bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Production of Bacterial Cellulose Using Komagataeibacter sucrofermentans on Sustainable Feedstocks
Authors: Liu Ziyao , Siddique Faiza , Wei Yan , Haque Md. Ariful , Na Li , Yang Xiaofeng , Lin Carol Sze Ki 
Published: October 2024

AbstractThe production of bacterial cellulose (BC) has indeed garnered global attention due to its versatile properties and applications. Despite potential benefits, the challenges like low productivity, high fermentation costs, and expensive culture media hinder its industrialization. Utilizing low‐cost substrates, especially waste streams, can help address the challenges. In this study, waste feedstocks such as restaurant leftovers, oranges, and grapefruit from canteens and supermarkets were valorized for BC production by Komagataeibacter sucrofermentans. Orange juice is a fascinating substrate with a highest concentration of 20.6 g/L and productivity of 2.05 g/L/d. Using HS medium with supplementary ions, organic acids, ethanol, and various carbon sources is a strategic approach for enhancing BC production. The study reveals that the addition of organic acids or ethanol moderately increased BC production, while ions inhibit BC synthesis, highlighting the complex interplay between various cultivation medium components. Additionally, fermentation with K. sucrofermentans using single and mixed carbon sources was conducted to elucidate the potential metabolic mechanism of BC production. Through alkaline treatment and drying in a 30 °C incubator, we produced the highest quality BC with 92.09 % crystallinity. Overall, the study enhances BC production knowledge and provides green and sustainable strategies for fermentative BC production.
New Redox Chemistries of Halogens in Aqueous Batteries
Authors: Chen Ruiyong 
Published: October 2024

AbstractHalogen‐based redox‐active materials represent an important class of materials in aqueous electrochemistry. The existence of versatile halogen species and their rich bonding coordination create great flexibility in designing new redox couples. Novel redox reaction mechanisms and electrochemical reversibility can be unlocked in specifically configurated electrolyte environments and electrodes. In this review, the halogen‐based redox couples and their appealing redox chemistries in aqueous batteries, including redox flow batteries and traditional static batteries that have been studied in recent years, are discussed. New aqueous electrochemistry provides hope to outperform the state‐of‐the‐art materials and systems that are facing resources and performance limitation, and to enrich the existing battery chemistries.
The outcomes of endoscopic ultrasound‐guided tissue acquisition for small focal liver lesions measuring ≤2 cm
Authors: Takano Yuichi , Tamai Naoki , Yamawaki Masataka , Noda Jun , Azami Tetsushi , Niiya Fumitaka , Nishimoto Fumiya , Maruoka Naotaka , Yamagami Tatsuya , Nagahama Masatsugu 
Published: October 2024

AbstractObjectivesEndoscopic ultrasound‐guided tissue acquisition (EUS‐TA) for focal liver lesions has gained attention as an alternative to percutaneous biopsy. Although the outcomes of EUS‐TA for focal liver lesions have been reported to be favorable, no studies have focused on small focal liver lesions (≤2 cm). The aim of this study was to evaluate the outcomes of EUS‐TA for small focal liver lesions (≤2 cm).MethodsThe details of EUS‐TA performed for focal liver lesions between 2016 and 2022 were retrospectively reviewed. The outcomes were compared between cases involving ≤2 cm lesions and those involving >2 cm lesions. The primary outcomes were diagnostic ability and adverse events.ResultsEUS‐TA for focal liver lesions was performed in 109 cases. Of the 109 cases, 32 (29.3%) involved ≤2 cm lesions and 77 (70.6%) involved >2 cm lesions. Right lobe lesions and transduodenal puncture were significantly fewer in the ≤2 cm group. There were no significant differences in needle gauge, needle type, or number of punctures between the groups. The sensitivity, specificity, and accuracy rates were 96.8%, 100%, and 96.8%, respectively, in the ≤2 cm group and 97.4%, 100%, and 97.4%, respectively, in the >2 cm group, with no significant differences between the groups. There was no difference in adverse events between the groups (0% in the ≤2 cm group and 2.3% in the >2 cm group).ConclusionsEUS‐TA for small focal liver lesions measuring ≤2 cm has favorable outcomes, which are similar to those for lesions measuring >2 cm.
Population monitoring and conservation implications of intra‐ and interspecific nest occupation rates in swallows
Authors: Tella José L. , Sánchez‐Prieto Cristina B. , Romero‐Vidal Pedro , Serrano David , Blanco Guillermo 
Published: October 2024

AbstractWith the exception of a few groups of birds, such as large raptors and colonial seabirds, direct counts of nests cannot be conducted over very large areas for most of the abundant and widely distributed species, and thus indirect methods are used to estimate their relative abundances and population sizes. However, many species of the Family Hirundinidae (swallows and martins) build their mud nests in discrete, predictable and accessible sites, which are reused across years. Therefore, the direct count of active nests could constitute a reliable method for estimating breeding population sizes and their changes at large spatial and temporal scales. We illustrate the feasibility of this monitoring approach through a single year survey of >2700 nests of three coexisting Old‐World species, the barn swallow (Hirundo rustica), the red‐rumped swallow (Cecropis daurica), and the crag martin (Ptyonoprogne rupestris), distributed across Portugal and Spain. Our results revealed changes in the use of nesting substrates and increases in interspecific nest usurpation rates over recent decades. While 56% of the nests of C. daurica were located in rocks five decades ago, almost 100% are nowadays located in anthropogenic substrates such as bridges, road culverts, and abandoned buildings, which could have favored the range expansion of this species. Nest occupation rates were surprisingly low (12% in C. daurica, 21% in H. rustica, and 37% in P. rupestris), and the proportion of abandoned nesting sites was very high (65% in C. daurica, 50% in H. rustica, and 27% in P. rupestris). Abandonment rates reflect the population decline reported for H. rustica. Notably, the usurpation of nests of C. daurica by house sparrows Passer domesticus, which is the main cause of breeding failure, has increased from 2.4% in 1976–1979 to 34.7% of the nests nowadays. The long‐term monitoring of nests may constitute a reliable and affordable method, with the help of citizen science, for assessing changes in breeding population sizes and conservation threats of these and other mud‐nest building hirundines worldwide.
Seasonal variations in composition and function of gut microbiota in grazing yaks: Implications for adaptation to dietary shift on the Qinghai‐Tibet plateau
Authors: Wang Xungang , Guo Tongqing , Zhang Qian , Zhao Na , Hu Linyong , Liu Hongjin , Xu Shixiao 
Published: October 2024

AbstractGut microbiome of animals is affected by external environmental factors and can assist them in adapting to changing environments effectively. Consequently, elucidating the gut microbes of animals under different environmental conditions can provide a comprehensive understanding of the mechanisms of their adaptations to environmental change, with a particular focus on animals in extreme environments. In this study, we compared the structural and functional differences of the gut microbiome of grazing yaks between the summer and winter seasons through metagenomic sequencing and bioinformatics analysis. The results indicated that the composition and function of microbes changed significantly. The study demonstrated an increase in the relative abundance of Actinobacteria and a higher ratio of Firmicutes to Bacteroidetes (F/B) in winter, this process facilitated the adaptation of yaks to the consumption of low‐nutrient forages in the winter. Furthermore, the network structure exhibited greater complexity in the winter. Forage nutrition exhibited a significant seasonal variation, with a notable impact on the gut microbiota. The metagenomic analysis revealed an increase in the abundance of enzymes related to amino acid metabolism, axillary activity, and mucin degradation in the winter. In conclusion, this study demonstrated that the gut microbiome of grazing yaks exhibits several adaptive characteristics that facilitate better nutrient accessibility and acid the host in acclimating to the harsh winter conditions. Furthermore, our study offers novel insights into the mechanisms of highland animal adaptation to external environments from the perspective of the gut microbiome.
Phenotypic and Transcriptomic Analysis Revealed a Lack of Risk Perception by Native Tadpoles Toward Novel Non‐Native Fish
Authors: Wang Yuanfei , Zhu Yudong , He Liuyang , Yu Haoqi , Lin Xiuqin , Ran Jianghong , Xie Feng 
Published: October 2024

ABSTRACTThe introduction of alien species poses a serious threat to native biodiversity, and mountain lake systems in the southwest of China are particularly vulnerable to the introduction of non‐native fish. The prey naivety hypothesis states that native species may not be able to recognize novel introduced species due to a lack of common evolutionary background and therefore become easy targets, so the impacts of non‐native fish on mountain endemic amphibians need to be urgently assessed. In an ex‐situ experiment, we exposed the tadpoles of the Chaochiao Brown Frog (Rana chaochiaoensis), endemic to western China, to kairomones of both native and translocated fish species, and their phenotypic and genetic response patterns were compared. The results revealed significant phenotypic plasticity responses in total length (TOL), tail length (TL), and tail muscle width (TW) of tadpoles induced by native fish kairomone, while tadpoles exposed to translocated fish kairomone exhibited weaker phenotypic changes. At the transcriptional level, the number of differently expressed genes (DEGs) in the native fish treatment was 3.1‐fold (liver) and 52.6‐fold (tail muscle) higher than in the translocated fish treatment, respectively. There were more unique DEGs in the native fish treatment, primarily enriched in terms and pathways related to stress response, energy metabolism, and muscle development. The study revealed a lack of risk perception by native tadpoles toward novel non‐native fish, providing new evidence for the prey naivety hypothesis from both phenotypic and molecular perspectives. Future conservation efforts should prioritize assessing the impacts of non‐native fish on alpine and subalpine threatened and narrowly distributed amphibians. Additionally, prevention, early warning, monitoring, and removal of non‐native fish should be carried out as soon as possible.
Regioselective Synthesis of Thiazole‐5‐thione Based on [2+3] Annulation Trapping of α‐Keto Sulfine with Thioamide
Authors: Zhang Xuehua , Lin Yingxue , Guo Mingyang , Xu Kewen , Zhu Jinhui , Dong Jun 
Published: October 2024

AbstractAiming to develop novel annulation reactions using sulfine intermediates, we have established a regioselective formal [2+3] cyclization reaction of α‐keto sulfines generated from the elimination of arylacyl sulfoxides and thioamides. This new strategy allows for the synthesis of thiazole‐5‐thiones directly without catalysts or additives. The approach features a step‐economic, one‐pot characteristics via a tandem sequence of in situ α‐keto sulfines generation, regiospecifically nucleophilic attack or [3+2] cyclizatoin, dithioate (S=C−S−) skeletons formation, and subsequent intramolecular nucleophilic cyclization. This reaction also represents a novel method for constructing dithioate (S=C−S−) skeletons.
Pyrolysis models for pressure treated wood and wood–plastic composite
Authors: Lee Sangkyu , De Beer Jacques A. , Stoliarov Stanislav I. , Sunderland Peter B. , Gollner Michael J. 
Published: October 2024

AbstractPressure treated wood (PTW) and wood–plastic composites such as Trex® are popular materials for the construction of decks and other auxiliary structures, which are known to significantly contribute to spread of wildland fires into communities. In this work, representative samples of these materials were studied to determine their pyrolysis and combustion properties to enable simulation of fire growth on the surface of these building products. The pyrolysis property development process followed a well‐established hierarchical approach where thermogravimetric analysis, differential scanning calorimetry, and microscale combustion calorimetry were used to parametrize kinetics and thermodynamics of the thermal decomposition and combustion, while controlled atmosphere pyrolysis and cone calorimetry tests performed on coupon‐sized samples were used to parameterize thermal transport properties and validate performance of the fully parametrized pyrolysis models. PTW decomposition was captured using four sequential reactions with one additional reaction used to model vaporization of water. Trex® board was found to consist of two distinct layers: a thin outer layer and an internal core. The pyrolysis model for this material was constructed using some known properties of high‐density polyethylene (PE) and the properties of PTW determined in this work. The outer layer was defined in the model to consist of PE and an inert additive, while the core was defined as a blend of PE and wood particles, which kinetics and thermodynamics of the thermal decomposition and combustion were successfully captured using the model developed for PTW.
Berry pomace as a potential ingredient for plant‐based meat analogs
Authors: Zhang Jingnan , Li Yiyao 
Published: October 2024

AbstractGiven the projected global population growth and the associated increase in demand for sustainable and nutritious food options, plant‐based meat analogs are increasingly popular. Berry pomace, a by‐product of the juice and wine industry, emerges as a promising ingredient for enhancing these products. This paper comprehensively explores the innovative use of berry pomace in the development of plant‐based meat analogs. It highlights key components such as antioxidants, natural colorants, dietary fibers, oils, and micronutrients, which significantly contribute to enhancing the nutritional profiles, sensory qualities, and shelf stability of these analogs. Methods for incorporating berry pomace into plant‐based meats, including direct addition and the addition of berry pomace extract using innovative technologies, such as high‐moisture extrusion, 3D printing and emulsion methods, are discussed. Moreover, the challenges of integrating berry pomace into plant‐based meats are critically analyzed, focusing on variability in pomace composition, potential sensory impact, and the technological adaptations required for optimal use in food production. The potential of berry pomace to enhance both the quality and appeal of plant‐based meats is highlighted, underscoring its significant contribution to the development of more sustainable food systems by valorizing food waste to high‐value products.
Exploring Consumption of Ultra‐Processed Foods and Diet Quality in the Context of Popular Low Carbohydrate and Plant‐Based Dietary Approaches
Authors: De La Motte Kayla‐Anne Lenferna , Campbell Jessica L. , Zinn Caryn 
Published: October 2024

ABSTRACTThis study investigates diet quality across four popular dietary patterns: Ketogenic Diet, Low‐Carbohydrate Healthy‐Fat, Vegetarian, and Vegan, employing the NOVA and Human Interference Scoring System (HISS) classification systems. Utilizing a modified Food Frequency Questionnaire (FFQ) and analyzing 168 participants' dietary habits, the research identifies notable differences in dietary quality among the dietary patterns. While all groups reported lower consumption of UPFs than the general population, plant‐based diets demonstrated higher UPF consumption than ketogenic and low carbohydrate diets. The study reveals that both NOVA and HISS effectively identify UPFs, with significant differences observed at various processing levels, except for UPFs where both systems showed similarity. This research contributes to the detailed understanding of diet quality within popular dietary patterns, highlighting the importance of considering food processing in dietary choices and the need for ongoing research to further elucidate the health implications of different types of UPFs.
Uptake of alpha‐synuclein preformed fibrils is suppressed by inflammation and induces an aberrant phenotype in human microglia
Authors: Niskanen Jonna , Peltonen Sanni , Ohtonen Sohvi , Fazaludeen Mohammad Feroze , Luk Kelvin C. , Giudice Luca , Koistinaho Jari , Malm Tarja , Goldsteins Gundars , Albert Katrina , Lehtonen Šárka 
Published: October 2024

AbstractMicroglia are brain resident immune cells that maintain proteostasis and cellular homeostasis. Recent findings suggest that microglia dysfunction could contribute to the pathogenesis of Parkinson's disease (PD). One of the hallmarks of PD is the aggregation and accumulation of alpha‐synuclein (αSyn) into Lewy bodies inside nerve cells. Microglia may worsen the neuronal microenvironment by persistent inflammation, resulting in deficient clearing of aggregated αSyn. To model microglial behavior in PD, we utilized human induced pluripotent stem cells to generate functionally active microglia. We studied the microglial uptake of alpha‐synuclein preformed fibrils (PFFs) and the effect of pro‐inflammatory stimulation by interferon gamma. We demonstrate that combined exposure disrupts the phagosome maturation pathway while inflammatory stimuli suppress chaperone mediated autophagy and mitochondrial function. Furthermore, inflammatory stimulation impairs PFF uptake in microglia and increases cytokine production. Moreover, excessive PFF uptake by microglia results in induction of inducible nitric oxide synthase. Taken together, we demonstrate that this model is valuable for investigating the behavior of microglia in PD and provide new insights on how human microglia process aggregated αSyn.
Long‐term outcome of 2‐year survivors after allogeneic hematopoietic cell transplantation for acute leukemia
Authors: Larue Marion , Labopin Myriam , Schroeder Thomas , Huang Xiao‐jun , Blau Igor W. , Schetelig Johannes , Ganser Arnold , Hamladji Rose‐Marie , Bethge Wolfgang , Kröger Nicolaus , Socié Gerard , Salmenniemi Urpu , Sengeloev Henrik , Dholaria Bhagirathbhai , Savani Bipin N. , Nagler Arnon , Ciceri Fabio , Mohty Mohamad 
Published: October 2024

AbstractInformation on late complications in patients with acute leukemia who have undergone allogeneic hematopoietic cell transplantation (HCT) is limited. We performed a left‐truncated analysis of long‐term survival in patients with acute leukemia who were alive and disease‐free 2 years after HCT. We included 2701 patients with acute lymphoblastic leukemia (ALL) and 9027 patients with acute myeloid leukemia (AML) who underwent HCT between 2005 and 2012. The 10‐year overall survival (OS) rate was 81.3% for ALL and 76.2% for AML, with the main causes of late mortality being relapse (ALL‐33.9%, AML‐44.9%) and chronic graft‐versus‐host disease (ALL‐29%, AML‐18%). At 10 years, HCT‐related mortality was 16.8% and 20.4%, respectively. Older age and unrelated donor transplantation were associated with a worse prognosis for both types of leukemia. In addition, transplantation in the second or third complete remission and peripheral blood HSC for ALL are associated with worse outcomes. Similarly, adverse cytogenetics, female donor to male patient combination, and reduced intensity conditioning in AML contribute to poor prognosis. We conclude that 2‐year survival in remission after HCT for acute leukemia is encouraging, with OS of nearly 80% at 10 years. However, the long‐term mortality risk of HCT survivors remains significantly higher than that of the age‐matched general population. These findings underscore the importance of tailoring transplantation strategies to improve long‐term outcomes in patients with acute leukemia undergoing HCT.
Vogt‐Koyanagi‐Harada disease‐like uveitis induced by nivolumab in metastatic renal cell carcinoma
Authors: Imai Hitomi , Koguchi Tomoyuki , Harigane Yuki , Yaginuma Kei , Meguro Satoru , Hoshi Seiji , Hata Junya , Akaihata Hidenori , Ogawa Soichiro , Kojima Yoshiyuki 
Published: October 2024

IntroductionNivolumab can cause various immune‐related adverse events; it rarely induces Vogt‐Koyanagi‐Harada‐disease‐like uveitis. Vogt‐Koyanagi‐Harada‐disease is reported to be closely associated with human leukocyte antigen‐DR4.Case presentationA 68‐year‐old man with metastatic renal cancer underwent nephrectomy. Computed tomography showed multiple lung tumors, raising suspicion of lung metastases. However, one lung hilar mass was suspected to be primary lung cancer, leading to a lobectomy, which subsequently revealed lung metastases of renal cancer. The patient underwent nivolumab treatment but developed Vogt‐Koyanagi‐Harada‐disease‐like uveitis as an immune‐related adverse event. Human leukocyte antigen‐DR4 alleles were identified, and the uveitis improved with topical steroids. He maintained partial response of lung metastases after nivolumab resumption. Immunohistochemical staining revealed significantly higher human leukocyte antigen‐DR4 expression in lung metastasis than primary renal cancer.ConclusionDespite inducing Vogt‐Koyanagi‐Harada‐disease‐like uveitis, nivolumab controlled cancer progression effectively. Immunohistochemical staining results suggest the potential involvement of human leukocyte antigen‐DR4 expression in both the onset of Vogt‐Koyanagi‐Harada‐disease‐like uveitis and nivolumab efficacy.
Adapting cybersecurity maturity models for resource‐constrained settings: A case study of Peru
Authors: Lee GangSeok , Kim SuHyun , Lee ImYoung , Brown Suzana , Carbajal Yuri Aldoradin 
Published: October 2024

AbstractDeveloping countries are rapidly embracing digitalization, but this exposes them to heightened cybersecurity risks. They often look to standard established cybersecurity models from developed countries to build their national defenses. However, significant developmental, political, social, and economic differences can render these models unsuitable for developing countries. This study addresses this gap by proposing a new framework that would be more useful in a developing country context. We first examine existing cybersecurity maturity models (CMMs) and metrics. Through a case study of Peru's national computer security incident response team (CSIRT), we assess the applicability of the security incident management maturity model (SIM3) and the security operation center CMM (SOC‐CMM) frameworks. By applying these frameworks to the Peruvian context, we identify limitations in standard maturity models for developing countries. In response, we propose a novel framework that allows developing countries like Peru to leverage existing models by tailoring them to their specific environment. This tailored approach can be a powerful tool for developing countries to improve and build their cybersecurity on a national level.