bluuebunny/crossref_metadata_2025_split huggingface dataset:
The dimerization domain of SARS CoV 2 Nucleocapsid protein is partially disordered as a monomer and forms a high affinity dynamic complex
Authors: Cubuk Jasmine , Jeremias Incicco J. , Hall Kathleen B. , Holehouse Alex S. , Stuchell-Brereton Melissa D. , Soranno Andrea 
Published: September 2024

AbstractThe SARS-CoV-2 Nucleocapsid (N) is a 419 amino acids protein that drives the compaction and packaging of the viral genome. This compaction is aided not only by protein-RNA interactions, but also by protein-protein interactions that contribute to increasing the valence of the nucleocapsid protein. Here, we focused on quantifying the mechanisms that control dimer formation. Single-molecule Förster Resonance Energy Transfer enabled us to investigate the conformations of the dimerization domain in the context of the full-length protein as well as the energetics associated with dimerization. Under monomeric conditions, we observed significantly expanded configurations of the dimerization domain (compared to the folded dimer structure), which are consistent with a dynamic conformational ensemble. The addition of unlabeled protein stabilizes a folded dimer configuration with a high mean transfer efficiency, in agreement with predictions based on known structures. Dimerization is characterized by a dissociation constant of ∼ 12 nM at 23OC and is driven by strong enthalpic interactions between the two protein subunits, which originate from the coupled folding and binding. Interestingly, the dimer structure retains some of the conformational heterogeneity of the monomeric units, and the addition of denaturant reveals that the dimer domain can significantly expand before being completely destabilized. Our findings suggest that the inherent flexibility of the monomer form is required to adopt the specific fold of the dimer domain, where the two subunits interlock with one another. We proposed that the retained flexibility of the dimer form may favor the capture and interactions with RNA, and that the temperature dependence of dimerization may explain some of the previous observations regarding the phase separation propensity of the N protein.
An Effective Surface Passivation Assay for Single-Molecule Studies of Chromatin and Topoisomerase II
Authors: Le Tung T. , Gao Xiang , Park Seong Ha , Lee Jaeyoon , Inman James T. , Wang Michelle D. 
Published: September 2024

SUMMARYFor single-molecule studies requiring surface anchoring of biomolecules, a poorly passivated surface can result in alterations of biomolecule structure and function that can result in artifacts. This protocol describes surface passivation and sample chamber preparation for mechanical manipulation of chromatin fibers and characterization of topoisomerase II activity in physiological buffer conditions. The method employs enhanced surface hydrophobicity and purified blocking proteins to reduce non-specific surface adsorption. This method is accessible, cost-effective, and potentially widely applicable to other biomolecules.For a complete list of publications that employ this protocol, see the paper references.B.GRAPHICAL ABSTRACT
The nexus between institutional quality and the financial sustainability of microfinance institutions
Authors: Tehulu Tilahun Aemiro , Muhammed Shekur Wosen , Melesse Mesfin Teshager 
Published: September 2024

PurposeIn recent years, researchers have shown an increased interest in studying the institutional environment–financial institutions’ performance nexus. However, little attention is paid to investigating the role of institutional quality in the financial sustainability of microfinance institutions (MFIs). Consequently, this study explores whether investments in strengthening institutional environment enhance MFIs’ financial sustainability.Design/methodology/approachThe study relies on an unbalanced panel dataset of 136/138 MFIs in Sub-Saharan Africa (SSA) spanning from 2004 to 2018, which was obtained from the Microfinance Information Exchange (MIX) Market database under the World Bank catalog. Data for institutional factors are accessed from the World Bank database for World Governance Indicators (WGI). The study applies the two-step system generalized method of moments (GMM) to analyze the data.FindingsThe research uncovers that institutional environment matters in the financial sustainability of MFIs. The study shows that institutional quality is, in the aggregate, positively associated with financial sustainability. Different institutional factors also have distinct impacts on financial sustainability. While contemporaneous relationships are discovered between government effectiveness (GOVE), rule of law (RUL) and sustainability, the relationship between control of corruption (CCOR) and sustainability is an intertemporal one. Unlike the others, CCOR impacts sustainability with a one-year lag and not instantaneously. Nevertheless, the effects of the aforementioned institutional factors on financial sustainability are all positive and consistent with the result for the aggregate measure.Practical implicationsThe practical implication of our findings to MFI managers is that strategies should be developed and instituted to manage MFI-specific factors appropriately and counterbalance the negative effect of a weak institutional environment (in SSA) on financial sustainability, as MFIs have no or less control over the institutional quality. For policymakers, our findings underscore the significance of policy documents that assist developing economies in improving their institutional environment, as strong institutions are vital for MFIs in the attainment of financial sustainability, which is crucial for sustainable poverty reduction.Originality/valueWhile the extant literature provides valuable insights that different MFI-specific factors drive the financial sustainability of MFIs, the previous studies fail to address the role of institutional quality in the financial sustainability of MFIs. This study examines the nexus between institutional quality and financial sustainability, which has been ignored in the previous literature.
Analyzing the impact of digital innovation ecosystem on the intelligent development in high-end equipment manufacturing industry: a dynamic QCA analysis
Authors: Liu Yujing , Li Meifang 
Published: September 2024

PurposeThis study explores how the high-end equipment manufacturing industry (HEMI) achieves intelligent development through the digital innovation ecosystem. While this industry urgently needs to achieve intelligent development through innovation breakthroughs, existing research lacks a deep analysis in conjunction with the digital innovation ecosystem. Considering the sophisticated nature of HEMI and the unique characteristics of the digital innovation ecosystem, this paper aims to uncover the innovation potential and synergetic development opportunities that arise from their integration.Design/methodology/approachThis study uses Dynamic Qualitative Comparative Analysis (QCA) to explore the evolving relationship between the digital innovation ecosystem and intelligent development in HEMI enterprises. Data from 60 HEMI enterprises were collected from 2015 to 2022, and the study window was divided into two-year intervals for analysis. Compared to traditional QCA methods, this approach overcomes the limitations of cross-sectional analysis, fully accounting for time’s influence on causal relationships for more accurate results.FindingsThe study reveals that the digital innovation ecosystem of HEMI drives intelligent development through the coordinated interactions of its elements within each time window. Configuration paths and key driving factors evolve dynamically, reflecting the complexity of the ecosystem’s role in driving intelligent development. The study suggests that enterprises dynamically adjust their strategies to different stages, enhancing the effectiveness of intelligent transformation.Originality/valueThe paper proposes and validates a digital innovation ecosystem framework for HEMI, systematically exploring its role in driving intelligent development. The study fills a research gap and extends innovation ecosystem theory by identifying core driving factors and their evolutionary trends through Dynamic QCA. It offers a new perspective on the dynamic role of digital innovation ecosystems in intelligent transformation.
You reap what you sow – abusive leadership impact on organizational digital innovation through knowledge workers' competence and productivity
Authors: Sumbal Muhammad Saleem , Sahibzada Umar Farooq , Ahmed Quba , Russo Francesco , Papa Armando 
Published: September 2024

PurposeThis research aims to determine how abusive leadership negatively affects knowledge workers' productivity and its potential influence on digital innovation. The present study also explores the mediating role of a knowledge worker’s competence between abusive leadership and knowledge worker productivity.Design/methodology/approachThe study employed a quantitative research approach. Data were gathered through purposive sampling using 255 questionnaires completed by IT industry knowledge workers in Pakistan. The data were analyzed using the SMART-PLS 4.0 software.FindingsThe findings demonstrated a negative correlation between abusive leadership and knowledge worker productivity. Additionally, the relationship was partially mediated by knowledge worker competence. Furthermore, the study asserts that knowledge worker productivity has a substantial positive impact on digital innovation.Originality/valueThis study contributes substantially to the existing body of evidence on the productivity of knowledge workers and digital innovation by examining the interlocking effects of abusive leadership. It also implies the interpersonal mechanism of employee competence that connects abusive leadership with the productivity of knowledge workers and digital innovation. Thus, this study is one of the first inquiries to analyze this paradigm.
Innovative work behavior of intrapreneurs: a matter of belonging and support?
Authors: Berisha Gentrit , Govori Drin , Lajçi Rrezon , Sonta Monika , Röhm Thomas 
Published: September 2024

PurposeThe manager is to innovation what innovation is to organizations. Driven by this axiom, researchers and practitioners have turned their attention to the drivers of innovative work behavior of managers. In this study, we investigate whether organizational-based job attitudes (workplace belongingness and management support) are related to the innovative work behavior of managers directly or through the underlying mechanism of intrapreneurial intention.Design/methodology/approachData were gathered from 201 managers working in various industries in Kosovo. Using structural equation modeling, we tested how management support (MS) and workplace belongingness (WB) are related to innovative work behavior (IWB) as a direct relationship and mediated by intrapreneurial intentions (INI).FindingsThe study's findings reveal that the relationship between workplace belongingness and innovative work behavior is positive and significant. The relationship between management support and innovative work behavior is insignificant, but intrapreneurial intention fully mediates this relationship. The strongest relationship attested is between intrapreneurial intention and innovative work behavior, supporting the attitude-intention-behavior nexus.Research limitations/implicationsOur study is limited to a single-country, cross-sectional study, which hinders generalization and is susceptible to reverse causation.Originality/valueOur study sheds light on the attitude-intention-behavior chain and provides insights for fostering innovative work behavior among managers. This is the first study to test the influence of management support and workplace belongingness as attitudes on innovative work behavior of managers from the lens of place attachment theory and perceived organizational support theory.
The effect of demographic shift on property values in Japan
Authors: Peng Ti-Ching 
Published: September 2024

Purpose
Demographic transition, although gradual, has a profound impact on various socio-economic aspects of society, including the real estate market. Many countries, particularly Japan, are currently experiencing rapid population aging. While the rising participation of seniors in the labour market has helped to alleviate this demographic burden in Western societies, the question is whether, in Japan, the purpose of this paper is to examine if the return of elderly individuals to the workforce could mitigate the demographic pressures on the housing sector. Besides the conventional panel model, this study used the quantile panel method to evaluate whether the effect of this demographic shift remains consistent across property value ranges.

Design/methodology/approach
This study considered a panel data set of 47 prefectures from 2006 to 2016 in Japan, focusing on weighted dependency ratios that account for the labour force participation rates of three elder age cohorts. This approach captures the shifting dynamics of the workforce, particularly as more Japanese retirees have re-entered the labour market in recent years. By applying the quantile panel model (Koenker, 2004), this paper explores whether the reduced demographic burden exerts varying impacts across different quantiles of property prices.

Findings
The conventional and two weighted dependency ratios revealed their non-linear influence on different quantiles of property values in quantile panel models. The return of elderly labourers indeed mitigated the demographic burden given the weighted dependency ratios’ weaker negative influence on the property prices at Q90, compared to the conventional dependency ratio. However, compared to the conventional dependency ratio, the stronger positive effects of weighted dependency ratios on the prices at Q10 imply that the demographic debt associated with the working involvement of these inferior elders may pressure people, especially elders, to favour and buy cheap residences.

Originality/value
The return of retirees to the workforce should be a good sign, given the shrinking labour force in Japan. As one of the few studies considering this demographic shift in measuring dependency ratios, this paper further evaluates its effect on different quantiles of property values in Japan. Unlike their Western counterparts, many seniors in Japan work out of financial necessity. It is found in this study that the demographic debt associated with the working involvement of inferior seniors may pressure people to favour cheap residences. These findings should give policymakers a better understanding of the housing market in this aging era.
Systematic literature review and retrospective bibliometric analysis on ESG research
Authors: Mukhtar Bilal , Shad Muhammad Kashif , Ali Kashif , Woon Lai Fong , Waqas Ahmad 
Published: September 2024

PurposeThis study aims to holistically present a systematic literature review (SLR) triangulated with bibliometric analysis on environmental, social and governance (ESG) research to synthesize and comprehensively review its evolving journey and emerging research streams.Design/methodology/approachUsing R-studio software, this study carried out a retrospective quantitative bibliometric analysis through performance analysis, science mapping and network analysis, covering 261 documents published on ESG research between 2007 and 2022 in Scopus and Web of Science databases.FindingsPerformance analysis depicts the trends in publications, impactful journals and influential publications, authors and countries, while science mapping incorporates co-words and thematic analysis. Likewise, co-occurrence analysis provided four different clusters, representing ESG research linkage to other management fields along with key insights from co-citation network analysis. Additionally, the theory–context–characteristics–methods (TCCM) framework has provided valuable results in terms of widely and emerging used theories, contexts, characteristics and methodologies in ESG research.Research limitations/implicationsThe findings of this study’s comprehensive bibliometric analysis combined with SLR uncovered a robust roadmap for further investigation in ESG research by identifying the inherent structure and evolution of research themes. This review has not only identified the prevalent gaps in determining priorities for future research but also provides insights which not previously been captured and evaluated on this topic.Originality/valueTo the best of the author's knowledge, no study presents the TCCM framework in the context of bibliometric analysis of ESG research. Besides, a conceptual framework is developed that illustrates antecedents, mediators, moderators and outcomes of research on ESG practices and provides the concluded key takeaways and recommendations for potential authors intending to publish their research papers on ESG practices.
Religiosity and positive emotions as predictors of work engagement among academics in Algeria
Authors: Elkheloufi Abderrahmane , Benlahcene Abderrahmane , Al Ansi Abdullah M. , Queiri Abdelbaset , Yean Tan Fee 
Published: September 2024

PurposeIntegration of religiosity and positive emotions at workplace sheds light on the intersection of faith and professional well-being where these two factors lead to increased job satisfaction, higher productivity and improved overall mental health. The study examines the relationship between religiosity, positive emotions and work engagement among academics in Algeria.Design/methodology/approachThis research is based on the Conservation of Resources (COR) theory and the job demands-resources (JD-R) model of work engagement. The study uses a cross-sectional self-administered questionnaire with stratified random sampling to collect data from 356 academics at public universities in Algeria. The data were analysed using partial least squares structural equation modelling (PLS-SEM) with Smart PLS 3.FindingsThe results indicate that religiosity is positively associated with work engagement, and positive emotions mediate the relationship between religiosity and work engagement. The study also illustrates that job demands (workload) moderate the relationship between positive emotions and work engagement.Originality/valueThe study highlighted that role of religiosity and positive emotions as essential factors in increasing academics' work engagement and contributes to the COR theory and JD-R model of work engagement.
The causal factors of transformational leadership and sufficiency economy philosophy practice influencing the performance of coast tourism business in Thailand
Authors: Suttipun Muttanachai 
Published: September 2024

Purpose Transactional leadership and old-school management practices may not answer the changes in today’s world. Therefore, the study aimed to examine the influences of transformational leadership and the practice of sufficiency economy philosophy (SEP) on the performance of the coast tourism business in Thailand.Design/methodology/approach The population of this study comprised all Thai coast tourism businesses. Using simple random sampling, the quantitative data were collected from 407 coast tourism businesses in Thailand as the samples. Transformational leadership, SEP practice and business performance were adapted from the literature. Path analysis and factor confirmation including a correlation matrix were used to test the causal factors influencing the Thai coast tourism business’s performance.Findings Transformational leadership, SEP practice and performance of coast tourism business in Thailand were on a high level. Moreover, there were positive influences of transformational leadership (direct and indirect) and SEP practice on the coast tourism business’s performance.Practical implications Tourism business managers should pay attention to transformational leadership and SEP practice to lead to better performance for their businesses. Policymakers should educate businesses about new management practices to prepare for the changes in the future.Originality/value The findings of this study demonstrate that stakeholder theory can be used to explain the positive influences of transformational leadership and SEP practice on the coast tourism business’s performance in Thailand.
Disentangling the factors defining Bacillus subtilis group species abundance in natural soils
Authors: Xu Xinming , Pioppi Adele , Kiesewalter Heiko T. , Strube Mikael Lenz , Kovács Ákos T. 
Published: September 2024

AbstractBacillus subtilis is ubiquitously and broadly distributed in various environments but is mostly isolated from soil. Given that B. subtilis is known as a plant growth‐promoting rhizobacterium in agriculture, we aimed to describe the natural distribution of this species and uncover how biotic and abiotic factors affect its distribution. When comparing different soils, we discovered that B. subtilis group species are most abundant in grasslands but can rarely be isolated from forest soil, even if the soil sample sites are situated in proximity. Differential analysis revealed that spore‐forming bacteria exhibited enrichments in the grassland, suggesting niche overlap or synergistic interactions leading to the proliferation of certain Bacillus species in grassland environments. Network analysis further revealed that Bacillus and other Bacillota established a densely interconnected hub module in the grassland, characterised by positive associations indicating co‐occurrence, a pattern not observed in the forest soil. Speculating that this difference was driven by abiotic factors, we combined amplicon sequencing with physico‐chemical analysis of soil samples and found multiple chemical variables, mainly pH, to affect microbial composition. Our study pinpoints the factors that influence B. subtilis abundance in natural soils and, therefore, offers insights for designing B. subtilis‐based biocontrol products in agricultural settings.
The Faithless Analyst1
Authors: Stein Murray 
Published: September 2024

AbstractIn this lecture, I wish to speak about the faithless analyst. This is an analyst without fixed religious notions, without specific preferences with regard to religious faith and practice, without religious commitments or attachments of the kind that would influence the course of the analysis. Is this possible? Analysts like everyone else grow up in specific cultures that are deeply entangled with religious traditions. Can analysts shed this formation when they enter the consulting room? Should they? Values come with religious faith and practice. Should the analyst shed such religiously based values for the sake of absolute neutrality? I want to think about such faithlessness as an impossible ideal for analytical practice and how to work with failures to remain faithless in the presence of different faiths or the absence of faith among patients.