bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hedgehog pathway activation in T-cell acute lymphoblastic leukemia predicts response to SMO and GLI1 inhibitors
Authors: Dagklis Antonis , Demeyer Sofie , De Bie Jolien , Radaelli Enrico , Pauwels Daphnie , Degryse Sandrine , Gielen Olga , Vicente Carmen , Vandepoel Roel , Geerdens Ellen , Uyttebroeck Anne , Boeckx Nancy , de Bock Charles E. , Cools Jan 
Published: September 2016

Key Points
A subset of T-ALL cases show high expression of hedgehog pathway genes including the SHH ligand and the GLI1 transcription factor. T-ALL samples with high GLI1 expression levels respond to hedgehog inhibitor treatment in vitro and in vivo.
Características clínicas, prevalência e diagnóstico de vulvovaginites em ambulatório do interior do Rio Grande do Sul<br>Clinical characteristics and prevalence of vulvovaginitis in a clinic in the interior of the Rio Grande do Sul<br>doi:10.12662/2317-3076jhbs.v4i3.657.p160-165.2016
Authors: Tabile Patrícia Micheli , Lucena Hérika , Chaves Jéssica , Fischborn Juliana , Jucá Renata Becker 
Published: September 2016

Introdução: As vulvovaginites são uma das principais queixas no atendimento rotineiro de ginecologia. O diagnóstico correto e o tratamento precoce dessas afecções são importantes além de prevenir possíveis repercussões no trato genital superior. Objetivo: Conhecer as características clínicas e a prevalência das vulvovaginites. Metodologia: Estudo transversal de natureza exploratória e descritiva, de abordagem quantitativa sobre mulheres que foram atendidas na Unidade de Atendimento de um Ambulatório de Ginecologia do Sistema Integrado de Saúde (SIS) na Universidade de Santa Cruz do Sul, no período de setembro de 2014 a maio de 2015. As variáveis analisadas foram: idade, queixas clínicas, exame ginecológico e achados no exame a fresco. Os dados foram analisados no SPSS 22.0. Resultados: Do total de 200 pacientes atendidas no ambulatório, 66 (33%) foram selecionadas para realização do exame a fresco. A leucorreia fisiológica foi diagnosticada em 35 pacientes (53,0%), a vaginose bacteriana em 24 (36,5%), a candidíase em 6 (9,0%) e a tricomoníase em 1 (1,5%). Na vaginose bacteriana leucorreia com odor fétido, na candidíase leucoréia grumosa branca, prurido, ardência, vulva eritematosa e dispaurenia foram significativos para o diagnóstico (p < 0,05). Conclusões: Observou-se que a faixa etária das mulheres acometidas por vulvovaginites foi compatível com a literatura, predominando em idade reprodutiva. Constatou-se que a vaginose bacteriana foi a mais diagnosticada e que a leucorreia com odor fétido foi o sintoma estatisticamente significativo para o diagnóstico. Da mesma forma, a candidíase, com leucorreia grumosa branca, prurido, ardência, vulva eritematosa e dispareunia.
Electronic medical records in humanitarian emergencies – the development of an Ebola clinical information and patient management system
Authors: Jobanputra Kiran , Greig Jane , Shankar Ganesh , Perakslis Eric , Kremer Ronald , Achar Jay , Gayton Ivan 
Published: September 2016

By November 2015, the West Africa Ebola epidemic had caused 28598 infections and 11299 deaths in the three countries most affected. The outbreak required rapid innovation and adaptation. Médecins sans Frontières (MSF) scaled up its usual 20-30 bed Ebola management centres (EMCs) to 100-300 beds with over 300 workers in some settings. This brought challenges in patient and clinical data management resulting from the difficulties of working safely with high numbers of Ebola patients. We describe a project MSF established with software developers and the Google Social Impact Team to develop context-adapted tools to address the challenges of recording Ebola clinical information. We share the outcomes and key lessons learned in innovating rapidly under pressure in difficult environmental conditions. Information on adoption, maintenance, and data quality was gathered through review of project documentation, discussions with field staff and key project stakeholders, and analysis of tablet data. In March 2015, a full prototype was deployed in Magburaka EMC, Sierra Leone. Inpatient data were captured on 204 clinical interactions with 34 patients from 5 March until 10 April 2015. Data continued to also be recorded on paper charts, creating theoretically identical record “pairs” on paper and tablet. 85 record pairs for 32 patients with 26 data items (temperature and symptoms) per pair were analysed. The average agreement between sources was 85%, ranging from 69% to 95% for individual variables. The time taken to deliver the product was more than that anticipated by MSF (7 months versus 6 weeks). Deployment of the tablet coincided with a dramatic drop in patient numbers and thus had little impact on patient care. We have identified lessons specific to humanitarian-technology collaborative projects and propose a framework for emergency humanitarian innovation. Time and effort is required to bridge differences in organisational culture between the technology and humanitarian worlds. This investment is essential for establishing a shared vision on deliverables, urgency, and ownership of product.
Treatment of large, complex, non-healing wounds with cryopreserved amniotic suspension allograft: a case series
Authors: Marcus B. 
Published: September 2016

Objective: Cryopreserved amniotic suspension allograft (CASA) is a regenerative tissue suspension containing amniotic membrane and amniotic fluid components used for soft-tissue repair, replacement, and reconstruction. The aim of this case series is to examine its effects in different wound types. Method: In a retrospective series CASA was applied to chronic non-healing wounds. All patients were treated at a single center between June 2013 and December 2014. The criterion for application of CASA was lack of progress toward wound healing despite standard treatments and adjuvant therapies. Results: We assessed five patients aged between 39 to 86 years old. Three patients had surgical/radiation wounds, one had a sacral pressure ulcer, and one had a diabetic foot ulcer (DFU). All patients experienced progressive improvement in wound area and volume during treatment with CASA. One patient (postmastectomy tissue necrosis) required surgical closure in conjunction with removal of retained sutures. CASA applied topically and subcutaneously was particularly well suited to application in these wounds with large sinus tracts or undermining. All five patients experienced complete wound closure following one to two applications of CASA over 5 to 22 weeks. Conclusion: This series highlights the effective use of CASA in recalcitrant large soft-tissue wounds, DFUs, and deep wounds with tendon exposure. Declaration of interest: This study was supported by a grant from Derma Sciences (Princeton, NJ, US).
Viable intact cryopreserved human placental membrane for a non-surgical approach to closure in complex wounds
Authors: Suzuki K. , Michael G. , Tamire Y. 
Published: September 2016

Objective: Advances in wound treatment have yielded successful approaches to complex wound closure through plastic surgery or other staged surgical methods. However, not all patients or wounds are candidates for such increasingly rigorous levels of interventions. The exploration of non-surgical treatment options that promote anatomical and functional tissue replacement on an outpatient basis without donor site morbidity is necessary to maximise opportunities for patient mobility and rehabilitation. The native components found in human placental membranes may offer alternative properties for the clinical management of these complex wounds. Method: A 10-month, single-centre effectiveness review was conducted on the clinical outcomes in patients (n=12) treated with Grafix, a viable intact cryopreserved human placental membrane (vCPM), for complex wounds characterised by exposed bone, tendon, muscle, or hardware. Wounds were treated with routine standard of care in addition to weekly grafting with a vCPM. Results: The average baseline wound area and duration at presentation were 17.5 cm2 and 11.2 weeks. All twelve cases resulted in granulation over the exposed structures followed by complete wound re-epithelialisation without the concomitant use of negative pressure wound therapy, hyperbaric oxygen therapy, or surgical intervention. The mean 4-week percentage area reduction was 62.5 %. The mean time to closure was 10 weeks with a mean graft use of 8.1 grafts. Of the wounds seven (58%) presented with exposed tendon associated with an actively moving joint, the functional integrity of which was maintained with a return to the previous range of motion upon wound closure. Conclusion: These outcomes illustrate that vCPM is a clinically effective option in the non-surgical management of complex wounds in the outpatient setting, contributing to timely definitive closure without the risk of associated donor site morbidity. Declaration of interest: Kazu Suzuki, DPM, CWS is a member of the Speakers' Bureau at Osiris Therapeutics Inc., Georgina Michael and Yeabsera Tamire belong to the Department of Medical Affairs at Osiris Therapeutics Inc.
The long-term outcome of childhood supracondylar humeral fractures
Authors: Sinikumpu J-J. , Victorzon S. , Pokka T. , Lindholm E-L. , Peljo T. , Serlo W. 
Published: September 2016

AimsWe present the clinical and radiographic outcome of 81 children with Gartland type I to III supracondylar humeral fractures at a minimum follow-up of ten years (mean 12.1 years; 10.3 to 16.1) following injury.Patients and MethodsThe clinical and functional outcomes are compared with normal age- and gender-matched individuals. The population-based study setting was first identified from the institutional registries; the rate of participation was 76%. Controls were randomly selected from Finnish National Population Registry.ResultsAccording to Flynn's criteria, most fractures (75.3%) resulted in a satisfactory (“good or excellent”) outcome. Satisfactory recovery was achieved in 75.0% of type I fractures treated by closed splinting (p = 0.013). Type II fractures were associated with both satisfactory (57.7%) and unsatisfactory (42.3%) results, regardless of the type of treatment, although the numbers were small in the sub groups. Most type III fractures were treated operatively, and most (76%) had a satisfactory outcome according to Flynn’s criteria (p = 0.015). Compared with none among the normal subjects, flexion of the elbow was reduced by > 10° at long-term follow-up in 20 cases (24.7%, p < 0.001) and 9 (11.1%) had a reduced flexion of > 15° (p = 0.004). In patients who had sustained a type III fracture, the carrying angle was decreased by 35.7% (from 9.8° to 6.3°; p = 0.048). All patients achieved an excellent Mayo Elbow Performance Score (mean 96.4 points).ConclusionThe long-term outcome of extension-type supracondylar humeral fractures is generally good, but not exclusively benign, with the potential for long-term pain and ulnar nerve sensitivity, and a decrease in grip strength and range of movement in type II and type III fractures. Bony remodelling cannot be relied upon to correct any residual deformity. In particular, type II fractures have impaired long-term recovery and justify individual consideration in their treatment. Cite this article: Bone Joint J 2016;98-B:1410–17.
Comparison of femoral component migration between Refobacin bone cement R and Palacos R + G in cemented total hip arthroplasty
Authors: van der Voort P. , Valstar E. R. , Kaptein B. L. , Fiocco M. , van der Heide H. J. L. , Nelissen R. G. H. H. 
Published: September 2016

AimsThe widely used and well-proven Palacos R (a.k.a. Refobacin Palacos R) bone cement is no longer commercially available and was superseded by Refobacin bone cement R and Palacos R + G in 2005. However, the performance of these newly introduced bone cements have not been tested in a phased evidence-based manner, including roentgen stereophotogrammetric analysis (RSA).Patients and MethodsIn this blinded, randomised, clinical RSA study, the migration of the Stanmore femoral component was compared between Refobacin bone cement R and Palacos R + G in 62 consecutive total hip arthroplasties. The primary outcome measure was femoral component migration measured using RSA and secondary outcomes were Harris hip score (HHS), Hip disability and Osteoarthritis Outcome Score (HOOS), EuroQol 5D (EQ-5D) and Short Form 36 (SF-36).ResultsFemoral component migration was comparable between Refobacin bone cement R and Palacos R + G during the two-year follow-up period with an estimated mean difference of 0.06 mm of subsidence (p = 0.56) and 0.08° of retroversion (p = 0.82). Five hips (three Refobacin bone cement R and two Palacos R + G) showed non-stabilising, continuous migration; the femoral cement mantle in these hips, was mean 0.7 mm thicker (p = 0.02) and there were more radiolucencies at the bone-cement interface (p = 0.004) in comparison to hips showing stabilising migration. Post-operative HHS was comparable throughout the follow-up period (p = 0.62). HOOS, EQ5D, and SF-36 scores were also comparable (p-values > 0.05) at the two-year follow-up point.ConclusionRefobacin bone cement R and Palacos R + G show comparable component migration and clinical outcome during the first two post-operative years. Hips showing continuous migration are at risk for early failure. However, this seems to be unrelated to cement type, but rather to cementing technique. Cite this article: Bone Joint J 2016;98-B:1333–41.
Abnormalities of gait caused by ankle arthritis are improved by ankle arthrodesis
Authors: Brodsky J. W. , Kane J. M. , Coleman S. , Bariteau J. , Tenenbaum S. 
Published: September 2016

Aims The surgical management of ankle arthritis with tibiotalar arthrodesis is known to alter gait, as compared with normal ankles. The purpose of this study was to assess post-operative gait function with gait before arthrodesis. Patients and Methods We prospectively studied 20 patients who underwent three-dimensional gait analysis before and after tibiotalar arthrodesis. Cadence, step length, walking velocity and total support time were assessed. Kinetic parameters, including the moment and power of the ankle in the sagittal plane and hip power were also recorded. Results Significant improvement was recorded across numerous parameters compared with pre-operative measurements. Temporal-spatial data demonstrated a significant increase in step length (p = 0.003) and velocity (p = < 0.001). Total support time decreased for the unaffected limb (p = 0.01). Kinematic results demonstrated that in the affected limb, total sagittal range of movement did not change significantly (p = 0.1259). However, the arc of movement had a near congruent shift with mean maximal dorsiflexion increasing from 5° (-17° to 16°) to 12° (5° to 18°) (p < 0.001) and mean maximal plantarflexion decreasing from 6.8° (6° to 21°) to 0.9° (-9° to 8°) (p = 0.003). Mean hip joint range of movement increased by 6° (-7° to 24°; p = 0.003). Kinetic results demonstrated no statistically significant change in ankle power (p = 0.1292). However, there was an increase in ankle moment (p = 0.04) and hip power (p = 0.01) in the surgically treated extremity. Sagittal plane range of movement was not reduced after tibiotalar fusion. Conclusion Although following tibiotalar arthrodesis the gait demonstrated never matched the gait shown in unaffected ankles, compared with the pre-operative analysis there was improvement in numerous temporal-spatial, kinematic, and kinetic measures. Cite this article: Bone Joint J 2016;98-B:1369–75.
Lyapunov Matrices Approach to the Parametric Optimization of a System with Two Delays
Authors: Duda Jozef 
Published: September 2016

AbstractIn the paper a Lyapunov matrices approach to the parametric optimization problem of time-delay systems with two commensurate delays and a P-controller is presented. The value of integral quadratic performance index of quality is equal to the value of the Lyapunov functional for the initial function of time-delay system. The Lyapunov functional is determined by means of the Lyapunov matrix.
Characterization of Intor:Swiss Albino Mice Adopted in the Institute of Virology, Vaccines and Sera – Torlak, Belgrade in the Early Twentieth Century
Authors: Živković Irena , Rajnpreht Irena , Minić Rajna , Mitić Katarina , Aleksić Iva , Kadrić Jasminka , Petrušić Vladimir 
Published: September 2016

AbstractThe Institute of Virology, Vaccines and Sera Torlak was established in 1927, while the first vaccine was produced in the Institute in 1930. Vaccines production implies using experimental animals, including mice, in in-process controls. The laboratory mice which have been in use in Torlak Institute from the very beginning belong to Swiss albino outbred stock. This stock, which has been in use for more than 80 years contains a large number of mice maintained at all times, was recently named Intor:Swiss. Biological characteristics of Intor:Swiss stock, are presented in this paper for the first time. Taking into account the presented characteristics, the Institute Torlak’s Swiss mice are suitable for use in pharmaceutical studies, vaccine development research and basic research, as well as in toxicological studies. The publication of data on the Intor:Swiss mice represents a contribution to the international scientific community, since it offers the possibility for obtaining an additional outbred mouse stock for research.
The Influence of Different Chemical Forms of Selenium Added to the Diet Including Carnosic Acid, Fish Oil and Rapeseed Oil on the Formation of Volatile Fatty Acids and Methane in the Rumen, and Fatty Acid Profiles in the Rumen Content and Muscles of Lambs
Authors: Miltko Renata , Rozbicka-Wieczorek J. Agnieszka , Więsyk Edyta , Czauderna Marian 
Published: September 2016

AbstractLambs were divided into 3 groups of 6 animals each. For 35 days lambs were fed a diet including 2% rapeseed oil, 1% fish oil and 0.1% carnosic acid (the control group) or two experimental diets supplemented with 0.35 mg ∙ kg−1Se as selenized-yeast (SeY) (the SeY diet) or selenate (the selenate diet). Muscles (Musculus longissimus dorsi(MLD) andMusculus biceps femoris(MBF)), ruminal fluids and microbiota were collected from each lamb. SeY supplementation most effectively stimulated the accumulation of straight-chain volatile fatty acids (VFAs),iso-branched-chain VFAs, CO2and CH4in the ruminal fluid. The contents of CO2, CH4and VFAs including straight-chain VFAs with the exception ofiso-branched-chain VFAs were most effectively reduced by the selenite diet. The control diet most efficiently increased the concentration sums ofodd-saturated fatty acids (odd-SFAs) andiso-SFAs in microbiota. The SeY diet most efficiently reduced acetic acid to propionic acid ratio in the ruminal fluid. The selenate diet improved animal performance by reducing ruminal concentrations of CH4and CO2. The SeY diet and especially the selenate diet reduced the biohydrogenation to C18:0 when compared with the control diet. The selenate diet more efficiently reduced the concentration sums of all SFAs (ΣSFAs) and all fatty acids (ΣFAs) inMLDandMBFthan the SeY diet, which most effectively increased the concentrations of ΣSFAs and ΣFAs inMLDandMBF. The selenate diet most effectively increased the body mass gain of lambs.
Sign-changing two-peak solutions for an elliptic free boundary problem related to confined plasmas
Authors: Pisante Giovanni , Ricciardi Tonia 
Published: September 2016

Abstract
By a perturbative argument, we construct solutions for a plasma-type problem with two
opposite-signed sharp peaks at levels 1 and

-
γ

{-\gamma}

, respectively, where

0
<
γ
<
1

{0<\gamma<1}

. We establish some physically relevant qualitative properties for such solutions,
including the connectedness of the level sets
and the asymptotic location of the peaks as

γ
→

0
+

{\gamma\to 0^{+}}

.
Strukturwandel der Wirtschaft und Entlastung der Umwelt
Authors: Simonis Udo E. 
Published: September 2016

Abstract
Schrumpfen und Wachsen sind, wie die theoretische Reflexion zeigt, inhärente Bestandteile des Strukturwandels der Wirtschaft und sie sollten, wie die empirische Analyse erweist zu elementaren Bestandteilen einer ökologischen Strukturpolitik werden. In diesem Aufsatz wird anhand vergleichender empirischer Studien festgestellt dass der autonome wirtschaftliche Strukturwandel zwar eine notwendige, jedoch keine hinreichende Bedingung einer nachhaltigen Entwicklung (sustainable development) ist. In der Praxis ist Politik daher weiterhin gefragt – eine effektive ökologische Politik allerdings, die vor allem – aber nicht ausschließlich – von zwei Ressorts formuliert und implementiert werden muss: der Umwelt und der Wirtschaftspolitik.
Measuring Drama in Goose-like Games
Authors: Neto J.P. , Silva J.N. 
Published: September 2016

Abstract
For games of complete information with no chance component, like Chess, Go, Hex, and Konane, some parameters have been identified that help us understand what makes a game pleasant to play. One of these goes by the name of drama.
Briefly, drama is linked to the possibility of recovering from a seemingly weaker position, if the player is strong enough. This is an important requirement to prevent initial advantages to be amplified into unavoidable and thus uninteresting victories. Drama is a feature that arguably good board games should have, since it is relevant in the perception of the play experience as pleasant.
Despite its intrinsic qualitative nature, we suggest the adaptation of the concept of drama to games of pure chance and propose a set of objective criteria to measure it. Some parameters are here used to compare Goose-like games, which we compute via computer simulation for some well-know games. A statistical analysis is performed based on the play of millions of matches done by computer simulation. The article discusses correlations and patterns found among the collected data. The methodology presented herein is general and can be used to compare other types of board games.
Human papillomavirus infection mechanism and vaccine of vulva carcinoma
Authors: Tong Qing , Zheng Lingqi , Zhao Ruiying , Xing Tianling , Li Yunbo , Lin Tong , Zhang Xuan , Jin Zhe 
Published: September 2016

AbstractVulvar carcinoma is a rare tumor occurring in female patients. Though more than 40% of vulva cancers are due to the infection of human papillomavirus (HPV), understanding of HPV and vulvar carcinoma is insufficient. HPV expression is regulated by cellular and viral transcription factors that bind to specific elements within the ligase chain reaction. These proteins bind with different affinity to host cell proteins and disrupt normal epithelial differentiation and apoptosis. Immunotherapy does not target tumors, but instead targets the host immune system. Active immunotherapy is tumor-targeting or immune-targeting monoclonal antibodies and vaccines. Nonspecific active immunotherapy is mainly cytokine therapy. In the treatment and prevention of HPV, the most popular research projects were regarding peptide, recombinant protein and DNA-based vaccines, recombinant virus and other targets in HPV infection. Since the cervix and vulva are both susceptible areas, these studies may be able to help reduce prevalence of vulvar precancerous lesions and prevent all cancers caused by HPV.