bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Drying Temperature on Agave tequilana Leaves: A Pretreatment for Releasing Reducing Sugars for Biofuel Production
Authors: Avila‐Gaxiola Evangelina , Avila‐Gaxiola Jorge , Velarde‐Escobar Oscar , Ramos‐Brito Francisco , Atondo‐Rubio Gelacio , Yee‐Rendon Cristo 
Published: July 2016

AbstractThe leaves of Agave tequilana Weber variety Blue represent a viable, inexpensive, and renewable source of lignocellulosic biomass and fructans for the production of second generation biofuels. The objective was to study the effect of drying temperature on the release of reducing sugars for the agave leaves. It was found that with pretreatment‐drying at 100°C for 30.5 ± 1.0 min had a maximum of the release of reducing sugars with a 66% increment compared to 60°C. An aqueous extract obtained from the powder of the leaves after drying did not show the presence of furfural and hydroxymethylfurfural compounds. Phenolic compounds were detected in order of 120.8 ± 1.0 mg L−1 below 1 g L−1 reported to cause inhibition of the alcoholic fermentation. In addition the drying of the leaves also can be used as preservation of agave leaf for biomass storing. The results show that pretreatment‐drying allow increase the release of reducing sugars, avoids thermal degradation and does not produce significant concentrations of fermentation inhibitors.Practical ApplicationsThe research in industrial waste materials has received special attention worldwide because it is potential for production of biofuels. This study propose a simple method of drying that acts as a thermal pretreatment of Agave tequilana Weber variety Blue leaves that increases the release of reducing sugars, avoids thermal degradation, not produce significant concentrations of fermentation inhibitors for ethanol production and can be used as a preservation method for storing biomass of the agave leaves. The results would be useful not only in the energy field but also in the alimentary and pharmaceutical industry. Fructans and phenolic compounds found in the agave are used as functional ingredients in the food industry and also bioactive compounds that by themselves promotes future research for the Agave tequilana.
Men at risk for paradoxical adipose hyperplasia after cryolipolysis
Authors: Keaney Terrence C , Naga Lina I 
Published: July 2016

SummaryCryolipolysis, an aesthetic procedure that reduces adipose tissue by exposure to cold temperature, is generally well tolerated with mild side effects including temporary numbness, erythema, and tenderness. However, as cryolipolysis is gaining popularity and more treatments are being performed, reports of rare adverse events including delayed onset pain and paradoxical adipose hyperplasia (PAH) have been described. Recent studies have suggested that PAH can be more common than expected and have a predilection for males, as a disproportionate number of the cases reported in the literature have occurred in men despite the fact that fewer men are likely to be treated with cryolipolysis. Sexual dimorphism in adipose anatomy may provide insight into the increased susceptibility of men to PAH. Careful patient selection avoiding men with visceral abdominal adipose and firm, nondistensible, fibrous fat may be important to minimize the risk of PAH.
Increased early inflammatory response and osteoclastic activity in gingival tissues following conventional osseous resective surgery compared with the fibre retention technique: a pilot study
Authors: Romano F. , Graziano A. , Spina A. , Ercoli E. , Audagna M. , Mariani G. M. , Ferrarotti F. , Aimetti M. 
Published: July 2016

Background and ObjectiveThe retention of suprabony connective fibres attached into the root cementum during fibre retention osseous resective surgery (FibReORS) results in a more conservative intrasurgical removal of bone, and limits further bone loss and patient morbidity during healing, compared with conventional osseous resective surgery (ORS). This may be a result of the protective effect of preserved connective tissue over the interproximal sites and the lower activation of the inflammatory mechanisms. Thus, the aim of this pilot study was to compare the expression of inflammatory and osteoclastic activity markers in gingival tissues following FibReORS and ORS in the early postsurgical phase.Material and MethodsTwenty‐six posterior sextants requiring osseous resective surgery were selected in 13 patients with chronic periodontitis: 13 sextants were randomly assigned to ORS and 13 to FibReORS in a split‐mouth design. Gingival biospies were collected during the surgical sessions and at suture removal. Tissue samples were analysed to evaluate the expression of proinflammatory and immunity regulatory mediators (interleukin‐1α, C‐X‐C motif chemokine ligand 5, interferon‐γ and tumour necrosis factor‐α), cluster of differentiation 14 (CD14; a monocyte/macrophage marker) and TRAP (an osteoclast marker) using immunohistochemical, immunofluorescence and cytofluorimetric analyses, respectively.ResultsPostsurgery, a higher number of inflammatory cells and stronger expression of proinflammatory cytokines were observed in the epithelium and connective tissue of ORS gingival samples compared with FibReORS gingival samples (p < 0.001). This was accompanied by increased numbers of CD14‐positive and TRAP‐positive cells.ConclusionRetention of the supracrestal connective fibres appears to reduce the postsurgical intensity of the host‐mediated inflammatory response.
Baiting for carnivores might negatively affect capture rates of prey species in camera‐trap studies
Authors: Rocha D. G. , Ramalho E. E. , Magnusson W. E. 
Published: July 2016

AbstractSurveying and monitoring of elusive animals with naturally low densities and large home ranges, such as many medium‐ and large‐sized mammals, is challenging. Low capture rates can preclude detailed analyses. The use of bait has been used as a strategy to increase carnivore capture rates in many camera‐trap surveys. Here, we test the effect of one carnivore bait type (mix of fresh sardine and egg) on the capture rates of carnivores and prey species in a camera‐trap survey in the Central Brazilian Amazon. We also test if the quality of records of naturally marked felids for individual identification is enhanced by the use of bait. We found that the bait had no apparent effect on the carnivore capture rates, but it might have repelled some prey species. The number of suitable photos for individual identification of naturally marked felids was greater at baited stations than at unbaited stations, but this did not result in practical advantages for individual identification. We recommend that the use of carnivore bait should be carefully considered at the planning stage of camera‐trap studies as it can negatively affect recording of prey species.
Replacement of glass in the Nakhla meteorite by berthierine: Implications for understanding the origins of aluminum‐rich phyllosilicates on Mars
Authors: Lee Martin R. , Chatzitheodoridis Elias 
Published: July 2016

AbstractA scanning and transmission electron microscope study of aluminosilicate glasses within melt inclusions from the Martian meteorite Nakhla shows that they have been replaced by berthierine, an aluminum‐iron serpentine mineral. This alteration reaction was mediated by liquid water that gained access to the glasses along fractures within enclosing augite and olivine grains. Water/rock ratios were low, and the aqueous solutions were circumneutral and reducing. They introduced magnesium and iron that were sourced from the dissolution of olivine, and exported alkalis. Berthierine was identified using X‐ray microanalysis and electron diffraction. It is restricted in its occurrence to parts of the melt inclusions that were formerly glass, thus showing that under the ambient physico‐chemical conditions, the mobility of aluminum and silicon were low. This discovery of serpentine adds to the suite of postmagmatic hydrous silicates in Nakhla that include saponite and opal‐A. Such a variety of secondary silicates indicates that during aqueous alteration compositionally distinct microenvironments developed on sub‐millimeter length scales. The scarcity of berthierine in Nakhla is consistent with results from orbital remote sensing of the Martian crust showing very low abundances of aluminum‐rich phyllosilicates.
Pulmonary interstitial emphysema due to respiratory syncytial virus infection
Authors: Aiyoshi Tsubasa , Masumoto Kouji , Shinkai Toko , Tanaka Yasunari , Fujii Shunsuke , Sasaki Takato , Chiba Fumiko , Sakamoto Naoya , Gotoh Chikashi , Urita Yasuhisa , Nakao Makoto , Takayasu Hajime , Tanaka Hideaki , Imai Hironori 
Published: July 2016

AbstractPulmonary interstitial emphysema (PIE) primarily affects premature infants on positive pressure ventilation. PIE is rarely reported in infants and children in the absence of mechanical ventilation and/or associated respiratory infection. We report a case of PIE in a 22‐month‐old girl who had severe respiratory distress due to respiratory syncytial virus infection. Chest computed tomography showed cystic lung lesions mimicking congenital cystic adenomatoid malformation. The cystic lesions spontaneously resolved after conservative treatment. Based on the clinical course and the chronological changes on imaging, the cystic lung lesions were diagnosed as localized persistent PIE.
Molecular, genetic and evolutionary analysis of a paracentric inversion in Arabidopsis thaliana
Authors: Fransz Paul , Linc Gabriella , Lee Cheng‐Ruei , Aflitos Saulo Alves , Lasky Jesse R. , Toomajian Christopher , Ali Hoda , Peters Janny , van Dam Peter , Ji Xianwen , Kuzak Mateusz , Gerats Tom , Schubert Ingo , Schneeberger Korbinian , Colot Vincent , Martienssen Rob , Koornneef Maarten , Nordborg Magnus , Juenger Thomas E. , de Jong Hans , Schranz Michael E. 
Published: July 2016

SummaryChromosomal inversions can provide windows onto the cytogenetic, molecular, evolutionary and demographic histories of a species. Here we investigate a paracentric 1.17‐Mb inversion on chromosome 4 of Arabidopsis thaliana with nucleotide precision of its borders. The inversion is created by Vandal transposon activity, splitting an F‐box and relocating a pericentric heterochromatin segment in juxtaposition with euchromatin without affecting the epigenetic landscape. Examination of the RegMap panel and the 1001 Arabidopsis genomes revealed more than 170 inversion accessions in Europe and North America. The SNP patterns revealed historical recombinations from which we infer diverse haplotype patterns, ancient introgression events and phylogenetic relationships. We find a robust association between the inversion and fecundity under drought. We also find linkage disequilibrium between the inverted region and the early flowering Col‐FRIGIDA allele. Finally, SNP analysis elucidates the origin of the inversion to South‐Eastern Europe approximately 5000 years ago and the FRI‐Col allele to North‐West Europe, and reveals the spreading of a single haplotype to North America during the 17th to 19th century. The ‘American haplotype’ was identified from several European localities, potentially due to return migration.
A practical study of white‐clawed crayfish (Austropotamobius pallipes) mitigation during in‐channel construction works, North‐East England
Authors: Latham Dorian , Bubb Damian , Hardy Maria , Guarnaccio Jon 
Published: July 2016

AbstractThe article provides a unique opportunity to study a large white‐clawed crayfish mitigation exercise on the River Wansbeck, Northumberland in North East England. The translation was necessary to allow the construction of a dam on the river to reduce flood risk to the town of Morpeth. The study provides an assessment of the methodology used for the capture and translocation activities, and provides an insight into the speed of colonisation and population establishment in a 300‐m diversion channel and the structure of translocated populations. Over 3500 + white‐clawed crayfish were caught when the natural channel was dewatered, with 5400 animals removed the following year when the diversion channel was closed. Average density based on the number of white‐clawed crayfish caught was estimated as in excess of 5 per 1 m2. The study demonstrates the importance of the River Wansbeck for white‐clawed crayfish.
Homogeneity of cohomology classes associated with Koszul matrix factorizations
Authors: Polishchuk Alexander 
Published: July 2016

In this work we prove the so-called dimension property for the cohomological field theory associated with a homogeneous polynomial $W$ with an isolated singularity, in the algebraic framework of [A. Polishchuk and A. Vaintrob, Matrix factorizations and cohomological field theories, J. Reine Angew. Math. 714 (2016), 1–122]. This amounts to showing that some cohomology classes on the Deligne–Mumford moduli spaces of stable curves, constructed using Fourier–Mukai-type functors associated with matrix factorizations, live in prescribed dimension. The proof is based on a homogeneity result established in [A. Polishchuk and A. Vaintrob, Algebraic construction of Witten’s top Chern class, in Advances in algebraic geometry motivated by physics (Lowell, MA, 2000) (American Mathematical Society, Providence, RI, 2001), 229–249] for certain characteristic classes of Koszul matrix factorizations of $0$. To reduce to this result, we use the theory of Fourier–Mukai-type functors involving matrix factorizations and the natural rational lattices in the relevant Hochschild homology spaces, as well as a version of Hodge–Riemann bilinear relations for Hochschild homology of matrix factorizations. Our approach also gives a proof of the dimension property for the cohomological field theories associated with some quasihomogeneous polynomials with an isolated singularity.
Cross-sectional study of Pfizer-sponsored clinical trials: assessment of time to publication and publication history
Authors: Mooney LaVerne A , Fay Lorna 
Published: July 2016

ObjectiveTo estimate the proportion of Pfizer-sponsored clinical trials that completed in 2010 and are published as manuscripts in the peer-reviewed literature, and to assess the manuscript development history.DesignRetrospective, cross-sectional analysis.SettingClinical trials registered in ClinicalTrials.gov that completed in 2010 for approved, Pfizer prescription products in patients or vaccines in healthy participants.Main outcome measuresThe proportion of studies for which the primary outcome(s) was published and the median time from study completion to publication. The manuscript development history included the number of times a manuscript was submitted before it was accepted for publication.ResultsAmong registered clinical trials for which Pfizer was the sponsor that completed in 2010, 76 met all inclusion criteria. The primary outcome(s) for 65 (85%) studies was published in 71 manuscripts; the median time to publication was 31 months (range 3–63 months). Of the remaining 11 studies, 2 had been submitted to at least one journal, 2 had not yet been submitted and 7 had no plans to publish because the study had terminated early due to recruitment challenges. Manuscripts accepted at the first choice journal were published at median time of 28 months (range 8–63, n=31), those accepted at second choice journal were published at 32 months (3–45, n=19), and for those accepted at third choice journal, it was 40 months (range 24–53, n=13).ConclusionsThe publication rate and median time to publication from study completion for Pfizer-sponsored studies were comparable to those previously reported for combined analyses of industry and non-industry sectors. Opportunities exist for sponsors, authors and journals to explore ideas that would facilitate more timely publication for clinical trial results. However, to be effective, such changes may need to revisit the entire publication process.
Soil fertility and fertilization practices affect accumulation and leaching risk of reactive N in greenhouse vegetable soils
Authors: Zhao Yajie , Lu Caiyan , Shi Yi , Huang Bin , Chen Xin 
Published: July 2016

Elevated soil fertility levels induced by continuous chemical fertilizer and (or) manure application may affect N loss potential and redistribution within soil-crop system. A 49-d packed soil column experiment with a factorial design of three soil fertility levels and four fertilization treatments was conducted to evaluate the effects of soil fertility and fertilization treatments on the accumulation and leaching risk of reactive N. The results showed that the 49-d cumulative leaching loss of total reactive N ranged from 176.3 to 499.0 kg N ha−1. The cumulative leaching losses of total dissolved nitrogen (TDN) and dissolved organic nitrogen (DON) in NPK treatment were significantly higher than those in other three treatments in fertility level I and II soils. The cumulative leaching loss of NO3−-N was significantly greater in NPK + M or NPK treatment than in CK treatment in fertility level I or III soils, and it was remarkably greater in M treatment than in other three treatments in fertility level II soil. In total, 64.0% of TDN in soil leachate existed in the form of DON, and 35.1% was nitrate-N among different soil fertility and fertilization treatments. These results indicated that DON was an important component of N leaching loss and could not be neglected in sustainable development of vegetable greenhouse soil.
Longitudinal Asymmetric Trends of Tropical Cold-Point Tropopause Temperature and Their Link to Strengthened Walker Circulation
Authors: Hu Dingzhu , Tian Wenshou , Guan Zhaoyong , Guo Yipeng , Dhomse Sandip 
Published: July 2016

Abstract
The zonal structure of trends in the tropical tropopause layer during 1979–2014 is investigated by using reanalysis datasets and chemistry–climate model simulations. The analysis herein reveals that the tropical cold-point tropopause temperature (CPTT) trends during 1979–2014 are zonally asymmetric; that is, over the tropical central and eastern Pacific (CEP; 20°S–20°N, 160°E–100°W), the CPTT shows an increasing trend of 0.22 K decade−1, whereas over the rest of the tropical regions (non-CEP regions) the CPTT shows a decreasing trend of −0.08 K decade−1. Model simulations suggest that this zonal asymmetry in the tropical CPTT trends can be partly attributed to Walker circulation (WC) changes induced by zonally asymmetric changes of the sea surface temperatures (SSTs). The increasing (decreasing) SSTs over the western Pacific (CEP) result in a larger zonal gradient in sea level pressure over the tropical Pacific and intensified surface easterlies. The increased pressure gradient leads to enhanced convection over the Indo-Pacific warm pool and weakened convection over the CEP, facilitating a stronger WC. The downward branch of the intensified WC induces a dynamical warming over the CEP and the upward branch of the intensified WC induces a dynamical cooling over the non-CEP regions below 150 hPa. The significant warming in the upper troposphere and lower stratosphere (UTLS) caused by the WC descending and wave activity changes in the UTLS over the CEP shifts the cold-point tropopause height to a higher level, while the radiative effects of greenhouse gases, ozone, and water vapor changes in the UTLS make less important contributions to the trend of the tropical CPTT than SST changes.
In-silico discovery of cancer-specific peptide-HLA complexes for targeted therapy
Authors: Dhanik Ankur , R. Kirshner Jessica , MacDonald Douglas , Thurston Gavin , C. Lin Hsin , J. Murphy Andrew , Zhang Wen 
Published: July 2016

Abstract
Background
Major Histocompatibility Complex (MHC) or Human Leukocyte Antigen (HLA) Class I molecules bind to peptide fragments of proteins degraded inside the cell and display them on the cell surface. We are interested in peptide-HLA complexes involving peptides that are derived from proteins specifically expressed in cancer cells. Such complexes have been shown to provide an effective means of precisely targeting cancer cells by engineered T-cells and antibodies, which would be an improvement over current chemotherapeutic agents that indiscriminately kill proliferating cells. An important concern with the targeting of peptide-HLA complexes is off-target toxicity that could occur due to the presence of complexes similar to the target complex in cells from essential, normal tissues.

Results
We developed a novel computational strategy for identifying potential peptide-HLA cancer targets and evaluating the likelihood of off-target toxicity associated with these targets. Our strategy combines sequence-based and structure-based approaches in a unique way to predict potential off-targets. The focus of our work is on the complexes involving the most frequent HLA class I allele HLA-A*02:01. Using our strategy, we predicted the off-target toxicity observed in past clinical trials. We employed it to perform a first-ever comprehensive exploration of the human peptidome to identify cancer-specific targets utilizing gene expression data from TCGA (The Cancer Genome Atlas) and GTEx (Gene Tissue Expression), and structural data from PDB (Protein Data Bank). We have thus identified a list of 627 peptide-HLA complexes across various TCGA cancer types.

Conclusion
Peptide-HLA complexes identified using our novel strategy could enable discovery of cancer-specific targets for engineered T-cells or antibody based therapy with minimal off-target toxicity.
Efficacy of Tumor Vaccines and Cellular Immunotherapies in Non–Small-Cell Lung Cancer: A Systematic Review and Meta-Analysis
Authors: Dammeijer Floris , Lievense Lysanne A. , Veerman G.D. Marijn , Hoogsteden Henk C. , Hegmans Joost P. , Arends Lidia R. , Aerts Joachim G. 
Published: July 2016

Purpose Programmed cell death protein-1- checkpoint blockers have recently been approved as second-line treatment for advanced non–small-cell lung cancer (NSCLC). Unfortunately, only a subgroup of patients responds and shows long-term survival to these therapies. Tumor vaccines and cellular immunotherapies could synergize with checkpoint blockade, but which of these treatments is most efficacious is unknown. In this meta-analysis, we assessed the efficacy of tumor vaccination and cellular immunotherapy in NSCLC. Methods We searched for randomized controlled trials (RCTs) investigating cellular immunotherapy or vaccines in NSCLC. We used random effects models to analyze overall survival (OS) and progression-free survival (PFS), expressed as hazard ratios (HRs), and differences in time (months). The effect of immunotherapy type, disease stage, tumor histology, and concurrent chemotherapy was assessed using subgroup analysis and meta-regression. All procedures were performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Results We identified 18 RCTs that matched our selection criteria; these included a total of 6,756 patients. Immunotherapy extended NSCLC survival and PFS, expressed as HR (OS: HR, 0.81, 95% CI, 0.70 to 0.94, P = .01; PFS: HR, 0.83, 95% CI, 0.72 to 0.95, P = .006) and month difference (OS: difference, 5.43 months, 95% CI, 3.20 to 7.65, P < .005; PFS: difference, 3.24 months, 95% CI, 1.61 to 4.88, P < .005). Cellular therapies outperformed tumor vaccines (OS as HR: P = .005, month difference: P < .001; PFS as HR: P = .001, month difference: P = .004). There was a benefit of immunotherapy in low-stage compared with high-stage NSCLC and with concurrent administration of chemotherapy only in one of four outcome measures evaluated (PFS in months: P = .01 and PFS as HR: P = .031, respectively). There was no significant effect of tumor histology on survival or PFS. Conclusion Tumor vaccines and cellular immunotherapies enhanced OS and PFS in NSCLC. Cellular immunotherapy was found to be more effective than tumor vaccination. These findings have implications for future studies investigating combination immunotherapy in NSCLC.