bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Sansure® Human Papillomavirus DNA Diagnostic Kit offers excellent reproducibility performance for the detection of high‐risk HPV
Authors: Prétet Jean‐Luc , Baraquin Alice , Chung Pui Yan Jenny , Puget Line , Dhillon Sharonjit K. , Tkachenka Yuliya , Jacquot Killian , Lepiller Quentin , Broeck Davy Vanden , Arbyn Marc 
Published: October 2024

AbstractCervical cancer screening is a cornerstone of cervical cancer elimination. Detection of high‐risk human papillomavirus (hrHPV) is recommended as the first step in screening provided that the assay used has been adequately validated. The Sansure® Human Papillomavirus DNA Diagnostic Kit is a new assay designed to detect HPV16, HPV18 and 13 other HPV in aggregate. The study aimed to evaluate the intra‐ and interlaboratory reproducibility of the assay according to international guidelines. Five hundred and fifty cervical residual cell samples from women attending cervical cancer screening were selected from the biobank of the HPV National Reference Centre in Belgium and used in this study. After DNA extraction, HPV was tested using the Sansure® Human Papillomavirus DNA Diagnostic Kit. The lower 95% confidence limit around the general reproducibility of this assay should be greater than or equal to 87%, with κ ≥ 0.50. Five hundred and thirty‐three samples had valid results. The Sansure® Human Papillomavirus DNA Diagnostic Kit demonstrated an excellent intra‐laboratory reproducibility of 93.8% (95% confidence interval [CI]: 91.4–95.7, κ = 0.85). The interlaboratory reproducibility was 93.4 (95% CI: 91.0–95.4, κ = 0.84). Intra and interlaboratory reproducibility were also excellent at the genotype level. Excluding HPV53 single infection samples from the analyses also resulted in excellent agreement. These data show that the Sansure® Human Papillomavirus DNA Diagnostic Kit is highly reproducible.
Macrophages can transmit coxsackievirus B4 to pancreatic cells and can impair these cells
Authors: Vergez Inès , Nekoua Magloire Pandoua , Arbrandt Gustav , Westman Jacob , Alidjinou Enagnon Kazali , Hober Didier 
Published: October 2024

AbstractMacrophages are suspected to be involved in the pathogenesis of type 1 diabetes. The role of macrophages in the transmission of coxsackievirus B4 (CVB4) to pancreatic cells and in the alteration of these cells was investigated. Human monocytes isolated from peripheral blood were differentiated into macrophages with M‐CSF (M‐CSF macrophages) or GM‐CSF (GM‐CSF macrophages). M‐CSF macrophages were inoculated with CVB4. M‐CSF and GM‐CSF macrophages were activated with lipopolysaccharide and interferon (IFN)‐γ. Human pancreatic beta cells 1.1B4 were inoculated with CVB4 derived from M‐CSF macrophages or were cocultured with CVB4‐infected M‐CSF macrophages. The antiviral activity of synthetic molecules in macrophage cultures was evaluated. Activated macrophages were cocultured with CVB4‐persistently infected 1.1B4 cells, and the specific lysis of these cells was determined. Our study shows that CVB4 can infect M‐CSF macrophages, leading to the release of interleukin‐6 and tumor necrosis factor‐α and later IFN‐α. M‐CSF macrophage‐derived CVB4 can infect 1.1B4 cells, which were then altered; however, when these cells were cultured in medium containing agarose, cell layers were not altered. Fluoxetine and CUR‐N373 can inhibit CVB4 replication in macrophage cultures. Supernatants of activated M‐CSF and GM‐CSF macrophage cultures induced lysis of CVB4‐persistently infected 1.1B4 cells. The cytolytic activity of activated GM‐CSF macrophages was higher towards CVB4‐persistently infected 1.1B4 cells than mock‐infected 1.1B4 cells. In conclusion, macrophages may play a role in CVB4 infection of pancreatic cells, and are capable of inducing lysis of infected pancreatic cells.
Male and female human papilloma virus infection and assisted reproductive technology outcomes: A comprehensive assessment from prevalence in semen to obstetric outcomes
Authors: Carullo Giorgia , Uceda Renteria Sara , Basili Ludovica , Marinello Davide , Di Stefano Giorgia , Mondini Irene , Casalechi Maíra , Volpi Mattia , Noli Stefania , Valzano Antonia , Callegaro Annapaola , Vercellini Paolo , Somigliana Edgardo , Reschini Marco , Viganò Paola 
Published: October 2024

AbstractInfertility, affecting approximately 16% of the global population, has led to increased reliance on reproductive medicine. The impact of human papillomavirus (HPV) infection in one or both partners on outcomes of Assisted Reproduction Technologies (ART) remains unclear. This prospective cohort study aimed to evaluate prevalence and effects of HPV infection in subjects and couples candidates to ART. A total of n = 510 men and n = 246 women were included and n = 145 couples (n = 290 individuals) had both partners enrolled in the study. The HPV semen infection rate was 17% (95% CI: 14–20) with HPV‐42, HPV‐16, HPV‐53 and HPV‐51 as the most frequently detected genotypes. In women, 26% (95% CI: 21–32) tested HPV‐positive in cervical swabs. In 6% (95% CI: 3–11) of the couples, both partners were positive but only three couples shared the same genotypes (HPV‐16; HPV‐39, HPV‐51, and HPV‐42; HPV‐31). Follicular fluids were positive in 20% (95% CI: 11–33) of samples, showing genotype discrepancies with cervical tests. Semen treatment could not completely eliminate the virus in positive samples but reduced the positivity to one‐third. No significant differences in semen and embryological variables, clinical pregnancy and live birth rates, neonatal and obstetrics outcomes were observed in subjects with positivity in semen or cervix compared to respective negative groups. Cumulative live birth rates per oocyte retrieval in couples where both partners were negative or both were positive did not differ, being 37% (95% CI: 28%–47%) and 44% (95% CI: 19–73), respectively. In conclusion, HPV testing should not be considered a prerequisite for accessing ART treatments. Robust inferences for natural fertility cannot be made using our findings, as the ART setting does not fully reflect natural conditions.
The bright and dark side of metaverse marketing
Authors: Lim Weng Marc , Bansal Sanchita , Nangia Priya , Singh Shifali 
Published: October 2024

AbstractThe metaverse is emerging from the amalgamation of augmented and virtual reality. Given the novelty and recency of the metaverse and its potential implications for marketing, this study aims to (i) explore how marketing could manifest and evolve in the metaverse and how consumer behavior may be influenced (what) by (ii) engaging in a systematic literature review focusing on the positives and negatives of the metaverse (how), so that (iii) marketers can plan for, progress in, and prosper from informed metaverse marketing strategies (so what). The study sheds light on the metaverse and its impact on marketing through an integrative framework that highlights the bright and dark side of the metaverse based on six key themes: increased digital innovation and transformation, new marketing possibilities, consumers’ interest in the metaverse, threatened digital privacy and legal challenges, health concerns, and sustainability issues. The study also presents 10 prominent cases of metaverse marketing in the real world to enrich and support the findings. The study concludes by outlining the directions for the future of metaverse marketing.
Growth performance, carcass characteristics and meat quality of Northern Finncattle steers compared to Holstein steers offered grass silage‐grain‐based ration
Authors: Keto Liisa , Manni Katariina , Tuomivaara Anne , Soppela Päivi , Huuskonen Arto 
Published: October 2024

AbstractBackgroundFinnish beef production is largely based on dairy breeds. Eastern Finncattle, Western Finncattle, and Northern Finncattle (NFC) are native breeds, whereas Holstein (HO) and Nordic Red are the most important commercial dairy breeds. The NFC has been the closest to extinction among Finnish native cattle breeds. The objective of the experiment was to provide data on meat quality of NFC compared to HO. To our knowledge no previously published scientific data about the meat quality traits of NFC is available.ResultsThe targeted slaughter age was 600 days for both NFC and HO. The carcass gain of the HO steers was 60% higher compared to NFC steers. No breed differences in dressing proportion, carcass conformation, or carcass fat score were observed. Breed had no effect on the pH, color, drip loss, chemical composition, shear force value, or sensory tenderness of the longissimus lumborum muscle. However, beef flavor of the NFC steers was evaluated 7% better compared to the HO steers. In addition, the longissimus lumborum muscle of the NFC steers tended to be juicier than that of the HO steers.ConclusionsOnly little variability in meat quality traits was observed between the breeds. However, NFC requires a longer growing period than HO when targeting the same carcass weight. To promote the use of the NFC in beef production, it is necessary to find other than economic production efficiency features, which would provide added value for premium meat quality to compensate the lower production efficiency.
Macrokinetics of ammonium sulfite oxidation inhibited by sodium thiosulfate in wet ammonium flue gas desulfurization
Authors: Peng Jian , Yao Wen , Lian Peichao 
Published: October 2024

AbstractThe oxidation of ammonium sulfite must be inhibited to improve the economy and cycle performance of the ammonia flue gas desulfurization process. A stirred bubbling reactor was used to investigate the macrokinetics of oxidation inhibition of ammonium sulfite by sodium thiosulfate in wet ammonia desulfurization. The effects of initial concentration, Na2S2O3 concentration, pH value, gas flow rate, and temperature on the oxidation rate were discussed. The results show that the apparent activation energy of the reaction is 37.3 kJ/mol. The macrokinetics equation of sodium thiosulfate inhibiting sulfite oxidation in the ammonia desulfurization process was established. Combined with the kinetic model, it is inferred that the rate of sodium thiosulfate inhibiting ammonium sulfite oxidation is controlled by the intrinsic chemical reaction. The results can provide a reference for the regeneration of ammonium sulfite in the wet ammonia desulfurization process.
Outcomes of Surgical Repair of Adult Naso‐Orbital‐Ethmoid Fractures: A Systematic Review and Meta‐Analysis
Authors: Conti Keith R. , Bhat Akash M. , Nguyen Shaun A. , Rohloff Rhonda , Keeler Jarrod A. 
Published: October 2024

ObjectivesAdult naso‐orbital‐ethmoid (NOE) fractures are estimated to account for 5% of all adult facial fractures without published consensus on management. The purpose of this investigation was to assess the available literature regarding the treatment and outcomes of adult naso‐orbital‐ethmoid fractures.Data SourcesCochrane Library, PubMed, Scopus, and CINAHL.MethodsFollowing PRISMA guidelines, databases were searched from inception through July 25, 2024 for studies pertaining to the treatment of NOE fractures. Measures of interest included patient demographics, associated fractures, type of intervention, and complications.ResultsA total of 16 studies were included for meta‐analysis, consisting of 459 patients. The patients included in the analysis had a mean age of 30.6 years (95% CI: 26.9–34.3 years) with a male‐to‐female gender ratio of 2.7:1. Operative intervention, specifically open reduction and internal fixation (ORIF) (90.1%; 95: CI: 76.6–98.1%), was the most commonly performed management. Closed reduction has been reported for all three types. The most frequently reported complications included nasolacrimal duct obstruction (38.6%; 95% CI: 10.6–71.7%), postoperative epiphora (24.9%; 95% CI: 6.4–50.4%), and telecanthus (20.9%; 95% CI: 1.7–53.5%).ConclusionsSurgical intervention can be considered for all NOE types. Despite surgical intervention, NOE fractures remain difficult to treat, and inadequate repair may result in complications. Laryngoscope, 135:991–999, 2025
Mid‐Term Effects of Postfire Mulching With Straw or Wood Chips on Soil Erosion in Semi‐Arid Forests
Authors: Plaza‐Álvarez Pedro Antonio , Lucas‐Borja Manuel Esteban , Carmona‐Yáñez María Dolores , Candel‐Pérez David , Zema Demetrio Antonio 
Published: October 2024

ABSTRACTSoil mulching has been studied as a postfire management action to reduce soil erosion in several forest environments. Less research exists about the effectiveness of mulching with straw and wood chips beyond the first year after a wildfire on sites with different slopes. To fill this gap, this study has measured soil erosion in three burned sites (untreated soils, and soils mulched using wheat straw or pine wood chips) throughout a 2.5‐year observation period in a forest of Castilla‐La Mancha (Central Eastern Spain). Soil condition and slope (gentle, < 32%, gentle vs. steep, > 38%, slopes) significantly influenced erosion, which, however, was of low entity due to the relatively low rainfall erosivity. Mulching was generally effective after the most intense events (maximum 30‐min rainfall intensity over 15 mm/h). On the gentle hillslopes, mulch application did not reduce postfire soil loss compared with the untreated sites. In contrast, on the steep slopes, the effectiveness of soil mulching was significant for the two most intense rainfall events (−30% of soil loss in plots treated with wheat straw compared with the burned and untreated sites). The cumulated soil loss significantly decreased on the treated sites (−40%) only when wheat straw was used. On steep slopes, the anti‐erosive effects of mulching were almost durable, since the mulch covered more than one‐third of the plot areas until the end of the monitoring period. These results help land managers adopt the most effective measures of postfire management in semi‐arid forests affected by severe wildfires.
Suppressed Ion Migration in Tin Halide Perovskites for Stable X‐Ray Detectors with Low Dark Current Drift
Authors: Wang Xu , Bian Yangshuang , Wang Hao , Wang Junfang , Liu Tianhua , Xiao Hongbin , Guo Yunlong , Wei Wenjuan , Yang Yang (Michael) , Meng Xiangyue 
Published: October 2024

AbstractIon migration is a major factor affecting the operational stability of perovskite X‐ray detectors, especially under the necessary high working bias. To address this issue, lead‐free tin halide perovskites (FASnI3) being pioneered with the robust Sn─I bond for X‐ray detection. It is observed that the incorporation of propylamine hydroiodide (PAI) to FASnI3‐PAI films can increase activation energy for ion migration (752.18 meV), thereby enhancing the operational stability of X‐ray detectors. The devices based on FASnI3‐PAI demonstrate a significant reduction in dark current drift (1.14 × 10−16 A cm−1 V−1 s−1), with values two orders of magnitude lower than those observed in lead halide perovskites (2.40 × 10−14 A cm−1 V−1 s−1). Moreover, the FASnI3‐PAI films exhibit enhanced film crystallinity, reduced trap density, and improved mobility‐lifetime product, resulting in a high sensitivity of 5535 µC Gyair−1 cm−2 and an ultralow detection limit of 7.95 nGyair s−1. Significantly, high‐contrast X‐ray imaging has also been successfully achieved.
Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering.
Authors: Rodrigues Francisco A. P. , Oliveira Cláudia S. , Sá Simone C. , Tavaria Freni K. , Lee Sang Jin , Oliveira Ana L. , Costa João B. 
Published: October 2024

AbstractSignificant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro‐ and anti‐angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.
CO2‐Responsive Copolymers for Membrane Applications, Synthesis, and Performance Evaluation
Authors: Pashayev Emil , Georgopanos Prokopios 
Published: October 2024

AbstractThe urgent need to mitigate climate change has spurred research into innovative carbon dioxide (CO2) capture materials. In this study, the design and synthesis of CO2‐responsive diblock copolymers, poly (N‐[3‐(dimethylamino)propyl]‐acrylamide)‐b‐poly(methyl methacrylate) (PDMAPAm‐b‐PMMA) are focused on via a two‐step reversible addition−fragmentation chain‐transfer (RAFT) polymerization as well as the application of the synthesized diblock copolymer as a membrane for CO2 capture. The resulting diblock copolymer possesses distinct blocks with varying properties. The poly (N‐[3‐(dimethylamino)propyl]‐acrylamide) (PDMAPAm) block provides CO2‐responsive behavior, while the poly(methyl methacrylate) (PMMA) block contributes to mechanical stability. The gas transport properties of the fabricated thin‐film composite membrane made of PDMAPAm‐b‐PMMA are measured. It is determined that the copolymer exhibits dual responsiveness towards CO2 and can be tailored for use in fabrication of membranes for direct air capture (DAC).
Thermal Conductivity of Polymers: A Simple Matter Where Complexity Matters
Authors: Mukherji Debashish 
Published: October 2024

AbstractThermal conductivity coefficient κ measures the ability of a material to conduct a heat current. In particular, κ is an important property that often dictates the usefulness of a material over a wide range of environmental conditions. For example, while a low κ is desirable for the thermoelectric applications, a large κ is needed when a material is used under the high temperature conditions. These materials range from common crystals to commodity amorphous polymers. The latter is of particular importance because of their use in designing light weight high performance functional materials. In this context, however, one of the major limitations of the amorphous polymers is their low κ, reaching a maximum value of ≈0.4 W/Km that is 2–3 orders of magnitude smaller than the standard crystals. Moreover, when energy is predominantly transferred through the bonded connections, κ ⩾ 100 W/Km. Recently, extensive efforts have been devoted to attain a tunability in κ via macromolecular engineering. In this work, an overview of the recent results on the κ behavior in polymers and polymeric solids is presented. In particular, computational and theoretical results are discussed within the context of complimentary experiments. Future directions are also highlighted.
Perforator‐to‐Perforator SCIP‐Based Vascularized Lymphnode Transfer to Reduce Morbidity and Increase Efficacy in Lymphedema Surgery: Preliminary Results With 12 Cases
Authors: Meroni Matteo , Scaglioni Mario F. 
Published: October 2024

ABSTRACTBackgroundVascularized lymphnode transfer (VLNT) is one of the most common surgical treatments for moderate and severe lymphedema. Various techniques have been described for harvesting lymph nodes from different donor sites. However, a standardized harvest procedure is still lacking. The transplantation of inguinal lymph nodes using the perforator‐to‐perforator technique may represent a significant advancement in this context. This approach relies always on the same vascular pedicle, offers a lower morbidity, and allows for a more superficial inset at the recipient site.Patients and MethodsBetween 2019 and 2022, 12 patients received a perforator‐to‐perforator VLNT for the treatment of late stage (Late II) lymphedema, both primary and secondary. In all cases, the lymphnodes were harvested from the groin supplied by the superficial circumflex iliac artery perforator (SCIP) vessels. The average age was 62.2 years old (range 47–73 years old); nine patients were females and three were males. In 11 cases, the lower limb was affected, and in one case, the upper limb was affected. Eight patients received additional lymphovenous anastomosis.ResultsAll the patients reported an improvement of the symptoms after surgery in terms of tissue quality (tenderness, lymphangiectasia, and pain), and no cases of recurrent cellulitis were reported. Postoperative lymphoscintigraphy was performed at 6 months, and in all cases, the function of transplanted lymphnodes was confirmed. No complications were encountered, neither at donor nor at recipient site. The follow‐up was at least 12 months in all patients.ConclusionsDespite being more technically demanding, the systematic implementation of the perforator‐to‐perforator technique for the transfer of SCIP‐based inguinal lymphnodes can be a valuable evolution of VLNT. The aim of this study is to present how this technical approach may become a standardized procedure for inguinal‐based VLNT, offering a reduced donor and recipient site morbidity and potentially enhancing the lymph draining effects due to a more superficial inset.
GCLmf: A Novel Molecular Graph Contrastive Learning Framework Based on Hard Negatives and Application in Toxicity Prediction**
Authors: Yu Xinxin , Chen Yuanting , Chen Long , Li Weihua , Wang Yuhao , Tang Yun , Liu Guixia 
Published: October 2024

AbstractIn silico methods for prediction of chemical toxicity can decrease the cost and increase the efficiency in the early stage of drug discovery. However, due to low accessibility of sufficient and reliable toxicity data, constructing robust and accurate prediction models is challenging. Contrastive learning, a type of self‐supervised learning, leverages large unlabeled data to obtain more expressive molecular representations, which can boost the prediction performance on downstream tasks. While molecular graph contrastive learning has gathered growing attentions, current models neglect the quality of negative data set. Here, we proposed a self‐supervised pretraining deep learning framework named GCLmf. We first utilized molecular fragments that meet specific conditions as hard negative samples to boost the quality of the negative set and thus increase the difficulty of the proxy tasks during pre‐training to learn informative representations. GCLmf has shown excellent predictive power on various molecular property benchmarks and demonstrates high performance in 33 toxicity tasks in comparison with multiple baselines. In addition, we further investigated the necessity of introducing hard negatives in model building and the impact of the proportion of hard negatives on the model.