mol and −8.6 kcal/mol, respectively, and their interactions have been demonstrated in 2D and 3D. Furthermore, utilizing the 6–311G(d,p) base set and DFT/B3LYP theory, MEP analysis, geometric optimization, and molecular reactivity analysis (HOMO‐LUMO) of N4 and N9 were performed, and the results were presented. All compounds’ theoretical ADMET profiles were computed as well. With all of this knowledge, this study could be a pioneer in the development of novel anti‐S. aureus compounds.
Glycerol as a Feedstock for Chemical Synthesis
Authors: Husna Maratul , Tabak Yasemin , Yıldız Meltem 
Published: September 2024

AbstractGlycerol, defined simply as a colorless, sweet syrupy liquid extracted from fatty substances through saponification, is an alcohol with three hydroxyl (OH–) groups in its structure. Glycerol has many uses in the consumer market. It is used primarily in personal care products, as an adhesive and sealing agent and many applications. Glycerol, whose name is propane‐1,2,3‐triol, standardized by the International Union of Pure and Applied Chemistry (IUPAC), CHO open formula CH2OH–CHOH–CH2OH. It can be said that glycerol, a by‐product of biodiesel, is produced in very high quantities. Retention of the produced glycerol will lead to cost increases and environmental problems that may directly affect the development of the biodiesel market. Due to the supply of glycerol to the market in large quantities, glycerol prices have hit the bottom, and therefore, the income and profitability of biodiesel production factories from the sale of glycerol have decreased. This situation clearly shows that the excess of glycerol now poses an obstacle to developing the biodiesel market. This article aims to list the valuable chemicals into which glycerol, produced in large quantities as a biodiesel by‐product, can be converted under a single heading and to detail the studies carried out on this subject.
TYRO3 and EPHA2 Expression Are Dysregulated in Breast Cancer
Authors: de Aguiar Ananda Cristina Fernandes , Ferreira Nancy Cristina Ferraz de Lucena , Borba Maria Amelia Carlos Souto Maior , Filho Darley de Lima Ferreira , Leitão Glauber Moreira , Mattos Luiz Alberto , Filho José Luiz de Lima , Martins Danyelly Bruneska Gondim 
Published: September 2024

ABSTRACTReceptor tyrosine kinases (RTKs) are involved in cell growth, motility, and differentiation. Deregulation of RTKs signaling is associated with tumor development and therapy resistance. Potential RTKs like TAM (TYRO3, AXL, MERTK), RON, EPH, and MET have been evaluated in many cancers like lung, prostate, and colorectal, but little is known in breast tumors. In this study, 51 luminal breast cancer tissue and 8 triple negative breast cancer (TNBC) subtypes were evaluated by qPCR for the expression of TAM, RON, EPHA2, and MET genes. Statistical analysis was performed to determine the correlation to clinical data. TYRO3 is related to tumor subtype and stage, patient's age, smoking habits, and obesity. MET expression is correlated to EPHA2 and TAM gene expression. EPHA2 expression is also related to aging and smoking habits. The expression levels of the TAM and EPHA2 genes seem to play an important role in breast cancer, being also influenced by the patient's lifestyle.
The Structural Diversity of Encapsulin Protein Shells
Authors: Giessen Tobias W. 
Published: September 2024

AbstractSubcellular compartmentalization is a universal feature of all cells. Spatially distinct compartments, be they lipid‐ or protein‐based, enable cells to optimize local reaction environments, store nutrients, and sequester toxic processes. Prokaryotes generally lack intracellular membrane systems and usually rely on protein‐based compartments and organelles to regulate and optimize their metabolism. Encapsulins are one of the most diverse and widespread classes of prokaryotic protein compartments. They self‐assemble into icosahedral protein shells and are able to specifically internalize dedicated cargo enzymes. This review discusses the structural diversity of encapsulin protein shells, focusing on shell assembly, symmetry, and dynamics. The properties and functions of pores found within encapsulin shells will also be discussed. In addition, fusion and insertion domains embedded within encapsulin shell protomers will be highlighted. Finally, future research directions for basic encapsulin biology, with a focus on the structural understand of encapsulins, are briefly outlined.
HydroDynamic contrast Recanalization (HDR): Description of a new crossing technique for coronary chronic total occlusions
Authors: Carlino Mauro , Nascimbene Angelo , Brilakis Emmanouil S. , Yarrabothula Akshitha , Colombo Antonio , Nakamura Sunao , Azzalini Lorenzo , Hanif Bashir , Iqbal M. Bilal , Arain Salman A. 
Published: September 2024

AbstractBackgroundIntraplaque delivery of contrast has been utilized during percutaneous coronary interventions (PCI) of chronic total occlusions (CTO) to delineate anatomy and to facilitate wire crossing. Its utility as a tool to accomplish primary crossing of CTOs has not been described or validated.AimsWe describe a new technique leveraging the diagnostic and therapeutic roles of intraplaque contrast injection to accomplish primary crossing of CTOs: HydroDynamic contrast Recanalization (HDR).MethodsHDR is an antegrade crossing method for coronary CTOs based on the synergistic use of contrast microinjections and polymer jacketed wires. We present a retrospective, first‐in‐man, case series utilizing HDR for CTO PCI in patients with favorable CTO anatomy (visible proximal segment and identifiable distal target). The primary outcome was procedural success. The secondary outcome was any procedural complications.ResultsA total of 43 patients with 45 CTOs underwent CTO PCI with HDR. Mean patient age was 64.3 ± 11 years. The mean Japanese CTO and PROGRESS CTO scores were 2.3 ± 0.7 and 1.8 ± 0.7, respectively. CTO complexity was high, with an ambiguous or blunt cap in 34 occlusions (76%); lesion length ≥ 20 mm in 27 occlusions (60%); and moderate/heavy calcification in 36 occlusions (80%). Procedural success using HDR was 100%. There were no complications.ConclusionsThis study shows the utility of HDR in CTO PCI. HDR appears to be a safe and promising new contrast‐based primary crossing technique in selected patients. This strategy warrants further evaluation in larger prospective studies.
Asymmetric Alkylative Difunctionalization of Carbon–Carbon Double Bonds by Aliphatic C─H Bond Activation
Authors: Yan Zilong , Wang Shang , Mao Xinyu , Xing Dong 
Published: September 2024

AbstractThis paper highlights the latest advancements in asymmetric alkylative difunctionalization of carbon–carbon double bonds by utilizing aliphatic C─H substrates as alkyl radical precursors. By integrating different hydrogen atom transfer (HAT) processes with various transition metal catalytic systems, various alkane substrates can be efficiently converted into corresponding alkyl radicals, which then participate in radical additions to carbon–carbon double bonds followed by stereoselective functionalizations with a third component. These strategies leverage inexpensive, abundant, and readily available alkane feedstocks to rapidly construct complex chiral molecules, demonstrating high step and atom economy. This paper focuses on recent progress and applications in this area, provides a comprehensive perspective on current developments, and suggests future directions in alkane‐involved asymmetric transformations.
Scientific challenges faced by Mn‐based layered oxide cathodes with anionic redox for sodium‐ion batteries
Authors: Zheng Chao , He Shengnan , Gan Jiantuo , Wu Zhijun , She Liaona , Gao Yong , Yang YaXiong , Lou Jiatao , Ju Zhijin , Pan Hongge 
Published: September 2024

AbstractIn the realm of sodium‐ion batteries (SIBs), Mn‐based layered oxide cathodes have garnered considerable attention owing to their anionic redox reactions (ARRs). Compared to other types of popular sodium‐ion cathodes, Mn‐based layered oxide cathodes with ARRs exhibit outstanding specific capacity and energy density, making them promising for SIB applications. However, these cathodes still face some scientific challenges that need to be addressed. This review systematically summarizes the composition, structure, oxygen‐redox mechanism, and performance of various types of Mn‐based cathodes with ARRs, as well as the main scientific challenges they face, including sluggish ion diffusion, cationic migration, O2 release, and element dissolution. Currently, to resolve these challenges, efforts mainly focus on six aspects: synthesis methods, structural design, doped modification, electrolyte design, and surface engineering. Finally, this review provides new insights for future direction, encompassing both fundamental research, such as novel cathode types, interface optimization, and interdisciplinary research, and considerations from an industrialization perspective, including scalability, stability, and safety.
Agronomic performance of soybean with varied planting dates, row configurations, and seeding rates on two different soil textures
Authors: Kelly F. Read , Bond Jason A. , Bryant Corey J. , Irby J. Trenton , Cook Don R. , Krutz L. Jason 
Published: September 2024

AbstractPlanting date, row configuration, and seeding rate are three critical factors in obtaining maximum soybean [Glycine max (L.) Merr.] grain yield and can vary based on soil texture. Therefore, two studies were conducted at the Delta Research and Extension Center in Stoneville, MS. The first study was conducted from 2019 to 2021 and sought to determine the effects of planting date (optimal and delayed 21 days), and row configuration (single‐, twin‐, and triple‐row) on soybean growth, development, and grain yield. The second study was conducted in 2021 with three site‐years to determine the effects of seeding rate (130,000, 180,000, and 220,000 seeds acre−1) in a triple‐row configuration on soybean grain yield compared to a single‐row configuration at 130,000 seeds acre−1 on two soil textures (silt loam and clay). Both studies were repeated on silt loam and clay soil textures in every site‐year. In the first study, the optimal planting date increased soybean grain yield regardless of soil texture. On both soil textures, twin‐ and single‐row configuration yields were equivalent, but triple‐row configuration reduced soybean grain yield up to 9%. Similarly, triple‐row configuration reduced soybean density and height at R3 and R8 growth stages. In the second study, increasing triple‐row configuration soybean seeding rate by at least 38% provided similar soybean grain yields to a single‐row configuration at 130,000 seeds acre−1. These data indicate that triple‐row soybean planting configurations do have some benefits, but that future research should focus on equipment limitations experienced in the current research.