bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Oxidative Phosphorylation-Related Subtypes and
Construction of a Prognostic Signature in Ovarian Cancer
Authors: Lu Jiaojiao , Zhen Shuai , Li Xu 
Published: September 2024

Background:
Ovarian cancer is associated with a high mortality rate. Oxidative Phosphorylation
(OXPHOS) is an active metabolic pathway in cancer; nevertheless, its role in ovarian
cancer continues to be ambiguous. Therefore, the objective of this study was to identify the prognostic
value of OXPHOS-related genes and the immune landscape in ovarian cancer.

Methods:
We obtained public ovarian cancer-related datasets from The Cancer Genome Atlas
(TCGA) and Gene Expression Omnibus (GEO) databases and recognized OXPHOS-related genes
from the GeneCards database and literature. Cox regression analyses were conducted to identify
prognostic OXPHOS-related genes and develop a prognostic nomogram based on the OXPHOS
score and clinicopathological features of patients. Functional enrichment analyses were employed
to identify related processes.

Results:
A 12-gene signature was identified to classify the ovarian cancer patients into high- and
low-risk groups. The Immunophenoscore (IPS) was higher in the OXPHOS score-high group than
in the OXPHOS score-low group, suggesting a better response to immune checkpoint inhibitors.
Functional enrichment analyses unveiled that OXPHOS-related genes were considerably abundant
in a series of immune processes. The calibration curves of the constructed prognostic nomograms at
1, 2, and 3 years exhibited strong concordance between the anticipated and observed survival probabilities
of ovarian cancer patients.

Conclusion:
We have constructed a prognostic model containing 12 OXPHOS-related genes and
demonstrated its strong predictive value in ovarian cancer patients. OXPHOS has been found to be
closely linked to immune infiltration and the reaction to immunotherapy, which may contribute to
improving individualized treatment and prognostic evaluation in ovarian cancer.
Exploring the Insights on Exosomes and their Utility in Treating Ophthalmic Disease: Delving into the Clinical Approval and Present Trials
Authors: Dwivedi Ashish Ranjan , Murti Yogesh , Rawat Pramod , Mahajan Shriya , Kandhari Harsimrat , Joshi Gaurav , Kumar Bhupinder 
Published: September 2024

Abstract:
Ophthalmic diseases include a wide array of conditions, each requiring individualized
treatment approaches. In ophthalmic research and as therapeutics against potential pharmacological
indications, several subtypes of exosomes (EVs) have been reconnoitered, mainly for their regenerative,
neuroprotective, and anti-inflammatory characteristics. EVs are recently gaining wider
attention as promising vehicles for therapies because of their natural participation in communication
between cells and targeted delivery. These small vesicles, derived from cells, transport numerous
molecules between cells, thus contributing advantages like low immunogenicity, stability, and
the ability to target cells specifically. These inherent advantages of carrying the therapeutic cargo
and enabling intercellular signaling make them a captivating avenue for progressing ophthalmic
disease treatment options. While research is ongoing, and clinical applications are still emerging,
several EV subtypes have shown promise for possible applications in addressing several ophthalmic
diseases, such as glaucoma, age-related macular degenerative disorders, retinal degenerative
disorders, and ocular inflammatory conditions.
Research on Compatibility of Packaging Materials of High-Risk Cephalosporin Powder Injection and Establishment of Indicator Component Evaluation Method
Authors: Chen Chao , Wang Yan , Chen Yue , Zhang Aijing , Cai Zhiwei , Cheng Lei , Xu Bingyong , Bao Xinyou , Liang Jianmou 
Published: September 2024

Objective:
The aim of the study was to establish a GC-MS method for the determination
of extractable substances in the stoppers of Ceftriaxone sodium bottles for injection, to establish
an HPLC method for the determination of vulcanizing agent and antioxidant in Ceftriaxone
sodium for injection bottle stopper and to analyze the correlation between the volatile substances
of the rubber stopper and the clarity of solution

Methods:
GC-MS method was used with capillary column Restek rtx-5 (30.0 m×0.25 mm, 0.25
μm), the carrier gas was He at the flow rate of 1.8mL·min-1, the column temperature was programmed
temperature, and the scanning range was from29 to 600(m/z), and the volatile substances
were using full scan. An inertsil ODS-3column(250 mm×4.6 mm, 5 μm) was used for the
HPLC analysis. The mobile phase A consisted of water containing 0.04% trifluoroacetic, and the
mobile phase B consisted of acetonitrile containing 0.04% trifluoroacetic acid, the gradient elution
was used, and the flow rate was set as 1.0 mL·min-1.

Results:
Antioxidant BHT(264), silicone oil, and other organic compounds in the rubber stopper
permeated and migrated to the sample with acceleration time by GC-MS. A good linearity was observed
over the range of 0.5-100 μg·mL-1(r>0.999). The detection limits of the vulcanizing agent
and antioxidant were about 0.03 μg·mL-1, and the quantitation limits were about 0.1 μg·mL-1. The
average recoveries (low, medium, and high) of antioxidants (168, 264, 330, 1076, 1010) and vulcanizing
agents (carbon disulfide, sulfur) were between 90.0% and 110.0%, and the corresponding
RSD were less than 5% (n=9) by adding standard recovery experiments. The stability test showed
that the antioxidant and the vulcanizing agent solution had good stability and was stable within 24
hours.

Conclusion:
The GC-MS/HPLC method can be used to control the quality of the rubber stopper
of cefuroxime sodium for injection. The compatibility study and comprehensive evaluation system
of Ceftriaxone sodium for injection packaging materials have been initially formed.
Universally Applicable Methods for Comprehensive Risk Assessment of Elemental Impurities in Vitamin A and D Preparations
Authors: Qiao Zhang , Mengmeng Shen , Li Zhu , Chaoqiang Xiao , Shuwang He , Jie Yang 
Published: September 2024

Background:
Vitamin A and D deficiency in children is a common public health
problem. In China, almost all children are administered vitamin A and D preparations daily and
they should be consumed continuously until the age of 18. Children are sensitive to elemental impurities,
including heavy metals. However, there are no regulatory requirements or reports on the
risk assessment of elemental impurities in marketed vitamin A and D preparations.

Objective:
The aim of this study was to propose an accurate and efficient method suitable for samples
from different manufacturers to detect elemental impurities and conduct risk assessments according
to the ICH Q3D guidelines.

Methods:
We developed a universally applicable digestion method for capsules and an ICP–MS
method for quantitative analysis of 28 elemental impurities in vitamin A and D formulations. These
methods were validated according to the USP 233 guidelines. Elemental impurities in 15 batches
of vitamin A and D products from 10 manufacturers and their capsule shells were determined,
and risk assessment was conducted according to the International Council for Harmonization Q3D
guidelines.

Results:
All elemental impurities of toxicological concern met the Q3D requirements. However,
the concentrations of various elements, including those of lead (not detected to 4.1 μg/g), arsenic
(not detected to 7 μg/g), aluminum (not detected to 888 μg/g), and palladium (not detected to 36
μg/g), varied markedly. Moreover, the lead content in one batch from one manufacturer exceeded
the control threshold.

Conclusion:
In this study, we successfully developed an effective digestion method for processing
capsules containing samples from different sources and a sensitive ICP–MS method for quantitative determination of 28 elemental impurities in vitamin A and D preparations. ICP–MS should be
implemented for the evaluation and control of elemental impurities in vitamin A and D preparations. This will ensure safe long-term vitamin A and D supplementation in children
Simultaneous Estimation of Pregabalin and Duloxetine, Used to Treat Nerve Pain, by Stability Indicating RP-HPLC Method Using the QBD Approach
Authors: Gosavi Vaishnavi A. , Deshpande Mahesh M. , Ghangale Gauri D. , Chavan Machindra J. 
Published: September 2024

Objective:
A novel stability indicating RP-HPLC method was developed and validated
for the simultaneous estimation of Pregabalin and Duloxetine by using the QBD approach.

Methods:
To determine the optimal parameters for the duloxetine and pregabalin study, we used
Design Expert version 13.0 in this work. The mobile phase consisted of 0.8 ml/min of methanol:
water (80:20, v/v) with o-phosphoric acid-adjusted pH 3.A 250 mm × 4.6 mm ID, 5 μm particle
size Cosmosil C18 column was used. The UV-3000-M detector was used to detect at 218 nm.

Results:
Duloxetine's retention time was 7.153 ± 0.1 min, whereas Pregabalin's was 4.481±0.1 min.
The ICH parameters like linearity, accuracy, robustness, ruggedness, LOD and LOQ, and system
suitability were determined. Likewise, the tailing factors were found to be 1.27 and 1.26, while the
theoretical plates of duloxetine and pregabalin were found to be 8313 and 8549, respectively. The
recovery study shows the results (99.37–99.84% for pregabalin and 99.03–99.74% for duloxetine),
and the precision was obtained with a %RSD of less than 2%. The result of the assay was 99.92 %
for pregabalin and 99.39% for duloxetine. The developed method was used for the forced degradation
study. The Box-Behnken design experiment using Design Expert 13.0 was adopted for the
QBD approach.

Conclusion:
All the values obtained for different parameters were within the acceptable range of
ICH. The developed method can be routinely used for the simultaneous estimation of Pregabalin
and Duloxetine by using the QBD approach.
Assessing the Evidence for Nonobstetric Risk Factors for Deformational Plagiocephaly: Systematic Review and Meta-Analysis
Authors: Hillyar Christopher Robert Timothy , Bishop Natalie , Nibber Anjan , Bell-Davies Frances Jean , Ong Juling 
Published: September 2024

Background
Plagiocephaly is defined as an asymmetrical distortion of the skull, resulting in an oblique trapezoid or parallelogram head shape. Deformational plagiocephaly (DP) is caused by forces acting on one side of the back of the head, distorting normal skull symmetry.

Objective
The aims of this systematic review and meta-analysis were to critically assess the evidence for nonobstetric risk factors for DP and to make evidence-based recommendations for reducing the prevalence of DP.

Methods
The selection criterion was studies reporting risk factors for DP. Case reviews, case series, expert opinions, and systematic reviews were excluded. PubMed and Web of Science were searched from August 21, 2010, to August 21, 2022. Publication bias was assessed using funnel plots. Meta-analyses were presented using forest plots.

Results
A total of 19 studies (cohort studies: n=13, 68%; case-control studies: n=5, 26%; and cross-sectional studies: n=1, 5%) with a total of 14,808 participants were included. Of the 43 investigated potential nonobstetric factors, 16 (37%) were associated with DP. Of these 16 factors, 12 (75%) had odds ratios (ORs) with 95% CIs not crossing 1: insufficient vitamin D intake (OR 7.15, 95% CI 3.77-13.54), head position preference (OR 4.75, 95% CI 3.36-6.73), bottle-only feeding (OR 4.65, 95% CI 2.70-8.00), reduced tummy time (OR 3.51, 95% CI 1.71-7.21), sleeping position (OR 3.12, 95% CI 2.21-4.39), fewer motor milestones reached by the age of 6 months (OR 2.56, 95% CI 1.66-3.96), obesity (OR 2.45, 95% CI 1.02-5.90), maternal education level (OR 1.66, 95% CI 1.17-2.37), male sex (OR 1.51, 95% CI 1.07-2.12), formula feeding (OR 1.51, 95% CI 1.00-2.27), head circumference (OR 1.22, 95% CI 1.06-1.40), and mechanical ventilation (OR 1.10, 95% CI 1.00-1.14). No evidence of publication bias was detected.

Conclusions
This study provides a comprehensive assessment of the nonobstetric factors associated with DP and presents 11 evidence-based recommendations for reducing its prevalence. The primary limitation is that only publication bias was assessed.

Trial Registration
PROSPERO CRD42020204979; https://www.crd.york.ac.uk/prospero/display_record.php? ID=CRD42020204979
Education Program for Enhancing Health Care Students’ Attitudes Toward People Living With Dementia: Protocol for a Single-Arm Pre-Post Study
Authors: Sari Dianis Wulan , Kugai Haruna , Igarashi Ayumi , Takaoka Manami , Matsumoto Hiroshige , Suzuki Haruno , Wu Jinyan , Fitryasari Rizki , Nasifah Ike Ayunda , Has Eka Mishbahatul M , Yamamoto-Mitani Noriko 
Published: September 2024

Background
Health care students are instrumental in shaping the future of dementia care. Cultivating a positive attitude and understanding toward people living with dementia is crucial for diminishing the stigma associated with the condition, providing effective and person-centered care, and enhancing the quality of life for people living with dementia. Educational programs about dementia are increasingly recognizing the potential of gaming tools.

Objective
This study aimed to evaluate the effectiveness of gaming-based dementia educational programs in improving attitudes toward people living with dementia among health care students.

Methods
This single-arm pre-post study will be conducted among health care students in Indonesian universities. This educational program based on gaming tools will consist of a lecture on dementia, the use of N-impro (gaming tool), and the enactment of short dramas depicting desirable and undesirable communication with people living with dementia behaviors. We will assess attitudes toward people living with dementia, intention to help people living with dementia, knowledge of dementia, and the stigma associated with people living with dementia. The gaming-based dementia education program will be integrated into the curriculum of the health care students. The program will be implemented once with a duration of 90 minutes.

Results
Data collection will occur from August 2023 to March 2024. Analysis of the data will be finalized by May 2024, and the outcome will be determined by July 2024. The impact of the gaming-based dementia educational program on improving attitudes toward people living with dementia will be reported. The study findings will be published in a peer-reviewed journal.

Conclusions
The gaming education program demonstrates significant potential in enhancing attitudes toward people living with dementia across various countries, introducing an innovative method for the community-based support of people living with dementia.

Trial Registration
ClinicalTrials.gov NCT06122623; https://clinicaltrials.gov/study/NCT06122623

International Registered Report Identifier (IRRID)
DERR1-10.2196/62654
New Record of A Freshwater Prawn <i>Macrobrachium sundaicum</i> in Selat Panjang Island, Riau Province, Indonesia
Authors: Purnamasari Lora , Perwitasari-Farajallah Dyah , Wowor Daisy , Farajallah Achmad , Annawaty Annawaty 
Published: September 2024

A freshwater prawn M. sundaicum, is an obligate species to acidic peat swamp. Up to the present, M. Sundaicum has only been reported in the West Kalimantan, the Riau Archipelago, and Jambi Provinces in Indonesia. The aim of this research is to determine the distribution and habitat preferences of peat swamp prawn in Selat Panjang Island, Riau Province, Indonesia. The samples were collected in seven peat swamp rivers by hand net. The study yielded one hundred specimens. The acidic peat swamp is a perfect habitat for M. sundaicum. This study provided the basic information about peat swamp prawn in Selat Panjang Island, especially their distribution and habitat preferences.
New prognostic model for liver transplantation outcomes in hepatocellular carcinoma
Authors: Voskanyan S. E. , Rudakov V. S. , Sushkov A. I. , Popov M. V. , Bashkov A. N. , Gubarev K. K. , Artemyev A. I. , Kolyshev I. Yu. , Muktazhan M. , Pashkov A. N. , Naydenov E. V. , Svetlakova D. S. 
Published: September 2024

Background. Liver transplantation remains a priority treatment option for hepatocellular carcinoma in the presence of liver cirrhosis; yet precise outcome prediction post-operation continues to be a complex challenge. Existing prognostic model often overlook patient age and donor type. Enhanced models that incorporate these parameters can improve prediction accuracy and treatment efficacy, which is critically important in the dynamically evolving field of transplantation.Objective. The aim of this study is to develop a prognostic model for liver transplantation outcomes in patients with hepatocellular carcinoma and liver cirrhosis.Material and methods. This retrospective study included 69 patients with hepatocellular carcinoma on the background of liver cirrhosis who underwent liver transplantation between May 2010 and December 2022. Of these, 42 patients (61%) received organs from living donors, and 27 (39%) from deceased donors. The study involved analysis of alpha-fetoprotein levels in blood, as well as assessment of radiological (maximum tumor nodule size, number of nodules) and histological parameters (maximum tumor nodule size, number of nodules, presence of vascular invasion). Cox regression model was used to predict recurrence-free survival, and the results for five-year recurrence-free survival, recipient age, and donor type were reused in the Cox model to predict overall survival.Results. Four models for predicting recurrence-free survival and overall survival based on histological and radiological data were developed, demonstrating high prognostic value with C-indexes on training/test data of 0.76/1; 0.73/1; 0.78/0.8; 0.6/0.8 respectively. All models showed recurrence-free survival prediction accuracy comparable to the Milan criteria. The model outcomes are available as a calculator on the website https://nadit.ru/calculate_HCC.Conclusion. The developed prognostic models are vital tools for personalized outcome prediction after liver transplantation for hepatocellular carcinoma. To enhance the accuracy of these models, further amalgamation and validation of data from various medical centers, as well as open scientific collaboration, are necessary.
The effect of vibroacoustic therapy on the structural integrity of erythrocytes and platelets in patients with acute exogenous poisoning
Authors: Makarov M. S. , Goldfarb Yu. S. , Badalyan A. V. , Simonova A. Yu. , Potskhveriya M. M. 
Published: September 2024

Background. Vibroacoustic therapy is used as a non-drug method of correcting respiratory disorders in various diseases, including acute poisoning. The effect of vibroacoustic therapy on blood cells currently remains unexplored.Objective. To evaluate the impact of vibroacoustic therapy on the morphofunctional rate of erythrocytes and platelets in patients with acute poisoning in patients with acute poisoning.Material and methods. Thirty eight patients with complicated acute poisoning transferred to the Toxicology Department of the N.V. Sklifosovsky Research Institute for Emergency Medicine were examined. In 19 patients, along with the standard therapy, a vibroacoustic therapy was performed using a VibroLUNG device (the main study group); the other 19 patients received only standard therapy (the comparison group). Vibroacoustic therapy began on the 2nd day after the patient admission to the Toxicology Department. The course of vibroacoustic therapy consisted of 2–9 procedures. Morphofunctional analysis of erythrocytes and platelets in all cases was performed before the start of the vibroacoustic therapy (before treatment) and before discharge from the hospital (1–2 days after completing the vibroacoustic therapy course).Results. In both groups, before treatment, we noted normal morphofunctional rate of erythrocytes and normal level of platelets with granules in the circulating blood. The level of severely damaged or altered platelets was only slightly higher than normal and did not differ between the groups. Meanwhile, in 50% of patients, platelets had a tendency to spontaneous activation and hyperactivation. Before discharge, the morph functional parameters of erythrocytes and platelets did not significantly change in both groups, while treatment with vibroacoustic therapy allowed to eliminate or reduce spontaneous platelet activation in 7 of 9 patients in the main study group. In the comparison group spontaneous platelet activation was absent in only 3 of 10 patients. In parallel, the time till pneumonia resolution turned statistically significantly reduced, by 1.5 times.Conclusions. In acute exogenous poisoning with complicated course, the risk of spontaneous platelet activation and hyperactivation increases. Vibroacoustic therapy does not violate the structural integrity of circulating erythrocytes and platelets. Vibroacoustic therapy, using the VibroLUNG device, reduces the tendency of platelets to spontaneous activation and hyperactivation, reduces the time for resolving pneumonia.
Treatment of recurrent ureteral stricture after kidney transplantation with nitinol stent
Authors: Shabunin A. V. , Chechenin G. M. , Drozdov P. A. , Nesterenko I. V. , Astapovich S. A. , Lidzhieva E. A. 
Published: September 2024

Introduction. Urological complications make up a significant percentage in the structure of kidney graft loss and patient death in the early and long-term period after transplantation. The most common urological complication in the long-term period is ureter or anastomotic stricture, which, according to various authors, occurs in 0.9–34%. However, now there is no consensus in the treatment for recurrent strictures.Case report. We have presented a clinical case of successful treatment for the stricture of the ureterovesical anastomosis after kidney transplant from a brain-dead donor. During the first year after kidney transplantation, after two reconstructive surgeries and repeated placement of plastic stents, the patient was diagnosed with recurrent stricture of the ureterovesical anastomosis, and therefore a coated nitinol ureteral stent was implanted.Conclusion. This clinical case report demonstrates the feasibility of effectively using a nitinol stent in the treatment of recurrent strictures of the ureterovesical anastomosis after kidney transplantation. In some cases, this technique can be considered as an alternative to repeated surgical interventions. Further studies are needed to determine a more precise treatment algorithm.
A robust septic hermite collocation technique for dirichlet boundary condition Heat conduction equation
Authors: Kutluay Selçuk , Yağmurlu Nuri Murat , Karakaş Ali Sercan 
Published: September 2024

Abstract
In the current manuscript, approximate solution for 1D heat conduction equation will be sought with the Septic Hermite Collocation Method (SHCM). To achieve this goal, by means of the roots of both Chebyschev and Legendre polynomials used at the inner collocation points, the pseudo code of the method is found out and applied using Matlab which is one of the widely utilized symbolic programming platforms. The unconditional stability of the scheme is shown by the traditional von-Neumann stability technique. To illustrate the accuracy and effectiveness of this newly current numerical scheme, a comparison among analytical and the computed numerical results is presented in tabular forms. It has been illustrated that the scheme is both accurate and effective one and at the same time can be used in a successful way for finding out numerical solutions of several nonlinear problems as well as linear ones.