bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional diversity of oxidosqualene cyclases in genus Oryza
Authors: Ma Aimin , Sun Juncong , Feng Laibao , Xue Zheyong , Wu Wenbin , Song Bo , Xiong Xingchen , Wang Xiaoning , Han Bin , Osbourn Anne , Qi Xiaoquan 
Published: October 2024

Summary
Triterpene skeletons, catalyzing by 2,3‐oxidosqualene cyclases (OSCs), are essential for synthesis of steroids and triterpenoids. In japonica rice cultivars Zhonghua11, a total of 12 OsOSCs have been found. While the catalytic functions of OsOSC1, 3, 4, 9, and 10 remain unclear, the functions of the other OsOSCs have been well studied.
In this study, we conducted a comprehensive analysis of 12 OSC genes within genus Oryza with the aid of 63 genomes from cultivated and wild rice. We found that OSC genes are relatively conserved within genus Oryza with a few exceptions. Collinearity analysis further suggested that, throughout the evolutionary history of genus Oryza, the OSC genes have not undergone significant rearrangements or losses.
Further functional analysis of 5 uncharacterized OSCs revealed that OsOSC10 was a friedelin synthase, which affected the development of rice grains. Additionally, the reconstructed ancestral sequences of Oryza OSC3 and Oryza OSC9 had lupeol synthase and poaceatapetol synthase activity, respectively.
The discovery of friedelin synthase in rice unlocks a new catalytic path and biological function of OsOSC10. The pan‐genome analysis of OSCs within genus Oryza gives insights into the evolutionary trajectory and products diversity of Oryza OSCs.
Prezygotic barriers effectively limit hybridization in a rapid evolutionary radiation
Authors: Uckele Kathryn A. , Vargas Oscar M. , Kay Kathleen M. 
Published: October 2024

Summary
Hybridization is increasingly recognized as an important evolutionary process across the tree of life. In many clades, phylogenomic approaches have permitted unparalleled insight into the extent and frequency of hybridization. However, we continue to lack a deep understanding of the factors that limit and shape patterns of hybridization, especially in evolutionary radiations. In this study, we characterized patterns of introgression across Costus (Costaceae), a young evolutionary radiation of tropical understory plants that maintain widespread interfertility despite exhibiting strong prezygotic reproductive isolation.
We analyzed a phylogenomic dataset of 756 genes from 54 Costus species using multiple complementary approaches – D‐statistics, gene‐tree‐based tests, and phylogenetic network analyses – to detect and characterize introgression events throughout the evolutionary history of the radiation.
Our results identified a moderate number of introgression events, including a particularly ancient, well‐supported event spanning one of the deepest divergences in the clade. Most introgression events occurred between taxa or ancestral lineages that shared the same pollination syndrome (bee‐pollinated or hummingbird‐pollinated).
These findings suggest that prezygotic barriers, including pollinator specialization, have been key to the balance between introgression and reproductive isolation in Costus.
Water Loss From Bagged Leaves During Storage: Why and When?
Authors: Feng Feng , Assouline Shmuel , Rockwell Fulton , Hochberg Uri 
Published: October 2024

ABSTRACTIn ecophysiology leaves are frequently stored for hours after sampling before measuring their leaf water potential (Ψleaf). Here, we address a previously unidentified source of error, that metabolic heat generation can cause continuous water loss from leaves stored in impermeable bags, leading to a Ψleaf drop over time. We tested Ψleaf drop rates under various conditions: two bag materials, two species, initial Ψleaf above or below the turgor loss point (Ψtlp), and storage at 25°C versus 4°C. We partitioned leaf water loss due to condensation on the inner bag surface or permeation through the bag. We found that Ψleaf dropped by up to 0.39 MPa per hour, with 41%–89% of the water leaving the leaf condensed on the inner bag surface. Plastic bags showed higher Ψleaf drop rates than aluminium bags, and leaves above Ψtlp experienced greater drops. Storing leaves at 4°C reduced the Ψleaf drop rate by 60% compared to 25°C. Leaves were 0.2–0.3°C warmer than the bags, likely due to metabolic heating. Our energy balance model suggests that water loss is lower when storing leaves at cooler temperatures, using leaves with low stomatal conductance, deflated bags, and leaves with low Ψleaf.
Photobiomodulation therapy on puncture‐associated pain: A controlled randomized double‐blind clinical trial
Authors: Ferreira Giovanna Fontgalland , Machado Glaucia Gonçales Abud , Roncolato Vinicius Leão , Ramalho Karen Muller , Motta Lara Jansiski , Bussadori Sandra Kalil , Duran Cinthya Cosme Gutierrez , Fernandes Kristianne Porta Santos , Mesquita Ferrari Raquel Agnelli , Chiniforush Nasim , Horliana Anna Carolina Ratto Tempestini 
Published: October 2024

AbstractDental fear and phobia are prevalent worldwide, with local anesthesia being the most feared procedure. This study aimed to determine whether photobiomodulation therapy (PBMT), used as a pre‐anesthetic, could modulate puncture pain and enhance the effectiveness of local anesthesia. In this controlled, randomized, double‐blind study, 49 participants were divided into an experimental group (n = 24), which received infrared laser therapy (100 mW, at 808 nm, 8 J, 80 s at a single point) immediately before standard anesthesia; and control group (n = 25), which received the standard anesthetic technique and sham laser. Pain levels were measured using the visual analog scale, and anesthetic efficacy was assessed through electrical tests (latency), percentage of failures, and cartridge usage. Anxiety levels were evaluated using the Beck Anxiety Inventory. Cardiovascular parameters were evaluated through blood pressure, oxygen levels, and heart rate. This randomized, double‐blind study found no difference between groups in these experimental conditions. The bias toward a positive PBMT result was sufficiently removed. Autonomic responses of the PBMT group were maintained stable during the procedure.
Quality of life and post‐traumatic stress disorder among elderly earthquake survivors: a focus on the February earthquake in Turkey
Authors: Yayla Muhteşem Erol , Gümüştakim Raziye Şule , Ayhan Başer Duygu 
Published: October 2024

ABSTRACTBackgroundOn 6 February, 2023, Turkey experienced a disaster resulting from two major earthquakes. The elderly population stands out as one of the most vulnerable and least adaptable. This study aims to identify the quality of life (QOL) and post‐traumatic stress disorder (PTSD) symptoms among elderly earthquake survivors in Turkey.MethodsThis research was conducted between 1 October, 2023 and 15 December, 2023 as a cross‐sectional design. During the data collection process, survey questions prepared by the researchers (29 questions) and the Elderly Quality of Life Scale (OPQOL‐Bref) and the Impact of Events Scale (IES‐R) were used.ResultsThree hundred and eighty‐four elderly people participated in the study. PTSD was more common in those diagnosed with chronic diseases after the earthquake, in those who had a drug‐free period after the earthquake, in those who smoked and quit smoking, and in those who started psychiatric medication after the earthquake. Post‐traumatic stress was seen more frequently in those with low QOL scores and in those who had a longer period of not using medication after the earthquake. It was observed that QOL was better in married people, high school graduates, those with average income levels, those who did not have chronic diseases before and after the earthquake, those who did not have a drug‐free period after the earthquake, those who were physically active, and those who did not use psychiatric medication after the earthquake.ConclusionChronic disease diagnosis post‐earthquake, drug‐free periods, smoking habits, and psychiatric medication usage were identified as influential factors in PTSD occurrence. Additionally, the study shed light on the profound effect of earthquake‐related experiences, such as house damage, injuries, deaths in first‐degree relatives, displacement, and changes in living conditions, on PTSD prevalence.
At their fingertips: What is the impact of online reporting of domestic violence?
Authors: Fantoni Marylis C. 
Published: October 2024

AbstractGlobal reports indicate that 307 million women have suffered physical or sexual intimate partner violence during the last 12 months. Yet, chronic underreporting of domestic violence (DV) is still a reality in the United States and worldwide. The process of going to a police station and reporting DV is extremely burdensome, leading to numerous psychological effects on the victim and lost opportunity cost of stopping the violence cycle. This study investigates the impacts of bypassing police contact by enabling online reporting and requesting of protective orders (POs) in Brazil. Through novel databases and by using a Synthetic Control, I find that online reporting of DV led to a 29.5% increase in the average monthly requests of POs. This translates to roughly 373 new requests of POs. Implications of these findings are discussed.
Optimal unilateral climate policy with carbon leakage at the extensive and the intensive margins
Authors: Kruse‐Andersen Peter K. , Sørensen Peter Birch 
Published: October 2024

AbstractWe derive the optimal unilateral climate policy for an open economy with a domestic emissions target and aversion to carbon leakage, distinguishing between leakage at the extensive margin, where domestic firms relocate to foreign countries, and leakage at the intensive margin, where domestic firms lose world market shares. Implementing the optimal allocation requires several instruments including a lump‐sum location subsidy to counter leakage at the extensive margin. Simulations indicate substantial leakage at the extensive margin and a social welfare gain of around 0.3 percent of national income from our optimal leakage‐adjusted tax‐subsidy scheme compared to uniform carbon taxation.
Suicide prevention safety planning in the US Department of defense: Qualitative assessment of training
Authors: Norr Aaron M. , Sandel‐Fernandez Devon , Nguyen Janelle , Schacht Reisinger Heather , Reger Greg M. 
Published: October 2024

AbstractIntroductionSuicide rates in the military are a significant public health concern. The suicide prevention safety planning intervention is a brief and effective intervention in which a provider and patient work collaboratively to recognize warning signs of a suicidal crisis and create a defined list of coping strategies and supports for use during future crises (Stanley & Brown, 2012). Implementation of safety planning has been supported by the Department of Defense (DoD), yet readily available training and continuing education for healthcare providers in this intervention is limited and passive in nature. Existing safety planning training experiences and needs of DoD behavioral health providers are unknown. The present study is an exploratory qualitative assessment of current safety planning in DoD to inform the design and development of an interactive virtual standardized training patient.MethodTen military behavioral health providers completed semi‐structured interviews.ResultsThematic content analysis was conducted, and three themes are described in this paper: variety of training received, barriers to receiving suicide safety planning training, and desires for future training. Lack of protected time for training and a lack of access to training resources were identified as key barriers.ConclusionsRich data obtained can help inform the key design features and relevance of new safety planning intervention training approaches
Unravelling the effects of straw return on rice production in central China: Evidence for future policy‐making
Authors: Shen Xue , Jin Shan , Liang Zhihui , Ao Rongjun 
Published: October 2024

AbstractThe practice of straw return represents an effective and sustainable approach to recycling and reusing agricultural residues, thereby significantly contributing to the circular economy within the agri‐food sector. Although this practice holds significant promise for enhancing soil quality and mitigating pollution resulting from straw burning, the realization of these substantial benefits depends on the extent to which farmers are willing to embrace this practice, a decision closely tied to the anticipated economic benefits it offers. The present study aimed to explore the effect of straw return on rice production in terms of rice yield and net return using a representative household survey of 1752 rice farmers in central China. The results showed that there exist threshold effects of the years of straw return on both rice yield and net income, which decline as years of straw return increase below the thresholds, and then increase linearly above the thresholds. Overall, after implementing the straw return practice, it took an average of about 3.5 years to observe a positive effect on rice yields and about 7.5 years to see an increase in net income. The estimates were robust to threshold regression, Lasso regression and random forest regression methods. Nonetheless, the provision of essential technical guidance to farmers via agricultural cooperatives could contribute to expediting the realization of the benefits associated with implementing straw return practices. The results imply that the current incentive mechanism aimed at diffusing straw return practice should be fine‐tuned by considering the time required for straw return to generate economic benefits in specific contexts, ensuring better compensation for initial losses. Interventions that shorten the time needed to realize these benefits, such as providing additional technical training for farmers, should be implemented. Furthermore, the uncertainty regarding the time frame for benefit realization should be communicated clearly and effectively to farmers.
Teachers’ Gestures and How They Matter
Authors: Church R. Breckinridge , Perry Michelle , Singer Melissa A. , Cook Susan Wagner , Alibali Martha Wagner 
Published: October 2024

AbstractHow do teachers’ gestures influence students’ learning? This article reviews research investigating the role of gestures in communication, focusing on teachers’ communication with their students, primarily in mathematics and science instruction. We first briefly consider gesture's role in communication more generally as a backdrop for considering teaching as a special context for communication. We then describe teachers’ spontaneous gesturing in teaching contexts, and we consider how teachers’ spontaneous gestures might influence students’ learning. We then consider experimental studies that provide causal support for the effects of teachers’ gestures on students’ learning. We conclude by discussing future directions and implications for educational practice.
Serum total antioxidant status in dogs: Reference intervals and influence of multiple biological and analytical factors
Authors: Perez‐Montero B. , Fermin‐Rodriguez M. L. , Portero‐Fuentes M. , Sarquis J. , Caceres S. , del Portal J. C. Illera , de Juan L. , Miro G. , Cruz‐Lopez F. 
Published: October 2024

AbstractBackgroundTotal antioxidant status (TAS) is one of the most widely used oxidative stress biomarkers, but the lack of canine RI and the influence of analytical factors hinder its application in clinical practice.ObjectivesThe aims of this study were to establish canine assay‐specific RI for TAS and evaluate the sources of biological variation and the association between TAS and multiple hematologic and biochemical variables.MethodsBlood samples from 190 clinically healthy dogs were collected, encompassing pet dogs (82), police dogs (56), and shelter dogs (52). After hematologic and biochemical analysis, serum TAS was determined by means of a commercial 2,2′‐azinobis (3‐ethylbenzthiazolin‐6‐sulfonic acid) (ABTS) test. The American Society for Veterinary Clinical Pathology guidelines were followed to establish the RI, employing nonparametric methods. Univariate analysis and multivariate analysis were conducted to assess the influence of biological and analytical variables, yielding a final regression model.ResultsThe final reference population comprised 143 dogs, for which the RI was established (1.41–2.27 mmol/L). Partitioning was applied to the three study groups. The regression model revealed that police dogs had significantly higher TAS values than pet dogs. Furthermore, significant associations between four biochemical variables (albumin, globulins, cholesterol, and aspartate aminotransferase) and serum TAS were found.ConclusionsThis is the first study to establish RI for serum TAS in a large and heterogeneous canine population and provide data on its relationship with analytical variables. These findings could potentially improve the interpretation of TAS in clinical environments.
Equivalence in intradermal reactions to histamine and compound 48/80 in dogs before and after sedation with dexmedetomidine or a 1:20 combination of medetomidine and vatinoxan
Authors: Santoro Domenico , Moura Raiane A. , McKenzie Stuart R. , Chiavaccini Ludovica 
Published: October 2024

AbstractBackgroundIntradermal allergen testing (IDAT) is commonly used to formulate allergen‐specific immunotherapy, a pillar treatment for canine atopic dermatitis. Many sedatives have shown histaminergic or anti‐histaminergic effects and thus been deemed unsuitable for IDAT.ObjectiveThe goal of this study was to determine whether, in healthy dogs, dexmedetomidine (Dexdomitor) or a 1:20 combination of medetomidine and vatinoxan (Zenalpha) will affect intradermal reactions compared to unsedated dogs.AnimalsTen privately owned healthy dogs were enrolled in this equivalence study.Materials and MethodsWheal formation was subjectively and objectively assessed in conscious then sedated dogs. Dogs were randomly sedated with either Dexdomitor (dexmedetomidine [0.5 mg/m2]) or Zenalpha (medetomidine [1 mg/m2/vatinoxan] 20 mg/m2) intramuscularly. Once sedated, five 10‐fold histamine (100–0.01 μg/mL) and compound 48/80 (200–0.02 μg/mL) dilutions were intradermally injected into the lateral thorax. The study was repeated on the opposite side with the alternative sedation 1 week later. Quality of sedation, cardiorespiratory function and rectal temperature were recorded every 5 min.ResultsThere was no difference in the median values of the reactions with either sedative when compared to unsedated dogs. Dexdomitor and Zenalpha achieved an equivalence in both subjective and objective scoring systems for all concentrations tested. A faster median time to sedation (10 vs. 18 min, p = 0.013) was seen with Zenalpha compared to Dexdomitor. Although both sedatives depressed the cardiovascular function, such parameters were less affected by Zenalpha than by Dexdomitor (p ≤ 0.001).Conclusions and Clinical RelevanceOwing to the lack of effects on wheal formation, both sedatives are appropriate for sedating dogs undergoing IDAT. Although, such results should be validated in allergic dogs. Zenalpha may induce more rapid and reliable sedation than Dexdomitor.
Community screening for iron deficiency in reproductive aged women: Lessons learnt from Australia
Authors: MacLean Beth , Lim Jayne , Fuller Jess , Wylie Riki , Joo Judie Yeleen , Al‐Sharea Annas , Cheyyur Jaahnavi , Ng Henry , Zhang Sijing , Ahmed Mubashshira , Dugan Cory , Richards Toby 
Published: October 2024

AbstractBackground and ObjectivesReproductive‐aged women are at an increased risk of developing iron deficiency (ID). We aimed to develop a non‐invasive screening tool to identify ID in women and assess the acceptability of screening.Study Design and MethodsWe screened women (age 18–49 years) in the community of Western Australia. Primary outcome: acceptability of screening, assessed by the feasibility of recruiting the required sample size (n = 323). Secondary outcomes: Hand grip strength, finger prick haemoglobin concentration (Hb), prevalence of heavy menstrual bleeding (HMB), diet, pregnancy history, blood donation, symptoms of ID and history of ID or anaemia (Hb < 120 g/L). Those with Hb <130 g/L and no history of iron therapy in the past 2 years were given referrals for venous full blood count and ferritin sampling.ResultsAcross 5 days, we recruited 640 eligible women. Of which, 178 (28%) had HMB and 79 (12%) were anaemic. Mean age was 33.5 ± 9.2 years, and mean Hb was 132.4 ± 11.9 g/L. In the past 2 years: 335 (52%) were diagnosed with ID or anaemia; 322 (50%) had taken oral iron; and 210 (33%) had an intravenous iron infusion. Vegetarian diets were followed by 89 (14%); 40 (6%) were regular blood donors; 290 (45%) had a previous pregnancy.HMB increased the risk of symptoms of ID and having prior ID/anaemia diagnosis (67% vs. 47%) or treatment (p < 0.022). Hand grip strength showed a positive relationship with both Hb (adjusted R2 = 0.012, p = 0.004) and ferritin (adjusted R2 = 0.135, p = 0.005).ConclusionID screening was well accepted by women in the community, with high recruitment rates over a short period. Future screening tool development may consider incorporating hand grip strength and HMB assessment.
An In Vitro Platform for Pharmacokinetic Quantification and Optimization of Cerebrospinal Fluid Filtration and Drug Circulation
Authors: Sass Lucas R. , Khani Mohammadreza , Giordano Michael C. , McCabe Aaron R. , Lad Shivanand P. , Martin Bryn A. 
Published: October 2024

Abstract
Modification of cerebrospinal fluid (CSF) transport dynamics is an expanding method for treating central nervous system injury and diseases. One application of this route is to modify the distribution of solutes in the CSF; however, few tools currently exist for this purpose. The present study describes the use of a subject-specific in vitro CSF phantom to perform a parametric evaluation of the Neurapheresis™ CSF Management System (NP) for both CSF filtration and intrathecal drug circulation. An in vitro CSF phantom was constructed which included realistic anatomy for the complete subarachnoid space (SAS). This platform was configured to test multiple parametric modifications of a dual-lumen catheter and filtration system. Calibrated mapping of tracer distribution and area under the curve (AUC) measurements were used to compare filtration and intrathecal-circulation schemes using the NP device versus the clinical standards of care. The NP device showed potential advantages over lumbar drain (LD) for clearance of simulated subarachnoid hemorrhage (SAH), especially in the spinal canal. Use of the NP device in combination with simulated intracerebroventricular (ICV) drug infusion resulted in an increased extent and uniformity of tracer spread compared to ICV alone. NP improved clearance of simulated subarachnoid hemorrhage compared to LD and increased uniformity of tracer concentration via simulated ICV, providing support for NP use in these scenarios. The in vitro CSF phantom system presented here quantitatively described the effects of parametric boundary modification on solute distribution in the intrathecal space.
Complete genome sequence of
Sphingobacterium thalpophilum
BAA-1094
Authors: Adaikpoh Barbara I. , Eustáquio Alessandra S. 
Published: October 2024

ABSTRACT

We report the complete genome of
Sphingobacterium thalpophilum
BAA-1094 obtained from the American Type Culture Collection. The genome has one circular chromosome (5.7 Mbp). The rather low average nucleotide identity of 76.5% to the type strain
Sphingobacterium thalpophilum
DSM 11723 suggests the need for reclassification.
Completed genome segments of Maciel, Lechiguanas, and Laguna Negra orthohantaviruses
Authors: Shedroff Elizabeth , Whitmer Shannon L. M. , Mobley Melissa , Morales-Betoulle Maria , Martin Maria Laura , Brignone Julia , Sen Carina , Nazar Yael , Montgomery Joel M. , Klena John D. 
Published: October 2024

ABSTRACT

New World orthohantaviruses are rodent-borne tri-segmented viruses that cause hantavirus cardiopulmonary syndrome in humans in the Americas. Molecular diagnostics for orthohantaviruses can be improved with more sequence data. Reported here are completed genomes for Lechiguanas, Maciel, and Laguna Negra viruses.