bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activating STAT3 mutations in CD8+ T-cells correlate to serological positivity in rheumatoid arthritis
Authors: Moosic Katharine B. , Olson Thomas L. , Freijat Mark , Khalique Samara , Hamele Cait E. , Shemo Bryna , Boodoo Jesse , Baker William , Khurana Gitanjali , Schmachtenberg Matthew , Duffy Tristin , Ratan Aakrosh , Darrah Erika , Andrade Felipe , Jones Marieke , Olson Kristine C. , Feith David J. , Kimpel Donald L. , Loughran Thomas P. 
Published: October 2024

ObjectivesLarge granular lymphocyte (LGL) leukemia is a rare hematologic malignancy characterized by clonal expansion of cytotoxic T-cells frequent somatic activating STAT3 mutations. Based on the disease overlap between LGL leukemia rheumatoid arthritis (RA)a putative role for CD8+ T-cells in RA we hypothesized that STAT3 mutations may be detected in RA patient CD8+ T-cells correlate with clinical characteristics.MethodsBlood samples, clinical parameters, and demographics were collected from 98 RA patients and 9 healthy controls (HCs). CD8+ cell DNA was isolated and analyzed via droplet digital (dd)PCR to detect STAT3 mutations common in LGL leukemia: Y640F, D661Y, and the S614 to G618 region. STAT3 data from 99 HCs from a public dataset supplemented our 9 HCs.ResultsRA patients had significantly increased presence of STAT3 mutations compared to controls (Y640F p=0.0005, D661Y p=0.0005). The majority of these were low variant allele frequency (VAF) (0.008-0.05%) mutations detected in a higher proportion of the RA population (31/98 Y640F, 17/98 D661Y) vs. HCs (0/108 Y640F, 0/108 D661Y). In addition, 3/98 RA patients had a STAT3 mutation at a VAF &gt;5% compared to 0/108 controls. Serological markers, RF and anti-CCP positivity, were more frequently positive in RA patients with STAT3 mutation relative to those without (88% vs 59% RF, p=0.047; 92% vs 58% anti-CCP, p=0.031, respectively).ConclusionsSTAT3 activating mutations were detected in RA patient CD8+ cells and associated with seropositivity. Thus, STAT3 activating mutations may play a role in disease pathogenesis in a subset of RA patients.
Semi-quantitative CT score reflecting the degree of pulmonary infection as a risk factor of hypokalemia in COVID-19 patients: a cross-sectional study
Authors: Li Ru , Wu Baofeng , Yang Xifeng , Liu Botao , Zhang Jian , Li Mengnan , Zhang Yi , Qiao Ying , Liu Yunfeng 
Published: October 2024

BackgroundHypokalemia is a common electrolyte disorder observed in patients afflicted with coronavirus disease 2019 (COVID-19). When COVID-19 is accompanied by pulmonary infection, chest computed tomography (CT) is the preferred diagnostic modality. This study aimed to explore the relationship between CT semi-quantitative score reflecting the degree of pulmonary infection and hypokalemia from COVID-19 patients.MethodsA single-center, cross-sectional study was conducted to investigate patients diagnosed with COVID-19 between December 2022 and January 2023 who underwent chest CT scans upon admission revealing typical signs. These patients were categorized into two groups based on their blood potassium levels: the normokalemia group and the hypokalemia group. Medical history, symptoms, vital signs, laboratory data, and CT severity score were compared. Binary regression analysis was employed to identify risk factors associated with hypokalemia in COVID-19 patients with pulmonary infection.ResultsA total of 288 COVID-19 patients with pulmonary infection were enrolled in the study, of which 68 (23.6%) patients had hypokalemia. The CT severity score was found to be higher in the hypokalemia group compared to the normokalemia group [4.0 (3.0–5.0) vs. 3.0 (2.0–4.0), p = 0.001]. The result of binary logistic regression analysis revealed that after adjusting for sex, vomiting, sodium, and using potassium-excretion diuretics, higher CT severity score was identified as an independent risk factor for hypokalemia (OR = 1.229, 95% CI = 1.077–1.403, p = 0.002).ConclusionIn this cohort of patients, semi-quantitative CT score reflecting the degree of pulmonary infection may serve as a risk factor of hypokalemia in COVID-19 patients.
Design, synthesis, and biological evaluation of 2,4-dimorpholinopyrimidine-5-carbonitrile derivatives as orally bioavailable PI3K inhibitors
Authors: Huang Daowei , Yang Jixia , Zhang Qingwei , Zhou Xiaolei , Wang Yanbo , Shang Zhenhua , Li Jianqi , Zhang Baoyin 
Published: October 2024

IntroductionPhosphoinositide-3-kinase (PI3K) is overexpressed in many tumors and is, thus, an ideal target for cancer treatments. Accordingly, there is an urgent need for the development of PI3K inhibitors with high potency and low toxicity.MethodsIn this study, we designed and synthesized a series of 2,4-dimorpholinopyrimidine-5-carbonitrile derivatives, which were evaluated for their PI3K inhibitory potency.Results and discussionCompound 17p demonstrated comparable PI3Kα inhibitory activity (IC50: 31.8 ± 4.1 nM) to the positive control, BKM-120 (IC50: 44.6 ± 3.6 nM). In addition, 17p showed significant inhibitory activity against PI3Kδ (IC50: 15.4 ± 1.9 nM) and significant isoform selectivity against PI3Kβ, PI3Kγ, and mTOR. Furthermore, 17p exhibited good antiproliferative activities against cancer cell activity and good safety in the Ames and hERG tests while having outstanding liver microsomal stability in vitro, with half-lives of 38.5 min in rats and 127.9 min in humans. In addition, in an apoptosis assay, 17p could induce dose-dependent cytotoxicity in the ovarian cancer cell line A2780. In a pharmacokinetic study, 17p was stable (T½: 2.03 h) and showed high bioavailability (46.2%). Collectively, these results indicate that 17p could be a promising PI3K agent for cancer treatment.
Comparative Evaluation of the Chemical Components and Anti-Inflammatory Potential of Yellow- and Blue-Flowered Meconopsis Species: M. integrifolia and M. betonicifolia
Authors: Cheng Peizhao , Gan Ruixi , Wang Cong , Xu Qian , Norbu Kelsang , Zhou Feng , Kong Sixin , Jia Zhuoma , Jiabu Dawa , Feng Xin , Wang Junsong 
Published: October 2024

Background/Objectives: Meconopsis has long been used in traditional Tibetan medicine to treat various inflammatory and pain-related conditions. However, blue-flowered Meconopsis (M. betonicifolia) is becoming increasingly scarce due to overharvesting. As a potential alternative, yellow-flowered Meconopsis (M. integrifolia) shows promise but requires comprehensive characterization. This study aimed to evaluate and compare the anti-inflammatory potential of yellow- and blue-flowered Meconopsis species. Methods: Liquid chromatography–mass spectrometry (LC-MS) techniques were used to analyze the chemical profiles of yellow- and blue-flowered Meconopsis. Putative targets of shared constituents were subjected to GO and disease enrichment analysis. The LPS-induced RAW264.7 macrophage model was employed to assess anti-inflammatory effects. Metabolomics was applied to gain mechanistic insights. Results: LC-MS revealed over 70% chemical similarity between species. Enrichment analysis associated targets with inflammation-related pathways. In macrophage assays, both species demonstrated dose-dependent antioxidative and anti-inflammatory activities, with yellow Meconopsis exhibiting superior efficacy. Metabolomics showed modulation of key inflammatory metabolic pathways. Conclusions: This integrative study validated yellow-flowered Meconopsis as a credible alternative to its blue-flowered counterpart for anti-inflammatory applications. Metabolic profiling provided initial clues regarding their multi-targeted modes of action, highlighting their potential for sustainable utilization and biodiversity conservation.
Implementación del sotfware MICMACC y MACTOR para identificar los factores y actores clave que más impactan el uso de las TIC en la enseñanza y el aprendizaje
Authors: Pertuz Leonardo Antonio Diaz , Fuentes Greidys María Cardozo , Fuentes Margareth Gicela Cardozo , Canoles Fernando Fabio Fernández , Argumedo Daniela Sierra , Román Mateo Aviles 
Published: October 2024

El objetivo de la presente investigación es reconocer la importancia del uso pedagógico de las TIC, en los procesos de enseñanza - aprendizaje, analizando desde el método Micmac y Mactor, para lo cual se hace el diligenciamiento de las matrices Árbol de competencias de Marc Giget / Cambios esperados para el futuro / DOFA / Estereotipos, luego se definen y describen los factores, se registran en el software MICMAC, posteriormente se determinan los actores y se evalúa su incidencia con los objetivos planteados en el software MACTOR, y por último se realiza un rastreo bibliográfico en bases de datos académica como: Scielo, Redalyc, Google académico, Dialnet entre otros, los cuales se utilizan para el análisis. Los resultados indican que las variables de mayores influencias son: Era digital, brechas digitales, niveles de competencias tecnológicas, alfabetización digital, idoneidad docente, mediación del aprendizaje, políticas educativas nacionales, implementación de las TIC, conectividad y los nativos digitales, por lo que se puede concluir que las mediaciones TIC son herramientas que inciden positivamente en los procesos de enseñanza-aprendizaje, resaltando los cambios en estudiantes y docentes al momento de contextualizar los nuevos aprendizajes.
Persistência do quarto arco aórtico direito em um canino
Authors: Mendonça Hugo Leite Serrano de , Aleixo Grazielle Anahy de Souza , Martir Ediclesio Andrade , Guimarães Evelen da Silva , Lima Déborah Cavalcante de Aliança , Lima Maynara Kayla Ferreira , Siqueira Filho Robério Silveira de , Souza Wandsson João da Silva e 
Published: October 2024

A persistência do quarto arco aórtico direito (PAAD) é uma afecção que se inicia na fase embrionária através do mal desenvolvimento das estruturas vasculares dos arcos aórticos. Dentre todas as anomalias dos anéis vasculares, a PAAD é a mais comum, correspondendo a 95% de toda casuística. Costuma ter diagnóstico precoce, em especial no período de desmame e introdução da dieta seca, visto que tal má formação leva a uma constrição esofágica dificultando a passagem do alimento. O diagnóstico é baseado na história clínica, sinais clínicos e exames de imagem, sendo a intervenção cirúrgica o tratamento de eleição. O objetivo do presente trabalho é relatar um caso de PAAD em um cão de 3 meses de idade, macho, beagle, atendido no Hospital Veterinário Universitário da UFRPE, com histórico de regurgitação e emagrecimento. Após exame físico, foram solicitados exames de imagem e o paciente foi diagnosticado com possível persistência do quarto arco aórtico direito. Posteriormente, foram solicitados os exames pré operatórios e realizado o encaminhamento para a cirurgia. O paciente foi submetido a uma toracotomia pelo quarto espaço intercostal esquerdo para identificação, ligadura e transecção do ducto arterioso e dilatação esofágica através de uma sonda de Foley. Após o procedimento, o paciente foi encaminhado para uma unidade de cuidados intensivos veterinários, recebendo alta após 4 dias. O primeiro retorno foi realizado com 15 dias e o paciente apresentava evolução e cicatrização da ferida cirúrgica, recebendo alta médica. Concluindo-se que a cirurgia foi eficaz no tratamento da afecção.
A dinâmica fluvial e o regime hidrológico e de sedimentos em bacia rural
Authors: Conceição Gerson , Miranda Jr Guilherme Xavier de 
Published: October 2024

Este estudo aprofundou o entendimento do regime hídrico e a perda de solo através dos sedimentos em suspensão em bacia rural com intensa produção agropecuária. Identificou-se que há ocorrências de chuvas significativas anuais de até 2777 mm na bacia hidrológica, sendo que nestes períodos chuvosos (ano de El niño) há intensidade de chuvas mensais significativas que podem alcançar 300% acima da média mensal, provocando fluxos superficiais difusos significantes carreando para o rio elevados volumes de sedimentos, ultrapassando em 50% a média mensal de descarga sólida que é transportada para fora da bacia, apresentando risco de degradação do solo e das águas superficiais que causam alterações na qualidade de água. A Bacia Hidrográfica do Lajeado do Fragosos apresentou vazão média anual na foz de cerca de 1,2 m3.s-1, onde se observa uma forte correlação entre as variáveis de precipitação e descarga líquida, com baixo tempo de concentração, na ordem de 3,8 horas. As informações sobre as características do curso d´água, por meio da curva de permanência, permitiu caracterizar que o comportamento do curso d´água da bacia é um regime pluvial fortemente torrencial perene. A análise da curva de permanência da vazão mostrou que pode ser uma boa ferramenta para avaliar o armazenamento da água do solo e o regime pluvial de um curso d´água.
Update of the non-marine (terrestrial & freshwater) molluscs systematic inventory occurring in Santa Catarina State/ SC, Central Southern Brazil region
Authors: Agudo-Padrón Aisur Ignacio 
Published: October 2024

Introduction: The survey of the mollusc fauna (malacofauna) of a geopolitical region (State) is important from several aspects, reaching several important sectors of knowledge. So if all regions could do the same we would have within, a few years, expanded and completed the catalog of molluscs in Brazil, with much still to be added with regard to terrestrial and freshwater fauna. Objective: Updated general rеᴠiеw of the inventory of non-marine molluscs occurring in the Santa Catarina State/ SC, subtropical Central Southern portion of the Brazilian territory. Methodology: Organization of a occurring State species taxonomic checklist and their corresponding reference database, comprising the regional knowledge formally produced around the theme at least since the year 2003. Results and Conclusions: Confirmed and recorded regional total of 241 species (202 Gastropoda & 39 Bivalvia), among which at least 86 (79 Gastropoda & 7 Bivalvia) exhibit radicals synonymical (among others) changes recently reported through the global platform "MolluscaBase" and available literature of recent production, taxonomically included all in 95 genera (83 Gastropoda & 12 Bivalvia) and 40 families (35 Gastropoda & 5 Bivalvia), with aggregated additional information about their known biogeographical distribution in the territorial space of the State.
Molecular dynamics study of the effect of composition on elastic properties of silicon oxynitride films
Authors: Miyazaki Sakurako , Sakakima Hiroki , Ogawa Keigo , Izumi Satoshi 
Published: October 2024

Abstract
Understanding the mechanical properties of silicon oxynitride (a-SiON), a key insulating material, is vital for electronic device design and reliability. Though the effects of fabrication conditions on a-SiON have been studied, the underlying relationship between its atomic-scale structure and mechanical properties remains unclear. This study investigates the relationship between elasticity and atomic-scale structures in a-SiON by molecular dynamics simulations with a universal graph neural network interatomic potential. The bulk modulus increases from 49 to 150 GPa with higher N content. N atoms form N2 molecules under O-rich conditions, hindering bulk modulus increase, and form an Si3N4-like network under O-poor conditions, enhancing bulk modulus. Formation energy calculations indicate N2 formation is preferable under O-rich conditions. Meanwhile, under O-poor conditions, Si–N bond formation is preferable, which reinforces a-SiON by increasing bond density. The findings suggest realizing O-poor conditions is crucial for highly elastic insulating films.
Double localized surface plasmon‒enhanced-second-harmonic generation behavior of equilateral triangular aluminum nanoprisms
Authors: Sugita Atsushi , Muroi Kennshin , Tamotsu Sohta 
Published: October 2024

Abstract
This paper presents second-harmonic generation (SHG) behaviors for different-sized equilateral triangular aluminum nanoprisms. These behaviors were observed under the double localized surface plasmon (LSP) resonance condition. The SHG conversion efficiency was substantially enhanced when the excitation and SHG wavelengths were simultaneously tuned to the LSP-resonance wavelengths. The fundamental and higher-order LSP modes could be used to enhance the SHG conversion efficiency. The nonlinear light‒matter interaction associated with the double LSP resonance was numerically analyzed while focusing on the spatial overlap of the near-field distributions between the excitation and SHG wavelengths. The double LSP resonance enhanced SHG behaviors have been realized by using several elegantly designed plasmonic metal nanostructures consisting of multi-elements. Our present study demonstrates this double LSP resonance enhanced SHG behavior from a single, simple-shape triangular aluminum nanoprism.
El consumo incidental de noticias en los estudiantes universitarios durante la pandemia
Authors: Barreno Cedeño Rafael , Trámpuz Juan Pablo , Vélez Álava Nancy 
Published: October 2024

El consumo incidental de noticias es encontrar por casualidad información de carácter informativo según los hábitos de navegación, siendo esta una práctica cada vez más habitual, principalmente, entre los jóvenes. El presente trabajo de investigación tiene como objetivo analizar el consumo noticioso por internet en los estudiantes de la Universidad Laica Eloy Alfaro de Manabí, durante la pandemia de Covid-19. Para ello se empleó el método bibliográfico y analítico, mediante la aplicación de una encuesta en línea a 378 estudiantes de la Uleam de la ciudad de Manta, durante los meses de agosto y septiembre de 2021. Con los resultados recopilados, se pudo determinar que la mayor parte de los encuestados realizan un consumo incidental de noticias, práctica que realizan con bajos niveles de participación. Adicionalmente, los medios de comunicación se convierten en la principal fuente para contrastar la información cuando los usuarios tienen dudas sobre su veracidad, por lo que se concluye que es fundamental mantener los criterios de calidad en las empresas periodísticas para asumir efectivamente este rol.
PENGARUH SUBSTITUSI TEPUNG TERIGU DENGAN TEPUNG BONGGOL PISANG TERHADAP SERAT PANGAN, AKTIVITAS ANTIOKSIDAN, DAN SIFAT ORGANOLEPTIK COOKIES
Authors: Nuha Jihaan Alyaa Faadhila , Fauziyah A'immatul 
Published: October 2024

Banana corm had high fiber content and antioxidant activity. This search aimed to analyze of effect of cookie substitution with banana corm on its fiber content, antioxidant activity, and organoleptic. This study used a randomized design method (RAL) with one factor (wheat flour substitution with banana corm with proportion banana corm flour: wheat flour are 25 percent: 75 percent (F1), 50 percent: 50 percent (F2), 75 percent: 25 percent (F3), and 100 percent : 0 percent (F4)). The result showed that substitution using banana corm increased fiber content significantly (p=0,038) but did not affect antioxidant activity (p=0,136). The substitution also significantly affected aroma (p=0,028), taste (p=0,000), and texture (p=0,000) but did not affect significantly the color of cookies (P=0,078). The best formula was F4 which contained ash, fat, protein, carbohydrate, dietary fiber, and antioxidant activity were 6.09 percent, 21.43 percent, 6.10 percent, 59.57 percent, 29.31 percent, and 42536.20 ppm respectively.
Keywords: antioxidant activity, banana corm flour, cookies, dietary fiber

ABSTRAK
Tepung bonggol pisang mengandung tinggi serat pangan dan aktivitas antioksidan. Tujuan penelitian ini untuk menganalisis pengaruh substitusi tepung bonggol pisang terhadap kandungan serat pangan, aktivitas antioksidan, dan sifat organoleptik cookies. Metode yang digunakan dalam penelitian ini ialah Rancangan Acak Lengkap (RAL) dengan satu faktor (substitusi tepung terigu dengan tepung bonggol pisang dengan proporsi tepung bonggol pisang : tepung terigu 25 persen : 75 persen (F1), 50 persen : 50 persen (F2), 75 persen : 25 persen (F3), dan 100 persen : 0 persen (F4). Hasil penelitian menunjukkan bahwa cookies dengan substitusi tepung bonggol pisang berpengaruh nyata (p=0,038) terhadap kenaikan kadar serat pangan tetapi tidak terhadap aktivitas antioksidan (p=0,136). Substitusi tepung bonggol pisang juga berpengaruh nyata terhadap tingkat kesukaan panelis terhadap aroma (p=0,028), rasa (p=0,000), dan tekstur cookies (p=0,000) namun tidak berpengaruh nyata terhadap tingkat kesukaan panelis terhadap warna cookies (p=0,078). Formula cookies terbaik adalah F4 yang mengandung kadar air (6,09%), kadar abu (6,82%), lemak (21,43%), Protein (6,10%), karbohidrat (59,57%), kadar serat pangan (29,31%), dan aktivitas antioksidan (42.536,2 ppm).
Kata kunci: aktivitas antioksidan, cookies, tepung bonggol pisang, serat pangan
Analisis Komparatif Perlindungan Investor antara POJK No. 16/2021 and POJK No. 57/2020 pada Securities Crowdfunding (SCF) di Indonesia
Authors: Syamsul Yelia , Sapari Papang 
Published: October 2024

Tulisan ini membahas analisis komparatif perlindungan investor dalam Securities Crowdfunding (SCF) di Indonesia, khususnya antara Peraturan Otoritas Jasa Keuangan POJK No. 16/2021 dan POJK No. 57/2020. Dengan kemajuan teknologi yang mendorong inovasi pendanaan, SCF menawarkan alternatif bagi investor untuk berpartisipasi dalam pendanaan proyek dengan risiko yang bervariasi POJK No. 16/2021 hadir sebagai revisi yang bertujuan untuk memperkuat perlindungan investor melalui mekanisme transparansi dan keamanan yang lebih ketat. Melalui metode penelitian yuridis normatif, artikel ini mengevaluasi efektivitas ketentuan yang diatur dalam kedua regulasi tersebut, serta mencermati tantangan yang dihadapi oleh investor ritel.
Hasil analisis menunjukkan bahwa POJK No. 16/2021 memberikan perlindungan yang lebih komprehensif, termasuk kewajiban penggunaan escrow account, batasan investasi untuk investor ritel, serta kewajiban laporan berkala yang lebih rinci. Meskipun demikian, tantangan tetap ada, terutama terkait pemahaman investor terhadap risiko yang terlibat dan penerapan regulasi di lapangan. Penelitian ini diharapkan dapat memberikan kontribusi dalam pengembangan kebijakan yang lebih baik untuk melindungi investor dalam industri SCF yang terus berkembang.
Implementation of A Fuzzy Logic for Early Detecting of A Pregnant Women Risk of Hypertension
Authors: Fuady Khairul , Handiana Cut Mainy , Zulisa Eva 
Published: October 2024

Hypertension is one of the diseases that can cause fatal effects to health, especially for pregnant women. Hypertension during pregnancy can cause serious effects such as pre-eclampsia and eclampsia which can threaten the lives of pregnant women and fetuses. The purpose of this study is to determine the input and output variables related to hypertension during pregnancy and used for analysis using Tsukamoto FIS to determine risk factors for hypertension in pregnant women. This study uses input variables in the form of maternal age, systolic blood pressure (SBP), diastolic blood pressure (DBP), history of hypertension and genetic. Furthermore, it is analyzed using fuzzy logic through the process of fuzzyfication, inference engine and defuzzyfication. The examination of data samples of pregnant women with systolic and diastolic blood pressure categories included in the high category or potentially have hypertension. The Z value (defuzzyfication) shows that the output is in the category of severe hypertension so that the patient needs immediate treatment by medical personnel. The results showed that the risk of a mother having hypertension can be obtained through Tsukamoto FIS in the form of concrete values that can describe risk factors in the form of normal, hypertension and severe hypertension as well as recommended actions related to these risk factors.