bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Vimentin Expression Associated with Lymph Node Metastasis and Predicated a Poor Prognosis in Oral Squamous Cell Carcinoma
Authors: Liu Shuli , Liu Liu , Ye Weimin , Ye Dongxia , Wang Tong , Guo Wenzheng , Liao Yueling , Xu Dongliang , Song Hongyong , Zhang Ling , Zhu Hanguang , Deng Jiong , Zhang Zhiyuan 
Published: December 2016

AbstractOral squamous cell carcinoma (OSCC) is a common public health problem worldwide with poor prognosis, which is largely due to lymph node metastasis and recurrence. Identification of specific molecular markers of OSCC with lymph node metastasis would be very important for early and specific diagnosis. In this study, we screened for the potential prognosis markers via unbiased transcriptomic microarray analysis in paired two OSCC cell lines, a lymph node metastatic HN12 cell line and a low metastatic parental HN4 cell line. The results showed that vimentin, with 87-fold increase of expression, was on the top of all upregulated genes in metastatic HN12 cells compared to non-metastatic HN4 cells. Treatment of non-metastatic HN4 cells with TGF-β1 induced epithelial to mesenchymal transition (EMT), with increased vimentin expression as well as enhanced migration activity. Consistently, knockdown of vimentin via siRNA resulted in suppressed invasion and migration activities of HN12 cells, suggesting an essential role of vimentin in EMT-related functions of OSCC cells. Finally, immunohistochemical (IHC) staining analysis showed that high vimentin expression was strongly associated with high lymph node metastases (p < 0.05), and poor overall survival (p < 0.05) in OSCC patients. Thus, high vimentin expression is strongly associated with increased metastatic potential, and may serve as a prediction marker for poor prognosis in OSCC patients.
Sympathetic neurons are a powerful driver of myocyte function in cardiovascular disease
Authors: Larsen Hege E. , Lefkimmiatis Konstantinos , Paterson David J. 
Published: December 2016

AbstractMany therapeutic interventions in disease states of heightened cardiac sympathetic activity are targeted to the myocytes. However, emerging clinical data highlights a dominant role in disease progression by the neurons themselves. Here we describe a novel experimental model of the peripheral neuro-cardiac axis to study the neuron’s ability to drive a myocyte cAMP phenotype. We employed a co-culture of neonatal ventricular myocytes and sympathetic stellate neurons from normal (WKY) and pro-hypertensive (SHR) rats that are sympathetically hyper-responsive and measured nicotine evoked cAMP responses in the myocytes using a fourth generation FRET cAMP sensor. We demonstrated the dominant role of neurons in driving the myocyte ß-adrenergic phenotype, where SHR cultures elicited heightened myocyte cAMP responses during neural activation. Moreover, cross-culturing healthy neurons onto diseased myocytes rescued the diseased cAMP response of the myocyte. Conversely, healthy myocytes developed a diseased cAMP response if diseased neurons were introduced. Our results provide evidence for a dominant role played by the neuron in driving the adrenergic phenotype seen in cardiovascular disease. We also highlight the potential of using healthy neurons to turn down the gain of neurotransmission, akin to a smart pre-synaptic ß-blocker.
Bell’s Nonlocality Can be Detected by the Violation of Einstein-Podolsky-Rosen Steering Inequality
Authors: Chen Jing-Ling , Ren Changliang , Chen Changbo , Ye Xiang-Jun , Pati Arun Kumar 
Published: December 2016

AbstractRecently quantum nonlocality has been classified into three distinct types: quantum entanglement, Einstein-Podolsky-Rosen steering, and Bell’s nonlocality. Among which, Bell’s nonlocality is the strongest type. Bell’s nonlocality for quantum states is usually detected by violation of some Bell’s inequalities, such as Clause-Horne-Shimony-Holt inequality for two qubits. Steering is a manifestation of nonlocality intermediate between entanglement and Bell’s nonlocality. This peculiar feature has led to a curious quantum phenomenon, the one-way Einstein-Podolsky-Rosen steering. The one-way steering was an important open question presented in 2007, and positively answered in 2014 by Bowles et al., who presented a simple class of one-way steerable states in a two-qubit system with at least thirteen projective measurements. The inspiring result for the first time theoretically confirms quantum nonlocality can be fundamentally asymmetric. Here, we propose another curious quantum phenomenon: Bell nonlocal states can be constructed from some steerable states. This novel finding not only offers a distinctive way to study Bell’s nonlocality without Bell’s inequality but with steering inequality, but also may avoid locality loophole in Bell’s tests and make Bell’s nonlocality easier for demonstration. Furthermore, a nine-setting steering inequality has also been presented for developing more efficient one-way steering and detecting some Bell nonlocal states.
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Authors: Tan Yu Jun , Tan Xipeng , Yeong Wai Yee , Tor Shu Beng 
Published: December 2016

AbstractA hybrid 3D bioprinting approach using porous microscaffolds and extrusion-based printing method is presented. Bioink constitutes of cell-laden poly(D,L-lactic-co-glycolic acid) (PLGA) porous microspheres with thin encapsulation of agarose-collagen composite hydrogel (AC hydrogel). Highly porous microspheres enable cells to adhere and proliferate before printing. Meanwhile, AC hydrogel allows a smooth delivery of cell-laden microspheres (CLMs), with immediate gelation of construct upon printing on cold build platform. Collagen fibrils were formed in the AC hydrogel during culture at body temperature, improving the cell affinity and spreading compared to pure agarose hydrogel. Cells were proven to proliferate in the bioink and the bioprinted construct. High cell viability up to 14 days was observed. The compressive strength of the bioink is more than 100 times superior to those of pure AC hydrogel. A potential alternative in tissue engineering of tissue replacements and biological models is made possible by combining the advantages of the conventional solid scaffolds with the new 3D bioprinting technology.
Water photolysis effect on the long-term stability of a fiber optic hydrogen sensor with Pt/WO3
Authors: Zhong Xuexiang , Yang Minghong , Huang Chujia , Wang Gaopeng , Dai Jixiang , Bai Wei 
Published: December 2016

AbstractOne of the technological challenges for hydrogen sensors is long-term stability and reliability. In this article, the UV-light irradiation was introduced into the hydrogen sensing process based on water photolysis effect of Pt/WO3. Ascribing to that, fiber optic hydrogen sensor with Pt/WO3 nanosheets as the sensing element was demonstrated with significantly improved performance of stability. Under UV irradiation, the hydrogen sensor exhibits higher sensitivity and resolution together with a smaller error range than that without UV irradiation. The enhanced performance could be attributed to the effective decomposition of water produced in the hydrogen sensing process due to the water photolysis effect of Pt/WO3. The influence of the water on stability was evaluated using experimental results, and the UV irradiation to remove water was analysed by theoretical and FT-IR spectra. This work provides new strategy of UV-light irradiation to promote the long-term stability of hydrogen sensor using Pt/WO3 as the sensing element.
The 5′UTR variant of ERCC5 fails to influence outcomes in ovarian and lung cancer patients undergoing treatment with platinum-based drugs
Authors: Rulli Eliana , Guffanti Federica , Caiola Elisa , Ganzinelli Monica , Damia Giovanna , Garassino Marina C. , Piva Sheila , Ceppi Lorenzo , Broggini Massimo , Marabese Mirko 
Published: December 2016

AbstractThe common polymorphic variant in the 5′ untranslated region of the excision repair cross-complementation group 5 (ERCC5) gene was described to generate an upstream open reading frame that regulates both the basal ERCC5 expression and its ability to be synthesized following DNA damage. This variant was reported to affect response to platinum therapy in a cohort of patients with pediatric ependymoma. The role of this variant was investigated in two cohorts of cancer patients, specifically in non-small-cell lung cancer (NSCLC) patients (N = 137) and in epithelial ovarian carcinoma (EOC) patients (N = 240), treated in first-line with platinum-based compounds. Differently from what reported for pediatric ependymoma, the analysis of the polymorphism in NSCLC patients cohort was not able to detect any difference among patients harboring different genotypes both in progression free survival (HR = 0.93; 95%CI 0.64–1.33; p-value = 0.678) and overall survival (HR = 0.90; 95%CI 0.62–1.33; p-value = 0.625). These data were corroborated in a EOC patients cohort, where similar progression free survival (HR = 0.91; 95% CI 0.67–1.24; p-value = 0.561) and overall survival (HR = 0.98; 95% CI 0.71–1.35; p-value = 0.912) were found for the different genotypes. These data, obtained in appropriately sized populations, indicate that the effect of this ERCC5 polymorphism is likely to be relevant only in specific tumors.
PD-L1+MDSCs are increased in HCC patients and induced by soluble factor in the tumor microenvironment
Authors: Iwata Tomoaki , Kondo Yasuteru , Kimura Osamu , Morosawa Tatsuki , Fujisaka Yasuyuki , Umetsu Teruyuki , Kogure Takayuki , Inoue Jun , Nakagome Yu , Shimosegawa Tooru 
Published: December 2016

AbstractMyeloid-derived suppressor cells (MDSCs) could have important roles in immune regulation, and MDSCs can be induced in patients with various malignant tumors. The immune-suppressive functions of MDSCs in hepatocellular carcinoma (HCC) patients have not been clarified. Therefore, we tried to analyze the biological significance of MDSCs in HCC patients. We quantified PD-L1+MDSCs of HCC patients in various conditions by using multi-color flow cytometry analysis. PBMCs from HCC patients contained significantly higher percentages of PD-L1+MDSCs in comparison to those from healthy subjects (p < 0.001). The percentages of PD-L1+MDSCs were reduced by curative treatment for HCC (p < 0.05), and the percentages of PD-L1+MDSCs before treatment were inversely correlated with disease-free survival time. After we cocultivated PBMCs and several liver cancer cell lines in a transwell coculture system, the percentages of PD-L1+MDSCs were significantly increased compared with control (p < 0.05). The expression of M-CSF and VEGFA was higher in the cell lines that strongly induced PD-L1+MDSCs. Peripheral blood from HCC patients had significantly higher percentages of PD-L1+MDSCs in comparison to those of healthy subjects, and the percentages of PD-L1+MDSCs were reduced by HCC treatment, suggesting that we might use PD-L1+MDSCs as a new biomarker of HCC.
Pure-Tone–Spondee Threshold Relationships in Functional Hearing Loss: A Test of Loudness Contribution
Authors: Schlauch Robert S. , Han Heekyung J. , Yu Tzu-Ling J. , Carney Edward 
Published: December 2016

Purpose
The purpose of this article is to examine explanations for pure-tone average–spondee threshold differences in functional hearing loss.

Method
Loudness magnitude estimation functions were obtained from 24 participants for pure tones (0.5 and 1.0 kHz), vowels, spondees, and speech-shaped noise as a function of level (20–90 dB SPL). Participants listened monaurally through earphones. Loudness predictions were obtained for the same stimuli by using a computational, dynamic loudness model.

Results
When evaluated at the same SPL, speech-shaped noise was judged louder than vowels/spondees, which were judged louder than tones. Equal-loudness levels were inferred from fitted loudness functions for the group. For the clinical application, the 2.1-dB difference between spondees and tones at equal loudness became a 12.1-dB difference when the stimuli were converted from SPL to HL.

Conclusions
Nearly all of the pure-tone average–spondee threshold differences in functional hearing loss are attributable to references for calibration for 0 dB HL for tones and speech, which are based on detection and recognition, respectively. The recognition threshold for spondees is roughly 9 dB higher than the speech detection threshold; persons feigning a loss, who base loss magnitude on loudness, do not consider this difference. Furthermore, the dynamic loudness model was more accurate than the static model.
Comprehensive Social Communication Support for Improving Transitions for Adolescents with Autism Spectrum Disorder (ASD)
Authors: Waller Jana , Sanford Marie , Caswell Tina , Bainbridge Christine 
Published: December 2016

Purpose
In this article we describe a comprehensive initiative for preparing adolescents with autism spectrum disorder (ASD) for successful transitions into adulthood. We present a framework for teaching and shaping social communication behaviors for bridging potential gaps in the progression from high school into postsecondary education, the workforce, and independent living.

Background
In our program, Information and Communication Readiness for All Transition Experiences (ICREATE), we integrate six fundamental components to build social communication behaviors for effective transitions into new experiences.

Outcomes
Participants in ICREATE demonstrate improvements in social communication behaviors, including self-awareness, social reciprocity, and self-advocacy behaviors. Throughout the program, participants apply and generalize their learned social communication behaviors to a variety of contexts.

Implications
The ICREATE program has significant implications for adolescents with ASD, their families, and their communities. The information we provide serves as an outline that can be modified and adapted for a variety of settings and populations.
Magnetic activity in the HARPS M dwarf sample
Authors: Astudillo-Defru N. , Delfosse X. , Bonfils X. , Forveille T. , Lovis C. , Rameau J. 
Published: December 2016

Context. Atmospheric magnetic fields in stars with convective envelopes heat stellar chromospheres, and thus increase the observed flux in the Ca ii H and K doublet. Starting with the historical Mount Wilson monitoring program, these two spectral lines have been widely used to trace stellar magnetic activity, and as a proxy for rotation period (Prot) and consequently for stellar age. Monitoring stellar activity has also become essential in filtering out false-positives due to magnetic activity in extra-solar planet surveys. The Ca ii emission is traditionally quantified through the R'HK-index, which compares the chromospheric flux in the doublet to the overall bolometric flux of the star. Much work has been done to characterize this index for FGK-dwarfs, but M dwarfs – the most numerous stars of the Galaxy – were left out of these analyses and no calibration of their Ca ii H and K emission to an R'HK exists to date.
Aims. We set out to characterize the magnetic activity of the low- and very-low-mass stars by providing a calibration of the R'HK-index that extends to the realm of M dwarfs, and by evaluating the relationship between R'HK and the rotation period.
Methods. We calibrated the bolometric and photospheric factors for M dwarfs to properly transform the S-index (which compares the flux in the Ca ii H and K lines to a close spectral continuum) into the R'HK. We monitored magnetic activity through the Ca ii H and K emission lines in the HARPS M dwarf sample.
Results. The R'HK index, like the fractional X-ray luminosity LX/Lbol, shows a saturated correlation with rotation, with saturation setting in around a ten days rotation period. Above that period, slower rotators show weaker Ca ii activity, as expected. Under that period, the R'HK index saturates to approximately 10-4. Stellar mass modulates the Ca ii activity, with R'HK showing a constant basal activity above 0.6 M⊙ and then decreasing with mass between 0.6 M⊙ and the fully-convective limit of 0.35 M⊙. Short-term variability of the activity correlates with its mean level and stars with higher R'HK indexes show larger R'HK variability, as previously observed for earlier spectral types.
Complex language, complex thought?
Authors: Bogaerds-Hazenberg Suzanne T.M. , Hendriks Petra 
Published: December 2016

Abstract
It has been argued (e.g., by De Villiers and colleagues) that the acquisition of sentence embedding is necessary for the development of first-order Theory of Mind (ToM): the ability to attribute beliefs to others. This raises the question whether the acquisition of double embedded sentences is related to, and perhaps even necessary for, the development of second-order ToM: the ability to attribute beliefs about beliefs to others. This study tested 55 children (aged 7-10) on their ToM understanding in a false-belief task and on their elicited production of sentence embeddings. We found that second-order ToM passers produced mainly double embeddings, whereas first-order ToM passers produced mainly single embeddings. Furthermore, a better performance on second-order ToM predicted a higher rate of double embeddings and a lower rate of single embeddings in the production task. We conclude that children’s ability to produce double embeddings is related to their development of second-order ToM.
Investigating the parent-child interactive behavior of autistic children by using composite light-emitting or reflective toys
Authors: Lee Ya-Hsueh , Ma Min-Yuan 
Published: December 2016

Abstract
A toy is a valuable medium for promoting parent-child interaction. This study selected six light-emitting or reflective materials to produce composite toy balls, and conducted tests on 15 families with preschool-aged high-functioning autistic children. Quantification method I analysis was employed in the study, and the experimental results indicated that (a) the metal ball (reflective and dynamic light) was the representative sample that elicited many smiles or laughs and much finger pointing as well as high levels of pleasure and activeness.; (b) the mitball (static light) was the representative sample that provoked much eye contact, produced satisfactory reactions to activities,; (c) the quality of fluorescence enhanced the levels of pleasure and the reactions to the activity. We propose that dynamic and reflective materials can be adopted to divert the attention of children, and fluorescent toys can then employed to sustain parent-child interaction.
Emotion-related musical variables affect person perception
Authors: Franco Fabia , Angelova Stanislava 
Published: December 2016

AbstractThis study investigated person perception in respect of variables associated with affect in music (tempo and mode) following motor synchronization to music. Participants (n = 128, 50% female) were tested in a task involving stepping with a researcher to the beat of slow or fast music in major or minor mode, following which measures concerning the synchronised partner were collected (incidental memory, likeability of, similarity to and prosocial attitude towards). Significant effects were found only for the ‘likeability’ measure, modulated by gender, suggesting that variables associated with affect perception in music, such as mode and tempo appear relevant for person perception in a synchronization task in ways that differ for men and women. The results of this ‘proof of concept’ study indicate the need for further research on the effect of motor synchronization based on ecologically valid musical interaction tasks.
Bifurcation of periodic solutions in a nonlinear difference-differential equations of neutral type
Authors: Brayton Robert K. 
Published: December 2016

The existence of a self-sustained periodic solution in the autonomous equation
\[

u
′

(
τ
)

−
α

u
′

(

τ
−
h

)

+
β
u

(
τ
)

+
α
γ
u

(

τ
−
h

)

=
ϵ
f

(

u

(
τ
)

)

u’\left ( \tau \right ) - \alpha u’\left ( {\tau - h} \right ) + \beta u\left ( \tau \right ) + \alpha \gamma u\left ( {\tau - h} \right ) = \epsilon f\left ( {u\left ( \tau \right )} \right )

\]
is proved under appropriate assumptions on

α
,
β
,
γ
,
f

\alpha ,\beta ,\gamma ,f

and

h
h

. The method of proof consists in converting this equation into an equivalent nonlinear integral equation and demonstrating the convergence of an appropriate iteration scheme.
Addition theorems for spherical waves
Authors: Friedman Bernard , Russek Joy 
Published: December 2016

Expansions or “addition theorems” for the spherical wave functions

j
n

(

k
R

)

P
n
m

(

cos
⁡
θ

)

exp
⁡

(

i
m
ϕ

)

{j_n}\left ( {kR} \right )P_n^m\left ( {\cos \theta } \right )\exp \left ( {im\phi } \right )

,

h
n

(
1
)

(

k
R

)

P
n
m

(

cos
⁡
θ

)

exp
⁡

(

i
m
ϕ

)

h_n^{\left ( 1 \right )}\left ( {kR} \right )P_n^m\left ( {\cos \theta } \right )\exp \left ( {im\phi } \right )

, and

h
n

(
2
)

(

k
R

)

P
n
m

(

cos
⁡
θ

)

exp
⁡

(

i
m
ϕ

)

h_n^{\left ( 2 \right )}\left ( {kR} \right )P_n^m\left ( {\cos \theta } \right )\exp \left ( {im\phi } \right )

, with reference to the origin

O
O

, have been obtained in terms of spherical wave functions with reference to the origin

O
′

O’

, where

O
′

O’

, has the coordinates

(

r
0

,

θ
0

,

ϕ
0

)

\left ( {{r_0},{\theta _0},{\phi _0}} \right )

with respect to

O
O

.
Image-based correction of continuous and discontinuous non-planar axial distortion in serial section microscopy
Authors: Hanslovsky Philipp , Bogovic John A , Saalfeld Stephan 
Published: December 2016

Abstract

Motivation
Serial section microscopy is an established method for detailed anatomy reconstruction of biological specimen. During the last decade, high resolution electron microscopy (EM) of serial sections has become the de-facto standard for reconstruction of neural connectivity at ever increasing scales (EM connectomics). In serial section microscopy, the axial dimension of the volume is sampled by physically removing thin sections from the embedded specimen and subsequently imaging either the block-face or the section series. This process has limited precision leading to inhomogeneous non-planar sampling of the axial dimension of the volume which, in turn, results in distorted image volumes. This includes that section series may be collected and imaged in unknown order.

Results
We developed methods to identify and correct these distortions through image-based signal analysis without any additional physical apparatus or measurements. We demonstrate the efficacy of our methods in proof of principle experiments and application to real world problems.

Availability and Implementation
We made our work available as libraries for the ImageJ distribution Fiji and for deployment in a high performance parallel computing environment. Our sources are open and available at http://github.com/saalfeldlab/section-sort, http://github.com/saalfeldlab/z-spacing and http://github.com/saalfeldlab/z-spacing-spark.

Supplementary information
Supplementary data are available at Bioinformatics online