bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of genetic requirements and nutrient availability forStaphylococcus aureusgrowth in cystic fibrosis sputum
Authors: Shull Lauren M. , Wolter Daniel J. , Kunkle Dillon E. , Legg Katherine A. , Giedroc David P. , Skaar Eric P. , Hoffman Lucas R. , Reniere Michelle L. 
Published: September 2024

ABSTRACTStaphylococcus aureusis one of the most common pathogens isolated from the lungs of people with cystic fibrosis (CF), but little is known about its ability to colonize this niche. We performed a Tn-seq screen to identify genes necessary forS. aureusgrowth in media prepared fromex vivoCF sputum. We identified 19 genes that were required for growth in all sputum media tested and dozens more that were required for growth in at least one sputum medium. Depleted mutants of interest included insertions in many genes important for surviving metal starvation as well as the primary regulator of cysteine metabolismcymR. To investigate the mechanisms by which these genes contribute toS. aureusgrowth in sputum, we quantified low-molecular-weight thiols, nutrient transition metals, and the host metal-sequestration protein calprotectin in sputum from 11 individuals with CF. In all samples, the abundance of calprotectin exceeded nutrient metal concentration, explaining theS. aureusrequirement for metal-starvation genes. Further, all samples contain potentially toxic quantities of cysteine and sufficient glutathione to satisfy the organic sulfur requirements ofS. aureus. Deletion of the cysteine importer genestcyAandtcyPin the ΔcymRbackground restored growth to wild-type levels in CF sputum, suggesting that the mechanism by whichcymRis required for growth in sputum is to prevent uncontrolled import of cysteine or cystine from this environment. Overall, this work demonstrates that calprotectin and cysteine limitS. aureusgrowth in CF sputum.IMPORTANCEStaphylococcus aureusis a major cause of lung infections in people with cystic fibrosis (CF). This work identifies genes required forS. aureusgrowth in this niche, which represent potential targets for anti-Staphylococcal treatments. We show that genes involved in surviving metal starvation are required for growth in CF sputum. We also found that the primary regulator of cysteine metabolism, CymR, plays a critical role in preventing cysteine intoxication during growth in CF sputum. To support these models, we analyzed sputum from 11 individuals with CF to determine concentrations of calprotectin, nutrient metals, and low-molecular-weight thiols, which have not previously been quantified together in the same samples.
Staphylococcus aureusPhenol-Soluble Modulins Mediate Interspecies Competition with Upper Respiratory Commensal Bacteria
Authors: Huffines Joshua T , Kiedrowski Megan R 
Published: September 2024

ABSTRACTIn chronic rhinosinusitis (CRS) disease, microbial dysbiosis is considered a key contributor to inflammation and pathogenicity, with increased prevalence of upper respiratory tract (URT) pathogens concomitant with decreased abundance of commensal species.Staphylococcus aureusis a common URT pathobiont associated with higher carriage rates in CRS.S. aureussecreted toxins are implicated in CRS pathogenesis, and toxins and antibodies toS. aureussecreted factors have been observed in tissue from CRS subjects. CRS disease severity is positively correlated with immune reactivity toS. aureusproteins. Prior studies have examined polymicrobial interactions betweenS. aureusand URT commensals, however, no studies to date have described possible methods employed byS. aureusto outcompete commensals leading to aS. aureus-dominant microbiome as seen in CRS. This study addresses this gap in knowledge by characterizing how a CRS-associated secreted toxin fromS. aureuscan inhibit aggregation in commensal URT species. Using a model URT commensal,Corynebacterium pseudodiphtheriticum, we identified a CRS-associated secreted protein fromS. aureus, δ-toxin (Hld), that can inhibitC. pseudodiphtheriticumaggregation at biologically relevant concentrations. Furthermore, we observed recombinant δ-toxin reducesC. pseudodiphtheriticumadherence and aggregation on human nasal epithelial cells in an air-liquid interface cell culture model. These results define a novel mechanism by whichS. aureuscan disrupt URT commensal lifestyles of microbial competitors, contributing to the establishment of microbial dysbiosis.IMPORTANCEMicrobial dysbiosis in the upper respiratory tract (URT) is associated with disease pathogenicity in chronic rhinosinusitis (CRS). There are significant links betweenStaphylococcus aureusand worse CRS outcomes, but no studies to date have demonstrated ifS. aureusoutcompetes other URT microbes through direct interactions. Here, we report thatS. aureusδ-toxin, a secreted protein found in CRS patient tissue, can inhibit the ability of commensal bacteria to aggregate, adhere to, and grow in association with human nasal epithelial cells. These results suggest a potential mechanism forS. aureusto establish dominance in the URT microbiome through direct antagonism of commensals with a disease-associated toxin.
Elevated synaptic PKA activity and abnormal striatal dopamine signaling inAkap11mutant mice, a genetic model of schizophrenia and bipolar disorder
Authors: Song Bryan J. , Ge Yang , Nicolella Ally , Kwon Min Jee , Lodder Bart , Bonanno Kevin , Valle-Tojeiro Antia , Mandell Kira Perzel , Adeleye John , Geng Chuhan , Natarajan Sahana , Picard Inès , Shepard Nate , Aryal Sameer , Farsi Zohreh , Liu Xiao-Man , Morshed Nader , Nadaf Naeem M , Pribiag Horia , Simmons Sean K , Mani D.R. , Stevens Beth , Macosko Evan Z. , Levin Joshua Z. , Sabatini Bernardo L. , Keshishian Hasmik , Carr Steven A. , Dejanovic Borislav , Granger Adam J. , Sheng Morgan 
Published: September 2024

AbstractLoss-of-function mutations inAKAP11(a protein kinase A (PKA)-binding protein) greatly increase the risk of bipolar disorder and schizophrenia. To determine the neurobiological functions of AKAP11 and the consequences of its absence, we conducted multi-omic analyses ofAkap11mutant mouse brains. We find that AKAP11 is a key regulator of PKA proteostasis in the brain whose loss leads to dramatically elevated PKA expression and phosphorylation, especially in synapses. Transcriptomic analysis shows extensive gene expression changes throughout the brain, including prominent decreases in synapse-related genes sets. Gene expression is especially affected in spiny projection neurons of the striatum, a brain region implicated in motivation, cognition and psychiatric disorders.In vivo, real-time measurements of PKA activity in ventral striatum ofAkap11-/-mice revealed constitutively elevated kinase activity, which distorts dopamine to PKA signaling. Our work reveals the molecular basis of circuit dysfunction in a genetically valid model of psychotic disorder.
Intermediary business models: using blockchain technology for intermediary businesses
Authors: Purusottama Ambara , Trilaksono Teddy 
Published: September 2024

PurposeThis study aims to complement the literature disparity regarding the practice of technology in intermediary business models, which is still limited. The discussions of this study comprise (1) the comprehension of intermediary business models through building block modification and (2) the crystallization of blockchain adoption for intermediary business models.Design/methodology/approachThis study encourages the development of a new canvas through the iteration between theories and empirical evidence of intermediary business models, including using blockchains in this model. The new canvas was developed referring to the system complexity of the intermediate business model and confirmed using a single case study. The case studied was ALKO, which drives its business value by adopting blockchain technology. A few data sources were used to produce robust findings in this study.FindingsThe new canvas can elucidate the intermediary business model with designated case studies. Blockchain technology significantly contributes to the intermediary business model, where this technology can influence the entire activity system. The technology is being adopted as a “creation” for the firm to realize the “proposition” offered and “capture” value. In this typical business model, this technology is applied to implement shared values such as traceability, authenticity and integrity of information. This business model shows firm activities as coherent and cohesive relationships between blocks.Research limitations/implicationsBlockchain technology strengthens intermediary business models through its unique features. This study also describes the role of this technology in a particular system through the development of an intermediary business model canvas using a descriptive study. The intensity of this technology on a typical business model is clearly explained in this study.Originality/valueThis research brings a novel value in developing a canvas for intermediary business models and confirms the role of blockchain technology in this business model. The canvas design was carried out systematically, including explaining the contributions of blockchains in detail.
Does embeddedness in strategic alliances matter for innovation efficiency? The moderating roles of government R&D subsidies and standardization
Authors: Li Jian , Peng Di , Yu Yue 
Published: September 2024

PurposeThis paper investigates the impact of strategic alliance network centrality and structural holes on firm innovation efficiency. In addition, the paper aims to explore the moderating effects of government R&D subsidies and firm technology standardization.Design/methodology/approachBased upon the literature on strategic alliance networks, this paper proposes a conceptual model with several hypotheses. The empirical analysis is based on a sample of 736 observations from 92 mechanical manufacturing firms in China from 2010 to 2017. We measured firm innovation efficiency via the DEA model and performed quantitative analysis with GMM estimation.FindingsThe results indicate that strategic alliance network centrality is positively related to firm innovation efficiency, and structural holes have a U-shaped relationship with firm innovation efficiency. Government R&D subsidies positively moderate the relationship between centrality and firm innovation efficiency. Firm technology standardization positively moderates the relationship between centrality and firm innovation efficiency and the U-shaped relationship between structural holes and firm innovation efficiency.Practical implicationsFirms should focus on improving innovation efficiency and maximizing innovation output under limited resources. Furthermore, managers ought to strengthen cooperation between firms and external alliances while promoting the utilization of strategic alliance network position resources to benefit innovation efficiency.Originality/valueThis paper considers that innovation efficiency, including input and output processes, is more representative than innovation performance, and few studies have focused on the relationship between strategic alliance networks and innovation efficiency. To fill this research gap, this paper explores the impact of strategic alliance network position embedding on innovation efficiency.
Learning-based framework for industrial accident prevention: fuzzy cognitive mapping approach
Authors: Boulagouas Wafa , Guelfen Charaf Eddine , Karoune Abderraouf 
Published: September 2024

PurposeDespite efforts to improve safety management practices in industrial companies, major accidents seem to be inevitable. Many accidents still occur because companies are unable to learn from past occurrences due to ineffective incident and accident learning processes. This study proposes a learning-based framework for industrial accidents investigation and contributes to accident prevention research.Design/methodology/approachThe proposed learning process includes the analysis of the industrial accident using the Event Tree Analysis (ETA) method, capitalisation of causative factors using the Swiss Cheese Model (SCM), and finally modelling the relationships among the accident causative factors and analysing their causality using the Fuzzy Cognitive Mapping (FCM) technique and running learning scenarios.FindingsThe proposed learning process was applied to an industrial accident, and the results showed that human unsafe behaviours and unsafe supervision were the principal causative factors of the blowout accident.Practical implicationsThe proposed learning-based framework provides a structured approach for oil and gas companies to systematically analyse and learn from past accidents, enhancing their prevention strategies. Theoretically, the framework bridges the gap between theory and practice by demonstrating how established accident analysis methods can be combined and applied in a real-world industrial context.Originality/valueThe proposed learning process combines accident analysis and investigation techniques with simulations for an in-depth and robust learning-based framework for accident prevention.
Unraveling the impact of infodemic stress on information and health behaviors: a double effect perspective
Authors: Pan Ting , Sun Yongqiang , Guo Xitong , Zhang Min 
Published: September 2024

PurposeThis paper aims to explore the underlying mechanism whereby information-induced stress, resulting from the burdensome nature, questionable information reliability, misleading content and diffuse characteristics of infodemic pressure, impacts individuals’ online information-related behavior and health-related preventive behavior.Design/methodology/approachWe conducted a cross-sectional survey organized with social media users during the post-pandemic period. Based on the 342 valid responses, structural equation modeling was employed to validate the research model.FindingsThe results substantiate our multidimensional view of infodemic stress, which encompasses dimensions including information overload, uncertainty, diffusivity and insecurity. We found that the infodemic stress contributes to pandemic fatigue, then engenders both negative information behavior and reduced engagement in preventive behavior. Furthermore, infodemic stress has also been found to have a direct positive effect on individuals’ engagement in preventive behavior.Originality/valueThis study introduces the concept of “infodemic stress” and presents a comprehensive framework to capture its various dimensions. This study offers novel insights into the psychological and behavioral repercussions of the infodemic stress transmitted through social media.
Maida Herman Solomon (1891–1988): an invisible forerunner in the field of clinical social work
Authors: Williams Kristin S. 
Published: September 2024

Purpose
This paper aims (1) to create a sense of resonance with Maida Herman Solomon and her ideas, (2) to inspire a reconsideration of current management history (the unquestioned block box of dominant figures, dominant foci and dominant practices), (3) to bring Solomon’s contributions to clinical social work into present discourse in management and organizational studies and (4) to foster recognition for Solomon in her own field of social work, as forerunner in a developing profession. Guiding this study is the question: “What are Solomon’s key contributions and why is she overlooked?”

Design/methodology/approach
This paper features a novel methodology, ficto-feminism. The feminism in ficto-feminism is presented as ontology, epistemology, method and mode of writing. Ficto-feminism combines polemical (or prowoman writing) with aspects of collective biography, autoethnography and fictocriticism. As such, the paper contributes to the emerging feminist tradition of writing differently. The approach is an embodied and reflexive approach that engages with history to investigate the absence of women.

Findings
Maida Solomon was an educator, researcher, practitioner and advocate. Her contributions to the development and practice of clinical social work spanned over 60 years, and yet, she is little more than a footnote in the history of the field. Her contributions include authoring and implementing graduate programming, which continues to be the taken for granted training; penning some of the most seminal works and advancing theory; introducing academic and scientific approaches, which saw the field professionalize and adopt new standards; and helping to change the way that society thought about mental health and sexual health. A confluence of factors contributes to her marginalization and neglect: gender, ethnicity, the feminized field of social work and the stigmatized focus for her practice.

Originality/value
The paper combines assertive autobiographical and literary strategies to foreground an overlooked female leader in the field of clinical social work, namely, Maida Solomon. Drawing on biographical material, literature, media and archival material, this paper features a fictional but truthful conversation between the present-day author/writer/historian and the posthumous, historical protagonist (Maida Solomon). In so doing, the engagement with history is both one that deconstructs while reconstructing a historical account with both aesthetic and political implications.
Technological work burnout: conceptualization, measure development and validation
Authors: Alhammadi Hassan , Bani-Melhem Shaker , Mohd-Shamsudin Faridahwati , Karrani Mariam , Hamouche Salima 
Published: September 2024

PurposeAs workplaces increasingly integrate digital technologies, understanding their impact on employee burnout has become imperative. This paper introduces the Technological Work Burnout Scale (TWBS), an innovative tool developed to measure the influence of technology on workplace burnout.Design/methodology/approachTo bridge the existing gap in this area of study, our research employed Hinkin’s (1998) psychometric methodologies, creating a structured process for developing the TWBS, which included initial item generation, item reduction with reliability estimation, confirmatory factor analysis and tests for both convergent and discriminant validity. By applying these procedures, we validated the scale across various professional settings (in three different samples), ensuring its robustness and applicability in diverse technological work environments.FindingsThe TWBS demonstrates a consistent unidimensional structure, effectively capturing the multifaceted nature of burnout in the digital age.Originality/valueThrough this scale, we provide insights into how technology influences employee well-being and organizational health, offering a valuable tool for organizations to assess and manage the growing issue of technological work burnout. Our study not only enriches the academic understanding of burnout in the context of technological integration in workplaces but also offers practical implications for addressing this critical concern.
Comprehensive sustainability assessment of Ferrock: innovations for the sustainable built environment
Authors: Muhit Imrose B. , Al-Fakih Amin , Mbiu Ronald Ndung’u 
Published: September 2024

PurposeThis study aims to evaluate the suitability of Ferrock as a green construction material by analysing its engineering properties, environmental impact, economic viability and adoption challenges. It also aims to bridge knowledge gaps and provide guidance for integrating Ferrock into mainstream construction to support the decarbonisation of the built environment.Design/methodology/approachIt presents a systematic and holistic review of existing literature on Ferrock, comprehensively analysing its mechanical properties, environmental and socio-economic impact and adoption challenges. The approach includes evaluating both quantitative and qualitative data to assess Ferrock’s potential in the construction sector.FindingsKey findings highlight Ferrock’s superior mechanical properties, such as higher compressive and tensile strength, and enhanced durability compared to traditional Portland cement. Ferrock offers significant environmental benefits by capturing more CO2 during curing than it emits, contributing to carbon sequestration and reducing energy consumption due to the absence of high-temperature processing. However, the material faces economic and technical challenges, including higher initial costs, scalability issues, lack of industry standards and variability in production quality.Originality/valueThis review provides a comprehensive and up-to-date analysis of Ferrock. Despite being discussed for a decade, Ferrock research has been overlooked, with existing studies often limited and published in poor-quality sources. By synthesising current research and identifying future study areas, the paper enhances understanding of Ferrock’s potential benefits and challenges. The originality lies in the holistic evaluation of Ferrock’s properties and its implications for the construction industry, offering insights that could drive collaborative research and policy support to facilitate its integration into mainstream use.
Influential factors in general public opposition and public servant concerns toward municipal DX promotion in Japan
Authors: Seo Yuna 
Published: September 2024

Purpose
This study aims to investigate the factors influencing public servants’ anxiety and general public’s opposition toward the implementation of digital participatory platforms (DPPs) and municipal digital transformation (DX) in Japan. By addressing these factors, the research seeks to provide insights for policymakers to facilitate smoother transitions to digital governance and increase public acceptance and engagement.

Design/methodology/approach
The study uses surveys conducted with both general public and public servants. The surveys were designed based on previous research and collected data through Web-based questionnaires. General public’s data were collected from 366 valid responses over four days in July 2022, while public servants’ data were gathered from 197 valid responses over eight days. Statistical analysis was used to identify key factors influencing anxiety and opposition.

Findings
Public servants’ anxiety is influenced by self-perceived creativity, openness to innovation, international collaborations, work-life balance and gender equality reforms. The general public’s opposition stems from dissatisfaction with current digital tools, social media use and political engagement. Both groups could benefit from targeted training, improved usability and inclusive engagement strategies.

Practical implications
The findings suggest that targeted training to enhance public servants’ creativity and digital literacy, fostering an innovative organizational culture and promoting work-life balance can reduce anxiety about DPPs. For general public, improving the usability of digital tools, engaging them in the design process and leveraging social media for communication and feedback can increase acceptance of digital initiatives.

Social implications
Understanding the concerns of both public servants and general public regarding DX can lead to more inclusive and effective governance. By addressing these concerns, policymakers can foster greater public trust and engagement, ultimately enhancing the effectiveness and transparency of municipal governance.

Originality/value
This study provides a comprehensive analysis of the factors contributing to resistance to DX in public governance. By examining both public servants’ and general public’ perspectives, it offers valuable insights for designing and implementing strategies to facilitate smoother transitions to digital participatory governance.