bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying risk factors for recurrent multidrug resistant tuberculosis based on patient’s record data from 2016 to 2021: retrospective study
Authors: Wotale Teramaj Wongel , Lelisho Mesfin Esayas , Negasa Bikiltu Wakuma , Tareke Seid Ali , Gobena Woldemariam Erkalo , Amesa Ebsa Gelan 
Published: October 2024

AbstractGlobally, the prevalence of multidrug-resistant tuberculosis (MDR-TB) has been increasing recently. This is a major public health concern, as MDR-TB is more difficult to treat and has poorer outcomes compared to drug-sensitive tuberculosis. The main objective of the study was to identify risk factors for recurrent multidrug-resistant tuberculosis, at Alert Specialized Hospital, Addis Ababa, by using different parametric shared frailty models. From January 2016 to December 2021, a retrospective study was conducted on MDR-TB patients at Alert Specialized Hospital in Addis Ababa. The data for the study were collected from the medical records of MDR-TB patients at the hospital during this time period. Gamma and inverse-Gaussian shared frailty models were used to analyze the dataset, with the exponential, Weibull, and lognormal distributions included as baseline hazard functions. The data were analyzed using R statistical software. The median recurrence time of the patients was 12 months, and 149 (34.3%) had recurrences. The clustering effect was statistically significant for multiple drug-resistant tuberculosis patients’ recurrence. According to the Weibull-Inverse-Gaussian model, factors that reduced time to MDR-TB recurrence included lower weight (ɸ = 0.944), smoking (ɸ = 0.045), alcohol use (ɸ = 0.631), hemoptysis (ɸ = 0.041), pneumonia (ɸ = 0.564), previous anti-TB treatment (ɸ = 0.106), rural residence (ɸ = 0.163), and chronic diseases like diabetes (ɸ = 0.442) were associated with faster recurrence. While, higher education (ɸ = 3.525) and age (ɸ = 1.021) extended time to recurrence. For weight increment, smokers and alcohol users, clinical complications of hemoptysis and pneumonia, patients with pulmonary disease who had a history of previous anti-TB treatment, and being rural residents are prognostic factors. There was a significant clustering effect at the Alert Specialized Hospital in Addis Ababa, Ethiopia. The Weibull-Inverse Gaussian Shared Frailty Model was chosen as the best model for predicting the time to recurrence of MDR-TB.
Effect of three different root canal sealants on human dental pulp stem cells
Authors: Alfahlawy Ahmad , Selim Manar A. A. , Hassan Hayam Y. 
Published: October 2024

AbstractThe cytotoxic effects of three root canal sealers with different bases on human dental pulp stem cells were assessed in this study using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. The cytotoxic effects of three root canal sealers with different bases on human dental pulp stem cells (DPSCs) were assessed in this study using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. The cytotoxicity of the sealers was tested after one, 4, and 7 d. Human dental pulp stem cell proliferation was concluded using an MTT assay. Cells not treated with sealer extract were used as controls. The absorption levels were measured using an Eliza spectrophotometer. P was set at 0.05 when the percentage of cell proliferation was matched between groups and observation times using one-way analysis of variance (ANOVA).During the second passage (P2), human dental pulp stem cells displayed a single morphological and phenotypic trait, with fibroblast morphology being the most common. There were no appreciable variations between the four groups after a day. There was a notable variation in the average percentage of cell proliferation between the groups after 4 and 7 days. The control group had the highest percentage, followed by the GuttaFlow Bioseal group, the Well Root St group, and the AH-Plus group, which had the lowest percentage. For every sealing group, after one day, the highest mean percentage of cell proliferation was recorded, followed by day four, and after day seven, the lowest mean percentage. The observation periods showed minimal cytotoxic effects of GuttaFlow Bioseal, whereas AH-Plus was the most cytotoxic to human dental pulp stem cells. The highest mean percentage of cell proliferation for all sealers was recorded on day one.
Novel eco-friendly HPTLC method using dual-wavelength detection for simultaneous quantification of duloxetine and tadalafil with greenness evaluation and application in human plasma
Authors: Derayea Sayed M. , Zaafan Al Amir S. , Nagy Dalia M. , Oraby Mohamed , Amir Al , Zaafan S. 
Published: October 2024

AbstractA novel, environmentally friendly, and sensitive HPTLC method has been developed and validated for simultaneous quantification of duloxetine (DLX) and tadalafil (TDL) in their pure state, laboratory-prepared mixtures, and spiked human plasma. This method is particularly important for patients dealing with depression and sexual issues, as it allows for the measurement of these co-administered antidepressant and sexual stimulant drugs in biological fluids. The separation process employed a stationary phase of pre-coated silica gel 60 F254 and a mobile phase consisting of ethyl acetate, acetonitrile, and 33% ammonia (8:1:1, v/v). The optimized mobile phase resulted in well-defined bands for DLX and TDL, with Rf values of 0.3 and 0.8, respectively with dual-wavelength detection at 232 nm for DLX and 222 nm for TDL. Polynomial regression analysis revealed exceptional linearity for both drugs, with correlation coefficients of 0.9999 over concentration ranges of 10–900 ng/band for DLX and 10-1200 ng/band for TDL. The quantitation limits were 8.2 ng/band for DLX and 8.6 ng/band for TDL, while the detection limits (LOD) were 2.7 ng/band for DLX and 2.8 ng/band for TDL. The validation of this method followed the guidelines set by the International Council for Harmonization (ICH). Additionally, the suggested method’s greenness was assessed by means of four up-to-date ecological tools, namely the Eco-Scale, the National Environmental Method Index (NEMI), the Green Analytical Procedure Index (GAPI), and the Analytical Greenness metric approach (AGREE). The proposed method was also assessed using the Blue Applicability Grade Index (BAGI), a recently developed metric for assessing the practicality (blueness) of procedures.
Differential regulation of expression of the protein kinases DYRK1A and DYRK1B in cancer cells
Authors: Vorwerk Vincent Andreas , Wilms Gerrit , Babendreyer Aaron , Becker Walter 
Published: October 2024

AbstractThe protein kinases DYRK1A and DYRK1B are pivotal regulators of cell cycle progression by promoting cell cycle exit into quiescence. DYRK1B appears to play a more important role in cancer cell quiescence than DYRK1A, as evidenced by its overexpression or copy number variations in human tumour samples. Nonetheless, the stimuli driving DYRK1B upregulation and the potential divergence in expression patterns between DYRK1A and DYRK1B remain largely elusive. In the present study, we scrutinized the regulatory pathways modulating DYRK1B expression relative to DYRK1A in PANC-1 and A549 cancer cell lines across varying conditions. Serum deprivation, pharmacological mTOR inhibition and increased cell density resulted in the differential upregulation of DYRK1B compared to DYRK1A. We then aimed to assess the role of protein kinases MST1 and MST2, which are key transmitters of cell density dependent effects. Unexpectedly, exposure to the MST1/2 inhibitor XMU-MP-1 resulted in increased DYRK1B levels in A549 cells. Further investigation into the off-target effects of XMU-MP-1 unveiled the inhibition of Aurora kinases (AURKA and AURKB) as a potential causative factor. Consistently, AURK inhibitors VX-680 (tozasertib), MLN8237 (alisertib), AZD1152-HQPA (barasertib) resulted in the upregulation of DYRK1B expression in A549 cells. In summary, our findings indicate that the expression of DYRK1A and DYRK1B is differentially regulated in cancer cells and reveal that the kinase inhibitor XMU-MP-1 increases DYRK1B expression likely through off target inhibition of Aurora kinases.
Sub-THz and THz Cherenkov radiation source with two-dimensional periodic surface lattice and multistage depressed collector
Authors: MacLachlan Amy J. , Zhang Liang , Konoplev Ivan V. , Phelps Alan D. R. , Robertson Craig W. , MacInnes Philip , Whyte Colin G. , Ronald Kevin , Cross Adrian W. , Henderson Mark A. 
Published: October 2024

AbstractWe present the theory, concept and design of an efficient, megawatt coherent Cherenkov radiation source based on a two-dimensional periodic surface lattice (2D-PSL) cavity combined with a novel energy recovery system for the generation of highly efficient (> 50%) single-frequency radiation. We demonstrate the scalability of the transverse dimension of the 2D-PSL cavity of the Cherenkov source and thus the potential for efficient, continuous-wave, high-power (> 1 MW) operation; fundamental to the eventual realization of clean, fusion energy. These new sources, with the capacity to operate in the 0.1-10THz range, hold strong promise to address the long-standing “Terahertz gap”. By combining a Cherenkov oscillator driven by a non-gyrating beam with an innovative four-stage depressed collector energy recovery system, the overall device efficiency can be increased to be competitive with gyrotrons in the requirements for heating and current drive in fusion plasma. In these Cherenkov devices, the frequency independence of the magnetic guide field enables advantageous frequency scaling without deployment constraints, making them especially attractive for high-impact applications in fusion science, turbulence diagnostics, non-destructive testing and biochemical spectroscopy. The novel energy recovery techniques presented in this paper have broad applicability to many electron-beam driven devices, bringing revolutionary potential to future THz source technologies.
Latin American adults who regularly use macrodoses of psychedelics: a cross-sectional study
Authors: Véliz-García Oscar , Domic-Siede Marcos 
Published: October 2024

AbstractPsychedelics have a complex history marked by traditional use among indigenous cultures, early scientific interest, and subsequent prohibition. Despite their classification as controlled substances, recent decades have witnessed a resurgence of research into their therapeutic potential for various mental health conditions. However, most studies have focused on controlled clinical settings, leaving a significant gap in understanding how these substances are used in naturalistic contexts, particularly in Latin America. This study investigates the regular use of macrodoses of psychedelics among Latin American adults. We aimed to characterize the sociodemographic profiles, consumption practices, and subjective effects experienced by individuals who use psychedelics regularly. Data were collected via an online survey from 4,270 participants across several Latin American countries. Results indicated a diverse user base with varied motivations, predominantly psychological and spiritual well-being. The most frequently used substance was psilocybin mushrooms, with significant associations found between demographic variables and specific psychedelics used. The study provides new insights into the naturalistic use of psychedelics in Latin America, highlighting the need for informed, safe, and legal use frameworks.
Assessment of a LPG hybrid solar dryer assisted with smart air circulation system for drying basil leaves
Authors: Khater El-Sayed Gomaa , Bahnasawy Adel Hamed , Oraiath Awad Ali Tayoush , Alhag Sadeq K. , Al-Shuraym Laila A. , Moustapha Moustapha Eid , Elwakeel Abdallah Elshawadfy , Elbeltagi Ahmed , Salem Ali , Metwally Khaled A. , Abdalla Mohamed A. I. , Hussein Mahmoud M. , Abdeen Mohamed Anwer 
Published: October 2024

AbstractThe fluctuation of solar radiation throughout the day presents a significant obstacle to the widespread adoption of solar dryers for the dehydration of agricultural products, particularly those that are sensitive to high temperatures, such as basil leaf drying during the winter season. Consequently, this recent study sought to address the limitations of solar-powered dryers by implementing a hybrid drying system that harnesses both solar energy and liquid petroleum gas (LPG). Furthermore, an innovative automatic electronic unit was integrated to facilitate the circulation of air between the drying chamber and the ambient environment. Considering the solar radiation status in Egypt, an LPG hybrid solar dryer has been developed to be suitable for both sunny and cloudy weather conditions. This hybrid solar dryer (HSD) uses indirect forced convection and a controlled auxiliary heating system (LPG) to regulate both temperature and relative humidity, resulting in increased drying rates, reduced energy consumption, and the production of high-quality dried products. The HSD was tested and evaluated for drying basil leaves at three different temperatures of50, 55, and 60 °C and three air changing rates of 70, 80, and 90%, during both summer and winter sessions. The obtained results showed that drying basil at a temperature of 60 °C and an air changing rate of 90% led to a decrease in the drying time by about 35.71% and 35.56% in summer and winter, respectively, where summer drying took 135–210 min and winter drying took 145–225 min to reach equilibrium moisture content (MC). Additionally, the effective moisture diffusivity ranged from 5.25 to 9.06 × 10− 9 m2/s, where higher values of effective moisture diffusivity (EMD) were increased with increasing both drying temperatures and air change rates. Furthermore, the activation energy decreased from 16.557 to 25.182 kJ/mol to 1.945–15.366 kJ/mol for the winter and summer sessions, respectively. On the other hand, the analysis of thin-layer kinetic showed that the Modified Midilli II model has a higher coefficient of determination R2, the lowest χ2, and the lowest root mean square error (RMSE) compared to the other models of both winter and summer sessions. Finally, the LPG hybrid solar dryer can be used for drying a wide range of agricultural products, and it is more efficient for drying medicinal plants. This innovative dryer utilizes a combination of LPG and solar energy, making it efficient and environmentally friendly.
Validation and comparison of virtual reality and 3D mobile games for cognitive assessment against ACE-III in 82 young participants
Authors: Bhargava Yesoda , Kottapalli Ashwani , Baths Veeky 
Published: October 2024

AbstractCurrent medical and clinical ecosystem for dementia detection is inadequate for its early detection. Traditional cognitive assessments are introduced after cognitive impairment has begun to disrupt the real-world functioning of the person. Moreover, these tools are paper-pen based and fail to replicate the real-world situations wherein the person ultimately lives, acts and grows. The lack of tools for early detection of dementia, combined with absence of reliable pharmacological cure compound the problems associated with dementia diagnosis and care. Advancement of technology has facilitated early prediction of disease like cancer, diabetes, heart disease, but hardly any such translation has been observed for dementia or cognitive impairment. Given this background, we examine the potential of Virtual Reality (VR) and 3D Mobile-based goal-oriented games for cognitive assessment. We evaluate three games (2 in VR, one in mobile) among 82 young participants (aged 18–28 years) and compare and contrast the game-based results with their Addenbrooke Cognitive Examination (ACE-III) scores. Three main analysis methods are used: Correlative, Z-score and Regression analysis. Positive correlation was observed for ACE-III and game-based scores. Z-scores analysis revealed no difference between the two scores, and stronger statistical significance was found between game scores and cognitive health factors like age, smoking compared to ACE-III. Specific game performances also revealed about real-world traits of participants, like hand-use confusion and direction confusion. Results establish the plausibility of using goal-oriented games for more granular, time-based, and functional cognitive assessment.
Metabolomic changes in preterminal serum samples of rhesus macaques exposed to two different lethal doses of total-body gamma-radiation
Authors: Carpenter Alana D. , Empfield Keirstyn M. , Petrus Sarah A. , Fatanmi Oluseyi O. , Wise Stephen Y. , Tyburski John B. , Cheema Amrita K. , Singh Vijay K. 
Published: October 2024

AbstractExposure to ionizing radiation induces cellular and molecular damage leading to a cascade of events resulting in tissue and organ injury. Our study strives to characterize and validate metabolomic changes in preterminal stage (immediately prior to death) samples collected from rhesus macaques lethally irradiated with one of two different doses of radiation. Peripheral blood samples were collected pre-exposure, post-exposure, and at the preterminal stage of nonhuman primates (NHPs that did not survive exposure with 7.2 Gy or 7.6 Gy total-body radiation (LD60-80/60)). We analyzed global metabolomic alterations using ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (QTOF-MS) in serum samples collected at various timepoints in relation to radiation exposure. The goal of this study was to validate the metabolic shifts present in samples collected just prior to death, which were reported earlier in a preliminary study with a limited number of samples and a single dose of radiation. Here, we demonstrate that radiation exposure induced significant time-dependent metabolic alterations compared with pre-exposure samples. We observed significant metabolite dysregulation in animals exposed to 7.6 Gy compared to 7.2 Gy. Greater metabolic disruption was observed in the preterminal groups than all of the other post-irradiation timepoints in both cohorts. Metabolomic shifts in these preterminal groups also revealed consistent disturbances in sphingolipid metabolism, steroid hormone biosynthesis, and glycerophospholipid metabolism pathways. Overall, the sphingolipid metabolism pathway appears to be representative of the preterminal phenotype, confirming the results of our preliminary study. These results offer important and novel insights for identification and validation of biomarkers for lethality, and such observations would be valuable for triage during a radiological/nuclear mass casualty scenario.
Validity of GPS data in driving cycles
Authors: Smith Harry , Akhtar Suhail , Caulfield Brian , O'Mahony Margaret 
Published: October 2024

AbstractThere is continuous research into driving cycles (DCs) as researchers across the globe seek to define driving characteristics, energy consumption, and emissions in a local context. For decades, data collection for the development of DCs has been conducted in three ways: chase car, instrumented vehicle, or a combination of both. Many studies have moved on to cheap and easily available global positioning system (GPS) technology, while others record vehicle data directly through the on‐board diagnostics (OBD) port. However, there are major limitations to GPS data collection such as frequent inaccuracies and loss of coverage in urban environments. For this reason, both OBD and GPS vehicle speed data have been collected. Then, the recorded data has been analysed to capture any differences in sampling rates and dropping data. Finally, basic DCs were created from smoothed GPS and OBD data and compared. DCs were developed with a microtrip‐based method, and a relative error term was used to compare candidate DCs to the collected data. DCs were compared based on kinematic characteristic parameters that are most used in the field. The results of this study could be used to assess the validity of GPS‐based DCs compared to OBD cycles using low‐cost devices.
Green synthesis of cerium oxide nanoparticles via Linum usitatissimum seeds extract and assessment of its biological effects
Authors: Foroutan Zahra , Sabouri Zahra , Afshari Amir Reza , Mostafapour Asma , Far Bahareh Farasati , Jalili‐Nik Mohammad , Darroudi Majid 
Published: October 2024

AbstractIn the current work, CeO2‐NPs were produced via a green chemistry process via Linum usitatissimum seeds extract as the stabilizing agent. The CeO2‐NPs were characterized by the results of analytical techniques such as TGA/DTA, FTIR, UV–vis, XPS, XRD, and FESEM/PSA/EDX. The outcomes of the XRD pattern affirmed the crystalline structure of this product with the suggested size of about 20 nm, while the FESEM/PSA pictures presented spherical morphology. Additionally, the XPS analysis of CeO2‐NPs affirmed the presence of a Ce+3 oxidation state in the sample, as well as Ce+4 as the major valence of cerium oxide. The results of the MTT test on Huh‐7 cancer cells and HFF normal cells displayed the lack of any significant cytotoxicity even at concentrations up to 1000 µg/mL. Therefore, the obtained synthesized CeO2‐NPs through this green method could be suggested as a prospective agent for medical applications due to its non‐toxic effects.
A non‐isolated single‐input dual‐output DC–DC boost converter with high‐voltage gain
Authors: Abbasi Saeed , Nahavandi Ali 
Published: October 2024

AbstractThis paper presents a new structure of a high step‐up DC–DC converter. The proposed converter has one input and two outputs with different voltage levels, suitable for applications that require multiple voltage levels such as photovoltaic systems, electric vehicles and DC microgrids. The proposed converter is expandable to more outputs and has a higher voltage conversion ratio compared to similar converters. The operational modes of the proposed converter are investigated and the conversion ratio of the converter is obtained. Additionally, small‐signal equations of the converter are derived, and using state‐space matrices, transfer functions of the converter are obtained and the controller is designed. Also, the proposed converter is compared with similar converters and power losses equations of the converter are derived. To investigate the converter's performance, simulations are done using MATLAB/Simulink software. Moreover, to validate the theoretical analysis, a laboratory prototype is implemented. The obtained results confirm the operation of the proposed converter.
Active protection scheme based on high‐frequency current for distribution networks with inverter‐interfaced distributed generators
Authors: Li Haifeng , Liang Huamin , Wang Zhidong , Zhang Zhenggang , Liang Yuansheng 
Published: October 2024

AbstractWith the high penetration and flexible access of inverter‐interfaced distributed generators (IIDGs), it is gradually becoming difficult for traditional protection schemes to meet the requirements for the safe operation of distribution networks (DNs). Active protection schemes based on power electronic equipment provide a new approach. On the basis of the controllability of voltage source converters, a method for active high‐frequency signal injection and a selection principle for the corresponding control parameters are proposed. Considering the impact of T‐connected load branches on the protected line, the high‐frequency current characteristics at the three terminals during internal and external faults are analysed. On this basis, an active protection scheme based on high‐frequency current is proposed for DNs with IIDGs. The performance of the proposed scheme is verified via PSCAD/EMTDC simulation software. The test results show that the proposed scheme can reliably trip during internal faults and identify faulty phases, which has better endurance to fault resistance.