bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Inert and Oxidizing Atmospheres on the Physical and Optical Properties of Luminescent Carbon Dots Prepared through Pyrolysis of a Model Molecule
Authors: Machado Cláudia Emanuele , Tartuci Letícia Gazola , de Fátima Gorgulho Honória , de Oliveira Luiz Fernando Cappa , Bettini Jefferson , Pereira dos Santos Daniela , Ferrari Jefferson Luis , Schiavon Marco Antônio 
Published: February 2016

AbstractThis work used l‐tartaric acid as a model molecule to evaluate how the use of inert and oxidizing atmospheres during pyrolysis affected the physical and optical properties of the resulting carbon dots (CDs). Pyrolysis revealed to be a simple procedure that afforded CDs in a single step, dismissed the addition of organic solvents, and involved only one extraction stage that employed water. By X‐ray diffraction a dependency between the structure of the CDs and the atmosphere (oxidizing or inert) used during the pyrolysis was found. Potentiometric titration demonstrated that the CDs were largely soluble in water; it also aided characterization of the various groups that contained sp3‐hybridized carbon atoms on the surface of the dots. Raman spectroscopy suggested that different amounts of sp2‐ and sp3‐hybridized carbon atoms emerged on the CDs depending on the pyrolysis atmosphere. In conclusion, the pyrolysis atmosphere influenced the physical properties, such as the composition and the final structure.
Fe0 Nanomotors in Ton Quantities (1020 Units) for Environmental Remediation
Authors: Teo Wei Zhe , Zboril Radek , Medrik Ivo , Pumera Martin 
Published: February 2016

AbstractDespite demonstrating potential for environmental remediation and biomedical applications, the practical environmental applications of autonomous self‐propelled micro‐/nanorobots have been limited by the inability to fabricate these devices in large (kilograms/tons) quantities. In view of the demand for large‐scale environmental remediation by micro‐/nanomotors, which are easily synthesized and powered by nontoxic fuel, we have developed bubble‐propelled Fe0 Janus nanomotors by a facile thermally induced solid‐state procedure and investigated their potential as decontamination agents of pollutants. These Fe0 Janus nanomotors, stabilized by an ultrathin iron oxide shell, were fuelled by their decomposition in citric acid, leading to the asymmetric bubble propulsion. The degradation of azo‐dyes was dramatically increased in the presence of moving self‐propelled Fe0 nanomotors, which acted as reducing agents. Such enhanced pollutant decomposition triggered by biocompatible Fe0 (nanoscale zero‐valent iron motors), which can be handled in the air and fabricated in ton quantities for low cost, will revolutionize the way that environmental remediation is carried out.
Pillar[5]arene‐Based Glycoclusters: Synthesis and Multivalent Binding to Pathogenic Bacterial Lectins
Authors: Buffet Kevin , Nierengarten Iwona , Galanos Nicolas , Gillon Emilie , Holler Michel , Imberty Anne , Matthews Susan E. , Vidal Sébastien , Vincent Stéphane P. , Nierengarten Jean‐François 
Published: February 2016

AbstractThe synthesis of pillar[5]arene‐based glycoclusters has been readily achieved by CuAAC conjugations of azido‐ and alkyne‐functionalized precursors. The lectin binding properties of the resulting glycosylated multivalent ligands have been studied by at least two complementary techniques to provide a good understanding. Three lectins were selected from bacterial pathogens based on their potential therapeutic applications as anti‐adhesives, namely LecA and LecB from Pseudomonas aeruginosa and BambL from Burkholderia ambifaria. As a general trend, multivalency improved the binding to lectins and a higher affinity can be obtained by increasing to a certain limit the length of the spacer arm between the carbohydrate subunits and the central macrocyclic core.
A Nitroxide‐Tagged Platinum(II) Complex Enables the Identification of a DNA G‐Quadruplex Binding Mode
Authors: Xu Cui‐Xia , Zhang Xiaojun , Zhou Yi‐Wei , Wang Hanqiang , Cao Qian , Shen Yong , Ji Liang‐Nian , Mao Zong‐Wan , Qin Peter Z. 
Published: February 2016

AbstractWe reported a novel strategy for investigating small molecule binding to G‐quadruplexes (GQs). A newly synthesized dinuclear platinum(II) complex (Pt2L) containing a nitroxide radical was shown to selectively bind a GQ‐forming sequence derived from human telomere (hTel). Using the nitroxide moiety as a spin label, electron paramagnetic resonance (EPR) spectroscopy was carried out to investigate binding between Pt2L and hTel GQ. Measurements indicated that two molecules of Pt2L bind with one molecule of hTel GQ. The inter‐spin distance measured between the two bound Pt2L, together with molecular docking analyses, revealed that Pt2L predominately binds to the neighboring narrow and wide grooves of the G‐tetrads as hTel adopts the antiparallel conformation. The design and synthesis of nitroxide tagged GQ binders, and the use of spin‐labeling/EPR to investigate their interactions with GQs, will aid the development of small molecules for manipulating GQs involved in crucial biological processes.
Structural Re‐engineering of the α‐Helix Mimetic JY‐1‐106 into Small Molecules: Disruption of the Mcl‐1–Bak‐BH3 Protein–Protein Interaction with 2,6‐Di‐Substituted Nicotinates
Authors: Drennen Brandon , Scheenstra Jacob A. , Yap Jeremy L. , Chen Lijia , Lanning Maryanna E. , Roth Braden M. , Wilder Paul T. , Fletcher Steven 
Published: February 2016

AbstractThe disruption of aberrant protein–protein interactions (PPIs) with synthetic agents remains a challenging goal in contemporary medicinal chemistry but some progress has been made. One such dysregulated PPI is that between the anti‐apoptotic Bcl‐2 proteins, including myeloid cell leukemia‐1 (Mcl‐1), and the α‐helical Bcl‐2 homology‐3 (BH3) domains of its pro‐apoptotic counterparts, such as Bak. Herein, we describe the discovery of small‐molecule inhibitors of the Mcl‐1 oncoprotein based on a novel chemotype. Particularly, re‐engineering of our α‐helix mimetic JY‐1‐106 into 2,6‐di‐substituted nicotinates afforded inhibitors of comparable potencies but with significantly decreased molecular weights. The most potent inhibitor 2‐(benzyloxy)‐6‐(4‐chloro‐3,5‐dimethylphenoxy)nicotinic acid (1 r: Ki=2.90 μm) likely binds in the p2 pocket of Mcl‐1 and engages R263 in a salt bridge through its carboxylic acid, as supported by 2D 1H–15N HSQC NMR data. Significantly, inhibitors were easily accessed in just four steps, which will facilitate future optimization efforts.
Deciphering the Conformational Choreography of Zinc Coordination Complexes with Standard and Novel Proton NMR Techniques Combined with DFT Methods
Authors: Pucheta Jose Enrique Herbert , Prim Damien , Gillet Jean Michel , Farjon Jonathan 
Published: February 2016

AbstractThe presence of water has been shown to deeply impact the stability and geometry of Zn complexes in solution. Evidence for tetra‐ and penta‐coordinated species in a pyridylmethylamine–ZnII model complex is presented. Novel 1H NMR tools such as T1‐filtered selective exchange spectroscopy and pure shifted gradient‐encoded selective refocusing as well as classical 2D (1H–1H) exchange spectroscopy, diffusion‐ordered spectroscopy and T1(1H) measurements, in combination with density functional theory methods allow the full conformational dynamics of a pyridylmethylamine–ZnII complex to be revealed. Four conformers and two families of complexes depending on the hydration states are elucidated.
Efficacy of stem cells on the healing of peri‐implant defects: systematic review of preclinical studies
Authors: Misawa Mônica Yuri Orita , Huynh‐Ba Guy , Villar Gustavo Machado , Villar Cristina Cunha 
Published: February 2016

AbstractThis systematic review considers the evidence from animal studies evaluating the effectiveness of mesenchymal stem cells (MSC) in the treatment of intraoral peri‐implant defects. MEDLINE, EMBASE, and LILACS databases were searched for quantitative preclinical controlled animal model studies that evaluated the effect of MSC on bone healing at intraoral peri‐implant bone defects. The primary outcome was the amount of (re‐)osseointegration reported as bone‐to‐implant contact in the defect area. The systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses statement guidelines. Ten studies met the inclusion criteria. Only one study induced peri‐implant inflammation to produce peri‐implant bone defects. In all others, defects were surgically created at implant installation. Differences in defect morphology were identified among the studies. Both xenogenous and autogenous MSC were used to treat peri‐implant defects. These included bone marrow‐derived MSC, periodontal ligament‐derived MSC, umbilical cord MSC, bone marrow‐derived mononuclear cells, and peripheral blood mononuclear cells. Meta‐analysis was not possible because of heterogeneities in study designs. Nonetheless, in most studies, local MSC implantation was not associated with adverse effects and had a positive effect on bone healing around peri‐implant defects. Combination of MSC with membranes and bioactive factors appears to provide improved treatment outcomes. In large animal models, intraoral use of MSC may provide beneficial effects on bone healing within peri‐implant defects. The various degrees of success of MSC in peri‐implant bone healing are likely to be related to the use of cells from various populations, tissues, and donor species. However, human safety and efficacy must be demonstrated before its clinical use can be considered.
Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development
Authors: Sharma Rahul , Ritler Dominic , Meister Peter 
Published: February 2016

SummaryC. elegans has recently emerged as a valuable model to understand the link between nuclear organization and cell fate, by combining microscopy approaches, genome‐wide mapping techniques with advanced genetics. Crucial to these analyses are techniques to determine the genome‐wide interaction pattern of proteins with DNA. Chromatin immunoprecipitation has proven valuable but it requires considerable amounts of starting material. This is sometimes difficult to achieve, in particular for specific genotypes (balanced strains, different sexes, severe phenotypes…). As an alternative to ChIP, DNA adenine methyltransferase identification by sequencing (DamID‐seq) was recently shown to be able to characterize binding sites in single mammalian cells. Additionally, DamID can be achieved for cell‐type specific analysis by expressing Dam fusion proteins under tissue specific promoters in a controlled manner. In this report, we present a user‐friendly pipeline to analyse DamID‐seq data in C. elegans. Based upon this pipeline, we provide a comparative analysis of libraries generated with different starting material and discuss important library features. Moreover, we introduce an adaptation of an imaging based tool to visualize in vivo the cell‐specific tridimensional binding pattern of any protein of interest. genesis 54:151–159, 2016. © 2016 Wiley Periodicals, Inc.
Treatment and survival of patients with insular thyroid carcinoma: 508 cases from the National Cancer Data Base
Authors: Pezzi Todd A. , Sandulache Vlad C. , Pezzi Christopher M. , Turkeltaub Ashley E. , Feng Lei , Cabanillas Maria E. , Williams Michelle D. , Lai Stephen Y. 
Published: February 2016

AbstractBackgroundInsular thyroid carcinoma (ITC) is a rare but aggressive thyroid malignancy.MethodsPatients with ITC (n = 508) reported to the National Cancer Data Base from 1998 to 2012 were evaluated for patient, tumor, and treatment characteristics and outcomes.ResultsCompared to papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC), patients with ITC cancer were older, more often were men, had larger tumors, were more likely to present with distant metastasis, were less likely to have an R0 resection, more likely to receive external beam radiation and chemotherapy, and had significantly worse survival. Multivariate Cox regression identified age >65 years (hazard ratio [HR] = 1.53), presence of at least 1 comorbidity (HR = 1.80), positive lymph nodes (HR = 1.67), the presence of metastasis (HR = 2.73), positive margins (HR = 2.48), and radioactive iodine therapy (HR = 0.63) as significant and independent predictors of survival in ITC.ConclusionTreatment recommendations should incorporate the use of radioactive iodine after complete surgical resection and clearance of involved nodal basins. © 2016 Wiley Periodicals, Inc. Head Neck 38: 906–912, 2016
On the dependence of the saturated hydraulic conductivity upon the effective porosity through a power law model at different scales
Authors: Fallico Carmine , De Bartolo Samuele , Veltri Massimo , Severino Gerardo 
Published: February 2016

AbstractWe study the scale dependence of the saturated hydraulic conductivity Ks through the effective porosity ne by means of a newly developed power‐law model (PLM) which allows to use simultaneously measurements at different scales. The model is expressed as product between a single PLM (capturing the impact of the dominating scale) and a characteristic function κ⋆ accounting for the correction because of the other scale(s). The simple (closed form) expression of the κ⋆‐function enables one to easily identify the scales which are relevant for Ks.The proposed model is then applied to a set of real data taken at the experimental site of Montalto Uffugo (Italy), and we show that in this case two (i.e. laboratory and field) scales appear to be the main ones. The implications toward an important application (solute transport) in Hydrology are finally discussed. Copyright © 2016 John Wiley & Sons, Ltd.
Surface bound VEGF mimicking peptide maintains endothelial cell proliferation in the absence of soluble VEGFin vitro
Authors: Le Saux Guillaume , Plawinski Laurent , Parrot Camila , Nlate Sylvain , Servant Laurent , Teichmann Martin , Buffeteau Thierry , Durrieu Marie‐Christine 
Published: February 2016

AbstractContinuous glucose monitoring is an efficient method for the management of diabetes and in limiting the complications induced by large fluctuations in glucose levels. For this, intravascular systems may assist in producing more reliable and accurate devices. However, neovascularization is a key factor to be addressed in improving their biocompatibility. In this scope, the perennial modification of the surface of an implant with the proangiogenic Vascular Endothelial Growth Factor mimic peptide (SVVYGLR peptide sequence) holds great promise. Herein, we report on the preparation of gold substrates presenting the covalently grafted SVVYGLR peptide sequence and their effect on HUVEC behavior. Effective coupling was demonstrated using XPS and PM‐IRRAS. The produced surfaces were shown to be beneficial for HUVEC adhesion. Importantly, surface bound SVVYGLR is able to maintain HUVEC proliferation even in the absence of soluble VEGF. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1425–1436, 2016.
Recurrent Proximal Femur Fractures in a Teenager With Osteogenesis Imperfecta on Continuous Bisphosphonate Therapy: Are We Overtreating?
Authors: Vasanwala Rashida F , Sanghrajka Anish , Bishop Nicholas J , Högler Wolfgang 
Published: February 2016

ABSTRACT

Long-term bisphosphonate (BP) therapy in adults with osteoporosis is associated with atypical femoral fractures, caused by increased material bone density and prolonged suppression of bone remodeling which may reduce fracture toughness. In children with osteogenesis imperfecta (OI), long-term intravenous BP therapy improves bone structure and mass without further increasing the already hypermineralized bone matrix, and is generally regarded as safe. Here we report a teenage girl with OI type IV, who was started on cyclical intravenous pamidronate therapy at age 6 years because of recurrent fractures. Transiliac bone biopsy revealed classical structural features of OI but unusually low bone resorption surfaces. She made substantial improvements in functional ability, bone mass, and fracture rate. However, after 5 years of pamidronate therapy she started to develop recurrent, bilateral, nontraumatic, and proximal femur fractures, which satisfied the case definition for atypical femur fractures. Some fractures were preceded by periosteal reactions and prodromal pain. Pamidronate was discontinued after 7 years of therapy, following which she sustained two further nontraumatic femur fractures, and continued to show delayed tibial osteotomy healing. Despite rodding surgery, and very much in contrast to her affected, untreated, and normally mobile mother, she remains wheelchair-dependent. The case of this girl raises questions about the long-term safety of BP therapy in some children, in particular about the risk of oversuppressed bone remodeling with the potential for microcrack accumulation, delayed healing, and increased stiffness. The principal concern is whether there is point at which benefit from BP therapy could turn into harm, where fracture risk increases again. This case should stimulate debate whether current adult atypical femoral fracture guidance should apply to children, and whether low-frequency, low-dose cyclical, intermittent, or oral treatment maintenance regimens should be considered on a case-by-case basis. © 2016 American Society for Bone and Mineral Research.
Denitrification coupled with methane oxidation in a membrane bioreactor after methanogenic pre‐treatment of wastewater
Authors: Sánchez Alberto , Rodríguez‐Hernández Leticia , Buntner Dagmara , Esteban‐García Ana L , Tejero Iñaki , Garrido Juan M 
Published: February 2016

AbstractBACKGROUNDThe presence of dissolved methane is an important environmental problem related to greenhouse gas emissions from wastewater treated in methanogenic bioreactors. Methane has a global warming potential of 28. For low‐strength wastewaters, such as sewage, dissolved methane could account for up to 50% of the total methane produced. Dissolved methane is easily desorbed from effluents when released into the environment or post‐treated in aerobic bioreactors. The use of dissolved methane as a carbon source for biological denitrification has been proposed as an alternative for reducing both greenhouse gas emissions and nitrogen concentrations in treated wastewater.RESULTSThe effluent of a methanogenic reactor operating at ambient temperature was post‐treated in a two‐compartment biological membrane bioreactor (MBR). The first compartment consisted of an anoxic denitrification reactor in which dissolved methane was oxidized. The second compartment was an aerobic membrane filtration reactor. Up to 60% nitrogen removal and 95% methane consumption were observed. Batch experiments indicated the presence of microorganisms that were capable of denitrification using dissolved methane.CONCLUSIONSDenitrification using dissolved methane as a carbon source was feasible in an anoxic–aerobic MBR system. Denitrification resulted from a mixture of aerobic and anaerobic methane oxidizing bacteria, anammox and heterotrophic bacteria activity. © 2016 Society of Chemical Industry
Atrial fibrillation associated with acute pancreatitis: a retrospective cohort study in Taiwan
Authors: Lai Shih‐Wei , Lin Cheng‐Li , Liao Kuan‐Fu 
Published: February 2016

AbstractBackgroundThere is little data on the relationship between atrial fibrillation and acute pancreatitis. The aim of this study was to evaluate whether atrial fibrillation is associated with acute pancreatitis.MethodsThis retrospective cohort study analyzed the database of the Taiwan National Health Insurance Program from 2000 to 2010. There were 8,981 subjects aged 20–84 with newly diagnosed atrial fibrillation as the atrial fibrillation group and 26,643 sex‐ and age‐matched, randomly selected subjects without atrial fibrillation as the non‐atrial fibrillation group. We used the multivariable Cox proportional hazards regression model to measure the hazard ratio (HR) and 95% confidence interval (CI) for acute pancreatitis risk associated with atrial fibrillation and other comorbidities.ResultsThe crude incidence rates of acute pancreatitis were 3.07 per 1,000 person‐years in the atrial fibrillation group and 2.03 per 1,000 person‐years in the non‐atrial fibrillation group (incidence rate ratio 1.51, 95% CI 1.40, 1.63). After controlling for potential confounding factors, the adjusted HR of acute pancreatitis was 1.46 for the atrial fibrillation group, as compared with the non‐atrial fibrillation group (95% CI 1.18, 1.80). In further analysis, even in the absence of alcohol‐related disease and biliary stone, the adjusted HR of acute pancreatitis was 1.62 for the atrial fibrillation group, as compared with the non‐atrial fibrillation group (95% CI 1.24, 2.12).ConclusionsAtrial fibrillation is associated with acute pancreatitis in the Taiwanese population, even in the absence of alcohol‐related disease and biliary stone. Further research is necessary to explore the pathogenesis underlying the relationship between atrial fibrillation and acute pancreatitis.
Multifaceted intervention including education, rounding checklist implementation, cost feedback, and financial incentives reduces inpatient laboratory costs
Authors: Yarbrough Peter M. , Kukhareva Polina V. , Horton Devin , Edholm Karli , Kawamoto Kensaku 
Published: February 2016

BACKGROUNDInappropriate laboratory testing is a contributor to waste in healthcare.OBJECTIVETo evaluate the impact of a multifaceted laboratory reduction intervention on laboratory costs.DESIGNA retrospective, controlled, interrupted time series (ITS) study.SETTINGUniversity of Utah Health Care, a 500‐bed academic medical center in Salt Lake City, Utah.POPULATIONAll patients 18 years or older admitted to the hospital to a service other than obstetrics, rehabilitation, or psychiatry.INTERVENTIONMultifaceted quality‐improvement initiative in a hospitalist service including education, process change, cost feedback, and financial incentive.MEASUREMENTSPrimary outcomes of lab cost per day and per visit. Secondary outcomes of number of basic metabolic panel (BMP), comprehensive metabolic panel (CMP), complete blood count (CBC), and prothrombin time/international normalized ratio tests per day; length of stay (LOS); and 30‐day readmissions.RESULTSA total of 6310 hospitalist patient visits (intervention group) were compared to 25,586 nonhospitalist visits (control group). Among the intervention group, the unadjusted mean cost per day was reduced from $138 before the intervention to $123 after the intervention (P < 0.001), and the unadjusted mean cost per visit decreased from $618 to $558 (P = 0.005). The ITS analysis showed significant reductions in cost per day, cost per visit, and the number of BMP, CMP, and CBC tests per day (P = 0.034, 0.02, <0.001, 0.004, and <0.001). LOS was unchanged and 30‐day readmissions decreased in the intervention group.CONCLUSIONA multifaceted approach to laboratory reduction demonstrated a significant reduction in laboratory cost per day and per visit, as well as common tests per day at a major academic medical center. Journal of Hospital Medicine 2016;11:348–354. © 2016 Society of Hospital Medicine