bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ca2+-ATPase and function of sarcoplasmic reticulum during cardiac hypertrophy
Authors: Levitsky Dmitri , Bastie Diane De La , Schwartz Ketty , Lompré Anne-Marie 
Published: December 2017

The properties of the calcium pump system of sarcoplasmic reticulum (SR) were studied in a series of 34 rats subjected to cardiac overload and 19 sham-operated animals. Total homogenates of left ventricle were analyzed by measuring the oxalate-supported Ca2+ uptake rate, the steady-state level of the phosphorylated intermediate of Ca2+-adenosine triphosphatase (Ca2+-ATPase) (E-P), and the amount of Ca2+-ATPase mRNA. All three parameters decreased gradually as a function of the relative left ventricular weight increase. The calcium-sensitivity curves showed that the velocity of Ca2+ transport in SR from the hypertrophied heart is diminished at low as well as optimal Ca2+ concentrations, with the dissociation constant (Kd) value for Ca2+ unchanged from that of the control preparation. Taken together with the results presented in our recent publication (De la Bastie, Levitsky, Mercadier, Marotte, Wisnewsky, Brovkovivh, Schwartz, and Lompré, Circ. Res. 66: 554–564, 1990), these data strongly indicate that differences in the Ca2+ pump activities of SR from normal and hypertrophied rat hearts are due to quantitative rather than qualitative changes of the Ca2+-ATPase protein. calcium ion uptake; calcium ion adenosine triphosphatase messenger ribonucleic acid; cardiac hypertrophy; monoclonal antibody
Thyroid control over membrane processes in rat heart
Authors: Seppet Enn K. , Kadaya Lumme Y. , Hata Tomoji , Kallikorm Ants P. , Saks Valdur A. , Vetter Roland , Dhalla Naranjan S. 
Published: December 2017

We have studied the effects of hypo- and hyperthyroidism on sarcolemmal (SL) and sarcoplasmic reticular (SR) ion transport processes and mitochondrial energy production in rat heart. The following conclusions were derived. 1) Compared with euthyroid state, hyperthyroidism led to increased SR Ca2+-accumulation. In SL, the activities of Ca2+-stimulated adenosine triphosphatase (ATPase), ATP-dependent Ca2+ pumping, and Na+-Ca2+ exchanger were not affected; but ouabain-sensitive Na+-K+-ATPase activity was enhanced. 2) Hypothyroidism resulted in depressed activities of Ca2+ pumps both in SL and SR. In SL, the Na+-K+-ATPase activity was decreased, but Na+-Ca2+ exchange was unaltered. 3) Thus slower relaxation of the hypothyroid myocardium may be attributed to depressed functioning of Ca2+ pumps in SR and SL, whereas faster relaxation of the hyperthyroid heart may be based on increased Ca2+-pumping activity of SR. 4) Hyperthyroidism and hypothyroidism, respectively, led to enhanced and decreased rates of mitochondrial phosphocreatine synthesis. The thyroid state appears to control the functional coupling between mitochondrial creatine kinase and ATP-ADP translocase: the energy of oxidative phosphorylation was transformed into phosphocreatine more effectively in mitochondria from hypothyroid hearts than in those from hyperthyroid hearts. euthyroid; hyperthyroid; hypothyroid; mitochondrial creatine kinase; myocardium; oxidative phosphorylation; phosphocreatine synthesis; sarcolemma; sarcoplasmic reticulum
Na+-K+-ATPase-G protein coupling in myocardial sarcolemma: separation and reconstitution
Authors: Danilenko Mikhail P. , Turmukhambetova Vera C. , Yesirev Oleg V. , Tkachuk Vsevolod A. , Panchenko Mikhail P. 
Published: December 2017

The cholinergic agonist carbachol produces a concentration-dependent (half-maximum inhibitory concentration = 0.9 μM) decrease in the Na+-K+-adenosine triphosphatase (ATPase) activity of rabbit cardiac sarcolemma that occurred only in the presence of guanosine 5'-[-thio]triphosphate (0.1 μM GTPS) and reached 40% inhibition. The inhibition is blocked by the muscarinic receptor antagonist atropine (10 μM) and is abolished in sarcolemma treated with pertussis toxin (20 μg/ml) in the presence of 100 μM NAD. GTPS alone reduces Na+–K+-ATPaseactivity by 45% (half-maximum inhibitory = 1 μM). The apparent affinity of the enzyme for GTPS is increased 10-fold in the presence of 1 μM carbachol. In sarcolemma solubilized with the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS, 10 mM), the GTPS-dependent inhibition of the Na+-K+-ATPase is also observed. Gel filtration of a CHAPS extract of sarcolemma on a Sepharose CL–6B column resulted in a separation of Na+-K+-ATPase and pertussis toxin-sensitive Gi activities. Na+-K+-ATPase activity that was separated on the column lost its sensitivity to the inhibitory action of guanine nucleotides. Inhibitory effects (20–30%) of guanosine 5'-triphosphate analogues [Gpp(NH)p, GTPS, or Gpp(CH2)p] at micromolar concentrations were restored when the Na+-K+-ATPase activity was recombined with fractions that contained the pertussis toxin-sensitive Gi protein(s). Similar concentrations of guanosine 5'-triphosphate, guanosine 5'-diphosphate, guanosine-5'-[β-thio]diphosphate, or App(NH)p were unable to induce the Gi protein-mediated attenuation of Na+-K+-ATPase activity in the reconstitution system. These results suggest that a pertussis toxin-sensitive Gi protein may act as a transducer of the inhibitory hormonal signals on Na+-K+-ATPase in the sarcolemma. cardiac sarcolemma
Intracavitary ultrasound impairs left ventricular performance: presumed role of endocardial endothelium
Authors: Gillebert Thierry C. , De Hert Stefan G. , Andries Luc J. , Jageneau Anton H. , Brutsaert Dirk L. 
Published: December 2017

Irradiation of isolated cardiac muscle by high-power, high-frequency, continuous wave ultrasound selectively damages endocardial endothelium (EE). We evaluated this ultrasound effect in vivo on the performance of the intact ejecting canine left ventricle (LV). A cylindrical ultrasound probe (0.9 MHz, 25 W), mounted on a catheter, was inserted in the LV cavity through an apical stab wound and was activated for 60, 120, and 240 s, followed each time by a recovery period of 10–15 min. Ultrasound transiently and repeatedly abbreviated the time interval from end diastole to peak (-)dP/dt (from 241 ± 30 to 229 ± 32 ms after 240 s; P < 0.001), accelerated LV pressure fall, did not alter peak (+)-dP/dt or peak systolic pressure, increased diastolic and systolic segment lengths, and decreased fractional shortening. Microscopic analysis revealed dispersed granulocytes attached to the EE. EE cells were visibly damaged only in a limited area surrounding the probe. Accordingly, high-power, high-frequency, continuous wave ultrasound reversibly modulated LV performance, presumably by transient alteration of EE function. cardiac function; contractility; diastole; relaxation Submitted on May 7, 1991 Accepted on April 28, 1992
Acute compensation to abrupt occlusion of rat femoral artery is prevented by NO synthase inhibitors
Authors: Unthank J. L. , Nixon J. C. , Dalsing M. C. 
Published: December 2017

Pages H5237–H2530: J. L. Unthank, J. C. Nixon, and M. C. Dalsing. “Acute compensation to abrupt occlusion of rat femoral artery is prevented by NO synthase inhibitors.” The volume number and year in the abstract line were incorrectly cited and should appear as the following. Unthank, Joseph L., J. Craig Nixon, and Michael C. Dalsing. Acute compensation to abrupt occlusion of rat femoral artery is prevented by NO synthase inhibitors. Am. J. Physiol. 267 ( Heart Circ. Physiol. 36): H2523–H2530, 1994.
Influence of the Thyroid Gland on Jejunal Secretion in the Dog
Authors: Russell Raymond A. , Nasset E. S. 
Published: December 2017

Page 103: Raymond A. Russell and E. S. Nasset, "Influence of the Thyroid Gland on Secretion in the Dog." In table 1, under Dog 2, the last line should be as follows: (See PDF)
Rate and affinity in human red blood cell sugar transport
Authors: LeFevre Paul G. 
Published: December 2017

Page 286: LeFevre, Paul G., "Rate and affinity in human red blood cell sugar transport." The legend for Fig. 2 should read: Uncertainty in constants as estimated from half-times in Fig. 1. The four curves define the relations between k and K of the type given in equation 4,.... (See PDF for Equation)
The size principle: a rule describing the recruitment of motoneurons
Authors: Mendell Lorne M. 
Published: December 2017

This essay looks at the historical significance of five APS classic papers that are freely available online: McPhedran AM, Wuerker RB, and Henneman E. Properties of motor units in a homogeneous red muscle (soleus) of the cat. J Neurophysiol 28: 71–84, 1965 ( http://jn.physiology.org/cgi/reprint/28/1/71 ). Wuerker RB, McPhedran AM, and Henneman E. Properties of motor units in a heterogeneous pale muscle (m. gastrocnemius) of the cat. J Neurophysiol 28: 85–99, 1965 ( http://jn.physiology.org/cgi/reprint/28/1/85 ). Henneman E, Somjen G, and Carpenter DO. Functional significance of cell size in spinal motoneurons. J Neurophysiol 28: 560–580, 1965 ( http://jn.physiology.org/cgi/reprint/28/3/560 ). Henneman E and Olson CB. Relations between structure and function in the design of skeletal muscles. J Neurophysiol 28: 581–598, 1965 ( http://jn.physiology.org/cgi/reprint/28/3/581 ). Henneman E, Somjen G, and Carpenter DO. Excitability and inhibitability of motoneurons of different sizes. J Neurophysiol 28: 599–620, 1965 ( http://jn.physiology.org/cgi/reprint/28/3/599 ).
Bronchial hyperreactivity occurs in steroid-treated guinea pigs depleted of leukocytes by cyclophosphamide
Authors: Murlas C. , Roum J. H. 
Published: December 2017

Page 1630: C, Murlas and J. H. Roum, “Bronchial hyperreactivity occurs in steroid-treated guinea pigs depleted of leukocytes by cyclophosphamide.” Page 1630: the first sentence of methods should read: Forty-seven male Hartley strain guinea pigs (550–750 g in weight) were used in the study. Page 1631, column 1, paragraph 1: next to last sentence should read: The remaining animals were divided into groups of four or more. Page 1634: Figs. 4B and 5B are derived from Ref. 21. Page 1635: Fig. 7 legend, fourth and fifth sentences should read: Values represent means ± SE for 4 animals at each time point except at 2 h for normal animals ( n = 7). Values for normal animals are indicated by open bars and are derived from Ref. 21. Page 1633, corrected Fig. 20 should be substituted: (See PDF)
Bath rewarming from immersion hypothermia
Authors: Hoskin R. W. , Melinyshyn M. J. , Romet T. T. , Goode R. C. 
Published: December 2017

Page 1518: R. W. Hoskin, M. J. Melinyshyn, T. T. Romet, and R. C. Goode. “Bath rewarming from immersion hypothermia.” Page 1518: the 14th through the 16th lines of the abstract should read: It was concluded that trunk-only rewarming is not superior to whole-body bath rewarming as a therapy for mild immersion hypothermia.... Page 1521: the seventh and eighth lines of the last paragraph of discussion should read: Thus, it could be argued that the real physiological afterdrop was the difference....
Changes in breath 13CO2/12CO2 consequent to exercise and hypoxia
Authors: Barstow T. J. , Cooper D. M. , Epstein S. , Wasserman K. 
Published: December 2017

Page 936: T. J. Barstow, D. M. Cooper, S. Epstein, and K. Wasserman. “Changes in breath 13CO2/12CO2, consequent to exercise and hypoxia.” Page 936, right column, sentence beginning on line 8 should read: We therefore wondered if changes in EN could be used to detect changes in endogenous substrate oxidation during exercise. Page 940, right column, sentence beginning on line 15 should read: We controlled for potential acute modulators of oxidative fuel mix (e.g., meal and activity) by the overnight fast and avoidance of strenuous activity 24 h before each test. Page 941, left column, sentence beginning on line 27 should read: In summary, Vo2, adjusts slightly more rapidly than Vo2 in the transition from rest to exercise, whereas 13CO2 adjusts much more slowly. Page 938, Table 3 footnote should read: Values are group means for individual responses averaged from 5 to 20 min of exercise. n = 7 except for WR-3 where n = 6. Hypoxia represented FiO02 = 0.15, room air Fioo2 = 0.207. * Significantly different from both WR-1 conditions and WR-2 in room air (P < 0.05). Note that work above LT (WR-2 in hypoxia and WR-3) resulted in a significant increase in R.
ImmunoMap: A Bioinformatics Tool for T-cell Repertoire Analysis
Authors: Sidhom John-William , Bessell Catherine A. , Havel Jonathan J. , Kosmides Alyssa , Chan Timothy A. , Schneck Jonathan P. 
Published: December 2017

Abstract
Despite a dramatic increase in T-cell receptor (TCR) sequencing, few approaches biologically parse the data in a fashion that both helps yield new information about immune responses and may guide immunotherapeutic interventions. To address this issue, we developed a method, ImmunoMap, that utilizes a sequence analysis approach inspired by phylogenetics to examine TCR repertoire relatedness. ImmunoMap analysis of the CD8 T-cell response to self-antigen (Kb-TRP2) or to a model foreign antigen (Kb-SIY) in naïve and tumor-bearing B6 mice showed differences in the T-cell repertoire of self- versus foreign antigen-specific responses, potentially reflecting immune pressure by the tumor, and also detected lymphoid organ–specific differences in TCR repertoires. When ImmunoMap was used to analyze clinical trial data of tumor-infiltrating lymphocytes from patients being treated with anti–PD-1, ImmunoMap, but not standard TCR sequence analyses, revealed a clinically predicative signature in pre- and posttherapy samples. Cancer Immunol Res; 6(2); 151–62. ©2017 AACR.
A EPOPEIA INDIANA N’O REI DE BENARES DE FERNANDO LEAL (1846-1910)
Authors: Silva Regina Célia Pereira da 
Published: December 2017

Mais conhecido pelas suas traduções feitas do francês do que pelas suas obras literárias, Fernando Leal é um escritor que vale a pena estudar e desvendar não só pelo seu estilo linguístico peculiar mas também pela constante relação dialógica existente entre duas culturas no íntimo do eu-literário. Este estudo focaliza-se, de modo especial, na análise do poema O Rei de Benares, escrito na cidade de Lisboa e destinado a um público formado por crianças. Influenciado pela antiga epopeia indiana, o poema apresenta novos elementos constituitivos da lírica e da poética provenientes de uma convivência constante de duas culturas diferentes, a portuguesa e a indiana, num mesmo indivíduo. Através da análise e comparação profunda dos textos poéticos quer-se penetrar no sentido último do poema e contribuir para uma nova compreensão sócio-cultural do homem, não só como membro de uma comunidade multicultural mas também como ser dialógico.
From the “Closed” to the “Open” Commercial State: A Very Brief History of International Economic Law
Authors: Schütze Robert 
Published: December 2017

Abstract
Commerce had become a “reason of state” in the eighteenth century. With the publication of Smith’s Wealth of Nations, the idea that free international trade was economically beneficial however gradually gained prominence. The long nineteenth century indeed saw timid – bilateral – attempts to implement the new politico-economic philosophy. Yet the First World War and the Great Depression destroyed almost all trade liberalisation already achieved, and the neo-mercantilist policies of the 1920s and 1930s seemed to turn the wheels of commerce backwards. Nonetheless: after the Second World War the belief that a peaceful world order could only be founded on a stable international economic order emerged strengthened; and a number of institutional attempts to liberalise – and harness – the world economy were immediately made, culminating in the creation of the 1947 General Agreement on Tariffs and Trade.
Evolution, trends, outcomes, and economics of hematopoietic stem cell transplantation in severe autoimmune diseases
Authors: Snowden John A. , Badoglio Manuela , Labopin Myriam , Giebel Sebastian , McGrath Eoin , Marjanovic Zora , Burman Joachim , Moore John , Rovira Montserrat , Wulffraat Nico M. , Kazmi Majid , Greco Raffaella , Snarski Emilian , Kozak Tomas , Kirgizov Kirill , Alexander Tobias , Bader Peter , Saccardi Riccardo , Farge Dominique 
Published: December 2017

Key Points
Using EBMT data in >2000 patients from 1994 to 2015, we analyzed the development and factors influencing outcomes of HSCT for ADs. Transplant center experience, accreditation, and national socioeconomic factors are relevant for outcomes and future health service delivery.
Complete genome sequence of esterase-producing bacterium Croceicoccus marinus E4A9T
Authors: Wu Yue-Hong , Cheng Hong , Huo Ying-Yi , Xu Lin , Liu Qian , Wang Chun-Sheng , Xu Xue-Wei 
Published: December 2017

AbstractCroceicoccus marinusE4A9Twas isolated from deep-sea sediment collected from the East Pacific polymetallic nodule area. The strain is able to produce esterase, which is widely used in the food, perfume, cosmetic, chemical, agricultural and pharmaceutical industries. Here we describe the characteristics of strain E4A9, including the genome sequence and annotation, presence of esterases, and metabolic pathways of the organism. The genome of strain E4A9Tcomprises 4,109,188 bp, with one chromosome (3,001,363 bp) and two large circular plasmids (761,621 bp and 346,204 bp, respectively). Complete genome contains 3653 coding sequences, 48 tRNAs, two operons of 16S–23S-5S rRNA gene and three ncRNAs. Strain E4A9Tencodes 10 genes related to esterase, and three of the esterases (E3, E6 and E10) was successfully cloned and expressed inEscherichia coliRosetta in a soluble form, revealing its potential application in biotechnological industry. Moreover, the genome provides clues of metabolic pathways of strain E4A9T, reflecting its adaptations to the ambient environment. The genome sequence ofC. marinusE4A9Tnow provides the fundamental information for future studies.
Whole exome sequencing identifies a novel homozygous frameshift mutation in the ASPM gene, which causes microcephaly 5, primary, autosomal recessive
Authors: Bhargav Desaraju Suresh , Sreedevi N. , Swapna N. , Vivek Soumya , Kovvali Srinivas 
Published: December 2017

Microcephaly is a genetically heterogeneous disorder and is one of the frequently notable conditions in paediatric neuropathology which exists either as a single entity or in association with other co-morbidities. More than a single gene is implicated in true microcephaly and the list is growing with the recent advancements in sequencing technologies. Using massive parallel sequencing, we identified a novel frame shift insertion in the abnormal spindle-like microcephaly-associated protein gene in a client with true autosomal recessive primary microcephaly. Exome sequencing in the present case helped in identifying the true cause behind the disease, which helps in the premarital counselling for the sibling to avoid future recurrence of the disorder in the family.
Tuition reduction is the key factor determining tax burden of graduate students under the Tax Cuts and Job Act
Authors: Lawston Patricia M. , Parker Michael T. 
Published: December 2017

Background: The proposed Tax Cuts and Jobs Act (H.R.1) has stirred significant public debate on the future of American economics. While supporters of the plan have championed it as a necessity for economic revitalization, detractors have pointed out areas of serious concern, particularly for low- and middle-income Americans. One particularly alarming facet of the plan is the radical change to education finance programs and taxation of students in higher education. Methods: By analyzing actual income and tuition of a public and a private university student, as well as the ‘average’ graduate student, we investigated the effect of both the House and Senate versions of H.R. 1 on taxation of students of various family structures. Results: Our findings indicate that taxable tuition would be the greatest contributor to graduate student tax burden across all four categories of filing status. However, when tuition reduction is upheld or a student is on sustaining fees rather than full tuition, graduate students would realize decreases in taxation. Conclusions: Overall, we conclude that removal of tuition reduction would result in enormous tax burdens for graduate students and their families and that these effects are dependent not only on the status of the student in their degree program but also on their tuition and stipend, and therefore the institution they attend.
Effect of target color and scanning geometry on terrestrial LiDAR point-cloud noise and plane fitting
Authors: Bolkas Dimitrios , Martinez Aaron 
Published: December 2017

AbstractPoint-cloud coordinate information derived from terrestrial Light Detection And Ranging (LiDAR) is important for several applications in surveying and civil engineering. Plane fitting and segmentation of target-surfaces is an important step in several applications such as in the monitoring of structures. Reliable parametric modeling and segmentation relies on the underlying quality of the point-cloud. Therefore, understanding how point-cloud errors affect fitting of planes and segmentation is important. Point-cloud intensity, which accompanies the point-cloud data, often goes hand-in-hand with point-cloud noise. This study uses industrial particle boards painted with eight different colors (black, white, grey, red, green, blue, brown, and yellow) and two different sheens (flat and semi-gloss) to explore how noise and plane residuals vary with scanning geometry (i.e., distance and incidence angle) and target-color. Results show that darker colors, such as black and brown, can produce point clouds that are several times noisier than bright targets, such as white. In addition, semi-gloss targets manage to reduce noise in dark targets by about 2–3 times. The study of plane residuals with scanning geometry reveals that, in many of the cases tested, residuals decrease with increasing incidence angles, which can assist in understanding the distribution of plane residuals in a dataset. Finally, a scheme is developed to derive survey guidelines based on the data collected in this experiment. Three examples demonstrate that users should consider instrument specification, required precision of plane residuals, required point-spacing, target-color, and target-sheen, when selecting scanning locations. Outcomes of this study can aid users to select appropriate instrumentation and improve planning of terrestrial LiDAR data-acquisition.
Preparation of poly(L-lactide)/poly(ethylene glycol)/organo-modified montmorillonite nanocomposites via melt intercalation under continuous elongation flow
Authors: Wu Ting , Yuan Ding , Qu Jin-Ping 
Published: December 2017

Abstract
An innovative eccentric rotor extruder (ERE), which can generate continuous elongation flow, was used to prepare the poly(L-lactide) (PLLA)/poly(ethylene glycol) (PEG)/organo-modified montmorillonite (OMMT) nanocomposites. The morphology was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM), and showed that the OMMT nanoparticles were uniformly dispersed in the matrix and mainly existed in intercalation mode. The influence of OMMT on the rheological behavior of plasticized PLLA was investigated by dynamic rheological measurements, showing greater improvement of rheological properties compared to that of PLLA/PEG blend. The crystallization behavior and crystalline structures were studied by differential scanning calorimetry (DSC) and XRD, respectively, and showed that the presence of OMMT further speeds up the crystallization rate of plasticized PLLA. However, the crystallization rate and crystallinity had a slight downward trend at high OMMT content because of the inhibition effect of the OMMT percolation network structure. Polarizing optical microscopy (POM) was further carried out and proved that the OMMT nanoparticles as a heterogeneous nucleating agent can increase the spherulite growth rate and nucleation density. The thermal stability was investigated by thermogravimetric analysis and indicated that the addition of OMMT at low concentration can improve the thermal stability of plasticized PLLA more effectively.