bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reosseointegration Following Regenerative Therapy of Tissue‐Engineered Bone in a Canine Model of Experimental Peri‐Implantitis
Authors: Xu Lianyi , Sun Xiaojuan , Bai Jue , Jiang Li , Wang Shaoyi , Zhao Jun , Xia Lunguo , Zhang Xiuli , Wen Jin , Li Guanglong , Jiang Xinquan 
Published: February 2016

AbstractBackgroundDue to the existence of inflammation and limited osteogenesis on the precontaminated implant surface, reosseointegration is difficult to realize by current therapies. Tissue‐engineering strategy has been proved quite effective in intractable bone defect situation.PurposeThis study was designed to see whether the adoption of tissue‐engineered bone complex of adipose‐derived stem cells (ASCs) and bone morphogenetic protein‐2 (BMP‐2) gene delivery would work efficiently in the correction of experimental peri‐implantitis.MethodsAll premolars in both side of mandibular were removed from six beagle canines three months before implant placement. Typical peri‐implantitis were then induced by three month ligature placement. After the implementation of identical anti‐bacterial and mechanical debridement therapy, the shaped peri‐implant defect were stuffed with four groups of constructs, as A: beta tricalcium phosphate (β‐TCP); B: β‐TCP with ASCs; C: β‐TCP with enhanced green fluorescent protein gene transduced ASCs (AdGFP‐ASCs); and D: β‐TCP with bone morphogenetic protein‐2 gene‐modified ASCs (AdBMP‐2‐ASCs). Systematic radiographic, micro‐CT, and histomorphometrical assessments were performed.ResultsAfter six months of healing, more bone formation and reosseointegration was found around the implant of groups B and C than group A. And group D further promoted the new bone height and reosseointegration percentage. Moreover, sequential fluorescence labeling tells that group D exhibited the quickest and strongest bone formation on the cleaned implant surface during the entire observation period as compared to the other three groups.ConclusionsThese data demonstrated that tissue engineered bone of ASCs, BMP‐2 gene delivery, and β‐TCP could exert powerful therapeutic effect on peri‐implantitis as expected, which may suggest a feasible way to maintain the stability and masticatory function of dental implant.
Osseoinductive elements around immediate implants for better osteointegration: a pilot study in foxhound dogs
Authors: Salomó‐Coll O. , de Maté‐Sánchez J. E. Val , Ramírez‐Fernandez M. P. , Hernández‐Alfaro F. , Gargallo‐Albiol J. , Calvo‐Guirado J. L. 
Published: February 2016

AbstractObjectiveThe aim of this study was to evaluate the effects on osseointegration of topical applications of melatonin vs. vitamin D over surfaces of immediate implants.Materials and methodsMandibular premolar distal roots (P2, P3, P4) were extracted bilaterally from six American Foxhound dogs. Three conical immediate implants were randomly placed bilaterally in each mandible. Three randomized groups were created: melatonin 5% test group (MI), vitamin D 10% test group (DI), and Control group implants (CI). Block sections were obtained after 12 weeks and processed for mineralized ground sectioning. Bone‐to‐implant contact (total BIC), new bone formation (NBF), inter‐thread bone (ITB), and histological linear measurements (HLM) were assessed.ResultsAt 12 weeks, all implants were clinically stable and histologically osseointegrated. Total BIC values were 49.20 ± 3.26 for the MI group, 49.86 ± 1.89 for DI group and 45.78 ± 4.21 for the CI group (P < 0.018) with statistically significant difference between the three groups. BIC percentage were 42.44 ± 2.18 for MI, 44.56 ± 1.08 for DI, and 41.95 ± 3.34 for CI groups respectively (P > 0.05). Inter‐thread bone formation values were MI 17.56 ± 2.01, for DI 19.87 ± 0.92, and CI 14.56 ± 1.24 (P > 0.05). Statistically significant differences in peri‐implant new bone formation were found between the three groups: MI 28.76 ± 1.98, DI 32.56 ± 1.11 and CI 25.43 ± 4.67, respectively (P < 0.045). Linear measurements showed that the MI group showed significantly less lingual crestal bone loss (CBL) (MI 0.59 ± 0.71), compared to DI (0.91 ± 1.21) and CI (0.93 ± 1.21) (P < 0.042), and less lingual peri‐implant mucosa (PIM) (MI 3.11 ± 1.34),(DI 3.25 ± 0.18 compared with CI 3.54 ± 1.81 (P = 0.429). Linear measurements of buccal CBL showed significantly less buccal bone loss in test DI (0.36 ± 0.12) than CI (1.34 ± 1.23) and MI (1.11 ± 1.38) (P = 0.078).ConclusionsWithin the limitations of this animal study, topical applications of 5% Melatonin or 10% vitamin D improved bone formation around implants placed immediately after extraction and helped to reduce CBL after 12 weeks osseointegration.
Abutments with reduced diameter for both cement and screw retentions: analysis of failure modes and misfit of abutment–crown–connections after cyclic loading
Authors: Moris Izabela Cristina Maurício , Faria Adriana Cláudia Lapria , Ribeiro Ricardo Faria , Rodrigues Renata Cristina Silveira 
Published: February 2016

AbstractObjectiveThe aim of this study was to analyze failure modes and misfit of abutments with reduced diameter for both cement and screw retentions after cyclic loading.Material and MethodsForty morse‐taper abutment/implant sets of titanium were divided into four groups (N = 10): G4.8S‐4.8 abutment with screw‐retained crown; G4.8C‐4.8 abutment with cemented crown; G3.8S‐3.8 abutment with screw‐retained crown; and G3.8C‐3.8 abutment with cemented crown. Copings were waxed on castable cylinders and cast by oxygen gas flame and injected by centrifugation. After, esthetic veneering ceramic was pressed on these copings for obtaining metalloceramic crowns of upper canine. Cemented crowns were cemented on abutments with provisional cement (Temp Bond NE), and screw‐retained crowns were tightened to their abutments with torque recommended by manufacturer (10 N cm). The misfit was measured using a stereomicroscope in a 10× magnification before and after cyclic loading (300,000 cycles). Tests were visually monitored, and failures (decementation, screw loosening and fractures) were registered. Misfit was analyzed by mixed linear model while failure modes by chi‐square test (α = 0.05).ResultsCyclic loading affected misfit of 3.8C (P ≤ 0.0001), 3.8S (P = 0.0055) and 4.8C (P = 0.0318), but not of 4.8S (P = 0.1243). No differences were noted between 3.8S with 4.8S before (P = 0.1550) and after (P = 0.9861) cyclic loading, but 3.8C was different from 4.8C only after (P = 0.0015) loading. Comparing different types of retentions at the same diameter abutment, significant difference was noted before and after cyclic loading for 3.8 and 4.8 abutments. Analyzing failure modes, retrievable failures were present at 3.8S and 3.8C groups, while irretrievable were only present at 3.8S.ConclusionsThe cyclic loading decreased misfit of cemented and screw‐retained crowns on reduced diameter abutments, and misfit of cemented crowns is greater than screw‐retained ones. Abutments of reduced diameter failed more than conventional.
Long‐term quality‐of‐life outcomes following treatment for adult obstructive sleep apnoea: comparison of upper airway surgery, continuous positive airway pressure and mandibular advancement splints
Authors: Woods C.M. , Gunawardena I. , Chia M. , Vowles N.J. , Ullah S. , Robinson S. , Carney A.S. 
Published: February 2016

ObjectiveLong‐term quality‐of‐life (QOL) outcomes, complications and clinical effectiveness in patients undergoing treatment with upper airway surgery (UAS), continuous positive airway pressure (CPAP) and mandibular advancement splints (MAS) for adult obstructive sleep apnoea (OSA).DesignRetrospective cohort study.SettingMultidisciplinary OSA clinic in University teaching hospital.ParticipantsConsecutive, simultaneously treated patients with OSA undergoing UAS (n = 83), CPAP (n = 83) and MAS (n = 79).Main outcome measuresGlasgow Benefit Inventory (GBI), Snoring Severity Scale (SSS), Epworth Sleepiness Score (ESS) and side‐effects in all three groups were recorded at a mean of 34.5 months following start of treatment and compared via anova with Bonferroni's adjustment for pairwise comparisons.ResultsUpper airway surgery demonstrated a statistically significant QOL benefit over MAS. All three groups showed a significant improvement in SSS with CPAP significantly better than MAS, but equivalent to UAS. Uncomplicated UAS provided a greater QOL outcome than compliant MAS, non‐compliant CPAP (P < 0.05) and comparable to compliant CPAP. Patients undergoing UAS with recorded complications still reported equivalent QOL outcomes to compliant CPAP and MAS, suggesting these surgical complications are relatively minor compared to the QOL benefit of OSA treatment.ConclusionUpper airway surgery showed a significant improvement in QOL outcomes compared to non‐compliant CPAP or MAS and equivalent benefit to compliant CPAP. This study strongly supports the role for contemporary UAS in OSA when CPAP is not or no longer an option.
Clinicopathological characteristics and prognosis of schistosomal colorectal cancer
Authors: Wang M. , Wu Q. B. , He W. B. , Wang Z. Q. 
Published: February 2016

AbstractAimThe aim of the present study was to investigate the clinicopathological characteristics and prognostic factors of schistosomal colorectal cancer.MethodA total of 74 consecutive schistosomal colorectal cancer patients who underwent curative surgery from July 2009 to July 2012 were included in this study. The clinical and pathological characteristics of all 74 patients were analysed and univariate and multivariate analyses were performed. This study demonstrated positive correlations between the site of deposition of schistosomal eggs and certain essential variables.ResultsDepositional site of schistosome eggs, carcinoembryonic antigen (CEA) level and the pathological N and T stages were statistically significantly correlated with overall survival (OS). The pathological T stage and the CEA level were independent prognostic factors for OS. The site of deposition of schistosome eggs was positively correlated with the T and N stages, tumour size, the CEA level and the resection margins.ConclusionsSchistosome eggs might be associated with tumorigenesis. The site of deposition of schistosome eggs was statistically significantly correlated with OS but it was not an independent prognostic factor for OS. It was, however, correlated with the depth of the tumour. The presence of schistosoma eggs at the margin did not affect the patient's prognosis or anastomotic healing. The existing standard surgical approach was equally applicable to schistosomal colorectal cancer. It was not necessary to expand the scope of surgical resection.
SPOCK1 is upregulated in recurrent glioblastoma and contributes to metastasis and Temozolomide resistance
Authors: Yu Fengbo , Li Guihong , Gao Junxia , Sun Yuxue , Liu Pengfei , Gao Haijun , Li Peiwen , Lei Ting , Chen Yong , Cheng Ye , Zhai Xiao , Sayari Arash. J. , Huang Haiyan , Mu Qingchun 
Published: February 2016

AbstractObjectivesGlioblastoma multiforme (GBM) is the most aggressive brain tumour type in humans. Its poor prognosis is largely attributed to its invasiveness and high rate of recurrence. Recurring GBM is commonly resistant to chemotherapeutic drugs, making it specially difficult to treat. Recent studies have revealed that matricellular glycoprotein SPOCK1 to be upregulated in several cancer types and to be specifically expressed in invasive GBM, but not in other types of non‐invasive brain tumour, which prompted us to study the mechanism of action of SPOCK1 in invasion, recurrence and drug resistance of GBM cells.Materials and methodsSPOCK1 expression in GBM tissues was evaluated using qPCR, Western blotting and immunohistochemical staining. Cell migration was tested by the wound healing method and cell invasion was assessed using transwell plates with Matrigel coating. Western blotting was performed for E‐cadherin, vimentin, N‐cadherin, p‐Akt and Akt. Cell viability was examined using the MTT assay.ResultsWe found that the expression of SPOCK1 was significantly upregulated in recurrent GBM. We also demonstrated that SPOCK1 positively regulated migration, invasion and EMT process of GBM cells. Furthermore, SPOCK1 mediated TMZ resistance in GBM, as knockdown of SPOCK1 expression in TMZ‐resistant GBM cells substantially sensitized these cells to TMZ.ConclusionSPOCK1 results were positive and it mediated TMZ resistance in GBM. In addition, SPOCK1 regulated invasion and TMZ resistance in GBM cells via the Akt signalling pathway.
The epidemiology of amyotrophic lateral sclerosis in the Mount Etna region: a possible pathogenic role of volcanogenic metals
Authors: Nicoletti A. , Vasta R. , Venti V. , Mostile G. , Lo Fermo S. , Patti F. , Scillieri R. , De Cicco D. , Volanti P. , Marziolo R. , Maimone D. , Fiore M. , Ferrante M. , Zappia M. 
Published: February 2016

Background and purposeTrace elements (TEs) may play a role in the pathogenesis of amyotrophic lateral sclerosis (ALS) and volcanic degassing is the major natural source of TEs. Mount Etna, in the province of Catania, is the largest active volcano in Europe. Our aim was to assess the incidence of ALS in the province of Catania during 2005–2010 and its spatial distribution with respect to volcanic gas deposition.MethodsCases from all neurological centres of the province of Catania and of the boundary provinces were retrospectively collected. Patients who had onset during 2005–2010 and fulfilled the El Escorial revised diagnostic criteria were included. The incidence of ALS was estimated for the entire province and separately for the population living on the eastern and western flank of Mount Etna, respectively, the most and least exposed areas to volcanogenic TEs, considered as a possible risk factor for ALS.ResultsOne hundred and twenty‐six (57 men) ALS patients were enrolled. The mean annual crude incidence rate was 2.0/100 000 person‐years (95% confidence interval 1.7–2.4). A higher incidence rate was found in the population living on the eastern flank compared to the western flank (2.4/100 000 and 0.9/100 000 respectively) with a relative risk of 2.75 (95% confidence interval 1.64–4.89; P < 0.001).ConclusionsThe incidence of ALS in the province of Catania is close to those reported worldwide. The incidence was higher amongst the population living on the eastern flank of Mount Etna, which could be interpreted as a possible role of volcanogenic TEs. Further research on TEs and genetic factors is necessary to support this assumption.
Risky Courtship: Background Contrast, Ornamentation, and Display Behavior of Wolf Spiders Affect Visual Detection by Toad Predators
Authors: Clark David L. , Kizer Zeeff Corinna , Karson Adam , Roberts J. Andrew , Uetz George W. 
Published: February 2016

AbstractMales that search widely for females and perform conspicuous courtship displays run a high risk of being detected by their predators. Therefore, gains in reproductive success might be offset by increased mortality due to predation. Male brush‐legged wolf spiders (Schizocosa ocreata) with larger decorative traits (foreleg tufts) are preferred by females as mates, but are more readily detected by predators. However, predation risk may also be influenced by the interaction between components of signals and the environment in which signaling occurs. Courting male spiders were readily accepted as prey by a sympatric predator, the American toad (Anaxyrus americanus). We used video playback to tease apart the interactive effect between visual signals and the signaling environment on the ability of toads to detect courting spiders as a function of distance, background contrast, the presence or absence of male foreleg tufts, and behavioral activity. The response of toads to video sequences of male spiders was similar to their response to live male spiders. Toad response varied over distance toward spiders displayed against high contrast (sunny) vs. low contrast (shaded) backgrounds. Beyond 30 cm, more toads detected courting male spiders against light, ‘sunny’ backgrounds and detected them faster when compared to the same spider stimulus against darker, ‘shady’ backgrounds. In choice tests, toads oriented more often toward courting males with leg tufts than those without. Toad responses also varied with male spider behavior in that only videos of moving males were attacked. Latency to orient and detection by toads was significantly greater for walking males than courting males, and this effect was most evident at distances between 30 cm and 50 cm. Results supported that courting wolf spiders are at significant risk of predation by visually acute predators. Distance, background contrast, and the presence of foreleg decorations influence detection probability. Thus, the same complex visual signals that make males conspicuous and are preferred by females can make males more vulnerable as prey to toads.
The aetiopathogenesis of equine periodontal disease – a fresh perspective
Authors: Kennedy R. S. , Dixon P. M. 
Published: February 2016

SummaryPeriodontal disease is a painful and highly prevalent disorder of horses that causes a significant welfare problem. Despite its importance, few scientific studies on its aetiopathogenesis have been performed. Equine periodontitis differs from the plaque‐induced periodontitis found in brachydont species where bacteria accumulating in dental plaque induce a destructive inflammatory response in the periodontium. In contrast, equine periodontitis is usually initiated by entrapment of feed between cheek teeth, which causes inflammation of periodontal tissue that likely allows bacterial infection of the periodontal tissues that is later exacerbated by the host's response. Equine oral microbiology is a neglected field of research and identification of the bacteria involved in this disorder by use of molecular bacteriology and examination of the interaction between these bacteria and the equine oral immune response should reveal important information about the pathogenesis of this disease.
Clinical screening of horses and early warning for West Nile virus
Authors: Leblond A. , Lecollinet S. 
Published: February 2016

SummaryClinical presentation of West Nile disease in horses is variable, but ataxia, weakness and muscle fasciculations are often observed, sometimes with abnormal behaviour, teeth grinding and bruxism. Practitioners should be aware that horses are more sensitive to infection than man and serve as sentinels for an early warning of West Nile virus circulation in a given area. This early warning allows the implementation of preventive and control measures such as vaccination of horses and mosquito control.
Lithostratigraphic analysis of a new stromatolite–thrombolite reef from across the rise of atmospheric oxygen in the Paleoproterozoic Turee Creek Group, Western Australia
Authors: Barlow E. , Van Kranendonk M. J. , Yamaguchi K. E. , Ikehara M. , Lepland A. 
Published: February 2016

AbstractThis study describes a previously undocumented dolomitic stromatolite–thrombolite reef complex deposited within the upper part (Kazput Formation) of the c. 2.4–2.3 Ga Turee Creek Group, Western Australia, across the rise of atmospheric oxygen. Confused by some as representing a faulted slice of the younger c. 1.8 Ga Duck Creek Dolomite, this study describes the setting and lithostratigraphy of the 350‐m‐thick complex and shows how it differs from its near neighbour. The Kazput reef complex is preserved along 15 km of continuous exposure on the east limb of a faulted, north‐west‐plunging syncline and consists of 5 recognisable facies associations (A–E), which form two part regressions and one transgression. The oldest facies association (A) is characterised by thinly bedded dololutite–dolarenite, with local domical stromatolites. Association B consists of interbedded columnar and stratiform stromatolites deposited under relatively shallow‐water conditions. Association C comprises tightly packed columnar and club‐shaped stromatolites deposited under continuously deepening conditions. Clotted (thrombolite‐like) microbialite, in units up to 40 m thick, dominates Association D, whereas Association E contains bedded dololutite and dolarenite, and some thinly bedded ironstone, shale and black chert units. Carbon and oxygen isotope stratigraphy reveals a narrow range in both δ13Ccarb values, from −0.22 to 0.97‰ (VPDB: average = 0.68‰), and δ18O values, from −14.8 to −10.3‰ (VPDB), within the range of elevated fluid temperatures, likely reflecting some isotopic exchange. The Kazput Formation stromatolite–thrombolite reef complex contains features of younger Paleoproterozoic carbonate reefs, yet is 300–500 Ma older than previously described Proterozoic examples worldwide. Significantly, the microbial fabrics are clearly distinct from Archean stromatolitic marine carbonate reefs by way of containing the first appearance of clotted microbialite and large columnar stromatolites with complex branching arrangements. Such structures denote a more complex morphological expression of growth than previously recorded in the geological record and may link to the rise of atmospheric oxygen.
Proteolytic and angiotensin‐converting enzyme‐inhibitory activities of selected probiotic bacteria
Authors: Ahtesh Fatah B. , Stojanovska Lily , Mathai Michael L. , Apostolopoulos Vasso , Mishra Vijay K. 
Published: February 2016

SummaryThis study was carried out to examine the proteolytic and angiotensin‐converting enzyme (ACE‐I) activities of probiotic lactic acid bacteria (LAB) as influenced by the type of media, fermentation time, strain type and media supplementation with a proteolytic enzyme (Flavourzyme®). Lactobacillus casei (Lc210), Bifidobacterium animalis ssp12 (Bb12), Lactobacillus delbrueckii subsp. bulgaricus (Lb11842) and Lactobacillus acidophilus (La2410) were grown in 12% of reconstituted skim milk (RSM) or 4% of whey protein concentrates (WPC‐35) with or without combination (0.14%) of Flavourzyme® for 12 h at 37 °C. All the strains were able to grow in both media depending on type of strains used and fermentation time. All the strains showed higher proteolytic activity and produced more antihypersensitive peptides when grown in RSM medium at 12 h, when compared to WPC. Combination with Flavourzyme® also increased LAB growth and proteolytic and ACE‐I activities. Of the four strains used, Bb12 and La2410 outperformed Lc210 and Lb11842. The highest ACE‐I activity and proteolytic activity were found in B. animalis ssp12 combined with Flavourzyme®. Flavourzyme® led to an increase in the production of bioactive peptides with ACE‐I activity during 12 h at 37 °C.