bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone marrow stromal cell therapy for ischemic stroke: A meta-analysis of randomized control animal trials
Authors: Wu Qing , Wang Yuexiang , Demaerschalk Bart M , Ghimire Saruna , Wellik Kay E , Qu Wenchun 
Published: October 2016

Background Results of animal studies assessing efficacy of bone marrow stromal cell therapy for ischemic stroke remain inconsistent. Aims The aims are to assess efficacy of bone marrow stromal cell therapy for ischemic stroke in animal studies. Methods Randomized controlled animal trials assessing efficacy of bone marrow stromal cell therapy were eligible. Stroke therapy academic industry round table was used to assess methodologic quality of included studies. Primary outcomes were total infarction volume and modified Neurological Severity Score. Multiple prespecified sensitivity analyses and subgroup analyses were conducted. Random effects models were used for meta-analysis. Results Thirty-three randomized animal trials were included with a total of 796 animals. The median quality score was 6 (interquartile range, 5–7). Bone marrow stromal cell therapy decreased total infarction volume (standardized mean difference, 0.897; 95% confidence interval, 0.553–1.241; P < .001) at follow-up. Overall standardized mean difference between animals treated with bone marrow stromal cell and controls was 2.47 (95% confidence interval, 1.84–3.11; P < .001) for modified Neurological Severity Score; 1.27 (95% confidence interval, 0.72–1.82; P < .001) for adhesive removal test; and 2.13 (95% confidence interval, 0.65–3.61; P < .001) for rotarod test. Significant heterogeneity among studies was observed. Effect of all outcomes stayed significant in various sensitivity analyses and subgroup analyses, except in a few subgroup analyses with small sample size or with short time follow-up. No significant difference between groups was observed except for study location, in which significantly larger estimates were found in Asian countries. On the basis of this meta-analysis, larger sample sizes are warranted for future animal studies. Conclusions Bone marrow stromal cell therapy significantly decreased total infarction volume and increased neural functional recovery in randomized controlled animal models of ischemic stroke.
Interleukin 32: a novel player in the control of infectious diseases
Authors: Ribeiro-Dias Fátima , Saar Gomes Rodrigo , de Lima Silva Lucas Luiz , dos Santos Jéssica Cristina , Joosten Leo A B 
Published: October 2016

Abstract
Interleukin 32 (IL-32) is a proinflammatory cytokine, expressed as 9 distinct isoforms. The most active isoform is the predominantly intracellular-functioning IL-32γ. Involvement of IL-32 in infectious diseases is increasingly being appreciated. Production of IL-32 promotes pathways that serve to control bacterial infection, especially those caused by mycobacteria. A similar role for this cytokine is observed in the cellular response to viral infections. In addition to its protective effects against microorganisms, IL-32 is involved in immunopathogenesis of some infectious diseases. In parasitic diseases, it has been demonstrated that this cytokine is induced by Leishmania infection. In this review, we summarize the present data on the role of IL-32 in infectious diseases, highlighting this cytokine as new target for control of infections.
Biochemical pathways supporting beta-lactam biosynthesis in the springtail Folsomia candida
Authors: Suring Wouter , Mariën Janine , Broekman Rhody , van Straalen Nico M. , Roelofs Dick 
Published: October 2016

ABSTRACT
Recently, an active set of beta-lactam biosynthesis genes was reported in the genome of the arthropod springtail Folsomia candida (Collembola). Evidence was provided that these genes were acquired through horizontal gene transfer. However, successful integration of fungal- or bacterial-derived beta-lactam biosynthesis into the metabolism of an animal requires the beta-lactam precursor L-α-aminoadipic acid and a phosphopantetheinyl transferase for activation of the first enzyme of the pathway, δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine synthetase (ACVS). In this study, we characterized these supporting pathways and their transcriptional regulation in F. candida. We identified one phosphopantetheinyl transferase and three pathways for L-α-aminoadipic acid production, distinct from the pathways utilized by microorganisms. We found that after heat shock, the phosphopantetheinyl transferase was co-regulated with ACVS, confirming its role in activating ACVS. Two of the three L-α-aminoadipic acid production pathways were downregulated, while PIPOX, an enzyme participating in the pipecolate pathway, was slightly co-regulated with ACVS. This indicates that L-α-aminoadipic acid may not be a limiting factor in beta-lactam biosynthesis in F. candida, in contrast to microorganisms. In conclusion, we show that all components for L-α-aminoadipic acid synthesis are present and transcriptionally active in F. candida. This demonstrates how springtails could have recruited native enzymes to integrate a beta-lactam biosynthesis pathway into their metabolism after horizontal gene transfer.
Early molecular events during retinoic acid induced differentiation of neuromesodermal progenitors
Authors: Cunningham Thomas J. , Colas Alexandre , Duester Gregg 
Published: October 2016

ABSTRACTBipotent neuromesodermal progenitors (NMPs) residing in the caudal epiblast drive coordinated body axis extension by generating both posterior neuroectoderm and presomitic mesoderm. Retinoic acid (RA) is required for body axis extension, however the early molecular response to RA signaling is poorly defined, as is its relationship to NMP biology. As endogenous RA is first seen near the time when NMPs appear, we used WNT/FGF agonists to differentiate embryonic stem cells to NMPs which were then treated with a short 2-h pulse of 25 nM RA or 1 µM RA followed by RNA-seq transcriptome analysis. Differential expression analysis of this dataset indicated that treatment with 25 nM RA, but not 1 µM RA, provided physiologically relevant findings. The 25 nM RA dataset yielded a cohort of previously known caudal RA target genes including Fgf8 (repressed) and Sox2 (activated), plus novel early RA signaling targets with nearby conserved RA response elements. Importantly, validation of top-ranked genes in vivo using RA-deficient Raldh2−/− embryos identified novel examples of RA activation (Nkx1-2, Zfp503, Zfp703, Gbx2, Fgf15, Nt5e) or RA repression (Id1) of genes expressed in the NMP niche or progeny. These findings provide evidence for early instructive and permissive roles of RA in controlling differentiation of NMPs to neural and mesodermal lineages.
Strigolactone regulates shoot development through a core signalling pathway
Authors: Bennett Tom , Liang Yueyang , Seale Madeleine , Ward Sally , Müller Dörte , Leyser Ottoline 
Published: October 2016

ABSTRACTStrigolactones are a recently identified class of hormone that regulate multiple aspects of plant development. The DWARF14 (D14) α/β fold protein has been identified as a strigolactone receptor, which can act through the SCFMAX2 ubiquitin ligase, but the universality of this mechanism is not clear. Multiple proteins have been suggested as targets for strigolactone signalling, including both direct proteolytic targets of SCFMAX2, and downstream targets. However, the relevance and importance of these proteins to strigolactone signalling in many cases has not been fully established. Here we assess the contribution of these targets to strigolactone signalling in adult shoot developmental responses. We find that all examined strigolactone responses are regulated by SCFMAX2 and D14, and not by other D14-like proteins. We further show that all examined strigolactone responses likely depend on degradation of SMXL proteins in the SMXL6 clade, and not on the other proposed proteolytic targets BES1 or DELLAs. Taken together, our results suggest that in the adult shoot, the dominant mode of strigolactone signalling is D14-initiated, MAX2-mediated degradation of SMXL6-related proteins. We confirm that the BRANCHED1 transcription factor and the PIN-FORMED1 auxin efflux carrier are plausible downstream targets of this pathway in the regulation of shoot branching, and show that BRC1 likely acts in parallel to PIN1.
Vps34 regulates Rab7 and late endocytic trafficking through recruitment of the GTPase-activating protein Armus
Authors: Jaber Nadia , Mohd-Naim Noor , Wang Ziqing , DeLeon Jennifer L. , Kim Seong , Zhong Hua , Sheshadri Namratha , Dou Zhixun , Edinger Aimee L. , Du Guangwei , Braga Vania M. M. , Zong Wei-Xing 
Published: October 2016

ABSTRACT
The class III phosphoinositide 3-kinase (PI3K) Vps34 (also known as PIK3C3 in mammals) produces phosphatidylinositol 3-phosphate [PI(3)P] on both early and late endosome membranes to control membrane dynamics. We used Vps34-deficient cells to delineate whether Vps34 has additional roles in endocytic trafficking. In Vps34−/− mouse embryonic fibroblasts (MEFs), transferrin recycling and EEA1 membrane localization were unaffected despite elevated Rab5-GTP levels. Strikingly, a large increase in Rab7-GTP levels, an accumulation of enlarged late endosomes, and decreased EGFR degradation were observed in Vps34-deficient cells. The hyperactivation of Rab7 in Vps34-deficient cells stemmed from the failure to recruit the Rab7 GTPase-activating protein (GAP) Armus (also known as TBC1D2), which binds to PI(3)P, to late endosomes. Protein–lipid overlay and liposome-binding assays reveal that the putative pleckstrin homology (PH) domain in Armus can directly bind to PI(3)P. Elevated Rab7-GTP led to the failure of intraluminal vesicle (ILV) formation and lysosomal maturation. Rab7 silencing and Armus overexpression alleviated the vacuolization seen in Vps34-deficient cells. Taken together, these results demonstrate that Vps34 has a previously unknown role in regulating Rab7 activity and late endosomal trafficking.
The effect of adolescent-parent relationship enhancement program on adolescent perceived parental acceptance / rejection levels and family functions<p>Ergen-ebeveyn ilişki geliştirme programının ergenlerin algıladıkları ebeveyn kabul/red düzeylerine ve aile işlevlerine etkisi
Authors: Epli Koç Hatice , Kaygusuz Canani 
Published: October 2016

The aim of this research was to study the effect of adolescent-parent relationship enhancement program on adolescent perceived parental acceptance / rejection levels and family unfctions. The experimental group and control group of the research consist of 18 pairs of adolescent and parents.In the study, Parental Acceptance-Rejection Questionnaire(PARQ/Child) was used and the Family Assessment Scale(FAD). Both the experimental and control groups were given these scale as pre-test and post-test. The sessions lasted approximately 120 minutes for 10 weeks. Nothing has been applied toy he control groups. For the statistical analyses of the data, the “2x2 two way ANOVA for Repeated Measures” which is suitable for using with mixed designs was employed. In accordance with the findings gathered from the study, perceived parental rejection scores of adolescents in the experimental group were decreased more as compared with the adolescents in the control group. Moreover, perceived unhealthy family function scores of adolescents in the experimental group were decreased more as compared with the control group. ÖzetBu çalışmanın amacı, ergen-ebeveyn ilişki geliştirme programı’nın ergenlerin ebeveynlerinden algıladıkları red düzeylerine ve aile işlevlerine etkisini araştırmaktır. Araştırmanın deney ve kontrol grubu toplam 18 ergen ve ebeveyn çiftinden oluşmaktadır. Araştırmada Ebeveyn Kabul-Red Ölçeği (EKRÖ/Çocuk)ve Aile Değerlendirme Ölçeği (ADÖ) kullanılmıştır. Deney ve kontrol gruplarına bu ölçekler ön-test ve son-test olarak uygulanmıştır. Oturumlar yaklaşık 120 dakika olmak üzere 10 hafta sürmüştür. Kontrol grubu ile herhangi bir çalışma yapılmamıştır. Verilerin analizinde mixed (karışık) desenlerde kullanılması uygun bir teknik olan “Tekrarlanmış Ölçümler için 2x2 İki Faktörlü ANOVA” tekniği kullanılmıştır. Araştırma sonuçlarına göre, ergen ebeveyn ilişki geliştirme programı’na katılan ergenlerin (deney grubu) ebeveynlerinden algıladıkları red puanlarında, bu programa katılmayan ergenlerin (kontrol grubu) ebeveynlerinden algıladıkları red puanlarına göre anlamlı düzeyde bir azalma olmuştur. Ayrıca ergen ebeveyn ilişki geliştirme programı’na katılan ergenlerin (deney grubu) sağlıksız aile işlevi puanlarında, bu programa katılmayan ergenlerin (kontrol grubu) sağlıksız aile işlevi puanlarına göre anlamlı düzeyde bir azalma olmuştur.
PERENCANAAN KONSEP MINAPOLITAN DI KAWASAN TAMBAK LOROK, SEMARANG UTARA
Authors: Wulanningrum Sintia Dewi , Jayanti Theresia Budi 
Published: October 2016

<p>Minapolitan region is an economic region consisting of production centers, marine and fishery commodities, services, housing and other related activities. Tambak Lorok is one of fishing villages which is that located on the coastline of Java Sea in Tanjung Mas, North Semarang. The main function of Tambak Lorok is as residential areas, ponds and trades especially seafood. The main issues in Tambak Lorok are: the rob, low economy, and less infrastructure. The concept of minapolitan is used to improve the local economy of coastal communities through agribusiness subsystems and its supporting subsystems. This study employed descriptive qualitative method. </p><p> </p>
Tell Me Where You Are and I’ll Tell You What You Want: Using Location Data to Improve Marketing Decisions
Authors: Spann Martin , Molitor Dominik , Daurer Stephan 
Published: October 2016

Abstract
Location data has become more and more accessible. Smartphone applications such as location-based services collect location data on a large scale. Up to now, most approaches have relied on past data, but new developments in machine learning and artificial intelligence will soon enable more dynamic real-time use of location data. Companies that embrace these technologies will be able to create competitive advantages.
Location data offers great potential to improve a variety of marketing decisions such as targeted pricing and advertising, store locations and in-store layout. Location based advertising is currently the most common application. It allows targeting all customers within a certain distance of a store. Besides advertising, location data can be used for dynamic pricing decisions. Customers close to competitor’s locations can be charged a lower price for particular products via discounts in order to reduce switching costs. Indoor tracking can help to optimize store design or the positioning of categories and brands. Granular location data about consumers’ movements hence further allows for minimizing potential offline transaction costs based on the distances to stores.
The Art of Creating Attractive Consumer Experiences at the Right Time: Skills Marketers Will Need to Survive and Thrive
Authors: Lemon Katherine N. 
Published: October 2016

Abstract
New technologies have made today’s marketing faster, more mobile, more location-based, more digital, more virtual, and more automatized than ever. In this new world, marketers need to be “real-time relevant” – to gain awareness, to change perceptions and to spur action. They need to have their content in the right channel, format, time and context – from a consumer’s perspective. Only then do they at least have a chance of the consumer attending to the information and being influenced by it. In such an environment new skills and competences are required. The amount of available data has virtually exploded. To gain any perspective or apparent “control” in these environments, successful managers must embrace the complexity and learn to analyze, integrate and interpret all this data. A critical skill for marketers will be to identify the metrics that best reflect the desired outcomes of the organization and that sufficiently reflect specific indicators of critical processes. Furthermore, insights from other disciplines such as architecture, design, information-processing, biology or engineering will be important for creating customer experiences. The marketer of the future will need to be supremely curious and creative and to balance and integrate different worlds. It will all come down to delivering memorable and lasting experiences in a constantly and fast changing environment.
Big Data in Market Research: Why More Data Does Not Automatically Mean Better Information
Authors: Bosch Volker 
Published: October 2016

Abstract
Big data will change market research at its core in the long term because consumption of products and media can be logged electronically more and more, making it measurable on a large scale. Unfortunately, big data datasets are rarely representative, even if they are huge. Smart algorithms are needed to achieve high precision and prediction quality for digital and non-representative approaches. Also, big data can only be processed with complex and therefore error-prone software, which leads to measurement errors that need to be corrected. Another challenge is posed by missing but critical variables. The amount of data can indeed be overwhelming, but it often lacks important information. The missing observations can only be filled in by using statistical data imputation. This requires an additional data source with the additional variables, for example a panel. Linear imputation is a statistical procedure that is anything but trivial. It is an instrument to “transport information,” and the higher the observed data correlates with the data to be imputed, the better it works. It makes structures visible even if the depth of the data is limited.
Letter from the Editor in Chief
Authors: Levinson, MD Mark M. 
Published: October 2016

Twenty years ago, I presented a new vision for medical publishing. The Heart Surgery Forum was inaugurated in August of 1995 as a multimedia scientific publication communicating over the new “Information Highway” known as the graphical Web. Â In the early days of HTML and HTTP, new ideas could evolve and disseminate quickly to a community that spanned the globe. The HSF began as a “Labor of Love” for my profession and my colleagues as a dynamic tool for the betterment of themselves and their patients. Included in the original HSF Web site was a novel means to present interesting cases using color photos, movies, text and graphics, which was groundbreaking in 1995.
Sequence of the Sugar Pine Megagenome
Authors: Stevens Kristian A , Wegrzyn Jill L , Zimin Aleksey , Puiu Daniela , Crepeau Marc , Cardeno Charis , Paul Robin , Gonzalez-Ibeas Daniel , Koriabine Maxim , Holtz-Morris Ann E , Martínez-García Pedro J , Sezen Uzay U , Marçais Guillaume , Jermstad Kathy , McGuire Patrick E , Loopstra Carol A , Davis John M , Eckert Andrew , de Jong Pieter , Yorke James A , Salzberg Steven L , Neale David B , Langley Charles H 
Published: October 2016

AbstractUntil very recently, complete characterization of the megagenomes of conifers has remained elusive. The diploid genome of sugar pine (Pinus lambertiana Dougl.) has a highly repetitive, 31 billion bp genome. It is the largest genome sequenced and assembled to date, and the first from the subgenus Strobus, or white pines, a group that is notable for having the largest genomes among the pines. The genome represents a unique opportunity to investigate genome “obesity” in conifers and white pines. Comparative analysis of P. lambertiana and P. taeda L. reveals new insights on the conservation, age, and diversity of the highly abundant transposable elements, the primary factor determining genome size. Like most North American white pines, the principal pathogen of P. lambertiana is white pine blister rust (Cronartium ribicola J.C. Fischer ex Raben.). Identification of candidate genes for resistance to this pathogen is of great ecological importance. The genome sequence afforded us the opportunity to make substantial progress on locating the major dominant gene for simple resistance hypersensitive response, Cr1. We describe new markers and gene annotation that are both tightly linked to Cr1 in a mapping population, and associated with Cr1 in unrelated sugar pine individuals sampled throughout the species’ range, creating a solid foundation for future mapping. This genomic variation and annotated candidate genes characterized in our study of the Cr1 region are resources for future marker-assisted breeding efforts as well as for investigations of fundamental mechanisms of invasive disease and evolutionary response.
Degradation of the Mitotic Cyclin Clb3 Is not Required for Mitotic Exit but Is Necessary for G1 Cyclin Control of the Succeeding Cell Cycle
Authors: Pecani Kresti , Cross Frederick R 
Published: October 2016

Abstract
B-type cyclins promote mitotic entry and inhibit mitotic exit. In Saccharomyces cerevisiae, four B-type cyclins, Clb1–4, carry out essential mitotic roles, with substantial but incomplete overlap of function among them. Previous work in many organisms has indicated that B-type cyclin-dependent inhibition of mitotic exit imposes a requirement for mitotic destruction of B-type cyclins. For instance, precise genomic removal of the Clb2 destruction box (D box) prevents mitotic proteolysis of Clb2, and blocks mitotic exit. Here, we show that, despite significant functional overlap between Clb2 and Clb3, D-box-dependent Clb3 proteolysis is completely dispensable for mitotic exit. Removal of the Clb3 D box results in abundant Clb3 protein and associated kinase throughout the cell cycle, but mitotic exit occurs with close to normal timing. Clb3 degradation is required for pre-Start G1 control in the succeeding cell cycle. Deleting the CLB3 D box essentially eliminates all time delay before cell cycle Start following division, even in very small newborn cells. CLB3∆db cells show no cell cycle arrest response to mating pheromone, and CLB3∆db completely bypasses the requirement for CLN G1 cyclins, even in the absence of the early expressed B-type cyclins CLB5,6. Thus, regulated mitotic proteolysis of Clb3 is specifically required to make passage of Start in the succeeding cell cycle “memoryless”—dependent on conditions within that cycle, and independent of events such as B-type cyclin accumulation that occurred in the preceding cycle.
ВПРОВАДЖЕННЯ ІНФОРМАЦІЙНО–КОМУНІКАЦІЙНИХ ТЕХНОЛОГІЙ ПРИ ВИКЛАДАННІ ДИСЦИПЛІНИ «ВЕТЕРИНАРНА ТОКСИКОЛОГІЯ»
Authors: Boiko G.V. , Ischenko V.D. 
Published: October 2016

E–learning now becomes an actual part of the modern education system, provides a new opportunity in access to educational information resources, in the management of educational processes, in actualization of educational resources and management them, in the organizing of education new forms by using of distance learning, greatly enlarging possibilities for the traditional educational system. High efficiency of educational processes is achieved by collaborative used of e–learning different forms with traditional learning forms. This technology is called blended learning (blended–learning).The purpose of implementation the e–learning is to enhance the quality of education. The tasks to be solving directly by using the e–learning can be different and depend on the structure of educational institution and stage of development and other factors.Using of educational and information system has auxiliary character, and traditional teaching methods (including lectures) in this case saved. However, use of Moodle enables to move routine elements of discipline, which do not require serious consultations of teacher, in the educational system for individually study by students, and at lectures to focus more on problem issues of discipline.The article deals with the implementation of information and communication technologies in the Department of pharmacology and toxicology National University of Life and Environmental Sciences of Ukraine (Kyiv) at the teaching of "Veterinary toxicology" discipline by using the e–learning course created on the e–learning platform the Moodle and placed on the educational and information portal of the university.