bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium simiumcausing human malaria: a zoonosis with outbreak potential in the Rio de Janeiro Brazilian Atlantic forest
Authors: Brasil Patrícia , Zalis Mariano Gustavo , de Pina-Costa Anielle , Siqueira Andre Machado , Júnior Cesare Bianco , Silva Sidnei , Lisboa Areas André Luiz , Pelajo-Machado Marcelo , de Alvarenga Denise Anete Madureira , da Silva Santelli Ana Carolina Faria , Albuquerque Hermano Gomes , Cravo Pedro , de Abreu Filipe Vieira Santos , Peterka Cassio Leonel , Zanini Graziela Maria , Suárez Mutis Martha Cecilia , Pissinatti Alcides , Lourenço-de-Oliveira Ricardo , de Brito Cristiana Ferreira Alves , Ferreira-da-Cruz Maria de Fátima , Culleton Richard , Daniel-Ribeiro Cláudio Tadeu 
Published: March 2017

SummaryBackgroundMalaria was eliminated from Southern and Southeastern Brazil over 50 years ago. However, an increasing number of autochthonous episodes attributed toPlasmodium vivaxhave been recently reported in the Atlantic forest region of Rio de Janeiro State.As P. vivax-like non-human primate malaria parasite speciesPlasmodium simiumis locally enzootic, we performed a molecular epidemiological investigation in order to determine whether zoonotic malaria transmission is occurring.MethodsBlood samples of humans presenting signs and/or symptoms suggestive of malaria as well as from local howler-monkeys were examined by microscopy and PCR. Additionally, a molecular assay based on sequencing of the parasite mitochondrial genome was developed to distinguish betweenP. vivaxandP. simium, and applied to 33 cases from outbreaks occurred in 2015 and 2016.ResultsOf 28 samples for which the assay was successfully performed, all were shown to beP. simium, indicating the zoonotic transmission of this species to humans in this region. Sequencing of the whole mitochondrial genome of three of these cases showed thatP. simiumis most closely related toP. vivaxparasites from South American.FindingsThe explored malaria outbreaks were caused byP. simium, previously considered a monkey-specific malaria parasite, related to but distinct fromP. vivax, and which has never conclusively been shown to infect humans before.InterpretationThis unequivocal demonstration of zoonotic transmission, 50 years after the only previous report ofP. simiumin man, leads to the possibility that this parasite has always infected humans in this region, but that it has been consistently misdiagnosed asP. vivaxdue to a lack of molecular typing techniques. Thorough screening of the local non-human primate and anophelines is required to evaluate the extent of this newly recognized zoonotic threat to public health and malaria eradication in Brazil.FundingFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado de Rio de Janeiro (Faperj), The Brazilian National Council for Scientific and Technological Development (CNPq), JSPS Grant-in-Aid for scientific research, Secretary for Health Surveillance (SVS) of the Ministry of Health, Global Fund, and PRONEX Program of the CNPq.
Validating the relationship between trust, commitment, economic and non-economic satisfaction in South African buyer-supplier relationships
Authors: Mpinganjira Mercy , Roberts-Lombard Mornay , Svensson Göran 
Published: March 2017

Purpose
The paper aims to validate the relationship between satisfaction, trust and commitment in South African business buyer–supplier relationships. Satisfaction was looked at from the dual perspective of economic and non-economic satisfaction.

Design/methodology/approach
A research model showing hypothesised relationships between the constructs was tested using data collected from 250 large companies in South Africa. Structural equation modelling using AMOS software was used to analyse the data. The results provide support for the distinctiveness of both economic and non-economic satisfaction and the mediating effect of trust.

Findings
The findings show that economic satisfaction exerts a direct influence on trust, which, in turn, has a direct influence on commitment. Trust and commitment were both found to have a positive influence on non-economic satisfaction.

Research limitations/implications
The tested nomological network of business relationship quality dimensions validates some of the recent findings by Ferro et al. (2016) in the South African context of buyer–supplier relationships.

Originality/value
The study contributes to a better understanding of satisfaction and its relationship with other relationship quality constructs, especially in the context of large companies in South Africa.
Significant decrease in local butterfly community during the last 15 years in a calcareous hill of the middle Korea
Authors: Choi Sei‐Woong , Kim Sung‐Soo , Kwon Tae‐Sung , Park Haechul 
Published: March 2017

AbstractRecently the significant decreases of species richness and abundance among terrestrial animals including butterflies are reported due to habitat change, overexploitation, and global warming. We compared the butterfly species composition and abundance from 1999 and 2014–2015 in a calcareous hill site of the middle part of Korea using a line transect method. There was a significant decrease in the number of individuals (abundance) and the number of species (richness) from 1999 to 2014–2015. This decrease was more prevalent among northern species than southern species, and the local extinct species were more prevalent among northern species, showing the influence of global warming on butterfly assemblages. However, no impact of habitat change was observed because of maintenance of the grasslands, which is caused by the dry soils of the calcareous region.
Epidemiology and clinical characteristics of patients hospitalized for ocular trauma in South‐Central China
Authors: Wang Wanpeng , Zhou Yalan , Zeng Jun , Shi Meng , Chen Baihua 
Published: March 2017

AbstractPurposeOcular trauma is a major cause of visual loss, but little is known about its epidemiology and clinical characteristics in China. The aim of this study was to determine the prevalence and clinical characteristics of ocular trauma and assess prognostic factors in Changsha, Hunan, located in South‐Central China.MethodsA retrospective case series (ICD codes: S05) study of ocular trauma in patients was performed at the Second Xiangya Hospital, Central South University, from 1 January 2010 to 31 December 2014. Demographic information, injury causes, ocular trauma types and initial and final visual acuity (VA) were recorded and analysed. The ocular trauma score (OTS) was calculated to assess the extent of the eye injury, prognosis and factors associated with visual impairment. All patient data were collected from the medical records system.ResultsOf the 2009 patients presenting during this 5‐year period, 1695 (84.4%) were males and 314 (15.6%) were females. The average age of all patients was 37.0 ± 19.3 years (range from 1 to 87 years). The age distribution showed a peak in the ocular trauma population in the 41‐ to 50‐year age group (24%, n = 482), followed by the 51‐ to 60‐year age group (16.9%, n = 339). Overall, open‐globe injuries had a higher frequency (70.7%, n = 1420) than closed‐globe injuries (28.6%, n = 575) and thermal/chemical injuries (0.7%, n = 14). Of the open‐globe injuries, corneal penetration was the most common injury (32.2%, n = 646) followed by rupture (21.5%, n = 432) and an intraocular foreign body (16.2%, n = 325). Overall, the most frequent ocular trauma setting was the workplace (39.6%, n = 795), followed by the home (28.4%, n = 570), and the most frequent activity was ironwork. Firecracker‐ and firework‐associated ocular trauma was significantly higher during the months of January and February than during other months (50.0%, n = 112, p < 0.001). In patients under 18 years, the most frequently occurring injury was open globe (84.5%, n = 349) with corneal penetration (59.3%, n = 245). Overall, an operation was performed in 1865 (92.8%) cases, and 144 cases (7.2%) were treated conservatively with medication. The average OTS score was 47.1 ± 19.5. The visual prognosis was closely correlated with the initial VA (r = 0.570, p = 0.000) and the OTS score (r = 0.603, p = 0.000).ConclusionThis study describes the epidemiology and clinical characteristics of patients hospitalized for ocular trauma in South‐Central China. Notably, a high rate of firework‐related ocular trauma occurred during the months adjacent to the Chinese New Year festival. Specific injury prevention strategies, such as the use of protective eyewear, need to target the workplace to reduce the incidence and severity of ocular trauma.
Glistenings, anterior/posterior capsular opacification and incidence of Nd:YAGlaser treatments with two aspheric hydrophobic acrylic intraocular lenses – a long‐term intra‐individual study
Authors: Johansson Björn 
Published: March 2017

AbstractPurposeTo compare two hydrophobic acrylic intraocular lenses (IOLs) regarding long‐term anterior/posterior capsular opacification (ACO/PCO) development and need for neodymium:Yttrium‐Aluminum‐Garnet (Nd:YAG) laser treatment due to visually disturbingPCO, and to study development of glistenings in theIOLmaterials.MethodsIn a prospective, randomized, intra‐individual, comparative trial, 50 cataract patients received either an AcrySofIQ®SN60WF(Alcon, Fort Worth, TX, USA) or a Tecnis®ZCB00 (Abbott Medical Optics, Santa Ana, CA, USA)IOLin the first operated eye, and the second eye received theIOLtype not implanted in the first eye. Anterior/posterior capsular opacification (ACO/PCO) and fibrosis were monitored with slit‐lamp photography and semi‐automated digital analysis 2 and 3 years postoperatively. Glistenings were semi‐quantitatively assessed in slit‐lamp photographs. Nd:YAGlaser treatment for visually disturbingPCOwas monitored.ResultsVisual outcomes were similar for the twoIOLs. Anterior capsular fibrosis and/or opacification developed more often inSN60WFeyes. MeanPCOarea percentage was larger inZCB00 eyes 3 years after surgery, but severity score did not differ with statistical significance between the twoIOLs. SixZCB00 eyes and 2SN60WFeyes underwent Nd:YAGlaser treatment during a mean of 4 years 8 months after surgery. This difference was not statistically significant. A high amount of glistenings developed in mostSN60WF IOLs, while only fewZCB00IOLs displayed a low degree of glistenings.ConclusionVisual outcomes,PCOdevelopment over time and need for Nd:YAGlaser treatment were similar for the twoIOLs. Anterior capsule fibrosis/contraction and glistenings were more pronounced with theSN60WF IOL.
Osmodehydration assisted by ohmic heating/pulse vacuum in apples (cv. Fuji): retention of polyphenols during refrigerated storage
Authors: Moreno Jorge , Zúñiga Pamela , Dorvil Franck , Petzold Guillermo , Mella Karla , Bugueño Graciela 
Published: March 2017

SummaryApples are a widely consumed fruit and have a high polyphenol content. The aim of this study was to analyse the combined effects of osmotic dehydration (OD) and ohmic heating (OH) with a pulsed vacuum (PV) on polyphenol retention during the stored refrigeration of apple cubes. Treatments were performed using a 65°Brix sucrose solution at 30, 40 or 50 °C for 120 min, and then, samples were stored for 28 days at 5 °C. OH provides an electric field of 13 V cm−1, and a pulsed vacuum was applied for 5 min at the beginning of the process. The results indicated that a lower temperature process (30–40 °C) resulted in the retention of more polyphenol compounds after treatment than a higher temperature process (50 °C). Nevertheless, during refrigerated storage, we observed that 50 °C preserved these compounds better due to polyphenol oxidase inactivation. PVOD/OH treatment at 50 °C was determined to be the best retention of polyphenols from the fresh apple for dehydrating apples.
Effect of pulse flours on the physiochemical characteristics and sensory acceptance of baked crackers
Authors: Millar Kim Adrienne , Barry‐Ryan Catherine , Burke Róisín , Hussey Karen , McCarthy Sinéad , Gallagher Eimear 
Published: March 2017

SummaryPulse flours offer nutritional alternatives to wheat flour in the production of baked snacks due to their high protein and fibre levels and low glycaemic index. In this study, broad‐bean (Vicia faba), yellow‐pea and green‐pea (Pisum sativum) flours were each blended with wheat flour at 40% in the formulation of chemically leavened crackers. The effects of flour type and baking time on the physiochemical properties, sensory acceptability, nutritional composition and antioxidant activity of the crackers were observed in comparison with 100% wheat crackers. Broad‐bean crackers had the highest protein content and antioxidant activity (13 g per 100 g DM and 38.8 mgAAE per 100 g DM, respectively). Yellow‐pea crackers had the highest fibre content (12 g per 100 g DM). Physical dimensions and colour attributes were significantly affected by pulse‐flour substitution. Yellow‐pea and broad‐bean crackers were significantly preferred by consumers compared to the control, demonstrating the potential application of these flours to improve the eating quality and nutritional profile of crackers.
Improving the quality of meat‐free sausages using κ‐carrageenan, konjac mannan and xanthan gum
Authors: Majzoobi Mahsa , Talebanfar Samaneh , Eskandari Mohammad Hadi , Farahnaky Asgar 
Published: March 2017

SummaryHigh consumption of processed red meat can cause health issues. Therefore, production of high‐quality meat‐free food alternatives is necessary. The main objective of this study was to use hydrocolloids including κ‐carrageenan, konjac mannan and xanthan gum at 0, 0.3, 0.6, 1.0 and 1.5% w/w to improve the quality of meat‐free sausages formulated by soy protein isolated, texturised soy protein, corn starch, vegetable oil and spices. With the addition of the tested hydrocolloids, the lightness of the sausages improved. κ‐Carrageenan and konjac mannan significantly improved the water‐holding capacity and texture and reduced the cooking loss of the samples, while xanthan gum showed no considerable effect on these parameters. Konjac mannan and κ‐carrageenan (up to 0.6%) improved the general acceptability of the sausages, while inclusion of xanthan gum made no significant difference. Unlike xanthan gum, κ‐carrageenan followed by konjac mannan was highly successful in improving the overall quality of meat‐free sausages.
Glazing herring (Clupea harengus) fillets with herring muscle press juice: effect on lipid oxidation development during frozen storage
Authors: Cavonius Lillie R. , Undeland Ingrid 
Published: March 2017

SummaryIn this study, fish muscle press juice, PJ, was evaluated as an antioxidative liquid for glazing of fish fillets prior to frozen storage. PJ has in previous studies efficiently protected fish mince lipids against haemoglobin‐mediated oxidation. Frozen herring fillet were glazed with water, PJ or 50 mm phosphate buffer of the same pH as herring PJ (6.5). Over 52 weeks, peroxide value (PV), TBA‐reactive substances (TBARS), rancid odour and colour were monitored. From 26 to 52 weeks, water‐glazed fillets were significantly (P < 0.05) more oxidised compared to fillets glazed with PJ or buffer, most clearly shown by PV and TBARS data. The protective effects of both PJ and buffer compared to water could indicate a role, for example, of the physiological ionic strength. The finding that PV became elevated in PJ which was frozen stored alone in a thin layer indicates that PJ stripped from, for example, trace lipids and haemoglobin would provide better protection.
Comparison of direct electrocaloric characterization methods exemplified by 0.92 Pb(Mg1/3Nb2/3)O3‐0.08 PbTiO3 multilayer ceramics
Authors: Molin Christian , Peräntie Jani , Le Goupil Florian , Weyland Florian , Sanlialp Mehmet , Stingelin Natalie , Novak Nikola , Lupascu Doru C. , Gebhardt Sylvia 
Published: March 2017

AbstractElectrocaloric device structures have been developed as multilayer ceramics (MLCs) based on fundamental research carried out on PMN‐8PT bulk ceramics. Two different MLC structures were prepared with nine layers each and layer thicknesses of 86 μm and 39 μm. The influence of the device design on its properties has been characterized by microstructural, dielectric, ferroelectric, and direct electrocaloric measurement. For direct characterization two different methods, ie temperature reading (thermistor and thermocouple) and heat flow measurement (differential scanning calorimetry), were used. A comparison of results revealed a highly satisfactory agreement between the different methods. This study confirms that MLCs are promising candidates for implementation into energy‐efficient electrocaloric cooling systems providing large refrigerant volume and high electrocaloric effect. Due to their micron‐sized active layers, they allow for the application of high electric fields under low operation voltages. We measured a maximum electrocaloric temperature change of ΔT=2.67 K under application/withdrawal of an electric field of ΔE=16 kV mm−1, which corresponds to operation voltages below 1.5 kV.
Bacterial and Fungal Endophthalmitis
Authors: Durand Marlene L. 
Published: March 2017

SUMMARY
Endophthalmitis is a severe eye infection that may result in permanent loss of useful vision in the affected eye. Most cases are exogenous and occur as a complication of cataract surgery, an intravitreal injection, or penetrating ocular trauma. Endogenous endophthalmitis results from hematogenous seeding of the eye by bacteria or fungi, but bacteremia or fungemia may be transient and patients may present without symptoms of systemic infection. Nearly all endophthalmitis patients present with decreased vision, and some also have eye pain. Eye examination usually reveals a hypopyon and intraocular inflammation. Diagnosis is clinical, supported by cultures of the vitreous and/or aqueous or by blood cultures in some endogenous cases. Molecular diagnostic techniques have been used in research laboratories for pathogen identification in endophthalmitis and offer the possibility of rapid diagnosis, including in culture-negative cases. Intravitreal injection of antibiotics is the most important component of treatment; some cases also benefit from surgical debridement of the vitreous by a vitrectomy. The visual outcome depends partly on the pathogen: coagulase-negative staphylococcal endophthalmitis has a better prognosis than does streptococcal endophthalmitis, for example. Endophthalmitis is a medical emergency, and prompt diagnosis and treatment are essential for saving vision.
Prospective Evaluation of Light Scatter Technology Paired with Matrix-Assisted Laser Desorption Ionization
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Time of Flight Mass Spectrometry for Rapid Diagnosis of Urinary Tract Infections
Authors: Montgomery Sandra , Roman Kiana , Ngyuen Lan , Cardenas Ana Maria , Knox James , Tomaras Andrew P. , Graf Erin H. 
Published: March 2017

ABSTRACT
Urinary tract infections are one of the most common reasons for health care visits. Diagnosis and optimal treatment often require a urine culture, which takes an average of 1.5 to 2 days from urine collection to results, delaying optimal therapy. Faster, but accurate, alternatives are needed. Light scatter technology has been proposed for several years as a rapid screening tool, whereby negative specimens are excluded from culture. A commercially available light scatter device, BacterioScan 216Dx (BacterioScan, Inc.), has recently been advertised for this application. Paired use of mass spectrometry (MS) for bacterial identification and automated-system-based susceptibility testing straight from the light scatter suspension might provide dramatic improvement in times to a result. The present study prospectively evaluated the BacterioScan device, with culture as the reference standard. Positive light scatter specimens were used for downstream rapid matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS organism identification and automated-system-based antimicrobial susceptibility testing. Prospective evaluation of 439 urine samples showed a sensitivity of 96.5%, a specificity of 71.4%, and positive and negative predictive values of 45.1% and 98.8%, respectively. MALDI-TOF MS analysis of the suspension after density-based selection yielded a sensitivity of 72.1% and a specificity of 96.9%. Antimicrobial susceptibility testing of the samples identified by MALDI-TOF MS produced an overall categorical agreement of 99.2%. Given the high sensitivity and negative predictive value of results obtained, BacterioScan 216Dx is a reasonable approach for urine screening and might produce negative results in as few as 3 h, with no downstream workup. Paired rapid identification and susceptibility testing might be useful when MALDI-TOF MS results in an organism identification, and it might decrease the time to a result by more than 24 h.
pH Optimum of Hemagglutinin-Mediated Membrane Fusion Determines Sensitivity of Influenza A Viruses to the Interferon-Induced Antiviral State and IFITMs
Authors: Gerlach Thomas , Hensen Luca , Matrosovich Tatyana , Bergmann Janina , Winkler Michael , Peteranderl Christin , Klenk Hans-Dieter , Weber Friedemann , Herold Susanne , Pöhlmann Stefan , Matrosovich Mikhail 
Published: March 2017

ABSTRACT
The replication and pathogenicity of influenza A viruses (IAVs) critically depend on their ability to tolerate the antiviral interferon (IFN) response. To determine a potential role for the IAV hemagglutinin (HA) in viral sensitivity to IFN, we studied the restriction of IAV infection in IFN-β-treated human epithelial cells by using 2:6 recombinant IAVs that shared six gene segments of A/Puerto Rico/8/1934 virus (PR8) and contained HAs and neuraminidases of representative avian, human, and zoonotic H5N1 and H7N9 viruses. In A549 and Calu-3 cells, viruses displaying a higher pH optimum of HA-mediated membrane fusion, H5N1-PR8 and H7N9-PR8, were less sensitive to the IFN-induced antiviral state than their counterparts with HAs from duck and human viruses, which fused at a lower pH. The association between a high pH optimum of fusion and reduced IFN sensitivity was confirmed by using HA point mutants of A/Hong Kong/1/1968-PR8 that differed solely by their fusion properties. Furthermore, similar effects of the viral fusion pH on IFN sensitivity were observed in experiments with (i) primary human type II alveolar epithelial cells and differentiated cultures of human airway epithelial cells, (ii) nonrecombinant zoonotic and pandemic IAVs, and (iii) preparations of IFN-α and IFN-λ1. A higher pH of membrane fusion and reduced sensitivity to IFN correlated with lower restriction of the viruses in MDCK cells stably expressing the IFN-inducible transmembrane proteins IFITM2 and IFITM3, which are known to inhibit viral fusion. Our results reveal that the pH optimum of HA-driven membrane fusion of IAVs is a determinant of their sensitivity to IFN and IFITM proteins.

IMPORTANCE
The IFN system constitutes an important innate defense against viral infection. Substantial information is available on how IAVs avoid detection by sensors of the IFN system and disable IFN signaling pathways. Much less is known about the ability of IAVs to tolerate the antiviral activity of IFN-induced cellular proteins. The IFN-induced proteins of the IFITM family block IAV entry into target cells and can restrict viral spread and pathogenicity. Here we show for the first time that the sensitivity of IAVs to the IFN-induced antiviral state and IFITM2 and IFITM3 proteins depends on the pH value at which the viral HA undergoes a conformational transition and mediates membrane fusion. Our data imply that the high pH optimum of membrane fusion typical of zoonotic IAVs of gallinaceous poultry, such as H5N1 and H7N9, may contribute to their enhanced virulence in humans.