bluuebunny/crossref_metadata_2025_split huggingface dataset:
The efficacy of commercial tooth storage media for maintaining the viability of human periodontal ligament fibroblasts
Authors: Lee W. , Stover S. , Rasoulianboroujeni M. , Sherman K. , Fahimipour F. , Dashtimoghadam E. , Zito C. , Jazayeri H. E. , Tayebi L. 
Published: June 2017

AbstractAimTo evaluate Save‐A‐Tooth (SAT), EMT Toothsaver (EMT) and Hank's Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs).MethodologyPrimary HPDLFs were seeded into 96‐well cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 h at room temperature (22 °C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (ATP) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24 h storage in each medium. The data were analysed statistically by two‐way anova and post hoc Least Significant Difference (LSD) test (P < 0.05). The morphology of the cells after 12 h storage was also investigated through live/dead viability/cytotoxicity kit together with fluorescence microscopy.ResultsThere was no significant difference in cell viability amongst HBSS, SAT and EMT groups up to 6 h. SAT was effective in maintaining cell viability only up to 12 h and then became detrimental to HPDLF; after 24 h, the effectiveness of SAT in maintaining cell viability was similar to that of water (P > 0.05). Amongst all the media, only EMT could maintain the proliferative capacity of HPDLFs significantly higher than the negative control, that is water (P < 0.05) after 24 h storage.ConclusionEMT maintained the proliferative capacity of HPDLFs after 24 h storage.
Functional analysis of female‐biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze)
Authors: Sun L. , Wang Q. , Yang S. , Wang Q. , Zhang Z. , Khashaveh A. , Zhang Y.‐J. , Guo Y.‐Y. 
Published: June 2017

AbstractThe polyphagous mirid bug Adelphocoris lineolatus relies heavily on olfactory cues to track suitable host plants. Thus, a better understanding of the molecular basis of its olfactory detection could contribute to the development of effective pest management strategies. In the present study, we report the expression profile of the odorant binding protein gene A. lineolatus odorant binding protein 6 (AlinOBP6). Quantitative real‐time PCR experiments suggest that AlinOBP6 is female adult antennae‐biased. Cellular immunolocalization analyses show that AlinOBP6 is highly expressed in the lymph of both multiporous sensilla basiconica and uniporous sensilla chaetica. A ligand binding analysis showed that recombinant AlinOBP6 not only bound tightly to host plant volatile compounds but also to nonvolatile compounds. Homology modelling and molecular docking analyses confirmed these unusual ligand binding profiles and revealed that the amino acid residues involved in the recognition of volatile and nonvolatile compounds are distinct. The results of our study are the first to suggest that an antenna‐ and female‐biased OBP in an hemipteran insect is expressed in both olfactory and gustatory sensilla as a mechanism to respond to volatile and nonvolatile host compounds. These findings warrant further research into the molecular mechanisms of chemosensation for mirid bugs in responsive to host plant location.
Development of a colloidal gold immunochromatographic assay (GICA) for the rapid detection of Spiroplasma eriocheiris in commercially exploited crustaceans from China
Authors: Liu H , Xiu Y , Xu Y , Tang M , Li S , Gu W , Meng Q , Wang W 
Published: June 2017

AbstractSpiroplasma eriocheiris is an emerging pathogen in freshwater crustaceans. In recent years, Eriocheir sinensis, Procambarus clarkii, Litopenaeus vannamei, Macrobrachium rosenbergii and Macrobrachium nipponense had been infected by this pathogen in China. An immunochromatographic strip test using gold nanoparticles was developed for rapidly detecting this pathogen. The strip test based on the principle of sandwich immunoassay by the specific combination between the pathogen and polyclonal antibody on a nitrocellulose membrane. Positive samples were displayed as red lines at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red line only at the control zone. The limit of detection was proved to be 106 Color Change Unit/ml. The test strip could be visually detected within 15 min and do not have cross‐reaction with other aquatic bacteria. This test strip allows on‐site rapid detection of S. eriocheiris in crustacean without the requirement of specialized equipment and professional personnel. The one‐step test strips developed in our study had high sensitivity, specificity, reproducibility and stability. In conclusion, this method was proved to be convenient, feasible, rapid and effective for detecting S. eriocheiris.
Grasping damage analysis of apple by end‐effector in harvesting robot
Authors: Ji Wei , Qian Zhijie , Xu Bo , Tang Wei , Li Junle , Zhao Dean 
Published: June 2017

AbstractAccording to the grasping damage of apple during the process of robot picking apple, the variation of interior tensions inside the apple skin in the grasping process of apple with different type finger of robot end‐effector is researched. The finite element model for apple is established by ANSYS. Some simulations for the grasping process of apple with plane and arc‐shaped finger are carried out. The Von Mises stress nephograms of apple different tissue under different load force by different type fingers are obtained. The experimental results show that the apple cortex is more easily to get damaged due to its small failure stress. And the deformation and stress of apple caused by arc‐shaped finger are smaller than by plane finger. At last, the actual experiment for apple grasping damage of end‐effector with arc‐shaped finger validates the reliability of simulated results. The research results demonstrated that the finite element method can make accurate evaluation for apple damage.Practical applicationsA major problem associated with robot harvesting is the mechanical damage of apple caused by end‐effector of robot. When the apple is grasped by the end‐effector, the mechanical damage is often occurred underneath apple skin thus, which is difficult to find by the naked eye immediately. The results in our paper can accurate evaluation for apple damage and provide a foundational basis to develop an injury‐reduce device of apple harvesting robot.
Rapid determination of oil quantity in intact rapeseeds using near‐infrared spectroscopy
Authors: Wan Linsheng , Sun Hongqin , Ni Zhengbin , Yan Guohong 
Published: June 2017

AbstractThe present study aims to predict the oil quantity in % using Near‐infrared spectroscopy (NIRS) directly from spectral data acquired on rapeseeds (Brassica napus). In total 637 samples were used to construct and test the mathematic model. The correlation coefficient (R2) between the NIRS predicated results and the chemical extraction method was .98, .97, and .96 for the calibration, cross validation, and external validation, respectively. For external validation, the average relative error and the mean absolute error was 0.78 and 2.02%, respectively, compatible with those of chemical extraction method. Further determination suggested that 1.5 g was the minimal quantity of sample loading to obtain stable results and the NIRS method showed a similar repeatability to the chemical extraction method. Taken together, NIRS method enables the rapid detection of oil quantity of intact B. napus seeds.Practical applicationsThe present study constructed a mathematic model for the measurement of oil content of rapeseed using the NIRS technology. Compared with the traditional method for oil content determination using the chemical extraction, the NIRS method is cheaper, safer, more rapid, and convenient. It shows similar repeatability to the chemical extraction method and needs not to destroy the samples. Thus, the method raised in the present study could be used to detect the oil content of intact rapeseeds in food and breeding industries.
A Typology of Interprofessional Teamwork in Acute Geriatric Care: A Study in 55 units in Belgium
Authors: Piers Ruth D. , Versluys Karen J. J. , Devoghel Johan , Lambrecht Sophie , Vyt André , Van Den Noortgate Nele J. 
Published: June 2017

ObjectivesTo explore the quality of interprofessional teamwork in acute geriatric care and to build a model of team types.DesignCross‐sectional multicenter study.SettingAcute geriatric units in Belgium.ParticipantsTeam members of different professional backgrounds.MeasurementsPerceptions of interprofessional teamwork among team members of 55 acute geriatric units in Belgium were measured using a survey covering collaborative practice and experience, managerial coaching and open team culture, shared reflection and decision‐making, patient files facilitating teamwork, members’ belief in the power of teamwork, and members’ comfort in reporting incidents. Cluster analysis was used to determine types of interprofessional teamwork. Professions and clusters were compared using analysis of variance.ResultsThe overall response rate was 60%. Of the 890 respondents, 71% were nursing professionals, 20% other allied health professionals, 5% physicians, and 4% logistic and administrative staff. More than 70% of respondents scored highly on interprofessional teamwork competencies, consultation, experiences, meetings, management, and results. Fewer than 55% scored highly on items about shared reflection and decision‐making, reporting incidents from a colleague, and patient files facilitating interprofessional teamwork. Nurses in this study rated shared reflection and decision‐making lower than physicians on the same acute geriatric units (P < .001). Using the mean score on each of the six areas, four clusters that differed significantly in all areas were identified using hierarchical cluster analysis and scree plot analysis (P < .001).ConclusionInterprofessional teamwork in acute geriatric units is satisfactory, but shared reflection and decision‐making needs improvement. Four types of interprofessional teamwork are identified and can be used to benchmark the teamwork of individual teams.
The malleable brain: plasticity of neural circuits and behavior – a review from students to students
Authors: Schaefer Natascha , Rotermund Carola , Blumrich Eva‐Maria , Lourenco Mychael V. , Joshi Pooja , Hegemann Regina U. , Jamwal Sumit , Ali Nilufar , García Romero Ezra Michelet , Sharma Sorabh , Ghosh Shampa , Sinha Jitendra K. , Loke Hannah , Jain Vishal , Lepeta Katarzyna , Salamian Ahmad , Sharma Mahima , Golpich Mojtaba , Nawrotek Katarzyna , Paidi Ramesh K. , Shahidzadeh Sheila M. , Piermartiri Tetsade , Amini Elham , Pastor Veronica , Wilson Yvette , Adeniyi Philip A. , Datusalia Ashok K. , Vafadari Benham , Saini Vedangana , Suárez‐Pozos Edna , Kushwah Neetu , Fontanet Paula , Turner Anthony J. 
Published: June 2017

AbstractOne of the most intriguing features of the brain is its ability to be malleable, allowing it to adapt continually to changes in the environment. Specific neuronal activity patterns drive long‐lasting increases or decreases in the strength of synaptic connections, referred to as long‐term potentiation and long‐term depression, respectively. Such phenomena have been described in a variety of model organisms, which are used to study molecular, structural, and functional aspects of synaptic plasticity. This review originated from the first International Society for Neurochemistry (ISN) and Journal of Neurochemistry (JNC) Flagship School held in Alpbach, Austria (Sep 2016), and will use its curriculum and discussions as a framework to review some of the current knowledge in the field of synaptic plasticity. First, we describe the role of plasticity during development and the persistent changes of neural circuitry occurring when sensory input is altered during critical developmental stages. We then outline the signaling cascades resulting in the synthesis of new plasticity‐related proteins, which ultimately enable sustained changes in synaptic strength. Going beyond the traditional understanding of synaptic plasticity conceptualized by long‐term potentiation and long‐term depression, we discuss system‐wide modifications and recently unveiled homeostatic mechanisms, such as synaptic scaling. Finally, we describe the neural circuits and synaptic plasticity mechanisms driving associative memory and motor learning. Evidence summarized in this review provides a current view of synaptic plasticity in its various forms, offers new insights into the underlying mechanisms and behavioral relevance, and provides directions for future research in the field of synaptic plasticity.
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Read the Editorial Highlight for this article on page 788.Cover Image for this issue: doi: 10.1111/jnc.13815.
Effects of Messages Delivered by Mobile Phone on Increasing Compliance With Shoulder Exercises Among Patients With a Frozen Shoulder
Authors: Chen Hui‐Chun , Chuang Tai‐Yuan , Lin Pi‐Chu , Lin Yen‐Kuang , Chuang Yeu‐Hui 
Published: June 2017

AbstractPurposeThe aim of this study was to examine the effects of reminders, encouragement, and educational messages delivered by mobile phone on shoulder exercise compliance and improvements in shoulder function among patients with a frozen shoulder.Design and MethodsA randomized controlled trial design was used. A convenience sample of patients with a frozen shoulder in an orthopedic outpatient clinic was recruited. All participants were instructed on how to do shoulder exercises and were provided with a printed pamphlet about shoulder exercises. Then, the intervention group received reminders, encouragement, and educational messages by mobile phone daily for the next 2 weeks, while the comparison group did not.FindingsThe intervention group had higher compliance with shoulder exercises than did the comparison group (t = 2.263, p = .03) and had significant improvements in shoulder forward flexion (F = 12.067, p = .001), external rotation (F = 13.61, p = .001), and internal rotation (F = 5.903, p = .018) compared to those in the comparison group after the 2‐week intervention.ConclusionsThe text messages significantly increased patient compliance with shoulder exercises and thus improved patients’ shoulder range of motion.Clinical RelevanceHospital or clinics can send appropriate messages to patients via text message platforms in order to remind and encourage them to do shoulder exercises.
Oxidative stress and psychiatric morbidity in patients with facial acne
Authors: Awad Sara M. , Morsy Hanan , Sayed Ayat A. , Mohamed Nahed A. , Ezzat Ghada M. , Noaman Mostafa M. 
Published: June 2017

SummaryBackgroundAcne vulgaris is a common cosmetic problem that is frequently associated with psychosocial disturbances as well as increased oxidative stress. However, oxidative stress and psychological aspects have been studied separately in acne.ObjectiveTo evaluate the relationships between oxidative stress, anxiety, depression, and quality of life in acne patients.MethodsSixty patients with facial acne and 40 age‐ and sex‐matched healthy individuals were included in the study. Anxiety and depression were assessed using the Hospital Anxiety and Depression Scale (HADS), and quality of life (QoL) was measured by the Cardiff Acne Disability Index. Disease severity was assessed using the Combined Acne Severity Classification. The serum levels of zinc and malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured in patients and healthy subjects.ResultsThe mean HADS scores for anxiety and depression were higher in patients than controls (P<.001 for both). Acne patients showed higher serum MDA and lower TAC and serum zinc levels compared with control subjects (P=.019, P<.001, and P=.028, respectively). Anxiety and depression scores did not correlate with oxidative stress parameters. Patients with moderate/severe acne had worse anxiety scores than mild acne (P=.048), and higher anxiety scores were associated with poorer quality of life (r=.436, P=.001).ConclusionOur results indicate that the high levels of anxiety and depression in patients with facial acne were not related to oxidative stress. Anxiety was more common than depression and was directly related to QoL impairment.
Nursing assessment as an effective tool for the identification of delirium risk in older in‐patients: A case–control study
Authors: Solà‐Miravete Elena , López Carlos , Martínez‐Segura Estrella , Adell‐Lleixà Mireia , Juvé‐Udina Maria Eulàlia , Lleixà‐Fortuño Mar 
Published: June 2017

Aims and objectivesTo evaluate the usefulness of comprehensive nursing assessment as a strategy for determining the risk of delirium in older in‐patients from a model of care needs based on variables easily measured by nurses.BackgroundThere are many scales of assessment and prediction of risk of delirium, but they are little known and infrequently used by professionals. Recognition of delirium by doctors and nurses continues to be limited.Design and methodsA case–control study. A specific form of data collection was designed to include the risk factors for delirium commonly identified in the literature and the care needs evaluated from the comprehensive nursing assessment based on the Virginia Henderson model of care needs. We studied 454 in‐patient units in a basic general hospital. Data were collected from a review of the records of patients’ electronic clinical history.ResultsThe areas of care that were significant in patients with delirium were dyspnoea, problems with nutrition, elimination, mobility, rest and sleep, self‐care, physical safety, communication and relationships. The specific risk factors identified as independent predictors were as follows: age, urinary incontinence, urinary catheter, alcohol abuse, previous history of dementia, being able to get out of bed/not being at rest, habitual insomnia and history of social risk.ConclusionsComprehensive nursing assessment is a valid and consistent strategy with a multifactorial model of delirium, which enables the personalised risk assessment necessary to define a plan of care with specific interventions for each patient to be made.Relevance to clinical practiceThe identification of the risk of delirium is particularly important in the context of prevention. In a model of care based on needs, nursing assessment is a useful component in the risk assessment of delirium and one that is necessary for developing an individualised care regime.
Genetic landscape and deregulated pathways in B‐cell lymphoid malignancies
Authors: Rosenquist R. , Beà S. , Du M.‐Q. , Nadel B. , Pan‐Hammarström Q. 
Published: June 2017

AbstractWith the introduction of next‐generation sequencing, the genetic landscape of the complex group of B‐cell lymphoid malignancies has rapidly been unravelled in recent years. This has provided important information about recurrent genetic events and identified key pathways deregulated in each lymphoma subtype. In parallel, there has been intense search and development of novel types of targeted therapy that ‘hit’ central mechanisms in lymphoma pathobiology, such as BTK, PI3K or BCL2 inhibitors. In this review, we will outline the current view of the genetic landscape of selected entities: follicular lymphoma, diffuse large B‐cell lymphoma, mantle cell lymphoma, chronic lymphocytic leukaemia and marginal zone lymphoma. We will detail recurrent alterations affecting important signalling pathways, that is the B‐cell receptor/NF‐κB pathway, NOTCH signalling, JAK‐STAT signalling, p53/DNA damage response, apoptosis and cell cycle regulation, as well as other perhaps unexpected cellular processes, such as immune regulation, cell migration, epigenetic regulation and RNA processing. Whilst many of these pathways/processes are commonly altered in different lymphoid tumors, albeit at varying frequencies, others are preferentially targeted in selected B‐cell malignancies. Some of these genetic lesions are either involved in disease ontogeny or linked to the evolution of each disease and/or specific clinicobiological features, and some of them have been demonstrated to have prognostic and even predictive impact. Future work is especially needed to understand the therapy‐resistant disease, particularly in patients treated with targeted therapy, and to identify novel targets and therapeutic strategies in order to realize true precision medicine in this clinically heterogeneous patient group.
The mobile dso-gene-sso element in rolling-circle plasmids of staphylococci reflects the evolutionary history of its resistance gene
Authors: Wassenaar T.M. , Cabal A. 
Published: June 2017

Abstract
The qacC and lnuA genes of Staphylococcus species were recently proposed to comprise a mobile element when residing on rolling-circle plasmids. Here we present other examples of resistance genes on staphylococcal rolling-circle plasmids, including fosB producing resistance to fosfomycin, cat resulting in resistance to chloramphenicol and cadB for resistance to the toxic heavy metal cadmium. For three of these genes (qacC, lnuA and fosB), evidence was obtained that the genes have spread between different plasmid backgrounds. The lack of mutations in qacC suggests that the spread occurred relatively recently, while the build up of mutations in lnuA and fosB suggests their mobilization occurred in the more distant past. These observations can be explained by the use of the respective antibiotics over time. However, the cat and cadB genes sequences analysed had not collected any mutations, an observation that is not completely understood but possible explanations are discussed.

Significance and Impact of the Study
We have analysed five resistance genes in Staphylococcus aureus that are positioned between the replication elements of rolling-circle plasmids. For three of these genes, evidence was obtained indicative of recent mobilization. The historical use of the antibiotics to which the genes produce resistance could be related to the number of mutations collected in these genes. However, two other resistance genes have not collected any mutations over time, and the reasons for this are discussed. The analyses presented provide insights into the spread and evolution of antibiotic resistance genes.