bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sniffer mice discriminate urine odours of patients with bladder cancer: A proof-of-principle study for non-invasive diagnosis of cancer-induced odours
Authors: Sato Takaaki , Katsuoka Yoji , Yoneda Kimihiko , Nonomura Mitsuo , Uchimoto Shinya , Kobayakawa Reiko , Kobayakawa Ko , Mizutani Yoichi 
Published: November 2017

AbstractSimilar to fingerprints, humans have unique, genetically determined body odours. In case of urine, the odour can change due to variations in diet as well as upon infection or tumour formation. We investigated the use of mice in a manner similar to “sniffer dogs” to detect changes in urine odour in patients with bladder cancer. We measured the odour discrimination thresholds of mice in a Y-maze, using urine mixtures from patients with bladder cancer (Stage I) and healthy volunteers (dietary variations) as well as occult blood- or antibiotic drug metabolite-modulated samples. Threshold difference indicated that intensities of urinary olfactory cues increase in the following order: dietary variation < bladder cancer < occult blood < antibiotic drug metabolites. After training with patient urine mixtures, sniffer mice discriminated between urine odours of pre- and post-transurethral resection in individual patients with bladder cancer in an equal-occult blood diluted condition below the detection level of dietary variations, achieving a success rate of 100% (11/11). Furthermore, genetic ablation of all dorsal olfactory receptors elevated the discrimination thresholds of mice by ≥ 105-fold. The marked reduction in discrimination sensitivity indicates an essential role of the dorsal olfactory receptors in the recognition of urinary body odours in mice.
Optimization reconstruction of projective point of laser line coordinated by orthogonal reference
Authors: Xu Guan , Yuan Jing , Li Xiaotao , Su Jian 
Published: November 2017

AbstractA 3D reconstruction method is presented for the laser projective point of a laser line, which is located by an orthogonal reference. The laser line is initially expressed by the Plücker matrix generated from two random points on the line and then transformed to the dual Plücker matrix representation. The initial solution of the 3D laser point is obtained by the non-homogeneous linear equations, which are derived from the projection geometry of the 3D feature point on the reference and the 3D laser point on the laser line represented by the dual Plücker matrix. The optimization function is constructed by minimizing the sums of the re-projection errors of the reference points and the laser point. The average absolute error of the initial solution is 1.07 mm while the one of the optimization solution is 1.01 mm. The average relative error of the initial solution is 4.14% while the one of the optimization solution is 3.86%. Thus, the optimization reconstruction of the projective point contributes the accuracy and the prospect in the vision-based inspection fields.
The performance and archaeal community shifts in a modified anaerobic baffled reactor treating sweet potato starch wastewater at ambient temperatures
Authors: Xu Shengjun , Jiang Cancan , Ma Shuanglong , Wu Shanghua , Bai Zhihui , Zhuang Guoqiang , Zhuang Xuliang 
Published: November 2017

AbstractA conventional anaerobic baffled reactors (ABRs) treating high strength sweet potato starch wastewater at ambient temperatures resulted in acidification and bad performances. After modification, the acidification was remitted and COD removal efficiencies reached 92.73% at high temperatures and were maintained at 71.19% at low temperatures. Moreover, as much as 1.014 ± 0.056 L CH4/L/d were collected at Stage III. The q-PCR results revealed that the largest methanogen populations emerged at Stage III as well, which was 5.29 × 108 mcrA copies per milliliter sludge. A comparable shift in the archaeal community structure at different stages and acetoclastic methanogens Methanosaeta predominated the archaeal community in every compartment in Stages I (63.73%) and II (48.63%). Finally, the net energy gains analysis at mesophilic, thermophilic, and ambient temperature revealed that modified ABR at ambient temperature was not only economical but also profitable and could generated 3.68 KJ energy per gram COD removed.
Over-expression of ΔNp63α facilitates rat corneal wound healingin vivo
Authors: Yao Jeng-Yuan , Chen Jan-Kan 
Published: November 2017

AbstractTo investigate the roles of ΔNp63α during corneal wound healing and the genes regulated by ΔNp63α in limbal epithelial cells. Adenovirus or shRNA targeting ΔNp63α were pre-injected into the anterior chamber of rat eyeballs and the central corneal epithelium was then wounded with NaOH. The effects of ΔNp63α expression during wound healing were observed by propidium iodide staining. In addition, limbal epithelial cells were cultured and ectopically expressed ΔNp63α by transfecting Ad-ΔNp63α. Total RNA was extracted from transfected epithelial cells and subjected to a gene expression microarray assay. The results showed that over-expression of ΔNp63α accelerated the process of corneal wound healing while knockdown of ΔNp63α impaired the process. ΔNp63α positively up-regulated several cell growth promoter genes and could be referred as a positive regulator of limbal epithelial cell proliferation. It might also inhibit cell differentiation and cell death by differential target gene regulation.
Structure and function of polyketide biosynthetic enzymes: various strategies for production of structurally diverse polyketides
Authors: Miyanaga Akimasa 
Published: November 2017

Abstract
Polyketides constitute a large family of natural products that display various biological activities. Polyketides exhibit a high degree of structural diversity, although they are synthesized from simple acyl building blocks. Recent biochemical and structural studies provide a better understanding of the biosynthetic logic of polyketide diversity. This review highlights the biosynthetic mechanisms of structurally unique polyketides, β-amino acid-containing macrolactams, enterocin, and phenolic lipids. Functional and structural studies of macrolactam biosynthetic enzymes have revealed the unique biosynthetic machinery used for selective incorporation of a rare β-amino acid starter unit into the polyketide skeleton. Biochemical and structural studies of cyclization enzymes involved in the biosynthesis of enterocin and phenolic lipids provide mechanistic insights into how these enzymes diversify the carbon skeletons of their products.
Biomass Briquette Investigation from Pterocarpus Indicus Leaves Waste as an Alternative Renewable Energy
Authors: Anggono Willyanto , Sutrisno  , Suprianto Fandi D. , Evander Jovian 
Published: November 2017

Abstract
Indonesia is a tropical country located in Southeast Asia. Indonesia has a lot of variety of plant species which are very useful for life. Pterocarpus indicus are commonly used as greening and easily found everywhere in Surabaya city because of its characteristics that they have dense leaves and rapid growth. Pterocarpus indicus leaves waste would be a problem for residents of Surabaya and disturbing the cleanliness of the Surabaya city. Therefore, the Pterocarpus indicus leaves waste would be used as biomass briquettes. This research investigated the calorific value of biomass briquettes from the Pterocarpus indicus leaves waste, the effect of tapioca as an adhesive material to the calorific value of biomass briquettes from the Pterocarpus indicus leaves waste, the optimum composition for Pterocarpus indicus leaves waste biomass briquette as an alternative renewable fuel and the property of the optimum resulted biomass briquette using ultimate analysis and proximate analysis based on the ASTM standard. The calorific value biomass briquettes from the Pterocarpus indicus leaves waste were performed using an oxygen bomb calorimeter at various composition of Pterocarpus indicus from 50% to 90% rising by 10% for each experiment. The experimental results showed that the 90% raw materials (Pterocarpus indicus leaves waste)-10% adhesive materials (tapioca) mixtures is the optimum composition for biomass briquette Pterocarpus indicus leaves waste. The lower the percentage of the mass of tapioca in the biomass briquettes, the higher calorific value generated.
Phosphorylation of multiple sites within an acidic region of Alcadein α is required for kinesin-1 association and Golgi exit of Alcadein α cargo
Authors: Sobu Yuriko , Furukori Keiko , Chiba Kyoko , Nairn Angus C. , Kinjo Masataka , Hata Saori , Suzuki Toshiharu 
Published: November 2017

Alcadein α (Alcα) is a major cargo of kinesin-1 that is subjected to anterograde transport in neuronal axons. Two tryptophan- and aspartic acid-containing (WD) motifs located in its cytoplasmic domain directly bind the tetratricopeptide repeat (TPR) motifs of the kinesin light chain (KLC), which activate kinesin-1 and recruit kinesin-1 to Alcα cargo. We found that phosphorylation of three serine residues in the acidic region located between the two WD motifs is required for interaction with KLC. Phosphorylation of these serine residues may alter the disordered structure of the acidic region to induce direct association with KLC. Replacement of these serines with Ala results in a mutant that is unable to bind kinesin-1, which impairs exit of Alcα cargo from the Golgi. Despite this deficiency, the compromised Alcα mutant was still transported, albeit improperly by vesicles following missorting of the Alcα mutant with amyloid β-protein precursor (APP) cargo. This suggests that APP partially compensates for defective Alcα in anterograde transport by providing an alternative cargo receptor for kinesin-1.
pLoc-mHum: predict subcellular localization of multi-location human proteins via general PseAAC to winnow out the crucial GO information
Authors: Cheng Xiang , Xiao Xuan , Chou Kuo-Chen 
Published: November 2017

Abstract

Motivation
For in-depth understanding the functions of proteins in a cell, the knowledge of their subcellular localization is indispensable. The current study is focused on human protein subcellular location prediction based on the sequence information alone. Although considerable efforts have been made in this regard, the problem is far from being solved yet. Most existing methods can be used to deal with single-location proteins only. Actually, proteins with multi-locations may have some special biological functions that are particularly important for both basic research and drug design.

Results
Using the multi-label theory, we present a new predictor called ‘pLoc-mHum’ by extracting the crucial GO (Gene Ontology) information into the general PseAAC (Pseudo Amino Acid Composition). Rigorous cross-validations on a same stringent benchmark dataset have indicated that the proposed pLoc-mHum predictor is remarkably superior to iLoc-Hum, the state-of-the-art method in predicting the human protein subcellular localization.

Availability and implementation
To maximize the convenience of most experimental scientists, a user-friendly web-server for the new predictor has been established at http://www.jci-bioinfo.cn/pLoc-mHum/, by which users can easily get their desired results without the need to go through the complicated mathematics involved.

Supplementary information
Supplementary data are available at Bioinformatics online.
Creating a ‘Suspect Community’: Monitoring and Controlling the Cypriot Community in Inter-War London
Authors: Smith Evan , Varnava Andrekos 
Published: November 2017

Abstract
This article offers a critical assessment of British policing and government monitoring and controlling of a ‘suspect community’ in inter-war London, which was also the subject of emigration control, the only colonial group to be thus treated prior to the Commonwealth Immigration Act 1962. The case is that of the Cypriots, who began to emigrate to the imperial metropolis in significant numbers in the late 1920s and largely settled in and around Soho. Their concentrated geographical location made them easy to police, monitor and control once their criminality and deviant communist politics made them a ‘suspect community’. They were also subjected to immigration control at their homeland by the Cypriot Colonial Government. There was significant co-operation between the Home and Colonial Offices to achieve the controls in both London and Cyprus. The immigration controls served as a prototype for the subsequent restrictions on other groups after the Second World War (namely those from India and Pakistan) and formed the basis of the Commonwealth Immigration Act.
CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis elegans
Authors: Cai Huaihan , Dhondt Ineke , Vandemeulebroucke Lieselot , Vlaeminck Caroline , Rasulova Madina , Braeckman Bart P 
Published: November 2017

Abstract
When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span–doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals’ food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.
Open-Access Physical Activity Programs for Older Adults: A Pragmatic and Systematic Review
Authors: Balis Laura E , Strayer Thomas , Ramalingam NithyaPriya , Wilson Meghan , Harden Samantha M 
Published: November 2017

AbstractBackground and ObjectivesOpen-access, community-based programs are recommended to assist older adults in meeting physical activity guidelines, but the characteristics, impact, and scalability of these programs is less understood. The Land-Grant University Cooperative Extension System, an organization providing education through county-based educators, functions as a delivery system for these programs. A systematic review was conducted to determine characteristics of effective older adult physical activity programs and the extent to which programs delivered in Extension employ these characteristics.Research Design and MethodsA systematic review of peer-reviewed and grey literature was conducted from August 2016 to February 2017. The review was limited to open-access (available to all), community-based physical activity interventions for older adults (≥65 years of age). The peer-reviewed literature search was conducted in PubMed and EBSCOhost; the grey literature search for Extension interventions was conducted through Extension websites, Land-Grant Impacts, and the Journal of Extension.ResultsSixteen peer-reviewed studies and 17 grey literature sources met inclusion criteria and were analyzed. Peer-reviewed and Extension programs were similar in their limited use of behavioral theories and group-based strategies. Compared to Extension programs, those in the peer-reviewed literature were more likely to use a combination of physical activity components and be delivered by trained professionals.Discussion and ImplicationsThe results indicate notable differences between peer-reviewed literature and Extension programs and present an opportunity for Extension programs to more effectively use evidence-based program characteristics, including behavioral theories and group dynamics, a combination of physical activity components, and educator/agent-trained delivery agents.
Draft genome of the reindeer (Rangifer tarandus)
Authors: Li Zhipeng , Lin Zeshan , Ba Hengxing , Chen Lei , Yang Yongzhi , Wang Kun , Qiu Qiang , Wang Wen , Li Guangyu 
Published: November 2017

Abstract

Background
The reindeer (Rangifer tarandus) is the only fully domesticated species in the Cervidae family, and it is the only cervid with a circumpolar distribution. Unlike all other cervids, female reindeer, as well as males, regularly grow cranial appendages (antlers, the defining characteristics of cervids). Moreover, reindeer milk contains more protein and less lactose than bovids' milk. A high-quality reference genome of this species will assist efforts to elucidate these and other important features in the reindeer.

Findings
We obtained 615 Gb (Gigabase) of usable sequences by filtering the low-quality reads of the raw data generated from the Illumina Hiseq 4000 platform, and a 2.64-Gb final assembly, representing 95.7% of the estimated genome (2.76 Gb according to k-mer analysis), including 92.6% of expected genes according to BUSCO analysis. The contig N50 and scaffold N50 sizes were 89.7 kilo base (kb) and 0.94 mega base (Mb), respectively. We annotated 21 555 protein-coding genes and 1.07 Gb of repetitive sequences by de novo and homology-based prediction. Homology-based searches detected 159 rRNA, 547 miRNA, 1339 snRNA, and 863 tRNA sequences in the genome of R. tarandus. The divergence time between R. tarandus and ancestors of Bos taurus and Capra hircus is estimated to be about 29.5 million years ago.

Conclusions
Our results provide the first high-quality reference genome for the reindeer and a valuable resource for studying the evolution, domestication, and other unusual characteristics of the reindeer.
A pro-inflammatory role of Fcα/μR on marginal zone B cells in sepsis
Authors: Shibuya Akira , Honda Shin-ichiro , Shibuya Kazuko 
Published: November 2017

AbstractFc receptors play important roles for a wide array of immune responses. In contrast to the well-defined Fcγ and Fcε receptors, the molecular and functional characteristics of Fc receptors for IgA and IgM have remained incompletely understood for years. Recent progress has unveiled the characteristics of Fc receptors for IgA and IgM, including Fcα/μ receptor (Fcα/μR) (CD351), polymeric immunoglobulin receptor (poly-IgR), Fcα receptor (FcαRI) (CD89) and Fcμ receptor (FcμR). In this review, we summarize the molecular and functional characteristics of Fcα/μR in comparison with poly-IgR, FcμR and FcαRI, and focus particularly on the pro-inflammatory function of Fcα/μR expressed on marginal zone B cells in sepsis.
Processing of intended and unintended strategic issues and integration into the strategic agenda
Authors: Ridder Hans-Gerd , Schrader Jan Simon 
Published: November 2017

Background:
Strategic change is needed in hospitals due to external and internal pressures. However, research on strategic change, as a combination of management and medical expertise in hospitals, remains scarce.

Purpose:
We analyze how intended strategic issues are processed into deliberate strategies and how unintended strategic issues are processed into emergent strategies in the management of strategy formation in hospitals. This study empirically investigates the integration of medical and management expertise in strategy formation.

Methodology:
The longitudinal character of the case study enabled us to track patterns of intended and unintended strategic issues over 2 years. We triangulated data from interviews, observations, and documents. In accordance with the quality standards of qualitative research procedures, we analyzed the data by pattern matching and provided analytical generalization regarding strategy formation in hospitals.

Results:
Our findings suggest that strategic issues are particularly successful within the strategy formation process if interest groups are concerned with the strategic issue, prospective profits are estimated, and relevant decisions makers are involved early on. Structure and interaction processes require clear criteria and transparent procedures for effective strategy formation.

Conclusion:
There is systematic neglect of medical expertise in processes of generating strategies.

Practice Implications:
Our study reveals that the decentralized structure of medical centers is an adequate template for both the operationalization of intended strategic issues and the development of unintended strategic issues. However, tasks, roles, responsibility, resources, and administrative support are necessary for effective management of strategy formation. Similarly, criteria, procedures, and decision-making are prerequisites for effective strategy formation.
Double-Docking Technique for Femtosecond Laser-Assisted Deep Anterior Lamellar Keratoplasty
Authors: Guindolet Damien , Nguyen Diem T. , Bergin Ciara , Doan Serge , Cochereau Isabelle , Gabison Eric E. 
Published: November 2017

Purpose:
To describe a new and safe surgical technique of deep anterior lamellar keratoplasty (DALK) using the femtosecond laser (FSL) and intraoperative optical coherence tomography (iOCT) for surgical management of corneal thinning and/or opacities. The technique was coined the double-docking procedure for DALK (DD-DALK).

Methods:
FSL-integrated iOCT was used for direct visualization and calibration to perform precise anterior lamellar and side cuts for the removal of the anterior stroma. Air was then injected in the residual posterior stroma to detach Descemet membrane [big-bubble (BB) formation]. Returning the residual posterior stroma into the docked position, a cylindrical vertical cut was made with the FSL to securely open the BB roof. Next, the stromal roof of the BB was removed with forceps leaving Descemet membrane intact, followed by a lamellar corneal graft.

Results:
Anterior stroma resection, BB formation, and residual stromal resection were achieved in every case without perforation. The curved applanation surface helped to limit the formation of folds on the posterior stroma (ie, advanced thinning).

Conclusions:
DD-DALK is a reproducible and safe procedure for surgical management of corneal thinning and/or opacities. The precision of stromal cuts made by the FSL and iOCT guidance for air injection increases success in DD-DALK preparation.