bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disseminated toxoplasmosis after hematopoietic stem cell transplantation showing unusual magnetic resonance images
Authors: Tateno Takahiro , Onozawa Masahiro , Hashiguchi Junichi , Ishio Takashi , Yuzawa Sayaka , Matsuoka Satomi , Kosugi‐Kanaya Mizuha , Okada Kohei , Shiratori Souichi , Goto Hideki , Kimura Taichi , Sugita Junichi , Nakagawa Masao , Hashimoto Daigo , Kahata Kaoru , Fujimoto Katsuya , Endo Tomoyuki , Kondo Takeshi , Tanaka Shinya , Hashino Satoshi , Teshima Takanori 
Published: May 2017

AbstractWe herein report a patient who had disseminated toxoplasmosis after hematopoietic stem cell transplantation showing atypical clinical presentation and neuroimaging. Parkinsonism symptoms such as muscle rigidity, bradykinesia, tremor, and postural instability were initial manifestations. Magnetic resonance imaging showed diffuse symmetrical lesions of bilateral basal ganglia lacking ringed enhancement. Post‐mortem analysis revealed multiple tachyzoites of Toxoplasma gondii in the basal ganglia, mid brain, cerebellum, and cardiac muscle.
Mapping the human DC lineage through the integration of high-dimensional techniques
Authors: See Peter , Dutertre Charles-Antoine , Chen Jinmiao , Günther Patrick , McGovern Naomi , Irac Sergio Erdal , Gunawan Merry , Beyer Marc , Händler Kristian , Duan Kaibo , Sumatoh Hermi Rizal Bin , Ruffin Nicolas , Jouve Mabel , Gea-Mallorquí Ester , Hennekam Raoul C. M. , Lim Tony , Yip Chan Chung , Wen Ming , Malleret Benoit , Low Ivy , Shadan Nurhidaya Binte , Fen Charlene Foong Shu , Tay Alicia , Lum Josephine , Zolezzi Francesca , Larbi Anis , Poidinger Michael , Chan Jerry K. Y. , Chen Qingfeng , Rénia Laurent , Haniffa Muzlifah , Benaroch Philippe , Schlitzer Andreas , Schultze Joachim L. , Newell Evan W. , Ginhoux Florent 
Published: May 2017

Tracing development of the dendritic cell lineageDendritic cells (DCs) are important components of the immune system that form from the bone marrow into two major cell lineages: plasmacytoid DCs and conventional DCs. Seeet al.applied single-cell RNA sequencing and cytometry by time-of-flight to characterize the developmental pathways of these cells. They identified blood DC precursors that shared surface markers with plasmacytoid DCs but that were functionally distinct. This unsuspected level of complexity in pre-DC populations reveals additional cell types and refines understanding of known cell types.Science, this issue p.eaag3009
Draft Genome Sequences of the Aerobic Strains Lactobacillus gasseri AL3 and AL5
Authors: Maresca Diamante , De Filippis Francesca , Tytgat Hanne L. P. , de Vos Willem M. , Mauriello Gianluigi 
Published: May 2017

ABSTRACT

Adaptation to the aerobic environment has been investigated in heterofermentative lactobacilli, while data on how homofermentative lactobacilli adapt to oxygen are limited. We report here the draft genome sequences of the aerobic strains
Lactobacillus gasseri
AL3 and AL5 that allow an in-depth investigation of the genes involved in oxidative metabolism and the stress response.
Draft Genome Sequences of Two Isolates of
Colletotrichum lindemuthianum
, the Causal Agent of Anthracnose in Common Beans
Authors: de Queiroz Casley Borges , Correia Hilberty L. Nunes , Menicucci Renato Pedrozo , Vidigal Pedro M. Pereira , de Queiroz Marisa Vieira 
Published: May 2017

ABSTRACT

Colletotrichum lindemuthianum
is the causal agent of anthracnose in common beans, one of the main limiting factors of their culture. Here, we report for the first time, to our knowledge, a draft of the complete genome sequences of two isolates belonging to 83.501 and 89 A
2
2-3 of
C. lindemutuianum
.
Draft Genome Sequence of Clinical Strain TANI1 of Streptococcus suis Serotype 5 Isolated from a Bacteremia Patient in Japan
Authors: Yoshida Haruno , Wada Takayuki , Taniyama Daisuke , Takahashi Takashi 
Published: May 2017

ABSTRACT

Streptococcus suis
is a swine pathogen that causes severe economic damage to the porcine industry. It occasionally evokes zoonotic infection in humans. Here, we report a draft genome sequence of a
S. suis
serotype 5 strain isolated from a bacteremia patient that was reported by Taniyama et al. (D. Taniyama, M. Sakurai, T. Sakai, T. Kikuchi, and T. Takahashi, IDCases 6:36–38, 2016,
https://doi.org/10.1016/j.idcr.2016.09.011
).
Permanent Draft Genome Sequence of
Rhizobium
sp. Strain LCM 4573, a Salt-Tolerant, Nitrogen-Fixing Bacterium Isolated from Senegalese Soils
Authors: Diagne Nathalie , Swanson Erik , Pesce Céline , Fall Fatoumata , Diouf Fatou , Bakhoum Niokhor , Fall Dioumacor , Ndigue Faye Mathieu , Oshone Rediet , Simpson Stephen , Morris Krystalynne , Thomas W. Kelley , Moulin Lionel , Diouf Diegane , Tisa Louis S. 
Published: May 2017

ABSTRACT

The genus
Rhizobium
contains many species that are able to form nitrogen-fixing nodules on plants of the legume family. Here, we report the 5.5-Mb draft genome sequence of the salt-tolerant
Rhizobium
sp. strain LCM 4573, which has a G+C content of 61.2% and 5,356 candidate protein-encoding genes.
Draft Genome Sequences of Three Bacterial Isolates from Cultures of the Marine Diatom
Thalassiosira rotula
Authors: Garcia Nathan S. , Yung Cheuk-Man , Davis Katherine M. , Rynearson Tatiana , Hunt Dana E. 
Published: May 2017

ABSTRACT

Phytoplankton often both provision and depend on heterotrophic bacteria. In order to investigate these relationships further, we sequenced draft genomes of three bacterial isolates from cultures of the marine diatom
Thalassiosira rotula
to identify metabolic functions that may support interactions with
T. rotula
.
UK parents’ attitudes towards meningococcal group B (MenB) vaccination: a qualitative analysis
Authors: Jackson Cath , Yarwood Joanne , Saliba Vanessa , Bedford Helen 
Published: May 2017

Objectives(1) To explore existing knowledge of, and attitudes, to group B meningococcal disease and serogroup B meningococcal (MenB) vaccine among parents of young children. (2) To seek views on their information needs.DesignCross-sectional qualitative study using individual and group interviews conducted in February and March 2015, prior to the introduction of MenB vaccine (Bexsero) into the UK childhood immunisation schedule.SettingCommunity centres, mother and toddler groups, parents’ homes and workplaces in London and Yorkshire.Participants60 parents of children under 2 years of age recruited via mother and baby groups and via an advert posted to a midwife-led Facebook group.ResultsAlthough recognising the severity of meningitis and septicaemia, parents’ knowledge of group B meningococcal disease and MenB vaccine was poor. While nervous about fever, most said they would take their child for MenB vaccination despite its link to fever. Most parents had liquid paracetamol at home. Many were willing to administer it after MenB vaccination as a preventive measure, although some had concerns. There were mixed views on the acceptability of four vaccinations at the 12-month booster visit; some preferred one visit, while others favoured spreading the vaccines over two visits. Parents were clear on the information they required before attending the immunisation appointment.ConclusionsThe successful implementation of the MenB vaccination programme depends on its acceptance by parents. In view of parents’ recognition of the severity of meningitis and septicaemia, and successful introduction of other vaccines to prevent bacterial meningitis and septicaemia, the MenB vaccination programme is likely to be successful. However, the need for additional injections, the likelihood of post-immunisation fever and its management are issues about which parents will need information and reassurance from healthcare professionals. Public Health England has developed written information for parents, informed by these findings.
Patient and physiotherapist perceptions of rehabilitation following primary lumbar discectomy: a qualitative focus group study embedded within an external pilot and feasibility trial
Authors: Rushton Alison , Heneghan Nicola R , Heap Alison , White Louise , Calvert Melanie , Goodwin Peter C 
Published: May 2017

ObjectiveTo evaluate patients’ and physiotherapists’ perceptions, preferences and feelings about rehabilitation following lumbar discectomy surgery.DesignA qualitative focus group study, informed from the theoretical perspective of phenomenology, of patients’ and physiotherapists’ experiences of rehabilitation following lumbar discectomy was conducted. The focus groups were used to explore patients’ and physiotherapists’ perceptions and their preferences and feelings about different approaches to rehabilitation. The focus groups were facilitated and observed by experienced researchers and were informed by a topic guide that had been piloted previously.SettingThe study was embedded within an external pilot and feasibility trial that randomised patients across two secondary care spinal surgery sites in the UK to receive either 1:1 physiotherapy and leaflet or leaflet-only interventions.ParticipantsFive focus groups took place between April and July 2014. A framework analysis of thematic coding (deductive and inductive components) by two researchers captured identified themes common to both patients and physiotherapists. Data from three focus groups with patients and carers (n=11) and two with physiotherapists (n=15) contributed to the analytic framework.ResultsEmerging themes included: the value of patient leaflets with or without physiotherapy interventions; the importance of self-motivation in the recovery pathway; benefits of group physiotherapy for some patient groups and patient preference influencing rehabilitation.ConclusionPatients and physiotherapists perceived the study patient leaflet and 1:1 physiotherapy interventions as high quality and valuable. Patients’ personal priorities, for example, their need to return to work, influenced their preferences for rehabilitation interventions following surgery.
Resuscitation in hip fractures: a systematic review
Authors: Rocos Brett , Whitehouse Michael R , Kelly Michael B 
Published: May 2017

To evaluate the evidence for the resuscitation of patients with hip fracture in the preoperative or perioperative phase of their treatment and its impact on mortality.DesignWe searched MEDLINE, EMBASE, CENTRAL and PROSPERO databases using a systematic search strategy for randomised trials and observational studies investigating the fluid resuscitation of any patient with hip fracture. No language limits were applied to the search, which was complemented by manually screening the reference lists of appropriate studies.Outcome measuresMortality at 1 week, 30 days and 1 year following surgery.ResultsTwo hundred and ninety-eight citations were identified, and 12 full manuscripts were reviewed; no studies satisfied the inclusion criteria. The background literature showed that the mortality for these patients at 30 days is approximately 8.5% and that bone cement implantation syndrome is insufficient to explain this. The literature was explored to define the need for an interventional investigation into the preoperative resuscitation of patients with hip fracture.ConclusionsPatients with hip fracture show similar physiological disturbance to major trauma patients. Nineteen per cent of patients presenting with hip fracture are hypoperfused and 50% show preoperative anaemia suggesting that under resuscitation is a common problem that has not been investigated. A properly conducted interventional trial could improve the outcome of these vulnerable patients.
Effects of La2O3on the microstructure and tribological properties of plasma-sprayed Cr2O3–TiO2coatings
Authors: Ding Qingjun , Zhang Aihua , Zhao Gai , Peng Hanmin , Gao Wei 
Published: May 2017

Cr2O3–TiO2coatings with different proportions of La2O3were deposited by atmospheric plasma spraying. The coatings were evaluated by hardness tester, surface roughness tester, SEM and wear tester. The experiment results showed that the addition of La2O3could improve the microhardness and decrease porosity, wear rate and surface roughness of the coating. The coating containing 2 wt.% La2O3had the best tribological properties. The dominant wear mechanism is a mixture of abrasive wear and adhesive wear. The microscopic analysis suggests that the addition of La2O3could refine the microstructure and promote the formation of solid solution powder, and then affect the properties of coatings.
Oncogenic RAS Regulates Long Noncoding RNA Orilnc1 in Human Cancer
Authors: Zhang Dongmei , Zhang Gao , Hu Xiaowen , Wu Lawrence , Feng Yi , He Sidan , Zhang Youyou , Hu Zhongyi , Yang Lu , Tian Tian , Xu Weiting , Wei Zhi , Lu Yiling , Flaherty Keith T. , Zhong Xiaomin , Mills Gordon B. , Gimotty Phyllis A. , Xu Xiaowei , Herlyn Meenhard , Zhang Lin 
Published: May 2017

Abstract
RAS and its downstream cascades transmit cellular signals, resulting in increased transcription of genes involved in cell growth and division. Protein-coding gene targets of RAS signaling have been characterized extensively, but long noncoding RNAs (lncRNA) regulated by these processes have not. Using a custom-designed lncRNA microarray, we identified the lncRNA Orilnc1 as a genetic target of RAS that is critical for RAS oncogenicity. Orilnc1 expression was regulated by RAS–RAF–MEK–ERK signaling via the transcription factor AP1. Orilnc1 was highly expressed in BRAF-mutant cancers, such as melanoma. Silencing of Orilnc1 blocked tumor cell proliferation and growth in vitro and in vivo. In addition, Orilnc1 blockade reduced expression of cyclin E1 and induced G1–S cell-cycle arrest in tumor cells. Taken together, our results identify Orilnc1 as a novel, nonprotein mediator of RAS/RAF activation that may serve as a therapeutic target in RAS/RAF–driven cancers. Cancer Res; 77(14); 3745–57. ©2017 AACR.
Elacestrant (RAD1901), a Selective Estrogen Receptor Degrader (SERD), Has Antitumor Activity in Multiple ER+ Breast Cancer Patient-derived Xenograft Models
Authors: Bihani Teeru , Patel Hitisha K. , Arlt Heike , Tao Nianjun , Jiang Hai , Brown Jeffrey L. , Purandare Dinesh M. , Hattersley Gary , Garner Fiona 
Published: May 2017

Abstract
Purpose: Estrogen receptor–positive (ER+) breast cancers are typically treated with endocrine agents, and dependence on the ER pathway is often retained even after multiple rounds of antiestrogen therapy. Selective estrogen receptor degraders (SERD) are being developed as a strategy to more effectively target ER and exploit ER dependence in these cancers, which includes inhibiting both wild-type and mutant forms of ER. The purpose of this study was to evaluate the efficacy of a novel orally bioavailable SERD, elacestrant (RAD1901), in preclinical models of ER+ breast cancer.
Experimental Design: Elacestrant was evaluated as a single agent and in combination with palbociclib or everolimus in multiple ER+ breast cancer models, including several patient-derived xenograft models.
Results: Elacestrant induces the degradation of ER, inhibits ER-mediated signaling and growth of ER+ breast cancer cell lines in vitro and in vivo, and significantly inhibits tumor growth of multiple PDX models. Furthermore, we demonstrate that elacestrant in combination with palbociclib or everolimus can lead to greater efficacy in certain contexts. Finally, elacestrant exhibits significant antitumor activity both as a single agent and in combination with palbociclib in two patient-derived breast cancer xenograft models harboring ESR1 mutations.
Conclusions: These data underscore the potential clinical utility of elacestrant as a single agent and as a combination therapy, for both early- and late-stage ER+ disease. Clin Cancer Res; 23(16); 4793–804. ©2017 AACR.
Androgen Receptor Variant AR-V9 Is Coexpressed with AR-V7 in Prostate Cancer Metastases and Predicts Abiraterone Resistance
Authors: Kohli Manish , Ho Yeung , Hillman David W. , Van Etten Jamie L. , Henzler Christine , Yang Rendong , Sperger Jamie M. , Li Yingming , Tseng Elizabeth , Hon Ting , Clark Tyson , Tan Winston , Carlson Rachel E. , Wang Liguo , Sicotte Hugues , Thai Ho , Jimenez Rafael , Huang Haojie , Vedell Peter T. , Eckloff Bruce W. , Quevedo Jorge F. , Pitot Henry C. , Costello Brian A. , Jen Jin , Wieben Eric D. , Silverstein Kevin A.T. , Lang Joshua M. , Wang Liewei , Dehm Scott M. 
Published: May 2017

Abstract
Purpose: Androgen receptor (AR) variant AR-V7 is a ligand-independent transcription factor that promotes prostate cancer resistance to AR-targeted therapies. Accordingly, efforts are under way to develop strategies for monitoring and inhibiting AR-V7 in castration-resistant prostate cancer (CRPC). The purpose of this study was to understand whether other AR variants may be coexpressed with AR-V7 and promote resistance to AR-targeted therapies.
Experimental Design: We utilized complementary short- and long-read sequencing of intact AR mRNA isoforms to characterize AR expression in CRPC models. Coexpression of AR-V7 and AR-V9 mRNA in CRPC metastases and circulating tumor cells was assessed by RNA-seq and RT-PCR, respectively. Expression of AR-V9 protein in CRPC models was evaluated with polyclonal antisera. Multivariate analysis was performed to test whether AR variant mRNA expression in metastatic tissues was associated with a 12-week progression-free survival endpoint in a prospective clinical trial of 78 CRPC-stage patients initiating therapy with the androgen synthesis inhibitor, abiraterone acetate.
Results: AR-V9 was frequently coexpressed with AR-V7. Both AR variant species were found to share a common 3′ terminal cryptic exon, which rendered AR-V9 susceptible to experimental manipulations that were previously thought to target AR-V7 uniquely. AR-V9 promoted ligand-independent growth of prostate cancer cells. High AR-V9 mRNA expression in CRPC metastases was predictive of primary resistance to abiraterone acetate (HR = 4.0; 95% confidence interval, 1.31–12.2; P = 0.02).
Conclusions: AR-V9 may be an important component of therapeutic resistance in CRPC. Clin Cancer Res; 23(16); 4704–15. ©2017 AACR.
Curcumin Inhibits Heat-Induced Apoptosis by Suppressing NADPH Oxidase 2 and Activating the Akt/mTOR Signaling Pathway in Bronchial Epithelial Cells
Authors: Peng Yuan , Pu Junliang , Tang Chengyong , Wu Zhongjun 
Published: May 2017

Background: Heat causes bronchial epithelial cell apoptosis, which is a known factor contributing to airway damage during inhalation injury. Accumulating evidence has shown the effect of curcumin on inhibiting apoptosis. In this study, we investigated whether curcumin suppresses heat-induced apoptosis in bronchial epithelial cells and the underlying mechanism. Methods: Bronchial epithelial cell line 16HBE140 cells were incubated at either 42 °C, 47 °C, 52 °C, or 57 °C for 5 min in a cell incubator and then returned back to normal culture conditions (37 °C). An in vivo thermal inhalation injury rat model was established with a heat gun blowing hot air into the airway of rats. 16HBE140 cells and lung tissue were obtained for further study with or without curcumin treatment. Cell viability was determined by measuring the absorbance of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). 2',7'-dichlorofluorescein diacetate fluorescence was used as a measure of reactive oxygen species (ROS) production. Levels of Bcl2, Bax, α-ATP, cleaved Poly (ADP-ribose) polymerase (PARP), cleaved caspase-3, gp91phox, p47phox, p67phox, p22phox, p40phox, and Rac were determined by Western blotting. TUNEL staining was used to determine apoptosis. Results: Heat treatment triggered the apoptosis of 16HBE140 cells as shown by the increase in apoptosis molecular markers, including Bcl-2, Bax, cleaved PARP, and cleaved caspase-3. Administration of curcumin significantly inhibited apoptosis of 16HBE140 cells and suppressed the membrane translocation of NADPH oxidase 2 cytosolic components, as well as ROS production. Downregulation of Akt and mTOR phosphorylation induced by heat was also reversed by curcumin. Furthermore, we demonstrated that NADPH oxidase 2 is upstream of Akt/mTOR in heat-induced apoptosis. The protective role of curcumin on bronchial epithelia apoptosis was also confirmed in vivo by a rat inhalation injury model. Conclusion: This study demonstrates that one of the critical mechanisms underlying curcumin inhibiting heat-induced apoptosis is through suppressing NADPH Oxidase 2 and activating the Akt/mTOR signaling pathway in bronchial epithelial cells.
Apolipoprotein C-III Levels and Incident Coronary Artery Disease Risk
Authors: van Capelleveen Julian C. , Bernelot Moens Sophie J. , Yang Xiaohong , Kastelein John J.P. , Wareham Nicholas J. , Zwinderman Aeilko H. , Stroes Erik S.G. , Witztum Joseph L. , Hovingh G. Kees , Khaw Kay-Tee , Boekholdt S. Matthijs , Tsimikas Sotirios 
Published: May 2017

Objective—
Apolipoprotein C-III (apoC-III) is a key regulator of triglyceride metabolism. Elevated triglyceride-rich lipoproteins and apoC-III levels are causally linked to coronary artery disease (CAD) risk. The mechanism(s) through which apoC-III increases CAD risk remains largely unknown. The aim was to confirm the association between apoC-III plasma levels and CAD risk and to explore which lipoprotein subfractions contribute to this relationship between apoC-III and CAD risk.

Approach and Results—

Plasma apoC-III levels were measured in baseline samples from a nested case–control study in the European Prospective Investigation of Cancer (EPIC)-Norfolk study. The study comprised 2711 apparently healthy study participants, of whom 832 subsequently developed CAD. We studied the association of baseline apoC-III levels with incident CAD risk, lipoprotein subfractions measured by nuclear magnetic resonance spectroscopy and inflammatory biomarkers. ApoC-III levels were significantly associated with CAD risk (odds ratio, 1.91; 95% confidence interval, 1.48–2.48 for highest compared with lowest quintile), retaining significance after adjustment for traditional CAD risk factors (odds ratio, 1.47; 95% confidence interval, 1.11–1.94). ApoC-III levels were positively correlated with triglyceride levels, (
r
=0.39), particle numbers of very-low-density lipoprotein (
r
=0.25), intermediate-density lipoprotein (
r
=0.23), small dense low-density lipoprotein (
r
=0.26), and high-sensitivity C-reactive protein (
r
=0.15), whereas an inverse correlation was observed with large low-density lipoprotein particle number (
r
=−0.11),
P
<0.001 for each. Mediation analysis indicated that the association between apoC-III and CAD risk could be explained by triglyceride elevation (triglyceride, very-low-density lipoprotein, and intermediate-density lipoprotein particles), small low-density lipoprotein particle size, and high-sensitivity C-reactive protein.

Conclusions—
ApoC-III levels are significantly associated with incident CAD risk. Elevated levels of remnant lipoproteins, small dense low-density lipoprotein, and low-grade inflammation may explain this association.