bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and Molecular Characteristics of High-Risk, Recurrent, or Metastatic Endometrial Cancer That Is Human Epidermal Growth Factor Receptor 2–Low
Authors: van Dijk Dione , Vermij Lisa , León-Castillo Alicia , Powell Melanie , Jobsen Jan , Leary Alexandra , Bowes David , Mileshkin Linda , Genestie Catherine , Jürgenliemk-Schulz Ina , de Kroon Cor , Post Cathelijne , de Boer Stephanie , Nooij Linda , Kroep Judith , Creutzberg Carien , Smit Vincent , Horeweg Nanda , Bosse Tjalling , Westermann Anneke 
Published: October 2024

PURPOSE
Recent success of human epidermal growth factor receptor 2 (HER2)–targeted antibody-drug-conjugate trastuzumab-deruxtecan in HER2-low and HER2-positive tumors has sparked interest in examining the HER2 status of tumors not traditionally associated with HER2 amplification. Despite the increasing number of systemic treatment options, patients with advanced endometrial cancer (EC) still face a poor prognosis. This study evaluates HER2-low status in over 800 EC, correlating HER2 with both molecular and clinical features.

METHODS
HER2 status was determined by immunohistochemistry (IHC) and dual in situ hybridization (DISH) on four studies of previously classified high-risk EC (PORTEC-3 and Medical Spectrum Twente cohort), recurrent or metastatic EC (DOMEC), and a primary stage IV cohort. EC was classified as HER2-negative (IHC 0), HER2-low (IHC 1+/2+ without amplification), or HER2-positive (IHC 3+ or DISH-confirmed amplification). Survival analysis was performed using the Kaplan-Meier method. Cox proportional hazards models assessed the independence of any prognostic impact of HER2 status.

RESULTS
HER2 status was determined in 806 EC: 74.8% were HER2-negative, 17.2% HER2-low, and 7.9% HER2-positive. HER2-low was found across all molecular classes and histotypes. The highest rates of HER2-low and HER2-positive tumors were in recurrent or metastatic EC (35.6% and 15.6%), followed by primary stage IV EC (29.9% and 12.4%) and high-risk EC (14.2% and 6.8%). HER2 status had no independent prognostic value.

CONCLUSION
A quarter of high-risk, metastatic, or recurrent EC exhibited HER2 overexpression. The presence of HER2 overexpression in all clinical and molecular categories highlights the need for broad testing and offers treatment options for a wide range of patients.
Safety of In-hospital Parenteral Antiosteoporosis Therapy Following a Hip Fracture: A Retrospective Cohort
Authors: Abu-Jwead Alaa , Fisher David L , Goldabart Adi , Yoel Uri , Press Yan , Tsur Anat , Fraenkel Merav , Baraf Lior 
Published: October 2024

Abstract

Purpose
To assess the safety of zoledronic acid (ZOL) and denosumab (Dmab) administered following hip fracture in a hospital setting.

Methods
Patients older than 65 years were treated by a fracture liaison service following hip fracture. Generally, patients who had a glomerular filtration rate (eGFR) &gt; 35 mL/min were treated with ZOL, whereas patients who had previously received bisphosphonates or had a eGFR between 20 and 35 mL/min were treated with Dmab. Adverse events included hypocalcemia (calcium corrected for albumin less than 8.5 mg/day), renal functional impairment (0.5 mg/dL or more increase in serum creatinine) within 30 days of treatment, or a fever (&gt;38 °C) within 48 hours of drug administration.

Results
Two hundred twenty-eight and 134 patients were treated with ZOL and Dmab, respectively. Mean body temperature was elevated following ZOL administration (0.18 °C P &lt; .001) but remained below 38 °C. Hypocalcemia occurred in 18% and 29% of the ZOL and Dmab groups, respectively (P = .009). Renal functional impairment was observed in 9 and 6 patients (4% and 5%) in the ZOL and Dmab groups, respectively (P = .8). Pretreatment calcium above 9.3 mg/dL was associated with a lower risk of posttreatment hypocalcemia (odds ratio 0.30, 95% confidence interval 0.13-0.68, P = .004). While the absolute risk of hypocalcemia was higher in the Dmab group, multivariate analysis did not find that the choice of drug was predictive of hypocalcemia.

Conclusion
In-hospital parenteral osteoporosis treatment was rarely associated with fever or renal function impairment but was associated with hypocalcemia. Posttreatment hypocalcemia risk did not vary significantly between patients receiving ZOL or Dmab.
The evolution of multicellularity and cell differentiation symposium: bridging evolutionary cell biology and computational modelling using emerging model systems
Authors: Ros-Rocher Núria 
Published: October 2024

ABSTRACT
‘The evolution of multicellularity and cell differentiation’ symposium, organized as part of the EuroEvoDevo 2024 meeting on June 25-28th in Helsinki (Finland), addressed recent advances on the molecular and mechanistic basis for the evolution of multicellularity and cell differentiation in eukaryotes. The symposium involved over 100 participants and brought together 10 speakers at diverse career stages. Talks covered various topics at the interface of developmental biology, evolutionary cell biology, comparative genomics, computational biology, and ecology using animal, protist, algal and mathematical models. This symposium offered a unique opportunity for interdisciplinary dialog among researchers working on different systems, especially in promoting collaborations and aligning strategies for studying emerging model species. Moreover, it fostered opportunities to promote early career researchers in the field and opened discussions of ongoing work and unpublished results. In this Meeting Review, we aim to promote the research, capture the spirit of the meeting, and present key topics discussed within this dynamic, growing and open community.
C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair
Authors: Pooranachithra Murugesan , Jyo Erin M. , Brouilly Nicolas , Pujol Nathalie , Ernst Andreas M. , Chisholm Andrew D. 
Published: October 2024

ABSTRACT
The apical extracellular matrix (aECM) of external epithelia often contains lipid-rich outer layers that contribute to permeability barrier function. The external aECM of nematodes is known as the cuticle and contains an external lipid-rich layer – the epicuticle. Epicuticlins are a family of tandem repeat cuticle proteins of unknown function. Here, we analyze the localization and function of the three C. elegans epicuticlins (EPIC proteins). EPIC-1 and EPIC-2 localize to the surface of the cuticle near the outer lipid layer, as well as to interfacial cuticles and adult-specific struts. EPIC-3 is expressed in dauer larvae and localizes to interfacial aECM in the buccal cavity. Skin wounding in the adult induces epic-3 expression, and EPIC proteins localize to wound sites. Null mutants lacking EPIC proteins are viable with reduced permeability barrier function and normal epicuticle lipid mobility. Loss of function in EPIC genes modifies the skin blistering phenotypes of Bli mutants and reduces survival after skin wounding. Our results suggest EPIC proteins define specific cortical compartments of the aECM and promote wound repair.
Optimized methods to image hepatic lipid droplets in zebrafish larvae
Authors: Khan Nouf , Mohd Salmi Talhah , Karamalakis Anthony P. , Ramdas Nair Anjana , Sadler Kirsten C. , Cox Andrew G. 
Published: October 2024

ABSTRACT
The optical transparency of zebrafish larvae enables visualization of subcellular structures in intact organs, and these vertebrates are widely used to study lipid biology and liver disease. Lipid droplet (LD) presence is a prevalent feature of healthy cells, but, under conditions such as nutrient excess, toxicant exposure or metabolic imbalance, LD accumulation in hepatocytes can be a harbinger of more severe forms of liver disease. We undertook a comprehensive analysis of approaches useful to investigate LD distribution and dynamics in physiological and pathological conditions in the liver of zebrafish larvae. This comparative analysis of the lipid dyes Oil Red O, Nile Red, LipidTox and LipidSpot, as well as transgenic LD reporters that rely on EGFP fusions of the LD-decorating protein perilipin 2 (PLIN2), demonstrates the strengths and limitations of each approach. These protocols are amenable to detection methods ranging from low-resolution stereomicroscopy to confocal imaging, which enables measurements of hepatic LD size, number and dynamics at cellular resolution in live and fixed animals. This resource will benefit investigators studying LD biology in zebrafish disease models.
The lysosomal lipid transporter LIMP-2 is part of lysosome–ER STARD3-VAPB-dependent contact sites
Authors: Rudnik Sönke , Heybrock Saskia , Coyaud Etienne , Xu Zizhen , Neculai Dante , Raught Brian , Oorschot Viola , Heus Cecilia , Klumperman Judith , Saftig Paul 
Published: October 2024

ABSTRACT
LIMP-2 (also known as SCARB2) is an abundant lysosomal membrane protein. Previous studies have shown that LIMP-2 functions as a virus receptor, a chaperone for lysosomal enzyme targeting and a lipid transporter. The large luminal domain of LIMP-2 contains a hydrophobic tunnel that enables transport of phospholipids, sphingosine and cholesterol from the lysosomal lumen to the membrane. The question about the fate of the lipids after LIMP-2-mediated transport is largely unexplored. To elucidate whether LIMP-2 is present at contact sites between lysosomes and the endoplasmic reticulum (ER), we performed a proximity-based interaction screen. This revealed that LIMP-2 interacts with the endosomal protein STARD3 and the ER-resident protein VAPB. Using imaging and co-immunoprecipitation, we demonstrated colocalization and physical interaction between LIMP-2 and these proteins. Moreover, we found that interaction of LIMP-2 with VAPB required the presence of STARD3. Our findings suggest that LIMP-2 is present at ER–lysosome contact sites, possibly facilitating cholesterol transport from the lysosomal to the ER membrane. This suggests a novel mechanism for inter-organelle communication and lipid trafficking mediated by LIMP-2.
Figure Skating Musculoskeletal Injury: Evidence across Disciplines, Mechanisms, and Future Directions
Authors: Madsen Aimee , Alfonso Kathryn , Vincent Heather K. 
Published: October 2024

Abstract
This review provides a synopsis of current injury patterns, potential mechanisms, and off-ice exercise prevention considerations as the sport of figure skating evolves. The lifetime prevalence of injury for all skaters is estimated to be 79.5%, with the most common sites being spine and lower extremity. Singles skaters more often develop chronic injuries compared to acute (68.9% vs 31.1%), whereas non-singles skaters more often experience acute injuries. Lower limb stress fracture prevalence ranges from 10% to 21.4% across disciplines. Emerging contributors include training volume, technical difficulty and repetition of complex elements (multirotational jumps, lifts, holds), extreme ranges of movement about the spine and hip, age and experience level, and functional capacity (dynamic strength, flexibility, balance, and neuromotor control). Prevention programs in competitive figure skaters may include monitoring of training volumes, emphasis on technique, appropriate boot fit, and strength and conditioning content to prepare for increasing neuromotor, balance, and power demands of the sport.
Structural design and cryogenic stability analysis for long-wave infrared spatial heterodyne spectrometers
Authors: Wu Yang , Feng Yutao , Han Bin , Wu Junqiang , Sun Jian 
Published: October 2024

Under cryogenic conditions, long-wave infrared spatial heterodyne spectrometers (LWIR-SHSs) may exhibit variations in the inclination, frequency, and distortion of their interference fringes, which can affect the system’s performance. To address the stability issues associated with cryogenics, an analysis and simulation of the optomechanical system’s performance were conducted under such conditions. Employing a structural thermal optical performance (STOP) analysis, an integrated micro-stress dynamic flexible support structure for the LWIR-SHS was designed. The optimized system ensured stable operation under cryogenic conditions, maintaining the relative positional deviation of the key components below 0.00146 mm, the relative angular deviation within 25.984′′, and the inclination of interference fringes in both simulation and tests less than 21°. Furthermore, in comparison to the initial system, the interference fringe distortion also decreased significantly. Cryogenic testing of the entire system validated the optimized structure’s effectiveness in minimizing variations of the interferogram, aligning with simulation predictions. This outcome demonstrates the optimization strategy’s substantial impact on enhancing both the structural stability and operational performance of the cryogenic infrared optical system.
Spatiotemporal dissipation dynamics: a route to high-beam-quality and high-peak-power spatiotemporal mode-locked fiber lasers
Authors: Fu Guohao , Li Yuhang , Xiao Qirong , Li Dan , Gong Mali , Liu Qiang , Wabnitz Stefan , Yan Ping 
Published: October 2024

Spatiotemporal mode-locking (STML) opens a new avenue for implementing high-energy, high-peak-power mode-locked fiber oscillators. However, the compromised beam quality poses a critical limitation to their broader applications. This study presents a method for enhancing the beam quality of STML fiber lasers by employing spatiotemporal dissipation involving the quenching and reabsorption effects of multimode erbium-doped fibers. The proposed technique introduces spatiotemporal saturable absorption, achieving high beam quality without the stringent conditions required for Kerr beam self-cleaning (BSC). Integrating spatiotemporal dissipation with Kerr BSC, we demonstrate an all-anomalous-dispersion Er-doped STML fiber laser, which produces solitons with 6.7 nJ pulse energy (the intracavity solitons with 25.8 nJ pulse energy and >52.8kW peak power), sub-500 fs pulse duration, and beam quality with M
x
2/M
y
2=1.23/1.20. To our knowledge, it is a record peak power for 1.5 µm band soliton lasers. Additionally, the approach enables the generation of noise-like pulses with M
x
2/M
y
2=1.04/1.13. This work not only advances our understanding of spatiotemporal dissipation dynamics in STML fiber lasers, but also paves the way toward high-performance STML fiber lasers, rendering them very attractive for applications.
The self-correction and influence factors of congenital auricular deformity: A prospective cohort study
Authors: Huang Jincheng , Zou Kun , Yang Min , Fan Yanjun , Xia Jinjie , Zhao Li 
Published: October 2024

Objective
To prospectively observe the self-correction of congenital auricular deformity (CAD) and explore the potential factors affecting the self-correction.

Methods
This study was a multi-center prospective observational study. Newborns aged 0–3 days from 12 Maternal and Child Health Hospitals or Maternity Hospitals were chosen as the participants and prospectively followed up until week 6 after birth. The primary and secondary outcome was the score of deformity, and the secondary outcome was the improvement rate, respectively.

Results
A total of 135 newborns diagnosed with CAD (237 ears) were recruited. Boys and girls accounted for 50.37% (117 ears) and 49.63% (120 ears). The top morphological type was the constricted ear (107 ears, 45.15%). The score of deformity at baseline, week 3, and week 6 after enrollment was 4.00, 3.00, and 2.00, decreasing over time (P < 0.05). The higher the severity of deformity, the worse the self-correcting effect (P < 0.05). The scores of deformity of Stahl’s ear were lower than those of others after follow-up (P < 0.05). No significant differences among the scores of deformity in different genders (P >0.05). The total improvement rate at week 3 and week 6 was 29.96% (71/237 ears) and 37.13% (88/237), respectively. The improvement rate of the Stahl’s ear at week 3 and week 6 after enrollment was higher than that of four other morphological types (P < 0.05).

Conclusions
Some CAD tends to self-correction, but for most CADs, there is still a need for early correction. Morphological types and severity of deformity are the main influencing factors on self-correcting effect, whereas sex was not.
Evaluation of the unavailability of the primary circuit of Triga SSR reactor, importance factors and risk criteria for its components
Authors: Morlova Elena D. , Mladin Daniela 
Published: October 2024

Abstract
Probabilistic Safety Assessment is a tool for improving a nuclear installation and its safety in all its function phases. PSA provides a methodical way of identifying sequences resulting from a wide range of initiating events concerning an accident, and includes systematic and realistic determination of accident frequencies and their consequences. This paper analyzes the unavailability of the TRIGA SSR reactor primary circuit, obtaining the system unavailability value, identifying main contributors to system failure, and calculating risk importance factors. Two cases were considered: one without considering Common Cause Failure (CCF) in the developed fault tree, and the second considering CCF on pumps and heat exchangers lines. The aim was to assess their influence on the primary system’s unavailability. Two importance factors, Fussell–Vesely and Risk Achievement Worth, were employed to identify components with a strong impact on the risk (probability of system failure). These factors aid in enhancing maintenance activities and testing. The analysis utilized the EDFT code, part of the PSAMAN code package developed in SCN-Pitesti.
Ambient Temporalities: Rethinking Object-Oriented Time through Kant, Husserl, and Heidegger
Authors: Stephenson Jamie 
Published: October 2024

Abstract
Immanuel Kant is often conveyed as a Platonic or Newtonian thinker of the temporal, expressing time as an absolute and continuous repository wherein all objects occur. However, employing themes from his aesthetic writings, what happens when Kantian “sublime” time is reoriented towards a more discontinuous temporal register? This essay employs just such a reading, while also utilising Graham Harman’s Object-Oriented Ontology (OOO), as a methodological device for rethinking both Kantian and object time as neither solely continuous nor discontinuous, but somewhere inbetween these two determinations, in what I term their “ontological ambience.” By doing so I offer a critique of both Kantian orthodoxy and Harmanian OOO, via a comparative analysis of (1) how Edmund Husserl and Martin Heidegger thematise Kantian temporality, and (2) how Harman develops their subsequent ideas about time. This schema provides post-Kantian philosophy with a provisional model for thinking posthuman time in new and productive ways: as ambient temporalities.
Hierarchical Bayesian Modelling of Macroeconomic Variables in Ghana
Authors: Koranteng Emmanuel Amoako , Engmann Gideon Mensah , Jakperik Dioggban 
Published: October 2024

Abstract
This study analyzed the impact of macroeconomic variables (manufacturing, real exchange rate, government expenditure, and gross fixed capital formation) on GDP growth in Ghana. Utilizing secondary data from the World Development Indicators of the World Bank (1991–2021), we employed a hierarchical Bayesian linear model with interaction effects to assess these relationships. The results indicate that the real exchange rate, manufacturing, and government expenditure have a positive influence on GDP growth, while gross fixed capital formation exhibits a moderately negative effect. To enhance economic growth, it is crucial to optimize capital investments, bolster export competitiveness through targeted policies, and invest in manufacturing innovation. These findings offer actionable insights for policymakers aiming to stimulate economic growth in Ghana.
Complete remission after glucocorticoid therapy in a patient with primary hypophysitis
Authors: Hacioglu Aysa , Ekinci Gazanfer , Karaca Zuleyha , Türe Uğur , Kelestimur Fahrettin 
Published: October 2024

Summary
Primary hypophysitis is a rare disease that may have variable clinical presentations. The main treatment options are clinical observation, immunosuppressive drugs, and surgery. Glucocorticoids are used as first-line medical therapy; however, non-responsiveness and recurrences are the major problems. We present a 30-year-old male patient who had an excellent radiologic response to a single course of glucocorticoids. The patient presented with malaise and severe headaches of acute onset. Cranial MRI revealed a pituitary mass compressing the optic chiasm. Hormonal evaluation studies were consistent with anterior pituitary hormone dysfunction except for the growth hormone axis. There was a mild compression on the optic chiasm in the pituitary MRI. The patient was started on methylprednisolone therapy at a dose of 80 mg/day. The pituitary MRI revealed complete regression of the mass after 2 months, and there was a complete recovery of pituitary functions after 6 months. There is no consensus on the optimal dose and duration of glucocorticoid therapy for primary hypophysitis in the literature. We report that steroid therapy, even in lower doses, might be effective in mild-to-moderate cases.

Learning points

Primary hypophysitis is a rare disease with a varied clinical course, and hence the treatment strategies should be individualized.

There is no consensus on the optimal dose and duration of glucocorticoid therapy.

Glucocorticoid therapy may induce complete remission in some patients, especially with a mild-to-moderate disease course and during the acute phase of the disease.
IMPACT OF REAL-LIFE ENVIRONMENTAL EXPOSURES ON REPRODUCTION: Rodent reproductive behavior among males and females after exposure to endocrine-disrupting chemicals
Authors: Maxon Jacob R , Mahoney Megan M 
Published: October 2024

In brief
The rodent reproductive behavioral control column of the hypothalamus and the hypothalamic-pituitary-gonadal (HPG) axis are critical systems that regulate copulatory behaviors in both males and females. We review how endocrine-disrupting chemicals (EDCs), specifically phthalates, bisphenol A, and chemical mixtures, dysregulate appetitive and consummatory copulatory behaviors and their neuroendocrine substrates, using mouse and rodent models.

Abstract
Sexual reproduction – from both physiological and behavioral perspectives – is dependent upon appropriate connections between a diverse, hormone-modulated network of neural regions. Importantly, these substrates are regulated by hormones across the lifespan from early development to adulthood, making them targets of EDCs. Rodents, such as mice and rats, are invaluable to the characterization of EDCs because of their sex-specific, stereotyped appetitive and consummatory reproductive behaviors. Phthalates, bisphenol A (BPA), and EDC mixtures pose a salient risk to the health of humans, wildlife, and livestock because these synthetic compounds are ubiquitous due to their widespread use in the mass production of consumer and industrial goods. This review outlines how the HPG axis regulates male and female sexual behaviors, and how phthalates and BPA can perturb appetitive and consummatory behaviors and impact neural substrates that modulate reproductive behavior. We will then discuss how to progress toward a clearer understanding of the reproductive and neurobiological changes that occur due to EDC exposure.