bluuebunny/crossref_metadata_2025_split huggingface dataset:
S-shaped Wound Closure Technique for Dumbbell-shaped Keloids
Authors: Komatsu Seiji , Azumi Shougo , Hayashi Yuko , Morito Tsuneharu , Kimata Yoshihiro 
Published: March 2017

Summary:
Dog-ear collection, Z-plasty, and W-plasty are often performed for excision of dumbbell-shaped keloids; however, these procedures require additional incisions or excision of normal skin. Thus, an S-shaped wound closure technique was performed. The keloid lesions were extralesionally excised above the deep fascia, and the wound edges were shifted in opposite directions along the major axis to form an S-shape. The incision was closed by applying deep fascial sutures, subcutaneous sutures, and superficial sutures. Postoperative external beam radiation therapy was started within 6 hours after surgery at a dose of 20 Gy applied in 4 fractions. All wounds were covered with silicone-gel sheeting and fixed with tape after suture removal. No intralesional corticosteroid injection or oral tranilast was administered. Corticosteroid tape was applied in cases with suspected postoperative recurrence. Scoring was performed using the Manchester Scar Scale. A total of 8 lesions were treated. Temporary erythema and scar elevation were observed in 2 chest lesions; however, both were flattened and turned white using corticosteroid tape. Other than these 2 lesions, there was no recurrence or complication. The mean score improved from 15.8 to 7.2. The S-shaped wound closure technique has 3 advantages. First, no additional incision or excision is required, and additional scarring and keloid recurrence can be avoided. Second, aesthetic results are good, and noticeably long and zigzag-shaped scars can be avoided. Third, dispersion of tension on the scar can be expected. Although the S-shaped wound closure technique has limited application, it is a useful option for keloid treatment.
STENOTROPHOMONAS MALTOPHILIA ENDOPHTHALMITIS 1 MONTH AFTER INTRAVITREAL AFLIBERCEPT
Authors: Boeke Paul S. , Gottlieb Justin L. 
Published: March 2017

Purpose:
Endophthalmitis caused by Stenotrophomonas maltophilia is rare and has been described after cataract surgery and open globe injuries. We report a patient with endophthalmitis caused by this organism after uncomplicated intravitreal aflibercept injection.

Methods:
A 70-year-old man with a history of anti–vascular endothelial growth factor therapy for diabetic macular edema presented 23 days after aflibercept injection with clinical diagnosis of endophthalmitis. The patient initially had mild pain, conjunctival congestion, and anterior chamber cell; 2 days later, the patient returned with hand motion visual acuity, hypopyon, and dense vitritis.

Results:
A tap and inject procedure was performed. The aqueous sample confirmed the presence of S. maltophilia. Antimicrobial testing showed susceptibility to ceftazidime which was used during the initial treatment. After resolution of the vitritis and hypopyon, the visual acuity returned to 20/70 at his 3-month follow-up examination.

Conclusion:

Stenotrophomonas maltophilia is a rare infectious agent associated with intravitreal injection and may present 1 month after treatment.
Transmission of Donor-Derived Breast Carcinoma as a Recurrent Mass in a Keratolimbal Allograft
Authors: Miller Audra K. , Young Jonathan W. , Wilson David J. , Dunlap Jennifer , Chamberlain Winston 
Published: March 2017

Purpose:
To report a case of local transmission of invasive lobular carcinoma from a donor to a recipient in a keratolimbal allograft after cessation of systemic immunosuppressive therapy.

Methods:
This is a case report including the clinicopathologic findings. Sections of the donor breast tumor and recipient conjunctival lesions were stained with hematoxylin and eosin. Immunohistochemical studies were performed using pancytokeratin, CK7, CK20, CAM 5.2, CD138, TTF1, estrogen receptor, progesterone receptor, GATA-3, GCDFP-15, and mammaglobin. Polymerase chain reaction-based DNA profiling of tumor cells was performed.

Results:
Histopathologic examination revealed an infiltrate of atypical cells with large hyperchromatic nuclei consistent with carcinoma. Immunohistochemical analysis showed pancytokeratin, CK7, CAM 5.2, GATA-3, and estrogen receptor positivity and progesterone receptor absence, consistent with the previously determined phenotype of the donor's breast carcinoma. Results of polymerase chain reaction analysis were also consistent with the donor's tumor. After reduced dosing of tacrolimus and mycophenolate mofetil, 2 limbal tumors occurred in the recipient. The immunosuppressive treatment had been stopped completely before the appearance of the third lesion. The recipient had no history of malignancy, and she had routine screenings for breast cancer.

Conclusions:
We report a case of donor-derived breast carcinoma in a keratolimbal allograft recipient. The grafted tissue harbored donor-derived tumor cells for more than 4 years after surgery even after systemic immunosuppression was discontinued. Although no similar reports of tumor transfer could be found in the literature, this case suggests the need for increased stringency in donor selection and heightened surveillance for such tumor transmission.
Measuring Forward Light Scatter by the Boston Keratoprosthesis in Various Configurations
Authors: Abdelaziz Musa , Dohlman Claes H. , Sayegh Rony R. 
Published: March 2017

Purpose:
Light scatter results in degradation of visual function. An optical bench model was used to identify the origins of scatter in the setting of a Boston keratoprosthesis (KPro). The effect of various modifications in the device design and light-blocking configurations was explored.

Methods:
A KPro was mounted on a contact lens holder on a bench, and forward light scatter was recorded with a camera attached to a rotating goniometer arm. Scattered light was recorded at different angles for different KPro modifications, and the point-spread function (PSF) curves were recorded. The area under the curve (AUC) was calculated for each PSF curve.

Results:
The isolated KPro optical cylinder in a totally blackened holding lens had a tight PSF (AUC = 3.3). Additional blackening of the walls of the KPro stem did not further diminish forward scatter significantly. If the holding lens is made translucent by sandblasting (to simulate an in vivo carrier cornea) and the KPro is inserted without a backplate, forward scatter is substantial (AUC = 11.3). If a standard backplate (with holes) is added, light scatter is considerably reduced regardless of whether the backplate is made of polymethyl methacrylate or titanium (AUC = 5.3 and 4.4, respectively). Addition of an acrylic intraocular lens behind the KPro (the pseudophakic KPro setup) did not increase scatter.

Conclusions:
Most of the scattered light in eyes implanted with a KPro originates from the surrounding hazy corneal graft. The standard addition of a backplate reduces light scatter. There was no difference in forward light scatter between the aphakic and the pseudophakic KPro.
Presurgical Concerns of Primary Family Caregivers of Children With Cerebral Palsy
Authors: Yu Li-Chun , Chen Li-Chin , Lin Hsiu-Chen , Lin Yueh-E. , Chang Chia-Hsieh , Chen Shu-Ching 
Published: March 2017

BACKGROUND:
Primary family caregivers (PFCs) of children with cerebral palsy have many worries and concerns when their children face orthopedic surgery. Levels of PFC stress about the upcoming surgery is related to the child's level of gross motor function as well as the support they receive from medical professionals.

PURPOSE:
The purposes of the present study were to (1) explore the levels of concern about orthopedic surgery; and (2) explore the predictive factors associated with concerns about orthopedic surgery among PFCs of children with cerebral palsy during the preoperative period.

METHODS:
A cross-sectional, correlational study was conducted. Primary family caregivers were assessed preoperatively using the Single-event Multilevel Surgery Scale, Social Support Scale, Gross Motor Function Classification System–Expanded and Revised, and background information form. Primary family caregivers were recruited from the outpatient department of orthopedic surgery and pediatric rehabilitation of a medical center in northern Taiwan. Data were analyzed by descriptive analysis, Pearson product–moment correlation, and multiple regression analysis.

RESULTS:
A total of 63 eligible subjects were enrolled in this study. Primary family caregivers had moderate levels of concern and mild-to-moderate levels of social support. The higher severity of motor function impairment in children with cerebral palsy, prior caregiving by PFCs for another family member, and PFCs' lower level of social support from healthcare providers were associated with higher levels of PFC concern.

CONCLUSIONS:
Concerns about orthopedic surgery is an overlooked issue that needs more attention from healthcare providers. This study determined that PFCs who perceived a lack of social support from their healthcare providers and those with children who had limited gross motor function were more concerned and anxious about their children's upcoming orthopedic surgery. Health professionals should provide adequate health education and counseling to help PFCs of children with cerebral palsy in the decision-making process prior to orthopedic surgery.
Urine glucose levels are disordered before blood glucose levels increase in Zucker diabetic fatty rats
Authors: Yin Wei , Qin Weiwei , Gao Youhe 
Published: March 2017

AbstractDiabetes mellitus is a type of metabolic disease marked by hyperglycemia, and 90% of diabetes cases are type 2 diabetes mellitus. More than half of patients are not diagnosed at all or are diagnosed too late to be effectively treated, resulting in nonspecific symptoms and a long period of incubation of the disease. Pre-diabetes mellitus, also known as impaired glucose regulation, is an early warning signal of diabetes, which has long been determinated by impaired fasting glucose and impaired glucose tolerance. In this study, Zucker diabetic fatty (ZDF) rats were used to test if there were changes in urine glucose before blood glucose increases. Six 8-week-old male ZDF rats (fa/fa) and Zucker lean (ZL) rats (fa/+) were fed with Purina 5008 high-fat diet and tested for fasting blood glucose and urine glucose. After 12 weeks of feeding, the urine glucose values of the ZL rats were normal (0-10 mmol/L), but the values of the ZDF model rats increased 10 weeks before their blood glucose levels elevated. The urine glucose values of the ZDF model rats showed a state of disorder that was frequently elevated (>10 mmol/L) and occasionally normal (0-10 mmol/L). This finding may provide an easy early diagnosis. Screening for human diabetes can be considered by frequently monitoring urine glucose levels: pre-diabetes may be revealed by frequently disordered urine glucose levels over a period.
xMWAS: an R package for data-driven integration and differential network analysis
Authors: Uppal Karan , Go Young-Mi , Jones Dean P. 
Published: March 2017

AbstractSummaryIntegrative omics is a central component of most systems biology studies. Computational methods are required for extracting meaningful relationships across different omics layers. Various tools have been developed to facilitate integration of paired heterogenous omics data; however most existing tools allow integration of only two omics datasets. Further-more, existing data integration tools do not incorporate additional steps of identifying sub-networks or communities of highly connected entities and evaluating the topology of the integrative network under different conditions. Here we present xMWAS, an R package for data integration, network visualization, clustering, differential network analysis of data from biochemical and phenotypic assays, and two or more omics platforms.Availabilityhttps://sourceforge.net/projects/xmwas/Contactkuppal2@emory.edu
Agreement between measurements of stance width using motion capture and center of pressure in individuals with and without Parkinson’s disease
Authors: McKay J. Lucas 
Published: March 2017

AbstractBackgroundMany individuals with Parkinson’s disease exhibit narrow stance width during balance and gait. Because of this, stance width is an important biomechanical variable in many studies. Measuring stance width accurately using kinematic markers in parkinsonian patients can be problematic due to occlusions by research staff who must closely guard patients to prevent falls.MethodsWe investigated whether a measure of stance width based on the mediolateral distance between the center of pressure under each foot could approximate stance width measured with kinematic data. We assessed the agreement between estimates of stance width obtained from simultaneous kinematic and center of pressure measures during quiet standing in 15 individuals (n=9 parkinsonian, n=6 age-similar neurotypical). The source data (1363 unique trials) contained observations of stance width varying between 75–384 mm (≈25-150% of hip width).FindingsStance width estimates using the two measures were strongly correlated (r= 0.98). Center of pressure estimates of stance width were 48 mm wider on average than kinematic measures, and did not vary across study groups (F2,12=1.81,P<0.21). The expected range of differences between the center of pressure and kinematic methods was 14–83 mm. Agreement increased as stance width increased (P<0.02).InterpretationIt is appropriate to define stance width based on center of pressure when it is convenient to do so in studies of individuals with and without Parkinson’s disease. When comparing results across studies with the two methodologies, it is reasonable to assume a bias of 48 mm.
The role of N-terminal modification of MeCP2 in the pathophysiology of Rett syndrome
Authors: Sheikh Taimoor I. , de Paz Alexia Martínez , Akhtar Shamim , Ausió Juan , Vincent John B. 
Published: March 2017

AbstractMethyl CpG-binding protein 2 (MeCP2), the mutated protein in Rett syndrome (RTT), is a crucial chromatin-modifying and gene-regulatory protein that has two main isoforms (MeCP2_E1 and MeCP2_ E2) due to the alternative splicing and switching between translation start codons in exons one and two. Functionally, these two isoforms appear to be virtually identical; however, evidence suggests that only MeCP2_E1 is relevant to RTT, including a single RTT missense mutation in exon 1, p.Ala2Val. Here, we show that N-terminal co- and post- translational modifications differ for MeCP2_E1, MeCP2_E1-p.Ala2Val and MeCP2_E2, which result in different protein degradation ratesin vitro. We report partial N-methionine excision (NME) for MeCP2_E2, whereas NME for MeCP2_E1 is complete. Surprisingly, we also observed evidence of excision of multiple alanine residues from the N-terminal polyalanine stretch. Regarding MeCP2_E1-Ala2Val, we also observed only partial NME and N-acetylation (NA) of either methionine or valine. The localization of MeCP2_E1 and co-localization with chromatin appear to be unaffected by the p.Ala2Val mutation. However, a higher proteasomal degradation rate was observed for MeCP2_E1-Ala2Val compared with that for wild type (WT) MeCP2_E1. Thus, the etiopathology of p.Ala2Val is likely due to a reduced bio-availability of MeCP2 because of the faster degradation rate of the unmodified defective protein. MeCP2_E1 is thought to have a much higher translational efficiency than MeCP2_E2. Our data suggest that this increased efficiency may be balanced by a higher degradation rate. The higher turnover rate of the MeCP2_E1 protein suggests that it may play a more dynamic role in cells than MeCP2_E2.Significance statementThe Rett syndrome protein, MeCP2, undergoes a number of modifications before becoming functionally active in the body’s cells. Here, we report the presence of N-terminal modifications in both MeCP2 isoforms, MeCP2_E1 and MeCP2_E2, and that the only reported Rett missense mutation in exon 1, p.Ala2Val, disrupts these modifications, decreasing the longevity of the protein. Interestingly, p.Ala2Val mutations have been reported in many other disease genes, such asDKCX, ECHS1, IRF6, SMN1, andTNNI3, and the etiopathological mechanism(s) have never been explained. Thus, this work is important not only for the understanding of the pathophysiology of Rett syndrome but also for a deeper understanding of the effects of genetic mutations at the N-terminal end of genes in general.
Implementation and System Identification of a Phosphorylation-Based Insulator in a Cell-Free Transcription-Translation System
Authors: Guo Shaobin , Yeung Enoch , Murray Richard M. 
Published: March 2017

AbstractAn outstanding challenge in the design of synthetic biocircuits is the development of a robust and efficient strategy for interconnecting functional modules. Recent work demonstrated that a phosphorylation-based insulator (PBI) implementing a dual strategy of high gain and strong negative feedback can be used as a device to attenuate retroactivity. This paper describes the implementation of such a biological circuit in a cell-free transcription-translation system and the structural identifiability of the PBI in the system. We first show that the retroactivity also exists in the cell-free system by testing a simple negative regulation circuit. Then we demonstrate that the PBI circuit helps attenuate the retroactivity significantly compared to the control. We consider a complex model that provides an intricate description of all chemical reactions and leveraging specific physiologically plausible assumptions. We derive a rigorous simplified model that captures the output dynamics of the PBI. We perform standard system identification analysis and determine that the model is globally identifiable with respect to three critical parameters. These three parameters are identifiable under specific experimental conditions and we perform these experiments to estimate the parameters. Our experimental results suggest that the functional form of our simplified model is sufficient to describe the reporter dynamics and enable parameter estimation. In general, this research illustrates the utility of the cell-free expression system as an alternate platform for biocircuit implementation and system identification and it can provide helpful insights into future biological circuit designs.
Significance of family-friendly measures on fertility in Hong Kong
Authors: Lam Gigi , Yan Yuruo , Tu Edward Jow-Ching 
Published: March 2017

Purpose
Hong Kong entered an ultra-low fertility regime nearly two decades ago (Census and Statistics Department, 2013). The causes of ultra-low fertility in Hong Kong are the same as those in other developed economies (Tu and Lam, 2009). The phenomenon, in most of the western world and East Asian societies, is attributed to the incongruence between individual-oriented and family-oriented institutions (McDonald, 2000), or simply role incompatibility between work and motherhood (Stycos and Weller, 1967). One viable solution to alleviate role incompatibility is to introduce family-work reconciliation policies, including maternal and paternal leaves, subsidized child care and health care and work facilities that allow for breastfeeding (Lappegard, 2010). The purpose of this paper is to assess the family-friendly measures for enhancing fertility.

Design/methodology/approach
The paper analyzes the current demographic conundrum and makes recommendations.

Findings
Subsidized child care is an effective measure if it satisfies the five main criteria, namely, availability, accessibility, acceptability, cost, and quality, suggested by Rindfuss et al. (2003). Other family-friendly measures are inadequate in absolute terms and inferior to those of Asian countries such as Japan, Singapore, and South Korea (Ministry of Manpower, 2014; OECD, 2013). The possibility of shifting away from the ultra-low fertility regime remains doubtful, especially because low fertility is a combined effect of an increasing prevalence of single older women (Census and Statistics Department, 2014), a shift of the utility function of children toward other consumable goods (Inglehart, 1982) and a desire for achieving upward intragenerational and intergenerational social mobility (Ariès, 1980).

Practical implications
Since Hong Kong still subsides in the regime of the lowest-low fertility, an evaluation of the related family-friendly measures will provide constructive insights to the Hong Kong Special Administrative Region Government how to provide incentives to citizens to consider making childbearing decisions.

Originality/value
Because the introduction of family-friendly measures and gender ideologies are intractably linked (Brewster and Rindfuss, 2000), Hong Kong stays in the middle of nations of families and nations of individuals, influenced by western ideas and traditional family values. It is hence worthwhile to examine the effectiveness of different family-friendly measures.
A unique pattern of one-child family migration in China
Authors: Mu Yingtan , Yuan Xin 
Published: March 2017

Purpose
At the end of the 1970s, the Chinese government enacted the one-child policy; now the one-child successively enters into the labor market and reaches the age for marriage and childbirth. The floating population group of China’s interior regions also experiences the heterogeneity changes. The purpose of this paper is to analyses the reasons for the difference of family migration between one-child and non-only child using the binary logit regression model – from the three aspects of individual characteristics, family endowment and institutional factors were investigated.

Design/methodology/approach
Family migration or individual migration of the floating population is the dichotomous dependent variable and therefore the binomial logistic regression analysis model is selected.

Findings
It is found that the tendency of one-child family migration is significantly higher than that of non-only child. The main reason is that the one-child has obvious advantages in terms of individual characteristics, family endowment and institutional factors.

Originality/value
The previous researches on family migration: first, the previous researches mainly analyzed the impact of the human capital and family income on the family migration from the perspective of economics and neglected the discussion on the family structure, life cycle, family level factors and Hukou’s limitation; second, most researches considered the migration as a whole. In fact, the migration population is no longer a highly homogeneous group and gradually become diversified.