bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal observational study of hidradenitis suppurativa: impact of surgical intervention with adjunctive biologic therapy
Authors: Shanmugam Victoria K. , Mulani Shaunak , McNish Sean , Harris Sarah , Buescher Teresa , Amdur Richard 
Published: November 2017

AbstractBackgroundHidradenitis supppurativa (HS) is a chronic inflammatory disease of the apocrine sweat glands affecting 1–4% of the population. While surgical excision is a mainstay of therapy, lesions often recur. Biologic therapies, including tumor necrosis factor‐α and IL‐12/23 inhibitors, are effective for mild to moderate HS. However, longitudinal studies investigating biologic therapy in conjunction with surgery are limited. The purpose of this analysis was to investigate impact of surgery and biologic therapy on HS disease activity.MethodsData from 68 HS patients were analyzed. Outcome measures included hidradenitis suppurativa Sartorius Score (HSS), active nodule (AN) count, Hurley stage, and probability of achieving 75% reduction in active nodule count (AN75).ResultsMean age was 40 ± 14 years; 66% were female and 72% were African American. Mean disease duration was 10 years, and Hurley stage III disease was seen in 63% of patients. Patients who received biologics had a larger drop in HSS and AN count than those who never received biologics (P = 0.002). Biologic treatment was associated with average reduction in 22 (15–29) HSS points (P < 0.0001). The effect of biologics was greater in patients who also underwent surgery (P = 0.013). Timing of biologics relative to surgery did not impact efficacy. Patients who received HS surgery with biologic therapy were most likely to achieve the AN75 (P = 0.017).ConclusionsIn this diverse cohort of patients with severe HS, biologic therapy was associated with a more rapid decline in disease activity, with the greatest effect in patients who also underwent HS surgery.
Pellicle formation, microbial succession and lactic acid utilisation during the aerobic deteriorating process of Sichuan pickle
Authors: Rao Yu , Qian Yang , She Xiao , Yang Jiantao , He Penghui , Jiang Yunlu , Wang Meng , Xiang Wenliang 
Published: November 2017

AbstractThe pellicles formation, microbial changes and lactic acid utilisation during the aerobic deterioration of Sichuan pickle were discussed in this study. By plate counting, chemical detection and HPLC analysis, the aerobic deterioration of Sichuan pickle was featured by microbial growth, pellicle formation, lactic acid decrease and pH elevation. Bacteria dominated in pellicles and the pellicles present morphological change from smooth to wrinkly along with the aerobic deteriorating process. Nine species of bacteria were identified and responsible for the pellicle formation. Combined with Lactobacillus plantarum, eight pellicle‐forming bacteria were the dominant lactic acid consuming organisms in deteriorating Sichuan pickle. Especially under the harsh acid condition of the early deteriorating period, Lb. plantarum, pellicle‐forming bacteria Bacillus amyloliquefaciens, B. subtilis and Citrobacter freundii participated in the pellicles, utilised lactic acid and increased the brine pH effectively. Sequence analysis of 16S rDNA libraries suggested that the resulting pH of 4.8–5.0 initiated the growth of more undesirable organisms, and the pellicle bacterial diversity changed greatly compared with that in the early deteriorating period. The study indicated the important role of pellicles in the initiation of Sichuan pickle's aerobic deterioration and enhanced our ability to understand and potentially control the aerobic deterioration.
Changes in degradation and phosphorylation level of titin in three ovine muscles during postmortem
Authors: Wang Ying , Li Xin , Li Zheng , Li Meng , Zhu Jie , Zhang Dequan 
Published: November 2017

SummaryThe objective of this study was to investigate the degradation of titin and its phosphorylation level in three muscles from sheep. The MFI and pH from the longissimus lumborum (LL), semimembranosus (SM) and psoas major (PM) muscles were measured at 30 min, 1, 2, 7, 14, 21 and 28 days postmortem. Myofibrillar proteins were extracted, separated by SDS‐PAGE and quantified by phosphor‐specific staining. Phosphorylation of titin was predicted by Pro‐Q Diamond‐SYPRO Ruby staining. Two days after exsanguination, the pH and MFI of the PM were higher than those of the LL and SM muscles (P < 0.05). The sarcomere length of the PM muscle was also longer than that of the LL and SM muscle (P < 0.05). PM muscles had a highest phosphorylation level (P < 0.05) at 0.5 h postmortem and showed the greatest degree of titin degradation over 28 days. This suggests that phosphorylation of titin might accelerate its degradation.
Influence of composition on degradation during repeated deep‐fat frying of binary and ternary blends of palm, sunflower and soybean oils with health‐optimised saturated‐to‐unsaturated fatty acid ratios
Authors: Rudzińska Magdalena , Hassanein Minar M. M. , Abdel‐Razek Adel G. , Kmiecik Dominik , Siger Aleksander , Ratusz Katarzyna 
Published: November 2017

SummaryThe American Heart Association recommended the fatty acid balance contributed by all the fats in our diet, suggesting it would be best at approximately 1:1:1 for saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA), respectively. Three individual oils: palm oil (PO), soybean oil (SBO) and sunflower oil (SNF) and their binary and ternary blends were prepared and used for repeated deep‐fat frying of French fries. The acid value, peroxide value, p‐anisidine value and Totox values, as well as oxidative stability, contents of total polar compounds, tocochromanols, triacylglycerol dimers and oligomers, were determined in individual and blended oils. The lowest Totox value and highest stability were found for PO, and the opposite data were obtained for SNF and SBO. The degradation of tocochromanols in blends ranged from 91% to 95% after 4 days of frying, while in individual oils, it was 63% in SBO, 71% in SNF and 100% in PO. The lowest formation of dimers and oligomers was observed for the PO: SNF blend. Obtained results showed that only pure PO was a better frying medium than its blends with SBO and SNF. However, a prepared blend had a better fatty acid composition for human health and was more stable than pure SBO and SNF.
Literature review of the epidemiology of influenza B disease in 15 countries in the Asia‐Pacific region
Authors: Jennings Lance , Huang Qiu Sue , Barr Ian , Lee Ping‐Ing , Kim Woo Joo , Buchy Philippe , Sanicas Melvin , Mungall Bruce A. , Chen Jing 
Published: November 2017

Influenza control strategies focus on the use of trivalent influenza vaccines containing two influenza A virus subtypes and one of the two circulating influenza type B lineages (Yamagata or Victoria). Mismatches between the vaccine B lineage and the circulating lineage have been regularly documented in many countries, including those in the Asia‐Pacific region. We conducted a literature review with the aim of understanding the relative circulation of influenza B viruses in Asia‐Pacific countries. PubMed and Western Pacific Region Index Medicus were searched for relevant articles on influenza type B published since 1990 in English language for 15 Asia‐Pacific countries. Gray literature was also accessed. From 4834 articles identified, 121 full‐text articles were analyzed. Influenza was reported as an important cause of morbidity in the Asia‐Pacific region, affecting all age groups. In all 15 countries, influenza B was identified and associated with between 0% and 92% of laboratory‐confirmed influenza cases in any one season/year. Influenza type B appeared to cause more illness in children aged between 1 and 10 years than in other age groups. Epidemiological data for the two circulating influenza type B lineages remain limited in several countries in the Asia‐Pacific, although the co‐circulation of both lineages was seen in countries where strain surveillance data were available. Mismatches between circulating B lineages and vaccine strains were observed in all countries with available data. The data suggest that a shift from trivalent to quadrivalent seasonal influenza vaccines could provide additional benefits by providing broader protection.
Comparative phylogeography of two co‐distributed but ecologically distinct rainbowfishes of far‐northern Australia
Authors: Mather Andrew T. , Hanson Jeffrey O. , Pope Lisa C. , Riginos Cynthia 
Published: November 2017

AbstractAimTo test the influence of historical and contemporary environment in shaping the genetic diversity of freshwater fauna we contrast genetic structure in two co‐distributed, but ecologically distinct, rainbowfish; a habitat generalist (Melanotaenia splendida) and a habitat specialist (M. trifasciata).LocationFishes were sampled from far northern Australia (Queensland and Northern Territory).MethodsWe used sequence data from one mitochondrial gene and one nuclear gene to investigate patterns of genetic diversity in M. splendida and M. trifasciata to determine how differences in habitat preference and historical changes in drainage boundaries affected patterns of connectivity.ResultsMelanotaenia splendida showed high levels of genetic diversity and little population structure across its range. In contrast, M. trifasciata showed high levels of population structure. Whereas phylogeographic patterns differed, both species showed a strong relationship between geographical distance and genetic differentiation between populations. Melanotaenia splendida showed a shallower relationship with geographical distance, and genetic differentiation was best explained by stream length and a lower scaled ocean distance (11.98 times coast length). For M. trifasciata, genetic differentiation was best explained by overwater distance between catchments and ocean distance scaled at 1.16 × 106 times coast length.Main conclusionsConnectivity of freshwater populations inhabiting regions periodically interconnected during glacial periods appears to have been affected by ecological differences between species. Species‐specific differences are epitomized here by the contrast between co‐distributed congeners with different habitat requirements: for the habitat generalist, M. splendida, there was evidence for greater historical genetic connectivity with oceans as a weaker barrier to gene exchange in contrast with the habitat specialist, M. trifasciata.
In‐office bleaching efficacy on stain removal from CAD/CAM and direct resin composite materials
Authors: Alharbi Amal , Ardu Stefano , Bortolotto Tissiana , Krejci Ivo 
Published: November 2017

AbstractObjectiveTo evaluate the efficacy of in‐office bleaching on stain removal from stained resin composite and ceramic computer‐assisted design/computer‐assisted manufacturing (CAD/CAM) blocks and direct resin composites.MethodsForty disk‐shaped samples were fabricated from each of nine materials: six CAD/CAM (VITABLOCS Mark II, Paradigm MZ100, Exp Vita Hybrid Ceramic, VITA ENAMIC, Exp Kerr, and LAVA Ultimate) and three direct resin composites (Filtek Supreme, Venus Diamond, and Filtek Silorane). Samples were randomly divided into five groups (n = 8), each stained with a particular staining solution. Using a calibrated spectrophotometer and a black background, L*a*b* values were assessed before and after 120 days of staining. Samples were subjected to in‐office bleaching using 40% hydrogen peroxide gel for one hour. At subsequent assessment, color change (ΔE) was calculated as the difference between L*a*b* values. Both ANOVA and the Duncan test were used to identify differences between groups (α = 0.05).ResultsBleaching resulted in significant differences in ΔE values for all materials (P < .001). Bleaching efficacy was highly influenced by material composition and staining solution. Residual color values after bleaching for ceramic and hybrid ceramics ranged from −0.49 to 2.35, within the clinically acceptable maximum of 3.3. Values after bleaching for resin‐based CAD/CAM ranged from −0.7 to 7.08 while direct resin composites values ranged from −1.47 to 25.13. Coffee left the greatest residual color on all materials.ConclusionsBased on material nature, 40% hydrogen peroxide bleaching can remove staining. The new resin‐based CAD/CAM blocks showed promising results in terms of color stability.Clinical SignificanceBleaching using 40% hydrogen peroxide can be an effective method to remove stains from dental restorations. In this way, restoration replacement as a result of discoloration may no longer be necessary.
Efficacy of subcutaneous injection of platelet‐rich plasma in alopecia: A clinical and histological pilot study on a rat model with a six‐month long‐term follow‐up experience
Authors: Orliac Sophie , Serfaty Jean‐Michel , Perozziello Anne , Zurlinden Olivier , Louedec Liliane , Dallaudière Benjamin 
Published: November 2017

SummaryPurposeTo assess the potential of platelet‐rich plasma (PRP) subcutaneous injection of to treat alopecia and to evaluate local toxicity.Materials‐MethodsTwelve Hairless rats were used. At D0, we performed systematic clinical examination and divided the rat back into four quadrants (Q). We initiated subcutaneous injection using either PRP in PRPQ+, platelet‐poor plasma (PPP) in PPPQ+, physiological serum (PS) in PSQ+, or no treatment (Q4). At D7, D14, D21, and D28 but also second month (M2), M3, M4, M5, rats had exactly the same injection procedure. Follow‐up with PRP efficacy and toxicity at D28 and M6 using clinical and histological evaluation was performed.ResultsHair density was significantly improved at D28 and at M6 for PRPQ+ vs PSQ+ (respectively, P = .0156 and P = .0313), PPPQ+ (respectively, P = .042 and P = .046). Significant histological improvement was observed between D28 and M6, for PRPQ+ vs PPPQ+ and PSQ+ for vessels (respectively, P = .0160 and P = .021), collagen (respectively, P = .0036 and P = .032), and epithelium (respectively, P = .0138 and P = .022) with no local toxicity.ConclusionOur study suggests that subcutaneous PRP injections using controlled concentration of platelets and leukocytes improve hair growth.
Filling the periorbital hollows with hyaluronic acid gel: Long‐term review of outcomes and complications
Authors: Mustak Hamzah , Fiaschetti Danica , Goldberg Robert Alan 
Published: November 2017

SummaryIntroductionHyaluronic acid gel has been used for rejuvenation of the periorbital tissues since 2004. The in‐office, nonsurgical procedural nature has resulted in the growing popularity of the use of fillers. The periorbital region poses unique challenges to the cosmetic surgeon. Malar edema, blue‐gray dyschromia, and contour irregularities are well reported short‐term complications. We present a long‐term follow‐up review of complications associated with periocular injection of hyaluronic acid gel fillers.MethodsRetrospective review of 147 patients with at least a 5‐year follow‐up period since the first injection. Five senior masked reviewers were asked to grade photographs of patients in comparison with preinjection photographs. Grading was based on a reference photographic key looking specifically at the presence and severity of malar edema, blue‐gray dyschromia, and contour irregularity.ResultsMalar edema (11%), blue‐gray dyschromia (31.3%), and contour irregularities (30.5%) continue to be problems in longer term follow‐up of patients. The vast majority (90%) of these complications were mild and required no intervention. There is no statistically significant difference in the mean volumes injected by severity grading of each measured complication.ConclusionHyaluronic acid gel fillers of the periorbital region are well tolerated in our long‐term follow‐up experience. The vast majority of cases of malar edema, blue‐gray dyschromia, and contour irregularities are mild and do not require intervention. Conservative treatment and tailored treatment approaches, in terms of volume and frequency of injections, may decrease the tendency of these adverse effects to occur.
Oleozon: A novel control strategy against powdery mildew in cucumber
Authors: Ma Yu , Ke Yang , Zhu Haiyun , Li Bo , Li Baoqing , Zhang Feng , Li Yingmei 
Published: November 2017

AbstractOzonized sunflower oil (oleozon) is an effective agent for controlling powdery mildew in cucumber. In this study, the mechanisms of oleozon in the control of powdery mildew were determined. The development of Podosphaera xanthii on cucumber leaves treated with oleozon (2%) and water was investigated at different times after inoculation. The germinating conidia, hyphae and conidiophores of the pathogen were severely damaged by oleozon. No visible phytotoxic effect was observed on cucumber after the application of oleozon. This compound had highly preventive effects as well as curative effects against powdery mildew based on in vivo potted seedling assays. The control effects of oleozon were further confirmed in a greenhouse trial. These results may provide a basis for further development of a natural fungicide against cucumber powdery mildew.
Molecular identification and characterization of Elsinoë murrayae (Synonym: Sphaceloma murrayae) from weeping willow
Authors: Zhao Lijuan , Zhang Weitao , Xiao Hongju , Xiong Qin , Cheng Qiang 
Published: November 2017

AbstractSphaceloma murrayae is a significant fungal pathogen of Salix spp. It causes greyish‐white leaf spots, which were reported worldwide except in China. Its morphological characteristics were described in the early literature; however, there is a lack of molecular information pertaining to this fungus. This study identified and characterized three fungal isolates that obtained from weeping willow leaf spots in China. Based on disease symptoms, morphological characteristics and single nomenclature rules for fungi, these isolates are proposed to be new combinations of Elsinoë murrayae (Synonym: S. murrayae). Phylogenetic analysis that combined internal transcribed spacer (ITS), large subunit (LSU), RBP2 and TEF1‐α DNA sequences indicated that E. murrayae isolates and Elsinoë salicina—another Elsinoë sp. isolated from Salix sp.—were distinguishable species. With trypan blue staining and stereomicroscopic observation, we found that large‐scale cell death occurred at 2 days postinoculation (dpi) and slight disease symptoms started at 3 dpi when the conidia were inoculated on Salix babylonica leaves. Pathogenicity analysis revealed that three isolates can successfully infect mature leaves of S. babylonica, Salix fragilis and Salix suchowensis, but not Salix matsudana. In addition, a necrosis‐ and ethylene‐inducing‐like proteins’ (NLP) gene, named EmNLP1, was cloned. The cytotoxicity of EmNLP1 was confirmed by transient assay in tobacco. During infection, EmNLP1 dramatically peaked at 2 dpi and maintained a high‐level expression in the necrosis lesion growing stage.
Liver mitochondrial oxygen consumption and efficiency of milk production in lactating Holstein cows supplemented with copper, manganese and zinc
Authors: Acetoze G. , Champagne J. , Ramsey J. J. , Rossow H. A. 
Published: November 2017

SummaryThe objective of this study was to examine the relationship between mitochondrial proton leak and feed efficiency with supplementation of different levels of Cu, Mn and Zn (Bioplex, Alltech) at levels above Nutrient Requirements of Dairy Cattle (NRC, 2001). Milk yield and composition, mineral excretion in milk and faeces, feed efficiency and liver mitochondrial proton leak were measured in 60 Holstein dairy cows at approximately 70 days in milk on a commercial dairy. Treatments reflect total Cu, Mn and Zn intake/day and are as follows: (i) High: 444 mg/day Cu, 3492 mg/day Mn, 2234 mg/day Zn; (ii) Med: 436 mg/day Cu, 3002 mg/day Mn, 2047 mg/day Zn; (iii) Low: 420 mg/day Cu, 2764 mg/day Mn, 2186 mg/day Zn; (iv) LowMn: 391 mg/day Cu, 2617 mg/day Mn, 1849 mg/day Zn; and (v) Control: 264 mg/day Cu, 2850 mg/day Mn, 1593 mg/day Zn. Proton leak‐dependent respiration was lowest in Control (p < .10). However, measures of efficiency were greatest in Med and least in High (p < .10). Therefore, measures of efficiency did not reflect efficiency due to low proton leak and there appears to be an upper limit to beneficial supplementation of Cu, Mn and Zn.
Treatment strategies in coronary microvascular dysfunction: A systematic review of interventional studies
Authors: Suhrs Hannah E. , Michelsen Marie M. , Prescott Eva 
Published: November 2017

AbstractCMD has been associated with a wide spectrum of diseases and conditions, and it has proven to be a strong prognostic marker of morbidity and mortality. Despite increased attention, guideline‐based treatment recommendations are lacking. We performed a systematic review of pharmacological and nonpharmacological interventions to improve coronary perfusion, assessed by IC Doppler, TTDE, PET, CMRI, transthoracic contrast perfusion echocardiography, and dilution techniques. No restrictions were made regarding the study design (randomized, placebo‐controlled/randomized with active comparators/nonrandomized with or without a control group), the cardiac condition studied, or the coronary microvascular function at baseline. An electronic database search yielded 4485 records of which 80 studies met our inclusion criteria. Included studies were sorted according to intervention and study design. Studies were small and heterogeneous in methodology, and only few were placebo‐controlled. Although some treatments looked promising, we found that no specific treatment was sufficiently well documented to be recommended in any patient groups. There is a need for larger well‐designed clinical trials, and we suggest that future studies stratify study populations according to pathogenic mechanisms, thereby investigating whether an individualized treatment approach would be more successful.
PlpA, a PilZ‐like protein, regulates directed motility of the bacterium Myxococcus xanthus
Authors: Pogue Connor B. , Zhou Tianyi , Nan Beiyan 
Published: November 2017

SummaryThe rod‐shaped bacterium Myxococcus xanthus moves on surfaces along its long cell axis and reverses its moving direction regularly. Current models propose that the asymmetric localization of a Ras‐like GTPase, MglA, to leading cell poles determines the moving direction of cells. However, cells are still motile in the mutants where MglA localizes symmetrically, suggesting the existence of additional regulators that control moving direction. In this study, we identified PlpA, a PilZ‐like protein that regulates the direction of motility. PlpA and MglA localize into opposite asymmetric patterns. Deletion of the plpA gene abolishes the asymmetry of MglA localization, increases the frequency of cellular reversals and leads to severe defects in cell motility. By tracking the movements of single motor particles, we demonstrated that PlpA and MglA co‐regulated the direction of gliding motility through direct interactions with the gliding motor. PlpA inhibits the reversal of individual gliding motors while MglA promotes motor reversal. By counteracting MglA near lagging cell poles, PlpA reinforces the polarity axis of MglA and thus stabilizes the direction of motility.