bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distributed device association for multiservice heterogeneous cellular networks with QoS provisioning
Authors: Elhattab M. K. , Elmesalawy Mahmoud M. , Ibrahim I. I. 
Published: June 2017

AbstractHeterogeneous cellular network offers a promising architecture for supporting multiservice traffic generated by Internet of thing (IoT) paradigm and human‐to‐human (H2H) communications. In this paper, a distributed device association algorithm is proposed to consider the diverse quality of service requirements for H2H devices and IoT devices coexisted in heterogeneous cellular network. In this context, the traffic generated by H2H devices and IoT devices is categorized into 2 main classes, ie, primary service class (PSC) requiring low outage probability and secondary service class (SSC) with a minimum long‐term rate requirement. Primary service class is principally used to model H2H traffic, while SSC is mainly used to model IoT traffic. The association problem is formulated to maximize the system revenue in terms of the number of devices belonging to the SSC while minimizing the global outage probability for devices modeled to PSC under quality of service guarantee. Since the proposed optimization problem is an NP combinatorial problem, an efficient solution is derived by relaxing the association constraints and adopting the Lagrange dual decomposition method. Simulation results show that our proposed algorithm outperforms the comparable schemes especially on outage performance for PSC‐modeled devices and number of accommodated SSC‐modeled devices.
Interferon‐β regulates the production of IL‐10 by toll‐like receptor‐activated microglia
Authors: Lobo‐Silva Diogo , Carriche Guilhermina M. , Castro A. Gil , Roque Susana , Saraiva Margarida 
Published: June 2017

AbstractPattern recognition receptors, such as toll‐like receptors (TLRs), perceive tissue alterations and initiate local innate immune responses. Microglia, the resident macrophages of the brain, encode TLRs which primary role is to protect the tissue integrity. However, deregulated activation of TLRs in microglia may lead to chronic neurodegeneration. This double role of microglial responses is often reported in immune‐driven neurologic diseases, as in multiple sclerosis (MS). Consequently, strategies to manipulate microglia inflammatory responses may help to ameliorate disease progression. In this context, the anti‐inflammatory cytokine interleukin (IL)‐10 appears as an attractive target. In this study, we investigated how activation of microglia by TLRs with distinct roles in MS impacts on IL‐10 production. We found that activation of TLR2, TLR4, and TLR9 induced the production of IL‐10 to a greater extent than activation of TLR3. This was surprising as both TLR3 and IL‐10 play protective roles in animal models of MS. Interestingly, combination of TLR3 triggering with the other TLRs, enhanced IL‐10 through the modulation of its transcription, via interferon (IFN)‐β, but independently of IL‐27. Thus, in addition to the modulation of inflammatory responses of the periphery described for the axis TLR3/IFN‐β, we now report a direct modulation of microglial responses. We further show that the presence of IFN‐γ in the microenvironment abrogated the modulation of IL‐10 by TLR3, whereas that of IL‐17 had no effect. Considering the therapeutic application of IFN‐β in MS, our study bears important implications for the understanding of the cytokine network regulating microglia responses in this setting.
Schwann cell‐specific deletion of the endosomal PI 3‐kinase Vps34 leads to delayed radial sorting of axons, arrested myelination, and abnormal ErbB2‐ErbB3 tyrosine kinase signaling
Authors: Logan Anne M. , Mammel Anna E. , Robinson Danielle C. , Chin Andrea L. , Condon Alec F. , Robinson Fred L. 
Published: June 2017

AbstractThe PI 3‐kinase Vps34 (Pik3c3) synthesizes phosphatidylinositol 3‐phosphate (PI3P), a lipid critical for both endosomal membrane traffic and macroautophagy. Human genetics have implicated PI3P dysregulation, and endosomal trafficking in general, as a recurring cause of demyelinating Charcot‐Marie‐Tooth (CMT) peripheral neuropathy. Here, we investigated the role of Vps34, and PI3P, in mouse Schwann cells by selectively deleting Vps34 in this cell type. Vps34‐Schwann cell knockout (Vps34SCKO) mice show severe hypomyelination in peripheral nerves. Vps34–/– Schwann cells interact abnormally with axons, and there is a delay in radial sorting, a process by which large axons are selected for myelination. Upon reaching the promyelinating stage, Vps34–/– Schwann cells are significantly impaired in the elaboration of myelin. Nerves from Vps34SCKO mice contain elevated levels of the LC3 and p62 proteins, indicating impaired autophagy. However, in the light of recent demonstrations that autophagy is dispensable for myelination, it is unlikely that hypomyelination in Vps34SCKO mice is caused by impaired autophagy. Endosomal trafficking is also disturbed in Vps34–/– Schwann cells. We investigated the activation of the ErbB2/3 receptor tyrosine kinases in Vps34SCKO nerves, as these proteins, which play essential roles in Schwann cell myelination, are known to traffic through endosomes. In Vps34SCKO nerves, ErbB3 was hyperphosphorylated on a tyrosine known to be phosphorylated in response to neuregulin 1 exposure. ErbB2 protein levels were also decreased during myelination. Our findings suggest that the loss of Vps34 alters the trafficking of ErbB2/3 through endosomes. Abnormal ErbB2/3 signaling to downstream targets may contribute to the hypomyelination observed in Vps34SCKO mice.
Novel psychoactive substance use among treatment‐seeking opiate users: The role of life events and psychiatric symptoms
Authors: Kapitány‐Fövény Máté , Farkas Judit , Pataki Patrícia Andrea , Kiss Anna , Horváth Janka , Urbán Róbert , Demetrovics Zsolt 
Published: June 2017

AbstractObjectiveOver the past 5 years, a shift to the use of novel psychoactive substances (NPS) has been observed among opioid users. The aim of this study was to assess the potential reasons for NPS use among treatment‐seeking patients receiving opiate substitution therapy.MethodA structured questionnaire was filled out by a sample of 198 opiate dependent patients of Hungary's National Institute of Psychiatry and Addiction. This questionnaire assessed demographics, treatment characteristics, lifetime substance use, potential reasons for NPS use, negative life events (Life Events Scale), and psychiatric symptoms (Brief Symptom Inventory).ResultsThe most frequent reasons for NPS use were curiosity, replacing other drugs, and easy availability. The majority of the respondents used synthetic cathinones and chose practical reasons, not psychopharmacological preferences. A series of binary logistic regressions indicated that lifetime amphetamine use (OR = 4.64, 95% CI [2.16, 9.96]) and more severe psychiatric symptoms (OR = 1.89, 95% CI [1.18, 3.04]) may predict NPS use. Time spent in treatment was a minor protective factor (OR = 0.92, 95% CI [0.86, 0.99]).ConclusionSynthetic cathinones might still substitute amphetamine‐derivatives, although these NPS are no longer legal. There is a need for the regular screening of psychiatric symptoms and the use of family therapy among participants on opioid substitution programs.
THE TRANSITION FROM MOTHER‐OF‐ONE TO MOTHER‐OF‐TWO: MOTHERS’ PERCEPTIONS OF THEMSELVES AND THEIR RELATIONSHIPS WITH THEIR FIRSTBORN CHILDREN
Authors: Chapman Jennifer K. , Hart Sybil L. 
Published: June 2017

ABSTRACTThis qualitative study aimed to explore mothers’ perceptions of the transition to second‐time motherhood, and advance methodology for supporting mothers during this transition. Fifty‐seven pregnant mothers and their firstborn children (M age = 30.3 months) participated in a laboratory procedure in which the mother exposed her child to a brief episode of differential treatment. The episode was designed to simulate an expected experience of caregiving that would occur after the birth of a second child. During a postnatal visit (M=5.72 weeks following childbirth), mothers provided written feedback on their perceptions of the transition and the contribution of the laboratory experience to that transition. Qualitative content analysis revealed that mothers approached the birth of a second child with apprehension. Intrapersonal themes revealed that participation in the procedure shaped mothers’ understanding of their expanded maternal roles by enhancing their sense of preparedness and confidence in their abilities to support their children. Interpersonal themes revealed that participation helped mothers appreciate the magnitude of the challenge that their children would face and gave mothers reason to believe that their children would adjust successfully. Findings are discussed in terms of their potential contribution toward developing methodology for helping mothers prepare for the addition of a second child.
Transcriptomic Profiling in Human Decidua of Severe Preeclampsia Detected by RNA Sequencing
Authors: Tong Jing , Zhao Weixiu , Lv Hong , Li Wei‐Ping , Chen Zi‐Jiang , Zhang Cong 
Published: June 2017

ABSTRACTMaternal decidua plays a critical role in implantation and placentation. Impaired decidualization causes failed intravascular trophoblast invasion and inadequate placentation and then increases the risk of preeclampsia (PE). RNA sequencing (RNA‐Seq) has achieved great advances in the characterization and quantification of transcriptomes; is a powerful tool for new transcript discovery, genome annotation, and expression profiling. In the present study, we conducted a RNA‐Seq analysis to compare gene expression between decidua of PE (n = 3, early‐onset severe PE, EOSPE; n = 3, late‐onset severe PE, LOSPE) and normal pregnancies (n = 3). We revealed that decidual gene transcription profile was altered in severe PE and identified 293 key PE‐related genes involved in 19 differentially regulated pathways relevant for the pathogenesis of PE, among which ENO2, PGK1, and HK2 involved in glycolysis/gluconeogenesis and HIF‐1 signaling pathway which are all highly related with tumorigenesis and are significantly upregulated in cancer cells were severely inhibited in the decidua of PE. Moreover, we identified 22 core regulatory genes, including the newly identified pseudogenes BNIP3P1, HK2P1, and PGK1P1 that encode long non‐coding RNA (lncRNA); interestingly, BNIP3/BNIP3P1, HK2/HK2P1, and PGK1/PGK1P1 appear in pairs in core genes. Subsequent analyses using quantitative PCR validated a portion of these results. This study may provide further insight into the mechanisms of PE and function as preventive, predictive, and therapeutic measures. Future functional studies are needed in order to accomplish a greater understanding of the mechanisms involved. J. Cell. Biochem. 119: 607–615, 2018. © 2017 Wiley Periodicals, Inc.
Reduced expression of circRNA hsa_circ_0003159 in gastric cancer and its clinical significance
Authors: Tian Mengqian , Chen Ruoyu , Li Tianwen , Xiao Bingxiu 
Published: June 2017

BackgroundCircular RNAs (circRNAs) play a crucial role in the occurrence of several diseases including cancers. However, little is known about circRNAs’ diagnostic values for gastric cancer, one of the worldwide most common diseases of mortality.MethodsThe hsa_circ_0003159 levels in 108 paired gastric cancer tissues and adjacent non‐tumorous tissues from surgical patients with gastric cancer were first detected by real‐time quantitative reverse transcription‐polymerase chain reaction. Then, the relationships between hsa_circ_0003159 expression levels in gastric cancer tissues and the clinicopathological factors of patients with gastric cancer were analyzed. Finally, its diagnostic value was evaluated through the receiver operating characteristic curve.ResultsCompared with paired adjacent non‐tumorous tissues, hsa_circ_0003159 expression was significantly down‐regulated in gastric cancer tissues. What is more, we found that hsa_circ_0003159 expression levels were significantly negatively associated with gender, distal metastasis, and tumor‐node‐metastasis stage.ConclusionsAll of the results suggest that hsa_circ_0003159 may be a potential cancer marker of patients with gastric cancer.
BACE1 Inhibitor Lanabecestat (AZD3293) in a Phase 1 Study of Healthy Japanese Subjects: Pharmacokinetics and Effects on Plasma and Cerebrospinal Fluid Aβ Peptides
Authors: Sakamoto Kei , Matsuki Shunji , Matsuguma Kyoko , Yoshihara Tatsuya , Uchida Naoki , Azuma Fumihiko , Russell Muir , Hughes Glen , Haeberlein Samantha Budd , Alexander Robert C. , Eketjäll Susanna , Kugler Alan R. 
Published: June 2017

AbstractLanabecestat (AZD3293; LY3314814) is an orally active potent inhibitor of human β‐secretase 1 in clinical development for the treatment of Alzheimer disease. In this first Japanese clinical study for an Alzheimer disease intervention to include cerebrospinal fluid (CSF) sampling in Japanese elderly healthy subjects, we report the pharmacokinetics and effects on plasma and CSF amyloid‐β (Aβ) peptides of lanabecestat in a phase 1 study involving 40 healthy Japanese subjects (NCT02005211). No safety and tolerability concerns were identified in healthy Japanese subjects exposed to lanabecestat up to the highest doses given, which is consistent with observations in a US phase 1 study of lanabecestat. Exposure to lanabecestat was similar for young and elderly subjects and increased in a dose‐dependent manner. For elderly subjects, plasma lanabecestat half‐life after multiple dosing was 12 to 17 hours (on days 10 and 14). Robust plasma and CSF Aβ peptide reductions were also seen at all doses, with CSF Aβ42 concentrations reduced by 63% and 79% in the 15‐ and 50‐mg lanabecestat groups, respectively. CSF soluble amyloid‐β precursor protein β also decreased following lanabecestat treatment. Suppression of CSF Aβ peptides was similar in elderly healthy Japanese subjects and US patients with mild to moderate Alzheimer disease. Lanabecestat is a promising potentially disease‐modifying treatment in phase 3 development for patients with early Alzheimer disease.
Algal biomass as fuel for stacked‐MFCs for profitable, sustainable and carbon neutral bioenergy generation
Authors: Asensio Yeray , Fernandez‐Marchante Carmen , Villaseñor Jose , Lobato Justo , Cañizares Pablo , Rodrigo Manuel A 
Published: June 2017

AbstractBACKGROUNDThis work compares the performance of three stacked microbial fuel cells constructed with different number of single‐MFC (MFC1 with two stacked‐MFCs, MFC2 with ten stacked‐MFCs and MFC3 with twenty stacked‐MFCs), and operated under the same conditions for one month.RESULTSAccording to results, algae suspensions can be used as fuel for MFC‐stacks, although current efficiencies obtained are low. In comparing the effect of number of cells stacked on the performance of the stacks, it was found that the higher the number of cells stacked, the higher the energy harvested from algae. However, because of the very efficient consumption of COD in the first MFC of the stacks (not only by electrogenic but also by non‐electrogenic microorganisms) and the sequential circulation of the fuel through the different cells of the stack, in all cases the systems ran out of fuel and this was reflected in lower production of electricity, compared with that expected taking into account the number of cells stacked. Results obtained from the polarization curves and the cathodic oxygen consumption also support this explanation.CONCLUSIONSResults demonstrate that algal biomass is a suitable fuel for energy generation using MFC technology and provides microorganisms not only of a carbon source but also with the required nutrients. However, the low coulombic efficiencies obtained in the three stacks indicate that feeding algae to MFC also promotes the formation of an important amount of non‐electrogenic microorganisms that compete successfully with bioelectrogenic microorganisms for the substrate provided. © 2017 Society of Chemical Industry
Evaluating effectiveness of infection control efforts in hospitals using information in microbiological laboratory databases
Authors: Saito Norihiro , Itoga Masamichi , Kimura Masahiko , Inoue Fumio , Minakawa Satoko , Kimura Toshiyuki , Ozaki Hiromi , Saito Yumiko , Takahashi Mikiko , Fujishima Tetsuhiro , Mizuno Sumie , Ogawa Shin , Kitayama Yuko , Kudo Kazumi , Minami Kazushi , Abo Fumiko , Takano Yasuyuki , Ohdaira Naotake , Hamada Satoshi , Ueki Shigeharu , Hirokawa Makoto , Kayaba Hiroyuki 
Published: June 2017

AbstractPurposeTo analyze the quality of infection control activities, bacteriological data relevant to infection control was evaluated through the microbiological data warehouse networking hospitals in two medical regions.MethodsData regarding bacterial test results of 19 hospitals were extracted from two microbiological laboratory information data bases. The rate of MRSA among total S. aureus was used as a general indicator of infection control activities. The occupancy rate of nasal or pharyngeal swabs among MRSA‐positive bacteriological samples was used as an indicator of attention paid for infection control in intensive care wards. The number of blood culture sets per examined patient was utilized as an indicator for life‐long vocational education on updated medical practice relevant to infectious diseases.ResultsThe rate of MRSA was significantly higher in secondary private hospitals. The occupancy rate of nasal or pharyngeal swabs was significantly higher in tertiary hospitals. The average number of blood culture set per examined patient were 1.55, 1.54 and 1.39 in tertiary, secondary public and secondary private hospitals, respectively; however, there were no statistical differences between groups.ConclusionsData bases of microbiological test results shared by hospital laboratories are useful for evaluating regional infection control activities.
Solid‐phase extraction assisted dispersive liquid–liquid microextraction based on solidification of floating organic droplet to determine sildenafil and its analogues in dietary supplements
Authors: Li Jing , Roh Si Hun , Shaodong Jia , Hong Ji Yeon , Lee Dong‐Kyu , Shin Byong‐kyu , Park Jeong Hill , Lee Jeongmi , Kwon Sung Won 
Published: June 2017

A novel analytical method for the simultaneous determination of the concentration of sildenafil and its five analogues in dietary supplements using solid‐phase extraction assisted reversed‐phase dispersive liquid–liquid microextraction based on solidification of floating organic droplet combined with ion‐pairing liquid chromatography with an ultraviolet detector was developed. Parameters that affect extraction efficiency were systematically investigated, including the type of solid‐phase extraction cartridge, pH of the extraction environment, and the type and volume of extraction and dispersive solvent. The method linearity was in the range of 5.0–100 ng/mL for sildenafil, homosildenafil, udenafil, benzylsildenafil, and thiosildenafil and 10–100 ng/mL for acetildenafil. The coefficients of determination were ≥0.996 for all regression curves. The sensitivity values expressed as limit of detection were between 2.5 and 7.5 ng/mL. Furthermore, intraday and interday precisions expressed as relative standard deviations were less than 5.7 and 9.9%, respectively. The proposed method was successfully applied to the analysis of sildenafil and its five analogues in complex dietary supplements.
Bis(trifluoromethylsulfonyl)imide‐based frozen ionic liquid for the hollow‐fiber solid‐phase microextraction of dichlorodiphenyltrichloroethane and its main metabolites
Authors: Pang Long , Yang Peijie , Pang Rong , Li Shunyi 
Published: June 2017

1‐Hexadecyl‐3‐methylimidazolium bis(trifluoromethylsulfonyl)imide is a solid‐phase ionic organic material under ambient temperature and is considered as a kind of “frozen” ionic liquid. Because of their solid‐state and ultra‐hydrophobicity, “frozen” ionic liquids are able to be confined in the pores of hollow fiber, based on which a simple method was developed for the hollow‐fiber solid‐phase microextraction of dichlorodiphenyltrichloroethane and its main metabolites. Under optimized conditions, the proposed method results in good linearity (R2 > 0.9965) over the range of 0.5−50 μg/L, with low limits of detection and quantification in the range of 0.33−0.38 and 1.00−1.25 μg/L, respectively. Intra‐ and interday precisions evaluated by relative standard deviation were 3−6 and 1−6%, respectively. The spiked recoveries of dichlorodiphenyltrichloroethane and its main metabolites from real water samples were in the range of 64−113 and 79−112%, respectively, at two different concentration levels. The results suggest that “frozen” ionic liquids are promising for use as a class of novel sorbents.
Identification of an Alu element‐mediated deletion in the promoter region of GNE in siblings with GNE myopathy
Authors: Garland Jennifer , Stephen Joshi , Class Bradley , Gruber Angela , Ciccone Carla , Poliak Aaron , Hayes Christina P. , Singhal Vandana , Slota Christina , Perreault John , Gavrilova Ralitza , Shrader Joseph A. , Chittiboina Prashant , Joe Galen , Heiss John , Gahl William A. , Huizing Marjan , Carrillo Nuria , Malicdan May Christine V. 
Published: June 2017

AbstractBackgroundGNE myopathy is a rare genetic disease characterized by progressive muscle atrophy and weakness. It is caused by biallelic mutations in the GNE gene that encodes for the bifunctional enzyme, uridine diphosphate (UDP)‐N‐acetylglucosamine (GlcNAc) 2‐epimerase/N‐acetylmannosamine (ManNAc) kinase. Typical characteristics of GNE myopathy include progressive myopathy, first involving anterior tibialis muscle and sparing the quadriceps, and rimmed vacuoles on muscle biopsy. Identifying biallelic mutations by sequencing of the GNE gene confirms the diagnosis of GNE myopathy. In a subset of patients, diagnostic confirmation is challenged by the identification of mutations in only one allele, suggesting mutations in deep intronic regions or regulatory regions.MethodsWe performed targeted sequencing and copy number variant (CNV) analysis of GNE in two siblings who clinically presented with GNE myopathy. Further molecular and biochemical studies were done to characterize the effect of a previously uncharacterized GNE mutation.ResultsWe report two siblings of Indian descent with characteristic features of GNE myopathy, including progressive skeletal muscle weakness initially involving the anterior tibialis, and rimmed vacuoles on muscle biopsy, in which a heterozygous mutation, p.Val727Met, was identified in both affected siblings, but no other deleterious variants in either coding region or exon–intron boundaries of the gene. Subsequent insertion/deletion analysis identified a novel 11.3‐kb deletion (Chr9 [GRCh37]: g.36257583_36268910del) encompassing the GNE promoter region, with breakpoints residing in Alu repeats. Gene expression analysis revealed reduced GNE mRNA and protein levels, confirming decreased expression of the deleted allele harboring the deletion.ConclusionsWe have identified GNE as one of the genes susceptible to Alu‐mediated recombination. Our findings suggest that the deletion may encompass the promoter or another region necessary for GNE expression. In patients with typical manifestations of GNE myopathy and a single GNE variant identified, copy number variant (CNV) analysis may be useful in arriving at the diagnosis.