bluuebunny/crossref_metadata_2025_split huggingface dataset:
Embryonic heat conditioning increases lipolytic gene expression in broiler chicks at day 4 post-hatch
Authors: Sulaiman Usman , Vaughan Reagan S. , Siegel Paul , Liu Dongmin , Gilbert Elizabeth Ruth , Cline Mark Andrew 
Published: September 2024

IntroductionExposure to elevated temperatures during incubation is known to induce epigenetic changes that are associated with immunological and stress-response differences at a later age. Reports on its effects on the adipose tissue are still scarce. In this experiment, we investigated the effect of embryonic heat conditioning (EHC) on growth, adipose tissue mRNA and global DNA methylation in broiler chicks at day 4 post-hatch.MethodsFertile eggs were divided into two groups: control and EHC. Eggs in the control group were incubated at 37.8°C and 80% relative humidity from day 0 to day 18.5 (E0 to E18.5). The EHC eggs were subjected to an intermittent increase in temperature to 39.5°C and 80% relative humidity from E7 to E16 for 12 h (07:30–19:30) per day. On day 4 post-hatch, control and EHC chicks were subjected to 36°C using three time points: 0 (no heat challenge serving as the control), and 2 and 12 h relative to start of the heat challenge. Fifteen chicks were sampled from each group for every timepoint. Body weight was recorded before euthanasia and subcutaneous adipose tissue was collected.ResultsBody weights were similar in control and EHC groups. Diacylglycerol O-acyltransferase 2 (DGAT2) mRNA was lower in the EHC group at time 0 relative to control. Hormone-sensitive lipase (HSL) mRNA was greater in the EHC than control group at the 0 h timepoint. Heat challenge affected adipose tissue DNA methylation, with methylation highest at 12 h into the heat challenge.DiscussionThese findings highlight the dynamic molecular responses of chicks to heat stress during early post-hatch development and suggest that EHC may affect heat stress responses and adipose tissue development through mechanisms involving lipid remodeling and DNA methylation.
Exploring the correlates and nature of subjective anomalous interactions with objects (psychometry): a mixed methods survey
Authors: Simmonds-Moore Christine A. 
Published: September 2024

IntroductionPsychometry refers to the experience of receiving information about a person or thing by contact with a given object. There is little research to date on the psychological correlates of psychometry and no systematic qualitative research on the nature of the experience itself.MethodA convergent mixed methods online survey sought to explore how synesthesia and autonomous sensory meridian response (ASMR) correlate with a range of anomalous experiences, including psychometry, among members of the public. Those who reported that they had experienced psychometry were invited to describe their experiences in an open ended section.ResultsResults indicate that those who experience psychometry scored higher on a measure of ASMR than those who did not. Those who experience synesthesia also scored significantly higher on a measure of ASMR than those who did not. However, synesthesia was not significantly associated with psychometry. Both ASMR and synesthesia were associated with tendencies to report anomalous experiences (with and without a paranormal attribution). A thematic analysis found five themes including: a flash of imagery; lived feelings and intense emotions; noesis and perspective taking/empathy. Subjective psychometry experiences seem to reflect emotional information that is experienced as different to one’s normal experiences and felt to be from the perspective of another person.DiscussionResults are discussed and quantitative and qualitative findings are integrated.
The relationship between risk perceptions and negative emotions in the COVID-19: a meta-analysis
Authors: Zhou Ruihua , Shi Kan , Song Xudong , Li Shuqi , Zhou Wei 
Published: September 2024

BackgroundCOVID-19, as a significant public health issue, has had a major impact on the mental health of people worldwide. Research shows a significant positive correlation between individuals’ risk perception levels and negative emotions during the outbreak of COVID-19. However, some studies also suggest that the relationship between the two is not significant. Therefore, we will conduct a meta-analysis to explore the relationship between risk perception and negative emotions from cultural, temporal, and individual psychological perspectives.MethodsSearches were conducted in the Web of Science, Pub Med, Google Scholar, PsycINFO, Scopus, and China National Knowledge Infrastructure databases, focusing on publications from January 2020 onwards, specifically targeting studies examining the relationship between risk perception and negative emotion during COVID-19.ResultsA total of 58 papers with 85 effect sizes were meta-analyzed using Comprehensive Meta-Analysis 3.0 software, with a combined sample of 83,948 individuals. Risk perception of COVID-19 showed a moderate positive correlation with negative emotions (r = 0.211, 95%CI [0.18, 0.24]). There was no moderating effect of tight-loose cultures on the relationship between risk perception of COVID-19 and negative emotions. However, the epidemic period, gender ratio, and measurement methods did have moderating effects on the relationship between risk perception of COVID-19 and negative emotions.ConclusionIn future research, we can further develop theories related to the risk perception of COVID-19 and negative emotions, and based on these, formulate interventions to promote people’s mental health.
The use of intraoperative CT-neuronavigation in Wiltse approach. A technical note
Authors: Battistelli Marco , Valeri Federico , D’Ercole Manuela , Izzo Alessandro , Rapisarda Alessandro , Polli Filippo Maria , Montano Nicola 
Published: September 2024

IntroductionThe paraspinal approach was first introduced in 1968 and later refined by Leon Wiltse to gain access to the lateral interevertebral foraminal region. However, challenges can arise due to unfamiliarity with this approach, unique patient anatomy, or in case of revision surgery, potentially elevating the risk of complications and/or poor outcome.MethodsHere we report on two cases in which the intraoperative Oarm CT neuronavigation was used during a Wiltse approach. Under general anesthesia, the spinous process near the surgical level is exposed through a midline incision. The patient's reference anchor is then attached to the exposed spinous process. Intraoperative CT is acquired and transferred to the Stealth Station S8 Surgical Navigation System (Medtronic). The Wiltse approach is now performed through a paramedian incision under neuronavigation guidance and perfectly tailored to the patient's unique anatomy.ResultsThe first case was a patient harboring a left lumbar intraextraforaminal schwannoma and the second one was a patient with an extraforaminal lumbar disc herniation at the adjacent level of a previous lumbar instrumentation. We were able to easily identify and remove both the lesions minimizing the surgical approach with no complication and optimal clinical outcome.Discussion and ConclusionOur cases demonstrate the feasibility of application of intraoperative O-arm CT-neuronavigation to the Wiltse approach. In our opinion, this technique helps in minimizing the surgical approach and rapidly identifying the lesion of interest. Further studies are needed to address the effective utility and advantages of intraoperative CT-neuronavigation in this specific surgical scenario.
Developmental toxicity and estrogenic activity of antimicrobial phenolic-branched fatty acids using in silico simulations and in vivo and in vitro bioassay
Authors: Zhang Xinwen , Ngo Helen , Wagner Karen , Fan Xuetong , Wu Changqing 
Published: September 2024

Due to the growing safety and environmental concerns associated with biocides, phenolic-soy branched chain fatty acids (phenolic-soy BCFAs) are synthesized as new bio-based antimicrobial agents. Safety evaluation is essential before the wide adoption of these new antimicrobial products. This study was initiated to evaluate the safety of four phenolic-soy BCFAs (with phenol, thymol, carvacrol, or creosote branches). Methyl-branched iso-oleic acid, phenol, and creosote were included in the study as controls. In silico toxicity simulation tools predicted that the phenolic BCFAs had much higher toxicities to aquatic organisms than free phenolics did, while the opposite was predicted for rats. The developmental toxicity of four phenolic-soy BCFAs was assessed using an in vivo chicken embryonic assay. Results showed that creosote-soy BCFA had much lower mortality rates than creosote at the same dosages. Additionally, creosote-soy BCFA and methyl-branched iso-oleic acid induced minimal estrogenic activity in the concentration range of 10 nM - 1 µM. Carvacrol-soy BCFA treatments significantly increased (p &lt; 0.05) oxidative stress levels with higher thiobarbituric acid reactive substances in the livers of chicken embryos. Altogether, the phenolic-soy BCFAs, especially creosote-soy BCFA, reported in this study are potentially promising and safer bio-based antimicrobial products.
Global Trends and Current Advances in Slow/Controlled-Release Fertilizers: A Bibliometric Analysis from 1990 to 2023
Authors: Li Xianhong , Li Zhonghong 
Published: September 2024

Slow/controlled-release fertilizers (SRFs/CRFs) occupy a critical position in agricultural advancement, enhancing productivity and sustainability by regulating nutrient release, improving fertilizer efficiency, reducing pollution, and promoting lasting agricultural progress. To attain an in-depth understanding of the current landscape, hotspots, and development trends in SRF/CRF research, this study employed the Bibliometrix toolkit in R, VOSviewer, and CiteSpace for the statistical and graphical analysis of pertinent literature in the Web of Science Core Collection (WOSCC) database from 1990 to 2023. In this study, several dimensions were evaluated to assess the research scope and impact, including the quantity of published articles, authorship, citation frequency, keywords, institutional affiliations, publication journals, and source countries. The results indicate a significant increase in scholarly publications related to SRFs/CRFs from 1990 to 2023, totaling 1676 published papers across 77 subject categories. Research activities spanned 69 countries/regions, with China and the USA leading contributions. A total of 1691 research institutions published on SRFs/CRFs, with the University of Florida, the Chinese Academy of Sciences, and China’s Shandong Agricultural University being preeminent. HortScience, Science of the Total Environment, and Communications in Soil Science and Plant Analysis were the top three journals. Keyword co-occurrence and burst analysis disclosed that current research primarily focuses on several key areas: nitrogen (N) use efficiency, the processes of nitrification and denitrification, degradation, the use of phosphate (P) fertilizers, urea, and factors affecting crop growth and quality. The findings revealed several critical areas and trends within the sphere of SRFs/CRFs, with future research specifically directed towards developing cost-effective, efficacious, and environmentally friendly alternatives. Furthermore, future progress will concentrate on addressing the enduring environmental ramifications of SRF/CRF utilization.
Chemical Composition, Antioxidant Capacity, and Anticancerous Effects against Human Lung Cancer Cells of a Terpenoid-Rich Fraction of Inula viscosa
Authors: Seglab Fatiha , Abou Assali Mazen , AlYafei Thoraya , Hassan Hassan , Pinto Diana C. G. A. , Baydoun Safaa , Al Thani Asmaa A. , Shaito Abdullah A. 
Published: September 2024

Inula viscosa is a widely used plant in traditional Mediterranean and Middle Eastern medicine for various illnesses. I. viscosa has been shown to have anticancer effects against various cancers, but its effects against lung cancer have been under limited investigation. At the same time, I. viscosa is rich in terpenoids whose anti-lung cancer effects have been poorly investigated. This study aimed to examine the potential anticancer properties of methanolic and aqueous extracts of stems and leaves of I. viscosa and its terpenoid-rich fraction against human lung cancer A549 cells. Results showed that the methanolic extracts of I. viscosa had significantly higher polyphenol and flavonoid content and radical scavenging capacity than the aqueous extracts. In addition, leaves methanolic extracts (IVLM) caused the highest reduction in viability of A549 cells among all the extracts. IVLM also reduced the viability of human ovarian SK-OV-3, breast MCF-7, liver HepG2, and colorectal HCT116 cancer cells. A terpenoid-rich I. viscosa fraction (IVL DCM), prepared by liquid-liquid separation of IVLM in dichloromethane (DCM), displayed a substantial reduction in the viability of A549 cells (IC50 = 27.8 ± 1.5 µg/mL at 48 h) and the panel of tested cancerous cell lines but was not cytotoxic to normal human embryonic fibroblasts (HDFn). The assessment of IVL DCM phytochemical constituents using GC-MS analysis revealed 21 metabolites, highlighting an enrichment in terpenoids, such as lupeol and its derivatives, caryophyllene oxide, betulin, and isopulegol, known to exhibit proapoptotic and antimetastatic functions. IVL DCM also showed robust antioxidant capacity and decent polyphenol and flavonoid contents. Furthermore, Western blotting analysis indicated that IVL DCM reduced proliferation (reduction of proliferation marker Ki67 and induction of proliferation inhibitor proteins P21 and P27), contaminant with P38 MAP kinase activation, and induced the intrinsic apoptotic pathway (P53/BCL2/BAX/Caspase3/PARP) in A549 cells. IVL DCM also reduced the migration of A549 cells, potentially by reducing FAK activation. Future identification of anticancer metabolites of IVL DCM, especially terpenoids, is recommended. These data place I. viscosa as a new resource of herbal anticancer agents.
Genetic Diversity and Distribution of Haplotypes of Freshwater Eel in Baltic Lakeland Based on Mitochondrial DNA D-Loop and Cytochrome b Sequence Variation
Authors: Oreha Jeļena , Morozova Aleksandra , Garkajs Alberts , Kirjušina Muza , Gavarāne Inese , Kostousov Vladimir G. , Pupins Mihails , Škute Nataļja 
Published: September 2024

The European eel (Anguilla anguilla (L.)) is a unique catadromous euryhaline fish and the only anguillid eel species classified as ‘critically endangered’. The Baltic Lakeland area contains naturally recruited and introduced eels. There is great uncertainty regarding a baseline for the location and number of naturally dispersed eels in the region. Little is known about the genetic structure of the European eel populations in Baltic countries. The estimation of population genetic structure is important for the efficient management of naturally recruited and introduced eels. Two mitochondrial regions were used to investigate the genetic structure within and between eel samples from 11 waterbodies. In this study, new, unique, and widely distributed haplotypes were revealed. The studied eel population in the Baltic Lakeland shows high genetic diversity, which is possibly a result of intensive restocking programs. Sequences characterized for Anguilla rostrata were revealed in both mitochondrial regions. Understanding the genetic structure of eel populations worldwide is crucial for conservation efforts. Eel restocking in waterbodies where natural migration is restricted contributes to diversity loss for the world gene pool of eels.
Two-Decade Changes in the Ciliate Assemblage Feeding Pattern Reflect the Reservoir Nutrient Load
Authors: Macek Miroslav , Vrba Jaroslav , Hejzlar Josef , Řeháková Klára , Jarošík Jiří , Šorf Michal , Šimek Karel 
Published: September 2024

The perception of the importance of ciliate in freshwater has changed dramatically since the “microbial loop” conceptualisation, reflecting methodological attempts. The data from two decades (1994–2018) on the surface (0–3 m) ciliate assemblage in the Slapy reservoir (Vltava River, Czech Republic) during two different nutrient-load defined periods were analysed. We grouped the identified, quantified, and biomass-evaluated ciliates in the quantitative protargol-impregnated preparations according to their feeding behaviour. The sampling median and interquartile range data of the ciliates were plotted; the modelled water age, nutrients, bacteria, heterotrophic nanoflagellates, and Rhodomonas spp. were applied as the main explanatory background variables. We validated the differences between the periods, engaging multivariate analyses. The picoplankton-filtering species dominated the assemblages in an annual mean (halteriids and minute strobilidiids followed by peritrichs). Algae hunting urotrichs, Balanion planctonicum, and nanoplankton filtering tintinnids were significant before the spring phytoplankton peak when a maximum of ciliate biomass reflected mixotrophic nanoplankton filtering pelagostrombidiids. Only there did ciliate biomass tightly follow their quantified prey. Heterotrophic and mixotrophic Askenasia and Lagynophrya were typical raptorial/flagellate-hunting cilates; only Mesodinium spp. reached the maximum during autumn. The observed oligotrophication of the reservoir increased the ciliate assemblage biomass in the surface layer during stratification in concordance with the Plankton Ecology Group (PEG) model.
Notes on the Ecology and Distribution of Species of the Genera of Bondarzewiaceae (Russulales and Basidiomycota) with an Emphasis on Amylosporus
Authors: Hussain Shah , Al-Kharousi Moza , Al-Maqbali Dua’a , Al-Owaisi Arwa A. , Velazhahan Rethinasamy , Al-Sadi Abdullah M. , Al-Yahya’ei Mohamed N. 
Published: September 2024

The family Bondarzewiaceae is an important and diverse group of macrofungi associated with wood as white rotting fungi, and some species are forest tree pathogens. Currently, there are nine genera and approximately 89 species in the family, distributed in tropical, subtropical, and temperate climates. To address the phylogenetic relationships among the genera, a combined ITS-28S dataset was subjected to maximum likelihood (ML), Bayesian inference (BI), and time divergence analyses using the BEAST package. Both ML and BI analyses revealed two major clades, where one major clade consisted of Amylosporus, Stecchericium, and Wrightoporia austrosinensisa. The second major clade is composed of Bondarzewia, Heterobasidion, Gloiodon, Laurilia, Lauriliella, and Wrightoporia, indicating that these genera are phylogenetically similar. Wrightoporia austrosinensisa recovered outside of Wrightoporia, indicating that this species is phylogenetically different from the rest of the species of the genus. Similarly, time divergence analyses suggest that Bondarzewiaceae diversified around 114 million years ago (mya), possibly during the Early Cretaceous Epoch. The genus Amylosporus is well resolved within the family, with an estimated stem age of divergent around 62 mya, possibly during the Eocene Epoch. Further, the species of the genus are recovered in two sister clades. One sister clade consists of species with pileate basidiomata and generative hyphae with clamp connections, corresponding to the proposed section Amylosporus sect. Amylosporus. The other consists of species having resupinate basidiomata and generative hyphae without clamps, which is treated here as Amylosporus sect. Resupinati. We provided the key taxonomic characters, known distribution, number of species, and stem age of diversification of each section. Furthermore, we also described a new species, Amylosporus wadinaheezicus, from Oman, based on morphological characters of basidiomata and multigene sequence data of ITS, 28S, and Tef1-α. With pileate basidiomata and phylogenetic placement, the new species is classified under the proposed A. sect. Amylosporus. An identification key to the known species of Amylosporus is presented. Ecology and distribution of species of the genera in the family are discussed.
Моделювання процесу інфекційного захворювання з урахуванням дифузійних збурень та конвекції в периферійних імунологічних органах
Authors: Барановський Сергій Віталійович , Бомба Андрій Ярославович , Бондар Олена Сергіївна , Ляшко Віктор Сергійович 
Published: August 2024

Для прогнозування динаміки інфекційного захворювання розроблено широкий спектр різноманітних математичних моделей. Як правило, такі моделі не враховують просторові ефекти, пов’язані з нерівномірністю конвекційного перенесення діючих чинників лімфатичною рідиною, зокрема, у внутрішньому середовищі периферійних імунологічних органів. Запропоновано підхід для урахування ефектів нерівномірної конвекції при моделюванні процесів інфекційних захворювань в умовах дифузійних збурень та зосереджених впливів. На основі зведення вихідної модельної сингулярно збуреної задачі із запізненням до послідовності задач без запізнення синтезовано ефективну покрокову процедуру чисельно-асимптотичного наближення розвʼязку як збурення розв’язків відповідних вироджених задач. Для знаходження поля швидкості запропоновано моделювати рух рідини у середовищі імунологічного органа як потенціальну течію у системі джерело–стік. Представлено результати компʼютерного моделювання, які ілюструють особливості впливу конвекції та дифузійного розсіювання на розвиток вірусної інфекції у внутрішньому середовищі імунологічного органа. Модель для умов нерівномірного поля швидкості руху лімфатичної рідини в імунологічному органі забезпечує можливість враховувати наявність зон з різною інтенсивністю знешкодження вірусних елементів. Внаслідок цього можливе виникнення окремих зон, де сила імунної реакції буде недостатньою для знешкодження наявних у них антигенів, що з часом зумовить появу тут нових епіцентрів зараження. Підкреслено, що наявність інструментів для прогнозування динаміки вірусної інфекції з урахуванням нерівномірної конвекції, дифузійних збурень та зосереджених лікувальних впливів у середовищах типу периферійних імунологічних органів важлива при розробці раціональних програм лікування з використанням спеціалізованих експертних систем прийняття рішень.
Perancangan Sistem Pakar Dengan Metode Forward Chaining dan Certainty Factor Untuk Mendeteksi Penyakit Kelinci
Authors: Sajida Mayang , Yuhandri Y , Nurcahyo Gunadi Widi 
Published: September 2024

Pada era teknologi saat ini pemanfaatan kecerdasan buatan atau Artificial Intelligence telah meluas ke berbagai bidang kehidupan manusia. Kecerdasan buatan memiliki cakupan yang sangat luas dan mencakup berbagai aplikasi salah satunya adalah sistem pakar. Sistem pakar merupakan salah satu cabang dari Artificial Intelligence yang dirancang untuk meniru kemampuan dan pengetahuan seorang pakar dalam suatu bidang tertentu yang mempunyai tujuan untuk membuat keputusan yang tepat dan berbasis pengetahuan tersebut. Konteks dunia medis khususnya dalam diagnosis penyakit pada hewan, sistem pakar dapat dimanfaatkan untuk mendiagnosis berbagai penyakit berdasarkan gejala-gejala yang dialami oleh kelinci. Sistem ini dirancang untuk mengidentifikasi 6 jenis penyakit seperti Scabies, Gastroenteritis, Radang Telinga, Radang Mata, Hipocalcium dan Pneumonia, melalui analisis terhadap 24 gejala yang terkait. Penelitian ini bertujuan untuk merancang dan mengembangkan sebuah sistem pakar yang menggunakan metode Forward Chaining (FC) dan Certainty Factor (CF) sebagai teknik utama dalam mendeteksi penyakit pada kelinci. Penelitian ini mengembangkan sistem pakar berbasis online yang dirancang khusus untuk membantu para pemilik kelinci dalam proses diagnosis penyakit yang mungkin diderita oleh kelinci mereka. Sistem ini dibangun dengan tujuan untuk memberikan tingkat kepastian yang tinggi pada hasil diagnosis yang berkaitan dengan penyakit berbahaya pada kelinci. Indikator diagnosis didasari oleh gejala yang didapatkan berdasarkan riwayat diagnosis serta nilai probabilitas mengacu kepada nilai yang diberikan oleh pakar. Berdasarkan pengujian kinerja metode CF dan FC dalam diagnosis mendapatkan tingkat akurasi sebesar 90% berdasarkan data uji. Dengan akurasi tersebut, maka penelitian ini dapat dikatakan berhasil dalam melakukan deteksi penyakit pada kelinci.