bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of an ionic‐liquid‐based polymer monolithic column and its application in the fractionation of proteins from complex biosamples
Authors: Zhang Doudou , Zhang Qian , Bai Ligai , Han Dandan , Liu Haiyan , Yan Hongyuan 
Published: January 2018

AbstractAn ionic‐liquid‐based polymer monolithic column was synthesized by free radical polymerization within the confines of a stainless‐steel column (50 mm × 4.6 mm id). In the processes, ionic liquid and stearyl methacrylate were used as dual monomers, ethylene glycol dimethacrylate as the cross‐linking agent, and polyethylene glycol 200 and isopropanol as co‐porogens. Effects of the prepolymerization solution components on the properties of the resulting monoliths were studied in detail. Scanning electron microscopy, nitrogen adsorption–desorption measurements, and mercury intrusion porosimetry were used to investigate the morphology and pore size distribution of the prepared monoliths, which showed that the homemade ionic‐liquid‐based monolith column possessed a relatively uniform macropore structure with a total macropore specific surface area of 44.72 m2/g. Compared to a non‐ionic‐liquid‐based monolith prepared under the same conditions, the ionic‐liquid‐based monolith exhibited excellent selectivity and high performance for separating proteins from complex biosamples, such as egg white, snailase, bovine serum albumin digest solution, human plasma, etc., indicating promising applications in the fractionation and analysis of proteins from the complex biosamples in proteomics research.
Chitosan‐Based Thermo/pH Double Sensitive Hydrogel for Controlled Drug Delivery
Authors: Bai Xiaoyu , Bao Zixian , Bi Shichao , Li Yang , Yu Xiaoping , Hu Shihao , Tian Meiping , Zhang Xin , Cheng Xiaojie , Chen Xiguang 
Published: January 2018

AbstractA series of thermo/pH sensitive N‐succinyl hydroxybutyl chitosan (NSHBC) hydrogels with different substitution degrees of succinyl are prepared for drug delivery. Rheology analysis shows that the gelation temperature of NSHBC hydrogels is 3.8 °C higher than that of hydroxybutyl chitosan (HBC) hydrogels. A model drug bovine serum albumin (BSA) is successfully loaded and released. NSHBC hydrogels show excellent pH sensitivity drug release behaviors. After incubation for 24 h, 93.7% of BSA is released from NSHBC hydrogels in phosphate buffer saline (PBS) (pH 7.4), which is significantly greater than that of 24.6% at pH 3.0. In contrast, the release rate of BSA from HBC is about 70.0% at pH 3.0 and 7.4. Thus, these novel hydrogels have the prominent merits of high adaptability to soluble drugs and pH sensitivity triggered release, indicating that NSHBC hydrogels have promising applications in oral drug delivery.
Biological Activity of an Injectable Biphasic Calcium Phosphate/PMMA Bone Cement for Induced Osteogensis in Rabbit Model
Authors: Zhang Xiashiyao , Kang Ting , Liang Peiqing , Tang Yong , Quan Changyun 
Published: January 2018

AbstractPolymethylmethacrylate (PMMA) bone cement is widely used in repair of vertebral fracture because of its good biomechanical properties and fast curing. However, the bioinertness of PMMA cement may cause interfacial loosening, fatigue, fracture, and ultimate failure. In this study, biphasic calcium phosphate (BCP) is introduced into PMMA cement to prepare an injectable composite bone cement (BCPx/PMMA) and the content of BCP is optimized to achieve appropriate rate of absorption that matches the bone regeneration. The compressive strength of BCPx/PMMA bone cement is found to comply with the International Standardization Organization standard 5833, and can promote biomineralization as well as adhesion, proliferation, and osteogenic differentiation of Sprague‐Dawley rat bone marrow mesenchymal stem cells in vitro. Furthermore, in vivo test performed on a rabbit radius defect model demonstrates that the presence of BCP can significantly improve the osteogenic efficacy of PMMA cement. Therefore, it is anticipated that BCPx/PMMA bone cement, as a promising injectable biomaterial, is of great potential in bone tissue regeneration.
The Tunable Porous Structure of Gelatin–Bioglass Nanocomposite Scaffolds for Bone Tissue Engineering Applications: Physicochemical, Mechanical, and In Vitro Properties
Authors: Arabi Neda , Zamanian Ali , Rashvand Sarvenaz N. , Ghorbani Farnaz 
Published: January 2018

AbstractUnidirectional freeze‐casting method is used to fabricate gelatin–bioglass nanoparticles (BGNPs) scaffolds. Transmission electron microscopy (TEM) images show that sol–gel prepared BGNPs are distributed throughout the scaffold with diameters of less than 10 nm. Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetric are used to evaluate the physicochemical properties of BGNPs. Scanning electron microscopy (SEM) micrographs present an oriented porous structure and a homogeneous distribution of BGNPs in the gelatin matrix. The lamellar‐type structure indicates an improvement of mechanical strength and absorption capacity of the scaffolds. Increasing the concentration of BGNPs from 0 to 50 wt% have no noticeable effect on pore orientation, but decreases porosity and pore size distribution. Increase in BGNPs content improves the compressive strength. The absorption and biodegradation rate reduces with augmentation in BGNPs concentration. Bioactivity is evaluated through apatite formation after immersion of the nanocomposites in simulated body fluid and is verified by SEM–energy‐dispersive X‐ray spectroscopy (EDS), an element map analysis, X‐ray powder diffractometer, and FTIR spectrum. SEM images and methyl thiazolyl tetrazolium assay confirm the biocompatibility of scaffolds and the supportive behavior of nanocomposites in cellular spreading. The results show that gelatin–(30 wt%)bioglass nanocomposites have incipient physicochemical and biological properties.
Clinical and Vitamin Response to a Short‐Term Multi‐Micronutrient Intervention in Brazilian Children and Teens: From Population Data to Interindividual Responses
Authors: Mathias Mariana Giaretta , Coelho‐Landell Carolina de Almeida , Scott‐Boyer Marie‐Pier , Lacroix Sébastien , Morine Melissa J. , Salomão Roberta Garcia , Toffano Roseli Borges Donegá , Almada Maria Olímpia Ribeiro do Vale , Camarneiro Joyce Moraes , Hillesheim Elaine , de Barros Tamiris Trevisan , Camelo‐Junior José Simon , Campos Giménez Esther , Redeuil Karine , Goyon Alexandre , Bertschy Emmanuelle , Lévêques Antoine , Oberson Jean‐Marie , Giménez Catherine , Carayol Jerome , Kussmann Martin , Descombes Patrick , Métairon Slyviane , Draper Colleen Fogarty , Conus Nelly , Mottaz Sara Colombo , Corsini Giovanna Zambianchi , Myoshi Stephanie Kazu Brandão , Muniz Mariana Mendes , Hernandes Lívia Cristina , Venâncio Vinícius Paula , Antunes Lusania Maria Greggi , da Silva Rosana Queiroz , Laurito Taís Fontellas , Rossi Isabela Ribeiro , Ricci Raquel , Jorge Jéssica Ré , Fagá Mayara Leite , Quinhoneiro Driele Cristina Gomes , Reche Mariana Chinarelli , Silva Paula Vitória Sozza , Falquetti Letícia Lima , da Cunha Thaís Helena Alves , Deminice Thalia Manfrin Martins , Tambellini Tâmara Hambúrguer , de Souza Gabriela Cristina Arces , de Oliveira Mariana Moraes , Nogueira‐Pileggi Vicky , Matsumoto Marina Takemoto , Priami Corrado , Kaput Jim , Monteiro Jacqueline Pontes 
Published: January 2018

ScopeMicronutrients are in small amounts in foods, act in concert, and require variable amounts of time to see changes in health and risk for disease. These first principles are incorporated into an intervention study designed to develop new experimental strategies for setting target recommendations for food bioactives for populations and individuals.Methods and resultsA 6‐week multivitamin/mineral intervention is conducted in 9–13 year olds. Participants (136) are (i) their own control (n‐of‐1); (ii) monitored for compliance; (iii) measured for 36 circulating vitamin forms, 30 clinical, anthropometric, and food intake parameters at baseline, post intervention, and following a 6‐week washout; and (iv) had their ancestry accounted for as modifier of vitamin baseline or response. The same intervention is repeated the following year (135 participants). Most vitamins respond positively and many clinical parameters change in directions consistent with improved metabolic health to the intervention. Baseline levels of any metabolite predict its own response to the intervention. Elastic net penalized regression models are identified, and significantly predict response to intervention on the basis of multiple vitamin/clinical baseline measures.ConclusionsThe study design, computational methods, and results are a step toward developing recommendations for optimizing vitamin levels and health parameters for individuals.
Cross‐vendor harmonization of T2‐relaxation‐under‐spin‐tagging (TRUST) MRI for the assessment of cerebral venous oxygenation
Authors: Jiang Dengrong , Liu Peiying , Li Yang , Mao Deng , Xu Cuimei , Lu Hanzhang 
Published: January 2018

PurposeCerebral venous oxygenation (Yv) is an important physiological parameter and has potential clinical application in many brain diseases. T2‐relaxation‐under‐spin‐tagging (TRUST) is a commonly used MRI method to measure Yv. Harmonization of this technique across MRI vendors is important for dissemination and multicenter studies of brain oxygenation and metabolism as a disease biomarker.MethodsTRUST pulse sequence components and imaging parameters were carefully matched between two major MRI vendors, Philips and Siemens. Each subject (N = 10) was scanned on both scanners within a 2.5‐h period. On each scanner, the subject was scanned in two sessions to assess intersession reproducibility. A hyperoxia challenge was also included in both sessions and on both scanners to evaluate the sensitivity of the technique to Yv changes. Measured Yv values, confidence interval of Yv estimates (
), as well as intrasession and intersession coefficient of variation (CoV) of Yv, were compared between the two scanners.ResultsYv measured on the two vendors were highly compatible and strongly correlated (R2 = 0.957). Yv changes associated with hyperoxia challenge were significant on both scanners (P < 0.001) and were also correlated across scanners (P = 0.007). Intrasession and intersession CoV of measured Yv were less than 3% and showed no difference between scanners.
were less than 1% on both scanners and showed no difference between scanners when echo times were matched on the two scanners.ConclusionThis work suggests that harmonized TRUST MRI can yield highly compatible Yv measurements across different vendors. Magn Reson Med 80:1125–1131, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
Permissive or Trophic Enteral Nutrition and Full Enteral Nutrition Had Similar Effects on Clinical Outcomes in Intensive Care: A Systematic Review of Randomized Clinical Trials
Authors: Silva Camila F. A. , de Vasconcelos Simone G. , da Silva Thales A. , Silva Flávia M. 
Published: January 2018

AbstractThe aim of this study was to systematically review the effect of permissive underfeeding/trophic feeding on the clinical outcomes of critically ill patients. A systematic review of randomized clinical trials to evaluate the mortality, length of stay, and mechanical ventilation duration in patients randomized to either hypocaloric or full‐energy enteral nutrition was performed. Data sources included PubMed and Scopus and the reference lists of the articles retrieved. Two independent reviewers participated in all phases of this systematic review as proposed by the Cochrane Handbook, and the review was reported according to Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines. A total of 7 randomized clinical trials that included a total of 1,717 patients were reviewed. Intensive care unit length of stay and mechanical ventilation duration were not statistically different between the intervention and control groups in all randomized clinical trials, and mortality rate was also not different between the groups. In conclusion, hypocaloric enteral nutrition had no significantly different effects on morbidity and mortality in critically ill patients when compared with full‐energy nutrition. It is still necessary to determine the safety of this intervention in this group of patients, the optimal amount of energy provided, and the duration of this therapy.
High‐performance geometric nonlinear analysis with the unsymmetric 4‐node, 8‐DOF plane element US‐ATFQ4
Authors: Li Zhi , Cen Song , Wu Cheng‐Jin , Shang Yan , Li Chen‐Feng 
Published: January 2018

SummaryA recent unsymmetric 4‐node, 8‐DOF plane element US‐ATFQ4, which exhibits excellent precision and distortion‐resistance for linear elastic problems, is extended to geometric nonlinear analysis. Since the original linear element US‐ATFQ4 contains the analytical solutions for plane pure bending, how to modify such formulae into incremental forms for nonlinear applications and design an appropriate updated algorithm become the key of the whole job. First, the analytical trial functions should be updated at each iterative step in the framework of updated Lagrangian formulation that takes the configuration at the beginning of an incremental step as the reference configuration during that step. Second, an appropriate stress update algorithm in which the Cauchy stresses are updated by the Hughes‐Winget method is adopted to estimate current stress fields. Numerical examples show that the new nonlinear element US‐ATFQ4 also possesses amazing performance for geometric nonlinear analysis, no matter whether regular or distorted meshes are used. It again demonstrates the advantages of the unsymmetric finite element method with analytical trial functions.
Targeted therapy for infants with diencephalic syndrome: A case report and review of management strategies
Authors: Wagner Lars M. , Myseros John S. , Lukins Douglas E. , Willen Christi M. , Packer Roger J. 
Published: January 2018

AbstractYoung children with emaciation caused by a hypothalamic glioma are considered to have diencephalic syndrome (DS), which is often poorly controlled with conventional treatment. We describe an infant with DS whose tumor progressed following chemotherapy. Biopsy was performed for molecular testing and demonstrated a BRAF fusion. Treatment with the MEK inhibitor trametinib for 18 months resulted in reduction of tumor size, normalization of his weight curve, and marked neurodevelopmental improvement. Our results build on earlier reports of using targeted agents for low‐grade glioma, and we review the evolving management strategy for such patients in the era of precision medicine.
High‐voltage p‐type PERC solar cells with anchored plating and hydrogenation
Authors: Ciesla Alison , Chen Ran , Wang Sisi , Ji Jingjia , Shi Zhengrong , Mai Ly , Chan Catherine , Hallam Brett , Chong CheeMun , Wenham Stuart , Green Martin 
Published: January 2018

AbstractA common concern regarding plated contacts to solar cells is the adhesion strength. In this work, laser‐formed anchor points have been applied to Suntech Power's PLUTO passivated emitter and rear cells. Voltages as high as 696 mV have been achieved, showing the ability of a laser‐doped selective emitter at the front surface and localized contacts at the rear when combined with the hydrogenation of defects to reduce the device dark saturation current to well below current norms for commercial passivated emitter and rear cells. The simple hydrogen passivation process applied during sintering appears to facilitate the high voltages by significantly reducing recombination associated with the p‐type Cz wafer and laser‐induced defects formed during laser doping. The same hydrogenation process almost entirely eliminates the damage caused by laser ablation in forming the anchor points. With 50% anchor point coverage (more than necessary for adhesion equivalent to or stronger than screen‐printed contacts), an average VOC of 693 mV was achieved, with an average current of 40.5 mA/cm2, average device efficiency of 20.2%, and a single best cell of 20.5% efficiency. These cells also exhibit excellent contact adhesion and pass all thermal cycling and damp‐heat testing according to IEC 61215.
Phlorotannin supplement decreases wake after sleep onset in adults with self‐reported sleep disturbance: A randomized, controlled, double‐blind clinical and polysomnographic study
Authors: Um Min Young , Kim Jin Young , Han Jin Kyu , Kim Jinkyung , Yang Hyejin , Yoon Minseok , Kim Jiyoung , Kang Seung Wan , Cho Suengmok 
Published: January 2018

Our previous study demonstrated that phlorotannin supplement had a sleep‐promoting effect in rodents. In the present study, we investigated whether the phlorotannin supplement could improve sleep in subjects with self‐reported sleep disturbances. In a randomized, double‐blind, placebo‐controlled trial, 24 subjects consumed either a placebo or phlorotannin supplement (500 mg/day) for 1 week, 30–60 min prior to bedtime. Sleep parameters were assessed at baseline and at 1 week with sleep questionnaires and polysomnography. At the end of the treatment period, the complete sets of sleep parameters from 20 subjects. Phlorotannin resulted in a significant increase in “Sleep duration” scores compared to the placebo (p = .044), although there were no significant differences on the total PSQI scores. Polysomnography revealed that wakefulness after sleep onset was significantly lower in the phlorotannin group compared to the placebo group (phlorotannin vs. placebo, −25.5 ± 30.5 vs. −1.7 ± 14.9; p = .045) as well as total wake time (phlorotannin vs. placebo, −0.9 ± 3.0 vs. −6.1 ± 6.8; p = .048). Additionally, the respiratory disturbance index during supine rapid eye movement sleep was significantly lower in the phlorotannin group (p = .035). There were no serious adverse effects in either group. Our data suggest that the phlorotannin supplement improved sleep maintenance (WHO ICTRP: KCT0001892).
Barbigerone reverses multidrug resistance in breast MCF‐7/ADR cells
Authors: Li Xiuxia , Wan Li , Wang Fang , Pei Heying , Zheng Li , Wu Wenshuang , Ye Haoyu , Wang Yanping , Chen Lijuan 
Published: January 2018

Development of agents to overcome multidrug resistance (MDR) is one of the important strategies in cancer chemotherapy, and P‐glycoprotein (P‐gp) correlates with the degree of resistance. As a naturally occurring isoflavone, whether barbigerone (BA) could reverse MDR, is unknown. In this paper, we evaluated effects of BA on reversing P‐gp mediated MDR of adriamycin (ADR)‐resistant human breast carcinoma (MCF‐7/ADR) cells. BA (0.5 μM) treatment showed strong potency to increase ADR cytotoxicity toward MCF‐7/ADR cells. It was also demonstrated that BA time‐ and dose‐dependently increased accumulations of ADR and reduced the efflux in MCF‐7/ADR cells, pretreatment of these cells with BA might relocalized ADR to the nuclei. Furthermore, the results also revealed that BA did not affect P‐gp, but alter P‐gp ATPase activity. Intravenous administration of BA significantly increased anticancer efficacy of ADR to MCF‐7/ADR xenograft model in nude mice. These results revealed that BA might reverse P‐gp mediated MDR through inhibition of ATPase activity, which indicated a novel use of BA as a potent candidate for cancer chemotherapy.
Negative nucleotide ions as sensitive probes for energy specificity in collision‐induced fragmentation in mass spectrometry
Authors: Ickert Stefanie , Riedel Jens , Beck Sebastian , Linscheid Michael W. 
Published: January 2018

RationaleThe most commonly used fragmentation methods in tandem mass spectrometry (MS/MS) are collision‐induced dissociation (CID) and higher energy collisional dissociation (HCD). While in CID the preselected ions in the trap are resonantly (and m/z exclusively) excited, in HCD the entire m/z range experiences the dissociative acceleration. The different excitation is reflected in different fragment distributions.MethodsAs a test‐bed for particularly pronounced fragmentation specificity, here MS/MS experiments on several 4‐mer oligonucleotides were conducted employing both collision methods and the results were thoroughly compared. Oligonucleotides are shown to be sensitive probes to subtle changes, especially in the negative ion mode. A detailed analysis of these differences reveals insight into the dissociation mechanics.ResultsThe differences are represented in heat‐maps, which allow for a direct visual inspection of large amounts of data. In these false colour representations the, sometimes subtle, changes in the individual dissociation product distributions become distinct. Another advantage of these graphic plots can be found in the formation of systematic patterns. These patterns reflect trends in dissociation specificity which allow for the formulation of general rules in fragmentation behavior.ConclusionsInstruments equipped with two different excitation schemes for MS/MS are today widely available. Nonetheless, direct comparisons between the individual results are scarcely made. Such comparative studies bear a powerful analytical potential to elucidate fragmentation reaction mechanism.
Comparing the fragmentation reactions of protonated cyclic indolyl α‐amino esters in quadrupole/orbitrap and quadrupole time‐of‐flight mass spectrometers
Authors: Cao Xiaoji , Cai Xue , Mo Weimin 
Published: January 2018

RationaleThe comparative study of higher‐energy collisional dissociation (HCD) and collision‐induced dissociation (CID) mechanisms for protonated cyclic indolyl α‐amino esters in quadrupole/orbitrap (Q/Orbitrap) and quadrupole time‐of‐flight (QTOF) mass spectrometers, respectively, is helpful to study the structures and properties of biologically active indole derivatives using tandem mass spectrometry (MS/MS) technology.MethodsHCD and CID experiments were carried out using electrospray ionization Q/Orbitrap MS and QTOFMS in positive ion mode, respectively. Only the labile hydrogens were exchanged with deuterium in hydrogen/deuterium exchange (HDX) experiments and only the aromatic indole C‐H hydrogens were substituted with deuterium in regiospecific hydrogen‐deuterium labeling experiments. Theoretical calculations were carried out using the density functional theory (DFT) method at the B3LYP level with the 6‐311G(d,p) basis set in the Gaussian 03 package of programs.ResultsIn Q/Orbitrap MS/MS, when the added proton on the N8 position of protonated cyclic indolyl α‐amino esters migrated in a stepwise fashion to the C3 position via two sequential 1,4‐H shifts, an ion‐neutral complex INC1 of [protonated cyclic N‐sulfonyl ketimino esters/indoles] was formed by a charge‐directed heterolytic cleavage of the C3–C10 bond, while an ion‐neutral complex INC3 of [cyclic N‐sulfonyl ketimino esters/protonated indoles] was formed when another labile hydrogen on the N8 position successively migrated to the C4 position. Direct decomposition of INC1 and INC3 resulted in protonated cyclic N‐sulfonyl ketimino esters and protonated indoles, respectively, while proton transfer led to protonated indoles and protonated cyclic N‐sulfonyl ketimino esters. The HDX reaction with residual water in the HCD cell was also observed. In QTOF‐MS/MS, protonated cyclic N‐sulfonyl ketimino esters and protonated indoles resulted from direct decomposition of INC1 and INC3, respectively, rather than proton transfer.ConclusionsDue to the specific construction of the Q/Orbitrap and QTOF mass spectrometers, different fragmentation mechanisms medicated by ion‐neutral complexes of protonated cyclic indolyl α‐amino esters were proposed. This study is desirable for qualitative and quantitive investigation of indole derivatives using MS/MS technology.