bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single nucleotide polymorphisms and genotypes of transient receptor potential ion channel and acetylcholine receptor genes from isolated B lymphocytes in myalgic encephalomyelitis/chronic fatigue syndrome patients
Authors: Marshall-Gradisnik Sonya , Johnston Samantha , Chacko Anu , Nguyen Thao , Smith Peter , Staines Donald 
Published: November 2016

Objective The pathomechanism of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is unknown; however, a small subgroup of patients has shown muscarinic antibody positivity and reduced symptom presentation following anti-CD20 intervention. Given the important roles of calcium (Ca2+) and acetylcholine (ACh) signalling in B cell activation and potential antibody development, we aimed to identify relevant single nucleotide polymorphisms (SNPs) and genotypes in isolated B cells from CFS/ME patients. Methods A total of 11 CFS/ME patients (aged 31.82 ± 5.50 years) and 11 non-fatigued controls (aged 33.91 ± 5.06 years) were included. Flow cytometric protocols were used to determine B cell purity, followed by SNP and genotype analysis for 21 mammalian TRP ion channel genes and nine mammalian ACh receptor genes. SNP association and genotyping analysis were performed using ANOVA and PLINK analysis software. Results Seventy-eight SNPs were identified in nicotinic and muscarinic acetylcholine receptor genes in the CFS/ME group, of which 35 were in mAChM3. The remaining SNPs were identified in nAChR delta (n = 12), nAChR alpha 9 (n = 5), TRPV2 (n = 7), TRPM3 (n = 4), TRPM4 (n = 1) mAChRM3 2 (n = 2), and mAChRM5 (n = 3) genes. Nine genotypes were identified from SNPs in TRPM3 (n = 1), TRPC6 (n = 1), mAChRM3 (n = 2), nAChR alpha 4 (n = 1), and nAChR beta 1 (n = 4) genes, and were located in introns and 3′ untranslated regions. Odds ratios for these specific genotypes ranged between 7.11 and 26.67 for CFS/ME compared with the non-fatigued control group. Conclusion This preliminary investigation identified a number of SNPs and genotypes in genes encoding TRP ion channels and AChRs from B cells in patients with CFS/ME. These may be involved in B cell functional changes, and suggest a role for Ca2+ dysregulation in AChR and TRP ion channel signalling in the pathomechanism of CFS/ME.
Ambulatory Versus In-Hospital Treatment of Proximal Lower-Limb Deep Vein Thrombosis in Adults: A Retrospective Cohort Study
Authors: Mausbach Lisa S. , Avnery Orli , Ellis Martin H. 
Published: November 2016

Background: Complications of deep vein thrombosis (DVT) are related to adequacy of initial anticoagulant therapy. In this study, we analyze consecutive patients with lower-limb proximal DVT and compare the characteristics, treatment, and clinical outcomes of patients receiving entirely ambulatory treatment versus those hospitalized for initial treatment. Methods: This was a retrospective study of consecutive patients with a first proximal lower-limb DVT during a 2-year period. Patients were followed for 90 days. Major end points were all-cause mortality, bleeding requiring hospitalization, and recurrent venous thromboembolism (VTE). Events were determined for patients who were hospitalized versus those treated on an entirely ambulatory basis. Results: A total of 236 patients were included in the study. Of these, 147 patients were hospitalized and 89 patients received ambulatory treatment. There were 20 fatalities—18 in-hospital and 2 in-ambulatory patients ( P = .008). By multivariable Cox regression analysis, the presence of active cancer (hazard ratio [HR] = 5.44; confidence interval [CI]: 2.16-13.7; P = .001), age (HR = 1.06; CI: 1.02-1.1; P = .001), and hospitalization (HR = 5.73; CI: 1.33-24.69; P = .019) were associated with death. Eight hospitalized and 2 ambulatory patients required readmission because of bleeding. Age was the only variable associated with bleeding (HR = 1.10; CI: 1.03-1.18; P = .004). There were no recurrent VTE events. Conclusion: In this study of routine management of proximal DVT, we demonstrate that patients suitable for ambulatory care are adequately identified by physicians and may be treated with equal safety and efficacy to hospitalized patients.
Medial Malleoli Fractures: Clinical Comparison Between Newly Designed Sled Device and Conventional Screws
Authors: Maniar Hemil , Kempegowda Harish , Tawari Akhil A. , Rutter Michael R. , Borade Amrut , Cush Gerard , Horwitz Daniel S. 
Published: November 2016

Background. The Trimed Medial Malleolar Sled is a newer device designed to treat medial malleolus fracture. The purpose of this study was to compare the outcome of medial malleolar fractures treated with the sled and conventional malleolar screws. Methods. After obtaining an institutional review board approval, we conducted a retrospective study to identify all skeletally mature patients who sustained an ankle fracture with medial malleolar involvement treated with the sled and we identified a matched cohort treated with conventional malleolar screws. The patients were divided into 2 groups: group A included patients treated with malleolar screws and group B included patients treated with the sled device. The outcomes measured included rate of union, implant removal, and pain over the implant site. Results. Eighty-five medial malleolar ankle fractures were divided into 2 groups: group A included patients (n = 64) treated with malleolar screws and group B included patients (n = 21) treated with the sled device. In group A (n = 64), 62 patients (96.8%) achieved radiological union with a mean union rate of 11 weeks and 10 (15%) patients underwent repeat surgery for implant removal of which 3 patients (4.6%) had pain specifically over the medial implant. In group B (n = 21), all of the patients (100%) achieved radiological union with a mean union rate of 10.8 weeks and 3 patients (14.2%) underwent repeat surgery of which 1 (4.7%) was related to the medial pain. There is no significant difference between the groups for the outcomes measured, including rate of union (  P = .93), visual analog scale score for pain (  P = .07), implant removal (  P = .41), and pain over the implant site (  P = .88). Conclusion. Based on the data from our study, we conclude that there are no major differences between the sled devices and conventional screws relating to union rate and complications. Levels of Evidence: Level III: Observational study
An incomplete trafficking defect to the cell-surface leads to paradoxical thrombocytosis for human and murine MPL P106L
Authors: Favale Fabrizia , Messaoudi Kahia , Varghese Leila N. , Boukour Siham , Pecquet Christian , Gryshkova Vitalina , Defour Jean Philippe , Albu Roxana-Irina , Bluteau Olivier , Ballerini Paola , Leverger Guy , Plo Isabelle , Debili Najet , Raslova Hana , Favier Remi , Constantinescu Stefan N. , Vainchenker William 
Published: November 2016

Key Points
MPL P106L induces thrombocytosis due to an incomplete trafficking defect that allows very low cell-surface levels. The P106L mutation uncouples MPL signaling from its THPO clearance functions.
Intellectual Property Strategy and the Long Tail: Evidence from the Recorded Music Industry
Authors: Zhang Laurina 
Published: November 2016

Digitization has impacted firm profitability in many media industries by lowering the cost of copying and sharing creative works. I examine the impact of digital rights management (DRM), a prevalent strategy used by firms in media industries to address piracy concerns, on music sales. I exploit a natural experiment, where different labels remove DRM from their entire catalogue of music at different times, to examine whether relaxing an album’s sharing restrictions increases sales. Using a large sample of albums from all four major record labels, I find that removing DRM increases digital music sales by 10%, but relaxing sharing restrictions does not impact all albums equally. It increases the sales of lower-selling albums (i.e., the “long tail”) significantly (40%) but does not benefit top-selling albums. These results suggest that reducing search costs facilitates the discovery of niche products. This paper was accepted by Lee Fleming, entrepreneurship and innovation.
Research roundup: November 2016
Authors: Greener Mark 
Published: November 2016

In brief The percentage of children with special educational needs is higher among those conceived between January and March than at other times of the year Adverse events appear to be consistently under-reported in published studies Morning sickness may be associated with fewer pregnancy losses Vitamin B3: Maternal levels of nicotinamide seem to influence the likelihood that their children will develop atopic eczema Potentially inappropriate prescribing appears to be common in older people on polypharmacy
Mathematical Modeling of Carbon Nanotubes Formation in Fluidized Bed Chemical Vapor Deposition
Authors: Danafar Firoozeh , Elnashaie Said S. , Hashemipour Hassan , Rostamizadeh Mohammad Ali 
Published: November 2016

Abstract
This study investigates mathematical modeling of carbon nanotubes (CNTs) formation on catalyst particulate in a fluidized bed chemical vapor deposition (FBCVD) reactor. The mass of CNTs formed corresponds to the catalyst activity directly. The catalyst deactivation occurs as active sites are occupied by CNTs and thus causes unsteady state behavior of the process. The effects of catalyst loading (as bed height) as well as reaction temperature on the reaction progressing were investigated. The model, validated with our experimental data, indicates a good accuracy to predict the yield of CNTs formation for a given operating conditions. The model presented also can predict the optimized time as well as the suitable amount of catalyst loading to produce CNTs for a given reactor conditions.
Prime Repetition and Korean EFL Learners’ Comprehension and Production of Passives
Authors: Kim YouJin , McDonough Kim 
Published: November 2016

AbstractThe occurrence of structural priming in second language (L2) speech production has received increased attention over the last decade (McDonough and Trofimovich, 2009; Trofimovich and McDonough, 2011). Although L2 researchers have implemented a variety of priming methods, few studies to date have investigated whether repeating prime sentences affects L2 speakers’ understanding and use of target structures. Therefore, the current study investigated whether prime repetition facilitates English L2 speakers’ comprehension and production of passive constructions. Korean EFL learners (
Simultaneously proximinal subspaces
Authors: Rao T. S. S. R. K. 
Published: November 2016

Abstract
In this paper we study closed subspaces of Banach spaces that admit
relative Chebyshev centers for all bounded subsets of the space. We
exhibit new classes of spaces which have this property and study
stability results similar to the ones studied in the literature in
the context of proximinal subspaces and Chebyshev centers. For the space

C
⁢

(
K
)

${C(K)}$

of continuous functions on a compact set K, we show that a
closed subspace of finite codimension has relative Chebyshev centers
for all bounded sets in

C
⁢

(
K
)

${C(K)}$

if and only if it is a strongly
proximinal subspace.
Ritz-least squares method for finding a control parameter in a one-dimensional parabolic inverse problem
Authors: Noei Khorshidi Meisam , Yousefi Sohrab Ali 
Published: November 2016

Abstract
An inverse problem concerning a diffusion equation with source control
parameter is considered. The approximation of the problem is based on the
Ritz method with satisfier function. The Ritz method together with the least squares
approximation (Ritz-least squares
method) are utilized to reduce the inverse problem to
the solution of algebraic equations. We extensively discuss the
convergence of the method and finally present illustrative
examples to demonstrate validity and applicability of the new
technique.
A new approach to nonlinear singular integral operators depending on three parameters
Authors: Uysal Gumrah 
Published: November 2016

Abstract
In this paper, we present some theorems on weighted approximation by two dimensional nonlinear singular integral operators in the following form:

T
λ

(
f
;
x
,
y
)
=

∬

R

2

K
λ

(
t
−
x
,
s
−
y
,
f
(
t
,
s
)
)
d
s
d
t
,

(
x
,
y
)
∈

R

2

,
λ
∈
Λ
,

$${T_\lambda }(f;x,y) = \iint\limits_{{\mathbb{R}^2}}K_\lambda {(t - x,s - y,f(t,s))dsdt,\;(x,y) \in {\mathbb{R}^2},\lambda \in \Lambda ,}$$

where Λ is a set of non-negative numbers with accumulation point λ0.
Mental Health Conditions and Medical and Surgical Hospital Utilization
Authors: Doupnik Stephanie K. , Lawlor John , Zima Bonnie T. , Coker Tumaini R. , Bardach Naomi S. , Hall Matt , Berry Jay G. 
Published: November 2016

OBJECTIVE:
Mental health conditions are prevalent among children hospitalized for medical conditions and surgical procedures, but little is known about their influence on hospital resource use. The objectives of this study were to examine how hospitalization characteristics vary by presence of a comorbid mental health condition and estimate the association of a comorbid mental health condition with hospital length of stay (LOS) and costs.

METHODS:
Using the 2012 Kids’ Inpatient Database, we conducted a retrospective, nationally representative, cross-sectional study of 670 161 hospitalizations for 10 common medical and 10 common surgical conditions among 3- to 20-year-old patients. Associations between mental health conditions and hospital LOS were examined using adjusted generalized linear models. Costs of additional hospital days associated with mental health conditions were estimated using hospital cost-to-charge ratios.

RESULTS:
A comorbid mental health condition was present in 13.2% of hospitalizations. A comorbid mental health condition was associated with a LOS increase of 8.8% (from 2.5 to 2.7 days, P &lt; .001) for medical hospitalizations and a 16.9% increase (from 3.6 to 4.2 days, P &lt; .001) for surgical hospitalizations. For hospitalizations in this sample, comorbid mental health conditions were associated with an additional 31 729 (95% confidence interval: 29 085 to 33 492) hospital days and $90 million (95% confidence interval: $81 to $101 million) in hospital costs.

CONCLUSIONS:
Medical and surgical hospitalizations with comorbid mental health conditions were associated with longer hospital stay and higher hospital costs. Knowledge about the influence of mental health conditions on pediatric hospital utilization can inform clinical innovation and case-mix adjustment.
Fuerza Pública, negociaciones de paz y posacuerdo en Colombia
Authors: Ramírez Charry Edwin Jair 
Published: November 2016

Cruz Rodríguez, E. (2016). Fuerza Pública, negociaciones de paz y posacuerdo en Colombia. Bogotá: desde abajo, 138págs.Edwin Cruz Rodríguez, candidato a Doctor en Estudios Políticos y Relaciones Internacionales e investigador de la Universidad Nacional de Colombia acaba de publicar una obra de gran relevancia a propósito del conflicto armado interno en Colombia y las actuales negociaciones de paz en La Habana. El texto evidencia avances y retos significativos para la búsqueda del fin del conflicto representado entre el Estado y las Fuerzas Armadas Revolucionarias de Colombia (FARC).
PENGARUH PENAMBAHAN KALSIUM KARBONAT (CaCO3) DALAM MEDIA PEMELIHARAAN IKAN RAINBOW KURUMOI (Melanotaenia parva) TERHADAP PERTUMBUHAN BENIH DAN PRODUKSI LARVANYA
Authors: Kadarini Tutik , Musthofa Siti Zuhriyyah , Subandiyah Siti , Priono Bambang 
Published: November 2016

Upaya peningkatan produksi ikan rainbow Kurumoi (Melanotaenia parva) dapat dilakukan melalui pendekatan lingkungan, salah satunya yaitu dengan manipulasi kesadahan air dalam lingkungan budidaya. Toleransi terhadap kesadahan setiap jenis dan ukuran/umur berbeda. Ikan rainbow dapat hidup dengan baik pada kesadahan air berkisar antara 50-250 mg/L CaCO3. Nilai kesadahan air dapat ditingkatkan di antaranya dengan penambahan kalsium karbonat (CaCO3). Penelitian ini bertujuan untuk mengetahui pengaruh penambahan CaCO3 dalam media pemeliharaan terhadap pertumbuhan benih ikan rainbow kurumoi dan produksi larvanya. Penelitian ini terdiri dari dua tahap yaitu: pembesaran dan pemijahan. Perlakuan yang diberikan pada kedua tahap penelitian adalah penambahan CaCO3 yang berbeda pada media pemeliharaannya, yaitu: (A) tanpa penambahan (kontrol), (B) 30 mg/L media, (C) 60 mg/L media, (D) 90 mg/L media, dan (E) 120 mg/L media, dengan tiga kali ulangan. Parameter yang diamati adalah pertumbuhan, sintasan benih dan induk, produksi larva, kualitas air, dan glukosa darah. Hasil penelitian menunjukkan bahwa penambahan CaCO3 dengan dosis 60-120 mg/L adalah yang terbaik untuk pertumbuhan benih rainbow kurumoi (tahap pembesaran). Perlakuan ini menghasilkan pertambahan bobot rata-rata 0,81±0,11 –0,84±0,32 g; pertambahan panjang standar rata-rata 0,81±0,12–0,92±0,17 cm; dan sintasan 96,67%-97,5%. Sedangkan untuk produksi larva penambahan CaCO3 dengan dosis 30-60 mg/L menghasilkan larva rata-rata 113±36,4–160±105,8 ekor; dan sintasan induk 91,67±28,9–100±0%.
PENGEMBANGAN METODE ELISA UNTUK MENDETEKSI RESPON IMUN SPESIFIK PADA IKAN NILA (Oreochromis niloticus) YANG DIVAKSINASI TERHADAP Aeromonas hydrophila DAN Streptococcus agalactiae
Authors: Sumiati Tuti , Sukenda Sukenda , Nuryati Sri , Lusiastuti Angela Mariana 
Published: November 2016

Ko-infeksi bakteri Aeromonas hydrophila dan Streptococcus agalactiae rentan terjadi pada budidaya nila, sehingga pencegahan menggunakan vaksin koktail yang mengandung kedua bakteri tersebut merupakan langkah yang tepat untuk pengendaliannya. Penelitian ini bertujuan untuk menganalisa respon imun spesifik (antibodi) serum ikan nila yang divaksinasi dengan vaksin koktail A. hydrophila dan S. agalactiae dengan menggunakan ELISA. Penelitian dilakukan dalam dua tahap, tahap pertama adalah optimalisasi ELISA, yang terdiri atas persiapan antigen dan penentuan konsentrasi optimum antigen untuk deteksi antibodi dan tahap kedua adalah mendeteksi antibodi pasca vaksinasi ikan nila. Hasil penelitian menunjukkan bahwa konsentrasi optimum antigen A. hydrophila dan S. agalactiae untuk penentuan antibodi dalam ikan nila masing-masing sebesar 10 μg/mL. Konsentrasi tersebut dapat digunakan untuk mendeteksi antibodi serum pada ikan nila setelah diberi vaksin koktail A. hydrophila dan S. agalactiae. Titer antibodi ikan nila pada minggu kedua sampai pada minggu kelima pada kelompok vaksinasi secara signifikan lebih tinggi dibanding kontrol (P<0,05).
KERAGAAN WARNA IKAN CLOWN BIAK (Amphiprion percula) POPULASI ALAM DAN BUDIDAYA BERDASARKAN ANALISIS GAMBAR DIGITAL
Authors: Kusumah Ruby Vidia , Cindelaras Sawung , Prasetio Anjang Bangun 
Published: November 2016

Penelitian ini bertujuan untuk mengkaji keragaan warna ikan clown Biak (Amphiprion percula) populasi alam dan budidaya berdasarkan analisis gambar digital sebagai dasar upaya pemuliaannya. Gambar digital diambil dari koleksi ikan clown Biak Balai Besar Perikanan Budidaya Laut Lampung, Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, Bali; serta pengumpul ikan hias di Denpasar, Bali menggunakan kamera digital Canon EOS 600D. Pola warna dikarakterisasi secara visual terhadap variasi strip hitam dan putih pada dasar badan oranye, jenis warna dianalisis menggunakan ImageJ 1.49s, persentase penutupan warna dilakukan dengan Adobe Photoshop CS5. Pola warna dikarakterisasi oleh strip hitam tebal, tipis, gelap, pudar, terputus, bergabung, serta strip putih normal, pelana, spot, melebar, dan terputus. Warna hitam alam dikarakterisasi oleh hue (H): 300-60º, saturation (S): 8%-56%, brightness (B): 3%-19%, sedangkan budidaya H: 300-23º, S: 9%-71%, B: 4%-20%. Warna oranye alam H: 19-33º, S: 88%-98%, B: 47%-85%, dan budidaya H: 14-29º, S: 86%-99%, B: 38%-82%. Warna putih alam H: 36-270º; S: 1%-13%, B: 66%-88%, dan budidaya H: 0-229º, S: 0%-14%, B: 55%-87%. Persentase penutupan warna badan didominasi warna oranye dengan rata-rata 45% untuk populasi alam dan 57% untuk populasi budidaya. Keragaan warna ikan clown Biak dapat diarahkan pada pembentukan strain misbar, picasso, spot (domino), dan onyx. Metode analisis gambar digital sangat potensial digunakan untuk analisis keragaan warna ikan hias.
DISTRIBUSI RUMPUT LAUT ALAM BERDASARKAN KARAKTERISTIK DASAR PERAIRAN DI KAWASAN RATAAN TERUMBU LABUHANBUA, NUSA TENGGARA BARAT: STRATEGI PENGELOLAAN UNTUK PENGEMBANGAN BUDIDAYA
Authors: Erlania Erlania , Radiarta I Nyoman 
Published: November 2016

Keberadaan rumput laut di alam sangat dipengaruhi oleh karakteristik substrat dasar perairan. Penelitian ini bertujuan untuk menganalisis distribusi dan hubungan tutupan rumput laut alam dengan komponen substrat dasar perairan di sepanjang pantai Labuhanbua, Kabupaten Sumbawa. Pengumpulan data lapangan pada 46 titik pengamatan menggunakan transek kuadrat ukuran 1 m x 1 m yang ditempatkan sepanjang transek garis yang disebar tegak lurus terhadap garis pantai dengan jarak masing-masing sekitar 50 m. Parameter yang diamati adalah: persentase tutupan dasar perairan yang terdiri atas tutupan pasir, karang hidup, pecahan karang, lumpur, batu karang, lamun, dan rumput laut (total tutupan 100%). Pengambilan sampel substrat dasar perairan juga dilakukan pada beberapa titik pengamatan untuk analisa parameter P2O5, N total, C organik total, dan tekstur substrat 3-fraksi: pasir, liat, dan debu. Analisis data dilakukan secara spasial dan statistik (analisis klaster dan deskriptif). Hasil penelitian memperlihatkan bahwa jenis Rhodophyta banyak ditemukan di kawasan dengan substrat dominan berlumpur; Chlorophyta pada substrat dengan asosiasi antara pasir, karang hidup, dan batu karang; sedangkan Phaeophyta lebih banyak ditemukan pada substrat dasar yang didominasi oleh pecahan karang. Secara keseluruhan, jenis-jenis dari kelompok Phaeophyta memiliki tutupan yang relatif jauh lebih tinggi dibandingkan Chlorophyta dan Rhodophyta. Strategi pengelolaan sumberdaya rumput laut alam dan pengembangan budidayanya dapat diimplementasikan melalui beberapa langkah penting, yaitu pengaturan pemanenan rumput laut alam, identifikasi jenis prospektif dan potensinya, pengembangan teknologi budidaya adaptif dan spesifik, serta studi kesesuaian lahan untuk pengembangan budidaya rumput laut potensial.
EFEKTIVITAS KINERJA MEDIA BIOFILTER DALAM SISTEM RESIRKULASI TERHADAP KUALITAS AIR UNTUK PERTUMBUHAN DAN SINTASAN IKAN RED RAINBOW (Glossolepis incisus Weber)
Authors: Nurhidayat Nurhidayat , Nirmala Kukuh , Djokosetyanto D. 
Published: November 2016

Penelitian ini bertujuan untuk mengetahui efektivitas kinerja media biofilter zeolit dan bioball dalam perbaikan kualitas air yang akan meningkatkan pertumbuhan dan sintasan ikan red rainbow (Glossolepis incisus Weber) pada sistem resirkulasi. Wadah percobaan berupa akuarium dengan ukuran 100 cm x 60 cm x 40 cm sebanyak 24 buah, masing-masing ditebar ikan sebanyak 60 ekor/akuarium. Ikan uji yang digunakan dengan bobot awal 0,71 g dan panjang 3,51 cm. Perlakuan yang digunakan dalam percobaan adalah media biofilter: A. zeolit 100%, B. zeolit 75% + bioball 25%, C. zeolit 50% + bioball 50%, D. zeolit 25% + bioball 75%, E. bioball 100%, dan F. kontrol. Pakan yang digunakan adalah pelet komersial, diberikan 5% dari bobot total ikan yang diberikan sebanyak tiga kali (pagi, siang, dan sore). Percobaan disusun dalam Rancangan Acak Lengkap (RAL), dengan enam perlakuan yang diulang sebanyak empat kali. Hasil pengamatan perlakuan media biofilter zeolit 75% + bioball 25% memberikan kualitas air terbaik dengan kualitas baik (kategori IV), dengan efektivitas oksidasi amonia sebesar 95,03±0,31% dan biomassa bakteri non patogen (nitrifikasi) dengan jumlah koloni (8,6±1,91) x 106. Pertumbuhan panjang total dan sintasan yang diperoleh 66,67% (2,34 cm) dan 96,67%.