bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clofarabine versus fludarabine‐based reduced‐intensity conditioning regimen prior to allogeneic transplantation in adults with AML/MDS
Authors: Chevallier Patrice , Labopin Myriam , de La Tour Regis Peffault , Lioure Bruno , Bulabois Claude‐Eric , Huynh Anne , Blaise Didier , Turlure Pascal , Daguindau Etienne , Maillard Natacha , Yakoub‐Agha Ibrahim , Guillerm Gaelle , Delage Jeremy , Contentin Nathalie , Bay Jacques‐Olivier , Beckerich Florence , Bourhis Jean‐Henri , Detrait Marie , Vigouroux Stéphane , François Sylvie , Legrand Faezeh , Guillaume Thierry , Mohty Mohamad ,   the SFGM‐TC
Published: October 2016

AbstractWe have retrospectively compared survivals between acute myeloid leukemia (AML)/myelodysplastic syndrome (MDS) patients who received either a clofarabine/busulfan (CloB2A2) or a fludarabine/busulfan (FB2A2) RIC regimen for allogeneic stem cell transplantation. Between 2009 and 2014, 355 allotransplanted cases were identified from the SFGM‐TC registry as having received either the FB2A2 (n = 316, 56% males, median age: 59.2 years, AML 78.5%, first complete remission [CR1] 72%, median follow‐up: 20 months) or the CloB2A2 (n = 39, 62% males, median age: 60.8 years, AML 62%, CR1 69%, median follow‐up: 22.4 months) RIC regimen. In multivariate analysis, FB2A2 was associated with significant lower overall survival (OS, HR: 2.14; 95%CI: 1.05–4.35, P = 0.04) and higher relapse incidence (RI, HR: 2.17; 95%CI: 1.02–4.61, P = 0.04) and a trend for lower leukemia‐free survival (LFS, HR: 1.75; 95%CI: 0.94–3.26, P = 0.08). These results were confirmed using a propensity score‐matching strategy. However, when considering AML and MDS patients separately, the benefit of the CLOB2A2 regimen was restricted to AML patients (2‐year OS FB2A2: 38% [14.5–61.6] vs. CloB2A2: 79.2% [62.9–95.4], P = 0.01; 2‐year LFS FB2A2: 38% [16–59.9] vs. CloB2A2: 70.8% [52.6–89], P = 0.03). The better survivals were due to the lower risk of relapse in this CloB2A2 AML subgroup (2‐year RI FB2A2: 41.2% [19–62.4] vs. CloB2A2: 16.7% [5–34.2], P = 0.05). This retrospective comparison suggests that the CloB2A2 RIC regimen can likely provide longer survival than that awarded by a FB2A2 RIC regimen and may become a new standard of care RIC regimen for allotransplanted AML patients. A prospective phase 3 randomized study is warranted.
Variants in autophagy‐related genes and clinical characteristics in melanoma: a population‐based study
Authors: White Kirsten A. M. , Luo Li , Thompson Todd A. , Torres Salina , Hu Chien‐An Andy , Thomas Nancy E. , Lilyquist Jenna , Anton‐Culver Hoda , Gruber Stephen B. , From Lynn , Busam Klaus J. , Orlow Irene , Kanetsky Peter A. , Marrett Loraine D. , Gallagher Richard P. , Sacchetto Lidia , Rosso Stefano , Dwyer Terence , Cust Anne E. , Begg Colin B. , Berwick Marianne ,   The GEM Study Group
Published: October 2016

AbstractAutophagy has been linked with melanoma risk and survival, but no polymorphisms in autophagy‐related (ATG) genes have been investigated in relation to melanoma progression. We examined five single‐nucleotide polymorphisms (SNPs) in three ATG genes (ATG5; ATG10; and ATG16L) with known or suspected impact on autophagic flux in an international population‐based case–control study of melanoma. DNA from 911 melanoma patients was genotyped. An association was identified between (GG) (rs2241880) and earlier stage at diagnosis (OR 0.47; 95% Confidence Intervals (CI) = 0.27–0.81, P = 0.02) and a decrease in Breslow thickness (P = 0.03). The ATG16L heterozygous genotype (AG) (rs2241880) was associated with younger age at diagnosis (P = 0.02). Two SNPs in ATG5 were found to be associated with increased stage (rs2245214 CG, OR 1.47; 95% CI = 1.11–1.94, P = 0.03; rs510432 CC, OR 1.84; 95% CI = 1.12–3.02, P = 0.05). Finally, we identified inverse associations between ATG5 (GG rs2245214) and melanomas on the scalp or neck (OR 0.20, 95% CI = 0.05–0.86, P = 0.03); ATG10 (CC) (rs1864182) and brisk tumor infiltrating lymphocytes (TILs) (OR 0.42; 95% CI = 0.21–0.88, P = 0.02), and ATG5 (CC) (rs510432) with nonbrisk TILs (OR 0.55; 95% CI = 0.34–0.87, P = 0.01). Our data suggest that ATG SNPs might be differentially associated with specific host and tumor characteristics including age at diagnosis, TILs, and stage. These associations may be critical to understanding the role of autophagy in cancer, and further investigation will help characterize the contribution of these variants to melanoma progression.
Irregular vascular pattern by contrast‐enhanced ultrasonography and high serum Lens culinaris agglutinin‐reactive fraction of alpha‐fetoprotein level predict poor outcome after successful radiofrequency ablation in patients with early‐stage hepatocellular carcinoma
Authors: Takada Hitomi , Tsuchiya Kaoru , Yasui Yutaka , Nakakuki Natsuko , Tamaki Nobuharu , Suzuki Shoko , Nakanishi Hiroyuki , Itakura Jun , Takahashi Yuka , Kurosaki Masayuki , Asahina Yasuhiro , Enomoto Nobuyuki , Izumi Namiki 
Published: October 2016

AbstractRadiofrequency ablation (RFA) is considered the most effective treatment for early‐stage hepatocellular carcinoma (HCC) patients unsuitable for resection. However, poor outcome after RFA has occasionally been reported worldwide. To predict such an outcome, we investigated imaging findings using contrast‐enhanced ultrasonography (CEUS) with Sonazoid and serum tumor markers before RFA. This study included 176 early‐stage HCC patients who had initially achieved successful RFA. Patients were examined using CEUS; their levels of alpha‐fetoprotein (AFP), Lens culinaris agglutinin‐reactive fraction of AFP (AFP‐L3), and des‐gamma‐carboxy prothrombin before RFA were measured. Sonazoid provided parenchyma‐specific contrast imaging and facilitated tumor vascular architecture imaging through maximum intensity projection (MIP). Kaplan–Meier analysis examined cumulative rates of local tumor progression, intrasubsegmental recurrence, and survival; factors associated with these were determined with Cox proportional hazards analysis. Local tumor progression (n = 15), intrasubsegmental recurrence (n = 46), and death (n = 18) were observed. Irregular pattern in MIP classification and serum AFP‐L3 level (>10%) before RFA were identified as independent risk factors for local tumor progression and intrasubsegmental recurrence. These two factors were independently associated with poor survival after RFA (irregular pattern in MIP: hazard ratio, (HR) = 8.26; 95% confidence interval, (CI) = 2.24–30.3; P = 0.002 and AFP‐L3 > 10%: HR = 2.94; 95% CI = 1.09–7.94; P = 0.033). Irregular MIP pattern by CEUS and high level of serum AFP‐L3 were independent risk factors for poor outcome after successful RFA. The Patients with these findings should be considered as special high‐risk group in early‐stage HCC.
A case of new‐onset cardiomyopathy and ventricular tachycardia in a patient receiving ibrutinib for relapsed mantle cell lymphoma
Authors: Wallace Natalie , Wong Ellice , Cooper Dennis , Chao Herta 
Published: October 2016

Key Clinical MessageIbrutinib is a first‐in‐class inhibitor of Bruton's tyrosine kinase, which is approved for use in chronic lymphocytic leukemia, mantle cell lymphoma, and Waldenstrom's macroglobulinemia. Although ibrutinib has been linked to an increased incidence of atrial fibrillation, this is the first report of an association with nonischemic cardiomyopathy and ventricular arrhythmia.
Density Functional Determination of the Energetics of the Formation of trans‐Stilbene Catalyzed by Sulfenate Anions
Authors: De Raffele Daria , Piazzetta Paolo , Russo Nino , Toscano Marirosa , Adamo Carlo 
Published: October 2016

Abstracttrans‐Stilbene is an important building block of useful materials such as dyes, fluorescent whiteners, liquid crystals, pigments, optical brighteners, scintillators, and organic light‐emitting diodes. Taking cue from an innovative study devoted to the synthesis of these systems, we explored, through a density functional theory investigation, the mechanism of the conversion of benzyl chloride into trans‐stilbene catalyzed by sulfenate anions; we examined all steps of the reaction to rationalize the experimental findings through determination of both the thermodynamic and kinetic features.
Reactive Oxygen Species Release, Alkylating Ability, and DNA Interactions of a Pterocarpanquinone: A Test Case for Electrochemistry
Authors: Silva Thaissa L. , Ferreira Fabricia R. , de Vasconcelos Camila C. , da Silva Rosanny Christhinny , Lima Dimas José da P. , Costa Paulo R. R. , Netto Chaquip D. , Goulart Marília O. F. 
Published: October 2016

AbstractThe electrochemistry of a redox‐based bioactive pterocarpanquinone, designated as LQB‐118, and its precursor chromenquinone (CQ) was investigated in protic and aprotic media with a focus on the reduction mechanism in both media, the reactivity with oxygen, and the interaction with biological targets, such as DNA. UV/Vis spectroelectrochemistry clarified the proposed mechanism. The appearance of bands at λ=331, 400, and 600 nm suggests the generation of transient quinonemethides (QM). Electrochemical experiments revealed homogeneous electron transfer to oxygen. LQB‐118 itself interacts with calf‐thymus DNA and ssDNA in solution. It, and its electrogenerated intermediates, have been shown to decrease the diagnostic oxidation peaks of guanosine and adenosine residues. These results, together with electrochemical evidence for the formation of QMs and the reductive addition of thiols, partly explain the reported cytotoxic and parasiticidal effects of this quinone. Overall, the electrochemical methods do well to predict the molecular mechanism of the biological activity of the present class of compounds. As an additional competitive advantage, electrochemistry allows reductive cleavage in situ, the characterization of the generated intermediate, the calculation of the number of transferred electrons, and positively mimics in vitro and in vivo experiments.
Optimal Bicelle Size q for Solution NMR Studies of the Protein Transmembrane Partition
Authors: Piai Alessandro , Fu Qingshan , Dev Jyoti , Chou James J. 
Published: October 2016

AbstractStructural characterization of transmembrane proteins in isotropic bicelles has become an increasingly popular application of solution NMR spectroscopy, as the fast‐tumbling bicelles are membrane‐like, yet can often yield spectral quality comparable to those of detergent micelles. While larger bicelles are closer to the true lipid bilayer, it remains unclear how large the bicelles need to be to allow accurate assessment of the protein transmembrane partition in the lipid bilayer. Here, we address the above question from the perspective of the protein residing in the bicelles, through systematic measurement of the protein chemical shift and transmembrane partition at different lipid/detergent ratios (q), ranging from 0.3 to 0.7, using the transmembrane domain of the human Fas receptor as model system. We found that the lipid environment of the bicelles, as reflected by the protein chemical shift, begins to be perturbed when q is reduced to below 0.6. We also implemented a solvent paramagnetic relaxation enhancement (PRE) approach for bicelles to show that the protein transmembrane partition in bicelles with q=0.5 and 0.7 are very similar, but at q=0.3 the solvent PRE profile is significantly different. Our data indicate that q values between 0.5 and 0.6 are a good compromise between high resolution NMR and closeness to the membrane environment, and allow accurate characterization of the protein position in the lipid bilayer.
The Brønsted Acid‐Catalyzed, Enantioselective Aza‐Diels–Alder Reaction for the Direct Synthesis of Chiral Piperidones
Authors: Weilbeer Claudia , Sickert Marcel , Naumov Sergei , Schneider Christoph 
Published: October 2016

AbstractWe disclose herein the first enantioselective aza‐Diels–Alder reaction of β‐alkyl‐substituted vinylketene silyl‐O,O‐acetals and imines furnishing a broad range of optically highly enriched 4‐alkyl‐substituted 2‐piperidones. As a catalyst for this one‐pot reaction we employed a chiral phosphoric acid which effects a vinylogous Mannich reaction directly followed by ring‐closure to the lactam. Subsequent fully diastereoselective transformations including hydrogenation, enolate alkylation, and lactam alkylation/reduction processes converted the cycloadducts into various highly substituted piperidines of great utility for the synthesis of natural products and medicinally active compounds.
Total Synthesis of Gelsedilam by Means of a Thiol‐Mediated Diastereoselective Conjugate Addition–Aldol Reaction
Authors: Huang You‐Ming , Liu Yang , Zheng Chang‐Wu , Jin Qiao‐Wen , Pan Lu , Pan Ren‐Ming , Liu Jun , Zhao Gang 
Published: October 2016

AbstractThe total synthesis of gelsedilam, which features a highly diastereoselective thiol conjugate addition–intramolecular aldol reaction to install the strained and caged [3.2.2] bridged ring system and highly efficient NiCl2/NaBH4‐mediated four‐step transformation in one‐pot to construct its five‐membered lactam ring is reported. The synthesis requires only 18 linear steps from the known compounds, providing useful strategies for the construction of the intricate ring system in the synthesis of related gelsedine‐type alkaloids.
Influence of the Thiol Anchor on the Orientation of Surface‐Grafted dsDNA Assemblies
Authors: Su Qiang , Nöll Gilbert 
Published: October 2016

AbstractThe orientation of surface‐grafted dsDNA assemblies relative to the surface depends strongly on the nature of the employed thiol anchor. This was shown by ssDNA capture probe strands of 20 bases grafted to a gold surface by three dithiane rings or a single mercaptohexyl group. The capture probe strands were hybridized to one end of complementary ssDNA strands (target) comprising 40, 60, or 80 bases (T40, T60, and T80). At the other end of the targets a fluorophore‐labeled reporter probe ssDNA strand of 20 bases was hybridized. To stiffen the DNA assemblies, the targets T60 and T80 were further hybridized to ssDNA patches of 20 or 40 bases. Whether the fluorescence intensity, and thus the distance between surface and fluorophore, increases or decreases with increasing target length depends on the thiol anchor. Attempts were made to heal the nicks that are present in the formed dsDNA assemblies by ligation. For enzymatic ligation, the presence of a phosphate at the 5′‐end of the reporter probe and a patch is required, which may also influence the fluorescence intensity.
Syntheses and Photophysical Properties of meso‐Phenylene ridged Boron Dipyrromethene Monomers, Dimers and Trimer
Authors: Li Tingting , Gu Wei , Yu Changjiang , Lv Xiaokang , Wang Hua , Hao Erhong , Jiao Lijuan 
Published: October 2016

AbstractMultichromophoric boron dipyrromethene (BODIPY) dyes have been efficiently synthesized from a one‐pot condensation of acyl chloride with 2,4‐dimethylpyrrole and have been converted to energy transfer cassettes through a one‐pot Knoevenagel condensation. Various BODIPYs containing 2‐ketopyrrole unit were also isolated as the side products and one of their BF2 complex was also isolated from these condensation reactions. The fluorescence quantum yields of these phenylene bridged dimers and trimer are much lower than those of their corresponding monomers. A significant decrease of the fluorescence quantum yield was observed in most polar solvents for these dimers and trimer, which may be due to a possible symmetry breaking resulted intramolecular charge transfer (ICT) in the excited state. Efficient energy transfer between the donor and acceptor was observed in these energy transfer cassettes, which could be useful as large pseudo‐Stokes shift fluorescent dyes with potential applications in diverse fields.
Sudden death and its risk factors after atrioventricular junction ablation and pacemaker implantation in patients with atrial fibrillation
Authors: Wang Ru‐Xing , Lee Hon‐Chi , Li Jia‐Ping , Hodge David O. , Cha Yong‐Mei , Friedman Paul A. , Munger Thomas M. , Srivathsan Komandoor , Pavri Behzad B. , Shen Win‐Kuang 
Published: October 2016

BackgroundAlthough sudden death (SD) is a rare complication after atrioventricular junction (AVJ) ablation and permanent pacemaker implantation, the risk factors leading to this SD remain unknown. The purpose of this study was to investigate SD and its risk factors after ablate‐and‐pace strategy for rate control in atrial fibrillation (AF) patients during long‐term follow‐up.HypothesisMethodsFrom January 2005 to December 2009, we enrolled into this study 517 AF patients with AVJ ablation and right ventricular pacemaker implantation. Patients were divided into 2 groups, SD and non‐SD. Cox proportional hazards models were used to assess potential risk factors for overall mortality and SD.ResultsDuring a mean follow‐up of 25.8 ± 18.6 months (range, 3 days to 63.8 months), 53 patients died (15 with SD). Cox proportional hazards models showed that the presence of congestive heart failure, New York Heart Association functional class, chronic renal failure, and nonsustained ventricular tachycardia were risk factors that predicted overall mortality. For SD, the presence of dilated cardiomyopathy and mitral stenosis were associated risk factors. SD was exclusively seen in patients who had narrow QRS complex or right bundle branch block prior to AVJ ablation and pacemaker implantation; SD was not seen in any patient with preexisting complete left bundle branch block.ConclusionsDilated cardiomyopathy, mitral stenosis, and baseline QRS morphology should be examined as potential risk factors for SD after AVJ ablation and pacemaker implantation.
Performance and energy efficiency analysis of HPC physics simulation applications in a cluster of ARM processors
Authors: Bez Jean Luca , Bernart Eliezer E. , dos Santos Fernando F. , Schnorr Lucas Mello , Navaux Philippe Olivier Alexandre 
Published: October 2016

SummaryWe analyze the feasibility and energy efficiency of using an unconventional cluster of low‐power Advanced RISC Machines processors to execute two scientific parallel applications. For this purpose, we have selected two applications that present high computational and communication cost: the Ondes3D that simulates geophysical events, and the all‐pairs N‐Body that simulates astrophysical events. We compare and discuss the impact of different compilation directives and processor frequency and how they interfere in Time‐to‐Solution and Energy‐to‐Solution. Our results demonstrate that by correctly tuning the application at compile time, for the Advanced RISC Machines architecture, we can considerably reduce the execution time and the energy spent by computing simulations. Furthermore, we observe reductions of up to 54.14% in Time‐to‐Solution and gains of up to 53.65% in Energy‐to‐Solution with two cores. Additionally, we consider the impact of two processor frequency governors on these metrics. Results indicate that the powersave governor presents a smaller instantaneous power consumption. However, it spends more time executing tasks, increasing the energy needed to achieve the solution. Finally, we correlate the energy consumption with the execution time in the experimental results using Pareto. These findings suggest that it is possible to explore low‐powered clusters for high‐performance computing applications by tuning application and hardware configuration to achieve energy efficiency. Copyright © 2016 John Wiley & Sons, Ltd.
Correlation between the O 2p Orbital and Redox Reaction in LiMn0.6Fe0.4PO4 Nanowires Studied by Soft X‐ray Absorption
Authors: Asakura Daisuke , Hosono Eiji , Okubo Masashi , Nanba Yusuke , Zhou Haoshen , Glans Per‐Anders , Guo Jinghua 
Published: October 2016

AbstractThe changes in the electronic structure of LiMn0.6Fe0.4PO4 nanowires during discharge processes were investigated by using ex situ soft X‐ray absorption spectroscopy. The Fe L‐edge X‐ray absorption spectrum attributes the potential plateau at 3.45 V versus Li/Li+ of the discharge curve to a reduction of Fe3+ to Fe2+. The Mn L‐edge X‐ray absorption spectra exhibit the Mn2+ multiplet structure throughout the discharge process, and the crystal‐field splitting was slightly enhanced upon full discharge. The configuration‐interaction full‐multiplet calculation for the X‐ray absorption spectra reveals that the charge‐transfer effect from O 2p to Mn 3d orbitals should be considerably small, unlike that from the O 2p to Fe 3d orbitals. Instead, the O K‐edge X‐ray absorption spectrum shows a clear spectral change during the discharge process, suggesting that the hybridization of O 2p orbitals with Fe 3d orbitals contributes essentially to the reduction.
A review of subtropical community resistance and resilience to extreme cold spells
Authors: Boucek R. E. , Gaiser E. E. , Liu H. , Rehage J. S. 
Published: October 2016

AbstractForecasted changes in global climate predict not only shifts in average conditions but also changes in the frequency and intensity of climate extremes. In the subtropics, the passage of extreme cold spells functions as a major structuring force for ecological communities, and can incur substantial losses to biodiversity, agriculture, and infrastructure. If these events persist in the future, it is likely that their effects on subtropical communities and ecosystems will become more pronounced, as tropical species migrate poleward. Recent extreme cold spells in subtropical China (2008) and United States (2010) occurred in ecosystems that are the subject of long‐term ecological study, enabling key questions about cold spell affects to be addressed. In this study, we (1) discuss the meteorological drivers that resulted in these two extreme cold spells, and (2) use findings from case studies published in theEcosphereSpecial Feature on effects of extreme cold spells on the dynamics of subtropical communities, and on poleward expansion of tropical species and other previously published works to identify consistencies of subtropical community resilience and resistance to extreme cold spells. In this review, we highlight three consistent findings related to this particularly type of extreme climate event: (1) cold spells drive predictable community change in the subtropics by altering ratios of coexisting tropical and temperate species; (2) certain landscape features consistently affect subtropical resistance and resilience to extreme cold spells; and (3) native tropical species are more resistant and resilient to extreme cold spells than tropical nonnative taxa. Our review should improve forecasts of the response of subtropical community dynamics in scenarios where extreme cold spells either increase or decrease in frequency and intensity.