bluuebunny/crossref_metadata_2025_split huggingface dataset:
An update of the breeding population status of the critically endangered Mariana Crow Corvus kubaryi on Rota, Northern Mariana Islands 2013–2014
Authors: KRONER ANDRIA , HA RENEE ROBINETTE 
Published: March 2017

SummaryThe critically endangered Mariana Crow now exists in a single population on the island of Rota, Northern Mariana Islands. Targeted management requires an accurate measure of the population status of this species. In Mariana Crows the breeding population is both the easiest cohort to accurately survey and the most important segment of the population in terms of population recovery. The total number of Mariana Crow territorial pairs was estimated on the island of Rota using a direct count method, and total population size was calculated using a Chapman estimate. From September 2013 to April 2014, 46 crow pairs were found and up to an additional eight pairs were estimated in unsearched areas. The total population was estimated to be 178 individuals. This represents a 10–23% decline in pairs in the six years since 2007 and a 46–53% decline since 1998. This number is also considerably lower than the minimum 75 pairs recommended to maintain a viable population on Rota.
Adopting new medical technologies in Russian hospitals: what causes inefficiency? (qualitative study)
Authors: Shishkin Sergey , Zasimova Liudmila 
Published: March 2017

AbstractThe adoption of new medical technologies often generates losses in efficiency associated with the excess or insufficient acquisition of new equipment, an inappropriate choice (in terms of economic and clinical parameters) of medical equipment, and its poor use. Russia is a good example for exploring the problem of the ineffective adoption of new medical technologies due to the massive public investment in new equipment for medical institutions in 2006–2013. This study examines the procurement of new technologies in Russian hospitals to find the main causes of inefficiency. The research strategy was based on in-depth semistructured interviews with representatives of prominent actors (regional health care authorities, hospital executives, senior physicians). The main result is that inefficiencies arise from the contradiction between hospitals’ and authorities’ motivation for acquiring new technologies: hospitals tend to adopt technologies which bring benefits to their department heads and physicians and minimize maintenance and servicing costs, while the authorities’ main concern is the initial cost of the technology.
Pantomime mit chronisch kranken Kindern
Authors: Brosig Burkhard , Becker Lena , Kaczmarek-Kolb Anna , Speier Annika , Zimmer Klaus-Peter 
Published: March 2017

Zusammenfassung. Dieser Artikel präsentiert Arbeitskonzept, angenommene Wirkungsweisen und Besonderheiten eines kreativtherapeutischen Ansatzes mit pantomimischem Zugang für Kinder und Jugendliche. Es werden Möglichkeiten erörtert, die Sitzungen mit einem professionellen Pantomimen in den Klinikalltag auf einer allgemeinpädiatrischen Station zu integrieren. Vor dem Hintergrund der bei chronisch kranken Kindern häufig mangelhaft ausgeprägten emotionalen Differenzierungsfähigkeit diskutiert der vorliegende Beitrag Vorteile nonverbaler, pantomimischer Arbeit. Darüber hinaus werden die Ergebnisse einer Patientenbefragung mit n = 94 jungen Patienten und n = 36 Elternteilen zur Zufriedenheit und Akzeptanz des therapeutischen Ansatzes vorgestellt. Die Ergebnisse verdeutlichen, dass die pantomimische Arbeit zur Ergänzung der organmedizinischen Therapie positiv aufgenommen wird.
Impact of Music on Cardiac Patients
Authors: Mastnak Wolfgang 
Published: March 2017

Abstract. Controlled studies and clinical observations can elucidate the influence of music on blood pressure and heart rate, but do not explain underlying mechanisms. Clarification of contradictory scientific results requires an understanding of corresponding psycho-neuroendocrinological processes and is also indispensable to patient-centered cardiac music therapy interventions. The paper is intended to complement the thematic issue on music therapy in cardiology (MTK 25 (1), 2014). To identify neural and neuroendocrine modules linking auditory and neurocardiac systems. This should facilitate a diagnosis-specific application of music for cardiac patients. Review of empirical, neuroanatomic, and interdisciplinary medical findings about cerebral music processing. Meta-synthesis and construction of a theoretical framework that explains the impact of music on blood pressure and heart rate. Music-induced cardiac processes can be elucidated by neural and neuroendocrine modules linking auditory processing areas and neurocardiac centers. Efferent projections from the auditory pathway as well as top–down projections arising from the auditory cortex form specific functional circuitries. Overlapping and interacting projections from cortical and subcortical regions to associative processing organs (e. g., the insular cortex) integrate both physiologically and cognitively processed sound factors (i. e., the dualistic model). Via corresponding modules the auditory system is indirectly linked to hypothalamic nuclei, influencing the hypothalamic–pituitary–adrenal axis as well as vasopressin- and oxytocin-related processes. Connections with the locus coeruleus refer to noradrenergic sound–heart regulation. Physiological mechanisms interacting with individual musical experiences require the synopsis of evidence-based findings, neuroendocrinological explanations, and differential psychological profiles for reliable treatment planning.
Beliefs Are Not Behavior
Authors: Radtke Theda , Scholz Urte 
Published: March 2017

Abstract. Compensatory health beliefs (CHBs), defined as belief that an unhealthy behavior can be compensated by engaging in another healthy behavior, are negatively predictive of health-behavior change intentions and behavior. However, CHBs have to be distinguished from compensatory health behavior (CBs), which is defined as compensatory behavior that an individual engages in. As it has not been investigated to date, the aim of this study was to systematically examine the distinction between CHBs and CBs in the context of alcohol consumption. The baseline sample consisted of 898 participants (mainly students, mean age = 23.57 years). For running exploratory and confirmatory factor analyses on CHBs and CBs, the split-half sample method was used. Moreover, the relationships of CHBs and CBs with health-related variables were assessed by regression analyses. The cross-sectional analyses supported the distinction between CHBs and CBs. In contrast to the CHBs, CBs were positively predictive of the intention to drink less alcohol and alcohol consumption. The consideration of CBs when investigating health behavior is highly relevant.
Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils
Authors: Castro Sowmya A , Collighan Russell , Lambert Peter A , Dias Irundika HK , Chauhan Parbata , Bland Charlotte E , Milic Ivana , Milward Michael R , Cooper Paul R , Devitt Andrew 
Published: March 2017

AbstractPeriodontal disease is a prevalent chronic inflammatory condition characterised by an aberrant host response to a pathogenic plaque biofilm resulting in local tissue damage and frustrated healing that can result in tooth loss. Cysteine proteases (gingipains) from the key periodontal pathogen Porphyromonas gingivalis have been implicated in periodontal disease pathogenesis by inhibiting inflammation resolution and are linked with systemic chronic inflammatory conditions such as rheumatoid arthritis. Efficient clearance of apoptotic cells is essential for the resolution of inflammation and tissue restoration. Here we sought to characterise the innate immune clearance of apoptotic cells and its modulation by gingipains. We examined the capacity of gingipain-treated macrophages to migrate towards and phagocytose apoptotic cells. Lysine gingipain treatment of macrophages impaired macrophage migration towards apoptotic neutrophils. Furthermore, lysine gingipain treatment reduced surface expression levels of CD14, a key macrophage receptor for apoptotic cells, which resulted in reduced macrophage interactions with apoptotic cells. Additionally, while apoptotic cells and their derived secretome were shown to inhibit TNF-α-induced expression by P. gingivalis lipopolysaccharide, we demonstrated that gingipain preparations induced a rapid inflammatory response in macrophages that was resistant to the anti-inflammatory effects of apoptotic cells or their secretome. Taken together, these data indicate that P. gingivalis may promote the chronic inflammation seen in periodontal disease patients by multiple mechanisms, including rapid, potent gingipain-mediated inflammation, coupled with receptor cleavage leading to defective clearance of apoptotic cells and reduced anti-inflammatory responses. Thus, gingipains represent a potential therapeutic target for intervention in the management of chronic periodontal disease.
CASC2c as an unfavorable prognosis factor interacts with miR-101 to mediate astrocytoma tumorigenesis
Authors: Liu Changhong , Sun Yingnan , She Xiaoling , Tu Chaofeng , Cheng Xiping , Wang Lin , Yu Zhibin , Li Peiyao , Liu Qing , Yang Honghui , Li Guiyuan , Wu Minghua 
Published: March 2017

AbstractmiR-101 has been suggested as a tumor suppressor, but the promoter methylation and loss of heterozygosity didn’t contribute to its low expression in astrocytoma. We investigated the role of a new long non-coding RNA CASC2c binding with miR-101. High CASC2c was positively correlated with astrocytoma progression, and an unfavorable prognosis factor for patients. Knockdown CASC2c inhibited proliferation and tumorgenesis. Overexpression of CASC2c promotes the malignant characteristic of astrocytoma cells.CASC2c directly bound miR-101 and mediated pre-miR-101 processing into mature miR-101, and functions as a competitor of miR-101 target genes such as CPEB1. Patients who possessed both low CASC2c and high miR-101 had a longer survival than those of low CASC2c alone or high miR-101 alone. In summary, CASC2c plays the onco-RNA role in the tumorgenesis of astrocytoma by acting as a decoy miR-101 sponge. Combination of low expression of CASC2c and high expression of miR-101 has an important referential significance to evaluate the prognosis of patients.
Nuclear Drosha enhances cell invasion via an EGFR-ERK1/2-MMP7 signaling pathway induced by dysregulated miRNA-622/197 and their targets LAMC2 and CD82 in gastric cancer
Authors: Xu Liyun , Hou Yixuan , Tu Gang , Chen Yanlin , Du Yan-e , Zhang Hailong , Wen Siyang , Tang Xi , Yin Jiali , Lang Lei , Sun Kexin , Yang Guanglun , Tang Xiaoli , Liu Manran 
Published: March 2017

AbstractDrosha is an RNA III-like enzyme that has an aberrant expression in some tumors. Our previous studies showed the aberrant Drosha in gastric tumors. However, the roles of nuclear Drosha, the main regulator of microRNA (miRNA) biogenesis, in gastric cancer (GC) progression remain poorly understood. In this study, we demonstrated that nuclear Drosha is significantly associated with cell invasion of GC and that Drosha silence impedes the tumor invasion. Knockdown of Drosha led to a set of dysregulated miRNAs in GC cells. Multiple targets of these miRNAs were the members in cell migration, invasion and metastasis-associated signaling (e.g. ECM-receptor interaction, focal adhesion, p53 signaling and MAPK signaling pathway) revealed by bioinformatics analysis. LAMC2 (a key element of ECM-receptor signaling) and CD82 (a suppressor of p53 signaling) are the targets of miR-622 and miR-197, respectively. High levels of LAMC2 and low levels of CD82 were significantly related to the worse outcome for GC patients. Furthermore, overexpression of LAMC2 and knockdown of CD82 markedly promoted GC cell invasion and activated EGFR/ERK1/2-MMP7 signaling via upregulation of the expression of phosphorylated (p)-EGFR, p-ERK1/2 and MMP7. Our findings suggest that nuclear Drosha potentially has a role in the development of GC.
Role for RIP1 in mediating necroptosis in experimental intracerebral hemorrhage model both in vivo and in vitro
Authors: Shen Haitao , Liu Chenglin , Zhang Dongping , Yao Xiyang , Zhang Kai , Li Haiying , Chen Gang 
Published: March 2017

AbstractCell death is a hallmark of second brain injury after intracerebral hemorrhage (ICH); however, the mechanism still has not been fully illustrated. In this study, we explored whether necroptosis, a type of regulated necrosis, has an essential role in brain injury after ICH. We found that inhibiting receptor-interacting protein 1 (RIP1) – a core element of the necroptotic pathway – by a specific chemical inhibitor or genetic knockdown attenuated brain injury in a rat model of ICH. Furthermore, necroptosis of cultured neurons could be induced by conditioned medium from microglia stimulated with oxygen hemoglobin, and this effect could be inhibited by TNF-α inhibitor, indicating that TNF-α secreted from activated microglia is an important factor in inducing necroptosis of neurons. Undoubtedly, overexpression of RIP1 increased conditioned medium-induced necroptosis in vitro, but this effect was partially diminished in mutation of serine kinase phosphorylation site of RIP1, showing that phosphorylation of RIP1 is the essential molecular mechanism of necroptosis, which was activated in the in vitro model of ICH. Collectively, our investigation identified that necroptosis is an important mechanism of cell death in brain injury after ICH, and inhibition of necroptosis may be a potential therapeutic intervention of ICH.
Endogenous AMPK acts as a detrimental factor in fulminant hepatitis via potentiating JNK-dependent hepatocyte apoptosis
Authors: Hu Kai , Gong Xianqiong , Ai Qing , Lin Ling , Dai Jie , Cai Lu , Jiang Rong , Ge Pu , Zhang Li 
Published: March 2017

AbstractThe energy sensor AMP-activated protein kinase (AMPK) is crucial for energy homeostasis. Recent studies have revealed that AMPK is involved in various energy-intensive pathological processes such as inflammation and apoptosis. The physiological functions of hepatic AMPK have been well studied, but the pathological significance of AMPK in liver disorders remains largely unknown. In the present study, the phosphorylation status and the roles of AMPK were investigated in mice with lipopolysaccharide (LPS)/d-galactosamine (D-Gal)-induced fulminant hepatitis. The experimental data indicated that the phosphorylation of hepatic AMPK increased in mice with LPS/D-Gal-induced fulminant hepatitis. Pretreatment with the AMPK inhibitor compound C enhanced the early production of pro-inflammatory cytokines but suppressed the late activation of the caspase cascade, reduced the number of TUNEL-positive cells, decreased the elevation of aminotransferases, alleviated the histological abnormalities and improved the survival rate of LPS/D-Gal-insulted mice. Pretreatment with compound C suppressed LPS/D-Gal-induced phosphorylation of JNK. Inhibition of JNK alleviated LPS/D-Gal-induced liver injury, but the level of p53 remained unchanged in mice exposed to LPS/D-Gal. Post-insult treatment with the AMPK activator A-769662 further increased the phosphorylation levels of AMPK and JNK, enhanced hepatocyte apoptosis and deteriorated liver injury, all of these effects could be reversed by co-administration of the AMPK inhibitor or JNK inhibitor. Interestingly, post-insult treatment with the AMPK inhibitor also resulted in beneficial outcomes. These data suggested that AMPK might be a late detrimental factor in LPS/D-Gal-induced hepatitis via potentiating JNK-dependent hepatocyte apoptosis and AMPK might become a pharmacological target for the intervention of fulminant hepatitis.
A novel mutation in the C-propeptide of COL2A1 causes atypical spondyloepiphyseal dysplasia congenita
Authors: Kusano Chieko , Takagi Masaki , Hori Naoaki , Murotsuki Jun , Nishimura Gen , Hasegawa Tomonobu 
Published: March 2017

AbstractSpondyloepiphyseal dysplasia congenita (SEDC, OMIM #183900) is one of the type II collagenopathies caused by a heterozygous mutation in the COL2A1 gene. Although typical SEDC shows delay of pubic bone ossification on radiographs, atypical SEDC exists without this finding. We identified an atypical SEDC patient with a novel missense mutation in the C-propeptide region of COL2A1. This case suggests that a COL2A1 C-propeptide mutation can cause atypical SEDC.
The respective effect of under-rib convection and pressure drop of flow fields on the performance of PEM fuel cells
Authors: Wang Chao , Zhang Qinglei , Shen Shuiyun , Yan Xiaohui , Zhu Fengjuan , Cheng Xiaojing , Zhang Junliang 
Published: March 2017

AbstractThe flow field configuration plays an important role on the performance of proton exchange membrane fuel cells (PEMFCs). For instance, channel/rib width and total channel cross-sectional area determine the under-rib convection and pressure drop respectively, both of which directly influence the water removal, in turn affecting the oxygen supply and cathodic oxygen reduction reaction. In this study, effects of under-rib convection and pressure drop on cell performance are investigated experimentally and numerically by adjusting the channel/rib width and channel cross-sectional area of flow fields. The results show that the performance differences with various flow field configurations mainly derive from the oxygen transport resistance which is determined by the water accumulation degree, and the cell performance would benefit from the narrower channels and smaller cross sections. It reveals that at low current densities when water starts to accumulate in GDL at under-rib regions, the under-rib convection plays a more important role in water removal than pressure drop does; in contrast, at high current densities when water starts to accumulate in channels, the pressure drop dominates the water removal to facilitate the oxygen transport to the catalyst layer.
Revealing the sequence of interactions of PuroA peptide with Candida albicans cells by live-cell imaging
Authors: Shagaghi Nadin , Bhave Mrinal , Palombo Enzo A. , Clayton Andrew H. A. 
Published: March 2017

AbstractTo determine the mechanism(s) of action of antimicrobial peptides (AMPs) it is desirable to provide details of their interaction kinetics with cellular, sub-cellular and molecular targets. The synthetic peptide, PuroA, displays potent antimicrobial activities which have been attributed to peptide-induced membrane destabilization, or intracellular mechanisms of action (DNA-binding) or both. We used time-lapse fluorescence microscopy and fluorescence lifetime imaging microscopy (FLIM) to directly monitor the localization and interaction kinetics of a FITC- PuroA peptide on single Candida albicans cells in real time. Our results reveal the sequence of events leading to cell death. Within 1 minute, FITC-PuroA was observed to interact with SYTO-labelled nucleic acids, resulting in a noticeable quenching in the fluorescence lifetime of the peptide label at the nucleus of yeast cells, and cell-cycle arrest. A propidium iodide (PI) influx assay confirmed that peptide translocation itself did not disrupt the cell membrane integrity; however, PI entry occurred 25–45 minutes later, which correlated with an increase in fractional fluorescence of pores and an overall loss of cell size. Our results clarify that membrane disruption appears to be the mechanism by which the C. albicans cells are killed and this occurs after FITC-PuroA translocation and binding to intracellular targets.
Rising nutrient-pulse frequency and high UVR strengthen microbial interactions
Authors: Cabrerizo Marco J. , Medina-Sánchez Juan Manuel , Dorado-García Irene , Villar-Argaiz Manuel , Carrillo Presentación 
Published: March 2017

AbstractSolar radiation and nutrient pulses regulate the ecosystem’s functioning. However, little is known about how a greater frequency of pulsed nutrients under high ultraviolet radiation (UVR) levels, as expected in the near future, could alter the responses and interaction between primary producers and decomposers. In this report, we demonstrate through a mesocosm study in lake La Caldera (Spain) that a repeated (press) compared to a one-time (pulse) schedule under UVR prompted higher increases in primary (PP) than in bacterial production (BP) coupled with a replacement of photoautotrophs by mixotrophic nanoflagellates (MNFs). The mechanism underlying these amplified phytoplanktonic responses was a dual control by MNFs on bacteria through the excretion of organic carbon and an increased top-down control by bacterivory. We also show across a 6-year whole-lake study that the changes from photoautotrophs to MNFs were related mainly to the frequency of pulsed nutrients (e.g. desert dust inputs). Our results underscore how an improved understanding of the interaction between chronic and stochastic environmental factors is critical for predicting ongoing changes in ecosystem functioning and its responses to climatically driven changes.
Evidence for history-dependence of influenza pandemic emergence
Authors: Hill Edward M. , Tildesley Michael J. , House Thomas 
Published: March 2017

AbstractInfluenza A viruses have caused a number of global pandemics, with considerable mortality in humans. Here, we analyse the time periods between influenza pandemics since 1700 under different assumptions to determine whether the emergence of new pandemic strains is a memoryless or history-dependent process. Bayesian model selection between exponential and gamma distributions for these time periods gives support to the hypothesis of history-dependence under eight out of nine sets of modelling assumptions. Using the fitted parameters to make predictions shows a high level of variability in the modelled number of pandemics from 2010–2110. The approach we take here relies on limited data, so is uncertain, but it provides cheap, safe and direct evidence relating to pandemic emergence, a field where indirect measurements are often made at great risk and cost.
Divergence of stable isotopes in tap water across China
Authors: Zhao Sihan , Hu Hongchang , Tian Fuqiang , Tie Qiang , Wang Lixin , Liu Yaling , Shi Chunxiang 
Published: March 2017

AbstractStable isotopes in water (e.g., δ2H and δ18O) are important indicators of hydrological and ecological patterns and processes. Tap water can reflect integrated features of regional hydrological processes and human activities. China is a large country with significant meteorological and geographical variations. This report presents the first national-scale survey of Stable Isotopes in Tap Water (SITW) across China. 780 tap water samples have been collected from 95 cities across China from December 2014 to December 2015. (1) Results yielded the Tap Water Line in China is δ2H = 7.72 δ18O + 6.57 (r2 = 0.95). (2) SITW spatial distribution presents typical “continental effect”. (3) SITW seasonal variations indicate clearly regional patterns but no trends at the national level. (4) SITW can be correlated in some parts with geographic or meteorological factors. This work presents the first SITW map in China, which sets up a benchmark for further stable isotopes research across China. This is a critical step toward monitoring and investigating water resources in climate-sensitive regions, so the human-hydrological system. These findings could be used in the future to establish water management strategies at a national or regional scale.