bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of self‐setting β‐tricalcium phosphate granular cement
Authors: Fukuda Naoyuki , Tsuru Kanji , Mori Yoshihide , Ishikawa Kunio 
Published: April 2017

AbstractBone defect reconstruction would be greatly improved if β‐tricalcium phosphate (β‐TCP) granules had the ability to self‐set without sacrificing their osteoconductivity potential. This study aimed to identify a method to permit β‐TCP self‐setting whilst maintaining good osteoconductivity. When mixed with acidic calcium phosphate solution, β‐TCP granules were found to readily set, forming a fully interconnected porous structure. On mixing, dicalcium phosphate dihydrate crystals formed on the surface of β‐TCP granules, bridging the granules and resulting in the setting reaction. The setting time of the β‐TCP granular cement (β‐TCP GC) was approximately 1 min and its mechanical strength, in terms of diametral tensile strength, was approximately 0.8 MPa. The β‐TCP GC and β‐TCP granules both showed the same level of osteoconductivity within rat calvaria bone defects. At 2 and 4 weeks post‐implantation, new bone formation was comparable between the two β‐TCP based bone substitutes. We conclude that β‐TCP GC has excellent potential for use as a cement in bone defect reconstruction. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 800–807, 2018.
The Ginsenoside Derivative 20(S)‐Protopanaxadiol Inhibits Solar Ultraviolet Light‐Induced Matrix Metalloproteinase‐1 Expression
Authors: Han Seungmin , Lim Tae‐Gyu , Kim Jong‐Eun , Yang Hee , Oh Deok‐Kun , Yoon Park Jung Han , Kim Hee Jung , Rhee Young Kyoung , Lee Ki Won 
Published: April 2017

ABSTRACTGinsenosides are major pharmacologically active compounds present in ginseng (Panax ginseng). Among the ginsenosides, 20‐O‐β‐D‐glucopyranosyl‐20(S)‐protopanaxadiol (GPPD) and ginsenoside Rb1 (Rb1) have previously been reported to exhibit anti‐wrinkle effects. In this study, 20(S)‐protopanaxadiol (20(S)‐PPD), an aglycone derivative of the Rb1 metabolite was investigated for its anti‐wrinkle benefit and compared to GPPD and Rb1. The anti‐wrinkle effect of 20(S)‐PPD during solar UV light was investigated using a human skin equivalent model and human keratinocytes. 20(S)‐PPD attenuated solar UV‐induced matrix metalloproteinase (MMP)‐1 expression to a greater extent than GPPD and Rb1. 20(S)‐PPD treatment modulated MMP‐1 mRNA expression and the transcriptional activity of activator protein (AP)‐1, a major transcription factor of MMP‐1. Two upstream signaling pathways for AP‐1, the MEK1/2‐ERK1/2‐p90RSK and MEK3/6‐p38 pathways, were also suppressed. Taken together, these findings highlight the potential of 20(S)‐PPD for further development as a preventative agent for sunlight‐induced skin wrinkle. J. Cell. Biochem. 118: 3756–3764, 2017. © 2017 Wiley Periodicals, Inc.
Retracted: MicroRNA‐126 Inhibits Proliferation, Migration, Invasion, and EMT in Osteosarcoma by Targeting ZEB1
Authors: Jiang Rui , Zhang Chao , Liu Guangyao , Gu Rui , Wu Han 
Published: April 2017

ABSTRACTOsteosarcoma (OS) is a tumor with rapid progression, high metastatic potential and poor clinical prognosis. This study was aimed to investigate the function of miR‐126 in OS cells. The miR‐126 expression in OS cell lines and OS tissues were explored by qRT‐PCR. Then, the effects of miR‐126 on proliferation, cycle, migration, invasion, and transforming growth factor (TGF)‐β1 induced epithelial to mesenchymal transition (EMT) were assessed. Predicted by TargetScan, one of target genes for miR‐126 was verified by luciferase activity assay. Meantime, the mRNA and protein expressions of ZEB1 were assessed by qRT‐PCR and Western blot assay. Subsequently, the effect of ZEB1 silence on miR‐126 down‐regulated cells was also evaluated. Finally, the expressions of key kinases involved in c‐Jun N‐terminal kinase (JNK) and Janus‐activated kinase (JAK)‐1/signal transducer and activator of transcription (STAT)‐3 pathways were detected by Western blot analysis. Result showed that miR‐126 was down‐regulated in OS tissues and cell lines. Overexpression of miR‐126 significantly inhibited cell proliferation, migration, invasion, and TGF‐β1 induced EMT. The effect of miR‐126 knockdown was just the opposite. ZEB1 was predicted and verified as a target gene of miR‐126. Meantime, the influence of miR‐126 knockdown was abrogated by ZEB1 silence. Additionally, the phosphorylation levels of c‐Jun, JNK, JAK1, and STAT3 were down‐regulated in miR‐126 over‐expressed cells, and the effect of miR‐126 knockdown was reversed by ZEB1 silence. In conclusion, miR‐126 inhibits proliferation, migration, invasion and EMT in OS by targeting ZEB1 through inactivation of JNK and JAK1/STAT3 pathways. J. Cell. Biochem. 118: 3765–3774, 2017. © 2017 Wiley Periodicals, Inc.
Short‐Term Hypoxia Reverses Ox‐LDL‐Induced CD36 and GLUT4 Switching Metabolic Pathways in H9c2 Cardiomyoblast Cells
Authors: Chen Yeh‐Peng , Hsu Hsi‐Hsien , Baskaran Rathinasamy , Wen Su‐Ying , Shen Chia‐Yao , Day Cecilia‐Hsuan , Ho Tsung‐Jung , Vijaya Padma Viswanadha , Kuo Wei‐Wen , Huang Chih‐Yang 
Published: April 2017

ABSTRACTHigh levels of circulating low‐density lipoproteins (LDL, plasma proteins that carry cholesterol and triglycerides) are associated with type 2 diabetes, arteriosclerosis, obesity, and hyperlipidemia. In the heart, the accumulation of oxidized low‐density lipoprotein (Ox‐LDL) has been proposed to play a role in the development of cardiovascular disease. We obtain cholesterol from animals and animal‐derived foods such as milk, eggs, and cheese. In previous studies, the ratio of low‐density lipoprotein (LDL) and high‐density lipoprotein (HDL) was shown to be important for our health. High levels of LDL cholesterol lead to atherosclerosis, increasing the risk of heart attack and ischemic stroke. In this study, we utilized Ox‐LDL‐treated H9c2 cardiomyoblast cells as a simulated hyperlipidemia model. CD36 metabolism pathway proteins (phospho‐Akt, SIRT1, PGC1α, PPARα, CPT1β, and CD36) increased at low doses of Ox‐LDL. However, high doses (150 and 200 mg/dL) of Ox‐LDL reduced the levels of these proteins. Interestingly, expression of GLUT4 metabolism pathway proteins (phospho‐PKCζ) were reduced at low doses, while the expression of phospho‐AMPK, phospho‐PI3K, phospho‐PKCζ, GLUT4, and PDH proteins increased at high doses. Ox‐LDL acute treatment induces apoptosis in cardiomyocytes as evidenced by apoptotic nuclei apparition, caspase‐3 activation, and cytochrome c release from mitochondria. In our results, Ox‐LDL induced lipotoxicity in cardiomyocytes, and subsequent exposure to short‐term hypoxia or reversed the Ox‐LDL‐induced metabolic imbalance. The same result was obtained with the pharmacological activation of SIRT1 by resveratrol and si‐PKCζ. The mechanism of metabolic switching during Ox‐LDL lipotoxicity seems to be mediated by SIRT1 and PKC ζ. J. Cell. Biochem. 118: 3785–3795, 2017. © 2017 Wiley Periodicals, Inc.
In Vitro Adult Astrocytes are Derived From Mature Cells and Reproduce in Vivo Redox Profile
Authors: Souza Débora Guerini , Bellaver Bruna , Terra Silvia Resende , Guma Fatima Costa Rodrigues , Souza Diogo Onofre , Quincozes‐Santos André 
Published: April 2017

ABSTRACTAstrocytes are versatile cells involved in synaptic information processing, energy metabolism, redox homeostasis, inflammatory response, and structural support of the brain. Recently, we established a routine protocol of cultured astrocytes derived from adult and aged Wistar rats, which present several different responses compared to newborn astrocytes, commonly used to characterize the role of the astrocytes in the central nervous system. Previous studies hypothesized that astrocyte cultures prepared from adult animals derive from immature precursors present in the adult tissue throughout life. Since our group has already demonstrated that the glial functionality of adult astrocytes differs from newborn cultures, the aim of this study was to confirm that our in vitro astrocytes were derived from mature cells. Therefore, we evaluated cytoskeleton proteins, such as glial fibrillary acidic protein and vimentin, as well as Sox10, an essential marker of immature glial cells, in ex vivo tissue and in in vitro astrocytes from the same animals (1, 90, and 180 days old). In addition, we examined the mitochondrial functionality and the cellular redox homeostasis. Our results suggest that adult and aged astrocytes are derived from mature cells and that changes in mitochondrial parameters in ex vivo tissue were reproduced in in vitro astrocytes. J. Cell. Biochem. 118: 3111–3118, 2017. © 2017 Wiley Periodicals, Inc.
A duplex polymerase chain reaction‐restriction fragment length polymorphism for rapid screening of methylenetetrahydrofolate reductase gene variants: Genotyping in acute leukemia
Authors: Frikha Rim , Bouayed Nouha , Ben Rhouma Bochra , Keskes Leila , Rebai Tarek 
Published: April 2017

BackgroundMethylenetetrahydrofolate reductase (MTHFR; NM_005957.4) is the key enzyme for folate metabolism which plays in DNA biosynthesis and the epigenetic process of DNA methylation. MTHFR gene polymorphisms, the c. 677C>T and c. 1298A>C have been implicated as risk factors for several types of cancers as the acute leukemia.AimWe have optimized a duplex polymerase chain reaction‐restriction fragment length polymorphism assay (PCR‐RFLP) for the simultaneous detection of both variants in acute leukemia patients, from Tunisia.MethodsGenomic DNA was extracted from EDTA‐anticoagulant blood samples from a total of 50 patients suffering from acute leukemia (AL). After DNA extraction, the polymerase chain reaction using specific primers, designed using Primer 3 Software. Restriction Fragment Length Polymorphism (RFLP) was performed in two separate tubes followed by agarose gel electrophoresis.ConclusionThis new method has proved to be a rapid, simple, and reliable method that should facilitate high throughput genotyping of MTHFR polymorphisms in acute leukemia.
Genotyping of commensal Neisseria spp strains by pulsed‐field gel electrophoresis and 16S rRNA gene sequencing
Authors: Mechergui Arij , Achour Wafa , Ben Hassen Assia 
Published: April 2017

BackgroundWe investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis.MethodsIdentification to the genus was done using conventional methods and API NH (bio‐Mérieux®). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI.ResultsFourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty‐three different PFGE patterns were found among the tested strains.ConclusionWe demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.
The Existential Concerns Questionnaire (ECQ)–Development and Initial Validation of a New Existential Anxiety Scale in a Nonclinical and Clinical Sample
Authors: van Bruggen Vincent , ten Klooster Peter , Westerhof Gerben , Vos Joël , de Kleine Elian , Bohlmeijer Ernst , Glas Gerrit 
Published: April 2017

ObjectiveExistential anxiety (EA) is a construct that refers to fears that are provoked by core threats of human existence, such as death, meaninglessness, and fundamental loneliness. The objective of this study was to develop an EA measure that can be used in research and clinical practice.MethodThe Existential Concerns Questionnaire (ECQ) was completed by a nonclinical sample of 389 adults, together with questionnaires measuring death anxiety, intolerance of uncertainty, neuroticism, distress, meaning, and life events. Adaptations were made based on item analysis and factor analysis. A total of 99 adults who had an anxiety and/or depressive disorder completed the final version.ResultsThe ECQ was demonstrated to be essentially unidimensional and showed good reliability and stability. Correlations with other measures were within the expected range of strength, except for a weak association with life events.ConclusionInitial results regarding the psychometric properties of the ECQ are promising.
Development and Initial Evaluation of the Psychometric Properties of the Dialectical Behavior Therapy Barriers to Implementation Scale (BTI‐S)
Authors: Chugani Carla D. , Mitchell Michael E. , Botanov Yevgeny , Linehan Marsha M. 
Published: April 2017

ObjectiveWe examined the psychometric properties of the Dialectical Behavior Therapy (DBT) Barriers to Implementation Scale (BTI‐S).MethodParticipants were clinicians completing a bipartite training, separated by 6 months of self‐study, to implement DBT. Exploratory factor analysis was conducted with data collected from the initial training period (N = 790), while confirmatory factor analysis was based on data from the final training period (N = 660).ResultsThe final model included 26 items and 4 factors with overall acceptable fit, χ2(293, N = 660) = 460.989, p < .001, comparative fit index = .909, Tucker‐Lewis index = .899, root mean square root mean square error of approximation (90% confidence interval) = .015. The internal reliability of the scores was low (range = .56 to .72).ConclusionThe final model fits reasonably well and measures four domains considered important for implementation of DBT. However, the low reliability of the scores indicates that the BTI‐S would be improved with further development.
The Mediating Role of Integration of Loss in the Relationship Between Dissociation and Prolonged Grief Disorder
Authors: Hasson‐Ohayon Ilanit , Peri Tuvia , Rotschild Itai , Tuval‐Mashiach Rivka 
Published: April 2017

ObjectiveFindings from the field of trauma have shown that there is a negative relationship between dissociation and integration of loss into the self‐narrative. At the same time, an increasing amount of literature on grief has stressed the importance of an integrated self‐narrative in the grieving process. Accordingly, the current study examined the possible mediating role played by the extent to which individuals have integrated their loss into their self‐narrative in the relationship between dissociation and symptoms of prolonged grief disorder (PGD).MethodThe Prolonged Grief Disorder Scale (PGD‐13), the Outcome Questionnaire, the Integration of Stressful Life Experiences Scale, the Dissociative Experiences Scale, and a sociodemographic questionnaire were administered to 66 individuals who had lost a loved one.ResultsThe hypothesized mediation model of integration was confirmed.ConclusionThe effect of people's dissociative tendencies on their PGD symptoms seems to occur via the impairment of their ability to integrate the memory of their loss into their general autobiographical memory. Empirical and clinical implications are discussed.
Isolated right ventricular apical hypoplasia characterized by computed tomography and echocardiography
Authors: Zhou Dan , Liao Hai‐Qin , Ouyang Min‐Zhi , Shang Quan‐Liang , Zhang Ming 
Published: April 2017

ABSTRACTIsolated right ventricular apical hypoplasia is an unusual congenital heart disease that has been mentioned in only one report to our knowledge. We describe the case of a 62‐year‐old male patient suffering from recurrent abdominal distention, nausea, and lower extremity edema. The right ventricular morphologic abnormalities as shown by echocardiography and CT were comparable to those of left ventricular apical hypoplasia, suggesting right ventricular apical hypoplasia. However, this speculative diagnosis remains to be confirmed by additional cases. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 46:82–84, 2018;
Prenatal sonographic findings in a case of Wolman's disease
Authors: Blitz Matthew J. , Rochelson Burton , Sood Monica , Bialer Martin G. , Vohra Nidhi 
Published: April 2017

ABSTRACTNo published case of Wolman's disease has described the prenatal sonographic findings. We present a case in which a third‐trimester sonographic examination demonstrated fetal hepatomegaly and bilateral adrenal echogenicity suggestive of diffuse calcification. Wolman's disease, also known as lysosomal acid lipase (LIPA) deficiency, is a rare autosomal‐recessive disorder characterized by complete absence of the LIPA enzyme. The diagnosis of Wolman's disease was made postnatally by biochemical testing, which indicated absence of LIPA enzyme activity and gene sequencing, which confirmed homozygosity for the G66V mutation within the LIPA gene. © 2017 Wiley Periodicals, Inc. J Clin Ultrasound 46:66–68, 2018;
A Decade of Poverty Reduction in Kazakhstan 2001–2009: Growth and/or Redistribution?
Authors: Kudebayeva Alma , Barrientos Armando 
Published: April 2017

AbstractThis paper examines the factors explaining the sustained fall in poverty experienced in Kazakhstan in the period 2001–2009. It examines the contribution of economic growth and redistribution policies to poverty reduction through an analysis of household survey data. It finds that growth has been strongly pro‐poor. Growth was the main driver behind the fall in poverty in the first half of the decade, but redistribution gained in importance in the latter part of the decade. Copyright © 2017 John Wiley & Sons, Ltd.
POP1 might be recruiting its type‐Ia interface for NLRP3‐mediated PYD‐PYD interaction: Insights from MD simulation
Authors: Maharana Jitendra , Vats Ashutosh , Gautam Santwana , Nayak Bibhu Prasad , Kumar Sushil , Sendha Jasobanta , De Sachinandan 
Published: April 2017

AbstractInflammasomes are multiprotein caspase‐activating complexes that enhance the maturation and release of proinflammatory cytokines (IL‐1β and IL‐18) in response to the invading pathogen and/or host‐derived cellular stress. These are assembled by the sensory proteins (viz NLRC4, NLRP1, NLRP3, and AIM‐2), adaptor protein (ASC), and effector molecule procaspase‐1. In NLRP3‐mediated inflammasome activation, ASC acts as a mediator between NLRP3 and procaspase‐1 for the transmission of signals. A series of homotypic protein‐protein interactions (NLRP3PYD:ASCPYD and ASCCARD:CASP1CARD) propagates the downstream signaling for the production of proinflammatory cytokines. Pyrin‐only protein 1 (POP1) is known to act as the regulator of inflammasome. It modulates the ASC‐mediated inflammasome assembly by interacting with pyrin domain (PYD) of ASC. However, despite similar electrostatic surface potential, the interaction of POP1 with NLRP3PYD is obscured till date. Herein, to explore the possible PYD‐PYD interactions between NLRP3PYD and POP1, a combined approach of protein‐protein docking and molecular dynamics simulation was adapted. The current study revealed that POP1's type‐Ia interface and type‐Ib interface of NLRP3PYD might be crucial for 1:1 PYD‐PYD interaction. In addition to type‐I mode of interaction, we also observed type‐II and type‐III interaction modes in two different dynamically stable heterotrimeric complexes (POP1‐NLRP3‐NLRP3 and POP1‐NLRP3‐POP1). The inter‐residual/atomic distance calculation exposed several critical residues that possibly govern the said interaction, which need further investigation. Overall, the findings of this study will shed new light on hitherto concealed molecular mechanisms underlying NLRP3‐mediated inflammasome, which will have strong future therapeutic implications.
Brain iron accumulation in Wilson's disease: A longitudinal imaging case study during anticopper treatment using 7.0T MRI and transcranial sonography
Authors: Dusek Petr , Skoloudik David , Maskova Jana , Huelnhagen Till , Bruha Radan , Zahorakova Daniela , Niendorf Thoralf , Ruzicka Evzen , Schneider Susanne A. , Wuerfel Jens 
Published: April 2017

Level of Evidence: 5Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2018;47:282–285.
Quantifying tumor vascular heterogeneity with DCE‐MRI in complex adnexal masses: A preliminary study
Authors: Thomassin‐Naggara Isabelle , Soualhi Narimane , Balvay Daniel , Darai Emile , Cuenod Charles‐André 
Published: April 2017

PurposeTo evaluate the value of quantifying dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) heterogeneity to characterize adnexal masses.Materials and MethodsOur database was retrospectively queried to identify all surgically proven adnexal masses characterized with a 1.5T DCE‐MRI between January 1st 2008 and February 28th 2010 (n = 113 masses, including 52 benign, 11 borderline, and 50 invasive malignant tumors). The solid component of the adnexal mass was segmented. Quantitative analysis with a compartmental model was performed to calculate microvascular parameters, including tissue blood flow (FT), blood volume fraction (Vb), lag time (DAT), interstitial volume fraction (Ve), permeability–surface area product (PS), and relative area under curve (rAUC), were calculated. Then heterogeneity parameters were evaluated using the analysis of the evolution of the standard deviation (SD) of signal intensities on DCE‐MRI series. The area under the receiver operating characteristic (AUROC) curve was calculated to assess the overall discrimination of parameters.ResultsMalignant tumors displayed higher FT, Vb, and rAUC and lower DAT than benign tumors (P = 0.01, P < 0.0001, and P < 0.0001, respectively). Invasive malignant tumors displayed lower Vb and rAUC than borderline tumors (P < 0.01). After injection, whenever the heterogeneity parameter was considered, malignant tumors were more heterogeneous than benign tumors, invasive tumors were more heterogeneous than borderline ovarian tumors, and malignant tumors with carcinomatosis were more heterogeneous than tumors without carcinomatosis (P < 0.001). The most discriminant parameter was the SD during the 90 seconds after injection related to arterial input function (ΔSDEARLY/AIF) with an AUROC between 0.715 and 0.808.ConclusionThis study proposes heterogeneity parameters as a new tool with a potential for clinical application, given that the technique uses routine imaging sequences.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2017;46:1776–1785.
Lung perfusion: MRI vs. SPECT for screening in suspected chronic thromboembolic pulmonary hypertension
Authors: Johns Christopher S. , Swift Andrew J. , Rajaram Smitha , Hughes Paul J.C. , Capener David J. , Kiely David G. , Wild James M. 
Published: April 2017

PurposeTo assess the diagnostic accuracy of magnetic resonance imaging (MRI) perfusion against perfusion single photon emission tomography (SPECT) screening for chronic thromboembolic pulmonary hypertension (CTEPH). Ventilation/perfusion (V/Q) scintigraphy is recommended to screen for suspected CTEPH. It has previously been shown that 3D dynamic contrast‐enhanced (DCE) lung perfusion MRI has a similar sensitivity for diagnosing CTEPH in comparison to planar perfusion scintigraphy; however, planar scintigraphy has now been largely replaced by SPECT, due to higher spatial resolution and sensitivity.Materials and MethodsConsecutive patients with suspected CTEPH or unexplained pulmonary hypertension attending a referral center, who underwent lung DCE perfusion MRI at 1.5T, perfusion SPECT, and computed tomography pulmonary angiography (CTPA) within 14 days of right heart catheterization, from April 2013 to April 2014, were included. DCE‐MR, SPECT, and CTPA were independently analyzed by two blinded radiologists. Disagreements were corrected by consensus. The gold standard reference for the diagnosis of chronic thromboemboli was based on a review of multimodality imaging and clinical findings.ResultsIn all, 74 patients with suspected CTEPH underwent all three modalities. Forty‐six were diagnosed with CTEPH (36) or chronic thromboembolic disease (CTED) (10). 3D DCE perfusion MRI correctly identified all patients (sensitivity of 100%), compared with a 97% sensitivity for SPECT.ConclusionDCE lung perfusion MRI has increased sensitivity when compared with perfusion scintigraphy in screening for CTEPH. As MRI does not use ionizing radiation, it should be considered as a first‐line imaging modality in suspected CTEPH.Level of Evidence: 3Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2017;46:1693–1697.