bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional specialization of intestinal dendritic cell subsets during Th2 helminth infection in mice
Authors: Redpath Stephen A. , Heieis Graham A. , Reynolds Lisa A. , Fonseca Nicolette M. , Kim Sandra S.‐Y. , Perona‐Wright Georgia 
Published: September 2017

AbstractDendritic cells (DCs) are essential in dictating the nature and effectiveness of immune responses. In the intestine DCs can be separated into discrete subsets, defined by expression of CD11b and CD103, each with different developmental requirements and distinct functional potential. Recent evidence has shown that different intestinal DC subsets are involved in the induction of T helper (Th)17 and regulatory T cell responses, but the cells that initiate Th2 immune responses are still incompletely understood. We show that in the Th2 response to an intestinal helminth in mice, only CD11b+ and not CD11b− DCs accumulate in the local lymph node, upregulate PDL2 and express markers of alternative activation. An enteric Th1 response instead activated both CD11b+ and CD11b− DCs without eliciting alternative activation in either population. Functionally, only CD11b+ DCs activated during helminth infection supported Th2 differentiation in naive CD4+ T cells. Together our data demonstrate that the ability to prime Th2 cells during intestinal helminth infection, is a selective and inducible characteristic of CD11b+ DCs.
Network information and cross‐border M&A activities
Authors: Xia Jun , Ma Xufei , Tong Tony W. , Li Weiwen 
Published: September 2017

Research SummaryHow information about social networks affects firms’ global expansion is an important but rarely investigated question. We argue that different sources of network information will affect a firm's investment activities in the global market, but their relative importance may vary. Moreover, the influence of network information can be bounded by the global trade network. The results from a sample of cross‐border merger and acquisition (M&A) activities conducted by U.S. publicly listed firms reveal that although M&A experience of both interlocking and joint venture partners affects the focal firm's global expansion, the effect of interlocking partners is more influential than joint venture partners. Meanwhile, when mutual trade dependence between countries is high, the positive effect of network information from board interlocks diminishes.Managerial SummaryFirms are hesitant to engage in cross‐border M&As, because it is difficult for them to get information about local business practices and environmental idiosyncrasies. This study shows that firms can deal with this challenge by accessing relevant information from the M&A experience of interlocking partners and joint venture partners. The information generated from the M&A experience of interlocking partners is even more valuable and important than that of joint venture partners in shaping firms’ cross‐border M&A decisions. Moreover, the M&A experience of interlocking partners and joint venture partners is particularly valuable for firms contemplating M&A activities in a country with relatively fewer buyer‐seller exchange networks with their home country.
Hypermethylated ZNF582 and PAX1 genes in mouth rinse samples as biomarkers for oral dysplasia and oral cancer detection
Authors: Cheng Shih‐Jung , Chang Chi‐Feng , Ko Hui‐Hsin , Lee Jang‐Jaer , Chen Hsin‐Ming , Wang Huei‐Jen , Lin Hsiao‐Shan , Chiang Chun‐Pin 
Published: September 2017

AbstractBackgroundEffective biomarkers for oral cancer screening are important for early diagnosis and treatment of oral cancer.MethodsOral epithelial cell samples collected by mouth rinse were obtained from 65 normal control subjects, 108 patients with oral potentially malignant disorders, and 94 patients with oral squamous cell carcinoma (OSCC). Methylation levels of zinc‐finger protein 582 (ZNF582) and paired‐box 1 (PAX1) genes were quantified by real‐time methylation‐specific polymerase chain reaction after bisulfite conversion.ResultsAn abrupt increase in methylated ZNF582 (ZNF582m) and PAX1 (PAX1m) levels and positive rates from mild dysplasia to moderate/severe dysplasia, indicating that both ZNF582m and PAX1m are effective biomarkers for differentiating moderate dysplasia or worse (MODY+) oral lesions. When ZNF582m/PAX1m tests were used for identifying MODY+ oral lesions, the sensitivity, specificity, and odds ratio (OR) were 0.65/0.64, 0.75/0.82, and 5.6/8.0, respectively.ConclusionHypermethylated ZNF582 and PAX1 genes in oral epithelial cells collected by mouth rinse are effective biomarkers for the detection of oral dysplasia and oral cancer.
Cross‐sectional study of the case–fatality rate among patients with visceral leishmaniasis infections during pregnancy in Sudan
Authors: Adam Gamal K. , Omar Saeed M. , Ahmed MohammedAhmed A. , Abdallah Tajeldin M. , Ali AbdelAziem A. 
Published: September 2017

Among patients who were pregnant and had visceral leishmaniasis infections at Gadarif Hospital, Sudan, a case–fatality rate of 18% was recorded.
Aberrant expression of miR‐451a contributes to 1,2‐dichloroethane‐induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice
Authors: Zeng Ni , Jiang Hongmei , Fan Qiming , Wang Ting , Rong Weifeng , Li Guoliang , Li Ruobi , Xu Dandan , Guo Tao , Wang Fei , Zeng Lihai , Huang Manqi , Zheng Jiewei , Lu Fengrong , Chen Wen , Hu Qiansheng , Huang Zhenlie , Wang Qing 
Published: September 2017

AbstractThe identification of aberrant microRNA (miRNA) expression during chemical‐induced hepatic dysfunction will lead to a better understanding of the substantial role of miRNAs in liver diseases. 1,2‐Dichloroethane (1,2‐DCE), a chlorinated organic toxicant, can lead to hepatic abnormalities in occupationally exposed populations. To explore whether aberrant miRNA expression is involved in liver abnormalities mediated by 1,2‐DCE exposure, we examined alterations in miRNA expression patterns in the livers of NIH Swiss mice after dynamic inhalation exposure to 350 or 700 mg m–3 1,2‐DCE for 28 days. Using a microarray chip, we discovered that only mmumiR‐451a was significantly upregulated in the liver tissue of mice exposed to 700 mg m–3 1,2‐DCE; this finding was validated by quantitative real‐time polymerase chain reaction. In vitro study revealed that it was metabolite 2‐chloroacetic acid, not 1,2‐DCE that resulted in the upregulation of mmu‐miR‐451a in the mouse AML12 cell line. Furthermore, our data showed that the upregulation of mmu‐miR‐451a induced by 2‐chloroacetic acid could suppress the expression of glycerol kinase and lead to the inhibition of glycerol gluconeogenesis in mouse liver tissue and AML12 cells. These observations provide evidence that hepatic mmu‐miR‐451a responds to 1,2‐DCE exposure and might induce glucose metabolism disorders by suppressing the glycerol gluconeogenesis process.
Diethylstilbestrol impaired oogenesis of yellow catfish juveniles through disrupting hypothalamic–pituitary–gonadal axis and germ cell development
Authors: Wang Yaqin , Li Yingwen , Chen Qiliang , Liu Zhihao 
Published: September 2017

AbstractDiethylstilbestrol (DES), a non‐steroidal estrogen, has been found to cause altered germ cell development and disordered ovarian development in fish females. However, the mechanisms that might be involved are poorly understood. In this study, female juveniles of yellow catfish (Pelteobagrus fulvidraco) (120 days post‐hatching) were exposed to two doses (10 and 100 ng l−1) of DES for 28 days. After the endpoint of exposure, decreased ovary weight and gonadosomatic index, as well as various ovarian impairments were observed in response to DES. Besides, DES elevated the mRNA levels of vitellogenin 1 (vtg 1) and estrogen receptor 1 (esr 1) in liver and decreased 17β‐estradiol level in plasma. Correspondingly, suppressed mRNA levels of the key genes in the hypothalamic–pituitary–gonadal axis (such as cyp19a1b, gnrh‐II, fshβ and lhβ in brain and fshr, lhr and cyp19a1a in ovary) after DES exposure were also observed. The declined level of plasma 17β‐estradiol and altered gene expressions of genes in the hypothalamic–pituitary–gonadal axis were thus supposed to be closely related to the disrupted oogenesis in DES‐treated fish. Analyses further demonstrated that, higher concentration of DES elevated the expression ratio of bax/bcl‐2, indicating the enhanced apoptosis occurred in ovary. Moreover, DES upregulated the expressions of genes involved in proliferation (cyclin d1 and pcna), meiotic entry (cyp26a1 and scp3) and meiotic maintenance (dmc1), resulting in arrested oogenesis in catfish. The present study greatly extended our understanding on the mechanisms underlying of reproductive toxicity of DES on fish oogenesis.
Evaluation of Radius Microstructure and Areal Bone Mineral Density Improves Fracture Prediction in Postmenopausal Women
Authors: Biver Emmanuel , Durosier-Izart Claire , Chevalley Thierry , van Rietbergen Bert , Rizzoli René , Ferrari Serge 
Published: September 2017

ABSTRACT

A majority of low-trauma fractures occur in subjects with only moderate decrease of areal bone mineral density (aBMD), ie, osteopenia, assessed by dual-energy X-ray absorptiometry (DXA) or low fracture probability assessed by FRAX. We investigated whether peripheral bone microstructure and estimated strength improve the prediction of incident fractures beyond central DXA and FRAX. In this population-based study of 740 postmenopausal women (aged 65.0 ± 1.4 years) from the Geneva Retirees Cohort (ISRCTN registry 11865958), we assessed at baseline cortical (Ct) and trabecular (Tb) volumetric bone mineral density (vBMD) and microstructure by peripheral quantitative computed tomography (HR-pQCT); bone strength by micro-finite element analysis; aBMD and trabecular bone score (TBS) by DXA; and FRAX fracture probability. Eighty-five low-trauma fractures occurred in 68 women over a follow-up of 5.0 ± 1.8 years. Tb and Ct vBMD and microstructure predicted incident fractures, independently of each other and of femoral neck (FN) aBMD and FRAX (with BMD ± TBS). However, the associations were markedly attenuated after adjustment for ultra-distal radius aBMD (same bone site). The best discrimination between women with and without fracture was obtained at the radius with total vBMD, the combination of a Tb with a Ct parameter, or with failure load, which improved the area under the curve (AUC) for major osteoporotic fracture when added to FN aBMD (0.760 versus 0.695, p = 0.022) or to FRAX-BMD (0.759 versus 0.714, p = 0.015). The replacement of failure load by ultra-distal aBMD did not significantly decrease the AUC (0.753, p = 0.747 and 0.750, p = 0.509, respectively). In conclusion, peripheral bone microstructure and strength improve the prediction of fractures beyond central DXA and FRAX but are partially captured in aBMD measured by DXA at the radius. Because HR-pQCT is not widely available for clinical purposes, assessment of ultra-distal radius aBMD by DXA may meanwhile improve fracture risk estimation. © 2017 American Society for Bone and Mineral Research.
Antioxidant activities of novel resveratrol analogs in breast cancer
Authors: Chatterjee Anwesha , Ronghe Amruta , Padhye Subhash B. , Spade David A. , Bhat Nimee K. , Bhat Hari K. 
Published: September 2017

AbstractThe objective of the present study was to characterize the role of novel resveratrol (Res) analogs: 4‐(E)‐{(4‐hydroxyphenylimino)‐methylbenzene, 1, 2‐diol} (HPIMBD) and 4‐(E)‐{(p‐tolylimino)‐methylbenzene‐1,2‐diol} (TIMBD) as potent antioxidants against breast cancer. Non‐neoplastic breast epithelial cell lines MCF‐10A and MCF‐10F were treated with 17β‐estradiol (E2), Res, HPIMBD, and TIMBD for up to 72 h. mRNA and protein levels of antioxidant genes, superoxide dismutase 3 (SOD3) and N‐quinoneoxidoreductase‐1 (NQO1) and transcription factors, nuclear factor erythroid 2‐related factor (Nrf) 1, 2 and 3 were quantified after the above treatments. Generation of reactive oxygen species (ROS) was measured by CM‐H2‐DCFDA and oxidative‐DNA damage was determined by measuring 8‐hydroxy‐2‐deoxyguanosine (8‐OHdG). HPIMBD and TIMBD scavenged cellular ROS production, attenuated oxidative DNA damage, increased mRNA and protein expression levels of SOD3 and NQO1 and activated Nrf signaling pathway. Our studies demonstrate that HPIMBD and TIMBD have the potential as novel antioxidants to prevent development of breast cancer.
Triggering p53 activation is essential in ziyuglycoside I‐induced human retinoblastoma WERI‐Rb‐1 cell apoptosis
Authors: Zhu Xue , Wang Ke , Yao Yong , Zhang Kai , Zhou Fanfan , Zhu Ling 
Published: September 2017

AbstractZiyuglycoside I (Ziyu I), one of the major components isolated from the root of Sanguisorba officinalis L., has been proved for the antitumor properties on oral cancer, prostate cancer, and colorectal cancer. However, the effect of Ziyu I on retinoblastoma (RB) is not well understood. In this study, we investigated the inhibitory effect and underlying molecular mechanism of Ziyu I on human RB WERI‐Rb‐1 cells. Our results indicated that Ziyu I could suppress cell viability and induce mitochondrial‐dependent cell apoptosis in WERI‐Rb‐1 cells. Furthermore, Ziyu I treatment increased p53 expression as well as improved p53 stabilization through downregulation of pS166‐Mdm2 and upregulation of phosphorylated‐ and acetylated‐p53. Blockade of p53 significantly attenuated Ziyu I‐induced mitochondrial dysfunction. Our findings demonstrate that Ziyu I exhibits excellent anticancer effect on human RB WERI‐Rb‐1 cells by triggering p53 activation, and imply Ziyu I as a potential compound for chemotherapy of human RB.
Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption
Authors: Dou Yi‐Fang , Zhang Dong‐Hao , Liu Hang , Xia Yu‐Pei , Zhi Gao‐Ying 
Published: September 2017

AbstractBACKGROUNDPolyethyleneimine (PEI) has attracted wide interest due to not only its good hydrophilicity but also its high density of amino groups across the polymer chain. In order to study in depth the effect of PEI as spacer arm on dye ligand modification and further protein adsorption, poly(methylmethacrylate) (PMMA) magnetic microspheres were fabricated, grafted with PEI, and further modified with an affinity dye‐ligand, Cibacron Blue F3GA (CB).RESULTSCB density of these CB‐PEI‐PMMA microspheres was determined to be 370.7 µmol g‐1 for CB‐PEI 600‐PMMA, 355.2 µmol g‐1 for CB‐PEI 1800‐PMMA, and 317.6 µmol g‐1 for CB‐PEI 70000‐PMMA microspheres, respectively. Moreover, bovine serum albumin (BSA) adsorption capacity of PEI‐PMMA and CB‐PEI‐PMMA microspheres increased greatly compared with PMMA microspheres. The effects of temperature, BSA initial concentration, pH and ionic strength on BSA adsorption were studied. CB‐PEI 70000‐PMMA microspheres exhibited the maximum adsorption of 103.6 mg g‐1.CONCLUSIONThe results demonstrated the versatility of PEI as spacer arm. The information obtained from the present work could be utilized for rational design of carriers for protein adsorption or enzyme immobilization. © 2017 Society of Chemical Industry
Pilot scale production of extracellular thermo‐alkali stable laccase from Pseudomonas sp. S2 using agro waste and its application in organophosphorous pesticides degradation
Authors: Chauhan Prakram Singh , Jha Bhavanath 
Published: September 2017

AbstractBackgroundLaccases are multicopper oxidases that are able to oxidize various aromatic or nonaromatic compounds owing to their multifarious applications. However, till now only a few bacterial laccases have been isolated and characterized. Hence there is an urgent need to study an extracellular thermo‐alkali stable laccase.ResultsIn the present study, an extracellular thermo‐alkali stable laccase was produced from Pseudomonas sp. S2 in a 100 L bioreactor using agro waste (potato peel). Production was 17‐fold higher than in the control. The enzyme (S2LAC) was purified 12.16 ± 1.6‐fold to homogeneity with specific activity of 1089.70 ± 16.8 U mg−1 and molecular mass of 38 kDa. The temperature and pH for maximum enzyme activity were 80 °C and 9.0, respectively. The metal ions Na+, K+, Pb+2, Ca+2, Cu+2 and Co+2 enhanced enzyme activity. The purified enzyme showed maximum specificity to Pyrogallol > PPD > L‐DOPA > Hydroquinone. The S2LAC was able to degrade organ‐phosphorous pesticide including dichlorophos, chlorpyrifos, monocrotophos and profenovos upto 45.99 ± 0.3%, 80.56 ± 0.6%, 75.45 ± 1.3%, 81.84 ± 0.6%, respectively, in the absence of any mediator.ConclusionS2LAC produced using agro waste was stable and capable of degrading organophosphorous pesticides making it attractive for industrial applications. © 2017 Society of Chemical Industry
Developing risk management dashboards using risk and quality measures: A visual best practices approach
Authors: Bunting Robert F. , Siegal Dana 
Published: September 2017

AbstractBecause quality measures are ubiquitous, health care risk management leaders often use them as a proxy for risk management measures. While certain quality measures adequately reflect some aspects of risk management, they are neither a perfect nor complete substitute for well‐developed and comprehensive risk management measures. Using a comprehensive approach consisting of quality measures, risk measures, and measures that are less amenable to classification would be the best approach. Identifying the most powerful and informative measures, designing the most appropriate dashboards, and incorporating visual best practices are crucial steps required for evaluating the effectiveness and value of an enterprise risk management program. The authors explain the terms and concepts, review the measures available in the literature, propose new measures, discuss visual best practices, and provide sample dashboard components.
Robust motion correction for myocardial T1 and extracellular volume mapping by principle component analysis‐based groupwise image registration
Authors: Tao Qian , van der Tol Pieternel , Berendsen Floris F. , Paiman Elisabeth H.M. , Lamb Hildo J. , van der Geest Rob J. 
Published: September 2017

BackgroundMyocardial tissue characterization by MR T1 and extracellular volume (ECV) mapping has demonstrated clinical value. The modified Look–Locker inversion recovery (MOLLI) sequence is a standard mapping technique, but its quality can be negatively affected by motion.PurposeTo develop a robust motion correction method for T1 and ECV mapping.Study TypeRetrospective analysis of clinical data.PopulationFifty patients who were referred to cardiac MR exam for T1 mapping.Field Strength/Sequence3.0T cardiac MRI with precontrast and postcontrast MOLLI acquisition of the left ventricle (LV).AssessmentA groupwise registration method based on principle component analysis (PCA) was developed to register all MOLLI frames simultaneously. The resulting T1 and ECV maps were compared to those from the original and motion‐corrected MOLLI with pairwise registration, in terms of standard deviation (SD) error.Statistical TestPaired variables were compared using the Wilcoxon signed‐rank test.ResultsThe groupwise registration method demonstrated improved registration performance compared to pairwise registration, with the T1 SD error reduced from 31 ± 20 msec to 26 ± 15 msec (P < 0.05), and ECV SD error reduced from 4.1 ± 3.6% to 2.8 ± 2.0% (P < 0.05). In LV segmental analysis, the performance was particularly improved in lateral segments, which are most affected by motion. The running time of groupwise registration was significantly shorter than that of the pairwise registration, 17.5 ± 3.0 seconds compared to 43.5 ± 2.2 seconds (P < 0.05).Data ConclusionWe developed an automatic, robust motion correction method for myocardial T1 and ECV mapping based on a new groupwise registration scheme. The method led to lower mapping error compared to the conventional pairwise registration method in reduced execution time.Level of Evidence: 3Technical Efficacy: Stage 1J. Magn. Reson. Imaging 2018;47:1397–1405.
Quantitation of anthocyanins in elderberry fruit extracts and nutraceutical formulations with paper spray ionization mass spectrometry
Authors: Cody Robert B. , Tamura Jun , Downard Kevin M. 
Published: September 2017

AbstractThe ability to rapidly identify and quantitate, over a wide range of concentrations, anthocyanins in food and therapeutic products is important to ensuring their presence at medicinally significant levels. Sensitive, yet mild, analysis conditions are required given their susceptibility to degradation and transformation. Paper spray ionization has been used to detect and quantify the levels of anthocyanin levels in extracts of fresh and dried elderberries, and elderberry stems, as well as 3 commercially available nutraceutical formulations. The component cyanidin glucosides, including cyanidin‐3‐sambubioside, cyanidin‐3‐glucoside, cyanidin‐3,5‐diglucoside, cyanidin‐3‐sambubioside‐5‐glucoside, and the aglycone cyanidin, were readily detected in a range of sources. Quantitation was achieved by establishing a calibration plot from dilutions of a stock solution of cyanidin‐3,5‐diglucoside containing malvidin‐3,5‐diglucoside as an internal standard at a fixed concentration. The same standard was used to quantify the anthocyanin content in the fruit and nutraceutical formulations. Wide 5‐fold variations in anthocyanin concentration were detected in the nutraceutical formulations from different suppliers ranging from 1050 to 5430 mg/100 g. These concentrations compared with 500 to 2370 mg/100 g measured in the dried stems and fruit, respectively.
5′‐methylschweinfurthin G reduces chondrosarcoma tumor growth
Authors: Stevens Jeff W. , Meyerholz David K. , Neighbors Jeffery D. , Morcuende José A. 
Published: September 2017

ABSTRACTNew treatment options are urgently required in the field of chondrosarcoma, particularly of chondrosarcomas with a well‐differentiated hyaline cartilage‐like extracellular matrix (e.g., collagen II and proteoglycan‐rich) phenotype, notoriously resistant to drug penetration, and having potential of progression towards higher grade. We investigated the feasibility of using 5′‐methylschweinfurthin G (MeSG) as a tumor suppressor agent in the Swarm rat chondrosarcoma, an intermediate‐ to high‐grade chondrosarcoma model, having a hyaline cartilage‐like phenotype. Tumor cell culture studies were performed to identify their proliferative and cytotoxicity sensitivity to MeSG. Tumor burden mice were treated with MeSG and analyzed for tumor growth, morphology and regression. The chondrosarcoma tumor cells had a half maximum cytotoxicity concentration (IC50) of 35 nM MeSG; approximately 300‐fold less than freshly isolated rat chondrocytes (IC50of 11 µM). Multiple injections of MeSG (20 mg/kg, body weight) resulted in reduced/eliminated tumor growth over a 17‐day period in mice, and an 83% reduction (p = 0.023) in tumor mass. Three out of ten MeSG treated mice had complete elimination of tumor. Tumors of treated mice had a decrease in chondrosarcoma cell proliferation (p = 0.012) and an increase in cell death (p = 0.030) compared with tumors of control mice. These findings in an animal model demonstrate the effectiveness of MeSG for treatment of rat chondrosarcomas, and may have the potential use as a therapeutic option for the difficult‐to‐treat intermediate‐to high‐grade hyaline cartilage‐like chondrosarcoma. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1283–1293, 2018.