bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety-informed design: Using subgraph analysis to elicit hazardous emergent failure behavior in complex systems
Authors: McIntire Matthew G. , Hoyle Christopher , Tumer Irem Y. , Jensen David C. 
Published: October 2016

AbstractIdentifying failure paths and potentially hazardous scenarios resulting from component faults and interactions is a challenge in the early design process. The inherent complexity present in large engineered systems leads to nonobvious emergent behavior, which may result in unforeseen hazards. Current hazard analysis techniques focus on single hazards (fault trees), single faults (event trees), or lists of known hazards in the domain (hazard identification). Early in the design of a complex system, engineers may represent their system as a functional model. A function failure reasoning tool can then exhaustively simulate qualitative failure scenarios. Some scenarios can be identified as hazardous by hazard rules specified by the engineer, but the goal is to identify scenarios representing unknown hazards. The incidences of specific subgraphs in graph representations of known hazardous scenarios are used to train a classifier to distinguish hazard from nonhazard. The algorithm identifies the scenario most likely to be hazardous, and presents it to the engineer. After viewing the scenario and judging its safety, the engineer may have insight to produce additional hazard rules. The collaborative process of strategic presentation of scenarios by the computer and human judgment will identify previously unknown hazards. The feasibility of this methodology has been tested on a relatively simple functional model of an electrical power system with positive results. Related work applying function failure reasoning to a team of robotic rovers will provide data from a more complex system.
Neuropsychological Profile of Lifetime Traumatic Brain Injury in Older Veterans
Authors: Kaup Allison R. , Peltz Carrie , Kenney Kimbra , Kramer Joel H. , Diaz-Arrastia Ramon , Yaffe Kristine 
Published: October 2016

AbstractObjectives:The aim of this study was to characterize the neuropsychological profile of lifetime traumatic brain injury (TBI) in older Veterans.Methods:Participants were 169 older Veterans [mean age=79.1 years (range, 51–97 years), 89% male, 92% Caucasian], 88 with lifetime TBI and 81 without TBI, living in Veterans’ retirement homes in independent residence. TBI history was ascertained with the Ohio State TBI Identification Method structured interview. Cognition was assessed with neuropsychological tests: Raw scores were converted toZ-scores compared to age-corrected normative data and combined into five domain compositeZ-scores (attention/working memory, learning/memory, language, processing speed, executive functioning). We investigated the association between TBI and performance in each cognitive domain in linear mixed effects models, with and without adjustment for demographics, medical comorbidities, and psychiatric variables.Results:Compared to those without TBI, older Veterans with TBI had greater deficits in processing speed (estimate=−.52;p=.01;f2=.08 in fully adjusted model) and executive functioning (estimate=−.41;p=.02;f2=.06 in fully adjusted model) but performed similarly in the attention/working memory, learning/memory, and language domains (allp>.05). TBI-associated deficits were most prominent among individuals with multiple mild TBIs and those with any moderate-to-severe TBI, but were not clearly present among those with single mild TBI.Conclusions:The neuropsychological profile of lifetime TBI in older Veterans is characterized by slowed processing speed and executive dysfunction, especially among those with greater injury burden. This pattern may reflect long-standing deficits or a TBI-associated cognitive decline process distinct from Alzheimer’s disease. (JINS, 2017,23, 56–64)
Is the Law an Ass When It Comes to Mules? How Indonesia Can Lead a New Global Approach to Treating Drug Traffickers as Human Trafficked Victims
Authors: GERRY QC Felicity , HARRÉ Thomas , NAIBAHO Nathalina , MURASZKIEWICZ Julia , BOISTER Neil 
Published: October 2016

AbstractIndonesia has enacted laws which provide mandatory protection for victims of human trafficking. It also has mandatory drug laws which, in some cases, lead to the death penalty. This legislative conflict together with investigative and prosecutorial failure risks the execution of human trafficked victims who are used as drug mules in organized crime. In countries where there is no statutory defence to criminal conduct, there is a need to approach criminal conduct in a way that protects victims. This includes mechanisms to ensure non-prosecution and non-punishment. The recent reprieve for Mary Jane Veloso, albeit temporary at the time of writing, is an opportunity for Indonesia to lead a new global approach to victim protection.
Divergent effects of central melanocortin signalling on fat and sucrose preference in humans
Authors: van der Klaauw Agatha A. , Keogh Julia M. , Henning Elana , Stephenson Cheryl , Kelway Sarah , Trowse Victoria M. , Subramanian Naresh , O’Rahilly Stephen , Fletcher Paul C. , Farooqi I. Sadaf 
Published: October 2016

AbstractMelanocortin-4-receptor (MC4R)-expressing neurons modulate food intake and preference in rodents but their role in human food preference is unknown. Here we show that compared with lean and weight-matched controls, MC4R deficient individuals exhibited a markedly increased preference for high fat, but a significantly reduced preference for high sucrose food. These effects mirror those in Mc4r null rodents and provide evidence for a central molecular circuit influencing human macronutrient preference.
Up-regulation of miR-95-3p in hepatocellular carcinoma promotes tumorigenesis by targeting p21 expression
Authors: Ye Jian , Yao Yufeng , Song Qixue , Li Sisi , Hu Zhenkun , Yu Yubing , Hu Changqing , Da Xingwen , Li Hui , Chen Qiuyun , Wang Qing K. 
Published: October 2016

AbstractHepatocellular carcinoma (HCC) is one of the most common malignant cancers. To elucidate new regulatory mechanisms for heptocarcinogenesis, we investigated the regulation of p21, a cyclin-dependent kinase (CDK) inhibitor encoded by CDKN1A, in HCC. The expression level of p21 is decreased with the progression of HCC. Luciferase assays with a luciferase-p21-3′ UTR reporter and its serial deletions identified a 15-bp repressor element at the 3′-UTR of CDKN1A, which contains a binding site for miR-95-3p. Mutation of the binding site eliminated the regulatory effect of miR-95-3p on p21 expression. Posttranscriptional regulation of p21 expression by miR-95-3p is mainly on the protein level (suppression of translation). Overexpression of miR-95-3p in two different HCC cell lines, HepG2 and SMMC7721, significantly promoted cell proliferation, cell cycle progression and cell migration, whereas a miR-95-3p specific inhibitor decreased cell proliferation, cell cycle progression and cell migration. The effects of miR-95-3p on cellular functions were rescued by overexpression of p21. Overexpression of miR-95-3p promoted cell proliferation and tumor growth in HCC xenograft mouse models. Expression of miR-95-3p was significantly higher in HCC samples than in adjacent non-cancerous samples. These results demonstrate that miR-95-3p is a potential new marker for HCC and regulates hepatocarcinogenesis by directly targeting CDKN1A/p21 expression.
Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage
Authors: Jurczak-Kurek Agata , Gąsior Tomasz , Nejman-Faleńczyk Bożena , Bloch Sylwia , Dydecka Aleksandra , Topka Gracja , Necel Agnieszka , Jakubowska-Deredas Magdalena , Narajczyk Magdalena , Richert Malwina , Mieszkowska Agata , Wróbel Borys , Węgrzyn Grzegorz , Węgrzyn Alicja 
Published: October 2016

AbstractA large scale analysis presented in this article focuses on biological and physiological variety of bacteriophages. A collection of 83 bacteriophages, isolated from urban sewage and able to propagate in cells of different bacterial hosts, has been obtained (60 infecting Escherichia coli, 10 infecting Pseudomonas aeruginosa, 4 infecting Salmonella enterica, 3 infecting Staphylococcus sciuri, and 6 infecting Enterococcus faecalis). High biological diversity of the collection is indicated by its characteristics, both morphological (electron microscopic analyses) and biological (host range, plaque size and morphology, growth at various temperatures, thermal inactivation, sensitivity to low and high pH, sensitivity to osmotic stress, survivability upon treatment with organic solvents and detergents), and further supported by hierarchical cluster analysis. By the end of the research no larger collection of phages from a single environmental source investigated by these means had been found. The finding was confirmed by whole genome analysis of 7 selected bacteriophages. Moreover, particular bacteriophages revealed unusual biological features, like the ability to form plaques at low temperature (4 °C), resist high temperature (62 °C or 95 °C) or survive in the presence of an organic solvents (ethanol, acetone, DMSO, chloroform) or detergent (SDS, CTAB, sarkosyl) making them potentially interesting in the context of biotechnological applications.
Quantifying yield behaviour in metals by X-ray nanotomography
Authors: Mostafavi M. , Bradley R. , Armstrong D. E. J. , Marrow T. J. 
Published: October 2016

AbstractNanoindentation of engineering materials is commonly used to study, at small length scales, the continuum mechanical properties of elastic modulus and yield strength. However, it is difficult to measure strain hardening via nanoindentation. Strain hardening, which describes the increase in strength with plastic deformation, affects fracture toughness and ductility, and is an important engineering material property. The problem is that the load-displacement data of a single nanoindentation do not provide a unique solution for the material’s plastic properties, which can be described by its stress-strain behaviour. Three-dimensional mapping of the displacement field beneath the indentation provides additional information that can overcome this difficulty. We have applied digital volume correlation of X-ray nano-tomographs of a nanoindentation to measure the sub-surface displacement field and so obtain the plastic properties of a nano-structured oxide dispersion strengthened steel. This steel has potential applications in advanced nuclear energy systems, and this novel method could characterise samples where proton irradiation of the surface simulates the effects of fast neutron damage, since facilities do not yet exist that can replicate this damage in bulk materials.
The polarization modulation and fabrication method of two dimensional silica photonic crystals based on UV nanoimprint lithography and hot imprint
Authors: Guo Shuai , Niu Chunhui , Liang Liang , Chai Ke , Jia Yaqing , Zhao Fangyin , Li Ya , Zou Bingsuo , Liu Ruibin 
Published: October 2016

AbstractBased on a silica sol-gel technique, highly-structurally ordered silica photonic structures were fabricated by UV lithography and hot manual nanoimprint efforts, which makes large-scale fabrication of silica photonic crystals easy and results in low-cost. These photonic structures show perfect periodicity, smooth and flat surfaces and consistent aspect ratios, which are checked by scanning electron microscopy (SEM) and atomic force microscopy (AFM). In addition, glass substrates with imprinted photonic nanostructures show good diffraction performance in both transmission and reflection mode. Furthermore, the reflection efficiency can be enhanced by 5 nm Au nanoparticle coating, which does not affect the original imprint structure. Also the refractive index and dielectric constant of the imprinted silica is close to that of the dielectric layer in nanodevices. In addition, the polarization characteristics of the reflected light can be modulated by stripe nanostructures through changing the incident light angle. The experimental findings match with theoretical results, making silica photonic nanostructures functional integration layers in many optical or optoelectronic devices, such as LED and microlasers to enhance the optical performance and modulate polarization properties in an economical and large-scale way.
Tunable sound transmission at an impedance-mismatched fluidic interface assisted by a composite waveguide
Authors: Zhang Hui , Wei Zhi , Fan Li , Qu Jianmin , Zhang Shu-yi 
Published: October 2016

AbstractWe report a composite waveguide fabricated by attaching a coupling aperture to a waveguide. The acoustic impedance of the composite waveguide can be regulated by merely controlling its coupling vibrations, depending on its structure size. By changing the size to adjust the acoustic impedance of the composite waveguide at an impedance-mismatched fluidic interface, tunable sound transmission at the desired frequencies is achieved. The reported composite waveguide provides a new method for sound regulation at a mismatched fluidic interface and has extensive frequency hopping and frequency agility applications in air-water sound communication.
Sleep duration, daytime napping, markers of obstructive sleep apnea and stroke in a population of southern China
Authors: Wen Ye , Pi Fu-Hua , Guo Pi , Dong Wen-Ya , Xie Yu-Qing , Wang Xiang-Yu , Xia Fang-Fang , Pang Shao-Jie , Wu Yan-Chun , Wang Yuan-Yuan , Zhang Qing-Ying 
Published: October 2016

AbstractSleep habits are associated with stroke in western populations, but this relation has been rarely investigated in China. Moreover, the differences among stroke subtypes remain unclear. This study aimed to explore the associations of total stroke, including ischemic and hemorrhagic type, with sleep habits of a population in southern China. We performed a case-control study in patients admitted to the hospital with first stroke and community control subjects. A total of 333 patients (n = 223, 67.0%, with ischemic stroke; n = 110, 23.0%, with hemorrhagic stroke) and 547 controls were enrolled in the study. Participants completed a structured questionnaire to identify sleep habits and other stroke risk factors. Least absolute shrinkage and selection operator (Lasso) and multiple logistic regression were performed to identify risk factors of disease. Incidence of stroke, and its subtypes, was significantly associated with snorting/gasping, snoring, sleep duration, and daytime napping. Snorting/gasping was identified as an important risk factor in the Lasso logistic regression model (Lasso’ β = 0.84), and the result was proven to be robust. This study showed the association between stroke and sleep habits in the southern Chinese population and might help in better detecting important sleep-related factors for stroke risk.
Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis
Authors: Tanigawa Hiroki , Miyata Keishi , Tian Zhe , Aoi Jun , Kadomatsu Tsuyoshi , Fukushima Satoshi , Ogata Aki , Takeda Naoki , Zhao Jiabin , Zhu Shunshun , Terada Kazutoyo , Endo Motoyoshi , Morinaga Jun , Sugizaki Taichi , Sato Michio , Morioka Masaki Suimye , Manabe Ichiro , Mashimo Youichi , Hata Akira , Taketomi Yoshitaka , Yamamoto Kei , Murakami Makoto , Araki Kimi , Jinnin Masatoshi , Ihn Hironobu , Oike Yuichi 
Published: October 2016

AbstractPsoriasis is a chronic inflammatory skin disease marked by aberrant tissue repair. Mutant mice modeling psoriasis skin characteristics have provided useful information relevant to molecular mechanisms and could serve to evaluate therapeutic strategies. Here, we found that epidermal ANGPTL6 expression was markedly induced during tissue repair in mice. Analysis of mice overexpressing ANGPTL6 in keratinocytes (K14-Angptl6 Tg mice) revealed that epidermal ANGPTL6 activity promotes aberrant epidermal barrier function due to hyperproliferation of prematurely differentiated keratinocytes. Moreover, skin tissues of K14-Angptl6 Tg mice showed aberrantly activated skin tissue inflammation seen in psoriasis. Levels of the proteins S100A9, recently proposed as therapeutic targets for psoriasis, also increased in skin tissue of K14-Angptl6 Tg mice, but psoriasis-like inflammatory phenotypes in those mice were not rescued by S100A9 deletion. This finding suggests that decreasing S100A9 levels may not ameliorate all cases of psoriasis and that diverse mechanisms underlie the condition. Finally, we observed enhanced levels of epidermal ANGPTL6 in tissue specimens from some psoriasis patients. We conclude that the K14-Angptl6 Tg mouse is useful to investigate psoriasis pathogenesis and for preclinical testing of new therapeutics. Our study also suggests that ANGPTL6 activation in keratinocytes enhances psoriasis susceptibility.
Metabolomics reveals the mechanisms for the cardiotoxicity of Pinelliae Rhizoma and the toxicity-reducing effect of processing
Authors: Su Tao , Tan Yong , Tsui Man-Shan , Yi Hua , Fu Xiu-Qiong , Li Ting , Chan Chi Leung , Guo Hui , Li Ya-Xi , Zhu Pei-Li , Tse Anfernee Kai Wing , Cao Hui , Lu Ai-Ping , Yu Zhi-Ling 
Published: October 2016

AbstractPinelliae Rhizoma (PR) is a commonly used Chinese medicinal herb, but it has been frequently reported about its toxicity. According to the traditional Chinese medicine theory, processing can reduce the toxicity of the herbs. Here, we aim to determine if processing reduces the toxicity of raw PR, and to explore the underlying mechanisms of raw PR-induced toxicities and the toxicity-reducing effect of processing. Biochemical and histopathological approaches were used to evaluate the toxicities of raw and processed PR. Rat serum metabolites were analyzed by LC-TOF-MS. Ingenuity pathway analysis of the metabolomics data highlighted the biological pathways and network functions involved in raw PR-induced toxicities and the toxicity-reducing effect of processing, which were verified by molecular approaches. Results showed that raw PR caused cardiotoxicity, and processing reduced the toxicity. Inhibition of mTOR signaling and activation of the TGF-β pathway contributed to raw PR-induced cardiotoxicity, and free radical scavenging might be responsible for the toxicity-reducing effect of processing. Our data shed new light on the mechanisms of raw PR-induced cardiotoxicity and the toxicity-reducing effect of processing. This study provides scientific justifications for the traditional processing theory of PR, and should help in optimizing the processing protocol and clinical combinational application of PR.
Printed 2 V-operating organic inverter arrays employing a small-molecule/polymer blend
Authors: Shiwaku Rei , Takeda Yasunori , Fukuda Takashi , Fukuda Kenjiro , Matsui Hiroyuki , Kumaki Daisuke , Tokito Shizuo 
Published: October 2016

AbstractPrinted organic thin-film transistors (OTFTs) are well suited for low-cost electronic applications, such as radio frequency identification (RFID) tags and sensors. Achieving both high carrier mobility and uniform electrical characteristics in printed OTFT devices is essential in these applications. Here, we report on printed high-performance OTFTs and circuits using silver nanoparticle inks for the source/drain electrodes and a blend of dithieno[2,3-d;2′,3′-d′]benzo[1,2-b;4,5-b′]dithiophene (DTBDT-C6) and polystyrene for the organic semiconducting layer. A high saturation region mobility of 1.0 cm2 V−1 s−1at low operation voltage of −5 V was obtained for relatively short channel lengths of 9 μm. All fifteen of the printed pseudo-CMOS inverter circuits were formed on a common substrate and operated at low operation voltage of 2 V with the total variation in threshold voltage of 0.35 V. Consequently, the printed OTFT devices can be used in more complex integrated circuit applications requiring low manufacturing cost over large areas.
Brain-derived neurotrophic factor protects against tau-related neurodegeneration of Alzheimer’s disease
Authors: Jiao S-S , Shen L-L , Zhu C , Bu X-L , Liu Y-H , Liu C-H , Yao X-Q , Zhang L-L , Zhou H-D , Walker D G , Tan J , Götz J , Zhou X-F , Wang Y-J 
Published: October 2016

AbstractReduced expression of brain-derived neurotrophic factor (BDNF) has a crucial role in the pathogenesis of Alzheimer’s disease (AD), which is characterized with the formation of neuritic plaques consisting of amyloid-beta (Aβ) and neurofibrillary tangles composed of hyperphosphorylated tau protein. A growing body of evidence indicates a potential protective effect of BDNF against Aβ-induced neurotoxicity in AD mouse models. However, the direct therapeutic effect of BDNF supplement on tauopathy in AD remains to be established. Here, we found that the BDNF level was reduced in the serum and brain of AD patients and P301L transgenic mice (a mouse model of tauopathy). Intralateral ventricle injection of adeno-associated virus carrying the gene encoding human BDNF (AAV-BDNF) achieved stable expression of BDNF gene and restored the BDNF level in the brains of P301L mice. Restoration of the BDNF level attenuated behavioral deficits, prevented neuron loss, alleviated synaptic degeneration and reduced neuronal abnormality, but did not affect tau hyperphosphorylation level in the brains of P301L mice. Long-term expression of AAV-BDNF in the brain was well tolerated by the mice. These findings suggest that the gene delivery of BDNF is a promising treatment for tau-related neurodegeneration for AD and other neurodegenerative disorders with tauopathy.
Cancer cells enter dormancy after cannibalizing mesenchymal stem/stromal cells (MSCs)
Authors: Bartosh Thomas J. , Ullah Mujib , Zeitouni Suzanne , Beaver Joshua , Prockop Darwin J. 
Published: October 2016

SignificanceIn many patients with cancer, some tumor cells tolerate conventional treatments and persist for years in an undetectable/dormant state, after which these same cells can mysteriously resume their growth and seed, almost invariably fatal, recurrent cancerous lesions. The therapeutic challenges of tumor dormancy and need to decode the underlying mechanisms involved are apparent. Here, we revealed that mesenchymal stem/stromal cells (MSCs), recognized determinants of breast cancer cell (BCC) behavior, were readily cannibalized by the BCCs they mingled with in 3D cocultures, a process that distinctly altered cancer cell phenotype, suppressed tumor formation, and supported tumor dormancy. Our discoveries provide original insight into the interactions between MSCs and cancer cells, with the potential to identify novel molecular targets for cancer therapy.
AoAtg26, a putative sterol glucosyltransferase, is required for autophagic degradation of peroxisomes, mitochondria, and nuclei in the filamentous fungus Aspergillus oryzae
Authors: Kikuma Takashi , Tadokoro Takayuki , Maruyama Jun-ichi , Kitamoto Katsuhiko 
Published: October 2016

Abstract
Autophagy is a conserved process in eukaryotic cells for degradation of cellular proteins and organelles. In filamentous fungi, autophagic degradation of organelles such as peroxisomes, mitochondria, and nuclei occurs in basal cells after the prolonged culture, but its mechanism is not well understood. Here, we functionally analyzed the filamentous fungus Aspergillus oryzae AoAtg26, an ortholog of the sterol glucosyltransferase PpAtg26 involved in pexophagy in the yeast Pichia pastoris. Deletion of Aoatg26 caused a severe decrease in conidiation and aerial hyphae formation, which is typically observed in the autophagy-deficient A. oryzae strains. In addition, cup-shaped AoAtg8-positive membrane structures were accumulated in the Aoatg26 deletion strain, indicating that autophagic process is impaired. Indeed, the Aoatg26 deletion strain was defective in the degradation of peroxisomes, mitochondria, and nuclei. Taken together, AoAtg26 plays an important role for autophagic degradation of organelles in A. oryzae, which may physiologically contribute to the differentiation in filamentous fungi.