bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD271 regulates the proliferation and motility of hypopharyngeal cancer cells
Authors: Mochizuki Mai , Tamai Keiichi , Imai Takayuki , Sugawara Sayuri , Ogama Naoko , Nakamura Mao , Matsuura Kazuto , Yamaguchi Kazunori , Satoh Kennichi , Sato Ikuro , Motohashi Hozumi , Sugamura Kazuo , Tanaka Nobuyuki 
Published: July 2016

AbstractCD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G0 and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G0 arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC.
The origin of chromosomal inversions as a source of segmental duplications in the Sophophora subgenus of Drosophila
Authors: Puerma Eva , Orengo Dorcas J. , Aguadé Montserrat 
Published: July 2016

AbstractChromosomal inversions can contribute to the adaptation of organisms to their environment by capturing particular advantageous allelic combinations of a set of genes included in the inverted fragment and also by advantageous functional changes due to the inversion process itself that might affect not only the expression of flanking genes but also their dose and structure. Of the two mechanisms originating inversions —ectopic recombination and staggered double-strand breaks and subsequent repair— only the latter confers the inversion the potential to have dosage effects and/or to generate advantageous chimeric genes. In Drosophila subobscura, there is ample evidence for the adaptive character of its chromosomal polymorphism, with an important contribution of some warm-climate arrangements such as E1+2+9+12. Here, we have characterized the breakpoints of inversion E12 and established that it originated through the staggered-break mechanism like four of the five inversions of D. subobscura previously studied. This mechanism that also predominates in the D. melanogaster lineage might be prevalent in the Sophophora subgenus and contribute to the adaptive character of the polymorphic and fixed inversions of its species. Finally, we have shown that the D. subobscura inversion breakpoint regions have generally been disrupted by additional structural changes occurred at different time scales.
An integrated process for the extraction of fuel and chemicals from marine macroalgal biomass
Authors: Trivedi Nitin , Baghel Ravi S. , Bothwell John , Gupta Vishal , Reddy C. R. K. , Lali Arvind M. , Jha Bhavanath 
Published: July 2016

AbstractWe describe an integrated process that can be applied to biomass of the green seaweed, Ulva fasciata, to allow the sequential recovery of four economically important fractions; mineral rich liquid extract (MRLE), lipid, ulvan and cellulose. The main benefits of our process are: a) its simplicity and b) the consistent yields obtained from the residual biomass after each successive extraction step. For example, dry Ulva biomass yields ~26% of its starting mass as MRLE, ~3% as lipid, ~25% as ulvan and ~11% as cellulose, with the enzymatic hydrolysis and fermentation of the final cellulose fraction under optimized conditions producing ethanol at a competitive 0.45 g/g reducing sugar. These yields are comparable to those obtained by direct processing of the individual components from primary biomass. We propose that this integration of ethanol production and chemical feedstock recovery from macroalgal biomass could substantially enhance the sustainability of marine biomass use.
Effect of leptin combined with CoCl2 on healing in Sprague Dawley Rat fracture model
Authors: Liu Pengcheng , Liu Junfeng , Xia Kuo , Chen Liyang , Wu Xing 
Published: July 2016

AbstractTo evaluate the effect of leptin combined with CoCl2 on rat femur fracture healing. 48 male Sprague Dawley rats were randomly divided into two main groups. Then standardized femur fractures were created to all rats. Control group rats were treated with 0.5 mL physiological saline, and experimental group rats were treated with 5 μg/Kg.d leptin and 15 mg/Kg.d CoCl2 along with 0.5 mL physiological saline for 42 days intraperitoneally. Each main group was divided into three subgroups for each evaluation at second, fourth and sixth weeks, each subgroup included eight rats. The radiological evaluation showed that the fracture healing progress of experimental group was superior to control group from second week. At fourth week, experimental group had better fracture healing progress than control group significantly. Results of biomechanics show the ultimate load (N) and deflection ultimate load (mm) of experimental group was significantly increased than that in control group from fourth week. The present result demonstrated that leptin combined with CoCl2 significantly increased the mRNA expression levels of HIF1A, Vegfa, Runx2, Bmp2, Bglap and Alpl. It suggested that leptin combined with CoCl2 have a positive effect on rat femur fracture healing by activating the HIF1A pathway.
NLRP3 protects alveolar barrier integrity by an inflammasome-independent increase of epithelial cell adherence
Authors: Kostadinova Elena , Chaput Catherine , Gutbier Birgitt , Lippmann Juliane , Sander Leif E. , Mitchell Timothy J. , Suttorp Norbert , Witzenrath Martin , Opitz Bastian 
Published: August 2016

AbstractBacterial pneumonia is a major cause of acute lung injury and acute respiratory distress syndrome, characterized by alveolar barrier disruption. NLRP3 is best known for its ability to form inflammasomes and to regulate IL-1β and IL-18 production in myeloid cells. Here we show that NLRP3 protects the integrity of the alveolar barrier in a mouse model of Streptococcus pneumoniae-induced pneumonia and ex vivo upon treatment of isolated perfused and ventilated lungs with the purified bacterial toxin, pneumolysin. We reveal that the preserving effect of NLRP3 on the lung barrier is independent of inflammasomes, IL-1β and IL-18. NLRP3 improves the integrity of alveolar epithelial cell monolayers by enhancing cellular adherence. Collectively, our study uncovers a novel function of NLRP3 by demonstrating that it protects epithelial barrier function independently of inflammasomes.
Neural mechanisms of transient neocortical beta rhythms: Converging evidence from humans, computational modeling, monkeys, and mice
Authors: Sherman Maxwell A. , Lee Shane , Law Robert , Haegens Saskia , Thorn Catherine A. , Hämäläinen Matti S. , Moore Christopher I. , Jones Stephanie R. 
Published: July 2016

Significance
Neocortical beta is one of the most prominent signatures of neural activity measured noninvasively in humans. Beta expression is a strong predictor of healthy and pathological perceptual and motor performance. However, there is considerable debate as to whether beta itself is causally important in information and disease processes. Key to resolving this debate is understanding the neural mechanisms inducing beta. Here, building on prior work, we combined human magnetoencephalography, computational modeling, and laminar recordings in mice and monkeys to establish and test a new theory explaining the emergence of spontaneous transient neocortical beta events in somatosensory and frontal cortex. Our results enable a principled understanding of neocortical beta and can help guide studies seeking to understand its relation to function.
An Advanced Protocol‐Driven Transition from Parenteral Prostanoids to Inhaled Trepostinil in Pulmonary Arterial Hypertension
Authors: Oudiz Ronald , Agarwal Manyoo , Rischard Franz , De Marco Teresa 
Published: August 2016

Patients with pulmonary arterial hypertension (PAH) often require parenteral prostanoids to improve symptoms and signs of PAH. Complications of parenteral prostanoids—such as catheter‐related infections and intolerable adverse effects—may develop, prompting transition to inhaled prostanoids. We report a prospective, protocol‐driven transition from parenteral prostanoids to inhaled prostanoids with monitoring of exercise gas exchange and acute hemodynamics. Three PAH centers recruited patients transitioning from parenteral prostanoids to inhaled trepostinil. Rigid inclusion criteria were used, including parenteral prostanoid dose < 30 ng/kg/min, New York Heart Association functional class (FC) < 3, and pulmonary vascular resistance (PVR) < 6 Wood units. Of the 9 patients meeting initial inclusion criteria, 3 were excluded. In the remaining patients, the parenteral prostanoid was reduced and the inhaled prostanoid was increased over 24–36 hours with continuous hemodynamic monitoring. Exercise capacity and FC were measured at baseline and weeks 1, 4, and 12. All patients were successfully weaned from parenteral prostanoids. An acute PVR decrease was seen with most inhaled prostanoid doses, but PVR varied throughout the transition. Patients tolerated inhaled prostanoids for 9–12 breaths 4 times a day with no treatment‐limiting adverse events. At week 12, FC was unchanged, and all patients continued to receive inhaled prostanoids without serious adverse events or additional PAH therapy. In 5 of 6 patients, 6‐minute walk distance and peak V̇O2 were within 10% of baseline. Using a strict transition protocol and rigid patient selection criteria, the parenteral prostanoid to inhaled prostanoid transition appeared safe and well tolerated and did not result in clinical deterioration over 12 weeks. Hemodynamic variability noted acutely during transition in our study did not adversely affect successful transition. (Trial registration: ClinicalTrials.gov identifier: NCT01268553)
miR-423-5p contributes to a malignant phenotype and temozolomide chemoresistance in glioblastomas
Authors: Li Shouwei , Zeng Ailiang , Hu Qi , Yan Wei , Liu Yanwei , You Yongping 
Published: July 2016

Abstract

Background
Gliomas are based on a genetic abnormality and present with a dismal prognosis. MicroRNAs (miRNAs) are considered to be important mediators of gene expression in glioma tissues.

Methods
Real-time PCR was used to analyze the expression of microRNA-423-5p (miR-423-5p) in human glioma samples and normal brain tissue. Apoptosis, cell cycle, proliferation, immunostaining, transwell, in vitro 2D and 3D migration, and chemosensitivity assays were performed to assess the phenotypic changes in glioma cells overexpressing miRNA-423-5p. Western blotting was used to determine the expression of inhibitor of growth 4 (ING-4)in glioma tissues, and a luciferase reporter assay was conducted to confirm whether ING-4 is a direct target of miR-423-5p. Western blotting was used to identify the potential signaling pathways that are affected in glioma cell growth by miR-423-5p. Xenograft tumors were examined in vivo for the carcinogenic effects of miR-423-5p in glioma tissues.

Results
We first reported that miR-423-5p expression was increased in gliomas and was a potential tumor promoter via targeting ING-4. The overexpression of miR-423-5p resulted in upregulation of important signaling molecules such as p-AKT and p-ERK1/2. In clinical samples, miR-423-5p was dysregulated, and a corresponding alteration in ING-4 expression was observed (P = .0207). Furthermore, the overexpression of miR-423-5p strengthened glioma cell proliferation, angiogenesis, and invasion. Finally, miR-423-5p overexpression also strengthened GBM neurosphere formation and rendered glioma cells resistant to temozolomide (TMZ).

Conclusion
This study establishes that miR-423-5p functions as an oncogene in glioma tissues by suppressing ING-4 and suggests that it has therapeutic potential for glioma.
Knowledge network dynamics in clusters: past performance and absorptive capacity
Authors: Belso-Martínez José-Antonio , Expósito-Langa Manuel , Tomás-Miquel José-Vicente 
Published: July 2016

Purpose– The purpose of this paper is to explore the degree to which absorptive capacity and previous innovative performance affect network dynamics, specifically in the creation or destruction of inter-business relationships.Design/methodology/approach– The empirical study has drawn on the data collected in an industrial cluster located in the Valencian Community in Spain. This population of firms allowed us to test various roles played by network endogenous forces, absorptive capacity and former performance in the creation and dissolution of inter-organisational linkages. The authors followed an evolutionary approach and applied network analysis techniques.Findings– Empirical evidences suggest that absorptive capacity and previous innovative performance are predictors of inter-business relationships. Absorptive capacity affects the emergence of linkages in the technological network, due to the tacit nature of technological knowledge. On the other hand, previous innovative performance is an indicator to identify leaders companies. These companies tend to increase the reception of request for advice from local firms. Moreover, prestigious companies tend to be more selective in choosing collaborators.Practical implications– This study helps researchers and managers better understand network dynamics. The generation of inter-organisational relationships in clusters can be determined by cognitive proximity and prestigious status in the network. These evidences are relevant in a mature cluster where knowledge is asymmetrically distributed.Originality/value– Over the last few decades, studies on industrial clusters have evolved from the “canonical” standpoint. Nowadays, researchers usually take a more sophisticated and richer view of this reality, mainly as a consequence of the inclusion the proximity concept, intra-cluster heterogeneity and advanced analysis of overlapping between networks and territory. Thus, the authors try to add some empirical evidence along these lines.
Numerical simulation and experiment validation of level-pour direct-chill casting of A390 alloy hollow billets under different feeding schemes
Authors: Zuo Kesheng , Zhang Haitao , Qin Ke , Cui Jianzhong 
Published: August 2016

Purpose
– The purpose of this paper is to study the effect of feeding scheme on melt flow and temperature field during the steady-state of level-pour direct-chill (DC) casting of A390 alloy hollow billet and optimize the design of feeding scheme.

Design/methodology/approach
– Melt flow and temperature field are investigated by numerical simulation, which is based on a three-dimensional mathematical model and well verified by experiments.

Findings
– The numerical results reveal that both melt flow and temperature field are obviously affected by the feeding scheme. The homogeneity of melt flow and temperature field in hollow billet with the feeding scheme of modified four inlets are better than the other feeding schemes. Experimental results show that crack can be eliminated by increasing the number of feeding inlets. The primary Si size appears unaffected while the distribution of primary Si particles is highly affected by the change of feeding scheme. Only with the feeding scheme of modified four inlets can fine and uniformly distributed primary Si particles be achieved.

Practical implications
– The paper includes implications for the design of feeding scheme in level-pour DC casting of hollow billet for practical use.

Originality/value
– This paper develops different feeding schemes for level-pour DC casting of hollow billet and optimizes the design of feeding scheme.
Numerical analysis of combined buoyancy-induced and pressure-driven smoke flow in complex vertical shafts during building fires
Authors: Chen Yanqiu , Zhou Xiaodong , Zhang Taolin , Fu Zhijian , Hu Yuqi , Yang Lizhong 
Published: August 2016

Purpose
– The purpose of this paper is to study the behavior of smoke flow in building fires and optimize the design of smoke control systems.

Design/methodology/approach
– A total of 435 3-D fire simulations were conducted through NIST fire dynamics simulator to analyze thermal behavior of combined buoyancy-induced and pressure-driven smoke flow in complex vertical shafts, under consideration of influence of heat release rate (HRR) and locations of heat sources. This influence was evaluated through neutral pressure plane (NPP), which is a critical plane depicting the flow velocity distributions. Hot smoke flows out of shafts beyond the NPP and cold air flows into shafts below the NPP.

Findings
– Numerical simulation results show that HRR of heat source has little influence on NPP, while location of heat source can make a significant difference to NPP, particularly in cases of multi-heat source. Identifying the location of NPP helps to develop a more effective way to control the smoke with less energy consumption. Through putting an emphasis on smoke exhausting beyond the NPP and air supplying below the NPP, the smoke control systems can make the best use of energy.

Research limitations/implications
– Because of the chosen research approach, the research results may need to be tested by further experiments.

Practical implications
– The paper includes implications for the optimization of smoke control systems design in buildings.

Originality/value
– This paper fulfills an identified need to research the behavior of hot smoke in building fires and optimize the design of smoke control systems.
Simultaneous geometry and cross-section optimization of aluminum trusses
Authors: Kripka Moacir , Pravia Zacarias Chamberlain , Medeiros Guilherme Fleith , Dias Maiga Marques 
Published: August 2016

Purpose
– Trusses constitute a fertile field to demonstrate the application of optimization techniques because of the possibility of several different configurations. Using such techniques allows the search for designs that minimize the use of material to safely comply with the imposed loads. Truss optimization can be classified into three categories: cross-section, shape, and topology. The purpose of this paper is to present a numerical and experimental study developed to minimize the weight of aluminum trusses, taking both the cross-sectional dimensions of the elements and the nodal coordinates as design variables.

Design/methodology/approach
– Initially, several numerical computer simulations were performed with an optimization program developed by combining the displacement method and a simulated annealing optimization method. Subsequently, two aluminum trusses were selected and built in order to validate the numerical results obtained.

Findings
– Experimental tests verified the excellent performance of the optimized model.

Originality/value
– In addition, it was concluded that significant savings could be obtained from the application of the proposed formulation.
Managing celebrity via impression management on social network sites
Authors: Agyemang Kwame J.A. , S. Williams Antonio 
Published: July 2016

PurposeCentral to the celebrity creation process is mass media communication and impression management (IM) behaviors of social actors. The emergence of social network sites (SNSs) such as Twitter offers a platform for social actors to engage both of these means in efforts to manage their celebrity. The purpose of this paper is to contribute to the understanding of how celebrity athletes manage their celebrity status by investigating IM tactics employed by National Basketball Association (NBA) celebrities on Twitter.Design/methodology/approachA content analytic design was employed to examine the Twitter posts of the top ten most popular and influential NBA celebrity athletes (past and present) at the time of tweet acquisition.FindingsThe findings revealed the celebrity athletes used a variety of IM tactics to manage their celebrity. Defensive IM tactics (i.e. reactive measures taken) were used sparingly when compared to offensive IM tactics (i.e. proactive measures taken). Also, consistent with extant IM literature, the celebrity athletes utilized IM tactics in isolation as well as in combination.Practical implicationsThe extant literature suggests that celebrities cultivate their relationships with the various media outlets with the potential to create (or even damage) one’s celebrity. This study offers celebrity athletes and their managers with useful insight on celebrity management.Originality/valueThis study is the first to examine IM in a sport business context, particularly the use of IM of athletes on SNSs.
Draft Genome Sequences of the Antimicrobial Producers
Pseudomonas
sp. TAA207 and
Pseudomonas
sp. TAD18 Isolated from Antarctic Sediments
Authors: Presta Luana , Inzucchi Ilaria , Bosi Emanuele , Fondi Marco , Perrin Elena , Maida Isabel , Miceli Elisangela , Tutino Maria Luisa , Lo Giudice Angelina , de Pascale Donatella , Fani Renato 
Published: July 2016

ABSTRACT

We report here the draft genome sequence of the
Pseudomonas
sp. TAA207 and
Pseudomonas
sp. TAD18 strains, isolated from Antarctic sediments during a summer campaign near coastal areas of Terra Nova Bay (Antarctica). Genome sequence knowledge allowed the identification of genes associated with the production of bioactive compounds and antibiotic resistance. Furthermore, it will be instrumental for comparative genomics and the fulfillment of both basic and application-oriented investigations.
Whole-Genome Draft Sequences of Six Commensal Fecal and Six Mastitis-Associated
Escherichia coli
Strains of Bovine Origin
Authors: Leimbach Andreas , Poehlein Anja , Witten Anika , Wellnitz Olga , Shpigel Nahum , Petzl Wolfram , Zerbe Holm , Daniel Rolf , Dobrindt Ulrich 
Published: July 2016

ABSTRACT

The bovine gastrointestinal tract is a natural reservoir for commensal and pathogenic
Escherichia coli
strains with the ability to cause mastitis. Here, we report the whole-genome sequences of six
E. coli
isolates from acute mastitis cases and six
E. coli
isolates from the feces of udder-healthy cows.