bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of a Meso-Alpine collision event on the tectono-metamorphic evolution of the Peloritani mountain belt (eastern Sicily, southern Italy)
Authors: CATALANO STEFANO , CIRRINCIONE ROSOLINO , MAZZOLENI PAOLO , PAVANO FRANCESCO , PEZZINO ANTONIO , ROMAGNOLI GINO , TORTORICI GIUSEPPE 
Published: June 2017

AbstractThe Peloritani Mountains, in the southern part of the Calabrian Terranes, southern Italy, have been classically interpreted as the product of the Paleogene brittle deformation of the European continental back-stop of the Neotethyan subduction complex. This reconstruction conflicts with the occurrence of an Alpine metamorphic overprint that affected portions of both the Variscan metamorphic units and part of the Mesozoic sedimentary covers of the mountain belt. New field data, integrated with petrographic, micro- and meso-structural analyses and stratigraphic investigation of the syn-tectonic terrigenous covers, well constrain a Paleogene collision event along the Africa–Nubia convergent margin that caused the exhumation of the Alpine metamorphic units of the Peloritani Mountains. The syn-collisional exhumation was associated with shearing along two major Africa-verging crustal thrusts arising from the positive tectonic inversion of the former European palaeomargin. Early tectonic motions occurred within the mountain belts and produced the exhumation of the external portions of the edifice. Later tectonic motions occurred along the sole-thrust of the entire edifice and caused the definitive exhumation of the entire mountain belt. The whole crustal thrusting lasted for a period ofc. 10 Ma, during the entire Oligocene. The definitive southwestward emplacement of the Peloritani Mountain Belt onto the Neotethyan accretionary wedge was followed by two Late Oligocene – Early Miocene NW–SE-oriented right lateral shear zones, replacing the previous crustal thrust. These two strike-slip belts are interpreted as the surface expression of the deep-seated suture zone between the colliding Africa and Europe continental crusts.
INTERNET CASES IN EU PRIVATE INTERNATIONAL LAW—DEVELOPING A COHERENT APPROACH
Authors: Lutzi Tobias 
Published: June 2017

AbstractInternet communication has long been known to pose a challenge to private international law and its reliance on geographical connecting factors. This article looks at the problem from the perspective of EU private international law and argues that the way in which it has been accommodated by Regulations Brussels I, Rome I, and II conflicts with some of its central paradigms. It advances an alternative approach that would generally submit claims against information society service providers established in the EU to the jurisdiction and substantive laws of their ‘country of origin’ but make certain exceptions for private persons and consumers. The article argues that implementing such an approach would require little legislative change, be more faithful to the particularities of internet communication, and give greater effect to the central paradigms of EU private international law.
A hundred years’ tale: rediscovery ofMollinedia stenophylla(Monimiaceae) in the Atlantic rainforest, Brazil
Authors: de Lírio Elton John , Freitas Joelcio , Negrão Raquel , Martinelli Gustavo , Peixoto Ariane Luna 
Published: June 2017

AbstractThe plantMollinedia stenophyllaPerkins (Monimiaceae) is endemic to southern Brazil and, until 2015, had not been seen for 122 years. We located a single population of the species on the margin of a watercourse in the mountainous region of Rio de Janeiro state. We describe the location of the species, comment on its morphology, ecology and conservation, assess its conservation status, propose conservation measures, and discuss the potential value of local action plans for this and other narrow endemic plant species.Mollinedia stenophyllahas whorled leaves, clonal reproduction and a low height, unusual traits in the genusMollinedia.Using the IUCN Red List criteria we assess the species as Critically Endangered. This example highlights the importance of investing time in plant surveys and taxonomy, especially in megadiverse countries such as Brazil.
‘Moving past the feeling’: emotion in Arcade Fire'sFuneral
Authors: Spitzer Michael 
Published: June 2017

AbstractThis article focuses on Arcade Fire's 2004 first album,Funeral. It is analytical in orientation, offering close (analytical) readings of Funeral's 10 tracks from the standpoint of reception of the album as particularly ‘emotional’. In order to explain Funeral's generation of extreme emotion, the article applies tools from current formal theory, particularly the theory of sentence phrase-structure. It connects sentence form with Spicer's notion of (ac)cumulative processes. With a nod to Osborn's article on ‘terminal climax’, it shows how Arcade Fire's sentential forms are unusually directed towards anthemic climaxes, associated with emotional breakthrough. The article also explores the tension in the music, grounded in the indie ethos in general, between ‘spectacular’ stasis and forward motion.
Initial assessment of a novel delivery system (NuDEL™®) for the covered Cheatham-Platinum stent
Authors: Morgan Gareth J. , Kenny Damien , Duke Christopher , Walsh Kevin P. , Qureshi Shakeel A. 
Published: June 2017

AbstractAimsWe sought to evaluate the first-in-man use of a new system for implantation of covered stents in patients with complex structural and CHD.Methods and resultsRetrospective data were collected of the first 13 NuDEL™ delivery systems used in patients. The NuDEL™ comprises a covered Cheatham-Platinum stent mounted on a balloon-in-balloon and pre-loaded in a long delivery sheath. Data were collected from three centres in the United Kingdom and Ireland. A total of 13 covered stents were delivered via 12 NuDEL™ delivery systems in 12 patients. Among them, six patients had coarctation of the aorta, five patients had right ventricular outflow tract stenosis, and one patient had severe stenosis of a Mustard systemic venous baffle. There were no complications, and all the stents were deployed in the desired position with satisfactory haemodynamic results.ConclusionsThe development of a bespoke system of a pre-mounted, pre-loaded covered stent may negate some of the technical challenges that complicate large-calibre stent deployment. Our preliminary results suggest that the NuDEL™ system is a safe and effective means of covered stent deployment in challenging anatomy.
Mass-Gathering Medical Care in Electronic Dance Music Festivals
Authors: FitzGibbon Kathleen M. , Nable Jose V. , Ayd Benjamin , Lawner Benjamin J. , Comer Angela C. , Lichenstein Richard , Levy Matthew J. , Seaman Kevin G. , Bussey Ian 
Published: June 2017

AbstractIntroductionElectronic dance music (EDM) festivals represent a unique subset of mass-gathering events with limited guidance through literature or legislation to guide mass-gathering medical care at these events.Hypothesis/ProblemElectronic dance music festivals pose unique challenges with increased patient encounters and heightened patient acuity under-estimated by current validated casualty predication models.MethodsThis was a retrospective review of three separate EDM festivals with analysis of patient encounters and patient transport rates. Data obtained were inserted into the predictive Arbon and Hartman models to determine estimated patient presentation rate and patient transport rates.ResultsThe Arbon model under-predicted the number of patient encounters and the number of patient transports for all three festivals, while the Hartman model under-predicted the number of patient encounters at one festival and over-predicted the number of encounters at the other two festivals. The Hartman model over-predicted patient transport rates for two of the three festivals.ConclusionElectronic dance music festivals often involve distinct challenges and current predictive models are inaccurate for planning these events. The formation of a cohesive incident action plan will assist in addressing these challenges and lead to the collection of more uniform data metrics.FitzGibbonKM, NableJV, AydB, LawnerBJ, ComerAC, LichensteinR, LevyMJ, SeamanKG, BusseyI. Mass-gathering medical care in electronic dance music festivals. Prehosp Disaster Med. 2017;32(5):563–567.
Titanium particle-induced osteogenic inhibition and bone destruction are mediated by the GSK-3β/β-catenin signal pathway
Authors: Gu Ye , Wang Zhirong , Shi Jiawei , Wang Liangliang , Hou Zhenyang , Guo Xiaobin , Tao Yunxia , Wu Xiexing , Zhou Wei , Liu Yu , Zhang Wen , Xu Yaozeng , Yang Huilin , Xue Feng , Geng Dechun 
Published: June 2017

AbstractWear debris-induced osteogenic inhibition and bone destruction are critical in the initiation of peri-prosthetic osteolysis. However, the molecular mechanism underlying this phenomenon is poorly understood. In this study, we analyzed the involvement of the GSK-3β/β-catenin signal pathway, which is important for bone formation in this pathological condition. We established a titanium (Ti) particle-stressed murine MC3T3-E1 cell culture system and calvariae osteolysis model to test the hypothesis that Ti particle-induced osteogenic inhibition and bone destruction are mediated by the GSK-3β/β-catenin signal pathway. Our findings showed that Ti particles reduced osteogenic differentiation induced by osteogenesis-related gene expression, alkaline phosphatase activity and matrix mineralization, as well as pSer9-GSK-3β expression and β-catenin signal activity. Downregulation of GSK-3β activity attenuated Ti particle-induced osteogenic inhibition, whereas the β-catenin inhibitor reversed this protective effect. Moreover, the GSK-3β/β-catenin signal pathway mediated the upregulation of RANKL and downregulation of OPG in Ti particle-stressed MC3T3-E1 cells. In addition, our in vivo results showed that Ti particles induced bone loss via regulating GSK-3β and β-catenin signals. Based on these results, we concluded that the GSK-3β/β-catenin signal pathway mediates the adverse effects of Ti particles on osteoblast differentiation and bone destruction, and can be used as a potential therapeutic target for the treatment of peri-prosthetic osteolysis.
Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
Authors: Kim Sae-Hae , Cho Byeol-Hee , Kiyono Hiroshi , Jang Yong-Suk 
Published: June 2017

AbstractThe regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intestine. Given that PP ILC3s are closely associated with mucosal immune regulation, it is important to characterize the regulatory mechanism of ILC3s. Here, we found that terminal ileal PPs of specific pathogen-free (SPF) mice have fewer NKp46+ ILC3s than jejunal PPs, while there was no difference in NKp46+ ILC3 numbers between terminal ileal and jejunal PPs in antibiotics (ABX)-treated mice. We also found that butyrate levels in the terminal ileal PPs of SPF mice were higher than those in the jejunal PPs of SPF mice and terminal ileal PPs of ABX-treated mice. The reduced number of NKp46+ ILC3s in terminal ileal PPs resulted in a decrease in Csf2 expression and, in turn, resulted in reduced regulatory T cells and enhanced antigen-specific T-cell proliferation. Thus, we suggest that NKp46+ ILC3s are negatively regulated by microbiota-derived butyrate in terminal ileal PPs and the reduced ILC3 frequency is closely associated with antigen-specific immune induction in terminal ileal PPs.
Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling
Authors: Wu Ting , Zhang Juan , Geng Mingxing , Tang Shao-Jun , Zhang Wenping , Shu Jianhong 
Published: June 2017

AbstractHAART is very effective in suppressing HIV-1 replication in patients. However, patients staying on long-term HAART still develop various HIV-associated neurological disorders, even when the viral load is low. The underlying pathogenic mechanisms are largely unknown. Emerging evidence implicated that persistent neuroinflammation plays an important role in NeuroAIDS. Although residual virus or viral proteins are commonly thought as the causal factors, we are interested in the alternative possibility that HAART critically contributes to the neuroinflammation in the central nervous system (CNS). To test this hypothesis, we have determined the effect of NRTIs on the expression of proinflammatory cytokines in the various CNS regions. Mice (C57Bl/6) were administered with AZT (Zidovudine 100 mg/kg/day), 3TC (Lamivudine 50 mg/kg/day) or D4T (Stavudine 10 mg/kg/day) for 5 days, and cortices, hippocampi and spinal cords were collected for immunoblotting. Our results showed that NRTI administration up-regulated cytokines, including IL-1β, TNF-α and IL-6 in various CNS regions. In addition, we found that NRTIs also up-regulated Wnt5a protein. Importantly, BOX5 attenuated NRTI-induced cytokine up-regulation. These results together suggest that NRTIs up-regulate proinflammatory cytokines via a Wnt5a signaling-dependent mechanism. Our findings may help understand the potential pathogenic mechanisms of HAART-associated NeuroAIDS and design effective adjuvants.
Development of lysosome-mimicking vesicles to study the effect of abnormal accumulation of sphingosine on membrane properties
Authors: Carreira Ana C. , de Almeida Rodrigo F. M. , Silva Liana C. 
Published: June 2017

AbstractSynthetic systems are widely used to unveil the molecular mechanisms of complex cellular events. Artificial membranes are key examples of models employed to address lipid-lipid and lipid-protein interactions. In this work, we developed a new synthetic system that more closely resembles the lysosome – the lysosome-mimicking vesicles (LMVs) – displaying stable acid-to-neutral pH gradient across the membrane. To evaluate the advantages of this synthetic system, we assessed the distinct effects of sphingosine (Sph) accumulation in membrane structure and biophysical properties of standard liposomes (no pH gradient) and in LMVs with lipid composition tuned to mimic physiological- or NPC1-like lysosomes. Ternary 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/Sphingomyelin (SM)/Cholesterol (Chol) mixtures with, respectively, low and high Chol/SM levels were prepared. The effect of Sph on membrane permeability and biophysical properties was evaluated by fluorescence spectroscopy, electrophoretic and dynamic light scattering. The results showed that overall Sph has the ability to cause a shift in vesicle surface charge, increase membrane order and promote a rapid increase in membrane permeability. These effects are enhanced in NPC1- LMVs. The results suggest that lysosomal accumulation of these lipids, as observed under pathological conditions, might significantly affect lysosomal membrane structure and integrity, and therefore contribute to the impairment of cell function.
The CA19-9 and Sialyl-TRA Antigens Define Separate Subpopulations of Pancreatic Cancer Cells
Authors: Barnett Daniel , Liu Ying , Partyka Katie , Huang Ying , Tang Huiyuan , Hostetter Galen , Brand Randall E. , Singhi Aatur D. , Drake Richard R. , Haab Brian B. 
Published: June 2017

AbstractMolecular markers to detect subtypes of cancer cells could facilitate more effective treatment. We recently identified a carbohydrate antigen, named sTRA, that is as accurate a serological biomarker of pancreatic cancer as the cancer antigen CA19-9. We hypothesized that the cancer cells producing sTRA are a different subpopulation than those producing CA19-9. The sTRA glycan was significantly elevated in tumor tissue relative to adjacent pancreatic tissue in 3 separate tissue microarrays covering 38 patients. The morphologies of the cancer cells varied in association with glycan expression. Cells with dual staining of both markers tended to be in well-to-moderately differentiated glands with nuclear polarization, but exclusive sTRA staining was present in small clusters of cells with poor differentiation and large vacuoles, or in small and ill-defined glands. Patients with higher dual-staining of CA19-9 and sTRA had statistically longer time-to-progression after surgery. Patients with short time-to-progression (<2 years) had either low levels of the dual-stained cells or high levels of single-stained cells, and such patterns differentiated short from long time-to-progression with 90% (27/30) sensitivity and 80% (12/15) specificity. The sTRA and CA19-9 glycans define separate subpopulations of cancer cells and could together have value for classifying subtypes of pancreatic adenocarcinoma.
Temperature dependence of protein-water interactions in a gated yeast aquaporin
Authors: Aponte-Santamaría Camilo , Fischer Gerhard , Båth Petra , Neutze Richard , de Groot Bert L. 
Published: June 2017

AbstractRegulation of aquaporins is a key process of living organisms to counteract sudden osmotic changes. Aqy1, which is a water transporting aquaporin of the yeast Pichia pastoris, is suggested to be gated by chemo-mechanical stimuli as a protective regulatory-response against rapid freezing. Here, we tested the influence of temperature by determining the X-ray structure of Aqy1 at room temperature (RT) at 1.3 Å resolution, and by exploring the structural dynamics of Aqy1 during freezing through molecular dynamics simulations. At ambient temperature and in a lipid bilayer, Aqy1 adopts a closed conformation that is globally better described by the RT than by the low-temperature (LT) crystal structure. Locally, for the blocking-residue Tyr31 and the water molecules inside the pore, both LT and RT data sets are consistent with the positions observed in the simulations at room-temperature. Moreover, as the temperature was lowered, Tyr31 adopted a conformation that more effectively blocked the channel, and its motion was accompanied by a temperature-driven rearrangement of the water molecules inside the channel. We therefore speculate that temperature drives Aqy1 from a loosely- to a tightly-blocked state. This analysis provides high-resolution structural evidence of the influence of temperature on membrane-transport channels.
Chemokine receptor CXCR4 regulates CaMKII/CREB pathway in spinal neurons that underlies cancer-induced bone pain
Authors: Hu Xue-Ming , Zhang Hui , Xu Heng , Zhang Hai-Long , Chen Li-Ping , Cui Wen-Qiang , Yang Wei , Shen Wen 
Published: June 2017

AbstractWe previously demonstrated that the chemokine receptor CXCR4 plays an important role in cancer-induced bone pain by activating spinal neurons and glial cells. However, the specific neuronal mechanism of CXCR4 signaling is not clear. We further report that CXCR4 contributes to the activation of the neuronal CaMKII/CREB pathway in cancer-induced bone pain. We used a tumor cell implantation (TCI) model and observed that CXCR4, p-CaMKII and p-CREB were persistently up-regulated in spinal neurons. CXCR4 also co-expressed with p-CaMKII and p-CREB, and mediated p-CaMKII and p-CREB expression after TCI. Intrathecal delivery of CXCR4 siRNA or CaMKII inhibitor AIP2 abrogated TCI-induced pain hypersensitivity and TCI-induced increase in p-CaMKII and p-CREB expression. Intrathecal injection of the principal ligand for CXCR4, SDF-1, promoted p-CaMKII and p-CREB expression in naive rats, which was prevented by post-administration of CXCR4 inhibitor Plerixafor or PLC inhibitor U73122. Plerixafor, U73122, or AIP2 also alleviated SDF-1-elicited pain behaviors. Intrathecal injection of CXCR4 siRNA significantly suppressed TCI-induced up-regulation of NMDAR1 mRNA and protein, which is a known gene target of CREB. Collectively, these results suggest that the CaMKII/CREB pathway in spinal neurons mediates CXCR4-facilitated pain hypersensitivity in cancer rats.
The stress response factor daf-16/FOXO is required for multiple compound families to prolong the function of neurons with Huntington’s disease
Authors: Farina Francesca , Lambert Emmanuel , Commeau Lucie , Lejeune François-Xavier , Roudier Nathalie , Fonte Cosima , Parker J. Alex , Boddaert Jacques , Verny Marc , Baulieu Etienne-Emile , Neri Christian 
Published: June 2017

AbstractHelping neurons to compensate for proteotoxic stress and maintain function over time (neuronal compensation) has therapeutic potential in aging and neurodegenerative disease. The stress response factor FOXO3 is neuroprotective in models of Huntington’s disease (HD), Parkinson’s disease and motor-neuron diseases. Neuroprotective compounds acting in a FOXO-dependent manner could thus constitute bona fide drugs for promoting neuronal compensation. However, whether FOXO-dependent neuroprotection is a common feature of several compound families remains unknown. Using drug screening in C. elegans nematodes with neuronal expression of human exon-1 huntingtin (128Q), we found that 3ß-Methoxy-Pregnenolone (MAP4343), 17ß-oestradiol (17ßE2) and 12 flavonoids including isoquercitrin promote neuronal function in 128Q nematodes. MAP4343, 17ßE2 and isoquercitrin also promote stress resistance in mutant Htt striatal cells derived from knock-in HD mice. Interestingly, daf-16/FOXO is required for MAP4343, 17ßE2 and isoquercitrin to sustain neuronal function in 128Q nematodes. This similarly applies to the GSK3 inhibitor lithium chloride (LiCl) and, as previously described, to resveratrol and the AMPK activator metformin. Daf-16/FOXO and the targets engaged by these compounds define a sub-network enriched for stress-response and neuronally-active pathways. Collectively, these data highlights the dependence on a daf-16/FOXO-interaction network as a common feature of several compound families for prolonging neuronal function in HD.
Laser-ultrasonic delivery of agents into articular cartilage
Authors: Nieminen Heikki J. , Barreto Gonçalo , Finnilä Mikko A. , García-Pérez Alejandro , Salmi Ari , Ranjan Sanjeev , Eklund Kari K. , Pritzker Kenneth P. H. , Saarakkala Simo , Hæggström Edward 
Published: June 2017

AbstractResearch is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induced plasma sound source) to deliver agents (methylene blue, MB, in PBS) into bovine AC. Treatment samples (n = 10) were immersed in MB solution simultaneously with LU exposure, while adjacent control 1 tissue (n = 10) was pre-treated with LU followed by immersion in MB and adjacent control 2 tissue (n = 10) was only immersed in MB. AC exposed (n = 22) or not exposed (n = 27) to LU were characterized for anomalies in structure, composition, viability or RNA expression. Optically detected MB content was significantly (p < 0.01) higher in treatment samples up to a depth of 500 µm from AC surface as compared to controls. No major unwanted short-term effects on AC structure, proteoglycan or collagen contents, chondrocyte viability or RNA expression levels were detected. In conclusion, LU can deliver agents into AC without major short-term concerns on safety. LU could reveal new strategies for the development of localized drug therapies in AC.