bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feathers, folklore, and eco-literacy: Stories ascribe cultural keystone status to avian scavengers in South Asian cities
Authors: Gupta Urvi , Kumar Nishant 
Published: October 2024

ABSTRACT
We examined the cultural significance of commensal avian scavenger species—vultures, kites, and crows—and their exploitation of anthropogenic resources and sentiments within Delhi’s urban landscapes. For this, we investigated the intrinsic values attributed to these birds by people, which are indicative of complex, rapidly urbanizing social-ecological systems. Semi-structured interviews revealed folk perceptions intertwined with socio-cultural narratives and traditions, shaped by observations of avian morphology, ecology, and behavior. Birds’ nesting habits, habitats, home ranges, and foraging behaviors affected people’s perceptions, while ecosystem services inspired zoomorphism and anthropomorphism via vernacular-nomenclature (e.g., chidiya collectively for songbirds, giddha for scavenging raptors). Culturally rooted perceptions, which informed ritual feeding practices and shaped prevalent attitudes toward commensal species, fostered mutual tolerance, and brought people into closer contact with urban biodiversity. Such physical and cultural proximity is a defining characteristic distinguishing tropical urban ecosystems from their Western counterparts. We also uncovered the web of social-technological influences on animal-related folk stories. The urbanization of perceptions in vulture extinction zones revealed shifts in social–ecological relationships with wildlife. It adds cultural dimensions to the currently appreciated keystone status of vultures, vital for their erstwhile coexistence at extremely high densities in South Asia. Urban transformations, technological advancements, and media exposure potentially reshaped human–animal interface, with media misinformation affecting personalized ecologies. Conflicts and health concerns arose from media narratives on garbage-consuming animals. Our findings offer insights to prevent severing of people and nature connections due to urbanization (e.g., technological applications can integrate scientific knowledge with biocultural narratives and folklore), promoting a new-age eco-literacy.
AUXIN RESPONSE FACTOR 2 mediates repression of strawberry receptacle ripening via auxin-ABA interplay
Authors: Li Bai-Jun , Shi Yan-Na , Xiao Yan-Ning , Jia Hao-Ran , Yang Xiao-Fang , Dai Zheng-Rong , Sun Yun-Fan , Shou Jia-Han , Jiang Gui-Hua , Grierson Donald , Chen Kun-Song 
Published: October 2024

Abstract
Cultivated strawberry (Fragaria × ananassa) is a popular, economically important fruit. The ripening of the receptacle (pseudocarp), the main edible part, depends on endogenously produced abscisic acid (ABA) and is suppressed by the high level of auxin produced from achenes (true fruit) during early development. However, the mechanism whereby auxin regulates receptacle ripening through inhibiting ABA biosynthesis remains unclear. Here, we identified AUXIN RESPONSE FACTOR 2 (FaARF2), which showed decreased expression with reduced auxin content in the receptacle, leading to increased ABA levels and accelerated ripening. Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays demonstrated that FaARF2 could bind to the AuxRE element in the promoter of 9-CIS-EPOXYCAROT-ENOID DIOXYGENASE 1 (FaNCED1), a key ABA biosynthetic gene, to suppress its transcriptional activity. Transiently overexpressing FaARF2 in the receptacles decreased FaNCED1 expression and ABA levels, resulting in inhibition of receptacle ripening and of development of quality attributes, such as pigmentation, aroma, and sweetness. This inhibition caused by overexpressing FaARF2 was partially recovered by the injection of exogenous ABA; conversely, transient silencing of FaARF2 using RNA interference produced the opposite results. The negative targeting of FaNCED1 by FaARF2 is a key link between auxin-ABA interactions and regulation of strawberry ripening.
Noninvasive brain stimulation beyond the motor cortex: a systematic review and meta-analysis exploring effects on quantitative sensory testing in clinical pain
Authors: Flood Andrew , Cavaleri Rocco , Chang We-Ju , Kutch Jason , Toufexis Constantino , Summers Simon J 
Published: October 2024

Abstract

Background
Noninvasive brain stimulation (NIBS) has been investigated increasingly as a means of treating pain. The effectiveness of NIBS in the treatment of pain has traditionally focused on protocols targeting the primary motor cortex (M1). However, over time, the effectiveness of M1 NIBS has been attributed to effects on interconnected cortical and subcortical sites rather than on M1 itself. Although previous reviews have demonstrated the effectiveness of non-M1 NIBS in improving subjective reports of pain intensity, the neurophysiological mechanisms underlying these effects remain incompletely understood. As chronic pain is associated with pain hypersensitivity and impaired endogenous descending pain modulation, it is plausible that non-M1 NIBS promotes analgesic effects by influencing these processes.

Objective
The aim of this systematic review and meta-analysis was therefore to evaluate the effect of NIBS over non-M1 sites on quantitative sensory testing measures in clinical pain populations.

Methods
A systematic search of electronic databases was conducted from inception to January 2024. Included articles (13 trials, n = 565 participants) were appraised with the Physiotherapy Evidence Database (PEDro) scale and Grading of Recommendations, Assessment, Development, and Evaluation (GRADE), and a random-effects model was used to meta-analyze outcomes where possible.

Results
A small number of studies found that NIBS applied to the dorsolateral prefrontal cortex might improve pain modulation in patients with fibromyalgia and that stimulation of the posterior superior insula and prefrontal cortex could improve pain sensitivity in chronic neuropathic and osteoarthritic pain, respectively. However, findings varied among studies, and there remains a paucity of primary research.

Conclusion
This review indicates that current literature does not provide clear evidence that NIBS over non-M1 sites influences pain processing.

Study registration
PROSPERO (CRD42020201076)
Descriptive norms can “backfire” in hyper-polarized contexts
Authors: Rand David G , Yoeli Erez 
Published: October 2024

Abstract
Descriptive social norms interventions, where a behavior is promoted by learning that others engage in that behavior, are a cornerstone of behavior change research and practice. Here, we examine the effect of learning about the behavior of outgroup members in a hyper-polarized context: mask-wearing during the COVID-19 pandemic. Contrary to prior findings, we find a descriptive social norm “backfire”: Across three experiments, Biden supporters increased their mask-wearing intentions after being informed that most Trump supporters never wore masks. We also provide evidence consistent with a mechanism whereby this effect was driven by changes in perceptions about how negatively ingroup members view nonmask wearing. Finally, in a fourth study, Biden supporters show the traditional descriptive norms effect (rather than a backfire) from the same treatment when in a nonpolarized context: dishonesty in a coin-flipping task. These findings help to clarify why descriptive social norm interventions promote behavior change, and underscore the importance of social norms in motivating prosocial behaviors. They also suggest an update to current best practices in the design of descriptive norm interventions: in polarized contexts, it can be beneficial to publicize antisocial behavior of outgroup members.
Testing the social pressure hypothesis: Does in-party social pressure reduce out-party empathy?
Authors: Pradella Lea 
Published: October 2024

Abstract
Empathy is considered one of the most critical components for bridging political divides and reducing animosity between political groups. Yet, empathy between political opponents is rare. There is a growing concern that partisans do not empathize with out-partisans because they feel social pressure from fellow in-partisans not to do so. This article examines this social pressure hypothesis and draws two conclusions. First, on the surface, the hypothesis seems plausible: citizens perceive fellow in-partisans as comparatively disapproving of and reluctant to engage in out-party empathy, and naïve cross-sectional analyses suggest that this perception translates into lower empathy towards out-partisans. Second, however, experimental data suggest that this relationship is not causal. Expecting disapproval from fellow in-party members for empathizing with out-partisans does not lead to a significant reduction in intentions to empathize with out-partisans. Rather, exploratory analyses suggest that social pressure by the in-party increases empathy toward out-partisans and triggers disappointment toward in-partisans. This implies that partisans can resist social pressure from the in-party and might even compensate for in-partisans’ lack of out-party empathy. The results are supported by original cross-sectional and experimental survey data (N=2,535) collected in the United States, an arguably most likely case for in-party social pressure to shape partisans’ intentions. The results have important implications for understanding the causes of and viable strategies for building empathy across political divides.
CODI: Enhancing machine learning-based molecular profiling through contextual out-of-distribution integration
Authors: Eissa Tarek , Huber Marinus , Obermayer-Pietsch Barbara , Linkohr Birgit , Peters Annette , Fleischmann Frank , Žigman Mihaela 
Published: October 2024

Abstract
Molecular analytics increasingly utilize machine learning (ML) for predictive modeling based on data acquired through molecular profiling technologies. However, developing robust models that accurately capture physiological phenotypes is challenged by the dynamics inherent to biological systems, variability stemming from analytical procedures, and the resource-intensive nature of obtaining sufficiently representative datasets. Here, we propose and evaluate a new method: Contextual Out-of-Distribution Integration (CODI). Based on experimental observations, CODI generates synthetic data that integrate unrepresented sources of variation encountered in real-world applications into a given molecular fingerprint dataset. By augmenting a dataset with out-of-distribution variance, CODI enables an ML model to better generalize to samples beyond the seed training data, reducing the need for extensive experimental data collection. Using three independent longitudinal clinical studies and a case–control study, we demonstrate CODI’s application to several classification tasks involving vibrational spectroscopy of human blood. We showcase our approach’s ability to enable personalized fingerprinting for multiyear longitudinal molecular monitoring and enhance the robustness of trained ML models for improved disease detection. Our comparative analyses reveal that incorporating CODI into the classification workflow consistently leads to increased robustness against data variability and improved predictive accuracy.
Standard Errors for Calibrated Parameters
Authors: Cocci Matthew D , Plagborg-Møller Mikkel 
Published: October 2024

Abstract
Calibration, the practice of choosing the parameters of a structural model to match certain empirical moments, can be viewed as minimum distance estimation. Existing standard error formulas for such estimators require a consistent estimate of the correlation structure of the empirical moments, which is often unavailable in practice. Instead, the variances of the individual empirical moments are usually readily estimable. Using only these variances, we derive conservative standard errors and confidence intervals for the structural parameters that are valid even under the worst-case correlation structure. In the over-identified case, we show that the moment weighting scheme that minimizes the worst-case estimator variance amounts to a moment selection problem with a simple solution. Finally, we develop tests of over-identifying or parameter restrictions. We apply our methods empirically to a model of menu cost pricing for multi-product firms and to a heterogeneous agent New Keynesian model.
Soluble urine activated leukocyte cell adhesion molecule is a strong predictor of lupus nephritis
Authors: Chu Dalena , Schwartz Noa , Ampudia Jeanette , Guthridge Joel , James Judith , Buyon Jill P , Connelly Stephen , Fung Maple , Ng Cherie T ,   The Accelerating Medicines Partnership: RA/SLE Network, Anolik Jennifer , Apruzzese William , Arazi Arnon , Berthier Celine , Brenner Michael , Buyon Jill , Clancy Robert , Connery Sean , Cunningham Melissa , Dall’Era Maria , Davidson Anne , Der Evan , Fava Andrea , Fonseka Chamith , Furie Richard , Goldman Dan , Gupta Rohit , Guthridge Joel , Hacohen Nir , Hildeman David , Hoover Paul , Hsu Raymond , James Judith , Kado Ruba , Kalunian Ken , Kamen Diane , Kretzler Mattias , Maecker Holden , Massarotti Elena , McCune William , McMahon Maureen , Park Meyeon , Payan-Schober Fernanda , Pendergraft William , Petri Michelle , Pichavant Mina , Putterman Chaim , Rao Deepak , Raychaudhuri Soumya , Slowikowski Kamil , Suryawanshi Hemant , Tuschl Thomas , Utz P J , Waguespack Dia , Wofsy David , Zhang Fan , Fava Andrea , Petri Michelle , Mohan Chandra , Putterman Chaim 
Published: October 2024

Abstract

Objectives
To evaluate urinary activated leucocyte cell adhesion molecule (ALCAM) and CD6 as predictors of LN progression or disease resolution across a 1-year study.

Methods
Serum and urine samples from biopsy proven LN subjects (n = 122) were prospectively collected over the course of a year at 3- or 6-month intervals (weeks 0, 12, 26 and 52) across multiple study sites and assessed for soluble ALCAM and CD6 levels. Urine creatinine from the same urine sample was used to normalize the levels of urinary ALCAM and urinary CD6. Measured levels of serum and urine ALCAM and CD6 were then analysed against disease metrics cross-sectionally and longitudinally.

Results
Cross-sectional analysis at baseline revealed that urinary ALCAM significantly correlated with urine protein creatinine ratio, renal SLEDAI, and the Physician Global Assessment (PGA), and negatively correlated with serum C3 and C4. Receiver operating characteristic curve analysis demonstrated that urinary ALCAM is a predictor of LN with an area under the curve (AUC) of 0.97, compared with urinary CD6 with an AUC of 0.71. Importantly, the change in urinary ALCAM over a 3-month period distinguished between non-responders and responders at week 52.

Conclusion
Urinary ALCAM is reflective of changes in LN and may be predictive of response status.
Neither increasing the frequency of sow feedings nor decreasing the interval between feedings prior to farrowing reduced piglet stillbirths
Authors: Johnson Danielle C , Perez Jeremy G , Estrada Jorge , Corzatt Deanne , Welch Michael W , Parr Eric , Boler Dustin D 
Published: October 2024

Abstract
Farrowing durations that exceed 240 min cause stillborn rates to increase. Therefore, feeding strategies in late gestation have been studied to mitigate the negative consequences of extended farrowing durations. A total of 1,501 sows (PIC 1050 Camborough) were used for this study at two individual farms near Carthage, IL. Farm 1 (758 sows) was a porcine reproductive and respiratory virus (PRRSv) stable (previously experienced a PRRSv outbreak and is currently vaccinated for PRRSv) with an older parity structure (3.67). Farm 2 (743 sows) was PRRSv positive (sows with pigs demonstrating Ct values &lt;36 determined by pig processing fluids) and had a younger parity structure (2.96). Sows were moved into farrowing rooms at approximately day 112 of gestation and started on their respective treatment. Treatment 1 sows were fed 1 meal of 2.27 kg per day at 0600 h. Treatment 2 sows were fed two equal meals of 1.13 kg (2.27 kg total) at 0600 and 1400 h. Treatment 3 sows were fed two equal meals of 1.13 kg (2.27 kg total) at 0600 and 1800 h. Treatment 4 sows were fed three equal meals of 0.77 kg (2.27 kg total) at 0600, 1400, and 2200 h. Treatments were assigned to farrowing rooms in a replicated 4 × 4 Latin Square arrangement of treatments where each treatment was fed in each room one time at each farm. Daily feed intakes were recorded from the time sows were introduced to the farrowing room until 5 d after farrowing. The total number of pigs born, pigs born alive, stillbirths, and mummies were recorded for each litter within 24 h of farrowing. Live pigs were weighed as a group to record litter birth weight. Pig mortality and morbidities were recorded until 5 d after farrowing. The total number of pigs born and pigs born alive were not different (P ≥ 0.59) among treatments. The number of stillborn pigs was not different (P = 0.33) among treatment 1(1.15 ± 1.41), treatment 2 (1.20 ± 1.36), treatment 3 (1.30 ± 1.46), and treatment 4 (1.14 ± 1.28). Sows fed three times per day at 8 h intervals tended (P = 0.08) to reduce the percentage of sows farrowed under supervision compared with sows fed once a day. Sows fed twice per day at 12 h intervals reduced (P = 0.01) the percentage of sows provided assistance compared with feeding sows once per day. Feeding a sow one meal of 2.27 kg, two meals (2.27 kg total), or three meals (2.27 kg total) of feed a day before farrowing did not reduce the number of stillborn piglets regardless of farm health status or parity structure.
Liquid chromatography–high-resolution mass spectrometry-based metabolomics revealing the effects of zearalenone and alpha-zearalenol on human endometrial cancer cells
Authors: Marhaba Marhaba , Nagendla Narendra Kumar , Anjum Saria , Ganneru Sireesha , Singh Varsha , Pal Saurabh , Mudiam Mohana Krishna Reddy , Ansari Kausar Mahmood 
Published: October 2024

Abstract
Human exposure to mycotoxins through food involve a mixture of compounds, which can be harmful to human health. The Fusarium fungal species are known to produce zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, and its metabolite alpha-zearalenol (α-ZEL), both of which possess endocrine-disruptive properties. Given their potential harm to human health through food exposure, investigating the combined effects of ZEN and α-ZEL becomes crucial. Hence, the combined impact of ZEN and α-ZEL study hold significant importance. This in vitro study delves into the critical area, examining their combined impact on the proliferation and metabolic profile of endometrial cancer Ishikawa cells via sulforhodamine, clonogenic, proliferating cell nuclear antigen (PCNA) and liquid chromatography-high resolution mass spectrometry (LC-HRMS) based untargeted metabolomics. Low concentrations of ZEN (25 nm), α-ZEL (10 nm), or a combination of both were observed to significantly enhance cell proliferation of Ishikawa cells, as evidenced by PCNA immunostaining, immunoblotting as well and clonogenic assays. The metabolomics revealed the perturbations in glycerophospholipid metabolism, nicotinate and nicotinamide metabolism and phenylalanine, tyrosine, tryptophan biosynthesis provides valuable insights into potential mechanism by which these mycotoxins may facilitate cell proliferation. However, further investigations are warranted to comprehensively understand the implications of these findings and their possible implications for human health.
4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) exposure induces hepatotoxicity and nephrotoxicity – role of oxidative stress, mitochondrial dysfunction and pathways of cytotoxicity
Authors: Maadurshni Gobichettipalayam Balasubramaniam , Nagarajan Manikandan , Mahalakshmi Balamurali , Sivasubramanian Jeganathan , Hemamalini Vedagiri , Manivannan Jeganathan 
Published: October 2024

Abstract

Objective
Bisphenol A (BPA) is a ubiquitous pollutant worldwide and 4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP) is considered a major active metabolite of BPA with a wide range of potent toxicological properties. However, its adverse outcome pathway (AOP) on the hepatic and renal system has not yet been explored.

Methods
Hence, the current study evaluated its effect on cell survival, oxidative stress, and apoptosis. In addition, the influence of signalling pathways on cytotoxicity and ROS generating enzymes (NOX2 and XO) on oxidative stress was explored by siRNA knockdown experiments. Further, its molecular interaction with SOD, CAT, and HSA (molecular docking and dynamics) was evaluated and validated with spectroscopy (fluorescence and FTIR) based methods.

Results
The outcome indicates that MBP exposure dose dependently increased the cytotoxic response, oxidative stress, and apoptosis in both hepatocytes and kidney cells. Further, MAPK signalling pathways and oxidative stress influenced the overall cytotoxic response in both cells. In addition, the stimulatory (NOX2 and XO) and inhibitory (SOD and CAT) effects of MBP were observed, along with a robust interaction with HSA.

Conclusions
The overall observation illustrates that MBP exposure adversely impacts hepatic and renal cells through oxidative stress and relevant molecular pathways which may connect the missing links during risk assessment of BPA.
Liver toxicity and repair evaluated by histopathology and electric modulus
Authors: Elwan Azhar M , Farag Ibrahim M , Elnasharty Mohamed M M 
Published: October 2024

Abstract
Detoxification is one of the most important liver functions. Therefore, liver is the front line of defense when the biosystem faces drug overdose, toxins, and anything that may cause harm. Some famous antibiotics are known for their side effects on liver; one of them is amoxicillin, AM. This work has investigated the toxic effect of amoxicillin on rat’s liver with overdose (90 mg/kg) and has studied the ameliorative role of protective and therapeutic Ashwagandha seeds extract (ASE) at doses (100, 200, and 300 mg/kg) against this toxicity. To achieve this work, the authors used two modalities; the first is liver histopathology to figure out the amoxicillin and ASE effects and to detect the sensitivity of another modality; the electric modulus, and its related thermodynamic parameters of liver tissue. Histopathological examination showed that the role of therapeutic ASE in reducing amoxicillin (AM) toxicity was more effective than the protective one. Also, most dielectric and thermodynamic results achieved the same result. Histopathology confirmed the liver injury by amoxicillin and the partial repair by the biosystem using ASE. Moreover, electric modulus, related dielectric parameters, and their thermodynamic state functions showed different changes in their values under the effect of amoxicillin. Using ASE helped the biosystem to restore these changes near their control values.