bluuebunny/crossref_metadata_2025_split huggingface dataset:
A genome-wide One Health study ofKlebsiella pneumoniaein Norway reveals overlapping populations but few recent transmission events across reservoirs
Authors: Hetland Marit A K , Winkler Mia A , Kaspersen Hako , Håkonsholm Fredrik , Bakksjø Ragna-Johanne , Bernhoff Eva , Delgado-Blas Jose F. , Brisse Sylvain , Correia Annapaula , Fostervold Aasmund , Lam Margaret M C , Lunestad Bjørn-Tore , Marathe Nachiket P , Raffelsberger Niclas , Samuelsen Ørjan , Sunde Marianne , Sundsfjord Arnfinn , Urdahl Anne Margrete , Wick Ryan R , Löhr Iren H , Holt Kathryn E 
Published: September 2024

AbstractMembers of theKlebsiella pneumoniaespecies complex (KpSC) are opportunistic pathogens that cause severe and difficult-to-treat infections. KpSC are common in non-human niches, but the clinical relevance of these populations is disputed. Utilising 3,255 whole-genome sequenced isolates from human, animal and marine sources collected during 2001-2020 in Norway, we showed the KpSC populations in different niches were distinct but overlapping. Notably, human infection isolates showed greatest connectivity with each other, followed by isolates from human carriage, pigs, and bivalves. Nearly 5% of human infection isolates had close relatives (≤22 substitutions) amongst animal and marine isolates, despite temporally and geographically distant sampling of these sources. Infection prevention measures are essential to limit transmission within human clinical settings and reduce disease burden. However, as colonisation often precedes infection, preventing transmission that leads to colonisation, e.g. transmission between animals and humans in the community, and in the food chain, could also be beneficial.
The oocyte zinc transporterSlc39a10/Zip10is a regulator of zinc sparks during fertilization in mice
Authors: Kageyama Atsuko , Ogonuki Narumi , Wakai Takuya , Namiki Takafumi , Kawata Yui , Ozawa Manabu , Yamada Yasuhiro , Fukada Toshiyuki , Ogura Atsuo , Fissore Rafael A. , Kashiwazaki Naomi , Ito Junya 
Published: September 2024

AbstractIn all vertebrates studied to date, a rise(s) in intracellular calcium is indispensable for successful fertilization and further embryonic development. Recent studies demonstrated that zinc is ejected to the extracellular milieu, the ‘zinc spark’, and follows the first few calcium rises of fertilization. However, the role of the zinc sparks in fertilization and development, and the supporting influx mechanism(s) are unknown. In this study in mouse oocytes, we investigated the role of zinc transportersZip6/Slc39a6andZip10/Slc39a10. Zip10 mRNA or ZIP10 was expressed throughout folliculogenesis in oocyte or the plasma membrane, respectively. ZIP6 was also expressed in nuclear localization in oocytes and granulosa cells throughout folliculogenesis. The number of ovulated oocytes was examined in oocyte-specificZip6(Zip6d/d:Zip6flox/floxGdf9Cre/+) andZip10(Zip10d/d:Zip10flox/floxGdf9Cre/+) knockout mice, and no change was observed for either strain.Zip10d/doocytes matured and formed normal metaphase II spindles, but had lower labile zinc levels as suggested by the zinc indicator, Fluozin-3 intensity. The levels of zinc fluorescence intensity in theZip6d/dgroup were not different from theZip6f/f. Fertilization-induced calcium oscillations were present in bothZip6d/dandZip10d/doocytes, but zinc sparks were observed inZip6d/dbut not inZip10d/doocytes. Despite other events of egg activation proceeding normally inZip10d/doocytes, embryo development into blastocysts was compromised. We show here for the first time that the zinc transporter ZIP10 contributes to zinc homeostasis in oocytes and embryos, highlighting the pivotal role of this divalent cation in early development.ClassificationMajor Classification: Biological SciencesMinor Classification: Developmental BiologySignificance statementRecent studies reported that the ejection of zinc into the extracellular milieu also called the ‘zinc spark’, occurs during mammalian fertilization including humans and all vertebrates. However, the role and molecules involved in producing these sparks remain unclear. We demonstrated for the first time that a zinc transporter,Slc39a10/Zip10,is required for the zinc sparks of fertilization in mice. In addition, ZIP10-dependent zinc signaling is not involved in the zona reaction or the block of polyspermy but it is in embryonic development. Taken together, we show here a zinc transporter-dependent signaling event has pivotal roles in fertilization and embryonic development in mice. Our findings will contribute to improving embryonic development in all mammals.
Large-scale mouse mutagenesis identifies novel genes affecting vertebral development
Authors: Ibarra-Soria Ximena , Webb Elizabeth , Mulley John F. 
Published: September 2024

AbstractWe analyzed the International Mouse Phenotyping Consortium (IMPC) release 19 set of 8,539 phenotyped whole-gene knockouts to identify 204 genes that alter vertebral development. These genes are broadly grouped into six categories based on their phenotype: “vertebral number” (22 genes); “vertebral processes” (35 genes); “spine shape” (16 genes); “tail morphology” (73 genes); “vertebral form” (62 genes); and “somitogenesis” (24 genes), with minimal overlap between groups. Gene expression analysis of somite trios across six developmental stages show that 182 of these genes are expressed in somites, and 60% of them show variable expression during somite maturation. A further 54% show expression changes between developmental stages. Fourteen of the 204 genes affecting vertebral development have a vertebral phenotype as their only phenotype, and for 34 genes vertebral phenotypes represent ≥50% of their total phenotypes. We find no evidence for a previous association of the majority of these genes with vertebral defects, and have therefore identified an extensive set of novel candidate genes for association with vertebral malformations in humans, including vertebral fusions, numerical variation, and scoliosis.
Ternary structure of Plasmodium vivax
N-myristoyltransferase with myristoyl-CoA and inhibitor IMP-0001173
Authors: Bolling Cydni , Mendez Alex , Taylor Shane , Makumire Stanley , Reers Alexandra , Zigweid Rachael , Subramanian Sandhya , Dranow David M. , Staker Bart , Edwards Thomas E. , Tate Edward W. , Bell Andrew S. , Myler Peter J. , Asojo Oluwatoyin A. , Chakafana Graham 
Published: September 2024

Plasmodium vivax is a major cause of malaria, which poses an increased health burden on approximately one third of the world's population due to climate change. Primaquine, the preferred treatment for P. vivax malaria, is contraindicated in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency, a common genetic cause of hemolytic anemia, that affects ∼2.5% of the world's population and ∼8% of the population in areas of the world where P. vivax malaria is endemic. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) conducted a structure–function analysis of P. vivax
N-myristoyltransferase (PvNMT) as part of efforts to develop alternative malaria drugs. PvNMT catalyzes the attachment of myristate to the N-terminal glycine of many proteins, and this critical post-translational modification is required for the survival of P. vivax. The first step is the formation of a PvNMT–myristoyl–CoA binary complex that can bind to peptides. Understanding how inhibitors prevent protein binding will facilitate the development of PvNMT as a viable drug target. NMTs are secreted in all life stages of malarial parasites, making them attractive targets, unlike current antimalarials that are only effective during the plasmodial erythrocytic stages. The 2.3 Å resolution crystal structure of the ternary complex of PvNMT with myristoyl-CoA and a novel inhibitor is reported. One asymmetric unit contains two monomers. The structure reveals notable differences between the PvNMT and human enzymes and similarities to other plasmodial NMTs that can be exploited to develop new antimalarials.
A trusted B2B relationship quality in the Asian market: effects of salespeople's attributes
Authors: Lii Yuan-Shuh , Ding May-Ching , Lee Shanchih 
Published: September 2024

PurposeThe research applied service-dominant logic of marketing theory, a customer-centered and relational that principally grounded in service relationships and actor networks. In actor networks, salespeople provide their skills and knowledge, such as expertise, service quality, ethics and shared value to cocreate value for buyers. Therefore, this study explores the attributes of salespeople that influence the quality of the relationship (trust and satisfaction) and, as a result, loyalty in the context of the business-to-business (B2B) relationship in the Taiwan market.Design/methodology/approachA causal relationship and survey research design are applied. The study collected 266 valid responses from B2B account managers representing various companies and industries. Structural equation modeling (SEM) was applied to test the hypotheses.FindingsThe results showed that salespeople’s expertise demonstrated the most significant influence on both trust and satisfaction, followed by ethics, service quality and share value, in a descending order of impact. Consequently, trust and satisfaction had a significant impact on customer loyalty.Practical implicationsThe four attributes of salespeople play a pivotal role in establishing lasting relationships and maximizing the customer lifetime value. To achieve long-term success in customer interactions and relationships, a well-rounded salesperson should diligently strive to excel in all these attributes.Originality/valueThe novelty and contribution of this study are twofold. First, investigating the quality of the relationship in the context of Taiwanese manufacturers in a B2B setting is still rare, and this is the study first to explore the Taiwanese B2B relationship with its global customers. As Taiwanese manufacturers play a pivotal role in the global supply chain, the research findings have symbolic meaning and practical implications for global business partners. Second, drawing from service-dominant logic theory, this research takes an integrative view by examining the attributes (expertise, service quality, shared value and ethics) that influence and establish a quality trusting relationship and consumer loyalty in the B2B context.
Understanding the political connections of Portuguese companies through their board members
Authors: Ferreira Paulo , Oliveira Jonas , Azevedo Graça 
Published: September 2024

PurposeThis study aims to analyse the political connections of Portuguese companies through the members of the board of directors, exploring how these connections influence, in particular, the composition and characteristics of the boards.Design/methodology/approachThe research used a strategy based on analysing the financial statements and curriculum vitae of the directors of Portuguese companies listed on Euronext Lisbon from 2014 to 2019. The political connections of board members were examined, considering the variables identified in the existing literature.FindingsThe results indicate that companies with political connections maintain these relationships for long periods and have a greater number of members on the board of directors compared to companies without such connections. Directors with political experience tend to occupy non-executive positions, suggesting that companies may value political contacts more than the management skills of these directors. It was also found that there are politically connected directors who belong to multiple boards and that women appointed to the board are less likely to have a political background, reflecting male dominance in Portuguese politics.Research limitations/implicationsThe main limitations of this study include the small number of listed companies in the sample, which may affect the statistical robustness of the results, as well as the use of secondary sources, which may not capture all relevant policy linkages. In addition, the results are specific to the Portuguese context and may not be generalisable to other countries or other regions of the world.Originality/valueThis study contributes to the understanding of political connections in Portuguese companies, offering valuable insights into how these connections influence board composition and can impact corporate strategy and governance. The findings of this study can be especially useful for business leaders looking to optimise the formation of their boards of directors.
Size matters: the influence of supplier size on buyer's usage of mediated power in positive and negative supplier-induced disruptions
Authors: Chu Yu (Jade) , Duan Yanji 
Published: September 2024

PurposeWhile size asymmetry in buyer–supplier relationships has been studied in non-disruption contexts, this research explores how supplier size influences positive and negative supply chain disruptions. Anchoring on the commitment-trust theory (CTT), we explore buyer commitment as a mediating variable and examine how buying firms' mediated power usage depends on different supplier sizes and types of supplier-induced disruptions.Design/methodology/approachThrough two scenario-based behavioral experiments, we discover different patterns in buyers' use of mediated power, contingent on the types of supplier-induced disruptions.FindingsIn negative disruptions, buyers prefer more mediated power with large suppliers to control uncertainties, using reward or coercive power strategies. In positive disruptions, we find opposite results, indicating different buyers' perceptions and actions are contingent on both the supplier size and the types of disruptions. These findings underscore the complex interplay between supplier size, buyer commitment and mediated power strategies, revealing that disruption type significantly shapes buyer responses.Research limitations/implicationsThis paper extends the CTT framework by considering new antecedents and outcomes. We also provide a more comprehensive understanding of buyer behavior when facing positive and negative supplier-induced disruptions. Our study has limitations. Through vignette-based behavioral experiments, there is a risk that scenarios may not accurately represent real-life situations and that decision-making dynamics could be oversimplified. Future research should incorporate nuanced measurements and conduct additional qualitative research for a comprehensive understanding.Originality/valueThis study enriches the understanding of the buyer-supplier relationship by expanding the CTT framework for a more comprehensive picture. We also offer nuanced insights into size dynamics and disruption types, emphasizing tailored strategies in supply chain management. The findings underscore the importance of understanding these nuances to employ tailored strategy in a business-to-business (B2B) context, as mediated power is contingent on multiple factors.
Analyzing the impact of country-of-origin, geographical indication and wine world on low-involvement generation Z potential consumers’ attitudes toward wine ads
Authors: Purcini Gabriela , Medeiros Barretta Leonardo Medeiros , Ferreira Luciana , Lourenção Marina 
Published: September 2024

Purpose
This study aims to compare the influence of origin types (country-of-origin – COO versus geographic indication – GI) and wine worlds (new versus old wine country – represented by Brazil and Italia, respectively) on the attitude of low-involvement Generation Z potential consumers from Brazil toward wine ads.

Design/methodology/approach
The authors conducted a two (COO vs GI) by two (New vs Old World) within-subject experimental design with 98 Brazilians from Generation Z. Latin square was used to systematically counterbalances the sequence in which participants were exposed to ads.

Findings
The results reveal that potential consumers’ attitudes are more positive when a COO is used in wine advertisements than when a GI is used. Besides, the consumers’ conative response is more positive when an Old World country (Italy) is used in wine ads than when New World country (Brazil) is used.

Research limitations/implications
This study expands existing literature by showing that, in wine ads, COO information elicits more positive responses than GI. It also suggests a preference among potential consumers for Old over New World wines in purchase, recommendation and consumption decisions.

Practical implications
The wine advertisements for the market segment investigated should highlight the COO instead of geographical indication (GI). Besides, the authors point out the importance of promoting the concept of GI among low-involvement Generation Z potential consumers.

Originality/value
To the best of the authors’ knowledge, this is the first study to assess potential consumers’ attitudes toward wine ads, comparing COO and GI and determining which geographical cue elicits more positive consumer attitudes: origin types (COO vs GI) or wine worlds (Old vs New).
Optimization of high-speed reducer in electric vehicle based on analysis of lubrication
Authors: Jia Fuchun , Liu Xianghuan , Fu Yao 
Published: September 2024

Purpose
The purposes of this paper are optimization of high speed reducer in electric vehicles based on the analysis of lubrication and verification of simulation accuracy and optimization results.

Design/methodology/approach
The traditional CFD method presents poor applicability to complex geometric problems due to grid deformity. Therefore, moving particle semi-implicit (MPS) method is applied in this study to simulate lubrication of the reducer and analyze the influence of input speed and lubrication system design on the distribution. According to the results, the reducer is optimized. Meanwhile, the experiments for lubrication and churning power loss is carried out to verify the accuracy of simulation and optimization effects.

Findings
The flow field of lubricant inside the reducer is obtained. The lubrication system of reducer needs to be improved. Simulation and experiment show that the optimization is sufficient and efficient.

Originality/value
According to the simulation of lubrication, the reducer is optimized. The lubrication experimental setup is established. The conclusion of paper can provide the method and tool for reducer in electric vehicle.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-04-2024-0123/
Excessive information on social media and Generation Z's long-term COVID-19 vaccine advocacy: a post-pandemic perspective
Authors: Liu Hongfei , Meng-Lewis Yue , Liu Wentong 
Published: September 2024

PurposeSocial media played an irreplaceable role in young people’s online social life and information consumption during the COVID-19 pandemic. This research focuses on the impact of excessive information on social media about COVID-19 vaccines on Generation Z's (Gen Z) associated psychological states and long-term vaccine advocacy.Design/methodology/approachThe research conducted structural equation modeling analysis with online survey data from 409 Gen Z citizens in the UK.FindingsThe findings suggest that excessive information increased Gen Z social media users' ambivalence and conspiracy beliefs around COVID-19 vaccines, which, in turn, reduced their long-term vaccine advocacy in terms of vaccine acceptance, vaccination intention and vaccine promotion. Importantly, Gen Z’s confidence in government and in the healthcare systems during COVID-19 was effective in helping them overcome the detrimental effects of conspiracy beliefs and ambivalence about long-term vaccine advocacy, respectively.Originality/valueThis research reveals the “dark side” of social media use in the post-pandemic period and highlights the significant roles played by social institutions in mitigating the detrimental effects of Gen Z’s support in social decisions. Beyond the context of COVID-19, this research has important implications for facilitating the civic engagement of Gen Z and boosting their confidence in social institutions in terms of social cohesion.
Modelling the conundrums to cyber-risks management in logistics firms for supply chain social sustainability
Authors: Orji Ifeyinwa Juliet , U-Dominic Chukwuebuka Martinjoe 
Published: September 2024

PurposeCybersecurity has received growing attention from academic researchers and industry practitioners as a strategy to accelerate performance gains and social sustainability. Meanwhile, firms are usually prone to cyber-risks that emanate from their supply chain partners especially third-party logistics providers (3PLs). Thus, it is crucial to implement cyber-risks management in 3PLs to achieve social sustainability in supply chains. However, these 3PLs are faced with critical difficulties which tend to hamper the consistent growth of cybersecurity. This paper aims to analyze these critical difficulties.Design/methodology/approachData were sourced from 40 managers in Nigerian 3PLs with the aid of questionnaires. A novel quantitative methodology based on the synergetic combination of interval-valued neutrosophic analytic hierarchy process (IVN-AHP) and multi-objective optimization on the basis of a ratio analysis plus the full multiplicative form (MULTIMOORA) is applied. Sensitivity analysis and comparative analysis with other decision models were conducted.FindingsBarriers were identified from published literature, finalized using experts’ inputs and classified under organizational, institutional and human (cultural values) dimensions. The results highlight the most critical dimension as human followed by organizational and institutional. Also, the results pinpointed indigenous beliefs (e.g. cyber-crime spiritualism), poor humane orientation, unavailable specific tools for managing cyber-risks and skilled workforce shortage as the most critical barriers that show the highest potential to elicit other barriers.Research limitations/implicationsBy illustrating the most significant barriers, this study will assist policy makers and industry practitioners in developing strategies in a coordinated and sequential manner to overcome these barriers and thus, achieve socially sustainable supply chains.Originality/valueThis research pioneers the use of IVN-AHP-MULTIMOORA to analyze cyber-risks management barriers in 3PLs for supply chain social sustainability in a developing nation.