bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of celiac disease and related antibodies in patients diagnosed with irritable bowel syndrome according to the Rome III criteria. A case–control study
Authors: Sánchez‐Vargas L. A. , Thomas‐Dupont P. , Torres‐Aguilera M. , Azamar‐Jacome A. A. , Ramírez‐Ceervanes K. L. , Aedo‐Garcés M. R. , Meixueiro‐Daza A. , Roesch‐Dietlen F. , Grube‐Pagola P. , Vivanco‐Cid H. , Remes‐Troche J. M. 
Published: February 2016

AbstractBackgroundThe cost‐effectiveness for screening for celiac disease (CD) in patients with irritable bowel syndrome (IBS), specifically in the diarrhea (IBS‐D) subtype, is beneficial if the prevalence is >1%. However, recent studies have shown controversial results. In this large case–control study, our aim was to determine the prevalence of CD and a panel of related antibodies in patients diagnosed with IBS.Materials and methodsFour hundred IBS patients (Rome III) and 400 asymptomatic healthy controls were prospectively evaluated using antihuman tissue transglutaminase (h‐tTG IgA) and deamidated gliadin peptide antibodies (DGP II IgA and DGP II IgG). Duodenal biopsy was performed on the patients that were positive for the h‐tTG IgA and/or DGP II IgG antibodies.ResultsThe mean age of the population was 44.47 ± 18.01 years and 335 (82%) of the subjects were women. Twenty‐one patients and six controls had at least one positive test for CD (5.25% VS 1.5%, p = 0.003, OR 3.63 [95% CI 1.4–9.11]). Eighteen patients were positive for h‐tTG and/or DGP‐II IgG. Histologic confirmation of CD was 2.5% in the IBS patients vs 0.5% in the controls (p = 0.04, OR 5.21). The IBS‐D subtype had the highest prevalence for serological positivity (12.7%).ConclusionsUp to 5.2% of the patients with IBS according to the Rome III criteria were positive for at least one of the CD‐related antibodies and 2.5% had biopsy‐confirmed CD. Therefore, in our population, screening for CD in subjects with IBS appears to be a reasonable strategy, especially in the IBS‐D subgroup.
Nitric oxide and S‐nitrosoglutathione function additively during plant immunity
Authors: Yun Byung‐Wook , Skelly Michael J. , Yin Minghui , Yu Manda , Mun Bong‐Gyu , Lee Sang‐Uk , Hussain Adil , Spoel Steven H. , Loake Gary J. 
Published: February 2016

Summary

Nitric oxide (NO) is emerging as a key regulator of diverse plant cellular processes. A major route for the transfer of NO bioactivity is S‐nitrosylation, the addition of an NO moiety to a protein cysteine thiol forming an S‐nitrosothiol (SNO). Total cellular levels of protein S‐nitrosylation are controlled predominantly by S‐nitrosoglutathione reductase 1 (GSNOR1) which turns over the natural NO donor, S‐nitrosoglutathione (GSNO). In the absence of GSNOR1 function, GSNO accumulates, leading to dysregulation of total cellular S‐nitrosylation.

Here we show that endogenous NO accumulation in Arabidopsis, resulting from loss‐of‐function mutations in NO Overexpression 1 (NOX1), led to disabled Resistance (R) gene‐mediated protection, basal resistance and defence against nonadapted pathogens. In nox1 plants both salicylic acid (SA) synthesis and signalling were suppressed, reducing SA‐dependent defence gene expression.

Significantly, expression of a GSNOR1 transgene complemented the SNO‐dependent phenotypes of paraquat resistant 2‐1 (par2‐1) plants but not the NO‐related characters of the nox1‐1 line. Furthermore, atgsnor1‐3 nox1‐1 double mutants supported greater bacterial titres than either of the corresponding single mutants.

Our findings imply that GSNO and NO, two pivotal redox signalling molecules, exhibit additive functions and, by extension, may have distinct or overlapping molecular targets during both immunity and development.
In vivo photodynamic inactivation of Candida albicans using chloro‐aluminum phthalocyanine
Authors: Carmello JC , Alves F , Ribeiro APD , Basso FG , de Souza Costa CA , Tedesco AC , Primo FL , Mima EG , Pavarina AC 
Published: February 2016

This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro‐aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro‐aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy‐five 6‐week‐old female Swiss mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100 J cm−2–660 nm). Twenty‐four hours and 7 days after treatments, microbiological evaluation was carried out by recovering C. albicans from the tongue of animals (CFU ml−1). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro‐ and anti‐inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP‐NE‐mediated PDT reduced 2.26 log10 of C. albicans recovered from the tongue when compared with the control group (P−L−) (P < 0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro‐aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C. albicans recovered from the oral lesions of immunocompromised mice.
Pharmacological targeting of histamine H4 receptor in periodontal disease
Authors: Prestifilippo JP , Fernández‐Solari J , Martinel Lamas DJ , Rios CE , Mohn C , Perazzo JC , Rivera ES , Elverdin JC , Medina VA 
Published: February 2016

ObjectiveThe objective of this study was to investigate whether histamine H4 receptor (H4R) antagonists could prevent experimental periodontitis (EP)‐induced histological, functional and inflammatory alterations in submandibular gland (SMG), periodontal bone and gingiva.MethodsBilateral EP was induced for 2 weeks in anaesthetized male rats. The effect of systemic and local administration of H4R antagonists (JNJ7777120, JNJ10191584) on histopathology and functionality of SMG, bone loss and gingival inflammation was evaluated.ResultsThe subcutaneous administration of JNJ7777120 prevented periodontitis‐induced SMG histological injury, reducing vacuolization and apoptosis and additionally reversed the increased prostaglandin E2 (PGE2) levels in SMG while it partially reversed the methacholine‐induced salivation reduction produced by periodontitis. JNJ7777120 attenuated bone loss and the increased PGE2 levels and inflammatory infiltration in gingival tissue of rats with periodontitis. Finally, local administration of JNJ7777120 and JNJ10191584 was also beneficial for improving periodontal parameters.ConclusionsH4 receptor antagonists are able to ameliorate periodontitis‐induced injury on SMG, gingival tissue and bone structure, suggesting that pharmacological targeting of H4R could be an attractive strategy to improve periodontal health.
Health education programmes to improve foot self‐care practices and foot problems among older people with diabetes: a systematic review
Authors: Ahmad Sharoni Siti Khuzaimah , Minhat Halimatus Sakdiah , Mohd Zulkefli Nor Afiah , Baharom Anisah 
Published: February 2016

Aims and objectivesTo assess the effectiveness of health education programmes to improve foot self‐care practices and foot problems among older people with diabetes.BackgroundThe complications of diabetes among older people are a major health concern. Foot problems such as neuropathy, ulcer and ultimately amputation are a great burden on older people with diabetes. Diabetes foot education programmes can influence the behaviour of older people in practising foot self‐care and controlling the foot problems. However, the educational approaches used by the educators are different. Therefore, it is important to assess the education programmes from various evidence‐based practices.DesignSix databases, EBSCOhost medical collections (MEDLINE, CINAHL, Psychology and Behavioral Sciences Collection), SAGE, Wiley Online Library, ScienceDirect, SpringerLink and Web of Science, were used to search for articles published from January 2000 to March 2015. The search was based on the inclusion criteria and keywords including ‘foot’, ‘care’ and ‘diabetes’. Fourteen studies were assessed and reviewed in the final stage.ConclusionsHealth education programmes varied according to their design, setting, approach, outcome measured and results. Foot assessment, verbal and written instructions and discussion were proved to improve the foot self‐care and foot problems. Subsequent follow‐ups and evaluations had a significant effect. An improvement was observed in foot self‐care scores and foot problems (such as neuropathy, foot disability, lesion, ulcer, tinea pedis and callus grade) after implementation of the health education programme.Implications for practiceThe findings of this study support the claim that a health education programme increases the foot self‐care scores and reduces the foot problems. However, there were certain methodological concerns in the reviewed articles, indicating the need for further evaluation. In future, researchers and practitioners must implement a vigorous education programme focusing on diabetes foot self‐care among the older population.
Abnormal Pain Response to Visual Feedback During Cervical Movements in Chronic Whiplash: An Experimental Study
Authors: De Kooning Margot , Daenen Liesbeth , Verhelpen Sam , Don Sanneke , Voogt Lennard , Roussel Nathalie , Ickmans Kelly , Van Loo Michel , Cras Patrick , Nijs Jo 
Published: February 2016

AbstractBackgroundWhiplash‐associated disorders (WAD) are a debilitating condition. In chronic WAD, sensorimotor incongruence exacerbates symptoms. Sensorimotor incongruence occurs when somatosensory input and predicted motor output are in conflict, which can trigger pain. On the other hand, there is evidence that visual feedback can decrease pain in certain chronic pain conditions. Therefore, the aim of this study was to examine the effect of visual feedback and sensorimotor incongruence on pain thresholds in chronic WAD.MethodsSixty‐four participants (healthy controls and patients with chronic WAD) were subjected to six experimental conditions. Participants watched correct real‐time or modified visual feedback of the neck or hand (without movement as well as during repetitive neck lateroflexion). Sensorimotor incongruence was induced by manipulating visual feedback. Pressure pain thresholds were measured at baseline and during each condition.ResultsMarked between‐group differences were observed. Visual feedback of the neck—correct or modified—did not influence pain thresholds in chronic WAD. In contrast, healthy controls had significantly higher pain thresholds when provided with the correct or modified visual feedback. When a movement of the neck was added during visual feedback, patients with chronic WAD showed no significant difference in pain thresholds, while an increase in pain thresholds was found in the healthy control group.ConclusionIn contrast to the healthy controls, visual feedback and sensorimotor incongruence did not alter pain thresholds in patients with chronic WAD. These findings suggest an abnormal pain response to visual feedback and somatosensory incongruence as well as failing mechanisms of pain inhibition in chronic WAD.
Fast‐forward genetics by radiation hybrids to saturate the locus regulating nuclear–cytoplasmic compatibility in Triticum
Authors: Bassi Filippo M. , Ghavami Farhad , Hayden Matthew J. , Wang Yi , Forrest Kerrie L. , Kong Stephan , Dizon Rhoderissa , Michalak de Jimenez Monika K. , Meinhardt Steven W. , Mergoum Mohamed , Gu Yong Q. , Kianian Shahryar F. 
Published: February 2016

SummaryThe nuclear‐encoded species cytoplasm specific (scs) genes control nuclear–cytoplasmic compatibility in wheat (genus Triticum). Alloplasmic cells, which have nucleus and cytoplasm derived from different species, produce vigorous and vital organisms only when the correct version of scs is present in their nucleus. In this study, bulks of in vivo radiation hybrids segregating for the scs phenotype have been genotyped by sequencing with over 1.9 million markers. The high marker saturation obtained for a critical region of chromosome 1D allowed identification of 3318 reads that mapped in close proximity of the scs. A novel in silico approach was deployed to extend these short reads to sequences of up to 70 Kb in length and identify candidate open reading frames (ORFs). Markers were developed to anchor the short contigs containing ORFs to a radiation hybrid map of 650 individuals with resolution of 288 Kb. The region containing the scs locus was narrowed to a single Bacterial Artificial Chromosome (BAC) contig of Aegilops tauschii. Its sequencing and assembly by nano‐mapping allowed rapid identification of a rhomboid gene as the only ORF existing within the refined scs locus. Resequencing of this gene from multiple germplasm sources identified a single nucleotide mutation, which gives rise to a functional amino acid change. Gene expression characterization revealed that an active copy of this rhomboid exists on all homoeologous chromosomes of wheat, and depending on the specific cytoplasm each copy is preferentially expressed. Therefore, a new methodology was applied to unique genetic stocks to rapidly identify a strong candidate gene for the control of nuclear–cytoplasmic compatibility in wheat.
Evolution of risk assessment strategies for food and feed uses of stacked GM events
Authors: Kramer Catherine , Brune Phil , McDonald Justin , Nesbitt Monique , Sauve Alaina , Storck‐Weyhermueller Sabine 
Published: February 2016

SummaryData requirements are not harmonized globally for the regulation of food and feed derived from stacked genetically modified (GM) events, produced by combining individual GM events through conventional breeding. The data required by some regulatory agencies have increased despite the absence of substantiated adverse effects to animals or humans from the consumption of GM crops. Data from studies conducted over a 15‐year period for several stacked GM event maize (Zea mays L.) products (Bt11 × GA21, Bt11 × MIR604, MIR604 × GA21, Bt11 × MIR604 × GA21, Bt11 × MIR162 × GA21 and Bt11 × MIR604 × MIR162 × GA21), together with their component single events, are presented. These data provide evidence that no substantial changes in composition, protein expression or insert stability have occurred after combining the single events through conventional breeding. An alternative food and feed risk assessment strategy for stacked GM events is suggested based on a problem formulation approach that utilizes (i) the outcome of the single event risk assessments, and (ii) the potential for interactions in the stack, based on an understanding of the mode of action of the transgenes and their products.
The secretion of the bacterial phytase PHY‐US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co‐growth
Authors: Belgaroui Nibras , Berthomieu Pierre , Rouached Hatem , Hanin Moez 
Published: February 2016

SummaryPhytic acid (PA) is a major source of inorganic phosphate (Pi) in the soil; however, the plant lacks the capacity to utilize it for Pi nutrition and growth. Microbial phytases constitute a group of enzymes that are able to remobilize Pi from PA. Thus, the use of these phytases to increase the capacity of higher plants to remobilize Pi from PA is of agronomical interest. In the current study, we generate transgenic Arabidopsis lines (ePHY) overexpressing an extracellular form of the phytase PHY‐US417 of Bacillus subtilis, which are characterized by high levels of secreted phytase activity. In the presence of PA as sole source of Pi, while the wild‐type plants show hallmark of Pi deficiency phenotypes, including the induction of the expression of Pi starvation‐induced genes (PSI, e.g. PHT1;4) and the inhibition of growth capacity, the ePHY overexpressing lines show a higher biomass production and no PSI induction. Interestingly, when co‐cultured with ePHY overexpressors, wild‐type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production. In line with these results, mutants in the high‐affinity Pi transporters, namely pht1;1 and pht1;1‐1;4, both fail to accumulate Pi and to grow when co‐cultured with ePHY overexpressors. Taken together, these data demonstrate the potential of secreted phytases in improving the Pi content and enhancing growth of not only the transgenic lines but also the neighbouring plants.
Targeting insect mitochondrial complex I for plant protection
Authors: Wu Xiu‐Ming , Yang Chang‐Qing , Mao Ying‐Bo , Wang Ling‐Jian , Shangguan Xiao‐Xia , Chen Xiao‐Ya 
Published: February 2016

SummaryPlant engineered to express double‐stranded RNA (dsRNA) can target the herbivorous insect gene for silencing. Although mounting evidence has emerged to support feasibility of this new pest control technology, field application is slow largely due to lack of potent targets. Here, we show that suppression of the gene encoding NDUFV2, a subunit of mitochondrial complex I that catalyses NADH dehydrogenation in respiratory chain, was highly lethal to insects. Feeding cotton bollworm (Helicoverpa armigera) larvae with transgenic cotton tissues expressing NDUFV2 dsRNA led to mortality up to 80% within 5 days, and almost no larvae survived after 7 days of feeding, due to the altered mitochondrial structure and activity. Transcriptome comparisons showed a drastic repression of dopa decarboxylase genes. Reciprocal assays with Asian corn borer (Ostrinia furnacalis), another lepidopteran species, revealed the sequence‐specific effect of NDUFV2 suppression. Furthermore, the hemipteran lugus Apolygus lucorum was also liable to NDUFV2 repression. These data demonstrate that the mitochondrial complex I is a promising target with both sequence specificity and wide applicability for the development of new‐generation insect‐proof crops.
Recommendations from a European Roundtable Meeting on Best Practice Healthy Infant Skin Care
Authors: Blume‐Peytavi Ulrike , Lavender Tina , Jenerowicz Dorota , Ryumina Irina , Stalder Jean‐Francois , Torrelo Antonio , Cork Michael J. 
Published: February 2016

AbstractBackgroundEuropean roundtable meeting recommendations on bathing and cleansing of infants were published in 2009; a second meeting was held to update and expand these recommendations in light of new evidence and the continued need to address uncertainty surrounding this aspect of routine care.MethodsThe previous roundtable recommendations concerning infant cleansing, bathing, and use of liquid cleansers were critically reviewed and updated and the quality of evidence was evaluated using the Grading of Recommendation Assessment, Development and Evaluation system. New recommendations were developed to provide guidance on diaper care and the use of emollients. A series of recommendations was formulated to characterize the attributes of ideal liquid cleansers, wipes, and emollients.ResultsNewborn bathing can be performed without harming the infant, provided basic safety procedures are followed. Water alone or appropriately designed liquid cleansers can be used during bathing without impairing the skin maturation process. The diaper area should be kept clean and dry; from birth, the diaper area may be gently cleansed with cotton balls/squares and water or by using appropriately designed wipes. Appropriately formulated emollients can be used to maintain and enhance skin barrier function. Appropriately formulated baby oils can be applied for physiologic (transitory) skin dryness and in small quantities to the bath. Baby products that are left on should be formulated to buffer and maintain babies’ skin surface at approximatelypH5.5, and the formulations and their constituent ingredients should have undergone an extensive program of safety testing. Formulations should be effectively preserved; products containing harsh surfactants, such as sodium lauryl sulfate, should be avoided.ConclusionHealth care professionals can use these recommendations as the basis of their advice to parents.
Orthotopic liver transplant for multifocal lymphangioendotheliomatosis with thrombocytopenia
Authors: Yang Christine H. , Zhou Shengmei , Alexopoulos Sophoclis , Wang Larry , Baron Howard I. , Genyk Yuri , Kerkar Nanda 
Published: February 2016

AbstractAn eight‐yr‐old female with a history of multifocal lymphangioendotheliomatosis and thrombocytopenia presented for MVT. The patient had multiple vascular lesions in the skin and stomach in infancy. Although her cutaneous lesions resolved with vincristine and methylprednisolone, her gastric lesions persisted. Eight yr later, she was diagnosed with portal hypertension and decompensating liver function despite therapy with bevacizumab, propranolol, furosemide, and spironolactone. Upon presentation, she was found to have a Kasabach–Merritt‐like coagulopathy in association with multiple lesions in her GI tract and persistent gastric lesions. Although treatment with methylprednisolone and sirolimus normalized her coagulation factors and d‐dimer levels, she never developed sustained improvement in her thrombocytopenia. Her liver function continued to deteriorate and she developed hepatorenal syndrome. Given better outcomes after OLT in comparison with MVT, she underwent OLT, with the plan to manage her GI lesions with APC post‐transplant. Post‐transplant, her liver function and coagulopathy normalized, and GI tract lesions disappeared upon screening with capsule endoscopy. The patient is doing well, without recurrence of either GI lesions or thrombocytopenia, at 18 months after transplantation.
Application of the Extended Health Control Belief Model to Predict Hepatitis A and B Vaccinations
Authors: Reynolds Grace L. , Nguyen Hannah H. , Singh‐Carlson Savitri , Fisher Dennis G. , Odell Anne , Xandre Pamela 
Published: February 2016

AbstractBackgroundAdult vaccination compliance rates vary according to sample and type of vaccine administered (influenza, pneumococcal). This study looked at vaccination of a community sample of low‐income, minority adults.MethodsNurses offered free vaccination for hepatitis A and B in the form of the combined Twinrix vaccine to adults on a walk‐in basis. In addition to dosing information, participants completed the Risk Behavior Assessment, the Coping Strategies Indicator and the Cardiovascular Risk Assessment. Skaff's extended Health Belief Model was used as the theoretical framework. Count regression was used to model receipt of one, two, or three doses.ResultsThe majority of participants were male with a mean age of 40 years. The distribution of doses was: 173 individuals (27.6%) received one dose only, 261 (41.7%) received two doses, and 191 (30.5%) received three doses of vaccine. The multivariate count regression model including being male, having previously been told by a health care provider that one has syphilis, having severe negative emotions, and perceived social support were associated with participants' receiving fewer doses of hepatitis vaccine. A greater problem‐solving score was associated with a higher number of vaccine doses received.ConclusionDespite free vaccinations offered in an easily accessible community setting, the majority of participants failed to complete the hepatitis vaccine series. More effort is needed to get adult men to participate in hepatitis vaccination clinics. Additional research is necessary to understand barriers other than cost to adults receiving vaccination.