bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drivers of perceived market and eco-performance in the foodservice industry
Authors: Kim Min-Seong , Thapa Brijesh , Holland Stephen 
Published: January 2018

PurposeTo foster environmental and sustainable practices, foodservice enterprises should exhibit a high degree of corporate social responsibility (CSR), ecological consciousness and innovation in green initiatives. This study examines the influences of CSR practices (i.e. economic, legal, ethical and philanthropic), environmental marketing activities (i.e. strategic and tactical) and innovation (i.e.technological and organizational) as drivers of market and eco-performance in the foodservice industry.Design/methodology/approachBased on an established framework of CSR, environmental marketing and innovation, an exploratory conceptual model was formulated and empirically assessed. Survey data were collected from representatives of the Korean foodservice franchise industry. Data analysis consisted of frequency analysis, reliability analysis, confirmatory factor analysis, correlation analysis and path analysis.FindingsMarket performance was influenced by tactical environmental marketing, as well as technological and organizational innovation. Eco-performance was affected by tactical environmental marketing and technological innovation. However, technological innovation and organizational innovation were influenced by tactical environmental marketing, but were not significantly affected by strategic environmental marketing. Additionally, strategic environmental marketing was influenced by economic, legal, ethical and philanthropic CSR. Last, tactical environmental marketing was affected by economic, ethical and philanthropic CSR.Practical implicationsCSR, environmental marketing activities and innovation provide numerous benefits to businesses. Such benefits include enhanced market and eco-performance, both of which create competitive advantages.Originality/valueThere is a paucity of research on the effects of environmental marketing and innovation on corporate performance in the foodservice industry. The findings provide greater insights into the impacts of CSR, environmental marketing and innovation on corporations’ desired outcomes.
Official sports sponsorship fortress vs ambush marketing attack
Authors: Schmidt Steffen , Limbach Matthias , Langner Sascha , Wiedmann Klaus-Peter , Albertsen Levke , Reiter Philipp 
Published: January 2018

Purpose
The purpose of this paper is to assess the effectiveness of event-related sports sponsorship and ambushing activity using social media video advertising that aim to affect spectators’ implicit and explicit brand information processing.

Design/methodology/approach
A dual model of brand knowledge is used that considers the implicit and explicit information processing of marketing-induced brand messages. A web study was conducted prior to the 2014 FIFA World Cup. Each participant implicitly and explicitly evaluated either one sponsor brand or one ambush brand before and after watching the video advertisement (within-subject design). A Wilcoxon signed-rank test was used to evaluate each change of the pre-post testing scores.

Findings
Implicit and explicit brand associations as well as brand behavior were partially affected by the short contact with the advertisements of sponsor brands and ambush brands. In this regard, the implicit association measurements were more sensitive to reveal changes in the brand knowledge structure than their explicit counterparts. Furthermore, sponsorship advertising was slightly more effective than ambush advertising.

Originality/value
The current exploratory study evaluated for the first time the performance of event-related video advertisements that were originally released on social media of sponsor brands and ambush brands. The findings emphasize the necessary requirement of evaluating the implicit processing in addition to the explicit processing of sponsorship information to ensure a holistic evaluation of consumers’ memory with regard to the effectiveness of a sponsorship activity.
Supporting the employability agenda in university libraries
Authors: Mawson Maria , Haworth Amy C. 
Published: January 2018

Purpose
This paper aims to outline work to support the employability agenda in the Library at the University of Sheffield, set in the context of debates about the nature of employability, employability skills and information literacy in the workplace.

Design/methodology/approach
The paper starts with a brief review of literature on employability and student skills in the UK higher education sector, the place of information literacy as an employability attribute and information literacy in the workplace. It goes on to outline work done in the Library at the University of Sheffield to support the employability agenda. This includes the development of a commercial awareness workshop in collaboration with other services and the incorporation of student and alumni voices in an employability guide.

Findings
The literature reviewed highlights the differences between information literacy in the workplace and academia. This could present challenges and opportunities in promoting information literacy as an employability attribute. The case study highlights the benefits of working in collaboration with students and services beyond the library in the employability arena.

Originality/value
The approaches taken in Sheffield may be of interest to other institutions looking to develop support for the employability agenda.
Improvement of Doppler measurement using multiple-input multiple-output (MIMO) concept in radar-based automotive sensor detecting pedestrians
Authors: Mohd Basir Shafinaz , Pasya Idnin , Yaakob Tajmalludin , Abd Rashid Nur Emileen , Kobayashi Takehiko 
Published: January 2018

Purpose
This paper aims to present an approach of utilizing multiple-input multiple-output (MIMO) radar concept to enhance pedestrian classification in automotive sensors. In a practical environment, radar signals reflected from pedestrians and slow-moving vehicles are similar in terms of reflecting angle and Doppler returns, inducing difficulty for target discrimination. An efficient discrimination between the two targets depends on the ability of the sensor to extract unique characteristics from each target, for example, by exploiting Doppler signatures. This study describes the utilization of MIMO radar for Doppler measurement and demonstrates its application to improve pedestrian classification through actual laboratory measurements.

Design/methodology/approach
Multiple non-modulated sinusoidal signals are transmitted orthogonally over a MIMO array using time division scheme, illuminating human and non-human targets. The reflected signal entering each of the receiving antenna are combined at the radar receiver prior to Doppler processing. Doppler histogram was formulated based on a series of measurements, and the Doppler spread of the targets was determined from the histograms. Results were compared between MIMO and conventional single antenna systems.

Findings
Measurement results indicated that the MIMO configuration provides able to capture more Doppler information compared to conventional single antenna systems, enabling a more precise discrimination between pedestrian and other slow-moving objects on the road.

Originality/value
The study demonstrated the effectiveness of using MIMO configuration in radar-based automotive sensor to enhance the accuracy of Doppler estimation, which is seldom highlighted in literature of MIMO radars. The result also indicated its usefulness in improving target discrimination capability of the radar, through actual measurement.
Medical Statistics from Hospitals Establishments of Subdivisions of Military Troops from Composition of the Eastern Rumelian Militias, 1879. General Notes.
Authors: Kyurkchiev Nikolay V. 
Published: January 2018

We note that the first official census in Eastern Rumelia, dates from 1884 and official statistics (including medical statistics) was born much later.In this sense, we hope that our research related to the study of basic classes of diseases medications for treating them and most importantly - the application of modern methods of analysis and statistics regarding frequency of disease, relative share of diseases hospital mortality and others forВ hospitals, subject to the Eastern Rumelian Militias (May -July 1879) may be useful for researchers working on these still not sufficiently researched topics "Health in Eastern Rumelia. Medical statistics".
High-Content Screening of the Medicines for Malaria Venture Pathogen Box for Plasmodium falciparum Digestive Vacuole-Disrupting Molecules Reveals Valuable Starting Points for Drug Discovery
Authors: Tong Jie Xin , Chandramohanadas Rajesh , Tan Kevin Shyong-Wei 
Published: January 2018

ABSTRACT

Plasmodium falciparum
infections leading to malaria have severe clinical manifestations and high mortality rates. Chloroquine (CQ), a former mainstay of malaria chemotherapy, has been rendered ineffective due to the emergence of widespread resistance. Recent studies, however, have unveiled a novel mode of action in which low-micromolar levels of CQ permeabilized the parasite's digestive vacuole (DV) membrane, leading to calcium efflux, mitochondrial depolarization, and DNA degradation. These phenotypes implicate the DV as an alternative target of CQ and suggest that DV disruption is an attractive target for exploitation by DV-disruptive antimalarials. In the current study, high-content screening of the Medicines for Malaria Venture (MMV) Pathogen Box (2015) was performed to select compounds which disrupt the DV membrane, as measured by the leakage of intravacuolar Ca
2+
using the calcium probe Fluo-4 AM. The hits were further characterized by hemozoin biocrystallization inhibition assays and dose-response half-maximal (50%) inhibitory concentration (IC
50
) assays across resistant and sensitive strains. Three hits, MMV676380, MMV085071, and MMV687812, were shown to demonstrate a lack of CQ cross-resistance in parasite strains and field isolates. Through systematic analyses, MMV085071 emerged as the top hit due to its rapid parasiticidal effect, low-nanomolar IC
50
, and good efficacy in triggering DV disruption, mitochondrial degradation, and DNA fragmentation in
P. falciparum
. These programmed cell death (PCD)-like phenotypes following permeabilization of the DV suggests that these compounds kill the parasite by a PCD-like mechanism. From the drug development perspective, MMV085071, which was identified to be a potent DV disruptor, offers a promising starting point for subsequent hit-to-lead generation and optimization through structure-activity relationships.
Lipidomics of Thalassiosira pseudonana under Phosphorus Stress Reveal Underlying Phospholipid Substitution Dynamics and Novel Diglycosylceramide Substitutes
Authors: Hunter Jonathan E. , Brandsma Joost , Dymond Marcus K. , Koster Grielof , Moore C. Mark , Postle Anthony D. , Mills Rachel A. , Attard George S. 
Published: January 2018

ABSTRACT

Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom
Thalassiosira pseudonana
, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. Moreover, the behavior of lipids outside the common headgroup classes and the relationship between lipid substitution and cellular particulate organic P (POP) have yet to be reported. We investigated these through the mass spectrometric lipidomics of P-replete (P
+
) and P-depleted (P
−
)
T. pseudonana
cultures. Nonlipidic POP was depleted rapidly by the initiation of P stress, followed by the cessation of P-lipid biosynthesis and per-cell reductions in the P-lipid levels of successive generations. Minor P-lipid degradative breakdown was observed, releasing P for other processes, but most P-lipids remained intact. This may confer an advantage on efficient heterotrophic lipid consumers in P-limited oceans. Glycerophosphatidylcholine (PC), the predominant P-lipid, was similar in composition to its betaine substitute lipid. During substitution, PC was less abundant per cell and was more highly unsaturated in composition. This may reflect underlying biosynthetic processes or the regulation of membrane biophysical properties subject to lipid substitution. Finally, levels of several diglycosylceramide lipids increased as much as 10-fold under P stress. These represent novel substitute lipids and potential biomarkers for the study of P limitation
in situ
, contributing to growing evidence highlighting the importance of sphingolipids in phycology. These findings contribute much to our understanding of P-lipid substitution, a powerful and widespread adaptation to P limitation in the oligotrophic ocean.

IMPORTANCE
Unicellular organisms replace phosphorus (P)-containing membrane lipids with non-P substitutes when P is scarce, allowing greater growth of populations. Previous research with the model diatom species
Thalassiosira pseudonana
grouped lipids by polar headgroups in their chemical structures. The significance of the research reported here is threefold. (i) We described the individual lipids within the headgroups during P-lipid substitution, revealing the relationships between lipid headgroups and hinting at the underlying biochemical processes. (ii) We measured total cellular P, placing P-lipid substitution in the context of the broader response to P stress and yielding insight into the implications of substitution in the marine environment. (iii) We identified lipids previously unknown in this system, revealing a new type of non-P substitute lipid, which is potentially useful as a biomarker for the investigation of P limitation in the ocean.
Coxiella burnetii Inhibits Neutrophil Apoptosis by Exploiting Survival Pathways and Antiapoptotic Protein Mcl-1
Authors: Cherla Rama , Zhang Yan , Ledbetter Lindsey , Zhang Guoquan 
Published: January 2018

ABSTRACTOur previous study demonstrated that neutrophils play an important role in host defense againstCoxiella burnetiiinfection in mice. In this study, avirulent strainC. burnetiiNine Mile phase II (NMII) was used to examine ifC. burnetiican modulate mouse bone marrow-derived neutrophil apoptosis. The results indicated that NMII can inhibit neutrophil apoptosis. Western blotting demonstrated that caspase-3 cleavage was decreased in NMII-infected neutrophils, while phosphorylated mitogen-activated protein kinase (MAPK) p38 and extracellular signal-regulated kinase 1 (Erk1) were increased. Additionally, p38, Erk1/2, phosphoinositide 3-kinase (PI3K), or NF-κB inhibitors reduced the ability of NMII to inhibit neutrophil apoptosis. These results suggest that NMII-mediated inhibition of neutrophil apoptosis depends on its ability to activate neutrophil MAPK pathways. Antiapoptotic protein myeloid cell leukemia-1 (Mcl-1) was significantly increased in NMII-infected neutrophils, and an Mcl-1 inhibitor significantly reduced the ability of NMII to inhibit neutrophil apoptosis. Mcl-1 protein stability was enhanced by phosphorylation at Thr-163 by Erk, and the protein levels were regulated by p38, Erk, PI3K, and NF-κB. Furthermore, the observation that a type IV secretion system (T4SS)-deficientdotAmutant showed a significantly reduced ability to inhibit neutrophil apoptosis compared to wild-type (WT) NMII suggests that T4SS-secreted factors may be involved in NMII-induced inhibition of neutrophil apoptosis. Collectively, these results demonstrate that NMII inhibits neutrophil apoptosis through inhibition of caspase-3 cleavage and activation of MAPK survival pathways with subsequent expression and stabilization of antiapoptotic protein Mcl-1, a process that may partially require the T4SS.
The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance
Authors: Stellrecht Kathleen A. 
Published: January 2018

ABSTRACT
Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 years, subtype H3N2 viruses evolved to viral clades with mutations in the WHO-recommended target region, such that almost all isolates worldwide have significantly reduced sensitivities with many commercial reverse transcription-PCR tests.
Development and Evaluation of a Broad Bead-Based Multiplex Immunoassay To Measure IgG Seroreactivity against Human Polyomaviruses
Authors: Kamminga Sergio , van der Meijden Els , Wunderink Herman F. , Touzé Antoine , Zaaijer Hans L. , Feltkamp Mariet C. W. 
Published: January 2018

ABSTRACT

The family of polyomaviruses, which cause severe disease in immunocompromised hosts, has expanded substantially in recent years. To accommodate measurement of IgG seroresponses against all currently known human polyomaviruses (HPyVs), including the Lyon IARC polyomavirus (LIPyV), we extended our custom multiplex bead-based HPyV immunoassay and evaluated the performance of this pan-HPyV immunoassay. The VP1 proteins of 15 HPyVs belonging to 13
Polyomavirus
species were expressed as recombinant glutathione
S
-transferase (GST) fusion proteins and coupled to fluorescent Luminex beads. Sera from healthy blood donors and immunocompromised kidney transplant recipients were used to analyze seroreactivity against the different HPyVs. For BK polyomavirus (BKPyV), the GST-VP1 fusion protein-directed seroresponses were compared to those obtained against BKPyV VP1 virus-like particles (VLP). Seroreactivity against most HPyVs was common and generally high in both test populations. Low seroreactivity against HPyV9, HPyV12, New Jersey PyV, and LIPyV was observed. The assay was reproducible (Pearson's
r
2
> 0.84,
P
< 0.001) and specific. Weak but consistent cross-reactivity between the related viruses HPyV6 and HPyV7 was observed. The seroresponses measured by the GST-VP1-based immunoassay and a VP1 VLP-based enzyme-linked immunosorbent assay were highly correlated (Spearman's ρ = 0.823,
P
< 0.001). The bead-based pan-HPyV multiplex immunoassay is a reliable tool to determine HPyV-specific seroresponses with high reproducibility and specificity and is suitable for use in seroepidemiological studies.
Identification and Characterization of Sindbis Virus RNA-Host Protein Interactions
Authors: LaPointe Autumn T. , Gebhart Natasha N. , Meller Megan E. , Hardy Richard W. , Sokoloski Kevin J. 
Published: January 2018

ABSTRACT

Arthropod-borne viruses, such as the members of the genus
Alphavirus
, are a significant concern to global public health. As obligate intracellular pathogens, RNA viruses must interact with the host cell machinery to establish and complete their life cycles. Despite considerable efforts to define the host-pathogen interactions essential for alphaviral replication, an unbiased and inclusive assessment of alphaviral RNA-protein interactions has not been undertaken. Moreover, the biological and molecular importance of these interactions, in the full context of their molecular function as RNA-binding proteins, has not been fully realized. The data presented here introduce a robust viral RNA-protein discovery method to elucidate the Sindbis virus (SINV) RNA-protein host interface. Cross-link-assisted mRNP purification (CLAMP) assessment revealed an extensive array of host-pathogen interactions centered on the viral RNAs (vRNAs). After prioritization of the host proteins associated with the vRNAs, we identified the site of protein-vRNA interaction by a UV cross-linking and immunoprecipitation sequencing (CLIP-seq) approach and assessed the consequences of the RNA-protein binding event of hnRNP K, hnRNP I, and hnRNP M in regard to viral infection. Here, we demonstrate that mutation of the prioritized hnRNP-vRNA interaction sites effectively disrupts hnRNP-vRNA interaction. Correlating with disrupted hnRNP-vRNA binding, SINV growth kinetics were reduced relative to wild-type parental viral infections in vertebrate and invertebrate tissue culture models of infection. The molecular mechanism leading to reduced viral growth kinetics was found to be dysregulated structural-gene expression. Collectively, this study further defines the scope and importance of the alphavirus host-pathogen vRNA-protein interactions.

IMPORTANCE
Members of the genus
Alphavirus
are widely recognized for their potential to cause severe disease. Despite this recognition, there are no antiviral therapeutics, or safe and effective vaccines, currently available to treat alphaviral infection. Alphaviruses utilize the host cell machinery to efficiently establish and complete their life cycle. However, the extent and importance of host-pathogen RNA-protein interactions are woefully undercharacterized. The efforts detailed in this study fill this critical gap, and the significance of this research is 3-fold. First, the data presented here fundamentally expand the scope and understanding of alphavirus host-pathogen interactions. Second, this study identifies the sites of interaction for several prioritized interactions and defines the contribution of the RNA-protein interaction at the molecular level. Finally, these studies build a strategy by which the importance of the given host-pathogen interactions may be assessed in the future, using a mouse model of infection.