bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the effect of omega-3 supplements and fresh fish on lipid profile: a randomized, open-labeled trial
Authors: Zibaeenezhad Mohammad Javad , Ghavipisheh Maryam , Attar Armin , Aslani Amir 
Published: December 2017

Abstract
Background
Dietary fish is a rich source of Omega-3 poly-unsaturated fatty acids (PUFAs). These compounds may have protective effect against cardiovascular events possibly by modifying lipid profiles. Consequently, fish oil supplements are produced commercially to complement low fish intake. It is not clear if both interventions have similar effects. The aim of this trial was to compare the anti-hyperlipidemic effect of omega3 fatty acid supplements with fresh fish.

Method
A total of 106 patients with hyperlipidemia were randomized. One group received 2 g/day of omega-3 capsules for a period of 8 weeks and the other group received a mean of 250 g trout fish twice weekly (for dinner and lunch) for the same time period. The effects of these diets on the lipid profile after the intervention were compared between the two groups.

Results
Data from 48 patients in fish oil group and 47 patients from fish group was used for final analysis. In both groups, total cholesterol, non-HDL cholesterol, triglyceride (TG) levels, and Castelli I index (total cholesterol/HDL ratio) were reduced significantly following the treatment; however, dietary-fish intake had a more pronounced effect (−85.08 ± 74.82 vs. −30.75 ± 89.00, P < 0.001; 75.06 ± 35.43 vs. −16.93 ± 40.21, P < 0.001; −66.55 ± 30.79 vs. −12.7 ± 35.48, P = 0.003; and −0.77 ± 1.39 vs. −3.02 ± 1.85, P < 0.001; respectively). HDL level was increased in both groups with a higher effect in dietary fish group (4.47 ± 7.83 vs. 8.51 ± 8.79, P = 0.022). Atherogenic (Log [TG/HDL ratio]) and Castelli II (LDL/HDL ratio) indices did not change in fish oil group while were reduced significantly by fresh fish consumption (−0.04 ± 0.27 vs. −0.26 ± 0.17, P < 0.001; and 0.15 ± 0.7 vs. -1.32 ± 1.15, P < 0.001, respectively). LDL level was increased in the supplementation group, while it was significantly reduced in the dietary-fish group (+18.7 ± 24.97 vs. −22.75 ± 27.28, P < 0.001).

Conclusion
Consumption of fresh fish seems to be superior in positively modifying the lipid profiles which may have important translations in the occurrence of cardiovascular events.
MicroRNA-105 is involved in TNF-α-related tumor microenvironment enhanced colorectal cancer progression
Authors: Shen Zetao , Zhou Rui , Liu Chen , Wang Yaofeng , Zhan Wanqi , Shao Ziyun , Liu Jian , Zhang Feifei , Xu Lijun , Zhou Xinying , Qi Lu , Bo Feng , Ding Yanqing , Zhao Liang 
Published: December 2017

AbstractTNF-α is a central proinflammatory cytokine contributing to malignant tumor progression in tumor microenvironment. In this study, we found the upregulation of miR-105 in colorectal cancer was associated with aggressive phenotype, and the enhanced expression of miR-105 was required for TNF-α-induced epithelial–mesenchymal transition (EMT). The expression of miR-105 was remarkably stimulated by TNF-α in a time-dependent manner using real-time qPCR analysis. Inhibition of miR-105 remarkably weakened the aggressive effects of TNF-α through preventing the activation of NF-κB signaling and the initiation of EMT. Furthermore, miR-105 was demonstrated directly targeted on the 3′-UTRs of RAP2C, a Rap2 subfamily of small GTP-binding protein. Consistently, suppression of RAP2C stimulated the role of miR-105, which dramatically promoted the invasion and metastasis of CRC cells. Thalidomide, a TNF-α and NF-κB inhibitor, significantly weakened the metastasis and homing capacity of miR-105-overexpressed CRC cells in nude mice. Our investigation initiatively illustrated the modulatory role of miR-105 in TNF-α-induced EMT and further CRC metastasis. We also offer a better understanding of TNFα-induced metastasis and suggest an effective therapeutic strategy against CRC metastasis.
The malignant niche: safe spaces for toxic stem cell marketing
Authors: Sipp Douglas 
Published: December 2017

AbstractMany tumors are sustained by microenvironments, or niches, that support and protect malignant cells, thus conferring a competitive advantage against both healthy cells and therapeutic interventions (for a brief review, see Yao and Link (Stem Cells 35: 3–8, 2017)). The global industry engaged in the commercial promotion of unproven and scientifically implausible cell-based “regenerative” therapies has developed a number of self-protective strategies that support its survival and growth in ways that are broadly analogous to the functions of the malignant niche.
VEGFR1 promotes cell migration and proliferation through PLCγ and PI3K pathways
Authors: Weddell Jared C. , Chen Si , Imoukhuede P. I. 
Published: December 2017

AbstractThe ability to control vascular endothelial growth factor (VEGF) signaling offers promising therapeutic potential for vascular diseases and cancer. Despite this promise, VEGF-targeted therapies are not clinically effective for many pathologies, such as breast cancer. VEGFR1 has recently emerged as a predictive biomarker for anti-VEGF efficacy, implying a functional VEGFR1 role beyond its classically defined decoy receptor status. Here we introduce a computational approach that accurately predicts cellular responses elicited via VEGFR1 signaling. Aligned with our model prediction, we show empirically that VEGFR1 promotes macrophage migration through PLCγ and PI3K pathways and promotes macrophage proliferation through a PLCγ pathway. These results provide new insight into the basic function of VEGFR1 signaling while offering a computational platform to quantify signaling of any receptor.
PECAplus: statistical analysis of time-dependent regulatory changes in dynamic single-omics and dual-omics experiments
Authors: Teo Guoshou , Bin Zhang Yun , Vogel Christine , Choi Hyungwon 
Published: December 2017

AbstractSimultaneous dynamic profiling of mRNA and protein expression is increasingly popular, and there is a critical need for algorithms to identify regulatory layers and time dependency of gene expression. A group of scientists from United States and Singapore present PECAplus, a comprehensive set of statistical analysis tools to address this challenge. Protein expression control analysis (PECA) computes the probability scores for change in mRNA and protein-level regulatory parameters at each time point, deconvoluting gene expression regulation in the presence of measurement noise. PECAplus adapted PECA’s mass action model to a variety of proteomic data including pulsed SILAC and generic protein expression data. It also features analysis modules to fit smooth curves on rugged time series observations, and to facilitate time-dependent interpretation of the data for genes and biological functions. They demonstrate the core modules with two time course datasets of mammalian cells responding to unfolded proteins and pathogens.
Complement enhances in vitro neutralizing potency of antibodies to human cytomegalovirus glycoprotein B (gB) and immune sera induced by gB/MF59 vaccination
Authors: Li Fengsheng , Freed Daniel C. , Tang Aimin , Rustandi Richard R. , Troutman Matthew C. , Espeseth Amy S. , Zhang Ningyan , An Zhiqiang , McVoy Michael , Zhu Hua , Ha Sha , Wang Dai , Adler Stuart P. , Fu Tong-Ming 
Published: December 2017

AbstractHuman cytomegalovirus (HCMV) is the leading cause of in utero viral infection in the United States. Since congenital HCMV infection can lead to birth defects in newborns, developing a prophylactic vaccine is a high priority. One of the early experimental vaccines, composed of a recombinant glycoprotein B (gB) formulated with MF59 adjuvant, has demonstrated approximately 50% efficacy against HCMV infection in seronegative women. Using immune sera from two gB/MF59 Phase 1 studies in humans we showed that complement can enhance the in vitro HCMV neutralizing potency of antibodies induced by the gB/MF59 vaccination. To characterize this complement-dependent antiviral activity, we analyzed three rabbit non-neutralizing gB monoclonal antibodies (mAbs) with different biochemical profiles including epitope specificity. Two of the three mAbs, r272.7 and r210.4, exhibited neutralizing activity when complement was added to the assays, and this complement-dependent antiviral activity was not related to the antibody’s affinity to gB but appeared to be associated with their epitope specificities. Moreover, neutralization could only be demonstrated when complement was present at or before viral entry, suggesting that IgG Fc-mediated function was not the basis for this antiviral activity. Lastly, we demonstrated that gB/MF59 immune sera contained antibodies that can cross-compete with r272.7 for gB binding and that the titers of these antibodies correlated with complement-dependent neutralization titers. These results suggested that gB antibodies with certain biochemical properties have neutralizing potency when complement is present and that this complement-dependent antiviral activity may be a part of immune components which conferred protection against HCMV infection by gB/MF59 vaccination.
Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL
Authors: Iida Keisuke , Sakai Ryo , Yokoyama Shota , Kobayashi Naritaka , Togo Shodai , Yoshikawa Hiroshi Y. , Rawangkan Anchalee , Namiki Kozue , Suganuma Masami 
Published: December 2017

AbstractTo study the role of cell softening in malignant progression, Transwell assay and atomic force microscope were used to classify six human non-small cell lung cancer cell lines into two groups: a high motility-low stiffness (HMLS) group and a low motility-high stiffness (LMHS) group. We found a significant role of activity of the AXL receptor tyrosine kinase, which belongs to the TAM (Tyro3, AXL, Mer) family, in the stimulation of motility and cell softening. HMLS cells expressed higher AXL levels than LMHS cells and contained phosphorylated AXL. H1703 LMHS cells transfected with exogenous AXL exhibited increased motility and decreased stiffness, with low levels of actin stress fibre formation. Conversely, the AXL-specific inhibitor R428 and AXL-targeting siRNA reduced motility and increased stiffness in H1299 HMLS cells. Knockdown of AXL stimulated actin stress fibre formation, which inhibited tumour formation in a mouse xenograft model. The Ras/Rac inhibitor SCH 51344, which blocks disruption of actin stress fibres, exerted similar effects to AXL inactivation. We therefore propose that the Ras/Rac pathway operates downstream of AXL. Thus, AXL activation-induced cell softening promotes malignant progression in non-small cell lung cancer and represents a key biophysical property of cancer cells.
Hubness of strategic planning and sociality influences depressive mood and anxiety in College Population
Authors: Yun Je-Yeon , Choi Yoobin , Kwon Yoonhee , Lee Hwa Young , Choi Soo-Hee , Jang Joon Hwan 
Published: December 2017

AbstractDepressive mood and anxiety can reduce cognitive performance. Conversely, the presence of a biased cognitive tendency may serve as a trigger for depressive mood-anxiety. Previous studies have largely focused on group-wise correlations between clinical-neurocognitive variables. Using network analyses for intra-individual covariance, we sought to decipher the most influential clinical-neurocognitive hub in the differential severity of depressive-anxiety symptoms in a college population. Ninety college students were evaluated for depressive-anxiety symptoms, Minnesota multiphasic personality inventory-2(MMPI-2), and neuro-cognition. Weighted and undirected version of the intra-individual covariance networks, comprised of 18 clinical-neurocognitive variables satisfied small-worldness and modular organization in the sparsity range ofK = 0.20–0.21. Furthermore, betweenness centrality of perseverative error for the Wisconsin card sorting test was reduced in more depressive individuals; higher anxiety was related to the increased betweenness centrality of MMPI-2 clinical scale 0(Si). Elevated edge-betweenness centrality of covariance between the MMPI-2 clinical scale 7(Pt) versus commission error of the continuous performance test predicted more anxiety higher than depressive mood. With intra-individual covariance network of clinical-neurocognitive variables, this study demonstrated critical drivers of depressive mood[attenuated influence of strategic planning] or anxiety[domination of social introversion/extroversion, in addition to the influence of compulsivity-impulsivity covariance as a shortcut component among various clinical-neurocognitive features].
Quantum-mechanical analysis of effect of alloying elements on ε-martensite start temperature of steels
Authors: Jang J. H. , Moon J. , Ha H.-Y. , Lee T.-H. , Suh D.-W. 
Published: December 2017

AbstractWith regard to the transformation mechanism of austenitic high manganese steel, the prediction of the ε-martensite start temperature is a critical consideration in alloy design. Evaluation of the ε-martensite start temperature makes it possible to predict the microstructure and to understand the phase transformation occurring during deformation. Here we use the quantum mechanical calculation of random alloys to understand the physics for ε-martensitic transformation in steels. We could find the linear relationship between the measured ε-martensite start temperatures and the crystal structure stability for various compositions. We also could estimate the effect of several alloying elements. It is expected that the effect of decreasing the temperatures for the same amount of alloying elements addition will be larger moving farther from Group VIII. By creating a free-energy model that reflects the temperature effect, we were able to calculate the average driving force required for the ε-martensitic transformations.
Nachwuchs in der Nervenheilkunde: berufswunschprägende Faktoren in Psychiatrie, Neurologie und Psychosomatik
Authors: Lange-Kasch Susanne , Wirkner Janine , Kasch Richard , Freyberger Harald , Fleßa Steffen , Merk Harry , Klauer Thomas 
Published: December 2017

Zusammenfassung
Ziel der Studie Studierende mit Spezialisierungspräferenz für eines von 3 nervenheilkundlichen Fächern wurden im Hinblick auf ihre Motivation zum Medizinstudium retrospektiv verglichen.
Methodik Mit dem Ziel der Identifikation früher Bedingungen von Spezialisierungspräferenzen wurde eine deutschlandweite Online-Befragung an 9079 Medizinstudierenden durchgeführt. Die Angaben der 669 Studierenden mit nervenheilkundlicher Präferenz wurden varianzanalytisch ausgewertet.
Ergebnisse Angehende Neurologen wurden vergleichsweise stärker von inhaltlichen Interessen am Fach Medizin und den Naturwissenschaften, weniger jedoch von Aspekten der Lebensführung motiviert. Hingegen spielten bei der Spezialisierungspräferenz für die psychologischen Fächer der Wunsch, aktiv Hilfe zu leisten, und die eigene Krankheitsanamnese eine wichtigere Rolle.
Schlussfolgerung Die aufgezeigten Motive zeigen thematische Richtungen auf, die bei der fachspezifischen Werbung um Nachwuchs verfolgt werden können.
Testdiagnostik in der PPmP
Authors: Brähler Elmar , Zenger Markus 
Published: December 2017

Bei der PPmP werden seit längerer Zeit sehr viele testdiagnostische Beiträge eingereicht. Ein Grund hierfür ist sicherlich, dass es für testdiagnostische deutschsprachige Manuskripte nur sehr wenige Zeitschriften gibt, die in Frage kommen. So gibt es als spezialisierte Zeitschrift eigentlich nur noch die Diagnostica. Die PPmP-Schriftleitung hat versucht, das Überangebot von testdiagnostischen Manuskripten zu kanalisieren, in dem sie eine Rubrik für testdiagnostische Verfahren eingerichtet hat. Dennoch werden außerhalb der Rubrik noch sehr viele Manuskripte angeboten, die sich mit testdiagnostischen Verfahren beschäftigen.Trotz aller Bedenken gegenüber einer Testlastigkeit unserer Zeitschrift gibt es durchaus gewichtige Gründe dafür, testdiagnostischen Manuskripten in der PPmP ausreichend Raum zu gewähren.
Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID
Authors: Ibrahim Elsayed , Gul Waseem , Gul Shahbaz , Stamper Brandon , Hadad Ghada , Abdel Salam Randa , Ibrahim Amany , Ahmed Safwat , Chandra Suman , Lata Hemant , Radwan Mohamed , ElSohly Mahmoud 
Published: December 2017

AbstractCannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 – 0.19 µg/mL, and the limit of quantitation was 0.34 – 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).
Evaluating the Effectiveness of Visual Narrative Illustrations Used to Teach Pathophysiology to Undergraduate Nursing Students
Authors: El Hussein Mohamed , Salyers Vincent , Osuji Joseph 
Published: December 2017

Abstract
Nursing students and nurses often struggle with understanding pathophysiology concepts. Seventy-five students participated in two phases of an exploratory study that evaluated knowledge of pathophysiology concepts when taught using an innovation known as “visual narrative illustrations” (VNIs). Students taught using VNIs performed significantly better on a knowledge posttest and commented that the VNI humor and visual images helped them learn complex concepts. Used in combination with other teaching strategies, VNIs enhanced knowledge and understanding of pathophysiology concepts.
Histone deacetylase 5 (HDAC5) regulates neuropathic pain through SRY-related HMG-box 10 (SOX10)-dependent mechanism in mice
Authors: Gu Pan , Pan Zhiqiang , Wang Xiao-Min , Sun Liting , Tai Lydia Wai , Cheung Chi Wai 
Published: December 2017

Abstract
A strong link between histone deacetylases (HDACs) and nociceptive hypersensitivity has been indicated in different pain models. However, the underlying molecular and cellular mechanisms remain elusive. Here, we discovered that partial sciatic nerve ligation–induced mechanical allodynia and thermal hyperalgesia in mice were associated with increased mRNA and protein expressions of HDAC5 (a member of class IIa HDACs) and SRY-related HMG-box 10 (SOX10) in the ipsilateral lumbar dorsal horn. Gene knockdown of spinal HDAC5 or SOX10 attenuated partial sciatic nerve ligation–induced nociceptive hypersensitivity, companied with decrease of spinal neuronal sensitization markers, namely phosphorylated-Erk, phosphorylated-GluN1 (ser896), and c-Fos. Conversely, overexpression of spinal HDAC5 or SOX10 by lentiviruses in naive mice not only induced pain-like behaviors but also increased the expression of these spinal neuronal sensitization markers. Of note, in contrast to its conventional deacetylation effect to silence gene expression, overexpression of HDAC5 not only enhanced SOX10 expression but also induced nociceptive hypersensitivity in naive mice, which were reversed by SOX10 gene knockdown. Chromatin-immunoprecipitation assay further confirmed a novel nonhistone modulation function of HDACs on SOX10 expression, that is, HDAC5 regulates SOX10 by binding to the promoter region of Sox10 gene. In conclusion, this study for the first time demonstrates that HDAC5 regulates spinal neuronal sensitization in neuropathic pain by upregulating modulating SOX10 expression. Thus, interventions that reduce HDAC5/SOX10 expression may represent promising avenues in the treatment of neuropathic pain.
Anti-Neutrophil Cytoplasmic Antibody-Negative Central Nervous System Granulomatosis With Polyangiitis and Its Clinical Characteristics
Authors: Chen Zhihua , Miao Yifeng , Wu Hui , Wang Ran , Zhou Zhiyi , Zhang Shilei , Chen Longtian , Qiu Yongming 
Published: December 2017

Abstract
Granulomatosis with polyangiitis (GPA) is a necrotizing granulomatous vasculitis occasionally affecting central nervous system (CNS), and GPA patients with initial CNS symptoms are even rarer, whose diagnosis is further confused by an absence of positive antineutrophil cytoplasmic antibody. The authors described the characteristics of antineutrophil cytoplasmic antibody -negative GPA with CNS onset in a patient and discussed on its management, which may contribute to future diagnosis and treatment of patients with similar conditions.
Efficacy of a Dual-ring Wound Protector for Prevention of Surgical Site Infections After Pancreaticoduodenectomy in Patients With Intrabiliary Stents
Authors: Bressan Alexsander K. , Aubin Jean-Michel , Martel Guillaume , Dixon Elijah , Bathe Oliver F. , Sutherland Francis R. , Balaa Fady , Mimeault Richard , Edwards Janet P. , Grondin Sean C. , Isherwood Susan , Lillemoe Keith D. , Saeed Sara , Ball Chad G. 
Published: December 2017

Objective:
To evaluate the efficacy of a dual-ring wound protector for preventing incisional surgical site infection (SSI) among patients with preoperative biliary stents undergoing pancreaticoduodenectomy (PD).

Methods and analysis:
This study was a parallel, dual-arm, double-blind randomized controlled trial. Adult patients with a biliary stent undergoing elective PD at 2 tertiary care institutions were included (February 2013 to May 2016). Patients were randomly assigned to receive a surgical dual-ring wound protector or no wound protector, and also the current standard of care. The main outcome measure was incisional SSI, as defined by the Centers for Disease Control and Prevention criteria, within 30 days of the index operation.

Results:
A total of 107 patients were recruited (mean age 67.2 years; standard deviation 12.9; 65% male). No significant differences were identified between the intervention and control groups (age, sex, body mass index, preoperative comorbidities, American Society of Anesthesiologists class, prestent cholangitis). There was a significant reduction in the incidence of incisional SSI in the wound protector group (21.1% vs 44.0%; relative risk reduction 52%; P = 0.010). Patients with completed PD also displayed a decrease in incisional SSI with use of the wound protector compared with those palliated surgically (27.3% vs 48.7%; P = 0.04). Multivariate analysis did not identify any significant modifying factor relationships (estimated blood loss, duration of surgery, hospital site, etc.) (P > 0.05).

Conclusion:
Among adult patients with intrabiliary stents, the use of a dual-ring wound protector during PD significantly reduces the risk of incisional SSI.