bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrasonographic Assessment of Femoral Cartilage Thickness in Patients With Cerebral Palsy
Authors: Adiguzel Emre , Tok Fatih , Ata Emre , Yaşar Evren , Yılmaz Bilge 
Published: July 2017

AbstractBackgroundCerebral palsy (CP) is one of the most disabling syndromes in children. To our knowledge, there has not yet been any reported evaluation by ultrasonography of the effect of CP on distal femoral cartilage. The value of understanding this effect on cartilage is that sonographic evaluation of cartilage thickness may help physicians to predict the joint health of these children.ObjectiveTo determine whether femoral cartilage thickness in patients with CP is different from that in healthy control subjects.DesignCross‐sectional study.SettingNational tertiary rehabilitation center.PatientsThe study included 40 patients with diplegic CP (23 male and 17 female) and 51 healthy control subjects (29 male and 22 female).MethodsDemographic and clinical characteristics were recorded. Cartilage thicknesses were measured.Main Outcome MeasureCartilage thickness measurements were taken from the medial and lateral condyles, and intercondylar areas of both knees.ResultsBoth groups were similar in terms of age, gender, and weight (P > .05). The mean cartilage thickness measurements of the medial condyle and intercondylar area of knees in the CP group were significantly less than those in the healthy control group (all P < .05). There was moderate negative correlation between age and all femoral cartilage thickness measurements in the CP group. There was no correlation between age and femoral cartilage thickness measurements in the healthy group. There was a negative correlation between Gross Motor Functional Classification System levels and cartilage thickness in the CP group. The highest cartilage thickness measurements were detected in level 1 patients, and the lowest measurements were detected in level 5 patients.ConclusionThis study showed that patients with CP have a thinner femoral cartilage than healthy control subjects. Management of patients with CP should include close surveillance.Level of EvidenceIII
Discrepancy between information provided and information required by emergency physicians for long-term care patients
Authors: Parashar Richa , McLeod Shelley , Melady Don 
Published: July 2017

AbstractObjectivesThe primary objective of this study was to identify information included in long-term care (LTC) transfer documentation and to compare it to the information required by local emergency department (ED) physicians to provide optimal care and make decisions for LTC patients.MethodsA retrospective chart review was conducted for a sample of LTC residents transferred by ambulance to the ED of an academic, tertiary care hospital over a 1-year period. All emergency physicians working at the institution were invited to complete an online questionnaire about information included in LTC transfer documentation and information required by emergency physicians to provide care for LTC patients.ResultsOf the 200 charts reviewed, the most common information transferred to the ED with the LTC patient was the patient’s past medical history (n=184, 92.0%), name of family physician (n=182, 91.0%), a list of known allergies (n=179, 89.5%), the reason for transfer to the ED (n=155, 77.5%), the patient’s emergency contact information (n=152, 76.0%), and medication administration record (n=150, 75.0%). From a physician’s perspective, the most frequently requested pieces of information included reason for transfer, past medical history, cognitive status, advanced directives for level of care and resuscitation, and the patient’s emergency contact information. This information was provided 77.5% (n=155), 92.0% (n=184), 24.0% (n=48), 62.0% (n=124), and 76.0% (n=152) of the time, respectively.ConclusionsOur study demonstrates a clear discrepancy between information provided and information required by emergency physicians for LTC patients. Quality improvement initiatives at the local level may help reduce this discrepancy.
Geochemistry and petrogenesis of the early Palaeozoic appinite-granite complex in the Western Kunlun Orogenic Belt, NW China: implications for Palaeozoic tectonic evolution
Authors: ZHU JIE , LI QIUGEN , CHEN XU , TANG HAOSHU , WANG ZONGQI , CHEN YANJING , LIU SHUWEN , XIAO BING , CHEN JUNLU 
Published: July 2017

AbstractThe Datong pluton, the largest early Palaeozoic granitoid in the Western Kunlun Orogenic Belt (WKOB) in NW China, is a typical appinite-granite complex. It consists of diorites, quartz diorites, monzodiorites, quartz monzodiorites, monzonites, quartz monzonites, syenites, granodiorites and monzogranites. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) zircon U–Pb dating yielded crystallization ages of 459 ± 3 Ma for the quartz monzonites and 452 ± 5 Ma for the monzogranites (Late Ordovician). The rocks possess a wide range of SiO2 (56.0–73.4 wt %), MgO (0.17–4.55 wt %) and Mg no. (25–60), with high K2O (2.83–5.29 wt %) contents, exhibiting high-K calc-alkaline to shoshonitic traits. They are characterized by enrichments in large-ion lithophile elements (LILEs) and light rare Earth elements (LREEs), as well as depletions in high-field-strength elements (HFSEs). The rocks have initial 87Sr/86Sr ratios of 0.7086–0.7185, negative εNd(t) values of –3.72 to –1.79 and εHf(t) values vary from –1.6 to +4.7. These features are modelled to show that they were most likely derived from an enriched lithospheric mantle source and that fractional crystallization with minor crustal contamination was involved in their petrogenetic process. Considering the distribution and chronology of the Palaeozoic intrusions – such as Kegang, Bulong, Qiukesu, Yierba, North Kudi, Dongbake, Buya, Ayilixi and Warengzilafu granitoid plutons with ages of c. 420–530 Ma – in conjunction with the Palaeozoic metamorphic overprinting in the WKOB, we propose a divergent double-subduction model to explain the destruction of the Proto-Tethys Ocean and suggest that the Datong pluton was likely emplaced in a post-collisional setting following the termination of subduction in response to slab break-off.
The role of language production mechanisms in children's sentence repetition: Evidence from an inflectionally rich language
Authors: NAG SONALI , SNOWLING MARGARET J. , MIRKOVIĆ JELENA 
Published: July 2017

ABSTRACTWe examine the role of language production mechanisms in sentence repetition, a task widely used as a diagnostic tool in developmental disorders. We investigate sentence repetition in 5- to 8-year-old native speakers of Kannada, an inflectionally rich language of India. The inflectional characteristics of the language make it an ideal testing ground for exploring the engagement of grammatical and phonological encoding processes. We presented active, passive, and embedded sentences and, in a subset of the material, we also manipulated sentence length. Using accuracy and speech error analyses at the sentence, word, and affix levels, we provide evidence that individual differences in task performance are influenced by the linguistic properties of the material. These findings clarify the role of key language production mechanisms involved in sentence repetition. We propose that it is the versatility to develop a profile across several language production mechanisms that makes sentence repetition particularly useful as a clinical tool.
Prospects for Cross-Strait Political Negotiation: Exploring Win-Sets
Authors: Wenger Josh , Chen Ming-tong 
Published: July 2017

AbstractThis study adopts a pre-negotiation approach based on Robert Putnam's win-set concept to examine domestic constraints on cross-Strait political negotiation. Survey research of elite opinion in both China and Taiwan and of public opinion in Taiwan is used to estimate each side's win-set (that is, the set of political negotiation outcomes that could win majority approval domestically) during Ma Ying-jeou's second presidential term in Taiwan (2012–2016). The possibility for overlap in win-sets that could provide a zone of possible agreement and the potential for coalitions in favour of negotiation are analysed. The study finds no win-set overlap and limited potential for coalitions favouring negotiation outcomes with the least distance from overlap, concluding that domestic conditions for formal political negotiations between Beijing and Taipei are unlikely to be ripe in the near term.
Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment
Authors: Li Xiao-Chen , Guo Qing , Zhu Hai-Ying , Jin Long , Zhang Yu-Chen , Zhang Guang-Lei , Xing Xiao-Xu , Xuan Mei-Fu , Luo Qi-Rong , Luo Zhao-Bo , Wang Jun-Xia , Cui Cheng-Du , Li Wen-Xue , Cui Zheng-Yun , Yin Xi-Jun , Kang Jin-Dan 
Published: July 2017

SummaryWe examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P < 0.05). The blastocyst formation rate was higher in the group treated with AZD5438 for 4 h than in the groups treated with AZD5438 for 2 or 6 h (42.8% vs. 38.6% and 37.2%, respectively; P > 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype. The blastocyst formation rate of PA and somatic cell nuclear transfer (SCNT) embryos was similar between oocytes activated by an EP and treated with 2 mM 6-dimethylaminopurine for 4 h and those activated by an EP and treated with 10 µM AZD5438 for 4 h (11.11% vs. 13.40%, P > 0.05). In addition, the level of maturation-promoting factor (MPF) was significantly decreased in oocytes activated by an EP and treated with 10 µM AZD5438 for 4 h. Finally, the mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) and pluripotency-related genes (Oct4, Nanog, and Sox2) were checked by RT-PCR; however, there were no differences between the AZD5438-treated and non-treated control groups. Our results demonstrate that porcine oocyte activation via an EP in combination with AZD5438 treatment can lead to a high blastocyst formation rate in PA and SCNT experiments.
The development of activatable lytic peptides for targeting triple negative breast cancer
Authors: Zhao Hui , Qin Xuan , Yang Dan , Jiang Yanhong , Zheng Weihao , Wang Dongyuan , Tian Yuan , Liu Qisong , Xu Naihan , Li Zigang 
Published: July 2017

AbstractCytolytic peptides are an emerging class of promising cancer therapeutics shown to overcome drug resistance. They eliminate cancer cells via disruption of the phospholipid bilayer of cell membranes, a mechanism that differentiates it from traditional treatments. However, applications of lytic peptides via systematic administration are hampered by nonspecific toxicity. Here, we describe activatable, masked lytic peptides that are conjugated with anionic peptides via a cleavable linker sensitive to matrix metalloproteinases (Ac-w-βA-e8-XPLG*LAG-klUklUkklUklUk-NH2; lower case letters in the sequences represent D-amino-acids, U=Aib, α-aminoisobutyric acid, *cleavage site). The peptides were activated upon being introduced into the triple negative breast cancer cell line MDA-MB-231, which overexpresses secreted matrix metalloproteinases, to selectively cleave the peptide linker. Our results indicate that the activatable design could be applied to improve the targeting ability of lytic peptides.
High-aspect-ratio nanoimprint process chains
Authors: Cadarso Víctor J. , Chidambaram Nachiappan , Jacot-Descombes Loïc , Schift Helmut 
Published: July 2017

AbstractDifferent methods capable of developing complex structures and building elements with high-aspect-ratio nanostructures combined with microstructures, which are of interest in nanophotonics, are presented. As originals for subsequent replication steps, two families of masters were developed: (i) 3.2 μm deep, 180 nm wide trenches were fabricated by silicon cryo-etching and (ii) 9.8 μm high, 350 nm wide ridges were fabricated using 2-photon polymerization direct laser writing. Both emerging technologies enable the vertical smooth sidewalls needed for a successful imprint into thin layers of polymers with aspect ratios exceeding 15. Nanoridges with high aspect ratios of up to 28 and no residual layer were produced in Ormocers using the micromoulding into capillaries (MIMIC) process with subsequent ultraviolet-curing. This work presents and balances the different fabrication routes and the subsequent generation of working tools from masters with inverted tones and the combination of hard and soft materials. This provides these techniques with a proof of concept for their compatibility with high volume manufacturing of complex micro- and nanostructures.
Acylated heptapeptide binds albumin with high affinity and application as tag furnishes long-acting peptides
Authors: Zorzi Alessandro , Middendorp Simon J. , Wilbs Jonas , Deyle Kaycie , Heinis Christian 
Published: July 2017

AbstractThe rapid renal clearance of peptides in vivo limits this attractive platform for the treatment of a broad range of diseases that require prolonged drug half-lives. An intriguing approach for extending peptide circulation times works through a ‘piggy-back’ strategy in which peptides bind via a ligand to the long-lived serum protein albumin. In accordance with this strategy, we developed an easily synthesized albumin-binding ligand based on a peptide-fatty acid chimera that has a high affinity for human albumin (Kd=39 nM). This ligand prolongs the elimination half-life of cyclic peptides in rats 25-fold to over seven hours. Conjugation to a peptide factor XII inhibitor developed for anti-thrombotic therapy extends the half-life from 13 minutes to over five hours, inhibiting coagulation for eight hours in rabbits. This high-affinity albumin ligand could potentially extend the half-life of peptides in human to several days, substantially broadening the application range of peptides as therapeutics.
Essential role of FBXL5-mediated cellular iron homeostasis in maintenance of hematopoietic stem cells
Authors: Muto Yoshiharu , Nishiyama Masaaki , Nita Akihiro , Moroishi Toshiro , Nakayama Keiichi I. 
Published: July 2017

AbstractHematopoietic stem cells (HSCs) are maintained in a hypoxic niche to limit oxidative stress. Although iron elicits oxidative stress, the importance of iron homeostasis in HSCs has been unknown. Here we show that iron regulation by the F-box protein FBXL5 is required for HSC self-renewal. Conditional deletion of Fbxl5 in mouse HSCs results in cellular iron overload and a reduced cell number. Bone marrow transplantation reveals that FBXL5-deficient HSCs are unable to reconstitute the hematopoietic system of irradiated recipients as a result of stem cell exhaustion. Transcriptomic analysis shows abnormal activation of oxidative stress responses and the cell cycle in FBXL5-deficient mouse HSCs as well as downregulation of FBXL5 expression in HSCs of patients with myelodysplastic syndrome. Suppression of iron regulatory protein 2 (IRP2) accumulation in FBXL5-deficient mouse HSCs restores stem cell function, implicating IRP2 as a potential therapeutic target for human hematopoietic diseases associated with FBXL5 downregulation.
Aspartate β-hydroxylase disrupts mitochondrial DNA stability and function in hepatocellular carcinoma
Authors: Tang C , Hou Y , Wang H , Wang K , Xiang H , Wan X , Xia Y , Li J , Wei W , Xu S , Lei Z , Pawlik T M , Wang H , Wu M , Shen F 
Published: July 2017

AbstractThe mechanism of aberrant mitochondrial genome and function in hepatocellular carcinoma (HCC) remains largely unknown. Our previous study demonstrated an increased expression of aspartate β-hydroxylase (ASPH) in HCC tissues, which was associated with tumor invasiveness and a worse prognosis. Currently, we unexpectedly observed the presence of ASPH in purified mitochondrial protein fraction. In addition, immunostaining of both exogenously and endogenously expressed ASPH showed a colocalization with mitochondrial biomarkers. This study aimed to investigate whether the mitochondrial ASPH is involved in mitochondrial malfunction in HCC. Our results showed that ASPH overexpression in HCC tissues was correlated with decreased copy numbers of displacement loop (D-loop) and NADH dehydrogenase subunit 1 (ND-1) and enhanced D-loop mutation, suggesting the disrupted mitochondrial DNA (mtDNA) stability. The reduced mtDNA copy numbers were associated with aggressive clinicopathological features of HCC. The loss of mtDNA integrity induced by enforced expression of ASPH was accompanied with mitochondrial dysfunction, which was characterized by the aberrant mitochondrial membrane potential, decreased ATP generation and enhanced reactive oxygen species. In contrast, knocking down ASPH by siRNA in HCC cell lines showed the opposite impact on mtDNA integrity and function. Mass spectrometry and co-immunoprecipitation further identified that ASPH interacted with histone H2A member X (H2AX). ASPH overexpression diminished the interaction between H2AX and mitochondrial transcription factor A (mtTFA), an important DNA-binding protein for mtDNA replication, which then reduced the binding of mtTFA to D-loop region. Collectively, our results demonstrate that ASPH overexpression disrupts the mtDNA integrity through H2AX–mtTFA signal, thereby affecting mitochondrial functions in HCC.
Piezomagnetism and magnetoelastic memory in uranium dioxide
Authors: Jaime M. , Saul A. , Salamon M. , Zapf V. S. , Harrison N. , Durakiewicz T. , Lashley J. C. , Andersson D. A. , Stanek C. R. , Smith J. L. , Gofryk K. 
Published: July 2017

AbstractThe thermal and magnetic properties of uranium dioxide, a prime nuclear fuel and thoroughly studied actinide material, remain a long standing puzzle, a result of strong coupling between magnetism and lattice vibrations. The magnetic state of this cubic material is characterized by a 3-k non-collinear antiferromagnetic structure and multidomain Jahn-Teller distortions, likely related to its anisotropic thermal properties. Here we show that single crystals of uranium dioxide subjected to strong magnetic fields along threefold axes in the magnetic state exhibit the abrupt appearance of positive linear magnetostriction, leading to a trigonal distortion. Upon reversal of the field the linear term also reverses sign, a hallmark of piezomagnetism. A switching phenomenon occurs at ±18 T, which persists during subsequent field reversals, demonstrating a robust magneto-elastic memory that makes uranium dioxide the hardest piezomagnet known. A model including a strong magnetic anisotropy, elastic, Zeeman, Heisenberg exchange, and magnetoelastic contributions to the total energy is proposed.
Flexible interlocked porous frameworks allow quantitative photoisomerization in a crystalline solid
Authors: Zheng Yongtai , Sato Hiroshi , Wu Pengyan , Jeon Hyung Joon , Matsuda Ryotaro , Kitagawa Susumu 
Published: July 2017

AbstractPhotochromic molecules have shown much promise as molecular components of stimuli-responsive materials, but despite recent achievements in various photoresponsive materials, quantitative conversion in photochemical reactions in solids is hampered by the lack of intrinsic structural flexibility available to release stress and strain upon photochemical events. This issue remains one of the challenges in developing solid-state photoresponsive materials. Here, we report a strategy to realize photoresponsive crystalline materials showing quantitative reversible photochemical reactions upon ultraviolet and visible light irradiation by introducing structural flexibility into crystalline porous frameworks with a twofold interpenetration composed of a diarylethene-based ligand. The structural flexibility of the porous framework enables highly efficient photochemical electrocyclization in a single-crystal-to-single-crystal manner. CO2 sorption on the porous crystal at 195 K is reversibly modulated by light irradiation, and coincident X-ray powder diffraction/sorption measurements clearly demonstrate the flexible nature of the twofold interpenetrated frameworks.
PLATE-Seq for genome-wide regulatory network analysis of high-throughput screens
Authors: Bush Erin C. , Ray Forest , Alvarez Mariano J. , Realubit Ronald , Li Hai , Karan Charles , Califano Andrea , Sims Peter A. 
Published: July 2017

AbstractPharmacological and functional genomic screens play an essential role in the discovery and characterization of therapeutic targets and associated pharmacological inhibitors. Although these screens affect thousands of gene products, the typical readout is based on low complexity rather than genome-wide assays. To address this limitation, we introduce pooled library amplification for transcriptome expression (PLATE-Seq), a low-cost, genome-wide mRNA profiling methodology specifically designed to complement high-throughput screening assays. Introduction of sample-specific barcodes during reverse transcription supports pooled library construction and low-depth sequencing that is 10- to 20-fold less expensive than conventional RNA-Seq. The use of network-based algorithms to infer protein activity from PLATE-Seq data results in comparable reproducibility to 30 M read sequencing. Indeed, PLATE-Seq reproducibility compares favorably to other large-scale perturbational profiling studies such as the connectivity map and library of integrated network-based cellular signatures.
Hypoxia ameliorates intestinal inflammation through NLRP3/mTOR downregulation and autophagy activation
Authors: Cosin-Roger Jesus , Simmen Simona , Melhem Hassan , Atrott Kirstin , Frey-Wagner Isabelle , Hausmann Martin , de Vallière Cheryl , Spalinger Marianne R. , Spielmann Patrick , Wenger Roland H. , Zeitz Jonas , Vavricka Stephan R. , Rogler Gerhard , Ruiz Pedro A. 
Published: July 2017

AbstractHypoxia regulates autophagy and nucleotide-binding oligomerization domain receptor, pyrin domain containing (NLRP)3, two innate immune mechanisms linked by mutual regulation and associated to IBD. Here we show that hypoxia ameliorates inflammation during the development of colitis by modulating autophagy and mammalian target of rapamycin (mTOR)/NLRP3 pathway. Hypoxia significantly reduces tumor necrosis factor α, interleukin (IL)-6 and NLRP3 expression, and increases the turnover of the autophagy protein p62 in colon biopsies of Crohn’s disease patients, and in samples from dextran sulfate sodium-treated mice and Il-10−/− mice. In vitro, NF-κB signaling and NLRP3 expression are reduced through hypoxia-induced autophagy. We also identify NLRP3 as a novel binding partner of mTOR. Dimethyloxalylglycine-mediated hydroxylase inhibition ameliorates colitis in mice, downregulates NLRP3 and promotes autophagy. We suggest that hypoxia counteracts inflammation through the downregulation of the binding of mTOR and NLRP3 and activation of autophagy.